bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transient Heat-Shock Disrupts Molecular Signals and Competence of Riverine Bubalus Bubalis Oocytes and Early Embryos in a Stage-Specific Manner
Authors: Singh Devender , Kumar Manish , Kumar Rakesh , Datta Tirtha Kumar 
Published: June 2020

Abstract
Background Thermal stress elicits detrimental effect in reproduction performance of Bubalus bubalis, buffalo. Riverine buffalo oocytes and early embryos possess differential ability to grow and survive under thermal stress condition. It is interesting to know how precisely the short and transient heat stress impacts growth and development of buffalo oocytes and early embryos in a stage specific manner. In this study, we aim to identify the most sensitive and vulnerable stage of oocytes and early embryos against transient heat stress as well as unravel the underlying molecular signals responsible for the stunted embryo development under thermal stress. It was assessed by utilizing six different groups of oocytes and embryos with an incorporation of 4 h transient heat shock at 40ºC during different stage of in-vitro maturation (IVM) and in vitro culture (IVC). Results The stressed oocytes of group (grp) 1, 2 and 3 exhibit comparable paces of attaining metaphase-II (M-II) phase at different time interval of IVM. The most detrimental effect of heat stress was observed in grp 2 with sharp reduction in morula to blastocysts transition rate (p<0.05). Expression of mRNA transcripts of HSP8, MnSOD and Sirt-3 genes were significantly increased from mid-IVM to 4-cell embryo although subsequently, down-regulated in embryos of 8-16 cells, morulae and blastocysts. Expression of maternal to embryo transition (MET) genes viz. PAP, U2Af and eIF4A was significantly down regulated from 2-cell embryo to morula (p<0.05). Maternal recognition of pregnancy (MRP) and morulae to blastocyst transition genes were poorly expressed in grp 2 than of the other stress groups (p<0.05). Conclusions Disruption of molecular signal has implicated in the poor formation of inner cell mass (ICM) and trophectoderm (TE) cells, this results in compromised development prospects of early buffalo embryos in a stage specific manner. Well-coordinated molecular signals associated with heat stress held responsible for reduced development of early embryo. We establish the most vulnerable stage of buffalo oocytes and presumptive zygotes against transient heat shock and observe a narrow window as the most critical stage for regulating the development potential of the prospective embryos under short heat stress environment.
Mechanical Force Modulates Periodontal Ligament Stem Cell Characteristics During Bone Remodeling via TRPV4
Authors: Jin Shan-Shan , He Dan-Qing , Wang Yu , Zhang Ting , Yu Hua-Jie , Li Zi-Xin , Zhu Li-Sha , Zhou Yan-Heng , Liu Yan 
Published: June 2020

Abstract
Background: Mechanical force plays an important role in modulating stem cell fate and behaviors, thereby guiding tissue development, homeostasis, and regeneration. However, how periodontal ligament stem cells (PDLSCs) perceive the mechanical stimulus and transfer it into biological signals, and thereby promote alveolar bone remodeling, is unclear. Methods: An animal model of mechanical force-induced tooth movement and a compressive force stimulus in vitro were used in the present study. After force application for 3 and 7 days, the tooth movement distance, the number of mesenchymal stem cells and osteoclasts, and the expression of the proinflammatory cytokines were detected in periodontal tissues. Then, PDLSCs with or without force loading were isolated ex vivo and their stem cell characteristics including clonogenicity, proliferation, multipotent differentiation, and immunoregulatory properties were evaluated. Under the compressive force stimulus in vitro, the effects of the ERK signaling pathway on the PDLSC characteristics were evaluated by Western blotting.Results: Mechanical force in vivo induced PDLSC proliferation, which was accompanied with inflammatory cytokine accumulation, osteoclast differentiation and TRPV4 activation; mechanical force changed the stem cell characteristics of load-induced PDLSCs isolated ex vivo, showing greater clonogenicity and proliferation, reduced differentiation ability, improved induction of macrophage migration, osteoclast differentiation, and proinflammatory factor expression. The biological changes induced by mechanical force could be partially suppressed by a small-molecule antagonist of TRPV4. Mechanistically, the potential communication between mechanical force and biological response through the ERK signaling was activated by TRPV4. Conclusions: Taken together, we show here that the activation of TRPV4 in PDLSCs under mechanical force contributes to the changes in their stem cell characteristics including clonogenicity, proliferation, multipotent differentiation, and immunoregulation and modulates bone remodeling during tooth movement.
“A false sense of confidence” The perceived role of inflammatory point-of-care testing in managing urinary tract infections in Dutch nursing homes: a qualitative study
Authors: Kuil Sacha Danielle , Schneeberger Caroline , Leth Frank van , de Jong Menno , Harting Janneke 
Published: June 2020

