bluuebunny/crossref_metadata_2025_split huggingface dataset:
A simple monochromatic flow cytometric assay for assessment of intraerythrocytic development of Plasmodium falciparum
Authors: Kulkeaw Kasem , Ketprasit Nutpakal , Tungtrongchitr Anchalee , Palasuwan Duangdao 
Published: February 2020

Abstract
Background
Gold standard microscopic examination of Plasmodium falciparum intraerythrocytic stage remains an important process for staging and enumerating parasitized erythrocytes in culture; however, microscopy is laborious and its accuracy is dependent upon the skill of the examiner.

Methods
In this study, ViSafe Green (VSG), which is a nucleic acid-binding fluorescent dye, was used for assessing in vitro development of P. falciparum using flow cytometry.

Results
Fluorescence intensity of VSG was found to depend on the developmental stage of parasites. Specifically, multiple-nuclei-containing schizonts were observed in the VSGhigh population, and growing trophozoites and ring-shaped forms were observed in the VSGintermediate and VSGlow populations. The efficacy of VSG-based assay was found to be comparable to the microscopic examination method, and it demonstrated an ability to detect as low as 0.001% of the parasitaemia estimated by Giemsa staining. Moreover, when applying VSG for anti-malarial drug test, it was able to observe the growth inhibitory effect of dihydroartemisinin, the front-line drug for malaria therapy.

Conclusions
Taken together, the results of this study suggest the VSG-based flow cytometric assay to be a simple and reliable assay for assessing P. falciparum malaria development in vitro.
Fertility preservation in BRCA mutation carriers—efficacy and safety issues: a review
Authors: Zhang Xiaofu , Niu Jingxin , Che Tuanjie , Zhu Yibei , Zhang Hongtao , Qu Jing 
Published: February 2020

AbstractBRCA mutation carriers face various situations that influence their fertility potential. There is still a lack of guideline or expert consensus on Fertility Preservation (FP) in BRCA mutation carriers and the necessity and safety of FP in BRCA mutation carriers is still in dispute. This review aims to focus on the population of BRCA mutation carriers by analyzing the existing FP strategies, comprehensively comparing the pros and cons of each strategy and its applicability.FP is a suggestion for BRCA mutation carriers with birth planning. Different FP strategies have different characteristics. Considering the particularity of BRCA mutation carriers, multiple factors need to be carefully considered. This review focuses on the applicability of each FP method for carriers under various circumstances. Available FP strategies including oocyte cryopreservation, ovarian tissue cryopreservation, preimplantation genetic diagnosis, and egg/embryo donation are analyzed by comparing existing methods comprehensively. In the attempt to provide an up-to-date decision-making guidance. Conditions taking into consideration were the carrier’s age, the risk of breast and ovarian metastasis, plans for oncotherapy, FP outcome, time available for FP intervention and accessibility.Overall, FP is necessary and safe for BRCA mutation carriers. Among all available FP methods, oocyte cryopreservation is the most reliable procedure; ovarian tissue cryopreservation is the only way for preserving both fertility and endocrine function, recommended for pre-pubertal carriers and when time is limited for oocyte stimulation. A clear framework provides frontline clinical practitioners a new thought and eventually benefit thousands of BRCA mutation carriers.
Conceptualising the initiation of researcher and research user partnerships: a meta-narrative review
Authors: Zych Maria Maddalena , Berta Whitney B. , Gagliardi Anna R. 
Published: February 2020

Abstract
Background
Integrated knowledge translation refers to researcher and research user partnerships to co-generate and implement knowledge. This type of partnership may be critical to success in increasing knowledge use and impact, but the conceptualisation of its initiation has not been fully developed. Initiating this type of partnership has proven to be challenging but crucial to its success. The purpose of this study was to conduct a meta-narrative review of partnership initiation concepts, processes, enablers, barriers and outcomes in the disciplines of healthcare and social sciences where examples of researcher and research user partnerships were found.

Methods
Seven research traditions were identified. Three were in the discipline of social sciences (including psychology, education and business) and five were in the discipline of healthcare (including medicine, nursing, public health, health services research). Searches were conducted in MEDLINE, EMBASE, CINAHL, ABI Inform, ERIC, PsychInfo and the Cochrane Library on June 9, 2017. Fifty titles and abstracts were screened in triplicate; data were extracted from three records in duplicate. Narratives comprised of study characteristics and conceptual and empirical findings across traditions were tabulated, summarised and compared.

