bluuebunny/crossref_metadata_2025_split huggingface dataset:
KDM4 Orchestrates Epigenomic Remodeling of Senescent Cells and Potentiates the Senescence-Associated Secretory Phenotype
Authors: Zhang Boyi , Long Qilai , Wu Shanshan , Song Shuling , Xu Qixia , Han Liu , Qian Min , Ren Xiaohui , Jiang Jing , Fu Qiang , Guo Jianming , Zhang Xiaoling , Chang Xing , Lam Eric , Campisi Judith , Kirkland James , Sun Yu 
Published: August 2020

Abstract
Cellular senescence restrains the expansion of neoplastic cells through several layers of regulation, including epigenetic decoration of chromatin structure and functional modulation of bioactive components. Here we report that expression of the histone H3-specific demethylase KDM4 is upregulated in human stromal cells upon cellular senescence. In clinical oncology, upregulated KDM4 and diminished H3K9/H3K36 methylation are correlated with adverse survival of cancer patients post-chemotherapy. Global chromatin accessibility mapping via ATAC-seq and expression profiling through RNA-seq reveal extensive reorganization of chromosomes and spatiotemporal reprogramming of the transcriptomic landscape, events responsible for development of the senescence-associated secretory phenotype (SASP). Selectively targeting KDM4 dampens the SASP of senescent stromal cells and enhances the apoptotic index of cancer cells in the treatment-damaged tumor microenvironment (TME), together prolonging overall survival of experimental animals. Our study supports the dynamic change of H3K9/H3K36 methylation marks during cellular senescence, identifies an unusually permissive chromatin state, unmasks KDM4 as a key modulator of the SASP, and presents a novel therapeutic avenue to manipulate cellular senescence and curtail age-related pathologies.
Epidemiology of Injury Among School-Going Adolescents in Ghana: Evidence From the Global School-Based Students Health Survey
Authors: Essien Samuel Kwaku , Chireh Batholomew , Melese Kidest Getu 
Published: August 2020

Abstract
Background: Unintentional injuries are a serious global public health problem and the second leading cause of death among adolescents age between 10-19 years. It is also the least researched in most low-income countries and Ghana in particular. This study aims to provide estimates of the prevalence, causes, and associated risk factors of unintentional injuries among school-going adolescents in Ghana.Methods: This is a cross-sectional study using data from the Ghana Global School-based Student Health Survey conducted in 2012 (N=3632). A two-stage cluster probability sampling technique was employed to select a representative sample of Junior and Senior High School students in ten administrative regions of the country. Cross tabulations and chi-square tests were used to provide estimates of prevalence, frequency, and causes of injuries. In the multivariable stage, a multiple logistic regression based on SAS 9.4 survey logistic modeling procedure was conducted to examine the association between unintentional injuries with explanatory variables. The final adjusted model was assessed using a significant level of (p<0.05).Results: In this study, 40.96% (1488) of school-aged adolescents reported having experienced an unintentional injury. Of these injuries, 64.5% occurred at home, while the least occurred via traffic (15.0%). The frequency of injury did not differ between boys and girls (P=0.368), but significantly higher in adolescents aged ≤17 than in those ≥ 18 years (P<0.001). Also, injuries were significantly higher in junior high school than in senior high school adolescents. Age, physical activity, sleeping problems, and loneliness were found to be the significant risk factors associated with unintentional injury among adolescents.Conclusion: Prevalence of unintentional injuries were widespread among adolescents in Ghana. Prompt prevention of these injuries among adolescents should be a public health priority. Preventive strategies in schools and homes should be enhanced. Injury preventive measures should emphasize on adolescents. Treatment options should be made available to adolescents struggling with a sleeping problem, loneliness, and those with accidents in physical activity.
Possible associations between callers’ degree-of-worry and their socioeconomic status when contacting out-of-hours services: a prospective cohort study
Authors: Gamst-Jensen Hejdi , Thilsted Sita LeBlanc , Folke Fredrik , Tolstrup Janne S. , Thygesen Lau Caspar 
Published: August 2020

