bluuebunny/crossref_metadata_2025_split huggingface dataset:
Characterisation and outcome of neuropsychiatric symptoms in patients with anti-NMDAR encephalitis
Authors: Restrepo-Martinez Miguel , Ramirez-Bermudez Jesus , Bayliss Leo , Espinola-Nadurille Mariana 
Published: November 2019

AbstractBackground:Encephalitis due to anti-N-methyl-D-aspartate receptor antibodies (ANMDARE) is the most frequent immune-mediated encephalitis. It is distinguished by the subacute onset of neuropsychiatric symptoms.Objective:To evaluate the characteristic neuropsychiatric symptoms and their outcome in patients diagnosed with ANMDARE.Methods:This was a prospective, longitudinal study in patients with a diagnostic suspicion of ANMDARE that presented to the National Institute of Neurology from March 2018 to February 2019. A comparative analysis of two groups (positive N-methyl-D-aspartate receptor [NMDAR] vs. negative NMDAR antibodies in cerebrospinal fluid [CSF]) was done on admission and at discharge. Neuropsychiatric systematic assessments included the Neuropsychiatric Inventory Questionnaire, the Bush Francis Catatonia Rating Scale, the Confusion Assessment Method Severity, the Montreal Cognitive Assessment, and the Overt Agitation Severity Scale.Results:24 individuals were analysed: 14 had positive NMDAR antibodies, and 10 had negative NMDAR antibodies in CSF. On admission, agitation/aggression, euphoria/exaltation, and disinhibition were more common in patients with positive antibodies. Excited catatonia and delirium were diagnosed more frequently in patients with positive antibodies. At discharge, there was an important decrease in neuropsychiatric symptoms, but substantial cognitive impairment remained. The mean hospitalisation length was 41.71 (SD 39.33) days for patients with definitive ANMDARE (p 0.259).Conclusions:Neuropsychiatric symptoms profile in ANMDARE was associated with the early onset of euphoria/exaltation and disinhibition, accompanied by marked psychomotor agitation. When ANMDARE was suspected, the presence of excited-type catatonia and delirium showed a tendency to predict definitive ANMDARE. At discharged, most patients recovered from catatonia, delirium, and psychosis, but marked cognitive symptoms, anxiety, and depression persisted at discharge.
In major depression, increased kappa and mu opioid receptor levels are associated with immune activation
Authors: Al-Hakeim Hussein Kadhem , Zeki Al-Fadhel Suhaer , Al-Dujaili Arafat Hussein , Maes Michael 
Published: November 2019

AbstractObjective:This study was carried out to delineate differences between major depressive disorder (MDD) and healthy controls in dynorphin and kappa opioid receptor (KOR) levels in association with changes in the β-endorphin – mu opioid receptor (MOR) and immune-inflammatory system.Methods:The present study examines dynorphin, KOR, β-endorphin, MOR, interleukin (IL)-6 and IL-10 in 60 drug-free male participants with MDD and 30 age-matched healthy males.Results:Serum dynorphin, KOR, β-endorphin and MOR are significantly higher in MDD as compared to controls. The increases in the dynorphin/KOR system and β-endorphin/MOR system are significantly intercorrelated and are both strongly associated with increased IL-6 and IL-10 levels. Dynorphin, β-endorphin, KOR and both cytokines showed a good diagnostic performance for MDD versus controls with a bootstrapped (n = 2000) area under the receiver operating curve of 0.972. The dynorphin/KOR system is significantly decreased in depression with comorbid nicotine dependence.Conclusion:Our findings suggest that, in MDD, immune activation is associated with a simultaneous activation of dynorphin/KOR and β-endorphin/MOR signaling and that these opioid systems may participate in the pathophysiology of depression by (a) exerting immune-regulatory activities attenuating the primary immune response and (b) modulating reward responses and mood as well as emotional and behavioural responses to stress.
CONFLICTING EVIDENCE OF EXPLICIT AND IMPLICIT KNOWLEDGE FROM OBJECTIVE AND SUBJECTIVE MEASURES
Authors: Maie Ryo , DeKeyser Robert M. 
Published: November 2019

