bluuebunny/crossref_metadata_2025_split huggingface dataset:
Host identity is more important in structuring bacterial epiphytes than endophytes in a tropical mangrove forest
Authors: Yao Hui , Sun Xiang , He Chao , Li Xing-Chun , Guo Liang-Dong 
Published: March 2020

ABSTRACTInteractions between plants and microbes are involved in biodiversity maintenance, community stability and ecosystem functioning. However, differences in the community and network structures between phyllosphere epiphytic and endophytic bacteria have rarely been investigated. Here, we examined phyllosphere epiphytic and endophytic bacterial communities of six mangrove species using Illumina MiSeq sequencing of the 16S rRNA gene. The results revealed that the community structure of epiphytic and endophytic bacteria was different. Plant identity significantly affected the diversity and community structure of both epiphytic and endophytic bacteria, with a greater effect on the community structure of the former than the latter. Network analysis showed that both plant–epiphytic and plant–endophytic bacterial network structures were characterized by significantly highly specialized and modular but lowly connected and anti-nested properties. Furthermore, the epiphytic bacterial network was more highly specialized and modular but less connected and more strongly anti-nested than the endophytic bacterial network. This study reveals that the phyllosphere epiphytic and endophytic bacterial community structures differ and plant identity has a greater effect on the epiphytic than on the endophytic bacteria, which may provide a comprehensive insight into the role of plant identity in driving the phyllosphere epiphytic and endophytic microbial community structures in mangrove ecosystems.
Structure and distribution of nitrite-dependent anaerobic methane oxidation bacteria vary with water tables in Zoige peatlands
Authors: Zhong Qiuping , Xue Dan , Chen Huai , Liu Liangfeng , He Yixin , Zhu Dan , He Zhili 
Published: March 2020

ABSTRACT
The recently discovered nitrite-dependent anaerobic methane oxidation (n-damo) is an important methane sink in natural ecosystems performed by NC10 phylum bacteria. However, the effect of water table (WT) gradient due to global change on n-damo bacterial communities is not well studied in peatlands. Here, we analysed the vertical distribution (0–100 cm) of n-damo bacterial communities at three sites with different WTs of the Zoige peatlands in the Qinghai-Tibetan Plateau. Using an n-damo bacterial specific 16S rRNA gene clone library, we obtained 25 operational taxonomic units (OTUs) that could be divided into Groups A, B, C, D and E (dominated by A and B). The dominant group was Group B at the high (OTU14 and OTU20) and intermediate (OTU7 and OTU8) WT sites and Group A was dominant at the low WT site (OTU6 and OTU5). Using high-throughput sequencing, we observed that n-damo bacteria mainly distributed in subsurface soils (50–60 and 20–30 cm), and their relative abundances were higher at the low WT site than at the other two sites. In addition, we found that pH and nitrate were positively correlated with Group A, while total organic carbon, total nitrogen and ammonia were positively associated with Group B. Our study provides new insights into our understanding of the response of n-damo bacteria to WT gradient in peatlands, with important implications for global change.
Modulation of insect-induced oxidative stress responses by microbial fertilizers in Brassica juncea
Authors: Sharma Garima , Mathur Vartika 
Published: March 2020

ABSTRACTMicrobial fertilizers have increasingly gained popularity as environmentally sustainable nutritional supplement for plant growth. However, the effect of these microbes on plant-induced responses and the resultant effect on late-arriving herbivores are still unclear. Following insect herbivory, oxidative stress is one of the earliest responses induced in plants. Therefore, we analyzed the effect of phosphorus solubilizing bacteria (PSB), vesicular-arbuscular mycorrhiza (VAM) and their combination on oxidative stress in Brassica juncea against Spodoptera litura herbivory. Six antioxidant enzymes, viz. superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), ascorbate peroxidase (APX), monodehydroascorbate reductase and glutathione reductase (GR), were studied. Our results indicated a sporadic increase of APX and GR in PSB-supplemented plants. In contrast, VAM-supplemented plants showed an active systemic response against herbivory with an increase in all the six enzymes at 72 h. Conversely, supplementation of PSB–VAM together led to increased APX, SOD, CAT and POD enzymes, which subsided by 72 h. Thus, the presence of VAM, alone or in combination with PSB, acted like a vaccination for plants against stress. However, the mode of action of PSB and VAM governed the temporal dynamics of antioxidants. Our study thus shows microbial fertilizers have prominent effects on plant immunity.
Emotion Recognition and Reactivity in Persons With Neurodegenerative Disease Are Differentially Associated With Caregiver Health
Authors: Brown Casey L , Wells Jenna L , Hua Alice Y , Chen Kuan-Hua , Merrilees Jennifer , Miller Bruce L , Levenson Robert W 
Published: March 2020

