bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hsp90 and its co-chaperone Sti1 control TDP-43 misfolding and toxicity
Authors: Lin Lilian Tsai-Wei , Razzaq Abdul , Di Gregorio Sonja E. , Hong Soojie , Charles Brendan , Lopes Marilene H. , Beraldo Flavio , Prado Vania F. , Prado Marco A.M. , Duennwald Martin L. 
Published: October 2020

AbstractProtein misfolding is a central feature of most neurodegenerative diseases. Molecular chaperones can modulate the toxicity associated with protein misfolding, but it remains elusive which molecular chaperones and co-chaperones interact with specific misfolded proteins. TDP-43 misfolding and inclusion formation is a hallmark of amyotrophic lateral sclerosis (ALS) and other neurodegenerative diseases. Using yeast and mammalian neuronal cells we find that Hsp90 and its co-chaperones have a strong capacity to alter TDP-43 misfolding, inclusion formation, aggregation, and cellular toxicity. Our data also demonstrate that impaired Hsp90 function sensitizes cells to TDP-43 toxicity. We further show that the co-chaperone Sti1 specifically interacts with and modulates TDP-43 toxicity in a dose-dependent manner. Our study thus uncovers a previously unrecognized tie between Hsp90, Sti1, TDP-43 misfolding, and its cellular toxicity.
The transcriptome of regenerating zebrafish scales identifies genes involved in human bone disease
Authors: Bergen Dylan J.M. , Tong Qiao , Shukla Ankit , Newman Elis , Zethof Jan , Lundberg Mischa , Ryan Rebecca , Youlten Scott E. , Zeggini Eleftheria , Croucher Peter I. , Flik Gert , Richardson Rebecca J. , Kemp John P. , Hammond Chrissy L. , Metz Juriaan R. 
Published: October 2020

AbstractZebrafish scales are mineralised plates that can regenerate involvingde novobone formation. This presents an opportunity to uncover genes and pathways relevant to human musculoskeletal disease relevant to impaired bone formation. To investigate this hypothesis, we defined transcriptomic profiles of ontogenetic and regenerating scales, and identified 604 differentially expressed genes (DEGs) that were enriched for extracellular matrix, ossification, and cell adhesion pathways. Next, we showed that human orthologues of DEGs were 2.8 times more likely to cause human monogenic skeletal diseases (P<8×10−11), and they showed enrichment for human orthologues associated with polygenetic disease traits including stature, bone density and osteoarthritis (P<0.005). Finally, zebrafish mutants of two human orthologues that were robustly associated with height and osteoarthritis (COL11A2) or bone density only (SPP1) developed skeletal abnormalities consistent with our genetic association studies.Col11a2Y228X/Y228Xmutants showed endoskeletal features consistent with abnormal growth and osteoarthritis, whereasspp1P160X/P160Xmutants had elevated bone density (P<0.05). In summary, we show that transcriptomic studies of regenerating zebrafish scales have potential to identify new genes and pathways relevant to human skeletal disease.
SARS-CoV-2 elicits robust adaptive immune responses regardless of disease severity
Authors: Nielsen Stine SF , Vibholm Line K , Monrad Ida , Olesen Rikke , Frattari Giacomo S , Pahus Marie H , Højen Jesper F , Gunst Jesper D , Erikstrup Christian , Holleufer Andreas , Hartmann Rune , Østergaard Lars , Søgaard Ole S , Schleimann Mariane H , Tolstrup Martin 
Published: October 2020

