bluuebunny/crossref_metadata_2025_split huggingface dataset:
Tongfu Huoxue Decoction Reduces Autophagy and Apoptosis to Protect Against Acute Renal Injury in a Rat Model of Sepsis
Authors: Wang Yan , Wang Zixuan , Yang Qianlan , Fan Yuxin , Wang Lu , Zhang Yunhe , Du Lijian 
Published: December 2020

Abstract
Background: To investigate the mechanism by which Tongfu Huoxue decoction protects against acute kidney injury in sepsis in rats, and provide a new therapeutic strategy for clinical intervention.Methods: Thirty-two Sprague–Dawley rats were randomly divided into a control group (saline), model group (lipopolysaccharide LPS group), LPS group+Tongfu Huoxue Decoction high-dose group (H group), and LPS group +Tongfu Huoxue Decoction low-dose group (L group). A rat model of acute kidney injury in sepsis was established by administration of LPS, and Tongfu Huoxue decoction was administered by gavage. The pathological and morphological changes of the kidneys were evaluated according to the levels of urea nitrogen (BUN) and blood creatinine (SCr). The expression of the inflammation-related factors IL-1b, IL-6 and TNF-a was determined by quantitative real-time PCR. Changes in the phosphorylation levels of the autophagy and apoptosis-related proteins LC3, beclin-1, caspase-3 and Akt were detected by Western blot analysis. Results: Compared with the model LPS group, kidney tissue damage was reduced in rats treated with Tongfu Huoxue decoction. The expression levels of inflammation-related and autophagy-related proteins in the kidney tissue of rats treated with Tongfu Huoxue decoction were significantly lower than those in the LPS group, which was showed a dose-dependent decrease in expression. After stimulation of HK-2 cells with LPS, the expression levels of the autophagy and apoptosis in the groups treated with Tongfu Huoxue decoction decreased in a dose-dependent manner. Furthermore, the rate of HK-2 cell apoptosis was higher in the LPS group than that in the control group, with lower rates in the H and L groups than that in the LPS group and were dose-dependent.Conclusion: Tongfu Huoxue decoction protects against acute renal injury in sepsis by reducing autophagy and apoptosis.
Estimation of economic burden of COVID-19 using Disability-Adjusted Life Years (DALYs) and Productivity Losses in Kerala state, India
Authors: John Denny , MS Narassima , Menon Jaideep C , Jammy Guru Rajesh 
Published: December 2020

Abstract
Background: The COVID-19 pandemic has had a huge impact on the global economy and stressed the health care systems worldwide. Measuring the burden of disease on health and economy is essential for system preparedness by way of allocation of funds and human resources.Methods: The present study estimates Disability-Adjusted Life Years (DALYs), Years of Potential Productive Life Lost (YPPLL) and Cost of Productivity Lost (CPL) due to premature mortality and absenteeism, secondary to COVID-19 in Kerala state, India. The impact of disease on various age-gender cohorts has been analyzed. Sensitivity Analysis has been conducted by adjusting six variables with a total of 21 scenarios. Results: Severity of infection and mortality were higher among older sub-group of patients, and male were more susceptible than female in most of the age groups. DALY for the baseline scenario was 15,924.24 and 8,669.32 for males and females respectively. The CPL due to premature mortality was 26,80,36,179 and 42,510,946 for males and females respectively. Conclusion: People aged more than 50 were disproportionately affected by the disease, with presence of comorbidities further raising vulnerability.
Hyaluronate-based Self-stabilized Nanoparticles for Reverting Immunosuppression and Immunochemotherapy in Osteosarcoma Treatment&nbsp;
Authors: Zhang Yi , Yuan Tao , Li Zuxi , Luo Chunyang , Wu Yuxuan , Zhang Jiyong , Zhang Xiao , Fan Weimin 
Published: December 2020

