bluuebunny/crossref_metadata_2025_split huggingface dataset:
Health professionals’ experiences of providing care for women survivors of sexual violence in psychiatric inpatient units
Authors: O’Dwyer Carol , Tarzia Laura , Fernbacher Sabin , Hegarty Kelsey 
Published: November 2019

Abstract
Background
Survivors of sexual violence, who are predominantly women, commonly access mental health services. Psychiatric inpatient units in Australia are predominately mixed gender and may further retraumatise these women. Sexual violence is under-recognised by mental health professionals and there is a lack of adequate policy or direction for mental health service services. To date, only a small amount of research has focused on health professionals’ experiences of providing trauma-informed care to women in psychiatric settings, with most studies focused on specific practices or interventions. Qualitative data is particularly lacking on this topic. This is a critical gap in the knowledge given that health professionals are key to detecting and addressing victimisation. The aim of this study was to gain an in-depth understanding of healthcare professionals’ experiences and perceptions in providing care to women who are survivors of sexual violence in psychiatric inpatient units.

Methods
This qualitative study utilised semi-structured interviews with 40 health professionals recruited from four psychiatric inpatient units within a large Australian public mental health organisation. Data were examined using thematic analysis.

Results
Three main typologies were developed to describe participants’ experiences of the care provided to women; 1) Dismissing and denying; 2) Acknowledging but unprepared; 3) Empathising but despairing.

Discussion
Gender, professional training, adherence to the biomedical model, and level of experience influenced health professionals’ experiences.

Conclusions
Health professionals in this study held varying attitudes towards female consumers and responses to sexual violence. Our findings suggest the need to address individual staff perception and promote trauma-informed and gender-sensitive care across all disciplines, genders, and levels of experience.
MicroRNA-346 inhibits the growth of glioma by directly targeting NFIB
Authors: Li Yangyang , Xu Jia , Zhang Jiale , Zhang Jie , Zhang Jian , Lu Xiaoming 
Published: November 2019

Abstract
Background
Glioma is considered one of the most common tumors and has a poor prognosis. Recently, microRNAs (miRNAs) have been reported to be strongly linked to various human tumors including glioma. In this study, we investigated a new anticancer miRNA, miR-346, to determine the effects and mechanism of miR-346 and its downstream target gene NFIB on tumors.

Methods
Lentivirus transfection, real-time PCR, western blotting, immunohistochemistry, cell proliferation assays, and mouse experiments were used to examine the relationship between miR-346 and its regulation of NFIB in glioma cells.

Results
The expression of miR-346 was downregulated in glioma cells. Overexpression of miR-346 arrested the cell cycle of glioma cells and inhibited their proliferation in vitro and in vivo. NFIB was a direct target of miR-346, whose expression was reduced by the miRNA. Overexpression of NFIB reversed all tested functions of miR-346.

Conclusion
miR-346 inhibited the growth of glioma cells by targeting NFIB and may be a new prognostic and diagnostic biomarker for glioma.
circGSK3β promotes metastasis in esophageal squamous cell carcinoma by augmenting β-catenin signaling
Authors: Hu Xueting , Wu Duoguang , He Xiaotian , Zhao Huiying , He Zhanghai , Lin Jiatong , Wang Kefeng , Wang Wenjian , Pan Zihao , Lin Huayue , Wang Minghui 
Published: November 2019

Abstract
Background
Circular RNAs (circRNAs), a novel class of noncoding RNAs, have recently drawn much attention in the pathogenesis of human cancers. However, the role of circRNAs in esophageal squamous cell carcinoma (ESCC) remains unclear. In this study, we aimed to identify novel circRNAs that regulate ESCC progression and explored their regulatory mechanisms and clinical significance in ESCC.

Methods
Differentially expressed circRNAs between ESCC and paired adjacent normal tissues were identified using microarrays. The effects of a specific differentially expressed circRNA (circGSK3β) on tumor progression were explored in vitro and in vivo. Plasma samples from patients with ESCC, benign lesions and healthy controls were subjected to droplet digital PCR (ddPCR) analyses for circGSK3β, and the detection rates of plasma circGSK3β for ESCC were investigated.

Results
We demonstrated that upregulated expression of circGSK3β was positively associated with advanced clinical stage and poor outcome in patients with ESCC. We further revealed that circGSK3β promoted ESCC cell migration and invasion via direct interaction with GSK3β and inhibiting GSK3β activity, providing a novel mechanism of circRNA in cancer progression. Importantly, we identified that circGSK3β expression in plasma was a biomarker for detection of ESCC and early stage of ESCC with the area under curve (AUC) of 0.782 and 0.793, respectively.

