bluuebunny/crossref_metadata_2025_split huggingface dataset:
The importance of precision: differences in characteristics associated with levels of food security among college students
Authors: Soldavini Jessica , Berner Maureen 
Published: March 2020

AbstractObjective:To estimate the prevalence of high, marginal, low and very low food security among a sample of college students and identify characteristics associated with the four different food security status levels and note differences in associations from when food security status is classified as food-secure v. food-insecure.Design:Cross-sectional online survey.Setting:A large public university in North Carolina.Participants:4829 college students who completed an online survey in October and November 2016.Results:Among study participants, 56·2 % experienced high, 21·6 % experienced marginal, 18·8 % experienced low and 3·4 % experienced very low food security. Characteristics significantly associated with food security status when using the four-level variable but not two-level variable were age, international student status and weight status. Characteristics that significantly differed between the marginal and high food security groups included age, race/ethnicity, year in school, international student status, employment status, financial aid receipt, perceived health rating, cooking frequency and participation in an on-campus meal plan. Characteristics with differences in significant associations between the low and very low food security groups were gender, international student status, having a car, weight status and participation in an on-campus meal plan. Even where similarities in the direction of association were seen, there were often differences in magnitude.Conclusions:We found differences in characteristics associated with food security status when using the four-level v. two-level food security status variable. Future studies should look separately at the four levels, or at least consider separating the marginal and high food-secure groups.
Investigation of Midlatitude Nighttime Ionospheric E‐F Coupling and Interhemispheric Coupling by Using COSMIC GPS Radio Occultation Measurements
Authors: Liu Yi , Zhou Chen , Xu Tong , Wang Zhuangkai , Tang Qiong , Deng Zhongxin , Chen Guanyi 
Published: March 2020

AbstractThis study presents the statistical features of ionospheric E‐F coupling and interhemispheric coupling at midlatitudes. Nighttime ionospheric E and F region irregularity occurrences are investigated by using Constellation Observing System for Meteorology, Ionosphere, and Climate (COSMIC) radio occultation measurements during 2006–2018. We presented four typical events of simultaneous observations of E and F region irregularities in the local hemisphere and F region irregularities in both conjugate hemispheres. Results of case studies indicate the coincidence of ionospheric irregularities in both E and F regions, as well as the coincidence of ionospheric irregularities in both F regions of two conjugate hemispheres. Statistical analysis of occurrences of E and F region irregularities shows that the concurrence of ionospheric irregularities in both E and F regions reaches a maximum (nearly 70%) during June (December) solstice in the Northern (Southern) Hemisphere. Nearly 50% (45%) of the F region irregularity in the Northern (Southern) Hemisphere occurs simultaneously with F region irregularity occurrence in the Southern (Northern) Hemisphere during December (June) solstice. These results provide the observational evidences showing that the effect of E‐F coupling and interhemispheric coupling may be important in generation of midlatitude nighttime F region irregularity. In addition, our results also reveal that mapping efficiency of electrodynamic coupling has a different seasonal variation in the Northern and Southern Hemispheres, which shows dependence on both the integrated Pedersen conductivity of F region (
) and the integrated Hall conductivity of ES layer (
).
3D microgroove electrical impedance sensing to examine 3D cell cultures for antineoplastic drug assessment
Authors: Pan Yuxiang , Jiang Deming , Gu Chenlei , Qiu Yong , Wan Hao , Wang Ping 
Published: March 2020

AbstractIn recent decades, three-dimensional (3D) cancer cell models have attracted increasing interest in the field of drug screening due to their significant advantages in more accurate simulations of heterogeneous tumor behavior in vivo compared to two-dimensional models. Furthermore, drug sensitivity testing based on 3D cancer cell models can provide more reliable in vivo efficacy prediction. The gold standard fluorescence staining is hard to achieve real-time and label-free viability monitoring in 3D cancer cell models. In this study, a microgroove impedance sensor (MGIS) was specially developed for the dynamic and noninvasive monitoring of 3D cell viability. 3D cancer cells were trapped in microgrooves with gold electrodes on opposite walls for in situ impedance measurement. The change in the number of live cells caused inversely proportional changes to the impedance magnitude of the entire cell/Matrigel construct and reflected the proliferation and apoptosis of the 3D cells. It was confirmed that the 3D cell viability detected by the MGIS was highly consistent with the standard live/dead staining by confocal microscope characterization. Furthermore, the accuracy of the MGIS was validated quantitatively using a 3D lung cancer model and sophisticated drug sensitivity testing. In addition, the parameters of the MGIS in the measurement experiments were optimized in detail using simulations and experimental validation. The results demonstrated that the MGIS coupled with 3D cell culture would be a promising platform to improve the efficiency and accuracy of cell-based anticancer drug screening in vitro.
The non-receptor tyrosine phosphatase type 14 blocks caveolin-1-enhanced cancer cell metastasis
Authors: Díaz-Valdivia Natalia I. , Díaz Jorge , Contreras Pamela , Campos América , Rojas-Celis Victoria , Burgos-Ravanal Renato A. , Lobos-González Lorena , Torres Vicente A. , Perez Viviana I. , Frei Balz , Leyton Lisette , Quest Andrew F. G. 
Published: March 2020

