bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sustainable Agricultural Growth for Rural Development in Asia: A Review*
Authors: Choi Kyung Sook , Labhsetwar Vijay K. 
Published: August 2020

AbstractThe current world population of 7.6 billion is expected to reach 8.6 billion in 2030 and 9.8 billion in 2050. Most of the addition will be in developing countries, and the exponential growth in population would require doubling the current food production.This paper discusses several strategies developed to cope with sustainable agricultural growth for rural development in Asia. In Korea, despite the decline in investment in food production in recent times, continuous interventions from the government are critical because agricultural water use is essential for the improvement of rural living standards. Thailand shifted its rural development from a growth‐oriented approach to a new model of holistic people‐centred and sustainable development. Japan emphasized the importance of multi‐functionality to be taken into account to evaluate water use for agriculture in Asia. Indonesia accepted challenges based on the current roles of irrigation development in food security and poverty eradication. Turkey flagged its group agricultural practices (GAPs), a south‐eastern Anatolia project, to showcase its multi‐sector and integrated regional development approach in the context of sustainable development.To cope with food insecurity, it is necessary to find effective ways to use agricultural water for production and environmental services at the same time for sustainable rural development.
MiR‐134‐5p targeting XIAP modulates oxidative stress and apoptosis in cardiomyocytes under hypoxia/reperfusion‐induced injury
Authors: Lu Min , Qin Xinglei , Yao Jungong , Yang Yuanyuan , Zhao Minghu , Sun Lin 
Published: August 2020

AbstractMicroRNA‐134‐5p (MiR‐134‐5p) has been proposed as a promising novel biomarker for the diagnosis of acute myocardial infarction (AMI). However, the biological role of miR‐134‐5p in ischemic cardiomyocytes has been little disclosed yet. Expression of miR‐134‐5p and X‐linked inhibitor of apoptosis protein (XIAP) was detected using RT‐qPCR and western blot. Oxidative stress and cell apoptosis were determined by enzyme‐linked immunosorbent assays, 3‐(4, 5‐dimethylthiazole‐2‐y1)‐2, 5‐biphenyl tetrazolium bromide assay, flow cytometry, western blot, and terminal‐deoxynucleoitidyl transferase‐mediated nick end labeling (TUNEL). The interaction between miR‐134‐5p and XIAP was confirmed by luciferase reporter assay. Expression of miR‐134‐5p was upregulated in serum of AMI patients and hypoxia/reoxygenation (H/R)‐induced cardiomyocytes (AC16 and HCM). MiR‐134‐5p downregulation could inhibit H/R‐mediated release of lactic dehydrogenase enzyme (LDH) and malondialdehyde (MDA), and promote superoxide dismutase (SOD) and glutathione peroxidase (GSH‐PX) levels. Meanwhile, cell viability was increased, while the apoptosis rate and TUNEL positive cells were inhibited by miR‐134‐5p downregulation in H/R‐treated AC16 and HCM cells. Mechanically, XIAP was downregulated and targeted by miR‐134‐5p in H/R‐induced cardiomyocytes in vitro. Overexpression of XIAP inhibited oxidative stress and cell apoptosis in H/R‐treated AC16 and HCM cells, which was similar to miR‐134‐5p knockdown. Moreover, downregulation of XIAP could partially reverse the effect of miR‐134‐5p knockdown in H/R‐induced cardiomyocytes. Knockdown of miR‐134‐5p protected cardiomyocytes from H/R‐induced oxidative stress and apoptosis in vitro through targeting XIAP.
Role of prophylactic and therapeutic red blood cell exchange in pregnancy with sickle cell disease: Maternal and perinatal outcomes
Authors: Yılmaz Baran Şafak , Kozanoğlu İlknur , Korur Aslı , Doğan Durdağ Gülşen , Kalaycı Hakan , Alemdaroğlu Songül , Asma Süheyl , Kılıçdağ Esra Bulgan , Boğa Can 
Published: August 2020

