bluuebunny/crossref_metadata_2025_split huggingface dataset:
Rat epidermal stem cells promote the angiogenesis of full-thickness wound
Authors: Huang Shaobin , Hu Zhicheng , Wang Peng , zhang Yi , Cao Xiaoling , Dong Yunxian , Cheng Pu , Xu Hailin , Zhu Wenkai , Tang Bing , Zhu Jiayuan 
Published: June 2020

Abstract
Background: Full-thickness wounds are a serious problem which badly affects patients’ life quality and also become the difficult problem for clinicians. Stem cells have great prospects in the treatment of wounds. Our previous experiments proved that autologous basal cell suspension can promote wound healing, and there are epidermal stem cells (ESCs) in basal cell suspension. We then conducted experiments to explore the effect of ESCs on full-thickness wound. Methods: In our study, the rat ESCs were isolated and expanded, and transfected with lentivirus to stably express EGFP. Experimental rats were randomly divided into 2 groups, in the ESCs group, the rat ESCs were sprayed on the Full-thickness wounds of the rats, while in control group, sprayed the PBS on the wound. Wound healing and neovascularization were then evaluated. Colonization, division and differentiation of ESCs on the wound were discovered by immunofluorescence.Results: The result suggested that rat ESCs can colonize, divide and proliferate in the wound. What’s more, the rat ESCs around blood vessels can differentiate into vascular endothelial cells and form a lumen-like structure. Compared with the control group, spraying the rat ESCs on the wound bed can promote angiogenesis and accelerate wound healing. Conclusions: Our study proved that rat ESCs were safe and effective for treating full-thickness wounds, and under certain conditions, ESCs can differentiate into vascular endothelial cells to promote angiogenesis and wound healing.
Umbilical cord-derived mesenchymal stromal cells immunomodulate and restore actin dynamics and phagocytosis of LPS-activated microglia via PI3K/Akt/Rho GTPase pathway
Authors: Mukai Takeo , Martino Elena Di , Tsuji Shunichiro , Blomgren Klas , Nagamura-Inoue Tokiko , Ådén Ulrika 
Published: June 2020

Abstract
Background: Microglia are the immune cells in the central nervous system constantly surveying their environment and reacting to various injuries. Activated microglia may cause impaired synaptic plasticity, therefore modulating and restoring them to more neutral phenotype is crucial to counteract a pro-inflammatory, neurotoxic state. In this study, we focused on elucidating whether human umbilical cord (UC) tissue-derived mesenchymal stromal cells (MSCs) can exert immunomodulatory effect and change the phenotype of activated microglia. Methods: Primary culture of microglia was activated by lipopolysaccharide (LPS) and was co-cultured with three lots of MSCs. We investigated immunomodulation, actin dynamics and phagocytic capacity of activated microglia, and examined change of Rho GTPase in microglia as the mechanism. Results: MSCs suppressed the expression of IL-1β and pNFκB in LPS-activated microglia, and conversely elevated the expression of IL-1β in resting-surveying microglia with lot-to-lot variation. Morphological and phagocytotic analyses revealed that LPS stimulation significantly increased active Rho GTPase, Rac1, and Cdc42 levels in the microglia, and their morphology changed to amoeboid in which F-actin spread with ruffle formation. The F-actin spreading persisted after removal of LPS stimulation and reduced phagocytosis. On the other hand, MSC co-culture induced bimodal increase in active Rac1 and Cdc42 levels in LPS-activated microglia. Moreover, extended ruffles of F-actin shrinked and concentrated to form an actin ring, thereby restoring phagocytosis. We confirmed that inhibition of the PI3K/Akt pathway attenuated F-actin dynamics and phagocytosis restored by MSCs. Conclusions: Overall, we demonstrated that MSCs immunomodulated microglia with lot-to-lot variation, and changed the phenotype of LPS-activated microglia, thereby restoring actin dynamics and phagocytosis by increase of active Rho GTPase. Modulation of the phenotype of activated microglia by MSCs can be a novel therapeutic strategy for the treatment of neurological disorders accompanied by inflammation.
Secretome of Adipose-Derived Stem Cells Protect Ischemia-Reperfusion and Partial Hepatectomy by Attenuating Autophagy
Authors: Ma Yajun , Jiao Zhihui , Liu Xiaoning , Zhang Qianzhen , Piao Chenxi , Xu Jiayuan , Wang Hongbin 
Published: June 2020

