bluuebunny/crossref_metadata_2025_split huggingface dataset:
Varus alignment of the proximal tibia is associated with structural progression in early to moderate varus osteoarthritis of the knee
Authors: Palmer Jonathan S. , Jones Luke D. , Monk A. Paul , Nevitt Michael , Lynch John , Beard David J. , Javaid M. K. , Price Andrew J. 
Published: January 2020

Abstract
Purpose
Lower limb malalignment is a strong predictor of progression in knee osteoarthritis. The purpose of this study is to identify the individual alignment variables that predict progression in early to moderate osteoarthritis of the knee.

Method

A longitudinal cohort study using data from the Osteoarthritis Initiative. In
total, 955 individuals (1329 knees) with early to moderate osteoarthritis (Kellgren-Lawrence grade 1, 2 or 3) were identified. All subjects had full-limb radiographs
analysed using the Osteotomy module within Medicad® Classic (Hectec GMBH) to
give a series of individual alignment variables relevant to the coronal alignment of the
lower limb. Logistic regression models, with generalised estimating equations were
used to identify which of these individual alignment variables predict symptom
worsening (WOMAC score > 9 points) and or structural progression (joint space
narrowing progression in the medial compartment > 0.7mm) over 24 months.

Results
Individual alignment variable were associated with both valgus and varus
alignment (mechanical Lateral Distal Femoral Angle, Medial Proximal Tibial Angle and
mechanical Lateral Distal Tibial Angle). Only the Medial Proximal Tibial Angle was
significantly associated with structural progression and none of the variables was
associated with symptom progression. The odds of joint space narrowing progression
in the medial compartment occurring at 24 months increased by 21% for every one
degree decrease (more varus) in Medial Proximal Tibial Angle (p < 0.001)

Conclusions
Our results suggest that the risk of structural progression in the medial
compartment is associated with greater varus alignment of the proximal tibia.

Level of evidence
Level III, retrospective cohort study.
Study of optical and dielectric constants of hybrid SnO2 electrospun nanostructures
Authors: Matysiak Wiktor , Tański Tomasz , Smok Weronika 
Published: January 2020

AbstractThe aim of this paper was to prepare SnO2 nanowires using electrospinning and calcination processes from a poly(vinylpyrrolidone), dimethylformamide, ethanol and tin(IV) chloride pentahydrate solution. The composite PVP/SnCl4 nanofibers obtained via electrospinning method were dried and calcined in a vacuum to remove the polymer matrix at a temperature of 500 °C for 10 h. Three types of nanowires with a polymer to precursor ratios of 2:1, 1:1, 1:3 were produced. The morphology and chemical composition of as-spun PVP/SnCl4 nanofibers and SnO2 nanowires obtained after heat treatment were carried out using a scanning electron microscope (SEM) with an energy-dispersive spectrometer (EDX). The Fourier-transform infrared spectroscopy (FTIR) spectra of the prepared nanomaterials were also investigated. To determine the topography of PVP/SnCl4 nanofibrous mats, an atomic force microscope (AFM) was used. A 100-fold measurement of the nanowire size showed that, depending on the amount of precursor in the spinning solution, nanowires with diameters ranging from 20 to 260 nm were obtained. The optical property analysis was performed on the basis of absorbance spectra recorded over UV–Vis spectral range. The complex refractive index n and complex dielectric permeability ε of obtained tin oxide nanowires were determined as a function of the radiation energy. Depending on the precursor content in spinning solution, the one-dimensional SnO2 nanostructures were characterised by a refractive index in the range of 1.51–1.56, whereas the dielectric constant ranged from 2.26 to 2.30. The optical properties and the structure of one-dimensional SnO2 nanomaterials allow to use this type of materials in the construction of novel type photovoltaic cells and electronic devices.
Reference-free digital shadowgraphy using a moving BOS background
Authors: Gardner A. D. , Raffel M. , Schwarz C. , Braukmann J. N. , Wolf C. C. 
Published: January 2020

Abstract
A method of measuring density second derivative (shadowgraph) as a variant of background oriented schlieren (BOS) is presented. Pairs of images of a moving background with a small time difference are analysed using moving-window cross-correlation, giving the second derivative of density in the axis of background travel. The advantage over standard BOS is that no reference image is required. In contrast to the existing “reference-free BOS” method, no double-image is produced, making the data interpretation simpler. The sum-of-correlation (ensemble correlation) method can be used on an image series, where the flow is constant over the series time, to significantly reduce the size of the cross-correlation window. The reduction in the window size and sharpening of the resulting image is similar to that achieved by optical flow, but this method is easily automatable, without knowledge of the flow, and can be used with any existing BOS analysis software.

