bluuebunny/crossref_metadata_2025_split huggingface dataset:
5-hydroxymethylcytosine but not MTAP methylation status can stratify malignant pleural mesothelioma based on the lineage of origin
Authors: Bosio Matteo , Salvaterra Elena , Datturi Francesca , Morbini Patrizia , Zorzetto Michele , Inghilleri Simona , Tomaselli Stefano , Mangiarotti Patrizia , Meloni Federica , Cerveri Isa , Stella Giulia Maria 
Published: January 2020

Background: Malignant pleural mesothelioma (MPM) is an aggressive tumor with poor prognosis, mainly associated with work or environmental exposure to asbestos. MPM’s molecular profile is largerly unexplored and effective therapies are still lacking. MPM rarely harbours those somatic genetic lesions that usually characterize solid epithelial-derived tumors. On this basis, our study aims at investigating MPM epigenetic profile. Methods: We here assessed through immunohistochemistry, FISH and methylation specific PCR, the expression of 5-hydroxymethylcytosine (5- hmC) - an epigenetic marker and an important regulator of embryonic development and carcinogenesis - and the methylation status of the promoter of the MTAP gene - encoding for an enzyme involved in the rescue process of methionine and adenine - in two relevant series of FF-PE MPM samples derived from MPM thoracoscopic biopsies. Tissue sampling was performed at diagnosis. Results: Within the limitations of the study cohort, the 5-hmC immunophenotype was different among the histological MPM types analysed. In fact, 18% of the epithelial MPMs were negative, 47% weakly positive, and 35% of the cases showed an intense expression of 5-hmC. Sarcomatoid and biphasic MPMs showed intense 5-hmC expression pattern (positive and weakly positive in more than 80% of cases). Among MPM featuring epithelial lineage, none showed methylation of MTAP promoter. Conclusions: Mesothelial sarcomatoid tumors featured a methylation profile characterized by a permanent gene silencing. Epithelial MPM methylation profile was in-between that of sarcomatoid MPM and the one of epithelial-derived tumors. MTAP promoter methylation level cannot be considered a suitable biomarker of epithelial MPM arousal.
Home exercise improves the quality of sleep and daytime sleepiness of elderlies: a randomized controlled trial
Authors: Sá Brandão Glauber , Sá Brandão Freitas Gomes Glaucia , Sá Brandão Glaudson , Callou Sampaio Antônia A. , Donner Claudio F. , Oliveira Luis V.F. , Camelier Aquiles Assunção 
Published: January 2020

Background: Aging causes physiological changes which affect the quality of sleep. Supervised physical exercise is an important therapeutic resource to improve the sleep of the elderlies, however there is a low adherence to those type of programs, so it is necessary to implement an exercise program which is feasible and effective. The study aimed to test the hypothesis that a semi-supervised home exercise program, improves sleep quality and daytime sleepiness of elderlies of the community who present poor sleep quality.
Methods: This was a randomized controlled trial study, conducted from May to September 2017, in Northeastern Brazil, with elderlies of the community aging 60 years old or older, sedentary, with lower scores or equal to 5 at the Pittsburgh Sleep Quality Index (PSQI) and without cognitive decline. From one hundred ninety-one potential participants twenty-eight refused to participate, therefore, one hundred thirty-one (mean age 68 ± 7 years), and 88% female, were randomly assigned to an intervention group - IG (home exercise and sleep hygiene, n = 65) and a control group - CG (sleep hygiene only, n = 66). Sleep assessment tools were used: PSQI, Epworth sleepiness scaleA (ESS) and clinical questionnaire of Berlin. The level of physical activity has been assessed by means of International Physical Activity Questionnaire adapted for the elderly (IPAQ) and Mini-Mental State Examination for cognitive decline. All participants were assessed before and after the 12-week intervention period and, also, the assessors were blind.
Results: The IG showed significant improvement in quality of sleep with a mean reduction of 4.9 ± 2.7 points in the overall PSQI (p < 0.01) and in all its 7 components of evaluation (p < 0.05), and improvement of secondary endpoint, daytime sleepiness, a decline of 2.8 ± 2.2 points in the ESS (p < 0.01).
Conclusion: Our results suggest that semi-supervised home exercise is effective in improving the quality of sleep and self-referred daytime sleepiness of sedentary elderlies of the community who presented sleep disorders.
Predictors of future microalbuminuria in children and adolescents with type 1 diabetes mellitus in Egypt
Authors: El Habashy Safinaz , Adly Amira , Kader Mohamed , Ali Sherine 
Published: January 2020

