bluuebunny/crossref_metadata_2025_split huggingface dataset:
Repairing effects of glucosamine sulfate in combination with etoricoxib on articular cartilages of patients with knee osteoarthritis
Authors: Sun Yong , Wang Changde , Gong Chunzhu 
Published: March 2020

Abstract
Purpose: To evaluate the repairing effects of glucosamine sulfate combined with etoricoxib on articular cartilages of patients with knee osteoarthritis (KOA). Methods: A total of 106 KOA patients were randomly divided into control (n=40) and experimental groups (n=66), and treated with etoricoxib alone and glucosamine sulfate plus etoricoxib respectively. Changes in WOMAC score and clinical efficacy were observed. The synovial fluid was extracted. Bone metabolism indices, growth factors, inflammatory factors, matrix metalloproteinases (MMPs) and NO-induced apoptosis-related factors were measured by ELISA. JNK and Wnt5a mRNA levels were determined using RT-PCR. Results: After treatment, the total WOMAC scores of both groups significantly declined (P<0.05), being lower in experimental group. The total effective rate of experimental group was higher (P<0.05). BGP and OPG levels rose, especially in experimental group (P<0.05). CTX-II, COMP and RANKL levels decreased, particularly in experimental group (P<0.05). TGF-β, IGF-1 and FGF-2 levels increased, especially in experimental group (P<0.05). Both groups, particularly experimental group, had decreased levels of IL-1β, IL-17, IL-18, TNF-α, MMP-3, MMP-9 and MMP-13 (P<0.05). JNK and Wnt5a mRNA levels of both groups dropped, which were lower in experimental group (P<0.05). NO and LPO levels reduced, being lower in experimental group. SOD level rose, especially in experimental group (P<0.05). Conclusion: Glucosamine sulfate plus etoricoxib can repair the articular cartilages of KOA patients. Probably, JNK and Wnt5a are down-regulated to inhibit the secretion of MMPs through lowering the levels of inflammatory factors, thereby delaying cartilage matrix degradation. NO-induced chondrocyte apoptosis may be suppressed via the SOD pathway.
Desquamation in Kawasaki Disease and its Relationship with Coronary Artery Abnormalities
Authors: YAN JIAHUEI , Lo Wan-Shan , Guo Mindy Ming-Huey , Huang Ying-Hsien , Chang Ling-Sai , Kuo Ho-Chang 
Published: March 2020

Abstract
Background

Desquamation of hands and feet is a common characteristic of Kawasaki disease (KD), and occurs in 68-98% of patients diagnosed with KD. In this study, we analyzed patients’ varying desquamation levels in their hands or feet, in correlation with clinical presentation, to assess the relationship.

Method

We retrospectively reviewed children with KD who had follow-up visits in Kaohsiung Chang Gung Memorial Hospital’s pediatric outpatient department (OPD) during 2018 and 2019. We analyzed their age, laboratory data before intravenous immunoglobulin (IVIG) treatment, and coronary artery abnormalities (CAA) based on the desquamation level of their hands and feet.

Results

We enrolled a total 112 patients in the study, and 92 patients (82.1%) had hand or feet desquamation. We found the hands’ high-grade desquamation was positively associated with age and segmented neutrophil percentage (p=0.047 and 0.029, respectively) but negatively associated with lymphocyte and monocyte percentage (p=0.03 and 0.006, respectively). Meanwhile, the feet’s high-grade desquamation was positively associated with total white blood cell count (p=0.033). Furthermore, we found that high-grade hand desquamation had less probability of CAA formation compared with that of a low grade (7.1% vs. 40.8%, p=0.016). We observed no significant difference in IVIG resistance between the groups.

Conclusion

This report is the first to demonstrate that the desquamation level of hands or feet in Kawasaki disease is associated with different coronary artery abnormalities and laboratory findings. Physicians should be cautious of CAA formation, particularly in patients with low-grade hand peeling.
Expression and prognostic roles PRDXs gene family in hepatocellular carcinoma
Authors: Xu Mingxing , Xu Jianliang , Zhu Dun , Su Rishun , Zhuang Baoding , Xu Ruiyun , Li Lingli , Chen Shuxian , Ling Yunbiao 
Published: March 2020

