bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transcriptome Analysis of MDA-MB-231 Cells Treated with Fumosorinone Isolated from Insect Pathogenic Fungi
Authors: Liu Zhiqin , Tian Yingchao , Chen Queting , Zhang Gaotao , Li Chunqing , Luo Du-Qiang 
Published: December 2019

Background:
In our previous study, we have isolated a new compound, named Fumosorinone (FU)
from insect pathogenic fungi, and was found to inhibit proliferation, migration, and invasion of breast cancer
MDA-MB-231 cells.

Objective:
The aim of this study was to identify the underlying molecular mechanisms for FU effects on MDAMB-
231 cells.

Methods:
After MDA-MB-231 cells were treated with FU for 48h, RNA sequencing was used to identify the
effect of FU on the transcriptome of MDA-MB-231 cells. The validation of the relative expression of the selective
genes was done using quantitative real-time PCR (qRT-PCR).

Results:
The transcriptome results showed that 2733 genes were differentially expressed between the untreated
and the FU-treated cells, including 1614 up-regulated and 1119 down-regulated genes. The multiple genes are
associated with cancer cell growth, migration, and invasion. Functional analysis identified multitude of pathways
related to cancer, such as cell cycle, ECM–receptor interaction, p53 signaling pathway. We selected 4
upregulated and 9 downregulated genes, which are associated with breast cancer to verify their expression using
qRT-PCR. The validation showed that HSD3B1, ALOX5, AQP5, COL1A2, CCNB1, CCND1, VCAM-1,
PTPN1 and PTPN11 were significantly downregulated while DUSP1, DUSP5, GADD45A, EGR1 were upregulated
in FU-treated MDA-MB-231cells.

Conclusion:
These aberrantly expressed genes and pathways may play pivotal roles in the anti-cancer activity of
FU, and maybe potential targets of FU treatments for TNBC. Further investigations are required to evaluate the
FU mechanisms of anti-cancer action in vivo.
Congestion and Pollution, Vehicle Routing Problem of a Logistics Provider in Thailand
Authors: Moryadee Chanicha , Aunyawong Wissawa , Shaharudin Mohd Rizaimy 
Published: December 2019

Aim and Objective:
This study aims to minimise the travelling distance, operation cost in terms of fuel consumption, and CO2 emissions. It introduces the Time-Dependency Pollution-Routing Problem (TDPRP) with the implementation of the time-dependency and emission model, including constraints such as the limitation of vehicle capacity and vehicle’s speed during different time periods in Thailand. Furthermore, the time window constraint is applied for representing a more realistic model. The main objective is to minimise the total pollution generated because of transportation.

Methods:
The Genetic Algorithm (GA) and Tabu Search (TS) methods have been used to generate the optimal solution with a variety of experiments. The best solutions from all the experiments have been compared to the original solution in terms of the quality of the solution and the computation time.

Results:
The best solution was generated by using the TS method with 30,000 trials. The minimum of the total CO2 emissions was 183.9846 kilograms produced from all of the vehicles during transportation, nearly half from the current transportation plan, which produced 320.94 kilograms of CO2 emissions.

Conclusion:
The proposed model optimised both the route and schedules (multiple time periods) for a number of vehicles, for which the transportation during a fixed congestion period could be predicted to avoid traffic congestion and reduce the CO2 emission. Future research is suggested to add other specific algorithms as well as constraints in order to make the model more realistic.
Evidence-Based Data About Prevalence and Risk of Malignancy of Thyroid Incidentalomas Detected by Different PET Radiopharmaceuticals
Authors: Signore Giovanni , Albano Domenico , Giovanella Luca , Bertagna Francesco , Treglia Giorgio 
Published: December 2019

Background:
To date, several meta-analyses and systematic reviews have reported data
about the prevalence and risk of malignancy of thyroid incidentalomas detected by different PET radiopharmaceuticals.

Objective:
This article aims to summarize the published evidence-based data about the prevalence and
risk of malignancy of thyroid incidentalomas detected by different PET radiopharmaceuticals.

Methods:
A comprehensive computer literature search of systematic reviews and meta-analyses published
up to July 2019 in PubMed/MEDLINE and Cochrane library databases regarding the prevalence
and risk of malignancy of thyroid incidentalomas detected by different PET radiopharmaceuticals was
carried out.

Results:
We have summarized the data about prevalence and risk of malignancy of thyroid incidentalomas
detected by different PET radiopharmaceuticals (fluorine-18 fluorodeoxyglucose, radiolabelled
choline and prostate-specific membrane antigen) taking into account 8 evidence-based articles.

