bluuebunny/crossref_metadata_2025_split huggingface dataset:
Influence of MUC5B gene on antisynthetase syndrome
Authors: López-Mejías Raquel , Remuzgo-Martínez Sara , Genre Fernanda , Pulito-Cueto Verónica , Rozas Sonia M. Fernández , Llorca Javier , Fernández David Iturbe , Cuesta Víctor M. Mora , Ortego-Centeno Norberto , Gómez Nair Pérez , Mera-Varela Antonio , Martínez-Barrio Julia , López-Longo Francisco Javier , Mijares Verónica , Lera-Gómez Leticia , Usetti María Piedad , Laporta Rosalía , Pérez Virginia , Gafas Alicia De Pablo , González María Aránzazu Alfranca , Calvo-Alén Jaime , Romero-Bueno Fredeswinda , Sanchez-Pernaute Olga , Nuno Laura , Bonilla Gema , Balsa Alejandro , Hernández-González Fernanda , Grafia Ignacio , Prieto-González Sergio , Narvaez Javier , Trallero-Araguas Ernesto , Selva-O’Callaghan Albert , Gualillo Oreste , Castañeda Santos , Cavagna Lorenzo , Cifrian José M. , González-Gay Miguel A. 
Published: January 2020

AbstractMUC5B rs35705950 (G/T) is strongly associated with idiopathic pulmonary fibrosis (IPF) and also contributes to the risk of interstitial lung disease (ILD) in rheumatoid arthritis (RA-ILD) and chronic hypersensitivity pneumonitis (CHP). Due to this, we evaluated the implication of MUC5B rs35705950 in antisynthetase syndrome (ASSD), a pathology characterised by a high ILD incidence. 160 patients with ASSD (142 with ILD associated with ASSD [ASSD-ILD+]), 232 with ILD unrelated to ASSD (comprising 161 IPF, 27 RA-ILD and 44 CHP) and 534 healthy controls were genotyped. MUC5B rs35705950 frequency did not significantly differ between ASSD-ILD+ patients and healthy controls nor when ASSD patients were stratified according to the presence/absence of anti Jo-1 antibodies or ILD. No significant differences in MUC5B rs35705950 were also observed in ASSD-ILD+ patients with a usual interstitial pneumonia (UIP) pattern when compared to those with a non-UIP pattern. However, a statistically significant decrease of MUC5B rs35705950 GT, TT and T frequencies in ASSD-ILD+ patients compared to patients with ILD unrelated to ASSD was observed. In summary, our study does not support a role of MUC5B rs35705950 in ASSD. It also indicates that there are genetic differences between ILD associated with and that unrelated to ASSD.
Wheat dwarfing influences selection of the rhizosphere microbiome
Authors: Kavamura Vanessa N. , Robinson Rebekah J. , Hughes David , Clark Ian , Rossmann Maike , Melo Itamar Soares de , Hirsch Penny R. , Mendes Rodrigo , Mauchline Tim H. 
Published: January 2020

AbstractThe development of dwarf wheat cultivars combined with high levels of agrochemical inputs during the green revolution resulted in high yielding cropping systems. However, changes in wheat cultivars were made without considering impacts on plant and soil microbe interactions. We studied the effect of these changes on root traits and on the assembly of rhizosphere bacterial communities by comparing eight wheat cultivars ranging from tall to semi-dwarf plants grown under field conditions. Wheat breeding influenced root diameter and specific root length (SRL). Rhizosphere bacterial communities from tall cultivars were distinct from those associated with semi-dwarf cultivars, with higher differential abundance of Actinobacteria, Bacteroidetes and Proteobacteria in tall cultivars, compared with a higher differential abundance of Verrucomicrobia, Planctomycetes and Acidobacteria in semi-dwarf cultivars. Predicted microbial functions were also impacted and network analysis revealed a greater level of connectedness between microbial communities in the tall cultivars relative to semi-dwarf cultivars. Taken together, results suggest that the development of semi-dwarf plants might have affected the ability of plants to recruit and sustain a complex bacterial community network in the rhizosphere.
Selective plasma exchange in ABO-incompatible kidney transplantation: comparison of substitution with albumin and partial substitution with fresh frozen plasma
Authors: Hanaoka Ako , Naganuma Toshihide , Kabata Daijiro , Takemoto Yoshiaki , Uchida Junji , Nakatani Tatsuya , Shintani Ayumi 
Published: January 2020

