bluuebunny/crossref_metadata_2025_split huggingface dataset:
KARAKTERISTIK FISIOLOGI ISOLAT Pleurotus spp.
Authors: ACHMAD ACHMAD , HERLIYANA ELIS NINA , YURTI OSICA ASNO FERINA , HIDAYAT ANANG PRANOTO 
Published: June 2020

<p>ABSTRAK</p><p>Studi in vitro tentang karakteristik fungi isolat Pleurotus spp. telahdilaksanakan di Bogor dari bulan Juli sampai Agustus 2004. Penelitianmenggunakan rancangan faktorial dalam rancangan acak lengkap danbertujuan untuk mempelajari pengaruh media, temperatur inkubasi dan pHmedia terhadap 6 isolat Pleurotus sp. Karakter lain yang juga dipelajariadalah kemampuan untuk mengoksidasi asam tanat dan asam galat dalammedia agar. Hasil penelitian menunjukkan bahwa Pleurotus isolat sp.6dan sp.8 tumbuh baik pada media MPA, isolat Pleurotus sp.1, 3 dan 4pada media MEA dan isolate sp.2 pada media PDA. Kecuali isolat sp.8,isolat lainnya tidak dapat tumbuh pada temperatur 10 dan 35 o C. Pertum-buhan isolat sp.8 terbaik dibandingkan isolat lainnya pada semuatemperatur. Diameter koloni isolat lainnya hanya mencapai 0,2 – 2,33 cm.Pertumbuhan isolat sp.8 juga terbaik pada semua pH media diikuti isolatsp.6 kemudian isolat sp.4. Semua isolat menunjukkan reaksi oksidasipositif pada agar asam tanat dan asam galat yang ditunjukkan oleh warnacoklat pada media yang melingkari koloni.</p><p>Kata kunci : Pleurotus spp, media, temperature, pH, oksidasi, asam tanat,asam galat</p><p>ABSTRACT</p><p>Physiological Characteristics of Pleurotus spp. IsolatesPhysiological characteristics of some Pleurotus sp. isolates werestudied in vitro, from July until August 2004 in Bogor. Experiments tostudy the effect of kind of media, temperature of incubation room, and pHof medium on six isolates of Pleurotus sp. were arranged in factorialrandomized complete design and replicated three times with colony in apetri dish as experimental units. Another physiological character studiedwas the ability to oxidize tannic and gallic acids in agar medium. Resultsshowed that isolates Pleurotus sp.6 and -8 grew better in MPA medium,Pleurotus sp.1, -3, and -4 in MEA, and Pleurotus sp.2 in PDA. ExceptPleurotus sp.8, other isolates could not grow in incubation roomtemperature of 10 and 35 o C. The growth of Pleurotus sp.8 was the bestamong the isolates in all temperature levels. Other isolates grew poorly in20 and 29o C with diameter range was 0.2 – 2.33 cm. The growth ofPleurotus sp.8 was also the best in all pH medium levels, followed byPleurotus sp.6, and then Pleurotus sp.4. All isolates showed positiveoxidative reaction on tannic and gallic acid agar indicated by brown colorof the medium around the colony.</p><p>Key words: Pleurotus spp., medium, temperature, pH, oxidation, tannicacid, gallic acid</p>
Assessment of dynamic balancing responses following perturbations during slow walking in relation to clinical outcome measures for high-functioning post-stroke subjects
Authors: Zadravec Matjaž , Olenšek Andrej , Rudolf Marko , Bizovičar Nataša , Goljar Nika , Matjacic Zlatko 
Published: June 2020

