bluuebunny/crossref_metadata_2025_split huggingface dataset:
AKAP95 transports Cx43 into nucleus, regulating G1/S transition of A549 cells.
Authors: Deng Zifeng , Liu Rong , Guo Huiling , Yao Luming , Ruan FengKai , Wang Kai , Qiu Guihua , Luo Zhen , Luo Guangping , Guo DongBei , Yao Youliang , Zhang Yongxing 
Published: October 2020

Abstract
Purpose: Cx43 (connexin 43) has been found to inhibit cell cycle of tumor cells. No nuclear localization sequence of Cx43 was found but the protein has been found in nucleus. The process remains unclear. We are aiming at providing the model that AKAP95 (A-kinase anchoring protein) binds to and transports Cx43 into nucleus of lung cancer cells.Methods: Lung cancer cells were arrested at preliminary stage (P), middle and late stage (M) of G1 phase and restriction point (R). Immunocoprecipitation (Co-IP) and Western blot (WB) were used to analyze expression and binding of AKAP95, Cx43, cyclin D1 and cyclin E1 at different stages of lung cancer cells during G1 phase. Confocal laser scanning microscopy (CLSM) was used to detect the location of proteins in arrested cells. Transmission electron microscope (TEM) with higher resolution was used to detect binding/aggregating and location of the above four proteins at subcellular level.Results: 1) AKAP95 transports Cx43 into the nucleus through the nuclear pore mainly at R stage. Some AKAP95 and Cx43 form protein aggregates during the process. 2) Cyclin E1 was found to be bind/aggregate with AKAP95-Cx43 complex and involved in the nuclear transport of Cx43 mainly at R stage. 3)Cyclin D1 was found to bind with AKAP95 and Cx43 respectively but not to be involved in their aggregating and nuclear transport of Cx43.Conclusions: 1) AKAP95 and Cx43 bind/aggregate to each other during G1 phase, forming AKAP95-Cx43 complexes/aggregates. After their binding/aggregating, AKAP95 targets nuclear pore and matrix, transporting Cx43 into nucleus. Formed AKAP95-Cx43 complexes/ aggregates do not dissociate in nucleus. The process can be summarized as ‘forming AKAP95-Cx43 complexes/aggregates; transporting Cx43 into nucleus; functioning remaining binding/aggregating’. 2) Cyclin D1 was only found to bind to AKAP95 and Cx43 respectively and function in cytoplasm while cyclin E1 was involved in the binding/aggregation of AKAP95 and Cx43, as well as the nuclear transport of Cx43.
Genetic variability in IGFBP-3 and GH genes and their association with body weight and growth performance at birth, weaning and six-month in sheep&nbsp;
Authors: Saleh Ahmed , Hammoud Mohammed , Dabour Nasraa , Hafez Elsayed , Sharaby Mahmoud 
Published: October 2020

Abstract
Body weights at birth, weaning and six-month and growth performance in lambs are economic traits that could be strongly used in sheep breeding objectives. Moreover, previous genetic studies recommended strong associations between these economic traits and polymorphisms in several candidate genes in different livestock species. The aim of this investigation was to study the polymorphism in IGFBP-3 and GH genes using DNA sequencing and RFLP, and their association with body weights and growth performance of Rahmani, Barki, Rahmani X Barki cross, Awassi/Awassi X Suffolk cross and Ossimi sheep breeds. Digestion of 654 bp for IGFBP-3/HaeIII yielded only one restriction pattern of 8 fragments in all animal groups revealing the absence of polymorphism also, DNA sequencing confirmed that. While, sequencing analysis of the ampliﬁed fragment of GH gene revealed polymorphism in nucleotide sequencing, and the amino acid sequences for GH gene of Rahmani were different from those of other breeds in 4 amino acids, while, each of Rahmani X Barki cross and Ossimi amino acid sequences were different in 3 amino acids. The genetic differences in GH gene coincided with differences in birth, weaning and six-month weights of the breeds under study. Thus, the current results suggest that body weights and growth performance of tested sheep breeds at different stages of growth are statistically affected by GH gene which could be considered as a candidate gene for growth in breeding programs.
Helicobacter pylori 23S rRNA gene A2142G, A2143G, T2182C, and C2195T mutations associated with clarithromycin resistance detected in Sudanese patients
Authors: Albasha Aalaa Mahgoub , Alnosh Maram M. , Osman Esraa Hassan , Zeinalabdin Duha M , Fadl Amira A M ,   Musa Abdalla Ali3, Altayb Hisham N. 
Published: October 2020

