bluuebunny/crossref_metadata_2025_split huggingface dataset:
Adjuvant chemotherapy could not bring survival benefit to HR-positive, HER2-negative, pT1b-c/N0-1/M0 invasive lobular carcinoma of the breast: a propensity score matching study based on SEER database
Authors: Hu Guangfu , Hu Guangxia , Zhang Chengjiao , Lin Xiaoyan , Shan Ming , Yu Yanmin , Lu Yongwei , Niu Ruijie , Ye Hui , Wang Cheng , Xu Cheng 
Published: January 2020

Abstract
Background : The benefit of adjuvant chemotherapy in invasive lobular carcinoma (ILC) is still unclear. The objective of the current study was to elucidate the effectiveness of adjuvant chemotherapy in hormone receptor (HR)-positive, human epidermal growth factor receptor 2 (HER2)-negative, pT1b-c/N0-1/M0 ILC.
Methods: Based on Surveillance, Epidemiology, and End-Results (SEER) database, we identified original 12334 HR-positive, HER2-negative, pT1b-c/N0-1/M0 ILC patients, who were then divided into adjuvant chemotherapy group and control group. End-points were overall survival (OS) and breast cancer-specific mortality (BCSM). Aiming to minimize the selection bias of baseline characteristics, Propensity Score Matching (PSM) method was used.
Results : In a total of 12334 patients with HR-positive, HER2-negative, pT1b-c/N0-1/M0 ILC, 1785 patients (14.5%) were allocated into adjuvant chemotherapy group and 10549 (85.5%) into control group. Used PSM, the 1785 patients in adjuvant chemotherapy group matched to the 1785 patients in control group. By Kaplan-Meier survival analyses, we observed no beneficial effect of adjuvant chemotherapy on OS in both original samples ( P =0.639) and matched samples ( P =0.962), however, ineffective or even contrary results of adjuvant chemotherapy on BCSM both in original samples (P =0.001) and in matched samples ( P= 0.002). In both original and matched multivariate Cox models, we observed ineffectiveness of adjuvant chemotherapy on OS (hazard ratio (HR) for overall survival = 0.82, 95% confidence interval (CI) [0.62-1.09]; P =0.172 and HR=0.90, 95%CI [0.65-1.26]; P =0.553, respectively), unexpectedly promoting effect of adjuvant chemotherapy on BCSM (HR=2.33, 95%CI [1.47-3.67]; P =0.001 and HR=2.41, 95%CI [1.32-4.39]; P =0.004, respectively). Standard surgery was beneficial to the survival of patients. Lymph node metastasis was detrimental to survival and radiotherapy brought survival benefit in original samples, but two issues had unobvious effect in matched samples.
Conclusion: In this study, adjuvant chemotherapy did not improve survival for patients with HR-positive, HER2-negative pT1b-c/N0-1/M0 ILC.
Individualized procedures for splenic artery dissection during laparoscopic distal pancreatectomy.
Authors: Wada Yusuke , Aoki Takeshi , Murakami Masahiko , Fujimori Akira , Koizumi Tomotake , Kusano Tomokazu , Matsuda Kazuhiro , Nogaki Koji , Hakozaki Tomoki , Shibata Hideki , Tomioka Kodai 
Published: January 2020

Abstract
[Background] There are no established standard criteria for choosing the most appropriate procedure of splenic artery dissection during laparoscopic distal pancreatectomy (LDP). The aim of this study was to evaluate the clinical benefits of individualized procedures for splenic artery dissection during LDP based on the variations in arterial structure visualized on preoperative three-dimensional computed tomography (3D-CT).
[Methods] Patients who underwent LDP following 3D-CT at a single center were retrospectively evaluated. 3D-CT images were used to construct virtual 3D laparoscopic images for surgical planning. The splenic artery was classified into two major anatomic types: type S that curves and runs suprapancreatic and type D that runs straight and dorsal to the pancreas. Splenic artery dissection was planned according to these two variations, with type S dissected using ansuprapancreatic approach and type D using a dorsal approach.
[Results] Type-specific dissection was applied for 30 patients: 25 (83%) with type S and 5 (17%) with type D splenic artery anatomies. In 25 (83%) patients, the splenic artery was successfully dissected using the planned surgical procedure, whereas the surgical plan had to be altered in 5 cases (17%) due to difficulty in dissecting the splenic artery.
[Conclusion] The individualized procedures for splenic artery dissection according to anatomic variations visualized on 3D-CT images can help improve the success and safety of LDP.
Epigenetic induction of tumor stemness via the Lipopolysaccharide-TET3-HOXB2 signaling axis in esophageal squamous cell carcinoma
Authors: Xu Fengkai , Liu Zhonghe , Liu Ronghua , Lu Chunlai , Wang Lin , Mao Wei , Zhu Qiaoliang , Shou Huankai , Zhang Kunpeng , Li Yin , Chu Yiwei , Gu Jie , Ge Di 
Published: January 2020

