bluuebunny/crossref_metadata_2025_split huggingface dataset:
PSII-40 Effects of combining nose flap weaning with short-term creep feeding of beef calves on cow and calf performance
Authors: Sayre Kendi , Lippolis Katy , Clark Chris 
Published: December 2019

Abstract
One hundred three Angus calves were ranked by gender, BW, age, and dam parity, and assigned to 1 of 4 pre-weaning treatments: 1) nose flaps for 7-d prior to weaning (NF), 2) traditional weaning (TRAD), 3) traditional weaning and creep feed for 3-wk prior to weaning (TRADC), or 4) nose flaps for 7-d prior to weaning and creep feed for 3-wk prior to weaning (NFC). Cow-calf pairs were housed in dry lot pens on d -28. From d -21 to 0, calves in creep treatments were provided free choice access to creep feed. Nose flaps were placed on d -7, and calves were weaned on d 0. Calves were vaccinated and dewormed on d -21 and 0. There was no difference (P ≥ 0.97) in calf BW on d -28 or -21. During the 7-d period that nose flaps were placed, NFC calves had greater (P ≤ 0.0001) ADG than NF and TRAD calves and tended to have greater (P ≤ 0.10) ADG than TRADC calves. At weaning on d 0, TRADC and NFC calves tended to have greater BW (P = 0.07) and had greater overall change in BW (P &lt; 0.0001) during the pre-weaning period than TRAD and NF calves. Additionally, there was a greater (P ≤ 0.001) increase in BW of NFC and TRADC cows during the pre-weaning period compared to NF and TRAD cows. From d -21 to 0 there was no differences (P &gt; 0.41) in plasma concentrations for Bovine Viral Diarrhea Virus (BVD). By d 14, the TRADC calves had the greatest plasma concentrations for BVD (P &lt; 0.04). Therefore, providing short-term creep feed prior to placing nose flaps can improve pre-weaning calf and cow performance compared to traditional and nose flap weaning without creep feed supplementation, however, did not impact response to vaccination.
PSIV-13 Supplemental phytase increases phosphorus digestibility in pigs regardless of phytase source or feed pelleting
Authors: Hong Bokyung , Kim Beob G 
Published: December 2019

Abstract
Two experiments were conducted to investigate the effects of phytase source and feed pelleting on digestibility of dry matter (DM), crude protein, organic matter (OM), and phosphorus (P) with different doses of phytase in pigs. In Exp. 1, ten barrows (31.4 ± 3.5 kg of BW) were individually housed in metabolic crates and allotted to a replicated 5 × 6 Latin square design with 5 diets and 6 periods. In Exp. 2, ten barrows (28.2 ± 3.2 kg of BW) were individually housed in metabolic crates and allotted to a replicated 5 × 5 Latin square design with 5 diets and 5 periods. Each period lasted 10 d consisted of a 5-d adaptation and a 5-d collection periods and the marker to marker method was used for fecal collection. In both experiments, a basal diet was prepared based on corn and soybean meal without phytase or inorganic P. Four additional diets were prepared in a 2 × 2 factorial treatment arrangement with 2 phytase sources (Buttiauxella spp. or Escherichia coli) and 2 feed forms (mash or pellet). Both experiments employed the same treatments but had different supplemental phytase doses (500 and 1,500 FTU/kg in Exp. 1 and 2, respectively). In Exp. 1, supplemental phytase increased standardized total tract digestibility of P (66.1 vs. 51.7%; P &lt; 0.001) with no effects of phytase source or feed pelleting. Feed pelleting increased digestibility of DM, crude protein, and OM (P &lt; 0.001). In Exp. 2, supplemental phytase increased standardized total tract digestibility of P (72.8 vs. 44.8%; P &lt; 0.001) with no effects of phytase source or feed pelleting. Feed pelleting increased digestibility of DM and OM (P &lt; 0.001). Taken together, supplemental phytase at 500 and 1,500 FTU/kg increases P digestibility regardless of phytase source or feed pelleting.
PSII-25 The effect of the organic acid on the bulls’ sperm quality
Authors: Anipchenko Polina , Plemyashov Kirill , Nikitin Georgiy , Nikitina Anastasia , Shabunin Sergey 
Published: December 2019

