bluuebunny/crossref_metadata_2025_split huggingface dataset:
Gli3 Regulates Vomeronasal Neurogenesis, Olfactory Ensheathing Cell Formation, and GnRH-1 Neuronal Migration
Authors: Taroc Ed Zandro M. , Naik Ankana S. , Lin Jennifer M. , Peterson Nicolas B. , Keefe David L. , Genis Elizabet , Fuchs Gabriele , Balasubramanian Ravikumar , Forni Paolo E. 
Published: November 2019

During mammalian development, gonadotropin-releasing-hormone-1 neurons (GnRH-1ns) migrate from the developing vomeronasal organ (VNO) into the brain asserting control of pubertal onset and fertility. Recent data suggest that correct development of the olfactory ensheathing cells (OEC) is imperative for normal GnRH-1 neuronal migration. However, the full ensemble of molecular pathways that regulate OEC development remains to be fully deciphered. Loss-of-function of the transcription factor Gli3 is known to disrupt olfactory development, however, if Gli3 plays a role in GnRH-1 neuronal development is unclear. By analyzing Gli3 extra-toe mutants (Gli3Xt/Xt), we found that Gli3 loss-of-function compromises the onset of achaete-scute family bHLH transcription factor 1 (Ascl-1)+vomeronasal progenitors and the formation of OEC in the nasal mucosa. Surprisingly, GnRH-1 neurogenesis was intact in Gli3Xt/Xtmice but they displayed significant defects in GnRH-1 neuronal migration. In contrast, Ascl-1nullmutants showed reduced neurogenesis for both vomeronasal and GnRH-1ns but less severe defects in OEC development. These observations suggest that Gli3 is critical for OEC development in the nasal mucosa and subsequent GnRH-1 neuronal migration. However, the nonoverlapping phenotypes between Ascl-1 and Gli3 mutants indicate that Ascl-1, while crucial for GnRH-1 neurogenesis, is not required for normal OEC development. Because Kallmann syndrome (KS) is characterized by abnormal GnRH-1ns migration, we examined whole-exome sequencing data from KS subjects. We identified and validated aGLI3loss-of-function variant in a KS individual. These findings provide new insights into GnRH-1 and OECs development and demonstrate that humanGLI3mutations contribute to KS etiology.SIGNIFICANCE STATEMENTThe transcription factor Gli3 is necessary for correct development of the olfactory system. However, if Gli3 plays a role in controlling GnRH-1 neuronal development has not been addressed. We found that Gli3 loss-of-function compromises the onset of Ascl-1+vomeronasal progenitors, formation of olfactory ensheathing cells in the nasal mucosa, and impairs GnRH-1 neuronal migration to the brain. By analyzing Ascl-1nullmutants we dissociated the neurogenic defects observed in Gli3 mutants from lack of olfactory ensheathing cells in the nasal mucosa, moreover, we discovered that Ascl-1 is necessary for GnRH-1 ontogeny. Analyzing human whole-exome sequencing data, we identified aGLI3loss-of-function variant in a KS individual. Our data suggest thatGLI3is a candidate gene contributing to KS etiology.
PENINGKATAN PRODUKTIVITAS IKAN LELE (Clarias sp.) SISTEM BIOFLOK DI PESANTREN MODERN DARUL MA’ARIF LEGOK, INDRAMAYU
Authors: Yumna Asy Syafa , Rukmono Djumbuh , Panjaitan Amyda Suryati , Mulyono Mugi 
Published: November 2019

Ikan lele merupakan sumber protein hewani yang bernilai ekonomis dan kebutuhannya terus meningkat setiap tahun. Keunggulan teknologi bioflok yang mampu menggenjot produktivitas ikan menjadi lebih tinggi dengan penggunaan lahan yang tidak terlalu luas. Tujuan penelitian adalah mengevaluasi teknis budidaya ikan lele sistem bioflok, implementasi intervensi untuk meningkatkan produktivitas budidaya ikan lele sistem bioflok, mengevaluasi performa kinerja budidaya ikan lele sistem bioflok setelah implementasi intervensi, menganalisis finansial budidaya ikan lele sistem bioflok. Hasil penelitian menunjukkan rata-rata performansi kinerja budidaya dan produktivitas mengalami peningkatan, sedangkan, rasio konversi pakan mengalami kenaikan. Intervensi berdasarkan prioritas usulan pemecahan masalah yakni penambahan kuantitas blower dan titik aerasi, pembuatan water level, sampling mingguan dan membeli benih bersertifikat. Implementasi yang dilakukan mampu meningkatkan dari produktivitas sebesar Rp526.687 menjadi sebesar Rp1.242.187 dengan tingkat kelangsungan hidup sebesar 71%. Berdasarkan hasil analisis finansial layak untuk dilanjutkan dengan R/C Ratio sebesar 1,06.
LA EMPRESA FAMILIAR COMO CLAN: RECURSOS Y UNICIDAD
Authors: Arista Zabala Ricardo 
Published: November 2019

