bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Social-Healthcare Pathway to Facilitate Return to Work of Cancer Survivors in Italy: Implementation Process of the UNAMANO project
Authors: Paltrinieri Sara , Ricchi Elena , Mazzini Elisa , Cervi Elena , Fugazzaro Stefania , Costi Stefania 
Published: November 2019

Abstract
Purpose Return to work (RTW) is a major goal to promote cancer survivors’ social participation. A multidisciplinary social-healthcare pathway called UNAMANO was designed to support RTW in this population. Methods UNAMANO was developed by the Azienda USL – IRCCS di Reggio Emilia (RE), in partnership with the local branch of the Italian Medical Association, nonprofit associations, vocational training institutions, social cooperatives, a labour union, and a chartered accounting firm. Results UNAMANO is directed towards employed individuals diagnosed with cancer living in the Province of RE. It has been implemented through four actions: A) healthcare professionals training on work-related occupational rehabilitation; B) dissemination among community and stakeholders; C) recruitment and training of volunteers; D) cancer survivor engagement and provision of a personalized comprehensive intervention. This consists in providing information and occupational therapy to overcome barriers and facilitate RTW or social support through re-training and tailored job search strategies for individuals based on their risk of job loss. Conclusions UNAMANO is the first Italian multidisciplinary social-healthcare pathway supporting RTW of cancer survivors. Addressing a wide area of cancer survivors’ needs, it provides personalized intervention to resolve work-related issues. We propose this patient-centred RTW model to promote an easier transition from hospital to community.Implications for cancer survivors UNAMANO can positively impact on cancer survivors’ health and well-being and can be provided at any stage of disease and treatment. UNAMANO ensures that RTW is coherent with cancer survivors’ health condition and expectations.
Antiviral effect of 1-Calcium phosphonate uracil against porcine reproductive and respiratory syndrome virus in vitro
Authors: Xu Lei , Xu Zhi wen , Jun Zhao , Yang Xiao yu , Zhang Ruo bo , Jiang Chao yuan , Yu Feng , Yin Xin huan , Li Fei , Huang Jianbo , Sun Xiangang , Ling Zhu 
Published: November 2019

Abstract
Background: Porcine reproductive and respiratory syndrome virus (PRRSV) is an RNA virus. At present, it has been treated by various ways, including vaccination of inactivated vaccine, attenuated live vaccine, subunit vaccine, etc., but the control of PRRSV infection is still inadequate Therefore, it is necessary to study new drugs against PRRSV infection. In this study, the antiviral effect of SR on PRRSV in vitro was studied.Results: PRRSV was significantly inhibited in SR pretreated cells. In addition, the results also showed that SR inhibited the adhesion, entry and synthesis of PRRSV, but did not affect the release of PRRSV.Conclusion: Our results suggest that SR may play an antiviral role in PRRSV infection in vitro, which suggests that SR may be a potential new drug to inhibit PRRSV infection
Comparing the effects of dezocine with lidocaine on prevention of propofol injection pain in adults: a prospective randomized double-blind trial
Authors: Zhu Xuqin , Jia Songlin , Xu Yajun , Sun Zhirong 
Published: November 2019

Abstract
Background Propofol injection pain (PIP) is common and may decrease patient comfort. The aim of this study was to compare the effects of dezocine with lidocaine on prevention of PIP.Methods 235 patients, who scheduled for elective surgery, aged 18–80 years and American Society of Anesthesiologists (ASA) I or II, were randomly assigned into five groups (n = 47 each). All patients were induced through the dor­sal hand vein or antecubital vein. The five groups were given the following medication intravenously: saline (Group I), lidocaine 20 mg (Group II), lidocaine 40 mg (Group III), dezocine 2 mg (Group IV) and dezocine 4 mg (Group V). Twenty seconds later all patients received a propofol infusion and were asked to grade pain or discomfort in the hand or forearm according to a four-point scale until anesthesia.Results Three groups showed a significantly lower incidence of total PIP than Group I: Group III (OR [Odds ratio]: 0.39 (0.16, 0.93)); Group IV (OR: 0.21 (0.09, 0.51)) and Group V (OR: 0.12 (0.05, 0.30)). Group IV showed a significantly lower incidence of total PIP than Group II (OR: 0.32 (0.13, 0.77)). Group V showed a significantly lower incidence of total PIP than Group II (OR: 0.15 (0.06, 0.39)) or Group III (OR: 0.30 (0.13, 0.72)).Conclusion Dezocine is a novel agent to alleviate PIP and appears to some extent to be more effective than lidocaine.
In‐Field Whole‐Plant Maize Architecture Characterized by Subcanopy Rovers and Latent Space Phenotyping
Authors: Gage Joseph L. , Richards Elliot , Lepak Nicholas , Kaczmar Nicholas , Soman Chinmay , Chowdhary Girish , Gore Michael A. , Buckler Edward S. 
Published: November 2019

