bluuebunny/crossref_metadata_2025_split huggingface dataset:
Swab-Free Transport as an Optimized Preanalytical Workflow for SARS-CoV-2 Amplification
Authors: Greene Dina N , Matthys Tawna , Lockwood Christina M 
Published: October 2020

Abstract

Introduction
Efficient detection of SARS-CoV-2 will continue to be an invaluable tool for pandemic control. Current instructions specify that the collection swab should be transported within its collection media to the laboratory. Developing a process whereby this swab is removed before transport to the lab would allow for improved automation and decreased manual manipulation of samples.

Methods
A proof of principle approach was taken by eluting viral particles from flocked swabs into collection buffer with and without a mucus background. Paired swab-free and swab-containing samples were transported to the laboratory and evaluated for SARS-CoV-2 (n = 28) or RNaseP (n = 6). SARS-CoV-2 amplification was performed using the Hologic Panther Fusion Aptima and RT-PCR assays.

Results
SARS-CoV-2 was detected in all proof of principle samples with Ct values indicative of dilution. The rare exception was for a few samples where the dilution pushed the viral load below the LOD. Paired samples were 100% concordant for SARS-CoV-2 and RNaseP detection.

Conclusion
Discarding the swab after inoculating the transport buffer is an appropriate preanalytical modification. Adopting this approach can save up to 1 minute per sample. For labs processing more than 500 samples per day this equates to 1 full time equivalent shift per day.
Optimizing clinical decision support alerts in electronic medical records: a systematic review of reported strategies adopted by hospitals
Authors: Van Dort Bethany A , Zheng Wu Yi , Sundar Vivek , Baysari Melissa T 
Published: October 2020

Abstract

Objective
To identify and summarize the current internal governance processes adopted by hospitals, as reported in the literature, for selecting, optimizing, and evaluating clinical decision support (CDS) alerts in order to identify effective approaches.

Materials and methods
Databases (Medline, Embase, CINAHL, Scopus, Web of Science, IEEE Xplore Digital Library, CADTH, and WorldCat) were searched to identify relevant papers published from January 2010 to April 2020. All paper types published in English that reported governance processes for selecting and/or optimizing CDS alerts in hospitals were included.

Results
Eight papers were included in the review. Seven papers focused specifically on medication-related CDS alerts. All papers described the use of a multidisciplinary committee to optimize alerts. Other strategies included the use of clinician feedback, alert data, literature and drug references, and a visual dashboard. Six of the 8 papers reported evaluations of their CDS alert modifications following the adoption of optimization strategies, and of these, 5 reported a reduction in alert rate.

Conclusions
A multidisciplinary committee, often in combination with other approaches, was the most frequent strategy reported by hospitals to optimize their CDS alerts. Due to the limited number of published processes, variation in system changes, and evaluation results, we were unable to compare the effectiveness of different strategies, although employing multiple strategies appears to be an effective approach for reducing CDS alert numbers. We recommend hospitals report on descriptions and evaluations of governance processes to enable identification of effective strategies for optimization of CDS alerts in hospitals.
Multi-Frequency Ultrasonic Extraction of Anthocyanins from Blueberry Pomace and Evaluation of Its Antioxidant Activity
Authors: Hu Ai-Jun , Hao Shu-Ting , Zheng Jie , Chen Lin , Sun Peng-Peng 
Published: October 2020

Abstract

Background
A large amount of blueberry pomace is discarded and wasted.

Objective
To make full use of blueberry pomace and extract anthocyanins.

Method
Multi-frequency ultrasound was used to extract anthocyanins from blueberry pomace. The effects of different extraction conditions on the anthocyanin yields were studied. The antioxidant activity of anthocyanins was evaluated, and the main anthocyanin components were identified by HPLC-electrospray ionization (ESI)-MS/MS.

