bluuebunny/crossref_metadata_2025_split huggingface dataset:
A cross-sectional comparison of breastfeeding knowledge, attitudes, and perceived partners’ support among expectant couples in Mekelle Ethiopia
Authors: Gebremariam kidane , Zelenko Oksana , Mulugeta Afework , Gallegos Danielle 
Published: August 2020

Abstract
Background: Breastfeeding is considered the biological norm and essential to reduce infant morbidity and mortality. Mothers are responsible for breastfeeding but the support of others, including their partners is an influential determinant. The aim of this study was to compare antenatal breastfeeding knowledge, attitudes, and perceived breastfeeding support of expectant couples in Mekelle, Ethiopia. Methods: As part of a mHealth intervention trial , 128 couples in their third trimester from three selected health centres in Mekelle city were recruited to participate. Couples who each had a personal mobile phone, read and spoke Tigrigna, and lived together were included. Baseline data on breastfeeding knowledge, attitudes, and perceived support (breastfeeding savvy, help, appreciation, presence and responsiveness) were collected using previously validated tools through interview by health workers. Result: Compared to mothers, male partners were more likely to indicate their intention to provide breastfeeding appreciation (p=0.02), breastfeeding presence (p=0.002), and breastfeeding responsiveness (p=0.04). Mothers’ prenatal perceptions of their partners’ intended breastfeeding support was lower than fathers’ perceptions to support their partners. Multiparous mothers had more positive perceptions regarding their partners’ breastfeeding savvy (p=0.03), and breastfeeding help (p=0.02) compared to primiparous mothers.Conclusion: Fathers in Ethiopia are potentially strong supporters of breastfeeding practice. Future breastfeeding interventions should promote the involvement of fathers in breastfeeding and encourage mothers to increase their partners’ involvement in breastfeeding.
Molecular-based cross-species evaluation of bovine coronavirus infection in cattle, sheep and goats in Ghana.
Authors: Burimuah Vitus , Sylverken Augustina , Owusu Michael , El-Duah Philip , Yeboah Richmond , Lamptey Jones , Frimpong Yaw Oppong , Agbenyega Olivia , Folitse Raphael , Emikpe Ben , Tasiame William , Owiredu Eddie-Williams , Oppong Samuel , Antwi Christopher , Adu-Sarkodie Yaw , Drosten Christian 
Published: August 2020

Abstract
Background: Apart from the huge worldwide economic losses often occasioned by bovine coronavirus (BCoV) to the livestock industry particularly cattle, continuous surveillance of the virus in cattle and small ruminants is essential in monitoring variations in the virus that could enhance host switching. In this study, we collected rectal swabs from a total of 1,498 cattle, sheep and goats. BCoV detection was based on reverse transcriptase polymerase chain reaction. Sanger sequencing of the partial RNA-dependent RNA polymerase (RdRp) region for postive samples were done and nucleotide sequences were compared with homologous sequences from the GenBank.Results: The study reports a BCoV prevalence of 0.3% consisting of 4 positive cases; 3 goats and 1 cattle. Less than 10% of all the animals sampled showed clinical signs such as diarrhea and respiratory distress except for high temperature which occurred in > 1000 of the animals. However, none of the 4 BCoV positive animals manifested any clinical signs of the infection at the time of sample collection. Bayesian majority-rule cladogram comparing partial and full length BCoV RdRp genes obtained in the study to data from the GenBank revealed that the sequences obtained from this study formed one large monophyletic group with those from different species and countries. The goat sequences were similar to each other and clustered within the same clade. No major variations were thus observed with our isolates and those from elsewhere.Conclusion: Given that Ghana predominantly practice the extensive and semi-intensive systems of animal rearing, our study highlights the potential for spillover of BCoV to small ruminants in settings with mixed husbandry and limited separation between species.
Impact of Patron Saint Festivities on Cholera in Three Communes in Haiti
Authors: Moise Kenny , Achille Aude Mélody , Batumbo Doudou , Bourdeau Bertiny , Rebaudet Stanislas , Lerebours Gérald , Henrys Jean Hugues , Raccurt Christian 
Published: August 2020

