bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Structure of Tweets About Vaccine Safety Between Health Organizations, Experts, and the Public: Analyzing Risk Communication Conversations
Authors: Manheim David , Gesser-Edelsburg Anat 
Published: October 2020

AbstractObjective:This article considers how health education organizations in the World Health Organization's 9 Vaccine Safety Network (VSN) use Twitter to communicate about vaccines with the public, and whether they answer questions and engage in conversations. Almost no research in public health, to our knowledge, has explored conversational structure on social media among posts sent by different accounts.Methods:Starting with 1,017,176 tweets by relevant users, we constructed 2 corpuses of multi-tweet conversations. The first was 1,814 conversations that included VSN members directly, whereas the second was 2,283 conversations mentioning vaccines or vaccine denialism. The tweets and user metadata were then analyzed using an adaptation of rhetorical structure theory.Results:In the studied data, VSN members tweeted 12,677 times within conversations, compared to their 37,587 lone tweets. Their conversations were shorter than those in the comparison corpus (P < 0.0001), and they were involved in fewer multilogues (P < 0.0001).Conclusion:While there is diversity among organizations, most were tied to the pre-social media broadcast model. In the future, they should try to converse more, rather than tweet more, and embrace best practices in risk communication.
OnTemnocephala axenosMonticelli, 1898 (Platyhelminthes, Temnocephalida): taxonomic status and designation of a neotype
Authors: Seixas S.A. , Amato S.B. , Amato J.F.R. , Daudt L.C.C. , Damborenea C. 
Published: October 2020

AbstractTemnocephala axenosMonticelli, 1898 was described based on specimens from an unidentified host collected in Blumenau, Santa Catarina, Brazil. Information about type locality was imprecise and the host was later identified asAegla laevis(Latreille, 1818). However, it is known thatA. laevisis not present on the eastern side of the Andes. Also, only histological preparations from one specimen studied by Monticelli are currently available in the Museum für Naturkunde Berlin, but it showed none of the taxonomic characters needed for the characterization of the species. Although the updated description of the species based on Uruguayan specimens, neither the author nor the several previous studies about the species showed a search for the type material, a resolution for the misidentification of the type host or the imprecise type locality due to the subsequent geographical division of the municipality cited in the description. The Uruguayan specimens were not even geographically close to the type locality and a neotype was not designed to validate the species’ taxonomic status again. Specimens from Santa Catarina and Paraná States, Brazil, were studied, as well as restudied Argentinean specimens. The new data were compared with the update description of the species. The historical background and the discussion about geographical origins and hosts of the species, as well as a designation of a neotype, allow comparative material of the type locality and type host to exist, eliminating doubts about the identification ofT. axenos.
Correlates of sugar-sweetened beverage intake among low-income women during the first 1000 days
Authors: Cheng Erika R , Batista Elizabeth , Chen Ling , Nichols Kelsey , Park Sohyun , Charles Nalini , Woo Baidal Jennifer 
Published: October 2020

AbstractObjective:To describe prenatal and postpartum consumption of water, cows’ milk, 100 % juice and sugar-sweetened beverages (SSB) among women enrolled in the Special Supplemental Nutrition Program for Women, Infants and Children (WIC) programme in New York City (NYC) and to identify correlates of SSB intake in this population.Design:Cross-sectional data were collected from structured questionnaires that included validated beverage frequency questionnaires with the assistance of container samples. The association of maternal and household factors and non-SSB consumption with habitual daily energetic (kJ (kcal)) intake from SSB was assessed by using multivariable median regression.Setting:WIC programme in NYC, NY. Data were collected in 2017.Participants:388 pregnant or postpartum women (infant aged <2 years) from the NYC First 1000 Days Study.Results:Median age was 28 years (interquartile range (IQR) 24–34); 94·1 % were Hispanic/Latina, and 31·4 % were pregnant. Overall, 87·7 % of pregnant and 89·1% of postpartum women consumed SSB ≥ once weekly, contributing to a median daily energetic intake of 410 kJ (98 kcal) (IQR (113–904 kJ) 27–216) and 464 kJ (111 kcal) (IQR (163–1013 kJ) 39–242), respectively. In adjusted analyses, only consumption of 100 % juice was associated with greater median energetic intake from SSB (adjusted β for each additional ounce = 13; 95% CI 8, 31 (3·2; 95 % CI 2·0, 7·3).Conclusions:Among pregnant and postpartum women in WIC-enrolled families, interventions to reduce SSB consumption should include reduction of 100 % juice consumption as a co-target of the intervention.
Global Biogeochemical Cycle of Fluorine
Authors: Schlesinger William H. , Klein Emily M. , Vengosh Avner 
Published: October 2020

