bluuebunny/crossref_metadata_2025_split huggingface dataset:
Practice of Modern Methods of Family Planning in Two Urban Slums of Karachi and Effect of Counselling
Authors: Akram Dure Samin , Khalid Farah , Hanif Yasmeen , Taj Sobia 
Published: November 2020

Abstract
BACKGROUNDThe means and services used for prevention of pregnancies is referred as Family Planning (FP) (1). The successful implementation of Family Planning has remained a difficult task in Pakistan because of socio-cultural beliefs, lack of information or misinformation and lack of access to contraceptive services. Cultural taboos and social bonds related to women are major factors in the low uptake of FP services (2). METHODOLOGThis study is the analysis of retrospective secondary data from an NGO, HELP’s Family Planning clinics, to determine the trends and patterns of contraceptive methods used in two squatter settlements(Neelam Colony and KKB) in Karachi from 2010-2018. The data originated from Mother and Child centers of HELP. Records of the monthly data collected by CHWs from their catchments areas were obtained and it was cumulated annually. Variables studied included number of women of childbearing age (CBA), number of married women of childbearing age, information about number of family planning users, number of babies born. The data also included different types of FP methods used by married couples. For analysis, the data was entered in Microsoft Excel and then exported to SPSS version 19. Mean and percentages, graphs and other graphics for all the variables were calculated and created through SPSS 19 and Excel.RESULTSThe Mean number of FP users were 43% in Neelum Colony and 48% at KKB. Similar data was reported in PDHS 2017-18 that 34% of married women in Pakistan were opting for methods of family planning (3). Use of Condoms was the most commonly used method of family planning in Neelum Colony and in KKB, recorded as being 36%, and 41% users respectively. The FP2020 reports that there are 34% condom users in Pakistan (4). Our study also found that that that the increase in use of FP was influenced by increased reminders and nudging techniques during counselling by CHWs. CONCLUSIONSTo progressively increase the practice of couples using modern FP methods, it is crucial that repeated messages giving correct scientific information regarding FP methods be given frequently. Acceptance of using FP has to be developed primarily in the male counterpart and therefore, male social mobilisers should form part of a team.
A Study on the Clinical Application of Greater Omental Pedicle Flap Transplantation to Correct Anterior Resection Syndrome in Patients With Low Rectal Cancer
Authors: qin haiquan , Meng Linghou , Huang Zigao , Liao Jiankun , Feng Yan , Luo Shanshan , Lai Hao , Tang Weizhong , Mo Xianwei 
Published: November 2020

Abstract
PurposeLow anterior resection syndrome (LARS) is the most common complication after total mesorectal excision (TME) in patients with low rectal cancer and has been a challenge in colorectal surgery that severely impacts the quality of life of patients. This study aimed to introduce a revised surgical procedure which could effectively maintain rectal compliance and significantly improve LARS after the operation.MethodsWe performed mesorectal reconstruction after routine Dixon-TME using greater omental pedicle flap transplantation in 11 patients with low rectal cancer (5 cases of preoperative neoadjuvant chemoradiotherapy, 5 cases of preoperative neoadjuvant chemotherapy, and 1 case of postoperative adjuvant chemotherapy), thereby simulating the initial anatomical structure of the mesorectum and significantly reducing the postoperative anterior resection syndrome. The lars precision syndrome assessment scale (LARSS) was used to access the LARS.ResultsAt 12 weeks after the 11 patients recovered from the anal defecation function, the average score on the LARS questionnaire was 25.5 ± 0.5 (mild). The average time at which anal function began to recover was 6.2 ± 2.6 weeks after surgery. The recovery was rapid, as the rectal and anal function of all patients generally returned to normal levels within 12 weeks, and the quality of life was close to that before surgery. ConclusionGreater omental flap transplantation can significantly improve LARS after Dixon-TME in patients with low rectal cancer.
The Novel Ferroptosis-related Gene Markers That Can Predict the Survival in Gastric Cancer Patients
Authors: Chen Bang , Xu Xin , Zhu ShaoFu , Yang ShiYi , Yang Kang , Jin MengYao , Wang Tianbin , Men Tao , Cao Guodong , Chen Bo 
Published: November 2020

