bluuebunny/crossref_metadata_2025_split huggingface dataset:
Loss-of-function ofFbxo10, encoding a post-translational regulator of BCL2 in lymphomas, has no discernible effect on BCL2 or B lymphocyte accumulation in mice
Authors: Masle-Farquhar Etienne , Russell Amanda , Li Yangguang , Zhu Fen , Rui Lixin , Brink Robert , Goodnow Christopher C 
Published: August 2020

AbstractRegulation of the anti-apoptotic BCL2 protein determines cell survival and is frequently abnormal in B cell lymphomas. An evolutionarily conserved post-translational mechanism for over-expression of BCL2 in human B cell lymphomas and the BCL2 paralogue CED-9 inCaenorhabditis elegansresults from loss-of-function mutations in human FBXO10 and itsC.elegansparalogue DRE-1, a BCL2/CED-9-binding subunit of the SKP-CULLIN-FBOX (SCF) ubiquitin ligase. Here, we tested the role of FBXO10 in BCL2 regulation by producing mice with two different CRISPR/Cas9-engineeredFbxo10mutations: an Asp54Lys (E54K) missense mutation in the FBOX domain and a Cys55SerfsTer55 frameshift (fs) truncating mutation. Mice homozygous for either mutant allele were born at the expected Mendelian frequency and appeared normal in body weight and appearance as adults. Spleen B cells from homozygous mutant mice did not have increased BCL2 protein, nor were the numbers of mature B cells or germinal centre B cells increased as would be expected if BCL2 was increased. Other lymphocyte subsets that are also regulated by BCL2 levels also displayed no difference in frequency in homozygousFbxo10mutant mice. These results support one of two conclusions: either FBXO10 does not regulate BCL2 in mice, or it does so redundantly with other ubiquitin ligase complexes. Possible candidates for the latter include FBXO11 or ARTS-XIAP. The difference between the role of FBXO10 in regulating BCL2 protein levels inC. elegansand in human DLBCL, relative to single-gene deficient mouse leukocytes, should be further investigated.
Exergy analysis of a three-spool turboprop engine during the flight of a cargo aircraft
Authors: Dinc Ali , Şöhret Yasin , Ekici Selcuk 
Published: August 2020

Purpose
This study aims to introduce exergy analysis of a three-spool turboprop engine during the complete flight.

Design/methodology/approach
In this study, a flight scenario of the aircraft is assumed. Operating parameters of the aircraft and its engine are modelled based on the assumed flight scenario with the aid of a genuine code. And then performance analysis of the engine is performed for each flight path point with the aid of exergy.

Findings
At the end of the study, major exergy parameters of the engine are calculated during the complete flight of a cargo aircraft three-spool turboprop engine.

Practical implications
Findings of the study may be beneficial for industry and practitioners to improve performance of the evaluated engine.

Originality/value
To the best of authors’ knowledge, this paper presented the exergy analysis of a three-spool turboprop engine during the complete flight for the first time. It was shown how the exergy destruction rate depends on the altitude and manoeuvre.
Agile social learning – capacity-building for sustainable development in higher education
Authors: Bolmsten Johan , Kitada Momoko 
Published: August 2020

Purpose
The purpose of this paper is to understand the usefulness of an agile social learning method in higher education to build capacity for sustainable development at the community level. Social learning methods intend to empower students (and instructors) to work together in connection with real-life issues – combined with acquiring a conceptual understanding – to analyze issues at hand and work out solutions. The agile format of the method was aimed at a subject that is adaptive and responsive to change to empower the students to take action toward sustainable development.

Design/methodology/approach
This study was based on a case study methodology where the running of the subject was documented and analyzed for two years. The target student group was maritime professionals who had an interest or were in a position to work with developing sustainable solutions in their home organizations (mostly in developing countries).

Findings
The results of the analysis indicate how the students learned about environmental, social and economic spheres of sustainable development and their linkages; how the subject format stimulated the students to develop different “learning paths” between the three spheres of sustainable development, which enabled a multi-faceted understanding of sustainable development issues; and, finally, how the students were able to design evolvable sustainable development solutions.

