bluuebunny/crossref_metadata_2025_split huggingface dataset:
Phytochemical Screening and Evaluation of the Anxiolytic and Cytotoxic Potential of the Leaves and Stem of Ocimum basilicum Linn. var. pilosum (Willd.) Benth.
Authors: Didari Sayeda Saima , Maria Nazratun Noor , Afrin Syeda Rubaiya , Jannat Sarah Waddun , Islam Mohammad Rashedul , Hossain Mohammed Kamrul 
Published: December 2024

This research investigated the qualitative screening of phytochemicals, anxiolytic and cytotoxic potentials of crude methanol extracts of the leaves (MEL) and stem (MES) of Ocimum basilicum Linn. var. pilosum (Willd.) Benth is a new variant of sweet basil herb. For the solvent extraction process, methanol was selected due to its ability to solubilize both polar and non-polar plant constituents. Phytochemical analysis was carried out by using the established methods. The anxiolytic activity was assessed on Swiss albino mice using the hole board apparatus, and cytotoxicity was measured using the brine shrimp lethality assay. The current results showed that MEL and MES contained several active secondary metabolites, including alkaloids, glycosides, flavonoids, phenols, tannins, triterpenes, and resins. MEL (200, 400 mg/kg) and MES (400 mg/kg) were found to be significantly (P < 0.001) effective anxiolytic agents as compared to the negative control group. In the case of cytotoxicity activity, MEL showed an LC50 value of 244.45 µg/ml (standard 0.16 µg/ml), indicating cytotoxic potential compared to the negative control. Thus, the findings revealed that MEL and MES were excellent anxiolytic agents with a moderate cytotoxic potential.
Chittagong Univ. J. B. Sci. Vol. 12 (1 & 2): 27-34, 2024
Study on Microbial and Ecophysiological Indices of Tea Soils in Chattogram and Khagrachari Districts of Bangladesh
Authors: Akter Sanjida , Das Shuva , Roy Sajal , Chowdhury Nasrin 
Published: December 2024

The evaluation of soil microbial and ecophysiological properties is highly significant to maintain soil health and sustainable production of crops. Thus, this research was carried out to observe the status of different microbial and ecophysiological indices of tea soils belonging to Oodaleah tea garden (OTG) and Tin Tohori tea garden (TTTG) located in Chattogram and Khagrachari districts of Bangladesh. A total number of thirty seven and twenty four surface soil (0-9 cm) samples were collected from OTG and TTTG, respectively. The areas of both the tea gardens are mostly covered by low hill ranges to terraces. Irrespective of the sampling sites, the mean values of microbial biomass carbon (MBC), microbial activity (MA), substrate induced respiration (SIR), microbial quotient (qMic) and mineralization quotient (qM) were found to be 3.73%, 6.25%, 2.15%, 26.19% and 20.0% higher, while metabolic quotient (qCO2) was 7.69% lower in TTTG compared to OTG. The findings of this study clearly showed the highest microbial responses at toeslope except MA in TTTG. As for OTG, the MA and qM at toeslope significantly differed (p<0.05) from the values found at summit. However, all the microbial and ecophysiological indices did not differ significantly (p>0.05) among the three different slopes for TTTG. All the microbial indices were positively correlated, while a significant (p<0.01) negative correlation was found between MBC and qCO2 both for OTG and TTTG. Besides, the correlation between qCO2 and qMic was also found negative and significant (p<0.05) both for OTG and TTTG.
Chittagong Univ. J. B. Sci. Vol. 12 (1 & 2): 45-55, 2024
Decolorization of Reactive Dyes by Bacteria Isolated from Textile Dye-Contaminated Soil
Authors: Bhuiyan A R , Al Masud H M Abdullah , Hossain Md Towhid 
Published: December 2024

