bluuebunny/crossref_metadata_2025_split huggingface dataset:
Psychiatric, Cognitive Functioning and Socio-cultural Views of Menstrual Psychosis in Oman: An Idiographic Approach
Authors: Al-Sibani Nasser , Al-Maqbali Mandhar , Mahadevan Sangeetha , Al-Huseni Salim , Al-Muzeni Muna , Al-Adawi Samir 
Published: April 2020

Abstract
Background : Most documented cases of menstrual psychosis have been from Euro-American populations with reports from cross-cultural populations being only a few.
Objective : To determine whether the cyclical/episodic nature of menstrual psychosis among case series observed at a tertiary care unit in Oman fulfills the diagnosis criteria of the International Classification of Diseases (ICD-10) and diverge into Brockington’s sub-types (World Psychiatry. 2005;4(1):9-17). Related aims were to solicit measures of psychometric functioning of those with menstrual psychosis and narrated idioms of distress .
Results : The spectrum of distress in people with menstrual psychosis does not fit into existing psychiatric nosology. Evaluations revealed that a majority of the participants displayed something akin to morbid phenomenon related to manic and psychotic symptoms or, in the parlance of Omani society, spirit possession. In terms of classification by timing within the menstrual cycle as expounded by Brockington, the present case series in Oman fulfilled the definition of catamenial psychosis and paramenstrual psychosis. With regard to psychometric function, all participants performed adequately on indices of intellectual functioning but appeared to have impairments in neuropsychological functioning, including dimensions of processing speed, episodic memory, and executive functioning. Within the given society, the periodicity of mind alteration has been attributed to spirit possession.
Conclusions : This is one of the first case series of its kind in the country elucidating whether the manifestation of menstrual psychosis among individuals in Oman fulfills the subtypes postulated by Brockington. The present case series suggests that menstrual psychosis is marked with neuropsychological impairments that were previously observed in other phasic manic episodes or brief psychotic disorders.
The puzzling mitochondrial phylogeography of the black soldier fly (Hermetia illucens), the commercially most important insect protein species
Authors: Ståhls Gunilla , Meier Rudolf , Sandrock Christoph , Hauser Martin , Zorić Ljiljana Šašić , Laiho Elina , Aracil Andrea , Doderović Jovana , Badenhorst Rozane , Unadirekkul Phira , Adom Nur Arina Binte Mohd , Wein Leo , Richards Cameron , Tomberlin Jeffery K. , Rojo Santos , Veselić Sanja , Parviainen Tuure 
Published: April 2020

Abstract
Background The black soldier fly (Diptera: Stratiomyidae, Hermetia illucens ) is renowned for bioconversion ability of organic waste, and is the worldwide most widely used source of insect protein. Despite varying extensively in morphology, it is widely assumed that all black soldier flies belong to the same species, Hermetia illucens . We here use genetic data of 424 rearing culture and wild caught fly samples from 38 countries and six biogeographic regions to test this assumption based on data for three genes (mitochondrial COI, nuclear ITS2 & 28S rDNA). Results Our study reveals a surprisingly high level of intraspecific genetic diversity for the mitochondrial COI gene (divergences up to 4.9%). This level of variability is often associated with the presence of multiple species, but tested nuclear ITS2 and 28S rDNA markers are invariant and fly strain hybridization experiments under laboratory conditions revealed reproductive compatibility. COI haplotype diversity is not only very high in all biogeographic regions (54 distinct haplotypes in total), but also in breeding facilities and research centers from six continents (10 haplotypes: divergences up to 4.3%). The high genetic diversity in fly-breeding facilities is mostly likely explained by many independent acquisitions of cultures via sharing and/or establishing new colonies from wild-caught flies. However, explaining the observed high diversity in most biogeographic regions is difficult. The origin of the species is considered to be New World (32 distinct haplotypes) and one would expect severely reduced genetic diversity in the putatively non-native populations in the remaining biogeographic regions. But distinct, private haplotypes are also known from the Australasian (N=2), Oriental (N=4), and the Palearctic (N=5) populations. We review museum specimen records and conclude that the evidence for introductions is strong for the Western Palearctic and the Afrotropical regions which lack distinct, private haplotypes. Conclusions Based on the results of this paper, we urge the black soldier fly community to apply molecular characterization (genotyping) of the fly strains used in artificial fly-breeding and to share this data in research publications and when sharing cultures. In addition, fast-evolving nuclear markers should be used to reconstruct the recent invasion history of the species.
Integrated analysis of differentially expressed long noncoding RNAs and mRNAs associated with high-fat diet-induced hepatic insulin resistance in mice
Authors: Zhou Zengyuan , Zhao Xue , Chen Liang , Li Yuzheng , Chen Zhao , Wang Yuanyuan , Zhou Zihao , Chu Xia 
Published: April 2020

