bluuebunny/crossref_metadata_2025_split huggingface dataset:
Insights Into the Mechanism of MCT8 Oligomerization
Authors: Groeneweg Stefan , van den Berge Amanda , Lima de Souza Elaine C , Meima Marcel E , Peeters Robin P , Visser W Edward 
Published: June 2020

AbstractMutations in the thyroid hormone transporter monocarboxylate transporter 8 (MCT8) result in MCT8 deficiency, characterized by severe intellectual and motor disability. The MCT8 protein is predicted to have 12 transmembrane domains (TMDs) and is expressed as monomers, homodimers, and homo-oligomers. This study aimed to delineate the mechanism of MCT8 oligomerization. Coimmunoprecipitation studies demonstrated that lithium dodecyl sulfate effectively disrupts MCT8 protein complexes, indicating the involvement of non-covalent interactions. Successive C-terminal truncations of the MCT8 protein altered the oligomerization pattern only if introduced in the N-terminal half of the protein (TMD1-6). The truncation at extracellular loop 1 (E206X) still allowed homodimerization, but completely abrogated homo-oligomerization, whereas both were preserved by the C231X mutant (at TMD2), suggesting that the minimally required oligomerization sites are located proximal of Cys231. However, mutant constructs lacking the intracellular N-terminus or TMD1 and 2 were still capable to form homo-oligomers. Therefore, other domains distal of Cys231 are also likely to be involved in the formation of extensive multidomain interactions. This hypothesis was supported by structural modeling. Despite multiple approaches, MCT8 oligomerization could not be fully abrogated unless a substantial part of the protein was removed, precluding detailed studies into its functional role. Together, our findings suggest that MCT8 oligomerization involves extensive noncovalent interactions between the N-terminal halves of MCT8 proteins. Most mutations identified in patients with MCT8 deficiency have only minor effects on MCT8 oligomerization and, thus, impaired oligomerization does not appear to be an important pathogenic mechanism.
Obsessive Compulsive Disorder: Frequency and Gender Estimates
Authors: Jabeen Shaista , Kausar Rukhsana 
Published: June 2020

Objective: Frequency data on mental disorders is a crucial requirement for primary, secondary and tertiary prevention. However, such local data is sparse. The study aimed to estimate frequency and gender differences of Obsessive Compulsive Disorder (OCD) in clinical population.
Method: Retrospective data of adult patients (18 years and above) reported in Out Patient Departments (OPD) of four teaching hospitals and a private clinic was collected. Successive three years record was consulted using OPD registers. Gender difference in frequency of OCD was estimated.
Results: Altogether 90119 patients were registered in five psychiatric settings in major cities of Punjab during three year period. As information was missing for over one third of the registered patients, hence analysis was carried out on valid cases only i.e. 59220 (65.8% of the total number of patients registered). It was revealed that the estimated frequency of OCD is 4.1%. There was no significant gender difference revealed in frequency of OCD (P>0.05).
Conclusion: Frequency of OCD implicate the need for early detection and intervention of the disorder. Further, it elucidates the importance of community based research on other mental disorders. The need for accurate record keeping which is a core element for any research related with medical or psychological issue is also highlighted.
doi: https://doi.org/10.12669/pjms.36.5.1870
How to cite this:Jabeen S, Kausar R. Obsessive Compulsive Disorder: Frequency and Gender Estimates. Pak J Med Sci. 2020;36(5):1048-1052. doi: https://doi.org/10.12669/pjms.36.5.1870
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Reverse logistics performance measures: a survey of Brazilian companies
Authors: Chaves Gisele de Lorena Diniz , Giuriatto Naiara Tomazelli , Ferreira Karine Araujo 
Published: June 2020

Goal: The purpose of this research was to determine the most frequently used performance measures for the evaluation of reverse logistics in Brazilian companies. Furthermore, we sought to verify whether a correlation exists between certain performance measure dimensions (cost, asset management, customer service, and productivity), company size (micro, small, medium, and large), and their sectors of the economy.
Design/Methodology/Approach: A survey of 125 Brazilian companies from different industrial sectors was conducted. Hypothesis testing allows carrying out inferences about population parameters, and the results indicate the acceptance or rejection of the hypothesis formulated.
Results: This study presents a list of measures for the assessment of reverse logistics in different industrial segments, evidencing the major performance measures used. According to statistical analysis, large-sized companies are those that most frequently adopt performance measures to assess reverse logistics in Brazil. However, no correlation was verified between the performance measure dimensions evaluated and the sectors of the economy.
Limitations of investigation: This study could not conclude about the maturity level of performance measurement in the investigated companies. The results presented are the first step towards reverse logistics performance measurement and more complementary studies are necessary.
Practical implications: This paper presents a list of performance measures for reverse logistics assessment. This information could support decision making as it provides a possibility of analyzing the choice of the best measures.
Originality/value: The main contribution of this paper is related to the scarcity of reverse logistics metrics in the literature. As an exploratory study, it could be used to improve the reverse logistics performance measurement studies and practice.
Seasonal incidence and bionomics of rose aphid, Macrosiphum rosae (Linnaeus, 1758), (Hemiptera: Aphididae) in Kashmir, India
Authors: YOUSUF Insha , BUHROO Abdul A. 
Published: June 2020

