bluuebunny/crossref_metadata_2025_split huggingface dataset:
Efficacy and safety of Shengjiang Xiexin decoction in prophylaxis of chemotherapy-related diarrhea in small cell lung cancer patients: study protocol for a multicenter randomized controlled trial
Authors: Deng Chao , Lou Yanni , Gao Yu , Deng Bo , Su Fei , Jia Liqun 
Published: May 2020

Abstract
Background
Diarrhea is a common adverse reaction in patients with cancer receiving chemotherapy, for which there is currently no effective method of treatment. Shengjiang Xiexin decoction (SXD), a classic traditional Chinese medicine (TCM) formula, has shown efficacy in alleviating irinotecan-induced diarrhea in preliminary clinical studies. The current study is designed to assess the efficacy and safety of SXD for prophylaxis against irinotecan-induced diarrhea. Additionally, we employ a new approach to analyze and evaluate the data based on the patients’ uridine diphosphate glucuronosyltransferase 1A1 (UGT1A1) genotype, which predicts the risk of diarrhea.

Methods and design
A prospective, double-blind, randomized, placebo-controlled trial will be conducted in patients with small cell lung cancer (SCLC) from five hospitals in China. For this study, 100 irinotecan-naïve patients will be randomly allocated to either the SXD or placebo arms in a 1:1 ratio. Stratified randomization will be used to divide subjects by UGT1A1 genotype into groups with differing risk of diarrhea. The trial will consist of two cycles of chemotherapy with 14 days of oral administration of SXD or placebo administered beginning between 3 days before and up to 11 days after initiation of each chemotherapy cycle. The primary study outcome is the incidence of diarrhea. Secondary outcomes include the degree of diarrhea, the degree of neutropenia, the rate of alterations in chemotherapy regimens, the amount of antidiarrheal drug taken, the rate of hospitalization, and evaluation of chemotherapy efficacy.

Discussion
This study is the first to use the UGT1A1 genotype to stratify patients into groups based on their risk of diarrhea, and to provide a complete assessment of chemotherapy-related diarrhea (CRD), including records of diarrhea duration, grading the severity of diarrhea, and evaluating concomitant symptoms. Study results will provide high-level clinical evidence on the use of SXD as prophylaxis for CRD.

Trial registration
Chinese Clinical Trial Register: ChiCTR1800018490. Registered on 20 September 2018. Retrospectively registered. http://www.chictr.org.cn/edit.aspx?pid=25250&htm=4c
Uncharted digenean diversity in Lake Tanganyika: cryptogonimids (Digenea: Cryptogonimidae) infecting endemic lates perches (Actinopterygii: Latidae)
Authors: Kmentová Nikol , Bray Rodney A. , Koblmüller Stephan , Artois Tom , De Keyzer Els Lea R. , Gelnar Milan , Vanhove Maarten P. M. , Georgieva Simona 
Published: May 2020

AbstractBackgroundLake Tanganyika is considered a biodiversity hotspot with exceptional species richness and level of endemism. Given the global importance of the lake in the field of evolutionary biology, the understudied status of its parasite fauna is surprising with a single digenean species reported to date. Although the most famous group within the lake’s fish fauna are cichlids, the pelagic zone is occupied mainly by endemic species of clupeids (Actinopterygii: Clupeidae) and lates perches (Actinopterygii: Latidae,LatesCuvier), which are an important commercial source for local fisheries. In this study, we focused on the lake’s four lates perches and targeted their thus far unexplored endoparasitic digenean fauna.MethodsA total of 85 lates perches from four localities in Lake Tanganyika were examined. Cryptogonimid digeneans were studied by means of morphological and molecular characterisation. Partial sequences of the nuclear28SrRNA gene and the mitochondrial cytochromecoxidase subunit 1 (cox1) gene were sequenced for a representative subset of the specimens recovered. Phylogenetic analyses were conducted at the family level under Bayesian inference.ResultsOur integrative approach revealed the presence of six species within the family Cryptogonimidae Ward, 1917. Three out of the four species ofLateswere found to be infected with at least one cryptogonimid species. Two out of the three reported genera are new to science. Low interspecific but high intraspecific phenotypic and genetic diversity was found amongNeocladocystisspp. Phylogenetic inference based on partial28SrDNA sequences revealed a sister group relationship for two of the newly erected genera and their close relatedness to the widely distributed genus AcanthostomumLooss, 1899.ConclusionsThe present study provides the first comprehensive characterisation of the digenean diversity in a fish family from Lake Tanganyika which will serve as a baseline for future explorations of the lake’s digenean fauna. Our study highlights the importance of employing an integrative approach for revealing the diversity in this unique host-parasite system.
Molecular characterisation and morphological description of two new species of Hepatozoon Miller, 1908 (Apicomplexa: Adeleorina: Hepatozoidae) infecting leukocytes of African leopards Panthera pardus pardus (L.)
Authors: van As Michelle , Netherlands Edward C. , Smit Nico J. 
Published: May 2020

