bluuebunny/crossref_metadata_2025_split huggingface dataset:
Successful percutaneous transvenous retrieval of intravascular fractured port catheter: a single center experience
Authors: Li Yahua , Chen Jianjian , Li Zhaonan , Lu Huibin , Ren Kewei , Ren Jianzhuang , Han Xinwei 
Published: May 2020

Abstract
Background
Fractured catheter as a foreign body in situ is a rare complication after port catheter placement. We report a single center’s experience on percutaneous transvenous retrieval of intravascular fractured port catheter and treatment techniques.

Methods
Patients undergoing percutaneous transvenous retrieval of intravascular fractured port catheter from Jan 2010 to Dec 2018 were retrospectively collected. A total of 10 patients (8 females and 2 males) were enrolled in this study. Procedures were performed within 1 day after diagnosis. Two methods of retrieval were considered, direct retrieval by gooseneck snare and guide wire as media to retrieve were used in the procedure.

Results
All the fractured catheters in 10 patients were successfully retrieval by 2 methods, direct retrieval by gooseneck snare(n = 6) and guide wire as media of retrieval(n = 4). The time interval between port catheter implantation and discovery of catheter fracture was 36.50 ± 42.99(ranged 1 to 146) days. The operation time was 24.10 ± 8.32(ranged 10 to 36) minutes. No immediate procedure related or 1 month follow-up complications occurred in all the 10 patients.

Conclusion
Percutaneous transvenous retrieval of intravascular fractured port catheter is a simple and safe procedure, which maybe recommended as the first choice for patients with fractured port catheter in situ.
Adequate timing and constant supervision are the keys for successful implementation of levothyroxine or levothyroxine/paracetamol absorption test
Authors: Lewandowski Krzysztof C. , Dąbrowska Katarzyna , Basińska-Lewandowska Magdalena , Bolanowski Marek , Ruchała Marek , Lewiński Andrzej 
Published: May 2020

Abstract
Background
Levothyroxine (LT4) pseudomalabsorption due to medication non-adherence results in significant costs for Health Service. High dose LT4 or LT4/paracetamol absorption test is used in such cases. Hence, establishment of an optimal test protocol and timing of sample collection is of utmost importance.

Case presentation
A 34-year old woman was admitted to our Department because of severe hypothyroidism [on admission thyrotropin (TSH) > 100 μIU/ml, free thyroxine (FT4) 0.13 ng/dl (ref. range 0.93–1.7)] despite apparently taking 1000 μg of LT4 a day. Autoimmune hypothyroidism had been diagnosed 4 years before during post-partum thyroiditis. Subsequently, it was not possible to control her hypothyroidism despite several admissions to two University Hospitals and despite vehement denial of compliance problems. There was no evidence of coeliac disease or other malabsorption problems, though gluten-free and lactose-free diet was empirically instigated without success. A combined paracetamol (1000 mg)/LT4 (1000 μg) absorption test was performed in one of these Hospitals. This showed good paracetamol absorption (from < 2 μg/ml to 14.11 μg/ml at 120 min), with inadequate LT4 absorption (FT4 increase from 5.95 pmol/l to 9.92 pmol/l at 0 and 120 min respectively). About 2 years prior to admission to our Department the patient was treated with escalating doses of levothyroxine [up to 3000 μg of T4 and 40 μg of triiodothyronine (T3) daily] without significant impact on TSH (still > 75 μIU/ml, and FT4 still below reference range).
After admission to our Department we performed a 2500 μg LT4 absorption test with controlled ingestion of crushed tablets, strict patient monitoring and sampling at 30 min intervals. We observed a quick and striking increase in FT4 from 0.13 to 0.46, 1.78, 3.05 and 3.81 ng/dl, at 0, 30, 60, 90 and 120 min, respectively. Her TSH concentration decreased to 13.77 μIU/ml within 4 days. When informed, that we had managed to “overcome” her absorption problems, she discharged herself against medical advice and declined psychiatric consultation.