Rose is the principal flower of the world floriculture industry that is being exclusively used as cut flower, potted plant and garden plant. It plays significant part in numerous industries viz. food, perfumery and cosmetic industries. About 96 % of women’s perfumes contain true Bulgarian rose oil. Roses are well acclimatized in Jammu & Kashmir because of its suitable agro climatic conditions which can permit its large scale production and rose products produced in the state are at par with the international standards. But the aesthetic and commercial value of roses is greatly lowered by numerous insect pests resulting in low yield. However, its major pest include aphid species most notoriously Macrosiphum rosae that pose many challenges and threats to rose plant cultivation. Aphid colonies on roses result in reduction of medical value of the plant and cause economic losses to growers particularly during spring and summer season. In order to reduce the economic losses inflicted by rose aphid, it is necessary to study different biological parameters of this pest species so that an effective management plan can be formulated.
Effect of foliar or soil application of selenium on some morphological and physiological traits of garden pansy (Viola x wittrockiana Gams) grown under salinity stress
Authors: JAVADI Farima , KALATEJARI Sepideh , DIYANAT Marjan 
Published: June 2020

Salinity stress is one of the most important plant stresses in Iran. In this regard, a factorial experiment was conducted to investigate the effects of salinity stress on the garden pansy. The investigated factors were containing sodium selenate (0, 2, 4 and 8 mg l-1), its method of application (foliar and soil applications) and salinity stress (0, 3 and 6 dS m-1). The obtained results indicated that salinity leads to the significant reduction in morphological traits, chlorophyll a and b contents. Under the salinity of 6 dS m-1, when sodium selenate was used in the soil, the fresh and dry mass of flower increased by 11.34 and 10.39 %, respectively, compared to the control. However, the use of sodium selenate by foliar application led to the increasing fresh and dry mass of garden pansy’s flower by 25.10 and 25.41 %, respectively. Also, the content of chlorophyll a increased by 12.93 % under the salinity of 6 dS m-1 with applying 8 mg l-1sodium selenate compared to the case of non-application. The superoxide dismutase activity decreased by 26.13 % compared to the non-sodium selenate usage treatment. In conclusion the foliar application of sodium selenate at the concentraion of 8 mg l-1 resulted in the garden pansy’s growth improvement.
Influence of edaphoclimatic conditions on stem production and stem morphological characteristics of 10 European hemp (Cannabis sativa L.) varieties
Authors: FLAJŠMAN Marko , KOCJAN AČKO Darja 
Published: June 2020

Six dioecious (Antal, KC Dóra, Kompolti hibrid TC, Monoica, Tiborszallasi and Tisza) and four monoecious (Fedora 17, Futura 75, Santhica 27 and USO 31) European hemp varieties were sown at a density of 300 viable seeds per m2 and a row spacing of 12.5 cm in a three-year field trial (2017-2019) to evaluate the yield of the stems (fresh and dry) and some other biometric characteristics of the stems. No pesticides were used during plant growth to suppress weeds, diseases and pests. The highest yield of fresh and dry stems was achieved by the variety Antal with 12.3 t ha-1 and 5.3 t ha-1, respectively, followed by the varieties Futura 75 and Tiborszallasi. The lowest yields of fresh and dry stems were recorded for the monecious variety USO 31 (6.0 and 2.6 t ha-1). In general, dioecious varieties had higher and thicker stems than monoecious varieties. The year of production had a highly significant impact on all variables, in particular on the proportion of weed biomass, which was the highest in 2019 (77.2 %), when weather conditions were most unfavourable for hemp cultivation. The correlation analysis between fresh/dry stem yields and weed biomass was highly negative (-0.85 and -0.83) and strongly statistically significant (p < 0.001), indicating the issue related to weed management.
Systematic Screen forDrosophilaTranscriptional Regulators Phosphorylated in Response to Insulin/mTOR Pathway
Authors: Liu Ying , Mattila Jaakko , Hietakangas Ville 
Published: June 2020