Results
The optimal anthocyanin extraction conditions were as follows: dual-frequency ultrasound of 40 + 80 kHz, ultrasonic power of 350 W, extraction temperature of 50°C, and extraction time of 40 min. Compared with single frequency (25 kHz) and tri-frequency ultrasound (25 + 40 + 80 kHz), the anthocyanin yield with dual-frequency ultrasound increased by 15.26% and 5.45% respectively. Furthermore, the antioxidant activities (DPPH, hydroxyl radical scavenging ability, and reducing power) of anthocyanins extracted with dual-frequency ultrasound were all higher than those without ultrasound. Seven kinds of anthocyanins were identified by HPLC-ESI-MS/MS.

Conclusions
Dual-frequency ultrasound had a higher extraction yield than single frequency ultrasound, tri-frequency ultrasound, and no ultrasound.

Highlights
Dual-frequency ultrasonic technology provides an effective approach for improving the extraction yield of anthocyanins from blueberry pomace.
Screening of Synthetic Cathinones and Metabolites in Dried Blood Spots by UPLC–MS-MS
Authors: Wang Yang , Shi Yan , Yu Yingjia , Chen Lizhu , Jiang Jiebing , Long Jiakun , Xiang Ping , Duan Gengli 
Published: October 2020

Abstract
After its use for decades in clinical screening, dried blood spots (DBS) have recently received considerable attention for their application in various novel psychoactive substances. The goal of this study was to develop and apply a DBS-based assay for 37 synthetic cathinones and their metabolites. Thirty microliters of whole blood sample after administration was spotted onto Whatman FTA classical cards, dried and extracted, and then analyzed by ultra-performance liquid chromatography–tandem mass spectrometry (UPLC–MS-MS). The samples were chromatographed on a Waters Acquity UPLC®HSS T3 column (1.8 μm, 2.1 × 100 mm) and then identically packed defender guard cartridges of a Waters Acquity UPLC®HSS T3 column (1.8 μm, 2.1 × 5 mm, 3/pk). The separation was achieved via solvents of 20 mM ammonium acetate/formic acid 0.1% (A) and acetonitrile (B) at a flow rate of 0.25 mL/min. A tandem MS equipped with positive electrospray ionization mode source was used as the detector. Multiple reaction monitoring with the precursor/product ion combinations was used to quantify each analyte. The linear range of synthetic cathinones in the DBS was 2.0–200 ng/mL, and the lowest limit of quantification was 2.0 ng/mL for some synthetic cathinones and 10 ng/mL for others. The precision and accuracy of the results for the validation samples of the synthetic cathinones were within acceptable criteria. DBS sampling offers the advantages of reduced sample volume and convenient sample storage and shipment. This method can be successfully applied to the quantification of synthetic cathinones.
To Be (a State) or Not to Be?
Authors: Heinsch Robert , Pinzauti Giulia 
Published: October 2020

Abstract
One of the traditional criteria for statehood under the Montevideo Convention is that an entity possesses an ‘effective government’. Against this backdrop, this article discusses the impact of belligerent occupation on Palestine’s ability to possess an effective government, and thus fulfil the criteria for statehood. It argues that, as a matter of law, the requirement of effective government should not be interpreted strictly. Statehood does not require an immutable threshold of effectiveness; rather, the criterion has been flexibly interpreted and applied in practice. Belligerent occupation is a factual circumstance calling for such a flexible interpretation. While occupation impairs an occupied entity’s ability to exercise the functions of government, it does not affect statehood. Within the framework of the Rome Statute, the fact that the Court has jurisdiction over situations of belligerent occupation confirms this interpretation. Moreover, when assessing whether an occupied entity meets the criteria for statehood, deficiencies in effectiveness should be weighed against the principle of self-determination and international recognition. Applying those considerations to Palestine’s situation, the conclusion seems warranted that it meets the criteria for statehood under general international law and can thus validly trigger the Court’s jurisdiction to investigate and prosecute alleged crimes committed on its territory.
Hayao Miyazaki
Authors: Greenberg Raz 
Published: October 2020