Abstract
BackgroundDiagnosing urinary tract infections (UTI) in nursing home residents is complex, due to frequent non-specific symptomatology and asymptomatic bacteriuria. The objective of this study was to explore health care professionals’ perceptions of the proposed use of inflammatory marker Point-Of-Care Testing (POCT) in this respect.MethodsWe conducted a qualitative inquiry (2018 - 2019) alongside the multicenter PROGRESS study (NL6293), which assessed the sensitivity of C-reactive protein and procalcitonin POCT in UTI. We used semi-structured face-to-face interviews. The participants were physicians (n=12) and nurses (n=6) from 13 nursing homes in the Netherlands. Most respondents were not familiar with inflammatory marker POCT, while some used POCT for respiratory tract infections. Both the interview guide and the analysis of the interview transcripts were based on the Consolidated Framework for Implementation Research.Results All respondents acknowledged that sufficiently sensitive POCT could decrease diagnostic uncertainty to some extent in residents presenting with non-specific symptoms. They primarily thought that negative test results would rule out UTI and justify withholding antibiotic treatment. Secondly, they described how positive test results could rule in UTI and justify antimicrobial treatment. However, most respondents also expected new diagnostic uncertainties to arise. Firstly, in case of negative test results, they were not sure how to deal with residents’ persisting non-specific symptoms. Secondly, in case of positive test results, they feared overlooking infections other than UTI. These new uncertainties could lead to inappropriate antibiotics use. Therefore, POCT was thought to create a false sense of confidence. Conclusions Our study suggests that inflammatory marker POCT will only improve UTI management in nursing homes to some extent. To realize the expected added value, any implementation of POCT requires thorough guidance to ensure appropriate use. Developing UTI markers with high negative and positive predictive values may offer greater potential to improve UTI management in nursing homes.
Coronavirus Disease 2019 (COVID-19) in Italy: Double reading of chest CT examination
Authors: Alfonso Reginelli , Roberto Grassi , Beatrice Feragalli , Paola Belfiore Maria , Alessandro Montanelli , Gianluigi Patelli , Michelearcangelo La Porta , Fabrizio Urraro , Fusco Roberta , Vincenza Granata , Antonella Petrillo , Giuliana Giacobbe , Maria Russo Gaetano , Palmino Sacco , Roberta Grassi , Salvatore Cappabianca 
Published: June 2020

Abstract
OBJECTIVETo assess the performance of the second reading of chest Compute Tomography (CT) examinations by expert radiologists in patients with discordance between the reverse transcription real-time fluorescence polymerase chain reaction (RT-PCR) test for COVID-19 viral pneumonia and the first CT report.MATERIALS AND METHODS.Three hundred seventy-heigth patients were included in this retrospective study (121 women and 257 men; 71 years of median age - range, 29–93 years) subjected to RT-PCR test for suspicious COVID-19 infection. All patients were subjected to CT examination in order to evaluate the pulmonary disease involvement by COVID-19. CT images were reviewed first by two radiologists who identified COVID-19 typical CT patterns and then reanalyzed by anoter two radiologists using a CT structured report for COVID-19 diagnosis.RESULTS.The median temporal window between RT-PCRs execution and CT scan was 0 days with a range of [-9, 11] days. RT-PCR test was resulted positive in 328/378 (86.8%). Discordance between RT-PCR and CT findings for viral pneumonia was revealed in 60 cases. The second reading changed the CT diagnosis in 16/60 (26.7%) cases contributing to increase the concordance with the RT-PCR. Among these 60 cases, 8 were false negative with positive RT-PCR, and 36 were false positive with negative RT-PCR. Sensitivity, specificity, positive predictive value and negative predictive value of CT were respectively of 97.3%, 53.8%, 89.0%, and 88.4%.CONCLUSION.Double reading of CT could increase the diagnostic confidence of radiological interpretation in COVID-19 patients. Using expert second readers could reduce the rate of discrepant cases between RT-PCR results and CT diagnosis for COVID-19 viral pneumonia.
Active Vibration Isolation for A Parallel Wheel-legged Robot Based on Dynamic Model
Authors: Guo Fei , Wang Shoukun , Yue Binkai , Wang Junzheng 
Published: June 2020