AbstractBackground and ObjectivesMotivated by the high rates of health problems found among caregivers of persons with neurodegenerative disease, we examined associations between deficits in two aspects of care recipients’ socioemotional functioning and their caregivers’ health.Research Design and MethodsIn 2 studies with independent samples (N = 171 and 73 dyads), caregivers reported on care recipients’ emotion recognition and emotional reactivity. Caregiver health was assessed using both self-report measures (Studies 1 and 2) and autonomic nervous system indices (Study 2).ResultsLower emotion recognition in care recipients was linearly associated with worse self-reported health, faster resting heart rate, and greater physiological reactivity to an acoustic startle stimulus in caregivers. These effects held after accounting for a variety of risk factors for poor caregiver health, including care recipients’ neuropsychiatric symptoms. Emotional reactivity showed a quadratic association with health, such that the lowest and highest levels of emotional reactivity in care recipients were associated with lower self-reported health in caregivers.Discussion and ImplicationsResults shed light on the unique associations between two aspects of care recipients’ emotional functioning and caregivers’ health. Findings suggest potential ways to identify and help caregivers at heightened risk for adverse health outcomes.
Meta-analysis of epilepsy prevalence in West Africa and its relationship with onchocerciasis endemicity and control
Authors: Siewe Fodjo Joseph Nelson , Remme Jan H F , Preux Pierre-Marie , Colebunders Robert 
Published: March 2020

Abstract

Background
A high prevalence and incidence of epilepsy has been reported in onchocerciasis-endemic regions in Central and East Africa. There is compelling epidemiological evidence suggesting that this high burden is caused by onchocerciasis-associated epilepsy (OAE). We hypothesized that OAE had also occured in West African onchocerciasis foci.

Methods
We searched PubMed, the African Journals Online platform and grey literature for population-based epilepsy studies in West African countries. Epilepsy and onchocerciasis prevalence data were extracted. The pre-control onchocerciasis endemicity in the study sites was estimated from historical data of onchocerciasis control programmes. The prevalence of epilepsy in different sites was analysed, taking into account onchocerciasis endemicity and the duration of control.

Results
The pooled prevalence of epilepsy in the West African study sites was 13.14 per 1000 (95% confidence interval 11.28–15.00). Higher pre-control endemicity and a shorter duration of onchocerciasis control were both associated with increased epilepsy prevalence (p&lt;0.001). Two studies in Ivory Coast that provided detailed descriptions of persons with epilepsy in onchocerciasis-endemic settings revealed that most of them had features of OAE (73.7% and 83.3%, respectively).

Conclusions
Our findings suggest that before and during the early years of implementing onchocerciasis control in West Africa, high onchocerciasis endemicity resulted in a high prevalence of OAE and that subsequent control efforts significantly reduced the prevalence of OAE.
Iatrogenic Thermal Burns Secondary to Marine Sting Treatment
Authors: Lewis Christopher J , Wood Fiona , Goodwin-Walters Anna 
Published: March 2020

AbstractHot water immersion (HWI) therapy is an effective and validated treatment for a variety of marine stings. Unsupervised, however, it poses a significant risk of thermal injury. Herein, we describe our experience of iatrogenic thermal injury secondary to marine sting treatment. A 5-year retrospective review of all iatrogenic thermal burns secondary to marine stings referred to the State Adult Burn Service was conducted. Nine patients were identified, all sustaining stings to the feet from estuarine cobblerfish, stonefish, and stingrays. All patients continued unsupervised HWI at home and sustained thermal injury to their feet. The majority were treated conservatively with dressings and elevation. One patient required surgical debridement. While heat application is an effective treatment for marine stings, further patient education is required following discharge from point of care. We recommend that first-aid treatment guidelines be updated to reflect that patients are not recommended to continue scalding water immersion at home. However, if patients wish to continue HWI, water temperature should be checked manually with a thermometer or with a nonstung limb and limited to 30 minutes immersion, with 30-minute skin recovery time between.
Cybersecurity, Technological Neutrality, and International Trade Law
Authors: Gagliani Gabriele 
Published: March 2020

