bluuebunny/crossref_metadata_2025_split huggingface dataset:
Warming the phycosphere: Differential effect of temperature on the use of diatom‐derived carbon by two copiotrophic bacterial taxa
Authors: Arandia‐Gorostidi Nestor , Alonso‐Sáez Laura , Stryhanyuk Hryhoriy , Richnow Hans H. , Morán Xosé Anxelu G. , Musat Niculina 
Published: February 2020

SummaryHeterotrophic bacteria associated with microphytoplankton, particularly those colonizing the phycosphere, are major players in the remineralization of algal‐derived carbon. Ocean warming might impact dissolved organic carbon (DOC) uptake by microphytoplankton‐associated bacteria with unknown biogeochemical implications. Here, by incubating natural seawater samples at three different temperatures, we analysed the effect of experimental warming on the abundance and C and N uptake activity of Rhodobacteraceae and Flavobacteria, two bacterial groups typically associated with microphytoplankton. Using a nano‐scale secondary ion mass spectrometry (nanoSIMS) single‐cell analysis, we quantified the temperature sensitivity of these two taxonomic groups to the uptake of algal‐derived DOC in the microphytoplankton associated fraction with 13C‐bicarbonate and 15N‐leucine as tracers. We found that cell‐specific 13C uptake was similar for both groups (~0.42 fg C h−1 μm−3), but Rhodobacteraceae were more active in 15N‐leucine uptake. Due to the higher abundance of Flavobacteria associated with microphytoplankton, this group incorporated fourfold more carbon than Rhodobacteraceae. Cell‐specific 13C uptake was influenced by temperature, but no significant differences were found for 15N‐leucine uptake. Our results show that the contribution of Flavobacteria and Rhodobacteraceae to C assimilation increased up to sixfold and twofold, respectively, with an increase of 3°C above ambient temperature, suggesting that warming may differently affect the contribution of distinct copiotrophic bacterial taxa to carbon cycling.
Protein expression in the obligate hydrocarbon‐degrading psychrophile Oleispira antarctica RB‐8 during alkane degradation and cold tolerance
Authors: Gregson Benjamin H. , Metodieva Gergana , Metodiev Metodi V. , Golyshin Peter N. , McKew Boyd A. 
Published: February 2020

SummaryIn cold marine environments, the obligate hydrocarbon‐degrading psychrophile Oleispira antarctica RB‐8, which utilizes aliphatic alkanes almost exclusively as substrates, dominates microbial communities following oil spills. In this study, LC–MS/MS shotgun proteomics was used to identify changes in the proteome induced during growth on n‐alkanes and in cold temperatures. Specifically, proteins with significantly higher relative abundance during growth on tetradecane (n‐C14) at 16°C and 4°C have been quantified. During growth on n‐C14, O. antarctica expressed a complete pathway for the terminal oxidation of n‐alkanes including two alkane monooxygenases, two alcohol dehydrogenases, two aldehyde dehydrogenases, a fatty‐acid‐CoA ligase, a fatty acid desaturase and associated oxidoreductases. Increased biosynthesis of these proteins ranged from 3‐ to 21‐fold compared with growth on a non‐hydrocarbon control. This study also highlights mechanisms O. antarctica may utilize to provide it with ecological competitiveness at low temperatures. This was evidenced by an increase in spectral counts for proteins involved in flagella structure/output to overcome higher viscosity, flagella rotation to accumulate cells and proline metabolism to counteract oxidative stress, during growth at 4°C compared with 16°C. Such species‐specific understanding of the physiology during hydrocarbon degradation can be important for parameterizing models that predict the fate of marine oil spills.
The Accuracy and Repeatability of Reconstructing Single Bullet Impacts Using the 2D Ellipse Method
Authors: Walters Miguel , Liscio Eugene 
Published: February 2020