Results
A total of 7779 unique results were identified and 17 reviews published from 1998 to 2017 were eligible. All reviews identified a partnership initiation phase referred to as ‘early’ or ‘developmental’, or more vaguely as ‘fuzzy’, across six traditions – integrated knowledge translation, action research, stakeholder engagement, knowledge transfer, team initiation and shared mental models. The partnership initiation processes, enablers, barriers and outcomes were common to multiple narratives and summarised in a Partnership Initiation Conceptual Framework. Our review revealed limited use or generation of theory in most included reviews, and little empirical evidence testing the links between partnership initiation processes, enablers or barriers, and outcomes for the purpose of describing successful researcher and research user partnership initiation.

Conclusions
Narratives across multiple research traditions revealed similar integrated knowledge translation initiation processes, enablers, barriers and outcomes, which were captured in a conceptual framework that can be employed by researchers and research users to study and launch partnerships. While partnership initiation was recognised, it remains vaguely conceptualised despite lengthy research in several fields of study. Ongoing research of partnership initiation is needed to identify or generate relevant theory, and to empirically establish outcomes and the determinants of those outcomes.
Timely initiation of breastfeeding in Zimbabwe: evidence from the demographic and health surveys 1994–2015
Authors: Yaya Sanni , Bishwajit Ghose , Shibre Gebretsadik , Buh Amos 
Published: February 2020

Abstract
Background
Timely initiation of breastfeeding or breastfeeding within 60 min of birth has been shown to be associated with significantly lower risk of infant mortality. The World Health Organization recommends starting breastfeeding within the first hour of birth, yet many women in sub-Saharan Africa do not observe this recommendation. To date, there is limited evidence of timely initiation of breastfeeding for Zimbabwe. Therefore, we undertook this study with the aim of calculating the trend in timely initiation of breastfeeding and to explore the correlates.

Methods
We used five rounds of Zimbabwe Demographic and Health Survey data conducted between 1999 and 2015. Participants were 15,923 mothers currently breastfeeding or who had a childbirth within five years preceding the surveys. Outcome variable was self-reported timing of timely breastfeeding for singleton births which was categorized as early (< 60 min), late (≥ 60 min to < 2 4 h) and very late (≥ 24 h).

Results
Prevalence of timely initiation of breastfeeding was 60.3% (95% Confidence Interval [CI] 57.44, 63.02) in 1999, 66.9% (95% CI 64.32, 69.4) in 2006, 65.8% (95% CI 63.7, 67.8) in 2011 and 58.3% (95% CI 56.3, 60.4) in 2015. It increased by 27 and 22% in 2006 and 2011 compared with that of the 1999 level respectively. We found no socio-economic and gender-based differentials in the prevalence of timely initiation of breastfeeding. Compared with women aged 15–19 years old, women 25–29 and 30–34 years old had higher odds of practicing timely initiation of breastfeeding. The odds of practicing timely initiation of breastfeeding among Muslim women (adjusted odds ratio [aOR] 1.2, 95% CI 1.07, 1.36) was 20% higher when compared with Christian mothers. Women who wanted to have their last child later (aOR 0.89, 95% CI 0.81, 0.97) had 11% lower odd of practicing timely initiation of breastfeeding when compared with women who wanted children then.

Conclusions
The prevalence of timely initiation of breastfeeding in Zimbabwe was 58.3% in 2015, well over the 50% target recommended by WHO for all countries to attain by 2025.
Association between parity and macrosomia in Shaanxi Province of Northwest China
Authors: Lei Fangliang , Zhang Lili , Shen Yuan , Zhao Yaling , Kang Yijun , Qu Pengfei , Mi Baibing , Dang Shaonong , Yan Hong 
Published: February 2020

Abstract
Objective
To explore the relationship between parity and macrosomia and provide the necessary reference for the maternal and children health service.

Method
A cross-sectional epidemiological survey with the purpose to assess the birth outcomes was conducted in Shaanxi province, China.

