bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nadir experiences and ego-integrity: types of narrative enactment
Authors: Chrz Vladimir , Dubovská Eva , Poláčková Šolcová Iva 
Published: March 2020

AbstractComing to terms with negative life experiences is a way of reaching ego-integrity in mature age, a development task belonging to the last phase of Erikson's theory of psycho-social development. This study explores fundamental tensions between negative life experiences and ego-integrity through the lens of narrative enactment,i.e.by looking at how nadir experiences are constructed in the narratives of study participants. From 166 potential participants, we selected life-story narratives of 42 highly ego-integrated older adults based on a self-rated ego-integrity scale. Data were analysed using the narrative approach, where ageing is perceived as autobiographical work and narrativity is viewed as enactment of the constitutive event of ‘trouble’. Our results suggest a subtle classification of five distinct types of narrative enactment: ‘it turned out well’, ‘I managed to cope with it’, ‘validation with respect to future development’, ‘acquisition of a new attitude’ and, finally, ‘justification of a hard decision’. Each of these enactment types is defined by a particular configuration of narrative categories: agency in a negative event, agency in a positive resolution, reflexivity and construction of moral identity. Our results highlight the meaning of processing and reconstruction of negative life experiences and the various narrative pathways this process can follow in highly ego-integrated older adults.
Always alone? Network transitions among detached older Europeans and their effects
Authors: Litwin Howard , Levinsky Michal 
Published: March 2020

AbstractThe present study investigated the changes that occurred within the interpersonal environments of a sample of socially isolated older Europeans after four years. Based upon data from the Survey of Health, Ageing and Retirement in Europe (SHARE), the inquiry looked at 834 older people who were socially detached at baseline, at follow-up or at both time-points. The analysis traced changes in relation to the social network types that they acquired or left. The findings indicate that respondents more frequently moved from no-network status at baseline to close family-based social network types at follow-up than to other network types. In comparison, the loss of a network at follow-up was seen to originate from close networks and from other network types alike. Multivariate analysis revealed that those who moved from socially detached status to close-family networks had fewer depressive symptoms compared to those who moved to other network types. However, the move from a close-family network to the no-network status was about as depressing as was being chronically isolated. The effects of network transitions were more varied in relation to the life satisfaction outcome. In sum, the findings suggest that a transition out of social isolation in late life is possible and it may be beneficial as well.
Evolution of Elastic and Mechanical Properties During Fault Shear: The Roles of Clay Content, Fabric Development, and Porosity
Authors: Kenigsberg Abby R. , Rivière Jacques , Marone Chris , Saffer Demian M. 
Published: March 2020

AbstractPhyllosilicates weaken faults due to the formation of shear fabrics. Although the impacts of clay abundance and fabric on frictional strength, sliding stability, and porosity of faults are well studied, their influence on elastic properties is less known, though they are key factors for fault stiffness. We document the role that fabric and consolidation play in elastic properties and show that smectite content is the most important factor determining whether fabric or porosity controls the elastic response of faults. We conducted a suite of shear experiments on synthetic smectite‐quartz fault gouges (10–100 wt% smectite) and sediment incoming to the Sumatra subduction zone. We monitored Vp, Vs, friction, porosity, shear and bulk moduli. We find that mechanical and elastic properties for gouges with abundant smectite are almost entirely controlled by fabric formation (decreasing mechanical and elastic properties with shear). Though fabrics control the elastic response of smectite‐poor gouges over intermediate shear strains, porosity is the primary control throughout the majority of shearing. Elastic properties vary systematically with smectite content: High smectite gouges have values of Vp ~ 1,300–1,800 m/s, Vs ~ 900–1,100 m/s, K ~ 1–4 GPa, and G ~ 1–2 GPa, and low smectite gouges have values of Vp ~ 2,300–2,500 m/s, Vs ~ 1,200–1,300 m/s, K ~ 5–8 GPa, and G ~ 2.5–3 GPa. We find that, even in smectite‐poor gouges, shear fabric also affects stiffness and elastic moduli, implying that while smectite abundance plays a clear role in controlling gouge properties, other fine‐grained and platy clay minerals may produce similar behavior through their control on the development of fabrics and thin shear surfaces.
Depth‐Resolved Photochemical Lability of Dissolved Organic Matter in the Western Tropical Pacific Ocean
Authors: Yang Fangming , Song Guisheng , Massicotte Philippe , Wei Hao , Xie Huixiang 
Published: March 2020

