bluuebunny/crossref_metadata_2025_split huggingface dataset:
Liposomal drug delivery of Aphanamixis polystachya leaf extracts and its neurobehavioral activity in mice model
Authors: Shariare Mohammad H. , Rahman Mahbubur , Lubna Shamshad R. , Roy Reeti S. , Abedin Joynal , Marzan Akbar L. , Altamimi Mohammad A. , Ahamad Syed Rizwan , Ahmad Ajaz , Alanazi Fars K. , Kazi Mohsin 
Published: April 2020

AbstractNeurodegenerative diseases (Alzheimer’s, Parkinson’s etc.) causes brain cell damage leading to dementia. The major restriction remains in delivering drug to the central nervous system is blood brain barrier (BBB). The aim of this study was to develop a liposomal drug delivery system of Aphanamixis polystachya leaf extract for the treatment of neurodegenerative diseases such as Alzheimer’s and Parkinson’s disease. In this study GC-MS analysis is used to determine major constituents of Aphanamixis polystachya leaf extract. Liposomal batches of Aphanamixis polystachya leaf extract was prepared using design of experiment (DoE) and characterized using Malvern zetasizer, transmission electron microscopy (TEM), and FT-IR. Stability study of blank and leaf extract loaded liposome were performed in gastric media. In-vivo neurobehavioral and anti-inflammatory studies were performed on mice and rat model respectively. GC-MS data showed that major constituents of Aphanamixis polystachya leaf extract are 2-Pentanone, different acids (Octadec-9-enoic acid, 5-Hydroxypipeloic acid etc.), and Beta-Elemene etc. Malvern Zetasizer and TEM data showed that liposome batches of Aphanamixis polystachya leaf extract were in the range of 120 - 180 nm. Interactions between process parameters and material attributes found to have more impact on the average particle size and polydispersity of liposome batches compared to the impact of each parameter in isolation. Stability studies data suggest that blank and leaf extract loaded liposomes were stable at gastric conditions after 4 hours. In-vivo neurobehavioural study data indicated that significant improvement in the memory function, locomotor activity and ambulatory performance of dementia induced mice was observed for the liposomal batches compared to merely A. polystachya leaf extract.
Breaking conversational rules matters to captive gorillas: A playback experiment
Authors: Pougnault Loïc , Levréro Florence , Mulot Baptiste , Lemasson Alban 
Published: April 2020

AbstractAcross human cultures, conversations are regulated by temporal and social rules. The universality of conversational rules suggests possible biological bases and encourages comparisons with the communicative interactions of nonhuman animals. Unexpectedly, few studies have focused on other great apes despite evidence of proto-conversational rules in monkeys, thus preventing researchers from drawing conclusions on potential evolutionary origins of this behaviour. A previous study showed however that western lowland gorillas engage in soft call interactions that seem temporally- and socially-ruled. Indeed, interactions occurred mainly between individuals close in age who followed a preset response delay, thus preventing call overlap. Here, we experimentally investigated the presence of these rules in a captive gorilla group, using a violation-of-expectation paradigm. Head orientation responses suggest that the respect of response delay matters to subjects, but the importance of the interlocutors’ age proximity appeared less clear. The intensity of the response varied with subjects’ age in a context-dependent way, supporting a possible role of learning. Our findings support the growing number of studies highlighting the importance of vocal turn-taking in animals and a possible sociogenesis of this ability. The capacity to “converse” might have been a key step in the co-evolution of communication and complex sociality.
Decline of the boreal willow grouse (Lagopus lagopus) has been accelerated by more frequent snow-free springs
Authors: Melin Markus , Mehtätalo Lauri , Helle Pekka , Ikonen Katja , Packalen Tuula 
Published: April 2020

