bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cross-Species Complementation of Nonessential Yeast Genes Establishes Platforms for Testing Inhibitors of Human Proteins
Authors: Hamza Akil , Driessen Maureen R M , Tammpere Erik , O’Neil Nigel J , Hieter Philip 
Published: January 2020

AbstractGiven the broad utility of humanized yeast to model and study human biology, a reference set of human genes that can replace cognate yeast genes and operate in yeast is needed. Hamza et al. present...Cross-species complementation can be used to generate humanized yeast, which is a valuable resource with which to model and study human biology. Humanized yeast can be used as an in vivo platform to screen for chemical inhibition of human protein drug targets. To this end, we report the systematic complementation of nonessential yeast genes implicated in chromosome instability (CIN) with their human homologs. We identified 20 human–yeast complementation pairs that are replaceable in 44 assays that test rescue of chemical sensitivity and/or CIN defects. We selected a human–yeast pair (hFEN1/yRAD27), which is frequently overexpressed in cancer and is an anticancer therapeutic target, to perform in vivo inhibitor assays using a humanized yeast cell-based platform. In agreement with published in vitro assays, we demonstrate that HU-based PTPD is a species-specific hFEN1 inhibitor. In contrast, another reported hFEN1 inhibitor, the arylstibonic acid derivative NSC-13755, was determined to have off-target effects resulting in a synthetic lethal phenotype with yRAD27-deficient strains. Our study expands the list of human–yeast complementation pairs to nonessential genes by defining novel cell-based assays that can be utilized as a broad resource to study human drug targets.
ERRATUM - Revista FAMECOS - edição v. 26, n. 2 (2019) - ID 32229
Authors: Revista FAMECOS Equipe Editorial da 
Published: January 2020

A Equipe Editorial da Revista FAMECOS torna publica as seguintes alteração e substituição, na edição v. 26, n. 2 (2019), DOI: http://dx.doi.org/10.15448/1980-3729.2019.2 , ID 32229, artigo, Tempo, mídia e processos sociopolíticos no Brasil do século XXI: Perspectivas sociossemióticas.
A comunidade cientifica como sujeito epistêmico coletivo
Authors: Moreira Delvair Custódio 
Published: January 2020

Em epistemologia coletiva argumenta-se que grupos podem ser sujeitos epistêmicos. Ou seja, que grupos podem instanciar atitudes doxásticas, como crenças, passíveis de serem avaliadas como racionais, justificadas ou mesmo serem consideradas casos de conhecimento. Epistemólogos coletivos têm sustentado que grupos amplos como a comunidade científica como um todo também podem ser sujeitos epistêmicos. Mas não é óbvio que a comunidade científica seja capaz de atitudes doxásticas mesmo que o argumento que sustente a tese de que grupos têm tais atitudes for sólido. Isto, porque a tese de que grupos podem ser sujeitos epistêmicos depende de uma noção de compromisso conjunto que a comunidade científica parece não ser capaz de realizar. Seguindo essa linha de raciocínio objeta-se que a comunidade científica não é capaz de atitudes doxásticas e que a comunidade científica é incapaz de formar um compromisso conjunto. Dada as objeções, a comunidade científica não poderia ser tratada como um sujeito epistêmico. Meu objetivo neste trabalho é responder a essas objeções e defender que a comunidade científica pode ser um sujeito epistêmico.
Heidegger e Paulo. Contração da temporalidade e religiosidade “escatológica” na situação do hos me paulino (1 Cor 7, 29-31)
Authors: Silva Santos Bento 
Published: January 2020

O artigo destaca a noção de temporalidade com base na interpretação fenomenológica do texto paulino de 1 Cor 7,29-31 na segunda parte da preleção friburgense do semestre de inverno de 1920/1921 intitulada “Introdução à Fenomenologia da Religião (GA 60, 119-121). Heidegger volta-se para o que unicamente faz do cristianismo um evento originariamente histórico, a saber: a fé cristã. Dentre os três fenômenos temporalmente eminentes presentes na segunda parte do curso (o ter-se-tornado [Gewordensein], o “como se não” [alsobnicht, hōsmē] e a espera da parusia) destacamos a partícula grega hōs mē de 1 Cor 7,29-31 como atuação autêntica do presente: a fórmula hōs mē não tem somente um sentido negativo, mas para Paulo o “como se não” significa que o comportamento induzido pelo viver “como se não” não exclui o engajamento no mundo circundante (Umwelt) e mais precisamente contra ele. Explicitar esse “retorno” à situação fática que caracteriza a vida cristã tal como Paulo a viveu através da escrita de suas epístolas implica examinar, sucessivamente, os dois momentos fundamentais da fenomenologia do jovem Heidegger (1), o texto paulino a partir da relação entre concentração e tensão escatológicas (2), a fórmula hōs mē e o complexo realizador da vida cristã com base no horizonte escatológico (3) e, a guisa de conclusão, a relação entre “concepção prévia” e facticidade cristã com base na coesão estrutural entre religiosidade cristã originária, experiência fática da vida e temporalidade(4).
Dependência epistêmica, testemunho e gettierização
Authors: Fett J.R. 
Published: January 2020

