bluuebunny/crossref_metadata_2025_split huggingface dataset:
Breed Differences in Dog Cognition Associated with Brain-Expressed Genes and Neurological Functions
Authors: Gnanadesikan Gitanjali E , Hare Brian , Snyder-Mackler Noah , Call Josep , Kaminski Juliane , Miklósi Ádám , MacLean Evan L 
Published: July 2020

SynopsisGiven their remarkable phenotypic diversity, dogs present a unique opportunity for investigating the genetic bases of cognitive and behavioral traits. Our previous work demonstrated that genetic relatedness among breeds accounts for a substantial portion of variation in dog cognition. Here, we investigated the genetic architecture of breed differences in cognition, seeking to identify genes that contribute to variation in cognitive phenotypes. To do so, we combined cognitive data from the citizen science project Dognition.com with published breed-average genetic polymorphism data, resulting in a dataset of 1654 individuals with cognitive phenotypes representing 49 breeds. We conducted a breed-average genome-wide association study to identify specific polymorphisms associated with breed differences in inhibitory control, communication, memory, and physical reasoning. We found five single nucleotide polymorphisms (SNPs) that reached genome-wide significance after Bonferroni correction, located in EML1, OR52E2, HS3ST5, a U6 spliceosomal RNA, and a long noncoding RNA. When we combined results across multiple SNPs within the same gene, we identified 188 genes implicated in breed differences in cognition. This gene set included more genes than expected by chance that were (1) differentially expressed in brain tissue and (2) involved in nervous system functions including peripheral nervous system development, Wnt signaling, presynapse assembly, and synaptic vesicle exocytosis. These results advance our understanding of the genetic underpinnings of complex cognitive phenotypes and identify specific genetic variants for further research.
Simultaneous Viral Whole-Genome Sequencing and Differential Expression Profiling in Respiratory Syncytial Virus Infection of Infants
Authors: Lin Gu-Lung , Golubchik Tanya , Drysdale Simon , O’Connor Daniel , Jefferies Kimberley , Brown Anthony , de Cesare Mariateresa , Bonsall David , Ansari M Azim , Aerssens Jeroen , Bont Louis , Openshaw Peter , Martinón-Torres Federico , Bowden Rory , Pollard Andrew J ,   RESCEU Investigators, Nair Harish , Campbell Harry , Cunningham Steve , Beutels Philippe , Bont Louis , Wildenbeest Joanne , Pollard Andrew , Butler Christopher , Snape Matthew , Drysdale Simon , Lin Gu-Lung , O’Connor Daniel , Clutterbuck Elizabeth , Jefferies Kimberley , McGinley Joseph , Openshaw Peter , Thwaites Ryan , Wiseman Dexter , Martinon-Torres Federico , Gómez-Carballa Alberto , Heikkinen Terho , Meijer Adam , Fischer Thea Kølsen , van den Berge Maarten , Giaquinto Carlo , Abram Michael , Swanson Kena , Leach Amanda , Demont Clarisse , Gallichan Scott , Aerssens Jeroen , Öner Deniz , Rosen Brian , Molero Eva , Nair Harish , Campbell Harry , Cunningham Steve , Beutels Philippe , Bont Louis , Wildenbeest Joanne , Pollard Andrew , Butler Christopher , Snape Matthew , Drysdale Simon , Lin Gu-Lung , O’Connor Daniel , Clutterbuck Elizabeth , Jefferies Kimberley , McGinley Joseph , Openshaw Peter , Thwaites Ryan , Wiseman Dexter , Martinon-Torres Federico , Gómez-Carballa Alberto , Heikkinen Terho , Meijer Adam , Fischer Thea Kølsen , van den Berge Maarten , Giaquinto Carlo , Abram Michael , Swanson Kena , Leach Amanda , Demont Clarisse , Gallichan Scott , Aerssens Jeroen , Öner Deniz , Rosen Brian , Molero Eva 
Published: July 2020

Abstract
Targeted metagenomics using strand-specific libraries with target enrichment is a sensitive, generalized approach to pathogen sequencing and transcriptome profiling. Using this method, we recovered 13 (76%) complete human respiratory syncytial virus (RSV) genomes from 17 clinical respiratory samples, reconstructed the phylogeny of the infecting viruses, and detected differential gene expression between 2 RSV subgroups, specifically, a lower expression of the P gene and a higher expression of the M2 gene in RSV-A than in RSV-B. This methodology can help to relate viral genetics to clinical phenotype and facilitate ongoing population-level RSV surveillance and vaccine development.
Clinical Trials Registration. NCT03627572 and NCT03756766.
Intermittent micafungin for prophylaxis in a rat model of chronic Candida albicans gut colonization
Authors: Warn Peter , Thommes Pia , Sharp Andrew , Sattar Abdul , Undre Nasrullah , Palacios-Fabrega Jose Alejandro , Karas Andreas 
Published: July 2020

