bluuebunny/crossref_metadata_2025_split huggingface dataset:
Left Hepatic Vein Preferential Approach Based on Anatomy Is Safe and Feasible for Laparoscopic Living Donor Left Lateral Sectionectomy
Authors: Lu Lu , Wang Zheng‐Xin , Zhu Wen‐Wei , Shen Cong‐Huan , Tao Yi‐Feng , Ma Zhen‐Yu , Zhang Quan‐Bao , Li Rui‐Dong , Jia Hu‐Liang , Qin Lun‐Xiu , Chen Jin‐Hong 
Published: May 2020

We assess the safety and feasibility of the left hepatic vein preferential approach (LHVPA) based on left hepatic vein (LHV) anatomy for living donor laparoscopic left lateral sectionectomy (LLLS). Data from 50 donors who underwent LLLS in Huashan Hospital from October 2016 to November 2019 were analyzed retrospectively. On the basis of the classification of the LHV anatomy, the vein was defined as the direct import type, upper branch type, or indirect import type. A subgroup analysis was performed to compare the outcomes between the LHVPA and non‐LHVPA groups. All 50 patients underwent pure LLLS. The mean operative duration was 157.5 ± 29.7 minutes. The intraoperative blood loss was 160.4 ± 97.5 mL. No complications more severe than grade 3 occurred. LHVPA was applied in 13 patients, whereas non‐LHVPA was applied in 10 patients with the direct import type and upper branch type anatomy. The operative duration was shorter in the LHVPA group than the non‐LHVPA group (142.7 ± 22.0 versus 173.0 ± 22.8 minutes; P = 0.01). Intraoperative blood loss was reduced in the LHVPA group compared with the non‐LHVPA group (116.2 ± 45.6 versus 170.0 ± 63.3 mL; P = 0.02). The length of the LHV reserved extrahepatically in the LHVPA group was longer than in the non‐LHVPA group (4.3 ± 0.2 versus 3.3 ± 0.3 mm; P = 0.01). Fewer reconstructions of the LHV in the direct import type anatomy were required for the LHVPA group than for the non‐LHVPA group (0/8 versus 4/6). LHVPA based on the LHV anatomy is recommended in LLLS because it can further increase the safety and the efficiency of surgery for suitable donors.
The optimization system for preparation of TG1 competent cells and electrotransformation
Authors: Chai Dafei , Wang Gang , Fang Lin , Li Huizhong , Liu Shanshan , Zhu Haiying , Zheng Junnian 
Published: May 2020

AbstractAn efficient electrotransformation system that includes electrocompetent cells is a critical component for the success of large‐scale gene transduction and replication. The conditions of TG1 competent cell preparation and optimal electrotransformation were evaluated by investigating different parameters. Certain parameters for preparation of TG1 competent cells (≥8 × 1010 colony forming units (cfu)/μg DNA) include optimum culture time of monoclonal bacteria (8–10 hr), amplification growth concentration (approximately OD600 = 0.45), and culture volume (400 ml in 2 L conical flask). With increased storage of competent cells at −80°C, electrotransformation efficiency gradually decreased, but it remains greater than ≥ 1010 cfu/μg DNA 3 months later. Moreover, the recovery time of electrotransformation also influenced electrotransformation efficiency (1.5–2 hr for optimization). The optimized transformation efficiency of TG1 (≥8 × 1010 cfu/μg DNA) was observed under suitable electric voltage (2.5 kV), electric intensity (15 kV/cm), and electric time (3.5 ms) of electricity for plasmid transformation. Optimized DNA amount (0.01–100 ng) dissolved in water led to the high efficiency of plasmid transformation (≥8 × 1010 cfu/μg DNA), but had low efficiency when dissolved in T4 ligation buffer (≤3 × 1010 cfu/μg DNA). These results indicated that an optimized TG1 transformation system is useful for high electrotransformation efficiency under general laboratory conditions. The optimized TG1 transformation system might facilitate large‐scale gene transduction for phage display library construction.
Functional Motor Disorders Mimicking Symptoms Upon Resolution of Cerebrovascular Disease
Authors: Paio Fabio , Antelmi Elena , Conti Enrico , Di Vico Ilaria , Tinazzi Michele 
Published: May 2020