AbstractThis study is the first to compare objective and subjective measures of explicit and implicit knowledge under learning from incidental exposure. An experiment was conducted, during which L1 English speakers were trained on a semiartificial language, Japlish. A measure of explicit knowledge and a recently proposed measure of implicit knowledge (i.e., an untimed auditory grammaticality judgment and a word-monitoring task) were applied to gauge the two types of knowledge at two testing sessions, and their results were compared with those of subjective measures of awareness. Results revealed clear discrepancies between the two measurement approaches in terms of their sensitivity. In particular, while the subjective measures varied in identifying explicit and implicit knowledge of various Japlish constructions, the objective measures indicated that most of the knowledge was explicit, and development of implicit knowledge (measured by the word-monitoring task) was minimal, only manifested in detecting a case-missing violation at the delayed posttest. The results are discussed with reference to the current literature on explicit and implicit learning and knowledge, and it is concluded that the criterion of (un)awareness might not be by itself sufficient to provide a full account of L2 knowledge developed under incidental conditions.
The Eocene Protohierodula crabbi Ross, 2019 cannot be reliably assigned to Manteidae (Insecta: Mantodea): A reply
Authors: ROSS Andrew J. 
Published: November 2019

ABSTRACTI accept Schubnel & Nel's (2019) opinion that Protohierodula belongs to the clade Artimantodea, that it cannot be reliably assigned to the family Manteidae and should be regarded as family incertae sedis.
Rapid Stage‐Discharge Rating Curve Assessment Using Hydraulic Modeling in an Uncertainty Framework
Authors: Mansanarez Valentin , Westerberg Ida K. , Lam Norris , Lyon Steve W. 
Published: November 2019

AbstractEstablishing reliable streamflow time series is essential for hydrological studies and water‐related decisions, but it can be both time‐consuming and costly since streamflow is typically calculated from water level using rating curves based on numerous calibration measurements (gaugings). It can take many years of gauging data collection to estimate reliable rating curves, and even then extreme‐flow estimates often still depend on rating curve extrapolation. Hydraulically modeled rating curves are a promising alternative to traditional methods as they can be rapidly derived with few concurrent stage‐discharge gaugings. We introduce a novel framework for Rating curve Uncertainty estimation using Hydraulic Modelling (RUHM), based on Bayesian inference and physically based hydraulic modeling for estimating stage‐discharge rating curves and their associated uncertainty. The framework incorporates information from the river shape, hydraulic configuration, and the control gaugings as well as uncertainties in the gaugings and model parameters. We explored the interaction of uncertainty sources within RUHM by (1) assessing its performance at two Swedish stations, (2) investigating the sensitivity of the results to the number and magnitude of the calibration gaugings, and (3) evaluating the importance of prior information on the model parameters. We found that rating curves with constrained uncertainty could be estimated using only three gaugings for either low or low and medium flows that have a high probability of occurrence, thereby enabling rapid rating curve estimation. Prior information about the water‐surface slope‐stage relation, obtainable from site surveys, was needed to adequately constrain uncertainty estimates.
A Subcritical Water Extractor Prototype for Potential Astrobiology Spaceflight Missions
Authors: Kehl F. , Kovarik N. A. , Creamer J. S. , da Costa E. T. , Willis P. A. 
Published: November 2019

AbstractA top‐level NASA exploration goal is the search for signs of life beyond Earth. Molecular biosignatures can be sought via in situ organic chemical analyses of samples collected from planetary bodies. Current spaceflight‐ready technologies lack the required sensitivity to perform these analyses on key polar organic species primarily due to the low efficiency of transferring these molecules from natural samples into chemical instrumentation. One promising approach to improve the liberation of these molecules from sample matrices prior to analysis is through the use of liquid extraction, in which organic molecules are dissolved in heated, pressurized water. This process has never been performed on a spaceflight mission. As part of instrument maturation efforts, we describe the development of the first spaceflight prototype of a fully automated subcritical water extractor designed to provide this essential functionality on a potential future planetary mission. The prototype extractor was mounted on a Mars test rover platform and successfully operated remotely in the Atacama Desert, Chile. Samples acquired by the rover's drill and sample acquisition/delivery system were remotely transferred to the extractor inlet funnel, and all subsequent extractor operations were performed automatically. To validate the instrument and demonstrate its suitability as a front‐end unit for an organic analyzer, we tested low‐bioload Atacama Desert soil extracts for amino acid content using capillary electrophoresis coupled to laser‐induced fluorescence. We show that hot extraction under subcritical conditions is required to liberate amino acids from the sample, as no amino acids were found in the extract produced at room temperature.
Measuring the Elemental Composition of Phobos: The Mars‐moon Exploration with GAmma rays and NEutrons (MEGANE) Investigation for the Martian Moons eXploration (MMX) Mission
Authors: Lawrence David J. , Peplowski Patrick N. , Beck Andrew W. , Burks Morgan T. , Chabot Nancy L. , Cully Michael J. , Elphic Richard C. , Ernst Carolyn M. , Fix Samuel , Goldsten John O. , Hoffer Erin M. , Kusano Hiroki , Murchie Scott L. , Schratz Brian C. , Usui Tomohiro , Yokley Zachary W. 
Published: November 2019

