bluuebunny/crossref_metadata_2025_split huggingface dataset:
Heterobimetallic Ru(ii)/Fe(ii) complexes as potent anticancer agents against breast cancer cells, inducing apoptosis through multiple targets
Authors: Guedes Adriana Pereira Mundim , Mello-Andrade Francyelli , Pires Wanessa Carvalho , de Sousa Maria Alice Montes , da Silva Paula Francinete Faustino , de Camargo Mariana S , Gemeiner Hendryk , Amauri Menegário A , Gomes Cardoso Clever , de Melo Reis Paulo Roberto , Silveira-Lacerda Elisângela de Paula , Batista Alzir A 
Published: February 2020

Abstract
Antimetastatic activity, high selectivity and cytotoxicity for human tumor cell lines make ruthenium(ii) complexes attractive for the development of new chemotherapeutic agents for cancer treatment. In this study, cytotoxic activities and the possible mechanism of cell death induced by three ruthenium complexes were evaluated, [Ru(MIm)(bipy)(dppf)]PF6 (1), [RuCl(Im)(bipy)(dppf)]PF6 (2) and [Ru(tzdt)(bipy)(dppf)]PF6 (3). The results showed high cytotoxicity and selectivity indexes for the human triple-negative breast tumor cell line (MDA-MB-231) with IC50 value and selectivity index for complex 1 (IC50 = 0.33 ± 0.03 μM, SI = 4.48), complex 2 (IC50 = 0.80 ± 0.06 μM, SI = 2.31) and complex 3 (IC50 = 0.48 ± 0.02 μM, SI = 3.87). The mechanism of cell death induced in MDA-MB-231 cells, after treatment with complexes 1–3, indicated apoptosis of the cells as a consequence of the increase in the percentage of cells in the Sub-G1 phase in the cell cycle analysis, characteristic morphological changes and the presence of apoptotic cells labeled with Annexin-V. Multiple targets of action were identified for complexes 1 and 3 with an induction of DNA damage in cells treated with complexes 1 and 3, mitochondrial depolarization with a reduction in mitochondrial membrane potential, an increase in reactive oxygen species levels and increased expression levels of caspase 3 and p53. In addition, antimetastatic activities for complexes 1 and 3 were observed by inhibition of cell migration by the wound healing assay and Boyden chamber assay, as well as inhibition of angiogenesis caused by MDA-MB-231 tumor cells in the CAM model.
Neue Rechtslage für Mahlzeitersatz und Tagesrationen
Authors: Bode Volker , Pahne Norbert 
Published: February 2020

ZusammenfassungSogenannte „Lebensmittel für eine kalorienarme Ernährung zur Gewichtsverringerung“, landläufig auch als „Formuladiäten“ bezeichnet, sind anerkannte Maßnahmen in der Gewichtsreduktion. Dazu zählen die Produktkategorien „Mahlzeitersatz“ und „Tagesrationen“, deren Anforderungen in Europa und so auch in Deutschland im Detail vorgeschrieben sind. Bisher waren diese Produkte im europäischen Diätrecht bzw. in der deutschen Diätverordnung (DiätV) genau geregelt. Mit der Revision des europäischen Diätrechts, welche 2011 eingeläutet wurde, wurde das Konzept der „diätetischen Lebensmittel“ durch das Konzept für „Lebensmittel für spezielle Verbrauchergruppen“ – sogenannte FSG-Produkte – ersetzt. Diese Neuregelungen betreffen auch im vollen Umfang „Lebensmittel für eine kalorienarme Ernährung zur Gewichtsverringerung“. Für Mahlzeitersatz und Tagesrationen ergeben sich damit gravierende Änderungen hinsichtlich Zusammensetzung, Verwendungsdauer sowie Zielgruppen.Für Tagesrationen wurden neue und detaillierte Anforderungen über einen delegierten Rechtsakt festgelegt. Darin wurde die Zielgruppe der Produkte neu festgelegt, auch gibt es maßgebliche Änderungen hinsichtlich des Makro- und Mikronährstoffgehalts. Zudem sind zukünftig keine gesundheits- und nährwertbezogenen Angaben mehr möglich. Produkte, die zur Kategorie Mahlzeitersatz gehören, sollen nur noch als „Lebensmittel des allgemeinen Verzehrs“ angesehen werden. Damit wird der Verbund dieser beiden Produktgruppen aufgelöst. Mahlzeitersatzprodukte erfahren eine deutliche Einschränkung, da der Begriff Mahlzeit neu definiert wurde. Auch ergeben sich deutliche Änderungen bei den Mikronährstoffen. Des Weiteren verbleiben erhebliche Unsicherheiten hinsichtlich des Einsatzes von Zusatzstoffen. Neben den technologischen Herausforderungen für die Hersteller, ist auch die Wirtschaftlichkeit dieser Produkte zu prüfen. Im Rahmen der konservativen Adipositastherapie wäre eine Aufgabe dieser Produkte ein großer Verlust für Ärzte und Patienten. Daher ist es von entscheidender Bedeutung, dass sich die Fachkreise für diese Produktgruppen einsetzen.
Arteriosklerose und Koronare Herzerkrankung
Authors: Bös Dorothee 
Published: February 2020

