bluuebunny/crossref_metadata_2025_split huggingface dataset:
High prevalence of SARS-CoV-2 Infection Among Symptomatic Healthcare Workers in a large university tertiary hospital in São Paulo, Brazil
Authors: Buonafine Carolina Palamin , Paiatto Beatriz Nobre Monteiro , Leal Fabyano , Matos Samantha , Moraes Camila Ohomoto , Guerra Giovanna Guazzelli , Martuchelli Marcus Vinicius Vidal , Oliveira Danielle Leal , Durigon Edison Luiz , Soares Camila Pereira , Candido Erika Donizette , Telezynski Bruna Larotonda , Safadi Marco Aurelio , Almeida Flavia Jacqueline 
Published: July 2020

Abstract
Background: Latin America became the epicenter of the COVID-19 pandemic in May 2020, mostly driven by Brazil's situation. Healthcare workers are at increased risk of SARS-CoV-2 infection, experiencing a significant burden from COVID-19. Identifying and understanding the clinical characteristics and risk factors associated with infection are of paramount importance to inform screening strategies and infection control practices in this scenario. The aims of this study were to investigate the prevalence and clinical characteristics of healthcare workers with COVID-19 symptoms. Methods: Between March 21st and May 22nd, 2020 a cross-sectional study was performed in a tertiary university hospital in São Paulo. Prevalence of SARS-CoV-2 infection among health care workers with COVID-19 symptoms was determined by RT-PCR testing on nasopharyngeal and oropharyngeal samples. Participants were asked to complete an electronic structured questionnaire including clinical and demographic data. Results: Overall, 125 (42.37%) of 295 symptomatic healthcare workers tested positive for SARS-CoV-2. Over the 10-week study period, positivity rates varied from 22.2% (CI 95% 15.9% - 60.3%) in the second week to 55.9% (CI 95% 43.2% - 68.6%) in the sixth week, reaching a plateau (38% - 46%) thereafter. Median (SD) age was 34.2 ( 9.9) years and 205 (69.5%) were female. We did not find significant differences in the prevalence of the most commonly reported underlying medical condition among healthcare workers that tested positive or negative for SARS-CoV-2 infection. After multivariate analysis, using logistic regression, anosmia (OR 4.4 95% C.I. 2.21 - 8.74) and ocular pain (OR 1.95 C.I. 95% 1.14 - 3.33) were the only symptoms independently associated with positivity for SARS-CoV-2 infection. Follow-up information on clinical outcomes showed that 9 (7.2%) healthcare workers were hospitalized (seven were male) and 2 (1.6%) died.Conclusions: The findings of this study confirmed the high burden of SARS-CoV-2 infection among healthcare workers in the hardest hit city by the pandemic in Latin America. Anosmia and ocular pain were symptoms independently associated with COVID-19 diagnosis. In low and middle-income countries, where limited availability of tests is frequent, these findings may contribute to optimize a targeted symptom-oriented screening strategy.
An accomplished procedure of horseradish peroxidase immobilization for removal of acid yellow 11 in aqueous solutions
Authors: Altikatoglu Yapaoz Melda , Attar Azade 
Published: July 2020

Abstract
Horseradish peroxidase (HRP) characteristics were improved by two techniques, Na-alginate entrapment and glutaraldehyde crosslinking prior to alginate entrapment, in order to enhance the stability, functionality and removal of dyes in waste water. Free, entrapped and crosslinked-entrapped enzymes were compared by activity assays, which indicated the optimum temperature is 25 °C and pH 4.0–5.0. Kinetics results showed that alginate entrapment and crosslinking prior to entrapment increased Vmax and did not cause any significant decrease in Km. The thermal resistance of the free enzyme was short-term, zero residual activity after 250 min, while the immobilized enzymes preserved more than 50% of their activity for 5 h at 60 °C. Immobilized HRP was resistant to methanol, ethanol, DMSO and THF. The storage stability of free HRP ended in 35 days whereas entrapped and crosslinked-entrapped HRPs had 87 and 92% residual activity at the 60th day, respectively. HRP was used in the decolorization of azo dye Acid yellow 11 and total decolorization (&gt;99%) was obtained using crosslinked-entrapped HRP. Reusability studies presented the improvement that crosslinked-entrapped HRP reached 74% decolorization after 10 batches. The results demonstrated that the novel immobilized HRP can be used as an effective catalyst for dye degradation of industrial waste effluents.
The Potency of Catechin from Gambir (Uncaria gambir Roxb.) as a Natural Inhibitor of MurA (1UAE) Enzyme: In vitro and In silico studies
Authors: Riyana Bella , Huspa Desi Harneti Putri , Satari Mieke Hemiawati , Kurnia Dikdik 
Published: July 2020

