bluuebunny/crossref_metadata_2025_split huggingface dataset:
Enchondroma of the spine: a report of eleven cases
Authors: Liu Dehuai , Li Xiangbo , Luo Kai , Lin Chengsen , Chen Xiangxiang , Liu Yun , Tang Haijun 
Published: April 2020

Abstract
Purpose To explore clinical characteristics, surgical strategies, and prognosis of spinal enchondroma. Methods We retrospectively analyzed eleven cases with primary benign spinal enchondroma who were treated in our institution between January 2008 and December 2016. Results Of the 11 patients included in our study, the average age was 43 years(range from 5–66 years). There were 7 men and 4 women. The lesions were mainly located in the lumbar and thoracic column with 5 cases and 4 cases respectively, and following by the cervical and sacral vertebrae with both 1 cases. Total tumor resection was used in 10 cases and curettage in one case. The average follow-up was 3.3 years(range from 2 to 6 years). Most of the cases had achieved satisfied therapeutic outcome with the symptoms disappeared. However, Case 9 developed into local recurrence and a second surgery was performed. Although the lesion have not recured any more after the second operation during the follow-up, the lower extremity paralysis had no improvement. Case 2 was sent to our hospital for the recurrence of the sacral tumor which was very closed to the common iliac artery.When the second operation was conducted, the artery was ruptured and the patient died from hemorrhagic shock. Conclusion To establish the diagnosis of spinal enchondroma is not always a simple job for the absence of specific clinical characteristics. The optimal treatment is total resection. Although spinal enchondroma is benign, a few cases can develop into local recurrence. Sacral enchondroma should be more vigilant for its fatal complications.
Transcortical approach for the treatment of large intraventricular central neurocytoma
Authors: Zhang Chaocai , Yu Lanbing , Hao Shuyu , Fang Yehong , Jia Heyuan , Wang Junling , Wang Xingchao , Ji Nan , Cen Bo , Zhao Jiannong , Gao Zhixian 
Published: April 2020

Abstract
Background Central neurocytoma is a rare intracranial tumor. Due to the limited number of reported cases in the past, there is still controversy about the treatment strategy for central neurocytoma, especially large central neurocytomas occupying bilateral ventricles. This paper will discuss our clinical experience in the treatment of large central neurocytoma. Methods A retrospective analysis of 29 patients with intraventricular central neurocytoma diagnosed and treated by the same surgeon between April 2012 and September 2019 at the Neurosurgery Departments of Beijing Tiantan Hospital and Hainan General Hospital was performed. The clinical characteristics, treatment plan and prognosis of these patients were discussed. Results Among the 29 patients, the average age of onset was 31.93 (17-61) years, and the average maximum tumor diameter was 54.17 ± 15.62 mm. Among them, 23 patients (79.3%) had an average maximum diameter greater than 5 cm. Twenty-seven patients (93.1%) underwent transcortical tumor resection. Twenty-seven patients (93.1%) achieved gross total tumor resection. Five patients (17.2%) received radiotherapy after the first operation, and 3 patients (10.3%) had tumor recurrence. None of the patients died. Conclusion Operation is the basis of the treatment of central neurocytoma. It is possible for patients to be cured by total resection of the tumor and protection of normal ventricular structure. Transcortical fistulotomy is a reliable approach for the removal of large central neurocytomas in the ventricle. Patients should be closely observed post-operation, and MRI of the head should be rechecked regularly. If tumor progression occurs, then radiotherapy should be considered.
Components of antenatal care received by women in fishing communities on Lake Victoria, Uganda; a cross sectional survey
Authors: Ssetaala Ali , Nabawanuka Joan , Matovu Gideon , Nakiragga Nusula , Namugga Judith , Nalubega Phiona , Kaluuma Henry Lutalo , Chinyenze Kundai , Perehudoff Katrina , Michielsen Kristien , Bagaya Bernard , Price Matt , Kiwanuka Noah , Degomme Olivier 
Published: April 2020