AbstractInsulin/insulin-like growth factor signaling (IIS) is a conserved mechanism to regulate animal physiology in response to nutrition. IIS activity controls gene expression, but only a subset of transcriptional regulators (TRs) targeted by the IIS pathway is currently known. Here we report the results of an unbiased screen for Drosophila TRs phosphorylated in an IIS-dependent manner. To conduct the screen, we built a library of 857 V5/Strep-tagged TRs under the control of Copper-inducible metallothionein promoter (pMt). The insulin-induced phosphorylation changes were detected by using Phos-tag SDS-PAGE and Western blotting. Eight proteins were found to display increased phosphorylation after acute insulin treatment. In each case, the insulin-induced phosphorylation was abrogated by mTORC1 inhibitor rapamycin. The hits included two components of the NURF complex (NURF38 and NURF55), bHLHZip transcription factor Max, as well as the Drosophila ortholog of human proliferation-associated 2G4 (dPA2G4). Subsequent experiments revealed that the expression of the dPA2G4 gene was promoted by the mTOR pathway, likely through transcription factor Myc. Furthermore, NURF38 was found to be necessary for growth in larvae, consistent with the role of IIS/mTOR pathway in growth control.
Clinical Impact of Plasma and Tissue Next-Generation Sequencing in Advanced Non-Small Cell Lung Cancer: A Real-World Experience
Authors: Bonanno Laura , Pavan Alberto , Ferro Alessandra , Calvetti Lorenzo , Frega Stefano , Pasello Giulia , Aprile Giuseppe , Guarneri Valentina , Conte PierFranco ,   on behalf of Rete Oncologica Veneta (ROV)
Published: June 2020

Abstract

Background
Targeted agents have improved the outcome of a subset of non-small cell lung cancer (NSCLC). Molecular profiling by next-generation sequencing (NGS) allows screening for multiple genetic alterations both in tissue and in plasma, but limited data are available concerning its feasibility and impact in real-world clinical practice.

Methods
Patients with advanced NSCLC consecutively referring to our Institution for potential eligibility to VISION trial (NCT02864992) were prospectively enrolled. They were already screened with standard method, and EGFR/ALK/ROS-1 positive cases were excluded. NGS was performed in plasma and tissue using the Guardant360 test covering 73 genes and the Oncomine Focus Assay covering 59 genes, respectively.

Results
The study included 235 patients. NGS was performed in plasma in 209 (88.9%) cases; 78 of these (37.3%) were evaluated also in tissue; tissue only was analyzed in 26 cases (11.1%). Half of the tissue samples were deemed not evaluable. Druggable alterations were detected in 13 (25%) out of 52 evaluable samples and 31 of 209 (14.8%) of plasma samples. Improved outcome was observed for patients with druggable alterations if treated with matched targeted agents: they had a longer median overall survival (not reached) compared with the ones who did not start any targeted therapy (9.1 months; 95% confidence interval, 4.6–13.6; p = .046). The results of NGS testing potentially also affected the outcome of patients treated with immunotherapy.

Conclusion
Systematic real-life NGS testing showed the limit of tissue analysis in NSCLC and highlighted the potentiality of genetic characterization in plasma in increasing the number of patients who may benefit from NGS screening, both influencing the clinical decision-making process and affecting treatment outcome.

Implications for Practice
Genetic characterization of cancer has become more important with time, having had positive implications for treatment specificity and efficacy. Such analyses changed the natural history of advanced non-small cell lung cancer (aNSCLC) with the introduction of drugs targeted to specific gene alterations (e.g., EGFR mutations, ALK and ROS-1 rearrangements). In the field of cancer molecular characterization, the applicability of the analysis of a wide panel of genes using a high-throughput sequencing approach, such as next-generation sequencing (NGS), is still a matter of research. This study used NGS in a real-world setting to systematically and prospectively profile patients with aNSCLC. The aim was to evaluate its feasibility and reliability, as well as consequent access to targeted agents and impact on clinical outcome whenever a druggable alteration was detected either in tumor tissue samples or through liquid biopsy.
North American Yoga Practitioners’ Lived Experiences of Mind-Body Connection: A Phenomenological Study
Authors: Weinstein Rachel 
Published: June 2020

