bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genome-wide Identification of the POD Gene Family and their Expression Profiling in Grapevine (Vitis vinifera L)
Authors: Xiao Huilin , Wang Chaoping , Khan Nadeem , Chen Mengxia , Fu Weihong , Guan Le , Leng Xiangpeng 
Published: October 2019

Abstract
The purpose of this study is by extending the analysis of class III peroxidases (PODs) in grapevine and provide further insights into the organ-specific developmental role in transcriptional dynamics and gene duplication analysis of this economically important fruit crop species. Herein, we comprehensively identified 47 PODs in the grapevine genome and are further classified into 7 subgroups based on their phylogenetic analysis. Results of motif composition and gene structure organization analysis revealed that PODs in the same subgroup shared similar conjunction while the protein sequences were highly conserved. Intriguingly, the integrated analysis of chromosomal mapping and gene collinearity analysis proposed that both dispersed and tandem duplication events contributed to the expansion of PODs in grapevine. Also, the gene duplication analysis suggested that most of the genes (20) were dispersed followed by (15) tandem, (9) segmental or whole-genome duplication, and (3) proximal, respectively. The evolutionary analysis of PODs, such as Ka/Ks ratio of the 15 duplicated gene pairs were less than 1.00, indicated that most of the gene pairs exhibiting purifying selection and 7 pairs underwent positive selection with value greater than 1.00. The Gene Ontology Enrichment (GO), Kyoto Encyclopedia of Genes Genomics (KEGG) analysis, and cis-elements prediction also revealed the positive functions of PODs in plant growth and developmental activities, and response to stress stimuli. Further, based on the publically available RNA-sequence data, the expression patterns of PODs in tissue-specific response during several developmental stages revealed diverged expression patterns. Subsequently, 30 genes were selected for RT-PCR validation in response to (NaCl, drought, and ABA), which showed their critical role in grapevine. In conclusion, we predict that these results will lead to novel insights regarding genetic improvement of grapevine.
Interference of functional dual-tasks on gait in untrained people with Parkinson’s disease and healthy controls: cross-sectional study
Authors: Valenzuela Constanza San Martín , Dueñas-Moscardó Lirios , López-Pascual Juan , Serra-Añó Pilar , Miguel José Manuel Tomás 
Published: October 2019

Abstract
Background: In Parkinson’s disease (PD) population, performing secondary tasks while walking further deteriorates gait and restrict mobility in functional contexts of daily life. This study (1) analyzed the interference of functional cognitive and motor secondary task on untrained people with PD and (2) compared their walking with healthy subjects. Methods : Forty people with PD (aged 66.72 [7.5] years, Hoehn and Yahr stage I-II-III on-medication) composed the PD group (PDG) and 43 participants (aged 66.60 [8.75] years) formed the group of healthy counterparts (HG). Gait was evaluated through spatiotemporal, kinematic and kinetic outcomes in five conditions: single task (ST) and visual, verbal, auditory and motor dual-task (DT). Results : The performance of both groups was higher in the ST than verbal, auditory and motor DT conditions (p < .05). A similar gait pattern was observed between ST and visual DT during velocity, cadence and vertical forces in PDG and HG. In addition, PDG exhibited a better double support time, ankle range, midstance and braking forces in the visual DT than the verbal and motor DT (p < .05). PDG participants had a significantly lower performance than the HG in all the variables analyzed except for the maximum hip extension in the stance phase (p>0.05). Conclusions : In untrained participants with PD, verbal and motor secondary tasks affect gait significantly, while auditory and visual tasks interfere to a lesser extent. Untrained patients with PD have a poorer gait performance than their healthy counterparts, but in different grades according to the analyzed variables. Trial registration : The data in this paper are part of a single-blind, randomized, controlled trial and correspond to the evaluations performed before a physical rehabilitation program, retrospectively registered with number at clinicaltrial.gov NCT04038866 .
Does long-term recreational gymnastics prevent injurious falls in older women? A prospective 20-year follow-up
Authors: Uusi-Rasi Kirsti , Karinkanta Saija , Kannus Pekka , Tokola Kari , Sievänen Harri 
Published: October 2019

