bluuebunny/crossref_metadata_2025_split huggingface dataset:
Efficient Transgenesis in Caenorhabditis elegans Using Flp Recombinase-Mediated Cassette Exchange
Authors: Nonet Michael L 
Published: June 2020

Abstract
The application of CRISPR technology has greatly facilitated the creation of transgenic Caenorhabditis elegans lines. However, methods to insert multi-kilobase DNA constructs remain laborious even with these advances. Here, I describe a new approach for introducing large DNA constructs into the C. elegans genome at specific sites using a combination of Flp and Cre recombinases. The system utilizes specialized integrated landing sites that express GFP ubiquitously flanked by single loxP, FRT, and FRT3 sites. DNA sequences of interest are inserted into an integration vector that contains a sqt-1 self-excising cassette and FRT and FRT3 sites. Plasmid DNA is injected into the germline of landing site animals. Transgenic animals are identified as Rol progeny, and the sqt-1 marker is subsequently excised with heat shock Cre expression. Integration events were obtained at a rate of approximately one integration per three injected F0 animals—a rate substantially higher than any current approach. To demonstrate the robustness of the approach, I compared the efficiency of the Gal4/UAS, QF (and QF2)/QUAS, tetR(and rtetR)/tetO, and LexA/lexO bipartite expression systems by assessing expression levels in combinations of driver and reporter GFP constructs and a direct promoter GFP fusion each integrated at multiple sites in the genome. My data demonstrate that all four bipartite systems are functional in C. elegans. Although the new integration system has several limitations, it greatly reduces the effort required to create single-copy insertions at defined sites in the C. elegans genome.
Funciones ejecutivas en niños escolarizados con alta y baja creatividad
Authors: Krumm Gabriela Liliana , Arán Filippetti Vanessa , Kimel Evelyn 
Published: June 2020

Objetivo: El presente estudio tuvo como objetivo analizar el perfil de funcionamiento ejecutivo característicoen niños creativos.
Método: Participaron 200 niños y niñas escolarizados de la provincia de Entre Ríos, República Argentina,seleccionados por medio de un muestreo intencional. La creatividad se evaluó por medio de la pruebade figuras del Test de Pensamiento Creativo de Torrance (TTCT), Forma A y la prueba verbal CREA C,inteligencia creativa. Como medida de las Funciones Ejecutivas (FE), se emplearon diferentes pruebaspara valorar cada dominio: (1) subtest de memoria de trabajo del WISC IV; (2) Stroop, test de colores ypalabras, para inhibición; (3) WCST computarizado, para flexibilidad cognitiva reactiva y Fluidez verbal yFive Point Test para fluidez cognitiva espontánea, y (4) Laberintos de Porteus para valorar la planificación.Para estudiar perfiles de funcionamiento ejecutivo se realizaron MANCOVAS ingresando la inteligenciacomo covariable.
Resultados: Los resultados del presente estudio sugieren la existencia de un perfil de FE en niñoscreativos en función de su desempeño en la prueba de figuras del TTCT (F de Hotelling (7, 90) = 3.404;p = .003, η2 parcial = .21) y del CREA C (F de Hotelling (7, 98) = 8.831; p < .001, η2 parcial = .39).
Conclusión: Los hallazgos del presente estudio muestran que los niños con mayor creatividad, tantoen tareas de dibujo como en tareas verbales, presentan un perfil de FE caracterizado por una mayorcapacidad de memoria de trabajo, inhibición y flexibilidad cognitiva espontánea. Sin embargo, pareceríaque la naturaleza de la tarea es un factor que podría modular la contribución de los procesos cognitivosde alto orden al potencial creativo.
relación entre emociones y estrategias de afrontamiento ante la reprobación en estudiantes universitarios.
Authors: Reyes Pérez Verónica , Alcázar-Olán Raúl J. , Collazo Saldaña Ana Dulce Karina , De la Roca Chiapas José María 
Published: June 2020

