bluuebunny/crossref_metadata_2025_split huggingface dataset:
Feline aortic thromboembolism: Presentation, diagnosis, and treatment outcomes of 15 cats
Authors: Hassan Marwa H. , Abu-Seida Ashraf M. , Torad Faisal A. T , Hassan Elham A. 
Published: November 2020

Background: Feline aortic thromboembolism (FATE) is a fatal disease where a blood clot gets lodged into the aortic trifurcation.Aim: This study describes the diagnosis and treatment outcome of FATE in 15 clinical cases.Methods: Fifteen cats with a sudden onset of hind limb paresis/paralysis, vocalization, and pain were admitted to the surgery clinic. A full case history was obtained and clinical, orthopedic, neurologic, radiographic, electrocardiographic, and echocardiographic examinations were performed for each cat. The treatment protocol included daily administration of multiple anticoagulant drugs with different mode of actions and meloxicam for 7 successive days. Prophylactic anticoagulant therapy (clopidogrel and acetylsalicylic acid) was continued for 6 months. All data were statisticallyanalyzed and the correlation between time of admission and treatment outcome was tested using Pearson’s correlation coefficient.Results: The case history and clinical, orthopedic, and neurologic examinations revealed a sudden onset of hind limbparalysis (n = 12) or paresis (n = 3) associated with vocalization and pain, absence of trauma, cold and pale paws of hind limbs (n = 13, 86.7%) or cyanosed hind paws (n = 2, 13.3%), absence of femoral pulsation, shallow and rapid open-mouth respiration (61 ± 8 breaths/minutes), hypothermia (37.9°C ± 0.6°C) and tachycardia (155 ± 12 beats/ minutes), with a muffled heart sound in four cats (26.7%). Radiography revealed no abnormalities in the hind limbs, pelvis, and spines, cardiomegaly in five cats (33.3%), mild pleural effusion and vascular pattern of the lung in six cats (40%), and Valentine’s heart shape in four cats (26.7%). Electrocardiography (ECG) revealed an R-wave< 0.9 mV, prolongation of QRS interval in five cats (33.3%), and conduction disturbance in four cats (26.7%). Echocardiography was consistent with hypertrophic cardiomyopathy (HCM) in five cats (33.3%). A statistically significant (p = 0.023) strong negative correlation (r = −0.6) was reported between time of admission and subsequent early treatment and recovery from clinical signs. The treatment was successful in nine cats (60%), while four cats (26.7) were euthanized and two cats (13.3%) were subjected to hind limb amputation, at the owners’ requests.Conclusion: Clinical signs, radiography, ECG, and echocardiography are valuable for diagnosis of FATE. The outcome of the multiple anticoagulants therapy depends mainly upon early diagnosis and treatment within the first 6 hours from the onset of clinical signs.
Keywords: Anticoagulant, Aortic thromboembolism, Cardiomyopathy, Echocardiography, Paralysis.
Application of gene therapy in the treatment of superficial digital flexor tendon injury in horses
Authors: Aimaletdinov Alexandr , Mindubaeva Gulnur , Khalikova Svetlana , Kabwe Emmanuel , Salmakova Alexandra , Alexandrova Natalia , Rutland Catrin , Rizvanov Albert , Zakirova Elena 
Published: November 2020

Background: Tendon injuries are one of the most common causes of orthopedic disorders in horses. Such injuries involve a long course of treatment and recovery. The most promising method of treating these injuries is the use of recombinant proteins and gene therapy.
Aim: In this work, we evaluated the therapeutic efficacy of plasmid DNA (pDNA) containing two species-specific coding sequences, i.e. vascular endothelial growth factor 164 (VEGF164) and fibroblast growth factor 2 (FGF2), in the treatment of severe damage to the tendon of the superficial digital flexor.
Methods: A pDNA construct was used to restore the damaged superficial digital flexor tendon in the horse.
Results: This study showed that the administration of pDNA encoding VEGF164 and FGF2 genes at the injury area increased the regenerative activities of the damaged tendon.
Conclusion: This study shows the therapeutic properties of genetic constructs (pDNA) and contributes to theadvancements in the use of these therapies.
Keywords: Fibroblast growth factor 2, Plasmid DNA, Superficial digital flexor, Vascular endothelial growth factor.
Combination of bleomycin and cisplatin as adjuvant electrochemotherapy protocol for the treatment of incompletely excised feline injection-site sarcomas: A retrospective study
Authors: Spugnini Enrico P. , Vincenzi Bruno , Carocci Francesca , Bonichi Chiara , Menicagli Francesco , Baldi Alfonso 
Published: November 2020

