bluuebunny/crossref_metadata_2025_split huggingface dataset:
diagnostic utility of procalcitonin in children with infectious complications during chemotherapy-induced neutropenia: single center experience, literature review
Authors: Dinikina Yu. V. , Toshina Yu. K. , Belogurova M. B. 
Published: April 2020

Background. Infectious complications cause significant mortality in children with oncological diseases during chemotherapy-induced neutropenia. The absence of sensitive and specific signs and symptoms of infectious conditions as well as its microbiological identification, leads to inappropriate antibiotic exposure. The use of laboratory biomarkers (procalcitonin (PCT) and C-reactive protein (CRP)) may be helpful for differential diagnostics of inflammatory conditions and for rational antimicrobial therapy.Objective: to assess the current value of PCT as an additional marker for differentiating inflammatory conditions in children with chemotherapy-induced neutropenia.Materials and methods. We presented the analysis of infectious complications in pediatric patients with oncological and onco- / hematological diseases between 2017–2020 (54 patients from 2 mnths – 17 years). PCT and CRP with clinical and instrumental diagnostic data were used for differential diagnosis of fever and development of antimicrobial therapy decision rules. Literature review concerning the discussed theme from 2006–2018 was done.Results. Eighty-five infectious episodes in 36 months were registered, among them 42 in pts with onco- / hematological diseases and 43 – with solid tumors. In the group of bacterial infectious complications mean CRP and PCT values were significantly higher than in group of nonbacterial, moreover the discriminative value was higher for PCT. We revealed the correlation between severity of infectious complications and values of markers of acute-phase reactions. In case of non-severe bacterial complications and other types of infections significant difference was revealed only for PCT mean values.Conclusion. Specificity of PCT concentration in bacterial infections exceeds that of CRP, which confirms the hypothesis of advantages in using PCT as differential marker of inflammatory conditions in children with malignancies.
Tipificación del virus del papiloma humano en mujeres con lesión escamosa intraepitelial de bajo grado
Authors: González-Losa María del Refugio , Rosado-López Iván , Valadez-Gonzalez Nina , Cámara-Mejía Javier , Puerto-Solís Marylin 
Published: April 2020

Objetivo: determinar la prevalencia y los genotipos de los papillomavirus humanos (PVH) en mujeres con lesiones escamosas intraepiteliales de bajo grado (LEIBG).Métodos: noventa y seis mujeres de la clínica de colposcopia fueron incluidas. La presencia del ADN de los PVH en las biopsias fue determinada por ensayo de reacción en cadena de la polimerasa (RCP) con oligonucleótidos para el gen L1. Las muestras fueron coamplificadas con MY09/MY11/HMB01 e iniciadores para betaglobina GH20/PC04. Para tipificar se utilizó hibridación reversa en línea.Resultados: el ADN de PVH fue detectado en 28/96 (29,1%) pacientes, de las cuales 21/28 (75%) tuvieron infección con un solo tipo y 7/28 (25%); infección múltiple. En 29/39 (74,3%) fueron de alto riesgo y 10/39 (25,7%) de bajo riesgo. Encontramos 13 diferentes tipos, 9/13 (69,2%) de ellos de alto riesgo. PVH 58 fue el tipo más frecuente.Conclusiones: La prevalencia de VPH en mujeres con diagnóstico citológico de LEIBG fue menor que la esperada; este resultado sugiere una clasificación inadecuada de los diagnósticos.
Tuberculosis peritoneal en una paciente con ascitis y masa anexial. Reporte de caso
Authors: García Germán , García Andrés José , Goez Elvia Inés , Saldarriaga Clara Inés 
Published: April 2020

