bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pervasive Pesticide Contamination of Wetlands in the Great Barrier Reef Catchment Area
Authors: Vandergragt Maria L , Warne Michael St J , Borschmann Geoffrey , Johns Caitlin V 
Published: June 2020

Abstract
Knowledge of the types and impacts of contaminants occurring in the freshwater wetlands of the Great Barrier Reef catchment area (GBRCA) is limited. The present study examined the presence and concentrations of pesticides occurring in 22 floodplain wetlands, situated in moderate to high-intensity land uses in the GBRCA. The dominant land use within 1 km of the wetlands was sugar cane for 12 wetlands, grazing for 6 wetlands, plantation forestry and conservation for 2 wetlands, and one with an equal mix of land uses. Fifty-nine pesticides and pesticide degradates were detected in the wetlands during 2 consecutive early wet seasons. These included 27 herbicides, 11 herbicide degradates, 11 insecticides, 8 fungicides, 1 nematicide, and 1 pesticide synergist. Each wetland sampled contained between 12 and 30 pesticides with an average of 21 pesticides detected per wetland sampling. Temporal differences existed in the number, types, and average concentrations of pesticides detected. No exceedances of Australian and New Zealand water guideline values were found during the first sampling season, while 10 wetlands had concentrations of at least 1 pesticide exceeding the guidelines during the following sampling season. For 1 wetland, concentrations of 4 pesticides were greater than the prescribed guideline values. Individually, the vast majority of aquatic species would be protected, but in some wetlands, diuron would affect 49% of species and atrazine up to 24% of species. Statistically significant correlations between the number of pesticides and the percentage of intensive land use, primarily sugar cane growing in a 1 km radius of the wetlands, were found. Integr Environ Assess Manag 2020;16:968–982. © 2020 SETAC
Attachment & Child Health (ATTACH) pilot trials: Effect of parental reflective function intervention for families affected by toxic stress
Authors: Letourneau Nicole , Anis Lubna , Ntanda Henry , Novick Jason , Steele Miriam , Steele Howard , Hart Martha 
Published: June 2020

ABSTRACTToxic stressors (e.g., parental violence, depression, low income) place children at risk for insecure attachment. Parental reflective function—parents’ capacity to understand their own and their child's mental states and thus regulate their own feelings and behavior toward their child—may buffer the negative effects of toxic stress on attachment. Our objective was to test the effectiveness of the Attachment and Child Health (ATTACH) intervention, focusing on improving reflective function and children's attachment security, for at‐risk mothers and children <36 months of age. Three pilot studies were conducted with women and children from an inner city agency serving vulnerable, low‐income families and a family violence shelter. Randomized control trial (n = 20, n = 10 at enrollment) and quasi‐experimental (n = 10 at enrollment) methods tested the effect of the ATTACH intervention on the primary outcome of reflective function scores, from transcribed Parent Development Interviews. Our secondary outcome was children's attachment patterns from Ainsworth's Strange Situation Procedure. Despite some attrition, mixed methods analysis of covariance and t tests revealed significant differences in maternal, child, and overall reflective function, with moderate effect sizes. While more children whose mothers received the ATTACH program were securely attached posttreatment, as compared with controls, significant differences were not observed, which may be due to missing observations (n = 5 cases). Understanding the effectiveness of programs like the ATTACH intervention contributes to improved programs and services to promote healthy development of children affected by toxic stress.
Average treatment effects for stayers with correlated random coefficient models of panel data
Authors: Verdier Valentin 
Published: June 2020

SummaryCorrelated random coefficient (CRC) models provide a useful framework for estimating average treatment effects (ATE) with panel data by accommodating heterogeneous treatment effects and flexible patterns of selection. In their simplest form, they lead to the well‐known difference‐in‐differences estimator. CRC models yield estimates of ATE for “movers” (i.e., cross‐sectional units whose treatment status changed over time) while ATE for “stayers” (i.e., cross‐sectional units who retained the same treatment status over time) are not identified. We study additional restrictions on selection into treatment that lead to the identification of ATE for stayers by an extrapolation from quantities identified by the CRC model. We discuss estimation and testing of the extrapolation's validity, then use our results to estimate the returns to agricultural technology adoption among maize farmers in Kenya.
Structural phase formation and in vitro bioactivity evaluations of strontium phosphosilicate for orthopedic applications
Authors: Chetan  , Vijayalakshmi Uthirapathy 
Published: June 2020

