bluuebunny/crossref_metadata_2025_split huggingface dataset:
Práticas de educação em saúde dos profissionais de enfermagem para o autocuidado de pacientes com colostomia: scoping review
Authors: Dantas Daniella Canejo , Magalhães Ariadne Gomes da Costa , Ribeiro Yonara Cristiane , Diaz Diana Paola Gutierrez , Xavier Brunno Lessa , Barreto Ana Claudia Mateus 
Published: November 2020

O objetivo deste scoping review é mapear as evidências científicas sobre as práticas de educação em saúde utilizadas pelos profissionais de enfermagem para promover o autocuidado de pacientes com colostomia. Trata-se de uma revisão de escopo pautada nas orientações metodológicas do Joanna Briggs Institute, onde buscou responder à seguinte pergunta de pesquisa: quais as práticas de educação em saúde desenvolvidas e utilizadas pelos profissionais de enfermagem para o autocuidado de pacientes com colostomia? Buscaram-se artigos que abordavam explicitamente os elementos mnemônicos do estudo: População – pacientes colostomizados, Conceito - práticas de educação em saúde dos profissionais de enfermagem para o autocuidado de pacientes com colostomia e Contexto - hospitalar, domiciliar, atenção primária. As estratégias de busca permitiram recuperar 509 fontes de evidências, dos quais 373 foram retirados por corresponder a registros duplicados e mantidos 136 para análise. Por não se enquadrarem nos critérios de inclusão 110 artigos completos foram excluídos, restando 08 estudos a serem incluídos nesta pesquisa. Conclui-se que para dar apoio ao enfermeiro, as tecnologias educacionais se mostram aliadas no intuito de tornar o momento de orientação sobre o autocuidado com as estomias mais fácil e aprazível, minimizando o impacto do grande quantitativo de informações a serem dadas. Conclui-se que os recursos para realizar a educação em saúde estão se adentrando cada vez mais na enfermagem, contribuindo assim com a promoção do autocuidado, agregando valor aos pacientes e proporcionando uma melhora na qualidade de vida daqueles que dependem de uma bolsa coletora.
Internet das Coisas e Educação: uma revisão sistemática da literatura
Authors: Lima Claudio Cleverson de , Schlemmer Eliane , Morgado Leonel 
Published: November 2020

A Internet das Coisas (IoT ou Internet of Things) tem se revelado uma tecnologia potencialmente disruptiva em vários campos da atividade humana, o que inclui os processos educacionais. O processamento de dados produzidos e armazenados por objetos cotidianos e sensores e processados na web pode potencializar a análise pedagógica do professor e conferir maior liberdade para intervir nos percursos formativos dos alunos. Contudo, as pesquisas relacionando IoT e educação são incipientes, não conferindo uma visão clara sobre como concretizar este potencial. Este artigo apresenta uma revisão sistemática da literatura para melhor evidenciar as possibilidades de conexão entre IoT e Educação, recolhendo produções e analisando o conteúdo das mesmas e agrupando-as por ano e país de publicação, contexto e nível educacional, foco dos estudos, tecnologias adotadas, aspectos metodológicos, aspectos teóricos e desenvolvimento de competências proporcionado por tais tecnologias. Constatou-se que a maioria das pesquisas foca em aspectos técnicos e utilitários da IoT, mas existem propostas com referencial teórico-metodológico de caráter pedagógico. Os resultados apontam para a necessidade de aprofundamento da interligação entre Internet das Coisas e Educação, indicam possibilidades de pesquisas futuras e a quais áreas esse aprofundamento deve atender, possibilitando a utilização desse potencial tecnológico para a promoção de novas abordagens na área de ensino e aprendizagem.
Rancang Bangun Aplikasi Kasir Pencetak Struk Belanja Kode Quick Response berbasis Website dan Android
Authors: Wardana Andre Ryadi , Hidayat Hidayat 
Published: November 2020

