bluuebunny/crossref_metadata_2025_split huggingface dataset:
Depressive symptoms and health service utilization among Chinese middle aged and older adults: A national population-based longitudinal survey
Authors: Guo Jing , Kong Dexia , Zhang Anao , Fang Liming , Zhu Yingxue 
Published: May 2020

Abstract
Background: The prevalence of depressive symptoms has been steadily increasing in recent years, however middle aged and older adults was less likely to use mental health services. The aims of this study were to 1) examine the cross-sectional and longitudinal relationships between depressive symptoms and health services utilization among Chinese middle-aged and older adults; and 2) evaluate whether there exists a rural-urban difference in such relationships. Methods: Data was obtained from China Health and Retirement Longitudinal Study (CHARLS) in 2013 and 2015, a nationally representative survey of 13,551 adults aged 45 years and above in China. Multivariate logistic regression analyses were conducted to assess the cross-sectional and longitudinal relationship between depressive symptoms and health services utilization in the whole sample, and urban and rural subsamples respectively. Results: Depressive symptoms were positively associated with a greater likelihood of outpatient and inpatient health services utilization. In addition, the relationships between depressive symptoms and health service use were consistent across rural and urban settings, indicating the robustness of such findings across geographic areas. Conclusions: Findings indicate that depressive symptoms are significantly associated with both in-patient and out-patient health service utilization among Chinese adults. Screening for depressive symptoms needs to be incorporated in these care settings in China.
Nomogram for Prediction of the International Study Group of Liver Surgery (ISGLS) Grade B/C Posthepatectomy Liver Failure in HBV-Related Hepatocellular Carcinoma Patients: An External Validation and Prospective Application Study
Authors: Ye Jia-zhou , Mai Rong-yun , Guo Wei-xing , Wang Yan-yan , Ma Liang , Xiang Bang-de , Cheng Shu-qun , Li lequn 
Published: May 2020

Abstract
BackgroundTo develop a nomogram for predicting the International Study Group of Liver Surgery (ISGLS) grade B/C posthepatectomy liver failure (PHLF) in hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC) patients.MethodsPatients initially treated with hepatectomy were included. Univariate regression analysis and stochastic forest algorithm were applied to extract the core indicators and reduce redundancy bias. The nomogram was then constructed by using multivariate logistic regression, and validated in internal and external cohorts, and a prospective clinical application.ResultsThere were 900, 300 and 387 participants in training, internal and external validation cohorts, with the morbidity of grade B/C PHLF were 13.5%, 11.6% and 20.2%, respectively. The nomogram was generated by integrating preoperative total bilirubin, platelet count, prealbumin, aspartate aminotransferase, prothrombin time and standard future liver remnant volume, then achieved good prediction performance in training (AUC = 0.868, 95%CI = 0.808–0.880), internal validation (AUC = 0.868, 95%CI = 0.794–0.916) and external validation cohorts (AUC = 0.820, 95%CI = 0.756–0.861), with well-fitted calibration curves. Negative predictive values were significantly higher than positive predictive values in training cohort (97.6% vs. 33.0%), internal validation cohort (97.4% vs. 25.9%) and external validation cohort (94.3% vs. 41.1%), respectively. Patients who had a nomogram score < 169 or ≧ 169 were considered to have low or high risk of grade B/C PHLF. Prospective application of the nomogram accurately predicted grade B/C PHLF in clinical practise.ConclusionsThe nomogram has a good performance in predicting ISGLS grade B/C PHLF in HBV-related HCC patients and determining appropriate candidates for hepatectomy.
Effects of active gases on droplet transfer and weld morphology in pulsed-current NG-GMAW of mild steel
Authors: Liu Guoqiang , Tang Xinhua , Xu Qi , Lu Fenggui , Cui Haichao 
Published: May 2020