Background: Feline injection-site sarcomas (FISSs) are mesenchymal tumors that can occur in cats after injections of different medical agents and are easily prone to recurrence.Aim: The aims of this study were to report treatment outcomes for cats with feline injection-site sarcomas (FISSs) treated with both bleomycin and cisplatin, per adjuvant electrochemotherapy (ECT) protocol.Methods: The medical records of cats with a diagnosis of FISS that were treated with ECT using both bleomycin and cisplatin were retrospectively evaluated. A total of 27 cats were available for statistical evaluation of their response. The cats received intravenous 20 mg/m2 bleomycin, and the tumor bed and margins were infiltrated with cisplatin at the dose of 0.5 mg/cm2. Then, the trains of permeabilizing biphasic electric pulses lasting 50 + 50 μseconds each were delivered in bursts of 1,300 V/cm using caliper electrodes under sedation. A second session was performed 2 weekslater.Results: Side effects were limited to local inflammation in three cats. Three cats developed local tumor recurrence at days 180, 180, and 545 after surgery, two cats developed recurrence and metastases at 100 and 505 days after surgery, and two cats experienced distant metastases. A median time to recurrence could not be calculated as over 80% of the study population remained disease free or were censored due to death from other causes. Mean survival time was 985 days, and median cumulative survival for all cases was 1,000 days.Conclusion: When compared to historical controls, the results of this study demonstrate the superior rates of tumorfree survival and disease-free interval. This adjuvant therapy could be a useful addition to the current options for FISS in consideration of its efficacy, limited toxicity, and ease of administration.
Keywords: Bleomycin, Cat, Cisplatin, Electrochemotherapy, Sarcoma.
Splenic T-cell lymphoma in a North American River Otter (<i>Lontra canadensis</i>)
Authors: Matt Crystal L. , Mans Christoph , Doss Grayson , Pinkerton Marie , Elsmo Betsy 
Published: November 2020

Background: Splenic lymphoma is commonly reported in domestic ferrets (Mustela putorious furo), but very rarely reported in wild Mustelidae species, including otters. One report described B-cell splenic lymphoma in an Asian smallclawed otter (Aonyx cinerea) that metastasized and was the primary reasoning for humane euthanasia (Stedman and Mills, 2014).Case Description: The current report describes a case of splenic T-cell lymphoma in a captive North American river otter (Lontra canadensis). The otter died several weeks after splenectomy and no evidence of metastasis was found on gross necropsy or histopathological evaluation.Conclusion: The splenectomy performed on this individual was presumptively curative for its splenic lymphoma. Extensive myocardial fibrosis was found, and suspected to have caused severe cardiac arrhythmia leading to acute death.
Keywords: Fibrosis, Mustelid, Neoplasia, Splenectomy
Evaluation of pigment epithelium-derived factor concentration in equine amniotic membrane homogenate and its <i>in-vitro</i> vascular endothelial growth factor inhibition effect in tears of dogs with vascularized ulcerative keratitis
Authors: Villar Tatiane , Pascoli Ana L. , Chaulagain Sabal , Fadl-Alla Bahaa A. , Martins Bianca C. 
Published: November 2020

Background: Corneal neovascularization can result from many pathological processes affecting the ocular surface leading to disturbances and opacifications that reduce corneal clarity and may impact vision. In veterinary medicine, the use of topical corticosteroid is contraindicated in the presence of ulcerative keratitis, and there is sparse research regarding safe medical alternatives to inhibit corneal neovascularization in dogs to improve visual outcome.Aim: To investigate the pigment epithelium-derived factor (PEDF) concentration in equine amniotic membrane homogenate (EAMH) and its in-vitro vascular endothelial growth factor (VEGF) inhibition in tears of dogs with vascularized ulcerative keratitis.Methods: Homogenates from 10 equine amniotic membranes (AM) were analyzed by sandwich enzyme-linked immunosorbent assay (ELISA) for quantification of equine PEDF and VEGF. Forty tear samples were collected from both eyes of dogs diagnosed with vascularized ulcerative keratitis, and 50 samples from healthy dogs. Samples from affected eyes were allocated to G1 – affected undiluted tears; G2 – affected tears diluted with phosphate-buffer solution; G3 – affected tears treated with low-concentrated EAMH; and G4 – affected tears treated with high-concentrated EAMH. Tears from the unaffected contralateral eyes were composed in G5, while G6 was composed by tears from healthy dogs (control). The presence and levels of VEGF were evaluated in all groups by Western blot and ELISA.Results: The PEDF:VEGF ratio in EAMH was 110:1. An increase in VEGF levels was observed in tears from eyes with vascularized corneal ulcers (G1) as well as in contralateral tears (G5), compared to normal dogs (G6). Highconcentrated EAMH provided a greater decrease in VEGF levels in-vitro compared to low-concentrated EAMH.Conclusion: EAMHs exhibited high concentrations of PEDF in comparison to VEGF and were able to partially decrease VEGF levels in tears of dogs with vascularized ulcers, in-vitro. Our results suggest that VEGF concentration is elevated in tears of dogs with active vascularized ulcerative keratitis in both affected and contralateral eyes compared to that of healthy dogs.
Keywords: Amniotic membrane, Corneal neovascularization, Dog, PEDF, VEGF.
Isolation of enterotoxigenic <i>Staphylococcus aureus</i> harboring <i>seb</i> gene and enteropathogenic <i>Escherichia coli</i> (serogroups O18, O114, and O125) from soft and hard artisanal cheeses in Egypt
Authors: Hegab Ola Wagih , Abdel-Latif Eman F. , Moawad Ashraf Ahmed 
Published: November 2020

