bluuebunny/crossref_metadata_2025_split huggingface dataset:
The alteration of ­­­­­­metabolic profiling in the second trimester of GDM and high risk pregnancy, and a nomogram for predicting macrosomia
Authors: Sun Man , Zhao Baihui , He Sainan , Weng Ruopeng , Wang Binqiao , Ding Yunping , luo qiong 
Published: December 2019

Abstract
Objective Dyslipidemia in the second trimester and associated gestational diabetes are increasing worldwide. Carnitine plays a key role in lipid metabolism. We aim to describe metabolic profiling in the second trimester based on carnitine related metabolomics in GDM and high risk pregnancy, and to find the potential risk factors in GDM and candidate metabolites for diagnosing GDM induced macrosomia.Methods We have randomly investigated 450 pregnant women and their neonates in this retrospective study and 56 (12.4%) GDM cases were diagnosed. We used LC-MS/MS performing metabolic profiling about 12 amino acids and 31 acylcarnitines (containing C0) to assess circulating metabolites concentration in different subgroup according maternal and newborn clinical characteristic. We also calculated the correlation coefficient between maternal and newborn. GDM potential metabolic risk factors were screened by PLS-DA. Multivariate regression analyses were used in identifying independent risk factors for GDM and macrosomia. Based on these carnitine-related factors, a nomogram for estimating macrosomia was developed.Results We found 14 AA (Ala, Arg, Met, BCAA, AAA) and AC (C0, C2, C3, C4DC+C5OH, C5, C16, C18, C18:1) were increased in Age > 35 group, BMI ≥ 30, weight gain > 20 kg group, using assistant reproductive technology group, but the level of C0, Gly were decreased. In fetal clinical data, we obtained AA and AC level in fetuses are higher than their mothers and the metabolic trend was similar with maternal result. PLS-DA showed 15 metabolism(C0, LEU+ILE+PRO-OH, Phe, C18, TYR, etc)play main roles in class separation of GDM. Multivariate analysis showed pre-pregnancy BMI, weight gain, LEU+ILE+PRO-OH, TYR, C0/acylcarnitine, C0, C3, C16, C18 are independent risk factors associated with GDM. Finally, we developed a nomogram predicting macrosomia based on carnitine-related metabolic variables.Conclusion Metabolomics was proved as a powerful tool in identifying the metabolic alteration during the second trimester. These metabolic risk factors in GDM may help understanding of the underlying biochemical pathology of GDM and help physician diagnosing macrosomia.
Substantiation of Initial-I Stage of Generalized Periodontitis Complex Treatment Choice Among Medical University Students According to Indicators’ Dynamics Changes of Oral Cavity Prooxidant-antioxidant System
Authors: Pavliuk Tetiana , Rozhko Mykola , Panchak Oksana 
Published: December 2019

According to the results of epidemiological studies, the spread of periodontal tissue diseases has tendency to increase, despite recent decades’ significant achievements in dentistry. Studies of periodontal tissue diseases pathogenesis have found that oxygen-dependent production of reactive oxygen species (ROS) and antioxidant activity are involved in the pathogenic mechanism of the disease. ROS are highly toxic and can induce lipid peroxidation (LPO). Researchers have shown increased levels of LPO with generalized periodontitis (GP). "Noofen" is one of the drugs that normalizes the processes of LPO and shows positive effect in GP treatment. That is why in advanced complex treatment of generalized periodontitis of initial-I stage, we’ve used this pill.
The objective of the study was to analyze the efficiency of nootropic medicine "Noofen" usage in suggested initial-I stage of generalized periodontitis treatment, taking into account the dynamics of oral fluid prooxidant and antioxidant indicators among students with high level of emotional sensitivity.
Materials and methods. A study of 2nd year students (174) of Ivano-Frankivsk National Medical University were questioned in order to determine the level of individual emotional states (anxiety, frustration, aggression and rigidity) according to the method of H. Eysenck. Of those who have experienced increased and high level of emotional sensitivity to various stressors and situations, we have selected 50 students with initial-I stage of generalized periodontitis. To determine the efficiency of treatment, two groups were formed: observation (group I) and comparison (group II). Each group consisted of 25 individuals who were treated and monitored dynamically during the year. The condition of oral cavity prooxidant system was determined by the level of malondialdehyde (MDA) and diene conjugates (DC). To study the state of the antioxidant protection system, the activity of superoxide dismutase (SOD), glutathione-S-transferase (G-ST) and glutathione peroxidase (GPO) were determined. The advanced treatment, which included oral rinsing with "Octenisept" solution, dissolving of tablets "Lizak", intake of medicine "Noofen" and vitamin-microelement complex "Vita-Supradin Active".
Conclusions. The results of the performed treatment indicate that the use of advanced therapy of GP is significantly more effective, confirmed by prooxidant-antioxidant system parameters of oral fluid in the long term, compared with traditional treatment.
Effect of Pathological Gestational Weight Gain on Subsequent Lactation
Authors: Ostafiichuk Svitlana 
Published: December 2019

