bluuebunny/crossref_metadata_2025_split huggingface dataset:
Exploring the action of RGDV-gemcitabine on tumor metastasis, tumor growth and possible action pathway
Authors: Zhang Xiaoyi , Zhang Jinhuan , Liu Wenchao , Wang Yaonan , Wu Jianhui , Zhao Shurui , Zhao Ming , Peng Shiqi 
Published: September 2020

AbstractThe coupling of Arg-Gly-Asp-Val (RGDV) and gemcitabine led to a hypothesis that the conjugate (RGDV-gemcitabine) could inhibit tumor metastasis. To confirm this hypothesis the activities of RGDV-gemcitabine inhibiting tumor metastasis in vitro and in vivo were presented for the first time. AFM (atomic force microscopy) imaged that RGDV-gemcitabine was able to adhere onto the surface of serum-starved A549 cells, to block the extending of the pseudopodia. Thereby RGDV-gemcitabine was able to inhibit the invasion, migration and adhesion of serum-starved A549 cells in vitro. On C57BL/6 mouse model RGDV-gemcitabine dose dependently inhibited the metastasis of planted tumor towards the lung and the minimal dose was 0.084 µmol/kg/3 days. The decrease of serum TNF-α (tumor necrosis factor), IL-8 (interleukin-8), MMP-2 (matrix metalloprotein-2) and MMP-9 (matrix metalloprotein-9) of the treated C57BL/6 mice was correlated with the action pathway of RGDV-gemcitabine inhibiting the metastasis of the planted tumor towards lung.
3D imaging of proximal caries in posterior teeth using optical coherence tomography
Authors: Shimada Yasushi , Burrow Michael F. , Araki Kazuyuki , Zhou Yuan , Hosaka Keiichi , Sadr Alireza , Yoshiyama Masahiro , Miyazaki Takashi , Sumi Yasunori , Tagami Junji 
Published: September 2020

AbstractOptical coherence tomography (OCT) can create cross-sectional images of tooth without X-ray exposure. This study aimed to investigate the diagnostic accuracy of 3D imaging of OCT for proximal caries in posterior teeth. Thirty-six human molar teeth with 51 proximal surfaces visibly 6 intact, 16 slightly demineralized, and 29 distinct carious changes were mounted to take digital radiographs and 3D OCT images. The sensitivity, specificity and area under the receiver operating characteristic curve (AUC) for the diagnosis of enamel caries and dentin caries were calculated to quantify the diagnostic ability of 3D OCT in comparison with digital radiography. Diagnostic accuracy was evaluated by the agreement with histology using weighted Kappa. OCT showed significantly higher sensitivity, AUC and Kappa values than radiography. OCT can be a safer option for the diagnosis of proximal caries in posterior teeth that can be applied to the patients without X-ray exposure.
Oxidation modification of chitosan-based mesoporous carbon by soft template method and the adsorption and release properties of hydroxycamptothecin
Authors: Wang Xianshu , Lin Qian , Pan Hongyan , Jia Shuangzhu , Wu Hong , Shi Yongyong , Wang Zhuhua 
Published: September 2020

AbstractSpray drying and a direct carbonization technology were coupled to prepare nitrogen-doped mesoporous carbon nanoparticles (NMCs) using chitosan as a carbon source and nitrogen source precursor and a triblock amphiphilic copolymer (F127) as a soft template, then oxidative modification was performed by ammonium persulfate (APS) to prepare oxidized mesoporous carbon nanoparticles (O-NMCs). The pore structure, chemical composition and wettability of the mesoporous materials were studied before and after oxidative modification, the microscopic morphology, structure, composition and wetting performance of the mesoporous carbon were characterized by transmission electron microscopy (TEM), an X-ray diffractometer (XRD), N2 adsorption–desorption instrument, X-ray photoelectron spectroscopy (XPS), contact angle tests and other analyses, meanwhile influences of the mesoporous carbon material on adsorption and release performance of a poorly-soluble antitumor drug hydroxycamptothecin (HCPT) were investigated. It was demonstrated from results that the surface wettability of the oxidatively-modified mesoporous carbon material was improved, the contact angle of the mesoporous carbon materials was reduced from 133.4° to 58.2° and the saturated adsorption capacity of HCPT was 676.97 mg/g and 647.20 mg/g respectively. The dissolution rate of the raw material hydroxycamptothecin was improved due to the nanopore structure of the mesoporous carbon material, the dissolution rate of mesoporous carbon material-loaded hydroxycamptothecin was increased from 22.7% to respective 83.40% and 81.11%.
Assessing the need for pioglitazone in the treatment of patients with type 2 diabetes: a meta-analysis of its risks and benefits from prospective trials
Authors: Sinha Binayak , Ghosal Samit 
Published: September 2020

