bluuebunny/crossref_metadata_2025_split huggingface dataset:
Biomechanical evaluation of strategies for adjacent segment disease after lateral lumbar interbody fusion: is the extension of pedicle screws necessary?
Authors: Liang Ziyang , Cui Jianchao , Zhang Jiarui , He Jiahui , Tang Jingjing , Ren Hui , Ye Linqiang , Liang De , Jiang Xiaobing 
Published: January 2020

Abstract
Background: Adjacent segment disease (ASD) is a well-known complication after interbody fusion. Pedicle screw-rod revision possesses sufficient strength and rigidity. However, is a surgical segment with rigid fixation necessary for ASD reoperation? This study aimed to investigate the biomechanical effect of different instrumentation on lateral lumbar interbody fusion (LLIF) for ASD treatment. Methods: A validated L2~5 finite element (FE) model was modified for simulation. ASD was considered the level cranial to the upper-instrumented segment (L3/4). Bone graft fusion in LLIF with bilateral pedicle screw (BPS) fixation occurred at L4/5. The ASD segment for each group underwent a) LLIF + posterior extension of BPS, b) PLIF + posterior extension of BPS, c) LLIF + lateral screw, and d) stand-alone LLIF. The L3/4 range of motion (ROM), interbody cage stress and strain, screw-bone interface stress, cage-endplate interface stress, and L2/3 nucleus pulposus of intradiscal pressure (NP-IDP) analysis were calculated for comparisons among the four models. Results: All reconstructive models displayed decreased motion at L3/4. Under each loading condition, the difference was not significant between models a and b, which provided the maximum ROM reduction (73.8% to 97.7% and 68.3% to 98.4%, respectively). Model c also provided a significant ROM reduction (64.9% to 77.5%). Model d provided a minimal restriction of the ROM (18.3% to 90.1%), which exceeded that of model a by 13.1 times for flexion-extension, 10.3 times for lateral bending and 4.8 times for rotation. Model b generated greater cage stress than other models, particularly for flexion. The maximum displacement of the cage and the peak stress of the cage-endplate interface were found to be the highest in model d under all loading conditions. For the screw-bone interface, the stress was much greater with lateral instrumentation than with posterior instrumentation. Conclusions: Stand-alone LLIF is likely to have limited stability, particularly for lateral bending and axial rotation. Posterior extension of BPS can provide reliable stability and excellent protective effects on instrumentation and endplates. However, LLIF with the use of an in situ screw may be an alternative for ASD reoperation.
Effects of aquatic physical intervention on fall risk, working memory and hazard-perception as pedestrians in older people: a pilot trial
Authors: Nissim Michal , Livny Abigail , Barmatz Caroline , Tsarfaty Galia , Berner Yitshal , Sacher Yaron , Giron Jonathan , Ratzon Navah Z. 
Published: January 2020

Abstract

Background: Normal aging is associated with balance, mobility and working memory decline that increase fall risk and influence activity of daily living functions. Mounting evidence suggests that physical activity is beneficial for decreasing aging effects. Previous studies have focused on land-based physical activity. Research concerning the aquatic environment is scarce. The primary objectives of this three arm intervention pilot study were to examine the effects of an aquatic physical intervention program on balance, gait, fall risk and working memory among community-dwelling older individuals. The secondary objective was to examine the effects of an aquatic physical intervention program on safety of street–crossing among community-dwelling older individuals. Methods: Forty-two healthy participants aged 65 or older were enrolled into one of three intervention groups: aquatic physical intervention (API) (N=13), on-land physical intervention (OLPI) (N=14) or non-physical intervention (NPI) (N=15). The intervention took place from 2018 until 2019 at Tel-Aviv University, Sheba medical center and Reich Center. The protocol included 30-minute sessions twice a week for 12 weeks. Balance, gait and fall risk were assessed by the Tinneti test, working memory abilities were assessed by digit span and Corsi blocks tests and simulated safe streets-crossing was assessed by the hazard perception test for pedestrians.Testing and data collection was conducted at baseline, after six weeks and 12 weeks of intervention. All members of the professional team involved in evaluating participants were blind to the intervention group to which participants were allocated. Results: The differences in Tinetti balance (F(2,39)=10.03, p<0.01), fall risk (F(2,39)=5.62, p0>.05), digit span forward (F(2,39)=8.85, p<0.01) and Corsi blocks forward (F(2,39)=3.54, p<0.05) and backward (F(2,39)=6.50, p<0.05) scores after 12 weeks between the groups were significant. The API group showed improved scores. The differences in hazard perception test for pedestrians scores after 12 weeks of intervention between the groups were marginally significant (F(2,39)=3.13, p=0.055). The API group showed improved scores. Conclusions: These findings may affect experts working with the elderly population when making decisions concerning therapeutic prevention interventions for the deficiencies of elderly patients. Older adults practicing aquatic physical activity could contribute to their increased safety. Trial registrationTrial registration number: ClinicalTrials.gov Registry NCT03510377. Date of registration: 10/31/2017
KLF4 alleviates cerebral vascular injury by ameliorating vascular endothelial inflammation and regulating tight junction protein expression following ischemic stroke
Authors: Zhang Xinyu , Wang Lu , Han Zhenxiang , Dong Jing , Pang Defang , Fu Yuan , Li Longxuan 
Published: January 2020