Abstract
Background: The therapeutic effects of adipose-derived mesenchymal stem cells (ADSCs) may mainly come from their paracrine effects. ADSCs can ameliorate hepatic ischemia-reperfusion injury (IRI). We explored the therapeutic effect of ADSCs secretome from the perspective of excessive autophagy of hepatocytes induced by hepatic IRI.Methods: In this study, we established a miniature pig model of hepatic ischemia-reperfusion (I/R) combined with hepatectomy using laparoscopic technique, and transplanted ADSCs and adipose-derived mesenchymal stem cell-conditioned medium (ADSC-CM) into the liver parenchyma immediately after surgery. Histopathological and TEM examinations were performed on liver tissue samples collected. We analyzed the roles of ADSC-CM and ADSCs in autophagy by RT-qPCR, western-blot and immunohistochemistry. Results: The results showed that ADSCs and ADSC-CM all alleviated the pathological changes of liver tissue and the microstructural damage of hepatocytes after IRI. Moreover, the expression of the critical markers of autophagy including Beclin-1, ATG5, ATG12 and LC3II all decreased, whereas expression of p62 increased. And the data of autophagy regulation between ADSC-CM and ADSCs showed no significant difference. Finally, we found that ADSC-CM possibly inhibited autophagy by regulating the PI3K/Akt/mTOR pathway.Conclusion: ADSC-CM can ameliorate excessive autophagy injury in hepatic I/R combined with partial hepatectomy, which is possibly involved with the modulation of the PI3K/Akt/mTOR signaling pathway. There was no significant difference between ADSCs and ADSC-CM in the regulation of hepatocyte autophagy. Therefore, ADSCs may improve the excessive autophagy injury of hepatocytes in hepatic I/R combined with hepatectomy through paracrine effect, thus protecting the liver and promoting the liver tissue repair.
MYC and TP53 aberrant of CSF tumor cells in children with metastatic medulloblastoma
Authors: Li Miao , Du Shuxu , Li Yujie , Xiao Jiang , Wang Yuan , Zhang Jin , Gong Xiaojun , Sun Yanling , Wu Wanshui , Sun Liming 
Published: June 2020

Abstract
Background: Medulloblastoma (MB) is the most common primary malignant brain tumor in childhood. MYC and TP53 aberrant is the high risk factor for its prognosis, but its values in tumor cells of CSF has not been reported.Methods: During October 1, 2012 to April 10, 2020, a total of 185 metastatic MB were enrolled in this study, CSF cytology and MYC and TP53 aberrant statuses were analyzed.Results: We found that forty-two patients were positive presence of tumor cells in CSF, and 39 cases were positive with MYC or TP53 aberrant, which was present in about 66% of Group 3 MB. The PFS and OS rates of patients with positive gene aberrant were much lower than that of negative aberrant group (P<0.01), and MYC and TP53 aberrant of tumor cells in CSF was high independent risk factor for OS of metastatic patients (95% CI was 0.266-0.998, P<0.05).Conclusion: Thus detection of MYC and TP53 aberrant of tumor cells in CSF could be an important method to complement detection of surgical specimen, and can improve the molecular diagnosis.
The prevalence of uterine rupture and associated factors in Ethiopia: A systematic review and Meta – analysis
Authors: Ayenew Asteray Assmie , Nigussie Azezu Asres , Zewdu Biruk Ferede 
Published: June 2020

