bluuebunny/crossref_metadata_2025_split huggingface dataset:
One Health, One Hive: A scoping review of honey bees, climate change, pollutants, and antimicrobial resistance
Authors: de Jongh Etienne J. , Harper Sherilee L. , Yamamoto Shelby S. , Wright Carlee J. , Wilkinson Craig W. , Ghosh Soumyaditya , Otto Simon J.G. 
Published: November 2020

AbstractAnthropogenic climate change and increasing antimicrobial resistance (AMR) together threaten the last 50 years of public health gains. Honey bees are a model One Health organism to investigate interactions between climate change and AMR. The objective of this scoping review was to examine the range, extent, and nature of published literature on the relationship between AMR and honey bees in the context of climate change and environmental pollutants.The review followed systematic search methods and reporting guidelines. A protocol was developeda prioriin consultation with a research librarian. Resulting Boolean search strings were used to search Embase® via Ovid®, MEDLINE®, Scopus®, AGRICOLA™ and Web of Science™ databases. Two independent reviewers conducted two-stage screening on retrieved articles. To be included, the article had to examine honey bees, AMR, and either climate change or environmental pollution. Data, in accordance with Joanna Briggs Institute guidelines, were extracted from relevant articles and descriptively synthesized in tables, figures, and narrative form.A total of 21 articles met the inclusion criteria, with almost half of all articles being published in the last five years (n=10). These articles predominantly investigated hive immunocompetence and multi-drug resistance transporter downregulation (n=10), susceptibility to pests (n=15), especially American foul brood (n=9), and hive product augmentation (n=3).This review identified key themes and gaps in the literature, including the need for future interdisciplinary research to explore the link between AMR and environmental change evidence streams in honey bees. We identified three potential linkages between pollutive and climatic factors and risk of AMR. These interconnections reaffirm the necessity of a One Health framework to tackle global threats and investigate complex issues that extend beyond honey bee research into the public health sector. It is integral that we view these “wicked” problems through an interdisciplinary lens to explore long-term strategies for change.
The spatial self-organization within pluripotent stem cell colonies is continued in detaching aggregates
Authors: Elsafi Mabrouk Mohamed H. , Goetzke Roman , Abagnale Giulio , Yesilyurt Burcu , Salz Lucia , Zeevaert Kira , Ma Zhiyao , Toledo Marcelo A. S. , Li Ronghui , Costa Ivan G. , Pachauri Vivek , Schnakenberg Uwe , Zenke Martin , Wagner Wolfgang 
Published: November 2020

SummaryColonies of induced pluripotent stem cells (iPSCs) reveal aspects of self-organization even under culture conditions that maintain pluripotency. To investigate the dynamics of this process under spatial confinement, we used either polydimethylsiloxane (PDMS) pillars or micro-contact printing of vitronectin. There was a progressive upregulation of OCT4, E-cadherin, and NANOG within 70 µm from the outer rim of iPSC colonies. Single- cell RNA-sequencing demonstrated thatOCT4highsubsets have pronounced up-regulation of the TGF-β pathway, particularly of NODAL and its inhibitor LEFTY, at the rim of the colonies. Furthermore, calcium-dependent cell-cell interactions were found to be relevant for the self-organization. Interestingly, after 5 to 7 days, the iPSC colonies detached spontaneously from micro-contact printed substrates to form 3D aggregates. This new method allowed generation of embryoid bodies (EBs) of controlled size, without any enzymatic or mechanical treatment. Within the early 3D aggregates, the radial organization and differential gene expression continued in analogy to the changes observed during self-organization of iPSC colonies. Our results provide further insight into the gradual self-organization within iPSC colonies and at their transition into EBs.
Forkhead box R1-mediated stress response linked to a case of human microcephaly and brain atrophy
Authors: Mota Andressa , Hong Rui , Niu Sheng-Yong , Bertherat Féodora L. , Wolfe Lynne , Malicdan Christine May , Markello Thomas C. , Adams David R. , Gahl William A. , Cheng Christine , Beffert Uwe , Ho Angela 
Published: November 2020