Abstract
Background:Telephone triage within out-of-hours (OOH) services aims to ascertain the urgency of a caller’s medical condition in order to determine the correct type of health care needed, ensuring patient safety. To improve the triage process by increasing patient‐centred communication, a triage tool has been developed, whereby callers are asked to rate their degree-of-worry (DOW) as a measure of self-evaluated urgency. Studies show that low socioeconomic status (SES), being single and non-Western ethnicity are associated to low self-rated health and morbidity and these factors may also be associated with high DOW. The aim of this paper was to examine if low SES, being single and non-Western ethnicity were associated to high DOW of callers contacting OOH services. Methods: A prospective cohort study design, at the OOH services for the Capital Region of Denmark. Over two weeks, 6,869 of 38,787 callers met the inclusion criteria: ³18 years, patients themselves or close relative/friend, reported DOW, had a valid personal identification number and gave informed consent. Callers were asked to report their DOW (1=minimal worry to 5=maximal worry), which was dichotomized into low (1-3) and high (4-5) DOW and linked to data from electronical medical records and Statistics Denmark. Socioeconomic factors (education and annual household income), marital status and ethnicity were assessed in relation to DOW by logistic regression.Results: High DOW was reported by 38.2% of the participants. Low SES (low educational level; OR 1.5, 95% CI 1.3-1.7 and low annual household income; 1.5, 1.3-1.6) was associated with high DOW and so too was being single; 1.2, 1.1-1.3 and of non-Western ethnicity; 2.9, 2.5-3.4.Conclusions: Knowledge of the association of low SES, marital status as single and non-Western ethnicity with high DOW among callers to OOH services may give call handlers a better understanding of callers’ DOW. If this does not correspond to the call handler’s perception of urgency, this knowledge may further encourage patient-centred communication, aid the triage process and increase patient safety. A better understanding of socioeconomic variables and their relation to callers’ DOW gives direction for future research to improve telephone triage of OOH services.
Employment anxiety and its relationship with student nurse interns’ social support and job-seeking efficacy : a path analysis
Authors: feng Yi qi , Zhang Cuijiao , Yang Guoli , Qi Mengying , Kaminga Atipatsa , Huang Hui , Yan Jin 
Published: August 2020

Abstract
Purpose

This study was intended to explore the relationship of employment anxiety with student nurse interns’ social support and job-seeking efficacy.
Method:

This was a cross-sectional study conducted from three tertiary hospitals in Hunan Province, China.Student nurse interns were recruited conveniently from March to May 2020, to complete a questionnaire, which included demographic information, occupation anxiety of college students, job-seeking efficacy of college students, and social support scale. Descriptive statistics were used to summarize sample characteristics, while Pearson's correlation analysis was used to explore the relationship specified in the purpose of this study. Multiple linear regression analysis was used to determine factors that influenced employment anxiety of student nurse interns, and then these factors were included in a path analysis model.
Results

A total of 560 student nurse interns were issued questionnaires but only 534 questionnaires were completed. Finally, 494 valid questionnaires were included for analysis. The average ± SD total score of employment anxiety was 71.49±17.28, where SD denotes standard deviation. Job-seeking efficacy, social support, priority factors for finding a job and having received employment training had both direct and indirect impact on employment anxiety. Social support and having received employment training imposed indirect influence on employment anxiety by the intermediate variable of job-seeking efficacy.
Conclusion

The results showed that student nurse interns had a higher level of employment anxiety, which was mostly influenced by job-seeking efficacy followed by social support, and then priority factors for finding a job. Therefore, these results may suggest that nurse educators and managers should consider in strengthening the provision of social and psychological support to student nurse interns, in order to improve their job-seeking efficacy so as to alleviate their employment anxiety.
Advantages and Disadvantages of His-Tagged Beta-Galactosidase
Authors: Flores Sandra S. , Clop Pedro D. , Barra José L. , Argaraña Carlos , Perillo María A. , Nolan Verónica , Sanchez Julieta M. 
Published: August 2020

