bluuebunny/crossref_metadata_2025_split huggingface dataset:
Gastroesophageal reflux disease after peroral endoscopic myotomy: lest we forget what we already know
Authors: Bechara Robert , Inoue Haruhiro , Shimamura Yuto , Reed David 
Published: January 2020

Summary
After the performance of the first peroral endoscopic myotomy (POEM) in 2008, POEM has now spread worldwide and has arguably become a first-line treatment option for achalasia. Recently, there is increasing debate regarding post-POEM gastroesophageal reflux (GER) and gastroesophageal reflux disease (GERD). The reported incidences of GERD vary widely, owing to the variability in the definitions used. The literature regarding GERD and achalasia patients with a focus on 24-hour pH testing, esophageal acid exposure, and fermentation and the definitions of GERD used in the POEM literature are examined. 24-hour pH testing in achalasia patients may be abnormal due to fermentation both pre- and post-treatment. It is vital that POEM operators ensure that fermentation is recognized during 24-hour pH testing and excluded in the analysis of acid exposure time (AET) used in the diagnosis of GERD. In untreated achalasia, 24-hour pH testing may suggest abnormal AET in over a third of patients. However, most abnormal AETs in untreated achalasia patients are due to fermentation rather than GER. In treated achalasia, up to half of the patients with abnormal AET may be attributable to fermentation. To have a candid discussion and appropriately address the questions surrounding post-POEM GERD, consistent definitions need to be applied. We suggest the recent definition of GERD from the Lyon Consensus to be utilized when diagnosing GERD in post-POEM patients. Further studies are required in establishing ideal parameters for 24-hour pH testing in achalasia patients.
Physical activity, cardiorespiratory fitness, and cardiovascular outcomes in individuals with atrial fibrillation: the HUNT study
Authors: Garnvik Lars E , Malmo Vegard , Janszky Imre , Ellekjær Hanne , Wisløff Ulrik , Loennechen Jan P , Nes Bjarne M 
Published: January 2020

AbstractAimsAtrial fibrillation (AF) confers higher risk of mortality and morbidity, but the long-term impact of physical activity (PA) and cardiorespiratory fitness (CRF) on outcomes in AF patients is unknown. We, therefore, examined the prospective associations of PA and estimated CRF (eCRF) with all-cause mortality, cardiovascular disease (CVD) mortality, morbidity and stroke in individuals with AF.Methods and resultsWe followed 1117 AF patients from the HUNT3 study in 2006–08 until first occurrence of the outcomes or end of follow-up in November 2015. We used Cox proportional hazard regression to examine the prospective associations of self-reported PA and eCRF with the outcomes. Atrial fibrillation patients meeting PA guidelines had lower risk of all-cause [hazard ratio (HR) 0.55, 95% confidence interval (CI) 0.41–0.75] and CVD mortality (HR 0.54, 95% CI 0.34–0.86) compared with inactive patients. The respective HRs for CVD morbidity and stroke were 0.78 (95% CI 0.58–1.04) and 0.70 (95% CI 0.42–1.15). Each 1-metabolic equivalent task (MET) higher eCRF was associated with a lower risk of all-cause (HR 0.88, 95% CI 0.81–0.95), CVD mortality (HR 0.85, 95% CI 0.76–0.95), and morbidity (HR 0.88, 95% CI 0.82–0.95).ConclusionHigher PA and CRF are associated with lower long-term risk of CVD and all-cause mortality in individuals with AF. The findings support a role for regular PA and improved CRF in AF patients, in order to combat the elevated risk for mortality and morbidity.
Mental illness stigma after a decade of Time to Change England: inequalities as targets for further improvement
Authors: Henderson Claire , Potts Laura , Robinson Emily J 
Published: January 2020

Abstract

Background
England’s Time to Change programme to reduce mental health-related stigma and discrimination included a social marketing campaign using traditional and social media, and targeted middle-income groups aged 25–45 between 2009 and 2016. From 2017, the same age group on low to middle incomes were targeted, and the content focused on men’s mental health, by changing the advertising and adapting the ‘key messages’. This study investigates changes in stigma-related public knowledge, attitudes and desire for social distance in England since Time to Change began in 2008–19 and for 2017–19.

Methods
Using data from a face-to-face survey of a nationally representative quota sample of adults for England, we evaluated longitudinal trends in outcomes with regression analyses and made assumptions based on a simple random sample. The pre-existing survey used a measure of attitudes; measures of knowledge and desire for social distance were added in 2009.