AbstractThe SARS-CoV-2 pandemic currently prevails worldwide. To understand the immunological signature of SARS-CoV-2 infections and aid the search for treatments and vaccines, comprehensive characterization of adaptive immune responses towards SARS-CoV-2 is needed. We investigated the breadth and potency of antibody-, and T-cell immune responses, in 203 recovered SARS-CoV-2 infected patients who presented with asymptomatic to severe infections. We report very broad serological profiles with cross-reactivity to other human coronaviruses. Further, >99% had SARS-CoV-2 epitope specific antibodies, with SARS-CoV-2 neutralization and spike-ACE2 receptor interaction blocking observed in 95% of individuals. A significant positive correlation between spike-ACE2 blocking antibody titers and neutralization potency was observed. SARS-CoV-2 specific CD8+T-cell responses were clear and quantifiable in 90% of HLA-A2+individuals. The viral surface spike protein was identified as the dominant target for both neutralizing antibodies and CD8+T cell responses. Overall, the majority of patients had robust adaptive immune responses, regardless of disease severity.Author summarySARS-CoV-2 can cause severe and deadly infections. However, the immunological understanding of this viral infection is limited. Currently, several vaccines are being developed to help limit transmission and prevent the current pandemic. However, basic understanding of the adaptive immune response developed during SARS-CoV-2 infections is needed to inform further vaccine development and to understand the protective properties of the developed immune response. We investigated, the adaptive immune response developed during SARS-CoV-2 infections in recovered patients experiencing a full spectrum of disease severity, from asymptomatic infections to severe cases requiring hospitalization. We used a novel multiplex serological platform, cell-based neutralization assays and dextramer flow cytometry assays to characterize a broad and robust humoral and cellular immune response towards SARS-CoV-2. We found that the vast majority of recovered individuals have clear detectable and functional SARS-CoV-2 spike specific adaptive immune responses, despite diverse disease severities. The detection of both a humoral and cellular functional spike specific immune response in the vast majority of the individuals, irrespective of asymptomatic manifestations, supports vaccine designs currently underway, and encourages further exploration of whether primary infections provide protection to reinfection.
A deep learning framework for elucidating whole-genome chemical interaction space
Authors: Cai Tian , Lim Hansaim , Abbu Kyra Alyssa , Qiu Yue , Nussinov Ruth , Xie Lei 
Published: October 2020

AbstractMolecular interaction is the foundation of biological process. Elucidation of genome-wide binding partners of a biomolecule will address many questions in biomedicine. However, ligands of a vast number of proteins remain elusive. Existing methods mostly fail when the protein of interest is dissimilar from those with known functions or structures. We develop a new deep learning framework DISAE that incorporates biological knowledge into self-supervised learning techniques for predicting ligands of novel unannotated proteins on a genome-scale. In the rigorous benchmark studies, DISAE outperforms state-of-the-art methods by a significant margin. The interpretability analysis of DISAE suggests that it learns biologically meaningful information. We further use DISAE to assign ligands to human orphan G-Protein Coupled Receptors (GPCRs) and to cluster the human GPCRome by integrating their phylogenetic and ligand relationships. The promising results of DISAE open an avenue for exploring the chemical landscape of entire sequenced genomes.
Development and training for the dealers' community in Macau casinos
Authors: Zhou Jinquan 
Published: October 2020

PurposeThe purpose of this paper is to discuss characteristics of dealers training and development in Macau gambling industry by investigating and analyzing the current dealers training and development status of Macau gambling casinos. Training and development prepare and enhance dealer's knowledge and skills to enable them to adapt to the working environment and create a career opportunity in their organization.Design/methodology/approachIn-depth interviews were conducted with Macau casino human recourse manger, and questionnaire survey was used to investigate the views of dealers in training and development provided by their organization.FindingsThis paper revealed that the development and training of Macao's dealers are mainly in individual professional skills, including executive ability, professional attitude and communication skills training. According to the current situation of dealer's training and development, a human resource investment policy is that the government requires undertaking a part of the work in the dealers' community.Research limitations/implicationsThis paper examined the current demand for talents in Macau gambling casinos focusing on dealers' survey of the casino's training concepts, including training perception, organization arrangement and training information, etc. It is the least published official information, and training and development program in Macau gambling industry wreaked the questionnaire design.Practical implicationsThis survey first time revealed a characteristic of dealer's human resources training and development in the Macau gambling industry. The authors got comprehensive and profound understanding of dealers' job skills and career development needs through the professional position and job characteristics investigation and analysis.Social implicationsThe investigation reveals the inadequately training offered for dealers pre-employment. At least, it cannot meet the gambling industry's basic needs for dealer occupation in many ways. The school's training of dealers and the training provided by the casino has a huge gap, which the school's training is a short-term, compared to the gambling enterprises on the professional skills requirements of recruit dealers. But the school in long-term provide a wider range of knowledge for dealers career development.Originality/valueThis research conducted by the author in 2017 showed that dealer training and development views could be used to in the gambling industry in human resource practical management.
A novel PV array interconnection scheme to extract maximum power based on global shade dispersion using grey wolf optimization algorithm under partial shading conditions
Authors: R. Balraj , Stonier Albert Alexander 
Published: October 2020

