bluuebunny/crossref_metadata_2025_split huggingface dataset:
Homozygous Hypomorphic HNF1A Alleles Are a Novel Cause of Young-Onset Diabetes and Result in Sulfonylurea-Sensitive Diabetes
Authors: Misra Shivani , Hassanali Neelam , Bennett Amanda J. , Juszczak Agata , Caswell Richard , Colclough Kevin , Valabhji Jonathan , Ellard Sian , Oliver Nicholas S. , Gloyn Anna L. 
Published: January 2020

OBJECTIVE
Heterozygous loss-of-function mutations in HNF1A cause maturity-onset diabetes of the young (MODY). Affected individuals can be treated with low-dose sulfonylureas. Individuals with homozygous HNF1A mutations causing MODY have not been reported.

RESEARCH DESIGN AND METHODS
We phenotyped a kindred with young-onset diabetes and performed molecular genetic testing, a mixed meal tolerance test, a sulfonylurea challenge, and in vitro assays to assess variant protein function.

RESULTS
A homozygous HNF1A variant (p.A251T) was identified in three insulin-treated family members diagnosed with diabetes before 20 years of age. Those with the homozygous variant had low hs-CRP levels (0.2–0.8 mg/L), and those tested demonstrated sensitivity to sulfonylurea given at a low dose, completely transitioning off insulin. In silico modeling predicted a variant of unknown significance; however, in vitro studies supported a modest reduction in transactivation potential (79% of that for the wild type; P &lt; 0.05) in the absence of endogenous HNF1A.

CONCLUSIONS
Homozygous hypomorphic HNF1A variants are a cause of HNF1A-MODY. We thus expand the allelic spectrum of variants in dominant genes causing diabetes.
KOMUNITAS NEMATODA PADA BEBERAPA LOKASI PENANAMAN UBI KAYU DI PROVINSI LAMPUNG
Authors: Prayugo Dwi , Swibawa I G , Solikhin Solikhin , Susilo F X 
Published: January 2020

Penelitian ini bertujuan untuk mempelajari keragaman dan kelimpahan komunitas nematoda yang berasosiasi dengan pertanaman ubi kayu (Manihot esculenta Crantz) di Provinsi Lampung. Pengambilan sampel menggunakan metode survai dilakukan di tiga sentra pertanaman ubi kayu yang terletak di Lampung Selatan, Lampung Tengah, dan Lampung Timur. Proses laboratorium dilakukan di Laboratorium Ilmu Hama Tumbuhan Fakultas Pertanian Universitas Lampung. Penelitian dilaksanakan dari bulan Oktober 2017 sampai dengan Februari 2018. Variabel komunitas nematoda yang diamati meliputi Prominance Value (PV) genus, keragaman nematoda yang diukur dengan jumlah genus dan indeks keragaman Shannon dan indeks karagaman Simpsons, kelimpahan seluruh nematoda dan kelimpahan genus nematoda parasit tumbuhan yang dominan. Data keragaman dan kelimpahan nematoda dianalisis ragam dan nilai tengah diuji dengan uji Beda Nyata Terkecil (BNT) pada taraf nyata 5%. Hasil penelitian menunjukkan pada pertanaman ubi kayu di Lampung ditemukan 29 genus nematoda yang terdiri dari 17 genus nematoda parasit tumbuhan dan 12 genus nematoda hidup bebas; genus nematoda parasit tumbuhan yang dominan yaitu Helicotylenchus, Pratylenchus, Meloidogyne, Apelenchus, dan Xiphinema. pada setiap lokasi penanaman ubi kayu ditemukan 11 - 17 genus, Indeks Shannon 2,1- 2,5, dan Indeks Shimpson’s 0,8 - 0,9. 2). Kelimpahan seluruh nematoda yang ditemukan tiap kabupaten berkisar antara 194,3 - 875,0 individu/300cc tanah, nematoda parasit tumbuhan 169,1-746,6 individu/300cc tahah, nematoda hidup bebas 25,2 - 128,4 individu/300cc tanah. 3). Lokasi penanaman ubi kayu mempengaruhi kelimpahan dan keragaman nematoda.
PENGARUH ARAH GULUDAN DAN PEMBERIAN PUPUK ORGANONITROFOS TERHADAP RESPIRASI DAN BIOMASSA KARBON MIKROORGANISME (C-MIK) TANAH SELAMA FASE VEGETATIFTANAMAN UBI KAYU (Manihot esculenta Crantz)
Authors: Saragih Sahel Renegade , Dermiyati Dermiyati , Niswati Ainin , Banuwa Irwan Sukri 
Published: January 2020

