bluuebunny/crossref_metadata_2025_split huggingface dataset:
Breastfeeding experiences and perspectives on support among Chinese mothers separated from their hospitalized preterm infants: a qualitative study
Authors: Yang Yuanyuan , Brandon Debra , Lu Hong , Cong Xiaomei 
Published: November 2019

Abstract

Background
Chinese mothers of preterm infants often face obstacles to breastfeeding and commonly experience prolonged maternal-infant separation when their high-risk infants are hospitalized in a Neonatal Intensive Care Unit (NICU). This separation hinders mother-infant attachment and the establishment of breastfeeding. Currently, little is known about Chinese mothers’ experiences breastfeeding their preterm infants, or their support needs. The aim of this study was to develop an understanding of mothers’ experiences breastfeeding a hospitalized preterm infant and the support needed to establish a milk supply during the period separation from their infants.

Methods
A qualitative descriptive study was conducted in Beijing in 2017. A total of 11 Chinese mothers were individually interviewed while separated from their infants. The interviews were audio-recorded and transcribed verbatim. A thematic analysis involving a seven-step protocol identified key themes.

Results
Mothers of preterm infants reported physically and mentally challenging breastfeeding experiences during the period they were separated from their babies. They viewed expressing breast milk as integral to their maternal role, even though some found expressing breastmilk exhausting. With little professional support available, the mothers depended upon nonprofessionals to establish breastfeeding.

Conclusions
The study identified the difficulties mothers experienced establishing a milk supply while separated from their preterm infants, and the importance of access to health professional support.
Image analysis-based recognition and quantification of grain number per panicle in rice
Authors: Wu Wei , Liu Tao , Zhou Ping , Yang Tianle , Li Chunyan , Zhong Xiaochun , Sun Chengming , Liu Shengping , Guo Wenshan 
Published: October 2019

Abstract

Background
The number grain per panicle of rice is an important phenotypic trait and a significant index for variety screening and cultivation management. The methods that are currently used to count the number of grains per panicle are manually conducted, making them labor intensive and time consuming. Existing image-based grain counting methods had difficulty in separating overlapped grains.

Results
In this study, we aimed to develop an image analysis-based method to quickly quantify the number of rice grains per panicle. We compared the counting accuracy of several methods among different image acquisition devices and multiple panicle shapes on both Indica and Japonica subspecies of rice. The linear regression model developed in this study had a grain counting accuracy greater than 96% and 97% for Japonica and Indica rice, respectively. Moreover, while the deep learning model that we used was more time consuming than the linear regression model, the average counting accuracy was greater than 99%.

Conclusions
We developed a rice grain counting method that accurately counts the number of grains on a detached panicle, and believe this method can be a huge asset for guiding the development of high throughput methods for counting the grain number per panicle in other crops.
Saccharomyces cerevisiae exhibiting a modified route for uptake and catabolism of glycerol forms significant amounts of ethanol from this carbon source considered as ‘non-fermentable’
Authors: Aßkamp Maximilian R. , Klein Mathias , Nevoigt Elke 
Published: October 2019

Abstract

Background
Due to its inevitable formation during biodiesel production and its relatively high degree of reduction, glycerol is an attractive carbon source for microbial fermentation processes. However, glycerol is catabolized in a fully respiratory manner by the eukaryotic platform organism Saccharomyces cerevisiae. We previously engineered S. cerevisiae strains to favor fermentative metabolism of glycerol by replacing the native FAD-dependent glycerol catabolic pathway with the NAD-dependent ‘DHA pathway’. In addition, a heterologous aquaglyceroporin (Fps1 homolog) was expressed to facilitate glycerol uptake. The current study was launched to scrutinize the formation of S. cerevisiae’s natural fermentation product ethanol from glycerol caused by the conducted genetic modifications. This understanding is supposed to facilitate future engineering of this yeast for fermenting glycerol into valuable products more reduced than ethanol.

Results
A strain solely exhibiting the glycerol catabolic pathway replacement produced ethanol at concentrations close to the detection limit. The expression of the heterologous aquaglyceroporin caused significant ethanol production (8.5 g L−1 from 51.5 g L−1 glycerol consumed) in a strain catabolizing glycerol via the DHA pathway but not in the wild-type background. A reduction of oxygen availability in the shake flask cultures further increased the ethanol titer up to 15.7 g L−1 (from 45 g L−1 glycerol consumed).

