bluuebunny/crossref_metadata_2025_split huggingface dataset:
The gaps in health-adjusted life years (HALE) by income and region in Korea: a national representative bigdata analysis
Authors: Yoon Seok-Jun , Kim Young-Eun , Ock Minsu , Park Hyesook , Kim Ki-beom , Go Dun-Sol , Jung Yoon-Sun 
Published: July 2020

Abstract
Background: This study aims to calculate the health-adjusted life years (HALE) by using years lived with disability from the national claims data and aims to identify the differences and inequalities in income level and region. Methods: The study was carried out on total population receiving health insurance and medical benefits. We calculated incidence-based years lived with disability (YLD) for 260 disease groups, and this was used as the number of healthy years lost to calculate HALE. We adopted the insurance premium to calculate the income as a proxy indicator. For the region classification, we chose 250 administrative districts to cover the whole country.Results: The primary outcome was HALE in the Korean population. The second outcome was the HALE’s gap in terms of income and region. HALE increased from 2008 to 2016. Results by gender suggest that HALE in males increases faster than in females. Results by income level show that HALE is higher in higher income level. In 2016, the gap in HALE between Q1 and Q2, the lower income group, was about 5.10 years. The gap in females by income level was smaller than that in males, which can be because males have greater inequality in terms of HALE by income level. Moreover, the gap in HALE by region was found to increase.Conclusions: Results suggest that there is an inequality in years of living with disability in terms of income level in Korea. Therefore, we need intensive management for the low-income group to promote HALE at the national level.
Identification of novel loci and candidate genes for cucumber downy mildew resistance using GWAS
Authors: Liu Xiaoping , Lu Hongwei , Liu Panna , Miao Han , Yan Zifu , Bai Yuling , Gu Xingfang , Zhang Shengping 
Published: July 2020

Abstract

Background
Downy mildew (DM) is one of the most serious diseases in cucumber and brings the loss of yield and profit. Multiple QTLs for DM resistance have been detected, however, no loci related to resistance was reported using genome-wide association analysis (GWAS). In this study, the core germplasm (CG) of cucumber lines that had been constructed and resequenced were used to identify DM resistance Loci using GWAS technology.
Results
Thirteen loci (dmG1.1, dmG1.2, dmG2.1, dmG2.2, dmG3.1, dmG4.1, dmG4.2, dmG5.1, dmG5.2, dmG6.1, dmG6.2, dmG7.1 and dmG7.2) associated with DM resistance were detected on all the seven chromosomes. Among these loci, dmG2.1 and dmG7.1 were novel loci compared with previous studies. Based on the annotation of homologous genes in Arabidopsis and pairwise LD correlations, Csa1G575030 could be the most likely candidate gene of dmG1.2; Csa2G059820 and Csa2G060360 could be the candidate gene of dmG2.1. A WRKY transcription factor Csa5G606470 could be the candidate gene of dmG5.2. Csa7G004020 could be the candidate gene of dmG7.1.
Conclusions
These results identify five candidate genes for four loci related to DM resistance in cucumber which provide theoretical basis for gene cloning and genetic breeding of DM resistance in cucumber.
WITHDRAWN: Rhizosphere Microbiome is Associated with Yield and Medical Value of the Perennial Panax Notoginseng
Authors: Wei Guangfei , Li Mengzhi , Zhang Guozhuang , Chen Zhongjian , Wei Fugang , Jiao Shuo , Qian Jun , Wang Yong , Meng Xiangxiao , Yu Yuqi , Dong Linlin , Chen Shilin 
Published: July 2020

