bluuebunny/crossref_metadata_2025_split huggingface dataset:
Synthesis of a novel 89Zr labeled HER2 affibody and its application study in tumor PET imaging
Authors: Xu Yuping , Wang Lizhen , Pan Donghui , Yan Junjie , Wang Xinyu , Yang Runlin , Li Mingzhu , Liu Yu , Yang Min 
Published: June 2020

Abstract
Background: Human epidermal growth factor receptor-2 (HER2) is an essential biomarker for tumor treatment. Affibody is an ideal vector for preparing HER2 specific probes because of high affinity and rapid clearance from normal tissues etc. Zirconium-89 is a PET imaging isotope with a long half-life and suitable for monitoring biological processes for more extended periods. In this study, a novel 89Zr-labeled HER2 affibody, [89Zr]Zr-DFO-MAL-Cys-MZHER2, was synthesized, and its imaging characters were also assessed. Results: The precursor, DFO-MAL-Cys-MZHER2, was obtained with a yield of nearly 50%. The radiochemical yield of [89Zr]Zr -DFO-MAL-Cys-MZHER2 was 90.2±1.9% , and the radiochemical purity was higher than 95%. The total synthesis time was only 30 minutes. The probe was stable in PBS and serum. The tracer accumulated in HER2 overexpressing human ovarian cancer SKOV-3 cells. In vivo studies in mice bearing tumors showed that the probe highly retained in SKOV-3 xenografts even for 48 hours. The tumors were visualized with good contrast to normal tissues. ROI analysis revealed that the average uptake values in the tumor were greater than 5%IA/g during 48 hours postinjection. On the contrary, the counterparts of MCF-7 tumors kept low levels（~1%IA/g）. The outcome was consistent with the immunohistochemical analysis and ex vivo autoradiography. The probe quickly cleared from the normal organs except kidneys and mainly excreted through the urinary system. Conclusion: The novel HER2 affibody for PET imaging was easily prepared with satisfactory labeling yield and radiochemical purity. [89Zr]Zr-DFO-MAL-Cys-MZHER2 is a potential candidate for detecting HER2 expression. It may play specific roles in clinical cancer theranostics.
WITHDRAWN: The mechanism of jian-Gan-Xiao-Zhi decoction in IR adipocytes and its component analysis
Authors: Fang Fang , Wen WeiBo , Xie Xuehua , Yang Ling , Zhang Xu , Zhao jie 
Published: June 2020

Abstract
Background: Jian-Gan-Xiao-Zhi decoction (JGXZ) is a formula of traditional Chinese medicine to treat patients with NAFLD. Thus, the study aimed to analyze the mechanism of drug serum of JGXZ in adipocytes and detect the main components of JGXZ in rat serum. Methods: The 3T3-L1 preadipocytes were used to establish IR adipocytes model. The lipid accumulation in adipocytes was detected by oil red O staining. After JGXZ treatment, glucose consumption, TC and TG were analyzed by corresponding kits. ROS levels were measured by flow cytometry. In addition, Western Blot was used to assess LKB1/AMPK and JNK/IRS/PI3k/AKT expressions. Except for that, the main components of JGXZ in rat serum samples were detected by LC-MS/MS method. A Phenomenex Luna C18 was used to perform chromatographic separation and detect the concentration of main components in JGXZ. The ion source was ESI. The mobile phase contained methanol and 0.1% formic acid solution. Results: JGXZ significantly decreased glucose levels and adipogenesis, accompanied by decreased IR (p<0.01). Besides, JGXZ markedly affected ROS, LKB1/AMPK and JNK/IRS/PI3k/AKT levels(p<0.01). The R1, Rg1, paeoniflorin, Rb1, astragaloside and tanshinone could be significantly quantified. Conclusions: Thus, JGXZ decreased glucose and lipid synthesis possibly via ROS/AMPK/JNK pathway. R1, Rg1, paeoniflorin, Rb1, astragaloside and tanshinone in JGXZ could play main roles in treating NAFLD, which could offer assistance for us to study the mechanism of JGXZ on treating NAFLD.
Cross-Contamination by Disinfectant Towelettes Varies by Product Chemistry and Strain
Authors: Voorn Maxwell , Goss Summer , Nkemngong Carine , Li Xiaobao , Teska Peter , Oliver Haley F 
Published: June 2020

