bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of Date Fruit (Phoenix Dactylifera L.) on Labor and Delivery Outcomes: A Systematic Review and Meta-Analysis
Authors: Karimi Alireza Bagherzadeh , Elmi Asghar , Mirghafourvand Mojgan , Navid Roghyeh Baghervand 
Published: February 2020

Abstract
Background: The rate of cesarean section is increasing in all over the world with different drafts in various countries. This growth increases unpleasant outcomes of delivery. Recent studies explained the benefits of date palm fruit on labor process improvement. Date fruit can be considered as a factor for increasing vaginal delivery and also reducing the frequency of caesarean section in order to prevent its great complications. This systematic review has been designed to review clinical studies that investigate the effects of date palm fruit on labor outcomes (duration of labor stages, bishop score, and frequency of cesarean section) compared with routine cares. Methods: This study was performed in 2019. Required data has been collected from electronic databases and manual searches. All randomized clinical trials evaluating the effects of date palm fruit on labor and delivery that were published from January 2000 to August 2019 in English and Persian languages, were incorporated in this systematic review. The methodological quality of the included studies was evaluated according to the risk of bias assessment of Cochrane handbook of systematic reviews, and were then reported using Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) statement. Results: Eight studies were included in the qualitative and quantitative synthesis. Meta-Analysis showed that date fruit consumption can significantly reduce active phase of labor (three trials with 380 participants; (MD= -109.3, 95%CI (-196.32, -22.29; I2=89%), P=0.01), and also it can significantly improve the bishop score (two trials with 320 participants; MD = 2.45, 95%CI (1.87, 3.04; I2=0%), P<0.00001). Date fruit consumption had no effects on the duration of first, second, and third stages of labor, and the frequency of cesarean section. Conclusion: Date can reduce the duration of active phase and improve the bishop score; however, due to from the low to mediate quality of the studies; it seems that the other studies are needed to prove these results better than this.
Comparative genomic analysis of 142 bacteriophages infecting Salmonella enterica subsp. enterica
Authors: Gao Ruimin , Naushad Sohail , Moineau Sylvain , Levesque Roger , Goodridge Lawrence , Ogunremi Dele 
Published: February 2020

Abstract
Background: Bacteriophages are bacterial parasites and are considered the most abundant and diverse biological entities on the planet. Previously we identified 154 prophages from 151 serovars of Salmonella enterica subsp. enterica . A detailed analysis of Salmonella prophage genomics is required given the influence of phages on their bacterial hosts and should provide a broader understanding of Salmonella biology and virulence and contribute to the practical applications of phages as vectors and antibacterial agents. Results: Comparative analysis of the full genome sequences of 142 prophages of Salmonella enterica subsp. enterica retrieved from public databases revealed an extensive variation in genome sizes (6.4- 358.7 kb) and guanine plus cytosine (GC) content (35.5-65.4%) and a linear correlation between the genome size and the number of open reading frames (ORFs). We used three approaches to compare the phage genomes. The NUCmer/MUMmer genome alignment tool was used to evaluate linkages and correlations based on nucleotide identity between genomes. Multiple sequence alignment was performed to calculate genome average nucleotide identity using the Kalgin program. Finally, genome synteny was explored using dot plot analysis. We found that 90 phage genome sequences grouped into 17 distinct clusters while the remaining 52 genomes showed no close relationships with the other phage genomes and are identified as singletons. We generated genome maps using nucleotide and amino acid sequences which allowed protein-coding genes to be sorted into phamilies (phams) using the Phamerator software. Out of 5796 total assigned phamilies, one phamily was observed to be dominant and was found in 49 prophages, or 34.5% of the 142 phages in our collection. A majority of the phamilies, 4330 out of 5796 (74.7%), occurred in just one prophage underscoring the high degree of diversity among Salmonella bacteriophages. Conclusions: Based on nucleotide and amino acid sequences, a high diversity was found among Salmonella bacteriophages which validate the use of prophage sequence analysis as a highly discriminatory subtyping tool for Salmonella. Thorough understanding of the conservation and variation of prophage genomic characteristics will facilitate their rational design and use as tools for bacterial strain construction, vector development and as anti-bacterial agents.
Comparison between flipped classroom and team-based learning in a prosthodontic class at Tokushima University
Authors: Hayama Rika , Okura Kazuo , Oshima Masamitsu , Hosoki Maki , Suzuki Yoshitaka , Miyagi Mayu , Inoue Miho , Rodis Omar Marianito Maningo , Matsuka Yoshizo 
Published: February 2020