AbstractThe safety and usefulness of pioglitazone (Pio) is repeatedly called into question due to the contradictory information available about it. A meta-analysis and risk benefit assessment was conducted to address the various points of debate regarding Pio. Electronic database search (Cochrane library, Embase & PubMed) resulted in 10 citations eligible for this meta-analysis (prospective, randomised studies), which was conducted using CMA software version 3 (Biostat Inc., Englewood, NJ, USA). The meta-analysis was registered with PROSPERO (ID: CRD42019122403) and compared pioglitazone with a control (anti-hyperglycemic agents without pioglitazone) in patients with either established cardiovascular disease or having high cardiovascular risk. Sensitivity and subgroup analysis were conducted to differentiate the effect of Pio against active controls and placebo. The use of Pio compared to the control group that did not use Pio resulted in a 14% and 23% significant reduction in odds of major adverse cardiac events (MACE: MH-OR, 0.86; 95% CI 0.75–0.98), and stroke (MH-OR, 0.77; 95% CI 0.60–0.99), respectively. This reduction in stroke was not significant in comparison to placebo on subgroup analysis. However, Pio significantly increased odds of heart failure (HF) (MH-OR, 1.47; 95% CI 1.26–1.71) as well as hospitalization for heart failure (hHF) (MH-OR, 1.48; 95% CI 1.21–1.81). In addition, the use of Pio was associated with a significant increase in odds of fractures in women (MH-OR, 2.05; 95% CI 1.28–3.27) and anaemia (MH-OR, 2.56; 95% CI 1.55–4.21). Pio has no significant effect on bladder cancer nor macular oedema. Pio has salutary effects on MACE. The positive effects are completely offset by the harm they seem to cause by way of heart failure, fractures, and anaemia. Pio should therefore be reserved for treatment of T2D with high CV risk or established cardiovascular (CV) disease only in selected patients where other antidiabetics are precluded and not routinely.
Whey protein isolate inhibits hepatic FGF21 production, which precedes weight gain, hyperinsulinemia and hyperglycemia in mice fed a high-fat diet
Authors: Nonogaki Katsunori , Kaji Takao 
Published: September 2020

AbstractInsufficient expression of hepatic fibroblast growth factor 21 (FGF21) and stromal cell-derived factor 2 like 1 (Sdf2l1) reportedly leads to insulin resistance and hepatosteatosis in obesity and type 2 diabetes. On the other hand, increased expression of hepatic serotonin receptor 2a (htr2a) in diet-induced obesity contributes to hepatosteatosis. Here we show that increases in circulating FGF21 levels and expression of hepatic FGF21 preceded weight gain, hyperinsulinemia, and hyperglycemia in C57BLJ6 mice fed a high-fat diet. Expression of hepatic htr2a and Sdf2l1 increased in insulin-resistant mice fed a high-fat diet. Intake of whey protein isolate decreased plasma FGF21 levels and expression of hepatic FGF21 in mice fed either a high-fat diet or a chow diet, whereas it only suppressed the overexpression of hepatic Sdf2 and htr2a in insulin-resistant mice fed a high-fat diet. Moreover, intake of whey protein isolate decreased plasma serotonin levels in mice fed either a high-fat diet or a chow diet. Genetic inhibition of tryptophan hydroxylase 1 decreased hepatic FGF21 expression and plasma FGF21 levels in mice. These findings suggest that increased hepatic FGF21 production precedes diet-induced weight gain, hyperinsulinemia, and hyperglycemia, and that intake of whey protein isolate could inhibit hepatic FGF21 production by suppressing peripheral serotonin synthesis.
Lactobacillus spp. attenuate antibiotic-induced immune and microbiota dysregulation in honey bees
Authors: Daisley Brendan A. , Pitek Andrew P. , Chmiel John A. , Gibbons Shaeley , Chernyshova Anna M. , Al Kait F. , Faragalla Kyrillos M. , Burton Jeremy P. , Thompson Graham J. , Reid Gregor 
Published: September 2020

