bluuebunny/crossref_metadata_2025_split huggingface dataset:
Spontaneous onset of TNFα‐triggered colonic inflammation depends on functional T lymphocytes, S100A8/A9 alarmins, and MHC H‐2 haplotype
Authors: Leite Dantas Rafael , Bettenworth Dominik , Varga Georg , Weinhage Toni , Wami Haleluya Tesfaye , Dobrindt Ulrich , Roth Johannes , Vogl Thomas , Ludwig Stephan , Wixler Viktor 
Published: May 2020

AbstractRecently, we established a doxycycline‐inducible human tumor necrosis factor alpha (TNFα)‐transgenic mouse line, ihTNFtg. Non‐induced young and elderly mice showed low but constitutive expression of hTNFα due to promoter leakiness. The persistently present hTNFα stimulated endogenous pro‐inflammatory mouse mS100A8/A9 alarmins. Secreted mS100A8/A9 in turn induced the expression and release of mouse mTNFα. The continuous upregulation of pro‐inflammatory mTNFα and mS100A8/A9 proteins, due to their mutual expression dependency, gradually led to increased levels in colon tissue and blood. This finally exceeded the threshold levels tolerated by the healthy organism, leading to the onset of intestinal inflammation. Here, recombinant hTNFα functioned as an initial trigger for the development of chronic inflammation. Crossing ihTNFtg mice with S100A9KO mice lacking active S100A8/A9 alarmins or with Rag1KO mice lacking T and B lymphocytes completely abrogated the development of colonic inflammation, despite the still leaky hTNFα promoter. Furthermore, both the intensity of the immune response and the strength of immunosuppressive Treg induction was found to depend on the major histocompatibility complex (MHC) genetic composition. In summary, the onset of intestinal inflammation in elderly mice depends on at least four factors that have to be present simultaneously: TNFα upregulation, S100A8/A9 protein expression, functional T lymphocytes and genetic composition, with the MHC haplotype being of central importance. Only joint action of these factors leads to chronic intestinal inflammation, while absence of any of these determinants abrogates the development of the autoimmune disorder. © 2020 The Authors. The Journal of Pathology published by John Wiley & Sons Ltd on behalf of Pathological Society of Great Britain and Ireland.
Inactivation of airborne bacteria by plasma treatment and ionic wind for indoor air cleaning
Authors: Prehn Franziska , Timmermann Eric , Kettlitz Manfred , Schaufler Katharina , Günther Sebastian , Hahn Veronika 
Published: May 2020

AbstractAirborne bacteria are a general problem in medical or health care facilities with a high risk for nosocomial infections. Rooms with a continuous airflow, such as operation theaters, are of particular importance due to a possible dissemination and circulation of pathogens including multidrug‐resistant microorganisms. In this regard, a cold atmospheric‐pressure plasma (CAP) may be a possibility to support usual disinfection procedures due to its decontaminating properties. The aim of this study was to determine the antimicrobial efficacy of a plasma decontamination module that included a dielectric barrier discharge for plasma generation. Experimental parameters such as an airflow velocity of 4.5 m/s and microbial contaminations of approximately 6,000 colony‐forming units (cfu)/m3 were used to simulate practical conditions of a ventilation system in an operating theater. The apathogenic microorganism Escherichia coli K12 DSM 11250/NCTC 10538 and the multidrug‐resistant strains E. coli 21181 and 21182 (isolated from patients) were tested to determine the antimicrobial efficacy. In summary, the number of cfu was reduced by 31–89% for the tested E. coli strains, whereby E. coli K12 was the most susceptible strain toward inactivation by the designed plasma module. A possible correlation between the number or kind of resistances and susceptibility against plasma was discussed. The inactivation of microorganisms was affected by plasma intensity and size of the plasma treatment area. In addition, the differences of the antimicrobial efficacies caused through the nebulization of microorganisms in front (upstream) or behind (downstream) the plasma source were compared. The presence of ionic wind had no influence on the reduction of the number of cfu for E. coli K12, as the airflow velocity was too high for a successful precipitation, which would be a prerequisite for an increased antimicrobial efficacy. The inactivation of the tested microorganisms confirms the potential of CAP for the improvement of air quality. The scale‐up of this model system may provide a novel tool for an effective air cleaning process.
Breathing pattern disorders (dysfunctional breathing) characteristics and outcomes of children and young people attending a secondary care respiratory clinic
Authors: Newson Timothy P. , Elias Annie 
Published: May 2020

