bluuebunny/crossref_metadata_2025_split huggingface dataset:
ATM and PRDM9 regulate SPO11-bound recombination intermediates during meiosis
Authors: Paiano Jacob , Wu Wei , Yamada Shintaro , Sciascia Nicholas , Callen Elsa , Paola Cotrim Ana , Deshpande Rajashree A. , Maman Yaakov , Day Amanda , Paull Tanya T. , Nussenzweig André 
Published: February 2020

AbstractMeiotic recombination is initiated by SPO11-induced double-strand breaks (DSBs). In most mammals, the methyltransferase PRDM9 guides SPO11 targeting, and the ATM kinase controls meiotic DSB numbers. Following MRE11 nuclease removal of SPO11, the DSB is resected and loaded with DMC1 filaments for homolog invasion. Here, we demonstrate the direct detection of meiotic DSBs and resection using END-seq on mouse spermatocytes with low sample input. We find that DMC1 limits both minimum and maximum resection lengths, whereas 53BP1, BRCA1 and EXO1 play surprisingly minimal roles. Through enzymatic modifications to END-seq, we identify a SPO11-bound meiotic recombination intermediate (SPO11-RI) present at all hotspots. We propose that SPO11-RI forms because chromatin-bound PRDM9 asymmetrically blocks MRE11 from releasing SPO11. In Atm–/– spermatocytes, trapped SPO11 cleavage complexes accumulate due to defective MRE11 initiation of resection. Thus, in addition to governing SPO11 breakage, ATM and PRDM9 are critical local regulators of mammalian SPO11 processing.
Outer membrane protein size and LPS O-antigen define protective antibody targeting to the Salmonella surface
Authors: Domínguez-Medina C. Coral , Pérez-Toledo Marisol , Schager Anna E. , Marshall Jennifer L. , Cook Charlotte N. , Bobat Saeeda , Hwang Hyea , Chun Byeong Jae , Logan Erin , Bryant Jack A. , Channell Will M. , Morris Faye C. , Jossi Sian E. , Alshayea Areej , Rossiter Amanda E. , Barrow Paul A. , Horsnell William G. , MacLennan Calman A. , Henderson Ian R. , Lakey Jeremy H. , Gumbart James C. , López-Macías Constantino , Bavro Vassiliy N. , Cunningham Adam F. 
Published: February 2020

AbstractLipopolysaccharide (LPS) O-antigen (O-Ag) is known to limit antibody binding to surface antigens, although the relationship between antibody, O-Ag and other outer-membrane antigens is poorly understood. Here we report, immunization with the trimeric porin OmpD from Salmonella Typhimurium (STmOmpD) protects against infection. Atomistic molecular dynamics simulations indicate this is because OmpD trimers generate footprints within the O-Ag layer sufficiently sized for a single IgG Fab to access. While STmOmpD differs from its orthologue in S. Enteritidis (SEn) by a single amino-acid residue, immunization with STmOmpD confers minimal protection to SEn. This is due to the OmpD-O-Ag interplay restricting IgG binding, with the pairing of OmpD with its native O-Ag being essential for optimal protection after immunization. Thus, both the chemical and physical structure of O-Ag are key for the presentation of specific epitopes within proteinaceous surface-antigens. This enhances combinatorial antigenic diversity in Gram-negative bacteria, while reducing associated fitness costs.
Mode-phase-difference photothermal spectroscopy for gas detection with an anti-resonant hollow-core optical fiber
Authors: Zhao Pengcheng , Zhao Yan , Bao Haihong , Ho Hoi Lut , Jin Wei , Fan Shangchun , Gao Shoufei , Wang Yingying , Wang Pu 
Published: February 2020

AbstractLaser spectroscopy outperforms electrochemical and semiconductor gas sensors in selectivity and environmental survivability. However, the performance of the state-of-the-art laser sensors is still insufficient for many high precision applications. Here, we report mode-phase-difference photothermal spectroscopy with a dual-mode anti-resonant hollow-core optical fiber and demonstrate all-fiber gas (acetylene) detection down to ppt (parts-per-trillion) and <1% instability over a period of 3 hours. An anti-resonant hollow-core fiber could be designed to transmit light signals over a broad wavelength range from visible to infrared, covering molecular absorption lines of many important gases. This would enable multi-component gas detection with a single sensing element and pave the way for ultra-precision gas sensing for medical, environmental and industrial applications.
Metabolic responses of adult lion’s paw scallops Nodipecten subnodosus exposed to acute hyperthermia in relation to seasonal reproductive effort
Authors: Salgado-García Rosa L. , Kraffe Edouard , Maytorena-Verdugo Claudia I. , Rivera-Camacho Alma R. , Sicard M. Teresa , Arellano-Martínez Marcial , Racotta Ilie S. 
Published: February 2020

