bluuebunny/crossref_metadata_2025_split huggingface dataset:
A microbead-enhanced electrochemical platform for β-amyloid peptide (1–42) detection
Authors: Razzino Claudia do Amaral , Sgobbi Lívia Flório , Cancino-Bernardi Juliana , Zapata Angelica Maria Mazuera , Costa Clara Cardoso , Zucolotto Valtencir , Vieira Lucia , Lobo Anderson Oliveira 
Published: December 2024

Alzheimer’s disease is the most prevalent form of dementia and is primarily characterized by the accumulation of β-amyloid and phosphorylated tau proteins in the brain, along with the degeneration of nerve cells, which leads to impairment of various cognitive functions. A significant biomarker of Alzheimer’s disease is the decreased level of soluble β-amyloid peptide (1–42) (Aβ1-42) in cerebrospinal fluid (CSF), as pathology progresses when CSF-Aβ1-42 levels drop below 192 pg mL−1. In this study, we developed an amperometric immunosensor based on magnetic beads as the platform for constructing the immunosensor. Monoclonal antibodies are immobilized on the MBs, enabling selective detection of Aβ1-42. The detection antibody is conjugated with the enzyme horseradish peroxidase, which, in the presence of H2O2 and hydroquinone, catalyzes the decomposition of H2O2 and the oxidation of hydroquinone to p-quinone, generating an electric current measured at a potential of −200 mV (vs. the Ag pseudo-reference electrode) using screen-printed carbon electrodes. The amperometric sandwich-type immunosensor demonstrates a linear response in the concentration range of 10 to 10,000 pg mL−1, with a detection limit of 7.4 pg mL−1, exhibiting excellent selectivity against the assessed interferents. These findings suggest the potential application of this immunosensor in the early diagnosis of Alzheimer’s disease, offering a sensitive and specific tool for clinical analysis. Despite its high performance, further studies are required to validate its robustness and applicability in complex clinical samples.
Finance-driven sustainable development: the impact of green finance on agricultural non-point source pollution and its pathways
Authors: Shen Yang , Zhang Xiuwu 
Published: December 2024

IntroductionPollutants from farming and rural life have a serious negative impact on water and soil. The advantages of green finance in financial support and credit management can guide the green transformation of agricultural production, thus protecting the ecological environment.MethodsBased on panel data from 30 provinces in China from 2005 to 2021, the study used inventory analysis, entropy method and two-way fixed effect model to analyze the impact of green finance on agricultural non-point source pollution (ANSP) and the mediating mechanism.ResultsANSP has experienced an inverted U-shaped trend of first rising and then falling, and the rural ecological environment is improving. At the time level, the ANSP produced by agricultural production in China experienced an inverted U-shaped evolution trend of first increasing and then decreasing during the sample period. At the spatial level, ANSP has a significant low regional differentiation. The ANSP of the central region and the coastal economic development area is higher than that of the western region. The results of linear regression show that green finance has a negative effect on ANSP. Promoting green finance in rural areas can effectively guide the green transformation of agriculture and reduce the dependence of crop cultivation on fertilizers and pesticides. This result held even after the elimination of endogeneity and after various tests. Land transfer and environmental supervision are important intermediary mechanisms. Under the influence of these two economic variables, the role of green finance in pollution reduction can be fully brought into play. However, there is regional heterogeneity in the negative effects of green finance on ANSPs. Its effect on pollution reduction is stronger in major grain-producing areas and regions with well-developed digital financial infrastructure.DiscussionThis study confirms once again that green finance has a negative effect on ANSP, and the findings help to decouple the development of the agricultural economy from emissions. The outstanding contribution of this study is the discovery of the role of land transfer and government environmental regulation. I order to give full play to the role of green finance in pollution reduction, this study provides relevant policy measures. These measures are intended to improve the green finance system and business model in agriculture and rural areas.
Use of intrauterine dextrose as an alternative to systemic antibiotics for treatment of clinical metritis in dairy cattle: a microbiome perspective
Authors: Lection Jennine , Van Syoc Emily , Miles Asha , Hamilton Julia , Martinez Marcela , Bas Santiago , Silverman Justin , Barragan Adrian , Ganda Erika 
Published: December 2024

