bluuebunny/crossref_metadata_2025_split huggingface dataset:
LncRNA SNHG8 accelerates proliferation and inhibits apoptosis in HPV‐induced cervical cancer through recruiting EZH2 to epigenetically silence RECK expression
Authors: Qu Xiaohui , Li Yuanyuan , Wang Lin , Yuan Ningxia , Ma Meng , Chen Yao 
Published: January 2020

AbstractInfection of human papillomaviruses (HPVs), such as subtypes HPV16 and HPV18 is carcinogenic to human and is prominent cause of HPV‐positive cervical carcinoma (CC). A closer investigation into the mechanism of HPV‐induced CC may stimulate the generation of an improved therapy treating cervical cancer. Our study herein interrogated the function of a small nucleolar RNA host gene 8 (SNHG8) in HPV‐induced CC. As a result, a notable increase of SNHG8 in HPV‐induced CC cells was found compared with HPV‐negative CC cells. Functionally, it identified that SNHG8 aggravated the cell proliferation and migration in Cell Counting Kit‐8 and transwell assays. Besides, flow cytometry apoptosis assay displayed that blockade of SNHG8 exacerbated apoptosis of HPV‐positive CC cells. As detected by fluorescence in situ hybridization analysis and subcellular fractionation assay, SNHG8 was primarily expressed in the nucleus and exerted suppressive role on reversion inducing cysteine‐rich protein with kazal motifs (RECK) expression, which implied a potential transcriptional regulation of SNHG8 on RECK level. Mechanically, SNHG8 was disclosed to interact with enhancer of zeste homolog 2 (EZH2) based on RNA immunoprecipitation assay. ChIP assay further unveiled the occupancy of EZH2 in the promoter region of RECK. An additional chromatin immunoprecipitation assay highlighted that SNHG8 intensified the enrichment of EZH2 and H3K27me3 in RECK promoter region. Altogether, it reflected that SNHG8 recruited EZH2 to downregulate RECK expression, leading to HPV‐induced CC aggravation.
A robust two‐gene signature for glioblastoma survival prediction
Authors: Pan Yuhualei , Zhang Jian‐Hua , Zhao Lianhe , Guo Jin‐Cheng , Wang Song , Zhao Yushang , Tao Shaoxin , Wang Huan , Zhu Yan‐Bing 
Published: January 2020

AbstractGlioblastoma multiforme (GBM) is a highly malignant brain tumor. We explored the prognostic gene signature in 443 GBM samples by systematic bioinformatics analysis, using GSE16011 with microarray expression and corresponding clinical data from Gene Expression Omnibus as the training set. Meanwhile, patients from The Chinese Glioma Genome Atlas database (CGGA) were used as the test set and The Cancer Genome Atlas database (TCGA) as the validation set. Through Cox regression analysis, Kaplan‐Meier analysis, t‐distributed Stochastic Neighbor Embedding algorithm, clustering, and receiver operating characteristic analysis, a two‐gene signature (GRIA2 and RYR3) associated with survival was selected in the GSE16011 dataset. The GRIA2‐RYR3 signature divided patients into two risk groups with significantly different survival in the GSE16011 dataset (median: 0.72, 95% confidence interval [CI]: 0.64‐0.98, vs median: 0.98, 95% CI: 0.65‐1.61 years, logrank test P < .001), the CGGA dataset (median: 0.84, 95% CI: 0.70‐1.18, vs median: 1.21, 95% CI: 0.95‐2.94 years, logrank test P = .0017), and the TCGA dataset (median: 1.03, 95% CI: 0.86‐1.24, vs median: 1.23, 95% CI: 1.04‐1.85 years, logrank test P = .0064), validating the predictive value of the signature. And the survival predictive potency of the signature was independent from clinicopathological prognostic features in multivariable Cox analysis. We found that after transfection of U87 cells with small interfering RNA, GRIA2 and RYR3 influenced the biological behaviors of proliferation, migration, and invasion of glioblastoma cells. In conclusion, the two‐gene signature was a robust prognostic model to predict GBM survival.
Identification of microenvironment‐related genes with prognostic value in clear cell renal cell carcinoma
Authors: Zhang Zhao , Li Zeyan , Liu Zhao , Zhang Xiang , Yu Nengwang , Xu Zhonghua 
Published: January 2020