Abstract
Background : Exercise interventions focused on balance and strength training have been shown to be effective for falls prevention. The aim of this 20-year register-based follow-up was to examine whether long-term participation in recreational female gymnastics is associated with a lower risk of medically-attended injurious falls.Methods : Health care register data of 187 women (103 recreational gymnasts and 84 sedentary controls) from the original cohort of 243 women were assessed. The mean age (sd) at baseline was 62.8 (5.4) years and the mean follow-up time was 19.4 (2.7) years (range from 5.6 to 21.0 years). Injurious falls were scrutinized from medical records. An injurious fall was defined as an event in which falling was mentioned as a reason for making contact with the health-care professionals. Negative binomial regression was used to estimate incidence rate ratios (IRR) for injurious falls, and Cox-regression models for calculating hazard ratios (HR) for injured fallers with the control group as reference.Results Recreational gymnasts had about 30% less injurious falls compared to controls, the mean IRR (95% CI) being 0.71 (0.51 to 0.96). Regarding injured fallers, the HR was 0.73 (0.52 to 1.02) favoring the recreational gymnasts. There were no statistically significant between-group differences for fractures.Conclusions: Long-term recreational gymnastics appears to reduce the risk of injurious falls in old age.
The Impact of Frailty On Admission To Home Care Services And Nursing Homes: Eight-Year Follow-Up Of A Community-Dwelling, Elderly, Spanish Cohort
Authors: Cegri Francisco , Orfila Francesc , Abellana Rosa M , Pastor-Valero María 
Published: October 2019

Abstract
Background The aim of this study is to identify the factors that anticipate the future inclusion of community-dwelling individuals aged ≥ 70 years in home care programmes (HC) or nursing homes (NH) and to develop the corresponding prediction models. Methods Study design: Prospective, multicentre, cohort study in 23 primary health care centres located in Catalonia, Spain, with an eight-year follow-up (2005-2013). Participants: the cohort was made up of 616 individuals. Data collection: Baseline interview included a multidimensional assessment carried out by primary health care professionals. Outcome variables were collected during follow-up by consulting electronic health care records, telephone contacts, and the Central Registry of Catalonia for mortality. Statistical analysis: A prognostic index for HC and NH at eight years was estimated for each patient. Death prior to these events was considered as a competing risk event, and Fine–Gray regression models were used. The internal validity of the predictive models was tested for 150 bootstrap re-samples.Results At baseline, mean age was 76.4 years, 55.5% were women, and 22% lived alone. During follow-up, 19.2% entered an HC program, 8.2% a NH, and 15.4% died without presenting an event. Of those who entered a NH, 31.5% had previously been in an HC program. Multivariate competitive risk models for HC and NH showed that the risk of HC entry was associated with older age, dependence on the Instrumental Activities of the Daily Living, and slow gait measured by Timed-up-and-go test. An increased risk of being admitted to a NH was associated with older age, dependence on the Instrumental Activities of the Daily Living, augmented number of prescriptions, and the presence of social risk. Conclusions Prognostic models based on comprehensive geriatric assessments can predict the need for the commencement of HC and NH admission in community-dwelling older adults. Our findings underline the necessity to measure functional capacity, mobility, number of prescriptions, and social aspects of the elderly in the primary healthcare centres. In such a setting they can be offered longitudinal holistic assessments so as to benefit from preventive actions in order to remain independent in the community for as long as possible.
Relationships between cystatin C- and creatinine-based eGFR in Japanese rural community-dwelling older adults with sarcopenia
Authors: Kusunoki Hiroshi , Tsuji Shotaro , Kusukawa Tomoyuki , Wada Yosuke , Tamaki Kayoko , Ohta Yoshinori , Nagai Koutatsu , Itoh Masako , Sano Kyoko , Amano Manabu , Maeda Hatsuo , Sugita Hideyuki , Hasegawa Yoko , Kishimoto Hiromitsu , Shimomura Soji , Shinmura Ken 
Published: October 2019