Objetivo: Identificar las diferencias (hombres y mujeres) y la relación entre emociones y las estrategiasde afrontamiento en estudiantes universitarios cuando reprueban un examen al inicio del ciclo escolar.
Método: Estudio no experimental de tipo transversal con múltiples variables continuas. Participaron1,774 universitarios mexicanos (54 % mujeres y 46 % hombres), media de edad 20.72 (DE = 2.27).Se utilizaron dos instrumentos, a saber, Escala de emociones cuando repruebas: desagrado, culpa,vergüenza, tristeza, miedo, frustración, enojo, angustia, indiferencia (α = .94); y Estrategias de afrontamientocuando repruebas: gritar, culpar a otro, reflexionar, reprobar el examen, buscar asesoría, evitarhablar del tema, evitar pensar en eso, fingir estar bien, estar en redes sociales, escuchar música, salir conamigos, salir solo(a), contarle a mi familia (α = .88).
Resultados: Se obtuvieron diferencias estadísticamente significativas entre mujeres y hombres en todoslos factores de la intensidad de las emociones cuando repruebas. En cuanto a las estrategias de afrontamiento más utilizadas tanto para las mujeres (M = 2.96, DE = 0.66) como en los hombres (M = 2.69,DE = 0.67) fueron las Asertivas y de autoconstructivas y las menos las Agresivo-defensivas; en cuanto alas estrategias de Evasión si bien no mostraron diferencias entre hombres (M = 2.00; DE = 0.78) y mujeres(M = 2.11; DE = 0.84), sin embargo, en las correlaciones de la muestra en general fueron las que tuvieronlas puntuaciones más altas con las emociones (r = .53**), seguidas de las Búsqueda de soluciones(r = .38**) y Asertivas y autocríticas (r = .42**). Al realizar los análisis contrastados entre mujeres (r =50**) hombres (r = .56**) nuevamente las estrategias de Evasión fueron las que más correlacionaroncon los factores emocionales (r = .50**).
Conclusiones: Si bien se encontraron diferencias estadísticamente significativas entre hombres ymujeres en la intensidad de las emociones, y fueron ellas quienes reportaron una mayor intensidad, nose encontraron diferencias en las estrategias de afrontamiento agresivo-defensivas, evasión y búsquedade apoyo; el solicitar ayuda es un indicativo de que son capaces de reconocer que tienen un problemaque sobrepasa sus habilidades en ese momento. Conocer qué emociones experimentan y qué estrategiasde afrontamiento utilizan los universitarios cuando reprueban, proporciona al personal encargadode apoyarlos el poder implementar acciones que les ayuden a afianzar y darle continuidad a su trayectoriaacadémica. Es importante apoyar a los estudiantes, ya que las estrategias de afrontamiento quemostraron mayores correlaciones con las emociones fueron las de evasión, lo cual puede traerles consecuenciasnegativas como el pasar de reprobar un examen a reprobar la asignatura.
Glioblastoma and Increased Survival with Longer Chemotherapy Duration
Authors: Jaoude Dory Abou , Moore Joseph A , Moore Matthew B , Twumasi-Ankrah Philip , Ablah Elizabeth , Moore Dennis F. 
Published: June 2020

Introduction
The five-year survival rate for patients with glioblastoma (GBM) is low at approximately 4.7%. Radiotherapy plus concomitant and adjuvant temozolomide (TMZ) remains the standard of care. The optimal duration of therapy with TMZ is unknown. This study sought to evaluate the survival benefit of two years of treatment.
Methods
This was a retrospective chart review of all patients diagnosed with GBM and treated with TMZ for up to two years between January 1, 2002 and December 31, 2011. The Kaplan-Meier method with log-rank test was used to estimate the progression-free survival (PFS) and the overall survival (OS). The results were compared to historical controls and data from previous clinical trials of patients treated up to one year.
Results
Data from 56 patients with confirmed GBM were evaluated. The OS probability was 54% (SE = 0.068) at one year, 28.3% (SE = 0.064) at two years, 17.8% (SE = 0.059) at three years, and 4% (SE = 0.041) at five years. Seven patients (12.5%) were treated with TMZ for two years. Their median time-to-progression was 28 months (95% CI = 5.0 - 28.0), and they had an increased survival probability at three years compared to other patients (log-rank test χ2 (1, N = 56) = 19.2, p < 0.0001).
Conclusions
There may be an advantage for a longer duration of TMZ therapy among patients with GBM, but the sample size was too small for generalization. A multicenter prospective study is needed to dentify optimal duration of TMZ therapy.
Initial Response of Mature, Experienced Runners to Barefoot Running: Transition to Forefoot Strike
Authors: Mullen Scott M. , Toby E. Bruce , Mar Damon E. , Bechtold Megan , Vopat Bryan G. , Melugin Heath , McIff Terence E. 
Published: June 2020