AbstractWhen a bullet punctures a surface, it leaves behind a bullet impact, which can be analyzed in order to determine the origin and trajectory path from which a bullet was discharged using many different scientific methods to reconstruct a shooting scene. The purpose of the research was to test the accuracy and repeatability of reconstructing the impact angle of single bullet impacts using the ellipse method. The research was conducted by firing various calibers of ammunition into drywall panels positioned at varying angles of incidence from 88° until ricochet occurred. This was done in order to determine which caliber type and angle of incidence are most accurate and precise for reconstructing a shooting scene. The study examined four caliber types fired into drywall panels, with 5 repeated shots for each of the 11 angles of incidence (n = 220). Furthermore, 31 participants partook in the study to estimate a bullet impact angle of incidence utilizing the ellipse method. The results show that the best performance (accuracy and repeatability) is seen with the measurements of the 0.45 caliber ammunition. When angle of incidences is low (<64°), the performance (accuracy and repeatability) was seen to be better in all caliber ammunitions. Overall, the data provided for single bullet impacts deposited in drywall show that the ellipse method is useful in providing measurements for most crime scene reconstruction purposes and has also demonstrated that results vary depending on the type of ammunition, firearm, and angle of incidence being examined.
Diversity of bacteria in different life stages and their impact on the development and reproduction of Zeugodacus tau (Diptera: Tephritidae)
Authors: Noman Md. Shibly , Shi Ge , Liu Li‐Jun , Li Zhi‐Hong 
Published: February 2020

AbstractFruit flies usually harbor diverse communities of bacteria in their digestive systems, which are known to play a significant role in their fitness. However, little information is available on Zeugodacus tau, a polyphagous pest worldwide. This study reports the first extensive analysis of bacterial communities in different life stages and their effect on the development and reproduction of laboratory‐reared Z. tau. Cultured bacteria were identified using the conventional method, and all bacteria were identified by high‐throughput technologies (16S ribosomal RNA gene sequencing of V3‒V4 region). A total of six bacterial phyla were identified in larvae, pupae, and male and female adult flies, which were distributed into 14 classes, 32 orders, 58 families and 96 genera. Proteobacteria was the most represented phylum in all the stages except larvae. Enterobacter, Klebsiella, Providencia, and Pseudomonas were identified by conventional and next‐generation sequencing analysis in both male and female adult flies, and Enterobacter was found to be the main genus. After being fed with antibiotics from the first instar larvae, bacterial diversity changed markedly in the adult stage. Untreated flies laid eggs and needed 20 days before oviposition while the treated flies showed ovary development inhibited and were not able to lay eggs, probably due to the alteration of the microbiota. These findings provide the cornerstone for unexplored research on bacterial function in Z. tau, which will help to develop an environmentally friendly management technique for this kind of harmful insect.
Impact of defelonizing drug possession on recidivism
Authors: Bird Mia , Nguyen Viet , Grattet Ryken 
Published: February 2020

Research SummaryCalifornia's Proposition 47 (Prop 47), passed in November 2014, sought to scale back punishment for selected drug and property offenses, making them misdemeanors rather than felonies. Although others have examined the impacts of Proposition 47 on crime rates, here we examine the impacts on a range of recidivism outcomes specifically for individuals convicted for drug possession offenses. We focus on the defelonization of drug possession because nationally evidence suggests that public and policy maker sympathy for reducing incarceration use is greatest for nonviolent drug offenders. Thus, the greatest relevance of Proposition 47 to the nation lies in its strategy of defelonizing drug possession. We draw on an ongoing data collection effort on criminal justice populations in 12 California counties and link these data to rearrest and reconviction data from county and state‐level sources. We find people who received drug possession convictions after Prop 47 had lower overall rearrest and reconviction rates than people with comparable convictions and criminal histories released prior to the proposition. Overall reductions in recidivism rates for Prop 47 drug offenders were driven by reductions in rearrest and reconviction for drug possession offenses. These findings are robust to a variety of specifications and sensitivity analyses. When we complicate the narrative of declines in nonviolent recidivism, however, we find evidence of a 2.8‐percentage‐point increase in rearrest for crimes against persons, mainly assaults and domestic violence. Reconviction rates for crimes against persons for drug possession offenders also increased by 1.1 percentage points.Policy ImplicationsThese findings add to the growing body of evidence that a targeted reform like Prop 47 does not result in increases in most measures of reoffending for people convicted of drug possession. Although we cannot rule out that the declines we observe in rearrest and reconviction are the result of behavioral change among offenders, it seems likely that those declines are partly, perhaps mostly, the result of changing decision‐making among law enforcement and prosecutors. Converting drug possession to be chargeable only as a misdemeanor has significantly reduced the likelihood individuals convicted of drug possession will return to jail or prison once they have been released and therefore stemmed some of the flow of people with such offenses through the system. Evidence from California, however, shows that there can be small increases in crimes against persons, which provides a cautionary note and points to the need to consider interventions that do not involve criminal justice responses in response to the negative aspects of drug use. Our conclusion discusses considerations in applying our findings to other states that have defelonized drug possession.
Examining the state repression‐terrorism nexus: Dynamic relationships among repressive counterterrorism actions, terrorist targets, and deadly terrorist violence in Israel
Authors: Hsu Henda Y. , McDowall David 
Published: February 2020