Abstract
Background: Uganda has one of the highest maternal deaths at a ratio of 336 per 100,000 live births. As Uganda strives to achieve sustainable development goals, components of antenatal care are key to reduction of maternal mortality. We explored women’s receipt of seven of the Uganda guidelines components of antenatal care, and associated factors in hard to reach Lake Victoria island fishing communities of Kalangala district.Methods: A cross sectional survey among 486 consenting women aged 15-49 years, who were pregnant or had a birth or abortion in the past 6 months was conducted in 6 island fishing communities of Kalangala district, Uganda, during January-May 2018. Interviewer administered questionnaires, in Open Data Kit software were used to collect data on socio-demographics and receipt of seven of the Uganda guidelines components of antenatal care. Regression modeling was used to determine factors associated with receipt of all seven components. Analyses were done using STATA version 15.1.Results: Women’s median (range) age was 26 (15-45) years, majority were married [87.0%, (423/486)], their main occupation was housewives (stay home mums) [45.1%, (219/486)] and never studied beyond seven years of education [69.1%, (336/486)]. Over three quarters of women who attended the nationally recommended four focused ANC visits did not receive all seven components of care at least once during the visits [76.4%, (107/140), P<0.05]. Women who reported being attended to by a doctor were thrice as likely to have received all seven components as those seen by a midwife/nurse (aOR=3.2; 95% CI:1.1-9.1).Conclusions: There is need to improve components of antenatal care received by women in these communities. Implementation programs need to evaluate receipt of antenatal care components among attendees in these hard to reach communities.Trial Registration: PACTR201903906459874 (Retrospectively registered).
A retrospective analysis of paraspinal muscle-splitting microscopic-assisted discectomy versus percutaneous endoscopic lumbar discectomy on patients in the treatment of far-lateral lumbar disc herniation
Authors: KONG LEI , Xu Hong Guang 
Published: April 2020

Abstract
Background : Minimally invasive surgery includes percutaneous endoscopic lumbar discectomy and the microscopic tubular technique. This study aimed to compare the two techniques and evaluate the outcomes of the procedures.
Methods : We retrospectively analyzed patients with far-lateral lumbar disc herniation (FLLDH) from June 2015 to October 2018. Twenty-six patients underwent paraspinal muscle-splitting microscopic-assisted discectomy (MD) and 30 patients underwent percutaneous endoscopic lumbar discectomy (PELD) surgery by the same surgical team. Data included the duration of the operation, duration of intraoperative radiation exposure, and average duration of hospitalization. Pre- and postoperative pain scores and neurological functions were recorded using a visual analog scale (VAS) score and Oswestry disability index (ODI).
Results: 56 patients remained in the study over the 12–24 months period. The mean operating time was 65.83 ± 16.64 min in the PELD group, mean duration of radiation exposure was 2.87 ± 1.19 min, and average of hospitalization was 3.43 days. The mean operating time was 44.96 ± 16.87 min in the MD group, duration of radiation exposure was 0.78 ± 0.32 min, and duration of hospitalization was 4.12 days. Except low back pain at 3 months (p >0.05), all patients in both groups showed significant improvement in VAS and ODI scores compared with pre-operation and until final follow-up (p<0.05).
Conclusion: Both techniques are minimally invasive, effective, and safe for treating far-lateral lumbar disc herniation in selected patients. Compared with the PELD technique, the MD procedure offers a wider field of vision during operation, shorter operation time, fewer postoperative complications, and shorter learning curve.
Risk factors for one-year all-cause mortality in elderly multimorbid medical in-patients.
Authors: Wang-Hansen Marte Sofie , Kersten Hege , Wyller Torgeir Bruun 
Published: April 2020