Paper ini memaparkan tentang rancang bangun sebuah aplikasi kasir pencetak struk belanja sebagai bukti transaksi dalam bentuk kode Quick Response (QR) guna mengatasi penggunaan kertas struk yang panjang apabila jumlah produk pada transaksi penjualan sangat banyak. Struk belanja dalam bentuk kode QR akan meringkas ukuran struk belanja berapapun jumlah produk dalam transaksi. Tahapan perancangan aplikasi menggunakan metode waterfall yang diawali dengan menganalisa kebutuhan sistem yang akan dibangun, melakukan perancangan, mengimplementasikan rancangan, dan tahapan pengujian. Aplikasi yang dibangun menggunakan platform website untuk memasukkan data pengguna, data produk dan mengolah data transaksi serta menghasilkan kode QR untuk sebuah transaksi. MySQL digunakan database untuk menyimpan data tersebut. Kode QR yang dihasilkan oleh aplikasi akan dicetak melalui printer termal. Selanjutnya, platform android digunakan untuk aplikasi yang dapat memindai kode QR struk belanja dan menampilkan rincian transaksi produk yang dimuat dalam kode QR tersebut. Hasil pengujian memperlihatkan bahwa aplikasi yang dibangun dapat berfungsi dengan baik. Aplikasi pada website dapat mengolah data dan menghasilkan kode QR transaksi. Kemudian, aplikasi Scanner QR code yang terpasang pada telepon seluler dapat memindai kode QR dan menampilkan rincian transaksi produk melalui layar telepon seluler. Kata kunci: struk belanja, kode Quick Response, scanner QR code, website, android
Sistem Rekomendasi Film Berbasis Website Dengan Metode Prototype Menggunakan Algoritma K-Nearest Neighbors (KNN)
Authors: Fitrianti Alifia Rahma , Rohmani Asih , Widjanarto Widjanarto 
Published: November 2020

Industry perfilman dunia merupakan salah satu industry yang tidak terpengaruh dengan maraknya hiburan digital seperti munculnya media social, program televisi yang beragam dan game. Industry film yang terus melakukan produksi ini semakin menambah informasi film yang melimpah di internet. Kondisi ini justru membuat para penikmat film menjadi kebingungan ketika harus memilih film kesukaannya. Sistem rekomendasi menyediakan informasi berdasarkan interaksi pengguna dan item yang telah terekam sebelumnya. Pendekatan content-based filtering merupakan salah satu pendekatan pada sistem rekomendasi yang mampu memberikan rekomendasi item berdasarkan fitur atau atribut yang memiliki kemiripan dengan item yang telah berinteraksi dengan pengguna. Penelitian ini akan membahas pembangunan sistem rekomendasi dengan metode prototype menggunakan algoritma K-Nearest Neighbors (KNN) serta pendekatan content-based filtering. K-Nearest Neighbors digunakan untuk mengklasifikasikan objek baru berdasarkan atribut dan sampel dari data training. Metode pembangunan sistem prototype memiliki beberapa tahapan yaitu communication, quick plan and modelling design, construction of prototype, serta deployment delivery and feedback. Berdasarkan hasil pengujian, sistem yang dibangun dapat memberikan rekomendasi film dan hasil pengujian akurasi yang didapatkan dengan nilai k sebesar 5 (lima) adalah sebesar 67%.Kata kunci: Sistem Rekomendasi, Content-Based Filtering, K-Nearest Neighbors, Movie
Different Pathophysiology and Outcomes of Heart Failure With Preserved Ejection Fraction Stratified by K-Means Clustering
Authors: Harada Daisuke , Asanoi Hidetsugu , Noto Takahisa , Takagawa Junya 
Published: November 2020