Textile dye-contaminated soil samples were collected for the isolation of heterotrophic bacterial population. The isolates were screened primarily for dye decolorization using Luria Bertani broth containing 100 mgL-1 of selected reactive dyes namely Blue FNR (BF), Novacron Navy (NN), or Novacron Deep Cherry (NDC). Secondary screening of dye-decolorizing bacterial isolates was carried out in Bushnell Haas medium. The decolorization efficiency of the isolates was estimated spectrophotometrically. The selected isolates were identified by the analysis of their cultural, biochemical, and molecular characteristics. Among 38 bacterial isolates from the dye-contaminated soil, two bacterial genera Klebsiella pneumoniae strain OS-3 and Bacillus tropicus strain OS-8 were found to have strong ability in selected dye-decolorization. The optimum pH and temperature for dye decolorization in static conditions by both isolates were found to be 6-8 and 37°C within 72 hours, respectively. Efficient dye decolorization was observed under static condition in compared to shaking culture. Both isolates were able to induce decolorization in the presence of co-substrate. Starch, sucrose and glucose as a carbon source and yeast extract as an organic nitrogen source induced the efficiency of Blue FNR, Novacron Navy and NDC dyes decolorization, respectively by both isolates. On the other hand, it was observed that the percentage of dye degradation decreased in the presence of inorganic nitrogen source in the medium. Both isolates retained efficiency to decolorize selected reactive dyes by 70-80% at 5% salinity. The strong decolorizing ability in natural conditions indicates that both bacteria could be used in dye-polluted wastewater treatment.
Chittagong Univ. J. B. Sci. Vol. 12 (1 & 2): 91-102, 2024
Evaluation of Bacterial Population in Drinking Water Along with Hygiene and Sanitation Facilities of Different Schools in Port City, Bangladesh
Authors: Alam Md Zobaidul , Dey Shampa Priya 
Published: December 2024

In our daily life, drinking water is one of the most essential elements. However, safe and good-quality drinking water is a major concern for human health. Therefore, our study assessed the sanitation, hygiene conditions, and evaluation of bacterial population of drinking water in 40 schools in the port city, of Bangladesh. Total viable count (TVC), total coliform count (TCC), and survey questionnaire were used to complete our study. About 65% of water samples exceeded the acceptable limit (>500 cfu/ml) detected by TVC. The most probable number (MPN) method was used to see the presence of coliform bacteria where about 65% of samples were found contaminated with coliform, among them 12.5% of samples were at very high risk according to World Health Organization (WHO) guidelines. A total of 10 bacterial isolates like Enterobacter sp., E. coli, Klebsiella sp., Salmonella sp., Proteus sp., Serratia sp., Citrobacter sp., Pseudomonas sp., Alcaligenes sp., and Micrococcus sp. were detected in water samples by using four selective media and then identified by using cultural, morphological and biochemical tests. The disc diffusion method was used to detect antibiotic sensitivity with 10 commercially available antibiotics. About 80% of isolates were found completely resistant to erythromycin, amoxicillin, ampicillin, and rifampin and 90% were sensitive to ciprofloxacin, gentamicin, azithromycin, and tetracycline. Antibiotic resistance isolates might pose a serious threat to human health. In most schools, reservoirs of drinking water were found contaminated by various bacteria. Only 48.3% of schools used separate toilets for girls but of poor-quality hygiene and sanitation facilities. Due to poor hygiene, sanitation, and contaminated drinking water, students may suffer from various diseases. Therefore, this study emphasizes the need to raise awareness about hygiene and sanitation facilities by regularly monitoring school drinking water quality to ensure children are out of waterborne diseases.
Chittagong Univ. J. B. Sci. Vol. 12 (1 & 2): 130-142, 2024
Prescription Pattern of Antihypertensive Drugs in Chronic Kidney Disease Patients Attending - A Tertiary Care Hospital
Authors: Parvin Shahina , Akter Nargis , Rizvi HM Mustafizur Rahman , Afroz Rozina , Biswas Ripa , Sultana Masuma , Nasrin Shamima , Hossain Abul , Sultana Papiya 
Published: December 2024

Background: Chronic kidney disease exists both as a common cause of hypertension and complication of uncontrolled hypertension. This complex interplay of hypertension and CKD further increases the risk of adverse cardiovascular and cerebrovascular outcomes. Blood pressure control is pertinent in all stages of chronic kidney disease.
Method: An observational descriptive cross-sectional study was conducted in Nephrology Department of Dhaka Medical College Hospital from January 2022 to December 2022. Total 264 hypertensive CKD patients were included in this study according to selection criteria. Collected data were processed and analyzed by using SPSS 26.0. p value of < 0.05 was taken as statistically significant.
Result: Among 264 CKD patients 58% male, 42% female and mean age was 49.43±13.87years. Only 67% patient achieved control of BP with antihypertensive. 53.8% patient were in stage-5 and 18.2% in stage-4 CKD. 85.6% patient received combination therapy. Among them 58.3% patients received two drug combination therapy. CCBs was prescribed in 169 patients. Amlodipine was 41.42% the preferred CCB and the combination of ARBs+CCBs was (28.7%) the frequently prescribed. Control of blood pressure was achieved in CKD patients taking monotherapy (p = 0.039) and on three drug combination therapy (p = 0.006).
Conclusion: Calcium channel blocker is the treatment of choice in CKD patients with hypertension and commonly prescribed CCB is amlodipine. Combination of antihypertensive therapy is a better choice for control of hypertension.
J Com Med Col Teachers Asso July 2024; 28(2): 70-74
Comparative Study between Exatracorporeal Shock Wave Lithotripsy (ESWL) and Percutaneours Nephrolithotomy (PCNL) for the Treatment of Lower Calyceal Stone
Authors: Amin Mohammad Ruhul , Ullah Mohammad Shafayet , Alam Mohammad Mahbubul Alam , Majumder Sharif Muhammad Mahmudul Hasan , Hasan Mohammad Masum , Begum Taslima 
Published: December 2024