Abstract
Carnitine is actively used in medicine in the treatment of male infertility. L-carnitine is an organic acid that normalizes the balance of oxidative and antioxidant processes. Bulls (n = 15) with low sperm quality (low sperm activity) were selected. The bulls were injected intramuscularly L-carnitine solution (NaturVet, Australia) at the rate of 1 ml per 30 kg of animal body weight twice a week for one month. A macroscopic assessment of the ejaculate was carried out, the activity of the spermatozoa in the freshly obtained ejaculate, the concentration, the activity of the spermatozoa after thawing the sperm samples was evaluated.Investigated samples obtained from bulls one month before the start of L-carnitine using, during the period of the experiment and during one month after the end of the experiment.The minimum value of sperm activity in the period prior to the use of L-carnitine was 2 points. The average activity of bull semen is 2.7 points. Research results showed that the average score of sperm activity increased to 7.2 points. The maximum value of sperm activity is 8 points.The volume in the period prior to the injection of L-carnitine was 1.9 ml, while the drug was in use - 3.4 ml. The sperm production rate increased by 44%.The average value of sperm concentration in the ejaculate of bulls when using L- carnitine was 0.9 billion / ml. This indicator increased by 11% compared to this indicator in the period before using of the drug (0.8 billion / ml). Thus, based on the data obtained, it can be noted that using the injection form of the L-carnitine solution has a positive effect on the quality of sperm obtained from bulls.
PSII-28 Impact of commingling on feedlot steer productivity
Authors: Hubbard Amanda J , Sawyer Jason , Cooke Reinaldo F , Daigle Courtney L 
Published: December 2019

Abstract
This study evaluated the impact of commingling two sources of cattle on productivity during the feedlot phase of production. Angus crossbred steers (n = 96), similar in genetic composition and age, from two research stations (n = 48 McG and n = 48 BCS) were transported (833.64 ± 85.29 km) to a feedlot 39-d prior to study commencement. Steers were blocked by source and stratified by d -34 body weight (BW) before being randomly assigned to one of 12 pens (n = 8 steers/pen) for the 42-d study. Pens were assigned to one of two treatments (n = 6 pens/treatment): 1) NOMIX—100% of cattle from either McG (n = 3 pens) or 100% of cattle from BCS (n = 3 pens) and, 2) MIX—50% of cattle from BCS and 50% from McG (n = 6 pens). Body weight was collected on d0, on d24, and at the end of the study on d42. Orthogonal contrasts analyzed effects of commingling (NOMIX vs. MIX) and source (BCS vs. McG) on dry matter intake (DMI), average daily gain (ADG), and gain to feed (G:F). Linear mixed models (PROC MIXED) evaluated impact of source × treatment on ADG. No effects of commingling (P &gt; 0.05) or source (P &gt; 0.05) were detected on DMI (MIX 8.25, BCS 8.70, McG 8.14 kg/steer/day), ADG (MIX: 1.48, BCS: 1.67, McG: 1.59 kg/steer), and G:F (MIX: 0.18, BCS: 0.19, McG: 0.19). There was no effect of source × treatment on ADG (P &gt; 0.05); however, McG-MIX had the smallest ADG (1.42 kg/steer) compared to BCS-MIX, BCS-NOMIX, and McG-MIX (1.61, 1.59, and 1.60 kg/steer, respectively). Source and mixing treatment did not statistically impact productivity or efficiency, however, mixed cattle were numerically less productive than non-mixed cattle, and the impact of mixing on productivity may vary by source.
PSI-35 Comparison of in situ techniques to evaluate the recovery of indigestible components and the accuracy of digestibility estimates
Authors: Adams Jordan , Norris Aaron B , Tedeschi Luis O 
Published: December 2019