Background: Soft and hard artisanal cheeses are regularly consumed in Egypt. These products are usually processed from raw milk which may harbor many pathogenic and spoilage microorganisms.Aim: To evaluate the safety of some artisanal cheeses in Egypt, such as Ras, Domiati, and Mish, through chemical and microbiological examination.Methods: One hundred and fifty random samples of traditional Ras, Domiati, and Mish cheeses (50 each) were microbiologically and chemically analyzed. Counts of total bacteria, presumptive coliform, staphylococci, yeast, and mold were estimated. Furthermore, isolation of Escherichia coli and Staphylococcus aureus was performed, followed by PCR confirmation; isolates of E. coli were examined for the presence of virulence genes; on the other hand, the detection of the five classical enterotoxin genes of S. aureus was performed using multiplex PCR. Regarding chemical analysis, moisture, salt, and acidity content were measured. Correlations between chemical and microbial findings were investigated.Results: Mean counts of total bacteria, presumptive coliform, staphylococci, yeast, and mold were (2 × 108, 3 × 106 and 1 × 107 ), (3 × 105, 5 × 10 and 5 × 102), (1 × 106, 4 × 105 and 1 × 105), (3 × 105, 1 × 105 and 5 × 105), and (7 × 103, 4 × 103 and 3 × 104) for Ras, Domiati and Mish cheeses, respectively. Serological identification of suspected E. coli revealed that E. coli O125 was isolated from Ras and Domiati samples, E. coli O18 was recovered from Ras samples, while E. coli O114 was isolated from Mish samples. PCR results revealed that all detected isolates of E. coli were positive for both iss (increased serum survival) and fimH (type 1 fimbriae) genes. Concerning isolated S. aureus, all examined products were harboring S. aureus enterotoxigenic strains, with seb and sed genes being the most common. The mean values of moisture, salt, and acidity were (30.03, 56.44, and 58.70), (3.30, 6.63, and 7.56) and (0.65, 0.68, and 0.50) for Ras, Domiati, and Mish cheeses, respectively.Conclusion: Enterotoxigenic S. aureus harboring seb gene and enteropathogenic E. coli (serogroups O18, O114, and O125) were frequently isolated from soft and hard artisanal cheeses in Egypt. Therefore, strict hygienic measures should be applied during their manufacture, handing, and distribution.
Keywords: Domiati, E. coli virulence genes, Mish, Ras, S. aureus enterotoxins.
Use of saline contrast ultrasonography in the diagnosis of complete jugular vein occlusion in a horse
Authors: Corda Andrea , Columbano Nicolò , Secchi Valentina , Scanu Antonio , Parpaglia Maria Luisa Pinna , Careddu Giovanni Mario , Passino Eraldo Sanna 
Published: November 2020

Background: Thrombophlebitis and thrombosis are the most common causes of jugular vein occlusion in horses. Medical and surgical treatments aim to recanalize the occluded vessel and reduce proximal venous congestion and edema.Case Description: The present report describes a clinical case of equine jugular vein thrombosis (JVT) with complete vein occlusion diagnosed by saline contrast ultrasonography (SCU) and confirmed by contrast venography.Conclusion: Our results demonstrated that the SCU test can be easily performed and objectively interpreted using standard ultrasound equipment; it is not expensive and it does not require x-ray exposure. The SCU test is a valid tool to assess vessel patency and presence of collateral circulation in JVT. The test could therefore be used to monitor the progression of the disease and the effectiveness of therapy against JVT in horses.
Keywords: Contrast, Equine, Jugular, Thrombus, Ultrasound.
Knowledge, attitude and perception of related toxicity of pesticide exposure in humans and animals in Ibadan, Nigeria
Authors: Oridupa O.A. 
Published: November 2020

