bluuebunny/crossref_metadata_2025_split huggingface dataset:
Balance of CD4<sup>+</sup>IFNγ+ and CD4<sup>+</sup>CD25<sup>hi</sup>T cells as early predictor of a 3-month outcome in ischemic stroke patients
Authors: Morozov S. A. , Tikhonova M. A. , Pronkina N. V. , Shtobbe A. A. , Leplina O. Yu. , Shevela E. Ya. , Ostanin A. A. , Chernykh E. R. 
Published: August 2020

Early prediction for ischemic stroke (IS) outcome is a major challenge since it may help to optimize treatment program and to make it more personalized. Since T cells with regulatory activity are involved in different pathophysiological processes in brain stroke, including inflammation, immune suppression, brain damage and repair, the study of T cells as potential biomarkers has essential importance. The present work aimed to study the circulating T cell subsets with phenotype of type 1 T helper cells (Th1) and regulatory T cells (Treg), and their ratio during the acute phase of IS, depending on stroke severity, inflammatory response and 3-month outcome (according to modified Rankin scale, mRs). Patients and methods. The study included 61 patients with a newly diagnosed IS (severity according to NIHSS ≥ 5), in the first 24-48 h after stroke onset, and 20 age/sex-related healthy donors. Laboratory examination included assessment of leukocytosis, neutrophillymphocyte ratio (NLR) and CRP concentration. Mononuclear cells were isolated from peripheral blood to study T cell subsets. Th1 and Tregs were measured by FACS analysis as CD4+IFNγ+ and CD4+CD25hiT cells, respectively. During the first 24-48 h after stroke, the patients had elevated values of leukocyte counts, NLR and CRP. Higher levels of these parameters in severe stroke compared with mild stroke, as well as direct correlation of NIHSS with NLR and CRP evidenced that the stroke severity was associated with more pronounced inflammatory response. Patients were also characterized by a significant decrease in CD4+IFNγ+Th1 cells, an increase in CD4+CD25hiTreg, and a marked decrease in Th1/Treg ratio. Furthermore, in patients with NIHSS ≥ 8 (moderate and severe stroke), the percentage of CD4+IFNγ+T cells was in direct correlation, and the number of CD4+CD25hiT cells was inversely related to CRP and NLR values. The changes of T cell subsets were more pronounced in patients with a favorable 3-month outcome (mRs > 3). As a result, the patients with poor outcome (mRs ≤ 3) had higher CD4+IFNγ+T cell proportion, lower CD4+CD25hiT cell percentage and 4-fold higher CD4+IFNγ+/CD4+CD25hi ratio compared with opposing group. ROC analysis revealed a “good” quality of prognosis based on evaluation of the CD4+IFNγ+/CD4+CD25hi ratio as a monopredictor of adverse outcome (AUC = 0.75) and “very good” quality of prognosis when the indicated ratio was combined with NIHSS scale (AUC = 0.82). The data obtained suggest that a decrease of Th1/Тreg ratio, due to a decrease in CD4+IFNγ+ and increased CD4+CD25hiT cell counts during the acute phase of ischemic stroke is a compensatory reaction directed at inhibition of inflammatory response, and has a prognostic significance as early predictor of the outcome at 3 months.
Development of ELISA test for the quality control of Pseudomonas aeruginosa recombinant vaccine based on the hybrid recombinant protein
Authors: Soldatenkova A. V. , Kudryashova A. M. , Gavrilova N. F. , Yakovleva I. V. , Borisova O. V. , Sviridov V. V. , Mikhailova N. A. 
Published: August 2020