Conclusion
The increased yield of cytosolic NADH caused by the glycerol catabolic pathway replacement seems to be a minimal requirement for the occurrence of alcoholic fermentation in S. cerevisiae growing in synthetic glycerol medium. The remarkable metabolic switch to ethanol formation in the DHA pathway strain with the heterologous aquaglyceroporin supports the assumption of a much stronger influx of glycerol accompanied by an increased rate of cytosolic NADH production via the DHA pathway. The fact that a reduction of oxygen supply increases ethanol production in DHA pathway strains is in line with the hypothesis that a major part of glycerol in normal shake flask cultures still enters the catabolism in a respiratory manner.
LIM and cysteine-rich domains 1 (LMCD1) regulates skeletal muscle hypertrophy, calcium handling, and force
Authors: Ferreira Duarte M. S. , Cheng Arthur J. , Agudelo Leandro Z. , Cervenka Igor , Chaillou Thomas , Correia Jorge C. , Porsmyr-Palmertz Margareta , Izadi Manizheh , Hansson Alicia , Martínez-Redondo Vicente , Valente-Silva Paula , Pettersson-Klein Amanda T. , Estall Jennifer L. , Robinson Matthew M. , Nair K. Sreekumaran , Lanner Johanna T. , Ruas Jorge L. 
Published: October 2019

Abstract

Background
Skeletal muscle mass and strength are crucial determinants of health. Muscle mass loss is associated with weakness, fatigue, and insulin resistance. In fact, it is predicted that controlling muscle atrophy can reduce morbidity and mortality associated with diseases such as cancer cachexia and sarcopenia.

Methods
We analyzed gene expression data from muscle of mice or human patients with diverse muscle pathologies and identified LMCD1 as a gene strongly associated with skeletal muscle function. We transiently expressed or silenced LMCD1 in mouse gastrocnemius muscle or in mouse primary muscle cells and determined muscle/cell size, targeted gene expression, kinase activity with kinase arrays, protein immunoblotting, and protein synthesis levels. To evaluate force, calcium handling, and fatigue, we transduced the flexor digitorum brevis muscle with a LMCD1-expressing adenovirus and measured specific force and sarcoplasmic reticulum Ca2+ release in individual fibers. Finally, to explore the relationship between LMCD1 and calcineurin, we ectopically expressed Lmcd1 in the gastrocnemius muscle and treated those mice with cyclosporine A (calcineurin inhibitor). In addition, we used a luciferase reporter construct containing the myoregulin gene promoter to confirm the role of a LMCD1-calcineurin-myoregulin axis in skeletal muscle mass control and calcium handling.

Results
Here, we identify LIM and cysteine-rich domains 1 (LMCD1) as a positive regulator of muscle mass, that increases muscle protein synthesis and fiber size. LMCD1 expression in vivo was sufficient to increase specific force with lower requirement for calcium handling and to reduce muscle fatigue. Conversely, silencing LMCD1 expression impairs calcium handling and force, and induces muscle fatigue without overt atrophy. The actions of LMCD1 were dependent on calcineurin, as its inhibition using cyclosporine A reverted the observed hypertrophic phenotype. Finally, we determined that LMCD1 represses the expression of myoregulin, a known negative regulator of muscle performance. Interestingly, we observed that skeletal muscle LMCD1 expression is reduced in patients with skeletal muscle disease.

Conclusions
Our gain- and loss-of-function studies show that LMCD1 controls protein synthesis, muscle fiber size, specific force, Ca2+ handling, and fatigue resistance. This work uncovers a novel role for LMCD1 in the regulation of skeletal muscle mass and function with potential therapeutic implications.
Abiotic environmental factors override phytoplankton succession in shaping both free-living and attached bacterial communities in a highland lake
Authors: Wang Huan , Zhu Rong , Zhang Xiaolin , Li Yun , Ni Leyi , Xie Ping , Shen Hong 
Published: October 2019

