bluuebunny/crossref_metadata_2025_split huggingface dataset:
3D is a feasible and more reproducible method than 2D echocardiography for in-training echocardiographers to follow-up patients with heart failure with reduced ejection fraction
Authors: Mihaila Sorina Baldea , Velcea Andreea E , Rimbas Roxana C , Andronic Anca , Matei Lavinia , Calin Simona I , Muraru Denisa , Badano Luigi , Vinereanu Dragos 
Published: March 2020

Abstract
Background: Left ventricular volumes (LVVs) and ejection fraction (LVEF) are key elements for the evaluation and follow-up of patients with heart failure with reduced ejection fraction (HFrEF). Therefore, a feasible and reproducible imaging method to be used by both experienced and in-training echocardiographers is mandatory. Aims: To establish if, in a large echo lab, echocardiographers in-training provide feasible and more reproducible results for the evaluation of patients with HFrEF, when using three-dimensional (3DE) vs. two-dimensional echocardiography (2DE). Methods: 60 patients with HFrEF (46 males, age 58±17) underwent standard transthoracic 2D acquisitions and 3D multi-beat full-volumes of the LV. One expert-user in echocardiography (Expert), and 3 echocardiographers with different levels of training in 2DE (Beginner, Medium, and Advanced) measured the 2D LVVs and LVEFs on the same consecutive images of patients with HFrEF. Afterward, the Expert performed a one-month training in 3DE analysis of the users, and both the Expert and trainees measured the 3D LVVs and LVEF of the same patients. Measurements provided by the Expert and all trainees in echo where compared.Results: 6 patients were excluded from the study due to a poor image quality. Mean end-diastolic LVV of the remaining 54 patients was 214±75 ml with 2DE, and 233±77 ml with 3DE. Mean LVEF was 35±10% with 2DE, and 33±10% with 3DE.When compared with the Expert user, the trainees showed acceptable reproducibility of the 2DE measurements, according to their level of expertise in 2DE (ICCs ranging from 0.75 to 0.94). However, after the short training in 3DE, they provided feasible and more reproducible measurements of the 3D LVVs and LVEF than with 2DE (ICCs ranging from 0.89 to 0.97).Conclusions: 3DE is a feasible, fast-learning, and more reproducible method for the assessment of LVVs and LVEF than 2DE, regardless of the basic level of expertise in 2DE of the trainees in echocardiography. In echo labs with a wide range of experience of the staff, 3DE might be a more accurate method for the follow-up of patients with HFrEF.
Identification of autophagy-associated MicroRNAs and their prognostic significance in patients with laryngeal squamous cell carcinoma
Authors: Cao Zaizai , Ao Yinjie , Guo Yu , Zhou Shuihong 
Published: March 2020

Abstract
Background: Autophagy is a biological process that cells engulf their cytoplasmic proteins or organelles to achieve the needs of metabolic and renewal. microRNAs can affect the development of cancer by regulating cell autophagy. We aim to identify the autophagy-associated MicroRNAs in laryngeal cancer and further explore their roles in the development of cancer. Results: we finally identified 6 key autophagy-associated microRNAs (AAMRs, hsa-miR-100-5p, hsa-miR-143-3p, hsa-miR-3607-5p, hsa-miR-454-3p, hsa-miR-455-5p, hsa-miR-99a-5p) were significantly correlated with prognosis of laryngeal squamous cell carcinoma (LSCC). Pathway enrichment analysis revealed that all key AAMRs were significantly enriched in the mTOR signaling pathway. The Risk index of each sample was calculated according to the result of multivariate COX analysis. Patients with lower risk index have better survival results. The area under the ROC curve for 1-year, 3-year, and 5-year survival rates were 0.7, 0.776 and 0.78 respectively.Conclusion: We identified 6 key AAMRs, which may act as new potential therapeutic targets. A new risk index model based on AAMRs can predict the prognosis of laryngeal squamous cancer.
Does Patella Position Influence Soft Tissue Balance and Clinical Outcome in Minimally Invasive Total Knee Arthroplasty?
Authors: Liu Kaiyuan , Yang Dong , Xu Tianyang , Jiang Wenwei , Fan Lin , Li Guodong 
Published: March 2020