Abstract
The practice of yoga was born in India thousands of years ago and brought to North America gradually beginning in the 20th century. The traditional practice of yoga is spiritual in nature with an intention of purifying the mind and body, leading to an alleviation of suffering through connection with the Divine (i.e., liberation). Yoga has gained widespread popularity in North America, but whether North American yoga practice includes an intention on the purification of the mind-body, in contemporary practice often described as a mind-body connection, has yet to be explored. This research study investigated North American yoga practitioners’ experiences of mind-body connection in their practices. Six yoga practitioners residing in North America were interviewed for this study. Interviews were audiorecorded and transcribed. Phenomenological analysis was conducted to produce the essence data, and thematic analysis was conducted to produce the contextual data. Phenomenological themes regarding the co-researchers’ experiences of mind-body connection in their yoga practices were identified and grouped into four textural essences: (1) the experience of breath, (2) local or internal experiences, (3) an increased sense of awareness and mindfulness, and (4) transcendental and spiritual qualities. Four structural conditions that allow practitioners to experience mind-body connection during yoga practice were identified: (1) breath, (2) physical asana, (3) practitioner intentionality, and (4) environmental conditions. Findings of the current study suggest a capacity for North American yoga practitioners to experience mind-body connection that is essential to traditional yoga practice.
TERSİNE LOJİSTİK TEORİ VE UYGULAMALARININ SON 10 YILI: BİBLİYOMETRİK BİR ANALİZ
Authors: ERTURGUT Ramazan , GÜRLER Hasan Emin 
Published: June 2020

Dünya ticaretinin değişen
dinamikleri, üretim teknolojilerindeki ve tüketim alışkanlıklarındaki kayda
değer farklılaşmalar yanında, Sivil Toplum örgütlerinin gittikçe güçlenmesi ve
hemen her alanda sürdürülebilirlik düşünce ve uygulamalarının yaygınlık
kazanması, ürünlerin geri kazanımı ve çevreye zarar vermeksizin bertaraf
edilmesi konusunda toplumların bilinçlilik düzeyini artırmıştır. Toplumların, uluslararası
örgütlerin ve STK’ların konuya artan bu ilgi düzeyi, lojistik yönetiminde ürün
geri kazanımı, yeniden üretim ve ürünlerin bertarafı veya tamir için geri
çağırma olarak bilinen tersine lojistik görüş ve uygulamalarına da daha fazla
önem atfedilmesine yol açmıştır. Bu çalışmanın odak ilgisi, son on yılda
tersine lojistik alanında ortaya konulan bilimsel çıktıların incelenerek
araştırma ve uygulamaların seyrini nitel bir çözümleme ile ortaya koymaktır. Bu amaç doğrultusunda Web of Science
veritabanında tersine lojistik, yeniden üretim, yeniden kullanım, geri kazanım,
geri dönüşüm, yok etme, tersine akış, yenileme gibi konuya ilişkin anahtar
kelimeler kullanılarak tarama yapılmış ve toplamda 701 yayına ulaşılmıştır. Araştırma
yöntemi olarak Bibliyometrik Analiz ve İçerik Analizi yöntemleri birlikte
kullanılmıştır. Araştırma sonuçları; 2015 yılından itibaren niceliksel bir
yoğunlaşma olduğunu, araştırma makalesi ve ampirik analiz eksenli yayınların
ağırlık kazandığını ve üniversiteler bazında
Tersine Lojistik temalı en fazla
yayın yapan üniversitenin ABD’de bulunan Auburn Üniversitesi olduğunu ortaya
koymuştur. Ayrıca, özetlerde en fazla değinilen kavramın tedairk zinciri
olduğu, en fazla çalışılan konunun kapalı döngü tedarik zinciri yönetimi olduğu
ve en fazla kullanılan yöntemin analitik hiyerarşi süreci yöntemi olduğu tespit
edilmiştir.
CONTRASTIVE APPROACH TO NUMERIC USAGE IN JAPAN AND IN THE ABAJADUN METHOD
Authors: Sunarni Nani , Kurnia Firmansyah Eka , Abdul Malik Zulfi , Rohmayani Yani 
Published: June 2020

