bluuebunny/crossref_metadata_2025_split huggingface dataset:
Lameness in fattening pigs – Mycoplasma hyosynoviae, osteochondropathy and reduced dietary phosphorus level as three influencing factors: A case report
Authors: Wegner Birte , Tenhündfeld Jörg , Vogels Johanna , Beumer Marius , Kamphues Josef , Hansmann Florian , Rieger Hanna , Beilage Elisabeth grosse , Hennig-Pauka Isabel 
Published: September 2020

Abstract
Background: Multiple diagnostic procedures, their results and interpretation in a case with severe lameness in fattening pigs are described. It is shown that selected diagnostic steps lead to identification of various risk factors for disease development in the affected herd. One focus of this case report is the prioritization of diagnostic steps to verify the impact of the different conditions, which finally led to the clinical disorder. Disease is the consequence of previously acting factors, and the involved diagnostic institute is the last stage in the timeline. Some diagnostic findings might therefore no longer be significant. Assessing a sufficient dietary phosphorus (P) supply and its impact on disease development proved most difficult. The diagnostic approach based on estimated calculation of phosphorus intake is presented in detail.Case presentation: On a farrow-to-finishing farm, lameness occurred in pigs with 30-70 kg body weight. Necropsy of three diseased pigs revealed claw lesions and alterations at the knee and elbow joints. Histologic findings were characteristic of osteochondrosis. All pigs were positively tested for Mycoplasma hyosynoviae in affected joints. P values in blood did not indicate a P deficiency, while bone ashing in one of three animals resulted in a level indicating an insufficient mineral supply. Analysis of diet composition revealed a low phosphorus content in two diets, which might have led to a marginal P supply in individuals with high average daily gains with respect to development of bone mass and connective tissue prior to presentation of affected animals. Finally, the impact of dietary factors for disease development could not be evidenced in all submitted animals in this case.Conclusions: Mycoplasma (M.) hyosynoviae was identified to be an important etiologic factor for disease. Other, non-infectious factors, such as osteochondrosis and claw lesions might have favored development of lameness. In addition, a relevant marginal P supply for pigs was found in a limited time period in a phase of intense growing, but the potential interaction with infection by M. hyosynoviae is unknown. The presented case of severe lameness in fattening pigs revealed that three different influences presumably act in pathogenesis. Focusing only on one factor and ignoring others might be misleading regarding subsequent decision-making for prevention and therapy. Finally, clinical symptoms disappeared after some changes in diet composition and anti-inflammatory treatment of individual animals.
Compositional and drug-resistance profiling of pathogens in patients with severe acute pancreatitis: a retrospective study
Authors: Fan Ning , Hu Yong , Shen Hong , Liu Shengjie , Zhao Guang , Sun Lanju , Li Chunyan , Zhao Xin , Li Yanning , Wang Jianhua , Cui Yunfeng 
Published: September 2020

Abstract
Background: Infection is one of the important causes of death in patients with severe acute pancreatitis (SAP) , but the bacterial spectrum and antibiotic resistance are constantly changing. Making good use of antibiotics and controlling multi-drug-resistant (MDR) bacterial infections are of vital importance in improving the cure rate of SAP. We conducted a retrospective study in the hope of providing references for antibiotic selection and control of drug-resistant bacteria. Methods: Retrospective analysis was performed on the data of patients hospitalized in our hospital due to acute pancreatitis (AP) in the past 5 years. General data were classified and statistically analyzed. Subsequently, the bacterial spectrum characteristics and the data related to drug-resistant bacterial infection of 569 AP patients were analyzed. Finally, unconditional logistic regression analysis was conducted to analyze the risk factors of MDR infection. Results: A total of 398 patients were enrolled in this study and the hospitalization data and associated results were analyzed. A total of 461 strains of pathogenic bacteria were detected, including 223 (48.4%) gram-negative bacterial strains, 190 (41.2%) gram-positive bacterial strains and 48 (10.4%) fungal strains. The detection rates of resistance in gram-negative and gram-positive bacterial strains were 48.0% (107/223) and 25.3% (48/190), respectively. There were significant differences between the MDR group and the non-MDR group for the factors of precautionary antibiotic use, kinds of antibiotics used, receipt of carbapenem, tracheal intubation, hemofiltration and number of hospitalization days in the intensive care unit. Unconditional logistic regression revealed 2 risk factors for MDR bacterial infection. Conclusions: Our results illustrate that gram-negative bacteria were the most common pathogens in SAP infection, and the proportion of gram-positive bacteria increased notably. The rate of antibiotic resistance was higher than previously reported. Unconditional logistic regression analysis showed that using more types of antibiotics and the number of hospitalization days in the ICU were the risk factors associated with MDR bacterial infection.
French hepatitis C care cascade: substantial impact of direct-acting antivirals, but the road to elimination is still long
Authors: Brouard Cécile , Pillonel Josiane , Boussac Marjorie , de Lédinghen Victor , Rachas Antoine , Silvain Christine , Lydié Nathalie , Chevaliez Stéphane , Pioche Corinne , Durand Julien , Lot Florence , Delarocque-Astagneau Elisabeth 
Published: September 2020