AbstractForkhead box (Fox) family transcription factors are highly conserved and play essential roles in a wide range of cellular and developmental processes. We report an individual with severe neurological symptoms including postnatal microcephaly, progressive brain atrophy and global developmental delay associated with ade novomissense variant (M280L) in theFOXR1gene. At the protein level, M280L impaired FOXR1 expression and induced a nuclear aggregate phenotype due to protein misfolding and proteolysis. RNAseq and pathway analysis showed that FOXR1 acts as both a transcriptional activator and repressor with central roles in heat shock response, chaperone cofactor-dependent protein refolding and cellular response to stress. Indeed, FOXR1 expression is increased in response to cellular stress, a process in which it directly controlsHSPA6, HSPA1AandDHRS2transcripts. Meanwhile, the ability of the M280L mutant to respond to stress is compromised, in part due to impaired regulation of downstream target genes that are involved in the stress response pathway. Combined, these results suggest FOXR1 plays a role in cellular stress and is necessary for normal brain development.
Directed evolution for improved total secretory protein production inEscherichia coli
Authors: Gonzalez-Perez David , Ratcliffe James , Tan Shu Khan , Wong Mary Chen May , Yee Yi Pei , Nyabadza Natsai , Xu Jian-He , Wong Tuck Seng , Tee Kang Lan 
Published: November 2020

ABSTRACTProduction of secretory protein in Gram-negative bacteria simplifies downstream processing in recombinant protein production, accelerates protein engineering, and advances synthetic biology. Signal peptides and secretory carrier proteins are commonly used to effect the secretion of heterologous recombinant protein in Gram-negative bacteria. TheEscherichia coliosmotically-inducible protein Y (OsmY) is a carrier protein that secretes a target protein extracellularly, and we have successfully applied it in theBacterialExtracellular ProteinSecretionSystem (BENNY) to accelerate the directed evolution workflow. In this study, we applied directed evolution on OsmY to enhance its total secretory protein production.After just one round of directed evolution followed by combining the mutations found, OsmY(M3) (L6P, V43A, S154R, V191E) was identified as the best carrier protein. OsmY(M3) produced 3.1 ± 0.3 fold and 2.9 ± 0.8 fold more secretory Tfu0937 β-glucosidase than its wildtype counterpart inE. colistrains BL21(DE3) and C41(DE3), respectively. OsmY(M3) also produced more secretory Tfu0937 at different cultivation temperatures (37 °C, 30 °C and 25 °C). Subcellular fractionation of the expressed protein confirmed the essential role of OsmY in protein secretion. Up to 80.8 ± 12.2% of total soluble protein was secreted after 15 h of cultivation. When fused to a red fluorescent protein or a lipase fromBacillus subtillis, OsmY(M3) also produced more secretory protein compared to the wildtype.This is the first report of applying directed evolution on a carrier protein to enhance total secretory protein production. The methodology can be further extended to evolve other signal peptides or carrier proteins for secretory protein production inE. coliand other bacteria. In this study, OsmY(M3) improved the production of three proteins, originating from diverse organisms and with diverse properties, in secreted form, clearly demonstrating its wide-ranging applications.
ASPP2/PP1 complexes maintain the integrity of pseudostratified epithelia undergoing remodelling during morphogenesis
Authors: Royer Christophe , Sandham Elizabeth , Slee Elizabeth , Godwin Jonathan , Veits Nisha , Hathrell Holly , Zhou Felix , Leonavicius Karolis , Garratt Jemma , Narendra Tanaya , Vincent Anna , Jones Celine , Child Tim , Coward Kevin , Graham Chris , Lu Xin , Srinivas Shankar 
Published: November 2020