Abstract
Bacterial communities are an important part of biological diversity and biogeochemical cycling in aquatic ecosystems. In this study, the relationship amongst the phytoplankton species composition and abiotic environmental factors on seasonal changes in the community composition of free-living and attached bacteria in Lake Erhai were studied. Using Illumina high-throughput sequencing, we found that the impact of environmental factors on both the free-living and attached bacterial community composition was greater than that of the phytoplankton community, amongst which total phosphorus, Secchi disk, water temperature, dissolved oxygen and conductivity strongly influenced bacterial community composition. Microcystis blooms associated with subdominant Psephonema occurred during the summer and autumn, and Fragilaria, Melosira and Mougeotia were found at high densities in the other seasons. Only small numbers of algal species-specific bacteria, including Xanthomonadaceae (Proteobacteria) and Alcaligenaceae (Betaproteobacteria), were tightly coupled to Microcystis and Psephonema during Microcystis blooms. Redundancy analysis showed that although the composition of the bacterial communities was controlled by species composition mediated by changes in phytoplankton communities and abiotic environmental factors, the impact of the abiotic environment on both free-living and attached bacterial community compositions were greater than the impact of the phytoplankton community. These results suggest that the species composition of both free-living and attached bacterial communities are affected by abiotic environmental factors, even when under strong control by biotic factors, particularly dominant genera of Microcystis and Psephonema during algal blooms.
Effect of the inoculation of plant growth-promoting rhizobacteria on the photosynthetic characteristics of Sambucus williamsii Hance container seedlings under drought stress
Authors: Liu Fangchun , Ma Hailin , Peng Lin , Du Zhenyu , Ma Bingyao , Liu Xinghong 
Published: October 2019

Abstract
Plant growth-promoting rhizobacteria (PGPR) are beneficial bacteria that survive within the range of plant rhizosphere and can promote plant growth. The effects of PGPR in promoting plant growth, activating soil nutrients, reducing fertilizer application, and improving the resistance of plant inducible system have been widely investigated. However, few studies have investigated PGPR as elicitors of tolerance to abiotic stresses, especially drought stress. In this study, the effects of Acinetobacter calcoaceticus X128 on the photosynthetic rate (Pn), stomatal conductance (Gs), intracellular CO2 concentration (Ci), and total chlorophyll content [Chl(a+b)] of Sambucus williamsii Hance seedling leaves under moderate drought stress and drought-rewatering conditions were determined. Compared with those of uninoculated seedlings, the average Pn values during the entire drought stress of inoculated seedlings increased by 12.99%. As the drought duration was lengthened, Ci of uninoculated leaves continued to increase after rapidly declining, whereas Gs continuously decreased. Furthermore, their photosynthetic properties were simultaneously restricted by stomatal and non-stomatal factors. After X128 inoculation, Ci and Gs of S. williamsii Hance leaves continued to decrease, and their photosynthetic properties were mainly restricted by stomatal factors. At the end of the drought stress, water stress reduced [Chl(a + b)] of S. williamsii Hance leaves by 13.49%. However, X128 inoculation decreased this deficit to only 7.39%. After water supply was recovered, Pn, Gs, and [Chl(a+b)] in uninoculated leaves were reduced by 14.23%, 12.02%, and 5.86%, respectively, relative to those under well-watered conditions. However, Ci increased by 6.48%. Compared with those of uninoculated seedlings, Pn, Gs, and [Chl(a+b)] in X128-inoculated seedlings were increased by 9.83%, 9.30%, and 6.85%, respectively. Therefore, the inoculation of X128 under arid environments can mitigate the reduction of chlorophyll, delay the restriction caused by non-stomatal factors to Pn in plant leaves under water stress, and can be more conducive to the recovery of photosynthetic functions of leaves after water supply is recovered.
Application of biological and single-strand conformation polymorphism assays for characterizing potential mild isolates of Citrus tristeza virus for cross protection
Authors: Atta Sagheer , Umar Ummad ud din , Bashir Muhammad Amjad , Hannan Abdul , Rehman Ateeq ur , Naqvi Syed Atif Hasan , Zhou Changyong 
Published: October 2019