Abstract

Background: Clarithromycin resistant Helicobacter pylori (H. pylori) strains represent a worldwide health problem. These stains are usually carrying mutations within the 23S rRNA gene associated with clarithromycin resistance. This study aimed to detect H. pylori and clarithromycin resistant associated mutations from Sudanese patients with gastritis symptoms.Materials and Methods: Two hundred and eighty-eight gastric biopsies were collected using gastrointestinal endoscopy from patients with gastritis symptoms in different hospitals in Khartoum state. H. pylori was detected by PCR using primers targeting 16S rRNA and 23S rRNA. Then allele-specific PCR and DNA sequencing were used to screen for the presence of A2142G and A2143G point mutations.Results: Out of 288 samples, H. pylori was detected in 97 (33.7%) sample. Allele-specific PCR detected the variant A2142G in 9/97 (9.3%) sample, while A2143G mutation was not found in any sample. The DNA sequencing revealed the presence of mutations associated with clarithromycin-resistance in 48% (12/25) of samples; the A2142G was present in one sample, A2143G in 5 samples, T2182C in 4 samples, and C2195T in 3 samples. There was no association of 23S rRNA gene point mutations with gender, age group and geographical distribution of patients.Conclusion: This study revealed a high frequency (48%) of mutations associated with clarithromycin resistance using DNA sequencing of the 23S rRNA gene's V domain. This information should be taken into consideration before choosing optimal therapy for H. pylori eradication.
Unravelling a PhIL1-glideosome associated complex in Plasmodium falciparum merozoite that is essential for invasion in host erythrocytes
Authors: Saini Ekta , Sheokand Pradeep Kumar , Sharma Vaibhav , Agrawal Prakhar , Kaur Inderjeet , Singh Shailja , Mohmmed Asif , Malhotra Pawan 
Published: October 2020

Abstract
The human malaria parasite, Plasmodium falciparum possess a unique mechanism of gliding motility guided by glideosome that powers its entry into insect and vertebrate hosts to facilitate its invasion and internalization within the targeted host cell. Photosensitized INA-labelled protein 1 (PhIL1) forms a novel protein complex that is associated with glideosome motor complex in the inner membrane complex of invasive merozoite. To establish the role of PfPhIL1 associated novel complex at asexual blood stages, we characterized three proteins associated with PhIL1: a glideosome associated protein- PfGAPM2, an IMC structural protein- PfALV5 and a previously uncharacterised protein - referred here as PfPhIP (PhIL1 interacting protein). GFP targeting and co-immunoprecipitation analysis confirmed that these proteins are part of a PhIL1 associated novel complex, which co-exists with the glideosomal complex. To know the functional significance of PhIL1 associated complex, transgenic parasites were generated for glmS mediated conditional knock-down of each of the three proteins. Parasites lacking PfPhIP or PfGAPM2 were unable to invade the RBCs. PfPhIP deficient parasites also showed defects in merozoite segmentation. PfPhIP and PfGAPM2 depleted parasites revealed abrogation of reorientation/gliding, although initial attachment with human RBCs was not affected in these knock-down parasites. Together, the data presented here shows that proteins of the PhIL1 associated complex play an important role in orientation of P. falciparum merozoites post initial attachment, which is crucial for formation of tight junction and hence invasion of host erythrocytes.
The Relation Between Trace Element Status (Zinc, Copper, Magnesium) and Clinical Outcomes in COVID-19 Infection During Pregnancy
Authors: Anuk Ali Taner , Polat Naci , Akdas Sevginur , Erol Seyit Ahmet , Tanacan Atakan , Biriken Derya , Keskin Huseyin Levent , Tekin Ozlem Moraloglu , Yazihan Nuray , Sahin Dilek 
Published: October 2020

Abstract
Background: SARS CoV-2 is a novel coronavirus which cause recent pandemia and many deaths. This study aimed to determine zinc, copper and magnesium status on pregnant women with COVID-19.Methods: Totally 200 pregnants were included in the study, 100(34/33/33) were pregnants with COVID-19 infection and 100(33/32/35) were healthy pregnants and each group was divided into trimesters. Blood samples for trace element measurements were obtained from the patients along with the initial laboratory tests for clinical outcomes upon their first admission to hospital.Zinc, copper and magnesium levels were analyzed with atomic absorption spectroscopy.Results: In the first/third trimesters, serum zinc level was lower (p:0,004)/(p:0,02), serum copper level was higher (p:0,006)/(p:0,008), Zn/Cu ratio was decreased (p<0,0001)/(p<0,0001) and serum magnesium level was also higher (p<0,0001)/(p<0,0001) in COVID-19 group compared to control. Pregnants with COVID-19 in their second trimester had lower serum zinc level (p:0,05) and also copper level was decreased (p:0,0003) compared to control. Our correlation results showed that serum zinc and Zn/Cu ratio levels had negative relation with acute phase markers such as IL-6, Erythrocyte Sedimentation Rate, procalcitonin and Creactive Protein. Also, increased serum magnesium level might have role on decreased white blood cell, neutrophil, lymphocyte cell concentrations and there was correlation between serum magnesium and CRP level in the third trimester.Conclusion: This study indicated that trace element status changed in pregnants with COVID-19. With the further studies,it will be clarified that the effects of trace elements on COVID-19 in pregnant and in general population which was shown with this study first time to date.
Undernutrition and Associated Factors Among Lactating Women in Dire Dawa Health Facilities, Ethiopia
Authors: Tariku Zerihun , Tefera Bereket , Samuel Serawit , Derese Tariku , Markos Melese , Dessu Samuel 
Published: October 2020