Abstract
Background
The African leopard Panthera pardus pardus (L.) is currently listed as a vulnerable species on the IUCN (International Union for the Conservation of Nature) red list of threatened species due to ongoing population declines. This implies that leopard-specific parasites are also vulnerable to extinction. Intracellular apicomplexan haemoparasites from the genus Hepatozoon Miller, 1908 have been widely reported from wild carnivores in Africa, including non-specific reports from leopards. This paper describes two new haemogregarines in captive and wild leopards from South Africa and provides a tabular summary of these species in relation to species of Hepatozoon reported from mammalian carnivores.

Methods
Blood was collected from nine captive and eight wild leopards at various localities throughout South Africa. Thin blood smears were Giemsa-stained and screened for intraleukocytic haemoparasites. Gamont stages were micrographed and morphometrically compared with existing literature pertaining to infections in felid hosts. Haemogregarine specific primer set 4558F and 2733R was used to target the 18S rRNA gene for molecular analysis. Resulting sequences were compared to each other and with other available representative mammalian carnivore Hepatozoon sequences from GenBank.

Results
Two species of Hepatozoon were found in captive and wild leopards. Of the 17 leopards screened, eight were infected with one or both morphologically and genetically distinct haemogregarines. When compared with other species of Hepatozoon reported from felids, the two species from this study were morphometrically and molecularly distinct. Species of Hepatozoon from this study were observed to exclusively parasitize a particular type of leukocyte, with Hepatozoon luiperdjie n. sp. infecting neutrophils and Hepatozoon ingwe n. sp. infecting lymphocytes. Phylogenetic analysis showed that these haemogregarines are genetically distinct, with Hepatozoon luiperdjie n. sp. and Hepatozoon ingwe n. sp. falling in well supported separate clades.

Conclusions
To our knowledge, this is the first morphometric and molecular description of Hepatozoon in captive and wild African leopards in South Africa. This study highlights the value of using both morphometric and molecular characteristics when describing species of Hepatozoon from felid hosts.
Norm inequalities involving a special class of functions for sector matrices
Authors: Afraz Davood , Lashkaripour Rahmatollah , Bakherad Mojtaba 
Published: May 2020

AbstractIn this paper, we present some unitarily invariant norm inequalities for sector matrices involving a special class of functions. In particular, if Z=(Z11Z12Z21Z22) is a $2n\times 2n$2n×2n matrix such that numerical range of Z is contained in a sector region $S_{\alpha } $Sα for some $\alpha \in [0,\frac{\pi }{2} ) $α∈[0,π2), then, for a submultiplicative function h of the class $\mathcal{C} $C and every unitarily invariant norm, we have
$$\begin{aligned} \bigl\Vert h \bigl( \vert Z_{ij} \vert ^{2} \bigr) \bigr\Vert &\leq \bigl\Vert h^{r} \bigl( \sec (\alpha ) \vert Z_{11} \vert \bigr) \bigr\Vert ^{\frac{1}{r} } \bigl\Vert h^{s} \bigl( \sec (\alpha ) \vert Z_{22} \vert \bigr) \bigr\Vert ^{ \frac{1}{s} }, \end{aligned}$$ ∥h(|Zij|2)∥≤∥hr(sec(α)|Z11|)∥1r∥hs(sec(α)|Z22|)∥1s, where r and s are positive real numbers with $\frac{1}{r}+\frac{1}{s}=1 $1r+1s=1 and $i,j=1,2$i,j=1,2. We also extend some unitarily invariant norm inequalities for sector matrices.
Understanding stakeholder preferences for managing red foxes in different situations
Authors: Liordos Vasilios , Kontsiotis Vasileios J. , Emmanouilidou Foteini 
Published: May 2020