Originality/value
The results indicate both the novelty and usefulness of the agile social learning method to build capacity for sustainable development through the subject designed for higher education.
Diverse nitrogen cycling pathways across a marine oxygen gradient indicate nitrogen loss coupled to chemoautotrophic activity
Authors: Suter Elizabeth A. , Pachiadaki Maria G. , Montes Enrique , Edgcomb Virginia P. , Scranton Mary I. , Taylor Craig D. , Taylor Gordon T. 
Published: August 2020

SummaryGenetic markers and geochemical assays of microbial nitrogen cycling processes, including autotrophic and heterotrophic denitrification, anammox, ammonia oxidation, and nitrite oxidation, were examined across the oxycline, suboxic, and anoxic zones of the Cariaco Basin, Venezuela. Ammonia and nitrite oxidation genes were expressed through the entire gradient. Transcripts associated with autotrophic and heterotrophic denitrifiers were mostly confined to the suboxic zone and below but were also present in particles in the oxycline. Anammox genes and transcripts were detected over a narrow depth range near the bottom of the suboxic zone and coincided with secondary NO2− maxima and available NH4+. Dissolved inorganic nitrogen (DIN) amendment incubations and comparisons between our sampling campaigns suggested that denitrifier activity may be closely coupled with NO3− availability. Expression of denitrification genes at depths of high rates of chemoautotrophic carbon fixation and phylogenetic analyses of nitrogen cycling genes and transcripts indicated a diverse array of denitrifiers, including chemoautotrophs capable of using NO3− to oxidize reduced sulfur species. Thus, results suggest that the Cariaco Basin nitrogen cycle is influenced by autotrophic carbon cycling in addition to organic matter oxidation and anammox.
Genetic evaluation of heat tolerance in Holstein cows in Japan
Authors: Hagiya Koichi , Atagi Yamato , Osawa Takefumi , Yamazaki Takeshi 
Published: August 2020

AbstractWe used test‐day records and daily records from provincial weather stations in Japan to evaluate heat tolerance (HT) in Holstein cows according to a random regression test‐day model. Data were a total of 1,641,952 test‐day records for heritability estimates and 17,245,694 test‐day records for genetic evaluation of HT by using milk yield and somatic cell score (SCS) in Holstein cows that had calved for the first time in 2000 through 2015. Temperature–humidity index (THI) values were estimated by using average daily temperature and average daily relative humidity records from 60 provincial Japanese weather stations. The model contained herd–test‐day, with lactation curves on days in milk within month–age group as a fixed effect. General additive genetic effect and HT of additive genetic effect were included as random effects. The threshold value of THI was set to 60. For milk yield, estimated mean heritabilities were lower during heat stress (THI = 78; 0.20 and 0.28) than when below the heat stress threshold (THI ≤ 60; 0.26 and 0.31). For SCS, heritability estimates (range 0.08–0.10) were similar under all heat stress conditions. Genetic trends of HT indicated that EBVs of HT are changing in an undesirable direction.
How College Men Understand Unwanted Sex with Women
Authors: Ford Jessie V. , Maggio Christopher 
Published: August 2020

Recent studies have shown that heterosexual college men report unwanted sex at higher rates than one might expect. And yet, college men’s perceptions of unwanted sex with women have been studied infrequently. This study examines how men understand unwanted sex with women and the impact of these experiences. Thirty‐nine men, aged 18–25, were interviewed using open‐ended questions related to their unwanted sexual experiences. Our findings demonstrate how men frequently minimize unwanted sex by framing it as either funny, unimportant, or something that happens to women. However, we find evidence that men's attempts at minimization are not always successful and that some experiences are indeed upsetting and/or hurtful. Rather than treating all men as a homogeneous category, our analysis details the circumstances associated with more or less troubling experiences. We find that there are particular circumstances (e.g. incapacitation), as well as personal characteristics (e.g. virgin), that position men to be more distressed by unwanted sex. This heterogeneity of men’s experiences explains some, but not all, differences in effects. Our analysis reveals that in experiences described as mild to severe, men talk about unwanted sex in highly contradictory ways. We argue that these contradictions are evidence of broader cognitive and emotional work done by men to convince themselves that unwanted sex does not matter to them. Our findings raise the possibility that when men use hegemonic notions of masculinity to understand their unwanted experiences, these interpretations serve to minimize the emotional damage that men might otherwise report.
Ehrlichia canis infection in the cerebrospinal fluid of a dog characterized by morulae within monocytes and neutrophils
Authors: Lukács Robert M. , Peters Iain R. , Eminaga Salih , Buckeridge David M. 
Published: August 2020