AbstractCaveolin-1 (CAV1) enhanced migration, invasion, and metastasis of cancer cells is inhibited by co-expression of the glycoprotein E-cadherin. Although the two proteins form a multiprotein complex that includes β-catenin, it remained unclear how this would contribute to blocking the metastasis promoting function of CAV1. Here, we characterized by mass spectrometry the protein composition of CAV1 immunoprecipitates from B16F10 murine melanoma cells expressing or not E-cadherin. The novel protein tyrosine phosphatase PTPN14 was identified by mass spectrometry analysis exclusively in co-immunoprecipitates of CAV1 with E-cadherin. Interestingly, PTPN14 is implicated in controlling metastasis, but only few known PTPN14 substrates exist. We corroborated by western blotting experiments that PTPN14 and CAV1 co-inmunoprecipitated in the presence of E-cadherin in B16F10 melanoma and other cancer cells. Moreover, the CAV1(Y14F) mutant protein was shown to co-immunoprecipitate with PTPN14 even in the absence of E-cadherin, and overexpression of PTPN14 reduced CAV1 phosphorylation on tyrosine-14, as well as suppressed CAV1-enhanced cell migration, invasion and Rac-1 activation in B16F10, metastatic colon [HT29(US)] and breast cancer (MDA-MB-231) cell lines. Finally, PTPN14 overexpression in B16F10 cells reduced the ability of CAV1 to induce metastasis in vivo. In summary, we identify here CAV1 as a novel substrate for PTPN14 and show that overexpression of this phosphatase suffices to reduce CAV1-induced metastasis.
A common polymorphism in the retinoic acid pathway modifies adrenocortical carcinoma age-dependent incidence
Authors: Surakhy Mirvat , Wallace Marsha , Bond Elisabeth , Grochola Lukasz Filip , Perez Husein , Di Giovannantonio Matteo , Zhang Ping , Malkin David , Carter Hannah , Parise Ivy Zortea S. , Zambetti Gerard , Komechen Heloisa , Paraizo Mariana M. , Pagadala Meghana S. , Pinto Emilia M. , Lalli Enzo , Figueiredo Bonald C. , Bond Gareth L. 
Published: March 2020

Abstract
Background
Genome-wide association studies (GWASs) have enriched the fields of genomics and drug development. Adrenocortical carcinoma (ACC) is a rare cancer with a bimodal age distribution and inadequate treatment options. Paediatric ACC is frequently associated with TP53 mutations, with particularly high incidence in Southern Brazil due to the TP53 p.R337H (R337H) germline mutation. The heterogeneous risk among carriers suggests other genetic modifiers could exist.

Methods
We analysed clinical, genotype and gene expression data derived from paediatric ACC, R337H carriers, and adult ACC patients. We restricted our analyses to single nucleotide polymorphisms (SNPs) previously identified in GWASs to associate with disease or human traits.

Results
A SNP, rs971074, in the alcohol dehydrogenase 7 gene significantly and reproducibly associated with allelic differences in ACC age-of-onset in both cohorts. Patients homozygous for the minor allele were diagnosed up to 16 years earlier. This SNP resides in a gene involved in the retinoic acid (RA) pathway and patients with differing levels of RA pathway gene expression in their tumours associate with differential ACC progression.

