bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ageing and amyloidosis underlie the molecular and pathological alterations of tau in a mouse model of familial Alzheimer’s disease
Authors: Metaxas Athanasios , Thygesen Camilla , Kempf Stefan J. , Anzalone Marco , Vaitheeswaran Ramanan , Petersen Sussanne , Landau Anne M. , Audrain Hélène , Teeling Jessica L. , Darvesh Sultan , Brooks David J. , Larsen Martin R. , Finsen Bente 
Published: October 2019

AbstractDespite compelling evidence that the accumulation of amyloid-beta (Aβ) promotes neocortical MAPT (tau) aggregation in familial and idiopathic Alzheimer’s disease (AD), murine models of cerebral amyloidosis are not considered to develop tau-associated pathology. In the present study, we show that tau can accumulate spontaneously in aged transgenic APPswe/PS1ΔE9 mice. Tau pathology is abundant around Aβ deposits, and further characterized by accumulation of Gallyas and thioflavin-S-positive inclusions, which were detected in the APPswe/PS1ΔE9 brain at 18 months of age. Age-dependent increases in argyrophilia correlated positively with binding levels of the paired helical filament (PHF) tracer [18F]Flortaucipir, in all brain areas examined. Sarkosyl-insoluble PHFs were visualized by electron microscopy. Quantitative proteomics identified sequences of hyperphosphorylated and three-repeat tau in transgenic mice, along with signs of RNA missplicing, ribosomal dysregulation and disturbed energy metabolism. Tissue from the frontal gyrus of human subjects was used to validate these findings, revealing primarily quantitative differences between the tau pathology observed in AD patient vs. transgenic mouse tissue. As physiological levels of endogenous, ‘wild-type’ tau aggregate secondarily to Aβ in APPswe/PS1ΔE9 mice, this study suggests that amyloidosis is both necessary and sufficient to drive tauopathy in experimental models of familial AD.
Cannabidiol Regulates Gene Expression in Encephalitogenic T cells Using Histone Methylation and noncoding RNA during Experimental Autoimmune Encephalomyelitis
Authors: Yang Xiaoming , Bam Marpe , Nagarkatti Prakash S. , Nagarkatti Mitzi 
Published: October 2019

AbstractCannabidiol (CBD) has been shown by our laboratory to attenuate experimental autoimmune encephalomyelitis (EAE), an animal model of multiple sclerosis (MS). In this study, we used microarray and next generation sequencing (NGS)-based approaches to determine whether CBD would alter genome-wide histone modification and gene expression in MOG sensitized lymphocytes. We compared H3K4me3 and H3K27me3 marks in CD4+ T cells from naïve, EAE and CBD treated EAE mice by ChIP-seq. Although the overall methylation level of these two histone marks did not change significantly, the signal intensity and coverage differed in individual genes, suggesting that CBD may modulate gene expression by altering histone methylation. Further analysis showed that these histone methylation signals were differentially enriched in the binding sites of certain transcription factors, such as ZNF143 and FoxA1, suggesting that these transcription factors may play important roles in CBD mediated immune modulation. Using microarray analysis, we found that the expression pattern of many EAE-induced genes was reversed by CBD treatment which was consistent with its effect on attenuating the clinical symptoms of EAE. A unique finding of this study was that the expression of many miRNAs and lncRNAs was dramatically affected by CBD. In summary, this study demonstrates that CBD suppresses inflammation through multiple mechanisms, from histone methylation to miRNA to lncRNA.
Non-proteolytic calpain-6 interacts with VEGFA and promotes angiogenesis by increasing VEGF secretion
Authors: Oh Mijung , Rho Seung Bae , Son Chaeyeun , Park Kyoungsook , Song Sang Yong 
Published: October 2019