AbstractThe Mars‐moon Exploration with GAmma rays and NEutrons (MEGANE) investigation will use gamma‐ray and neutron spectroscopy to measure the elemental composition of Mars' moon Phobos. MEGANE is part of the Japanese Martian Moons eXploration (MMX) mission that will make comprehensive remote sensing measurements of both of Mars' moons Phobos and Deimos. MMX will also return to Earth regolith samples of Phobos. The science goals of the MEGANE investigation mirror those of the MMX mission. MEGANE will use elemental composition measurements to determine if Phobos is a captured asteroid or the end result of a giant impact event on Mars, study Phobos surface processes, provide reconnaissance to support the sample site selection, and supply compositional context for the returned samples. To accomplish its measurements, MEGANE will use a high‐purity Ge gamma‐ray spectrometer (GRS), and a neutron spectrometer (NS) that consists of two3He gas proportional neutron sensors. The GRS derives heritage from similar instruments from NASA's MErcury Surface, Space ENvironment, GEochemistry, and Ranging mission and the Psyche mission that is currently in development; the NS is based on similar instruments used for NASA's Lunar Prospector and Psyche missions.
Impact of Special Collections in JGR Space Physics
Authors: Liemohn Michael W. , Wooden Paige R. 
Published: November 2019

AbstractJournals occasionally solicit manuscripts for special collections, in which all papers are focused on a particular topic within the journal's scope. For the Journal of Geophysical Research: Space Physics, there have been 51 special collections from 2005 through 2018, with a total of 1,009 papers out of the 8,881 total papers in the journal over those years (11%). Taken together, the citations to special collection papers, as well as other metrics, are slightly higher than papers not in special collections. Several paper characteristics were examined to assess whether they could explain the higher citation and download values for special collection papers, but they cannot. In addition, indirect methods were conducted for assessing self‐citations as an explanation for the increased citations, but no evidence was found to support this hypothesis. It was found that some paper types, notably Commentaries and Technical Reports, have lower average citations but higher average downloads than Research Articles (the most common type of paper in this journal). This implies that such paper types have a different kind of impact than “regular” science‐result‐focused papers. In addition to having higher average citations and downloads, special collections focus community attention on that particular research topic, providing a deadline for manuscript submissions and a single webpage at which many related papers are listed. It is concluded that special collections are worth the extra community effort in organization, writing, and reviewing these papers.
Self‐Organizing Maps for Evaluation of Biogeochemical Processes and Temporal Variations in Water Quality of Subtropical Reservoirs
Authors: Melo Darllene S. , Gontijo Erik S. J. , Frascareli Daniele , Simonetti Vanessa C. , Machado Leila S. , Barth Johannes A. C. , Moschini‐Carlos Viviane , Pompêo Marcelo L. , Rosa André H. , Friese Kurt 
Published: November 2019