Abstract
Purpose: We hypothesized that patellar positioning during minimally invasive total knee arthroplasty (MIS-TKA) would affect soft tissue balance and postoperative outcome.Methods: From December 2018 to February 2019, 55 patients receiving primary MIS-TKA were enrolled. The gap-balance technique was used, with patients randomly assigned to undergo osteotomy and balance of soft tissue with the patella reduced (group A; n = 27) or with the patella subluxated (group B; n = 28). The soft tissue balance achieved with the patella reduced and subluxated were compared. Femoral prosthesis rotation, mechanical femoral axis–to–tibial axis angle, Knee Society Score (KSS), pain score, and range of flexion were compared between the groups. Follow-up was for 6 months.Results: The flexion gap and the varus angle were significantly greater after patella reduction than before reduction, but the extension joint gap and varus angle were comparable before and after patella reduction. The femoral prosthesis tended to be internally rotated in group B. Range of flexion was better in the group A than in group B at 1 month after surgery, but the differences were not significant at 3 and 6 months. KSS and pain score were comparable between the groups after surgery.Conclusion: During MIS-TKA, as far as possible, soft tissue balance should be achieved with the patella reduced; otherwise, the femoral prosthesis may be installed more internally and, after patella reduction, the flexion gap and varus would increase.
Somatotypes trajectories during adulthood and its association with Chronic Obstructive Pulmonary Disease (COPD) phenotypes
Authors: Divo Miguel J. , Oto Marta Marin , Casanova Ciro , Lopez Carlos Cabrera , de-Torres Juan P. , Marin Jose Maria , Hersh Craig , Casajus Ana Ezponda , Maguire Cherie A. , Pinto-Plata Victor , Polverino Francesca , Ross James , Demeo M.P.H. M.D. Dawn L. , Bastarrika Gorka , Silverman Edwin , Celli Barolome 
Published: March 2020

Abstract
Background Chronic obstructive pulmonary disease (COPD) comprises distinct phenotypes, all characterized by airflow limitation. Research Question We hypothesized that somatotype changes -as surrogate of adiposity- from early adulthood follow different trajectories to reach distinct phenotypes. Methods Using the validated Stunkard’s pictogram, 356 COPD patients chose the somatotype that best reflects their current body build and those at ages 18, 30, 40 and 50. An unbiased group-based trajectory modelling was used to determine somatotypes trajectories. We then compared the current COPD related clinical and phenotypic characteristics of subjects belonging to each trajectory. Results At age 18, 88% of the participants described having a lean or medium somatotype (estimated BMI between 19-23 kg·m −2 )while the other 12% a heavier somatotype (estimated BMI between 25-27 kg·m −2 ). From age 18 onwards, five distinct trajectories were observed. Four of them demonstrating a continuous increase in adiposity throughout adulthood with the exception of one, where the initial increase was followed by loss of adiposity after age 40. Patients with this trajectory were primarily females with low BMI and DLCO. A persistently lean trajectory was seen in 14% of the cohort. This group had lower FEV 1 , DLCO, more emphysema and worse BODE score thus resembling the Multiple Organ Loss of Tissue (MOLT) phenotype. Conclusions COPD patients have distinct somatotype trajectories throughout adulthood.Those with the MOLT phenotype maintain a lean trajectory throughout life. Smoking subjects with this lean phenotype in early adulthood deserve particular attention as they seem to develop more severe COPD.
Difficult-to-treat rheumatoid arthritis with respect to responsiveness to newly used biologic/targeted synthetic disease-modifying anti-rheumatic drug: a retrospective cohort study
Authors: Ochi Sae , Mizoguchi Fumitaka , Nakano Kazuhisa , Tanaka Yoshiya 
Published: March 2020