Background:
Currently, infectious diseases caused by pathogenic and resistant bacteria
are more challenging for anti-bacterial drug discovery. The discovery of new anti-bacterial agents
developed in many mechanisms includes disruption of the bacterial cell wall formations. The MurA
is a key enzyme contributing to the first step of bacterial peptidoglycan biosynthesis and is, therefore,
proposed as an effective bactericidal target.

Objective:
The purpose of this research is to identify anti-bacterial compounds from U. gambir
Roxb and to predict the potential inhibitory activities against murA enzyme by in silico study.

Materials and Methods:
Investigation and discovery of new inhibitors of MurA enzyme were conducted
on the medicinal plant of Gambir (Uncaria gambir Roxb) and those that reportedly contained
anti-bacterial agents. The anti-bacterial compounds were isolated by combinations of chromatography
methods guided by anti-bacterial activity against bacteria of E. faecalis, S. mutans, and S.
sanguinis. The structures of active compounds were characterized by spectroscopic methods, and
the anti-bacterial activity was evaluated by the microdilution method (in vitro) combined with molecular
docking of the MurA enzyme (in silico).

Results:
The anti-bacterial flavonoids of catechin were isolated from U. gambir Roxb with MIC
values of 6250 and 12500 ppm, respectively, against S. sanguinis and E. faecalis. The in silico study
showed that catechin has a binding affinity of -8.5 Kcal/mol to MurA which is higher than fosfomycin
as a positive control.

Conclusions:
The catechin is predicted to have potential as a new natural inhibitor of the MurA enzyme
to inhibit bacterial cell wall biosynthesis.
Design and Synthesis of Novel Coumarin Conjugated Acetamides as Promising Anticancer Agents: An In Silico and In Vitro Approach
Authors: Mamatha S.V. , Belagali Shiddappa L. , Bhat Mahesh , Kumbar Vijay M. 
Published: July 2020

Background:
Coumarin and benzophenone possess a vast sphere of biological activities whereas thiazoles
display various pharmacological properties. Hence we focused on incorporation of coumarin and thiazole core to the
benzophenone skeleton to enhance the bioactivity anticipating their interesting biological properties.

Objective:
The objective of the current work is synthesis and biological evaluation of a novel series of coumarin fused
thiazole derivatives.

Methods:
A novel series of Coumarin conjugated thiazolyl acetamide hybrid derivatives were synthesized by multistep
reaction sequence and were characterized by the FT-IR, LCMS and NMR spectral techniques. The newly synthesized
compounds were screened for anticancer activity by in-silico and in-vitro methods. The cytotoxicity of the synthesized
unique compounds had been executed for two different cancer cell lines MCF-7 (Breast cancer) and KB (Oral cancer) in
comparison with standard paclitaxel by MTT assay.

Results:
The compound 7f is a potent motif with an acceptable range of IC50 values, for anti-cancer activity, i.e.,
63.54μg/ml and 55.67μg/ml, against the MCF-7 and KB cell lines, respectively. Molecule docking model revealed
that this compound formed three conventional hydrogen bonds with the active sites of the amino acids,
MET 769, ARG 817, and LYS 721.

