bluuebunny/crossref_metadata_2025_split huggingface dataset:
Compensatory ion transport buffers daily protein rhythms to regulate osmotic balance and cellular physiology
Authors: Stangherlin Alessandra , Wong David C. S. , Barbiero Silvia , Watson Joseph L. , Zeng Aiwei , Seinkmane Estere , Chew Sew Peak , Beale Andrew D. , Hayter Edward A. , Guna Alina , Inglis Alison J. , Bartolami Eline , Matile Stefan , Lequeux Nicolas , Pons Thomas , Day Jason , van Ooijen Gerben , Voorhees Rebecca M. , Bechtold David A. , Derivery Emmanuel , Edgar Rachel S. , Newham Peter , O’Neill John S. 
Published: May 2020

AbstractBetween 6-20% of the cellular proteome is under circadian control to tune cell function with cycles of environmental change. For cell viability, and to maintain volume within narrow limits, the osmotic pressure exerted by changes in the soluble proteome must be compensated. The mechanisms and consequences underlying compensation are not known. Here, we show in cultured mammalian cells andin vivothat compensation requires electroneutral active transport of Na+, K+, and Cl−through differential activity of SLC12A family cotransporters. In cardiomyocytesex vivoandin vivo, compensatory ion fluxes alter their electrical activity at different times of the day. Perturbation of soluble protein abundance has commensurate effects on ion composition and cellular function across the circadian cycle. Thus, circadian regulation of the proteome impacts ion homeostasis with substantial consequences for the physiology of electrically active cells such as cardiomyocytes.
Incremental housing: harnessing informality at Villa Verde
Authors: O'Brien David , Carrasco Sandra , Dovey Kim 
Published: May 2020

PurposeThis paper analyses the incremental housing process developed at Villa Verde, a housing project designed by the Chilean architecture firm Elemental, whose director Alejandro Aravena received the Pritzker Prize in 2016. This project is conceived within a social housing framework and designed as an affordable “half-house” to be incrementally extended by the owners.Design/methodology/approachThis paper is based on research undertaken in August 2017 with data obtained through site surveys, trace analysis, interviews with 32 residents and photographic surveys. The researchers mapped the modifications made by all households at Villa Verde in the four years after occupation.FindingsThe strategy of designing a formal framework for informal additions has generally been successful with most houses undergoing substantial expansion to a high standard of construction. The paper raises concerns regarding the settlement's urban design, response to local climate and the quality of shared open space. We also find evidence of over-development as informal additions extend across front and rear yards that are in some cases fully enclosed.Originality/valueThis project is critiqued within the context of a long series of architectural attempts to harness the productive capacities of self-help housing. Villa Verde engages the freedom to build in a self-organised manner within a formal framework. But what will stop these additions from escalating into a “slum”?
MHD slip flow and convective heat transfer due to a moving plate with effects of variable viscosity and thermal conductivity
Authors: Nandeppanavar Mahantesh M. , Kemparaju M.C. , Madhusudhan R. , Vaishali S. 
Published: May 2020

PurposeThe steady two-dimensional laminar boundary layer flow, heat and mass transfer over a flat plate with convective surface heat flux was considered. The governing nonlinear partial differential equations were transformed into a system of nonlinear ordinary differential equations and then solved numerically by Runge–Kutta method with the most efficient shooting technique. Then, the effect of variable viscosity and variable thermal conductivity on the fluid flow with thermal radiation effects and viscous dissipation was studied. Velocity, temperature and concentration profiles respectively were plotted for various values of pertinent parameters. It was found that the momentum slip acts as a boost for enhancement of the velocity profile in the boundary layer region, whereas temperature and concentration profiles decelerate with the momentum slip.Design/methodology/approachNumerical Solution is applied to find the solution of the boundary value problem.FindingsVelocity, heat transfer analysis is done with comparing earlier results for some standard cases.Originality/value100
Understanding online community participation behavior and perceived benefits: a social exchange theory perspective
Authors: Jahan Nusrat , Kim Seung Woon 
Published: May 2020

Purpose
Online communities (OCs) are the popular social environments in which people interact by sharing resources such as information, advice and thoughts on their mutual interests. Existing research lacks an explanation of the reasons of participation behavior in OCs and how such participation behavior provides members with perceived benefits. This study aims to observe how social exchange theory constructs (perceived members’ support and exchange ideology) affect online community participation behavior (OCPB), and moderated by exchange ideology (EI) and perceived ties, which in turn brings perceived benefits to its participants.

