bluuebunny/crossref_metadata_2025_split huggingface dataset:
Single‐Cell Transcriptomics of Engineered Cardiac Tissues From Patient‐Specific Induced Pluripotent Stem Cell–Derived Cardiomyocytes Reveals Abnormal Developmental Trajectory and Intrinsic Contractile Defects in Hypoplastic Right Heart Syndrome
Authors: Lam Yin‐Yu , Keung Wendy , Chan Chun‐Ho , Geng Lin , Wong Nicodemus , Brenière‐Letuffe David , Li Ronald A. , Cheung Yiu‐Fai 
Published: October 2020

Background
To understand the intrinsic cardiac developmental and functional abnormalities in pulmonary atresia with intact ventricular septum (PAIVS) free from effects secondary to anatomic defects, we performed and compared single‐cell transcriptomic and phenotypic analyses of patient‐ and healthy subject–derived human‐induced pluripotent stem cell–derived cardiomyocytes (hiPSC‐CMs) and engineered tissue models.

Methods and Results
We derived hiPSC lines from 3 patients with PAIVS and 3 healthy subjects and differentiated them into hiPSC‐CMs, which were then bioengineered into the human cardiac anisotropic sheet and human cardiac tissue strip custom‐designed for electrophysiological and contractile assessments, respectively. Single‐cell RNA sequencing (scRNA‐seq) of hiPSC‐CMs, human cardiac anisotropic sheet, and human cardiac tissue strip was performed to examine the transcriptomic basis for any phenotypic abnormalities using pseudotime and differential expression analyses. Through pseudotime analysis, we demonstrated that bioengineered tissue constructs provide pro‐maturational cues to hiPSC‐CMs, although the maturation and development were attenuated in PAIVS hiPSC‐CMs. Furthermore, reduced contractility and prolonged contractile kinetics were observed with PAIVS human cardiac tissue strips. Consistently, single‐cell RNA sequencing of PAIVS human cardiac tissue strips and hiPSC‐CMs exhibited diminished expression of cardiac contractile apparatus genes. By contrast, electrophysiological aberrancies were absent in PAIVS human cardiac anisotropic sheets.

Conclusions
Our findings were the first to reveal intrinsic abnormalities of cardiomyocyte development and function in PAIVS free from secondary effects. We conclude that hiPSC‐derived engineered tissues offer a unique method for studying primary cardiac abnormalities and uncovering pathogenic mechanisms that underlie sporadic congenital heart diseases.
Identification of Key Small Non‐Coding MicroRNAs Controlling Pacemaker Mechanisms in the Human Sinus Node
Authors: Petkova Maria , Atkinson Andrew J. , Yanni Joseph , Stuart Luke , Aminu Abimbola J. , Ivanova Alexandra D. , Pustovit Ksenia B. , Geragthy Connor , Feather Amy , Li Ning , Zhang Yu , Oceandy Delvac , Perde Filip , Molenaar Peter , D’Souza Alicia , Fedorov Vadim V. , Dobrzynski Halina 
Published: October 2020

Background

The sinus node (SN) is the primary pacemaker of the heart. SN myocytes possess distinctive action potential morphology with spontaneous diastolic depolarization because of a unique expression of ion channels and Ca
2+
‐handling proteins. MicroRNAs (miRs) inhibit gene expression. The role of miRs in controlling the expression of genes responsible for human SN pacemaking and conduction has not been explored. The aim of this study was to determine miR expression profile of the human SN as compared with that of non‐pacemaker atrial muscle.

Methods and Results

SN and atrial muscle biopsies were obtained from donor or post‐mortem hearts (n=10), histology/immunolabeling were used to characterize the tissues, TaqMan Human MicroRNA Arrays were used to measure 754 miRs, Ingenuity Pathway Analysis was used to identify miRs controlling SN pacemaker gene expression. Eighteen miRs were significantly more and 48 significantly less abundant in the SN than atrial muscle. The most interesting miR was miR‐486‐3p predicted to inhibit expression of pacemaking channels: HCN1 (hyperpolarization‐activated cyclic nucleotide‐gated 1), HCN4, voltage‐gated calcium channel (Ca
v
)1.3, and Ca
v
3.1. A luciferase reporter gene assay confirmed that miR‐486‐3p can control HCN4 expression via its 3′ untranslated region. In
ex vivo
SN preparations, transfection with miR‐486‐3p reduced the beating rate by ≈35±5% (
P
<0.05) and HCN4 expression (
P
<0.05).