Background: Stratified medicine may enable the development of effective treatments for particular groups of patients with heart failure with preserved ejection fraction (HFpEF); however, the heterogeneity of this syndrome makes it difficult to group patients together by common disease features. The aim of the present study was to find new subgroups of HFpEF using machine learning.Methods: K-means clustering was used to stratify patients with HFpEF. We retrospectively enrolled 350 outpatients with HFpEF. Their clinical characteristics, blood sample test results and hemodynamic parameters assessed by echocardiography, electrocardiography and jugular venous pulse, and clinical outcomes were applied to k-means clustering. The optimal k was detected using Hartigan's rule.Results: HFpEF was stratified into four groups. The characteristic feature in group 1 was left ventricular relaxation abnormality. Compared with group 1, patients in groups 2, 3, and 4 had a high mean mitral E/e′ ratio. The estimated glomerular filtration rate was lower in group 2 than in group 3 (median 51 ml/min/1.73 m2 vs. 63 ml/min/1.73 m2p &lt; 0.05). The prevalence of less-distensible right ventricle and atrial fibrillation was higher, and the deceleration time of mitral inflow was shorter in group 3 than in group 2 (93 vs. 22% p &lt; 0.05, 95 vs. 1% p &lt; 0.05, and median 167 vs. 223 ms p &lt; 0.05, respectively). Group 4 was characterized by older age (median 85 years) and had a high systolic pulmonary arterial pressure (median 37 mmHg), less-distensible right ventricle (89%) and renal dysfunction (median 54 ml/min/1.73 m2). Compared with group 1, group 4 exhibited the highest risk of the cardiac events (hazard ratio [HR]: 19; 95% confidence interval [CI] 8.9–41); group 2 and 3 demonstrated similar rates of cardiac events (group 2 HR: 5.1; 95% CI 2.2–12; group 3 HR: 3.7; 95%CI, 1.3–10). The event-free rates were the lowest in group 4 (p for trend &lt; 0.001).Conclusions: K-means clustering divided HFpEF into 4 groups. Older patients with HFpEF may suffer from complication of RV afterload mismatch and renal dysfunction. Our study may be useful for stratified medicine for HFpEF.
Hypertension in Acromegaly in Relationship to Biochemical Control and Mortality: Global ACROSTUDY Outcomes
Authors: Vila Greisa , Luger Anton , van der Lely Aart Jan , Neggers Sebastian J. C. M. M. , Webb Susan M. , Biller Beverly M. K. , Valluri Srinivas , Hey-Hadavi Judith 
Published: November 2020

ContextHypertension is a major cardiovascular risk factor related to increased mortality in acromegaly. Surgical cure of acromegaly is associated with improvement in blood pressure levels, however little is known about the effect of pegvisomant (PEGV) treatment in patients with hypertension. This analysis evaluates outcomes in patients with hypertension and acromegaly included in ACROSTUDY.MethodsACROSTUDY is a global non-interventional surveillance study of long-term treatment with PEGV, monitoring its safety and efficacy. The cohort was retrospectively divided in two subgroups: patients with and without hypertension. Stepwise logistic regression and Kaplan-Meyer analyses were performed for testing predictors of mortality.ResultsThe total cohort included 2,090 patients with acromegaly treated with PEGV who were followed for a median of 6.8 years (range up to 12.1 years). In ACROSTUDY there were 1,344 patients with hypertension (52.3% males). This subgroup was older, had a higher BMI, and higher prevalence of diabetes, hyperlipidemia, and cardiovascular disease (CVD) when compared to patients without hypertension. During ACROSTUDY, 68 deaths were reported in the hypertension cohort, vs 10 in the cohort without hypertension. Both CVD (p&lt;0.0001) and anterior pituitary deficiencies (p=0.0105) at study entry independently predicted mortality in patients with acromegaly and hypertension; Kaplan-Meier analysis confirmed that CVD significantly impairs survival.ConclusionsHypertension is common in patients with acromegaly and significantly increases mortality, especially when there is concomitant CVD. These data suggest that treatment goals should extend beyond IGF-I normalization, and include optimisation of substitution of pituitary deficiencies and scrutinous screening and treatment of CVD.
Prognostic Impact of Hyponatremia and Hypernatremia in COVID-19 Pneumonia. A HOPE-COVID-19 (Health Outcome Predictive Evaluation for COVID-19) Registry Analysis
Authors: Ruiz-Sánchez Jorge Gabriel , Núñez-Gil Ivan J. , Cuesta Martin , Rubio Miguel A. , Maroun-Eid Charbel , Arroyo-Espliguero Ramón , Romero Rodolfo , Becerra-Muñoz Victor Manuel , Uribarri Aitor , Feltes Gisela , Trabattoni Daniela , Molina María , García Aguado Marcos , Pepe Martino , Cerrato Enrico , Alfonso Emilio , Castro Mejía Alex Fernando , Roubin Sergio Raposeiras , Buzón Luis , Bondia Elvira , Marin Francisco , López Pais Javier , Abumayyaleh Mohammad , D’Ascenzo Fabrizio , Rondano Elisa , Huang Jia , Fernandez-Perez Cristina , Macaya Carlos , de Miguel Novoa Paz , Calle-Pascual Alfonso L. , Estrada Perez Vicente , Runkle Isabelle ,   HOPE COVID-19 investigators
Published: November 2020