AbstractCitrus tristeza virus(CTV) by killing millions of citrus cultivars grown on sour orange rootstock worldwide has become one of the most dangerous viral pathogen. Characterization of 12 CTV isolates was analyzed by biological indexing. Infected samples of citrus were collected from citrus growing areas of Pakistan and CTV was detected by symptoms on indicator plants and confirmed by direct tissue blot immunoassay (DTBIA). CTV positive samples were graft inoculated on six biological indicator hosts in the study. A standardized protocol was deployed to study biological characteristics of these isolates. All biological indicators induced mild and from mild to moderate reactions against all of the CTV isolates tested. About two isolates produced stem-pitting symptoms from moderate to severe on Mexican lime. CTV strains were further characterized and confirmed by the analysis of p25 gene of CTV isolates using single-strand conformation polymorphism (SSCP) assay. SSCP analysis revealed that most isolates confined only one predominant sequence variant. SSCP profiles of PCR amplified products from CTV isolates showed bands patterns corresponding to mild and sever strain. Three isolates (4MF, 8KBS and 10GS) from different regions and cultivars were identified as potential source of mild strains for cross protection. These results are the best base for mild strain cross protection (MSCP) in the country.
Assessing peer review pattern and the effect of face-to-face and mobile-mediated modes on students’ academic writing development
Authors: Farahani Ali Akbar Khomeijani , Nemati Majid , Nazari Montazer Mostafa 
Published: October 2019

AbstractThis study examines the distribution of peer review in face-to-face and mobile-mediated peer review groups and their effects on students’ revision skills and academic writing development. Seventy-two first-year English for academic purposes (EAP) students participated in an 18-session IELTS academic writing course in a Canadian university the mobile-mediated peer review group (MMPR) used Telegram to exchange peer comments synchronously, while the face-to-face peer review group (FFPR) did peer review in the classroom. An adapted analytic scheme (Journal of English for Academic Purposes, 2, 193–227, 2003) and the IELTS academic writing assessment criteria were used to conceptualize the peer comments in terms of frequency, area, type, nature, and IELTS assessment categories. Results indicated that the total number of comments, the percentage of revision-oriented comments and actual revisions made by the MMPR group were statistically more significant than those by the FFPR group. Furthermore, the MMPR group made more local revision-oriented comments than that of FFPR. However, the revision-oriented suggestion in local areas was the most distributed type of comment made by both groups. Regarding the IELTS assessment criteria, the FFPR group made more comments on task achievement and coherence and cohesion, whereas the comments made by the MMPR group targeted more lexical resources, and grammatical range and accuracy. In addition, the results showed that both MMPR and FFPR groups developed their IELTS academic writing skills while the MMPR mode of collaboration outperformed the FFPR.
Mendel’s pea crosses: varieties, traits and statistics
Authors: Ellis T. H. Noel , Hofer Julie M. I. , Swain Martin T. , van Dijk Peter J. 
Published: October 2019

AbstractA controversy arose over Mendel’s pea crossing experiments after the statistician R.A. Fisher proposed how these may have been performed and criticised Mendel’s interpretation of his data. Here we re-examine Mendel’s experiments and investigate Fisher’s statistical criticisms of bias. We describe pea varieties available in Mendel’s time and show that these could readily provide all the material Mendel needed for his experiments; the characters he chose to follow were clearly described in catalogues at the time. The combination of character states available in these varieties, together with Eichling’s report of crosses Mendel performed, suggest that two of his F3 progeny test experiments may have involved the same F2 population, and therefore that these data should not be treated as independent variables in statistical analysis of Mendel’s data. A comprehensive re-examination of Mendel’s segregation ratios does not support previous suggestions that they differ remarkably from expectation. The χ2values for his segregation ratios sum to a value close to the expectation and there is no deficiency of extreme segregation ratios. Overall the χ values for Mendel’s segregation ratios deviate slightly from the standard normal distribution; this is probably because of the variance associated with phenotypic rather than genotypic ratios and because Mendel excluded some data sets with small numbers of progeny, where he noted the ratios “deviate not insignificantly” from expectation.
Ultra-high performance liquid chromatography-MS/MS (UHPLC-MS/MS) in practice: analysis of drugs and pharmaceutical formulations
Authors: Rathod Ravsaheb H. , Chaudhari Suraj R. , Patil Amod S. , Shirkhedkar Atul A. 
Published: October 2019

Abstract

Background
UHPLC-MS/MS is connected in various research facilities for the qualitative and quantitative investigation of a pharmaceutical substance, pharmaceutical items, and biological specimen.

Main body
The commence review article is an endeavor to offer pervasive awareness around assorted aspects and details about the UHPLC-MS/MS and related techniques with the aim on practice to an estimation of medicinal active agents in the last 10 years. The article also focused on isolation, separation, and characterization of present impurity in drug and biological samples.