Respirasi dan biomassa karbon mikroorganisme (C-mik) tanah merupakan sifat biologi yang dapat dijadikan sebagai indikator kesuburan tanah. Perlakuan guludan dan pemberian pupuk Organonitrofos yang diberikan ke tanah akan mempengaruhi respirasi dan C-mik tanah. Penelitian ini bertujuan untuk mengetahui pengaruh perlakuan guludan, pemberian pupuk Organonitrofos, dan interaksi keduanya terhadap respirasi tanah dan C-mik tanah. Penelitian ini dilaksanakan selama fase vegetatif tanaman ubi kayu, yaitu Desember 2017 s.d. Mei 2018 di Laboratorium Lapang Terpadu, Fakultas Pertanian, Universitas Lampung,menggunakan Rancangan Acak Kelompok Lengkap (RAKL) yang disusun secara faktorial dengan dua faktor. Faktorpertamameliputiperlakuan guludan, yaitu guludan searah lereng (G1) dan guludan memotong lereng (G2). Faktorkeduameliputi pemberian pupuk Organonitrofos, yaitu Organonitrofos 0 t ha-1 (P0) dan Orgononitrofos 20 t ha-1 (P1). Data yang diperoleh diujihomogenitas ragamnya dengan uji Bartlett dan aditivitasnya dengan uji Tukey.Data dianalisis dengan analisis ragam dan dilanjutkan dengan uji BNT pada taraf 5%.Hubungan antara suhu, C-organik, N-total, dan pH tanah dengan respirasidan C-mik tanah diuji dengan uji korelasi. Hasil penelitian menunjukkan bahwa perlakuan guludan tidak berpengaruh nyata terhadap respirasi tanah pada pengamatan 0, 30, 60, 90, 120, dan 150 HST, namun berpengaruh nyata terhadap C-mik tanah pada pengamatan 120 HST. Pemberian pupuk Organonitrofos berpengaruh nyata terhadap respirasi tanah pada pengamatan 150 HST, tetapi tidak berpengaruh nyata terhadap C-mik tanah. Terdapat interaksi antara perlakuan guludan dan pemberian pupuk Organonitrofos terhadap C-mik tanah pada pengamatan 90HST, tetapi tidak berpengaruh nyata terhadap respirasi tanah.
PENGARUH KOMBINASI PUPUK ORGANONITROFOS DAN PUPUK ANORGANIK TERHADAP SIFAT KIMIA TANAH DAN PRODUKSI TANAMAN JAGUNG (Zea mays L.) DI TANAH ULTISOLNATAR PADA MUSIM TANAM KE DUA
Authors: Putra Ryandi Eka , Dermiyati Dermiyati , Afrianti Nur Afni , Buchari Henrie 
Published: January 2020

Pupuk Organonitrofos merupakan pupuk organik yang dirakit dari bahan-bahan kotoran sapi segar, limbah MSG, sabut kelapa, Trichodermasp., serta mikroba penambat N dan pelarut P sehingga diharapkan dapat mengurangi penggunaan pupuk anorganik. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian kombinasi pupuk Organonitrofos dan pupuk anorganik terhadap pertumbuhan dan produksi tanaman jagung, dan melakukan uji Relative Agronomic Effectiviness (RAE) terhadap produksi jagung. Penelitian dilakukan dengan menggunakan Rancangan Acak Kelompok (RAK) yang terdiri dari 11 perlakuan dan 3 ulangan. Perlakuan adalah kombinasi pupuk Organonitrofos dan pupuk anorganik. Hasil penelitian menunjukkan bahwa perlakuan kombinasi pupuk Organonitrofos dan anorganik pada T6 (100% Organonitrofos + 100% NPK) memberikan pengaruh terbaik terhadap fase generatif yang ditunjukkan padavariabel tinggi tanaman, bobot basah brangkasan bobot tongkol dengan kelobot dan bobot tongkol tanpa kelobot dan merupakan dosis paling efektif secara agronomis karena memiliki nilai RAE 425%. Terdapat korelasi yang nyata antara P-tersedia tanah dengan bobot tongkol berkelobot dan P-tersedia tanah dengan bobot tongkol tanpa kelobot jagung.
PREFERENSI PETANI TERHADAP 15 GALUR PADI GOGO DITABALONG-KALIMANTAN SELATAN
Authors: Perdani Ambar Yuswi , Mulyaningsih Enung Sri , Sulistyowati Yuli , Suherman Suherman , Sanjaya Irwan , Prastyo Muhammad 
Published: January 2020