Abstract
Small amount of active gases CO 2 and O 2 were added into pure argon inert shielding gas to improve the weld formation of pulsed-current narrow-gap gas metal arc welding (NG-GMAW) of mild steel. Their effects on droplet transfer and arc behavior were investigated. A high-speed visual sensing system was utilized to observe the metal transfer process and arc morphology. When the proportion of CO 2 , being added into the pure argon shielding gas, changes from 5% to 5%, the metal transfer mode changes from pulsed spray streaming transfer to pulsed projected spray transfer, while it remains the pulsed spray streaming transfer when 2% to 10% O 2 is added. Both CO 2 and O 2 are favorable to stabilizing arc and welding process. O 2 is even more effective than CO 2 . However, O 2 is more likely to cause the inclusion defects in the weld, while CO 2 can improve the weld appearance in some sense. The weld surface concavity, which is sensitive to the formation of lack-of-fusion defect in NG-GMAW, is greatly influenced by the addition of active gas, but the weld width and weld penetration almost keep constant.
Evaluation and validation of technical procedure for foundry coke mechanical strength testing
Authors: Mertas Bartosz , Sobolewski Aleksander , Rozycki Grzegorz 
Published: May 2020

Abstract
Foundry coke is a special type of coke with lumps size usually bigger than 100 mm. Therefore it is difficult to asses its physical properties representatively for whole lot. There exist international standards for coke sampling and testing strength index (usually Micum test). Nevertheless companies dealing with foundry coke notified problems with methodology of sampling and sample preparation for the test. Manual sampling according to ISO 18283 is very labour extensive and with connection with literally proceeding with ISO 566 gives non-representative results for foundry coke +100 mm. For this reason on the basis of these standards there was evaluated procedure for coke sampling and testing strength indexes which is more practical for plant routine use and gives more reliable results in comparison to international standard. This article presents results of work aimed at checking usability of ISO standards provisions for foundry coke testing and evaluation and validation of simplified procedure.
Post-anesthetic equine myelopathy: characterization of neuronal reaction and axonal injury using beta-amyloid precursor protein immunohistochemistry
Authors: Fortin Jessica S , Kramer Joanne , Bukoski Alex D , Kim Dae Y 
Published: May 2020

Abstract
A 2-year-old female Clydesdale horse was anesthetized in dorsal recumbency for an elective surgical procedure to treat osteochondritis dissecans involving the intermediate ridge of the right tibia. Following surgery, the horse lost motor and sensory function in the hind limbs and was unable to stand. The status deteriorated and the horse was euthanized 20 h post-surgery. Histopathologic examination revealed mild to moderate acute myelopathy of the spinal cord between T14 and S3. The lesions were bilateral, with one side being slightly more affected, and more severe in the distal lumbar and sacral spinal cord segments. Immunohistochemistry for beta-amyloid precursor protein (β-APP) was compared to other fast axonal transport proteins such as neurofilament heavy protein, synaptophysin, and ubiquitin. In the presented case, the β-APP immunohistochemistry revealed positively-labeled, multiple, segmentally-swollen axons (axonal bulbs) and rarely neurons. The positively-stained areas overlapped with the lesions seen on HE stained slides including areas in which changes were poorly discernible by routine histologic exam. Amongst the fast axonal transport proteins detected via immunohistochemistry, β-APP was considered adequate to use as a potential sensitive biomarker for axonal injury in this case of post-anesthetic myelopathy. β-APP immunohistochemistry may be a useful tool to study and diagnose axonal injuries of the central and peripheral nervous system in veterinary medicine.
A new Syber Green real time PCR to detect SARS-CoV-2
Authors: Marinowic D.R. , Zanirati G. , Rodrigues F.V.F. , Grahl M.V.C. , Alcará A.M. , Machado D.C. , Costa J.C. Da 
Published: May 2020

Abstract
Phylogenetic analyses demonstrated that etiologic agent of pandemic outbreak is a betacoronavirus named SARS-CoV-2. For public health interventions, a diagnostic test with high sensitivity and specificity is required. The gold standard protocol for diagnosis by WHO is the RT-PCR. To detect low viral load and large-scale screening a low-cost diagnostic test becomes necessary. Here we develop a cost-effective test capable of to detect the new coronavirues. We validated an auxiliary protocol for molecular diagnosis with RT-PCR SYBR Green methodology to successfully screen negative cases of SARS-CoV-2. Our results demonstrated that a set of primers with high specificity, and no homology with other viruses from Coronovideae family or human respiratory tract pathogenic viruses. Optimization of annealing temperature and polymerization time led to an high specificity in the PCR products. We have developed a more affordable and swift methodology for negative SARS-CoV-2 screening. This methodology can be applied on large scale populational to soften panic and economic burden through guidance for isolation strategies.
Covid-19 mortality is associated with test number and government effectiveness
Authors: Liang Li-Lin , Tseng Ching-Hung , Ho Hsiu J. , Wu Chun-Ying 
Published: May 2020