RESUMEN
Este trabajo se enfoca en la empresa familiar, siendo su principal objetivo realizar una revisión del constructo de la familiaridad y específicamente de la dimensión de la identidad familiar, al teorizar como un primer cercamiento, la manera en que esta se construye a partir de la integración de distintos enfoques teóricos como lo son la teoría basada en recursos, la teoría de la identidad organizacional y la estructuración organizacional rescatada de la teoría de costos de transacción. La discusión gira en torno a los orígenes de una identidad colectiva en las organizaciones y como en este caso, será un punto de diferenciación entre las empresas familiares y aquellas que no lo son mediante proposiciones lógicas construidas a partir de la revisión de la literatura relevante al tema.
İlkokul Öğrencilerinin Teknoloji Destekli Matematik Öğrenmeye Yönelik Tutum ve Kaygı Düzeylerinin İncelenmesi
Authors: HACIÖMEROĞLU Guney 
Published: November 2019

Bu araştırmada öncelikli olarak ilkokul 4. sınıf öğrencilerinin teknoloji destekli matematik öğrenmeye yönelik tutumlarını belirlemek amacıyla Pierce, Stacey ve Barkatsas (2007) geliştirilen bir ölçme aracının Türkçeye uyarlama çalışması yapılmıştır. Elde edilen sonuçlar uyarlanan ölçeğin davranışsal katılım, matematiksel güven, duyuşsal katılım ve teknoloji destekli matematik öğrenmeye yönelik tutum olmak üzere 4 alt boyuttan oluştuğunu ortaya koymuştur. Uyarlanan ölçeğin alt boyutları için Cronbach alfa güvenirlik katsayısı sırasıyla .77, .85, .82, .80 olarak hesaplanmıştır. Ölçeğin bütünü için güvenirlik katsayısı .89'dur. Uyarlanan ölçeğin geçerli ve güvenilir bir ölçme aracı olduğu belirlenmiştir. Çalışmanın ikinci aşamasında öğrencilerin matematik dersine yönelik genel olarak tutumlarının olumlu ve kaygı düzeylerinin düşük olduğu belirlenmiştir. Öğrencilerin matematik dersine yönelik tutum ve kaygı düzeyleri arasında anlamlı ve negatif bir ilişki olduğu görülmektedir. Bu derse yönelik olumlu tutumlar geliştikçe kaygı düzeyinin azaldığı söylenebilir.
Modern Austrian Novel: Endless Wanderings in the Labyrinths of Kafka's Castle
Authors: Shastina Elena , Kazakova Julia , Shastina Marina , Trofimova Lyudmila , Borisov Anatolii 
Published: November 2019

