bluuebunny/crossref_metadata_2025_split huggingface dataset:
Percutaneous left atrial appendage occlusion in nonvalvular atrial fibrillation patients with coronary heart disease
Authors: Zhang Juan , Jiang Xiaomin , Xie Dujiang , Luo Jie , Zhou Ling 
Published: March 2020

Abstract
Purpose To evaluate the 12-month outcomes of the percutaneous left atrial appendage occlusion (LAAO) procedure in nonvalvular atrial fibrillation (NVAF) patients with coronary heart disease (CAD). Materials and Methods 51 NVAF patients were consecutively accepted LAAO between June 2015 and July 2017. Patients were devided in two groups: 15 with CAD and 36 without CAD. All patients were followed up at 1st, 3rd, 6th, and 12th month after discharge with transesophageal echocardiography (TEE) examination repeated at the same time. Results Among these 51 patients (average age 64.4±10.0, 33.3% female), the procedural success rate was 98% (50/51). During 12-month follow-up, there were no hemorrhagic stroke or major bleeding. The risk of thromboembolism based on CHA2DS2-VASc score (3.1±1.6 vs. 5.2±1.1, P<0.001) and the risk of hemorrhage based on HAS-BLED score (2.2±1.2 vs. 3.1±1.0, P=0.009) in CAD group were significantly higher. The incidence of end-point events had no statistical difference between CAD and non-CAD. Compared with CAD patients who accepted long-term antithrombotic medication, there was no obvious difference in stroke rate and mortality in CAD accepted LAAO group, whereas a further reduction of hemorrhage (n=5/20% vs. n=0, P=0.039) was shown. A significant correlation (P<0.001, r=0.580) was detected between moderate or severe left atrial spontaneous echo contrast (LASEC) and the composite end point events. Conclusion There are similar safety and effectiveness for LAAO procedure in NVAF patients with CAD and without CAD under new oral anticoagulants applied post-implantation anticoagulation strategy. Meanwhile, LASEC is a predictive factor of LAAO in NVAF patients combined CAD.
DLBCL with amplification of JAK2/PD-L2 exhibits PMBCL-like CNA pattern and worse clinical outcome resembling those with MYD88 L265P mutation
Authors: Xue Xuemin , Huang Wenting , Qiu Tian , Guo Lei , Ying Jianming , Lv Ning 
Published: March 2020

Abstract
Background: Recently, copy number alteration (CNA) of 9p24.1 were demonstrated in 10% of diffuse large b-cell lymphoma (DLBCL), with gene expression and mutation profiles that were similar to those of primary mediastinal large B-cell lymphoma(PMBCL). However, their CNA-based profile and clinical impact still remain unclear. Methods: Multiplex ligation-dependent probe amplification were employed to investigate the prevalence of JAK2/PD-L2 amplification in DLBCL and their CNA-based pattern of driver genes. The clinical outcome and characteristics were also analyzed. Results: Using unsupervised hierarchical clustering, a small group of DLBCL (11.7%, 9/77) was clustered together with PMBCL as Cluster_2, demonstrating amplification of JAK2 (100%,9/9) and PD-L2 (77.8%,7/9). This subgroups of DLBCL demonstrated significant higher expression of PD-L1 than those with MYD88 L265P mutation(p=0.011). And they exhibited dismal OS and PFS as compared with DLBCL_others(p=0.021 and 0.015, respectively), which is similar to DLBCL with MYD88 L265P mutation. Conclusions: DLBCL with amplification of JAK2/PD-L2 exhibits CNA pattern that is similar to PMBCL, and worse clinical outcome that resembles those with MYD88 L265P mutation.
Alteration of gut microbiota in elderly postmenopausal women with osteoporosis
Authors: Li Yingjuan , Mu Guangjian , Ma Binbin , Lu Panpan , Huang Haiquan , Ren Liqun , Chen Hui , Rui Yunfeng , Zou Jihong , Liu Naifeng 
Published: March 2020