AbstractClimate change has influenced a range of species across the globe. Yet, to state a noted decline in the abundance of a given species as a consequence of a specific environmental change, for instance, spatially explicit long-term data are a prerequisite. This study assessed the extent to which prolonged snow-free periods in autumn and spring have contributed to the decline of the willow grouse, the only forest grouse changing into a white winter plumage. Time-series data of willow grouse numbers from summer surveys across the study area were integrated with local data on weather (snow cover), mammalian predator abundance and hunting intensity. Modelling was conducted with a hierarchical Bayesian Poisson model, acknowledging year-, area- and location-specific variability. The results show that while willow grouse numbers had decreased continuously across the study landscapes, the decrease was accelerated at the sites where, and during the years when the preceding April was the most snow-free. This indicates a mismatch between the change into a white winter plumage and the presence of snow, turning the bird into an ill-camouflaged prey. The results thus also confirm past hypotheses where local declines of the species have been attributed to prolonged snow-free periods. Across our study area, autumns and springs have become more snow-free, and the trend has been predicted to continue. Thus, in addition to conservation actions, the future of a species such as the willow grouse is also dependent on its ability to adapt to the changed environmental conditions.
Unprovoked Stabilization and Nuclear Accumulation of the Naked Mole-Rat p53 Protein
Authors: Deuker Marian M. , Lewis Kaitlyn N. , Ingaramo Maria , Kimmel Jacob , Buffenstein Rochelle , Settleman Jeff 
Published: April 2020

AbstractThe naked mole-rat is a subterranean rodent, approximately the size of a mouse, renowned for its exceptional longevity (>30 years) and remarkable resistance to cancer. To explore putative mechanisms underlying the cancer resistance of the naked mole-rat, we investigated the regulation and function of the most commonly mutated tumor suppressor, TP53, in the naked mole-rat. We found that the p53 protein in naked mole-rat embryonic fibroblasts (NEFs) exhibits a half-life more than ten times in excess of the protein’s characterized half-life in mouse and human embryonic fibroblasts. We determined that the long half-life of the naked mole-rat p53 protein reflects protein-extrinsic regulation. Relative to mouse and human p53, a larger proportion of naked mole-rat p53 protein is constitutively localized in the nucleus prior to DNA damage. Nevertheless, DNA damage is sufficient to induce activation of canonical p53 target genes in NEFs. Despite the uniquely long half-life and unprecedented basal nuclear localization of p53 in NEFs, naked mole-rat p53 retains its canonical tumor suppressive activity. Together, these findings suggest that the unique stabilization and regulation of the p53 protein may contribute to the naked mole-rat’s remarkable resistance to cancer.
Ni endoscopic classification for Storz Professional Image Enhancement System (SPIES) endoscopy in the detection of upper aerodigestive tract (UADT) tumours
Authors: Abdullah Baharudin , Rasid Nurul Syeha Abdull , Lazim Norhafiza Mat , Volgger Veronika , Betz Christian Stephan , Mohammad Zahiruddin Wan , Hassan Nik Fariza Husna Nik 
Published: April 2020

AbstractThe diagnostic procedure for upper aerodigestive tract (UADT) tumours is by white light endoscopy (WLE) combined with biopsy. However, WLE has difficulty identifying minute epithelial changes which hinders early diagnosis. Storz Professional Image Enhancement System (SPIES) is designed to enhance the visualization of microvasculature on the mucosal surface and detect any epithelial changes. In this study, we aimed to evaluate the use of Ni endoscopic classification with SPIES endoscopy in the detection of UADT tumours. Fifty-nine patients with suspected UADT tumours underwent WLE followed by SPIES endoscopy. All the tumours were biopsied and sent for histopathological examination (HPE). The kappa index (κ) was used to evaluate the agreement between the methods. The level of agreement between SPIES using Ni classification and HPE showed almost perfect agreement as compared to moderate agreement between WLE and HPE. The sensitivity and specificity for WLE and HPE were 77.5% and 84.2% respectively with positive predictive value (PPV) of 91.2% and negative predictive value (NPV) of 64%. The sensitivity and specificity for SPIES endoscopy using Ni classification and HPE were 97.5% and 94.7% respectively with PPV of 97.5% and NPV of 94.7%. SPIES endoscopy using Ni classification is a valid tool for earlier tumour detection.
Targeting the interaction between RNA-binding protein HuR and FOXQ1 suppresses breast cancer invasion and metastasis
Authors: Wu Xiaoqing , Gardashova Gulhumay , Lan Lan , Han Shuang , Zhong Cuncong , Marquez Rebecca T. , Wei Lanjing , Wood Spencer , Roy Sudeshna , Gowthaman Ragul , Karanicolas John , Gao Fei P. , Dixon Dan A. , Welch Danny R. , Li Ling , Ji Min , Aubé Jeffrey , Xu Liang 
Published: April 2020

