bluuebunny/crossref_metadata_2025_split huggingface dataset:
Antibiotic-induced alterations and repopulation dynamics of yellowtail kingfish microbiota
Authors: Legrand Thibault Philippe Raymond Albert , Catalano Sarah R. , Wos-Oxley Melissa L. , Wynne James W. , Weyrich Laura S. , Oxley Andrew P.A. 
Published: June 2020

Abstract
Background The use of antibiotics in aquaculture is a common infection treatment and is increasing in some sectors and jurisdictions. While antibiotic treatment can negatively shift gut bacterial communities, recovery and examination of these communities in fish of commercial importance is not well documented. Examining the impacts of antibiotics on farmed fish microbiota is fundamental for improving our understanding and management of healthy farmed fish. This work assessed yellowtail kingfish ( Seriola lalandi ) skin and gut bacterial communities after an oral antibiotic combination therapy in poor performing fish that displayed signs of enteritis over an 18-day period. In an attempt to promote improved bacterial re-establishment after antibiotic treatment, faecal microbiota transplantation (FMT) was also administered via gavage or in the surrounding seawater, and its affect was evaluated over 15 days post-delivery. Results Antibiotic treatment greatly perturbed the global gut bacterial communities of poor-performing fish – an effect that lasted for up to 18 days post treatment. This perturbation was marked by a significant decrease in species diversity and evenness, as well as a concomitant increase in particular taxa like an uncultured Mycoplasmataceae sp., which persisted and dominated antibiotic-treated fish for the entire 18-day period. The skin-associated bacterial communities were also perturbed by the antibiotic treatment, notably within the first 3 days; however, this was unlike the gut, as skin microbiota appeared to shift towards a more ‘normal’ (though disparate) state after 5 days post antibiotic treatment. FMT was only able to modulate the impacts of antibiotics in some individuals for a short time period, as the magnitude of change varied substantially between individuals. Some fish maintained certain transplanted gut taxa (i.e. present in the FMT inoculum; namely various Aliivibrio related ASVs) at Day 2 post FMT, although these were lost by Day 8 post FMT. Conclusion As we observed notable, prolonged perturbations induced by antibiotics on the gut bacterial assemblages, further work is required to better understand the processes/dynamics of their re-establishment following antibiotic exposure. In this regard, procedures like FMT represent a novel approach for promoting improved microbial recovery, although their efficacy and the factors that support their success requires further investigation.
WITHDRAWN: Treatment for Lumbar Spinal Stenosis Secondary to Ligamentum Flavum Hypertrophy Using Percutaneous Endoscopy through Interlaminar Approach: A Retrospective Study
Authors: Liu Yi , Qi Yingjie , Diaty Diarra Mohamed , Zheng Guanglei , Shen Xiaoqiang , Lin Shangben , Chen Jiaqi , Song Yongwei , Gu Xiaomin 
Published: June 2020

Abstract

Background: This paper is to describe percutaneous endoscopy in the treatment of lumbar spinal stenosis secondary to ligamentum flavum hypertrophy targeted. To investigate the efficacy and safety of percutaneous endoscopy in the treatment of this kind of lumbar spinal stenosis in elderly patients.Method: A retrospective analysis of 40 elderly patients with lumbar spinal stenosis secondary to ligamentum flavum hypertrophy admitted between January 2016 and January 2018 was performed. According to different surgical methods, they were divided into two groups: the control group and the endoscopy group (interlaminar approach), 20 people per group. There were 9 males and 11 females in the control group; the age of patients was 65.65±4.44years, and the average disease duration was 4.55±1.85 years. Besides, There were 10 males and 10 females in the endoscopy group; the age of patients was 67.30±4.23years, and the average disease duration was 4.95±2.04 years. Collect and count surgical-related indicators, preoperative and postoperative radiologic findings, incision visual analogue scale (VAS), lumbar and leg pain VAS, lumbar Japanese Orthopaedic Association (JOA) and Oswestry disability index (ODI) scores of all patients.Result: A series of surgical indicators (including the operation time, the quantity of bleeding and postoperative hospital stay) in the endoscopy group was significantly lower than that in the control group (p < 0.05). The incision VAS score in the endoscopy group was also significantly lower than that in the control group at each time after surgery (p < 0.05). Besides, compared with the control group, in the endoscopy group, the leg pain VAS score and lumbar ODI score after surgery were significantly decreased (p < 0.05). Compared with the control group, in the endoscopy group, the lumbar JOA score was significantly higher (p < 0.05).Conclusion: Percutaneous endoscopic technique is a small trauma, quick recovery, safe and effective minimally invasive surgery for patients with lumbar spinal stenosis secondary to ligamentum flavum hypertrophy.
Berberine alters gut microbial function through modulation of bile acids
Authors: Wolf Patricia , Devendran Saravanan , Doden Heidi L. , Ly Lindsey K. , Moore Tyler , Takei Hajime , Nittono Hiroshi , Murai Tsuyoshi , Kurosawa Takao , Chlipala George E. , Green Stefan J. , Kakiyama Genta , Kashyap Purna , McCracken Vance J. , Gaskins H. Rex , Gillevet Patrick M. , Ridlon Jason M. 
Published: June 2020

