bluuebunny/crossref_metadata_2025_split huggingface dataset:
Unidirectional response to bidirectional selection on body size II Quantitative genetics
Authors: Rouzic Arnaud Le , Renneville Clémentine , Millot Alexis , Agostini Simon , Carmignac David , Édeline Éric 
Published: January 2020

AbstractAnticipating the genetic and phenotypic changes induced by natural or artificial selection requires reliable estimates of trait evolvabilities (genetic variances and covariances). However, whether or not multivariate quantitative genetics models are able to predict precisely the evolution of traits of interest, especially fitness-related, life-history traits, remains an open empirical question. Here, we assessed to what extent the response to bivariate artificial selection on both body size and maturity in the medakaOryzias latipes, a model fish species, fits the theoretical predictions. Three lines (Large, Small, and Control lines) were differentially selected for body length at 75 days of age, conditional on maturity. As maturity and body size were phenotypically correlated, this selection procedure generated a bi-dimensional selection pattern on two life history traits. After removal of non-heritable trends and noise with a random effect (’animal’) model, the observed selection response did not match the expected bidirectional response. For body size, Large and Control lines responded along selection gradients (larger body size and stasis, respectively), but, surprisingly, the Small did not evolve a smaller body length, and remained identical to the Control line throughout the experiment. The magnitude of the empirical response was smaller than the theoretical prediction in both selected directions. For maturity, the response was opposite to the expectation (the Large line evolved late maturity compared to the Control line, while the Small line evolved early maturity, while the opposite pattern was predicted due to the strong positive genetic correlation between both traits). The mismatch between predicted and observed response was substantial and could not be explained by usual sources of uncertainties (including sampling effects, genetic drift, and error in G matrix estimates).
Genome-Wide Identification, Characterization and Expression Analysis of Non-Arginine Aspartate Receptor like kinase gene family underColletotrichum truncatumstress conditions in Hot pepper
Authors: Srideepthi R , Krishna MSR , Suneetha P , Krishna R Sai , Karthikeyan S 
Published: January 2020

AbstractReceptor Like kinases (RLKs) are conserved upstream signaling molecules that regulate several biological processes, including plant development and stress adaptation. Non arginine aspartate (non-RD) an important class of RLKs plays a vital role in disease resistance and apoptosis in plants. In present investigation, a comprehensive Insilco analysis for non-RD Kinase gene family including identification, sequence similarity, phylogeny, chromosomal localization, gene structures, gene duplication analysis, promoter analysis and transcript expression profiles were elucidated. In this study twenty six genes were observed on nine out of twelve chromosomes. All these genes were clustered into seven subfamilies under large monophyletic group termed as Interleukin-1 Receptor-Associated Kinase (IRAK) family. Structural diversity in genomic structure among non-RD kinase gene family were identified and presence of pathogen inducedcisregulatory elements like STRE, MYC, MYB,W box were found. Expression profiles of genes involved in providing resistance to anthracnose pathogenColletotrichum truncatumin hot pepper were analyzed at different infective stages in both resistant and susceptible genotypes. Among twenty six genes,CaRLK1gene belonging to LRRXII subfamily was up regulated under severe stress after infection in resistant genotype PBC-80. This integrative approach has helped us to identify candidate genes involved in disease resistance which would be helpful in future crop improvement programs.
Effects of sustained inflation pressure during neonatal cardiopulmonary resuscitation of asphyxiated piglets
Authors: Shim Gyu-Hong , Kim Seung Yeon , Cheung Po-Yin , Lee Tze-Fun , O’Reilly Megan , Schmölzer Georg M 
Published: January 2020