AbstractMethodsWe retrospectively audited the health records of 18 patients with breathing pattern disorders (BPDs), who were diagnosed in our respiratory clinic (2015‐2018), and then referred onto our dysfunctional breathing care pathway to a specialist speech and language therapist.ResultsThe age of the patients ranged from 11 to 16 years (median: 14 years, 14 female/4 male patients). Comorbidities included the following: 16 patients had asthma, 2 patients had a tracheoesophageal fistula and esophageal atresia repair, one patient suffered from chronic regional pain syndrome, and one patient had chronic fatigue syndrome. The commonest BPD was induced laryngeal obstruction (ILO) in 16 patients (exercise‐induced laryngeal obstruction [EILO] in 15). Other types of BPDs included hyperventilation syndrome in seven patients, psychogenic cough in eight, and dysphonia in one. A single BPD was present in 6 patients (mainly EILO), and two or more BPDs were present in 10 patients. Inducers of BPD symptoms were as follows: exercise in 16 patients, bullying in 3, anxiety in 14, emotions and stress in 11, weather in 3, posture in 1, and odors in 3. Significant school absenteeism was observed in most of the patients (2 weeks to 24 months). Significant delays in diagnosing BPD were noted (range: 1‐24 months and median: 6 months). Emergency calls occurred in 14 patients, with one patient presenting with ILO and comorbidity of asthma needing intubation and ventilation. A 1‐year follow‐up of patients showed that there was a complete resolution of BPD in two patients, 14 had good control of BPD with confidence in ability to control their symptoms, showing improved morbidity, two had no improvement, and one lost to follow‐up.ConclusionIt is important to recognize and treat BPD early to limit the significant morbidity that adversely impacts the quality of life.
Identification of biochemically neutral positions in liver pyruvate kinase
Authors: Martin Tyler A. , Wu Tiffany , Tang Qingling , Dougherty Larissa L. , Parente Daniel J. , Swint‐Kruse Liskin , Fenton Aron W. 
Published: May 2020

AbstractUnderstanding how each residue position contributes to protein function has been a long‐standing goal in protein science. Substitution studies have historically focused on conserved protein positions. However, substitutions of nonconserved positions can also modify function. Indeed, we recently identified nonconserved positions that have large substitution effects in human liver pyruvate kinase (hLPYK), including altered allosteric coupling. To facilitate a comparison of which characteristics determine when a nonconserved position does vs does not contribute to function, the goal of the current work was to identify neutral positions in hLPYK. However, existing hLPYK data showed that three features commonly associated with neutral positions—high sequence entropy, high surface exposure, and alanine scanning—lacked the sensitivity needed to guide experimental studies. We used multiple evolutionary patterns identified in a sequence alignment of the PYK family to identify which positions were least patterned, reasoning that these were most likely to be neutral. Nine positions were tested with a total of 117 amino acid substitutions. Although exploring all potential functions is not feasible for any protein, five parameters associated with substrate/effector affinities and allosteric coupling were measured for hLPYK variants. For each position, the aggregate functional outcomes of all variants were used to quantify a “neutrality” score. Three positions showed perfect neutral scores for all five parameters. Furthermore, the nine positions showed larger neutral scores than 17 positions located near allosteric binding sites. Thus, our strategy successfully enriched the dataset for positions with neutral and modest substitutions.
The brief daily stressors screening tool: An introduction and evaluation
Authors: Scholten Saskia , Lavallee Kristen , Velten Julia , Zhang Xiao‐Chi , Margraf Jürgen 
Published: May 2020

AbstractThe present study introduces a short screening instrument for the measurement of experienced general daily stressors across different life domains that can be used in large‐scale studies. The brief daily stressors screening tool (BDSST) assesses the experience of general daily stressors in eight distinct life domains. General daily stressors are indicated for the past 12‐months on a five‐point Likert scale. The present study evaluates the BDSST in two successive studies. The first study was conducted in a representative German sample (n = 7,849). The second study was conducted to assess one‐month‐retest‐stability in another representative German sample (n = 1,294). The BDSST shows promising psychometric properties. It has a skewed positive distribution, internal consistency and stability are acceptable and its one‐factor structure was confirmed in a bifactor confirmatory factor analysis. The BDSST is a reliable and valid short instrument for the assessment of experienced general daily stressors in large‐scale studies and routine clinical practise. For in‐depth clinical assessment, it can be used to identify relevant life domains for further investigation.
Daucosterol from Crateva adansoniiDC (Capparaceae) reduces 7,12‐dimethylbenz(a)anthracene‐induced mammary tumors in Wistar rats
Authors: Nguedia Merline Ymele , Tueche Alain Brice , Yaya Abel Joël Gbaweng , Yadji Vincent , Ndinteh Derek Tantoh , Njamen Dieudonné , Zingue Stéphane 
Published: May 2020