Abstract
Introduction Religious pilgrimages are among the anthropogenic factors known to be associated with the transmission of diarrheal diseases, such as cholera. This ecological study aimed to describe the evolution of cholera and assess the relationship between the implementation of the ‘coup de poing’ strategy during the patron saint festivities and the incidence of cholera in the three communes of Cabaret, Carrefour, and Croix-des-Bouquets in Haiti in 2017. Materials/Methods An epidemiological curve was produced to illustrate the evolution of cholera at the communal level. Generalized linear models assuming a Poisson distribution were used to weight the annual cholera incidence of communal sections against variables such as the number of patronal festivities, population density and annual precipitation rates. The number of cases in the week of the festivity as well as one and two weeks later was weighted against patronal festivities and weekly precipitation rates. Results In total, 3 633 suspected cholera cases were continuously reported in three communes in Haiti (Cabaret, Carrefour, Croix-des-bouquets) during the 52-epidemiological week period in 2017. After controlling for rainfall and population density, the implementation of the ‘coup de poing’ strategy during the patron saint festivities was associated with a significant reduction in cholera incidence of 57.23% [PR=0.4277 (97.5% CI: 0.2798-0.6193), p=0.0000244]. The implementation of the strategy was associated with a reduction in cholera incidence of 25.41% one week following patronal festivities. Conclusion This study showed a continuous presence of cholera in three communes in Haiti in 2017 and an association between the implementation of the ‘coup de poing’ strategy during patronal festivities and a reduction in cholera incidence. The findings imply that the multi-partner ‘coup de poing’ strategy may have contributed to the reduced cholera incidence following patron saint festivities and in Ouest department in Haiti 2017.
Improvement of Parent’s Awareness, Knowledge, Perception, and Acceptability of HPV Vaccination After a Structured-educational Intervention
Authors: Sitaresmi Mei Neni , Rozanti Nisrina Maulida , Besty Lamria , Wahab Abdul 
Published: August 2020

Abstract
Background: Regardless of the disease burden of HumanPapilloma Virus (HPV), the vaccine has not been included in the Indonesia National Immunization Program. Since 2017 there was a demonstration program of the HPV vaccination in Yogyakarta Province. This vaccine was given free to female primary school students in the 5 th and 6 th grades (11-13 years old). This study aimed to assess whether a structured-educational intervention focus on HPV increases the parental awareness, knowledge, and perceptions toward HPV and the vaccine acceptability. Methods: We conducted a pre-post structured-educational intervention study from July to August 2017 before the implementation of the HPV vaccination demonstration program, in Kulon Progo District, Yogyakarta Province, Indonesia. Parents of female primary school students grades 5 th and 6 th were selected using a school-based proportional random sampling. A pediatric resident provided a structured-educational intervention, which consists of the burden and risk of HPV disease, as well as the benefit and safety of the vaccine. Parents were required to complete validated self-administered questionnaires before and after the structured-educational intervention. Results: A total of 506 parents participated. Before receiving the structured-educational intervention, parents' awareness of HPV infection and the vaccines were low. Only 49.2% of parents had heard HPV infection, and 48.8% had heard about the vaccine. After the structured-educational intervention, there were significant improvements in parent’s awareness, knowledge, and perceptions of HPV infection, cervical cancer, and HPV vaccination (all p <0.001). HPV vaccine's acceptability increased from 74.3% to 87.4% ( p <0.001). There was a significant correlation between increasing HPV vaccine acceptability with the improvement of awareness, knowledge, and perception toward HPV infection, cervical cancer and HPV vaccination (r=0.32 to 0.53, p <0.001). After the structured-educational intervention, better knowledge and positive perceptions of HPV vaccination were predictive of HPV vaccine's acceptability with OR 1.90 (95%CI:1.40-2.57) and OR 1.31(95%CI:1.05-1.63), respectively. Conclusions: A structured-educational intervention is effective in improving parental awareness, knowledge, and perceptions toward HPV as well as the acceptability of the vaccine. The structured-educational intervention designed for parents have important implications for improving vaccine acceptability.
Evaluation of the relationship among dental fear, scaling root planning and periodontal status using periodontitis stages： a retrospective study
Authors: Liu Yeungyeung , Zhang Caimei , Wu Jingyi , Yu Huimin , Xu Duoling , Xie Chengjie 
Published: August 2020