Abstract
Background Difficult-to-treat rheumatoid arthritis (dt-RA) is an emerging concept, the definition of which has been proposed based on global consensus. This study aimed to establish an evidence-based definition of dt-RA with respect to responsiveness to newly used biologic and targeted synthetic disease-modifying anti-rheumatic drugs (b/tsDMARDs). Methods A retrospective cohort study was conducted using the FIRST registry. An inadequate response to current b/tsDMARDs was defined as clinical disease activity index (CDAI) >10 at week 22 or termination of treatment within 22 weeks due to insufficient efficacy. Cut-off values were defined according to the number of past failures to DMARDs and dose of glucocorticoid. Responsiveness to newly used b/tsDMARDs were compared with respect to above- versus below- cut-off values. Hazards of treatment cessation within 22 weeks due to adverse events were also compared using the same thresholds. Results The cut-off values associated with significant differences in responsiveness to b/tsDMARD treatment were ≥ 2 failures to conventional synthetic DMARD (csDMARD) treatment and ≥ 4 failures to b/tsDMARD treatment. Three or more failures to csDMARDs and concomitant use of glucocorticoid were significantly correlated with an increased hazard ratio of infection. Further analysis using clinical variables revealed that refractoriness to ≥ 2 previous csDMARDs was weakly associated with less improvement in ESR titre, while refractoriness to ≥ 4 previous b/tsDMARDs was associated with less improvement in HAQ. For both cut-offs, significant but weak association with GH was also observed. Conclusions We propose cut-off values of ≥ 2 failures to csDMARDs and/or ≥ 4 b/tsDMARDs to define dt-RA with respect to responsiveness to use of b/tsDMARDs.
Isolation and full-genome sequencing of porcine sapelovirus strain in piglets from Hainan province, China
Authors: Zhu Hechao , Li Xinxin , Xiong Jingyi , Li Xiangmin , Chen Huanchun , Qian Ping 
Published: March 2020

Abstract
Background: Porcine sapelovirus (PSV) is a species of the genus Sapelovirus within the family Picornaviridae, which associated with facute diarrhoea, respiratory distress, reproductive failure, and severe neurological disorders in swine. The first isolate strian of PSV was reported in Hainan province, China in 2019. Results: We report the isolation, genomic sequence of PSV isolated from pig diarrhea samples. The PSV strain was correctly identified by RT-PCR, IFA, WB assays, which appeared spherical with a diameter of approximately 25 nm by TEM. We named the strain PSV HaN01-CH2019, and its full genomes were 7,551 bp nucleotides in length. Phylogenetic analysis revealed that PSV HaN01-CH2019 and Chinese HuN01 strain are related in comparing with other reference strains. Conclusions: We successfully isolated the first PSV strain in Hainan province and prepared polyclonal antibodies. It is evident that PSV infection has occurred in Hainan province, and therefore, active molecular and serological investigation is important to swine population. Moreover, veterinarians must pay attention to this diarrhoea and reinforce biosecurity measures to prevent PSV spread.
Current Translational Insights into MGMT Methylation Regulating Temozolomide Sensitivity and Resistance in Glioblastoma Multiforme
Authors: Gulati Ishmeet , Patel Harsh , Prabhakar Bala , Nair Sujit 
Published: March 2020

