bluuebunny/crossref_metadata_2025_split huggingface dataset:
New proofs on two recent inequalities for unitarily invariant norms
Authors: Yang Junjian , Lu Linzhang 
Published: May 2020

AbstractIn this short note, we provide alternative proofs for several recent results due to Audenaert (Oper. Matrices 9:475–479, 2015) and Zou (J. Math. Inequal. 10:1119–1122, 2016; Linear Algebra Appl. 552:154–162, 2019).
Clinical impact of endemic NDM-producing Klebsiella pneumoniae in intensive care units of the national referral hospital in Jakarta, Indonesia
Authors: Saharman Yulia Rosa , Karuniawati Anis , Sedono Rudyanto , Aditianingsih Dita , Goessens Wil H. F. , Klaassen Corné H. W. , Verbrugh Henri A. , Severin Juliëtte A. 
Published: May 2020

Abstract
Objective
A prospective observational study was performed to assess the epidemiology and clinical impact of carbapenem-non-susceptible Klebsiella pneumoniae (CNKP) in intensive care units (ICUs) of the national referral hospital in Jakarta, Indonesia.

Materials/methods
Adult patients consecutively hospitalized for > 48 h in two ICUs of the national referral hospital were included from April until October 2013 and from April until August 2014. K. pneumoniae from clinical cultures and standardized screening of rectum and throat on admission, discharge and weekly if hospitalized > 7 days were collected. Environmental niches and healthcare workers (HCWs) were also screened. Susceptibility was determined phenotypically and the presence of carbapenemase genes by PCR. Raman spectroscopy as well as multiple-locus variable number tandem repeat analysis (MLVA) were used for typing.

Results
Twenty-two out of 412 (5.3%) patients carried CNKP on admission and 37/390 (9.5%) acquired CNKP during ICU stay. The acquisition rate was 24.7/1000 patient-days at risk. One out of 31 (3.2%) environmental isolates was a CNKP. None of the HCWs carried CNKP. Acquisition of CNKP was associated with longer ICU stay (adjusted Hazard Ratio: 2.32 [CI99: 1.35–3.68]). ICU survival was lower among patients with CNKP compared to patients with carbapenem-susceptible K. pneumoniae (aHR 2.57, p = 0.005). Ninety-six of the 100 (96%) CNKP isolates carried a carbapenemase gene, predominantly blaNDM. Raman typing revealed three major clusters among 48 Raman types identified, whereas MLVA distinguished six major clusters among a total of 30 different genotypes.

Conclusions
NDM-producing CNKP are introduced into these ICUs and some strains expand clonally among patients and the environment, resulting in endemic CNKP. CNKP acquisition was associated with prolonged ICU stay and may affect ICU survival.

Trial registration
The study was registered at Netherlands Trial Register http://www.trialregister.nl. Candidate number: 23527, NTR number: NTR5541, NL number: NL5425 (https://www.trialregister.nl/trial/5424), Retrospectively registered: NTR: 22 December 2015.
The emerging roles of vacuolar-type ATPase-dependent Lysosomal acidification in neurodegenerative diseases
Authors: Song Qiaoyun , Meng Bo , Xu Haidong , Mao Zixu 
Published: May 2020

AbstractBackgroundLysosomes digest extracellular material from the endocytic pathway and intracellular material from the autophagic pathway. This process is performed by the resident hydrolytic enzymes activated by the highly acidic pH within the lysosomal lumen. Lysosome pH gradients are mainly maintained by the vacuolar (H+) ATPase (or V-ATPase), which pumps protons into lysosomal lumen by consuming ATP. Dysfunction of V-ATPase affects lysosomal acidification, which disrupts the clearance of substrates and leads to many disorders, including neurodegenerative diseases.Main bodyAs a large multi-subunit complex, the V-ATPase is composed of an integral membrane V0 domain involved in proton translocation and a peripheral V1 domain catalyzing ATP hydrolysis. The canonical functions of V-ATPase rely on its H+-pumping ability in multiple vesicle organelles to regulate endocytic traffic, protein processing and degradation, synaptic vesicle loading, and coupled transport. The other non-canonical effects of the V-ATPase that are not readily attributable to its proton-pumping activity include membrane fusion, pH sensing, amino-acid-induced activation of mTORC1, and scaffolding for protein-protein interaction. In response to various stimuli, V-ATPase complex can reversibly dissociate into V1 and V0 domains and thus close ATP-dependent proton transport. Dysregulation of pH and lysosomal dysfunction have been linked to many human diseases, including neurodegenerative disorders such as Alzheimer disease, Parkinson’s disease, amyotrophic lateral sclerosis as well as neurodegenerative lysosomal storage disorders.ConclusionV-ATPase complex is a universal proton pump and plays an important role in lysosome acidification in all types of cells. Since V-ATPase dysfunction contributes to the pathogenesis of multiple neurodegenerative diseases, further understanding the mechanisms that regulate the canonical and non-canonical functions of V-ATPase will reveal molecular details of disease process and help assess V-ATPase or molecules related to its regulation as therapeutic targets.
Identification of rare and common variants in BNIP3L: a schizophrenia susceptibility gene
Authors: Zhou Juan , Ma Chuanchuan , Wang Ke , Li Xiuli , Jian Xuemin , Zhang Han , Yuan Jianmin , Yin Jiajun , Chen Jianhua , Shi Yongyong 
Published: May 2020

