bluuebunny/crossref_metadata_2025_split huggingface dataset:
Exploring destination brand disengagement in a top-down policy context
Authors: Bassols Narcís , Leicht Thomas 
Published: April 2020

Purpose
This paper aims to analyze the case of Cartagena, Colombia, as a case of a failed destination branding. It also broadens the findings by connecting them to the extant literature about place branding, thus making this paper more explanatory. It tries to fit the fieldwork’s findings into the two main streams of branding research (bottom-up vs top-down). This paper also gives practical insights into the destination’s network of stakeholders and discusses ways to improve the destination’s management and branding.

Design/methodology/approach
This paper uses a mixed methodology approach. Field work consisted of online questionnaire to hospitality employees in the city plus semi-structured interviews conducted with 18 “expert” stakeholders in the destination. This paper is of empirical nature.

Findings
The main cause of the destination’s brand failure is found to be the top-down approach to the place brand strategy. The literature shows that cases such as this one are more common than assumed, and a possible way out of the problem is the application of bottom-up or “mixed” approaches, as these may circumvent the problems found.

Research limitations/implications
Cases like this one illustrate very well a local context but might be difficult to transfer to other contexts, so the generalization power of this paper is limited to similar places in the sociopolitical sense of the term.

Practical implications
For place branding practitioners and destination management organizations , this paper is a call for participative approaches which include all of the stakeholders of a place.

Originality/value
This paper offers an in-depth study of a branding case in Latin America, a part of the world relatively unexplored in the branding literature. On the basis of the presented case, this paper pitches top-down versus bottom-up approaches. Finally, it explains the findings by connecting the place to its broad geographical context.
Delight spirals: the cause and consequence of employee perceived customer delight
Authors: Guidice Rebecca M. , Barnes Donald C. , Kinard Brian R. 
Published: April 2020

PurposeWith increasing competition in the marketplace, there is a greater push for exceeding customer expectations and delivering customer delight to ensure firm’s success. The main reason for this push is the beneficial outcomes for the firm. More recently, hidden benefits have been identified (i.e. elevated customer emotions can positively impact other customers and employees in the service environment). Adding to this developing literature, the current research develops a model that links antecedents and outcomes to employee perceptions of customer delight.Design/methodology/approachBoth field and panel data, as well as multiple statistical methods, were utilized to test the hypothesized relationships. The field data were collected from employees of a national specialty retailer.FindingsService climate and interpersonal influence have a positive impact on customer delight and employee perceptions of customer delight. In turn, employee perceptions of customer delight positively impact harmonious passion and job dedication. In addition, accountability for pleasing customers is a significant moderator of the relationship between employee perceptions of customer delight and harmonious passion, but not between employee perceptions of customer delight and job dedication.Research limitations/implicationsThis research contributes to the ongoing debate on the viability of customer delight as a service standard by investigating the under-studied perspective of the frontline employee.Practical implicationsThis research contributes to the debate on the value of customer delight as a service standard by investigating the under-studied perspective of the frontline employee. A key takeaway for practitioners is how to create and manage the delight spirals that can occur when customers are delighted.Originality/valueThis is the first study that evaluates antecedents and outcomes of employee-perceived customer delight in a single model. This is also the first study to measure the impact of employee perceptions of customer delight with field data.
Sharing achievement and social entrepreneurial intention
Authors: Wu Wenqing , Wang Hongxin , Wei Chun-Wang , Zheng Chundong 
Published: April 2020

PurposeThis study explores the influence of sharing achievement (i.e. sustainability, enjoyment, and economic benefits) obtained by participating in the sharing economy on social entrepreneurial intention (SEI), as well as the role of perceived social worth and social entrepreneurial self-efficacy (SESE).Design/methodology/approachThis study used multiple regression analysis on a sample of 331 MBA students aged between 24 and 48 years.FindingsThe findings indicate that sharing achievement is positively related with SEI, and perceived social worth mediates the relationship between them. Moreover, SESE not only has a moderating effect on the relationship between perceived social worth and SEI but also positively moderates the overall mediation model.Practical implicationsEntrepreneurship educators could focus on improving students' SESE through courses and training. Whereas, policymakers and decision makers should actively promote the sharing economy model and regulate its management. Moreover, existing organizers of the sharing economy should improve participants' sharing achievement.Originality/valueThis study determines the relationship between sharing achievement and SEI and enriches the push/pull theory from the perspective of pull factors in the context of the sharing economy. Moreover, by exploring the mediating effect of perceived social worth and the moderating effect of SESE, the study provides understanding on the influence mechanisms of sharing achievements on SEI.
Improving public sector knowledge sharing through communities of practice
Authors: Jørgensen Rasmus , Edwards Kasper , Scarso Enrico , Ipsen Christine 
Published: April 2020