Purpose
Partial shading causes significant power decreases in the PV systems. The purpose of this paper is to address this problem, connectivity regulation is designed to reduce partial shading problems.

Design/methodology/approach
In this approach, the partial shading was estimated and dispersed evenly on the whole array by global shade dispersion technique (GSD). The grey wolf algorithm was implemented for the interconnection of arrays by an efficient switching matrix.

Findings
After the implementation of the GSD technique using a grey wolf algorithm, the performance under different shading conditions was analyzed using the MatLab simulation tool. The results were compared with total cross-tied (TCT), Su Do Ku and the proposed method of reconfiguration, where the proposed method improves the maximum power of the PV system appropriately.

Research limitations/implications
This methodology uses any size of PV systems.

Social implications
Replacement of conventional energy systems with renewable energy systems such as solar helps the environment clean and green.

Originality/value
The GSD interconnection scheme using the grey wolf optimization algorithm has proved an improved output performance compared with the existing TCT and Sudoku based reconfiguration techniques. By comparing with existing techniques in literature, the proposed method is more advantageous for reducing mismatch losses between the modules of any size of the PV array with less operating time.
The unwitting corruption of broadening of marketing into neoliberalism: a beast unleashed?
Authors: Dholakia Nikhilesh , Ozgun Aras , Atik Deniz 
Published: October 2020

PurposeThis paper aims to uncover links, overlaps and influence flows across two seemingly unrelated historical processes – the broadening of the marketing concept and the rapid rise of neoliberal ideology, and associated economic and social policies.Design/methodology/approachHistorical examination of the pivotal points in marketing thought, especially since 1960s and 1970s, is juxtaposed with the historical rise of neoliberalism to uncover linkages between marketing and neoliberalism, with a particular reference to Foucault’s analysis of the neoliberal transgression of classical liberalism.FindingsWhile noble intentions were behind the broadening of the concept of marketing, the implicit assumptions reinforced neoliberal ideology and policies that led to rapid rise in inequality and to disastrous financial and economic crises.Research limitations/implicationsThis study, relying on extensive interdisciplinary theorizing, could benefit from empirical and practical extensions.Practical implicationsGlobally pervasive marketing practices – based on the broadening of the marketing concept – have become imbricated in contemporary spiraling crises. To escape such spirals, radical rethinking of marketing theories and practices is required.Social implicationsTo reorient away from serving only the interests of centralized capital and to serve the needs of people the world over, marketing thought and practice need to reorient to innovative ideas that transcend the broadened and generic marketing concepts.Originality/valueThe paper develops the linkages between marketing theory and practices since the late 1960s and the neoliberal ideology politics and policies, with roots in the 1920s, that rose to prominence in the 1970s. A key contribution is an exploration of, in a marketing context, Foucault’s analysis of the neoliberal eclipsing of classical liberalism.
Empirical relationship between workers' remittances and financial development (an ARDL cointegration approach for Sri Lanka)
Authors: Mohamed Aslam Ahamed Lebbe , Sivarajasingham Selliah 
Published: October 2020