Abstract
Background: Berberine (BBR) is a plant-based nutraceutical that has been used for millennia to treat diarrheal infections and in contemporary medicine to improve patient lipid profiles. Reduction in lipids, particularly cholesterol, is achieved partly through up-regulation of bile acid synthesis and excretion into the Gastrointestinal tract. The efficacy of BBR is also thought to be dependent on structural and functional alterations of the gut microbiome. However, knowledge of the effects of BBR on gut microbiome communities is currently lacking. Distinguishing indirect effects of BBR on bacteria through altered bile acid profiles is particularly important in understanding how dietary nutraceuticals alter the microbiome. Results: Germfree mice were colonized with a defined minimal gut bacterial consortium capable of functional bile acid metabolism (Bacteroides vulgatus, Bacteroides uniformis, Parabacteroides distasonis, Bilophila wadsworthia, Clostridium hylemonae, Clostridium hiranonis, Blautia producta; B4PC2). Multi-omics (bile acid metabolomics, 16S rDNA sequencing, cecal metatranscriptomics) were performed in order to provide a simple in vivo model from which to identify network-based correlations between bile acids and bacterial transcripts in the presence and absence of dietary BBR. Significant alterations in network topology and connectedness in function were observed, despite similarity in gut microbial relative abundance between control and BBR treatment. BBR increased cecal bile acid concentrations and altered the taurocholic acid:tauro-β-muricholic acid ratio in the liver. Overall, analysis of correlation networks indicates both bacterial species-specific responses to BBR, as well as functional commonalities among species, such as up-regulation of Na+/H+ antiporter, cell wall synthesis/repair, carbohydrate metabolism and amino acid metabolism. Bile acid concentrations in the gastrointestinal (GI) tract increased significantly during berberine treatment and developed extensive correlation networks with expressed genes in the B4PC2 community. Conclusions: This work has important implications for interpreting the effects of berberine on structure and function of the complex gut microbiome, which may lead to targeted pharmaceutical interventions aimed to achieve the positive physiological effects previously observed with berberine supplementation.
Treatment response lowers tumor symptom burden in recurrent and/or metastatic head and neck cancer
Authors: Hecht Markus , Hahn Dennis , Wolber Philipp , Hautmann Matthias G. , Reichert Dietmar , Weniger Steffi , Belka Claus , Bergmann Tobias , Göhler Thomas , Welslau Manfred , Große-Thie Christina , Guntinas-Lichius Orlando , Gruen Jens von der , Balermpas Panagiotis , Orlowski Katrin , Messinger Diethelm , Stenzel Karsten G. , Fietkau Rainer 
Published: June 2020