AbstractThis study aimed to evaluate the in vivo anticancer effects of daucosterol which was earlier reported to possess in vitro anticancer effects. Breast tumor was induced in 30 rats using the environmental carcinogen 7,12‐dimethylbenz(a)anthracene (DMBA) while 6 control rats received olive oil (NOR). Animals with palpable tumors were randomized into five groups (n = 6) each as follows: negative control group treated with the vehicle (DMBA); positive control group treated with 5 mg/kg BW doxorubicin (DOXO + DMBA); three groups treated with daucosterol at doses of 2.5, 5, and 10 mg/kg BW (DAU + DMBA). Treatment lasted 28 days afterward, tumor (mass, volume, cancer antigen [CA] 15‐3 level and histoarchitecture), hematological and toxicological parameters were examined. The tumor volume gradually increased in the DMBA group during the 28 days, with a tumor volume gain of ∼390 cm3. Daucosterol at all doses reduced tumor volume (∼133.7 cm3 at 10 mg/kg) as well as protein, malondialdehyde (MDA), and CA 15‐3 levels compared to DMBA rats. Tumor sections in daucosterol‐treated rats showed a lower proliferation of mammary ducts with mild (5 and 10 mg/kg) to moderate (2.5 mg/kg) inflammatory responses. Moreover, it exhibited an antioxidant effect, evidenced by a significant and dose‐dependent decreased in MDA levels, as well as an increase in catalase activity compared to the DMBA group. Daucosterol showed for the first time in vivo antitumor effects that corroborate its previous in vitro effects.
Die Pestarztmaske im Deutschen Medizinhistorischen Museum Ingolstadt
Authors: Ruisinger Marion Maria 
Published: May 2020

ZusammenfassungDieser Beitrag ist Teil des Forums COVID-19: Perspektiven in den Geistes- und Sozialwissenschaften. Die Figur des Pestarztes mit der schnabelförmigen Maske ist heute die am häufigsten zitierte Bildmetapher für die Pest. Es verwundert daher nicht, dass die Pestarztmaske in der Sammlung des Deutschen Medizinhistorischen Museums in Ingolstadt zu den am meisten nachgefragten Objekten und Bildmotiven des Hauses gehört. Der Forumsbeitrag spürt der Figur des Pestarztes auf mehreren Ebenen nach: Zunächst wird anhand zeitgenössischer Text- und Bildquellen diskutiert, welche Art von Schutzkleidung zu Seuchenzeiten empfohlen wurde und welche Rolle das schnabelförmige Maskenattribut dabei spielte. Anschließend wird das Ingolstädter Exemplar unter die Lupe genommen, hinsichtlich seines Materials und seiner Machart auf Authentizität und Praktikabilität hin untersucht und mit dem Exemplar des Deutschen Historischen Museums in Berlin verglichen.Das Ergebnis: Die frühesten Belege für die Verwendung dieser speziellen Art von Schutzkleidung stammen aus Italien und Südfrankreich und datieren aus der Mitte des 17. Jahrhunderts. Für Mitteleuropa gibt es keinerlei Nachweise dafür, dass zu Pestzeiten jemals eine solche Schnabelmaske in Gebrauch gewesen wäre. Und die Pestarztmasken in Ingolstadt und Berlin? Bei kritischer Betrachtung finden sich bei beiden Masken Details, die gegen eine Verwendung als Schutzkleidung sprechen. Ob es sich dabei um ältere, historisierende Nachbauten oder bewusste Fälschungen handelt, muss offen bleiben.
Arthroscopic coronal plane syndesmotic instability has been over-diagnosed
Authors: Hagemeijer Noortje C. , Elghazy Mohamed Abdelaziz , Waryasz Gregory , Guss Daniel , DiGiovanni Christopher W. , Kerkhoffs Gino M. M. J. 
Published: May 2020

Abstract
Purpose
Ankle arthroscopy is widely used for diagnosis of syndesmotic instability, especially in subtle cases. To date, no published article has systematically reviewed the literature in aggregate to understand which instability values should be used intraoperatively. The primary aim was to systematically review the amount of tibiofibular displacement that correlates with syndesmotic instability after a high ankle sprain. A secondary aim is to assess the quality of such research.