AbstractAn 8‐year‐old neutered female English Pointer was referred to a veterinary referral center (southwest of England) with a 4‐5‐month history of fecal incontinence and no evidence of urinary incontinence. Blood and free‐catch urine samples were collected and sent to an off‐site laboratory. Further investigations were postponed until laboratory results were available. Blood results showed a mild leukopenia, mild nonregenerative anemia, moderate to marked thrombocytopenia, and a mild increase in ALT and ALP activities. The primary veterinarian and client did not proceed with any further investigations for thrombocytopenia. Three weeks after the initial presentation, there was considerable clinical deterioration and progression of neurologic signs. Thoracic radiographs and an abdominal ultrasonographic examination were unremarkable. Magnetic resonance imaging (MRI) of the brain and spinal cord revealed an intramedullary lesion at the level of the C7 vertebra, a cystic lesion in the forebrain, and a bilateral lesion in the thalamus. A lumbar cerebrospinal fluid (CSF) was collected. CSF analysis showed a robustly increased protein concentration and marked pleocytosis. The cytologic evaluation revealed a mixed cellular population. Occasional neutrophils and monocytoid cells showed purple spherical intracellular inclusions, resembling Ehrlichia morulae. An aliquot of CSF was used off‐label with a dot ELISA test, which showed a strong positive result for antibodies against Ehrlichia canis/Ehrlichia ewingii. PCR identified these morulae to be E canis. To best of the authors’ knowledge, this is the first case of ehrlichial infection in canine CSF where Ehrlichia sub‐species morulae present within neutrophils were confirmed to be Ehrlichia canis using PCR.
Design of Concentric Tube Robots Using Tube Patterning for Follow-The-Leader Deployment
Authors: Girerd Cédric , Schlinquer Thomas , Andreff Nicolas , Renaud Pierre , Rabenorosoa Kanty 
Published: August 2020

Abstract
Concentric tube robots (CTRs) have a great potential for use in medical applications. Coupled with a follow-the-leader (FTL) deployment, they allow navigation in constrained environments. However, they are subject to instabilities if one makes use of high curvatures for the tubes, long overlapping lengths of their curved sections, or long transmission lengths. One approach to improve their stability is to pattern the tubes of which they are composed by local removals of material along their lengths. Applying patterns on tubes was proved to be of interest for given deployed lengths of a CTR. In this article, we present a method to enlarge the application field of CTRs that deploy in a follow-the-leader manner, by integrating tube patterning in the design process, with a stability criterion. Our method allows the designer to determine a custom pattern geometry to theoretically ensure the stability of CTRs made of any number of constant curvature tubes, for a complete FTL deployment sequence, and while respecting a desired shape during deployment.
Towards Manufacturing Scale-Up of Air Retention Device for Colonoscopy
Authors: Cheng Pin Hao , Nelson Carl A. , Wolfe Joy 
Published: August 2020

Abstract
Colonoscopy is a very common diagnostic procedure and is considered relatively safe. Air insufflation is often used to increase the effectiveness of the procedure and decrease its duration. However, it is not uncommon for poor sphincter tone to cause loss of insufflation gas, thus complicating the workflow and lengthening the procedure. Air retention devices may address this shortcoming. In this paper we present an improved air retention device for colonoscopy and demonstrate its effectiveness through testing.
A Novel Transcatheter Edge-to-Edge Suturing Technique and Prototype for Repairing Tricuspid Valve Regurgitation
Authors: Zhingre Sanchez Jorge D. , Iaizzo Paul A. 
Published: August 2020