Abstract
Background: Hepatic insulin resistance (IR) is an early pathological characteristic of many metabolic diseases, such as type 2 diabetes. Long noncoding RNAs (lncRNAs) have been identified as mediators of IR and related diseases. However, the roles of lncRNAs in hepatic IR remain largely unknown. Method: High-throughput sequencing was performed on ten liver tissue samples from five normal diet (ND)-fed mice and five high-fat diet (HFD)-induced hepatic IR mice, respectively. lncRNAs and mRNAs that were differentially expressed (DE) between the two groups were identified by bioinformatic analyses. Seven DE lncRNAs were validated by quantitative real-time PCR (q-PCR). The potential functions of the DE lncRNAs were predicted by Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses of target genes. In addition, integrated analysis was performed for the DE lncRNAs and mRNAs to predict their interaction relationships.Results: A total of 232 DE lncRNAs were identified in the HFD-induced hepatic IR mice compared with the ND-fed mice. These DE lncRNAs included 108 upregulated and 124 downregulated lncRNAs, and 7 of the DE lncRNAs were validated by q-PCR. In addition, 291 DE mRNAs including 166 upregulated and 125 downregulated mRNAs were identified in the HFD group. Furthermore, target genes of the DE lncRNAs were predicted, and functional enrichment results showed that the enriched genes were involved in IR- and glycolipid metabolism-related processes. Additionally, the coexpression network was also constructed to further reflect the potential functions of the DE lncRNAs.Conclusion: The study describes the expression profiles of lncRNAs and mRNAs and the functional networks involved in HFD-induced hepatic IR. These findings may provide a new perspective for the study of lncRNAs in hepatic IR- and glycolipid metabolism-related diseases.
Rapid Detection of Babesia motasi Responsible for Human Babesiosis by Cross-priming Amplification Combined with a Vertical Flow
Authors: Wang Jinming , Gao Shandian , Zhang Shangdi , He Xin , Liu Junlong , New Aihong Liu , Li Youquan , Liu Guangyuan , Luo Jianxun , Guan Guiquan , Yin Hong 
Published: April 2020

Abstract
Background Babesia motasi is known as an etiological agent of human and ovine babesiosis. Diagnosis of babesiosis is traditionally performed by microscopy, examining Giemsa-stained thin peripheral blood smears. Rapid detection and accurate identification of species are desirable for clinical care and epidemiological studies. Methods An easy to operate molecular method, which requires less capital equipment and incorporates cross priming amplification combined with a vertical flow (CPA-VF) visualization strip for rapid detection and identification of B. motasi. Results The CPA-VF targets the 18S rRNA gene and has a detection limit of 50 fg per reaction; no cross reaction was observed with other piroplasms infective to sheep or Babesia infective to humans. CPA-VF and real-time (RT)-PCR had sensitivities of 95.2% (95% confidence interval [CI], 78.1-99.4%) and 90.5% (72-97.6%) and specificities of 95.8 (80.5-99.5%) and 97.9 (83.5-99.9%), respectively, versus microscopy and nested (n) PCR combined with gene sequencing. The clinical performance of the CPA-VF assay was evaluated with field blood samples from sheep (n = 240) in Jintai county, Gansu Province, and clinical specimens (n = 492) obtained from patients bitten by ticks. Conclusions Our results indicate that the CPA-VF is a rapid, accurate, nearly instrument-free molecular diagnostic approach for identification of B. motasi. Therefore, it could be an alternative technique for epidemiological investigations and diagnoses of ovine and/or human babesiosis caused by B. motasi, especially in resource-limited regions.
Inapropriate Use of Antibiotics Effective Against Gram Positive Microorganisms Despite Restrictive Antibiotic Policies in ICUs: A Prospective Observational Study
Authors: Özger Hasan Selçuk , Fakıoğlu Dolunay Merve , Erbay Kübra , ALBAYRAK ASLINUR , Hızel Kenan 
Published: April 2020

