bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development and application of green and sustainable analytical methods for flavonoid extraction from Passiflora waste
Authors: da Silva Francischini Danielle , Lopes Ana Paula , Segatto Mateus Lodi , Stahl Aylon Matheus , Zuin Vânia Gomes 
Published: September 2020

AbstractBrazilian biodiversity and favourable environmental conditions open up possibilities not yet explored, showing potential to shift the country’s monochromatic economy into an emancipated, diversified and sustainable economic environment. This can be made possible through the integral use of its resources, exploring every functional fraction to create novel solutions to modern problems. Biorefineries present an interesting strategy to fully use the potential of agricultural feedstocks and together with green separation methods can contribute to the generation of sustainable processes and products. Passion Fruit (Passiflora edulis Sims f. flavicarpa Deg species) is produced on a large scale in Brazil and in other tropical countries, and its processing plants generate tons of residues that basically consist of peel, seeds and bagasse, which account for around 75% of its mass. These fractions of P. edulis can contain significant amounts of flavonoids, secondary metabolites that are the main compounds responsible for the fruit’s bioactivity (antioxidant, anti-inflammatory, pesticide and biocide, in general). Therefore, this work aims to develop, apply and compare the best conditions for the extraction of isoorientin, orientin and isovitexin from passion fruit applying solid–liquid methodologies, followed by analyte quantification using UHPLC-PDA. Homogenizer-assisted (HAE), ultrasound-assisted (UAE) and microwave-assisted (MAE) extraction techniques were used, as well as a full factorial design to reach optimal parameters concerning the extraction yield and energy and solvent efficiencies. According to the results, the procedure based on HAE presented the best conditions for the extraction of selected flavonoids (1.07, 0.90 and 0.33 mg g−1 of isoorientin, orientin and isovitexin, respectively) and was considered the best method according to the green and sustainable described factors.
Assessing the role of two populations of Aedes japonicus japonicus for Zika virus transmission under a constant and a fluctuating temperature regime
Authors: Glavinic Uros , Varga Jasmin , Paslaru Anca Ioana , Hauri Jeannine , Torgerson Paul , Schaffner Francis , Veronesi Eva 
Published: September 2020

AbstractBackgroundSince the huge epidemic of Zika virus (ZIKV) in Brazil in 2015, questions were raised to understand which mosquito species could transmit the virus.Aedes aegyptihas been described as the main vector. However, otherAedesspecies (e.g.Ae. albopictusandAe. japonicus) proven to be competent for other flaviviruses (e.g. West Nile, dengue and yellow fever), have been described as potential vectors for ZIKV under laboratory conditions. One of these, the Asian bush mosquito,Ae. japonicus, is widely distributed with high abundances in central-western Europe. In the present study, infection, dissemination and transmission rates of ZIKV (Dak84 strain) in two populations ofAe. japonicusfrom Switzerland (Zürich) and France (Steinbach, Haut-Rhin) were investigated under constant (27 °C) and fluctuating (14–27 °C, mean 23 °C) temperature regimes.ResultsThe two populations were each able to transmit ZIKV under both temperature regimes. Infectious virus particles were detected in the saliva of females from both populations, regardless of the incubation temperature regime, from 7 days post-exposure to infectious rabbit blood. The highest amount of plaque forming units (PFU) (400/ml) were recorded 14 days post-oral infection in the Swiss population incubated at a constant temperature. No difference in terms of infection, dissemination and transmission rate were found between mosquito populations. Temperature had no effect on infection rate but the fluctuating temperature regime resulted in higher dissemination rates compared to constant temperature, regardless of the population. Finally, transmission efficiency ranged between 7–23% and 7–10% for the constant temperature and 0–10% and 3–27% under fluctuating temperatures for the Swiss and the French populations, respectively.ConclusionsTo the best of our knowledge, this is the first study confirming vector competence for ZIKV ofAe. japonicusoriginating from Switzerland and France at realistic summer temperatures under laboratory conditions. Considering the continuous spread of this species in the northern part of Europe and its adaptation at cooler temperatures, preventative control measures should be adopted to prevent possible ZIKV epidemics.
Egg white hydrolyzate reduces mental fatigue: randomized, double-blind, controlled study
Authors: Oe Mariko , Sakamoto Hisae , Nishiyama Hiroshi , Sasahara Ryou , Masuda Yasunobu , Adachi Mizuho , Nishiyama Tetsunari 
Published: September 2020

Abstract
Objectives
This study aimed to show that ingesting egg white hydrolyzate (EWH) could improve antioxidant capacity and reduce mental fatigue. Two clinical trials were conducted to examine the antioxidant capacity and the fatigue reduction function of EWH. In Study 1, 19 athlete students were received a single dose of EWH (5 g/day) or placebo. In Study 2, 74 athlete students ingested EWH (5 g/day) or placebo before training for 2 weeks.