AbstractWe have performed selective plasma exchange (SePE) as apheresis before ABO-incompatible kidney transplantation since 2015. In this study, we divided the SePE sessions into two groups, those using albumin alone (Group A) and those partially using fresh frozen plasma (FFP) (Group F), and compared their clinical efficacies. A total of 58 sessions of SePE (Group A: n = 41, Group F: n = 17) were performed in 30 recipients of ABOi kidney transplantation during the study period and the decrease in isoagglutinin titers, changes in the levels of serum IgG and IgM as well as coagulation factors (fibrinogen, factor XIII), and incidence of side effects were retrospectively compared. There was a more significant decrease of isoagglutinin titers in Group F compared to Group A. Immunoglobulins and coagulants were replenished in Group F. Meanwhile, the incidence of side effects was significantly higher in Group F. SePE using FFP, which can effectively decrease isoagglutinins titers and replenish immunoglobulin and coagulation factors, may be a beneficial treatment modality as apheresis before ABO-incompatible kidney transplantation, in spite of a disadvantage that there are many side effects.
Weighing the role of skeletal muscle mass and muscle density in cancer patients receiving PD-1/PD-L1 checkpoint inhibitors: a multicenter real-life study
Authors: Cortellini Alessio , Bozzetti Federico , Palumbo Pierpaolo , Brocco Davide , Di Marino Pietro , Tinari Nicola , De Tursi Michele , Agostinelli Veronica , Patruno Leonardo , Valdesi Cristina , Mereu Manuela , Verna Lucilla , Lanfiuti Baldi Paola , Venditti Olga , Cannita Katia , Masciocchi Carlo , Barile Antonio , McQuade Jennifer Leigh , Ficorella Corrado , Porzio Giampiero 
Published: January 2020

AbstractSarcopenia represents one of the hallmarks of all chronic diseases, including cancer, and was already investigated as a prognostic marker in the pre-immunotherapy era. Sarcopenia can be evaluated using cross-sectional image analysis of CT-scans, at the level of the third lumbar vertebra (L3), to estimate the skeletal muscle index (SMI), a surrogate of skeletal muscle mass, and to evaluate the skeletal muscle density (SMD). We performed a retrospective analysis of consecutive advanced cancer patient treated with PD-1/PD-L1 checkpoint inhibitors. Baseline SMI and SMD were evaluated and optimal cut-offs for survival, according to sex and BMI (+/−25) were computed. The evaluated clinical outcomes were: objective response rate (ORR), immune-related adverse events (irAEs), progression free survival (PFS) and overall survival (OS). From April 2015 to April 2019, 100 consecutive advanced cancer patients were evaluated. 50 (50%) patients had a baseline low SMI, while 51 (51%) had a baseline low SMD according to the established cut offs. We found a significant association between SMI and ECOG-PS (p = 0.0324), while no correlations were found regarding SMD and baseline clinical factors. The median follow-up was 20.3 months. Patients with low SMI had a significantly shorter PFS (HR = 1.66 [95% CI: 1.05–2.61]; p = 0.0291) at univariate analysis, but not at the multivariate analysis. They also had a significantly shorter OS (HR = 2.19 [95% CI: 1.31–3.64]; p = 0.0026). The multivariate analysis confirmed baseline SMI as an independent predictor for OS (HR = 2.19 [1.31–3.67]; p = 0.0027). We did not find significant relationships between baseline SMD and clinical outcomes, nor between ORR, irAEs and baseline SMI (data not shown). Low SMI is associated with shortened survival in advanced cancer patients treated with PD1/PDL1 checkpoint inhibitors. However, the lack of an association between SMI and clinical response suggests that sarcopenia may be generally prognostic in this setting rather than specifically predictive of response to immunotherapy.
Geologic factors leadingly drawing the macroecological pattern of rocky desertification in southwest China
Authors: Jiang Miao , Lin Yi , Chan Ting On , Yao Yunjun , Zheng Guang , Luo Shezhou , Zhang Lin , Liu Daping 
Published: January 2020