Abstract
Tricuspid valve regurgitation is a major clinical issue that continues to attract interest from interventional cardiologists and medical device designers due to its rising prevalence and progressive nature. This disease impact is exacerbated among the aging population, considered as high risk of mortality for open-heart surgical procedures. Furthermore, early intervention for tricuspid regurgitation following left-sided heart procedures continues to increase. Thus, percutaneous or transcatheter interventions have emerged as the new frontier for tricuspid valve therapy. Specifically, tricuspid leaflet plication, or edge-to-edge repair, is a valvular procedure to enhance the coaptation of the leaflets and reduce regurgitation. The current landscape of approved transcatheter devices for leaflet coaptation are exclusive to the mitral valve or being investigated for tricuspid treatment. However, most of these transcatheter systems are designed with high procedure specificities, are expensive, and require extensive procedural training. Hence, there is an opportunity to percutaneously plicate the tricuspid leaflets using commonly available right-heart catheter equipment. This study details a novel transcatheter repair procedure that can plicate the tricuspid valve leaflets solely using current market released catheters and/or surgical equipment. Testing and evaluation of this prototype procedure was performed using Visible Heart® methodologies.
Label Free Cell Purification Following Electroporation
Authors: Ringwelski Beth , Jayasooriya Vidura , Nawarathna Dharmakeerthi 
Published: August 2020

Abstract
Cell transfection by electroporation is a biological assay that has been utilized to inject exogenous molecules (e.g.: RNA, DNA and protein) into live cells. Recently, electroporation has been utilized in developing cell therapy for cancer (e.g., CAR T-cell). One of the major drawbacks in current electroporation methods is the cell death during the process. These dead cells can be detrimental, if injected back to the patients. Current cell filtering methods are unable purify T-cells following electroporation, this is due to the lack of unique biomarkers that target the apoptosis and necrosis of T-cells. To address this issue, we have developed a method using dielectrophoresis and microfluidics, where no prior labeling is needed to isolate dead cells from live cells. Upon electroporation, the cell sample has to be flowed through the microfluidic chip where a selective electric field is applied through specially designed electrodes so that the dead cells are trapped on the electrodes, and the live cells are able to flow through and are collected at the end. Results after purification of the cells using our method reveal that it is possible to achieve ∼100% of purity in filtering of the live cells. This method presents a viable solution to a critical concern regarding CAR T-cell manufacturing. This paper presents an extended study of the variation of efficacy in the design with the time from the electroporation.
Extended Reality Tools for Deep Brain Stimulation: A Framework and Design Concepts for Procedural Planning
Authors: Juhnke Bethany , Ahsan Muhammad , Rothweiler Paul , Erdman Arthur 
Published: August 2020

Abstract
Extended reality tools have been theorized to revolutionize medicine. Many tools on the market today fail to provide value in a clinical setting. Extended reality tools must emulate existing clinical workflows to add value to a doctor’s process. A framework is created to capture the clinical workflow by defining user tasks, graphical user interface functionalities and backend software functionalities. Two extended reality tool designs are presented based on the workflow captured in the framework. The first extended reality tool is designed for communication and collaboration, while the second tool is designed with a full immersive experience.
Patient Satisfaction in the Emergency Department Is Influenced by Total Length of Stay: An RFID Based Feasibility Study
Authors: Poigai Arunachalam Shivaram , Albertson Sabrina M. , Sir Mustafa Y. , Nestler David M. , Heaton Heather A. , Marisamy Gomathi , Pasupathy Kalyan S. 
Published: August 2020

Abstract
Real-time location systems (RTLS) such as radio frequency identification (RFID) have been shown to improve safety, save cost, and increase patient satisfaction in a healthcare setting especially in the emergency department (ED). Hospital administrators have realized the potential of these applications for improved workflow and operations and are positively adopting it despite the substantial implementation costs of such technologies. Our group has reported several studies with RFID data in the ED showing the relationship between RFID determined patient contact times and length of stay (LOS). In this study, we use ED RFID data to determine the relationship between the total LOS in ED to the overall patient satisfaction obtained from a survey report. ED survey results from 35 questions were obtained from patients after discharge. An observational cohort study was performed in the ED using RFID data from Jan 1 – Dec 31, 2017 matched with the ED survey participants. A total of 2595 survey data was used for logistic regression analysis with the RFID data. Results show that higher treatment LOS influenced overall patient satisfaction negatively. The results provide new insights into designing ideal patient-care team interactions to reduce overall LOS while providing optimal ED care, thereby improving patient satisfaction.
Comparison of Multiscale Frequency Characteristics of Normal Phonocardiogram With Diseased Heart States
Authors: Baala Sundaram Divaakar Siva , Kapoor Anoushka , Xie Jackie , Xu Natalie C. , Krishna Moorthy Prissha , Balasubramani Rogith , Shivaram Suganti , Muthyala Anjani , Poigai Arunachalam Shivaram 
Published: August 2020

