bluuebunny/crossref_metadata_2025_split huggingface dataset:
MiR-142-3p could serve as a potential biomarker for individualized treatment of solitary and multiple leiomyomas
Authors: Xue Luqi , Yang E , Li Zhengyu , Gou Jinhai , Nie Dan , Jia Jia , Feng Juntao , Yi Tao 
Published: February 2020

Abstract
Background: The pathogenesis and clinical behaviors between solitary leiomyoma (SL) and multiple leiomyomas (ML) vary, which lead to the difference in management for childbearing-aged patients. Herein, we aim to find the potential miRNA biomarkers for optimizing the individualized management between SL and ML.Methods: A microarray analysis was conducted to screen out the potentially dysregulated miRNAs. Target genes and signaling pathway potentially involved in UL pathogenesis were predicted by bioinformatics. The effect of miRNA was examined by Cell Counting Kit-8 proliferation assay and qRT-PCR after transfection of miRNA mimicsResults: The top 5 differentially expressed miRNAs, Wnt signalling pathway and its two central molecules APC and CTNNB1 were screened out according to microarray analysis and bioinformatics. MiR-142-3p was selected for further exploration. In validation of qRT-PCR, MiR-142-3p was significantly upregulated in SL, while downregulated in ML, CTNNB1 and sequencing target AXIN-2 were expressed at higher level in ML than SL. Overexpression of MiR-142-3p resulted in lower transcription level of CTNNB1 and AXIN-2, and lower cell proliferation level.Conclusions: MiR-142-3p may be involved in the development of SL and ML by interacting with CTNNB1 and AXIN-2 through Wnt signaling pathway. MiR-142-3p could serve as a potential biomarker for individualized treatment between SL and ML in the future.
Arthroscopic ankle fusion only has a limited advantage over the open operation if osseous operation type is the same: a retrospective comparative study
Authors: Wang Chenggong , Xu Can , Li Mingqing , Li Hui , Wang Long , Zhong Da , Liu Hua 
Published: February 2020

Abstract
Background: A great deal of research suggests that arthroscopic ankle fusion (AAF) has advantages over open ankle fusion (OAF), but these outcomes would be imprecise because of a selection bias. The purpose of this study is to verify which is better for ankle fusion, AAF or OAF. We regrouped the OAF group into two subgroups according to whether the osseous operation type is the same as AAF group. The goal is to minimize the impact of disease severity, thereby reducing selection bias to some extent.Methods: We retrospectively analyzed the data of ankle fusion in our hospital between July 2015 and October 2018. 43 patients were enrolled and divided into AAF group (n=17) and OAF group (n=26). In order to eliminate selection bias, we divided OAF group into complex osseous operation subgroup (COO subgroup) (n=15) and simple osseous operation subgroup (SOO subgroup) (n=11). The osseous operation type of SOO subgroup is the same as AAF group. Then, we compared the differences between these groups. All patients were followed up at least one year after operation. We analyzed data, including etiology composition, surgical time, intra-op blood loss, reduction of albumin, total hospital stays, union time, fusion situation, complications, radiological examination, functional score and questionnaire survey. Then we performed statistical analyses.Results: We found that the etiological components of AAF group and OAF group were different; the etiological components of AAF group and SOO subgroup were similar. We found that AAF group has advantages over OAF group and COO subgroup in general. However, except in terms of surgical trauma, hospital stays and short-term complications occurred, the AAF group has not obvious advantages over SOO subgroup, including intra-op blood loss, fusion condition, postoperative function score and postoperative patient satisfaction; and AAF group need more surgical time than the SOO subgroup.Conclusions: The arthroscopic ankle fusion can bring a good curative effect; however, if the osseous operation type is the same, the arthroscopic ankle fusion only has a limited advantage over the traditional open operation in perioperative soft tissue protection and enhanced recovery after surgery.
Differential expression and significance of Stat3 and IL-17 in gastritis and gastric intraepithelial neoplasia and gastric cancer
Authors: Wang Lijie , Cao Chong , Wang Xiao Jun , Tao Lin , Li Feng , Zhang Wen Jie 
Published: February 2020

