bluuebunny/crossref_metadata_2025_split huggingface dataset:
Essential oil from <i>Chenopodium ambrosioides L.</i> induces mitochondrial-mediated pathway and endoplasmic reticulum stress-related apoptosis in human liver cancer SMMC-7721 cells
Authors: Rui-Hua Qian , Xiao-Huan Zhu , Jun-Mei Hao , Jun-Mei Hao , Hai-Ying Li , Bo-Han Song , Qiu-Rong Ren , Zhao-Bin Chen , Zhao-Bin Chen , Dan-Wei Ma , Ya-Nan Wang 
Published: June 2020

Purpose: To evaluate the cytotoxic effect of essential oil derived from Chenopodium ambrosioides L. in Sichuan Province on human liver cancer SMMC-7721 cells, as well as its possible molecular mechanisms.Methods: Cytotoxicity was characterized by MTT assay and transmission electron microscopy (TEM) of SMMC-7721 cells ultrastructure. The apoptotic effect of the essential oil was evaluated by changes in mitochondrial membrane potential and Western blot assay.Results: MTT assay data indicate that the essential oil was cytotoxic to SMMC-7721 cells, while TEN revealed that there were vacuoles and nucleus fragmentation in the SMMC-7721 cell cytosol, cell swelling, and a large amount of leakage. Mitochondrial membrane potential assay and Western Blot data indicate that the essential oil induced cell apoptosis.Conclusion: The essential oil of Chenopodium ambrosioides L. in Sichuan Province seems to induce apoptosis of human liver cancer SMMC-7721 cells via the mitochondrial-mediated pathway and endoplasmic reticulum stress. Thus, this plant requires further investigation as a potential source of ananti-liver cancer drug.
Keywords: Chenopodium ambrosioides Essential oil; Anti-tumor activity, Liver cancer Apoptosis, SMMC-7721 cells
Engelharquinone suppresses lipopolysaccharide-induced inflammation and proliferation of human liver cancer SMCC7721 cells via inhibition of NF-κB and MAPK signaling pathway
Authors: Li Shan , Zhang Yan , Gao Aishe , Zhang Yue , Zhang Jiong 
Published: June 2020

Purpose: To investigate the anti-tumor effect of engelharquinone (Eng) on human liver cancer SMCC7721 cells.Methods: GFP-labeled SMCC7721 cells were used to establish a tumor-bearing mice model used for determination of the effect of different concentrations of Eng on tumor growth. The effect of Eng on SMCC7721 cell viability was determined with MTT assay and cell cycle analysis. The anti-inflammatory effect of Eng on lipopolysaccharide (LPS)-treated SMCC7721 cells were determined through assay of pro-inflammatory cytokines. Besides, the effect of Eng on the expressions of mitogen-activated protein kinase (MAPK), toll-like receptor 4 (TLR4), and nuclear factor kappa B (NF-κB) was determined.Results: Cell proliferation was suppressed by different concentrations of Eng in LPS-treated SMCC7721 cells. Treatment of nude mice with Eng at high and low doses resulted in significant suppression of tumor growth and marked increases in percentage survival. Treatment of SMCC7721 cells with LPS upregulated the expressions of NF-κB, p65 and MAPK. However, pre-treatment of the cells with Eng suppressed the LPS-induced upregulation of the NF-κB, p65 and MAPK signaling pathways, and further downregulated the production of inflammatory cytokines in SMCC7721 cells.Conclusion: These results indicate that Eng inhibits LPS-induced inflammation and proliferation of human liver cancer SMCC7721 cells via a mechanism involving regulation of NF-κB and MAPK signaling pathways. Thus, Eng has potentials for the clinical management of inflammatory diseases and liver cancer.
Keywords: Inflammation, Engelharquinone, Lipopolysaccharide, SMCC7721 cells, Toll-like receptor 4
Contribution of Gender on Learning Readiness among School Students of Nepal
Authors: Dangol Rita , Shrestha Milan 
Published: June 2020

Readiness is the prerequisite for the effective learning process among school students. Its absence can hinder the learning of the students and schooling as a whole. The purpose of this study is to examine the contributions of gender in learning readiness. This study is based on the cross-sectional survey design and it employed purposive sampling to gather data from 400 students via questionnaire. Then, the data were analyzed employing both descriptive and parametric statistical measures, particularly independent sample t-test. In the meantime, the researcher has incorporated the social capital theory for discussing the results. The derived result reveals that all dimensions (student, school, and family) elucidated the high level of learning readiness among students. Likewise, gender makes significant contributions in learning readiness and its dimensions due to variation in the influences related to the existing social capitals among students. Finally, it is wrapped up that without gender equality, the enhancement of learning readiness is not possible.
Analisis Perilaku Pemilahan Sampah di Kota Surabaya
Authors: Andina Elga 
Published: June 2020