Abstract
Background: Esophageal squamous cell cancer (ESCC) is one kind of frequent digestive tumor. The inflammatory environment plays an important role in the tumorigenesis and development of ESCC. Cancer stem cells are a small group of tumor cells with stem cell characteristics, which can potentially hinder the tumor management and treatment.
Methods: ELISA was performed to detect the lipopolysaccharide concentration in cancer tissues. qPCR, Western blot, FACS, Immunohistochemistry, Immunofluorescence and Dot blot were applied to detect target genes expression. CCK-8, Colony-formation, Transwell, Sphere and Xenograft were conducted to investigate the function of cells, influenced by risk factors. The survival curve was drawn with the Kaplan-Meier product limit estimator. Nano-hmC-Seal-seq was utilized to detect the downstream target of TET3. ChIP-qPCR was adopted to demonstrate the transcriptional regulation of stem cell-associated genes by HOXB2.
Results: Lipopolysaccharide concentration was significantly up-regulated in ESCC. High concentration of lipopolysaccharide stimulation induced the stemness of ESCC cells. TET3 expression was elevated with lipopolysaccharide stimulation via p38/ERK-MAPK pathway in ESCC and negatively correlated with patients’ survival. TET3 induced the stemness of ESCC cells. Nano-hmC-Seal-seq showed that TET3 overexpression led to a significant increase in 5hmC levels of HOXB2 gene region, which was thus identified as the downstream target of TET3. The binding of HOXB2 to NANOG and cMYC was veriﬁed by ChIP-qPCR.
Conclusions: Lipopolysaccharide served as a tumor promotor in ESCC by inducing cancer cell stemness through the activation of a LPS-TET3-HOXB2 signaling axis, which might provide a novel therapeutic strategy for ESCC.
Intestinal flora imbalance affects bile acid metabolism and is associated with gallstone formation
Authors: Wang Qiang , Hao Chenjun , Yao Wenchao , Zhu Defu , Lu Haifeng , Li Long , Ma Biao , Xue Dongbo , Zhang Weihui 
Published: January 2020

Abstract
Background: The gut microbiota participates in the metabolism of substances and energy, promotes the development and maturation of the immune system, forms the mucosal barrier, and protects the host from pathogen attacks. Although the pathogenesis of cholesterol gallstones is still not clear, studies have suggested that gut microbiota dysbiosis plays an important role in their formation. Methods: Microbial DNA from faeces of normal control patients and those of patients with calculi was subjected to 16S rRNA gene sequencing to detect gene expression changes in intestinal microbes. ELISA kits were used to measure free bile acids, secondary bile acids and coprostanol according to the manufacturer’s instructions. The relationship between flora and their metabolites was then analysed. Results: In the gallstone group, the diversity of intestinal bacteria and the abundances of certain phylogroups were significantly decreased (p<0.05), especially Firmicutes (p<0.05), the largest phylum represented by the gut microbiota. This study found an increase in free bile acids (p<0.001) and secondary bile acids (p<0.01) in the enterohepatic circulation. Bile salt hydrolase activity was not related to the abundances of BSH-active bacteria. 7a-dehydroxylating gut bacteria were significantly increased (p<0.01), whereas cholesterol-lowering bacteria were significantly reduced (p<0.05). The Ruminococcus gnavus group could be used as a biomarker to distinguish the gallstone group from the control group. Conclusion: We conclude that intestinal flora imbalance affects bile acid and cholesterol metabolism and is associated with gallstone formation.
Proportion of NAFLD patients with normal ALT value in overall NAFLD patients: a systematic review and meta-analysis
Authors: Ma Xuefeng , Liu Shousheng , Zhang Jie , Dong Mengzhen , Wang Yifen , Wang Mengke , Yongning Xin 
Published: January 2020