Results
Single dose of EWH significantly increased the antioxidant ability compared with the placebo group (p < 0.05), and there was no significant difference between the groups in the oxidative stress test results on Study 1. Two-week intake of EWH significantly decreased mental fatigue compared with the placebo (p < 0.05). This study showed that ingesting EWH improved antioxidant capacity with a single dose and reduced mental fatigue after 2 weeks of ingestion.
Trial Registration Japan Medical Association Center for Clinical Trials identifier; JMA-IIA00395 (Study1) and JMA-IIA00396 (Study2), both trials were retrospectively registered on 26 October, 2018.
GMP-grade neural progenitor derivation and differentiation from clinical-grade human embryonic stem cells
Authors: Vitillo Loriana , Durance Catherine , Hewitt Zoe , Moore Harry , Smith Austin , Vallier Ludovic 
Published: September 2020

Abstract
Background
A major challenge for the clinical use of human pluripotent stem cells is the development of safe, robust and controlled differentiation protocols. Adaptation of research protocols using reagents designated as research-only to those which are suitable for clinical use, often referred to as good manufacturing practice (GMP) reagents, is a crucial and laborious step in the translational pipeline. However, published protocols to assist this process remain very limited.

Methods
We adapted research-grade protocols for the derivation and differentiation of long-term neuroepithelial stem cell progenitors (lt-NES) to GMP-grade reagents and factors suitable for clinical applications. We screened the robustness of the protocol with six clinical-grade hESC lines deposited in the UK Stem Cell Bank.

Results
Here, we present a new GMP-compliant protocol to derive lt-NES, which are multipotent, bankable and karyotypically stable. This protocol resulted in robust and reproducible differentiation of several clinical-grade embryonic stem cells from which we derived lt-NES. Furthermore, GMP-derived lt-NES demonstrated a high neurogenic potential while retaining the ability to be redirected to several neuronal sub-types.

Conclusions
Overall, we report the feasibility of derivation and differentiation of clinical-grade embryonic stem cell lines into lt-NES under GMP-compliant conditions. Our protocols could be used as a flexible tool to speed up translation-to-clinic of pluripotent stem cells for a variety of neurological therapies or regenerative medicine studies.
Fractional inclusions of the Hermite–Hadamard type for m-polynomial convex interval-valued functions
Authors: Nwaeze Eze R. , Khan Muhammad Adil , Chu Yu-Ming 
Published: September 2020

AbstractThe notion of m-polynomial convex interval-valued function $\Psi =[\psi ^{-}, \psi ^{+}]$
Ψ
=
[

ψ
−

,

ψ
+

]
is hereby proposed. We point out a relationship that exists between Ψ and its component real-valued functions $\psi ^{-}$

ψ
−

and $\psi ^{+}$

ψ
+

. For this class of functions, we establish loads of new set inclusions of the Hermite–Hadamard type involving the ρ-Riemann–Liouville fractional integral operators. In particular, we prove, among other things, that if a set-valued function Ψ defined on a convex set S is m-polynomial convex, $\rho,\epsilon >0$
ρ
,
ϵ
>
0
and $\zeta,\eta \in {\mathbf{S}}$
ζ
,
η
∈
S
, then $$\begin{aligned} \frac{m}{m+2^{-m}-1}\Psi \biggl(\frac{\zeta +\eta }{2} \biggr)& \supseteq \frac{\Gamma _{\rho }(\epsilon +\rho )}{(\eta -\zeta )^{\frac{\epsilon }{\rho }}} \bigl[{_{\rho }{\mathcal{J}}}_{\zeta ^{+}}^{\epsilon } \Psi (\eta )+_{ \rho }{\mathcal{J}}_{\eta ^{-}}^{\epsilon }\Psi (\zeta ) \bigr] \\ & \supseteq \frac{\Psi (\zeta )+\Psi (\eta )}{m}\sum_{p=1}^{m}S_{p}( \epsilon;\rho ), \end{aligned}$$

m

m
+

2

−
m

−
1

Ψ

(

ζ
+
η

2

)

⊇

Γ
ρ

(
ϵ
+
ρ
)

