bluuebunny/crossref_metadata_2025_split huggingface dataset:
Contraceptive efficacy of sperm agglutinating factor from Staphylococcus warneri, isolated from the cervix of a woman with inexplicable infertility
Authors: Pant Neeraj Chandra , Singh Ravinder , Gupta Vijaya , Chauhan Aditi , Mavuduru Ravimohan , Prabha Vijay , Sharma Prince 
Published: October 2019

AbstractBackgroundVoluntary control of fertility is of paramount importance to the modern society. But since the contraceptive methods available for women have their limitations such as urinary tract infections, allergies, cervical erosion and discomfort, a desperate need exists to develop safe methods. Vaginal contraceptives may be the answer to this problem, as these are the oldest ways of fertility regulation, practiced over the centuries. With minimal systemic involvement, these are also the safest. Natural substances blocking or impairing the sperm motility offer as valuable non-cytotoxic vaginal contraceptives. Antimicrobial peptides (AMPs) isolated from plants, animals and microorganisms are known to possess sperm immobilizing and spermicidal properties. Following this, in the quest for alternative means, we have cloned, over expressed and purified the recombinant sperm agglutinating factor (SAF) fromStaphylococcus warneri,isolated from the cervix of a woman with unexplained infertility.MethodsGenomic library ofStaphylococcus warneriwas generated inEscherichia coliusing pSMART vector and screened for sperm agglutinating factor (SAF). The insert in sperm agglutinating transformant was sequenced and was found to express ribonucleotide-diphosphate reductase-α sub unit. The ORF was sub-cloned in pET28a vector, expressed and purified. The effect of rSAF on motility, viability, morphology, Mg++-dependent ATPase activity and acrosome status of human sperms was analyzed in vitro and contraceptive efficacy was evaluated in vivo in female BALB/c mice.ResultsThe 80 kDa rSAF showed complete sperm agglutination,inhibited its Mg2+-ATPase activity, caused premature sperm acrosomal loss in vitro and mimicked the pattern in vivo showing 100% contraception in BALB/c mice resulting in prevention of pregnancy. The FITC labeled SAF was found to bind the entire surface of spermatozoa. Vaginal application and oral administration of rSAF to mice for 14 successive days did not demonstrate any significant change in vaginal cell morphology, organ weight and tissue histology of reproductive and non-reproductive organs and had no negative impact in the dermal and penile irritation tests.ConclusionThe Sperm Agglutinating Factor fromStaphylococcus warneri,natural microflora of human cervix, showed extensive potential to be employed as a safe vaginal contraceptive.
Increased expression of TET3 predicts unfavorable prognosis in patients with ovarian cancer-a bioinformatics integrative analysis
Authors: Cao Tiefeng , Pan Wenwei , Sun Xiaoli , Shen Huimin 
Published: October 2019

Abstract
Ovarian carcinoma is a lethal gynecological malignancy. Women with ovarian cancer (OC) are highly recurrent and typically diagnosed at late stage. Ten-eleven translocation protein 3 (TET3) belongs to the family of ten-eleven translocations (TETs) which induce DNA demethylation and gene regulation in epigenetic level by converting 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC). Previous studies indicated that TET3 is overexpressed in ovarian cancer tissues. However, the clinic-pathological functions and prognostic values of TET3 remain unclear. Here we performed an integrative study to identify the role of TET3 by bioinformatics analysis. The TET3 expression in ovarian cancer was assessed with Oncomine database, and validated with TCGA and GTEx database. The correlation of TET3 gene alteration and clinic-pathological functions was addressed by integrative analysis of GEO datasets. Then we showed mainly TET3 gain and diploid but less deletion in ovarian cancer by copy number alteration (CNA) or mutation analysis with cBioPortal. Furthermore, by using Kaplan-Meier plotter (K-M plotter), we evaluated that high TET3 level was associated with poor survival in ovarian cancer patients, which was validated with analysis by PrognoScan database and gene differential analyses with TCGA and GTEx. This is the first study demonstrated that elevated expression of TET3 is associated with poor clinic-pathological functions, poor prognosis, wherein TET3, which presents epigenetic changes or methylation changes, might be served as a diagnostic marker or therapeutic target for ovarian cancer.
Papillary squamotransitional cell carcinoma of the uterine cervix: a case report and review of the literature
Authors: Gitas Georgios , Ertan Kubilay , Rody Achim , Baum Sascha , Tsolakidis Dimitrios , Alkatout Ibrahim 
Published: October 2019