Upaya menghasilkan suatu varietas unggul baru tanaman padi, sudah selayaknya melibatkan calon pengguna seperti petani. Selera petani atau masyarakat di setiap daerah terhadap suatu varietas dapat berbeda bergantung pada pola konsumsi, budaya dan lingkungan setempat. Penelitian ini bertujuan untuk mengetahui preferensi petani terhadap 15 galur harapan padi gogo toleran aluminium. Pengumpulan data dilakukan di Desa Bintang Ara, Kecamatan Bintang Ara, Kabupaten Tabalong, Kalimantan Selatan. Sebanyak 30 responden petani menilai penampilan galur padi gogo pada fase menjelang panen. Pengamatan dilakukan secara visual terhadap karakter agronomis yang tampak. Sejumlah 15 galur harapan padi gogo ditanam pada petak berukuran 1 m x 5 m dengan jarak tanam 20 cm x 20 cm tanpa rancangan percobaan. Selama proses penanaman terdapat sejumlah kendala antara lain tingkat kesuburan tanah rendah dan serangan hama penyakit tanaman. Namun kendala ini tidak mengganggu produksi dan pengamatan. Hasil penjajakan preferensi petani Bintang Ara terpilih 5 nomor galur harapan padi gogo yaitu: 1, 2, 4, 22, dan 23, Galur-galur tersebut dipilih karena memiliki karakteristik sesuai keinginan calon pengguna. Beberapa karakter utama yang dipilih antara lain: tanaman tinggi, sudut daun bendera lebar, malai panjang, umur tanaman dengan kategori sedang. Sementara karakter unggul lainnya yang disukai ialah jumlah anakan produktif banyak, padi mudah rontok, bulir ramping, warna gabah kuning emas, dan aroma beras wangi.
Patient Waiting Time: Gaps And Determinants Of Patients Waiting Time In Hospitals In Our Communities To Receive Quality Services
Authors: Usman Saheed Opeyemi , Olowoyeye E. , Adegbamigbe O. J. , Olubayo G. P. , Ibijola A. A. , Tijani A. B. , Usman I. N. , Fatunmbi O. J. , Omisakin T. C. , Ipinmoye T. 
Published: January 2020

Background: Patient waiting time has been recognized as an important indicator for determining the quality of healthcare services offered by health facilities. Waiting time is strongly related to patients’ satisfaction with the care received at the hospital in general.
Methods: This is a descriptive cross-sectional study carried out in February 2018 among new patients attending general out-patient department (GOPD) of randomly selected hospitals in South-Western Nigeria. A structured pre-tested questionnaire was used to elicit information from 223 patients who were recruited into the study using a convenience sampling method.
Results: The average total clinic waiting time (TCWT) from entry to seeing a medical doctor was 137.02 ± 53.64 minutes. Only 6 (2.7%) met the Institute of Medicine (IOM) recommendation of having at least 90% of patients seen within 30 minutes of their scheduled appointment in contrast to 193 (86.5%) of respondents desiring to wait not more than 30 minutes for whatever reason before been attended to by the doctor. Although, the level of satisfaction was high (81.2%) in this study, patients who waited longer (>180 min) are more likely to be dissatisfied with services rendered in the OPDs (χ² = 20.104, df = 1, P = 0.001). Factors such as few health personnel (81/120) and having an insurance cover contributed significantly to the waiting time (χ² = 19.54, df = 1, P = 0.001).
Conclusion: In a competitively managed health care environment, patient waiting time play an increasingly important role in a clinic’s ability to attract new business. Efforts therefore, should be made not only to reduce the patient waiting time, but health managers should invest in resources and activities that ensure a productive use of the time patients spend in the process of seeking healthcare services.
Гладиатор – новый сорт укропа
Authors: Ibragimbekov M.G. , Khovrin A.N. , Davletbaeva O.R. 
Published: January 2020

