bluuebunny/crossref_metadata_2025_split huggingface dataset:
Middle-aged Lebanese women interpretation of sexual difficulties: A qualitative inquiry
Authors: Azar Mathilde , Bradbury-Jones Caroline , Kroll Thilo 
Published: April 2020

Abstract
Background : The study explores women’s perception and experience of sexual difficulties and how they are shaped by the male-centred sexual interactions and social norms. The study is part of a work programme guided by a qualitative research design to provide a comprehensive understanding of women’s sexuality. The need to address the subject was triggered by the high prevalence of female sexual problems that are defined based on the standards of sexual function and dysfunction. The multidimensional nature of these problems could not be limited to one single definition. Yet, there is a scarcity of research that reflects on women’s subjective views on sexual difficulties. This is particularly crucial at the middle-age where women undergo hormonal and psychosocial changes that may affect their sexual life. Methods : Using in-depth individual and focus groups interviews, 52 Lebanese women aged 40-55 years discussed their thoughts, feelings and behaviours concerning sexual difficulties. In respect to ethical principles, women were recruited purposefully from clinical and non-clinical settings to get maximum sampling variation that provided rich information and deep understanding of the subject. Recordings were transcribed verbatim and analysed in reference to the framework analysis. Many strategies were adopted to ensure rigour. Results : Women’s narratives led to four themes that provided a comprehensive understanding of sexual difficulties. These are women’s inability to communicate sexual desires and concerns; male-related sexual difficulties; marital conflicts; and sexual difficulties as context bound. Women’s sexual difficulties are driven by double standards and inhibiting sexual socialization in addition to relational and psychosocial burdens. Conclusion : The study makes a unique contribution in voicing women’s views and concerns as sexuality is underreported and poorly researched in Lebanon. It shed light on many aspects of female sexual difficulties that are multifaceted and contextually bound. Findings have implications on education, clinical practice and research. It is particularly important to provide Lebanese women with a culturally sensitive professional assistance, empower them to recognise their sexual-self and get their needs met. More qualitative research about women’s sexual difficulties is warranted to enrich the body of knowledge and promote women’s sexual health and overall wellbeing.
Time correlation between serial RT-PCR results and serial chest CT imaging and serial CT changes of Coronavirus 2019 (COVID-19) Pneumonia: 155 cases study from China
Authors: Gu Jinfeng , Yang Li , Li Tao , Liu Ye , Zhang Jing , Ning Kejia , Su Dajian 
Published: April 2020

Abstract
Objective: To determine the CT role in the early detection of COVID-19 infection and serial CT changes in disease course in the patients with COVID-19 pneumonia. Methods: From January 21 to February 18, 2020, all the patients who were suspected of novel coronavirus infection and verified by RT-PCR test were retrospectively enrolled in our study. All the patients underwent serial RT-PCR tests and serial CT imaging. The time correlation between serial RT-PCR results (negative conversion to positive, positive to negative) and serial CT imaging was investigated, and serial CT changes were evaluated. Results: One hundred and fifty-five patients with confirmed COVID-19 pneumonia were evaluated. The time of chest CT detection of COVID-19 pneumonia was 2.61 days earlier than RT-PCR test (p=0.000). The time of lung CT improvement was significantly shorter than that of RT-PCR conversion to negative (p=0.000). Three stages were identified from the onset of the initial symptoms: Stage 1 (0-3 days); Stage 2 (4-7 days); and stage 3 (8-14 days and later). Ground glass opacity (GGO) was predominant on stage 1, then consolidation and crazy paving sign were dramatically increased on stage 2. On stage 3, fibrotic lesion was growing largely. There was significant difference for the main CT features (p=0.000), the number of involved lobes (p=0.001), and lesion distribution ( p=0.000) among different stages. Conclusion: Chest CT was earlier to detect COVID-19 pneumonia compared to RT-PCR results and monitor disease course. Combined imaging features with epidemiology history and clinical information could facilitate early diagnosis of COVID-19 pneumonia.
Radiation resistance of novel SARS-CoV-2: A Monte Carlo investigation on characterization and determination of gamma-ray attenuation properties
Authors: Tekin H.O. , Kara U. , Issa Shams A.M. , Inanc Ibrahim , Kilicoglu O. , Elshami Wiam , Aslan Ismail , Susoy G. , Kurt Ahmet Arif , Konuk Muhsin , Ayseli Mehmet Turan 
Published: April 2020