Design/methodology/approach
A survey method was followed to collect data, and structural equation modeling is used with 305 valid samples.

Findings
The results highlight the significant effects of perceived members’ support on OCPB. Participation behavior in OCs has critical effect on perceived benefits. The results also identify the moderating effects of EI and perceived tie, where perceived tie was insignificant.

Originality/value
The findings from this study bridge the literature gaps in the context of OCPB by demonstrating how practitioners and OC managers can enhance perceived members’ support, which result in OCPB, and thus provide the OC users with several perceived benefits.
Tests in Short Supply? Try Group Testing
Authors: Bilder Christopher R. , Iwen Peter C. , Abdalhamid Baha , Tebbs Joshua M. , McMahan Christopher S. 
Published: May 2020

AbstractChristopher R. Bilder, Peter C. Iwen, Baha Abdalhamid, Joshua M. Tebbs and Christopher S. McMahan explain how, by pooling specimens, testing capacity for SARS-CoV-2 can be increased
Comparing diversity levels in environmental samples: DNA sequence capture and metabarcoding approaches using 18S and COI genes
Authors: Giebner Hendrik , Langen Kathrin , Bourlat Sarah J. , Kukowka Sandra , Mayer Christoph , Astrin Jonas J. , Misof Bernhard , Fonseca Vera G. 
Published: May 2020

AbstractEnvironmental DNA studies targeting multiple taxa using metabarcoding provide remarkable insights into levels of species diversity in any habitat. The main drawbacks are the presence of primer bias and difficulty in identifying rare species. We tested a DNA sequence‐capture method in parallel with the metabarcoding approach to reveal possible advantages of one method over the other. Both approaches were performed using the same eDNA samples and the same 18S and COI regions, followed by high throughput sequencing. Metabarcoded eDNA libraries were PCR amplified with one primer pair from 18S and COI genes. DNA sequence‐capture libraries were enriched with 3,639 baits targeting the same gene regions. We tested amplicon sequence variants (ASVs) and operational taxonomic units (OTUs) in silico approaches for both markers and methods, using for this purpose the metabarcoding data set. ASVs methods uncovered more species for the COI gene, whereas the opposite occurred for the 18S gene, suggesting that clustering reads into OTUs could bias diversity richness especially using 18S with relaxed thresholds. Additionally, metabarcoding and DNA sequence‐capture recovered 80%–90% of the control sample species. DNA sequence‐capture was 8x more expensive, nonetheless it identified 1.5x more species for COI and 13x more genera for 18S than metabarcoding. Both approaches offer reliable results, sharing ca. 40% species and 72% families and retrieve more taxa when nuclear and mitochondrial markers are combined. eDNA metabarcoding is quite well established and low‐cost, whereas DNA‐sequence capture for biodiversity assessment is still in its infancy, is more time‐consuming but provides more taxonomic assignments.
New Lobellini (Collembola: Neanuridae) from Queensland contribute to understanding distribution and ecology of Australian fauna
Authors: Smolis Adrian , Greenslade Penelope 
Published: May 2020

AbstractTwo new species from Queensland rainforest belonging to genera Hemilobella and Sphaeronura are described and illustrated with drawings and colour photographs. Hemilobella matildae sp. nov. Smolis and Greenslade is characterised by elongated tubercles laterally on body and an elongated ogival labrum. Sphaeronura ameliae sp. nov. Smolis and Greenslade differs from other taxa in the genus in chaetotaxic features and the presence of a male ventral organ. A new record of Australonura scoparia is included. The high species richness of lowland rainforests, in which these log inhabiting saproxylic species were found, is noted, and the contribution that extreme events such as cyclones in maintaining the fallen timber habitat is emphasised. The bright colour of these species may be caused by sequestered pigments from the slime moulds on which they feed. The colour probably also acts as a warning to predators that individuals are distasteful. A description of the distributions, characteristics, distinctiveness and possible genesis of Australian fauna of the subfamily Neanurinae is presented, and a checklist of species and key to all Australian genera of the subfamily is included. Species in this subfamily are predominately short‐range endemics so should be surveyed whenever wet forests are under threat from logging or urban expansion.
The curly coat phenotype of the Ural Rex feline breed is associated with a mutation in the lipase H gene
Authors: Manakhov A. D. , Andreeva T. V. , Rogaev E. I. 
Published: May 2020

