bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multi‐“Color” Delineation of Bone Microdamages Using Ligand‐Directed Sub‐5 nm Hafnia Nanodots and Photon Counting CT Imaging
Authors: Ostadhossein Fatemeh , Tripathi Indu , Benig Lily , LoBato Denae , Moghiseh Mahdieh , Lowe Chiara , Raja Aamir , Butler Anthony , Panta Raj , Anjomrouz Marzieh , Chernoglazov Alex , Pan Dipanjan 
Published: November 2019

AbstractThe early detection of bone microdamages is crucial to make informed decisions about the therapy and taking precautionary treatments to avoid catastrophic fractures. Conventional computed tomography (CT) imaging faces obstacles in detecting bone microdamages due to the strong self‐attenuation of photons from bone and poor spatial resolution. Recent advances in CT technology as well as novel imaging probes can address this problem effectively. Herein, the bone microdamage imaging is demonstrated using ligand‐directed nanoparticles in conjunction with photon counting spectral CT. For the first time, Gram‐scale synthesis of hafnia (HfO2) nanoparticles is reported with surface modification by a chelator moiety. The feasibility of delineating these nanoparticles from bone and soft tissue of muscle is demonstrated with photon counting spectral CT equipped with advanced detector technology. The ex vivo and in vivo studies point to the accumulation of hafnia nanoparticles at microdamage site featuring distinct spectral signal. Due to their small sub‐5 nm size, hafnia nanoparticles are excreted through reticuloendothelial system organs without noticeable aggregation while not triggering any adverse side effects based on histological and liver enzyme function assessments. These preclinical studies highlight the potential of HfO2‐based nanoparticle contrast agents for skeletal system diseases due to their well‐placed K‐edge binding energy.
Aqueously Cathodic Deposition of ZIF‐8 Membranes for Superior Propylene/Propane Separation
Authors: Wei Ruicong , Chi Heng‐Yu , Li Xiang , Lu Dongwei , Wan Yi , Yang Chih‐Wen , Lai Zhiping 
Published: November 2019

AbstractElectrochemical deposition has emerged as a novel approach to fabricate metal–organic framework (MOF) films. Here, for the first time, an aqueously cathodic deposition (ACD) approach is developed to fabricate ZIF‐8 type of MOF membranes without addition of any supporting electrolyte or modulator. The fabrication process uses 100% water as the sole solvent and a low‐defect density membrane is obtained in only 60 min under room temperature without any pre‐synthesis treatment. The membrane exhibits superior performance in C3H6/C3H8 separation with 182 GPU C3H6 permeance and 142 selectivity, making it sit at the upper bound of permeance versus selectivity graph, outperforming majority of the published data up to 2019. Notably, this approach uses an extremely low current density (0.13 mA cm−2) operated under an ultrafacile apparatus set‐up, enabling an attractive way for environmentally friendly, energy efficient, and easily scalable MOF membrane fabrications. This work demonstrates a great potential of aqueously electrochemical deposition of MOF membrane in the future research.
ROS‐Mediated Apoptosis and Anticancer Effect Achieved by Artesunate and Auxiliary Fe(II) Released from Ferriferous Oxide‐Containing Recombinant Apoferritin
Authors: Ji Peng , Huang Haiqin , Yuan Shirui , Wang Le , Wang Siqi , Chen Yiwei , Feng Na , Veroniaina Hanitrarimalala , Wu Ziheng , Wu Zhenghong , Qi Xiaole 
Published: November 2019

AbstractReactive oxygen species (ROS)‐mediated apoptosis is considered a crucial therapeutic mechanisms for artesunate (AS). As an Fe(II)‐dependent drug, the anticancer effect of AS is often limited due to insufficient Fe(II) concentration in targeted cells. To overcome this problem, a recombinant apoferritin nanocarrier containing ferriferous oxide (M‐HFn) is constructed to produce auxiliary exogenous Fe(II) when delivering AS to cancer cells. Here, the newly fabricated AS‐loaded M‐HFn nanoparticles (M‐HFn@AS NPs) can significantly improve the tumor‐specific targeting and intracellular uptake efficiency of AS in human cervical carcinoma cells. After being captured in the acidic cavity of endosomes, M‐HFn@AS NPs can simultaneously release Fe(II) and allow AS to activate satisfactory ROS‐mediated apoptosis. Furthermore, in vivo studies demonstrate that M‐HFn@AS NPs can selectively accumulate in tumors to efficiently inhibit tumor growth. Thus, M‐HFn@AS NPs are a promising system to enhance the therapeutic effect of Fe(II)‐dependent drugs.
Aggregation‐Induced Dual‐Phosphorescence from Organic Molecules for Nondoped Light‐Emitting Diodes
Authors: Wang Tao , Su Xiaoge , Zhang Xuepeng , Nie Xiancheng , Huang Linkun , Zhang Xingyuan , Sun Xiang , Luo Yi , Zhang Guoqing 
Published: November 2019

