bluuebunny/crossref_metadata_2025_split huggingface dataset:
APEX-Gold: A genetically-encoded particulate marker for robust 3D electron microscopy
Authors: Rae James , Ferguson Charles , Ariotti Nicholas , Webb Richard I. , Cheng Han-Hao , Mead James L. , Riches Jamie , Hunter Dominic J.B. , Martel Nick , Baltos Joanne , Christopoulos Arthur , Bryce Nicole S. , Cagigas Maria Lastra , Fonseka Sachini , Hardeman Edna C. , Gunning Peter W. , Gambin Yann , Hall Thomas , Parton Robert G. 
Published: October 2020

AbstractGenetic tags allow rapid localization of tagged proteins in cells and tissues. APEX, an ascorbate peroxidase, has proven to be one of the most versatile and robust genetic tags for ultrastructural localization by electron microscopy. Here we describe a simple method, APEX-Gold, which converts the diffuse oxidized diaminobenzidine reaction product of APEX into a silver/gold particle akin to that used for immunogold labelling. The method increases the signal to noise ratio for EM detection, providing unambiguous detection of the tagged protein, and creates a readily quantifiable particulate signal. We demonstrate the wide applicability of this method for detection of membrane proteins, cytoplasmic proteins and cytoskeletal proteins. The method can be combined with different electron microscopic techniques including fast freezing and freeze substitution, focussed ion beam scanning electron microscopy, and electron tomography. The method allows detection of endogenously expressed proteins in genome-edited cells. We make use of a cell-free expression system to generate membrane particles with a defined quantum of an APEX-fusion protein. These particles can be added to cells to provide an internal standard for estimating absolute density of expressed APEX-fusion proteins.
Determinants of the annual rate of change of union membership in divergent national contexts after the nations survive through major economic crisis
Authors: Sarkar Santanu , Chakraborty Ranabir 
Published: October 2020

PurposeWe were intrigued by the question of whether the convergence of businesses across nations in search of flexibility to survive economic crisis led to a convergence of the annual rate of change of union membership. The question emerged because the convergence theory was controverted, especially when the neo-capitalist idea failed to withstand the test of time during the economic crisis.Design/methodology/approachBy adopting the model from Bain and Elsheik (1976) and using time-series data from 1990 to 2014 for Finland and India that survived economic crisis during this period, whereby union membership remained steady in Finland but declined in India, we assessed the empirical distinction between the changes in union membership.FindingsWe argued that when hit by an economic crisis, different nations had divergent responses and chose different means of economic recovery because of which the countries have not withstood the crisis in one specific way/direction that at all times, marginalises unions. Our main finding is that in both the countries, the annual rate of change of union membership during the years of economic recovery was determined by the policy response. And, policy responses were determined not only by the causes of economic crisis but also by the strength of unique national institutional configurations and history of the country.Originality/valueThe annual rate of change of union membership during the years of economic recovery was determined by the policy response. And, policy responses were determined not only by the causes of economic crisis but also by the strength of unique national institutional configurations and history of the country.
Goals of the national mathematics curriculum of Pakistan: educators’ perceptions and challenges toward achievement
Authors: Mughal Shahid Hussain , Asad Muhammad Mujtaba , Adams Donnie 
Published: October 2020

PurposeThe national mathematics curriculum of Pakistan has emphasized on improving content knowledge, reasoning abilities and problem-solving skills of students about thinking, communicating and solving mathematics (national mathematics curriculum of Pakistan, 2006). Whereas, there is a need to understand the point of view of teachers about the challenges they face in achieving the goals of national mathematics curriculum. This will help leading teacher training institutions to revisit their math teacher continuous professional development (CPD) programs and facilitate school leadership in improving the quality of math education in rural schools of the province. However, the purpose of this research study is to figure out the challenges that teachers are facing while achieving the goals of the national curriculum by teaching mathematics at the primary level in educational institutes of Pakistan.Design/methodology/approachIn this research study qualitative research approaches have been utilized, in which focus group discussions (FGDs) were used as data collection techniques. Furthermore, thematic analysis of the data led toward the development of four overarching themes such as teachers' knowledge about mathematics curriculum, challenges relating to mathematics content and pedagogy, difficulties in developing conceptual understanding and designing lesson plans to address students' diversity.FindingsThe overall findings of this research study suggested that the majority of teachers are facing difficulties in mathematics content teaching such as decimal fraction, unitary method, measurement principles, practical geometry and data handling. Moreover, teachers are also facing challenges and difficulties in developing hands-on and minds-on activities in the teaching of mathematical concepts to the students of primary level in educational institutes of Pakistan.Practical implicationsThis research study will facilitate the teachers and stakeholders to address the problematic issues in the domain of content delivery of mathematics. Whereas, this study recommends educating teachers about national mathematics curriculum and to develop a CPD framework for mathematics teachers for the enhancement of their pedagogical content knowledge. The study also recommends orientating school heads about the different aspects of math curriculum so that they can mentor math teachers in achieving math curriculum goals.Originality/valueThis is the first research study of its nature, which targets and highlights the teacher's perceptions toward the achieving the goals of national mathematics curriculum of Pakistan and addressing the pedagogical challenges faced in mathematics teachers. There is a dearth of studies in mathematics education in Sindh province. The issue is of immense importance, the findings will help teachers to improve mathematics instructions at primary level.
Critical variables and constructs in the context of project management: importance-performance analysis
Authors: Haverila Matti , Haverila Kai Christian , Twyford Jenny Carita 
Published: October 2020