Abstract
Background: The present study used the new classification of periodontitis and validated questionnaires to assess the relationship among dental fear, scaling and root planning (SRP) pain and periodontal status for clinical evaluation.

Methods: One hundred and twenty periodontitis patients were enrolled for retrospective analysis and staging according to the new classification of periodontitis. All patients included in this study from July 2018 to January 2020 were divided into periodontitis stages. Scaling and root planning (SRP) was performed and Visual analogue scale (VAS) was determined for every patient immediately after SRP. Application of questionnaires including Corah’s Dental Anxiety Scales (DAS), Dental Fear Survey (DFS), and the short-form Dental Anxiety Inventory (S-DAI) were implemented from the first attendance and consequent visits after 6 months. All patient demographic data were collected including age, gender, marital status and education level. The scores of each dental fear scale and combination scales were also recorded. Patients were further subdivided into two categories based on DAS scores (low dental fear group: DAS<13; high dental fear group: DAS≥13). Statistical analysis was performed using t-test, chi-square, pearson and spearman correlative analysis to evaluate the relationship and differences among dental fear, SRP pain and periodontal status.

Results: Compared to pre-SRP treatment, dental fear level of DFS and combination scales were significantly decreased in the post-treatment period for all the periodontitis stages. There was no statistically significant difference between the pre-treatment and post-treatment periods on S-DAI and DAS in stage I and II; meanwhile, there were statistically significant differences between pre-treatment and post-treatment periods on S-DAI and DAS in stage III and IV. The correlation among periodontitis stages, VAS and dental fear level assessed via DAS, DFS and S-DAI, was statistically significant. In the categories divided according to DAS scale, the proportion of high periodontitis stages was significantly increased in high dental fear group (DAS≥13).

Conclusion: SRP can reduce dental fear level in all periodontitis stages, especially in stage III and IV. Correlations exists among periodontal status, dental fear and SRP pain. High dental fear is associated with poor periodontal status.
Vaccination concerns, beliefs and practices among Ukrainian migrants in Poland: A qualitative study
Authors: Ganczak Maria , Bielecki Klaudia , Drozd-Dąbrowska Marzena , Topczewska Katarzyna , Biesiada Daniel , Molas-Biesiada Agnieszka , Dubiel Paulina , Gorman Dermot 
Published: August 2020

Abstract
Objectives

Ukrainians numbering approximately 1.2 million are the largest migrant group in Poland. Data on vaccination coverage among migrants are not collected in EU, including Poland. Therefore, this qualitative study aimed to identify vaccination practices in this migrant group, to explore facilitators and barriers to vaccination and related access to Polish healthcare services.

Methods

In September 2019, a qualitative study of Ukrainian migrants (UMs) living in Szczecin, Poland, and recruited through a snowball sampling method, was conducted. Using a semi-structured topic guide, four focus groups were held with 22 UMs aged 18-45. Participants were asked about their attitudes towards vaccination in general with comparison between services in Poland and Ukraine. Following transcription and translation, a thematic analysis was conducted.