Conclusions
These results identify a novel genetic component to ACC development that resides in the retinoic acid pathway, thereby informing strategies to develop management, preventive and therapeutic treatments for ACC.
HIF1A activates the transcription of lncRNA RAET1K to modulate hypoxia-induced glycolysis in hepatocellular carcinoma cells via miR-100-5p
Authors: Zhou Yufan , Huang Yun , Hu Kuan , Zhang Zeyu , Yang Jiajin , Wang Zhiming 
Published: March 2020

AbstractHepatocellular carcinoma (HCC) remains the primary cause of cancer-related death. Metabolic change is the major characteristic of cancer. The present study attempted to investigate the regulatory mechanisms of HCC energy metabolism from the perspective of noncoding RNA regulation of HIF1A and LDHA. The expression of miR-100-5p expression was significantly suppressed in HCC tissue samples and HCC cell lines under 1% O2-induced hypoxia. miR-100-5p overexpression significantly suppressed hypoxia-induced increases in lactate concentration and glucose uptake. Exposure to 1% O2 induced HIF1A protein and reduced miR-100-5p expression, while HIF1A silencing dramatically rescued miR-100-5p expression upon 1% O2 exposure. In addition, 1% O2-induced increases in lactate concentration and glucose uptake were also suppressed by HIF1A silencing. Next, by analyzing available data in TCGA, we found that lncRNA RAET1K was correlated with HIF1A and miR-100-5p.LncRNA RAET1K could downregulate the expression of miR-100-5p by acting as a sponge, while HIF1A bound the lncRNA RAET1K promoter region to activate its transcription. LncRNA RAET1K silencing significantly suppressed HCC cell proliferation and invasion and also suppressed hypoxia-induced increases in lactate concentration and glucose uptake, while miR-100-5p inhibition reversed the effects of lncRNA RAET1K silencing on hypoxia-induced glycolysis in HCC cells. Finally, the expression of HIF1A, lncRNA RAET1K, and LDHA was upregulated in HCC tissue specimens; the expression of miR-100-5p was negatively related to HIF1A, lncRNA RAET1K, and LDHA; and HIF1A, lncRNA RAET1K, and LDHA were positively correlated with each other. In conclusion, the HIF1A/lncRNA RAET1K/miR-100-5p axis modulates hypoxia-induced glycolysis in HCC cells and might affect HCC progression.
Effects of substituting eggs for high-carbohydrate breakfast foods on the cardiometabolic risk-factor profile in adults at risk for type 2 diabetes mellitus
Authors: Maki Kevin C. , Palacios Orsolya M. , Kramer Melvyn W. , Trivedi Rupal , Dicklin Mary R. , Wilcox Meredith L. , Maki Cathleen E. 
Published: March 2020

Abstract
Objectives
To assess effects of egg-based versus non-egg, higher-carbohydrate (CHO) breakfast meals on cardiometabolic health markers in overweight or obese adults with prediabetes and/or metabolic syndrome.

Methods
This randomized, crossover study included two 4-week dietary interventions, separated by a ≥4-week washout. Subjects incorporated into their habitual diets breakfast meals containing either 2 eggs/day for 6 days/week (Egg condition), or energy-matched, non-egg, higher-CHO-based foods (Non-Egg condition). Dietary intakes, insulin sensitivity, and other CHO metabolism indices, lipid biomarkers, high-sensitivity C-reactive protein, and blood pressures were measured.

Results
Thirty men and women with mean age 54.1 ± 1.9 years and body mass index 31.9 ± 0.7 kg/m2 provided data. Neither diet condition significantly altered insulin sensitivity indices, but the homeostasis model assessment for insulin resistance was significantly (p = 0.028) higher after the Non-Egg vs. the Egg condition. Low-density lipoprotein cholesterol (LDL-C) was decreased from baseline (119 mg/dL) by 2.9 and 6.0% with Egg and Non-Egg breakfasts, respectively (p = 0.023). Systolic blood pressure was reduced from baseline (127 mm Hg) by 2.7 and 0.0% with Egg and Non-Egg, respectively (p = 0.018). Diet records indicated 149 kcal/day higher (p = 0.008) energy intake from non-study foods during the Egg condition; however, weight change from baseline did not differ between conditions.