ZusammenfassungHeilpilze werden aufgrund ihrer wertvollen therapeutisch wirksamen Inhaltsstoffe als „Medicinal Mushrooms“ zunehmend erforscht und eingesetzt. Insbesondere in der Behandlung der zahlreichen Zivilisationserkrankungen haben sich gute Anwendungsergebnisse gezeigt. Die Mykotherapie bei Arteriosklerose/KHK setzt sehr vielschichtig und bei beinahe allen Ursachen an. Zudem können mit der richtigen Heilpilzauswahl Begleitsymptome therapeutisch berücksichtigt werden.Der Beitrag gibt einen Überblick zu den wichtigsten Heilpilzen bei Arteriosklerose und KHK.
Ni-Catalyzed Reductive Difunctionalization of Alkenes
Authors: Ping Yuanyuan , Kong Wangqing 
Published: February 2020

Alkene difunctionalization represents one of the most efficient methods to synthesize highly functionalized molecules from simple and readily available starting materials. In contrast to the well-established redox-neutral alkene difunctionalization reactions, reductive alkene difunctionalization, which simultaneously introduces two electrophiles on both sides of the double bond, has been much less developed, especially in enantioselective manner. This review summarizes recent advances in the nickel-catalyzed reductive difunctionalization of alkenes and highlights the enantioselective transformations.1	Introduction2	Nickel-Catalyzed Racemic Reductive Difunctionalization of Alkenes3	Nickel-Catalyzed Enantioselective Reductive Difunctionalization of Alkenes3.1	Diarylation of Alkenes3.2	Aryl-alkenylation of Alkenes3.3	Aryl-monofluoroalkenylation of Alkenes3.4	Reductive Cyclization/Coupling with Alkynyl Bromides or Asymmetric Internal Alkynes3.5	Aryl-alkylation of Alkenes3.6	Aryl-amination of Alkenes4	Summary and Outlook
Promoting Quality Face-to-Face Communication during Ophthalmology Encounters in the Electronic Health Record Era
Authors: Baxter Sally L. , Gali Helena E. , Chiang Michael F. , Hribar Michelle R. , Ohno-Machado Lucila , El-Kareh Robert , Huang Abigail E. , Chen Heather E. , Camp Andrew S. , Kikkawa Don O. , Korn Bobby S. , Lee Jeffrey E. , Longhurst Christopher A. , Millen Marlene 
Published: February 2020

Abstract
Objective To evaluate informatics-enabled quality improvement (QI) strategies for promoting time spent on face-to-face communication between ophthalmologists and patients.
Methods This prospective study involved deploying QI strategies during implementation of an enterprise-wide vendor electronic health record (EHR) in an outpatient academic ophthalmology department. Strategies included developing single sign-on capabilities, activating mobile- and tablet-based applications, EHR personalization training, creating novel workflows for team-based orders, and promoting problem-based charting to reduce documentation burden. Timing data were collected during 648 outpatient encounters. Outcomes included total time spent by the attending ophthalmologist on the patient, time spent on documentation, time spent on examination, and time spent talking with the patient. Metrics related to documentation efficiency, use of personalization features, use of team-based orders, and note length were also measured from the EHR efficiency portal and compared with averages for ophthalmologists nationwide using the same EHR.
Results Time spent on exclusive face-to-face communication with patients initially decreased with EHR implementation (2.9 to 2.3 minutes, p = 0.005) but returned to the paper baseline by 6 months (2.8 minutes, p = 0.99). Observed participants outperformed national averages of ophthalmologists using the same vendor system on documentation time per appointment, number of customized note templates, number of customized order lists, utilization of team-based orders, note length, and time spent after-hours on EHR use.
Conclusion Informatics-enabled QI interventions can promote patient-centeredness and face-to-face communication in high-volume outpatient ophthalmology encounters. By employing an array of interventions, time spent exclusively talking with the patient returned to levels equivalent to paper charts by 6 months after EHR implementation. This was achieved without requiring EHR redesign, use of scribes, or excessive after-hours work. Documentation efficiency can be achieved using interventions promoting personalization and team-based workflows. Given their efficacy in preserving face-to-face physician–patient interactions, these strategies may help alleviate risk of physician burnout.
Infinite transitivity for Calogero-Moser spaces
Authors: Kuyumzhiyan Karine 
Published: February 2020