O objetivo deste ensaio é examinar a proposta de Sandy Goldberg, segundo a qual há divisão de trabalho epistêmico em certos processos de aquisição de conhecimento – ao menos em se tratando de conhecimento testemunhal. Goldberg propõe mostrar a veracidade desta alegação salientando a nossa dependência epistêmica em relação a outros indivíduos, ou mesmo comunidades inteiras. Nós, então, vamos propor o tratamento de um famoso caso tipo-Gettier que, segundo Gilbert Harman, revelaria algumas dimensões sociais do conhecimento. Por fim, nós esperamos tirar algumas lições da explicação oferecida por Goldberg, dentre as quais a mais importante a ser tomada é que, havendo tal divisão de trabalho epistêmico em processos de aquisição de conhecimento, muitas vezes, não são apenas as propriedades epistêmicas do sujeito que determinam a sua posse de conhecimento ou ignorância, de modo que o conhecimento é mais social do que individual em se tratando de uma ampla gama de casos.
Sobre a teoria do conhecimento nas Passagens de Walter Benjamin - sujeito em desagregação
Authors: De Mattos Manuela Sampaio 
Published: January 2020

O presente artigo apresenta a distinta teoria do conhecimento proposta por Walter Benjamin em seu projeto das Passagens. Tal proposta já encontra suas origens em seus textos de juventude, principalmente em seu Sobre o programa de uma filosofia vindoura. Levando isso em conta, articulamos os dois momentos da obra de Benjamin para demonstrar que esta nova teoria materialista, dialética e imagética do conhecimento pressupõe um conceito mais alto de experiência, assim como uma crítica contundente ao sujeito do conhecimento que se encontra em vias de desagregação. Para nos aprofundarmos na proposição benjaminiana, adentramos a importante topografia conceitual presente nas Passagens, passando pelos temas da linguagem, do sonho, do despertar e das imagens dialéticas.
Sobre a natureza da inferência em Boghossian
Authors: Engel Doraci 
Published: January 2020

Se crer com base em razões é algo que aparentemente envolve passos e toma tempo o que nos previne de considerar o julgamento inferencial ou o raciocínio de um modo geral como uma atividade, o produto de um tipo especial de agência? A ideia de que há atividade genuína quando realizamos uma inferência está implícita em vários autores. Neste artigo analiso uma defesa explicita desta concepção: a tese desenvolvida por Paul Boghossian de que a inferência é uma forma de ação cognitiva. Argumento que há um contraste na estrutura das razões e de causação entre crer por razões e agir por razões. E concluo que o que parece ser ativo no caso especifico de raciocínio, que é fazer uma inferência, deve-se a sua conexão conceitual com o estado de crença. Quando cremos que p com base em q, cremos por uma razão que nos é dada por uma outra crença.
Bioethica de las verdades y la excepcion imanente: Sobre política, matemáticas y ética en el sistema filosófico de Alain Badiou
Authors: Madarasz Norman Roland 
Published: January 2020

El prestigio académico adquirido por la bioética en el último medio siglo se debe en parte al fortalecimiento de los espacios de libertad social en las democracias representativas. En el contexto de la ética, se creó la noción de libertad, pero sin las formas legalizadas de libertad, la ética no puede crecer como fuente creativa de las normas por las cuales se formulan las múltiples formas de juicio moral. En esta época de la post-verdad y de la producción estratégica de mentiras y del odio políticos, el efecto sobre los sujetos políticos parece indicativo de carencias psicológicas, educativas y especialmente económicas. En vista de este escenario, es importante reconsiderar el alejamiento de la teoría de la verdad como un “filtro” de los discursos y las orientaciones políticas producidas por la ética. La tarea que se plantea a la filosofía es extender la fuerza conceptual y discursiva de la bioética para contemplar formas de subjetividad en ruptura con una estructura de estado que produce injusticia. Para ello, analizamos críticamente la propuesta de una bioética de verdades, un filtro separador en el sistema de Alain Badiou, que organiza la relación entre ontología, fenomenología y las condiciones históricas de producción de verdades.
Nonmetallic inclusions in rails made of electro-steel alloyed with chromium
Authors: Umanskii A. A. , Golovatenko A. V. , Simachev A. S. 
Published: January 2020

