bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Influence of Experiential Knowledge and Societal Perceptions on Decision-Making Regarding Non-Invasive Prenatal Testing (NIPT)
Authors: Montgomery Sophie , Thayer Zaneta M. 
Published: January 2020

Abstract
Non-invasive prenatal testing (NIPT) allows women to access genetic information about their fetuses without the physical risk inherent to prior testing methods. The advent of NIPT technology has yielded concerns among bioethicists regarding the quality and process of informed consent, as the routinization of this technology could degrade the intentionality of women choosing whether to undergo testing. Prior studies evaluating the NIPT decision-making process have focused on the clinical encounter as the primary environment for acquisition of biomedical information and decision formation. While important, this conceptualization fails to consider how additional sources of knowledge, including both embodied and empathetic experiential knowledge, shape perceptions of risk and the societal use of NIPT.
In order to address this issue, qualitative, semi-structured interviews with 25 women who had been offered NIPT were performed. Women were categorized by NIPT use/non-use, as well as whether their described decision-making process was routinized. Qualitative analysis of the data using a phenomenological approach was used to explore themes in the data, develop a framework of NIPT decision-making, and compare the perceptions of women with differential decision-making processes and outcomes.
A framework for decision-making regarding NIPT was developed based on three emergent factors: perceptions of the societal use of NIPT, expected emotional impact of genetic information, and perceived utility of genetic information. Qualitative analysis revealed that perceptions of widespread use of NIPT, pervasive societal narratives of NIPT use as progressive and “forward-thinking,” and a perception of information as anxiety-relieving contributed to routinized uptake of NIPT. In contrast, women who displayed a lack of routinization expressed fewer stereotypes regarding the audience for NIPT and relied on communication with their social networks in-person and online to consider how they might use the information provided by NIPT.
The findings of this study reveal the societal narratives and perceptions that shape differential decision-making regarding NIPT. Understanding and addressing these perceptions that influence NIPT decision-making, especially routinized uptake of NIPT, is important as the use and scope of this technology increases.
Radial peripapillary capillary density in superior segmental optic hypoplasia measured with OCT angiography.
Authors: Abe Maiko , Omodaka Kazuko , Kikawa Tsutomu , Nakazawa Toru 
Published: January 2020

Abstract
Background: To investigate the diagnostic power of radial peripapillary capillary (RPC) density, measured with optical coherence tomography angiography (OCT-A), in patients with superior segmental optic hypoplasia (SSOH).
Methods: Forty subjects with SSOH and 40 age- and axial length-matched control subjects were retrospectively registered for this study. SSOH was defined as intraocular pressure less than 21 mmHg with the presence of two of the following: superior rim thinning, superior entrance of the central retinal artery, scleral halo, and pale optic disc; as well as non-progressive visual field loss. RPC density was measured with swept-source OCT-A (Triton, Topcon) overall, in the quadrants, and in the 12 clock-wise sectors. Changes in RPC density were also compared in SSOH patients and age-matched patients with mild- or moderate-stage of glaucoma. RPC density was compared in pairs of groups with Welch’s t-test. Diagnostic power was assessed with the area under the receiver operating characteristics curve (AUC).
Results: Overall cpRNFLT was significantly different in the normal (106.7 ± 9.5 μm) and SSOH (77.2 ± 13.7 μm, p < 0.001) subjects. RPC density overall and in the superior, nasal, and inferior quadrants was significantly lower in the SSOH group (all, p < 0.001), but not in the temporal (p = 0.756) quadrant. The diagnostic power of RPC density was highest in the superior quadrant (AUC = 0.928) and the 1 o’clock sector (0.896). Comparing the SSOH and glaucoma patients showed that there were no significant differences in RPC density either overall (p=0.391) or in the superior quadrant (p = 0.268), while RPC density was significantly higher in the inferior (p = 0.005) and temporal quadrants (p < 0.001) and lower in the nasal quadrant (p = 0.029).
Conclusions: Low RPC density was found in the three non-temporal quadrants of the optic nerve head in SSOH patients, in comparison to normal subjects. Regionally, RPC density in SSOH was lower in the nasal quadrant and higher in the inferior and temporal quadrants in comparison to glaucoma patients. Measuring RPC density with OCT-A may help the diagnosis of SSOH and may improve the management of glaucoma.
Application of Freire’s Adult Education Model for Modifying the Psychological Constructs of Health Belief Model in Self-medication Behaviors of Elderly: a randomized controlled trial
Authors: Bakhtiar Katayon , Ardalan Arash , Araban Marzieh , Ebrahimzadeh Farzad , Almasian Mohammad , bastami Fatemeh 
Published: January 2020

