bluuebunny/crossref_metadata_2025_split huggingface dataset:
Differential clusterization of soluble and extracellular vesicle-associated cytokines in myocardial infarction
Authors: Lebedeva Anna , Fitzgerald Wendy , Molodtsov Ivan , Shpektor Alexander , Vasilieva Elena , Margolis Leonid 
Published: December 2020

AbstractA proinflammatory dysregulation of cytokine release is associated with various diseases, in particular with those of infectious etiology, as well as with cardiovascular diseases (CVD). We showed earlier that cytokines are released in two forms, soluble and in association with extracellular vesicles (EVs). Here, we investigated the patterns of expression and clustering of soluble and EV-associated cytokines in patients with ST-elevation myocardial infarction (STEMI). We collected plasma samples from 48 volunteers without CVD and 62 patients with STEMI, separated soluble and EV fractions, and analyzed them for 33 cytokines using a multiplexed bead-based assay. We identified soluble and EV-associated cytokines that are upregulated in STEMI and form correlative clusters. Several clustered soluble cytokines were expressed almost exclusively in patients with STEMI. EV-associated cytokines were largely not affected by STEMI, except for pro-inflammatory cytokines IL-6, IL-18, and MIG, as well as anti-inflammatory IL-2 that were upregulated in a correlated fashion. Our results demonstrated that soluble cytokines in patients with STEMI are upregulated in a coordinated fashion in contrast to the mainly unaffected system of EV-associated cytokines. Identification of cytokine clusters affected differently by STEMI now permits investigation of their differential contributions to this pathology.
Viral clearance after early corticosteroid treatment in patients with moderate or severe covid-19
Authors: Spagnuolo V. , Guffanti M. , Galli L. , Poli A. , Querini P. Rovere , Ripa M. , Clementi M. , Scarpellini P. , Lazzarin A. , Tresoldi M. , Dagna L. , Zangrillo A. , Ciceri F. , Castagna A. ,   COVID-BioB study group, Andolina A. , Redaelli M. Baiardo , Baldissera E. , Bigai G. , Bigoloni A. , Boffini N. , Borio G. , Bossolasco S. , Bruzzesi E. , Calabrò M. G. , Calvisi S. , Campochiaro C. , Canetti D. , Canti V. , Castellani J. , Castiglioni B. , Cavalli G. , Cavallo L. , Cernuschi M. , Chiurlo M. , Cilla M. , Cinel E. , Cinque P. , Conte C. , Da Prat V. , Danise A. , De Lorenzo R. , De Luca G. , Dell’Acqua A. , Dell’Acqua R. , Della-Torre E. , Torre L. Della , Di Terlizzi G. , Dumea I. , Farolfi F. , Ferrante M. , Frangi C. , Fumagalli L. , Gallina G. , Germinario B. , Gianotti N. , Hasson H. , Lalla F. , Landoni G. , Lanzillotta M. , Voti R. Li , Mastrangelo A. , Messina E. , Moizo E. , Montagna M. , Monti G. , Morsica G. , Muccini C. , Nozza S. , Oltolini C. , Pascali M. , Patrizi A. , Pieri M. , Prestifilippo D. , Ramirez G. , Ranzenigo M. , Sapienza J. , Sartorelli S. , Seghi F. , Tambussi G. , Din C. Tassan , Tomelleri A. , Turi S. , Uberti-Foppa C. , Vinci C. 
Published: December 2020

AbstractThe aim of this study was to evaluate the impact of early treatment with corticosteroids on SARS-CoV-2 clearance in hospitalized COVID-19 patients. Retrospective analysis on patients admitted to the San Raffaele Hospital (Milan, Italy) with moderate/severe COVID-19 and availability of at least two nasopharyngeal swabs. The primary outcome was the time to nasopharyngeal swab negativization. A multivariable Cox model was fitted to determine factors associated with nasopharyngeal swab negativization. Of 280 patients included, 59 (21.1%) patients were treated with steroids. Differences observed between steroid users and non-users included the proportion of patients with a baseline PaO2/FiO2 ≤ 200 mmHg (45.8% vs 34.4% in steroids and non-steroids users, respectively; p = 0.023) or ≤ 100 mmHg (16.9% vs 12.7%; p = 0.027), and length of hospitalization (20 vs 14 days; p < 0.001). Time to negativization of nasopharyngeal swabs was similar in steroid and non-steroid users (p = 0.985). According to multivariate analysis, SARS-CoV-2 clearance was associated with age ≤ 70 years, a shorter duration of symptoms at admission, a baseline PaO2/FiO2 > 200 mmHg, and a lymphocyte count at admission > 1.0 × 109/L. SARS-CoV-2 clearance was not associated with corticosteroid use. Our study shows that delayed SARS-CoV-2 clearance in moderate/severe COVID-19 is associated with older age and a more severe disease, but not with an early use of corticosteroids.
Abnormal cortical thickening and thinning in idiopathic normal-pressure hydrocephalus
Authors: Kang Kyunghun , Han Jaehwan , Lee Sang-Woo , Jeong Shin Young , Lim Yong-Hyun , Lee Jong-Min , Yoon Uicheul 
Published: December 2020