ABSTRACT
There have already been several studies focusing on cybersecurity and international trade but the intersection between the two is multifaceted and can be approached from several viewpoints. This article focuses on cybersecurity and international trade from the specific perspective of technological neutrality. Although technological neutrality is recognized with different degrees of intensity both under World Trade Organization Covered Agreements and free trade agreements in a diverse range of fields (such as trade in services, technical barriers to trade, or intellectual property), its status in international trade law is unclear. In this uncertain context, it is argued here, technological neutrality has the potential of expanding the scope of trade obligations unpredictably. As a result, in the face of pressing cybersecurity concerns, technology-related trade measures risk to constantly violate trade obligations, making the trade-cybersecurity relationship even more complicated. The possibility to clarify the status of technological neutrality and the scope of technology-neutral provisions is chief among the solutions proposed in this article. Additionally, this article suggests for States either to be compensated when a trade-restrictive cybersecurity measure affects them, or to consider adopting a waiver in the field of technology, similar to what has been carried out in other areas.
Genetic identity concerns in the regulation of novel reproductive technologies
Authors: Ludlow Karinne 
Published: March 2020

Abstract
Confusion concerning a child’s genetic identity is a common objection to the application of novel technologies to human embryos. Unsurprisingly then, concern for genetic identity has been used to justify successive waves of regulatory activity and is again appearing in debates about regulatory responses to emerging reproductive technologies. By examining the history of Australian law’s understanding and responses to so-called genetic identity in the context of past and current scientific developments in reproductive technology, this paper investigates regulatory reform needed for still emerging reproductive technologies. While this paper presents and analyzes in some detail Australian regulation and the policy deliberations and scientific developments that led to them, the identified inconsistencies in the regulatory responses, and recommended reforms to address emerging reproductive technologies offered, address issues relevant to many countries responding to the same technologies.
Evaluating the Utility of Polygenic Risk Scores in Identifying High-Risk Individuals for Eight Common Cancers
Authors: Jia Guochong , Lu Yingchang , Wen Wanqing , Long Jirong , Liu Ying , Tao Ran , Li Bingshan , Denny Joshua C , Shu Xiao-Ou , Zheng Wei 
Published: March 2020

AbstractBackgroundGenome-wide association studies have identified common genetic risk variants in many loci associated with multiple cancers. We sought to systematically evaluate the utility of these risk variants in identifying high-risk individuals for eight common cancers.MethodsWe constructed polygenic risk scores (PRS) using genome-wide association studies–identified risk variants for each cancer. Using data from 400 812 participants of European descent in a population-based cohort study, UK Biobank, we estimated hazard ratios associated with PRS using Cox proportional hazard models and evaluated the performance of the PRS in cancer risk prediction and their ability to identify individuals at more than a twofold elevated risk, a risk level comparable to a moderate-penetrance mutation in known cancer predisposition genes.ResultsDuring a median follow-up of 5.8 years, 14 584 incident case patients of cancers were identified (ranging from 358 epithelial ovarian cancer case patients to 4430 prostate cancer case patients). Compared with those at an average risk, individuals among the highest 5% of the PRS had a two- to threefold elevated risk for cancer of the prostate, breast, pancreas, colorectal, or ovary, and an approximately 1.5-fold elevated risk of cancer of the lung, bladder, or kidney. The areas under the curve ranged from 0.567 to 0.662. Using PRS, 40.4% of the study participants can be classified as having more than a twofold elevated risk for at least one site-specific cancer.ConclusionsA large proportion of the general population can be identified at an elevated cancer risk by PRS, supporting the potential clinical utility of PRS for personalized cancer risk prediction.
Endovascular aneurysm repair in a centenarian: case report and systematic literature review
Authors: Sheikh Zara , Crockett Stephen , Selvakumar Sadasivam 
Published: March 2020