AbstractIn marine ectotherms, reproduction is an energetically expensive process that affects their thermal window tolerance. For most species, the impacts of hyperthermia during gametogenesis have still not been addressed. Our aim was to assess the metabolic response of adult Nodipecten subnodosus scallops to thermal challenges at early development (spring) and advanced gonad maturation (summer). Scallops collected in both seasons were exposed to acute hyperthermia (26 and 30 °C, 24 h), maintaining a group of scallops at acclimation temperature (22 °C) as a control condition. During the summer, relatively low activity of hexokinase (HK), as well as low levels of ATP and GTP were found in the adductor muscle, suggesting a shift in energy investment for reproduction, although arginine phosphate (ArgP) levels were higher in summer scallops. Hyperthermia (30 °C) induced an increased energy expenditure reflected by a transitory enhanced oxygen consumption (VO2) and relatively high activities of HK and arginine kinase (AK). Moreover, a slight decrease in adenylic energy charge (AEC) was partially compensated by a decrease in ArgP. An increase in nucleotide by-products inosine monophosphate (IMP) and hypoxanthine (HX) indicated a thermal stress at 30 °C. Some of the responses to acute hyperthermia were more pronounced at advanced maturation stages (summer scallops), indicating a possible lack of energy balance, with possible implications in animals challenged to global warming scenario.
Fully Printed PEDOT:PSS-based Temperature Sensor with High Humidity Stability for Wireless Healthcare Monitoring
Authors: Wang Yi-Fei , Sekine Tomohito , Takeda Yasunori , Yokosawa Koji , Matsui Hiroyuki , Kumaki Daisuke , Shiba Takeo , Nishikawa Takao , Tokito Shizuo 
Published: February 2020

AbstractFacile fabrication and high ambient stability are strongly desired for the practical application of temperautre sensor in real-time wearable healthcare. Herein, a fully printed flexible temperature sensor based on cross-linked poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) (PEDOT:PSS) was developed. By introducing the crosslinker of (3-glycidyloxypropyl)trimethoxysilane (GOPS) and the fluorinated polymer passivation (CYTOP), significant enhancements in humidity stability and temperature sensitivity of PEDOT:PSS based film were achieved. The prepared sensor exhibited excellent stability in environmental humidity ranged from 30% RH to 80% RH, and high sensitivity of −0.77% °C−1for temperature sensing between 25 °C and 50 °C. Moreover, a wireless temperature sensing platform was obtained by integrating the printed sensor to a printed flexible hybrid circuit, which performed a stable real-time healthcare monitoring.
Gene expression in mouse muscle over time after nickel pellet implantation
Authors: Bannon Desmond I , Bao Wenjun , Turner Stephen D , McCain Wilfred C , Dennis William , Wolfinger Russ , Perkins Ed , Abounader Roger 
Published: February 2020

Abstract
The transition metal nickel is used in a wide variety of alloys and medical devices. Nickel can cause a range of toxicities from allergy in humans to tumors when implanted in animals. Several microarray studies have examined nickel toxicity, but so far none have comprehensively profiled expression over an extended period. In this work, male mice were implanted with a single nickel pellet in the muscle of the right leg with the left leg used as a control. At 3 week intervals up to 12 months, nickel concentrations in bioflulids and microarrays of surrounding tissue were used to track gene expression patterns. Pellet biocorrosion resulted in varying levels of systemic nickel over time, with peaks of 600 μg L−1 in serum, while global gene expression was cyclical in nature with immune related genes topping the list of overexpressed genes. IPA and KEGG pathway analyses was used to attribute overall biological function to changes in gene expression levels, supported by GO enrichment analysis. IPA pathways identified sirtuin, mitochondria, and oxidative phosphorylation as top pathways, based predominantly on downregulated genes, whereas immune processes were associated with upregulated genes. Top KEGG pathways identified were lysosome, osteoclast differentiation, and phasgosome. Both pathway approaches identified common immune responses, as well as hypoxia, toll like receptor, and matrix metalloproteinases. Overall, pathway analysis identified a negative impact on energy metabolism, and a positive impact on immune function, in particular the acute phase response. Inside the cell the impacts were on mitochondria and lysosome. New pathways and genes responsive to nickel were identified from the large dataset in this study which represents the first long-term analysis of the effects of chronic nickel exposure on global gene expression.
„Old Friends“, Immunregulation und Stressresilienz
Authors: Langgartner Dominik , Lowry Christopher A. , Reber Stefan O. 
Published: February 2020

