bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bakterielle Mechanismen der marinen Polysaccharidverwertung
Authors: Schweder Thomas , Bornscheuer Uwe , Hehemann Jan-Hendrik , Amann Rudolf 
Published: November 2020

AbstractThe oceans have been compared to a “global heterotrophic digester”. This is due to the high productivity of microalgae and the rapid turnover of the produced biomass by microbes. A major part of the algal biomass consists of diverse polysaccharides which belong to the most complex polymer structures in nature. These marine sugars are decomposed by specialized bacteria, mainly of the phyla Bacteroidetes and Gammaproteobacteria, which possess dedicated conserved gene clusters encoding a remarkable diversity of carbohydrate-active enzymes.
Profile control performance and field application of preformed particle gel in low-permeability fractured reservoir
Authors: Xu Bin , Wang Yan 
Published: November 2020

AbstractWater well profile control is the main way to control water channeling in low-permeability fractured reservoirs, and preformed particle gels (PPGs) are commonly used. A preformed particle gel was prepared and the synthesis conditions were optimized. The temperature and salt resistant performance, plugging performance were investigated and the field application was tested. The results showed PPGs exhibited good temperature and salt resistant performance. PPGs with larger particle size showed greater plugging strength but shorter valid period. Field tests suggested that multiple rounds of profile control should be employed using PPGs with different particle sizes to achieve deep profile control.
Toward an understanding of the relation between gene regulation and 3D genome organization
Authors: Tian Hao , Yang Ying , Liu Sirui , Quan Hui , Gao Yi Qin 
Published: November 2020

BackgroundHigh‐order chromatin structure has been shown to play a vital role in gene regulation. Previously we identified two types of sequence domains, CGI (CpG island) forest and CGI prairie, which tend to spatially segregate, but to different extent in different tissues. Here we aim to further quantify the association of domain segregation with gene regulation and therefore differentiation.MethodsBy means of the published RNA‐seq and Hi‐C data, we identified tissue‐specific genes and quantitatively investigated how their regulation is relevant to chromatin structure. Besides, two types of gene networks were constructed and the association between gene pair co‐regulation and genome organization is discussed.ResultsWe show that compared to forests, tissue‐specific genes tend to be enriched in prairies. Highly specific genes also tend to cluster according to their functions in a relatively small number of prairies. Furthermore, tissue‐specific forest‐prairie contact formation was associated with the regulation of tissue‐specific genes, in particular those in the prairie domains, pointing to the important role of gene positioning, in the linear DNA sequence as well as in 3D chromatin structure, in gene regulatory network formation.ConclusionWe investigated how gene regulation is related to genome organization from the perspective of forest‐prairie spatial interactions. Since unlike compartments A and B, forest and prairie are identified solely based on sequence properties. Therefore, the simple and uniform framework (forest‐prairie domain segregation) provided here can be utilized to further understand the chromatin structure changes as well as the underlying biological significances in different stages, such as tumorgenesis.
A low-to-high friction transition in gradient nano-grained Cu and Cu-Ag alloys
Authors: Chen Xiang , Han Zhong 
Published: November 2020

AbstractA unique low-to-high friction transition is observed during unlubricated sliding in metals with a gradient nano-grained (GNG) surface layer. After persisting in the low-friction state (0.2–0.4) for tens of thousands of cycles, the coefficients of friction in the GNG copper (Cu) and copper-silver (Cu-5Ag) alloy start to increase, eventually reaching a high level (0.6–0.8). By monitoring the worn surface morphology evolution, wear-induced damage accumulation, and worn subsurface structure evolution during sliding, we found that the low-to-high friction transition is strongly correlated with distinct microstructural instabilities induced by vertical plastic deformation and wear-off of the stable nanograins in the subsurface layer. A very low wear loss of the GNG samples was achieved compared with the coarse-grained sample, especially during the low friction stage. Our results suggest that it is possible to postpone the initiation of low-to-high friction transitions and enhance the wear resistance in GNG metals by increasing the GNG structural stability against grain coarsening under high loading.
Heterogeneity in SIR epidemics modeling: superspreaders and herd immunity
Authors: Szapudi Istvan 
Published: November 2020

