bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association between indicators of systemic inflammation biomarkers during puberty with breast density and onset of menarche
Authors: Michels Karin B. , Keller Kristen , Pereira Ana , Kim Claire E. , Santos José L. , Shepherd John , Corvalan Camila , Binder Alexandra M. 
Published: October 2020

Abstract
Background
Systemic inflammation may play a role in shaping breast composition, one of the strongest risk factors for breast cancer. Pubertal development presents a critical window of breast tissue susceptibility to exogenous and endogenous factors, including pro-inflammatory markers. However, little is known about the role of systemic inflammation on adolescent breast composition and pubertal development among girls.

Methods
We investigated associations between circulating levels of inflammatory markers (e.g., interleukin-6 (IL-6), tumor necrosis factor receptor 2 (TNFR2), and C-reactive protein (CRP)) at Tanner stages 2 and 4 and breast composition at Tanner stage 4 in a cohort of 397 adolescent girls in Santiago, Chile (Growth and Obesity Cohort Study, 2006–2018). Multivariable linear models were used to examine the association between breast composition and each inflammatory marker, stratifying by Tanner stage at inflammatory marker measurement. Accelerated failure time models were used to evaluate the association between inflammatory markers concentrations at each Tanner stage and time to menarche.

Results
In age-adjusted linear regression models, a doubling of TNFR2 at Tanner 2 was associated with a 26% (95% CI 7–48%) increase in total breast volume at Tanner 4 and a 22% (95% CI 10–32%) decrease of fibroglandular volume at Tanner 4. In multivariable models further adjusted for body fatness and other covariates, these associations were attenuated to the null. The time to menarche was 3% (95% CI 1–5%) shorter among those in the highest quartile of IL-6 at Tanner 2 relative to those in the lowest quartile in fully adjusted models. Compared to those in the lowest quartile of CRP at Tanner 4, those in the highest quartile experienced 2% (95% CI 0–3%) longer time to menarche in multivariable models.

Conclusions
Systemic inflammation during puberty was not associated with breast volume or breast density at the conclusion of breast development among pubertal girls after adjusting for body fatness; however, these circulating inflammation biomarkers, specifically CRP and IL-6, may affect the timing of menarche onset.
Rapid detection of Galba truncatula in water sources on pasture-land using loop-mediated isothermal amplification for control of trematode infections
Authors: Davis Chelsea N. , Tyson Fiona , Cutress David , Davies Emma , Jones Dewi Llyr , Brophy Peter M. , Prescott Alex , Rose Michael T. , Williams Manod , Williams Hefin Wyn , Jones Rhys Aled 
Published: September 2020

Abstract
Background
Fascioliasis caused by the trematodes Fasciola hepatica and F. gigantica, is a global neglected zoonotic disease estimated to cost the livestock industry over €2.5 billion annually. Farm management measures and sustainable use of anthelmintics can, in principle, effectively control trematode infection in livestock and reduce the rate of developing anthelmintic resistance. Previously, we designed an environmental DNA (eDNA) assay to identify a common trematode intermediate host, the freshwater snail Galba truncatula, in water sources to measure specific trematode infection risk areas on pasture-land. To improve this procedure, we now report a loop-mediated isothermal amplification (LAMP) assay to identify G. truncatula eDNA.

Methods
A LAMP assay was designed and optimised (e.g. temperature, time duration and primer concentration) to identify G. truncatula DNA. The ability of the LAMP assay to target G. truncatula DNA was identified, and LAMP assay limit of detection was investigated in comparison to conventional PCR. In the field, 48 water samples were collected from stream, ditch and water pool habitats in four locations at two Aberystwyth University farms over a seven week period to investigate the applicability of the LAMP assay for use on eDNA samples, in comparison to conventional PCR.

Results
The LAMP assay delivered detectable results in 30 min at 63 °C. The assay discriminated between G. truncatula DNA and non-target DNA, presenting a level of DNA detection comparable to conventional PCR. No significant difference was found between the ability of the LAMP and PCR assay to identify G. truncatula eDNA in water samples. Kappa coefficient analysis revealed a moderate level of agreement between LAMP and PCR assays.