Abstract
Background
Red foxes (Vulpes vulpes) have historically been interacting with human societies, thus being an important component of socio-ecological systems. In Greece, farmers and hunters have been increasingly complaining about predation of red foxes on livestock and game, and the recurrence of rabies incidents has raised concerns about human and animal health. Understanding public preferences about management is necessary for successful wildlife management. This study aimed at investigating the preferences of north Greece residents for managing the negative impacts of red foxes and also at understanding variation between stakeholder groups: farmers, hunters, farmers-hunters, and the general public. Data were collected from on-site face-to-face surveys (n = 746), between March and May 2017. Respondents were asked to rate their acceptability of management strategies under three impact scenarios: red foxes attack livestock, reduce game, and carry rabies.

Results
Stakeholders preferred nonlethal management strategies, with generally high consensus, both between and within groups. Fencing and compensation were the most acceptable strategies for protecting livestock and vaccination and the removal of sick animals for eliminating rabies. Acceptability and consensus for lethal strategies were lower, with hunting being the most acceptable lethal strategy for hunters, especially when foxes threatened game or carried rabies. Doing nothing was becoming more unacceptable and lethal control more acceptable with increasing severity of the impacts, i.e., livestock and game predation versus rabies transmission.

Conclusions
Variation in the acceptability of and consensus for management strategies was considerable among scenarios, and both between and within stakeholder groups. Research implications could be used as a guide for reaching consensus for proper management strategies during the conservation conflict management process in the study area. As similar studies are scarce, findings might also prove useful elsewhere, especially in the northern hemisphere where native populations do occur. Findings about managing rabid red foxes would be particularly useful for countries where rabies has not been eliminated, particularly for neighboring Balkan and Asia Minor countries. Findings about managing red fox impacts on livestock and game would be most useful for European countries and especially Mediterranean countries with social and ecological conditions similar to Greece.
Mechanical and Thermal Properties of Interlocking Bricks Utilizing Wasted Polyethylene Terephthalate
Authors: Alaloul Wesam Salah , John Vivekka Olivia , Musarat Muhammad Ali 
Published: May 2020

AbstractPlastic bottles are non-biodegradable material made up of Polyethylene Terephthalate (PET) and takes around 450 years to get decomposed. In Malaysia, near 13.2% of plastics contribute to municipal solid waste, where 2.5% is PET. To reduce the waste, interlocking bricks manufacture by waste plastic bottles are used to replace the conventional bricks that use cement and clay. The purpose of this research is to reuse plastic bottles comprised of Polyethylene Terephthalate and Polyurethane binder, by manufacturing interlocking brick that helps to reduce the waste on landfills and the pollution. The plastic bottles were shredded and grinded to a size of 0.75 mm and mixed with the Polyurethane (PU) and the Polymer. The mixed later casted and compacted in the interlocking brick machine mould. The tests performed on the interlocking bricks were compressive strength, impact, flexural strength and thermal conductivity for obtaining the mechanical and thermal properties. The tests values were then keyed into the Response Surface Methodology (RSM) to obtain the optimal PET and PU to verify reliability. Based on the results it is concluded that PET/PU of 60/40 ratio is suitable as non-load bearing masonry brick and recommended to be used as partition walls.
The effects of vagus nerve stimulation on the course and outcomes of patients with bipolar disorder in a treatment-resistant depressive episode: a 5-year prospective registry
Authors: McAllister-Williams R. Hamish , Sousa Soraia , Kumar Arun , Greco Teresa , Bunker Mark T. , Aaronson Scott T. , Conway Charles R. , Rush A. John 
Published: May 2020

Abstract
Background
To compare illness characteristics, treatment history, response and durability, and suicidality scores over a 5-year period in patients with treatment-resistant bipolar depression participating in a prospective, multicenter, open-label registry and receiving Vagus Nerve Stimulation Therapy (VNS Therapy) plus treatment-as-usual (VNS + TAU) or TAU alone.

Methods
Response was defined as ≥ 50% decrease from baseline Montgomery–Åsberg Depression Rating Scale (MADRS) total score at 3, 6, 9, or 12 months post-baseline. Response was retained while MADRS score remained ≥ 40% lower than baseline. Time-to-events was estimated using Kaplan–Meier (KM) analysis and compared using log-rank test. Suicidality was assessed using the MADRS Item 10 score.

