bluuebunny/crossref_metadata_2025_split huggingface dataset:
A general method for the creation of dilational surfaces
Authors: Broeren Freek G. J. , van de Sande Werner W. P. J. , van der Wijk Volkert , Herder Just L. 
Published: November 2019

AbstractDilational structures can change in size without changing their shape. Current dilational designs are only suitable for specific shapes or curvatures and often require parts of the structure to move perpendicular to the dilational surface, thereby occupying part of the enclosed volume. Here, we present a general method for creating dilational structures from arbitrary surfaces (2-manifolds with or without boundary), where all motions are tangent to the described surface. The method consists of triangulating the target curved surface and replacing each of the triangular faces by pantograph mechanisms according to a tiling algorithm that avoids collisions between neighboring pantographs. Following this algorithm, any surface can be made to mechanically dilate and could, theoretically, scale from the fully expanded configuration down to a single point. We illustrate the method with three examples of increasing complexity and varying Gaussian curvature.
A therapeutic antibody targeting osteoprotegerin attenuates severe experimental pulmonary arterial hypertension
Authors: Arnold Nadine D. , Pickworth Josephine A. , West Laura E. , Dawson Sarah , Carvalho Joana A. , Casbolt Helen , Braithwaite Adam T. , Iremonger James , Renshall Lewis , Germaschewski Volker , McCourt Matthew , Bland-Ward Philip , Kowash Hager , Hameed Abdul G. , Rothman Alexander M. K. , Frid Maria G. , Roger Thompson A. A. , Evans Holly R. , Southwood Mark , Morrell Nicholas W. , Crossman David C. , Whyte Moira K. B. , Stenmark Kurt R. , Newman Christopher M. , Kiely David G. , Francis Sheila E. , Lawrie Allan 
Published: November 2019

AbstractPulmonary arterial hypertension (PAH) is a rare but fatal disease. Current treatments increase life expectancy but have limited impact on the progressive pulmonary vascular remodelling that drives PAH. Osteoprotegerin (OPG) is increased within serum and lesions of patients with idiopathic PAH and is a mitogen and migratory stimulus for pulmonary artery smooth muscle cells (PASMCs). Here, we report that the pro-proliferative and migratory phenotype in PASMCs stimulated with OPG is mediated via the Fas receptor and that treatment with a human antibody targeting OPG can attenuate pulmonary vascular remodelling associated with PAH in multiple rodent models of early and late treatment. We also demonstrate that the therapeutic efficacy of the anti-OPG antibody approach in the presence of standard of care vasodilator therapy is mediated by a reduction in pulmonary vascular remodelling. Targeting OPG with a therapeutic antibody is a potential treatment strategy in PAH.
Prolyl hydroxylase substrate adenylosuccinate lyase is an oncogenic driver in triple negative breast cancer
Authors: Zurlo Giada , Liu Xijuan , Takada Mamoru , Fan Cheng , Simon Jeremy M. , Ptacek Travis S. , Rodriguez Javier , von Kriegsheim Alex , Liu Juan , Locasale Jason W. , Robinson Adam , Zhang Jing , Holler Jessica M. , Kim Baek , Zikánová Marie , Bierau Jörgen , Xie Ling , Chen Xian , Li Mingjie , Perou Charles M. , Zhang Qing 
Published: November 2019

AbstractProtein hydroxylation affects protein stability, activity, and interactome, therefore contributing to various diseases including cancers. However, the transiency of the hydroxylation reaction hinders the identification of hydroxylase substrates. By developing an enzyme-substrate trapping strategy coupled with TAP-TAG or orthogonal GST- purification followed by mass spectrometry, we identify adenylosuccinate lyase (ADSL) as an EglN2 hydroxylase substrate in triple negative breast cancer (TNBC). ADSL expression is higher in TNBC than other breast cancer subtypes or normal breast tissues. ADSL knockout impairs TNBC cell proliferation and invasiveness in vitro and in vivo. An integrated transcriptomics and metabolomics analysis reveals that ADSL activates the oncogenic cMYC pathway by regulating cMYC protein level via a mechanism requiring ADSL proline 24 hydroxylation. Hydroxylation-proficient ADSL, by affecting adenosine levels, represses the expression of the long non-coding RNA MIR22HG, thus upregulating cMYC protein level. Our findings highlight the role of ADSL hydroxylation in controlling cMYC and TNBC tumorigenesis.
All WSe2 1T1R resistive RAM cell for future monolithic 3D embedded memory integration
Authors: Sivan Maheswari , Li Yida , Veluri Hasita , Zhao Yunshan , Tang Baoshan , Wang Xinghua , Zamburg Evgeny , Leong Jin Feng , Niu Jessie Xuhua , Chand Umesh , Thean Aaron Voon-Yew 
Published: November 2019