Abstract
Background: The role of Stat3 and IL-17 in the development of gastric cancer was investigated by detecting Stat3 activation level and IL-17 expression in gastritis, GIN and gastric cancer.Methods: (1) We collected 70,137 patients who underwent gastroscopy at the First Affiliated Hospital of Shihezi University Medical School from November 2007 to December 2015, coded and screened all the diagnostic results. (2) We used immunohistochemical to detect differences in phosphorylated Stat3 and IL-17 expression in gastritis, GIN, and gastric cancer. (3) Kruskal-Wallis H test compared IL-17 or p-Stat 3 expression in gastric cancer, GIN and gastritis; Non-parametric test compared difference of IL-17 expression and p-Stat3 activation level between randomized and disease progression groups indifferent gastric diseases.Results: (1)The results of immunohistochemistry showed that as the degree of disease increased, the staining increased too; (2) There were differences in Stat3 activation level and IL-17 expression between gastritis, GIN and gastric cancer tissues (P<0.001). With the disease increasingly serious, the Stat3 activation level and IL-17 expression are higher; (3) The ROC of p-Stat3 is the largest (0.923, P < 0.001) and the ROC of IL-17 is 0.844 (P < 0.001); (4) The activated Stat3 and IL-17 both are the risk factors of gastric cancer.Conclusions: (1) With the disease increasingly serious, the Stat3 activation level and IL-17 expression are higher. (2) There is a high correlation between activated Stat3 and IL-17 expression, both of which are risk factors for gastric cancer.
MicroRNA 29c-3p indicates advanced liver fibrosis/cirrhosis in univentricular heart patients with and without Fontan palliation
Authors: Abu-Halima Masood , Meese Eckart , Saleh Mohamad Ali , Keller Andreas , Abdul-Khaliq Hashim , Raedle-Hurst Tanja 
Published: February 2020

Abstract
Background: In patients with chronic liver disease that usually results in liver fibrosis, microRNAs (miRNAs) have been shown to be of diagnostic and prognostic value. The present study aims to identify those miRNAs in patients with univentricular heart (UVH) disease with and without Fontan palliation that may be associated with advanced liver fibrosis/cirrhosis.Methods: A large panel of human miRNA arrays were used to determine miRNA abundance profiles in the blood of 48 UVH patients with and without Fontan palliation and 32 age and gender-matched healthy controls. The most abundantly expressed miRNAs identified by microarray analysis were correlated to prognostic scores of advanced liver fibrosis/cirrhosis and selected miRNAs validated by RT-qPCR.Results: According to microarray analysis, 50 miRNAs were found to be significantly abundant in UVH patients of which miR-29b-3p and miR-29c-3p were significantly correlated to the model of end-stage liver disease (MELD)-Albumin and albumin-bilirubin (ALBI) score. According to ROC analysis, a MELD-Albumin score ≥ 11 was best predicted by total bilirubin (AUC 0.985, P=0.005), collapsibility index (AUC 0.952, P=0.009) and miR-29c-3p (AUC 0.941, P=0.011) and the most significant predictors of an ALBI score >−2.6 were collapsibility index (AUC 0.998, P<0.001) and albumin (AUC 0.981, P=0.006). In the multivariate analysis, miR-29c-3p turned out to be an independent predictor of advanced liver fibrosis/cirrhosis as defined by a MELD-Albumin score ≥ 11 (P=0.004) and ALBI score > −2.6 (P=0.009), respectively.Conclusion: In UVH patients with and without Fontan palliation, miR-29c-3p seems to be an independent predictor of advanced liver fibrosis/cirrhosis and thus may be used in the risk assessment of these patients.
Antibody responses to porcine reproductive and respiratory syndrome virus, influenza A virus, and Mycoplasma hyopneumoniae from weaning to the end of the finisher stage in fourteen groups of pigs in Ontario
Authors: Raaphorst Elana , Farzan Abdolvahab , Friendship Robert M. , Lillie Brandon N. 
Published: February 2020

