bluuebunny/crossref_metadata_2025_split huggingface dataset:
Free-roaming dogs limit habitat use of giant pandas in nature reserves
Authors: Callan Ramana , Owens Jacob R. , Bi Wenlei , Kilham Benjamin , Yan Xia , Qi Dunwu , Hou Rong , Spotila James R. , Zhang Zhihe 
Published: June 2020

AbstractGiant pandas (Ailuropoda melanoleuca) were historically hunted using dogs and are currently threatened by free-roaming dogs and their associated diseases. To better understand the spatial magnitude of this threat, we used a GIS approach to investigate edge effects of dogs on giant panda habitat. We first examined two nature reserves with contrasting free-roaming dog populations: Liziping, with many dogs (~0.44/km2), and Daxiangling, with few dogs (~0.14/km2). Spatial analysis indicated that giant pandas at Liziping (but not Daxiangling) showed a shift in habitat use away from populated areas consistent with a risk response to the foray distance of free-roaming dogs (10.9 km path-distance). Most giant panda locations (86%) from the 2014 census in Liziping were clustered around remote “dog-free zones.” Expanding this analysis across the entire giant panda range revealed that 40% of panda habitat is within the foray distance of dogs. Our assessment will inform dog control programs including monitoring, education, veterinary care, and other measures. We recommend that reserves designated for the release of translocated pandas receive priority consideration for dog control efforts. Only by understanding and managing complex interactions between humans, domestic animals, and wild animals can we sustain natural systems in a world increasingly dominated by humans.
Genome-wide characterization and expression analyses of the auxin/indole-3-acetic acid (Aux/IAA) gene family in barley (Hordeum vulgare L.)
Authors: Shi Qi , Zhang Yueya , To Vinh-Trieu , Shi Jin , Zhang Dabing , Cai Wenguo 
Published: June 2020

AbstractAux/IAA genes are early auxin-responsive genes and essential for auxin signaling transduction. There is little information about Aux/IAAs in the agriculturally important cereal, barley. Using in silico method, we identified and subsequently characterized 36 Aux/IAAs from the barley genome. Based on their genomic sequences and the phylogenic relationship with Arabidopsis and rice Aux/IAA, the 36 HvIAAs were categorized into two major groups and 14 subgroups. The indication of the presence or absence of these domains for the biological functions and acting mechanisms was discussed. The cis-element distributions in HvIAA promoters suggests that the HvIAAs expressions may not only regulated by auxin (the presence of AuxREs and TGA-element) but also by other hormones and developmental and environmental cues. We then studied the HvIAAs expression in response to NAA (1-Naphthaleneacetic acid) using quantitative real-time PCR (qRT-PCR). Like the promoter analysis, only 14 HvIAAs were upregulated by NAA over two-fold at 4 h. HvIAAs were clustered into three groups based on the spatiotemporal expression data. We confirmed by qRT-PCR that most HvIAAs, especially HvIAA3, HvIAA7, HvIAA8, HvIAA18, HvIAA24 and HvIAA34, are expressed in the developing barley spike compared within seedling, suggesting their roles in regulating spike development. Taken together, our data provide a foundation for further revealing the biological function of these HvIAAs.
Chemical vs. mechanical microstructure evolution in drying colloid and polymer coatings
Authors: Kaewpetch Thitiporn , Gilchrist James F. 
Published: June 2020

AbstractColloidal based films have been widely developed for a wide range of applications including chemical and electrical barrier coatings, photonic materials, biomaterials, and pharmaceutical oral drug delivery. Many previous studies investigate methods to generate uniformity or desired stratification of the final components with a desired microstructure. Few studies have been able to investigate this microstructure in-situ during drying. This experimental study directly tracks fluorescent colloids that are either stable in suspension or have attractive interactions during the drying process using high speed laser scanning confocal microscopy to obtain details of microstructural evolution during drying. The colloidal microstructure in stable suspensions evolves continuously during drying. Microstructures in these systems have a signature Voronoi polyhedra distribution that is defined by lognormal curve having a constant standard deviation that only depends on its chemical composition. Those formulations having strongly attractive constituents have microstructure that is heterogeneous and non-monotonic due to the mechanics associated with internal convection and capillary forces. Toward the end of drying, the influence of the mode of microstructure rearrangements remains evident.
Identification and field bioassays of the sex pheromone of Eurytoma maslovskii (Hymenoptera: Eurytomidae)
Authors: Yang Chang Yeol , Mori Kenji , Kim Junheon , Kwon Ki Bong 
Published: June 2020