Abstract
Background Sarcopenia is prevalent in patients with chronic kidney disease (CKD). Sarcopenia is prevalent in patients with chronic kidney disease (CKD). The indices of physical function, such as grip power and gait speed, decreased according to the decline in estimated glomerular filtration rate (eGFR). Methods We examined the relationships between cystatin C-based GFR (eGFRcys), and creatinine-based GFR (eGFRcre), and their ratio (eGFRcys/eGFRcre) and low skeletal muscle mass index (SMI) in community-dwelling older adults in Japan. This cross-sectional study included 286 men aged 73.3±6.2 years and 606 women aged 72.9±5.8 years from a rural area in Hyogo Prefecture, Japan. eGFRcys and eGFRcre were simultaneously measured, whereas low SMI based on the AWGS criteria was evaluated. Results eGFRcys and the eGFRcys/eGFRcre were significantly correlated with grip power and gait speed. The eGFRcys/eGFRcre was also correlated with SMI. In the multivariate logistic regression analysis, when the eGFRcys/eGFRcre was added as a covariate to the basic model, it was significantly associated with low SMI, both in all subjects. Moreover, CKDcys with a low eGFRcys/eGFRcre ratio (<1.0) was associated with a higher risk of low SMI than CKDcys alone. Conclusion In conclusion, CKDcys but not CKDcre is an independent risk factor of low SMI. In patients with CKDcys, lower eGFRcys/eGFRcre may be a practical screening marker of low SMI in community-dwelling older adults.
Mediating effect of perceived stress between social capital and work satisfaction among modern service industry workers in Shanghai
Authors: Wu Ning , Li Zan , Dai Junming , Wang Chao , Gao Junling , Fu Hua 
Published: October 2019

Abstract
Background To explore the relationship among social capital, perceived stress, and work satisfaction to provide a basis for improving work satisfaction among modern service industry workers.Methods A total of 737 employees from two units belonging to modern service industries in Shanghai were investigated through purposive sampling. A self-administered anonymous questionnaire was used to collect data. The relationships among these variables were analyzed using a multiple linear regression model and structural equation model.Results After controlling for demographics, perceived stress was shown to be a negative predictor of work satisfaction, while social capital had a significant positive predictive effect. Structural equation modeling confirmed that perceived stress partially mediated the relation between social capital and work satisfaction.Conclusion Social capital could be an efficient method for improving work satisfaction directly or indirectly by reducing perceived stress.
Investigating Relationship Between Religious Commitment and Moral Sensitivity in Nurses Working in ICU
Authors: khalighi ebrahim , Solaimanizade Laleh , borji milad , tarjoman asma , soltani behrouz , zarei farzad 
Published: October 2019

Abstract
Objectives this study aimed at determining the relationship between religious commitment and moral sensitivity among nurses working in the ICU sections in the west of Iran. The present study was a cross-sectional descriptive-analytic study carried out on nurses working in ICU wards of two western cities in Iran. The instrument used includes a demographic questionnaire, religious commitment questionnaire and moral sensitivity questionnaire in nurses. First, the researchers referred to the ICU wards of the hospitals in the cities after receiving permission from the relevant authorities by referring to three shifts in the morning, evening and night shifts and holidays. The researchers, while explaining the research goals for the nurses participating in the study, obtained their informed consent to participate in this study. Results : According to the findings, mean (SD) of the overall score of religious commitment was equal to 36.38 (4.58) and mean (SD) of MS score of nurses was 59.21 (12.65). Also, 91 nurses (82.7%) had average MS, 7 (6.4%) had low MS and 12 (10.9%) had high MS. Keywords: Religious Commitment, Moral Sensitivity, Nurse
Informative Supraglottic Airway versus Endotracheal Tube During Interventional Pulmonary Procedures – A Retrospective Study
Authors: Behrens Kyle M , Galgon Richard E 
Published: October 2019