Abstract
Background: Active learning is a concept that allows students to study and learn actively by themselves to get knowledge. There are several methods of active learning, including flipped classroom (FC) and team-based learning (TBL). In FC, students are required to study before classes. In TBL, students study before the class, take the Individual Readiness Assurance Test (IRAT) and Group Readiness Assurance Test (GRAT), and then discuss Group Assignment Projects (GAPs) during class. The purpose of this study was to compare the effectiveness between FC and TBL using longitudinal term-end examination data. Methods: Flipped classroom and TBL effectiveness was assessed from the results of the term-end examinations at the end of the semester from 2014 to 2017. The students were asked to answer questions on the favorable and unfavorable responses of FC and TBL. To check the difficulty of the term-end examinations, control dentists took the same examinations. The dentists were clinical prosthodontic residents who graduated within 3 years from undergraduate course. All prosthodontic residents attended the referential examinations. Results: The term-end examination score of FC and TBL did not show a statistical difference. Multi-way ANOVA showed that the referential examination scores by the dentists were significantly higher than that of the students (P < 0.0001). According to the students, the favorable responses of FC and TBL were on the study habit and the video contents, while the unfavorable responses were mainly on the study materials. Conclusions: There is no statistical difference between FC and TBL on term-end examination scores. There were no interactions between the test period and the participants (students or dentists), and the test period and class format.
Comparative Study of the Clinical Competence of Emergency Nurses Using Self-Assessments and Head Nurse Evaluations
Authors: ghahfarokhi motahareh musavi , Mehrafruz Elina , Radmehr Arman , kiarsi maryam , Shamloo Marziyeh Beigom Bigdeli 
Published: February 2020

Abstract
Background: Clinical competency is the ability of nurses to play a professional role in a clinical environment, especially in an emergency department, in terms of the quality of the services provided. The present study aimed to compare the clinical competence of emergency department nurses using self-assessments and evaluations by head nurses. Method: A descriptive-analytical study was conducted from July to September 2017. The census method was used to select 70 nurses working in the emergency departments of three hospitals. Data was collected by using self-assessment questionnaires and evaluations by head nurses. The instrument was a clinical competency questionnaire with questions related to seven functional fields and 63 skills. The data obtained from descriptive and inferential statistics were analyzed by SPSS-16. Result: The viewpoints of nurses and head nurses on the clinical competencies of nurses were assessed as being at a moderate level in the majority of domains. Nurses defined their clinical competencies at a significantly higher level than the head nurses (P<0.05). Conclusion: The periodic assessment of emergency nurses, as a critical part of the hospital, can guide nursing managers to pay attention to professional competence and promote continuing education programs for improving their competencies.
Lysyl oxidase G473A (rs1800449) polymorphism influences the risk of cancer progression: a meta-analysis
Authors: Mongkolrob Rungrawee , Tharabenjasin Phuntila , Bualuang Aporn , Pabalan Noel 
Published: February 2020

