bluuebunny/crossref_metadata_2025_split huggingface dataset:
An intra‐cultural investigation in Brazil using Coalho cheese and preferred attribute elicitation
Authors: Soares Eveline K. B. , Silva Ramon , da Silva Wanessa P. , Kuriya Shigeno P. , Maçaira Paula M. , Cyrino Oliveira Fernando L. , da Silva Maria A. A. P. , Pimentel Tatiana C. , Freitas Mônica Q. , Cruz Adriano G. , Esmerino Erick A. 
Published: October 2019

AbstractThis study aimed to evaluate the application of preferred attribute elicitation (PAE) methodology for assessing the perceptions of consumers from different regions of Brazil (Northeast, Southeast or South, n = 20) about Coalho cheese samples. Northeast Brazilian consumers elicited a higher number of attributes (22 vs. 13–15) and had a higher frequency of consumption (3 vs. 1.3–1.6 times a week), while those from South region had higher reliability (RV = 0.95) with a low number of consumers (n = 16). Consumers from different Brazilian regions elicited common sensory descriptors considered important for Coalho cheese characterization, such as yellow color, presence of eyes, brightness, Coalho cheese aroma, salty taste, bitter taste, acid taste, and rubbery texture. Furthermore, there was no impact of the region on the acceptance of the products. In conclusion, Brazilian consumers from different regions characterized the Coalho cheeses in a similar manner, and PAE methodology could be used in intra‐cultural investigations, being recommended a minimum of 19 consumers.Practical ApplicationsConsumer based methodologies are gaining popularity in the sensory and consumer science. This is the first study that evaluated the application of PAE methodology regards an intra‐cultural consumer perception using as stimulus Coalho cheese, a typical Brazilian cheese. The findings are relevant to improve the processing of the Coalho cheese taking in account the consumer perception as well as to develop efficient marketing strategies of commercialization of this product.
Effects of over‐load iron on nutrient digestibility, haemato‐biochemistry, rumen fermentation and bacterial communities in sheep
Authors: Wang Yingjie , Jiang Mengyu , Zhang Zhongyuan , Sun Haixia 
Published: October 2019

AbstractThere is a risk of iron overload in grazing livestock. However, the effects on nutrient absorption and rumen function induced by excessive iron have not been well understood. Therefore, the purpose of present study was to investigate the impact of over‐load iron on growth performance, nutrient digestibility, blood biochemistry, rumen fermentation and bacterial communities in sheep. Twenty‐four German Mutton Merino cross‐bred sheep with weight (42.66 ± 2.34 kg BW) were randomly divided into 4 groups, each with 6 replicates and 1 sheep per replicate. The basal diet consisted of 60% Leymus chinensis hay and 40% concentrate. The sheep in 4 groups were fed the basal diets supplemented with 50 (Control), 500 (T1), 1,000 (T2) and 1,500 (T3) mg Fe/kg as ferrous sulphate monohydrate (FeSO4·H2O) respectively. And the actual contents of iron in the diet were determined to be 457.68 (control), 816.42 (T1), 1,256.78 (T2) and 1,725.63 (T3) mg/kg respectively. The experiment lasted 62 days including a 7‐day metabolism trial. During the whole experiment, the digestibility of dry matter, organic matter, neutral detergent fibre and acid detergent fibre showed a quadratic increase with increasing over‐load iron levels (p < .05), and maximum responses were found with 500 mg/kg supplementation. However, the response of total VFA concentration showed a quadratic decrease, as did the concentrations of propionate, butyrate and valerate (p < .05). Serum total iron‐binding capacity on day 30 showed a quadratic decrease with the increase in high‐dose iron, while the serum iron content increased linearly at day 60 (p < .05). Excessive iron resulted in the change in bacterial communities. An increase in over‐load iron linearly decreased the abundance of bacteria in the phylum Bacteroidetes (p < .05), but linearly increased the Firmicutes (p = .037) and Proteobacteria (p = .018). In addition, there was a quadratic effect (p = .003) on the Fibrobacteres, which was higher in the 500 and 1,000 mg/kg Fe‐supplemented groups. At the genus level, there were quadratic effects on the abundances of Selenomonas_1 (p = .023) and Ruminococcaceae_UCG‐014 (p = .016). Furthermore, feeding of iron linearly increased the relative abundances of Succiniclasticum and Succinivibrionaceae_UCG‐002 (p < .05). These results indicate that increasing ferrous sulphate monohydrate in diets had no negative impact on the growth performance, but it changed nutrient digestibility, blood iron parameters, rumen fermentation and bacterial communities in sheep.
Loss to follow‐up: A significant barrier in the treatment cascade with direct‐acting therapies
Authors: Darvishian Maryam , Wong Stanley , Binka Mawuena , Yu Amanda , Ramji Alnoor , Yoshida Eric M. , Wong Jason , Rossi Carmine , Butt Zahid A. , Bartlett Sofia , Pearce Margo E. , Samji Hasina , Cook Darrel , Alvarez Maria , Chong Mei , Tyndall Mark , Krajden Mel , Janjua Naveed Z. 
Published: October 2019