Surabaya is one of the cities with the highest waste generation. Managing waste effectively needs to be started from the source by waste sorting. This qualitative research used a study case approach to figure out trash sorting behavior in Surabaya. Data are gathered from an interview with Surabaya’s Department of Cleanliness and Green Open Space, Non-Government Agency related to waste, and Surabaya’s Main Waste Bank. Observation is conducted in Pusat Daur Ulang Jambangan, city parks and boulevards to see how people sort the waste in public areas. Even though Surabaya has a world-recognized hygiene program, it doesn't necessarily make its people care about cleanliness and sorting waste. The knowledge doesn’t always lead to behavior. Waste sorting behavior is still related to the physical and social conditions of the community (external factors). Thus, to develop the sorting behavior requires three strategies: (1) strengthening policy; (2) providing ergonomic facilities; and (3) community involvement in changing behavior.AbtrakSurabaya merupakan salah satu kota dengan timbulan sampah terbesar. Agar dapat mengelola sampah dengan efektif, maka harus dimulai dari sumber dengan pemilahan sampah. Penelitian kualitatif ini bercorak studi kasus bertujuan mengetahui perilaku pemilahan sampah di Kota Surabaya. Data diperoleh melalui wawancara dengan Dinas Kebersihan dan Ruang Terbuka Hijau Kota Surabaya, LSM Pemerhati Sampah, Bank Sampah Induk Surabaya. Pengamatan dilakukan di Pusat Daur Ulang Jambangan, taman kota dan jalan-jalan untuk mengetahui bagaimana masyarakat memilah sampah di tempat umum. Meskipun Surabaya memiliki program kebersihan yang diakui dunia, tidak serta merta menjadikan masyarakatnya peduli kebersihan dan melakukan pemilahan sampah. Pengetahuan tidak selalu menghasilkan perilaku pemilahan sampah. Perilaku pemilahan sampah masih berkaitan dengan kondisi lingkungan fisik dan sosial masyarakat (faktor eksternal individu). Oleh karena itu, perilaku pemilahan sampah perlu dibentuk dengan 3 strategi: (1) penguatan kebijakan; (2) penyediaan sarana yang ergonomis; dan (3) pelibatan masyarakat dalam mengubah perilaku.
Oxygen-Hafnium-Neodymium Isotope Constraints on the Origin of the Talnakh Ultramafic-Mafic Intrusion (Norilsk Province, Russia)
Authors: Malitch Kreshimir N. , Belousova Elena A. , Griffin William L. , Martin Laure , Badanina Inna Yu. , Sluzhenikin Sergey F. 
Published: June 2020

Abstract
The ultramafic-mafic Talnakh intrusion in the Norilsk province (Russia) hosts one of the world’s major platinum group element (PGE)-Cu-Ni sulfide deposits. This study employed a multitechnique approach, including in situ Hf-O isotope analyses of zircon combined with whole-rock Nd isotope data, in order to gain new insights into genesis of the Talnakh economic intrusion. Zircons from gabbrodiorite, gabbroic rocks of the layered series, and ultramafic rocks have similar mantle-like mean δ18O values (5.39 ± 0.49‰, n = 27; 5.64 ± 0.48‰, n = 34; and 5.28 ± 0.34‰, n = 7, respectively), consistent with a mantle-derived origin for the primary magma(s) parental to the Talnakh intrusion. In contrast, a sulfide-bearing taxitic-textured troctolite from the basal part of intrusion has high δ18O (mean of 6.50‰, n = 3), indicating the possible involvement of a crustal component during the formation of sulfide-bearing taxitic-textured rocks. The Hf isotope compositions of zircon from different rocks of the Talnakh intrusion show significant variations, with ɛHf(t) values ranging from –3.2 to 9.8 for gabbrodiorite, from –4.3 to 11.6 for unmineralized layered-sequence gabbroic rocks, from 2.3 to 12 for mineralized ultramafic rocks, and from –3.5 to 8.8 for mineralized taxitic-textured rocks at the base of the intrusion. The significant range in the initial 176Hf/177Hf values is ascribed to interaction of distinct magma sources during formation of the Talnakh intrusion. These include (1) a juvenile source equivalent to the depleted mantle, (2) a subcontinental lithospheric source, and (3) a minor crustal component. Initial whole-rock Nd isotope compositions of the mineralized taxitic-textured rocks from the base of the intrusion (mean ɛNd(t) = –1.5 ± 1.8) differ from the other rocks, which have relatively restricted ranges in initial ɛNd (mean ɛNd = 0.9 ± 0.2). The major set of ɛNd values around 1.0 at Talnakh is attributed to limited crustal contamination, presumably in deep magma chambers, whereas the smaller set of negative ɛNd values in taxitic-textured rocks is consistent with greater involvement of a crustal component and reflects an interaction with the wall rocks during emplacement.
Human anatomy and clinical nursing practice
Authors: Ratero Lilian Rubinho , André Júlio César , Santos Emerson Roberto dos , Castiglioni Lilian , Poletti Nádia Antônia Aparecida , Sasso Leonila Santos de Almeida , Bertolin Daniela Comelis 
Published: June 2020