Abstract
Background
Head and neck squamous cell cancer (HNSCC) frequently causes severe symptoms that may be reduced, when the tumor is successfully treated. The SOCCER trial studied the association of treatment response with patient reported tumor symptom burden in first line treatment of recurrent and/or metastatic HNSCC.
Methods
In this prospective, multi-center, non-interventional trial patients were treated either with platinum-based chemotherapy and cetuximab or radiotherapy and cetuximab. Tumor symptom burden was assessed every four weeks with a questionnaire containing ten visual analogue scales (VAS, range 0-100), which were summarized to the overall VAS score.
Results
470 patients were registered in 97 German centers. A total of 315 patients with at least the baseline and one subsequent questionnaire were available for analysis. Changes in the VAS score were rated as absolute differences from baseline. Negative values indicate improvement of symptoms. The overall VAS score improved significantly at the first post-baseline assessment in responders (-2.13 vs. non-responders + 1.15, p = 0.048), and even more for the best post-baseline assessment (-7.82 vs. non-responders − 1.97, p = 0.0005). The VAS for pain (-16.37 vs. non-responders − 8.89, p = 0.001) and swallowing of solid food (-16.67 vs. non-responders − 5.06, p = 0.002) improved significantly more in responders (best post-baseline assessment). In the multivariable Cox regression analysis, worse overall VAS scores were associated with worse overall survival (hazard ratio for death 1.12 per 10 points increment on the overall VAS scale, 95% CI 1.05–1.20, p = 0.0009)
Conclusion
In unselected patients beyond randomized controlled trials, treatment response lowers tumor symptom burden in recurrent and/or metastatic HNSCC.
Novel Multi-Linear Quantitative Brain Volume Formula For Manual Radiological Evaluation Of Brain Atrophy
Authors: Sungura Richard Erasto , Mpolya Emmanuel Abraham , Spitsbergen JM , Onyambu Callen Kwamboka , Sauli Elingarami , John-Mary Vianney 
Published: June 2020

Abstract
Background
The brain is a dynamic organ that develops and involutes in volume. The process of volume loss known as brain atrophy commonly occurs in elderly. However, some conditions have been implicated to provoke this paradoxical process in childhood and making it important to have methods and techniques of quantifying brain volume. Automated quantitative methods are very important in brain atrophy assessment but these tools have limited availability in developing countries. The simplified linear radiological methods are poorly reproducible and hence there is a need to develop an alternative formula that is reproducible and applicable at all healthcare levels.
Methods
The multi-linear diagonal brain fraction formula (DBF) was designed from dimensions of brain relative to skull. To test a developed formula, a total of 347 subjects aged between 0 and 18 years who had brain CT scans performed at the health facilities in Northern Tanzania were recruited and subjected to a systematic measurement of their brains in a diagonal brain fashion.
Results
Out of 347 patients evaluated, 62 subjects (17.8%) were found to be cases of brain atrophy. The three radiological measurements which included sulcal width (SW), ventricular width (VW) and Evans Index (EI) were concurrently performed. SW and VW showed good age correlation while EI showed no significant correlation with age. Similar tests were extended to diagonal brain fraction (DBF) and skull vertical horizontal ratio (VHR) in which DBF showed significant correlation.
Conclusions
The DBF formula shows significant ability of differentiating changes of brain volume suggesting that it can be utilized as an alternative brain fraction quantification method bearing technical simplicity in assessing gross brain volume with the ability to classify degrees of brain atrophy into mild, moderate, severe and very severe stages.
Transnational Migration Aspirations, Educational Capabilities, Marriage Preferences, and Health Outcomes: A Mixed-Methods Study Among Pakistani youth
Authors: Jalil Aisha , Fischer Florian 
Published: June 2020

Abstract
Background: Pakistani youths desire for international migration due to the more favourable socioeconomic circumstances in the country of destination. This may lead to migration marriages among partners of mismatching socio-economic statuses. This study aims to analyse the migration aspirations in relation with educational capabilities, marriage preferences, and health outcomes in the context of an anticipated transnational migration. Methods: A sequential mixed-methods study has been conducted. Nine case studies and a self-administered questionnaire survey among 496 students enrolled at University of Lahore, Pakistan, have been conducted. Qualitative data was analysed through thematic content analysis. Descriptive, bivariate, and multivariate analyses were performed for the quantitative data. The inductive themes in qualitative analysis, in addition to a literature review, were used to develop the structured questionnaire for the quantitative survey. Results: Significant associations of socio-demographic characteristics (gender, age, and education), migration aspirations, educational capabilities, marriage preferences, and health outcomes with the anticipated immigration status were observed. The potential migrants acquire skills and education that may support their migration plans. Conclusion: This study shows high intentions for transnational migration. Cautious policies need to be implemented for avoiding a brain drain, but also for allowing a high level of education and freedom to migrate.
Evaluation of molecular diversity analysis and relation with environmental factors in accessions of Cumin (Cuminum cyminum L.) in Iran, revealed by inter-simple sequence repeat (ISSR) markers and start codon targeted (SCoT) markers
Authors: Mohammadizadeh Mohammad Samer , Bahadori Farzaneh , Hakimi Leila , Dehshiri Abbas 
Published: June 2020