Abstract
Background: Hepatitis C virus (HCV) elimination by 2030, as targeted by the World Health Organization (WHO), requires that 90% of people with chronic infection be diagnosed and 80% treated. We estimated the cascade of care (CoC) for chronic HCV infection in mainland France in 2011 and 2016, before and after the introduction of direct-acting antivirals (DAAs).Methods: The numbers of people (1) with chronic HCV infection, (2) aware of their infection, (3) receiving care for HCV and (4) on antiviral treatment, were estimated for 2011 and 2016. Estimates for 1) and 2) were based on modelling studies for 2011 and on a virological sub-study nested in a national cross-sectional survey among the general population for 2016. Estimates for 3) and 4) were made using the National Health Data System. Results: Between 2011 and 2016, the number of people with chronic HCV infection decreased by 31%, from 192,700 (95% Credibility interval: 150,900-246,100) to 133,500 (95% Confidence interval: 56,900-312,600). The proportion of people aware of their infection rose from 57.7% to 80.6%. The number of people receiving care for HCV increased by 22.5% (representing 25.7% of those infected in 2016), while the number of people on treatment increased by 24.6% (representing 12.1% of those infected in 2016).Conclusions: This study suggests that DAAs substantially impact CoC. However, access to care and treatment for infected people remained insufficient in 2016. Updating CoC estimates will help to assess the impact of new measures implemented since 2016 as part of the goal to eliminate HCV.
Has Bipolar Disorder become a predominantly female gender related condition? Analysis of recently published large sample studies
Authors: Dell'Osso Bernardo , Cafaro Rita , Ketter Terence A. 
Published: September 2020

Abstract
Bipolar Disorders are disabling and severe psychiatric disorders, commonly perceived as equally affecting both men and women. The prevalence of BD in the general population has been growing over the last decade, however, few epidemiological studies are available regarding BD gender distribution, leaving unanswered the question whether the often reported increment of BD diagnosis could be gender specific. In fact, BD in female patients can often be misdiagnosed as MDD, leaving such women non correctly treated for longer times than their male counterparts. From this perspective, we searched literature for large sample (>1000 subjects) studies published in the last decade (2010 onward) on BD patients. We included ten large sample studies that reported the gender distribution of their samples, and we therefore analysed them. Our results show a higher preponderance of female patients in every sample and sub-sample of BDI and BDII, supporting our hypothesis of an increase in BD diagnosis in females. BD in women presents with higher rates of rapid cycling, depressive polarity and suicide attempts, characteristics of non inferior severity compared to males; prompt recognition and adequate treatment of BD is therefore crucial to reduce risks and improve quality of life of affected women. In this regard, our results could lead the way for national or international epidemiological studies with the aim of more accurately assessing gender-specific prevalence of BD.
Flow Cytometric Analysis of T Lymphocytes and Cytokines in Aqueous Humor of Patients with Varicella Zoster Virus-mediated Acute Retinal Necrosis
Authors: Kang Hao , Wei Yunbo , Liu Ming , Yu Di , Tao Yong 
Published: September 2020

