bluuebunny/crossref_metadata_2025_split huggingface dataset:
A New Approach to the Analysis of Nicarbazin and Ionophores in Eggs by HPLC/MS/MS
Authors: Dmitrovic Jasna , Durden David A 
Published: July 2020

Abstract
An HPLC/MS/MS method has been developed and validated for the quantification and confirmation of nicarbazin and ionophores (lasalocid, monensin, salinomycin, and narasin) in eggs. Nicarbazin is determined in the negative electrospray mode with a basic mobile phase that supports creation of negative ions. Consequently, our ability to maintain instrument sensitivity over time has significantly improved. The analysis of the ionophores is done in the positive electrospray mode using ammonium buffer for HPLC separation. Monitoring ammonium adduct parent ions resulted in enhanced sensitivity and better reproducibility of the ionophore analysis. The validation of this improved HPLC/MS/MS method for the detection of nicarbazin and the ionophores demonstrated excellent precision of below 10 RSD and lower LOD values (g/kg) for nicarbazin (0.018), lasalocid (0.015), monensin (0.015), salinomycin (0.033), and narasin (0.039).
Determination and Confirmation of Tulathromycin Residues in Bovine Liver and Porcine Kidney via Their Common Hydrolytic Fragment Using High-Performance Liquid Chromatography/Tandem Mass Spectrometry
Authors: Boner Pamela L , Gottschall David W , Kim-Kang Heasook 
Published: July 2020

Abstract
An accurate, reliable, and reproducible analytical method using HPLC/MS/MS for the determination of tulathromycin residues in bovine liver and porcine kidney via their common hydrolytic fragment (CP-60,300) was developed and validated. Briefly, the method involved an initial acid treatment of intact tissues, which yielded the common fragment (CP-60,300). A portion of the acid hydrolyzate was purified by SPE using a strong cation exchange cartridge. Evaporation of the purified extract was followed by reconstitution in aqueous buffer and analysis by HPLC/MS/MS under isocratic conditions. The developed method provided acceptable sensitivity for determinative surveillance of tulathromycin in porcine kidney and bovine liver with an LOQ of 7.50 and 2.75 g/g, respectively. The overall recovery and precision (CV) of 45 determinations of each tissue were 97.8 (5.3) for porcine kidney and 96.9 (7.9) for bovine liver. Accuracy, precision, linearity, specificity, and ruggedness were demonstrated. An HPLC/MS/MS method was also developed for use in these tissues as a confirmatory assay following modifications to the MS detection parameters. The confirmatory method demonstrated acceptable sensitivity for confirmatory evaluation of tulathromycin in porcine kidney and bovine liver at tolerances of 15 and 5.5 g/g, respectively.
Determination of Methanol in Cosmetics by Headspace and Multidimensional Gas Chromatography with Mass Spectrometric Detection
Authors: Yi Xionghai , Guo Dehua , Deng Xiaojun , Li Bo , Fan Xiang , Zhu Jian 
Published: July 2020

Abstract
A novel, reliable, and robust analytical method using headspace and multidimensional GC coupled to MS was successfully developed for determining the methanol content in cosmetics. The methanol was quantitatively analyzed and confirmed by heart-cutting multidimensional GC and mass selective detection in full scan mode. The average recovery was 99.8 with an RSD of 4.3; the LOQ was 5 mg/kg. The proposed method showed good accuracy and precision while minimizing erroneous results due to false positives compared with conventional single-column GC analysis.
Analytical Methods for Detection of Gluten in Food—Method Developments in Support of Food Labeling Legislation
Authors: Haraszi Reka , Chassaigne Hubert , Maquet Alain , Ulberth Franz 
Published: July 2020