AbstractWe investigated differences in cortical thickness between idiopathic normal-pressure hydrocephalus (INPH) patients and healthy controls. We also explored whether a relationship exists between cortical thinning and gait disturbance in INPH patients. Forty-nine INPH patients and 26 healthy controls were imaged with MRI, including 3-dimensional volumetric images, for automated surface-based cortical thickness analysis across the entire brain. Compared with age- and gender-matched healthy controls, unexpectedly, INPH patients showed statistically significant cortical thickening mainly in areas located in the high convexity of the frontal, parietal, and occipital regions. Additionally, cortical thinning mainly in temporal and orbitofrontal regions was observed in the INPH group relative to the control group. The Gait Status Scale (GSS) scores were negatively correlated with cortical thickness in the medial orbital part of the superior frontal gyrus, gyrus rectus, superior temporal gyrus, temporal pole, and insula. A distinctive pattern of cortical thickness changes was found in INPH patients. We cautiously suggest that cortical thickening in INPH can result from reactive gliosis. Further, our results support the hypothesis that cortical thinning in INPH can result from neuronal degeneration. In addition, cortical thinning can play an important role in gait disturbances in INPH patients.
Advanced co-culture 3D breast cancer model for investigation of fibrosis induced by external stimuli: optimization study
Authors: Yakavets Ilya , Francois Aurelie , Benoit Alice , Merlin Jean-Louis , Bezdetnaya Lina , Vogin Guillaume 
Published: December 2020

AbstractRadiation-induced fibrosis (RIF) is the main late radiation toxicity in breast cancer patients. Most of the current 3D in vitro breast cancer models are composed by cancer cells only and are unable to reproduce the complex cellular homeostasis within the tumor microenvironment to study RIF mechanisms. In order to account complex cellular interactions within the tumor microenvironment, an advanced 3D spheroid model, consisting of the luminal breast cancer MCF-7 cells and MRC-5 fibroblasts, was developed. The spheroids were generated using the liquid overlay technique in culture media into 96-well plates previously coated with 1% agarose (m/v, in water). In total, 21 experimental setups were tested during the optimization of the model. The generated spheroids were characterized using fluorescence imaging, immunohistology and immunohistochemistry. The expression of ECM components was confirmed in co-culture spheroids. Using α-SMA staining, we confirmed the differentiation of healthy fibroblasts into myofibroblasts upon the co-culturing with cancer cells. The induction of fibrosis was studied in spheroids treated 24 h with 10 ng/mL TGF-β and/or 2 Gy irradiation. Overall, the developed advanced 3D stroma-rich in vitro model of breast cancer provides a possibility to study fibrosis mechanisms taking into account 3D arrangement of the complex tumor microenvironment.
Controlled and cellulose eco-friendly synthesis and characterization of Bi2O2CO3 quantum dot nanostructures (QDNSs) and drug delivery study
Authors: Samarehfekri Hojat , Rahimi Hamid Reza , Ranjbar Mehdi 
Published: December 2020