(
η
−
ζ
)

ϵ
ρ

[
ρ

J

ζ
+

ϵ

Ψ
(
η
)

+
ρ

J

η
−

ϵ

Ψ
(
ζ
)
]

⊇

Ψ
(
ζ
)
+
Ψ
(
η
)

m

∑

p
=
1

m

S
p

(
ϵ
;
ρ
)
,

where Ψ is Lebesgue integrable on $[\zeta,\eta ]$
[
ζ
,
η
]
, $S_{p}(\epsilon;\rho )=2-\frac{\epsilon }{\epsilon +\rho p}- \frac{\epsilon }{\rho }\mathcal{B} (\frac{\epsilon }{\rho }, p+1 )$

S
p

(
ϵ
;
ρ
)
=
2
−

ϵ

ϵ
+
ρ
p

−

ϵ
ρ

B
(

ϵ
ρ

,
p
+
1
)
and $\mathcal{B}$
B
is the beta function. We extend, generalize, and complement existing results in the literature. By taking $m\geq 2$
m
≥
2
, we derive loads of new and interesting inclusions. We hope that the idea and results obtained herein will be a catalyst towards further investigation.
Fishes can use axial muscles as anchors or motors for powerful suction feeding
Authors: Camp Ariel L. , Olsen Aaron M. , Hernandez L. Patricia , Brainerd Elizabeth L. 
Published: September 2020

ABSTRACT
Some fishes rely on large regions of the dorsal (epaxial) and ventral (hypaxial) body muscles to power suction feeding. Epaxial and hypaxial muscles are known to act as motors, powering rapid mouth expansion by shortening to elevate the neurocranium and retract the pectoral girdle, respectively. However, some species, like catfishes, use little cranial elevation. Are these fishes instead using the epaxial muscles to forcefully anchor the head, and if so, are they limited to lower-power strikes? We used X-ray imaging to measure epaxial and hypaxial length dynamics (fluoromicrometry) and associated skeletal motions (XROMM) during 24 suction feeding strikes from three channel catfish (Ictalurus punctatus). We also estimated the power required for suction feeding from oral pressure and dynamic endocast volume measurements. Cranial elevation relative to the body was small (&lt;5 deg) and the epaxial muscles did not shorten during peak expansion power. In contrast, the hypaxial muscles consistently shortened by 4–8% to rotate the pectoral girdle 6–11 deg relative to the body. Despite only the hypaxial muscles generating power, catfish strikes were similar in power to those of other species, such as largemouth bass (Micropterus salmoides), that use epaxial and hypaxial muscles to power mouth expansion. These results show that the epaxial muscles are not used as motors in catfish, but suggest they position and stabilize the cranium while the hypaxial muscles power mouth expansion ventrally. Thus, axial muscles can serve fundamentally different mechanical roles in generating and controlling cranial motion during suction feeding in fishes.
A rare case of Raoultella planticola urinary tract infection. A case report with literature review
Authors: Kozioł Magdalena , Lewicki Marcin , Pawlicki Mateusz , Łopuszyńska Anna , Krasa Aleksandra , Piekarska Ewa , Krasowska Danuta 
Published: September 2020

Urine infections represent 40-50% of all infections in hospitalized patients. The most common uropathogens are E.coli, S.saprophitycus, and less commonly Enterobacteriaceae, such as Proteus mirabilis and Klebsiella pneumoniae. The spectrum of microorganisms isolated in hospital conditions may be wider. Raoultella planticola is a rare cause of human infections, so far only few cases of ZUM with this etiology have been described.A 56-years-old patient, with a history of cardiac disease, with exacerbation of chronic kidney disease, was admitted to the Department of Internal Medicine. The reason for admission was general deterioration. Many abnormalities in laboratory tests were detected. The doctors' attention was focused on the significant bacteriuria. Moreover, the patient reported dysuria symptoms. Material for urine microbiological culture was taken and empirical antibiotic therapy with ciprofloxacin started. The obtained result of urine culture allowed to identify the bacteria by R. planticola. As a result of the applied treatment, the patient's condition improved.An increasing number of infections with R. planticolla etiology is observed. The bacterium may lead to infections in many systems. The group of patients at risk of the disease includes people with renal dysfunction, who are immunosuppressed. Infections of R. planticolla etiology are usually characterized by good antibiotic response and positive prognosis.
Assessment of the muscular strength of the global handgrip and physical activity in patients treated with renal replacement therapy (RRT) by hemodialysis
Authors: Turoń-Skrzypińska Agnieszka , Babkiewicz Damian , Nizio Ewa , Boćkowski Radosław , Pulwer Katarzyna , Tomska Natalia , Szylińska Aleksandra , Rył Aleksandra , Rotter Iwona Edyta 
Published: September 2020