Abstract
Background: Rhizosphere microbiome play important roles in promoting plant growth. However, it is not well understood how rhizosphere microbiome were driven by medical plants during growth stages and whether they contribute the accumulation of medical values. Panax notoginseng is a perennial medicinal plant, which belowground biomass and saponin contents are the important indicators of its value. Here, we use high-throughput sequencing method to study the temporal dynamics of perennial P. notoginseng rhizosphere microbiomes and the relationship between the indicators and core rhizosphere microbiomes.Results: The results show that the diversity, composition and network structures of the bacterial and fungal communities are mainly driven by the developmental stages. And succession characteristics of bacterial and fungal diversity show similar parabolic patterns during the developmental stages. Enrichment and depletion of the bacterial and fungal communities are active at the 3-year root growth (3YR) stage. From samples collected at a large-spatial P. notoginseng production area at the 3YR stage, we obtained 639 bacterial and 310 fungal core operational taxonomic units (OTUs). Analysis of the data indicate that the microbiome diversity is related to the belowground biomass and total saponin contents. Some genera, such as Pseudomonas, Massilia, Sphingobium, and Phoma are positively correlated to the belowground biomass, and genera likely Staphylotrichum, Chaetosphaeria, and Podospora are positively correlated with total saponin contents. Additionally, we identified 36 microbial functions involving in plant-microbe and microbe-microbe interactions, nutrition acquisition, and disease resistance. They are related to belowground biomass and saponin contents. Conclusions: In short, this study provides a detailed and systematic survey of rhizosphere microbiome in P. notoginseng and reveals that P. notoginseng rhizosphere microbiomes are largely driven by the developmental stages, while the core microbiomes are related to the belowground biomass and saponins contents of the plant. The finding may enhance our understanding of the interaction between microbes and perennial plants and improve our ability to manage root microbiota for sustainable production of the herb medicine.
Targeting Tumor Microenvironment: Metformin Suppresses IL-22 Induced Hepatocellular Carcinoma by Upregulating Hippo Signalling Pathway.
Authors: Zhao Dong , Zhou Tao , Luo Yi , Wang Chenchen , Xu Dongwei , Zhong Chengpeng , Zhang Jianjun , Xia Qiang 
Published: July 2020

Abstract
Background: Epidemiological studies have shown direct associations between type 2 diabetes and the risk of cancers. Accumulating evidence indicates that metformin is profoundly implicated in preventing tumor development. However, the exact mechanism underlying the anti-tumor effects of metformin in hepatocellular carcinoma (HCC) is still not clear. Methods: In this study, we investigated the effects of metformin on a mouse hepatocellular carcinoma (HCC) model and interleukin-22 (IL-22)-associated carcinogenesis in vitro. Results: We found that metformin significantly suppressed the incidence and tumor burden of HCC in the diethyl-nitrosamine (DEN)-induced HCC mouse model. As expected, expression of IL-22, an important factor involved in HCC progression, was markedly reduced by metformin. Treatment of HCC cells with metformin inhibited IL-22 induced cell proliferation, migration and invasion, and promoted cell apoptosis. Furthermore, ectopic expression of IL-22 makes HCC more aggressive whereas metformin largely compromised it in vitro and in vivo. Mechanistically, the whole transcriptome analysis and functional analysis revealed that Hippo signaling pathway was involved in the anti-tumor ability of metformin. Consistent with this, metformin directly activated Mst1/2, phosphorylated YAP1 in vitro. After blocking Hippo pathway by XMU-MP-1, the inhibitor of MST1/2, the inhibitory effects by metformin were dramatically attenuated as shown by in vitro study. Conclusions: Collectively, our findings illuminate a new regulatory mechanism, metformin activates Hippo signaling pathway to regulate IL-22 mediated HCC progression and provide new insights into its tumor-suppressive roles.
Predictive Value of ERCC1 mRNA Level and Pretreatment EBV-DNA Virus Load in Stage II Nasopharyngeal Carcinoma Patients Receiving Intensity-Modulated Radiotherapy with Concurrent Cisplatin
Authors: Hua Li , Chen Shaojun , Wei Mengzhuan , Shen Yongqi , Long Jianxin , Lin Zhan , Meng Yiliang , Yin Ping , huang haixin , Tu Xiaoning , Yao Min 
Published: July 2020

