bluuebunny/crossref_metadata_2025_split huggingface dataset:
Level and timing of Implanon discontinuation and associated factors among women who used Implanon in Andabet District, public health facilities, North-West Ethiopia
Authors: Dagnew Gizachew Worku , Asresie Melash Belachew 
Published: November 2020

Abstract
Background: Implanon discontinuation is unacceptably high in developing countries, including Ethiopia. Furthermore, there is an observed problem of high unintended pregnancy after method discontinuation that stride to program failure. Therefore, the purpose of this study was to assess the level and determinants of Implanon discontinuation among women who used Implanon in Andabet district, public health facilities, North-West Ethiopia, 2017.Methods: Facility-based cross-sectional study design was employed among 537 women from Feb.03 to April 28, 2017. Study participants were selected using a systematic random sampling technique. A face-to-face interview was employed to collect data. Epi Info- version-7 was used for data entry and SPSS version 20 for analysis. Both descriptive and analytical statistical analysis was computed. On multi-variable binary logistics regression, a p-value of less than 0.05 was used to declare statistical significance.Results: About 37% of Implanon users have discontinued the method before the intended time. Of about 86% of them were discontinued Implanon before two years of insertion. Women who had no live child [AOR=2.17,95%CI:1.25-3.77], women who didn’t receive pre-insertion counseling [AOR=1.85,95%CI:1.15-2.97], women who developed Implanon related side effect [AOR=5.17,95%CI:3.18-8.40], and women who did not satisfy by the service provided [AOR=5.40,95%CI:3.04-9.57] had higher odds of Implanon discontinuation. On the other hand, women who received appointment follow-up [AOR=0.23,95%CI:0.13-0.41] had lower odds of Implanon discontinuation.Conclusions: The level of Implanon discontinuation before its intended time was high in the district. Hence, strengthening pre-insertion counseling and appointment follow-up as well as improving the client’s level of service satisfaction may increase Implanon continuation.
MSRD: Multimodal Web Rumor Detection Method
Authors: Liu Jinshuo , Feng Kuo , Pan Jeff Z , Deng Juan , Wang Lina 
Published: November 2020

Abstract
Multimodal web rumors, which combine images and text, are confusing and can be inflammatory, and therefore can be harmful to national security and social stability. Currently, web rumor detection fully considers text content but ignores image content, including text embedded in images. This paper proposes a multimodal web rumor detection method based on a deep neural network considering images, image-embedded text, and text content. This method uses a VGG-19 network to extract image content features, DenseNet to extract embedded text content, and an LSTM (Long Short-term Memory) network to extract text content features. After concatenation with image features, the mean and variance vectors of the image and text shared representations are obtained through a completely connected layer, and random variables sampled from a Gaussian distribution are used to form a reparameterized multimodal feature as the input of the rumor detector. Experiments show that the accuracy of this method is 68.5% and 79.4% on Twitter and Weibo, respectively.
The Classification of Public Complaints through Smart City Application
Authors: Madyatmadja Evaristus Didik , Wijaya Cristofer 
Published: November 2020

Abstract
This research aimed to classify the data of public complaints of people in Tangerang City by applying a pattern of the complaint data from the LAKSA application that has been categorized. In finding the pattern, it used one of the data mining methods, namely classification. The classification algorithm search process was performed by comparing the accuracy of several selected algorithms. The algorithms were k-nearest neighbor, random forest, support vector machine, and AdaBoost. These algorithms were tested to achieve maximum potential. Thus, the results showed support vector machine with linear kernel is a classification algorithm with the highest accuracy that reached 89.2%
The lateral periaqueductal gray and its role in controlling predatory hunting and social defense.
Authors: Marín-Blasco Ignacio , Rangel Miguel , Baldo Marcus , Motta Simone , Stowers Lisa , Canteras Newton 
Published: November 2020

