bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prevalence and associated factors for Alcohol Use Disorder among tuberculosis patients: A systematic review and meta-analysis study.
Authors: Necho Mogesie , Tsehay Mekonnen , Seid Muhammed , Zenebe Yosef , Belete Asmare , Gelaye Habitam , Muche Amare 
Published: November 2020

Abstract
Background: Alcohol use disorders (AUD) in tuberculosis patients are complicated with poor compliance to anti-tuberculosis treatment and poor tuberculosis treatment outcomes. However, aggregate data concerning this problem is not available. Therefore, this review aimed to fill the above gap by generating an average prevalence of AUD in tuberculosis patients.Method: Our electronic search for original articles was conducted in the databases of Scopus, PubMed, and EMBASE, African Index Medicus, and psych-info. Besides, the reference list of selected articles was looked at manually to have further eligible articles for the prevalence and associated factors of AUD in tuberculosis patients. The random-effects model was employed during the analysis. MS-Excel was used to extract data and stata-11 to determine the average prevalence of AUD among tuberculosis patients. A sub-group analysis and sensitivity analysis were also run. A visual inspection of the funnel plots and an Eggers publication bias plot test were checked for the presence of publication bias.Result: A search of the electronic and manual system resulted in 1970 articles. However, after removing duplicates and unoriginal articles, only 28 articles that studied 30854 tuberculosis patients met the inclusion criteria. The average estimated prevalence of AUD in tuberculosis patients was 30% (95% CI: 24.00, 35.00). This was with a slight heterogeneity (I2 = 57%, p-value < 0.001). The prevalence of AUD in tuberculosis patients was higher in Asia and Europe; 37% than the prevalence in the US and Africa; 24%. Besides, the average prevalence of AUD was 39%, 30%, 30%, and 20% in studies with case-control, cohort, cross-sectional and experimental in design respectively. Also, the prevalence of AUD was higher in studies with the assessment tool not reported (36%) than studies assessed with AUDIT. AUD was also relatively higher in studies with a mean age of ≥ 40 years (42%) than studies with a mean age < 40 years (24%) and mean age not reported (27%). Based on a qualitative review; the male gender, older age, being single, unemployment, low level of education and income from socio-demographic variables, retreatment and treatment failure patients, stigma, and medication non-adherence from clinical variables were among the associated factors for AUD. Conclusion: This review study obtained a high average prevalence of AUD in tuberculosis patients and this varies across continents, design of studies, mean age of the participants, and assessment tool used. This implied the need for early screening and management of AUD in tuberculosis patients.
Favorable outcome of COVID-19 infection in a patient with congenital myasthenic syndrome
Authors: Bonanno Silvia , Maggi Lorenzo 
Published: November 2020

Abstract
Patients affected by neuromuscular disorders (NMDs) are theoretically at higher risk for severe illness from SARS-CoV2 due to respiratory and swallowing muscle weakness 1. Here we describe the first case of congenital myasthenic syndrome (CMS) who was infected with COVID-19 and showed a positive outcome.
Urea-Extracted Sericin is Potentially Better Than Kojic Acid in the Inhibition of Melanogenesis Through Increased ROS Generation
Authors: Cherdchom Sarocha , Sereemasoun Amornpun , Aramwit Pornanong 
Published: November 2020

Abstract
Background: Hyperpigmentation is a skin disorder, which is caused by an excess production of melanin. The reduction in melanin content without causing undesirable effects is required for the treatment of hyperpigmentation. Sericin is increasingly used as a hyperpigmentation treatment because of its antityrosinase activity. However, the various methods of sericin extraction have an effect on the composition and biological properties. The purpose of this study was to investigate the antioxidant and anti-melanogenic properties of sericin using different extraction methods including acid, base, heat, and urea extraction. Methods: The chemical properties of extracted sericin were assessed in terms of amino acid components, thermal behavior, and UV-vis absorption. The inhibitory effects of sericin on melanogenesis were explored by determining the melanin content and cellular tyrosinase activity in B16F10 cells.Results: Sericin from urea extraction provided different properties when compared with the other extraction methods. Our results indicate that urea-extracted sericin reduced the melanin content and cellular tyrosinase activity more effectively than the other extraction methods. Interestingly, the potential anti-melanogenic activity was more effective than kojic acid, a depigmenting agent used to treat hyperpigmentation.Conclusions: Our results present the potential inhibitory effect of urea-extracted sericin on melanogenesis. Our results support the therapeutic potential of urea-extracted sericin in the treatment of hyperpigmentation and its complications.
Ionic contrast across a lipid membrane for Debye length extension: towards an ultimate bioelectronics transducer
Authors: Lee Donggeun , Lee Suho , Yu Eui-Sang , Lee Taikjin , Kim Jae Hun , Song Hyun Seok , Lee Kwan Hyi , Lee Seok , Han Sang-Kook , Choi Myung Chul , Ryu Yong-Sang , Kim Chulki 
Published: November 2020