Abstract3D monolithic integration of logic and memory has been the most sought after solution to surpass the Von Neumann bottleneck, for which a low-temperature processed material system becomes inevitable. Two-dimensional materials, with their excellent electrical properties and low thermal budget are potential candidates. Here, we demonstrate a low-temperature hybrid co-integration of one-transistor-one-resistor memory cell, comprising a surface functionalized 2D WSe2p-FET, with a solution-processed WSe2 Resistive Random Access Memory. The employed plasma oxidation technique results in a low Schottky barrier height of 25 meV with a mobility of 230 cm2 V−1 s−1, leading to a 100x performance enhanced WSe2p-FET, while the defective WSe2 Resistive Random Access Memory exhibits a switching energy of 2.6 pJ per bit. Furthermore, guided by our device-circuit modelling, we propose vertically stacked channel FETs for high-density sub-0.01 μm2 memory cells, offering a new beyond-Si solution to enable 3-D embedded memories for future computing systems.
Oxygen-catalysed sequential singlet fission
Authors: Wollscheid Nikolaus , Pérez Lustres J. Luis , Kefer Oskar , Hahn Sebastian , Brosius Victor , Bunz Uwe H. F. , Motzkus Marcus , Buckup Tiago 
Published: November 2019

AbstractSinglet fission is the photoinduced conversion of a singlet exciton into two triplet states of half-energy. This multiplication mechanism has been successfully applied to improve the efficiency of single-junction solar cells in the visible spectral range. Here we show that singlet fission may also occur via a sequential mechanism, where the two triplet states are generated consecutively by exploiting oxygen as a catalyst. This sequential formation of carriers is demonstrated for two acene-like molecules in solution. First, energy transfer from the excited acene to triplet oxygen yields one triplet acene and singlet oxygen. In the second stage, singlet oxygen combines with a ground-state acene to complete singlet fission. This yields a second triplet molecule. The sequential mechanism accounts for approximately 40% of the triplet quantum yield in the studied molecules; this process occurs in dilute solutions and under atmospheric conditions, where the single-step SF mechanism is inactive.
Cohesin cleavage by separase is enhanced by a substrate motif distinct from the cleavage site
Authors: Rosen Laura E. , Klebba Joseph E. , Asfaha Jonathan B. , Ghent Chloe M. , Campbell Melody G. , Cheng Yifan , Morgan David O. 
Published: November 2019

AbstractChromosome segregation begins when the cysteine protease, separase, cleaves the Scc1 subunit of cohesin at the metaphase-to-anaphase transition. Separase is inhibited prior to metaphase by the tightly bound securin protein, which contains a pseudosubstrate motif that blocks the separase active site. To investigate separase substrate specificity and regulation, here we develop a system for producing recombinant, securin-free human separase. Using this enzyme, we identify an LPE motif on the Scc1 substrate that is distinct from the cleavage site and is required for rapid and specific substrate cleavage. Securin also contains a conserved LPE motif, and we provide evidence that this sequence blocks separase engagement of the Scc1 LPE motif. Our results suggest that rapid cohesin cleavage by separase requires a substrate docking interaction outside the active site. This interaction is blocked by securin, providing a second mechanism by which securin inhibits cohesin cleavage.
Rpd3L and Hda1 histone deacetylases facilitate repair of broken forks by promoting sister chromatid cohesion
Authors: Ortega Pedro , Gómez-González Belén , Aguilera Andrés 
Published: November 2019

AbstractGenome stability involves accurate replication and DNA repair. Broken replication forks, such as those encountering a nick, lead to double strand breaks (DSBs), which are preferentially repaired by sister-chromatid recombination (SCR). To decipher the role of chromatin in eukaryotic DSB repair, here we analyze a collection of yeast chromatin-modifying mutants using a previously developed system for the molecular analysis of repair of replication-born DSBs by SCR based on a mini-HO site. We confirm the candidates through FLP-based systems based on a mutated version of the FLP flipase that causes nicks on either the leading or lagging DNA strands. We demonstrate that Rpd3L and Hda1 histone deacetylase (HDAC) complexes contribute to the repair of replication-born DSBs by facilitating cohesin loading, with no effect on other types of homology-dependent repair, thus preventing genome instability. We conclude that histone deacetylation favors general sister chromatid cohesion as a necessary step in SCR.
Abiotic synthesis of graphite in hydrothermal vents
Authors: Estes Emily R. , Berti Debora , Coffey Nicole R. , Hochella Michael F. , Wozniak Andrew S. , Luther George W. 
Published: November 2019

