bluuebunny/crossref_metadata_2025_split huggingface dataset:
Predicting Hub Genes of Glioblastomas Based on a Support Vector Machine Combined with CFS Algorithms
Authors: Qiu Chun , Li Sai , Yang Shenghui , Wang Lin , Zeng Aihui , Zhang Xufeng 
Published: August 2020

Aim:
To search the genes related to the mechanisms of the occurrence of glioma and to try to
build a prediction model for glioblastomas.

Background:
The morbidity and mortality of glioblastomas are very high, which seriously endanger
human health. At present, the goals of many investigations on gliomas are mainly to understand the
cause and mechanism of these tumors at the molecular level and to explore clinical diagnosis and
treatment methods. However, there is no effective early diagnosis method for this disease, and there are
no effective prevention, diagnosis, or treatment measures.

Methods:
Firstly, the gene expression profiles derived from GEO were downloaded. Then, differentially
expressed genes (DEGs) in the disease samples and the control samples were identified. After
that, GO and KEGG enrichment analyses of DEGs were performed by DAVID. Furthermore, the correlation-
based feature subset (CFS) method was applied to the selection of key DEGs. In addition, the
classification model between the glioblastoma samples and the controls was built by a Support Vector
Machine (SVM) based on selected key genes.

Results and Discussion:
Thirty-six DEGs, including 17 upregulated and 19 downregulated genes, were
selected as the feature genes to build the classification model between the glioma samples and the control
samples by the CFS method. The accuracy of the classification model by using a 10-fold crossvalidation
test and the independent set test was 76.25% and 70.3%, respectively. In addition, PPP2R2B
and CYBB can also be found in the top 5 hub genes screened by the protein-protein interaction (PPI)
network.

Conclusions:
This study indicated that the CFS method is a useful tool to identify key genes in glioblastomas. In addition,
we also predicted that genes such as PPP2R2B and CYBB might be potential biomarkers for the diagnosis of
glioblastomas.
Feasibility Study for the Establishment of a Multi-Story Car Park, A Case Study
Authors: Ellk Dunya S. , Mohsin Alyaa H. , Hasan Sada A. 
Published: August 2020

Background:
Cars cruising to search out a parking zone represent a key component of the traffic on urban university campuses.

Objective:
The research aims to prepare a feasibility study for a multi-story car park for Engineering College /Mustansiriayah University through public-private partnership as a solution for parking problems which consumes a lot of time and effort.

Methods:
At first, theoretical information was collected about the subject of the research; a field survey was conducted to estimate parking demand and supply. A feasibility study was prepared for a proposed four-story car park.

Results:
The field survey showed that the current car park accommodates only 48% of the peak demand, so the rest of the cars are parked in an irregular manner. The proposed multi-story car park was estimated to have a maximum capacity of 560 cars and an investment cost of (4,140,875$).

Conclusion:
The economic indicators of the project showed that it would achieve a Simple Rate of Return 5.49% of the investment cost. It has a positive net present value at 5% rate of discount, which means it is feasible, and has an internal rate of return 8.1%, also the payback period is 10 years, which means that the investor will have his investment back after 10 years of operating the project. This research contributes to the body of information by including community participation in the procurement of infrastructure.
Human Brucellosis as an Epidemic Zoonosis in Zenica-Doboj Canton (Bosnia and Herzegovina) During 2008-2018
Authors: Uzunović Selma , Skomorac Muhamed , Bašić Fatima , Kamberović Farah , Ibrahimagić Amir , Dizdarević Jasmin 
Published: August 2020

Background:
Brucellosis is associated with people living in close proximity to their animals, where conditions for disease onset and spread exist. An epidemic of brucellosis in Bosnia and Herzegovina (B&H) has persisted since 2004. Zenica-Doboj Canton is one of the most affected areas.

Objective:
To investigate the epidemiological characteristics of human brucellosis from the year 2008 to2018.

Methods:
Data collected from paper-based patients/cases reported to the Epidemiology Department were analyzed.