We prove the conjecture of Berest-Eshmatov-Eshmatov by showing that the group of automorphisms of a product of Calogero-Moser spaces

C

n
i

\mathcal {C}_{n_i}

, where the

n
i

n_i

are pairwise distinct, acts

m
m

-transitively for each

m
m

.
Nutrition in the context of the Sustainable Development Goals
Authors: Grosso Giuseppe , Mateo Alberto , Rangelov Natalie , Buzeti Tatjana , Birt Christopher 
Published: February 2020

Abstract
The 2030 Agenda for the Sustainable Development Goals (SDGs) represents a common framework of international cooperation to promote sustainable development. Nutrition is the key point for the SDG 2 ‘End hunger, achieve food security and improved nutrition and promote sustainable agriculture’ and is an essential component for achieving many of the other targets: overall, the nutritional aspects of the SDGs aim to promote healthy and sustainable diets and ensure food security globally. While undernutrition is of minimal concern in the European Union Member States, trends in childhood obesity are still alarming and far from any desirable target. European food production systems have improved over the last years, with immediate impact on several environmental aspects; however, a comprehensive regulatory framework to fulfil the environmental and climate targets is still lacking. Policy actions at multinational level are needed to achieve global nutrition targets designed to guide progress towards tackling all forms of malnutrition while preserving the environment through virtuous food production and food systems.
A minimum set of regulators to thrive in the ocean
Authors: Lambrecht S Joke , Steglich Claudia , Hess Wolfgang R 
Published: February 2020

ABSTRACTMarine cyanobacteria of the genus Prochlorococcus thrive in high cell numbers throughout the euphotic zones of the world's subtropical and tropical oligotrophic oceans, making them some of the most ecologically relevant photosynthetic microorganisms on Earth. The ecological success of these free-living phototrophs suggests that they are equipped with a regulatory system competent to address many different stress situations. However, Prochlorococcus genomes are compact and streamlined, with the majority encoding only five different sigma factors, five to six two-component systems and eight types of other transcriptional regulators. Here, we summarize the existing information about the functions of these protein regulators, about transcriptomic responses to defined stress conditions, and discuss the current knowledge about riboswitches, RNA-based regulation and the roles of certain metabolites as co-regulators. We focus on the best-studied isolate, Prochlorococcus MED4, but extend to other strains and ecotypes when appropriate, and we include some information gained from metagenomic and metatranscriptomic analyses.
Satellite imaging of the 2015 M7.2 earthquake in the Central Pamir, Tajikistan, elucidates a sequence of shallow strike-slip ruptures of the Sarez-Karakul fault
Authors: Elliott A , Elliott J , Hollingsworth J , Kulikova G , Parsons B , Walker R 
Published: February 2020

