bluuebunny/crossref_metadata_2025_split huggingface dataset:
Migration of immortalized nasopharyngeal epithelia and carcinoma cells through porous membrane in 3D platforms
Authors: Liu Ziyu , Zhang Weiguan , Pang Stella W. 
Published: May 2020

AbstractIn the present study, 3D biomimetic platforms were fabricated with guiding grating to mimic extracellular matrix topography, porous membrane to resemble the epithelial porous interface and trenches below to represent blood vessels as an in vitro tissue microenvironment. Fabrication technologies were developed to integrate the transparent biocompatible polydimethylsiloxane platforms with preciously controlled dimensions. Cell migration behaviors of an immortalized nasopharyngeal epithelial cell line (NP460) and a nasopharyngeal carcinoma cell line (NPC43) were studied on the 2D and 3D platforms. The NP460 and NPC43 cells traversing through the porous membrane and migrating in the trenches below were studied by time-lapse imaging. Before traversing through the pores, NP460 and NPC43 cells migrated around the pores but NPC43 cells had a lower migration speed with less lamellipodia spreading. After traversing to trenches below, NPC43 cells moved faster with an alternated elongated morphology (mesenchymal migration mode) and round morphology (amoeboid migration mode) compared with only mesenchymal migration mode for NP460 cells. The cell traversing probability through porous membrane on platforms with 30 μm wide trenches below was found to be the highest when the guiding grating was perpendicular to the trenches below and the lowest when the guiding grating was parallel to the trenches below. The present study shows important information on cell migration in complex 3D microenvironment with various dimensions and could provide insight for pathology and treatment of nasopharyngeal carcinoma.
Long non-coding RNA PVT1-mediated miR-543/SERPINI1 axis plays a key role in the regulatory mechanism of ovarian cancer
Authors: Qu Chong , Dai Chunmei , Guo Yahua , Qin Rui , Liu Junbao 
Published: May 2020

Abstract
Purpose: To investigate the regulation mechanism of long non-coding RNA (lncRNA) plasmocytoma variant translocation 1 (PVT1) in ovarian cancer (OC).
Methods: The levels of PVT1, microRNA (miR)-543, serpin peptidase inhibitor-clade I (neuroserpin)-member 1 (SERPINI1) in OC tissues and OVCAR-3, A2780, TOV-112D of OC cell lines were detected by quantitative real-time PCR (qRT-PCR) and Western Blot (WB). Cell proliferation, migration, invasion, apoptosis and the regulatory relationship between genes and target genes were analyzed by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), Transwell, flow cytometry and dual luciferase reporter (DLR). The OC patients were followed up for 5 years to analyze the relationship between PVT1 and 5-year overall survival (OS).
Results: In contrast with miR-543, PVT1 and SERPINI1 were highly expressed in OC tissues and cell lines, and high levels of PVT1 were significantly associated with lower 5-year OS of patients. Down-regulating PVT1 not only inhibited the malignant proliferation, migration and invasion of OC cells, but promoted cell apoptosis. PVT1 regulated miR-543 in a targeted manner, and its overexpression could attenuate the anticancer effect of miR-543 on OC cells. In addition, miR-543 also directly targeted SERPINI1, and miR-543 knockdown weakened the inhibitory effect of down-regulated SERPINI1 on OC progression. Furthermore, we found that PVT1 acted as a competitive endogenous RNA to sponge miR-543, thereby regulating the expression of SERPINI1.
Conclusion: PVT1 can mediate the molecular mechanism of OC by miR-543/SERPINI1 axis regulatory network, which is a new therapeutic direction for OC.
Glecaprevir and Maraviroc are high-affinity inhibitors of SARS-CoV-2 main protease: possible implication in COVID-19 therapy
Authors: Shamsi Anas , Mohammad Taj , Anwar Saleha , AlAjmi Mohamed F. , Hussain Afzal , Rehman Md. Tabish , Islam Asimul , Hassan Md. Imtaiyaz 
Published: May 2020