Results
At baseline (entry into registry), the VNS + TAU group (N = 97) had more episodes of depression, psychiatric hospitalizations, lifetime suicide attempts and higher suicidality score, more severe symptoms (based on MADRS and other scales), and higher rate of prior electroconvulsive therapy than TAU group (N = 59). Lifetime use of medications was similar between the groups (a mean of 9) and was consistent with the severe treatment-resistant nature of their depression. Over 5 years, 63% (61/97) in VNS + TAU had an initial response compared with 39% (23/59) in TAU. The time-to-initial response was significantly quicker for VNS + TAU than for TAU (p < 0.03). Among responders in the first year after implant, the KM estimate of the median time-to-relapse from initial response was 15.2 vs 7.6 months for VNS + TAU compared with TAU (difference was not statistically significant). The mean reduction in suicidality score across the study visits was significantly greater in the VNS + TAU than in the TAU group (p < 0.001).

Conclusions
The patients who received VNS + TAU included in this analysis had severe bipolar depression that had proved extremely difficult to treat. The TAU comparator group were similar though had slightly less severe illnesses on some measures and had less history of suicide attempts. Treatment with VNS + TAU was associated with a higher likelihood of attaining a response compared to TAU alone. VNS + TAU was also associated with a significantly greater mean reduction in suicidality.

Limitations
In this registry study, participants were not randomized to the study treatment group, VNS Therapy stimulation parameters were not controlled, and there was a high attrition rate over 5 years.
Trial registration ClinicalTrials.gov NCT00320372. Registered 3 May 2006, https://clinicaltrials.gov/ct2/show/NCT00320372 (retrospectively registered)
Inhibition of PI3K/AKT molecular pathway mediated by membrane estrogen receptor GPER accounts for cryptotanshinone induced antiproliferative effect on breast cancer SKBR-3 cells
Authors: Shi Danning , Zhao Piwen , Cui Lixia , Li Hongbo , Sun Liping , Niu Jianzhao , Chen Meng 
Published: May 2020

Abstract
Background
Breast cancer is the most frequently diagnosed malignancy among women and the second leading cause of cancer death worldwide. Among which nuclear estrogen receptor (nER) negative breast cancer is always with much poor prognosis. Recently, membrane G protein coupled estrogen receptor (GPER), a newly recognized estrogen receptor has been documented to take essential part in the development and treatment of breast cancer. The present study was designed to investigate the anti nER negative breast cancer effect of cryptotanshinone (CPT), an important active compound of traditional Chinese medicine Danshen and its possible molecular pathway.

Methods
The following in vitro tests were performed in nER negative but GPER positive breast cancer SKBR-3 cells. The effect of CPT on cell proliferation rate and cell cycle distribution was evaluated by MTT cell viability test and flow cytometry assay respectively. The role of PI3K/AKT pathway and the mediated function of GPER were tested by western blot and immunofluorescence. Technique of gene silence and the specific GPER agonist G-1 and antagonist G-15 were employed in the experiments to further verify the function of GPER in mediating the anticancer role of CPT.

Results
The results showed that proliferation of SKBR-3 cells could be blocked by CPT in a time and dose dependent manner. CPT could also exert antiproliferative activities by arresting cell cycle progression in G1 phase and down regulating the expression level of cyclin A, cyclin B, cyclin D and cyclin-dependent kinase 2 (CDK2). The antiproliferative effect of CPT was further enhanced by G-1 and attenuated by G-15. Results of western blot and immunofluorescence showed that expression of PI3K and p-AKT could be downregulated by CPT and such effects were mediated by GPER which were further demonstrated by gene silence test.

Conclusion
The current study showed that the antiproliferative action of CPT on SKBR-3 cells was realized by inhibition of GPER mediated PI3K/AKT pathway. These findings provide further validation of GPER serving as useful therapeutic target.
How to divide the pancreatic parenchyma in patients with a portal annular pancreas: laparoscopic spleen-preserving distal pancreatectomy for serous cystic neoplasms
Authors: Kuriyama Naohisa , Hatanaka Tomohide , Gyoten Kazuaki , Hayasaki Aoi , Fujii Takehiro , Iizawa Yusuke , Kato Hiroyuki , Murata Yasuhiro , Tanemura Akihiro , Kishiwada Masashi , Sakurai Hiroyuki , Mizuno Shugo 
Published: May 2020

Abstract
Background
Portal annular pancreas (PAP) is a rare pancreatic anomaly in which the uncinate process wraps annularly around the portal vein and fuses to the body of the pancreas. PAP is highly relevant to the development of postoperative pancreatic fistula (POPF) in pancreatic surgery. Here, we describe our experience and surgical technique of laparoscopic spleen-preserving distal pancreatectomy using Warshaw’s procedure for patients with the PAP.

