bluuebunny/crossref_metadata_2025_split huggingface dataset:
FABP4 promotes invasion and metastasis of colon cancer by regulating fatty acid transport
Authors: Tian Wenying , Zhang Wenjia , Zhang Yan , Zhu Tianyue , Hua Yuting , Li Hui , Zhang Qinglin , Xia Min 
Published: October 2020

Abstract
Background
The prognosis of colon cancer is poor for metastasis, while the mechanism, especially adipocytes related, is not yet clear. The purpose of this study is to determine the effects of fatty acid binding protein 4 (FABP4), a transporter for lipids, on colon cancer progression.

Methods
The distribution of lipids and FABP4 was tested in the colon cancer tissues and adjacent normal tissues, and their relationship was also verified in vitro. Experiments about cellular invasion, migration and proliferation were performed to detect the impacts of FABP4 on the biological behaviors of colon cancer, and the positive results were checked in vivo. Meanwhile, the regulatory role of FABP4 in the energy and lipid metabolism was evaluated by the levels of triglyceride, ATP, LDH, glycerol and NEFA. At last, GO and KEGG analysis based on FABP4 overexpressed cells was performed, and the AKT pathway and epithelial–mesenchymal transition (EMT)-related proteins were determined by Western blot.

Results
Higher accumulation of lipids and stronger FABP4 transcription were observed in colon cancer tissues. Having been incubated with adipose tissue extract and overexpressed FABP4, colon cancer cells demonstrated enhanced lipid accumulation. In functional experiments, co-culture with adipose tissue extract significantly enhanced the invasion and migration of colon cancer cells, as well as the energy and lipid metabolism, and all these processes were reversed by FABP4 inhibitor. In addition, the metastasis of FABP4-overexpressed colon cancer cells was also significantly enhanced in vitro and in vivo. In terms of mechanism, the bioinformatics analysis showed that FABP4 was enriched in 11 pathways related to metabolic processes in FABP4 overexpressed cells. Finally, FABP4 overexpression improved EMT progression of colon cancer, as evidenced by the upregulation of Snail, MMP-2 and MMP-9, the downregulation of E-cadherin. The expression of p-Akt was also elevated.

Conclusion
FABP4 overexpression could increase FAs transport to enhance energy and lipid metabolism, and activate AKT pathway and EMT to promote the migration and invasion of colon cancer cells.
Addressing the caste system in U.S. healthcare in the era of COVID-19
Authors: Sivashanker Karthik , Couillard Cheri , Goldsmith Jennifer , Walker Normella , Eappen Sunil 
Published: October 2020

AbstractIn healthcare, we find an industry that typifies the unique blend of racism, classism, and other forms of structural discrimination that comprise the U.S. caste system—the artificially-constructed and legally-reinforced social hierarchy for assigning worth and determining opportunity for individuals based on race, class, and other factors. Despite myths of meritocracy, healthcare is actually a casteocracy; and conversations about racism in healthcare largely occupy an echo chamber among the privileged upper caste of hospital professionals. To address racism in healthcare, we must consider the history that brought us here and understand how we effectively perpetuate an employee caste system within our own walls.
Sensory neurons from dorsal root ganglia regulate endothelial cell function in extracellular matrix remodelling
Authors: Leroux Alice , Paiva dos Santos Bruno , Leng Jacques , Oliveira Hugo , Amédée Joëlle 
Published: October 2020

AbstractBackgroundRecent physiological and experimental data highlight the role of the sensory nervous system in bone repair, but its precise role on angiogenesis in a bone regeneration context is still unknown. Our previous work demonstrated that sensory neurons (SNs) induce the osteoblastic differentiation of mesenchymal stem cells, but the influence of SNs on endothelial cells (ECs) was not studied.MethodsHere, in order to study in vitro the interplay between SNs and ECs, we used microfluidic devices as an indirect co-culture model. Gene expression analysis of angiogenic markers, as well as measurements of metalloproteinases protein levels and enzymatic activity, were performed.ResultsWe were able to demonstrate that two sensory neuropeptides, calcitonin gene-related peptide (CGRP) and substance P (SP), were involved in the transcriptional upregulation of angiogenic markers (vascular endothelial growth factor, angiopoietin 1, type 4 collagen, matrix metalloproteinase 2) in ECs. Co-cultures of ECs with SNs also increased the protein level and enzymatic activity of matrix metalloproteinases 2 and 9 (MMP2/MMP9) in ECs.ConclusionsOur results suggest a role of sensory neurons, and more specifically of CGRP and SP, in the remodelling of endothelial cells extracellular matrix, thus supporting and enhancing the angiogenesis process.
Increased percentage of PD-L1+ natural killer cells predicts poor prognosis in sepsis patients: a prospective observational cohort study
Authors: Jiang Wenqiang , Li Xusheng , Wen Miaoyun , Liu Xiaoyu , Wang Kangrong , Wang Qiaosheng , Li Ya , Zhou Maohua , Liu Mengting , Hu Bei , Zeng Hongke 
Published: October 2020