AbstractClear cell renal cell carcinoma (ccRCC) is the most common type of kidney tumor. Previous studies have shown that the interaction between tumor cells and microenvironment has an important impact on prognosis. Immune and stromal cells are two vital components of the tumor microenvironment. Our study aimed to better understand and explore the genes involved in immune/stromal cells on prognosis. We used the Estimation of STromal and Immune cells in MAlignant Tumor tissues using Expression data algorithm to calculate immune/stromal scores. According to the scores, we divided ccRCC patients from The Cancer Genome Atlas database into low and high groups and identified the genes which were differentially expressed and significantly associated with prognosis. The result of functional enrichment analysis and protein‐protein interaction networks indicated that these genes mainly were involved in extracellular matrix and regulation of cellular activities. Then another independent cohort from the International Cancer Genome Consortium database was used to validate these genes. Finally, we acquired a list of microenvironment‐related genes that can predict prognosis for ccRCC patients.
All‐trans‐retinoid acid induces the differentiation of P19 cells into neurons involved in the PI3K/Akt/GSK3β signaling pathway
Authors: Fu Fang , Li Lu‐Shan , Li Ru , Deng Qiong , Yu Qiu‐Xia , Yang Xin , Pan Min , Han Jin , Zhen Li , Zhang Li‐Na , Lei Ting‐Ying , Li Dong‐Zhi , Liao Can 
Published: January 2020

AbstractThe pluripotent mouse embryonal carcinoma cell line P19 is widely used as a model for research on all‐trans‐retinoid acid (RA)‐induced neuronal differentiation; however, the signaling pathways involved in this process remain unclear. This study aimed to reveal the molecular mechanism underlying the RA‐induced neuronal differentiation of P19 cells. Real‐time quantitative polymerase chain reaction and Western blot analysis were used to determine the expression of neuronal‐specific markers, whereas flow cytometry was used to analyze cell cycle and cell apoptosis. The expression profiles of messenger RNAs (mRNAs) in RA‐induced neuronal differentiation of P19 cells were analyzed using high‐throughput sequencing, and the functions of differentially expressed mRNAs (DEMs) were determined by bioinformatics analysis. RA induced an increase in both class III β‐tubulin (TUBB3) and neurofilament medium (NEFM) mRNA expression, indicating that RA successfully induces neuronal differentiation of P19 cells. Cell apoptosis was not affected; however, cell proliferation decreased. We found 4117 DEMs, which were enriched in the phosphoinositide 3‐kinase/protein kinase B (PI3K/Akt) signaling pathway, Wnt signaling pathway, and cell cycle. Particularly, a few DEMs could be identified in the PI3K/Akt signaling pathway networks, such as PI3K, Akt, glycogen synthase kinase‐3β (GSK3β), cyclin‐dependent kinase 4 (CDK4), P21, and Bax. RA significantly increased the protein expression of PI3K, Akt, phosphorylated Akt, GSK3β, phosphorylated GSK3β, CDK4, and P21, but it reduced Bax protein expression. The Akt inhibitor affected the increase of TUBB3 and NEFM mRNA expression in RA‐induced P19 cells. The molecular mechanism underlying the RA‐induced neuronal differentiation of P19 cells is potentially involved in the PI3K/Akt/GSK3β signaling pathway. The decreased cell proliferation ability of neuronally differentiated P19 cells could be associated with the expression of cell cycle proteins.
Autophagy decreases alveolar epithelial cell injury by regulating the release of inflammatory mediators
Authors: Fan Tao , Yang Shuo , Huang Zhixin , Wang Wei , Guo Xiaobo , Pan Shize , Zhang Boyou , Xu Yao , Fang Yifan , Mao Zhangfan , Hu Hao , Geng Qing 
Published: January 2020