Abstract
In late December 2019, the new viral pneumonia outbreak was first detected in Wuhan, the largest metropolitan area in China's Hubei province. The 2019–20 coronavirus pandemic is an ongoing pandemic, caused by the severe acute respiratory syndrome-2 (SARS-CoV-2), was named as Coronavirus disease 2019 (COVID-19) by World Health Organization (WHO). It is well known that radiation can cause mutations in bacteria and viruses. Therefore, characterization of the radiation resistance and interaction properties of viruses provides the opportunity in terms of risk assessment and future aspects. In this study, 3 types of viruses (SARS-CoV-CAS Number 587886-51-9, Influenza-CAS Number 141368-69-6 and SARS-CoV-2 GlycoProtein 6VSB.) were modeled with the Monte Carlo simulation method (MCNPX version 2.6.0). The vital radiation attenuation properties such as linear attenuation coefficients, energy absorption buildup factors (EABF), exposure buildup factors (EBF), relative dose distributions (RDD) were examined using advanced simulation methods. Moreover, the spike protein of SARS-CoV-2 is modelled from the structures in the Protein Data Bank. As a result of the study, we could say that the most radiation resistance was observed in SARS-CoV when compared with Influenza and Covid-19. It could be one of the reasons for SARS-CoV’s resistance to mutation from its outbreak time. On the other hand, Covid-19 is more resistant to radiation than Influenza. Therefore it could be expected that Covid-19 would have the similar behaviors against ionizing radiation as Influenza has.
First report of molecular characterization and phylogeny of Trichuris fossor Hall, 1916 (Nematoda: Trichuridae)
Authors: Hughes Malorri R. , Duffield Deborah A. , Howe Dana K. , Denver Dee R. 
Published: April 2020

Abstract
Because species of Trichuris are morphologically similar and ranges of host preference are variable, using molecular data to evaluate species delineations is essential for properly quantifying biodiversity of and relationships within Trichuridae. Trichuris fossor has been reported from Thomomys spp. (Rodentia: Geomyidae, ‘pocket gophers’) hosts based on morphological features alone. Partial 18S rRNA sequences for specimens identified as T. fossor based on morphology, along with sequences from 26 additional taxa, were used for a phylogenetic analysis. Evolutionary histories were constructed using maximum likelihood and Bayesian inference. In both analyses, the specimens fell within the Trichuris clade with 100% support and formed a distinct subclade with 100% support. These results confirm that T. fossor is a distinct species and represent the first molecular report for it. Relatedness among species within the family were well resolved in the BI tree. This study represents an initial effort to obtain a more comprehensive view of Trichuridae by including a new clade member, T. fossor. A better understanding of Trichuridae phylogeny could contribute to further characterization of host-associations, including species that infect livestock and humans.
Differences in parasitism of root-knot nematodes (Meloidogyne spp.) on oilseed radish and oat
Authors: Hamidi Negin , Hajihassani Abolfazl 
Published: April 2020

Abstract
Oilseed radish and oat are cool season annual crops that are potentially used as “trap” or “biofumigant” crops for the suppression of plant-parasitic nematodes in soil. Cultivars of oilseed radish (Carwoodi, Cardinal, Final, Image, Concorde, Control, Eco-Till, Karakter and Cannavaro), white (Tachiibuki) and black (Pratex) oats were evaluated for their ability to reduce reproduction of three root-knot nematode species: Meloidogyne javanica, M. incognita race 3, and M. arenaria race 1. Nematode penetration and development were also evaluated using selected resistant and susceptible cultivars under greenhouse conditions. Root galling severity, number of eggs per gram of fresh root, and rate of reproduction varied among the cultivars in response to nematode infection. Oilseed radish cv. Carwoodi was resistant to M. javanica, whereas Karakter and Concorde were maintenance hosts allowing the nematode to maintain or increase its population on the plants. For M. incognita, Control and Carwoodi oilseed radish and Tachiibuki oat were resistant hosts. The cultivars that supported little reproduction of M. arenaria were Karakter and Carwoodi radish, and Tachiibuki oat. Comparable numbers of nematodes entered the roots of susceptible and resistant cultivars of oilseed radish and oat during early stages of infection. However, the development of the nematodes as evident from counting young and egg-laying females in roots were significantly decreased or inhibited in the resistant cultivars compared to the susceptible cultivars indicating that resistance occurs at post-infection stages. Histopathological examinations of galled-root tissues also revealed the susceptibility and resistance responses of selected cultivars of oilseed radish and oat to these nematode species.
Facile preparation of 3D GO with caffeic acid for efficient adsorption of norfloxacin and ketoprofen
Authors: Lu Ying , Li Youlin , Gao Yi , Ai BoXuan , Gao Wei , Peng Guilong 
Published: April 2020