Abstract
The genetics of cancer progression is important for the design of optimal therapeutic strategies. Lysyl oxidase (LOX) is a cancer progression gene that has been studied enough to warrant a synthesis of its primary data from association studies. However, reported associations between the LOX G473A (rs1800449) gene polymorphism and cancer have been inconsistent, prompting a meta-analysis so that we could obtain more precise estimates. Database searches of the published literature yielded seven case-control studies. We calculated pooled odds ratios (ORs) and 95% confidence intervals (CIs) using four genetic models: homozygous (H), recessive (R), dominant (D) and codominant (C). Subgroup analysis was based on ethnicity and cancer type. Outlier analysis was used to examine sources of heterogeneity. The strength of evidence was based on the magnitude of effects, high associative significance, consistency and homogeneity. H/R outcomes exerted more associative effects than D/C results. Two reasons for this are as follows: (i) H/R analyses precluded outlier treatment; and (ii) the magnitude of H/R (ORs of > 2.0) was twice that of D/C (ORs > 1.0). All else being equal, significant pooled ORs in all genetic models had high significance (Pa < 10-5). Strong evidence for associations was found in the outcomes for Asian and gastrointestinal (GI) cancers. In summary, the LOX rs1800449 polymorphism confers significant overall susceptibility, particularly in GI cancers, and places Asians at risk.
Tumor microenvironment characterization and Immune infiltration in head and neck squamous cell carcinoma
Authors: Wu Zenghong , Niu Xun , Xiao Xi-Yue , Chen Xiong 
Published: February 2020

Abstract
Background : Head and neck squamous cell carcinoma (HNSCC), a basic malignant tumor of the head and neck distinct. As a famous heterogeneous disease, the jobs of immune cells inside the tumor-related as yet missing for HNSCC, particularly in current immunotherapy. Method: We explored the TME, TMB and evaluate the 22 TIICs subsets of immune response based on GEO and TCGA database of HNSCC to explore its relationship with atomic subpopulation, survival, function and expression difference and reveal potential targets and biomarkers for immunotherapy. Results: Observing the download of GSE6631 database contained 22 HNSCC samples and 22 normal samples and TCGA database contained 111 HNSCC and 12 normal tissues. The results suggested that the expression of macrophages M0 and T cells CD4 memory resting was significant difference and may plays an important role in regulate cancer progression ( P <0.05). The result of tumor mutational burden revealed that the most common somatic mutations variant classification was missense mutation, the most common DNA sequence polymorphism type was SNP, the most common single nucleotide variants (SNV) class was C>T, the variants per sample median was 78 in HNSCC patients. Top 10 mutated genes that related to TMB was TP53, TTN, FAT1, MUC16, CDKN2A, CSMD3, SYNE1, LRP1B, NOTCH1 and PIK3CA. We portrayed the immune scene in detail, uncovering the awesome immune infiltration styles of various subtypes in HNSCC. Conclusion: The intricate connection between TIIC, TMB and genomic alterations was additionally set up. Our paintings advance the information of immune response and offers significant assets for research to enhance immunotherapy.
Comparison of the Therapeutic Outcomes between Open Plantar Fascia Release and Percutaneous Radiofrequency Ablation in the Treatment of Intractable Plantar Fasciitis
Authors: Yuan Yusong , Qian Yuan , Lu Hao , Kou Yuhui , Xu Yangbo , Xu Hailin 
Published: February 2020