SummaryMutations in lipase H (LIPH) and lysophosphatidic acid receptor 6 (LPAR6), which are essential for the lysophosphatidic acid (LPA) signalling pathway, are associated with hypotrichosis and wooly hair in humans. Mutations in LPAR6 and keratin 71 (KRT71), result in unusual fur growth and hair structure in several cat breeds (Cornish Rex, Devon Rex and Selkirk Rex). Here, we performed target sequencing of the LIPH, LPAR6 and KRT71 genes in six cat breeds with specific hair‐growth phenotypes. A LIPH genetic variant (LIPH:c.478_483del; LIPH:p.Ser160_Gly161del) was found in Ural Rex cats with curly coats from Russia, but was absent in all other cat breeds tested. In silico three‐dimensional analysis of the LIPH mutant protein revealed a contraction of the α3‐helix structure in the enzyme phospholipid binding site that may affect its activity.
Temporo‐spatial and kinetic gait parameters in English setter dogs
Authors: Gündemir Ozan , Duro Sokol , Aydın Kaya Didar , Zenginler Yazgan Yonca 
Published: May 2020

AbstractWalking analysis systems have begun to be used in veterinary medicine in recent years. The pressure‐sensitive walkway is one of the systems through which we can obtain temporo‐spatial and kinetic variables of walking. Therefore, the aim of this study was to investigate the walking characteristics of English Setter dogs using a pressure‐sensitive system. Twenty‐five English Setter dogs were included in the study. Temporo‐spatial and kinetic gait parameters were obtained with the pressure‐sensitive walkway system. Centre of pressure values were taken separately for the forelimbs and hindlimbs and were statistically analysed. The force values in the forelimb were found to be greater than in the hindlimb during walking. According to the results of dynamic pedobarographic evaluation, the highest‐pressure values were found at the 2nd and 3rd digital pads for the forelimbs and on the 3rd and 4th digital pads for the hindlimbs. During the stance, 64.58% of the weight was found to be on the forelimbs. No difference was found between the forelimbs and the hindlimbs in centre of pressure analysis. As conclusion, the gait data obtained from the English Setter dogs can be used in future research to identify animals that may have neurological or orthopaedic problems.
Systematic review with meta‐analysis: efficacy and safety of treatments for opioid‐induced constipation
Authors: Vijayvargiya Priya , Camilleri Michael , Vijayvargiya Pooja , Erwin Patricia , Murad M. Hassan 
Published: May 2020