Introduction and Objective: Human anatomy is an essential component of the undergraduate nursing curriculum for learning the specific disciplines which deal with clinical practice. Anatomical knowledge provides assurance for the practice of clinical assessment and invasive procedures of legal competence of nurses. The aim of the study was to analyze the correlation of the content taught in the discipline Human Anatomy with the clinical practice of undergraduate nursing students in the discipline Semiology and Semiotics in Nursing and The Care Process, as well as their assurance to start it.Methods: Quantitative descriptive study with the application of an online questionnaire to 66 undergraduate nursing students at a public education institution in the interior of São Paulo. Data analysis by number of occurrences and Chi-square test.Results: There was partial agreement about the interdisciplinarity between human anatomy and disciplines of clinical nursing practice. The students agreed to be partially assured about the procedures to start the semiological practice of different devices and to perform nursing procedures. The predominance of the superficial approach to content related to the clinical practice of the disciplines Semiology and Semiotics in Nursing and The Care Process was predominant.Conclusions: The teaching of human anatomy, along the lines offered, maintains an unsatisfactory correlation with clinical practice due to the students’ experience, interfering with learning, acting in clinical teaching and professional training.
Convênios e Contratos de Repasse do Governo Federal: Uma Análise Comparativa das Aplicações FiscalizaBR e SISCONV
Authors: Silva Álisson Vilaça , Oliveira Paulo Henrique Lima , Costa Heitor Heitor Augustus Xavier , Parreira Júnior Paulo Afonso 
Published: June 2020

O incentivo do governo federal brasileiro à transparência dos dados públicos e ao controle social se tornou cada vez mais evidente após a instauração da Lei de Acesso à Informação. Devido a essa lei, novas ferramentas de busca e novas formas de visualizações têm sido propostas para que o cidadão possa realizar o controle social de maneira eficiente. Este trabalho apresenta a aplicação móvel FiscalizaBR, cujo objetivo é facilitar o acesso, a busca e a visualização das informações sobre contratos e convênios do Governo Brasileiro. Por meio de estudo experimental, constatou-se que a aplicação proposta apresentou valores para efetividade e eficiência maiores do que os de outra aplicação existente com a mesma finalidade.
Internalização de novos membros em equipes de desenvolvimento de software: uma versão detalhada
Authors: Moser Patrícia Cristina , Araújo Joelson Isidro da Silva 
Published: June 2020

Contexto: A internalização de novos membros em equipes de desenvolvimento de software ocasiona mudanças nas organizações e precisa ser estudada. Objetivo: Investigar os benefícios e limitações da internalização em equipes de desenvolvimento de software. Método: Foi realizado um estudo qualitativo em uma startup onde a internalização foi observada recentemente. Os dados foram analisados ​​utilizando técnicas de codificação qualitativa e os resultados foram verificados e validados com os participantes por meio da verificação de membros. Resultados: Percebeu-se que a internalização sofre forte influência da cultura da empresa estudada e do acolhimento e integração do time. Conclusão: A internalização de novos membros gera uma maior sinergia, que ajuda fortemente no objetivo dos resultados organizacionais.
Åben skole i form af nationale fagkonkurrencer
Authors: Christensen Jacob 
Published: June 2020

