bluuebunny/crossref_metadata_2025_split huggingface dataset:
SPATIO‐TEMPORAL VARIATIONS OF SEVEN WEATHER VARIABLES IN IRAN: APPLICATION OF CRU TS AND GPCC DATA SETS†
Authors: Ababaei Behnam 
Published: January 2020

AbstractIran's climate‐sensitive agriculture and water resources are vulnerable to climate change and investigation of climatic trends helps in preparing adaptation strategies. Weather stations are sparsely distributed and access to complete weather data is limited. In such situations, gridded global/regional data sets are promising alternatives. Here, monthly time series of seven weather variables (i.e. monthly averages or monthly totals of daily values) were obtained from the Climatic Research Unit TS V4.01 and Global Precipitation Climatology Centre V7 gridded data sets in 675 grid cells covering the country and analysed over the periods 1957–1986 and 1987–2016 at annual, seasonal and monthly scales. Over the two periods and at a national scale, mean temperature has increased by 0.004 (P = 0.717) and 0.04 °C yr−1 (P = 0.000), while the diurnal temperature range has not significantly changed (P > 0.6). Annual total precipitation experienced an insignificant increase (0.81 mm yr−1; P = 0.666) over the first period but declined by 2.12 mm yr−1 (P = 0.041) over the second. Potential evapotranspiration (PET) has increased by 0.32 (P = 0.398) and 1.43 mm yr−1 (P = 0.015), respectively. Since 1987, significant increasing trends in temperature were detected all over the country. While significant increasing trends in annual precipitation were detected in the central regions and south‐west over the first period, decreasing trends prevailed during 1987–2016 in the south, southwest and east with winter being the largest contributor to annual trends. Over the last three decades, annual PET has increased mostly in the north‐west and south‐east while significant increasing trends were detected in 89% of grid cells, except in a few cells in the north‐east. Cloud cover, vapour pressure and frequency of frost days were also analysed. These results are crucial for policy‐makers, researchers and engineers in the country and internationally who usually base their decisions and designs on outdated data sparsely distributed in space. © 2020 John Wiley & Sons, Ltd.
Retracted: The promoting effect of MMP13 on mediating the development of HFLS‐RA by the target of miR‐19a through IL‐17 signaling pathway
Authors: Su Jiang , Zhang Jing , Zhu Jing , Liu Yi 
Published: January 2020

AbstractBy investigating the expression profiles of miR‐19a and metalloproteinases (MMP13) in human fibroblast‐like synoviocytes‐rheumatoid arthritis (HFLS‐RA) and HFL cells lines, this study intends to confirm the directly target connection between them and reveal the effect of suppressing MMP13 on HLFS‐RA migration, invasion and apoptosis. After screening the abnormal expressed messenger RNAs and microRNAs in synovial tissues of patients with RA, the underlying pathway was determined by Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. The HFLS‐RA cell line was transfected for the following experiments with pcDNA3.1(+) served as vector. The directly target association between miR‐19a and MMP13 was confirmed by Luciferase reporter assay. Microarray analysis suggested that MMP13 was upregulated while miR‐19a was downregulated in HFLS of RA tissues compared with the healthy control group. MMP13 was related to many proteins in protein‐protein interaction network, which might be the main influencing factor of RA. KEGG pathway analysis identified that interleukin (IL)‐17 pathway was activated in the regulation of MMP13 in the development of RA. Through observing the alteration of luciferase activity, miR‐19a could indeed bind to the 3′UTR of the downstream of MMP13, the target association was then confirmed. The proliferation and invasion of HFLS‐RA were promoted by overexpressing MMP13 protein. miR‐19a could function as a suppressor of MMP13 and thereby retard the severity of RA. The results showed that miR‐19a could regulate the expression of MMP13 in HFLS‐RA by mediating the proliferation and invasion of HFLS‐RA through IL‐17 signaling pathway, thereby participating in the degradation of chondrocytes in the progression of RA.
Circular RNA circABCC4 regulates lung adenocarcinoma progression via miR‐3186‐3p/TNRC6B axis
Authors: Liu Meijuan , Wang Pengwei , Sui Xiaomei , Ding Fang , Liu Li , Gao Zengyan , Cheng Zhaozhong 
Published: January 2020