Conclusion:
Compound 7f with two methyl groups on the phenoxy ring and one 4-position methoxy group on the benzoyl
ring, showed a significant cytotoxic effect. An advantageous level of low toxicity against normal cell line (L292) by MTT
assay was determined.
Anti-allergy Effect of Sulfated Polysaccharide from Sargassum polycystum on Dinitrofluorobenzene Induced Allergic Contact Dermatitis in Mice
Authors: Dela Cruz Sharmaine Y. , Vasquez Ross D. , Salonga Reginald B. , Corpuz Mary Jho-Anne T. 
Published: July 2020

Background:
Sargassum polycystum C. Agardh has potent antioxidant and antiinflammatory
properties. However, its anti-allergic effect has not yet been reported. In this study, we
investigated the anti-allergic effects of sulfated polysaccharide of S. polycystum (SPSP) in Dinitrofluorobenzene
(DNFB)- induced allergic contact dermatitis animal model.

Methods:
SPSP was extracted through the hot water extraction method and was subjected to compositional
analyses. For the Allergic Contact Dermatitis (ACD) model, symptoms were induced by the
topical application of 0.5% DNFB on the shaved ventral skin of mice. SPSP (500, 1000, and 2000
mg/kg) and Prednisolone were orally administered for seven days after sensitization. Elicitation was
performed seven days later with 0.2% DNFB. After this, ear thickness was measured at baseline and
24 hours post elicitation using a dial thickness gauge. Serum of mice was obtained 24 hours post
elicitation, and the level of IFNγ and TNFα was quantified by enzyme-linked immunosorbent assays
(ELISA).

Results:
SPSP afforded 33.6% carbohydrates, 23.7% sulfate, 7.5% protein, and 1.5% uronic acid
contents. SPSP inhibited the ear swelling and cytokines (IFNγ and TNFα) production in DNFBinduced
mice in a dose-dependent manner. In comparison with Prednisolone (p>0.05), the highest
concentration (2000 mg/kg) of SPSP showed a comparable anti-allergic effect.

Conclusion:
These findings showed that the sulfated polysaccharide from S. polycystum is a potential
natural source to treat allergic contact dermatitis. The effect is attributed to the polysaccharideprotein
complex present in the extract, but further studies are needed to establish the exact mechanism
of action of SPSP in the treatment of the disease.
High Anti-cancer Activity, Low Animal Toxicity, and Structure Activity Relationships of Curcumin Analogs
Authors: Song Sen-Chuan , Mai Yu-Liang , Shi Hua-Hong , Liao Bing , Wang Fei 
Published: July 2020

Background:
Inhibition of cancer cell growth and low in vivo toxicity are two important
criteria for the development of anti-cancer drugs. Curcumin is a promising candidate for developing
novel anti-cancer drug analogs. The research group designed the 3,5-bis-(3,4,5-
trimethoxybenzylidene)-1-methyl-piperidin-4-one analog of curcumin that significantly inhibited
the growth of esophageal cancer cells in vivo. In this study, 81 curcumin analogs were synthesized,
analyzed both in vitro and in vivo, and their structure activity relationships (SARs) were determined.

Methods:
Based on the parent structure of curcumin, 81 analogs of 3,5-bis(substitutedbenzylidene)-
piperidin-4-one compounds were designed and synthesized. Their anti-cancer activity in the human
cancer cell lines was evaluated using the MTT assay, and in vivo toxicity was evaluated in mice.
The SARs of selected compounds were analyzed.

Results and Discussion:
Among the designed curcumin analogs, 61 compounds exerted anti-cancer
effects higher than the parent compound in vitro; 23 compounds inhibited cell growth in the human
cancer cell line at low concentrations (IC50 values below 1 μM). The acute toxicity of curcumin
analogs was tested in mice; 13 compounds were selected, which did not show any obvious toxicity
at doses as high as 25.0 mg/kg. The SARs of these shortlisted curcumin analogs were determined.

Conclusion:
Twenty-three curcumin analogs exhibiting promising in vitro anti-cancer activity and
low in vivo toxicity were designed. SAR analysis indicated the optimal functional groups in the
molecule required for anti-cancer activity. This study not only suggested a useful strategy to design
curcumin analogs for the development of anti-cancer drugs, but also revealed a group of curcumin
analogs which could be further explored.
Frecuencia y altura de corte sobre la calidad del Megathyrsus maximus (cv. Gatton panic)
Authors: Schnellmann Leandro Pablo , Verdoljak Juan José Oscar , Bernadis Aldo , Martínez-Gonzalez Juan Carlos , Castillo-Rod´ríguez Sonia Patricia , Limas-Martínez Andrés Gilberto 
Published: July 2020