AbstractPatients diagnosed with metastatic breast cancer have a dismal 5-year survival rate of only 24%. The RNA-binding protein Hu antigen R (HuR) is upregulated in breast cancer, and elevated cytoplasmic HuR correlates with high-grade tumors and poor clinical outcome of breast cancer. HuR promotes tumorigenesis by regulating numerous proto-oncogenes, growth factors, and cytokines that support major tumor hallmarks including invasion and metastasis. Here, we report a HuR inhibitor KH-3, which potently suppresses breast cancer cell growth and invasion. Furthermore, KH-3 inhibits breast cancer experimental lung metastasis, improves mouse survival, and reduces orthotopic tumor growth. Mechanistically, we identify FOXQ1 as a direct target of HuR. KH-3 disrupts HuR–FOXQ1 mRNA interaction, leading to inhibition of breast cancer invasion. Our study suggests that inhibiting HuR is a promising therapeutic strategy for lethal metastatic breast cancer.
Quantum cascade laser based hybrid dual comb spectrometer
Authors: Consolino Luigi , Nafa Malik , De Regis Michele , Cappelli Francesco , Garrasi Katia , Mezzapesa Francesco P. , Li Lianhe , Davies A. Giles , Linfield Edmund H. , Vitiello Miriam S. , Bartalini Saverio , De Natale Paolo 
Published: April 2020

AbstractFour-wave-mixing-based quantum cascade laser frequency combs (QCL-FC) are a powerful photonic tool, driving a recent revolution in major molecular fingerprint regions, i.e. mid- and far-infrared domains. Their compact and frequency-agile design, together with their high optical power and spectral purity, promise to deliver an all-in-one source for the most challenging spectroscopic applications. Here, we demonstrate a metrological-grade hybrid dual comb spectrometer, combining the advantages of a THz QCL-FC with the accuracy and absolute frequency referencing provided by a free-standing, optically-rectified THz frequency comb. A proof-of-principle application to methanol molecular transitions is presented. The multi-heterodyne molecular spectra retrieved provide state-of-the-art results in line-center determination, achieving the same precision as currently available molecular databases. The devised setup provides a solid platform for a new generation of THz spectrometers, paving the way to more refined and sophisticated systems exploiting full phase control of QCL-FCs, or Doppler-free spectroscopic schemes.
Effect of miR-195-5p on cardiomyocyte apoptosis in rats with heart failure by regulating TGF-β1/Smad3 signaling pathway
Authors: Xie Chun , Qi Huaxin , Huan Lei , Yang Yan 
Published: April 2020

AbstractPurpose: The present study set out to investigate the effect of miR-195-5p on cardiomyocyte apoptosis in rats with heart failure (HF) and its mechanism.Methods: HF rat model and hypoxia/reoxygenation (H/R) cardiomyocyte model were established. miR-195-5p expression and transforming growth factor-β1 (TGF-β1)/signal transduction protein (Smad)3 signaling pathway in HF rats and H/R cardiomyocytes were interfered. miR-195-5p expression was tested by Rt-PCR, TGF-β1/Smad3 signaling pathway related proteins were detected by Western Blot, apoptosis of HF rat cardiomyocytes was tested by TUNEL, and apoptosis of cardiomyocytes induced by H/R was checked by flow cytometry.Results: miR-195-5p was lowly expressed in myocardium of HF rats, while TGF-β1 and Smad3 proteins were high-expressed. Up-regulating miR-195-5p expression could obviously inhibit cardiomyocyte apoptosis of HF rats, improve their cardiac function, and inhibit activation of TGF-β1/Smad3 signaling pathway. Up-regulation of miR-195-5p expression or inhibition of TGF-β1/Smad3 signaling pathway could obviously inhibit H/R-induced cardiomyocyte apoptosis. Dual-luciferase reporter enzyme verified the targeted relationship between miR-195-5p and Smad3.Conclusion: miR-195-5p can inhibit cardiomyocyte apoptosis and improve cardiac function in HF rats by regulating TGF-β1/Smad3 signaling pathway, which may be a potential target for HF therapy.
“Undating” and “Quarter Ponies”: Comparing the Use of Derived Words and Compounds Across Discourse Types and Age Groups
Authors: Squires Bonita , Bird Elizabeth Kay-Raining , Cahill Peter 
Published: April 2020

Purpose
Derivational morphology and compounds are important aspects of academic vocabulary. However, investigation of the development of expressive derivational and compound morphology using language sampling is sparse. This cross-sectional study used three types of language samples to investigate quantitative and qualitative changes in the spontaneous production of derived words and compounds in early and late elementary–age children as a function of age and discourse type.