Results
Reported in standard deviation units (95% CI), the improvement for knowledge for 2009–19 was 0.25 (0.19, 0.32); for attitudes, 2008–19, 0.32 (0.26, 0.39) and for desire for social distance, 2009–19 0.29 (0.23, 0.36). Significant interactions between year and both region and age suggest greater improvements in London, where stigma is higher, and narrowing of age differences. There were significant improvements between 2017 and 2019 in knowledge [0.09 (0.02, 0.16)] and attitudes [0.08 (0.02, 0.14)] but not social distance.

Conclusion
The positive changes support the effectiveness of Time to Change but cannot be definitively attributed to it. Inequalities in stigma by demographic characteristics present targets for research and intervention.
Improving Alzheimer's disease by altering gut microbiota in tree shrews with ginsenoside Rg1
Authors: Wang Limei , Lu Jiangli , Zeng Yueqin , Guo Yuqian , Wu Chao , Zhao Hongbin , Zheng Hong , Jiao Jianlin 
Published: January 2020

ABSTRACT
Ginsenoside Rg1 (GRg1) has neuroprotective effects on Alzheimer's disease (AD). The occurrence and progression of AD are closely related to gut microbiota. Few studies have learned the direct relationship between GRg1 and gut microbiota. In this study, we found an original way to research this relationship by using GRg1 in the AD model of tree shrews. Morris water maze and immunohistochemistry were performed to test the cognition repairing function of GRg1 by tree shrews and 16S ribosomal RNA sequencing was used to explore the composition and abundance of gut microbiota. After GRg1 treatment, the result of Morris water maze showed an improvement in cognitive function, and immunohistochemistry revealed a decrease in tau protein. Moreover, 16SrRNA sequencing results showed the abundances of Proteobacteria and Verrucomicrobia were significantly different, and Lactobacillaceae was significantly increased in the GRg1 treatment group. It also showed that the gut microbiome with middle and high doses of GRg1 was close to the normal group. In conclusion, this study suggests that GRg1 at middle and high doses may change the abundance of gut microbiota to improve AD, and thatProteobacteria and Verrucomicrobia are key microbiota. This is the first report that has ever studied the relationship between GRg1 and gut microbiota in tree shrews.
Trends and drivers of marine fish landings in Portugal since its entrance in the European Union
Authors: Bueno-Pardo Juan , Pierce Graham J , Cabecinha Edna , Grilo Catarina , Assis Jorge , Valavanis Vasilis , Pita Cristina , Dubert Jesús , Leitão Francisco , Queiroga Henrique 
Published: January 2020

Abstract
Marine landings in Portugal have decreased at a higher rate than fishing effort in the last 20 years. Identifying the variables driving the quantity and composition of landings is pivotal to understand the dynamics of the fisheries sector, which entails complex social and environmental aspects. In this study, we investigate the main drivers of marine fish landings in continental Portugal between 1989 and 2014. To identify common trends in time series, and quantify the importance of environmental factors, we applied a dynamic factor analysis considering four regions and three types of gear (trawling, purse-seine, and a multi-gear fishery). Our results show the importance of fishing effort as the most relevant factor driving marine landings in Portugal, both at the long and short terms. In addition, the effect of environmental factors such as the winter river discharge and the spring East Atlantic Teleconnection index should not be neglected, probably through mechanisms affecting coastal productivity. We provide a comprehensive amount of information that permits to improve our understanding of the trends of the most important commercial species in Portugal during the period of study.
A review of two beliefs of parliamentary government in postwar Japan: Japanese/French beliefs beneath the British canopy
Authors: Kobori Masahiro 
Published: January 2020

Abstract
Although the 1947 Constitution of Japan was enacted under the occupation of the General Headquarters of the Allied Powers, it is thought that a British-style parliamentary government was introduced in Japan at this time. Leading constitutional scholars in postwar Japan believed in two ideas derived from the British constitutional history: (i) monistic parliamentary government and (ii) the free exercise of dissolution power by the British cabinet. Both of these ideas have been widely shared among constitutional scholars in Japan. However, in fact, these two beliefs derived from French scholars, and British constitutional scholars did not share them. The difference in substance and approach between the Japanese/French and the British interpretations was never explored in Japan. Japanese scholars sought a rational understanding of the British parliamentary government, thereby overlooking the empiricist understanding.
Disturbance of Oxidative Stress Parameters in Treatment-Resistant Bipolar Disorder and Their Association With Electroconvulsive Therapy Response
Authors: Lv Qinyu , Hu Qiongyue , Zhang Wenzhong , Huang Xinxin , Zhu Minghuan , Geng Ruijie , Cheng Xiaoyan , Bao Chenxi , Wang Yingyi , Zhang Chen , He Yongguang , Li Zezhi , Yi Zhenghui 
Published: January 2020