Abstract
Serving Stewart plat as wheel-legged construction, the most outstanding superiority of proposed wheel-legged hybrid robot (WLHR) is active vibration isolation during rolling on rugged terrain. This paper presents a force-driven control approach based on model predictive control (MPC) to design optimal control input for Stewart parallel wheel-leg that locomotes using swing foot trajectory. Adding adaptive impedance control in outermost loop, controlling framework prevents robot body horizontal and from vibration over rolling motion. Through dynamic model of Stewart mechanism, controller first creates predictive model by combining Newton-Euler equation, Newton-Raphson iteration of forward kinematic solving for current configuration, inverse kinematic calculation of Stewart obtaining desired joint position, and Gain/Integration module determining reference torque. With minimizing control deviation and input as objective function, a novel control optimization formulation generates optimum input for each control duration. These actuating force naturally enables each strut stretching and retracting used to realize six degree-of-freedom (6DOF) motion for Stewart wheel-leg. We exploit the variable-adapting method to reasonably adjust environmental impedance parameters by current position, velocity, force feedback of wheel-leg. This allow us to adequately acknowledge the desired support force tracking, isolating robot from isolation that is generated from unknown terrain. We demonstrate the validation of our control methodology on physical prototype by tracking a Bezier curve and active vibration isolation while the robot is rolling on decelerate strip. Respectively given PI controller and a sort of traditional impedance controller as comparison, a better performance of proposed algorithm was operated and evaluated through displacement and force sensors internally-installed in each cylinder, as well as IMU mounted on robot body.
Optimization Of Real-Time Reverse Transcriptase Polymerase Chain Reaction For Detection Of Dengue Virus
Authors: Azizi Kaunara Ally , Ndaro Arnold J , Maro Athanasia , Saro Adonira T , Kavishe Reginald 
Published: June 2020

Abstract
Objective Rapid and accurate laboratory confirmatory is very essential for control measures of dengue virus infections. However, many cases of dengue virus infections in most of the hospitals remain undiagnosed due to presence of other febrile illnesses with overlapping symptoms and lack of specificity in most of laboratory diagnostic methods. This study was set to optimize conditions for real time reverse transcriptase polymerase chain reaction (RT-PCR) for detection of dengue virus by using rapid and simple nucleic acid extraction method.Results The real time RT-PCR technique was successfully optimized using simple and rapid method for purification of nucleic acid, ‘boom method’. The technique works better when performed in a two-step procedure and can works well with all range of real time PCR machines. The optimized real time RT-PCR used in the present study is a valuable and reliable technique for routine diagnosis of dengue. Further investigation on the cost effectiveness in adopting this technique for routine screening and monitoring of the dengue infection should be done.
Frequency Ratio Density, Logistic Regression and Weights of Evidence Modelling for Landslide Susceptibility Assessment and Mapping in Yanase and Naka Catchments of Southeast Shikoku, Japan
Authors: Meten Matebie , Bhandary Netra Prakash 
Published: June 2020

