bluuebunny/crossref_metadata_2025_split huggingface dataset:
Influenza outbreak among students in Ghana: a report from three time points
Authors: Sylverken Augustina Angelina , Owusu Michael , Yeboah Richmond , El-Duah Philip , Gorman Richmond , Bonney Joseph Kofi , Aryeetey Sherihane , Adu Eric , Amoako Yaw Ampem , Adade Titus , Yeboah Eric , Bonney Joseph , Ayisi-Boateng Nana Kwame , Drosten Christian , Phillips Richard Odame 
Published: September 2020

Abstract
Background: Influenza viruses are known to be responsible for a number of respiratory disease outbreaks worldwide. While there exists documented information on influenza which allows for effective strategic preparedness activities in industrialized countries, the same cannot be said of developing and underdeveloped countries. It therefore behoves on developing countries to document information on circulating strains of influenza. Such information will enable prioritization of prevention and more targeted preparedness strategies. In this paper, we report on three different outbreaks of Influenza A among Ghanaian students in December 2017, May 2019 and December 2019 in the Ashanti region of Ghana.Methods: Throat or nasal samples were collected from all students who presented with signs and symptoms of flu with flocked swabs, stored in RNAlater and transported to the laboratories of Kumasi Centre for Collaborative Research (KCCR) at ambient temperature. Following viral RNA extraction, detection of viral nucleic acid of both influenza A and its subtypes including H1N1 and H3N2 was done by real-time reverse transcription Polymerase Chain Reaction (RT-PCR).Results: A total of 112 samples were taken. An overall Influenza A prevalence of 51.8% (95% CI = 42.1-61.3) was recorded during the three outbreak time points. Of these, 11 (19.0%) were confirmed to be H1N1 (pdm09) and 47 (81.0%) were H3N2 strains. All the 11 H1N1 (pdm09) were identified during the first outbreak. The H3N2 strains were identified during the second and third outbreaks in May 2019 and December 2019 respectively. Our results show that influenza A virus activity was higher during the months of November and December compared to May. Age, contact with infected subjects, temperature, fever within the last 24 hours and headaches showed significant variations (p-value <0.01) with respect to influenza A. Wald’s test revealed that subjects presenting with headaches had about 5 times odds (95% CI = 1.4-17.7) for influenza A infection compared to subjects without headache (p = 0.014).Conclusion: Our study provides epidemiological information to better understand the incidence and burden of influenza to allow for prioritisation of prevention and control strategies. Such information would aid in controlling and preparing for future influenza epidemics.
In vitro propagation of three mosaic disease resistant cassava cultivars
Authors: SESSOU Amitchihoué Franck , Kahia Jane W. , Houngue Jerome Anani , Ateka Elijah Miinda , Dadjo Colombe , Ahanhanzo Corneille 
Published: September 2020

Abstract
BackgroundCassava is a staple food for over 800 million people globally providing a cheap source of carbohydrate. However, the cultivation of cassava in the country is facing to viral diseases, particularly cassava mosaic disease (CMD) which can cause up to 95% yield losses. With aim to supply farmers demand for clean planting materials, there is need to accelerate the production of the elite cultivars by use of tissue culture in order to cope with the demand.MethodsNodal explants harvested from the greenhouse grown plants were sterilised using different concentrations of a commercial bleach JIK (3.85% NaOCl) and varying time intervals. Microshoots induction was evaluated using thidiazuron (TDZ), benzyl amino purine (BAP), and kinetin. Rooting was evaluated using different auxins (Naphthalene acetic acid NAA and Indole-3-butyricacid IBA). PCR-based SSR and SCAR markers were used to verify the presence of CMD2 gene in the regenerated plantlets.ResultsThe highest level of sterility in explants (90%) was obtained when 20% Jik was used for 15 minutes. The best cytokinin for microshoots regeneration was found to be kinetin with optimum concentrations of 5, 10 and 20 µM for Agric-rouge, Atinwewe, and Agblehoundo respectively. Medium without growth regulators was the best for rooting the three cultivars. A survival rate of 100%, 98%, and 98% was recorded in the greenhouse for Agric-rouge, Atinwewe, and Agblehoundo respectively and the plantlets appeared to be morphologically normal. The SSR and SCAR analysis of micropropagated plants showed a profile similar to that of the mother plants indicating that the regenerated plantlets retained the CMD2 gene after passing through in vitro culture, as expected with micropropagation.Conclusion The nodal explants was established to be 20% of Jik (3.85% NaOCl) with an exposure time of 15 minutes. Kinetin was proved to be the best cytokinins for microshoot formation with the optimum concentration of 5, 10 and 20µM for Agric-rouge, Atinwewe, and Agblehoundo respectively. The protocol developed during this study will be useful for mass propagation of the elite cassava cultivars.
Comparison of frontal alpha asymmetry among schizophrenia patients, major depressive disorder, and healthy controls
Authors: Jang Kuk-In , Lee Chany , Lee Sangmin , Huh Seung , Chae Jeong-Ho 
Published: September 2020