AbstractThis work aimed to prepare solvent-free or green Bi2O2CO3 for quantum dot nanostructures (QDNSs) based on cellulose as a stabilizer and green capping agent to sorafenib delivery for liver targeting. Because the walnut tree is one of the most abundant trees in Iran, it was tried to synthesize Bi2O2CO3 QDNSs using a walnut skin extract. The saturation magnetization for Bi2O2CO3 QDNSs was calculated to be 68.1. Also, the size of products was measured at around 60–80 nm with the Debye–Scherrer equation. Moreover, the morphology, functional groups, and crystallography of the Bi2O2CO3 nanoparticles were investigated using atomic force microscopy, scanning electron microscopy, vibrating-sample magnetometer, and Uv–vis spectroscopy. The results demonstrated that Bi2O2CO3 QDNSs have opto-magnetic properties and they can be suggested as the candidate materials for the sorafenib delivery on the liver tissue. The optical band gap estimated for Bi2O2CO3 QDNSs was found to be red-shift from 3.22 eV. This study suggests the preparation of the Bi2O2CO3 QDNSs based on cellulose as new opto-magnetic materials at different temperatures of 180 °C, 200 °C, 220 °C, and 240 °C for sorafenib delivery as a type of biological therapy drug.
Radiomic features based on Hessian index for prediction of prognosis in head-and-neck cancer patients
Authors: Le Quoc Cuong , Arimura Hidetaka , Ninomiya Kenta , Kabata Yutaro 
Published: December 2020

AbstractThis study demonstrated the usefulness of radiomic features based on the Hessian index of differential topology for the prediction of prognosis prior to treatment in head-and-neck (HN) cancer patients. The Hessian index, which can indicate tumor heterogeneity with convex, concave, and other points (saddle points), was calculated as the number of negative eigenvalues of the Hessian matrix at each voxel on computed tomography (CT) images. Three types of signatures were constructed in a training cohort (n = 126), one type each from CT conventional features, Hessian index features, and combined features from the conventional and index feature sets. The prognostic value of the signatures were evaluated using statistically significant difference (pvalue, log-rank test) to compare the survival curves of low- and high-risk groups. In a test cohort (n = 68), thepvalues of the models built with conventional, index, combined features, and clinical variables were 2.95$$\times$$×10–2, 1.85$$\times$$×10–2, 3.17$$\times$$×10–2, and 1.87$$\times$$×10–3, respectively. When the features were integrated with clinical variables, thepvalues of conventional, index, and combined features were 3.53$$\times$$×10–3, 1.28$$\times$$×10–3, and 1.45$$\times$$×10–3, respectively. This result indicates that index features could provide more prognostic information than conventional features and further increase the prognostic value of clinical variables in HN cancer patients.
The matrix metalloproteinase inhibitor marimastat inhibits seizures in a model of kainic acid-induced status epilepticus
Authors: Pijet Barbara , Konopka Anna , Rejmak Emilia , Stefaniuk Marzena , Khomiak Danylo , Bulska Ewa , Pikul Stanisław , Kaczmarek Leszek 
Published: December 2020

AbstractAn intra-hippocampus injection of kainic acid serves as a model of status epilepticus and the subsequent development of temporal lobe epilepsy. Matrix metalloproteinase-9 (MMP-9) is an enzyme that controls remodeling of the extracellular milieu under physiological and pathological conditions. In response to brain insult, MMP-9 contributes to pathological synaptic plasticity that may play a role in the progression of an epileptic condition. Marimastat is a metalloproteinase inhibitor that was tested in clinical trials of cancer. The present study assessed whether marimastat can impair the development of epilepsy. The inhibitory efficacy of marimastat was initially tested in neuronal cultures in vitro. As a marker substrate, we used nectin-3. Next, we investigated the blood–brain barrier penetration of marimastat using mass spectrometry and evaluated the therapeutic potential of marimastat against seizure outcomes. We found that marimastat inhibited the cleavage of nectin-3 in hippocampal neuronal cell cultures. Marimastat penetrated the blood–brain barrier and exerted an inhibitory effect on metalloproteinase activity in the brain. Finally, marimastat decreased some seizure parameters, such as seizure score and number, but did not directly affect status epilepticus. The long-term effects of marimastat were evident up to 6 weeks after kainic acid administration, in which marimastat still inhibited seizure duration.
Neural changes in early visual processing after 6 months of mindfulness training in older adults
Authors: Isbel Ben , Weber Jan , Lagopoulos Jim , Stefanidis Kayla , Anderson Hannah , Summers Mathew J. 
Published: December 2020