AbstractThis review provides a synthesis of what is currently known about the natural and anthropogenic fluxes of fluorine on Earth, offering context for an evaluation of the growing environmental impact of human‐induced F mobilization and use. The largest flux of F at the Earth's surface derives from the mobilization of F during chemical (2.2 Tg F/yr (where 1 Tg = 1012 g) and mechanical (7 Tg F/yr) weathering of rocks. Humans supplement these fluxes by mining fluorospar and apatite ores to make a variety of industrial chemicals and fertilizers, mobilizing 2.9 and 7.6 Tg F/yr, respectively. Other large anthropogenic fluxes derive from the manufacture of bricks (1.8 Tg F/yr) and extraction of groundwater (0.9 to 1.7 Tg F/yr). Rivers deliver ~3.6 Tg/yr of dissolved fluoride to the oceans, where the mean residence time of dissolved F in seawater is ~500,000 yr. F is removed from the oceans by the deposition of terrigenous (4.3 Tg F/yr) and authigenic sediments (1.24 Tg F/yr), and approximately 10 Tg F/yr is removed from the surface of the Earth by subduction of the oceanic lithosphere. Humans have increased the flux of F to the atmosphere and in rivers by more than a factor of 2, with the largest impacts stemming from the use of phosphorus fertilizers, the production of brick, and extraction of groundwater. Despite their well‐documented toxicity, perfluoroalkyl substances make only a small contribution to F emitted to the atmosphere and natural waters.
An Inclination Shallowing‐Corrected Early Triassic Paleomagnetic Pole for the North China Craton: Implication for the Mesozoic Geography of Proto‐Asia
Authors: Zhao Pan , Appel Erwin , Xu Bei 
Published: October 2020

AbstractThe Early Mesozoic is a key period for the formation of the Asia continent. In this study, in order to reconstruct the Early Mesozoic paleogeography of Proto‐Asia, we carried out a paleomagnetic study on the Early Triassic strata from the Chengde Basin in the northeastern North China Craton (NCC). Rock magnetic investigations reveal magnetite and hematite as the main magnetic carriers. Characteristic remanent magnetization directions from 21 sites show normal and reversed polarities and site‐mean directions pass fold tests. After inclination shallowing (IS) correction with the elongation/inclination method, a mean direction is calculated at Ds/Is = 322.5°/39.5° (ks = 32.6, α95s = 5.7°) in stratigraphic coordinates. The corresponding Early Triassic paleomagnetic pole (λ/φ = 53.5°N/9.5°E, A95 = 5.3°) overlaps with coeval poles that have also been corrected for IS reported from the western part of the NCC. This result indicates that there is no relative movement between the eastern and western parts of the NCC. With all IS‐corrected paleomagnetic poles, a mean Early Triassic pole is recalculated for the NCC at λ/φ = 59.4°N/13.4°E (A95 = 4.7°). We use this pole to reconstruct the paleogeography of Proto‐Asia and confirm previous studies that did not use IS corrections, in which two “triangle bay‐shaped” oceans, the Paleo‐Tethys Ocean and the Mongol‐Okhotsk Ocean, existed along the southern and northern margins of the unified NCC‐Mongolian Block (MOB). Clockwise rotations of the South China Craton and Siberia Craton relative to the NCC‐MOB during the Early Mesozoic led to the final closure of both oceans.
Baseline immunity and impact of chemotherapy on immune microenvironment in cervical cancer
Authors: Zhang Yi , Yu Minhua , Jing Ying , Cheng Jiejun , Zhang Caiyan , Cheng Lin , Lu Haijiao , Cai Mei-Chun , Wu Jie , Wang Wenjing , Lou Weihua , Qiu Lihua , Tan Li , Lu Huaiwu , Yin Xia , Zhuang Guanglei , Di Wen 
Published: October 2020