IntroductionThe present study was designed to assess the validity and efficacy of urinary markers (NAG, RBP, transferrin, α1-microglobulin, and plasma homocysteine) as early predictors of microalbuminuria in diabetic nephropathy in children and adolescents with type-1 diabetes, and its relation with haemoglobin glycated (HbA<sub>1c</sub>), serum lipid profile, and blood pressure.Material and methodsThis study is a follow-up study to the 2002 study by Salem et al. The present study included 35 type 1 diabetes mellitus (T1DM) children and adolescents recruited from regular attendees of the specialised Diabetology Clinic, Children’s hospital, Ain Shams University, with previously measured urinary N-acetyl-β-glucosaminidase (13) or homocysteine (11) or transferrin (28) or α1-microglobulin (27) or retinol binding protein (13) as an early predictor of diabetic nephropathy in T1DM. Thirty-five patients with type 1 diabetes mellitus were enrolled, and 24 patients were normoalbuminuric at baseline. The patients were tested for markers other than urinary microalbumin, to predict diabetic nephropathy and early renal impairment in children and adolescents with type 1 diabetes mellitus.ResultsRegarding the metabolic control between the studied groups, we found that there is significant difference in HbA<sub>1c</sub> between the microalbuminuric patients and the normoalbuminuric patients. According to the number of positive markers of diabetic nephropathy, the only parameter that was higher in patients with more than one elevated marker was mean systolic blood pressure. Although mean diabetic blood pressure was higher, it was not statistically significant. Regarding to the predictability of urinary markers, urinary N-acetyl-β-glucosaminidase is the most predictable marker with high sensitivity and specificity. The least sensitivity noticed was urinary RBP and the least specificity noticed was urinary α1-microglobulin.ConclusionsRegarding the predictability of urinary markers, urinary NAG is the most predictable marker with both high sensitivity and specificity, with a sensitivity of 60%, specificity 75%, positive predictive value 60%, negative predictive value 75%, and a diagnostic accuracy of 0.58%. Urinary RBP is another marker with low sensitivity but high specificity. Urinary α1-microglobulin is a valid marker with high sensitivity but low specificity. Contrary to previous markers, plasma homocysteine has high specificity but low sensitivity.
MiR-29b-3p affects growth and biological functions of human extravillous trophoblast cells by regulating bradykinin B2 receptor
Authors: Wang Likui , Li Yunguang 
Published: January 2020

IntroductionThis study investigated miR-29b-3p’s effects and mechanisms in preeclampsia development.Material and methodsIn this study, we analysed the pathology and expression of miR-29b-3p and B2R mRNA from normal and preeclampsia placenta tissues using hematoxylin and eosin staining and RT-qPCR assay. For cell experiments, we used transwell assay CCK-8, flow cytometry and wound healing assay to determine the effects and correlation of miR-29b-3p and B2R in HTR-8/SVneo cell proliferation, apoptosis, cell cycle, cell invasion and migration in a preeclampsia cell model. Moreover, the mechanisms were determined using Western blot or immunofluorescence in different groups.ResultsClinical analysis revealed that miR-29b-3p gene expression dramatically increased with increasing degree of preeclampsia (p < 0.001 or p < 0.05, respectively). The HTR-8/SVneo cell biological activities of the model group were significantly depressed (p < 0.001). However, with miR-29b-3p inhibitor or B2R transfection, the HTR-8/SVneo cell biological activities significantly recovered (p < 0.001). Western blot assay showed that B2R, VEGF-A, CCND-1, MMP-2 and MMP-9 levels were suppressed in the model group, compared with those in the NC groups (p < 0.001, respectively). With miR-29b-3p inhibitor or B2R transfection, the protein expression levels of B2R, VEGF-A, CCND-1, MMP-2 and MMP-9 dramatically increased (p < 0.001, respectively).ConclusionsThe down-regulation of miR-29b-3p could improve HTR-8/SVneo cell biological activities in a preeclampsia cell model by targeting B2R.
The role of personality and love style in marital satisfaction: Does similarity matter?
Authors: Odilavadze Mariam , Panjikidze Mariam , Martskvishvili Khatuna , Mestvirishvili Maia , Kvitsiani Mariam 
Published: January 2020