Conclusion:
Evidence-based data demonstrated that thyroid incidentalomas detected by different PET
radiopharmaceuticals are not infrequent and their risk of malignancy is not negligible, in particular if
focal pattern is evident at PET, thus requiring further clinical and instrumental evaluation.
Petanque: Apa saja faktor fisik penentu prestasinya?
Authors: Hanief Yulingga Nanda , Purnomo Ardhi Mardiyanto Indra 
Published: December 2019

Petanque merupakan permainan dengan melempar bola besi (BOSI) sedekat mungkin pada sasaran berupa bola kayu (cochonnet). Permainan ini menuntut akurasi yang tinggi. Tujuan penelitian ini adalah untuk mengetahui faktor fisik dominan penentu prestasi petanque. Penelitian ini merupakan deskriptif kuantitatif. Populasi sekaligus sampel pada penelitian ini adalah semua atlet petanque Kota Kediri yang berjumlah 15 atlet dengan usia rata-rata 15 tahun. Teknik pengambilan data berupa tes dan pengukuran dengan item tes yaitu tinggi badan, panjang lengan, panjang telapak tangan, kekuatan otot lengan, kelentukan pergelangan tangan, keseimbangan, power otot lengan, kekuatan peras tangan, dan koordinasi mata-tangan. Teknik analisis data menggunakan analisis konfirmatori dengan bantuan SPSS versi 23. Hasil penelitian menunjukkan bahwa dari sembilan variabel yang dianalisis terdapat enam indikator variabel yang menunjukkan sebagai faktor fisik dominan penentu prestasi petanque, antara lain tinggi badan, panjang lengan, kekuatan otot lengan, kelentukan pergelangan tangan, keseimbangan, dan koordinasi mata-tangan. Untuk mendapatkan hasil lemparan yang akurat, faktor fisik tersebut harus menjadi penekanan utama setiap pelatih ketika berurusan dengan atlet pemula. Petanque: What are the physical factors that determine performance? AbstractPetanque is a game by throwing an iron ball (BOSI) as close as possible to the target in the form of a wooden ball (cochonnet). This game demands high accuracy. The purpose of this study was to determine the dominant physical factors determining petanque achievement. This research is a quantitative descriptive. The population as well as the sample in this study were all petanque athletes in the City of Kediri totaling 15 athletes with an average age of 15 years. Data collection techniques in the form of tests and measurements with test items namely height, arm length, palm length, arm muscle strength, wrist flexion, balance, arm muscle power, squeeze strength, and eye-hand coordination. The data analysis technique used explanatory analysis with the help of SPSS version 23. The results showed that of the nine variables analyzed there were six indicator variables that showed as dominant physical factors determining petanque achievement, including height, arm length, arm muscle strength, wrist flexion. , balance and eye-hand coordination. To get accurate toss results, these physical factors must be the main emphasis of every coach when dealing with novice athletes.
The NASSS-CAT Tools for Understanding, Guiding, Monitoring, and Researching Technology Implementation Projects in Health and Social Care: Protocol for an Evaluation Study in Real-World Settings
Authors: Greenhalgh Trisha , Maylor Harvey , Shaw Sara , Wherton Joseph , Papoutsi Chrysanthi , Betton Victoria , Nelissen Natalie , Gremyr Andreas , Rushforth Alexander , Koshkouei Mona , Taylor John 
Published: December 2019

Background
Projects to implement health care and social care innovations involving technologies are typically ambitious and complex. Many projects fail. Greenhalgh et al’s nonadoption, abandonment, scale-up, spread, and sustainability (NASSS) framework was developed to analyze the varied outcomes of such projects.

Objective
We sought to extend the NASSS framework to produce practical tools for understanding, guiding, monitoring, and researching technology projects in health care or social care settings.

Methods
Building on NASSS and a complexity assessment tool (CAT), the NASSS-CAT tools were developed (in various formats) in seven co-design workshops involving 50 stakeholders (industry executives, technical designers, policymakers, managers, clinicians, and patients). Using action research, they were and are being tested prospectively on a sample of case studies selected for variety in conditions, technologies, settings, scope and scale, policy context, and project goals.