AbstractAggregation‐induced emission (AIE) is a beneficial strategy for generating highly effective solid‐state molecular luminescence without suffering losses in quantum yield. However, the majority of reported AIE‐active molecules exhibit only strong fluorescence, which is not ideal for electrical excitation in organic light‐emitting diodes (OLEDs). By introducing various substituent groups onto the biscarbazole compound, a series of molecular materials with aggregation‐induced phosphorescence (AIP) is designed, which exhibits two distinctly different phosphorescence bands and an absolute solid‐state room‐temperature phosphorescence quantum yield up to 64%. Taking advantage of the AIE feature, the AIP molecules are fabricated into OLEDs as a homogeneous light‐emitting layer, which allows for relatively small efficiency roll‐off and shows an external electroluminescence quantum yield of up to 5.8%, more than the theoretical limit for purely fluorescent OLED devices. The design showcases a promising strategy for the production of cost‐effective and highly efficient OLED technology.
A Nacre‐Inspired Separator Coating for Impact‐Tolerant Lithium Batteries
Authors: Song Yong‐Hui , Wu Kai‐Jin , Zhang Tian‐Wen , Lu Lei‐Lei , Guan Yong , Zhou Fei , Wang Xiu‐Xia , Yin Yi‐Chen , Tan Yi‐Hong , Li Feng , Tian Te , Ni Yong , Yao Hong‐Bin , Yu Shu‐Hong 
Published: November 2019

AbstractThe commercial ceramic nanoparticle coated microporous polyolefin separators used in lithium batteries are still vulnerable under external impact, which may cause short circuits and consequently severe safety threats, because the protective ceramic nanoparticle coating layers on the separators are intrinsically brittle. Here, a nacre‐inspired coating on the separator to improve the impact tolerance of lithium batteries is reported. Instead of a random structured ceramic nanoparticle layer, ion‐conductive porous multilayers consisting of highly oriented aragonite platelets are coated on the separator. The nacre‐inspired coating can sustain external impact by turning the violent localized stress into lower and more uniform stress due to the platelet sliding. A lithium‐metal pouch cell using the aragonite platelet coated separator exhibits good cycling stability under external shock, which is in sharp contrast to the fast short circuit of a lithium‐metal pouch cell using a commercial ceramic nanoparticle coated separator.
MoN Supported on Graphene as a Bifunctional Interlayer for Advanced Li‐S Batteries
Authors: Tian Da , Song Xueqin , Wang Maoxu , Wu Xian , Qiu Yue , Guan Bin , Xu Xianzhu , Fan Lishuang , Zhang Naiqing , Sun Kening 
Published: November 2019

AbstractRational design of effective polysulfide barriers is highly important for high‐performance lithium‐sulfur (Li‐S) batteries. A variety of adsorbents have been applied as interlayers to alleviate the shuttle effect. Nevertheless, the unsuccessful oxidation of Li2S on interlayers leads to loss of active materials and blocks Li ion transport. In this work, a MoN‐based interlayer sandwiched between the C‐S cathode and the separator is developed. Such an interlayer not only strongly binds lithium polysulfides via Mo‐S bonding but also efficiently accelerates the decomposition of Li2S. The acceleration mechanism toward Li2S decomposition is determined to be a combination of contributions of catalytic cleavage of Li‐S bond in Li2S based on the proposed covalence‐activation mechanism and rapid migration of the produced Li ions. As a result, the C–S cathodes with the as‐developed interlayer manifest a negligible charging potential barrier and outstanding cycling stability with a very low capacity fading rate of 0.023% per cycle during 1500 cycles at 1 C. High areal capacity of 6.02 mAh cm−2 is achieved for high sulfur loading of 7.0 mg cm−2 after cycling at 0.1 C. The material and strategy demonstrated in this work can open the door toward developing shuttle suppression interlayers without impairing cathode performance.
Exosomal microRNA in peritoneal fluid as a biomarker of peritoneal metastases from gastric cancer
Authors: Ohzawa Hideyuki , Kumagai Yuko , Yamaguchi Hironori , Miyato Hideyo , Sakuma Yasunaru , Horie Hisanaga , Hosoya Yoshinori , Kawarai Lefor Alan , Sata Naohiro , Kitayama Joji 
Published: November 2019