SUMMARYOn 7 December 2015, a shallow Mw 7.2 strike-slip earthquake struck the Murghab River Valley in the Central Pamirs of Tajikistan. Seismologically this event was similar to a large seismic event in 1911 whose causative fault has never been identified. We measure the displacement field of the 2015 event from satellite observations using Sentinel-1 radar interferometry, Landsat-8 optical pixel-tracking, and surface rupture mapping from high resolution SPOT-6/7 imagery to characterize the role this earthquake rupture plays in the accommodation of strain on its causative structure, the Sarez-Karakul fault. We present geomorphic mapping and interpretations of other Quaternary-active reaches of this fault system, which highlight variable rupture history of the different sections. These sections appear to be separated by inherited bedrock structural boundaries. Significantly, the reaches of the fault northeast and southwest of the 2015 rupture exhibit the freshest morphology prior to 2015, indicative of a more recent rupture than elsewhere. Using new high resolution imagery we map fresh scarps at the northern and southern ends of the Sarez-Karakul fault which may represent this 1911 rupture. To test which of these reaches could have been the source of the elusive 1911 event, we compare synthetic seismograms from three plausible fault sources determined from geomorphology, with observed seismic traces from 1911 at early recording stations throughout Europe. We find that the best fitting fault source is in fact southwest of the 2015 rupture, meaning that we have a record of three distinct recent events on the Sarez-Karakul fault system—two of them instrumentally recorded. Our mapping of these separate events reveals a correlation between their boundaries and the active and inherited thrust and suture systems that intersect the northeast striking left-lateral fault, suggesting structural control over the extents of individual ruptures on the active strike-slip fault.
The HOPS complex subunit VPS39 controls ciliogenesis through autophagy
Authors: Iaconis Daniela , Crina Claudia , Brillante Simona , Indrieri Alessia , Morleo Manuela , Franco Brunella 
Published: February 2020

Abstract
Primary cilia are microtubule-based organelles that assemble and protrude from the surface of most mammalian cells during quiescence. The biomedical relevance of cilia is indicated by disorders ascribed to cilia dysfunction, known as ciliopathies, that display distinctive features including renal cystic disease. In this report, we demonstrate that vacuolar protein sorting 39 (VPS39), a component of the homotypic fusion and vacuole protein sorting (HOPS) complex, acts as a negative regulator of ciliogenesis in human renal cells, by controlling the localization of the intraflagellar transport 20 protein at the base of cilia through autophagy. Moreover, we show that VPS39 controls ciliogenesis through autophagy also in vivo in renal tubules of medaka fish. These observations suggest a direct involvement of the HOPS complex in the regulation of autophagy-mediated ciliogenesis and eventually in target selection. Interestingly, we show that the impact of autophagy modulation on ciliogenesis is cell-type dependent and strictly related to environmental stimuli. This report adds a further tile to the cilia-autophagy connection and suggests that VPS39 could represent a new biological target for the recovery of the cilia-related phenotypes observed in the kidneys of patients affected by ciliopathies.
Restructuring Euro Area Sovereign Debt: Have the Options Narrowed?
Authors: Weidemaier W Mark C 
Published: February 2020

ABSTRACT
This article examines the intersection between two key attributes of sovereign debt governance in the Euro Area. First, sovereigns mostly issue bonds governed by their own law. This ‘local law advantage’ should make debt restructuring comparatively easy, as the sovereign can change the law to reduce its debt. The second attribute is the so-called ‘Euro CAC’, which is a contract-based restructuring mechanism mandated by the Treaty Establishing the European Stability Mechanism (the ESM Treaty). The Euro CAC lets a bondholder supermajority approve a restructuring and bind dissenters. Since 2013, nearly all Euro Area sovereign debt has included the clause. Many believe the ESM Treaty requires governments to use the Euro CAC to restructure. But if so, the Treaty is a suicide pact, for the design of the Euro CAC is flawed. In a meaningful subset of cases, the clause will not provide adequate debt relief. This article makes two primary contributions. First, using an Italian restructuring as an example, it explains why the ESM Treaty does not, in fact, require the use of the Euro CAC. Second, it examines the legal constraints—the most pertinent of which derive from the European Convention on Human Rights—that do restrict the use of local-law advantage.
Bayesian Semi-Parametric Realized Conditional Autoregressive Expectile Models for Tail Risk Forecasting
Authors: Gerlach Richard , Wang Chao 
Published: February 2020

AbstractA new model framework called Realized Conditional Autoregressive Expectile is proposed, whereby a measurement equation is added to the conventional Conditional Autoregressive Expectile model. A realized measure acts as the dependent variable in the measurement equation, capturing the contemporaneous dependence between it and the latent conditional expectile; it also drives the expectile dynamics. The usual grid search and asymmetric least squares optimization, to estimate the expectile level and parameters, suffers from convergence issues leading to inefficient estimation. This article develops an alternative random walk Metropolis stochastic target search method, incorporating an adaptive Markov Chain Monte Carlo sampler, which leads to improved accuracy in estimation of the expectile level and model parameters. The sampling properties of this method are assessed via a simulation study. In a forecast study applied to several market indices and asset return series, one-day-ahead Value-at-Risk and Expected Shortfall forecasting results favor the proposed model class.
The Health and Readiness of Service Members: ACEs to PACEs
Authors: Lamson Angela , Richardson Natalie , Cobb Erin 
Published: February 2020