Dysnatremia is associated with increased mortality in patients with community-acquired pneumonia. SARS-COV2 (Severe-acute-respiratory syndrome caused by Coronavirus-type 2) pneumonia can be fatal. The aim of this study was to ascertain whether admittance dysnatremia is associated with mortality, sepsis, or intensive therapy (IT) in patients hospitalized with SARS-COV2 pneumonia. This is a retrospective study of the HOPE-COVID-19 registry, with data collected from January 1th through April 31th, 2020. We selected all hospitalized adult patients with RT-PCR-confirmed SARS-COV2 pneumonia and a registered admission serum sodium level (SNa). Patients were classified as hyponatremic (SNa &lt;135 mmol/L), eunatremic (SNa 135–145 mmol/L), or hypernatremic (SNa &gt;145 mmol/L). Multivariable analyses were performed to elucidate independent relationships of admission hyponatremia and hypernatremia, with mortality, sepsis, or IT during hospitalization. Four thousand six hundred sixty-four patients were analyzed, median age 66 (52–77), 58% males. Death occurred in 988 (21.2%) patients, sepsis was diagnosed in 551 (12%) and IT in 838 (18.4%). Hyponatremia was present in 957/4,664 (20.5%) patients, and hypernatremia in 174/4,664 (3.7%). Both hyponatremia and hypernatremia were associated with mortality and sepsis. Only hyponatremia was associated with IT. In conclusion, hyponatremia and hypernatremia at admission are factors independently associated with mortality and sepsis in patients hospitalized with SARS-COV2 pneumonia.Clinical Trial Registrationhttps://clinicaltrials.gov/ct2/show/NCT04334291, NCT04334291.
A Potential Role for Endogenous Glucagon in Preventing Post-Bariatric Hypoglycemia
Authors: Lobato Carolina B. , Pereira Sofia S. , Guimarães Marta , Hartmann Bolette , Wewer Albrechtsen Nicolai J. , Hilsted Linda , Holst Jens J. , Nora Mário , Monteiro Mariana P. 
Published: November 2020