Abstract
Background: As people age, they are more likely to engage in self-medication and suffer from its adverse effects. The present study aimed to modifying knowledge, psychological constructs of Health Belief Model (HBM), and self-medication behaviors using Freire’s Adult Education Model (FAEM) among elderlies in Khorramabad, Iran, from 2017 to 2018. Methods: This research was of a prospective randomized controlled trial conducted on 132 elderly individuals older than 60 who were referred from health care centers. The participants were selected using multistage sampling method and randomly divided into two groups of intervention and control. The data collection instruments included a questionnaire which was designed based on both HBM and the self-medication behaviors questionnaires. The phases of the adult education approach were used to modify the psychological constructs of HBM and self-medication behaviors. Data were analyzed using SPSS software version 20 with a significance level of 0.05. Descriptive statistical tests, the chi-squared test, paired t-test, independent t-test, and univariate modeling were employed for this purpose. Results: The mean age of the elderly was 66.28 ± 7.18 years. There was no significant difference between groups in terms of self-medicating. Unawareness of the effects of medicines were the most important causes of self-medication (p=0.50). The two groups were not significantly different in terms of knowledge, HBM constructs, and self-medication behaviors (p>0.05). However, they came up to be considerably different for the above variables after the intervention was completed (p<0.05). When the findings were adjusted for the effects of confounding variables, there were significant differences between almost all constructs of HBM and their behaviors (p < 0.05). However, the perceived barrier modality of HBM did not reach to a significant level of difference between two groups. Conclusion: The psychological constructs of HBM were affected at the phases of listening to the problem and reflecting on it. Self-medication was tempered at the action-reflection phase with shared creation and evaluation of the action plan geared towards the achievement of the behavioral objectives. The results might be of importance for healthcare professionals involved incare of older patients.
Cold priming uncouples light- and cold-regulation of gene expression in Arabidopsis thaliana
Authors: Bittner Andras , Buer Jörn van , Baier Margarete 
Published: January 2020

Abstract
Background The majority of stress-sensitive genes responds to cold and highlight in the same direction, if plants face the stresses for the first time. As shown recently for a small selection of genes of the core environmental stress response cluster, pre-treatment of Arabidopsis thaliana with a 24 h long 4 °C cold stimulus modifies cold regulation of gene expression for up to a week at 20 °C, although the primary cold effects are reverted within the first 24 h. Such memory based regulation is called priming. Here, we show the effect of 24 h cold priming on cold regulation of gene expression on a transcriptome-wide scale and test if and how cold priming effects light regulation of gene expression. Results 304 genes were differently regulated between cold-primed and non-primed plants after a second 24 h long 4 °C cold treatment. After triggering the plants with a heat-filtered highlight stimulus (800 µmol quanta m -2 s -1 ), 1011 genes showed priming dependent regulation. Only 32 of the priming-sensitive genes responded similarly to cold and light triggering. The majority of the priming-sensitive genes were regulated in a stressor-specific manner. 29 genes were even inversely regulated by the two triggering stimuli. Cold priming preferentially supported expression of genes involved in the defense against plant pathogens upon cold triggering. The regulation took place on the cost of the expression of genes involved in growth regulation and transport. On the contrary, cold priming resulted in stronger expression of genes regulating metabolism and development and weaker expression of defense genes in response to highlight triggering. qPCR in several independently cultivated and treated samples confirmed the trends observed by RNA-Sequencing. Conclusion The 24 h long priming cold stimulus activates a several day lasting stress memory that controls cold and light regulation of gene expression and adjusts growth and defense regulation in a stressor-specific manner.
Detection of CNV in the SH3RF2 gene in chickens and its effects on growth and carcass traits
Authors: Jing Zhenzhu , Wang Xinlei , Cheng Yingying , Wei Chengjie , Hou Dan , Li Tong , Li Wenya , Han Ruili , Li Hong , Sun Guirong , Tian Yadong , Liu Xiaojun , Kang Xiangtao , Li Zhuanjian 
Published: January 2020