Abstract
Background
Natural killer (NK) cells play a major role in immune tolerance after sepsis, and the programmed cell death 1 (PD-1) and programmed cell death ligand 1 (PD-L1) system mediates evasion of host immunity. The correlation between PD-L1 levels in NK cells and the prognosis of patients with sepsis, however, has not been elucidated. Thus, it was hypothesized that PD-L1 in NK cells could be a novel biomarker of the mortality for sepsis patients.

Methods
A prospective, observational, cohort study in a general intensive care unit had earlier enrolled patients according to the sepsis-3 criteria, and peripheral blood samples were collected within 24 h post-recruitment. The expression of four co-signaling molecules (PD-1, CD28, PD-L1, and CD86) in NK cells was assayed, and the sequential organ failure assessment (SOFA) scores were recorded on day 1. Patients were followed up until 28 days. Multivariate regression analysis assessed the independent risk factors for 28-day mortality. The association between biomarkers and 28-day mortality was assessed by Cox regression survival analysis. The accuracy of biomarkers for mortality was determined by the area under the receiver operating characteristic (ROC) curve (AUC) analysis.

Results
A total of 269 patients were recruited, and 114 patients were finally included for final analysis. Of these, 30 (26.3%) patients died during 28 days. The percentage of PD-L1+ NK cells (OR 1.022; 95% CI 1.002–1.043) and SOFA scores (OR 1.247; 95% CI 1.092–1.424) were independent risk factors for 28-day mortality. The AUC of the percentage of PD-L1+ NK cells, SOFA scores, and their combination model were 0.655 (0.559–0.742), 0.727 (0.635–0.807) and 0.808 (0.723–0.876), respectively. The combination model was the indicator with the best AUC to predict mortality in 28 days (all p < 0.05). Patients with the percentage of PD-L1+ NK cells above the cutoff point 5.58% (hazard ratio (HR) 10.128 (1.372–74.772), p = 0.001), and the combination model prediction possibility above 0.1241 (HR 13.730 (3.241–58.158), p < 0.001) were the indexes that had greater discriminative capacity to predict 28 days mortality.

Conclusions
The percentage of PD-L1+ NK cells at admission serves as a novel prognostic biomarker for predicting mortality and contributes to improve the predictive capacity of SOFA score in patients with sepsis.
The use of venous Doppler to predict adverse kidney events in a general ICU cohort
Authors: Spiegel Rory , Teeter William , Sullivan Scott , Tupchong Keegan , Mohammed Nabeel , Sutherland Mark , Leibner Evan , Rola Philippe , Galvagno Samuel M. , Murthi Sarah B. 
Published: October 2020

Abstract
Background
Changes in Doppler flow patterns of hepatic veins (HV), portal vein (PV) and intra-renal veins (RV) reflect right atrial pressure and venous congestion; the feasibility of obtaining these assessments and the clinical relevance of the findings is unknown in a general ICU population. This study compares the morphology of HV, PV and RV waveform abnormalities in prediction of major adverse kidney events at 30 days (MAKE30) in critically ill patients.

Study design and methods
We conducted a prospective observational study enrolling adult patients within 24 h of admission to the ICU. Patients underwent an ultrasound evaluation of the HV, PV and RV. We compared the rate of MAKE-30 events in patients with and without venous flow abnormalities in the hepatic, portal and intra-renal veins. The HV was considered abnormal if S to D wave reversal was present. The PV was considered abnormal if the portal pulsatility index (PPI) was greater than 30%. We also examined PPI as a continuous variable to assess whether small changes in portal vein flow was a clinically important marker of venous congestion.