Описаны биологические основы культуры укропа. Представлена агротехника выращивания кустового укропа селекции Агрохолдинга «Поиск» для получения товарной зелени в условиях Московской области: выбор участка, подготовка почвы, удобрения, норма посева, уход, уборка на зелень. Приведена характеристика нового сорта укропа – Гладиатор.The characteristic of the new variety of dill – Gladiator is given. The biological basis of culture is described. The agrotechnical cultivation of the bush dill of the selection of the Poisk Agroholding is briefly presented for obtaining commercial greenery in the conditions of the Moscow region: site selection, soil preparation, fertilizer, seeding rate, care, harvesting of greens.
Оценка перспективных гибридов картофеля для условий Иркутской области
Authors: Bolsheshapova N.I. , Burlov S.P. 
Published: January 2020

В статье представлены исследования продуктивности и качества гибридов картофеля в Иркутском государственном аграрном университете имени А.А. Ежевского. По результатам трехлетнего изучения питомника конкурсного испытания картофеля в лесостепной зоне Иркутской области, по комплексу хозяйственно полезных признаков отобраны гибриды, представляющие интерес для селекции и возделывания в производстве.The article presents the research of productivity and quality of potato hybrids in Irkutsk state agrarian University after A.A. Ezhevsky. According to the results of a threeyear study of the nursery competitive testing of potatoes in the foreststeppe zone of the Irkutsk region, the complex of economically useful features selected hybrids of interest for breeding and cultivation in production.
Овощи борщевой группы в России
Authors: Razin A.F. , Shatilov M.V. , Meshсheryakova R.A. , Surikhina T.N. , Razin O.A. , Telegina G.A. 
Published: January 2020

Приведен анализ рынка овощей борщевой группы в Российской Федерации как повсеместно выращиваемых и традиционно признанных в многонациональной России. Показаны объемы производства, нормативная потребность и самообеспеченность по каждой из культур в разрезе по федеральным округам, их импорт и экспорт, цены потребителей и производителей. Даются предложения по совершенствованию механизмов стабильного обеспечения населения России овощами борщевой группы.The analysis of the market of vegetables of borscht group in the Russian Federation as everywhere grown and traditionally recognized in multinational Russia is resulted. The volumes of production, normative need and selfsufficiency for each crop in the context of Federal districts, their import and export, prices of consumers and producers are shown. Offers on improvement of mechanisms of stable providing the population of Russia with vegetables of borscht group are given.
Новый гетерозисный гибрид томата
Authors: Bekov R.Kh. , Maksimov S.V. 
Published: January 2020

Представлен исходный материал для создания нового гибрида томата F1 Заур с коричневой окраской плодов, с высокими вкусовыми качествами и лежкостью. Гетерозисный гибрид предназначен для выращивания в пленочных теплицах фермерских и приусадебных хозяйств России. Дана его характеристика, в том числе в сравнении со стандартом, гибридом F1 Интуиция.The initial material for breeding of a new hybrid of tomato F1 Zaur with brown colour of fruits, with high taste and keeping quality is presented. The heterotic hybrid is intended for growing in plastic greenhouses of farms and household farms of Russia. Its characteristic is given, including in comparison with the standard, hybrid F1 Intuition.
Как снизить повреждение клубней в машинных технологиях
Authors: Kolchin N.N. , Ponomarev A.G. , Petukhov S.N. 
Published: January 2020