AbstractAimPeritoneal metastases (PM) frequently occur in patients with gastric cancer and result in a poor prognosis. Exosomes play pivotal roles in tumor metastasis through the transfer of microRNAs (miRNAs). We examined the exosomal miRNA profile in peritoneal fluids to identify novel biomarkers to reflect tumor burden in the peritoneum.MethodsExosomes were isolated from peritoneal fluids of patients of gastric cancer with macroscopic (P1) or microscopic (P0CY1) peritoneal metastasis (PM) and comprehensive miRNA expression analysis was carried out. Expressions of candidate miRNAs were then validated in all 58 samples using TaqMan Advanced miRNA Assays.ResultsIn initial screening, we carried out comprehensive analysis of exosomal miRNA using peritoneal fluids from 11 and 14 patients with or without PM, respectively, and identified 11 dysregulated miRNAs in PM (+) samples. Validation analysis showed that four miRNAs (miR‐21‐5p, miR‐92a‐3p, miR‐223‐3p, and miR‐342‐3p) were significantly upregulated in 12 PM (+) samples, and their expression levels showed positive correlation with peritoneal cancer index. In contrast, miR‐29 family were all downregulated in patients with PM (+) samples. Moreover, in 24 patients with pT4 tumor, miR‐29 at gastrectomy tended to be lower in six patients with peritoneal recurrence with significant differences in miR‐29b‐3p (P = .012).ConclusionExpression pattern of miRNAs in peritoneal exosomes well reflects the tumor burden in the peritoneal cavity and could be a useful biomarker in the treatment of PM.
High expression of toxic Streptomyces phospholipase D in Escherichia coli under salt stress and its mechanism
Authors: Xiong Weide , Luo Weiyi , Zhang Xueliang , Pan Xueshan , Zeng Xianhai , Yao Chuanyi , Jing Keju , Shen Liang , Chen Cuixue , Ling Xueping , Lu Yinghua 
Published: November 2019

AbstractPhospholipase D (PLD) cytotoxicity limits its high heterologous expression and further application in the food industry. The specific physiological consequences of highly expressed PLD in vivo are unclear. In this study, we observed that PLD expression by Escherichia coli caused phosphatidic acid accumulation and membrane permeability enhancement, characterized by the disruption of ionic homeostasis with enhanced K+ efflux and Na+ influx, which eventually may result in cell injury or death. Moreover, we found that cations (Na+, K+, Li+, or Mg2+) stress alleviated cell growth inhibition and profoundly increased PLD production. A large quantity of PLD (1.1 × 106 U/L) was obtained in a bioreactor. The mechanism for Na+/K+‐induced protection may be the improvement of oxidative phosphorylation through positive changes in membrane potential via redistribution of Na+/K+ inside and outside the cell, which was accompanied by enhanced Na+ efflux. Combined, these findings provided novel insights for PLD function and PLD production.
A 30‐Year Clinical and Magnetic Resonance Imaging Observational Study of Multiple Sclerosis and Clinically Isolated Syndromes
Authors: Chung Karen K. , Altmann Daniel , Barkhof Frederik , Miszkiel Katherine , Brex Peter A. , O'Riordan Jonathan , Ebner Michael , Prados Ferran , Cardoso M. Jorge , Vercauteren Tom , Ourselin Sebastien , Thompson Alan , Ciccarelli Olga , Chard Declan T. 
Published: November 2019