AbstractBackgroundDuring antifungal prophylaxis, micafungin is generally infused IV once daily over 1 h. In practice, less-frequent dosing could improve the quality of life in patients requiring long-term treatment or prophylaxis. The feasibility of this approach was assessed using humanized doses of daily or infrequent micafungin regimens.ObjectivesTo evaluate the effectiveness of intermittent high-dose micafungin, simulating human exposure, for prophylaxis of invasive candidiasis in a rat model of chronic Candida albicans gastrointestinal colonization and systemic dissemination.MethodsTwo weeks post-infection with an oral challenge of C. albicans, Sprague–Dawley rats were immunocompromised with a cytotoxic drug and a steroid. Rats received IV infusions of: daily vehicle control; daily subcutaneous micafungin (20 mg/kg SC); high-dose micafungin (20 mg/kg bolus SC + 80 mg/kg infusion/72 h, to simulate intermittent human dosing of 300 mg/72 h); or daily fluconazole by mouth (10 mg/kg PO). The effects of antifungal prophylaxis on faecal fungal burden and systemic C. albicans dissemination were evaluated.ResultsA rat model of chronic C. albicans gastrointestinal colonization and systemic dissemination was established, characterized by a sustained microbiological burden over 29 days and fungal recovery from normally sterile tissues. Using this model, intermittent high-dose micafungin (delivered via iPrecio pumps) to simulate humanized doses of 300 mg/72 h was significantly more effective than vehicle control, as effective as once-daily micafungin and similar to daily fluconazole at reducing faecal burden and preventing systemic dissemination.ConclusionsThese data indicate that intermittent high-dose micafungin can be as effective as daily therapy, supporting clinical assessment in high-risk patients requiring long-term antifungal prophylaxis.
Supplementation frequency and amount modulate postweaning growth and reproductive performance of Bos indicus-influenced beef heifers
Authors: Moriel Philipe , Palmer Elizabeth , Vedovatto Marcelo , Piccolo Matheus B , Ranches Juliana , Silva Hiran Marcelo , Mercadante Vitor R G , Lamb G Cliff , Vendramini Joao M B 
Published: July 2020

Abstract
This 2-yr study evaluated the growth and puberty attainment of Bos indicus-influenced beef heifers offered 2 different postweaning concentrate supplementation amounts and delivery frequencies. On day 0 of each year, 64 Brangus crossbred heifers were stratified by initial body weight (BW) and age (mean = 244 ± 22 kg; 314 ± 17 d) and assigned into 1 of 16 bahiagrass pastures (4 heifers/pasture/yr). Treatments were randomly assigned to pastures in a 2 × 2 factorial design (4 pastures/treatment/yr) and consisted of concentrate dry matter (DM) supplementation at 1.25% or 1.75% of BW which were offered either daily (7×) or 3 times weekly (3×) for 168 d. On day 56 of each year, heifers were assigned to an estrus synchronization protocol consisting of intravaginal controlled internal drug release (CIDR) insertion on day 56, CIDR removal on day 70, i.m. injection of 25 mg of prostaglandin F2α (PGF2α) on day 86, and i.m. injection of 100 µg of gonadotropin-releasing hormone and timed-AI at 66 h after PGF2α injection (day 89). Heifers were exposed to Angus bulls from day 89 to 168 (1 bull/pasture). Pregnancy diagnosis was assessed on day 213 of each year. Supplementation amount × frequency effects were not detected (P ≥ 0.12) for any variable, except for plasma concentrations of glucose (P = 0.10) and urea nitrogen (PUN; P = 0.01). Herbage mass, herbage allowance, and nutritive value did not differ (P ≥ 0.12) among treatments. Increasing supplementation DM amount from 1.25% to 1.75% of BW increased (P ≤ 0.05) plasma concentrations of insulin-like growth factor 1 (IGF-1), overall average daily gain (ADG), final BW, percentage of pubertal heifers on day 89, pregnancy and calving percentages, and percentage of heifers calving within the first 21 d of the calving season. However, reducing the supplementation frequency from daily to 3× weekly, regardless of supplementation amount, did not impact overall pregnancy and calving percentages (P ≥ 0.42), but caused (P ≤ 0.05) fluctuations in plasma concentrations of insulin and IGF-1 and decreased (P ≤ 0.03) overall ADG, final BW, puberty attainment on days 56, 89, and 168, and percentage of heifers calving during the first 21 d of the calving season. Hence, increasing the supplement DM amount did not prevent the negative effects of reducing the frequency of supplementation (3× vs. 7× weekly) on growth and reproduction of replacement Bos indicus-influenced beef heifers.
Collaboration, Collegiality, and Sharing of Resources: Benefits of a Visiting Professor Exchange Program in Breast Imaging
Authors: Shah Biren A , Jeon Janice Y , Crane Erin P , Baffoe Nana Ama , Joseph Bobby 
Published: July 2020