Conclusions
CircGSK3β exerts critical roles in promoting ESCC metastasis and may serve as a novel therapeutic target for ESCC patients. The plasma level of circGSK3β have potential to serve as a novel diagnostic and prognostic biomarker for ESCC detection.
Circulating microRNAs as minimal residual disease biomarkers in childhood acute lymphoblastic leukemia
Authors: Rzepiel Andrea , Kutszegi Nóra , Gézsi András , Sági Judit C. , Egyed Bálint , Péter György , Butz Henriett , Nyírő Gábor , Müller Judit , Kovács Gábor T. , Szalai Csaba , Semsei Ágnes F. , Erdélyi Dániel J. 
Published: November 2019

Abstract
Background
Treatment stratification based on bone marrow minimal residual disease (MRD) at set time points has resulted in considerably improved survival in pediatric acute lymphoblastic leukemia (ALL). Treatment response is assessed using bone marrow samples. MicroRNAs (miRs) easily traffic among fluid spaces and are more stable than most other RNA classes. We examined the role of circulating miRs as putative less invasive MRD biomarkers.

Methods
In an exploratory experiment, expression of 46 preselected miRs was studied in platelet-free blood plasma samples of 15 de novo, 5 relapsed ALL patients and 10 controls by Custom TaqMan Array Advanced MicroRNA Card. Based on their high expression in ALL compared to controls, and on the reduction observed along the induction therapy, four miRs were selected for further analyses: miR-128-3p, -181a-5p, -181b-5p and 222-3p. Their expression was measured by qPCR at 4 time points in 27 de novo ALL patients treated in the ALL IC-BFM 2009 study.

Results
The expression of all 4 miRs significantly decreased over the first week of therapy (miR-128-3p: log2 fold change − 2.86; adjusted p 3.6 × 10−7; miR-181b-5p: log2 fold change − 1.75; adjusted p 1.48 × 10−2; miR-181a-5p: log2 fold change -1.33; adjusted p 3.12 × 10−2; miR-222-3p: log2 fold change − 1.25; adjusted p 1.66 × 10−2). However, no significant further reduction in miR expression was found after the 8th day of therapy. Measured drop in expression of 2 miRs at day 8 strongly correlated with day 15 bone marrow flow cytometry MRD results (miR-128-3p: Pearson’s r = 0.88, adjusted p = 2.71 × 10−4; miR-222-3p: r = 0.81, adjusted p = 2.99 × 10−3).

Conclusion
In conclusion, these circulating miRs might act as biomarkers of residual leukemia. MiR-128-3p and miR-222-3p in blood predict day 15 flow cytometry MRD results 7 days earlier. Although, their sensitivity falls behind that of bone marrow flow cytometry MRD at day 15.
Actionability of HER2-amplified circulating tumor cells in HER2-negative metastatic breast cancer: the CirCe T-DM1 trial
Authors: Jacot William , Cottu Paul , Berger Frederique , Dubot Coraline , Venat-Bouvet Laurence , Lortholary Alain , Bourgeois Hugues , Bollet Marc , Servent Veronique , Luporsi Elisabeth , Espié Marc , Guiu Severine , D’Hondt Veronique , Dieras Veronique , Sablin Marie-Paule , Brain Etienne , Neffati Souhir , Pierga Jean-Yves , Bidard Francois-Clement 
Published: November 2019

AbstractBackgroundIn this prospective phase 2 trial, we assessed the efficacy of trastuzumab-emtansine (T-DM1) in HER2-negative metastatic breast cancer (MBC) patients with HER2-positive CTC.MethodsMain inclusion criteria for screening were as follows: women with HER2-negative MBC treated with ≥ 2 prior lines of chemotherapy and measurable disease. CTC with aHER2/CEP17 ratio of ≥ 2.2 by fluorescent in situ hybridization (CellSearch) were considered to beHER2-amplified (HER2amp). Patients with ≥ 1HER2ampCTC were eligible for the treatment phase (T-DM1 monotherapy). The primary endpoint was the overall response rate.ResultsIn 154 screened patients, ≥ 1 and ≥ 5 CTC/7.5 ml of blood were detected inN = 118 (78.7%) andN = 86 (57.3%) patients, respectively. ≥1HER2ampCTC was found in 14 patients (9.1% of patients with ≥ 1 CTC/7.5 ml). Among 11 patients treated with T-DM1, one achieved a confirmed partial response. Four patients had a stable disease as best response. Median PFS was 4.8 months while median OS was 9.5 months.ConclusionsCTC withHER2amplification can be detected in a limited subset of HER2-negative MBC patients. Treatment with T-DM1 achieved a partial response in only one patient.Trial registrationNCT01975142, Registered 03 November 2013
Associations of race and ethnicity with risk of developing invasive breast cancer after lobular carcinoma in situ
Authors: Dania Vanessa , Liu Ying , Ademuyiwa Foluso , Weber Jason D. , Colditz Graham A. 
Published: November 2019

Abstract
Background
Lobular carcinoma in situ (LCIS) of the breast is a risk factor of developing invasive breast cancer. We evaluated the racial differences in the risks of subsequent invasive breast cancer following LCIS.