Abstract
Background
Schizophrenia is a chronic and severe mental disorder, and it has been predicted to be highly polygenic. Common SNPs located in or near BNIP3L were found to be genome-wide significantly associated with schizophrenia in recent genome-wide association studies. The purpose of our study is to investigate potential causal variants in BNIP3L gene.

Results
We performed targeted sequencing for all exons and un-translated regions of BNIP3L gene among 1806 patients with schizophrenia and 998 healthy controls of Han Chinese origin. Three rare nonsynonymous mutations, BNIP3L (NM_004331): c.52A>G, c.167G>A and c.313A>T, were identified in schizophrenia cases, and two of them were newly reported. The frequencies of these rare nonsynonymous mutations were significantly different between schizophrenia cases and healthy controls. For the common variants, rs147389989 achieved significance in both allelic and genotypic distributions with schizophrenia. Rs1042992 and rs17310286 were significantly associated with schizophrenia in meta-analyses using PGC, CLOZUK, and our new datasets in this study.

Conclusions
Our findings provided further evidence that BNIP3L gene is a susceptibility gene of schizophrenia and revealed functional and potential causal mutations in BNIP3L. However, more functional validations are suggested to better understand the role of BNIP3L in the etiology of schizophrenia.
Association between low pH and unfavorable neurological outcome among out-of-hospital cardiac arrest patients treated by extracorporeal CPR: a prospective observational cohort study in Japan
Authors:   on behalf of the CRITICAL Study Group Investigators, Okada Yohei , Kiguchi Takeyuki , Irisawa Taro , Yoshiya Kazuhisa , Yamada Tomoki , Hayakawa Koichi , Noguchi Kazuo , Nishimura Tetsuro , Ishibe Takuya , Yagi Yoshiki , Kishimoto Masafumi , Shintani Hiroshi , Hayashi Yasuyuki , Sogabe Taku , Morooka Takaya , Sakamoto Haruko , Suzuki Keitaro , Nakamura Fumiko , Nishioka Norihiro , Matsuyama Tasuku , Sado Junya , Matsui Satoshi , Shimazu Takeshi , Koike Kaoru , Kawamura Takashi , Kitamura Tetsuhisa , Iwami Taku 
Published: May 2020

Abstract
Background
We aimed to identify the association of pH value in blood gas assessment with neurological outcome among out-of-hospital cardiac arrest (OHCA) patients treated by extracorporeal cardiopulmonary resuscitation (ECPR).

Methods
We retrospectively analyzed the database of a multicenter prospective observational study on OHCA patients in Osaka prefecture, Japan (CRITICAL study), from July 1, 2012 to December 31, 2016. We included adult OHCA patients treated by ECPR. Patients with OHCA from external causes such as trauma were excluded. We conducted logistic regression analysis to identify the odds ratio (OR) and 95% confidence interval (CI) of the pH value for 1 month favorable neurological outcome adjusted for potential confounders including sex, age, witnessed by bystander, CPR by bystander, pre-hospital initial cardiac rhythm, and cardiac rhythm on hospital arrival.

Results
Among the 9822 patients in the database, 260 patients were finally included in the analysis. The three groups were Tertile 1: pH ≥ 7.030, Tertile 2: pH 6.875–7.029, and Tertile 3: pH < 6.875. The adjusted OR of Tertiles 2 and 3 compared with Tertile 1 for 1 month favorable neurological outcome were 0.26 (95% CI 0.10–0.63) and 0.24 (95% CI 0.09–0.61), respectively.