Abstract
Background: Chemotherapy and immunotherapy are the mainly non-surgical treatment for osteosarcoma in clinic presently. However, serious side effects, short blood circulation time, and immunosuppressive tumor microenvironment have extremely limited their application. To generate an onsite combined chemotherapy and immunotherapy regimen, we designed a self-stabilized hyaluronic acid (HA) nanoparticle for the tumor-targeting delivery of doxorubicin (DOX), cisplatin (DDP), and resiquimod (R848) in osteosarcoma treatment, referred to as DDPNPDOX&R848. Methods: Here, we tested the physicochemical properties of and the ability of DDPNPDOX&R848 to induce immunogenic cell death (ICD) and revert immunosuppressive cells were characterized in vitro. The therapeutic efficacy of DDPNPDOX&R848 in vivo was evaluated in an orthotopic osteosarcoma mouse model. Furthermore, we also verified the immune memory evoking effect of DDPNPDOX&R848 in an osteosarcoma rechanllenge mouse model.Results: DDPNPDOX&R848 possesses a uniform size (similar to 190 nm) and ideal pH responsibility, and its ability to induce ICD and modulate immune cells was also proven. In vivo, regardless of the mouse model used (i.e., orthotopic osteosarcoma or rechanllenge xenografted osteosarcoma), DDPNPDOX&R848 showed significantly enhanced tumor inhibition and prolonged survival. Evoked tumoricidal immune memory responses were also demonstrated in the rechanllenged osteosarcoma mouse model.Conclusion: In summary, this intelligent codelivery platform might be a competitive candidate for osteosarcoma immunochemotherapy.
Evaluation of a novel and rapid screening method for the detection of contaminated colistin-resistant bacteria in food
Authors: Vu Hanh , Appiah-Kwarteng Cornelia , Tanaka Kaori , Kawahara Ryuji , Khong Diep Thi , Nguyen Thang Nam , Tran Hoa Thi , Van Chinh Dang , Nguyen Phuc Do , Yamamoto Yoshimasa 
Published: December 2020

Abstract
Background: The dissemination of colistin-resistant bacteria carrying the colistin-resistant mobile gene, mcr-1 threatens medical care worldwide. In particular, contamination of food with colistin-resistant bacteria accelerates the community dissemination of colistin-resistant bacteria. Therefore, monitoring of colistin-resistant bacteria in food is important for controlling resistant bacteria. Unfortunately, the conventional culture methods for detecting colistin-resistant bacteria are not practical for monitoring food saftey. Therefore, development of a simple and rapid method to detect food contamination with colistin-resistant bacteria is desirable as an effective means for preventing the dissemination of resistant bacteria, particularly colistin-resistant bacteria.Findings: We developed a simple and rapid method for detecting Escherichia coli harboring the mcr-1 colistin resistance gene using a high-speed real-time polymerase chain reaction (PCR). The entire procedure, from sample processing to finals results, was performed within one hour. The practical utility of this method was verified by analyzing 27 retail meat samples for the presence of colistin-resistant bacteria. The results of the developed method were in agreement with the results of culturing colistin-resistant E. coli from the meat samples, demonstrating its efficacy and usefulness.Conclusions: A simple and rapid real-time PCR-based screening method was developed for detecting E. coli harboring mcr-1 in food samples. The practical utility of the procedure was confirmed using retail meat samples, indicating its potential as a convenient and rapid method to detect bacterial contamination of food items, especially in developing communities.
Gut microbiota-derived 5-hydroxyindole-3-acetic acid mediates the anti-colitis effect of Phellinus gilvus in mice
Authors: Li Yougui , Sun Yuqing , Zhong Shi , Huo Jinxi , Sun Le , Zhang Fang , Zhu Jianxun , Wu Chongming 
Published: December 2020