AbstractEffectiveness of direct‐acting antiviral (DAA) therapies could be influenced by patient characteristics such as comorbid conditions, which could lead to premature treatment discontinuation and/or irregular medical follow‐ups. Here, we evaluate loss to follow‐up and treatment effectiveness of sofosbuvir/ledipasvir ± ribavirin (SOF/LDV ± RBV), ombitasvir/paritaprevir/ritonavir + dasabuvir ± ribavirin (OBV/PTV/r + DSV ± RBV) for hepatitis C virus (HCV) genotype 1 (GT1) and sofosbuvir + ribavirin (SOF + RBV) for genotype 3 (GT3) in British Columbia Canada: The British Columbia Hepatitis Testers Cohort includes data on individuals tested for HCV since 1992, integrated with medical visit, hospitalization and prescription drug data. HCV‐positive individuals who initiated DAA regimens, irrespective of treatment completion, for GT1 and GT3 until 31 December, 2017 were included. Factors associated with sustained virological response (SVR) and loss to follow‐up were assessed by using multivariable logistic regression models. In total 4477 individuals initiated DAAs. The most common prescribed DAA was SOF/LDV ± RBV with SVR of 95%. The highest SVR of 99.5% was observed among OBV/PTV/r + DSV‐treated patients. Overall, 453 (10.1%) individuals were lost to follow‐up. Higher loss to follow‐up was observed among GT1 patients treated with OBV (17.8%) and GT3 patients (15.7%). The loss to follow‐up rate was significantly higher among individuals aged <60 years, those with a history of injection drug use (IDU), on opioid substitution therapy and with cirrhosis. Our findings indicate that loss to follow‐up exceeds viral failure in HCV DAA therapy and its rate varies significantly by genotype and treatment regimen. Depending on the aetiology of lost to follow‐up, personalized case management for those with medical complications and supporting services among IDU are needed to achieve the full benefits of effective treatments.
Enteroinsular axis response to carbohydrates and fasting in healthy newborn foals
Authors: Rings Lindsey M. , Swink Jacob M. , Dunbar Laura K. , Burns Teresa A. , Toribio Ramiro E. 
Published: October 2019

AbstractBackgroundThe enteroinsular axis (EIA) comprises intestinal factors (incretins) that stimulate insulin release after PO ingestion of nutrients. Glucose‐dependent insulinotropic polypeptide (GIP) and glucagon‐like peptide‐1 (GLP‐1) are the main incretins. The EIA has not been investigated in healthy neonatal foals but should be important because energy demands are high in healthy foals and dysregulation is frequent in sick foals.Objectives and HypothesisTo evaluate the EIA response to carbohydrates or fasting in newborn foals. We hypothesized that incretin secretion would be higher after PO versus IV carbohydrate administration or fasting.AnimalsThirty‐six healthy Standardbred foals ≤4 days of age.MethodsProspective study. Blood was collected before and after a PO glucose test (OGT; 300, 500, 1000 mg/kg), an IV glucose test (IVGT; 300, 500, 1000 mg/kg), a PO lactose test (OLT; 1000 mg/kg), and fasting. Foals were muzzled for 240 minutes. Blood was collected over 210 minutes glucose, insulin, GIP, and GLP‐1 concentrations were measured.ResultsOnly PO lactose caused a significant increase in blood glucose concentration (P < .05). All IV glucose doses induced hyperglycemia and hyperinsulinemia. Concentrations of GIP and GLP‐1 decreased until foals nursed (P < .05), at which time rapid increases in glucose, insulin, GIP, and GLP‐1 concentrations occurred (P < .05).Conclusions and Clinical ImportanceHealthy newborn foals have a functional EIA that is more responsive to milk and lactose than glucose. Non‐carbohydrate factors in mare's milk may be important for EIA activity. Constant exposure of intestinal cells to nutrients to maintain EIA activity could be relevant to management of sick foals. Foals can be fasted for 4 hours without experiencing hypoglycemia.
Long‐term monitoring of a highly invaded annual grassland community through drought, before and after an unintentional fire
Authors: Thomson Diane M. , Bonapart Adin D. , King Rachel A. , Schultz Emily L. , Startin Charlotte R. 
Published: October 2019