The onset, duration of lactation and exclusive breastfeeding (BF) depend on maternal factors. Objective: The aim of our study was to investigate the effect of pathological gestational weight gain (GWG) on subsequent lactation. Design: This was a prospective cohort study of 219 women, aged 18 to 43 years, who were enrolled at 9-11 weeks and were followed-up at 22-24, 37-39 weeks of pregnancy, and 1 year postpartum. Anthropometry, body mass index, GWG were calculated. The percentage of body fat mass (% FM) was calculated on the basis of bioelectrical impedance analysis (BIA) using the “Diamant-aist” analyzer (St. Petersburg). The delayed onset of lactation (more than 72 hours), the duration of lactation, exclusive BF were interviewed. The results were statistically analyzed using statistical analysis package based on Microsoft Excel and Statistica 6.0 program pack (StatSoft Inc., USA). Results: The total BF and exclusive BF median durations were 29.7±10.1 (95% Cl 25.7-33.7) weeks and 22.2±6.2 (95% Cl 18.2-26.2) weeks, respectively. A negative association between the elevation of the %FM during pregnancy and the duration of BF (r= - 0.21, p=0.001; r= - 0.32, p<0.0001; r=- 0.47, p<0.0001, respectively in the first, second and third trimesters) was diagnosed. It has been proved that excessive GWG was significantly associated with shorter duration of lactation (p<0.05), with shorter duration of exclusive BF (OR 0.5; 95% Cl 0.2-0.9, p=0.03), with increased chances of discontinuing BF before 6 month postpartum (OR 2.2; 95% Cl 1.0-4.5, p=0.04), and delayed onset of lactation (OR 2.1; 95% Cl 1.1-4.1, p=0.04) compared with the recommended GWG women. Conclusion: Informing the pregnant women about the negative effect of pathological GWG on subsequent lactation, regular assessing the dynamics of weight gain during pregnancy, and taking measures to prevent overweight and obesity in the postpartum period should be an important focus of antenatal care.
The Use of the Test for Oral Muscular Coordination Ability to Increase the Level of Adaptive Capacity of Oral Cavity Organs to Prosthodontic Treatment of Patients with Completely Absent Dentition
Authors: Sarapuk Volodymyr 
Published: December 2019

Increase in the level of adaptive capacity of oral cavity organs to the prosthodontic treatment of patients with completely absent dentition is one of the urgent problems of modern dentistry. In order to increase the level of adaptability to complete removable laminar dentures, a set of standard samples according to H. Landt was used to test the ability for precise coordination of masticatory muscles – the MA-test (Muscular-Ability) as a means of a group of muscles stimulation providing precise coordination of masticatory muscles. This adaptation process was called MA-stimulation. The level of adaptive capacity of the oral cavity organs was studied using oral stereognosis. The criteria for oral stereognosis assessment included the average time of one sample determination (sec) and the correctness of the samples determination (%). In order to achieve the objective, we conducted a study of tactile sensitivity in the oral cavity using oral stereognosis in 90 patients with completely absent dentition who applied for the prosthodontic retreatment. The patients’ age ranged from 45 to 89 years. The patients were divided into two groups. Group I included 30 patients who underwent the prosthodontic treatment without preliminary MA-stimulation. Group II included 60 patients who underwent MA-stimulation for 14 days before the prosthodontic treatment.
According to the results of oral stereognosis in Group II of patients, the average time of one sample determination after fourteen days of MA-stimulation decreased by 24.5% (p˂0.05) and the index of correctness of the samples determination increased by 32.0% (р˂0.001); the average time of one sample determination decreased by another 19.3% in 1 month after the prosthodontic treatment, and it decreased by 17.4% (р˂0.05) in 3 months after the prosthodontic treatment; the index of the correctness of the samples determination increased by 17.5% (p˂0.001) and 10.5% (p˂0.01) respectively in 1 and 3 months after the prosthodontic treatment.
Oral stereognosis indices changed significantly only in 1 month after the prosthodontic treatment in Group I of patients who did not undergo MA-stimulation: the average time of one sample determination decreased only by 14.7% (p˂0.05), and the index of correctness of the samples determination increased only by 24.5 (p˂0.05).
According to the results of the research, MA-stimulation is advisable to be included into the prosthodontic treatment of the patients with completely absent dentition as a means of increasing the adaptive capacity of the oral organs.
Effect of Methanolic Extract of Justicia Flava on Petroleum-Induced Reduced Cell Division and DNA Damage
Authors: Achuba Fidelis Ifeakachuku , Mordi Joseph C 
Published: December 2019