AbstractStructural features of apatites make them one of the most promising candidates for bone tissue regenerative applications. The unique structure and availability of mobile Metal ion as well as other components help interaction with biological fluids and can promote as well as stimulate bone regeneration with correct components. The present study focusses on Strontium phosphosilicate, an apatite analogue to Calcium phosphate‐based HAP only loaded with better composition replacing Calcium with stimulatory Strontium and co‐existent Silicate alongside phosphate both known to stimulate osteogenesis. Bulk particles were synthesized as powders with Acidic medium as well as the Basic medium of reaction mixture via Sol–Gel and Co‐precipitation techniques respectively and phase formation was studied with respect to temperature further detailed by TGA‐DSC studies. Secondary phases were also indexed based on which Acidic medium samples sintered at 800°C were comparatively better from the Basic medium on account of the presence of silicate phase forming agglomerated Strontium phosphosilicate. Hemolysis assay and MG‐63 based cytotoxicity assay were carried out to study biocompatibility and antibacterial properties were also elucidated in Gram‐positive and Gram‐negative bacteria. Apatite seeding and bone mineralization studies were carried out with Simulated body fluid and characterized structurally and morphologically.
MAGI2‐AS3 rs7783388 polymorphism contributes to colorectal cancer risk through altering the binding affinity of the transcription factor GR to the MAGI2‐AS3 promoter
Authors: Yang Xi , Wu Shenshen , Li Xiaobo , Yin Ying , Chen Rui 
Published: June 2020

AbstractBackgroundIt has been indicated that the single nuclear polymorphisms (SNPs) in the long noncoding RNA (lncRNA) have association with colorectal cancer (CRC) susceptibility.MethodsWe enrolled 1078 cases with CRC and 1175 age‐ and gender‐matched cancer‐free controls to explore whether the polymorphisms in MAGI2‐AS3 have associations with CRC risk. qRT‐PCR, expression quantitative trait loci (eQTL) analyses, dual‐luciferase reporter assay, chromatin immunoprecipitation (ChIP), flow cytometry, and transwell assays were performed to explore the specific mechanisms in which MAGI2‐AS3 rs7783388 variation influenced the tumorigenesis of CRC.ResultsSubjects carrying rs7783388 GG genotype presented a higher risk of CRC compared with the AG/AA genotypes. Mechanistically, we found that the functional genetic variant of rs7783388 A > G decreased binding affinity of transcription factor glucocorticoid receptor (GR) to the MAGI2‐AS3 promoter, resulting in decreased transcriptional activity that subsequently downregulated MAGI2‐AS3 expression. Furthermore, functional experiments elucidated that MAGI2‐AS3 overexpression suppressed CRC cell proliferation, migration, and invasion capacities, arrested cell cycle at G0/G1 phase, and promoted cell apoptosis.ConclusionTaken together, our study demonstrated that the potential function of MAGI2‐AS3 as a tumor suppressor for CRC, and the MAGI2‐AS3 rs7783388 polymorphism is associated with the increased susceptibility to CRC by altering the binding ability of GR to the MAGI2‐AS3 promoter.
CCL20 is a novel ligand for the scavenging atypical chemokine receptor 4
Authors: Matti Christoph , D'Uonnolo Giulia , Artinger Marc , Melgrati Serena , Salnikov Angela , Thelen Sylvia , Purvanov Vladimir , Strobel Tobias D. , Spannagel Lisa , Thelen Marcus , Legler Daniel F. 
Published: June 2020