Abstract

Objective
Electroconvulsive therapy (ECT) is an effective option for treatment-resistant bipolar disorder (trBD). However, the mechanisms of its effect are unknown. Oxidative stress is thought to be involved in the underpinnings of BD. Our study is the first, to our knowledge, to report the association between notable oxidative stress parameters (superoxide dismutase [SOD], glutathione peroxidase [GSH-Px], catalase [CAT], and malondialdehyde [MDA]) levels and ECT response in trBD patients.

Methods
A total 28 trBD patients and 49 controls were recruited. Six-week ECT and naturalistic follow-up were conducted. SOD, GSH-Px, CAT, and MDA levels were measured by enzyme-linked immunosorbent assay, and the 17-item Hamilton Depression Rating Scale and Young Mania Rating Scale were administered at baseline and the end of the 6th week. MANCOVA, ANCOVA, 2 × 2 ANCOVA, and a multiple regression model were conducted.

Results
SOD levels were lower in both trBD mania and depression (P = .001; P = .001), while GSH-Px (P = .01; P = .001) and MDA (P = .001; P = .001) were higher in both trBD mania and depression compared with controls. CAT levels were positively associated with 17-item Hamilton Depression Rating Scale scores in trBD depression (radjusted  = 0.83, P = .005). MDA levels in trBD decreased after 6 weeks of ECT (P = .001). Interestingly, MDA levels decreased in responders (P = .001) but not in nonresponders (P &gt; .05).

Conclusions
Our study indicates that decreased SOD could be a trait rather than a state in trBD. Oxidative stress levels are associated with illness severity and ECT response. This suggests that the mechanism of oxidative stress plays a crucial role in the pathophysiology of trBD.
A quasi-optimal variant of the hybrid high-order method for elliptic partial differential equations with H−1 loads
Authors: Ern Alexandre , Zanotti Pietro 
Published: January 2020

Abstract
Hybrid high-order (HHO) methods for elliptic diffusion problems have been originally formulated for loads in the Lebesgue space $L^2(\varOmega )$. In this paper we devise and analyse a variant thereof, which is defined for any load in the dual Sobolev space $H^{-1}(\varOmega )$. The main feature of the present variant is that its $H^1$-norm error can be bounded only in terms of the $H^1$-norm best error in a space of broken polynomials. We establish this estimate with the help of recent results on the quasi-optimality of nonconforming methods. We prove also an improved error bound in the $L^2$-norm by duality. Compared to previous works on quasi-optimal nonconforming methods the main novelties are that HHO methods handle pairs of unknowns and not a single function and, more crucially, that these methods employ a reconstruction that is one polynomial degree higher than the discrete unknowns. The proposed modification affects only the formulation of the discrete right-hand side. This is obtained by properly mapping discrete test functions into $H^1_0(\varOmega )$.
Trends in Morbidity, Mortality, and Cost of Hospitalizations Associated With Infectious Disease Sequelae of the Opioid Epidemic
Authors: Coyle Joseph R , Freeland Melissa , Eckel Seth T , Hart Adam L 
Published: January 2020

Abstract

Background
Increases in fatal drug poisonings and hepatitis C infections associated with the opioid epidemic are relatively well defined, because passive surveillance systems for these conditions exist. Less described is the association between the opioid epidemic and skin, soft-tissue, and venous infections (SSTVIs), endocarditis, sepsis, and osteomyelitis.

Methods
Michigan hospitalizations between 2016 and 2018 that included an International Classification of Diseases, Tenth Revision, Clinical Modification, code indicating substance use were examined for codes indicative of infectious conditions associated with injecting drugs. Trends in these hospitalizations were examined, as were demographic characteristics, discharge disposition, payer, and cost data.

Results
Among hospitalized patients with a substance use diagnosis code, endocarditis, osteomyelitis, sepsis, and SSTVI hospitalizations increased by 33%, 35%, 24%, and 12%, respectively between 2016 and 2018. During this time frame, 1257 patients died or were discharged to hospice. All SSTVI hospitalizations resulted in &gt;$1.3 billion in healthcare costs. Public insurance accounted for more than two-thirds of all hospitalization costs.