The objective of this research was to determine the import of Justicia flava methanolic extract against petroleum-stimulated reduced cell division and DNA fragmentation.
Materials and Methods. Ninety rats were distributed and treated as follows for thirty days: Group A - normal diet; Group B - normal feed + 100mg/kg bwt of Justicia flava methanolic extract; Group C - normal feed + 200mg/kg bwt of Justicia flava methanolic extract; Group D - normal feed + 300mg/kg bwt of Justicia flava methanolic extract; Group E - normal feed + 250 mg/kg bwt of standard ascorbic acid; Group F - crude oil-tainted feed; Group G - crude oil-tainted feed + 250 mg/kg bwt of standard ascorbic acid; Group H - crude oil-tainted feed + 100mg/kg bwt of Justicia flava methanolic extract; Group I - crude oil-tainted feed + 200mg/kg bwt of Justicia flava methanolic extract. After thirty days, the rats were sacrificed under chloroform sedation and the liver and kidney were collected for cellular analysis via standard protocols.
Results. The results showed that administration of Justicia flava methanolic extract decreased petroleum-induced reduced cell division and DNA damage in the liver and kidney of experimental rats. Justicia flava methanolic extract is potent antidote for petroleum-induced DNA damage in the liver and kidney of rats.
Conclusions. It is no doubt that ingestion of petroleum-tainted diet could culminate in DNA damage in organs and tissues of animals. However, the administration of Justicia flava methanolic extract was found to prevent this damage in renal and liver cells of experimental rats.
Possible Mechanism of Aframomum Sceptrum Extracts Mediated Modulation of Renal Function after Monosodium Glutamate Exposure
Authors: Ichipi-Ifukor Patrick Chukwuyenum , Ogbeke Geoffrey Ighowho , George Betty Omenebelle 
Published: December 2019

The objective of the research was to explain the possible mechanism of an earlier reported role of Aframomum sceptrum extract in the modulation of renal function parameters in monosodium glutamate-induced toxicity.
Materials and Methods. Similar experimental methods previously reported by us in Ogbeke et al., (2016) were maintained.
Results. Monosodium glutamate administration led to a significant elevation of levels of serum and kidney lipid peroxidation due to decrease in the levels of serum and kidney antioxidant enzyme, super oxide dismutase, catalase, gluthathione peroxidase and gluthathione. There was observed increase in oxidative enzyme, aldehyde oxidase, sulphite oxidase, xanthine oxidase and monoamine oxidase activities in serum and kidney after monosodium glutamate consumption. Aframomum sceptrum treatment significantly regulated all altered indices.
Conclusions. The study concluded that the ability of Aframomum sceptrum extract to modulate renal function parameters in monosodium glutamate-induced toxicity is dependent on its efficacy in the induction and mobilization of antioxidant defense armory via the increased synthesis of tissue and serum enzymatic and non-enzymatic antioxidants, as well as improved oxidative enzyme activities that mediates the quenching of rising aldehydes and sulfoxides, N-oxides and aromatic oxides within the kidney.
The Role of Insulin, IGF-1 and PRAS40 in the Processes of Oncogenesis in Women with Type 2 Diabetes Mellitus and Endometrial Cancer
Authors: Vatseba Tamara 
Published: December 2019

