bluuebunny/crossref_metadata_2025_split huggingface dataset:
Karakterisasi Bakteri Asam Laktat Proteolitik pada Bekasam
Authors: Wikandari Prima Retno , Suparmo Suparmo , Marsono Yustinus , Rahayu Endang Sutriswati 
Published: December 2019

Bekasam is a traditional fermented fish from Indonesia. It is made from fish in which the gills and gut had been discarded, washed, and mixed with salt, after two days, it was covered with rice and salt, and then fermented for 5-7 days at room temperature. Bekasam is perceived to have antihypertensive activity, which is though to be the activity of bioactive peptides, the product of protein degradation during the bekasam fermentation. The research was aim to isolate the indigenous lactic acid bacteria that produce strong proteolytic capabilities. The lactic acid bacteria were collected from 3 kinds of bekasam (tilapia, milkfish, and tuna bekasam). Isolation of the lactic acid bacteria was done on agar medium containing MRS + CaCO3, and the screening of the proteolytic bacteria was done by growing the isolated culture on skim agar. The colonies that produce clear zone were identified as proteolytic lactic acid bacteria. Some of 180 isolated strains of acid producing bacteria that produce clear zone on MRS agar were found 150 strains of lactic acid bacteria, and 84 strains showed their proteolytic activity. The later were identified morphologically and biochemical as Lactobacillus, Pediococcus and Leuconostoc genera. Selection was further conducted based on the height of the proteolytic and homofermentative fermentation activities. Upon species identification using API CH 50 kit, the selected strains belong to species of Lactobacillus plantarum, Lactobacillus pentosus, and Pediococcus pentosaseus.
Antioxidant and Antibacterial Activity of Plectaranthus amboinicus Leaf Extract
Authors: Islami Deri , Teruna Hilwan Yuda , Eryanti Yum 
Published: December 2019

Plectranthus amboinicus (Lour.) is a perennial plant belonging to the family of Lamiaceae which have been widely used in traditional herbal medicine. In the present study, the extracts of the leaves of P. amboinicus that was extracted by different solvents were evaluated for their antioxidant and antibacterial properties. The fresh leaves of the plant were extracted using subsequent methanol, n-hexane and dichloromethane. The antioxidant activity was determined by DPPH free radical scavenging assay and the antimicrobial activity was tested against Escherichia coli and Staphylococcus aureus by agar well diffusion assay. The results of antioxidant activity test showed that dichloromethane extract of the leaves had the highest antioxidant activity among all with IC50 value of 50.2 μg/mL Methanol and n-hexane extracts were still active in DPPH with IC50 value of 71.3 μg/mL and 205.9 μg/mL, respectively. Furthermore, the dichloromethane and methanol ektracts have shown a promising antibacterial activity in E. coli. The findings indicated that dichloromethane and methanol extracts of the leaves of P. amboinicus were potential further study as herbal medicine as they showed high antioxidant and antibacterial activities.
Fatigue life evaluation of composite wing spar cap materials
Authors: Trappe Volker 
Published: December 2019

AbstractAn effective method for evaluating the fatigue strength of thick unidirectional composite laminates of wing spar caps has been presented here. A typical width-tapered bending specimen has been developed for a four-point loading set up. Static and fatigue loading was performed at a load ratio R = ‒1. The complex stress state has been investigated numerically by finite element analysis. Finally, the concept is proven experimentally on specimens made of pultruded fibre rods. The fatigue behavior of the continuously tapered width by water jet cutting has been compared to discrete tapering via rod-drop. The new method enables a screening of material fatigue behavior caused by normal and shear loading.
Effect of inoculant type and treatment material quantity on properties of vermicular graphite cast iron rail vehicle brake discs
Authors: Er Onur , Kaluc Erdinc , Taban Emel , Ozsarac Ugur 
Published: December 2019

AbstractThis paper examines the effects of inoculant type and treatment material quantity on the mechanical and thermal properties of rail vehicle brake discs manufactured from vermicular graphite cast iron/compacted graphite iron (CGI). The in-mold treatment technique was employed in manufacturing the CGI brake discs. The main and interaction effects of inoculant type and treatment material quantity parameters affecting the tensile strength of the CGI brake discs were determined. The results indicate that treatment material quantity has the most significant effect on mechanical properties. Tensile strength was observed to increase as the treatment material quantity increased. The optimum level for manufacturing the CGI brake disc by using the in-mold treatment technique was attained via the combination of SB 5-type inoculant and 625 g treatment material quantity.
LIFE CYCLE COST ANALYSIS OF A PBC PILOT PROJECT FOR ROAD IN INDONESIA
Authors: Susanti Betty , Wirahadikusumah Reini D , Soemardi Biemo W , Sutrisno Mei 
Published: December 2019