Abstract
Background: Gastric cancer(GC) refers to malignant tumor that derived from gastric epithelial cells. Ferroptosis is another programmed cell demise mode that is Fe-dependent, unique concerning apoptosis, cell necrosis, and autophagy. Current research demonstrates that ferroptosis assumes a basic part of cancer biology. Extracellular matrix(ECM) has been confirmed to play an essential role in the proliferation, apoptosis, metabolism and differentiation of tumor cells. As an important component of the tumor microenvironment, ECM interacts with the immune microenvironment and affects tumor development and progression. Methods: GC data were downloaded from the TCGA database. Furthermore, 259 ferroptosis-related genes were acquired with the FerrDb database. COX regression analysis was used to screen ferroptosis-related genes related to GC's prognosis, and these genes constructed the prediction model. The risk score of the model and clinical data of GC were further analyzed to get the correlation between the model and the overall survival(OS) rate and clinicopathological features. Finally, GO and KEGG enrichment analysis was carried out on the genes of the model. To further analyze the correlation between the genes in the model and tumor immunity, ssGSEA was used to score immune cells and calculate immune-related pathways' activity quantitatively. Results: A prognosis model was constructed according to the 11 ferroptosis-related genes related to prognosis to predict the prognosis of GC patients better. According to univariate and multivariate, risk score can be regarded as an independent predictor.Conclusions: we identified 11 ferroptosis-related genes (NOX4, NOX5, SLC1A5, GLS2, MYB, TGFBR1, NF2, ZFP36, DUSP1, SLC1A4, SP1), which affected the prognosis of GC patients.
Systematic Expression Analysis of Zinc-finger Transcription Factor Family Reveals the Importance of Snai1 in Colorectal Carcinogenesis
Authors: Li Jianxin , Han Ting , Wang Xin , Wang Yinchun , Yang Qingqiang 
Published: November 2020

Abstract
Background: Epithelial-mesenchymal transition (EMT) has long been recognized as one of the most important processes involved in cancer cells metastasis. SNAI1, a member of zinc-finger transcriptional factor family, has been identified as an EMT inducer, but its role in human colorectal cancer (CRC) remains largely unclear.Methods: In the present study, we made a synthesis of the expression of zinc-finger transcription factor family and investigate the prognostic value of SNAI1 in human CRC by using a series of bioinformatic tools.Results: SNAI1 is the only member of zinc-finger transcription factor family that frequently overexpressed in CRC. In addition, overexpression of SNAI1 was remarkably associated with high tumor stage, N classification and M classification in patients with CRC. Patients with high SNAI1 expression had worse prognosis than those with low SNAI1 expression. Moreover, cox regression analysis showed that SNAI1 was an independent risk factor for overall survival in CRC patients. KEGG pathway analysis showed that SNAI1 enriched in pathways of Focal adhesion, PI3K-Akt signaling pathway and ECM-receptor interaction. GO analysis revealed that SNAI1 participated in processes of extracellular matrix organization, cell adhesion and collagen catabolic process. Eventually, co-expression analysis revealed that overexpression of SNAI1 was significantly correlated with Biglycan (BGN), indicating that they may cooperate in regulating the process of EMT, thereby inducing cancer cells metastasis.Conclusions: Our results demonstrated that SNAI1 is overexpressed in CRC and acts as an independent molecular marker for prognosis of CRC patients. SNAI1 is a potential biomarker for diagnosis, targeted therapy and prognostic evaluation of CRC.
Identification of Key Genes and Pathways in Colorectal Cancer by Integrated Bioinformatics Analysis
Authors: Wang Chaochao , Zhang Li , Hu Yingchun 
Published: November 2020