PurposeThe purpose of this study aims to investigate the nature of the relationship between workers' remittances and financial development (FD) in Sri Lanka for the period from 1975 to 2017.Design/methodology/approachThis study used both the exploratory data analysis and inferential data analysis (IDA) techniques to test the objective of this study. The IDA technique consisted of the augmented Dickey–Fuller (ADF) and Phillips–Perron unit root tests, the autoregressive distributed lag (ARDL) bounds cointegration technique, the Granger causality test and impulse response function analysis.FindingsThe unit root test results show that the variables are in mixed order. The empirical results of cointegration confirm that workers' remittances have a beneficial long-run relationship with FD in Sri Lanka. The Granger causality test result indicates that there is a bidirectional relationship between workers' remittances and FD. The impulse response analysis indicates that a positive shock to workers' remittance has an immediate significant positive impact on the FD of up to 10 years.Practical implicationsThe analytical techniques used in this study explain how workers' remittances induce FD in Sri Lanka.Originality/valueThis study fills an important gap in the academic literature by using newly developed ARDL bounds cointegration techniques in Sri Lanka, by using impulse response function analysis, and by studying the dynamic relationship between workers' remittances and FD using time series data.
What is the effect of banking concentration and competition on financial development? An international assessment
Authors: De Moraes Claudio Oliveira , Antunes José Americo Pereira , Coutinho Márcio Silva 
Published: October 2020

PurposeThis paper analyzes the effect of the banking market (concentration and competition) on financial development.Design/methodology/approachIn order to estimate the effects of banking concentration and competition on financial development, we conducted an empirical analysis using the System Generalized Method of Moments (S-GMM) through a dynamic panel data model.FindingsThe main results suggest that concentration and competition affect financial development. In particular, an increase in bank concentration may inhibit the country's financial development, due to the lack of competition. Our results do not confirm the controversy between concentration and competition, suggesting that concerning financial development, concentration is the reverse of competition.Practical implicationsThe results of this study add a new perspective on banking market power: a financial system concentrated or uncompetitive constrains financial development.Originality/valueThe literature that combines the investigation of the effects of banking market structure (concentration) and banking market conduct (competition) on financial development is scarce. Although a concentrated banking sector can reduce competition through barriers to new entrants (which could expand financial services offer), it is also true that a concentrated banking sector can be competitive. In order to avoid the controversy, our paper chooses to look into a comprehensive approach considering independent measures of bank concentration and bank competition, which together refer to the banking framework.
Globalization and female economic participation in MINT and BRICS countries
Authors: Osinubi Tolulope , Asongu Simplice 
Published: October 2020

PurposeThis study examines the effect of globalization on female economic participation (FEP) in MINT (Mexico, Indonesia, Nigeria and Turkey) and BRICS (Brazil, Russia, India, China and South Africa) countries between 2004 and 2018.Design/methodology/approachFour measures of globalization are employed and sourced from KOF globalization index, 2018, while the female labour force participation rate is a proxy for FEP. The empirical evidence is based on the Pooled Mean Group (PMG) estimator.FindingsThe findings of the PMG estimator from the Panel ARDL method reveal that political and overall globalization in MINT and BRICS countries have a positive impact on FEP, whereas social globalization exerts a negative impact on FEP in the long-run. It is observed that economic globalization has no long-run effect on FEP. Contrarily, all the measures of globalization reflect no short-run effect on FEP. This supports the argument that globalization has no immediate effect on FEP. Thus, it is recommended that both MINT and BRICS countries should find a way of improving the process of globalization generally to empower women to be involved in economic activities.Originality/valueThis study complements the extant literature by focusing on how globalization dynamics influence FEP in the MINT and BRICS countries.
A model for digital development of SMEs: an interaction-based approach
Authors: Depaoli Paolo , Za Stefano , Scornavacca Eusebio 
Published: October 2020