Obesity and obesity-related diseases are major public health concerns that have been exponentially growing in the last decades. Bariatric surgery is an effective long-term treatment to achieve weight loss and obesity comorbidity remission. Post-bariatric hypoglycemia (PBH) is a late complication of bariatric surgery most commonly reported after Roux-en-Y gastric bypass (RYGB). PBH is the end result of postprandial hyperinsulinemia but additional endocrine mechanisms involved are still under debate. Our aim was to characterize entero-pancreatic hormone dynamics associated with postprandial hypoglycemia after RYGB. Individuals previously submitted to RYGB (N=23) in a single tertiary hospital presenting PBH symptoms (Sym, n=14) and asymptomatic weight-matched controls (Asy, n=9) were enrolled. Participants underwent a mixed-meal tolerance test (MMTT) to assess glucose, total amino acids (total AA), insulin, C-peptide, glucagon, glucose-dependent insulinotropic polypeptide (GIP), glucagon-like peptide-1 (GLP-1), and neurotensin (NT). We found that hypoglycemia during the MMTT was equally frequent in Sym and Asy groups (p=1.000). Re-grouped according to glucose nadir during the MMTT (Hypo n=11 vs NoHypo n=12; nadir &lt;3.05 mmol/l vs ≥3.05 mmol/l), subjects presented no differences in anthropometric (BMI: p=0.527) or metabolic features (HbA1c: p=0.358), yet distinct meal-elicited hormone dynamics were identified. Postprandial glucose excursion and peak glucose levels were similar (p&gt;0.05), despite distinct late glycemic outcomes (t=60 min and t=90 min: p&lt;0.01), with overall greater glycemic variability in Hypo group (minimum-to-maximum glucose ratio: p&lt;0.001). Hypo group meal-triggered hormone profile was characterized by lower early glucagon (t=15 min: p&lt;0.01) and higher insulin (t=30 min: p&lt;0.05, t=45 min: p&lt;0.001), C-peptide (t=30 min: p&lt;0.01, t=45 min: p&lt;0.001, t=60 min: p&lt;0.05), and GLP-1 (t=45 min: p&lt;0.05) levels. Hyperinsulinemia was an independent risk factor for hypoglycemia (p&lt;0.05). After adjusting for hyperinsulinemia, early glucagon correlated with glycemic nadir (p&lt;0.01), and prevented postprandial hypoglycemia (p&lt;0.05). A higher insulin to glucagon balance in Hypo was observed (p&lt;0.05). No differences were observed in total AA, GIP or NT excursions (p&gt;0.05). In sum, after RYGB, postprandial hyperinsulinemia is key in triggering PBH, but a parallel and earlier rise in endogenous glucagon might sustain the inter-individual variability in glycemic outcome beyond the effect of hyperinsulinism, advocating a potential pivotal role for glucagon in preventing hyperinsulinemic hypoglycemia.
IGLR-2, a Leucine-Rich Repeat Domain Containing Protein, Is Required for the Host Defense in Caenorhabditis elegans
Authors: Kuo Cheng-Ju , Hsu Ya-Chu , Wang Sin-Tian , Liou Bang-Yu , Lim Serene Boon-Yuean , Chen Yi-Wei , Chen Chang-Shi 
Published: November 2020

EnterohemorrhagicEscherichia coli(EHEC), a human pathogen, also infectsCaenorhabditis elegans. We demonstrated previously thatC. elegansactivates the p38 MAPK innate immune pathway to defend against EHEC infection. However, whether aC. eleganspattern recognition receptor (PRR) exists to regulate the immune pathway remains unknown. PRRs identified in other metazoans contain several conserved domains, including the leucine-rich repeat (LRR). By screening a focused RNAi library, we identified the IGLR-2, a transmembrane protein containing the LRR domain, as a potential immune regulator inC. elegans. Our data showed thatiglr-2regulates the host susceptibility to EHEC infection. Moreover,iglr-2is required for pathogen avoidance to EHEC. Theiglr-2overexpressed strain, which was more resistant to EHEC originally, showed hypersusceptibility to EHEC upon knockdown of the p38 MAPK pathway. Together, our data suggested thatiglr-2plays an important role inC. elegansto defend EHEC by regulating pathogen-avoidance behavior and the p38 MAPK pathway.
Identification of Mutated Peptides in Bladder Cancer From Exomic Sequencing Data Reveals Negative Correlation Between Mutation-Specific Immunoreactivity and Inflammation
Authors: Wang Chen , Ding Yu , Liu Yuanyong , Zhang Qingchen , Xu Shiqiang , Xia Liliang , Duan Huangqi , Wang Shujun , Ji Ping , Huang Weiren , Zhao Guoping , Cao Zhiwei , Shen Haibo , Wang Ying 
Published: November 2020

