bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Use of Innominate Artery Cannulation for Antegrade Cerebral Perfusion in Aortic Dissection
Authors: Payabyab Eden C , Hemli Jonathan M. , Mattia Allan , Kremers Alex , Vatsia Sohrab K. , Scheinerman S. Jacob , Mihelis Efstathia A , Hartman Alan R. , Brinster Derek R. 
Published: March 2020

Abstract
Background Direct cannulation of the innominate artery for selective antegrade cerebral perfusion (SACP) has been shown to be safe in elective proximal aortic reconstructions. We sought to evaluate the safety of this technique in acute aortic dissection. Methods A multi-institutional retrospective review was undertaken of patients who underwent proximal aortic reconstruction for Stanford type A dissection between 2006 and 2016. Those patients who had direct innominate artery cannulation for SACP were selected for analysis. Results Seventy-five patients underwent innominate artery cannulation for ACP for Stanford Type A Dissections. Isolated replacement of the ascending aorta was performed in 36 patients (48.0%), concomitant aortic root replacement was required in 35 patients (46.7%), of whom 7 had a valve-sparing aortic root replacement. Other procedures included frozen elephant trunk (n = 11 (14.7%)), coronary artery bypass grafting (n = 20 (26.7%)), and peripheral arterial bypass (n = 4 (5.3%)). Mean hypothermic circulatory arrest time was 19 ± minutes. Thirty-day mortality was 14.7% (n = 11). Perioperative stroke occurred in 7 patients (9.3%). Conclusions This study is the first comprehensive review of direct innominate artery cannulation through median sternotomy for SACP in aortic dissection. Our experience suggests that this strategy is a safe and effective technique compared to other reported methods of cannulation and ACP for delivering selective ACP in these cases.
Recent epidemiology of patients with Gastro-Entero-Pancreatic Neuroendocrine Neoplasms (GEP-NEN) in Japan: a population-based study
Authors: Masui Toshihiko , Ito Tetsuhide , Komoto Izumi , Uemoto Shinji 
Published: March 2020

Abstract
Background The worldwide prevalence and incidence of neuroendocrine neoplasms (NEN) have been increasing recently, although few studies have analyzed data on the current situation of NENs in Japan. Here, the Japan Neuroendocrine Tumor Society (JNETS) planned to investigate the recent incidence and distribution of these tumors using data from the national cancer registry started in 2016. This study examined the incidence and distribution of primary sites as well as disease progression from this population-based registry.Methods A retrospective, population-based study using data from the national cancer registry in Japan (NCR) was conducted to evaluate patients with gastro-entero-pancreatic NEN (GEP-NEN) in 2016. Associated population data were used to determine annual age-adjusted incidences.Results A total of 6735 individuals were diagnosed with GEP-NEN in Japan in 2016. Annual onset incidence was 0.70/100,000 for pancreatic NEN and 2.84/100,000 for gastrointestinal NEN. NEN in the ileum accounted for only 1% of total GEP-NENs in Japan. Most NENs in the esophagus or lungs were neuroendocrine carcinomas (NECs), while the majority of those in the duodenum, ileum, appendix and rectum were grade 1 neuroendocrine tumors (NETs). Median age at initial diagnosis was in between 60 to 65. Initial progression of the tumor was mostly limited to local for tumors in the duodenum, appendix and rectum, while those in the esophagus, stomach and colon tended to show distant metastasis. In Japan, initial treatment for GEP-NENs was resection even if the tumor was NEC.Conclusions This is the first report of a national registry-based incidence and distribution of GEP-NEN in Japan. These data will serve as an important first step to determining the exact etiology and trends for this pathology in Japan.
Enhanced brain-derived neurotrophic factor distribution in differentiated human neuroblastoma SH-SY5Y cells by PEG-ylated protein nanoparticles: model of application in neurodegenerative diseases
Authors: Dąbkowska Maria , Łuczkowska Karolina , Rogińska Dorota , Sobuś Anna , Wasilewska Monika , Ulańczyk Zofia , Machaliński Bogusław 
Published: March 2020