AbstractObjectiveSustained inflation (SI) during chest compression (CC = CC+SI) significantly reduces time to return of spontaneous circulation (ROSC) compared to 3:1 compression-to-ventilation ratio during neonatal resuscitation. However, the optimal peak inflation pressure (PIP) of SI during CC+SI to improve ROSC and hemodynamic recovery in severely asphyxiated piglets is unknown.AimTo examine if different PIPs of SI during CC+SI will improve ROSC and hemodynamic recovery in severely asphyxiated piglets.Intervention and measurementsTwenty-nine newborn piglets (1-3 days old) were anesthetized, intubated, instrumented and exposed to 30-min normocapnic hypoxia followed by asphyxia. Piglets were randomized into four groups: CC+SI with a PIP of 10 cmH2O (CC+SI_PIP_10, n=8), a PIP of 20 cmH2O (CC+SI_PIP_20, n=8), a PIP of 30 cmH2O (CC+SI_PIP_30, n=8), and a sham-operated control group (n=5). Heart rate, arterial blood pressure, carotid blood flow, cerebral oxygenation, and respiratory parameters were continuously recorded throughout the experiment.Main resultsBaseline parameters were similar between all groups. There was no difference in asphyxiation (duration and degree) between intervention groups. PIP correlated positively with tidal volume and inversely with exhaled CO2 during cardiopulmonary resuscitation. Time to ROSC and rate of ROSC were similar between piglets resuscitated with CC+SI_PIP_10, CC+SI_PIP_20, and CC+SI_PIP_30 cmH2O: median (IQR) 75 (63-193) sec, 94 (78-210) sec, and 85 (70-90) sec; 5/8 (63%), 7/8 (88%), and 3/8 (38%) (p=0.56 and p=0.12, respectively). All piglets that achieved ROSC survived to four hours post-resuscitation. Piglets resuscitated with CC+SI_PIP_30 cmH2O exhibited increased concentrations of pro-inflammatory cytokines interleukin-1β and tumour necrosis factor-α in the frontoparietal cerebral cortex (both p<0.05 vs. sham-operated controls).ConclusionsIn asphyxiated term newborn piglets resuscitated by CC+SI, the use of different PIPs resulted in similar time to ROSC, but PIP at 30 cmH2O showed a larger VT delivery, lower exhaled CO2 and increased tissue inflammatory markers in the brain.
Stereo-EEG reveals rich cortical dynamics in humans coping with difficult action discrimination
Authors: Platonov A. , Pelliccia V. , Sartori I. , LoRusso G. , Avanzini P. , Orban G.A. 
Published: January 2020

AbstractVisual perception of others’ actions is important for social interactions, and the ability to do so, even when one gets only brief glimpses of others’ behavior, may be crucial for survival. At present it is unknown how the human brain solves this problem. Imaging studies have promoted the idea that the multiple demand (MD) system, a domain general system of the human brain, operates in difficult cognitive and perceptual tasks, but not in tasks in which sensory information is reduced. Functional imaging, based on slow hemodynamic responses, may miss or standardize neural events with very diverse time courses. Here we exploited the temporal resolution of stereo-EEG to study directly cortical activity when human subjects must judge the actions of others, but only get brief glimpses of others’ activity, because the videos were truncated. Multiple cortical regions increased their activity in the difficult action discrimination, relative to the easy task when the complete video was visible. The majority of these regions belonged to the MD system, being located in parietal or prefrontal cortex. The variety of time courses, lasting from a few 100ms to several seconds, allowed us to disentangle control from effector regions, the latter processing observed actions. This distinction was further supported by relationships with behavior. A key operation within the control clusters was the prediction of erroneous responses, which was initiated in the PPC soon after the end of the truncated video. The time courses further suggested that MD regions not only exert control, but also perform various evaluations of the effort, important for efficient and intelligent behavior. We observed also increases outside the MD system, in temporo-parietal cortex, which may provide contextual information about variables related to the observed action, such as the actor, the object or the scene. Furthermore, to cope with the brief sensory input, the MD system called upon warning regions reacting to the static picture of the actor presented just before the video. We conclude that discrimination of brief observed actions indeed involves the MD system, which is thus is more general than assumed so far. WE also show that the MD system is more complex than assumed, as it includes evaluation of control, and more flexible, as it interacts with other systems than simply the effector circuit of the task.
Hox-dependent coordination of cardiac progenitor cell patterning and differentiation
Authors: Stefanovic Sonia , Laforest Brigitte , Desvignes Jean-Pierre , Lescroart Fabienne , Argiro Laurent , Maurel-Zaffran Corinne , Salgado David , de Bono Christopher , Pazur Kristijan , Théveniau-Ruissy Magali , Béroud Christophe , Pucéat Michel , Gavalas Anthony , Kelly Robert G. , Zaffran Stéphane 
Published: January 2020