Abstract
In this paper, a simple and green method was developed to fabricate a three-dimensional (3D) graphene-based material with the assistance of caffeic acid (CA). The prepared 3D graphene displayed fast and high sorption for norfloxacin (NOR) and ketoprofen (KP). Their adsorption equilibrium was achieved within 12 h for NOR and KP, which was attributed to their fast diffusion in the porous structure of the 3D graphene. The maximum adsorbed amount of this adsorbent was 220.99 mg/g for NOR and 125.37 mg/g for KP according to the Langmuir model at pH 6.6, 298 K. In the competitive adsorption of six pharmaceuticals, the organic compounds in the form of cations are preferentially adsorbed on the adsorbent. The co-existing organic compounds in the actual wastewater do not seriously inhibit the adsorption of NOR and KP. This study provides the theoretical basis for the facile and low-cost preparation of high-performance 3D graphene adsorbents. The results of this study demonstrate the potential utility of 3D graphene as a very effective adsorbent for pharmaceuticals removal from contaminated water.
Biochemical parameters and 18S rRNA gene sequence analysis amongst green microalgal strains from selected aquatic sites of Eastern India
Authors: Vishwakarma Rashi , Dhar Dolly Wattal , Jena Mrutyunjay , Shukla Madhulika 
Published: April 2020

AbstractIn the present study, 24 green microalgae strains were isolated from selected aquatic sites of India. These were microscopically identified as Chlamydomonas sp., Scenedesmus sp., Chlorella sp., Dictyosphaerium sp. and Dunaliella sp. Nannochloropsis sp. (MCC 25), was used as a reference strain. Results showed that Dictyosphaerium sp. (MCC 10 and MCC 12) showed relatively higher nutritive content. The total soluble proteins in the reference strain was 21.4%, whereas it showed carbohydrate content of 17.2% and the lipids were 3.4% on a dry weight basis. Best performing strains were identified by biochemical characterization. Five genera were selected for molecular identification since they were the most representative based upon their area of isolation and their optimum content of total soluble proteins, carbohydrates and lipids. 18S rRNA sequencing authenticated their identification as Scenedesmus sp., Dictyosphaerium sp. and Chlorella sp. The sequences of these have been submitted in NCBI database with accession numbers as KT808247–KT808251. The correlation matrix showed positive correlation between carbohydrates and lipids, while negative correlation was seen between proteins and carbohydrates and between proteins and lipids. This study emphasizes the need for complete compositional analysis of the biomass for the possible applicability in the area of value addition.
Helicobacter suis Infection in Mouse Induced not Only Gastric, but Hepatic and Pulmonary MALT Lymphoma: Relation to Substance P
Authors: Nakamura Masahiko , Kodama Yosuke , Øverby Anders , Takahashi Shinichi , Ohshima Koichi , Suzuki Hidekazu , Murayama Somay Y. , Matsui Hidenori 
Published: April 2020

Background:
The hepatic and pulmonary MALT lymphoma (mucosa-associated lymphoid tissue
lymphoma) is clinically occasionally observed but its pathogenesis is unknown and thought to be important to
establish the treatment strategy.

Objectives:
The present study was designed to clarify the characteristics of these lymphomas and the effect of the
Helicobacter eradication regimen and substance P antagonist.

Methods:
After the long term infection of Helicobacter suis to the C57BL/6 mice stomach, the whole organ was
surveyed pathologically. Histochemical characteristics of the lesion and the localization of bacteria were observed.
In addition, the effect of the administration of antibiotics and a proton pump inhibitor or the substance P
antagonist was investigated.

Results:
We have detected the hepatic and pulmonary MALT lymphoma after the long term infection. In situ
hybridization study revealed the positive reaction of Helicobacter suis in the hepatic and pulmonary MALT lymphoma.
After the administration of antibiotics and a proton pump inhibitor, the bacterial number has significantly
decreased and the tumor size in the fundus, liver and lung markedly reduced. Substance P immunoreactivity was
clearly shown in the lymphoma cells in the liver and lung, and the spantide II administration induced the marked
decrease in the size of tumors.