Conclusions
Adequate patient supervision and frequent sampling (e.g. every 30 min for 210 min) is the key for successful implementation of LT4 absorption test. Paracetamol coadministration appears superfluous in such cases.
Gene modification strategies for next-generation CAR T cells against solid cancers
Authors: Tian Yonggui , Li Yilu , Shao Yupei , Zhang Yi 
Published: May 2020

AbstractImmunotherapies have become the backbone of cancer treatment. Among them, chimeric antigen receptor (CAR) T cells have demonstrated great success in the treatment of hematological malignancies. However, CAR T therapy against solid tumors is less effective. Antigen targeting; an immunosuppressive tumor microenvironment (TME); and the infiltration, proliferation, and persistence of CAR T cells are the predominant barriers preventing the extension of CAR T therapy to solid tumors. To circumvent these obstacles, the next-generation CAR T cells will require more potent antitumor properties, which can be achieved by gene-editing technology. In this review, we summarize innovative strategies to enhance CAR T cell function by improving target identification, persistence, trafficking, and overcoming the suppressive TME. The construction of multi-target CAR T cells improves antigen recognition and reduces immune escape. Enhancing CAR T cell proliferation and persistence can be achieved by optimizing costimulatory signals and overexpressing cytokines. CAR T cells equipped with chemokines or chemokine receptors help overcome their poor homing to tumor sites. Strategies like knocking out immune checkpoint molecules, incorporating dominant negative receptors, and chimeric switch receptors can favor the depletion or reversal of negative T cell regulators in the TME.
Severe diffuse alveolar hemorrhage related to autoimmune disease: a multicenter study
Authors: Mirouse Adrien , Parrot Antoine , Audigier Vincent , Demoule Alexandre , Mayaux Julien , Géri Guillaume , Mariotte Eric , Bréchot Nicolas , de Prost Nicolas , Vautier Mathieu , Neuville Mathilde , Bigé Naïke , de Montmollin Etienne , Cacoub Patrice , Resche-Rigon Matthieu , Cadranel Jacques , Saadoun David 
Published: May 2020

Abstract
Background
Diffuse alveolar hemorrhage (DAH) occurs during the course of autoimmune disease and may be life threatening. The objective was to assess characteristics and prognosis factors of DAH who required intensive care unit (ICU) admission in patients with autoimmune diseases.

Methods
French multicenter retrospective study including patients presenting DAH related to autoimmune diseases requiring ICU admission from 2000 to 2016.

Results
One hundred four patients (54% of men) with median age of 56 [32–68] years were included with 79 (76%) systemic vasculitis and 25 (24%) connective tissue disorders. All patients received steroids, and 72 (69%), 12 (11.5%), and 57 (55%) patients had cyclophosphamide, rituximab, and plasma exchanges, respectively. During ICU stay, 52 (50%), 36 (35%), and 55 (53%) patients required mechanical ventilation, vasopressor use, and renal replacement therapy, respectively. Factors associated with mechanical ventilation weaning were age (HR [95%CI] 0.97 [0.96–0.99] per 10 years, p < 0.0001), vasculitis-related DAH (0.52 [0.27–0.98], p = 0.04), and time from dyspnea onset to ICU admission (0.99 [0.99–1] per day, p = 0.03). ICU mortality was 15%. Factors associated with alive status at ICU discharge were chronic cardiac failure (HR [95%CI] 0.37 [0.15–0.94], p = 0.04), antiphospholipid syndrome-related DAH (3.17 [1.89–5.32], p < 0.0001), SAPS II (0.98 [0.97–0.99], p = 0.007), and oxygen flow at ICU admission (0.95 [0.91–0.99] per liter/min, p = 0.04).