AbstractQuestions(a) How did seedling numbers and species composition change in the first year after a wildfire during drought, relative to pre‐fire variation? (b) Has the community returned to pre‐fire composition after five years? (c) Has the degree of dominance by exotic annual grasses changed? (d) Is there any evidence that drought conditions affected community cover, before or after fire?LocationExotic‐dominated annual grassland in southern California, USA.MethodsWe monitored community cover and native annual forb densities for four years before and four (cover) to five (densities) years after an unintentional fire (fall 2013) coinciding with the spring 2012–2019 California drought. We also measured seedling emergence both before and during the first year post‐fire. We assessed post‐fire changes in cover and density relative to pre‐fire variation, and tested correlations between community cover and annual rainfall measures.ResultsSeedling emergence declined strongly after fire for exotic grasses, but remained stable for exotic forbs. Seedling densities of the most common native forbs declined, but several previously‐rare natives increased. Community cover initially shifted towards the exotic forbs Erodium spp., then returned to higher exotic grass densities. Yet the previously dominant Bromus diandrus declined steeply, even as other exotic grasses and some native forbs increased. Up to five years after fire, relative cover and abundance of the most common exotic and native species still differed from pre‐fire composition. Common species were uncorrelated with annual precipitation, but several may have responded to shorter growing seasons.ConclusionsImmediate post‐fire conditions favoured exotic and native forbs over grasses, as predicted. Yet in contrast to many previous studies, the community did not return quickly to pre‐fire composition but showed persistent changes that favoured neither natives nor exotics. Our results suggest post‐fire recovery in this habitat may be contingent on abiotic conditions, with drought one potential explanation for changes.
Large‐scale genomic sequence data resolve the deepest divergences in the legume phylogeny and support a near‐simultaneous evolutionary origin of all six subfamilies
Authors: Koenen Erik J. M. , Ojeda Dario I. , Steeves Royce , Migliore Jérémy , Bakker Freek T. , Wieringa Jan J. , Kidner Catherine , Hardy Olivier J. , Pennington R. Toby , Bruneau Anne , Hughes Colin E. 
Published: October 2019

Summary

Phylogenomics is increasingly used to infer deep‐branching relationships while revealing the complexity of evolutionary processes such as incomplete lineage sorting, hybridization/introgression and polyploidization. We investigate the deep‐branching relationships among subfamilies of the Leguminosae (or Fabaceae), the third largest angiosperm family. Despite their ecological and economic importance, a robust phylogenetic framework for legumes based on genome‐scale sequence data is lacking.

We generated alignments of 72 chloroplast genes and 7621 homologous nuclear‐encoded proteins, for 157 and 76 taxa, respectively. We analysed these with maximum likelihood, Bayesian inference, and a multispecies coalescent summary method, and evaluated support for alternative topologies across gene trees.

We resolve the deepest divergences in the legume phylogeny despite lack of phylogenetic signal across all chloroplast genes and the majority of nuclear genes. Strongly supported conflict in the remainder of nuclear genes is suggestive of incomplete lineage sorting.

All six subfamilies originated nearly simultaneously, suggesting that the prevailing view of some subfamilies as ‘basal’ or ‘early‐diverging’ with respect to others should be abandoned, which has important implications for understanding the evolution of legume diversity and traits. Our study highlights the limits of phylogenetic resolution in relation to rapid successive speciation.
Microbiome‐immune‐metabolic axis in the epidemic of childhood obesity: Evidence and opportunities
Authors: Kincaid Halle J. , Nagpal Ravinder , Yadav Hariom 
Published: October 2019

SummaryObesity epidemic responsible for increase in diabetes, heart diseases, infections and cancer shows no signs of abating. Obesity in children is also on rise, indicating the urgent need of strategies for prevention and intervention that must begin in early life. While originally posited that obesity results from the simple concept of consuming more calories, or genetics, emerging research suggests that the bacteria living in our gut (gut microbiome) and its interactions with immune cells and metabolic organs including adipose tissues (microbiome‐immune‐metabolic axis) play significant role in obesity development in childhood. Specifically, abnormal changes (dysbiosis) in the gut microbiome, stimulation of inflammatory cytokines, and shifts in the metabolic functions of brown adipose tissue and the browning of white adipose tissue are associated with increased obesity. Many factors from as early as gestation appear to contribute in obesity, such as maternal health, diet, antibiotic use by mother and/or child, and birth and feeding methods. Herein, using evidence from animal and human studies, we discuss how these factors impact microbiome‐immune‐metabolic axis and cause obesity epidemic in children, and describe the gaps in knowledge that are warranted for future research.
Prediction of Collapse Using Patient‐Specific Finite Element Analysis of Osteonecrosis of the Femoral Head
Authors: Li Tai‐xian , Huang Ze‐qing , Li Yan , Xue Zhi‐peng , Sun Ji‐gao , Gao Huan‐huan , He Hai‐jun , Chen Wei‐heng 
Published: October 2019

