bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bioinformatic analysis of chickpea acetohydroxyacid synthase gene
Authors: Slishchuk H. I. , Volkova N. E. , Zakharova O. O. , Korchmaryоvа A. V. 
Published: November 2019

Aim. Analysis of homologues of chickpea gene encoding acetohydroxyacid synthase, by bioinformatics methods. Methods. Alignment of nucleotide sequences, UPGMA method, Maximum Composite Likelihood method, homologous modeling of three-dimensional structure of enzyme. Results. Homologues of the acetohydroxyacid syntase gene (AHAS) of chickpea were found among representatives of different families. A certain level of conservativeness of mRNA homologues sequences of AHAS gene within the families was noted, including legumes. The distribution of clusters corresponds to the taxonomic position of the investigated plant species. The single nucleotide polymorphism C / T at position 581, potentially associated with herbicide resistance, was detected. Based on the homologous modeling results, two models of the enzyme AHAS were constructed. The replacement of C / T, which leads to the replacement of the amino acids of alanine with valine, leads to a change in the conformation in the A chain of protein. Conclusions. Marker screening of the source breeding material by «real-time» polymerase chain reaction with the developed primers and the TaqMan probe to the polymorphic region of the AHAS gene will allow differentiating the herbicide «resistant» and «tolerant» alleles of the gene for the selection of target genotypes.
Keywords: chickpea, acetohydroxyacid syntase gene, single nucleotide polymorphism, resistance to herbicides.
Identification of putative origin of Iris pumila L. karyotype
Authors: Twardovska M. O. , Andreev I. O. , Kunakh V. A. 
Published: November 2019

Aim. The study was aimed at cytogenetic analysis of Iris pumila, I. attica, and I. pseudopumila, comparative study of the karyotypes of these species, as well as identification of putative origin of I. pumila karyotype. Methods. Cytogenetic analysis of root apical meristem, determination of chromosome number in mitotic metaphase plates, anaphase analysis. Results. The chromosome numbers observed were 2n=32 for I. pumila plants from different localities in Ukraine and 2n=16 for I. attica and I. pseudopumila plants from Greece and Italy, respectively. Some of the plants were mixoploids, the smallest proportion of mixoploids was in I. pseudopumila (10.9%) and the largest in I.pumila from all studied populations (60-80%). Anaphase analysis showed the presence of chromosomal aberrations in 2.6% of cells in roots of I. pseudopumila seedlings. The highest level of structural chromosomal aberrations (9.2%) was found in root apical meristem cells of I. pumila seedlings. Conclusions. The chromosome number was established as 2n=32 for I.pumila plants and 2n=16 for I. attica and I. pseudopumila plants. The high level of mixoploidy (60–80% of mixoploid plants) and anaphase chromosomal aberrations (up to 9.2%) was found in apical meristem of I. pumila seedlings. The amphidipiloid nature of I. pumila was established; the karyotype of the species could be formed as a result of a combination of chromosome sets from hypothetical ancestral species I. attica and I. pseudopumila. Keywords: Iris pumila L., Iris attica Boiss. & Heldr., Iris pseudopumila Tineo, chromosome number, amphidiploid, mixoploidy.
Analysis of the role of tryptophan-kynurenine pathway in the life span control in Drosophila melanogaster
Authors: Gorenskaya O. V. , Navrotskaya V. V. 
Published: November 2019

Aim. To analyze life span in mutant Drosophila stocks with impaired tryptophan-kynurenine metabolism. Methods. Wild type stocks Canton-S and Oregon, stocks with mutations of the locus white: white, whiteapricot, whitesatsuma, and stocks with the mutation vermilion have been used. The average life span of imago has been determined, survival curves have been analyzed. Results. It has been shown that the average life span of Drosophila females with mutant alleles of the white gene does not differ from the wild-type stock; in males of the w(C-S) and wa(C-S) stocks the index is increased. The presence of the mutantion vermilion in the genotype also increases the average life span of imago of both sexes, but in males the extension is more pronounced. Conclusions. The results suggest that aging is associated with the regulation of tryptophan-kynurenine metabolism.
Keywords: Drosophila melanogaster, life span, kynurenine pathway of tryptophan metabolism.
Distribution range of parasitic plants from Cuscuta L. and Orobanche L. genera in Ukraine
Authors: Krasylenko Yu. A. , Horiunova І. І. , Plokhovska S. H. , Borova М. М. , Pushkarova N. O. 
Published: November 2019