ABSTRACTDuring development, pseudostratified epithelia undergo large scale morphogenetic events associated with increased mechanical stress. The molecular mechanisms that maintain tissue integrity in this context are poorly understood. Using a variety of genetic and imaging approaches, we uncover that the ASPP2/PP1 complex ensures proper epiblast and proamniotic cavity architecture via a mechanism that specifically prevents the most apical daughter cells from delaminating apically following cell division events. The ASPP2/PP1 complex achieves this by maintaining the integrity and organisation of the F-actin cytoskeleton at the apical surface of dividing cells. ASPP2/PP1 is also essential during gastrulation in the primitive streak, in somites and in the head fold region, suggesting that this complex is required across a wide range of pseudostratified epithelia during morphogenetic events that are accompanied by intense tissue remodelling and high cell proliferation. Finally, our study also suggests that the interaction between ASPP2 and PP1 is essential to the tumour suppressor function of ASPP2 which may be particularly relevant in the context of tissues that are subject to increased mechanical stress.
Yeast Nuak1 phosphorylates histone H3 threonine 11 in low glucose stress conditions by the cooperation of AMPK and CK2 signaling
Authors: Oh Seunghee , Lee Jaehyoun , Swanson Selene K. , Florens Laurence , Washburn Michael P. , Workman Jerry L. 
Published: November 2020

AbstractChanges in available nutrients are inevitable events for most living organisms. Upon nutritional stress, several signaling pathways cooperate to change the transcription program through chromatin regulation to rewire cellular metabolism. In budding yeast, histone H3 threonine 11 phosphorylation (H3pT11) acts as a marker of low glucose stress and regulates the transcription of nutritional stress responsive genes. Understanding how this histone modification ‘senses’ external glucose changes remains elusive. Here, we show that Tda1, the yeast orthologue of human Nuak1, is a direct kinase for H3pT11 upon low glucose stress. Yeast AMPK directly phosphorylates Tda1 to govern Tda1 activity, while CK2 regulates Tda1 nuclear localization. Collectively, AMPK and CK2 signaling converge on histone kinase Tda1 to link external low glucose stress to chromatin regulation.
The Y14-p53 Regulatory Circuit in Megakaryocyte Differentiation and Thrombocytopenia
Authors: Su Chun-Hao , Liao Wei-Ju , Ke Wei-Chi , Yang Ruey-Bing , Tarn Woan-Yuh 
Published: November 2020

SUMMARYThrombocytopenia-absent radius syndrome is caused by a deletion in chromosome 1q21.1 intranswithRBM8Amutations in the noncoding regions. We generated megakaryocyte-specificRbm8aknockout (Rbm8aKOMK) mice that exhibited marked thrombocytopenia, internal hemorrhage, and splenomegaly, indicating a disorder of platelet production.Rbm8aKOMKmice accumulated immature megakaryocytes in the bone marrow and spleen. Depletion of Y14/RBM8A in human erythroleukemia (HEL) cells inhibited phorbol ester-induced polyploidy and downregulated the signaling pathways associated with megakaryocyte maturation. Accordingly,Rbm8aKOMKmice had reduced expression of surface glycoproteins on platelets and impaired coagulation. Moreover, p53 level was increased in Y14-depleted HEL cells andRbm8aKOMKmegakaryocytes. Treatment with a p53 inhibitor restoredex vivodifferentiation ofRbm8aKOMKmegakaryocytes and unexpectedly activated Y14 expression in HEL cells. Knockout ofTrp53in part restored the platelet count ofRbm8aKOMKmice. These results indicate that the Y14-p53 circuit plays a critical role in megakaryocyte differentiation and platelet production.
Influence tactics and “second-order” change in schools: case study research of principals' political behaviors and strategies
Authors: Friedman Devora , Berkovich Izhak 
Published: November 2020

PurposePrincipals are considered central in initiating and mobilizing changes in schools; however, their political behaviors in the course of school changes are underexplored. The present research investigated the influence tactics used by school principals to induce teachers to join a process of second-order (deep and wide) change in the school teaching and culture. In specific, the authors were interested to know which influence tactics, principals and staff members considered to be efficient during such a second-order change process.Design/methodology/approachThe study was based on a case study method focusing on four Israeli Jewish state public religious schools participating in the “Routes” program aimed at strengthening religious values in schools. Data collection included semi-structured interviews with principals, teachers with program coordinators responsibilities and teachers in four schools.FindingsThe results indicate that school principals who are considered successful in leading changes display two key influence prototypes: a hybrid type that combines soft and hard influence tactics and a unitype that relies on soft influence tactics.Originality/valueThe research study contributed to the limited knowledge in educational administration on micropolitics and political behaviors in the course of school changes.
Effects of MILA on their stock markets: an empirical analysis on market activity and dynamic correlations
Authors: Muñoz Mendoza Jorge Andrés , Sepúlveda Yelpo Sandra María , Velosos Ramos Carmen Lissette , Delgado Fuentealba Carlos Leandro 
Published: November 2020