AbstractMindfulness has been shown to improve attentional performance, which is known to decline in aging. Long-latency electroencephalographic (EEG) event-related potential (ERP) changes have been reported immediately after mindfulness training, however the enduring stability of these effects is unknown. Furthermore, the ability of mindfulness to impact earlier stages of information processing is unclear. We examined neural activation using high density EEG in older adults engaged in mindfulness training to examine the long-term stability of training effects. After 6 months of training, mindfulness practitioners displayed enhanced neural activation during sensory encoding and perceptual processing of a visual cue. Enhanced perceptual processing of a visual cue was associated with increased neural activation during post-perceptual processing of a subsequent target. Similar changes were not observed in a control group engaged in computer-based attention training over the same period. Neural changes following mindfulness training were accompanied by behavioural improvements in attentional performance. Our results are suggestive of increased efficiency of the neural pathways subserving bottom-up visual processing together with an enhanced ability to mobilise top-down attentional processes during perceptual and post-perceptual processing following mindfulness training. These results indicate that mindfulness may enhance neural processes known to deteriorate in normal aging and age-related neurodegenerative diseases.
Interactions between snow cover and evaporation lead to higher sensitivity of streamflow to temperature
Authors: Meira Neto Antônio Alves , Niu Guo-Yue , Roy Tirthankar , Tyler Scott , Troch Peter A. 
Published: December 2020

AbstractEstimates of potential evaporation often neglect the effects of snow cover on evaporation process. Here, we present a definition of potential evaporation that explicitly accounts for landscapes that are partially covered by snow. We show that, in the presence of snowpack, our evaporation estimates differ from conventional methods that assume evaporation from a free water surface. Specifically, we find that conventional methods overestimate potential evaporation as well as aridity, taken as the ratio of atmospheric water demand to supply, in landscapes where snowfall is significant. With dwindling snow-cover, actual aridity increases, which could explain the reduction in streamflow with decreasing snowfall. We suggest that streamflow, and hence water availability, is more sensitive to temperature changes in colder than in warmer regions.
Search for radio jets from massive young stellar objects
Authors: Kavak Ü. , Sánchez-Monge Á. , López-Sepulcre A. , Cesaroni R. , van der Tak F. F. S. , Moscadelli L. , Beltrán M. T. , Schilke P. 
Published: December 2020

Context.Recent theoretical and observational studies debate the similarities of the formation process of high- (>8M⊙) and low-mass stars. The formation of low-mass stars is directly associated with the presence of disks and jets. Theoretical models predict that stars with masses up to 140M⊙can be formed through disk-mediated accretion in disk-jet systems. According to this scenario, radio jets are expected to be common in high-mass star-forming regions.Aims.We aim to increase the number of known radio jets in high-mass star-forming regions by searching for radio-jet candidates at radio continuum wavelengths.Methods.We used theKarl G. JanskyVery Large Array (VLA) to observe 18 high-mass star-forming regions in theCband (6 cm, ≈1′′.0 resolution) andKband (1.3 cm, ≈0′′.3 resolution). We searched for radio-jet candidates by studying the association of radio continuum sources with shock activity signs (e.g., molecular outflows, extended green objects, and maser emission). Our VLA observations also targeted the 22 GHz H2O and 6.7 GHz CH3OH maser lines.Results.We have identified 146 radio continuum sources, 40 of which are located within the field of view of both images (CandKband maps). We derived the spectral index, which is consistent with thermal emission (between − 0.1 and + 2.0) for 73% of these sources. Based on the association with shock-activity signs, we identified 28 radio-jet candidates. Out of these, we identified 7 as the most probable radio jets. The radio luminosity of the radio-jet candidates is correlated with the bolometric luminosity and the outflow momentum rate. About 7–36% of the radio-jet candidates are associated with nonthermal emission. The radio-jet candidates associated with 6.7 GHz CH3OH maser emission are preferentially thermal winds and jets, while a considerable fraction of radio-jet candidates associated with H2O masers show nonthermal emission that is likely due to strong shocks.Conclusions.About 60% of the radio continuum sources detected within the field of view of our VLA images are potential radio jets. The remaining sources could be compact H IIregions in their early stages of development, or radio jets for which we currently lack further evidence of shock activity. Our sample of 18 regions is divided into 8 less evolved infrared-dark regions and 10 more evolved infrared-bright regions. We found that ≈71% of the identified radio-jet candidates are located in the more evolved regions. Similarly, 25% of the less evolved regions harbor one of the most probable radio jets, while up to 50% of the more evolved regions contain one of these radio-jet candidates. This suggests that the detection of radio jets in high-mass star-forming regions is more likely in slightly more evolved regions.
Weighing the Galactic disk in sub-regions of the solar neighbourhood using Gaia DR2
Authors: Widmark A. , de Salas P. F. , Monari G. 
Published: December 2020