AbstractRocky desertification (RD) is a special process of land deterioration in karst topography, with a view of bedrock exposure and an effect of ecological degradation. Among the three largest karst regions in the world, southwest China boasts the largest RD area and highest diversity of karst landscapes. However, inefficient field surveying tends to restrict earlier studies of RD to local areas, and the high complexity of karst geomorphology in southwest China further lead to the shortage of the knowledge about its macroecological pattern so far. To address this gap, this study innovatively took county as the unit to statistically explore the links between the 2008-censused distributions of county-level RD in southwest China and its potential impact factors of three kinds (geologic, climatic, and anthropogenic), all transformed into the same mapping frame. Spatial pattern analyses based on spatial statistics and artificial interpretation unveiled the macroscopic characteristics of RD spatial patterns, and attribution analyses based on correlation analysis and dominance analysis exposed the links of the impact factors to RD and their contributions in deciding the macroscopic pattern of RD. The results suggested that geologic factors play a first role in drawing the macroecological pattern of RD, also for the slight-, moderate-, and severe-level RD scenarios, in southwest China. Despite this inference somehow collides with the popular awareness that anthropogenic factors like human activities are leadingly responsible for the RD-relevant losses, the findings are of practical implications in guiding making the macroscopic policies for mitigating RD degradation and advancing its environmental restoration.
Responses to addiction help-seeking from Alexa, Siri, Google Assistant, Cortana, and Bixby intelligent virtual assistants
Authors: Nobles Alicia L. , Leas Eric C. , Caputi Theodore L. , Zhu Shu-Hong , Strathdee Steffanie A. , Ayers John W. 
Published: January 2020

AbstractWe investigated how intelligent virtual assistants (IVA), including Amazon’s Alexa, Apple’s Siri, Google Assistant, Microsoft’s Cortana, and Samsung’s Bixby, responded to addiction help-seeking queries. We recorded if IVAs provided a singular response and if so, did they link users to treatment or treatment referral services. Only 4 of the 70 help-seeking queries presented to the five IVAs returned singular responses, with the remainder prompting confusion (e.g., “did I say something wrong?”). When asked “help me quit drugs” Alexa responded with a definition for the word drugs. “Help me quit…smoking” or “tobacco” on Google Assistant returned Dr. QuitNow (a cessation app), while on Siri “help me quit pot” promoted a marijuana retailer. IVAs should be revised to promote free, remote, federally sponsored addiction services, such as SAMSHA’s 1-800-662-HELP helpline. This would benefit millions of IVA users now and more to come as IVAs displace existing information-seeking engines.
R-matrix electron-impact excitation data for the C-like iso-electronic sequence
Authors: Mao J. , Badnell N. R. , Del Zanna G. 
Published: January 2020

Context. Emission and absorption features from C-like ions serve as temperature and density diagnostics of astrophysical plasmas. R-matrix electron-impact excitation data sets for C-like ions in the literature merely cover a few ions, and often only for the ground configuration.
Aims. Our goal is to obtain level-resolved effective collision strength over a wide temperature range for C-like ions from N II to Kr XXXI (i.e., N+ to Kr30+) with a systematic set of R-matrix calculations. We also aim to assess their accuracy.
Methods. For each ion, we included a total of 590 fine-structure levels in both the configuration interaction target and close-coupling collision expansion. These levels arise from 24 configurations 2l3nl′ with n = 2−4, l = 0−1, and l′ = 0−3 plus the three configurations 2s22p5l with l = 0−2. The AUTOSTRUCTURE code was used to calculate the target structure. Additionally, the R-matrix intermediate coupling frame transformation method was used to calculate the collision strengths.
Results. We compare the present results of selected ions with archival databases and results in the literature. The comparison covers energy levels, transition rates, and effective collision strengths. We illustrate the impact of using the present results on an Ar XIII density diagnostic for the solar corona. The electron-impact excitation data is archived according to the Atomic Data and Analysis Structure (ADAS) data class adf04 and will be available in OPEN-ADAS. The data will be incorporated into spectral codes, such as CHIANTI and SPEX, for plasma diagnostics.
Visages de l’enfance aliénée à la fin du XIXe siècle
Authors: Le Bras Anatole 
Published: January 2020