Abstract
Background Although inflammatory cell adhesion molecules (CAMs) and anti-inflammation factor- kruppel-like transcription factor (KLF) 4 have all been reported to be induced after cerebral ischemic stroke (CIS), the close temporal and spatial relationship between expressions of CAMs and KLF4 following CIS, and whether and how CAMs and KLF-4 contribute to development of CIS-induced vascular injury are still unclear. Methods Here, we first examined the correlation between serum levels of CAMs/KLF4 and infarct volume in acute CIS patients. Then, we determined relationship between CAMs and KLF4 in mice after focal cerebral ischemia. Finally, we investigated the mechanism of KLF4 in protecting against oxygen-glucose deprivation-induced brain endothelial cell injury. Results Our results demonstrated that patients with moderate to severe CIS had higher serum levels of three CAMs including E-selectin, inter-cellular adhesion molecule 1 (ICAM-1) and vascular cell adhesion molecule 1 (VCAM-1) but lower levels of KLF4 at 48 hours after acute event as compared to patients with minor CIS. The expression levels of three CAMs as well as KLF4 all correlated well with the infarct volume in all the CIS subjects at that time. Although the expressions of three CAMs and KLF4 were all induced in the ischemic hemisphere following focal cerebral ischemia, but the peak timing and distribution patterns of their expression were different: the induction of KLF4 lagged behind that of the CAMs in the ischemic penumbra, furthermore, the dual immunofluorescent studies displayed that high expression of KLF4 was always associated with relatively less cerebral vascular endothelial inflammation response in the ischemic hemisphere, and vice versa. Mechanistic analyses revealed that KLF4 alleviated CIS-induced cerebral vascular injury by regulating endothelial expressions of CAMs, nuclear factor-kB and tight junction proteins. Conclusions These data indicate that KLF4 confers vascular protection against cerebral ischemic injury, suggesting that circulating CAMs and KLF4 might be used as potential biomarkers for predicting the prognosis of acute ischemic stroke, and also providing a new proof of concept and potential targets for future prevention and treatment of CIS.
Unveiling synapse pathology in spinal bulbar muscular atrophy by genome-wide transcriptome analysis of purified motor neurons derived from disease specific iPSCs
Authors: Onodera Kazunari , Shimojo Daisuke , Ishihara Yasuharu , Yano Masato , Miya Fuyuki , Banno Haruhiko , Kuzumaki Naoko , Ito Takuji , Okada Rina , Herculano Bruno de Araujo , Ohyama Manabu , Yoshida Mari , Tsunoda Tatsuhiko , Katsuno Masahisa , Doyu Manabu , Sobue Gen , Okano Hideyuki , Okada Yohei 
Published: January 2020

Abstract
Spinal bulbar muscular atrophy (SBMA) is an adult-onset, slowly progressive motor neuron disease caused by abnormal CAG repeat expansion in the androgen receptor (AR) gene. Although ligand (testosterone)-dependent mutant AR aggregation has been shown to play important roles in motor neuronal degeneration by the analyses of transgenic mice models and in vitro cell culture models, the underlying disease mechanisms remain to be fully elucidated because of the discrepancy between model mice and SBMA patients. Thus, novel human disease models that recapitulate SBMA patients’ pathology more accurately are required for more precise pathophysiological analysis and the development of novel therapeutics. Here, we established disease specific iPSCs from four SBMA patients, and differentiated them into spinal motor neurons. To investigate motor neuron specific pathology, we purified iPSC-derived motor neurons using flow cytometry and cell sorting based on the motor neuron specific reporter, HB9 e438 ::Venus , and proceeded to the genome-wide transcriptome analysis by RNA sequences. The results revealed the involvement of the pathology associated with synapses, epigenetics, and endoplasmic reticulum (ER) in SBMA. Notably, we demonstrated the involvement of the neuromuscular synapse via significant upregulation of Synaptotagmin, R-Spondin2 (RSPO2), and WNT ligands in motor neurons derived from SBMA patients, which are known to be associated with neuromuscular junction (NMJ) formation and acetylcholine receptor (AChR) clustering. These aberrant gene expression in neuromuscular synapses might represent a novel therapeutic target for SBMA.
Excellent multi-stakeholder acceptability and reimbursable costs in a pilot pragmatic trial of an evidence-based physical activity counseling intervention for patients with type 2 diabetes
Authors: Huebschmann Amy G. , Glasgow Russell E , Leavitt Ian M , Chapman Kristi , Rice John D , Lockhart Steven , Stevens-Lapsley Jennifer E , Reusch Jane EB , Dunn Andrea L , Regensteiner Judith G 
Published: January 2020