SummaryBackgroundWhen opioid‐induced constipation is treated with centrally acting opioid antagonists, there may be opioid withdrawal or aggravation of pain due to inhibition of μ‐opioid analgesia. This led to the development of peripherally acting μ‐opioid receptor antagonists (PAMORAs).AimTo evaluate the efficacy of available PAMORAs and other approved or experimental treatments for relieving constipation in patients with opioid‐induced constipation, based on a systematic review and meta‐analysis of published studies.MethodsA search of MEDLINE, EMBASE and EBM Reviews Cochrane Central Register of Controlled Trials was completed in July 2019 for randomised trials compared to placebo. FDA approved doses or highest studied dose was evaluated. Efficacy was based on diverse endpoints, including continuous variables (the bowel function index, number of spontaneous bowel movements and stool consistency based on Bristol Stool Form Scale), or responder analysis (combination of >3 spontaneous bowel movements or complete spontaneous bowel movements plus 1 spontaneous bowel movement or complete spontaneous bowel movements, respectively, over baseline [so‐called FDA endpoints]). Adverse effects evaluated included central opioid withdrawal, serious adverse events, abdominal pain and diarrhoea.ResultsWe included 35 trials at low risk of bias enrolling 13 566 patients. All PAMORAs demonstrated efficacy on diverse patient response endpoints. There was greater efficacy with approved doses of the PAMORAs (methylnaltrexone, naloxegol and naldemidine), with lower efficacy or lower efficacy and greater adverse effects with combination oxycodone with naloxone, lubiprostone and linaclotide.ConclusionsTherapeutic response in opioid‐induced constipation is best achieved with the PAMORAs, methylnaltrexone, naloxegol and naldemidine, which are associated with low risk of serious adverse events.
Hypoxia‐induced pulmonary hypertension—Utilizing experiments of nature
Authors: Gassmann Max , Cowburn Andrew , Gu Hong , Li Jia , Rodriguez Marisela , Babicheva Aleksandra , Jain Pritesh P. , Xiong Mingmei , Gassmann Norina N. , Yuan Jason X.‐J. , Wilkins Martin R. , Zhao Lan 
Published: May 2020

An increase in pulmonary artery pressure is a common observation in adult mammals exposed to global alveolar hypoxia. It is considered a maladaptive response that places an increased workload on the right ventricle. The mechanisms initiating and maintaining the elevated pressure are of considerable interest in understanding pulmonary vascular homeostasis. There is an expectation that identifying the key molecules in the integrated vascular response to hypoxia will inform potential drug targets. One strategy is to take advantage of experiments of nature, specifically, to understand the genetic basis for the inter‐individual variation in the pulmonary vascular response to acute and chronic hypoxia. To date, detailed phenotyping of highlanders has focused on haematocrit and oxygen saturation rather than cardiovascular phenotypes. This review explores what we can learn from those studies with respect to the pulmonary circulation.LINKED ARTICLESThis article is part of a themed issue on Risk factors, comorbidities, and comedications in cardioprotection. To view the other articles in this section visithttp://onlinelibrary.wiley.com/doi/10.1111/bph.v178.1/issuetoc
Mechanism underlying the development of myeloproliferative neoplasms through mutant calreticulin
Authors: Edahiro Yoko , Araki Marito , Komatsu Norio 
Published: May 2020

AbstractDeregulation of cytokine signaling is frequently associated with various pathological conditions, including malignancies. In patients with myeloproliferative neoplasms (MPNs), recurrent somatic mutations in the calreticulin (CALR) gene, which encodes a molecular chaperone that resides in the endoplasmic reticulum, have been reported. Studies have defined mutant CALR as an oncogene promoting the development of MPN, and deciphered a novel molecular mechanism by which mutant CALR constitutively activates thrombopoietin receptor MPL and its downstream molecules to induce cellular transformation. The mechanism of interaction and activation of MPL by mutant CALR is unique, not only due to the latter forming a homomultimeric complex through a novel mutant‐specific sequence generated by frameshift mutation, but also for its ability to interact with immature asparagine‐linked glycan for eventual engagement with immature MPL in the endoplasmic reticulum. The complex formed between mutant CALR and MPL is then transported to the cell surface, where it induces constitutive activation of downstream kinase JAK2 bound to MPL. Refined structural and cell biological studies can provide an in‐depth understanding of this unusual mechanism of receptor activation by a mutant molecular chaperone. Mutant CALR is also involved in modulation of the immune response, transcription, and intracellular homeostasis, which could contribute to the development of MPN. In the present article, we comprehensively review the current understanding of the underlying molecular mechanisms for mutant molecular chaperone‐induced cellular transformation.
Comparison of four oxidants activated through tetraacetylethylenediamine for developing sustainable and rapid degradation of organic dye
Authors: Dong Yongchun , Bian Liran , Zhang Congcan , Li Bing 
Published: May 2020