Background:Temozolomide is used as frontline chemotherapy in the management of glioblastoma multiforme (GBM); however, its clinical utility is limited by the occurrence of significant resistance, majorly caused due to direct DNA repair. O6- methylguanine-DNA-methyltransferase (MGMT), a DNA repair protein, mediates this direct repair pathway and reverses the activity of temozolomide.Methods:We characterize and underscore the functional relevance and molecular aspects of MGMT in the development of sensitivity/resistance to temozolomide treatment. We review early translational, as well as clinical, evidence for the role of MGMT in mediating temozolomide resistance in vitro in cell lines, in vivo in small animals as well as in GBM patients.Results:Various approaches have been delineated to mitigate MGMT-induced temozolomide resistance. The most promising means in discovery biology appears to be the co-administration of MGMT inhibitors such as O6 benzyl guanine or lomeguatrib. Surprisingly, the validation of these pharmacologic inhibitors to assess the reversal of chemoresistance by appropriately designed safety and efficacy trials in combination with temozolomide is yet to be demonstrated.Conclusions:Taken together, given the regulation of temozolomide resistance by MGMT, intermediate and late discovery groups may focus their efforts on pharmacologic inhibition of MGMT, singly or in combination with radiotherapy or immunotherapy, to combat temozolomide resistance in GBM patients. In addition, one may speculate that the combined clinical use of temozolomide with a drug regulator-approved MGMT inhibitor as well as an immune checkpoint inhibitor such as nivolumab may prove beneficial. Future studies may also investigate any inter-ethnic variability in population pharmacogenetics of MGMT and pharmacometric approaches to optimize cancer precision medicine.
Epidermolisis por chikungunya por transmisión autóctona en República Dominicana. Caso atípico y severo en un recién nacido (RN)
Authors: Gómez Alba Virgen , Chalas Maylen , Michelen Eduardo 
Published: March 2020

Introducción: chikunguya, enfermedad transmitida por la picadura del mosquito del género Aedes, suele ser asin-tomática y benigna en su evolución. Sin embargo, se han observado manifestaciones clínicas atípicas y severas en niños durante la infección por chikungunya.
Objetivo: presentar caso epidermólisis por chikungunya en RN de 24 días de edad durante la epidemia del 2014 en República Dominicana.
Caso clínico: RN de 27 días de edad con lesión inicial ampollosa en tórax que luego se generalizó correspondiendo a una epidermólisis. Se observó una buena evolución y lesiones residuales híper e hipocrómicas en piel.
KONTRIBUSI DAKWAH DALAM AKTUALISASI NILAI-NILAI EKONOMI ISLAM PADA MASYARAKAT MELAYU PEDESAAN
Authors: Harahap Ginda 
Published: March 2020

The role of dakwah nowadays strategically supports the actualization of Islamic economy values in Malay society. Dakwah is aplicable activity in persuading, calling,and motivating muslims to implement Islamic teaching in daily life. This research was conducted to obtain information about the positive contribution of dakwah activities as an instrument of Islamization in the inheritance of the Islamic economic values of the Rokan Hulu Malay ethnic group, and its application in economic transactions in contemporary Malay society. The study was conducted in Rambah District, where 90% of the majority was Malay ethnic. This research used quantitative approach by spreading quetioners in collecting data. The data analysis technique used Contingency Coefficient Correlation. Based on the results of the analysis it was found that there was contribution of da'wah activities in effort to actualize Islamic economic values but it is not significant.
ANALISIS HUBUNGAN MINAT BELAJAR DAN PRESTASI BELAJAR SISWA PADA MATA PELAJARAN AL-QUR’AN HADIST
Authors: Irawan Dedi , Saputra Rendy Rinaldy 
Published: March 2020