IntroductionClinical metritis (CM) has significant costs to dairy producers. Current treatment strategy involves systemic antibiotics; however, there is increasing concern about judicious antibiotic use. The study objective was to evaluate the effects of a non-antibiotic treatment vs. systemic antibiotic therapy on the vaginal discharge microbiome of dairy cows diagnosed with CM at 7 ± 3 DIM (days in milk). We hypothesize that both treatment methods will have a similar impact on the reproductive microbiome due to broad-spectrum bactericidal activity; therefore, there will not be significant differences amongst the microbiota after the completion of therapy.MethodsCows from a central Pennsylvania dairy were screened for CM at 7 ± 3 days DIM using a Metricheck™ device (n = 351). Cows with red-brown watery discharge were diagnosed with CM and eligible for enrollment. Eligible cows (n = 77) were blocked by parity and randomly allocated to one of two treatments starting on the day of diagnosis: (1) Intrauterine dextrose (DEX, n = 38): 1 l of an intrauterine 50% dextrose solution for 3 days, and (2) Systemic ceftiofur (CONV, n = 39): two injections of ceftiofur (6.6 mg/Kg of BW; Excede, Zoetis Inc.) 72 h apart. Cows were evaluated for clinical cure rate at 7 ± 3 and 14 ± 3 days post-diagnosis. Vaginal discharge samples were collected using the Metricheck™ at enrollment day [study day (sd) 0, pre-treatment], sd 7, and sd 14 for a subset of enrolled cows (DEX = 13, CONV = 14). Vaginal discharge samples were analyzed with 16S rRNA sequencing to evaluate changes in the microbiome between treatments.ResultsAfter treatment, there were only minor differences within the microbiome between the two treatments indicating the potential suitability of dextrose as an antibiotic-alternative treatment. Alpha diversity did not differ (Welch's t-test) between the treatments at any of the time points. Beta diversity based on PERMANOVA analysis did differ between treatments at sd 0 (P = 0.014) and again at sd 14 (P = 0.028), but not at sd 7 (P = 0.261).DiscussionWhile 16S rRNA analysis does not provide information on bacterial viability, the relative similarity of the microbiome between the two groups immediately following treatment might suggest that intrauterine dextrose could be utilized as an alternative treatment for CM.
Novel coronaviruses and mammarenaviruses of hedgehogs from Russia including the comparison of viral communities of hibernating and active specimens
Authors: Lukina-Gronskaya A. V. , Chudinov I. K. , Korneenko E. V. , Mashkova S. D. , Semashko T. A. , Sinkova M. A. , Penkin L. N. , Litvinova E. M. , Feoktistova N. Yu , Speranskaya A. S. 
Published: December 2024

IntroductionSmall mammals, especially rodents and bats, are known reservoirs of zoonotic viruses, but little is known about the viromes of insectivorous species including hedgehogs (order Eulipotyphla), which often live near human settlements and come into contact with humans.MethodsWe used high-throughput sequencing and metaviromic analysis to describe the viromes of 21 hedgehogs (Erinaceus sp.) sampled from summer 2022 to spring 2023. We captured 14 active animals from the wild (seven in European Russia and the other seven in Central Siberia). The remaining 7 animals were hibernating in captivity (captured in European Russia before the experiment).Results and discussionThe diversity of identified viral taxa as well as the total number of reads classified as viral was high in all active animals (up to eight different viral families per animal), but significantly lower in hibernating animals (zero or no more than three different viral families per animal). The present study reports, for the first time, betacoronaviruses and mammasrenaviruses in hedgehogs from Russia. Erinaceus coronaviruses (EriCoVs) were found in 4 of 7 active animals captured in the wild, in European Russia, making it is the easiest finding of EriCoVs in Europe. One animal was found to carry of two different EriCoVs. Both strains belong to the same phylogenetic clade as other coronaviruses from European hedgehogs. Pairwise comparative analysis suggested that one of these two strains arose by recombination with an unknown coronavirus, since all of identified SNPs (n = 288) were found only in the local genome region (the part of ORF1b and S gene). The novel mammarenaviruses (EriAreVs) were detected in 2 out of 7 active and in 2 out of 7 hibernating animals from the European Russia. Several complete L and S segments of EriAreVs were assembled. All identified EriAreVs belonged to the same clade as the recently described MEMV virus from Hungarian hedgehogs. As the hibernating hedgehogs were positive for EriAreVs when kept in controlled conditions without contact with each other, we suggest the possibility of persistent arenavirus infection in hedgehogs, but further experiments are needed to prove this.
The Flip Side of the Coin: Giftedness in Pediatric Acute-Onset Neuropsychiatric Syndrome
Authors: Calaprice Denise , Terreri Ryan , Whitty Christopher , Whitty Ryan , Tona Janice 
Published: December 2024