Abstract
Background: Physical activity (PA) improves important health outcomes for patients with type 2 diabetes mellitus (T2D), including physical function. We iteratively adapted the implementation strategies of pragmatic and evidence-based PA counseling programs to meet primary care stakeholders’ needs, resulting in the “Be ACTIVE” program. In a pilot randomized pragmatic trial, we evaluated the feasibility, acceptability and effectiveness of Be ACTIVE. Methods: Formative activities involved engaging multi-level stakeholders (patients, clinicians, coaches) to tailor implementation strategies for Be ACTIVE to the primary care context, while taking care to preserve the core “functions” of Be ACTIVE. Be ACTIVE included: a PA tracker (FitBit©), six theory-informed PA counseling phone calls, and three in-person clinician visits. Sedentary patients with T2D from two academic primary care clinics were randomized to Be ACTIVE vs. enhanced usual care. We used mixed methods to assess implementation outcomes of feasibility and acceptability among multi-level stakeholders, including costs. Objective effectiveness outcomes included PA (primary outcome, steps/week), physical function (secondary outcomes, including Short Physical Performance Battery (SPPB)), and behavioral PA predictors. Results: Multi-level stakeholders were engaged in formative activities to design a feasible pragmatic intervention. Fifty patients were randomized to Be ACTIVE or enhanced usual care. Acceptability was >90% for patients and clinic staff. In-person visits were fully reimbursed, and counseling costs of ~$90/patient would be reimbursable by Medicare. Pre-post PA increased by ~11% absolute in the Be ACTIVE group and by ~6% in controls (group difference: 1574 ± 4391 steps/week, p = 0.72) — less than the clinically important threshold of 4200 steps/week. Be ACTIVE participants’ physical function improved more than controls (SPPB: +0.9 ± 0.3 versus -0.1 ± 0.3, p = 0.01, changes >0.5 points are clinically important for preventing falls), and for PA predictors of self-efficacy (p=0.02) and social-environmental support (p<0.01). Conclusions: In this pilot trial, Be ACTIVE was feasible and highly acceptable to stakeholders and yielded significant improvements in objective physical function consistent with lower fall risk, while changes in PA were less than anticipated. Be ACTIVE may need adaptation or longer duration to clinically improve PA outcomes. Further optimizing the implementation strategies for sustainability is also needed.
Fouling of externally attached radio transmitters in an African river
Authors: Jacobs Francois Jakob , Weyl Olaf Lawrence Friedrich , Ulvan Eva Marita , Hay Clinton , Naesje Tor Fredrik 
Published: January 2020

Abstract
Fouling of externally attached tags is an important consideration in long term tagging studies as it may affect fish behaviour and well-being. Two externally attached radio transmitters on African tigerfish Hydrocynus vittatus were covered with short grown green algae, after the fish were recaptured 49 and 64 days after tagging in the Kavango River, Namibia. This is the first observation of fouling on external radio transmitters from any African river which highlights the importance of conducting studies that evaluate the various behavioural effects resulting from tagging.
Assessment of anti Rabies post-vaccinal immunity in domestic carnivores pets in low resources setting
Authors: Koffl Yao Mathurin , KOUAKOU Kouamé Casimir , N Kouamé Valère KOUAKOU , Kouadio Léonce , Gnabro Hugues Privat Godji , Assemian Krou Hermann , Kouassi Lou Sué Antoinette , COUACY-HYMANN Emmanuel 
Published: January 2020