Abstract

Background
Papillary squamotransitional cell carcinoma of the uterine cervix is a rare neoplasm, a subtype of transitional cervical carcinoma that appears to be a variation of squamous cervical carcinoma. It has a disposition toward metastasis at an advanced stage and local recurrence. Owing to the difficulty of illustrating the invasion histologically, misdiagnosis is likely to affect the patient’s prognosis.

Case presentation
We present a case report of an 81-year-old Caucasian patient with squamotransitional cell carcinoma with unusual clinical behavior that was primarily thought to be ovarian cancer. According to the clinical examination and radiologic imaging, the patient had no vaginal bleeding and a normal cervix. Nevertheless, the tumor was already metastasized at the retroperitoneal tissue and at the right ovary. Computed tomography-guided biopsy of the right adnexa gave no further clarification. Although the tumor resembled urothelial cancer, this diagnosis was dismissed because of the results of immunohistochemistry analysis with CK7+, CK5+, and CK20−. Because of the differential diagnosis of ovarian cancer, we decided in favor of an exploratory surgical approach. Hysterectomy with bilateral adnexectomy, extensive retroperitoneal tumor debulking, and infragastric omentectomy was performed by laparotomy. Histopathology revealed a squamotransitional cervical cancer as the primary tumor with a tumor stage of pT3b, pN1 (1/2), V0, RX, G2, corresponding to International Federation of Gynecology and Obstetrics stage IIIB.

Conclusions
As far as we are aware, this is the first report of papillary squamotransitional cell carcinoma of the uterine cervix metastatic to the ovary without vaginal bleeding and with a clinically and radiologically unsuspicious cervix. Physicians should always contemplate papillary squamotransitional cell carcinoma of the uterine cervix in unclear cases with ovarian metastasis, especially if the histology indicates a transitional cancer (CK7+ and CK20−), before proceeding with treatment. More cases are needed to illuminate the clinical characteristics and categorization of papillary squamotransitional cell carcinoma of the uterine cervix.
Pilot randomized controlled trial of a complex intervention for diabetes self-management supported by volunteers, technology, and interprofessional primary health care teams
Authors: Agarwal Gina , Gaber Jessica , Richardson Julie , Mangin Dee , Ploeg Jenny , Valaitis Ruta , Reid Graham J. , Lamarche Larkin , Parascandalo Fiona , Javadi Dena , O’Reilly Daria , Dolovich Lisa 
Published: October 2019

Abstract

Background
Most health care for people with diabetes occurs in family practice, yet balancing the time and resources to help these patients can be difficult. An intervention empowering patients, leveraging community resources, and assisting self-management could benefit patients and providers. Thus, the feasibility and potential for effectiveness of “Health Teams Advancing Patient Experience, Strengthening Quality through Health Connectors for Diabetes Management” (Health TAPESTRY-HC-DM) as an approach supporting diabetes self-management was explored to inform development of a future large-scale trial.

Methods
Four-month pilot randomized controlled trial (RCT), sequential explanatory qualitative component. Participants—patients of an interprofessional primary care team—were over age 18 years, diagnosed with diabetes and hypertension, and had Internet access and one of the following: uncontrolled HbA1c, recent diabetes diagnosis, end-stage/secondary organ damage, or provider referral. The Health TAPESTRY-HC-DM intervention focused on patient health goals/needs, integrating community volunteers, eHealth technologies, interprofessional primary care teams, and system navigation. Pilot outcomes included process measures (recruitment, retention, program participation), perceived program feasibility, benefits and areas for improvement, and risks or safety issues. The primary trial outcome was self-efficacy for managing diabetes. There were a number of secondary trial outcomes.