SUMMARYPerturbation of addition of second heart field (SHF) cardiac progenitor cells to the poles of the heart tube results in congenital heart defects (CHD). The transcriptional programs and upstream regulatory events operating in different subpopulations of the SHF remain unclear. Here, we profile the transcriptome and chromatin accessibility of anterior and posterior SHF sub-populations at genome-wide levels and demonstrate that Hoxb1 negatively regulates differentiation in the posterior SHF. Spatial mis-expression ofHoxb1in the anterior SHF results in hypoplastic right ventricle. Activation ofHoxb1in embryonic stem cells arrests cardiac differentiation, whereasHoxb1-deficient embryos display premature cardiac differentiation. Moreover, ectopic differentiation in the posterior SHF of embryos lacking bothHoxb1and its paralogHoxa1results in atrioventricular septal defects. Our results show that Hoxb1 plays a key role in patterning cardiac progenitor cells that contribute to both cardiac poles and provide new insights into the pathogenesis of CHD.
A chemical tool for improved culture of human pluripotent stem cells
Authors: Silpa Laurence , Schuessler Maximilian , Liu Gu , Olivecrona Marcus , Groizard-Payeras Lucia , Couper Elizabeth , Bataille Carole J. R. , Stevenson Mark , Seymour Len W. , Davies Stephen G. , James William S. , Cowley Sally A. , Russell Angela J. 
Published: January 2020

AbstractThe large-scale and cost-effective production of quality-controlled human pluripotent stem cells (hPSC) for use in cell therapy and drug discovery requires chemically-defined xenobiotic-free culture systems that enable easy and homogeneous expansion of pluripotent cells. Through phenotypic screening, we have identified a small molecule, OXS8360 (an optimized derivative of (-)-Indolactam V ((-)-ILV)), that stably disrupts hPSC cell-cell contacts. Proliferation of hPSC in OXS8360 is normal, as are pluripotency signatures, directed differentiation to hallmark lineages and karyotype over extended passaging. In 3D culture, OXS8360-treated hPSC form smaller, more uniform aggregates, that are easier to dissociate, greatly facilitating expansion. The mode of action of OXS8360 involves disruption of the localisation of the cell-cell adhesion molecule E-cadherin, via activation of unconventional Protein Kinase C isoforms. OXS8360 media supplementation is therefore able to yield more uniform, disaggregated 2D and 3D hPSC cultures, providing the hPSC field with an affordable tool to improve hPSC quality and scalability.
Value co-creation in firm sponsored online communities
Authors: Priharsari Diah , Abedin Babak , Mastio Emmanuel 
Published: January 2020

PurposeThe purpose of this paper is to explore enablers and constraints in value co-creation in sponsored online communities, and to identify firm roles in shaping value co-creation. The structured analysis is translated into strategies for practitioners and for guiding future research.Design/methodology/approachThe authors systematically review and synthesise the literature to develop a comprehensive model of value co-creation.FindingsThe literature review findings have led to the identification of four actors in sponsored online communities, revealed enablers and constraints for value co-creation in online communities, and provided insight into the simultaneous roles of sponsoring firm (co-creator and facilitator) and the interrelationship between them.Research limitations/implicationsLike other systematic literature review studies, the findings are limited by what was reported in the papers selected for the review. The authors contribute to service-dominant logic (SDL) by bridging the macro level to the empirical level, and add to our understanding of the sociomateriality theory by capturing constraints and enablers coming from various actors.Practical implicationsThe extracted enablers and constraints guide decision makers to better design, asses, monitor and support sponsored online communities. The findings also inform how to orchestrate the two sponsoring firm roles so that the online community is still attractive for the members and creates value for the sponsoring firm.Originality/valueGiven the variety of disciplines dealing with value co-creation, and given the plenitude of definitions and related concepts, this study consolidates the existing knowledge and models how value is co-created in online communities.
Examining the influence of factors critical for the consumer to accept the offered Islamic home financing
Authors: Amin Hanudin 
Published: January 2020