La colonie pour jeunes garçons de l’asile de Vaucluse, créée en 1876, est une institution méconnue et pourtant pionnière de l’investissement du champ de l’enfance par l’aliénisme de la fin du XIXe siècle. L’étude de la population des « colons » montre que l’établissement était un lieu de prise en charge pour des enfants et adolescents atteints de formes de déficience mentale légères et présentant des parcours d’inadaptés, donnant souvent dans la délinquance. Si la colonie n’était pour certains qu’un lieu de passage temporaire, d’autres y entamèrent de longues carrières asilaires, ici abordées à travers deux études de cas de trajectoires de patients.
Endoscopic ultrasound-guided treatment of gastric varices with coil embolization and absorbable hemostatic gelatin sponge: a novel alternative to cyanoacrylate
Authors: Bazarbashi Ahmad Najdat , Wang Thomas J. , Thompson Christopher C. , Ryou Marvin 
Published: January 2020

Abstract
Background and study aims Bleeding from gastric varices (GV) carries high morbidity and mortality. Current endoscopic therapies are premised on cyanoacrylate injection which is technically challenging and carries risk of embolization. We present a case series of endoscopic ultrasound (EUS)-guided coil injection in combination with hemostatic absorbable gelatin sponge (AGS) for treatment of bleeding gastric varices.
Patients and methods This was a retrospective review of EUS-guided coil injection for bleeding GV since November 2017. After EUS-guided needle puncture, hemostatic coils were serially injected until significant reduction of Doppler flow. Under fluoroscopic guidance, test contrast was injected to confirm absence of run-off, at which time AGS, converted into a liquid slurry, was injected as hemostatic reinforcement.
Results Ten consecutive patients underwent EUS-guided coil embolization reinforced by AGS. Technical success, defined as uncomplicated injection of coils and sponge was achieved in 100 % (10/10). Mean follow-up was 6 months 73–397 days; No patients rebled or required reintervention on GV. The complication rate was 10 % (1/10; severe abdominal pain without radiographic findings); otherwise, there were no cases of systemic embolization. Nine of 10 patients (90 %) had follow-up EUS (mean 80 days); 100 % (9/9) revealed near-obliteration of GV.
Conclusion EUS-guided coil embolization in combination with hemostatic AGS is a novel method for management of bleeding GV with high clinical and technical success rates, low risk for complications and favorable safety profile when compared to cyanoacrylate. This technique theoretically minimizes embolic complications and need for re-intervention. Further studies are required to compare this modality.
Prenatal Diagnosis and Outcome of Congenital Corrected Transposition of the Great Arteries – A Multicenter Report of 69 Cases
Authors: Vorisek Carina Nina , Enzensberger Christian , Willomeit Steven , Kurkevych Andrii , Stessig Rüdiger , Ritgen Jochen , Degenhardt Jan , Mielke Gunther , Bosselmann Stephan , Krapp Martin , Slodki Maciej , Respondek-Liberska Maria , Wolter Aline , Kawecki Andrea , Goette Malena , Axt-Fliedner Roland 
Published: January 2020

AbstractObjective Congenital corrected transposition of the great arteries (ccTGA) is a rare congenital cardiac anomaly which remains difficult to diagnose prenatally. We aim to investigate the natural history, associated anomalies and the outcome of patients in prenatally diagnosed ccTGA.Method This was an international multicenter retrospective analysis of fetuses with a diagnosis of ccTGA from 2002 to 2017. We reviewed clinical and echocardiographic databases of seven centers. Anatomic survey and fetal echocardiography were performed according to international guidelines of ISUOG.Results We considered 69 fetuses with prenatally suspected ccTGA. There was an overall survival rate of 91 % among 54 patients with a confirmed diagnosis. Survival to live birth was 96 % (52/54) and survival on an intention-to-treat basis was 94 % (49/52). The mean gestational age at the time of diagnosis was 25.6 ± 5.9 weeks of gestation. In 7 out of 54 fetuses (13 %), ccTGA was an isolated finding. Dextro/mesocardia was present in 15 cases (27.8 %). Intracardiac anomalies were present in 46/54 cases (85.2 %) with the most frequent anomaly being a ventricular septal defect present in 41 fetuses (75.9 %). Complete heart block was diagnosed in 10 cases (18.5 %). Extracardiac anomalies were observed in 9 out of 54 cases (16.7 %). Prenatal karyotyping of the fetus was available in 30/54 (55.6 %) cases with chromosomal anomalies in 4/30 (13.3 %).Conclusion ccTGA is a rare cardiac anomaly often accompanied by a variable spectrum of further intracardiac abnormalities. Accurate diagnosis of ccTGA, which can be integrated into parental counselling, is feasible with a favorable short-term outcome for affected neonates.
Incidence Estimation in Post-ICU Populations: Challenges and Possible Solutions When Using Claims Data
Authors: Brandl Magdalena , Apfelbacher Christian , Weiß Annette , Brandstetter Susanne , Baumeister Sebastian Edgar 
Published: January 2020