Abstract
Background Porcine reproductive and respiratory syndrome, swine influenza, and mycoplasmal pneumonia are some of the most prevalent respiratory diseases affecting swine farm productivity in Canada. Monitoring for the prevalence of the infectious agents associated with these diseases on farm may help to improve herd-specific control strategies and to minimize the impact of disease on commercial swine farms. The objectives of this study were to investigate antibody responses to porcine reproductive and respiratory syndrome virus (PRRSV), influenza A virus (IAV), and Mycoplasma hyopneumoniae ( M. hyopneumoniae ) from weaning to the end of the finisher stage on a subset of commercial swine farms in Ontario and to examine the effects of nursery diet on antibody responses. Results Serology found 8, 61, and 31% of pigs at weaning, 1, 31, and 22% at the end of nursery, 8, 38, and 18% at the end of grower, and 11, 48, and 25% at the end of the finisher stage tested seropositive for PRRSV, IAV, and M. hyopneumoniae, respectively. Of the groups tested for PRRSV, IAV, and M. hyopneumoniae, 3, 14, and 5 groups had > 20% of pigs that tested seropositive at least once over the course of production (“high seropositivity”). In general, seropositivity was more likely to be lower at the end of nursery compared to the other production stages for all three pathogens, and more likely to be higher for PRRSV and IAV at weaning, end of grower, and end of finisher. Pigs that were seropositive for PRRSV were more likely to be seropositive forM. hyopneumoniae (p < 0.001). Overall, pigs fed a low complexity diet during nursery were more likely to be seropositive for PRRSV (p < 0.001) and IAV (p = 0.04). Conclusions This study provides information regarding changes in serum antibody in pigs across different stages of production and highlights periods of vulnerability. Additionally, these findings may encourage further research into the effects of nursery diet complexity on disease susceptibility and immune response.
A new perspective on the rise of tuberculosis cases: Communal living
Authors: Kurtuluş Şerif , Can Remziye , Sak Zafer Hasan Ali 
Published: February 2020

Abstract
Background: Forced migration caused by wars causes health parameters to regress including tuberculosis. Wars are obstacles that stand in our way to eradicate tuberculosis and they are the reason why it has been such a threat to human life. To determine the relationship between living area and family type and the number of contacts among refugees and Turkish citizens who have been diagnosed with tuberculosis.Method: This research has been conducted with 194 patients with tuberculosis who admitted to The Faculty of Medicine, Harran University between the years of 2012-2019. The number of contacted people has been provided with the patients' folders. Reaching their homes, the patients' family types and living areas have been recorded among the volunteer attenders.Result: 98 (%50,5) percent of the members of the study group are refugees, the number of the contacts, among screened refugees, is 549 while the size of the living area is 7740m2. 96 (%49,5) percent of the members of the study group are citizens of the Turkish Republic(TR), the number of the contacs, among screened TR citizens, is 487, while the size of the living space is 11370 m2. 57 percent (%58,2) of the refugee families were found in communal living style while 47 (%49,0) percent of TR citizens were found living in a nuclear family system. It has been found that the statistical significance between the living area and the family type is caused by the difference between the nuclear family system and the communal living style. The average living space of TR citizens is meaningfully higher than the average living area of the refugees.Conclusion: It has been found that tuberculosis infects more people in war-related life conditions and this is caused by people -who are not blood-related- living together, communal living in other words. This is new information for the literature. The exposed number being higher in refugees than TR citizens proves that the refugees living out of camp are present in communal living spaces more often. The obstacles to reaching health services for refugees living out of camp should be examined.
Fertility desire and associated factors among ART user reproductive-age women in public health facility in Gondar city administration Northwest Ethiopia
Authors: Gebrekidan Eyob Araya , Geremew Alehegn Bishaw , Bisetegn Telake Azale 
Published: February 2020

Abstract
Background: Antiretroviral therapy scales up globally and in Ethiopia, as a result, AIDS-related mortality has reduced, but the number of new HIV infections increasing. Antiretroviral therapy in Ethiopia gives a chance of living longer for reproductive-age women. Prevention of pregnancy among HIV positive women is the second WHO prongs of HIV transmission with respecting women’s reproductive rights. Antiretroviral therapy uses improve the health of HIV positive women using and women's fertility desire increasing. Therefore this study aimed to assess the fertility desire and associated factors among ART user reproductive-age women. Methods: A facility-based cross-sectional study design conducted from November 2-30/2018. The calculated sample size was 400 and study participants were selected by using systematic random sampling. A pre-tested structured questionnaire was used to collect data. A binary logistic regression model was employed and adjusted odds ratio with a 95% confidence interval used to consider statistically significant Results: A total of 397 clients were participated in the study, giving a response rate of 99.3%. The proportion of fertility desire in this study was 55.2 %(95% CI, 50.4%- 60.2%). A duration of marriage < 4 year [AOR=6.9(95 CI: 1.65, 28.81)], and 5–9 years duration of marriage [AOR= 13.8 (95% CI: 2.39, 80.39)] higher as compared to 15 years and above, family influence 3.4 times more likely to have fertility desire than have no family influence [AOR= 3.4 (95% CI: 1.06, 11.25)], partner desire 4.3 times more likely have had fertility desires as compared to with counterpart [AOR= 4.3 (95% CI: 1.93,9.41)], and discussion with health care provider 66 % less likely to have fertility desire as compared with counterpart [AOR = 0.34 (95% CI: 0.39, 3.31)]. Conclusion: In this study, we have identified a high proportion of fertility desire among ART user reproductive-age women. The duration of marital status, partner influence, family influence, and discussion of health care were associated with fertility desire. Family-oriented counseling, partner involvement on reproductive health services should be encouraged to support the rights of all women to safely achieve their fertility. Keywords: Fertility desire, reproductive age, women on ART.
Consumption of aquatic products and meats in Chinese residents: A cross-sectional study in 2014
Authors: Jiang Liying , Shen Junjie , Li Jianwen , Wang Huijun , Liu Sana , Su Chang , Liu Yujie , Zhuang Xun , Liu Aidong 
Published: February 2020