Abstract
Background: The purpose of this study is to investigate the aqueous humor (AH) T lymphocyte subsets and cytokines of acute retinal necrosis (ARN) to elucidate the immunologic inflammatory features of this disorder.Methods: Three patients with ARN infected with varicella zoster virus (VZV) who underwent multiple intravitreal injections of ganciclovir were enrolled in this study. The control group consisted of four non-viral infectious patients with acute anterior uveitis (AAU). Flow cytometric analysis was performed on the lymphocyte subsets from the AH and peripheral blood (PB) samples during the active phase of intraocular inflammation. Five inflammatory cytokines were measured in each AH sample and various clinical characteristics were also assessed.Results: VZV DNA was detected by real-time polymerase chain reaction (PCR) in AH from all the ARN patients, who showed higher CD8+ T lymphocytes population in AH than the AAU patients (p=0.006). CD4/CD8 ratios of T lymphocytes and the percentage of CD8+CD25+ T lymphocytes in AH were significantly lower in ARN than in AAU (p=0.006; p=0.012). In the ARN patients, the percentages of CD4+ and CD8+ T lymphocytes in AH were higher than those found in PB. The percentage of CD4+CD25+ T lymphocytes in AH was significantly higher than the proportion in PB in the AAU patients (p=0.001). Immunoregulatory cytokine Interleukin-10 in AH was significantly elevated in the ARN patients in comparison with the case of the AAU patients (p=0.036). In ARN, the copy number of VZV DNA in AH positively correlated with the percentage of CD8+ T lymphocytes in AH and negatively correlated with the CD4/CD8 ratio in AH during the course of disease treatment (p=0.009, r=0.92; p=0.039, r=-0.834).Conclusion: The ARN patients caused by VZV had different intraocular T lymphocyte subsets and cytokines profile than those of the non-viral infectious patients. High percentages of CD8+ T lymphocytes and low CD4/CD8 T cell ratios may be a potential biomarker for diagnosis of viral-infectious uveitis. T lymphocytes examination at the inflammatory sites has the potential to become a useful research tool for differentiating viral and non-viral uveitis.
Marine Furanocembranoids-Inspired Macrocycles Enabled by Pd-catalyzed Unactivated C(sp3)-H Insertion Reaction of Donor/Donor Carbenes
Authors: Hao Jiping , Guo Xueying , He Shijun , Xu Zhongliang , Chen Lu , Li Zhongyu , Song Bichao , Zuo Jianping , Lin Zhenyang , Yang Weibo 
Published: September 2020

Abstract
Biomimetic modularization and function-oriented synthesis of structurally diversified natural product-like macrocycles in a step-economical fashion is highly desirable. Inspired by marine furanocembranoids, herein, we unprecedentedly synthesized diverse alkenes substituted furan-embedded macrolactams via a modular biomimetic assembly strategy. The success of this assembly is the development of crucial Pd-catalyzed carbene coupling between ene-yne-ketones as donor/donor carbene precursors and unactivated Csp3‒H bonds which represents a great challenge in organic synthesis. Notably, this method not only obviates the use of unstable, explosive, and toxic diazo compounds, but also can be amenable to allenyl ketones carbene precursors. DFT calculations demonstrated that a 1,4-Pd shift could be involved in the mechanism. Moreover, the collected furanocembranoids-like macrolactams showed significant anti-inflammatory activities against TNF-α, IL-6, and IL-1β and the low cytotoxicity is comparable to Dexamethasone.
Predicting Visual Acuity in Bietti Crystalline Dystrophy: Evaluation of Image Parameters
Authors: Huang Chu-Yen , Kang Eugene Yu-Chuan , Yeh Lung-Kun , Wu An-Lun , Liu Pei-Kang , Huang I-Wen , Ryu Joseph , Liu Laura , Wu Wei-Chi , Lai Chi-Chun , Chen Kuan-Jen , Wang Nan-Kai 
Published: September 2020

Abstract
Background: To analyze multiple imaging modalities in patients with Bietti crystalline dystrophy (BCD) and to investigate which factors from these modalities are associated with best corrected visual acuity (BCVA).Methods: In this prospective observational study, the eyes were separated into group 1 (BCVA £ 20/200) and group 2 (BCVA > 20/200). Data including BCVA and characteristic findings from near-infrared reflectance (NIR) imaging, fundus autofluorescence (FAF), and spectral domain-optic coherence tomography (SD-OCT) were analyzed and compared. The outcome measures of multimodal imaging were evaluated for correlation with BCVA.Results: NIR is a good diagnostic tool for detecting either crystalline or sclerotic vessels in BCD. Patients in group 1 tended to have a thinner choroid (P = 0.047) with ellipsoid zone (EZ) disruption (P = 0.011). Calculation of the area under the curve indicated that EZ disruption detected on SD-OCT could be a good predictor of legal blindness in BCD.Conclusion: For the diagnosis of BCD, NIR could be a good diagnostic tool. Of the studied imaging modalities, we found that EZ disruption at the fovea were strongly associated with legal blindness, which could be easily assessed by SD-OCT.Trial registration: Retrospectively registered.
Associations of Sarcopenia Components With Physical Activity and Nutrition in Australian Older Adults Performing Exercise Training
Authors: Akehurst Ewelina , Scott David , Rodriguez Juan Peña , Gonzalez Carol Alonso , Murphy Jasmaine Alyce , McCarthy Helen , Dorgo Sandor , Hayes Alan 
Published: September 2020