Abstract
Objectives: A question central to the Covid-19 pandemic is why the Covid-19 mortality rate varies so greatly across countries. This study aims to investigate factors associated with cross-country variation in Covid-19 mortality.Methods: Covid-19 mortality rate was calculated as number of deaths per 100 Covid-19 cases. To identify factors associated with Covid-19 mortality rate, a multivariable linear regression model was applied to a cross-sectional dataset comprising 78 countries and 1,790,550 patients infected by Covid-19. We retrieved data from the Worldometer website and the Worldwide Governance Indicators and World Development Indicators databases.Results: Covid-19 mortality rate was negatively associated with Covid-19 test number per 1,000 population (RR=0.97; 95% CI 0.96 to 0.99, P=0.013) and government effectiveness indicator (RR=0.96; 95% CI 0.93 to 0.98, P=0.001). Covid-19 mortality rate was positively associated with number of critical cases per 100 Covid-19 cases, Covid-19 case number per 10,000 population, proportion of population aged 65 or older and proportion of deaths attributable to communicable diseases in previous years (all with P<0.05). Predicted mortality rates were highly associated with observed mortality rates (r = 0.74; P<0.001).Conclusions: Multiple factors were associated with Covid-19 mortality rates. Increasing Covid-19 testing and improving government effectiveness may have the potential to attenuate Covid-19 mortality.Authors Li-Lin Liang and Chun-Ying Wu contributed equally to this work.
Desempeño de una Oficina de Transferencia de Tecnología en el contexto de gestión de patentes: Estudio de caso de la OTT de la Benemérita Universidad Autónoma de Puebla
Authors: Lima Antonio Solís , Ovando Catalina , Olivera Pérez Emmanuel , Rodríguez Lozada Miguel Ángel 
Published: May 2020

Introducción: Con la finalidad de detonar la transferencia de conocimiento, el gobierno mexicano, ha establecido la política pública de la creación de Oficinas de Transferencia de Tecnología (OTT); sin embargo, esta política ha mostrado ser ineficaz debido a la inconsistencia en la permanencia de la mayoría de las OTT mexicanas. A pesar de esto, existen algunas OTT que han sobresalido; tal es el caso de la OTT de la Benemérita Universidad Autónoma de Puebla (OTT-BUAP) que fue establecida en el 2011. Dicha oficina ha posicionado a esta universidad en los primeros lugares a nivel nacional en el contexto de generación de patentes, razón para que esta OTT haya sido el objeto de análisis de este estudio para dar respuesta a la siguiente pregunta de esta investigación: ¿Cómo ha logrado la OTT-BUAP posicionarse dentro de las mejores OTT en México, en el ámbito de gestión de patentes, durante el periodo 2011-2018?El objetivo general de esta investigación es contribuir al conocimiento del especialista en transferencia y comercialización de tecnología que se dedica a la gestión de las OTT Universitarias. En cuanto a la revisión de la literatura, se encontró que este tema relacionado con el desempeño de las OTT mexicanas en el ámbito de gestión de patentes ha sido poco estudiado, por lo cual se justifica esta investigación.Método: Esta investigación se considera de tipo exploratorio, cualitativo, descriptivo y transversal, la cual consistió en aplicar la metodología del estudio del caso a la OTT-BUAP, basándose en seis proposiciones formuladas a partir de fuentes secundarias.Resultados: Se halló que de los procesos de protección de propiedad intelectual, consultoría, licenciamiento y la creación de spinoffs, solo el proceso de protección de propiedad intelectual es el que se encuentra en etapa de maduración en dicha OTT. También, se halló que la OTT-BUAP cuenta con un equipo experto en la redacción de patentes, el cual fue contratado específicamente, para la creación e impulso de dicha OTT.Discusión o Conclusión: Según los resultados encontrados en esta investigación, la razón por la que la OTT-BUAP se ha posicionado dentro de las mejores OTT en México en el periodo 2011-2018, se debe, en gran medida, a que cuenta con un proceso de patentamiento eficaz, basado en una normativa equitativa en la repartición de regalías apoyado en un plan estratégico de incentivos para motivar a los investigadores a proteger los resultados de sus investigaciones. Dicho proceso fue diseñado y gestionado por un investigador proveniente del Instituto Mexicano de la Propiedad Industrial, experto en protección de la propiedad intelectual, contratado por la BUAP, específicamente, para conformar un equipo de trabajo profesional para la redacción de patentes y otras tareas relacionadas con la transferencia y comercialización de la tecnología proveniente de la BUAP.
Conduction Disorders: The Value of Surface ECG
Authors: da Silva Rose M.F.L. , de Souza Maciel Alessandra 
Published: May 2020

Purpose of review:
The purpose of the current mini-review is to describe the
importance of surface ECG for the diagnosis of conduction disorder.