Abstract
PurposeIn order to understand the mechanism of colorectal cancer occurrence and development, we screened related core genes and provided new targets for clinical diagnosis and treatment of colorectal cancer.MethodsWe downloaded CRC-associated gene expression profile of GSE110233 from Gene Expression Omnibus (GEO) dataset. There were 26 samples in this dataset, all the differentially expressed genes (DEGs) with p<0.05 and fold change ≥1 or ≤-1 were identified. Gene ontology (GO) and "Kyoto Encyclopedia of Genes and Genomes" (KEGG) were used to search for these DEG enrichment methods. In addition, the protein-protein interaction (PPI) network was also used to construct visual interactions between proteins. At last, we used GEPIA to conduct the survival analysis 4 down-regulation and 8 up-regulation genes for clarify the potential effects on CRC.ResultsA total of 866 differentially expressed genes were obtained, including 360 up-regulated genes and 506 down-regulated genes. These genes were involve in Cell proliferation; Extracellular exosome; Protein binding; Chemokine activity. Genes were mainly involved in the KEGG pathway termed Cell cycle; PI3K-Akt signaling pathway; Mineral absorption; MicroRNAs in cancer; Cytokine-cytokine receptor interaction. We finally found 12 hubgenes by PPI connective degree whom named PRKACB, FGFR2, FGFR3, CKB, TIMP1, CCNA2, CCNB1, CDC20, CDC6, CCND1, CDK4 and CDK1.ConclusionBioinformatics is helpful for comprehensive and in-depth study of the occurrence and development mechanism of diseases, to screen possible core targets, and to provide a reliable basis for clinical diagnosis and treatment of colorectal cancer.
Real-World Study of Hepatic Hemangioma at a Single Center -- Data From 2008 to 2017
Authors: He Yongfei , Yang Chengkun , Liang Tianyi , Wei Yongguang , Wei Zhongliu , Mo Shutian , Chen Zijun , Liao Xiwen , Su Hao , Zhu Guangzhi , Han Chuangye , Lv Zili , Peng Tao 
Published: November 2020

Abstract
Objective: The objective of the present study was to investigate the clinical and epidemiological characteristics of hepatic hemangioma (HH) and the risk factors for complications associated with HH resection in a single center experience.Materials and methods: Clinical data of 5,266,670 outpatients and 246,419 individuals who were physically examined between 2016 and 2017 were used to evaluate epidemiological profiles of HH. A total of 1,981 patients hospitalized with HH between January 1, 2008 and January 1, 2018 were examined to identify relevant features associated with surgery. Results: The study found that HH was similar in male and female incidence, mostly at the age of 40, and the tumor was usually single and mostly located in the right lobe of the liver. No serious postoperative complications occurred, and the method of HH resection was related to the occurrence of postoperative complications.Conclusion: HH is a common benign disease of the liver. There are certain distribution rules in age, tumor size, and location, and laparoscopic HH resection that can reduce postoperative complications.
Statin Use is Associated With a Lower Risk of Recurrence After Curative Resection in BCLC Stage 0-a Hepatocellular Carcinoma
Authors: Yang Shih-Yu , Wang Chih-Chi , Chen Kuang-Den , Liu Yueh-Wei , Lin Chih-Che , Chuang Ching-Hui , Tsai Yu-Chieh , Yao Chih-Chien , Yen Yi-Hao , Hsiao Chang-Chun , Hu Tsung-Hui , Tsai Ming-Chao 
Published: November 2020

Abstract
BackgroundUse of statins is associated with a reduced risk of hepatocellular carcinoma (HCC). However, the effect of statin use on HCC recurrence is unclear. This study aimed to evaluate the effect of statin use on recurrence after curative resection among patients with HCC.MethodsWe retrospectively assessed 820 patients with Barcelona Clinic Liver Cancer (BCLC) stage 0 or A HCC who underwent primary resection between January 2001 and June 2016 at Kaohsiung Chang Gung Memorial Hospital. Exposure to statins was defined as use of a statin for at least 3 months before HCC recurrence. Factors that influenced overall survival (OS) and recurrence-free survival (RFS) were analyzed using Cox proportional hazards models.ResultsOf the 820 patients, 46 (5.6%) used statins (statin group) and 774 (94.4%) did not (non-statin group). During the mean follow-up of 76.5 months, 440 (53.7%) patients experienced recurrence and 146 (17.8%) patients died. The cumulative incidence of HCC recurrence was significantly lower in the statin group than the non-statin group (p = 0.001); OS was not significantly different between groups. In multivariate analysis, age (hazard ratio [HR]: 1.291; p = 0.010), liver cirrhosis (HR: 1.743; p < 0.001), diabetes (HR:1.418; p = 0.001), number of tumors (HR: 1.750; p < 0.001), tumor size (HR: 1.406; p = 0.004) and vascular invasion (HR: 1.659; p < 0.001) were independent risk factors for HCC recurrence, whereas statin use (HR: 0.354; p < 0.001) and antiviral therapy (HR: 0.613; p < 0.001) significantly reduced the risk of HCC recurrence. The statin group still had lower RFS than the non-statin group after one-to-four propensity score matching.Conclusion﻿Statins may exert a chemo-preventive effect on HCC recurrence after curative resection.
A Western Dietary Pattern is Related with Allergic Rhinitis in Young Women
Authors: Rezapour Hadis , Fereidouni Mohammad , Asadi Zahra , Saharkhiz Mansoore , Ayadilord Malaksima , Mahmoudzadeh Sara , Moghadam Mohammad Reza Shadmand Foumani , Ferns Gordon A. , Bahrami Afsane 
Published: November 2020