The objective of the research was to investigate the content of insulin, insulin-like growth factor-1 and phosphorylated protein kinase proline-rich Akt substrate of 40kDa and to determine their role in the activation of oncogenesis processes in women with type 2 diabetes mellitus and endometrial cancer.
Materials and methods. There were examined 46 women who were divided into 4 groups: Group I included healthy women; Group II comprised women with type 2 diabetes mellitus; Group III included women with endometrial cancer; Group IV comprised women with endometrial cancer and co-existent type 2 diabetes mellitus. The levels of insulin, insulin-like growth factor-1, phospho- proline-rich Akt substrate of 40kDa were determined by immune-enzyme analysis. The compensation of diabetes mellitus was evaluated by hemoglobin A1c level using method of ion-exchange chromatography. The results obtained were analyzed using statistical analysis.
Results. Women of all study groups had increased levels of insulin and insulin-like growth factor-1 as compared to the control group (p<0.05). The level of phospho-proline-rich Akt substrate of 40kDa increased in the patients of Group II (p<0.05) and the patients of Group III (p<0.05) and decreased in the patients of Group IV (p<0.05). According to correlation analysis, phospho-proline-rich Akt substrate of 40kDa was found to correlate with body mass index, insulin and insulin-like growth factor-1 in Group II, body mass index and insulin-like growth factor-1 in Group III and body mass index in Group IV (p<0.05).
Conclusions. There was found an association between type 2 diabetes mellitus and endometrial cancer through obesity, hyperinsulinemia and insulin-like growth factor-1. The increase in phospho- proline-rich Akt substrate of 40kDa level was a sign of activation of mTOR and oncogenesis processes in the patients with type 2 diabetes mellitus. The decrease in phospho-proline-rich Akt substrate of 40kDa in the patients with endometrial cancer and co-existent type 2 diabetes mellitus can be explained by the influence of other intracellular regulatory systems or the effects of antidiabetic drugs, that requires additional study.
Use of Notification and Communication Technology (Call Light Systems) in Nursing Homes: Observational Study
Authors: Ali Haneen , Li Huiyang 
Published: December 2019

Background
The call light system is one of the major communication technologies that link nursing home staff to the needs of residents. By providing residents the ability to request assistance, the system becomes an indispensable resource for patient-focused health care. However, little is known about how call light systems are being used in nursing homes and how the system contributes to safety and quality of care for seniors.

Objective
This study aimed to understand the experiences of nursing home staff who use call light systems and to uncover usability issues and challenges associated with the implemented systems.

Methods
A mix of 150 hours of hypothetico-deductive (unstructured) task analysis and 90 hours of standard procedure (structured) task analysis was conducted in 4 different nursing homes. The data collected included insights into the nursing home’s work system and the process of locating and responding to call lights.

Results
The data showed that the highest alarm rate is before and after mealtimes. The staff exceeded the administration’s expectations of time to respond 50% of the time. In addition, the staff canceled 10.0% (20/201) of call lights and did not immediately assist residents because of high workload. Furthermore, the staff forgot to come back to assist residents over 3% of the time. Usability issues such as broken parts, lack of feedback, lack of prioritization, and low or no discriminability also contributed to the long response time. More than 8% of the time, residents notified the staff about call lights after they waited for a long time, and eventually, these residents were left unattended.

Conclusions
Nursing homes that are still using old call light systems risk the continuation of usability issues that can affect the performance of the staff and contribute to declining staff and resident outcomes. By incorporating feedback from nurses, nursing home management will better understand the influence that the perceptions and usability of technology have on the quality of health care for their residents. In this study, it has been observed that the call light system is perceived to be an important factor affecting the outcomes of the care process and satisfaction of both residents and staff as well as the staff’s performance. It is important to recognize that communication and notification technology contributes to the challenges the staff faced during their work, making their working conditions more difficult and challenging.
Дослідження впливу клітинних технологій на розвиток остеоартрозу колінного суглоба в експерименті
Authors: Tkachuk P.V. , Strafun S.S. 
Published: December 2019