Abstract
Background: Generating appropriate balancing reactions in response to unexpected loss of balance during walking is important to prevent falls. The purpose of this study was to assess dynamic balancing responses following pushes to the pelvis in groups of post-stroke and healthy subjects. Methods: Forty-one post-stroke subjects and forty-three healthy subjects participated in the study. Dynamic balancing responses to perturbations triggered at heel strike of the left or right leg, directed in the forward, backward, inward and outward directions during slow treadmill walking were assessed. Responses of the healthy group provided reference values used to classify responses of the post-stroke group into two subgroups; one within the reference responses (“inside” subgroup) and the other that falls out (“outside” subgroup). A battery of selected clinical outcome measures (6-Minute Walk Test, 10-Meter Walk Test, Timed-Up-and-Go test, Four Square Step Test, Functional Gait Assessment, Functional Independence Measure and One-legged stance test) was additionally assessed in the post-stroke group. Results: The “inside” subgroup of post-stroke subjects was able to appropriately modulate centre-of-pressure and ground-reaction-force both under the impaired and non-impaired leg in response to perturbations. The “outside” subgroup of post-stroke subjects showed limited modulation of centre-of-pressure and ground-reaction-force under the impaired leg; instead stepping strategy was used in which the non-impaired leg was placed such as to make a longer step (forward perturbation), to make a shorter step (backward perturbation) or to make a cross-step (outward perturbation). Consequently, peak centre-of-mass displacements following perturbations were significantly higher in the “outside” subgroup compared to the “inside” subgroup. Responses in both subgroups following inward perturbations did not differ. Majority of clinical outcome measures moderately correlated with the peak centre-of-mass displacements for forward perturbations and exhibited weak correlations for other perturbation directions.Conclusions: Substantial number of post-stroke subjects, that were considered to be independent walkers, have reduced capabilities to execute appropriate balancing responses following perturbations commencing on the hemiparetic leg and may thus benefit from perturbation-based training. Timed-Up-and-Go and Functional Independence Measure tests may provide an indication on the abilities of each subject to counteract unexpected loss of balance. However, a reliable assessment should be done through perturbation-based measures.
Eucommia Ulmoides Flavones as Potential Alternatives to Antibiotic Growth Promoters in a Low-protein Diet Improve Growth Performance and Intestinal Health in Weaning Piglets
Authors: Yuan Daixiu , Wang Jing , Xiao Dingfu , Li Jiefeng , Liu Yanhong , Tan Bie 
Published: June 2020

Abstract

Background: Eucommia ulmoides flavones (EUF), have been demonstrated to attenuate the inflammation and oxidative stress of piglets. This study was designed to test whether EUF could be used as an antibiotic growth promoter alternative to support growth performance and maintain intestinal health in weanling-piglets. Weaned-piglets (n = 480) were assigned into 3 groups and fed with a low-protein basal diet (NC), or supplementation with antibiotics (75 mg/kg quinocetone, 20 mg/kg virginomycin and 50 mg/kg aureomycin, PC) or 0.01% EUF (EUF). Blood, intestinal contents and intestine were collected on d-15 and d-35 after weaning, respectively. Results: The results showed that the body weight on d-35, average daily gain and gain : feed ratio from d 15 to d 35 and d 0 to d 35 in piglets of PC and EUF treatments were higher than (P < 0.05) these in NC treatment, whereas the initial body weight among 3 treatments were consistent. PC and EUF treatments decreased (P < 0.05) the diarrhea index of weanling piglets. Dietary supplementation of EUF significantly enhanced (P < 0.05) the serum concentrations of total protein, alanine transaminase and the immunoglobulin G on d-15 of piglets compared to piglets in NC treatment. EUF supplementation increased (P < 0.05) the jejunal villus height : crypt depth ratio, jejunal and ileal villus height and the population of ileal lactic acid bacteria on d-15 but remarkable decreased (P < 0.05) the population of ileal coliform bacteria on d-15 and d-35. Conclusion: These findings indicated the EUF, as the potential alternative to in-feed antibiotic growth promoter, could improve growth performance and intestinal morphology, decreasing colonization of coliform bacteria and diarrhea index in weanling piglets.
Transient Vision Restoration Training Using a Unique Virtual Reality Platform in Glaucoma
Authors: Li Xiao Xia , Chu Hang , Yan Li , Lu Yan 
Published: June 2020

Abstract
Background

To evaluate the efficacy of transient vision restoration training (tVRT) with an augmented virtual reality platform in glaucoma patients.
Design:
A self-control, prospective study.
Methods

This study recruited subjects with glaucoma. All participants were treated with tVRT which based on an augmented virtual reality platform for 20 minutes. The intraocular pressure (IOP), the best-corrected visual acuity (BCVA), global mean defect (MD) values, global indices mean sensitivity (MS) were evaluated and compared before and after treatment.
Results

While the IOP and BCVA after tVRT did not change obviously compared to baseline. However, the global MD significantly reduced, consistently the global MS changed better in the treated patients.
Conclusions

