bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impact on Quality of Life 3 Years After Diagnosis of Prostate Cancer Patients Below 75 At Diagnosis: An Observational Case-Control Study
Authors: Houédé Nadine , Rébillard Xavier , Bouvet Sophie , Kabani Sarah , Fabbro-Peray Pascale , Trétarre Brigitte , Ménégaux Florence 
Published: July 2020

Abstract
Background: Prostate cancer patients are known to suffer from poor sexual and urinary long-term side-effects following treatment, potentially impacting quality of life. The purpose of our study was to compare health-related quality of life at 3 years between prostate cancer patients and healthy controls according to key life-style characteristics. Secondary objectives were to compare urological dysfunction, sexual function, anxiety and depression. Methods: Multicentric, case-control, observational prospective, open, follow-up study including 819 prostate cancer patients < 75 years old from the EPICAP cohort, newly diagnosed from 1 December 2011 to 31 March 2014 and 879 healthy controls. Participants were excluded if they experienced a relapse. Controls from the same geographical region were age-matched and were excluded if they were diagnosed with prostate cancer. Patients received one of the following treatments: active surveillance (AS), radical prostatectomy (RP), external beam radiotherapy (EBRT), High-intensity Focused Ultrasound (HIFU), chemotherapy (CT), or androgen deprivation therapy (ADT) as appropriate. The primary outcome was the quality of life as evaluated by the QLQ-C30 questionnaire. Scores were analyzed by multivariate analysis to adjust for predefined socio-demographic confounding effects. Results: In total, 564 participants were included (mean age 67.9 years): 376 patients and 188 controls. Treatment breakdown was: 258 underwent RP, 90 received EBRT, 52 brachytherapy or HIFU, 15 CT, 26 ADT and 61 AS. There was no difference in median global quality of life between patients and controls (94.87 vs 94.15, p=0.71). Multivariate analysis showed poorer social functioning in patients (24.3% vs. 16.3%, p=0.0209), more dyspnea (22% vs. 12.4%, p=0.0078), and yet less current pain (23% vs 33%, p=0.0151). Conclusions: Global health status score at 3 years after diagnosis was similar between patients and controls, though patients showed a significantly worse social functioning. Prostate cancer diagnosis per se does not impact the quality of life of patients <75 years at diagnosis. However, the therapeutic option that will be chosen following diagnosis should be carefully discussed with the medical staff in terms of benefit-risk ratios as it could have a long-term impact on urinary or erectile dysfunction.Trial registration: clinicaltrials.gov, NCT02854982. Registered 4 August 2016 – retrospectively registered, https://clinicaltrials.gov/ct2/show/NCT02854982
Measurement of salivary testosterone in adolescents and young men with Duchenne muscular dystrophy
Authors: Sahun Yolanda Alins , Cheetham Tim D , Boot Christopher , Straub Volker , Wood Claire L 
Published: July 2020

Abstract
BackgroundMany young adults with Duchenne Muscular Dystrophy (DMD) receive long-term glucocorticoids (GC). GC can cause hypogonadotrophic hypogonadism and adolescents may therefore be candidates for pubertal induction. It is unclear whether men with DMD on or off GC have age-appropriate endogenous testosterone production. MethodsWe undertook a quality improvement project to assess the feasibility of measuring salivary testosterone (SalT) levels in men with DMD at home. A Sal-T sampling kit was sent by post to all patients with DMD, aged 17 and older, registered at the John Walton Muscular Centre in Newcastle (n=75). Submitted Sal-T samples were collected and submitted for analysis. Results28 out of 75 patients returned samples (age range: 17-34 years). 6/28 samples were unsuitable for analysis. Overall Sal-T levels (n=22) were significantly lower than in the healthy population (178 ±107 v 287 ±109 pmol/l, p=0.0001). Sal-T was lower in those on GC compared to those off GC (144± 81 versus 218 ±125 pmol/l, p=0.05). 3 patients were unable to collect a sample due to ventilator dependence.ConclusionSal T can provide a useful method of assessing androgen status in DMD patients at home, overcoming barriers such as mobility difficulties or venepuncture. However we only obtained samples in a minority of patients suggesting that Sal-T measurement may not be appropriate or acceptable to everyone. There needs to be a more detailed exploration of the barriers to sample submission and a greater understanding of the implications of testosterone status on physical and psychological health in DMD patients.
BNIP3 decreases the LPS-induced inflammation and apoptosis of chondrocytes by promoting the development of autophagy
Authors: Ma Zetao , Wang Deli , Weng Jian , Zhang Sheng , Zhang Yuanshi 
Published: July 2020