Results
The incidence of macrosomia in multiparas was higher than that in primiparas. Univariate analysis showed that maternal age < 25 years, peasant/housework, living in rural areas and female infants were the protective factors of macrosomia. The possibility of having a macrosomic infant also increased with gestational age, maternal education level, household wealth index, living in Central Shaanxi and gestational diabetes. The generalized linear mixed models represented the association between parity and macrosomia. After adjusting for statistically significant factors in univariate analysis from model 1 to model 3, the risk of being born macrosomia was 1.26 times higher for a multipara compared to that for a primipara.

Conclusions
Present study indicated parity of two children was associated with increased risk for macrosomic births compared with parity of one child. Compared to primiparas, multiparas should far strengthen the pre-pregnancy education and the guidance during pregnancy to control pre-pregnancy body mass index and pregnancy weight, and keep the appropriate exercise and balanced diet.
Anxiety disorders in acute central nervous system infections
Authors: Bozzola Elena , Spina Giulia , Bergonzini Paola , Bozzola Mauro , Raponi Massimiliano , Villani Alberto 
Published: February 2020

Abstract
Background
Patients affected by acute central nervous system (ACNS) infectionsmay present different complications, including neuropsychological disorders. Nevertheless, psychopathological impairment has been rarely measured by appropriate and validated tests.

Material and methods
Survivors of childhood ACNS infections admitted to the Bambino Gesù Children’s Hospital, Rome, Italy, from June 2013 to June 2015 were re-evaluated at follow-up from June 2016 to June 2017. Both patients and their parents underwent a psychological interview and neuropsychological tests (the Leiter International Performance Scale – revised (Leiter-R), the Child Behaviour Checklist (CBCL), the K-SADS-PL test).

Results
Thirty children were included in the study. The mean score of IQ and fluid reasoning was within the normal range. A percentage of 20% of the children enrolled showed criteria for generalized anxiety disorder.

Conclusion
Our study revealed the importance of follow-up evaluations after ACNS infections, in order to prevent mayor psychological sequelae and to perform treatment or rehabilitation.
Defining persistent critical illness based on growth trajectories in patients with sepsis
Authors: Zhang Zhongheng , Ho Kwok M. , Gu Hongqiu , Hong Yucai , Yu Yunsong 
Published: February 2020

Abstract
Background
Persistent critical illness is common in critically ill patients and is associated with vast medical resource use and poor clinical outcomes. This study aimed to define when patients with sepsis would be stabilized and transitioned to persistent critical illness, and whether such transition time varies between latent classes of patients.

Methods
This was a retrospective cohort study involving sepsis patients in the eICU Collaborative Research Database. Persistent critical illness was defined at the time when acute physiological characteristics were no longer more predictive of in-hospital mortality (i.e., vital status at hospital discharge) than antecedent characteristics. Latent growth mixture modeling was used to identify distinct trajectory classes by using Sequential Organ Failure Assessment score measured during intensive care unit stay as the outcome, and persistent critical illness transition time was explored in each latent class.

Results
The mortality was 16.7% (3828/22,868) in the study cohort. Acute physiological model was no longer more predictive of in-hospital mortality than antecedent characteristics at 15 days after intensive care unit admission in the overall population. Only a minority of the study subjects (n = 643, 2.8%) developed persistent critical illness, but they accounted for 19% (15,834/83,125) and 10% (19,975/198,833) of the total intensive care unit and hospital bed-days, respectively. Five latent classes were identified. Classes 1 and 2 showed increasing Sequential Organ Failure Assessment score over time and transition to persistent critical illness occurred at 16 and 27 days, respectively. The remaining classes showed a steady decline in Sequential Organ Failure Assessment scores and the transition to persistent critical illness occurred between 6 and 8 days. Elevated urea-to-creatinine ratio was a good biochemical signature of persistent critical illness.