Abstract
Background: Electroencephalography (EEG) frontal alpha asymmetry (FAA) has been observed in several psychiatric disorders. Dominance in left or right frontal alpha activity remains inconsistent in patients with major depressive disorder (MDD), patients with schizophrenia, and healthy controls. This study compared FAA among patients with MDD and schizophrenia, and healthy controls.Methods: We recruited 20 patients with MDD, 18 patients with schizophrenia, and 16 healthy individuals. The EEG alpha frequency ranged from 8 Hz to 12 Hz. FAA was expressed as the difference between absolute power values of right and left hemisphere electrodes in the alpha frequency range (common-log-transformed frontal right- and left-hemisphere electrodes: F4–F3, F8–F7, FP2–FP1, AF4–AF3, F6–F5, and F2–F1). Hamilton depression and anxiety rating scales were evaluated in patients with MDD. Positive and negative syndrome scales were evaluated in patients with schizophrenia.Results: Patients with schizophrenia showed significantly lower left FAA than healthy controls (F4–F3, schizophrenia vs. healthy controls: -0.10 ± 0.04 vs. -0.05 ± 0.05). There were no significant differences in FAA between patients with schizophrenia and MDD as well as between patients with MDD and healthy controls.Conclusions: The present study suggests that FAA indicates a relatively lower activation of left frontal electrodes in schizophrenia. The left-lateralized FAA could be a neuropathological attribute in patients with schizophrenia, but a lack of sample size and information such as medication and duration of illness might obscure the interpretation and generalization of our findings. Thus, further studies to verify the findings would be warranted.
COVID patients encountered by a general surgeon- 6 unique scenarios.
Authors: Jain Sumita , Mehta Deeksha , Aggarwal Lakshman , Goyal Alka , Sharma Chandra Shekhar , Mangalhara Naresh Kumar , Dadhich Yogendra , Bansal Somendra 
Published: September 2020

Abstract
The authors have requested that this preprint be withdrawn due to author disagreement.
Continuous glucose monitoring in older people with diabetes receiving home care - A feasibility study.&nbsp;
Authors: Larsen Annette Bævre , Hermann Monica , Graue Marit 
Published: September 2020

Abstract
Background Hypoglycemic incidents in older people can cause severe health problems, enhance general age-related disabilities and cause frailty. Little is known about incidences of hypoglycemia in older home-dwelling people with diabetes. Thus, the aim of this study was to examine the feasibility of capturing hypoglycemia and issues associated with increased risk of hypoglycemia by use of continuous glucose monitoring (CGM) and standardized questionnaires among older home dwelling individuals with diabetes type 2 receiving home care. MethodsCGM with the Ipro2 blinded monitoring system were performed for five days in six home-dwelling individuals ≥ 75 years diagnosed with diabetes and receiving home care. Demographic (age, gender, living arrangements) and clinical data (diabetes diagnoses and duration, diabetes medication, documented treatment goal, available glycosylated hemoglobin (HbA1c),) were collected from electronic patient records in home care services. Feasibility (ease of use, quality of data, time spent) of standardized questionnaires to identify the risk of hypoglycemia (the McKellar Risk Assessment Tool), risk of malnutrition (the Mini Nutritional Assessment (MNA)), functional status (the Individual-based Statistics for Nursing and Care Services (IPLOS)) and cognitive status (the Mini Mental Status Exam (MMSE)) was also assessed. Questionnaire data was collected by a study nurse in the individuals’ home. Results The practical use of CGM was satisfactory, with no major remarks about discomfort or technical errors, except for one participant with skin reaction (redness). Collecting data with the McKellar Risk Assessment Tool, MNA and IPLOS worked well according to quality of data, time spent and ease of use. The MMSE survey required extensive training of personnel to be conducted. ConclusionThe feasibility study informs an upcoming study on the incidence and risk factors of hypoglycemia in home dwelling older individuals, where we will reconsider the use of the MMSE questionnaire. The use of blinded CGM in this population was well tolerated and can be used ‘as is’ for future studies.
Effect of dapsone alone and in combination with intracellular antibiotics against the biofilm form of B. burgdorferi
Authors: Horowitz Richard Ira , Murali Krithika , Gaur Gauri , Freeman Phyllis R. , Sapi Eva 
Published: September 2020