Abstract
Background: The protein expression of ERCC1 in DNA repair genes was related to resistance platinum and predicting treatment outcomes in various malignant carcinoma ,the level of plasma Epstein-Barr virus（EBV）DNA concentrations is positively correlated with clinical stages of nasopharyngeal carcinoma(NPC), but the predictive value of ERCC1 mRNA and EBV-DNA level for stratified treatment with stage II NPC is unclear precisely. This study aimed to assess the predictive value of combined EBV-DNA and ERCC1 in stage II NPC patients treated with intensity-modulated radiotherapy (IMRT) with concurrent cisplatin and provide guidance for future stratified treatment.Methods: A total 78 stage II NPC patients who received IMRT and concurrent cisplatin-based chemotherapy had measurements of ERCC1 mRNA and pre-treatment EBV DNA levels by real-time PCR (RT-PCR) analysis were analyzed. Associations of ERCC1 mRNA and pre-treatment EBV DNA levels with clinical characteristics and survivals were evaluated.Results: Cut-off value of ERCC1 mRNA obtained from ROC curve was used and there were significant differences in progression-free survival (PFS) and overall survival (OS) between high expression group as compared to low expression group (P=0.021 and 0.030, respectively). Patients with pretreatment EBV-DNA<2000 copies/ml had significantly better PFS (P= 0.024) than those with pretreatment EBV-DNA≥2000 copies/ml, but there was no significant difference in OS (P= 0.062). Patients were divided into three groups by combination of ERCC1 mRNA and EBV-DNA level ERCC1 mRNA low expression/pre EBV-DNA<2000 copies/ml, ERCC1 mRNA low expression/pre EBV-DNA≥2000 copies/ml, ERCC1 mRNA high expression/pre EBV-DNA≥2000 copies/ml. In these groups, 1-year, 3-year, 5‐year OS were 100%, 100%, 100%; 100%, 94.1%, 90.9%; 100%, 85%, 72.9%, respectively (P=0.038); 1-year, 3-year, 5‐year PFS were 100%, 100%, 100%; 97.1%, 91.2%, 84.8%; 95%, 85%, 71.4%, respectively (P=0.028). Multivariate analysis showed combination of ERCC1 mRNA and EBV-DNA levels remained independent prognostic factor but not ERCC1 mRNA and EBV-DNA alone. Conclusion: Combined ERCC1 mRNA and pre EBV-DNA is a better prognostic factor in stage II NPC patients treated with concurrent chemoradiation. Patients with ERCC1 mRNA high expression/pre EBV-DNA≥2000 copies/ml should be treated with more aggressive regimen.
lncRNA-miRNA-mRNA ceRNA Network in Thyroid Carcinoma from The Cancer Genome Atlas
Authors: Mo Guoheng , Jiang Qunguang , Wang Zixuan , Zheng Zhaoting , Chen XiaoSi 
Published: July 2020

Abstract
Increasing evidence indicates that the competitive endogenous RNA (ceRNA) hypothesis, that is, long non-coding RNA (LncRNA) can competitively bind microRNA (miRNA) through miRNA response elements to affect the expression of target RNA, and dysregulation of LncRNA expression plays a key role in tumor progression. The papillary thyroid carcinoma that we studied is the most significant pathological type of thyroid cancer, but its ceRNA network has not been extensively evaluated. We analyzed level-3 data from RNA-Seq of 58 para-carcinoma tissues and 501 patients with primary papillary thyroid carcinoma (PTC) using the DEseq software package and downloaded clinical information from The Cancer Genome Atlas (TCGA) to find potential biomarkers or therapeutic targets. As a result, 149 differential miRNAs were selected, including 117 up -regulated, 32 down-regulated, and 3099 differential mRNAs, including 1976 up-regulated, 1123 down-regulated, and 434 differential lncRNAs, including 331 up-regulated and 103 down-regulated (Fold Change > 2, P < 0.05). The interactions between these differentially expressed RNA groups constitute the ceRNA network of PTC. Moreover, we used the microde database to predict the miRNAs that may be acted by the above screened differential lncRNAs and intersected with the selected miRNAs, and further predicted the target genes of the intersecting miRNAs by TargetScan, miRTarBase and miRDB, and intersected with the selected mRNAs. From the constructed ceRNA network we can see that Linc00460 may cause the invasion and metastasis of PTC by competitively inhibiting hsa-mir-150 and upregulating the expression of its downstream target gene EREG. Our study identified a series of lncRNAs associated with PTC progression and prognosis, and this complex ceRNA interaction network provides guidance for better understanding of the molecular mechanisms of PTC and can be used as an effective diagnostic tool for PTC invasion, metastasis and prognosis. Kaplan-Meier analysis of the differentially expressed RNAs associated with PTC pathogenesis confirmed that the lncRNAs AC097717.1, C20orf203, EMX2OS were potentially associated with the prognosis of PTC (P<0.05).
Socioeconomic and behavioural factors associated with access to and use of patient Electronic Health Records: a cross-sectional analysis of four European countries.
Authors: Paccoud Ivana , Baumann Michèle , Bihan Etienne Le , Pétré Benoît , Breinbauer Mareike , Böhme Philip , Chauvel Louis , Leist Anja K. 
Published: July 2020