Type, relative concentration and size of most characteristic non-metallic inclusions for the elements of rails (head, web) from electro-steel of E79KhF and E90KhАF grades were determined on the basis of metallographic (with a microscope “OLYMPUS GX-51”) and spectral (using spectrometer “ARL iSpark” method “Spark-DAT”) analyses. It was found that the highest relative concentration of manganese sulfides (MnS) is 30.8 – 43.4 ppm. At the same time, 60 – 100 % of inclusions of this type are of small sizes (less than 4 μm), and it does not allow them to be detected using standard metallographic analysis with 100-fold magnification. The revealed high relative concentration of sulfide inclusions directly correlates with the established positive sulfur liquation in considered rail elements, which is up to 40 %. Despite the high concentration of manganese sulfides, their influence on the quality of rails can be considered not dangerous, taking into account their high ductility during hot deformation and the established prevalence of inclusions of this type with small size (less than 4 μm). Among inclusions of a silicate type, SiO2 inclusions (3.4 – 14.9 ppm) have a significant concentration. All detected inclusions of this type have a size not exceeding 4 μm. It was found that the concentration of complex inclusions containing alumina (Al2O3 – CaO – MgO, Al2O3 – CaO – MgO – CaS, Al2O3 – CaO, Al2O3 – MgO) is insignificant: in total it does not exceed 3.1 ppm and 1.6 ppm for individual types. The concentration of corundum (Al2O3) is also insignificant and does not exceed 0.3 ppm. In this case, alumina inclusions of small size (less than 6 μm) prevail. Due to the low contamination (taking into account the relative concentration and size of inclusions) with non-plastic silicate and alumina non-metallic inclusions, their influence on the quality of the rails was not significant. It is confirmed by the absence of defects detected during ultrasonic testing.
Analysis of the Existence of Signage in Batik Lasem Tourism Village
Authors: Mandaka Mutiawati 
Published: January 2020

Research on the analysis of the presence of signage in the Lasem Batik Tourism Village aims to identify signage, activities and visual areas of the Lasem written batik area, identify visual forming factors in the Lasem written batik area and get an analysis of the presence of signage in the Lasem batik tourism village area and provide recommendations on the results of the analysis so that they can provide a reference and guide for local governments/managers in carrying out the stages of developing a new tourist attraction vehicle in Rembang district.This study uses quantitative rationalistic methods, using regression analysis techniques to look for significant signage influence. Stratified random sampling is used to achieve objectivity. The population used as respondents are people with an educational background in architecture, the general public and residents who live there every day. Furthermore, the number of samples taken proportionally, while the sample area is determined using the area sampling technique. This method was taken in accordance with the purpose of the study to analyze the presence of signage in the Lasem has written batik tourism village area. Based on the findings it can be concluded that the presence of signage in commercial buildings/showrooms has a significant positive effect on the visual aesthetic of Lasem Batik Tourism Village. Keywords: Signage, Visual Aesthetics, Lasem Batik Tourism Village
Conservation of Cultural Heritage Architecture and Development of Tourism in Denpasar, Bali
Authors: Runa I Wayan , Warnata I Nyoman , Anasta Putri Ni Putu Ratih Pradnyaswari 
Published: January 2020

This research aims to examine the role of conservation of cultural heritage architecture in the development of tourism in Denpasar and to describe the concept that shapes the downtown Denpasar (zone Z). Following the characteristics of the object of study (research questions), this research uses phenomenology as the paradigm. The qualitative method (phenomenology) is an inclusive and flexible method, which is always developing. This method helps to gain an understanding of meaning, develop theories, and describes complex realities. This research was conducted with the help of human instruments (the researchers), notebooks, voice recorders, and research assistants. Additionally, this research emphasizes qualitative techniques, in which researchers or human factors have an important role. Primary data consist of words and actions, while secondary data include documents, photos, and statistics. Results show that the cultural life of Denpasar citizen has a significant effect on the development of tourism in Denpasar, Bali. It is evident in the structure, functions, and meaning of downtown Denpasar Heritage City, especially in managing the area, planning, and building layout. The downtown Denpasar has a unique pattern called Pempatan Agung. In the context of tourism, the development of Denpasar should focus on creativity and culture. The responsibility of managing the area should be shouldered by the community, cultural experts, universities, NGOs, private companies, and the government. Also, there needs to be a continuous evaluation of the results of management. Keywords: Pempatan Agung, culture, creativity.
Consistómetro Bostwick como una herramienta visual de control de calidad para alimentos espesados
Authors: MIRANDA ZAMORA William Rolando , CALLE BERRU Estrellita Mayra , FLORES MENDOZA Lesly Carolina , IPANAQUE PULACHE Daly Marcela , LOPEZ BURGOS Luis Vidal , SÁNCHEZ CHERO Manuel Jesús , SÁNCHEZ CHERO José Antonio 
Published: January 2020