Abstract
Evasion from imminent threats and prey attack are opposite behavioral choices critical to survival. The lateral periaqueductal gray (LPAG) is a key player in these behaviors, it responds to social threats and prey hunting while also driving predatory attacks and active defense. Our results revealed that distinct neuronal populations in the LPAG drive prey hunting and evasion from social threats. We show that the LPAG provides a putative glutamatergic projection to the lateral hypothalamic area (LHA). LPAG > LHA pathway optogenetic inhibition impaired insect predation but did not alter escape/attack ratio during social defeat. The results suggest that the LPAG control over evasion to a social attack may be regarded as a stereotyped response depending probably on descending projections. Conversely, the LPAG control over predatory behavior involves an ascending pathway to the LHA that likely influences LHAGABA neurons driving predatory hunting and may provide an emotional drive for appetitive rewards.
The Relationship Between Modified Nordic Diet and Resting Metabolic Rate Among Overweight and Obese Participants: A Cross Sectional Study
Authors: Khalili Ghazaleh , Mirzababaei Atieh , Shiraseb Farideh , Mirzaei Khadijeh - 
Published: November 2020

Abstract
Objective: Obesity as a worldwide phenomenon is a multifactorial condition. Healthy diets have effect on obesity related factors like resting metabolic rate (RMR). In present study, we investigate association between adherence to modified Nordic diet and RMR among overweight and obese participants.Methods: We enrolled 404 overweight and obese (BMI ≥25 kg/m2) women aged 18-48 years in this cross-sectional study. For each participant anthropometrics measurements, biochemical tests and blood pressure were evaluated. RMR was measured by indirect calorimetry. RMR/kg was also measured. Modified Nordic diet score was measured using a validated 147-item food frequency questionnaire (FFQ).Results: Among all participants, the mean and standard deviation (SD) for age and body mass index (BMI) were 36.67 years (SD=9.10) and 31.26 kg/m2 (SD=4.29). There was a significant association between RMR/kg status and age, body mass index (BMI), RMR (P<0.001), respiratory quotient (RQ), fat percentage (P= 0.01), systolic blood pressure (SBP) (P= 0.03), and diastolic blood pressure (DBP) (P= 0.04), after adjustment for age, BMI, energy intake and physical activity. Participants with the highest adherence to modified Nordic diet had lower odds of hypometabolic status after adjusting for confounders and it was significant (odds ratio (OR) = 3.15, 95% CI= 0.97-10.15, P=0.05).Conclusions: The present results indicate that adherence to modified Nordic diet is associated with lower odds of hypometabolic status in overweight and obese women. However more studies are needed to confirm our findings.Trial registration: This article was not a trial research.
Gut microbiome alterations predict diabetic kidney disease in general population
Authors: Zhao Zhanzheng , Zhang Weifeng , Guo Ruixue , Cui Wen , Zhang Fanliang , Geng Yingyang , Liu Chao , Shang Jin , Xiao Jing , Wen Xuejun 
Published: November 2020

Abstract
Background

Diabetic kidney disease (DKD) is increasingly prevalent owing to worldwide epidemic of diabetes mellitus (DM). The relationship between gut microbiome and chronic kidney disease (CKD) has attached wide attention during recent years. While the role of gut microbiome in diagnosis of DKD remains unclear.
Methods

We collected fecal samples of 180 patients with DKD and 179 healthy controls (Con) and characterized microbial profile using 16S rRNA gene sequencing approach. Microbial communities between the two groups were compared by Wilcoxon test. Two microbial models were identified by cross-validation preformed on random forest analysis. Diagnostic value of the two models was assessed in the training set including 118 DKDs and 132 healthy controls and validated in testing set composed of 62 DKDs and 47 healthy controls. PICRUSt was exploited to compare the potential metabolic function of microbiota between two groups.
Results

Compared with healthy controls, bacterial diversity in DKDs was decreased. Beta diversity showed markedly separating distribution between DKDs and Con groups. At the phylum level, DKD patients showed higher proportion of Proteobacteria, Verrucomicrobia and lower proportion of Bacteroidetes. In more details at the genus level, increase of opportunistic pathogens (including Escherichia-Shigella, Klebsiella, Enterococcus, Erysipelotrichaceae_incertae_sedis, Peptostreptococcaceae_incertae_sedis, Streptococcus) and mucin-degrading bacteria (including Akkermansia, Lactobacillus),as well as reduction of bacteria producing short-chain fatty acids (SCFA) such as Blautia༌Faecalibacterium were also observed in DKDs. The optimal prediction model consisting of 5 OTUs could distinguish DKD from healthy controls with an area under the ROC curve of 83.71% in training set and 81.56% in testing set, while another 22 OTUs couldn’t significantly improve diagnostic efficiency. Genomic metabolism associated with lipid and aromatic amino acids was over-expressed in DKD vs. Con.
Conclusion