El estudio que se presenta a continuación tuvo como objetivo evaluar la frecuencia e intensidad de la calidad nutricional en la materia seca (MS) del pasto Megathyrsus maximus (cv. Gatton panic), y fue desarrollado en la Cabaña Doña Anita, provincia del Chaco, Argentina. Se empleó un diseño completamente al azar con un arreglo factorial de 2 alturas de corte (A = 0,15 y 0,30 m), 3 frecuencias (F = 30, 45 y 90 días) y 2 tiempos (T = 90 y 180 días) con cinco repeticiones para cada uno (60 unidades experimentales). Se determinó el porcentaje de MS, proteína bruta (PB), fibra detergente ácido (FDA), fibra detergente neutra (FDN) y digestibilidad (DI). En el caso de la PB, se encontró una interacción entre las variables A x T y F x T. Para la interacción T x A, los cortes realizados en menor tiempo presentaron diferencia significativa (p < 0,05) en las dos alturas. Para la interacción T x F en la combinación 90 x 30, hay diferencia significativa (p < 0,05) respecto a las restantes combinaciones. Respecto a la FDN, para la variable altura, el contenido de esta se incrementa al aumentar aquella (p < 0,05) debido a un mayor alargamiento de tallos y hojas. En cuanto a la FDA se observaron diferencias (p < 0,05) entre los valores para la interacción T x A para 180 días con 0,15 y 0,30 m. Para la digestibilidad, se encontraron las diferencias significativas (p < 0,05) en la interacción de T x A. Se concluye que el mayor contenido de proteína bruta se obtuvo con la frecuencia de 30 días. La FDN y la FDA aumentaron con el mayor tiempo y la altura de la pastura.
Antioxidant and Antimicrobial Activities of Methanolic Leaves Extract of Lagerstroemia parviflora
Authors: Bharadwaj Namita , Chaturvedi Anjna 
Published: July 2020

An antioxidant is a molecule capable of terminating the chain reactions that damage cells by removing free radical intermediates and inhibit other oxidation reactions by thereby reducing stress responsible for many degenerative disorders. The present study was concerned with phytochemical screening, antioxidant, antimicrobial activities of methanolic leaves extract of Lagerstroemia parviflora. It was observed that the methanol extract showed IC 50 value of 76.05µg/ml as compare to standard ascorbic acid 14.66 µg/ml. It was evident that the extracts showed proton-donating ability and this could serve as free radical inhibitors or scavengers, acting possibly as primary antioxidants. Thus the methanol extract of this plant possesses the strongest ability to scavenge DPPH radical. The leaves extracts of Lagerstroemia parviflora exhibited potential antibacterial activity against Staphylococcus aureus (20±0.2, 21±0.1 and 23±0.1), Salmonella bongori (9±0.3, 12±0.1and 15±0.5) and Aspergillus niger (7±0.1, 9±0.1 and 11±0.2) at the concentration level of 25-100mg/ml. The Results of antimicrobial activity of extract showd more effectiveness against both the selected bacteria (Staphylococcus aureus and Salmonella bongori) found less effective against fungus (Aspergillus niger). The present study suggests that the use of leaves of this plant may be exploited for health supplements and has potential for topical treatment.
Keywords: Lagerstroemia parviflora, Antioxidant, Antimicrobial
AN OVERVIEW ON PHARMACOPOEIAS IN THE WORLD AND MONOGRAPH ELABORATION TECHNIQUES
Authors: YAPAR Evren ALGIN , ÖZDEMİRHAN Mehmet Emre 
Published: July 2020