Background: The main question when comparing ESWL and PCNL for lower pole stones is one of clinical effectiveness, which includes consideration of the stone-free rate, secondary procedure rate, complications, hospitalization, disability period and recurrent stone rate. Many factors are contributed to lower pole stones clearance such as size of stone, composition, infundibulo-pelvic angle and patient clinical factors should all be considered in conjunction with the various surgical modalities and the availability of equipment’s before the preferred treatment option is selected. Stone-free rate with ESWL were inversely correlated to the stone burden treated. The introduction of ESWL in the early 1980s temporarily limited the indications for percutaneous procedures for stones but after limitations of shock wave lithotripsy were realized, percutaneous surgery regained its merited role in the stone management. On the other hand, lower pole stones contraindication for ESWL which were largely treated by open surgery in the past, now mostly replaced by PCNL.
Objectives: To assess and compare relative efficacy and the overall lower calyceal stone clearance in between Percutaneous Nephrolithotomy (PCNL) and Extracorporeal Shock Wave Lithotripsy (ESWL). In relation to stone size. To compare the anatomical factor infundibulo-pelvic angle among, required adjuvant procedure and morbidity in between PCNL and ESWL in treating lower calyceal.
Study Design: Hospital based prospective comparative interventional study. Setting: Study was carried out in the Department of urology, Bangabandhu Sheikh Mujib Medical University (BSMMU), and different private hospital of Dhaka city, during the period of January 2006 to July 2007.
Methods: Purposive sampling method was followed as per inclusion and exclusion by criteria to select 60 patients. They were divided into two groups by alternate method, into PCNL and ESWL groups.
Result: In this study, for the smaller stones <10mm, it was seen that the rate of stone clearance in PCNL and ESWL group were 85.71% and 77.77% respectively. Statistical analysis shows no significant difference of stone clearance between the two groups. (P>0.05). For treating larger stones >10-20mm, present study showed that the rate of stone clearance in PCNL and ESWL group were 93.75% and 41.66 % respectively. There are statistical analysis shows significant difference of clearance (P<0.05).
Conclusion: Percutaneous Nephrolithotomy (PCNL) is better option than Extracorporeal Shock Wave Lithotripsy (ESWL) in treating lower calyceal stone.
J Com Med Col Teachers Asso July 2024; 28(2): 92-95
HCV infection in haemodialysis patients: Prevalence and risk factor analysis
Authors: Safwath Sayed Alamgir , Kabir Iqbal Murshed , Mahmuduzzaman Muhammad , Gosh Dilip Kumar , Ahmed Mostak Uddin , Mamoon Md  Abdullah Al , Rahman Md  Anisur 
Published: December 2024

Introduction: Dialysis patients have a higher rate of HCV infection.2 This study was carried out to find out the prevalence of HCV infection in patients on chronic haemodialysis and to identify the risk factors for HCV transmission in this patient group. Methods: This prospective cross sectional study was carried out in the haemodialysis units and the department of Gastrointestinal, Hepatobiliary and pancreatic disorders (GHPD) of BIRDEM hospital, during the period of March 2006 to March 2007. A total of 72 end stage renal disease patients of both sexes and all ages on maintenance heamodialysis for more than three months were enrolled. A pre-designed questionnaire comprising demographic, dialysis-specific, medical history and life style variables was filled up. Predialysis five (5) ml blood were taken from the arterial channel for anti-HCV (ELISA) test. P-value <0.05 was considered as significant. Results: The prevalence of HCV infection in patients on chronic haemodialysis was 23.6%. There was no statistical significance (p=0.133ns) between anti-HCV positivity and age and sex distribution. The association between HCV status and the total number of units of blood transfused, mean duration of haemodialysis and life-style risk factors for HCV transmission were statistically significant. Conclusion: In our study, the prevalence of HCV infection in patients on chronic haemodialysis was 23.6%. The haemodialysis patients in our dialysis unit have an infection rate 8-12 fold more than general population. The association between the total number of units of blood transfused, mean duration of haemodialysis, and presence of one or more risk factors and HCV positivity were statistically significant.
J. Dhaka National Med. Coll. Hos. 2014;20(01): 9-14
Prescribing Pattern of Antibiotic usage for Urinary Tract Infection in two tertiary care hospital
Authors: Khatun Shima Akter , Islam Md Rashidul , Saha Shusmita , Ara Ferdous 
Published: December 2024