Abstract
Background: As the second leading cause of cancer-related death in the world, the therapeutic effect and 5-year overall survival of hepatocellular carcinoma (HCC) are not optimistic. Previous researches indicated that the disorder of PRDXs was related to the occurrence and development of cancer.Methods: In this study, analyzing through CCLE, UALCAN, HCCDB and Human Protein Atlas database databases, PRDXs not only existed in various tumor cell lines, but also had maladjusted expression on mRNA and protein levels in HCC tissues. Besides, the correlations between mRNA as well as methylation levels of PRDXs and clinical characterization were evaluated using UALCAN.Results: Kaplan-Meier Plotter demonstrated that high expression of PRDX1 or low expression of PRDX2/3 suggested poor prognosis for HCC patients. The mutation frequency, type and biological interaction network of PRDXs were obtained from c-Bioportal. By LinkedOmics, PRDXs related differential expressions genes were obtained, which were involved in several canonical pathways and certain amino acid metabolism, as well as miRNAs targeting PRDXs, some of which may effect on the progression of HCC.Conclusions: In conclusion, the disordered expression of PRDXs was correlated with prognosis of HCC patients analyzing by online tools, suggesting that PRDXs could be new molecular targets for HCC treatment and prognosis prediction.
Sociodemographic inequalities in utilizing maternal healthcare services in Cambodia
Authors: Zhou Donghua , Zhou Zhonghe , Yang Cheng , Ji Lu , Ghose Bishwajit , Tang Shangfeng 
Published: March 2020

Abstract
Background Cambodia is a Southeast Asian country and has one the highest rates of maternal and child mortality with inadequate use of maternal healthcare services in the region. The present study aimed to analyse the progress made in terms of using maternal healthcare services since 2000.Methods Two rounds of Demographic and Health Surveys (DHS 2000 and DHS 2014) were used in the study. Sample population consisted 11,961 women aged between 15-49 years. The outcome measures were: Timing of first ANC attendance, adequacy of ANC attendance, place of delivery and postnatal checkup. WHO guidelines were used to set the cut-off/define these measures.Results Findings indicated that the overall prevalence of making the first ANC visit in the first trimester was 64.19% [95%CI=62.22,66.11], and that of having at least four ANC visits was 43.80% [95%CI=41.89,45.73]. Prevalence of health facility delivery was 48.76% [46.62,50.90] and that of postnatal checkup was 71.14% [95%CI=69.21,73.01]. Between 2000 and 2014, the percentage of timely and adequate use of ANC increased by respectively 61.8% and 65.3%, while that of health facility delivery and postnatal care increased by respectively 74.5% and 43.9%. Important demographic, socioeconomic and geographic disparities were observed in the utilization of ANC, health facility delivery and postnatal care services. Urban residency, having better educational status, white collar job, access to electronic media showed positive association, whereas higher parity (having >2 children) and unwanted pregnancy showed negative association with the use of maternal healthcare services. Having at least four ANC visits was associated with significantly increased higher odds of using health facility delivery and postnatal care.Conclusion There has a been a remarkable increase in the prevalence of women who are using the maternal healthcare services since 2000. Although the data are cross-sectional that precludes assuming any causal relationship, the current findings provide important insights regarding the pattern of sociodemographic inequalities in the use of maternal healthcare services in Cambodia.
Measuring parent proxy-reported quality of life of 11 rare diseases in children in Zhejiang, China
Authors: Gao Qisheng , Wang Shanshan , Ren Jianping , Wen Xin 
Published: March 2020

Abstract
Background It is increasingly important to measure the health-related quality of life (HRQoL) of rare disease in children and adolescents in recent decades. Much attention has been paid to investigate the HROoL of one specific rare disease by self-report in previous studies. This study aimed to evaluate and compare the HROoL of 11 rare diseases in Chinese children by parent proxy-report, to explore the factors associated with HROoL of patients and to know the problems of most concern. Methods A total of 651 children aged from 2 to 18 were enrolled from the Children’s Hospital Affiliated Zhejiang University in 2018. Their parents completed the parent proxy-reports version of the Pediatric Quality of Life Inventory TM 4.0 (PedsQL TM ). Independent-samples t -test, one-way ANOVA or Kruskal-Wallis H test was used to compare HROoL scores between groups. Multilevel linear regression models with random intercepts was applied to analyze the relationship between socioeconomic variables and both the total score and sub-domain scores. Results The total PedsQL scores of Patent ductus arteriosus, Infantile agranulocytosis, Autoimmune thrombocytopenia, Polysyndactyly, Hirschsprung disease, Cleft lip and palate, Tetralogy of fallot, Myasthenia gravis, Guillain-barre syndrome, Glycogen storage disease and Langerhans cell histiocytosis children were 79.65±5.46, 95.88±3.48, 71.39±3.27, 91.77±6.35, 76.18±6.92, 96.33±4.22, 77.85±8.90, 95.99±3.31, 85.77±4.56, 82.97±4.13 and 77.6±5.15, respectively. Age was significantly associated with physical functioning, school functioning and psychosocial health score. Gender and household registration place were significantly associated with overall score. The most urgent desire of patients was reducing the overall medical costs. Conclusions These data show that Patent ductus arteriosus scores lowest in physical functioning, Autoimmune thrombocytopenia (ITP) ranks the lowest in emotional functioning score, social functioning score, school functioning score, psychosocial health score and total score. Incentive policies should be further adopted to improve orphan drug availability and reduce the financial burden of rare diseases.
Semaphorin 4D induces an imbalance of Th17/Treg cells by activating the aryl hydrocarbon receptor in ankylosing spodylitis
Authors: Xie Jianmin , Zitao Wang , Jieruo Gu 
Published: March 2020