AbstractLung adenocarcinoma (LUAD), a general kind of bronchogenic malignancy globally, is depicted as one of the most critical factors affecting human health severely. Featured with loop structure, circular RNA (circRNA) has been described as an essential regulator of multiple human malignancies. Nevertheless, knowledge concerning the regulatory function of circRNA in LUAD progression remains limited. Identified as a novel circRNA, circABCC4 has not been studied in LUAD as yet. This is the first time to probe into the underlying role of circABCC4 in LUAD. In this study, a notably elevated expression of circABCC4 was found in LUAD tissues and cells. Besides, circABCC4 is verified to be characterized with a circular structure in LUAD. Functional assays elucidated that knockdown of circABCC4 significantly impaired LUAD cell proliferation, migration as well as accelerated cell apoptosis. Molecular mechanism experiments later revealed that circABCC4 could bind with miR‐3186‐3p and miR‐3186‐3p was a tumor suppressor in LUAD. Moreover, TNRC6B was validated to combine with miR‐3186‐3p, and its expression was respectively negatively and positively regulated by miR‐3186‐3p and circABCC4 in LUAD. Final rescue experiments further delineated that TNRC6B upregulation partially restored circABCC4 downregulation‐mediated effect on LUAD progression. In sum, circABCC4 regulates LUAD progression via miR‐3186‐3p/TNRC6B axis.
Exosomes from mesenchymal stem cells overexpressing MIF enhance myocardial repair
Authors: Liu Xiaolin , Li Xin , Zhu Wenwu , Zhang Yuelin , Hong Yimei , Liang Xiaoting , Fan Baohan , Zhao Hongyan , He Haiwei , Zhang Fengxiang 
Published: January 2020

AbstractAccumulating evidence has shown that mesenchymal stem cell (MSC)‐derived exosomes (exo) mediate cardiac repair following myocardial infarction (MI). Macrophage migration inhibitory factor (MIF), a proinflammatory cytokine, plays a critical role in regulating cell homeostasis. This study aimed to investigate the cardioprotective effects of exo secreted from bone marrow‐MSCs (BM‐MSCs) overexpressing MIF in a rat model of MI. MIF plasmid was transducted in BM‐MSCs. Exo were isolated from the supernatants of BM‐MSCs and MIF‐BM‐MSCs, respectively. The morphology of mitochondria in neonatal mice cardiomyocytes (NRCMs) was determined by MitoTracker staining. The apoptosis of NRCMs was examined by deoxynucleotidyl transferase‐mediated dUTP nick end‐labeling. BM‐MSC‐exo and MIF‐BM‐MSC‐exo were intramuscularly injected into the peri‐infarct region in a rat model of MI. The heart function of rats was assessed by echocardiography. The expression of MIF was greatly enhanced in MIF‐BM‐MSCs compared with BM‐MSCs. Both BM‐MSC‐exo and MIF‐BM‐MSC‐exo expressed CD63 and CD81. NRCMs treated with MIF‐BM‐MSC‐exo exhibited less mitochondrial fragmentation and cell apoptosis under hypoxia/serum deprivation (H/SD) challenge than those treated with BM‐MSC‐exo via activating adenosine 5′‐monophosphate‐activated protein kinase signaling. Moreover, these effects were partially abrogated by Compound C. Injection of BM‐MSC‐exo or MIF‐BM‐MSC‐exo greatly restored heart function in a rat model of MI. Compared with BM‐MSC‐exo, injection of MIF‐BM‐MSC‐exo was associated with enhanced heart function, reduced heart remodeling, less cardiomyocyte mitochondrial fragmentation, reactive oxygen species generation, and apoptosis. Our study reveals a new mechanism of MIF‐BM‐MSC‐exo‐based therapy for MI and provides a novel strategy for cardiovascular disease treatment.
miR‐885 mediated cardioprotection against hypoxia/reoxygenation‐induced apoptosis in human cardiomyocytes via inhibition of PTEN and BCL2L11 and modulation of AKT/mTOR signaling
Authors: Meng Xin , Mei Lijun , Zhao Chedong , Chen Wei , Zhang Ning 
Published: January 2020