This study reported knowledge, attitude and perception of pesticide use among farmers to determine the level of farmers’ education on use and handling of pesticides. Data on pesticide types purchased were collected from pet-shops and agro-allied stores in three states in Nigeria. Questionnaires were administered to 100 farmers within Ibadan (Oyo State) to determine specific pesticides used, handling procedures adopted by farmers, protective measures employed and perception of toxic potentials of pesticides to human handlers and exposed animal. Survey reports showed majority used DDVP, 34% of farmers were uneducated, while 42%, 18% and 6% had primary, secondary and tertiary education, respectively. About 63.5% had been farming for 11-20years, 75% kept poultry while others kept sheep/goat and cattle. About 75% sought information from extension agents on pesticide use. The farmers were aware pesticides pose health hazards to animals but only 75% knew it could affect humans, 40% knew of pesticide withdrawal period with diverse opinions on route of pesticide poisoning. The study concluded that farmers and pet-owners in Southwest Nigeria use pesticides unethically and majority are unaware of toxicity and hazards of misuse and handling of pesticides, consequently exposing themselves and animals to hazardous and toxic pesticide levels. Legislations on pesticides enacted to prevent pesticide abuse should be enforced and awareness campaigns intensified to enlighten farmers and pet-owners on potential hazard pesticides pose to themselves, animals and environment. This would eventually prevent unnecessary exposure through misuse/mishandling of pesticides and create a safer ecosystem at large.
Keywords: Attitude, Knowledge, Perception, Pesticide, Toxicity
Effects of <i>Allium cepa</i> L. peels extract on gonadotropins, testosterone and sperm variables in Oba Marshal broiler cocks
Authors: Adeleye O.E. , Adeleye A.I. , Adetomiwa A.S. , Ariyo O.E. 
Published: November 2020

Allium cepa (onion), a natural seasoning agent that contains significant amounts of potent antioxidants in its scaly leaves is used in folkloric medicine to manage several diseases globally. Antioxidants have an essential effect on sperm health parameters; however, there is limited information on the effects of Allium cepa scaly leaf extract on reproductive functions in Oba Marshal breeder cocks. This study was conducted to investigate the effects of the aqueous extract of Allium cepa scaly leaf on reproductive functions in sexually matured Oba Marshal breeder cocks. Allium cepa bulbs were obtained from a market in Abeokuta, Ogun State. Dry scaly leaves were peeled, pulverised, macerated in distilled water, filtered and concentrated. Twenty, 42 weeks old Oba Marshal breeder cocks (3.48 – 3.62 kg) were divided into 4 groups (n = 5) and treated daily for 2 weeks thus: CT (control, distilled water, 0.5 mL/kg), T2 (extract 200 mg/kg/bird), T4 (extract 400 mg/kg/bird), T8 (extract 800 mg/kg/bird). Sperm characteristics were assessed microscopically. Testosterone, follicle stimulating hormone (FSH) and luteinizing hormone (LH) were analysed using ELISA. Data were analysed using ANOVA at α0.05. Treated birds had significantly (p < 0.05) higher sperm motility, morphology but non-significant changes in sperm viability and concentration compared with the controls. Also, serum FSH and LH significantly increased, while testosterone had no significant change in test groups compared to the control. Aqueous extract of Allium cepa scaly leaf improved testicular functions and morphology in the test cocks. The reproductive function enhancement of the extract may be due to its antioxidant effect.
Keywords: Allium cepa, Breeding, Broiler cocks, Spermatogenesis, Testosterone
Alterations in haematological parameters of multiple <i>in-utero</i> insonated rabbits (<i>Oryctolagus cuniculus</i>)
Authors: Idris S.Y. , Lawal M , Enam J.S. , Muhammed M.S. , Saleh A 
Published: November 2020

The fact that ultrasound is generally perceived as a safe imaging modality due to its use of non-ionizing radiation encouraged its increasing use in the diagnosis of cyesis in Veterinary practice. Ultrasound heating during obstetric scans has the potential of increasing body temperature via absorption. The study was conducted to determine the effect of multiple prenatal ultrasounds scanning on haematological parameters of rabbit fetuses. The research involves in vivo experimental model using 16 pregnant does and exposing them to ultrasound for average of 5 minutes at day 7, 12, 15, 20, 25, 27 and 29 of gestation. They were divided into two groups as insonated and control, the restrain and scanning procedures were mimicked on the group of does that were not scanned (positive control). Upon parturition, blood samples were collected from the kits via jugular venesection into ethylene diamine tetra acetic acid containing tubes for haematology. There was a significant reduction (p < 0.05) of red blood cell count and haemoglobin concentration of insonated group. Values of packed cell volume and platelet were lower but not significant (p > 0.05) in insonated as against the control while a non-significant slight increase in total white blood cell count was seen in the control. Therefore, ultrasound heating from multiple in-utero exposure can possibly cause alterations in haematological values in Rabbits.
Keywords: Fetus, Haematology, Insonation, In-utero, Rabbit, Ultrasound
Melatonin and garlic cytoprotective-ameliorative effects on dibutyl phthalate intoxication on sperm DNA and testicular biomakers of rabbits
Authors: Shinkut M , Aluwong T , Rekwot P.I. , Nwannenna A.I. , Samuel F.U. 
Published: November 2020