Abstract
Background: Disinfectant products are used frequently on environmental surfaces (e.g. medical equipment, countertops, patient beds) and patient care equipment within healthcare facilities. The purpose of this study was to assess the risk of cross-contamination of Staphylococcus aureus and Pseudomonas aeruginosa during and after disinfection of predetermined surface areas with ready-to-use (RTU) pre-wetted disinfectant towelettes. Methods: This study tested six disinfectant towelette products against S. aureus ATCC CRM-6538 and P. aeruginosa strain ATCC-15442 on Formica surfaces. Each disinfectant was evaluated on a hard nonporous surface and efficacy was measured every 0.5 m2 using a modified version of EPA MLB SOP-MB-33 to study the risk of cross-contamination. Results: We found that all of the wipes used in this study transferred P. aeruginosa and S. aureus from an inoculated surface to previously uncontaminated surfaces. Disinfectant towelettes with certain chemistries also retained a high level of viable bacteria after disinfection of the surface area. The cross-contamination risk also varied by product chemistry and bacterial strain. Conclusion: Disinfectant wipes can cross-contaminate hard nonporous surfaces and retain viable bacterial cells post-disinfection, especially over larger surface areas. This highlights a need to further investigate the risk disinfectant wipes pose during and post-disinfection and guidance on maximum surface areas treated with a single towelette.
The oncogenic potential of a mutant TP53 gene explored in two spontaneous lung cancer mice models
Authors: Ramelow Julian , Brooks Christopher , GaO Li , Almiman Abeer A , Williams Terence M , Villalona-Calero Miguel A , duan wenrui 
Published: June 2020

Abstract
Background: Lung cancer is the number one cancer killer worldwide. A major impediment to progress in the lung cancer treatment field is the lack of realistic mouse models that replicate the complexity of human malignancy and immune contexture within the tumor microenvironment. Such models are urgently needed. Mutations of the tumor suppressor gene TP53 are among the most common alterations in human lung cancers. Methods: Previously, we developed a line of lung cancer mouse model where mutant human TP53-273H is expressed in a lung specific manner in FVB/N background. To investigate whether the human TP53 mutant has a similar oncogenic potential when it is expressed in another strain of mouse, we crossed the FVB/N-SPC-TP53-273H mice to A/J strain and created A/J-SPC-TP53-273H transgenic mice. We then compared lung tumor formation between A/J-SPC-TP53-273H and FVB/N-SPC-TP53-273H. Results: We found the TP53-273H mutant gene has a similar oncogenic potential in lung tumor formation in both mice strains, although A/J strain mice have been found to be a highly susceptible strain in terms of carcinogen-induced lung cancer. Both transgenic lines survived more than 18 months and developed age related lung adenocarcinomas. With micro CT imaging, we found the FVB-SPC-TP53-273H mice survived more than 8 weeks after initial detection of lung cancer, providing a sufficient window for evaluating new anti-cancer agents. Conclusions: Oncogenic potential of the most common genetic mutation, TP53-273H, in human lung cancer is unique when it is expressed in different strains of mice. Our mouse models are useful tools for testing novel immune check point inhibitors or other therapeutic strategies in treatment of lung cancer.
Aortic stiffness and central hemodynamics in treatment naïve HIV infection: A cross-sectional study.
Authors: Martínez-Ayala Pedro , Alanis-Sánchez Guillermo Adrian , Gonzalez-Hernández Luz Alicia , Álvarez-Zavala Monserrat , Cabrera-Silva Rodolfo Ismael , Andrade-Villanueva Jaime Federico , Sánchez-Reyes Karina , Ramos-Solano Moisés , Castañeda-Zaragoza Diego Alberto , Cardona-Müller David , Tostuka-Sutto Sylvia , Cardona-Muñoz Ernesto , Ramos-Becerra Carlos Gerardo 
Published: June 2020