AbstractWater samples collected from various depths of the offshore South China and Philippine Seas were exposed to solar‐simulated radiation. Photomineralization of dissolved organic carbon (DOC) and photobleaching of chromophoric dissolved organic matter (CDOM) and its humic‐like fluorescent constituent (FDOM) were observed in all samples. Protein‐like FDOM was, however, either photo‐decomposed or photo‐produced, depending on the sample's depth. The photobleaching of CDOM and humic‐like FDOM was much faster in deep than in shallow water samples while photomineralization displayed a weaker vertical zonation. Prior‐irradiated deep water inoculated with surface‐water bacteria showed enhanced microbial DOC removal but CDOM production. Results from this study suggest that deep‐ocean CDOM and FDOM can barely survive photobleaching during one ocean mixing cycle, but photochemical turnover of the bio‐refractory deep DOC is considerably longer than its average radiocarbon age. Coupled photochemical‐microbial processes can not only remove part of the bio‐refractory deep DOM but also regenerate part of it during ocean overturning circulation.
An epidermal sEMG tattoo-like patch as a new human–machine interface for patients with loss of voice
Authors: Liu Huicong , Dong Wei , Li Yunfei , Li Fanqi , Geng Jiangjun , Zhu Minglu , Chen Tao , Zhang Hongmiao , Sun Lining , Lee Chengkuo 
Published: March 2020

AbstractThroat cancer treatment involves surgical removal of the tumor, leaving patients with facial disfigurement as well as temporary or permanent loss of voice. Surface electromyography (sEMG) generated from the jaw contains lots of voice information. However, it is difficult to record because of not only the weakness of the signals but also the steep skin curvature. This paper demonstrates the design of an imperceptible, flexible epidermal sEMG tattoo-like patch with the thickness of less than 10 μm and peeling strength of larger than 1 N cm−1 that exhibits large adhesiveness to complex biological surfaces and is thus capable of sEMG recording for silent speech recognition. When a tester speaks silently, the patch shows excellent performance in recording the sEMG signals from three muscle channels and recognizing those frequently used instructions with high accuracy by using the wavelet decomposition and pattern recognization. The average accuracy of action instructions can reach up to 89.04%, and the average accuracy of emotion instructions is as high as 92.33%. To demonstrate the functionality of tattoo-like patches as a new human–machine interface (HMI) for patients with loss of voice, the intelligent silent speech recognition, voice synthesis, and virtual interaction have been implemented, which are of great importance in helping these patients communicate with people and make life more enjoyable.
Transcription co-activator P300 activates Elk1-aPKC-ι signaling mediated epithelial-to-mesenchymal transition and malignancy in hepatocellular carcinoma
Authors: Ma Chaoqun , Huang Shuhong , Xu Lei , Tian Li , Yang Yan , Wang Jianming 
Published: March 2020

AbstractEpithelial-to-mesenchymal transition (EMT) plays an important role in invasion and metastasis of hepatocellular carcinoma (HCC). Our previous study found that atypical protein kinase C-ι (aPKC-ι) promoted the EMT process in HCC. However, how the aPKC-ι signaling pathway is regulated in HCC has not been elucidated. In this study, vector transfection was utilized to study the invasion of HCC cells, and the mechanism between P300 and aPKC-ι signaling pathways in regulating the EMT process of HCC was further elucidated in vitro and in vivo. We found both P300 and aPKC-ι were highly expressed in HCC and they were correlated with tumor progression and poor survival in HCC patients. P300 knockdown inhibited EMT, invasion and other malignant events of HCC cells but promoted cell apoptosis and cycle arrest. However, the effects mediated by P300 knockdown were abolished by aPKC-ι overexpression. Further studies showed that P300 upregulates aPKC-ι expression through increasing the transcription of Elk1, a transcriptional activator of aPKC-ι, and stabilizing Elk1 protein and its phosphorylation. In conclusion, our work uncovered the molecular mechanism by which oncogenic aPKC-ι is upregulated in HCC and suggests that P300, like aPKC-ι, may be used as a prognostic biomarker and therapeutic target in patients with HCC.
Intrinsic origin of interfacial second-order magnetic anisotropy in ferromagnet/normal metal heterostructures
Authors: Gweon Hyung Keun , Park Hyeon-Jong , Kim Kyoung-Whan , Lee Kyung-Jin , Lim Sang Ho 
Published: March 2020

