bluuebunny/crossref_metadata_2025_split huggingface dataset:
Combined LA-ICP-MS/LIBS: powerful analytical tools for the investigation of polymer alteration after treatment under corrosive conditions
Authors: Brunnbauer Lukas , Mayr Maximilian , Larisegger Silvia , Nelhiebel Michael , Pagnin Laura , Wiesinger Rita , Schreiner Manfred , Limbeck Andreas 
Published: July 2020

AbstractPolymers are used in a variety of different areas, including applications in food packaging, automotive and the semiconductor industry. Information about degradation of these materials during application, but also uptake of pollutants from the surrounding environment is therefore of great interest. Conventional techniques used for polymer characterization such as FT-IR or Raman spectroscopy, but also thermo-analytical techniques offer insights into degradation processes but lack the possibility to detect uptake of inorganic species. Moreover, these techniques do not allow the measurement of depth profiles, thus information about degradation or pollutant uptake with sample depth is not accessible. In this work, we propose LA-ICP-MS and LIBS as powerful analytical tools for polymer characterization, overcoming the limitations of conventional analytical techniques used for polymer analysis. Applicability of the developed procedures is demonstrated by the analysis of artificially weathered polyimides and modern art materials, indicating that the degradation of the polymer but also the uptake of corrosive gases is not limited to the sample surface. Finally, a tandem LA-ICP-MS/LIBS approach is employed, which combines the advantages of both laser-based procedures, enabling the simultaneous analysis of polymer degradation and cadmium uptake of polystyrene after exposure to UV radiation and treatment with artificial sea water.
Borrelia burgdorferi spatiotemporal regulation of transcriptional regulator bosR and decorin binding protein during murine infection
Authors: Saputra Elizabeth P. , Trzeciakowski Jerome P. , Hyde Jenny A. 
Published: July 2020

AbstractLyme disease, caused by Borrelia burgdorferi, is an inflammatory multistage infection, consisting of localized, disseminated, and persistent disease stages, impacting several organ systems through poorly defined gene regulation mechanisms. The purpose of this study is to further characterize the spatiotemporal transcriptional regulation of B. burgdorferi during mammalian infection of borrelial oxidative stress regulator (bosR) and decorin binding protein (dbpBA) by utilizing bioluminescent B. burgdorferi reporter strains and in vivo imaging. Fluctuating borrelial load was also monitored and used for normalization to evaluate expression levels. bosR transcription is driven by two promoters, Pbb0648 and PbosR, and we focused on the native promoter. bosR expression is low relative to the robustly expressed dbpBA throughout infection. In distal tissues, bosR was the highest in the heart during in the first week whereas dbpBA was readily detectable at all time points with each tissue displaying a distinct expression pattern. This data suggests bosR may have a role in heart colonization and the induction of dbpBA indicates a RpoS independent transcriptional regulation occurring in the mammalian cycle of pathogenesis. These finding demonstrate that B. burgdorferi engages unknown genetic mechanisms to uniquely respond to mammalian tissue environments and/or changing host response over time.
SIRT7 deficiency suppresses inflammation, induces EndoMT, and increases vascular permeability in primary pulmonary endothelial cells
Authors: Wyman Anne E. , Nguyen Trang T. T. , Karki Pratap , Tulapurkar Mohan E. , Zhang Chen-Ou , Kim Junghyun , Feng Theresa G. , Dabo Abdoulaye J. , Todd Nevins W. , Luzina Irina G. , Geraghty Patrick , Foronjy Robert F. , Hasday Jeffrey D. , Birukova Anna A. , Atamas Sergei P. , Birukov Konstantin G. 
Published: July 2020