Objetivo: presentar el cuadro clínico y de laboratorio de una paciente con tuberculosis peritoneal. La tuberculosis puede afectar diferentes órganos con presentaciones clínicas que simulan otras patologías.1Presentamos el caso de una paciente con ascitis y masa anexial diagnosticada por tomografía y con CA 125 elevado. Se realizó laparotomía exploradora con la impresión diagnóstica de tumor de ovario, encontrando adherencias y siembras peritoneales. La patología informó inflamación granulomatosa crónica compatible con tuberculosis.
OPTIMASI DAN EFISIENSI TEKNIK ISOLASI RNA DAUN DAN AKAR KELAPA SAWIT (Elaeis guineensis Jacq)
Authors: Mukmin Restu Prasetya 
Published: April 2020

Tanaman kelapa sawit memiliki kandungan polisakarida dan polifenol yang tinggi. Kontaminasi polisakarida dan polifenol menyebabkan sulitnya proses isolasi RNA dari jaringan tanaman kelapa sawit. Penelitian ini dilakukan untuk mengoptimasi beberapa protokol isolasi RNA tanaman kelapa sawit yang efektif dan efisien. Penelitian dilaksanakan selama tiga bulan, pada bulan Oktober-Desember 2018 di Laboratorium Biokimia dan Biologi Molekuler, Pusat Penelitian Bioteknologi dan Bioindustri Indonesia (PPBBI), Jalan Taman Kencana No. 1 Bogor 16128. Penelitian dilakukan dengan melaksanakan teknik isolasi RNA menggunakan tiga protokol, yaitu modifikasi Cetyl Trimethyl Ammonium Bromide (CTAB), kit isolasi RNA RNeasy Plant Mini Kit (Qiagen), dan kit isolasi NucleoSpin RNAPlant (Macherey-Nagel). Sampel yang digunakan adalah daun dan akar tanaman kelapa sawit berumur kurang dari tiga bulan dengan bobot 0,1 gram dan 2,5 gram yang disesuaikan untuk tiap protokol. Variabel yang diamati adalah konsentrasi (ng/µl), kemurnian (rasio A260/A280 dan A260/A230), dan pita RNA pada elektroforesis gel agarosa. Hasil penelitian menunjukkan, RNA total hasil isolasi protokol NucleoSpin RNAPlant (Macherey-Nagel) memiliki kualitas paling tinggi. Konsentrasi RNA total daun dan akar kelapa sawit yang didapatkan melalui protokol NucleoSpin RNAPlant (Macherey-Nagel) sebesar 338 ng/µl dan 184,4 ng/µl dengan rasio A260/A280 RNA total daun dan akar kelapa sawit sebesar 2,13 dan 2,18 serta rasio A260/A230 sebesar 2,09 dan 2,20. Hasil elektroforesis gel agarosa menunjukkan integritas yang bagus dari RNA total hasil isolasi RNeasy Plant Mini Kit (Qiagen) dan NucleoSpin RNAPlant (Macherey-Nagel), namun terdapat kontaminasi dan smear pada RNA total hasil isolasi CTAB modifikasi 1 dan 2. Kata kunci: kelapa sawit, isolasi RNA, spektrofotometer, elektroforesis gel agarosa
Content of Prussic Acid and Production of Sorghum Brown Midrib by Adding Urea Fertilizer and Extending Harvesting Time
Authors: Umami Nafiatul , Isnaini Nofi , Suhartanto Bambang 
Published: April 2020

Abstract. The aimed of this study to determine the effect of adding urea fertilizer (0, 50, and 100 kg/ha) and harvesting time (50, 80 and days) on prussic acid content and production of the brown midrib (BMR) sorghum plants. This study design was completely randomized factorial 3 x 3. The variables measured were prussic acid content, fresh yield, the production of dry matter (DM) and organic matter (OM). The results showed that content of prussic acid increased and highly significant with the addition of urea. The fresh yield of BMR sorghum is highest in the fertilization of 50 kg/ha and at the harvesting time 80 days. The highest DM production at fertilization 0 kg/ha and harvesting time 110 day. OM production of the highest is the addition of fertilizer 0 kg/ ha and at the harvesting time on 80 days. From the results of this study concluded that on harvesting time 80 day sorghum BMR have produces optimum and addition of urea to a level of 100 kg/ha did not affect the content of prussic acid, fresh yield, DM and OM.
Effect Education of DASH DIET on Dietary Intake Adherence and Blood Pressure of Hypertension Outpatients in Rumkital Marinir Cilandak
Authors: Uliatiningsih Rita , Fayasari Adhila 
Published: April 2020