The purpose of the article is to discuss the perception of Kafka's The Castle (Das Schloss, 1922) in the novels The Peaches Killers (Die Pfirsichtöter, 1972) by Alfred Kolleritsch, Among the Bieresch (Bei den Bieresch, 1979) by Klaus Hoffer and Into the Castle (Ins Schloss, 2004) by Marianne Gruber. The reference to the writers and their works is no coincidence; preference is given to the artists whose creative manner reflects the most fashionable trends in Western European literary process - from avant-garde to postmodernism. The authors of the article deliberately arrange the analysed works in chronological order to follow the stages in the development of German postmodernism which originates from modernist literature. The universal Kafkaesque discourse suggests the existence of direct and inverse connections between the author and the reader, the extra-textual tradition and reality. The article focuses on the narrative strategies of Austrian avant-garde (Kolleritsch), analyses postmodern discourse (Hoffer, Gruber) in the Austrian literature of the second half of the 20th and early 21st centuries, reveals signs of typological similarity between the novels by Kafka, Kolleritsch, Hoffer, and Gruber, which seems productive for understanding the influence of modernist literature on the development of the postmodern paradigm in the German-language literary space. Austrian literature, to a greater extent, is fraught with the search for new forms of self-expression rather than with the artistic “overcoming the past” - the awareness of collective guilt. It brought to the forefront the authors in whose works the age of change was reflected. Literary avant-garde has been replaced by authors who skillfully “play” with the previous culture and establish a dialogue with the present. The comparative methodology is to reveal the perception of "Kafkaesque discourse" in modern Austrian literature and to draw conclusions about the ways authors treat ontological questions.
BUDAYA PEDULI DAN BUDAYA MUTU MELAYANI DI PERPUSTAKAAN SMP NEGERI 13 SURAKARTA MENUJU SEKOLAH MENYENANGKAN
Authors: Widyawati Ria 
Published: November 2019

<p>Students of SMP Negeri 13 Surakarta mostly students from families with lower<br />economic level. Most students of SMP Negeri 13 Surakarta not yet have adequate facilities for the needs of their schools at home, for example a computer or netbook, books that support learning in schools, and other school supplies. Existing facilities at the school became one of the alternatives that can help students to improve students’ quality of human resources. Library SMP Negeri 13 Surakarta has the facilities and infrastructure that can help the students to follow the teaching and learning process in schools. Concern antarsiswa and concerns of the student teachers are needed in the environment of SMP Negeri 13 Surakarta because a lot of students that should be<br />redirected in order to achieve future aspired. Library SMP Negeri 13 Surakarta also constantly improve the quality of services that the students and teachers as library members feel comfortable using the library of SMP Negeri 13 Surakarta. Quality culture serves in the library of SMP Negeri 13 Surakarta realized by improving the quality of services to library patrons, for example the use of an application library to replace the use of the manual on the process of borrowing and returning books as well as data members of the library, so as to realize the school a fun and students can enjoy the learning process, feel comfortable in the environment of SMP Negeri 13 Surakarta,especially if students are in the library.</p><p>Keywords: libraries, care, quality</p>
SMRT Sequencing of a Full-length Transcriptome Reveals Transcript Variants Involved in C18 Unsaturated Fatty Acid Biosynthesis and Metabolism Pathways at Chilling Temperature in Pennisetum giganteum
Authors: Li Qingyuan , Xiang Conglin , Xu Lin , Cui Jinghua , Fu Shao , Chen Baolin , Yang Shoukun , Wang Pan , Xie Yanfeng , Wei Ming , Wang Zhanchang 
Published: November 2019

Abstract
Background Pennisetum giganteum, an abundant, fast-growing perennial C4 grass that belongs to the genus Pennisetum, family Poaceae, has been developed as a source of biomass for mushroom cultivation and production, as a source of forage for cattle and sheep, and as a tool to remedy soil erosion. However, having a chilling-sensitive nature, P. giganteum seedlings need to be protected while overwintering in most temperate climate regions. Results To elucidate the cold stress responses of P. giganteum, we carried out comprehensive full-length transcriptomes from leaf and root tissues under room temperature (RT) and chilling temperature (CT) using PacBio Iso-Seq long reads. We identified 196,124 and 140,766 full-length consensus transcripts in the RT and CT samples, respectively. We then systematically performed functional annotation, transcription factor identification, long non-coding RNAs (lncRNAs) prediction, and simple sequence repeat (SSR) analysis of those full-length transcriptomes. Isoform analysis revealed that alternative splicing events may be induced by cold stress in P. giganteum, and transcript variants may be involved in C18 unsaturated fatty acid biosynthesis and metabolism pathways at chilling temperature in P. giganteum. Furthermore, the fatty acid composition determination and gene expression level analysis supported that C18 unsaturated fatty acid biosynthesis and metabolism pathways may play roles during cold stress in P. giganteum. Conclusions We provide the first comprehensive full-length transcriptomic resource for the abundant and fast-growing perennial grass Pennisetum giganteum. Our results provide a useful transcriptomic resource for exploring the biological pathways involved in the cold stress responses of P. giganteum.
Flow-controlled ventilation (FCV) improves regional ventilation in obese patients – a randomized controlled crossover trial
Authors: Weber Jonas , Straka Leonie , Borgmann Silke , Schmidt Johannes , Wirth Steffen , Schumann Stefan 
Published: November 2019