AbstractDeterministic epidemic models, such as the Susceptible-Infected-Recovered (SIR) model, are immensely useful even if they lack the nuance and complexity of social contacts at the heart of network science modeling. Here we present a simple modification of the SIR equations to include the heterogeneity of social connection networks. A typical power-law model of social interactions from network science reproduces the observation that individuals with a high number of contacts, “hubs” or “superspreaders”, can become the primary conduits for transmission. Conversely, once the tail of the distribution is saturated, herd immunity sets in at a smaller overall recovered fraction than in the analogous SIR model. The new dynamical equations suggest that cutting off the tail of the social connection distribution, i.e., stopping superspreaders, is an efficient non-pharmaceutical intervention to slow the spread of a pandemic, such as the Coronavirus Disease 2019 (COVID-19).
Numerical investigation on epi-off crosslinking effects on porcine corneas
Authors: Cornaggia Aram , Boschetti Federica , Mazzotta Cosimo , Pandolfi Anna 
Published: November 2020

AbstractExperimental inflation tests, conducted on 90 pig corneas before and after corneal collagen crosslinking (CXL) treatment, are simulated with the finite element method. The experimental sample consists of five groups of corneas treated with different UV-A irradiation times (2.5, 5, 10, 15, and 20 min) at constant irradiance 9 mW/cm2. The linear elastic shell theory is used to estimate the equivalent material stiffness of the corneas, revealing that it increases with the exposure time in CXL corneas. In the view of numerical simulations, a simple mechanical model assuming piecewise constant elastic modulus across the corneal thickness is introduced, to estimate the effective increment of the material stiffness in the anterior stroma and the effective depth of the stiffness increment. The two effective quantities are used in the finite element models to simulate the post-CXL tests. Numerical models are able to describe the mechanical effects of CXL in the cornea. The increment of equivalent material stiffness has to be ascribed to a localized increment of the material stiffness in the anterior layers of the cornea, while the posterior layers preserve the original material stiffness. According to the simplified model, the increment of the material stiffness of the anterior cornea increases with the irradiation dose, while the effective reinforcement depth decreases with the irradiation dose. This trend, predicted by a simple mechanical model by imposing equilibrium and compatibility, has been verified by the numerical calculations that captured the global mechanical response of the corneas in untreated and post-CXL conditions.
3D Bioprinting of Cardiovascular Tissues for In Vivo and In Vitro Applications Using Hybrid Hydrogels Containing Silk Fibroin: State of the Art and Challenges
Authors: Vettori Laura , Sharma Poonam , Rnjak-Kovacina Jelena , Gentile Carmine 
Published: November 2020

Abstract
Purpose of Review
3D bioprinting of cardiovascular tissues for in vitro and in vivo applications is currently investigated as a potential solution to better mimic the microenvironment typical of the human heart. However, optimal cell viability and tissue vascularization remain two of the main challenges in this regard. Silk fibroin (SF) as a natural biomaterial with unique features supports cell survival and tissue vascularization. This review aims to evaluate the potential of hydrogels containing SF in 3D bioprinting of cardiac tissue that better recapitulate the native cardiac microenvironment.

Recent Findings
SF hydrogels spontaneously develop nanocrystals, which limit their use for 3D bioprinting applications. Nevertheless, the printability of SF is improved in hybrid hydrogels by mixing it with other natural polymers (such as alginate and gelatin). This is achieved by adding SF with other polymers or by crosslinking it by peroxidase catalysis (i.e., with alginate). Compared to only SF-based hydrogels, hybrid hydrogels provide a durable bioprinted construct with improved mechanical stability and biological properties. To date, studies using cardiac cells in bioprinted SF constructs are yet to be performed.

Summary
Mixing SF with other polymers in bioprinted hybrid hydrogels improves the printability and durability of 3D bioprinted tissues. Studies using these hydrogels with cardiac cells will be required to evaluate the biocompatibility of SF hybrid hydrogels and to establish their potential use for cardiovascular applications.
Outcomes of Occipital Nerve Stimulation for Craniofacial Pain Syndromes
Authors: Magown Philippe , Becker Werner J , Kiss Zelma HT 
Published: November 2020