ABSTRACTBackgroundFunctional motor disorders encompass a variety of manifestations characterized by abnormal movements that are clinically incongruent with those known to be caused by neurological diseases.CasesWe report 2 cases in which functional motor disorders developed after complete recovery of motor symptoms originating from underlying vascular brain lesions. The first case describes a young woman who developed a motor and sensory hemisyndrome after surgical removal of a postrolandic cavernoma. The second describes a 16‐year‐old girl who presented with a mixed component tremor after ventricular derivation and endovascular embolization for rupture of a brainstem arteriovenous malformation.ConclusionMotor symptoms and signs attributed to an underlying lesion may trigger “pure” functional motor disorders. In such cases, the differential diagnosis may be even more challenging. However, diagnosis of a functional rather than a defined structural disorder can be achieved by a “positive diagnostic process,” considering the findings of internal inconsistency and incongruity.
Magnetic Resonance Imaging Biomarkers Distinguish Normal Pressure Hydrocephalus From Progressive Supranuclear Palsy
Authors: Quattrone Andrea , Sarica Alessia , La Torre Domenico , Morelli Maurizio , Vescio Basilio , Nigro Salvatore , Barbagallo Gaetano , Nisticò Rita , Salsone Maria , Arcuri Pier Paolo , Novellino Fabiana , Bianco Maria Giovanna , Arabia Gennarina , Cascini Giuseppe , Quattrone Aldo 
Published: May 2020

ABSTRACTBackgroundIdiopathic normal pressure hydrocephalus and PSP share several clinical and radiological features, making differential diagnosis, at times, challenging.ObjectivesTo differentiate idiopathic normal pressure hydrocephalus from PSP using MR volumetric and linear measurements.MethodsTwenty‐seven idiopathic normal pressure hydrocephalus patients, 103 probable PSP patients, and 43 control subjects were consecutively enrolled. Automated ventricular volumetry was performed using Freesurfer 6 on MR T1‐weighted images. Linear measurements, such as callosal angle and a new measure, termed MR Hydrocephalic Index, were calculated on MR T1‐weighted images. Receiver operating characteristic analyses were used for differentiating between patient groups. Generalizability and reproducibility of the results were validated, dividing each participant group in two cohorts used as training and testing subsets.ResultsVentricular volumes and linear measurements (callosal angle and Magnetic Resonance Hydrocephalic Index) revealed greater ventricular enlargement in patients with idiopathic normal pressure hydrocephalus than in PSP patients and controls. PSP patients had ventricular volume larger than controls. Automated ventricular volumetry and Magnetic Resonance Hydrocephalic Index were the most accurate measures (98.5%) in differentiating patients with idiopathic normal pressure hydrocephalus from PSP patients, whereas callosal angle misclassified several PSP patients and showed low positive predictive value (70.0%) in differentiating between these two diseases. All measurements accurately differentiated idiopathic normal pressure hydrocephalus patients from controls. Accuracy values obtained in the training set (automated ventricular volumetry, 98.4%; Magnetic Resonance Hydrocephalic Index, 98.4%; callosal angle, 87.5%) were confirmed in the testing set.ConclusionsOur study demonstrates that AVV and Magnetic Resonance Hydrocephalic Index were the most accurate measures for differentiation between idiopathic normal pressure hydrocephalus and PSP patients. Magnetic Resonance Hydrocephalic Index is easy to measure and can be used in clinical practice to prevent misdiagnosis and ineffective shunt procedures in idiopathic normal pressure hydrocephalus mimics. © 2020 International Parkinson and Movement Disorder Society
A novel prognostic model based on multi‐omics features predicts the prognosis of colon cancer patients
Authors: Yang Haojie , Jin Wei , Liu Hua , Wang Xiaoxue , Wu Jiong , Gan Dan , Cui Can , Han Yilin , Han Changpeng , Wang Zhenyi 
Published: May 2020

AbstractBackgroundAs a common malignant tumor in the colon, colon cancer (CC) has high incidence and recurrence rates. This study is designed to build a prognostic model for CC.MethodsThe gene expression dataset, microRNA‐seq dataset, copy number variation (CNV) dataset, DNA methylation dataset, and transcription factor (TF) dataset of CC were downloaded from UCSC Xena database. Using limma package, the differentially methylated genes (DMGs), and differentially expressed genes (DEGs) and miRNAs (DEMs) were identified. Based on random forest method, prognostic model for each omics dataset were constructed. After the omics features related to prognosis were selected using logrank test, the prognostic model based on multi‐omics features was built. Finally, the clinical phenotypes correlated with prognosis were screened using Kaplan–Meier survival analysis, and the nomogram model was established.ResultsThere were 1625 DEGs, 268 DEMs, and 386 DMGs between the tumor and normal samples. A total of 105, 29, 159, five, and six genes/sites significantly correlated with prognosis were identified in the gene expression dataset (GABRD), miRNA‐seq dataset (miR‐1271), CNV dataset (RN7SKP247), DNA methylation dataset (cg09170112 methylation site [located in SFSWAP]), and TF dataset (SIX5), respectively. The prognostic model based on multi‐omics features was more effective than those based on single omics dataset. The number of lymph nodes, pathologic_M stage, and pathologic_T stage were the clinical phenotypes correlated with prognosis, based on which the nomogram model was constructed.ConclusionThe prognostic model based on multi‐omics features and the nomogram model might be valuable for the prognostic prediction of CC.
Roles of HOTAIR in lung cancer susceptibility and prognosis
Authors: Ren Meng‐Meng , Xu Sen , Wei Yu‐Bo , Yang Juan‐Juan , Yang Ya‐Nan , Sun Shan‐Shan , Li You‐Jie , Wang Ping‐Yu , Xie Shu‐Yang 
Published: May 2020

