bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development and Delivery of Enhanced Implementation Support to Disseminate a National Caregiver Skills Training Program
Authors: Hughes Jaime M , Makaroun Lena K , Decosimo Kasey , Tucker Matthew , Dadolf Joshua , Drake Connor , Zullig Leah L , Coffman Cynthia J , Kota Swetha , Sperber Nina R , Christensen Leah , Chadduck Trisha , Allen Kelli D , Hastings Susan Nicole , Van Houtven Courtney H 
Published: December 2024

Abstract

Background and Objectives
As the population ages there is an increasing need for caregiver training programs, but little is known about how to deliver implementation support for diverse sites in large-scale implementation efforts. External group-based implementation facilitation may be one promising approach. This study’s objective is to detail the development and delivery of a pragmatic implementation facilitation approach to support the national rollout of caregiver training, Caregivers FIRST, at over 140 Veterans Health Administration (VHA) sites.

Research Design and Methods
Using administrative, survey, and project data, we describe the process of developing and delivering enhanced support to 13 VHA sites unable to meet adoption benchmarks as part of a national mandate. Enhanced support consisted of 4 group-based calls delivered within a 12-week period that used tailored external facilitation to address barriers, facilitators, and strategies for program implementation. We present key implementation barriers, implementation strategies, and sites’ perceptions of highly valued elements of enhanced support.

Results
Enhanced support calls focused on 3 implementation barriers commonly identified by implementing staff (n = 25) in a site-level needs assessment survey: recruiting and retaining caregivers (83%), balancing program delivery alongside competing demands (57%), and support from other departments in program delivery (44%). Sites attended a median of 3 out of 4 enhanced support calls. In postimplementation quantitative surveys, the average response of perceived helpfulness of enhanced support (ranging from 1 to 5) was 3.4 at 6 months, increasing to 4.2 at 12 months. Respondents valued collaborative problem-solving and the ability to learn best practices from other implementing sites during enhanced support calls.

Discussion and Implications
Describing the process of developing and delivering enhanced support via group-based external facilitation for Caregivers FIRST in VHA may provide useful information to guide similar efforts in other healthcare systems as they broadly disseminate interventions to support caregivers across diverse sites.

Clinical Trial Registration
NCT05319535
Building a holistic health approach in supported housing for people with intellectual disabilities in Denmark
Authors: Flensborg Jensen Maya Christiane , Rasmussen Pernille Skovbo , Olsen Leif , Røgeskov Maria , Ladekjær Else 
Published: December 2024

Abstract
People with intellectual disabilities (IDs) face health issues and barriers to physical activity. Health promotion programmes targeting this group are often short-term. Few programmes have been designed for people with IDs who live in supported housing staffed by social care workers (SCWs). The potential that SCWs hold as health promoters has recently come into focus within literature with a setting approach. Drawing on an ethnographic study, this article explores how SCWs articulate health promotion and enact health promoter roles at supported housing for adults with moderate to severe IDs. Our findings show that SCWs perceive sport and diet as health promotion activities. Because of their background within social education and not health promotion, few perceived themselves as health promoters. However, using the holistic concept of active living, which focuses on everyday lifestyle rather than short-term programmes, our ethnographic data reveal the more unspoken and unconscious practices that SCWs enact to ‘build’ customized active living routines among residents. Our results suggest that this ‘silenced’ capacity of SCWs to build healthy routines adds to previous findings by emphasizing that, rather than lacking a health promotion ethos, employees may have the potential to promote health by adapting a more holistic approach to health promotion. We argue the active living approach can contribute to building such a holistic health approach. Such an approach may help SCWs identify with and strengthen their enactment of health promotion roles.
Molecular phylogeny of the large-bodied dendryphantines of North America: Paraphidippus, Phidippus, Pelegrina, and their relatives (Salticidae: Dendryphantina)
Authors: Guerrero-Fuentes Dariana R , Francke-Ballvé Oscar F , Ceccarelli Fadia Sara 
Published: December 2024