Research SummaryThis study assesses the impact of state repressive counterterrorism actions on terrorists’ targeting and lethality of the terrorism landscape in Israel. Using systematic data on government responses to terrorism and an empirical model that addresses reciprocal relationships, we analyze dynamical interactions between types of government repression with attacks against civilian or government targets, and deadly terrorist violence. Contrary to public policy pronouncements of forcefully fighting terrorism to eradicate terrorist threats, our results suggest that repressive counterterrorism actions by the state increase terror and deadly violence. Importantly, the results indicate that these unintended escalatory or backlash effects are dependent on the scope of government repression, the type of terrorist targets, and the lethality of terrorism.Policy ImplicationsOur findings provide insight into particular behaviors and exchanges within the state repression–terrorism nexus that evoke and prolong terrorist violence. Centered on key aspects of terrorist activities involving target choice and deadly violence, our ecological study contributes to the development of a systematic understanding of specific impacts of government policies on terrorism. In all, constructing an empirically rigorous evidence base for terrorism prevention policies and practices is essential for ensuring the protection of human lives and public resources from terrorism.
Widespread microbial mercury methylation genes in the global ocean
Authors: Villar Emilie , Cabrol Léa , Heimbürger‐Boavida Lars‐Eric 
Published: February 2020

SummaryMethylmercury is a neurotoxin that bioaccumulates from seawater to high concentrations in marine fish, putting human and ecosystem health at risk. High methylmercury levels have been found in the oxic subsurface waters of all oceans, but only anaerobic microorganisms have been shown to efficiently produce methylmercury in anoxic environments. The microaerophilic nitrite‐oxidizing bacteria Nitrospina have previously been suggested as possible mercury methylating bacteria in Antarctic sea ice. However, the microorganisms responsible for processing inorganic mercury into methylmercury in oxic seawater remain unknown. Here, we show metagenomic and metatranscriptomic evidence that the genetic potential for microbial methylmercury production is widespread in oxic seawater. We find high abundance and expression of the key mercury methylating genes hgcAB across all ocean basins, corresponding to the taxonomic relatives of known mercury methylating bacteria from Deltaproteobacteria, Firmicutes and Chloroflexi. Our results identify Nitrospina as the predominant and widespread microorganism carrying and actively expressing hgcAB. The highest hgcAB abundance and expression occurs in the oxic subsurface waters of the global ocean where the highest MeHg concentrations are typically observed.
IL‐1β prevents ILC2 expansion, type 2 cytokine secretion, and mucus metaplasia in response to early‐life rhinovirus infection in mice
Authors: Han Mingyuan , Ishikawa Tomoko , Bermick Jennifer R. , Rajput Charu , Lei Jing , Goldsmith Adam M. , Jarman Caitlin R. , Lee Julie , Bentley J. Kelley , Hershenson Marc B. 
Published: February 2020

AbstractBackgroundEarly‐life wheezing‐associated respiratory infection with human rhinovirus (RV) is associated with asthma development. RV infection of 6‐day‐old immature mice causes mucous metaplasia and airway hyperresponsiveness which is associated with the expansion of IL‐13‐producing type 2 innate lymphoid cells (ILC2s) and dependent on IL‐25 and IL‐33. We examined regulation of this asthma‐like phenotype by IL‐1β.MethodsSix‐day‐old wild‐type or NRLP3−/− mice were inoculated with sham or RV‐A1B. Selected mice were treated with IL‐1 receptor antagonist (IL‐1RA), anti‐IL‐1β, or recombinant IL‐1β.ResultsRhinovirus infection induced Il25, Il33, Il4, Il5, Il13, muc5ac, and gob5 mRNA expression, ILC2 expansion, mucus metaplasia, and airway hyperresponsiveness. RV also induced lung mRNA and protein expression of pro‐IL‐1β and NLRP3 as well as cleavage of caspase‐1 and pro‐IL‐1β, indicating inflammasome priming and activation. Lung macrophages were a major source of IL‐1β. Inhibition of IL‐1β signaling with IL‐1RA, anti‐IL‐1β, or NLRP3 KO increased RV‐induced type 2 cytokine immune responses, ILC2 number, and mucus metaplasia, while decreasing IL‐17 mRNA expression. Treatment with IL‐1β had the opposite effect, decreasing IL‐25, IL‐33, and mucous metaplasia while increasing IL‐17 expression. IL‐1β and IL‐17 each suppressed Il25, Il33, and muc5ac mRNA expression in cultured airway epithelial cells. Finally, RV‐infected 6‐day‐old mice showed reduced IL‐1β mRNA and protein expression compared to mature mice.ConclusionMacrophage IL‐1β limits type 2 inflammation and mucous metaplasia following RV infection by suppressing epithelial cell innate cytokine expression. Reduced IL‐1β production in immature animals provides a mechanism permitting asthma development after early‐life viral infection.
Therapeutic use of pulsed electromagnetic field therapy reduces prostate volume and lower urinary tract symptoms in benign prostatic hyperplasia
Authors: Tenuta Marta , Tarsitano Maria G. , Mazzotta Paola , Lucchini Livia , Sesti Franz , Fattorini Giorgio , Pozza Carlotta , Olivieri Valerio , Naro Fabio , Gianfrilli Daniele , Lenzi Andrea , Isidori Andrea M. , Pofi Riccardo 
Published: February 2020