Graphic abstract
Neurons and astrocytes of the chicken hypothalamus directly respond to lipopolysaccharide and chicken interleukin-6
Authors: Grabbe Niklas , Kaspers Bernd , Ott Daniela , Murgott Jolanta , Gerstberger Rüdiger , Roth Joachim 
Published: January 2020

AbstractIn 4–5-month-old chicken, intravenous injections of bacterial lipopolysaccharide (LPS) induced a dose-dependent fever response and a pronounced increase of circulating interleukin-6 (IL-6). To assess a possible role for IL-6 in the brain of birds, a hypothalamic neuro-glial primary culture from 1-day-old chicken was established. Each well of cultured hypothalamic cells contained some 615 neurons, 1350 astrocytes, and 580 microglial cells on average. Incubation of chicken hypothalamic primary cultures with 10 or 100 µg/ml LPS induced a dose-dependent release of bioactive IL-6 into the supernatant. Populations of hypothalamic neurons (4%) and astrocytes (12%) directly responded to superfusion with buffer containing 10 µg/ml LPS with a transient increase of intracellular calcium, a sign of direct cellular activation. Stimulation of hypothalamic cultures with buffer containing 50 ng/ml chicken IL-6 induced calcium signaling in 11% of neurons and 22% of astrocytes investigated. These results demonstrate that IL-6 is produced in the periphery and in the hypothalamus in response to LPS in chicken. The observed cellular responses of hypothalamic cells to chicken IL-6 indicate that this cytokine may readily be involved in the manifestation of fever in the avian hypothalamus.
FISH and chips: a review of microfluidic platforms for FISH analysis
Authors: Rodriguez-Mateos Pablo , Azevedo Nuno Filipe , Almeida Carina , Pamme Nicole 
Published: January 2020

AbstractFluorescence in situ hybridization (FISH) allows visualization of specific nucleic acid sequences within an intact cell or a tissue section. It is based on molecular recognition between a fluorescently labeled probe that penetrates the cell membrane of a fixed but intact sample and hybridizes to a nucleic acid sequence of interest within the cell, rendering a measurable signal. FISH has been applied to, for example, gene mapping, diagnosis of chromosomal aberrations and identification of pathogens in complex samples as well as detailed studies of cellular structure and function. However, FISH protocols are complex, they comprise of many fixation, incubation and washing steps involving a range of solvents and temperatures and are, thus, generally time consuming and labor intensive. The complexity of the process, the relatively high-priced fluorescent probes and the fairly high-end microscopy needed for readout render the whole process costly and have limited wider uptake of this powerful technique. In recent years, there have been attempts to transfer FISH assay protocols onto microfluidic lab-on-a-chip platforms, which reduces the required amount of sample and reagents, shortens incubation times and, thus, time to complete the protocol, and finally has the potential for automating the process. Here, we review the wide variety of approaches for lab-on-chip-based FISH that have been demonstrated at proof-of-concept stage, ranging from FISH analysis of immobilized cell layers, and cells trapped in arrays, to FISH on tissue slices. Some researchers have aimed to develop simple devices that interface with existing equipment and workflows, whilst others have aimed to integrate the entire FISH protocol into a fully autonomous FISH on-chip system. Whilst the technical possibilities for FISH on-chip are clearly demonstrated, only a small number of approaches have so far been converted into off-the-shelf products for wider use beyond the research laboratory.
How images combine meaning: quantum entanglement in visual perception
Authors: Arguëlles Jonito Aerts , Sozzo Sandro 
Published: January 2020