AbstractIschemia/reperfusion (I/R) injury could cause the enhanced cell apoptosis of cardiomyocytes, which is one of key contributors for the development of ischemic heart disease. Recent studies emphasized the role of microRNAs (miRNAs) in regulating cardiomyocyte apoptosis. The study planned to elucidate the molecular actions of miR‐885 on mediating human cardiomyocytes (HCMs) apoptosis induced by hypoxia/reoxygenation (H/R) and to explore the potential molecular mechanisms. The present data revealed that H/R stimulation inhibited HCM viability and potentiated HCM apoptosis, and more importantly, the expression of miR‐885 in HCMs was markedly repressed after H/R stimulation. Further experimental examinations demonstrated that overexpression of miR‐885 attenuated H/R‐induced increased in HCM apoptotic rates, while miR‐885 knockdown impaired HCM viability and increased HCM apoptotic rates. Moreover, the mechanistic studies showed that miR‐885 inversely regulated the expression of phosphatase and tensin homolog (PTEN) and BCL2 like 11 (BCL2L11) in HCMs, and enforced expression of PTEN and BCL2L11 partially antagonized the protective actions of miR‐885 overexpression on H/R‐induced HCM injury. Moreover, H/R suppressed AKT/mTOR signaling, which was attenuated by miR‐885 overexpression in HCMs. In conclusion, the present study for the first time showed the downregulation of miR‐885 induced by H/R in HCMs, and provided the evidence that miR‐885 attenuated H/R‐induced cell apoptosis via inhibiting PTEN and BLC2L11 and modulation of AKT/mTOR signaling in HCMs.
Circular RNA hsa_circ_0004277 contributes to malignant phenotype of colorectal cancer by sponging miR‐512‐5p to upregulate the expression of PTMA
Authors: Yang Lu , Sun Hongwei , Liu Xing , Chen Jinxin , Tian Zhimin , Xu Jia , Xiang Bowen , Qin Baoli 
Published: January 2020

AbstractIn recent years, extensive reports have been published concerning the molecular mechanism underlying the occurrence and progression of colorectal cancer. Circular RNAs (circRNAs) have been identified as important modulators in the biological processes of colorectal cancer. Microarray analysis unveiled that differential circ‐0004277 expression was identified in tissue samples of colorectal cancer. High circ‐0004277 expression was then verified in tissue samples and cell lines of colorectal cancer via qRT‐PCR. Kaplan–Meier analysis was used for identifying the association between circ‐0004277 expression and the overall survival rate of colorectal cancer patients. A relationship existed between higher circ‐0004277 expression and decreased overall survival rate of colorectal cancer patients. From a functional perspective, circ‐0004277 knockdown accelerated cell apoptosis and restrained cell proliferation of colorectal cancer. From mechanistic perspective, circ‐0004277 upregulated PTMA by sponging miR‐512‐5p. Rescue assay was used for verifying the roles of the circ‐0004277‐miR‐512‐5p‐PTMA axis. Both miR‐512‐5p and PTMA participated in circ‐0004277‐mediated colorectal cancer cell proliferation based on experiments. In summary, our study showed that circ‐0004277 promoted the proliferation of colorectal cancer cells as a miR‐512‐5p sponge to upregulate the PTMA expression.
Wnt/β‐catenin‐mediated heat exposure inhibits intestinal epithelial cell proliferation and stem cell expansion through endoplasmic reticulum stress
Authors: Zhou Jia‐yi , Huang Deng‐gui , Zhu Min , Gao Chun‐qi , Yan Hui‐chao , Li Xiang‐guang , Wang Xiu‐qi 
Published: January 2020

AbstractHeat stress induced by continuous high ambient temperatures or strenuous exercise in humans and animals leads to intestinal epithelial damage through the induction of intracellular stress response. However, the precise mechanisms involved in the regulation of intestinal epithelial cell injury, especially intestinal stem cells (ISCs), remain unclear. Thereby, in vitro a confluent monolayer of IPEC‐J2 cells was exposed to the high temperatures (39, 40, and 41°C), the IPEC‐J2 cell proliferation, apoptosis, differentiation, and barrier were determined, as well as the expression of GRP78, which is a marker protein of endoplasmic reticulum stress (ERS). The Wnt/β‐catenin pathway‐mediated regenerative response was validated using R‐spondin 1 (Rspo1). And ex‐vivo, three‐dimensional cultured enteroids were developed from piglet jejunal crypt and employed to assess the ISC activity under heat exposure. The results showed that exposure to 41°C for 72 hr, rather than 39°C and 40°C, decreased IPEC‐J2 cell viability, inhibited cell proliferation and differentiation, induced ERS and cell apoptosis, damaged barrier function and restricted the Wnt/β‐catenin pathway. Nevertheless, Wnt/β‐catenin reactivation via Rspo1 protects the intestinal epithelium from heat exposure‐induced injury. Furthermore, exposure to 41°C for 24 hr reduced ISC activity, stimulated crypt‐cell apoptosis, upregulated the expression of GRP78 and caspase‐3, and downregulated the expression of β‐catenin, Lgr5, Bmi1, Ki67, KRT20, ZO‐1, occludin, and claudin‐1. Taken together, we conclude that heat exposure induces ERS and downregulates the Wnt/β‐catenin signaling pathway to disrupt epithelial integrity by inhibiting the intestinal epithelial cell proliferation and stem cell expansion.
MicroRNA‐214‐3p enhances erastin‐induced ferroptosis by targeting ATF4 in hepatoma cells
Authors: Bai Tao , Liang Ruopeng , Zhu Rongtao , Wang Weijie , Zhou Lin , Sun Yuling 
Published: January 2020

