bluuebunny/crossref_metadata_2025_split huggingface dataset:
Acyl-CoA-binding protein (ACBP): a phylogenetically conserved appetite stimulator
Authors: Charmpilas Nikolaos , Ruckenstuhl Christoph , Sica Valentina , Büttner Sabrina , Habernig Lukas , Dichtinger Silvia , Madeo Frank , Tavernarakis Nektarios , Bravo-San Pedro José M. , Kroemer Guido 
Published: January 2020

AbstractRecently, we reported that, in mice, hunger causes the autophagy-dependent release of a protein called “acyl-CoA-binding protein” or “diazepam binding inhibitor” (ACBP/DBI) from cells, resulting in an increase in plasma ACBP concentrations. Administration of extra ACBP is orexigenic and obesogenic, while its neutralization is anorexigenic in mice, suggesting that ACBP is a major stimulator of appetite and lipo-anabolism. Accordingly, obese persons have higher circulating ACBP levels than lean individuals, and anorexia nervosa is associated with subnormal ACBP plasma concentrations. Here, we investigated whether ACBP might play a phylogenetically conserved role in appetite stimulation. We found that extracellular ACBP favors sporulation in Saccharomyces cerevisiae, knowing that sporulation is a strategy for yeast to seek new food sources. Moreover, in the nematode Caenorhabditis elegans, ACBP increased the ingestion of bacteria as well as the frequency pharyngeal pumping. These observations indicate that ACBP has a phylogenetically ancient role as a ‘hunger factor’ that favors food intake.
Oligodendrocyte precursor cells transplantation protects blood–brain barrier in a mouse model of brain ischemia via Wnt/β-catenin signaling
Authors: Wang Liping , Geng Jieli , Qu Meijie , Yuan Fang , Wang Yuyang , Pan Jiaji , Li Yongfang , Ma Yuanyuan , Zhou Panting , Zhang Zhijun , Yang Guo-Yuan 
Published: January 2020

AbstractBlood–brain barrier damage is a critical pathological feature of ischemic stroke. Oligodendrocyte precursor cells are involved in maintaining blood–brain barrier integrity during the development. However, whether oligodendrocyte precursor cell could sustain blood–brain barrier permeability during ischemic brain injury is unknown. Here, we investigate whether oligodendrocyte precursor cell transplantation protects blood–brain barrier integrity and promotes ischemic stroke recovery. Adult male ICR mice (n = 68) underwent 90 min transient middle cerebral artery occlusion. After ischemic assault, these mice received stereotactic injection of oligodendrocyte precursor cells (6 × 105). Oligodendrocyte precursor cells transplantation alleviated edema and infarct volume, and promoted neurological recovery after ischemic stroke. Oligodendrocyte precursor cells reduced blood–brain barrier leakage via increasing claudin-5, occludin and β-catenin expression. Administration of β-catenin inhibitor blocked the beneficial effects of oligodendrocyte precursor cells. Wnt7a protein treatment increased β-catenin and claudin-5 expression in endothelial cells after oxygen–glucose deprivation, which was similar to the results of the conditioned medium treatment of oligodendrocyte precursor cells on endothelial cells. We demonstrated that oligodendrocyte precursor cells transplantation protected blood–brain barrier in the acute phase of ischemic stroke via activating Wnt/β-catenin pathway. Our results indicated that oligodendrocyte precursor cells transplantation was a novel approach to the ischemic stroke therapy.
Novel HDAC inhibitor Chidamide synergizes with Rituximab to inhibit diffuse large B-cell lymphoma tumour growth by upregulating CD20
Authors: Guan Xu-Wen , Wang Hua-Qing , Ban Wei-Wei , Chang Zhi , Chen Hai-Zhu , Jia Li , Liu Feng-Ting 
Published: January 2020

