bluuebunny/crossref_metadata_2025_split huggingface dataset:
PPE 59 immunoreactivity in tuberculosis patients: IgA immunodominance to that IgG and IgM
Authors: Mulinari Ana Carla de Paulo , Sardella Isabela Gama , Silva Vania Maria C. da , Carvalho Anna Cristina C. , Matteelli Alberto , Saad Maria Helena Féres 
Published: March 2020

Abstract
Background: The role of protein families PE/PPE remains relatively enlightening. The gene rv3429, coding the PPE59 protein, is one of the 12 members of the PPE subfamily, and it is absent in all BCG strains. Although elicits low T cell response, there are no clues for the ability to induce a humoral response. Methods: We cloned, expressed, and analyzed by ELISA IgG, IgM and IgA anti PPE59 in 212 sera. Of them, 69 were from tuberculosis patients’ residents in Italy (TBIT, 12 native and 67 immigrants), the remaining 133 are Brazilians citizens (BR). Pulmonary (pTBIT) or extrapulmonary (eTBIT) clinical forms were 54 or 25. The pTBBR were 52 and 81 non-TB patients, including 10 non-tuberculous mycobacteria infected (NTM). Results: Keeping the specificity at 97%, IgA sensitivity decreased from pTBBR (53%) to pTBIT (38%) and eTBIT (28%), with an overall sensitivity of 42.7% and moderate accuracy (61.8 %), while IgG showed significant lower (p<0.0001) performance, 21%, 3%, 0%, 9% and 37%, respectively, at specificity of 83%. Groups were not discriminate by IgM. False-positive NTM IgG was high. Combination 16kDa IgG, previously performed only on Bazilians’ sera, plus PPE 59 IgA results increased sensitivity to 71% at 87.4% specificity. The overall smear microscopy (SM) diagnosis was 70.8% and a combination of SM/IgA increased sensitivity to 74% (p=0.01), mainly among SM- cases. Among pTBBR, all these rapid tests, including SM, sensitivity reach 86.5% (p=0.001). Positive IgA polarization was significant in extensive lung disease (p=0.001) and alcohol users (p=0.04). Conclusion: Although PPE59 IgA independently has moderate accuracy on TB diagnosis, together with other biomarkers contributes to improving its detection. Moreover, the polarized reactivity deserves further investigation.
Pulmonary manifestations in children with systemic lupus erythematosus
Authors: Jiang Wujun , Dai Ge , Wang Ting , Ma Jie , Zhu Canhong , Yan Yongdong , Chen Zhengrong 
Published: March 2020

Abstract
Background Symptomatic pulmonary involvement in systemic lupus erythematosus (SLE) is commonly reported in children. However, few studies have assessed risk factors that predict pulmonary involvement in SLE.Methods This was a seven-year retrospective study involving 111 SLE patients. The demographic, clinical and laboratory data of the patients were collected. Logistic regression analysis was performed to identify different clinical characteristics and laboratory parameters associated with presence of high resolution computerized tomography(HRCT) chest abnormalities.Results Of the 111 patients with SLE, we identified 18 patients (16.2%) with pulmonary involvement. The most common HRCT findings were ground glass opacity, interlobular septal thickening, bilateral diffuse infiltrates and pleurisy/pleural effusion (55.6%, 50%, 50%, and 44.4%, respectively). The variables independently associated with pulmonary involvement were anti- ribonucleoprotein (RNP) antibody (OR:7.9; 95% CI: 1.1–54.6) and anti-neutrophil cytoplasmic antibodies (ANCA, OR: 73.6; 95% CI: 2.9-1894.7).Conclusions Lung involvement was frequent in SLE patients from Southeast China. Anti-RNP antibody and ANCA were significantly associated with abnormal HRCT findings.
Effect of Fecal Microbiota Transplant on Symptoms of Psychiatric Disorders: A Systematic Review
Authors: Meyyappan Arthi Chinna , Forth Evan , Wallace Caroline , Milev Roumen 
Published: March 2020