Abstract
Despite the great advances in salticid systematics, the phylogeny of the subtribe Dendryphantina remains in part unresolved and many of the taxonomic boundaries of the genera are still problematic since previous systematic phylogenetic studies relied on sparse taxonomic samplings. Thus, further in-depth studies of the different groups are required to establish the generic boundaries and to piece together the genus-level relationships. Here, we examine more closely the phylogeny of the large-bodied North American jumping spiders and their relatives: Paraphidippus, Pelegrina, Phidippus, Beata sensu stricto, Metaphidippus manni group species, Dendryphantes nigromaculatus, Eris militaris, and Nagaina incunda. We elucidate the relationships among the genera and test their monophyly, employing molecular data from the mitochondrial gene fragment cytochrome oxidase subunit I, and nuclear gene fragments from histone 3 and actin 5C, analyzed under maximum likelihood approaches. Based on our results, the North American species D. nigromaculatus is related to the Palearctic species of Dendryphantes. Meanwhile, the previously established relationship between the genera Phidippus, Paraphidippus, and Beata sensu stricto is confirmed, whereas Pelegrina, Eris militaris, Nagaina cf. incunda, and species from the Metaphidippus manni group form another clade. Within this clade, Pelegrina is nonmonophyletic and is closely related to the Metaphidippus manni group species, which indeed form a monophyletic group. Therefore, this study is crucial for singling out the taxa in need of revision, as well as consolidating important relationships within the subtribe Dendryphantina.
Repurposing zidovudine and 5-fluoro-2′-deoxyuridine as antibiotic drugs made possible by synergy with both trimethoprim and the mitochondrial toxicity–reducing agent uridine
Authors: Levenfors Jolanta J , Bjerketorp Joakim , Guss Bengt , Nord Christina , Cao Sha , Hughes Diarmaid , Broberg Anders , Öberg Bo 
Published: December 2024

Abstract

Objectives
The increasing frequency of antibiotic-resistant bacterial infections is a major public health challenge, and new antibiotic drugs are urgently needed. A rapid solution to the problem is to repurpose clinically approved compounds with antibacterial properties, such as the nucleoside analogues zidovudine (azidothymidine) or 5-fluoro-2′-deoxyuridine. Here we report the in vitro and in vivo antibacterial properties of double and triple combinations of azidothymidine or 5-fluoro-2′-deoxyuridine with uridine and/or trimethoprim.

Methods
We determined MICs of azidothymidine and 5-fluoro-2′-deoxyuridine, alone or combined with uridine and/or trimethoprim, against a selection of Gram-negative and Gram-positive bacteria. We also measured MICs of a selection of antibiotics of different classes as a function of uridine concentration. The efficacy of azidothymidine and 5-fluoro-2′-deoxyuridine with uridine and/or trimethoprim was measured in a murine peritonitis infection model.

Results
The addition of uridine enhanced the in vitro antibacterial activity of azidothymidine and 5-fluoro-2′-deoxyuridine, against Gram-negative and Gram-positive bacteria, respectively. Uridine also enhanced the in vitro antibacterial activity of azidothymidine/trimethoprim and 5-fluoro-2′-deoxyuridine/trimethoprim combinations. Triple combinations containing azidothymidine, trimethoprim and uridine, showed antibacterial synergy against Gram-negative bacteria (Escherichia coli and Klebsiella pneumoniae) whereas the 5-fluoro-2′-deoxyuridine, trimethoprim and uridine combination showed synergy against the Gram-positive Staphylococcus aureus. The positive effect of uridine on the efficacy of azidothymidine/trimethoprim combination was also observed in vivo in a murine E. coli peritonitis model.

Conclusions
Triple combinations of these clinically approved compounds warrant further investigations as therapies to combat antibiotic-resistant infections.
Treatment success prediction in patients with methicillin-resistant coagulase-negative staphylococci infections, using vancomycin AUC24/MIC ratio: a multicentre retrospective cohort study
Authors: Hanai Yuki , Matsumoto Kazuaki , Endo Aiju , Hanawa Kazumi , Hashi Hideki , Miyazaki Taito , Yamaguchi Tetsuo , Harada Sohei , Yokoo Takuya , Uekusa Shusuke , Asakawa Daiki , Yokoyama Yoshiaki , Maruyama Riku , Tsujimura Shun , Namiki Takaya , Isoda Ryo , Enoki Yuki , Taguchi Kazuaki , Matsuo Kazuhiro 
Published: December 2024

Abstract

Background
Although vancomycin is commonly used to treat methicillin-resistant coagulase-negative staphylococci (MRCoNS) infections, there are no clear guidelines for the optimal 24 h AUC24/MIC ratio. This study aimed to determine the target AUC24/MIC ratio associated with vancomycin-treated MRCoNS infection outcomes.