Abstract
Background: Inflammation and apoptosis of chondrocytes are the pathological basis of osteoarthritis. Autophagy could alleviate the symptoms of inflammation and apoptosis. Previous study has shown that BCL2/adenovirus E1B 19 kDa protein-interacting protein 3 (BNIP3) can induce the occurrence and development of autophagy. However, it is unknown whether autophagy induced by BNIP3 can alleviate the inflammation and apoptosis of chondrocytes. Methods: We used the lentivirus to construct the overexpression BNIP3 chondrocytes. Next, the lipopolysaccharide (LPS) was used to stimulate these cells to simulate the physiological environment of osteoarthritis. After that, the enzyme-linked immunosorbent assays (ELISA) were performed to determine the levels of tumor necrosis factor-α (TNF-α), interleukin-1 beta (IL-1β) and interleukin-6 (IL-6) and the flow cytometry was performed to detect the apoptosis rates of chondrocytes. At last, the expression of autophagy related proteins was detected with the western blotting. Results: The expression of BNIP3 was suppressed after treatment with LPS. However, overexpression of BNIP3 inhibited the secretion of proinflammatory factors (TNF-α, IL-1β and IL-6) and decreased the apoptosis of chondrocytes. Furthermore, overexpression of BNIP3 led to the upregulation of autophagy related proteins expression including little computer 3 (LC3), autophagy-related protein 7 (ATG7) and Beclin-1. Application of autophagy inhibitor recovered the expression of proinflammatory factors and apoptosis rates of chondrocytes. Conclusions: BNIP3 decreased the LPS induced inflammation and apoptosis of chondrocytes by activating the autophagy.
Deep learning-based framework for the distinction of membranous nephropathy: A new approach through hyperspectral imagery
Authors: Tu Tianqi , Wei Xueling , Yang Yue , Zhang Nianrong , Li Wei , Tu Xiaowen , Li Wenge 
Published: July 2020

Abstract
Background

Common subtypes seen in Chinese patients with membranous nephropathy (MN) include idiopathic membranous nephropathy (IMN) and hepatitis B virus-related membranous nephropathy (HBV-MN). However, in some cases, the morphologic differences are not visible under the light microscope in the renal biopsy tissue.

Methods

We proposed a deep learning-based framework for processing hyperspectral images of renal biopsy tissue to define the difference between IMN and HBV-MN based on the component of their immune complex deposition.

Results

The proposed framework can achieve an overall accuracy of 95.04% for multiclass classification, which also proven to obtain a better performance compared to the support vector machine (SVM)-based algorithms.

Conclusion

IMN and HBV-MN could be correctly separated via the deep learning framework using hyperspectral imagery. Our results suggest the potential of the deep learning algorithm as a new method to aid the diagnosis process of MN.
The Relationship Between Self-Harm and Bullying Behavior: Results from a Population Based-Study of Adolescents
Authors: Myklestad Ingri , Straiton Melanie Lindsay 
Published: July 2020

Abstract
Background: This study aims to better understand the link between bullying behaviour (both the bullied, the bullies and the bully-victims) and self-harm, and which risk- and protective factors will moderate this link. Methods: A total of 16 182 adolescents, aged 12 to 19 years were invited to participate in the cross-sectional Ung-data survey. The response rate was 87%. Logistic regression analyses were used to investigate the relationship between self-harm and bulling behaviour, and which potential risk- and protective factors would partly explain this relationship. In addition, to test for potential interaction effects between protective factors and the three bullying groups on self-harm.Results: Fifteen percent of participating adolescents had engaged in self-harm in the last year. The risk for self-harm was six times higher for the “bully-victims”, five times higher for the bullied, and three times higher for the bullies, compared to the non-bullied nor bullies. The risk of self-harm in the face of being bullied was significantly greater for girls than boys, compared with their “neither being bullied nor bullying” counterparts. Depression and family conflicts explained part of the relationship between being bullied and self-harm, and between bully-victims and self-harm. School behaviour problems was an important risk factor for the bullies and bully-victims on self-harm. The relationship between self-harm and the bullied was significantly moderated by parental support and school well-being, and the relationship between self-harm and “bully-victims” was moderated by school well-being. Conclusion: Parental support and school well-being moderate the harmful consequences of bullying behaviour on self-harm among adolescents. The finding warrants further attention in future studies and interventions.
Gender-specific Prevalence and Influencing Factors of Ideal Cardiovascular Health in Chinese Rural Population: The Henan Rural Cohort Study
Authors: Wu Xueyan , Liu Xiaotian , Luo Zhicheng , Niu Miaomiao , He Yaling , Hou Jian , Mao Zhenxing , Huo Wenqian , Li Linlin , Wang Chongjian 
Published: July 2020