AbstractBackgroundBenign prostatic hyperplasia (BPH) etiology remains poorly understood, but chronic low‐grade inflammation plays a role. Pulsed electromagnetic field therapy (PEMF) (1‐50 Hz) is effective in reducing tissue inflammation.ObjectivesWe designed a pilot study to evaluate the effects of PEMF on prostate volume (PV) in BPH.Materials and MethodsThis is a prospective interventional trial on 27 naive patients with BPH and lower urinary tract symptoms (LUTS). At baseline (V0), all patients had blood tests, transrectal ultrasound, and questionnaires (IPSS, IIEF‐15) and received a perineal PEMF device (Magcell®Microcirc, Physiomed Elektromedizin). PEMF was delivered on perineal area 5 minutes twice daily for 28 days, then (V1) all baseline evaluations were repeated. Afterward, nine patients continued therapy for 3 more months (PT group) and 15 discontinued (FU group). A 4‐month evaluation (V2) was performed in both groups.ResultsA reduction was observed both at V1 and at V2 in PV: PVV0 44.5 mL (38.0;61.6) vs PVV1 42.1 mL (33.7;61.5, P = .039) vs PVV2 41.7mL (32.7;62.8, P = .045). IPSS was reduced both at V1 and at V2: IPSSV0 11 (5.7;23.2) vs IPSSV1 10 (6;16, P = .045) vs IPSSV2 9 (6;14, P = .015). Baseline IPSS was related to IPSS reduction both at V1 (rs = 0.313;P = .003) and at V2 (rs = 0.664;P < .001). PV reduction in patients without metabolic syndrome (ΔPVV1nMetS −4.7 mL, 95%CI −7.3;‐2.0) was greater than in affected patients (ΔPVV1MetS 1.7 mL, 95%CI −2.69;6.1)(P = .017, Relative RiskMetS = 6). No changes were found in gonadal hormones or sexual function.DiscussionPEMF was able to reduce PV after 28 days of therapy. Symptoms improved in a short time, with high compliance and no effects on hormonal and sexual function or any side effects. Patients with moderate‐severe LUTS and without MetS seem to benefit more from this treatment.ConclusionPEMF reduces PV and improves LUTS in a relative short time, in BPH patients. These benefits seem greater in those patients with moderate‐severe LUTS but without MetS.
The Social Life of Robots: The Politics of Algorithms, Governance, and Sovereignty
Authors: Del Casino Vincent J. , House‐Peters Lily , Crampton Jeremy W. , Gerhardt Hannes 
Published: February 2020

AbstractThis symposium aims to broaden the way scholars theorise and empirically treat the increasingly complex relationships between robots and social life, especially in the context of our historically anthropocentric human geographies. The authors of this symposium engage a range of diverse epistemological, ontological, and methodological commitments, but all in some way address the power dynamics and shifting political economies involved in human–robotic interactions as well as possibilities for resisting and overcoming particular forms of domination and oppression. At the same time, the papers present new avenues for conceptualising the rise of robots and robotics and the everyday socio‐spatial relations of contemporary algorithmic life. In a rapidly evolving present and future, where life is increasingly managed in relation to algorithmic imaginaries and automated fantasies, these papers demonstrate the potential for geographers to make significant interventions and contributions to investigate the limits, contradictions, and messy contingencies of socio‐technical assemblages, to trace the shifting spatialities and temporalities of the geographies of algorithmic governance, and to envision radical democratic, post‐capitalist, emancipatory alternatives. These futures are unlikely to be “robot free”, so the question remains how will we build a future set of geographies that acknowledges this reality while also claiming space for the diverse and rich expansion of all forms of life, both human and non‐human?
Type‐I interferon regulates matrix metalloproteinases clearance of the bovine endometrial spheroid
Authors: Rahman Al‐Nur Md. Iftekhar , Yamashita Seiya , Islam Md. Rashedul , Fujihara Taisuke , Yamaguchi Hayato , Kawahara Manabu , Takahashi Masashi , Takahashi Hideyuki , Gotoh Takafumi , Yamauchi Nobuhiko 
Published: February 2020