Purpose
This paper aims to study the impact of intentionally developed communities of practice (CoPs) on knowledge sharing and practice improvement in an administrative public sector organisation (PSO).

Design/methodology/approach
A case study approach was used to analyse the impact of the CoPs intentionally developed by four different teams at a Danish PSO. The study applied a CoP development framework suggested by the literature to develop the CoPs.

Findings
Three out of the four CoPs were successfully developed, and they positively affected knowledge sharing and practice improvement. CoP participants engaged in conversations to explore individual ways of working, share knowledge and ultimately improve practice. Standardisation and boundary spanning were identified as contextual factors influencing the CoP activities.

Research limitations/implications
The findings verify the framework and contribute to a better understanding of the factors affecting the development of CoPs that positively impact knowledge sharing and practice improvements in a PSO context.

Practical implications
The study provides operations managers in PSOs with a framework for developing CoPs to improve work performance through better knowledge sharing among employees.

Originality/value
The paper provides case study evidence for the relevance of CoPs in PSO settings and highlights the necessity of investing resources in employee knowledge-sharing interactions.
Germline ancestry influences the evolutionary disease course in lung adenocarcinomas
Authors: Schenk Alina , López Saioa , Kschischo Maik , McGranahan Nicholas 
Published: April 2020

AbstractPrecision medicine relies on targeting specific somatic alterations present in a patient's tumor. However, the extent to which germline ancestry may influence the somatic burden of disease has received little attention. We estimated the genetic ancestry of non‐small‐cell lung cancer (NSCLC) patients and performed an in‐depth analysis of the influence of genetic ancestry on the evolutionary disease course. Compared with European Americans (EA), African Americans (AA) with lung adenocarcinoma (LUAD) were found to be significantly younger and smoke significantly less. However, LUADs from AAs exhibited a significantly higher somatic mutation burden, with a more pronounced tobacco carcinogen footprint and increased frequencies of alterations affecting cancer genes. Conversely, no significant differences were observed between lung squamous cell carcinomas (LUSC) from EAs and AAs. Our results suggest germline ancestry influences the somatic evolution of LUAD but not LUSC.
Performance, milk fatty acid composition and behaviour of high‐yielding Holstein dairy cows given a limited grazing period
Authors: Atkins Norton E. , Cianchi Claire , Rutter S. M. , Williams Sarah J. , Gauld Carrie , Charlton Gemma L. , Sinclair Liam A. 
Published: April 2020

AbstractThe effects of a limited grazing period on the performance, behaviour and milk composition of high‐yielding dairy cows were examined. A total of 56 Holstein cows yielding 44.7 ± 0.42 kg/day were allocated to one of four treatments in one of two, 4‐week periods. Treatments were as follows: control (C)—cows housed and offered TMR ad libitum; early grazing (EG)—cows grazed for 6 hr after morning milking then housed; delayed grazing (DG)—cows returned to housing for 1 hr after morning milking followed by grazing for 6 hr, then housed; restricted TMR (RT)—cows grazed for 6 hr after morning milking, then housed and fed TMR at 75% of ad libitum. Intake of TMR was highest in cows receiving C, intermediate in EG and DG, and lowest in RT at 26.9, 23.6, 24.7 and 20.3 kg DM/day respectively. Pasture intake was similar in cows receiving EG or DG, but was higher in RT at 2.4, 2.0 and 3.5 kg DM/day respectively. Milk yield was similar between cows receiving C, EG or DG, but lowest in RT at 45.7, 44.2, 44.9 and 41.7 kg/cow, respectively, while milk fat content of C18:3 n‐3 was increased by grazing. Cows in C spent more than 55 min/day longer lying and had three additional lying bouts/day, while lying bouts were shorter than for cows receiving EG, RT or DG. It is concluded that high‐yielding cows can be grazed for 6 hr/day with little impact on performance, provided TMR is available ad libitum when housed.
Direct autotransfusion following emergency pericardiocentesis in patients undergoing cardiac electrophysiology procedures
Authors: Barbhaiya Chirag R. , Guandalini Gustavo S. , Jankelson Lior , Park David , Bernstein Scott , Holmes Douglas , Aizer Anthony , Chinitz Larry 
Published: April 2020

