bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hemodynamic changes in superficial arteriovenous malformation surgery measured by intraoperative ICG fluorescence videoangiography with FLOW 800 software
Authors: Ye Xun , Wang Liang , Li Ming-tao , Chen Xiao-lin , Wang Hao , Ma Li , Wang Rong , Zhang Yan , Cao Yong , Zhao Yuan-li , Zhang Dong , Wang Shuo 
Published: March 2020

Abstract
To analyze the application of intraoperative indocyanine green (ICG) angiography with FLOW 800 software in arteriovenous malformation (AVM) surgeries. Data on 17 patients undergoing surgery with ICG fluorescence were collected. To analyze the hemodynamic features of AVM and the influence on the peripheral cortex of AVM resection, we assessed the following hemodynamic parameters: maximum intensity, slope of rise, time to half-maximal fluorescence, and transit time from arteries to veins. In the 17 superficial AVMs studied, the time delay color mode of FLOW 800 software was superior to the traditional playback mode for identifying feeding arteries, draining veins, and their relation to normal cortical vessels.The maximum fluorescence intensity and slope of the ICG fluorescence curve of feeder arteries and draining veins were higher than those of normal peripheral vessels (P < 0.05). The transit times in AVMs were significantly shorter than those in normal peripheral vessels (P < 0.05).After AVM resection, cerebral flow increased in the cortex, and local cycle time become longer, although the differences were not significant (P > 0.05). Hemodynamic parameter analysis provided quality guidance for the resection of AVMs and could also be used in estimating changes in blood flow in the local cortex to identify abnormal hyperperfusion and residual nidus.
Construction and investigation of lncRNA-miRNA-mRNA ceRNA network in thymic epithelial neoplasms
Authors: Tang Hanxiao , Ge Shuyu , Sun Pingping 
Published: March 2020

Abstract
Background Competing endogenous RNA (ceRNA) networks may be used to relate the functions of protein-coding mRNAs with those of the non-coding RNAs, such as microRNAs (miRNAs) and the long non-coding RNAs (lncRNAs). ceRNAs enable the post-transcriptional regulation of gene expression by competing for the shared miRNAs. However, the role and function of the lncRNA-miRNA-mRNA ceRNA network in thymic epithelial neoplasms (TEN) remains unknown. Methods The miRNA, mRNA, and lncRNA expression profiles of 124 patients with TEN were downloaded from The Cancer Genome Atlas. We identified the differentially expressed (DE) miRNAs, mRNAs, and lncRNAs using the limma package in R software. The GDCRNATools package was used for the Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway annotations. Cytoscape software was used to construct the lncRNA-miRNA-mRNA ceRNA network. The Gene Expression Profiling Interactive Analysis platform was used to estimate the overall survival (OS) rates of the patients. Survival curves were analyzed using the log-rank test. Finally, the mRNAs in the ceRNA network were analyzed using the GOplot package in R. Results A total of 1513, 188, and 579 TEN-specific mRNAs, lncRNAs, and miRNAs, respectively, were identified. The lncRNA-miRNA-mRNA ceRNA network was constructed, and included 53 mRNAs, 4 lncRNAs, and 27 miRNAs. A total of 10 DEmRNAs (DLX2, C8orf88, CD38, GATA3, MAL, FOXQ1, FOLH1, NLRP12, HJURP, and ACSM1) and 1 lncRNA (SNHG3) were found to be significantly associated with OS ( P <0.05). Conclusion In this study, we constructed a lncRNA-miRNA-mRNA ceRNA gene regulatory network for TEN, and identified potential prognostic and diagnostic biomarkers, as well as therapeutic targets, for the disease.
“The right time is just after birth”: Acceptability of point-of-care birth testing in Eswatini: qualitative results from infant caregivers, health care workers, and policymakers
Authors: Sacks Emma , Khumalo Philisiwe , Tsabedze Bhekisisa , Montgomery William , Mthethwa Nobuhle , Nhlabatsi Bonisile , Masuku Thembie , Cohn Jennifer , Chouraya Caspian 
Published: March 2020