Conclusion
Compared with the baseline diet, consumption of 12 eggs/week for 4 weeks at breakfast was associated with less reduction in LDL-C, and more lowering of systolic blood pressure, than observed with non-egg-based, energy-matched, control foods higher in CHO.
Common genetic variation in the Angelman syndrome imprinting centre affects the imprinting of chromosome 15
Authors: Beygo Jasmin , Grosser Christian , Kaya Sabine , Mertel Claudia , Buiting Karin , Horsthemke Bernhard 
Published: March 2020

AbstractAngelman syndrome (AS) is a rare neurogenetic imprinting disorder caused by the loss of function of UBE3A. In ~3–5% of AS patients, the disease is due to an imprinting defect (ID). These patients lack DNA methylation of the maternal SNRPN promotor so that a large SNRPN sense/UBE3A antisense transcript (SNHG14) is expressed, which silences UBE3A. In very rare cases, the ID is caused by a deletion of the AS imprinting centre (AS-IC). To search for sequence alterations, we sequenced this region in 168 patients without an AS-IC deletion, but did not detect any sequence alteration. However, the AS-IC harbours six common variants (five single nucleotide variants and one TATG insertion/deletion variant), which constitute five common haplotypes. To determine if any of these haplotypes is associated with an increased risk for an ID, we investigated 119 informative AS-ID trios with the transmission disequilibrium test, which is a family-based association test that measures the over-transmission of an allele or haplotype from heterozygous parents to affected offspring. By this we observed maternal over-transmission of haplotype H-AS3 (p = 0.0073). Interestingly, H-AS3 is the only haplotype that includes the TATG deletion allele. We conclude that this haplotype and possibly the TATG deletion, which removes a SOX2 binding site, increases the risk for a maternal ID and AS. Our data strengthen the notion that the AS-IC is important for establishing and/or maintaining DNA methylation at the SNRPN promotor and show that common genetic variation can affect genomic imprinting.
A long-term monitoring dataset of fish assemblages in rocky tidepools on the northern coast of Taiwan
Authors: Ho Lin-Tai , Wang Shen-Chih , Shao Kwang-Tsao , Chen I-Shiung , Chen Hungyen 
Published: March 2020

AbstractThe assemblage of fish species in the rocky intertidal zone is highly affected by the destructive impact of human activities and has an extended impact on land-sea interactions. There are a few long-term research projects that have focused on rocky intertidal ecosystems, especially on the resident fish community. Here, we describe a long-term time series dataset of fish collected by counting the number of anesthetized fishes at sampling stations in rocky tidepools in the intertidal zones on the northern coast of Taiwan. The species assemblages were monitored seasonally at three stations from 1999 to 2018. In total, 144 samples containing 1,577 individuals belonging to 106 species were recorded in the surveys. The resulting data can be used as background information for conservation and resilience studies of the fish community in coastal areas and to establish reasonable conservation strategies. This study presents valuable data to ecologists and fisheries biologists interested in understanding the temporal patterns of species abundance, richness, and composition in relation to environmental factors, climate change, and anthropogenic pressures.
Alterations of functional connectivities associated with autism spectrum disorder symptom severity: a multi-site study using multivariate pattern analysis
Authors: Liu Xingdan , Huang Huifang 
Published: March 2020

AbstractAutism spectrum disorder (ASD) is a highly heterogeneous neurodevelopmental disorder. The estimation of ASD severity is very important in clinical practice due to providing a more elaborate diagnosis. Although several studies have revealed some resting-state functional connectivities (RSFCs) that are related to the ASD severity, they have all been based on small-sample data and local RSFCs. The aim of the present study is to adopt multivariate pattern analysis to investigate a subset of connectivities among whole-brain RSFCs that are more contributive to ASD severity estimation based on large-sample data. Regression estimation shows a Pearson correlation value of 0.5 between the estimated and observed severity, with a mean absolute error of 1.41. The results provide obvious evidence that some RSFCs undergo notable alterations with the severity of ASD. More importantly, these selected RSFCs have an abnormality in the connection modes of the inter-network and intra-network connections. In addition, these selected abnormal RSFCs are mainly associated with the sensorimotor network, the default mode network, and inter-hemispheric connectivities, while exhibiting significant left hemisphere lateralization. Overall, this study indicates that some RSFCs suffer from abnormal alterations in patients with ASD, providing additional evidence of large-scale functional network alterations in ASD.
Angiotensin II represses Npr1 expression and receptor function by recruitment of transcription factors CREB and HSF-4a and activation of HDACs
Authors: Arise Kiran K. , Kumar Prerna , Garg Renu , Samivel Ramachandran , Zhao Hanqing , Pandya Krishna , Nguyen Christian , Lindsey Sarah , Pandey Kailash N. 
Published: March 2020