AbstractLong-range sex pheromones are generally considered to be a main cue for mate recognition in the order Hymenoptera. Although considerable attention has been given to the identification of semiochemicals in the superfamily Chalcidoidea, which comprises 19 families, no study has identified active components in a field bioassay. We herein report the sensitive and selective pheromone communication system of the Korean apricot wasp, Eurytoma maslovskii (Eurytomidae), whose larvae feed on Prunus mume seeds. Using gas chromatography coupled with electroantennographic detection (GC-EAD) and mass spectrometry (GC-MS), we detected 2,10-dimethyldodecyl propionate and 2,8-dimethyldecyl propionate in thoracic extracts of female E. maslovskii at a ratio of 8:2 as the active pheromone components. Field experiments showed that the attractive effect of the two compounds is highly enantioselective. Racemic 2,10-dimethyldodecyl propionate and 2,8-dimethyldecyl propionate were not attractive to E. maslovskii males. In bioassays with single enantiomers, the (2 S,10 R)-enantiomer was highly attractive to male wasps, and the (2 S,8 S)-enantiomer was also attractive, although to a lesser degree. No synergistic effect between (2 S,10 R)- and (2 S,8 S)-enantiomers was identified, and the (2 S,10 R)-enantiomer alone caught significantly more males than the natural pheromone extracts. The addition of other enantiomers to the (2 S,10 R)-isomer significantly decreased the attraction of conspecific males. In addition, a very low dose of synthetic pheromone attracted conspecific males, showing that both female signaling and male response traits may have evolved to contribute to species-specific sexual communication in this species.
P300 response modulation reflects breaches of non-probabilistic expectations
Authors: Valakos D. , d’Avossa G. , Mylonas D. , Butler J. , Klein C. , Smyrnis N. 
Published: June 2020

AbstractIn oddball paradigms, infrequent stimuli elicit larger P300 event related potentials (ERPs) than frequent ones. One hypothesis is that P300 modulations reflect the degree of “surprise” associated with unexpected stimuli. That is the P300 represents how unlikely the stimulus is and this signal is then used to update the observer’s expectations. It could be hypothesized that P300 is modulated by any factor affecting an observer’s expectations, not only target probability. Alternatively, the P300 may reflect an evaluative process engaged whenever a discrepancy between task context and sensory inputs arises, irrespective of the latter probability. In previous ERP studies, stimulus probability was often the only determinant of task set confounding the effects of stimulus probability and set stimulus discrepancy. In this study, we used a speeded luminance detection task. The target was preceded by a central cue that predicted its location. The probability that the target was valid, i.e. would appear at the cued location was manipulated by varying the reliability of the cue. Reaction times were modulated by probabilistic expectations based on cue reliability and target validity while P300 was affected by target validity only. We conclude that increased P300 amplitude reflects primarily breaches of non-probabilistic expectations, rather than target probability.
Immune responses in beta-thalassaemia: heme oxygenase 1 reduces cytokine production and bactericidal activity of human leucocytes
Authors: Nithichanon Arnone , Tussakhon Inthira , Samer Waraporn , Kewcharoenwong Chidchamai , Ato Manabu , Bancroft Gregory J. , Lertmemongkolchai Ganjana 
Published: June 2020