Japanese animator Hayao Miyazaki (b. 1942) is arguably the most admired figure of Japan’s postwar animation industry (commonly known as anime). Deeply moved in his youth by his country’s first color feature-length animated film Hakujaden (Panda and the Magic Serpent, 1958, directed by Taiji Yabushita), Miyazaki decided to seek a career in animation after receiving his BA degree in politics and economy. Most of his output during the first sixteen years of his work as an animator consisted of working on other directors’ films and television shows. Miyazaki made his directorial debut, sharing credit and duties with his colleague Isao Takahata, on the television series Rupan Sansei (Lupin the Third, 1971–1972), an adaptation of a popular manga (comics) series about the exploits of a daring thief. The year 1979 saw the release of Miyazaki’s feature-length debut Rupan Sansei: Kariosuturo no Shiro (Lupin the Third: The Castle of Cagliostro), a spin-off of the television series, which gained attention for its spectacular action sequences. His second feature, Kaze no Tani no Naushika (Nausicaä of the Valley of the Wind, 1984), a theatrical feature adaptation of his own long-running manga series about the quest of a pacifist princess to save a war-torn world destroyed in an environmental apocalypse, hailed for its beautiful animation, design, and environmental subtext. The success of Nausicäa of the Valley of the Wind led to the foundation of Studio Ghibli, under the creative management of Miyazaki and Takahata. A string of critically acclaimed works solidified his position as a leading director in Japan’s animation industry: the Victorian-flavored adventure Tenkū no Shiro Rapyuta (Castle in the Sky, 1986), the nostalgic children’s fantasy Tonari no Totoro (My Neighbor Totoro, 1988), the coming-of-age fantasy Majo no Takkyūbin (Kiki’s Delivery Service, 1989) and the historical comedy-adventure Kurenai no Buta (Porco Rosso, 1992). At the turn of the century, Miyazaki directed the acclaimed historical fantasy Mononoke Hime (Princess Mononoke, 1997) and the modern-day fantasy Sen to Chihiro no Kamikakushi (Spirited Away, 2001), and each became the highest-grossing film in the history of Japanese cinema, an evidence of the important position that Miyazaki has achieved in Japan’s postwar culture. Spirited Away also won the Academy Award for Best Animated Feature in 2002. Miyazaki’s later films in the 21st century met with a more mixed reception. Hauru no Ugoku Shiro (Howl’s Moving Castle, 2004), Gake no Ue no Ponyo (Ponyo, 2008), and Kaze Tachinu (The Wind Rises, 2013) were praised for their visuals, but came under criticism for their narrative qualities. The ongoing debate as to who is going to be Miyazaki’s successor as Japan’s leading animator demonstrates the deep cultural influence that his work continues to have on other animators and filmmakers.
Liu Zongzhou
Authors: Wong Simon Man Ho 
Published: October 2020

Liu Zongzhou 劉宗周 (personal name Xianzhang 憲章, courtesy name Qidong 起東, literary names Niantai 念台, Jishan 蕺山; b. 1578–d. 1645) was an important Neo-Confucian thinker in the late Ming dynasty (1368–1644) of China. Born as a posthumous child in Shanyin (Shaoxing) of the Zhejiang province, he was brought up by his mother, educated by his maternal grandfather and became a successful candidate of the metropolitan and palace examination in 1601. In 1621, as the Supplementary Secretary in the Ministry of Rites, he began to impeach the corrupt but powerful eunuch Wei Zongxian. In 1624, he declined the offer to be Junior Vice Commissioner of the Office of Transmission, and his status was reduced to that of a commoner. In 1629, he resumed office as the governor of Shuntian Prefecture, and resigned the next year to establish the Zhengren 證人 Association and to lecture at the Shigui 石匱 Academy. In 1636, he became Senior Vice Minister of Works. Yet he soon resigned to criticize the Senior Grand Secretary Wen Tiren 溫體仁, and this led to the degradation of his status to a commoner again. In 1642, he was promoted to Censor-in-chief, but he was relieved of his office when he antagonized the emperor by trying to save two censorial officials. During the fall of Beijing, he resumed his office as Censor-in-chief. He attacked the corrupt officials Ma Shiying 馬士英 and Ruan Dacheng 阮大鋮 and finally left his office. His official career lasted for forty-five years, during which he had held office six and a half years, was in active service at court only four years, and had been degraded to the status of commoner three times. With the fall of Nanjing and Hangzhou in succession to the Manchus and his decision to express his loyalty and patriotism to the country, he ended his life by fasting for twenty days. Liu distinguished himself as a Neo-Confucian philosopher and scholar. The main doctrines of his teaching are “vigilance in solitude” (shendu慎獨) and “sincerity of will” (chengyi誠意), which originate from the two Confucian classics Doctrine of the Mean and Great Learning. Huang Zongxi 黃宗羲 (b. 1610–d. 1695), his important disciple and a well-known intellectual historian, placed him and his school of thought in the last part of Huang’s influential work, The Records of Ming Scholars. Huang not only compared him to the most significant Neo-Confucian philosophers, but also hinted that his philosophy signified the final summation of the Neo-Confucian tradition from the Song to Ming dynasties. He is commonly regarded as one of the most important Song-Ming Neo-Confucian thinkers. It is the creativity and depth of his philosophy that deserves scholars’ attention.
Luca della Robbia (or the Della Robbia Family)
Authors: Kupiec Catherine 
Published: October 2020

