bluuebunny/crossref_metadata_2025_split huggingface dataset:
Epidemiological study on calf diarrhea and coccidiosis in dairy farms in Bahir Dar, North West Ethiopia
Authors: Tamrat Habtamu , Mekonnen Negesse , Ferede Yeshwas , Cassini Rudi , Belayneh Negus 
Published: July 2020

Abstract
Background
A longitudinal prospective study was conducted from October 2017 to April 2018 on calf diarrhea and coccidiosis in dairy farms in Bahir Dar, North West Ethiopia with the objectives of determining the incidence of calf diarrhea and calf coccidiosis from diarrheic calves, assessing the major risk factors associated with calf diarrhea and coccidiosis and identifying the existing Eimeria species. A total of 237 calves, 86 calves from 52 smallholder dairy farms and 151 calves from 8 large dairy farms, were used for this study. Fresh fecal samples were collected from 86 diarrheic calves for identification of Eimeria species.

Results
Overall incidences of calf diarrhea and coccidiosis found in this study were 33.5 and 20.1%, respectively. In total, 19 potential risk factors were investigated for their association with calf diarrhea and coccidiosis from diarrheic calves using Cox regression. Age of calf (HR = 2.057, P = .002), body condition (HR = 1.802, P < .001), house condition (HR = 2.072, P = .004) and age at first colostrum feeding time (HR = 2.107, P = .002) were found significantly (P < 0.05) associated with the risk of diarrhea by multivariate Cox regression. Among the risk factors tested, age (HR = 13.36, P < .001) and sex of calves (HR = 3.500, P = .020) were found significantly (P < 0.05) associated with coccidiosis by multivariate Cox regression. A total of nine Eimeria species were identified. E. bovis (28.6%), E. zuernii (19.0%) and E. auburnensis (14.3%) were the most common Eimeria species encountered.

Conclusion
The incidence of calf diarrhea and coccidiosis was high in the dairy herds in North West Ethiopia. Therefore, sound dairy calf management practices are needed to mitigate risk factors for calf diarrhea and coccidiosis with a view to reducing the incidence of calf diarrhea and coccidiosis in Ethiopian dairy farms.
Intelligent cloud workflow management and scheduling method for big data applications
Authors: Hu Yannian , Wang Hui , Ma Wenge 
Published: July 2020

AbstractWith the application and comprehensive development of big data technology, the need for effective research on cloud workflow management and scheduling is becoming increasingly urgent. However, there are currently suitable methods for effective analysis. To determine how to effectively manage and schedule smart cloud workflows, this article studies big data from various aspects and draws the following conclusions: Compared with the original JStorm system, the response time is shortened by a maximum of 58.26% and an average of 23.18%, CPU resource utilization is increased by a maximum of 17.96% and an average of 11.39%, and memory utilization increased by a maximum of 88.7% and an average of 71.16%. In terms of optimizing the dynamic combination of web services, the overall performance of both the MOACO and CCA algorithms is better than that of the GA algorithm, and the average performance of the MOACO algorithm is better than that of the CCA algorithm. This paper also proposes a cloud workflow scheduling strategy based on an intelligent algorithm and realizes the two-tier scheduling of cloud workflow tasks by adjusting the combination strategy for cloud service resources. We have studied three representative intelligent algorithms (ACO, PSO and GA) and improved them for scheduling optimization. It can be clearly seen that in the same scenario, the optimal values of the different algorithms vary greatly for different test cases. However, the optimal solution curve is substantially consistent with the trend of the mean curve.
Characterization of a novel endolysin from bacteriophage infecting Vibrio parahaemolyticus, vB_VpaP_KF2
Authors: Lim Jeong-A , Lee Nari , Chun Hyang-Sook , Chang Hyun-Joo 
Published: July 2020

AbstractThe antimicrobial resistance of food-borne pathogenic bacteria, including Vibrio parahaemolyticus, has been reported globally, warranting the need to identify promising alternative antibiotics such as endolysins that originate from bacteriophages. In our previous study, we characterized a bacteriophage infecting V. parahaemolyticus, vB_VpaP_KF2, at the molecular level. In this study, an open reading frame encoding putative endolysin was cloned from the complete genome data and expressed in the Escherichia coli expression system. The recombinant endolysin, vB_VpaP_KF2_Lys, exhibited a novel lytic property against Gram-negative bacteria regardless of pretreatment with an outer-membrane permeabilizer. It was also stable over a wide range of temperatures, pH, and NaCl concentrations, and its hydrolytic spectrum was broader than that of the parent bacteriophage. From the results, vB_VpaP_KF2_Lys could be used as a biocontrol agent against food-borne pathogens in the field of food safety.
Diet induced the change of mtDNA copy number and metabolism in Angus cattle
Authors: Bai Ying , Carrillo José A. , Li Yaokun , He Yanghua , Song Jiuzhou 
Published: July 2020