A hybrid recombinant protein containing the amino acid sequences of the three most significant Pseudomonas aeruginosa antigens (membrane proteins OprF, OprI and toxoid aTox) was incorporated into a vaccine against Pseudomonas infection. Quality control of a hybrid recombinant protein and appropriate vaccine includes determination of authentity and completeness of adsorption upon aluminum hydroxide adjuvant. The aim of our study was to develop techniques of quality control for a vaccine based on the hybrid OprF-aToxOprI recombinant protein specific to P. aeruginosa. Hybridomas secreting specific monoclonal antibodies for OprF-aTox-OprI were derived from the fusion of myeloma cells and murine spleen cells immunized with recombinant proteins P. aeruginosa. To produce sufficient quantities of antibodies, the hybrid cells were in vivo cultured in BALB/c mice. Supernates and ascite liquids were chromatographically purified with immune sorbent. Conjugation of antibodies with horseradish peroxidase was carried out according to P.K.Nakane. The hybrid OprF-aTox-OprI recombinant protein was detected by the solid-phase ELISA, using a panel of monoclonal antibodies and conjugates of monoclonal antibodies with horseradish peroxidase. Monoclonal antibodies were specific for different OprF-aTox-OprI epitopes. Titration assays containing OprF-aTox-OprI protein at 78 ng/ml to 5000 ng/ml were used as quantitative standards for calibration curves.To identify the recombinant protein OprF-aTox-OprI, 55 variants of of MAb pairs were tested. Limits of quantitative detection served for selection of most sensitive and specific ELISA variants. The quantitative detection limit was calculated for all 11 ELISA variants. Two ELISA variants with the highest sensitivity were selected for quality control of the hybrid recombinant protein. The limits of quantitative detection were, respectively, 2.9 and 13.6 ng/ml (0.0058 and 0.027% of the estimated antigen content in the vaccine) for the first and second ELISA variants. The first variant included a pair of monoclonal antibodies specific for the OprF and OprI epitopes, the second variant represented aTox and OprI epitopes. Two variants of ELISA were developed to detect the hybrid recombinant OprF-aTox-OprI protein. The first variant allows to determine the protein amount and to evaluate completeness of its adsorption on aluminum hydroxide. To confirm authenticity of the protein, both methods must be used, since they can detect all three antigens (OprF, aTox and OprI) which are present in the fusion protein.
Polymorbidity, ageing of immune system and low-grade systemic inflammation: a challenge for modern medicine
Authors: Shirinsky V. S. , Shirinsky I. V. 
Published: August 2020

The review article considers the data from literature that concern polymorbidity aspects, its interrelations with ageing of immune system and lo-grade immune ageing, mechanisms of genesis, approaches to its prevention and treatment. Evolution of “comorbidity” and “polymorbidity” terms is traced, an updated definition of polymorbidity is proposed. The world-wide incidence of polymorbidity is increased and now it reaches 23-25% in general population, and up to 98%, in elderly people (> 65 years old). The risk factors of polymorbidity are considered, like as its social burden due to high costs for healthcare, high mortality rates, excessive treatment provided by multidisciplinary specialists. We present evidence for common molecular and cellular mechanisms involved in ageing and polymorbidity, being unified by the term “inflammaging” which represents a low-grade chronic systemic inflammation associated with ageing. The data are presented that concern the “inflammaging” development with involvement of ageing cells from innate and adaptive immunity systems, different pro and anti-inflammatory mediators, lifelong antigenic load. The data are analyzed concerning functional and structural changes in the inborn and adaptive immune system in ageing, role of these changes in “inflammaging” persistence and development of polymorbid conditions. There are complex interactions shown between the bodily senescence and immune ageing, with similar underlying mechanisms in some cases, however, being quite different in other instances. With age, upon existing risk factors, the changed adaptive immunity in most people is not able to full-scale coping with chronic antigenic load, thus increasing the risk of diseases. Moreover, in many elderly people these changes are compensated by steady activation of the innate immunity cells. It is noted that the aging events and development of disease (polymorbidity) cannot be considered distinct entities, since they can interact, being, however, basically different in their nature. In future, one should concentrate our efforts on elucidation of molecular and cellular mechanisms of these interactions, solution of the tasks oriented for development of such interventions that could be able to reduce harmful consequences of ageing and to use useful effects for health maintenance and reaching maximal longevity.
PENGEMBANGAN BAHAN AJAR MEDIA PEMBELAJARAN MATEMATIKA BERBASIS LECTORA INSPIRE DI SEKOLAH DASAR
Authors: Rostika Ika , Pamungkas Aan Subhan , Alamsyah Trian Pamungkas 
Published: August 2020