Pharmacopoeias are official sources that contain national and international rules that must be complied with legally and scientifically regarding substances, materials, drugs, devices and methods used in pharmaceutical field and pharmaceutical production. The monographs are consisted of general titles such as definition, production, characters, identification, tests, assay, storage, labelling and impurities. The World Health Organization states that sixtyeight different pharmacopoeia continue to be used effectively in fifty six countries around the world. In this review information about national and international wold pharmacopoeias, structure and general content of pharmacopoeias, and monograph elaboration techniques are given.
Peer Review History:
Received 26 April 2020; Revised 25 May; Accepted 3 July, Available online 15 July 2020
UJPR follows the most transparent and toughest ‘Advanced OPEN peer review’ system. The identity of the authors and, reviewers will be known to each other. This transparent process will help to eradicate any possible malicious/purposeful interference by any person (publishing staff, reviewer, editor, author, etc) during peer review. As a result of this unique system, all reviewers will get their due recognition and respect, once their names are published in the papers. We expect that, by publishing peer review reports with published papers, will be helpful to many authors for drafting their article according to the specifications. Auhors will remove any error of their article and they will improve their article(s) according to the previous reports displayed with published article(s). The main purpose of it is ‘to improve the quality of a candidate manuscript’. Our reviewers check the ‘strength and weakness of a manuscript honestly’. There will increase in the perfection, and transparency.
Received file

Average Peer review marks at initial stage: 6.0/10
Average Peer review marks at publication stage: 8.0/10
Reviewer(s) detail:
Name: Dr. Mohammed Abdel-Wahab Sayed Abourehab
Affiliation: Umm Al-Qura University; Makkah Al-Mukarramah, Saudi Arabia
E-mail: maabourehab@uqu.edu.sa

Name: Heba M. Abd-El-Azim
Affiliation: Faculty of Pharmacy – Damanhour University
E-mail: h_m_abdelazim@hotmail.com

Comments of reviewer(s):
POLITIK DINASTI DI ACEH; STUDI KEPEMIMPINAN AMPON BANG DI KABUPATEN NAGAN RAYA
Authors: Aklima Aklima , Ramadhan Ilham 
Published: July 2020

The focus of study the political dinasti strategic Ampon Bang of Nagan Raya distric. The aim of reseach to find out the political strategic that strengthens the couse of the political dynasty Ampon Bang aplicatin in Nagan Raya distric.The methodology used is qualitatif methode collecting data throughdocumentation and in dept interview technic. The Result of the research obtained political dynasty leadershipAmpon Bang Usingthe concept of the families with the political strategic carried out; first, leadership organisation structure is filled by family and relatives. Second, close the space for public partisipation.
PERLINDUNGAN HUKUM TERHADAP SUAMI SEBAGAI KORBAN KEKERASAN DALAM RUMAH TANGGA DALAM PANDANGAN HUKUM POSITIF DAN HUKUM ISLAM
Authors: Amrullah Amrullah , Dahliana Dahliana 
Published: July 2020

Abstrak Kekerasan dalam rumah tangga (KDRT) tentang terjadi dalam masyarakat. Korban kekerasan biasanya dari pihak perempuan atau isteri dan anak. Namun, dalam kondisi-kondisi tertentu, suami juga bisa menjadi pihak korban. Penelitian ini secara khusus ingin menkaji tentang pelaksanaan perlindungan hukum bagi suami yang menjadi korban kekerasan. Rumusan masalah penelitian ini adalah bagaimana bentuk hukuman terhadap pelaku KDRT menurut hukum positif dan Hukum Islam, dan bagaimana bentuk perlindungan hukum bagi suami sebagai korban kekerasan dalam rumah tangga dilihat menurut hukum positif dan hukum Islam. Penelitian ini ditulis dengan menggunakan metode kualitatif. Data yang telah dikumpulkan dianalisa dengan cara deskriptif-analisis. Hasil penelitian menunjukkan bentuk hukuman terhadap pelaku KDRT menurut hukum positif ada dua, yaitu hukuman pokok berupa penjara atau denda disesuaikan dengan akibat yang dialami korban. Kemudian hukuman tambahan berupa pembatasan gerak dan hak pelaku. Adapun menurut hukum Islam, bentuk hukuman terhadap pelaku KDRT berupa hukuman qiṣāṣ-diyāt apabila dimungkinkan untuk diterapkan. Apabila tidak ada kemungkinan untuk menerapkannya, maka bentuk hukumannya adalah ta’zīr yang jenis dan bentuk hukumnya sesuai dengan kebijakan pemerintah. Bentuk perlindungan hukum bagi suami sebagai korban kekerasan dalam rumah tangga menurut hukum positif yaitu dalam bentuk upaya pemenu-han hak-hak korban, berupa pelayanan hukum, kesehatan, dan pelayanan psikologis. Bentuk perlindungan tersebut ditetapkan dalam Pasal 10, Pasal 16 ayat (1), Pasal 17, Pasal 21 ayat (1), Pasal 23, Pasal 25, Pasal 35, dan Pasal 36, Undang-Undang Republik Indonesia Nomor 23 Tahun 2004 tentang Penghapusan Kekerasan dalam Rumah Tangga. Sementara dalam hukum Islam, bentuk perlindungan hukum bagi suami sebagai korban kekerasan dalam rumah tangga bisa dalam bentuk adanya peluang bagi suami memberikan pelajaran dan pengajaran kepada isteri, serta Islam memberi peluang bagi setiap orang, baik itu keluarga, masyarakat maupun pemerintah untuk menolong korban dalam bentuk pelayanan hukum, kese-hatan, maupun psikologis. Perlindungan hukum tersebut dinyatakan dalam QS. al-Nisā’ ayat 34, Hadis riwayat Muslim dari Yahya bin Yahya al-Tamimi dan Abu Bakar bin Abi Syaibah. Kata Kunci : Perlindungan Hukum- Suami-Korban KDRT
External Investigators' Follow-Up Intentions When Whistleblowers Report Healthcare Fraud: The Effects of Report Anonymity and Previous Confrontation
Authors: Kaplan Steven E. , Lanier Danny , Pope Kelly R. , Samuels Janet A. 
Published: July 2020