Introduction and ObjectiveNeoantigen-based immunotherapy is one of the breakthroughs in cancer immunotherapy. Benefit from the Cancer Genome Atlas database, we intended to identify mutant peptides with neoantigen property in bladder cancer (BC). Correlations between the immunoreactivity of candidate neoantigens and clinical manifestations were further analyzed.MethodsHLA-A*02:01 restricted mutant (MT) and wildtype (WT) peptides were predicted by using whole exome sequencing data of 412 BC patients in the TCGA database. Binding affinity to HLA-A2 molecules was determined by using T2 cell-based binding assay. The immunoreactivity to WT and MT peptides in HLA-A2+ BC patients was determined by using an ELISPOT assay upon in vitro stimulation with MT and WT peptides individually. Clinical relevance to peptide-specific immunoreactivity was analyzed by Pearson correlation analysis. The disease free survival (DFS) curves were plotted using the Kaplan–Meier method in BC patients with or without mutations and compared using the log-rank test online.ResultsFifty-seven HLA-A*02:01 restricted WT and MT peptides were selected based on predicted high affinity and expression frequency, among which 12 MT peptides from 12 individual genes exhibited strong affinity to HLA-A2 molecules when compared to WT counterparts. MT peptides induced more peptide-specific IFNγ spot forming units (SFUs) than WT counterparts in HLA-A2+ BC patients upon in vitro stimulation. They were negatively correlated to the counts of peripheral leukocytes and platelets. Patients with higher C-reactive protein level exhibited lower immunoreactivity to MT peptides. Combination of MT peptides from 6 genes, including CDKN1AG61V, RHOBP75L, DDB1S25L, AHNAKD4855Y, ANP32AS56L and MKI67H84L covered 47.5% of the patients under investigation. Patients harboring combinational mutations in these genes were associated with a longer DFS according to the cBioportal online analysis.ConclusionTwelve HLA-A*02:01 restricted MT peptides have been identified exhibiting higher binding affinity to HLA-A2 molecules and stronger immunoreactivity than WT counterparts in BC patients. Combination of MT peptides from six genes might be potential as neoantigen candidates in cancer immunotherapy against BC in the future. Inflammatory modulation is inclined to be a strategy to enhance the efficacy of neoantigen-based immunotherapy.
Innate Immune Responses to Chimpanzee Adenovirus Vector 155 Vaccination in Mice and Monkeys
Authors: Collignon Catherine , Bol Vanesa , Chalon Aurélie , Surendran Naveen , Morel Sandra , van den Berg Robert A. , Capone Stefania , Bechtold Viviane , Temmerman Stéphane T. 
Published: November 2020

Replication-deficient chimpanzee adenovirus (ChAd) vectors represent an attractive vaccine platform and are thus employed as vaccine candidates against several infectious diseases. Since inducing effective immunity depends on the interplay between innate and adaptive immunity, a deeper understanding of innate immune responses elicited by intramuscularly injected ChAd vectors in tissues can advance the platform’s development. Using different candidate vaccines based on the Group C ChAd type 155 (ChAd155) vector, we characterized early immune responses in injected muscles and draining lymph nodes (dLNs) from mice, and complemented these analyses by evaluating cytokine responses and gene expression patterns in peripheral blood from ChAd155-injected macaques. In mice, vector DNA levels gradually decreased post-immunization, but local transgene mRNA expression exhibited two transient peaks [at 6 h and Day (D)5], which were most obvious in dLNs. This dynamic pattern was mirrored by the innate responses in tissues, which developed as early as 1–3 h (cytokines/chemokines) or D1 (immune cells) post-vaccination. They were characterized by a CCL2- and CXCL9/10-dominated chemokine profile, peaking at 6 h (with CXCL10/CCL2 signals also detectable in serum) and D7, and clear immune-cell infiltration peaks at D1/D2 and D6/D7. Experiments with a green fluorescent protein-expressing ChAd155 vector revealed infiltrating hematopoietic cell subsets at the injection site. Cell infiltrates comprised mostly monocytes in muscles, and NK cells, T cells, dendritic cells, monocytes, and B cells in dLNs. Similar bimodal dynamics were observed in whole-blood gene signatures in macaques: most of the 17 enriched immune/innate signaling pathways were significantly upregulated at D1 and D7 and downregulated at D3, and clustering analysis revealed stronger similarities between D1 and D7 signatures versus the D3 signature. Serum cytokine responses (CXCL10, IL1Ra, and low-level IFN-α) in macaques were predominantly observed at D1. Altogether, the early immune responses exhibited bimodal kinetics with transient peaks at D1/D2 and D6/D7, mostly with an IFN-associated signature, and these features were remarkably consistent across most analyzed parameters in murine tissues and macaque blood. These compelling observations reveal a novel aspect of the dynamics of innate immunity induced by ChAd155-vectored vaccines, and contribute to ongoing research to better understand how adenovectors can promote vaccine-induced immunity.