AbstractPatients with beta-thalassaemia increase the risk of bacterial infections, particularly Burkholderia pseudomallei (Bp), the causative agent of melioidosis in Thailand. Impaired immune cell functions may be the cause of this susceptibility, but detailed mechanisms have not been defined. In this study, we observed impaired production of IFN-gamma and IL-10 by whole blood from beta-thalassaemia patients upon stimulation with a range of bacteria-derived stimuli. In contrast, IFN-gamma response via TCR and plasma IgG specific for Bp were still intact. Importantly, mRNA expression of heme oxygenase 1 (HO-1), a potential modulator of immune function, was increased in whole blood from beta-thalassaemia patients, either with or without stimulation with Bp in vitro. Induction of HO-1 by hemin or CoPP in vitro reduced production of IFN-gamma and IL-10 from healthy human PBMCs and decreased bacterial clearance activity of whole blood from healthy controls and beta-thalassaemia, while inhibition of HO-1 by SnPP enhanced both functions in healthy controls. These results were confirmed to some extent in purified human monocytes of healthy controls. Our results suggest a mechanism that excess hemin of beta-thalassaemia patients is a significant cause of immune suppression via HO-1 induction and may underlie the susceptibility of these individuals to severe bacterial infection.
The role of Rho GTPases’ substrates Rac and Cdc42 in osteoclastogenesis and relevant natural medicinal products study
Authors: Liu Yuan , Dou Yusheng , Yan Liang , Yang Xiaobin , He Baorong , Kong Lingbo , Smith Wanli 
Published: June 2020

Abstract
Recently, Rho GTPases substrates include Rac (Rac1 and Rac2) and Cdc42 that have been reported to exert multiple cellular functions in osteoclasts, the most prominent of which includes regulating the dynamic actin cytoskeleton rearrangements. In addition, natural products and their molecular frameworks have a long tradition as valuable starting points for medicinal chemistry and drug discovery. Although currently, there are reports about the natural product, which could play a therapeutic role in bone loss diseases (osteoporosis and osteolysis) through the regulation of Rac1/2 and Cdc42 during osteoclasts cytoskeletal structuring. There have been several excellent studies for exploring the therapeutic potentials of various natural products for their role in inhibiting cancer cells migration and function via regulating the Rac1/2 and Cdc42. Herein in this review, we try to focus on recent advancement studies for extensively understanding the role of Rho GTPases substrates Rac1, Rac2 and Cdc42 in osteoclastogenesis, as well as therapeutic potentials of natural medicinal products for their properties on the regulation of Rac1, and/or Rac2 and Cdc42, which is in order to inspire drug discovery in regulating osteoclastogenesis.
Voice Therapy for Benign Voice Disorders in the Elderly: A Randomized Controlled Trial Comparing Telepractice and Conventional Face-to-Face Therapy
Authors: Lin Feng-Chuan , Chien Hsin-Yu , Chen Sheng Hwa , Kao Yi-Chia , Cheng Po-Wen , Wang Chi-Te 
Published: June 2020

PurposePrevious studies have reported that voice therapy via telepractice is useful for patients with nodules and muscle tension dysphonia. Nevertheless, telepractice for elderly patients with voice disorders has not yet been investigated. We conducted this study to examine the hypothesis that voice therapy via telepractice is not inferior to conventional voice therapy.MethodEighty patients with dysphonia aged more than 55 years participated in this study from September 2016 to June 2018. After screening the inclusion and the exclusion criteria, 69 patients were randomized into telepractice (33 patients) and conventional (36 patients) groups. The outcome measurements included Voice Handicap Index-10, videolaryngostroboscopy, maximum phonation time, auditory-perceptual evaluation, and acoustic analysis. Pairedttest, Wilcoxon signed-ranks test, and repeated measures analysis of variance were used to examine treatment outcomes.ResultsThe diagnoses of voice disorders included atrophy (n= 33), unilateral vocal paralysis (n= 13), muscle tension dysphonia (n= 7), nodules (n= 6), and polyps (n= 10). No significant differences were observed in age, sex, and baseline measurements between the two groups. Twenty-five patients in the telepractice group and 24 patients in the control group completed at least four weekly sessions. Significant improvements were observed for all the outcome measures (p< .05) in both groups. Improvements in Voice Handicap Index-10 in the telepractice group (24.84 ± 5.49 to 16.80 ± 8.94) were comparable to those in the conventional group (22.17 ± 7.29 to 13.46 ± 9.95,p= .764). Other parameters also showed comparable improvements between the two groups without statistically significant differences.ConclusionsThis is the first randomized controlled trial comparing telepractice and conventional voice therapy in elderly patients with voice disorders. The results showed that the effectiveness of voice therapy via telepractice was not inferior to that of conventional voice therapy, indicating that telepractice can be used as an alternative to provide voice care for elderly patients with vocal disorders.
Tidal distortion and disruption of rubble-pile bodies revisited
Authors: Zhang Yun , Michel Patrick 
Published: June 2020