Abstract
Background: The Gut-Brain-Axis is a bidirectional signaling pathway between the gastrointestinal (GI) tract and the brain. The hundreds of trillions of microorganisms populating the gastrointestinal tract are thought to modulate this connection, and have far reaching effects on the immune system, central and autonomic nervous systems, and GI functioning. These interactions have also been linked to various psychiatric illnesses such as depression, anxiety, substance abuse, and eating disorders. It is hypothesized that techniques aimed at strengthening and repopulating the gut microbiome, such as Fecal Microbiota Transplant (FMT), may be useful in the prevention and treatment of psychiatric illnesses. Methods: A systematic search of five databases was conducted using key terms related to FMT and psychiatric illnesses. All results were then evaluated based on specific eligibility criteria. Results: Twenty-one studies met the eligibility criteria and were analysed for reported changes in mood and behavioural measures indicative of psychiatric wellbeing. The studies included were either entirely clinical (n=7), preclinical with human donors (n=7), or entirely preclinical (n=7). All studies found a decrease in depressive and anxiety-like symptoms and behaviours resulting from the transplantation of healthy microbiota. The inverse was also found, with the transmission of depressive and anxiety-like symptoms and behaviours resulting from the transplantation of microbiota from psychiatrically ill donors to healthy recipients. Conclusion: There appears to be strong evidence for the treatment and transmission of psychiatric illnesses through FMT. Further research with larger sample sizes and stronger scientific design is warranted in order to fully determine the efficacy and safety of this potential treatment.
Protective effects of PAMAM dendrimers against ANIT-induced cholestatic liver injury in mice
Authors: Zhang Chuang , Liu Xuelai , Ren Weirui , Liu Shaojie , Zhang Xiangjian , Yang Desheng , Li Suolin 
Published: March 2020

Abstract
Background:Cholestatic liver disease (CLD) is a common disease of infancy, threatening infants’ health seriously. However, there is no effective drug to treat this disease.It is urgently to develop a new drug to overcome it. Polyamide-amine (PAMAM) dendrimer is a hyperbranched nano polymer, which has been found that has an anti-inflammatory effect recently. For this study, we aim to explore the protective effect and mechanism of PAMAM on CLD.Methods:In this study, a mouse model of cholestatic liver injury was created using ANIT,and TNF-α was utilized to stimulate human hepatocytes to investigate the protective effect and mechanism of 4.5th generation carboxyl-terminated PAMAM dendrimers (G4.5-COOH) in vivo and in vitro.Liver serum biochemistry, inflammatory, oxidative stress(OxS), endoplasmic reticulum(ER) stress and apoptosis indicators were determined.In addition, the eﬀects of G4.5-COOH on PPAR-γ and PI3K/AKT/mTOR signaling pathway were studied. Data were analyzed by using one-way ANOVA. Results:We find the G4.5-COOH may inhibit TNF-α to damage hepatocytes and ameliorate the cholestatic liver injury. We also find that the protective effect of G4.5-COOH might be due to inhibition of P-Akt、P-mTOR expression by a mechanism that might be partly dependent on up-regulated functional expression of PPAR-γ.Conclusions:G4.5-COOH PAMAM dendrimer has a protective effect on CLD.
Inert CD30-positive extranodal NK/T cell lymphoma with large cell transformation: a case report
Authors: Wang Hui , Yu PengYi , Li Qing 
Published: March 2020

Abstract
Background Extranodal natural killer (NK)/T-Cell Lymphoma is a rare type of cytotoxic lymphoma associated with Epstein-Barr virus infection, highly invasive and relatively resistant to chemotherapy. Inert CD30-positive extranodal NK/T cell lymphoma with large cell transformation is very rare. Case presentation A married 55-year-old male was admitted to hospital in February 2017, because of “diagnosis of malignant lymphoma for about 12 years with high fever and a left neck mass present for about 3 weeks". The patient was diagnosed to NK/T Cell Lymphoma as early as 2006, repeated relapses in June 2010 and April 2013. The patient was biopsied again in February 2017, lymph node, about 2.5×2×1.3cm.The section was off-white and tender. Microscopic examinations showed a diffuse proliferation of small and atypical lymphoid cells, admixed with large lymphoid cells. Immunohistochemically, the tumor cells exhibited positivity for CD30, CD3, CD43, CD2, CD56, TIA, negativity for CD4, CD8, AE1/AE3, CD20, ALK, EMA staining. In situ hybridization for EBER was strongly positive in the specimens.Conclusions Herein, we report a case of CD30-positive extranodal NK/T Cell Lymphoma with large cell transformation, nasal type, in indolent course over a period of 12 years. The accurate diagnosis of NK/T-cell lymphoma with CD30 expression and large cell transformation is very important. The disease usually has a poor prognosis related to several factors, but the indolent behavior of the present case is more unusual. A long-term follow-up is suggested to be performed to inspect the progression for this tumor.
MEK / ERK inhibitor effectively impact generalized lymphatic anomaly (GLA) cells growth through EGFR / MEK /ERK signaling pathway
Authors: Du Wa , Ayyaswamy Sriram , Rath Smruti , Ge Yan , Nguyen Tommy , Margolin Judith F , Kearny Debra , Phung Thuy L 
Published: March 2020