Abstract
Our objective was to evaluate the effects of bag type (BT) and incubation length (IL) when using in situ incubations for determination of indigestible components. Indigestible components play an integral role as an internal marker for rumen kinetics and digestibility estimations, as well as providing parameter estimates for nutritional models. However, the accuracy of internal markers is dependent upon the incubation technique utilized, with BT and IL acting as sources of error. A 3 × 2 factorial design was used to investigate the effect of BT (F57, F58, and Dracon) and IL (288 and 576 h) on indigestible dry matter (iDM) and neutral detergent fiber (iNDF) residues, as well as estimated DM and NDF digestibilities. Feed and fecal samples were obtained from two animals from an independent trial that collected total fecal production over 4 days. All bags contained a sample size-to-surface area ratio of 20 mg/cm2 and were ruminally incubated within three rumen-cannulated steers (229.5 ± 10 kg). For iDM and iNDF, feed residues demonstrated a BT × IL interaction (P &lt; 0.01). Fecal residues for iDM and iNDF had BT and IL effects (P &lt; 0.01) with the Dracon BT and 576 h IL having the least residues. Digestibility estimates for DM and NDF were influenced by BT × IL (P &lt; 0.01) with the accuracy of estimates demonstrating a BT × IL interaction (P &lt; 0.01) in which F57 at 576 h had the greatest accuracy. Resultant fecal recovery estimates for DM and NDF excreted exhibited a BT × IL interaction (P &lt; 0.01), with F57 at 576 h better representing the excretion profiles. Based upon our analysis, utilizing the F57 BT at 576 h IL combination provides more accurate estimates relative to all other combinations used within the current study.
PSVIII-31 Genome-wide estimation of linkage disequilibrium using American mink genotyping-by-sequencing data
Authors: Karimi Karim , Farid A Hossain , Sargolzaei Mehdi , Myles Sean , Miar Younes 
Published: December 2019

Abstract
Linkage disequilibrium (LD) has been defined as the correlation between alleles at different loci in the genome. The LD levels can be influenced by the evolutionary processes and historical events in populations. The main objective of this study was to estimate the LD levels at different distances of American mink genome using genotyping-by-sequencing (GBS) data. A total of 285 American mink (Neovison vison) were sequenced based on GBS libraries prepared by digesting the genomic DNA with the restriction enzyme ApeKI. After quality control, 13,321 single nucleotide polymorphism (SNP) markers located on 46 Scaffolds were used to determine the extension of LD in the genome. The average r2 was computed for all syntenic SNP pairwise at inter-marker distances from 0 up to 1 Mb. The average r2 between adjacent SNPs was 0.29, ranged from 0.18 to 0.53 across all scaffolds. In addition, the average distance between adjacent markers was 51 kb. The average r2 above 0.3 was observed in less than 1 kb distances and declined with increase in distances between markers. The average r2 was estimated to be less than 0.2 for markers more than 10 kb apart. Furthermore, the average LD level was decreased to 0.08 for inter-marker distances between 0.9 and 1 Mb. The results of this study can be used to determine the optimum maker density required for obtaining enough accuracy and power in both genomic selection and genome-wide association studies.
PSVIII-24 Inbreeding levels of the Line 4 Hereford cattle population
Authors: Hieber Jordan , Thomson Jennifer 
Published: December 2019