AbstractAcute lung injury (ALI), a common condition in critically ill patients, has limited treatments and high mortality. Aging is a risk factor for ALI. Sirtuins (SIRTs), central regulators of the aging process, decrease during normal aging and in aging-related diseases. We recently showed decreased SIRT7 expression in lung tissues and fibroblasts from patients with pulmonary fibrosis compared to controls. To gain insight into aging-related mechanisms in ALI, we investigated the effects of SIRT7 depletion on lipopolysaccharide (LPS)-induced inflammatory responses and endothelial barrier permeability in human primary pulmonary endothelial cells. Silencing SIRT7 in pulmonary artery or microvascular endothelial cells attenuated LPS-induced increases in ICAM1, VCAM1, IL8, and IL6 and induced endomesenchymal transition (EndoMT) with decreases in VE-Cadherin and PECAM1 and increases in collagen, alpha-smooth muscle actin, TGFβ receptor 1, and the transcription factor Snail. Loss of endothelial adhesion molecules was accompanied by increased F-actin stress fibers and increased endothelial barrier permeability. Together, these results show that an aging phenotype induced by SIRT7 deficiency promotes EndoMT with impaired inflammatory responses and dysfunction of the lung vascular barrier.
Estimating illegal fishing from enforcement officers
Authors: Donlan C. Josh , Wilcox Chris , Luque Gloria M. , Gelcich Stefan 
Published: July 2020

AbstractWhile illegal, unreported, and unregulated (IUU) fishing is a premier issue facing ocean sustainability, characterizing it is challenging due to its clandestine nature. Current approaches can be resource intensive and sometimes controversial. Using Chile as an example, we present a structured process leveraging existing capacity, fisheries officers, that provides a monitoring tool to produce transparent and stand-alone estimates on the level, structure, and characteristics of illegal fishing. We provide a national illegal fishing baseline for Chile, estimating illegal activity for 20 fisheries, representing ~ 70% of annual national landings. For four fisheries, we also estimate the relative importance of illegal activities across sectors, stakeholders, and infrastructure. While providing new information, our results also confirm previous evidence on the general patterns of illegality. Our approach provides an opportunity for government agencies to formalize their institutional knowledge, while accounting for potential biases and reducing fragmentation of knowledge that can prevent effective enforcement. Estimating illegal activity directly from fisheries enforcement officers is complementary to existing approaches, providing a cost-effective, rapid, and rigorous method to measure, monitor, and inform solutions to reduce IUU fishing.
Genetic analysis reveals four interacting loci underlying awn trait diversity in barley (Hordeum vulgare)
Authors: Huang Biguang , Huang Daiqing , Hong Zonglie , Owie Swithin Omosuwa , Wu Weiren 
Published: July 2020

AbstractBarley (Hordeum vulgare) awns contribute to grain yield, but the genetic basis of awn development remains largely unclear. Five barley lines differing in awn traits and row types were used to create four F2 populations. Genetic analyses revealed that four pairs of genes were involved in awn development: A/a (awnless/awned), B/b (awnless/awned), H/h (hooded/straight), and L/l (long/short). Of these four loci, A, H and L functioned on both central rows (CR) and lateral rows (LR) of the barley spikes, while B exhibited effect only on LR. A and B had duplicate effects on LR, and both showed dominant epistasis to loci H and L, whereas H was epistatic to L. Meanwhile, A and B were found to be genetically linked, with a row-type locus V located between them. The genetic distances of A-V and B-V were estimated to be 9.6 and 7.7 cM, respectively. Literature search suggested that A, H and V may correspond to the reported Lks1, Kap1 and Vrs1, respectively, whereas B is a novel gene specifically controlling awn development on LR, designated as Lsa1 for lateral spikelet awnless 1. The only barley homolog of wheat awn inhibitor gene B1, HORVU2Hr1G077570, is a potential candidate of Lsa1.
River flow prediction in data scarce regions: soil moisture integrated satellite rainfall products outperform rain gauge observations in West Africa
Authors: Brocca Luca , Massari Christian , Pellarin Thierry , Filippucci Paolo , Ciabatta Luca , Camici Stefania , Kerr Yann H. , Fernández-Prieto Diego 
Published: July 2020