Conclusion
DAH in autoimmune diseases is a life-threatening complication which requires mechanical ventilation in half of the cases admitted to ICU.
RNA structural dynamics regulate early embryogenesis through controlling transcriptome fate and function
Authors: Shi Boyang , Zhang Jinsong , Heng Jian , Gong Jing , Zhang Ting , Li Pan , Sun Bao-Fa , Yang Ying , Zhang Ning , Zhao Yong-Liang , Wang Hai-Lin , Liu Feng , Zhang Qiangfeng Cliff , Yang Yun-Gui 
Published: May 2020

Abstract
Background
Vertebrate early embryogenesis is initially directed by a set of maternal RNAs and proteins, yet the mechanisms controlling this program remain largely unknown. Recent transcriptome-wide studies on RNA structure have revealed its pervasive and crucial roles in RNA processing and functions, but whether and how RNA structure regulates the fate of the maternal transcriptome have yet to be determined.

Results
Here we establish the global map of four nucleotide-based mRNA structures by icSHAPE during zebrafish early embryogenesis. Strikingly, we observe that RNA structurally variable regions are enriched in the 3′ UTR and contain cis-regulatory elements important for maternal-to-zygotic transition (MZT). We find that the RNA-binding protein Elavl1a stabilizes maternal mRNAs by binding to the cis-elements. Conversely, RNA structure formation suppresses Elavl1a’s binding leading to the decay of its maternal targets.

Conclusions
Our study finds that RNA structurally variable regions are enriched in mRNA 3′ UTRs and contain cis-regulatory elements during zebrafish early embryogenesis. We reveal that Elavl1a regulates maternal RNA stability in an RNA structure-dependent fashion. Overall, our findings reveal a broad and fundamental role of RNA structure-based regulation in vertebrate early embryogenesis.
tappAS: a comprehensive computational framework for the analysis of the functional impact of differential splicing
Authors: de la Fuente Lorena , Arzalluz-Luque Ángeles , Tardáguila Manuel , del Risco Héctor , Martí Cristina , Tarazona Sonia , Salguero Pedro , Scott Raymond , Lerma Alberto , Alastrue-Agudo Ana , Bonilla Pablo , Newman Jeremy R. B. , Kosugi Shunichi , McIntyre Lauren M. , Moreno-Manzano Victoria , Conesa Ana 
Published: May 2020

AbstractRecent advances in long-read sequencing solve inaccuracies in alternative transcript identification of full-length transcripts in short-read RNA-Seq data, which encourages the development of methods for isoform-centered functional analysis. Here, we present tappAS, the first framework to enable a comprehensive Functional Iso-Transcriptomics (FIT) analysis, which is effective at revealing the functional impact of context-specific post-transcriptional regulation. tappAS uses isoform-resolved annotation of coding and non-coding functional domains, motifs, and sites, in combination with novel analysis methods to interrogate different aspects of the functional readout of transcript variants and isoform regulation. tappAS software and documentation are available athttps://app.tappas.org.
Anti-glaucoma potential of hesperidin in experimental glaucoma induced rats
Authors: Lu Baiyang , Wang Xue , Ren Zengjin , Jiang Haitao , Liu Bingqian 
Published: May 2020

AbstractGlaucoma is well-known clinical eye conditions that damage the optic nerve due to abnormal pressure conditions in eye. Hesperidin is well-known glycoside widely present in the citrus fruits, and its aglycone form is known as hesperetin. Hesperidin is major flavone found in orange fruits. Hypotensive effect of hesperidin in acute and chronic glaucoma rats, glutamate level in vitreous humour and glutathione (GSH) level in aqueous humour were determined following 25, 50 and 100 mg/kg of hesperidin treatment. Acetazolamide (5 mg/kg) was used as positive control. Hesperidin treatment significantly reduced the increased intraocular pressure (IOP) level in dextrose induced ocular hypertension than saline treated rats. The effect of hesperidin was comparable to the positive control acetazolamide. Similarly, hesperidin treatment significantly reduced the IOP level in prednisolone acetate induced ocular hypertension than saline treated rats. In the aqueous humour, hesperidin treatment increased the glutathione level 125%, 184.4% and 231.2% at 25, 50 and 100 mg/kg of hesperidin respectively. In the vitreous humour, hesperidin treatment reduced the glutamate level 9.9%, 13.2% and 25.3% at 25, 50 and 100 mg/kg of hesperidin respectively. Histopathological analysis of normal saline treated rats showed morphological alteration in ciliary bodies. However, rats treated with hesperidin showed the reduced level of morphological alteration in ciliary bodies. Taking all these data together, it is suggested that the hesperidin supplementation was effective against glaucoma in experimental rats.
Visual rhythms for qualitative evaluation of video stabilization
Authors: e Souza Marcos Roberto , Pedrini Helio 
Published: May 2020