AbstrakPenelitian ini dilatarbelakangi rendahnya minat siswa dalam pembelajaran Al-Qur’an Hadist. Hal ini berdasarkan hasil analisis prasurvey yang menunjukkan bahwa secara umum minat siswa kelas V MI Sinar Galuh,dalam belajar Al-Qur’an Hadist tergolong sedang. Penelitian ini bertujuan untuk 1) Mengetahui hubungan antara minat belajar dan prestasi belajar siswa pada mata pelajaran Al-Qur’an Hadist ; 2) Menganalisis faktor pendukung dan faktor penghambat minat siswa dalam pembelajaran Al-Qur’an Hadist. Metode yang digunakan dalam penelitian adalah metode kuantitatif dengan pendekatan korelasional. Hasil analisis data menunjukkan bahwa: 1) terdapat korelasi positif dan signifikan antara minat belajar dan prestasi siswa ; 2) faktor yang mendukung peningkatan minat siswa diantaranya : motivasi dari orang tua, guru, dan lingkungan tempat tinggal siswa; 3) faktor penghambat dalam peningkatan minat siswa antara lain : masih kurangnya sarana dan prasarana sekolah dalam upaya meningkatkan minat siswa untuk belajar.AbstractThis research is motivated by the students’ low interest on learning Al-Qur'an and Hadist. It is based on the results of pre-survey analysis which shows that in general the students' interest on pre-survey class shows that in general the interest of the fifth grade students of Sinar Galuh MI, in learning Qur'an and Hadist is classified as moderate. This study aims to 1) Determine the relation between the students’ learning interest and their achievement in Al-Qur'an and Hadist subjects; 2) Analyze the supporting and inhibiting factors of the students’ interest in learning Al-Qur'an Hadist. The method used in this study is a quantitative method with a correlational approach. The results of data analysis show that: 1) there is a positive and significant correlation between learning interest and the students’ achievement; 2) several factors that support the students’learning interest including: motivation from parents, teachers, and environment where the students’ live; 3) inhibiting factors in increasing the students’ interest including: lack of school facilities and infrastructure to increase students' learning interest in learning.
Estudo sobre a percepção do impacto do sistema cooperativo de agricultura familiar nos municípios de Mafra, Papanduva, e Monte Castelo
Authors: Diniz Clarissa Fernandes , Scharmach Andreia Luciana Rosa 
Published: March 2020

A importância da agricultura familiar para o país vem sendo objeto de estudo e discussões tanto na esfera acadêmica como em movimentos sociais e governamentais. No estado de Santa Catarina estima- se que a agricultura familiar representa um universo de 180 mil famílias, ou seja, mais de 90% da população rural, sendo responsável por mais de 70% do valor da produção agrícola e pesqueira do estado. Inúmeros produtores estão buscando o associativismo como uma maneira de fortalecer a agricultura familiar e a pequena propriedade. Este estudo tem como objetivo examinar, através da aplicação de questionários junto aos associados de cooperativas de agricultura familiar do planalto norte catarinense, a percepção do impacto do sistema cooperativo e a relação entre o produtor e a cooperativa. Através da pesquisa de campo, evidenciou- se que a cooperativa possui um papel importante na agricultura familiar, pois para o produtor ela é uma segurança de que sua produção será comercializada e vendida por um preço melhor se comparado à venda individual. Outro aspecto constatado, é que a cooperativa está atendendo as expectativas dos associados, através da melhoria de sua renda e do trabalho diário.
Mikroorganizmy zasiedlające kultury in vitro gloriozy (Gloriosa rothschildiana O’Brien) i czosnków ozdobnych (Allium L.)
Authors: KRÓL EWA , KOZAK DANUTA , PARZYMIES MARZENA , MARCINEK BARBARA , ŚWISTOWSKA ALICJA 
Published: March 2020

Podczas mikrorozmnażania roślin ozdobnych obserwowano zanieczyszczenia pożywki oraz mikrobulw gloriozy i mikropędów czosnków. W celu poznania mikroorganizmów zagrażających kulturom in vitro przeprowadzono identyfikację grzybów i bakterii na podstawie cech morfologicznych oraz wybranych testów fizjologicznych i biochemicznych. W przypadku bakterii przeprowadzono także testy patogeniczności, aby w pełni ocenić ich szkodliwość. Okazało się, że najczęściej izolowano niepatogeniczne bakterie, wśród których dominowały Gram-ujemne należące do rodzajów Pseudomonas, Enterobacter, Citrobacter, Klebsiella i Erwinia. Jednak 5 pektolitycznych izolatów Erwinia (Pectobacterium) wyizolowanych z mikropędów czosnków uznano za patogeniczne, bowiem powodowały zgniliznę pędów badanych taksonów tej rośliny. Do najczęściej izolowanych grzybów należały gatunki z rodzajów Sarocladium, Penicillium, Talaromyces i Cladosporium, które powszechnie występują w przyrodzie jako saprotrofy.
Środowiskowe zagrożenia zdrowotne na przykładzie wirusowego zapalenia wątroby typu C (HCV) w rejonie działania PSSE w Końskich
Authors: KOZAK AGNIESZKA , TIETZE MARIA , KIJ AGNIESZKA 
Published: March 2020