ObjectiveTo develop a prediction method for femoral head collapse by using patient‐specific finite element analysis of osteonecrosis of the femoral head (ONFH).MethodsThe retrospective study recruited 40 patients with ARCO stage‐II ONFH (40 pre‐collapse hips). Patients were divided into two groups according to the 1‐year follow‐up outcomes: patient group without femoral head collapse (noncollapse group, n = 20) and patient group with collapse (collapse group, n = 20). CT scans of the hip were performed for all patients once they joined the study. Patient‐specific finite element models were generated based on these original CT images following the same procedures: segmenting the necrotic lesion and viable proximal femur, meshing the computational models, assigning different material properties according to the Hounsfield unit distribution, simulating the stress loading of the slow walking gait, and measuring the distribution of the von Mises stress. Receiver operating characteristic (ROC) curve analysis was used to evaluate the predictive performance of the maximum level of the von Mises stress. The optimal cut‐off value was selected based on the Youden index and the corresponding predictive accuracy was reported as well.ResultsThe mean level of the maximum von Mises stress in the collapse group was 2.955 ± 0.539 MPa, whereas the mean stress level in the noncollapse group was 1.923 ± 0.793 MPa (P < 0.01). ROC analysis of the maximum von Mises stress found that the area under the ROC curve was 0.842 (95% CI: 0.717–0.968, P < 0.01). The maximum Youden index was 0.60, which corresponded to two optimal cut‐off values: 2.7801 MPa (sensitivity: 0.70; specificity: 0.90; predictive accuracy: 80.00%; LR+: 7), and 2.7027 MPa (sensitivity: 0.75; specificity: 0.85; predictive accuracy: 77.50%; LR+: 5).ConclusionFinite element analysis is a potential method for femoral head collapse prediction among pre‐collapse ONFH patients. The maximum level of the von Mises stress on the weight‐bearing surface of the femoral head could be a good biomechanical marker to classify the collapse risk. The collapse prediction method based on patient‐specific finite element analysis is, thus, suitable to apply to clinical practice, but further testing on a larger dataset is desirable.
Hip Replacement as Alternative to Intramedullary Nail in Elderly Patients with Unstable Intertrochanteric Fracture: A Systematic Review and Meta‐Analysis
Authors: Ju Jia‐bao , Zhang Pei‐xun , Jiang Bao‐guo 
Published: October 2019

ObjectiveTo evaluate the efficacy and safety of hip replacement and intramedullary nails for treating unstable intertrochanteric fractures in elderly patients.MethodsRandomized clinical trials (RCTs) to compare hip replacement with intramedullary nail in the management of elderly patients with unstable intertrochanteric femur fracture were retrieved from Cochrane Library (up to January 2018), CNKI (China National Knowledge Infrastructure), Wanfang Data, PubMed, and Embase. The methodological quality of the included trials was assessed using the Cochrane risk of bias assessment tool, and relevant data was extracted. Statistical analysis was performed by Revman 5.3. Where possible, we performed the limited pooling of data.ResultsFourteen trials including a total of 1067 participants aged 65 and above were included for qualitative synthesis and meta‐analysis. The methodological quality of the included study was poor. The meta‐analysis indicated that the hip replacement group benefited more than the intramedullary nail group in terms of the bearing load time (WMD ‐14.61, 95% CI −21.51 to −7.7, P < 0.0001), mechanical complications (OR 0.34, 95% CI 0.21 to 0.57, P < 0.0001), and post‐operative complications (OR 0.46, 95% CI 0.22 to 0.93, P = 0.03). While the intramedullary nail was superior to arthroplasty regarding the intraoperative blood loss (WMD 58.36, 95% CI 30.77 to 85.94, P < 0.0001). However, there were no statistical significances in the length of surgery (WMD 5.27, 95% CI 4.23 to 14.77, P = 0.28), units of blood transfusion (WMD 0.34, 95% CI ‐0.16 to 0.85, P = 0.18), length of hospital stay (WMD ‐1.00, 95% CI ‐2.93 to 0.93, P = 0.31), Harris hip score (WMD 0.31, 95% CI ‐0.39 to 1.01, P = 0.38), and mortality (OR 1.24, 95% CI 0.12 to 13.10, P = 0.86).ConclusionsThis systematic review and meta‐analysis provided evidence for the efficacy and safety of hip replacement and intramedullary nail in treating unstable intertrochanteric fractures. However, the results should be interpreted cautiously because of methodological limitations and publication bias.
Scarf Osteotomy for Correction of Hallux Valgus Deformity in Adolescents
Authors: Wang Xin‐wen , Wen Qian , Li Yi , Liu Cheng , Zhao Kai , Zhao Hong‐mou , Liang Xiao‐jun 
Published: October 2019

