bluuebunny/crossref_metadata_2025_split huggingface dataset:
miRNA-126-3p carried by human umbilical cord mesenchymal stem cell enhances endothelial function through exosome-mediated mechanisms in vitro and attenuates vein graft neointimal formation in vivo
Authors: Qu Qingxi , Wang Limei , Bing Weidong , Bi Yanwen , Zhang Chunmei , Jing Xuanxuan , Liu Linghong 
Published: November 2020

Abstract

Background
The aim of this study was to determine whether the combination of MSC implantation with miRNA-126-3p overexpression would further improve the surgical results after vein grafting.

Methods
human umbilical cord MSCs (hucMSCs) and human umbilical vein endothelial cells (HUVECs) were isolated from human umbilical cords and characterized by a series of experiments. Lentivirus vector encoding miRNA-126-3p was transfected into hucMSCs and verified by PCR. We analyzed the miRNA-126-3p-hucMSC function in vascular endothelial cells by using a series of co-culture experiments. miRNA-126-3p-hucMSCs-exosomes were separated from cell culture supernatants and identified by WB and TEM. We validated the role of miRNA-126-3p-hucMSCs-exosomes on HUVECs proliferative and migratory and angiogenic activities by using a series of function experiments. We further performed co-culture experiments to detect downstream target genes and signaling pathways of miRNA-126-3p-hucMSCs in HUVECs. We established a rat vein grafting model, CM-Dil-labeled hucMSCs were injected intravenously into rats, and the transplanted cells homing to the vein grafts were detected by fluorescent microscopy. We performed historical and immunohistochemical experiments to exam miRNA-126-3p-hucMSC transplantation on vein graft neointimal formation and reendothelialization in vitro.

Results
We successfully isolated and identified primary hucMSCs and HUVECs. Primary hucMSCs were transfected with lentiviral vectors carrying miRNA-126-3p at a MOI 75. Co-culture studies indicated that overexpression of miRNA-126-3p in hucMSCs enhanced HUVECs proliferation, migration, and tube formation in vivo. We successfully separated hucMSCs-exosomes and found that miRNA-126-3p-hucMSCs-exosomes can strengthen the proliferative, migratory, and tube formation capacities of HUVECs. Further PCR and WB analysis indicated that, SPRED-1/PIK3R2/AKT/ERK1/2 pathways are involved in this process. In the rat vein arterialization model, reendothelialization analysis showed that transplantation with hucMSCs modified with miRNA-126-3p had a higher reendothelialization of the vein grafts. The subsequent historical and immunohistochemical examination revealed that delivery with miRNA-126-3p overexpressed hucMSCs significantly reduced vein graft intimal hyperplasia in rats.

Conclusion
These results suggest hucMSC-based miRNA-126-3p gene therapy may be a novel option for the treatment of vein graft disease after CABG.
Identification of stress-responsive transcription factors with protein-bound Escherichia coli genomic DNA libraries
Authors: Li Xianqiang , Jiang Xin , Xu Meiying , Fang Yun , Wang Yan , Sun Guoping , Guo Jun 
Published: November 2020