Results:
After 2008, the annual number of patients diagnosed with brucellosis was decreasing, except in 2017 and 2018 with 20 and 35 cases, respectively. Within the 2008-2018 period, a total of 263 human brucellosis cases were recorded, decreasing from 102 (incidence of 44.7/100,000) cases in 2008 to three cases in 2012, but increased to 35 cases in 2018. Males were predominant, with a total of 205 (77.9%) cases. The mean age of the affected patients was 39.2 years; but the most affected age group was the 25-49 years age group with 117 (44.5%) cases. Most cases (151 cases, 66%) were reported during the period of March-July, and 242 (92%) cases were from the rural areas.

Conclusion:
With the implementation of the small ruminant vaccination program in 2009, the number of infected humans had declined, while brucellosis still remains.
Role of Protein Tyrosine Phosphatase (PTPN22) Gene [C1858T] Functional Variant in Genetic Susceptibility of Psoriatic Arthritis in Kuwaiti Arabs
Authors: Al-Awadhi Adel M. , Haider Mohammad Z. , Sukumaran Jalaja , Mohammed Asiya T. , Hasan Eman AH , Bartella Youssef A. 
Published: August 2020

Background:
Psoriatic arthritis (PsA) is a chronic, systemic inflammatory arthritic disease characterized by joint inflammation that is associated with cutaneous psoriasis, and can lead to pain, swelling, or stiffness in one or more joints. It results from a complex interplay between genetic, immunologic and environmental factors. A functional variant [C1858T] in the protein tyrosine phosphatase (PTPN22) gene, which encoded Arg620Trp in the lymphoid protein tyrosine phosphatase (LYP) has been shown to be a negative regulator of T-cell activation.

Objective:
The objective of this study was to investigate an association between PTPN22 gene [C1858T] functional variant and PsA in Kuwaiti patients.

Methods:
We have investigated the association of PTPN22 gene functional variant in 102 Kuwaiti patients with psoriatic arthritis and compared it to that in 214 healthy controls. The genotypes for the PTPN22 gene [C1858T] variant were determined by using a PCR-RFLP method and confirmed by DNA sequence analysis.

Results:
The frequency of homozygous variant genotype (TT) was found to be significantly higher in PsA patients compared to that in the controls (p <0.0001). Collectively, the variant genotype was detected in homozygous and heterozygous combinations in 30% patients (p <0.0001) compared to 16% in the controls. The frequency of variant genotype was found to be highest in the early-onset PsA patients (age >25-34y). No correlation was detected between the variant genotype (TT) and gender in the Kuwaiti PsA patients.

Conclusion:
Our data show a significant association of PTPN22 gene functional variant [C1958T] with PsA in Kuwaiti patients and highlight its role in determining the genetic susceptibility along with other factors.
Morphological Characterization and Genetic Diversity of Sesame (Sesamum indicum L.) Varieties Cultivated in Ethiopia
Authors: Mawcha Kahsay T. , Gebru Mulubrhan M. , Assefa Mewael K. , Mesfin Mebrahtom , Gebre Girma G. 
Published: August 2020

Introduction:
The experiments were conducted to identify and group sesame genotypes by morphological and genetic characteristics.

Materials and Methods:
Ten nationally released sesame genotypes were obtained from Humera and Melka-Worer research centers and planted at Humera experimental sites. The number of primary branches per plant was found higher (5.93) for M-80. However, the number of nodes per plant was grouped as ‘more’ (24.13) for M-80 compared to Humera-1, which was grouped as the ‘highest’ (26.13). Plant height was tall in all the sesame genotypes studied. Likewise, the number of nodes per plant in all the genotypes was grouped as more nodes per plant i.e. greater than 15. The leaf length ranged from 7.769 cm to 6.67 cm and genotypes, Argene and K-74 had the lowest and the highest values, respectively. The variation was least in qualitative characteristics like flower colour, number of locules per pod, pod pubescence, and type of pod beak. Thousand seed weight and seed yield per kg per ha had a significance level at P=0.05. Pod length was found to be varied among the genotypes and grouped as ‘long “with a mean pod length of 2.44 cm. Hence, genotypes having a long pod length might be preferred for seed production.
The genetic characters of the genotypes were characterized and the level of polymorphism for the selected primers ranged from 56.25% to 100%. ISSR-880 was found with the least level of polymorphism while the other three (ISSR-811, ISSR-812 and ISSR-860,) were found with a high level of polymorphism. Five sesame genotypes (ADI, Humera1, Serkamo, M-80 and E) were clustered and grouped together, while three genotypes, namely, Argena, K-74, and S-Breeder appeared to have their own cluster separately.