AbstractIntroductionAcute hemopericardium during cardiac electrophysiology (EP) procedures may result in significant blood loss and is the most common cause of procedure‐related death. Matched allogeneic blood is often not immediately available. The feasibility and safety of direct autotransfusion in cardiac electrophysiology patients requiring emergency pericardiocentesis is unknown.MethodsWe retrospectively analyzed records of patients undergoing EP procedures at a single, tertiary care medical center who had procedure‐related acute hemopericardium requiring emergency pericardiocentesis during a 3‐year period. Procedure details, transfusion volumes, and clinical outcomes of patients who received direct autotransfusion of aspirated pericardial blood via a femoral venous sheath were compared to those of patients who did not receive direct autotransfusion.ResultsDuring the study period, 10 patients received direct autotransfusion (group 1) and outcomes were compared with those of 14 control patients who did not receive direct autotransfusion (group 2). The volume of aspirated pericardial blood was similar in groups 1 and 2 (1.6 ± 0.7 L vs 1.3 ± 1.0 L, respectively; P = .52). Amongst patients with aspirated volumes <1 L, group 1 patients (n = 4) were less likely than group 2 patients (n = 8) to require allotransfusion (0% vs 75%, P = .02). Amongst patients with aspirated volume ≥1 L, group 1 patients (n = 6) required fewer units of red cell allotransfusion than group 2 patients (n = 6) (1.5 ± 0.8 units vs 4.3 ± 2.0 units, P = .01). No procedural complications related to direct autotransfusion occurred.ConclusionsDirect autotransfusion following emergency pericardiocentesis during electrophysiology procedures requiring systemic anticoagulation is feasible and safe. The utilization of direct autotransfusion may eliminate or reduce the need for allotransfusion.
Hsa_circ_0079662 induces the resistance mechanism of the chemotherapy drug oxaliplatin through the TNF‐α pathway in human colon cancer
Authors: Lai Mingfen , Liu Guiju , Li Ruijun , Bai Hua , Zhao Jizhi , Xiao Peng , Mei Jiazhuan 
Published: April 2020

AbstractThe aim of the study was to research the biological functions of circRNA (hsa_circ_0079662) and its underlying mechanism in colorectal cancer. Drug‐resistant cell lines (HT29‐LOHP, HCT116‐LOHP, HCT8‐LOHP) were separately dealt with oxaliplatin concentration gradient (0.1‐10 μmol/L). Real‐time PCR, Western blotting, dual‐luciferase assay, miRNA pull‐down assay, coimmunoprecipitation and ELASA were performed to explore the mechanism of chemotherapy drug oxaliplatin resistance in CRC. The results showed that the expression of hsa_circ_0079662 was increased in drug‐resistant cell lines by RT‐PCR. The expression of HOXA9, TRIP6, Vcam‐1, VEGFC, MMP3, MMP9 and MMP14 was higher by Western blotting. Interaction between HOXA9 and TRIP6 in CO‐IP detection. Additionally, the cytokines TNF‐α, IL‐1 and IL‐6 were also found. In conclusion, hsa_circ_0079662, as a ceRNA binding with hsa‐mir‐324‐5p, can regulate target gene HOXA9 and induced the mechanism of chemotherapy drug oxaliplatin resistance in CRC through the TNF‐α pathway in human colon cancer.
“Please mind the gap”: A secondary analysis of discomfort and comfort in intensive care
Authors: Berntzen Helene , Bjørk Ida Torunn , Storsveen Ann‐Marie , Wøien Hilde 
Published: April 2020