Background: Diet DASH (Dietary Approaches To Stop Hypertension) is one of the methods to lowering high blood pressure. Dietary compliance is one of the important things that hypertensive patients need to care of. The hypertensive patients should obey DASH diet in order to avoid further complication. This study aims to discover the effect of DASH diet education on diet adherence and blood preesure re in hypertensive patients in Cilandak Marine Hospital, Jakarta.Method: This study used quasi experimental design with pretest-posttest one group design approaches. There were 75 respondents in this study which were obtained using purposive sampling type. The data were obtained from Cilandak Marine Hospital, Jakarta. The statistical analysis used paired sample t test. Result: The result of the study showed that dietary compliance of DASH diet with blood pressure in hypertensive patients in Cilandak Marine Hospital, Jakarta were categorized 3 parts as non compliants when the score is 31-45 for as many as 32 respondents (42,7%), fair compliants when the score is 46-60 for as many as 39 respondent (52,0%) and 4 respondents (5,3%) are categorized in compliant score 61-75. The analysis of the systole and diastole of the blood pressure before and after the intervention in hypertensive patients showed a significant related correlation (p=0,000) .Conclusion: There is a significant correlation between the dietary compliance of DASH diet with blood pressure in hypertensive patients in Cilandak Marine Hospital, Jakarta.
ASUPAN LEMAK SEBAGAI FAKTOR DOMINAN TERHADAP OBESITAS SENTRAL PADA WANITA DEWASA
Authors: Triyanti Triyanti , Ardila Putri 
Published: April 2020

Obesitas sentral berhubungan dengan penyakit kardiovaskular dan infark mikardium. Keduanya berhubungan erat dengan kematian. Prevalensi obesitas sentral terus meningkat tidak hanya di perkotaan tetapi juga pedesaan. Tujuan dari penelitian ini adalah untuk mengetahui prevalensi obesitas sentral dan beberapa faktor yang terkait di kelomok perempuan dewasa. Penelitian ini melibatkan 120 orang perempuan dewasa berusia 20-59 tahun sebagai sampel penelitian di desa Ketug, Purworejo, Jawa Tengah. Data sosiodemografi dikumpulkan menggunakan kuesioner dan lingkar pinggang diukur langsung oleh peneliti. Berdasarkan ambang batas (≥80 cm), didapatkan prevalensi obesitas sentral sebesar 47,5%. Berdasarkan analisis bivariat terdapat hubungan yang bermakna antara obesitas sentral dengan faktor umur, asupan energi, asupan lemak, asupan karbohidrat, riwayat obesitas sentral orang tua, dan jumlah anak yang dilahirkan. Berdasarkan analisis regresi logistik ganda didapatkan asupan lemak sebagai faktor dominan terhadap obesitas sentral.
EFEK LAMA WAKTU PEMBERIAN EKSTRAK ETANOL SELEDRI (Apium graveolens L.) TERHADAP PENCEGAHAN PENINGKATAN KADAR IL-1β PADA TIKUS MODEL ISCHEMIA-REPERFUSION INJURY
Authors: Afifah Afifah , Muflikhah Khusnul , Ati Viva Ratih Bening 
Published: April 2020