Aim. The current paper addresses the distribution of parasitic plants from Cuscuta L. (Convolvulacae) and Orobanche L. (Orobanchaceae) genera in Ukraine based on the analysis of the herbarium specimens from the National Herbarium of M.G. Kholodny Institute of Botany, NAS of Ukraine (KW) and literature. Methods. In order to study the places of growth of the dodder and broomrape the traditional method based on the development of literary sources and herbarium funds. Results. About 1600 samples from KW were analyzed and data is summarized in tables. Conclusions. It was found that the distribution range of these parasitic plants is attributed mostly to the Steppe natural zone of Ukraine including the Crimean Peninsula. The results will be used in further research to develop an efficient strategy of parasitic phytoquarantine species control.Keywords: biotic stress factors, parasitic plants, dodder, broomrape.
Subrepeats in 5s rDNAs as a molecular marker in Acer platanoides L. populations
Authors: Panchuk I. I. , Kasianchuk R. M. , Volkov R. A. 
Published: November 2019

Aim. To study the genetic diversity of tree species it is necessary to use only those regions of genome, which evolve at the highest rate, such as 5S rDNA. To estimate the potential of 5S rDNA to be used as a molecular marker for genogeographic studies, the molecular organization of this genomic region was compared between samples from two geogra-phically remote Bulgarian and Ukrainian populations of Norway maple, Acer platanoides. Methods. PCR amplification, cloning and sequencing. Results. It was shown that in the genome of A. platanoides the 5S rDNA sequences are highly similar. However, in the 5S rDNA intergenic spacer (IGS) of A. platanoides from the Bulgarian population three copies of GTCCGTT subrepeats are present, whereas only one copy of this sequence occurs in plants from the Ukrainian population. Except for different number of subrepeats, the 5S rDNA sequences of the Bulgarian and Ukrainian samples of A. platanoides are identical. In two taxonomically distant species, A. platanoides and A. pseudoplatanus, the region of 5S rDNA IGS, which contains the potential external elements of the promoter of RNA polymerase III, shows no difference, and therefore evolves at a lower rate than other parts of the IGS. Conclusions. The identity of the potential external promoter elements within the genus Acer supports the idea that this part of the IGS could be involved in the initiation of 5S rDNA transcription. The presence of different numbers of sub-repeats in the 5S rDNA IGS in various populations of A. platanoides makes them suitable for identification of intraspecific forms and for evaluation of the intraspecific genetic diversity of A. platanoides.
Keywords: 5S rDNA, molecular evolution, sub-repetitions, intraspecific variability, Acer.
Morphogenetic polymorphism of Trifolium repens L. in the pattern of white marking on a leaf on the territory of the city of Sumy with a different anthropogenic load
Authors: Toryanik V. M. , Mironets L. P. 
Published: November 2019

Aim. The peculiarities of morphogenetic polymorphism of Trifolium repens L. in the pattern of white marking on a leaf on the territory of the city Sumy with a different anthropogenic load. Methods. Collection of leafs was carried out in 2018 during the period of mass flowering of plants from the test sites covered with T. repens L. up to 60%, located in geographically distant territories of various neighborhoods of the city. Identification of drawings of white marking on a leaf was carried out according to the method of I.T. Paponova and P.Ya. Schwartzman, using the tables of J.L. Briubecker. Results. In the total sample of T. repens L. collected from territories of the city with a different anthropogenic load, 16 phenotypes and 14 genotypes, formed by a series of 7 alleles of gen V, were revealed. In specimens most often there are wild phenotypes. Among the mutant phenotypes dominate heterozygotes and are detected in the phenotype as double stains. The greatest variety of phenotypes is established on the territories of urban parks, the smallest – on the roadside and lawns of the highway. Conclusions. The phenotypic variability of T. repens L. on the territory of the city with a different anthropogenic stress indicates polymorphism of life strategies that allows maintaining a high adaptive potential in the local populations of the species.
Keywords: phenotypic and genotypic polymorphism, Тrifolium repens L., white marking on a leaf, anthropogenic load.
Similarity in temperament types of relatives
Authors: Etkalo E. N. , Atramentova L. A. 
Published: November 2019