Gut microbiome was altered in DKD patients. Our study suggested that gut microbiome may provide a potential tool for DKD diagnosis, especially those who could not accept or afford renal biopsy.
Abnormal Anther Development Leads to Lower Spikelet Fertility in Rice (Oryza Sativa L.) Under High Temperature During the Panicle Initiation Stage
Authors: Hu Qiuqian , Cui Kehui , Wang Wencheng , Lu Qifan , Huang Jianliang , Peng Shaobing 
Published: November 2020

Abstract
Background: Decreased spikelet fertility is often responsible for reduction in grain yield in rice (Oryza sativa L.). In this study, two varieties with different levels of heat tolerance, Liangyoupeijiu (LYPJ, heat susceptible) and Shanyou63 (SY63, heat tolerant) were subjected to two temperature treatments for 28 days during the panicle initiation stage in temperature/relative humidity-controlled greenhouses: high temperature (HT; 37/27℃; day/night) and control temperature (CK; 31/27℃; day/night) to investigate changes in anther development under HT during panicle initiation and their relationship with spikelet fertility.Results: HT significantly decreased the grain yield of LYPJ by decreasing the number of spikelets per panicle and seed setting percentage. In addition, HT produced minor adverse effects in SY63. The decreased spikelet fertility was primarily attributed to decreased pollen viability and anther dehiscence, as well as poor pollen shedding of the anthers of LYPJ under HT. HT resulted in abnormal anther development (fewer vacuolated microspores, un-degraded tapetum, unevenly distributed Ubisch bodies) and malformation of pollen (obscure outline of the pollen exine with a collapsed bacula, disordered tectum, and no nexine of the pollen walls, uneven sporopollenin deposition on the surface of pollen grains) in LYPJ, which may have lowered pollen viability. Additionally, HT produced a compact knitted anther cuticle structure of the epidermis, an un-degraded septum, a thickened anther wall, unevenly distributed Ubisch bodies, and inhibition of the confluent locule, and these malformed structures may be partially responsible for the decreased anther dehiscence rate and reduced pollen shedding of the anthers in LYPJ. In contrast, the anther wall and pollen development of SY63 were not substantially changed under HT. Conclusions: Our results suggest that disturbed anther walls and pollen development are responsible for the reduced spikelet fertility and grain yield of the tested heat susceptible variety, and noninvasive anthers and pollen formation in response to HT were associated with improved heat tolerance.
The Motor Neuron Disease Mouse Model hSOD1-G93A Presents a Non-canonical Profile of Senescence Biomarkers in The Spinal Cord
Authors: Torres Pascual , Anerillas Carlos , Encinas Mario , Povedano Monica , Andrés-Benito Pol , Ferrer Isidro , Ayala Victòria , Pamplona Reinald , Portero-Otin Manuel 
Published: November 2020

Abstract
Recent evidence demonstrates a pathological role for senescent cells in Alzheimer’s and Parkinson’s diseases. The present study aimed to show senescence mechanisms including senescence-associated secretory phenotype (SASP) in the familial amyotrophic lateral sclerosis (ALS) transgenic mouse model hSOD1-G93A. We evaluated, as senescence biomarkers, the expression of p16 and p21 with reverse-transcriptase quantitative PCR (RT-qPCR), immunofluorescence (IF), and immunohistochemistry (IHC), as well as the senescence-associated β galactosidase (SA-β-gal) activity in the lumbar spinal cords (LSC) of this model. As SASP markers, we quantified the mRNA levels of Il1a, Il6, Ifna, and Ifnb. Furthermore, we explored if an alteration of alternative splicing is associated with senescence phenomena in this model. Thus, we quantified the Adipor2 cryptic exon inclusion levels, a specific splicing variant repressed by TAR-DNA binding of 43 kDa (TDP-43), using RT-qPCR. Our results show an atypical senescence-profile in LSC from transgenic mice, increasing p16 and p21 mRNA and protein levels in glial cells with a mostly cytoplasmic pattern, without the canonical increase in SA-beta-gal activity in these cells. Consistent with enhanced SASP, there is an increase in Il1a and Il6 expression. Also, TDP-43 splicing activity is compromised in this ALS model, in a direct relationship with the increase in p16 expression. However, senolytic drug Navitoclax -with reported benefits in Alzheimer and Parkinson disease mouse models - does not alter the present model’s disease progression. Navitoclax neither eliminates cells expressing senescence and nor represses the expression of SASP related genes. Globally, our findings support the existence of a non-canonical profile of senescence biomarkers in the LSC of the ALS model hSOD1-G93A.
Full neuroendoscopic contralateral longitudinal fissure approach to resection of parafalcine meningioma via falx cerebri – Retractorless surgical technique: a case report
Authors: Hu Changchen , Liu Jiangtao , Ji Hongming , Zhang Gangli , Chen Shengli , Zhang Shiyuan , Wang Kaixuan 
Published: November 2020