ZUSAMMENFASSUNGEs gibt zahlreiche Hinweise darauf, dass chronische negative Erfahrungen, insbesondere chronischer psychosozialer Stress, einen wesentlichen Risikofaktor für die Entstehung vieler somatischer und affektiver Störungen darstellen, einschließlich chronischer entzündlicher Darmerkrankungen (CED) und der posttraumatischen Belastungsstörung (PTBS). Die Mechanismen, die der Entstehung chronischer stressbedingter Erkrankungen zugrunde liegen, sind jedoch noch weitgehend unbekannt und die derzeitigen Behandlungs- und Präventionsstrategien sind unzureichend wirksam und zuverlässig. Ein besseres Verständnis der Mechanismen, die an der Entstehung und Aufrechterhaltung chronischer stressbedingter Erkrankungen beteiligt sind, kann zu neuen Ansätzen in der Prävention und Behandlung dieser Erkrankungen führen. In diesem 2-teiligen Review fassen wir die theoretischen Grundlagen (Teil 1) sowie die zugrunde liegenden Mechanismen (Teil 2) zusammen, welche die Hypothese stützen, dass ein Anstieg der Immun(re)aktivität und des Entzündungsgeschehens in der heutigen modernen Gesellschaft, potenziell gefördert durch einen verminderten Kontakt mit immunregulatorischen Mikroorganismen („Old Friends“), ursächlich an der gesteigerten Anfälligkeit für stressbedingte Pathologien sein könnte. Darüber hinaus diskutieren wir den künstlich herbeigeführten Kontakt mit Old Friends als vielversprechende Strategie zur Förderung der Stressresilienz und zur Vorbeugung/Behandlung chronischer stressbedingter Erkrankungen.
Rolle des Mikrobioms und der Darm-Gehirn-Interaktion bei Anorexia nervosa
Authors: Trinh Stefanie , Herpertz-Dahlmann Beate , Seitz Jochen 
Published: February 2020

ZUSAMMENFASSUNGDas menschliche Darm-Mikrobiom ist am Stoffwechsel, der Gewichtsregulierung, der Entwicklung des Immunsystems sowie an der Interaktion zwischen Darm und Gehirn des Wirtes beteiligt. Patientinnen mit Anorexia nervosa (AN) zeigen deutliche Mikrobiomveränderungen, zum Beispiel eine reduzierte Bakterienvielfalt. AN-bezogene Veränderungen im Mikrobiom können die Darmpermeabilität, Entzündungsprozesse und die Bildung von Autoantikörpern fördern. Eine reduzierte Bakterienvielfalt in AN scheint mit Depressions-, Angst- und Essstörungssymptomen zusammenzuhängen. Auf das Mikrobiom ausgerichtete Therapien wie Ernährungsinterventionen oder Prä- und Probiotika könnten zusätzliche Behandlungsperspektiven bieten.
A Case Presentation on Rheumatic Heart Disease with Mitral Regurgitation
Authors: A. Jayasudha , B. Sreerenjini , P. Kaveri , P. Anitha 
Published: February 2020

AbstractDuring any pregnancy there is an increase in blood volume of 30 to 50% resulting in increased pressure on the heart valves. For women with rheumatic heart disease this increased pressure presents increased maternal and/or fetal risks. Counseling of women with rheumatic heart disease gives appropriate surveillance of maternal and fetal well-being, as well as planning and documentation of the management of elective and emergency delivery. The care of pregnant women with rheumatic heart disease thus requires a multidisciplinary approach, involving obstetricians, cardiologists, and anesthetists. However, many women with rheumatic heart disease have healthy pregnancies and healthy babies with the right medical care.
To Share is Human! Advancing Evidence into Practice through a National Repository of Interoperable Clinical Decision Support
Authors: Lomotan Edwin A. , Meadows Ginny , Michaels Maria , Michel Jeremy J. , Miller Kristen 
Published: February 2020