Abstract
Background
Grass-fed and grain-fed Angus cattle differ in the diet regimes. However, the intricate mechanisms of different beef quality and other phenotypes induced by diet differences are still unclear. Diet affects mitochondrial function and dynamic behavior in response to changes in energy demand and supply. In this study, we examined the mtDNA copy number, mitochondria-related genes expression, and metabolic biomarkers in grass-fed and grain-fed Angus cattle.

Results
We found that the grass-fed group had a higher mtDNA copy number than the grain-fed group. Among different tissues, the mtDNA copy number was the highest in the liver than muscle, rumen, and spleen. Based on the transcriptome of the four tissues, a lower expression of mtDNA-encoded genes in the grass-fed group compared to the grain-fed group was discovered. For the mitochondria-related nuclear genes, however, most of them were significantly down-regulated in the muscle of the grass-fed group and up-regulated in the other three tissues. In which, COX6A2, POLG2, PPIF, DCN, and NDUFA12, involving in ATP synthesis, mitochondrial replication, transcription, and maintenance, might contribute to the alterations of mtDNA copy number and gene expression. Meanwhile, 40 and 23 metabolic biomarkers were identified in the blood and muscle of the grain-fed group compared to a grass-fed group, respectively. Integrated analysis of the altered metabolites and gene expression revealed the high expression level of MDH1 in the grain-fed group might contribute to the mitochondrial NADH oxidation and spermidine metabolism for adapting the deletion mtDNA copy number.

Conclusions
Overall, the study may provide further deep insight into the adaptive and regulatory modulations of the mitochondrial function in response to different feeding systems in Angus cattle.
Gravity stress tibiotalar laxity evaluation with a biomedical gyroscopes device – cadaver study with progressive sectioning of lateral ankle ligaments
Authors: Guerra-Pinto F. , Cunha J. , Sousa L. , Gomes T. M. , Andrade R. , Oliva X. M. , Consciência J. G. , Fernandes P. R. 
Published: July 2020

Abstract
Purpose
Despite the evidence on the role of gravity stress test to access the instability of other ankle injuries, there is limited literature regarding gravity stress on the lateral ankle ligament’s insufficiency. The objective of our study was to objectively measure the tibiotalar angular movement under gravity stress after progressive sectioning of the lateral ankle ligaments.

Methods
We performed sequential sectioning of the anterior talofibular (ATFL), calcaneofibular (CFL), and posterior talofibular ligaments (PTFL) in twelve ankle specimens. Under gravity stress, we measured the angular movement of the talus in relation to the tibia. The measuring device is based on a three-axis gyroscope and accelerometer.

Results
Comparing to the intact condition, the plantar flexion increased on average 1.78° (95% confidence interval [CI] 1.15;2.42), 5.13° (95%CI 3.10;7.16) and 8.63° (95%CI 6.05;11.22), the rotation increased by 1.00° (95 CI -0.51;2.51), 3.68° (95%CI 1.97;5.40) and 15.62° (95%CI 10.09;21.14), and the varus increased 2.89° (95% CI 1.39, 4.39), 8.12° (95% CI 5.16, 11.07) and 11.68° (95% CI 7.91, 15.46), after sectioning the ATFL, CFL, and PTFL, respectively. The overall changes were statistically significant.

Conclusions
There was a significant tibiotalar laxity after sectioning of lateral ankle ligaments when the foot position is influenced only by gravity. The tibiotalar angular displacement was significant when the CFL and PTFL were cut which suggests that the gravity test could be used to assess combined lateral ankle ligament injury. This evidence might be a step forward in the development of lateral ankle ligaments gravity stress tests.

Level of evidence
5 (cadaver study)
Internet-based cognitive behavior therapy for problem gambling in routine care: protocol for a non-randomized pilot and feasibility trial
Authors: Molander Olof , Lindner Philip , Ramnerö Jonas , Bjureberg Johan , Carlbring Per , Berman Anne H. 
Published: July 2020

Abstract
Background
Problem gambling and gambling disorder are major public health concerns worldwide, and awareness of associated negative consequences is rising. In parallel, treatment demand has increased, and Internet interventions offer a promising alternative for providing evidence-based treatment at scale to a low cost.

