bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Prognostic Model for Colon Cancer Patients Based on Eight Signature Autophagy Genes
Authors: Xu Jiasheng , Dai Siqi , Yuan Ying , Xiao Qian , Ding Kefeng 
Published: November 2020

ObjectiveTo screen key autophagy genes in colon cancer and construct an autophagy gene model to predict the prognosis of patients with colon cancer.MethodsThe colon cancer data from the TCGA were downloaded as the training set, data chip of GSE17536 as the validation set. The differential genes of the training set were obtained and were analyzed for enrichment and protein network. Acquire autophagy genes from Human Autophagy Database www.autophagy.lu/project.html. Autophagy genes in differentially expressed genes were extracted using R-packages limma. Using LASSO/Cox regression analysis combined with clinical information to construct the autophagy gene risk scoring model and divide the samples into high and low risk groups according to the risk value. The Nomogram assessment model was used to predict patient outcomes. CIBERSORT was used to calculate the infiltration of immune cells in the samples and study the relationship between high and low risk groups and immune checkpoints.ResultsNine hundred seventy-six differentially expressed genes were screened from training set, including five hundred sixty-eight up-regulated genes and four hundred eight down regulated genes. These differentially expressed genes were mainly involved: the regulation of membrane potential, neuroactive ligand-receptor interaction. We identified eight autophagy genes CTSD, ULK3, CDKN2A, NRG1, ATG4B, ULK1, DAPK1, and SERPINA1 as key prognostic genes and constructed the model after extracting the differential autophagy genes in the training set. Survival analysis showed significant differences in sample survival time after grouping according to the model. Nomogram assessment showed that the model had high reliability for predicting the survival of patients with colon cancer in the 1, 3, 5 years. In the high-risk group, the infiltration degrees of nine types of immune cells are different and the samples can be well distinguished according to these nine types of immune cells. Immunological checkpoint correlation results showed that the expression levels of CTLA4, IDO1, LAG3, PDL1, and TIGIT increased in high-risk groups.ConclusionThe prognosis prediction model based on autophagy gene has a good evaluation effect on the prognosis of colon cancer patients. Eight key autophagy genes can be used as prognostic markers for colon cancer.
Carotid Intima-Media Thickness in Healthy Children and Adolescents: Normative Data and Systematic Literature Review
Authors: Drole Torkar Ana , Plesnik Emil , Groselj Urh , Battelino Tadej , Kotnik Primoz 
Published: November 2020

Objectives: Early identification of children at risk of atherosclerosis is of paramount importance for implementing primary preventive measures addressing vascular health. Carotid intima-media thickness (cIMT) is a non-invasive biomarker of atherosclerosis. Semiautomatic radiofrequency-based software-guided technique quality intima-media thickness (RF-QIMT) was used to determine cIMT normative values in a healthy cohort of Caucasian children aged 6 to 18 years.Study design: In a cross-sectional study, data on age, chronic illness, medication use, and pubertal status was acquired by a questioner. Anthropometric and blood pressure measurements were performed by standardized methods and trained medical personnel. cIMT of the right common carotid artery far wall (1 centimeter proximal to bifurcation) was determined using a multifrequency (3–13 MHz) electronic linear array transducer SL1543, a portable ultrasound device (MyLab Gamma Esaote, Genoa, Italy), and RF-QIMT software. A systematic review of the published normal cIMT in children was done using PRISMA methodology, and identified normative values were compared to those obtained in the presented study.Results: 1137 non-obese normotensive children (males: n = 512; mean age 12.04 ± 3.52 years, females: n = 625, mean age 12.98 ± 3.83 years) were included. Gender-, age-, and height-specific mean cIMT percentile tables, percentile charts, and LMS tables for the RF-QIMT method were provided. They were comparable to the previously published data on mean cIMT gained by other validated ultrasound imaging techniques. cIMT increased with age, height, hip circumference, and BMI and was higher in males.Conclusions: Gender-, age-, and height-specific normative cIMT values, using the semiautomatic software-guided RF-QIMT technique, in children aged 6 to 18 years were developed and validated in respect to the previously published pediatric normative cIMT data. It is suggested that the investigated method could be used for the estimation of atherosclerotic risk in children, especially in epidemiological studies.
Long-Term Outcomes of a Phase I Study With UV1, a Second Generation Telomerase Based Vaccine, in Patients With Advanced Non-Small Cell Lung Cancer
Authors: Brunsvig Paal F. , Guren Tormod Kyrre , Nyakas Marta , Steinfeldt-Reisse Claudius H. , Rasch Wenche , Kyte Jon Amund , Juul Hedvig Vidarsdotter , Aamdal Steinar , Gaudernack Gustav , Inderberg Else Marit 
Published: November 2020