ABSTRACT:Objectives:Occipital nerve regional stimulation (ONS) is reported to improve pain in several studies. We examined long-term pain and functional outcomes of ONS in an open-label prospective study.Methods:Patients with medically refractory and disabling craniofacial pain were prospectively selected for ONS. Primary outcome was a change in mean daily pain intensity on the numeric pain rating scale (NPRS) at 6 months. Secondary outcomes included changes in NPRS, Headache Impact Test-6 (HIT-6), Migraine Disability Assessment (MIDAS), Pain Disability Index (PDI), Center for Epidemiologic Studies Depression Scale – Revised (CESD-R), and Short Form-36 version 2 (SF36) at last follow-up.Results:Thirteen patients (mean age 49.7 ± 8.4) diagnosed with occipital neuralgia (6), hemicrania continua (2), persistent idiopathic facial pain (2), post-traumatic facial pain (1), cluster headache (1), and chronic migraine (1) were enrolled. Mean NPRS improved by 2.1 ± 2.1 at 6 months and 2.1 ± 1.9 at last follow-up (23.5 ± 18.1 months). HIT-6 decreased by 8.7 ± 8.8, MIDAS decreased by 61.3 ± 71.6, and PDI decreased by 17.9 ± 18. SF36 physical functioning, bodily pain, and social functioning improved by 16.4 ± 19.6, 18.0 ± 31.6, and 26.1 ± 37.3, respectively. Moderate to severe headache days (defined as ≥50% of baseline mean NPRS) were reduced by 8.9 ± 10.2 days per month with ONS.Conclusion:ONS reduced the long-term NPRS and moderate–severe monthly headache days by 30% and improved functional outcomes and quality of life. A prospective registry for ONS would be helpful in accumulating a larger cohort with longer follow-up in order to improve the use of ONS.
Trajectories of functioning in a population-based sample of veterans: contributions of moral injury, PTSD, and depression
Authors: Maguen Shira , Griffin Brandon J. , Copeland Laurel A. , Perkins Daniel F. , Richardson Cameron B. , Finley Erin P. , Vogt Dawne 
Published: November 2020

AbstractBackgroundAlthough research has shown that exposure to potentially traumatic and morally injurious events is associated with psychological symptoms among veterans, knowledge regarding functioning impacts remains limited.MethodsA population-based sample of post-9/11 veterans completed measures of intimate relationship, health, and work functioning at approximately 9, 15, 21, and 27 months after leaving service. Moral injury, posttraumatic stress, and depression were assessed at ~9 months post-separation. We used Latent Growth Mixture Models to identify discrete classes characterized by unique trajectories of change in functioning over time and to examine predictors of class membership.ResultsVeterans were assigned to one of four functioning trajectories: high and stable, high and decreasing, moderate and increasing, and moderate and stable. Whereas posttraumatic stress, depression, and moral injury associated with perpetration and betrayal predicted worse outcomes at baseline across multiple functioning domains, moral injury associated with perpetration and depression most reliably predicted assignment to trajectories characterized by relatively poor or declining functioning.ConclusionsMoral injury contributes to functional problems beyond what is explained by posttraumatic stress and depression, and moral injury due to perpetration and depression most reliably predicted assignment to trajectories characterized by functional impairment over time.
Behavioral activation therapy for depression is associated with a reduction in the concentration of circulating quinolinic acid
Authors: Savitz Jonathan , Ford Bart N. , Yeh Hung-Wen , Akeman Elisabeth , Cosgrove Kelly , Clausen Ashley N. , Martell Christopher , Kirlic Namik , Santiago Jessica , Teague T. Kent , Irwin Michael R. , Paulus Martin P. , Aupperle Robin L. 
Published: November 2020

AbstractBackgroundAn inflammation-induced imbalance in the kynurenine pathway (KP) has been reported in major depressive disorder but the utility of these metabolites as predictive or therapeutic biomarkers of behavioral activation (BA) therapy is unknown.MethodsSerum samples were provided by 56 depressed individuals before BA therapy and 29 of these individuals also provided samples after 10 weeks of therapy to measure cytokines and KP metabolites. The PROMIS Depression Scale (PROMIS-D) and the Sheehan Disability Scale were administered weekly and the Beck depression inventory was administered pre- and post-therapy. Data were analyzed with linear mixed-effect, general linear, and logistic regression models. The primary outcome for the biomarker analyses was the ratio of kynurenic acid to quinolinic acid (KynA/QA).ResultsBA decreased depression and disability scores (p's < 0.001, Cohen's d's > 0.5). KynA/QA significantly increased at post-therapy relative to baseline (p < 0.001, d = 2.2), an effect driven by a decrease in QA post-therapy (p < 0.001, uncorrected, d = 3.39). A trend towards a decrease in the ratio of kynurenine to tryptophan (KYN/TRP) was also observed (p = 0.054, uncorrected, d = 0.78). Neither the change in KynA/QA, nor baseline KynA/QA were associated with response to BA therapy.ConclusionThe current findings together with previous research show that electronconvulsive therapy, escitalopram, and ketamine decrease concentrations of the neurotoxin, QA, raise the possibility that a common therapeutic mechanism underlies diverse forms of anti-depressant treatment but future controlled studies are needed to test this hypothesis.
Supplementation of c-type natriuretic peptide during in vitro growth period benefits the development of murine preantral follicles
Authors: Ang Li , Haixia Cao , Hongxia Li , Ruijiao Li , Xingping Guo , Huaixiu Wang 
Published: November 2020