PurposeThis study aims to examine the impacts ofShariah-compliant products, value-added benefits, mortgage repayment policy and well-versed bankers on the consumer acceptance of Islamic home financing. These variables, thereafter, are called the “critical success factors” (the CSFs) for Islamic home financing.Design/methodology/approachGuided by the Islamic theory of consumer behaviour (ITCB), this study intends to propose the specific CSFs that ensure consumer acceptance of Islamic home financing a success. The term success refers to consumers’ willingness to take up the facility. A questionnaire survey was conducted to elicit responses pertinent to the level of agreement/disagreement of the CSFs. A total of 460 respondents usable responses were obtained and analysed accordingly.FindingsThe results obtained indicate thatShariah-compliant products, value-added benefits, mortgage repayment policy and well-versed bankers are instrumental to influence the consumer acceptance of Islamic home financing.Research limitations/implicationsLike other works, this study has confined its context to capture only a limited geographical area, and the study’s contributions are restrained to the variables analysed to a specific context of Islamic home financing in Malaysia.Practical implicationsThe results obtained can become as a yardstick to measure the consumer acceptance of Islamic home financing.Originality/valueNew proposed CSFs can help to address the new demand of Islamic home financing accordingly, where a new area of improvement is brought into play. This study also extends the applicability of the ITCB to include Islamic home financing products.
Understanding customer brand engagement in user-initiated online brand communities: antecedents and consequences
Authors: Matute Jorge , Palau-Saumell Ramon , Occhiocupo Nicoletta 
Published: January 2020

Purpose
The purpose of this study is to provide a better understanding of customer brand engagement (CBE) by proposing and empirically testing a model of antecedents and consequences of CBE for user-initiated online brand communities (OBCs).

Design/methodology/approach
The model is tested using a sample of 584 participants in two relevant OBCs created and managed by brand fans. Specifically, data were collected from two communities in the photography products category: Nikonistas and Canonistas.

Findings
The results indicate that community and brand identification positively and significantly influence CBE. Furthermore, the supporting role of OBCs’ moderators facilitates CBE and moderates the influence of community identification on CBE. Regarding the outcomes of CBE, the results show that higher levels of engagement are positively, directly and significantly associated with favorable intentions towards the brand and the community. These effects are then mediated by brand affective commitment.

Research limitations/implications
The study has been conducted in two Spanish OBCs of two specific high-involvement products category: it is cross-sectional and focuses on a limited number of antecedents and consequences.

Practical implications
Evidence from this research supports and emphasizes the potential that these platforms have for brand management such that firms’ resources could be best allocated on those elements that lead to superior CBE.

Originality/value
The study endorses the role of CBE in fostering brand and community-related favorable outcomes in the context of user-initiated OBCs. It shed lights on the potential that these online platforms have for brands and on the role that brand management should play in digital contexts that are outside the direct control of the company.
Integration versus conflict between schools of dream theory and dreamwork: integrating the psychological core qualities of dreams with the contemporary knowledge of the dreaming brain
Authors: Vedfelt Ole 
Published: January 2020

AbstractThe various ways schools of psychotherapy relate to dreams have been marked by isolationism and mutual conflict rather than self‐examination and then integrating the discoveries and methods of other schools. Jung’s method was in opposition to Freud’s psychoanalysis. Existential psychology was dismissive of Freud’s and Jung’s discoveries, while cognitive dream interpretation and cognitive therapy sought other roads entirely. In addition, scientific and neuropsychological dream research has been only insignificantly tied to the psychotherapeutic dream theories. These conflicts and the lack of a comprehensive dream theory has made it convenient for the current rationalist collective consciousness and treatment systems to reject the often times challenging knowledge about ourselves that dreams can provide.This paper describes how contemporary theories of complex cybernetic information networks can create an overriding, constructive framework for uncovering common traits within the above‐mentioned branches of dream research and dreamwork. Within this framework, tencore qualitiesare delineated, supported by both therapeutic knowledge as well as scientific research: 1) Dreams deal with matters important to us; 2) Dreams symbolize; 3) Dreams personify; 4) Dreams are trial runs in a safe place; 5) Dreams are online to unconscious intelligence; 6) Dreams are pattern recognition; 7) Dreams are high level communication; 8) Dreams are condensed information; 9) Dreams are experiences of wholeness; 10) Dreams are psychological energy landscapes.For each core quality I describe short dreamwork sequences from my own practice and a schematic image of how I perceive the overriding interaction between systems in the dreaming brain. For each core quality recommendations for practical dreamwork are provided.Finally, I draw attention to dreams as a huge psychological resource for humankind.
Parents’ experiences of care following the loss of a baby at the margins between miscarriage, stillbirth and neonatal death: a UK qualitative study
Authors: Smith LK , Dickens J , Bender Atik R , Bevan C , Fisher J , Hinton L 
Published: January 2020