Core Ideas
Subcanopy rovers enabled 3D characterization of thousands of hybrid maize plots.
Machine learning produces heritable latent traits that describe plant architecture.
Rover‐based phenotyping is far more efficient than manual phenotyping.
Latent phenotypes from rovers are ready for application to plant biology and breeding.
Collecting useful, interpretable, and biologically relevant phenotypes in a resource‐efficient manner is a bottleneck to plant breeding, genetic mapping, and genomic prediction. Autonomous and affordable subcanopy rovers are an efficient and scalable way to generate sensor‐based datasets of in‐field crop plants. Rovers equipped with lidar can produce three‐dimensional reconstructions of entire hybrid maize (Zea mays L.) fields. In this study, we collected 2103 lidar scans of hybrid maize field plots and extracted phenotypic data from them by latent space phenotyping. We performed latent space phenotyping by two methods, principal component analysis and a convolutional autoencoder, to extract meaningful, quantitative latent space phenotypes (LSPs) describing whole‐plant architecture and biomass distribution. The LSPs had heritabilities of up to 0.44, similar to some manually measured traits, indicating that they can be selected on or genetically mapped. Manually measured traits can be successfully predicted by using LSPs as explanatory variables in partial least squares regression, indicating that the LSPs contain biologically relevant information about plant architecture. These techniques can be used to assess crop architecture at a reduced cost and in an automated fashion for breeding, research, or extension purposes, as well as to create or inform crop growth models.
Naphthalene Toxicity in Clinical Practice
Authors: Pannu Ashok Kumar , Singla Vidhi 
Published: November 2019

Background:
Naphthalene ingestion and skin or inhalational exposure (accidental or deliberate) is an
under-recognized cause of a severe toxidrome in regions where it is commonly used (e.g., mothballs in households).

Methods:
This review is an update for the clinicians to understand the pharmacology, clinical features, laboratory
evaluation, and treatment for naphthalene toxicity. High-quality literature for the past eight decades was collected
and reviewed in this article. Several landmark articles were reviewed using PubMed, EMBASE Ovid, and the Cochrane
Library, which have essential implications in the current toxicology practice.

Results and Conclusion:
Naphthalene toxicity usually occurs abruptly and leads to acute hemolysis, methemoglobinemia,
renal failure, respiratory depression, and acute brain dysfunction that are difficult to manage. The toxicity is
more marked in patients with G6PD deficiency and associated with high morbidity and mortality. The management
should mainly focus on high-quality supportive care; however, severe methemoglobinemia (>20-30%) requires specific
therapy with intravenous methylene blue. Methylene blue is a highly effective agent but contraindicated in
severe G6PD deficiency.
Treatment Strategies Against Psoriasis: Principle, Perspectives and Practices
Authors: Ramanunny Arya Kadukkattil , Wadhwa Sheetu , Singh Sachin Kumar , Sharma Deep Shikha , Khursheed Rubiya , Awasthi Ankit 
Published: November 2019

Background:Psoriasis is a genetically predisposed autoimmune disease mediated by cytokines released by the activated immune cells. It manifests inflammatory, scaly red or white silvery flaky skin which may be a fluid-filled lesion with soreness and itchiness. The prevalence rate of psoriasis is increasing day by day. Despite having such a high prevalence rate, the treatment of psoriasis is still limited. Hence, there is a need to rethink the various treatment strategies available in the allopathic as well as in the alternative systems of medicine.Methods:Various bibliographic databases of previously published peer-reviewed research papers were explored and systematic data culminated in terms of various treatment strategies used for the management of psoriasis. The prime focus is given towards modern as well as alternative systems of medicine such as phototherapy, a combination of phototherapy with pharmacotherapy such as Ayurveda, Yoga and naturopathy, Unani, Siddha, and Homeopathy to treat psoriasis.Results:A comprehensive review of 161 papers, including both research and review articles, was carried out to make the article readily understandable. The pathogenesis including inflammatory mediators and type of psoriasis is discussed before the treatment strategies to understand the pathophysiology of the disease. The uniqueness, procedure, advantages, and limitations of conventional, advanced, and traditional systems of medicine to treat psoriasis are discussed in detail. Emphasis has also been given towards marine sources such as fish oil, marine sponges, and algae.Conclusion:Although there are many modern and alternative treatment strategies available to treat psoriasis, none of them have been proven to provide complete relief to patients. Moreover, they are associated with certain side effects. In order to overcome them, novel drug delivery systems have been utilized and found effective; however, their stability and safety become the major impediments towards their successful positioning. Traditional and alternative treatment strategies have found to be safe and effective but their use is localized to certain areas. In a nutshell, to achieve successful treatment of psoriasis, there is a need to focus on the development of stable and non-toxic novel drug delivery systems or the promotion of traditional systems to treat psoriasis.
Pharmacophore Modeling, Synthesis, Scaffold Hopping and Biological β- Hematin Inhibition Interaction Studies for Anti-malaria Compounds
Authors: Fayyazi Neda , Esmaeili Somayeh , Taheri Salman , Ribeiro Frederico F. , Scotti Marcus T. , Scotti Luciana , Ghasemi Jahan B. , Saghaei Lotfollah , Fassihi Afshin 
Published: November 2019