SummaryThe present study investigated the effects of c-type natriuretic peptide (CNP) on the development of murine preantral follicles during in vitro growth (IVG). Preantral follicles isolated from ovaries of Kunming mice were cultured in vitro. In the culture system, CNP was supplemented in the experimental groups and omitted in the control groups. In Experiment 1, CNP was only supplemented at the early stage and follicle development was evaluated. In Experiments 2 and 3, CNP was supplemented during the whole period of in vitro culture. In Experiment 2, follicle development and oocyte maturity were evaluated. In Experiment 3, follicle development and embryo cleavage after in vitro fertilization (IVF) were assessed. The results showed that in the control groups in all three experiments, granulosa cells migrated from within the follicle and the follicles could not reach the antral stage. In the experimental groups in all three experiments, no migration of granulosa cells was observed and follicle development was assessed as attaining the antral stage, which was significantly superior to that of the control group (P < 0.0001). Oocyte meiotic arrest was effectively maintained, hence giving good developmental competence. In conclusion, CNP supplementation in the culture system during IVG benefited the development of murine preantral follicles.
Extent and nutritional quality of foods and beverages to which children are exposed in Colombian TV food advertising
Authors: Velasquez Alcides , Mora-Plazas Mercedes , Gómez Luis Fernando , Taillie Lindsey S , Dillman Carpentier Francesca R 
Published: November 2020

AbstractObjective:To examine children’s exposure to food and beverage advertising across a year of Colombian television based on whether products exceed Pan-American Health Organization (PAHO)-defined nutrient thresholds.Design:Nutritional information was obtained for all foods and beverages advertised and used to categorise each product according to the product category (e.g. beverage, snack food) and nutritional quality based on the PAHO model for identifying products in excess of free sugars, Na or saturated fat or containing non-caloric sweeteners or trans-fat. Television audience ratings data were used to derive the average child audience (unique child viewers) per ad and the number of times ads were seen by children in a single week (weekly impressions) based on product category and nutritional quality.Setting:All food and beverage ads on cable and over-the-air TV in Colombia in 2017.Participants:N/A.Results:Of all instances of TV ads, 89·3 % were of unhealthy products. A larger proportion of male and female children, as well as children from low (88·01 %), mid (89·10 %) and high (89·10 %) socio-economic status, are exposed to advertising of unhealthy products, but no significant difference was found between these proportions.Conclusion:The majority of foods and beverages advertised to Colombian children are unhealthy. These findings highlight a need to implement statutory measures to reduce children’s exposure to unhealthy food advertising in Colombia, as obesity and overweight have been increasing among school-age children in Colombia, and exposure to television advertising of unhealthy foods is a known contributor to children’s food intake and obesity.
Fires Represent an Important Source of Carbon Emissions in Mexico
Authors: Corona‐Núñez Rogelio O. , Li Fangjun , Campo Julio E. 
Published: November 2020