Background/Objectives: Individuals with Pediatric Acute-onset Neuropsychiatric Syndrome (PANS), an immune-modulated disorder, experience exacerbation-related neuropsychiatric symptoms, functional impairments, and high rates of developmental diagnosis. The literature describes links between giftedness and mental illness, and giftedness and autoimmune disorders. We sought to explore rates of giftedness among children with PANS as perceived by their caregivers, and to examine whether giftedness was related to PANS symptom severity, persistence, or duration. Methods: Data were extracted from a larger, 146-item survey, with 680 respondents meeting inclusion criteria of being a parent/guardian of a child with PANS and answering questions regarding perceived giftedness in empathy, social skills, verbal ability, reading, memory, math, creativity, or “other.” Results: In all, 604 respondents indicated some type of giftedness; the categories of giftedness were each endorsed by 30–57% of respondents. We found no significant associations between giftedness and severity of worst symptoms, persistence of symptoms, or length of time since symptom onset, once Bonferonni corrections were applied. Significantly more females than males were identified as gifted in creativity, but no other sex-related differences were seen. Thematic analysis of optional comments revealed three themes: (1) Elaboration on Types of Giftedness; (2) Objective Basis for Perceptions of Giftedness; and (3) Impact of PANS on Giftedness. Conclusions: The rate of giftedness reported by parents of PANS subjects in this study is much higher than would be expected in the general population, even when adjusting generously for potential overestimation. This study of the “flip side” of PANS should serve as impetus for future studies regarding giftedness in this population; a robust finding of exceptionally high rates of giftedness would have implications for diagnosis, interpretation of symptoms (for example, perfectionism and social challenges) and disease management.
Biotic Factors Affecting Elm Health in Ukraine
Authors: Meshkova Valentyna , Kuznetsova Olena , Borysenko Oleksandr , Korsovetskyi Volodymyr , Pyvovar Tetiana 
Published: December 2024

Elms (Ulmus spp.) are widely spread in the forest, shelter belts, and urban landscaping. This research aimed to reveal the trends of Ulmus sp. health in Ukraine under biotic damage. The tasks included: (i) analyzing the presence of Ulmus sp. in the forests; (ii) studying the dynamics of Ulmus sp. health for 2001–2015 in the monitoring plots in the frame of the International Cooperative Programme on Assessment and Monitoring of Air Pollution Effects on Forests (ICP-Forests); (iii) assessing the prevalence of the dominant biotic factors affecting elm health and the probability of tree death or recovery. As a result of research, elms were found in 3.58% of the area in the stands with other main forest-forming species in the forests subordinated to the State Specialized Forest Enterprise «Forests of Ukraine». Four elm species are present in the forests of all regions of Ukraine. In the Forest zone, U. minor predominates, U. glabra is more common in the western part of the country, and U. pumila in the southern and eastern regions. In the ICP-Forests monitoring plots for 2001–2015, a trend of elm deterioration in 2007–2012 was found. The highest incidence of trees with disease symptoms was recorded for U. pumila. In the sample plots for 2023–2024, the health of three elm species tended to deteriorate. In 2024, mortality occurred among all elm species with symptoms of Dutch elm disease (DED) and among U. pumila trees with symptoms of wetwood. However, several trees have recovered. The results show the gaps in our knowledge that need to be filled, particularly in identifying resistant individuals and using their progeny to create resistant stands.
Identification, Phylogeny, and Expression Profiling of Pineapple Heat Shock Proteins (HSP70) Under Various Abiotic Stresses
Authors: Xu Rui , Wei Fangjun , Chen Yanzhao , Khan Faiza Shafique , Wei Yongzan , Zhang Hongna 
Published: December 2024