Conclusions
The human SN possesses a unique pattern of expression of miRs predicted to target functionally important genes. miR‐486‐3p has an important role in SN pacemaker activity by targeting HCN4, making it a potential target for therapeutic treatment of SN disease such as sinus tachycardia.
N‐Terminal Pro Brain, N‐Terminal Pro Atrial Natriuretic Peptides, and Dynamic Cerebral Autoregulation
Authors: Mahinrad Simin , Sabayan Behnam , Garner Chaney R. , Lloyd‐Jones Donald M. , Sorond Farzaneh A. 
Published: October 2020

Background
Elevated natriuretic peptides (NP) are associated with adverse cerebrovascular conditions including stroke, cerebral small vessel disease, and dementia. However, the mechanisms underlying these associations remain unclear. In this study, we examined the relationship of NT‐proBNP (N‐terminal pro brain NP) and NT‐proANP (N‐terminal pro atrial NP) with cerebrovascular function, measured by cerebral autoregulation.

Methods and Results
We included 154 participants (mean age 56±4 years old) from the CARDIA (Coronary Artery Risk Development in Young Adults) cohort. NT‐proBNP and NT‐proANP were measured in blood samples from the year 25 examination using electrochemiluminescence Immunoassay and enzyme‐linked immunoassay, respectively. Dynamic cerebral autoregulation (dCA) was assessed at the year 30 examination by transcranial Doppler ultrasound, using transfer function analysis (phase and gain) of spontaneous blood pressure and flow velocity oscillations, where lower phase and higher gain reflect less efficient cerebral autoregulation. We used multivariable linear regression models adjusted for demographics, vascular risk factors, and history of kidney and cardiac diseases. Higher NT‐proBNP levels at year 25 were associated with lower phase (β [95% CI]=−5.30 lower degrees of phase [−10.05 to −0.54]) and higher gain (β [95% CI]=0.06 higher cm/s per mm Hg of gain [0.004–0.12]) at year 30. Similarly, higher NT‐proANP levels were associated with lower phase (β [95% CI]=−9.08 lower degrees of phase [−16.46 to −1.70]).

Conclusions
Higher circulating levels of NT‐proBNP and NT‐proANP are associated with less efficient dCA 5 years later. These findings link circulating NP to cerebral autoregulation and may be one mechanism tying NP to adverse cerebrovascular outcomes.
The Ascent of the Soul as Spiritual Exercise in Plotinus’ Enneads
Authors: Stróżyński Mateusz 
Published: October 2020

Abstract
The purpose of the article is to demonstrate that the ascent of the soul as one of the fundamental spiritual exercises in Plotinus’ philosophy can be approached from three perspectives: anabatic proper, aphaeretic and agnoetic. All of them are based on the hierarchical structure of knowledge and being in Plotinus’ philosophy, but they differ in details. The methods are reconstructed on the basis of the analysis of selected passages from the Enneads.
Arrian’s Ektaxis in the Roman Empire
Authors: Chiritoiu Daniel 
Published: October 2020

Abstract
This paper proposes to discuss one of Arrian’s so-called ‘minor texts’—the Acies contra Alanos which I prefer to call Ektaxis kata Alanōn—and show how it is not only a military manual, but also allows Arrian to discuss identity, fitting into broader patterns and discourses of the Second Sophistic. In the Ektaxis Arrian, much like a sophist, creates different personas and layers of identity not just for himself but also for his troops, and takes the opportunity to present the Roman army in a diverse way, which differs from portrayals in other authors.
Trojan Restoration and the Aeneid in Horace, Odes 3.3
Authors: Wallis Jonathan 
Published: October 2020

Abstract
This article argues that Juno’s speech in Horace’s Odes 3.3 includes a short series of programmatic allusions to Virgil’s Aeneid that assist Horace in promoting the distinct identity of his own lyric poetry. Juno’s speech asserts that Rome’s passage to greatness depends on not ‘rebuilding Troy’. Horace’s allusions identify the motif of Trojan restoration as a central theme in the Aeneid’s narrative, and, in a metapoetic sense, associate it pejoratively with the cultural performance of the epic itself in its canonical retelling of the Trojan story. In this way Horace uses Juno’s speech strategically to characterise the Aeneid as decadent and regressive; by contrast Juno promotes moral restraint as a virtue that characterises Horatian lyric.
Prudentius’ Agnes and the Elegiac puella
Authors: Tsartsidis Thomas 
Published: October 2020