Abstract
Background Globally, maternal morbidity and mortality a major public health challenge. Uterine rupture is a life-threatening obstetrical emergency with serious undesired complications for both the mother and her infant. Uterine rupture is the leading cause of maternal and fetal death in developing countries. Therefore, the aim of this systematic review and meta-analysis was to assess the prevalence of uterine rupture and its associated factors in Ethiopia. Method: Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guideline was followed. The databases used were; PubMed, Google scholar, Cochrane Library, African Journals Online, Ethiopian's university research repository online library, and manual searching. The search was further limited to studies conducted in Ethiopia and reported in English. Two authors independently extracted all necessary data using a standardized data extraction format. STATA 11 software was used to analyze the data. The Cochrane Q and I2 test were used to assess the heterogeneity of studies. The pooled estimate prevalence and the odds ratios with 95% confidence intervals were computed by a random effect model. Result: A total of 16 studies were included in this meta-analysis with a sample size of 10,550. The national pooled prevalence of uterine rupture was 14.74%. The pooled adjusted odds ratio (AOR) of uterine rupture for prolonged labour(>24hours) was 4.3 (95% CI=2.57,7.19), not having antenatal care during pregnancy was 3.71(95%CI=2.26,6.08),grand multiparity was 1.99(95%CI=1.04,3.81),rural residency was 4.17 (95%CI=1.72,10.12),having cesarean section scar was 8.52 (95%CI: 4.98,14.59),not using partograph during labour was 3.43(1.62,7.29), and obstructed labour was 8.78(95%CI:3.38,22.8). Conclusion: This systematic review and meta-analysis showed that uterine rupture was high in Ethiopia. Being from the rural residence, prolonged labour(>24hours) , not having ante natal care , grand multiparity, having cesarean section scar , not using partograph during labour, and obstructed labour increased the risk of uterine rupture.
Ion channel profiling of the Lymnaea stagnalis ganglia via transcriptome analysis
Authors: Dong Nan , Bandura Julia , Zhang Zhaolei , Wang Yan , Labadie Karine , Noel Benjamin , Davison Angus , Koene Joris M , Sun Hong-Shuo , Coutellec Marie-Agnes , Feng Zhong-Ping 
Published: June 2020

Abstract
Background. The pond snail Lymnaea stagnalis (L. stagnalis) has been widely used as a model organism in neurobiology, ecotoxicology, and parasitology due to the relative simplicity of its CNS. However, its usefulness is restricted by a limited availability of transcriptome data. While sequence information for the L. stagnalis CNS transcripts has been obtained from EST library and a de novo RNA-seq assembly, the quality of these assemblies is limited by a combination of low coverage of EST libraries, the fragmented nature of de novo assemblies, and lack of reference genome. Results. In this study, taking advantage of the recent availability of the L. stagnalis reference genome, we generated an RNA-seq library from the adult L. stagnalis CNS, using a combination of genome-guided and de novo assembly programs to identify 17,832 protein-coding L. stagnalis transcripts. We combined our library with existing resources to produce a transcript set with greater sequence length, completeness, and diversity than previously available ones. Using our assembly and functional domain analysis, we profiled L. stagnalis CNS transcripts encoding ion channels and ionotropic receptors, which are key proteins for CNS function, and compared their sequences to other vertebrate and invertebrate model organisms. Interestingly, L. stagnalis transcripts encoding numerous putative Ca2+ channels showed the most sequence similarity to those of mouse, zebrafish, Xenopus tropicalis, fruit fly, and C. elegans, suggesting that many calcium channel-related signaling pathways may be evolutionarily conserved. Conclusions. Our study provides the most thorough characterization to date of the L. stagnalis transcriptome and provides insights into differences between vertebrates and invertebrates in CNS transcript diversity, according to function and protein class. Furthermore, this study is, to the best of our knowledge, the first to provide a complete characterization of the ion channels of a single species, opening new avenues for future research on fundamental neurobiological processes.
FOXO3a shRNA and simultaneous induction of P27Kip1gene Inhibit the Breast Cancer Growth in Nude Mice
Authors: Mayahi Sabah , Golalipour Masood , Ashari Mohammad , Shahbazi Majid 
Published: June 2020

Abstract
Background: FOXO proteins, which are overexpressed in multiple human tumors, belong to the Forkhead family of transcription factors that are involved in cell-cycle regulation, cell apoptosis, differentiation, stress response, and metabolism. The p27Kip1 gene leads to cell cycle arrest, cell apoptosis, tumor suppressor genes, and cell adhesion. The low expression level of the p27Kip1 gene is attributed to poor prognosis in patients with colorectal, gastric, pulmonary, and breast cancers. Accordingly, the present study aimed to investigate the possibility of tumor growth inhibition in a mouse model by targeting FOXO3a shRNA and the simultaneous induction of P27Kip1gene.Methods: The tumor model was generated by intratumoral inoculating with plasmids. When tumor size reached an average volume of 8 mm in diameter, the mice received injections of construct and control plasmids three times a week for two weeks, followed by tumor growth assessment.Results: Based on the obtained results, the delivery of construct plasmid significantly inhibited tumor growth in nude mice, as compared to the control plasmid. Moreover, the immunohistochemical analysis indicated that the delivery of construct plasmid significantly suppressed expression of FOXo3a and induced P27Kip1 in tumor samples.Conclusion: The findings of the present study revealed that FOXO3a shRNA, along with simultaneous induction of P27Kip1gene using a useful in vivo gene delivery strategy, seems a practical therapeutic approach for breast cancer treatment and may provide profound insight into gene therapy of solid cancers.
Relationships Between Nursing Management, Nurses’ Job Satisfaction, Patient Satisfaction, and Medication Errors at the unit Level: A Correlational Study
Authors: Nurmeksela Anu , Mikkonen Santtu , Kinnunen Juha , Kvist Tarja 
Published: June 2020