Diseñar, construir y medir la consistencia Bostwick de alimentos espesados a un nivel objetivo, ya que la consistencia Bostwick es crítica para el control de calidad de los mismos. Este estudio examinó la relación de la extensión o recorrido en el Bostwick (es decir, la distancia que fluye un líquido) para una variedad de alimentos que se expenden en el Mercadillo de Sullana y se determinó si la distancia de flujo diferenciaba visualmente el espesor de alimentos versus la consistencia similar a la miel. Se probaron 4 productos espesados (mostaza, aderezo, mayonesa y miel) adquiridos del Mercadillo de Sullana. Las mediciones se registraron usando el consistómetro Bostwick. Las consistencias Bostwick de los alimentos espesados y parecidos a la miel diferían significativamente en su grado de recorrido. Usando nuestro consistómetro Bostwick, B10s: 13 cm en la miel (83,95°Brix), B30s: 2,2 cm en mostaza (12,3°Brix), B10s: 4,3 cm en mayonesa (0,25°Brix) y aderezo (7,5°Brix) que no se logró realizar debido a su consistencia (muy liquida, consistencia similar agua), ninguno de los alimentos medidos tiene consistencia similar a la miel. Hubo una correlación inversa entre los datos de la viscosidad y los resultados de la consistencia Bostwick.
Palabras clave: Consistencia, consistómetro, alimentos espesados.
Socioenvironmental networks and international cooperation: The Global Alliance for Recycling and Sustainable Development- GARSD
Authors: Ziglio Luciana Aparecida  Iotti , Ribeiro Wagner Costa 
Published: January 2020

Networks of nongovernmental organizations dedicated to socio-environmental issues have emerged in recent decades. The involvement of these networks in the international sphere has enabled new approaches to international cooperation. This article introduces the concept of international cooperation and the Global Alliance for Recycling and Sustainable Development (GARSD), a socio-environmental network created by a coalition of NGOs dedicated to promoting recycling of municipal solid waste. This article is the result of a doctoral study conducted using documentary analysis, participant observation and other qualitative data collection methods. The study conclusively finds that it is neessary to analyze the work of these non-state actors within the context of international cooperation between nation states and the possibility of (re)creating political-territorial arrangements, such as networks comprising a variety of actors especially geared towards socio-environmental issues.
Identification Prevalence of Musculoskeletal Disorders and its Related Factors among Street Cleaners of Isfahan Municipality
Authors: Abbaspour MohammadReza , Habibi Ehsan 
Published: January 2020

Background: Musculoskeletal disorders are one of the major work-related diseases resulting from repetitive motions, awkward postures and exertion of excessive force on joints or an acute trauma. The present study was an attempt to determine the Prevalence and factors associated with Musculoskeletal Disorders among street cleaners of Isfahan Municipality. Methods: the present article is a cross-sectional causal-comparative study conducted on street cleaners of Isfahan Municipality. Cochran's formula was used to select 173 individuals as samples through convenience sampling. The data collection instrument was a researcher-made questionnaire covering demographic data (age, marital status, previous and current work experience, type of physical activity, work-related activity, height and weight of individuals, height and weight of tools, etc.) and specialized data (Nordic Self-Reporting Questionnaire). SPSS software and independent T-test were used to analyze the collected data. Results: 93% of street cleaners experienced musculoskeletal disorders in at least one organ over the past 12 months and factors associated with these disorders reportedly include occupational experience, age, marital status, height and weight of instrument, Body mass index and posture). The musculoskeletal disorders were found to be most prevalent in lower back (33%) and the shoulders (32.9%) and least prevalent in thighs (5%) over the past 12 months. Conclusion: The results showed that musculoskeletal disorders are highly prevalent in different parts of street cleaners’ body, and a variety of factors including work experience, age, marital status, tool height, tool weight, tool grip strength and posture are associated with that. The results also showed that correction of body movements and designing an ergonomic tool commensurate with the physical activity of street cleaners can prevent musculoskeletal disorders among them.
Tranzycyjny efekt jojo w sekwencjach społecznych młodych migrantów
Authors: Winogrodzka Dominika , Sarnowska Justyna 
Published: January 2020

