bluuebunny/crossref_metadata_2025_split huggingface dataset:
Proteomic and transcriptomic profiling identifies mediators of anchorage-independent growth and roles of inhibitor of differentiation proteins in invasive lobular carcinoma
Authors: Tasdemir Nilgun , Ding Kai , Savariau Laura , Levine Kevin M. , Du Tian , Elangovan Ashuvinee , Bossart Emily A. , Lee Adrian V. , Davidson Nancy E. , Oesterreich Steffi 
Published: July 2020

AbstractInvasive lobular carcinoma (ILC) is a histological subtype of breast cancer with distinct molecular and clinical features from the more common subtype invasive ductal carcinoma (IDC). ILC cells exhibit anchorage-independent growth in ultra-low attachment (ULA) suspension cultures, which is largely attributed to the loss of E-cadherin. In addition to anoikis resistance, herein we show that human ILC cell lines exhibit enhanced cell proliferation in ULA cultures as compared to IDC cells. Proteomic comparison of ILC and IDC cell lines identified induction of PI3K/Akt and p90-RSK pathways specifically in ULA culture in ILC cells. Further transcriptional profiling uncovered unique upregulation of the inhibitors of differentiation family transcription factors ID1 and ID3 in ILC ULA culture, the knockdown of which diminished the anchorage-independent growth of ILC cell lines through cell cycle arrest. We find that ID1 and ID3 expression is higher in human ILC tumors as compared to IDC, correlated with worse prognosis uniquely in patients with ILC and associated with upregulation of angiogenesis and matrisome-related genes. Altogether, our comprehensive study of anchorage independence in human ILC cell lines provides mechanistic insights and clinical implications for metastatic dissemination of ILC and implicates ID1 and ID3 as novel drivers and therapeutic targets for lobular breast cancer.
Assessment of cortical reorganization and preserved function in phantom limb pain: a methodological perspective
Authors: Andoh Jamila , Milde Christopher , Diers Martin , Bekrater-Bodmann Robin , Trojan Jörg , Fuchs Xaver , Becker Susanne , Desch Simon , Flor Herta 
Published: July 2020

AbstractPhantom limb pain (PLP) has been associated with reorganization in primary somatosensory cortex (S1) and preserved S1 function. Here we examined if methodological differences in the assessment of cortical representations might explain these findings. We used functional magnetic resonance imaging during a virtual reality movement task, analogous to the classical mirror box task, in twenty amputees with and without PLP and twenty matched healthy controls. We assessed the relationship between task-related activation maxima and PLP intensity in S1 and motor cortex (M1) in individually-defined or group-conjoint regions of interest (ROI) (overlap of task-related activation between the groups). We also measured cortical distances between both locations and correlated them with PLP intensity. Amputees compared to controls showed significantly increased activation in M1, S1 and S1M1 unrelated to PLP. Neural activity in M1 was positively related to PLP intensity in amputees with PLP when a group-conjoint ROI was chosen. The location of activation maxima differed between groups in S1 and M1. Cortical distance measures were unrelated to PLP. These findings suggest that sensory and motor maps differentially relate to PLP and that methodological differences might explain discrepant findings in the literature.
Women’s socioeconomic position in ontogeny is associated with improved immune function and lower stress, but not with height
Authors: Rubika Anna , Luoto Severi , Krama Tatjana , Trakimas Giedrius , Rantala Markus J. , Moore Fhionna R. , Skrinda Ilona , Elferts Didzis , Krams Ronalds , Contreras-Garduño Jorge , Krams Indrikis A. 
Published: July 2020

AbstractImmune function, height and resource accumulation comprise important life history traits in humans. Resource availability models arising from life history theory suggest that socioeconomic conditions influence immune function, growth and health status. In this study, we tested whether there are associations between family income during ontogeny, adult height, cortisol level and immune response in women. A hepatitis B vaccine was administered to 66 young Latvian women from different socioeconomic backgrounds, and blood samples were then collected to measure the level of antibodies that the women produced in response to the vaccination. Cortisol levels were measured from plasma samples pre- and post-vaccination. Women from wealthier families had lower cortisol levels, and women from the highest family income group had the highest levels of antibody titers against hepatitis B vaccine. No significant relationships were observed between cortisol level and immune function, nor between family income and height. The results show that income level during ontogeny is associated with the strength of immune response and with psychoneuroendocrine pathways underlying stress perception in early adulthood. The findings indicate that the quality of the developmental niche is associated with the condition-dependent expression of immune function and stress response.
Health risk assessment of heavy metal pollution in a soil-rice system: a case study in the Jin-Qu Basin of China
Authors: Guo Bin , Hong Chunlai , Tong Wenbin , Xu Mingxing , Huang Chunlei , Yin Hanqin , Lin Yicheng , Fu Qinglin 
Published: July 2020