Abstract
Background: The gut microbiota plays crucial roles in various diseases and mediates the therapeutic effect of many drugs. Results: In this study, we found that ingestion of Phellinus gilvus extract (SH), a traditional Chinese medical mushroom, effectively ameliorated dextran sodium sulfate (DSS)-induced colits in mice, and the gut microbes were essential for its therapeutic effect. The enrichment of Alistipes spp. especially A. onderdonkii was highly associated with the amelioration of colitis by SH, and oral gavage of live A. onderdonkii potently and strain-specifically mitigated DSS-induced colitis in mice. 5-Hydroxyindole-3-acetic acid (5HIAA), a structural analogue of a known aromatic hydrocarbon receptor (AhR) activator indole-3-acetic acid (IAA), was significantly enriched after SH treatment and exhibited more potent anti-colitis efficiency than IAA in mice.Conclusion: Our results demonstrated the gut microbiota-derived 5HIAA could be an effective candidate in treatment of colitis, and modulation of the gut microbiota-indole derives-AhR pathway may be a novel strategy in development of next-generational anti-colitis drugs.
The hidden curve behind COVID-19 outbreak: the impact of delay in treatment initiation in cancer patients and how to mitigate the additional risk of dying – the head and neck cancer model
Authors: Matos Leandro L. , Forster Carlos Henrique Q. , Marta Gustavo N. , Junior Gilberto Castro , Ridge John A. , Hirata Daisy , Miranda-Filho Adalberto , Hosni Ali , Sanabria Alvaro , Gregoire Vincent , Patel Snehal G. , Fagan Johannes J. , D’Cruz Anil K. , Licitra Lisa , Mehanna Hisham , Hao Sheng-Po , Psyrri Amanda , Porceddu Sandro , Galloway Thomas J. , Golusinski Wojciech , Lee Nancy Y. , Shiguemori Elcio H. , Matieli José Elias , Shiguemori Ana Paula A. C. , Diamantino Letícia R. , Schiaveto Luiz Felipe , Leão Lysia , Castro Ana F. , Carvalho André Lopes , Kowalski Luiz Paulo 
Published: December 2020

Abstract
Purpose: The rapid spread of the SARS-CoV-2 pandemic around the world caused most healthcare services to turn substantial attention to treatment of these patients and also to alter the structure of healthcare systems to address an infectious disease. As a result, many cancer patients had their treatment deferred during the pandemic, increasing the time to treatment initiation, the number of untreated patients (which will alter the dynamics of healthcare delivery in the post-pandemic era) and increasing their risk of death. Hence, we analyzed the impact on global cancer mortality considering the decline in oncology care during the COVID-19 outbreak using head and neck cancer, a known time-dependent disease, as a model. Methods: An online practical tool capable of predicting the risk of cancer patients dying due to the COVID-19 outbreak and also useful for mitigation strategies after the peak of the pandemic has been developed, based on a mathematical model. The scenarios were estimated by information of 15 oncological services worldwide, given a perspective from the five continents and also some simulations were conducted at world demographic data. Results: The model demonstrates that the more that cancer care was maintained during the outbreak and also the more it is increased during the mitigation period, the shorter will be the recovery, lessening the additional risk of dying due to time to treatment initiation. Conclusions: This impact of COVID-19 pandemic on cancer patients is inevitable, but it is possible to minimize it with an effort measured by the proposed model.
Urban water security priorities – an Australian industry perspective
Authors: Allan Julie V. , Kenway Steven J. , Head Brian W. 
Published: December 2020

Abstract
Urban water security is a critical element of sustainable development, and sustainable water management requires a participatory and collaborative approach across all stakeholders. However, the literature suggests that there can be diverse and potentially conflicting views within community and expert groups. This research aimed to understand the extent of views within a group of industry professionals on objectives, themes and definitions of urban water security. Using 22 semi-structured interviews with participants from Queensland, Australia, we found that, for the group, the priorities for urban water security are water quality and human health, quantity of supply to meet efficient demand, and reliability and resilience of supply systems. We also found diverse views on the importance of sustainability, water-related hazards, environment and ecosystem health, affordability and risk to water security in the urban context. We conclude that there is agreement within the water service provider group on priority needs, and suggest there is potential for community and service providers to agree on urban water security needs. The research findings support operationalisation of security concepts, highlight potential barriers to achieving urban water security, and provide insights for further engagement with urban water stakeholders.
Drug Repurposing Using Similarity-based Target Prediction, Docking Studies and Scaffold Hopping of Lefamulin
Authors: Sharma Shikha , Sharma Shweta , Pathak Vaishali , Kaur Parwinder , Singh Rajesh Kumar 
Published: December 2020

Aim:
To investigate and validate the potential target proteins for drug repurposing of
newly FDA approved antibacterial drug.

Background:
Drug repurposing is the process of assigning indications for drugs other than the
one(s) that they were initially developed for. Discovery of entirely new indications from already
approved drugs is highly lucrative as it minimizes the pipeline of the drug development process by
reducing time and cost. In silico driven technologies have made it possible to analyze molecules for
different target proteins which are not yet explored.