Methods
This multicentre retrospective cohort study included adult patients who received vancomycin for ≥5 days for bloodstream infections caused by MRCoNS between January 2018 and December 2023. Primary outcome was treatment success, defined as a composite of survival beyond 30 days, clinical success and microbiological eradication. Secondary outcomes included 30-day mortality, clinical success, microbiological eradication and nephrotoxicity. Receiver operating characteristic (ROC) curve analysis was used to identify the AUC24/MIC cut-off for treatment success. Multivariate regression analysis was used to determine the association between AUC24/MIC and outcomes.

Results
This study included 147 patients. ROC analysis identified a target AUC24/MIC ≥373 for treatment success. The overall treatment success rate (70.1%) was significantly higher in the above-average AUC24/MIC cut-off group (83.1%) than that in the below AUC24/MIC cut-off group (57.9%). Multivariate analysis confirmed that AUC24/MIC ≥373 was an independent predictor (adjusted OR = 10.227; 95% CI = 3.585–29.171). The 30-day mortality and microbiological eradication rates differed significantly between the below- and above-cut-off groups, whereas nephrotoxicity rates were comparable among the groups.

Conclusions
In treating MRCoNS infections, vancomycin AUC24/MIC ratio ≥373 was independently associated with favourable treatment outcomes. However, further prospective studies are required to confirm this target owing to the retrospective nature of this study.
Effects of dichloromethane, nitrate, and sulfhemoglobin-induced substances on carboxyhemoglobin detection: a comprehensive review
Authors: Wu Jialin , Luan Yujing , Zhang Qingxia , Wang Fanglin , Rao Yulan 
Published: December 2024

Abstract
Carbon monoxide (CO) is a common gaseous toxin that causes severe poisoning symptoms. Accurate detection of the formation of carboxyhemoglobin (COHb) in the blood is very important for the identification of CO poisoning. In this review, the effects of exogenous toxins, including dichloromethane (DCM), nitrite, and hydrogen sulfide, on the determination of COHb by spectrophotometry are summarized by comparing epidemiological data, case studies, and analytical methods. The mechanism of the effects of these exogenous poisons on COHb detection is described, and the extent of their influence on the clinical diagnosis and forensic identification of CO poisoning is discussed. We suggest that emergency medicine and forensic science practices need to improve the understanding of these toxins and optimize clinical diagnosis and evaluation strategies to address the effects of toxins on the determination of COHb.
The Place of Law in Technology Transitions: A Case Study from Chemical Risk Assessment
Authors: Čavoški Aleksandra , Holden Laura , Lee Robert 
Published: December 2024

Abstract
Chemical exposure can produce devasting harm to human health and the environment. The EU regulation requires the testing of substances to ascertain whether they exhibit hazards such that appropriate risk management measures must be applied. Traditionally, toxicity testing has involved using animals as a surrogate for humans. Over the years, the EU has adopted a long-standing policy commitment to comply with the 3Rs principle to Replace, Reduce and Refine the use of animals for the purposes of scientific testing with the aim of full replacement. However, the progress away from mammalian use in hazard assessment is painfully slow, notwithstanding the development of a variety of new approach methodologies (NAMs) central to the transition away from animal testing. This study employs insights from the literature on technology transitions to explore the limited take-up of NAMs into regulatory science. It argues that the development of NAMs is not a linear process and the change to NAMs is impacted not only by scientific barriers but more often by wider technical and social barriers to transition. Such barriers arise from different levels of interaction between various actors, aligning with the conceptual framework that is not technologically deterministic. This article focuses on legal and regulatory influences at the multi levels before suggesting that law may stifle rather than accommodate or facilitate beneficial and ethical technological development.
Fishbone perforation of the stomach into the lesser sac and pancreas with abscess, a fishy business
Authors: Arcia Alberto , Muñoz Gabriela Alexandra , Jimenez Galo E , Albuja Monica G , Yumbla Thais Suarez , Venegas Oswaldo H , Velasco Andrea G , Parrales Diana , Molina Gabriel A 
Published: December 2024