Abstract
Brain-derived neurotrophic factor (BDNF) is essential for the development and function of human neurons, therefore it is a promising target for neurodegenerative disorders treatment. Here, we studied BDNF-based electrostatic complex with dendrimer nanoparticles encapsulated in polyethylene glycol (PEG) in injured, differentiated neuroblastoma SH-SY5Y cells, a model of neurodegenerative mechanisms. PEG layer was adsorbed at dendrimer-protein core nanoparticles to decrease their cellular uptake and to reduce BDNF-serum proteins interaction for prolonged time. Cytotoxicity and confocal microscopy analysis revealed PEG-ylated BDNF-dendrimer nanoparticles can be used for continuous neurotrophic factor delivery enhancing its distribution into cells over 24 hours without toxic effect. We offer reliable electrostatic route for efficient encapsulation and controlled transport of fragile therapeutic proteins without any covalent cross-linker; this could be considered as safe drug delivery system. Understanding of polyvalent BDNF interactions with dendrimer core nanoparticles offers new possibilities for design of well-ordered protein drug delivery systems.
Smart@Home – Supporting safety and mobility of elderly and care dependent people in their own homes through the use of technical assistance systems and conventional mobility supporting tools: A cross-sectional survey
Authors: Jachan Deborah Elisabeth , Strube-Lahmann Sandra , Müller-Werdan Ursula , Lahmann Nils Axel 
Published: March 2020

Abstract
BACKGROUND: The use of technical solutions and conventional mobility aids can support the independence of people into old age in their own homes. However, we found relatively few empirical investigations on the effects and costs of these systems. METHODS: The aim of the study was to investigate usability, user satisfaction and the correlation between costs and benefits of different built-in smart home solutions and conventional mobility supporting tools in the home of elderly, partially care-dependent tenants (>65 years). A cross-sectional survey was conducted from February to March 2018 with tenants of a housing association in apartments equipped with smart home technology and conventional mobility supporting tools. The response rate in the intervention group was n=37 persons (out of 46 tenants with installed smart home and conventional solutions) and in the control group n=64 persons (out of 100 tenants without built-in smart home and conventional solutions). Data were collected by a written questionnaire regarding usability and satisfaction of the tenants with the built-in smart home solutions and conventional mobility supporting tools. In addition, both the intervention and the control group were asked general questions about communication, safety and how to deal with the need for long-term care in their own living environment. RESULTS: Results showed that with regard to usability, satisfaction and price performance ratio of the installed smart home solutions, the installation of the corresponding solutions with an overall score of 1.41 was mostly positively evaluated by the tenants. Overall, users rated the installed smart home solutions better than the conventional mobility supporting tools (mobility aids, such as handholds and increased balcony floor level). CONCLUSIONS: Analysis of the price performance ratio showed that smart home solutions are generally more expensive than conventional tools, but also contribute significantly to an increased security of the tenants, and thus may enable longer living in a familiar environment. We recommend modularized offers consisting of various components of smart home solutions, since this significantly reduces installation costs and allows for an individual composition according to requirements. Moreover, smart home solutions should be considered to be listed as medical aids.
Association of tau accumulation and atrophy in mild cognitive impairment: a longitudinal study
Authors: Xu Gang , Zheng Shuzhan , Zhu Zhilong , Yu Xiaofeng , Jiang Jian , Jiang Juanjuan , Chu Zhaohu 
Published: March 2020

Abstract
Background To examine the patterns of longitudinal tau accumulation and cortical atrophy and their association in patients with mild cognitive impairment (MCI). Methods We collected 23 participants (60-89 years old, 11 male/12 female) with MCI from the Alzheimer’s Disease Neuroimaging Initiative database. All participants underwent 18 F flortauirpir (FTP) positron emission tomography (PET) and structural magnetic resonance imaging (MRI) scans at the baseline and follow-up visits (12-36 months). General linear models with covariates (baseline age, sex) were used to detect brain areas of significant tau accumulation and atrophy over time. The mediation analysis was employed to explore the potential reason for sequential biomarker changes in MCI progression, adjusting for baseline age, sex, education.Results Voxelwise tau accumulation in MCI patients was predominantly located in inferior temporal, middle temporal, parietal cortex, posterior cingulate, precuneus as well as temporo-parietal regions ( P < 0.001), and MRI atrophy included inferior-middle temporal, parietal lobe, cerebellum and precuneus ( P < 0.001). Longitudinal FTP accumulation was moderately associated with MRI cortical atrophy ( r = 0.409, 95% CI: 0.405-0.414, P < 0.01). Regional analyses indicated significant bivariate associations between MRI cortical atrophy and FTP accumulation (baseline FTP cortical uptake and longitudinal FTP change). The result of the mediation analysis showed the relationship between baseline FTP uptake and longitudinal cortical atrophy was partly mediated by the longitudinal FTP cortical change (indirect effect: 0.0107, P = 0.04).Conclusions Our finding provides a preliminary description of the patterns of longitudinal FTP accumulation and cortical atrophy in MCI progression, and MCI patients with high tau binding level show increase risk of longitudinal tau accumulation, atrophy and cognitive decline.
Effects of different autophagy inhibitors on treatment with ATO/ATRA in KG-1 and HL-60 leukemia cells
Authors: Haghi Atousa , Salemi Mahdieh , Fakhimahmadi Aila , Mohammadikian Mahnaz , Yousefi Hassan , Rahmati Marveh , Mohammadi Saeed , Ghavamzadeh Ardeshir , Moosavi Mohammad Amin , Nikbakht Mohsen 
Published: March 2020