Abstract
β-Galactosidase is one of the most important biotechnological enzyme used in the dairy industry, pharmacology and in molecular biology. In our laboratory we have overexpressed a recombinant β-galactosidase in Escherichia coli (E. coli). This enzyme differs from its native version (β-GalWT) in that 6 histidine residues have been added to the carboxyl terminus in the primary sequence (β-GalHis), which allows its purification by immobilized metal affinity chromatography (IMAC). In this work we compared the functionality and structure of both proteins and evaluated their catalytic behavior on the kinetics of lactose hydrolysis. We observed a significant reduction in the enzymatic activity of β-GalHis with respect to β-GalWT. Although, both enzymes showed a similar catalytic profile as a function of temperature, β-GalHis presented a higher resistance to the thermal inactivation and evidenced greater half-life time compared to β-GalWT. At room temperature, β-GalHis showed a fluorescence spectrum compatible with a partially unstructured protein however, it exhibited a lower tendency to the thermal-induced unfolding with respect to β-GalWT. Analytical ultracentrifugation experiments demonstrated that the population of β-GalHis molecules exhibited a higher proportion of monomers and a lower proportion of tetrameric species with respect to the His-tag free protein. The impairment of tetramerization may would explain the negative effect of the presence of His-tag on the enzymatic activity. In addition, the present results, analyzed in the context of the available literature, suggest that the effect of the His-tag is protein-specific.
Blue LED causes cell death in human hepatoma by inducing DNA damage
Authors: He Mingyu , Li Guanghui , He Xiaoqi , Wang Yang , Lei Hong , Wang Quan , Yan Gege , Gong Rui , Liu Guoxin , Li Tao , Cai Benzhi , Li Linqiang , Yuan Ye 
Published: August 2020

Abstract
Phototherapies, including sunlight, infrared, ultraviolet, visible and laser, parts of which present high curative effect, small invasion, and negligible adverse reactions in cancer treatment. Here we aimed to explore the potential therapeutical effects of blue LED in hepatoma cell and decipher the underlying cellular/molecular mechanisms. We demonstrated that the irradiation of blue LED light in hepatoma cell could lead to cell proliferation reduction along with the cell apoptosis increase. Simultaneously, blue LED irradiation also markedly suppressed the migration and invasion ability of hepatoma cells. Sphere formation analysis further revealed the decreased stemness of hepatoma cell under the treatment of blue LED irradiation. In addition, blue LED irradiation significantly promoted the expression of γ-H2AX, a sensitive molecular marker of DNA damage. Collectively, we demonstrated that blue LED irradiation exhibited anti-tumor effects on liver cancer by inducing DNA damage, representing a potential approach for human hepatoma treatment.
Validation of a QTL for Grain Size and Weight Using an Introgression Line from a Cross Between Oryza Sativa and Oryza Minuta
Authors: Feng Yue , Yuan Xiaoping , Wang Yiping , Yang Yaolong , Zhang Mengchen , Yu Hanyong , Xu Qun , Wang Shan , Niu Xiaojun , Wei Xinghua 
Published: August 2020

Abstract
Background: Grain size and weight are important target traits determining grain yield and quality in rice. Wild rice species possess substantial elite genes that can be served as an important resource for genetic improvement of rice. In this study, we identify and validate a novel QTL on chromosome 7 affecting the grain size and weight using introgression lines from cross of Oryza sativa and Oryza minuta.Results: An introgression line ‘IL188’ has been achieved from a wild species Oryza minuta (2n = 48, BBCC, W303) into O. sativa japonica Nipponbare. The F2 and F2:3 populations derived from a cross between IL188 and Nipponbare were used to map QTLs for five grain size traits. A total of 12 QTLs for the five grain traits were identified on chromosomes 1, 2, 3, 6, 7, and 8. The QTL qGL7 on chromosome 7 was detected stably in the F2 and F2:3 populations, and explained 15.09-16.30% of the phenotypic variance. To validate the effect of qGL7, eight residual heterozygous line (RHL) populations were developed through selfing four F2:3 and four F2:4 plants with different heterozygous segments for the target region. By further developing SSR and Indel markers in the target interval, qGL7 was delimited to a ~261 kb region between Indel marker Y7-12 and SSR marker Y7-38, which also showed significant effects on grain width and thousand grain weight. Scanning electron microscopy analysis of the glume's epidermal cells showed that the cell length and width of NIL-qGL7IL188 was higher than NIL-qGL7Nip, indicating that qGL7 increases grain size and weight by regulating cell expansion. Conclusions: In this study, we detected 12 QTLs regulating grain size and weight using an introgression line from a cross between an Oryza sativa and Oryza minuta. Of these loci, we confirmed and delimited the qGL7 to a ~261 kb region. These results provide a basis for map-based cloning of the qGL7 gene and useful information for marker assisted selection in rice grain quality improvement.
Grouping of Genomic Markers in Populations With Family Structure
Authors: Wittenburg Dörte , Doschoris Michael , Klosa Jan 
Published: August 2020