Abstract
Background Healthcare systems devote substantial resources to the development of clinical decision support (CDS) largely independently. The process of translating evidence-based practice into useful and effective CDS may be more efficient and less duplicative if healthcare systems shared knowledge about the translation, including workflow considerations, key assumptions made during the translation process, and technical details.
Objective Describe how a national repository of CDS can serve as a public resource for healthcare systems, academic researchers, and informaticists seeking to share and reuse CDS knowledge resources or “artifacts.”
Methods In 2016, the Agency for Healthcare Research and Quality (AHRQ) launched CDS Connect as a public, web-based platform for authoring and sharing CDS knowledge artifacts. Researchers evaluated early use and impact of the platform by collecting user experiences of AHRQ-sponsored and community-led dissemination efforts and through quantitative/qualitative analysis of site metrics. Efforts are ongoing to quantify efficiencies gained by healthcare systems that leverage shared, interoperable CDS artifacts rather than developing similar CDS de novo and in isolation.
Results Federal agencies, academic institutions, and others have contributed over 50 entries to CDS Connect for sharing and dissemination. Analysis indicates shareable CDS resources reduce team sizes and the number of tasks and time required to design, develop, and deploy CDS. However, the platform needs further optimization to address sociotechnical challenges. Benefits of sharing include inspiring others to undertake similar CDS projects, identifying external collaborators, and improving CDS artifacts as a result of feedback. Organizations are adapting content available through the platform for continued research, innovation, and local implementations.
Conclusion CDS Connect has provided a functional platform where CDS developers are actively sharing their work. CDS sharing may lead to improved implementation efficiency through numerous pathways, and further research is ongoing to quantify efficiencies gained.
Theoretical and experimental investigation of Lamb waves excited by partially debonded rectangular piezoelectric transducers
Authors: Shpak A N , Mueller I , Golub M V , Fritzen C P 
Published: February 2020

Abstract
The paper proposes a new hybrid approach technique to simulate acousto-ultrasonic wave excitation and propagation due to operation of the partially debonded piezoelectric transducer attached to a plate-like structure. The semi-analytical boundary integral equation method is applied to calculate guided waves propagation in the unbounded structures and to separate different guided waves in the piezo-induced wave-fields. The obtained model is verified experimentally using the scanning laser Doppler vibrometry. Eigenfrequencies are calculated and analysed for various sizes of the transducer and for different bonding conditions between the transducer and the waveguide. The impact of the transducer’s height, size and debonding area on symmetric and antisymmetric Lamb waves excitation is analysed. The paper demonstrates that one-sided debonding of the transducer exerts intense influence on the distribution of the wave energy among the excited Lamb wave modes, while center debonding has a sizable impact only at relatively high frequencies.
Many-body quantum dynamics and induced correlations of Bose polarons
Authors: Mistakidis S I , Koutentakis G M , Katsimiga G C , Busch Th , Schmelcher P 
Published: February 2020

Abstract
We study the ground state properties and non-equilibrium dynamics of two spinor bosonic impurities immersed in a one-dimensional bosonic gas upon applying an interspecies interaction quench. For the ground state of two non-interacting impurities we reveal signatures of attractive induced interactions in both cases of attractive or repulsive interspecies interactions, while a weak impurity–impurity repulsion forces the impurities to stay apart. Turning to the quench dynamics we inspect the time-evolution of the contrast unveiling the existence, dynamical deformation and the orthogonality catastrophe of Bose polarons. We find that for an increasing postquench repulsion the impurities reside in a superposition of two distinct two-body configurations while at strong repulsions their corresponding two-body correlation patterns show a spatially delocalized behavior evincing the involvement of higher excited states. For attractive interspecies couplings, the impurities exhibit a tendency to localize at the origin and remarkably for strong attractions they experience a mutual attraction on the two-body level that is imprinted as a density hump on the bosonic bath.
Transverse quasi-modes in periodic potentials
Authors: Alberucci Alessandro 
Published: February 2020

Abstract
We discuss how to find the quasi-modes of the Schrödinger equation when the potential is periodic in time. Our method confirms that the profile of the time-independent (continuous) component of the wavefunction obeys an effective Schrödinger equation where the potential is given by the Kapitza term plus the temporal mean of the original potential, a result originally found by Rahav et al (2003 Phys. Rev. Lett. 91 110404). We then find closed-form expression for the higher order corrections to the quasi-modes, showing how the generic quasi-mode undergoes periodic temporal oscillations and a non-flat phase profile. Validity of our theoretical results is verified against full numerical simulations of the Schrödinger equation. Our findings can be applied both to quantum mechanics and light propagation in the paraxial regime.
Unveiling the oxidation behavior of liquid-phase exfoliated antimony nanosheets
Authors: Assebban Mhamed , Gibaja Carlos , Fickert Michael , Torres Iñigo , Weinreich Erik , Wolff Stefan , Gillen Roland , Maultzsch Janina , Varela Maria , Tan Jun Rong Sherman , Loh Kian Ping , Michel Enrique G , Zamora Félix , Abellán Gonzalo 
Published: February 2020