Method
We developed a novel Internet-delivered cognitive behavioral treatment for gambling, based on qualitative interviews with treatment-seeking gamblers, behavioral research on gambling behavior, and the pathway model for problem gambling. This research protocol describes a non-randomized pilot and feasibility trial conducted in routine addiction care with adult treatment-seeking patients (max N = 25) with problem gambling. The primary aim is to ensure acceptability and safety, measured by satisfaction, credibility, working alliance, and possible negative effects. Secondary aims are feasibility of study procedures in terms of recruitment and measurement procedures as well as potential effectiveness measured weekly by gambling symptoms as primary outcome and gambling behavior, quality of life, symptoms of depression and anxiety, alcohol, and drug use as secondary outcomes. Potential mediators measured weekly are loss of control, verbal rules, and well-being.

Discussion
This study is innovative in several respects, regarding both treatment development and implementation. The results of the study will guide a future randomized controlled trial, as well as the development of the intervention and intervention implementation within ordinary addiction care.

Trial registration
Clinical trials.gov, NCT ID: NCT03946098. Registered 10 May 2019
Freedom to think: the need for thorough assessment and treatment of gender dysphoric children
Authors: Evans Marcus 
Published: July 2020

SummaryReferrals (particularly natal female) to gender identity clinics have increased significantly in recent years. Understanding the reasons for this increase, and how to respond, is hampered by a politically charged debate regarding gender identity. This article starts with a discussion of the so-called ‘affirmative approach’ to gender dysphoria and considers the implications of the Memorandum of Understanding on conversion therapy. I then say something about the relationship between gender dysphoria and the developmental problems that are characteristic of adolescence. Finally, I outline what changes to the current approach are needed to do our best to ensure these patients receive the appropriate treatment.
Sex, gender and gender identity: a re-evaluation of the evidence
Authors: Griffin Lucy , Clyde Katie , Byng Richard , Bewley Susan 
Published: July 2020

SummaryIn the past decade there has been a rapid increase in gender diversity, particularly in children and young people, with referrals to specialist gender clinics rising. In this article, the evolving terminology around transgender health is considered and the role of psychiatry is explored now that this condition is no longer classified as a mental illness. The concept of conversion therapy with reference to alternative gender identities is examined critically and with reference to psychiatry's historical relationship with conversion therapy for homosexuality. The authors consider the uncertainties that clinicians face when dealing with something that is no longer a disorder nor a mental condition and yet for which medical interventions are frequently sought and in which mental health comorbidities are common.
Prenatal EDCs Impair Mate and Odor Preference and Activation of the VMN in Male and Female Rats
Authors: Hernandez Scudder Morgan E , Weinberg Amy , Thompson Lindsay , Crews David , Gore Andrea C 
Published: July 2020

AbstractEnvironmental endocrine-disrupting chemicals (EDCs) disrupt hormone-dependent biological processes. We examined how prenatal exposure to EDCs act in a sex-specific manner to disrupt social and olfactory behaviors in adulthood and underlying neurobiological mechanisms. Pregnant rat dams were injected daily from embryonic day 8 to 18 with 1 mg/kg Aroclor 1221 (A1221), 1 mg/kg vinclozolin, or the vehicle (6% DMSO in sesame oil). A1221 is a mixture of polychlorinated biphenyls (weakly estrogenic) while vinclozolin is a fungicide (anti-androgenic). Adult male offspring exposed to A1221 or vinclozolin, and females exposed to A1221, had impaired mate preference behavior when given a choice between 2 opposite-sex rats that differed by hormone status. A similar pattern of impairment was observed in an odor preference test for urine-soaked filter paper from the same rat groups. A habituation/dishabituation test revealed that all rats had normal odor discrimination ability. Because of the importance of the ventrolateral portion of the ventromedial nucleus (VMNvl) in mate choice, expression of the immediate early gene product Fos was measured, along with its co-expression in estrogen receptor alpha (ERα) cells. A1221 females with impaired mate and odor preference behavior also had increased neuronal activation in the VMNvl, although not specific to ERα-expressing neurons. Interestingly, males exposed to EDCs had normal Fos expression in this region, suggesting that other neurons and/or brain regions mediate these effects. The high conservation of hormonal, olfactory, and behavioral traits necessary for reproductive success means that EDC contamination and its ability to alter these traits has widespread effects on wildlife and humans.
Influence of Cinnamon on Glycemic Control in Individuals With Prediabetes: A Randomized Controlled Trial
Authors: Romeo Giulio R , Lee Junhee , Mulla Christopher M , Noh Youngmin , Holden Casey , Lee Byung-Cheol 
Published: July 2020

