bluuebunny/crossref_metadata_2025_split huggingface dataset:
miR-219a-5p enhances the radiosensitivity of non-small cell lung cancer cells through targeting CD164
Authors: Wei Tao , Cheng Shan , Fu Xiao Na , Feng Lian Jie 
Published: May 2020

Abstract
Lung cancer is one of the leading causes of cancer-associated mortality. Non-small cell lung carcinoma (NSCLC) accounts for 70–85% of the total cases of lung cancer. Radioresistance frequently develops in NSCLC in the middle and later stages of radiotherapy. We investigated the role of miR-219a-5p in radioresistance of NSCLC. miR-219a-5p expression in serum and lung tissue of lung cancer patients was lower than that in control. Compared with radiosensitive (RS) NSCLC patients, miR-219a-5p expression was decreased in serum and lung tissue in radioresistant patients. miR-219a-5p expression level was negatively associated with radioresistance in NSCLC cell lines. Up-regulation of miR-219a-5p increased radiosensitivity in radioresistant NSCLC cells in vitro and in vivo. Down-regulation of miR-219a-5p decreased radiosensitivity in radiosensitive A549 and H358 cells. miR-219a-5p could directly bind in the 3′UTR of CD164 and negatively regulated CD164 expression. CD164 expression was higher in radioresistant NSCLC tissues than RS tissues. Up-regulation of CD164 significantly inhibited miR-219a-5p-induced regulation of RS in radioresistant A549 and H358 cells. Down-regulation of CD164 significantly inhibited the effect of anti-miR-219a-5p on radiosensitive A549 and H358 cells. miR-219a-5p or down-regulation of CD164 could increase apoptosis and γ-H2A histone family member X (γ-H2AX) expression in radioresistant cells in vitro and in vivo. Up-regulation of CD164 could inhibit the effect of miR-219a-5p on apoptosis and γ-H2AX expression. Our results indicated that miR-219a-5p could inhibit CD164, promote DNA damage and apoptosis and enhance irradiation-induced cytotoxicity. The data highlight miR-219a-5p/CD164 pathway in the regulation of radiosensitivity in NSCLC and provide novel targets for potential intervention.
Fine-scale visualizing the hierarchical structure of mouse biliary tree with fluorescence microscopy method
Authors: Chen Yuwei , Bai Lin , Zhou Yongjie , Zhang Xiaoyun , Zhang Jie , Shi Yujun 
Published: May 2020

Abstract
The liver is a vital organ and the hepatic lobule serves as the most basic structural and functional unit which is mainly assembled with parenchymal cells including hepatocytes and biliary epithelial cells. The continuous tubular arrangement of biliary cells which constitutes the biliary tracts is critical for liver function, however, the biliary tracts are often disrupted in many liver diseases such as cirrhosis and some congenital disorders. Visualization of the biliary tracts in fine-scale and three-dimension will help to understanding the structure basis of these liver diseases. In the present study, we established several biliary tract injury mouse models by diet feeding, surgery or genetic modification. The cytoplasm and nuclei of the parenchymal cells were marked by active uptake of fluorescent dyes Rhodamine B (red) and Hoechst (blue), respectively. After the removal of liver en bloc, the biliary tracts were retrogradely perfused with green fluorescent dye, fluorescein isothiocyanate (FITC). The liver was then observed under confocal microscopy. The fine-scale and three-dimensional (3D) structure of the whole biliary tree, particularly the network of the end-terminal bile canaliculi and neighboring hepatocytes were clearly visualized. The biliary tracts displayed clear distinct characteristics in normal liver and diseased liver models. Taken together, we have developed a simple and repeatable imaging method to visualize the fine-scale and hierarchical architecture of the biliary tracts spreading in the mouse liver.
In Vitro and In Vivo Metabolic Activation of Obacunone, A Bioactive and Potentially Hepatotoxic Constituent of Dictamni Cortex
Authors: Lang Xiuzhuang , Zhang Xiangmei , Wang Daoquan , Zhou Weiqing 
Published: May 2020