PurposeThe purpose of this article is to analyze the effects of the integration process for the Integrated Market of Latin America (MILA) on its stock markets behavior as well as their degree of integration.Design/methodology/approachDaily time series data were used for stock returns, volatility, volume and the number of transactions and securities between August 16, 2007 and December 28, 2018. A DCC-MGARCH model was applied to analyze the impact of MILA on stock market behavior and predict dynamic correlations. A GARCH (1,1) model was used to determine the effect of MILA on co-movements between markets. Finally, a Markov regime switching model was used for robustness analysis.FindingsMILA increased stock market activity in terms of volume, transactions and securities traded. However, it reduced returns and volatility. MILA had significant effects on the dynamic correlations between regional stock markets. After the integration process, the dynamic correlations of returns and volatility were reduced, but those related to volume, transactions and securities traded increased. Mexico's subsequent entry into MILA further reduced market volatility, but it did not have relevant effects on markets' co-movements.Originality/valueThese results are relevant for investors and policymakers. MILA has benefited the markets by promoting stock market activity, reducing risk, creating a margin for diversification and limiting risk contagion between them. These results help to guide investment decisions due to the fact that MILA's benefits in terms of regional diversification would be greater in some markets.
Tax evasion, political/public corruption and increased taxation: evidence from Zimbabwe
Authors: Kurauone Ophias , Kong Yusheng , Mago Stephen , Sun Huaping , Famba Takuriramunashe , Muzamhindo Simbarashe 
Published: November 2020

Purpose
The purpose of this paper is to examine the relationship between tax evasion, political/public corruption and increased taxation in Zimbabwe’s small and medium-sized enterprises (SMEs).

Design/methodology/approach
The study as a descriptive survey used questionnaires and interviews as research instruments for collecting data.

Findings
The findings revealed that most SMEs are no longer paying some form of taxes as expected since the Government of Zimbabwe through the Ministry of Finance and Reserve Bank of Zimbabwe introduced the 2% tax levy on all bank electronic transactions greater than US$10 from October 2018.

Originality/value
The paper recommends that the government should create an independent anti-corruption committee with strong monitoring and regulatory mechanism so as to fight political/public corruption; hence, creating a paradigm of trust and confidence among different economic players. Lastly, the tax authorities should engage all the key economic players when crafting the country’s tax laws/rates so as to promote a sense of equity, equality and economic transparency among citizens.
Entrepreneurial decision-making for global strategies: a “heart–head” approach
Authors: Benevolo Clara , Penco Lara , Torre Teresina 
Published: November 2020

PurposeThe purpose of this study is to build a proposal for an integrated analysis / evaluation / decision / implementation framework to suit entrepreneurial companies intending to redefine their strategic behaviours and their competitive schemes in the global environment.Design/methodology/approachA conceptual approach, based on theoretical perspectives relating to globalisation-based management and decision-making of entrepreneurs, is adopted.FindingsThe paper presents a conceptual framework useful for supporting entrepreneurial decisions for global strategies, drawing on an integrated analysis of external environment and internal components, with a specific focus on the entrepreneur's characteristics in terms of cognitive and emotional profiles. It is suggested to adopt a logical process aimed at identifying which strategic levers are available for entrepreneurial players to implement their strategies.Practical implicationsEntrepreneurial decision-making may benefit from an integrated framework which helps entrepreneurs, who aim to compete in the global marketplace, to explore and exploit all the key factors useful to defining their strategies.Originality/valueThere is a lack of decision-making frameworks that put the entrepreneur at the centre and, at the same time, present a potential balance between the external factors (globalisation drivers and local opportunities) and the resources and competences required to manage risks and difficulties of the global environment (internal factors). The originality of the proposed framework consists in filling this gap. Moreover, this framework can be useful for “re-born global” or “global-again” firms that are currently a neglected typology of studied firms.
When career success enhances employees' life satisfaction: different effects of two types of goal orientations
Authors: Choi Byoung Kwon , Nae Eun Young 
Published: November 2020