Conclusions
This study demonstrated that the LAMP assay can detect G. truncatula eDNA in a simple and rapid manner. The LAMP assay may become a valuable tool to determine optimum pasture management for trematode parasite control.
Tabanids as possible pathogen vectors in Senegal (West Africa)
Authors: Keita Mohamed Lamine , Medkour Hacène , Sambou Masse , Dahmana Handi , Mediannikov Oleg 
Published: October 2020

Abstract
Background
Species of the Tabanidae are potent vectors of human and animal diseases, but they have not been thoroughly investigated to date. In Senegal (West Africa), little information is available on these dipterans. Our objective in this study was to investigate Senegalese tabanids and their diversity by using molecular and proteomics approaches, as well as their associated pathogens.

Methods
A total of 171 female tabanids were collected, including 143 from Casamance and 28 from Niokolo-Koba. The samples were identified morphologically by PCR sequencing and by MALDI-TOF MS, and PCR analysis was employed for pathogen detection and blood-meal characterization.

Results
The morphological identification revealed four species concordantly with the molecular identification: Atylotus fuscipes (79.5%), Tabanus guineensis (16.4%), Chrysops distinctipennis (3.5%) and Tabanus taeniola (0.6%) (not identified by PCR). The molecular investigation of pathogens revealed the presence of Trypanosoma theileri (6.6%), Leishmania donovani (6.6%), Setaria digitata (1.5%), Rickettsia spp. (5.1%) and Anaplasmataceae bacteria (0.7%) in A. fuscipes. Tabanus guineensis was positive for L. donovani (35.7%), S. digitata (3.6%) and Anaplasmataceae (17.8%). Leishmania donovani has been detected in 50% of C. distinctipennis specimens and the only T. taeniola specimen. No Piroplasmida, Mansonella spp. or Coxeilla burnetii DNA was detected. In addition to humans (96.43%), Chlorocebus sabeus, a non-human primate, has been identified as a host of (3.57%) analysed tabanids. MALDI-TOF MS enabled us to correctly identify all tabanid species that had good quality spectra and to create a database for future identification.

Conclusions
Tabanids in Senegal could be vectors of several pathogens threatening animal and public health. To fully characterize these dipterans, it is therefore necessary that researchers in entomology and infectiology employ molecular characterization and mass spectrometric techniques such as MALDI-TOF MS to analyse these dipterans in Senegal and West Africa.
Existence results for first derivative dependent ϕ-Laplacian boundary value problems
Authors: Talib Imran , Abdeljawad Thabet 
Published: October 2020

AbstractOur main concern in this article is to investigate the existence of solution for the boundary-value problem$$\begin{aligned}& (\phi \bigl(x'(t)\bigr)'=g_{1} \bigl(t,x(t),x'(t)\bigr),\quad \forall t\in [0,1], \\& \Upsilon _{1}\bigl(x(0),x(1),x'(0)\bigr)=0, \\& \Upsilon _{2}\bigl(x(0),x(1),x'(1)\bigr)=0, \end{aligned}$$(ϕ(x′(t))′=g1(t,x(t),x′(t)),∀t∈[0,1],ϒ1(x(0),x(1),x′(0))=0,ϒ2(x(0),x(1),x′(1))=0,where$g_{1}:[0,1]\times \mathbb{R}^{2}\rightarrow \mathbb{R}$g1:[0,1]×R2→Ris an$L^{1}$L1-Carathéodory function,$\Upsilon _{i}:\mathbb{R}^{3}\rightarrow \mathbb{R} $ϒi:R3→Rare continuous functions,$i=1,2$i=1,2, and$\phi :(-a,a)\rightarrow \mathbb{R}$ϕ:(−a,a)→Ris an increasing homeomorphism such that$\phi (0)=0$ϕ(0)=0, for$0< a< \infty $0<a<∞. We obtain the solvability results by imposing some new conditions on the boundary functions. The new conditions allow us to ensure the existence of at least one solution in the sector defined by well ordered functions. These ordered functions do not require one to check the definitions of lower and upper solutions. Moreover, the monotonicity assumptions on the arguments of boundary functions are not required in our case. An application is considered to ensure the applicability of our results.
A role for the alpha-8 integrin chain (itga8) in glomerular homeostasis of the kidney
Authors: Marek Ines , Hilgers Karl Friedrich , Rascher Wolfgang , Woelfle Joachim , Hartner Andrea 
Published: September 2020

AbstractGlomerulonephritis results in a dysregulation of glomerular cells and may end up in chronic alterations and subsequent loss of renal function. Therefore, understanding mechanisms, which contribute to maintain glomerular integrity, is a pivotal prerequisite for therapeutic interventions. The alpha-8 integrin chain seems to be an important player to maintain glomerular homeostasis by conferring mechanical stability and functional support for the renal capillary tuft.
Back protection of canvas paintings
Authors: Padfield Tim , Padfield Nicolas , Lee Daniel Sang-Hoon , Thøgersen Anne , Nielsen Astrid Valbjørn , Andersen Cecil Krarup , Scharff Mikkel 
Published: October 2020