Abstract
Phonocardiogram (PCG) signals are electrical recording of heart sounds containing vital information of diagnostic importance. Several signal processing methods exist to characterize PCG, however suffers in terms of sensitivity and specificity in accurately discriminating normal and abnormal heart sounds. Recently, a multiscale frequency (MSF) analysis of normal PCG was reported to characterize subtle frequency content changes in PCG which can aid in differentiating normal and abnormal heart sounds. In this work, it was hypothesized that MSF can discriminate normal PCG signal compared to an artifact, PCG with extra systolic heart sounds and murmur based on their varying frequency content. Various samples of PCG with normal and abnormal heart sounds were obtained from Peter Bentley Heart Sounds Database sampled at 44.1 kHz for analysis. The signal was filtered using a 4th order Butterworth lowpass filter with cutoff frequency at 200 Hz to remove higher frequency noise and MSF estimation was performed on the filtered dataset using custom MATLAB software. Mann-Whitney test was performed for statistical significance at p &lt; 0.05. Results indicate that MSF successfully discriminated normal and abnormal heart sounds, which can aid in PCG classification with more sophisticated analysis. Validation of this technique with larger dataset is required.
Dynamic Characteristics Analysis and FEM Modeling of Flexible Joints of an SMA-Activated Flexible Multi-Joint Needle
Authors: Karimi Saeed , Konh Bardia 
Published: August 2020

Abstract
Minimally invasive percutaneous needle-based procedures such as brachytherapy, ablation, and biopsy are standard clinical procedures in cancer interventions. Active needle steering increases the target placement accuracy, and consequently improves the clinical outcome. In this work, dynamical characteristic analysis and FEM modeling of flexible joints of a 3D steerable active flexible needle, when actuated by three Shape Memory Alloy (SMA) actuators, are studied. The Shape Memory Effect (SME) and Pseudoelasticity (PE) of the SMA actuators, their biocompatibility, and high corrosion resistance have made them appropriate alternatives in biomedical applications. Modelling the dynamics and FEM analyses of the flexible active needle during actuation is essential before predicting the active needle’s behavior inside tissue.
Sustainability in Railway Construction: LCA–LCC Based Assessment of Alternative Solutions for Track-Bed
Authors: Giunta Marinella , Bressi Sara , Losa Massimo 
Published: August 2020

Abstract
The economic and environmental sustainability of the Bitumen Stabilized Ballast (BSB) as construction and maintenance practice in railway track-bed is evaluated in comparison to the traditional ballast (TB). This aim is achieved integrating the results of an attributional Life Cycle Assessment (LCA), following a cradle-to-grave approach, and the Life Cycle Cost (LCC) analyses. The higher durability of BSB leads to arise environmental benefits in almost all the impact categories of LCA. Nevertheless, Bitumen Emulsion (BE) originates high level of impact on certain categories and they cannot be compensated by the reduction of the minor and major maintenance activities required by the BSB solution over the life cycle. The results of the LCA have been implemented in the LCC model for accounting the external costs due to the environmental impacts. From this analysis it emerges that the BSB technology, used since the construction stage and during the routine tamping, can provide economical savings.
Retracted: “Qualification of Track Parameters Based on a Review of Previous Studies” [2020 Joint Rail Conference, St. Louis, Missouri, USA, April 20–22, 2020, Conference Sponsors: Rail Transportation Division, ISBN: 978-0-7918-8358-7, Copyright © 2020 by ASME. Paper No. JRC2020-8065, pp. V001T08A007; 13 pages; doi: 10.1115/JRC2020-8065]
Authors: ASME  
Published: August 2020

Abstract
The above referenced paper has been removed from publication at the author’s request. October 2, 2020.
Copyright © 2020 by ASME