Abstract
Objective Rabies is a zoonotic disease transmissible to humans mainly by dogs. It is a vaccine preventable disease. In Ivory Coast, canine rabies is a prioritary and notifiable animal disease since 1962. Evaluation of post-vaccination immunity against rabies was undertaken between 2003 and 2009 by an immunoenzymatic test Platelia Rabies II ELISA Kits (Bio-Rad). We aim to investigate anti Rabies vaccination efficacy in Ivory Coast, using data obtained purposely for antibody quantification prior to international movement of pets. Results A total of 527 dogs and cats sera were analyzed. Generally cats had developed higher level of post vaccinal immunity than dogs. We have found that more than 95.80% (114/119) of cats had a serum with antibodies titer above 0.5 EU /ml so like 74.75% (n = 305) of dogs. Undetectable or insufficient level of seroconversion was noted either in the canine specie (25.25%) or in cats (4.2%). Multivaccined Dogs, older than 4 years, represented the most important group (62.25%) of seronegative dog population. Associated with vaccination, serological results were used as data based-evidence in compliance with official requirements governing free movements of pets from rabies-infected to rabies-free european countries. Keywords : Ivory Coast, Rabies, ELISA, post-vaccinal immunity, dogs, cats
Effect of different drying temperatures on the rehydration of the fruiting bodies of Yu Muer (Auricularia cornea) and screening of browning inhibitors
Authors: Chen Yanqi , Lv Zhiwen , Liu Zhirun , Li Xiao , Li Changtian , Sossah Frederick Leo , Song Bing , Li Yu 
Published: January 2020

Abstract
In this study, the color of the dry fruiting bodies, drying ratio, amino acids, and total phenolics, which are of nutritional or commercial interest, were compared among different drying temperature treatments. The effect of rehydration methods and color protection reagents on the fruiting body-color, polyphenol oxidase (PPO) activity, and browning inhibition rate were evaluated. The results showed that drying with hot air at 65°C was quickest and resulted in a better color without compromising the drying ratio and rehydration ratio of the fruiting bodies. Furthermore, some reactions that occurred under high temperatures increased the content of protein, amino acids, and total phenolics. Soaking after boiling was the most suitable rehydration method, leading to the lowest PPO activity (39.87±1.35 U/g). All of the four analyzed color protection reagents could significantly inhibit the browning of Yu Muer fruiting bodies under room temperature water rehydration conditions.
Moving a neodymium magnet promotes migration of magnetic tracer and increases the monitoring counts on the skin surface of sentinel lymph node in breast cancer
Authors: Makita Masujiro , Manabe Eriko , Kurita Tomoko , Takei Hiroyuki , Nakamura Seigo , Kuwahata Akihiro , Sekino Masaki , Kusakabe Moriaki , Ohashi Yasuo 
Published: January 2020

Abstract
Background : We thought that moving a small neodymium magnet would promote migration of the magnetic tracer to the sentinel lymph node (SLN). Higher monitoring counts on the skin surface before incision help us detect SLNs easily and successfully. The aim of the present study was to verify enhancement of the monitoring count on the skin surface in SLN detection by the magnet movement in sentinel lymph node biopsy (SNB) using superparamagnetic iron oxide (SPIO) nanoparticles. Methods: After induction of general anesthesia, superparamagnetic iron oxide nanoparticles were injected sub-dermally into the subareolar area or peritumorally. The neodymium magnet was moved over the skin from the injection site to the axilla to promote migration of the magnetic tracer without massage. A total of 62 patients were enrolled from February 2018 to November 2018; 13 cases were given 20 times magnet movement (Group A), 8 cases were given 1-min magnet movement (Group B), 26 cases were given a short (about 5 min) interval from injection to 1-min magnet movement (Group C), and 15 cases were given a long (about 25 min) interval before 1-min magnet movement by the magnetometer’s head (Group D). In all cases, sentinel node biopsy was conducted using both the radioisotope (RI) and SPIO methods. The monitoring counts on the skin surface were measured by a handheld magnetometer and compared among the 4 groups. Changes in the monitoring count by interval and by magnet movement were evaluated. Results: The identification rates of the SPIO and RI methods were 100% and 95.2%, respectively. The mean monitoring counts of Group A, B, C, and D were 2.39 μT, 2.73 μT, 3.15 μT, and 3.92μT, respectively (p<0.0001; Kruskal-Wallis test) . The monitoring counts were higher with longer magnet movement and with the insertion of an interval. Although there were no relationships between the monitoring count on the skin surface and clinicopathologic factors, magnet movement influenced strongly on the monitoring count on the skin surface. Conclusion: Moving a small neodymium magnet is effective for promoting migration of a magnetic tracer and increasing monitoring counts on the skin surface.
The Combination of Serum Biomarker (CA-125, FASN, and GLS) as a Predictor Suboptimal Cytoreduction in Epithelial Ovarian Cancer
Authors: Winarno Gatot Nyarumenteng Adhipurnawan , Hidayat Yudi Mulyana , Soetopo Setiawan , Krisnadi Sofie Rifayani , Tobing Maringan Diapari Lumban , Rauf Syahrul 
Published: January 2020