Introduction
Chronic Kidney Disease (CKD) is a social problem. Hemodialysis is the most common method of renal replacement therapy. At the beginning of hemodialysis treatment, physical activity is reduced by 50-60%. The aim of the study was to compare physical activity, handgrip strength and selected anthropometric parameters, and to assess the relationship between handgrip strength, selected anthropometric parameters and the level of physical activity.
Material and methods
The study included 30 patients aged 65.92 ± 14.65 treated by hemodialysis. The study consisted of patients completing a proprietary survey questionnaire, assessing physical activity using the International Physical Activity Questionnaire (IPAQ), handgrip strength, and performing selected anthropometric measurements. The examinations were performed at baseline (E0) and after three months of hemodialysis (E3) treatment and the results were compared.
Results
There were no significant differences in the study group for physical activity and global handgrip strength at baseline and after three months of the study. There was no statistically significant differences beetwen physical activity and handgrip strength in study and control group.
Conclusions
Patients treated with renal replacement present less physical activity compared to people with normal kidney function. The BMI value and level of physical activity does not affect the handgrip strength in hemodialysis patients.
All-dielectric metasurface with multi-function in the near-infrared band
Authors: Zhang Xiaodong , Kong Depeng , Liu Sujuan , Wang Haiyan 
Published: September 2020

This paper presents an approach to design the all-dielectric metasurface with multi-function in the near-infrared range of 1.5–1.6 µm. Based on the geometric phase principle, the all-dielectric metasurface is composed of the Si nanopillar and the

S
i
O

2

substrate as an emitter unit distributed in a

21

×

21

array. Under the incidence of the circularly polarized light at 1550 nm, the metasurface works as a vortex-beam generator with high performance which generates the vortex beam with topological charges of

±

1

, and the mode purity of the vortex beam is 90.66%. Under the incidence of the linearly polarized light at 1550 nm, the metasurface also works as the azimuthally/radially polarized beam generator with high performance, and the purities of the azimuthally and the radially polarized beams are 92.52% and 91.02%, respectively. Moreover, the metasurface generates different output spots under the different incident lights which can be applied to optical encryption, and the metasurface with the phase gradient also can be used as the dual-channel encoder/decoder in optical communication. The simulated results are in good agreement with the theoretical derivation. The designed metasurface may become a potential candidate as a multi-function photon device in the integrated optical system in the future.
Cost-Effectiveness of 5-Aminosalicylate Therapy in Combination With Biologics or Tofacitinib in the Treatment of Ulcerative Colitis
Authors: Shaffer Seth R. , Huang Elbert , Patel Shivani , Rubin David T. 
Published: September 2020

INTRODUCTION:
Medications are major cost drivers in the treatment of patients with inflammatory bowel disease. Recent analyses suggest that there is no added efficacy in continuing nor harm in stopping 5-aminosalicylate (ASA) therapy in patients with inflammatory bowel disease escalated to biological therapies or tofacitinib. We assessed the cost-effectiveness of discontinuing 5-ASA therapy in patients with ulcerative colitis on biological therapies or tofacitinib, compared with continuing 5-ASA therapy.

METHODS:
We performed a cost-effectiveness analysis of 5-ASA with biologic therapy and tofacitinib compared with the same treatment without 5-ASA. Our primary outcome was to determine whether biologic/tofacitinib monotherapy was cost-effective compared with biologic/tofacitinib and 5-ASA combination therapy using the incremental cost-effectiveness ratio at a willingness to pay of $50,000/quality-adjusted life year. Owing to the uncertainty surrounding outcome probabilities, probabilistic sensitivity analyses with 10,000 simulations were also performed. We conducted a sensitivity analysis comparing biologic/tofacitinib and 5-ASA therapy compared with biologic/tofacitinib monotherapy, whereby vedolizumab was the first biologic used, followed by infliximab and finally tofacitinib.

RESULTS:
Our model shows that biologic/tofacitinib monotherapy dominates (cheaper and more effective) combination therapy of biologics/tofacitinib with 5-ASA. Probabilistic sensitivity analyses simulations resulted in biologic/tofacitinib monotherapy dominating 100% of the scenarios, with mean cost savings of $24,483.01 over 2 years. When vedolizumab was the first-line therapy in the sensitivity analysis, biologic/tofacitinib monotherapy continued to dominate the combination of 5-ASA and biologic/tofacitinib therapy.