Human telomerase reverse transcriptase (hTERT) is a target antigen for cancer immunotherapy in patients with non-small cell lung cancer (NSCLC). We have tested a novel hTERT vaccine, UV1, designed to give high population coverage. UV1 is composed of three synthetic long peptides containing multiple epitopes identified by epitope spreading data from long-term survivors from previous hTERT vaccination trials. Eighteen non-HLA-typed patients with stage III/IV NSCLC with no evidence of progression after prior treatments, were enrolled in a phase I dose-escalation study of UV1 vaccination with GM-CSF as adjuvant, evaluating safety, immune response, and long-term clinical outcome. Treatment with UV1 was well tolerated with no serious adverse events observed. Seventeen patients were evaluable for tumor response; 15 patients had stable disease as best response. The median progression free survival (PFS) was 10.7 months, and the median overall survival (OS) was 28.2 months. The OS at 4 years was 39% (7/18). Five patients are alive (median survival 5.6 years), and none of these are known to have received checkpoint therapy after vaccination. UV1 induced specific T-cell responses in the majority (67%) of patients. Immune responses were dynamic and long lasting. Both immune response (IR) and OS were dose related. More patients in the highest UV1 dosage group (700 μg) developed IRs compared to the other groups, and the IRs were stronger and occurred earlier. Patients in this group had a 4-year OS of 83%. The safety and clinical outcome data favor 700 μg as the preferred UV1 dose in this patient population. These results provide a rationale for further clinical studies in NSCLC with UV1 vaccination in combination with immune checkpoint blockade.Clinical Trial Registrationhttps://www.clinicaltrials.gov, identifier NCT0178909.
Challenging Ultra Grain Refinement of Ferrite in Low-C Steel Only by Heat Treatment
Authors: Park Myeong-heom , Shibata Akinobu , Tsuji Nobuhiro 
Published: November 2020

It is well-known that grain refinement is one of the most effective ways to improve strength of metals without addition of alloying elements. In order to obtain bulky metals having ultrafine grained (UFG) microstructures with average grain sizes smaller than 1 μm, severe plastic deformation (SPD) processes have made a great success. However, there are still big barriers to realize UFG metallic materials, especially UFG steels, in large scale industries, since severe plastic deformation processes usually need special techniques and equipment, and large deformation forces are required for heavy plastic deformations. Cyclic heat treatments to repeat martensitic transformation and austenitization have been known as a simple way to fabricate fine-grained austenitic structures in steels. In the present study, we tried to make final ferrite microstructures ultrafine in a low-C steel by means of the cyclic heat treatment. Evolution of microstructures during the cyclic heat treatment was systematically investigated, putting stress on the change of grain sizes of austenite and ferrite. The austenite grain size decreased with increasing the number of heat treatment cycles, and the minimum average austenite grain size obtained was 11 μm. By having furnace-cooling from austenite states with various grain sizes, ferrite microstructures with different mean grain sizes were fabricated. We could successfully obtain a fine-grained ferrite structure with a mean grain size of 4.5 μm and nearly a random texture through the heat treatment without deformation. Microstructural features and mechanical properties of the obtained fine-grained ferritic structures were investigated by scanning electron microscope/electron back-scattering diffraction measurements and a tensile test at room temperature. The specimens with ferrite + pearlite microstructure with the smallest average ferrite grain size of 4.5 μm managed both high strength (yield strength of 375 MPa and tensile strength of 500 MPa) and large tensile ductility (uniform elongation of 20% and total elongation of 39%) in the simple 2Mn-0.1C steel.
Oral Anticoagulation and Risk of Symptomatic Hemorrhagic Transformation in Stroke Patients Treated With Mechanical Thrombectomy: Data From the Nordictus Registry
Authors: Ramos-Araque María E. , Chavarría-Miranda Alba , Gómez-Vicente Beatriz , López-Cancio Martínez Elena , Castañón Apilánez María , Castellanos Mar , López Fernández María , Tejada Meza Herbert , Marta Moreno Javier , Tejada García Javier , Beltrán Rodríguez Iria , de la Riva Patricia , Díez Noemi , Arias Rivas Susana , Santamaría Cadavid María , Bravo Anguiano Yolanda , Bártulos Iglesias Mónica , Palacio Portilla Enrique Jesús , Revilla García Marian , Timiraos Fernández Juan José , Arenaza Basterrechea Naroa , Maciñeiras Montero José Luis , Vicente Alba Pablo , Julián Villaverde Francisco José , Pinedo Brochado Ana , Azkune Itxaso , Mar Freijo M. , Luna Alain , Arenillas Juan F. 
Published: November 2020