Abstract

Context
The identification of adjunct safe, durable, and cost-effective approaches to reduce the progression from prediabetes to type 2 diabetes (T2D) is a clinically relevant, unmet goal. It is unknown whether cinnamon’s glucose-lowering properties can be leveraged in individuals with prediabetes.

Objective
The objective of this work is to investigate the effects of cinnamon on measures of glucose homeostasis in prediabetes.

Design, Setting, Participants, and Intervention
This double-blind, placebo-controlled, clinical trial randomly assigned adult individuals meeting any criteria for prediabetes to receive cinnamon 500 mg or placebo thrice daily (n = 27/group). Participants were enrolled and followed at 2 academic centers for 12 weeks.

Main Outcome Measures
Primary outcome was the between-group difference in fasting plasma glucose (FPG) at 12 weeks from baseline. Secondary end points included the change in 2-hour PG of the oral glucose tolerance test (OGTT), and the change in the PG area under the curve (AUC) derived from the OGTT.

Results
From a similar baseline, FPG rose after 12 weeks with placebo but remained stable with cinnamon, leading to a mean between-group difference of 5 mg/dL (P &lt; .05). When compared to the respective baseline, cinnamon, but not placebo, resulted in a significant decrease of the AUC PG (P &lt; .001) and of the 2-hour PG of the OGTT (P &lt; .05). There were no serious adverse events in either study group.

Conclusions
In individuals with prediabetes, 12 weeks of cinnamon supplementation improved FPG and glucose tolerance, with a favorable safety profile. Longer and larger studies should address cinnamon’s effects on the rate of progression from prediabetes to T2D.
The structure and symmetry of the radial spoke protein complex in Chlamydomonas flagella
Authors: Poghosyan Emiliya , Iacovache Ioan , Faltova Lenka , Leitner Alexander , Yang Pinfen , Diener Dennis R. , Aebersold Ruedi , Zuber Benoit , Ishikawa Takashi 
Published: July 2020

ABSTRACT
The radial spoke is a key element in a transducer apparatus controlling the motility of eukaryotic cilia. The transduction biomechanics is a long-standing question in cilia biology. The radial spoke has three regions – a spoke head, a bifurcated neck and a stalk. Although the neck and the stalk are asymmetric, twofold symmetry of the head has remained controversial. In this work we used single particle cryo-electron microscopy (cryo-EM) analysis to generate a 3D structure of the whole radial spoke at unprecedented resolution. We show the head region at 15 Å (1.5 nm) resolution and confirm twofold symmetry. Using distance constraints generated by cross-linking mass spectrometry, we locate two components, RSP2 and RSP4, at the head and neck regions. Our biophysical analysis of isolated RSP4, RSP9, and RSP10 affirmed their oligomeric state. Our results enable us to redefine the boundaries of the regions and propose a model of organization of the radial spoke component proteins.
The ubiquitin-like modifier FAT10 – much more than a proteasome-targeting signal
Authors: Aichem Annette , Groettrup Marcus 
Published: July 2020

ABSTRACT
Human leukocyte antigen (HLA)-F adjacent transcript 10 (FAT10) also called ubiquitin D (UBD) is a member of the ubiquitin-like modifier (ULM) family. The FAT10 gene is localized in the MHC class I locus and FAT10 protein expression is mainly restricted to cells and organs of the immune system. In all other cell types and tissues, FAT10 expression is highly inducible by the pro-inflammatory cytokines interferon (IFN)-γ and tumor necrosis factor (TNF). Besides ubiquitin, FAT10 is the only ULM which directly targets its substrates for degradation by the 26S proteasome. This poses the question as to why two ULMs sharing the proteasome-targeting function have evolved and how they differ from each other. This Review summarizes the current knowledge of the special structure of FAT10 and highlights its differences from ubiquitin. We discuss how these differences might result in differential outcomes concerning proteasomal degradation mechanisms and non-covalent target interactions. Moreover, recent insights about the structural and functional impact of FAT10 interacting with specific non-covalent interaction partners are reviewed.
Prognostic Value of Leg Muscle Strength in Acute Heart Failure Syndrome
Authors: YOUN JONG-CHAN , CHOI SUK-WON , LEE HYE SUN , HAN SEONGWOO , SHIN EUI-CHEOL , BAEK SANG HONG , KANG SEOK-MIn 
Published: July 2020