Abstract
The role of a visiting professor (VP) has long been used as a model to share medical knowledge and new advances between different teaching institutions. As with all subspecialties, breast imaging has institution-specific differences in resources and faculty. Sharing of resources between programs can have a profound impact on enriching educational experiences for learners. Our conceptual design of a VP lecture exchange program was between two academic medical centers, Virginia Commonwealth University Health System (VCU) and MedStar Georgetown University Hospital University (MGUH), in the subspecialty of breast imaging. The program was designed to supplement potential areas of weakness in the breast imaging educational curriculum at each respective institution. The program also sought to create opportunities for long-term mentorship and collaboration between institutions. Three faculty members from VCU and three faculty members from MGUH participated for a total of six lectures (three lectures at each site). Participating residents and faculty completed anonymous surveys following each lecture regarding the lecture exchange program experience. The survey responses showed that the VP exchange was well received at both institutions. The VP exchange process was relatively easy to arrange, benefits both institutions, and could even be expanded to the virtual environment.
Field Perimeter Trapping to Manage Rhagoletis mendax (Diptera: Tephritidae) in Wild Blueberry
Authors: Drummond Francis A , Collins Judith A 
Published: July 2020

Abstract
Rhagoletis mendax Curran (the blueberry maggot fly) is a major pest of wild blueberry. It is a direct pest of the fruit. Females lay eggs in fruit resulting in infestations of larvae unacceptable to most consumers. Three field perimeter interception tactics were tested for control of R. mendax in wild blueberry, Vaccinium angustifolium Aiton (Ericales: Ericaceae), between 2000 and 2010. We investigated field perimeter deployment of baited, insecticide-treated, green spheres (2000–2005), baited, yellow Trécé PHEROCON AM traps (2005 and 2006), and baited, Hopper Finder, sticky barrier tape (2008–2010). Only the Hopper Finder tape provided significant reduction in R. mendax adults and fruit infestation over the 3-yr field study. However, the reduction in fruit infestation compared with control plots was only 48.2 ± 7.3%, a level of reduction in damage that would be unsuitable for many commercial wild blueberry growers as a stand-alone tactic, but could be an important reduction as part of a multiple tactic IPM strategy. In addition, we constructed an agent-based computer simulation model to assess optimal trap placement between three patterns: 1) a single row of traps along field perimeter; 2) a double row of traps along the field perimeter at half the density of the single row; and 3) a grid of traps spread throughout the field but with the largest distance between traps. We found that the single row deployment pattern of traps was the best for reducing immigration of R. mendax adults into simulated fields.
Demography of Thrips palmi (Thysanoptera: Thripidae) Reared on Brassica oleracea (Brassicales: Brassicaceae) and Phaseolus vulgaris (Fabales: Fabaceae) With Discussion on the Application of the Bootstrap Technique in Life Table Research
Authors: Yang Xiaomin , Sun Lijuan , Chi Hsin , Kang Guodong , Zheng Changying 
Published: July 2020