AbstractBackground and AimThe incidence of fetomaternal complications during pregnancy is high for women with sickle cell disease (SCD), which is the most common hematologic genetic disorder worldwide. Prophylactic red blood cell exchange (pRBCX) has been shown to be efficient, safe, and feasible for preventing complications. The aim of this study was to observe maternal, perinatal, and neonatal outcomes of pregnancies in which pRBCX was.MethodThis was a single‐center, retrospective, cross‐sectional study, which recruited 46 consecutive adult pregnant women with SCD between January 2012 and June 2019. Obstetric features, SCD‐related complications, and fetomaternal outcomes were compared between the 27 patients who received prophylactic exchange and the 19 who did not (therapeutic exchange was performed in 7 and was not performed in 12 cases).ResultsPainful crises, preeclampsia, and preterm birth rates were significantly higher in the group that did not receive prophylactic exchange (control group; P = .001, P = .024, and P = .027, respectively). There was one maternal mortality in the control group (P = .41). Incidence of adverse fetal or maternal complications was significantly higher in the control group (P = .044 and P = .007, respectively).ConclusionsOur center's experience over a 7.5‐year period, as described above, demonstrates that pRBCX in SCD affects the course of pregnancy positively by ameliorating negative fetomaternal outcomes.
HOXB‐AS1 accelerates the tumorigenesis of glioblastoma via modulation of HOBX2 and HOBX3 at transcriptional and posttranscriptional levels
Authors: Bi Yongyan , Mao Yuhang , Su Zuopeng , Du Jiarui , Ye Liping , Xu Fulin 
Published: August 2020

AbstractGlioblastoma (GBM) is the most universal and invasive brain tumor among adults. Increasing studies have reported that long noncoding RNAs play vital roles in regulating downstream molecules at the transcriptional or posttranscriptional level in tumor progression. The purpose of the current research was to inquire the modulation mechanism by which homeobox B cluster antisense RNA 1 (HOXB‐AS1) functioned in GBM. Our study first discovered the lifted expression of HOXB‐AS1 and its nearby genes HOXB2 and HOXB3 in GBM and the positive relationship between HOXB‐AS1 and HOXB2 or HOXB3. Loss‐of‐function assays and in vivo study detected that silencing of HOXB‐AS1, HOXB2, or HOXB3 restrained the proliferation and induced the apoptosis in GBM. In addition, mechanism experiments demonstrated that HOXB‐AS1 recruited interleukin enhancer‐binding factor 3 (ILF3) to regulate HOXB2 and HOXB3 expression at the transcriptional level, and HOXB‐AS1 sponged miR‐186‐5p to modulate HOXB2 and HOXB3 expression at posttranscriptional level. Finally, the regulatory mechanism of HOXB‐AS1 in GBM was certified through rescue experiments. Our results indicated that HOXB‐AS1 boost the HOXB2 or HOXB3 expression at the transcriptional and posttranscriptional levels. We detected the HOXB‐AS1‐ILF3‐HOXB2/HOXB3 axis and HOXB‐AS1‐miR‐186‐5p‐HOXB2/HOXB3 axis driving the GBM progression, which might generate more effective diagnostic biomarkers and therapeutic targets for patients with GBM.
Sorafenib as second‐line treatment option after failure of lenvatinib in patients with unresectable hepatocellular carcinoma
Authors: Tomonari Tetsu , Sato Yasushi , Tanaka Hironori , Tanaka Takahiro , Taniguchi Tatsuya , Sogabe Msasahiro , Okamoto Koichi , Miyamoto Hiroshi , Muguruma Naoki , Takayama Tetsuji 
Published: August 2020