AbstractBackgroundLong noncoding (lncRNA) single‐nucleotide polymorphisms (SNPs) are associated with the susceptibility to the development of various malignant tumors. The aim of this study was to investigate the roles of HOX transcript antisense intergenic RNA (HOTAIR) and its SNPs in lung cancer.MethodsInitially, the expression of HOTAIR in different tumors was investigated using the online Gene Expression Profiling Interactive Analysis (GEPIA) resource. Three SNPs (rs920778, rs1899663, and rs4759314) of HOTAIR were identified using the MassArray system. Following this, the relationship between these SNPs and susceptibility to lung cancer was investigated.ResultsExpression of HOTAIR was found to increase in a variety of cancers, including nonsmall cell lung cancer (NSCLC). We found that the genotypes of these SNPs (rs920778, rs1899663, and rs4759314) were not significantly associated with lung cancer type, family history, lymph node metastasis, or lung cancer stage. In gender stratification, the results of rs920778 genotypes showed that, compared to genotype AA, the AG (OR = 0.344, 95% CI: 0.133–0.893, p = .028) and AG + GG (OR = 0.378, 95% CI: 0.153–0.932, p = .035) genotypes of rs920778 are protective factors against NSCLC in females. In smoking stratification, compared with AA of rs920778, the genotype AG + GG (OR = 0.507, 95% CI: 0.263–0.975, p = .042) was a protective factor against NSCLC in nonsmoking people. No statistical differences were observed in the classifications of rs1899663 and rs4759314 genotypes. Linkage disequilibrium analysis revealed a high linkage disequilibrium between the rs920778 and rs1899663 (D′ = 0.99, r2 = .74), rs920778 and rs4759314 (D′ = 0.85, r2 = .13), and rs1899663 and rs4759314 (D′ = 0.79, r2 = .00).ConclusionOur study demonstrated that HOTAIR expression increased in NSCLC, and that the genotypes of rs920778 could be useful in the diagnosis and prognosis of lung cancer.
Comparison of robust to standardized CT radiomics models to predict overall survival for non‐small cell lung cancer patients
Authors: Vuong Diem , Bogowicz Marta , Denzler Sarah , Oliveira Carol , Foerster Robert , Amstutz Florian , Gabryś Hubert S. , Unkelbach Jan , Hillinger Sven , Thierstein Sandra , Xyrafas Alexandros , Peters Solange , Pless Miklos , Guckenberger Matthias , Tanadini‐Lang Stephanie 
Published: May 2020

BackgroundRadiomics is a promising tool for the identification of new prognostic biomarkers. Radiomic features can be affected by different scanning protocols, often present in retrospective and prospective clinical data. We compared a computed tomography (CT) radiomics model based on a large but highly heterogeneous multicentric image dataset with robust feature pre‐selection to a model based on a smaller but standardized image dataset without pre‐selection.Materials and methodsPrimary tumor radiomics was extracted from pre‐treatment CTs of IIIA/N2/IIIB NSCLC patients from a prospective Swiss multicentric randomized trial (npatient = 124, ninstitution = 14, SAKK 16/00) and a validation dataset (npatient = 31, ninstitution = 1). Four robustness studies investigating inter‐observer delineation variation, motion, convolution kernel, and contrast were conducted to identify robust features using an intraclass correlation coefficient threshold >0.9. Two 12‐months overall survival (OS) logistic regression models were trained: (a) on the entire multicentric heterogeneous dataset but with robust feature pre‐selection (MCR) and (b) on a smaller standardized subset using all features (STD). Both models were validated on the validation dataset acquired with similar reconstruction parameters as the STD dataset. The model performances were compared using the DeLong test.ResultsIn total, 113 stable features were identified (nshape = 8, nintensity = 0, ntexture = 7, nwavelet = 98). The convolution kernel had the strongest influence on the feature robustness (<20% stable features). The final models of MCR and STD consisted of one and two features respectively. Both features of the STD model were identified as non‐robust. MCR did not show performance significantly different from STD on the validation cohort (AUC [95%CI] = 0.72 [0.48–0.95] and 0.79 [0.63–0.95], p = 0.59).ConclusionPrognostic OS CT radiomics model for NSCLC based on a heterogeneous multicentric imaging dataset with robust feature pre‐selection performed equally well as a model on a standardized dataset.
Otolith and Scale-Based Growth Standards for Lotic Smallmouth Bass
Authors: Starks Trevor A. , Rodger Anthony W. 
Published: May 2020