AbstractTo research the impact of autophagy on alveolar epithelial cell inflammation and its possible mechanism in the early stages of hypoxia, we established a cell hypoxia–reoxygenation model and orthotopic left lung ischemia–reperfusion model. Rat alveolar epithelial cells stably expressing GFP‐LC3 were treated with an autophagy inhibitor (3‐MA) or an autophagy promoter (rapamycin), followed by hypoxia–reoxygenation treatment for 2, 4, and 6 hr in vitro. In vivo, 20 male Sprague Dawley rats were randomly divided into four groups (model group: No blocking of the hilum in the left lung; control group: Blocking of the hilum in the left lung for 1 hr with dimethyl sulfoxide lavage; 3‐MA group: Blocking of the hilum in the left lung for 1 hr with 100 ml/kg of 3‐MA (5 μmol/L) solution lavage; and rapamycin group: Blocking of the hilum in the left lung for 1 hr with 100 ml/kg of rapamycin (250 nmol/L) solution lavage) to establish an orthotopic left lung ischemia model. This study demonstrated that rapamycin significantly suppressed the nuclear factor kappa B signaling pathway and limited the expression of proinflammatory factors. A contrary result was found after the 3‐MA pretreatment. These findings indicate that autophagy reduces ischemia–reperfusion injury by repressing inflammatory signaling pathways in the early stages of hypoxia in vitro and in vivo. Autophagy could be a new protective method for application in lung ischemia–reperfusion injury.
miR‐873‐3p targets HDAC4 to stimulate matrix metalloproteinase‐13 expression upon parathyroid hormone exposure in rat osteoblasts
Authors: Malavika Desai , Shreya Srinivasan , Raj Priya Vembar , Rohini Muthukumar , He Zhiming , Partridge Nicola C. , Selvamurugan Nagarajan 
Published: January 2020

AbstractMatrix metalloproteinase‐13 (MMP‐13) plays a predominant role in endochondral bone formation and bone remodeling. Parathyroid hormone (PTH) stimulates the expression of MMP‐13 via Runx2, a bone transcription factor in rat osteoblastic cells (UMR106‐01), and histone deacetylase 4 (HDAC4) acts as a corepressor of Runx2. Moreover, microRNAs (miRNAs) play an important role in regulating genes posttranscriptionally. Here, we hypothesized that PTH upregulates the miRNAs targeting HDAC4, which could lead to increased Runx2 activity and MMP‐13 expression in rat osteoblastic cells. We identified several miRNAs that putatively target rat HDAC4 using bioinformatics tools. miR‐873‐3p was significantly upregulated by PTH in rat osteoblasts. miR‐873‐3p overexpression downregulated HDAC4 protein expression, increased Runx2 binding at the MMP‐13 promoter, and increased MMP‐13 messenger RNA expression in UMR106‐01 cells. A luciferase reporter assay identified the direct targeting of miR‐873‐3p at the 3′‐untranslated region of HDAC4. Thus, miR‐873‐3p targeted HDAC4 and relieved the corepressor effect of HDAC4 on Runx2 for MMP‐13 expression in rat osteoblasts. This study advances our knowledge of posttranscriptional gene regulation occurring in bone and bone‐related diseases and clarifies the role of miRNAs as diagnostic biomarkers.
E35 ablates acute leukemia stem and progenitor cells in vitro and in vivo
Authors: Chen Yingyu , Zheng Jing , Gan Donghui , Chen Yanxin , Zhang Na , Chen Yuwen , Lin Zhenxing , Wang Wenfeng , Chen Haijun , Lin Donghong , Hu Jianda 
Published: January 2020

AbstractLeukemia stem cells (LSCs) have critical functions in acute leukemia (AL) pathogenesis, participating in its initiation and relapse. Thus, identifying new molecules to eradicate LSCs represents a high priority for AL management. This work identified E35, a novel Emodin derivative, which strongly inhibited growth and enhanced apoptosis of AL stem cell lines, and primary stem and progenitor cells from AL cases, while sparing normal hematopoietic cells. Furthermore, functional assays in cultured cells and animals suggested that E35 preferentially ablated primitive leukemia cell populations without impairing their normal counterparts. Moreover, molecular studies showed that E35 remarkably downregulated drug‐resistant gene and dramatically inhibited the Akt/mammalian target of rapamycin signaling pathway. Notably, the in vivo anti‐LSC activity of E35 was further confirmed in murine xenotransplantation models. Collectively, these findings indicate E35 constitutes a novel therapeutic candidate for AL, potentially targeting leukemia stem and progenitor cells.
Determination of pH and acidity in green coffee using near‐infrared spectroscopy and multivariate regression
Authors: Araújo Cintia da Silva , Macedo Leandro Levate , Vimercati Wallaf Costa , Ferreira Adésio , Prezotti Luiz Carlos , Saraiva Sérgio Henriques 
Published: January 2020