ABSTRACT

Introduction
Leg muscle strength (LMS) may be useful as a frailty index in patients with heart failure. However, LMS, until recently, has been indirectly estimated, and its prognostic value in acute heart failure syndrome (AHFS) is unclear. Therefore, we evaluated the prognostic value of direct LMS assessment and its relationship with proinflammatory mediators in patients with AHFS.

Methods
We directly measured LMS at predischarge using a dynamometer in 110 prospectively and consecutively enrolled patients with AHFS (75 male; 60 ± 14 yr; mean ejection fraction, 29.9% ± 14.6%). The primary end point was cardiovascular (CV) events, defined as CV mortality, cardiac transplantation, or rehospitalization due to heart failure aggravation. Patients were divided into impaired and preserved LMS groups according to Contal and O’Quigley’s method.

Results
CV events occurred in 28 patients (25.5%) (including 5 CV deaths and 6 cardiac transplantations) during follow-up (median, 246 d; range = 11–888 d). Impaired LMS was associated with significantly higher levels of serum monokine induced by gamma interferon and poor clinical outcomes (P < 0.001). Multivariable Cox proportional hazard analysis (controlling for age, sex, body mass index, heart failure type, hemoglobin level, N-terminal pro-b-type natriuretic peptide level, and beta-blocker use) revealed LMS as an independent predictor of CV events (P = 0.017).

Conclusion
Impaired LMS, which might be used as a marker of frailty, is associated with increased levels of a proinflammatory chemokine and independently predicts clinical outcomes in patients with AHFS. The direct measurement of LMS is simple and feasible and might have important implications for the risk stratification of patients with AHFS.
HRR and V˙O2R Fractions Are Not Equivalent: Is It Time to Rethink Aerobic Exercise Prescription Methods?
Authors: FERRI MARINI CARLO , SISTI DAVIDE , LEON ARTHUR S. , SKINNER JAMES S. , SARZYNSKI MARK A. , BOUCHARD CLAUDE , ROCCHI MARCO B. L. , PICCOLI GIOVANNI , STOCCHI VILBERTO , FEDERICI ARIO , LUCERTINI FRANCESCO 
Published: July 2020

ABSTRACT

Introduction
According to current guidelines, the intensity of health-enhancing aerobic exercise should be prescribed using a percentage of heart rate reserve (%HRR), which is considered to be more closely associated (showing a 1:1 relation) with the percentage of oxygen uptake reserve (%V˙O2R) rather than with the percentage of maximal oxygen uptake (%V˙O2max) during incremental exercise. However, the associations between %HRR and %V˙O2R and between %HRR and %V˙O2max are under debate; hence, their actual relationships were investigated in this study.

Methods
Data from each stage of a maximal incremental exercise test performed by 737 healthy and physically inactive participants of the HERITAGE Family Study were screened and filtered then used to calculate the individual linear regressions between %HRR and either %V˙O2R or %V˙O2max. For each relationship, the mean slope and intercept of the individual linear regression were compared with 1 and 0 (i.e., the identity line), respectively, using one-sample t-tests. The individual root mean square errors of the actual versus the 1:1 predicted %HRR were calculated for both relationships and compared using a paired-sample t-test.

Results
The mean slopes (%HRR–%V˙O2R, 0.972 ± 0.189; %HRR–%V˙O2max, 1.096 ± 0.216) and intercepts (%HRR–%V˙O2R, 8.855 ± 16.022; %HRR–%V˙O2max, −3.616 ± 18.993) of both relationships were significantly different from 1 and 0, respectively, with high interindividual variability. The average root mean square errors were high and revealed that the %HRR–%V˙O2max relationship was more similar to the identity line (P < 0.001) than the %HRR–%V˙O2R relationship (7.78% ± 4.49% vs 9.25% ± 5.54%).

Conclusions
Because both relationships are different from the identity line and using a single equation may not be appropriate to predict exercise intensity at the individual level, a rethinking of the relationships between the intensity variables may be necessary to ensure that the most suitable health-enhancing aerobic exercise intensity is prescribed.