Abstract
The chemokine CCL20 is broadly produced by endothelial cells in the liver, the lung, in lymph nodes and mucosal lymphoid tissues, and recruits CCR6 expressing leukocytes, particularly dendritic cells, mature B cells, and subpopulations of T cells. How CCL20 is systemically scavenged is currently unknown. Here, we identify that fluorescently labeled human and mouse CCL20 are efficiently taken-up by the atypical chemokine receptor ACKR4. CCL20 shares ACKR4 with the homeostatic chemokines CCL19, CCL21, and CCL25, although with a lower affinity. We demonstrate that all 4 human chemokines recruit β-arrestin1 and β-arrestin2 to human ACKR4. Similarly, mouse CCL19, CCL21, and CCL25 equally activate the human receptor. Interestingly, at the same chemokine concentration, mouse CCL20 did not recruit β-arrestins to human ACKR4. Further cross-species analysis suggests that human ACKR4 preferentially takes-up human CCL20, whereas mouse ACKR4 similarly internalizes mouse and human CCL20. Furthermore, we engineered a fluorescently labeled chimeric chemokine consisting of the N-terminus of mouse CCL25 and the body of mouse CCL19, termed CCL25_19, which interacts with and is taken-up by human and mouse ACKR4.
Clinical characteristics of COVID‐19 in patients with preexisting ILD: A retrospective study in a single center in Wuhan, China
Authors: Huang Hong , Zhang Ming , Chen Can , Zhang Huilan , Wei Yanqiu , Tian Jianbo , Shang Jin , Deng Yan , Du Aihua , Dai Huaping 
Published: June 2020

AbstractSince the outbreak of 2019 novel coronavirus (SARS‐CoV‐2) pneumonia, many patients with underlying disease, such as interstitial lung disease (ILD), were admitted to Tongji hospital in Wuhan, China. To date, no data have ever been reported to reflect the clinical features of Corona Virus Disease 2019 (COVID‐19) among these patients with preexisting ILD. We analyzed the incidence and severity of COVID‐19 patients with ILD among 3201 COVID‐19 inpatients, and compared two independent cohorts of COVID‐19 patients with pre‐existing ILD (n = 28) and non‐ILD COVID‐19 patients (n = 130). Among those 3201 COVID‐19 inpatients, 28 of whom were COVID‐19 with ILD (0.88%). Fever was the predominant symptom both in COVID‐19 with ILD (81.54%) and non‐ILD COVID‐19 patients (72.22%). However, COVID‐19 patients with ILD were more likely to have cough, sputum, fatigue, dyspnea, and diarrhea. A very significantly higher number of neutrophils, monocytes, interleukin (IL)‐8, IL‐10, IL‐1β, and D‐Dimer was characterized in COVID‐19 with ILD as compared to those of non‐ILD COVID‐19 patients. Furthermore, logistic regression models showed neutrophils counts, proinflammatory cytokines (tumor necrosis factor‐alpha, IL6, IL1β, IL2R), and coagulation dysfunction biomarkers (D‐Dimer, PT, Fbg) were significantly associated with the poor clinical outcomes of COVID‐19. ILD patients could be less vulnerable to SARS‐CoV‐2. However, ILD patients tend to severity condition after being infected with SARS‐CoV‐2. The prognosis of COVID‐19 patients with per‐existing ILD is significantly worse than that of non‐ILD patients. And more, aggravated inflammatory responses and coagulation dysfunction appear to be the critical mechanisms in the COVID‐19 patients with ILD.
Impact of climate change on inter‐annual variation in tea plant output in Zhejiang, China
Authors: Lou Weiping , Sun Ke , Zhao Yuming , Deng Shengrong , Zhou Zhuding 
Published: June 2020

AbstractRecent studies have shown that climate change is having severe impacts on production of the tea plant [Camellia sinensis (L.) Kuntze], an important woody cash crop. Quantitative production figures are necessary to provide tea stakeholders and policy makers with evidence to justify immediate action. We used data from Zhejiang Province, China, and the economic output model of four tea plant cultivars (Jiaming 1, Longjing 43, Baiye 1, and Jiukeng) in spring and the yield output model of one tea plant cultivar (Jiukeng) in summer and autumn, and analyzed the impact of climate change on tea production from 1985 to 2018. The results showed that the effects of high temperature and drought on tea production have increased significantly. Temperature was the main factor effecting the economic output of tea plants in spring. Climate change has resulted in the start date of tea buds and leaves plucking period to have become significantly earlier in spring. The risk of frost damage and economic loss, caused by frost, decreased significantly in the period of spring tea production. Among the five bud and leaf grades, the length of plucking periods for superfine, Grades 1 and 2 decreased significantly. High temperatures and drought were the main factors impacting tea production in summer and autumn, and effected the economic output of spring tea in the following year. The economic losses caused by daytime rainfall decreased significantly in spring tea production. This study has provided essential evidence that climate change has already had a significant impact on tea plant output.
Leading modes of Asian–Australian monsoon interannual variability as represented in CMIP5 models
Authors: Zhou Fang , Ren Hong‐Li , Liu Ming‐Hong , Wang Run , Huang Kai 
Published: June 2020