Abstract
Background: The American Heart Association define a new concept of ideal cardiovascular health (ICH), which was widely used. However, the prevalence of ICH according to the definition of Chinese rural population is unclear. The study aimed to estimate prevalence and influencing factors of ICH in rural areas of China.Methods: This study included 35081 participants (13711 men and 21370 women)aged 18 to 79 years from “the Henan Rural Cohort study”. The cardiovascular health (CVH) metrics, the ICH scores, the ideal health behaviors (IHB) scores and ideal health factors (IHF)scores were evaluated in Chinese rural adults. Each CVH metrics divided into ideal and non-ideal including diet, physical activity, smoking, BMI, blood pressure, total cholesterol and fasting plasma glucose. The influencing factors of ideal CVH (ICH scores≥5), ideal HB (IHB scores>3) and ideal HF (IHF scores>3) were explored by logistic regression analysis.Results: The age-standardized mean scores of the ICH was 4.30(3.62 in men and4.69 in women). Prevalence of ICH metrics about ideal physical activity was the highest (91.37%), while about ideal diet was lowest (0.48%).The age-standardized prevalence of population who with 7 scores of ICH was 0.10% (0.07% in men and 0.11% in women). The age-standardized prevalence of Ideal CVH (ICH scores≥5) was 48.86% (28.26% in men and 60.70% in women). The age-standardized prevalence of 4 of IHB scores and 4 of IHF scores were 0.16% (0.17% in men and 0.16% in women) and 33.26% (10.06% in men and 46.60% in women), respectively. Further, apart from IHB scores, age had an inverse relationship with the ICH scores and IHF scores in total populations and women. Much older, men, low family income, current drinking and abdominal obesity were independently associated with ICH in Chinese rural adults.Conclusions: The percentage of ideal cardiovascular health is extremely low in rural China. There is an increasing need for primordial prevention of unhealthy lifestyles and monitoring high risk factors to promote cardiovascular health in rural China.Clinical Trial Registration The Henan Rural Cohort Study has been registered at Chinese Clinical Trial Register (Registration number: ChiCTR-OOC-15006699). Date of registration: 2015-07-06.http://www.chictr.org.cn/showproj.aspx?proj=11375
Economic Impact of Demonetization: Critical Analysis on Social Impact in India
Authors: Raju Valliappan 
Published: July 2020

Abstract
This research paper discusses the general effects of
demonetisation on the economy with the notion of its influence in
growth. In the research study, 3 important independent variables
are selected that have influences towards the performance of
demonetization in India. Indian economy took a momentous shift of
banning high denomination notes calculated as 87 percent of total
currency in November 2016. The independent variables are social
impact, political impact and economic impact. These selected
independent variables are the possible factors that might influence
the performance of demonetisation recently happened in India. In
this research study, 184 sets of questionnaires were prepared and
distributed to the targeted respondents who are affected through
this, and one more thing the target population is from the
southwest part of India called Andhra Pradesh. After the data were
collected, IBM SPSS was used to testing the data in order to
generate the final result. In the end, the final result shows that
there is significant effect on socially, politically and
economically towards the performance of demonetisation.
The Impact of COVID-19 on Gastric Cancer Surgery: A Single-center Retrospective Study Based on Real-world Data from China
Authors: Li Yu-xuan , He Chang-zheng , Liu Yi-chen , Zhao Peng-yue , Xu Xiao-lei , Wang Yu-feng , Xia Shao-you , Du Xiaohui 
Published: July 2020