Abstract
Objective: Lyme disease is a tick-borne, multisystemic disease caused by Borrelia burgdorferi. Standard treatments for early Lyme disease include short courses of oral antibiotics but relapses often occur after discontinuation of treatment. Several studies have suggested that ongoing symptoms may be due to a highly antibiotic resistant form of B. burgdorferi called biofilms. Our recent clinical study reported the successful use of an intracellular mycobacterium persister drug used in treating leprosy, diaminodiphenyl sulfone (dapsone), in combination therapy for the treatment of Lyme disease. In this in vitro study, we evaluated the effectiveness of dapsone individually and in combination with cefuroxime and/or other antibiotics with intracellular activity including doxycycline, rifampin, and azithromycin against Borrelia biofilm forms utilizing crystal violet biofilm mass, and dimethyl methylene blue glycosaminoglycan assays combined with Live/Dead fluorescent microscopy analyses. Results: Dapsone, alone or in various combinations with doxycycline, rifampin and azithromycin produced a significant reduction in the mass and protective glycosaminoglycan layer and overall viability of B. burgdorferi biofilm forms. This in vitro study strongly suggests that dapsone combination therapy could represent a novel and effective treatment option against the biofilm form of B. burgdorferi.
Nigella A ameliorates inflammation and intestinal flora imbalance in DSS induced colitis mice
Authors: Hu XingJiang , Xu Nana , Yang Xi , Hu Xi , Zheng Yunliang , Zhang Qiao 
Published: September 2020

Abstract
Nigella A, also named Sieboldianoside A, has been extracted from many kinds of Traditional Chinese Medicine (TCM), such as Nigella glandulifera, Stauntonia chinensis DC., and the leaves of Acanthopanax sieboldianus. Nigella A exhibited potential analgesic, anti-inflammatory, anti-tumor, and antioxidant activities. However, whether Nigella A could treat ulcerative colitis (UC) is still unknown. As saponins always be regarded as the kinds of ingredients that could regulate immunity and intestinal flora. This research aimed to investigate the therapeutic effect of Nigella A on UC and explore its effect on intestinal flora. We noted that Nigella A and Sulfasalazine (SASP) could significantly improve the signs and symptoms, alleviate colonic pathological injury in DSS-induced mice. The changing of many specific bacterial genus such as Lactobacillus, Porphyromonadaceae, Bacteroides and Escherichia might closely related to the recovery of intestinal inflammatory response. This study initially confirmed the therapeutic effect of Nigella A and SASP on DSS-induced colitis by improving the diversity of intestinal microbial composition. Nigella A has the potential to be developed for the treatment of UC and other disorders related to the imbalance of intestinal flora.
Identifying the key nodes of HIV molecular transmission network among men who have sex with men in Guangzhou, China: A cross-sectional study
Authors: Yang Juan , Han Zhigang , Xu Huifang , Xing Hui , Cheng Weibin , Gu Yuzhou , Xu Peng , Lv Fan 
Published: September 2020

Abstract
Background

Identifying the most influential spreaders in HIV transmission networks is crucial to develop effective prevention strategies. The purpose of this study is to identify the key nodes of the molecular transmission network among MSM, which has significant insight into developing effective HIV prevention strategies.
Methods