Abstract
Background: Parafalcine meningioma often invades into contralateral hemisphere, and sometimes it can develop to both sides. An ipsilateral craniotomy is generally performed for the surgical resection of parafalcine meningioma.Case report: Here, we report that a 50-year-old female with a right anterior cerebral parafalcine meningioma. The patient was in supine position and the head was rotated 20 degrees to the opposite side. A neuronavigation was employed to perform a precise surface localization of parafalcine meningioma. Under neuroendoscopy, a contralateral craniotomy and a longitudinal fissure approach were followed by an incision of dura to expose arachnoid, then arachnoid was separated until falx cerebri along its surface. Neuronavigation was employed again to precisely locate the projection of meningioma in the contralateral falx cerebri. According to the position, firstly removed the invaded falx cerebri, blocked the blood supply of the meningioma, then completely resected the meningioma without retraction of normal brain tissue.Conclusion: We conclude that full neuroendoscopic contralateral longitudinal fissure approach to complete resect parafalcine meningioma via falx cerebri using retractorless surgical technique can reduce the retraction of normal brain tissue to enhance protection of brain function, while maximizing the total resection rate of parafalcine meningioma.
Evaluation of Malaria Prevalence among Patients Attending in Bibugn district, East Gojjam Zone, Ethiopia
Authors: Tsega Anmut Assemie , Abate Getent Atenafu 
Published: November 2020

Abstract
Malaria is an important public health problem in Ethiopia, with more than 54 million people at risk of infection. Plasmodium falciparum and P. vivax are both present. The general objective of the study was to determine the prevalence of malaria and associated parasites through microscopic examination of blood films. Cross-sectional study was carried in the study sites. The study was conducted from October to December 2017 and March to April 2018. Out of 822 study participants, 46(5.6%) were malaria positive. Of these, 28(60.87%) were P.vivax and 18(39.13%) P.falciparum. The prevalence of parasites was significantly greater in rural villages (6.25%) than urban village (3.23%) More males were infected compared to females but not significantly Plasmodium vivax was higher in prevalence than P. falciparum in the study area. So the result contrast the national figure of malaria report. The findings of this study may be useful for those who work in malaria control and prevention program.
GenoVault: A Cloud Based Genomics Repository
Authors: Jain Sankalp , Saxena Amit , Hesarur Suprit , Bhadhadhara Kirti , Bharti Neeraj , Kasibhatla Sunitha Manjari , Sonavane Uddhavesh , Joshi Rajendra 
Published: November 2020

Abstract
GenoVault is a cloud-based repository for handling Next Generation Sequencing (NGS) data. It is developed using OpenStack based private cloud with various services like keystone for authentication, cinder for block storage, neutron for networking and nova for managing compute instances for the Cloud. GenoVault uses object-based storage, which enables data to be stored as objects instead of files or blocks for faster retrieval from different distributed object nodes. Along with a web-based interface JavaFX-based desktop client has also been developed to meet the requirements of large file uploads ( > 5 GB) that is usually seen in NGS datasets. Users can store as many as one million files in their respective object based storage areas and the metadata provided by the user during file uploads is used for querying the database. GenoVault repository is designed taking into account future needs and hence can scale both vertically and horizontally without any need for modification in the design. Users have an option to make the data shareable to the public or restrict the access as private. Data security is ensured as every container is a separate entity in object-based storage architecture also supported by secured file transfer protocol during data upload and download. The data is uploaded by the user in their individual containers that include raw read files (fastq), processed alignment files (bam, sam, bed) and output of variation detection (vcf). GenoVault architecture allows verification of the data in terms of integrity and authentication before making it available to collaborators as per user permissions. GenoVault is useful for maintaining the organization wide NGS data generated by experiments in various labs which is not yet published and submitted to public repositories like NCBI. GenoVault also provides support to share NGS data among the collaborating institutions. GenoVault can thus manage vast volumes of NGS data on any OpenStack-based private cloud.
Area-restricted search in Magellanic Woodpeckers: importance of tree senescence, forest composition and open habitats
Authors: Vergara Pablo , Maron-Perez Francisca , Caro Diego , Soto Gerardo E , Simonetti Javier A 
Published: November 2020