AbstractBACKGROUNDCoffee is a raw material of global interest. Due to its relevance, this work evaluated the performance of calibration models constructed from spectral data obtained using near‐infrared spectroscopy (FT‐NIR) to determine the pH values and acidity in coffee beans in a practical and non‐destructive way. Partial least squares regression was used during the calibration and the cross‐validation to optimize the number of latent variables. The predictive capacity of the spectral pre‐processing methods was also accessed.RESULTSThe results obtained showed that the best methods of pre‐processing were the first derivative for the pH variable and the standard normal variate for the acidity, which produced models with correlations of 0.78 and 0.92, ratios of prediction to deviation of 2.061 and 2.966 and biases of −0.00011 and −0.152 to test set validation, respectively. The average errors between predicted and experimental values were lower than 7%.CONCLUSIONSFT‐NIR was successfully applied to predict properties related to the quality of coffee. The method was demonstrated to be a fast and non‐destructive tool which allows the rapid inline evaluation of samples facilitating industrial and commercial processing. © 2020 Society of Chemical Industry
Discrimination of Chain Branching Models in Addition Diene Polymerizations
Authors: L. T. Brandão Amanda , L. Alberton André , C. Pinto José 
Published: January 2020

AbstractThe present work analyzes whether discrimination of chain branching models is possible in addition diene polymerizations, based solely on average molecular weights and monomer conversions monitored during the reaction course, as usually performed in most quality control labs of industrial plants. In addition, it is verified whether the analyzed models present enough flexibility to fit data generated with the other rival models. Three kinetic models are considered, and kinetic parameters are varied in order to represent average molecular weights and monomer conversions that are typically found in addition diene polymerizations. The results show that model discrimination is indeed feasible with few experiments, even when only average molecular weights measured through GPC analyses are available, which can be of significant practical importance at plant site for critical characterization of product properties and process performance.
Coupled transfer function model for the evaluation of implanted cables safety in MRI
Authors: Kabil Julie , Felblinger Jacques , Vuissoz Pierre‐André , Missoffe Alexia 
Published: January 2020

PurposeMultiple medical‐device leads implanted next to each other are often encountered in clinical practice. The aim of this work is to study a coupled transfer function model to evaluate the safety of these coupled leads submitted to the RF field of a 1.5T MRI scanner for a constant distance between both leads.MethodsThe effect of coupling on the heating of 2 cables with different termination conditions is evaluated experimentally. The coupled and single transfer functions are determined experimentally and used to predict the relative temperature increases of both cables alone and coupled. Two different coupled models, an additive model and a global model, are proposed. The coupled transfer functions are also simulated.ResultsThe coupling between cables has a strong influence on the resulting heating at the electrodes. The coupled additive transfer function model is a relevant tool to evaluate the heating of coupled leads separated by a constant distance. The global model underestimates the heating in one of the coupled cases by about 30%. The measured coupled transfer functions coincide with the simulated models.ConclusionIt is necessary to take into account the coupling effect between leads to evaluate the safety of implanted devices. This work shows that, in the case of 2 cables separated by a constant distance, that an experimentally determined coupled transfer function allows estimation of the heating of the 2 electrodes for a given incident field. Further work should take into account the in vivo varying distance between the 2 cables.
Characterization of urethral fibrosis in a rabbit model: Potential roles of Wnt‐β catenin pathway and epithelial to mesenchymal transition
Authors: Tai Jesse W. , Yu Hosong , Chilukuri Abinav T. , Bhargava Raag , Deshpande Rucha , Li Wei , Ongkeko Weg M. , Bhargava Valmik , Rajasekaran Mahadevan “Raj” 
Published: January 2020

AbstractAimTo elucidate the precise cellular and molecular mechanisms that underlie urethral fibrogenesis.MethodsEndoluminal electrocautery injury (using Karl Storz 10 Fr. Pediatric urethroscope) was employed in male rabbits (n = 6) to create mucosal injury. Retrograde urethrogram (RUG) and endoluminal ultrasound techniques were used to assess severity and changes in luminal cross‐sectional area. Six control rabbits were subjected to sham injury, in which the electrocautery was inserted but not powered. Urethral tissues were harvested 30 days postinjury and subjected to RNA sequencing and quantitative polymerase chain reaction (qPCR) to determine changes in gene expression. Histological, immunostaining, and Western blot studies were used to determine changes in protein expression of known markers of fibrosis (eg, collagen, Integrinαv, GIV/Girdin, transforming growth factor‐β (TGF‐β), and pSMAD1,2,3).ResultsTrichrome staining confirmed increased connective tissue in urethral scar tissues. Immunostaining revealed a potential role for epithelial to mesenchymal cell transition (EMT) and positive labeling for all fibrotic markers (eg, collagen‐1, Integrin αv, GIV/Girdin, transforming growth factor‐β (TGF‐β), and SMAD1,2,3). Western blot analysis confirmed increased protein levels of these fibrotic markers.ConclusionOur RNA sequencing and qPCR studies, in conjunction with our protein data, suggest that urethral mucosal fibrogenesis may be mediated by novel fibrogenic signaling pathways involving Wnt‐β catenin, TGF‐β, GIV/Girdin, and EMT which lead to increased collagen deposition. Therapeutic strategies targeting these pathways may be beneficial in attenuating fibrogenesis and stricture progression.
Electrochemically triggered degradation of energetic material FOX‐7 (2,2‐dinitroethene‐1,1‐diamine) — Structural, redox, and spectrometric characterization in solution
Authors: Šimková Ludmila , Ludvík Jiří 
Published: January 2020