Abstract
Background: Human immunodeficiency virus (HIV) infection is associated with a greater risk of cardiovascular disease (CVD). HIV infection causes a chronic inflammatory state and increases oxidative stress which can cause endothelial dysfunction and arterial stiffness. Aortic stiffness measured by carotid femoral-pulse wave velocity (cfPWV) and central hemodynamics are independent cardiovascular risk factors and have the prognostic ability for CVD. We assessed cfPWV and central hemodynamics in young individuals with recent HIV infection diagnosis and without antiretroviral therapy. We hypothesized that individuals living with HIV would present greater cfPWV and central hemodynamics (central systolic blood pressure and pulse pressure) compared to uninfected controls. Methods. We recruited 51 treatment-naïve individuals living with HIV (HIV(+)) without previous CVD and 51 age- and sex-matched controls (HIV(-)). We evaluated traditional CVD risk factors including metabolic profile, blood pressure (BP), smoking, HIV viral load, and CD4 T-cell count. Arterial stiffness and central hemodynamics were evaluated by cfPWV, central systolic BP, and central pulse pressure (cPP) via applanation tonometry. Results. HIV(+) individuals presented a greater prevalence of smoking, reduced high-density lipoprotein cholesterol, and body mass index. 65.9% of HIV(+) individuals exhibited lymphocyte T CD4+ count cell/mm3. <500 cell/µL. There was no difference in brachial or central BP between groups; however, HIV(+) individuals showed significantly lower cPP. We observed a greater cfPWV (mean difference= 0.5 m/s; p<0.01) in HIV(+) compared to controls, even after adjusting for heart rate, mean arterial pressure and smoking. Conclusion: In the early stages of infection, non-treated HIV individuals present a greater prevalence of traditional CVD risk factors, arterial stiffness, and normal or decreased central hemodynamics.
The prognostic value of gender in gastric gastrointestinal stromal tumors: A propensity score matching analysis.
Authors: Rong Jian fang , Chen Si hai , Song Cong hua , Wang Huan , Zhao Qiao yun , Zhao Ru lin , He Ya jing , Yan Li li , Song Yan ping , Wang Fang fei , Xie Yong 
Published: June 2020

Abstract
Background: Gastrointestinal stromal tumors (GISTs) of the stomach are the most common GISTs. The risk, incidence and outcome of cancer are different between the sexes. Whether gender is related to the prognosis of gastric stromal tumors is unclear. Therefore, this study aims to explore the relationship between gender and gastric GIST prognosis.Methods: Data from gastric GIST patients were collected from the Surveillance, Epidemiology, and End Results (SEER) database. Propensity score matching (PSM) was performed to reduce confounding factors, and the clinicopathological features and prognosis of GIST patients were comprehensively evaluated.Results: There were 512 male patients and 538 female patients with gastric GIST. The gender of gastric GIST patients was associated with marital status, surgical treatment, tumor size, and mitotic index (P < 0.05). The Kaplan-Meier analysis and log-rank test revealed that male patients had a higher mortality rate than female patients (P = 0.0024). After matching all the potential confounding factors, the survival of the female gastric GIST patients was better than that of the male gastric GIST patients (P = 0.042). Cox regression analysis revealed that gender was an independent risk factor for overall survival. The risk of death was higher for males than for females (HR: 1.677, 95% CI: 1.150-2.444, P = 0.007). Conclusion: Gender could be a prognostic factor for gastric GIST survival, and male patients had a higher risk of death.
The Prevalence of antimicrobial resistance in Staphylococcus aureus and coagulase-negative Staphylococci strains isolated from inpatients
Authors: Farmehr Parva , fathi Javad , Hadadi Mahtab , motamedifar mohammad 
Published: June 2020

Abstract
Objectives: Globally nosocomial infection is a significant problem. Methicillin resistant Staphylococcus aureus (MRSA) and Methicillin resistant coagulase negative staphylococci (MRCoNS) are major causes of nosocomial infections. Aim of this study was to determine the prevalence and antimicrobial susceptibility of MRSA and MRCoNS in the southwest of Iran. This cross-sectional study was conducted on 221 non-duplicated staphylococci isolates collected from teaching hospital in Shiraz. The prevalence of MRSA and MRCoNS in clinical samples was identified with conventional microbiological tests. After identification, all of the isolates were subjected to antimicrobial susceptibility test and PCR to identify the presence of femA, mecA and pvl genes. Results: 70 (41.7 %) MRSA among 168 S. aureus, and 26 (15.48%) MRCoNS among 53 CoNS were examined. All of the isolates were susceptible to Trimethoprim/Sulfamethoxazole (100%). Chloramphenicol (65%) and Gentamicin (23%) were the other most active antibacterial agents against MRSA and MRCoNS. The frequency of pvl gene among S. aureus strains was 3.57%. There is need for developing the antibiotic policy and limiting the use of powerful antibiotics.
Semi field evaluation of freestanding transfluthrin passive emanators and the BG sentinel trap as a “Push-Pull control strategy” against Aedes aegypti mosquitoes
Authors: Tambwe Mgeni Mohamed , Moore Sarah , Chilumba Hassan , Swai Johnson Kyeba , Moore Jason , Stica Caleb , Saddler Adam 
Published: June 2020