Objective:
To analyze possible target proteins for drug repurposing of lefamulin and their validation.
Also, in silico prediction of novel scaffolds from lefamulin has been performed for assisting
medicinal chemists in future drug design.

Methods:
A similarity-based prediction tool was employed for predicting target protein and further
investigated using docking studies on PDB ID: 2V16. Besides, various in silico tools were employed
for the prediction of novel scaffolds from lefamulin using scaffold hopping technique followed
by evaluation with various in silico parameters viz., ADME, synthetic accessibility and
PAINS.

Results:
Based on the similarity and target prediction studies, renin is found as the most probable
target protein for lefamulin. Further, validation studies using docking of lefamulin revealed the
significant interactions of lefamulin with the binding pocket of the target protein. Also, three novel
scaffolds were predicted using the scaffold hopping technique and found to be in the limit to reduce
the chances of drug failure in the physiological system during the last stage approval process.

Conclusion:
In the future, lefamulin may assist in the development of the renin inhibitors, and also,
three possible novel scaffolds with a good pharmacokinetic profile can be developed into renin
inhibitors and for bacterial infections.
Карамзин во Франции
Authors: Демин Антон Олегович 
Published: December 2020

Review of: Nikolaï Karamzin en France. L'image de la France dans les Lettres d'un voyageur russe [Nikolai Karamzin in France. The Image of France in the Letters of a Russian Traveler], sous la direction de Rodolphe Baudin. Paris: Institut d’études slaves, 2014, 228 p., ill., ISSN 0078-9976, ISBN 978-2-7204-0523-5
KONSUMSI AIR DAN PRODUKSI KARET PADA BERBAGAI SISTEM PENGATURAN JARAK TANAM DALAM KAITANNYA DENGAN KANDUNGAN AIR TANAH
Authors: Cahyo Andi Nur , Ardika Risal , Wijaya Thomas 
Published: December 2020

Kekurangan air pada saat musim kemarau menyebabkan tanaman karet menggugurkan daunnya sebagai upaya adaptasi untuk mengurangi kebutuhan air. Tujuan penelitian adalah diperoleh sistem pengaturan jarak tanam yang paling efisien dalam hubungannya dengan ekstraksi air tanah, sehingga pada saat musim kemarau kompetisi air tanah dapat dikurangi. Perlakuan dalam penelitian ini ialah monokultur dengan jarak tanam normal (A), monokultur dengan jarak tanam ganda (B), dan tumpangsari (jarak tanam ganda + klon RRIC 100) (C). Perlakuan tersebut disusun dalam rancangan acak kelompok dengan 4 ulangan. Pengamatan dilakukan terhadap parameter kadar air tanah, produksi karet, waktu terjadinya gugur daun, luas daun spesifik, dan indeks luas daun. Pengamatan terhadap kadar air tanah menunjukkan bahwa tidak terdapat beda nyata antara kadar air tanah pada semua perlakuan karena curah hujan total yang tinggi, namun pada musim kemarau terjadi perbedaan pola ekstraksi air yaitu perlakuan C mengkonsumsi air lebih cepat karena ILD yang tinggi. Produksi karet kering perlakuan C nyata lebih rendah daripada perlakuan yang lain, sedangkan perlakuan A adalah yang tertinggi. Rendahnya produksi karet kering perlakuan C disebabkan karena luas daun per pohon perlakuan C hanya sekitar 50% dari luas daun perlakuan yang lainnya. Rendahnya luas daun per pohon untuk perlakuan C ini diduga disebabkan karena terlalu tingginya kerapatan tanam perlakuan C yang mencapai lebih dari dua kali kerapatan tanam perlakuan A dan B. Diterima : 24 Agustus 2011; Disetujui : 29 November 2011How to Cite : Cahyo, A.N., Ardika, R., & Wijaya, T. (2011). Konsumsi air dan produksi karet pada berbagai sistem pengaturan jarak tanam dalam kaitannya dengan kandungan air tanah. Jurnal Penelitian Karet, 29(2), 110-117. Retrieved from http://ejournal.puslitkaret.co.id/index.php/jpk/article/view/243
KINERJA FAKTIS COKLAT DARI MINYAK JARAK KEPYAR DALAM PRODUKSI SELANG GAS LPG
Authors: Puspitasari Santi , Muslich Muslich , Syamsu Yoharmus 
Published: December 2020