AbstractAims and objectivesTo explore in depth discomfort in intensive care as experienced by patients and attended to by critical care nurses.BackgroundDiscomfort in illness is complex and persistent, and its alleviation is a challenge for nurses working in intensive care units (ICU). In previous studies, we showed that ICU patients described little actual pain but suffer from much discomfort. Critical care nurses had a systematic approach to the treatment of pain, but were more haphazard in dealing with other types of discomfort.DesignSecondary qualitative analysis of data from two previous exploratory studies.MethodsContent analysis was used on existing data from 28 interviews with ICU patients, and 16 field notes and interviews with critical care nurses. Kolcaba's Comfort Theory was applied for further analysis. The COREQ checklist was used.ResultsThree themes, “Being deprived of a functioning body”, “Being deprived of a functioning mind” and “Being deprived of integrity” characterised the discomfort experienced by ICU patients. The nurses appeared to attend to all areas of discomfort expressed by patients. In need of, and providing acknowledgment and alleviation became a common overarching theme. We identified a comfort gap caused by the discrepancy between the patients’ needs and the nurses’ achievements in fulfilling these needs.ConclusionsA gap exists between ICU patients’ comfort needs and nurses’ achievements in fulfilling these, indicating that discomfort currently is an inevitable part of the critical illness trajectory. Increased knowledge about how the brain is affected in ICU patients and more systematic approaches to assessing comfort needs and enhancing comfort may support nurses in fulfilling patient needs and possibly diminish the existing comfort gap.Relevance for clinical practiceAn increased understanding of the complex experience of discomfort in ICU patients may bring about more systematic approaches to enhance comfort and direct for education and further research.
Sleep among primary care physicians–Association with overtime, night duties and strategies to counteract poor sleep quality
Authors: Gustavsson Katarzyna , Wierzbicka Aleksandra , Matuszczyk Michał , Matuszczyk Maciej , Wichniak Adam 
Published: April 2020

AbstractPhysicians belong to the risk group for sleep disorders as a result of work related stress, excessive working time, large amount of on‐call duties and shift work. Poor sleep quality of healthcare providers threatens not only their safety, but also the safety of their patients. This study examines if there are any differences in how working either long hours or at night duties relates to self‐reported sleep time, sleep quality and daytime sleepiness in primary care physicians. We analyzed data from a survey research of 807 participants. Respondents were divided into four groups based on their reported work hours per week and number of nights on duty per month. Overtime was associated with shorter sleep time and worse subjective sleep quality even when compared with that of participants who work on night duties. All investigated groups of participants reported short sleep latencies which suggests that sleep debt is a common problem in this population. Surprisingly in the case of poor sleep quality participants rarely used recommended methods like regular physical activity or specialist advice. The most frequently reported answer was that they do nothing to improve sleep and every third participants uses hypnotics to do that. These results suggest that primary care physicians despite being at high risk for sleep disorders due to working overtime and at night, pay less attention to their sleep quality and do not use recommended strategies to improve it.
Cell‐type‐resolved proteomic analysis of the human liver
Authors: Ölander Magnus , Wiśniewski Jacek R. , Artursson Per 
Published: April 2020

AbstractBackground & AimsThe human liver functions through a complex interplay between parenchymal and non‐parenchymal cells. Mass spectrometry‐based proteomic analysis of intact tissue has provided an in‐depth view of the human liver proteome. However, the predominance of parenchymal cells (hepatocytes) means that the total tissue proteome mainly reflects hepatocyte expression. Here we therefore set out to analyse the proteomes of the major parenchymal and non‐parenchymal cell types in the human liver.MethodsWe applied quantitative label‐free proteomic analysis on the major cell types of the human liver: hepatocytes, liver endothelial cells, Kupffer cells and hepatic stellate cells.ResultsWe identified 9791 proteins, revealing distinct protein expression profiles across cell types, whose in vivo relevance was shown by the presence of cell‐type‐specific proteins. Analysis of proteins related to the immune system indicated that mechanisms of immune‐mediated liver injury include the involvement of several cell types. Furthermore, in‐depth investigation of proteins related to the absorption, distribution, metabolism, excretion and toxicity (ADMET) of xenobiotics showed that ADMET‐related tasks are not exclusively confined to hepatocytes, and that non‐parenchymal cells may contribute to drug transport and metabolism.ConclusionsOverall, the data we provide constitute a unique resource for exploring the proteomes of the major types of human liver cells, which will facilitate an improved understanding of the human liver in health and disease.
Noun phrase complexity in Ghanaian English
Authors: Brato Thorsten 
Published: April 2020