Context.In the course of a close approach to planets or stars, the morphological and dynamical properties of rubble-pile small bodies can be dramatically modified, and some may catastrophically break up, as in the case of comet Shoemaker-Levy 9. This phenomenon is of particular interest for the understanding of the evolution and population of small bodies, and for making predictions regarding the outcomes of future encounters. Previous numerical explorations have typically used methods that do not adequately represent the nature of rubble piles. The encounter outcomes and influence factors are still poorly constrained.Aims.Based on recent advances in modeling rubble-pile physics, we aim to provide a better understanding of the tidal encounter processes of rubble piles through soft-sphere discrete element modeling (SSDEM) and to establish a database of encounter outcomes and their dependencies on encounter conditions and rubble-pile properties.Methods.We performed thousands of numerical simulations using the SSDEM implemented in theN-body codepkdgravto study the dynamical evolution of rubble piles during close encounters with the Earth. The effects of encounter conditions, material strength, arrangement, and resolution of constituent particles are explored.Results.Three typical tidal encounter outcomes are classified, namely: deformation, mass shedding, and disruption, ranging from mild modifications to severe damages of the progenitor. The outcome is highly dependent on the encounter conditions and on the structure and strength of the involved rubble pile. The encounter speed and distance required for causing disruption events are much smaller than those predicted by previous studies, indicating a smaller creation rate of tidally disrupted small body populations. Extremely elongated fragments with axis ratios ~1:6 can be formed by moderate tidal encounters. Our analyses of the spin-shape evolution of the largest remnants reveal reshaping mechanisms of rubble piles in response to tidal forces, which is consistent with stable rubble-pile configurations derived by continuum theory. A case study for Shoemaker-Levy 9 suggests a low bulk density (0.2–0.3 g cc−1) for its progenitor.
The Great Masquerader: Tuberculosis Presenting as Community-Acquired Pneumonia
Authors: Dheda Keertan , Makambwa Edson , Esmail Aliasgar 
Published: June 2020

AbstractAccording to World Health Organization estimates, tuberculosis (TB) and lower respiratory tract infections (LRTIs) are both among the top 10 global causes of death. TB and community-acquired pneumonia (CAP), if mortality estimates are combined, would rank as the third most common cause of death globally. It is estimated that each year there are approximately 10 million new cases of TB that are associated with approximately 1.2 million deaths, and almost 450 million new episodes of LRTI (synonymous with CAP) with approximately 4 million associated deaths. Globally, Streptococcus pneumoniae remains the most common cause of CAP. However, although well documented, it is not widely appreciated that in several parts of the world, including sub-Saharan Africa, Asia, and South America, Mycobacterium tuberculosis is an important cause of CAP, if not the most common organism isolated in such settings. Thus, CAP due to M. tuberculosis is not uncommon in some parts of the world with up to a third of cases being attributable to M. tuberculosis. Consequently, TB remains an important clinical entity in the intensive care unit in these settings. Despite its frequency and importance, there are very limited data about TB CAP. In this review we discussed the epidemiology, immunopathogenesis, clinical presentation, diagnosis, management, prognosis, and prevention of TB CAP. The utility of newer diagnostic approaches is highlighted.
Laparoscopic Vascular Hitch for Polar Vessels in Pyeloureteric Junction Obstruction: Medium-Term Follow-up of a Monocentric Experience
Authors: Al-Emadi Ibrahim , Juricic Mélodie , Mouttalib Sofia , Galinier Philippe , Bouali Ourdia , Abbo Olivier 
Published: June 2020