AbstractSatellite precipitation products have been largely improved in the recent years particularly with the launch of the global precipitation measurement (GPM) core satellite. Moreover, the development of techniques for exploiting the information provided by satellite soil moisture to complement/enhance precipitation products have improved the accuracy of accumulated rainfall estimates over land. Such satellite enhanced precipitation products, available with a short latency (< 1 day), represent an important and new source of information for river flow prediction and water resources management, particularly in developing countries in which ground observations are scarcely available and the access to such data is not always ensured. In this study, three recently developed rainfall products obtained from the integration of GPM rainfall and satellite soil moisture products have been used; namely GPM+SM2RAIN, PRISM-SMOS, and PRISM-SMAP. The prediction of observed daily river discharge at 10 basins located in Europe (4), West Africa (3) and South Africa (3) is carried out. For comparison, we have also considered three rainfall products based on: (1) GPM only, i.e., the Early Run version of the Integrated Multi-Satellite Retrievals for GPM (GPM-ER), (2) rain gauges, i.e., the Global Precipitation Climatology Centre, and (3) the latest European Centre for Medium-Range Weather Forecasts reanalysis, ERA5. Three different conceptual and lumped rainfall-runoff models are employed to obtain robust and reliable results over the 3-year data period 2015–2017. Results indicate that, particularly over scarcely gauged areas (West Africa), the integrated products outperform both ground- and reanalysis-based rainfall estimates. For all basins, the GPM+SM2RAIN product is performing the best among the short latency products with mean Kling–Gupta Efficiency (KGE) equal to 0.87, and significantly better than GPM-ER (mean KGE = 0.77). The integrated products are found to reproduce particularly well the high flows. These results highlight the strong need to disseminate such integrated satellite rainfall products for hydrological (and agricultural) applications in poorly gauged areas such as Africa and South America.
Applicability of care quality indicators for women with low-risk pregnancies planning hospital birth: a retrospective study of medical records
Authors: Ueda Kayo , Sado Toshiyuki , Takahashi Yoshimitsu , Igarashi Toshiko , Nakayama Takeo 
Published: July 2020

AbstractPractices for planned birth among women with low-risk pregnancies vary by birth setting, medical professional, and organizational system. Appropriate monitoring is essential for quality improvement. Although sets of quality indicators have been developed, their applicability has not been tested. To improve the quality of childbirth care for low-risk mothers and infants in Japanese hospitals, we developed 35 quality indicators using existing clinical guidelines and quality indicators. We retrospectively analysed data for 347 women in Japan diagnosed with low-risk pregnancy in the second trimester, admitted between April 2015 and March 2016. We obtained scores for 35 quality indicators and evaluated their applicability, i.e., feasibility, improvement potential, and reliability (intra- and inter-rater reliability: kappa score, positive and negative agreement). The range of adherence to each indicator was 0–95.7%. We identified feasibility concerns for six indicators with over 25% missing data. Two indicators with over 90% adherence showed limited potential for improvement. Three indicators had poor kappa scores for intra-rater reliability, with positive/negative agreement scores 0.94/0.33, 0.33/0.95, and 0.00/0.97, respectively. Two indicators had poor kappa scores for inter-rater reliability, with positive/negative agreement scores 0.25/0.92 and 0.68/0.61, respectively. The findings indicated that these 35 care quality indicators for low-risk pregnant women may be applicable to real-world practice, with some caveats.
High diversity and variability of pipolins among a wide range of pathogenic Escherichia coli strains
Authors: Flament-Simon Saskia-Camille , de Toro María , Chuprikova Liubov , Blanco Miguel , Moreno-González Juan , Salas Margarita , Blanco Jorge , Redrejo-Rodríguez Modesto 
Published: July 2020

AbstractSelf-synthesizing transposons are integrative mobile genetic elements (MGEs) that encode their own B-family DNA polymerase (PolB). Discovered a few years ago, they are proposed as key players in the evolution of several groups of DNA viruses and virus–host interaction machinery. Pipolins are the most recent addition to the group, are integrated in the genomes of bacteria from diverse phyla and also present as circular plasmids in mitochondria. Remarkably, pipolins-encoded PolBs are proficient DNA polymerases endowed with DNA priming capacity, hence the name, primer-independent PolB (piPolB). We have now surveyed the presence of pipolins in a collection of 2,238 human and animal pathogenic Escherichia coli strains and found that, although detected in only 25 positive isolates (1.1%), they are present in E. coli strains from a wide variety of pathotypes, serotypes, phylogenetic groups and sequence types. Overall, the pangenome of strains carrying pipolins is highly diverse, despite the fact that a considerable number of strains belong to only three clonal complexes (CC10, CC23 and CC32). Comparative analysis with a set of 67 additional pipolin-harboring genomes from GenBank database spanning strains from diverse origin, further confirmed these results. The genetic structure of pipolins shows great flexibility and variability, with the piPolB gene and the attachment sites being the only common features. Most pipolins contain one or more recombinases that would be involved in excision/integration of the element in the same conserved tRNA gene. This mobilization mechanism might explain the apparent incompatibility of pipolins with other integrative MGEs such as integrons. In addition, analysis of cophylogeny between pipolins and pipolin-harboring strains showed a lack of congruence between several pipolins and their host strains, in agreement with horizontal transfer between hosts. Overall, these results indicate that pipolins can serve as a vehicle for genetic transfer among circulating E. coli and possibly also among other pathogenic bacteria.
Engineering a disulfide-gated switch in streptavidin enables reversible binding without sacrificing binding affinity
Authors: Marangoni Jesse M. , Wu Sau-Ching , Fogen Dawson , Wong Sui-Lam , Ng Kenneth K. S. 
Published: July 2020