Abstract
In this paper different scenarios for back protection of a canvas painting and their effect on the stability of the relative humidity behind the painting are tested. A painting on canvas, stretched on a wooden frame, was fitted with various styles of back protection and then exposed to a cycle of temperature variation at the back, with the front exposed to a constant room temperature. The painting was also exposed to a constant wall temperature and varying room temperature. The space between the canvas and the back board was fitted with temperature and relative humidity (RH) sensors. The sensors were used to provide the essential single-point data of temperature and RH at the given locations. For more comprehensive understanding of the rather confined space, further numerical simulation (computational fluid dynamics) was adopted as part of the investigation. The computational fluid dynamics was used to understand the natural convection within the microclimate through the depictions of temperature distribution, as well as the corresponding airflow. The unprotected painting suffered a large RH variation at its back, because of the varying canvas temperature interacting with the constant room air moisture content. Effective stabilisation of the RH behind the canvas against temperature variation was provided by a shiny aluminium alloy sheet sealed against the frame. The non-absorbent back board experienced a strong variation in RH, because of humidity buffering of the space by the painting canvas at a different temperature. Either a space or insulation between this back plate and the wall reduced the risk of condensation on the inner surface of the back plate. Insulation will however increase the risk of condensation on the wall surface behind the painting. An absorbent back board de-stabilised the RH at the painting canvas surface by providing a competing humidity buffer at a different temperature. To provide protection against moisture exchange with an unsuitable room RH, extra humidity buffer was placed 3 mm behind the painting canvas, kept close to the painting temperature by insulation between this buffer and the back board. This stabilised RH at the canvas surface but increased both the temperature and the RH variation at the back board and thus increased the risk of condensation on the inner surface of the back board. The RH and the temperature in the narrow spaces between the painting canvas and the wooden stretcher frame were always more nearly constant than in the open canvas area, which suggests an explanation for the widely observed better condition of the areas of canvas paintings which lie close over the support structure. Our conclusion is that a non-absorbent, impermeable back plate gives good RH stability against a changing temperature gradient between wall and canvas painting surface.
Sandbox security model for Hadoop file system
Authors: Gousiya Begum  , Huq S. Zahoor Ul , Kumar A. P. Siva 
Published: September 2020

AbstractExtensive usage of Internet based applications in day to day life has led to generation of huge amounts of data every minute. Apart from humans, data is generated by machines like sensors, satellite, CCTV etc. This huge collection of heterogeneous data is often referred as Big Data which can be processed to draw useful insights. Apache Hadoop has emerged has widely used open source software framework for Big Data Processing and it is a cluster of cooperative computers enabling distributed parallel processing. Hadoop Distributed File System is used to store data blocks replicated and spanned across different nodes. HDFS uses an AES based cryptographic techniques at block level which is transparent and end to end in nature. However cryptography provides security from unauthorized access to the data blocks, but a legitimate user can still harm the data. One such example was execution of malicious map reduce jar files by legitimate user which can harm the data in the HDFS. We developed a mechanism where every map reduce jar will be tested by our sandbox security to ensure the jar is not malicious and suspicious jar files are not allowed to process the data in the HDFS. This feature is not present in the existing Apache Hadoop framework and our work is made available in github for consideration and inclusion in the future versions of Apache Hadoop.
Comparison of the different voxel sizes in the estimation of peri-implant fenestration defects using cone beam computed tomography: an ex vivo study
Authors: Kurt Mehmet Hakan , Bağış Nilsun , Evli Cengiz , Atakan Cemal , Orhan Kaan 
Published: October 2020

Abstract
Background
To examine the influence of voxel sizes to detect of peri-implant fenestration defects on cone beam computed tomography (CBCT) images.

Materials and methods
This study performed with three sheep heads both maxilla and mandible and two types of dental implant type 1 zirconium implant (Zr40) (n = 6) and type 2 titanium implant (Ti22) (n = 10). A total of 14 peri-implant fenestrations (8 buccal surfaces, 6 palatal/lingual surface) were created while 18 surfaces (8 buccal, 10 palatal/lingual) were free of fenestrations. Three observers have evaluated the images of fenestration at each site. Images obtained with 0.75 mm3, 0.100 mm3, 0.150 mm3, 0.200 mm3, and 0.400 mm3 voxel sizes. For intra- and inter-observer agreements for each voxel size, Kappa coefficients were calculated.