Abstract
Background: ALT value is often used to reflect the hepatic inflammation and injury in NAFLD patients, but many studies proved that ALT values were normal in many NAFLD patients. The aim of this study was to identify the summarized proportion of NAFLD patients with normal ALT value in the overall NAFLD patients. Methods: Electronic databases PubMed, EMBASE, Ovid, and the Cochrane Library were searched for potential studies published from January 1, 2000 to September 30, 2019. Studies that have reported the number of NAFLD or NASH patients with normal and abnormal ALT value were included and analyzed. Abstracts, reviews, case reports, and letters were excluded. Results: A total of 11 studies with 4084 patients were included for assessing the summarized proportion of NAFLD patients with normal ALT in overall NAFLD patients. As the results shown, the summarized proportion of NAFLD patients with normal ALT value in overall NAFLD patients was 25% (95%CI: 20%-31%) which was calculated by the random-effects model. The summarized proportion of NASH patients with normal ALT value in overall NASH patients was 19% (95%CI: 13%-27%). Subgroup analysis includes region, study type, diagnostic method, and group size were conducted to investigate the resource of heterogeneity in the summarized proportion of NAFLD and NASH patients with normal ALT value. Conclusions: 25% NAFLD patients and 19% NASH patients possess the normal ALT value in the clinical manifestation. The value of ALT in the clinical diagnosis of NAFLD and NASH remains need be further testified.
WITHDRAWN: Physiological and molecular changes to short and prolonged heat in highlands China potato genotype
Authors: Liu Bailin , Cao Minxuan , Kong Lingshuang , Chen Qin , Liao Yuncheng 
Published: January 2020

Abstract
Background: Potato is the fourth world's most important crops. Global warming has heavily constrained potato production. Although some work has been undertaken regarding the response of potato to moderately increased temperature (25-30°C), few studies have examined the extreme high temperature above 35°C and sustaining high temperature impact on physiological, biochemical and molecular responses of potato. Methods: Potato plants were subjected to high temperature (35°C/33 °C day/night) treatments for 6 h (short) and 3 days (long), physiological and biochemical response of electrolyte leakage and photosynthetic performance were measured, transcriptome and metabolome profiles of leaves were examined. Expression profiles of 20 DEGs were verified by RT–qPCR, heat induced conserved genes were transient expressed in Nicotiana benthamiana.Results: Growth at short heat stress induced stomata open and lower membrane stability. Prolonged heat stress decreased the photosynthetic parameters and increased photosynthetic pigments. Integration of transcriptomics and metabolomics methods demonstrated that 448 heat upregulated and 918 heat downregulated genes as well as 325 and 219 compounds in the positive and negative ionization modes, respectively, that were up- or down-regulated in leaves detected in responsive to short and prolonged heat stress. Global transcripts changes were mainly induced by short heat stress, where metabolites changes were mainly activated by prolonged heat stress. General responses to heat stress in gene expression and metabolite accumulation enriched in amino acid metabolism and secondary metabolism pathway. Metabolite and transcript abundances for the up-regulation of flavone and flavonol biosynthesis under the prolonged heat stress were closely correlated. Both conserved and heat- and potato-specific stress responsive genes were identified by comparing heat and drought stress in potato as well as heat stress in potato and Arabidopsis shoots, transient expression of four heat induced genes in Nicotiana benthamiana exhibited heat tolerance to higher temperature.Conclusions: A new potato leaf transcriptomes and metabolomes revealed a widely adaptive response to high temperature by mainly generation and accumulation of heat shock proteins.
The Impact of an Inpatient Treatment on the Psychodynamic and Symptomatology in Couples Concordant for Substance Use: A Qualitative Study
Authors: Hansen Aksel , Brokatzky Stefan , Thomas Kai , Sternbauer Christina , Rudaz Myriam , Dammann Gerhard 
Published: January 2020

Abstract
Background: Much literature is devoted to describing the psychosocial situation of patients with a substance use disorder and the course of their families and healthy partners. Couples where both partners have a substance use disorder are less well described and even less often studied. Method: This study described the psychodynamic and symptomatology, as measured by the Brief Symptom Inventory (BSI), of five concordant couples treated simultaneously as inpatients at the same specialized substance use disorder ward. Results: The psychodynamic characterization of the couples revealed that the female was often the organizer, whereas the male was either the motivator or the lackey. In addition, the female was often emotionally dependent on the male. Most individuals showed symptom reductions from pre- to post-treatment, although the men seemed to benefit to a greater extent than the women. Moreover, the patients did not necessarily desire couples therapy. Conclusion: Treating couples concordant for substance use together in the same inpatient setting is unusual, but possible. The dynamics of couples in drug rehabilitation should be further investigated taking into account possible gender differences.
Metropolitan age-specific mortality trends at borough and neighbourhood level: The case of Mexico City
Authors: Baca-Lopez Karol , Fresno Cristobal , Espinal-Enriquez Jesus , Flores-Merino Miriam V. , Camacho-Lopez Miguel A. , Hernandez-Lemus Enrique 
Published: January 2020