Pineapple (Ananas comosus (L.) Merr.) is an economically significant and delicious tropical fruit. Pineapple commercial production faces severe decline due to abiotic stresses, which affect the development and quality of pineapple fruit. Heat shock protein 70 (HSP70) plays an essential role in abiotic stress tolerance. However, the pineapple HSP70 family identification and expression analysis in response to abiotic stresses has not been studied. To explore the functional role of AcHSP70, different abiotic stress treatments were applied to pineapple cultivar “Bali” seedlings. A total of 21 AcHSP70 members were identified in the pineapple genome. The identified genes were classified into four subfamilies (I–IV) using phylogenetic analysis. The AcHSP70 family is expressed under different stress conditions. Quantitative real time polymerase chain reaction (qRT-PCR) revealed the expression pattern of the AcHSP70 family under cold, drought, salt, and heat stress. The expression level of genes such as AcHSP70-2 increased under heat, cold, and drought stress, while the expression level of genes such as AcHSP70-3 decreased under salt stress. Furthermore, the expression profile of AcHSP70s in different tissues and development stages was analyzed using transcriptome analysis. The HSP70 genes exhibited unique expression patterns in pineapple tissue at different developmental stages. The study therefore provides a list of HSP70 genes with substantial roles in abiotic stress response and valuable information for understanding AcHSP70 functional characteristics during abiotic stress tolerance in pineapple.
Chemical Diversity of Mediterranean Seagrasses Volatilome
Authors: Coquin Salomé , Ormeno Elena , Pasqualini Vanina , Monnier Briac , Culioli Gérald , Lecareux Caroline , Fernandez Catherine , Saunier Amélie 
Published: December 2024

Background/Objectives: Biogenic volatile organic compounds (BVOCs), extensively studied in terrestrial plants with global emissions around 1 PgC yr−1, are also produced by marine organisms. However, benthic species, especially seagrasses, are understudied despite their global distribution (177,000–600,000 km2). This study aims to examine BVOC emissions from key Mediterranean seagrass species (Cymodocea nodosa, Posidonia oceanica, Zostera noltei, and Zostera marina) in marine and coastal lagoon environments. Methods: BVOCs were collected using headspace solid-phase microextraction (HS-SPME) using divinylbenzene/carboxen/polydimethylsiloxane (DVB/CAR/PDMS) fibers and analyzed by gas chromatography–mass spectrometry (GC-MS). Results: An important chemical diversity was found with a total of 92 volatile compounds (61 for Z. noltei, 59 for C. nodosa, 55 for P. oceanica, and 51 for Z. marina), from different biosynthetic pathways (e.g., terpenoids, benzenoids, and fatty acid derivatives) and with several types of chemical functions (e.g., alkanes, esters, aldehydes, and ketones) or heteroatoms (e.g., sulfur). No differences in chemical richness or diversity of compounds were observed between species. The four species shared 29 compounds enabling us to establish a specific chemical footprint for Mediterranean marine plants, including compounds like benzaldehyde, benzeneacetaldehyde, 8-heptadecene, heneicosane, heptadecane, nonadecane, octadecane, pentadecane, tetradecane, and tridecanal. PLS-DA and Heatmap show that the four species presented significantly different chemical profiles. The major compounds per species in relative abundance were isopropyl myristate for C. nodosa (25.6%), DMS for P. oceanica (39.3%), pentadecane for Z. marina (42.9%), and heptadecane for Z. noltei (46%). Conclusions: These results highlight the potential of BVOCs’ emission from seagrass ecosystems and reveal species-specific chemical markers.
Flyways of Common Cranes Grus grus breeding in Fennoscandia
Authors: Hansson Per , Nilsson Lovisa , Lundgren Sigvard , Skyllberg Ulf , Sandvik Jostein , Månsson Johan 
Published: December 2024