We analyzed HIV-1 pol sequences provided through the Center for Disease Control and Prevention, Guangzhou, China. Sequences were obtained from newly-HIV diagnosed MSM during 2015–2017. We calculated pairwise genetic distance, identified linked pairs of sequences (those with distance ≤ 1.5%), and examined key nodes of these potential transmission partners.
Results

Of 184 HIV recently diagnosed men who have sex with men (MSM), 40.76% were linked to other MSM. Social network analysis demonstrated that there were 9 key nodes were detected. From the results of multivariate logistic regression model, young MSM born in the 1990s and 1980s was 0.06 and 0.12 times as likely to be a key node as was the older MSM born in the 1970s and before.
Conclusions

There were a lot of subgroups connected by sharing comembers in HIV molecular transmission network among MSM in Guangzhou. Some HIV-infected MSM called as key nodes mediated the transmission of HIV among different subpopulations. Young MSM were less likely to promote HIV transmission than older MSM.
A Sharp-Interface Immersed Boundary Method for Simulating High-Speed Compressible Inviscid Flows
Authors: Wang Junjie , Gu Xiangyu , Wu Jie 
Published: September 2020

Abstract
This paper presents a robust sharp-interface immersed boundary method for simulating inviscid compressible flows over stationary and moving bodies. The flow field is governed by Euler equations, which are solved by using the open source library OpenFOAM. Discontinuities such as those introduced by shock waves are captured by using Kurganov and Tadmor divergence scheme. Wall-slip boundary conditions are enforced at the boundary of body through reconstructing flow variables at some ghost points. Their values are obtained indirectly by interpolating from their mirror points. A bilinear interpolation is employed to determine the variables at the mirror points from boundary conditions and flow conditions around the boundary. To validate the efficiency and accuracy of this method for simulation of high-speed inviscid compressible flows, four cases have been simulated as follows: supersonic flow over a 15 angle wedge, transonic flow past a stationary airfoil, a piston moving with supersonic velocity in a shock tube and a rigid circular cylinder lift-off from a flat surface triggered by a shock wave. Compared to the exact analytical solutions or the results in literature, good agreement can be achieved.
Breastfeeding practice in China from 2013 to 2018: a study from a national dynamic follow-up surveillance
Authors: Kang Leni , Liang Juan , He Chunhua , Miao Lei , Li Xiaohong , Dai Li , Li Qi , Liu Zheng , Zhu Jun , Wang Yanping , Liu Hanmin 
Published: September 2020

Abstract
Background

Breastfeeding is important for the physical and psychological health of the mother and child. Basic data on breastfeeding practices in China are crucial for understanding how to advance health-related goals.

Methods

This was an observational study. We used data from the Under-5 Child Nutrition and Health Surveillance System in China for the period 2013-2018. The prevalence of early initiation of breastfeeding (EIBF) and exclusive breastfeeding (EBF) were calculated for each year for subgroups of China. The Cochran-Armitage test was used to explore the time trends. The annual percent of change (APC) were calculated by log-linear regression followed by exp transformation.

Results

The prevalence of EIBF and EBF increased significantly from 44.57% (95% CI: 44.07, 45.07) and 16.14% (95% CI: 15.10, 17.18) in 2013 to 55.84% (95% CI: 55.29, 56.38) and 34.90% (95% CI: 33.54, 36.26) in 2018 (P trend <0.001). The respective APCs were 4.67% (95% CI: 3.51, 5.85) and 14.90% (95% CI: 9.97, 20.04). Increases were observed in both urban and rural areas, with urban areas showing greater APCs for EIBF (6.05%; 95% CI: 4.22, 7.92 v.s. 2.26%; 95% CI: 1.40, 3.12) and EBF (18.21%; 95% CI: 11.53, 25.29 v.s. 9.43%; 95% CI: 5.52, 13.49). The highest EBF prevalence was observed in the East, but the Central area showed the highest APC. The prevalence of EBF decreased with increasing age during the first 6 months, especially after 3 months.