Abstract
Objective To characterize the distribution, potential determinants of the consumption of aquatic products and meats, as well as part of the nutritional behavior of Chinese residents in a nationwide representative sample.Methods The study was conducted using a multi-stage stratified random cluster sampling technique and a population-proportional sampling procedure in 14 provinces in China. Chinese subjects aged 3 years and above (n = 24,106) completed a face-to-face dietary interview from July to October in 2014.Results The average daily consumption of meat and aquatic products for the all-aged population was 70.9 g and 48.0 g, respectively, which aligned with Dietary Guidelines (40-75g/d) for Chinese Residents (2016). Intake of aquatic products among Chinese people was relatively insufficient, especially for adolescents and elder people (<40g/d). Males, mainly aged 19-60, generally consumed too much meat (>80g/d), and 19-44 grouping consumed more than 70g/d of red meat. Besides, urban residents and individuals with higher SES have exhibited more comprehensive dietary preferences than rural ones and those with a lower SES do. Women and the higher SES group tend to be closer to the dietary guidelines for the Chinese.Conclusions The consumption of meat and aquatic products varied with age, sex, region and SES. Detecting patterns in consumption is particularly relevant for policy makers, researchers and health professionals in the formulation of dietary recommendations and estimating potential health outcomes.
Oral treatment with iododiflunisal delays beta amyloid plaque formation in a transgenic mouse model of Alzheimer Disease: a Longitudinal in vivo molecular imaging study
Authors: Rejc Luka , Gómez-Vallejo Vanessa , Rios Xabier , Cossio Unai , Baz Zuriñe , Mujica Edurne , Giao Tiago , Cotrina Ellen Y , Jiménez-Barbero Jesús , Quintana Jordi , Arsequell Gemma , Cardoso Isabel , Llop Jordi 
Published: February 2020

Abstract
Background: Transthyretin (TTR) is a tetrameric, Amyloid-beta (Aβ)-binding protein, which has been shown to reduce Aβ toxicity both in vitro and in vivo. The ability of TTR to interact with Aβ can be enhanced by a series of small molecules that stabilize its tetrameric form. Because of this, TTR stabilizers might act as disease modifying drugs in Alzheimer Disease (AD). In this work, we monitored the therapeutic efficacy of two TTR stabilizers, iododiflunisal (IDIF) and the repurposed drug tolcapone, by longitudinal assessment of Aβ deposition in an animal model of AD using positron emission tomography (PET) with [18F]florbetaben. Methods: Mice (AβPPswe/PS1A246E/TTR+/-; n=21) were divided into 3 groups (n=7 per group): iododiflunisal (IDIF)-treated, tolcapone-treated and non-treated. The treatment, administered in the drinking water at a dose of 100 mg/Kg/day, was started at 5 months of age. The level of Aβ deposition was assessed at ages=5, 9, 11, and 14 months by PET imaging using [18F]florbetaben. Treatment efficacy was determined based on radiotracer uptake in the hippocampus (HIP) and the cortex (CTX) with respect to the cerebellum (CB) and presented as standardized uptake value ratios (SUVr). Immunohistochemical (IHC) analysis was performed at 14 months of age to further support in vivo results. Results: SUVr of [18F]florbetaben in CTX and HIP of non-treated animals progressively increased from age=5 to 11 months and stabilized afterwards. In contrast, [18F]florbetaben uptake in HIP of IDIF-treated animals remained constant between ages=5 and 11 months and significantly increased at 14 months. At age=11 months, IDIF-treated group showed significantly lower SUVr values than those obtained for non-treated animals. In tolcapone-treated group, SUVr progressively increased with time, but at lower rate than in non-treated group. Moderate treatment effect of tolcapone suggests different mechanism of action than IDIF. No significant treatment effect was observed in CTX of IDIF- or tolcapone-treated animals. Results from IHC matched the in vivo data at age=14 months. Conclusions: The TTR stabilizer IDIF shows good Aβ-protective effect. Nevertheless, Aβ levels in treated and control animals reached similar values at the end of the study. Furthermore, differences in efficacy between IDIF and tolcapone, suggest different mechanisms of action.
Progressive memory circuit impairments along with Alzheimer’s disease neuropathology spread: evidence from in vivo neuroimaging
Authors: Li Kaicheng , Wang Shuyue , Luo Xiao , Zeng Qingze , Jiaerken Yerfan , Xu Xiaopei , Wang Chao , Liu Xiaocao , Li Zheyu , Zhao Shuai , Zhang Tianyi , Fu Yanv , Chen Yanxing , Liu Zhirong , Zhou Jiong , Huang Peiyu , Zhang Minming 
Published: February 2020