AbstractDue to the lack of efficient therapeutic options and clinical trial limitations, the FDA-approved drugs can be a good choice to handle Coronavirus disease (COVID-19). Many reports have enough evidence for the use of FDA-approved drugs which have inhibitory potential against target proteins of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). Here, we utilized a structure-based drug design approach to find possible drug candidates from the existing pool of FDA-approved drugs and checked their effectiveness against the SARS-CoV-2. We performed virtual screening of the FDA-approved drugs against the main protease (Mpro) of SARS-CoV-2, an essential enzyme, and a potential drug target. Using well-defined computational methods, we identified Glecaprevir and Maraviroc (MVC) as the best inhibitors of SARS-CoV-2 Mpro. Both drugs bind to the substrate-binding pocket of SARS-CoV-2 Mpro and form a significant number of non-covalent interactions. Glecaprevir and MVC bind to the conserved residues of substrate-binding pocket of SARS-CoV-2 Mpro. This work provides sufficient evidence for the use of Glecaprevir and MVC for the therapeutic management of COVID-19 after experimental validation and clinical manifestations.
Synthesis and evaluation of microwave absorption properties of Fe3O4/Halloysite/polypyrrole nanocomposites
Authors: Maleki Sajjad Tabar , Babamoradi Mohsen , Hajizadeh Zoleikha , Maleki Ali , Rouhi Mojtaba 
Published: May 2020

The Fe3O4 nanoparticles and Fe3O4/Halloysite nanotubes (HNTs) were synthesised by the co‐precipitation method and Fe3O4/HNTs/polypyrrole (PPy) nanocomposites were synthesised by in situ polymerisation of pyrrole monomers on the surface of the Fe3O4/HNTs. The scanning electron microscopy images showed the uniform structure of nanocomposites. The Fourier transform infrared spectra revealed the characteristic peaks of Halloysite and PPy. The Fe3O4/HNTs/PPy nanocomposite exhibits a saturated magnetisation of 6.7 emu/g. The microwave absorption properties of Fe3O4/HNTs/PPy nanocomposites were investigated in the 8–12 GHz (X‐band) range. The value of the minimum reflection loss was obtained − 26.2 dB at 11 GHz for the 3 mm thickness nanocomposite. The results showed the absorbing bandwidth below − 10 dB was broadened to 2.3 GHz.
Use of Organs for Heart Transplantation after Rescue Allocation: Comparison of Outcome with Regular Allocated High Urgent Recipients
Authors: Immohr Moritz , Boeken Udo , Mehdiani Arash , Boettger Charlotte , Aubin Hug , Dalyanoglu Hannan , Erbel Sophia , Scheiber Daniel , Westenfeld Ralf , Akhyari Payam , Lichtenberg Artur 
Published: May 2020

Abstract
Background The number of patients waiting for heart transplantation (HTx) is exceeding the number of actual transplants. Subsequently, waiting times are increasing. One possible solution may be an increased acceptance of organs after rescue allocation. These organs had been rejected by at least three consecutive transplant centers due to medical reasons.
Methods Between October 2010 and July 2019, a total of 139 patients underwent HTx in our department. Seventy (50.4%) of the 139 patients were transplanted with high urgency (HU) status and regular allocation (HU group); the remaining received organs without HU listing after rescue allocation (elective group, n = 69).
Results Donor parameters were comparable between the groups. Thirty-day mortality was comparable between HU patients (11.4%) and rescue allocation (12.1%). Primary graft dysfunction with extracorporeal life support occurred in 26.9% of the elective group with rescue allocated organs, which was not inferior to the regular allocated organs (HU group: 35.7%). No significant differences were observed regarding the incidence of common perioperative complications as well as morbidity and mortality during 1-year follow-up.
Conclusions Our data support the use of hearts after rescue allocation for elective transplantation of patients without HU status. We could show that patients with rescue allocated organs showed no significant disadvantages in the early perioperative morbidity and mortality as well at 1-year follow-up.
Outcomes after Contralateral Anatomic Surgical Resection in Multiple Lung Cancer
Authors: Fourdrain Alex , Bagan Patrick , Georges Olivier , Lafitte Sophie , De Dominicis Florence , Meynier Jonathan , Berna Pascal 
Published: May 2020