Conclusion:
By our experiments using the long term infection of Helicobacter suis to the C57BL/6 mice, we
have detected the liver and pulmonary MALT lymphoma. In situ hybridization study suggested the direct interaction
of this bacterium to the etiology of these lesions. Substance P within the lymphoma cells was suggested to
work on the maintenance of the extragastric MALT lymphoma.
Systematic Literature Review on strengthening Eye Care Follow-Up Among Glaucoma Patients in Limpopo Province
Authors: Tshivhase Shonisani 
Published: April 2020

Background:
Adherence to prescribed glaucoma medications is often poor and proper adherence can be challenging for most patients

Objective:

The purpose of this systematic literature review is to identify and evaluate studies that have tested the impact of each intervention on glaucoma adherence based on their quality outcome measure.

Methods:
A comprehensive search of database was conducted from January 2009 to January 2019. We systematically reviewed the literature and identified sixteen studies that used educational interventions to improve glaucoma medication adherence. Eleven out of 16 eligible studies were subjected to Randomized Controlled Trial (RCTs) and the remaining four were reviewed as observational studies. One study was reviewed through both observational plus randomized control trial method.

Results:
Out of the eleven (68.75%) RCTs interventions done, five (31.25%) showed improvement in medication adherence and persistence with eye drop instillation, whereas (n=6) did not show any significant improvement on their medication adherence. The quality of each study was evaluated using the Jadad score calculation and the Ottawa-Newcastle.

Conclusion:

Using information from this systematic review and Health Behavior Model, we created a theoretical framework to illustrate how counseling and education can improve medication adherence amongst glaucoma patients in the country.
Meta-Analysis of Expression of the Stress Tolerance Associated Genes and Uncover their Cis-Regulatory Elements in Rice (Oryza sativa L.)
Authors: Shariatipour Nikwan , Heidari Bahram 
Published: April 2020

Background:
Rice contributes to the staple food of more than half of the world’s population. However, its productivity is influenced by various biotic and abiotic stresses. Genetic engineering and plant breeding tools help to overcome the adverse effects of environmental stresses. The advanced bioinformatics tools provide information for a better understanding of the mechanisms underlying stress tolerance, gene expression profiles and functions of the important genes and cis-regulatory elements involved in better performance under abiotic stresses.

Objective:
To identify the key genes involved in the tolerance mechanism for abiotic stresses and their regulatory networks in rice (Oryza sativa L.).

Methods:
A total of 152 various microarray datasets associated with nine rice trials were retrieved for expression meta-analysis through various bioinformatics tools.

Results:
The results indicated that 29593, 202798, 73224 and 25241 genes represented significant differential expression under cold, drought, salinity and heat stress conditions compared with the control condition, respectively. Twenty three highly overexpressed genes were identified under the evaluated abiotic stresses. The transcription regulatory activity of differentially expressed genes was mainly due to hormone, light and stress-responsive cis-acting regulatory elements among which ABRE, ARE, CGTCA-motif, GARE-motif, TGACG-motif, G-box, G-Box, GAG-motif, GA-motif, TCT-motif, Box 4, Sp1, HSE, MBS and TC-rich repeats were the most important in the promoter sites of the identified up-regulated genes. The results of cis-acting regulatory analysis suggest that 15 cis-acting regulatory elements were contributed to the tolerance mechanisms for abiotic stresses.

Conclusion:
The result of expression meta-analysis in this study provides an insight for plant breeders for better understanding the function of the genes and their regulatory mechanism in plants (especially cereals) exposed to different abiotic stresses. The outcome of this study suggests practical approaches for designing unified breeding programmes to breed multi-abiotic stress-tolerant species.
Talin-1 Gene Expression as a Tumor Marker in Hepatocellular Carcinoma Patients: A Pilot Study
Authors: Mohamed Amal A. , El-Toukhy Naglaa , Ghaith Doaa M. , Badawy Ingy , Abdo Sara M. , Elkadeem Mahmoud , Mahrous Mohamed N. , Abd-Elsalam Sherief 
Published: April 2020

Background & Aims:
Hepatocellular Carcinoma (HCC) is the most common primary liver tumor. It is the second most common cancer in men and the sixth in women in Egypt. One of the proteins participating in the trans-endothelial migration is Talin-1. It also has a role in the formation and metastasis of different types of cancer. This study aimed to evaluate the diagnostic impact of Talin-1 gene expression in HCC Egyptian patients.