Abstract
Background: Testing for HIV at birth has the potential to identify infants infected in utero , and allows for the possibility of beginning treatment immediately after birth; point of care (POC) testing allows rapid return of results and faster initiation on treatment for positive infants. Eswatini piloted birth testing in three public maternities for over two years. Methods: In order to assess the acceptability of POC birth testing in the pilot sites in Eswatini, interviews were held with caregivers of HIV-exposed infants who were offered birth testing (N=28), health care workers (N=14), and policymakers (N=10). Participants were purposively sampled. Interviews were held in English or SiSwati, and transcribed in English. Transcripts were coded by line, and content analysis and constant comparison were used to identify key themes for each respondent type. Results: Responses were categorized into: knowledge, experience, opinions, barriers and challenges, facilitators, and suggestions to improve POC birth testing. Preliminary findings reveal that point of care birth testing has been very well received but challenges were raised. Most caregivers appreciated testing the newborns at birth and getting results quickly, since it reduced anxiety of waiting for several weeks. However, having a favorable experience with testing was linked to having supportive and informed family members and receiving a negative result. Caregivers did not fully understand the need for blood draws as opposed to tests with saliva, and expressed the fears of seeing their newborns in pain. They were specifically grateful for supportive nursing staff who respected their confidentiality. Health care workers expressed strong support for the program but commented on the high demand for testing, increased workload, difficulty with errors in the testing machine itself, and struggles to implement the program without sufficient staffing, especially on evenings and weekends when phlebotomists were not available. Policymakers noted that there have been challenges within the program of losing mothers to follow up after they leave hospital, and recommended stronger linkages to community groups. Conclusions: There is strong support for scale-up of POC birth testing, but countries should consider ways to optimize staffing and manage demand.
Whole-body Vibration Attenuates Pyroptosis-Mediated Inflammation but Accelerates Progression of Intervertebral Disc Degeneration
Authors: Fu Fang-da , Yao Sai , Sun Zhi-tao , Zhou Cheng-cong , Yu Huan , Luo Huan , Pan Zhiyong , Jin Hong-ting , Tong Pei-jian , Chen Di , Wu Cheng-liang , RUAN Hongfeng 
Published: March 2020

Abstract
Background Whole body vibration (WBV) is a non-pharmaceutical therapy that has been widely incorporated into clinical practice for musculoskeletal disorders, including low back pain (LBP). Intervertebral disc (IVD) degeneration (IVDD) is clinically associated with LBP and is known as the main cause for LBP. However, cumulative evidence also suggested WBV might have an adverse impact on IVDs. Moreover, previous studies have been focusing on the effects of WBV on healthy mice, rather than those suffering from IVDD. Thus, uncertainties still exist concerning the effects of WBV on IVDs undergoing IVDD. This study was aiming to evaluate the effects of WBV intervention on the development and progression of IVDD mouse model induced by lumbar spine instability (LSI) surgery. Methods LSI surgery, by resecting the lumbar 3 rd -5 th spinous processes along with the supraspinous and interspinous ligaments, was conducted in 10-week-old male mice which then received WBV treatment (1 h per day, 5 days per week, at 3 Hz with peak acceleration at 0.4 g) or sham treatment. The progression of IVDD was evaluated by MRI, μCT and histological analyses after WBV treatment. The matrix metabolism, distribution of sensory nerves, pyroptosis in IVDs tissues were determined by immunohistological analysis or real-time PCR. The apoptosis of IVD cells was detected by TUNEL assay. Results LSI surgery was successful in producing IVDD modeling. WBV caused decreases in IVD height and annulus fibrosus (AF) score, as well as increased numbers of apoptotic cells in IVD tissues. WBV contributed to sensory innervation into AF and upregulation of Adamts5 and MMP3 expression in IVDD mice received LSI surgery. In addition, WBV treatment triggered earlier activation of Wnt/β-catenin signaling in IVDD mice with WBV treatment compared with those without WBV treatment. Unexpectedly, WBV significantly attenuated Caspase-1 and IL-1β expression in AF. Conclusions Collectively, our findings demonstrate that WBV treatment may worsen the development of ongoing IVDD. Decrease of IL-1β expression after WBV intervention may partially account for patient self-reported pain relief after WBV treatment in some previous trials. This study may help us better understand the effects of WBV intervention on patients experiencing LBP resulting from the degeneration of lumbar IVDs.
Long non-coding RNA FAM83H-AS1 acts as an oncogenic driver in human ovarian cancer
Authors: Yuan Xiaolei , Huang Ying , Guo Man , Hu Xiaowei , Li Peiling 
Published: March 2020