Abstract
Background Understanding the spatial and temporal patterns of mortality rates in a highly inhomogeneous metropolis, is a matter of public policy interest. In this context, there is no previous study that correlates both spatio-temporal and age-specific mortality rates in Mexico City. Methods Spatio-temporal kriging modelling was used over six age-specific mortality rates (from the years 2000 to 2016 in Mexico City), to gain both spatial (borough and neighbourhood) and temporal (year and trimester) data level description. Resulting data were modelled using time-evolution mixed effect models to unblurred mortality age-specific patterns. Posterior tests were carried out to compare mortality averages between geospatial locations. Results Mortality correlation extends in all study groups for as long as 12 years and as far as 13.27 km. The highest mortality rate takes place in the Cuauhtémoc borough, as it is the commercial, touristic and cultural core downtown Mexico City. On the contrary, Tlalpan borough is the one with the lowest mortality rates in all the study groups. Interestingly, post-productive mortality is the first age-specific cause of death, followed by infant, productive, pre-school and scholar groups. Conclusion The combinations of spatio-temporal Kriging estimation and time-evolution mixed effect models, allowed us to unveil relevant time and location trends that may be useful for public policy planning in Mexico City.
Decreasing incidence of pregnancy among HIV-positive adolescents in a large HIV treatment program in western Kenya between 2005 and 2017: A retrospective cohort study.
Authors: Millar Heather Christine , Keter Alfred K. , Musick Beverly S. , Apondi Edith , Wachira Juddy , MacDonald Katherine R. , Spitzer Rachel F. , Braitstein Paula 
Published: January 2020

Abstract
Background: The objective of this study was to estimate the prevalence, incidence and risk factors for pregnancy among HIV-positive adolescents in a large HIV treatment program in western Kenya. Methods: The Academic Model Providing Access to Healthcare (AMPATH) program is a partnership between Moi University, Moi Teaching and Referral Hospital and a consortium of 11 North American academic institutions. AMPATH currently provides care to 85,000 HIV-positive individuals in western Kenya. Included in this analysis were adolescents aged 10-19 enrolled in AMPATH between January 2005 and February 2017. Socio-demographic, behavioural, and clinical data at baseline and time-updated antiretroviral treatment (ART) data were extracted from the electronic medical records and summarized using descriptive statistics. Follow up time was defined as time of inclusion in the cohort until the date of first pregnancy or age 20, loss to follow up, death, or administrative censoring. Adolescent pregnancy rates and associated risk factors were determined. Results: There were 8565 adolescents eligible for analysis. Median age at enrolment in HIV care was 14.0 years. Only 17.7% had electricity at home and 14.4% had piped water, both indicators of a high level of poverty. 12.9% (1104) were pregnant at study inclusion. Of those not pregnant at enrolment, 5.6% (448) became pregnant at least once during follow-up. Another 1.0% (78) were pregnant at inclusion and became pregnant again during follow-up. The overall pregnancy incidence rate was 21.9 per 1000 woman years or 55.8 pregnancies per 1000 women. Between 2005 and 2017, pregnancy rates have decreased. Adolescents who became pregnant in follow-up were more likely to be older, to be married or living with a partner and to have at least one child already and less likely to be using family planning. Conclusions: A considerable number of these HIV-positive adolescents presented at enrolment into HIV care as pregnant and many became pregnant as adolescents during follow-up. Pregnancy rates remain high but have decreased from 2005 to 2017. Adolescent-focused sexual and reproductive health and ante/postnatal care programs may have the potential to improve maternal and neonatal outcomes as well as further decrease pregnancy rates in this high-risk group.
Modeling the Impact of Climate Change on Hydrological Responses in the Lake Tana Basin, Ethiopia
Authors: Teklay Achenafi , Dile Yihun T. , Asfaw Dereje H. , Bayabil Haimanote K , Sisay Kibruyesfa 
Published: January 2020