Abstract
Background: The risk of progressive declines in skeletal muscle mass and strength, termed sarcopenia, increases with age, physical inactivity and poor diet. The purpose of this study was to explore associations of sarcopenia components with self-reported physical activity and nutrition in older adults participating in resistance training at conventional or Helsinki University Research [HUR] gyms. Methods: Muscle strength (via handgrip strength and chair stands), appendicular lean mass [ALM] (via dual energy X-ray absorptiometry) and physical performance (via gait speed over a 4-metre distance, short physical performance battery, timed up and go and 400-metre walk tests) were evaluated in a cohort study of 80 community-dwelling older adults (mean±SD 76.5B6.5 years). Pearson correlations explored associations for sarcopenia components with self-reported physical activity (via Physical Activity Scale for Elderly [PASE]) and nutrition (via Australian Eating Survey), with higher scores indicative of greater physical activity levels and better nutrition, respectively. Results: No differences in PASE were observed between HUR and conventional gyms, however HUR gym participants had a significantly higher self-reported protein intake and a trend (p = 0.055) to have higher energy intake. In both gym groups, gait speed was positively associated with self-reported physical activity (r = 0.275; p = 0.039 and r = 0.423; p = 0.044 for HUR and conventional gyms, respectively). ALM was positively associated with protein (p = 0.047, r = 0.418) and energy (p = 0.038, r = 0.435) intake in the conventional gym group. Similar associations were observed for ALM/h2 in the HUR group. None of the sarcopenia components were associated with the Australian Recommended Food Score (derived from the Australian Eating Survey) in either gym group.
Does Sexual Compulsivity Play a Role in Unprotected Intercourse? A Cross-Sectional Study Among Sexually Active STI Patients in Shanghai
Authors: Ni Yang , Liu Hengan , Gong Ruijie , Shi Mei , Zhang Shuxian , Cai Yong , Wang Suping , Zhou Pingyu 
Published: September 2020

Abstract
Background: Sexual compulsivity and its relationship with unprotected intercourse have long been an intriguing topic, but its existential meaning in the management of public health or, more precisely, STIs (sexually transmitted infections) has rarely been studied to date. This study examines whether sexual compulsivity plays a role in unprotected intercourse among STI patients and its variation regarding gender and different types of sexual partner.Method: We carried out a cross-sectional study using mixed methods including a survey of 302 men and 362 women. Two clinics were purposely chosen for the study. Data were analyzed using IBM SPSS for Windows.Results: The ages of the 664 participants ranged from 18 to 76 years, with more than half (58.73%) 26–40 years old. Of these 664 participants, 449 (157 male and 237 female) reported having unprotected intercourse during the past 6 months. Almost one-quarter of the male participants scored high on the Sexual Compulsivity Scale, compared with only 4.4% of females. Although the only statistically significant difference (p<0.01) was in relation to unprotected intercourse with a casual sexual partner, the difference between male/female and regular/casual sexual partners remained evident.Conclusions: Sexual compulsivity is evidently a potential predictor of unprotected intercourse with a casual sexual partner in male STI patients, while the use of condoms is more likely to be affected by other factors. In addition to general sexual education, counseling interventions should be provided by related health institutions, and specific intervention methods targeting each gender and different behavioral types should be considered.
Assessing the Feasibility of Heart Rate Variability as an Objective Indicator of Anxiety in Older Adults with Dementia Living in Care Homes
Authors: Quinci Milena Aiello , Astell Arlene J. 
Published: September 2020