Urinary tract infection is the most common infectious disease in the community medical practice. It was a cross-sectional descriptive study conducted on patients diagnosed with UTI. The objective was to study the prescribing pattern of antibiotic used in the treatment of UTI. The study was carried out in the OPD of Medicine, Surgery and Gynaecology & Obstetrics from 1st January to 31st March 2012 in Sir Salimullah Medical College and Mitford Hospital and Dhaka National Medical College Hospital, Dhaka. Urinary Tract Infection patients who were in the age group of 18-80 years were included after obtaining their informed consent. A suitable data collection form was prepared and used to collect the required data. Among 360 patients 180 from Sir Salimullah Medical College and 180 from Dhaka National Medical college. Most of the patients were prescribed with Ceftriaxone, Cefotaxime and ciprofloxacin respectively in Sir Salimullah Medical college and cefotaxime, nitrofurantoin and ciprofloxacin respectively in Dhaka National Medical College. The study found that Cephalosporin’s were most commonly used and Quinolones were the second most commonly used drugs for the treatment of UTI. The third category drug used in female patients was Nitrofurantoin which is relatively inexpensive and safe for both pregnant and non-pregnant women.
J. Dhaka National Med. Coll. Hos. 2014;20(01):25-29
Comparison of Hybrid Capture II (HCII) assay with conventional methods for diagnosis of Human Papilloma Virus (HPV) infection
Authors: Rahman Tanjila , Tabassum Shahina , Saha Shimu , Jahan Munira 
Published: December 2024

Human papilloma virus (HPV) is the most prevalent virus involved in sexually transmitted diseases worldwide, and an important public health challenge for the prevention of cervical carcinoma. The present study compared the hybrid capture II assay with conventional methods for the diagnosis of HPV infection. A total of 68 women between 25-70 years of age were included in this study. Conventional methods used for diagnosis of the patients included Visible Inspection of Acetic acid (VIA), Histopathology and Pap smear tests. The hybrid Capture 2 assay was used to detect HPV DNA viral load in these patients. VIA test was performed on 66 patients. HPV DNA was detected in 8 (20.5%) of the VIA positive cases, 2 (9.5%) VIA negative, and 2 (33.3%) cases where VIA test were unsatisfactory. Pap smear test was done on 43 patients. Among them HPV DNA was detected in 13(19.1%) cases, of which 11 (37.9%) cases were Pap positive/abnormal and 2 (14.3%) were Pap negative. Out of 29 cases where histopathological examination was carried out, HPV DNA was detected in 1 (7.7%) chronic cervicitis case, 4 (66.7%) cases of CIN I, 7 (70.0%) cases of CIN II/III and invasive carcinoma. From this study it is suggested that in combination with conventional methods, hybrid Capture 2 assay is a useful tool to diagnose high-risk HPV infections when conventional tests shows apparently normal results.
J. Dhaka National Med. Coll. Hos. 2014;20(01):30-34
Relaparotomy in Obstetrics and Gynaecology Department of a Medical College Hospital in Old Dhaka City
Authors: Sultana Nasreen , Rahman Jahanara , Begum Hosne Ara , Nahar Najmun , Jesmin Asma 
Published: December 2024