AbstractAngiogenesis is involved in both normal physiological and pathological conditions. Vascular endothelial growth factor (VEGF) is a major factor for promoting angiogenesis. The current anti-VEGF therapies have limited efficacy and significant adverse effects. To find novel targets of VEGFA for angiogenesis inhibition, we performed yeast two-hybrid screening and identified calpain-6 as a novel VEGFA-interaction partner and confirmed the endogenous VEGFA–calpain-6 interaction in mammalian placenta. A domain mapping study revealed that the Gly321–Asp500 domain in calpain-6 is required for the interaction with the C-terminus of the VEGFA protein. The functional significance of the VEGFA–calpain-6 interaction was explored by assessing its effect on angiogenesis in vitro. Whereas forced overexpression of calpain-6 increased the secretion of the VEGF protein and tube formation, knockdown of calpain-6 expression abrogated the calpain-6-mediated VEGF secretion and tube formation in HUVECs. Consistent with the domain mapping result, overexpressing calpain-6 without the VEGFA-interacting domain III (Gly321–Asp500) failed to increase the secretion of VEGF protein. Our results identify calpain-6, an unconventional non-proteolytic calpain, as a novel VEGFA-interacting protein and demonstrate that their interaction is necessary to enhance VEGF secretion. Thus, calpain-6 might be a potential molecular target for angiogenesis inhibition in many diseases.
Male and female cichlid fish show cognitive inhibitory control ability
Authors: Brandão Manuela Lombardi , Fernandes Ana Marina Tabah de Almeida , Gonçalves-de-Freitas Eliane 
Published: October 2019

AbstractInhibitory control is a way to infer cognitive flexibility in animals by inhibiting a behavioral propensity to obtain a reward. Here we tested whether there are differences in inhibitory control between females and males of the fish Nile tilapia owing to their distinct reproductive roles. Individuals were tested under a detour-reaching paradigm, consisting of training fish to feed behind an opaque barrier and, thereafter, testing them with a transparent one. Fish is expected to avoid trying to cross through the transparent barrier to achieve food (reward), thus showing inhibitory control by recovering the learned detour with the opaque apparatus. Both males and females learned to detour the transparent barrier with similar scores of correct responses, whereas females reached the food faster. This result is probably associated to their different sex roles in reproduction: females care for the eggs and fry inside their mouth (thus requiring a high inhibitory control not to swallow them), whereas males have to stay inside the territory defending it against intruder males, which also demands some inhibitory ability not to leave the spawning site and take the risk of losing it. Furthermore, this evidence of cognitive flexibility can enable social fish to deal with unpredictable interactions.
Effect of temperature on capping efficiency of zeolite and activated carbon under fabric mats for interrupting nutrient release from sediments
Authors: Hong Seung-Hee , Lee Jae-In , Lee Chang-Gu , Park Seong-Jik 
Published: October 2019

AbstractWe investigated the influence of temperature on the capping efficiency to interrupt the release of nutrients from lake sediments. A 3-cm layer of Zeolite (ZL) or activated carbon (AC) was placed on the contaminated sediments, and nonwoven fabric mats (NWFM) were placed on top of these capping materials. Laboratory incubation experiments were performed under three different temperatures, namely 4, 15, and 30 °C. Under the uncapped condition at 30 °C, dissolved oxygen (DO) was depleted after 30 days, while at 4 °C and 15 °C, DO was present until the end of this experiment. DO concentration in overlying water was more dependent on the temperature than capping condition. ZL/NWFM effectively blocked the release of N from the sediments, and the capping efficiencies of ZL/NWFM for NH4-N at 4, 15, and 30 °C were 98%, 96%, and 94%, respectively. For the interruption of P release, both ZL/NWFM and AC/NWFM were not effective at 4 and 15 °C. At 30 °C, however, AC/NWFM was effective, and its capping efficiencies at 30 °C for PO4-P and T-P were 74.0% and 79.9%, respectively. In summary, nutrient release from sediments was accelerated at higher temperatures, and the effect of capping was significant at high temperature.
Evaluation of different b-values in DWI and 1H MRS for pancreatic cancer and pancreatitis: a rabbit model
Authors: Hao Liguo , Liu Lijie , Meng Xin , Yu Guanghao , Li Enbang , Gu Hongqian 
Published: October 2019

Abstract
Pancreatic cancer is a common malignant tumor with high incidence of metastasis. Currently, there is no absolute standard for the choice of b-value for diffusion-weighted imaging (DWI) for pancreatic cancer. The b-value is rarely reported in animal model study, especially in pancreatic cancer/mass pancreatitis rabbit models. The authors’ aim was to determine the different b-values to differentiate the diagnosis of pancreatic cancer and mass pancreatitis in rabbit models using DWI. When comparing the effect of different b-values in diagnostic process, the pathological results could be regarded as the gold standard. In this research, 30 healthy New Zealand rabbits were selected and divided into three groups by random number table method: group 1 (pancreatic cancer), group 2 (mass pancreatitis) and the control group (healthy). After DWI (three different b-values 333, 667, 1000 s/mm2, respectively) and MRI examination, the model rabbits were then killed. Afterward, the tumor mass was removed for biopsy, and occupation anatomy and tumor histopathology were examined. Fat-suppressing sequences of T2WI, DWI, ADC, difference of ADC (DADC), and MRS were used. The present study determined that the effective differential diagnosis of pancreatic cancer and pancreatitis was determined at low b-values (333 s/mm2) when performed DWI inspection in rabbit models.
Homocysteine and psoriasis
Authors: Lin Xiran , Meng Xianmin , Song Zhiqi 
Published: October 2019