Abstract
In Peristephanon 14, Prudentius creates an inventive verse rendering of the martyrdom of Agnes. Interestingly, in this poem, the portrayal of Agnes shares many features with the elegiac puellae of Roman love elegy. Prudentius’ classicising poetry is characterised by the mixture of genres and literary traditions, one of them being Roman love elegy. The affinities, however, between Prudentius and the latter tradition deserve closer attention. In this paper, by identifying vocabulary, themes and motifs of Roman elegy in Peristephanon 14, I will illustrate ways in which Prudentius’ Agnes can be read as a Christianised elegiac puella.
Some new and known species of Bomolochidae (Copepoda, Cyclopoida) parasitic on Japanese actinopterygian fishes, 1 — with discussion on sexually dimorphic features in the family
Authors: Izawa Kunihiko 
Published: October 2020

Abstract
Three new and five known species of Bomolochidae Claus, 1875, parasitic on Japanese actinopterygian fishes, are (re)described. They are Bomolochus bellones Burmeister, 1835, B. decapteri Yamaguti, 1936, B. hoi sp. nov., Naricolax sphyraenae sp. nov., Nothobomolochus cypseluri (Yamaguti, 1953), Orbitacolax brevispinus Moon, Choi & Venmathi Maran, 2018, O. scombropsi sp. nov., and O. unguifer Kim & Moon, 2013. The copepodid IV female of B. bellones, the male and copepodid IV female of Na. sphyraenae, the male and copepodid IV and V female of No. cypseluri and O. unguifer are herein described. Sexual dimorphism is found in the antennule, maxilliped, and legs in the family.
Towards the rebuilding of spiny lobster stocks in Brazil: a review
Authors: Cruz Raúl , Santana João V. M. , Barreto Carlos G. , Borda Carlos A. , Torres Marina T. , Gaeta Juliana C. , Da Silva Jessica L. S. , Saraiva Soniamar Z. R. , Salazar Ivo S. O. , Cintra Israel H. A. 
Published: October 2020

Abstract
In this study, we used the Monte Carlo model (CMSY) to estimate fisheries reference points based on annual lobster landings. During the period 2012-2018, fishing pressure on lobster stocks decreased and a tendency for recovery was observed, despite the low biomass levels of approximately 29%. In 2019, the stock size was capable of producing yields close to the maximum sustainable yield (5770 t), with a probability of 58.1%. The enforcement of management measures should be continued in order to eliminate sources of negative externalities and rebuild stocks. We also demonstrate that the closed season does in fact protect the main events of the lobster life cycle. Responsible and efficient management should focus on life cycle studies and reliable monitoring of fishing data.
Vocal Fold Immobility Following Vaccination
Authors: Talmor Guy , Nguyen Brandon , Din-Lovinescu Corina , Paskhover Boris , Kaye Rachel 
Published: October 2020

Objective: Vocal fold immobility (VFI) may severely affect quality of life due to dysphonia and respiratory distress. Many etiologies of this disorder have been evaluated, however the relationship between VFI and vaccination has yet to be explored. The objective of this study was to identify the relationship between VFI and vaccine administration. Methods: The Vaccine Adverse Event Reporting System (VAERS) database was queried for patients exhibiting symptoms of VFI following vaccination. Patient demographics and clinical information including presenting symptoms, time of symptom onset, laterality, outcomes, and adverse events were documented. Results: Twenty-two patients were found to have VFI following vaccination. Of those reported, 13 patients were female (59.1%) and 8 were male (36.4%) with an average age of 48.4 years. Vaccinations for influenza, shingles, pneumococcus, and hepatitis B were reported. A majority of these cases were unilateral in nature (73.3%). Mean lag time from vaccination to symptom onset was 6.3 days (range 0-45 days). Five adverse events were reported, with 4 patients requiring intubation and tracheostomy. Conclusion: Vaccine administration may be associated with VFI and physicians should be cognizant of this potential adverse event. This is a rare complication with less reported cases than other post-vaccination cranial neuropathies. The difficulty in establishing an initial diagnosis and need for specialized evaluation by an otolaryngologist may result in under-reporting of such events. Further research is needed to delineate the exact pathophysiology of this complication and determine whether a causal relationship exists.
Factors Predictive of Outcome in Inferior Turbinate Surgery
Authors: Harju Teemu , Numminen Jura 
Published: October 2020