I nationale fagkonkurrencer har skoleelever mulighed for at dyste i fag, og ofte kobles der undervisningsforløb hertil. Erhvervslivets fagpersoner inviteres ind i undervisningsforløbene, hvilket kan kombineres med, at elever besøger arbejdspladser der beskæftiger sig med fagkonkurrencens emne. Undervisningsformen eksisterede før introduktionen af åben skole, men flere fagkonkurrencer formidles som én måde, hvorpå åben skole kan praktiseres. Denne artikel præsenterer et kvalitativt studie af en fagkonkurrence i faget madkundskab. Der fremskrives perspektiver på, hvordan åben skole forstås i denne sammenhæng, samt hvilke pædagogiske muligheder og udfordringer undervisningsformen implicerer. Der argumenteres for, at erhvervslivets arbejdsformer afspejles i kombination med en rammesætning, hvor elever kan dygtiggøre sig i særlig høj grad. Men bestemte typer af elever og lærere beneficerer af fagkonkurrencer, og pædagogikken risikerer at reproducere uhensigtsmæssige strukturer i skolen. Yderligere risikeres, at præstation i konkurrencen overskygger elevers faglige motivation og læring, samt at læreres didaktiske refleksioner ensidigt fokuseres på præstation.
Tuberculosis Case Finding Practice: The Intention of Cadres in East Nusa Tenggara, Indonesia
Authors: Lepuen Agnes Pude , Ayuningsih Bratajaya Cicilia Nony , Rasmada Sada 
Published: June 2020

Tuberculosis (TB) is a difficult health problem to overcome. Active case finding is an important step in managing this infectious disease. However, the prevalence of TB case finding among cadres at the community level is low because of the stigma attached to TB, difficulty in geographical coverage, low public awareness, and social economic barriers. In addition, the empowerment and intention of cadres to perform community-based TB case finding are not optimal yet. This cross-sectional study aimed to determine the intention of TB case finding among 162 public health cadres at one district. Convenient sampling technique was employed in this study. Relationship analyses were performed using Chi-Square test. Results suggested that three factors, namely, attitude, subjective norm, and perceived behavior control influenced the intention to practice TB case finding among cadres. Public health care providers must encourage cadres to practice active TB case finding and understand the benefits and burdens encountered by cadres during TB case finding. Abstrak Praktik Penemuan Kasus Tubercolusis: Niat Kader di Nusa Tenggara Timur, Indonesia. Tuberculosis (TB) masih menjadi masalah kesehatan yang sulit diatasi. Penemuan kasus TB secara aktif merupakan langkah awal yang menjadi kunci keberhasilan dalam penanganan kasus TB, namun angka penemuan kasus TB masih rendah. Kader belum dapat melakukan pendeteksian dini kasus TB secara optimal. Selain itu sebagai penemu kasus TB di masyarakat, kader memiliki berbagai tantangan dalam upaya menemukan kasus TB, salah satunya adalah niat untuk menemukan kasus TB mengingat banyak stigma yang muncul terkait penyakit TB, keadaan geografi yang sulit dijangkau, rendahnya kesadaran masyarakat, dan kendala biaya. Tujuan dari penelitian ini adalah untuk mengetahui intensi atau niat kader dalam menemukan kasus TB. Penelitian ini menggunakan desain cross sectional dengan melibatkan 162 kader kesehatan di sebuah kecamatan. Metode pengambilan sampel menggunakan convenient sampling. Analisa hubungan menggunakan uji statistik Chi Square. Hasil penelitian menunjukkan ketiga faktor yaitu sikap, norma subjektif, dan kendali perilaku yang dirasakan memiliki hubungan dengan intensi dalam menemukan kasus TB. Maka dapat disimpulkan, dukungan tenaga kesehatan sangat penting dalam meningkatkan praktik penemuan kasus TB dan penting untuk memperhatikan manfaat dan tantangan yang ditemui oleh kader dalam menemukan kasus TB. Kata Kunci: kader kesehatan, kontrol kendali yang dirasakan, niat, norma subjektif, sikap, penemuan kasus Tuberculosis
Burn Injury Induces Intestinal Inflammatory Response Mediated by Th17 in Burn-Primed Endotoxemic Mice
Authors: Sekine Kazuhiko , Shibusawa Takayuki , Fujishima Seitaro , Aikawa Naoki , Sasaki Junichi 
Published: June 2020

Objective
This study aimed to elucidate the mechanism underlying the susceptibility to infection-related acute lung injury by focusing on the role of gut mucosal T-helper (Th) 17 cells that preferentially produce IL-17 with probiotics in a burn-primed endotoxemic mice model.

Methods
Mice were subjected to a 15% total body surface area third-degree burn. Survival from lethal lipopolysaccharide (LPS) administration (3 mg/kg) on 11th day post-burn was assessed in mice fed by chow with or without 1.2% Lactobacillus powder after burn injury. Lamina propria mononuclear cells were enzymatically isolated from the ileum removed on 11th day post-burn and incubated along with 1 μg/mL LPS or 10 μg/mL anti-CD3 antibody for 24 hours; subsequently, the following 7 cytokines were analyzed in the supernatant: IFN-γ, TNF-α, IL-2, IL-4, IL-6, IL-10, and IL-17.