Abstract
Background: The nutritional status of lactating women is very important since it also affects the health of their children. However, there was limited information on maternal nutrition status in low-income countries like Ethiopia, especially in the study area.Objectives: Determine magnitude of undernutrition and associated factors among lactating womenMethods: Institutional based cross-sectional study was conducted among 422 lactating women in Dire Dawa town health facilities from February 10/02/2019 – March 30/ 03/2019.Result: Prevalence of undernutrition was 22 %. Women who age 15-25 years were four times more likely undernourished than older [AOR=4.04(CI: (1.74, 9.40)]. Unable to read and write Women were almost five times more likely to be undernourished than formal education [AOR=4.76 CI: (2.31, 9.81)]. Women who have family size >7 were six times more likely to be undernourished than family size < 3 [AOR=5.53 CI :( 1.15, 26.53)]. Women not take additional food during lactating were 4.5 times more likely undernourished than take additional food [AOR=4.56 CI (1.50, 13.9)]. DD score < 5 were four times more likely to be undernourished than (>= 5) DD [AOR= 4 CI: (2.02, 7.90)].Conclusion: Prevalence of undernutrition in the study area was high: Factors associated with underweight were: Age of lactating women, Education status, Family size, Additional food during lactation and DD score. Thus, multi-sectoral collaboration targeted at improving women’s educational status and increasing food during lactation need to be emphasized.
Evaluating adenosine triphosphate bioluminescence for biomonitoring in potable water systems
Authors: Rodriguez Angela B. , Duranceau Steven J. 
Published: October 2020

Abstract
The application of adenosine triphosphate (ATP) bioluminescence technology as a rapid microbiological activity monitor within a potable water system has been demonstrated. ATP measurements were taken pre- and post-infrastructure improvements within a watershed and throughout two potable water systems from source to tap. A reduction in ATP, as measured by relative light units, was identified post-infrastructure improvements. Peak ATP values for the watershed were found within the reservoirs. The treated source water remained biologically stable throughout the distribution system, with peaks attributed to nitrification. A mathematical model for predicting microbial ATP using pH, temperature, and alkalinity, was developed for the watershed studied, with an adjusted R2 of 0.84 at a 95% confidence level. Overall, ATP bioluminescence technology was found to be a suitable candidate for rapid microbiological activity testing in drinking water systems; however, technological limitations remain with respect to reproducibility that should be addressed prior to full-scale implementation.
Use of group maturity index to measure growth, performance, and sustainability of community health clubs in urban water, sanitation and hygiene (WASH) program in Zimbabwe
Authors: Hirai Mitsuaki , Cole Arnold , Munyaka Moreblessing , Mudhuviwa Steven , Maja Taurai , Cronin Aidan 
Published: October 2020

Abstract
Zimbabwe experienced an unprecedented cholera outbreak in 2008 and 2009. Reduced access to water, sanitation and hygiene, delayed community health education, and limited knowledge on cholera prevention were the major risk factors of this outbreak which were addressed by urban WASH interventions. Health and hygiene promotion through community health clubs (CHCs) is a cost-effective strategy to reduce the risk of cholera. In 2013, UNICEF Zimbabwe launched the Small Towns WASH Program (STWP) and used the CHC approach for hygiene promotion. To monitor the growth, performance, and sustainability of CHCs, STWP employed the Group Maturity Index, which measures the status of CHCs in five domains: objectives, governance, resources, group systems, and impacts. This study described the maturity status of CHCs as measured by GMI as a new monitoring tool and assessed if CHCs’ performances in GMI's output domains are associated with the impact domain. The results suggested that over 75% of CHCs had reached the managed stage or the mature stage by 2018. Three of the GMI's output domains were independently associated with the overall impact domain after controlling for potential confounders. CHCs and club members may experience overall positive impacts by developing their governance, resource, and group system domains.
Bio-adsorbent preparation based on Chinese Radix isatidis residue for Pb(II) removal
Authors: Shi Yu-zhuo , Yin Xiao-chun , Si Guang-hui , Zhang Na-di , Du Mei-xia , Wang Xin-hua 
Published: October 2020