Wirusowe zapalenie wątroby jest obecnie uznawane za główne wyzwanie dla zdrowia publicznego. Długotrwały i bezobjawowy przebieg sprawia, iż jest ono trudne do wykrycia i zdiagnozowania. Celem pracy była charakterystyka występowania wirusowego zapalenia wątroby typu C wśród mieszkańców powiatu koneckiego. Badania przeprowadzono w 2017 r., objęto nimi zarchiwizowane dane liczbowe za lata 2013–2015, które uzyskano z Powiatowej Stacji Sanitarno-Epidemiologicznej w Końskich. Dane obejmowały wszystkie przypadki tego schorzenia, które zgłoszono do Stacji. Analizy odnotowanych przypadków zachorowań dokonano w zależności od wieku, płci oraz miejsca zamieszkania. W okresie prowadzenia badań na terenie działania PSSE w Końskich stwierdzono 13 zachorowań na wirusowe zapalenie wątroby typu C. Zdiagnozowani chorzy byli w wieku dojrzałym (30–59 lat). Wśród 13 chorych było 4 mieszkańców miast, pozostali pochodzili z terenów wiejskich. W odniesieniu do liczby mieszkańców ilość zachorowań w okresie badawczym na terenie działania PSSE w Końskich stanowiła 0,02–0,09%, co potwierdza niską wartość wskaźnika w porównaniu z krajami wschodnioeuropejskimi, gdzie wartość ta oscyluje między 1 a 6%.
DINAMIKA RISIKO KREDIT BANK PEMBANGUNAN DAERAH DI INDONESIA PERIODE 2011-2016
Authors: Ramli Ishak , Kristian Elson 
Published: March 2020

Tujuan penelitian adalah untuk menganalisis pengaruh variabel spesifik perbankan seperti informasi modal terhadap aktiva tertimbang menurut risiko diukur dengan rasio CAR, informasi total pinjaman terhadap total aset diukur dengan rasio LTA, informasi ukuran bank, informasi laba setelah pajak terhadap rata-rata aset diukur dengan rasio ROE, dan informasi inefisiensi bank yang diukur dengan rasio BOPO terhadap Risiko Kredit yang diukur dengan rasio NPL. Populasi yang dijadikan obyek penelitian berjumlah 26 BPD di Indonesia 2011-2016. Penelitian ini menggunakan data panel dengan jumlah data observasi sebanyak 156. Data sekunder diolah dengan metode Generalized Method of Moment. Hasil penelitian menunjukkan bahwa informasi modal terhadap aktiva tertimbang menurut risiko, laba setelah pajak terhadap rata-rata modal, dan inefisiensi berpengaruh negatif terhadap Risiko Kredit pada BPD di Indonesia. Sementara informasi total pinjaman terhadap total aset dan ukuran bank berpengaruh positif terhadap risiko kredit pada BPD di Indonesia. The purpose of this study is to analyze the effect of specific bank variables represented by CAR (Capital Adequacy Ratio), LTA (Loan to Asset) Ratio, Size, ROE (Return on Equity) Ratio, and Inefficiency on credit risk measured by NPL on Regional Development Bank in Indonesia. Population of this research taken as the object of observation amounted to 26 Regional Development Banks in Indonesia in the period 2011 - 2016. This research is using panel data with total data amounted to 156 firm-years observation. Secondary data is processed with Generalized Method of Moment. The result showed CAR, ROE, and Inefficiency have negative impact on NPL in Regional Development Bank in Indonesia. Meanwhile, LTA and Size have positive impact on NPL in Regional Development Bank in Indonesia.