This research aims to develop teaching materials for mathematics learning media based on lectora inspire in elementary schools on the material of plane properties, to see the response and understanding of students. The subject of this study was the fifth grade students of SDN Pasir. Amounting to 30 students in the 2018/2019 academic year. Data collection is obtained by tests, questionnaires and documentation. The results of the study were (1) obtained an average score of expert validation of 84.15% with the category "feasible" (2) the average score of student responses with a percentage of 97.6% which was included in the "very good" category ( 3) post-test average score of 81,49% with the "very good" category so that it can be concluded that the teaching material of lectora inspire-based mathematics learning media is suitable to be used in fifth grade on plane properties and can provide understanding to studentsnin fifth grade plane properties.
PENGARUH PEMAHAMAN KURIKULUM 2013 DAN INTEGRITAS GURU TERHADAP KINERJA GURU-GURU SD KATOLIK DI MANADO
Authors: Sobon Kosmas , Mangundap Jelvi Monica , Walewangko Stief 
Published: August 2020

The purpose of this research is to evaluate and analyze the understanding of the curriculum 2013 and integration on the performance of Catholic elementary school teachers in Manado City. The total of samples are 76 respondents in 11 Catholic Primary Schools. This research is in June to July 2019. The method of the research is quantitative approach by survey methods. The results showed that (1) There is a positive influence of the understanding of the curriculum 2013 on the performance of Catholic elementary school teachers in Manado City. The effect of understanding of the curriculum 2013 on teacher performance was relatively low that is 39.1% with a significance level of 0.000 ≤ from α = 0.05. While the results of the hypothesis test knowledge variable with the t test shows the fact that t-count = 6.889 ≥ (t-tab) = 1.992. (2) There is effect of teacher’s integration on the performance of Catholic elementary school teachers with the results of the determination of percentage, 8.2% and a significance level of 0.012 ≤ from α = 0.05; (3) There is a effect of curriculum 2013 and integration simultaneously on the performance of Catholic elementary school teachers, 37.5% with a significance level of 0,000 ≤ from α = 0.05.
Prevalence of Left Ventricular Dysfunction and Relationship with Serum Selenium in Apparently Healthy Pregnant Women: Results from the PEACE Registry
Authors: Karaye Kamilu Musa , Mohammed Idris Y , Sa'idu Hadiza , Ishaq Naser A , Balarabe Suleiman A , Tukur Jamilu , Adedeji Tewogbade , Makinde Olufemiwa N , Adebayo Rasaaq A 
Published: August 2020

Background: The study aimed to determine the prevalence of left ventricular (LV) systolic and diastolic dysfunction in apparently healthy pregnant women and if there is relationship with serum selenium, in a society with high prevalence of peripartum cardiomyopathy (PPCM).Methods: This was a prospective longitudinal study carried out in 3 centres in Kano, Nigeria. 108 apparently healthy pregnant women were consecutively recruited between the 28th and 38th weeks of gestation and re-evaluated at the 6th to 8th weeks postpartum. Serum selenium was measured at enrolment during pregnancy. LV systolic dysfunction was defined as LV ejection fraction (LVEF) below 50%. Results: LV systolic dysfunction and diastolic dysfunction were found in 6 subjects (5.6%) and 20 subjects (18.5%) during pregnancy, and in 9 subjects (10.2%) (p=0.340) and 14 (15.9%) (p=0.631) after delivery, respectively. Mean LVEF was 62.2±6.9% and 60.6±8.2% (p=0.108) during pregnancy and after delivery respectively. Selenium deficiency (<70μg/L) was found in only 2.8% of subjects, and serum selenium did not significantly correlate with indices for LV systolic (LVEF) or diastolic (mitral E/e’ ratio) functions both during pregnancy and after delivery. Conclusion: LV systolic and diastolic dysfunction and selenium deficiency were uncommon during apparently healthy pregnancy and early puerperium, and serum selenium did not correlate with indices for LV function, in a population with high prevalence of PPCM.
A Perspective Study on Oral-fecal Transmission of COVID-19, its Prevention and Management
Authors: Abdullah Abdullah , Faisal Shah , Jan Hasnain , Zainab Rimsha , Khan Ayyaz , Rahman Anees ur , Bibi Baseerat , Ishaq Hania 
Published: August 2020