AbstractPrimary liver cancer is the second most frequent cause of cancer‐related deaths. Ferroptosis, a recognized form of regulated cell death, recently gains attention. MicroRNA‐214‐3p (miR‐214) plays a regulatory role in hepatocarcinogenesis. However, the role of miR‐214 in cellular ferroptosis is unclear. This study aimed at elucidating whether miR‐214 could regulate ferroptosis of liver cancer. In vitro, HepG2 and Hep3B cancer cells were treated with erastin, a ferroptosis inducer, and then erastin was demonstrated to suppress the cell viability. Moreover, pre‐miR‐214 overexpression caused that HepG2 and Hep3B cells were more susceptible to erastin, whereas anti‐miR‐214 sponge showed the opposite effect. Additionally, pre‐miR‐214 overexpression increased the malondialdehyde and reactive oxygen species levels, upregulated Fe2+ concentration, and decreased glutathione levels in cancer cells exposed to erastin. Further, erastin enhanced the activation of transcription factor 4 (ATF4) in HepG2 and Hep3B cells, and pre‐miR‐214 overexpression inhibited ATF4 expression. The luciferase reporter data validated ATF4 as a direct target of miR‐214. Cancer cells transfected with ATF4 overexpression plasmid rendered lower susceptible to miR‐214‐induced ferroptotic death. In vivo, erastin significantly reduced the size and weight of xenografted tumors, and miR‐214 elevated the ferroptosis‐promoting effects of erastin and decreased ATF4 expression. In summary, our study demonstrates that the ferroptosis‐promoting effects of miR‐214 in hepatoma cells are attributed at least to its inhibitory effects on ATF4, which may provide a new target for therapy of hepatoma regarding ferroptosis.
Design, synthesis, and antimicrobial evaluation of some nifuroxazide analogs against nosocomial infection
Authors: Dwivedi Deepak K. , Sahu Adarsh , Dighade Sachin J. , Agrawal Ram Kishore 
Published: January 2020

AbstractA series of 10 p‐substitutedbenzoylmethylene hydrazide derivatives 4a‐j were synthesized by protecting carboxylic group of 4‐hydroxybenzoic acid using methanol and sulfuric acid than reacting it with hydrazide to form 4‐hydroxybenzohydrazide followed by reacting with a variety of aldehydes and evaluated for their activity against nosocomial infection. All the synthesized compounds were characterized by Fourier‐transform infrared (FT‐IR), 1H nuclear magnetic resonance (NMR), and mass spectral data. The in vitro antimicrobial potential of synthesized compounds was estimated against prominent strains of nosocomial pathogens (Staphylococcus aureus, Escherichia coli, and Aspergillus niger). The antimicrobial evaluation revealed compounds 4b, 4c, 4d, 4e, 4f, and 4j to be the most active compounds of the series with IC50 value for antibacterial in the range 0.39 to 0.75 μM/mL. Furthermore, the in vitro cytotoxic potential of the compounds was appraised by hemolytic assay. The results showed that some of the synthesized compounds exhibited marked activity.
Sensory and nutritional properties of peanut‐based beverages: a promising solution for undernutrition in Malawi and possibly beyond
Authors: Gama Aggrey P , Mwangwela Agnes M , Gichohi‐Wainaina Wanjiku N , Adhikari Koushik 
Published: January 2020