Florentine sculptor Luca della Robbia (b. 1399/1400–d. 1482) is best remembered as the inventor of a popular new form of glazed terracotta sculpture and as founder of the flourishing family workshop that produced it for roughly a century. He has long ranked among the greatest artists of the early Florentine Renaissance: tellingly, Leon Battista Alberti celebrated him as one of five exceptional modern artists in the dedication to his 1436 treatise On painting (Della pittura), alongside Filippo Brunelleschi, Donatello, Lorenzo Ghiberti, and Masaccio. Luca was a consummate craftsman, praised by writers during and after his lifetime for his activity in marble, bronze, terracotta, and even goldsmithery. Modern scholarship on the artist is substantial and has consistently sounded two notes, celebrating his exemplary classicizing style and recognizing that the story of his artistic success is also, simultaneously, that of a new sculptural medium. While the exact details of its initial development (accomplished by the year 1441) remain unknown, Luca’s glazed terracotta art soon attracted eminent patrons, such as Piero de’ Medici, and was praised by contemporaries as an invention. In the tradition of artistic family dynasties, Luca passed the secrets of his new art to his nephew, Andrea della Robbia (b. 1435–d. 1525), who succeeded him as head of the workshop. Andrea conducted a brisk business in glazed sculpture and taught the family craft to five of his sons: Marco (Fra Mattia, b. 1468–d. 1534), Giovanni (b. 1469–d. 1529/1530), Luca “il giovane” (b. 1475–d. 1548), Francesco (Fra Ambrogio, b. 1477–d. 1527/1528), and Girolamo (b. 1488–d. 1566). Andrea and Giovanni are the best-studied among these artists, while the others long suffered from general neglect owing to their geographically disperse activity and a perception that production quality fell with the later generations. Similarly under-studied are two final artists who made glazed sculptures: the Florentine Benedetto Buglioni (b. 1459/1460–d. 1521), who likely trained under Andrea and opened his own shop around 1480, and his adopted ward, Santi Buglioni (b. 1494–d. 1576). Recent scholarship has illuminated the ongoing efforts of the later artists in both families to integrate workshop traditions forged under Luca with new contexts and artistic innovations, all while serving patrons ranging from Franciscan friars to King François I of France.
A self-report questionnaire to detect hand dermatitis in nurses
Authors: Parsons V , Williams H C , English J , Llewellyn J , Ntani G , Madan I 
Published: October 2020

Abstract

Background
Hand dermatitis is highly prevalent among nurses due to their frequent exposure to wet work. Providing cost-effective dermatological health surveillance for this occupational group presents a challenge to health service providers.

Aims
To ascertain the predictive value of nurses’ self-assessment of whether they had current hand dermatitis using a screening questionnaire when compared with the assessment made by a dermatologist of the nurses’ hand photographs.