Purpose: The article presents both numeric implementation in Japan and the Arabic gematrical calculation (hisababajadun) in Indonesia. It is common for the people in Japan and Indonesia to believe in the power of numerals and to assume whether or not certain digits induce bad luck.
Methodology: The research applies a qualitative descriptive method through a contrastive approach.
Main Findings: Data analysis is based on both Koizumi's approach (1995) to the meaning of numbers in the Japanese language and Al Bani's view on hisababajadun. Our study shows that the numeric calculation in Japan uses luckiness as its ground while the hisababajadun builds its mechanism on birth date calculation according to Quranic Neuro-Hypnosis.
Implications/ Applications: The research may enrich existing theoretical references on the belief toward numbers in both Japan and Indonesia. While in practice, it may as well be a reference for the application of numeric calculation methods in both cultures.
Novelty/Originality of this study: This study is unique and novel as it enhances the understanding of the importance of numerals in two distinct cultures i.e., Japan and Indonesia. Moreover, this study sheds light on some crucial aspects of numeral calculation including how these numerals are used, what beliefs are attached to them and how and where these numerals are used for solving life problems.
Desvelando as pesquisas em educação ambiental em contexto não escolar
Authors: Oliveira Araújo Maria Inêz , Nascibem Fábio Gabriel , Vilar de Almeida Renata 
Published: June 2020

Diante das alterações ambientais, em consequência das práticas antrópicas, é necessário desenvolver novas mentalidades que busquem caminhos para reduzir as pressões humanas na degradação ambiental. O artigo tem como objetivo apresentar caminhos trilhados em trabalhos de pesquisa em Educação Ambiental não formal. Para tanto, foi realizado um levantamento dos principais conceitos abordados e métodos utilizados nos artigos apresentados na comunicação oral do Grupo de Discussão de Pesquisa (GDP) em contextos não escolares, no X Encontro de Pesquisa em Educação Ambiental (EPEA), em 2019. Dos artigos avaliados, foram reconhecidas três categorias de conceitos: pertencimento, relação sociedade-natureza e o próprio conceito de educação ambiental; e duas categorias de método: oito artigos trabalharam a pesquisa com atividades formativas, e dez buscaram compreender o contexto e as opiniões dos participantes. Tais caminhos sinalizam uma postura de respeito à pluralidade de saberes e de manifestações, compatíveis com a pluralidade do mundo, na busca de concretizar uma educação ambiental dialógica, acessível, democrática, diversa, cidadã, política e crítica.
Croissance musculaire et fonction cardio-respiratoire chez le poulet de chair
Authors: SANCHEZ A. , PLOUZEAU M. , RAULT P. , PICARD M. 
Published: June 2020

Cet article fait le point sur l’influence supposée de la sélection du poulet de chair privilégiant croissance rapide, rendement musculaire élevé et indice de consommation minimum, sur la fréquence des incidents d’élevage d’origines cardio-vasculaire et respiratoire, aggravés par les coups de chaleur. Les poulets à croissance rapide présentent un rapport du volume pulmonaire sur le poids corporel inférieur, une capacité à fixer l’O2 dans le sang plus faible, une viscosité sanguine plus élevée et des arythmies cardiaques plus fréquentes que les poulets à croissance lente. Ces facteurs peuvent expliquer leur prédisposition à l’apparition d’un syndrome d’hypertension pulmonaire se compliquant d’ascite. Du fait d’un développement relativement plus lent de leur système cardio-respiratoire, certains animaux ont parfois des difficultés à assurer un approvisionnement correct de leurs tissus musculaires en oxygène. Leur capacité d’adaptation à une stimulation du métabolisme par des facteurs environnementaux (climat, altitude, alimentation riche en protéines et en énergie ) est limitée et favorise indirectement l’installation d’une hypoxie relative en augmentant les besoins en O2. Les généticiens recherchent de nouveaux critères de sélection sur les caractéristiques pulmonaires, hématologiques et cardio-vasculaires en équilibre avec les caractéristiques de croissance.
Hole Cleaning in Horizontal Wells Using Viscoelastic Fluids: An Experimental Study of Drilling-Fluid Properties on the Bed-Erosion Dynamics
Authors: Hirpa Mehmet Meric , Kuru Ergun 
Published: June 2020

Summary
An experimental study was conducted to determine the influence of fluid elastic properties on the critical velocity, frictional pressure drops, and the turbulent-flow characteristics of polymer-fluid flow over a sand bed deposited in a horizontal pipe. Fluids were prepared using a special technique, which allowed for the alteration of fluid elastic properties while keeping the shear viscosity constant. By conducting experiments under controlled conditions, we were able to quantify the individual effect of the fluid elasticity (independent from shear viscosity) on the critical flow rate for bed erosion and the turbulent-flow characteristics of polymer-fluid flow over the stationary sand bed. Results showed that higher critical velocities were required for the onset of the bed erosion when we use the fluid with higher elasticity.