AbstractLoss of CD20 is a major obstacle for the retreatment of relapsed/refractory diffuse large B cell lymphoma (DLBCL) with Rituximab-associated regimens. Histone deacetylation causes gene silencing and inhibits CD20 expression. Chidamide is a novel inhibitor for histone deacetylases (HDACs). We hypothesize that Chidamide could overcome Rituximab-mediated down-regulation of CD20 and facilitate Rituximab-induced killing. In this study, we determine the mechanism of synergy of Chidamide with Rituximab in DLBCL using in vitro and in vivo models. We found that the levels of CD20 protein surface expression on five DLBCL cell lines were significantly and positively correlated with the sensitivities of cells to Rituximab. Treatment with Rituximab significantly reduced CD20 surface expression at the protein levels. RNA sequencing showed that Chidamide significantly increased expression of more than 2000 transcriptomes in DLBCL cells, around 1000 transcriptomes belong to the cell membrane and cell periphery pathways, including MS4A1. Chidamide significantly increased CD20 surface expression in DLBCL cell lines. Combination with Chidamide significantly synergized Rituximab-induced cell death in vitro and significantly inhibited tumour growth in DLBCL-bearing xenograft mice. A patient with relapsed/refractory DLBCL achieved a complete response after three cycles combined treatment with Chidamide and Rituximab. In conclusion, our data demonstrate for the first time that inhibition of HDACs by Chidamide significantly enhanced Rituximab-induced tumour growth inhibition in vitro and in vivo. We propose that CD20 surface expression should be used clinically to evaluate treatment response in patients with DLBCL. Chidamide is a promising sensitizer for the retreatment of DLBCL with Rituximab.
Off-target effect of the BMI1 inhibitor PTC596 drives epithelial-mesenchymal transition in glioblastoma multiforme
Authors: Flamier Anthony , Abdouh Mohamed , Hamam Rimi , Barabino Andrea , Patel Niraj , Gao Andy , Hanna Roy , Bernier Gilbert 
Published: January 2020

AbstractGlioblastoma multiforme (GBM) is an incurable primary brain tumor containing a sub-population of cancer stem cells (CSCs). Polycomb Repressive Complex (PRC) proteins BMI1 and EZH2 are enriched in CSCs, promoting clonogenic growth and resistance to genotoxic therapies. We report here that when used at appropriate concentrations, pharmaceutical inhibitors of BMI1 could efficiently prevent GBM colony growth and CSC self-renewal in vitro and significantly extend lifespan in terminally ill tumor-bearing mice. Notably, molecular analyses revealed that the commonly used PTC596 molecule targeted both BMI1 and EZH2, possibly providing beneficial therapeutic effects in some contexts. On the other hand, treatment with PTC596 resulted in instant reactivation of EZH2 target genes and induction of a molecular program of epithelial–mesenchymal transition (EMT), possibly explaining the modified phenotype of some PTC596-treated tumors. Treatment with a related but more specific BMI1 inhibitor resulted in tumor regression and maintenance of cell identity. We conclude that inhibition of BMI1 alone is efficient at inducing GBM regression, and that dual inhibition of BMI1 and EZH2 using PTC596 may be also beneficial but only in specific contexts.
Detection methods for influenza A H1N1 virus with special reference to biosensors: a review
Authors: Ravina  , Dalal Anita , Mohan Hari , Prasad Minakshi , Pundir C.S. 
Published: January 2020

Abstract
H1N1 (Swine flu) is caused by influenza A virus, which is a member of Orthomyxoviridae family. Transmission of H1N1 occurs from human to human through air or sometimes from pigs to humans. The influenza virus has different RNA segments, which can reassert to make new virus strain with the possibility to create an outbreak in unimmunized people. Gene reassortment is a process through which new strains are emerging in pigs, as it has specific receptors for both human influenza and avian influenza viruses. H1N1 binds specifically with an α-2,6 glycosidic bond, which is present in human respiratory tract cells as well as in pigs. Considering the fact of fast multiplication of viruses inside the living cells, rapid detection methods need an hour. Currently, WHO recommended methods for the detection of swine flu include real-time PCR in specific testing centres that take 3–4 h. More recently, a number of methods such as Antigen–Antibody or RT-LAMP and DNA biosensors have also been developed that are rapid and more sensitive. This review describes the various challenges in the diagnosis of H1N1, and merits and demerits of conventional vis-à-vis latest methods with special emphasis on biosensors.
A Novel Splice-Site Variant in SLC12A6 Causes Andermann Syndrome without Agenesis of the Corpus Callosum
Authors: Al Shibli Naema , Al-Maawali Almundher , Elmanzalawy Alaa , Al-Nabhani Maryam , Koul Roshan , Gabr Ahlam , Al Murshedi Fathiya 
Published: January 2020