Abstract
Background : Few current autophagy inhibitors may have beneficial effects for acute myeloid leukemia (AML) patients in clinical phases. However, there is a strong need to figure out which settings should be activated or inhibited in autophagy pathway to prevail drug resistance and improve current treatment options in leukemia. This study aimed to compare the effects of 3 well-known inhibitors of autophagy (3-MA, BafA1 and HCQ) in human leukemia KG1 and HL-60 cells exposed to ATO and/or ATRA. Methods : Cell proliferation and cytotoxicity of KG-1 and HL-60 cells were examined by MTT assay. Autophagy was studied by evaluating the development of acidic vesicular organelles, and the autophagosomes formation was investigated by acridine orange staining and transmission electron microscopy. Gene and protein expression levels of autophagy markers ( ATGs , p62/SQSTM1 and LC-3B) were also performed by qPCR and western blotting, respectively. The rate of apoptosis (annexin-V/PI staining) and cell cycle were evaluated using flow cytometry. Result: We compared the cytotoxic and apoptotic effects of arsenic trioxide (ATO) and/or all-trans retinoic acid (ATRA) in KG1 and HL-60 cells, and demonstrated that some autophagy markers can be upregulated in this context. Also, autophagy blockers HCQ and/or BafA1 could potentiate the cytotoxic effects of ATO/ATRA, which were more pronounced in KG-1 cells compared with HL-60 cell line. Conclusion: This study showed the involvement of autophagy during treatment of KG1 and HL-60 cells with ATO/ATRA.also, this study propose was to propose that combination therapy of ATO/ATRA with the autophagy inhibitor HCQ can be considered as a more effective strategy for targeting leukemic KG-1 cells. Key words: Autophagy, AML, ATO, ATRA
Changes in coagulation status as measured by thromboelastography in patients undergoing revision total hip arthroplasty
Authors: Yang Jingye , Zhu Sizheng , Qin Leilei , Wang Jiawei , Huang Jiaxing , Sandiford Steve , Li Feilong , Gong Xuan , Liang Xi , Huang Wei , Hu Ning 
Published: March 2020