AbstractBacteria promoters along with operators are crucial elements in the control of gene expression in microbes in response to environmental stress changes. A genome-wide promoter DNA regulatory library is in demand to be developed for a microbe reporter method to monitor the existence of any given environmental stress substance. In this study, we utilizedEscherichia coli(E. coli) as a model system for the preparation of both cell lysates and genomic DNA fragments. Through enriching protein-bound DNA fragments to construct luciferase reporter libraries, we found that, of 280 clones collected and sequenced, 131 clones contained either the promoter-35 and -10 conservative sequences and/or an operator transcription factor binding sites (TFBS) region. To demonstrate the functionality of the identified clones, five of 131 clones containing LexA binding sequence have been demonstrated to be induced in response to mitomycin C treatment. To evaluate our libraries as a functional screening library, 80 randomly picked up clones were cultured and treated with and without MMC, where two clones were shown to have greater than twofold induction. In addition, two arsenite-responsive clones were identified from 90 clones, one having the well-known ArsR and another having the osmotically inducible lipoprotein (OsmE1). The newly discoveredosmE1has been quantitatively validated to be induced by arsenite treatment with real-time PCR in a dose response and time course manner. This enriching protein-bound DNA luciferase reporter libraries and functional screening facilitate the identification of stress-responsive transcriptional factors in microbes. We developed functional libraries containingE. coligenomic-wide protein-bound DNA as enhancers/operators to regulate downstream luciferase in response to stress.
Effects of capillary refill time-vs. lactate-targeted fluid resuscitation on regional, microcirculatory and hypoxia-related perfusion parameters in septic shock: a randomized controlled trial
Authors: Castro Ricardo , Kattan Eduardo , Ferri Giorgio , Pairumani Ronald , Valenzuela Emilio Daniel , Alegría Leyla , Oviedo Vanessa , Pavez Nicolás , Soto Dagoberto , Vera Magdalena , Santis César , Astudillo Brusela , Cid María Alicia , Bravo Sebastian , Ospina-Tascón Gustavo , Bakker Jan , Hernández Glenn 
Published: November 2020

Abstract

Background
Persistent hyperlactatemia has been considered as a signal of tissue hypoperfusion in septic shock patients, but multiple non-hypoperfusion-related pathogenic mechanisms could be involved. Therefore, pursuing lactate normalization may lead to the risk of fluid overload. Peripheral perfusion, assessed by the capillary refill time (CRT), could be an effective alternative resuscitation target as recently demonstrated by the ANDROMEDA-SHOCK trial. We designed the present randomized controlled trial to address the impact of a CRT-targeted (CRT-T) vs. a lactate-targeted (LAC-T) fluid resuscitation strategy on fluid balances within 24 h of septic shock diagnosis. In addition, we compared the effects of both strategies on organ dysfunction, regional and microcirculatory flow, and tissue hypoxia surrogates.

Results
Forty-two fluid-responsive septic shock patients were randomized into CRT-T or LAC-T groups. Fluids were administered until target achievement during the 6 h intervention period, or until safety criteria were met. CRT-T was aimed at CRT normalization (≤ 3 s), whereas in LAC-T the goal was lactate normalization (≤ 2 mmol/L) or a 20% decrease every 2 h. Multimodal perfusion monitoring included sublingual microcirculatory assessment; plasma-disappearance rate of indocyanine green; muscle oxygen saturation; central venous-arterial pCO2 gradient/ arterial-venous O2 content difference ratio; and lactate/pyruvate ratio. There was no difference between CRT-T vs. LAC-T in 6 h-fluid boluses (875 [375–2625] vs. 1500 [1000–2000], p = 0.3), or balances (982[249–2833] vs. 15,800 [740–6587, p = 0.2]). CRT-T was associated with a higher achievement of the predefined perfusion target (62 vs. 24, p = 0.03). No significant differences in perfusion-related variables or hypoxia surrogates were observed.

Conclusions
CRT-targeted fluid resuscitation was not superior to a lactate-targeted one on fluid administration or balances. However, it was associated with comparable effects on regional and microcirculatory flow parameters and hypoxia surrogates, and a faster achievement of the predefined resuscitation target. Our data suggest that stopping fluids in patients with CRT ≤ 3 s appears as safe in terms of tissue perfusion.
Clinical Trials: ClinicalTrials.gov Identifier: NCT03762005 (Retrospectively registered on December 3rd 2018)
Research on mechanical vibration monitoring based on wireless sensor network and sparse Bayes
Authors: Lei Xinjun , Wu Yunxin 
Published: November 2020