Introduction: We aimed to evaluate if prior oral anticoagulation (OAC) and its type determines a greater risk of symptomatic hemorrhagic transformation in patients with acute ischemic stroke (AIS) subjected to mechanical thrombectomy.Materials and Methods: Consecutive patients with AIS included in the prospective reperfusion registry NORDICTUS, a network of tertiary stroke centers in Northern Spain, from January 2017 to December 2019 were included. Prior use of oral anticoagulants, baseline variables, and international normalized ratio (INR) on admission were recorded. Symptomatic intracranial hemorrhage (sICH) was the primary outcome measure. Secondary outcome was the relation between INR and sICH, and we evaluated mortality and functional outcome at 3 months by modified Rankin scale. We compared patients with and without previous OAC and also considered the type of oral anticoagulants.Results: About 1.455 AIS patients were included, of whom 274 (19%) were on OAC, 193 (70%) on vitamin K antagonists (VKA), and 81 (30%) on direct oral anticoagulants (DOACs). Anticoagulated patients were older and had more comorbidities. Eighty-one (5.6%) developed sICH, which was more frequent in the VKA group, but not in DOAC group. OAC with VKA emerged as a predictor of sICH in a multivariate regression model (OR, 1.89 [95% CI, 1.01–3.51], p = 0.04) and was not related to INR level on admission. Prior VKA use was not associated with worse outcome in the multivariate regression model nor with mortality at 3 months.Conclusions: OAC with VKA, but not with DOACs, was an independent predictor of sICH after mechanical thrombectomy. This excess risk was associated neither with INR value by the time thrombectomy was performed, nor with a worse functional outcome or mortality at 3 months.
Outcomes for Surgery in Stage IA Large Cell Lung Neuroendocrine Compared With Other Types of Non-Small Cell Lung Cancer: A Propensity Score Matching Study Based on the Surveillance, Epidemiology, and End Results (SEER) Database
Authors: Zou Liqing , Guo Tiantian , Ye Luxi , Zhou Yue , Chu Li , Chu Xiao , Ni Jianjiao , Zhu Zhengfei , Yang Xi 
Published: November 2020

BackgroundPulmonary large cell neuroendocrine cancer (LCNEC) is commonly classified as non-small cell lung cancer (NSCLC). Even for stage I disease, after surgery the survival is always poor, but clinical research on LCNEC is scant and always with unsatisfying sample sizes. Thus, we conduct the first study using the Surveillance, Epidemiology, and End Results (SEER) database to compare survival after surgery between stage I LCNEC and other types of NSCLC.MethodsFrom 2004 to 2016, 473 patients with stage IA LCNEC, 17,669 patients with lung adenocarcinoma (LADC) and 8,475 patients with lung squamous cell cancer (LSCC), all treated with surgery were identified. In addition, 1:1 PSM was used, and overall (OS) and cancer-specific survival (CSS) between groups were compared.ResultsThe 5-year OS rates and CSS rates for LCNEC were 52.5% and 81.5%, respectively. Overall, both OS and CSS were significantly superior for stage IA LADC than LCNEC (for OS: HR 0.636, 95% CI 0.568-0.712; for CSS: HR 0.688, 95% CI 0.561–0.842, LCNEC as reference), while comparable for LSCC with LCNEC (for OS: HR 0.974, 95% CI 0.869–1.091; for CSS: HR 0.907, 95% CI 0.738–1.115). PSM generated 471 pairs when LCNEC was compared with LADC and both OS and CSS were significantly better in LADC than LCNEC (for OS: HR 0.580, 95% CI 0.491–0.686; for CSS: HR 0.602, 95% CI 0.446–0.814). Of note, for the subgroup of patients ≤ 65 years old, HRs for both OS and CSS were lower (for OS: HR 0.470; for CSS: HR 0.482). As for comparison between LCNEC and LSCC, PSM generated 470 pairs. Differently, only CSS was significantly superior in LSCC than LCNEC (HR 0.563, 95% CI 0.392–0.807), while OS was not. Further grouping by age showed only CSS between two groups for patients with age ≤ 65 years old was significantly different (P = 0.006).ConclusionsWe report the first survival comparison after surgery between stage IA LCNEC and other types of NSCLC by SEER database and PSM. Our results demonstrated after surgery, stage IA LCNEC was worse in survival, especially compared to LADC. Extra clinical care should be paid, especially for younger patients. More studies investigating adjuvant therapy are warranted.
Prevalence of Workaholism Among Egyptian Healthcare Workers With Assessment of Its Relation to Quality of Life, Mental Health and Burnout
Authors: Kasemy Zeinab A. , Abd-Ellatif Eman E. , Abdel Latif Asmaa A. , Bahgat Nadia M. , Shereda Hanaa Mohammad Abo , Shattla Safaa Ibrahim , Aboalizm Samira E. , Abd Elhy Asmaa Hamed , Allam Abeer R. , Ramadan Ahmed N. , Amer Hemat Mostafa , Ahmed Naglaa Abdelmawgoud , AlJifri Abobakr A. , El Dalatony Mervat M. 
Published: November 2020