Results:
Our study indicates that agro-morphological and genetic characterization of genotypes is useful and effective in determining polymorphisms in the sesame genotypes molecular genotyping, reproducible and it can also produce enough polymorphism to cluster and identify the genotypes.

Conclusion:
The findings presented in this paper may prove useful in the future to include more ISSR markers and induvial genotype investigation for detailed characterization of sesame germplasm that would be important for future breeding and sesame improvement program.
Group Play Therapy for Preadolescents: Post-Traumatic Stress Disorder of Natural Disaster Victims in Indonesia
Authors: Rusmana Nandang , Hafina Anne , Suryana Dodi 
Published: August 2020

Background:
This study was motivated by the failure of coping and adaptation to traumatic experiences that cause a wide, deep, and long-term snowball effect that may not be reversible.

Objective:
This study aims to test the effectiveness of the implementation of group play therapy in dealing with post-traumatic stress disorder (PTSD) victims of natural disasters in three provinces in Indonesia.

Materials and Methods:
The study employed an explanatory mixed methods design. The procedures and steps taken in this study refer to the research and development cycle. The subjects and objects of the study were 106 male and 92 female preadolescent students of junior high school in three provinces in Indonesia.

Results:
Traumatic counselling through group play therapy techniques has the effect of reducing post-traumatic stress disorder on victims of the tsunami disaster in three provinces in Indonesia. The first study conducted in Lombok West Nusa Tenggara province obtained the results of the calculation of the value μ2 = 0.021 for the value of μ1> 0.05. The second study in Palu, Central Sulawesi Province obtained the results of the calculation of the value of μ2 = 0.018 for the value of μ1> 0.05, and the third study in Anyer, Banten province obtained the results of the calculation of the value of µ2 = 0.011 for the value of µ1 > 0.05.

Conclusion:
Teaching and practising the skills of traumatic counselling using using Group Play Therapy can handle PTSD victims of natural disasters that significantly change victims.
Traditional and Advanced Neuroimaging Contributions to the Diagnosis and Differential Diagnosis of Central Nervous System Lymphoma Patients Visiting a Comprehensive Medical Center
Authors: El Dirani Ali , Hachem Zahraa , Mohanna Assaad , Zaylaa Amira J. 
Published: August 2020

Introduction:
The diagnosis of Central Nervous System Lymphoma, especially the Primary Central Nervous System Lymphoma is carried out based on brain imaging, thus avoiding an unnecessary extend of surgery. But the traditional imaging techniques, such as Computed Tomography and Magnetic Resonance Imaging, were not satisfactory.

Aims:
This study was conducted to characterize the spectrum of advanced Neuroimaging, such as the advanced Magnetic Resonance Imaging features in the Central Nervous System Lymphoma patients in a comprehensive medical center in Lebanon, and compare them to what has been described in the literature review.

Methods:
It is a retrospective exploratory study of the clinical data and imaging features for patients admitted to the emergency and radiology departments with ages above 10 years, and who were diagnosed histopathologically with intracranial lymphoma. This study may be the first to make a Radiological evaluation of Central Nervous System Lymphoma on the local population of patients over 9 years .

Results:
Results showed that the study of the Computed Tomography and Magnetic Resonance Imaging data of 10 immunocompetent patients with Central Nervous System Lymphoma concurs with the previously described patient populations, except for the gender parameter. Tumors were mostly presented in the fifth or Sixth decade and they could be solitary or multi-focal. Lesions were typically located Preprint submitted to The Open Neuroimaging Journal May 14, 2020 in the supratentorial compartment. On the brain Computed Tomography, the lesions were hyperdense, and in pre-contrast Magnetic Resonance images, the lesions appeared hypointense on T1 and hyperintense on T2-Weighted images, but hypointense with respect to the grey matter. The lesions were also surrounded with a mild to moderate edema as compared to other intracranial neoplasms, such as glioblastomas. Evaluation results showed that on post-contrast Magnetic Resonance images, the majority of lesions exhibited a homogeneous enhancement of 50%. Majority of the lesions also showed a less common heterogeneous ring-like enhancement of 40%, and revealed the uncommon absence of enhancement of 10%. Calcifications, hemorrhage, and necrosis were rare findings and were present in only one patient.