Abstract
Inbreeding is an increasing issue in the beef cattle industry due to increased use of artificial insemination (AI) and embryo transfer (ET). Inbreeding, or increased relatedness between animals, results in inbreeding depression and its effects have been well documented; reduced performance, reproduction, and profitability. However, there is a lack of understanding the molecular mechanisms involved in inbreeding depression. Long-term linebred populations offer a unique opportunity to better understand this, more specifically the Line 4 Hereford population. The population was established from the Line 1 Hereford population in 1962 and has been maintained by the Montana State University (MSU) Northern Agricultural Research Center (NARC) near Havre, MT since its establishment. Inbreeding was estimated using a complete pedigree (FPED) and genomic information on a subset of the population. A pedigree containing 3,453 animals was constructed covering years 1962 – 2018 and was used to calculate FCPED. Animals were selected for genotyping based on genetic contributions and availability. 241 semen, tissue, and blood samples were collected and genotyped with the Illumina Bovine GGP 50K BeadChip. Genomic inbreeding (FG) and pedigree inbreeding (FGPED) were evaluated for the 241 genotyped animals. Average rate of change in inbreeding per year was also evaluated. Runs of Homozygosity (ROH) analysis was performed in Golden Helix SVS v8.8.3. ROH were defined as a minimum run length of 500 kb with a minimum of 20 SNP. Inbreeding ranges were 0 – 34%, 0 – 98%, and 0 – 27% and the average inbreeding was 10.0%, 11.4%, and 15.3% for FPED, FG, and FGPED, respectively. The average rate of change in inbreeding per year was 0.3% over 57 years. Initial analysis found 30 regions identified by ROH, indicating that we can use ROH analysis and potentially Genome-Wide Association Studies (GWAS) to identify regions of the genome being impacted by inbreeding depression.
PSIII-35 Effects of dietary macronutrient profile on apparent total tract macronutrient digestibility and fecal metabolites of female dogs after spay surgery
Authors: Phungviwatnikul Thunyaporn , Valentine Helen , He Fei , Swanson Kelly S 
Published: December 2019

Abstract
Obesity reduces the quality and length of life of companion animals. Spaying increases risk, but dietary modification may attenuate obesity and related co-morbidities. The objective of this study was to determine the effects of specially formulated diets on apparent total tract macronutrient digestibility (ATTD) and fecal metabolites of female dogs after spay surgery. All procedures were approved by the University of Illinois Institutional Animal Care and Use Committee prior to experimentation. Twenty-four dogs were spayed and randomly allotted to three experimental diets: 1) moderate-protein, moderate-fiber diet (control; COSP), 2) high-protein, high-fiber diet (HP-HF), or 3) high-protein, high-fiber diet plus omega-3 fats (HP-HF-O). Four dogs were sham-operated and fed the control diet (COSH). Dogs were fed to maintain BW for 12 weeks after spay, then allowed to consume twice that amount for another 12 weeks. Change from baseline data were analyzed statistically, with P &lt; 0.05 being significantly different. Food intake in dogs fed HP-HF or HP-HF-O was greater than COSH dogs, but COSP dogs were intermediate. Fat and organic matter digestibilities were lower in dogs fed HP-HF or HP-HF-O compared with COSH and COSP dogs. Fecal dry matter percentage and volume increases were greater in HP-HF and HP-HF-O dogs than COSH and COSP dogs. HP-HF and HP-HF-O dogs had higher fecal acetate than COSH or COSP dogs. Fecal butyrate tended to be greater (P = 0.08) in HP-HF dogs than COSH dogs. COSH and HP-HF-O dogs had lower fecal valerate than COSP dogs, while fecal ammonia tended to be higher (P = 0.05) in HP-HF-O than COSP and HP-HF dogs. Fecal total short-chain fatty acid (SCFA) increases in HP-HF-O dogs were higher than COSH dogs. Our results indicate that a high-protein, high-fiber diet can reduce energy content by reducing fat digestibility without affecting protein digestibility, modulate fecal SCFA concentrations, and decrease fecal branched-chain fatty acid.
PSVIII-12 Comparative characterization of intestinal alkaline phosphatase kinetics in young piglets and human Caco-2 cells
Authors: Burello Nicole , Zhang Hua , Wang Weijun , Archbold Tania , Tsao Rong , Fan Ming Z 
Published: December 2019