PurposeRelying on the importance-performance theory first established by Martilla and James (1977), this research paper utilizes a unique statistical analysis instrument embedded into the SmartPLS software. It explores the importance and performance of key project management constructs and indicators with a purpose to make practical and actionable recommendations for project managers to identify and improve project management practices.Design/methodology/approachThe data used were derived from 3,130 system delivery projects in the facilities management industry. The data was analyzed with Partial Least Squares Modelling (PLS) software SmartPLS, using its embedded importance-performance functionality.FindingsThe findings indicate the importance and performance of the project management constructs and their respective indicator variables in an importance-performance (IPMA) map. All three project management phases (constructs); proposal, installation and commissioning, were significantly related to satisfaction. The installation phase (construct) showed the highest potential for performance improvement in project management. With regard to the specific indicator variables, the variable “Coordinating their work with other contractors (or the owner's staff)” received a strong “Do better” recommendation.Originality/valueThe approach and results provide an easy to use and visual tool for project managers to assess the importance and performance of the various elements of project management. The instrument provides a project management direction for the identification of strategic enhancement areas as it is essential to recognize what facets of project management contribute most to the improvement of project management performance over a longer period of time (Cronin and Taylor, 1992; Palmer, 1998).
Linking work, occupational identity and burnout: the case of managers
Authors: Hamouche Salima , Marchand Alain 
Published: October 2020

PurposeBased on identity theory, identity represents a set of meanings individuals hold for themselves based on their role in the society. Hence, they often engage in the process of verifying their role, seeking for the compatibility between these meanings and those perceived in a specific lived situation. If this compatibility is not perceived, this is likely to generate negative emotions. that could compromise their mental health. This paper examines the contribution of a weak verification of role identity in the explanation of managers ‘burnout. It aims at integrating identity theory into occupational stress research by analysing the proposition that a low level of verification of a salient role-identity will be associated with a high level of burnout. Hence, we consider identity salience as a moderating variable.Design/methodology/approachCross-sectional data of 314 Canadian managers employed in 56 Quebec firms. Multilevel regression analyses were performed to analyse the data.FindingsLow levels of verification of some standards of managers' role identity, mainly work demands and recognition which encompasses (monetary and non-monetary recognition, career prospects and job security) are significantly associated with managers' burnout. Furthermore, as predicted, results show that identity salience plays a moderating role on the relation between a weak verification of some standards of managers' role identity and burnout, mainly work demands, superior support and recognition.Originality/valueThis study proposes a relatively unexplored approach for the study of managers' burnout. It broadens the scope of research on workplace mental health issues, by the integration of the identity theory.
Nationalistic appeals and consumer boycotts in China, 1900-1949
Authors: Yan Tony , Hyman Michael R. 
Published: October 2020

Purpose
The purpose of this study is to explore how nationalistic appeals may affect consumers’ perception and purchasing of targeted brands. Qualitative historical data from old China (1900–1949) reveal that social movement groups can adopt nationalistic appeals assisted by meaning framing – defined as a creative interpretation of symbols, designs, behaviors, social events and cultural identities to serve social and political goals – to shape consumers’ attitudes toward foreign brands. After examining the mechanisms and processes underlying consumer boycotts from 1900 to 1949, the responsive strategies of affected foreign companies are illustrated.

Design/methodology/approach
Critical historical research method is applied to historical data and historical “traces” from China’s corporate documents, memoirs, posters, advertisements, newspapers and secondhand sources documenting Chinese boycotts from 1900 to 1949.