Ischemia-reperfusion injury (IRI) is the condition that disrupted the blood supply to the organ followed by the restoration of blood flow. IRI in the kidneys promotes the inflammatory cascade. Interleukin-1β (IL-1β) belongs to the most powerful pro-inflammatory cytokine that released in the early phase of IRI. Prevention of inflammation is one of the strategies for reducing kidney damage due to IRI. Celery (Apium graveolens L) is a natural resource that reported has anti-inflammatory and antioxidant effects. This study aimed to investigate the effect of duration of the administration ethanol extract of celery on IL-1β level in the IRI rat model. Twenty-five rats male Sprague Dawley, 2-3 months old were divided into 5 groups: Sham operations (SO, n=5), Ischemia-reperfusion (IR, n=5), celery 1000 mg/ kg BW for 7 days before IR (IR7) (IR7, n=5), 14 days before IR (IR14, n=5), 28 days before IR (IR28, n=5). The IL-1β level was assessed using the ELISA. Data were analyzed using One-way ANOVA (p<0.05). The results showed that the mean of IL-1β levels in the IR7 group (5.99±4.28 ng/L) and R14 (4.68±2.64 ng/L) were lower than IR group (8.19±5.36 ng/L), while the R28 group (9.05 ± 4.38 ng/L) was higher than the IR group (8.19±5.36 ng/L). In conclusion, the administration of celery ethanol extract 1000 mg/ kg BW for 7 days and 14 days can prevent the increase of IL-1β level in the IRI rat model.

Ischemia-reperfusion injury (IRI) adalah suatu keadaan yang terjadi ketika suplai darah sebagian atau seluruh organ terganggu diikuti pemulihan aliran darah. IRI pada ginjal memicu terjadinya inflamasi. Interleukin-1β (IL-1β) termasuk sitokin pro-inflamasi paling kuat yang keluar selama fase awal IRI. Pencegahan terhadap inflamasi merupakan strategi untuk menurunkan kerusakan ginjal akibat IRI. Seledri merupakan salah satu bahan alam yang diketahui mempunyai efek antiiflamasi dan antioksidan. Penelitian ini bertujuan mengetahui efek lama waktu pemberian ekstrak etanol seledri terhadap pencegahan peningkatan kadar IL-1β pada tikus model IRI. Sebanyak 25 ekor tikus jantan Sprague Dawley, 2-3 bulan, dikelompokkan menjadi 5, yaitu Sham Operation (SO, n=5), Ischemia-reperfusion (IR, n=5), seledri 1000 mg/kgBB selama 7 hari sebelum IR(IR7, n=5), 14 hari sebelum IR(IR14, n=5), dan 28 hari sebelum IR(IR28, n=5). Kadar IL-1β diukur menggunakan metode ELISA. Data dianalisis dengan One Way ANOVA (p<0,05). Rerata kadar IL-1β pada kelompok IR7 (5,99±4,28 ng/L) dan IR14 (4,68±2,64 ng/L) lebih rendah dibandingkan dengan kelompok IR (8,19±5,36 ng/L), sedangkan kelompok IR28 (9,05±4,38 ng/L) lebih tinggi dibandingkan dengan kelompok IR (8,19±5,36 ng/L). Pemberian ekstrak etanol seledri 1000 mg/kgBB selama 7 hari dan 14 hari dapat mencegah peningkatan kadar IL-1β pada tikus model IRI.
B Cell Metabolism: An Understudied Opportunity to Improve Immune Therapy in Autoimmune Type 1 Diabetes
Authors: Wilson Christopher S. , Moore Daniel J. 
Published: April 2020

Abstract
Although B lymphocytes are a key cell type that drives type 1 diabetes (T1D), therapeutic targeting of these cells has not ameliorated disease, and it has been impossible to eliminate autoantibody production clinically once it begins. This challenge indicates a need for further dissection of the cellular processes responsible for the development and activation of autoreactive B cells in T1D. Review of the literature in T1D and other autoimmune and hematopoietic diseases indicates that cellular metabolism contributes significantly to lymphocyte development and fate. Unfortunately, little is known about the normal metabolism of B cells and even less is known about the metabolism of B cells in T1D other than what can be inferred from other immune processes. Clues derived from the literature suggest B cell metabolism in T1D is altered including potential differences in OXPHOS, glucose metabolism, fatty-acid metabolism, and reactive-oxygen species stress response. Future research should dissect the metabolic processes at play in autoreactive B cells in T1D. Once understood, B cell metabolism will become a promising target to use in conjunction with current clinical therapies in T1D. Additionally, metabolic changes in B cells may serve as a reliable biomarker for predicting the responsiveness of patients to these immune therapies.
Investigation and Analysis of Standardized Training for Residents of General Practitioners of Gansu Province in China
Authors: Cai Hui , Wang Hongjing , He Jin , Zhang Dongzhi , Wu Yue , Wang Peidong 
Published: April 2020