Abstract
Osteoporosis is a common bone disorder worldwide and causes bone fragility and fracture. Gut microbiota colonizes the gastrointestinal tract, and is associated with bone metabolism and osteoporosis. In our study, the alteration of gut microbiota in osteoporosis and its effects on bone metabolism were investigated. A total of 36 elderly postmenopausal osteoporotic women and 12 healthy controls were recruited, and their fecal samples were collected for 16S rRNA gene sequencing of gut microbiota on Illumina MiSeq platform. The venous blood and urine samples were also collected to determine the biochemical indexes. There was no obvious difference in Alpha diversity in the experiment group and control group, while differential Beta diversity was observed. The Partial Least Squares Discriminant Analysis (PLS-DA) model and variable importance in projection (VIP) scores showed that the osteoporotic women and healthy controls had different genera of Erysipelotrichaceae , Rothia , and Eubacterium . The metabolic function prediction of gut microbiota indicated that the experiment group had 634 unique functional categories, while the control group had 13 unique functional categories. The biochemical measurement revealed that the osteoclast activity indexes including urine N-terminal telopeptides of type I collagen (NTX), serum NTX, and serum C-terminal telopeptides of type I collagen (CTX) in the experiment group were higher than those in the control group, indicating that the osteoclast activity in osteoporotic women was increased. In addition, the correlation analysis of microbial metabolism with phenotypes showed the pathways in the significant modules of magenta, red, pink, and yellow were positively correlated with urine phosphate, urine creatinine, urine creatinine, serum calcium and other biochemical indexes. Collectively, our study identified the different genera between postmenopausal osteoporotic women and healthy controls, which might be potential targets for the treatment of osteoporosis.
Mining biomarkers for the occurrence, development and therapeutic response to infliximab of rheumatoid arthritis based on weighted gene co-expression network analysis
Authors: Wang Jian , Zhang Na , Chen Di , Fu Qiang , Wang Zaijun , Zhao Hui 
Published: March 2020

Abstract
Rheumatoid arthritis (RA) is a chronic systemic autoimmune and recurrent disease characterized by joint lesion with high disability rate and poor prognosis. Although anti-TNF therapy (infliximab) has been validated to be effective in controlling the symptoms of RA, more than one-third of patients cannot benefit from anti-TNF therapy. Therefore, it is critical to explore novel biomarkers associated with the occurrence and development of the disease, and therapeutic response of infliximab treatment. In this study, we first analyzed differentially expressed genes (DEGs) between RA patients and healthy controls. Subsequently, WGCNA was used to analyze the co-expression module (tan module) associated with the therapeutic response of infliximab treatment in RA, which further found that the genes (a total of 102) in the module tan were also the DEGs between RA patients and healthy controls (confirming that the close correlation of these genes with the occurrence and development of RA). Subsequently, enrichment analysis revealed that these 102 genes were mainly related to the body’s inflammatory immune response and the functions of NOD-like receptor signaling pathway as well as miR-146a-5p. Moreover, protein-protein interaction (PPI) was constructed on the 102 genes, followed by mining the key genes in the network (a total of 14 genes) by topological analysis. Finally, we confirmed that 10 genes (OAS1, STAT1, IFIT3, IFIT2, IFI6, RSAD2, OASL, GBP2, IRF7 and IFIT1) were potential biomarkers for the occurrence and development, as well as the therapeutic response against infliximab treatment in RA by analyzing the differences in the expressions of these genes between RA and healthy control samples, as well as drug response and non-response samples. Therefore, this study can provide guidance on medication selection and prognostic prediction of RA patients for clinicians, which can also identify relevant genes to provide therapeutic targets for precise medicine.
Identification of Stage-associated MicroRNAs in Lung Adenocarcinoma Based on Microarray Data
Authors: Yu Jiarui , Wang Wei , Gao Peng , Chen Siyuan , Liu Meiyue , Gu Jinling , Chen Xin , Sun Guogui 
Published: March 2020

Abstract
Background: Lung adenocarcinoma (LUAD) is the most aggressive and most frequently seen histological variant of lung cancers, comprising nearly 45% of the overall cases of lung carcinoma and its incidence has been increased significantly worldwide in the last several decades. However, there was limited available evidence with respect to the signalling pathways and miRNAs related to the LUAD.Methods: To identify the differentially-expressed miRNAs (DE-miRNAs) in different stages of LUAD, we performed a microarray on 514 LUAD and 54 normal tissues. At the mean time, the potentially targeted genes as well as the highly-enriched signaling pathways and the relevant protein–protein interaction (PPI) network were analyzed and constructed by using a series of bioinformatic methods. Moreover, the identified DE-miRNAs in different stages of the LUAD were verified by using the TCGA dataset.Results : Overall 41 down-regulated -and 82 up-regulated DE-miRNAs were identified, of which 1,716 potential targeted genes were selected. Moreover, the enriched pathways of the above genes were screened by using the GO term and KEGG pathway analyses, including the AMPK signalling pathway, FoxO signalling pathway, MAPK signalling pathway, PI3K-Akt signalling pathway and hippo signalling pathway.Conclusions: Our study could provide additional understanding of the underlying molecular events and increase the precision of prognostic prediction.
The relations between the level of Mu-opioid receptor (μORs) and postoperative analgesic use in patients undergoing septoplasty: A prospective randomized controlled trial.
Authors: GENCER MUZAFFER , Göçmen Ayşe Yeşim 
Published: March 2020