Abstract
Antimonene, a monolayer of β-antimony, is increasingly attracting considerable attention, more than that of other monoelemental two-dimensional materials, due to its intriguing physical and chemical properties. Under ambient conditions, antimonene exhibits a high thermodynamic stability and good structural integrity. Some theoretical calculations predicted that antimonene would have a high oxidation tendency. However, it remains poorly investigated from the experimental point of view. In this work, we study the oxidation behavior of antimonene nanosheets (ANS) prepared by ultrasonication-assisted liquid-phase exfoliation. Using a set of forefront analytical techniques, a clear effect of sonication time on the surface chemistry of prepared ANS is found. A dynamic oxidation behavior has been observed, which upon annealing at moderate temperature (210 °C) resulted in a semiconducting behavior with a bandgap of approximately 1 eV measured by ultraviolet photoelectron spectroscopy. This study yields valuable information for future applications of antimonene and paves the way towards novel modification approaches in order to tailor its properties and complement its limitations.
Effect of different surface conditions on fatigue properties of 7N01 aluminum alloy and the behavioral mechanism of crack of the alloy under alternating load
Authors: Chen Zhiyuan , Cai Zhengkun , Deng Peng , Jiang Xiaosong , Chen Song , Sun Hongliang 
Published: February 2020

Abstract
In this paper, the surface of 7N01 aluminum alloy profile is sandblasted or electropolished. The influence of different surface conditions on its conditional fatigue strength and fatigue mechanism under the alternating load after prefabricated short crack are studied. Results show that the 7N01 aluminum alloy with sandblasting treatment has formed a work hardened layer and its fatigue strength is increased by 42.9% comparing with the original surface specimens. The fatigue strength of the 7N01 aluminum alloy after electropolishing is almost unchanged compared with the original specimen. The fatigue behavior of the 7N01 aluminum alloy after prefabricated side straight through the notch deviated from the theoretical smooth specimen law and long crack propagation law in which is a ‘shortage effect’. When the notch size is smaller than the transition crack size a0, the fatigue limit of the notched specimen is lower than the fatigue limit of the smooth specimen. When the notch size is larger than the transition crack size, the fatigue limit of the long-notched specimen is still lower than that predicted by linear elastic fracture mechanics. Due to the ‘short notch effect’, the effective notch propagation threshold ΔKeff.th of the long crack is 0.39 Mpa m1/2 based on the effective notch propagation size a0 eff of the alloy.
A guide to household manual and machine dishwashing through a life cycle perspective
Authors: Porras Gabriela Y , Keoleian Gregory A , Lewis Geoffrey M , Seeba Nagapooja 
Published: February 2020

Abstract
This study evaluates and provides guidance on improving the life cycle environmental performance of dishwashing in the typical U.S. household. Typical user behaviors and recommended best practices for manual dishwashing as well as machine dishwasher use are evaluated. A sensitivity analysis shows the influence of varying grid carbon intensity, water heater type, regional water scarcity, and behaviors such as pre-rinsing and machine loading on overall results. Use-phase behaviors are observed through a small-scale laboratory study. Dishwashing following typical manual and machine practices produces 5,620 and 2,090 kg CO2e life cycle greenhouse gas (GHG) emissions respectively based on washing 4 loads (8 place settings per load) a week for 10 years. Avoiding typical behaviors like pre-rinsing and selecting heated dry can decrease life cycle GHG emissions for machine dishwashing by 3% and 11%, respectively. The running tap style of manual dishwashing results in the highest life cycle GHG emissions of the alternatives in the lab study. Manual dishwashing has the potential to have the lowest GHG emmisions (1,610 kg) when recommended behaviors are followed, less than the 1,960 kg CO2e for recommended machine dishwasher use. When life cycle water consumption burdens are evaluated, typical manual and machine dishwashing use 34,200 and 16,300 gallons respectively and these results are contextualized to regions with different water scarcity. A life cycle cost (LCC) analysis finds that machine dishwashing costs more than manual dishwashing over a 10-year lifetime even if best practices are followed. However, when a user’s time spent washing is valued, machine dishwashers pay for themselves within a year of use.