bluuebunny/crossref_metadata_2025_split huggingface dataset:
Autophagic signaling promotes systems-wide remodeling in skeletal muscle upon oncometabolic stress
Authors: Karlstaedt Anja , Vitrac Heidi , Salazar Rebecca L. , Gould Benjamin D. , Soetkamp Daniel , Spivia Weston , Raedschelders Koen , Dinh An Q. , Guzman Anna G. , Tan Lin , Azinas Stavros , Taylor David J.R. , Schiffer Walter , McNavish Daniel , Burks Helen B. , Gottlieb Roberta A. , Lorenzi Philip L. , Hanson Blake M. , Van Eyk Jennifer E. , Taegtmeyer Heinrich 
Published: October 2020

AbstractAbout 20-30% of cancer-associated deaths are due to complications from cachexia which is characterized by skeletal muscle atrophy. Metabolic reprogramming in cancer cells causes body-wide metabolic and proteomic remodeling, which remain poorly understood. Here, we present evidence that the oncometabolite D-2-hydroxylgutarate (D2-HG) impairs NAD+redox homeostasis in skeletal myotubes, causing atrophy via deacetylation of LC3-II by the nuclear deacetylase Sirt1. Overexpression of p300 or silencing of Sirt1 abrogate its interaction with LC3, and subsequently reduced levels of LC3 lipidation. Using RNA-sequencing and mass spectrometry-based metabolomics and proteomics, we demonstrate that prolonged treatment with the oncometabolite D2-HG in mice promotes cachexiain vivoand increases the abundance of proteins and metabolites, which are involved in energy substrate metabolism, chromatin acetylation and autophagy regulation. We further show that D2-HG promotes a sex-dependent adaptation in skeletal muscle using network modeling and machine learning algorithms. Our multi-omics approach exposes new metabolic vulnerabilities in response to D2-HG in skeletal muscle and provides a conceptual framework for identifying therapeutic targets in cachexia.
Early Prediction of COVID-19 Severity Using Extracellular Vesicles and Extracellular RNAs
Authors: Fujita Yu , Hoshina Tokio , Matsuzaki Juntaro , Kadota Tsukasa , Fujimoto Shota , Kawamoto Hironori , Watanabe Naoaki , Sawaki Kenji , Sakamoto Yohei , Miyajima Makiko , Lee Kwangyole , Nakaharai Kazuhiko , Horino Tetsuya , Nakagawa Ryo , Araya Jun , Miyato Mitsuru , Yoshida Masaki , Kuwano Kazuyoshi , Ochiya Takahiro 
Published: October 2020

AbstractThe clinical manifestations of COVID-19 vary broadly, ranging from asymptomatic infection to acute respiratory failure and death. But the predictive biomarkers for characterizing the variability are still lacking. Since emerging evidence indicates that extracellular vesicles (EVs) and extracellular RNAs (exRNAs) are functionally involved in a number of pathological processes, we hypothesize that these extracellular components may be key determinants and/or predictors of COVID-19 severity. To test our hypothesis, we collected serum samples from 31 patients with mild COVID-19 symptoms at the time of their admission. After standard therapy without corticosteroids, 9 of the 31 patients developed severe COVID-19 symptoms. We analyzed EV protein and exRNA profiles to look for correlations between these profiles and COVID-19 severity. Strikingly, we identified three distinct groups of markers (antiviral response-related EV proteins, coagulation-related markers, and liver damage-related exRNAs) with the potential to serve as early predictive biomarkers for COVID-19 severity. Among these markers, EV COPB2 has the best predictive value for severe deterioration of COVID-19 patients in this cohort. This type of information concerning functional extracellular component profiles could have great value for patient stratification and for making early clinical decisions about strategies for COVID-19 therapy.
Genotype identification and diversity analysis in Kiwifruit (Actinidiaspp.) using RAPD markers
Authors: Kamboj Aarti , Kharb Pushpa , Jhilta Agrim , Singh Rakshita 
Published: October 2020