Abstract
Background In this study, the μ-Opioid receptor activity was examined before the septoplasty. We assessed preoperative μ-Opioid receptor activity association with postoperative pain level and second analgesic requirement in patients undergoing septoplasty. Methods In our prospective study, 120 adult patients underwent septoplasty from June 2015 to January 2019 were randomly divided into preop 2 groups. The first group (n=60) was patients given tramadol (1-2 mgr/kg) for postop analgesia, and the second group (control group) (n=60) was initially performed only fentanyl (1 µg/ kg-i.v.) in the induction. The μ-Opioid receptors activities were investigated in preoperative blood samples and compared post-op pain level and requiring time for second analgesic agent. The visual analogue score(VAS) was used to evaluate the postoperative pain degree (0 no pain,10 worst pain). Patients' post op VAS scores were evaluated upon arrival to recovery room, and at 1st, 3rd, 7th, 10th, and 24 th hours in postoperative period. Results Demographic data and perioperative variables was similar in both study group (p<0.05). There was no significant difference between the receptor levels in both two groups and the mean receptor level was 200.94 pg/ml. While the highest receptor level was 489.92 pg/ ml, the lowest receptor level was 94.56 pg/ml. In patients who used tradomal, as the levels of μ-Opioid receptors increased, VAS scores of patients and second analgesic use decreased in post-operative period. Vas scores were lower in the tramadol group compared to the control group. Patients in Tramadol group needed second pain killer later than patients in the Control group. Conclusions In this study, we revealed that postoperative pain level and second analgesic requirement decreased as μ-Opioid receptor levels increased in tramadol group.
Is Fear of Movement (FoM) in sports related activities a latent trait?: the item response model applied to the Photographic Series of Sports Activities for Anterior Cruciate Ligament Rupture (PHOSA-ACLR).
Authors: Lankveld Wim van , Pat-El Ron J. , Melick Nicky van , Cingel Robert van , Staal J. Bart 
Published: March 2020

Abstract
Background. Fear of Movement is an important factor predicting return to sports in Anterior Cruciate Ligament Reconstruction (ACLR). Fear for specific ACLR related movements can be addressed measuring the Photographic Sports Activities for ACLR (PHOSA-ACLR). This study was conducted to determine whether Fear of Movement assessed using the PHOSA-ACLR is a latent trait, and to analyse differences in PHOSA-ACLR in athletes with or without an ACL rupture. Methods. Data from three groups of participants were included in this study: 1) ACLR patients participated in a previous study measuring psychological dimensions after ACLR (n = 58); 2) first year physical therapy students at the HAN University of Applied Sciences (n = 169), and 3) junior football players that were enrolled in a training programme of the Professional Soccer club Vitesse (Arnhem) (n = 30). All participants completed the PHOSA-ACLR (scale score 0-10), and questions related to demographics. ACLR patients completed additional questionnaires to assess functioning and Fear of Harm. PHOSA-ACLR items were analysed with Item Response Theory using the Graded Response Model (GRM). Differences between three groups of participants were analysed using Univariate Analysis of Variance. Results. The two-parameter GRM with both location and discrimination (-2LL = -5485.05) showed a significantly better fit than the one-parameter model (-2LL = -5515.36), Common.EditSubmissionSteps.Transform.EquationText (1) = 30.31, p = 0.00). The items of the PHOSA-ACLR thus constitute a latent trait. The three groups of participants differed in reported fear of movement assessed with the PHOSA-ACLR after controlling for covariates gender and age. Physical therapy students reported higher levels of fear of movement compared to either ACLR patients and healthy soccer players (respectively 4.9 (95%CI 4.6-5.3); 3.6 (95%CI 2.5-3.7); 2.6 (95%CI 1.6-3.6)). Conclusion. PHOSA-ACLR items constitute a latent trait of fear of movement for ACLR specific movements. Contrary to expectations, PHOSA-ACLR is higher in first year physiotherapy students compared to patients rehabilitating from ACLR, and healthy junior soccer players. The PHOSA-ACLR might be useful for clinicians to identify high levels of fear of movement related to ACLR, and provide a tool to help the patient overcome fear of movement using exposure techniques.
Radiographic severity index in COVID-19 pneumonia: relationship to age and sex in 783 Italian patients
Authors: Borghesi Andrea , Zigliani Angelo , Masciullo Roberto , Golemi Salvatore , Maculotti Patrizia , Farina Davide , Maroldi Roberto 
Published: March 2020