Aim. The purpose of research is to evaluate the level of similarity of children and parents on the type of temperament. Methods. With the aid of personal questionnaire of Н. Aizenka’s (EPI) psychometric testing of teenagers and their parents is performed. The ratings of the association in concord vapours parents / descendants are designed. Results. At the men of first generation the most frequent they are sanguine (31 %) and choleric type of temperament (30 %), at women – melancholic (40 %). At youngster (the men of second generation) all types of temperament meet approximately with identical frequency, girl mostly choleric persons (39 %). Melancholiacs among them phlegmatic persons – 20 %, sanguine persons - 13% account for 28 %. Similarity on temperament - a share of concord pairs - most strong is expressed in vapours mother / son (45 %). Specific gravity of other concord pairs is accounted for: by the mother / daughter and father / 32 % daughter, father / son are 20%. The most strong association is noted in vapours mother / son on phlegmatic temperament (r=0.54), as well as choleric (r=0.26). Conclusions. In the association of temperament at parents and descendants parent effect is revealed. Similarity of temperament at descendants with mother to 1.5 times is stronger, than similarity of temperament at descendants with father.
Keywords: temperament, Н. Aizenka’s questionnaire (EPI), concord relatives, factor of the association.
Integral regulation of CHI3L1 gene and ERVW-1 locus expression by heparin in the gioblastoma cell lines U-87 MG and U-251 MG
Authors: Anopriyenko O. V. , Areshkov P. O. , Zhuk O. V. , Shablii V. A. , Skrypkina I. Ya. 
Published: November 2019

Aim. To study the effect of heparin, as a factor of tumor microenvironment, on the expression of ERVW-1 locus and CHI3L1 gene, involved in increasing of cancer cells metastatic potential, in U-87 MG (U87) and U-251 MG (U251) gliotblastoma cells. Methods. U87 and U251 cells were cultured with or without the addition of heparin to the cultural medium. Total cellular RNA was isolated and used for cDNA synthesis and subsequent PCR with primers to different regions of ERVW-1 and CHI3L1. Results. Both U87 and U251 cells showed high level of expression of full-length ERVW-1 RNA. But only in U251 cells inhibition of the full-length transcript by heparin was revealed. Spliced isoform hasn’t been detected in any of variants. Though the solely env gene transcript expressed at low level in both lines and was inhibited similarly by heparin. Expression of CHI3L1 has been detected in both lines with primers towards exons 4-5 with some lowering in level under the heparin influence. Only in U87 cells the PCR-fragment with primers to exons 8-9 has been detected. In control U87 cells hypothetic spliced isoform of CHI3L1 transcript has been revealed with primers to exons 1-9. Conclusions. Heparin has complex and cell line-dependent regulation of expression of ERVW-1 locus and CHI3L1 gene in the glioblastoma cell lines U87 and U251.
Keywords: glioblastoma, heparin, ERVW-1, CHI3L1.
Distribution of mistletoe (Viscum album L.), which parasitizes different woody plants species, in Kyiv and its genetic characteristics
Authors: Bilonozhko Yu. O. , Ponomarenko L. O. , Rabokon A. M. , Postovoitovа A. S. , Kalafat L. O. , Privаlikhin S. M. , Blume Ya. B. , Pirko Ya. V. 
Published: November 2019

Aim. The aim of the work was to study the species diversity of mistletoe host trees with the establishment of damage degrees and the study of the mistletoe genetic characteristics in Kiev. Methods. The β-tubulin first intron length polymorphism evaluating method (TBP) was used. Amplified fragments DNA were fractionated by electrophoresis in non-denaturing polyacrylamide gel. DNA bands were detected using silver nitrate staining. Results. The species diversity of Viscum album L. host-trees was studied in Kiev. Among them, plants of the genus Pinus and angiosperms that belong to 8 genera (Acer, Fraxinus, Acacia, Populus, Tilia, Salix, Malus, Sorbus), wherein 47% belong to the genus Acer. It was revealed that among infected trees, 30% had severe damage to mistletoe, 28% moderate and 42% weak. Based on the analysis of DNA marker polymorphisms, the molecular-genetic profiles of V. album, which grows on various species of host trees, were obtained and analyzed. Conclusions. The species diversity of V. album host-trees was studied and the degree of their infection was assessed in Kiev. Mistletoe, which grows on angiosperms, is characterized by a greater degree of genetic polymorphism than that which grows on gymnosperms.
Keywords: Viscum album L., host-plant, introns of genes, length polymorphism, β-tubulin.
Microbe-plant interactions between Streptomyces and model agricultural plants – Hordeum vulgare and Lycopersicon esculentum (Microtom)
Authors: Mutenko H. V. , Ostash B. O. , Rothballer M. , Weiss A. , Schmid M. , Hartmann A. , Fedorenko V. O. 
Published: November 2019