Abstract
Background: Heel pain is one of the most common complaints in foot and ankle clinic and one of the leading causes of heel pain is plantar fasciitis. Methods: A retrospective analysis was carried out in 31 cases (39 feet) of patients with intractable plantar fasciitis. In the enrolled 26 cases, there were 16 patients (19 feet) received open plantar fascia release, and the other 15 patients (20 feet) received percutaneous radiofrequency ablation. The surgical results were assessed by visual analogue scale (VAS) and American orthopaedic foot and ankle society ankle-hindfoot scale (AOFAS-AH) before and after surgery in all patients. Results: All 31 patients were followed up successfully, with a mean follow-up time of 58.77 months. There were no differences of patient’s demographics and characteristics information between the two groups. The average operative time of the feet in the open plantar fascia release is longer time than the percutaneous radiofrequency ablation. Furthermore, the percutaneous radiofrequency ablation group had a shorter recovery time to normal activity than the open plantar fascia release group. There were no difference of postoperative VAS scores and the AOFAS-AH scores between the two groups. All patients reported satisfaction after either operation. Conclusion: Symptoms of pain and limb function were significantly improved in patients both of the partial plantar fascia release treated group and the percutaneous radiofrequency ablation treated group. The two types of surgical procedures shared the same long-term curative effects. However, percutaneous radiofrequency ablation was a better technique from the point of shorter operative time and postoperative recovery time.Trial registration: Retrospectively registered.
Production and Concentration of Recombinant Lentiviral Particles
Authors: McCullough Shaun D. 
Published: February 2020

Abstract
This protocol describes the production of recombinant lentivirus by transfection of HEK293T cells with lentiviral packaging vectors and subsequent concentration by ultracentrifugation. The attached methods document is a formal version of the information included here.Disclaimer: The contents of this article have been reviewed by the US Environmental Protection Agency and approved for publication and do not necessarily represent Agency policy. Mention of trade names or commercial products does not constitute endorsement or recommendations for use.
Fixation and staining of Drosophila L1 larval brains for immunofluorescence microscopy and preparation for live imaging
Authors: Ly Phuong Thao , Thompson John D. , Endow Sharyn A. 
Published: February 2020

Abstract
Drosophila first instar (L1) larval brains (LBs) contain frequent quiescent neural stem cells (qNSCs) as well as activated neuroblasts, making them favorable for studying stem cell quiescence and activation. However, the small size of LBs at the L1 stage necessitates the use of modified methods to prepare the LBs for immunofluorescence microscopy (IFM). The protocol described here allows efficient collection of embryos and maturation of larvae to the mid-L1 stage, followed by dissection, fixation and processing of LBs through the antibody staining steps for IFM. The entire procedure can be completed in ~3-5 days. Methods are also described for use in preparing L1 brains for live imaging experiments, including a file to create accessible and cost-effective 3D printed slides that can be fit with an O2-permeable membrane for live imaging.
Single Level ACCF Combining Single Level Stand-alone ACDF in Treating Cervical Spondylotic Myelopathy
Authors: Zhang Zhichao , Chen Jingxuan , Shi Wei , Zhou Shengyuan , Chen Xiongsheng , Jia Lianshun 
Published: February 2020

Abstract
Objective Single level ACCF combining single level ACDF (AcA) is an ideal way to treat CSM with multiple consecutive cervical intervertebral disc herniation alongside with severe bony narrowing of the spinal canal in between. AcA with the stand-alone technics on the ACDF level and a shorter titanium plate that only covers the ACCF levels (AcAsa) may possess potential advantages than conventional AcA. We performed a retrospective study to evaluate the feasibility, safety and effectiveness of AcAsa procedure. Methods 379 patients with CSM who had conventional AcA or AcAsa were retrospectively reviewed. Related patients factors, disease factors and treatment factors were acquired and subjected into student’s t test, chi-square test and survive analyses. Results Preoperative and postoperative JOA Scores in AcAsa: p<0.001. Preoperative and postoperative VAS Scores in AcAsa: p<0.001. 12months improvement of JOA Score between preoperative VAS Score≥2 and <2 in AcAsa: p=0.002. The amount of intraoperative blood loss between AcAsa and conventional AcA: p=0.011. Incidence of postoperative dysphagia between AcAsa and conventional AcA: p=0.038. Conclusion AcAsa significantly improve patients’ JOA Scores and reduce VAS Scores. Compared with conventional AcA, AcAsa leads to smaller amount of intraoperative blood loss and lower incidence of postoperative dysphagia. The alleviations of neurological symptoms, cervical motions and disc space heights at the ACDF levels are similar between the two types of surgeries. And patients with preoperative VAS less than 2 may benefit more from an AcAsa procedure.
Study on the job performance of Chinese physicians: a moderated mediation model
Authors: Guo Botang , Qiang Binbin , Chu Haiyun , Zhou Jiawei , Yang Xiuxian , Qiu Xiaohui , Qiao Zhengxue , Song Xuejia , Zhao Erying , Yang Yanjie , Cao Depin 
Published: February 2020