Abstract
Background: Gram-positive spectrum antibiotics such as vancomycin, teicoplanin, daptomycin, and linezolid are frequently used in empirical treatment combinations in critically ill patients. Such inappropriate and unnecessary widespread use, leads to sub-optimal utilisation. However they are covered by the antibiotics restriction programme. This prospective observational study, evaluates gram-positive anti-bacterial utilisations in intensive care units (ICUs) with various evaluation criteria, to determine the frequency of inappropriate usage and the intervention targets required to ensure optimum use.
Methods: This clinical study was conducted prospectively between 01.10.2018 and 01.10.2019 in the medical and surgical ICUs of Gazi University Faculty of Medicine Hospital, Turkey. The total bed capacity was 55. Patients older than 18 years and who were prescribed gram-positive spectrum antibiotics (vancomycin, teicoplanin, linezolid, and daptomycin) were included. Patients under this age or immunosuppressed patients (neutropenic,- HIV-infected patients with hematologic or solid organ malignancies) were not included in the study. During the study period, 200 treatments were evaluated in 169 patients. The demographic and clinical features of the patients were recorded. Besides observations by the clinical staff, the treatments were recorded and evaluated by two infectious diseases specialists and two clinical pharmacists at 24-h intervals from the first day to the last day of treatment.
SPSS software for Windows, (version 17, IBM, Armonk, NY) was used to analyse the data. Categorical variables were presented as number and percentage, and non-categorical variables were presented as mean ± standard deviation.
Results: It was found that inappropriate gram-positive antibiotic use in ICUs was as high as 83% in terms of non-compliance with the selected quality parameters. Multivariate analysis was performed to evaluate the factors associated with inappropriate antibiotic use, increased creatinine levels were found to increase the risk of such use.
Conclusions: In spite of the restricted antibiotics programme, inappropriate antibiotic use in ICUs is quite common. Thus, it is necessary to establish local guidelines in collaboration with different disciplines for the determination and follow-up of de-escalation of such use and optimal treatment doses.
Keywords: Antibiotic stewardship, rational antibiotic use, antibiotic resistance, gram positive microorganisms, inappropriate antibiotic use
Diversity of MSDIN family members in amanitin-producing mushrooms and the phylogeny of the MSDIN and prolyl oligopeptidase genes
Authors: He Zhengmi , Long Pan , Fang Fang , Li Sainan , Zhang Ping , Chen Zuohong 
Published: April 2020

Abstract
Background: Amanitin-producing mushrooms, mainly distributed in the genera Amanita , Galerina and Lepiota , possess MSDIN gene family for the biosynthesis of many cyclopeptides catalysed by prolyl oligopeptidase (POP). Recently, transcriptome sequencing has proven to be an efficient way to mine MSDIN and POP genes in these lethal mushrooms. Thus far, only A . palloides and A. bisporigera from North America and A . exitialis and A. rimosa from Asia have been studied based on transcriptome analysis. However, the MSDIN and POP genes of many amanitin-producing mushrooms in China remain unstudied; hence, the transcriptomes of these speices deserve to be analysed.
Results: In this study, the MSDIN and POP genes from ten Amanita species, two Galerina species and Lepiota venenata were studied and the phylogenetic relationships of their MSDIN and POP genes were analysed. Through transcriptome sequencing and PCR cloning, 19 POP genes and 151 MSDIN gened to encode 98 non-duplicated cyclopeptides, including α- amanitin, β-amanitin, phallacidin, phalloidin and 94 unknown peptides, were found in these species. Phylogenetic analysis showed that (1) MSDIN genes generally clustered depending on the taxonomy of the genus, while Amanita MSDIN genes clustered depending on the chemical substance; and (2) the POPA genes of Amanita , Galerina and Lepiota clustered and were separated into three different groups, but the POPB genes of the three distinct genera were clustered in a highly supported monophyletic group.
Conclusions: These results indicate that lethal Amanita species have the genetic capacity to produce numerous cyclopeptides, most of which are unknown, while lethal Galerina and Lepiota species seem to only have the genetic capacity to produce α-amanitin. Additionally, the POPB phylogeny of Amanita , Galerina and Lepiota conflicts with the taxonomic status of the three genera, suggesting that underlying horizontal gene transfer has occurred among these three genera.
Hemodiafiltration with ultrafiltrate regeneration reduces free light chains without albumin loss in multiple myeloma patients.
Authors: de Mier M. Victoria Pendon-Ruiz , Ojeda Raquel , Álvarez-Lara M. Antonia , Navas Ana , Alonso Corona , Caballero-Villarraso Javier , Aljama Pedro , Álvarez Miguel A. , Soriano Sagrario , Rodríguez Mariano , Martín-Malo Alejandro 
Published: April 2020

