bluuebunny/crossref_metadata_2025_split huggingface dataset:
Entrenamiento de balonmano en las categorías junior y senior: diferencias y similitudes
Authors: Pombo Menezes R. , Longarela Pérez B. , Rafaela Galatti L. 
Published: July 2020

Entrenadores de balonmano buscan el desarrollo de diferentes capacidades de los jugadores, a través de diferentes métodos de enseñanza. El objetivo del presente estudio fue identificar las diferencias y similitudes metodológicas en el proceso de entrenamiento de las categorías júnior y sénior. Se entrevistaron seis entrenadores de balonmano y las entrevistas fueron analizadas con el método Discurso del Sujeto Colectivo. Los entrenadores mencionaron características similares en las categorías: el aumento de la especificidad y la obtención de resultados a partir de la categoría júnior; y como principales diferencias: las experiencias competitivas y la búsqueda de resultados. Los entrenadores señalaron que utilizan métodos de enseñanza similares en ambas categorías, principalmente por entrenaren en conjunto y por la necesidad de obtener resultados. Aunque los entrenadores creen que es necesario que las jugadoras tengan más experiencias relacionadas con el ambiente competitivo, hay un reconocimiento hacia los contenidos con los que tuvieron contacto durante la formación deportiva.
Handball coaches aim to develop different players’ skills, using different teaching approaches. The aim of this work was to identify the differences and methodological proximity between the junior and adult teams of women's handball. Six handball coaches were interviewed and the speeches were analyzed using the Collective Subject Discourse method. The coaches mentioned similar characteristics of both teams, and point out the increase in specificity and results collection from the junior team; and the main differences between these teams are related to the competitive experiences and the magnitude of the results charged. Coaches have been using similar teaching approaches mainly because the integration between the teams and the search for expressive results in competitions. Although coaches believe that there is a need for more competitive experiences for women players, there is a charge for the content with which they are supposed to have contact throughout their entire training process.
Modeling ratios of phenotypic classes in wheat hybrids of introgressive origin for glume color trait
Authors: Antonyuk M. Z. , Iefimenko T. S. , Navalikhina A. G. , Ternovska T. K. 
Published: July 2020

Aim. Develop inheritance model for glume color in hybrid generations considering introgressive origin of crossing components. Methods. Phenotypic assessment of the trait, cytological study of chromosome conjugation in meiosis, methods determining statistical significance of the results. Results. Twelve cross combinations of wheat introgressive with one another and with recurrent genotype Aurora were studied. Selected for the crosses introgressive lines had contrasting glume color trait. Characteristics of wheat introgressive lines and its hybrids which would be important in hybrid analysis for the development of the inheritance model were identified. Genetic analysis of the introgressive lines was done as for the number of glume color genes for which lines differed from one another and from the Aurora genotype. Conclusions. In case the critical gene in localized in a chromosome that do not conjugate with hom(e)olog of the cross partner, F1 hybrids developed with introgressive lines form gametes and zygotes with frequencies differing from theoretically expected for the cross of homozygous lines.
Keywords: wheat introgression lines, Aegilops, glume colour, aneuploidy gametes and zygotes, meiotic drive.
Choice lines of Drosophila melanogaster with reporter system sensitive to low doses of mutagens
Authors: Yasinskyi Ya. S. , Omelianchuk L. V. , Zhuk O. V. , Kozeretska I. A. 
Published: July 2020

Aim. There are many methods of assessment of mutagens, but the effects of low-dose mutagens have been overlooked. In this study we try to select lines of Drosophila melanogaster with high-sensitive to mutagen reporter gene. We used D. melanogaster lines with enhancer trap P {lacW} which produces beta-galactosidase gene in response to activation induced reparation. Methods. Ionizing radiation in dose of 100 roentgens were used as a mutagen. We extracted salivary glands of the third instar of the fruit fly larvae. The sample was fixed with 0.75 % glutaraldehyde and stained by 12-hour incubation with 0.2 % X-Gal at 37°C. Activity of the reporter gene was determined by microscope. Results. Two of the three lines are not shown according to the activation of the reporter gene from the resulting dose of ionizing radiation. In line y [1] w [67c23]; P {w [+ mC] = lacW} mus209 [k00704] / CyO (#10361) activation of the reporter gene occurred in some nuclei salivary gland after radiation. Conclusions. The reporter gene P{lacW} may be used for the detection of small doses of mutagens. Larvae of line #10361 has reporter gene activation in response to ionizing radiation.
Keywords: mutagenic factors, Drosophila melanogaster, LacZ reporter, radiation.
Use of chornobyl mutants’ genetic instability in breeding process of Triticum aestivum. L. for adaptability
Authors: Burdeniuk-Tarasevych A. L. 
Published: July 2020