Abstract
Background New or worsening cognitive, physical and/or mental health impairments after acute care for critical illness are referred to as “post-intensive care syndrome” (PICS). Little is known about the incidence of its components, since it is challenging to recruit patients after intensive care unit (ICU) treatment for observational studies. Claims data are particularly suited to achieve incidence estimates in difficult-to-recruit groups. However, some limitations remain when using claims data for empirical research on the outcome of ICU treatment. The objective of this article is to describe three challenges and possible solutions for the estimation of the incidence of PICS based on claims data
Methodological challenges: The presence of competing risk by death, investigating a syndrome and dealing with interval censoring First, in (post) ICU populations the assumption of independence between the event of interest (diagnosis of PICS component) and the competing event (death) is violated. Competing risk is an event whose occurrence precludes the event of interest to be observed, and in ICU populations, death is a frequent secondary event. Methods that estimate incidence in the presence of competing risks are well-established but have not been applied to the scenario described above. Second, PICS is a complex syndrome and represented by various ICD-10 (International Classification of Diseases, 10th Revision) disease codes. The operationalization of this syndrome (case identification) and the validation of cases are particularly challenging. Third, another major challenge is that the exact date of the event of interest is not available in claims data. It is only known that the event occurred within a certain interval. This feature is called interval censoring. Recently, methods have been developed that address informative censoring due to competing risks in the presence of interval censoring. We will discuss how these methods could be used to tackle the problem when estimating PICS components. Alternatively, it could be possible to assign an exact date for each diagnosis by combining the diagnosis with the exact date of prescriptions of the respective medicines and/or medical services.
Conclusion Estimating incidence in post-ICU populations entails various methodological issues when using claims data. Investigators need to be aware of the presence of competing risks. The application of internal validation criteria to operationalize the event of interest is crucial to achieve reliable incidence estimates. The problem of interval censoring can be solved either by statistical methods or by combining information from different sources.
Identification of Flavonoids and Bufadienolides and Cytotoxic Effects of Kalanchoe daigremontiana Extracts on Human Cancer Cell Lines
Authors: Stefanowicz-Hajduk Justyna , Asztemborska Monika , Krauze-Baranowska Mirosława , Godlewska Sylwia , Gucwa Magdalena , Moniuszko-Szajwaj Barbara , Stochmal Anna , Ochocka J. Renata 
Published: January 2020

Abstract
Kalanchoe species are well-known medicinal plants used in traditional medicine as anti-inflammatory and analgesic remedies. Recently, it has been reported that Kalanchoe plants have cytotoxic properties; however, data on traditional use of these plants in tumor treatment are extremely limited. Kalanchoe daigremontiana is one of the most popular species cultivated in Europe, and it is used, among other things, as a remedy in treating skin injuries and wounds. Studies on the biological activity of this species are scarce, and there is a lack of data on the cytotoxic activity of K. daigremontiana extracts on epithelial cancer cells in the literature. In our present study, we analyzed the phytochemical composition of K. daigremontiana ethanol extract and fractions–water and dichloromethane–by the HPLC-DAD-ESI-MS method and estimated cytotoxic activity of the extracts on human adenocarcinoma (HeLa), ovarian (SKOV-3), breast (MCF-7), and melanoma (A375) cell lines by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, real-time cell analyzer (RTCA), and flow cytometry. We identified 6 bufadienolide compounds and 19 flavonoids, mostly kaempferol, quercetin, isorhamnetin, and myricetin glycosides, of which only 3 flavonoids have been identified in K. daigremontiana to date. Other flavonoids that were characterized in our study have not yet been found in this plant. The ethanol extract and water fraction of K. daigremontiana did not show significant cytotoxic activity on the tested cell lines. In contrast, the dichloromethane fraction showed the strongest activity against all cell lines with IC50 values of ≤ 10 µg/mL. The results indicated that this activity is mainly due to the presence of bersaldegenin-1,3,5-orthoacetate.