AbstractA regional field survey of a total of 109 pairs of soil and rice samples was conducted to evaluate the health risks posed by heavy metals in the Jin-Qu Basin, China. The studied soils are characterized by acid (pH in mean level of 5.5), carbon rich (soil organic matter in mean of 33.6 g kg−1) and mainly contaminated by Cd (42.2% samples exceeded the standard value of 0.3 mg kg−1 (GB15618-2018)). The spatial distributions of Cd, Pb and Zn exhibited similar geographic trends. 34% and 30% of the rice samples containing Cd and Pb exceeded the threshold value of 0.2 mg kg−1 (GB2762-2017), respectively. The risk estimation of dietary intake had a target hazard quotient value of Cd of 0.918 and a hazard index value for rice consumption of 2.141. Totally, Cd and Pb were found to be the main components contributing to the potential health risks posed by non-carcinogenic effects for local inhabitants.
The patterns of response of 11 regimens for infantile spasms
Authors: Mao Leilei , Kessi Miriam , Peng Pan , He Fang , Zhang Ciliu , Yang Lifen , Wu Liwen , Yin Fei , Peng Jing 
Published: July 2020

AbstractInfantile spasms (ISs) is a devastating form of an early infantile epileptic encephalopathy. The patterns of response of multiple regimens, and the difference in response rates for the cases who receive first-line therapies on time versus those who receive them after non-first-line therapies are unknown. We performed a study involving 314 ISs cases aiming to investigate the patterns of response of 11 regimens, and the difference in response rates for the cases received first-line therapies as first two regimens versus those who received other drugs prior to first-line options. As a result, the efficacy of each regimen was: the foremost two regimens; 36.99%, third; 10.27%, fourth; 6.16%, fifth; 5.48%, and from the sixth regimen onwards, each additional regimen added ≤ 2% probability of seizure freedom. There was a statistically significant difference in seizure freedom rates between cases received first-line therapies as first or second regimen versus those who received them later. Our study revealed for the first time that in ISs cases, seizure freedom is likely to be observed within the first five regimens, and an early administration of first-line therapies is superior to non-first-line options. These results will aid in management of ISs cases.
Transcription factor 21 expression in injured podocytes of glomerular diseases
Authors: Usui Joichi , Yaguchi Misa , Yamazaki Satoshi , Takahashi-Kobayashi Mayumi , Kawamura Tetsuya , Kaneko Shuzo , Seshan Surya V. , Ronco Pierre , Yamagata Kunihiro 
Published: July 2020

AbstractTranscription factor 21 (TCF21) is one of the essential transcription factors in kidney development. To elucidate its influence on glomerular disease, we have investigated TCF21 expression in human and rat kidney tissue, and its urinary concentration. Immunohistological analysis suggested the highest TCF21 expression in nephrotic syndrome along with the urinary protein level. Urinary TCF21 concentration in human showed a positive correlation with its podocyte expression level. In nephrotic rat models, TCF21 expression in podocytes increased along with the severity of nephrotic syndrome. Next, in vitro experiments using Tcf21-expressing murine podocyte cell line, we could observe some Tcf21-dependent effects, related with actin cytoskeleton dysregulation and apoptosis. Our study illustrated TCF21 expression changes in vivo and its in vitro-functional significance injured podocytes.
Identification of new regulatory genes through expression pattern analysis of a global RNA-seq dataset from a Helicobacter pylori co-culture system
Authors: Tubau-Juni Nuria , Bassaganya-Riera Josep , Leber Andrew , Zoccoli-Rodriguez Victoria , Kronsteiner Barbara , Viladomiu Monica , Abedi Vida , Philipson Casandra W. , Hontecillas Raquel 
Published: July 2020