Findings
Consumers may pursue interests beyond economic interests. Nationalistic appeals can mobilize consumer boycotts against foreign brands that were perceived to support or relate to targeted countries. Political framing of certain events shapes consumers’ perceptions and concomitant brand choices.

Research limitations/implications
Although differences between historical and current contexts may require tailoring past marketing strategies to current conditions, past strategies can inform current and future strategies.

Practical implications
Strategies adopted by foreign companies in old China (1900–1949) can help contemporary companies design effective marketing strategies for a hostile marketplace infused with nationalistic appeals and competing interests.

Social implications
Although local companies can adopt economic or political nationalism to realize their economic goals, it represents a double-edged sword that can harm national brands.

Originality/value
A historical analysis of nationalistic business appeals in pre-1949 China can inform the counterstrategies modern companies adopt to overcome consumer boycotts.
Identity leadership and well-being: team identification and trust as underlying mechanisms
Authors: Krug Henning , Geibel Hannah V. , Otto Kathleen 
Published: October 2020

PurposeThe purpose of the present research was to examine the impact of identity leadership on employees' well-being mediated by team identification and trust in the leader.Design/methodology/approachIn study 1, N = 192 employees participated in a cross-sectional online survey measuring identity leadership, team identification, trust in the leader and well-being (i.e., job satisfaction, work engagement, burnout). In study 2, N = 72 university students participated in a vignette study that manipulated high/low identity leadership and tested its effect on team identification and trust in the leader.FindingsIn study 1, identity leadership predicted higher team identification, trust in the leader and well-being of employees. Team identification mediated the positive relationship of identity leadership with both job satisfaction and work engagement, while trust in the leader mediated the negative relationship of identity leadership with burnout. In study 2, team identification and trust in the leader were significantly higher in the high identity leadership condition.Research limitations/implicationsThe findings are consistent with the few existing studies on the positive effects of identity leadership. However, due to the correlational nature of the data in study 1, future longitudinal field research is needed to support the current findings and further establish causality for the model as a whole.Practical implicationsIdentity leadership seems to be promising to increase well-being among employees. Thus, leadership development programs to foster identity leadership and collective identity should be implemented in organizations and further tested with respect to well-being.Originality/valueThis research contributes to an emerging body of research on the social identity approach to leadership and supports the recent claims that social identity might be one of the links between leader behavior and well-being of employees. Moreover, this study is among the first to investigate and experimentally test the underlying mechanisms of identity leadership.
The thermomechanical response of a poroelastic medium with two thermal relaxation times
Authors: Abbas Ibrahim , Hobiny Aatef 
Published: October 2020

PurposeThe purpose of this paper is to study the wave propagation in a porous medium through the porothermoelastic process using the finite element method (FEM).Design/methodology/approachOne-dimensional (1D) application for a poroelastic half-space is considered. Due to the complex governing equation, the finite element approach has been adopted to solve these problems.FindingsThe effect of porosity and thermal relaxation times in a porothermoelastic material was investigated.Originality/valueThe numerical results for stresses, displacements and temperatures for the solid and the fluid are represented graphically. This work will enable future investigators to have the insight of nonsimple porothermoelasticity with different phases in detail.
Personal and organizational level relationships in relational exchanges in supply chains – a bottom-up model
Authors: Qian Chen , Seuring Stefan , Wagner Ralf , Dion Paul A. 
Published: October 2020

Purpose
This paper aims to examine how trust and communication at the personal level relationships conform to trust and communication at the organizational level relationships and which role do the two different level relationships play in influencing firms’ commitment, performance and propensity to stay in long-term relationships.

Design/methodology/approach
A face-to-face questionnaire study was conducted using a sample of 209 in Mainland China companies, which were surveyed in nine exhibitions. The data were analyzed using structural equation modeling.

Findings
The results support the bottom-up effect of interpersonal trust and communication on inter-organizational trust and communication. Interorganizational trust has a more powerful total effect on firm commitment. Interpersonal communication has a more powerful total effect on inter-organizational trust and communication and firms’ operational performance. Interpersonal communication, inter-organizational trust and communication have comparably high impacts on firms’ propensity to stay in long-term relationships.

Research limitations/implications
This paper selects Mainland China as the research context and targets a single boundary spanner in each respondent firm to evaluate both the interpersonal and inter-organizational relationships. A cross-sectional approach was used.

Practical implications
This paper suggests that business people should pay attention to the role of human factors in a firm’s relational exchanges with SC partners and effectively use the positive effects of these factors to create relationship-building benefits.