Aim. Microbe-plant interactions (MPI) constitute an important aspect of ecology because of their significant influence on plant’s ability to withstand abiotic stress and infection. In comparison to proteobacteria and bacilli, the roles of streptomycetes in MPI remain poorly studied. Here, we elucidate some aspects of MPI between two model plant species, Hordeum vulgare and Lycopersicon esculentum, and several strains of Streptomyces lividans 1326 and S. ghanaensis ATCC14672. Methods. Microbiology, microscopy and molecular genetics were combined to reveal the MPI. Results. We demonstrate the colonization of H. vulgare and L. esculentum roots by different strains of S. ghanaensis deficient in production of either the antibiotic moenomycin or signaling molecule of the γ-butyrolactone type. The treatment of H. vulgare seeds with S. lividans spores increased the root biomass. Plants treated with 1,4-butyrolactone had no positive influence on plants, at milimolar concentrations this compound inhibited the root and shoot growth of L. esculentum. Conclusions. Roots of two mono- and dicot plants are colonized by Streptomyces; reporter gene uidA is useful to monitor the colonization. Under our experimental conditions the ability to colonize plants by streptomycetes was not affected by the deficiency in antibiotic or butenolide production.
Keywords: Streptomyces ghanaensis, moenomycinA, low-molecular weight signal compounds, root colonization.
Non-additive expression of awn development regulatory genes in the bread wheat lines with introgressions from Amblyopyrum muticum
Authors: Navalikhina A. G. , Antonyuk M. Z. , Ternovska T. K. 
Published: November 2019

Aim. Nonadditive expression of homeotic genes is considered to cause the development of nonparental phenotypes in the plants of hybrid origin. Previously, orthologs of rice and barley awn development regulators TaTOB1, TaDL, TaKNOX3, and TaETT2 were identified in the bread wheat genome sequence. Nonadditive expression of these regulators can be the reason for the emergence of non-parental terminally awned phenotypes among the bread wheat lines with introgressions from Amblyopyrum muticum Methods. Gene expression was identified with end-point detection RT-qPCR Results. Introgressive lines have expression of TaTOB1, TaKNOX3, and TaETT2 at the lower level compared to parents. As orthologs of TaTOB1 and TaKNOX3 are negative regulators of awn development in rice and barley, their reduced expression could have caused the appearance of terminally awned plants among introgressive lines. Neverthless, the reduced expression of the genes wasn’t specific to the lines with non-parental phenotype. Conclusions. Due to the lack of correlation between reduced expression of the genes studied and non-parental phenotype of the introgressive lines, the role of nonadditive expression of TaTOB1, TaKNOX3, and TaETT2 in the development of this phenotype is not clear. Keywords: amphidiploids, non-additive expression, developmental genetics, awns.
Molecular characterization of high-molecular weight glutenin subunits 1Bx6.1 and 1By22.1 from Triticum spelta К1731 accession
Authors: Orlovskaya O. A. , Yatsevich K. K. , Vakula S. I. , Khotyleva L. V. , Kilchevsky A. V. 
Published: November 2019

Aim. Some spelt varieties, along with alleles of gliadins and high-molecular glutenin subunits (HMW-GS), identical to common wheat, contain specific alleles, that are source of Triticum aestivum gene pool enrichment. The aim of this work is the identification, molecular analysis of HMW – GS from T. spelta K1731 and evaluation of their effect on the elastic properties of gluten. Methods. Identification of HMW-GS was carried out by SDS-electrophoresis and PCR analysis. Nucleotide gene sequences were determined by Sanger sequencing. The secondary structure of proteins was predicted on the on-line CFSSP server. Results. Subunits 6.1 + 22.1 of the Glu-B1 locus encoded by the Glu-B1be allele were detected in the T. spelta K1731. The nucleotide sequences of the 1Bx6.1, 1By22.1 genes from spelt were determined, the amino acid sequence and the protein secondary structure of 6.1 + 22.1 subunits were analyzed. Conclusions. Molecular analysis of HMW-GS 1Bх6.1 and 1By22.1 from T. spelta К1731 established a low contribution to the bread-making quality of these subunits.
Keywords: Triticum spelta K1731, HMW-GS, SDS electrophoresis, sequencing, secondary protein structure, gluten quality.
Ist and IIIrd intron length polymorphism of actin genes as a tool for flax DNA-profiling
Authors: Postovoitova A. S. , Pirko Ya. V. , Blume Ya. B. 
Published: November 2019