Abstract
Background﻿SH3RF2 gene was a protein-coding gene located in the quantitative trait locus associated with body weight , and its deletion was positively correlated with body weight in chickens.ResultsIn the present study, the SH3RF2 genotype of the “Gushi ×Anka” F2 resource population , as well as local chickens and commercial layers and broilers in China was detected , and the relationship between the genotype and chicken production traits was further studied by molecular biology technology. The results showed that the local chickens and commercial layers were all homozygous for the wild-type allele. In commercial broiler breeds, deletion mutation individuals were detected in all commercial broiler breeds except Hubbard breeds.In addition , 798 individuals in the F2 resource group were used to analyze the effects of different genotypes (DD\ ID\ II) on chicken production traits. The results showed that the mutation was associated with 2 , 6 , 10 , and 12-week body weight and a significant correllation with 8-week breast bone length (P<0.05);The mutation was significantly correlated with 8-week body weight and 4-week breast bone length (P<0.01).Additionally , evisceration weight , legs muscle weight , carcass weight and other significant correlations (P<0.05) and breast muscle weight and gizzard weight were significantly correlated (P<0.01).ConclusionsThe results of this study indicated that the copy number variation of the SH3RF2 gene contributed to the growth and weight gain of chickens.
How Are Systematic Reviews of Prevalence Conducted? A Methodological Study
Authors: Migliavaca Celina Borges , Stein Cinara , Colpani Verônica , Barker Timothy Hugh , Munn Zachary , Falavigna Maicon 
Published: January 2020

Abstract
Background There is a notable lack of methodological and reporting guidance for systematic reviews of prevalence data. This information void has the potential to result in reviews that are inconsistent and inadequate to inform healthcare policy and decision making. The aim of this meta-epidemiological study is to describe the methodology of recently published prevalence systematic reviews.Methods We searched MEDLINE (via PubMed) from February 2017 to February 2018 for systematic reviews of prevalence studies. We included systematic reviews assessing the prevalence of any clinical condition using patients as the unit of measurement and we summarized data related to reporting of and methodology of the reviews.Results A total of 235 systematic reviews of prevalence were analyzed. The median number of authors was 5 (interquartile range [IQR] 4-7), the median number of databases searched was 4 (3-6) and the median of studies included in each review was 24 (IQR 15-41.5). Search strategies were presented for 68% of reviews. Forty five percent of reviews received external funding, and 24% did not provide funding information. Twenty three percent of included reviews had published or registered the systematic review protocol. Reporting guidelines were used in 72% of reviews. The quality of included studies was assessed in 80% of reviews. Only 12 reviews assessed the overall quality of evidence (50% of them using GRADE). Meta-analysis was conducted in 65% of reviews; only 1% used Bayesian methods. Random effect meta-analysis was used in 94% of reviews; among them, 75% did not report the variance estimator used. Among the reviews with meta-analysis, 70% did not report how data was transformed; 59% percent conducted subgroup analysis, 38% conducted meta-regression and 2% estimated prediction interval; I2 was estimated in 95% of analysis. Publication bias was examined in 48%. The most common software used was STATA (55%).Conclusions Our results indicate that there are significant inconsistencies regarding how these reviews are conducted. Many of these differences arose in the assessment of methodological quality and the formal synthesis of comparable data. This variability indicates the need for clearer reporting standards and consensus on methodological guidance for systematic reviews of prevalence data.
COX5A over-expression protects cortical neurons from hypoxic ischemic injury in neonatal rats associated with TPI up-regulation
Authors: Jiang Ya , Bai Xue , Zhao Qiong , Hawwas Mohammed AL , Jin Yuan , Zou Yu , Hu Yue , Li Ting-Ting , Liu Lin-Yi , Zhang Ying , Liu Qing , Yang Hao , Wang Tinghua , Ma Jun , Liu Jia , Xiong Liu-Lin 
Published: January 2020