The study investigated the cytoprotective and ameliorative effects of melatonin and Allium sativum (garlic) on dibutyl phthalate (DBP)-induced oxidative stress, its impact on sperm DNA integrity and testicular oxidative stress biomarkers. Forty two rabbit bucks were randomly divided into 7 groups of 6 bucks each labeled as A, B, C, D, E, F and G: The treatment were as follows: A (served as negative control, received olive oil for 16 weeks); B (served as positive control, exposed to DBP for 16 weeks, no treatment); C (given melatonin for 8 weeks, thereafter DBP for 8 weeks); D (administered garlic for 8 weeks, thereafter DBP for 8 weeks); E (exposed to DBP for 8 weeks, thereafter melatonin for 8 week); F (exposed to DBP for 8 weeks, thereafter garlic for 8 weeks); and G (exposed to DBP for 8 weeks, thereafter melatonin + garlic for 8 weeks). Ejaculated semen was collected on the last day (112th) using artificialv vagina for rabbit and pooled for each group was used for sperm DNA fragmentation index (SDFI) determination, rabbits were sacrificed and the testes harvested for determination of superoxide dismutase activity, reduced glutathione and malondialdehyde concentration. Results showed a significant increase (P = 0.0018) in the mean SDFI in group B (78.20 ± 4.72), compared to other groups. A significant increase (P ≤ 0.0001) in superoxide dismutase activity, increase reduced glutathione concentration and decrease malondialdehyde concentrations in the treatment groups compared to the DBP exposed group without treatment (group B) were observed. Melatonin and garlic demonstrated cytoprotective and ameliorative effects against DBP-induced oxidative stress in rabbit bucks.
Keywords: Dibutyl phthalate, Garlic, Melatonin, Sperm DNA, Testicular biomarkers
Outbreak and management of <i>Salmonella</i> Enteritidis infection in 2-week-old Lohmann brown pullets
Authors: Oladele O.O. , Ameji N.O. , Gurumyen G.Y. , Omoniwa O.D. , Joseph E 
Published: November 2020

Avian salmonellosis has huge economic and public health impact. In this manuscript, a case of S. Enteritidis and its management within Jos Metropolis was reported. Fiftythree carcasses of 2-weeks old pullets were presented at the poultry and fish clinic of the Veterinary Teaching Hospital, University of Jos, Nigeria for investigation. There was persistent mortality despite 5 days medication with 20% Enrofloxacin (Floxinor®, Shijiazhuang Guanghua Pharmaceutical co. Ltd, Hebei, China). Cumulative mortality within 14 days was 203 birds in a flock of 4,000. Necropsy was done and harvested were subjected to microbial analysis for bacterial isolation, identification and antibiotic susceptibility test while portions of these organs were preserved in 10% formalin for histopathology. Necropsy findings were empty crops, hepatitis with petechial hemorrhages, nephritis, congested and consolidated lungs, peritonitis, congested spleens and mild enteritis. Histologically, there were vacuolation and necrosis of renal tubular epithelia cells and interstitial infiltration with heterophils. Severe disorganization of hepatic cords, infiltration with inflammatory cells and mild necrosis of hepatocytes were observed, while there was severe congestion and diffuse hemorrhages in the lungs. Cellular infiltration within the lamina propria of small intestine with stunting and blunting of the villi were observed. Organism isolated on MacConkey agar was identified as Salmonella Enteritidis. Antibiotic susceptibility test showed the organism to be most susceptible to Streptomycin, which was administered via drinking water at dosage of 40mg/kg with good recovery of the flock. It was concluded that the occurrence of Salmonella Enteritidis infection in this flock might be from the hatchery or via ingestion of contaminated feed and water. Day old chicks should be screened for Salmonella infection and strict biosecurity should be instituted on poultry farms.
Keywords: Mortality, Necrosis, Pullets, Salmonella Enteritidis, streptomycin