Abstract
Mechanical vibration monitoring for rotating mechanical equipment can improve the safety and reliability of the equipment. The traditional wired monitoring technology faces problems such as high-frequency signal pickup and high-precision data collection. Therefore, this paper proposes optimization techniques for mechanical vibration monitoring and signal processing based on wireless sensor networks. First, the hardware design uses high-performance STM32 as the control center and Si4463 as the wireless transceiver core. The monitoring node uses a high-precision MEMS acceleration sensor with a 16-bit resolution ADC acquisition chip to achieve high-frequency, high-precision acquisition of vibration signals. Then, the bearing vibration signal optimization method is studied, and the sparse Bayes algorithm is proposed as a compressed sensing reconstruction algorithm. Finally, the difference in reconstruction accuracy between this method and the traditional reconstruction algorithm is compared through experiments and the effect of this method on the reconstruction performance is analyzed when different parameters are selected.
Fixed point results for Geraghty quasi-contraction type mappings in dislocated quasi-metric spaces
Authors: Umudu Joy C. , Olaleru Johnson O. , Mogbademu Adesanmi A. 
Published: November 2020

Abstract
In this paper, fixed point results for a newly introduced Geraghty quasi-contraction type mappings are proved in more general metric spaces called T-orbitally complete dislocated quasi-metric spaces. Geraghty quasi-contraction type mappings generalize, among others, Ciric’s quasi-contraction mappings and other Geraghty quasi-contractive type mappings in the literature. Fixed point results are obtained without imposing a continuity condition on the mapping, thereby further generalizing some other related work in the literature. An example is given to show the validity of results obtained.
Sustainability of residential environmental interventions and health outcomes in the elderly
Authors: Turcotte David A. , Woskie Susan , Gore Rebecca , Chaves Emily , Adejumo Kelechi L. , You Kim-Judy 
Published: November 2020

Abstract

Background
Research has documented that housing conditions can negatively impact the health of residents. Asthma has many known indoor environmental triggers including dust, pests, smoke and mold, as evidenced by the 25 million people in the U.S. population who have asthma. The paper describes a follow-up study involving elder adults with asthma who participated in a multifaceted home educational and environmental intervention shown to produce significant health benefits. On average the time between the end of the prior intervention study and the follow-up was 2.3 years. The objective of this study was to evaluate whether improvements in environmental conditions and health outcomes resulting from the original Older Adult Study (OAS, multifaceted educational and environmental interventions) would be maintained or decline over time for these low income seniors with asthma.

Methods
Health assessment included data on respiratory health outcomes included the Saint George’s Respiratory Questionnaire (SGRQ) and Asthma Control Test from the original Older Adult Study (OAS) and this follow-up Older Adult Study (OAFS) along with health care utilization data. Environmental assessments included evaluation of asthma trigger activities (ATAs) and exposures before and after the original healthy homes intervention (questionnaire, home survey) and at this follow-up. Assessments were conducted in English, Khmer and Spanish.

Results
At assessment in the Older Adult Follow-up Study (OAFS), the older adults maintained some of the health improvements gained during the OAS when compared to the OAS pre-intervention baseline. However, health outcomes declined from the OAS final assessment to the OAFS (only the SGRQ Impact scores were significantly different).

Conclusion
These findings suggest that further study with a larger population is needed to determine if the significant health outcome improvements from multifaceted home educational and environmental interventions (OAS) could be more strongly maintained by providing additional follow-up “booster” interventions to this older adult population with asthma.
A new scoring system for predicting extent of resection in medial sphenoid wing meningiomas based on three-dimensional multimodality fusion imaging
Authors: Wang Zilan , Liang Xiaolong , Yang Yanbo , Gao Bixi , Wang Ling , You Wanchun , Chen Zhouqing , Wang Zhong 
Published: November 2020

Abstract

Background
Three-dimensional (3D) fusion imaging has been proved to be a promising neurosurgical tool for presurgical evaluation of tumor removal. We aim to develop a scoring system based on this new tool to predict the resection grade of medial sphenoid wing meningiomas (mSWM) intuitively.

Methods
We included 46 patients treated for mSWM from 2014 to 2019 to evaluate their tumors’ location, volume, cavernous sinus involvement, vascular encasement, and bone invasion by 3D multimodality fusion imaging. A scoring system based on the significant parameters detected by statistical analysis was created and evaluated.