Aim. The results of 30-year-long study of T. aestivum L. winter wheat hereditary changes that took place in result of radiation exposure in the fields near the destroyed Chernobyl reactor during two year period (1986–1987) were provided. Methods. The modified forms of plants were cultivated by pedigree method for 30 years. Results. The following types of mutations were found: 1) morphological – ear, spikelet’s, stems, leaves structure anomalies; 2) systemic that featured traits of other species – T. spelta (L); T. compactum (Host) and T. vavilovi (Tum. and Jakubz); 3) physiological growth and development mutations accompanied by hormonal systems changes, sterile flowers, early and late ma-turing; dwarfness and height change; 4) mutations of stress resistance, improved winter hardiness, drought tolerance and resistance to diseases. Conclusions. Mutations were genetically unstable and their range extended from generation to generation. Mutations on high adaptability manifested themselves only under stressful environmental conditions. They were used as a starting material for varieties that were included into the State Register. This way Yasochka variety had increased drought tolerance and resistance to lodging; Lybid variety had increased resistance to ice crust; Tsarivna, Romantica and Lisova Pisnia varieties – high winter hardiness and drought tolerance; Vidrada – organic production suitability with resistance to main diseases and a superb baking qualities.
Keywords: variety, wheat, unstable mutagenesis, adaptability.
Comparison of groups of Triticum aestivum L. varieties of the Steppe and the Forest-Steppe of Ukraine by markers for important genes
Authors: Sozinov I. O. , Kozub N. O. , Karelov A. V. , Pylypenko L. A. , Bidnyk H. Ya. , Demianova N. O. , Blume Ya. B. , Sozinov O. O. 
Published: July 2020

Aim. The aim of the study was to compare diversity of groups of winter common wheat varieties of the Steppe and the Central Forest-Steppe of Ukraine by storage protein loci and some disease resistance genes and to reveal peculiarities of varieties released after 1995. Methods. SDS and APAG electrophoresis was used to identify genotypes at the Glu-1, Gli-1, and some minor gliadin loci. PCR analysis was employed to study alleles of the disease resistance genes Lr34/Yr18/Pm38/Sr57/Bdv1, Tsn1, Tsc2, TDF_076_2D, and Cre-8. Results. Significant differences in frequencies of alleles at most marker loci were revealed. Nonrandom associations between disease resistance gene alleles as well as storage protein alleles were detected. Conclusions. The retention of a set of predominant alleles of a certain zone in different periods of breeding was confirmed. The appearance of new allele associations in the groups of varieties of the Steppe (in particular Gli-A1g and Glu-B1al) and the Central Forest-Steppe (1AL/1RS and Glu-B1d) in the last two decades was noted.
Keywords: Triticum aestivum L., varieties, storage proteins, resistance genes, alleles.
Microsatellite locus Ame17 polymorphism for round goby (Neogobius melanostomus) from Northwest Black Sea region
Authors: Mishchuk Ya. M. , Serga S. V. , Radionov D. B. , Zamorov V. V. , Chuiko V. Yu. , Chebotar S. V. , Kozeretska I. A. 
Published: July 2020