The novel coronavirus outbreak arose in Wuhan, China in Dec, 2019. It is declared the 6th public health emergency by the WHO and named as COVID-19. SARS-CoV-2 is non-segmented positive sense ssRNA virus, belongs to the Coronaviridae under the Nidovirales and spread largely in human being and other mammals. Person to person, airborne and surface transmission is common, the virus gets entered to host through nose, mouth, eyes, food, water and feces. An infected patient can transmit the virus to 2.2 healthy individuals. A patient in the US showed gastrointestinal symptoms vomiting, nausea and pass loose stools. Later the patient declared positive for SARS-CoV-2 based on viral detection in stools and respiratory samples. The gastrointestinal symptoms like diarrhea, nausea, vomiting, abdominal discomfort and detection of SARS-CoV-2 in stools of infected and recovered patients indicates potential oral-fecal transmission route, it could be potential risk for the spread of COVID-19. The flatus is gas produced by aerophagia or bacterial fermentation in intestine and expelled out through esophagus or anus. It consists of Hydrogen, Oxygen, Nitrogen, Carbon dioxide and Methane, their percentage composition is 99%. Previous study showed that bacteria can transmit through bare-bottom farting. The gastrointestinal manifestation and possible oral-fecal transmission, the flatulence could be the risk of transmission for COVID-19. Personal hygiene must be adapted to prevent the spread of disease.
LOS VÍNCULOS FAMILIARES Y EL AVANCE SOBRE LA FAMILIA DE CRIANZA
Authors: González Díaz Granados Jorge Luis , Parra Solano William José 
Published: August 2020

En este trabajo se elaborara un estudio que abarcará las antiguas normas procesales y sustanciales que tratan sobre los vínculos familiares, la evolución de los tipo de familia según la norma, jurisprudencia y el bloque constitucional y se analizará con base a los derechos fundamentales de las personas pertenecientes a los diferentes vínculos familiares; así mismo se realizará dentro de este artículo científico un estudio que abarcará un amplio margen que no es más que la generalidad del derecho sobre la filiación en un sentido amplio, vislumbrando los distintos y variados tipos de familia y se hará énfasis entre el vínculo familiar de crianza y los derechos de los cuales dichas familia de crianza a la fecha de realización de este trabajo científico poseen.
Impact of maternal factors and socio-demographic determinants on early initiation of breastfeeding practices in Alwar district, Rajasthan, India
Authors: Chand Harish , Sharma Jyoti 
Published: August 2020

Background: The SDG-3 goal suggests to reduce the neonatal mortality to below 12 per 1000 live births. New-born who are breastfed after 1 hour of birth are at 33% greater risk of neonatal mortality. To ascertain early initiation of breastfeeding (EIBF) knowledge, practices and its underlying determinants, a cross-sectional study was conducted with mothers of children below 3 years of age.Methods: Sample of 400 mothers of children under 3 years of age was collected. The data was analysed using statistical software SPSS version 23.0. All characteristics were summarized descriptively. Chi-square (χ2) test was performed to study the association between two categorical variables. Multivariable analysis was done using logistic regression model to determine the association of EIBF with socio-demographic and maternal factors. Results: 75.8% mothers initiated early breastfeeding within 1 hour of birth whereas only 34.3% mothers had knowledge on early initiation of breastfeeding. 95% mothers fed colostrum to the new-born. Mothers with 25 years of age or more (AOR 1, 95% CI 0.52, 1.57; p value <0.73) were less likely to breastfeed new-born within 1 hour of birth as compared to the mothers who were <25 years of age.Conclusions: Timely initiation of breastfeeding within 1 hour of birth is a critical intervention to avert a considerable number of neonatal deaths to achieve SDG-3 goal, which to reduce neonatal mortality rate <12 per 1000 livebirths. after delivery could sustain the breastfeeding practice for lo. Intensifying home visits in 3rd trimester to disseminate messages on EIBF and colostrum feeding may improve the coverage of EIBF.
Personal Data Protection in Indonesia: Legal Perspective
Authors: Nugroho Andriyanto Adhi , Winanti Atik , Surahmad Surahmad 
Published: August 2020