Abstract
Background: Access to and use of digital technology are more common among people of higher socioeconomic status. These differences might be due to a lack of interest, not having physical access or having lower intentions to use this technology. By integrating the digital divide approach and the User Acceptance of Information Technology (UTAUT) model, this study aims to further our understanding of socioeconomic factors and the mechanisms linked to different stages in the use of individuals’ electronic health records (EHR): motivations, intentions and physical access to patients EHR.Methods: A cross-sectional online and in-person survey was undertaken in the areas of Lorraine (France), Luxembourg, Rhineland-Palatinate and Saarland (Germany), and Wallonia (Belgium). First, exploratory factor analysis was performed to group items derived from the UTAUT model. Next, we applied linear and logistic regressions controlling for country-level heterogeneity, health and sociodemographic factors.Results: A total of 829 individuals aged over 18 completed the questionnaire. We found evidence of socioeconomic inequalities in the access to and use of EHR. Education and income played a significant role in individual’s desire to access their EHR. Being older than 65 years, and migrant, were negatively related to the desire to access EHR. An income gradient was found in having a physical access to EHR, while for the subgroup of respondents who expressed desire to have access, higher educational level was positively associated with intentions to regularly use EHR. In fully adjusted model testing the contribution of UTAUT-derived factors, individuals who perceived EHRs to be useful and had the necessary digital skills were more inclined to use their EHR regularly. Social support and lack of anxiety in using technology were strong predictors of regular use of EHR.Conclusion: The findings highlight the importance of considering all stages in the use of EHR (desire, physical access, and regular use), while paying special attention to migrants and people with lower socioeconomic backgrounds who may not be able to exploit the potential of EHRs. As EHR use is expected to come with health benefits, facilitating access and regular use for those less inclined could lead to increases in health equity.
Clinical characteristics and related factors of fungal empyema thoracis
Authors: Zhong EnJian , Qian HuiWen , Hou ChunQi , Li Juan , Qian XueFeng , Zou LiRong , Li Jing , Zhang YongHong , Zhong ChongKe , ji cheng 
Published: July 2020

Abstract
ObjectData on the characteristics and related factors of fungal empyema thoracis (FET) are limited. Our aim is to investigate the clinical characteristics and related factors of FET.MethodsWe conducted a retrospective study of patients with positive culture from pleural effusion who were admitted to the First Affiliated Hospital of Soochow University between January 2007 and January 2018. The clinical and laboratory characteristics of all study participants were collected. Logistic regression models were used to assess the related factors of FET.ResultsThere were 30 patients diagnosed with FET. The median age of patients with FET was 62.7 years old and 24 (80.0%) were male. The most frequent pathogens of FET were Candida albicans (55.9%). Diabetes mellitus (23.3% vs. 7.9%, P = 0.019) and upper gastrointestinal tract perforation or rupture (20.0% vs. 2.1%, P < 0.001) were associated with increased risk of FET. The adjusted odds ratios (95% confident interval) were 3.11 (1.02–9.56) for diabetes mellitus and 12.71 (3.47–46.55) for upper gastrointestinal tract perforation or rupture. There were 10 deaths (33.3%) among FET patients after one-year of follow-up.ConclusionsDiabetes mellitus and upper gastrointestinal tract perforation or rupture were associated with increased risk of FET.
Effects of pre-workout multi-ingredient supplement on anaerobic performance: randomized double-blind crossover study
Authors: Kaczka Piotr , Batra Amit , Kubicka Katarzyna , Maciejczyk Marcin , Rzeszutko-Bełzowska Agata , Pezdan-Śliż Iwona , Michałowska-Sawczyn Monika , Przydział Marta , Płonka Artur , Cięszczyk Paweł , Humińska-Lisowska Kinga , Zając Tomasz 
Published: July 2020