Knowledge of migration patterns plays an essential role for understanding spatiotemporal distribution of birds. Here we used >15,000 sightings of 1,473 colour-ringed Common Cranes Grus grus to study migration patterns of birds breeding in five regions along a longitudinal gradient of Fennoscandia. Our results confirm that Fennoscandian cranes mainly use either a Western European flyway (W-flyway), to winter mainly in France or Spain, or a Central European flyway (C-flyway), to winter in Hungary or Israel. Finnish cranes showed the greatest variation in migration patterns in terms of distance and direction and only Finnish cranes were recovered in Africa. Many of the Finnish cranes, starting along C-flyway change to the W-flyway and winter in SW Europe or NW Africa. On the other hand, the Scandinavian cranes are rarely observed along the C-flyway. However, substantial numbers of cranes from NE Sweden cross the Baltic Sea and migrate via Finland but then follow the W-flyway from Germany and southwards, especially during autumn. These results can be used for conservation and management, e.g. to coordinate monitoring. They are also relevant for land use planning, e.g. to avoid windfarms at sites important for migrating soaring birds while crossing seas, so-called ‘thermal bottleneck sites’.
УРОЖАЙНОСТЬ ОЗИМОЙ МЯГКОЙ ПШЕНИЦЫ И ЭЛЕМЕНТЫ СТРУКТУРЫ В СУХОСТЕПНОЙ ЗОНЕ ВОЛГОГРАДСКОЙ ОБЛАСТИ
Authors: Питоня А.А. , Питоня В.Н. 
Published: December 2024

В статье представлены результаты исследова- ний по выявлению наиболее значимых элементов структуры урожая, определяющих урожайность озимой мягкой пшеницы в специфических клима- тических условиях Волгоградской области, для ис- пользования в селекционном процессе на повыше- ние продуктивности и адаптивности новых сортов. В среднем за период с 2000 по 2018 годы установ- лена тесная корреляционная зависимость урожая от высоты растений (r0,9), массы зерна главного колоса (r0,69), массы 1000 зерен (r0,72) и ко- личества продуктивных колосьев на единице пло- щади (r0,73). При благоприятных погодных условиях зависи- мость урожая от элементов его структуры снижает- ся до средних значений. Коэффициент корреляции урожая следующий: от массы зерна главного колоса r 0,61 от массы 1000 зерен r0,52 от количест- ва продуктивных колосьев на 1м2 r0,57. При неблагоприятных погодных условиях на- блюдалась тесная корреляционная зависимость только от высоты растений (r 0,71) и средняя от массы зерна главного колоса (r 0,39) от количе- ства зерен в главном колосе r 0,48 от массы зер- на боковых побегов r 0,60 от массы 1000 зерен r0,54. Установлено, что первоначально отбор элитных растений следует проводить в стрессовых погод- ных условиях, повторно в благоприятных.
The article presents the results of research aimed to identify the most significant elements of the yield structure that determine the yield of winter soft wheat in specific climatic conditions of the Volgograd region, for use in the breeding process to increase the productivity and adaptability of new varieties. Close correlation dependence of yield on plant height (r 0.9), grain mass of the main ear (r 0.69), mass of 1000 grains (r 0.72) and the number of productive ears per area unit (r 0.73) was established on average for the period from 2000 to 2018. In case of favorable weather conditions, the dependence of the yield on the elements of its structure is reduced to average values. The correlation coefficient of the crop is as follows: from the grain mass of the main ear r 0.61 from the mass of 1000 grains r0.52 from the number of productive ears per 1m2 r0.57. Under adverse weather conditions, a close correlation was observed only from the height of plants (r 0.71), and the average - from the grain mass of the main ear (r 0.39) from the number of grains in the main ear r 0.48 from the grain mass of lateral shoots r 0.60 from the mass of 1000 grains r 0.54. It is established that the initial selection of elite plants should be carried out under stressful weather conditions, and again - under favorable respectively.