Implementation of Performance-based Contracts (PBC) for road maintenance in Indonesia still requires various studies, especially related to potential long-term cost efficiencies that can be achieved by the road agencies by shifting the application of traditional contracts to PBC. This study assesses the effectiveness of PBC compared to traditional contract implementation based on a case study on one of the PBC pilot projects in Indonesia. The effectiveness of the PBC reviews in terms of project life cycle cost efficiency was calculated. LCC calculations were conducted by considering the influence of the length of contracted road, the initial conditions of contracted road, and the duration of PBC project. The LCC calculation shows that the implementation of PBC generally has the potential to generate LCC efficiency compared to the traditional contract. This study also proved that the implementation of PBC can guarantee the performance of road services during multi-year contracts and also provide other benefits for the road agency and road users.
ABSTRAK: Pelaksanaan Kontrak Berasaskan Prestasi (PBC) bagi penyelenggaraan jalan di Indonesia masih memerlukan pelbagai kajian, terutama berkaitan potensi keberkesanan perbelanjaan jangka panjang yang dapat dicapai oleh pihak agensi jalan dengan mengalihkan penerapan kontrak tradisional ke PBC. Kajian ini menilai keberkesanan PBC berbanding dengan pelaksanaan kontrak tradisional berdasarkan kajian kes pada salah satu projek perintis PBC di Indonesia. Keberkesanan ulasan PBC ini adalah dari segi kecekapan kos kitaran hayat projek. Pengiraan LCC dilakukan dengan mempertimbangkan pengaruh panjang jalan yang dikontrak, keadaan awal jalan terkontrak, dan jangka waktu projek PBC. Pengiraan LCC menunjukkan bahawa pelaksanaan PBC secara amnya berpotensi untuk menghasilkan kecekapan LCC berbanding dengan kontrak tradisional. Kajian ini juga membuktikan bahawa pelaksanaan PBC dapat menjamin prestasi perkhidmatan jalan selama kontrak multi-tahun dan juga memberi manfaat lain untuk agensi jalan dan pengguna jalan raya.
SPHINCTEROPLASTY IN THE TREATMENT OF ANAL INCONTINENCE ON THE BASIS OF NECROTIZING FASCIITIS
Authors: Kanikovskyi O. E. , Osadchyi A. V. , Kotsyura O. A. , Tomashevskyi A. V. , Zarezenko T. P. 
Published: December 2019

Incontinence of intestinal contents is a serious disease that gives patients strong emotional and psychological feelings leading to social self-isolation, unsuitability and disability of the population. From an analysis of scientific research, it is known that more than 30% of coloproctologic operations are complicated by insufficiency of the sphincter apparatus and lead to anal incontinence.
The purpose of the study - to improve the results of treatment for anal incontinence.
Material and methods. 44 patients with perineal NF were analyzed. Patients were treated in the surgical clinic of the Faculty of Medicine No. 2 of VNMU and Vinnitsa City Clinical Hospital of Emergency Medical Services from 2010 to 2018. The average age is 59.0±8.72 years (26-80). There are 11 women and 33 men. Age <20 - 0, 21-29 – 1 (2.27%), 30-39 – 2 (4.54%), 40-49 – 7 (15.9%), 50-59 – 10 (22.74%), >60 years old – 24 (54.5%).
Results and their discussion. The development of postoperative anal incontinence was identified in 13 (29,5%) patients. An anal incontinence scale (Wexner) was used to verify the diagnosis of anal canal failure. The surgical treatment technique was performed both in patiently in 4 (9.0%) patients and on an outpatient basis in 4 (9.0%). With cicatricial changes in the anal canal, the rumen was excised, the sphincter edges were mobilized, allograft implantation and sphincteroplasty were performed. Narrowing the anal canal is a less traumatic method that can be performed on an outpatient basis and in hospitals of one day. After surgery, patients independently retain gases and feces.
Conclusions. Patients with NF requiring immediate surgical treatment. Anal incontinence on the basis of the transferred NF leads to disability. The narrowing of the anal canal using a mesh transplant is minimally invasive, characterized by a mild postoperative course.Sphincteroplasty is possible to carry out stationary and on an outpatient basis under local anesthesia. Restoration of the function of the sphincter apparatus in 100% of the operated patients on the Wexner scale, which improves the level of physical and social life of patients.
ASSESSMENT OF ALCOHOL USE BY POPULATION AND RELATED PROBLEMS
Authors: Zamkevich V. B. , Diachuk M. D. , Gruzieva T. S. 
Published: December 2019