Introduction: Work is a social double edged weapon activity that may have positive and negative effects on individual's quality of life and health.Objectives: To estimate workaholism prevalence and to determine its effects on quality of life, mental health, and burnout among healthcare workers (HCWs).Methods: Using a cross-sectional study, 1,080 Egyptian participants distributed as HCWs and non-HCWs were recruited. The study included 4 questionnaires to assess workaholism, quality of life (QoL), Psychological capital questionnaire (PCQ), and General health questionnaire (GHQ). Maslach Burnout Inventory (MBI) was applied to critical specialty HCWs in addition to pro-inflammatory markers including Il6, TNFα, and CoQ10.Results: This study revealed that 24.4 and 24.8% of HCWs were workaholic and hardworking, respectively, in comparison to 5.9 and 28.1% among non-HCWs (P &lt; 0.001). Somatic symptoms and anxiety/ insomnia domains of GHQ were higher among HCWs than non-HCWs (P &lt; 0.001 and 0.002, respectively). QoL was significantly lower among HCWs than non-HCWs (P &lt; 0.001). Workaholism was reported among 43.2% of HCWs with critical specialty (P &lt; 0.001). Components of PCQ components were significantly higher among HCWs with critical specialty than non-critical HCWs while QoL showed the reverse (P &lt; 0.05). Working excessively was a predictor to burnout [Emotional exhaustion (β = –0.23) and depersonalization (β = −0.25)] and TNFα (β = 0.41). Emotional exhaustion was a predictor to Il6 (β = 0.66), TNFα (β = 0.73), and CoQ10 (β = −0.78).Conclusion: There is a significant association between workaholism and psychologically poor-health and poor quality of life among HCWs. Critical specialty healthcare workers showed association between workaholism, burnout and pro-inflammatory markers. Addressing of personal characteristics, supporting factors in the work environment and periodic examination of the healthcare workers and responding accordingly is required.
Metaanálisis de la frecuencia de JAK2 en mielofibrosis primaria según la técnica de detección
Authors: Mejía-Ochoa Mónica , Acevedo-Toro Paola Andrea , Cardona Arias Jaiberth Antonio 
Published: November 2020

Antecedentes y objetivo: en la literatura científica mundial la frecuencia de JAK2 presenta una alta heterogeneidad en las neoplasias mieloproliferativas crónicas. El objetivo de esta investigación fue analizar la prevalencia de la mutación JAK2 en mielofibrosis primaria (MFP) y compararla según la técnica de detección en los años 2007-2018.
Material y métodos: revisión sistemática con metaanálisis, usando 21 búsquedas en tres bases de datos multidisciplinarias. Se aplicaron las fases de identificación, tamización, elección e inclusión de la guía PRISMA. Se garantizó reproducibilidad y evaluación de la calidad metodológica. Los análisis se basaron en frecuencias y metaanálisis para la prevalencia de la mutación con su intervalo de confianza de 95%.
Resultados: se incluyeron 29 estudios con 744 pacientes, los cuales provienen principalmente de Corea, Brasil y China. La técnica más empleada fue AS-PCR, las prevalencias de JAK2 con esta técnica oscilaron entre 33.3 y 71.4%; con PCR en tiempo real entre 42.9 y 77.3%, con secuenciación de 14.3-57.4% y en ARMS de 36.4-83.3%. La prevalencia de JAK2 no presentó diferencias estadísticamente significativas según el tipo de prueba diagnóstica utilizada.
Conclusión: se evidencian altas frecuencias de la mutación JAK2V617F en MFP, lo que evidencia que el diagnóstico de esta entidad no debe realizarse únicamente por características clínicas y hematológicas sino también por la tamización genética de los pacientes.
Tuberculosis faríngea
Authors: Neyra León Jesús Andree , Gerecz Cornejo Enike Liliana Margit , Cabrejos Valderrama Janish Alberto Mao , Antonio Travezan Pedro Salvador 
Published: November 2020