AbstractObacunone is one of the major bioactive constituents from Dictamni cortex, a traditional Chinese medicine widely used in China. Oral administration of obacunone or Dictamni cortex extract has been shown to cause liver injury in rats. Given that obacunone contains a furan ring, which is a structural alert, metabolic activation might be responsible for obacunone-induced liver injury. In this study, bioactivation pathways of obacunone in rat and human liver microsomes were investigated. Obacunone was first metabolized into cis-butene-1,4-dial, and then cis-butene-1,4-dial was captured by glutathione, N-acetyl-cysteine, and N-acetyl-lysine in the microsomal incubation system. A total of 13 adducts derived from the reaction of cis-butene-1,4-dial with glutathione and/or N-acetyl-lysine were detected and structurally identified by liquid chromatography coupled to high-resolution tandem mass spectrometry. The major metabolite (M7) was identified to be the cyclic mono-glutathione conjugate of cis-butene-1,4-dial, which was detected in bile and urine of obacunone-treated rats. M9 and M10, obacunone-derived glutathione-cis-butene-1,4-dial-NAL conjugates, were detected in the microsomal incubations of obacunone fortified with glutathione and N-acetyl-lysine as trapping agents. M3 and M4, pyrroline-2-one derivatives, were also detected in microsomal incubations. Further phenotyping studies indicated that ketoconazole showed a strong inhibitory effect on the production of cis-butene-1,4-dial in a concentration-dependent manner. CYP3A4 was demonstrated to be the primary enzyme responsible for the bioactivation of obacunone by using individual recombinant human CYP450 enzymes. The current study provides an overview of CYP450-dominated bioactivation of obacunone and contributes to the understanding of the role of bioactivation in obacunone-induced liver injury.
Sektorenübergreifende Versorgung in der Pädiatrischen
Onkologie: Qualitätssicherung durch neue Vergütungsmodelle
für nicht-vollstationäre Leistungen
Authors: Reinke Evelyn , Jürgens Heribert , Haier Jörg , Froehlich Birgit , Kontny Udo , Rossig Claudia 
Published: May 2020

Zusammenfassung
Hintergrund Die pädiatrisch-onkologische Versorgungsstruktur
nimmt in Deutschland eine Sonderrolle ein. An Krebs erkrankte Kinder und
Jugendliche werden bundesweit einheitlich in Therapiestudien und Registern
sektorenübergreifend an Zentren behandelt, die eine
interdisziplinäre und multiprofessionelle Versorgung sicherstellen
können. Für die Abrechnung der nicht-vollstationären
Leistungen verwenden die Zentren heterogene Abrechnungsmodalitäten, die
den erforderlichen Versorgungsaufwand monetär überwiegend nicht
decken.
Methode Es wurde deutschlandweit eine Umfrage aller Zentren der
Gesellschaft für Pädiatrische Hämatologie und Onkologie
(GPOH) durchgeführt. In der Umfrage wurde standardisiert abgefragt,
über welche Versorgungsarten der nicht-vollstationäre Bereich in
der Kinderonkologie finanziert wird und ob eine Kostendeckung erreicht werden
kann.
Ergebnisse Von 58 kinderonkologischen Zentren beteiligten sich insgesamt
18 (33%) an der Umfrage, darunter 8 (44%)
Universitätsklinika. Die Inanspruchnahme verfügbarer
Abrechnungsarten erwies sich als sehr heterogen. Im Mittel werden
3,33±1,49 Abrechnungsarten pro Zentrum angewandt. 17 der 18
teilnehmenden Zentren gaben an, mit den Erlösen die Kosten für
die nicht-vollstationäre Versorgung nicht decken zu können.
Diskussion und Schlussfolgerung Kinderonkologische Zentren in Deutschland
können eine kostendeckende Versorgung im nicht-vollstationären
Setting nicht erreichen. Für die nicht-vollstationäre Versorgung
an Krebs erkrankter Kinder und Jugendlicher wird eine bundeseinheitliche und
leistungsgerechte Versorgungsform dringend benötigt. Drei verschiedene
Modelle, die einen adäquaten Handlungsrahmen bieten, wurden erarbeitet
und vorgestellt.
Successful Endoscopic Ultrasound Guided Drainage of Large Lesser Sac Abscess Cavity Postgastric Ulcer Sealed-Off Perforation: Novel Approach
Authors: Bhatt Keyur , Mangukiya Dhaval , Desai Pankaj , Parekh Krishna 
Published: May 2020

AbstractEndoscopic ultrasonography (EUS) guided drainage is a beneficial and safe therapeutic tool that is used for the treatment of pancreatic mass and pseudocysts. Currently, the application of EUS-guided drainage is increasing for the treatment of different complications such as intraperitoneal abscess drainage following perforated peptic ulcer (PPU) or pararectal abscess. The intraperitoneal abscess with PPU is traditionally treated with surgical treatment. Herein, we report a lesser invasive drainage technique in the form of EUS-guided drainage for the treatment of intraperitoneal abscess.
PREVALENCE OF OBESITY AMONG HIGH SCHOOL CHILDREN IN DAKSHINA KANNADA AND UDUPI DISTRICTS
Authors: M. Keerthan Kumar , K. Prashanth , Baby Kavya Elizabeth , Rao Kavya Rashmi , B. Kumarkrishna , Hegde Krishnamurthy , Kumar Mrinal , Shetty Manish , N. Navya , P. Kavya C. , G. Sandeep Kumar , R. Rahul 
Published: May 2020