Methods
We conducted a cross-sectional study comparing the self-report decision made by student and intensive care nurses using a single hand dermatitis screening question with the clinical assessment of their hand photographs made by dermatologists using a standardized photographic guide.

Results
We analysed data collected at study baseline (n = 1599). The results showed that the screening question had a high negative predictive value (91%; 95% CI 89–93), but a low positive predictive value (39%; 95% CI 34–45). It demonstrated acceptable accuracy in distinguishing those with and without the disease (area under the receiver operator curve = 0.7) and had a high specificity (86%; 95% CI 84–88) but a sensitivity of only 52% (95% CI 46–59) in identifying hand dermatitis.

Conclusions
We found that nurses were able to accurately self-assess themselves as not having any signs of hand dermatitis. By contrast, they were less able to accurately self-assess positive cases suggesting under-recognition of early disease. We propose that a questionnaire containing a single hand dermatitis screening question should be considered as a tool for screening out clear cases as part of a workplace health surveillance programme for detecting hand dermatitis.
A survey of current levels of trace minerals and vitamins used in commercial diets by the Brazilian pork industry—a comparative study
Authors: Dalto Danyel Bueno , da Silva Caio Abércio 
Published: October 2020

AbstractThis study identified the levels of trace minerals and vitamins used in commercial diets by the Brazilian pork industry and compared these levels against the Brazilian reference tables and those from the North American pork industry. Animal feed companies (n = 15) and cooperatives/agro-industries (n = 15) from the Brazilian pork sector participated in this study. Levels of vitamin A, D, E, and K, thiamine, riboflavin, niacin, pantothenic acid, pyridoxine, biotin, folic acid, vitamin B12, choline, vitamin C, cobalt, copper, chromium, iodine, iron, manganese, selenium, and zinc were collected. Data were compiled by feeding phase to determine descriptive statistics. Ratios were calculated comparing the observed levels to those from the Brazilian reference tables and those from the North American pork industry. Average levels of trace minerals and vitamins used by the Brazilian pork industry were between 40% and 240% higher than the recommendations of the Brazilian reference tables. Compared to the North American pork industry, Brazilian levels for trace minerals were overall higher but for water-soluble vitamins they were significantly lower. Large between-companies variations were observed for most of the studied nutrients, especially for water-soluble vitamins. In conclusion, the Brazilian pork industry adds a significant margin of safety for trace minerals and vitamins supplementation. The large between-companies variations, especially for water-soluble vitamins, reflect the limited knowledge available on precise levels of supplementation for trace nutrients for pigs.
Visual Field Artifacts in Glaucoma With Face Mask Use During the COVID-19 Pandemic
Authors: El-Nimri Nevin W. , Moghimi Sasan , Fingeret Murray , Weinreb Robert N. 
Published: October 2020

Purpose:
The coronavirus (COVID-19) pandemic has changed how outpatient care is delivered in ophthalmology clinics, particularly with glaucoma care. This case series highlights the need for awareness of fogging and improper face mask fit as causes of standard automated perimetry artifacts in patients with ocular hypertension and glaucoma.

Clinical Presentations:
Six patients with the diagnosis of ocular hypertension, glaucoma suspect, or glaucoma underwent standard automated perimetry (24-2 or 10-2 SITA, Humphrey Field Analyzer) while wearing ear-loop surgical face masks. Due to patient complaints of fogging during the testing, low test reliability, and unexpected results, the tests were repeated after taping securely the mask to the bridge of the nose.

Clinical Findings:
Fogging may reduce visual field (VF) test reliability and induce artifacts that mimic glaucomatous defects. VF test reliability can be improved and artifacts minimized following mask taping. In 1 case there was worsening of VF defects after mask taping. This suggests that fogging may also disguise true VF defects.

Conclusions:
Fogging can result in unreliable VF testing with glaucoma-like artifacts. Secure taping of the face mask to the nose bridge may minimize this problem and reduce unnecessary additional testing and follow-up visits.