Abstract
Background Ovarian cancer (OC) is one of the most aggressive women cancers with increasing incidence and mortality rates worldwide. Long non-coding RNAs (lncRNAs) could as major players in OC process. Although FAM83H antisense RNA1 (FAM83H-AS1) is demonstrated play an important roles in a many cancers, the detailed function and mechanism has not been reported in OC. Methods We integrated multiple kinds of bioinformatics approaches and experiments validated method to evaluate functions of FAM83H-AS1 in OC. Results Some differential expressed lncRNAs were identified between OC and normal control tissues. FAM83H-AS1 was one of most differentially expressed lncRNAs and up-regulated in multiple cancer types. Specially, expression of FAM83H-AS1 was higher in OC and showed difference in diverse stages. High FAM83H-AS1 expression is associated with worse pan-cancer and OC outcomes. FAM83H-AS1-centric network including lncRNA-miRNA, lncRNA-protein and lncRNA-mRNA ceRNA network were constructed to infer the function and mechanism of FAM83H-AS1. There were two methylation sites including cg01399317 and cg20519035 located at FAM83H-AS1. The methylation level of cg01399317 was correlated with gene expression of FAM83H-AS1. The expression level of FAM83H-AS1 was correlated with infiltration level of immune cell including macrophage, neutrphil and dendritic cell in OC patients. Lastly, qRT-PCR showed that the expression of FAM83H-AS1 was higher in OC tissues than normal control tissues. Conclusions Collectively, these results indicated that FAM83H-AS1 may act as an oncogenic driver and it may be a potential therapy target in OC.
Stereotactic Body Radiation Therapy Mitigates Radiation Induced Lymphopenia in Early Stage Non-Small Cell Lung Cancer
Authors: McLaughlin Mark , Alam Morshed , Smith Lynette , Ryckman Jeffrey , Lin Chi , Baine Michael J. 
Published: March 2020

Abstract
Background Radiation-induced lymphopenia (RIL) occurs during treatment with conventional radiation in multiple organ sites. Development of RIL portends poor prognosis. Stereotactic body radiation therapy (SBRT) spares RIL in pancreatic cancer, but has not been examined in other sites commonly treated with SBRT. This work examines if SBRT similarly spares RIL in patients with non-small cell lung cancer (NSCLC). Methods Retrospective analysis was done at a single institution on 40 distinct cases of SBRT for early stage NSCLC from 2006-2017. Incidentally collected lymphocyte counts collected within 6 months of SBRT treatment were analyzed to determine if RIL occurred. The presence of RIL was correlated with location of initial failure and survival endpoints. Kaplan-Meier curves were constructed with significance defined at the level p = 0.05. Results RIL was observed in 35% of the analyzed patients. Patterns of failure and survival data were comparable to prior SBRT literature. There was no observed association in two year local, nodal, or distant failure, progression free survival, or overall survival based on the presence of RIL. Conclusions SBRT spares RIL in NSCLC compared to historical rates observed with conventionally fractionated radiation. As understanding of the role of the immune system in cancer control continues to evolve, the importance of RIL sparing techniques take on increasing importance. This study represents the first analysis of RIL sparing in SBRT in an early stage NSCLC cohort without the confounding influence of chemotherapy.
Motor unit number index detects the effectiveness of surgical treatment in improving distal motor neuron loss in patients with incomplete cervical spinal cord injury
Authors: Li Jun , Zhu Yancheng , Li Yang , He ShiSheng , Wang Deguo 
Published: March 2020

Abstract
Background Recovery of motor dysfunction is important for patients with incomplete cervical spinal cord injury (SCI). To enhance the recovery of muscle strength, both research and treatments mainly focus on injury of upper motor neurons at the direct injury site. However, accumulating evidences have suggested that SCI has a downstream effect on the peripheral nervous system, which may contribute to the poor improvement of the muscle strength after operation. The aim of this study is to investigate the impact of early vs. delayed surgical intervention on the lower motor neurons (LMNs) distal to the injury site in patients with incomplete cervical SCI. Methods Motor unit number index (MUNIX) was performed on the tibialis anterior (TA), extensor digitorum brevis (EDB) and abductor hallucis (AH) in 47 patients with incomplete cervical SCI (early vs. delayed surgical-treatment: 17 vs. 30) and 34 healthy subjects approximately 12 months after operation. All patients were further assessed by American spinal injury association (ASIA) motor scales and Medical Research Council (MRC) scales. Results There are no difference of both ASIA motor scores and MRC scales between the patients who accepted early and delayed surgical treatment (P > 0.05). In contrast, the patients undergoing early surgical treatment showed lower MUSIX values in both bilateral EDB and bilateral TA, along with greater MUNIX values in both right-side EDB and right-side TA, compared to the patients who accepted delayed surgical treatment (P < 0.05). Conclusions Cervical SCI has a negative effect on the LMNs distal to the injury site. Early surgical intervention in Cervical SCI patients may improve the dysfunction of LMNs distal to the injury site, reducing secondary motor neuron loss, and eventually improving clinical prognosis.
Antegrade elastic stable intramedullary nailing in treatment of irreducible distal radius fractures in diaphyseal-metaphyseal junction in children
Authors: Lin Weifeng , Liu Shuai , Hui Taotao 
Published: March 2020