Abstract
Background Generalized lymphatic anomaly is characterized by diffuse or multicentric proliferation of dilated lymphatic vessels resembling common lymphatic malformation. Studies on GLA are frequently hampered by a lack of appropriate models to test the effects of potential treatments or decipher the mechanism of pathology. Moreover, diverse phenotypes observed with GLA require a large number of samples to be analyzed to obtain statistically informative results. Due to the very limited experimental material, most of the research is restricted to single case report. Methods We first time used two-step endothelial cell isolation technique (step 1: single cells were first sorted with a-human CD31 magnetic beads; step 2: collected CD31 Pos cells from step1 were sorted with a-human PDPN magnetic beads) to generate two GLA-LEC cell lines, and purified normal-LEC from normal liver tissue in the same case. To characterize the aberrant phenotype of generalized lymphatic anomaly lymphatic endothelial cells (GLA-LEC#1, and GLA-LEC#2). We investigated GLA-LECs growth curve, cell cycle, apoptosis, and sprouting angiogenesis in vitro . Matrigel plug assay was applied in immunodeficient mice to monitor the GLA-LECs formed vasculature in vivo . Rapamycin and dual MEK / ERK inhibitor were tested to investigate the efficacy on inhibiting GLA-LEC proliferation and downstream signaling pathway. Results We have successfully purified GLA-LECs from GLA tissues with > 99% purity. These cells also expressed the lymphatic markers lymphatic vessel endothelial hyaluronan receptor (LYVE-1) and podoplanin (PDPN). GLA-LECs showed significantly higher proliferation rate compared to normal-LECs in both cases. Cell cycle analysis of cell distribution suggested that compared with normal-LECs, GLA-LECs showed increased proportion of cells in S phase and less G0/G1 phase. When GLA-LECs and normal-LECs apoptosis induced by serum deprivation, more Annexin V positive population of endothelial cells were observed in normal-LECs but not GLA-LECs. Hyper-activated epidermal growth-factor receptor (EGFR) signaling was observed in both cases of GLA-LECs, endogenously highly expression of EGF receptor and EGF induced phosphorylation of EGFR (phosphor Y1068) were found in both GLA cell lines. GLA-LECs are sensitive to both rapamycin and MEK / ERK dual inhibitor treatment. In vivo, by using Matrigel plug assay, we found both GLA-LECs and immortalized GLA-LEC (SV40) grew robust vessel-like structure. Conclusions In vitro , both GLA-LECs cell lines are highly proliferative as compared with normal-LECs. Rapamycin and dual MEK / ERK inhibitor dose-dependently inhibited GLA-LECs proliferation. In vivo , GLA-LECs showed angiogenic phenotype, and grew robust vessel-like structure in immunodeficient mice.
The Correlation Analysis of Caries Status Between Primary Molars and First Permanent Molars
Authors: Xu Liang , Liang Yuee , Chen Leyi , Lv Xiaolin , Liu Haixia , Zhao Youran , Xu Wenan 
Published: March 2020