Introduction
Forefoot strike has been advocated for many runnersbecause of the relatively lower impact and push-off forces comparedto a heel strike. The purpose of this study was to explore the abilityof mature (> 30 years old), experienced runners to transition from aheel foot strike to a forefoot strike when first introduced to barefootrunning on a treadmill. We hypothesized: 1) mature runners who heelstrike while wearing traditional training shoes would persist in heelstriking immediately following a switch to barefoot, 2) mean shoeheel-to-toe drop would be significantly greater in runners who persistin heel striking when running barefoot compared to those who transitionto a forefoot strike pattern, and 3) there would be a significantdecrease in heel striking in the barefoot condition as running speedsincreased.
Methods
This was a controlled crossover laboratory study. Thirty-three experienced runners (average 23.4 miles per week) withan average age of 45.6 years were recruited for this study. The participantsfirst ran in their standard running shoes and subsequentlybarefoot. A motion capture system was utilized to detect and analyzeany transition from heel strike to forefoot strike made by study participants.
Results
Of the 26 participants who were classified as heel strikerunners in their running shoes, 50% (13/26, p = 0.001) transitionedto forefoot strike when changing from running in shoes to runningbarefoot.
Conclusions
The injuries associated with transition from standardrunning shoes to barefoot running or minimalist shoes may be influencedby the persistence of heel striking in mature runners. Olderexperienced runners may have limited ability to transition from heelto forefoot striking when first introduced to barefoot running. Maturerunners should be cautious when beginning a minimalist shoe orbarefoot running regimen.
Return to Sport after Surgical Treatment of Lisfranc Injuries in Athletes: A Retrospective Case Series
Authors: Vopat Bryan G. , Vopat Matthew L. , Van Dijk Pim A.D. , Hazzard Sean , McKinnon Kayla , Asnis Peter D. , Theodore George H. 
Published: June 2020

Introduction
A Lisfranc injury can be a devastating injury in athletes,and if inadequately treated, may lead to chronic pain and lossof function. The purpose of this study was to determine the rate andtime until return to sport after surgical fixation for a ligamentous Lisfrancinjury. We hypothesized that open reduction and screw fixationof a ligamentous Lisfranc injury can be a successful treatment in theathletic population and allow patients to return to sport at close totheir preinjury level of play.
Methods
All patients who were analyzed underwent repair of aligamentous Lisfranc injury with open reduction and screw fixationby a single surgeon, were between 18 - 40 years old at time of theirfinal follow up, and were identified as being an athlete (either recreationalor competitive). Eligible patients were given a questionnairethat included if they were able to return to sport, time until return tosport, subjective percentage of pre-injury level of play, current pain(0 - 10), and complications.
Results
Eleven patients were identified as athletes. Ten (91%) wereavailable for follow-up with a mean of 36.5 months (range, 14 - 60).The average age was 25.4 years (range, 15 - 37) at time of surgery.Eighty percent (8/10) were able to return to sport. The average timeuntil return to sport was 29.4 weeks (range, 22 - 52) with an averagesubjective value of their pre-injury level of play of 87% (range, 70 -100%). However, 67% (6/9) of the athletes had occasional pain withsport with an average pain level of 2.1 (range, 0 - 5). Two patientshad complications, a superficial infection and a deep vein thrombosis.
Conclusion
Most athletes were able to return to sport after undergoingopen reduction and internal fixation of a ligamentous Lisfrancinjury by less than 30 weeks post-surgery with a subjective value of87% of their previous function. However, the majority of the patientsalso experienced some residual pain with their respective sport.These findings suggested that athletes with a ligamentous Lisfrancinjury can have reliably good outcomes with operative repair.
Effect of the dynamic curve type from the perifocal infiltration zone obtained by magnetic resonance imaging on prognosis in pelvic pathologies in women
Authors: Bergen T. A. , Fokin V. A. , Trufanov G. E. , Smagina A. V. , Soynov I. A. 
Published: June 2020