Conclusions
This multi-institutional observational study showed that low pH value (< 7.03) before the implementation of ECPR was associated with 1 month unfavorable neurological outcome among OHCA patients treated with ECPR. It may be helpful to consider the candidate for ECPR.
Gamma detector dead time correction using Lambert W function
Authors: Heemskerk Jan W. T. , Defrise Michel 
Published: May 2020

AbstractBackgroundFor therapeutic applications of several isotopes (e.g.,131I,153Sm,177Lu) in nuclear medicine, the high activities typically applied require accurate dead time correction in early time point imaging. We present a novel, straightforward dead time correction method using the LambertWfunction, which is in principle exact for the paralyzable detector model with a single parameter τ (i.e., dead time).ResultsAs a proof of concept, the method is validated with a simple model: a commonly used isotope,99mTc, with a single photopeak. We measured count rates of a gamma camera both intrinsically and extrinsically (i.e., with collimators) with point sources in air and in a scatter phantom (extrinsic only). τ was estimated for both open window (τOW) and a99mTc photopeak window (τTc), using a “graphical” method for fitting the count rate of decaying sources. These values for τ were subsequently used for dead time correction.τ varied significantly between the different geometries for both energy windows, but τOWwas more reproducible than τTc, particularly for the scatter phantom measurements.τOWmeasured from the phantom measurements was approximately 30% lower than τOWfrom the intrinsic measurement but corresponded within 15% with the extrinsic point source measurements. Accordingly, using the intrinsic τOWled to an overcorrection of 8% at high count rates; τOWfrom the extrinsic point source measurements corrected the phantom measurement to within 2%.However, significant differences were observed between τTcvalues. All measured τTcvalues underestimated dead time losses in a second independent phantom measurement, with even τTcfrom the first phantom measurement underestimating activity with 5–10% at the highest count rates. Based on measurements of the effect of energy window settings and geometry, we tentatively attribute the added dead time losses to pulse pile-up.ConclusionsAnalytic dead time correction based on the LambertWfunction is accurate for the range in which gamma detectors behave as paralyzable systems. However, further investigation indicated measured τ values to be variable with geometry as well as window fraction. We propose that dead time correction should be based on the open window value, τOW, corrected for window fraction.
Laparoscopic and endoscopic cooperative surgery for gastric cancer mimicking a submucosal tumor
Authors: Ozawa Hiroki , Kawakubo Hirofumi , Matsuda Satoru , Mayanagi Shuhei , Irino Tomoyuki , Fukuda Kazumasa , Nakamura Rieko , Wada Norihito , Kitagawa Yuko 
Published: May 2020

Abstract

Background
Gastric cancer that mimics a submucosal tumor (SMT) is infrequently encountered in routine clinical settings, and histopathological analysis is often negative for malignant cells. In such cases, excisional biopsy of the entire tumor may be necessary to make a definitive pathological diagnosis, and laparoscopic and endoscopic cooperative surgery (LECS) is a viable method of excisional biopsy.

Case presentation
An 80-year-old male patient diagnosed with stomach wall irregularities at routine medical check-up was referred to our facility, and consequent endoscopic examination detected a 20-mm protruded lesion in the greater curvature at the middle third of the stomach. Endoscopic ultrasound (EUS) showed a thick, low echoic lesion with an irregular margin in the second layer of the gastric wall. Further, a nodular part of the lesion had infiltrated into the submucosa, with an appearance similar to that of linitis plastica of the stomach. The lesion was highly suspected to be a gastric carcinoma with submucosal invasion. However, mucosal-incision-assisted biopsy revealed no malignant cells. Computed tomography (CT) identified no metastatic lymph nodes. Therefore, an excisional biopsy using LECS was performed, and to avoid peritoneal dissemination, we used a modified version, namely, combination of laparoscopic and endoscopic approaches to neoplasia with non-exposure technique (CLEAN-NET). The procedure ended without any complications, and post-operative course was uneventful. As histopathology returned a diagnosis of adenocarcinoma pT4a, we performed radical gastrectomy and D2 lymphadenectomy. Post-operative course was unremarkable and the patient underwent follow-up examinations without adjuvant chemotherapy because of old age.