Abstract
Background Acute kidney injury (AKI) occurs in 12-20% of multiple myeloma (MM) patients. Several studies have shown a reduction of free light chains (FLC) using hemodialysis with High-Cut-Off membranes. However, this technique entails albumin loss. Hemodiafiltration with ultrafiltrate regeneration is a technique that includes a process of adsorption. The aim of this study was to evaluate the effectiveness of hemodiafiltration with ultrafiltrate regeneration in reducing FLC levels without causing albumin loss. Methods This is an observational study (2012 to 2018) including eleven patients with MM (6 kappa, 5 lambda) and AKI. All patients were treated with chemotherapy and hemodiafiltration with ultrafiltrate regeneration. Blood Samples (pre and post-dialysis) and ultrafiltrate were collected pre and post-resin at 5 minutes after initiation of the session and 5 minutes before the end of the procedure. Results The serum levels of kappa and lambda were reduced by a 53±14% and 33±22% respectively. Serum albumin concentration remained unchanged after the procedure. In the ultrafiltrate, the mean FLC reduction ratio shortly after initiation of the dialysis procedure was: 99.2% and 97.06% for kappa and lambda respectively, and only 0.7% for albumin; and at the end of the session the percent reduction was: 63.7% and 33.62% for kappa and lambda respectively, and 0.015% for albumin. Patients clinical outcome was: 36.4% recovered renal function, 18.2% died during the first year and 45.45% required maintenance dialysis. Conclusions Hemodiafiltration with ultrafiltrate regeneration reduces FLC levels without producing a significant loss of albumin; and, FLC removal is maintained throughout the session. Therefore, hemodiafiltration with ultrafiltrate regeneration may be considered an effective adjunctive therapy in patients with MM.
Clinicopathologic Features of Infection-Related Glomerulonephritis with IgA Deposits: a French Nationwide Study
Authors: Miquelestorena-Standley Elodie , Jaulerry Charlotte , Machet Marie-Christine , Rabot Nolwenn , Barbet Christelle , Hummel Aurélie , Karras Alexandre , Garrouste Cyril , Crepin Thomas , Ducloux Didier , Cousin Maud , Albert Catherine , Rivalan Joseph , Gall Emilie Cornec Le , Pourreau François , Deltombe Clément , Nochy Dominique , Szlavik Nora , Felix Sophie , Croué Anne , Buob David , Rioux-Leclerc Nathalie , Doucet Laurent , Goujon Jean-Michel , Renaudin Karine , New Emmanuelle Blanchard , New Sébastien Eymieux , Rabant Marion , Halimi Jean-Michel 
Published: April 2020

Abstract
Background: Infection-related glomerulonephritis with IgA deposits (IRGN-IgA) is a rare disease but it is increasingly reported in the literature. Data regarding epidemiology and outcome are lacking, especially in Europe. We aimed to assess the clinical, pathologic and outcome data of IRGN-IgA.
Methods: Clinical and outcome data from patients from 11 French centers over the 2007-2017 period were collected retrospectively. We reviewed pathologic patterns and immunofluorescence of renal biopsies and evaluated C4d expression in IRGN-IgA. We analyzed the correlation between histological presentation and outcome.
Results: Twenty-seven patients (23 men, mean age: 62±15 years) were included. Twenty-one (78%) had Staphylococcus aureus infection and twelve (44%) were diabetic. At the time of biopsy, 95.2% had haematuria, 48.1% had a serum creatinine level of >4 mg/dL, and 16% had hypocomplementemia. The most common pathologic presentation included mesangial (88.9%) and endocapillary proliferative glomerulonephritis (88.9%) with interstitial fibrosis and tubular atrophy (IF/TA) (85.1%). Diffuse and global glomerular C4d expression was found in 17.8%, mostly in biopsies with acute or subacute patterns, and was associated with a short delay between infection and renal biopsy compared to segmental and focal staining. After median follow-up of 13.2 months, 23.1% died, 46.2% had persistent renal dysfunction and 15.4% reached end-stage renal disease. Renal outcome was correlated to IF/TA severity.
Conclusions: Infection-related glomerulonephritis with IgA deposits is usually associated with Staphylococcus infections and mainly affects adult men. This entity has a poor prognosis which is correlated to interstitial fibrosis and tubular atrophy severity.
High-temperature requirement factor A1 rs11200638 polymorphism and age-related macular degenerative from a comprehensive analysis about 15316 subjects
Authors: Liu Ying , Jin Huipeng , Wei Dong , Li Wenxiu 
Published: April 2020