Abstract
Purpose: To improve the risk stratification of patients infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), an experimental chest x-ray (CXR) scoring system for quantifying lung abnormalities was introduced in our Diagnostic Imaging Department. The purpose of this study was to retrospectively evaluate correlations between the CXR score and the age or sex of Italian patients infected with SARS-CoV-2. Material and Methods: Between March 4, 2020 and March 18, 2020, all CXR reports containing the new scoring system were retrieved. Only hospitalized patients with SARS-CoV-2 infection were enrolled. For each patient, age, sex, and the CXR report containing the highest score were considered for the analysis. Patients were also divided into seven groups according to age. Non-parametric statistical tests were used to examine the relationship between the severity of lung disease and the age or sex.Results: 783 Italian patients (532 males and 251 females) with SARS-CoV-2 infection were enrolled. The CXR score was significantly higher in males than in females only in groups aged 50 to 79 years. A significant correlation was observed between the CXR score and age in both males and females. Males aged 50 years or older and females aged 80 years or older showed a significantly increased risk of developing severe coronavirus disease 2019 (COVID-19).Conclusions: Males aged 50 years or older and females aged 80 years or older showed the highest risk of developing severe lung disease. Our results may help to identify the highest-risk patients and those who require specific treatment strategies.
Cytoreductive surgery (CRS) with hyperthermic intraoperative peritoneal chemotherapy (HIPEC) versus standard of care (SoC) in people with peritoneal metastases from colorectal, ovarian or gastric origin: protocol for a systematic review and individual participant data (IPD) meta-analyses of effectiveness and cost-effectiveness
Authors: Gurusamy Kurinchi Selvan , Vale Claire , Pizzo Elena , Bhanot R , Davidson Brian , Mould Tim , Mughal Muntzer , Saunders Mark , Aziz Omer , O’Dwyer Sarah 
Published: March 2020

Abstract
Background There is uncertainty about whether cytoreductive surgery (CRS) + hyperthermic intraoperative peritoneal chemotherapy (HIPEC) improves survival and/or quality of life compared to standard of care (SoC) in people with peritoneal metastases who can withstand major surgery. Primary objectives To compare the relative benefits and harms of CRS+HIPEC versus SoC in people with peritoneal metastases from colorectal, ovarian, or gastric cancers eligible to undergo CRS+HIPEC by a systematic review and individual participant data (IPD) meta-analysis. Secondary objectives To compare the cost-effectiveness of CRS+HIPEC versus SoC from a National Health Service (NHS) and personal social services (PSS) perspective using a model-based cost-utility analysis. Methods We will perform a systematic review of literature by updating the searches from MEDLINE, EMBASE, Cochrane library, Science Citation Index as well as trial registers. Two members of our team will independently screen the search results and identify randomised controlled trials (RCTs) comparing CRS+HIPEC versus SoC for inclusion based on full texts for articles shortlisted during screening. We will assess the risk of bias in the trials and obtain data related to baseline prognostic characteristics, details of how CRS+HIPEC and SoC were performed, how long people were followed-up, and outcome data related to overall survival, disease progression, health-related quality of life, treatment related complications, and resource utilisation data by contacting the study authors and obtaining data at the individual participant level. Using individual participant data (IPD), we will perform a two-step IPD, i.e. calculate the adjusted effect estimate from each included study and then perform a random-effects model meta-analysis. We will perform various subgroup analyses and metaregression to investigate potential sources of heterogeneity and identify whether a subset of participants benefits from CRS+HIPEC and perform sensitivity analyses to test the robustness of results to assumptions. We will also perform a model-based cost-utility analysis to assess whether CRS+HIPEC is cost-effective in the NHS setting.
Intercept of minute ventilation vs. carbon dioxide output relationship in chronic obstructive pulmonary disease: utility as an index of ventilatory inefficiency
Authors: Wang Haoyan , Lin Fang , Nie Shan , Zhao Ranran , Cao Min , Yuan Wei , Li Yunxiao , Tan Chunting , Xu Bo 
Published: March 2020