Aim. The purpose of the work was to evaluate the possibility of using the polymorphism of the Ist and IIIrd introns of actin genes for DNA plant genotyping using flax varieties as model. Methods. 16 varieties of Ukrainian flax were analyzed. PCR was conducted using self-developed species-specific primers for the Ist and IIIrd introns of flax actin genes. DNA fragments were separated by electrophoresis in a 6% polyacrylamide gel and visualized by silver stains. Results. As a result of the evaluation of the Ist and IIIrd intron length polymorphism of actin genes, the species-specific DNA profiles of 16 flax varieties containing the target amplicons were obtained. The 7 allele phenotypes (PIC = 0.62) were detected for the Ist introns of the actin genes, and 3 allelic phenotypes (PIC = 0.32) for the IIIrd intron of actin genes. The highest level of polymorphism in the flax varieties was detected by evaluating the Ist intron length polymorphism of actin genes. Conclusions. Evaluation of the polymorphism of the Ist and IIIrd introns of actin genes allows genotyping and obtaining DNA profiles of flax varieties, which demonstrates the feasibility of further using both approaches for molecular genetic analysis of plants.
Keywords: gene introns, length polymorphism, actin genes, flax (Linum usitatissimum L.).
Polymorphism of two wheat degydrines genes
Authors: Urbanovich O. Yu. , Kuzmitskaya P. V. , Borovskiy G. B. 
Published: November 2019

Aim. The aim of this work is to study the nucleotide sequences variability of two genes encoding wheat dehydrins. One of them belong to K-2 group, the other refers to K-3 type (respectively, TaDHN18 and TaDHN19.3 according to the classification of Wang et al.). These loci sre interesting due to their's genes participation in protection of plants from the various adverse abioti ecffects. Methods. Polymorphism of two genes encoding dehydrins was investigated using 6 wheat varieties as examples, among which were spring and winter varieties with different cold resistance, using PCR-based cloning, sequencing, and data processing softwares. Results. An analysis of the obtained nucleotide and hypothetical protein sequences alignment showed that the locus under study in the genomes of different wheat varieties, both spring and winter, is characterized by a high identity degree. Conclusions. The high conservatism of two loci encoding K-2 and K-3 dehydrins was demonstrated.
Keywords: wheat, dehydrins, polymorphism.
Induction of chlorophyll fluorescence use for assessment of functioning of Gentiana lutea L. plants photosynthetic apparatus in different conditions of culturing in vitro
Authors: Hrytsak L. R. , Herts A. I. , Herts N. V. , Drobyk N. M. 
Published: November 2019

Aim. To study the peculiarities of functioning of photosynthetic apparatus of Gentiana lutea L. plants in vitro under different light qualities and source of carbon in the composition of nutrient medium by using the induction of chlorophyll fluorescence (ICF) method. Methods. Chlorophyll fluorescence was determined in light-adapted leaves of cultivated in vitro G. lutea plants by use of PAM fluorometry MultispeQ. The parameters change of photosynthetic apparatus functioning of cultivated in vitro plants was assessed with regard to light qualities (Variant 1: light intensity – 85 W/m2, when different spectra combinations of blue-wave band (Еb) and green-wave band (Еg) and red-wave band (Еr) was 33% : 42% : 25%; Variant 2: light intensity – 100 W/m2, with wave bands of spectra – Еb : Еg : Еr = 25% : 27% : 48%) and the source of carbon (10 g/l of sucrose or 3 g/l of mannite) in the composition of nutrient medium МS/2 (MS medium with half amount of macro- and microsalts), supplemented with 0.1mg/l of kinetin. Results. It was established that G. lutea plants cultivated in vitro for 90 days in light conditions of Variant 2 had a quantum yield photochemical of PS ІІ 8.3 % higher in comparison to G. lutea specimens cultured in light conditions of Variant 1. The vitality value of Variant 2 plants was 23 % higher. Change of carbon source in the composition of nutrient medium from sucrose (10 g/l) to mannite (3 g/l) was both increasing the efficiency of functioning of photosynthetic apparatus of Gentiana lutea L. plants in vitro and activating their resistance mechanisms to water deficit. Conclusions. The ICF method use shows that functioning of photosynthetic apparatus of G. lutea plants in vitro depends different qualities light and source of carbon in the composition of nutrient medium.
Key words: induction of chlorophyll a fluorescence, plants in vitro, Gentiana lutea L.