Methods:
The MEDLINE/PubMed database was used, with the keywords "ECG" and "conduction
disorders"; over the past 10 years. Other documents were included because of their relevance.

Main findings:
Data on the anatomy and function of the cardiac electrical system have been
described. Conduction disorders including sinus node dysfunction, atrioventricular blocks, intraventricular
conduction disorders are exposed as to their epidemiology, etiology, presentation, anatomical
site of impaired conduction of the electrical stimulus. The importance of ECG in patients
with a cardiac implantable electronic device was also discussed, in addition to future perspectives.

Conclusion:
Surface ECG allows the diagnosis of atrioventricular and intraventricular conduction
disorder and its anatomical block site most of the time, without the need for invasive tests such as
electrophysiological study. Dysfunctions of cardiac implantable electronic devices can be diagnosed
by ECG, as well as the prediction of response to cardiac resynchronization therapy.
The Crystal Structures of Thermomyces (Humicola) Lanuginosa Lipase in Complex with Enzymatic Reactants
Authors: McPherson Alexander , Larson Steven B. , Kalasky Andrew 
Published: May 2020

Aim:
To understand the details of the action of fungal lipase and the mechanism for its observed
interfacial activation.

Background:
Fungal lipase, crucial to biotechnology, functions at the lipid - water interface where it
undergoes a poorly understood interfacial activation. Biochemical factors influencing its activation
and inhibition are also poorly understood. This study provides a basis for its activity and a plausible
mechanism for interfacial activation.

Objective:
To determine the structures of fungal lipase in different crystal forms in complex with
their enzymatic reactants and inhibitors.

Method:
X-ray crystallography.

Results:
Thermomyces lanuginosa lipase was visualized in three crystal forms, of space groups H32,
P21 and I222 at 1.3 to 1.45 Å resolution. Rhombohedral crystals have one molecule, lacking segment
241 to 252, as an asymmetric unit, with molecules organized as two trimers. Monoclinic crystals’
asymmetric unit is six intact molecules organized as two, nearly identical trimers, each exhibiting an
NCS threefold axis. The “lid” helix was consistently closed. Oligomerization into trimers creates an
internal hydrophobic cavity where catalysis occurs. In monoclinic and orthorhombic crystals, active
site serines were esterified to fatty acids. Lipase had bound within their trimeric, hydrophobic cavities
1,3-diacylglycerols with fatty acid chain lengths of about 18 carbons.

Conclusions:
Results suggest trimers are likely the active form of the enzyme at the lipid-water interface.
Formation of trimers may provide an explanation for “interfacial activation”.
Enhanced Ofloxacin Degradation Efficiency on Porous CeTi2O6 Photocatalyst - CTAB Induced Porosity
Authors: Yang Lili , Li Chuanguo , Zhang Wenjie 
Published: May 2020

Background:
Photocatalytic oxidation of organic pollutants in the environment is being studied
for more than half a century. Titanate has the activity on the degradation of organic pollutants under UV light
illumination. Template directed sol-gel method is capable of producing porous structure in titanate during
high temperature thermal treatment.

Methods:
The materials were characterized using X-ray powder diffraction, transmission electron microscopy,
scanning electron microscopy, surface area and pore size analyses, UV-Visible spectrometry, and Xray
photoelectron spectroscopy. Photocatalytic activity of the CeTi2O6 material was evaluated through ofloxacin
degradation.