Abstract
Background: As the field of interventional pulmonology (IP) expands, anesthesia services are increasingly being utilized when complex procedures of longer duration are performed on sicker patients with high risk co-morbidities and lung pathology. Yet, evidence on the optimal anesthetic management for these patients remains lacking. Our aim was to characterize the airway management and, secondarily anesthetic maintenance patterns used for IP procedures at our institution. Methods: From 2894 identified encounters, charts of 783 patients undergoing an IP procedure with general anesthesia over a 5-year period, employing an endotracheal tube (ETT) or a supraglottic airway (SGA) for airway maintenance, were identified and reviewed after exclusions. Patients posted for a concurrent thoracic surgical procedure and those already intubated at presentation were excluded. Baseline patient demographics, procedure, proceduralist type, anesthesia maintenance modality, neuromuscular blocking drug (NMBD) use, and airway management characteristics were extracted and analyzed. Results: Inhaled general anesthesia with an ETT for airway maintenance was most commonly employed; however, SGAs were used in one-third of patients with a very low conversion rate (0.4%), and their use was associated with a significant reduction in NMBD use. Conclusions: In this large series of patients receiving general anesthesia for IP procedures, inhaled anesthetic agents and ETTs were favored. However, in appropriately selected patients, SGA use was effective for airway maintenance and allowed for a reduction in NMBD use, which may have implications in this patient population who may have an increased risk for pulmonary complications and warrants further investigation.
Nutrient Source and Tillage Effects on Maize: II. Yield, Soil Carbon, and Carbon Dioxide Emissions
Authors: O'Dell Deb , Eash Neal S. , Zahn James A. , Hicks Bruce B. , Oetting Joel N. , Sauer Thomas J. , Lambert Dayton M. , Logan Joanne , Goddard John J. 
Published: October 2019

Core Ideas
Industrial biotechnology waste as a soil amendment approximates farmer practice yields.
Applying waste amendments significantly increased soil organic C compared with farmer practice.
Using waste nutrients for agriculture and soil organic matter improves sustainability.
Reuse of industrial biotechnology by‐products has become an important component of circular bio‐economies whereby nutrient‐rich wastes are returned to agricultural land to improve soil fertility and crop productivity. Heat‐inactivated spent microbial biomass (SMB) from the production of 1,3‐propanediol is an industrial fermentation by‐product with nutrients that could replace or supplement conventional fertilizers. Our objectives were to determine if SMB utilization as a soil amendment in agriculture could generate environmental benefits while meeting farmer yield expectations and assess the impact of SMB application on CO2 emissions. This study examined the replacement of typical farmer fertilizer practices with the application of SMB. In addition to yellow dent maize (Zea mays L. var. indentata) grain yield and aboveground biomass, soil organic carbon (SOC) was measured. The eddy covariance (EC) micrometeorological method was used to measure CO2 flux. Overall maize yields were positively correlated with increasing application rates of SMB. After two SMB applications, SOC increased by 45% on the SMB plot as compared with an increase of 11% on the farmer practice plot. The SMB‐treated plot also emitted more CO2 (794 g CO2 m−2 yr−1) compared with the farmer practice treatment (274 g CO2 m−2 yr−1). Results from this study provide information on the efficacy of waste product nutrient cycling in the soil–plant ecosystem that could improve productivity and sustainability.
Soil Chemical Property Changes in Response to Long‐Term Pineapple Cultivation in Costa Rica
Authors: Sherman Leslie A. , Brye Kristofor R. 
Published: October 2019

Core Ideas
Soil organic C was lower under pineapple cultivation than in an adjacent pasture and secondary forest.
Soil organic C did not differ between 7‐ and 20‐yr‐old pineapple fields.
Soil pH and base cations were lower in a 20‐ than in a 7‐yr‐old pineapple field, pasture, and forest.
Anion exchange capacities were larger in soils under pineapple cultivation than in pasture soils.
Pineapple cultivation may require increased liming rates to help maintain long‐term soil quality.
Pineapple [Ananas comosus (L.) Merr.] has been cultivated in Costa Rica for decades on land previously under forest and pasture vegetation. A pineapple plantation in Costa Rica was chosen to investigate the impact of long‐term pineapple cultivation on soil chemical properties. Soil samples were collected at pineapple sites under cultivation for 7 and 20 yr, as well as in an adjacent pasture and secondary forest. Samples were analyzed for extractable nutrients, total C and N, and cation and anion exchange capacities (CEC and AEC, respectively). Soil organic carbon (SOC) and soil N concentrations did not differ (P > 0.05) between the 7‐ and 20‐yr‐old pineapple sites nor between the pasture and forest sites in the top 20 cm. However, the average SOC concentration in the pasture and forest sites was 1.5 times greater (P < 0.05) than that in the pineapple sites. Soil AEC was 3.5 times greater (P < 0.05) in the top 10 cm of both pineapple sites than in the pasture. The 20‐yr‐old pineapple site, which had a soil pH of 4.1, had 2 to 13 times greater (P < 0.05) exchangeable acidity and 2 to 5 times lower base cation concentrations in the top 20 cm than the 7‐yr‐old pineapple, pasture, and forest sites. The greater acidity and lower base cation concentrations in the older pineapple site suggest that agronomic management practices may need to be adjusted to minimize negative changes in soil chemical properties and help maintain future soil productivity.
Research Gaps and Challenges in the Conservation and Use of North American Wild Lettuce Germplasm
Authors: Lebeda Aleš , Křístková Eva , Kitner Miloslav , Majeský Ľuboš , Doležalová Ivana , Khoury Colin K. , Widrlechner Mark P. , Hu Jinguo , Carver Daniel , Achicanoy Harold A. , Sosa Chrystian C. 
Published: October 2019