Abstract
Background : Linkage and linkage disequilibrium (LD) between genome regions cause dependencies among genomic markers. Due to family stratification in populations with non-random mating in livestock or crop, the standard measures of population LD such as $r^2$ may be biased. Grouping of markers according to their interdependence needs to account for the actual population structure in order to allow proper inference in genome-based evaluations. Methods : he derivation of the covariance between markers in a population consisting of half- or full-sib families is described: it requires a genetic map and haplotype information of the common parent(s). A strategy, available in the literature, for grouping of markers based on a measure of population LD has been adapted to account for the dependence between markers if family stratification is present. Groups are built depending on the strength of association between markers; largest groups are built at first. As an option, a representative marker is selected for each group. Results : We provide an implementation of the theoretical covariance between biallelic markers for half- or full-sib families and the calculation of representative markers. In case studies, we have shown that the number of groups comprising dependent markers was smaller and representative SNPs were spread more uniformly over the investigated chromosome region when the family stratification was respected compared to a population-LD approach. In a simulation study, we observed that sensitivity and specificity of a genome-based association study improved if selection of representative markers took family structure into account. Conclusions : We offer an R~package for calculating the pairwise dependence between markers when family stratification is present. Chromosome segments which frequently recombine in the underlying population can be identified from the matrix of pairwise dependence between markers. Furthermore, grouping of genomic markers according to their interdependence is available. Representative markers can be exploited, for instance, for dimension reduction prior to a genome-based association study or the grouping structure itself can be employed in a grouped penalization approach.
Effect of the Colombian Emergency Department Triage Policy on the timeliness of ED care and the mortality in patients with cerebrovascular attack: A controlled interrupted time series analysis
Authors: Vallejo Carlos Eduardo , Patiño-Lugo Daniel Felipe , Aguirre-Acevedo Daniel Camilo , Acosta Juan Pablo 
Published: August 2020

Abstract
Background: Ischemic Cerebrovascular Accident (CVA) is the second cause of death and one of the leading causes of disability in the world. In Colombia, there is a prevalence of 0.16% and a mortality rate of 16.82 deaths per 100,000 inhabitants. This study assessed the Colombian Ministry of Health’s Emergency Department Triage Policy (TP) on the timeliness of hospital care, the mortality, and change in reperfusion therapy of patients with the first episode of an CVA in the Emergency Department (ED) of a hospital in the city of Medellín.Methods: A controlled interrupted time series analysis between January 2011 and November 2017 was performed in one emergency department using segmented regression analysis. The emergency department of other hospital was used as a control. Data were aggregated by month for both ED, including 60 pre-intervention and 23 intervention points.Results: No decrease in the timeliness of care in the ED was found in patients with a stroke after implementing the Colombian Ministry of Health’s TP (12.55 minutes, IC -17.07, 42.17; p: 0.4). The TP intervention produced a level change in the timeliness of care in Triage I and II subgroups, of 28.34 minutes (IC 95% 16.9, 39.79; p valor 0.00), there was no change in the trend. The classification of Triage I and II was more frequent in the post-intervention period. There was evidence of the increase in reperfusion therapy with tissue plasminogen activator (rTPA) in 4% (95% CI – 0.08, - 0.01; p value 0.01). Mortality increased 4% in the post-intervention period without being statistically significant (0.04, IC 95% - 0.08 – 0; p valor 0.06). There were no significant changes in the same outcomes in the control group.Conclusions: The implementation of the triage policy allowed improving the timeliness of ED care only in patients with ischemic CVA classified as Triage I and II, in an Emergency Department in the city of Medellín, Colombia.
Does Pesticide Exposure Affect Male Farmer’s Lower Urinary Tract Symptoms?
Authors: Kim Min Gi , Ji-Young Song , Roh Sangchul 
Published: August 2020