Methods
We utilized data from the Surveillance, Epidemiology, and End Results registries to identify 18,835 women diagnosed with LCIS from 1990 to 2015. Cox proportional hazards regression was used to estimate race/ethnicity-associated hazard ratios (HRs) and corresponding 95% confidence intervals (CIs) of subsequent invasive breast cancer.

Results
During a median follow-up of 90 months, 1567 patients developed invasive breast cancer. The 10-year incidence was 7.9% for Asians, 8.2% for Hispanics, 9.3% for whites, and 11.2% for blacks (P = 0.046). Compared to white women, black women had significantly elevated risks of subsequent invasive breast cancer (HR 1.33; 95% CI 1.11, 1.59), and invasive cancer in the ipsilateral breast (HR 1.37; 95% CI 1.08, 1.72) and in the contralateral breast (HR 1.33; 95% CI 1.00, 1.76). Black women had significantly higher risks of invasive subtypes negative for both estrogen receptor and progesterone receptor (HR 1.86; 95% CI 1.14, 3.03) and invasive subtypes positive for one or both of receptors (HR 1.30; 95% CI 1.07, 1.59). The risk of subsequent invasive breast cancer was comparable in Asian women and Hispanic women compared with white women.

Conclusions
Black women had a significantly higher risk of developing invasive breast cancer, including both hormone receptor-positive and hormone receptor-negative subtypes, after LCIS compared with white counterparts. It provides an opportunity to address health disparities.
Differential co-expression-based detection of conditional relationships in transcriptional data: comparative analysis and application to breast cancer
Authors: Bhuva Dharmesh D. , Cursons Joseph , Smyth Gordon K. , Davis Melissa J. 
Published: November 2019

AbstractBackgroundElucidation of regulatory networks, including identification of regulatory mechanisms specific to a given biological context, is a key aim in systems biology. This has motivated the move from co-expression to differential co-expression analysis and numerous methods have been developed subsequently to address this task; however, evaluation of methods and interpretation of the resulting networks has been hindered by the lack of known context-specific regulatory interactions.ResultsIn this study, we develop a simulator based on dynamical systems modelling capable of simulating differential co-expression patterns. With the simulator and an evaluation framework, we benchmark and characterise the performance of inference methods. Defining three different levels of “true” networks for each simulation, we show that accurate inference of causation is difficult for all methods, compared to inference of associations. We show that az-score-based method has the best general performance. Further, analysis of simulation parameters reveals five network and simulation properties that explained the performance of methods. The evaluation framework and inference methods used in this study are available in the dcanr R/Bioconductor package.ConclusionsOur analysis of networks inferred from simulated data show that hub nodes are more likely to be differentially regulated targets than transcription factors. Based on this observation, we propose an interpretation of the inferred differential network that can reconstruct a putative causal network.
European Network of Pregnancy Registers in Rheumatology (EuNeP)—an overview of procedures and data collection
Authors: Meissner Yvette , Strangfeld Anja , Costedoat-Chalumeau Nathalie , Förger Frauke , Goll Doreen , Molto Anna , Özdemir Rebecca , Wallenius Marianne , Fischer-Betz Rebecca 
Published: November 2019

Abstract
Background
The collaborative initiative of the European Network of Pregnancy Registers in Rheumatology (EuNeP) aims to combine data available in nationwide pregnancy registers to increase knowledge on pregnancy outcomes in women with inflammatory rheumatic diseases (IRD) and on drug safety during pregnancy and lactation. The objective of this study was to describe the similarities and differences of the member registers.

Methods
From all registers, information about their structure and design was collected, as well as which parameters regarding demographics, maternal outcomes, treatment, course and outcome of pregnancy, and development of the child were available in the respective datasets. Furthermore, the current recruitment status was reported.