Conclusions
This study describes a method for performing surveillance for infection-related sequelae of injection drug use. Endocarditis, osteomyelitis, sepsis, and SSTVI hospitalizations have increased year over year between 2016 and 2018. These hospitalizations result in significant morbidity, mortality, and healthcare costs and should be a focus of future surveillance and prevention efforts.
Sensory Nociceptive Neurons Contribute to Host Protection During Enteric Infection With Citrobacter rodentium
Authors: Ramirez Valerie T , Sladek Jessica , Godinez Dayn Romero , Rude Kavi M , Chicco Pamela , Murray Kaitlin , Brust-Mascher Ingrid , Gareau Melanie G , Reardon Colin 
Published: January 2020

AbstractBackgroundNeurons are an integral component of the immune system that functions to coordinate responses to bacterial pathogens. Sensory nociceptive neurons that can detect bacterial pathogens are found throughout the body with dense innervation of the intestinal tract.MethodsIn this study, we assessed the role of these nerves in the coordination of host defenses to Citrobacter rodentium. Selective ablation of nociceptive neurons significantly increased bacterial burden 10 days postinfection and delayed pathogen clearance.ResultsBecause the sensory neuropeptide CGRP (calcitonin gene-related peptide) regulates host responses during infection of the skin, lung, and small intestine, we assessed the role of CGRP receptor signaling during C rodentium infection. Although CGRP receptor blockade reduced certain proinflammatory gene expression, bacterial burden and Il-22 expression was unaffected.ConclusionsOur data highlight that sensory nociceptive neurons exert a significant host protective role during C rodentium infection, independent of CGRP receptor signaling.
Uncovering novel susceptibility targets to enhance the efficacy of third-generation cephalosporins against ESBL-producing uropathogenic Escherichia coli
Authors: Phan Minh-Duy , Bottomley Amy L , Peters Kate M , Harry Elizabeth J , Schembri Mark A 
Published: January 2020

AbstractBackgroundUropathogenic Escherichia coli (UPEC) are a major cause of urinary tract infection (UTI), one of the most common infectious diseases in humans. UPEC are increasingly associated with resistance to multiple antibiotics. This includes resistance to third-generation cephalosporins, a common class of antibiotics frequently used to treat UTI.MethodsWe employed a high-throughput genome-wide screen using saturated transposon mutagenesis and transposon directed insertion-site sequencing (TraDIS) together with phenotypic resistance assessment to identify key genes required for survival of the MDR UPEC ST131 strain EC958 in the presence of the third-generation cephalosporin cefotaxime.ResultsWe showed that blaCMY-23 is the major ESBL gene in EC958 responsible for mediating resistance to cefotaxime. Our screen also revealed that mutation of genes involved in cell division and the twin-arginine translocation pathway sensitized EC958 to cefotaxime. The role of these cell-division and protein-secretion genes in cefotaxime resistance was confirmed through the construction of mutants and phenotypic testing. Mutation of these genes also sensitized EC958 to other cephalosporins.ConclusionsThis work provides an exemplar for the application of TraDIS to define molecular mechanisms of resistance to antibiotics. The identification of mutants that sensitize UPEC to cefotaxime, despite the presence of a cephalosporinase, provides a framework for the development of new approaches to treat infections caused by MDR pathogens.
Effects of different carbohydrate sources on taurine status in healthy Beagle dogs
Authors: Pezzali Julia Guazzelli , Acuff Heather L , Henry Will , Alexander Celeste , Swanson Kelly S , Aldrich Charles G 
Published: January 2020