Abstract
Background First Permanent Molars (PFMs) are the most critical teeth in the dental arc. Meanwhile they bear the highest risk for carious lesions. The aim of this study is to investigate the caries status of primary and permanent teeth among 8-9-year-old children in Guangzhou, and the correlation between the caries of primary molars and first permanent molars(FPMs).Methods 119 samples of children aged 8-9 were included. The scoring and evaluation were processed in accordance with International Caries Detection and Assessment System (ICDAS). Statistical analysis was performed using SPSS 20.0 software package by means of Pearson Chi-square test and Kendall’s correlation.Results Pearson Chi-Square test showed that the prevalence of dental caries of the first primary molars, second primary molars and primary molars had significant correlation with that of FPMs while Kendall’s correlation analysis showed that they had weak positive correlation. What’s more, the Pearson chi-square test showed that there was no significant correlation between the degree of dental caries of different primary molars and PFM.Conclusion Caries in primary molars, especially the second molar, are considered to be a risk indicator for the development of permanent molars in the future. However, regardless of the degree of primary molars caries, the susceptibility of FPMs caries should be aware of. And pit and fissure sealants for them should be carried out as soon as possible.
Dynamic changes and effects of environmental tobacco smoke exposure and weaning time on gut microbiota in young children aged 0-2 years
Authors: Xie Tianqu , Liu Yanqun , Chen Xiaoli , He Jing , Yu Yun , Wang Yuchen , Bai Jinbing 
Published: March 2020

Abstract
(1) Background: The colonization characteristics of infant gut microbiota are influenced by many factors at various stages, but few studies have explored the longitudinal effects of environmental tobacco exposure and quantitative weaning time on young children’ intestinal flora. The purpose of this study was to characterize the evolution of intestinal microflora in young children aged 0-2 years and the longitudinal effects of environmental tobacco exposure and weaning time on young children aged 6, 12, and 24 months.(2) Methods: A total of 37 maternal and children pairs were included in a tertiary general hospital in China and followed up for 2 years. General demographic information was collected on mothers (after delivery, 6 months, 12 months) and young children (6 months, 12 months, 24 months), including frequency of exposure and time of weaning, through self-made questionnaires. Fecal samples were collected from mothers in the third trimester of pregnancy and infants at 6, 12 and 24 months, and analyze the microbiota results using the V3-V4 gene sequence of 16S rRNA.(3) Results: the diversity of intestinal microflora in young children was the highest at 24 months and most similar to that in mothers. Weaning time was positively correlated with Lactobacillus in the intestines of infants aged 0 to 12 months. The diversity of microbiota exposed to environmental tobacco smoke at 6 months was lower than that of the non-exposed group, and the higher the exposure at 6-12 months, the lower the abundance of Blautia.(4) Conclusion: The gut microbiota of young children becomes more mature and complicated with age. The extension of complete weaning time to about 12 months is conducive to the colonization of beneficial bacteria. And the long-term exposure of children and the environment of tobacco smoke will affect the dysbiosis of gut microbiota.
The Challenges and Problems Posed by Patient Absconding from Teaching Hospitals in Kerman: A Qualitative Study
Authors: Moradpour Mahnaz , amiresmaili mohammadreza , moghadam Mahmood nekoi , dahesh Tania 
Published: March 2020

Abstract
Background Patient absconding from hospital is one of the permanent crises the hospital face which poses many risks and challenges to the patient, hospital and society. Considering the importance of this subject and numerous problems caused by this phenomenon in the hospitals, the present study aimed to identify the challenges and problems of patient absconding behavior for teaching hospitals of Kerman university of medical sciences.Methods The present study is a qualitative study which was conducted using purposive sampling through semi structured interviews with 63 informants involved in patient treatment process. Data were analyzed using Collaizi content analysis method.Results Two main themes of economic and social factors, and factors related to the hospital covering 36 subthemes were identified to explain the challenges and obstacles of patients absconding behavior. Also, three subthemes of problems for staff, patient and hospital were identified as problems resulted from patient absconding behavior.Conclusion There are many challenges and obstacles to decrease patient absconding from hospitals, and one of the main challenges is the patients’ economic and social problems. The absconding behavior can lead to harm and problems for the patient, and some emotional and occupational consequences for the employees and nurses. Paying attention to this issue and considering some courses of action to prevent patient absconding can lead to a considerable increase in the public trust and eliminate many problems for hospitals.
Laparoscopic Surgery Management of Hydronephrosis Secondary to Left Paraduodenal Hernia in Cryptorchid: Case Report and a Literature Review
Authors: Wang Xin , Guan Yong , Wu Yong , Meng QingYa 
Published: March 2020