AbstractBackgroundWe aimed to comprehensively evaluate the immunologic landscape at baseline and upon chemotherapy in cervical cancer. The information should aid ongoing clinical investigations of checkpoint blockade immunotherapies in this disease setting.MethodsA series of 109 cervical carcinoma patients was retrospectively assayed before and after neoadjuvant chemotherapy. Tumour-infiltrating immune markers (CD3, CD4, CD8, CD20, CD56, CD68, PD-1, PD-L1) were assessed by immunohistochemistry. RNA sequencing analysis was performed on matched pre- and post-treatment fresh-frozen tissues.ResultsAt diagnosis, diverse immune cell types including CD20+ B cells, CD3+ T cells, CD56+ natural killer (NK) cells, and CD68+ macrophages were detected in different proportions of cervical carcinoma. Unsupervised hierarchical clustering evidently showed that CD4+ and CD8+ T cell abundance correlated with PD-L1 expression. Based on the immune infiltration patterns, the patients could be stratified into four groups with prognostic relevance, namely, ‘immuno-active’, ‘immuno-medial’, ‘immuno-NK’, and ‘immuno-deficient’. Neoadjuvant chemotherapy was associated with increased CD4, CD8, CD20, and CD56 signals, most prominently in good responders. Transcriptomic data corroborated the improved anticancer immunity and identified immunosuppressive CD200 upregulation following chemotherapeutic intervention.ConclusionsA subset of cervical cancer harbours active immune microenvironment, and chemotherapy treatment may further exert locoregional immunostimulation. Immune checkpoint inhibitors as combination or maintenance therapies warrant future exploration in clinic.
LncRNA MNX1-AS1 promotes progression of intrahepatic cholangiocarcinoma through the MNX1/Hippo axis
Authors: Li Fengwei , Chen Qinjunjie , Xue Hui , Zhang Lei , Wang Kui , Shen Feng 
Published: October 2020

AbstractLong non-coding RNAs (lncRNAs) have extremely complex roles in the progression of intrahepatic cholangiocarcinoma (ICC) and remain to be elucidated. By cytological and animal model experiments, this study demonstrated that the expression of lncRNA MNX1-AS1 was remarkably elevated in ICC cell lines and tissues, and was highly and positively correlated with motor neuron and pancreas homeobox protein 1 (MNX1) expression. MNX1-AS1 significantly facilitated the proliferation, migration, invasion, and angiogenesis in ICC cells in vitro, and remarkably promoted tumor growth and metastasis in vivo. Further study revealed that MNX1-AS1 promoted the expression of MNX1 via recruiting transcription factors c-Myc and myc-associated zinc finger protein (MAZ). Furthermore, MNX1 upregulated the expression of Ajuba protein via binding to its promoter region, and subsequently, Ajuba protein suppressed the Hippo signaling pathway. Taken together, our results uncovered that MNX1-AS1 can facilitate ICC progression via MNX1-AS1/c-Myc and MAZ/MNX1/Ajuba/Hippo pathway, suggesting that MNX1-AS1 may be able to serve as a potential target for ICC treatment.
ClC-3/SGK1 regulatory axis enhances the olaparib-induced antitumor effect in human stomach adenocarcinoma
Authors: Gu Zhuoyu , Wang Liping , Yao Xiaohan , Long Qian , Lee Kaping , Li Jieyao , Yue Dongli , Yang Shuangning , Liu Yanfen , Li Na , Li Yixin 
Published: October 2020

AbstractCurrently, only a few available targeted drugs are considered to be effective in stomach adenocarcinoma (STAD) treatment. The PARP inhibitor olaparib is a molecularly targeted drug that continues to be investigated in BRCA-mutated tumors. However, in tumors without BRCA gene mutations, particularly in STAD, the effect and molecular mechanism of olaparib are unclear, which largely restricts the use of olaparib in STAD treatment. In this study, the in vitro results showed that olaparib specifically inhibited cell growth and migration, exerting antitumor effect in STAD cell lines. In addition, a ClC-3/SGK1 regulatory axis was identified and validated in STAD cells. We then found that the down-regulation of ClC-3/SGK1 axis attenuated olaparib-induced cell growth and migration inhibition. On the contrary, the up-regulation of ClC-3/SGK1 axis enhanced olaparib-induced cell growth and migration inhibition, and the enhancement effect could be attenuated by SGK1 knockdown. Consistently, the whole-cell recorded chloride current activated by olaparib presented the same variation trend. Next, the clinical data showed that ClC-3 and SGK1 were highly expressed in human STAD tissues and positively correlated (r = 0.276,P = 0.009). Furthermore, high protein expression of both ClC-3 (P = 0.030) and SGK1 (P = 0.006) was associated with poor survival rate in STAD patients, and positive correlations between ClC-3/SGK1 and their downstream molecules in STAD tissues were demonstrated via the GEPIA datasets. Finally, our results suggested that olaparib inhibited the PI3K/AKT pathway in STAD cells, and up-regulation of ClC-3/SGK1 axis enhanced olaparib-induced PI3K/AKT pathway inhibition. The animal experiments indicated that olaparib also exerted antitumor effect in vivo. Altogether, our findings illustrate that olaparib exerts antitumor effect in human STAD, and ClC-3/SGK1 regulatory axis enhances the olaparib-induced antitumor effect. Up-regulation of the ClC-3/SGK1 axis may provide promising therapeutic potential for the clinical application of olaparib in STAD treatment.
BCL11B suppresses tumor progression and stem cell traits in hepatocellular carcinoma by restoring p53 signaling activity
Authors: Yang Wen-Jing , Sun Yun-Fan , Jin An-Li , Lv Li-Hua , Zhu Jie , Wang Bei-Li , Zhou Yan , Zhang Chun-Yan , Wang Hao , Hu Bo , Wang Peng-Xiang , Te Liu , Pan Bai-Shen , Zhou Jian , Fan Jia , Yang Xin-Rong , Guo Wei 
Published: October 2020