ObjectiveTo explore the healthcare experiences of parents whose baby died either before, during or shortly after birth between 20+0 and 23+6 weeks of gestation in order to identify practical ways to improve healthcare provision.DesignQualitative interview study.SettingEngland through two parent support organisations and four NHS Trusts.SampleA purposive sample of parents.MethodsThematic analysis of semi‐structured in‐depth narrative interviews.Main outcome measuresParents’ healthcare experiences.ResultsThe key overarching theme to emerge from interviews with 38 parents was the importance of the terminology used to refer to the death of their baby. Parents who were told they were ‘losing a baby’ rather than ‘having a miscarriage’ were more prepared for the realities of labour, the birth experience and for making decisions around seeing and holding their baby. Appropriate terminology validated their loss, and impacted on parents’ health and wellbeing immediately following bereavement and in the longer term.ConclusionFor parents experiencing the death of their baby at the margins between miscarriage, stillbirth and neonatal death, ensuring the use of appropriate terminology that reflects parents’ preferences is vital. This helps to validate their loss and prepare them for the experiences of labour and birth. Reflecting parents’ language preferences combined with compassionate bereavement care is likely to have a positive impact on parents’ experiences and improve longer‐term outcomes.Tweetable abstractDescribing baby loss shortly before 24 weeks of gestation as a ‘miscarriage’ does not prepare parents for labour and birth, seeing their baby and making memories.
Optimal conditions for the encapsulation of Weissella cibaria JW15 using alginate and chicory root and evaluation of capsule stability in a simulated gastrointestinal system
Authors: Kim Mina , Nam Dong‐Geon , Im Pureum , Choe Jeong‐Sook , Choi Ae‐Jin 
Published: January 2020

AbstractThe delivery of active probiotic cells in capsules can reduce probiotic cell loss induced by detrimental external factors during digestion. In this study, we determined the optimal conditions for the encapsulation of Weissella cibaria JW15 (JW15) within calcium and polyethylene glycol (PEG)‐alginate with chicory root extract powder (CREP). JW15 was encapsulated as the core material (109 cells/mL, 2 mL/min), and a solution containing a mixture of 1.5% sodium alginate and 1% CREP was extruded into a receiving bath with 0.1 M calcium chloride (CaCl2) and 0.05% PEG. Capsule morphology and size were measured using optical microscopy. The optimal air pressure and frequency vibration for capsules containing alginate only (Al) were 200 mbar and 200 Hz, respectively and 100 mbar and 350 Hz for capsules containing alginate with CREP (Ch), respectively. The voltage for both capsules types was fixed at 1.35 kV. Then, the capsules were incubated in a simulated gastrointestinal (GI) system for 6 hr at 37 °C. The addition of PEG in a CaCl2 hardening solution led to degradation of the Ch capsule (Ch–PEG) and the release of cells into the small intestine vessel in the simulated GI system. By contrast, the cells were trapped within the Al capsules. Based on these data, effective encapsulation using alginate with CREP and PEG can enable JW15 to be released at a targeted anatomical site of activity within the GI system, thereby, enhancing the efficacy of probiotic cells. These protective effects can be leveraged during the development of probiotic products.Practical ApplicationWeissella cibaria JW15 (109 cells/mL) was encapsulated in biodegradable and biocompatible capsules, prepared by mixing 1.5% alginate with 1% chicory root extract powder (CREP) in 0.1 M CaCl2 and 0.05% PEG using an encapsulator. The optimal processing parameters were as follows: pressure, 100 mbar; vibration frequency, 350 Hz; voltage, 1.35 kV; and core flow rate, 2 mL/min. When the resulting capsules were subjected to a simulated gastrointestinal system for 6 hr, the cells were released into the small intestine, and up to 95% cell viability was preserved. These results suggest that capsules made from alginate with CREP and formulated using calcium and PEG are a promising delivery system for probiotic cells.