AbstractFour common oxidants, including hydrogen peroxide (H2O2), sodium percarbonate (SPC), sodium perborate (SPB) and sodium persulphate (SPS), were activated with tetraacetylethylenediamine (TAED) to degrade an azo dye, CI Reactive Red 195, in water, for building a novel and rapid oxidative system comprising the merits of cost‐effectiveness and high sustainability. Elevated temperature and high pH level enhanced the activation effect of TAED for accelerating dye degradation. Peracetic acids were confirmed to be the main oxidative species for dye degradation in four TAED/oxidant systems. Hydroxyl radicals and sulphate radicals were also involved in dye degradation in the TAED/SPS system, which showed a stronger oxidative capacity than the other three systems over a wide pH range. More importantly, the addition of inorganic salts or surfactants also favoured the dye degradation in TAED/oxidant systems. Although a slow mineralisation process of the dye was found when the TAED/SPC or SPS system was used, low‐toxic intermediates were detected after the degradation.
Patterns of alimentary resource use by dung beetles in introduced Brazilian pastures: Cattle versus sheep dung
Authors: Correa César M. A. , Peres Nathália D. , Holdbrook Robert 
Published: May 2020

AbstractDung beetles (Coleoptera: Scarabaeidae) perform essential ecological functions in pastures, such as dung removal, nutrient recycling and parasite control. However, the patterns of alimentary use by dung beetles in introduced Brazilian pastures are poorly known. Here, we compared dung beetle species richness, abundance and species composition in cattle and sheep dung, and identified the dung beetle species preference by each dung type. In January 2019, dung beetles were sampled with pitfall traps baited with cattle and sheep dung in 12 introduced pastures (Urochloa spp.), in Aquidauana, Mato Grosso do Sul, Brazil. A total of 592 individuals belonging to 14 species of dung beetles were collected. Of the 14 species sampled, nine were recorded in both dung types, five were found exclusively in sheep dung and no species was exclusive to cattle dung. Species richness and abundance were higher in sheep dung. Species composition was different between the dung types. Dichotomius bos (Blanchard), Genieridium bidens (Balthasar), Onthophagus aeneus Blanchard and Trichillum externepunctatum Preudhomme de Borre were associated with sheep dung. Our results provide evidence that sheep dung is more attractive to dung beetles with a distinct community species between the two dung types, although the studied pastures have never been used before for sheep breeding. Thus, our data shows that the introduction of a new alimentary resource (e.g. sheep dung) can be an important strategy to help to obtain a more diverse dung beetle assemblage in introduced Brazilian pastures.
Biogeography of soil microbial habitats across France
Authors: Karimi Battle , Villerd Jean , Dequiedt Samuel , Terrat Sébastien , Chemidlin‐Prévost Bouré Nicolas , Djemiel Christophe , Lelièvre Mélanie , Tripied Julie , Nowak Virginie , Saby Nicolas P. A. , Bispo Antonio , Jolivet Claudy , Arrouays Dominique , Wincker Patrick , Cruaud Corinne , Ranjard Lionel 
Published: May 2020

AbstractAimIntensive studies since the beginning of the 21st century have provided vast amounts of knowledge about soil microbial diversity at local and global scales. However, microbial habitats have been poorly investigated at large scale. This study aims to characterize soil bacterial habitats across France for the first time by integrating the description of numerous environmental factors and human activities.LocationWe focus on the large spatial scale of mainland France using the largest spatially explicit soil sampling set available across France (2,173 soils, area = 5.5 × 105 km2).Major taxa studiedSoil bacteria and archaea were studied by a high throughput sequencing approach targeting the V3‐V4 region of the 16S ribosomal ribonucleic acid (rRNA) gene directly amplified from soil DNA.MethodsWe applied decision tree learning and geostatistical approaches combining the abundant data on soil microbes and large‐scale environmental description in order to conduct a comprehensive analysis of soil bacterial and archaeal communities.ResultsWe identified a complex mosaic of 16 distinct terrestrial habitats, based on soil type and management (pH, C : N ratio, land use). As for plants and animals, each habitat hosted generalist and specialist taxa and a specific interaction network directly or indirectly impacted by human activities.Main conclusionsIn a context of global change, our results emphasize that the policies for biodiversity and habitat conservation should now integrate soil microorganisms conceptually and technically.