Conclusion:
As a future prospect, studying whether the advanced imaging techniques may provide not only non-invasive and morphological characteristics but also non-invasive biological characteristics and thus accurate diagnosis could be considered.
Corrosion Effect of Rice Husk Ash in Concrete Pore Solution: Response Surface Analysis
Authors: Ayobami Busari , Williams Kupolati , Tolulope Loto , Emmanuel Sadiku , Snyman Jacques , Julius Ndambuki 
Published: August 2020

Background:
Corrosion of reinforcement impedes the structural integrity of concrete infrastructures by reducing the flexural, shear and axial strength of concrete, thereby making it structurally weak.

Aim:
This research assessed the corrosion effect of rice husk as a concrete constituent for the design of long-lasting concrete infrastructures.

Materials and Methods:
To achieve the aim of this research, rice husk was air-dried for two days and then burnt at a temperature of 600oC. It was used as a partial replacement for cement at 0%, 10%, 20% and 30% replacement of cement. The concrete pore solution was extracted by mechanical means. This was used as the medium to assess the weight loss and calculate the corrosion rate at seventy-two (72) hours interval with a focus on the temperature of the environment. The corrosion inhibition of the steel rebar was determined using the weight loss method.

Results:
The result was analysed and modelled using a response surface analysis. The optimisation of the corrosion effect was also assessed using the same method. The result of the study revealed that the inhibition efficiency based on the average corrosion rate was -69.54%, which indicates that 5% of rice husk ash does not inhibit corrosion, likewise the other replacements. The corrosion inhibition of 15% replacement with rice husk ash is slightly higher than the control sample. The research revealed that the most favourable replacement in terms of corrosion rate in comparison to the other percentages is 15%.

Conclusion:
The mathematical model showed that RHA has a positive effect on the corrosion rate of mild steel. This indicates that the higher the RHA, the lower the corrosion rate. The outcome of this research will serve as a guide for concrete users, engineers, corrosion experts and researchers on the use of rice husk ash in concrete production.
Role of Vitamin D Receptor in Prediabetes
Authors: Kharb Simmi , Goel Kanika , Rajput Rajesh 
Published: August 2020

Background:
Recent epidemiological evidence points towards the potential association of vitamin D insufficiency with adverse metabolic risk and in the pathogenesis of cancer, cardiovascular diseases, type 2 diabetes and other diseases. Vitamin D exerts its action in a variety of cell types through vitamin D receptors. No reports are available in the literature regarding vitamin D and vitamin D receptor status in prediabetics. The present study was planned to compare serum 25-hydroxy vitamin D [25(OH)D] and vitamin D receptor (VDR) protein levels in prediabetic cases and normoglycemic controls.

Methods:
The present study was conducted in 80 persons who were divided into two groups, Study group (n= 40) comprised of diagnosed cases of prediabetes and control group (n=40) comprised of healthy normoglycemic controls. Serum 25-hydroxy vitamin D [25(OH)D] was analyzed by radioimmunoassay (RIA). Serum vitamin D receptor (VDR) protein was analyzed by sandwich enzyme immunoassay (ELISA).

Results:
Serum 25(OH) vitamin D levels were significantly decreased in prediabetic cases as compared to normoglycemic controls [p<0.001]. Serum Vitamin D receptor protein levels were highly significantly decreased in prediabetic cases as compared to normoglycemic controls [p<0.00]. Serum 25(OH)D levels showed a highly significant positive correlation with serum VDR levels in both the groups [p<0.001 at both levels].