Results
From January 2019 to June 2019, we enrolled 114 patients. HV abnormalities demonstrate an odds ratio of 4.0 (95% CI 1.4–11.2). PV as a dichotomous outcome is associated with an increased odds ratio of MAKE-30 but fails to reach statistical significance (OR 2.3 95% CI 0.87–5.96), but when examined as a continuous variable it demonstrates an odds ratio of 1.03 (95% CI 1.00–1.06). RV Doppler flow abnormalities are not associated with an increase in the rate of MAKE-30

Interpretation
Obtaining hepatic, portal and renal venous Doppler assessments in critically ill ICU patients are feasible. Abnormalities in hepatic and portal venous Doppler are associated with an increase in MAKE-30. Further research is needed to determine if venous Doppler assessments can be useful measures in assessing right-sided venous congestion in critically ill patients.
An optimized secretory expression system and immunogenicity evaluation for glycosylated gp90 of avian reticuloendotheliosis virus
Authors: Pan Qing , Wang Jing , Hussain Altaf , Gao Yulong , Cui Hongyu , Li Kai , Qi Xiaole , Liu Changjun , Zhang Yanping , Zhang Shide , Wang Xiaomei 
Published: October 2020

AbstractReticuloendotheliosis is an important immunosuppressive disease, associated with avian reticuloendotheliosis virus (REV) infection, and causes notable economic losses worldwide. Glycoprotein gp90 is an important structural protein of REV, and considered to be the most important immunogenic antigen, which can induce neutralizing antibodies against REV. In this study, an optimized suspension culture system was developed and applied to secretory express the immunogenic surface antigen gp90. To achieve an optimal glycosylation, the gp90 was designed to secretory expressed into the supernatant of the cell culture, which also occurs in the natural protein maturation procedure of REV. Serum-free culture medium was introduced to simplify the purification process and reduce the production costs. Based on the purified glycosylated gp90, an oil-emulsion subunit REV vaccine candidate was developed and evaluated in chickens. The subunit gp90-based vaccine induced fast immune responses, high levels of antibodies (REV-specific antibody, gp90-specific antibody, and neutralizing antibody against REV), and preferential T helper 2 (Th2) (interleukin-4 secretion) not Th1 (interferon-γ secretion) response. Furthermore, the viremia induced by REV infection was significantly reduced in chickens immunized with the glycosylated gp90. Overall, an optimized secretory expression system for glycosylated gp90 was developed, and the glycosylated gp90 obtained in this study retained good immunogenicity and could be an attractive vaccine candidate to protect chickens against REV horizonal infection.
Pretreatment Primary Tumor Stage is a Risk Factor for Recurrence in Patients with Esophageal Squamous Cell Carcinoma Who Achieve Pathological Complete Response After Neoadjuvant Chemoradiotherapy
Authors: La Mendola Roberta , Bencivenga Maria , Torroni Lorena , Alberti Luca , Sacco Michele , Casella Francesco , Ridolfi Cecilia , Simoni Nicola , Micera Renato , Pavarana Michele , Verlato Giuseppe , Giacopuzzi Simone 
Published: October 2020

Abstract
Background
Although pathological complete response (pCR) after multimodal treatment for esophageal cancer is associated to the best prognosis, recurrence may occur in 20–40% of cases. The present study investigated the recurrence pattern and predictive factors of recurrence after pCR in patients with esophageal cancer.

Methods
In this study, 427 patients received preoperative treatment for either esophageal squamous cell carcinoma (SCC) or adenocarcinoma at Verona University Hospital between 2000 and 2018. Of these, 145 patients (34%) achieved a pCR. Long-term prognosis, recurrence pattern, and risk factors for relapse in pCR patients were analysed.

Results
During a median follow-up of 52 months, 37 relapses (25.5%) occurred, mostly at distant level (n = 28). Nearly all locoregional relapses (8/9) were detected in SCC cases. The 5-year overall survival and cancer-related survival were 71.7% (95% confidence interval [CI] 62.6–78.9%) and 77.5% (95% CI 68.5–84.2%) respectively. Male sex, higher body mass index, and cT4 were significant risk factors for recurrence at univariate analysis. The multivariate analysis confirmed the role of cT4 as predictor of recurrence only in SCCs.