Results
Intra- and inter-observer kappa values were the highest for 0.150 mm3, and the lowest in 0.75 mm3 and 0.400 mm3 voxel sizes for all types of implants. The highest area under the curve (AUC) values were found higher for the scan mode of 0.150 mm3, whereas lower AUC values were found for the voxel size for 0.400 mm3. Titanium implants had higher AUC values than zirconium with the statistical significance for all voxel sizes (p ≤ 0.05).

Conclusion
A voxel size of 0.150 mm3 can be used to detect peri-implant fenestration bone defects. CBCT is the most reliable diagnostic tool for peri-implant fenestration bone defects.
Minimally invasive surgical repair for unroofed coronary sinus syndrome directed by three-dimensional transesophageal echocardiography
Authors: Handa Kazuma , Fukui Shinya , Kitahara Mutsunori , Kakizawa Yumi , Nishi Hiroyuki 
Published: October 2020

Abstract
Background
The recent remarkable development of cardiac imaging technology for unroofed coronary sinus syndrome has led to accurate preoperative diagnosis. We report a case of unroofed coronary sinus syndrome repaired via a minimally invasive approach, under the excellent command of three-dimensional transesophageal echocardiography.

Case presentation
A 77-year-old woman with hypertension was admitted for aggravation of bilateral leg edema and diagnosed with type III unroofed coronary sinus syndrome with Qp/Qs ratio of 1.6:1. The unroofed portion was detected at the atrial side between P2 and P3 of posterior mitral leaflet by preoperative three-dimensional transesophageal echocardiography. Right minithoracotomy was performed at the fourth intercostal space and cardiopulmonary bypass routinely established. Right atriotomy and left atriotomy incisions were made under antegrade cardioplegic arrest. The unroofed portion was revealed at the same location by preoperative transesophageal echocardiography and was clearly recognized only by endoscopy, not by direct vision. It was repaired by direct running suture under endoscopic visualization. We observed no blood cardioplegia leakage or mitral insufficiency, which was also confirmed by postoperative transesophageal echocardiography. The patient’s postoperative course was uneventful and she was discharged home 14 days after surgery without any residual shunt.

Conclusions
Successful repair of unroofed coronary sinus syndrome was safely and effectively achieved by a minimally invasive approach supported by preoperative three-dimensional transesophageal echocardiography.
Use of anterolateral thigh flap for reconstruction of traumatic bilateral hemipelvectomy after major pelvic trauma: a case report
Authors: Al-wageeh Saleh , Ahmed Faisal , Al-naggar Khalil , Askarpour Mohammad Reza , Al-shami Ebrahim 
Published: October 2020

Abstract
Background
Major pelvic trauma (MPT) with traumatic hemipelvectomy (THP) is rare, but it is a catastrophic health problem caused by high-energy injury leading to separation of the lower extremity from the axial skeleton, which is associated with a high incidence of intra-abdominal and multi-systemic injuries. THP is generally performed as a lifesaving protocol to return the patient to an active life.

Case report
A 12-year male patient exposed to major pelvic trauma with bilateral THP survived the trauma and multiple lifesaving operations. The anterolateral thigh flap is the method used for wound reconstruction. The follow-up was ended with colostomy and cystostomy with wheelchair mobilization. To the best of our knowledge, there have been a few bilateral THP reports, and our case is the second one to be successfully treated with an anterolateral thigh flap.

Conclusion
MPT with THP is the primary cause of death among trauma patients. Life-threatening hemorrhage is the usual cause of death, which is a strong indication for THP to save life.
Ulcerative colitis that developed during radiotherapy for prostate cancer, deteriorated rapidly and required emergency surgery
Authors: Matsuda Keiji , Okada Yuka , Hashiguchi Yojiro , Asako Kentaro , Ohno Kohei , Tsukamoto Mitsuo , Fukushima Yoshihisa , Shimada Ryu , Ozawa Tsuyoshi , Hayama Tamuro , Nozawa Keijiro , Fukagawa Takeo , Sasajima Yuko 
Published: October 2020

Abstract
Background
Although there are reports linking ulcerative colitis (UC) to prostate cancer (PC), those reports are of PC patients who were previously diagnosed with UC. There are no reports of the development of UC during radiotherapy. Here we describe the first case of a patient who developed UC during radiotherapy for PC. The UC progressed rapidly and required emergency surgery.