Abstract
This study reports the kinetics of young porcine jejunal alkaline phosphatase (IAP) towards the dephosphorylation of ATP, lipopolysaccharides (LPS) and p-nitrophenyl phosphate (pNPP) at the physiological conditions (pH = 7.4; and at 37 °C) in comparison with the IAP from human Caco-2 cells. The 10-day suckling young porcine jejunal IAP displayed the Km values of 1.26±0.50 mM, 1.35±0.64 mg/mL and 0.290±0.072 mM for the hydrolyses of ATP, LPS and pNPP, respectively; while the respective Km values were 0.030±0.007 mM, 0.66±0.22 mg/mL and 0.033±0.006 mM for the IAP in the Caco-2 cells towards the same set of substrates. In comparison, the Km values of the young porcine jejunal IAP were 2–40 times higher than those of IAP from the human Caco-2 cells. In addition, Pearson correlation analyses showed tight positive correlations (P &lt; 0.001) between IAP activities towards pNPP, ATP and LPS in the piglet and the Caco-2 cells, suggesting that the IAP activity towards pNPP could be used to predict the IAP activities on the physiological substrates of ATP and LPS. Lastly, four AP genes were identified in the genome of Sus scrofa. Three of these porcine AP genes are annotated as intestinal-type alkaline phosphatase (IAP) genes and clustered at the distal end of chromosome 15, namely IAPX1, IAPX2, and IAPX3. The genomic context of APs in the pig genome is highly similar to those in the human genome. We predict that pig IAPX3 (XP_003133777.1) is likely an IAP gene for pigs. Further comparisons in post-translational modification and protein 3-D structure modelling were performed, indicating that the observed differences in kinetic affinity between the young porcine jejunal IAP and the human Caco-2 cell IAP in hydrolyzing ATP, LPS and pNPP might relate to their differences in the coding sequences of the IAP protein, and/or the IAP protein N-glycosylation.
PSI-5 Intestinal responses and the determination of true total tract trace mineral digestibility and the endogenous losses in weanling pigs by the regression analysis technique
Authors: Yang Zeyu , Wu Hongzhi , Archbold Tania , Yin Xindi , Fan Wenyi 
Published: December 2019

Abstract
There is limited knowledge regarding trace mineral bioavailability in trace mineral supplements and common feed ingredients and trace mineral endogenous losses in pigs. The objectives of this study were to investigate intestinal responses and to determine true total tract trace mineral digestibility and the endogenous losses of trace minerals associated with corn and SBM based diets in weanling pigs by the regression analysis. Twenty-four crossbred barrows, with an average initial BW of 14 kg, were randomly assigned to 4 weanling pig diets with inclusion of titanium dioxide (0.30%) and a commercial trace mineral-vitamin premix at 0.125, 0.250, 0.375 and 0.500%, respectively, to result in 4 graded dietary levels of Cu, Zn, Fe, Mn and Se. The pigs were fed close to ad libitum for 11 d according to a randomized complete block design. The dietary inclusion did not affect (P &gt; 0.05) growth performances, ileal and fecal DM digestibility, D-mannitol gut permeability and jejunal alkaline phosphatase kinetics. With the regression analysis, true total tract Cu, Zn, Fe, Mn and Se digestibility values (±SE; n = 23 or 24; P &lt; 0.05) in the trace mineral-vitamin premix were determined to be 137.3±27.9, 40.5±15.4, 66.2±24.6, 94.3±21.6 and 96.6±2.8% vs. the corresponding values in the four weanling pig diets for Cu at 137.4±3.2, Zn at 40.4±3.1, Fe at 66.2±3.0, Mn at 94.2±3.8and Se at 96.2±0.8%, respectively. The total tract endogenous Cu, Zn, Fe, Mn and Se outputs (±SE)associated with the weanling pig diets were also determined (23.4±0.6, 9.2±2.7, 37.2±3.5, 19.4±1.0 and 0.07±0.003 mg/kg DMI diets; n = 23 or 24;P &lt; 0.05). Our results have shown that the intestinal physiological functions and performances were not significantly affected in the weanling pigs fed on the diets with reduced gradient levels of a trace mineral-vitamin premix. Total tract true digestible trace mineral supply should be considered in swine diet formulation.
PSXIV-32 Oleic acid stimulate the formation of adipocyte-like cells from bovine satellite cells via G Protein Coupled Receptor 43 and CCAAT/Enhancer Binding Protein Beta
Authors: Kim Jongkyoo , Chung Ki Yong , Johnson Bradley J , Smith Stephen B 
Published: December 2019