Conclusions
Esophageal cancer recurs in about one-fourth of pCR cases. A fair number of local recurrences occurs in SCCs, but the main problem is the systemic disease control. According to our analysis, SCCs patients with cT4 stage have an increased risk to recur, so they should be managed differently by a personalized approach in terms of adjuvant treatment and follow-up.
Interactions of Ruthenium(III) Complexes Bearing Bis(2-pyridylcarbonyl)aminate with Cations at the Carbonyl Group
Authors: Misawa-Suzuki Tomoyo , Etoh Mami , Nagao Hirotaka 
Published: October 2020

Abstract
Bis(2-pyridylcarbonyl)aminate (bpca) coordinates to a ruthenium center with three nitrogen atoms of two pyridyl and one aminate group and the biscarbonylaminate moiety ({O-C-N-C-O}−) functions as an alternative coordination site. Interactions of the carbonyl groups of bis(2-pyridylcarbonyl)aminato with cations (Mz+; Li+, Na+, K+, and Mg2+) were investigated using chloridoacetonitrileruthenium(III) complexes in three different charges, [RuIIICl3−n(NCCH3)n(bpca)]m (n = 0, m = −1; [1]−, n = 1, m = 0; [2], n = 2, m = +1; [3]+). While the interactions of the Ru(III)-bpca moiety with divalent cation, Mg2+, were observed, monovalent cations, Li+ and Na+, interacted with the Ru-bpca moiety in the Ru(III) state or accompanied by one-electron reduction via a metal ion-coupled electron transfer (MCET). The interactions with cations were related to the electronic structure of the Ru(III)-bpca moiety which was influenced by the electrostatic nature of three co-existing ligands, the charge of the complex and the charge density of Mz+.
Langmuir-Blodgett Films of Chiral Perfluorinated Gelators: Effects of Chirality and Chain Length on Two-Dimensional Behavior
Authors: Yamagishi Akihiko , Umemura Yasushi , Tamura Kenji , Yajima Tomoko , Sato Hisako 
Published: October 2020

Abstract
Surface pressure versus molecular area (π-A) curves were measured on pure water as a subphase for a series of N,N′-diperfluoroalkanoyl-1,2(R,R)-diaminocyclohexanes. A molecule is denoted as RR-CFn, where n is the number of carbon atoms in a perfluoroalkanoyl chain (or n − 2 = the number of difluoromethylene units). The chain length was varied for n = 4, 5, 6, 8, 9, and 10. The results for n = 7 were reported previously. The effects of chain length and optical purity on film formation were investigated. The surface morphology of a film deposited onto a hydrophilic glass plate was observed using an atomic force microscope (AFM). For n = 4, 5, and 6, the floating films were already multilayered before compression and the deposited films were composed of rectangular or rod-like aggregates. For n = 7, 8, 9, and 10, monolayered films were formed and underwent structural transformation upon compression. From the AFM images, the films deposited after the transformation were composed of fiber-like aggregates. For a racemic mixture, no monolayer film was formed, and the film transfer was impossible irrespective of the chain length. p-Polarized infrared multiple angle incidence resolution spectrometry (pMAIRS) measurements were carried out on a film of RR-CF8 deposited onto a silicon wafer to determine the orientation of the composite molecules. The results were compared with the monolayer behavior reported for a compound having a single perfluoroalkyl chain. The relation to their gelation behavior is also discussed.
Detection of Spermine Using Cucurbit[7]uril-phenazopyridine Host-Guest Inclusion Complex as a Platform
Authors: Zhang Huaqing , Liu Mei , Zhu Xiaofei , Li Hui 
Published: October 2020

Abstract
We proposed a competitive method for rapid and sensitive detection of spermine using CB[7]-PHE (CB[7] = cucurbit[7]uril, PHE = phenazopyridine) as a platform. The LOD was determined to be 21 nM and showed good calibration in the concentration range of 0.071–14 µM, which is sufficiently below the critical concentration for cancer diagnosis. More interestingly, the addition of spermine to CB[7]-PHE led to color change from light brown to yellow, which indicating potential applications for colorimetric analysis of spermine.
Tres casos de periodismo narrativo en México impulsados por la crisis de las instituciones gubernamentales
Authors: Vargas Pasaye Rafael Gerardo 
Published: October 2020