Abstract
Background The high-temperature requirement factor A1 (HTRA1) gene at the 10q26 locus has been associated with age-related macular degenerative (AMD) risk, with the significantly associated polymorphism being (rs11200638, -625G/A), however, above association is not consistent. We investigated an updated meta-analysis to evaluate the association between rs11200638 polymorphism and AMD risk thoroughly addressing this issue. Methods An identification was covered with the Pubmed and Chinese Wanfang databases through 27th Jan, 2020. Odds ratios (OR) and 95% confidence intervals (CI) were used to assess the strength of associations. After a thorough and meticulous search, 35 different articles (33 case-control studies with HWE, 22 case-control studies about wet/dry AMD) were retrieved. Results Individuals carrying A-allele or AA genotype may have an increased risk to be AMD disease. For example, there has a significantly increased relationship between rs11200638 polymorphism and AMD both for Asians (OR: 2.50, 95%CI: 2.22-2.80 for A-allele vs. G-allele) and Caucasians (OR: 2.11, 95%CI: 1.43-3.13 for A-allele vs. G-allele). Moreover, a similar trend in the source of control subgroup was detected. To classify the type of AMD, increased association was also observed in both wet (OR: 3.40, 95%CI: 2.90-3.99 for dominant model) and dry (OR: 2.08, 95%CI: 1.24-3.48 for dominant model) AMD. Finally, based on the different genotyping methods, increased relationships were identified by sequencing, TaqMan, PCR-RFLP. Conclusions Our present meta-analysis suggests that the HTRA1 rs11200638 polymorphism are potentially associated with the risk of AMD development, especially about individuals carrying A-allele or AA genotype, who may be as identified targets to detect and intervene in advance. Further studies using larger sample sizes and including information about gene-environment interactions should be conducted to elucidate.
Prognostic value of neuron specific enolase in patients with advanced and metastatic non-neuroendocrine non-small cell lung cancer
Authors: Yan Peng , Han Yi-ming , Liu Jie , Zuo Tai-yang , Wang Xin-yu , Liu Chuan-yong 
Published: April 2020

Abstract
Background: Increased serum NSE levels were found in a substantial proportion (30%-69%) of patients with NSCLC, but little is known about the clinical properties of neuron specific enolase (NSE).
Objective: We aimed to access the level of serum NSE to predict the prognosis and treatment response in advanced or metastatic non-neuroendocrine non-small cell lung cancer.
Methods: We retrospectively analyzed 363 patients with advanced and metastatic NSCLC (without neuroendocrine immunohistochemistry stain and brain metastasis) between January 2011 and October 2016. The serum NSE level was measured before initiation of treatment.
Results: According to the X-tile program, the optimum cut-off value for NSE level was 26.1 ng/ml. Patients with elevated NSE level (≥26.1 ng/ml) showed significantly shorter progression-free survival (PFS) (median PFS, 4.31 months vs 9.93 months; P < 0.001) and significantly shorter overall survival (OS) (median OS, 8.13 months vs 17.11 months; P < 0.001).
In subgroup analysis, patients with elevated NSE level (≥26.1 ng/ml) showed significantly shorter PFS than those with low NSE level (<26.1ng/ml) in EGFR mutations group and EGFR-wide type group (6.58 months vs 16.02 months, P = 0.0016; 3.42 months vs 7.46 months, P < 0.001, respectively). In terms of OS, the patients with elevated NSE was also significantly shorter than those with low NSE in EGFR mutations group and EGFR-wide type group (9.51 months vs 27.73 months, P < 0.001; 7.76 months vs 13.55 months, P < 0.001, respectively).
Univariate and multivariate analysis demonstrated that elevated NSE level was independent prognostic factors for short PFS (hazard ratio [HR] = 2.40 (1.71-3.38), P < 0.001; HR = 1.81 (1.28-2.56), P = 0.001, respectively) and OS (HR, 2.40 (1.71-3.37), P < 0.001; HR, 1.76 (1.24-2.50), P = 0.002, respectively).
Conclusion: The survival of NSCLC with elevated serum NSE level was shorter than that of NSCLC with low NSE level. Serum NSE level is a predictor of treatment and independent prognostic factor.