Objectives: The purpose of the study was to examine the various preoperative predictive factors of inferior turbinate surgery and to find possible factors that predict an optimal subjective response using 3 common surgical techniques—radiofrequency ablation (RFA), diode laser, and microdebrider-assisted inferior turbinoplasty (MAIT)—in a randomized, prospective study with a 1-year follow-up. Methods: The patients filled a visual analogue scale (VAS) questionnaire regarding the severity of nasal obstruction prior to and 1 year after surgery. A VAS score improvement of 3 points or more was chosen as an optimal subjective response. Univariate and multivariate regressions were used to evaluate the effect of the predictive factors. In total, 80 patients attended a 1-year control visit. Results: In the multivariate analysis, patients without anterior septal deviation had a statistically significantly higher odds ratio of a satisfactory subjective response compared to patients with anterior septal deviation (5.6; 95% CI: 1.4-23.1; P = .02). Patients treated with RFA had a statistically significantly higher odds ratio of an optimal subjective response compared to patients treated with MAIT (9.0; 95% CI: 1.5-54.2; P = .02). Conclusions: Anterior septal deviation seems to decrease the likelihood of an optimal subjective response to inferior turbinate surgery, which supports the consideration of concomitant septoplasty at least in clear cases to optimize the subjective response. Radiofrequency ablation had a significantly higher likelihood of an optimal subjective response compared to MAIT. Further investigations regarding the findings are needed.
Effect of Disclosure on Receiving Spousal Support in Korean Immigrants With Type 2 Diabetes
Authors: Choi Sarah , Toyama Joy , Brecht Mary-Lynn 
Published: October 2020

Purpose The purpose of this study was to investigate factors associated with receiving diabetes spousal support in a sample of Korean immigrants with type 2 diabetes (T2DM) and to test whether disclosure (culture-specific factor) is a significant predictor of spousal support receipt in this group. Methods A cross-sectional survey was conducted with a convenience sample of 136 US community-dwelling Korean immigrants with T2DM ages 46 to 89 years old. Potential predictors were sociodemographic factors (age, gender, education, years in US), personal characteristics (quality of marriage and depression), diabetes severity (duration of diabetes, A1C, insulin use), diabetes self-management, diabetes worries/concerns (psychological factor), and disclosure of worries (cultural factor). The study used validated survey instruments including Diabetes Care Profile for spousal support received, Summary of Diabetes Self-Care Activities, Problem Area in Diabetes, and Diabetes Distress Disclosure Index. Descriptive statistics, correlations, and hierarchical multivariable linear regression models were conducted. Results Six predictors (education level, years spent in the US, glucose control status, diabetes self-management level, diabetes worries/concerns, and disclosure of diabetes worries) were significantly related to receiving spousal support. At least some college education, less time in the US, better glucose control (lower A1C), poor self-management, more diabetes worries, and more disclosure of diabetes worries/distress were associated with receiving higher level of spousal support. Disclosure was the strongest predictor. Conclusion Receiving spousal support for diabetes self-management among Korean immigrants is influenced by 6 factors identified in this study. Disclosing diabetes worries (cultural factor) is most impactful.
An Examination of Gender Differences in the National Diabetes Prevention Program’s Lifestyle Change Program
Authors: Jackson Matt C. , Dai Shifan , Skeete Renée A. , Owens-Gary Michelle , Cannon Michael J. , Smith Bryce D. , Jabrah Rajai , Masalovich Svetlana E. , Soler Robin E. 
Published: October 2020

Purpose The purpose of the study was to examine how gender was related to enrollment and number of sessions attended in the National Diabetes Prevention Program’s Lifestyle Change Program (DPP LCP). Methods To better understand program uptake, a population of those who would be eligible for the LCP was compared to those who enrolled. Estimates of those eligible were computed using data from the National Health and Nutrition Examination Survey, whereas enrollment and sessions attended were computed using data from the Centers for Disease Control and Prevention’s Diabetes Prevention Recognition Program. Results Results revealed that although similar numbers of males and females were eligible for the program, only 39 321 males versus 121 007 females had enrolled in the National DPP LCP by the end of 2017 (odds ratio = 3.20; 95% CI, 3.17-3.24). The gender differences persisted even when stratifying by age or race/ethnicity. In contrast, no significant gender differences were found between the average number of sessions attended for males (14.0) and females (13.8). Discussion Results of the study can help inform efforts to market and tailor programs to appeal more directly to men and other groups that are underrepresented in the National DPP LCP.
“It takes a village to get through cancer,” A photo-elicitation study exploring lived experiences of community cancer survivors
Authors: Polo Katie M , Ingram Gabrielle , Mather Tamzyn , Ragle Amy , Scholl Nicole , Welch Taylor 
Published: October 2020