Abstract
Numerous physiological and pathological processes are controlled by free fatty acids (FFAs), which act as a signaling molecule in mammals. We hypothesized that oleic acid (Ole) may stimulate the formation of satellite cell-derived intramuscular adipose tissue. The objective of the current study was to determine the effect of Ole on GPR43 and factors related to the adipogenic differentiation of bovine satellite cells. Bovine satellite cells were isolated from the semimembranosus of two 14-month-old crossbreed steers. The isolated muscle satellite cells were incubated in Dulbecco’s Modified Eagle’s Medium (DMEM) solution with 10% Fetal Bovine Serum. Upon reaching 80 to 90% confluence, the growth medium was replaced with differentiation medium composed of DMEM and 2% horse serum, 10μg/mL insulin, 10μg/mL hydrocortisone, 10μM ciglitizone, and 1×antibiotic-antimycotic with dose of: 0, 1, 10, 100, or 500 μM of oleic acid (Ole). Addition of Ole on BSC induced transdifferentiation of myogenic lineage into adipocyte-like cells which formed lipid droplets within cells. Use of 100 μM and 500 μM Ole doses tended to result in a greater (P &lt; 0.1) amount of mRNA gene expression of C/EBPβ compared to all other doses. This might suppress myogenic differentiation. Expression of PPARγ was not altered (P &gt; 0.1) by treatment. The addition of 100 μM and 500 μM upregulated (P &lt; 0.05) mRNA gene expression of GPR43 and 100 μM of Ole increased protein level of GPR43 (P &lt; 0.05) and phosphorylated AMPKα (P &lt; 0.05).
PSVII-37 First molecular detection of Equine Herpesvirus type 2 (EHV-2) and type 5 (EHV-5) in upper respiratory liquids of healthy training horses from southern Brazil
Authors: Biava Janaina S , Finger Mariane Angélica , Ullmann Leila , Biondo Alexander , Leutenegger Christian , Filho Ivan Barros 
Published: December 2019

Abstract
Multiple pathogens may cause and sustain respiratory infection in horses, either alone or as co-infection, leading to disruption and interruption of training, racing and other competitions. Molecular approaches have successfully provided opportunity for pathogen involvement and characterization, particularly from samples of horse upper respiratory tract. Accordingly, the aim of this study was to molecularly assess bronchoalveolar lavage fluid (BALF) samples from 10 healthy training horses at the Jockey Club of Parana State, southern Brazil. Virus-specific real-time polymerase chain reaction (qPCR) assays were applied as panel for concomitant detection of Equine Adenovirus, Equine Influenza Virus, Equine Herpesvirus Type 1 (EHV-1), Type 2 (EHV-2), Type 4 (EHV-4), and Type 5 (EHV-5), Equine Rhinitis A Virus, Equine Rhinitis B Virus, and Streptococcus equi subsp. equi. Amplicons from positive samples were amplified and sequenced to confirm the virus identification. Results have confirmed the first molecular detection of EHV-2 and EHV-5 in healthy training horses of Brazil, with an overall 3/10 (30%) EHV positivity. Two horses were positive for at least one EHV, and one was positive for both EHV-2 and EHV-5. No horse presented clinical signs of respiratory disease at the time or following samplings. These findings may provide new insights on the epidemiological situation of EHV-2 and EHV-5 in Brazil, highlighting the importance of molecular detection and active surveillance in clinically healthy horses, which may act as spreading infection source for younger equines.