Method

Twenty-three children in two age groups (early elementary,
n
= 12; late elementary,
n
= 11) participated. Three types of language samples were elicited: conversational (10-min conversation with an adult examiner), narrative (“I tell–you tell” narrative with single picture stimulus combined with a story stem narrative), and expository (explanation of how to play a favorite game or sport with text-based topic prompts). Language samples were transcribed using Systematic Analysis of Language Transcripts (Miller & Chapman, 2012) conventions with the addition of researcher-created codes to identify derived words and compounds. Quantitative measures (number of derived words, different derived words, number of compounds, and different compounds) were calculated as percentages of total words or number of different words to control for differing sample length. The types of derivational morphemes and compounds produced by children in each age group were listed and qualitatively analyzed for evidence of a sequential development of specific morpheme types, variation in complexity, and productivity.

Results
Developmental change in quantitative and qualitative measures of derivational and compound morphology was evident across early and late elementary–age children in the language samples. Lists of derived words and compounds produced provided a rich source for analyzing developmental patterns in expressive morphology. Conversational and, to a lesser extent, expository discourse generated the greatest number and diversity of multimorphemic words.

Conclusions
This research provided new insights into academic vocabulary development in elementary school–age children. The clinical usefulness of language sampling to quantitatively and qualitatively assess derivational morphology and compounds was demonstrated.

Supplemental Material

https://doi.org/10.23641/asha.12170373
LoRa mesh architecture for automation of rural electricity distribution networks
Authors: Prade L.R. , Uberti V.A. , Abaide A.R. , Pereira P.R.S. , de Figueiredo R.M. , Both C.B. 
Published: April 2020

The current transformation scenario in power distribution networks forecasts the need for alternative solutions in communication systems employed in the process of making them intelligent. As a premise, these alternatives should be easy to implement and affordable to allow their adoption in rural distribution networks, where, often, the use of technologies, such as mobile, is not available. This Letter proposes a new communication architecture with mesh topology, based on LoRa technology. The architecture adapts packet prioritisation and MIMO mechanisms to ensure the communication requirements necessary for the services. In this sense, several applications and protocols of a smart grid can coexist in the same communication system. The deployment of the architecture gave rise to a routing protocol adapted to the limitations of the radio technology used. The benefits of the proposed architecture can be observed when compared to the use of traditional low power wide area network networks and cellular technologies. The robustness of the system has been proven with average latency measurement within the acceptable range for DNP3 Modbus and NBR14522 applications. The results have shown its potential employment in the management of intelligent rural distribution systems.
A PubMed Citations Search Study on the Prevalence of Epiphora in the General Adult Population
Authors: Haefliger Ivan O. , Haefliger Yasmina D. , Pimentel de Figueiredo Ana Rosa 
Published: April 2020