AbstractWidespread antibiotic usage in apiculture contributes substantially to the global dissemination of antimicrobial resistance and has the potential to negatively influence bacterial symbionts of honey bees (Apis mellifera). Here, we show that routine antibiotic administration with oxytetracycline selectively increased tetB (efflux pump resistance gene) abundance in the gut microbiota of adult workers while concurrently depleting several key symbionts known to regulate immune function and nutrient metabolism such as Frischella perrera and Lactobacillus Firm-5 strains. These microbial changes were functionally characterized by decreased capped brood counts (marker of hive nutritional status and productivity) and reduced antimicrobial capacity of adult hemolymph (indicator of immune competence). Importantly, combination therapy with three immunostimulatory Lactobacillus strains could mitigate antibiotic-associated microbiota dysbiosis and immune deficits in adult workers, as well as maximize the intended benefit of oxytetracycline by suppressing larval pathogen loads to near-undetectable levels. We conclude that microbial-based therapeutics may offer a simple but effective solution to reduce honey bee disease burden, environmental xenobiotic exposure, and spread of antimicrobial resistance.
Pathogenic LRRK2 requires secondary factors to induce cellular toxicity
Authors: Lobbestael Evy , Van den Haute Chris , Macchi Francesca , Taymans Jean-Marc , Baekelandt Veerle 
Published: September 2020

Abstract
Pathogenic mutations in the leucine-rich repeat kinase 2 (LRRK2) gene belong to the most common genetic causes of inherited Parkinson’s disease (PD) and variations in its locus increase the risk to develop sporadic PD. Extensive research efforts aimed at understanding how changes in the LRRK2 function result in molecular alterations that ultimately lead to PD. Cellular LRRK2-based models revealed several potential pathophysiological mechanisms including apoptotic cell death, LRRK2 protein accumulation and deficits in neurite outgrowth. However, highly variable outcomes between different cellular models have been reported. Here, we have investigated the effect of different experimental conditions, such as the use of different tags and gene transfer methods, in various cellular LRRK2 models. Readouts included cell death, sensitivity to oxidative stress, LRRK2 relocalization, α-synuclein aggregation and neurite outgrowth in cell culture, as well as neurite maintenance in vivo. We show that overexpression levels and/or the tag fused to LRRK2 affect the relocalization of LRRK2 to filamentous and skein-like structures. We found that overexpression of LRRK2 per se is not sufficient to induce cellular toxicity or to affect α-synuclein-induced toxicity and aggregate formation. Finally, neurite outgrowth/retraction experiments in cell lines and in vivo revealed that secondary, yet unknown, factors are required for the pathogenic LRRK2 effects on neurite length. Our findings stress the importance of technical and biological factors in LRRK2-induced cellular phenotypes and hence imply that conclusions based on these types of LRRK2-based assays should be interpreted with caution.
The dual nature of obesity in metabolic programming: quantity versus quality of adipose tissue
Authors: Viveiros Anissa , Oudit Gavin Y. 
Published: September 2020