Introduction Cancer survivors continue to experience complications that last months to years following diagnosis and treatment, which can impact daily life. The purpose of this study was to explore the lived experiences of adult community cancer survivors related to daily activities and occupational performance. Method Phenomenology study design and photo-elicitation were utilized to blend photos and words. Thirteen adult survivors were recruited from a community-based cancer support organization. Open coding, axial and in vivo coding, and constant comparative analysis were utilized to analyze data and achieve conceptual saturation. Results Participants experienced a shift in cultural context, wherein they took on a new context defined by their cancer survivorship. This context shift is dynamic and interconnected to the sub-themes of distress, changes in perspectives, and side effects that all ultimately influence occupational performance and engagement. The cultural context shift supported participants’ ability to engage in new, meaningful occupations, supported their new identity, and ultimately created a sense of support and belonging. Conclusion Occupational therapists have a unique position to assist with the adjustment process during this cultural context shift and provide interventions in cancer support communities to assure cancer survivors’ continued performance and engagement in meaningful activities.
Naringenin Sensitizes Resistant C6 Glioma Cells with a Repressive Impact on the Migrating Ability
Authors: J Jayalakshmi , Vanisree Arambakkam Janardhanam 
Published: October 2020

Background: Glioma, the most common form of a malignant brain tumour is characterised by a poor prognosis, which is attributable to its resistance against current therapeutic approaches. Temozolomide (TMZ), a DNA alkylating agent, is the first-line drug for glioma treatment. Long-term treatment using TMZ was reported to culminate in the development of resistance with overexpression of multidrug resistance 1 gene coded protein P-glycoprotein, which in turn releases the drugs from the tumour cells. Purpose: Thus, to circumvent such resistance issues, the current study attempted to explore the effect of naringenin (a flavanone) with proven antiglial tumour potential, in mitigating the features of TMZ resistance. Methods: Colony-forming assay, invasion assay and scratch wound assay were performed among the groups, namely tumour control (C6), vehicle control (V), naringenin (NGEN)-treated, drug-resistant tumour cells (C6R), and drug resistance cells added with NGEN (C6R+NGEN), to examine the impact of NGEN on migration and invasion. The effect of NGEN on filopodia length and density during cell migration was also studied in addition to the matrix metalloproteinases (MMP-2 and MMP-9) and p-ERK levels. Results and Conclusion: NGEN and C6R+NGEN groups had shown significant reduction ( P < .01) in length and density of filopodia, colony formation, invasion and wound healing. Further, NGEN could also modify the assessed protein levels ( P < .001), which were involved in migration and invasion in sensitive and resistant cells. Our study had provided the first evidence on NGEN-induced enhanced sensitivity against TMZ resistance with profound influence as an antimigratory and anti-invasive agent.
Stakeholder Experiences With Gender Identity Data Capture in Electronic Health Records: Implementation Effectiveness and a Visibility Paradox
Authors: Thompson Hale M. 
Published: October 2020

Background Advocates have endorsed transgender visibility via gender identity (GI) data capture with the advent of the Affordable Care Act and electronic health record (EHR) requirements. Visibility in data in order to enumerate a population contrasts with ways in which other LGBT and public health scholars have deployed these concepts. Aims The article aims to assess the effectiveness of GI data capture in EHRs and implications for trans health care quality improvements and research. Method Semistructured interviews were conducted with 27 stakeholders from prominent gender-affirming care providers across the United States. A range of informants shared their experiences with GI data capture. Interviews were coded, themes were identified, and the extended case method was used to contextualize data in relation to key concepts. Results Data capture is effective for increasing patient counts and making quality improvements but limited in terms of enhancing gender-affirming care depending on provider size, type, and competencies. Many challenges were highlighted regarding use of GI data for research, sharing GI data across systems, as well the ways data capture erases the dynamism of GI. These issues create conditions for limited kinds of disclosure, capture of particular categories, and care and treatment barriers. Discussion Stakeholders exposed a visibility paradox emerging from GI data capture. While data fields are created to increase the visibility of trans persons in medical settings and in health research, they work to increase the visibility of some while reducing the visibility of other gender diverse persons, including trans, nonbinary, and cisgender. Conclusion New approaches are needed to explore implications of GI data standardization and the logics of health care in the face of gender expansiveness.