The glaucomatous optic neuropathy remains potentially neuroplasticity. And the training based on an augmented virtual reality platform may have a positive impact on vision restoration.
Effect of Hormones (IBA &amp; IAA) on the Propagation of Himalayan Yew in Pakistan: A Conservation Approach
Authors: Iqbal Javaid , Khan Bushra , Khan Sardar , Ghaffar Nasreen , Mian Ishaq Ahmad , Yousaf Nowsher , Ahmad Iftikhar , Manzoor Sadaf 
Published: June 2020

Abstract
Background

Himalayan yew (Taxus wallichiana) is one of the endangered medicinal plants species having great importance due to the presence of anticancer drug Taxol. This metabolite is mainly used for the treatment of ovarian, breast, AIDS-related cancers, and other indications. The study being reported here was conducted for the propagation of Himalayan yew by using two different trials of Indole Butyric acid (IBA) and Indole acetic acid (IAA) hormones treatments (2000–7000 ppm) through stem cuttings (140 each). In the same way, 3840 cuttings were treated with IBA and IAA from November 2016 to November 2017 at Lalku valley, Swat, Khyber Pakhtunkhwa (KP), Pakistan.
Results

The influence of IBA treatment (7000 ppm) showed a survival of 85.22% (average number of roots = 10.4, average length of roots = 15.5 cm, average number of leaves = 92.4 and average number of sprouts = 3.3) while that of IAA treatment (7000 ppm) the survival of 81.11% (average number of roots = 9.1, average length of roots = 14.6 cm, average number of leaves = 84.0 and average number of sprout = 3.0) were more significant followed by 2000–6000 ppm (IBA and IAA). Lowest survival 40–45% (average number of roots = 4.2, average length of roots = 8.0 cm, average number of leaves = 32.2, average number of sprouts = 1.7) was noticed for controlled cuttings.
Conclusion

The present study enhanced the potential of conservation and propagation of T. wallichiana. Hence our study suggests and recommends the application of IBA (7000 ppm) as a better hormone for the conservation and propagation of Himalayan yew.
Imported Mansonella perstans in migrants, Spain
Authors: Puente Sabino , Lago Mar , Subirats Mercedes , Sanz-Esteban Ismael , Arsuaga Marta , Vicente Belen , Alonso-Sardon Montserrat , Belhassen-Garcia Moncef , Muro Antonio 
Published: June 2020

Abstract
Background: Mansonella perstans infection can be considered one of the most neglected tropical infectious diseases. Very few studies have reported on the clinical picture caused by infection with this nematode. Methods: The present study evaluated a large cohort of migrants who have been diagnosed, examined and treated for imported M. perstans infection at a Spanish reference center (Hospital Carlos III Tropical Medicine Unit, Madrid, Spain) over a 19-year period. Results: Five hundred three cases of migrants from tropical and subtropical areas with M. perstans infection were identified. Two hundred sixty-four patients were female (52.5%). The mean age (±SD) was 44.6±18.2 years (range, 16–93 years). The mean time (±SD) between the arrival in Spain and the first consultation was 8.6±18.0 months. The origin of the patients was Equatorial Guinea (97.6%). Regarding the clinical picture, 257 patients were asymptomatic (54.7%) and 228 were symptomatic (45.3%); 190 patients had pruritus (37.8%), 50 (9.9%) had arthralgia, 18 patients had Calabar-like swelling (3.6%), and 15 (3%) had abdominal pain. Four hundred forty-two (87.9%) migrants had hyper-IgE, and 340 (67.6%) had eosinophilia. One hundred ninety-five patients had coinfections with other filarial nematodes (38.8%), and 308 migrants had only M. perstans infection (61.2%). Four hundred thirty-seven cases (86.9%) had been treated with anti-filarial drugs: 292 cases were treated with one anti-filarial drug, and 145 cases were treated with combined anti-filarial therapy. Additionally, 20 (4%) cases received steroids and 38 (7.6%) cases received antihistamines. No statistical association was found between the percentage of side effects to treatment and cases healed (p=0.094). Conclusions: A long series of M. perstans infections is presented in sub-Saharan immigrants whose data indicate that it should be included in the differential diagnosis in patients with pruritus or analytical alterations such as eosinophilia or hyper-IgE presentation, and they also have a high number of coinfections with other microorganisms whose treatment needs to be protocolized.
Quantitative dynamic contrast-enhanced MR imaging can be used to predict the pathologic stages of lingual squamous cell carcinoma
Authors: Guo Na , Zeng Weike , Hu Huijun , Deng Hong , Cheng Ziliang , Yang Zehong , Jiang Shuqi , Duan Xiaohui , Shen Jun 
Published: June 2020