AbstractBiomass burning, including fires, has been identified as the largest source of primary fine carbonaceous particles in the troposphere and one of the major drivers of global carbon (C) cycle, cloud properties, and climate. Most of the global C emissions happen in the Pantropic region. Modeling estimates suggest an increase in Mexican fire frequencies, intensity, and C emission to the atmosphere. However, no study has combined field and satellite data to estimate C emissions by fires for any tropical country. Here, we present an approach to estimate the spatiotemporal variability of fires and its total C emissions in Mexico with the associated climate that combines national fire inventories with remote sensing. We provide evidence that tropical ecosystems, particularly tropical forests at Yucatan Peninsula, Pacific Coast, and Gulf of Mexico, provide the largest C emissions with high fire densities. We found evidence to contrast the relationships of the interannual and intra‐annual variability of C emissions with immediate and lagged climate effects such as El Niño and La Niña events. Data also indicate that C emissions from fires are up to 11 times higher than those from deforestation and account 19% of the total National CO2 emissions and 5% of the total Pantropical C emissions. Because fires are related to climate change, we encourage future studies to focus on climate fire feedbacks for implementing adequate climate mitigation strategies.
FGF401 and vinorelbine synergistically mediate antitumor activity and vascular normalization in FGF19-dependent hepatocellular carcinoma
Authors: Huynh Hung , Prawira Aldo , Le Thi Bich Uyen , Vu Thanh Chung , Hao Huai-Xiang , Huang Alan , Wang Youzhen , Porta Diana Graus 
Published: November 2020

AbstractHepatocellular carcinoma (HCC) is a lethal cancer with limited therapeutic options, and standard therapy with sorafenib provides only modest survival benefits. Fibroblast growth factor 19 (FGF19) has been proposed as a driver oncogene, and targeting its receptor, FGFR-4, may provide a better alternative to standard therapy for patients with FGF19-driven tumors. Sixty-three HCC patient-derived xenograft (PDX) models were screened for FGF19 expression. Mice bearing high and low FGF19-expressing tumors were treated with FGF401 and/or vinorelbine, and the antitumor activity of both agents was assessed individually and in combination. Tumor vasculature and intratumoral hypoxia were also examined. High FGF19 expression was detected in 14.3% (9 of 63) of the HCC models tested and may represent a good target for HCC treatment. FGF401 potently inhibited the growth of high FGF19-expressing HCC models regardless ofFGF19gene amplification. Furthermore, FGF401 inhibited the FGF19/FGFR-4 signaling pathway, cell proliferation, and hypoxia, induced apoptosis and blood vessel normalization and prolonged the overall survival (OS) of mice bearing high FGF19 tumors. FGF401 synergistically acted with the microtubule-depolymerizing drug vinorelbine to further suppress tumor growth, promote apoptosis, and prolong the OS of mice bearing high FGF19 tumors, with no evidence of increased toxicity. Our study suggests that a subset of patients with high FGF19-expressing HCC tumors could benefit from FGF401 or FGF401/vinorelbine treatment. A high level of FGF19 in a tumor may serve as a potential biomarker for patient selection.
CircCNTNAP3-TP53-positive feedback loop suppresses malignant progression of esophageal squamous cell carcinoma
Authors: Wang Hui , Song Xuming , Wang Yajing , Yin Xuewen , Liang Yingkuan , Zhang Te , Xu Lin , Jiang Feng , Dong Gaochao 
Published: November 2020

AbstractMutation or downregulation of p53 (encoded by TP53) accelerates tumorigenesis and malignant progression in esophageal squamous cell carcinoma (ESCC). However, it is still unknown whether circular RNAs (circRNAs), a novel class of endogenous noncoding RNAs, participate in the regulation of this progress. In this study, we explored the expression profiles of circRNAs in three paired samples of ESCC and identified cCNTNAP3, which is a circRNA that originates from the CNTNAP3 gene transcript and is highly expressed in normal human esophageal tissue. However, we found that the cCNTNAP3 expression level was significantly downregulated in ESCC tissues. In vitro and in vivo studies revealed that cCNTNAP3 inhibited proliferation and increased apoptosis in p53 wild-type ESCC cells, but not in mutant cells. Mechanistically, we found that cCNTNAP3 promotes the expression of p53 by sponging miR-513a-5p. Rescue assay confirmed that the suppressive function of cCNTNAP3 was dependent on miR-513a-5p. We also observed that p53/RBM25 participated in the formation of cCNTNAP3, which implied the existence of a positive feedback loop between cCNTNAP3 and p53. Furthermore, the downregulation of cCNTNAP3 was significantly correlated with later T stage and thus can serve as an independent risk factor for the overall survival of patients with p53 wild-type ESCC. In conclusion, the cCNTNAP3-TP53 positive feedback loop may provide a potential target for the management of ESCC, which also reveals the important role of circRNAs in the regulation of p53.