Abstract
Purpose This paper aimed to study the method and outcome of antegrade elastic stable intramedullary nailing (ESIN) for irreducible distal radius fractures in diaphyseal-metaphyseal junction in children. Methods 27 irreducible distal radius fractures in children ranging in age from six to 13 years treated with closed reduction and antegrade ESIN from March 2017 to September 2018 were analyzed retrospectively. The series comprised 24 boys and three girls. 17 cases were double fractures in forearms and ten cases were single fractures of radius. Each case was treated with closed reduction, internal fixation of antegrade ESIN and plaster cast. ESIN was removed when fracture healed and remodelled well. Cooney modified Green-O'Brien wrist score was used to evaluate the wrist function. Ranges of motion (ROM) in bilateral wrists were contrasted at the final follow-up. P<0.05 was considered statistically significant in the difference. Results All the fractures healed four to six weeks post-operative. At the final follow-up (12-16 months), there was no statistical significance in ROM of bilateral wrists (all p>0.05). The mean Cooney modified Green-O'Brien wrist score was 93 (80-100) including 25 excellent (93%) and two good (7%) results. Conclusions The technique of antegrade ESIN is effective and minimal invasive for irreducible distal radius fractures in children. The criterion of proximal radius approach to implant ESIN should be studied further in minimal invasive therapy of fractures.
How do parents deal with their children’s chronic kidney disease? A qualitative study
Authors: Khorsandi Fatemeh , Parizad Naser , Feizi Aram , MaslakPak Masumeh Hemmati 
Published: March 2020

Abstract
Background: Parents’ adaptation has an impact on the health outcomes of children with chronic kidney diseases (CKD). Identifying factors that affect parents’ adaptation is necessary to fully understand their adaptation status. This study aims to explore parents’ adaptation with their child chronic disease and to determine related factors.Methods: This was a qualitative study with a content analysis approach. Sixteen parents of children with CKD were selected by using the purposive sampling. The nursing PhD candidate performed semi-structured in-depth face-to-face interviews to collect data. Conventional content analysis was used to analyze data.Results: Two main categories were extracted from the data were “adaptation facilitators” and “adaptation barriers”. Adaptation facilitators supported by three sub-categories: “social support”, “positive aspects of life”, “spiritual beliefs”. Five sub-categories of “adaptation barriers” were revealed as follows: “parents’ confusion”, “the disease related issues”, “pressures from peers and acquaintances”, “inappropriate treatment conditions” and “children and family limitation”.Conclusions: Two types of factors that influence parents’ adaptation were identified. The treatment team must evaluate these factors to help parents to adapt to their child’s illness by identifying and eliminating the adaptation barriers, and identifying and using the adaptation facilitators.
Transcriptome analysis reveal the mechanism of susceptible wheat seedlings response to Puccinia striiformis f. sp.
Authors: Liu Rong , Chen Yuwei , Zhou Min , Lu Jing , Zhang Chihong , Zhang Lei , Wu Yu 
Published: March 2020