The objectives of this study were to analyze the cases that underwent relaparotomy and identify indications of primary and relaparotomy surgeries, relaparotomy findings, and procedures undertaken and find out avoidable risk factors of such emergency situations. This was a retrospective descriptive study done in Dhaka National Medical College Hospital during the period from July 2011 to June 2013. Out of 6983 operations (Obstetric and Gynae) 13 cases needed relaparotomy. In ten case primary operations were performed in this hospital and three were performed in outside clinics( one outside Dhaka).Indications of relaparotomy were, internal haemorrhage, foreign body, rectus sheath haematoma, secondary PPH, fecal fistula, post surgery adhesion. Procedure performed during repeat operations were hysterectomy, resuturing of uterine incision ,peritoneal toileting and removal of blood clot, removal of foreign body, drainage of rectus sheath haematoma, fecal fistula repair, adhesiolysis. To reduce the risk of relaparotomy and to minimize the sufferings of the patients from relaparotomy, proper case selection during primary operation, competency of the surgical team, appropriate surgical procedures, prompt identification of complication and early necessary intervention should be maintained in every hospital.
J. Dhaka National Med. Coll. Hos. 2014;20(01):47-49
Green Management Framework Early Implementation With a Pentuple Bottom Line Approach for IKM in Malang City
Authors: Purnomo Purnomo , Wicaksono Soetam Rizky , Setiawan Rudy 
Published: December 2024

In the era of free trade encourage the Indonesian government's trade policy which leads to an increase in the high competitiveness of these products/services, Small and Medium Industries (Industri Kecil Menengah = IKM). The need for industry in IKM to have a sustainable manufacturing process is a must that must be met, considering the five pillars referred to as pentuples, namely profit, planet, people, phenotechnology, and prophet. Especially in the city of Malang, East Java Indonesia, has difficulties in terms of waste disposal and raw material management in general. Framework is needed that is able to connect the application of green management for the efficiency of the manufacturing process in IKMs by adapting existing local cultures. When they were offered an introduction to Green Management, the response they got was a fairly good, however, some obstacles occur when they try to implement good Green Management as their company strategy. The use of Green management in the management of IKM is basically acceptable to IKM entrepreneurs in the city of Malang. In its use, it can be supported by a pentuple implementation strategy that involves five components in it. So that in the end it can go towards sustainable manufacturing for IKM, especially in the city of Malang. However, when explained about the generic pentuple component, entrepreneurs can be triggered by their motivation to implement Green management through the Prophet component which contains spiritual elements. This is because the scope of entrepreneurs tends to be religious and the majority are Moslem, so it is easier to influence through this aspect.
Robust Regression Analysis Of Gm Estimation On The Poverty Gap Index Of Indonesian Provinces
Authors: Nabil Fauzaan , Susanti Yuliana , Zukhronah Etik 
Published: December 2024

Poverty has been a severe problem in Indonesia since the post-independence era until today. One indicator that can be used to measure the poverty level in a region is the poverty gap index, which describes the average size of the gap between each population and the poverty line. The data on the poverty gap index in Indonesia in 2022 contains outliers and misleading data that are not normally distributed, so the least squares method is inappropriate. One method that can be used to overcome the outlier problem is robust regression analysis. This study aims to determine the Generalized M (GM) estimation robust regression model and the factors that affect the poverty gap index in provinces in Indonesia. The estimation used is GM estimation, the development of M estimation when M estimation is less sensitive to outliers. The results showed that the GM estimation robust regression model has a value of 100% It was also found that the factors that significantly affect the poverty gap index in Indonesia in 2022 are the percentage of poor people, the Gini ratio, the poverty line, and the percentage of households with a PLN electricity source.
National Center for Biotechnology Information (NCBI) database for Pseudomonas aeruginosa
Authors: Giyanto  , Julianti Elisa , Sinaga Hotnida , Sembiring Mariani 
Published: December 2024

The present study describes search National Centre for Biotechnology Information (NCBI) databases (GQuery) on Pseudomonas aeruginosa. NCBI databases search (https://www.ncbi.nlm.nih.gov/) was employed to generate a number of valuable information. Results found in 31 databases for Pseudomonas aeruginosa. All databases of Pseudomonas aeruginosa consist of literature, genes, protein, genomes, clinical and pubchem. The literature contained bookshelf, MeSH (Medical Subject Headings), National Library of Medicine Catalogue, PubMed, and PubMed Central. The genes consists of gene, geo datasets,gep profiles, popset. Proteins feature contained conserved domains, identical protein groups, protein, protein family models and structure. Genomes included Assembly, BioProject, BioSample, Genome, Nucleotide, SRA, and Taxonomy. The clinical contained clinicaltrials.gov, clinvar, dbgap, gtr, medgen and imim. The pubchem consist of bioassays, compunds, pathways, substances. The present work provides essential information relating to biotechnology of Pseudomonas aeruginosa.