AbstractA series of bio-adsorbents with potential for Pb(II) removal from wastewater were prepared by treating Radix isatidis residue (RIR) with integrated chemical treatment and fermentation methods. Batch experiments were used to test the adsorption and desorption performance of different bio-adsorbents. The results showed that treated RIRs had significantly enhanced adsorption capacity of 23.5 and 27.6 mg g−1 for Pb(II) within 50 minutes, in contrast to the raw RIR's 12.2 mg g−1. RIR produced by modified chemical/fermentation treatment can remove up to 31.1 mg g−1. After five adsorption/desorption cycles, about 75% of the adsorption capacities were maintained. This study is a novel approach to reusing the enormous quantity of Chinese herbal medicine residues.
Research on high photosynthetic efficient cultivation with drip irrigation under different mulch of maize
Authors: Bai Yu , Gao Jinhua 
Published: October 2020

AbstractMaize refers to one of the major food crops worldwide. Its yield has a direct effect on global food security. Moreover, cultivated lands in the world have been undergoing serious degradation. In the present study, field experiments were performed in the middle of Jilin, China. A novel maize cultivating method, combining drip irrigation under film mulch cultivation and high photosynthetic efficient cultivation, is presented. NUE, WUE, accumulated temperature, plant growth and yield were determined in high photosynthetic efficient cultivation with drip irrigation under mulch and with there being under the mulch transparent and black film, respectively. As revealed from the results, the high photosynthetic efficient cultivation based on drip irrigation cultivation could increase the yield of rain-fed treatment by 53%; high photosynthetic efficient cultivation with drip irrigation under film mulch cultivation could also improve NUE and WUE compared with conventional drip irrigation under film mulch cultivation; transparent mulch could more significantly raise the soil temperature than black mulching, as well as improving the maize yield. The present study presents a novel planting mode in accordance with reliable theory to sustainably develop maize.
In-silico Molecular Interaction of Short Synthetic Lipopeptide/Importin-alpha and In-vitro Evaluation of Transgene Expression Mediated by Liposome- Based Gene Carrier
Authors: Tarwadi Tarwadi , Jazayeri Jalal A. , Pambudi Sabar , Arbianto Alfan D. , Rachmawati Heni , Kartasasmita Rahmana E. , Asyarie Sukmadjaja 
Published: October 2020

Background:
Lipopeptide-based gene carriers have shown low cytotoxicity, are capable
of cell membrane penetration, are easy to manufacture and therefore are great potential candidates
for gene delivery applications.

Objectives:
This study aims to explore a range of short synthetic lipopeptides, (Lau: Lauryl; Pal:
Palmitoyl) consisting of an alkyl chain, one cysteine (C), 1 to 2 histidine (H), and lysine (K) residues
by performing in-silico molecular interaction and in-vitro evaluation.

Methods:
The molecular interactions between the lipopeptides and Importin-α receptor were performed
using AutoDock Vina and Amber14. The lipopeptide/DNA complexes were evaluated in-
-vitro for their interactions, particle size, zeta potential and transgene expression. Transfection efficiency
of the lipopeptides and Pal-CKKHH-derived liposome was carried out based on luciferase
transgene expression.

Results:
The in-silico interaction showed that Lau-CKKH and Pal-CKKHH hypothetically expedited
nuclear uptake. Both lipopeptides had lower binding energy (-6.3 kcal/mol and -6.2 kcal/mol, respectively),
compared to the native ligand, viz, nuclear localization sequence (-5.4 kcal/mol). The
short lipopeptides were able to condense DNA molecules and efficiently form compacted nanoparticles.
Based on the in-vitro evaluation on COS-7, Pal-CKKHH was found to be the best transfection
agent amongst the lipopeptides. Its transfection efficiency (ng Luc/mg total protein) increased up to
~3-fold higher (1163 + 55) as it was formulated with helper lipid DOPE (1:2). The lipopeptide-
based liposome (Pal-CKKHH: DOPE=1:2) also facilitated luciferase transgene expression on
human embryonic kidney cells (293T) and human cervical adenocarcinoma cells (HeLa) with transfection
efficiency 1779 +52 and 260 + 22, respectively.