AbstractHelicobacter pyloriis a gram-negative bacterium that persistently colonizes the human stomach by inducing immunoregulatory responses. We have used a novel platform that integrates a bone marrow-derived macrophage and liveH. pylorico-culture with global time-course transcriptomics analysis to identify new regulatory genes based on expression patterns resembling those of genes with known regulatory function. We have used filtering criteria based on cellular location and novelty parameters to select 5 top lead candidate targets. Of these, Plexin domain containing 2 (Plxdc2) was selected as the top lead immunoregulatory target. Loss of function studies with in vivo models ofH. pyloriinfection as well as a chemically-induced model of colitis, confirmed its predicted regulatory function and significant impact on modulation of the host immune response. Our integrated bioinformatics analyses and experimental validation platform has enabled the discovery of new immunoregulatory genes. This pipeline can be used for the identification of genes with therapeutic applications for treating infectious, inflammatory, and autoimmune diseases.
Maximum oxygen consumption and quantification of exercise intensity in untrained male Wistar rats
Authors: Qin Fei , Dong Yanan , Wang Songtao , Xu Minxiao , Wang Zhongwei , Qu Chaoyi , Yang Yan , Zhao Jiexiu 
Published: July 2020

AbstractThis study aimed to explore a valid test protocol for measuring VO2max in healthy untrained male Wistar rats of different ages and quantifying the exercise intensity (%VO2max) of running under different treadmill grades and speeds. The test protocols and %VO2max will provide a reference for the design of exercise intensity. We tested male Wistar rats aged 4 weeks, 10 weeks, 10 months and 16 months old with three test protocols (Procedure 1 [P1], 2 [P2] and 3 [P3]) for each age group to quantify VO2max. We analysed VO2max, respiratory exchange ratio and test duration to determine an optimal test protocol of VO2max for different age groups. We used the optimal test protocol to explore the changes in age-related VO2max. Finally, %VO2max of running under different treadmill speeds and grades was quantified. VO2max of Wistar rats decreased significantly after the age of 4 weeks (p < 0.05). The optimum VO2max can be induced by personalised protocols for different ages. In 4-week-old Wistar rats, the highest VO2max values were attained by P1 (104.4 ± 6.9 mL · kg−1 · min−1, p = 0.032). The highest VO2max value (84.7 ± 3.7 mL · kg−1 · min−1, p = 0.037) of 8-week-old Wistar rats was attained in P2. In 10-month-old Wistar rats, the highest VO2max value was obtained in P3 (63.3 ± 1.7 mL · kg−1 · min−1). This work could be used as a reference for assessing aerobic capacity in studies on exercise intervention with untrained male Wistar rats. However, the %VO2max measurements at various treadmill speeds and grades only apply to untrained male Wistar rats.
Correlation between paravertebral spread of injectate and clinical efficacy in lumbar transforaminal block
Authors: Bang Yu Jeong , Park Hue Jung , Sim Woo Seog , Lee Dae Won , Lee Jin Young 
Published: July 2020

AbstractThe potential paravertebral space includes spinal nerves, dorsal rami, rami communicants, and sympathetic chains. This study evaluated correlations between paravertebral spread of injectate and clinical efficacy in lumbar transforaminal block. We retrospectively analysed the data of 88 patients who received transforaminal blocks for lumbar radicular pain. We categorized patients into two groups: patients with ≥ 50% pain reduction on a numeric rating scale at 30 min following a block (responder group), and patients with < 50% pain reduction (non-responder group). Paravertebral spread of injectate was graded as limited to the anterior, middle, and posterior 1/3 of the anterolateral aspect of vertebral bodies; spread between the posterolateral margins of bodies and the posterior epidural space was considered no spread. Clinical and fluoroscopic data, perfusion index, temperature, and cold sensation were compared between the groups. Among 54 patients analysed, 26 (48.1%) experienced ≥ 50% and 28 (51.9%) < 50% pain reduction. Paravertebral spread occurred in 33 (61.1%) patients; 19 (57.6%) responders and 14 (42.4%) non-responders. On analysis, paravertebral spread, epidural spread patterns, perfusion index change ratios, temperature changes, and cold sensation changes showed no differences between responder and non-responder groups. Paravertebral spread occurred in 61.1%, with no correlation with the clinical efficacy of lumbar transforaminal block.
Evolutionary dynamics of the chloroplast genome sequences of six Colobanthus species
Authors: Androsiuk Piotr , Jastrzębski Jan Paweł , Paukszto Łukasz , Makowczenko Karol , Okorski Adam , Pszczółkowska Agnieszka , Chwedorzewska Katarzyna Joanna , Górecki Ryszard , Giełwanowska Irena 
Published: July 2020