Abstract
BackgroundHydrologic systems have been changing due to the impact of climate change and climate variability. The impacts of climate change are set to increase in the future due to the rise of global warming. Quantifying the impact of climate change on the spatial and temporal hydrological processes is important for integrated water resource management. The Lake Tana basin, which is the source of the Upper Blue Nile, is vulnerable to climate change and variability. This study was carried out in the four major tributary watersheds of the Lake Tana basin: Gilgel Abay, Gumara, Ribb, and Megech. The climate model and hydrological model was used to (i) to evaluate the performance of the Soil and Water Assessment Tool for study watershed, (ii) to assess the future rainfall and temperature variability and change in the study watershed, and (iii) to examine the impact of climate change on future watershed hydrology. The study used dynamically downscaled climate data for the baseline (2010–2015) and future period (2046–2051) under two Representative Concentration Pathways (RCP4.5 and RCP8.5). The climate scenarios were simulated using the Weather Research and Forecasting (WRF) model, with a 4-km horizontal resolution. A linear scaling method was applied to minimize model biases. The SWAT model was used to estimate the baseline and future hydrology using the bias-corrected climate data. ResultsThe performance of the SWAT model was ‘good’ to ‘very good’ for both the calibration and validation periods, with the Nash–Sutcliffe efficiency values between 0.71 to 0.92. The projected changes in rainfall vary with seasons and watershed under both scenarios. On average, annual rainfall may increase by 9.8% and 21.2% under RCP4.5 and RCP8.5 scenarios, respectively. Minimum temperature may rise by 1.68 °C and 2.26 °C while maximum temperature may increase by 1.65 °C and 2.75 °C under RCP4.5 and RCP8.5 scenarios, respectively. The changes in climate may cause an increase in surface runoff by 20.9% and 46.5% under RCP4.5 and RCP8.5 scenarios, respectively, while the evapotranspiration increase by 4.7% and 12.2% under RCP4.5 and RCP8.5, respectively. ConclusionThe findings provide valuable insights to implement appropriate water management strategies to mitigate and adapt to the negative impacts of climate change and variability on the Lake Tana basin, and other regions which have similar agro-ecology.
Multi-drug-resistant diarrheagenic Escherichia coli pathotypes in pediatric patients with gastroenteritis from central Iran
Authors: Abbasi Elnaz , Mondanizadeh Mahdieh , Belkum Alex van , Ghaznavi-Rad Ehsanollah 
Published: January 2020

Abstract
Background Diarrheagenic Escherichia coli (DEC) is a significant cause of gastroenteritis and a major public health problem. This study investigates the prevalence and the antibiotic resistance patterns of DEC that were isolated from infectious diarrhea samples of pediatric patients from central Iran.Methods Pediatric diarrhea samples were collected from 230 pediatric patients visiting the hospital. E. coli pathotypes were diagnosed by using conventional culture methods and PCR. Antibiotic resistance profiles, the frequency of multi-drug resistance (MDR), and the phenotypic and genotypic characteristics of extended spectrum-β-lactamase (ESBL), AmpC and integron-associated genes were analyzed.Results Of the 230 samples of infectious diarrhea, 91 (39.5%) produced E. coli isolates. Of these, 32 cases (35.1%) were identified as DEC by culture and PCR. The frequency of the E. coli pathotypes obtained was as follows: EAEC 11/32 (34.3%), EPEC 9/32 (28.1%), ETEC 6/32 (18.7%), EIEC 3/32 (9.3%), and EHEC 3/32 (9.3%). The antibiotic resistance rates were greater for nalidixic acid (30/32; 93.7%), ampicillin (29/32; 90.6%), and tetracycline (25/32; 78.1%) than for any of the other tested antibiotics. High levels of MDR (25/32; 78.1%) and the presence of ESBL (18/32; 56.2%) and AmpC (9/32; 28.1%) were observed in the DEC isolates. The isolates showed a higher frequency of the ESBL genes [ bla TEM (18/18; 100%), bla CTX-M15 (17/18; 94.4%)], and AmpC [ bla CIT (4/9; 44.4%) and bla DHA (4/9; 44.4%)] than of the other ESBL and AmpC genes.Conclusion DEC appeared to be the second-most abundant agent of diarrhea in pediatric patients after Campylobacter jejuni , with frequent MDR and ESBL presence.