Abstract
Background Due to expansion of longevity, the proportion of elderly people with multimorbidity is increasing. Our aim was to identify risk factors for all-cause mortality in elderly multimorbid medical in-patients one year after acute hospitalization. Information regarding risk factors is important to support targeted care plans after discharge. Methods Prospective cohort study of patients acutely admitted to a medical department in a Norwegian regional hospital. Eligible patients were community-dwelling, received home care services before hospitalization, were aged 75+, and suffered from two or more chronic conditions. Inclusion period was 1. April – 31. October 2012. Candidate variables were number of daily prescribed drugs, Cumulative Illness Rating Scale for Geriatrics score, delirium, body mass index (BMI), handgrip strength, Barthel Activities of Daily Living Index score, and the laboratory analyses haemoglobin (Hgb), sodium (Na) and estimated glomerular filtration rate (eGFR). Results We included 227 patients; mean age 86 years, 59% women, 71% lived alone. During the year after hospitalization, 39% died. In the adjusted cox proportional hazards regression analysis, significant and independent risk factors were BMI (hazard ratio (HR) 0.93, 95% confidence interval (CI) 0.88-0.98 per kg/m 2 ), Hgb (HR 0.87, 95% CI 0.76-0.98 per g/100 mL), Na (HR 0.94, 95% CI 0.90-0.99 per mmol/L) and eGFR < 60 mL/min/1.73 m 2 (HR 1.82, 95% CI 1.07-3.08). Conclusions In this cohort of multimorbid elderly internal medicine patients, low body mass, hyponatremia, impaired renal function and anaemia were identified as independent and significant risk factors for one-year mortality.
Survival outcomes of neoadjuvant versus adjuvant chemotherapy in triple-negative breast cancer: a meta-analysis in 36480 cases.
Authors: Xia Lin-Yu , Hu Qing-Lin , Zhang Jing , Xu Wei-Yun , Li Xiao-Shi 
Published: April 2020

Abstract
Background The survival outcomes of neoadjuvant chemotherapy(NACT) versus adjuvant chemotherapy(ACT) in triple-negative breast cancer (TNBC) remains unclear. Therefore, in this study, a meta-analysis was conducted to analyze current evidence on the survival outcomes of neoadjuvant versus ACT in TNBC. Methods A systematic search of PubMed and Embase databases were done to identify relevant articles investigating the survival outcomes of NACT versus ACT in TNBC. Results A total of nine studies involving 36480 patients met the selection criteria.Of them,10728(29.41%) received NACT, and 25752(70.59%) received ACT.The pathological complete response (pCR) rate is 35% (95% CI = 0.23-0.48). Compared with ACT, the overall survival(OS) of NACT was poor(HR = 1.59; 95%CI = 1.25-2.02; P=0.0001), and there was no significant difference in disease-free survival(DFS) between them(HR = 0.85; 95%CI = 0.54-1.34; P=0.49). NACT with pCR can significantly improve the OS(HR = 0.53; 95%CI = 0.29-0.98; P=0.04) and DFS(HR = 0.52; 95%CI = 0.29-0.94; P=0.03), while the OS(HR = 1.18; 95%CI = 1.09-1.28; P<0.0001) and DFS(HR = 2.36; 95%CI = 1.42-3.89; P=0.0008) of patients with residual disease(RD) following NACT were worse compared with ACT. Conclusion The findings suggest that, for TNBC, Patients receiving NACT with pCR can significantly improve OS and DFS compared with ACT and it turns to be opposite when patients receiving NACT with RD.
Screening and identification of key biomarkers and related transcription factors in ovarian cancer by integrated bioinformatics analysis
Authors: Qin Wenqiong , Yuan Qiang , Liu Yi , Zeng Ying , Ke Dandan , Dai Xiaoyan , Shuai Yu , Hu Jiaqi , Shi Hua 
Published: April 2020

Abstract
Background Ovarian tumors are the most malignant tumors of all gynecological tumors, and although multiple efforts have been made to elucidate the pathogenesis, the molecular mechanisms of ovarian cancer remain unclear. Methods In this study, we used bioinformatics to identify genes involved in the carcinogenesis and progression of ovarian cancer. Three microarray datasets (GSE14407, GSE29450, and GSE54388) were downloaded from Gene Expression Omnibus (GEO) database, and differentially expressed genes (DEGs) were identified. For a more in-depth understanding of the DEGs, functional and pathway enrichment analyses were performed and a protein-protein interaction (PPI) network was constructed. The associated transcriptional factor (TFs) regulation network of the DEGs was also constructed. Kaplan Meier-plotter, Gene Expression Profiling Interactive Analysis (GEPIA), the Human Protein Atlas (HPA) database and the Oncomine database were implemented to validated hub genes. Results A total of 514 DEGs were detected after the analysis of the three gene expression profiles, including 171 upregulated and 343 downregulated genes. Nine hub genes ( CCNB1, CDK1, BUB1, CDC20, CCNA2, BUB1B, AURKA, RRM2, TTK) were obtained from the PPI network. Survival analysis showed that high expression levels of seven hub genes ( CCNB1, BUB1, BUB1B, CCNA2, AURKA, CDK1, and RRM2) were associated with worse overall survival (OS). All of seven hub genes were discovered highly expressed in ovarian cancer samples compared to normal ovary samples in GEPIA. Immunostaining results from the HPA database suggested that the expressions of CCNB1, CCNA2, AURKA, and CDK1 proteins were increased in ovarian cancer tissues, and Oncomine analysis indicated that the expression patterns of BUB1B, CCNA2, AURKA, CCNB1, CDK1, and BUB1 have associated with patient clinicopathological information. From the gene-transcriptional factor network, key transcriptional factors, such as POLR2A, ZBTB11, KLF9, and ELF1, were identified with close interactions with these hub genes. Conclusion We identified six significant DEGs with poor prognosis in ovarian cancer, which could be of potential biomarkers for ovarian cancer patients.
A case report and summary of thrombocytopenia caused by coronavirus disease 2019
Authors: Zhao Yibing , Du Na , Li Wei , Xiang Dongmei , Zhou Guodong , Li Ruixu , Xu Panyang , Xu Jiancheng 
Published: April 2020