La tuberculosis faríngea es una forma extrapulmonar de esta enfermedad que corresponde a menos de 1% de casos, mayormente de carácter secundario. En Perú, no hay datos de reportes de casos en esta forma de tuberculosis extrapulmonar.
Presentamos el caso de un varón de cuarenta y dos años que consultó por dolor de garganta y tumoración en faringe de dos meses de evolución, sin antecedentes epidemiológico o patológico. Se realiza biopsia de la lesión y coloración Ziehl Neelsen dando compatible a tuberculosis faríngea.
En la radiografía de tórax se encontró lesiones pulmonares diseminadas bilaterales de tipo miliar, confirmando forma secundaria a tuberculosis diseminada. Por ello, se resalta la importancia de descartar tuberculosis ante toda faringitis crónica sin respuesta al tratamiento convencional
Formación Didáctica L2 (Kaqchikel) en la ENBI Nim Na’oj
Authors: Chalí­ Sarech Lilian Esterlina 
Published: November 2020

Este artí­culo presenta los resultados de la investigación titulada ”Formación didáctica L2 (kaqchikel) en la ENBI Nim Na’oj”, realizada en enero y febrero de 2020.
La investigación tuvo como objetivo establecer las estrategias de enseñanza-aprendizaje que utiliza el docente en la formación didáctica que reciben los estudiantes de Magisterio Infantil Bilingüe Intercultural, para el abordaje la Segunda Lengua - L2 (kaqchikel). Los resultados muestran que tanto los documentos así­ también el docente no cuenta con una metodologí­a definida y técnicas adecuadas para el desarrollo de los contenidos didácticos propuestos en las sub-áreas didáctica L2.
Ante esta situación, existe la necesidad de actualización docente en el uso y domino de las cuatro competencias lingüí­sticas del Kaqchikel - L2 para desarrollo del curso de Didáctica, que facilite la interacción entre los estudiantes y docentes. El principal desafí­o de la Escuela Normal de Educación Bilingüe Intercultural Nim Na’oj será promover una educación de calidad que responda a las caracterí­sticas culturales de las comunidades indí­genas para asegurar el derecho de ser educados en su propia cultura en dos o más idiomas.
Le chartisme
Authors: Chase Malcolm 
Published: November 2020

De 1838 à 1858, en Grande-Bretagne, dans la première société industrielle, le chartisme fut un mouvement ouvrier inédit, mobilisant, à son apogée, trois millions d’hommes et de femmes. Les militants de la Charte du Peuple luttaient pour le suffrage universel et une société démocratique qui permettent à chacun de vivre dignement de son travail. À travers des pétitions de masse adressées au Parlement, des rassemblements et des manifestations, des journaux, des chants et des communautés villageoises, les chartistes envisageaient une autre société. Le chartisme étudie le mouvement dans son intégralité, en Grande-Bretagne et en Irlande. S’appuyant sur une grande maîtrise des archives et des publications d’époque, l’auteur s’est particulièrement intéressé à l’expérience des hommes, des femmes et des enfants du mouvement. Le récit haletant est entrecoupé de courtes « vies chartistes », qui constituent autant de passionnantes biographies. Cet ouvrage est, depuis 1913, le premier en français sur le sujet.
La genèse de l’expédition franco-britannique de 1956 en Égypte
Authors: Bernard Jean-Yves 
Published: November 2020

Au confluent de la guerre froide, de la décolonisation et du conflit israélo-arabe, la crise de Suez de 1956 tient une place particulière dans l’histoire des relations internationales depuis 1945. À ses acteurs principaux, Grande- Bretagne et France, associés dans une intervention militaire contre l’Égypte, l’historiographie a invariablement assigné des mobiles de politique étrangère ou coloniale : les Anglais voulaient préserver leurs positions dans le monde arabe et protéger leurs intérêts pétroliers ; les Français désiraient renverser Nasser, soutien essentiel des rebelles algériens.

S’agit-il donc, dans le cadre classique d’un affrontement entre nationalisme égyptien et impérialismes anglais et français, d’un retour étonnant et anachronique à la politique de la canonnière ? L’auteur démontre que les choix des deux gouvernements trouvent en réalité leurs raisons dans la politique intérieure au sens large, ressac conservateur de l’après-guerre en Grande-Bretagne et crise de la IVe République en France. Son étude porte à la fois sur les développements internes dans les deux pays à partir de 1955 et sur leurs politiques égyptiennes, sans négliger les plans militaires et les considérations coloniales. Outre les abondantes sources secondaires, Jean-Yves Bernard utilise l’ensemble des archives publiques françaises et anglaises disponibles, les fonds des partis politiques, les archives personnelles et les témoignages oraux de plusieurs acteurs et contemporains des faits.