Abstract
Despite technological advances in biomolecule detections, evaluation of molecular interactions via potentiometric devices under ion-enriched solutions has remained a long-standing problem. To avoid severe performance degradation of bioelectronics by ionic screening effects, we cover probe surfaces of field effect transistors (FETs) with a single film of the supported lipid bilayer (SLB), and realize outstanding potentiometric signals from receptor-ligand bindings irrespective of ionic strength of bulky solutions by placing an ion-free water layer underneath the SLB. High-energy X-ray reflectometry together with the circuit analysis discovered biochemical findings that effective electrical signals dominantly originated from the sub-nanoscale conformational change of lipids in the course of receptor-ligand bindings. Beyond thorough analysis on the underlying mechanism at the molecular level, the proposed SLB-FET platform ensures the world-record level of sensitivity in molecular detection with excellent reproducibility regardless of molecular charges and environmental ionic conditions.
Hepatic HuR protects against the pathogenesis of non-alcoholic fatty liver disease
Authors: Tian Mi , Li Xinyun , Li Jingyuan , Yang Jianmin , Zhang Cheng , Zhang Wencheng 
Published: November 2020

Abstract
Background: Liver plays an important role in lipid and glucose metabolism. Human antigen R (HuR) as an RNA regulator protein participates in many disease processes. Here, we investigated the specific role of HuR in hepatic steatosis and glucose metabolism.Methods: We investigated the level of HuR in liver from mice fed a normal chow diet (NCD) and high fat diet (HFD). Liver specific HuR knockout (HuRLKO) mice were generated and challenged with an HFD. Lipid levels and glucose metabolism index were examined. Results: HuR was downregulated in livers of HFD-fed mice. HuRLKO mice showed exacerbated HFD-induced hepatic steatosis but improved glucose tolerance as compared with controls. Consistently, HuR inhibited lipid accumulation in hepatocytes. Mechanically, HuR could bind to the mRNA of phosphatase and tensin homology deleted on chromosome 10 (PTEN), thus increasing their stability and translation. Finally, PTEN over-expression alleviated HFD-induced hepatic steatosis in HuRLKO mice.Conclusion: HuR modulates lipid and glucose metabolism through regulating PTEN expression.
Electronic Health Record-Based Genome-Wide Meta-Analysis and Mendelian Randomization Identify Metabolic and Phenotypic Consequences of Non-Alcoholic Fatty Liver Disease
Authors: Ghodsian Nooshin , Abner Erik , Gobeil Émilie , Taba Nele , St-Amand Alexis , Perrot Nicolas , Couture Christian , Mitchell Patricia , Bossé Yohan , Mathieu Patrick , Vohl Marie-Claude , Thériault Sébastien , Tchernof André , Esko Tõnu , Arsenault Benoit 
Published: November 2020

Abstract
Non-alcoholic fatty liver disease (NAFLD) has been associated with several blood biomarkers and chronic diseases. Whether these associations underlie causal effects remains to be determined. We aimed at identifying blood metabolites, blood proteins and human diseases that are causally impacted by the presence of NAFLD using Mendelian randomization. We created a NAFLD genetic instrument from NAFLD loci (MTARC1, GCKR, LPL, TRIB1, LMO3, FTO, TM6SF2, APOE and PNPLA3) identified in a new electronic health record based-GWAS meta-analysis (6715 cases and 682,748 controls). We found a potentially causal effect of NAFLD on tyrosine metabolism as well as on blood levels of eight proteins that could potentially represent new early biomarkers of NAFLD. Using results from the UK Biobank, FinnGen and the COVID-19 Host Genetics Initiative, we found that NAFLD was not causally associated with diseases outside the spectrum of liver diseases, suggesting that the resolution of NAFLD might not prevent other diseases.
Efficient ethylene purification by a robust ethane-trapping porous organic cage
Authors: Su Kongzhao , Wang Wenjing , Du Shunfu , Ji Chunqing , Yuan Daqiang 
Published: November 2020