Conclusion
Review article compiles a general overview of medicinally important drugs and their analysis with UHPLC-MS/MS. It gives fundamental thought regarding applications of UHPLC-MS/MS for the study on safety limit. The summary of developed UHPLC-MS/MS methods gives a contribution to the future trend and limitations in this area of research.
Frequency-dependent AVO analysis using the scattering response of a layered reservoir
Authors: Kumar Dhananjay , Zhao Zeyu , Foster Douglas J. , Dralus Danica , Sen Mrinal K. 
Published: October 2019

Sensitivity of reservoir properties to broadband seismic amplitudes can be weak, which makes interpretation ambiguous. Examples of challenging interpretation scenarios include distinguishing blocky reservoirs from fining sequences, low gas saturation from high gas saturation, and variable reservoir quality. Some of these rock and fluid changes might indicate stronger sensitivity to amplitudes over narrow frequency bands, which is a characteristic of frequency-dependent amplitude variation with offset (FAVO). We have developed a FAVO model for reservoir characterization, following a seismic scattering phenomenon through a set of isotropic elastic layers. The frequency dependency in our model comes from the time delays due to wave propagation within layers. The FAVO modeled response is a complex-valued amplitude varying with angle and frequency, and it is a function of the seismic velocities and thicknesses of individual layers, along with the conventional AVO response at all interfaces. Our FAVO seismic analysis consists of two main steps: (1) forward modeling using well logs to understand rock and fluid sensitivity to amplitudes to identify tuning frequencies with maximum amplitude excursions and (2) seismic analysis at tuning frequencies. With well-log models, we observed that the frequency-dependent tuning response is primarily dependent on the lithology stacking pattern of a reservoir; in the cases studied, the fluid and reservoir quality have secondary effects on the frequency dependence of the amplitudes. We evaluate synthetic models and field data from the Columbus Basin, Trinidad, to illustrate our frequency-dependent seismic analysis methods. For one of the sandstone reservoirs, a frequency-dependent attribute indicates better spatial resolution of the anomaly than a conventional amplitude extraction. FAVO attributes are complementary to conventional AVO attributes.
Validation of a 3D CBCT-based protocol for the follow-up of mandibular condyle remodeling
Authors: Verhelst Pieter-Jan , Shaheen Eman , de Faria Vasconcelos Karla , Van der Cruyssen Fréderic , Shujaat Sohaib , Coudyzer Walter , Salmon Benjamin , Swennen Gwen , Politis Constantinus , Jacobs Reinhilde 
Published: November 2019

Objectives: Three-dimensional models of mandibular condyles provide a way for condylar remodeling follow-up. The overall aim was to develop and validate a user-friendly workflow for cone beam CT (CBCT)-based semi-automatic condylar registration and segmentation. Methods: A rigid voxel-based registration (VBR) technique for registration of two post-operative CBCT-scans was tested. Two modified mandibular rami, with or without gonial angle, were investigated as the volume of interest for registration. Inter- and intraoperator reproducibility of this technique was tested on 10 mandibular rami of orthognathic patients by means of intraclass correlation coefficients (ICC’s) and descriptive statistics of the transformation values from the VBR. The difference in reproducibility between the two modified rami was evaluated using a paired t-test (p < 0.05). For the segmentation, eight fresh frozen cadaver heads were scanned with CBCT and micro-CT. These data were used to test the inter- and intraoperator reproducibility (ICC’s) and accuracy (Bland–Altman plot) of a newly designed workflow based on semi-automated contour enhancement. Results: Excellent ICC’s (0.94–0.99) were obtained for the voxel-based registration technique using both modified rami. If the gonial angle was not included in the volume of interest, there was a trend of increased operator error suggested by significant higher interoperator differences in translation values (p = 0,0036). The segmentation workflow proved to be highly reproducible with excellent ICC’s (0.99), low absolute mean volume differences between operators (23.19 mm3), within operators (28.93 mm3) and low surface distances between models of different operators (<0.20 mm). Regarding the accuracy, CBCT-models slightly overestimate the condylar volume compared to micro-CT. Conclusions: This study provides a validated user-friendly and reproducible method of creating three-dimensional-surface models of mandibular condyles out of longitudinal CBCT-scans.