AbstractInterfacial perpendicular magnetic anisotropy, which is characterized by first-order (K1) and second-order (K2) anisotropy, is the core phenomenon for nonvolatile magnetic devices. A sizable K2 satisfying a specific condition stabilizes the easy-cone state, where equilibrium magnetization forms at an angle from the film normal. The easy-cone state offers intriguing possibilities for advanced spintronic devices and unique spin textures, such as spin superfluids and easy-cone domain walls. Experimental realization of the easy-cone state requires understanding the origin of K2, thereby enhancing K2. However, the previously proposed origins of K2 cannot fully account for the experimental results. Here, we experimentally show that K2 scales almost linearly with the work function difference between the Co and X layers in Pt/Co/X heterostructures (X = Pd, Cu, Pt, Mo, Ru, W, and Ta), suggesting the central role of the inversion asymmetry in K2. Our result provides a guideline for enhancing K2 and realizing magnetic applications based on the easy-cone state.
An analytical theory of balanced cellular growth
Authors: Dourado Hugo , Lercher Martin J. 
Published: March 2020

AbstractThe biological fitness of microbes is largely determined by the rate with which they replicate their biomass composition. Mathematical models that maximize this balanced growth rate while accounting for mass conservation, reaction kinetics, and limits on dry mass per volume are inevitably non-linear. Here, we develop a general theory for such models, termed Growth Balance Analysis (GBA), which provides explicit expressions for protein concentrations, fluxes, and growth rates. These variables are functions of the concentrations of cellular components, for which we calculate marginal fitness costs and benefits that are related to metabolic control coefficients. At maximal growth rate, the net benefits of all concentrations are equal. Based solely on physicochemical constraints, GBA unveils fundamental quantitative principles of cellular resource allocation and growth; it accurately predicts the relationship between growth rates and ribosome concentrations inE. coliand yeast and between growth rate and dry mass density inE. coli.
Internal control beliefs shape positive affect and associated neural dynamics during outcome valuation
Authors: Stolz David S. , Müller-Pinzler Laura , Krach Sören , Paulus Frieder M. 
Published: March 2020

AbstractExperiencing events as controllable is essential for human well-being. Based on classic psychological theory, we test how internal control beliefs impact the affective valuation of task outcomes, neural dynamics and ensuing behavioral preferences. In three consecutive studies we show that dynamics in positive affect increase, with a qualitative shift towards self-evaluative pride, when agents believe they caused a given outcome. We demonstrate that these outcomes engage brain networks processing self-referential information in the cortical midline. Here, activity in the ventromedial prefrontal cortex tracks outcome valence regarding both success as well as internal control, and covaries with positive affect in response to outcomes. These affective dynamics also relate to increased functional coupling between the ventral striatum and cortical midline structures. Finally, we show that pride predicts preferences for control, even at monetary costs. Our investigations extend recent models of positive affect and well-being, and emphasize that control beliefs drive intrinsic motivation.
Virtual-freezing fluorescence imaging flow cytometry
Authors: Mikami Hideharu , Kawaguchi Makoto , Huang Chun-Jung , Matsumura Hiroki , Sugimura Takeaki , Huang Kangrui , Lei Cheng , Ueno Shunnosuke , Miura Taichi , Ito Takuro , Nagasawa Kazumichi , Maeno Takanori , Watarai Hiroshi , Yamagishi Mai , Uemura Sotaro , Ohnuki Shinsuke , Ohya Yoshikazu , Kurokawa Hiromi , Matsusaka Satoshi , Sun Chia-Wei , Ozeki Yasuyuki , Goda Keisuke 
Published: March 2020