Faktis coklat merupakan hasil reaksi vulkanisasi minyak nabati (jarak kepyar) pada suhu 130-160oC. Faktis coklat utamanya berfungsi sebagai bahan bantu olah kompon dan bahan penstabil dimensi pada barang jadi karet. Bahan bantu olah dapat mempermudah pencampuran dengan cara meningkatkan laju dispersi bahan pengisi dan menurunkan energi pengkomponan. Selain itu, faktis coklat dapat mempertahankan dimensi terutama pada barang karet hasil ekstruksi. Pada penelitian akan dipelajari ujicoba faktis coklat dari minyak jarak kepyar dalam pembuatan selang gas LPG. Kompon tanpa dan dengan penambahan faktis coklat komersial digunakan sebagai pembanding. Tahapan penelitian diawali dengan penyusunan formula kompon selang gas LPG kemudian pembuatan kompon selang gas LPG dalam mesin giling terbuka. Kompon yang diperoleh diperam sebelum dibentuk menjadi selang dalam mesin ekstruder. Kompon dicuplik ±150 g untuk uji karakteristik vulkanisasi dan sisanya divulkanisasi dalam autoclave. Selang LPG hasil vulkanisasi diuji sifat fisika sesuai dengan SNI 06-7213-2006/Amd 1:2008. Hasil penelitian menunjukkan bahwa faktis coklat dari minyak jarak kepyar dapat berfungsi dengan baik sebagai bahan bantu olah tetapi belum mampu mencegah terjadinya dies swell pada selang. Faktis coklat dari minyak jarak kepyar tidak berpengaruh nyata pada hasil uji karakteristik vulknisasi kompon dan sifat fisika selang gas LPG. Hal ini memperkuat fungsi faktis coklat sebagai bahan bantu olah. Secara umum, faktis coklat dari minyak jarak kepyar memiliki kinerja yang setara dengan faktis coklat komersial. Diterima : 12 Maret 2012; Disetujui : 9 Juli 2012 How to Cite : Puspitasari, S., Muslich., & Syamsu, Y. (2012). Kinerja faktis coklat dari minyak jarak kepyar dalam produksi selang gas LPG. Jurnal Penelitian Karet, 30(1), 46-53. Retrieved from http://ejournal.puslitkaret.co.id/index.php/jpk/article/view/121
OPTIMASI PRODUKSI KLON IRR SERI 200 DENGAN MENGGUNAKAN BEBERAPA SISTEM SADAP DI PENGUJIAN PLOT PROMOSI
Authors: Woelan Sekar , Junaidi Junaidi , Pasaribu Syarifah Aini 
Published: December 2020