Abstract
Objective: The aim of this project is to find out the prevalence of overweight and obesity in the high school children in selected schools of Mangalore and Manipal and also to study the association of obesity with different factors like Age, Sex, Physical activity etc.
Methods: Cross sectional Study which included 500 participants from 10 selected schools in Udupi and D.K districts. 50 students were rd included from each school on the basis of 1 in 5 choosing every 3rd student. In schools with lesser attendance all were included. Following which they were explained the details about the study. Then their height and weight were recorded using standard measurements and the data was collected using the questionnaire.
Results: The overall prevalence of obesity and overweight was found to be 2.6% and 3.0% respectively. Prevalence was found to be higher in males, those studying in private schools, staying in nuclear family, consuming fried foods-aerated drinks regularly and in those who do not exercise regularly.
Conclusions: Childhood obesity is a major public health problem globally because of changes in lifestyle.
MANAGEMENT OF ACUTE CORNEAL HYDROPS SECONDARY TO KERATOCONUS BY DESCEMETOPEXY USING INTRACAMERAL PERFLUOROPROPANE (C3F8 ) – A CASE REPORT
Authors: Pai Vijay , Shetty Jayaram , Amin Hrishikesh , Bhat S. , Lakshmi Divya 
Published: May 2020

AbstractKeratoconus is a clinical term used to describe bilateral non-inflammatory corneal ectasia in its axial part due to which cornea assumes a conical shape1. The onset of keratoconus is generally at the age of puberty, and progresses over a period of 10-20 years2,3. The treatment of Keratoconus is rarely an emergency, with the exception of corneal hydrops resulting from rupture of the Descemet's membrane. This may be the common mode of presentation in patients with associated developmental delay, probably related to habitual ocular massage4,5.
INTRA-ARTICULAR SYNOVIAL HEMANGIOMA OF KNEE OVERLYING POSTERIOR CRUCIATE LIGAMENT - A CASE REPORT
Authors: S. Dileep K. , Kanakaraddi Sandeep V. , S. Vidyasagar J.V. , S. Radha , Aroor Akshatha Rao 
Published: May 2020

AbstractHemangioma of synovium is a rare benign tumor that can arise from any surface lined by synovium. Intra-articular synovial hemangioma is most common in the knee joint. The diagnosis is difficult as the signs and symptoms are nonspecific. We hereby report a hitherto unreported case of synovial hemangioma of knee overlying posterior cruciate ligament, its MRI findings and discuss the appropriate management
A COMBINATION OF PIGMENTED VILLONODULAR SYNOVITIS AND SYNOVIAL CHONDROMATOSIS PRESENTING AS A LARGE LOOSE BODY IN KNEE
Authors: Shetty Siddharth , Shetty Vikram , Shetty B. J.P. , Bhat Shubha P. 
Published: May 2020

AbstractIntra-articular calcific lesions of knee usually manifest as loose bodies with symptoms of recurring episodes of locking and restriction of movement. These loose bodies are a consequence of trauma or degeneration of structures in the joint and occasionally may be of inflammatory or neoplastic condition of the synovium.Here we present our case as a rare form of presentation where in our patient had a large loose body in the knee with chronic pain and swelling and restriction of the movements of knee. Arthrotomy, loose body excision and total synovectomy of the knee confirmed coexistence of a dual lesion of synovial chondromatosis of the Hoffa's fat pad with diffuse pigmented villonodular synovitis of the knee joint. The patient made an uneventful recovery and full range of knee movement was restored by end 3 months. These benign conditions have inherent risk of recurrence, and in our case, till the last follow up at 14 months after surgery, he has remained asymptomatic with no clinico radiological evidence of any recurrence.
A Chestnut–A Difficult Challenge for an Endoscopist
Authors: Zykutė Dalia , Zykus Romanas 
Published: May 2020

AbstractTrue foreign bodies are a frequent problem in mentally disabled adults and since ingestion is usually intentional, retrieval can be difficult due to unusual, previously in practice not encountered objects. This case illustrates the laborious effort of removing an esophageal foreign body (FB), a chestnut, in a mentally disabled adult.
COMPARATIVE EVALUATION OF DIABETOGENIC AND MUTAGENIC POTENTIAL OF ARTIFICIAL SWEETENERS - ASPARTAME, ACESULFAME-K AND SUCRALOSE
Authors: S. Shastry C. , K. Yatheesh C. , J. Aswathanarayana B. 
Published: May 2020