Conclusion:
The findings of the present study indicate that vitamin D and VDR can serve as a possible screening marker and target for modulation of the management and alleviating the progress and complications of diabetes.
Antibiofilm Activity of ZnO/Zeolite Nanocomposite (ZnO/ZeoNC) Against Klebsiella pneumoniae and its Biocompatibility in an Animal Model
Authors: Partoazar Alireza , Bideskan Fatemeh Rahmani , Takzaree Nasrin , Dallal Mohammad Mehdi Soltan 
Published: August 2020

Background:
Infectious diseases, whether intracellular or extracellular infections, biofilm-
mediated, or medical device-associated, have always been a global public health problem,
causing millions of deaths each year. The aim of this study was to evaluate the antibiofilm activity
of ZnO/ZeoNC against K. pneumoniae along with the biocompatibility of the nanocomposite in vivo
model.

Objective:
The formation of biofilm by K. pneumoniae in the catheter-associated urinary tract
causes a nosocomial infection. In this regard, antimicrobial nanomaterials have emerged as potent
effective agents against biofilm formation. Nevertheless, nanoparticles have already been a challenge
with possible side effects such as inflammation. The ZnO/ZeoNC may exhibit anti-biofilm
property with minimal adverse effects.

Methods:
The biofilm formation of K. pneumoniae strains was exposed to ZnO/ZeoNC and then
SEM imaging was performed for morphological investigation of bacteria in biofilm state. The response
to ZnO/ZeoNC embedded polyethylene tube of the tissue of mice was also analyzed during
the 30-day experiment.

Results:
The results of this study showed that ZnO/ZeoNC has significant antibiofilm activity
against K. pneumoniae strains in its sublethal doses. The ZnO/ZeoNC also caused deformation in
K. pneumoniae biofilm. In addition, ZnO/ZeoNC also reduced inflammatory response in cell tissue
of rats subjected to polyethylene tube.

Conclusion:
ZnO/ZeoNC can be used potentially against the infections caused by K. pneumonia
biofilm without any irritability on the biotic surface such as the urinary tract.
Herstellung endlosfaserverstärkter, thermoplastischer Halbzeuge für Karosseriestrukturbauteile in Großserie
Authors: Hofbauer Daniel 
Published: August 2020

Derzeit werden in der Automobilindustrie Faser-Kunststoff-Verbunde mit thermoplastischer Matrix nur für semistrukturelle Bauteile mit geringeren Steifigkeits- und Festigkeitsanforderungen als Strukturbauteile und ohne Einbindung in die Hauptlastpfade einer Karosseriestruktur eingesetzt. Auf Basis von hohen mechanischen Anforderungen an ein Schwellerprofil einer Karosserie, soll demgegenüber eine zweischalige Bauweise unter Verwendung von mit Polyamid-Matrix vorimprägnierten, unidirektionalen Kohlenstofffasertapes zur Anwendung kommen. Infolge der extremen Belastungen ergibt sich ein Laminataufbau mit einer hohen Vorzugsrichtung in 0°-Faserorientierung und einer Dicke von bis zu 4,9 mm. Zur Herstellung derart hoher Wandstärken müssen die einzelnen unidirektionalen Tapes schichtweise aufgebaut und zu einem Laminat konsolidiert werden. Die Konsolidierung der Einzellagen kann dabei entweder direkt bei deren Ablage, auf statischen Heizpressen oder mittels Doppelbandpressen erfolgen. Für eine großserielle Herstellung ist aufgrund des kontinuierlichen Fertigungsprinzips eine Doppelbandpresse grundsätzlich von Vorteil. Anhand von experimentellen Fertigungsversuchen zur Konsolidierung der Layups konnte jedoch weder mit einer isobaren Doppelbandpresse, noch mit einer statischen Laborplattenpresse eine ausreichend gute Laminatqualität erzeugt werden. Lediglich die Veränderung des Lagenaufbaus, verbunden mit einer Reduzierung der mechanischen Eigenschaften des Laminats, erzeugte auf der Laborplattenpresse eine entsprechende Qualität. Allerdings ist die dafür erforderliche Zykluszeit für eine Großserienfertigung inakzeptabel. Somit stellt weder der untersuchte Doppelbandprozess, noch der statische Pressprozess, hinsichtlich der erzielbaren Laminatqualität und der erforderlich Anlagenkapazität für das beschriebene Schwellerprofil eine großserientaugliche Fertigungstechnologie dar. Darauf aufbauend müssen weiterführende Fertigungsstudien zum Doppelbandpressprozess mit Anpassung der Prozessparameter durchgeführt werden.