Abstract
The current essential therapy of celiac disease is a strict adherence to a gluten-free diet. Besides food products that are naturally gluten-free, “very low gluten” and “glutenfree” bakery products have become available. The availability of immunochemical and other analytical methods to determine gluten markers in foods is of utmost importance to ensure the well being of gluten-sensitive individuals. The aim of this review was to evaluate if currently available methodologies are suitable to meet the requirements of food labeling standards for individual gluten source declaration, in order to achieve policy objectives. Codex Alimentarius and European Union (EU) legislation and gluten detection methodologies applicable at present have been summarized and compared. In 2009, the European Commission issued Regulation No. 41/2009 concerning the composition and labeling of foodstuffs suitable for people intolerant to gluten. This review constitutes a basis to investigate the possibility to develop a proteomic-based method for the specifc detection of gluten-containing cereals in food products, especially at or around the limits specifed in EU legislation.
Box-Behnken Design-Desirability Function Approach in Optimization of HPLC Method for Simultaneous Determination of Ibuprofen Along with Additives in Syrup Formulation
Authors: Dinç-Zor Şule , Aksu Dönmez Özlem 
Published: July 2020

Abstract

Background
The advantages of simultaneous analyses are a decrease in analysis time and a more economical use of solvents and reagents. It is desirable that HPLC analyses possess both short term and good resolution.

Objective
The aim of the current study is to develop an HPLC method for the simultaneous determination of ibuprofen, sodium benzoate, methyl paraben and propyl paraben as preservatives, and sunset yellow as a colorant, in syrup formulation.

Method
To optimize chromatographic separation conditions, multi-response optimization using the Derringer’s desirability function was employed for the development of a rapid and efficient HPLC method. The ranges of independent variables used for the optimization process were 50–60% (v/v) for acetonitrile, 5.0–7.0 for pH, and 1.0–2.0 mL/min for flow rate of the mobile phase. The effects of these variables on the output responses, such as critical resolution between sunset yellow and sodium benzoate and retention time of the last peak indicating analysis time of the method, were evaluated by statistical experimental design.

Results
Optimum conditions fixed for the simultaneous analyses were acetonitrile:phosphate buffer (60:40, v/v), pH 5.0, and a flow rate of 1.8 mL/min. The eluate was monitored using a photo diode detector set at 220 nm. Total chromatographic analysis time was approximately 3 min.

Conclusions
The developed method validated as per International Conference on Harmonization guidelines was successfully applied for the determination of five compounds in their pharmaceutical formulation.

Highlights
This efficient method has isocratic elution system and can be used for routine analyses of these compounds in similar pharmaceutical products.
Linker DNA and histone contributions in nucleosome binding by p53
Authors: Nishimura Masahiro , Arimura Yasuhiro , Nozawa Kayo , Kurumizaka Hitoshi 
Published: July 2020

Abstract
The tumour suppressor protein p53 regulates various genes involved in cell-cycle arrest, apoptosis and DNA repair in response to cellular stress, and apparently functions as a pioneer transcription factor. The pioneer transcription factors can bind nucleosomal DNA, where many transcription factors are largely restricted. However, the mechanisms by which p53 recognizes the nucleosomal DNA are poorly understood. In the present study, we found that p53 requires linker DNAs for the efficient formation of p53-nucleosome complexes. p53 forms an additional specific complex with the nucleosome, when the p53 binding sequence is located around the entry/exit region of the nucleosomal DNA. We also showed that p53 directly binds to the histone H3-H4 complex via its N-terminal 1–93 amino acid region. These results shed light on the mechanism of nucleosome recognition by p53.
Harnessing the Power of Low-tech Collaborative Learning
Authors: Sheth Monica , Samreen Naziya , Rapoport Irina , Slanetz Priscilla J , Fornari Alice , Lewis Petra 
Published: July 2020

AbstractMedical education in the United States has undergone a paradigm shift from passive learning to more interactive student-centered teaching methods. Several digital tools and platforms have been developed to assist educators in creating a high-tech, interactive classroom. However, there are many low-tech ways to engage learners and improve retention by combining collaborative learning techniques and summary exercises. Collaborative learning is an educational approach that involves the coordinated engagement of two or more learners for the purpose of completing a task or solving a problem. Such methods use a student-centered active learning model to promote higher-order cognitive tasks through active engagement in course content. Additionally, summary exercises at the end of a learning activity promote reflection and retention of learned concepts while clarifying content that may have been confusing for the learner. The purpose of this article is to describe the methodology and tips for the implementation of low-tech collaborative learning methods and summary activities into trainee educational activities to create an engaging student-centered learning environment.
Genetic diversity and phenotypic variation of the red swamp crayfish, Procambarus clarkii (Girard, 1852) (Decapoda: Astacidea: Astacidae), in China
Authors: Yi Shaokui , Zhang Long , Li Yanhe , Shi Linlin , Chen Jing , Wang Weimin , She Lei , He Jixiang 
Published: July 2020