AbstractThe Asian–Australian monsoon (AAM) exerts a strong impact on the regional climate. The ability of climate models to simulate the AAM is of great importance. This study evaluates the performance of 26 models, which participate in the Coupled Model Intercomparison Project phase 5 (CMIP5), in representing features of the leading modes of the AAM interannual variability. This is achieved by diagnosing quantitative metrics derived from a Season‐reliant Empirical Orthogonal Function (S‐EOF) analysis, and plausible external forcing contributing to the simulation results are presented. Our results show that the CMIP5 models can generally capture the spatial patterns of the leading modes of AAM interannual variability during El Niño development periods because of strong air–sea interactions. The variance percentage explained by the first two S‐EOF modes is found to be closely related to the amplitude of the El Niño‐Southern Oscillation (ENSO) in the models. In contrast, simulation of the periodicity remains challenging, and the models show deficiencies in the accurate capture of periodic characteristics. Models with realistic ENSO simulations typically perform well in simulating S‐EOF1, and the key to improving the simulation of S‐EOF2 is the greater intensity of the simulated field of background heating over the Northwest Pacific warm pool. The multimodel ensemble (MME) performs better than most of the 26 individual models. Models in the MME with better ENSO simulation and stronger warm pool heating are found to be better able to represent the relationships between the first two leading S‐EOF modes and ENSO, although biases remained.
A quantitative serum biomarker of circulating collagen X effectively correlates with endochondral fracture healing
Authors: Working Zachary M. , Morris Elizabeth R. , Chang Jiun Chiun , Coghlan Ryan F. , Johnstone Brian , Miclau Theodore , Horton William A. , Bahney Chelsea S. 
Published: June 2020

AbstractCurrently, there are no standardized methods for quantitatively measuring fracture repair. Physicians rely on subjective physical examinations and qualitative evaluation of radiographs to detect mineralized tissue. Since most fractures heal indirectly through a cartilage intermediate, these tools are limited in their diagnostic utility of early repair. Prior to converting to the bone, cartilage undergoes hypertrophic maturation, characterized by the deposition of a provisional collagen X matrix. The objective of this study was to characterize the kinetics of a novel collagen X biomarker relative to other biological measurements of fracture healing using a murine model of endochondral fracture repair in which a closed, mid‐shaft tibia fracture was created using the classic drop‐weight technique. Serum was collected 5 to 42 days post‐fracture in male and female mice and compared to uninjured controls (n = 8‐12). Collagen X in the serum was quantified using a recently validated ELISA‐based bioassay (“Cxm”)1 and compared to genetic and histological markers of fracture healing and inflammation. We found the Cxm biomarker reliably increased from baseline to a statistically unique peak 14 days post‐fracture that then resolved to pre‐fracture levels by 3 weeks following injury. The shape and timing of the Cxm curve followed the genetic and histological expression of collagen X but did not show a strong correlation with local inflammatory states. Assessment of fracture healing progress is crucial to making correct and timely clinical decisions for patients. This Cxm bioassay represents a minimally invasive, inexpensive technique that could provide reliable information on the biology of the fracture to significantly improve clinical care.
Preparation and textural properties of white salted noodles made with hard red winter wheat flour partially replaced by different levels of cross‐linked phosphorylated RS4 wheat starch
Authors: Hsieh Chao‐Feng , Wang Li‐Kun , Xu Bin , Seib Paul A , Shi Yong‐Cheng 
Published: June 2020