Abstract
Background: Coronavirus disease 2019 has become a major problem for the world. We reported a representative case with thrombocytopenia.Case report: A 44-year-old female patient sought medical attention for fatigue and cough of 1 day duration. Thirteen days before disease onset, the patient had returned from Hubei province. Her body temperature on admission was 38.6℃. Blood routine test showed a white blood cell count of 4.0×109/L, lymphocyte count of 1×109/L, platelet count of 118×109/L, and C-reactive protein of 6.0mg/L. Lung computed tomography (CT) scan showed: large patches of ground-glass opacities at the posterior segment of the right upper lung lobe and bilaterally at the basal segment of the lower lung lobes. Her throat swabs tested positive for novel coronavirus nucleic acid. After antiviral and symptomatic treatment were given, on day 5 of hospitalization, her symptoms of coughing and chest pain worsened after coughing, and she also developed hemoptysis. Repeated lung CT scans showed that the lesion area had increased. The lymphocyte count had decreased to 0.55×109/L and the platelet count had decreased to 9×109/L on day 6. After a series of multimodal therapies were carried out, the patient’s platelet and lymphocyte count gradually increased to normal.Conclusion: We reported a COVID-2019 case with significant thrombocytopenia, which was more significantly decreased than in normal viral infections. However, the mechanism of thrombocytopenia is unknown and requires further research.
Insights on the forming process of reciprocal gene loss in the duplicate DPL genes of rice
Authors: Zhang Fu-min , Xu Xun , Ge Song 
Published: April 2020

Abstract
Background
Reciprocal gene loss (RGL) of duplicate genes is an important genetic source of reproductive isolation, but the forming process of RGL remains poorly understood, particularly when concerning a single gene duplication. The RGL of the duplicate DOPPELGANGER1 ( DPL1 ) and DOPPELGANGER2 ( DPL2 ) gene can lead to BDM-type hybrid incompatibility between two rice subspecies. The evolutionary history of the duplicate genes, including their origin and mechanism of duplication as well as their evolutionary divergence after the duplication, is crucial for understanding the RGL process. Thus, we attempted to elucidate the evolutionary history of the duplicate genes for gaining insights into the forming process of RGL.
Results
We reconstructed phylogenetic relationships of DPL copies from all 15 diploid species representing six genome types of rice genus and then found that all the DPL copies from the latest diverged A- and B-genome gather into one monophyletic clade. Southern blot analysis also detected definitely two DPL copies only in A- and B-genome. High conserved collinearity can be observed between the DPL1 segments of A- and B-genome and between the DPL 2 segments of A- and B-genome respectively but not between DPL1 and DPL2 segments. Investigations of transposon elements indicated that DPL duplication is more likely to be produced by DNA transposons. Likelihood-based analyses with branch models showed a relaxation of selective strength on DPL1 lineage but an enhancement of selective constraint on DPL2 lineage after DPL duplication. Sequence analysis also indicated that defective DPL1 can be found in six wild and cultivated species out of the eight species of A-genome but only one defective DPL2 occurs in a cultivated rice subspecies.
Conclusions
DPL duplication of rice originated in the most recent common ancestor of A- and B-genome about 6.76 million years ago and the duplication was possibly caused by DNA transposons. The DPL1 is a redundant copy and has being in the process of pseudogenization, suggesting that artificial selection may play an important role in forming the RGL of DPL genes between two rice subspecies during the domestication.
Keywords: Reciprocal gene loss, Gene duplication, Evolution history, DPL , Rice
Clinical and Safety Outcomes of Conversion Original Tacrolimus to Generic Tacrolimus in Turkish Kidney Transplant Recipients
Authors: Gunay Emrah 
Published: April 2020