AbstractAndermann syndrome, otherwise known as agenesis of the corpus callosum with peripheral neuropathy (ACCPN), is an autosomal recessive motor and sensory neuropathy known to be associated with ACC and mild-to-moderate intellectual disability. We present a 7-year-old girl with infantile-onset hypotonia, mild intellectual disability, and severe motor and sensory demyelinating peripheral neuropathy. Brain magnetic resonance imaging showed intact corpus callosum. Whole exome sequencing showed a novel splice-site pathogenic variant in the SLC12A6 gene. We confirm that ACC is not a mandatory feature and suggest that the term ACCPN may be misleading.
Bilateral Hidden Isokinetic Quadriceps Performance before and after MPFL Reconstruction in Pediatric Patients
Authors: Małecki Krzysztof , Niedzielski Kryspin , Flont Paweł , Fabis-Strobin Anna , Fabis Jarosław 
Published: January 2020

AbstractWe perform prospective study to evaluate the isokinetic performance of quadriceps before and 1 year after medial patella-femoral ligament (MPFL) reconstruction with the adductor magnus tendon in the case of recurrent patellar dislocation. The present study is the first to describe the isokinetic function of the quadriceps of the involved and uninvolved extremity, in such a wide range. The MPFL is a crucial passive stabilizer of patella and, along with the conditions of the anatomical shape of the femoral-patellar joint and the function of quadriceps, influences the overall patellar stability. However, only a few studies have examined indirectly or directly the function of quadriceps. A total of 27 patients (average age at surgery was 15.8 years) with recurrent monolateral patellar dislocation were treated with MPFL reconstruction using the adductor magnus tendon. In the study group, healthy and operated quadriceps were evaluated for the following parameters at the velocities of 60 and 180 deg/s before surgery and in the follow-up examination: peak torque, peak torque to body weight, time to peak torque, peak torque angle, torque in 30 degree of the knee flexion (TQ 30 degree), and the torque in the first 180 milliseconds (TQ 180). Preoperative patellar instability and its normalization after MPFL reconstruction have no impact on the isokinetic quadriceps index value which depends on the time and degree of inactivity as well as implementation of appropriate physiotherapy. The increase in the quadriceps muscle strength of a healthy limb is responsible for the persistence of muscle isokinetic imbalance after MPFL reconstruction in pediatric patients. This is a level 2b study.
Use of a Mandatory Clinical Decision Unit Reduces Readmission Rates following Total Joint Arthroplasty
Authors: Manoli Arthur , Markel Jacob F. , Pizzimenti Natalie M. , Markel David C. 
Published: January 2020

AbstractReadmission penalties have encouraged the implementation of protocols to reduce readmission rates. We hypothesized that by keeping postoperative patients, who return to the emergency department (ED) in a clinical decision unit (CDU) until being evaluated by the orthopaedic team, there would be a reduction in the readmission rate after total joint arthroplasty (TJA) at our institution. Our institution mandated the use of the CDU for all potential orthopaedic TJA readmissions. A retrospective review of prospectively collected data was performed on 365 patients who presented to the ED after either total hip arthroplasty (THA) or total knee arthroplasty (TKA). Patients presenting in the year prior to the implementation of the CDU program were compared with patients presenting in the year after implementation. Demographics, length of stay, comorbidities, and 30-day readmission rates were recorded. Additionally, a financial analysis was performed. Overall, for THA and TKA, there were a combined 141 ED visits prior to the implementation of the CDU program and 224 afterward; of these, 40 were readmitted before the CDU program and only 13 were readmitted afterward (p < 0.01). The financial analysis found that the overall 90-day cost for patients in the postoperative period was nearly $800 lower on average (p = 0.027) post-CDU implementation.During the first year of the CDU project at our institution, we significantly reduced the readmission rates following TJA and demonstrated significant cost saving. This is a Level III, prognostic study.
Michigan Ophthalmology Pipeline: Exploring a Mentorship Model to Increase Diversity in Ophthalmology
Authors: Shaner Mason A. , Kaplan Ariane , Sesi Christopher , Manjunathan Abhishek , Lacy Gabrielle D. , Prisk Lauren , Crear Jara , Sugar Alan , Elam Angela , Mian Shahzad I. 
Published: January 2020