AbstractKiwifruit (Actinidia spp.) is a significant plantation crop belonging to family Actinidiaceae, having deciduous, dioecious, and scrambling vines with chromosome number 2n=58. Dioecy in kiwifruit forms the basis for several breeding programs. The present study was carried out for diversity analysis in kiwifruit genotypes using RAPD markers. 7 kiwifruit genotypes (2 males viz. Allision & Tomuri and 5 females viz. Hayward, Bruno, Allision, Monty & Abbott) were analysed for molecular polymorphism using 94 RAPD primers, out of which 23 primers amplified the genomic DNA in all the genotypes. RAPD data was analysed using NTSYS-pc software and dendrogram construction was done using UPGMA method. Two separate clusters of male and female genotypes were formed. Similarity matrix indices showed maximum similarity between Tomuri (M) and Allision (M) with a similarity coefficient of 0.719 while Abbott (F) and Allision (M) were found to have least similarity having a similarity coefficient of 0.521. Four RAPD primers amplified unique amplicons in Monty, Hayward, Bruno, Allision (M) and Abbott and two primers amplified unique amplicons in Allision (M) & Tomuri (M) along with the male and female plants of Allision genotype respectively. Therefore, these primers can help in distinguishing the genotypes of kiwifruit and can also be validated as putative markers for the sex identification in kiwifruit.
Pancreatic β-cell specific loss ofE2f1impairs insulin secretion and β-cell identity through the epigenetic repression of non β-cell programs
Authors: Oger Frédérik , Bourouh Cyril , Gromada Xavier , Moreno Maeva , Carney Charlène , Courty Emilie , Rabhi Nabil , Durand Emmanuelle , Amanzougarene Souhila , Berberian Lionel , Derhourhi Mehdi , Rolland Laure , Hannou Sarah Anissa , Denechaud Pierre-Damien , Benfodda Zohra , Meffre Patrick , Fajas Lluis , Kerr-Conte Julie , Pattou François , Froguel Philippe , Bonnefond Amélie , Annicotte Jean-Sébastien 
Published: October 2020

AbstractThe loss of pancreatic β-cell identity emerges as an important feature of type 2 diabetes development, but the molecular mechanisms are still elusive. Here, we explore the cell-autonomous role of the cell cycle regulator and transcription factor E2F1 in the maintenance of β-cell identity and insulin secretion. We show that the β-cell-specific loss ofE2f1function in mice triggers glucose intolerance associated with defective insulin secretion, an altered α-to-β-cell ratio, a downregulation of many β-cell genes and a concomitant increase of non-β-cell markers. Mechanistically, the epigenomic profiling of non-beta cell upregulated gene promoters identified an enrichment of bivalent H3K4me3/H3K27me3 or H3K27me3 marks. Conversely, downregulated genes were enriched in active chromatin H3K4me3 and H3K27ac histone marks. We find that histone deacetylase inhibitors modulate E2F1 transcriptional and epigenomic signatures associated with these β-cell dysfunctions. Finally, the pharmacological inhibition of E2F transcriptional activity in human islets also impairs insulin secretion and the expression of β-cell identity genes. Our data suggest that E2F1 is critical for maintaining β-cell identity through a sustained repression of non β-cell transcriptional programs.
Repurposed antiviral drugs for COVID-19 –interim WHO SOLIDARITY trial results
Authors:   WHO Solidarity trial consortium, Pan Hongchao , Peto Richard , Karim Quarraisha Abdool , Alejandria Marissa , Henao-Restrepo Ana Maria , García César Hernández , Kieny Marie-Paule , Malekzadeh Reza , Murthy Srinivas , Preziosi Marie-Pierre , Reddy Srinath , Periago Mirta Roses , Sathiyamoorthy Vasee , Røttingen John-Arne , Swaminathan Soumya ,   as the members of the Writing Committee, assume responsibility for the content and integrity of this article
Published: October 2020