Abstract
Landslide susceptibility mapping is an important tool for disaster management and development activities such as planning of transportation infrastructure, settlement and agriculture. Shikoku Island, which is found in the southwest of Japan, is one of the most landslide prone areas because of heavy typhoon rainfall, complex geology and the presence of mountainous areas and low topographic features (valleys).Yanase and Naka Catchments of Shikoku Island in Japan were chosen as a study area. Frequency Ratio Densisty (FRD), Logistic Regression (LR) and Weights of Evidence (WoE) models were applied in a GIS environment to prepare the landslide susceptibility maps of this area. Data layers including slope, aspect, profile curvature, plan curvature, lithology, land use, distance from river, distance from fault and annual rainfall were used in this study. In FR method, two models were attempted but the FRD model was found slightly better in its performance. In case of LR method, two models, one with equal proportion and the other with unequal proportion of landslide and non-landslide points were carried out and the one with equal proportions was chosen based on its highest performance. A total of five landslide susceptibility maps(LSMs) were produced using FR, LR and WoE models with two, two and one were attempted respectively. However, one best model was chosen from the FR and LR methods based on the highest area under the curve (AUC) of the receiver operating characteristic (ROC) curves. This reduced the total number of landslide susceptibility maps to three with the success rates of 86.7%, 86.8% and 80.7% from FRD, LR and WoE models respectively. For validation purpose, all landslides were overlaid over the three landslide susceptibility maps and the percentage of landslides in each susceptibility class was calculated. The percentages of landslides that fall in the high and very high susceptibility classes of FRD, LR and WoE models showed 82%, 84% and 78% respectively. This showed that the LR model with equal proportions of landslides and non-landslide points is slightly better than FRD and WoE models in predicting the future probability of landslide occurrence.
Is high-grade endometrial stromal sarcoma the key to disease prognosis in cases of coexisting leiomyosarcoma? A case report
Authors: Ling Feng , Ruan Sibei , Xiong XiaoMing , Li Na , Zhao DongMei , Zhang Cuiwei 
Published: June 2020

Abstract
Background:The uterine leiomyosarcoma combined high-grade endometrial stromal sarcoma (ESS) is quite rare.Case presentation: We reported such a case in which high-grade ESS with BCOR gene alterations and leiomyosarcoma coexist in a patient. And, most impressively, high-grade ESS with BCOR gene alterations caused ovarian and pelvic metastases, although its volume was less than 1% of leiomyosarcoma.Conclusions: We suggested that when both high-grade ESS BCOR gene alterations and leiomyosarcoma are present in a patient, the high-grade ESS needs to be noted in the pathological report even if it accounts for less than 1% of the tumour mass.
Integrative transcriptome analyses empower the anti-COVID-19 drug arsenal
Authors: El-Hachem Nehme , Eid Edward , Nemer Georges , Dbaibo Ghassan , Rubeiz Nelly , Zeineldine Salah , Matar Ghassan M. , Bikorimana Jean-Pierre , Shammaa Riam , Haibe-Kains Benjamin , Kurban Mazen , Rafei Moutih 
Published: June 2020

Abstract
The beginning of the twenty-first century has been marked by three distinct waves of zoonotic coronavirus outbreaks into the human population. The current pandemic COVID-19 (Coronavirus disease 2019) is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). With a rapid infection rate, it is a global threat endangering the livelihoods of millions worldwide. Currently, and despite the collaborative efforts of governments, researchers, and the pharmaceutical industries, there are no substantially significant treatment protocols for the disease. To address the need for such an immediate call of action, we leveraged the largest dataset of drug-induced transcriptomic perturbations, public SARS-CoV-2 transcriptomic datasets, and expression profiles from normal lung transcriptomes. Our unbiased systems biology approach not only shed light on previously unexplored molecular details of SARS-CoV-2 infection (e.g., interferon signaling, inflammation and ACE2 co-expression hallmarks in normal and infected lungs) but most importantly prioritized more than 50 repurposable drug candidates (e.g., Corticosteroids, Janus kinase and Bruton kinase inhibitors). Further clinical investigation of these FDA approved candidates as monotherapy or in combination with an antiviral regimen (e.g., Remdesivir) could be promising treatment options against COVID-19.
Impact of COVID-19 pandemic and the lockdown on invasive meningococcal disease
Authors: Taha Muhamed-Kheir , Deghmane Ala-Eddine 
Published: June 2020

Abstract
Objective: Few data are available on the association between SARS-CoV-2 and secondary bacterial infections. Such an association was described for flu and invasive meningococcal disease (IMD). We aimed exploring such a correlation between COVID-19and IMD.Results: We compared IMD cases received at the French National Reference Centre for meningococci and Haemophilus influenzae that are sent as part of the mandatory reporting of IMD. We compared these data during the period 01 January-15 May 2020 to those from the same period in 2019. IMD cases that were associated with respiratory presentations significantly increased in 2020, involved elderly and were due to unusual isolates. However, usual IMD cases due to highly transmissible isolates decreased during the lockdown. Enhancing IMD surveillance and anti-meningococcal vaccination in elderly should to be addressed.
Long Noncoding RNA LPP-AS2 Promotes Glioma Tumorigenesis via miR-7-5p/EGFR/PI3K/AKT/c-MYC Feedback Loop
Authors: Zhang Xiaoming , Niu Wanxiang , Mu Maolin , Hu Shanshan , Niu Chaoshi 
Published: June 2020