Results
The tumor volumes ranged from 0.8 cm3 to 171.9 cm3. A total of 39 (84.8%) patients had arterial involvement. Cavernous sinus (CS) involvement was observed in 23 patients (50.0%) and bone invasion was noted in 10 patients (21.7%). Simpson I resection was achieved in 10 patients (21.7%) and Simpson II resection was achieved in 17 patients (37.0%). Fifteen patients (32.6%) underwent Simpson III resection and 4 patients (8.7%) underwent Simpson IV resections. A scoring system was created. The score ranged from 1 to 10 and the mean score of our patients was 5.3 ± 2.8. Strong positive monotonic correlation existed between the score and resection grade (Rs = 0.772, P < 0.001). The scoring system had good predictive capacity with an accuracy of 69.60%.

Conclusions
We described a scoring system that enabled neurosurgeons to predict extent of resection and outcomes for mSWM preoperatively with 3D multimodality fusion imaging.

Trial registration
Retrospectively registered
Validity of endocan as a biomarker in systemic sclerosis: relation to pathogenesis and disease activity
Authors: Mahgoub Marwa Yahia , Fouda Ali Ibrahim , Elshambaky Ahmed Yousef , Elgazzar Walaa Bayoumie , Shalaby Sara Abdellatif 
Published: November 2020

Abstract
Background
Systemic sclerosis (SSc), an autoimmune disease, has endothelial dysfunction and tissue fibrosis. The lack of its specific activity markers urges the research. Endocan is an immunomodulatory protein associated with endothelial dysfunction. We aimed to measure of the endocan level in systemic sclerosis (SSc) patients’ sera to test its relation to disease activity and treatment responses. Also, to evaluate its specificity by comparing these data with Behçet’s disease (BD) and rheumatoid arthritis (RA) patients’ data.

Results
SSc and RA patients were in activity but in the BD group; eight were active and seven were inactive. The endocan level was higher in the three groups in comparison to the controls without statistically significant differences (p value RA 0.697, SSc 0.063, and BD 0.196). A statistically significant difference in endocan levels between SSc patients with and without vascular manifestations (pulmonary hypertension and finger pitting ulcers, p < 0.0001, p = 0.0097, respectively). There were no significant correlations between endocan level and the erythrocyte sedimentation rate and C-reactive protein (positive rheumatoid factor for RA) in the three groups (p > 0.05). There were no significant correlations between endocan level and activity scores in the three groups (p > 0.05). The cut-off value of endocan was 1.3 ng/ml.

Conclusion
Endocan is a non-specific marker for the vascular pathogenesis in systemic sclerosis but not a good predictor for its activity.
Preparation of Cu@Ag Core-Shell Nanoparticles by NaBH4 Combined with NaH2PO2 as Reductants
Authors: Ma Yuling , Wang Lin , Ye Qin , Qin Haihang , Fu Qiubo 
Published: November 2020

Abstract
In this work, sodium borohydride (NaBH4) and sodium hypophosphite (NaH2PO2) were simultaneously used as reductants to prepare Cu@Ag core-shell nanoparticles with a particle size of 300–500 nm. Results proved that Cu@Ag particles with different particle sizes could be obtained by changing the molar ratio of NaBH4 to NaH2PO2 and reaction temperature. The characterization of prepared samples demonstrated that the decoupling effect of NaBH4 and NaH2PO2 could be a promising method for modifying the core-shell particle size.
Williams–Campbell syndrome: an unusual presentation in an adult patient
Authors: Rohilla Mili , Previgliano Carlos , Geimadi Atefeh , Sangster Guillermo 
Published: November 2020