ABSTRACT

Introduction
Over the past three decades, a growing research base has emerged around the role of adverse childhood experiences (ACEs) in the biological, psychological, social, and relational health and development of children and adults. More recently, the role of ACEs has been researched with military service members. The purpose of this article was to provide a brief description of ACEs and an overview of the key tenets of the theory of toxic stress as well as a snapshot of ACEs and protective and compensatory experiences (PACEs) research with active duty personnel.

Methods
Ninety-seven active duty personnel completed the study including questions pertaining to demographics, adverse childhood experiences, adult adverse experiences, and PACEs survey.

Results
Significant findings pertaining to ACEs and PACEs were found by service member’s sex and rank, with higher ACE scores for men and enlisted service members.

Conclusions
The contrast by rank and sex in relation to ACEs punctuates the need for attention to ACEs and protective factors among early career service members in order to promote sustainable careers in the military.
Continuity in Psychological Health and Role Functioning Across Deployment
Authors: Southwell Kenona H , Coppola Elizabeth C , Topp David B , MacDermid Wadsworth Shelley M 
Published: February 2020

ABSTRACT

Introduction
Scholars have described military deployments as one of the most stressful aspects of life for military couples. Deployment affects multiple roles and family members, yet little is known about the degree to which postdeployment outcomes are accounted for by predeployment functioning independent of deployment experiences.

Methods
Data collection included in-person interviews with National Guard couples experiencing a deployment and a comparison group whose deployment was canceled abruptly. Using hierarchical regression, this study assessed (a) how much variance in postdeployment functioning was explained by predeployment functioning and (b) whether variance accounted for by predeployment functioning differed by domain, respondent, or deployment status. Posthoc analyses revealed which combinations of predeployment functioning accounted for the most variance in postdeployment outcomes.

Results
We found evidence of modest continuity between predeployment and postdeployment functioning, particularly for psychological functioning and partner role functioning, and fewer differences than expected in patterns between groups. Certain demographic characteristics, risk factors, and resources accounted for significant variance in postdeployment outcomes in addition to baseline levels of role functioning.

Conclusions
Study findings reinforce the importance of predeployment preparation, providing families with resources to maximize resilience in response to the stress of deployment.
The Effectiveness of the DoD/VA Mobile Device Outcomes-Based Rehabilitation Program for High Functioning Service Members and Veterans with Lower Limb Amputation
Authors: Gaunaurd Ignacio , Gailey Robert , Springer Barbara , Symsack Allison , Clemens Sheila , Lucarevic Jennifer , Kristal Anat , Bennett Christopher , Isaacson Brad , Agrawal Vibhor , Applegate Brooks , Pasquina Paul 
Published: February 2020

Abstract

Introduction
The objective was to determine if the Mobile Device Outcomes-based Rehabilitation Program (MDORP) improved strength, mobility, and gait quality in service members (SMs) and Veterans with lower limb amputation (LLA).

Methods
Seven SMs and 10 Veterans with LLA enrolled and were trained to use a mobile sensor system, called Rehabilitative Lower Limb Orthopedic Analysis Device (ReLOAD). ReLOAD provided participants with real-time assessment of gait deviations, subsequent corrective audio feedback, and exercise prescription for normalizing gait at home and in the community. After baseline testing, prosthetic gait and exercise training, participants took ReLOAD home and completed an 8-week walking and home exercise program. Home visits were conducted every 2 weeks to review gait training and home exercises.

Results
Significant improvements in hip extensor strength, basic and high-level mobility, musculoskeletal endurance, and gait quality (P &lt; 0.05) were found at the completion of the 8-week intervention.

Conclusion
Preliminary MDORP results are promising in its ability to improve basic and high-level mobility, lower limb strength, and gait quality in a group of SMs and Veterans with LLA. In addition, “booster” prosthetic training may be justified in an effort helps maintain an active lifestyle, promotes prosthetic use, and mitigates secondary health effects.