Abstract
Background: China’s standardized training for residents of General Practitioners (GPs) is aimed at providing the postgraduate training for family doctors who will serve the primary health care institutions. The aim of this paper is to investigate the standardized training situation, satisfaction with standardized training, work situation, intention, satisfaction and attitude of GPs who have finished standardized training. Methods: This study was undertaken in 6 training hospitals in Gansu province using a questionnaire with 45 questions. Results: Approximately 275 residents of GPs were enrolled. Finally, 263 residents completed the questionnaire (95.64% response rate), including 133 females (50.57%) and 130 males (49.43%) with an average age of 28 years (standard deviation, 1.93 years; range, 25-36 years). Additionally, 56.65% were single and 43.35% were married. Of all subjects, 92.40% residents had obtained certification of standardized training for residents of GPs and only 39.54% residents were satisfied with monthly income during training. There were 171 oriented rural medical graduates, of whom, only 42.69% expressed the willing to continue working in the primary health care institutions after the serve time (6 years) expired. Around 86.31% of residents of GPs who had finished standardized training got jobs with more than half serving in the primary health care institutions. For medical institutions and sanitary bureau were clear about general medicine policies, only 29.96% subjects registered as GPs. Among the residents in general practice department, 68.42% were engaged in the diagnosis and treatment of common disease and frequently-occurring diseases as well as referral of patients. The percentage of residents who were satisfied with the job and income were 30.40% and 14.98%, respectively. Conclusion: Standardized training for residents of GPs in China is gradually improving. In order to cultivate more GPs and increase their willing to serve in the primary health care institutions, it is necessary to formulate and execute better policy of GPs, publize general medicine and improve the training quality.
Trends in Measles Cases in Bayelsa State, Nigeria: A Five-year Review of Case-based Surveillance Data (2014- 2018).
Authors: AWORABHI NENI , Numbere Tamunowari , Balogun Mohammed Shakir , Usman Aisha , Utulu Rowland , Ebere Nkechi , Omubo Wakiente , Stow Jones , Abba Shehu , Olorukooba Abdulhakim 
Published: April 2020

Abstract
Background Measles is a vaccine preventable, highly transmissible viral infection that affects mostly children under five years. It has been ear marked for elimination and Nigeria adopted the measles elimination strategies of the World Health Organization (WHO) African region to reduce cases and deaths. This study was done to determine trends in measles cases in Bayelsa state, to describe cases in terms of person and place, identify gaps in the case-based surveillance data collection system and identify risk factors for measles infection. Methods We carried out a secondary data analysis of measles case-based surveillance data for the period of January 2014 to December 2018 obtained in Microsoft Excel from the State Ministry of Health. Cases were defined according to WHO standard case definitions. We calculated frequencies, proportions, estimated odds ratios (OR), 95% confidence intervals (CI) and multivariate analysis. Results A total of 449 cases of measles were reported. There were 245(54.6%) males and the most affected age group was 1-4 years with 288(64.1%) cases. Of all cases, 289(9.35%) were confirmed and 70 (48.27%) had received at least one dose of measles vaccine. There was an all-year transmission with increased cases in the 4 th quarter of the year. Yenegoa local government area had the highest number of cases. Timeliness of specimen reaching the laboratory and the proportion of specimens received at the laboratory with results sent to the national level timely was below WHO recommended 80% respectively. Predictors of measles infection were, age less than 5 years (AOR: 0.57, 95% CI: 0.36-0.91) and residing in an urban area (AOR: 1.55, 95% CI:1.02-2.34). Conclusions Measles infection occurred all-year round, with children less than 5 years being more affected. Measles case-based surveillance system showed high levels of case investigation with poor data quality and poor but improving indicators. Being less than 5 years was protective of measles while living in urban areas increased risk for infection. We recommended to the state government to prioritize immunization activities in the urban centers, start campaigns by the 4th quarter and continue to support measles surveillance activities and the federal government to strengthen regional laboratory capacities.
Meta-analysis of the diagnostic performance of circulating microRNAs for pancreatic cancer
Authors: Peng Cheng , Li Zhiqiang , Huang Lihua , Gao Wenzhe , Wang Jiale , Liu Yunfei , Li Xia , Yu Xiao 
Published: April 2020