Abstract
Background
To investigate whether quantitative DCE-MRI pharmacokinetic parameters can be used to predict the pathologic stages of lingual squamous cell carcinoma (LSCC).
Methods
For this prospective study, DCE-MRI was performed in participants with LSCC from May 2016 to June 2017. The pharmacokinetic parameters, including Ktrans, Kep, Ve, and Vp, were derived from DCE-MRI by utilizing a two-compartment extended Tofts model and a three-dimensional volume of interest. The postoperative pathologic stage was determined in each patient based on the 8th AJCC cancer staging manual. The quantitative DCE-MRI parameters were compared between stage I-II and stage III-IV lesions. Logistic regression analysis was used to determine independent predictors of tumor stages, followed by receiver operating characteristic (ROC) analysis to evaluate the predictive performance.
Results
The mean Ktrans, Kep and Vp values were significantly lower in stage III-IV lesions compared with stage I-II lesions (P = 0.013, 0.005 and 0.011, respectively). Kep was an independent predictor for the advanced stages as determined by univariate and multivariate logistic analysis. ROC analysis showed that Kep had the highest predictive capability, with a sensitivity of 64.3%, a specificity of 82.6%, a positive predictive value of 81.8%, a negative predictive value of 65.5%, and an accuracy of 72.5%.
Conclusion
The quantitative DCE-MRI parameter Kep can be used as a biomarker for predicting pathologic stages of LSCC.
Hair cortisol- a novel method to explore Prader-Willi syndrome.
Authors: Shukur Hasanain Hamid , de Rijke Yolanda B , Rossum Elisabeth FC van , Hussain-Alkhateeb Laith , Höybye Charlotte 
Published: June 2020

Abstract
Background: Prader-Willi syndrome (PWS) is a multisymptomatic, rare, genetic, neurodevelopmental disorder in adults mainly characterized by hyperphagia, cognitive dysfunction, behavioral problems and risk of morbid obesity. Although endocrine insufficiencies are common, hypocortisolism is rare and knowledge on long-term cortisol concentrations is lacking. The aim of this study was to evaluate long-term cortisol levels in PWS by measurements of hair cortisol. Methods: Twenty-nine adults with PWS, 15 men and 14 women, median age 29 years, median BMI 27 kg/m2, were included. Scalp hair samples were analyzed for cortisol content using liquid-chromatography tandem-mass spectrometry. In addition, a questionnaire on auxology, medication and stress were included. For comparison, 105 age- and sex-matched participants from the population-based Lifelines Cohort study were included as controls. The mean hair cortisol between the groups were compared and associations between BMI and stress were assessed by a generalized linear regression model. Results: In the PWS group large variations in hair cortisol was seen. Mean hair cortisol was 12.8±25.4 pg/mg compared to 3.8±7.3 pg/mg in controls (p=0.001). The linear regression model similarly showed higher cortisol levels in patients with PWS, which remained consistent after adjusting for BMI and stress (p=0.023). Furthermore, hair cortisol increased with BMI (p=0.012) and reported stress (p=0.014). Conclusion: Long-term cortisol concentrations were higher in patients with PWS compared to controls and increased with BMI and stress, suggesting an adequate cortisol response to chronic stress. Hair cortisol demonstrate promising applications in the context of PWS treatment and disease management.
Androgens deficiency impairs thoracic aortic vascular function by promoting smooth muscle cell proliferation via an autophagy dependent manner
Authors: Zhang Na-na , Li Ming , Gao Feng-Bin , Wang Xian-Jin , Dong Fan , Shen Yi-Fan , Zhong Shan , Chen Shan-Wen , Ding Qiang , Zhang Min-Guang 
Published: June 2020

Abstract
Background

Androgens play an essential role in maintaining vascular hemostasis via the regulation of autophagy, but the underlying mechanisms remain unknown. Here we investigated the effects of androgen deficiency in a rat castration model and the effects of androgen treatment in human umbilical vein smooth muscle cells (HUVSMCs). Male Sprague–Dawley rats were divided into two groups: (A) control group, (B) castration group. The vasodilation and vasoconstriction effect of dihydrotestosterone (DHT) were detected on rat thoracic aortic rings. Transmission electron microscopy was used to determine the levels of autophagy. Masson’s trichrome staining, MTT assay and flow cytometry were performed to detect the levels of HUVSMC cell proliferation.
Results