Originality/value
This paper conducts cross-level research, which has been called for in recently published inter-organizational literature. It develops and provides empirical evidence for a bottom-up model from interpersonal relationships to inter-organizational relationships and identifies their impacts on organizational outcomes simultaneously.
3D forensic facial approximation: Implementation protocol in a forensic activity
Authors: Baldasso Rosane Pérez , Moraes Cicero , Gallardo Elisa , Stumvoll Monica Bujes , Crespo Kleber Cardoso , Strapasson Raíssa Ananda Paim , de Oliveira Rogério Nogueira 
Published: October 2020

AbstractThe primary objective of this paper is to report on the successful implementation of forensic facial approximation in a real case in the forensic context. A three‐dimensional (3D) facial approximation protocol of the skull was performed with free software, applying techniques in a virtual environment that have already been consolidated in the literature. The skull was scanned with the photogrammetry technique, the digital replica was imported in the Blender software (Blender Foundation, Amsterdam) and individualized model sketches of the face were traced with the MakeHuman software (MakeHuman Org) according to the anthropological profile of the victim. The face created was imported in Blender, where it was adapted, modeled, and sculpted on the 3D skull and its soft tissue markers, using an American open‐source application of the technique in the digital environment. The face created in a virtual environment was recognized and legal identification procedures were started, resulting in the more agile delivery of the disappeared body to its next of kin. It is therefore concluded that facial approximation may not be a primary method of human identification, but it can be satisfactorily applied in the forensic field as an individual recognition resource. It has great value in narrowing the search, reducing the number of alleged victims, and leading to identification tests, therefore significantly reducing the number of genetic DNA (deoxyribonucleic acid) tests—which are considered costly for the State or Federation—and consequently reducing the waiting time before delivery of the body to its family.
Factors influencing duration of compensation following road traffic crash injury in older vs younger adults
Authors: Brown Katherine , Cameron Ian D. , Keay Lisa , Rogers Kris , Nguyen Ha , Vines Prue , Ivers Rebecca 
Published: October 2020

AbstractObjectiveTo identify factors independently associated with late claim closure, a proxy for recovery, in older (65+ years) compared to younger (17‐64 years) adults following road traffic crash injury (RTI).MethodsMultivariable analysis of statewide compensation data from New South Wales (NSW), Australia, for NSW residents aged ≥17 years injured in a non‐catastrophic RTI between July 2010 and June 2013 with finalised claims (n = 16 687).ResultsLegal representation was the dominant factor independently associated with late claim closure in both age groups. However, the odds were doubly high for the younger group (younger: AOR 8.46, 95% CI 7.7‐9.4; older: AOR 3.95, 95% CI 3.1‐5.0). Median time to claim closure was shorter for older people.ConclusionOlder (and younger) claimants with legal representation are at increased risk of late claim closure and can be readily identified within routinely collected compensation data, enabling delivery of targeted strategies or supports early in the claims process.
The effect of advanced recovery room care on postoperative outcomes in moderate‐risk surgical patients: a multicentre feasibility study
Authors: Ludbrook G. , Lloyd C. , Story D. , Maddern G. , Riedel B. , Richardson I. , Scott D. , Louise J. , Edwards S. 
Published: October 2020

SummaryPostoperative complications are common and may be under‐recognised. It has been suggested that enhanced postoperative care in the recovery room may reduce in‐hospital complications in moderate‐ and high‐risk surgical patients. We investigated the feasibility of providing advanced recovery room care for 12–18 h postoperatively in the post‐anaesthesia care unit. The primary hypothesis was that a clinical trial of advanced recovery room care was feasible. The secondary hypothesis was that this model may have a sustained impact on postoperative in‐hospital and post‐discharge events. This was a multicentre, prospective, feasibility before‐and‐after trial of moderate‐risk patients (predicted 30‐day mortality of 1–4%) undergoing non‐cardiac surgery and who were scheduled for postoperative ward care. Patients were managed using defined assessment checklists and goals of care in an advanced recovery room care setting in the immediate postoperative period. This utilised existing post‐anaesthesia care unit infrastructure and staffing, but extended care until the morning of the first postoperative day. The advanced recovery room care trial was deemed feasible, as defined by the recruitment and per protocol management of > 120 patients. However, in a specialised cancer centre, recruitment was slow due to low rates of eligibility according to narrow inclusion criteria. At a rural site, advanced recovery room care could not be commenced due to logistical issues in establishing a new model of care. A definitive randomised controlled trial of advanced recovery room care appears feasible and, based on the indicative data on outcomes, we believe this is warranted.