Abstract
The prevalence of abdominal aortic aneurysms (AAA) in the nonagenarian and centenarian populations is set to increase. Endovascular aneurysm repair (EVAR) has been shown to be achievable with excellent outcomes in carefully selected nonagenarians. However, experience with centenarians is limited. We report the case of a 100-year-old who presented with a tender 8-cm AAA and successfully underwent EVAR. This report describes the second case of AAA repair in a centenarian in the literature and the first reported EVAR in this demographic. The patient survived for 2 years after the procedure, was free of EVAR or aneurysm-related complications. Furthermore, we present a systematic review of the existing literature and insights pertaining to outcomes in nonagenarians undergoing EVAR.
Giant phyllodes tumor occupying the whole breast in a young female: feasibility of mastectomy and immediate reconstruction
Authors: Kolia Aikaterini K , Kalles Vasileios , Zografos Constantinos G , Chamzin Alexandros , Michalopoulos Nikolaos V 
Published: March 2020

Abstract
Giant phyllodes tumors are rare fibroepithelial breast neoplasms typically &gt;10 cm by definition. The best investigation for preoperative diagnosis is core biopsy, although it is often difficult for the pathologist to distinguish fibroadenomas from phyllodes tumors and determine the level of malignant potential. The wide excision is the appropriate therapy for phyllodes tumors taking into consideration that mastectomy may be required for huge phyllodes tumors. In the modern era of breast tissue expanders and breast implants, immediate breast reconstruction is feasible. We present a case report of a patient with a giant phyllodes tumor of 24 cm diameter who underwent mastectomy and immediate breast reconstruction with breast tissue expander.
SMA observations of Haro 2: molecular gas around a hot superbubble
Authors: Beck Sara C , Hsieh Pei-Ying , Turner Jean 
Published: March 2020

ABSTRACT
Haro 2, a nearby dwarf starburst dwarf galaxy with strong Ly α emission, hosts a starburst that has created outflows and filaments. The clear evidence for galactic outflow makes it an ideal candidate for studying the role of molecular gas in feedback processes in a dwarf galaxy. We observed CO(2–1) in Haro 2 at the Submillimeter Array in the compact and extended configurations, and have mapped the molecular emission with velocity resolution 4.1 km s−1 and spatial resolution 2.0 × 1.6 arcsec2. With this significant increase of resolution over previous measurements, we see that the molecular gas comprises two components: bright clumps associated with the embedded star clusters of the starburst, and fainter extended emission east of the starburst region. The extended emission coincides with an X-ray bubble and has the kinematic signatures of an outflowing cone or of an expanding shell or bubble; the velocity range is ∼35 km s−1. We suggest that the starburst winds that created the X-ray bubble have entrained the molecular gas, and that the apparent velocity gradient at an angle to the photometric axis is an artefact caused by the outflow. The molecular and X-ray activity is on the east of the galaxy and the ionized outflow and optical filaments are west; their relationship is not clear.
Finding binary black holes in the Milky Way with LISA
Authors: Sesana Alberto , Lamberts Astrid , Petiteau Antoine 
Published: March 2020

ABSTRACT
We determine the main properties of the Galactic binary black hole (BBH) population detectable by Laser Interferometer Space Antenna (LISA) and strategies to distinguish them from the much more numerous white dwarf binaries. We simulate BBH populations based on cosmological simulations of Milky Way-like galaxies and binary evolution models. We then determine their gravitational wave emission as observed by LISA and build mock catalogues. According to our model, LISA will detect ≈4 (6) BBHs assuming 4 (10) yr of operations. Those figures grow to ≈6 (9) when models are re-normalized to the inferred LIGO/Virgo merger rates. Largely independent on mass and distance, sources emitting at f &gt; 0.5 mHz – 40 per cent (70 per  cent) of the detections – have a measurable frequency drift, which allows a good enough chirp mass measurement to separate them from the much lighter white dwarf and neutron star binaries. Most of the remaining, lower frequency, sources should be identifiable by their lack of electromagnetic (EM) counterpart within ≈100 pc. These results are robust with respect to the current uncertainties of the BBH merger rate as measured by LIGO/Virgo as well as the global mass spectrum of the binaries. Based on the LIGO/Virgo merger rate, we determine that there is a 94 per  cent chance that LISA finds at least one of these systems within 4 yr, which will allow us to pinpoint the conditions where they were formed and possibly find unique EM signatures.
Actinomycin D downregulates Sox2 and improves survival in preclinical models of recurrent glioblastoma
Authors: Taylor Jessica T , Ellison Stuart , Pandele Alina , Wood Shaun , Nathan Erica , Forte Gabriella , Parker Helen , Zindy Egor , Elvin Mark , Dickson Alan , Williams Kaye J , Karabatsou Konstantina , McCabe Martin , McBain Catherine , Bigger Brian W 
Published: March 2020