Conclusions
While persistent critical illness occurred in a minority of patients with sepsis, it consumed vast medical resources. The transition time differs substantially across latent classes, indicating that the allocation of medical resources should be tailored to different classes of patients.
JMJD1B, a novel player in histone H3 and H4 processing to ensure genome stability
Authors: Saavedra Francisco , Gurard-Levin Zachary A. , Rojas-Villalobos Camila , Vassias Isabelle , Quatrini Raquel , Almouzni Geneviève , Loyola Alejandra 
Published: February 2020

Abstract
Background
Maintaining a proper supply of soluble histones throughout the cell cycle is important to ensure chromatin and genome stability. Following their synthesis, histones undergo a series of maturation steps to prepare them for deposition onto chromatin.

Results
Here, we identify the lysine demethylase JMJD1B as a novel player in the maturation cascade that contributes to regulate histone provision. We find that depletion of JMJD1B increases the protein levels of the histone chaperone tNASP leading to an accumulation of newly synthesized histones H3 and H4 at early steps of the histone maturation cascade, which perturbs chromatin assembly. Furthermore, we find a high rate of JMJD1B mutations in cancer patients, and a correlation with genomic instability.

Conclusions
Our data support a role for JMJD1B in fine-tuning histone supply to maintain genome integrity, opening novel avenues for cancer therapeutics.
Imaging findings and classification of the common and uncommon male breast diseases
Authors: Önder Ömer , Azizova Aynur , Durhan Gamze , Elibol Funda Dinç , Akpınar Meltem Gülsün , Demirkazık Figen 
Published: February 2020

AbstractMale breast hosts various pathological conditions just like “female breast.” However, histo-anatomical diversities with female breast lead to many differences regarding the frequency and presentation of diseases, the radiologic appearance of lesions, the diagnostic algorithm, and malignity features.Radiological modalities may play an important role in evaluating male breast lesions. Although some imaging findings are non-specific, having knowledge of certain imaging characteristics and radiologic patterns is the key to reduce the number of differential diagnoses or to reach an accurate diagnosis.Male breast imaging is mostly based on physical examination and is required for the complaints of palpable mass, breast enlargement, tenderness, nipple discharge, and nipple-skin changes. The majority of the male breast lumps are benign and the most common reason is gynecomastia. Although it is difficult to exclude malignancy in some cases, gynecomastia often has distinguishable imaging features. Pseudogynecomastia is another differential diagnosis that may be confused with gynecomastia. The distinction is important for the treatment plan.Apart from gynecomastia, other male breast lesions form a highly heterogeneous group and can be classified based on “Tissue origin,” “Histopathological type and behavior,” and “Radiologic features” for both simplification and comprehensive understanding.This article mainly focuses on emphasizing the results of basic histo-anatomical differences of male and female breasts, classifying male breast lesions, covering the spectrum of male breast diseases, and assisting radiologists in recognizing the imaging findings, in interpreting them through a holistic approach, in making a differential diagnosis, and in being a part of proper patient management.
High-mobility group box 1 protein (HMGB1) from Cherry Valley duck mediates signaling pathways and antiviral activity
Authors: Hou Xiaolan , Liu Gen , Zhang Huihui , Hu Xiaofang , Zhang Xinyue , Han Fei , Cui Huizhen , Luo Jinjian , Guo Ru , Li Rong , Li Ning , Wei Liangmeng 
Published: February 2020

AbstractHigh-mobility group box 1 protein (HMGB1) shows endogenous damage-associated molecular patterns (DAMPs) and is also an early warning protein that activates the body’s innate immune system. Here, the full-length coding sequence of HMGB1 was cloned from the spleen of Cherry Valley duck and analyzed. We find that duck HMGB1(duHMGB1) is mostly located in the nucleus of duck embryo fibroblast (DEF) cells under normal conditions but released into the cytoplasm after lipopolysaccharide (LPS) stimulation. Knocking-down or overexpressing duHMGB1 had no effect on the baseline apoptosis rate of DEF cells. However, overexpression increased weakly apoptosis after LPS activation. In addition, overexpression strongly activated the IFN-I/IRF7 signaling pathway in DEF cells and significantly increased the transcriptional level of numerous pattern recognition receptors (PRRs), pro-inflammatory cytokines (IL-6, TNF-α), IFNs and antiviral molecules (OAS, PKR, Mx) starting from 48 h post-transfection. Overexpression of duHMGB1 strongly impacted duck virus replication, either by inhibiting it from the first stage of infection for novel duck reovirus (NDRV) and at late stage for duck Tembusu virus (DTMUV) or duck plague virus (DPV), or promoting replication at early stage for DTMUV and DPV infection. Importantly, data from duHMGB1 overexpression and knockdown experiments, time-dependent DEF cells transcriptional immune responses suggest that duHMGB1 and RIG-I receptor might cooperate to promote the expression of antiviral proteins after NDRV infection, as a potential mechanism of duHMGB1-mediated antiviral activity.
New estimations for the Berezin number inequality
Authors: Bakherad Mojtaba , Yamancı Ulas 
Published: February 2020