AbstractThis study compares the complexity of the noun phrase (NP) in Ghanaian English in a real‐time perspective. Based on the Historical Corpus English in Ghana (1966–1975) and the Ghanaian component of the International Corpus of English (mainly 2000s), representing the early and late stages of structural nativisation in the dynamic model, NP complexity is measured using five criteria (NP pattern, complexity of the premodifier, length of the pre‐ and postmodifier and complexity of the postmodifier). The study uses conditional inference trees, taking into account three potential factors: corpus, text type and syntactic function. The results show that over time the complexity of the NP in Ghanaian English has increased, which is interpreted as Ghanaians becoming more proficient and therefore employing more sophisticated structures. Text type is an important predictor; syntactic function plays only a minor role. By adding a diachronic perspective, the study contributes to current models of the evolution of world Englishes.
Toxicological Profile and Pharmacokinetics of a Unilamellar Liposomal Vesicle Formulation of Amphotericin B in Rats
Authors: Boswell Garry W. , Bekersky Ihor , Buell Donald , Hiles Richard , Walsh Thomas J. 
Published: April 2020

ABSTRACT
AmBisome (ABLP) is a unilamellar liposomal preparation of amphotericin B that has demonstrated an improved safety profile compared to conventional amphotericin B. Single- and multiple-dose pharmacokinetics were determined by using noncompartmental methods for rats administered ABLP at 1, 3, 9, and 20 mg/kg/day. The toxicological profile was evaluated following 30 consecutive days of intravenous ABLP administration. Mean plasma amphotericin B concentrations reached 500 and 380 μg/ml (males and females, respectively) following 30 days of ABLP administration at 20 mg/kg. The overall apparent half-life was 11.2 ± 4.5 h (males) or 8.7 ± 2.2 h (females), and the overall clearance (CL) was 9.4 ± 5.5 ml/h/kg (males) or 10.2 ± 4.1 ml/h/kg (females). ABLP appears to undergo saturable disposition, resulting in a non-dose-proportional amphotericin B area under the curve and a lower CL at higher doses. Histopathological examination revealed dose-related transitional-cell hyperplasia in the transitional epithelium of the urinary tract (kidney, ureters, and urinary bladder) and moderate hepatocellular necrosis at the 20-mg/kg/day dose. Administration of ABLP in doses of up to 20 mg/kg/day resulted in 100-fold higher plasma amphotericin B concentrations, with significantly lower toxicity than that reported with conventional amphotericin B therapy.
Stereoselective Disposition of Sulbenicillin in Humans
Authors: Itoh Tomoo , Watanabe Naoko , Ishida Mariko , Tsuda Yasuyuki , Koyano Shinichi , Tsunoi Tomoko , Shimada Hideyo , Yamada Hideo 
Published: April 2020

ABSTRACT

Stereoselective disposition of sulbenicillin (SBPC) epimers in healthy human volunteers was studied in order to clarify the differences in pharmacokinetic behavior between the epimers. Stereospecific high-performance liquid chromatography was used for the determination of SBPC epimers. Plasma protein binding was measured in vitro with an ultrafiltration method. The binding was stereoselective, with the unbound fraction (

f
u

) of the
R
-epimer being approximately 1.3-fold greater than that of the
S
-epimer. SBPC was administered intravenously to human volunteers, and concentrations of SBPC in plasma and urinary excretion rates were measured. Renal clearance (CL
R
) for the unbound drug (approximately 400 ml/min) was greater than the glomerular filtration rate (GFR) (approximately 109 ml/min) for both epimers, suggesting that both epimers are secreted at the renal tubules. Renal tubular secretion appeared to be greater for the
S
-epimer. When probenecid was coadministered, the CL
R
values of both epimers were significantly reduced and were approximately equal to the GFR values. CL
R
was greater for the
S
-epimer (37.5 and 49.8 ml/min for
R
-SBPC and
S
-SBPC, respectively), which was simply due to the greater

f
u

of the
S
-epimer in plasma. In contrast, total body clearance was greater for the
R
-epimer (67.8 and 56.3 ml/min for
R
-SBPC and
S
-SBPC, respectively) because of the stereoselective degradation of the
R
-epimer in plasma. It was revealed that stereoselective degradation in the body had significant influence on the disposition of SBPC epimers.