Abstract
The red swamp crayfish, Procambarus clarkii (Girard, 1852), is a paradox in China because it has developed as a popular aquaculture species while becoming a notorious invasive species. We investigated the population genetic diversity and population dynamics of wild Chinese populations of P. clarkii based on proPO fragments and the morphological traits across these populations. The results showed that the populations of P. clarkii exhibit high genetic diversity and significant population structure, inferring that the crayfish was artificially transferred rapidly among different locations for different reasons. Results also revealed distinct phenotypic differentiation, with individuals of some populations having a shorter pleon and wider carapace width, whereas other populations have a small length to width ratio. This study provided comprehensive insights into the population dynamics and morphological variations of P. clarkii, which contribute to understanding the rapid evolution of phenotypic traits after its successful invasion. Results should help establish efficient management strategies for the control of the invasion and at the same time using the genetic information in crayfish aquaculture.
Reevaluating the Economic Injury Level for Brown Stink Bug (Hemiptera: Pentatomidae) at Various Growth Stages of Maize
Authors: Bryant Tim B , Dorman Seth J , Reisig Dominic D , Dillard DeShae , Schürch Roger , Taylor Sally V 
Published: July 2020

Abstract
Economic yield loss and reduction in grain quality from brown stink bug, Euschistus servus (Say), feeding injury in early and late stages of maize, Zea mays (Poales: Poaceae, Linnaeus), development was assessed in Virginia and North Carolina in 2018 and 2019. Varying levels of stink bug infestations were introduced to seedling maize (V2—early stage), and a range of late-stages of maize, including 1) the last stage of vegetative development (V12/V14), 2) prior to tasseling, 3) at tasseling (VT), and 4) across all tested late growth stages. Euschistus servus infestation levels included 33, 67, and 100% of maize seedlings, and 25, 50, 100, and 200% of plants during later stages. Infestations were maintained on seedling maize for 7 d, and 8 or 16 d in reproductive stages. Infestation level in seedling maize had an impact on grain yield. Infestation level and growth stage both had an impact on grain yield in reproductive maize. The percentage of discolored kernels was also affected by infestation level, but not growth stage. Regression analysis between grain yield and infestation level indicated that the average economic injury level is 7% in seedling maize (7 bugs/100 plants) and 12% (12 bugs/100 plants) from the last vegetative stages (V12/V14) through pollination (VT). The economic injury level in the late vegetative stages is only applicable when infestations are present for an extended period of time (16 d), emphasizing the need for continued scouting of maize throughout the season to make informed management decisions.
An immersed boundary method with iterative symmetric interpolation for irregular surface topography in seismic wavefield modelling
Authors: Li Xiang , Yao Gang , Niu Fenglin , Wu Di 
Published: July 2020

AbstractThe irregular free surface topography has a significant impact on simulations of seismic wave propagation. Therefore, an accurate representation of the irregular free surface is required for an accurate wavefield simulation. We propose an immersed boundary method used in fluid dynamics calculation to simulate acoustic waves with finite-difference in media with irregular surfaces. First, we set the number of ghost layers to half the length of the finite-difference stencil. Then, we define mirror points by orthogonally projecting the ghost points to fractional points below the free surface. We calculate the wavefield at these mirror points using an iterative symmetric interpolation method. Finally, we set the wavefield at the ghost points to the negative value of the wavefield of their corresponding mirror points. The proposed iterative symmetric interpolation method allows computing the wavefield at the mirror points more accurately and stably than the conventional immersed boundary methods. Numerical examples validate the accuracy and stability of this method in seismic forward modelling with strongly varying topography.
Research on nanoprocess of non-equilibrium materials byin situultra-high voltage electron microscopy
Authors: Yasuda Hidehiro , Sato Kazuhisa , Mori Hirotaro 
Published: July 2020