Conclusions
Local resection using LECS for gastric tumors with a high suspicion of malignancy is useful and feasible. LECS could be used in similar cases.
Integrating automatic question generation with computerised adaptive test
Authors: Susanti Yuni , Tokunaga Takenobu , Nishikawa Hitoshi 
Published: May 2020

AbstractThe present study focuses on the integration of an automatic question generation (AQG) system and a computerised adaptive test (CAT). We conducted two experiments. In the first experiment, we administered sets of questions to English learners to gather their responses. We further used their responses in the second experiment, which is a simulation-based experiment of the AQG and CAT integration. We proposed a method to integrate them with a predetermined item difficulty that enables to integrate AQG and CAT without administering the items in a pretesting. The result showed that all CAT simulations performed better than the baseline, a linear test, in estimating the test taker’s true proficiency.
Establishment of an extensive breeding population of a marine pulmonate snail far poleward of its previously documented range
Authors: Fork Susanne , Pernet Bruno , Wasson Kerstin 
Published: May 2020

AbstractThe California salt marsh snailMelampus olivaceus, a species from southern California, has established a thriving population in the Elkhorn Slough estuary (Monterey Bay), hundreds of kilometers north of where it is long-established and regularly found. Since the late 1800s this high littoral zone salt marsh snail has occasionally been reported in central California, but only as isolated individuals or shells. We first observed a few individuals ofM. olivaceusin Elkhorn Slough in 2013, and the species is now abundant and reproducing throughout the estuary inSalicornia pacifica(pickleweed) marshes. We also document for the first time that this species has a planktonic, feeding larva. Salt marsh snails may have been transported northward as planktonic larvae during periodic El Niño warm water events. Such transport has been reported for other species, but what makes this case unusual is the persistence, reproduction and abundance of the snail following transport.
Simultaneous estimation for Dicyclomine HCl and Simethicone in bulk and oral liquid drop formulation: an RP-HPLC method development and validation
Authors: Pandey Pradeep Kumar , Patel Mukesh , Manigauha Ashish , Wadhwa Pankaj , Sahu Sanjeev Kumar 
Published: May 2020

AbstractBackgroundA new, validated, and selective RP-HPLC technique was established for quantitative estimation of Dicyclomine HCl and Simethicone in the pharmaceutical oral liquid drop formulations. The chromatographic conditions were adjusted using Luna C18 (25 cm × 0.46 cm, 5 μm) column, water-methanol mixture (80:20, v/v) as solvent system and pH adjustment up to 7.4 using orthophosphoric acid. The absorbance was observed at 289 nm λmax injecting 20 μl sample solution and maintain the ambient temperature (30 °C) with 1 ml per min flow rate.ResultsThe retention times of Dicyclomine HCl and Simethicone were determined 2.962 and 4.091 min, respectively. The developed analytical technique was validated for accuracy, precision, linearity, specificity, sensitivity, ruggedness, and robustness as per ICH guidelines. The LOD was found to be 0.72 and 0.57 μg per ml for Dicyclomine HCl and Simethicone, respectively. But, LOQ was 2.19 and 1.73 μg per ml, respectively.ConclusionsThis validated method showed good precision (RSD% < 1) with acceptable linearity (≥ 0.999). Intra- and inter-day relative RSD of retention times and AUC (area under curve) were found to be less than 2.0%. The method was also significantly useful as economic technique for simultaneous quantitative determination of Dicyclomine HCl and Simethicone in the bulk and pharmaceutical formulation.
Seasonality in schizophrenia—An analysis of a nationwide registry with 110,735 hospital admissions
Authors: Hinterbuchinger B. , König D. , Gmeiner A. , Listabarth S. , Fellinger M. , Thenius C. , Baumgartner J. S. , Vyssoki S. , Waldhoer T. , Vyssoki B. , Pruckner N. 
Published: May 2020

Abstract

Background.
Seasonal patterns in hospitalizations have been observed in various psychiatric disorders, however, it is unclear whether they also exist in schizophrenia. Previous studies found mixed results and those reporting the presence of seasonality differ regarding the characteristics of these patterns. Further, they are inconclusive whether sex is an influencing factor. The aim of this study was therefore to examine if seasonal patterns in hospitalizations can be found in schizophrenia, with special regard to a possible influence of sex, by using a large national dataset.

Methods.
Data on all hospital admissions within Austria due to schizophrenia (F20.0–F20.6) for the time period of 2003–2016 were included. Age standardized monthly variation of hospitalization for women and men was analyzed and the level of significance adjusted for multiple testing.

Results.
The database comprised of 110,735 admissions (59.6% men). Significant seasonal variations were found in the total sample with hospitalization peaks in January and June and a trough in December (p < 0.0001). No significant difference in these patterns was found between women and men with schizophrenia (p < 0.0001).