AbstractThe global prevalence of obesity has been rising at an alarming rate, accompanied by an increase in both childhood and maternal obesity. The concept of metabolic programming is highly topical, and in this context, describes a predisposition of offspring of obese mothers to the development of obesity independent of environmental factors. Research published in this issue of Clinical Science conducted by Litzenburger and colleagues (Clin. Sci. (Lond.) (2020) 134, 921–939) have identified sex-dependent differences in metabolic programming and identify putative signaling pathways involved in the differential phenotype of adipose tissue between males and females. Delineating the distinction between metabolically healthy and unhealthy obesity is a topic of emerging interest, and the precise nature of adipocytes are key to pathogenesis, independent of adipose tissue volume.
Experiences of Hearing Loss and Audiological Rehabilitation for Older Adults With Comorbid Psychological Symptoms: A Qualitative Study
Authors: Laird Emma C. , Bennett Rebecca J. , Barr Caitlin M. , Bryant Christina A. 
Published: September 2020

Purpose
There is a well-established relationship between hearing loss and psychological symptoms. To ensure audiological rehabilitation is provided appropriately for older adults with comorbid psychological symptoms, a greater understanding of their preferences and experiences is needed. This study sought to understand experiences of hearing loss and audiological rehabilitation from the perspective of older adults with comorbid psychological symptoms (e.g., depression, anxiety, psychosis).

Design
A qualitative study using in-depth semistructured interviews was conducted with older adults who had attended audiological rehabilitation within the last year and scored above established cutoffs on measures of depression, anxiety, and psychosis. A thematic analysis generated themes that related to participants' experiences of hearing loss and audiological rehabilitation.

Results

Participants included 14 older adults (eight men and six women) with an average age of 70.5 years (
SD
= 4.45, range: 64–80) who received hearing aids or a cochlear implant. Three major themes emerged from the analysis of participant interviews.
“
The cumulative impact of hearing loss and psychological symptoms” theme describes the two-way, additive relationship between hearing ability and psychological symptoms. “The experience of loss throughout hearing loss and audiological rehabilitation” captures subjective losses, the impact they have, and how participants cope with them. In contrast, “The experience of gain throughout hearing loss and audiological rehabilitation” describes the participants' reported gains, their related impacts, and coping strategies.

Conclusions
The experiences of participants revealed that the presence of comorbid psychological symptoms can influence the experience of hearing loss and audiological rehabilitation. These findings have implications for how audiological rehabilitation is provided to ensure optimal outcomes for adults with hearing loss and comorbid psychological symptoms.

Supplemental Material

https://doi.org/10.23641/asha.12985955
Effective Velopharyngeal Ratio: A More Clinically Relevant Measure of Velopharyngeal Function
Authors: Haenssler Abigail E. , Fang Xiangming , Perry Jamie L. 
Published: September 2020

Purpose
Velopharyngeal (VP) ratios are commonly used to study normal VP anatomy and normal VP function. An effective VP (EVP) ratio may be a more appropriate indicator of normal parameters for speech. The aims of this study are to examine if the VP ratio is preserved across the age span or if it varies with changes in the VP portal and to analyze if the EVP ratio is more stable across the age span.

Method
Magnetic resonance imaging was used to analyze VP variables of 270 participants. For statistical analysis, the participants were divided into the following groups based on age: infants, children, adolescents, and adults. Analyses of variance and a Games–Howell post hoc test were used to compare variables between groups.

Results

There was a statistically significant difference (
p
< .05) in all measurements between the age groups. Pairwise comparisons reported statistically significant adjacent group differences (
p
< .05) for velar length, VP ratio, effective velar length, adenoid depth, and pharyngeal depth. No statistically significant differences between adjacent age groups were reported for the EVP ratio.

Conclusions
Results from this study report the EVP ratio was not statistically significant between adjacent age groups, whereas the VP ratio was statistically significant between adjacent age groups. This study suggests that the EVP ratio is more correlated to VP function than the VP ratio and provides a more stable and consistent ratio of VP function across the age span.
Measuring the atomic composition of planetary building blocks
Authors: McClure M. K. , Dominik C. , Kama M. 
Published: September 2020