Results
Of 425 eligible patients invited, 50 signed consent (11.8%) and 35 completed the program (15 intervention, 20 control). Volunteers (n = 20) met 28 clients in 234 client encounters (home visits, phone calls, electronic messages); 27 reports were sent to the interprofessional team. At 4 months, controlling for baseline, most outcomes were better in the intervention compared to control group; physical activity notably better. The most common goal domains set were physical activity, diet/nutrition, and social connection. Clients felt the biggest impact was motivation toward goal achievement. They struggled with some of the technologies. Several participants perceived that the program was not a good fit, mostly those that felt they were already well-managing their diabetes.

Conclusions
Health TAPESTRY-HC-DM was feasible; a large-scale randomized controlled trial seems possible. However, further attention needs to be paid to improving recruitment and retention. The intervention was well received, though was a better fit for some participants than others.

Trial registration
ClinicalTrials.gov, NCT02715791. Registered 22 March 2016—retrospectively registered.
Preface
Authors: Karm Mari , Poom-Valickis Katrin 
Published: October 2019

Eesti Haridusteaduste Ajakirja erinumbris tutvustatakse võimalusi rakendada tegevusuuringut eesmärgiga arendada õpetamist. Kuna hariduse kvaliteet sõltub õpetamise kvaliteedist, võimaldab tegevusuuring süstemaatiliselt analüüsida enda õpetamistavasid. Analüüsi tulemusena saab õpetamises teha muudatusi ja seeläbi eesmärgipäraselt toetada õppijate õppimist. Erinumbri artiklites tutvustatavad tegevusuuringud on tehtud eri õpikeskkondades (kool, kutsekool, ülikool), otsides lahendusi eritahulistele probleemidele. Raamatututvustus on aga pühendatud John Hattie ja Gregory Yatesi raamatule "Nähtav õppimine ja teadus sellest, kuidas me õpime".
More efficient engineering education through supporting implicit ability beliefs
Authors: Petjärv Britt , Aus Kati , Arro Grete 
Published: October 2019

Tööturustatistika kohaselt on inseneride nappus Eestis suur probleem. Uurides kõrgkoolist väljalangemust, selgub paraku, et just tehnilist kõrgharidust omandavate üliõpilaste seas on väljalangemus kõige suurem. Uuringud näitavad, et vaimse võimekusega seotud juurdekasvuuskumuste toetamine parandab õppijate edasijõudmist matemaatikas ning et tehnikavaldkonna üliõpilaste implitsiitsed võimekususkumused mõjutavad kõrgkooliõpingutes edasijõudmist, sealhulgas väljalangemist. Siinses artiklis võetakse vaatluse alla Tallinna Tehnikakõrgkooli esimese õppeaasta üliõpilaste (N = 270) uskumused võimekuse muudetavuse kohta ning muud motivatsiooni ja õpipädevusega seotud tegurid. Uuringu eesmärk on hinnata, millised õppimisega seotud aspektid ennustavad edasijõudmist inseneriõppes. Samuti uuritakse, kas lühiajalise sekkumisega on võimalik muuta õppijate mõtteviisi juurdekasvuuskumuste suunas. Eksperimentaalsest sekkumisuuringust ühe õppekava alusel õppivate esmakursuslaste seas (N = 37) ilmneb, et sekkumisrühma üliõpilaste mõtteviisi muutus oli positiivne ehk jäävususkumused võimekuse muudetavuse kohta muutusid nõrgemaks. See osutab vajadusele toetada õppimisprotsessi puudutavaid arusaamu süsteemselt, õpetamismetoodikasse integreerituna ning kavandada õppejõudude õpetamis- ja õppimisalaste teadmiste arendamiseks asjakohaseid koolitusi.
Summary
Developing students’ academic literacies via Wikipedia-based tasks
Authors: Reinsalu Riina , Zupping Sirli , Voolaid Ene 
Published: October 2019