Results
The co-design process resulted in four tools, available as free downloads. NASSS-CAT SHORT is a taster to introduce the instrument and gauge interest. NASSS-CAT LONG is intended to support reflection, due diligence, and preliminary planning. It maps complexity through stakeholder discussion across six domains, using free-text open questions (designed to generate a rich narrative and surface uncertainties and interdependencies) and a closed-question checklist; this version includes an action planning section. NASSS-CAT PROJECT is a 35-item instrument for monitoring how subjective complexity in a technology implementation project changes over time. NASSS-CAT INTERVIEW is a set of prompts for conducting semistructured research or evaluation interviews. Preliminary data from empirical case studies suggest that the NASSS-CAT tools can potentially identify, but cannot always help reconcile, contradictions and conflicts that block projects’ progress.

Conclusions
The NASSS-CAT tools are a useful addition to existing implementation tools and frameworks. Further support of the implementation projects is ongoing. We are currently producing digital versions of the tools, and plan (subject to further funding) to establish an online community of practice for people interested in using and improving the tools, and hold workshops for building cross-project collaborations.

International Registered Report Identifier (IRRID)
DERR1-10.2196/16861
El "verdadero antemural de la Nueva España". Dos propuestas para la defensa de las Californias novohispanas
Authors: Altable Francisco 
Published: December 2019

<p>La historia de las Californias novohispanas se inserta en el largo proceso de expansión colonialista de España. A lo largo de años y siglos, el interés por consolidar el dominio territorial de esa región estuvo marcado por los grandes mitos en torno de sus riquezas naturales, de su cercanía al fantástico estrecho de Anián y del comercio transpacífico con el Asia oriental. De ahí que la defensa militar de sus costas adquiriera una importancia capital con claras implicaciones geopolíticas, religiosas y económicas. El presente artículo aborda dos inéditas propuestas de carácter estratégico-militar, ambas con el objeto de asegurar la soberanía española en esa parte del mundo y sentar las bases de su poblamiento civil.</p>
Lilium longiflorum Plant Growth with a combination of Naphthylacetic Acid (NAA) and 6Benzylaminopurine (BAP) In Vitro
Authors: Fauziah Rosita Husnun , Kusmiyati Florentina , Anwar Syaiful 
Published: December 2019

This research was aim to identify the effect of Naphthylacetic Acid (NAA) and 6-Benzylaminopurin (BAP) on growth of Lilium longiflorum planlet. The research method was Completely Randomized Design Factorial 4 x 4 with 4 replication. First factor was concentration of NAA, consist of A0: 0 mg/l, A1: 0,75 mg/l, A2: 1,5 mg/l, and A3: 2,25 mg/l. Second factor was concentration of BAP, consist of : B0: 0 mg/l, B1: 0,3 mg/l, B2: 0,6 mg/l, and B3: 0,9 mg/l. The observed parameters were number of shoots, plant height, number of leaves, number of roots, and percentage of contamination. Data were process by Analysis of Variance and continued by Least Significance Different (LSD). The result showed that combination of NAA and BAP did not significantly affect the growth parameter of Lilium longiflorum. BAP treatment significantly affected the number of shoots and number of leaves. The number of roots only observed in NAA treatment; 0,3 mg/l BAP + 0,75 mg/l NAA; 0,3 mg/l BAP + 1,5 mg/l NAA; and 0,6 mg/l BAP + 0,75 mg/l NAA. Percentage of fungal contamination was 7,5%. The conclusion of the result were the treatment 0f 0,3 mg/l BAP + 0,75 mg/l NAA was the best treatment for growth of Lilium longiflorum planlets with no contamination.
CARACTERÍSTICAS DEL FENÓMENO EMPRENDEDOR EN LOS MUNICIPIOS DE PUEBLA Y TLAXCALA, MÉXICO
Authors: Pérez Paredes Alfredo , Cruz de los Ángeles José Aurelio , Ramírez Elías Gloria 
Published: December 2019

El tema del emprendimiento empresarial actualmente es de suma relevancia, toda vez que permite la generación de nuevas empresas y fuentes de empleo que contribuyen de manera importante al producto interno bruto (PIB).
Para el presente trabajo de investigación, se tomó como objeto de estudio a los emprendedores de los municipios de Puebla y Tlaxcala, considerando que, por la cercanía de los ayuntamientos, las condiciones de tipo económico, político y social, pueden ser similares. Se pretende, por lo tanto, indagar cuáles son las principales características que tienen los emprendedores en estos municipios para la generación de nuevas empresas e identificar las causas por las cuales se generan altas tasas de mortalidad.
Se utilizó una encuesta que se aplicó a los emprendedores para identificar sus principales características; el instrumento utilizado se conformó de 11 ítems y en total se aplicaron 366 encuestas en cada municipio.
PENGEMBANGAN MODEL BLENDED LEARNING BERBASIS LEARNING MANAGEMENT SYSTEM DI MTS. MUHAMMADIYAH SANINGBAKAR SOLOK
Authors: Adri Muhammad , Sriwahyuni Titi , Dewi Ika Parma 
Published: December 2019