AbstractAccumulating evidence indicates that hepatocellular carcinoma (HCC) tumorigenesis, recurrence, metastasis, and therapeutic resistance are strongly associated with liver cancer stem cells (CSCs), a rare subpopulation of highly tumorigenic cells with self-renewal capacity and differentiation potential. Previous studies identified B cell leukemia/lymphoma-11b (BCL11B) as a novel tumor suppressor with impressive capacity to restrain CSC traits. However, the implications of BCL11B in HCC remain unclear. In this study, we found that low BCL11B expression was an independent indicator for shorter overall survival (OS) and time to recurrence (TTR) for HCC patients with surgical resection. In vitro and in vivo experiments confirmed BCL11B as a tumor suppressor in HCC with inhibitory effects on proliferation, cell cycle progression, apoptosis, and mobility. Furthermore, BCL11B could suppress CSC traits, as evidenced by dramatically decreased tumor spheroid formation, self-renewal potential and drug resistance. A Cignal Finder Array and dual-luciferase activity reporter assays revealed that BCL11B could activate the transcription of P73 via an E2F1-dependent manner. Thus, we concluded that BCL11B is a strong suppressor of retaining CSC traits in HCC. Ectopic expression of BCL11B might be a promising strategy for anti-HCC treatment with the potential to cure HBV-related HCC regardless of P53 mutation status.
Cancer-testis antigens, semenogelins 1 and 2, exhibit different anti-proliferative effects on human lung adenocarcinoma cells
Authors: Shuvalov Oleg , Kizenko Alyona , Petukhov Alexey , Aksenov Nickolai , Fedorova Olga , Vorobev Mikhail , Daks Alexandra , Barlev Nickolai 
Published: October 2020

AbstractСancer-testis antigens (CTAs) comprise proteins which are aberrantly expressed in various malignancies, yet under normal situation are restricted to only germ cells. Semenogelins 1 and 2 (SEMG1 and 2, respectively) belong to the family of non-X-linked (autosomal) cancer-testis antigens. They are the major protein ingredients of human semen and share 78% of similarity between them on the gene level. SEMG1/2 gene products regulate the motility and fertility of sperm, as well as provide sperm the antibacterial defense. Besides, SEMG1 and SEMG2 were detected in various malignancies including small cell lung cancer (SCLC). However, the biological role of both SEMG1 and 2 proteins in tumorigenesis has not been fully understood. We demonstrate here that SEMG1 and SEMG2 (SEMGs) exhibit different patterns of expression and sub-cellular localization in non-small cell lung cancer (NSCLC) cell lines. To elucidate the biological properties of SEMGs in NSCLC, we established H1299 cell lines that were stably transduced with either SEMGs-overexpressing or knockdown vectors, respectively. Using fluorescence-based dihydroethidium (DHE) assay we showed that both SEMGs augmented the production of reactive oxygen species (ROS) up to 2 times. Moreover, SEMGs (especially SEMG1) strongly increased the number of Annexin V–positive apoptotic cells manifesting an increased sensitivity to genotoxic drugs including doxorubicin, etoposide, and cisplatin. Taken our results together, SEMGs may arguably play a positive role in tumorigenesis by sensitizing NSCLCs to genotoxic therapy.
High-temperature ferromagnetic semiconductor with a field-tunable green fluorescent effect
Authors: Zhou Bowen , Zhao Qing , Liu Zhehong , Shen Xudong , Ye Xubin , Shi Jiangjian , Liao Zhiyu , Wang Weipeng , Hu Zhiwei , Lin Hong-Ji , Chen Chien-Te , Bian Yuecheng , Sheng Zhigao , Yu Richeng , Qiu Xianggang , Meng Qingbo , Li Zhi , Long Youwen 
Published: October 2020