Abstract
Background
In obese patients, high closing capacity and low functional residual capacity increase the risk for expiratory alveolar collapse. Constant expiratory flow, as provided by the new flow-controlled ventilation (FCV) mode, was shown to improve lung recruitment. We hypothesized that lung aeration and respiratory mechanics improve in obese patients during FCV.
Methods
We compared FCV and volume-controlled (VCV) ventilation in 23 obese patients in a randomized cross-over setting. Starting with baseline (BL) measurements, ventilation settings were kept identical except for the ventilation mode related differences (VCV: I:E ratio 1:2 with passive expiration, FCV I:E ratio 1:1 with active, linearized expiration). Primary endpoint of the study was the change of end-expiratory lung volume (EELV) compared to BL ventilation. Secondary endpoints were the change of mean lung volume (MLV), respiratory mechanics and hemodynamic variables.
Results
The loss of EELV and MLV compared to BL was lower during FCV compared to VCV (ΔEELV: FCV, -126 ±207 ml; VCV, -316 ±254 ml; p < 0.001, ΔMLV: FCV, -108.2 ±198.6 ml; VCV, -315.8 ±252.1 ml; p < 0.001) and at comparable plateau pressure (PPlat: BL, 19.6 ±3.7; VCV, 20.2 ±3.4; FCV, 20.2 ±3.8 cmH2O; p = 0.441), mean tracheal pressure was higher (Pmean: BL, 13.1 ±1.1; VCV, 12.9 ±1.2; FCV, 14.8 ±2.2 cmH2O; p < 0.001). All other respiratory and hemodynamic variables were comparable between the ventilation modes.
Conclusions
This study clearly demonstrates that, compared to VCV, FCV improves regional ventilation distribution of the lung at comparable PEEP, tidal volume, PPlat and ventilation frequency.
Perceptions and experiences with District Health Information System software to collect and utilize health data in Bangladesh: A qualitative exploratory study
Authors: Begum Tahmina , Khan Shaan Muberra , Adamou Bridgit , Ferdous Jannatul , Parvez Muhammad Masud , Islam Mohammad Saiful , Kumkum Feroza Akhter , Rahman Aminur , Anwar Iqbal 
Published: November 2019

Abstract
Background:
Accurate and high-quality data are important for improving program effectiveness and informing policy.
Bangladesh’s health management information system adopted the District Health Information Software, Version 2 (DHIS2) in 2009 to capture real-time health service utilization data. However, routinely collected data are being underused because of poor data quality. We aimed to understand the facilitators and barriers of implementing DHIS2 as a way to retrieve meaningful and accurate data for reproductive, maternal and child health (RMCAH) services.
Methods:
This qualitative study was conducted in two districts of Bangladesh from September 2017 to 2018. Data collection included key informant interviews (n=11), in-depth interviews (n=23), and focus group discussions (n=2). The study participants were individuals involved with DHIS2 implementation from the community level to the national level. The data were analyzed thematically.
Results:
DHIS2 could improve the timeliness and completeness of data reporting over time. The reported facilitating factors were strong government commitment, extensive donor support, and positive attitudes toward the technology among staffs. Quality checks and feedback loops at multiple levels of data gathering points were helpful to minimize data errors. Introducing a dashboard makes DHIS2 compatible to use as monitoring tool. However, the barriers to effective DHIS2 implementation were lack of human resources, slow Internet connectivity, frequent changes to of DHIS2 versions, and maintaining both manual and electronic system side-by-side. Data in DHIS2 remains incomplete because it does not capture data from private health facilities. Having two parallel management information systems reporting the same RMNCAH indicators threatens data quality and increases the reporting workload.
Conclusion:
The overall insights from this study are expected to contribute to the development of effective strategies for successful DHIS2 implementation and building responsive health management information system. Focused strategic direction is needed to sustain the achievements of digital data culture. Periodic refresher trainings, incentives for increased performance, and an automated single reporting system for multiple stakeholders could make the system more user-friendly. A national electronic health strategy and implementation framework can facilitate creating a culture of DHIS2 use for planning, setting priorities, and decision making among stakeholder groups.
TIA and minor stroke: A qualitative study of long-term impact and experiences of follow-up care
Authors: Turner Grace M , McMullan Christel , Atkins Lou , Foy Robbie , Mant Jonathan , Calvert Melanie 
Published: November 2019