Aims. We infer the gravitational potential of the Galactic disk by analysing the phase-space densities of 120 stellar samples in 40 spatially separate sub-regions of the solar neighbourhood, using Gaia’s second data release (DR2), in order to quantify spatially dependent systematic effects that bias this type of measurement.
Methods. The gravitational potential was inferred under the assumption of a steady state in the framework of a Bayesian hierarchical model. We performed a joint fit of our stellar tracers’ three-dimensional velocity distribution, while fully accounting for the astrometric uncertainties of all stars as well as dust extinction, and we also masked angular areas of known open clusters. The inferred gravitational potential is compared, post-inference, to a model for the baryonic matter and halo dark matter components.
Results. We see an unexpected but clear trend for all 40 spatially separate sub-regions: Compared to the potential derived from the baryonic model, the inferred gravitational potential is significantly steeper close to the Galactic mid-plane (≲60 pc), but flattens such that the two agree well at greater distances (∼400 pc). The inferred potential implies a total matter density distribution that is highly concentrated to the Galactic mid-plane and decays quickly with height. We see a dependence on the Galactic radius that is consistent with a disk scale length of a few kiloparsecs. Apart from this, there are discrepancies between stellar samples, implying spatially dependent systematic effects which are, at least in part, explained by substructures in the phase-space distributions.
Conclusions. In terms of the inferred matter density distribution, the very low matter density that is inferred at greater heights (≳300 pc) is inconsistent with the observed scale height and matter distribution of the stellar disk, which cannot be explained by a misunderstood density of cold gas or other hidden mass. Our interpretation is that these results must be biased by a time-varying phase-space structure, possibly a breathing mode, that is large enough to affect all stellar samples in the same manner.
Obesity in Gynecologic Oncology
Authors: Kölbl Heinz , Bartl Thomas 
Published: December 2020

AbstractThe decades-long global obesity epidemic has resulted in steady increase in the incidence of obesity-related malignancies. The associated diagnostic and therapeutic implications present a clinical challenge for gynecologic oncology treatment strategies. Recent studies have provided solid evidence for an independent, linear, positive correlation between a pathologically increased body mass index and the probability of developing endometrial or postmenopausal breast cancer. The pathogenesis is complex and the subject of current research. Proposed causes include pathologically increased serum levels of sexual steroids and adiponectin, obesity-induced insulin resistance, and systemic inflammatory processes. The scientific evidence for an association between obesity and other gynecological malignancies is, however, less solid. The clinical relevance of obesity as a risk factor for epithelial ovarian cancer, cervical cancer and vulvar cancer appears to be negligible.
Nevertheless, obesity appears to have a negative impact on prognosis and oncologic outcomes for all gynecological cancers. Whether or not this effect can be interpreted as correlative or causal is still a subject of ongoing debate.
Status of Sentinel Lymph Node Biopsy in Vulvar and Cervical Cancer
Authors: Waldschmidt Julia , Jung Lisa , Juhasz-Böss Ingolf 
Published: December 2020

AbstractAssessment of lymphatic metastasis is an essential component of solid tumour staging. Sentinel lymph node (SLN) biopsy is a minimally invasive procedure that allows regional lymph node involvement by tumour to be estimated by selectively examining the sentinel lymph node while minimising the morbidity of systematic lymph node dissection. Within the group of genital cancers, the diagnostic value of SLN biopsy is rated differently. For selected patients with early-stage vulvar cancer (unifocal primary tumour < 4 cm, clinically negative inguinal lymph nodes) the SLN technique is already an established procedure in the guidelines of the German Society for Gynaecology and Obstetrics (DGGG)/German Cancer Society (DKG) and the recommendations of the European Society of Gynaecological Oncology (ESGO). For cervical cancer, SLN biopsy has not yet been sufficiently standardised but can be considered for patients without risk factors with a primary tumour size < 2 cm. The SLN
is identified by combined use of radioactive 99mtechnetium nanocolloid and patent blue. The use of indocyanine green offers an alternative for SLN identification with few side effects. Recent studies aim to increase the diagnostic reliability of intraoperative frozen section analysis as this continues to show limited sensitivity in both vulvar and cervical cancer. The rate of detection of micrometastases can be increased by additional ultrastaging, the prognostic significance of which for both diseases is still unclear. The prognostic value of SLN biopsy compared with systematic lymph node dissection is being investigated in current studies (GROINSS-V-II for vulvar cancer and SENTIX-, SENTICOL-3 for cervical cancer). For this review article, a guideline-based literature search was performed in the National Library of Medicine (PubMed/MEDLINE) database with a particular focus on recent cohort studies and conference contributions.