ObjectiveClinical outcomes in multiple sclerosis (MS) are highly variable. We aim to determine the long‐term clinical outcomes in MS, and to identify early prognostic features of these outcomes.MethodsOne hundred thirty‐two people presenting with a clinically isolated syndrome were prospectively recruited between 1984 and 1987, and followed up clinically and radiologically 1, 5, 10, 14, 20, and now 30 years later. All available notes and magnetic resonance imaging scans were reviewed, and MS was defined according to the 2010 McDonald criteria.ResultsClinical outcome data were obtained in 120 participants at 30 years. Eighty were known to have developed MS by 30 years. Expanded Disability Status Scale (EDSS) scores were available in 107 participants, of whom 77 had MS; 32 (42%) remained fully ambulatory (EDSS scores ≤3.5), all of whom had relapsing–remitting MS (RRMS), 3 (4%) had RRMS and EDSS scores >3.5, 26 (34%) had secondary progressive MS (all had EDSS scores >3.5), and MS contributed to death in 16 (20%). Of those with MS, 11 received disease‐modifying therapy. The strongest early predictors (within 5 years of presentation) of secondary progressive MS at 30 years were presence of baseline infratentorial lesions and deep white matter lesions at 1 year.InterpretationThirty years after onset, in a largely untreated cohort, there was a divergence of MS outcomes; some people accrued substantial disability early on, whereas others ran a more favorable long‐term course. These outcomes could, in part, be predicted by radiological findings from within 1 year of first presentation. ANN NEUROL 2020;87:63–74
Self‐Reported Head Trauma Predicts Poor Dual Task Gait in Retired National Football League Players
Authors: Manor Brad , Zhou Junhong , Lo On‐Yee , Zhu Hao , Gouskova Natalia A. , Yu Wanting , Zafonte Ross , Lipsitz Lewis A. , Travison Thomas G. , Pascual‐Leone Alvaro 
Published: November 2019

ObjectiveSymptomatic head trauma associated with American‐style football (ASF) has been linked to brain pathology, along with physical and mental distress in later life. However, the longer‐term effects of such trauma on objective metrics of cognitive–motor function remain poorly understood. We hypothesized that ASF‐related symptomatic head trauma would predict worse gait performance, particularly during dual task conditions (ie, walking while performing an additional cognitive task), in later life.MethodsSixty‐six retired professional ASF players aged 29 to 75 years completed a health and wellness questionnaire. They also completed a validated smartphone‐based assessment in their own homes, during which gait was monitored while they walked normally and while they performed a verbalized serial‐subtraction cognitive task.ResultsParticipants who reported more symptomatic head trauma, defined as the total number of impacts to the head or neck followed by concussion‐related symptoms, exhibited greater dual task cost (ie, percentage increase) to stride time variability (ie, the coefficient of variation of mean stride time). Those who reported ≥1 hit followed by loss of consciousness, compared to those who did not, also exhibited greater dual task costs to this metric. Relationships between reported trauma and dual task costs were independent of age, body mass index, National Football League career duration, and history of musculoskeletal surgery. Symptomatic head trauma was not correlated with average stride times in either walking condition.InterpretationRemote, smartphone‐based assessments of dual task walking may be utilized to capture meaningful data sensitive to the long‐term impact of symptomatic head trauma in former professional ASF players and other contact sport athletes. ANN NEUROL 2020;87:75–83
RETRACTED: Huaier extract suppresses non‐small cell lung cancer progression through activating NLRP3‐dependent pyroptosis
Authors: Xie Jun , Zhuan Bing , Wang Haixia , Wang Yan , Wang Xi , Yuan Qun , Yang Zhao 
Published: November 2019

AbstractRecent studies have reported the anticancer activity of huaier extract in various human malignancies. However, little is known about the effect of huaier extract in non‐small cell lung cancer (NSCLC) and its underlying mechanism. The current study aimed to investigate whether huaier extract affects the progression of NSCLC. mRNA and proteins expression of pyroptotic‐related genes (NLRP3, caspase‐1, IL‐1β, and IL‐18) in NSCLC tissues and cells were, respectively, detected by qRT‐PCR and western blot. The effects of huaier extract on NSCLC cell viability and cytotoxicity were evaluated by CCK‐8 assay, colony formation assay, and LDH detection kit. Besides, we established a xenograft model to assess the antitumor effect of huaier extract on tumor growth in vivo. Our results showed that the expression of pyroptotic‐related genes was downregulated in NSCLC tissues and cell lines. Huaier extract pretreatment inhibited cell viability and the percentage of colony formation of H520 and H358 cells, and upregulated the expression of pyroptotic‐related genes. Mechanistically, huaier extract exhibited antitumor effect in NSCLC via inducing NLRP3‐dependent pyroptosis in vitro and in vivo. In conclusion, our finding confirmed that huaier extract played an antitumor role in NSCLC progression through promoting pyroptotic cell death, which provided a new potential strategy for NSCLC clinical treatment.
Combination mode of antimalarial drug mefloquine and human serum albumin: Insights from spectroscopic and docking approaches
Authors: Musa Kabiru A. , Ridzwan Nor F. W. , Mohamad Saharuddin B. , Tayyab Saad 
Published: November 2019