Suatu pengujian dilakukan untuk mengetahui potensi produksi masing-masing klon IRR Seri 200 melalui penerapan beberapa sistem sadap. Disamping untuk mengoptimalkan produksi, hasil pengujian ini juga berguna sebagai salah satu pertimbangan dalam pengelompokan klon nantinya. Pengujian dilaksanakan pada areal pengujian plot promosi di Kebun Percobaan Balai Penelitian Sungei Putih, Deli Serdang – Sumatera Utara mulai tahun 2004 sampai tahun 2011. Lokasi pengujian berada pada ketinggian sekitar 54 m di atas permukaan laut (dpl) dengan jenis tanah ultisol. Material yang digunakan dalam pengujian ini adalah 1% genotipe terbaik dari klon IRR seri 200 (PP/03/96) tahun tanaman 1995. Sejumlah 21 klon IRR seri 200 diuji dan 3 klon pembanding masing-masing BPM 24, PB 217, dan PB 260. Sistem sadap yang diuji cobakan adalah S/2 d2 dan S/2 d3.ET2.5% Ga1 18/y(2w) (Apr – Des) pada tahun 2004 – 2008 dan dilanjutkan dengan sistem sadap ganda S/2 d3+S/4U d3. ET2.5% Ba1(1.5) 18/y(2w) (Apr – Des) pada tahun 2009 – 2011. Hasil pengamatan menunjukkan bahwa terdapat sembilan klon IRR seri 200 (IRR 200, IRR 202, IRR 207, IRR 209, IRR 210, IRR 215, IRR 218, IRR 219) yang memiliki pertumbuhan lilit batang lebih jagur dari ketiga klon pembanding sehingga potensial dapat disadap pada umur sekitar 3,5 tahun dengan rata-rata lilit batang sebesar 73,6 cm dan rata-rata laju pertambahan lilit batang sebesar 2,14 cm/tahun. Hasil pengamatan terhadap produktivitas tanaman menunjukkan terdapat delapan klon yang memiliki potensi produksi tinggi pada sistem sadap S/2 d2 dan S/3 d3.ET2.5% Ga1 18/y(2w) (Apr – Des) yaitu IRR 202, IRR 205, IRR 207, IRR 208, IRR 213, IRR 214, dan IRR 220. Kedelapan klon tersebut berpotensi sebagai klon unggul baru. Pengelompokkan klon IRR seri 200 berdasarkan tipologi klon menunjukkan bahwa klon IRR 202, IRR 208, IRR 210, dan IRR 220 cenderung sebagai klon quick starter karena memiliki metabolisme tinggi dan kurang responsif terhadap pemberian stimulant sedagkan IRR 213 dan IRR 214 cenderung sebagai klon slow starter karena memiliki metabolisme rendah dan lebih responsif terhadap pemberian stimulant. Disarankan agar dilakukan penelitian lanjutan sampai satu siklus tanaman (20 tahun sadap) dan dilengkapi dengan data analisis diagnosis lateks. Diterima : 21 April 2012; Disetujui : 12 Agustus 2012 How to Cite : Woelan, S., Junaidi., & Pasaribu, S. A. (2012). Optimasi produksi klon IRR seri 200 dengan menggunakan beberapa sistem sadap di pengujian plot promosi. Jurnal Penelitian Karet, 30(2), 75-85. Retrieved from http://ejournal.puslitkaret.co.id/index.php/jpk/article/view/124
AKTIFITAS METABOLISME BEBERAPA KLON KARET PADA BERBAGAI FREKUENSI SADAP DAN STIMULASI
Authors: Herlinawati Eva , Kuswanhadi Kuswanhadi 
Published: December 2020

Stimulasi merupakan bagian penting dalam sistem eksploitasi tanaman karet untuk meningkatkan produksi. Munculnya banyak klon menuntut pengetahuan mengenai hubungan antara produksi lateks dengan kondisi fisiologis tanaman untuk mengoptimalkan penggunaan stimulan, sehingga sistem eksploitasi yang diterapkan tidak mengalami over atau under eksploitasi. Penelitian bertujuan membandingkan aktivitas metabolisme klon PB 260, RRIM 600, dan PB 217 pada berbagai frekuensi sadap maupun stimulasi. Penelitian dilaksanakan di Kebun Percobaan Balai Penelitian Sembawa mulai April 2010 sampai Maret 2012 dengan Rancangan Acak Kelompok dengan 5 perlakuan sistem sadap dan 3 ulangan, menggunakan klon PB 260, RRIM 600, dan PB 217 tahun tanam 2004. Hasil penelitian menunjukkan klon berproduksi tinggi (quick starter) dengan kandungan sukrosa rendah dan fosfat anorganik tinggi seperti PB 260, hanya membutuhkan pelukaan (wounding) untuk mengaktifkan metabolisme sel lateks, tidak membutuhkan stimulan dengan frekuensi yang tinggi untuk meningkatkan produksi. Stimulasi pada klon berproduksi tinggi hanya berfungsi untuk mengurangi adanya hambatan aliran. Sementara klon RRIM 600 dan PB 217 membutuhkan stimulan untuk mengaktifkan metabolisme sel lateks. Frekuensi stimulasi optimal untuk RRIM 600 dan PB 217 masing-masing adalah 12/y dan 24/y. Diterima : 15 Mei 2013; Disetujui : 13 September 2013How to Cite : Herlinawati, E., & Kuswanhadi. (2013). Aktifitas metabolisme beberapa klon karet pada berbagai frekuensi sadap dan stimulasi. Jurnal Penelitian Karet, 31(2), 110-116. Retrieved from http://ejournal.puslitkaret.co.id/index.php/jpk/article/view/138