AbstractThe complete plastome sequences of six species were sequenced to better understand the evolutionary relationships and mutation patterns in the chloroplast genome of the genus Colobanthus. The length of the chloroplast genome sequences of C. acicularis, C. affinis, C. lycopodioides, C. nivicola, C. pulvinatus and C. subulatus ranged from 151,050 to 151,462 bp. The quadripartite circular structure of these genome sequences has the same overall organization and gene content with 73 protein-coding genes, 30 tRNA genes, four rRNA genes and five conserved chloroplast open reading frames. A total of 153 repeat sequences were revealed. Forward repeats were dominant, whereas complementary repeats were found only in C. pulvinatus. The mononucleotide SSRs composed of A/T units were most common, and hexanucleotide SSRs were detected least often. Eleven highly variable regions which could be utilized as potential markers for phylogeny reconstruction, species identification or phylogeography were identified within Colobanthus chloroplast genomes. Seventy-three protein-coding genes were used in phylogenetic analyses. Reconstructed phylogeny was consistent with the systematic position of the studied species, and the representatives of the same genus were grouped in one clade. All studied Colobanthus species formed a single group and C. lycopodioides was least similar to the remaining species.
Cepstral Peak Prominence Values for Clinical Voice Evaluation
Authors: Murton Olivia , Hillman Robert , Mehta Daryush 
Published: July 2020

Purpose
The goal of this study was to employ frequently used analysis methods and tasks to identify values for cepstral peak prominence (CPP) that can aid clinical voice evaluation. Experiment 1 identified CPP values to distinguish speakers with and without voice disorders. Experiment 2 was an initial attempt to estimate auditory-perceptual ratings of overall dysphonia severity using CPP values.

Method

CPP was computed using the Analysis of Dysphonia in Speech and Voice (ADSV) program and Praat. Experiment 1 included recordings from 295 patients with medically diagnosed voice disorders and 50 vocally healthy control speakers. Speakers produced sustained /a/ vowels and the English language Rainbow Passage. CPP cutoff values that best distinguished patient and control speakers were identified. Experiment 2 analyzed recordings from 32 English speakers with varying dysphonia severity and provided preliminary validation of the Experiment 1 cutoffs. Speakers sustained the /a/ vowel and read four sentences from the Consensus Auditory-Perceptual Evaluation of Voice protocol. Trained listeners provided auditory-perceptual ratings of overall dysphonia for the recordings, which were estimated using CPP values in a linear regression model whose performance was evaluated using the coefficient of determination (
r
2
).

Results

Experiment 1 identified CPP cutoff values of 11.46 dB (ADSV) and 14.45 dB (Praat) for the sustained /a/ vowels and 6.11 dB (ADSV) and 9.33 dB (Praat) for the Rainbow Passage. CPP values below those thresholds indicated the presence of a voice disorder with up to 94.5% accuracy. In Experiment 2, CPP values estimated ratings of overall dysphonia with
r
2
values up to .74.

Conclusions
The CPP cutoff values identified in Experiment 1 provide normative reference points for clinical voice evaluation based on sustained /a/ vowels and the Rainbow Passage. Experiment 2 provides an initial predictive framework that can be used to relate CPP values to the auditory perception of overall dysphonia severity based on sustained /a/ vowels and Consensus Auditory-Perceptual Evaluation of Voice sentences.
Outcomes for Feeding Tube-Dependent Children With Oral Aversion in an Intensive Interdisciplinary Treatment Program
Authors: Bandstra Nancy F. , Huston Parker L. , Zvonek Kate , Heinz Carly , Piccione Emily 
Published: July 2020

Purpose
Feeding challenges in children are common, at times reaching a severity that requires the placement and long-term use of enteral feedings. A significant barrier to advancing the oral eating of some tube-dependent children is the presence of oral aversion. Although some research exists regarding the treatment of tube-dependent children who are averse to food or the process of eating, specifically, there has yet to be an examination of children who are truly “orally” averse—resisting not just the presentation of food or liquid but also nonnutritive stimuli presented extra- or intra-orally.