BackgroundScientific study of marital satisfaction attracted widespread attention decades ago. Since then, hundreds of studies have been conducted on determinants of marital satisfaction. The present study attempted to extend previous research on marital life by discussing two important correlates of marital satisfaction: personality traits and love styles. By emphasizing the similarity of personality traits and attitudes toward love in dyads, the study seeks the possible influential constituents for marital outcomes.Participants and procedureEighty-seven (N = 174) married heterosexual couples recruited through a convenience sampling procedure participated in the study. They completed the following questionnaires: the HEXACO Personality Inventory, Love Attitudes Scale, and Revised Dyadic Adjustment Scale (RDAS).ResultsHusbands’ honesty/humility level was positively correlated with their own and wives’ marital satisfaction. Spouses were similar in some love styles. The discrepancy in their attitudes toward love may have negative as well as positive outcomes, depending on whether we consider husbands’ or wives’ marital satisfaction. Personality traits and love styles discrepancy scores predicted participants’ marital satisfaction.ConclusionsPersonality traits and love styles play a significant role in marital satisfaction for both women and men. Marital satisfaction has somewhat different correlates in the case of wives and husbands. Having a different personali-ty or love styles also has different associations with the marital satisfaction of spouses.
Why we put on the sorting hat: motivations to take fan personality tests
Authors: Proudfoot Steven , Plante Courtney , Reysen Stephen 
Published: January 2020

BackgroundThere is little reason to believe that fan-related personality tests, which tell fans what type of person they are based on their fa-vorite fan content, are valid or reliable. Nevertheless, fan-related personality tests remain incredibly popular online.Participants and procedureBuilding upon existing fan research, the present study tests whether fans may have other motivations for taking such personality tests, drawing upon prior research by Wann. Self-identified fans (N = 425, Mage = 26.41, SD = 8.44) completed measures re-garding degree of identification with their fan interest, motivations to take fan quizzes, and frequency of taking fan personality quizzes.ResultsHighly-identified fans were found to more frequently take fan-related personality tests, an association significantly mediated by both self-esteem and escapism motivations, but not mediated by eustress, entertainment, belongingness, or family motivations.ConclusionsThe results suggest that highly identified fans participate in more fan personality quizzes to escape from the hassles of everyday life and increase positive self-worth. These results are discussed with respect to their theoretical relevance – both for research on personality testing and on fan activities – as well as for their practical implications.
Clinical features and outcome of mixed connective tissue disease in developmental age – observational study from one center
Authors: Rutkowska-Sak Lidia , Gietka Piotr 
Published: January 2020

ObjectivesMixed connective tissue disease is a rare systemic connective tissue disease of developmental age and it includes the features of arthritis, polymyositis/dermatomyositis, systemic lupus erythematosus and systemic sclerosis, with presence of anti-ribonucleoprotein antibodies (anti-RNP) in serum. Early diagnosis of the disease is difficult but essential in preventing development of systemic complications, which are often irreversible. International literature does not report many studies on large cohorts of children with this disease. The aim of this retrospective study was to define clinical characteristics and long-term results of treatment of the disease in 60 children with mixed connective tissue disease hospitalized in the period between 1978 and 2018. The diagnosis was established on the basis of Kasukawa’s criteria.Material and methodsIt was a group of 60 children (46 girls and 16 boys) aged 10.5 on average (4–16.5). When assessing general symptoms at the onset of the disease according to Kasukawa’s criteria, the highest number, over 80% of children, demonstrated symptoms suggesting SLE, about 40% suggesting DM and about 25% suggesting SSC. In the period of observation the number of children with clinical symptoms suggesting SSC increased. The most common clinical symptoms included Raynaud syndrome, arthritis and myositis and the most common irregularities in the test results included presence of anti-RNP antibodies and rheumatoid factor and hematological symptoms such as leukopenia/thrombocytopenia. Restrictive lung function impairment was demonstrated by 20% of children. Treatment most often included combined therapy (glucocorticosteroids + methotrexate/azathioprine).ResultsIn 70% of the patients stable improvement was observed. Remission concerned 7% of the patients, frequent exacerbations were found in almost 20% of patients, and 2 children (3.5%) died.ConclusionsThe long term observations of patients in developementeal age with mixed connective tissue disease revealed that the majority of them had domination of SLE symptoms, only in 7% achieved remission and 70% remained in stable improvement. Serious infections with septic state were the cause of death in two cases.
Acute Achilles tendon rupture after levofloxacin in a patient with giant cell arteritis
Authors: Stasiek Małgorzata , Głuszko Piotr 
Published: January 2020