AbstractThis study investigated the effect of type‐I interferon (IFN) on the expression of matrix metalloproteinases (MMPs) of the bovine endometrial stromal cells (BES) and epithelial cells (BEE). The cells were separated and purified from the caruncles and cultured in DMEM/F‐12 containing 10% fetal bovine serum. Spheroids were generated by using ascorbate. Zymograms of the supernatant showed that BEE predominantly expressed MMP‐9, whereas MMP‐2 was expressed in BES and homo‐spheroids. While MMPs expression was not detected in hetero‐spheroids. Real‐time quantitative PCR revealed that type‐I IFN and P4 suppressed the gene expression of MMP‐2 and MMP‐9 in hetero‐spheroids, respectively. On the other hand, gelatin zymography analysis of the supernatant showed that type‐I IFN strongly promote the clearance of MMPs. While zymograms of the MMPs stocked in the hetero‐spheroids were significantly reduced by type‐I IFN. Phenylmethanesulfonyl fluoride and leupeptin (both are serine proteinase inhibitors) significantly repressed the clearance of MMP‐2 and MMP‐9 induced by type‐I IFN. Moreover, collagen fibers in hetero‐spheroids significantly decreased after the treatment with type‐I IFN. In conclusion, it was suggested that type‐I IFN participate in the tissue remodeling by regulation the clearance of MMPs.
CYP2D6 polymorphism and its impact on the clinical response to metoprolol: A systematic review and meta‐analysis
Authors: Meloche Maxime , Khazaka Michael , Kassem Imad , Barhdadi Amina , Dubé Marie‐Pierre , de Denus Simon 
Published: February 2020

AimsCYP2D6 genetic polymorphisms are associated with metoprolol pharmacokinetics. Whether the clinical response to metoprolol is also affected remains uncertain.MethodsWe conducted a systematic review on the effects of CYP2D6 polymorphism on the clinical response to metoprolol. Searches were conducted using MEDLINE. Meta‐analyses were performed on the impact of CYP2D6‐inferred phenotypes on heart rate (HR) reduction, diastolic (DBP) and systolic (SBP) blood pressure reduction, average daily doses, all‐type adverse events and bradycardia.ResultsOur qualitative assessment indicated inconsistent results in individual studies and endpoints, but CYP2D6 poor metabolizers (PM) generally presented a greater reduction in HR. The meta‐analysis of 15 studies, including a total of 1146 individuals, found a reduction in HR of 3 beats/min (P = .017), and of SBP and DBP by 3 mmHg (P = .0048) for PM compared to non‐PM individuals using similar metoprolol doses. Bradycardia appeared more frequent by 4‐fold for PM, although significant heterogeneity was observed regarding bradycardia, which limits the scope of this finding.ConclusionPatients without any CYP2D6 metabolic capacities appear to have increased reduction in DBP, HR and SBP during metoprolol treatment and may be at a higher risk of bradycardia compared to patients with active CYP2D6 phenotypes. Further prospective data are required to determine whether CYP2D6 is associated with clinical events in patients treated with metoprolol, as well as to demonstrate the clinical utility of an individualized approach of prescribing metoprolol using CYP2D6‐inferred phenotypes.
The ethics of risk displacement in research and public policy
Authors: Vong Gerard , Levinson Meira 
Published: February 2020

AbstractWe identify three distinct ethical problems that can arise with risk displacement. Risk displacement is the shifting of extant risk from one or more individuals to other individual(s) such that the reduction of risk to the first group is causally implicated in increasing risk to the second group. These problems are: concentration of risk in inequitable ways; transfer of risk to already vulnerable or disadvantaged populations; and exercise of undue influence over potential research participants. The first two arise in both public policy and research initiatives, whereas the third is a special concern that only applies to research initiatives. We argue that when one or more of these is of high magnitude, then the study or policy intervention may be ethically wrong. Finally, we conclude that although some risk displacement is ethically permissible, researchers and policymakers still have ethical reasons to reduce the magnitude of these problems.