The aim of the study is to evaluate the role and prognostic significance of magnetic resonance imaging (MRI) perfusion methods in prognosis of disease course and outcome based on evaluation of the perifocal infiltration zone in pelvic disorders in women.Materials and methods. Retrospective analysis of pelvic MRI data obtained using MRI system with induced magnetic field of 1.5 T was performed. The study included 530 protocols of pelvic scans in women. Two equal groups (n = 265) of patients with oncological and nononcological pathologies of pelvic organs were formed; after application of propensity score matching, each group contained 165 patients. All examination protocols included dynamic contrast enhancement. The obtained data were statistically analyzed using the Stata 13 software.Results. In the study, types of dynamic curves obtained using dynamic contrast enhancement from the perifocal infiltration zone were analyzed. There was no data showing a correlation between the type of dynamic curve from the zone of perifocal changes and disease prognosis or possibility of differential diagnosis.Conclusions. Evaluation of the perifocal infiltration zone per MRI results with intravenous contrast does not affect prognostic accuracy of the method (p >0.05); contrast can be used only for differential diagnosis or evaluation of local advancement of the process.
Characteristics of oncological care in the reality of COVID-19 pandemic: views of a lawyer and a clinician
Authors: Chernus N. Yu. , Kedrova A. G. , Krasilnikov S. E. , Ivaninskiy O. I. 
Published: June 2020

The past 2019 and the beginning of 2020 were remembered as the beginning of the spread of the threatening human civilization of the Coronavirus Disease 2019 infection (COVID-19) caused by the SARS-CoV-2 coronavirus, that threatens human civilization. In connection with the epidemic, which quickly turned into a pandemic, on March 11, 2020, the World Health Organization declared a public health emergency of international significance, with high risks at the global level. Despite the growing incidence and spread of COVID-19 in our country, ensuring the protection of patients» rights is still one of the priorities of public health. It is necessary to assess the extent to which the re-profiling or introduction of an anti-epidemic regime in medical institutions that provide cancer care restricts the rights of patients whose guarantees, despite the pandemic, are enshrined in current legislation.The aim of the study is to analyze the legislation on restricting the rights of patients in connection with the threat of spread of COVID-19, evaluate the effectiveness and validity of re-profiling and switching to an anti-epidemic mode of operation of medical institutions that provide cancer care.It is noted that any legal prohibitions and restrictions must be justified by the purposes for which they are established, be scientifically justified and not violate the human rights and freedoms proclaimed by the Constitution of the Russian Federation. Measures taken by the state to contain the spread of the COVID-19 epidemic should be necessary to prevent or eliminate possible harm, if this cannot be done by other means and if the harm to the rights of citizens is the least possible.
VARICELA ZOSTER
Authors: Navarrete Cuadrado Katlin Susana , Mastrodomenico Vargas Maleivy , Guao Salinas Hendry , Ruiz Pérez Ornella 
Published: June 2020

La varicela es una enfermedad infecto-contagiosa, producida por el virus varicela-zoster (VVZ); es un virus alfaherpes que se relaciona más estrechamente con el virus del herpes simple, se presenta cuando el virus entra en contacto con la mucosa del tracto respiratorio superior o la conjuntiva de una persona susceptible, la transmisión de persona a persona se puede presentar por contacto directo con lesiones de VZV o por propagación en el aire. La reinfección con VZV es rara. El período de incubación es de 10 a 21 días; los síntomas que generalmente se presentan son fiebre, malestar general, anorexia, dolor de cabeza y posteriormente vesículas claras, pruriginosas en las primeras etapas, llenas de líquido que evolucionan a costras. La complicación más común de la varicela es la sobreinfección bacteriana de las lesiones cutáneas causadas con mayor frecuencia por Staphylococcus aureus y Streptococcus del grupo A, pero también es posible encontrar linfadenitis y abscesos subcutáneos. Cuando la varicela se presenta durante los últimos días de gestación (<5 días) o 2 días después del parto, existe un riesgo aproximado de 20% de presentar varicela neonatal.
IDENTIFY METHODS OF TEACHING AND LEARNING TO CREATE INTEREST, SELF-STUDY, AND CREATIVITY OF STUDENTS
Authors: Hong Van Vu 
Published: June 2020