Üliõpilaste akadeemilise kirjaoskuse arendamine on üks ülikooliõpingutele seatud eesmärke, kuid selle täitmist võivad takistada mitmesugused tegurid, sh üli õpilaste vähene huvi kirjutamisülesande vastu. Üks võimalus suurendada üliõpilaste huvi on asendada traditsiooniline referaat või essee veebis vabalt kättesaadava vikiartikliga. Siinses uurimuses on seatud eesmärgiks välja selgitada vikiülesande võimalused toetada üliõpilaste akadeemilise kirjaoskuse arengut. Tartu Ülikooli väljendusõpetuse kursuste õppejõudude, õppeassistentide ja üliõpilastega tehtud fookusrühma intervjuudest ilmneb, et vikiartikli ülesande kaudu saab peale kirjutamisoskuse muu hulgas arendada üliõpilaste digi- ja infopädevust, uurimis- ja analüüsioskust. Ühtlasi on Vikipeediasse kui avalikku veebientsüklopeediasse kirjutamine tugev motivaator, mis sunnib üliõpilasi pingutama, kuid see võib osa üliõpilaste jaoks olla ka stressiallikas, sest nad tunnevad suurt vastutust loodud artikli eest.
Summary
Integration of the theory of vocational didactics with school practice: observations of vocational teacher students
Authors: Liivik Reeli , Rekkor Sirje , Sirk Meidi 
Published: October 2019

Kutseõppedidaktikas keskendutakse kutseõppe ja töömaailma sidususest lähtudes väljundipõhise õppe planeerimisele, rakendamisele ja hindamisele ning kutseõppedidaktikal on oluline koht kutseõpetajakoolituses ja igapäevatöös. Uurimustest ilmneb, et õpetajad ei mõtesta sageli kutseõppedidaktikat teoreetilise kontseptsioonina ning väljundipõhise kutseõppe põhimõtted ei ole kutsehariduses täielikult juurdunud. Artikli eesmärk on selgitada kutseõpetajakoolituse üliõpilaste oskust seostada omandatud kutseõppedidaktika teoreetilisi teadmisi koolitegelikkusega. Rakendatav kvalitatiivne temaatiline analüüs põhineb 12 üliõpilase praktika vaatlusülesandes esile toodud tähelepanekutel ja seostel kutseõppedidaktika teooriaga. Uurimistulemused näitavad, et üliõpilaste tähelepanekud kirjeldavad lihtsustatult kutseõppedidaktika teooria ilmnemist koolitegelikkuses ja vaatluste tõlgendused seostuvad tugevamalt vaatlusperioodil omandatud teoreetiliste teadmistega kutseõppedidaktika kohta.
Summary
Lecturer’s and assistant’s views on collaborative teaching at the university
Authors: Lõhmus Eleri , Narits Henry , Ugur Kadri 
Published: October 2019

Märksõna koostöö on kõrghariduse võlu- ja võtmesõna, mis aitab toime tulla piiratud ressursside ning kasvava konkurentsiga. Ühise õpetamise vorme on kõrgkoolides kasutusel palju, alates loengute jagamisest eri õppejõudude vahel kuni õpetamiseni võrdselt vastutavate ja panustavate partneritena dialoogi pidades. Artiklis kirjeldatakse ja analüüsitakse Eleri Lõhmuse ja Henry Naritsa koosõpetamise kogemust audiovisuaalse produktsiooni praktilisel kursusel Tartu Ülikooli ühiskonnateaduste instituudis 2018. aasta sügissemestril. Artikkel põhineb autoetnograafiliselt dokumenteeritud materjalil, mida Lõhmus ja Narits kogusid ühistöö käigus. Artikli kolmas autor tegi intervjuud üliõpilastega, et lisada autoetnograafilistele tähelepanekutele õppija vaatepunkt. Kogutud empiirilise materjali analüüs osutab, et koosõpetamine kõrgkoolis on efektiivne ja arendav kõigile osalistele, aga õppetöö õnnestumine sõltub planeerimis- ja refleksioonioskustest ning vajab praegusest rohkem formaalset tuge.
Summary
BAKTERI ENTOMOPATOGEN SEBAGAI AGEN BIOKONTROL TERHADAP LARVA Spodoptera litura (F.)
Authors: Zulfiana Deni , Krishanti Ni Putu Ratna Ayu , Wikantyoso Bramantyo , Zulfitri Apriwi 
Published: October 2019