AbstractBy virtue of the combined merits of flow cytometry and fluorescence microscopy, imaging flow cytometry (IFC) has become an established tool for cell analysis in diverse biomedical fields such as cancer biology, microbiology, immunology, hematology, and stem cell biology. However, the performance and utility of IFC are severely limited by the fundamental trade-off between throughput, sensitivity, and spatial resolution. Here we present an optomechanical imaging method that overcomes the trade-off by virtually freezing the motion of flowing cells on the image sensor to effectively achieve 1000 times longer exposure time for microscopy-grade fluorescence image acquisition. Consequently, it enables high-throughput IFC of single cells at >10,000 cells s−1 without sacrificing sensitivity and spatial resolution. The availability of numerous information-rich fluorescence cell images allows high-dimensional statistical analysis and accurate classification with deep learning, as evidenced by our demonstration of unique applications in hematology and microbiology.
Maltotriose-based probes for fluorescence and photoacoustic imaging of bacterial infections
Authors: Zlitni Aimen , Gowrishankar Gayatri , Steinberg Idan , Haywood Tom , Sam Gambhir Sanjiv 
Published: March 2020

AbstractCurrently, there are no non-invasive tools to accurately diagnose wound and surgical site infections before they become systemic or cause significant anatomical damage. Fluorescence and photoacoustic imaging are cost-effective imaging modalities that can be used to noninvasively diagnose bacterial infections when paired with a molecularly targeted infection imaging agent. Here, we develop a fluorescent derivative of maltotriose (Cy7-1-maltotriose), which is shown to be taken up in a variety of gram-positive and gram-negative bacterial strains in vitro. In vivo fluorescence and photoacoustic imaging studies highlight the ability of this probe to detect infection, assess infection burden, and visualize the effectiveness of antibiotic treatment in E. coli-induced myositis and a clinically relevant S. aureus wound infection murine model. In addition, we show that maltotriose is an ideal scaffold for infection imaging agents encompassing better pharmacokinetic properties and in vivo stability than other maltodextrins (e.g. maltohexose).
Olfactory memory representations are stored in the anterior olfactory nucleus
Authors: Aqrabawi Afif J. , Kim Jun Chul 
Published: March 2020

AbstractThe anterior olfactory nucleus (AON) is the initial recipient of odour information from the olfactory bulb, and the target of dense innervation conveying spatiotemporal cues from the hippocampus. We hypothesized that the AON detects the coincidence of these inputs, generating patterns of activity reflective of episodic odour engrams. Using activity-dependent tagging combined with neural manipulation techniques, we reveal that contextually-relevant odour engrams are stored within the AON and that their activity is necessary and sufficient for the behavioural expression of odour memory. Our findings offer a new model for studying the mechanisms underlying memory representations.
Intensive care for human hearts in pluripotent stem cell models
Authors: Golforoush Pelin , Schneider Michael D. 
Published: March 2020

AbstractSuccessful drug discovery is ultimately contingent on the availability of workable, relevant, predictive model systems. Conversely, for cardiac muscle, the lack of human preclinical models to inform target validation and compound development has likely contributed to the perennial problem of clinical trial failures, despite encouraging non-human results. By contrast, human cardiomyocytes produced from pluripotent stem cell models have recently been applied to safety pharmacology, phenotypic screening, target validation and high-throughput assays, facilitating cardiac drug discovery. Here, we review the impact of human pluripotent stem cell models in cardiac drug discovery, discussing the range of applications, readouts, and disease models employed, along with the challenges and prospects to advance this fruitful mode of research further.
Replacing fishmeal with plant protein in Atlantic salmon (Salmo salar) diets by supplementation with fish protein hydrolysate
Authors: Egerton S. , Wan A. , Murphy K. , Collins F. , Ahern G. , Sugrue I. , Busca K. , Egan F. , Muller N. , Whooley J. , McGinnity P. , Culloty S. , Ross R. P. , Stanton C. 
Published: March 2020

AbstractThe effects of feeding an 80% plant protein diet, with and without fish protein hydrolysate (FPH) supplementation, on the growth and gut health of Atlantic salmon were investigated. Fish were fed either (A) a control diet containing 35% fishmeal, (B) an 80% plant protein diet with 15% fishmeal, (C) an 80% plant protein diet with 5% fishmeal and 10% partly hydrolysed protein, or (D) an 80% plant protein diet with 5% fishmeal and 10% soluble protein hydrolysate. Fish on the 80% plant- 15% fishmeal diet were significantly smaller than fish in the other dietary groups. However, partly-hydrolysed protein supplementation allowed fish to grow as well as fish fed the control 35% fishmeal diet. Fish fed the FPH diets (diets C and D) had significantly higher levels of amino acids in their blood, including 48% and 27% more branched chain amino acids compared to fish on the 35% fishmeal diet, respectively. Plant protein significantly altered gut microbial composition, significantly decreasing α-diversity. Spirochaetes and the familiesMoritellaceae, Psychromonadaceae, HelicobacteraceaeandBacteroidaceaewere all found at significantly lower abundances in the groups fed 80% plant protein diets compared to the control fishmeal diet.
Suppression of antibiotic resistance evolution by single-gene deletion
Authors: Horinouchi Takaaki , Maeda Tomoya , Kotani Hazuki , Furusawa Chikara 
Published: March 2020