Results
The four registers (EGR2 (France), RePreg (Switzerland), RevNatus (Norway), and Rhekiss (Germany)) collect information prospectively and nationwide. Patients can be enrolled before conception or during pregnancy. To date, more than 3500 patients in total have been included, and data on 2200 pregnancies with an outcome are available. The distribution of diagnoses in the respective registers varies considerably, and only three entities (rheumatoid arthritis, psoriatic arthritis, and spondyloarthritis) are captured by all the registers. Broad consistency was found in non-disease-specific data items, but differences regarding instruments and categories as well as frequency of data collection were revealed. Disease-specific data items are less homogeneously collected.

Conclusion
Although the registers in this collaboration have similar designs, we found numerous differences in the variables collected. This survey of the status quo of current pregnancy registers is the first step towards identifying data collected uniformly across registers in order to facilitate joint analyses.

Trial registration
Not applicable.
Genotypic diversity of Streptococcus suis and the S. suis-like bacterium Streptococcus ruminantium in ruminants
Authors: Okura Masatoshi , Maruyama Fumito , Ota Atsushi , Tanaka Takeshi , Matoba Yohei , Osawa Aya , Sadaat Sayed Mushtaq , Osaki Makoto , Toyoda Atsushi , Ogura Yoshitoshi , Hayashi Tetsuya , Takamatsu Daisuke 
Published: November 2019

AbstractAlthough Streptococcus suis has attracted public attention as a major swine and human pathogen, this bacterium has also been isolated from other animals, including ruminants. However, recent taxonomic studies revealed the existence of other species that were previously identified as S. suis, and some of these isolates were reclassified as the novel species Streptococcus ruminantium. In Japan, biochemically identified S. suis is frequently isolated from diseased ruminants; however, such isolates have not yet been identified accurately, and their aetiological importance in ruminants is unclear. Therefore, to understand the importance of S. suis and S. suis-like bacteria in ruminants, we reclassified S. suis isolates from ruminants according to the updated classification and investigated their genetic diversity. Although both S. suis and S. ruminantium were isolated from healthy and diseased ruminants, most of the isolates from diseased animals were S. ruminantium, implying that S. ruminantium is more likely to be associated with ruminant disease than S. suis. However, the ruminant S. suis and S. ruminantium isolates from diseased animals were classified into diverse genotypes rather than belonging to certain clonal groups. Genome sequence analysis of 20 S. ruminantium isolates provided information about the antibiotic resistance, potential virulence, and serological diversity of this species. We further developed an S. ruminantium-specific PCR assay to aid in the identification of this bacterium. The information obtained and the method established in this study will contribute to the accurate diagnosis of ruminant streptococcal infections.
Idiopathic facial lipoatrophy in a healthy middle-aged woman: A case report
Authors: Purcell Chad , Taylor S. Mark 
Published: November 2019

Background Facial lipoatrophy is a rare condition described by the disappearance of facial subcutaneous fat. The etiology of lipoatrophy can be congenital, or acquired including traumatic, iatrogenic or idiopathic. Idiopathic facial lipoatrophy has only been previously identified in three case reports, among which, the patient demographics vary considerably. Two of these case reports have identified a role for autologous fat transfer as a means of treatment. This case differs from those in the literature in patient demographics and severity of the facial lipoatrophy. The aim of the current report is to present a rare case of idiopathic facial lipoatrophy, and to assess the use of autologous fat transfer as a treatment modality. Case presentation We present a case of a 40-year old woman from Nova Scotia, Canada who presented with asymptomatic idiopathic facial lipoatrophy. The patient was otherwise healthy, taking no medications with no trauma or surgery in the region affected. Investigations, including a full autoimmune workup, were unremarkable. The facial lipoatrophy was considerably disfiguring and was assessed as a Grade 4 on the facial lipoatrophy scale. The patient was treated over the course of 2 years with two autologous fat transfers. Conclusions Achieving resolution of idiopathic lipoatrophy is important to patients because it can manifest in a disfiguring form and have negative effects on quality of life. The current study reports a treatment of idiopathic lipoatrophy that achieved results acceptable to the patient.
Análise da incidência de baciloscopia reagente em presidiários do município pernambucano Canhotinho
Authors: Simonetti Ana Catarina , De Lima Emanoel Mendonça , Cabral Lais Pedrosa Morais , Florêncio Renata Pinheiro 
Published: November 2019