Abstract
Background: Spatial repellents that drive mosquitoes away from treated areas, and odour-baited traps, that attract and kill mosquitoes, can be combined and work synergistically in apush-pull system. Push-pull systems have been shown to reduce house entry and outdoor biting rates of malaria vectors and so have the potential to control other outdoor biting mosquitoes such as Aedes aegypti that transmit arboviral diseases. In this study, semi-field experiments were conducted to evaluate whether a push-pull system could be used to reduce bites from Aedes mosquitoes. Methods: The push and pull under investigation consisted of two freestanding transfluthrin passive emanators (FTPE), and a BG sentinel trap (BGS) respectively. The FTPE contained hessian strips treated with 5.25g of transfluthrin active ingredient. The efficacies of FTPE and BGS alone and in combination were evaluated by human landing catch in a large semi-field system in Tanzania. We also investigated the protection of FTPE over six months. The data was analyzed using generalized linear mixed models with binomial distribution.Results: Two FTPE had a protective efficacy (PE) of 61. 2% (95% Confidence interval (CI): 52.2-69.9) against human landing rate of Aedes aegypti. The BGS did not significantly reduce mosquito landings; the PE was 2.1% (95% CI: -2.9-7.2). The combination of FTPE and BGS (push-pull) provided the PE of 64.5% (95% CI: 59.1-69.9). However, there was no significant difference in the protective efficacy between the push-pull and the two FTPE against Ae. aegypti (p=0.30). The FTPE offered significant protection against Ae. aegypti at month three, with a PE of 46.4% (95% CI: 41.1-51.8), but not at six months with a PE of 2.2 % (95% CI: -9.0-14.0). Conclusions: The PE of the FTPE and the full push-pull system are similar, indicative that bite prevention is primarily due to the activity of the FTPE. While these results are encouraging for the FTPE, further work is needed for a push-pull system to be recommended for Ae. aegypti control. The three-month protection provided by the FTPE against Ae. aegypti bites suggests it would be a useful additional personal protection tool during dengue outbreaks, that does not require regular user compliance.
Risk and predictors of in-hospital mortality from COVID-19 in patients with diabetes and cardiovascular disease
Authors: Rastad Hadith , Karim Hossein , Ejtahed Hanieh-Sadat , Tajbakhsh Ramin , Noorisepehr Mohammad , Babaei Mehrdad , Azimzadeh Mehdi , Soleimani Alireza , Inanloo Seyed Hasan , Hassani Neda Shafiabadi , Rasanezhad Fariba , Shahrestanaki Ehsan , Khodaparast Zeinab , Golami Hossein , Qorbani Mostafa 
Published: June 2020

Abstract
Background: Diabetes mellitus (DM) and cardiovascular disease (CVD) are present in a large number of patients with novel Coronavirus disease 2019 (COVID-19). We aimed to determine the risk and predictors of in-hospital mortality from COVID-19 in patients with DM and CVD.Methods: This retrospective cohort study included hospitalized patients aged ≥ 18 years with confirmed COVID-19 in Alborz province, Iran, from 20 February 2020 to 25 March 2020. Data on demographic, clinical and outcome (in-hospital mortality) data were obtained from electronic medical records. Self-reported comorbidities were classified into the following groups: “DM” (having DM with or without other comorbidities), “only DM” (having DM without other comorbidities), “CVD” (having CVD with or without other comorbidities), “only CVD” (having CVD without other comorbidities), and “having any comorbidity”. Multivariate logistic regression models were fitted to quantify the risk and predictors of in-hospital mortality from COVID-19 in patients with these comorbidities.Results: Among 2957 patients with COVID-19, 2656 were discharged as cured, and 301 died. In multivariate model, DM (OR: 1.62 (95%CI: 1.14-2.30)) and only DM (1.69 (1.05-2.74)) increased the risk of death from COVID-19; but, both CVD and only CVD showed non-significant associations (p>0.05). Moreover, “having any comorbidities” increased the risk of in-hospital mortality from COVID-19 (OR: 2.66 (95%CI: 2.09 -3.40)). Significant predictors of mortality from COVID-19 in patients with DM were lymphocyte count, creatinine and C-reactive protein (CRP) level (all P- values < 0.05).Conclusions: Our findings suggest that diabetic patients have an increased risk of in-hospital mortality following COVID-19; also, lymphocyte count, creatinine and CRP concentrations could be considered as significant predictors for the death of COVID-19 in these patients.
LncRNA BANCR Facilitates Melanoma Cells Growth, Migration and Invasion by Inhibiting miR-206 and miR-571 to Induce G6PD Expression
Authors: Yang Yuye , Yang Huixin , Yi Zihan , Yang Lijuan , Ni Yueli , Zhou Meiling , Xiong Guohang , AGBANA Yannick Luther , Zhao Lei , Yang Zhe , Zhang Qiao , Kuang Yingmin , Zhu Yuechun 
Published: June 2020