AbstractAntibiotic treatment generally results in the selection of resistant bacterial strains, and the dynamics of resistance evolution is dependent on complex interactions between cellular components. To better characterize the mechanisms of antibiotic resistance and evaluate its dependence on gene regulatory networks, we performed systematic laboratory evolution of Escherichia coli strains with single-gene deletions of 173 transcription factors under three different antibiotics. This resulted in the identification of several genes whose deletion significantly suppressed resistance evolution, including arcA and gutM. Analysis of double-gene deletion strains suggested that the suppression of resistance evolution caused by arcA and gutM deletion was not caused by epistatic interactions with mutations known to confer drug resistance. These results provide a methodological basis for combinatorial drug treatments that may help to suppress the emergence of resistant pathogens by inhibiting resistance evolution.
The Role of Transthyretin in Oligodendrocyte Development
Authors: Alshehri Bandar , Pagnin Maurice , Lee Jae Young , Petratos Steven , Richardson Samantha J. 
Published: March 2020

AbstractTransthyretin (TTR) is a protein that binds and distributes thyroid hormones (THs) in blood and cerebrospinal fluid. Previously, two reports identified TTR null mice as hypothyroid in the central nervous system (CNS). This prompted our investigations into developmentally regulated TH-dependent processes in brains of wildtype and TTR null mice. Despite logical expectations of a hypomyelinating phenotype in the CNS of TTR null mice, we observed a hypermyelination phenotype, synchronous with an increase in the density of oligodendrocytes in the corpus callosum and anterior commissure of TTR null mice during postnatal development. Furthermore, absence of TTR enhanced proliferation and migration of OPCs with decreased apoptosis. Neural stem cells (NSCs) isolated from the subventricular zone of TTR null mice at P21 revealed that the absence of TTR promoted NSC differentiation toward a glial lineage. Importantly, we identified TTR synthesis in OPCs, suggestive of an alternate biological function in these cells that may extend beyond an extracellular TH-distributor protein. The hypermyelination mechanism may involve increased pAKT (involved in oligodendrocyte maturation) in TTR null mice. Elucidating the regulatory role of TTR in NSC and OPC biology could lead to potential therapeutic strategies for the treatment of acquired demyelinating diseases.
The interplay between bone healing and remodeling around dental implants
Authors: Irandoust Soroush , Müftü Sinan 
Published: March 2020

AbstractLong-term bone healing/adaptation after a dental implant treatment starts with diffusion of mesenchymal stem cells to the wounded region and their subsequent differentiation. The healing phase is followed by the bone-remodeling phase. In this work, a mechano-regulatory cellular differentiation model was used to simulate tissue healing around an immediately loaded dental implant. All tissue types were modeled as poroelastic in the healing phase. Material properties of the healing region were updated after each loading cycle for 30 cycles (days). The tissue distribution in the healed state was then used as the initial condition for the remodeling phase during which regions healed into bone adapt their apparent density with respect to a homeostatic remodeling stimulus. The short- (bone healing) and long-term (bone remodeling) effects of initial implant micromotion during the healing phase were studied. Development of soft tissue was observed both in the coronal region due to high fluid velocity, and on the vertical sides of the healing-gap due to high shear stress. In cases with small implant micromotion, tissue between the implant threads differentiated into bone during the healing phase but resorbed during remodeling. In cases with large implant micromotion, higher percentage of the healing region differentiated into soft tissue resulting in smaller volume of bone tissue available for remodeling. However, the remaining bone region developed higher density bone tissue. It was concluded that an optimal range of initial implant micromotion could be designed for a specific patient in order to achieve the desired long-term functional properties.