AbstractBackground and AimCurrently, there is no molecular‐targeted agent that has demonstrated evidence of efficacy in patients with unresectable hepatocellular carcinoma (u‐HCC) who have developed resistance to treatment with lenvatinib (LEN). In this real‐world study, we aimed to investigate the therapeutic effect and safety of sorafenib (SOR) in patients with u‐HCC after progression on treatment with LEN.Methods (Patients) and ResultsA total of 13 patients with u‐HCC (12 males and 1 female), who were treated with SOR after progression on LEN, were enrolled in this retrospective study. Therapeutic efficacy was evaluated via contrast‐enhanced computerized tomography at 8 weeks after the initiation of SOR therapy according to modified response evaluation criteria in solid tumors (mRECIST) and RECIST. According to mRECIST, the objective response rate (ORR) and disease control rate (DCR) were 15.3% (2/13) and 69.2% (9/13), respectively. According to RECIST, the ORR and DCR were 0% (0/13) and 69.2% (9/13), respectively. The median progression‐free survival was 4.1 months. The median albumin‐bilirubin scores did not deteriorate significantly at 4, 6, and 8 weeks after initiation of SOR, compared with the scores at the baseline. The most frequent grade 1 or 2 adverse events (AEs) were palmar–plantar erythrodysesthesia, fatigue, diarrhea, and hypertension. There was no incidence of grade 3 AEs.ConclusionTreatment with SOR may be effective for u‐HCC after failure on LEN and may not worsen the liver reserve.
Ionic liquid promoted green synthesis of new pyridazino benzazepine derivatives: Evaluation of antioxidant activity
Authors: Dehbandi Behnam , Hossaini Zinatossadat , Mirjafari Zohreh , Zardoost Mohammad Reza 
Published: August 2020

AbstractIn this research, we investigated preparation of pyridazino benzazepine derivatives in high yields via the domino and one‐pot reaction of isatoic anhydride, N‐methylimidazole, alkyl bromides, activated acetylenic compounds, and hydrazine in ionic liquid as green media at 80°C. Also, antioxidation property of some prepared pyridazino benzazepines due to having pyridazin and benzazepine core is investigated by employing of trapping diphenyl‐picrylhydrazine radical and ability of ferric reduction experiment. Our procedure has a few benefits relative to reported method such as good rate of reaction, product with high efficiency, and simple separation of product from mixture of reaction.
Fabry disease in India: A multicenter study of the clinical and mutation spectrum in 54 patients
Authors: Nampoothiri Sheela , Yesodharan Dhanya , Bhattacherjee Amrita , Ahamed Hisham , Puri Ratna Dua , Gupta Neerja , Kabra Madhulika , Ranganath Prajnya , Bhat Meenakshi , Phadke Shubha , Radha Rama Devi Akella , Jagadeesh Sujatha , Danda Sumita , Sylaja Padmavathy Narayana , Mandal Kausik , Bijarnia‐Mahay Sunita , Makkar Ravinder , Verma Ishwar Chander , Dalal Ashwin , Ramaswami Uma 
Published: August 2020

AbstractFabry disease (FD) is a treatable X linked lysosomal storage disorder with a wide phenotypic spectrum. There is a scarcity of published data on the burden of FD in India. This study evaluates the clinical and molecular spectrum of Indian patients with FD. In this multicentric study involving 10 tertiary referral centers in India, we analyzed the clinical course and genotype of 54 patients from 37 families. Family screening identified 19 new patients (35%) from 12 index cases. Then, 33 GLA gene variants were identified in 49/54 (90.7%) which included 11 novel and 22 known pathogenic variants. Of the 54 patients in our cohort, 40 patients had “classical” and 10 patients had a “nonclassical” presentation. The symptoms and signs included kidney dysfunction in 38/54 (70.3%), neuropathic pain in 34/54 (62.9%), left ventricular hypertrophy in 22/49 (44.8%) and stroke in 5/54 (9.2%). Female heterozygotes were 10/54 (18.5%) of whom 2 were index cases. There was a significant delay in reaching the diagnosis of 11.7 years. Enzyme replacement therapy was initiated in 28/54 (51.8%) patients with significant improvement of neuropathic pain and gastrointestinal symptoms. This study highlights the clinical presentation and mutational spectrum of FD in India and suggests that family screening and screening of high‐risk groups (hypertrophic cardiomyopathy, idiopathic chronic renal failure and cryptogenic stroke) could be the most cost‐effective strategies for early identification of FD.
Novel T2 Mapping for Evaluating Cervical Cancer Features by Providing Quantitative T2 Maps and Synthetic Morphologic Images: A Preliminary Study
Authors: Li Shujian , Liu Jie , Zhang Feifei , Yang Meng , Zhang Zanxia , Liu Jingjing , Zhang Yong , Hilbert Tom , Kober Tobias , Cheng Jingliang , Zhu Jinxia 
Published: August 2020