Objective: Williams–Campbell syndrome (WCS) is a rare congenital disorder, which leads to bronchiectasis affecting fourth to sixth order of bronchial divisions. Symptoms include cough, sputum, wheeze and recurrent pulmonary infections, classically seen in the paediatric age group with selective bronchiectasis of the mid-order bronchioles. The literature describing diagnosis of Williams–Campbell syndrome in adult population is very sparse. Methods: This report presents a 62-year-old female with cough, fever, dyspnea and generalized body ache. She has had multiple admissions to the hospital since her childhood due to recurrent lower respiratory tract infections. Imaging findings demonstrated multiple cystic thin walled airways, compatible with bronchiectatic changes in the upper, middle and lower lobes bilaterally, bronchial wall thickening with air-fluid levels prominent in the fifth and sixth generation bronchial divisions, with normal calibre trachea and central bronchi. These radiological findings are consistent with diagnosis of Williams–Campbell syndrome, which was diagnosed after ruling out the other common causes of bronchiectasis. Conclusion: Williams–Campbell syndrome is a rare congenital cystic lung disease, the diagnosis of which is made by exclusion of common causes of bronchiectasis such as cystic fibrosis, allergic bronchopulmonary aspergillosis, tuberculosis, dyskinetic cilia syndrome and alpha-1 antitrypsin deficiency. Whenever the clinical picture is consistent with bronchiectasis, especially involving the mid-order bronchioles and recurrent pulmonary infections, it is wise to include WCS in the list of differential diagnoses, even in the adult population.
Factors associated with underutilisation of antenatal care services in Limpopo, South Africa
Authors: Mulondo Seani Adrinah 
Published: November 2020

Background The aim of this study was to explore and describe factors associated with underutilisation of antenatal care services. In South Africa, maternal and child healthcare services are provided free of charge. Despite this, utilisation of antenatal care services remains a major public health challenge. Literature affirms that 96% of pregnant women present at antenatal care services only after 12 weeks of pregnancy, and this is influenced by several factors. Women's attitude plays an integral role. Methods This study used a qualitative, exploratory, descriptive design. Purposive and convenient sampling methods were used to select 10 hospitals and 83 pregnant women, respectively. Ten focus group discussions were conducted to collect data. Trustworthiness was ensured by means of Guba's model and data were analysed, qualitatively, using Tesch's eight steps. Results The majority of the pregnant women (83%) were from rural areas, had unplanned pregnancies, had five pregnancies/children or more, factors that are associated with underutilisation of antenatal care services. Cultural practices and beliefs regarding early presentation to antenatal care services and its effect on pregnancies were also found to be associated with underutilisation. Conclusions Some pregnant women do not consider antenatal care services as essential and choose to present late, despite the knowledge that monitoring of maternal health during pregnancy by midwives could prevent maternal and neonatal morbidity, while improving maternal and neonatal health and wellness. Recommendations include the provision of information and education materials to women, as well as health education, to sensitise and increase women's awareness of the urgency in utilising antenatal care services.
How useful are virtual fracture clinics?
Authors: Khan Shehzaad A. , Asokan Ajay , Handford Charles , Logan Peter , Moores Thomas 
Published: November 2020

Background Due to the overwhelming demand for trauma services, resulting from increasing emergency department attendances over the past decade, virtual fracture clinics (VFCs) have become the fashion to keep up with the demand and help comply with the BOA Standards for Trauma and Orthopaedics (BOAST) guidelines. In this article, we perform a systematic review asking, “How useful are VFCs?”, and what injuries and conditions can be treated safely and effectively, to help decrease patient face to face consultations. Our primary outcomes were patient satisfaction, clinical efficiency and cost analysis, and clinical outcomes. Methods We performed a systematic literature search of all papers pertaining to VFCs, using the search engines PubMed, MEDLINE, and the Cochrane Database, according to the Preferred Reporting Items for Systematic review and Meta-Analysis (PRISMA) checklist. Searches were carried out and screened by two authors, with final study eligibility confirmed by the senior author. Results In total, 21 records were relevant to our research question. Six orthopaedic injuries were identified as suitable for VFC review, with a further four discussed in detail. A reduction of face to face appointments of up to 50% was reported with greater compliance to BOAST guidelines (46.4%) and cost saving (up to £212,000). Conclusions This systematic review demonstrates that the VFC model can help deliver a safe, more cost-effective, and more efficient arm of the trauma service to patients. Cite this article: Bone Joint Open 2020;1-11:683–690.