Conclusion.
Our study shows that schizophrenia-related hospitalizations follow a seasonal pattern in both men and women. The distribution of peaks might be influenced by photoperiod changes which trigger worsening of symptoms and lead to exacerbations in schizophrenia. Further research is necessary to identify underlying factors influencing seasonal patterns and to assess whether a subgroup of patients with schizophrenia is especially vulnerable to the impact of seasonal variations.
Non-caveolar caveolins – duties outside the caves
Authors: Pol Albert , Morales-Paytuví Frederic , Bosch Marta , Parton Robert G. 
Published: May 2020

ABSTRACT
Caveolae are invaginations of the plasma membrane that are remarkably abundant in adipocytes, endothelial cells and muscle. Caveolae provide cells with resources for mechanoprotection, can undergo fission from the plasma membrane and can regulate a variety of signaling pathways. Caveolins are fundamental components of caveolae, but many cells, such as hepatocytes and many neurons, express caveolins without forming distinguishable caveolae. Thus, the function of caveolins goes beyond their roles as caveolar components. The membrane-organizing and -sculpting capacities of caveolins, in combination with their complex intracellular trafficking, might contribute to these additional roles. Furthermore, non-caveolar caveolins can potentially interact with proteins normally excluded from caveolae. Here, we revisit the non-canonical roles of caveolins in a variety of cellular contexts including liver, brain, lymphocytes, cilia and cancer cells, as well as consider insights from invertebrate systems. Non-caveolar caveolins can determine the intracellular fluxes of active lipids, including cholesterol and sphingolipids. Accordingly, caveolins directly or remotely control a plethora of lipid-dependent processes such as the endocytosis of specific cargoes, sorting and transport in endocytic compartments, or different signaling pathways. Indeed, loss-of-function of non-caveolar caveolins might contribute to the common phenotypes and pathologies of caveolin-deficient cells and animals.
FAZ27 cooperates with FLAM3 and ClpGM6 to maintain cell morphology in Trypanosoma brucei
Authors: An Tai , Zhou Qing , Hu Huiqing , Cormaty Harshini , Li Ziyin 
Published: May 2020

ABSTRACT
The human parasite Trypanosoma brucei transitions from the trypomastigote form to the epimastigote form in the insect vector by repositioning its mitochondrial genome and flagellum-associated cytoskeleton. The molecular mechanisms underlying such changes in cell morphology remain elusive, but recent works demonstrated the involvement of three flagellar proteins, FLAM3, ClpGM6 and KIN-E, in this process by controlling the elongation of the flagellum attachment zone (FAZ). In this report, we identified a FAZ flagellum domain-localizing protein named FAZ27 and characterized its role in cell morphogenesis. Depletion of FAZ27 in the trypomastigote form caused major morphological changes and repositioning of the mitochondrial genome and flagellum-associated cytoskeleton, generating epimastigote-like cells. Furthermore, proximity biotinylation and co-immunoprecipitation identified FLAM3 and ClpGM6 as FAZ27-interacting proteins, and analyses of their functional interplay revealed an interdependency for assembly into the FAZ flagellum domain. Finally, we showed that assembly of FAZ27 occurred proximally, identical to the assembly pattern of other FAZ sub-domain proteins. Taken together, these results demonstrate a crucial role for the FAZ flagellum domain in controlling cell morphogenesis and suggest a coordinated assembly of all the FAZ sub-domains at the proximal end of the FAZ.
Observation of Translational Diffusion in a Planer Supported Lipid Bilayer Membrane by Total Internal Reflection-Transient Grating Method
Authors: Kondoh Masato , Moritani Hidekazu , Ishibashi Taka-aki 
Published: May 2020

Abstract
Total internal reflection-transient grating (TIR-TG) method has been applied to diffusion coefficient measurements of trans-stilbene in a dimyristoylphosphatidylcholine (DMPC) lipid bilayer membrane at a silica/water interface. The observed TIR-TG signal consisted of four decay components. The rate constants of the third and fourth components quadratically depended on grating wavenumbers, which indicates that these components represent diffusion processes of trans-stilbene (diffusion signals). From the decay rate constants of the diffusion signals, two different diffusion coefficients, (5.7 ± 1.0) × 10−10 and (2.8 ± 0.5) × 10−12 m2s−1 were obtained for trans-stilbene in the DMPC membrane. This result indicates that the DMPC membrane is an inhomogeneous environment that can be described with two different effective viscosities.