AbstractUltra-high voltage electron microscopy is useful for research utilizing high-penetration thickness of electron beam, in situ observation, or irradiation effects by the particle characteristics of electrons. In this review, the importance of non-equilibrium materials science research by a combination with irradiation effects and in situ observation is shown, and examples of some research are introduced. For example, crystal-amorphous-crystalline phase transition in intermetallic compounds, non-equilibrium phase transition in pure metallic nanoparticles and nucleation and growth process of electron irradiation-induced crystallization in amorphous nanoparticles will be discussed. Finally, we want to suggest the importance of exploring non-equilibrium materials science based on dynamic structures which has been unexplored.
Past Debates, Fresh Impact on Nano-Enabled Food: A Multigroup Comparison of Presumed Media Influence Model Based on Spillover Effects of Attitude Toward Genetically Modified Food
Authors: Ho Shirley S , Goh Tong Jee , Chuah Agnes S F , Leung Yan Wah , Bekalu Mesfin Awoke , Viswanath Kasisomayajula 
Published: July 2020

AbstractInformed by the notion of spillover effects between two phenomena, this study examines how differences in prior attitudes would influence the relationships posited by the influence of presumed media influence (IPMI) model. Specifically, this study examines how pre-existing favorable and unfavorable attitudes toward genetically modified (GM) food are associated with audiences’ intention to consume nano-enabled food. The results of a nationally representative survey with 1,000 respondents found general support for the IPMI from media attention to behavioral intentions, through attitude and social norms. Further, a multigroup analysis of the IPMI provided evidence for differences in the IPMI effects between the audiences with favorable and unfavorable pre-existing attitudes toward GM food. These results contribute to a stronger theoretical understanding of the IPMI in terms of how pre-existing attitudes toward a preceding food technology can have a spillover effect on how audiences make decisions regarding a newer food technology.
The Politics of Legitimation in International Organizations
Authors: Binder Martin , Heupel Monika 
Published: July 2020

AbstractTo govern effectively, international organizations (IOs) crucially depend on legitimation and support from their member states. But which states claim legitimacy for IOs, which challenge their legitimacy, and why? We address this gap in the literature by analyzing the legitimation strategies that states use in institutionalized discursive spaces within IOs. Specifically, we examine how United Nations (UN) member states seek to legitimate or delegitimate the UN Security Council in public debates in the UN General Assembly. We formulate a set of hypotheses that link specific state characteristics to evaluative statements on the Council’s legitimacy. We test these hypotheses on an original dataset using a non-linear regression model. In line with our theoretical expectations, we find that legitimation strategies are driven by a state’s membership of the Council and by its attitudes towards the United States. Contrary to our theoretical expectations, economically powerful states and states that are willing to delegate authority to supranational organizations are more likely to challenge the Council’s legitimacy. Furthermore, we provide evidence that states’ legitimacy claims resonate among fellow states, that is, among the Council’s primary audience. More generally, our findings suggest that making public claims about the Security Council’s legitimacy is not an empty diplomatic exercise, and that states do not make these claims at random. Legitimation strategies follow discernible patterns that can be explained by specific state characteristics.
Bartholin’s gland hyperplasia with dysplastic changes: a rare case report
Authors: Yahaya James J 
Published: July 2020

AbstractThe purpose of this paper is to report unusual, rarest and interesting case of a patient with nodular hyperplasia of Bartholin's gland with dysplastic changes. The case of a 30-year old female with right-sided Bartholin’s gland hyperplasia with dysplastic changes, which was confirmed histopathologically, is presented in this paper. The patient reported increased swelling of the right major labium when she became sexually aroused with intermittent dyspareunia during intercourse. Surgical excision of the swelling was done under general anaesthesia. Healing of the surgical scar was complete and she reported no any discomfort or dyspareunia during sexual intercourse. Bartholin’s gland swellings with firm consistency require complete excision due to possibility of being neoplastic rather than just inflammation, cyst or obstructed gland particularly in postmenopausal women. Diagnosis of nodular hyperplasia of Bartholin’s gland with areas of dysplastic changes in the present case is of great interest and requires further investigation.
Dormancy cycling is accompanied by changes in ABA sensitivity in Polygonum aviculare seeds
Authors: Laspina Natalia Verónica , Batlla Diego , Benech-Arnold Roberto Luis 
Published: July 2020