AbstractDeciphering the origin, age, and composition of deep marine organic carbon remains a challenge in understanding the dynamics of the marine carbon cycle. In particular, the composition of aged organic carbon and what allows its persistence in the deep ocean and in sediment is unresolved. Here, we observe that both high and low temperature hydrothermal vents at the 9° 50′ N; 104° 17.5 W East Pacific Rise (EPR) vent field are a source for (sub)micron-sized graphite particles. We demonstrate that commonly applied analytical techniques for quantification of organic carbon detect graphite. These analyses thereby classify graphite as either dissolved or particulate organic carbon, depending on the particle size and filtration method, and overlook its relevance as a carbon source to the deep ocean. Settling velocity calculations indicate the potential for these (sub)micron particles to become entrained in the buoyant plume and distributed far from the vent fields. Thus, our observations provide direct evidence for hydrothermal vents acting as a source of old carbon to the deep ocean.
Neural representations of honesty predict future trust behavior
Authors: Bellucci Gabriele , Molter Felix , Park Soyoung Q. 
Published: November 2019

AbstractTheoretical accounts propose honesty as a central determinant of trustworthiness impressions and trusting behavior. However, behavioral and neural evidence on the relationships between honesty and trust is missing. Here, combining a novel paradigm that successfully induces trustworthiness impressions with functional MRI and multivariate analyses, we demonstrate that honesty-based trustworthiness is represented in the posterior cingulate cortex, dorsolateral prefrontal cortex and intraparietal sulcus. Crucially, brain signals in these regions predict individual trust in a subsequent social interaction with the same partner. Honesty recruited the ventromedial prefrontal cortex (VMPFC), and stronger functional connectivity between the VMPFC and temporoparietal junction during honesty encoding was associated with higher trust in the subsequent interaction. These results suggest that honesty signals in the VMPFC are integrated into trustworthiness beliefs to inform present and future social behaviors. These findings improve our understanding of the neural representations of an individual’s social character that guide behaviors during interpersonal interactions.
Locally collective hydrogen bonding isolates lead octahedra for white emission improvement
Authors: Cui Bin-Bin , Han Ying , Huang Bolong , Zhao Yizhou , Wu Xianxin , Liu Lang , Cao Guangyue , Du Qin , Liu Na , Zou Wei , Sun Mingzi , Wang Lin , Liu Xinfeng , Wang Jianpu , Zhou Huanping , Chen Qi 
Published: November 2019

AbstractAs one of next-generation semiconductors, hybrid halide perovskites with tailorable optoelectronic properties are promising for photovoltaics, lighting, and displaying. This tunability lies on variable crystal structures, wherein the spatial arrangement of halide octahedra is essential to determine the assembly behavior and materials properties. Herein, we report to manipulate their assembling behavior and crystal dimensionality by locally collective hydrogen bonding effects. Specifically, a unique urea-amide cation is employed to form corrugated 1D crystals by interacting with bromide atoms in lead octahedra via multiple hydrogen bonds. Further tuning the stoichiometry, cations are bonded with water molecules to create a larger spacer that isolates individual lead bromide octahedra. It leads to zero-dimension (0D) single crystals, which exhibit broadband ‘warm’ white emission with photoluminescence quantum efficiency 5 times higher than 1D counterpart. This work suggests a feasible strategy to modulate the connectivity of octahedra and consequent crystal dimensionality for the enhancement of their optoelectronic properties.
Behavioural plasticity and the transition to order in jackdaw flocks
Authors: Ling Hangjian , Mclvor Guillam E. , Westley Joseph , van der Vaart Kasper , Vaughan Richard T. , Thornton Alex , Ouellette Nicholas T. 
Published: November 2019