ObjectiveTo report the radiological and clinical outcomes of the modified scarf osteotomy for the treatment of hallux valgus deformity in adolescents.MethodsThis retrospective study analyzed 21 patients (31 feet) who underwent a modified scarf osteotomy for correcting juvenile hallux valgus deformity between March 2015 and January 2017. There were 3 male (3 feet) and 18 female (28 feet) patients. The average age at the time of surgery was 28.6 years (range, 20–35). Patients were postoperatively followed up in the outpatient department for 12–18 months. Clinical and radiological assessments were performed preoperatively and postoperatively at 1 year. Moreover, postoperative complications were recorded. Statistical analyses for differences between preoperative and postoperative values were performed.ResultsAll the 21 patients were postoperatively followed up for 12–18 months, with an average of 13.2 ± 2.5 months. Clinical assessment showed that the American Orthopaedic Foot and Ankle Society score was increased from preoperative 58.0 ± 5.8 to postoperative 94.2 ± 6.6 points, respectively, and the visual analog scale score was remarkably decreased from preoperative 6.0 ± 2.0 to postoperative 1.5 ± 2.0 points at 1 year follow‐up. Further radiological assessment showed that the hallux valgus angle was 37.5° ± 9.2°, 14.1° ± 6.5°, and 14.5° ± 6.5° before surgery, half a year after surgery, and 1 year after surgery, respectively; the intermetatarsal angle was 14.1° ± 4.4°, 4.8° ± 3.2°, and 5.5° ± 4.9°, respectively; and the distal metatarsal articular angle was 31.0° ± 3.5°, 7.2° ± 2.3°, and 7.5° ± 2.1°, respectively. They were significantly improved at half a year after surgery and 1 year after surgery compared to those before surgery. Complications occurred in two patients (9.5%) who had numbness on the skin of the edge of the medial incision, and the symptoms were relieved after 10 months. There was no clinical recurrence in all patients. One of the 31 feet had hallux varus, which was corrected in a second operation. Notably, a postoperative radiograph of a typical case whose both feet had hallux valgus deformity and underwent modified scarf osteotomy and additional Akin osteotomy showed adequate correction of the hallux valgus angle (HVA, 11°), intermetatarsal angle (IMA, 6°), and distal metatarsal articular angle (DMAA, 8°) on left foot compared to preoperative HVA (28°), IMA (13°), and DMAA (35°).ConclusionThe modified scarf osteotomy can effectively correct the adolescent hallux valgus deformity, which is worth popularizing.
Moving to safety and staying in school: The effects of violence on enrollment decisions in Mexico
Authors: Márquez‐Padilla Fernanda , Pérez‐Arce Francisco , Rodríguez‐Castelán Carlos 
Published: October 2019

AbstractThere is a vast literature documenting negative impacts of crime on human capital accumulation, which has focused on large‐scale armed conflicts affecting both individuals and infrastructure. However, there is much less evidence in contexts where violence is not accompanied by the destruction of infrastructure. To fill this gap, this paper studies the effects of Mexico's War on Drugs (WoD) on individuals’ schooling decisions. Our results show only small effects of violence on total enrollment of children and young adults, in contrast to recent studies which find significant negative effects for some specific age‐by‐gender groups. Our analysis, which aims to assess the overall impacts of the WoD on enrollment decisions and takes advantage of various rounds of administrative data, the population census, and labor force surveys, shows that many families responded to the increase in violence by migrating to less affected municipalities. However, we find that their education decisions have been quite robust as greater violence did not affect their probability of being enrolled in school. In the long term, however, it is still possible that the increase in violence affects human capital accumulation through indirect effects arising in skill acquisition in early childhood and other stages.