Abstract
Purpose To assess the prevalence of epiphora in the general adult population based on PubMed search citations.
Methods 1) Electronic PubMed MEDLINE database search (September 13, 2019) with the terms (Medical Subject Headings or MeSH) “prevalence” and “epiphora”, 2) “epiphora” and “dry eye”, and 3) “prevalence”, “epiphora”, and “dry eye”. Review of all citations from these searches containing the term “epiphora” either in their abstract or title.
Results 1) PubMed search retrieved 2 617 137 citations for “prevalence”, 26 135 for “epiphora”, and 2554 for “prevalence” AND “epiphora”. Within the latter 2554 citations, the word “epiphora” appeared in the abstract or title of only 109 citations (< 5%). None of these 109 citations assessed the prevalence of epiphora in the adult general population as the primary end point. Only one abstract mentioned that out of 125 patients, 7.2% indicated, retrospectively, that they had already had epiphora before cataract surgery. Two large population-based studies addressed the incidence of epiphora, not in adults, but in infants (20%) and children (7.7%). 2) The PubMed search showed 22 487 citations for “dry eye”, 30 211 for “epiphora” OR “dry eye”, and up to 18 414 joint citations for the terms “epiphora” AND “dry eye”. These 18 414 citations were 70 and 82% of the number of MeSH citations for “epiphora” and “dry eye” alone, respectively. Of these 18 414 citations, the word “epiphora” only appeared in 131 citations (< 1%), one of them being an extra report mentioning a 32% incidence of epiphora among postmenopausal women. 3) The search found 2206 citations for “prevalence” AND “epiphora” AND “dry eye”, with only 10 of them (< 1%) containing the word “epiphora”.
Conclusions Despite a large number of citations retrieved by PubMed searches, there seems to be a lack of studies on the prevalence of epiphora in the adult general population. There is apparently also a large number of overlapping PubMed citations retrieved for searches with the terms “epiphora” AND “dry eye”, although more than 99% of them did not even display the word “epiphora”. Although epiphora is considered a common complain, its prevalence in the adult general population deserves to be further assessed.
GLYCEMIC AND LIPIDEIC STATUS IN PATIENTS WITH ACUTE MYOCARDIAL INFARCTION
Authors: Rao A. Harish 
Published: April 2020

Abstract:
Objective: to know the glycemic and lipidaemic status in patients with acute myocardial infarction, and with the secondary objective to know the effect of age, gender, diabetes, smoking, hypertension on fasting glucose and lipid levels.
Methods and materials: The 74 patients admitted for acute myocardial infarction during the study period of one year were analysed for fasting glucose values and serum levels of total cholesterol, HDL cholesterol, LDL cholesterol and triglycerides.
Results: The mean serum concentrations of total cholesterol, triglycerides, HDL cholesterol and LDL cholesterol were 233.28±45.34, 139.22±41.71, 171.43±36.53 and 27.07±36.53 respectively. Mean levels of total cholesterol, HDL cholesterol, triglycerides and fasting glucose values were not affected by age, gender, BMI, hypertension and smoking. BMI >30kg/m2 was associated with increased levels of total cholesterol(p=0.013) and LDL cholesterol(p=0.014). Also increase LDL cholesterol was seen in male gender(p=0.04). The prevalence of hypercholesterolemia, hypertriglyceridemia and low HDL cholesterol was 82.4%,77% and 78% respectively. Diabetes had no effect on lipid profile.
Conclusion: our study highlighted the prevalence of dyslipidemias associated with myocardial infarction but not significant impact of fasting glucose levels.
EFFECTIVENESS OF DEEP BREATHING EXERCISE (DBE) ON THE HEART RATE VARIABILITY, BP, ANXIETY & DEPRESSION OF PATIENTS WITH CORONARY ARTERY DISEASE
Authors: D'silva Fatima , H. Vinay , Muninarayanappa N.V. 
Published: April 2020

Abstract:Psychosocial risk factors significantly contribute to the morbidity and mortality of patients with cardiovascular disorders. The present study explored the anxiety and depression status of patients with coronary artery disease and evaluated the effect of deep breathing exercise on these psychosocial variables as well as physiological variables like heart rate variability and blood pressure. A randomized control design was adopted for the study. Out of 65 clients eligible for the study, 45 were selected based on inclusion criteria. Patient were trained in Deep breathing exercise (DBE)for 2-3 days, were instructed to practice the exercise twice a day for 10 min for a period of 2 weeks, further instructed to come for follow up to cardiac OPD after 2 weeks. The study findings revealed that majority of the cardiac patients were anxious 39 (86.66%), 23(57.5%) had mild depression and 3(7.5%) were with severe depression. Fischer's exact test revealed a significant association between depression and occupation (p=0.051), monthly income (p=0.031) and co morbid disease (p=0.006, p<0.05). Karl Pearson's correlation coefficient revealed significant positive correlation between anxiety and depression i.e. (r = 0.414, p <0.01). DBE was found to be effective in reducing anxiety and diastolic BP of clients with CAD. But there was no significant reduction in HR, SBP and depression after the intervention.