Spodoptera litura (F.) (Lepidoptera: Noctuidae) is one of the agricultural pests that attacking almost all kinds of herbaceous plants, especiallyvegetables. Insect control using entomopathogenic bacteria is an alternative strategy that is effective and has a lower environmental impact than the use of synthetic insecticides. The purpose of this research was to explore entomopathogenic bacteria that have insecticidal activity against S. litura larvae at various stages of instars. The result showed that 25% of total number of isolated bacteria have potency as entomopathogenic bacteria. Isolate Staphylococcus sciuri strain BLSP-3 and isolate Serratia sp. strain BLSP-4 showed the highest larvicidal activity against the first and second instar larvae of S. litura 83% and 86%, respectively. The activity against on the third instar larvae however was only by 40%. However, the mortality caused by both isolates was lower than that of Bacillus thuringiensis (more than 90% mortality to the first and second instars and 80 % of the third instar larvae). It is suggested that both of isolates are potential to be developed further as a biocontrol agent to control S. litura population.
PENINGKATAN PERTUMBUHAN PADI VAR CIHERANG SETELAH DIINOKULASI DENGAN Azospirillum MUTAN MULTIFUNGSI PENAMBAT N2, PELARUT P DAN PENGHASIL FITOHORMON INDOLE ACETIC ACID (IAA).
Authors: Riyanti Eny Ida , Listanto Edy 
Published: October 2019

Modern agriculture rellies on the application of fertilizer to enhanched plant growth. Excessive application of chemical fertilizers in the long term will decrease soil fertility, therefore the use of biological fertilizers is expected to reduce the negative impact. The aim of this study was to determine the effect of multi-functional Azospirillum for N2-fixation, phosphate solubilizerÂ and producing phytohormone Indole Acetic Acid (IAA) on the growth of rice plants Ciherang in the pot experiment conducted in the BB Biogenâ€™s greenhouse. Two treatments were tested: types of inoculation (not inoculated, were inoculated with the wildtype AjB 6.4.1.2 and inoculated with mutant isolates AJM 3.7.1.14), and 4 levels of fertilizer application (not fertilized, a quarter dose of paddy fertilizer recommendations, a half dose of fertilizer rice in the rice fields, and the appropriate dose of fertilizer in paddy fields). Azospirillum used is wildtype isolate AjB 6.4.1.2 and mutant isolate AJM 3.7.1.14 isolated and and mutated in BB Biogen. Ciherang rice seed inoculated with Azospirillum on the cell density of 106 cells / ml at the different seedlings tub. After the age of 14 days after planting, the seedlings were transferred into pots with three plants per pot. Parameters measured were: plant height, number of tillers, number of panicles per clump, fresh weight and dry panicles per clump, weight of 100 grains, and the content of N and P stover. Results show that inoculantion, affect significantly on the number of panicles per hill, grain weight per panicle and the dry weight of grains per panicle.
KARAKTERISASI PISANG REJANG TETRAPLOID HASIL INDUKSI DENGAN ORYZALIN
Authors: Poerba Yuyu Suryasari , Handayani T , Witjaksono Witjaksono 
Published: October 2019

Triploid banana cultivar is the most desirable cultivar in banana industry because of its higher yield compared to its diploid cultivar. The triploid cultivar can be produced by crossing tetraploid with diploid cultivar. However, tetraploid banana cultivar is rarely existed naturally. Induced tetraploid of Pisang Rejang was produced using oryzalin. The present research was conducted to characterize tetraploid Pisang Rejang (Musa acuminata, AAAA genome) induced by in-vitro oryzalin treatment from diploid Pisang Rejang. Ploidy level, molecular and morphotaxonomic characters were observed. Ploidy identification of induced Pisang Rejang was conducted using Flowcytometer. Molecular characterization was done using RAPD and ISSR markers. Morphology characters were observed based on UPOV (2010). The results showed that tetraploid plants have similar genetic properties with their diploid controls as shown by genetic identity of 0.9901 – 0.9935. The tetraploids were differed from their diploid plants in plan habit and diameter of fruit. The tetraploid plants produce fewer suckers, drooping leaves and broader fruits compared to its diploid control.
KEANEKARAGAMAN KEONG DI PULAU ENGGANO, BENGKULU UTARA
Authors: Heryanto Heryanto 
Published: October 2019