Abstract
Background:To explore the mechanisms between achievement motivation and job performance among physicians of public hospitals in China, this article researched the influence of different personality traits on physicians' job performance. Methods: A survey conducted in 2017, containing samples of 1,523 physicians from eight tertiary grade A hospitals in Harbin, China.A cross-sectional study was applied to data collection about physicians’ achievement motivation, job performance, organizational commitment, personality traits and other demographic variables. This paper also analyzed the relationships between physicians’ job performance and achievement motivation using a moderated mediation model.Independent t-test and ANOVA were applied to describe and compare the demographic data. Pearson correlation coefficients were used to examine the correlation among the variables. To test the correlation of the eight variables, moderated mediation analysis was performed in this study. Results:Achievement motivation directly affects job performance of Chinese physicians, and organizational commitment partially mediates the direct effects of achievement motivation on job performance. Additionally, we also found that agreeableness and conscientiousness moderate the strength of the relationships between achievement motivation and job performance mediated by organizational commitment. Conclusions:Managers in Chinese public hospitals should pay attention to increase physicians’ level of achievement motivation, and we recommend to enhance organizational commitment through a reasonable compensation system and active organizational culture. And they should also tend to be conscientiousness or agreeableness in the arrangement. Key Words job performance; hospital management; achievement motivation; psychology; moderated mediation model
How nursing students’ placement preferences and perceptions of community care develop in a more ‘community-oriented’ curriculum; A longitudinal cohort study
Authors: Iersel Margriet van , Latour Corine , Rijn Marjon van , de Vos Rien , Kirschner Paul A. , Reimer Wilma Scholte op 
Published: February 2020

Abstract
Background: Extramuralisation in healthcare has influenced medical and nursing curricula internationally with the incorporation of themes related to primary/ community care. Despite this, students do not easily change their career preferences. The hospital is still favourite, leading to labour market shortages in extramural care. This study investigates how baccalaureate nursing students’ perceptions of community care and placement preferences develop over time in a more 'community-care-oriented' curriculum, to gain insights on which curriculum elements potentially influence career choices.
Methods: A nursing student cohort of a University of Applied Sciences in the Netherlands ( n = 273) underwent a new four-year curriculum containing extended elements of community care. The primary outcome was assessed with the Scale on Community Care Perceptions (SCOPE). Data were collected each year of study. Descriptive statistics were used to investigate students’ placement preferences and perceptions, and linear mixed model techniques (LMMs) for measuring how students’ perceptions develop over time. Patterns of placement preferences at individual level were visualised.
Results: Students’ perceptions of community care, as measured with SCOPE, show a slight decrease between year 1 and 4, while items mutually differ substantially. In contrast, the preference of community care for a placement increases from 2.6% in year 1 tot 8.2% in year 4. The hospital is favourite in year 1 (79.8%), and remains most popular. At individual level, students often change placement preferences, although a preference for the hospital is more consistent. The LMMs indicates that, at the four time-points, the estimated marginal means of students’ perceptions fluctuate between 6 and 7 (range 1-10). A placement in community care did not positively influence students’ perceptions, and an intensive one week theoretical programme was only temporarily influential.
Conclusion: Although interest for placement in community care increased substantially, it was not clear which curriculum elements stimulated this, nor did the curriculum positively influence students’ perceptions. As most students do not look forward to the high responsibility of the field, other curricula with educational tracks for more mature students/ nurses with a vocational training may be an alternative contribution to solving the labour market problems in community care.