La sociedad en México ha perdido parte de la credibilidad y la confianza de algunas instituciones gubernamentales en buena medida porque estas no dan respuesta a sus necesidades, y se ven rebasadas en ámbitos relacionados con corrupción, procuración de justicia y seguridad. En esos espacios, el periodismo narrativo actual ha descrito las injusticias y consecuencias de la crisis que representa dicho debilitamiento. Este texto analiza tres piezas que, por su oportunidad, relevancia y reconocimiento público, lo ejemplifican: El cártel de Sinaloa de Diego Enrique Osorno (2009), La fosa de agua de Lydiette Carrión (2017) y La estafa maestra de Nayeli Roldán, Miriam Castillo y Manuel Ureste (2018). Selección que responde a la relevancia y el reconocimiento público, ya que, además de oportunos, llegaron a convertirse incluso en un parámetro comparativo que forma parte del lenguaje cotidiano. Se recurre al método hermenéutico bajo la lógica de su forma de trabajo: interpretación del texto, consideración del contexto y conclusión, para lo cual se concluye con una generación de categorías para los tres casos: elementos de denuncia, pruebas, testimonios, seguimiento y metáforas, por lo que se pueden analizar como un conjunto de piezas perteneciente a un contexto mexicano. Se concluye que el debilitamiento de las instituciones en México con índices de calificación más bajos por abuso de autoridad, ausencia de resultados y exceso de corrupción, por ejemplo, incrementaron un vacío de poder que detonó parte del periodismo narrativo que ahora se observa, y que también refleja las consecuencias de esa crisis.
La canción en el rock argentino, Almendra, un comienzo
Authors: Goldsack Elina 
Published: October 2020

El presente trabajo se ocupa de las canciones de un disco fundacional en la historia del rock en la argentina: Almendra. Grabado entre abril y septiembre de 1969 en los estudios T.N.T. y editado por RCA, Sony Music, el disco contiene nueve canciones caracterizadas por el eclecticismo poético y musical. Considerando este disco como una bisagra de la canción popular argentina, nos proponemos indagar fundamentalmente en la relación texto-música. Poniendo el foco en ese vínculo complejo donde se potencian el decir de la palabra y el decir de los sonidos.
¿Dónde radica la innovación en relación a este vínculo? ¿En particularidades métricas? ¿En formas que no responden al esquema estrofa-estribillo? ¿En armonías o melodías que enfaticen estas particularidades? A partir del análisis musical focalizado en estos aspectos pretendemos encontrar algunas respuestas.
Esta investigación se encuentra enmarcada en el CAI+D 2016 «Cambios en innovaciones en la canción popular argentina a partir de los ‘60. Desafíos a las categorías vigentes» que se desarrolla en el Instituto Superior de Música de la Universidad Nacional del Litoral.
La experiencia narrada como vehículo de construcción colectiva en la música de Luc Ferrari
Authors: Reyna Alejandro 
Published: October 2020

El artículo propone interpretar la experiencia narrada que aparece en la música de Ferrari como una búsqueda por articular un sentido colectivo. En otras palabras, toda la exposición de la vida personal del compositor que aparece en las obras posee un sentido que excede la simple anécdota personal. Luego de plantear una definición de experiencia considerada pertinente en relación a nuestros objetivos, en un primer momento se habla de la obra Hétérozygote y del field recording como «gatillo» de experiencias. A continuación, nos abocamos a reflexionar sobre las historias compartidas por Ferrari. Finalmente, en el apartado «Lo colectivo en el gesto de contarse», se analiza en términos colectivos la exposición personal que propone Ferrari, tomando como ejemplo la obra Chantal, ou le portrait d’une villageoise.
Músicas populares en debates académicos, o ¿cada carancho a su rancho?
Authors: Prendes Marcia Natividad 
Published: October 2020

En el presente trabajo se presentan algunas reflexiones relacionadas a la investigación que la autora se encuentra desarrollando en el marco de sus estudios de posgrado. Se propone analizar ideas, conceptos y perspectivas metodológicas y críticas contenidas en algunos textos y discusiones sobre músicas populares argentinas - latinoamericanas. Dichos estudios sostienen que resulta difícil insertar y sostener la música y la musicología popular en los ámbitos académicos, así como producir sistematizaciones válidas para su enseñanza. Insisten en que si no se considera su especificidad, o no se abordan bajo sus propios cánones y procesos de producción característicos, se siguen reproduciendo modelos pedagógicos pensados para las músicas dominantes, bajo un planteo de supuesta “universalidad”, donde todo se vuelve analizable desde la perspectiva de la música académica. Aquellas músicas que por omisión son silenciadas, negadas, no reconocidas, prohibidas o censuradas, quedan excluidas del sistema y los ámbitos académicos, subestimando (en su reducción y simplificación) al objeto de estudio y su propia especificidad.