Abstract

Background
Glioblastoma (GBM) has been extensively researched over the last few decades, yet despite aggressive multimodal treatment, recurrence is inevitable and second-line treatment options are limited. Here, we demonstrate how high-throughput screening (HTS) in multicellular spheroids can generate physiologically relevant patient chemosensitivity data using patient-derived cells in a rapid and cost-effective manner. Our HTS system identified actinomycin D (ACTD) to be highly cytotoxic over a panel of 12 patient-derived glioma stemlike cell (GSC) lines. ACTD is an antineoplastic antibiotic used in the treatment of childhood cancers. Here, we validate ACTD as a potential repurposed therapeutic for GBM in 3-dimensional GSC cultures and patient-derived xenograft models of recurrent glioblastoma.

Methods
Twelve patient-derived GSC lines were screened at 10 µM, as multicellular spheroids, in a 384-well serum-free assay with 133 FDA-approved compounds. GSCs were then treated in vitro with ACTD at established half-maximal inhibitory concentrations (IC50). Downregulation of sex determining region Y–box 2 (Sox2), a stem cell transcription factor, was investigated via western blot and through immunohistological assessment of murine brain tissue.

Results
Treatment with ACTD was shown to significantly reduce tumor growth in 2 recurrent GBM patient-derived models and significantly increased survival. ACTD is also shown to specifically downregulate the expression of Sox2 both in vitro and in vivo.

Conclusion
These findings indicate that, as predicted by our HTS, ACTD could deplete the cancer stem cell population within the tumor mass, ultimately leading to a delay in tumor progression.

Key Points
1. High-throughput chemosensitivity data demonstrated the broad efficacy of actinomycin D, which was validated in 3 preclinical models of glioblastoma.
2. Actinomycin D downregulated Sox2 in vitro and in vivo, indicating that this agent could target the stem cell population of GBM tumors.
A Systematic Review of the Association Between Perceived Injustice and Pain-Related Outcomes in Individuals with Musculoskeletal Pain
Authors: Carriere Junie S , Donayre Pimentel Stephania , Yakobov Esther , Edwards Robert R 
Published: March 2020

AbstractObjectiveA growing body of literature shows that justice-related appraisals are significant determinants of pain-related outcomes and prolonged trajectories of recovery. We conducted a systematic review of the literature assessing the relationship between perceived injustice and pain-related outcomes in individuals with musculoskeletal pain.Design and ParticipantsA search of published studies in English in PubMed, PsychInfo, Embase, and Cochrane Database of Systematic Reviews from database inception through May 2019 was performed. Search terms included “perceived injustice,” “injustice appraisals,” “perceptions of injustice,” and “pain” or “injury.”ResultsThirty-one studies met inclusion criteria. Data for a total of 5,969 patients with musculoskeletal pain were extracted. Twenty-three studies (71.9%) reported on individuals with persistent pain lasting over three months, and 17 studies (53.1%) reported on individuals with injury-related musculoskeletal pain. Significant associations were found between perceived injustice and pain intensity, disability and physical function, symptoms of depression and anxiety, post-traumatic stress disorder, quality of life and well-being, and quality of life and social functioning.ConclusionsThis systematic review summarizes the current evidence for the association between perceived injustice and pain-related outcomes. There is strong evidence that perceived injustice is associated with pain intensity, disability-related variables, and mental health outcomes. Implications and directions for future research are discussed.