ABSTRACTBACKGROUNDWHO expert groups recommended mortality trials in hospitalized COVID-19 of four re-purposed antiviral drugs.METHODSStudy drugs were Remdesivir, Hydroxychloroquine, Lopinavir (fixed-dose combination with Ritonavir) and Interferon-β1a (mainly subcutaneous; initially with Lopinavir, later not). COVID-19 inpatients were randomized equally between whichever study drugs were locally available and open control (up to 5 options: 4 active and local standard-of-care). The intent-to-treat primary analyses are of in-hospital mortality in the 4 pairwise comparisons of each study drug vs its controls (concurrently allocated the same management without that drug, despite availability). Kaplan-Meier 28-day risks are unstratified; log-rank death rate ratios (RRs) are stratified for age and ventilation at entry.RESULTSIn 405 hospitals in 30 countries 11,266 adults were randomized, with 2750 allocated Remdesivir, 954 Hydroxychloroquine, 1411 Lopinavir, 651 Interferon plus Lopinavir, 1412 only Interferon, and 4088 no study drug. Compliance was 94-96% midway through treatment, with 2-6% crossover. 1253 deaths were reported (at median day 8, IQR 4-14). Kaplan-Meier 28-day mortality was 12% (39% if already ventilated at randomization, 10% otherwise). Death rate ratios (with 95% CIs and numbers dead/randomized, each drug vs its control) were: Remdesivir RR=0.95 (0.81-1.11, p=0.50; 301/2743 active vs 303/2708 control), Hydroxychloroquine RR=1.19 (0.89-1.59, p=0.23; 104/947 vs 84/906), Lopinavir RR=1.00 (0.79-1.25, p=0.97; 148/1399 vs 146/1372) and Interferon RR=1.16 (0.96-1.39, p=0.11; 243/2050 vs 216/2050). No study drug definitely reduced mortality (in unventilated patients or any other subgroup of entry characteristics), initiation of ventilation or hospitalisation duration.CONCLUSIONSThese Remdesivir, Hydroxychloroquine, Lopinavir and Interferon regimens appeared to have little or no effect on hospitalized COVID-19, as indicated by overall mortality, initiation of ventilation and duration of hospital stay. The mortality findings contain most of the randomized evidence on Remdesivir and Interferon, and are consistent with meta-analyses of mortality in all major trials. (Funding: WHO. Registration: ISRCTN83971151, NCT04315948)
Trans-contextualized cross-border student choice under “one country, two systems” from Hong Kong to Mainland China for higher education
Authors: Te Alice Y.C. 
Published: October 2020

PurposeThis article examines the complexity and dynamics of Hong Kong students' choice to pursue undergraduate education in Mainland China under the context of “one country, two systems.”Design/methodology/approachThis study mainly draws on primary data from in-depth interviews of 51 Hong Kong students studying in Mainland universities. Qualitative approach of inquiry is adopted to probe into the underlying reasons and processes undergone during the educational choice.FindingsAcademic factors push Hong Kong students away from local universities due to the failure of getting admissions in preferred programs or universities, and pull them toward Mainland universities due to attractions of specific universities and programs. During three phases of decision-making process, different influencing factors have conditioned three orientations: pragmatists enrolled in their aspired programs as the first choice, achievers utilized a fast-track path to enter elite universities and underachievers treated such study opportunity as a last resort for a degree.Originality/valueThis article contributes to a better understanding of the dynamics of educational choice to higher education in Hong Kong at a strategic point of time. It explains how the macro political/policy and sociocultural contexts, the institutional influences of hosting universities and sending secondary schools, perceived parental influence and students' personal characteristics have shaped students' college choice.
Spare parts inventory management: a new hybrid approach
Authors: Muniz Leandro Reis , Conceição Samuel Vieira , Rodrigues Lásara Fabrícia , de Freitas Almeida João Flávio , Affonso Tãssia Bolotari 
Published: October 2020