Conclusion:
Our study for the first time has shown that the fully synthesized short lipopeptide Pal-
CKKHH is able to interact firmly with the Importin-α. The lipopeptide is able to condense DNA
molecules efficiently, facilitate transgene expression, expedite the nuclear uptake process, and
hence has the characteristics of a potential transfection agent.
PENGARUH EARNING PER SHARE, RETURN ON ASSETS, DAN DEBT TO EQUITY RATIO TERHADAP HARGA SAHAM
Authors: Dika Muhammad Fala , Pasaribu Hiras 
Published: October 2020

Abstrak: Pengaruh Earning Per Share, Return On Assets, dan Debt to Equity Ratio Terhadap Harga Saham. Penelitian ini berujuan, untuk mengetahui pengaruh Earning Per Share (EPS), Return On Assets (ROA), dan Debt to Equity Ratio (DER) dari, ROA terhadap harga saham. Penelitian ini dilakukan pada perusahaan industri makanan dan minuman yang terdaftar di Bursa Efek Indonesia periode tahun 2015 – 2018 sebanyak 19 perusahaan. Teknik penarikan sampel menggunakan metode purposive sampling, dengan kriteria yang ditentukan dalam penelitian ini. Sesuai kriteria penarikan sampel diperoleh sampel jumlah 13 perusahaan Metode analisis data menggunakan analisis regresi berganda. Hasil penelitian ini menunjukkan variable EPS dan ROA berpengaruh signifikan terhadap harga saham, sedangkan DER tidak berpengaruh signifikan terhadap harga saham. Kata Kunci: EPS, ROA, DER , Harga Saham.
Combination Treatment with Sodium Nitrite and Isoquercetin on Endothelial Dysfunction among Patients with CKD
Authors: Chen Jing , Hamm L. Lee , Bundy Joshua D. , Kumbala Damodar R. , Bodana Shirisha , Chandra Sehgal , Chen Chung-Shiuan , Starcke Charlton C. , Guo Yajun , Schaefer Caroline M. , Lustigova Eva , Mahone Erin , Vadalia Aarti M. , Livingston Terra , Obst Katherine , Hernandez Jesus , Bokhari Syed Rizwan , Kleinpeter Myra , Alper Arnold B. , Lukitsch Ivo , He Hua , Nieman David C. , He Jiang 
Published: October 2020

Background and objectives
Endothelial dysfunction is common among patients with CKD. We tested the efficacy and safety of combination treatment with sodium nitrite and isoquercetin on biomarkers of endothelial dysfunction in patients with CKD.

Design, setting, participants, & measurements
This randomized, double-blind, placebo-controlled phase 2 pilot trial enrolled 70 patients with predialysis CKD. Thirty-five were randomly assigned to combination treatment with sodium nitrite (40 mg twice daily) and isoquercetin (225 mg once daily) for 12 weeks, and 35 were randomly assigned to placebo. The primary outcome was mean change in flow-mediated vasodilation over the 12-week intervention. Secondary and safety outcomes included biomarkers of endothelial dysfunction, inflammation, and oxidative stress as well as kidney function, methemoglobin, and adverse events. Intention-to-treat analysis was conducted.

Results
Baseline characteristics, including age, sex, race, cigarette smoking, history of hypertension and diabetes, use of renin-angiotensin system blockers, BP, fasting glucose, lipid profile, kidney function, urine albumin-creatinine ratio, and endothelial biomarkers, were comparable between groups. Over the 12-week intervention, flow-mediated vasodilation increased 1.1% (95% confidence interval, −0.1 to 2.3) in the treatment group and 0.3% (95% confidence interval, −0.9 to 1.5) in the placebo group, and net change was 0.8% (95% confidence interval, −0.9 to 2.5). In addition, changes in biomarkers of endothelial dysfunction (vascular adhesion molecule-1, intercellular adhesion molecule-1, E-selectin, vWf, endostatin, and asymmetric dimethylarginine), inflammation (TNF-α, IL-6, C-reactive protein, IL-1 receptor antagonist, and monocyte chemoattractant protein-1), and oxidative stress (oxidized LDL and nitrotyrosines) were not significantly different between the two groups. Furthermore, changes in eGFR, urine albumin-creatinine ratio, methemoglobin, and adverse events were not significantly different between groups.

Conclusions
This randomized phase 2 pilot trial suggests that combination treatment with sodium nitrite and isoquercetin did not significantly improve flow-mediated vasodilation or other endothelial function biomarkers but also did not increase adverse events compared with placebo among patients with CKD.

Clinical Trial registry name and registration number:
Nitrite, Isoquercetin, and Endothelial Dysfunction (NICE), NCT02552888