Methods
Systematic searches of EMBASE (Ovid) and MEDLINE via PubMed, CINAHL, Web of Science, and Google Scholar were used. Inclusion criteria: studies that arthroscopically evaluated the fibular displacement at various stages of syndesmotic ligament injury. Two reviewers independently extracted data and assessed methodological quality using the Anatomical Quality Assessment (AQUA) Tool and methodological index for non-randomized studies (MINORS).

Results
Eight cadaveric studies and three clinical studies were included for review. All studies reported displacement in the coronal plane, four studies reported in the sagittal plane, and one reported findings in the rotational plane. Four cadaveric studies had a similar experimental set up and the weighted mean associated with instability in the coronal plane could be calculated and was 2.9 mm at the anterior portion of the distal tibiofibular joint and 3.4 mm at the posterior portion. Syndesmotic instability in the sagittal plane is less extensively studied, however available data from a cadaveric study suggests thresholds of 2.2 mm of posterior fibular translation when performing an anterior to posterior hook test and 2.6 mm of anterior fibular translation when performing a posterior to anterior hook test.

Conclusions
The results have concluded that the commonly used 2.0 mm threshold value of distal tibiofibular diastasis may lead to overtreatment of syndesmotic instability, and that using threshold values of 2.9 mm measured at the anterior portion of the incisura and 3.4 mm at the posterior portion may represent better cut off values. Given the ready availability of 3 mm probes among standard arthroscopic instrumentation, at the very least surgeons should use 3 mm in lieu of 2 mm probes intraoperatively.

Level of evidence
IV.
What makes nonholonomic integrators work?
Authors: Modin Klas , Verdier Olivier 
Published: May 2020

AbstractA nonholonomic system is a mechanical system with velocity constraints not originating from position constraints; rolling without slipping is the typical example. A nonholonomic integrator is a numerical method specifically designed for nonholonomic systems. It has been observed numerically that many nonholonomic integrators exhibit excellent long-time behaviour when applied to various test problems. The excellent performance is often attributed to some underlying discrete version of the Lagrange–d’Alembert principle. Instead, in this paper, we give evidence that reversibility is behind the observed behaviour. Indeed, we show that many standard nonholonomic test problems have the structure of being foliated over reversible integrable systems. As most nonholonomic integrators preserve the foliation and the reversible structure, near conservation of the first integrals is a consequence of reversible KAM theory. Therefore, to fully evaluate nonholonomic integrators one has to consider also non-reversible nonholonomic systems. To this end we construct perturbed test problems that are integrable but no longer reversible (with respect to the standard reversibility map). Applying various nonholonomic integrators from the literature to these problems we observe that no method performs well on all problems. This further indicates that reversibility is the main mechanism behind near conservation of first integrals for nonholonomic integrators. A list of relevant open problems is given toward the end.
Transjugular intrahepatic portosystemic shunt placement: portal vein puncture guided by 3D/2D image registration of contrast-enhanced multi-detector computed tomography and fluoroscopy
Authors: Meine Timo C. , Dewald Cornelia L. A. , Becker L. S. , Mähringer-Kunz Aline , Massoumy Benjamin , Maschke Sabine K. , Kirstein Martha M. , Werncke Thomas , Wacker Frank K. , Meyer Bernhard C. , Hinrichs Jan B. 
Published: May 2020

Abstract
Background
To assess the technical feasibility, success rate, puncture complications and procedural characteristics of transjugular intrahepatic portosystemic shunt (TIPS) placement using a three-dimensional vascular map (3D-VM) overlay based on image registration of pre-procedural contrast-enhanced (CE) multi-detector computed tomography (MDCT) for portal vein puncture guidance.

Materials and methods
Overall, 27 consecutive patients (59 ± 9 years, 18male) with portal hypertension undergoing elective TIPS procedure were included. TIPS was guided by CE-MDCT overlay after image registration based on fluoroscopic images. A 3D-VM of the hepatic veins and the portal vein was created based on the pre-procedural CE-MDCT and superimposed on fluoroscopy in real-time. Procedural characteristics as well as hepatic vein catheterization time (HVCT), puncture time (PT), overall procedural time (OPT), fluoroscopy time (FT) and the dose area product (DAP) were evaluated. Thereafter, HVCT, PT, OPT and FT using 3D-VM (61 ± 9 years, 14male) were compared to a previous using classical fluoroscopic guidance (53 ± 9 years, 21male) for two interventional radiologist with less than 3 years of experience in TIPS placement.