Abstract
Background Ethnic concordance between physicians and patients improves compliance and therapeutic benefit. Current literature shows a lack of diversity within ophthalmology. Thus, we aimed to develop a longitudinal mentorship program between first year ophthalmology residents (PGY2s) and first year medical students (M1s) coming from minority communities underrepresented in medicine (URM) to provide early exposure to the field.
Methods M1 members of URM organizations were recruited and paired with PGY2 ophthalmology residents in a mentorship program between 2017 and 2019. All participants were surveyed twice annually.
Results All 2017 M1s stated increased interest in ophthalmology, felt “satisfied” or “very satisfied” with the program, and completed all requirements. At the year-end, the mean educational value of the program for 2017 M1s was rated 4.33/5, and interest in ophthalmology 4.67/5. Quality of the clinical experiences for 2017 PGY2s 3.5/5, and the overall effectiveness of the program 3.5/5. At the year-end, the average educational value of the program for 2018 M1s was 4.4/5, and interest in ophthalmology 4.0/5. Quality of the clinical experiences for 2018 PGY2s was 3.1/5, and the overall effectiveness of the program was 3.4/5.
Conclusion Our “pipeline” program represents an ongoing effort to increase URM interest in ophthalmology. Continued assessment to identify areas for growth and improvement can optimize the program to aid other programs in initiating efforts to tackle this important issue.
Posterior Cruciate Ligament Reconstruction in the Multiple Ligament Injured Knee
Authors: Kew Michelle E. , Miller Mark D. 
Published: January 2020

AbstractMultiligamentous knee injuries are challenging to treat and diagnose. Posterior cruciate ligament (PCL) injuries are commonly found in the constellation of injuries included in a multiligamentous knee injury and are caused by a posteriorly directed force on the proximal tibia with relation to the femoral condyles. A thorough history and physical examination should be performed to evaluate for associated neurovascular injuries and associated ligamentous, chondral, or bony injuries. Nonsurgical management is reserved for patients who are critically ill or have very low activity demands. Surgical reconstruction is recommended for most patients with multiligamentous knee injuries. The PCL reconstruction can be undertaken with several different graft options and reconstruction techniques, including the transtibial, arthroscopic tibial inlay, and open tibial inlay approach. The literature has a paucity of data regarding outcomes among the various reconstructive options, so the optimal surgical technique has not been established.
The 90-day Readmission Rate after Single-Bundle ACL Reconstruction Plus LET: Analysis of 2,559 Consecutive Cases from a Single Institution
Authors: Grassi Alberto , Costa Giuseppe Gianluca , Cialdella Sergio , Lo Presti Mirco , Neri Maria Pia , Zaffagnini Stefano 
Published: January 2020

AbstractThe aim of the present study is to examine the readmission rate within 90 days of a cohort of patients, who underwent an arthroscopic anterior cruciate ligament (ACL) reconstruction with a lateral extraarticular tenodesis, from a single highly specialized sports traumatology department. From our institutional database, we identified 2,559 patients (78.5% males and 21.5% females, with a mean age at surgery of 30.9 ± 11.5 years) who underwent primary ACL reconstruction with the same “over-the-top” technique plus lateral extraarticular tenodesis (LET) from January 2010 to December 2017. From this count, we extracted all patients who were readmitted within 90 days and focused on causes of readmission and reoperation rate. Moreover, a multivariate logistic regression was performed to identify possible variables, such as gender, age, and concomitant meniscus surgery, which could predict the risk of early readmission. From the aforementioned cohort, 58 patients (2.27%) were readmitted within 90 days from surgery after a mean time of 31 ± 23 days. The most common cause of readmission was fever and knee swelling (0.78%), followed by superficial infection (0.63%), deep infection (0.55%), and joint stiffness (0.23%). The patients' age and meniscal lesions requiring concomitant arthroscopic treatment were found to be correlated to an increased risk of early readmission. Superficial infections were more common in female patients (odds ratio [OR] = 3.01), whereas the meniscal treatment was also a significant risk factor specifically for deep infections (OR = 3.56). In conclusion, this technique of arthroscopic ACL reconstruction with LET showed a low readmission rate within 90 days from surgery. However, patients and physicians should be aware of the risk of serious complications, such as deep and superficial infections, mostly in female patients and in cases of concurrent meniscal treatments. This is a Level IV, retrospective case series study.
Performances for upward mobility: How Dutch workfare programmes function as temporal spaces of imagination
Authors: Arts Josien 
Published: January 2020