Backgound:Exploring potent compounds is critical to generating multi-target drug discovery. Hematin crystallization is an important mechanism of malaria.Methods:A series of chloroquine analogues were designed using a repositioning approach to develop new anticancer compounds. Protein-ligand interaction fingerprints and ADMET descriptors were used to assess docking performance in virtual screenings to design chloroquine hybrid β-hematin inhibitors. A PLS algorithm was applied to correlate the molecular descriptors to IC50 values. The modeling presented excellent predictive power with correlation coefficients for calibration and cross-validation of r2 = 0.93 and q2 = 0.72. Using the model, a series of 4-aminoquinlin hybrids were synthesized and evaluated for their biological activity as an external test series. These compounds were evaluated for cytotoxic cell lines and β-hematin inhibition.Results:The target compounds exhibited high β-hematin inhibition activity and were 3-9 times more active than the positive control. Furthermore, all the compounds exhibited moderate to high cytotoxic activity. The most potent compound in the dataset was docked with hemoglobin and its pharmacophore features were generated. These features were used as input to the Pharmit server for screening of six databases.Conclusion:The compound with the best score from ChEMBL was 2016904, previously reported as a VEGFR-2 inhibitor. The 11 compounds selected presented the best Gold scores with drug-like properties and can be used for drug development.
The Cytoprotective and Anti-cancer Potential of Bisbenzylisoquinoline Alkaloids from Nelumbo nucifera
Authors: Manogaran Prasath , Beeraka Narasimha Murthy , Padma Viswanadha Vijaya 
Published: November 2019

:
Natural product therapy has been gaining therapeutic importance against various diseases,
including cancer. The failure of chemotherapy due to its associated adverse effects promoted adjunct
therapy with natural products. Phytochemicals exert anti-carcinogenic activities through the regulation
of various cell signaling pathways such as cell survival, inflammation, apoptosis, autophagy and metastasis.
The ‘small molecule-chemosensitizing agents’ from plants induce apoptosis in drug-resistant and
host-immune resistant cancer cells in in vitro as well as in vivo models. For example, alkaloids from Nelumbo
nucifera, liensinine, isoliensinine and neferine exert the anticancer activity through enhanced
ROS generation, activation of MAP kinases, followed by induction of autophagy and apoptotic cell
death. Likewise, these alkaloids also exert their cytoprotective action against cerebrovascular
stroke/ischemic stroke, diabetes, and chemotherapy-induced cytotoxicity. Therefore, the present review
elucidates the pharmacological activities of these bisbenzylisoquinoline alkaloids which include the cytoprotective,
anticancer and chemosensitizing abilities against various diseases such as cardiovascular
diseases, neurological diseases and cancer.
The Cooperative Effect between Polybasic Region (PBR) and Polysialyltransferase Domain (PSTD) within Tumor-Target Polysialyltranseferase ST8Sia II
Authors: Zhou Guo-Ping , Liao Si-Ming , Chen Dong , Huang Ri-Bo 
Published: November 2019