AbstractVarious empirical tests performed on human participants and also by means of search engines on the Web reveal that, whenever the conceptual combination The Animal Acts is considered as a combination of the individual concepts Animal and Acts, the ‘Clauser–Horne–Shimony–Holt’ version of Bell’s inequalities (‘CHSH inequality’) is violated. We work out in this paper a quantum representation in Hilbert space for a dataset collected on the same combination of concepts using ‘Google Images’ as search engine, which ‘significantly violated’ the CHSH inequality. This result proves the existence of non-classical structures in visual perception and strongly indicates the presence of ‘quantum entanglement’ as an explanation for the meaning connection between the component concepts, also when this meaning connection is expressed through images.
Optimising Evidence-Based Psychological Treatment for the Mental Health Needs of Children with Epilepsy: Principles and Methods
Authors:   MICE Research Study Team, Shafran Roz , Bennett Sophie , Coughtrey Anna , Welch Alice , Walji Fahreen , Cross J. Helen , Heyman Isobel , Sibelli Alice , Smith Jessica , Ross Jamie , Dalrymple Emma , Varadkar Sophia , Moss-Morris Rona 
Published: January 2020

AbstractThere are potent evidence-based psychological treatments for youth with mental health needs, yet they are rarely implemented in clinical practice, especially for youth with mental health disorders in the context of chronic physical illness such as epilepsy. Implementation science, the study of the translation of research into practice, can promote the uptake of existing effective interventions in routine clinical practice and aid the sustainable integration of psychological treatments with routine health care. The aim of this report was to use four implementation science methods to develop a version of an existing effective psychological treatment for mental health disorders [the Modular Approach to Treatment of Children with Anxiety, Depression or Conduct Problems (MATCH-ADTC)] for use within paediatric epilepsy services: (a) literature search; (b) iterative focus groups underpinned by normalisation process theory; (c) Plan–Do–Study–Act methods; and (d) qualitative patient interviews. Findings: Three modifications were deemed necessary to facilitate implementation in children with both mental health disorders and epilepsy. These were (a) a universal brief psychoeducational component addressing the relationship between epilepsy and mental health; (b) supplementary, conditionally activated interventions addressing stigma, parental mental health and the transition to adulthood; and (c) additional training and supervision. The intervention needed relatively little alteration for implementation in paediatric epilepsy services. The modified treatment reflected the scientific literature and the views of clinicians and service users. The multi-method approach used in this report can serve as a model for implementation of evidence-based psychological treatments for children with mental health needs in the context of other chronic illnesses.
The resurgence of everyday experiences in school science learning activities
Authors: Kervinen Anttoni , Roth Wolff-Michael , Juuti Kalle , Uitto Anna 
Published: January 2020

AbstractScience education can be alienating for students, as it is apart from the mundane world with which they are familiar. Science education research has approached the gap between everyday understandings and science learning largely as a challenge arising while learning about science concepts and the kinds of instructional approaches that may support this. However, the forms of everyday ways of relating to the world fundamentally expand beyond conceptual understandings. In this study, we use data from an outdoor science learning setting to examine a range of non-conceptual but culturally possible and intelligible ways in which students actually connect science learning processes to their everyday world and its characteristic commonsense understandings. Our study shows how students’ (a) spontaneous embodied explorations, (b) humor in all of its bodily and grotesque forms, and (c) narrative representation and interpretation of the world are used to contextualize science learning, namely its environment and content, within their familiar world. We show how students draw on these fundamental cultural forms of understanding the world even without particular instructional support while, at the same time, completing their science tasks according to the goals set by their teachers. Our findings suggest that the ways in which students connect their everyday world with science learning do not have to be explicitly related to the particular conceptual learning goals but can parallel conceptual learning while contextualizing it in affectively meaningful ways.
Multi-Target Stool DNA Testing for Colorectal Cancer Screening: Emerging Learning on Real-world Performance
Authors: Eckmann Jason D. , Ebner Derek W. , Kisiel John B. 
Published: January 2020

Abstract
Purpose of Review
Multi-target stool DNA (MT-sDNA) was approved in 2014 for use in screening average-risk patients for colorectal cancer (CRC). Here, we highlight recent literature from post-market studies to provide an update on clinical use and utility not possible from pre-approval studies.