Abstract
Background: Semaphorin 4D (Sema4D) is constitutively expressed on T cells and osteoclasts, and regulates T cell proliferation and bone remodeling. In addition, several studies have shown that Sema4D is involved in the pathogenesis of autoimmunity. We undertook this study to investigate the mechanism by which Sema4D affects the pathogenic progress of ankylosing spondylitis (AS). Methods: Soluble Sema4D (sSema4D) levels in serum were analyzed by enzyme-linked immunosorbent assay. The cell surface levels and transcripts of Sema4D were evaluated in CD4+ and CD19+ cells from the AS patients and healthy individuals. The mRNA expression levels were assessed by quantitative polymerase chain reaction (qPCR). The proportions of Treg cells and IL-17-producing T-cells (Th17 cells) differentiated from CD4+ T cells were analyzed by flow cytometric analysis. The aryl hydrocarbon receptor (AhR) agonistic effect of Sema4D was detected by analyzing the activation of downstream signaling pathways and target genes using Luciferase and EROD assay.Results: Levels of sSema4D were elevated in both serum from AS patients, and clinical features markers were correlated with serum sSema4D levels. Sema4D facilitated CD4+ T cells proliferation and Th17 cells differentiation and inhibited Treg cells differentiation by enhancing RORγt expression and reducing Foxp3 expression, with increasing expression and secretion of IL-17 and IL-22. It induced the expression and activity of AhR target gene CYP1A1 and XRE reporter activity via interaction with CD72. Conclusions: These findings indicate that Sema4D as a potent activator of T cells in the immune response contributes to the inflammation of AS by inducing imbalance in Th17 and Treg cell populations in an AhR-dependent manner, suggesting it is a crucial participant in AS pathogenesis.
Validation of T-MoCA in the screening of mild cognitive impairment in Chinese patients with atrial fibrillation
Authors: Lai Yiwei , Jiang Chao , Du Xin , Wang Zhiyan , Zhang Jingrui , Liu Xiaobo , Li Jingye , Bai Yu , Xu Baolei , Zhang Weiwei , Bai Rong , Tang Ribo , Liu Nian , Guo Xueyuan , Jiang Chenxi , Li Songnan , Long Deyong , Yu Ronghui , Dong Jianzeng , Sang Cai-hua , Ma Chang-sheng 
Published: March 2020

Abstract
Background: Atrial fibrillation (AF) is associated with high risk of mild cognitive impairment (MCI) and dementia. However, feasible and simple instruments that facilitates regular assessment of cognitive status in AF patients remain underdeveloped. Methods: Cognitive function of 136 AF patients was first evaluated using T-MoCA. Cognitive function of 101 patients was then assessed through in-person interview by physicians who are blinded to telephone interview results, using both Clinical Dementia Rating (CDR) and Mini-Mental Status Evaluation (MMSE). Using CDR=0.5 as a reference standard, the ability of T-MoCA and MMSE to discriminate cognitive dysfunction, stratified by education level, was tested by receiver-operating curve (ROC) analysis. Net reclassification index was calculated for comparison between the performance of T-MoCA and MMSE. Results: Thirty-five MCI patients were identified as MCI using the criteria of CDR=0.5. The areas under the ROC curve of T-MoCA were 0.80 (0.71-0.89), 0.83 (0.71-0.95), and 0.85 (0.64-0.92) for all patients, patients with high educational level, and patients with low education level, respectively. The optimal threshold was achieved at 16/17 with a sensitivity of 85.7% and a specificity of 69.7% in overall patients, 15/16 with a sensitivity of 88.2% and a specificity of 64.5% in the low educational level patients, and 16/17 with a sensitivity of 77.8% and a specificity of 87.9% in the high educational level patients. Compared to the criterion MMSE≤27 and MMSE norms for the Chinese community elderly, stratified T-MoCA threshold improves correct classification by 23.7% (p=0.033) and 30.3% (p=0.020) respectively. Conclusion: T-MoCA is a feasible and effective method for MCI screening in patients with AF.
Analysis of risk factors for revision in distal femoral fractures treated with lateral locking plate: a retrospective study in Chinese patients
Authors: Hou Guojin , Zhou Fang , Tian Yun , Ji Hongquan , Zhang Zhishan , Guo Yan , Lv Yang , Yang Zhongwei , Zhang Yawen 
Published: March 2020