Abstract
Cuminum cyminum (as an aromatic herb) is the sources of cumin, which has been commonly used in traditional medicine for the treatment of various diseases especially in tropical Asia. Thus, the current experiment was performed to accesses molecular genetic diversity among 22 collected accessions of cumin from different area of Iran, based on inter simple sequence repeat (ISSR) markers and start codon targeted (SCoT) markers. Analysis of molecular variance revealed that a significant genetic difference between the studied accessions reach to 43%. Mantel test between genetic diversity and geographical distance indicated that an increase in geographical distance of collected accessions did not influence on genetic differentiation. Results about all agro-morphological traits showed a significant difference between the ecotypes except the number of branches. Canonical correspondence analysis ballot of genetic features and environmental factors included 5 geographic and climatic factors for seed origin’s habitat, which have been show that the mentioned parameters had no strong effect on the plant genotypes. However, difference in elevation and latitude of accession origins had effect on genetic clustering in two major groups. The consensus tree of both molecular and morphological data relieved that the accessions Chah-Dashi, Damghan, Ferdows and Nehbandan formed discreet clusters based on genetic and agro-morphological features. In conclusion, the results confirmed that both ISSR and SCoT markers were reliable and useful tools for analyzing the genetic diversity of cumin in Iran.
Clinical Characteristics and Associated factors of Trigeminal Neuralgia in Addis Ababa, Ethiopia: Experience from Addis Ababa, Ethiopia
Authors: Ayele Biniyam Alemayehu , Mengesha Abenet Tafesse , Zewde Yared Zenebe 
Published: June 2020

Abstract
Background: Trigeminal neuralgia (TN) is considered one of the most painful illnesses known to medical practice. Little is known about TN in Ethiopia. Our study aimed to assess clinical characteristics, treatment, and associated factors of TN.Method: A cross-sectional study was conducted on a total of 61 patients with confirmed Trigeminal neuralgia visiting outpatient neurology clinics of two government teaching Hospitals and two private health facilities in Addis Ababa, Ethiopia between June 2019 and March 2020.Results: Our participants' age range between 21 and 78 years with mean ± SD age of 50.7 ± 14.2 years. Males accounted for 50.8%. Twenty-five (41%) reported a prior history of one or more tooth extraction on the painful side. In the majority (68.9%) of the patient's right side of the face was affected. The mandibular nerve was the commonly involved branch (47.5%). Fifty-five (90.2%) of patients fulfilled criteria for classical TN and 9.8% had symptomatic TN. The majority of the participants reported mixed types of pain such as burning, lancinating, and electric shock-like. Well defined trigger zone was identified in one-third (36%) of cases. Carbamazepine was the most commonly prescribed drug with a median dose of 600mg (IQR: 400 – 1000mg). Two-third of the patients reported prominent satisfaction. The mean (± SD) dose of carbamazepine used to control the pain was significantly higher among those with dental extraction history as compared to those with no history of dental extraction (736 ± 478.6mg Vs 661.1 ± 360.4mg, respectively, T = -2.06, p = 0.04 95% CI:-213.41 to -2.98). A statistically significant number of patients who had single branch involvement reported prominent satisfaction with their treatment as compared to those who had more than one branch involvement. (95% CI: 1.3-3.8: p = 0.006). Conclusion: The majority of our patients had Classical TN in the mandibular nerve distribution on the right side of the face and well satisfied with carbamazepine only treatment. Furthermore, we observed a higher proportion of dental extraction among our patients, hinting at the scale of miss and delayed-diagnoses. Thus, we recommend conducting a well-designed prospective study to support our findings.
Effect of Advanced Chelate Technology Based Trace Minerals on Growth Performance, Mineral Digestibility, Tibia Characteristics, and Antioxidant Status in Broiler Chickens
Authors: Ghasemi Hossein Ali , Hajkhodadadi Iman , Hafizi Maryam , Taherpour Kamran , Nazaran Mohammad Hassan 
Published: June 2020