Abstract
Background: Glioma is the most common primary malignant intracranial tumor with poorly clinical prognosis in adults. Accumulating evidences indicate that long non-coding RNAs (lncRNAs) have served as important regulators in cancer progression, including glioma. Here, we identified a new lncRNA LPP antisense RNA-2 (LPP-AS2) and investigated its function and mechanism in the occurrence and development of glioma.Methods: High-throughput RNA sequencing was performed to discriminate the differentially expression lncRNAs and mRNAs between glioma tissues and normal brain tissues. The expression of LPP-AS2, epidermal growth factor receptor (EGFR) and miR-7-5p in glioma tissues and cell lines were detected by real-time quantitative PCR (RT-qPCR). The functions of lncRNA LPP-AS2 in glioma were measured by in vivo and in vitro assays. Insights of the underlying mechanism of competitive endogenous RNAs (ceRNAs) were originated from bioinformatic analysis, dual luciferase reporter assays, RNA pulldown assays, RNA immunoprecipitation (RIP) and rescue experiments. Results: The results of high-throughput RNA-seq indicated that lncRNA LPP-AS2 was upregulated in glioma tissues and further confirmed by RT-qPCR. Higher LPP-AS2 expression was related to poor prognosis of glioma patients. Functional studies illustrated that LPP-AS2 depletion inhibited glioma cell proliferation, invasion and promoted apoptosis in vitro and restrained tumor growth in vivo, whereas overexpression of LPP-AS2 resulted in opposite effects. In addition, LPP-AS2 and EGFR were observed of co-expression networks, and LPP-AS2 functioned as a ceRNA to regulate EGFR expression by sponging miR-7-5p in glioma cells. Result of chromatin immunoprecipitation (ChIP) assay validated that c-MYC was directly bind with promoter region of LPP-AS2. As a downstream protein of EGFR, c-MYC was modulated by LPP-AS2 and in turn increased LPP-AS2 expression. Thus, lncRNA LPP-AS2 promoted glioma tumorigenesis via a miR-7-5p/EGFR/PI3K/AKT/c-MYC feedback loop. Conclusions: Our study elucidated that LPP-AS2 acted as an oncogene through a novel molecular pathway in glioma and might be a potential therapeutic approach for glioma diagnosis, therapy and prognosis.
Adverse Effects of Total Hip Arthroplasty on The Hip Abductor and Adductor Muscle Lengths and Moment Arms During Gait
Authors: Hu Xiangjun , Zheng Nan , Hsu Wei-Chun , Zhang Jingwei , Li Huiwu , Chen Yunsu , Dai Kerong , Tsai Tsung-Yuan 
Published: June 2020

Abstract
Background: Precise evaluation the hip abductor and adductor muscles function in total hip arthroplasty (THA) patients during gait could help prevent postoperative complications and optimize the rehabilitation training program. The purpose of this study was to elucidate the effects of THA on the hip abductor and adductor muscle lengths and moment arms of in vivo patients during gait.Methods: Ten unilateral THA patients received CT scan and dual fluoroscopic imaging for the hip kinematics during gait. The hip abductor and adductor muscle insertions were digitized on the 3D hip model for the determination of their dynamic lines of action and moment arms. Changes in the hip abductor and adductor muscle lengths and moment arms of THA patients between the implanted and non-implanted sides were quantified during gait.Results: The adductor longus, adductor brevis, and pectineus of the implanted hips had significantly (P<0.05) less elongation than that of the non-implanted side during the stance phase. The gluteus medius, gluteus minimus, and piriformis moment arms of the implanted side were significantly shorter. The piriformis muscle moment arm was significantly larger. In the double support phase, the adductor magnus and adductor longus moment arms of the implanted sides were significantly decreased. Conclusions: Results suggested that the adverse effects of THA on hip stability. Development of a rehabilitation program considering the effects of THA is essential. Accurate surgical techniques may reduce the impact of THA on the peripheral muscles.