AbstractFerromagnetic semiconductors with luminescent effects provide a unique platform for studying magneto-electric-optical multifunctional devices. However, little is known about such materials with spin ordering well above room temperature. By using a unique high-pressure annealing method, a Cr and Fe disordered perovskite oxide SrCr0.5Fe0.5O2.875 (SCFO) with a simple cubic structure was prepared. Magnetic measurements demonstrated the ferromagnetic behavior with a spin ordering temperature as high as 600 K. In contrast to metallic SrCrO3 and SrFeO3, SCFO, with a moderate oxygen deficiency, is a direct bandgap semiconductor with an energy gap of 2.28 eV, which is within the visible light region. As a consequence, SCFO displays a green fluorescent effect arising from the d–p bonding and anti-bonding states. Moreover, the photoluminescence intensity can be tuned by a magnetic field. This work opens up a new avenue for research on room-temperature multifunctional materials with coupled magnetic, electrical, and optical performance.
Natural antisense transcripts of MIR398 genes suppress microR398 processing and attenuate plant thermotolerance
Authors: Li Yajie , Li Xiaorong , Yang Jun , He Yuke 
Published: October 2020

AbstractMicroRNAs (miRNAs) and natural antisense transcripts (NATs) control many biological processes and have been broadly applied for genetic manipulation of eukaryotic gene expression. Still unclear, however, are whether and how NATs regulate miRNA production. Here, we report that the cis-NATs of MIR398 genes repress the processing of their pri-miRNAs. Through genome-wide analysis of RNA sequencing data, we identify cis-NATs of MIRNA genes in Arabidopsis and Brassica. In Arabidopsis, MIR398b and MIR398c are coexpressed in vascular tissues with their antisense genes NAT398b and NAT398c, respectively. Knock down of NAT398b and NAT398c promotes miR398 processing, resulting in stronger plant thermotolerance owing to silencing of miR398-targeted genes; in contrast, their overexpression activates NAT398b and NAT398c, causing poorer thermotolerance due to the upregulation of miR398-targeted genes. Unexpectedly, overexpression of MIR398b and MIR398c activates NAT398b and NAT398c. Taken together, these results suggest that NAT398b/c repress miR398 biogenesis and attenuate plant thermotolerance via a regulatory loop.
Mode of induction of platelet-derived extracellular vesicles is a critical determinant of their phenotype and function
Authors: Ferreira P. M. , Bozbas E. , Tannetta S. D. , Alroqaiba N. , Zhou R. , Crawley J. T. B. , Gibbins J. M. , Jones C. I. , Ahnström J. , Yaqoob P. 
Published: October 2020

AbstractPlatelet-derived extracellular vesicles (PDEVs) are the most abundant amongst all types of EVs in the circulation. However, the mechanisms leading to PDEVs release, their role in coagulation and phenotypic composition are poorly understood. PDEVs from washed platelets were generated using different stimuli and were characterised using nanoparticle tracking analysis. Procoagulant properties were evaluated by fluorescence flow cytometry and calibrated automated thrombography. EVs from plasma were isolated and concentrated using a novel protocol involving a combination of size exclusion chromatography and differential centrifugation, which produces pure and concentrated EVs. Agonist stimulation enhanced PDEV release, but did not alter the average size of EVs compared to those produced by unstimulated platelets. Agonist stimulation led to lower negatively-charged phospholipid externalization in PDEVs, which was reflected in the lower procoagulant activity compared to those generated without agonist stimulation. Circulating EVs did not have externalized negatively-charged phospholipids. None of the 4 types of EVs presented tissue factor. The mechanism by which PDEV formation is induced is a critical determinant of its phenotype and function. Importantly, we have developed methods to obtain clean, concentrated and functional EVs derived from platelet-free plasma and washed platelets, which can be used to provide novel insight into their biological functions.