AbstractCollective behaviour is typically thought to arise from individuals following fixed interaction rules. The possibility that interaction rules may change under different circumstances has thus only rarely been investigated. Here we show that local interactions in flocks of wild jackdaws (Corvus monedula) vary drastically in different contexts, leading to distinct group-level properties. Jackdaws interact with a fixed number of neighbours (topological interactions) when traveling to roosts, but coordinate with neighbours based on spatial distance (metric interactions) during collective anti-predator mobbing events. Consequently, mobbing flocks exhibit a dramatic transition from disordered aggregations to ordered motion as group density increases, unlike transit flocks where order is independent of density. The relationship between group density and group order during this transition agrees well with a generic self-propelled particle model. Our results demonstrate plasticity in local interaction rules and have implications for both natural and artificial collective systems.
Menstrual cycle associated changes in hormone-related gene expression in oestrogen receptor positive breast cancer
Authors: Haynes Ben P. , Ginsburg Ophira , Gao Qiong , Folkerd Elizabeth , Afentakis Maria , Buus Richard , Quang Le Hong , Thi Han Pham , Khoa Pham Hong , Dinh Nguyen Van , To Ta Van , Clemons Mark , Holcombe Chris , Osborne Caroline , Evans Abigail , Skene Anthony , Sibbering Mark , Rogers Clare , Laws Siobhan , Noor Lubna , Smith Ian E. , Dowsett Mitch 
Published: November 2019

AbstractThe major changes in hormone levels that occur through the menstrual cycle have been postulated to affect the expression of hormone-regulated and proliferation-associated genes (PAGs) in premenopausal ER+ breast cancer. Whilst previous studies have demonstrated differences in gene expression, here, we investigated if there are within patient changes in the expression of oestrogen- and progesterone-regulated genes (ERGs and PRGs) and PAGs in ER+ breast cancer during the menstrual cycle. Samples from 96 patients in two independent prospective studies of the effect of menstrual cycle on ER+ breast cancer were used. Plasma hormone measurements were used to assign tumours to one of three pre-defined menstrual cycle windows: W1 (days 27–35 and 1–6; low oestradiol and low progesterone), W2 (days 7–16; high oestradiol and low progesterone) and W3 (days 17–26; intermediate oestradiol and high progesterone). RNA expression of 50 genes, including 27 ERGs, 11 putative PRGs and seven PAGs was measured. The AvERG (geomean of PGR, GREB1, TFF1 and PDZK1) was used as a composite measure of ERG expression and showed significant changes between the three windows of the menstrual cycle increasing over 2.2-fold between W1 and W2 and decreasing between W2 and W3 and between W3 and W1. Proliferation gene expression also varied significantly, following the same pattern of changes as ERG expression, but the changes were of lower magnitude (1.4-fold increase between W1 and W2). Significant changes in the expression of eight individual ERGs, including GREB1, PGR and TFF1, and two PAGs were observed between W1 and either W2 or W3 with all genes showing higher levels in W2 or W3 (1.3–2.4-fold; FDR 0.016–0.05). The AvProg, a composite measure of PRG expression, increased significantly (1.5-fold) in W3 compared to W1 or W2 but no significant changes were observed for individual PRGs. In conclusion, we observed significant changes in ERG, PRG and PAG expression in ER+ breast tumours during the menstrual cycle that may affect the assessment and interpretation of prominent biomarkers (e.g. PgR) and commonly used multigene prognostic signatures in premenopausal ER+ breast cancer.
Highly transparent conductors for optical and microwave access to spin-based quantum systems
Authors: Staacke Robert , John Roger , Kneiß Max , Grundmann Marius , Meijer Jan 
Published: November 2019

AbstractSpin-based quantum systems are among the best sensors in terms of sensitivity and spatial resolution for a variety of physical properties. A key factor to broaden the range of applications is the control over the microwave field used to manipulate the quantum system. We present the implementation of a transparent microwave conductor, suitable for optical read-out of a spin-based quantum sensor. For this, the interaction of an indium tin oxide (ITO) strip line on diamond with nitrogen vacancy centres was investigated. The amorphous ITO can be fabricated at room temperature, has a transmittance larger than 80% in the visible spectrum and a low resistivity. We show that these strip lines are completely scalable which enables highly homogeneous microwave fields from the nanometre up to the millimetre scale. ITO structures can therefore serve as excellent transparent microwave conductors, widening the current use of spin-based quantum sensors.
The effect of combined application of Streptomyces rubrogriseus HDZ-9-47 with soil biofumigation on soil microbial and nematode communities
Authors: Jin Na , Lu Xiuliang , Wang Xueyan , Liu Qian , Peng Deliang , Jian Heng 
Published: November 2019