AbstractBACKGROUNDResistant starch (RS) has health benefits and can be used as a functional ingredient in various food products. Kansas hard red winter (HRW) wheat is conventionally used for bread making and this is attributed to its strong gluten network. To develop Asian white salted noodles with a high RS content, HRW wheat flour was partially replaced with cross‐linked phosphorylated RS4 wheat starch. Vital wheat gluten or wheat protein isolate was added to compensate for textural changes due to the addition of RS.RESULTSThe maximum recommended level of RS4 starch to replace HRW wheat flour was 40%. The substitution of 10–40 parts of RS4 for flour did not change hardness in cooked noodles but it did reduce their extensibility, cohesiveness, and springiness, which was probably due to the non‐swelling properties of RS4. At 40 parts of RS4 replacement, supplementation of 2–8 parts of vital wheat gluten or wheat protein isolate in the composite flour notably enhanced the hardness and extensibility of cooked noodles, whereas cohesiveness and springiness were minimally affected. Supplemental vital wheat gluten produced a thicker protein network than endogenous protein or added wheat protein isolate, giving cooked noodles greater breaking force and distance.CONCLUSIONRS4 could be used as a functional ingredient to replace up to 40% of hard wheat flour for making Asian noodles while maintaining their hardness after cooking. The extensibility of cooked noodles with high RS4 could be noticeably enhanced by supplementation with vital wheat gluten in the composite flour (RS/flour = 40/60). © 2020 Society of Chemical Industry
Effects of soluble starch synthase IIa allelic variation on rice grain quality with different Waxy backgrounds
Authors: You Hui , Zhang Ouling , Xu Liang , Liang Cheng , Xiang Xunchao 
Published: June 2020

AbstractBACKGROUNDSoluble starch synthase IIa (ALK, SSII‐3) is the major gene regulating gelatinization temperature (GT) and SSII‐3 M1 is an effective marker for identifying SSIIa alleles. However, the haplotypes of SSIIa alleles amplified by SSII‐3M1 and their allelic effect sunder different Waxy (Wx) background in non‐glutinous rice remain unclear.RESULTSBy integrating the genetic background analysis and by the identification of the genotypes of Wx and SSIIa, we found that the SSIIa alleles amplified by SSII‐3 M1 were haplotype 1 (G/G/GC, indica‐type) and 4 (A/G/TT, japonica‐type), which had a significant effect on pasting temperature (PaT), hot paste viscosity (HPV), and the alkali spreading value (ASV). There were significant effects of SSIIa alleles on HPV, cool paste viscosity (CPV), and consistency value with different Wx backgrounds. The apparent amylose content (AAC) of samples significantly affected the accuracy of GT, which was represented by the manually determined pasting temperature (PTm).CONCLUSIONSThe SSIIa alleles amplified by SSII‐3 M1 are indica type and japonica type. Different SSIIa haplotypes significantly affect HPV, CPV, PaT, and ASV. GT, PaT, and PTm are mainly affected by SSIIa alleles. The classification of all samples with different haplotypes of SSIIa indicates that their AAC (Wx genotypes) is essentially. The effects of SSIIa alleles are themselves affected by different Wx alleles. © 2020 Society of Chemical Industry
Extensive Use of Intertidal Habitat by Shorebirds Outside Protected Nesting Areas
Authors: Monk Julia D. , Kwon Eunbi , Derose‐Wilson Audrey , Robinson Samantha G. , Bellman Henrietta A. , Karpanty Sarah M. , Catlin Daniel H. , Fraser James D. 
Published: June 2020

ABSTRACTConservation of shorebirds throughout their breeding and migratory ranges has become a priority as shorebird populations decline globally. Along the North Atlantic Coast, management efforts have particularly focused on preserving nesting habitat for piping plovers (Charadrius melodus), which are protected under the Endangered Species Act. It is unclear whether these conservation measures suffice to protect foraging habitat for piping plovers and other shorebirds on stopover during migration along the Atlantic Flyway. To evaluate the extent to which conservation of piping plover nesting areas extends to all habitats used by plovers, and to determine whether these protections also benefited non‐breeding migratory shorebirds in the region, we conducted weekly shorebird surveys, recording the number and locations of piping plovers and other species, during northward and southward migration on Fire Island and Westhampton Island, New York, USA, from 2014–2016. We used canonical correspondence analysis (CCA) to assess the degree of spatiotemporal overlap between breeding plovers, foraging plovers, and other migratory shorebirds that temporarily stage at the site. The spatiotemporal distribution of migratory shorebirds matched more closely with piping plovers seen during foraging than piping plovers observed tending nests and engaging in other breeding activities. Migratory shorebirds and foraging piping plovers were more abundant and frequent in wet intertidal zones outside of fenced‐off nesting areas, which were not protected under current management regimes. Therefore, additional protection of piping plover foraging habitat could benefit plovers and migratory shorebirds that use similar feeding grounds during stopover on northward and southward migration. © 2020 The Wildlife Society.