Introduction. The development of the public health system provides for the solution of the basic operational functions of public health, including surveillance and assessment of the state of health and well-being of the population, taking into account risks to public health.Given the importance of alcohol as a risk factor for the development of diseases, the study of its prevalence in the population, consumption patterns and health effects is an important public health problem.
The aim of the study. To identify current trends in alcohol consumption and the associated risk factor for health damage in Ukraine and countries of the WHO European Region.Materials and methods. Bibliographic, medical, statistical and information-analytical methods were used in the study. The key scientific base were the European Health for All Database, the Global Information System on Alcohol and Health (GISAH) and the European Information System on Alcohol and Health (EISAH).
Results. Significant differences were found in the prevalence of alcohol consumption by population in different regions of the world per capita per year, up to 16 times, with the highest rates in the WHO ER. Ukraine is characterized by high levels of alcohol consumption (8.6 liters) and negative tendencies for their growth during 1991-2016 by 45.8%. At the same time, in Ukraine the consumption of strong alcoholic drinks predominates, while in the countries of the European Union - wine and beer. The increase in alcohol consumption in Ukraine and the prevalence of strong drinks in the structure of consumed products (51.5%) is an unfavorable prognostic indicator and requires the adoption of preventive measures. The negative consequences of alcohol consumption are the prevalence of alcohol dependence (2.2%), alcohol-related disorders (6%), a significant proportion of deaths (20.5%) due to this risk factor in the overall structure of all deaths, etc. Significant is the contribution of alcohol to the formation of indicators of road traffic injuries, including those with a fatal outcome, crime rates, etc.
Conclusions. Surveillance of the prevalence of alcohol use, patterns of consumption and assessment of alcohol-related public health problems is an important basis for determining directions, justification and taking countermeasures.
DECREASED PAIN SCALE BASED ON CRITICAL PAIN OBSERVATION TOOL (CPOT) AND BEHAVIORAL PAIN SCALE NON-INTUBATED (BPS-NI) IN PATIENTS WITH REDUCED CONSCIOUSNESS BY CLASSICAL MUSIC INTERVENTION IN UNS HOSPITAL
Authors: Hanafi Muchtar , Muhammad Faizal , Wiyono Nanang , Saptiwi Betty 
Published: December 2019

Patient with reduced consciousness needs attention to their comfort as well as pain during treatment. Listening to classical music caused relaxation and pain reduction. This study aimed to determine the effect of classical music on the CPOT score, BPS-NI, and cortisol levels in patients with reduced consciousness. The research was conducted by using a pretest-posttest control group design. 18 patients were divided into the control group (C) and the classical music group (M). Classical music was played for 3 days respectively that consisted of 3 sessions per day. Furthermore, each patient was tested for cortisol levels on the third day. Comparative test of mean difference between pre-intervention and post-intervention obtained significant results in CPOT (p=Â 0.000) and BPS-NI (p=Â 0.001) measurements in all groups. Tukey's post-hoc test obtained significant results in which the M group had a lower mean score. The mean of cortisol level was higher in M group (14.7 Î¼g/dL) compared to the C group (12.96 Î¼g/dL), although not significant statistically (p=Â 0.67). Listening to classical music for patients with reduced consciousness decreased the pain scale. This was supported by the average of cortisol levels that were higher in the M group, considering that cortisol was a hormone that prevents pain.
COMPARATIVE HEMOGLOBIN AND HEMATOCRIT BEFORE AND AFTER DONATION TO BLOOD DONATE IN UNIT TRANSFUSION YOGYAKARTA CITY
Authors: Astuti Yuli , Artini Dyah 
Published: December 2019