Abstract
Introduction To evaluate medium-term results of the management of patients with pyeloureteric junction obstruction (PUJO) secondary to lower pole vessels managed by a laparoscopic vascular hitch technique.
Materials and Methods We performed a retrospective monocentric study of all patients who were operated using this technique, transperitoneal laparoscopy (TL) or robotic surgery (RS), from 2011 to 2018. Description of the initial population, pre- and postoperative data was collected and analyzed.
Results During the study period, 25 patients (11 males and 14 females) with a mean age of 11.1 years were managed. Initial symptoms leading to the diagnosis were present in 21 children (lumbar pain 17, high blood pressure 1, and infection 3). Four patients were diagnosed incidentally (three) or following a prenatal screening (one). The diagnostic has been confirmed in all patients by ultrasound scan completed by computed tomography or magnetic resonance imaging scans. Twenty-one patients were operated by TL and four by RS. Mean operating time was 90.2 minutes. Three immediate postoperative complications were noticed including one urinary tract infection, one urinary tract rupture, and one postoperative massive dilatation. After a mean follow-up of 3 1.5 years, 17 patients remain asymptomatic. Mean pelvic diameter was preoperatively 35 mm and postoperatively 10.5 mm (p < 0.005). Of the eight remaining patients, six required reoperation (pyeloplasty) and two remained symptomatic with recurrent flank pain.
Conclusion In our experience, PUJO managed by the vascular hitch technique is associated with a 68% success rate after a 3-year follow-up. These results are different from the current literature which reported success rate of 96% after 52 months. It leads us to question the efficiency of this technique in the long term in our hands both concerning the indications and the surgical procedure itself. Further studies are required to collect data concerning the evolution of our patients during adulthood to compare their evolution to classic pyeloplasty.
Health-Related Quality of Life in Patients with the Bladder Exstrophy-Epispadias Complex and Relationship to Incontinence and Sexual Factors: A Review of the Recent Literature
Authors: Holmdahl Gundela , Dellenmark-Blom Michaela , Nordenskjöld Agneta , Sjöström Sofia 
Published: June 2020

AbstractThe bladder exstrophy-epispadia complex (BEEC) includes malformations with midline closing defects of the lower abdomen and external genitalia. Long-term consequences with urinary incontinence and sexual dysfunction, in spite of multiple surgical interventions, are common and expected to affect the patient's health-related quality of life (HRQOL). The extent and the predictive factors are, however, not known. New patient-reported outcome research is emerging, but valid and reliable condition-specific HRQOL are still missing. The aim of this review is to summarize and discuss the latest published reports (2015–2019) on HRQOL in patients with the BEEC and its relationship to incontinence and sexual factors.
Invention, Innovation, and Imitation in India—Necessity Arising from the COVID-19 Pandemic
Authors: Mathew Joseph L. , Mathew T. Lazar 
Published: June 2020

AbstractThe COVID-19 pandemic has stimulated tremendous innovation in India, driven by unprecedented novel needs, sense of urgency, availability of time during the lockdown, funding by Government agencies, and accelerated processing of projects. Therefore, the country has witnessed widespread innovation and imitation of existing technologies in recent weeks, but very few inventions. One novel invention, the Artificial Breathing Capability Device (ABCD) is being speeded up by the current necessity. Other innovations and imitations include (i) healthcare devices/products intended for diagnosis, management, or monitoring of suspected/confirmed COVID-19 patients (in healthcare institutions); (ii) improvisations in personal protective equipment (PPE) for frontline healthcare workers and other personnel (including the public); (iii) adaptation of information technology platforms for telehealth, telemedicine, telemonitoring, and tele-education; and (iv) repurposing of existing resources/technologies for COVID-19. Some fruits of these efforts include (i) respiratory support devices (by mechanization of manual ventilation, ventilator splitters, etc.), (ii) personal protective equipment (PPE) (novel face masks, face shields/splash guards, intubation boxes, aerosol guards, etc.), (iii) novel sanitization products, processes or deployment methods, (iv) diagnostic tests (including rapid antibody tests, polymerase chain reaction [PCR] methods, and loop-mediated isothermal amplification [LAMP]), and (v) various adaptations such as repurposing and retrofitting of existing technologies aligned to the needs of COVID-19. These innovations and imitations have laudable features such as extremely rapid development of products (not merely concepts or prototypes); collaboration among individuals, institutions, and industry; willingness of players to work outside traditional comfort zones; and teamwork among stakeholders. Social media and lay press publicity create a sense of achievement and hope in a nation bogged down by the realities of COVID-19. However, these positive developments are associated with unique problems, especially duplication of effort, disregard of intellectual property (IP) issues, unhealthy competition amongst institutions, and failure to consider the needs for which solutions are proposed. The haste for developing products has bypassed the important steps of peer review, laboratory testing, and clinical validation to ensure the safety and efficacy of end users. These unintended side effects of the innovation race can be overcome by coordinated efforts through an innovation pathway proposed in this article under a broader innovation ecosystem.