Abstract
Backgrounds:
A respiratory epidemic defined as coronavirus disease 2019 (COVID-19) is becoming unstoppable and has been declared a pandemic. Patients with cancer are more likely to develop COVID-19. Based on our experience during the pandemic period, we propose some surgery strategies for gastric cancer patients under the COVID-19 situation.
Methods
We defined the ‘COVID-19’ period as occurring between 2020-01-20 and 2020-03-20. All the enrolled patients were divided into two groups, pre-COVID-19 group (PCG) and COVID-19 group (CG). A total of 109 patients with gastric cancer were enrolled in this study.
Results
The waiting times before admission increased by 4 days in CG(PCG:4.5 [IQR: 2, 7.8] vs. CG:8.0 [IQR: 2,20]; P = 0.006). More patients had performed chest CT scan besides abdominal CT before admission during COVID-19 period(PCG:22[32%]vs. CG:30[73%], p = 0.001). After admission, during COVID period, the waiting time before surgery was longer(3[IQR: 2,5] vs. 7[IQR: 5,9]; P < 0.001),more laparoscopic surgery were performed(PCG: 51[75%] vs. CG: 38[92%],p = 0.021), and hospital stay after surgery was longer (7[IQR: 6,8] vs.9[IQR:7,11] ; P < 0.001). The total cost of hospitalization increased during COVID period, (9.22[IQR:7.82,10.97] vs. 10.42[IQR:8.99,12.57]; p = 0.006).
Conclusion
Since no data is available yet on the impact of COVID-19 on gastric cancer patients,our own experience with COVID-19 in gastric cancer surgery has hopefully provided an opportunity for colleagues to reflect on their own service and any contingency plans they have to tackle the crisis.
Impact of DNA methylation on 3D genome structure
Authors: Buitrago Diana , Labrador Mireia , Arcon Juan Pablo , Lema Rafael , Flores Oscar , Esteve-Codina Anna , Blanc Julie , Villegas Nuria , Bellido David , Gut Marta , Dans Pablo , Heath Simon , Gut Ivo , Heath Isabelle Brun , Orozco Modesto 
Published: July 2020

Abstract
The extreme complexity of epigenetic regulation in higher organisms makes the determination of the intrinsic effect of DNA methylation in chromatin structure and function challenging. We investigated the role of DNA methylation in a simpler model system, budding yeast (Saccharomyces cerevisiae), an organism in which methylation-related machinery is normally absent thus making it a perfect model system to study the intrinsic role of methylation in chromatin structure and function. With this aim, we expressed the murine DNA Methyl Transferases in S. cerevisiae and analyzed the correlation between DNA methylation, nucleosome positioning, gene expression and 3D genome organization. We showed that despite the lack of machinery for positioning and reading of methylation marks, the methylation pattern follows a conserved pattern, the level of DNA methylation being very low at the 5’ end of the gene, and then increasing gradually toward the 3’ end, mimicking mammalian behavior. DNA methylation and gene expression correlate as DNA methylation is lower at the TSS and higher at the TTS in highly expressed genes compared to lowly expressed ones, mimicking again mammalian behavior. We found that methylated DNA is unlikely to be wrapped around nucleosomes, but is concentrated in linkers and nucleosome free regions. DNA methylation increases chromatin condensation in the peri-centromeric region, decreases overall DNA flexibility and favors the heterochromatin state. Taken together, these results demonstrate that methylation intrinsically modulates chromatin structure and function even in the absence of cellular machinery evolved to recognize and process the methylation signal.
Interfacial Barrier Free Organic-Inorganic Hybrid Electrolytes for All Solid-State Batteries
Authors: Lee Myeong Ju , Shin Dong Ok , Kim Ju Young , Oh Jimin , Kim Jumi , Kang Seok Hun , Kim Kwang Man , Kim Sang Ouk , Lee Young-Gi 
Published: July 2020

Abstract
Organic-inorganic hybrid solid electrolytes (HSEs) are expected to overcome the inherent limitations of rigid fragile inorganic electrolytes for solid state batteries. Li-ion conductive filler such as garnet Li7La3Zr2O12 (LLZO) is proposed for the high performance of HSEs, unfortunately, which suffers from native surface layer resistance to Li-ion transport. Here we present highly conductive polyvinylidene fluoride (PVDF)-based HSEs incorporating LLZO fillers, whose resistive barriers are eliminated by dry etching. Our optimal composition of etched LLZO fillers (30 wt%) leads to ionic conductivity of 4.05 x 10-4 S cm-1, about two-fold improvement from non-etched counterpart. Li symmetric cells with etched fillers exhibit low interfacial resistance of 110 Ω cm2 and minimal overpotential of 46 mV. Moreover, high capacity of 79 mA h g-1 is highlighted at 4C, comparable or superior to liquid electrolyte or sulfide-based electrolyte devices. Interfacial environment in HSEs ideally modified for Li-ion transport is identified by 7Li NMR measurements.
Curcumin improves human umbilical cord derived mesenchymal stem cells survival and promotes motor outcome via ERK Signaling after spinal cord injury
Authors: Wanjiang Wu , Xin Chen , Yaxing Chen , Jie Wang , Hongyan Zhang , Fei Ni , Chengmin Ling , Chengjian Feng , Yuan Jichao , Jiangkai Lin 
Published: July 2020