ABSTRACT
Whistleblowing reports, if properly investigated, facilitate the early detection of fraud. Although critical, investigation-related decisions represent a relatively underexplored component of the whistleblowing process. Investigators are responsible for initially deciding whether to follow-up on reports alleging fraud. We report the results of an experimental study examining the follow-up intentions of highly experienced healthcare investigators. Participants, in the role of an insurance investigator, are asked to review a whistleblowing report alleging billing fraud occurring at a medical provider. Thus, participants are serving as external investigators. In a between-participant design, we manipulate the report type and whether the caller previously confronted the wrongdoer. We find that compared to an anonymous report, a non-anonymous report is perceived as more credible and follow-up intentions stronger. We also find that perceived credibility fully mediates the relationship between report type and follow-up intentions. Previous confrontation is not significantly associated with either perceived credibility or follow-up intentions.
Data Availability: Data are available upon request.
APAKAH SAK ETAP MASIH PENTING BAGI AKUNTABILITAS UMKM?
Authors: Widyatama Arif , Sabirin Andi , Ihsan M. , Jarudin Jarudin 
Published: July 2020

Tujuan penelitian ini untuk memahami mengenai penerapan SAK ETAP bagi akuntabilitas UMKM. Penelitian ini merupakan penelitian kualitatif dengan menggunakan paradigm intepretif yaitu dengan mengangkat fenomena atau masalah yang terjadi pada UKM Irwan Bengkel, dengan mengguanakan pendekatan interpretif peneliti ingin melihat pemahaman dari UKM dalam hal ini Irwan Bengkel dalam memahami penerapan SAK ETAP. Usaha Kecil dan Menengah (UKM) Irwan Bengkel belum melakukan pencatatan laporan keuangan sesuai dengan SAK ETAP. Hanya melainkan laporan yang tidak jelas pencatatanya; kedua faktor-faktor yang tidak terlaksananya pencatatan laporan keuangan berbasis (SAK ETAP) pada Usaha Kecil dan Menengah (UKM) Irwan Bengkel, ketiga keberadaan Standar Akuntabilitas Keuangan Entitas Tanpa Publik yang di peruntukan untuk Usaha Kecil dan Mengah tidak dikerahui oleh para pemilik usaha dan kurangnya sumberdaya manusia yang memiliki pemampuan menyusun laporan keuangan. dalam pengambilan keputusan pemilik masih menggunakan intuisi terakhir kurangnya sumberdaya manusia yang dimiliki terutama yang memiliki kemampuan dalam proses penyusunan laporan keuangan.