AbstractIn this paper, by the definition of Berezin number, we present some inequalities involving the operator geometric mean. For instance, it is shown that if $X, Y, Z\in {\mathcal{L}}(\mathcal{H})$X,Y,Z∈L(H) such that X and Y are positive operators, then $$\begin{aligned} \operatorname{ber}^{r} \bigl( ( X\mathbin{\sharp} Y ) Z \bigr) &\leq \operatorname{ber} \biggl(\frac{ ( Z^{\star }YZ ) ^{\frac{rq}{2}}}{q}+ \frac{X^{ \frac{rp}{2}}}{p} \biggr) -\frac{1}{p}\inf_{\lambda \in \varOmega } \bigl( \bigl[ \widetilde{X} ( \lambda ) \bigr] ^{\frac{rp}{4}}- \bigl[ \widetilde{ \bigl( Z^{\star }YZ \bigr) } ( \lambda ) \bigr] ^{ \frac{rq}{4}} \bigr) ^{2}, \end{aligned}$$ berr((X♯Y)Z)≤ber((Z⋆YZ)rq2q+Xrp2p)−1pinfλ∈Ω([X˜(λ)]rp4−[(Z⋆YZ)˜(λ)]rq4)2, in which $X\mathbin{\sharp} Y=X^{\frac{1}{2}} ( X^{-\frac{1}{2}}YX^{- \frac{1}{2}} ) ^{\frac{1}{2}}X^{\frac{1}{2}}$X♯Y=X12(X−12YX−12)12X12, $p\geq q>1$p≥q>1 such that $r\geq \frac{2}{q}$r≥2q and $\frac{1}{p}+\frac{1}{q}=1$1p+1q=1.
Bivariate Bernstein–Schurer–Stancu type GBS operators in $(p,q)$-analogue
Authors: Mursaleen M. , Ahasan Mohd. , Ansari K. J. 
Published: February 2020

AbstractThe purpose of this paper is to construct a$(p,q)$(p,q)-analogue of Bernstein–Schurer–Stancu type GBS (generalized Boolean sum) operators for approximatingB-continuous andB-differentiable functions. We also establish uniform convergence theorem and estimate the degree of approximation ofB-continuous andB-differentiable functions.
Guided web-based treatment program for reducing cannabis use: a randomized controlled trial
Authors: Sinadinovic Kristina , Johansson Magnus , Johansson Ann-Sofie , Lundqvist Thomas , Lindner Philip , Hermansson Ulric 
Published: February 2020

Abstract
Background
The aim of this study was to investigate the effects of a web-based treatment program with therapist guidance for adults and adolescents with regular cannabis use from the general population.

Methods
A double blinded randomized controlled trial with a parallel group design was conducted (intervention group n = 151, wait-list control group n = 152). Follow-up 12 weeks from treatment commencement of a 13-module intervention. The primary outcome was frequency of cannabis use. Time by group interaction effects were modeled using generalized estimated equations and the instrumental variable approach was used to estimate the effect of intervention adherence.

Results
At follow-up, the intention to treat (ITT) analyses did not show any significant time by group effects. A significant association between intervention adherence and scores on the cannabis abuse screening test (CAST) was found. Secondary analysis excluding participants who had received other professional help revealed time by group effects for secondary outcomes gram cannabis consumed past week, number of dependency criteria and CAST score. Due to methodological limitations, these latter results should be interpreted with caution.

Conclusions
In this study we did not find a web-based treatment program with therapist guidance to be more effective than a waiting-list in reducing frequency of cannabis use.
Trial registration The trial was pre-registered at ClinicalTrials.gov (NCT02408640) April 3, 2015