Abstract
Ingestion of foreign bodies is common in critical practice. Thankfully, most of these objects will pass without complications; however, sharp and metallic objects can cause severe complications like ulcers and perforation. Perforation of the gastrointestinal tract is rare; however, once it happens, prompt treatment is needed to prevent dreadful complications. We present the case of a 52-year-old patient who presented with abdominal pain and fever. A tomography detected a fishbone perforation of the stomach, which was in the lesser sac attached to the pancreas. After surgery, the patient successfully recovered.
Histidine limitation alters plant development and influences the TOR network
Authors: Guérin Amandine , Levasseur Caroline , Herger Aline , Renggli Dominik , Sotiropoulos Alexandros Georgios , Kadler Gabor , Hou Xiaoyu , Schaufelberger Myriam , Meyer Christian , Wicker Thomas , Bigler Laurent , Ringli Christoph 
Published: December 2024

Abstract
Plant growth depends on growth regulators, nutrient availability, and amino acid levels, all of which influence cell wall formation and cell expansion. Cell wall integrity and structures are surveyed and modified by a complex array of cell wall integrity sensors, including leucine-rich repeat (LRR)-extensins (LRXs) that bind RALF (rapid alkalinization factor) peptides with high affinity and help to compact cell walls. Expressing the Arabidopsis root hair-specific LRX1 without the extensin domain, which anchors the protein to the cell wall (LRX1ΔE14), has a negative effect on root hair development. The mechanism of this negative effect was investigated by a suppressor screen, which led to the identification of a sune (suppressor of dominant-negative LRX1ΔE14) mutant collection. The sune82 mutant was identified as an allele of HISN2, which encodes an enzyme essential for histidine biosynthesis. This mutation leads to reduced accumulation of histidine and an increase in several amino acids, which appears to have an effect on the TOR (target of rapamycin) network, a major controller of eukaryotic cell growth. It also represents an excellent tool to study the effects of reduced histidine levels on plant development, as it is a rare example of a viable partial loss-of-function allele in an essential biosynthetic pathway.
A case of Erdheim–Chester disease—a mimicker of IgG4-related disease and large vessel vasculitis
Authors: Miyagawa Ippei , Nakayamada Shingo , Nohara Hirotsugu , Kosaka Shumpei , Ueno Masanobu , Tanaka Yoshiya 
Published: December 2024

ABSTRACT
The patient was a 57-year-old man who developed bilateral thigh pain and chest tightness 1 year ago. Chest computed tomography (CT) scan showed reticular shadows, thickened interlobular septa in both lung fields, and pericardial effusion. Three months ago, his symptoms worsened. A contrast CT scan revealed increased pericardial effusion, multiple masses in the right atrium, soft tissue shadows suggestive of retroperitoneal fibrosis, and soft tissue shadows around the thoracic and abdominal aorta. He visited University Hospital of University of Occupational and Environmental Health, Japan suspecting IgG4-related disease (IgG4-RD) or large vessel vasculitis (LVV). Based on the involvement of various organs and bilateral thigh pain, Erdheim–Chester disease (ECD) was suspected, and an 18F-fluorodeoxyglucose Positron Emission Tomography (FDG-PET) scan was performed. In addition to increased accumulation around the right ventricle, right coronary artery, and aorta, increased accumulation was confirmed in the distal femurs and proximal tibias on both sides, strongly suggesting ECD. A bone biopsy confirmed the diagnosis of ECD, showing bone fibrosis with CD68-positive and CD1a-negative foam cell infiltration, which is a characteristic of ECD. ECD is an extremely rare form of non-Langerhans cell histiocytosis. ECD affects a wide variety of organs, and its imaging findings can sometimes resemble those of IgG4-related disease or LVV. However, bone lesions are characteristic of ECD and are a key finding for its diagnosis. When systemic organ lesions, including bone lesions, are present, ECD should be included in the differential diagnosis, and PET-CT should be considered.
A Behavioral Analysis of Factors That Influence Antibiotic Prescribing in Hospitals: A Metasynthesis of Reviews
Authors: Mabaya Gracia , Evans Jenna M , Longo Christopher J , Morris Andrew M 
Published: December 2024