AbstractThis work represents systemati11c electrochemical and spectrometric characterization of 2,2‐dinitroethene‐1,1‐diamine, the energetic material (explosive) known as FOX‐7, in aqueous as well as various organic solvents. On the basis of results obtained from combination of electrochemistry with ultraviolet‐visible–near‐infrared (UV‐vis‐NIR), infrared (IR), nuclear magnetic resonance (NMR), electron paramagnetic resonance (EPR), mass spectrometry (MS), gas chromatography (GC), and high‐performance liquid chromatography (HPLC) methods, the actual structure of FOX‐7 in various media was revealed, its reducibility was described, and radical intermediates as well as most of the products were intercepted and identified. Instead of expected cathodic reduction of the dinitro derivative, electrochemically initiated spontaneous degradation accompanied by release of gaseous products in all media was found.
Monitoring real‐time curing of epoxies in situ using single‐sided NMR
Authors: Kelley Mackenzie , Abdol Nastaran , Soroushian Parviz , Keating Kristina , Balachandra Anagi M. , Meldrum Tyler 
Published: January 2020

AbstractAdhesively bonded joints using epoxy are widely used in aircraft and aerospace structures. Quality control and defect detection during epoxy curing in such applications is critical. We used single‐sided nuclear magnetic resonance (NMR) to nondestructively probe and spatially resolve the change in the characteristic NMR relaxation time (T2) of epoxies during curing on a substrate. Time‐dependent T2 values were fit to a Weibull function to model temporal changes in the NMR measurables. Our results demonstrate that the reduction in molecular mobility of various epoxy/curing agent mixtures occurs more rapidly at the interface than in the bulk. Further use of single‐sided NMR to acquire spatially resolved T2 data will provide a route for elucidatory epoxy curing studies. © 2020 Wiley Periodicals, Inc. J. Polym. Sci. 2020, 58, 616–623
Mucus plugging, air trapping, and bronchiectasis are important outcome measures in assessing progressive childhood cystic fibrosis lung disease
Authors: Robinson Terry E. , Goris Michael L. , Moss Richard B. , Tian Lu , Kan Peiyi , Yilma Mignote , McCoy Karen S. , Newman Beverley , de Jong Pim A. , Long Frederick R. , Brody Alan S. , Behrje Rhett , Yates Denise P. , Cornfield David N. 
Published: January 2020

AbstractObjectiveTo determine which outcome measures could detect early progression of disease in school‐age children with mild cystic fibrosis (CF) lung disease over a two‐year time interval utilizing chest computed tomography (CT) scores, quantitative CT air trapping (QAT), and spirometric measurements.MethodsThirty‐six school‐age children with mild CF lung disease (median [interquartile range] age 12 [3.7] years; percent predicted forced expiratory volume in 1 second (ppFEV1) 99 [12.5]) were evaluated by serial spirometer‐controlled chest CT scans and spirometry at baseline, 3‐month, 1‐ and 2‐years.ResultsNo significant changes were noted at 3‐month for any variable except for decreased ppFEV1. Mucus plugging score (MPS) and QATA1 and A2 increased at 1‐ and 2‐years. The bronchiectasis score (BS), and total score (TS) were increased at 2‐year. All variables tested with the exception of bronchial wall thickness score, parenchymal score (PS), and ppFEV1, were consistent with longitudinal worsening of lung disease. Multivariate analysis revealed baseline PS, baseline TS, and 1‐year changes in BS and air trapping score were predictive of 2‐year changes in BS.ConclusionsMPS and QATA1‐A2 were the most sensitive indicators of progressive childhood CF lung disease. The 1‐year change in the bronchiectasis score had the most positive predictive power for 2‐year change in bronchiectasis.