:ST8Sia II (STX) is a highly homologous mammalian polysialyltransferase (polyST), which is a validated tumor-target in the treatment of cancer metastasis reliant on tumor cell polysialylation. PolyST catalyzes the synthesis of α2,8-polysialic acid (polySia) glycans by carrying out the activated CMP-Neu5Ac (Sia) to N- and O-linked oligosaccharide chains on acceptor glycoproteins. In this review article, we summarized the recent studies about intrinsic correlation of two polybasic domains, Polysialyltransferase domain (PSTD) and Polybasic region (PBR) within ST8Sia II molecule, and suggested that the critical amino acid residues within the PSTD and PBR motifs of ST8Sia II for polysialylation of Neural cell adhesion molecules (NCAM) are related to ST8Sia II activity. In addition, the conformational changes of the PSTD domain due to point mutations in the PBR or PSTD domain verified an intramolecular interaction between the PBR and the PSTD. These findings have been incorporated into Zhou’s NCAM polysialylation/cell migration model, which will provide new perspectives on drug research and development related to the tumor-target ST8Sia II.
Pulmonary Rehabilitation Effects on Heart Rate Recovery in Restrictive Lung Disease Patients
Authors: Ribeiro José Savio , da Silva Barros Darlé Ana , de Castro Antônio Adolfo Mattos , de Oliveira e Silva Natália Cristina Vargas , Kumpel Claudia , Porto Elias Ferreira 
Published: November 2019

Objective:
The study aimed to evaluate heart rate recovery in subjects with restrictive lung
disease (RLD) and healthy age matched subjects before and after a rehabilitation program (RP).

Methods:
This is a cross-sectional study on subjects of both genders, with over 40 years old with and
without any diagnosis of restrictive lung disease and who were able to perform physical tasks were
included in the study. They were evaluated for sociodemographic profile, lifestyle (ILP),
Framingham score, physical capacity (6MWT and treadmill incremental testing) and heart rate
recovery before and after RP.

Results:
65 subjects were assigned into either G1 (patients with RLD, n=26) or G2 (healthy subjects,
n= 39). Initially, patients with RLD increased their heart rate (HR) from 79 to 120bpm, and after the
recovery post-exercise, the first minute they reduced HR by nine beats. In the fifth minute after the
exercise, HR returned to baseline values. After the RP, mean HR was 71bpm and 79bpm in G1 and
G2, respectively. Increased mean 6MWT peak HR in both the groups was seen (110bpm and
120bpm, G1 and G2, respectively). In the first minute of resting, mean HR decreased to 86bpm (-
24bpm) and 72 (-48bpm) in G1 and G2, respectively. In the fifth minute after exercise, HR recovery
in both the groups was complete.

Conclusion:
The RP was effective in improving the first-minute heart rate recovery in patients with
restrictive lung disease and, there was an inverse correlation of heart rate recovery with disease
severity.
An Updated Overview of The Complex Clinical Spectrum of Tourette Syndrome
Authors: Gadoth Natan 
Published: November 2019

Background:
Tourette syndrome is reflectively and quite erroneously associated by
many as a syndrome with tics and swearing. However, the syndrome is a complex neuropsychiatric
disorder consisting of features known as Tourette syndrome combined with several serious comorbidities
justifying the quite recent term of Tourette disorder rather than “syndrome”. Unfortunately,
the published literature is mostly dedicated to tics, while mentioning the comorbidities only briefly.

Objective:
The study aimed to provide a brief description of the “many faces” of Tourette syndrome.

Methods:
This study included a summary of the relevant published literature.

Results:
The literature review provided indicates that this rare neuropsychiatric clinical syndrome is
more a multidisciplinary “disorder” rather than a” syndrome”.

Conclusion:
Patients with Tourette disorder should be evaluated and treated by a multidisciplinary
team.
The Pathophysiology of Tics; An Evolving Story
Authors: Singer Harvey S. , Augustine Farhan 
Published: November 2019

Background:Tics, defined as quick, rapid, sudden, recurrent, non-rhythmic motor movements or vocalizations are required components of Tourette Syndrome (TS) - a complex disorder characterized by the presence of fluctuating, chronic motor and vocal tics, and the presence of co-existing neuropsychological problems. Despite many advances, the underlying pathophysiology of tics/TS remains unknown.Objective:To address a variety of controversies surrounding the pathophysiology of TS. More specifically: 1) the configuration of circuits likely involved; 2) the role of inhibitory influences on motor control; 3) the classification of tics as either goal-directed or habitual behaviors; 4) the potential anatomical site of origin, e.g. cortex, striatum, thalamus, cerebellum, or other(s); and 5) the role of specific neurotransmitters (dopamine, glutamate, GABA, and others) as possible mechanisms (Abstract figure).Methods:Existing evidence from current clinical, basic science, and animal model studies are reviewed to provide: 1) an expanded understanding of individual components and the complex integration of the Cortico-Basal Ganglia-Thalamo-Cortical (CBGTC) circuit - the pathway involved with motor control; and 2) scientific data directly addressing each of the aforementioned controversies regarding pathways, inhibition, classification, anatomy, and neurotransmitters.Conclusion:Until a definitive pathophysiological mechanism is identified, one functional approach is to consider that a disruption anywhere within CBGTC circuitry, or a brain region inputting to the motor circuit, can lead to an aberrant message arriving at the primary motor cortex and enabling a tic. Pharmacologic modulation may be therapeutically beneficial, even though it might not be directed toward the primary abnormality.
Immunofluorescence Assay Using Monoclonal and Polyclonal Antibodies for Detection of Staphylococcal Enterotoxins A in Milk
Authors: Godjevargova Tzonka , Becheva Zlatina , Ivanov Yavor , Tchorbanov Andrey 
Published: November 2019