The authors report a case of a 67-year-old woman with giant cell arteritis with acute Achilles tendon rupture, which occurred after 3 days of levofloxacin therapy introduced because of newly diagnosed erosive gastritis associated with Helicobacter pylori infection. The Achilles tendon rupture was surgically treated and the patient made a complete recovery. In view of the widespread use of levofloxacin in practice, this case report raises important clinical implications. Tendinopathies are a known complication, quite rare in the healthy population, but the risk of rupture significantly increases in the population of patients over 60 years of age, with chronic usage of glucocorticosteroids, impaired renal function and recipients of organ transplants. What needs underlining, there are also described differences between individual fluoroquinolones as a cause of tendon damage in this group. <br />
Considering the widespread use of this group of drugs in patients, knowledge about the risk of adverse events including tendinopathy promotes safe use of fluoroquinolones.
Monogenic autoinflammatory diseases in adults – a challenge to rheumatologic practice at the onset of the Polish national programme of interleukin 1 inhibitor treatment
Authors: Milchert Marcin , Makowska Joanna , Brzezińska Olga , Brzosko Marek , Więsik-Szewczyk Ewa 
Published: January 2020

Monogenic autoinflammatory diseases (AIDs, formerly known as hereditary periodic fever syndromes) cover a spectrum of diseases which lead to chronic or recurrent inflammation caused by activation of the innate immune system. The most common monogenic AID is familial Mediterranean fever. Monogenic autoinflammatory diseases are generally considered intracellular signalling defects. Some stereotypical knowledge may be misleading; e.g. monogenic AIDs are not exclusively found in children, family history is often negative, fever frequently is not a leading manifestation and frequency of attacks in adults is usually variable. Lack of genetic confirmation should not stop anti-inflammatory ex juvantibus therapy. The pattern of tissue injury in AIDs is basically different from that observed in autoimmunity. There is no autoaggression against organ-specific antigens, but substantial damage (amyloidosis, cachexia, premature cardiovascular disease) is secondary to long-lasting inflammation.<br />
The Polish national programme of anti-interleukin 1 treatment opens new possibilities for the treatment. However, monogenic AIDs are frequently misdiagnosed and more awareness is needed.
Stream temperature and discharge evolution in Switzerland over the last 50 years: annual and seasonal behaviour
Authors: Michel Adrien , Brauchli Tristan , Lehning Michael , Schaefli Bettina , Huwald Hendrik 
Published: January 2020

Abstract. Stream temperature and discharge are key hydrological variables for ecosystem and water resource management and are particularly sensitive to climate warming. Despite the wealth of meteorological and hydrological data, few studies have quantified observed stream temperature trends in the Alps. This study presents a detailed analysis of stream temperature and discharge in 52 catchments in Switzerland, a country covering a wide range of alpine and lowland hydrological regimes. The influence of discharge, precipitation, air temperature, and upstream lakes on stream temperatures and their temporal trends is analysed from multi-decadal to seasonal timescales. Stream temperature has significantly increased over the past 5 decades, with positive trends for all four seasons. The mean trends for the last 20 years are +0.37±0.11 ∘C per decade for water temperature, resulting from the joint effects of trends in air temperature (+0.39±0.14 ∘C per decade), discharge (-10.1±4.6 % per decade), and precipitation (-9.3±3.4 % per decade). For a longer time period (1979–2018), the trends are +0.33±0.03 ∘C per decade for water temperature, +0.46±0.03°C per decade for air temperature, -3.0±0.5 % per decade for discharge, and -1.3±0.5 % per decade for precipitation. Furthermore, we show that snow and glacier melt compensates for air temperature warming trends in a transient way in alpine streams. Lakes, on the contrary, have a strengthening effect on downstream water temperature trends at all elevations. Moreover, the identified stream temperature trends are shown to have critical impacts on ecological and economical temperature thresholds (the spread of fish diseases and the usage of water for industrial cooling), especially in lowland rivers, suggesting that these waterways are becoming more vulnerable to the increasing air temperature forcing. Resilient alpine rivers are expected to become more vulnerable to warming in the near future due to the expected reductions in snow- and glacier-melt inputs. A detailed mathematical framework along with the necessary source code are provided with this paper.
A A deriva e a psicogeografia e suas possibilidades para os trabalhos de campo em Geografia Urbana
Authors: Gonçalves Glauco Roberto 
Published: January 2020