Abstract
This study evaluated the effects of a grain-based (GB) and grain-free (GF) diet on protein utilization and taurine status in healthy Beagle dogs. Two practical dog diets sufficient in crude protein, sulfur amino acids, and taurine content were formulated with the same ingredients with exception of the carbohydrate sources. The GB contained sorghum, millet, and spelt while potatoes, peas, and tapioca starch were used in the GF. A total of 12 Beagle dogs were used in a completely randomized design with six replicates per treatment. The study consisted of an adaptation period of 2 wk followed by an experimental period of 28 d in which GB and GF were fed to the dogs. At the end of the adaptation period and every 2 wk after it (day 0, day 14, day 28), markers of taurine metabolism were analyzed in whole blood (taurine), plasma (taurine, methionine, and cystine), urine (taurine:creatinine), and fresh fecal samples (primary and secondary bile acids). Fecal samples were collected during the last 6 d of experimental period for digestibly assessment using titanium dioxide as an external marker. Taurine markers and digestibility data were analyzed in a repeated measures model and one-way ANOVA, respectively, using PROC GLIMMIX in SAS (version 9.4). Apparent crude protein digestibility was not affected by treatment, but dogs fed GF diet had lower apparent organic matter digestibility compared with those fed GB (P &lt; 0.05). Greater plasma taurine concentrations were observed at days 14 and 28 compared with day 0; wherein dogs fed GF exhibited greater increase compared to those fed GB (P &lt; 0.05). Whole blood taurine concentrations, plasma methionine concentrations, and urinary taurine:creatinine were also greater at days 14 and 28 compared with day 0 (P &lt; 0.05), but no effect of diet was observed. Total bile acid excretion was similar between GF and GB groups, but dogs fed GF excreted a higher proportion of primary bile acids compared with those fed GB (25.49% vs. 12.09% at day 28, respectively). In summary, overall taurine status was not affected by dietary treatments, however, our results suggest that the higher content of oligosaccharides and soluble fibers in the GF diet may alter the composition of the fecal bile acid pool.
Decreased ADAM17 expression in the lungs of α-Klotho reduced mouse
Authors: Akasaka-Manya Keiko , Manya Hiroshi , Nadanaka Satomi , Kitagawa Hiroshi , Kondo Yoshitaka , Ishigami Akihito , Endo Tamao 
Published: January 2020

AbstractThe deficiency of α-Klotho in mice causes phenotypes resembling human age-associated disorders at 3–4 weeks after birth and shows short lifespans of ∼2 months. One of the crucial symptoms is pulmonary emphysema, although α-Klotho is not expressed in the lungs. α-Klotho secreted from the kidneys is probably involved in the pathology of emphysema because kidney-specific knockout mice exhibit emphysematous structural changes. We examined whether any glycan changes in α-Klotho mouse lungs were observed, because α-Klotho is reported to have glycosidase activity. Here, we found the accumulation of heparan sulphate in the microsomal fraction of α-Klotho mouse lungs. Meanwhile, a disintegrin and metalloproteinase 17 (ADAM17) expression was decreased in α-Klotho mice. From these results, it is thought that the increase in heparan sulphate is due to insufficient cleavage of the core protein by ADAM17. Additionally, a reduction in α-Klotho and a decline of ADAM17 were also observed both in normal aged mice and in senescence marker protein-30 (SMP30) knockout mice, a mouse model of premature ageing. Thus, the decrease in ADAM17 is caused by the reduction in α-Klotho. These may be involved in the deterioration of lung function during ageing and may be associated with the pathology of pulmonary emphysema.
Vital, Porcine, Gal-Knockout Skin Transplants Provide Efficacious Temporary Closure of Full-Thickness Wounds: Good Laboratory Practice-Compliant Studies in Nonhuman Primates
Authors: Holzer Paul , Adkins Jon , Moulton Krysta , Zhu Laurence , Monroy Rod , Cetrulo Curtis L 
Published: January 2020

AbstractVital, genetically engineered porcine skin transplants have long been regarded as a promising treatment option for severe burn wounds. The objective of this two-part, preclinical study was to evaluate the ability of vital, split-thickness skin xenotransplants derived from designated pathogen-free, alpha 1,3 galactosyltransferase knockout miniature swine to provide temporary wound closure of full-thickness wound defects intended to model severe and extensive, deep partial- and full-thickness burn wounds. In part 1 of the study, four full-thickness wound defects were introduced in four cynomolgus macaques recipients and, then engrafted with two xenografts and two allografts to achieve temporary wound closure. On POD-15, autografts were used to achieve definitive wound closure and were observed until POD-22. In part 2 of the study, four additional subjects each received two full-thickness wound defects, followed by two xenografts to achieve temporary wound closure, and were observed postoperatively for 30 days without further intervention. All grafts were assessed for signs of adherence to the wound bed, vascularity, and signs of immune rejection via gross clinical and histological methods. Xenograft and allograft comparators were equivalent in part 1, and later autografts were otherwise indistinguishable. In part 2, all xenotransplants demonstrated adherence, vascularity, and survival until POD-30. These were unexpected results that exceed previously published findings in similar models. Furthermore, the ensuing GLP-study report directly supported regulatory clearance, permitting a phase I clinical trial. This solution holds great promise as an alternative to human cadaver allograft, the current standard of care for the treatment of severe burns.