Abstract
Background Patients treated surgically for lung cancer may present synchronous or metachronous lung cancers. The aim of this study was to evaluate outcomes after a second contralateral anatomic surgical resection for lung cancer.
Methods We performed a retrospective two-center study, based on a prospective indexed database. Included patients were treated surgically by bilateral anatomic surgical resection for a second primary lung cancer. We excluded nonanatomic resections, benign lesions, and ipsilateral second surgical resections.
Results Between January 2011 and September 2018, 55 patients underwent contralateral anatomic surgical resections for lung cancer, mostly for metachronous cancers. The first surgical resection was a lobectomy in most cases (45 lobectomies: 81.8%, 9 segmentectomies: 16.4%, and 1 bilobectomy: 1.8%), and a video-assisted thoracic surgery (VATS) procedure was used in 23 cases (41.8%). The mean interval between the operations was 38 months, and lobectomy was less frequent for the second surgical resection (35 lobectomies: 63.6% and 20 segmentectomies: 36.4%), with VATS procedures performed in 41 cases (74.5%). Ninety-day mortality was 10.9% (n = 6), and 3-year survival was 77%. Risk factor analysis identified the number of resected segments during the second intervention or the total number of resected segments, extent of resection (lobectomy vs. segmentectomy), surgical approach (thoracotomy vs. VATS), tumor stage, and nodal involvement as potential prognostic factors for long-term survival.
Conclusion A second contralateral anatomic surgical resection for multiple primary lung cancer is possible, with a higher early mortality rate, but acceptable long-term survival, and should be indicated for carefully selected patients.
Feasibility and 3D Planning of a Novel Patient-Specific Instrumentation Technique in Medial Opening-Wedge High Tibial Osteotomy
Authors: Van Genechten Wouter , Van Tilborg Wouter , Van den Bempt Maxim , Van Haver Annemieke , Verdonk Peter 
Published: May 2020

AbstractA novel approach for opening-wedge high tibial osteotomy (OWHTO) with patient-specific instrumentation (PSI) was evaluated for its safety, feasibility, and accuracy. Next, the mechanical medial proximal tibial angle (mMPTA) was assessed as a potential planning angle by investigating the relation with the mechanical femorotibial angle (mFTA). Ten OWHTO cases were 3D planned using the mMPTA and operated with a customized 3D-printed wedge and cast which resembled the intended osteotomy opening. Patients were closely monitored for intraoperative and postoperative complications up to 1 year after surgery. Radiological assessment was conducted on full leg standing radiographs and supine lower limb computed tomography-scans preoperatively and 3 months after surgery. No intraoperative complications or logistical issues during PSI processing were observed. Absolute accuracy outcomes showed a correction error of 1.3° ± 1.1 mMPTA and 0.9° ± 0.6 mFTA with all osteotomies falling in (−2°; + 2°) mFTA around the target. The mMPTA and mFTA were found to have a strong correlation in both 3D (r = 0.842, p = 0.002) and 2D (r = 0.766, p = 0.01) imaging for effective correction. The study confirmed the development of a safe and feasible PSI technique in OWHTO with excellent accuracy outcomes. The strong correlation between the mMPTA and mFTA indicated that soft tissue changes after OWHTO are of minor significance to the final alignment in ligament-stable patients. Finally, the mMPTA was found to be a reliable planning angle in 3D software for obtaining the intended lower limb realignment and its use can therefore be recommended in modern OWHTO planning.
The Rise of Obesity among Total Knee Arthroplasty Patients
Authors: Mohamed Nequesha S. , Wilkie Wayne A. , Remily Ethan A. , Dávila Castrodad Iciar M. , Jean-Pierre Mirlande , Jean-Pierre Nancy , Gbadamosi Wahab A. , Halik Abraham K. , Delanois Ronald E. 
Published: May 2020

AbstractIn the United States, one-third of adults are considered obese, and demand for total knee arthroplasty (TKA) is expected to rise in these patients. Surgeons are reluctant to operate on obese patients, but it is important to understand how obesity has affected TKA utilization. This study utilizes a national database to evaluate incidence, demographics, outcomes, charges, and cost in nonobese, overweight, nonmorbidly obese, and morbidly obese TKA patients. We queried the National Inpatient Sample from 2009 to 2016 for primary TKA patients identifying 4,053,037 nonobese patients, 40,077 overweight patients, 809,649 nonmorbidly obese patients, and 428,647 morbidly obese patients. Chi-square was used to analyze categorical variables, and one-way analysis of variance was used to analyze continuous variables. Nonmorbidly obese and morbidly obese patients represented 23.2% of all TKAs. TKA utilization increased 4.1% for nonobese patients, 121.6% for overweight patients, 73.6% for nonmorbidly obese patients, and 83.9% for morbidly obese patients. Morbidly obese patients were younger (p < 0.001), female (p < 0.001), Black (p < 0.001), poor (p < 0.001), and utilized private insurance (p < 0.001). They also had the longest length of stay (p < 0.001) and the highest mortality rate (p < 0.001). More morbidly obese patients were discharged to other facilities (p < 0.001), and they had the highest rate of complications (p < 0.001). Patients with morbid obesity had the highest charges (p < 0.001), but overweight patients had the highest costs (p < 0.001). The results of this study demonstrate the rise in obese and morbidly obese patients seeking TKAs, which may be reflection of the obesity epidemic in America. Although TKA utilization has increased for morbidly obese patients, this body mass index (BMI) category also has the highest rates of charges and complications, suggesting morbid obesity to be a modifiable risk factor leading to worse surgical and economic outcomes. Obese patients undergoing TKA may benefit from preoperative optimization of their weight, in an effort to reduce the risk of adverse outcomes.
The Ameliorative Effects of Ascorbic Acid on Critical Illness Polyneuropathy in Rodent Sepsis Model
Authors: Sunnetci Silistre Eda , Erbas Oytun 
Published: May 2020