AbstractIn order to contrast the fitness of Thrips palmi Karny reared on two of its hosts plants, Brassica oleracea L. leaves and Phaseolus vulgaris L. beans, life tables were constructed for T. palmi individuals reared on the two hostplants at 25°C using the age–stage, two-sex life table. The durations of the egg, first instar larva, pupa, and total immature stages of T. palmi reared on P. vulgaris were significantly longer than those reared on B. oleracea, whereas the adult preoviposition period, female adult longevity, and number of oviposition days of T. palmi reared on B. oleracea were significantly longer/greater than those on P. vulgaris. The fecundity (F) of T. palmi reared on B. oleracea (159.71 offspring/female) was significantly higher than in females reared on P. vulgaris (F = 122.98 offspring/female) despite the higher proportion of female adults that emerged on P. vulgaris. Although there were no significant differences in the intrinsic rate of increase (r), finite rate (λ), net reproductive rate (R0), and mean generation time (T) between the two populations, computer projection showed that the T. palmi population will increase faster when reared on B. oleracea than on P. vulgaris. Our results demonstrate that population projection based on life table data is potentially an important tool in pest management. Furthermore, we used the bootstrap technique to demonstrate the variable effect that survival rate and fecundity at different ages can have on population parameters.
The Effect of Simulated Lepidopteran Ear Feeding Injury on Mycotoxin Accumulation in Grain Corn (Poales: Poaceae)
Authors: Farhan Yasmine , Smith Jocelyn L , Limay-Rios Victor , Schaafsma Arthur W 
Published: July 2020

Abstract
Fusarium graminearum Schwabe (Hypocreales: Nectriaceae) and Fusarium verticillioides (Saccardo) (Hypocreales: Nectriaceae) Nirenberg infection results in accumulation of deoxynivalenol (DON), zearalenone (ZON), and fumonisin (FBs) mycotoxins in infected corn, Zea mays L. Lepidopteran insect feeding may exacerbate fungal infection by providing entry points on the ear resulting in increased mycotoxin contamination of grain. The objective of the current study was to simulate different types and severity levels (extent of injury) of lepidopteran injury to corn ears at different stages of ear development and its effect on mycotoxin accumulation in grain corn. Field experiments were conducted under conditions favorable for F. graminearum development where insect injury was simulated to corn ears and inoculated with F. graminearum. All simulated injury treatments resulted in elevated mycotoxin concentration compared with ears without simulated injury; however, the severity of injury within a treatment had little effect. Injury to kernels on the side of the ear resulted in greater DON and ZON concentration than injury to tip kernels, grazing injury applied at physiological maturity, or when no injury was simulated. Greater FBs was measured when tip kernel injury was simulated at the blister stage or when side kernel injury was simulated at milk and dent stages compared with noninjured ears, silk clipping, tip injury at milk and dent stages, or grazing injury at physiological maturity. The current study confirms that the risk of mycotoxin accumulation in the Great Lakes region is greater in the presence of ear-feeding insect pests and may differ depending on the feeding behavior of pest species.
Can the Addition of Odor and Visual Targets Enhance Attraction of the Asian Citrus Psyllid (Hemiptera: Liviidae) to Sticky Traps?
Authors: Khadka Arjun , Allan Sandra A , Cho Daniel , Weeks Emma N I 
Published: July 2020

Abstract
Asian citrus psyllid, Diaphorina citri Kuwayama, negatively impacts the citrus industry as it transmits Candidatus Liberibacter spp., the causal agent of citrus greening. Monitoring D. citri population levels is critical for management of vectors and citrus greening and is usually through use of yellow sticky traps. In our study, use of odors, odor blends, and visual targets were evaluated to determine whether attraction to yellow sticky traps could be improved. Methyl salicylate consistently increased D. citri attraction to decoy yellow but not to other yellow or yellow/green traps. Addition of a visual target did not enhance attraction to sticky traps. While several chemical blends were evaluated, they did not increase psyllid attraction to decoy yellow traps. The increased attraction to traps with methyl salicylate is promising and may contribute to trapping efficiency under field conditions.
In the Name of Legitimacy and Efficiency: Evaluating China’s Legal Reform on EIA
Authors: He Xiangbai 
Published: July 2020

Abstract
The environmental impact assessment (EIA) system is currently located at the centre of China's environmental legal reform. The Chinese government has undertaken significant legal strategies to rectify past regulatory wrongs, close enforcement gaps, and improve public participation. However, ongoing administrative reform and prevailing policies optimizing the business environment have made the efficacy of EIA reform more complicated and uncertain. Through examining how EIA laws and regulations on accountability, compliance, and participation have changed, this article provides an analytical framework to evaluate current legal reform of EIA. It finds that the Chinese government is attempting to bring about genuinely functional EIA reform by improving the legitimacy and efficiency of EIA in delivering better environmental decisions. Yet, its efforts could be undermined by the unsettled conflict between legitimacy and efficiency. Furthermore, functional EIA reform could be constrained by peripheral challenges such as inconsistent institutional arrangements, the undefined role of the judiciary, and an underrepresented environmental public interest.
Selection of Reference Genes for Quantitative Real-Time PCR in Chrysoperla nipponensis (Neuroptera: Chrysopidae) Under Tissues in Reproduction and Diapause
Authors: Wang Xiao , Kong Xue , Liu Shaoye , Huang Haiyi , Chen Zhenzhen , Xu Yongyu 
Published: July 2020