Methods:
Our study included forty HCC patients, thirty liver cirrhosis patients without HCC and thirty healthy subjects. For all groups, clinical and biochemical parameters were investigated. Tumor characteristics were assessed and tumor staging was done using Okuda, CLIP, VISUM and Tokyo staging systems. In addition, Serum Alpha-Fetoprotein (AFP) levels were assayed using Enzyme Immunoassay (EIA) and Talin-1 gene expression was assessed in the Peripheral Blood Mononuclear Cells (PBMCs) via quantitative real-time Polymerase Chain Reaction (PCR).

Results:
Talin-1 gene expression was significantly upregulated in HCC patients in comparison to cirrhotic and control subjects. The Receiver Operating Characteristic (ROC) analysis indicated that Talin-1 gene expression surpasses serum levels of AFP in the diagnosis of HCC. In particular, the cut off value of 9.5 (2-∆∆Ct) recorded an AUC of 85.7% with a sensitivity of 93.3% and specificity of 80%.

Conclusion:
Our data confirmed an évident diagnostic role of Talin-1 gene expression for HCC detection.
Comparison of Social Media, Syndromic Surveillance, and Microbiologic Acute Respiratory Infection Data: Observational Study
Authors: Daughton Ashlynn R , Chunara Rumi , Paul Michael J 
Published: April 2020

BackgroundInternet data can be used to improve infectious disease models. However, the representativeness and individual-level validity of internet-derived measures are largely unexplored as this requires ground truth data for study.ObjectiveThis study sought to identify relationships between Web-based behaviors and/or conversation topics and health status using a ground truth, survey-based dataset.MethodsThis study leveraged a unique dataset of self-reported surveys, microbiological laboratory tests, and social media data from the same individuals toward understanding the validity of individual-level constructs pertaining to influenza-like illness in social media data. Logistic regression models were used to identify illness in Twitter posts using user posting behaviors and topic model features extracted from users’ tweets.ResultsOf 396 original study participants, only 81 met the inclusion criteria for this study. Of these participants’ tweets, we identified only two instances that were related to health and occurred within 2 weeks (before or after) of a survey indicating symptoms. It was not possible to predict when participants reported symptoms using features derived from topic models (area under the curve [AUC]=0.51; P=.38), though it was possible using behavior features, albeit with a very small effect size (AUC=0.53; P≤.001). Individual symptoms were also generally not predictable either. The study sample and a random sample from Twitter are predictably different on held-out data (AUC=0.67; P≤.001), meaning that the content posted by people who participated in this study was predictably different from that posted by random Twitter users. Individuals in the random sample and the GoViral sample used Twitter with similar frequencies (similar @ mentions, number of tweets, and number of retweets; AUC=0.50; P=.19).ConclusionsTo our knowledge, this is the first instance of an attempt to use a ground truth dataset to validate infectious disease observations in social media data. The lack of signal, the lack of predictability among behaviors or topics, and the demonstrated volunteer bias in the study population are important findings for the large and growing body of disease surveillance using internet-sourced data.
Sistema de pensiones en México. Una burbuja a punto de estallar
Authors: Alanis Gutiérrez Teresa Lizeth , Soto Esquivel Roberto 
Published: April 2020

<p>Las últimas tres décadas se han caracterizado por la hegemonía neoliberal y la profundización del proceso de globalización financiera, provocando inestabilidad y profundas crisis financieras y productivas que influyen en el aumento del desempleo, desigualdad, informalidad y precariedad laboral. A su vez, las continuas reformas estructurales han impactado en el sistema de pensiones, el cual debería garantizar estabilidad a los trabajadores en el momento que culminan su vida laboral, sin embargo, se han adoptado medidas que provocan el debilitamiento e incertidumbre en el sistema y, en el corto plazo, se puede presentar su colapso, siendo un reflejo del fracaso del mismo para garantizar un retiro digno a la clase trabajadora. En particular, el objetivo de esta colaboración es explicar las asimetrías en el sistema de pensiones en México, caracterizado por ser insuficiente y diferenciado, y que ha generado ganancias a los operadores de los recursos, pero sin impacto positivo en los ahorros de los trabajadores.</p><div><div><p> </p></div></div>