Актуальність. Для лікування прогресуючих дистрофічних змін колінного суглоба при остеоартрозі запропоновано використання автологічних клітинних матеріалів. Мета: дослідження структурних змін менісків та епіфіза ушкодженої остеоартрозом великогомілкової кістки після застосування клітинних препаратів. Матеріали та методи. В експериментах кроликам моделювали остеоартроз, через 1 місяць у порожнину колінного суглоба вводили тромбоцитарну плазму, автологічний аспірат кісткового мозку і гомогенізованої жирової тканини.Через 2,5 місяця після операції досліджували структурні зміни менісків і проксимального епіфіза великогомілкової кістки. Ушкодження та відновлення оцінювали за морфологічними критеріями: зміни структури меніска, ушкодження епіфізарного хряща великогомілкової кістки, зміни щільності субхондральної кісткової тканини. Результати. Аналіз гістологічних досліджень показав зменшення ушкодження хряща тіла менісків після введення всіх трьох препаратів, часткову зупинку зміни щільності кісткової тканини після застосування тромбоцитарної плазми й аспірату кісткового мозку, а прогресуючі дегенеративні зміни суглобової поверхні спостерігалися лише у групі з плазмою. Через 1,5 місяця після введення аспіратів, отриманих із кісткового мозку та жирової тканини, відбулась інтеграція введених клітин із синовіальною оболонкою та меніском: розвиток ретикулярної тканини у групі з аспіратом кісткового мозку, формування ніжної сполучної тканини з адипоцитами у групі з аспіратом жирової тканини. Висновки. Про життєздатність клітин уведених аспіратів свідчить реорганізація та їх часткова інтеграція у синовіальній порожнині, зокрема через активацію ангіогенезу.
Зміни метаболізму пуринів при кістково-суглобовій формі раку легені
Authors: Syniachenko O.V. , Aliiev R.F. , Iermolaieva M.V. , Bondar V.G. 
Published: December 2019

Актуальність. Актуальність проблеми набуває особливої значущості у зв’язку з почастішанням випадків метастазування раку легені (РЛ) у скелет, що істотно погіршує показники виживаності хворих. Ця так звана кістково-суглобова форма РЛ спостерігається в 30–40 % випадків захворювання. Пуринові основи належать до біомаркерів кісткових метастазів при РЛ, але їх діагностична та прогностична роль не вивчена. Мета: визначити стан пуринового метаболізму при кістково-суглобовій формі РЛ з оцінкою клініко-патогенетичної значущості виявлених змін. Матеріали та методи. Обстежено 123 хворих (усі чоловіки) віком від 24 до 79 років. Співвідношення периферичної і центральної форм раку становило 1 : 4, дрібноклітинного та недрібноклітинного гістологічного варіанта — 1 : 4, а IВ, IIА, IIВ, IIIА, IIIВ та IV стадій захворювання — 1 : 2 : 4 : 6 : 5 : 6. Кількість хворих із метастазами в лімфатичні вузли та віддалені органи становила 89 і 62 % відповідно. У сироватці крові вивчали рівні продуктів пуринового метаболізму — сечової кислоти, оксипуринолу, аденіну, гуаніну, ксантину, гіпоксантину, активності ксантиноксидази, ксантиндезамінази, аденозиндезамінази та 5-нуклеотидази. Результати. Дисметаболізм пуринів спостерігається у 98 % від числа хворих на РЛ, що проявляється підвищенням концентрації у крові сечової кислоти й оксипуринолу, активності ксантиноксидази й аденозиндезамінази. Показники пуринового обміну при РЛ відбивають ступінь тяжкості перебігу захворювання, пов’язані з локалізацією та морфологічною формою пухлинного процесу, проростанням у грудну стінку, трахею та стравохід, наявністю компресійного синдрому й обтураційного ателектазу. Кістково-суглобову форму РЛ встановлено у 32 % від числа хворих на РЛ, що супроводжується збільшенням показників урикемії, оксипуринолемії й аденінемії, що беруть участь в патогенетичних побудовах пухлинного процесу. Рівень аденіну (6-амінопурину) щодо метастазів РЛ у скелет має прогнознегативну значущість. Виживаність хворих з кістково-суглобовою формою РЛ гірша, ніж у інших пацієнтів.