Abstract
Background: Neonatal hypoxic-ischemic encephalopathy (HIE) is a destructive condition that constitutes a main cause of death in newborns. However, the underlying molecular mechanisms in brain damage are still not fully elucidated. Results: Here, we established hypoxic-ischemic (HI) injury and primary cortical neurons subjected to oxygen-glucose deprivation (OGD) to mimic HIE model in-vivo and in - vitro . Zea-longa scores, Triphenyte-trazoliumchloride (TTC) staining the Terminal Deoxynucleotidyl Transferased Utp Nick End Labeling (tunel) and immunofluorescent staining were used to detect the neurological injuries after HI. Then the expression of Cytochrome c oxidase subunit 5a (COX5A) was determined by immunohistochemistry, western blotting (WB) and quantitative real time Polymerase Chain Reaction (qRT-PCR) techniques. Moreover, HSV-mediated COX5A over-expression virus was administrated in - vitro to explore the role of COX5A in OGD neurons. Subsequently, the underlying mechanism was predicted by GeneMANIA and confirmed by WB and qRT-PCR. The results showed that HI induced a severe behavioral dysfunction, brain infarction, and cell apoptosis as well as obvious neuron loss in neonatal rats, in corresponding to the decrease on the expression of COX5A in both sides of the brain . What’s more, COX5A over-expression significantly promoted the neuron survival, reduced the apoptosis rate, and markedly increased the neurites length after OGD. Moreover, Triosephosephate isomerase (TPI) was predicted as physical interactions with COX5A, and COX5A over-expression largely increased the expressional level of TPI. Conclusions: Together, these data suggest that COX5A plays an important role in promoting neurological recovery after HI, and this process is related to TPI up-regulation.
NFKB1/NR3C1-MAPK4 axis regulates the pathology of acute lung injury
Authors: Mao Ling , Zhou Ya , Hu Lin , Liu Shiming , Zhao Juanjuan , Guo Mengmeng , Chen Chao , He Zhixu , Xu Lin 
Published: January 2020

Abstract
BackgroundAcute lung injury (ALI) is a serious disease with highly morbidity and mortality that causes serious health problems worldwide. MAPK4, a member of atypical MAPK family, has been implicated in the development of cancer. Herein, the current study aimed to investigate the possible role of MAPK4 in the pathology of ALI to identify potential candidates for ALI therapy.MethodsMurine ALI model was established in WT or MAPK4-/- mice and the expressions of MAPK4 were measured. The survival ratio of ALI model mice was observed. Moreover, the changes of pathologic injury and infiltration of inflammatory cells, as well as the related signaling pathways, in lung tissues were analyzed. Furthermore, the possible molecular mechanism of MAPK4 expression in ALI was analyzed by massARRAY and EMSA assay. Finally, the effect of MAPK4 silencing using shRNA interference on the pathology of ALI was identified.ResultsData showed that MAPK4 was up-regulated in lung tissues in LPS-induced murine ALI model. Importantly, MAPK4 deficiency mice exhibited prolonged survival time after LPS challenge, accompanied by alleviated inflammatory injury in lung tissues characterized with reduced production of pro-inflammatory cytokines, infiltration of immune cells and altered transduction of related signaling pathways. Besides, massARRAY results showed no aberrant change in CpG methylation levels between control and ALI mice. Bioinformatics analysis and EMSA assay showed that transcriptional factor NFKB1 and NR3C1 could negatively regulate the expression of MAKP4. Finally, MAPK4-shRNA treatment could ameliorate the pathology of lung tissues and prolong the survival time of mice after LPS challenge.ConclusionsOur data demonstrated that MAPK4, orchestrated by NFKB1 and NR3C1, could regulate the pathology of ALI, indicating that MAPK4 might be a new therapeutic target for ALI treatment.
Barriers and opportunities for management of shared sanitation facilities in low-income settlements in Kenya
Authors: Simiyu Sheillah , Kweyu Raphael M. , Antwi-Agyei Prince , Adjei Kwaku A. 
Published: January 2020