Wydłużająca się edukacja czy uelastycznienie się rynków pracy powodują, że poszczególne etapy wczesnodorosłego życia młodych ludzi oraz podejmowane role społeczne przenikają się (Wyn, Dwyer 2002; Mayer 2005; Biggart, Walther 2006; Kohli 2007; Du Bois-Reymond 2009; Heinz 2009; Mary 2014). Młodzi ludzie wchodzą w dorosłość coraz częściej w sposób nielinearny, doświadczając powrotów do etapów czy sytuacji z przeszłości, co w literaturze zostało opisane jako „tranzycja jojo” (Du Bois-Reymond, López Blasco 2003; Heinz 2009; Walther 2009; Hörschelmann 2011; Borlagdan 2015). „Jojoizacja tranzycji” dotyczy również procesu przechodzenia z edukacji do rynku pracy, które jest coraz bardziej rozciągnięte w czasie, zróżnicowane i trudne do przewidzenia (Walther 2006). Jednym z istotnych zjawisk towarzyszących inicjacji zawodowej współczesnych młodych dorosłych jest mobilność międzynarodowa (King 2018; Robertson, Harris, Baldassar 2018), która znacznie wpływa na kształt ścieżek edukacyjno-zawodowych młodych ludzi.Celem artykułu jest odpowiedź na pytanie o rolę migracji zagranicznych w występowaniu tranzycyjnego efektu jojo. Na bazie studiów przypadku ścieżek edukacyjno-zawodowych migrantów przedstawione zostaną tranzycje ludzi młodych na rodzimy i zagraniczny rynek pracy, zarówno w wymiarze obiektywnym, jak i subiektywnym. Jako metoda analizy posłuży jakościowa adaptacja analizy sekwencji społecznych (Cornwell 2015).
Enhancing Autonomy through the Occupational Engagement of Adults with Intellectual Disabilities: Supported Employment Model Applied by the Polish Association for Persons with Intellectual Disabilities (Branch in Zgierz)
Authors: Mikołajczyk-Lerman Grażyna , Potoczna Małgorzata 
Published: January 2020

Reflections presented in this paper reverberate with the current social discourse based on respect for human rights, with heavy emphasis put on the active policy of employment of persons with disabilities. Improvement of the employment support system seems to be one of the most efficient forms of integrating this previously excluded and marginalized group of people into the mainstream social life. It is especially important in the case of persons with intellectual disabilities, who often remain in a particularly difficult situation for many reasons. Enhancing their autonomy via occupational engagement may be beneficial both at the individual (improved personal functioning) and macro (reduced costs for the state and welfare system) levels. The authors of this paper reflect upon the development and implementation of best practices in terms of building an effective mechanism of promoted social participation and the occupational engagement of persons with intellectual disabilities in an open social environment. This seems to create a real chance for community integration, to which these persons have the inalienable right, although so far they have often been left out of the mainstream social life.
Między światem realnym a wirtualnym: obietnice vs. rzeczywistość. Prekarna praca kierowcy Ubera?
Authors: Polkowska Dominika 
Published: January 2020

Pracę za pośrednictwem aplikacji mobilnych można uznać za klasyczne realizowanie idei elastyczności. Firma Uber jako pionier w obszarze gospodarki platformowej zdaje się potwierdzać tę prawidłowość składanymi przez siebie obietnicami. Nie zawsze jednak punkt widzenia dostarczyciela technologii pokrywa się w praktyce z oczekiwaniami jego użytkowników. Celem artykułu jest odpowiedź na pytanie, czy obietnice składane przez Ubera mają pokrycie w rzeczywistości oraz czy elastyczność i pozorna autonomia obiecywane kierowcom prowadzą do wzrostu niepewności pracy w kontekście pracy prekarnej. Podstawą rozważań jest analiza postów zamieszczanych w zamkniętych grupach dyskusyjnych na jednym z portali społecznościowych – grupujących kierowców Ubera, a także indywidualnych wywiadów pogłębionych zrealizowanych wśród samych kierowców zgodnie z metodologią teorii ugruntowanej.