Abstract
Background Along with Alzheimer’s disease (AD) continuum, AD neuropathologies propagate trans-neuronally, causing the memory circuit disorganization and memory deficit. However, no evidence supports the hypothesis in vivo to date. Methods Based on biological diagnosis criteria, we divided subjects into 5 groups by setting the CSF cutoff point at 192 pg/ml for Aβ 1-42 (A) and 23 pg/ml for P-tau 181 (T): Group 0, cognitively normal (CN) with normal Aβ 1-42 and P-tau 181 (A−T−); Group 1, CN with A+T−; Group 2, CN with A+T+; Group 3, mild cognitive impairments (MCI) with A+T+; Group 4, AD with A+T+. We defined the memory circuit as the hippocampus (HP), cingulum-angular bundles (CAB), and precuneus cortex, respectively representing the starting point, core connecting fiber, and connected downstream cortex. Then we assessed the HP subfields volume, CAB diffusion metric (whole tract-level and waypoint-wise), and precuneus volume. Finally, we correlated neuroimaging measures with cognitive and neuropathological data. Results Along AD continuum, HP subfields volume initially increased and then decreased, starting from the early stage (CN with A+T-). CAB integrity loss on both whole tract-level and waypoint-wise in MCI and AD with A+T+ and progressed along AD continuum. Regarding precuneus, we only found the decreased volume in MCI and AD with A+T+, with CN stage spared. Further, memory circuit structure impairment correlated with more AD neuropathology and worse memory profile. Conclusion Our results support the tau propagation theory in the memory circuit, suggested that the memory circuit impairments starting from the HP, then propagating to the downstream projection tract and cortex.
Clinical characteristics and prognosis of 40 patients with periorbital metastasis of neuroblastoma
Authors: Qin Jing , Zhao Qian , Jin Mei , Xu Xiao , Yue Zhixia , Qin Maoquan , Fan Hongjun , Yu Tong , He Lejian , Qin Hong , Ma Xiaoli 
Published: February 2020

Abstract
Background This study was performed to summarize the clinical characteristics of patients with periorbital metastasis of neuroblastoma (NB) and analyze the short-term prognosis. Methods This study included 40 patients with periorbital metastasis of NB from March 2007 to March 2018. The patients’ clinical characteristics and short-term prognosis were retrospectively analyzed. Results The 40 patients comprised 25 boys and 15 girls with a median age at onset of 31 months (range, 8–132 months). The patients’ symptoms included a periorbital mass and eye bruising (n = 37, 92.5%), limb pain (n = 14, 35.0%), a head and neck mass (n = 8, 20.0%), fever (n = 8, 20.0%), an abdominal mass (n = 4, 10.0%), weakness (n = 3, 7.5%), abdominal pain (n = 2, 5.0%), anemia (n = 2, 5.0%), and others (n = 3, 7.5%). All 40 patients had stage IV, high-risk NB. Various auxiliary structures of the eye were involved: ocular muscles (n = 11, 27.5%), lacrimal glands (n = 4, 10.0%), optic nerve (n = 2, 5.0%), and eyelid (n = 1 2.5%). Fifteen patients (37.5%) had tumor invasion in a periorbital sinus cavity. All patients had bone marrow and bone metastasis. Of 38 patients with a known MYCN gene status, 17 patients (44.7%) had MYCN amplification. The median follow-up duration was 19 months (range, 4–144 months). The 5-year event-free survival rate was 6%, and the 5-year overall survival rate was 16%. Conclusions Patients with periorbital metastasis of NB often have multi-organ metastases, extremely high rates of MYCN amplification and bone marrow metastasis, and a poor short-term prognosis.