Blood is one of the main components in the human body. The people's need for blood is high, therefore the blood service in Indonesia should have good quality and total services. Unit Transfusion Yogyakarta City is one of the good services in the blood donation. In the blood donation, examination hemoglobin and hematocrit are important to selection for donors.Â The aim of this study is to determine comparative in hemoglobin and hematocrit levels before and after donors in UTD PMI Yogyakarta City.Â The type of research used was experimental with cross sectional research design. The sampling technique used was accidental sampling with a total sample of 67 donors.Â Based on the results of statistical tests on the data obtained, the results obtained with p value less than 0,05 which is significantly different between the hemoglobin level before and after the donor.Â Based on these result there were differences in hemoglobin and hematocrit levels before and after of blood donation in Unit Transfusion PMI Yogyakarta City.
ANTIBACTERIAL ASSAY OF 2â€™-HYDROXY-4â€™,6â€™,4-TRIMETHOXYCHALCONE AND 4-METHOXYCHALCONE AGAINST GRAM POSITIVE AND GRAM NEGATIVE BACTERIA
Authors: Sepvianti Wiwit , Kusumaningrum Serafica Btari Christiyani 
Published: December 2019

Blood transfusion is currently one of the most important health care treatments. Unfortunately, the prevalence of transfusion reactions caused by the use of blood products is still quite high although blood products have been tested in terms of infectious diseases using 4 parameters, namely hepatitis B, hepatitis C, HIV and syphilis. The results of the analysis of blood products produced by UTD (Blood Transfusion Unit) Surabaya showed bacterial contamination of blood products, starting from normal skin flora to pathogens. Bacterial contamination is also believed to be one of the causes of transfusion reactions. Therefore, it is necessary to make efforts to prevent bacterial contamination of blood products. One of the efforts is to find chemical compounds that have good antibacterial activity.Â In this study,Â the antibacterial activity of 2Ëˆ-hydroxy-4Ëˆ,6Ëˆ,4-trimethoxychalcone and 4-methoxychalcone was tested against gram positive and gram negative bacteria. The test method was agar diffusion at various concentrations of chalcone, namely 0.625; 1.25; 2.5 and 5.0%. The results showed that both chalcones had good antibacterial activity against gram-positive and gram-negative bacteria.
Enzymatic hydrolysis on protein and β-glucan content of Sang-yod rice bran hydrolysates and their anti-inflammatory activity on RAW 264.7 cells
Authors: Phantuwong Natcha , Thongraung Chakree , Yupanqui Chutha Takahashi 
Published: December 2019

Background: Research focusing on the improvement of the utilization of rice bran is increasing due to its nutritional properties. Several biological activities of rice bran hydrolysates and its constituents have been reported. Sang-yod rice, a local rice variety in Southern of Thailand, is a pigmented rice. Furthermore, its bran has high nutritive value and health beneficial components. Accordingly, there is growing interest in transforming this by-product into a functional food ingredient.Objective: To investigate the effect of enzymatic hydrolysis processes on the digestion of protein and β-glucan and evaluate anti-proinflammatory properties of selected hydrolysates on RAW 264.7 macrophage cells.Method: Sang-yod rice bran hydrolysates were obtained using a single or co-enzymatic hydrolysis process and sequential hydrolysis process using amyloglucosidase and protease G6. Effects of enzyme concentration (3-5% v/w) and hydrolysis duration (30, 60, and 120 min) on soluble protein and β-glucan contents of obtained rice bran hydrolysates were evaluated. The selected rice bran hydrolysates were evaluated for their cell viability and inhibition against NO and pro-inflammatory cytokines generation on RAW 264.7 mouse macrophage cell lines. Results: Protein content (0.59-3.37 %) of the rice bran hydrolysates (RBHs) was increased by increasing of enzyme concentration (3-5% v/w) and hydrolysis time (60-120 min). However, the β-glucan content (0.88-4.63%) of RBHs decreased with the increase of those parameters. The RBHs derived by the sequential process using 5% v/w enzyme concentration and 60 min hydrolysis time gave high protein (3.23%) and high β-glucan (4.02%) contents. The hydrolysates with high amount of protein and/or β-glucan contents demonstrated no cytotoxicity against RAW 264.7 cells at concentration range of 100-2,000 μg/ml. Additionally, they demonstrated NO inhibition and pro-inflammatory inhibition ranges of 49.09-71.63% and 9.37-71.96% respectively. Generation of TNF-α, IL-6, and IL-1β cytokines was inhibited differently by the selected RBHs. Conclusion: Pre-digestion of Sang-yod rice bran with amyloglucosidase followed with co-hydrolysis of amyloglucosidase and protease G6 of the sequential hydrolysis process was the most effective process to release β-glucan and protein from of rice bran. The hydrolysate obtained from the process using enzyme concentration at 5%v/w and 60 min hydrolysis duration of each stage had the highest soluble β-glucan and protein content. Moreover, the process provided the hydrolysates with potential anti-inflammatory properties on nitric oxide inhibition and pro-inflammatory cytokines inhibition on RAW 264.7 macrophage cell line. Keywords: Sang-yod rice, Rice bran hydrolysate, β-glucan, Enzymatic hydrolysis, Anti-inflammatory activity
Daily consumption of fermented soymilk helps to improve facial wrinkles in healthy postmenopausal women in a randomized, parallel-group, open-label trial
Authors: Kano Mitsuyoshi , Haga Kazuyoshi , Miyazaki Kouji , Ishikawa Fumiyasu 
Published: December 2019