Androgen deficiency led to significantly enhance in the vasoconstriction and reductions in the vasodilatation of rat thoracic aortic rings. Treatment with rapamycin or 3-MA attenuated and enhanced, respectively, the vasorelaxation effect of androgen deficiency. Autophagy was significantly increased in the endothelial cells and SMCs of the thoracic aortic rings of model rats. Moreover, androgen deficiency induced excessive proliferation of SMCs. Treatment of HUVMSCs with high concentrations of DHT increased the percentage of cells in G0/G1 phase and reduced the percentages of cells in the G2 phase and S phase.
Conclusions

It was concluded that vascular injury resulting from androgen deficiency is mediated, in part, by increased autophagy. By promoting SMCs proliferation, androgen deficiency results in alteration of the aortic structure and aggravates vascular injury. Androgen treatment can inhibit the excessive proliferation of SMCs.
WRN modulates mRNA translation by influencing mRNA export from the nucleus through its interaction with the export receptor NXF1 in HeLa cancer cell
Authors: Iglesias-Pedraz Juan Manuel , Jara Diego Matia Fossatti , Felice Valeria Del Carmen Valle-Riestra , Visalaya Sergio Rafael Cruz , Felix Jose Antonio Ayala , Comai Lucio 
Published: June 2020

Abstract
BackgroundThe Werner syndrome protein (WRN) belongs to the RecQ family of helicases and its loss of function results in the premature aging disease Werner syndrome (WS). We previously demonstrated that an early cellular change induced by WRN depletion is a posttranscriptional decrease in the levels of enzymes involved in metabolic pathways that control macromolecular synthesis and protect from oxidative stress. This metabolic shift is tolerated by normal cells but causes mitochondria dysfunction and acute oxidative stress in rapidly growing cancer cells, thereby suppressing their proliferation.Results To identify the mechanism underlying this metabolic shift, we examined global protein synthesis and mRNA nucleocytoplasmic distribution after WRN knockdown. We determined that WRN depletion in HeLa cells attenuates global protein synthesis without affecting the level of key components of the mRNA export machinery. We further observed that WRN depletion affects the nuclear export of mRNAs and demonstrated that WRN directly interacts with mRNA and the mRNA export receptor Nuclear Export Factor 1 (NXF1).Conclusions Our findings suggest that WRN influences the export of mRNAs from the nucleus through its interaction with the NXF1 export receptor thereby affecting cellular proteostasis. In summary, we identified a new partner and a novel function of WRN, which is especially important for the proliferation of cancer cells.
A cost analysis of options for schistosomiasis control MDA programs targeting children aged five years and below in uMkhanyakude District of KwaZulu-Natal Province, South Africa
Authors: Ncube Mhlengi Vella , Chimbari Moses John 
Published: June 2020

Abstract
BackgroundSchistosomiasis negatively impacts early childhood development. Inclusion of children aged five years and below in mass drug administration (MDA) programs for controlling schistosomiasis could improve early childhood development in communities where the disease is endemic. We estimated the projected cost of implementing a schistosomiasis control MDA program for children aged five years and below in the uMkhanyakude district of South Africa.MethodWe calculated the cost of implementing a schistosomiasis MDA program targeting children aged five years and below using an economies of scaled based cost function. We further compared different labor composition simulations to determine the most affordable and available human resources to implement the program. We also explored programs to which the MDA program could be integrated; and estimated what the costs for would be. Moreover, we simulated cost-effectiveness and determined the cost drivers for each simulation considered.ResultsA ward-based outreach team (WBOT) for implementing a schistosomiasis MDA program targeting children 5 years old and below was the best labor composition option. The simulations conducted indicated that treating children in batches of 2500 using the WBOT team approach could reduce the cost of treatment by 53% compared to treating the children on batches of 500. Integrating a schistosomiasis MDA targeting children aged 5 years and below with the immunization program was estimated to cost 3% less than integration with the deworming and Vitamin A supplementation program indicating that the former option is more cost-effective. Praziquantel, the drug that is used to treat schistosomiasis contributed 59% of the total cost for such a program.Conclusion.We estimated that US$4.3 million would be needed to implement a cost effective MDA program targeting children 5 years old and blow over 3 years in uMkhanyakude district.