AbstractAlthough high affinity binding between streptavidin and biotin is widely exploited, the accompanying low rate of dissociation prevents its use in many applications where rapid ligand release is also required. To combine extremely tight and reversible binding, we have introduced disulfide bonds into opposite sides of a flexible loop critical for biotin binding, creating streptavidin muteins (M88 and M112) with novel disulfide-switchable binding properties. Crystal structures reveal how each disulfide exerts opposing effects on structure and function. Whereas the disulfide in M112 disrupts the closed conformation to increase koff, the disulfide in M88 stabilizes the closed conformation, decreasing koff 260-fold relative to streptavidin. The simple and efficient reduction of this disulfide increases koff 19,000-fold, thus creating a reversible redox-dependent switch with 70-fold faster dissociation kinetics than streptavidin. The facile control of disulfide formation in M88 will enable the development of many new applications requiring high affinity and reversible binding.
Gymnosperm glandular trichomes: expanded dimensions of the conifer terpenoid defense system
Authors: Celedon Jose M. , Whitehill Justin G. A. , Madilao Lufiani L. , Bohlmann Joerg 
Published: July 2020

AbstractGlandular trichomes (GTs) are defensive structures that produce and accumulate specialized metabolites and protect plants against herbivores, pathogens, and abiotic stress. GTs have been extensively studied in angiosperms for their roles in defense and biosynthesis of high-value metabolites. In contrast, trichomes of gymnosperms have been described in fossilized samples, but have not been studied in living plants. Here, we describe the characterization of GTs on young stems of a hybrid white spruce. Metabolite and histological analysis of spruce GTs support a glandular function with accumulation of a diverse array of mono-, sesqui- and diterpenes including diterpene methylesters. Methylated diterpenes have previously been associated with insect resistance in white spruce. Headspeace analysis of spruce GTs showed a profile of volatiles dominated by monoterpenes and a highly diverse array of sesquiterpenes. Spruce GTs appear early during shoot growth, prior to the development of a lignified bark and prior to accumulation of terpenes in needles. Spruce GTs may provide an early, terpene-based chemical defense system at a developmental stage when young shoots are particularly vulnerable to foliage and shoot feeding insects, and before the resin duct system characteristic of conifers has fully developed.
Identifying critical nodes in temporal networks by network embedding
Authors: Yu En-Yu , Fu Yan , Chen Xiao , Xie Mei , Chen Duan-Bing 
Published: July 2020

AbstractCritical nodes in temporal networks play more significant role than other nodes on the structure and function of networks. The research on identifying critical nodes in temporal networks has attracted much attention since the real-world systems can be illustrated more accurately by temporal networks than static networks. Considering the topological information of networks, the algorithm MLI based on network embedding and machine learning are proposed in this paper. we convert the critical node identification problem in temporal networks into regression problem by the algorithm. The effectiveness of proposed methods is evaluated by SIR model and compared with well-known existing metrics such as temporal versions of betweenness, closeness, k-shell, degree deviation and dynamics-sensitive centralities in one synthetic and five real temporal networks. Experimental results show that the proposed method outperform these well-known methods in identifying critical nodes under spreading dynamic.
Synthesis of thiophene-fused heptalenes by cycloaddition of azulenothiophenes with dimethyl acetylenedicarboxylate
Authors: Shoji Taku , Miura Kota , Ariga Yukino , Yamazaki Akari , Ito Shunji , Yasunami Masafumi 
Published: July 2020