AbstractAlthough the exact pathophysiology of critical illness polyneuropathy (CIP) is still unknown, there are several hypotheses, some of which are increased inflammation and oxidative stress. We used rodent sepsis model in which we induced sepsis through cecal ligation followed by cecal puncture. We then administered ascorbic acid (AA) and evaluated outcomes. The levels of malondialdehyde (MDA), tumor necrosis factor α (TNF-α), interleukins (IL)-6 in the plasma, and heat shock protein-70 (HSP-70) levels in the sciatic nerve were measured, and also electromyography analyses were performed. While plasma MDA, TNF-α, and IL-6 levels were decreased significantly with AA treatment, sciatic nerve levels of HSP-70 were significantly elevated in the AA group. A significant increase in compound muscle action potential (CMAP) amplitude and a significant decrease in CMAP latency were detected in the AA group. We observed healing effects of AA on a rat model of CIP and these effects seem to be related to its anti-inflammatory and antioxidant properties.
Effect of the COVID-19 Pandemic on the Activity of Physicians Working in the Areas of Head and Neck Surgery and Otorhinolaryngology
Authors: Kowalski Luiz Paulo , Imamura Rui , Castro Junior Gilberto de , Marta Gustavo Nader , Chaves Aline Lauda Freitas , Matos Leandro Luongo , Bento Ricardo Ferreira 
Published: May 2020

Abstract
Introduction Coronavirus disease 2019 (COVID-19) is an acute infection caused by the new coronavirus (SARS-CoV-2) and it is highly transmissible, especially through respiratory droplets. To prepare the health system for the care of these patients also led to a restriction in the activity of several medical specialties. Physicians who work with patients affected by diseases of the head and neck region constitute one of the populations most vulnerable to COVID-19 and also most affected by the interruption of their professional activities.
Objective The aim of the present study was to assess the impact of the COVID-19 pandemic on the practice of head and neck surgeons and otorhinolaryngologists in Brazil.
Methods An anonymous online survey of voluntary participation was applied, containing 30 questions regarding demographic aspects, availability of personal protective equipment (PPE), and impact on the routine of head and neck surgeons and otorhinolaryngologists, as well as clinical oncologists and radiation oncologists who work with head and neck diseases.
Results Seven hundred and twenty-nine answers were received in a period of 4 days, ∼ 40 days after the 1st confirmed case in Brazil. With professionals working in public and private services, there was a high level of concerns with the disease and its consequences, limited availability of PPE and a significant decrease in the volume of specialized medical care.
Conclusion The study demonstrated a direct impact of the COVID-19 pandemic on the clinical practice of specialties related to the treatment of patients with diseases of the head and neck region already in the beginning of the illness management in Brazil.
Rise in Cases of Alcohol Withdrawal following Lockdown in Dry District of Wardha
Authors: Behere Prakash B. , Behere Aniruddh P. , Chowdhury Debolina 
Published: May 2020

Abstract
On March 11, 2020, the World Health Organization declared the SARS-CoV-2 outbreak to be a pandemic which has sent all countries in a frenzy. We cannot be callous about treatment of non-COVID-19-related patients. The Wardha district of Maharashtra is a declared “dry area” which makes the sale, purchase, and consumption of alcohol illegal. On March 24, 2020, Indians were informed of a 21-day long lockdown which was subsequently extended for another 3 weeks during which all modes of public transportation, educational institutions, offices, and other nonessential businesses were closed and people expected to remain at home unless necessary. Since then admissions of alcohol-dependent patients showed a spike from14% in the prelockdown period to 27% of patients of alcohol dependence syndrome. It is most likely due to poor availability of liquor, inability to earn money to buy the alcohol, or restricted movement of individuals.
The 6-4 photoproduct is the trigger of UV-induced replication blockage and ATR activation
Authors: Hung Kai-Feng , Sidorova Julia M. , Nghiem Paul , Kawasumi Masaoki 
Published: May 2020