Abstract
Background: The purpose of this study was to investigate the acute effects of commercially available pre-workout supplement Knockout 2.0 ® on anaerobic performance in resistance trained men.Methods: Twenty-three men underwent three testing sessions administrated in a randomized and double-blind fashion separated by a seven-day break. The participants performed three exercise tests: isokinetic strength test, maximal strength test and Wingate test. Statistical analysis was conducted in R environment. Linear mixed models were estimated via R package lme4.Results: The mean knee peak torque was significantly greater in supplemented group for right and left knee flexors (placebo: 103.17 ± 37.61 Nm, and supplemented group: 131.84 ± 29.31 Nm where p=0.001, and placebo: 103.72 ± 39.35, and supplemented group: 129.38 ± 28.44, where p=0.001; respectively) as well as for right and left knee extensors (placebo: 202.65 ± 58.64 Nm, and supplemented group: 237.22 ± 54.75 Nm where p=0.001, and placebo: 203.27 ± 63.2 Nm versus supplemented group: 229.84 ± 50.8 Nm where p=0.002; respectively).The significant difference was observed in mean anaerobic power between supplemented and placebo group for right and left knee flexors (p=0.002 and p=0.005, respectively) as well as for right and left knee extensors (p=0.001 and p=0.002; respectively).There was also observed that the time to peak torque was significantly greater in supplemented group for right and left knee flexors (p=0.002 for both legs). The significant difference was also observed in mean power between supplemented and placebo group during Wingate test (placebo: 8.49 ± 0.57 W/kg, and supplemented group: 8.66 ± 0.55 W/kg where p=0.038). Moreover the mean 3-RM strength test was significantly greater in supplemented group with p=0.001.Conclusions: The results of the study indicate that Knockout 2.0® significantly improves upper and lower body strength and power output in resistance trained men.
Insomnia Symptoms in Chinese Patients with Chronic Schizophrenia: Prevalence, Clinical Correlates and Relationship with Inflammation
Authors: Xia Lei , Zhong Yi , Liu Zhiwei , Zhang Yulong , Li Wenzheng , Wang Yun , Tao Degang , Zhang Kai , Liu Huanzhong , Zhang Xiang Yang 
Published: July 2020

Abstract
Background: Sleep disturbances are common in patients with schizophrenia, with serious consequences. The purpose of this study was to investigate the prevalence and clinical correlates of insomnia symptoms, and to explore the relationship between insomnia and inflammatory markers in Chinese patients with chronic schizophrenia. Methods: A total of 328 inpatients with chronic schizophrenia were recruited. Insomnia Severity Index (ISI), Calgary Depression Scale for Schizophrenia (CDSS), and Positive and Negative Syndrome Scale (PANSS) were used to assess the severity of insomnia, depression, and psychotic symptoms. The plasma levels of several inflammatory markers (CRP, IL-6, and TNF-α) were measured.Results: The prevalence of insomnia symptoms in patients with schizophrenia was 38.4%. Depressive symptoms were significantly associated with insomnia symptoms (OR = 1.23, 95%CI: 1.13-1.33, P < 0.001). Higher CDSS score (beta = 0.55, t = 8.21, P < 0.001) and older age (beta = 0.06, t = 3.59, P < 0.001) were significantly associated with higher ISI score, while taking a single SGA (beta = -0.85, t = -1.99, P < 0.05) was independently associated with lower ISI score. There was no significant association between any inflammatory markers and insomnia or ISI score.Conclusions: Our results demonstrate that the prevalence of insomnia symptoms is high in Chinese inpatients with chronic schizophrenia. Some demographic and clinical variables, such as depressive symptoms and older age, are risk factors, while others are beneficial factor, such as taking atypical antipsychotic drug for insomnia in schizophrenia patients. No association has been found between insomnia symptoms and inflammation.
Flash Flood Management Approach for Flood Risk Areas Using Numerical Climate Modeling Data Analysis
Authors: Hossain Md. Altaf , Ishaque Fahmida , RAHMAN MD HAFIZUR , Uddin Gazi Tamiz , Sarker Md. Abdur Rashid , Baidya Joyshankar , Ripa Israt Jahan 
Published: July 2020

Abstract
The present study measures management of flash flood to avoid the devastating impacts on boro rice production at Sunamganj district. This study interacts for meteorological parameter assessment with global climate data. Three major climatic parameters (precipitation, temperature and cloud fraction) have been considered for this study. These three climatic data were analyzed using numerical software EdGCM for the period 1920 to 2020 and then downscaled by Transform software. Flash floods have been classified as general flash floods and devastating flash flood. Flash floods have been reported to be severe in March and April. A trend was evaluated for the detection of flash flooding occurrences. The study concludes that when temperature is high, a small amount of rainfall can cause a slight cloud fraction to cause flooding. Flash flooding occurs when temperature is above 76oF(24.44oC), rainfall is at least 3 mm and cloud fraction is at most 46%. Furthermore, for validation Mann-Kendall trend analysis have been done. The test result discovered increasing trend for rainfall and decreasing trend for temperature and cloud fraction. Finally, an algorithm was developed in C++ program as a flash flood precaution tool which helped to prepare strategy as well as to adapt with the flash flood.