Background: Soymilk fermented by lactobacilli and/or bifidobacteria is attracting attention due to the excellent bioavailability of its isoflavones. We investigated the effects of fermented soymilk containing high amounts of isoflavone aglycones on facial wrinkles and urinary isoflavones in postmenopausal women in a randomized, parallel-group, open-label trial. Healthy Japanese women were randomly divided into active (n = 44, mean age 56.3 ± 0.5) or control (n = 44, mean age 56.1 ± 0.5) groups, who consumed or did not consume a bottle of soymilk fermented by Bifidobacterium breve strain Yakult and Lactobacillus mali for 8 weeks. Maximum depth of wrinkles around the crow’s feet area and other wrinkle parameters were evaluated as primary and secondary endpoints respectively at weeks 0, 4, and 8 during the consumption period. Urinary isoflavone levels were determined by liquid chromatography-mass spectrometry. Results: The active group demonstrated significant improvements in the maximum depth (p=0.015) and average depth (p=0.04) of wrinkles, and significantly elevated urinary isoflavones (daidzein, genistein, and glycitein; each p < 0.001) compared with the control during the consumption period. No serious adverse effects were recorded.Conclusion: These findings suggest that fermented soymilk taken daily may improve facial wrinkles and elevate urinary isoflavones in healthy postmenopausal women.Key words: postmenopausal women; isoflavone; fermented soymilk; phytoestrogen; facial wrinkle
Continuous cyclic compressive loading modulates biological and mechanical properties of collagen hydrogels seeded with human chondrocytes
Authors: Nebelung Sven , Gavenis Karsten , Rath Björn , Tingart Marcus , Ladenburger Andreas , Stoffel Marcus , Zhou Bei , Mueller-Rath Ralf 
Published: December 2019

Purpose: This study investigated the potential of cyclic compressive loading in the generation of in vitro engineered cartilaginous tissue with the aim of contributing to a better understanding of mechanical preconditioning and its possible role in further optimizing existing matrix-associated cartilage replacement procedures. Methods: Human chondrocytes were harvested from 12 osteoarthritic knee joints and seeded into a type I collagen (col-I) hydrogel at low density (2×105 cells/ml gel). The cell-seeded hydrogel was condensed and cultivated under continuous cyclic compressive loading (frequency: 0.3 Hz; strain: 10%) for 14 days under standardized conditions. After retrieval, specimens were subject to staining, histomorphometric evaluation, gene expression analysis and biomechanical testing. Results: Cellular morphology was altered by both stimulation and control conditions as was staining for collagen II (col-II). Gene expression measurements revealed a significant increase for col-II under either cultivation condition. No significant differences in col-I, aggrecan and MMP-13 gene expression profiles were found. The col-II/col-I mRNA ratio significantly increased under stimulation, whereas the biomechanical properties deteriorated under either cultivation method. Conclusions: Although the effects observed are small, mechanical preconditioning has demonstrated its potential to modulate biological properties of collagen hydrogels seeded with human chondrocytes.