Abstract
Comparisons of local population growth rates to species averages are valuable for natural resource managers because growth rates provide an integrated evaluation of abiotic and biotic factors that regulate growth in a fishery and may inform management decisions. Thus, species-specific standard growth models pave the way for fisheries scientists to efficiently complete comparisons, evaluate status, and determine the potential of a fishery for meeting goals and objectives. Growth standards have been published for numerous freshwater fishes; however, growth standards commonly neglect differences among populations of the same species that reside in different habitat types (e.g., lotic versus lentic habitats). Furthermore, studies may use data sets that are based on age estimates from various aging structures, even those with known biases, when creating standard growth models. Our study developed a standard growth model for lotic-dwelling Smallmouth Bass Micropterus dolomieu based on over 11,000 fish across their range in the United States. Due to differences in parameter estimates between standard growth equations that are generated with otolith- and scale-aged fish, separate standard growth models were created for each structure. Standard growth models were used to calculate the following growth standards: predicted age-specific standard length, percentile distributions of age-specific standard length, and estimates of the time required to reach specified size-classes for each aging structure. Thus, standard growth models and growth standards are presented for the two most common aging structures, which will facilitate comparisons of lotic Smallmouth Bass populations by providing equitable growth equations regardless of the aging structure that is used by researchers.
Exploring residential care aide experiences with oral malodour in long‐term care
Authors: Dhami Charanpreet Singh , Donnelly Leeann R. 
Published: May 2020

AbstractAimTo determine what experiences do residential care aides (RCAs) have with individuals living with oral malodour in a long‐term care (LTC) facility?DesignStudy investigation was done using an interpretive qualitative approach paired with a social constructivism interpretive framework.MethodsThe study was conducted in May of 2015 through face‐to‐face interviews with RCAs, which were recorded and transcribed verbatim, observations of RCAs in their work environment, as well as RCA personal logs of their daily experiences with odour during caregiving. Thereafter, data were analysed and coded for emerging themes.ResultsFive major themes were identified after grouping the codes: 1) attitudes and behaviours when caring for residents with malodour; 2)RCA knowledge of oral malodour; 3) level of job satisfaction among RCAs that care for residents with malodour; 4) culture and malodour; and 5) challenges of care giving compounded by malodour. These themes depict the overall experiences of study participants.
Registration of 11 diverse sorghum germplasm lines for grain and silage hybrid production
Authors: Horne David W. , Patil Nikhil Y. , Klein Robert R. , Miller Fred R. , Hoffmann Leo , Klein Patricia E. , Rooney William L. 
Published: May 2020

AbstractGenotypic variance is necessary for trait improvement because limited diversity can reduce genetic gains in crop improvement. To maintain genetic diversity, a wealth of germplasm exists in the USDA‐ARS sorghum [Sorghum bicolor (L.) Moench] collection, but most of the accessions are not adapted to temperate climates. To address this issue, the Reinstated Sorghum Conversion program (RSC, ∼2009–2014) converted 155 tropical accessions to combine height and early maturing F3 and BC1F3 families. Here, 11 germplasm lines Tx3429 to Tx3439 (Reg. no. GP‐889, PI 692640 to Reg. no. GP‐899, PI 692650) released by Texas A&M AgriLife Research in 2019 and derived from the RSC program are described. This germplasm was derived from F3, BC1F3, and BC1F4 RSC families that were selected based on testcross hybrid performance for either grain or silage production. Six lines are grain sorghum pollinators, one line is a seed parent, and four lines are silage pollinators. These lines combined agronomic productivity with greater genetic diversity as confirmed via genotyping‐by‐sequencing. These 11 parental germplasms are being released to provide new genetic diversity for forage and grain hybrid improvement programs.
FIELDimageR: An R package to analyze orthomosaic images from agricultural field trials
Authors: Matias Filipe Inácio , Caraza‐Harter Maria V. , Endelman Jeffrey B. 
Published: May 2020