Objectives:

Staphylococcus aureus is a Gram-positive microorganism. S. aureus can grow in various foods and cause food poisoning by secreting enterotoxins. The most common enterotoxins involved in food poisoning are staphylococcal enterotoxin A and staphylococcal enterotoxin B, but Staphylococcal Enterotoxin A (SEA) is predominant. The main types of food contaminated with SEs are meat and meat products, poultry and eggs, milk and dairy products. The aim of this study was to develop a rapid and sensitive fluorescence immunoassay for detection of staphylococcal enterotoxin A in milk.

Methods:
Monoclonal and polyclonal antibodies for SEA were produced and characterized. Competitive fluorescence immunoassay based on Magnetic Nanoparticles (MNPs) was performed and optimized. MNPs were used as a solid carrier of the antibodies. The first step of the assay was immunoreaction between the immobilized antibody onto MNPs and SEA in milk sample. Then the fluorescein-SEA conjugate was added to the sample. Thus, competitive immunoreaction between MNP-mAb/MNP-pAb with SEA and SEA-FITC was performed. These immuno-complexes were separated by a magnetic separator and the obtained supernatants were analyzed. The fluorescent signal from the excess of conjugated SEA was proportional to the SEA contained in the milk. The assay duration was only 30 min.

Results:
The fluorescence immunoassays performed with polyclonal antibody had linear ranges from 5 pg/mL to 100 ng/mL SEA in a buffer, and from 50 pg/mL to 50 ng/mL SEA in spiked milk samples. While the same assays performed with monoclonal antibody had linear ranges from 1 pg/mL to 20 ng/mL SEA in buffer, and from 10 pg/mL to 10 ng/mL SEA in spiked milk samples. The detection limits of the developed immunoassays performed in milk were: 48 pg/mL with polyclonal antibody and 9 pg/mL with monoclonal antibody.

Conclusion:
A rapid and sensitive fluorescence immunoassay based on magnetic nanoparticles with a polyclonal and monoclonal antibody for determination of staphylococcal enterotoxin A in milk was developed.
Development of MNPs Based Enzyme Immuno-Sorbent Analysis for the Determination of Organophosphorus Pesticides in Milk
Authors: Ivanov Yavor 
Published: November 2019

Objectives:
High toxicity of pesticides requires accurate and reliable methods to monitor their levels for food, water and soil safety.

Methods:
Magnetic nanoparticles were synthesized and functionalized with (3-aminopropyl)triethoxysilane. Sheep polyclonal-anti-paraoxon and anti-dichlorvos antibodies were produced. Dichlorvos-cationized ovalbumin-HPR and paraoxon-ovalbumin-HPR conjugates were prepared and characterized. The optimal conditions for antibody immobilization were determined. Enzyme immunosorbent assay based on Magnetic Nanoparticles (MNPs) for the determination of pesticides was developed. A comparison of the developed modified MNPs-based ELISA with the conventional ELISA was performed. The effect of protein, pH, and fats in milk samples on the analytical characteristics of the MNPs-based ELISA was investigated. In order to validate the results obtained from the MNPs-based ELISA method, experiments with HPLC were performed.

Results:
The linear range of the standard curve for the determination of paraoxon in buffer with anti-paraoxon antibody was 0.125-5 ng/mL, for the dichlorvos with anti-dichlorvos antibody was 0.0625-5 ng/mL, and for the mixture of paraoxon and dichlorvos in ratio (1:1) with multi-antibody was 0.125-5 ng/mL. A comparison of the developed modified MNPs-based ELISA with the conventional ELISA was performed. The recovery of the obtained results for the determination of pesticides in milk samples in both methods was from 104 to 110%.

Conclusion:

The stated advantages of magnetic nanoparticles provide good sensitivity and rapidity of immunoassay and a simple procedure for separating of antibody-magnetic nanoparticles with the bound antigen from the non-bound antigen in the sample.