Recent Findings
MT-sDNA has been included in major society guidelines as an option for colorectal cancer screening, and has seen exponentially increasing use in clinical practice. MT-sDNA appears to be attracting new patients to CRC screening, and patient adherence to diagnostic colonoscopy after a positive MT-sDNA test is high. Approximately two-thirds of these patients are found to have colorectal neoplasia (CRN), 80% of whom have at least one right-sided lesion; 1 in 3 will have advanced CRN. High yield of CRN is due not only to post-screening increase in probability but also likely improved endoscopist attention. In those with a negative high-quality colonoscopy after positive MT-sDNA test (“false positive MT-sDNA”), further interventions do not appear to be necessary.

Summary
MT-sDNA is a promising tool to improve rates and quality of CRC screening. Further investigation should examine MT-sDNA performance in populations at increased risk for CRC, and as an interval test after colonoscopy to detect potentially missed lesions.
Positive influence of peers' interpersonal character on children's interpersonal character: The moderating role of children's and peers' social status
Authors: Liu Lisha , Xu Liangyuan , Xiao Xue , Liu Lu , Li Yanfang 
Published: January 2020

ABSTRACTIntroductionPeers are an important source of influence on children's social development. This study investigated the positive association between peers' and children's interpersonal character (i.e., humanity and justice) and the moderating role of children's and peers' social status including wealth (family SES), power (class leadership), and prestige (social preference and social visibility).MethodsThe participants were 1555 fourth‐to ninth‐graders (Mage = 12.76; 46.9% boys) and their reciprocal playmates in China. Questionnaires and peer nomination methods were used to measure interpersonal character and social status. Children's reciprocal playmates were used as the source of peer influence.ResultsThe playmates' humanity and justice were positively associated with the children's humanity and justice regardless of the child's grade, gender, or sibling status. Children's level of social visibility moderated the associations between the playmates' and the children's humanity and justice, with children of low social visibility being more strongly influenced by their playmates. The moderating role of playmates' social status was displayed in two modes and appeared in the secondary school and singleton samples. First, playmates with a higher social preference were more closely related to secondary school children's justice; second, playmates with lower social visibility were more closely related to secondary school children's and singletons' justice.ConclusionsThe findings confirm the positive relationships between peers' and children's interpersonal character and reveal an important moderating role of prestige status, especially social visibility, among the relationships. This study extends the research on positive peer influence and contributes to knowledge of peer influence mechanisms.
Top-down and bottom-up views of public choice: should wellbeing be government's only goal?
Authors: CHATER NICK 
Published: January 2020

AbstractFrijters et al. make a powerful and lucid case for a top-down approach to government, in which the maximization of wellbeing should be the ultimate goal. I argue, by contrast, for a bottom-up approach: that the variety of goals, plans, norms and rules that govern our lives should be the starting point for political discussion. From this standpoint, the goal of individual and collective decision-making of all kinds is the reconciliation of conflicting objectives and priorities on a piecemeal basis. The distinction between top-down and bottom-up approaches in political decision-making parallels debates between ‘foundationalists’ and ‘coherentists’ in epistemology.
Vitamin D-related genes and cardiometabolic markers in healthy children: a Mendelian randomisation study
Authors: Lopez-Mayorga Ania , Hauger Hanne , Petersen Rikke A. , Vogel Ulla , Damsgaard Camilla T. , Lauritzen Lotte 
Published: January 2020