Context. Volatile molecules are critical to terrestrial planetary habitability, yet they are difficult to observe directly where planets form at the midplanes of protoplanetary disks. It is unclear whether the inner ∼1 AU of disks are volatile-poor or if this region is resupplied with ice-rich dust from colder disk regions. Dust traps at radial pressure maxima bounding disk gaps can cut off the inner disk from these types of volatile reservoirs. However, the trap retention efficiency and atomic composition of trapped dust have not been measured.
Aims. We present a new technique to measure the absolute atomic abundances in the gas accreting onto T Tauri stars and infer the bulk atomic composition and distribution of midplane solids that have been retained in the disk around the young star TW Hya.
Methods. We identify near-infrared atomic line emission from gas-phase material inside the dust sublimation rim of TW Hya. Gaussian decomposition of the strongest H Paschen lines isolates the inner disk hydrogen emission. We measure several key elemental abundances, relative to hydrogen, using a chemical photoionization model and infer dust retention in the disk. With a 1D transport model, we determine approximate radial locations and retention efficiencies of dust traps for different elements.
Results. Volatile and refractory elements are depleted from TW Hya’s hot gas by factors of ∼102 and up to 105, respectively. The abundances of the trapped solids are consistent with a combination of primitive Solar System bodies. Dust traps beyond the CO and N2 snowline cumulatively sequester 96% of the total dust flux, while the trap at 2 AU, near the H2O snowline, retains 3%. The high depletions of Si, Mg, and Ca are explained by a third trap at 0.3 AU with >95% dust retention.
Conclusion. TW Hya sports a significant volatile reservoir rich in C- and N-ices in its outer submillimeter ring structure. However, unless the inner disk was enhanced in C by earlier radial transport, typical C destruction mechanisms and the lack of a C resupply should leave the terrestrial planet-forming region of TW Hya “dry” and carbon-poor. Any planets that form within the silicate dust trap at 0.3 AU could resemble Earth in terms of the degree of their volatile depletion.
High-resolution, 3D radiative transfer modelling
Authors: Nersesian Angelos , Viaene Sébastien , De Looze Ilse , Baes Maarten , Xilouris Emmanuel M. , Smith Matthew W. L. , Bianchi Simone , Casasola Viviana , Cassarà Letizia P. , Clark Christopher J. R. , Dobbels Wouter , Fritz Jacopo , Galliano Frédéric , Madden Suzanne C. , Mosenkov Aleksandr V. , Trčka Ana 
Published: September 2020

Context. Investigating the dust heating mechanisms in galaxies provides a deeper understanding of how the internal energy balance drives their evolution. Over the last decade radiative transfer simulations based on the Monte Carlo method have emphasised the role of the various stellar populations heating the diffuse dust. Beyond the expected heating through ongoing star formation, older stellar populations (≥8 Gyr) and even active galactic nuclei can both contribute energy to the infrared emission of diffuse dust.
Aims. In this particular study we examine how the radiation of an external heating source, such as the less massive galaxy NGC 5195 in the M 51 interacting system, could affect the heating of the diffuse dust of its parent galaxy NGC 5194, and vice versa. Our goal is to quantify the exchange of energy between the two galaxies by mapping the 3D distribution of their radiation field.
Methods. We used SKIRT, a state-of-the-art 3D Monte Carlo radiative transfer code, to construct the 3D model of the radiation field of M 51, following the methodology defined in the DustPedia framework. In the interest of modelling, the assumed centre-to-centre distance separation between the two galaxies is ∼10 kpc.
Results. Our model is able to reproduce the global spectral energy distribution of the system, and it matches the resolved optical and infrared images fairly well. In total, 40.7% of the intrinsic stellar radiation of the combined system is absorbed by dust. Furthermore, we quantify the contribution of the various dust heating sources in the system, and find that the young stellar population of NGC 5194 is the predominant dust-heating agent, with a global heating fraction of 71.2%. Another 23% is provided by the older stellar population of the same galaxy, while the remaining 5.8% has its origin in NGC 5195. Locally, we find that the regions of NGC 5194 closer to NGC 5195 are significantly affected by the radiation field of the latter, with the absorbed energy fraction rising up to 38%. The contribution of NGC 5195 remains under the percentage level in the outskirts of the disc of NGC 5194. This is the first time that the heating of the diffuse dust by a companion galaxy is quantified in a nearby interacting system.