AbstractPsoriasis is caused by a complex interplay among the immune system, genetic background, autoantigens, and environmental factors. Recent studies have demonstrated that patients with psoriasis have a significantly higher serum homocysteine (Hcy) level and a higher prevalence of hyperhomocysteinaemia (HHcy). Insufficiency of folic acid and vitamin B12 can be a cause of HHcy in psoriasis. Hcy may promote the immuno-inflammatory process in the pathogenesis of psoriasis by activating Th1 and Th17 cells and neutrophils, while suppressing regulatory T cells. Moreover, Hcy can drive the immuno-inflammatory process by enhancing the production of the pro-inflammatory cytokines in related to psoriasis. Hcy can induce nuclear factor kappa B activation, which is critical in the immunopathogenesis of psoriasis. There may be a link between the oxidative stress state in psoriasis and the effect of HHcy. Hydrogen sulfide (H2S) may play a protective role in the pathogenesis of psoriasis and the deficiency of H2S in psoriasis may be caused by HHcy. As the role of Hcy in the pathogenesis of psoriasis is most likely established, Hcy can be a potential therapeutic target for the treatment of psoriasis. Systemic folinate calcium, a folic acid derivative, and topical vitamin B12 have found to be effective in treating psoriasis.
Multifaceted roles of porin in mitochondrial protein and lipid transport
Authors: Endo Toshiya , Sakaue Haruka 
Published: October 2019

Abstract
Mitochondria are essential eukaryotic organelles responsible for primary cellular energy production. Biogenesis, maintenance, and functions of mitochondria require correct assembly of resident proteins and lipids, which require their transport into and within mitochondria. Mitochondrial normal functions also require an exchange of small metabolites between the cytosol and mitochondria, which is primarily mediated by a metabolite channel of the outer membrane (OM) called porin or voltage-dependent anion channel. Here, we describe recently revealed novel roles of porin in the mitochondrial protein and lipid transport. First, porin regulates the formation of the mitochondrial protein import gate in the OM, the translocase of the outer membrane (TOM) complex, and its dynamic exchange between the major form of a trimer and the minor form of a dimer. The TOM complex dimer lacks a core subunit Tom22 and mediates the import of a subset of mitochondrial proteins while the TOM complex trimer facilitates the import of most other mitochondrial proteins. Second, porin interacts with both a translocating inner membrane (IM) protein like a carrier protein accumulated at the small TIM chaperones in the intermembrane space and the TIM22 complex, a downstream translocator in the IM for the carrier protein import. Porin thereby facilitates the efficient transfer of carrier proteins to the IM during their import. Third, porin facilitates the transfer of lipids between the OM and IM and promotes a back-up pathway for the cardiolipin synthesis in mitochondria. Thus, porin has roles more than the metabolite transport in the protein and lipid transport into and within mitochondria, which is likely conserved from yeast to human.
Calibration technology and application of laser power meter
Authors: Xu Bai 
Published: October 2019

Based on laser power meter calibration requirements, through the purchase of standard equipment and field monitoring equipment developed, standard laser detector, optical path related equipment, such as construction of laser radiation power meter calibration test system, implementation of laser power meter of laboratory test and vehicle, large equipment form a complete set of laser power meter field test and field test of the radiated power of the laser equipment. Calibration system is introduced in detail the composition and working principle of the uncertainty of the system are analysed in detail, and through the system stability test, laser power, beam monitoring and correction device performance test, ate test for power meter calibration experiment data analysis, proved in this study, the laser power meter calibration method provided will enable calibration system, the expanded uncertainty of relative reached 2% (k  = 2), which can realise wavelength of 532 and 1064 nm, power measuring the range of 0.1 mW–20 W laser power meter calibration.
Prävalenz von leichten kognitiven Störungen und Demenzen in der ambulanten Routineversorgung in Deutschland 2009–2016
Authors: Bohlken Jens , von Stillfried Dominik , Schulz Mandy 
Published: October 2019