AbstractWater quality in reservoirs is often compromised in many regions worldwide by nutrients and trace metals. This demands continuous monitoring; however, analyses of large data sets collected during regular monitoring remain a difficult task. Multivariate techniques offer a fast and robust approach for interpreting complex results. The objective of this study was to check the efficacy of self‐organizing maps (SOMs) as a tool to investigate biogeochemical processes. This tool can also help to illustrate influences of land use patterns on the water quality of reservoirs. Here we use the Itupararanga Reservoir in Brazil as a subtropical example. Vertical profiles were sampled from seven sites in the reservoir in a total of seven campaigns over 24 months. Next to physicochemical parameters in the water column (dissolved oxygen, Eh, pH, and temperature), levels of nutrients (NO3−, NH4+, and PO43−), transition and trace metals (Al, Ba, Ca, Cr, Cu, Fe, K, Mg, Mn, Na, Ni, and Zn), and chlorophyll‐a (Chla) were measured. These variables were correlated with land use using SOM. With this technique samples were classified into 17 distinct groups that showed distinct influences of spatial heterogeneity and seasonality. The analyses helped to reveal a seasonal stratification period, where Fe, Mn, and P were released from sediments. Nutrients and some metal inputs (Al and Fe) were related to agricultural, urban, and grass/pasture areas around the reservoir. Our approach also helped to explain physical and biogeochemical seasonality in the reservoir.
Biochemical sensing in graphene-enhanced microfiber resonators with individual molecule sensitivity and selectivity
Authors: Cao Zhongxu , Yao Baicheng , Qin Chenye , Yang Run , Guo Yanhong , Zhang Yufeng , Wu Yu , Bi Lei , Chen Yuanfu , Xie Zhenda , Peng Gangding , Huang Shu-Wei , Wong Chee Wei , Rao Yunjiang 
Published: November 2019

AbstractPhotonic sensors that are able to detect and track biochemical molecules offer powerful tools for information acquisition in applications ranging from environmental analysis to medical diagnosis. The ultimate aim of biochemical sensing is to achieve both quantitative sensitivity and selectivity. As atomically thick films with remarkable optoelectronic tunability, graphene and its derived materials have shown unique potential as a chemically tunable platform for sensing, thus enabling significant performance enhancement, versatile functionalization and flexible device integration. Here, we demonstrate a partially reduced graphene oxide (prGO) inner-coated and fiber-calibrated Fabry-Perot dye resonator for biochemical detection. Versatile functionalization in the prGO film enables the intracavity fluorescent resonance energy transfer (FRET) to be chemically selective in the visible band. Moreover, by measuring the intermode interference via noise canceled beat notes and locked-in heterodyne detection with Hz-level precision, we achieved individual molecule sensitivity for dopamine, nicotine and single-strand DNA detection. This work combines atomic-layer nanoscience and high-resolution optoelectronics, providing a way toward high-performance biochemical sensors and systems.
A brainstem-central amygdala circuit underlies defensive responses to learned threats
Authors: Gu Yiran , Piper Walter T. , Branigan Lauren A. , Vazey Elena M. , Aston-Jones Gary , Lin Longnian , LeDoux Joseph E. , Sears Robert M. 
Published: November 2019

AbstractNorepinephrine (NE) plays a central role in the acquisition of aversive learning via actions in the lateral nucleus of the amygdala (LA) [1, 2]. However, the function of NE in expression of aversively-conditioned responses has not been established. Given the role of the central nucleus of the amygdala (CeA) in the expression of such behaviors [3–5], and the presence of NE axons projections in this brain nucleus [6], we assessed the effects of NE activity in the CeA on behavioral expression using receptor-specific pharmacology and cell- and projection-specific chemogenetic manipulations. We found that inhibition and activation of locus coeruleus (LC) neurons decreases and increases freezing to aversively conditioned cues, respectively. We then show that locally inhibiting or activating LC terminals in CeA is sufficient to achieve this bidirectional modulation of defensive reactions. These findings support the hypothesis that LC projections to CeA are critical for the expression of defensive responses elicited by conditioned threats.
Clinical significance and biological role of L1 cell adhesion molecule in gastric cancer
Authors: Ichikawa Takashi , Okugawa Yoshinaga , Toiyama Yuji , Tanaka Koji , Yin Chengzeng , Kitajima Takahito , Kondo Satoru , Shimura Tadanobu , Ohi Masaki , Araki Toshimitsu , Kusunoki Masato 
Published: November 2019

Abstract
Background
L1 cell adhesion molecule (L1CAM) is highly expressed in malignant tumours and might play a pivotal role in tumour progression.

Methods
We analysed by immunohistochemistry L1CAM protein expression in formalin-fixed, paraffin-embedded specimens from 309 GC patients. We performed propensity score matching (PSM) analysis to clarify the prognostic impact of L1CAM in GC patients. We evaluated L1CAM gene expression in fresh frozen specimens from another group of 131 GC patients to establish its clinical relevance. The effects of changes in L1CAM were investigated in vitro and in vivo.