Abstract
Background: Left paraduodenal hernia (PDH) is a mesenterico-parietal hernia with retroperitoneal retrocolic herniation of the small bowel into a sac formed by a peritoneal fold located near the fourth portion of the duodenum. It’s a rare cause of hydronephrosis by compressed the upper left ureter. Traditionally, PDH are treated by laparotomy.Case presentation: An 8-year-old boy’s primary purpose was the treatment of cryptorchidism. But hydronephrosis was detected and it was induced by left paraduodenal hernia. Diagnostic laparoscopic exploration confirmed the diagnosis left paraduodenal hernia secondary hydronephrosis. Cryptorchidism was the primary purpose of treatment. The patient was treated with laparoscopic surgery, fixed left paraduodenal hernia, released the ureteropelvic junction obstruction (UPJO) and treated hydronephrosis.Conclusion: Secondary surgery for treating cryptorchidism would be performed and the long-term follow up was necessary.This case suggests us a rare cause of hydronephrosis in children. Every why has a wherefore. We must pay much attention to any unusual details in our medical work even though these details aren’t related to our primary objects.
Overexpression of Long noncoding RNA Oprm1 Attenuates Myocardial Ischemia/Reperfusion Injury by Increasing Endogenous Hydrogen Sulfide Production via Oprm1/miR-30b-5p/CSE axis
Authors: Hu Xiaomin , Liu Bojiang , Wu Peng , Lang Yuheng , Li Tong 
Published: March 2020

Abstract
Background: Ischemia/Reperfusion (I/R) Injury largely limits the efficacy of revascularization in acute myocardial infarction. Long noncoding RNA (lncRNA) Oprm1 is protective in cerebral I/R injury. However, the effect of lncRNA Oprm1 on myocardial I/R injury and its mechanism remains unknown.Methods: We ligated and then released the left anterior descending coronary artery of adult male rats to build the I/R model in vivo, while an H9c2 cardiomyocytes hypoxia-reoxygenation (H/R) model was also used. Myocardial infarction area, cardiac function, histology, Tunnel staining, cell viability, and vital protein expression was conducted and compared.Results: lncRNA Oprm1 was significantly down-regulated in the I/R injury model. When administered with the AAV9-Oprm1 vector, the myocardial injury and cardiac function were mitigated and preserved, with apoptosis reduced. The cystathionine-γ-lyase (CSE) expression and hydrogen sulfide (H2S) expression were increased. The dual-luciferase reporter gene revealed the targeted relationship between lncRNA Oprm1 and miR-30b-5p. In H9c2 cardiomyocytes models, the miR-30b-5p blocked the protective effect of lncRNA Oprm1 on H/R injury, when Bcl-2, Bcl-xl was down-regulated, and HIF-1α, Bnip-3, Caspase-3, and Caspase-9 up-regulated.Conclusions: lncRNA Oprm1can competitively combines with miR-30b-5p, which down-regulates the expression of CSE. When administered with lncRNA Oprm1, increased endogenous H2S can reduce apoptosis and protect the myocardium from I/R injury via activating PI3K/Akt pathway and inhibiting HIF1-α activity.
Screening and identification of significant genes in esophageal squamous cell carcinoma by bioinformatical analysis
Authors: Ren Weirui , Zhang Chuang , Pan Lei , Wang Weijing , Zhao Wenjuan , Zhang Yuxiang , Wang Junmin 
Published: March 2020

Abstract
Background: Esophageal squamous cell carcinoma (ESCC) is one of the most common cancers with notably high incidence and mortality rates. However, the molecular mechanism underlying ESCC pathogenesis and prognosis is very complicated. The main objective of our investigation has been to obtain some knowledge of significant genes with poor outcome and their underlying mechanisms.Methods: Gene expression profiles of GSE26886, GSE23400, GSE20347 and GSE17351 were available from GEO database. The differentially expressed genes (DEGs) were identified, and function enrichment analyses were performed. The protein-protein interaction network (PPI) was constructed and the module analysis was performed using STRING and Cytoscape software.Results: A total of 105 DEGs were identified between normal esophagus and ESCC bioinformatical analysis samples. Functional annotations of the common DEGs indicate that extracellular matrix (ECM) remodeling plays a key role in tumor formation and progression.18 hub genes were identified and disease free survival analysis showed that CDKN3, RAD51AP1, KIF4A may be involved in poor prognosis in ESCC patients.Conclusions: DEGs and hub genes identified in the present study help us understand the molecular mechanisms underlying the carcinogenesis and progression of ESCC, and provide candidate targets for diagnosis and treatment of ESCC.