AbstractMeloidogyne incognita causes significant damage to many different crops. Previous studies showed that Streptomyces rubrogriseus HDZ-9-47 is a promising biocontrol agent. Combining it with biofumigation improved its efficacy against M. incognita. In the present study, the reason for the improved efficacy of the combination was investigated by analyzing its impact on both the soil microbial and the nematode communities in the field. The results showed that the combined application reduced root galls by 41% and its control efficacy was greater than each treatment alone. Cultivation-based analyses showed that the combination treatment affected the soil microbial community. Actinomycetes and bacterial densities were negatively correlated with the root knot score. In contrast, the fungal densities were positively correlated with the root knot score. Denaturing gradient gel electrophoresis (DGGE) results showed that the combination of S. rubrogriseus HDZ-9-47 and biofumigation enriched beneficial microbes and reduced certain soil-borne fungal phytopathogens, thereby enhancing the efficacies of both S. rubrogriseus HDZ-9-47 and biofumigation against M. incognita. And HDZ-9-47 could colonize in soil. The total abundance of nematode and plant parasites, the ratio of soil fungivore nematode to fungivore plus bacterivore nematode, and the nematode diversity indices all decreased with the combination treatment. Overall, the results of this study demonstrate that combined application of HDZ-9-47 with biofumigation was a useful and effective approach to suppress M. incognita by manipulating soil microbial communities in field.
Somatic mutation profiling and HER2 status in KRAS-positive Chinese colorectal cancer patients
Authors: Dong Zhouhuan , Kong Linghong , Wan Zhiyi , Zhu Fengwei , Zhong Mei , Lv Yali , Zhao Po , Shi Huaiyin 
Published: November 2019

AbstractKRAS is an independent negative predictor for anti-epidermal growth factor receptor (anti-EGFR) treatment in colorectal cancers (CRCs). However, 30% to 50% of CRC patients are KRAS-positive and do not benefit from anti-EGFR therapy. In this study, we investigated the mutational features and clinical significance of KRAS-positive Chinese CRC patients. A total of 139 Chinese CRC patients who received clinical KRAS testing (Sanger sequencing) were examined by immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH). Fifty KRAS-positive specimens were further detected by next-generation sequencing (NGS). The most prevalent mutation in KRAS was G12D (46%), followed by G12V (20%), and G13D (18%). In addition to KRAS, 72 unique alterations in another 12 genes were also detected. The most common mutated genes were TP53 (62%), APC (46%), and PIK3CA (22%). The proportion of HER2 amplifications in KRAS-positive CRC patients was 4.4%, which was lower than that in KRAS -negative CRC patients (14.3%). No relationship was found between HER2 amplification and KRAS status (p = 0.052). However, the odds ratio is very low (0.279). In addition, these gene mutations were not significantly associated with age, sex, tumor size, lymph node metastasis, mismatch repair–deficient, or tumor differentiation. However, TP53 mutations were more prevalent in colon cancer with KRAS mutations than in rectal cancer (75.0% vs 28.6%, respectively, p = 0.004). The negative predictive value of the IHC analysis for predicting HER2 amplification reached to 98.39%, while the positive predictive value reached only 50%. Overall, the mutation profiling of Chinese CRC patients with KRAS mutations is different from that of Western CRC patients. Our results will help us to understand the molecular features of Chinese CRC patients.
An assessment of irrigated rice production energy efficiency and environmental footprint with in-field and off-field rice straw management practices
Authors: Nguyen-Van-Hung  , Sander Bjoern Ole , Quilty James , Balingbing Carlito , Castalone Angeli Grace , Romasanta Ryan , Alberto Ma Carmelita R. , Sandro Joseph M. , Jamieson Craig , Gummert Martin 
Published: November 2019

AbstractThe research provided scientific evidences for improved rice straw management. Rice cultivation with in-field burning of rice straw is the worst option with the lowest energy efficiency and highest air pollution emission. This article comprises a comparative assessment of energy efficiency and the environmental footprint of rice production using four different rice straw management scenarios, namely, straw retained, straw burned, partial straw removal, and complete straw removal. Paddy yield, grain quality, and energy balance were assessed for two seasons while greenhouse gas emissions (GHGE) were measured weekly starting from land preparation through to the cropping and fallow period. Despite the added energy requirements in straw collection and transport, the use of collected rice straw for mushroom production can increase the net energy obtained from rice production systems by 10–15% compared to burning straw in the field. Partial and complete removal of rice straw reduces GHGE by 30% and 40% compared to complete straw retention, respectively.