Zusammenfassung
Ziel In Deutschland wird ein Anwachsen der Patienten mit Demenz von 1,7 Mio. (2017) auf über 3 Mio. (2050) erwartet. Präventive Maßnahmen und Therapien zielen auf frühe Phasen der Demenzerkrankung, wie der leichten kognitiven Störung (MCI). Die Behandlungsprävalenzen für MCI und Demenz der Jahre 2009 – 2016 werden vergleichend dargestellt.
Methodik Datengrundlage bildeten die bundesweiten vertragsärztlichen Abrechnungsdaten gemäß § 295 SGB V. Für die Jahre 2009–2016 wurden in Hausarzt- und Facharztpraxen behandelte prävalente Patienten mit leichten kognitiven Störungen (PmMCI) und Demenz (PmD) identifiziert.
Ergebnisse Die Anzahl prävalenter PmMCI/PmD wuchs von 50 760/1 014 381 (2009) auf 166 919/1 416 319 (2016) und die entsprechende Behandlungsprävalenzrate von 0,13 % auf 0,42 % (MCI) bzw. von 2,52 % auf 3,55 % (Demenz).
Schlussfolgerung Trotz des deutlichen Zuwachses bleibt die Behandlungsprävalenz der abrechnungsrelevant dokumentierten PmMCI weit unter der geschätzten bevölkerungsbezogenen Anzahl von 2,8–3,7 Mio. prävalenter PmMCI. In den nächsten Jahren ist daher mit einer weiteren Zunahme der PmMCI im ambulanten Versorgungsbereich zu rechnen.
Versorgungserwartungen von Patienten mit leichten kognitiven Störungen in einer Facharztpraxis
Authors: Bohlken Jens , Rädke Anika , Kohlmann Thomas , Riedel-Heller Steffi , Rapp Michael 
Published: October 2019

Zusammenfassung
Ziel Beschreibung von Versorgungserwartungen von Patienten mit leichten kognitiven Störungen (PmMCI).
Methoden Befragung von 51 PmMCI in einer fachärztlichen Versorgerpraxis. Fragebogen in Anlehnung an die CANE-Beurteilungsskala. Kontrollvariablen u. a. MMST und GDS.
Ergebnisse 35 % bzw 8 % erlebten die Gedächtnisstörungen als mittelschwer bzw. schwerwiegend und 27 % bzw. 8 % fühlten sich im Alltag häufig bzw. andauernd beeinträchtigt. Über 70 % waren trotz der Gedächtnisprobleme ausgeglichener Stimmung. Arztbezogene Erwartungen: Verlaufskontrolle (96 %), Demenz-Risikoabschätzung (73 %), Gedächtnistraining (55 %), medikamentöse Behandlung (33 %). Medikamentenbezogene Erwartungen: spürbare Wirkung (41 %), Bereitschaft, Nebenwirkungen zu ertragen (29 %), Bevorzugung pflanzlicher Medikation (59 %). Nichtmedikamentöse Therapieerwartungen: sportliche Aktivierung (63 %), Gedächtnistraining (51 %) und Ernährungsberatung (37 %). Der Wunsch nach psychotherapeutischen Gesprächen (27 %) war mit höheren Depressionswerten der GDS assoziiert.
Schlussfolgerung PmMCI erlebten Gedächtnisstörungen als Belastung und Beeinträchtigung und erwarteten diagnostische und therapeutische Maßnahmen. PmMCI mit depressiver Symptomatik erwarteten zusätzlich psychotherapeutische Gespräche.
Synthesis of Nonsymmetric Iminophosphonamines by Kirsanov Condensation
Authors: Peganova Tatyana A. , Kalsin Alexander M. 
Published: October 2019

Despite the growing interest in iminophosphonamines R2P(NHR′)(NR′), nonsymmetric examples bearing different N,N′-substituents are quite rare and have been prepared exclusively by the Staudinger reaction. We report here the synthesis of a series of new iminophosphonamines Ph2P(NHR)(NR′) (R = Me, t-Bu, o-Tol; R′ = p-Tol, o-Tol, 2,6-Xyl, 2,6-Diip, p-Ts) showing that the Kirsanov condensation is a viable and simpler approach, although with some limitations. This method allows the synthesis to be accomplished in a one-pot manner via stepwise double amination of a trihalophosphorane and permits the introduction of at least one sterically bulky N-substituent. The second amination step is shown to be highly sensitive to: (a) the steric bulk of the amine, and (b) the acidity of the aminohalophosphonium intermediate.
Ablation of Intrahepatic Cholangiocarcinoma
Authors: Sweeney Jennifer , Parikh Nainesh , El-Haddad Ghassan , Kis Bela 
Published: October 2019