Abstract
Background: Venous thromboembolism events (VTEs) continue to be of the most widespread severe complication following total hip arthroplasty (THA) and total knee arthroplasty (TKA). However, there are no optimal accurate monitoring methods to assess the changes in coagulability after anticoagulation and anti-fibrinolysis during the perioperative period. Therefore, the objective of this study is to determine changes in coagulability as measured by thromboelastography (TEG) following revision total hip arthroplasty when the patients received rivaroxaban and tranexamic acid in perioperative period during enhanced recovery after surgery (ERAS). Methods: We retrospectively reviewed 70 revision TKA patients (mean age 63.69±10.17 years). Perioperative management of each patient was conducted in accordance with ERAS. The patients received tranexamic (TXA) to control perioperative bleeding. TEG was performed pre-operatively and on post-operative days (POD) 1, 3, 5 and 7. TEG-hypercoagulability was classified into three types: enzymatic hypercoagulability, platelet hypercoagulability and mixed hypercoagulability. Screening for coagulation-related complications at three months of follow-up. Results: The mean duration of surgery was 2.91±0.99 h. the mean intraoperative blood loss of patients was 486.43±346.92 ml. And 55.71% (39) patients received transfusion, the mean blood transfusion volume was 482.86 ± 458.79 ml. There only were 4 (5.71%) patients who suffered postoperative coagulation-related complications. 1 patient with hypercoagulable on preoperative developed intramuscular venous thrombosis at 1 month postoperatively. 1 patient with hypercoagulability at POD5 and POD7 suffered melena at POD5. 2 patients with hypocoagulability developed ecchymosis at POD3. The proportion of postoperative hypercoagulable state is gradually increasing. The distribution of different hypercoagulable states on the postoperative day (POD) 5 and 7 were significantly different from that pre-operation (Pre) and POD1 (POD5 vs Pre: p=0.011; POD5 vs POD1: p=0.001; POD7 vs Pre: p=0.001; POD7 vs POD5: p<0.001). We found 32.86%(23) revision THA with hypercoagulable state on POD7.For 78.26%(18) of these patients there was mixed hypercoagulability. Conclusions: In ERAS, thromboelastography was an effective way to identify hypercoagulable state in patients undergoing revision hip arthroplasty, and mixed hypercoagulability is the predominant hypercoagulable state following revision hip arthroplasty. In addition, it is very important to develop an individualized coagulation management program.
Importance of lipid ratios for predicting intracranial atherosclerotic stenosis
Authors: Yang Wen-Song , Li Rui , Shen Yi-Qing , Wang Xing-Chen , Liu Qing-Jun , Wang Hai-Yang , Li Qi , Yao Guo-En , Xie Peng 
Published: March 2020

Abstract
Background: We wished to investigate the association of lipid ratios with intracranial atherosclerotic stenosis (ICAS) in a Chinese population. Methods: We included 658 consecutive patients with ischemic stroke in our cross-sectional study. Intracranial and extracranial arteries were evaluated for atherosclerotic stenosis using digital subtraction angiography or computed tomography angiography. Lipid ratios [total cholesterol (TC)/high-density lipoprotein-cholesterol (HDL-C), triglycerides (TG)/HDL-C, low-density lipoprotein-cholesterol (LDL-C)/HDL-C, non-high-density lipoprotein-cholesterol (non-HDL-C)/HDL-C, remnant cholesterol (RC)/HDL-C, apolipoprotein B (apo B)/apolipoprotein A-I (apo A-I), and apo B/HDL-C] were calculated. Results : Ratios of TC/HDL-C, LDL-C/HDL-C, RC/HDL-C, non-HDL-C/HDL-C, apo B/HDL-C and apo B/apo A-I were associated significantly with ICAS but not with extracranial atherosclerotic stenosis after adjustment for confounding factors. Analyses of receiver operating characteristic (ROC) curves revealed the apo B/apo A-I ratio have the highest area under the ROC curve (AUC) value for a lipid level alone and for lipid ratios (AUC = 0.588). Lipid ratios had higher AUC values than those for a lipid level alone for identifying ICAS. Conclusion : The ratios of TC/HDL-C, LDL-C/HDL-C, RC/HDL-C, non-HDL-C/HDL-C apo B/HDL-C and apo B/apo A-I were related significantly to ICAS risk. Compared with the other variables tested, the apo B/apo A-I ratio appeared to be better discriminator for identifying ICAS risk.
Variation in thyroid volumes due to differences in the measured length or area of the cross-sectional plane: A validation study of the ellipsoid approximation method using CT images.
Authors: Fujita Naotoshi , Kato Katsuhiko , Abe Shinji , Naganawa Shinji 
Published: March 2020