Blended learning is one of model for integration of pedagogic and information and communication technology (ICT) skill of teacher. The community service program aims are to develop an integrated learning environment using Learning Management Sytem and incraasing teacher skill on utilize of ICT as a supporting tool to quality improvement of teaching and learning process at MTs. Muhammadiyah Saningbakar. The ICT skill as well as define on National Curriculum Nasional 2013 become an integrated skill for every teacher. In this programme, two focus activity are defined, first activity is redesign ICT training for teaching and second is development an online learning environment based-on learning management system (LMS) as delivery medium on blended learning. Redesign ICT training including some of main competencies are 1) Course material development, 2) Word Processing, 3) Presentation design, 4) content development, 5) online learning and 6) applied strategic of blended learning. Those skill is needed to fullfill of successfullness requirement of blended learning. Development an online learning management system is using most popular open source course LMS named by Moodle. To achieve development goal some activity is applied as folows : 1) Define an domain name system for MTs. Muhammadiyah Saningbakar, 2) Get a hosting services as online storage for online environment, 3) LMS installation and Configiration, 4) User management, 5) Course and teacher settings and 6) Content uploading and delivery using LMS. Blended learning environment is developed based on MTs. M curriculum, and defined on course setting of LMS into 3 catagories : Islamic, Adaptive, and Subtantive. And each category containing some course which are available on LMS and accessed by student using networking.
Keyword: Blended Learning, ICT Skill, Integrated Learning Environment, LMS, Online Learning
Epidemiology and mortality in an Italian region after the adoption of the universal definition of myocardial infarction
Authors: Di Chiara Antonio , Clagnan Elena , Valent Francesca 
Published: December 2019

Aims
The aim of the study is to validate at the biochemical level (presence of myocardial damage) the discharge diagnosis code ICD-9-CM 410.x1, and to compare the acute myocardial infarction (AMI) epidemiology based on pure administrative data with the epidemiology based on troponin and clinical data.

Methods
The health-related administrative databases of the Italian Region Friuli Venezia Giulia were used as the source of information. All the databases are anonymous and can be linked with each other at the individual patient level through a univocal stochastic key. Two methods were used to assess incidence in 2017: the first used the main hospital discharge diagnosis, validated by biochemical myocardial necrosis; the second identified from the cohort of all patients with any myocardial injury those with ischemic origin.

Results
The positive-predictive value of the clinical diagnosis of AMI (410.x1), validated at the biochemical level, was 96.2%.
About 40% of patients with a not trivial biochemical myocardial injury and an ischemic heart disease diagnosis (e.g. 411) were discharged without either ST-elevation myocardial infarction (STEMI) or non-ST elevation myocardial infarction (NSTEMI) diagnosis, leading to a sensitivity of clinical discharge diagnosis of 47.6%.
Thirty-day and 90-day mortality at multivariate analysis resulted respectively, 1.8 and 4.0% in NSTEMI, 6.6 and 9.8% in STEMI, 8.8 and 12.2% in patients with biochemical AMI and discharge diagnosis other than 410.x1.

Conclusion
Pure administrative data (clinical discharge diagnosis) are today insufficient to catch the whole hospital epidemiology of myocardial infarction missing an important proportion of AMI with an adverse prognosis comparable with STEMI.
Biological Activity of Au(III) Complexes
Authors: Stankovic Ana S. , Kasalovic Marijana P. , Bukonjic Andriana M. , Mijajlovic Marina Z. , Tomovic Dusan Lj. , Nikolic Milos V. 
Published: December 2019

Abstract
The earliest therapy use of gold compounds dated in 2500 AD. In 1960s gold compounds were used in the treatment of rheumatoid arthritis, discoid lupus erythematosus, etc. Although the compounds of Au(I) are well-known and used in the modern medicine, new complexes of Au(III) and ligands with different donor atoms are synthetized and examined for their biological (antitumor, antimicrobial) activity. So far conducted studies show that complexes with Au(III) as central metal ions have greater activity compared with free ligands. Future syntheses should be based on the increase of biological activity with complexes that have necessary stability under physiological conditions.
Potential Benefits of Pre-emptive PGx Testing in Male Breast Cancer Patients
Authors: Randles Heather , Abraham Nina , Schuh Michael J. 
Published: December 2019