PurposeThe purpose of this paper is to present a new hybrid approach based on criticality analysis and optimisation to deal with spare parts inventory management in the initial provisioning phase in the mining industry. Spare parts represent a significant part of mining companies' expenditures, so it is important to develop new approaches to reduce the total inventory value of these items.Design/methodology/approachThis hybrid approach combines qualitative and quantitative methods based on VED (vital, essential and desirable) analysis, analytical hierarchical process (AHP), and e-constraint optimisation method to obtain the spare parts to be stocked. The study was applied to a large mining company. The mineral sector was chosen due to the great importance to the emerging Brazilian economy and the lack of researches in this sector. In addition, the spare parts have a relevant weight on the total inventory cost.FindingsPresent a novel approach combining multi-objective optimisation and multi-criteria evaluation approaches to tackle the inventory decision in spare parts management. This work also defines and classifies relevant criteria for spare parts management in the mineral sector validated by specialists. The proposed approach achieves an average increase of 20.2% in the criticality and 16.6% in the number of items to be stocked compared to the historical data of the surveyed company.Research limitations/implicationsThis paper applies the proposed approach to a mining company in Brazil. Future research in other companies or regions should analyse the adequacy of the criticality criteria, hierarchy and weights adopted in this paper.Practical implicationsThe proposed approach is useful for mining industries that deal with a large variety of resource constraints as it helps in formulating appropriate spare part strategies to rationalise financial resources at both tactical and strategic levels.Originality/valueThe paper presents a new hybrid method combining the AHP a multi-criteria decision making (MCDM) approach coupled with e-constraint optimisation to deal with spare parts inventory management allowing for a better spare parts inventory analysis in the initial provisioning phase and providing managers with a systematic tool to analyse the trade-off between spare parts criticality and total inventory value.
Analysis of readiness factors for Industry 4.0 implementation in SMEs using COPRAS
Authors: Sriram R.M. , Vinodh S. 
Published: October 2020

PurposeThe purpose of this paper is to analyse the readiness factors for Industry 4.0 deployment in small and medium-sized enterprises (SMEs) using complex proportionality assessment (COPRAS) methodology.Design/methodology/approachIndustry 4.0 is vital for ensuring manufacturing competitiveness of SMEs. In order to facilitate Industry 4.0 adoption in SMEs, readiness factors need to be analysed. In this viewpoint, this article presents the analysis of readiness factors using multi-criteria decision-making (MCDM) tool COPRAS.FindingsThe priority of ranking of 15 readiness factors is obtained. The prioritized readiness factors would enable the smooth adoption of Industry 4.0 in SMEs.Practical implicationsThe study has been done based on inputs from industry practitioners and hence the results have practical relevance.Originality/valueThe analysis of readiness factors for Industry 4.0 in SMEs using COPRAS is the original contribution of authors.
The mythologisation of key workers: occupational prestige gained, sustained... and lost?
Authors: De Camargo Camilla R. , Whiley Lilith A. 
Published: October 2020

PurposeKey workers are deemed “essential” for keeping the country going while the rest of us have been resigned to the safety of our homes. Throughout the COVID-19 pandemic, key workers have become the exalted symbol of the pandemic; although, during pre-pandemic many of these roles were considered “low skilled” and were (and still are) low paid.Design/methodology/approachThe analysis uses newspapers as data sources to discuss occupational prestige and situate it within previous theory.FindingsThis social commentary discusses how occupational prestige evolves and devolves during times of social change, and how elevated status, dependent on one's service to the country in the spirit of a “war-time” narrative, is just temporary and fleeting. Prestige is wrapped up in notions of class, income and education, and during the pandemic, “key workers” have become their own sub-group with an almost mythologised status and value, which the authors argue might take the focus away from genuine efforts to improve working conditions (e.g. access to PPE and pay rises etcetera).Originality/valueThe article considers the current value of key workers and how elevated levels of prestige are transitory. The enduring nature of this new status is yet to be seen. More qualitative nuanced research is required around how occupational prestige changes, evolves and devolves and more quantitative research on why and how widespread some of the critical issues might be.
Inventory management with online payment and preorder discounts
Authors: Hasan Md. Rakibul , Daryanto Yosef , Roy Tutul Chandra , Feng Yi 
Published: October 2020

PurposeThe advancement of technology opens many opportunities for retailing businesses to increase their profit through innovative strategies, such as discount offers, preorder programs and online payment services. The purpose of this study is to investigate decision-making methods for retailers who sell deteriorating products that utilize an e-commerce platform and offering preorder.Design/methodology/approachThe authors study the optimum price and replenishment cycle when multiple discounts policy is implemented for customers when they purchase during the preorder period and make the payment via an online system. The proposed economic order quantity model works for noninstantaneous deteriorating items that will maximize the total profit. Moreover, it considers the effect of selling price and advertisement on customer demand. The concavity of the profit function is proved. Then, a comparison is carried out between the traditional payment system and online payment. Finally, two numerical examples and the sensitivity analysis are performed.FindingsThe results show the benefit of the system with online payment compared to the traditional one. Further analysis shows that the total profit increases when the frequency of advertisement, interest from the banking company, location perimeter and the nondeterioration time increase.Originality/valueThe proposed model guides e-commerce retailers optimizing the price and inventory decision when they offer a discount, preorder program and online payment service. No researcher has undergone a study with this complexity.
Productivity growth in India's bakery manufacturing industry
Authors: Kumar Shiv , Abdulla  , Singh ChhatraPal 
Published: October 2020