Abstract
Background Transient ischaemic attack (TIA) and minor stroke are often considered transient events; however, many patients experience residual problems and reduced quality of life. Current follow-up healthcare focuses on stroke prevention and care for other long-term problems is not routinely provided. We aimed to explore patient and healthcare provider (HCP) experiences of residual problems post-TIA/minor stroke, the impact of TIA/minor stroke on patients’ lives, and current follow-up care and sources of support. Methods This qualitative study recruited participants from three TIA clinics, seven general practices and one community care trust in the West Midlands, England. Semi-structured interviews were conducted with 12 TIA/minor stroke patients and 24 HCPs from primary, secondary and community care with framework analysis. Results A diverse range of residual problems were reported post-TIA/minor stroke, including psychological, cognitive and physical impairments. Consultants and general practitioners generally lacked awareness of these long-term problems; however, there was better recognition among nurses and allied HCPs. Residual problems significantly affected patients’ lives, including return to work, social activities, and relationships with family and friends. Follow-up care was variable and medically focused. While HCPs prioritised medical investigations and stroke prevention medication, patients emphasised the importance of understanding their diagnosis, individualised support regarding stroke risk, and addressing residual problems. Conclusion HCPs could better communicate lay information about TIA/minor stroke diagnosis and secondary stroke prevention, and improve their identification of and response to important residual impairments affecting patients.
Retrospective cohort study of trifluridine/tipiracil (TAS-102) plus bevacizumab versus trifluridine/tipiracil monotherapy for metastatic colorectal cancer
Authors: Author) Daisuke Kotani(Former Corresponding , Author) Yasutoshi Kuboki(New Corresponding , Horasawa Satoshi , Kaneko Asumi , Nakamura Yoshiaki , Kawazoe Akihito , Bando Hideaki , Taniguchi Hiroya , Shitara Kohei , Kojima Takashi , Tsuji Akihito , Yoshino Takayuki 
Published: November 2019

Abstract
Background: A previous phase I/II C-TASK FORCE study of trifluridine/tipiracil plus bevacizumab for patients with heavily pretreated metastatic colorectal cancer (mCRC) showed promising activity with an acceptable toxicity profile. This retrospective study aimed to investigate the safety and efficacy of trifluridine/tipiracil plus bevacizumab compared with trifluridine/tipiracil monotherapy in patients with heavily pretreated mCRC in clinical settings. Methods: Records of patients with mCRC refractory to standard therapies who initiated trifluridine/tipiracil plus bevacizumab from January 2016 to March 2018 or trifluridine/tipiracil monotherapy from June 2014 to December 2015 were retrospectively reviewed at our institution. Results: Totally, 60 patients received trifluridine/tipiracil plus bevacizumab and 66 received trifluridine/tipiracil monotherapy. All patients had previously received standard chemotherapy. Median progression-free survival (PFS) was 3.7 months [95% confidence interval (CI), 2.3−5.1] in the trifluridine/tipiracil plus bevacizumab group and 2.2 months (95% CI, 1.8−2.6) in the trifluridine/tipiracil monotherapy group [hazards ratio (HR) 0.69; 95% CI 0.48−0.99]. PFS rate at 16 weeks was 46.6% for the trifluridine/tipiracil plus bevacizumab group and 33.9% for the trifluridine/tipiracil monotherapy group. Although a relatively higher incidence of grade ≥3 neutropenia was observed in the trifluridine/tipiracil plus bevacizumab group than that in the other group (50.0% vs. 40.9%, p = 0.371), the incidence of febrile neutropenia was not high (3.3% vs. 7.8%, p = 0.444). Conclusions: In real-world settings, trifluridine/tipiracil plus bevacizumab prolonged PFS and helped achieve higher 16-week PFS rate compared with trifluridine/tipiracil monotherapy in patients with heavily pretreated mCRC with manageable toxicities. Trial registration: Retrospectively registered