AbstractRemote sensing is revolutionizing the phenotyping of agricultural field trials, but for many researchers, the extraction of plot‐level results is a bottleneck. We have developed the R package FIELDimageR as a user‐friendly tool to analyze orthomosaic images containing many plots. The basic workflow involves cropping and rotating the image, followed by the creation of a shapefile based on the experimental design. The package includes functions to calculate the number of plants per plot, canopy cover percentage, vegetation indices, and plant height. FIELDimageR is publicly available as a GitHub repository (https://github.com/filipematias23/FIELDimageR).
Ultrasound assessment of diaphragmatic function in type 1 spinal muscular atrophy
Authors: Buonsenso Danilo , Berti Beatrice , Palermo Concetta , Leone Daniela , Ferrantini Gloria , De Sanctis Roberto , Onesimo Roberta , Curatola Antonietta , Fanelli Lavinia , Forcina Nicola , Norcia Giulia , Carnicella Sara , Lucibello Simona , Mercuri Eugenio , Pane Marika 
Published: May 2020

AbstractObjectiveTo investigate ultrasound features of diaphragm motion and function in type 1 spinal muscular atrophy (SMA‐1) patients.DesignProspective study.PatientsThe study cohort included SMA‐1 children younger than 18‐year‐old. Control subjects included type 2 and type 3 SMA and other neuromuscular disorders younger than 18‐year‐old.MethodologyDiaphragm ultrasound evaluating diaphragmatic excursion, speed of diaphragmatic contraction, duration of the respiratory cycle, inspiratory/expiratory relationship, end‐inspiratory and ‐expiratory thickness, thickening fraction, and pattern of contractility. The interrater reliability for each variable was established by calculation of Cohen's k coefficient.ResultsTwenty‐three SMA‐1 patients and 12 controls were evaluated. Diaphragm ultrasound values were within normal ranges in all study cohort patients and no difference was found with controls. There was a gradient of diaphragm function with SMA 1.9 subgroup having the best and SMA 1.1 having the worst parameters, particularly in end‐inspiratory thickness and diaphragmatic excursion (P = .031 and P = .041, respectively). Seventy‐four percent of SMA‐1 patients had a dysmotility pattern of diaphragm contraction, mostly represented in SMA 1.9 subgroup (P = .001). This pattern was observed in 92.8% of children on noninvasive ventilation (NIV) for less than 16 hours/d of and in 20% patients with invasive ventilation or NIV for more than 16 hours/d (P = .027). The dysmotility pattern was never observed in the control group. The levels of interobserver agreement were high for “diaphragm irregularities,” “inspiratory/expiratory relationship,” and “diaphragm thickness,” and good for the other variables.ConclusionsUltrasound can be used to evaluate diaphragm function and contractility in SMA‐1 children, providing additional information to the clinical examination and functional respiratory tests, describing a characteristic contractility pattern in these patients. Longitudinal studies are needed to understand the impact of diaphragm dysmotility and other parameters on long‐term outcome in SMA‐1 patients.
Dexamethasone or prednisolone for asthma exacerbations in children: A cost‐effectiveness analysis
Authors: Rodriguez‐Martinez Carlos E. , Sossa‐Briceño Monica P. , Castro‐Rodriguez Jose A. 
Published: May 2020

AbstractObjectivesAlthough a short course (ie, 3 to 5 days) of orally administered prednisolone is a common and widely accepted practice among clinicians for administering systemic corticosteroids in pediatric acute asthma, oral dexamethasone for 1 to 2 days is an attractive alternative to prednisolone due to its better palatability and compliance. However, a cost‐effectiveness analysis regarding the use of dexamethasone compared to prednisolone is not sufficient, especially in lower‐ and middle‐income countries. The objective of this study was to analyze the cost‐effectiveness of prednisolone vs oral dexamethasone for treating pediatric asthma exacerbations.MethodsUsing a decision‐analysis model, we analyzed the cost‐effectiveness of prednisolone vs oral dexamethasone for treating acute pediatric asthma. Effectiveness parameters were derived from a systematic review of the published literature. Data for costs were acquired from hospital accounts and from an official national database, the national manual of drug prices in Colombia. The study was carried out from a Colombian third‐party payer perspective. The principal outcome of the model was the avoidance of hospitalization.ResultsThe base‐case analysis showed that compared to dexamethasone, administering prednisolone was associated with lower overall treatment costs (US$93.97 vs US$104.91 mean cost per patient) without a significant difference in the probability of hospitalization avoided (.9108 vs .9108).ConclusionsThe present study shows that in Colombia, a middle‐income country, compared with oral dexamethasone, the use of prednisolone for treating acute pediatric asthma is cost‐effective, yielding a similar probability of hospitalization at lesser overall costs.