AbstractHeptalene has a fused structure of two cycloheptatrienes which is one of the non-aromatic bicyclic molecules with a 12π-electronic structure. We report herein the synthesis of thiophene-fused heptalene derivatives from the corresponding azulenothiophenes via cycloaddition reaction with dimethyl acetylenedicarboxylate. Their structure was clarified by single-crystal X-ray structural analysis. The electronic properties of the thiophene-fused heptalenes obtained by this study were characterized by UV/Vis and fluorescence spectroscopy measurements. The electrochemical features of these derivatives were also examined by voltammetry and spectroelectrochemical experiments.
Pulp regeneration with hemostatic matrices as a scaffold in an immature tooth minipig model
Authors: Jang Ji-Hyun , Moon Joung-Ho , Kim Sahng Gyoon , Kim Sun-Young 
Published: July 2020

AbstractControl of blood clotting in root canal systems is one of the most critical and difficult concerns for regenerative endodontics therapy (RET). The purpose of this study was to investigate the effects of using gelatin- and fibrin-based hemostatic hydrogels as a scaffold on pulp regeneration in a minipig model. Cell viability of human dental pulp stem cells cultured three-dimensionally in gelatin-based and fibrin-based scaffolds was evaluated by MTT and live/dead assay. RET was performed on 24 immature premolars with an autologous blood clot (PC), gelatin-based and fibrin-based hemostatic matrices (GM and FM), or without the insertion of a scaffold (NC). The follow-up period was 12 weeks. Radiographic and histologic assessments for pulp regeneration were performed. Gelatin-based scaffolds exhibited significantly higher cell viability than fibrin-based scaffolds after 15 days (P < 0.05). The PC and GM groups showed favorable root development without inflammation and newly mineralized tissue deposited in the root canal system, while FM group presented inflammatory changes with the continuation of root development. The NC group exhibited internal root resorption with periapical lesions. The application of GM in RET led to favorable clinical outcomes of root development without inflammatory changes compared to conventional RET. Our results suggest that GM may serve as a viable regenerative scaffold for pulp regeneration.
High-temperature optical properties of indium tin oxide thin-films
Authors: Kim Jiwoong , Shrestha Sujan , Souri Maryam , Connell John G. , Park Sungkyun , Seo Ambrose 
Published: July 2020

AbstractIndium tin oxide (ITO) is one of the most widely used transparent conductors in optoelectronic device applications. We investigated the optical properties of ITO thin films at high temperatures up to 800 °C using spectroscopic ellipsometry. As temperature increases, amorphous ITO thin films undergo a phase transition at ~ 200 °C and develop polycrystalline phases with increased optical gap energies. The optical gap energies of both polycrystalline and epitaxial ITO thin films decrease with increasing temperature due to electron–phonon interactions. Depending on the background oxygen partial pressure, however, we observed that the optical gap energies exhibit reversible changes, implying that the oxidation and reduction processes occur vigorously due to the low oxidation and reduction potential energies of the ITO thin films at high temperatures. This result suggests that the electronic structure of ITO thin films strongly depends on temperature and oxygen partial pressure while they remain optically transparent, i.e., optical gap energies > 3.6 eV.
Relevance of temporal cores for epidemic spread in temporal networks
Authors: Ciaperoni Martino , Galimberti Edoardo , Bonchi Francesco , Cattuto Ciro , Gullo Francesco , Barrat Alain 
Published: July 2020

AbstractTemporal networks are widely used to represent a vast diversity of systems, including in particular social interactions, and the spreading processes unfolding on top of them. The identification of structures playing important roles in such processes remains largely an open question, despite recent progresses in the case of static networks. Here, we consider as candidate structures the recently introduced concept of span-cores: the span-cores decompose a temporal network into subgraphs of controlled duration and increasing connectivity, generalizing the core-decomposition of static graphs. To assess the relevance of such structures, we explore the effectiveness of strategies aimed either at containing or maximizing the impact of a spread, based respectively on removing span-cores of high cohesiveness or duration to decrease the epidemic risk, or on seeding the process from such structures. The effectiveness of such strategies is assessed in a variety of empirical data sets and compared to baselines that use only static information on the centrality of nodes and static concepts of coreness, as well as to a baseline based on a temporal centrality measure. Our results show that the most stable and cohesive temporal cores play indeed an important role in epidemic processes on temporal networks, and that their nodes are likely to include influential spreaders.