PurposeE-business maturity models have been widely used to guide the process of digital transformation of business. However, these models have been criticized for their predominantly technocentric approach, which is poorly suited for SMEs. The purpose of this paper is to produce a holistic, nonlinear e-business development model for SMEs that takes into account the interactions of the organization in the pursuit of its business objectives.Design/methodology/approachBased on the literature review, this paper proposes a nonlinear digital development model for SMEs that factors in the interactions between digital technologies and organizational processes. The model is applied to three cases using a qualitative research approach.FindingsThe analysis of the cases demonstrates how the model recognizes that SMEs have an agile and flexible operating structure and reduces the role of technology in order to refocus the entrepreneurs on the relationship among business goals, organizational capabilities and communication requirements.Practical implicationsThe proposed model helps the business owners to clarify and better understand what kind of interaction levels exist between the relevant actors in the pursuit of the firm's goals and to identify the best mix of digital and nondigital communication methods to support their work. It also assists policymakers to overcome technological bias when assessing the current state of play of e-business development in the SME universe and formulate actions that support the development of digitally oriented SMEs.Originality/valueThis paper proposes a novel, nonlinear, interaction-based development model for SMEs in order to complement previous technocentric models focusing mainly on a mechanist perspective of e-business maturity models.
Comparative genomics of pyrophilous fungi reveals a link between fire events and developmental genes
Authors: Steindorff Andrei S. , Carver Akiko , Calhoun Sara , Stillman Kyra , Liu Haowen , Lipzen Anna , He Guifen , Yan Mi , Pangilinan Jasmyn , LaButti Kurt , Ng Vivian , Bruns Thomas D. , Grigoriev Igor V. 
Published: October 2020

SummaryForest fires generate a large amount of carbon that remains resident on the site as dead and partially ‘pyrolysed’ (i.e. burnt) material that has long residency times and constitutes a significant pool in fire‐prone ecosystems. In addition, fire‐induced hydrophobic soil layers, caused by condensation of pyrolysed waxes and lipids, increase post‐fire erosion and can lead to long‐term productivity losses. A small set of pyrophilous fungi dominate post‐fire soils and are likely to be involved with the degradation of all these compounds, yet almost nothing is currently known about what these fungi do or the metabolic processes they employ. In this study, we sequenced and analysed genomes from fungi isolated after Rim fire near Yosemite National Park in 2013 and showed the enrichment/expansion of CAZymes and families known to be involved in fruiting body initiation when compared to other basidiomycete fungi. We found gene families potentially involved in the degradation of the hydrophobic layer and pyrolysed organic matter, such as hydrophobic surface binding proteins, laccases (AA1_1), xylanases (GH10, GH11), fatty acid desaturases and tannases. Thus, pyrophilous fungi are important actors to restate the soil's functional capabilities.
‘We Wanted to be Free as a Nation, and We Wanted to be Free as Women’: Decolonisation, Nationalism and Women's Liberation in Zimbabwe, 1979–851
Authors: Law Kate 
Published: October 2020

ABSTRACTThis article examines one of the most intractable problems that a newly independent nation encounters; the dissonance between the rhetoric of a revolutionary movement and its subsequent treatment of women in nationalist and supposedly decolonial projects. In drawing on interviews and archival research carried out in periodicals, newspapers and Hansard, the article examines the optimism, disillusionment and betrayal of Zimbabwe's women in the first decade of independence. Exploring women's variegated roles during the country's war of independence, this article argues that many women believed that their participation in national liberation would be a precursor to a broader programme of cultural and societal emancipation. Yet, as is shown, governmental thinking placed women as consumers and not producers of new nationalist culture. In particular, the grim reality of the situation was unambiguously shown just three years into independence through ‘Operation Clean‐Up’, whereby thousands of women in Zimbabwe's main cities of Harare and Bulawayo were indiscriminately detained with state machinery arguing that the women were prostitutes, vagrants and beggars. A blatant effort to curtail women's autonomy in urban spaces, the machinations of ‘Operation Clean‐up’ demonstrated an uneasy coherence between colonial and post‐colonial thinking regarding the ‘appropriate’ place for women in the new nation.