Abstract
Background Nurse managers play a critical role in enhancing nursing and patient outcomes. Nurse managers’ behaviour and the clinical environment have been shown to affect nurses’ job satisfaction. In addition, leadership, managers’ support, and nurse-manager trust all improve the patient safety culture climate and reduce medication errors. However, hospital management and organisational support influence both manager and staff attitudes, which can affect care quality outcomes. This study aims to describe the relationships between nurse managers’ work content, nurses’ job satisfaction, patient satisfaction, and medication errors at the hospital unit level. Methods The questionnaire data were collected from nurse managers (n= 29), nursing staff (n=306), and patients (n=651) from 28 units across three Finnish acute care hospitals in year 2017. Analysis of covariance (ANCOVA) was used to estimate relationships between data from Nurse Managers’ Work Content Questionnaire, Kuopio University Hospital Job Satisfaction Scale, and Revised Humane Caring Scale subscales, along with medication errors reports. Results Multiple relationships were identified for components of nurse managers’ work content, nurses’ job satisfaction, patient satisfaction, and medication errors. How nurse managers divide their time between various responsibilities was shown to influence the patient satisfaction Outcomes subarea (p< .001), the Requiring factors of work subarea of nurses’ job satisfaction (p< .001), as well as medication errors (p< .001).Conclusions It would be recommendable for nurse managers to prioritise focusing on employee well-being, development, and communication. However, to be effective and beneficial, the work in each of these subareas must be well organised. The results demonstrate that nurse managers need to consciously balance their workloads to achieve better outcomes.
Comparison of levonorgestrel level and creamatocrit in milk following immediate vs. delayed postpartum insertion of levonorgestrel IUD: a pilot randomized controlled trial
Authors: Hopelian Niaree , Simmons Rebecca G. , Sanders Jessica N. , Ward Katherine , Jenkins Sabrina Malone , Espey Eve , Turok David K. 
Published: June 2020

Abstract
Background: Both breastfeeding and the use of postpartum contraception critically influence infant and maternal health outcomes. In this pilot study, we explore the effects of timing and duration of postpartum levonorgestrel exposure on breastfeeding through objective assessments of milk lipid and levonorgestrel content to establish baseline data for future research.Methods: This substudy includes thirty-two participants from multiple clinical sites in Salt Lake City, Utah. All participants planned to breastfeed, and self-selected for this sub-study from among 259 women enrolled in a parent randomized controlled trial comparing immediate to delayed (4-8 weeks) postpartum levonorgestrel IUD insertion. Participants provided two milk samples: first at 4-8 weeks postpartum (before IUD placement for the delayed group) and second four weeks later. Participants were paired by group assignment, and we used the Wilcoxon rank sum (inter-group) and signed rank (intra-group) tests to compare milk lipid content (creamatocrit) and levonorgestrel levels between groups and time points.Results: Fifteen participants were allocated to the immediate group and 17 to the delayed group. Initially, median levonorgestrel concentration of the immediate group (n=10) (32.5 pg/mL, IQR: 24.8, 59.4) exceeded that of the delayed group (n=12) (17.5 pg/mL, IQR: 0.0, 25.8) (p=0.01). Four weeks later, the values aligned: 26.2 pg/mL (IQR: 20.3, 37.3) vs. 28.0 pg/mL (IQR: 25.2, 40.8). Creamatocrits were similar between both groups and timepoints.Conclusions: Immediate postpartum levonorgestrel IUD placement results in steady, low levels of levonorgestrel in milk without significant effects on lipid content. These findings support the safety of immediate postpartum levonorgestrel IUD initiation, and future research should assess time-based meaningful outcome changes to capture clinically significant differences.Trial Registration: This randomized controlled trial was registered with ClinicalTrials.gov (registry number NCT01990703) on November 21, 2013.
Metatranscriptomic analyses reveal ruminal pH regulates fiber degradation and fermentation by shifting the microbial community and gene expression of carbohydrate-active enzymes
Authors: Li MengMeng , White Robin R. , Guan Le Luo , Harthan Laura , Hanigan Mark D. 
Published: June 2020