The objective is to report a case of recurrent breast cancer in a poor CYP2D6 metabolizer male patient on tamoxifen, and how pharmacogenomic (PGx) testing can play an important role in selecting appropriate adjuvant endocrine therapy.
The case examined here is a 60-year-old white male diagnosed with recurrence of breast cancer. The patient was prescribed tamoxifen four years prior as adjuvant endocrine therapy after initial treatment with surgery. PGx testing ordered at the time of recurrence revealed patient is a poor metabolizer of CYP2D6, which may decrease the efficacy of tamoxifen. The results prompted a change in therapy to an aromatase inhibitor (AI).
This case illustrates the potential benefits of preemptive PGx testing in a male breast cancer patient to assist in selecting appropriate adjuvant therapy based on how the patient metabolizes medications. In addition, PGx testing encourages patient involvement by emphasizing the association of genetics in determining treatment. The ultimate goal in performing these tests is to individualize treatment to improve safety and efficacy while minimizing adverse drug reactions.

Article Type: Case Study
PENERAPAN MODEL COOPERATIVE INTEGRATED READING AND COMPOSITION (CIRC) UNTUK MENINGKATKAN KEMAMPUAN MEMBACA PEMAHAMAN SISWA SMP NEGERI 6 MELIAU
Authors: Syaparani Syaparani 
Published: December 2019

AbstractThis research background is based on the fact that education of reading still low and provide direction and efforts which was done by teacher in implementation model cooperative integrated reading and composition of student class VII. Research use deskriptif method, with qualitative research approach and classroom action research design.The research subjects were students in class VII of Meliau Middle School 6 as many as 30 people. Data sources are obtained from students and teachers in the form of observation guidelines, written tests, and documentation.Implementation cooperative integrated reading and composition models successfully improve students' reading comprehension skills. The completeness of the group's average value in the first cycle is 73,4% and cycle second 87,3%, while the individual completeness in the first cycle is 77%, and cycle second 100%. Keywords: Cooperative integrated reading and composition, understanding reading ability
PENINGKATAN KETERAMPILAN MENGIDENTIFIKASI UNSUR INTRINSIK CERPEN MENGGUNAKAN MODEL KOOPERATIF TIPE PAIR CHECK
Authors: Yusvia Dona Yolanda , Martono Martono , Priyadi A. Totok 
Published: December 2019

AbstractA general problem in this research is how the improvement of identifying the intrinsic elements skills of short story using the cooperative model type of Pair Check at the students of XI IS4 SMA Santu Petrus Pontianak?". This was a researcher’s effort to improve the identifying the intrinsic elements skills of short story of students and provided a new variation in learning process as well as resolving students’ learning difficulties in in increasing the students’ score.This research used qualitative descriptive method form. The procedures in this research was a classroom action research (CAR) by four stages, there are action planning (planning), implementation of the action (action), observations (observation), and reflection (reflecting).Based on the results of data analysis in this research was known that the application of identifying the intrinsic elements of short story by using the cooperative learning model type of pair check can improve student learning outcomes. After done with the action I students’ average was 69,75 with the highest score was 89, action II with the students’ average score was 74,33 with average score was 89, and the action III, students’ average score was 83.33%with the highest score was 89. Keywords: Cooperative, Improvement, Model, Pair Check
Analisis Kesejahteraan Driver Go-Jek di Kota Padang (Pendekatan Objektif dan Subjektif)
Authors: Andrianus Fery , Yunekha Geneo 
Published: December 2019

<p>Go-Jek sebagai ojek online menjadi transportasi alternatif. Hampir tiap hari warga kota padang bertambah menjadi driver Go-Jek. Kondisi ini menunjukkan tingginya permintaan terhadap Go-Jek. Penelitian ini mengkaji bagaimana tingkat kesejahteraan driver Go-Jek di Kota Padang. Analisis yang digunakan adalah pendekatan objektif dan subjektif. Sampel penelitian terdiri dari 100 orang driver Go-jek. Hasil penelitian dengan pendekatan objektif dan subjektif menunjukkan driver Go-Jek sudah sejahtera. Pendapatan driver Go-Jek rata-rata per bulan di atas Upah Minimum Provinsi. Kesejahteraan driver Go-Jek juga dipengaruhi variabel non materi. Hasil ini menunjukkan bahwa driver Go-Jek merupakan menjadi alternatif dan peluang untuk menyerap tenaga kerja di Kota Padang</p>