Abstract
Objective Anxiety is reportedly prevalent in older adults with dementia living in care homes and, within this population, is most often assessed through caregiver reports. Heart rate variability (HRV) is a physiological indicator of autonomic function, whereby reduced vagally-mediated HRV is associated with a variety of anxiety symptoms and disorders. This study examines the feasibility of HRV as an indicator of anxiety in older adults with dementia living in a care home. These data were collected during a larger study examining an exercise intervention.Results: HRV data, in the form of log-transformed root mean square of the successive differences (lnRMSSD), were in line with transformed data from previous research. Additionally, there was a negative relationship between HRV and self-reported anxiety. These data provide a promising direction for the use of wrist-worn devices in future HRV research with people living with dementia in care homes.
Identification of a Glycolysis-Related Gene Signature for Survival Prediction of Ovarian Cancer Patients
Authors: Zhang Dai , Yang Si , Li Yiche , Wang Meng , Yao Jia , Zheng Yi , Deng Yujiao , Li Na , Wei Bajin , Wu Ying , Zhai Zhen , Dai Zhijun , Kang Huafeng 
Published: September 2020

Abstract
Background: Ovarian cancer (OV) is deemed as the most lethal gynecological cancer in women. The aim of this study was construct an effective gene prognostic model for OV patients.Methods: The expression profiles of glycolysis-related genes (GRGs) and clinical data of patients with OV were extracted from The Cancer Genome Atlas (TCGA) database. Univariate, multivariate, and least absolute shrinkage and selection operator Cox regression analyses were conducted, and a prognostic signature based on GRGs was constructed. The predictive ability of the signature was analyzed in training and test sets.Results: Based on nine GRGs (ISG20, CITED2, PYGB, IRS2, ANGPTL4, TGFBI, LHX9, PC, and DDIT4), a gene risk signature was identified to predict the outcome of patients with OV. The signature showed a good prognostic ability for OV, particularly high-grade OV, in the TCGA dataset, with areas under the curve of 0.709, 0.762, and 0.808 for 3-, 5- and 10-year survival, respectively. Similar results were found in the test sets, and the signature was also an independent prognostic factor. Moreover, a nomogram combining the prediction model and clinical factors was constructed.Conclusion: Our study established a nine-GRG risk model and a nomogram to better perform on OV patients’ survival prediction. The risk model represents a promising and independent prognostic predictor for OV patients. Moreover, our study of GRGs could offer guidances for underlying mechanisms explorations in the future.
Correction of Over-Dosed Insulin in a Type-2-Diabetic Associates With a Better Control of Glycemia and Arterial Hypertension: A Case Report
Authors: Abobarin-Adeagbo Abimbola , Kraya Torsten , Girndt Matthias , Pliquett Rainer U. 
Published: September 2020

Abstract
Background: An association between hypoglycemia and arterial hypertension is unknown. Here, we report a case of a chronically over-dosed insulin therapy in conjunction with uncontrolled hypertension.Case Presentation: A 73-year-old, male diabetic of Caucasian ethnicity was admitted to a neurological intermediate-care unit of a university hospital for progressive weakness due to diabetic polyneuropathy. Besides obesity (body-mass index: 31 kg/m²) and type-2 diabetes, an uncontrolled arterial hypertension was present. Diabetes therapy consisted of fixed-dose insulin (160 units QD). Symptomatic or documented hypoglycemic episodes were not reported. Hemoglobin A1c was 11.2%. Prior to hospitalization, a urapidil (60 mg thrice a day), was added to his antihypertensive medication with valsartan (160 mg BID) and bisoprolol (5mg BID). During hospitalization, metformin, empagliflozin, and dulaglutide (1.5 mg per week) were added to insulin. An intensified conventional therapy with a cumulative daily dose of 46 units was introduced. 22 months after discharge, the medical therapy consisted of metformin (1 g BID), liraglutide (1.8 mg QD), and insulin glargine (26 units QD). The antihypertensive medication was reduced to bisoprolol and valsartan, urapidil was discontinued. The follow-up visit showed Hemoglobin A1c of 6.6%, and a well-controlled blood pressure. The patient’s physical activity improved with the patient being able to leave his flat alone for the first time within 6 years. Conclusions: Undocumented, asymptomatic hypoglycemic events and post-hypoglycemic hormonal stimulation were the likely cause for the poor glycemic and blood-pressure control. A step-wise reduction of insulin concurrently translated into a better glycemic and blood-pressure control.