Abstract
Purpose. Cytoreduction has an important role in improving the survival rate of epithelial ovarian cancer (EOC) patients. The use of preoperative CA-125 as an optimal predictor cytoreduction in patients with ovarian cancer is still controversial. This study aimed to assess the ability of preoperative serum CA125, FASN and GLS as a predictor of cytoreductive surgery in epithelial ovarian cancer (EOC). This observational-analytic cross-sectional study included 109 women diagnosed with epithelial ovarian cancer (EOC) between 2017-2019, who had serum CA-125, GLS, FASN measured preoperatively and underwent cytoreductive surgery.
Result. The average value of serum CA-125, FASN, and GLS in the suboptimal cytoreduction were higher than the optimal cytoreduction group. The cut off point (COP) of CA-125 was 248.55 (p=0.0001) with 73.2% sensitivity and 73.6% specificity, FASN was 0.445 (p=0.017) with 62.5% sensitivity and 60.4% specificity, and GLS was 22.895 (p=0.0001) with 73.2% sensitivity and 75.5% specificity. The COP value of CA-125 and GLS combined was 29.16 (p=0.0001) with sensitivity 82.1% and spesificity 73.6%, while the COP of CA-125, GLS, and FASN combined was 0.83 (p=0.0001) with 87.5% sensitivity and 73.6% specificity. If the value of biomarker serum more than COP will more likely have suboptimal cytoreductive surgery.
Conclusion. The role of CA125, FASN and GLS levels in predicting suboptimal cytoreductive surgery for patients with ovarian cancer seems questionable. However, the combination of CA-125 and GLS or CA-125, FASN and GLS are able to increase the sensitivity, specificity, and accuracy classification to predict suboptimal cytoreductive surgery.
Clostridioides difficile ribotype distribution in a large teaching hospital in Serbia
Authors: Korać Miloš , Rupnik Maja , Nikolić Nataša , Jovanović Milica , Tošić Tanja , Malinić Jovan , Mitrović Nikola , Marković Marko , Vujović Ankica , Peruničić Sanja , Bojović Ksenija , Djordjević Vladimir , Barać Aleksandra , Milošević Ivana 
Published: January 2020

Abstract
Background: The global epidemic of nosocomial diarrhea caused by Clostridioides ( Clostridium) difficile started in 2003, with high mortality rates and emergence of a new hypervirulent strain NAP1/BI/027. The aim of this study was to assess the presence of ribotype 027 and other C. difficile ribotypes in a Serbian University Hospital, to compare the temporal variability of ribotypes three years apart, as well as to compare clinical, demographic and laboratory characteristics and disease outcome among patients infected with 027 and non-027 ribotype. This was a prospective observational study addressing four months time intervals during 2014/2015 and 2017/2018. Results: Ribotyping was performed in 64 non-duplicate C.difficile strains. Ribotype 027 was the most prevalent, and was detected in 53 (82.8%) patients (43/45 and 10/19 patients in 2014-2015 and 2017/2018, respectively). Other detected ribotypes were 001/072 in 4 (6,3%), 002 in 4 (6,3%), 014/020 in 2 (3,1%) and 176 in 1 (1,5%) patient. The percentage of patients infected with ribotype 027 significantly decreased during the three-year period, from 95.6% to 52.6% ( p <0.001). Ribotype 027 infection was associated with fluoroquinolone treatment more frequently than infection with other ribotypes [33 (62.3%) vs. 2 (18.2%), p= 0.010)]. Severe C.difficile infection was diagnosed more often in patients with detected ribotype 027 compared to those infected with non-027 ribotypes (p=0.006). No significant difference in the mortality and recurrence rates was found between patients infected with ribotype 027 and those infected with other ribotypes [10/53 (18.8%) vs. 2/11 (18.2%), p=0.708, and 10/35 (28.6%) vs. 0/2 (0%), p=1.000, respectively]. Conclusion: C. difficile ribotype 027 was the most prevalent ribotype among patients in a large Serbian hospital, but there is a clear decreasing trend.