A tuberculose (TB) ainda é uma doença infectocontagiosa de caráter fatídico, pois segundo dados da Organização Mundial de Saúde/OMS (2016), o Brasil é um dos 20 países com elevada prevalência dessa enfermidade, conferindo uma incidência cerca de 50 vezes superior à população geral, nas prisões, assim gerando um maior risco de disseminação da TB. O objetivo dessa pesquisa foi analisar a incidência de casos de baciloscopia reagente em presidiários do município Canhotinho, com base nos dados da V GERES de Garanhuns, Pernambuco. Trata-se de um estudo epidemiológico, descritivo documental retrospectivo, com abordagem quantitativa não experimental, em que prontuários de presidiários (n = 734), concedidos pela V GERES, foram avaliados. Identificou-se que, apenas 14 (1,9%) obtiveram positividade no teste de BK e, consequentemente, 720 (98,1%), obtiveram resultado negativo. No ano de 2016, a positividade representou um aumento de 150% em relação ao ano de 2015, ou seja, 10 (1,4%) casos de positividade foram averiguados, frente 4 (0,5%) casos, em relação ao número total de prontuários. Confere-se que a implementação de políticas públicas mais rígidas, voltadas para a saúde e controle da TB nos presídios é necessária.
A Morse Lemma for Degenerate Critical Points of Solutions of Nonlinear Equations in ℝ2
Authors: Grossi Massimo 
Published: November 2019

Abstract
In this paper we prove a Morse Lemma for degenerate critical points of a function u which satisfies

-

Δ
⁢
u

=

f
⁢

(
u
)

in
⁢

B
1

,

-\Delta u=f(u)\quad\text{in }B_{1},

where

u
∈

C
2

⁢

(

B
1

)

{u\in C^{2}(B_{1})}

,

B
1

{B_{1}}

is the unit ball of

ℝ
2

{\mathbb{R}^{2}}

and f is a smooth nonlinearity. Other results on the nondegeneracy of the critical points and the shape of the level sets are proved.
Effect of Electrospinning Process on Total Antioxidant Activity of Electrospun Nanofibers Containing Grape Seed Extract
Authors: Faki Rabia , Gursoy Oguz , Yilmaz Yusuf 
Published: November 2019

AbstractElectrospinning is a common technique used for the production of nanofibers, and it is based on the fact that the electrically charged liquid polymer is positioned in a continuous fiber form on a grounded surface. Grape seed is rich in phenolic compounds and can be used as a dietary supplement or as a natural antioxidant source in diet. In this study, grape seed extract of Burdur Dimrit variety (Vitis vinifera L.) was electrospun with gelatin, polyvinyl alcohol (PVA) and PVA/β-cyclodextrin polymers to produce nanofibers with antioxidant activity. The aim of this study was to determine the effect of the electrospinning process on the total antioxidant activity and total phenolic contents of electrospun polymers with grape seed extracts. Total antioxidant activity of samples (by ABTS and DPPH assays) and total phenolic contents (Folin–Ciocalteu method) were determined before and after the electrospinning process of polymers with grape seed extract. Electrospinning with gelatin polymer decreased the antioxidant activity (ABTS assay) of nanofibers containing grape seed extract by 65% and their total phenolic contents by 7%. However, electrospinning treatment with PVA and PVA/β-cyclodextrin had no effect on the total antioxidant activity (ABTS and DPPH) and total phenolic substance contents of grape seed extract nanofibers.
Review of large-pore mesostructured cellular foam (MCF) silica and its applications
Authors: Hermida Lilis , Agustian Joni , Abdullah Ahmad Zuhairi , Mohamed Abdul Rahman 
Published: November 2019

AbstractThe unique properties of mesostructured cellular foam (MCF) silica such as, large pore size, continuous three-dimensional (3D) pore system and hydrothermal robust materialallow favorable conditions for incorporating active sites to produce modified MCF silica as catalysts, biocatalysts and adsorbents. Recently, the modified MCF silicas were reported to be efficient catalysts for the hydrogenation of phenylacetylene, heck coupling reaction of arylboronic acid, etc. Biocatalysts derived from modified MCF silicas were found to be a potential to convert glucose to gluconic acid, hydrolysis of N-benzoyl-DLarginine-p-nitroanilide (BAPNA) and casein, transesterification of racemic 1 phenyl- ethanol and hydrolytic, etc. Several separation processes such as CO2 capture and adsorption of L-tryptophan, lysozyme and bovine serum were sucessfully conducted using adsorbents derived from modified MCF silicas. This paper reviews the synthesis of the MCF silica material and the incorporation of active sites or immobilization of enzymes in the MCF silica material. Additionally, a detailed understanding of the characterization of the modified MCF silicas, which includes pore size, active sites/enzymes sizes, amount of active sites/enzymes bound with the MCF silica, was also discussed to obtain their potentialities as catalysts, biocatalysts and adsorbents. The review also describes recent progress on the applications of the MCF silica.