Abstract
Background Here, we evaluated the roles of LncRNA BANCR in facilitating melanoma cells growth, migration, invasion. Moreover, we examined the molecular mechanisms of BANCR in promoting melanoma development.Methods Here, we measured BANCR expression by quantitative PCR. By transwell assay, we detected the cancer cell migration and invasion. The melanoma growth and migration induced by BANCR was detected by colony formation assay and in xenograft nude mice model. By Western blotting and luciferase assay, we evaluated the molecular mechanism of BANCR in inhibiting miR-206 and miR-571 to induce G6PD expression. Results We found BANCR was overexpressed in melanoma cells and tissues. In addition, we showed that BANCR promoted melanoma cells growth in vitro and in vivo. Moreover, BANCR promotes cancer cell migration and invasion in vitro, and induced melanoma metastasis in nude mice model. The migration and invasion induced by BANCR in melanoma cells were involved in upregulation of matrix metalloproteinases (MMPs) MMP2 and MMP9. Mechanismly, we indicated BANCR promoted melanoma cell growth and migration by suppressing miR-206 and miR-571 expression to activate glucose metabolism pathway and induce G6PD expression. Furthermore, miR-206 and miR-571 inhibited G6PD expression by directly binding the 3’UTR of G6PD. We also showed miR-206 and miR-571 inhibited the proliferation of melanoma cells.Conclusion Our findings indicated that BANCR contributed to melanoma development, which might provide novel insights into the function of lncRNA-driven carcinogenesis in melanoma.
Determining Causes of Gully Erosion and Associated Rates of Change in South-east Nigeria, Using a Remote Sensing and GIS Methodology
Authors: Iro Sylvanus Ibeabuchi 
Published: June 2020

Abstract
In this work a study of gully erosion in southeast Nigeria is presented. The study of gully development on a regional scale is currently undermined by the inherent costs associated with consistent field monitoring and the lack of historic measurements to perform time series analysis. The analysis of study area topography at 30m resolution reveals 85% of the surveyed gullies develop on concave slopes with high values of 6 plan curvatures and >50 inclines. Results also reveal high association with ferralsols soils. Statistical analysis to determine significance of variables on the proportional yearly gully change in metre squared per square metre were conducted via principle component analysis. The analysis of this work was restricted to the time periods 2006/7, 2009/10, and 2014/15. The approach did not report any existence of one singular driver of erosion across the studied years and multiple sites confirming the complexity of gullies. The PCA showed that the level of variance explained in the yearly gully change variable was most similar in PC1 (representing the component with the highest eigenvalue) to Vegetation loss, Vegetation loss and slope in the respective years.The study offers a method of monitoring gully development from early stage to maturity and exemplifies the complexity and variability of erosion drivers in the SE Nigeria region. It presents a verified approach to local and regional monitoring of gullies, enacted through use of low budget/computing cost remote sensing and classification technologies, and serves to embolden civilian and governmental efforts to manage the societal and environmental menace of gully erosion.
Mapping Regional Forest Management Units: A Road-based Framework in Southeastern Coastal Plain and Piedmont
Authors: Yang Di , Fu Chiung-Shiuan 
Published: June 2020

Abstract
Management practices are one of the most important factors affecting forest structure and function. Landowners in southern United States manage forests using appropriately sized areas, to meet management objectives that include economic return, sustainability, and esthetic enjoyment. Road networks spatially designate the socio-environmental elements for the forests, which represented and aggregated as forest management units. Road networks are widely used for managing forests by setting logging roads and firebreaks. We propose that common types of forest management are practiced in road-delineated units that can be determined by remote sensing satellite imagery coupled with crowd-sourced road network datasets. Satellite sensors do not always capture road-caused canopy openings, so it is difficult to delineate ecologically relevant units based only on satellite data. By integrating citizen-based road networks with the National Land Cover Database, we mapped road-delineated management units across the regional landscape and analyzed the size frequency distribution of management units. We found the road-delineated units smaller than 0.5 Ha comprised 64% of the number of units, but only 0.98% of the total forest area. We also applied a statistical similarity test (Warren’s I) to access the equivalency of road-delineated units with forest disturbances by simulating a serious of neutral landscapes. The outputs showed that the whole southeastern U.S. has the probability of road-delineated unit of 0.44 and production forests overlapped significantly with disturbance areas with an average probability of 0.50.