Abstract
Background Wheat (Triticum aestivum L.) is most widely cultivated and a major staple food crops in the world. Stripe rust caused by Puccinia striiformis f. sp. tritici (Pst), which significantly reduce yield and quality of wheat. Although some resistant genes have been successfully used in wheat breeding, large of the regulating networks and the underlying molecular mechanisms of Pst response remains unknown. Therefore, to identify differentially expressed genes (DEGs) and regulate network involved in Pst resistance, we sequenced 15 cDNA libraries constructed from wheat seedlings with CYR34 infection.Results In this study, a highly susceptible cv. Chuanyu12 (CY12) were used to study the transcriptome profiles after inoculated with Pst physiological race CYR34. A total of 13892, 10195, 12268 and 14044 DEGs were investigated at 24h, 48h, 72h and 7days Pst infection, respectively. Certain key genes and pathways responsible for Pst-CYR34 in CY12 were identified. The results revealed that Pst-CYR34 inhibited the DEGs related to energy metabolism, biosynthesis, carbon fixation, phenylalanine metabolism, and plant hormone signaling pathway after Pst inoculation at 24h, 48h, 72h and 7d. These down-regulated DEGs including light-harvesting chlorophyll protein complex in photosystem I and photosystem II; cytochrome b6/f/ complex, F-type ATPase and photosynthetic electron transport; ethylene, jasmonic acid (JA) and salicylic acid (SA); lignin and flavonoids biosynthesis in CY12. Quantitative Real-time PCR analysis verified the expression patterns of these DEGs.Conclusions Our results give insights into the foundation for further exploring the molecular mechanism regulating networks of Pst response and pave the way for durable resistant breeding in bread wheat.
Comparison of transcriptomic and proteomic analyses to construct a model for the promotion of starch synthesis by ABA in Euryale ferox Salisb. seeds
Authors: Wu Peng , Li Xiang , Liu Xian , Xu Xu , Zhang Yu , Li Liangjun 
Published: March 2020

Abstract
Background: Euryale ferox Salisb. is an annual aquatic herb and the only species belonging to the genus Euryale in the Nymphaeaceae family. E. ferox seeds are used in medicine and diets. Starch is the main factor affecting E. ferox seed quality, but its regulatory mechanism has not been elucidated. Results: Herein, four time points of seed development, including after flowering T1 (10 days), T2 (20 days), T3 (30 days) and T4 (40 days), were investigated by using RNA-Seq and iTRAQ technology. Using weighted gene co-expression network analyses (WGCNAs), co-expressed genes and hub genes were identified for each module. Of particular importance are the discoveries of specific modules for seed starch during the seed developmental stages. The candidate regulators of seed starch are involved in the hormonal signaling pathways. Three ABA signaling receptor kinases, EfPYR1, EfSnRK2.1 and EfSnRK2.2, were identified as hub genes functioning in starch synthesis during the seed maturation process. The changes in expression pattern, ABA and starch content also indicated that ABA is positively correlated with starch. Conclusions: Together, these results indicate that E. ferox seed accumulation of starch is promoted by ABA, providing new insights into the regulatory mechanism of starch synthesis in E. ferox seeds.
Understanding the problem of access to public health insurance schemes among cross-border migrants in Thailand through systems thinking
Authors: Kunpeuk Watinee , Teekasap Pard , Kosiyaporn Hathairat , Julchoo Sataporn , Phaiyarom Mathudara , Sri Pigunkaew , Pudpong Nareerut , Suphanchaimat Rapeepong 
Published: March 2020

Abstract
Background The migrant health agenda in Thailand has been shaped by domestic and international pressures relating to social and economic dimensions. Thailand has become a popular destination for international migrant workers particularly from Cambodia, Lao PDR, and Myanmar. However, only a fraction of these migrant workers were insured by public health insurance. This study aimed to apply systems thinking to explore contextual factors affecting access to public health insurance arrangements among cross-border migrants in Thailand. Methods A group model building (GMB) was applied to explore contextual factors and their interplay influencing access to health insurance for migrants in Thailand. Participants (n = 20) were encouraged to share ideas about underlying drivers and barriers of access to health insurance among migrants. Triangulation was performed with the document review. The findings were analysed by a thematic approach. The causal loop diagramme (CLD) and stock and flow diagramme (SFD) were synthesised to identify the dynamics relating to access to migrant health insurance. Results Nationality verification was recognised as one of the most important mechanisms that the Thai government exercises to deal with the precarious citizenship status of undocumented migrants. Also, it is the key function that links economic necessity, national security, and public health concerns. In the formal sector, the government plays an important role in the enrollment of migrant workers in the Social Security Scheme (SSS) run by the Ministry of Labour (MOL). In the informal sector, the Health Insurance Card Scheme (HICS) managed by the Ministry of Public Health (MOPH) is the key option for migrant workers. However, some migrants are still left uninsured. The likely explanations are the semi-voluntary nature of the HICS, administrative delay of the enrollment process, and the resistance of some employers to hiring migrants. Conclusions Effective communication is required to raise awareness and acceptance towards the insurance among migrants and their employers. This needs cohesive effort from all stakeholders in society including the government, the business sector, civic groups, and even migrant communities. A public policy participatory process is needed to create a good balance of migrant policies among diverse authorities.