AbstractThe interaction between mefloquine (MEF), the antimalarial drug, and human serum albumin (HSA), the main carrier protein in blood circulation, was explored using fluorescence, absorption, and circular dichroism spectroscopic techniques. Quenching of HSA fluorescence with MEF was characterized as static quenching and thus confirmed the complex formation between MEF and HSA. Association constant values for MEF‐HSA interaction were found to fall within the range of 3.79‐5.73 × 104 M˗1 at various temperatures (288, 298, and 308 K), which revealed moderate binding affinity. Hydrogen bonds and hydrophobic interactions were predicted to connect MEF and HSA together in the MEF‐HSA complex, as deduced from the thermodynamic data (ΔS = +133.52 J mol−1 K−1 and ΔH = +13.09 kJ mol−1) of the binding reaction and molecular docking analysis. Three‐dimensional fluorescence spectral analysis pointed out alterations in the microenvironment around aromatic amino acid (tryptophan and tyrosine) residues of HSA consequent to the addition of MEF. Circular dichroic spectra of HSA in the wavelength ranges of 200‐250 and 250‐300 nm hinted smaller changes in the protein's secondary and tertiary structures, respectively, induced by MEF binding. Noncovalent conjugation of MEF to HSA bettered protein thermostability. Site marker competitive drug displacement results suggested HSA Sudlow's site I as the MEF binding site, which was also supported by molecular docking analysis.
Can sustainable investments outperform traditional benchmarks? Evidence from global stock markets
Authors: Cunha Felipe Arias Fogliano de Souza , de Oliveira Erick Meira , Orsato Renato J. , Klotzle Marcelo Cabus , Cyrino Oliveira Fernando Luiz , Caiado Rodrigo Goyannes Gusmão 
Published: November 2019

AbstractTo contribute to overcoming global sustainability challenges, investors have been increasingly interested in making sustainable investments and incorporating environmental, social and governance (ESG) criteria into their portfolio selection decisions and managerial activities. However, these investors and other agents interested in sustainable investment need updated and robust information to support their decision making. We analyzed the performance of several Dow Jones Sustainability Indices (DJSIs) and compared them with their respective market benchmarks from 2013 to 2018. The indices comprise the following regions and countries: the world, the Asia‐Pacific, Europe, emerging markets and the US. The analysis was conducted based on both classic and modern portfolio metrics. The results suggest that sustainable investment performance is still heterogeneous worldwide, but there is a promising opportunity for investors to obtain superior risk‐adjusted returns in certain regions while incorporating sustainable investment practices. The findings are of utmost importance to financial market practitioners, business managers, academics and other stakeholders interested in promoting investments, corporate practices and scientific knowledge to achieve the Sustainable Development Goals (SDGs).
YAP1 promotes multidrug resistance of small cell lung cancer by CD74‐related signaling pathways
Authors: Song Yongchun , Sun Yanqin , Lei Yingying , Yang Kui , Tang Ruixiang 
Published: November 2019

AbstractBackgroundOur previous research found that YAP1 may have a role in multidrug resistance (MDR) in small cell lung cancer (SCLC). However, its underlying mechanism is unknown.MethodsIn this study, we investigated the expression of YAP1 using immunohistochemical staining and assessed the relationship between the expression of YAP1 and overall survival in patients with SCLC. We established H69 stable cell lines that overexpressed constitutively active YAP1 and H446 stable cell lines that dominate negative YAP1. We conducted CCK‐8, flow cytometric analysis, and in vivo chemosensitivity experiments to evaluate the function of YAP1 in drug sensitivity apoptosis in vitro and in vivo.ResultsThe results indicated that patients with high YAP1 expression have shorter survival rates and more advanced disease stage than those with low YAP1 expression. YAP1 may induce MDR by inhibiting the apoptosis of SCLC. YAP1 induced MDR when YAP1 was hyperactivated, and drug sensitivity increased when YAP1 was inhibited in vitro and in vivo. CD74 was significantly correlated with YAP1 in SCLC samples. Inhibition of CD74 using ISO‐1 increased drug sensitivity significantly.ConclusionsThe expression of YAP1 is significantly correlated with overall survival and disease stage in patients with SCLC. YAP1 may play an important role in these patients. We were the first to report that YAP1 can induce MDR in SCLC in vitro and in vivo. CD74 may be involved in YAP1‐induced MDR.