Results
All TIPS procedure using of 3D/2D image registered 3D-VM were successful with a significant reduction of the PSG (p < 0.0001). No clinical significant complication occurred. HVCT was 14 ± 11 min, PT was 14 ± 6 min, OPT was 64 ± 29 min, FT was 21 ± 12 min and DAP was 107.48 ± 93.84 Gy cm2. HVCT, OPT and FT of the interventionalist with less TIPS experience using 3D/2D image registered 3D-VM were statistically different to an interventionalist with similar experience using fluoroscopic guidance (pHVCT = 0.0022; pOPT = 0.0097; pFT = 0.0009). PT between these interventionalists was not significantly different (pPT = 0.2905).

Conclusion
TIPS placement applying registration-based CE-MDCT vessel information for puncture guidance is feasible and safe. It has the potential to improve hepatic vein catherization, portal vein puncture and radiation exposure.
Expression of Pancreatic Stone Protein is Unaffected by Trauma and Subsequent Surgery in Burn Patients
Authors: Klein Holger J. , Buehler Philipp K. , Niggemann Pia , Rittirsch Daniel , Schweizer Riccardo , Waldner Matthias , Giovanoli Pietro , Cinelli Paolo , Reding Theresia , Graf Rolf , Plock Jan A. 
Published: May 2020

AbstractBackgroundAltered levels of pro‐inflammatory markers secondary to trauma or surgery present a major problem to physicians in being prone to interfere with the clinical identification of infectious events.MethodsPatients admitted to Zurich Burn Center between May 2015 and October 2018 with burns ≥10% total body surface area (TBSA) and without infection. Longitudinal analysis of the time course of PSP and routine inflammatory biomarkers [procalcitonin (PCT), C‐reactive protein (CRP) and white blood cells (WBC)] over two days after (a) trauma with initial debridement and (b) subsequent burn surgeries was performed. The influence of TBSA, abbreviated burn severity index (ABSI), age and length of operation was investigated using a linear mixed effect regression model.ResultsSixty‐six patients (15 female) were included with a mean age of 45.5 ± 18.3 years, median TBSA of 22% (IQR 17) and mean ABSI score 6.8 ± 2.7. PSP was the only biomarker that showed no association with any of the baseline characteristics. Additionally, PSP serum levels did not change over time neither after the burn trauma (p = 0.832) nor after secondary procedures (p = 0.113), while PCT levels increased significantly after the trauma (p < 0.001). Similarly, CRP serum levels were elevated significantly after both trauma and surgery (p < 0.001), whereas WBC values demonstrated a significant decline after the trauma (p < 0.001).ConclusionEstablished biomarkers (WBC, CRP and PCT) demonstrate decisive alterations after tissue destruction caused by burn injuries and subsequent surgical interventions. The robustness of PSP serum levels toward these inflammatory insults is a quality criterion for an upcoming sepsis biomarker.
Molecular mechanisms of dendritic cell migration in immunity and cancer
Authors: de Winde Charlotte M. , Munday Clare , Acton Sophie E. 
Published: May 2020

AbstractDendritic cells (DCs) are a heterogeneous population of antigen-presenting cells that act to bridge innate and adaptive immunity. DCs are critical in mounting effective immune responses to tissue damage, pathogens and cancer. Immature DCs continuously sample tissues and engulf antigens via endocytic pathways such as phagocytosis or macropinocytosis, which result in DC activation. Activated DCs undergo a maturation process by downregulating endocytosis and upregulating surface proteins controlling migration to lymphoid tissues where DC-mediated antigen presentation initiates adaptive immune responses. To traffic to lymphoid tissues, DCs must adapt their motility mechanisms to migrate within a wide variety of tissue types and cross barriers to enter lymphatics. All steps of DC migration involve cell–cell or cell–substrate interactions. This review discusses DC migration mechanisms in immunity and cancer with a focus on the role of cytoskeletal processes and cell surface proteins, including integrins, lectins and tetraspanins. Understanding the adapting molecular mechanisms controlling DC migration in immunity provides the basis for therapeutic interventions to dampen immune activation in autoimmunity, or to improve anti-tumour immune responses.