Method

Using a retrospective chart review, the current study aimed to examine the treatment outcomes of 18 feeding tube-dependent children with significant oral aversion (nine boys, nine girls;
M
age
= 46.7 months,
SD
= 20.0 months, range: 11.4–89.3 months) as compared to 29 tube-dependent, but nonorally averse, clinical controls. Children completed approximately 6–8 weeks of intensive interdisciplinary feeding treatment.

Results
Analyses revealed significant improvements in all measured treatment outcomes for both patient groups, including children's rates of acceptance and maladaptive mealtime behavior. Significant reductions in tube use were also observed across both groups, with tube utilization decreasing, on average, by 76.2% for orally averse and by 64.3% for nonorally averse children by program discharge.

Conclusion
Results demonstrate the therapeutic benefits of intensive interdisciplinary intervention for both groups of tube-dependent children, highlighting that orally averse children, believed to be an especially challenging subset of pediatric feeding patients, demonstrate similarly positive treatment responses. Interestingly, in this preliminary examination, orally averse children demonstrated significantly greater reductions in tube utilization following discharge when compared with their nonorally averse clinical peers.
Differential Neurobehavioral Effects of Cross-Modal Selective Priming on Phonetic and Emotional Prosodic Information in Late Second Language Learners
Authors: Kao Chieh , Zhang Yang 
Published: July 2020

Purpose
Spoken language is inherently multimodal and multidimensional in natural settings, but very little is known about how second language (L2) learners undertake multilayered speech signals with both phonetic and affective cues. This study investigated how late L2 learners undertake parallel processing of linguistic and affective information in the speech signal at behavioral and neurophysiological levels.

Method

Behavioral and event-related potential measures were taken in a selective cross-modal priming paradigm to examine how late L2 learners (
N
= 24,
M
age
= 25.54 years) assessed the congruency of phonetic (target vowel: /a/ or /i/) and emotional (target affect: happy or angry) information between the visual primes of facial pictures and the auditory targets of spoken syllables.

Results
Behavioral accuracy data showed a significant congruency effect in affective (but not phonetic) priming. Unlike a previous report on monolingual first language (L1) users, the L2 users showed no facilitation in reaction time for congruency detection in either selective priming task. The neurophysiological results revealed a robust N400 response that was stronger in the phonetic condition but without clear lateralization and that the N400 effect was weaker in late L2 listeners than in monolingual L1 listeners. Following the N400, late L2 learners showed a weaker late positive response than the monolingual L1 users, particularly in the left central to posterior electrode regions.

Conclusions
The results demonstrate distinct patterns of behavioral and neural processing of phonetic and affective information in L2 speech with reduced neural representations in both the N400 and the later processing stage, and they provide an impetus for further research on similarities and differences in L1 and L2 multisensory speech perception in bilingualism.
Long photometric cycle and disk evolution in the β Lyrae-type binary OGLE-BLG-ECL-157529
Authors: Mennickent R. E. , Garcés J. , Djurašević G. , Iwanek P. , Schleicher D. , Poleski R. , Soszyński I. 
Published: July 2020

Context. The subtype of hot algol semidetached binaries dubbed double periodic variables (DPVs) are characterized by a photometric cycle longer than the orbital one, whose nature has been related to a magnetic dynamo in the donor component controlling the mass transfer rate.
Aims. We aim to understand the morphologic changes observed in the light curve of OGLE-BLG-ECL-157529 that are linked to the long cycle. In particular, we want to explain the changes in the relative depth of primary and secondary eclipses.
Methods. We analyzed I and V-band OGLE photometric times series spanning 18.5 years and modeled the orbital light curve.
Results. We find that OGLE-BLG-ECL-157529 is a new eclipsing Galactic DPV of orbital period 24d​​.8, and that its long cycle length decreases in amplitude and length during the time baseline. We show that the changes in the orbital light curve can be reproduced considering an accretion disk of variable thickness and radius that surrounds the hottest stellar component. Our models indicate changes in the temperatures of the hot spot and the bright spot during the long cycle, and also in the position of the bright spot. This, along with the changes in disk radius, might indicate a variable mass transfer in this system.