PurposeThe main aim of this paper is to examine the total factor productivity (TFP) and its components namely, technological change, technical efficiency change, scale change and allocative change in bakery industry in India.Design/methodology/approachThe study is based on panel data on 35 Indian states for the period 2009–2010 and 2012–2013. Stochastic frontier function is employed to estimate the productivity growth.FindingsThe results show that TFP is driven by technological progress, followed by technical efficiency and scale efficiency. Allocative efficiency, however, has a negative effect on TFP.Research limitations/implicationsThe bakery industry needs to define its innovation strategies, as these strategies lead to different outcomes that can be achieved only through the management of resources dedicated to the generation and implementation of innovations.Originality/valueUsing frontier production function takes the stochastic context into account for the dynamic behaviour of TFP and its components. Most of the past studies have assessed the TFP at the aggregate level using three-digit National Industrial Classification (NIC) or four-digit NIC code. An analysis at higher levels aggregation masks the variation in TFP and its components available at the firm level. This study uses five-digit NIC data to measure the firm specific TFP of bakery industry. Further, it looks at the contribution of technical progress (TP), technical efficiency, scale efficiency and allocative efficiency.
Impact of wise leadership, workplace friendships on open innovation in family firms: a developing country perspective
Authors: Abdulmuhsin Amir A. , Tarhini Ali 
Published: October 2020

PurposeThis study draws upon the hybrid approach of the resource-based view and social capital theory, and aims to develop and empirically validate a model that examines the relationship amongst wise leadership, workplace friendships and open innovation (OI) in family firms (FFs).Design/methodology/approachAn online survey was conducted to collect data from a sample of 381 firms from a developing country. Additionally, this study used AMOS software and structural equation model to empirically test the proposed hypotheses of the theoretical model.FindingsFindings show that wise leadership has a significant, positive indirect effect on stimulating OI in FFs via its influence on building workplace friendships and overcoming knowledge–strategic and collaboration–organisational challenges.Practical implicationsTo improve OI, top management teams of family businesses should encourage wise, intelligent, well-informed and strong leaders who drive change. Moreover, they should establish small group, “smart-world” networks for specialised innovation to facilitate friendship based on trust and competence, and develop the coordinating role of family leaders in these networks.Originality/valueThis study complements and advances previous research on OI in many ways. Firstly, the current study proposes a conceptual model that demonstrates the interrelationships amongst the main variables in Iraqi FFs. Secondly, this research explores the crucial mediating role of workplace friendship, which capitalises on the principles of friendship in the context of the acquisition, accumulation and exchange of knowledge, thereby overcoming the challenges associated with innovation.
The importance of financial resources and ownership of intellectual property rights for university spin-offs: the cases of Finland and Sweden
Authors: Kulkov Ignat , Berggren Björn , Eriksson Kent , Hellström Magnus , Wikstrom Kim 
Published: October 2020

PurposeThis paper focuses on medical device university spin-offs (USOs), taking into account the peculiarities of financial and nonfinancial support and intellectual property rights (IPRs). The authors declare that these parameters play a significant role in business development at the early stages.Design/methodology/approachThis empirical data consist of individual and group interviews in Finland and Sweden, which are later inductively analyzed.FindingsThe results show that public financial support contributes to the formation and start of sales stages in small countries and local markets. However, at the validation stage, approaches for supporting entrepreneurship in the field of medical devices may differ. The ownership of IPRs assists in the development of entrepreneurship in the region due to the transfer of research results and researchers to the industry and increases the number of spin-offs and the cooperation of universities with business.Originality/valueThis contribution is in the identification of the key parameters for the formation, support and development of the USOs from the point of view of the availability of financial resources and the ownership of IPRs.