Aim. To identify the Ame17 polymorphic variants for round goby Neogobius melanostomus from Northwest Black Sea region. Methods. We determined polymorphism for 102 individuals of round goby. Genotyping was performed by PCR-analysis of Ame17 as described by Brown et al., 2008. The PCR-amplified DNA products were subjected to electrophoresis in 8 % polyacrylamide gel. Results. There have been identified 23 alleles. The range of repeat sizes was from 140 to 300 bp. The average heterozygosity was 0.76. Conclusions. There have been analyzed polymorphisms of Ame17 microsatellite locus for round goby from Northwest Black Sea region. The highest genetic diversity at Ame17 locus was observed for fish from the Gulf of Odessa. In terms of heterozygosity subpopulation of round goby from Bay Dzharylgach was significantly different from other localities.
Keywords: round goby, polymorphism, microsatellite loci, Ame17.
The combining ability of poltava meat and welsh pigs breed combination
Authors: Tsereniuk O. M. , Akimov O. V. , Chalyi O. I. , Chereuta Yu. V. 
Published: July 2020

Aim. Poltava Meat and Welsh pigs breed reciprocal crossing combination ability study. Methods. The combination ability evaluation method, the method of incomplete diallel crossing. The traditional methods of pigs general and specific combining ability effects estimation. Results. The Rosinka family sows were characterized by positive values of general combining ability, except the prolificacy rate. The Bystra family sows by the multiple pregnancy and milk production indexes had positive values of general combining ability, but by a nest weight at a birth and weaning – negative. For the Vorskla family sows the positive indexes of general combining ability were identified for a nest weight at a birth only, for the rest indexes – the negative value of general combining ability were installed. The Rosinka family sows had the positive value of specific combining ability for all maternal productivity indxes. The Bystra family sows were characterized by positive value of specific combining ability only for a nest weight at a birth and milk production indexes. The Vorskla family sows by the all maternal productivity indexes had negative specific combining values. Conclusions. For complex reproductive ability indexes for various lines and families combinations the highest positive effect of specific combining values was obtained for Rosinka family sows combinations of Poltava meat breed with the Welsh breed boars.
Keywords: pigs, Welsh breed, Poltava Meat breed, reproductive ability, combining ability.
Genetic profiling of pathogenic for apple tree Erwinia sp. strains
Authors: Dankevych L. A. 
Published: July 2020

Aim. It is known, that significant damage to pome fruit causing representatives of phylogenetically closely related species Erwinia amylovora, E. pyrifoliae, E. piriflorinigrans, E. uzenensis. That is why, for their correct identification using molecular genetic techniques, including REP-PCR, which can not only correctly identify the pathogen, and evaluate the heterogeneity of its population. In this regard, the aim of our research was genome fingerprinting of isolated strains and typical Erwinia amylovora UCM B 1095T strain, using REP-PCR, for their correct taxonomy at the species level. Methods. Genetic profiling of isolated Erwinia sp. strains and the typical Erwinia amylovora B 1095T strain carried out by REP-PCR. Results. As a result of REP-PCR analysis revealed a significant relationship of isolated Erwinia sp. strains and the typical Erwinia amylovora UCM B 1095T strain. Conclusions. As a result, BOX, REP and ERIC profiling set low genetic heterogeneity of isolated Erwinia sp. strains and their great affinity with the typical Erwinia amylovora UCM B 1095T strain.
Keywords: REP-PCR, isolated Erwinia sp. strains, Erwinia amylovora, pome fruit culture.
Molecular genetics for sports medicine
Authors: Mosse I. B. , Kilchevskyi A. V. , Morozyk P. M. , Honchar A. L. , Zhur K. V. 
Published: July 2020

Aim. To develop and test a molecular-genetic technology for evaluation of a genetic predisposition to thrombogenic diseases, bone fractures and emotional disruptions resulting from physical and emotional loads. Methods. Molecular genetic analysis was carried out by PCR. Polymorphisms of F2 and F5 genes were analyzed to assess the risk of thrombus formation. Polymorphisms of VDR, COL1A1 and COL1A2 genes were analyzed to determine a bone fracture risk. Genetic stress resistance was identified by genes of dopaminergic system (DRD2, DRD4) and serotoninergic system (HTR2A, 5-HTTVNTR and 5-HTTLPR). The HADS, Maslach and Boyko psychological tests were used. Results. It was revealed that 4.3 % of the tested athletes are carriers of Leiden mutation and 2.2 % – of prothrombin gene mutation. It was shown that polymorphisms ApaI and BsmI of VDR gene are the most informative for bone fracture risk detection. Some variants and combinations of genes were shown to be the most informative for a stress-resistance identification. Conclusions. The technology for risk evaluation of different pathologies resulting from physical and emotional loads has been developed and tested.
Keywords: sport, stress-resistance, bone fractures, gene polymorphisms, PCR analysis.
Evaluation of Teacher Digital Competence Frameworks Through Expert Judgement: the Use of the Expert Competence Coefficient
Authors: Cabero-Almenara Julio , Romero-Tena Rosalía , Palacios-Rodríguez Antonio 
Published: July 2020