BackgroundThe application value of T2 mapping in evaluating cervical cancer (CC) features remains unclear.PurposeTo investigate the role of T2 values in evaluating CC classification, grade, and lymphovascular space invasion (LVSI) in comparison to apparent diffusion coefficient (ADC), and to compare synthetic T2‐weighted (T2W) images calculated from T2 values to conventional T2W images for CC staging.Study TypeRetrospective.PopulationSixty‐three patients with histopathologically confirmed CC.Field Strength/Sequence3T, conventional T2W turbo spin‐echo, diffusion‐weighted echo‐planar, and accelerated T2 mapping sequence.AssessmentT2 and ADC values between different pathological features of CC were compared. The diagnostic accuracies of conventional and synthetic T2W images in staging were also compared.Statistical TestsParameters were compared using an independent t‐test, Wilcoxon signed‐rank test, and the chi‐square test. Receiver operating characteristic analysis was performed.ResultsThe T2 values varied significantly between well/moderately differentiated and poorly differentiated tumors ([92.8 ± 9.5 msec] vs. [83.8 ± 9.5 msec], P < 0.05) and between LVSI‐positive and LVSI‐negative CC ([82.2 ± 8.2 msec] vs. [93.9 ± 9.1 msec], P < 0.05). The ADC values showed a significant difference for grade ([0.76 ± 0.10 × 10−3 mm2/s] vs. [0.65 ± 0.11 × 10−3 mm2/s], P < 0.05) and no difference for LVSI status ([0.71 ± 0.11× 10−3 mm2/s] vs. [0.73 ± 0.12× 10−3 mm2/s], P = 0.472). There was no significant difference in T2 and ADC values between squamous cell carcinoma and adenocarcinoma (P = 0.378 and P = 0.661, respectively). In MRI staging, the conventional and synthetic T2W images resulted in a similar accuracy (71% vs. 68%, P = 0.698).Data ConclusionThe accelerated T2 mapping sequence may facilitate grading and staging of CC by providing quantitative T2 maps and synthetic T2W images in one acquisition. T2 values may be superior to ADC in predicting LVSI.Level of Evidence2Technical Efficacy Stage2 J. MAGN. RESON. IMAGING 2020;52:1859–1869.
PET/MRI in Cervical Cancer: Associations Between Imaging Biomarkers and Tumor Stage, Disease Progression, and Overall Survival
Authors: Shih I‐Lun , Yen Rouh‐Fang , Chen Chi‐An , Cheng Wen‐Fang , Chen Bang‐Bin , Chang Yu‐Hsuan , Cheng Mei‐Fang , Shih Tiffany Ting‐Fang 
Published: August 2020