Abstract
Background: To analyze the risk factors of revision operation after the treatment of distal femoral fracture with lateral locking plate (LLP). Methods: Retrospective analysis of the clinical data of 152 cases with distal femoral fracture treated in our hospital from March 2005 to March 2019. SPSS 26.0 software (univariate analysis and Logistic regression analysis) was used to analyze the general condition, fracture related factors, operation related factors and construct characteristics of internal fixation. Results: 16 of 152 patients who were included in the study underwent revision surgery, with a revision rate 10.5%. Univariate analysis showed that there were significant differences in age, body mass index(BMI), fracture type, supracondylar involved or not, type of incision, quality of reduction, ratio of length of plate/fracture area(R1), the ratio of the length of the plate/fracture area above the condylar(R2), ratio of distance between proximal part of fracture and screw/working length of proximal plate(R3) between the two groups (P＜0.05). Logistic regression analysis showed that age[OR for age >61.5 group is 4.900(1.071-22.414)], fracture type[OR for A3 fracture is 8.572(1.606-45.750), the OR for periprosthetic fracture after TKA is 9.073(1.220-67.506)], poor reduction quality[OR is 7.663(1.821-32.253)] and the ratio of the length of the plate/fracture area above the condylar were the possible risk factors (P＜0.05). Conclusion: Age, fracture type (A3 and periprosthetic fracture after TKA), poor reduction quality and the ratio of the length of the plate/fracture area above the condylar were the possible risk factors of the revision in distal femoral fractures treated with lateral locking plate. The appropriate application of the locking plate and operation strategy are the key to reduce the revision rate in distal femoral fractures.
The target matters – general vs. individualized exercise prescription for fertile-aged women with metabolic risk: a randomized controlled trial
Authors: Poyhonen-Alho Maritta K , Hyrylä Vesa , Tarvainen Mika P. , Tikkanen Heikki O. , Koivusalo Saila , Koponen Anne S. , Peltonen Juha E. , Tiitinen Aila 
Published: March 2020

Abstract
Background Little is known about the associations of different depths of individualization of the exercise intervention on cardiorespiratory fitness (CRF), metabolic outcomes and cardiac autonomic regulation in at-risk subjects. This randomized trial compared the effect of general physical activity (PA) guideline -targeted and highly individualized exercise intervention on maximal oxygen uptake, heart rate variability, body composition, and glucose and lipid profiles in fertile-aged women with increased risk for gestational diabetes. Methods Forty-five women with previous gestational diabetes or BMI>30kg/m 2 were randomized into general advice without intervention (Group 1), individualized intervention planned according to PA questionnaires and general guidelines for exercise training (Group 2) and highly individualized intervention based on results from the pre-intervention cardiopulmonary exercise test (CPET) groups (Group 3). All subjects performed pre-intervention CPET on a cycle ergometer with step incremental protocol until volitional fatigue, followed by a 3-month intervention period and post-intervention CPET. Examinations included HRV assessment during CPET and body composition (bioimpedance), blood glucose and lipid profiles. Results Total dropout was 53.8% at various points of the study leaving 8 subjects in Group 1, 12 subjects in Group 2 and 10 subjects in Group 3. CRF improved only in Group 3 (+1.9 ml/kg/min, 95% CI 0.3 to 3.5). This was associated with an increase in high-density lipoprotein (0.18 mmol/L, 95% CI 0.04 to 0.32) and increased HRV. In Group 2, we found a decrease in body mass index (-0.7 kg/m 2, 95% CI -1.3 to -0.1), fasting insulin (-4.14 mU/L, 95% CI -6.58 to -1.70), insulin resistance (-1.21, 95% CI -1.88 to -0.54), and low-density lipoprotein (-0.44 mmol/L, 95% CI -0.79 to -0.09). The dropouts in Group 1 had significantly less weight, smaller waist circumference, less visceral fat, and higher maximal oxygen uptake compared to the continuers in Group 1. Conclusions To improve CRF and cardiac autonomic function the exercise intervention should be highly individualized. PA intervention focused to achieve general exercise guidelines is not enough to improve CRF over 3-month period but combined with weight loss has beneficial effects on the metabolic profile. In randomized controlled trials, dropout may be biased. Trial Registration clinicaltrials.gov (NCT01675271)
Identification and characterization of metastasis-associated individualized gene expression signature in osteosarcoma
Authors: Zhang Guofeng , Zhu Yonglin , Jin Chengzhen , Zhou Baisui , Zhao Shanrun , Li Chuanbo , Fu Fangang , Li Shanhui 
Published: March 2020