Abstract
Background This study examined the variation in the thyroid volume determined by the ellipsoid approximation method due to differences in the measured length or area of the cross-sectional plane of CT images. Methods Forty-five patients with Graves' diseasewere included in this retrospective study. We designated the three-dimensional thyroid volumes extracted manually (VCT) as the reference data and calculated five approximate volumes for comparison: (1) the mean volume of 8100 different thyroid volumes depending on the diameter of the cross-sectional plane at the midpoint of the major axis, (Vellipsoid,mean); (2) the volume using the maximum diameter and its orthogonal diameter, (Vellipsoid,maxlength); (3) the maximum (Vellipsoid,maxvolume) and (4) minimum (Vellipsoid,minvolume) of the 8100 thyroid volumes; and (5) the volume determined with an equivalent circle diameter, (Vellipsoid,Heywood). Results Thyroid volumes obtained via the ellipsoid approximation method varied depending on the diameter of the cross-sectional plane and included a mean error of approximately 20%, while the concordance correlation coefficient (CCC) differed for each approximate volume. Among these volumes, Vellipsoid,meanand Vellipsoid,Heywoodwere in good agreement with VCT, according to single regression analyses and the resultant CCC values, with mean errors of 7.0% and 2.5%, respectively. Conclusion WhileVellipsoid,Heywoodapproximated thyroid volumes with vastly reduced errors, we recommend utilizing three-dimensional thyroid volumetry if measurement accuracy is required.
Efficacy of lower dose Pirfenidone for idiopathic pulmonary fibrosis in real practice: a retrospective cohort study
Authors: Hwang Hyeontaek , Lee Jung-Kyu , Choi Sun Mi , Lee Yeon Joo , Cho Young-Jae , Yoon Ho Il , Lee Jae Ho , Lee Choon-Taek , Kim Young Whan , Park Jong Sun 
Published: March 2020

Abstract
Background Pirfenidone slows the progression of idiopathic pulmonary fibrosis. We investigated the efficacy and safety of pirfenidone by dose and disease severity in real-world patients with idiopathic pulmonary fibrosis.Methods This multi-centre retrospective cohort study investigated 338 patients treated with pirfenidone between July 2012 and March 2018. Demographics, pulmonary function, mortality, and pirfenidone-related adverse events were recorded. Efficacy was analysed according to pirfenidone dose and disease severity using linear mixed-effects models to assess the annual decline rate of forced vital capacity (FVC) and diffusing capacity (DL CO ) of the lungs for carbon monoxide.Results The mean %FVC predicted and %DL COpredicted were 72.6±13.1% and 61.4±17.9%, respectively. Pirfenidone treatment lasted 16.1±9.0 months. In the standard-dose (1800 mg/day) group, the mean %FVC predicted was -6.56% (95% CI -9.26, -3.87) per year pre-pirfenidone treatment, but -4.43% (95% CI -5.87, -3.00) per year post-treatment. In the lower-dose group, the mean %FVC predicted was -4.96% (95% CI -6.82, -3.09) per year pre-pirfenidone treatment, but -1.79% (95% CI -2.75, -0.83) per year post-treatment. The FVC decline rate was significantly reduced regardless of GAP stage. However, the DL CO decline rate was significantly reduced in the GAP stage II–III group, but not stage I group. Adverse events and mortality were similar across dose groups, but were more frequent in the GAP stage II–III group than in the stage I group.Conclusions The effect of pirfenidone on reducing disease progression persisted even with a consistent lower dose of pirfenidone.Trial registration Retrospectively registered
Prediction of transcription factor binding site across cell-types using attention-based deep neural networks
Authors: Chen Chen , Hou Jie , Shi Xiaowen , Yang Hua , Birchler James A. , Cheng Jianlin 
Published: March 2020

Abstract
Background Due to the complexity of the biological systems, the prediction of the potential DNA binding sites for transcription factors remains a difficult problem in computational biology. Genomic DNA sequences and experimental results from parallel sequencing provide available information about the affinity and accessibility of genome and are commonly used features in binding sites prediction. The attention mechanism in deep learning has shown its capability to learn long-range dependencies from sequential data, such as sentences and voices. Until now, no study has applied this approach in binding site inference from massively parallel sequencing data. The successful applications of attention mechanism in similar input contexts motivate us to build and test new methods that can accurately determine the binding sites of transcription factors. Results In this study, we propose a novel tool (named DeepGRN) for transcription factors binding site prediction based on the combination of two components: single attention module and pairwise attention module. The performance of our methods is evaluated on the ENCODE-DREAM in vivo Transcription Factor Binding Site Prediction Challenge datasets. The results show that DeepGRN achieves higher unified scores in 6 of 13 targets than any of the top four methods in the DREAM challenge. We also demonstrate that the attention weights learned by the model are correlated with potential informative inputs, such as DNase-Seq coverage and motifs, which provide possible explanations for the predictive improvements in DeepGRN. Conclusions DeepGRN can automatically and effectively predict transcription factor binding sites from DNA sequences and DNase-Seq coverage. Furthermore, the visualization techniques we developed for the attention modules help to interpret how critical patterns from different types of input features are recognized by our model.