Results
Lactobacillus treatment post-burn injury markedly improved survival after lethal endotoxemia in burn-primed mice (64.3% versus 21.4%, P = 0.03). The production of proinflammatory cytokines such as TNF-α, IL-6, and IL-17 by lamina propria mononuclear T-lymphocytes and macrophages including Th17 response was augmented by burn injury but decreased with Lactobacillus treatment after burn injury.

Conclusions
Th17- and Th17-mediated inflammatory responses in the gut mucosa may play a vital role, which could be attenuated by Lactobacillus treatment, in survival of lethal endotoxemia in burn-primed mice.
Biomedical Micro‐/Nanomotors: From Overcoming Biological Barriers to In Vivo Imaging
Authors: Gao Changyong , Wang Yong , Ye Zihan , Lin Zhihua , Ma Xing , He Qiang 
Published: June 2020

AbstractSelf‐propelled micro‐ and nanomotors (MNMs) have shown great potential for applications in the biomedical field, such as active targeted delivery, detoxification, minimally invasive diagnostics, and nanosurgery, owing to their tiny size, autonomous motion, and navigation capacities. To enter the clinic, biomedical MNMs request the biodegradability of their manufacturing materials, the biocompatibility of chemical fuels or externally physical fields, the capability of overcoming various biological barriers (e.g., biofouling, blood flow, blood–brain barrier, cell membrane), and the in vivo visual positioning for autonomous navigation. Herein, the recent advances of synthetic MNMs in overcoming biological barriers and in vivo motion‐tracking imaging techniques are highlighted. The challenges and future research priorities are also addressed. With continued attention and innovation, it is believed that, in the future, biomedical MNMs will pave the way to improve the targeted drug delivery efficiency.
Wood‐Derived Functional Polymeric Materials
Authors: Wang Jifu , Zhang Daihui , Chu Fuxiang 
Published: June 2020

AbstractIn recent years, tremendous efforts have been dedicated to developing wood‐derived functional polymeric materials due to their distinctive properties, including environmental friendliness, renewability, and biodegradability. Thus, the uniqueness of the main components in wood (cellulose and lignin) has attracted enormous interest for both fundamental research and practical applications. Herein, the emerging field of wood‐derived functional polymeric materials fabricated by means of macromolecular engineering is reviewed, covering the basic structures and properties of the main components, the design principle to utilize these main components, and the resulting wood‐derived functional polymeric materials in terms of elastomers, hydrogels, aerogels, and nanoparticles. In detail, the natural features of wood components and their significant roles in the fabrication of materials are emphasized. Furthermore, the utilization of controlled/living polymerization, click chemistry, dynamic bonds chemistry, etc., for the modification is specifically discussed from the perspective of molecular design, together with their sequential assembly into different morphologies. The functionalities of wood‐derived polymeric materials are mainly focused on self‐healing and shape‐memory abilities, adsorption, conduction, etc. Finally, the main challenges of wood‐derived functional polymeric materials fabricated by macromolecular engineering are presented, as well as the potential solutions or directions to develop green and scalable wood‐derived functional polymeric materials.
Effects of Nanoscale Roughness on the Lubricious Behavior of an Ionic Liquid
Authors: Nalam Prathima C. , Sheehan Alexis , Han Mengwei , Espinosa‐Marzal Rosa M. 
Published: June 2020

AbstractWhile many mechanistic studies have focused on the lubricious properties of ionic liquids (ILs) on ideally smooth surfaces, little is known about the mechanisms by which ILs lubricate contacts with nanoscale roughness. Here, substrates with controlled density of nanoparticles are prepared to examine the influence of nanoscale roughness on the lubrication by 1‐hexyl‐3‐methyl imidazolium bis(trifluoromethylsulfonyl)imide. Atomic force microscopy is employed to investigate adhesion, hydrodynamic slip, and friction at the lubricated contact as a function of surface topography for the first time. This study reveals that nanoscale roughness has a significant influence on the slip along the surface and leads to a maximum slip length on the substrates with intermediate nanoparticle density. This coincides with the minimum friction coefficient at sufficiently small contact stresses, likely due to the lower resistance of the IL film to shear. However, at the higher pressures applied with a sharp tip, friction increases with nanoparticle density, indicating that the IL is not able to alleviate the increased dissipation due to roughness. The results of this work point toward a complex influence of the surface topology on friction. This study can help design ILs and nanopatterned substrates for tribological applications and nano‐ and microfluidics.