Case presentation
A 61-year-old Japanese man underwent a prostate biopsy at another hospital due to a high prostate-specific antigen level and was diagnosed with PC. Goserelin and bicalutamide treatment was initiated in 2019, and intensity-modulated radiotherapy (total of 60 Gy/20 Fr) was administered in 2020. Diarrhea began during the radiotherapy and bleeding began post-radiotherapy. He was admitted to another hospital 14 days after the end of the radiotherapy, and colonoscopy revealed a deep ulcer in the colon, which led to the suspicion of UC. He was transferred to our hospital, and colonoscopy showed a widespread map-like ulcer, pseudopolyposis, and very easy bleeding in the colon. We diagnosed severe UC, and it worsened rapidly with uncontrollable bleeding, which we considered an indication for surgery. Emergency surgery (a total colectomy and ileostomy creation) was performed. The specimens confirmed an extensively spreading ulcer throughout the colon. The pathological report was UC in the active phase. The postoperative course was good.

Conclusions
When a patient exhibits diarrhea while undergoing radiotherapy for PC, clinicians should be aware of the possibility of UC in addition to radiation colitis, and colonoscopy should be considered.
Intestinal anisakiasis with severe intestinal ischemia caused by extraluminal live larvae: a case report
Authors: Shibata Kengo , Yoshida Yuichi , Miyaoka Yoichi , Emoto Shin , Kawai Tomoaki , Kobayashi Seiji , Ogasawara Kazuhiro , Taketomi Akinobu 
Published: October 2020

Abstract
Background
Anisakiasis is a parasitic infection caused by Anisakis worms found in raw fish. Most cases of anisakiasis occur in the stomach and rarely occur in the intestine. It is extremely rare for live larvae to break through the intestine into the mesentery and cause severe intestinal ischemia. Anisakiasis can be treated conservatively, because the larvae will die in approximately 1 week, but, sometimes, a serious condition can arise, as in this case. We report the first case of extraluminal anisakiasis in which a live Anisakis worm caused severe intestinal ischemia.

Case presentation
The patient was a 26-year-old woman who ate squid a week prior. She had abdominal pain and was admitted to our emergency department. On physical examination, abdominal guarding and rebound tenderness were present in her lower abdomen. Contrast-enhanced computed tomography showed ascites, the whirl sign, localized submucosal edema of the intestinal wall, and a dilated small bowel segment with edema. We suspected the strangulated small bowel obstruction based on the CT-scan findings. To rule out the strangulated small bowel obstruction, laparoscopic exploration was performed. Bloody ascites in the pouch of Douglas and severe inflammation in 20 cm of the ileum were observed. An Anisakis larva had perforated the intestinal wall and was found alive in the mesentery. The ileum had developed a high degree of ischemia, so the affected section was resected. Histopathological examination revealed that the Anisakis worm body was in the inflamed mesentery and caused a high degree of ischemia in the intestinal tract. The patient was discharged 9 days after surgery.

Conclusions
A living Anisakis larva punctured the mesentery of the small intestine, resulting in severe intestinal ischemia. As seen in this case, intestinal anisakiasis may cause serious symptoms, and a low threshold for performing diagnostic laparoscopy for the early diagnosis of bowel ischemia secondary to anisakiasis can be useful in determining the definite diagnosis and indications for resection.
Current knowledge of large vascular occlusion due to intracranial atherosclerosis: focusing on early diagnosis
Authors: Li He , Liu Peng , Liu Pei , Hua Weilong , Yang Wenjin , Zhang Yongxin , Zhang Lei , Xing Pengfei , Li Zifu , Zhang Yongwei , Hong Bo , Yang Pengfei , Liu Jianmin 
Published: October 2020

AbstractIntracranial atherosclerosis (ICAS)-related large vascular occlusion (LVO) was an intractable subtype of acute ischemic stroke (AIS), which always needed rescue angioplasty and stenting and complicated the procedure of endovascular recanalization. Diagnosing ICAS-LVO accurately and early was helpful for both clinical treatment and trials. Digital subtraction angiography (DSA) was unable to provide an early and rapid diagnosis of ICAS-LVO based on current studies. A variety of pre-DSA methods had been used to distinguish ICAS-LVO with other subtypes of ischemic stroke, such as medical histories, clinical presentations, computed tomography or angiography (CT/CTA), and magnetic resonance imaging (MRI/MRA). This article briefly reviewed the status quo of the diagnosis and treatment of ICAS-LVO and summarized early diagnostic methods of ICAS-LVO from different aspects.