Conclusion

The prevalence of both EIBF and EBF in China are improving in recent years. The rural and West China could be the key areas in the future actions. More efforts should be made to protect and promote breastfeeding to achieve near- and long-term goals for child health.
Investigating the genetic architecture of disease resilience in pigs by genome-wide association studies of complete blood count traits collected from a natural disease challenge model
Authors: Bai Xuechun , Yang Tianfu , Putz Austin , Wang Zhiquan , Li Changxi , Fortin Frédéric , Harding John , Dyck Michael , Canada PigGen , Dekkers Jack , Field Catherine , Plastow Graham 
Published: September 2020

Abstract
BackgroundGenetic improvement for disease resilience is anticipated to be a practical method to improve efficiency and profitability of the pig industry, as resilient pigs maintain a relatively undepressed level of performance in the face of infection. However, multiple biological functions are known to be involved in disease resilience and this complexity means that the genetic architecture of disease resilience remains largely unknown. Here, we conducted genome-wide association studies (GWAS) of 465,910 autosomal SNPs for complete blood count (CBC) traits that are important in an animal’s disease response. The aim was to identify the genetic control of disease resilience.ResultsUnivariate and multivariate single-step GWAS were performed on fifteen CBC traits measured from the blood samples of 2743 crossbred (Landrace × Yorkshire) barrows drawn at 2-weeks before, and at 2 and 6-weeks after exposure to a polymicrobial infectious challenge. Overall, at a genome-wise false discovery rate of 0.05, five genomic regions located on Sus scrofa chromosome (SSC) 2, SSC4, SSC9, SSC10, and SSC12, were significantly associated with white blood cell traits in response to the polymicrobial challenge, and nine genomic regions on multiple chromosomes (SSC1, SSC4, SSC5, SSC6, SSC8, SSC9, SSC11, SSC12, SSC17) were significantly associated with red blood cell and platelet traits collected before and after exposure to the challenge. By functional enrichment analyses using Ingenuity Pathway Analysis (IPA) and literature review of previous CBC studies, candidate genes located nearby significant single-nucleotide polymorphisms were found to be involved in immune response, hematopoiesis, red blood cell morphology, and platelet aggregation.ConclusionsThis study helps to improve our understanding of the genetic basis of CBC traits collected before and after exposure to a polymicrobial infectious challenge and provides a step forward to improve disease resilience.
Postnatal&nbsp;Service Utilization and Associated Factors among Women who gave Birth in the last 12 months Prior to Study Period&nbsp;in Jimma Town, Southwest&nbsp;Ethiopia.
Authors: Abera Beyene , Araya Fitsum , Rad Mulugeta , Ysuf Elias Ali 
Published: September 2020

Abstract
BACKGROUND: - Postnatal care is care (PNC) given to mother and new born within first 42 days of delivery. Lack of care in this period may result in disability even death of either of mother or newborn or both. The aim of this study was to determine postnatal service utilization and associated factors among women, who gave birth in the last 12 months prior to May, 2019.METHODS: Community based cross-sectional study was conducted on 420 women who had given birth prior to May 1 to June 30, 2019(study period). The 17 kebeles of Jimma town is grouped into three similar groups based on occupation of majority of population. Three kebeles were randomly selected from each group. Data was collected using a pretested structured questionnaire. Descriptive statistic, such as frequencies, proportions and means were calculated. In bivariate logistic regression variables with p-value less than 0.25 were selected for multiple logistic analyses and multiple logistic regression was conducted. Finally significantly associated variables were declared at p value less than 0.05.Result:-This study showed that prevalence of postnatal care utilization was 59.5%. Mothers who knew at least one danger signs which comes after delivery (AOR=11.5,(95%CI:5.56,22.7),who attended ANC (AOR=5.4, 95%CI:2.7,11.3 ), who get counseled to attend PNC ( AOR=14.9% CI :6.1,31.4),who attended at least primary school (AOR=2.3 ,95% CI:1.75,7.0),who did not travel more than 30 minutes to reach health facility(AOR=6.8,95% CI 3.4, 13.6 and those having more family size were more likely utilized postnatal service than their counterpartConclusions: - In this study utilization of postnatal care service was very far behind national target (which is 95% at the end of 2020, health sector transformational plan). Awareness about danger signs, educational status of the mother, counseling to attend to attend postnatal care, distance from health facility and family size were strongly associated with PNC utilization.