Рассмотрены особенности машинных технологий производства картофеля с анализом возникающих при этом повреждений клубней и с оценкой потерь от их возможных повреждений. Показаны пути снижения повреждений клубней при выращивании картофеля по технологиям с меньшим количеством перегрузок клубней. Отмечены разные способы оценки повреждений клубней.Features of machine technologies of production of potatoes with the analysis of the damages of tubers arising thus and with an assessment of losses from their possible damages are considered. The ways of reducing the damage of tubers when growing potatoes by technologies with fewer overloads of tubers are shown. It is noted the use of different methods for assessing damage of tubers.
Перспективные сорта и гибриды свеклы столовой для длительного хранения
Authors: Borisov V.A. , Filroze N.A. , Sokolova L.M. , Kornev A.V. 
Published: January 2020

В результате пятилетних исследований (2013–2017 годы) выяснено, что наиболее перспективные отечественные сорта для длительного хранения – Маришка, Русская односемянная, Фортуна, Деметра, Бордовая ВНИИО, Бордо 237. Среди голландских гибридов можно выделить F1 Пабло и F1 Водан. Эти сорта и гибриды в течение 6–7 месяцев хранения имели минимальные потери продукции от убыли массы и болезней при сохранении высокого качества продукции.As a result of a five year research (2013–2017) found that the most promising domestic varieties for prolonged storage are: Marishka, Russian odnosemyannaya, Fortuna, Demetra, Bordovaya VNIIO, Bordeaux 237. Among Dutch hybrids can distinguish F1 Pablo and F1 Vodan. These varieties and hybrids as a result 6–7 months of storage have a minimal loss of weight loss products and disease and saved high quality of products.
Микрозеленьпроростковгорохаовощного
Authors: Shilo L.M. , Ushakova O.V. , Ushakov V.A. , Pavlov L.V. , Molchanova A.V. 
Published: January 2020

Установлены требования к микрозелени проростков овощного гороха, предназначенных для потребления в свежем виде, выращенных на субстрате или субстратах, не содержащих элементов питания. Проростки проращивают 14 суток, срезают ростки на высоте 10 15 см, транспортируют всеми видами транспорта при условии обеспечения сохранности продукции и в соответствии с правилами перевозок скоропортящихся грузов.The requirements for microgreens of vegetable pea seedlings intended for fresh consumption are established, grown on the substrate or does not contain elements of the supply substrates. Sprouts of vegetable peas germinate within 14 days. Cut the shoots at a height of 10 15 cm then if seedlings of vegetable pea transport with all models of transport while ensuring product integrity and in accordance with the rules of transportation of perishable produce.
ОценкажаростойкихсортовиисходногоматериалаовощныхкультурнаюгеРоссии
Authors: Geraskina N.V. , Timoshenko I.V. , Kuksa L.A. 
Published: January 2020

В условиях открытого грунта на юге России дана оценка новых перспективных жаростойких сортов лука репчатого, редиса и моркови столовой по признаку жаростойкость, выделены перспективные комбинации лука репчатого: АН158, Денвер, F1Талисман, N1BM1005, Денсити, Ред Ром, Л.40 редиса: РБК, Р05, Л97 моркови столовой: Несравненная, Бирючекутская 415.In the conditions of open ground in the south of Russia, the assessment of new, promising varieties of bulb, radish and carrots in the dining room on the basis of heat resistance was given, promising combinations were identified: bulb АN158, Denver, F1Talisman, N1BM1005, Densiti, Red Rom, L.40 radish: RBK, R05, L97 carrots: Nesravnennaya, Biruychekutskaya 415.
Селекция лука репчатого с устойчивостью к пероноспорозу
Authors: Monakhos G.F. , Monakhos S.G. , Alizhanova R.R. 
Published: January 2020