Abstract
Background

Human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) transplantation are assumed as a promising strategy in spinal cord injury (SCI). However, the complex pathological microenvironment after SCI induces the apoptosis of hUC-MSCs, which limits the clinical application for the cell replacement therapy.
Methods

In this study, in order to investigate whether combined with curcumin could strengthen the therapeutic effects of hUC-MSCs transplantation for SCI, we mediated the apoptosis of hUC-MSCs with TNF-α and transplanted hUC-MSCs into SCI rats, followed by assessed the anti- apoptosis effect and mechanism of curcumin.
Results

LDH release test and flow cytometry demonstrated that TNF-α led to the hUC-MSCs apoptosis and curcumin increased survival rate of hUC-MSCs with dose-dependent. In addition, we showed that the phosphorylation levels of ERK, JNK and P38 were up-regulated in the hUC-MSCs apoptosis, while curcumin strengthened the phosphorylation of ERK, but not activated the JNK and P38, which was reversed by p42/44 antagonist U0126. Furthermore, we exhibited that the motor function scores and surviving HNA-positive cells were significantly increased after curcumin combined with hUC-MSCs transplantation therapy 8 weeks post SCI, while U0126 markedly attenuated these phenomenons.
Conclusions

The aforementioned data confirmed that curcumin suppressed the apoptosis of hUC-MSCs through ERK signal pathway and combined curcumin with hUC-MSCs treatment improved motor function after SCI in rats. The current research provides a strong basis for hUC-MSCs replacement therapy in conjunction with curcumin in the treatment and management of SCI in human.
Changes in the Gut Microbiome Community of nonhuman primate following radiation injury
Authors: Kalkeri Raj , Walters Kevin , Pol William Van Der , McFarland Braden C. , Fisher Nathan , Koide Fusataka , Morrow Casey D. , Singh Vijay K. 
Published: July 2020

Abstract
Background
Composition and maintenance of the microbiome is vital to gut homeostasis. However, there is limited knowledge regarding the impact of high doses of radiation, which can occur as a result of cancer radiation therapy, nuclear accidents or intentional release of a nuclear or radioactive weapon, on the composition of the gut microbiome. Therefore, we sought to analyze alterations to the gut microbiome of nonhuman primates (NHPs) exposed to high doses of radiation.
Methods
Fecal samples were collected from 19 NHPs (Chinese rhesus macaques, Macaca mulatta) one day prior and one and four days after exposure to 7.4 Gy cobalt-60 gamma-radiation (LD70 − 80/60). The 16S V4 rRNA sequences were extracted from each sample, followed by bioinformatics analysis using the QIIME platform.
Results
Alpha Diversity (Shannon Diversity Index), revealed no major difference between pre- and post-irradiation, whereas Beta diversity analysis showed significant differences in the microbiome after irradiation (day + 4) compared to baseline (pre-irradiation). The Firmicutes/Bacteriodetes ratio, a factor known to be associated with disruption of metabolic homeostasis, decreased from 1.2 to less than 1 post-radiation exposure. Actinobacillus, Bacteroides, Prevotella (Paraprevotellaceae family) and Veillonella genera were significantly increased by more than 2-fold and Acinetobacter and Aerococcus genus were decreased by more than 10-fold post-irradiation. Fifty-two percent (10/19) of animals exposed to radiation demonstrated diarrhea at day 4 post-irradiation. Comparison of microbiome composition of feces from animals with and without diarrhea at day 4 post-irradiation revealed an increase in Lactobacillus reuteri associated with diarrhea and a decrease of Lentisphaerae and Verrucomicrobioa phyla and Bacteroides in animals exhibiting diarrhea.
Conclusion
Our findings demonstrate that substantial alterations in the microbiome composition of NHPs occur following radiation injury and provide insight into early changes with high-dose, whole-body radiation exposure. Future studies will help identify microbiome biomarkers of radiation exposure and develop effective therapeutic intervention to mitigate the radiation injury.