Research on the diversity of snails in Enggano, Central Bengkulu was held on 16 April 2015 to 5 May 2015 by taking three sampling sites that are north-western part of Enggano (Kampung Bendung at Desa Banjarsari and Desa Meok), the centralpart of Enggano (Desa Malakoni) and the south-easternpart of Enggano (Desa Kaana). Sampling was conducted using purposive sampling while the analysis using graphical method and statistical cluster and t-test. This study found 722 specimens of snails which consists of 24 species from 17 families. Most of the snails found are in small size. Land snails in the forest dominates the north-easternpart as much as 88.2%. compared to freshwater snails (11.8%). Terrestrial and freshwater snails share amount 50% each in the forest of the central, while terrestrial snails still dominate in the forest of south-eastern part (land snails: snail freshwater = 71.4%: 28.6% ). The existence of land snails and freshwater can not be separated from the state forests themselves besides the condition of the soil and surface water as a result of the topography of the island
KUALITAS SEMEN BEKU DOMBA GARUT (Ovis aries) PENAMBAHAN SUKROSA DALAM PENGENCER SEMEN KUNING
Authors: Suharman Herdis 
Published: October 2019

The objective of this research was to examine the effect of sucrose in improving the quality of the plasma membrane intact and sperm motility of frozen semen of Garut ram. Semen was collected using artificial vagina weekly from six mature garut rams. Immediately after initial evaluation, fresh semen was divided into four parts and diluted with Tr s extender without sucrose (T0), Tris extender + sucrose 0.2g/100 ml (T1), Tris extender + sucrose 0.4g/100 ml (T2) and Tris extender + sucrose 0.6g/100 ml (T3), respectively. Results of this research showed that the percentage of sperm motility after thawing in T2 (49.00 ± 5.48%) was significantly (P<0.05) higher than T0 (42.00 ± 2.74%) but was not significantly difference (P>0.05) than T1 (46.00 ± 4.18%)and T3 (48.00 ± 4.47%). Evaluation of plasma membrane intact showed that T1 (62.33 ± 6.51%) was s gnificantly different (P <0.05) w h T0 (49.40 ± 2.19%) but was not significantly different (P> 0.05) than T2 (58.50 ± 4.97%) and T3 (56.40 ± 5.90%). In conclusion, he addition of sucrose in semen extender improved the quality of frozen semen of Garut ram. Concentration of 0.2g / 100 ml is the op ma dose to improve the quality of the plasma membrane intact and motility of spermatozoa during the freezing process.
C0-CULTURE OF AMYLOLYTIC FUNGI Aspergillus niger AND OLEAGINOUS YEAST Candida orthopsilosis ON CASSAVA WASTE FOR LIPID ACCUMULATION
Authors: Kanti Atit , Sudiana I Made 
Published: October 2019

The objective of study was to evaluate the effectiveness of co-culture between amylolytic fungi Aspergillus niger and lipid accumulating yeast Candida orthopsilosis for lipid accumulation on cassava waste. When grown in 5 % starch medium, all microbes was able to grow, but best growth was observed on Aspergillus niger, other microbes grew slower. Moderate growth was observed on Saccharomycopsis fibuliger. The two isolates were then selected for further studies. Aspergillus niger and Saccharomycopsis fibuliger were amylolytic microbes. The amylolytic activities were affected by temperature, pH and nitrogen sources. Optimum temperature and pH for enzyme production were 30°C and 7.0 respectively. Both yeast extract and sodium nitrate were good nitrogen sources for amylase production. On cassava waste, the highest biomass and total lipid content were obtained by co-culture of Aspergillus niger and lipid accumulating yeast Candida orthopsilosis. Major lipid composition was oleic acids and strearic acids. The ability of co-culture of Aspergillus niger and lipid accumulating yeast Candida orthopsilosis grew and accumulated lipid on cassava waste would suggest that these culture were candidate for biofuel production.