Abstract
Background: There has been an increasing prevalence of allergic disorders globally, and it may be useful to characterize the predisposing and protective factors for the development of allergy. Diet has been identified as one possible environmental factor implicated in the pathogenesis of allergic diseases. The goal of this study was to evaluate the associations between dietary patterns with the presence of allergies in a group of young women in East of Iran Methods: In total 181 female students enrolled in this study. Presence of allergic diseases including allergic rhinitis (AR), asthma and eczema was confirmed by an expert allergist. Information about dietary intake was collected by using a 65-item validated food frequency questionnaire. Exploratory factor analysis was used to evaluate the relationships among food/nutrition variables. Result: Multivariate odds ratio of having AR was 2.5 (95% CI:1.1-5.1) for the highest compared to lowest tertile of the Western dietary pattern score. But, no significant relationship was found between the traditional dietary pattern and AR, asthma or eczema. Conclusion: Our findings indicate a potential role of Western dietary pattern, characterized by being rich in dairy products, snack, nuts and sugar in the development of AR. Since diet is a modifiable impetus, the relationship between AR and Western dietary pattern may has a clinical application, particularly in those who has risk factors for developing AR.
Fast ISP Coding Mode Optimization Algorithm Based on CU Texture Complexity for VVC
Authors: Liu Zhi , Dong Mengjun , Guan XiaoHan , Zhang Mengmeng , Wang Ruoyu 
Published: November 2020

Abstract
In lately published video coding standard Versatile Video Coding (VVC/ H.266), the intra sub-partitions (ISP) coding mode is proposed. It is efficient for frames with rich texture, but less efficient for frames that are very flat or constant. In this paper, by comparing and analyzing the rate distortion cost (RD-cost) of coding unit (CU) with different texture features for using and not using ISP(No-ISP) coding mode, it is found that CUs with simple texture can get better coding performance in No-ISP coding mode. Based on this observations, a fast ISP coding mode optimization algorithm based on CU texture complexity is proposed, which aims to determine whether CU needs to use ISP coding mode in advance by calculating CU texture complexity, so as to reduce the computation complexity of ISP. The experimental results show that under All Intra (AI) configuration, the coding time can be reduced by 7%, while the BD-rate only increase by 0.09%.
Early life bacterial succession under different diet regime Atlantic salmon (Salmo salar L.)
Authors: Nikouli Eleni , Kormas Konstantinos , Jin Yang , Olsen Yngvar , Bakke Ingrid , Vadstein Olav 
Published: November 2020

Abstract
Backgound: The present study investigated the effect of different lipid source in the feed on the colonization and the bacterial succession in early life stages (fertilized eggs until 93 days post first feeding) of S. salar. The two diets used in this study, FD (fish oil based diet) and VD (vegetable oil based diet), were formulated to cover the fish nutritional requirements and except the lipid source the components were identical between them.Hindgut samples collected at 0, 35, 65 and 93 days post first feeding (dpff). Moreover, fertilized eggs, yolk sac larvae, rearing water and feed were also sampled in order to assess a possible contribution of their microbiota to the colonization of the gut. To analyze the composition of the bacterial communities, the Illumina MiSeq platform was used. Results: S. salar growth variables (mean wet weight and total length) did not differ significantly during the experiment (p> 0.05) across replicate tanks and between dietary treatments. The analysis of the 16S rDNA sequencing data revealed a total of 4548 unique OTUs, affiliated in 21 bacterial phyla. Proteobacteria, Firmicutes, Actinobacteria and Bacteroidetes were the dominant bacterial phyla. 13 OTUs were shared among all S. salar samples independent of life stage and diet treatment. Similarity percentages analysis (SIMPER) based on Bray–Curtis distance, showed that the average dissimilarity among the groups of the same life stages was 76.0%, whereas the average dissimilarity within groups of the same dietary treatment was 78.5% (FD) and 83.6% (VD). Conclusion: Feeding on either fish oil or vegetable oil-based diets, did not result in significant differences in the intestinal microbiota. The composition of gut microbiota did not differ significantly between the two dietary treatments, but changed with age, and each stage was characterized by different dominant bacteria. These OTUs are related to species that provide different functions and have been isolated from a variety of environments. Finally, this study revealed the occurrence of a core microbiota independent of the studied life stages and diet during the early life stages of Atlantic salmon.
Salivary Cortisol and Infrared Thermographic Ocular Temperature Use as Biomarkers during Endurance Competitions
Authors: Mira Monica Cardoso de , Lamy Elsa , Santos Rute , Williams Jane , Pinto Mafalda Vaz , Martins Pedro , Rodrigues Patrícia , Marlin David 
Published: November 2020