Results
L1CAM was mainly expressed in tumour cells of GC tissues. Elevated L1CAM expression was an independent prognostic factor for overall and disease-free survival, and an independent risk factor for distant metastasis in GC patients. PSM analysis showed that high L1CAM expression was significantly associated with poor prognosis. L1CAM gene expression using fresh frozen specimens successfully validated all of these findings in an independent cohort. Inhibition of L1CAM suppressed cell proliferation, cycle progress, invasion, migration and anoikis resistance in GC cells. Furthermore, L1CAM inhibition suppressed the growth of peritoneal metastasis.

Conclusion
L1CAM may serve as a feasible biomarker for identification of patients who have a high risk of recurrence of GC.
Evidence that cyanobacterial Sll1217 functions analogously to PGRL1 in enhancing PGR5-dependent cyclic electron flow
Authors: Dann Marcel , Leister Dario 
Published: November 2019

AbstractIn plants and cyanobacteria, the PGR5 protein contributes to cyclic electron flow around photosystem I. In plants, PGR5 interacts with PGRL1 during cyclic electron flow, but cyanobacteria appear to lack PGRL1 proteins. We have heterologously expressed the PGR5 and PGRL1 proteins from the plant Arabidopsis in various genetic backgrounds in the cyanobacterium Synechocystis. Our results show that plant PGR5 suffices to re-establish cyanobacterial cyclic electron flow (CEF), albeit less efficiently than the cyanobacterial PGR5 or the plant PGR5 and PGRL1 proteins together. A mutation that inactivates Arabidopsis PGR5 destabilises the protein in Synechocystis. Furthermore, the Synechocystis protein Sll1217, which exhibits weak sequence similarity with PGRL1, physically interacts with both plant and cyanobacterial PGR5 proteins, and stimulates CEF in Synechocystis. Therefore, Sll1217 partially acts as a PGRL1 analogue, the mode of action of PGR5 and PGRL1/Sll1217 proteins is similar in cyanobacteria and plants, and PGRL1 could have evolved from a cyanobacterial ancestor.
Structure of the native supercoiled flagellar hook as a universal joint
Authors: Kato Takayuki , Makino Fumiaki , Miyata Tomoko , Horváth Péter , Namba Keiichi 
Published: November 2019

AbstractThe Bacterial flagellar hook is a short supercoiled tubular structure made from a helical assembly of the hook protein FlgE. The hook acts as a universal joint that connects the flagellar basal body and filament, and smoothly transmits torque generated by the rotary motor to the helical filament propeller. In peritrichously flagellated bacteria, the hook allows the filaments to form a bundle behind the cell for swimming, and for the bundle to fall apart for tumbling. Here we report a native supercoiled hook structure at 3.6 Å resolution by cryoEM single particle image analysis of the polyhook. The atomic model built into the three-dimensional (3D) density map reveals the changes in subunit conformation and intersubunit interactions that occur upon compression and extension of the 11 protofilaments during their smoke ring-like rotation. These observations reveal how the hook functions as a dynamic molecular universal joint with high bending flexibility and twisting rigidity.
Synergy between Cyclase-associated protein and Cofilin accelerates actin filament depolymerization by two orders of magnitude
Authors: Shekhar Shashank , Chung Johnson , Kondev Jane , Gelles Jeff , Goode Bruce L. 
Published: November 2019

AbstractCellular actin networks can be rapidly disassembled and remodeled in a few seconds, yet in vitro actin filaments depolymerize slowly over minutes. The cellular mechanisms enabling actin to depolymerize this fast have so far remained obscure. Using microfluidics-assisted TIRF, we show that Cyclase-associated protein (CAP) and Cofilin synergize to processively depolymerize actin filament pointed ends at a rate 330-fold faster than spontaneous depolymerization. Single molecule imaging further reveals that hexameric CAP molecules interact with the pointed ends of Cofilin-decorated filaments for several seconds at a time, removing approximately 100 actin subunits per binding event. These findings establish a paradigm, in which a filament end-binding protein and a side-binding protein work in concert to control actin dynamics, and help explain how rapid actin network depolymerization is achieved in cells.