The arrangement of personal data protection in national law is stipulated explicitly in the regulation of the Minister of the Ministry of communication and Informasi No. 20 of 2016 on the protection of personal data. In Indonesia, there are no rules that accommodate the protection of personal data on financial technology. That can be hazardous when the economic response of technology is not limited to its use. The case of personal data is not contained in the case of a Such hazard that may arise. The case from the theft of personal data, damage to the system that may allow the occurrence of data breaches, misuse of personal data that has been the business ruled itself, or other parties who may access personal consumer data (such as government). The need to set this up is important because private data is a person's privacy right. Still, it can fundamentally be economically valuable for a third party who is about to take advantage of it.
Agency Valency of Voice Forms of the State Verbs (On the Material of the Uzbek Language)
Authors: Ravshankhodja Rasulov 
Published: August 2020

The article is devoted to the agent valence of voice forms of state verbs. It should be noted that, the expression of the relationship of movement, the state directed to the subject and object, is one of the main and important functions of the semantics of the verb. Voices totally cover the verbs. The verbs of state in the Uzbek language in their extra-linguistic substratum express certain processes connection with nature and society, flora and fauna, biology, physiology and psychology of human being. State verbs in the sentences are realized in the active, reflexive, compulsive, passive and mutual voices. State verbs with integral seme «duration», «result of movement», «execution», «movement», «skill», «imagery», «biological», «physiological» and «psychic» – are realized in speech mostly in the active voice. The agent of this verbs finds expression in the character of the object. The agent of some state verbs with the integral seme «result of movement», «execution», «imagery», «physiological» and «psychic» do not appear as functions of the object in sentence. Agent's non-objectivity, but only its subjectivity, follows from the transitivity of certain state verbs. The agent of some transitive state verbs with the integral seme «execution» and «psychic», even though they have own object, they are not only the producer (source) of the state, but also the object of the produced state. The possibility of combining state verbs with a direct object, that means, their transitiveness deserves special attention. State verbs with the integral seme «execution», «physiological» and «psychic» are realized in the sentence in a reflexive voice. Agent is an object in all cases. Verbs with the integral seme «duration», «result of movement», «movement», «biological», «physiological» and «psychic» are realized in speech in a compulsory voice. Agent here is not considered an object. State verbs with an integral seme «result of movement», «execution» and «movement» in a passive voice form have an agent valence. Here is shown the relationship object with state, which is the main, the most important, while agent is in secondary pose. The activity of the last one is weakening. This is the essence of the passive voice. State verbs with an integral seme «duration», «execution», «skill», «physiological» and «psychic» are realized in constructions in a mutual voice. The agent is revealed in the nature of the object.
Bilateral Adrenal Hemorrhage and Coagulation Laboratory Abnormalities: A Case Report of a Man with Enigmatic Clinical Features.
Authors: Lev Shaul , Ayda Gida , Sahaf levin Gal , Leader Avi , Bathish Jlal , Zeidman Aliza 
Published: August 2020

Background: bilateral adrenal hemorrhage can cause adrenal insufficiency. Antiphospholipid syndrome (APS) is considered a rare etiology of bilateral adrenal hemorrhage with a relatively small number of case reports. APS can be secondary to other autoimmune diseases such as SLE in which cardiac manifestations are common.
Case presentation: a fifty-four-year-old male presents with left flank pain as a result of unilateral adrenal hemorrhage, a few days later, bilateral hemorrhage is documented in the presence of abnormal coagulation state and other laboratory clues. APS diagnosis was established.
Coexisting chest pain accompanied by diffused ST elevation, long QT, slightly high troponin with a high pro-BNP, in addition to echo’s findings such abnormal regional wall abnormality and the absence of pericardial effusion made the case more interesting, differential diagnosis was proposed in accordance.
Conclusion: bilateral hemorrhage in APS considered rare. Requiring a high index of clinical suspicion. Particularly when it is the initial manifestation of APS as in our case. Underlying autoimmune diseases should be screened actively, especially when other unexplained manifestations are presented.