Abstract
Introduction: Osteosarcoma (OS) patients with complete surgical resection still relapse with poor prognosis. Part of this is due to the inability to accurately detect distant metastasis. Thus it’s enssential to identify metastasis-related biomarkers for OS.Methods: In present study, a computational pipeline based on relative expression orderings (REOs) using gene expression proles was constructed in metastases and non-metastases OS patients. Results: 138 metastasis-associated gene pair signature (MGPS) were identified follow two independent datasets. In order to further extract metastasis-associated biomarker for clinical application, a metastases-specific co-expressed MGPS network was constructed and analyzed. MGPS such as MYL5 and RPL27A showed strong positive correlation (Cor =0.75, P <0.001) in metastatic OS patients. There were thirteen MGPSs in above network were associated with prognosis. These prognostic MGPSs could become as a specific classifier to distinguish metastases and non-metastases OS patients. Functional analysis showed MGPSs were associated with cancer metastasis-related functions. Drug and MGPS network could provide some drug candidates for treatment of OS. Conclusions: Collectively, the roles of the MGPSs in OS were elucidated, which could be beneficial for understanding OS pathogenesis and treatment.
Chronic lymphocytic thyroiditis (CLT) has a negative prognostic value in papillary thyroid cancer of the young and middle ages: a retrospective analysis of 635 patients
Authors: Ma Bing'e , Chen Xiyi , Zhao Zhengping , Ji Qin , Zhou Yifan , Yin Xiaoyang , Ma Chaoqun , Wang Jianhua 
Published: March 2020

Abstract
Background: The study was aimed at investigating the potential role of chronic lymphocytic thyroiditis (CLT) in papillary thyroid cancer (PTC) prognosis in distinct age groups, as well as the association between CLT and recurrence risk estimation.Methods: A total of 635 adult patients were retrospectively analyzed. On the basis of postoperative pathology examination, 188 patients were diagnosed with coexistent CLT and the remaining 447 were classified as non-CLT. Then the characteristics of CLT-coexisted patients and non-CLT ones were compared respectively when patients were aged ≥55 years or below. The association among postoperative clinicopathological features were also analyzed using multivariate regression. In addition, the prognostic value of several variables relating to high-risk recurrence were estimated within different age groups.Results: When divided in two age groups (55 years as the line), non-CLT group had a remarkable frequency of small size lesion (D max ≤1cm) compared with CLT-coexisted patients (54.6% to 43.0%, p =0.016). In addition, non-CLT patients tended to have intrathyroidal extension as opposed to those with coexistent CLT (20.2% to 28.2%, p =0.05). In multivariate analysis, CLT still significantly acted as an independent risk factor of greater lesion size (D min >1 cm) (OR=1.7, p =0.02) and mildly promoted gross extrathyroidal extension (ETE) (OR=1.4, p =0.06). However, associations didn't emerge in the characteristics mentioned above with CLT when patients were ≥55 years old. The prognostic value of CLT in high-risk recurrence was evident only in patients aged 35-44 years. (OR=2.4, 95%CI:1.2-5.4, p =0.02). Greater lesion size independently promoted gross ETE, no matter patients were aged above 55 years or not. Besides, its prognostic value of high-risk recurrence was significant throughout all age groups. Conclusion: These findings revealed that CLT coexistence might be the unfavorable factor of PTC prognosis in young and middle-aged (<55 years) patients, and its role in recurrence risk stratification was presented only in the specific age (35-44 years).