BackgroundPositron emission tomography (PET)/MRI biomarkers have been shown to have prognostic significance in patients with cervical cancer. Their associations with progression‐free survival (PFS) and overall survival (OS) merit further investigation.PurposeTo evaluate the association between PET/MRI biomarkers and tumor stage, PFS, and OS in patients with cervical cancer.Study TypeProspective cohort study.PopulationIn all, 54 patients with newly diagnosed cervical cancer and measurable tumors (>1 cm) were included in the image analysis.Field Strength/Sequence3.0T integrated PET/MRI including diffusion‐weighted echo‐planar imaging (b = 50 and 1000 s/mm2) and [18F]fluorodeoxyglucose PET.AssessmentTwo radiologists measured the minimum and mean apparent diffusion coefficient (ADCmin and ADCmean), maximum standardized uptake value (SUVmax), metabolic tumor volume (MTV), and total lesion glycolysis (TLG) of the primary tumors.Statistical TestsA Mann–Whitney U‐test was used to evaluate the association between the imaging biomarkers and tumor stage. A Cox proportional hazards model was used to assess the relationships between the imaging biomarkers and survival.ResultsIn advanced tumors (T ≥ 1b2, M1, stage ≥ IB3), ADCmin was significantly lower and MTV, TLG, MTV/ADCmin, and TLG/ADCmin were significantly higher (P values between <0.001 and 0.036). In N1 tumors, ADCmin was significantly lower and MTV and MTV/ADCmin were significantly higher (P values between 0.005 and 0.016). In survival analysis, SUVmax was an independent predictor of PFS (hazard ratio [HR] = 4.57, P < 0.05), and ADCmin was an independent predictor of OS (HR = 0.02, P < 0.05). In subgroup analysis of patients with different stages, MTV/ADCmin was a predictor of PFS in stage I disease (P = 0.003), ADCmin (P = 0.038), and MTV (P = 0.020) in stage II, SUVmax (P = 0.006), and TLG (P = 0.006) in stage IV; and ADCmin was a predictor of OS in stage III disease (P = 0.008).Data ConclusionPET/MRI biomarkers of cervical cancer are associated with tumor stage and survival. SUVmax and ADCmin are independent predictors of PFS and OS, respectively.Level of Evidence1Technical Efficacy3
Noncontrast MR Lymphography in Secondary Lower Limb Lymphedema:
Authors: Cellina Michaela , Martinenghi Carlo , Panzeri Marta , Soresina Massimo , Menozzi Andrea , Daniele Gibelli , Oliva Giancarlo 
Published: August 2020

BackgroundInvasive imaging techniques have been applied for lymphedema (LE) assessment; noncontrast MR lymphography (NCMLR) has potential as an alternative, but its performance is not known in secondary lower limb LE.PurposeTo assess the role of NCMRL for the classification and characterization of secondary lower limb LE.Study TypeRetrospective.PopulationFifty adults with clinically diagnosed secondary LE.Field Strength/Sequence1.5T, 3D T2‐weighted turbo spin‐echo, 3D T2‐weighted turbo spin‐echo short tau inversion recovery.AssessmentThree radiologists assessed the following characteristics on NCMRL: honeycomb pattern, dermal thickening, muscular abnormalities, distal dilated lymphatics, inguinal lymph node number, appearance of iliac lymphatic trunks. An LE grading based on the MR images was assigned. The relationship between imaging findings and clinical staging was evaluated, as well as between dermal backflow at lymphoscintigraphy and MR staging, and between the limb swelling duration and peripheral lymphatics dilatation.Statistical TestsPearson's correlation test and Cramer's V coefficient were computed to measure the strength of association. The Mann–Whitney test was used to compare the limb swelling duration between patients with and without dilated distal vessels. Agreement among raters was assessed through Kendall's W coefficient of correlation.ResultsClinical stage and the MR grading were correlated, with Cramer's V coefficient of 1 for reader 1 (P < 0.05), 0.846 for reader 2 (P < 0.05), and 0.912 (P < 0.05) for reader 3; agreement between interraters was very good (W = 0.0.75; P = 0.05). A honeycomb pattern (P < 0.05), dermal thickening (P < 0.001), muscular abnormalities (P < 0.05), iliac lymphatic trunks appearance (P < 0.05), distal dilated vessels (P < 0.05), and lymph nodes number (P < 0.05) were significantly correlated with LE clinical stage. Dermal backflow at lymphoscintigraphy was described in 10 (20%) patients and showed a significant correlation with the MR grading (P < 0.05).Data ConclusionThese preliminary results suggest that NCMRL may provide information useful for the staging and management of patients affected by secondary lower limb LE.Level of Evidence 4Technical Efficacy Stage 2J. MAGN. RESON. IMAGING 2021;53:458–466.
Clinical usefulness of fully automated chemiluminescent immunoassay for quantitative antibody measurements in COVID‐19 patients
Authors: Soleimani Reza , Khourssaji Mehdi , Gruson Damien , Rodriguez‐Villalobos Hector , Berghmans Mathilde , Belkhir Leila , Yombi Jean‐Cyr , Kabamba‐Mukadi Benoît 
Published: August 2020