Abstract
Objectives: Artificial sweeteners provide the sweetness of sugar without calories. Since from discovery, safety of artificial sweeteners has been controversial as they directly or indirectly link to induce carcinogenic and genotoxic risks. Hence the present study was undertaken to compare the diabetogenic and mutagenic potential of most widely using artificial sweeteners; aspartame, acesulfame-K, and sucralose.
Methods: Diabetic potential is assessed by ascending repeated dose study in which acceptable daily intake (ADI) dose of artificial sweeteners after converting human dose to animal dose using a standard reference table and administered up to 13 weeks with 3 different phases in an ascending manner on experimental rats. Mutagenic potential was accessed by Ames test with and without metabolic activation using Salmonella typhimurium strains TA 97 and TA1535.
Results: At ADI doses between 0-3 weeks, no significant changes but after 13 weeks significant increase was observed in the levels of fasting blood glucose, glycated haemoglobin, total cholesterol, triglyceride, LDL and VLDL in all artificial sweetener groups. Sucralose showed comparatively less increase which was supported by histology reports. In Ames mutagenic assay aspartame, acesulfame-K and sucralose gave negative results.
Conclusion: Aspartame, acesulfame-K and sucralose were found to exhibit diabetogenic effect at higher dose levels but they were safer to use at ADI doses and non mutagenic compounds. Comparatively sucralose is safer than aspartame and acesulfame-K. Hence these artificial sweeteners should be used with caution and over usage is not appreciated.
Single-Balloon Enteroscopy—Guided ERCP in Surgically Altered Anatomy Is Safe and Highly Effective: Results from a Prospective Study
Authors: Pal Partha , Kulkarni Sujay Ashok , Chaudhari Hrushikesh , Ramchandani Mohan , Reddy P. Manohar , Shrimal Pankaj Kumar , Reddy D. Nageshwar , Tandan Manu 
Published: May 2020

Abstract
Objectives Single-balloon enteroscopy (SBE)—assisted endoscopic retrograde cholangiopancreatography (ERCP) has been shown to be cost effective and less technically demanding than other modes of device-assisted enteroscopy. We aimed to evaluate the safety and efficacy of SBE-ERCP in surgically altered anatomy.
Methods This was a prospective cohort study in a high-volume tertiary care gastroenterology center. Patients with surgically altered anatomy requiring pancreaticobiliary interventions, who failed ERCP with a colonoscope/duodenoscope, underwent SBE-ERCP. Diagnostic and therapeutic success, procedure time, and adverse events were evaluated prospectively with short-term follow-up.
Results Twenty-eight patients with surgically altered anatomy (19 males, aged 15–66 years) underwent total 40 SBE-ERCP (23 Roux-en-Y, 17 Billroth II anatomy) procedures. The ERCP diagnosis were hepaticojejunostomy (HJ) stricture (N = 10), HJ stricture and stone (N = 7), common bile duct (CBD) stricture (N = 6), CBD stone (N = 1), pancreaticojejunostomy (PJ) stricture (N = 1), and pancreatic duct (PD) stricture (N = 1). Diagnostic success was achieved in 91.3% (21/23) patients with Roux-en-Y anatomy and 100% (17/17) with Billroth II anatomy. Therapeutic success was achieved in 86.95% (20/23) and 94.1% (16/17) patients with Roux-en-Y and Billroth II anatomy, respectively. In patients with intact papilla (N = 8), diagnostic and therapeutic success were achieved in 100% (8/8) and 75% (6/8) cases, respectively. The mean procedure time for Roux-en-Y and Billroth II anatomy were 64.95 minutes (range 30–110 min) and 38.31 minutes (range 25–60 min), respectively. Immediate complications occurred in 7.5% (3/40) procedures (2 requiring laparotomy, 1 treated endoscopically) in the form of perforation. Among delayed complications, 5 patients had cholangitis and 1 had recurrent cholestasis, which were successfully treated with stent exchange. No other complications occurred over median follow-up of 110 days (30–390 d).
Conclusion SBE-ERCP in surgically altered anatomy requiring pancreatic–biliary intervention appears safe and highly effective with current long ERCP accessories. Further improvement in SBE-ERCP technique and accessories has the potential to make it a promising avenue in surgically altered anatomy.
Pulsed nanosecond air discharge in contact with water: influence of voltage polarity, amplitude, pulse width, and gap distance
Authors: Hamdan Ahmad , Ridani Daniel A , Diamond James , Daghrir Rimeh 
Published: May 2020