Abstract
Background: Pancreatic cancer (PC) is characterized by high malignancy and a poor prognosis. The detection of circulating microRNAs (miRNAs) is a liquid biopsy diagnostic approaches. Numerous studies have suggested that some differentially expressed miRNAs may be promising diagnostic markers for PC, but the results have varied among studies. The present study was performed to summarize the diagnostic accuracy of circulating miRNAs, carbohydrate antigen 19-9 (CA19-9), and the combination of miRNAs and CA19-9.Methods: A literature search of online databases including PubMed, EMBASE, Cochrane Library, China National Knowledge Infrastructure (CNKI) and WanFang was conducted. Relative data were extracted from eligible included studies, and a meta-analysis was performed.Results: A total of 46 studies involving 4,326 PC patients and 4,277 non-PC controls were included. The pooled sensitivity (SEN), specificity (SPE) and AUC of the circulating miRNAs for differentiating PC patients from non-PC controls were 0.79 (0.77-0.81), 0.77 (0.75-0.79), and 0.85 (0.81-0.87), respectively. For CA19-9, the SEN, SPE and AUC were 0.78 (0.75-0.80), 0.90 (0.85-0.94) and 0.85 (0.82-0.88), respectively. The combination of miRNAs and CA19-9 greatly improved the SEN, SPE and AUC to 0.84 (0.80-0.87), 0.91 (0.89-0.93) and 0.94 (0.92-0.96), respectively. Moreover, circulating miRNAs also yielded an acceptable diagnostic accuracy for early-stage PC with a SEN of 0.79 (0.76-0.82), a SPE of 0.74 (0.68-0.79) and an AUC of 0.81 (0.77-0.84).Conclusions: Circulating miRNAs exhibited satisfactory diagnostic performance for PC and even early-stage PC. The combination of circulating miRNAs and the traditional marker CA19-9 can further improve the diagnostic accuracy, providing a novel strategy for PC diagnosis.
Reverse reaming distraction for acetabular reconstruction of chronic pelvic discontinuity
Authors: Sun Jing-yang , Ni Ming , Ma Hai-yang , Du Yin-qiao , Shen Jun-min , Chen Ji-ying , Zhou Yong-gang , Zhang Guo-qiang 
Published: April 2020

Abstract
Background: The acetabular distraction technique demonstrates encouraging radiographic and clinical outcomes in treating chronic pelvic discontinuity. The aim of this study is to describe a modified distraction technique and to show our results. Methods: This study identified 12 cases of chronic pelvic discontinuity undergoing primary or revision total hip arthroplasty (THA) with the technique of reverse reaming distraction between July 2015 and November 2018. All 12 patients had a minimum follow-up of 12 months. Radiographs were reviewed to inspect for component loosening. Clinical assessment included the Harris hip score (HHS) and an ambulatory scoring system. Results: At the time of final follow-up, no patient was revised. One patient had up to 1 cm migration of the cup in a horizontal or vertical direction and more than 20° change in the abduction angle but was asymptomatic. In the remaining 11 patients, no migration of the component was detected. Both the HHS and ambulatory score showed improvement in all patients. There were no perioperative complications. No postoperative dislocation occurred. Conclusions: Reverse reaming distraction is a feasible technique in treatment of chronic pelvic discontinuity, with encouraging results at early-term. However, ongoing follow-up is required to determine the long-term prognosis in patients receiving this technique.