На Селекционной станции имени Н.Н. Тимофеева гибридизацией с донором устойчивости к пероноспорозу F1 Santero, беккроссом, инбридингом и отбором на инфекционном фоне с помощью молекулярного маркера DMR1 создано 15 линий лука репчатого гомозиготного по гену устойчивости Pd1. Оценка в однолетней культуре позволила выделить линии с массой луковиц 100120 г с высокой сохранностью, которые могут быть использованы в селекции F1 гибридов в качестве отцовских компонентов.Fifteen homozygous for Pd1 downy mildew resistance gene onion lines were developed by hybridization followed backcrossing F1 Santero of downy mildew resistant onion accession of European origin and a collection of onion inbred lines from LC Breeding station after N.N. Timofeev. The resistant to downy mildew, caused by Peronospora destructor (Berk.), plants were selected in segregated populations based on disease resistance test and molecular genotyping using DMR1marker. The lines with a mass of bulbs 100 120 g with high preservation, which can be used in the selection of F1 hybrids as paternal components.
Диагностика болезней моркови столовой с использованием лампы Вуда
Authors: Sokolova L.M. , Sedov E.A. , Maslovskii S.A. , Zamyatina M.E. , Karpova N.A. 
Published: January 2020

Представлен способ модернизации метода ломтиков с использованием источника УФ-излучения (лампы Вуда) при оценке устойчивости селекционного материала моркови к грибным болезням из родов Alternaria и Fusarium. Установлено наличие флуоресценции пораженной ткани до наступления видимых признаков развития мицелия на ломтиках моркови. Возможна оценка устойчивости моркови на 1–3-сутки после заражения ломтиков, что позволяет ускорить селекционную работу, а также метод можно использовать для контроля фитосанитарного состояния и выявления скрытых болезней маточников моркови перед их высадкой.The method of modernization of the method of slices using a source of UV radiation (lamp of Wood) in assessing the resistance of the breeding material of carrots to fungal diseases of the genera Alternaria and Fusarium is presented. The presence of fluorescence of the affected tissue before the onset of visible signs of mycelium development on carrot slices was established. It is possible to evaluate the sustainability of carrots on 1–3 days after infection, slices, allowing to accelerate the breeding work, and the method can be used to control the phytosanitary state and the detection of latent disease of carrots before their planting.
Реакция новых сортов картофеля на загущение посадок
Authors: Shabanov A.E. , Kiselev A.I. 
Published: January 2020

Приведены результаты исследований отзывчивости новых сортов картофеля на загущение посадок по урожайности и качеству продукции. Дана экономическая оценка эффективности уплотнения посадок в разных условиях вегетации растений. Рекомендована оптимальная густота стояния для ряда сортов на дерновоподзолистых супесчаных почвах Центрального района Нечерноземья РФ.The article presents the results of research on responsiveness of new potato varieties in the compacting of the landings on the yield and quality of products. Given the economic estimation of efficiency of seal landings in different conditions of vegetation of plants. The optimal density of standing for a number of varieties on sodpodzolic sandy loam soils of the Central region of the nonChernozem region of the Russian Federation is recommended.
Effect of Zinc concentration on the properties of CdS:Zn nanostructures prepared by Ultrasonic method
Authors: Selvapandiyan M  , Karthikeyan S  , R Muthukumar 
Published: January 2020

This work focuses on investigating the effect of Zn concentration on the properties of CdS:Zn nanoparticles. In the typical synthesis, nanostructured CdS:Zn has been prepared using Cadmium nitrate, zinc nitrate, Na2S, citric acid and ethanol as starting materials. The samples were prepared by exposing the precursor solution to ultrasonic waves for a reaction time of 3 hrs. The precipitate was collected, washed, dried and calcinated at a temperature of 500°C for 1 hour. The samples have been subjected to various characterizations. The phase and structure of the samples have been identified using X-ray diffraction. The spherical morphology of the synthesized particles have been identified by Scanning electron microscope (SEM). The absorbance spectra have been recorded in the UV-Visibleregion and analyzed. The FTIR spectra shows the presence of different functional group present in the synthesized nanoparticles
Strengthening the Bill of Rights
Authors: Butler Andrew S 
Published: January 2020

This article was presented as a Victoria University of Wellington Centennial Lecture during Law Festival week in 1999. The author critically examines two aspects of the current operational structure of the New Zealand Bill of Rights Act 1990—the subordination of the Bill of Rights to all enactments (section 4) and the issue of "positive vets" of proposed legislation by the Attorney-General (section 7). The author identifies them as weaknesses, and makes suggestions as to possible improvements.