Abstract
Background Sharing of sanitation is commonly being practiced in low income areas in Sub Saharan Africa. However, the JMP categorizes shared sanitation as a limited sanitation service due to concerns of cleanliness and safety. The shared facilities are often the only option available for most of the residents in low income settlements, and improving the management is key to reducing open defecation and risk of disease. This study sought to investigate barriers and opportunities for improved management of shared sanitation in low income settlements of Kisumu in Kenya.Methods Thirty nine In-depth interviews and 11 Focus group discussions were held with residents, including tenants and landlords. Analysis followed a thematic approach to define the problem, specify the target behaviour and define what needs to change.Results Pit latrines were commonly shared among landlords and tenants. Shared sanitation facilities were unclean due to poor use and lack of cleaning. As respondents specified attributes of clean and useable shared toilets, they also identified behavioural, physical, social and motivational opportunities for improvement, and the key stakeholders that should be involved in shared sanitation interventions. Social opportunities such as clear cleaning plans, communication, and problem solving mechanisms between landlords and tenants were most commonly reported.Conclusion The results highlight the need to focus on social aspects for improvement of shared sanitation management in low income settlements. Through a social approach, shared sanitation facilities can be managed appropriately to afford the millions of low income dwellers an opportunity to access sanitation. This study provides further evidence on approaches for improved management of shared sanitation facilities in line with the JMP’s recommendation for well managed shared facilities.
The development of a questionnaire to assess leisure time screen-based media use and its proximal correlates in children (SCREENS-Q)
Authors: Klakk Heidi , Wester Christian Tolstrup , Olesen Line Grønholt , Rasmussen Martin Gillies , Kristensen Peter Lund , Pedersen Jesper , Grøntved Anders 
Published: January 2020

Abstract
Background: The screen-media landscape has changed drastically during the last decade with wide-scale ownership and use of new portable touchscreen-based devices plausibly causing changes in the volume of screen media use and the way children and young people entertain themselves and communicate with friends and family members. This rapid development is not sufficiently mirrored in available tools for measuring children’s screen media use. The aim of this study was to develop and evaluate a parent-reported standardized questionnaire to assess 6-10-year old children’s multiple screen media use and habits, their screen media environment, and its plausible proximal correlates based on a suggested socio-ecological model.
Methods: An iterative process was conducted developing the SCREENS questionnaire. Informed by the literature, media experts and end-users, a conceptual framework was made to guide the development of the questionnaire. Parents and media experts evaluated face and content validity. Pilot and field testing in the target group was conducted to assess test-retest reliability using Kappa statistics and intraclass correlation coefficients (ICC). Construct validity of relevant items was assessed using pairwise non-parametric correlations (Spearman’s). The SCREENS questionnaire is based on a multidimensional and formative model.
Results: The SCREENS questionnaire covers six domains validated to be important factors of screen media use in children and comprises 19 questions and 92 items. Test-retest reliability (n=37 parents) for continuous variables was moderate to substantial with ICC’s ranging from 0.67 to 0.90. For relevant nominal and ordinal data, kappa values were all above 0.50 with more than 80 percent of the values above 0.61 indicating good test-retest reliability. Internal consistency between two different time use variables (from n=243) showed good correlations with rho ranging from 0.59 to 0.66. Response-time was within 15 min for all participants.
Conclusions: SCREENS-Q is a comprehensive tool to assess children’s screen media habits, the screen media environment and possible related correlates. It is a feasible questionnaire with multiple validated constructs and moderate to substantial test-retest reliability of all evaluated items. The SCREENS-Q is a promising tool to investigate children screen media use.
Keywords: screen-media use, children, questionnaire, correlates