Abstract
In this paper, different proposals made by national and international organizations and institutions on Teacher Digital Literacy are taken into account. The 7 most commonly used competence frameworks in international contexts are presented for evaluation through expert judgment. Next, the most suitable for the subsequent realization of a t-MOOC on Teacher Digital Literacy was selected. The selection of these experts was based on seven criteria that helped to establish the expert knowledge coefficient (EKC). This strategy served to obtain the viability of the selected sample. A total of 412 people, 155 experts and 257 non-experts participated in this study. After conducting the expert judgment, in a single round, it was concluded that the European Framework of Digital Competence for Teachers DigCompEdu is the most valued and adequate to be used as reference for the formative t-MOOC, followed by INTEF. This result is logical, since the latter is inspired by the former. These findings do not question the validity of the other competency frameworks, but the preferences granted by the judges.
Digital Competence: A Study from the Perspective of Pre-service Teachers in Turkey
Authors: Çebi Ayça , Reisoğlu İlknur 
Published: July 2020

Abstract
This study aims to know the opinions of pre-service teachers on their digital competence and to determine whether these opinions vary according to gender, branch and perceived level of digital competence. In this study, a cross-sectional survey model was used. In such a context, the study was conducted with 518 pre-service teachers who were studying in different provinces of Turkey. The study used a digital competence questionnaire as a data collection tool. When the results were evaluated, it can be said that the digital competence of pre-service teachers is moderate and that it varies significantly according to gender, branch and perceived level of digital competence. It is thought that the results of this study will guide the researchers as they reveal the needs of the pre-service teachers and contain information about what should be focused on the training or activities to be organized to meet these needs.
Development of an Eco-material Based on Palmyra Aggregates
Authors: Doko Valéry K. , Chabi Edem , Hounkpè Sena Peace , Adjovi Edmond C. 
Published: July 2020

This study is part of a project to develop a bio-based material meeting the criteria of sustainable development in the field of construction. A study was then made on a cement matrix composite reinforced by aggregates of Borassus aethopium mart (palmyra).
For this purpose, studies of cement compatibility with this plant aggregate, the physical, mechanical and thermal behavior of the concrete were performed for two granular compositions. The compatibility study revealed that palmyra aggregrates, although not previously treated, are compatible with Portland-limestone cement. The characterization tests focused on the mechanical properties of three-point flexural tensile strength and compressive strength and thermal properties such as effusivity, conductivity and diffusivity. The results obtained are promising: they have very good mechanical and thermal characteristics, considering its use in construction. The mechanical strengths reach 16.25 MPa for a density less than 1500 kg / m3. The thermal conductivity varies between 0.270 and 0.415. They can be used for the manufacture of panels used in the realization of suspended ceilings and tiles for the realization of roofs.
Human Papilloma Virus Transmission: Knowledge and Uptake of HPV Vaccine among In-school Adolescent Girls in South-South Nigeria
Authors: Ojule I. N. , Anika I. E. 
Published: July 2020