AbstractThe two vasoactive hormones, angiotensin II (ANG II; vasoconstrictive) and atrial natriuretic peptide (ANP; vasodilatory) antagonize the biological actions of each other. ANP acting through natriuretic peptide receptor-A (NPRA) lowers blood pressure and blood volume. We tested hypothesis that ANG II plays critical roles in the transcriptional repression of Npr1 (encoding NPRA) and receptor function. ANG II significantly decreased NPRA mRNA and protein levels and cGMP accumulation in cultured mesangial cells and attenuated ANP-mediated relaxation of aortic rings ex vivo. The transcription factors, cAMP-response element-binding protein (CREB) and heat-shock factor-4a (HSF-4a) facilitated the ANG II-mediated repressive effects on Npr1 transcription. Tyrosine kinase (TK) inhibitor, genistein and phosphatidylinositol 3-kinase (PI-3K) inhibitor, wortmannin reversed the ANG II-dependent repression of Npr1 transcription and receptor function. ANG II enhanced the activities of Class I histone deacetylases (HDACs 1/2), thereby decreased histone acetylation of H3K9/14ac and H4K8ac. The repressive effect of ANG II on Npr1 transcription and receptor signaling seems to be transduced by TK and PI-3K pathways and modulated by CREB, HSF-4a, HDACs, and modified histones. The current findings suggest that ANG II-mediated repressive mechanisms of Npr1 transcription and receptor function may provide new molecular targets for treatment and prevention of hypertension and cardiovascular diseases.
High-Gain Metasurface in Polyimide On-Chip Antenna Based on CRLH-TL for Sub-Terahertz Integrated Circuits
Authors: Alibakhshikenari Mohammad , Virdee Bal S. , See Chan H. , Abd-Alhameed Raed A. , Falcone Francisco , Limiti Ernesto 
Published: March 2020

AbstractThis paper presents a novel on-chip antenna using standard CMOS-technology based on metasurface implemented on two-layers polyimide substrates with a thickness of 500 μm. The aluminium ground-plane with thickness of 3 μm is sandwiched between the two-layers. Concentric dielectric-rings are etched in the ground-plane under the radiation patches implemented on the top-layer. The radiation patches comprise concentric metal-rings that are arranged in a 3 × 3 matrix. The antennas are excited by coupling electromagnetic energy through the gaps of the concentric dielectric-rings in the ground-plane using a microstrip feedline created on the bottom polyimide-layer. The open-ended feedline is split in three-branches that are aligned under the radiation elements to couple the maximum energy. In this structure, the concentric metal-rings essentially act as series left-handed capacitances CL that extend the effective aperture area of the antenna without affecting its dimensions, and the concentric dielectric rings etched in the ground-plane act as shunt left-handed inductors LL, which suppress the surface-waves and reduce the substrates losses that leads to improved bandwidth and radiation properties. The overall structure behaves like a metasurface that is shown to exhibit a very large bandwidth of 0.350–0.385 THz with an average radiation gain and efficiency of 8.15dBi and 65.71%, respectively. It has dimensions of 6 × 6 × 1 mm3 that makes it suitable for on-chip implementation.
Hurricane-Induced Rainfall is a Stronger Predictor of Tropical Forest Damage in Puerto Rico Than Maximum Wind Speeds
Authors: Hall Jazlynn , Muscarella Robert , Quebbeman Andrew , Arellano Gabriel , Thompson Jill , Zimmerman Jess K. , Uriarte María 
Published: March 2020

AbstractProjected increases in cyclonic storm intensity under a warming climate will have profound effects on forests, potentially changing these ecosystems from carbon sinks to sources. Forecasting storm impacts on these ecosystems requires consideration of risk factors associated with storm meteorology, landscape structure, and forest attributes. Here we evaluate risk factors associated with damage severity caused by Hurricanes María and Irma across Puerto Rican forests. Using field and remote sensing data, total forest aboveground biomass (AGB) lost to the storms was estimated at 10.44 (±2.33) Tg, ca. 23% of island-wide pre-hurricane forest AGB. Storm-related rainfall was a stronger predictor of forest damage than maximum wind speeds. Soil water storage capacity was also an important risk factor, corroborating the influence of rainfall on forest damage. Expected increases of 20% in hurricane-associated rainfall in the North Atlantic highlight the need to consider how such shifts, together with high speed winds, will affect terrestrial ecosystems.