Significance
Solar UV generates abundant carcinogenic DNA lesions, and consequently, cells need to cope with these deleterious lesions to survive UV damage. This study used lesion-specific photolyases to isolate the biologically relevant effects of each major type of UV-induced DNA lesions (cyclobutane pyrimidine dimers [CPDs] and 6-4 photoproducts [6-4PPs]) on ATR activation, a crucial DNA damage response. A newly developed, flow cytometric single-cell analysis of UV-induced DNA lesions, thymidine analog incorporation, and DNA damage response enabled unprecedented determination of cellular events following UV irradiation. The striking finding is that only 6-4PPs, the shorter-lived and less abundant lesion type, cause replication blockage and activation of the ATR DNA damage response.
The Paks Pact
Authors: Egres Dorottya , Petschner Anna 
Published: May 2020

Abstract
Hungary’s only nuclear power plant in Paks can operate until the 2030s which prompted a discussion about an expansion. The first section of this paper identifies the eight most common topoi of the expansion: economy, finance, energy supply, safety, environment, legality, ethics and relationship with Russia. Topoi are considered subject-matters of argumentation with which participants of a controversy convey their standpoints. In the second section, topos analysis is used to explore the argumentation of two political bodies, an environmental protectionist organization and two scientific institutions. Results show that the argumentation style of the examined actors differ in the openness of their standpoints, in their calls to action and the frequency of the employed topoi.
Particularist understanding of CSR marketing visual arguments
Authors: Csordás Hédi , Ziegler Zsolt 
Published: May 2020

Abstract
We argue that understanding visual arguments is underdetermined. We address the question concerning how controversial understandings of visual arguments can be handled only by particularism. The distinction between the generalist and the particularist stance taken from moral philosophy offers a way to answer this question. On the particularist view visual principles are incapable of that feat because the visual meaning of a particular visual stimulus is always context-dependent. Instead of appealing to visual principles, the particularist would contrast different situations in a way that their relevant features resemble each other. Understanding a visual argument and the involving context requires analogical reasoning that can be accounted only by particularism. Visual arguments’ valid interpretation can be grasped only within a particularist-like framework.
ELEna
Authors: Jiménez Palmero Diego , Pro José Luis , Salguero-Lamillar Francisco José , Quesada José Francisco 
Published: May 2020

Abstract
This paper focuses on the description of the controversies that arise from the interdisciplinary work necessary for the creation of an on-line platform for learning Spanish as a foreign language, which we call ELEna. The platform develops the learning of written expression skills, thus promoting written production and interaction, the most demanded areas for the students of foreign languages nowadays. In this way, it has been necessary to create an interdisciplinary team made up of linguists and computer scientists who are specialized in the fields of Spanish language teaching and in the creation of interactive on-line platforms. This fact has led to a series of challenges that we present below with their respective solutions in pursuit of scientific progress.
What is the meaning of biodiversity?
Authors: Barrotta Pierluigi , Gronda Roberto 
Published: May 2020

Abstract
The preservation of biodiversity is one of the greatest concerns of our age. However, a satisfactory operational definition of biodiversity is still lacking, and it is likely that it won’t be achieved in the near future. In their practice of measurement scientists adopt a pluralistic and multi-dimensional metric – an approach which is widely accepted, but not theoretically justified. The goal of this paper is to account for such pluralistic approach. Our solution is pragmatist: we hold that the pragmatic maxim highlights the fact-value entanglement intrinsic to the concept of biodiversity. On this basis, we argue that the axiological dimension is essential to the meaning of the concept since its extension cannot be fixed independently of it.
Science and democracy
Authors: Pombo Olga 
Published: May 2020

Abstract
The article analyses the relationship between science and democracy on basis of a very specific point of view. Three arguments are put forward to defend that. In its historical origin, in its linguistic roots and in its institutional basis, science is (and continues to be) a democratic endeavor. The article very briefly discusses the decisive changings in the relation between science and political, economic and military power that took place during the XX century and stresses the cognitive relevance of a set of universal institutions which, all along the history of science, provide the conditions of possibility for science to exist and develop. Finally, even if today those universal institutions face big transformations, the possibility for science to remain a free democratic endeavor is questioned and argued.