AbstractIntrahepatic cholangiocarcinoma is the second most common primary liver cancer but represents only a small portion of all primary liver cancers. At the time of diagnosis, patients are often not surgical candidates due to tumor burden of other comorbidities. In addition, there is a very high rate of tumor recurrence after resection. Local regional therapies, specifically ablative therapies of radiofrequency ablation, microwave ablation, cryoablation, and irreversible electroporation, have proven to be beneficial with other hepatic tumors. The purpose of this review is to provide an overview and update of the medical literature demonstrating ablative therapy as a treatment option for intrahepatic cholangiocarcinoma.
Ablation plus Transarterial Embolic Therapy for Hepatocellular Carcinoma Larger than 3 cm: Science, Evidence, and Future Directions
Authors: Lewis Andrew R. , Padula Carlos A. , McKinney J Mark , Toskich Beau B. 
Published: October 2019

AbstractThermal ablation is widely regarded as definitive therapy for early-stage hepatocellular carcinoma, but its efficacy decreases in tumors greater than 3 cm. Extensive clinical studies have supported improved outcomes provided through combining transarterial embolic therapy with ablation in the treatment of larger tumors. This article will provide a survey of the science and data for combination therapy in both thermal and nonthermal ablation modalities, as well as describe emerging applications.
The Determinants of M-Health Adoption in Developing Countries: An Empirical Investigation
Authors: Alaiad Ahmad , Alsharo Mohammad , Alnsour Yazan 
Published: October 2019

Abstract
Background The potential benefit of mobile health (M-Health) in developing countries for improving the efficiency of health care service delivery, health care quality, and patient safety, as well as reducing cost, has been increasingly recognized and emphasized in the last few years.
Objective Limited research has investigated the facilitators and barriers for the adoption of M-Health in developing countries to secure successful implementation of the technology. To fill this knowledge gap, we propose an integrative model that explains the patient's adoption behavior of M-Health in developing countries grounded on the unified theory of acceptance and use of technology, dual-factor model, and health belief model.
Method We empirically tested and evaluated the model based on data collected using a survey method from 280 patients living in a developing country. Partial least squares (PLS-SEM) technique was used for data analysis.
Results The results showed that performance expectancy, effort expectancy, social influence, perceived health threat, M-Health app quality, and life quality expectancy have a direct positive effect on patients’ intention to use M-Health. The results also showed that security and privacy risks have a direct negative effect on the patient's intention to use M-Health. However, resistance to change was found to have an indirect negative effect on patients’ intention to use M-Health through the performance expectancy.
Conclusion The research contributes to the existing literature of health information systems and M-Health by better understanding how technological, social, and functional factors are associated with digital health applications and services use and success in the context of developing countries. With the widespread availability of mobile technologies and services and the growing demand for M-Health apps, this research can help guide the development of the next generation of M-Health apps with a focus on the needs of patients in developing countries. The research has several theoretical and practical implications for the health care industry, government, policy makers, and technology developers and designers.
The Role of Neurophysiological Monitoring during Complex Aneurysm Surgery: Report of Two Cases and Review of the Literature
Authors: Brock Roger Schmidt , Gomes Marcos Queiroz Teles , Pahl Félix Hendrik , Dal Col Lúcio José Erasmo , Pereira Júnior José Francisco , Nascimento Clarissa Gambarra Nóbrega , Martins Henrique Oliveira , Oliveira Matheus Fernandes 
Published: October 2019

AbstractThe surgical treatment of intracranial aneurysms is a routine operation in the neurosurgeon practice. Complex aneurysms are those with morphological irregularities, usually large or giant; thrombosed, partially thrombosed or calcified; with aberrant fundus/neck ratio and near eloquent neurological structures. These cases demand special skills by the surgical team. The present article is a report of two cases of complex aneurysms successfully treated, with a discussion on the role of neurophysiological monitoring. In these two cases of supra- and infratentorial complex giant aneurysms, intraoperative monitoring was extremely relevant. Thus, we believe that treating complex and giant aneurysms carries several pitfalls, and the use of multimodal intraoperative monitoring is mandatory to mitigate risks and deliver the best result to the patient.