PurposeDrawing on goal orientation theory, the authors propose a moderated mediation model, wherein objective career success is positively related to employees' life satisfaction through subjective career success moderated by learning and performance goal orientations.Design/methodology/approachData were collected from 188 employees in South Korea. The hypotheses were tested with the moderated mediation regression analysis.FindingsThe results indicated that salary and promotion, as indicators of objective career success, were positively related to subjective career success. However, subjective career success mediated only the influence of salary, not promotion, on life satisfaction. Furthermore, the authors found that the indirect relationship between salary and life satisfaction via subjective career success was not significant for employees with high learning goal orientation but was significant for those with high performance goal orientation.Practical implicationsOrganizations need to understand that a higher salary and frequent promotions may not always be positively related to employees' satisfaction with career and personal life and should consider the types of goal orientations.Originality/valueThe authors’ consideration of goal orientation as a dispositional characteristic contributes to the comprehensive understanding of how employees' learning and performance goal orientations interact with objective career success in influencing their subjective career and life satisfaction.
A measure of adaptive performance and the DLOQ
Authors: Kim Kyoungshin 
Published: November 2020

PurposeThis study aims to introduce adaptive performance as an organizational performance dimension and examine the possible dynamics between the dimensions of a learning organization and adaptive performance.Design/methodology/approachThis study used a survey and applied factor analysis and structural equation modeling analysis.FindingsThe results supported adaptive performance as an organizational performance dimension. Also, the findings of this study empirically proved that perceived knowledge and adaptive performance mediate the positive relationship between a learning organization and perceived financial performance.Research limitations/implicationsIn addition to the current Dimensions of a Learning Organization Questionnaire (DLOQ) studies, this study revealed that adaptive performance, one of the nonfinancial outcomes improved by learning, had a significant effect on financial performance. Also, this study provided evidence of the additional construct validity of the DLOQ, particularly its performance measures.Practical implicationsThis study advises practitioners to take a close look at how learning and organization development activities improve organizational performance overall.Originality/valueThis study supported a claim that learning and organization development activities in organizations have a strong potential to induce variance in intangible performance.
The involvement of α‐proteobacteria Phenylobacterium in maintaining the dominance of toxic Microcystis blooms in Lake Taihu, China
Authors: Zuo Jun , Hu Lili , Shen Wei , Zeng Jiaying , Li Lin , Song Lirong , Gan Nanqin 
Published: November 2020

SummaryLake Taihu in China has suffered serious harmful cyanobacterial blooms for decades. The algal blooms threaten the ecological sustainability, drinking water safety, and human health. Although the roles of abiotic factors (such as water temperature and nutrient loading) in promoting Microcystis blooms have been well studied, the importance of biotic factors (e.g. bacterial community) in promoting and meditating Microcystis blooms remains unclear. In this study, we investigated the ecological dynamics of bacterial community, the ratio of toxic Microcystis, as well as microcystin in Lake Taihu. High‐throughput 16S rRNA sequencing and principal component analysis (PCA) revealed that the bacteria community compositions (BCCs) clustered into three groups, the partitioning of which corresponded to that of groups according to the toxic profiles (the ratio of toxic Microcystis to total Microcystis, and the microcystin concentrations) of the samples. Further Spearman's correlation network showed that the α‐proteobacteria Phenylobacterium strongly positively correlated with the toxic profiles. Subsequent laboratory chemostats experiments demonstrated that three Phenylobacterium strains promoted the dominance of the toxic Microcystis aeruginosa PCC7806 when co‐culturing with the non‐toxic PCC7806 mcyB− mutant. Taken together, our data suggested that the α‐proteobacteria Phenylobacterium may play a vital role in the maintenance of toxic Microcystis dominance in Lake Taihu.