Abstract
Background: Ventilatory inefficiency is known to be a contributor to exercise intolerance in chronic obstructive pulmonary disease (COPD). The intercept of the minute ventilation (V̇E) vs. carbon dioxide output (V̇CO2) plot is a key ventilator inefficiency parameter. However, its relationships with lung hyperinflation (LH) and airflow limitation are not known. This study aimed to evaluate the correlations between the V̇E/V̇CO2 intercept and LH in COPD to determine its utility as an index of functional impairment.Methods: We conducted a retrospective analysis of data from 53 COPD patients and 14 healthy controls performed incremental cardiopulmonary exercise tests and resting pulmonary function. Ventilatory inefficiency was represented by parameters reflecting the V̇E/V̇CO2 nadir and slope (linear region), and intercept of the V̇E/V̇CO2 plot. Their correlations with measures of LH and airflow limitation were evaluated.Results: Compared to the control, the slope (30.58±3.62) and intercept (4.85±1.11) higher in COPDstages1-2, leading to a higher nadir (31.47±4.47) (p<0.05). Despite an even higher intercept in COPDstages3-4 (7.16±1.41), the slope diminished with disease progression (from 30.58±3.62 in COPDstages1-2 to 28.36±4.58 in COPDstages3-4). Compared to the V̇E/V̇CO2 nadir and V̇E/V̇CO2 slope, the intercept was better correlated with peak V̇E/maximal voluntary ventilation (MVV) (r=0.489, p<0.001) and peak V̇O2/watt (r=0.354, p=0.003). The intercept was also significantly correlated with RV/TLC (r=0.588, p<0.001), IC/TLC (r=-0.574, p<0.001), peak VT/TLC (r=-0.585, p<0.001); and airflow limitation forced expiratory volume in 1s (FEV1) % predicted (r=-0.606, p<0.001) and FEV1/forced vital capacity (FVC) (r=-0.629, p<0.001).Conclusion: V̇E/V̇CO2intercept was consistently better correlated with worsening static and dynamic lung hyperinflation and airflow limitation in COPD. V̇E/V̇CO2 intercept emerged as a useful index of ventilatory inefficiency across the severity spectrum of COPD patients.
Macrolide and fluoroquinolone associated mutations in Mycoplasma genitalium in a retrospective study of male and female patients seeking care at an STI Clinic in Guangzhou, China, 2016-2018.
Authors: Ke Wujian , Li Dongling , Tso Lai Sze , Wei Ran , Lan Yinyuan , Chen Zhengyu , Zhang Xiaohui , Wang Liuyuan , Liang Chunmei , Liao Yuying , Chen Huiru , Liu Yahui , Zheng Heping , Yang Ligang 
Published: March 2020

Abstract
BackgroundAntimicrobial resistance in M. genitalium is a growing clinical problem. We investigated the presence of mutations for macrolide and fluoroquinolone, two commonly used medical regiments for treatments in China. Our aim is to analyze the prevalence and diversity of mutations among M. genitalium-positive clinical specimens in Guangzhou, Guangdong, south China.MethodsA total of 154 stored M. genitalium positive specimens from men and women attending an STI clinic were tested for macrolide and fluoroquinolone mutations. M. genitalium was detected via TaqMan MGB real-time PCR with a sensitivity of five genome equivalents (geq)/reaction. Mutations associated with macrolide resistance were detected using primers targeting region V of the 23S rRNA gene. Fluoroquinolone resistant mutations were screened via primers targeting topoisomerase IV (parC) and DNA gyrase (gyrA).Results98.7% (152/154), 95.5% (147/154) and 90.3% (139/154) of M. genitalium positive samples produced sufficient amplicon for detecting resistance mutations in 23S rRNA, gyrA and parC genes, respectively. 66.4% (101/152), 0.7% (1/147) and 77.7% (108/139) samples manifested mutations in 23S rRNA, gyrA and parC genes, respectively. A2072G (59/101, 58.4%) and S83I (79/108, 73.1%) were highly predominating in 23S rRNA and parC genes, respectively. Two sample had amino acid alteration in gyrA (M95I and A96T, respectively). Two sample had two amino acid alterations in parC (S83I + D87Y). 48.6% (67/138) samples harbored both macrolide and fluoroquinolone resistance-associated mutations. The most common combination of mutations was A2072G (23S rRNA) and S83I (parC) (40/67, 59.7%). One sample had three amino acid changes in 23S rRNA, gyrA and parC genes (A2072G + A96T + S83I).ConclusionsThe high antimicrobial resistance rate of M. genitalium shows a worrisome trend in Guangzhou and suggests antimicrobial resistance testing and the development of new antibiotic regimens are crucial.