ABSTRACTThe North American crop wild relatives (CWR) of lettuce (Lactuca L.) represent an underexplored pool of genetic diversity of potential value to breeding programs. The 10 species belong to three different groups: a native clade including at least six allotetraploid species [L. biennis (Moench) Fernald, L. canadensis L., L. floridana (L.) Gaertn., L. graminifolia Michx., L. hirsuta Muhl. ex Nutt., and L. ludoviciana (Nutt.) Riddell], a diploid clade with one species [L. tatarica (L.) C. A. Mey. subsp. pulchella (Pursh) Stebbins], and a clade related to the cultivated taxon (L. sativa L.) with three non‐native species (L. saligna L., L. serriola L., and L. virosa L.). In this review, we examine the role of herbarium and genebank holdings in taxonomic and other foundational studies, as well as for germplasm exploration and use. We compile the state of knowledge on the ranges of lettuce CWR in North America, modeling the potential distributions of the species and assessing their ex situ and (for native species) in situ conservation status. We categorize seven of the species as high priority for further conservation and three as medium priority, with none currently considered low priority or sufficiently conserved. Further, we review morphological, phenological, genetic diversity, and pest and disease information with regard to North American species. We conclude by outlining the critical gaps and describing a way forward for addressing challenges in the conservation and use of North American wild lettuce germplasm.
Elucidating the Role of Val-Asn 95 and Arg-Gly 52 Mutations on Structure and Stability of Fibroblast Growth Factor Homologous Factor 2
Authors: Kolli Vidyalatha , Paul Subhankar , Guttula Praveen Kumar , Sarkar Nandini 
Published: October 2019

Background:Fibroblast growth Factor Homologous Factors (FHFs) belong to a subclass of Fibroblast Growth Factor (FGF) family owing to their high sequence and structural similarities with FGFs. However, despite these similarities, there are properties which set them apart from FGFs. FHFs lack the secretion signal sequence unlike other FGF members, except FGF1 and 2. Unlike FGFs, FHFs are not able to bind to FGF Receptors (FGFRs) and instead have been implicated in binding to Voltage-Gated Sodium Channels (VGSCs), neuronal MAP kinase scaffold protein and islet-brain-2 (IB2). The two amino acids Arg-52 and Val95 are conserved in all FHFs and mutation of these residues lead to its inability to bind with VGSC/IB2. However, it is not clear whether the loss of binding is due to destabilization of the protein on mutation or due to involvement of Arg52 and Val95 in conferring functionality to FHFs.Objective:In the present study, we have mutated these two conserved residues of FHF2 with its corresponding FGF counterpart amino acids and studied the effects of the mutations on the structure and stability of the protein.Methods:Several biophysical methods like isothermal equilibrium denaturation study, ANS fluorescence, intrinsic fluorescence, acrylamide quenching, circular dichroism studies as well as using computational approaches were employed.Results:The single mutations were found to affect the overall stability, conformation and functionality of the protein.Conclusion:Thus, the studies throw light on the role of specific amino acids in deciding the stability, structure and functionality of proteins and will be useful for development of therapeutically engineered proteins.