DISCUSSION:
This analysis in patients with ulcerative colitis who require treatment with biologics or tofacitinib demonstrates that continuing 5-ASA therapy is not a cost-effective strategy. Discontinuation of 5-ASA therapy in these patients is safe and less expensive and should be recommended.
Gaming Musical Instruments
Authors: Torge Claussen Jan 
Published: September 2020

Abstract
This article addresses the relationship between labour and learning a popular musical instrument like the guitar in the specific context of a video game. Most gamification theories promise that using a video game makes it easy to learn (Kapp 2012; Deterding et al. 2011). Even if this holds true, I argue that this kind of playfulness causes some backlash, which I observed during an experiment in which students played the music video game Rocksmith 2014. Learning and playing the guitar through the medium of a video game comes with diverse experiences as well as expectations that are closely related to the dichotomies between play and work, often discussed in game studies based on the famous texts by Johann Huizinga (2004) and Roger Caillois (1960). Learning any traditional music instrument requires much effort in several skill areas, for example, dexterity, hearing, sight-reading, and performance. In other words, it seems to be hard work and not at all playful like a video game. In this article, the various aspects of playful work and labourious play, found in both music education and guitar games, will be discussed against the backdrop of empirical findings including data from online interviews, research diaries and video recordings.
Analisis Pengaruh Dimensi Kualitas Pelayanan terhadap Kepuasan Pasien Peserta BPJS Kesehatan di Instalasi Rawat Jalan RSUD dr. H. Soewondo Kendal
Authors: Widyastuti Nur , Widjanarko Bagoes , Adi Mateus Sakundarno 
Published: September 2020

Tujuan penelitian ini adalah untuk menganalisis apakah ada pengaruh antara dimensi kualitas pelayanan yaitu tangible, reliability, responsiveness, assurance, dan empathy terhadap kepuasan pasien peserta BPJS Kesehatan di instalasi rawat jalan RSUD dr. H. Soewondo Kendal.Penelitian ini menggunakan metode pendekatan kuantitatif dengan desain cross sectional . Populasi penelitian adalah pasien rawat jalan peserta BPJS Kesehatan di poli rawat jalan RSUD dr. H. Soewondo Kendal, sedangkan subyeknya adalah pasien peserta BPJS Kesehatan di poli rawat jalan yang telah pernah periksa minimal 2-3 kali dengan menggunakan kartu BPJS Kesehatan. Pengumpulan data menggunakan cara Non Probability Sampling. Data diolah dan dianalisisi dengan analisis Smart PLS. Besar sampel sejumlah 100 orang responden dan menggunakan teknik Purposive Sampling untuk pengambilan sampel. Berdasarkan analisis dengan Smart PLS hasil penelitian menunjukkan bahwa tangible / bentuk fisik mempunyai pengaruh positif secara signifikan terhadap kepuasan pasien dengan nilai t-hitung 3,043 > 1.96 , reliability / keandalan mempunyai pengaruh positif secara signifikan terhadap kepuasan pasien dengan nilai t-hitung 2,571 > 1.96, responsiveness / daya tanggap mempunyai pengaruh positif secara signifikan terhadap kepuasan pasien dengan nilai t-hitung 3,101 > 1.96, assurance / jaminan tidak mempunyai pengaruh positif secara signifikan terhadap kepuasan pasien dengan nilai t-hitung 1,491 < 1.96, dan empathy / perhatian mempunyai pengaruh positif secara signifikan terhadap kepuasan pasien dengan nilai t-hitung 3,469 > 1.96. Hasil uji hipotesis ini menunjukkan bahwa tidak semua dimensi kualitas pelayanan berpengaruh terhadap kepuasan pasien peserta BPJS Kesehatan.Kesimpulan penelitian ini adalah empat dari lima dimensi kualitas pelayanan yang diteliti mempunyai pengaruh positip secara signifikan terhadap kepuasan pasien peserta BPJS Kesehatan di Instalasi Rawat Jalan RSUD dr. H. Soewondo. Dimensi mutu tangible, reliability, responsiveness dan emphaty mempunyai pengaruh terhadap kepuasan pasien peserta BPJS Kesehatan di Instalasi Rawat Jalan RSUD dr. H. Soewondo dan dimensi assurance tidak mempunyai pengaruh terhadap kepuasan pasien.