Abstract
Chrysoperla nipponensis (Okamoto), which has the unique diapause phenotype distinguishable from nondiapause adult, is an ideal model organism for studying the mechanism of reproductive diapause. However, there is no reliable and effective reference genes used for the reproductive diapause study of C. nipponensis. Therefore, in this study, we evaluated the expression stability of 10 candidate reference genes (Tub1, Arpc5, EF1a, 128up, RpS5, RpS26e, GAPDH, Arp3, Actin, α-Tub) in adults under diapause and nondiapause induction conditions using four statistical algorithms including GeNorm, NormFinder, Bestkeeper, and ∆CT method. Results showed that Arp3 and Tub1 were the most stable reference genes in all samples and in the adult tissues group. Arp3 and RpS5 were the most stable reference genes in the development degree group. α-Tub and EF1a were unstable reference genes under the conditions of this study. Meanwhile, to verify the reliability of the reference genes, we evaluated the relative expression levels of Vg and VgR in different treatments. Significant upregulation and downregulation in expression level of two genes in response to diapause termination and diapause fat body tissue was, respectively, observed when using Arp3 as the reference gene but not when using an unstable reference gene. The reference genes identified in this work provided not only the basis for future functional genomics research in diapause of C. nipponensis and will also identify reliable normalization factors for real-time quantitative real-time polymerase chain reaction data for other related insects.
The Governance of Foundation-Owned Firms
Authors: Hansmann Henry , Thomsen Steen 
Published: July 2020

Abstract
The burgeoning literature on corporate governance, both in economics and in law, has focused heavily on the agency costs of delegated management. It is therefore striking to encounter a large number of well-established and highly successful companies that have long been under the complete control of a self-appointing board of directors whose compensation is divorced from the profitability of the company and who cannot be removed or replaced by anyone except themselves.
The companies in question are those controlled by “industrial foundations,” which are nonprofit entities that possess a controlling interest in an otherwise conventional business corporation. Although common throughout Northern Europe, industrial foundations are particularly numerous in Denmark, where they control a quarter of the country’s 100 largest corporations. We work with a data set of 110 foundation-owned Danish firms to explore whether, and how, the governance structure of industrial foundations helps explain the strong performance of the firms they control. Given the absence of substantial material incentives, we concentrate on governance structures. We find a strong and robust relationship between the structure of foundation governance and firm performance. These results reinforce the view that, with the proper governance structure, pure fiduciaries can perform more efficiently than conventional economic models would predict. More specifically, these results underline the potential importance of the legislation that, in 2018, removed the long-standing barrier to forming industrial foundations in the USA.
Response to Commentaries
Authors: Beauchamp Tom L , Childress James F 
Published: July 2020

AbstractAfter expressing our gratitude to the commentators for their valuable analyses and assessments of Principles of Biomedical Ethics, we respond to several particular critiques raised by the commentators under the following rubrics: the compatibility of different sets of principles and rules; challenges to the principle of respect for autonomy; connecting principles to cases and resolving their conflicts; the value of and compatibility of virtues and principles; common morality theory; and moral status. We point to areas where we see common agreement with our commentators and respond to their critical evaluations.
Spin-down rate of the transitional millisecond pulsar PSR J1023+0038 in the optical band with Aqueye+
Authors: Burtovoi Aleksandr , Zampieri Luca , Fiori Michele , Naletto Giampiero , Spolon Alessia , Barbieri Cesare , Papitto Alessandro , Ambrosino Filippo 
Published: July 2020

ABSTRACT
We present a timing analysis of the transitional millisecond pulsar PSR J1023+0038 using observations taken between 2018 January and 2020 January with the high time resolution photon counter Aqueye+ mounted at the 1.82 m Copernicus telescope in Asiago. We report the first measurement of the timing solution and the frequency derivative of PSR J1023+0038 based entirely on optical data. The spin-down rate of the pulsar is (−2.53 ± 0.04) × 10−15 Hz2, which is ∼20 per cent slower than that measured from the X-ray observations taken in 2013–2016 and ∼5 per cent faster than that measured in the radio band during the rotation-powered state.