Abstract
Background: Compared to the corresponding source of inorganic trace minerals (TM), chelated supplements arecharacterized by better physical heterogeneity and chemical stability and appear to be better absorbed in the gut due to possiblydecreased interaction with other feed components.Methods:This study was designedin broiler chickens to determine theeffects ofreplacing inorganic trace minerals (TM) with an advanced chelate technology based supplement(Bonzachicken) on growth performance, mineral digestibility, tibia bone quality, and antioxidant status. A total of 625 male 1-d-old broiler chickens were allocated to 25 pens and assigned to 5 dietary treatments in a completely randomized design.Chelated TM (CTM) supplement was compared at 3 levels to no TM(NTM) or inorganic TM. A corn–soy-based control diet was supplemented with inorganic TMat the commercially recommended levels (ITM), i.e., iron, zinc, manganese, copper, selenium, iodine, and chromium at 80, 92, 100, 16, 0.3, 1.2, and 0.1 mg/kg, respectively, and varying concentration of CTM, i.e., match to 25, 50, and 100 % of the ITM (diets CTM25, CTM50, and CTM100, respectively).Results: All diets, except diet CTM25, increased average daily gain (ADG), European performance index (EPI), and serum total antioxidant capacity compared to the NTM diet (P < 0.05). Broilers fed the CTM100 diet had lowest overall FCR and highest BWG, EPI, tibia ash, zinc, and manganese contents (P < 0.05). The tibia phosphorus content and apparent ileal digestibilities of phosphorus, zinc, and manganese were lower in the ITM group compared with the CTM50 and CTM100 groups (P < 0.05). Broiler chickens fed any of the diets exhibited higher serum glutathione peroxidase and superoxide dismutase activities and lower malondialdehyde level than those fed the NTM diet, where the best values were found for CTM100 treatment (P < 0.05).Conclusions: These results indicatethat while CTMsupplementation to 25 and 50% of the commercially recommended levels could support growth performance, a totally replacing ITM by equivalent levels of CTM could also improve growth performance, bone mineralization and antioxidant status of broiler chickens under the conditions of this study.
Validity of Anthropometric Cut-offs for Early Diagnosis of Dyslipidemia among Adults in Jimma Town, Southwest Ethiopia
Authors: Kenate Sileshi , Tesfaye Temamen , Berhanu Solomon , Zawdie Belay , Tesfaye Yonas , Sena Lelisa , Tadesse Mulualem , Kebede Ayantu , Kebede Esayas , Tamiru Dessalegn 
Published: June 2020

Abstract
Background: Lack of regional and local based cut off points of lipid profile and/or anthropometric measurement remains one of the challenges in prevention, early detection and control of non-communicable diseases. This study was aimed to validate anthropometric based screening of lipid profile in order to prevent potential predictors of major non-communicable diseases.Methods: Community based cross sectional study was conducted among randomly selected 977 adults in Jimma Town, Ethiopia from July 20 to August 20, 2019. Data were collected using structured questionnaire, anthropometric and biochemical measurements. Data were analyzed using SPSS windows version 20 and Kappa statistic (K) was used to validate the agreement between anthropometric measurement and lipid profile of the study participants. A p-value of < 0.05 was considered statistically significant.Results: Body mass index (BMI) at ≥24.5 was used as screening of dyslipidemia (TG≥150mg/dl) with slight Kappa coefficient of 0.138 (P<0.001) among females while it was ≥22.2 among males with fair (0.275) Kappa coefficient (P<0.001). Waist circumference based screening of dyslipidemia (TG≥ 150mg/dl) at ≥78.0cm had negative (-0.005) Kappa coefficient (P<0.001) among females (sensitivity: 72.6% & specificity: 26.7%). Yet, waist circumference at ≥83.7cm had slight Kappa coefficient of 0.13(P<0.005) among males (sensitivity: 38% & specificity: 74.9%). Waist hip ratio based screening of dyslipidemia (TG≥150mg/dl) at ≥0.82 among females had negative (-0.001) Kappa coefficient (p=0.763) whereas among males at ≥0.88 there was a slight (0.105) Kappa coefficient of (p=0.002) (Sensitivity: 77.5% & Specificity: 36.8%). This study showed that anthropometric based of high-density lipoprotein measurement was not applicable. Conclusions: Findings of this study indicated that BMI-based screening of triglyceride was applicable for both sexes than other anthropometric measurements. Waist circumference and Waist to hip ratio-based screening of triglyceride was slightly applicable only for males. However, anthropometric based screening of high-density lipoprotein measurement was not applicable for both sexes. In conclusion, researchers and policy makers need to consider local cut off points to conduct screening nutritional status of the community.