Abstract
The removal of ethane (C2H6) from its analogous ethylene (C2H4) is of paramount importance in the petrochemical industry, but highly challenging due to their similar physicochemical properties. It is still remaining unexploited to utilize emerging porous organic cage (POC) materials for C2H4/C2H6 separation. Herein, we report the benchmark example of a truncated octahedral calix[4]resorcinarene-based POC adsorbent (CPOC-301), preferring to adsorb C2H6 than C2H4, and thus can be used as a robust absorbent to directly separate high-purity C2H4 from the C2H4/C2H6 mixture. Molecular modelling studies suggest the exceptional C2H6 selectivity is due to the suitable resorcin[4]arene cavities in CPOC-301, which form more multiple C–H···π hydrogen bonds with C2H6 than with C2H4 guests. This work provides a fresh avenue to utilize POC materials for highly selective separation of industrially important hydrocarbons.
A novel histone H3K4me1-specific binding protein is required for siRNA accumulation and DNA methylation at a subset of loci targeted by RNA-directed DNA methylation
Authors: Niu Qingfeng , Song Zhe , Tang Kai , Chen Lixian , Ban Ting , Guo Zhongxin , Kim Chanhong , Zhang Heng , Duan Cheng-Guo , Zhang Huiming , Zhu Jian-Kang , Du Jiamu , Lang Zhaobo 
Published: November 2020

Abstract
In plants, RNA-directed DNA methylation (RdDM) is a well-known de novo DNA methylation pathway that involves two plant-specific RNA polymerases, Pol IV and Pol V. In this study, we discovered and characterized a new RdDM factor, RDM15. Through DNA methylome and genome wide siRNA analyses, we show that RDM15 is required for RdDM-dependent DNA methylation and siRNA accumulation at a subset of RdDM target loci. We show that RDM15 contributes to Pol V-dependent downstream siRNA accumulation, and interacts with NRPE3B, a subunit specific to Pol V. We also show that the C-terminal tudor domain of RDM15 specifically recognizes the histone 3 lysine 4 monomethylation (H3K4me1) mark. Structure analysis of RDM15 in complex with the H3K4me1 peptide showed that the RDM15 tudor domain specifically recognizes the monomethyllysine through an aromatic cage and a specific hydrogen bonding network; this chemical feature-based recognition mechanism differs from all previously reported lower methyllysine recognition mechanisms. RDM15 and H3K4me1 have similar genome-wide distribution patterns at RDM15-dependent RdDM target loci, establishing a link between H3K4me1 and RDM15-mediated RdDM in vivo. In summary, we have identified and characterized a histone H3K4me1-specific binding protein as a new RdDM component, and our structural analysis of RDM15 revealed a new type of chemical feature-based lower methyllysine recognition mechanism.
Immunogenicity and Safety of Concomitant Administration of 23-valent Pneumococcal Polysaccharide Vaccine and Trivalent Inactivated Influenza Vaccine in Adults Aged 50-70 Years
Authors: Liu Yuanbao , Hu Ran , Sun Xiang , Zhang Min , Tang Fenyang , Zhang Lei , Liu Xiaoqin , Shi Xuanwen , Ma Fubao , Luo Linyun , Wang Zhiguo , Chen Haiping 
Published: November 2020

Abstract
Background To explore the serological protective effect and to evaluate the safety after combined immunization with 23-valent pneumococcal polysaccharide vaccine and trivalent inactivated influenza vaccine in adults aged 50-70 years.Methods This trial was designed as a single-center, randomized, single-blind, and positive parallel control vaccination study. A total of 1,065 people aged 50-70 years old were observed in Zhenjiang city, Jiangsu Province. Three groups were established, namely, the combined vaccine group (366 people), the pneumonia vaccine group (363 people) and the influenza vaccine group (364 people). The subjects were randomly assigned to each group.Results A total of 1065 subjects were recruited. The noninferiority criterion of GMC ratios was met for all 7 pneumococcal serotypes after concomitant administration when compared with the administration of PPV23 alone. After concomitant administration, the noninferiority criterion of seroconversion rates was met for all inﬂuenza subtypes except the inﬂuenza H3 virus. The overall incidence of systemic adverse events was 6.67% (71/1065). The most frequently reported systemic adverse events were abnormal body temperature, muscle aches, nausea, and diarrhea. No statistically significant difference in systemic adverse events was found among the three groups.Conclusion The local and systemic response rates in the combination group, pneumonia group and influenza group were similar to those of many similar vaccines, suggesting that the vaccine has good clinical safety. Serological findings from the combination vaccine suggest that it should be encouraged in the future to increase vaccination rates for influenza and pneumonia.Trial Registration: ClinicalTrials.gov NCT02062281 February 13, 2014; Retrospectively registered.
Soil-transmitted Helminthiasis, Intestinal Protozoa and Clonorchiosis Infections in Southeast China: A Cross-sectional Study
Authors: Feng Yan , Yu Kegen , Chen Hualiang , Zhang Xuan , Lu Qiaoyi , Wang Xiaoxiao , Zhang Xueying , Yao Linong , Ruan Wei 
Published: November 2020