Abstract
Antibiotic resistance is a global public health threat driven, in part, by antibiotic overprescription. Behavior change theories are increasingly used to try to modify prescriber behavior. A metasynthesis of 8 reviews was conducted to identify factors influencing antibiotic prescribing for adults in hospital settings and to analyze these factors using 4 behavior change frameworks. Forty-three factors were identified across 7 thematic categories and then mapped to the theoretical domains framework and capability-opportunity-motivation model of behavior. The behavior change wheel and behavior change techniques taxonomy were then used to identify appropriate interventions and their components. The domain “environmental context and resources” was coded the most often, followed by “social influences” and “beliefs about consequences,” revealing that prominent sources of antibiotic prescribing behavior are “physical opportunity” and “social opportunity.” Based on these results, suggested interventions include environmental prompts/cues, education on consequences of antibiotic overuse, social comparison and support, and incentives.
The physiological and molecular responses of potato tuberization to projected future elevated temperatures
Authors: Guillemette Abigail M , Hernández Casanova Guillian , Hamilton John P , Pokorná Eva , Dobrev Petre I , Motyka Václav , Rashotte Aaron M , Leisner Courtney P 
Published: December 2024

Abstract
Potato (Solanum tuberosum L.) is one of the most important food crops globally and is especially vulnerable to heat stress. However, substantial knowledge gaps remain in our understanding of the developmental mechanisms associated with tuber responses to heat stress. This study used whole-plant physiology, transcriptomics, and phytohormone profiling to elucidate how heat stress affects potato tuber development. When plants were grown in projected future elevated temperature conditions, abscisic acid (ABA) levels decreased in leaf and tuber tissues, whereas rates of leaf carbon assimilation and stomatal conductance were not significantly affected compared to those plants grown in historical temperature conditions. While plants grown in projected future elevated temperature conditions initiated more tubers per plant on average, there was a 66% decrease in mature tubers at the final harvest compared to those plants grown in historical temperature conditions. We hypothesize that reduced tuber yields at elevated temperatures are not due to reduced tuber initiation, but due to impaired tuber filling. Transcriptomic analysis detected significant changes in the expression of genes related to ABA response, heat stress, and starch biosynthesis. The tuberization repressor genes SELF-PRUNING 5G (StSP5G) and CONSTANS-LIKE1 (StCOL1) were differentially expressed in tubers grown in elevated temperatures. Two additional known tuberization genes, IDENTITY OF TUBER 1 (StIT1) and TIMING OF CAB EXPRESSION 1 (StTOC1), displayed distinct expression patterns under elevated temperatures compared to historical temperature conditions but were not differentially expressed. This work highlights potential gene targets and key developmental stages associated with tuberization to develop potatoes with greater heat tolerance.
Applying an evolutionary perspective to assisted reproductive technologies
Authors: Evans Jonathan P , Garcia-Gonzalez Francisco 
Published: December 2024

Abstract
Assisted reproductive technologies (ART) are commonly used to address human infertility and to boost livestock production. During ART, procedures such as in vitro fertilization, artificial insemination, and intracytoplasmic sperm injection introduce gametes and embryos to unnatural and potentially stressful conditions that can influence offspring health, often via epigenetic effects. In this perspective we summarize these key risks of ART for embryonic and longer-term offspring fitness, emphasizing the need for experimental research on animal models to determine causal links between ART and offspring fitness across multiple generations. We also highlight how ART can bypass a range of naturally and sexually selected mechanisms that occur in the female reproductive tract and/or via female secretions that ultimately determine which sperm fertilize their eggs. We further argue that this curtailment of female-modulated mechanisms of sperm selection may have important consequences for ART-conceived offspring. We encourage the development of ART methods that better mimic natural processes of sperm selection and embrace the fundamental principles of natural and sexual selection. Ultimately, the aim of this perspective is to encourage dialogue between the fields of evolutionary biology and applied areas of animal and human reproduction.