Purpose: This research indicates the need to identify the right teaching and learning methods, ways to design teaching and learning methods for lecturers, and students in Vietnam's higher education system. From there, point out the limitations of current teaching methods and the advantages when teachers and learners design their new teaching and learning methods.
Methodology: This research uses qualitative and quantitative research methods through interviews about teaching methods of lecturers and student learning methods; evaluation of lecturers and students about the advantages and disadvantages of the current teaching and learning method. Respondents included 1.000 students and 130 lecturers at several universities in the Ho Chi Minh City of Vietnam.
Main Finding: Based on these data and analysis, this study shown a new paradigm in developing active teaching and learning methods for lecturers and students, optimal methods for achieving the expectations that teaching strategies and active learning by the instructors and students.
Implications/Applications: This study contributes to the development of positive teaching and learning strategies, creating interest, and promoting students' self-study ability. The results will help lecturers, and students build proper teaching and learning strategies, creating funny, development capacity, and creativity of students.
Novelty/Originality: The research, analyze and design the essential contents of the strategy development process for both teachers (lecturers) and learners (students). The main contributions of the research are the initial conditions necessary for teachers and students to develop effective teaching and learning strategies for themselves.
CORONAVIRUS MEDIA DISCOURSE AND CURRENT SITUATION (QCA FOR DIFFERENT RESPONSES TO COMBAT COVID-19)
Authors: Singer Nermeen 
Published: June 2020

Purpose of the study: Given the immense spread of the Coronavirus disease, it is imperative to note how the leaders and governments approach the issue and the suggestions made to protect the people and not spread panic.
Methodology: The study incorporates scrutiny of the available online media with official statements and news outlets made by state officials and leaders. Thus, one can note the implementation of qualitative comparative analysis to determine the difference in the attitudes and approaches of various world leaders to combat the disease.
Main Findings: Most world leaders acknowledge the threat Coronavirus poses to humanity yet are willing to sacrifice economic development for the survival of their people. Whereas others are more inclined to disregard the seriousness of the threat despite evidence of the lethal nature of the coronavirus and its effect upon people of all ages.
Applications of this study: The application of the study is primarily in the assessment of the position of world leaders with regards to the assessment of their leadership qualities and the success or failure of their decisions in terms of fighting the onset of the disease. When humanity overcomes coronavirus, numerous world leaders are likely to lose their job with this utilized information being the reason.
Novelty/Originality of this study: Considering the recent outbreak of the pandemic, no-one has utilized nor compared any of the information in terms of how the world leaders and various countries are combating the disease. This study provides a comparative analysis bringing successful and openly failed decisions to the fore of the society allowing every individual to assess the scope and effort made by the state.
INVESTIGATION ON THE URBAN AND RURAL STUDENTS’ BEHAVIOR FOR PLASTIC WASTE MANAGEMENT IN SOLO REGION
Authors: Partono Bambang , Karsidi Ravik , Yusuf Munawir , Sutarno  
Published: June 2020

Purpose of the study: The purpose of this study is to find out the involvement and behavior of students attending school in urban and rural areas in the management of plastic waste. Indicators of student behavior in waste management consist of the types of waste disposed of, students' willingness to dispose of plastic waste according to their grouping, as well as frequent disposal of plastic waste.
Methodology: The research instrument used was a questionnaire and interview conducted in one school in urban areas and one school in rural areas. Subjects involved in the study were high school students in urban and rural areas of Solo.
Main Findings: The results showed that there were enthusiastic differences between rural and urban students in the management of plastic waste; the common concern of urban students was 85%, while rural students were 73%. The research findings show that students who study in urban areas have higher plastic waste care behavior than students who study in rural areas.
Applications of this study: The implications of the results of this study can be useful for further research, namely on the management and use of waste as recycling for both rural and urban students.
Novelty/Originality of this study: There is no investigation on waste management, in both rural and urban schools. Considering waste is one of the main problems in the world today, so the results of investigations can be useful in reducing waste through education.