Abstract
Background: The large scale epidemiology of parasitic diseases in Zhejiang Province has not been investigated since the second national survey.Methods: We investigated the distribution of three types of parasitic diseases (i.g. soil-transmitted helminthiasis (STH), intestinal protozoa, and clonorchiosis) in Zhejiang province from late 2014 to 2015. Kato-Katz technique was used for STH and Clonorchis. Sinensis detection, transparent adhesive paper anal swab was used for pinworm detection, and iodine smear was for protozoa detection. Hookworm positive samples need to be cultured for identification of Ancylostmoma duodenale or Necator americanus. Results: 23,552 participants were included in this study (19,935 from rural area and 3,617 from urban area). The overall prevalence of any intestinal helminth infection was 1.800%. Seven species of helminth were found: A.duodenale, N.americanus, Trichuris trichiura, Ascaris lumbricoides, C. sinensis, Fasciolopsis buski and pinworm. The prevalence of STH infection was 1.711% (1.941% in rural area, 0.442% in urban area). Hookworm was the most prevalent infection (1.584%). Only 2.79% children were found for pinworm infection in rural area. 0.396% of the rural participants were infected protozoa, and Endolimax nana was the most prevalent (0.231%). C. Sinensis infected only one man. 89.135% of participants had good personal hygiene habits. The awareness of C. Sinensis was 24.467% and 45.963% in rural and in urban area, respectively. Conclusions: Comparing to the second national survey, the prevalence of STH and protozoa infections declined greatly and C. Sinensis infection was still rare in Zhejiang Province. However, hookworm, especially N.americanus was still a parasitic threat to population health. More health education about fertilization and farming habits should be provided in rural areas. The awareness of hookworm and C. Sinensis should be reinforced in both rural and urban areas.
Retrospective Analysis of the Influence of Closure of Mesenteric Defects on&nbsp;Complications During Laparoscopic Radical Resection of Right Colon Cancer
Authors: Du Zhouyuan , Wang Dianshi , Li Yuan , Hu Peng , Wu Chuanqing , Tao Kaixiong 
Published: November 2020

Abstract
Background: The influence of closure of mesenteric defects during laparoscopic radical resection of right colon cancer is still controversial.Methods: A total of 66 patients who were received laparoscopic radical resection of right colon cancer in our hospital were enrolled. The statistics of complications, included Postoperative Length of Stay, Exhaust time after surgery, Defecation time after surgery, Intraoperative blood loss, Length of incision, Length of operation, Lung infection, Abdominal infection, Anastomotic bleeding and Anastomotic leakage were examined.Results: The mesenteric defects were closed in 35 patients in Clousure group and not closed in 31 patients in Nonclosure group.The difference between two group included Total complication, Postoperative length of stay, Exhaust time after surgery, Defecation time after surgery, Length of operation, Lung infection, Abdominal infection, Anastomotic bleeding and Anastomotic leakage are not significant (p>0.05).Conclusion: Whether to close the mesenteric defects during laparoscopic radical resection of right colon cancer does not affect the rate of comlications.
Recycled volatiles determine fertility of porphyry deposits in collisional settings
Authors: Xu Bo , Hou Zeng-Qian , Griffin William L. , Lu Yongjun , Belousova Elena , Xu Ji-Feng , O'Reilly Suzanne Y. 
Published: November 2020