Abstract
Background

After the United States, The Food and Drug Administration approved the use of the first generic of the original tacrolimus in 2009, generic tacrolimus was preferred in many countries for cost-reducing reasons. This is the first study on the conversion of original tacrolimus to the generic tacrolimus, reported from Turkey.

Methods

The inclusion criteria of this single-center, retrospective study were 1) being ≥18 years old, 2) passing at least three months after kidney transplantation, 3) conversion from reference tacrolimus (Prograf®) to generic tacrolimus (Adoport®). The primary endpoints were acute rejection, an increase in serum creatinine, decreases in e-GFR, tacrolimus level, tacrolimus concentration/dose ratio. We applied the paired T-test to analyze the pre-conversion and final visit laboratory values.

Results

Thirty-six patients who agreed to use generic tacrolimus evaluated. The mean age was 39.8 years (± 11.6), % 52,7 were female, % 86,1 were living donor transplants. The patients followed up for 12 months (3-41). There was no increase in serum creatinine value, no decreases in e-GFR and tacrolimus concentration/dose ratio. We observed a decrease in tacrolimus level (p=0,037). Decreasing the target value may have caused this result, as there are 9 patients with positive BK-DNA. None of the patients needed a biopsy.

Conclusion

Based on the results of our study, renal outcomes are safe and the drugs could be changed safely. Whether Prograf® or Adoport®, whichever is used, it is important to continue taking the drug at the recommended dose and time. The physician should be careful in dose adjustment after conversion, especially for those who are in the first year of transplantation.
A network pharmacology approach to explore the mechanisms of action of Epimrdii Herba-Coicis Semen for treatment of Alzheimer's disease
Authors: Zhou Yuxuan 
Published: April 2020

Abstract
Background: Traditional Chinese medicine (TCM) can treat diseases through its “multi-component, multi-target, multi-pathway” mechanisms. Especially have advantages in the treatment of diseases with complicated pathogenesis, such as Alzheimer’s disease (AD). Tonifying the kidney and strengthening the spleen is one of the common methods of Chinese Medicine to treat AD. The TCM combination of Epimrdii Herba and Coicis Semen can be used as the main drugs of a prescription for tonifying the kidney and strengthening the spleen. However, the mechanisms for Epimrdii Herba-Coicis Semen (EH-CS) to treat AD is vague. The purpose of this study was to explore the mechanisms of EH-CS on AD using a network pharmacological method.Methods: We retrieved the chemical compounds and targets of Epimrdii Herba-Coicis Semen from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP). We screened the active ingredients based on the pharmacokinetic parameters (ADME). The Human Gene Database (GeenCards) was used to obtain disease targets of Alzheimer’s disease. Then we drew a venn diagram to obtain common targets of Chinese medicine and disease. Based on the topological properties, we screened the key targets. The protein-protein interaction (PPI) network was constructed using the STRING database, and the "Traditional Chinese Medicine-active ingredient-target" network was constructed using Cytoscape software. The key targets were respectively uploaded to the Metascape and DAVID database for GO and KEGG pathway analysis.Results: We obtained 31 active compounds for EH-CS. Flavonoids play important roles in the treatment of AD. A total of 29 key targets, including AKT1, MAPK1, and TP53, etc. The biological processes involve response to lipopolysaccharide, neuron death, neuroinflammatory response, etc. The main pathways include TNF signaling pathways, MAPK signaling pathways, PI3K-Akt signaling pathways and other signaling pathways.Conclusion: The network pharmacology method is an effective tool for exploring the mechanisms of TCM. Based on network pharmacology, this study systematically explained the potential mechanisms of EH-CS on AD. It provides a valuable reference for the development of AD drugs.