ABSTRACT
The gradual retreat of many governments from actively supporting secure labour relations and social security through welfare arrangements, and the related normalisation of precarious conditions, goes hand-in-hand with a promise of the attainability of ‘the good life’ through work. Workfare programmes are at the centre of this, as they are aimed at ‘improving’ welfare clients and their position in society through performing precarious work. Based on an ethnographic study of group workshops in three Dutch workfare programmes, this article shows how welfare clients are taught the promise of upward mobility through waged labour and are required to give ‘the right’ performances that might (in principle if, often, not in practice) potentially enable them to be successful on the post-Fordist labour market. I argue that these workshops function as temporal spaces of imagination in which adherence to the promise of upward mobility through paid work can best be understood as a form of post-Fordist affect, one that enacts, albeit temporarily, a resolution that is frequently lacking in real life.
Comparative analysis of the Coulomb barrier in heavy-ion collisions by the double-folding method
Authors: Ganiev O K , Nasirov A K 
Published: January 2020

Abstract
The double-folding formalism has been applied to calculate the nucleus-nucleus potential by the use of the effective nucleon-nucleon (Migdal) potential and the nuclear densities of the interacting nuclei presented as the Gaussian-type functions and polynomials. The Coulomb barrier heights obtained by this way and by different types of the proximity potentials have been compared. The deviations of the theoretical values of the Coulomb barrier from the values extracted from the experimental data are discussed. The capture cross section calculated for the reactions with the light projectiles is in good agreement with experimental values. The theoretical results obtained for the 16O+144Sm and 17O+144Sm reactions could reproduce a significant difference in the experimental results for these reaction, which is related with the extra neutron in 17O. The overestimation of the experimental data obtained from different sources by the curve of the theoretical results of the 40Ca+96Zr reactions is explained by the appearance of the slight hindrance to complete fusion, i.e the capture and fusion cross sections are not equal in this reaction.
Trivial band topology of ultra-thin rhombohedral Sb2Se3 grown on Bi2Se3
Authors: Matetskiy A V , Mararov V V , Kibirev I A , Zotov A V , Saranin A A 
Published: January 2020

Abstract
Thin films of rhombohedral Sb2Se3 with thicknesses from 1 to 5 quintuple layers (QL) were grown on Bi2Se3/Si(1 1 1) substrate. The electronic band structure of the grown films and the Sb2Se3/Bi2Se3 interface were studied using angle-resolved photoemission spectroscopy. It was found that while Sb2Se3 has an electronic band structure generally similar to that of Bi2Se3, there is no fingerprints of band inversion in it. Instead, the one-QL-thick Sb2Se3 films show direct band gap of about 80 meV. With growing film thickness, the Fermi level of the Sb2Se3 films gradually shifts by 200 meV for 5 QL-thick film revealing the band bending of the Sb2Se3/Bi2Se3 hetero-junction.
Magnetic-repulsion-coupled piezoelectric-film-based stretchable and flexible acoustic emission sensor
Authors: Feng Guo-Hua , Chiang Cheng-Yen 
Published: January 2020

Abstract
This paper presents a stretchable and flexible acoustic emission (AE) sensor composed of patterned upper, lower piezoelectric film foils, magnets and stereolithographic structures. The proposed device possesses the following novelties. The piezoelectric sensing structures with magnetic repulsion is effective for AE signal coupling. The lower piezoelectric serpentine-shaped AE detection structure with stretchable and flexible characteristics allows sensing various curved surfaces. Additionally, the contact force between the sensing target and AE sensor can be evaluated by monitoring the structural characteristic change of the upper piezoelectric structure of the AE sensor. The magnetic-repulsion-enhanced AE sensor exhibits a better bandwidth compared to that with only a lower AE sensing structure. Also, the fabricated sensor subjected to a sensing target force ranging from 4.98 to 14.85 mN resulting in a frequency change of the piezoelectric sensing beam of the upper foil from 20.073 to 20.135 kHz is verified. The developed AE sensor can open a new field for various applications. For instance, AE waves can be monitored without contacting the target (e.g. interfacing with air). The detection mechanism of AE waves by an action at a distance is successfully demonstrated.