Abstract
Plasma technology is a highly promising and advantageous technology for liquid processing. In air in-contact with water, plasma produces highly reactive species (ions, electrons, radicals, photons, etc) that diffuse into the water volume and initiate physical and chemical phenomena of interest, e.g. organic and inorganic pollutant degradation. In this study, we investigate the influence of basic parameters, such as voltage polarity, voltage amplitude, plasma lifetime, and air-gap distance, on the properties of a discharge in air in-contact with deionized water. Specifically, we analyze the electrical characteristics, the plasma behaviour at the water surface, water acidity and conductivity, and the decoloration rate of a standard organic dye (methylene blue). The concentration of the main reactive oxygen and nitrogen species produced in water is also reported. Compared to positive polarity conditions, the negative polarity voltages enhance the decolaration rate of methylene blue. For instance, under negative polarity voltages and while applying 4 and 6 kV, the decoloration rate is relatively low (<30%) and reaches 100% after 25 min of processing at −10 kV. The decoloration rate of MB is also strongly influenced by air-gap distance. Under positive polarity conditions, the decoloration rate decreases from ∼80 to ∼0% as the air-gap distance increases from 0.5 to 7.5 mm, whereas, under negative polarity conditions, the decoloration rate is ∼100%, irrespective of the air-gap distance (0.5 and 4.5 mm).
A dual-domain deep learning-based reconstruction method for fully 3D sparse data helical CT
Authors: Zheng Ao , Gao Hewei , Zhang Li , Xing Yuxiang 
Published: May 2020

Abstract
Helical CT has been widely used in clinical diagnosis. In this work, we focus on a new prototype of helical CT, equipped with sparsely spaced multidetector and multi-slit collimator (MSC) in the axis direction. This type of system can not only lower radiation dose, and suppress scattering by MSC, but also cuts down the manufacturing cost of the detector. The major problem to overcome with such a system, however, is that of insufficient data for reconstruction. Hence, we propose a deep learning-based function optimization method for this ill-posed inverse problem. By incorporating a Radon inverse operator, and disentangling each slice, we significantly simplify the complexity of our network for 3D reconstruction. The network is composed of three subnetworks. Firstly, a convolutional neural network (CNN) in the projection domain is constructed to estimate missing projection data, and to convert helical projection data to 2D fan-beam projection data. This is follwed by the deployment of an analytical linear operator to transfer the data from the projection domain to the image domain. Finally, an additional CNN in the image domain is added for further image refinement. These three steps work collectively, and can be trained end to end. The overall network is trained on a simulated CT dataset based on eight patients from the American Association of Physicists in Medicine (AAPM) Low Dose CT Grand Challenge. We evaluate the trained network on both simulated datasets and clinical datasets. Extensive experimental studies have yielded very encouraging results, based on both visual examination and quantitative evaluation. These results demonstrate the effectiveness of our method and its potential for clinical usage. The proposed method provides us with a new solution for a fully 3D ill-posed problem.
Kappaphobia is the elephant in the fret room
Authors: VanDerMeer B Wieb 
Published: May 2020

Abstract
FRET is both a phenomenon and a spectroscopic technique, capable of measuring one geometric quantity: kappa-squared divided by the sixth power of the donor-acceptor distance. Kappa-squared is often replaced by a constant even though such a replacement may lead to serious errors. Kappaphobia, the fear of kappa or the reluctance to deal with kappa-squared adequately, is a looming presence in the FRET community. Unfortunately, this reluctance, or fear, is often tolerated, and sometimes encouraged. A decrease in kappaphobia will lead to an increase in the impact and success of FRET.
The Brontës in Haute Couture: A Sartorial Adaptation of Literary Texts
Authors: Choe Jian 
Published: May 2020

Abstract
This essay elucidates the ways in which Veronique Branquinho, a contemporary Belgian avant-garde fashion designer, appropriates the literary heritage of the Brontë sisters. A well-read, intellectual visual artist, she has presented collections marked with literary references. Her recent works tap into some key aspects of the Brontë oeuvre, re-envisioning them into highly original sartorial forms. The influence of the Brontës manifests itself in the ethos of Branquinho’s craftsmanship as well as in the individual artefacts that she has created. The designer’s Brontë-inspired dresses with their formal beauty and distinctive aesthetics could be viewed as an intermedial ode to the British writers, her enduring muses, attesting to the universal appeal of the sisters across disciplines.