Results:
Brannerite structured CeTi2O6 was the major component in the samples, and the addition of CTAB
caused a slight growth of CeTi2O6 crystals. Porous structure formed in the porous sample after the removal
of CTAB template, and the surface area and pore volume were greatly enlarged. The first order reaction rate
constant for photocatalytic degradation of ofloxacin was 9.60×10-3 min-1 on the nonporous CeTi2O6 sample,
and it was as large as 2.44×10-2 min-1 on the porous CeTi2O6 sample. The addition of CTAB can influence
the physico-chemical properties of the porous CeTi2O6, such as the improved activity on photocatalytic degradation
of ofloxacin.

Conclusion:
The CeTi2O6 samples composed of majority brannerite CeTi2O6, and CeTi2O6 crystallite
sizes for the nonporous and porous samples were 38.1 and 43.2 nm. The burning up of CTAB
during calcination produced abundant pores in the porous material. After 50 min of reaction, photocatalytic
degradation efficiencies on the nonporous and porous CeTi2O6 samples were 38.1% and
70.5%.
Integrative Analysis of miRNA-mediated Competing Endogenous RNA Network Reveals the lncRNAs-mRNAs Interaction in Glioblastoma Stem Cell Differentiation
Authors: Zhao Zhenyu , Zhang Cheng , Li Mi , Yu Xinguang , Liu Hailong , Chen Qi , Wang Jian , Shen Shaopin , Jiang Jingjing 
Published: May 2020

Background:
Competing endogenous RNA (ceRNA) networks play a pivotal role in
tumor diagnosis and progression. Numerous studies have explored the functional landscape and
prognostic significance of ceRNA interaction within differentiated tumor cells.

Objective:
We propose a new perspective by exploring ceRNA networks in the process of
glioblastoma stem cell (GSC) differentiation.

Methods:
In this study, expression profiles of lncRNAs and mRNAs were compared between GSCs
and differentiated glioblastoma cells. Using a comprehensive computational method, miRNAmediated
and GSC differentiation-associated ceRNA crosstalk between lncRNAs and mRNAs was
identified. A ceRNA network was then established to select potential candidates that regulate GSC
differentiation.

Results:
Based on the specific ceRNA network related to GSC differentiation, we identified lnc
MYOSLID: 11 as a ceRNA that regulated the expression of the downstream gene PXN by
competitively binding with hsa-miR-149-3p. After Kaplan-Meier (KM) survival analysis, the
expression of PXN gene (PPXN = 0.0015) and lnc MYOSLID: 11 (PMYOSLID: 11=0.041) showed
significant correlation with glioblastoma in 160 patients from TCGA.

Conclusion:
This result sheds light on a potential way of studying the ceRNA network, which can
provide clues for developing new diagnostic methods and finding therapeutic targets for clinical
treatment of glioblastoma.
Possible Protective Effects of Curcumin via Modulating of Androgen Receptor (AR) and Oct2 Gene Alterations in Cisplatin-Induced Testicular Toxicity in Rat
Authors: Fard Amin A. , Samadi Mahrokh , Biabangard Abdolrahman 
Published: May 2020

Background:
Cisplatin is a chemotherapeutic drug used to treat testicular cancer that induces
testicular toxicity. This study aimed to investigate the possible role of androgens, androgen receptor,
and organic cation transporter 2 (OCT2) in the protective effects of curcumin on cisplatininduced
testicular toxicity.

Methods:
Thirty male Wistar rats were divided into five groups: 1- control (normal saline, 0.5 ml ip,
daily for 10 consecutive days); 2- cisplatin (10 mg/kg ip, single dose at the first day); 3- cisplatin +
curcumin (10 mg/kg ip, dissolved in 5% DMSO, daily for 10 consecutive days); 4- cisplatin + vehicle
(DMSO 5%, 0.3 ml ip); and 5- curcumin (10 mg/kg ip). At the end of the study, a blood sample was
obtained for testosterone measurement. The left testis was kept at -80 to measure androgen receptor
(AR) and type 2 organic cation transporter (OCT2) gene expression and the right testis were kept in
10% formalin for histological analysis.

Results:
Cisplatin significantly decreased serum testosterone, declined testis AR gene expression, and
increased OCT2 gene expression in testis (p<0.01). Curcumin treatment significantly prevented these
alterations in testosterone and gene expressions (p<0.01). Moreover, curcumin significantly reversed
the cisplatin-induced kidney tissue injury and increased spermatid and spermatozoa.

Conclusion:
It is concluded that the ameliorative effect of curcumin in cisplatin-induced reproductive
disorders was due to the modulation of testosterone and androgen receptors.