Deriva e psicogeografia são propostas prático-teóricas elaboradas pela Internacional Situacionista com o intuito de desvendar criticamente o cotidiano atrelado ao processo de urbanização. Este artigo oferece um percurso constitutivo destas propostas ao longo da história da Internacional Situacionista e tenta estabelecer as relações e razões pelas quais a deriva e a psicogeografia constituem ferramentas de grande valia para os trabalhos de campo em Geografia Urbana.
Palavras-chave: psicogeografia; deriva; Internacional Situacionista; trabalho de campo em Geografia Urbana; urbano e vida cotidiana.
Índice de pobreza humana municipal para Mato Grosso do Sul
Authors: Barbosa Francis Régis Gonçalves Mendes , Balsalobre Cristiane de Matos , Sonaglio Claudia Maria 
Published: January 2020

A identificação dos fatores que causam a pobreza multidimensional é imprescindível para a obtenção de uma visão apurada dos problemas enfrentados pela sociedade decorrentes desse problema. Nesse sentido, o objetivo geral foi mensurar a pobreza multidimensional e analisar a sua evolução para os municípios de Mato Grosso do Sul nos anos de 1991, 2000 e 2010. Foi criado o Índice de Pobreza Humana Municipal (IPHM) a partir das variáveis de saúde, educação e acesso a serviços básicos domiciliares. Os resultados apontam que os municípios de Japorã, Tacuru, Paranhos e Juti permanecem entre os mais pobres no período estudado. Os municípios de Campo Grande, Chapadão do Sul, São Gabriel do Oeste e Dourados permanecem entre os menos pobres. Conclui-se que houve melhoria no bem-estar social dos munícipes, como consequência de políticas públicas adotadas para o desenvolvimento e qualidade de vida da população. ABSTRACT The identification of the factors that cause the multidimensional poverty is essential for obtaining an accurate view of the problems faced by society arising from this reality. In this sense, the main objective was to measure the multidimensional poverty and to analyze its evolution for the municipalities of Mato Grosso do Sul in the years 1991, 2000 and 2010. The Municipal Human Poverty Index (MHPI) was created based on health, education and access to basic household services variables. The results indicate that the municipalities of Japorã, Tacuru, Paranhos and Juti remain among the poorest in the studied period. The municipalities of Campo Grande, Chapadão do Sul, São Gabriel do Oeste and Dourados remain among the poorest. It was concluded that there was an improvement in the social well-being of the counties, as consequence of public policies adopted for the development and quality of life of the population. Keywords: Multidimensional Poverty; Poverty Index; Regional Disparities.
Pathogen Inducible cis-acting Elements of Synthetic Promoters in Plants-review
Authors: Hegde Rashmi 
Published: January 2020

Synthetic pathogen inducible promoters are used for the improvement and application of transgenic techniques in research and to increase agriculture production. The promoter contains specific cis-regulatory elements (W box, GCC box, Box S and D box) which induce anti pathogen molecular cascades. Insertion of dimerized form of cis acting elements at upstream region of promoter in promoter probe vector drives the expression of resistance gene or reporter gene. The expression indicates that synthetic promoters are responded to fungal elicitors. Expression of resistance restrict to infection sites which boost disease resistance in plants.