AbstractBACKGROUNDUndernutrition remains a challenge in countries like Malawi. The search for effective nutrition interventions to address this challenge is ongoing. In this study, the sensory profiles of six peanut‐based beverage prototypes were determined. Three of the prototypes had barley malt in their formulations, and the other three had sorghum malt in their formulations. Nutrient profiles were determined using official standard analytical methods, and the results were compared with daily nutrient requirements to estimate their nutritional value.RESULTSAll the six prototypes were acceptable, with mean overall liking scores ranging from 7.0 to 7.6. Prototypes containing sorghum malt were perceived as tasty, creamy, thick, and whitish, whereas those containing barley malt were perceived as sweet, watery, and brown. These terms, except whitish, had a significant impact (P < 0.05) on the overall liking mean score. The formulation incorporating sorghum malt (F‐B) had a better nutrient profile than that containing barley malt (F‐A) in terms of protein, total dietary fiber, potassium, phosphorus, calcium, magnesium, iron, and zinc. A 237 mL (8 oz.) serving of F‐B was an excellent source (% DV ≥20) of high‐quality protein, total dietary fiber, phosphorus, calcium, molybdenum, and manganese, and a good source (10 ≤ % DV < 20) of potassium and magnesium.CONCLUSIONBased on consumer acceptability, both formulations can potentially increase peanut consumption in Malawi. Given its nutritional quality, F‐B may help in the management of undernutrition. The peanut‐based beverage may also be valuable in other countries, besides Malawi, where undernutrition is also a challenge. © 2020 Society of Chemical Industry
Theoretical Aspects of Iterative Coupling for Linear Oligomers and Polymers
Authors: Saker Neto Nicolau , Jones David John , Wong Wallace Wing Ho 
Published: January 2020

AbstractA conceptual study of iterative coupling (IC) is performed, providing a unified description and new research directions. IC chain growth rates and functional group choice are analyzed, guiding construction of efficient schemes. The concept of cycle efficiency is defined as a more complete metric of experimental implementations of IC, and then applied to the main linear and exponential IC processes. The mathematical relations between individual reactions, cycles, and the iterative process as a whole are studied. Finally, macromolecule IC is proposed as a strikingly complementary process to standard IC, with potential to reduce the dispersity of non‐uniform samples. Due to its connection with the central limit theorem of statistics, it provides an unusually robust, powerful, and general method for scalable production of polymer samples with narrow distribution. In all, this contribution assists development of improved IC processes targeting low dispersity linear oligomers and polymers.
Compact dual‐band bandpass filter using a modified hexagonal split ring resonator
Authors: Troudi Zied , Macháč Jan , Osman Lotfi 
Published: January 2020

AbstractIn this article, a compact dual‐band bandpass filter (BPF) is studied and designed by utilizing a modified hexagonal split ring resonator (H‐SRR). The modified H‐SRR consists of a classical H‐SRR loaded by an open‐stepped impedance stub and a grounding via located at the symmetrical plane of the resonator. The proposed BPF operates at 2.5 and 4.83 GHz. Its advantages include simplicity of design, high rejection level due to transmission zeros, and low insertion loss. In addition, the size of the circuit occupies only 142.6 mm2. A good agreement is obtained between measurement and simulation results.
Can multicentre urodynamic studies provide high quality evidence for the clinical effectiveness of urodynamics? ICI‐RS 2019
Authors: Rademakers Kevin , Gammie Andrew , Yasmin Habiba , Cardozo Linda , Greenwell Tamsin , Harding Christopher , Kirschner‐Hermanns Ruth , Marcelissen Tom , Finazzi‐Agro Enrico 
Published: January 2020

AbstractAimsLower urinary tract (LUT) function can be investigated by urodynamic studies (UDS) to establish underlying functional abnormalities in the LUT. A multicentre registry could present an opportunity to improve the scientific evidence base for UDS. During the International Consultation on Incontinence Research Society (ICI‐RS) meeting in Bristol, United Kingdom 2019, an expert panel discussed the potential of a multicentre urodynamic registry to improve the quality of urodynamic output.Methodsthe potential importance of a multicentre urodynamic registry, parameter inclusion, quality control, and pitfalls during a registry roll‐out were reviewed and discussed.Results and ConclusionsThe clinical utility, evaluation, and effectiveness of UDS remain poorly defined due to a lack of high quality evidence and large study populations. Therefore, the ICI‐RS proposes formation of a urodynamic panel for future roll‐out of a registry. The inclusion of basic parameters was discussed and the essential parameters were defined as well as the potential pitfalls of a registry roll‐out. The discussion and recommendations in this paper form the base for future urodynamic registry development.