Abstract
Purpose: Research on the relationship between lower urinary tract symptoms and pesticides is limited. Thus, we investigated the relationship between full-time male farmers’ exposure to organophosphorus and pyrethroid pesticides and the degree of lower urinary tract symptoms. Methods: Four metabolites of each of organophosphate and pyrethroid in urine from 348 full-time male farmers were analysed. Subjects’ pesticide-related characteristics including international prostate symptoms score (IPSS) and pesticide poisoning symptoms within 48 hours of exposure to pesticides and general characteristics were assessed through one-to-one interviews using a structured questionnaire. Findings: Lower urinary tract symptoms of full-time male farmers with 1-2 pesticide poisoning symptoms (odds ratio [OR] 2.63, 95% confidence interval [CI]1.21-5.73) and ≥3 symptoms (OR 10.36, 95% CI 2.18-49.15) were significantly higher than those without symptoms. The odds ratios of two- and three-fractile groups of pyrethroid urinary metabolites were 2.50 (95% CI 1.23-5.06) and 2.72 (95% CI 1.37-5.43), respectively, compared to the one fractile group. Conclusion: Relationships among pesticide poisoning symptoms within 48 hours of exposure to pesticides, total amount of pyrethroid pesticide metabolites in urine, and the lower urinary tract symptoms of male farmers were observed in the present study, and this needs further investigation.
Perceived stress of clinical practice among associate degree nursing students in Taiwan
Authors: Wu Chia-Shan , Rong Jiin-Ru , Huang Mei-zen 
Published: August 2020

Abstract
Background: Clinical placements play an important role in helping nursing students to achieve clinical competence, but these placements can be highly challenging and stressful. This study aimed to examine factors associated with perceived stress of clinical practice and to investigate the effect of health status on perceived stress.Methods: This was a cross-sectional quantitative study conducted among 724 associate nursing degree students in Southern Taiwan.Results: Health status scores varied from 28–139, with an average of 68.40 (SD = 25.75). Perceived stress scores ranged from 0–95 points with an average score of 36.65 (SD ± 15.95). Factors linked to perceived stress were general health status, practice subject, practice grade, gender, and sleeping hours. The CART analysis showed health status as the most important predictor to perceived stress.Conclusions: General health status can be used as a measure to predict vulnerability to stress and to identify students who are most vulnerable.
Management of Anticoagulated Patients in Dentoalveolar Surgery: A Retrospective Study Comparing Bridging Versus Unpaused Vitamin K Antagonist Medication.
Authors: Buchbender Mayte , Rößler F , Kesting Marco , Frohwitter Gesche , Adler Werner , Rau Andrea 
Published: August 2020

Abstract
Objectives: The aim of this study was to investigate the occurrence of postoperative bleeding following dentoalveolar surgery in patients with either continued vitamin K antagonist medication or perioperative bridging using heparin. Study design: A retrospective study was performed analyzing patients who underwent tooth extraction between 2012 and 2017. Patients were retrospectively allocated into two comparative groups: un-paused vitamin K antagonist medication versus bridging using heparin. A healthy, non-anticoagulated cohort with equivalent surgery served as a control group. Main outcome measures were: the occurrence and frequency of postoperative bleeding, the number of removed teeth, the surgical technique of tooth removal (extraction/ osteotomy/ combined extraction and osteotomy) and the prothrombin time.Results: In total, 475 patients were included in the study with 170 patients in the group of un-paused vitamin K antagonist medication VG, 135 patients in the Bridging group BG and 170 patients in the control group CG. Postoperative bleeding was significant: CG vs.VG p=0.004; CG vs. BG p<0.001, BG vs.VG p<0.001. A significant correlation of number of the extracted teeth in the BG (p=0.014) and no significance in VG (p=0.298) and CG (p=0.210) and in the BG vs. VG and CG with p<0.001 in terms of surgical intervention extraction. No difference observed in terms of prothrombin time.Conclusion: Bridging increases the risk for bleeding compared to un-paused vitamin K antagonist medication. The perioperative management of anticoagulated patients requires a well- coordinated interdisciplinary teamwork to minimize or at best avoid both: postoperative bleeding and thromboembolic incidences.