Background: HPV infection is the most common STI in sexually active adolescents. It has been implicated in majority of cases of cervical cancers. HPV is preventable. Potent Vaccines are available.
Objective: This study assessed what adolescents know about HPV infection and HPV vaccination, their attitude and uptake of HPV vaccine. This was also to raise awareness and obtain data that will be useful in identifying where to intervene to improve coverage.
Materials and Methods: A descriptive cross-sectional study carried out in Rivers State, Nigeria. Study tool was a semi-structured, self-administered questionnaire. Multi-stage sampling method was used to recruit adolescent girls from secondary schools.
Results: 445 in-school adolescent girls aged 9 to 19 years participated. Mean age was 13.4 SD = ±2.2 years. 36.6% and 36.8% had heard of HPV infection and HPV vaccine respectively. Only 3.1% of the participants knew HPV could be prevented through vaccination. 71.7% indicated willingness to obtain the vaccine. Only 3.6% self reported to have received at least one dose of the vaccine at the time of the survey. Uptake of HPV vaccine (P=0.00), willingness to be vaccinated (P=0.005) highest among adolescents that had heard of HPV vaccine and those who perceived themselves to be at risk for HPV infection (P=0.005). Insufficient health information, cost of HPV vaccines identified as key barriers to vaccines utilization.
Conclusion: Notwithstanding the low level of knowledge about HPV infection and HPV vaccines willingness to be vaccinated was high. Our study shows that uptake of vaccination is low in our locality.
Serum long non‐coding RNA LINC00887 as a potential biomarker for diagnosis of renal cell carcinoma
Authors: Xie Junjie , Zhong Yu , Chen Rong , Li Gang , Luo Yongwen , Yang Jintao , Sun Zhongwei , Liu Yanzhong , Liu Peipei , Wang Na , An Jiaqi , Li Chao , Song Yang 
Published: July 2020

The identification of non‐invasive biomarkers for the detection of renal cell carcinoma (RCC) in early‐stage patients may help improve disease outcome. Certain long non‐coding RNAs (lncRNAs) have been reported to be possible biomarkers for the diagnosis and prognosis of cancer. Here, we examined the suitability of the lncRNA LINC00887 as a potential biomarker for RCC because its expression has been shown to be elevated in RCC tissue versus normal tissue in the Gene Expression Profiling Interactive Analysis (GEPIA) database. We found that LINC00887 expression is significantly increased in early‐stage RCC tissues and the serum of early‐stage RCC patients compared to matched normal tissues and the serum of healthy subjects, respectively. We also demonstrated that elevated serum LINC00887 is generated from the tumor tissues of RCC patients. Moreover, a receiver operating characteristic (ROC) curve was generated to analyze the diagnostic value of serum LINC00887. The area under the ROC cure differentiating early‐stage RCC patients from healthy subjects was 0.8001, with a sensitivity of 71.05% and a specificity of 89.87%. Furthermore, we found that LINC00887 promotes RCC cell proliferation in vitro. Taken together, our findings suggest that a serum LINC00887 signature is associated with RCC cell proliferation and may be a potential biomarker for the detection of early‐stage RCC.
“EMERGing” Electronic Health Record Data Metrics: Insights and Implications for Assessing Residents’ Clinical Performance in Emergency Medicine
Authors: Sebok‐Syer Stefanie S. , Shepherd Lisa , McConnell Allison , Dukelow Adam M. , Sedran Robert , Lingard Lorelei 
Published: July 2020

AbstractObjectivesCompetency‐based medical education requires that residents are provided with frequent opportunities to demonstrate competence as well as receive effective feedback about their clinical performance. To meet this goal, we investigated how data collected by the electronic health record (EHR) might be used to assess emergency medicine (EM) residents’ independent and interdependent clinical performance and how such information could be represented in an EM resident report card.MethodsFollowing constructivist grounded theory methodology, individual semistructured interviews were conducted in 2017 with 10 EM faculty and 11 EM residents across all 5 postgraduate years. In addition to open‐ended questions, participants were presented with an emerging list of EM practice metrics and asked to comment on how valuable each would be in assessing resident performance. Additionally, we asked participants the extent to which each metric captured independent or interdependent performance. Data collection and analysis were iterative; analysis employed constant comparative inductive methods.ResultsParticipants refined and eliminated metrics as well as added new metrics specific to the assessment of EM residents (e.g., time between signup and first orders). These clinical practice metrics based on data from our EHR database were organized along a spectrum of independent/interdependent performance. We conclude with discussions about the relationship among these metrics, issues in interpretation, and implications of using EHR for assessment purposes.ConclusionsOur findings document a systematic approach for developing EM resident assessments, based on EHR data, which incorporate the perspectives of both clinical faculty and residents. Our work has important implications for capturing residents’ contributions to clinical performances and distinguishing between independent and interdependent metrics in collaborative workplace‐based settings.