Results

Respondents were distrustful of Ukrainian vaccination policy, medical personnel and individual vaccines, however, they often returned to Ukraine for dental and gynaecological appoint-ments. While critical with regards to registering with Polish GPs practices, UMs were confident in health professionals, as well as vaccine delivery. Vaccines were perceived as safer and of better quality than in Ukraine. Difficulties in translating vaccination records were rarely reported, verbal communi-cation was not problematic due to language similarities. All UM parents reported vaccinating their children according to the Polish schedule. However, a significant number of adult UMs have not completed mandatory vaccinations, although they may have obtained false immunization certificates; according to UMs those can be obtained by bribing. Participants reported lower acceptance of the influenza vaccine, mainly due to perceptions around its importance; none had been vaccinated against influenza. None of UMs had heard of the HPV vaccine. UMs experienced challenges in accessing credible online vaccination information in Ukrainian, no official local health authority vaccination material existed either, except for information about measles.

Conclusions

This study pinpointed positive UM attitudes and practices regarding child vaccination in the Polish healthcare system and identified issues for improvement, such as adult vaccination. Health communication should be more tailored within UMs information delivery systems to enable migrants to make informed choices about vaccination. Further research is needed to better assess factors affecting vaccine uptake identified in this study.
Systemic inflammation and chronic kidney disease in a patient due to the RNASEH2B defect
Authors: He Tingyan , Xia Yu , Yang Jun 
Published: August 2020

Abstract
Introduction: Aicardi-Goutières (AGS) is a rare immune dysregulated disease due to mutations in TREX1, RNASEH2A, RNASEH2B, RNASEH2C, SAMHD1, ADAR1, or IFIH1. Clinical features include basal ganglia calcifications, white matter abnormalities, and cerebral atrophy. Severe systemic inflammation and chronic kidney disease (CKD) are extremely rare in AGS. Herein, we report a patient presenting with systemic inflammation and CKD to broaden the clinical phenotype spectrum of the RNASEH2B defect. Methods: All testing and molecular genetic analysis were performed after obtaining the informed consent of the parents. Demographic, clinical, and laboratory findings were abstracted from outpatient and inpatient encounters. Cerebral magnetic resonance imaging (MRI), computed tomography (CT) scans, and renal biopsy histopathology reports were reviewed and summarized. Whole exome sequencing (WES) was performed on peripheral blood cells. After exposure to cGAMP in vitro for 24 hours, mRNA expression of twelve IFN-stimulated cytokine genes in PBMCs was assessed. Serum cytokine levels were detected by Milliplex. Results: A 11-year-old girl presented with recurrent aseptic fever, arthritis, chilblains, failure to thrive, mild hearing loss, and neurological manifestations. Laboratory and immunologic findings demonstrated lymphopenia, low complement levels, positive autoantibodies, elevated levels of acute-phase reactants and inflammatory cytokines. Cerebral imaging showed cerebral atrophy, white matter abnormalities, and intracranial calcification. Renal biopsy showed glomerular sclerosis in three of fourteen glomeruli, infiltration of lymphocytes and other mononuclear cells. WES revealed a homozygous and heterozygous mutations in RNASEH2B . Over-expression of IFN-stimulated cytokine genes was observed, including IFI44, IFI27, IFIT1, IFIT2, IFIT3, ISG15, OAS1, and SIGLEC1. Conclusions: To date, only two cases with AGS have been reported to have renal disease. Here, we describe a patient with both homozygous and heterozygous variants in RNASEH2B, presenting with neurological manifestations, persistently systemic autoinflammation, and CKD. CKD has never been reported in patients with AGS due to the RNASEH2B defect .
Electrocardiographic findings of methanol toxicity: A cross-sectional study on 356 cases in Iran
Authors: Nikoo Mohammad Hossein , Arjangzadeh Alireza , Pakfetrat Maryam , Boogar Shahrokh Sadeghi , Mohammadkarimi Vahid , Ostovan Vahid Reza , Khodamoradi Zohre , Roozbeh Jamshid , Khalili Mohammadreza , Shirazi Farnaz Kamali Haghighi , Kouhi Paryia , Heydari Seyyed Taghi 
Published: August 2020