Abstract
Background
Large woodpecker species with stable territories, specialized diets and narrow habitat choices, such as the Magellanic Woodpecker (Campephilus magellanicus), are expected to adjust their movements based on the distribution of available resources within territories. Thus, Magellanic Woodpeckers should concentrate foraging activity in areas of higher quality, a behavior consistent with the area-restricted search (ARS) behavior. Although previous studies have deepened the understanding of how Magellanic woodpeckers select habitats, the assessment of ARS behavior would contribute to the identification of feeding areas important for their conservation and habitats hindering their movement.
Methods
We addressed the independent effects of tree senescence, forest succession, stand composition and open habitats on the adoption of area-restricted search (ARS) behavior in Magellanic Woodpeckers in a heterogeneous landscape dominated by southern beech forest in southern South America. Using GPS relocations from 24 woodpeckers, we estimated the First-Passage Time (FPT), a measure of the time individuals remain in a given area, with longer FPT values indicating the adoption of an ARS behavior. We determined the effects of habitat variables on FPT using a methodological framework based on Linear Mixed Effect models and a randomization procedure intended to reduce spatial autocorrelation arising from consecutive circles whose areas tend to be overlapped along trajectories.
Results
Averaged model coefficients showed that woodpeckers spent less time in sites dominated by the Antarctic beech (Nothofagus antarctica) and partially covered by open habitats. Conversely, FPT increased in stands and home ranges where tree senescence was higher.
Conclusions
The adoption of the ARS behavior in Magellanic Woodpecker is explained by forest composition, avoidance of open habitats and tree senescence. Our results suggest the conservation of Magellanic Woodpeckers in heterogeneous landscapes involve retention of senescent trees and restoring open areas in order to reduce edge habitat.
LncRNA OGFRP1 acts as an oncogene in NSCLC via miR-4640-5p/eIF5A axis
Authors: Liu Xiaojing , Zhai Liping , Xiao Ke , Chen Wendan , Zhuang Xuewei 
Published: November 2020

Abstract
Background
Long noncoding RNAs (lncRNAs) OGFRP1 is up-regulated in endometrial cancer and cervical carcinoma, and OGFRP1 suppression inhibits the malignant behaviour of cancer cells. However, the role of OGFRP1 in non-small-cell lung cancer (NSCLC) have not been investigated. Here, we evaluated the expression pattern, biological function and potential mechanism of OGFRP1 in NSCLC.
Methods
We screened the siRNA (siOGFRP1) to down-regulate the expression of OGFRP1 in A549 and H1299 cells. The biological function of A549 and H1299 cells were examined by CCK8, wound healing and transwell assays. The molecular mechanism of OGFRP1 was further explored.
Results
siOGFRP1 significantly inhibited the cell proliferation, migration and invasion of A549 and H1299 cells. In addition, the expression of EMT-related and apoptosis-related proteins was changed by siOGFRP1 transfection. MiR-4640-5p could directly bind to the 3’ UTR region of eIF5A1. Moreover, OGFRP1 bound to miR-4640-5p through the same binding site, which facilitated the expression of eIF5A1. eIF5A1 overexpression rescued cell proliferation, migration and invasion inhibition induced by OGFRP1 down-regulation and miR-4640-5p up-regulation in A549 and H1299 cells.
Conclusions
Taken together, we demonstrated that down-regulation of OGFRP1 inhibited the progression of NSCLC through miR-4640-5p/eIF5A1 axis.