AbstractSince December 2019, we have been in the battlefield with a new threat to the humanity, known as severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2), which causes coronavirus disease 2019 (COVID‐19), characterized by viral pneumonia. It may be asymptomatic or cause various symptoms, ranging from flu‐like symptoms to acute respiratory distress syndrome and eventually death. At present, the only reliable test for COVID‐19 diagnosis is quantitative reverse transcriptase‐polymerase chain reaction. Assessing the immune response against SARS‐CoV‐2 could increase the detection sensitivity of infected population. Hereby, we report the performances of a fully automated chemiluminescent immunoassay (CLIA) on 276 serum samples. One hundred samples obtained from COVID‐19 negative subjects (COVID‐19 free) were analyzed to evaluate the diagnostic specificity of antibody (Ab) detection. Thereafter, 176 samples obtained from 125 patients with confirmed COVID‐19 (COVID‐19 patients) were selected to assess the diagnostic sensitivity of the CLIA. All samples were analyzed on MAGLUMI 800 platform. All COVID‐19 free samples had Ab levels below the cutoff values. Hence, the diagnostic specificity was estimated at 100% (95% confidence interval [CI] = 96.3‐100.0; positive predictive value = 100%). By the 18th day from the onset of symptoms, we reached an optimal diagnostic sensitivity (more than 95.0%) In fact, the diagnostic sensitivity increased over time and between 15 and 25 days after symptoms onset, reached 95.5% (95% CI = 84.9‐99.2). The new automated CLIA analyzer appeared to be a robust and reliable method to measure specific Ab against COVID‐19 at high throughput. Our data suggest that combining Ab and nucleic acid detection could increase diagnostic sensitivity.
Can Phase Angle in Newborns at Neonatal Intensive Care Units Be an Indicator of Mortality and Prognosis?
Authors: Coradine Andréa Vieira Pereira , Lima Mônica Nunes , Sarquis Ana Lúcia 
Published: August 2020

AbstractBackgroundThe bioelectrical impedance (BI) phase angle (PA), analyzed directly through BI analysis (BIA), is determined by tissue cellularity, representing a direct measure of cellular stability and, for this reason, has been studied and considered as an indicator of prognosis and nutrition status in adults and children.ObjectiveWe aimed to determine if PA can be an indicator of mortality and prognosis in newborns admitted to the neonatal intensive care unit (NICU).MethodsTransversal study conducted at a public NICU in Curitiba, Paraná, Brazil. All newborns, preterm and term, were considered eligible for the study if admission to the NICU occurred by the first hour of life. The Score for Neonatal Acute Physiology II, as well as the Perinatal Extension version, were developed to assess the risk of mortality for all newborns, measured within 12 hours of admission. BIA measurements were conducted using the tetrapolar BioScan Maltron 916, with single‐frequency (50 kHz) tetrapolar BI. PA was calculated as the arc tangent: (Xc/R) x 180°/π.ResultsBIA was measured during the first 24 hours of admission for all newborns (n = 93), repeated between 24 and 48 hours (n = 79) and again after 7 days (n = 55), always when possible. PA measurements decreased in the first 48 hours in premature newborns, particularly among those who died. The premature newborns also showed a significant decrease from the first to the last PA measurement (P = .001). In addition, whereas full‐term newborns showed an increase of PA at 1 week of life, preterm infants continued to have a decrease in values. For preterm newborns, PA measurements decreased and more sharply so for those who died. This result should be viewed with caution given the small number of deaths, but it should be investigated to understand the role of PA in the prognosis of NICU newborns.ConclusionsThe absolute value of PA during the first 24 hours of life was not a good marker for severity or mortality. However, the decrease of PA between different moments of evaluation was a good marker of severity. The decrease of PA in the first 48 hours in premature newborns, and that when the decrease is more pronounced, may be indicative of mortality. The difference in PA values between these newborns is probably a significant variable for mortality and prognosis and not a cutoff value.