Abstract
An intensive study of the geochemical characteristics (including the volatile elements Cl and S) of apatite associated with porphyry deposits was undertaken to address the debate about the crust- or mantle-derivation of their copper and gold and to better understand the controls on the transport of metals in magmatic fluids in post-subduction settings. New geochemical data on apatite reveal parameters to discriminate mineralized porphyry systems across Iran and western China (Tibet and Yunnan), from coeval barren localities across this post-subduction metallogenic belt. Apatites in fertile porphyries have higher Cl and S concentrations (reflecting water-rich crystallization conditions) than those from coeval barren ones. Our new isotopic data also indicate these volatiles are likely derived from pre-enriched sub-continental lithospheric mantle, metasomatized by previous oceanic subduction. This study demonstrates that refertilization of suprasubduction lithospheric mantle during previous collision events is a prerequisite for forming post-subduction fertile porphyries, providing an evidence-based alternative to current ore-enrichment models.
Sara4r: an R graphical user interface (GUI) to estimate watershed surface runoff applying the NRCS – curve number method
Authors: Hernández-Guzmán Rafael , Ruiz-Luna Arturo , Mendoza Eduardo 
Published: November 2020

Abstract
This paper introduces a graphical user interface (GUI) for the R software that allows the rainfall-runoff relationship to be calculated, using the curve number method. This GUI is a raster-tool whose outputs are runoff estimates calculated using land use/land cover and hydrologic soil group maps. The package allows the user to select among three different antecedent moisture conditions and includes modifications about the initial abstraction parameter. We tested this GUI with data derived from two watersheds in Mexico and the outputs were compared with those produced using a well-established GIS tool in a vector environment. The results produced by these two approaches were practically the same. The main advantages of our package are: (1) ‘Sara4r’ is faster than previous vector based tools; (2) it is easy to use, even for people with no previous experience using R; (3) the modular design allows the integration of new routines; and (4) it is free and open source.
Preparation and characterization of activated carbon developed from cotton cloth residue activated with phosphoric acid: adsorption of clofibric acid
Authors: Boudrahem N. , Aissani-Benissad F. , Boudrahem F. , Vial C. , Audonnet F. , Favier L. 
Published: November 2020

Abstract
Cotton cloth waste was used as a precursor to prepare activated carbon (ACCs) chemically activated with phosphoric acid. Adsorption behavior of prepared ACCs was correlated with physicochemical proprieties. The pore volume and BET surface of ACCs were determined by nitrogen adsorption isotherms and scanning electron microscopy was used to observe their surface morphologies. Fourier transform infrared (FTIR) spectroscopy analysis and pH point zero charge (pHPZC) were conducted to determine chemical properties. Under the optimal conditions: 50% impregnation ratio and thermal treatment under N2 flow at 600 °C during 60 min, the activated carbon prepared exhibits a high surface area 1,150 m2/g, 0.501 cm3/g micropore volume and an excellent adsorption performance. The adsorbed amount of clofibric acid is found to be 9.98 and 83 mg/g at, respectively, initial CA concentration of 10 and 100 mg/L at pH 3.0 and 20 °C. Diffusion and chemisorption are the steps controlling the adsorption of CA onto ACC 50% and the equilibrium data were well described by Freundlich isotherm.
Conformational Dynamics Analysis of MEK1 Using Hydrogen/Deuterium Exchange Mass Spectrometry
Authors: Yun Min Woo , Kim Kiae , Park Ji Young , Chung Ka Young 
Published: November 2020

Background:
Activation of mitogen-activated protein kinases (MAPKs) is regulated by a phosphorylation cascade comprising three kinases, MAPK kinase kinase (MAP3K), MAPK kinase (MAP2K), and MAPK. MAP2K1 and
MAPK2K2, also known as MEK1 and MEK2, activate ERK1 and ERK2. The structure of the MAPK signaling cascade has
been studied, but high-resolution structural studies of MAP2Ks have often focused on kinase domains or docking sites, but
not on full-length proteins.

Objective:
To understand the conformational dynamics of MEK1.

Methods:
Full-length MEK1 was purified from Escherichia coli (BL21), and its conformational dynamics were analyzed
using hydrogen/deuterium exchange mass spectrometry (HDX-MS). The effects of ATP binding were examined by coincubating MEK1 and adenylyl-imidodiphosphate (AMP-PNP), a non-hydrolysable ATP analog.

Results:
MEK1 exhibited mixed EX1/EX2 HDX kinetics within the N-terminal tail through β1, αI, and the C-terminal helix. AMP-PNP binding was found to reduce conformational dynamics within the glycine-rich loop and regions near the DFG
motif, along with the activation lip.

Conclusion:
We report for the first time that MEK1 has regions that slowly change its folded and unfolded states (mixed
EX1/EX2 kinetics) and also report the conformational effects of ATP-binding to MEK1.