Abstract
Objective quantification of effort and distress during endurance rides through biomarkers could help manage competitions more effectively and monitor horse welfare through an evidence-based approach. This study aimed to determine if salivary cortisol (SC) and ocular temperature measured by infrared thermography (IRTOT) are related to the outcome in endurance competitions. Saliva was collected and IRTOT measured from 61 and 14 horses, respectively, competing at qualifier 40km and 80km rides at Pre-Inspection (PI) and Vet Gates (VG). The variation of the baseline SC at the PI (median±IQR=0.27ng/dl±0.36) into VG1 was abrupt (93-256% rise) and in the next VGs either decreased or rose at a very modest level. Less experienced horses in the 40km ride showed a significantly (p<0.05) higher IRTOT (median±IQR=35.7ºC±1.4) at the PI, than their counterparts in the 80Km ride (median±IQR=35. ºC ±1.5). Horses classifying in the Top5, in the 40 km ride category had significantly (p=0.05) higher SC levels (median±IQR=0.90ng/ml ±0.61) at the PI, than horses positioned from 10th position on (median±IQR = 0.16ng/ml ±0.40). A lower IRTOT in the PI was correlated with a better placement (p>0.05) and those in the Top5 (median±IQR = 33.9ºC ±0.0) had a higher variation (+10.65%) into the last VG. A 62% predictive value for elimination (80% sensibility and 82% specificity) where SC is higher than 0.23ng/ml is advanced. SC and IRTOT can be potentially used in association to characterise physical effort and emotional stress in endurance competitions, but its significance to performance has to be put in context with the competition level.
Detection of anti-SARS-CoV-2 antibodies in patients with rheumatoid arthritis.
Authors: Oka Shomi , Higuchi Takashi , Furukawa Hiroshi , Shimada Kota , Hashimoto Atsushi , Matsui Toshihiro , Tohma Shigeto 
Published: November 2020

Abstract
Objectives: The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes coronavirus disease 2019 (COVID-19) and the outbreak of COVID-19 was reported in December 2019 in Wuhan, China. Serological test is conducted to discriminate prior infection of SARS-CoV-2. The influence of auto-antibodies on the results of anti-SARS-CoV-2 antibodies was investigated in a few studies. Here, we investigated whether the results of anti-SARS-CoV-2 antibodies would be modified in patients with rheumatoid arthritis (RA). Methods: Patients with RA were recruited at Sagamihara National Hospital from July 2014 to October 2015 (n=38, 2014 cohort) and at Tokyo National Hospital from June to October 2020 (n=93, 2020 cohort). Anti-SARS-CoV-2 antibodies were measured in collected sera from these RA patients by electrochemiluminescence immunoassay (ECLIA) or immunochromatographic assay (ICA). Results: Anti-SARS-CoV-2 antibodies were not detected in all the samples form RA patients in both cohorts by the ECLIA. However, anti-SARS-CoV-2 antibodies were detected in the serum samples from three (7.9%) in 2014 cohort by the ICA and fifteen (16.1%) in 2020 cohort. The IgM rheumatoid factor levels were increased in RA patients with IgM anti-SARS-CoV-2 antibodies by ICA compared with RA without any anti-SARS-CoV-2 antibodies (mean ± standard deviation [IU/ml], 1223.0 ± 1308.7 vs. 503.6 ± 1947.2, P=0.0101). The levels of IgG rheumatoid factor were also upregulated in RA patients with IgM anti-SARS-CoV-2 antibodies by ICA (4.0 ± 0.7 vs. 2.4 ± 0.9, P=0.0013). Conclusion: The results of IgM anti-SARS-CoV-2 antibody by the ICA would be modified by IgM or IgG rheumatoid factors in RA patients.