AbstractObservational studies show associations between low serum 25-hydroxyvitamin D (25(OH)D) and cardiometabolic risk markers. This Mendelian randomisation study examined associations between cardiometabolic markers in children and SNP in genes related to vitamin D metabolism (DHCR7; group-specific complement (GC); cytochrome P450 subfamily IIR1 (CYP2R1); and CYP24A1) and action (CYP27B1 and VDR). In 699 healthy 8–11-year-old children, we genotyped eleven SNP. We generated a genetic risk score based on SNP associated with low 25(OH)D and investigated associations between this and blood pressure, plasma lipids and insulin. Furthermore, we examined whether SNP related to vitamin D actions modified associations between 25(OH)D and the cardiometabolic markers. All GC and CYP2R1 SNP influenced serum 25(OH)D. A risk score based on four of the six SNP was associated with 3·4 (95 % CI 2·6, 4·2) mmol/l lower 25(OH)D per risk allele (P < 0·001), but was not associated with the cardiometabolic markers. However, interactions were indicated for the three VDR SNP (Pinteraction < 0·081) on associations between 25(OH)D and TAG, systolic blood pressure and insulin, which all decreased with increasing 25(OH)D only in major allele homozygotes (β –0·02 (95 % CI –0·04, –0·01) mmol/l; β –0·5 (95 % CI –0·9, –0·1) mmHg; and β –0·5 (95 % CI –1·4, 0·3) pmol/l, respectively). In conclusion, genetic variation affected 25(OH)D substantially, but the genetic score was not associated with cardiometabolic markers in children. However, VDR polymorphisms modified associations with vitamin D, which warrants further investigation of VDR's role in the relationship between vitamin D and cardiometabolic risk.
The Kenyan Civil Forfeiture Regime: Nature, Challenges and Possible Solutions
Authors: Gikonyo Constance 
Published: January 2020

AbstractCivil forfeiture is an asset forfeiture mechanism available to seize proceeds of crime. Kenya has embraced its use and provides statutory mechanisms for its implementation. The Proceeds of Crime and Anti-Money Laundering Act is the main statute in this regard. This article examines the substantive law and procedure for civil forfeiture provided in this statute. The analysis indicates that the provisions are technical in nature and that the process is systematic. This ensures a procedurally and substantively fair process before an individual's property is seized. This approach aims to safeguard against the arbitrary deprivation of property. Nonetheless, challenges are identified that interfere with the effective implementation of the civil forfeiture regime. These problems lead to the current underutilization of the regime. Accordingly, the article identifies viable ways of addressing these shortcomings. Implementation of these suggestions could enhance the use and success of civil forfeiture in dealing with the proceeds of crime.
“Shades of Grey”: Exploring Public Opinion about Later-Life Sexuality
Authors: Gewirtz-Meydan Ateret , Ayalon Liat 
Published: January 2020

ABSTRACTPublic opinion on later-life sexuality affects older people’s sexual health, behaviour, and satisfaction. In this study, we explored public opinion on later-life sexuality by participants’ responding to an open-ended question rather than rank their level of agreement or disagreement with different statements about older people’s sexuality. Responses from 135 men and women reflecting on how sexuality changes in older age were thematically analysed. Five themes emerged, varying from positive to negative perceptions of later-life sexuality. Older people’s sexuality was described as declining, non-existent, conservative, narrow, tedious, and boring, but also as encompassing more emotions and feelings; being better and more “complete”. This study provides qualitative data and an opportunity to gain insights on valuable opinions on what young and middle-aged people think about later-life sexuality. Assessing what people think about later-life sexuality is the first stage in instilling more liberating views about the diversity of sexual expressions in old age.
HPA-axis multilocus genetic profile score moderates the association between maternal prenatal perceived stress and offspring depression in early adulthood
Authors: McKenna Brooke G. , Hammen Constance , Brennan Patricia A. 
Published: January 2020

AbstractMaternal stress during pregnancy can cause alterations to the fetal hypothalamus–pituitary–adrenal (HPA) axis, a phenomenon known as fetal programming that may have lasting effects on offspring outcomes, including depression. Evidence suggests that these effects may vary with respect to the offspring's genetic risk. Nonetheless, few studies have examined these effects into adulthood, when risk for depression onset is highest. The present study builds upon the extant literature by examining the interaction of maternal prenatal perceived stress (MPPS) and offspring HPA-axis polygenic risk to predict offspring depression in early adulthood. A total of 381 mother–child dyads participated in a prospective, longitudinal study that spanned from pregnancy until offspring were 20 years of age. Polygenic risk was defined by a multilocus genetic profile score (MGPS) that reflected the additive risk of three HPA-axis candidate genes. The results indicated that the interaction of MPPS and HPA-axis MGPS confers risk for offspring depression at age 20, in line with the differential susceptibility model. This interaction may be specific to prenatal stress, as maternal stress during early childhood did not interact with genetic risk to predict depression. These findings provide the first evidence that genetic variants that are associated with the HPA axis may act in a polygenic, additive fashion to moderate the association between fetal programming and adult depression.