Abstract
Background : Methanol is widely used in industry; however, methanol poisoning is not common. In this regard, a number of outbreaks have been recently reported due to inappropriate processing of alcoholic beverages. Shiraz, a city located in the southern part of Iran, faced one of such outbreaks in 2020 during COVID-19 pandemic. There is no sufficient literature on the electrocardiographic findings in methanol toxicity. This study aimed to address this gap in the literature. Method : A total of 356 cases with methanol toxicity referred to Shiraz University of Medical Science Tertiary Hospitals (Faghihi and Namazi) in March and April, 2020. The clinical findings of blindness and impaired level of consciousness, lab data such as arterial blood gas, electrolytes, and creatinine, and the most common findings from ECGs were collected. Results : The most common ECG findings were J point elevation (68.8%), presence of U wave (59.2%), QTc prolongation (53.2% in males and 28.6% in females), and fragmented QRS (33.7%). An outstanding finding in this study was the presence of myocardial infarction in 5.3% of the cases. This finding, to the best of our knowledge, has only been reported in a few case reports. Brugada pattern (8.1%) and Osborn wave (3.7%) were the other interesting findings. In multivariate analysis, when confounding factors were adjusted, myocardial infarction, atrioventricular conduction disturbances, sinus tachycardia, and the prolonged QTC>500 msecond were four independent factors correlated with methanol toxicity severity measured with arterial blood PH on arterial blood gas measurements, with odds ratios of 12.82, 4.46, 2.32 and 3.15 (P<0.05 for all) , respectively. Conclusion : Electrocardiographic variations during methanol intoxication are remarkable and well-correlated with poisoning severity. Myocardial infarction was an egregious and yet a common concerning finding in this sample, which need to be ruled out in methanol toxicity.
Aspects of cultivar variation in physiological traits related to Cd distribution in rice plants with a short-term stress
Authors: Chiao Wan-Ting , Chen Bo-Ching , Syu Chien-Hui , Juang Kai-Wei 
Published: August 2020

Abstract
Background

Genotypic variations are seen in cadmium (Cd) tolerance and accumulation in rice plants. Cultivars that show low Cd translocation from the root into shoot can be selected to reduce Cd contamination in rice grains. This study aims to clarify the physiological regulation related to Cd absorption by rice plants for screening out the cultivars, which have relatively low Cd accumulation in grains. Eight Taiwan mega cultivars of paddy rice: japonica (TY3, TK9, TNG71, and KH145 cultivars), indica (TCS10 and TCS17 cultivars), and glutinous (TKW1 and TKW3 cultivars), which are qualified with the criteria for rice grain quality by the Council of Agriculture, Taiwan, were used for illustration. An experiment in hydroponics was conducted for the rice seedlings with a treatment of 50 μM CdCl 2 for 7 days.

Results and discussion

After the Cd treatment, the reductions in shoot growth were more significant than those in root growth; however, Cd absorbed in the rice plant was sequestered much more in the root. The malondialdehyde (MDA) was preferentially accumulated in rice root but the hydrogen peroxide (H 2 O 2 ) was increased more significantly in the shoot; the antioxidative enzymes, superoxide dismutase (SOD) and ascorbate peroxidase (APX), were pronounced more in rice shoot.

Conclusions

The rice cultivars preferentially accumulated Cd in the root rather than the shoot with the Cd treatment, which resulted in significant enhancements of MDA and growth reductions in the root. However, H 2 O 2 accumulation was toward the shoot to retard shoot growth suddenly and then the root could keep a gradual growth. Also, the rice cultivars, which preferentially accumulate Cd in the root, would have the regulation tendency of SOD toward the shoot. Due to that SOD is responsible for H 2 O 2 production, H 2 O 2 accumulation would be thus toward the shoot. Moreover, the cultivars, which have a less regulation tendency of APX toward the shoot, would present higher translocation of Cd into the shoot.