Case presentation
A 49-year-old woman with PAP was referred to our hospital for a suspicious mucinous cystic neoplasms 1.5 cm in diameter in the pancreatic tail. Laparoscopic spleen-preserving distal pancreatectomy using Warshaw’s procedure was performed. Mobilization of the pancreatic tail was first performed, and then, the splenic artery was cut. After dividing the pancreatic tail from the splenic hilum, the ventral pancreatic parenchyma was divided using a stapler. After cutting the splenic vein, complete mobilization of the pancreatic body and tail enabled easy division of the PAP. Finally, the PAP was also divided using the stapler. Although grade B POPF occurred, she was discharged on the 9th postoperative day.

Conclusions
Surgeons should understand the anatomical characteristics of PAP and be aware of the possibility of POPF.
Additional cleft mitral valve diagnosed by a combination of 2-D and 3-D echocardiography using transesophageal echocardiography during mitral valve prolapse: a case report
Authors: Miyata Kazuto , Shigematsu Sayaka 
Published: May 2020

Abstract
Background
A mitral cleft may be an important etiological factor for significant mitral regurgitation. We diagnose an additional cleft mitral valve by a combination of 2-dimensional (2-D) and 3-dimensional (3-D) echocardiography.

Case presentation
We describe the case of a severe mitral regurgitation due to posterior leaflet prolapse (P2). In the 2-D view, which is obtained after turning the probe clockwise from the mid-esophageal long-axis view, TEE showed a moderate central regurgitation jet. In the 3-D en face view, a cleft between P2 and P3 was identified, and we found that the cause of mitral regurgitation was not only P2 prolapse but also a cleft between P2 and P3.

Conclusion
A complex mitral valve lesion was detected by a combination of 2-D and 3-D TEE. The presence of a cleft could affect the surgical procedure because of the possibility that an enlarged cleft would increase with leaflet resection.
Automated synthesis and quality control of [99mTc]Tc-PSMA for radioguided surgery (in a [68Ga]Ga-PSMA workflow)
Authors: Aalbersberg Else A. , van Andel Lotte , Geluk-Jonker Martine M. , Beijnen Jos H. , Stokkel Marcel P. M. , Hendrikx Jeroen J. M. A. 
Published: May 2020

Abstract
Background
Lymph node dissection is a therapeutic option for prostate cancer patients with a high risk of- or proven lymph node metastases. Radioguided surgery after intravenous injection of [99mTc]Tc-PSMA could improve the selectivity of lymph node dissection. The aim of this project was to develop an automated synthesis method for [99mTc]Tc-PSMA, using the disposables and chemicals used at our institute for [68Ga]Ga-PSMA labeling. Furthermore, quality control procedures and validation results of the automated production of [99mTc]Tc-PSMA conform cGMP and cGRPP are presented.

Methods
[99mTc]Tc-PSMA is produced fully automatic with a Scintomics synthesis module. Quality control procedures are described and performed for: activity, labeling yield, visual inspection, pH measurement, sterility and endotoxin determination, radionuclide purity, radiochemical purity (99mTc-colloids, unbound [99mTc]pertechnetate, and other impurities), and HEPES content. Three batches of [99mTc]Tc-PSMA were prepared on three separate days for validation and stability testing at 0, 4, 6, and 24 h.

Results
[99mTc]Tc-PSMA can be successfully manufactured automatically within a [68Ga]Ga-PSMA workflow with the addition of only [99mTc]pertechnetate and stannous chloride. The radiochemical purity after production was highly reproducible (96.3%, 97.6%, and 98.2%) and remained > 90% (required for patient administration) up to 6 h later.

Conclusion
A fully automated labeling procedure with corresponding quality control methods for production of [99mTc]Tc-PSMA is presented, which is validated according to cGMP and cGRPP guidelines and can be implemented in a GMP environment. The produced [99mTc]Tc-PSMA is stable for up to 6 h. The presented procedure is almost identical to the automated production of [68Ga]Ga-PSMA and can therefore be implemented expediently if a workflow for [68Ga]Ga-PSMA is already in place.