Abstract
Polygonum aviculare seeds show high levels of primary dormancy (PD). Low winter temperatures alleviate dormancy and high spring temperatures induce seeds into secondary dormancy (SD), naturally establishing stable seedbanks cycling through years. The objective of this work was to elucidate the mechanism(s) involved in PD expression and release, and in SD induction in these seeds, and the extent to which abscisic acid (ABA) and gibberellins (GAs) are part of these mechanisms. Quantification of endogenous ABA both prior to and during incubation, and sensitivity to ABA and GAs, were assessed in seeds with contrasting dormancy. Expression analysis was performed for candidate genes involved in hormone metabolism and signaling. It was found that endogenous ABA content does not explain either dormancy release or dormancy induction; moreover, it does not seem to play a role in dormancy maintenance. However, dormancy modifications were commonly accompanied by changes in ABA sensitivity. Concomitantly, induction into SD, but not PD, was characterized by a increased PaABI-5 and PaPYL transcription, and a rise in GA sensitivity as a possible counterbalance effect. These results suggest that dormancy cycling in this species is related to changes in embryo sensitivity to ABA; however, this sensitivity appears to be controlled by different molecular mechanisms in primary and secondary dormant seeds.
Contact Lens Wear, Corneal Complications, and U.S. Service Member Readiness
Authors: Flanagan Gerald , Velez Tom , Gu Weidong , Singman Eric 
Published: July 2020

Abstract

Introduction
Ulcerative keratitis (UK), or corneal ulcer, is a sight-threatening and readiness-lowering medical condition that begins with a corneal infiltrative event (CIE). Contact lens (CL) wear poses a particular risk for a CIE and therefore is restricted for most active duty service members (SMs). In this study, we explored a large Department of Defense/Veterans Affairs (DoD/VA) database to estimate the prevalence of UK and CIE and their association with CL wear.

Materials and Methods
The DoD/VA Defense and Veterans Eye Injury Vision Registry, an initiative of the DoD/VA Vision Center of Excellence, was explored using natural language processing software to search for words and diagnostic codes that might identify cornea injuries and CL wear. The effect of UK and CIE on readiness was explored by evaluating the duration between the first and final visits noted in the database.

Results
A total of 213 UK cases were identified among the 27,402 SMs for whom data were recorded in Defense and Veterans Eye Injury Vision Registry. The odds ratios of UK and CIE being associated with CL wear were 13.34 and 2.20, respectively. A less specific code (superficial corneal injury) was found to be the most commonly used diagnosis in the database, and the odds ratio of CL wearers having that diagnosis was 2.25. CL-wearing patients with corneal disease also required more clinic encounters than those who did not wear CLs.

Conclusions
This study supports the current restriction on CL wear among nonpilot active duty SMs and quantifies the significantly enhanced risk of developing corneal ulcers posed by that habit.
Developing molecular-level models for organic field-effect transistors
Authors: Li Haoyuan , Brédas Jean-Luc 
Published: July 2020

Abstract
Organic field-effect transistors (OFETs) are not only functional devices but also represent an important tool for measuring the charge-transport properties of organic semiconductors (OSs). Thus, efforts to understand the performance and characteristics of OFET devices are not only useful in helping achieve higher device efficiencies but also critical to ensuring accuracy in the evaluations of OS charge mobilities. These studies rely on OFET device models, which connect the measured current characteristics to the properties of the OSs. Developing such OFET models requires good knowledge of the charge-transport processes in OSs. In device active layers, the OS thin films are either amorphous (e.g. in organic light-emitting diodes and organic solar cells) or crystalline (e.g. those optimized for charge transport in OFETs). When the electronic couplings between adjacent OS molecules or polymer chain segments are weak, the charge-transport mechanism is dominated by hopping processes, which is the context in which we frame the discussion in this Review. Factors such as disorder, mobility anisotropy, traps, grain boundaries or film morphology all impact charge transport. To take these features fully into account in an OFET device model requires considering a nano-scale, molecular-level resolution. Here, we discuss the recent development of such molecular-resolution OFET models based on a kinetic Monte Carlo approach relevant to the hopping regime. We also briefly describe the applicability of these models to high-mobility OFETs, where we underline the need to extend them to incorporate aspects related to charge delocalization.