Abstract
Background
Volatile fatty acids (VFA) generated from ruminal fermentation by microorganisms provide up to 75% of total metabolizable energy in ruminants. Ruminal pH is an important factor affecting the profile and production of VFA by shifting the microbial community. However, how ruminal pH affects the microbial community and its relationship with expression of genes encoding carbohydrate-active enzyme (CAZyme) for fiber degradation and fermentation are not well investigated. To fill in this knowledge gap, six cannulated Holstein heifers were subjected to a continuous 10-day intraruminal infusion of distilled water or a dilute blend of hydrochloric and phosphoric acids to achieve a pH reduction of 0.5 units in a cross-over design. RNA-seq based transcriptome profiling was performed using total RNA extracted from ruminal liquid and solid fractions collected on day 9 of each period, respectively.
Results
Metatranscriptomic analyses identified 19 bacterial phyla with 121 genera, 3 archaeal genera, 11 protozoal genera, and 97 CAZyme transcripts in sampled ruminal contents. Within these, 4 bacteria phyla (Proteobacteria, Firmicutes, Bacteroidetes, and Spirochaetes), 2 archaeal genera (Candidatus Methanomethylophilus and Methanobrevibacter), and 5 protozoal genera (Entodinium, Polyplastron, Isotricha, Eudiplodinium, and Eremoplastron) were considered as the core active microbes, and genes encoding for cellulase, endo-1,4-beta- xylanase, amylase, and alpha-N-arabinofuranosidase were the most abundant CAZyme transcripts distributed in the rumen. Rumen microbiota is not equally distributed throughout the liquid and solid phases of rumen contents, and ruminal pH significantly affect microbial ecosystem, especially for the liquid fraction. In general, 76 bacterial genera, 4 protozoal genera, and 48 genes encoding CAZyme were significantly correlated with metabolic pathways for fiber degradation and VFA production. Within them, 29 bacterial genera, 4 protozoal genera, and 6 genes encoding CAZyme could be regulated by ruminal pH.
Conclusions
The ruminal microbiome changed the expression of transcripts for biochemical pathways of fiber degradation and VFA production in response to reduced pH, and at least a portion of the shifts in enzyme transcripts was associated with altered microbial community structure.
Early Axonal Dysfunction of the Peripheral Nervous System Influences Disease Progression of ALS: Evidences From Clinical Neuroelectrophysiology
Authors: Yu Huiyan , Chen Lu , Zhang Shuo , He Jing , Fan Dongsheng 
Published: June 2020

Abstract
Background:Studies have indicated that more axonal dysfunction in early ALS predictive of more aggressive phenotypes. However, as an important electrophysiological index to detect motor axonal damage, the prognostic value of compound muscle action potential (CMAP) in ALS remains unclear. We aimed to determine if CMAP could be a prognostic indicator of disease progression in early ALS.Methods: Patients were stratified into 4 groups according to the decrement of the CMAP amplitude within 12 months of symptom onset (CMAP-12 amplitudes): normal (≥the lower limit of normal, LLN), mild (<LLN but ≥50% of LLN), moderate (<50% but ≥30% of LLN) and severe (<30% of LLN). All patients were followed up every 3 months. Survival was analyzed using the Kaplan-Meier method and Cox proportional hazards regression.Results: A total of 149 patients included in the analysis [90 male (60.4%); mean age at onset, 50.7 years]. The decrement of CMAP-12 amplitudes was normal in 24.2%, mildly in 22.1%, moderately in 15.4% and severely in 38.3%. Kaplan-Meier analysis showed there was a significant difference in the overall survival across the 4 groups (p<0.05). Further pairwise comparison found there were significant differences in survival between the normal vs. the moderately groups (p<0.05), and the normal vs. the severely groups (p<0.01). There was a significant inverse correlation between the CMAP-12 amplitude and overall survival. Compared to the normal group, survival in the moderately and severely decreased groups were significantly shorter (HR 3.394, 95%CI 1.292-8.917, p=0.013; and HR 4.732, 95%CI 2.032-11.017; p=0.000, respectively).Conclusions: Our results suggested that CMAP-12 amplitude could be a prognostic indicator of disease progression in ALS. More importantly, it provided clinical evidences to the viewpoint that early axonal dysfunction of peripheral nervous system accelerates disease progression of ALS.