AbstractRecent technological advances have enabled the development of compact and portable cameras for the generation of large volumes of video content. Several applications have benefited from such significant growth of multimedia data, such as telemedicine, surveillance and security, entertainment, teaching, and robotics. However, videos captured by amateurs are subject to unwanted motion or vibration while handling the camera. Video stabilization techniques aim to detect and remove glitches or instabilities caused during the acquisition process to enhance visual quality. In this work, we introduce and analyze a novel representation based on visual rhythms for qualitative evaluation of video stabilization methods. Experiments conducted on different video sequences are performed to demonstrate the effectiveness of the visual representation as qualitative measure for evaluating video stability. In addition, we present a proposal to calculate an objective metric extracted from the visual rhythms.
Still careless: findings from a cross-sectional study of young pedestrians’ risky road crossing behaviors
Authors: Hashemiparast Mina , Sharma Manoj , Asghari Jafarabadi Mohammad , Hosseini Zahra 
Published: May 2020

Abstract
Background
Pedestrian-vehicle collision is one of the most common traffic injuries worldwide. This study aimed to investigate the determinants of pedestrians’ road crossing beliefs and behaviors in potentially risky situations using the Theory of Planned Behavior among Iranian young adults.

Methods
This was a population-based study on a sample of 562 young adults aged 18 to 25 years living in Tehran, Iran. Data were collected by using a self-administered validated questionnaire including constructs of the theory of planned behavior and items of perceived risk and severity. The data were analyzed using independent t-test, analysis of covariance and multivariate analysis of variance.

Results
From all the respondents, 17.8% reported that they had previous experience of vehicle-collision. Among the participants, those who had previous experience of vehicle-collision reported less safety behaviors in crossing the road than those who had not experience an accident. It was found significant differences between participants with and without a history of vehicle-collision for perceived risk (mean difference, adjusted multivariate P-value: − 5.77, 0.027) and perceived severity (− 6.08, 0.003), attitude toward traffic regulations (− 6.34, 0.006), attitude toward behavior (− 7.56, 0.005), perceived behavioral control (− 5.20, 0.018), behavioral intention (− 5.35, 0.046) and road crossing behavior in potentially risky situations (− 5.37, 0.004).

Conclusions
Previous unpleasant experience of vehicle-collision is not the only determinant of self-protective behaviors in road- crossing which indicate the role of cognitive and motivational factors such as, subjective norms, attitudes towards risk, feelings of invulnerability in case of facing with vehicle collision.
What approaches exist to evaluate the effectiveness of UK-relevant natural flood management measures? A systematic map protocol
Authors: Connelly Angela , Snow Andrew , Carter Jeremy , Lauwerijssen Rachel 
Published: May 2020

Abstract
Background
Natural flood management (NFM) measures seek to protect, enhance, emulate, or restore the natural function of rivers as part of approaches to flood risk management (FRM). While there is agreement in both academic and practice/policy literature that NFM, in a general sense, should be part of a holistic FRM strategy to address current and future flood risk, the specifics of how to consistently implement NFM successfully in practice are less well known. This is particularly acute for practitioners in the UK given the nature of the UK’s biophysical and socio-political context. There is a recognition that existing reviews of NFM effectiveness in the UK tend to focus on the natural science basis and it is unclear how studies account for climate change. Further, reviews tend to focus only on UK studies. This systematic map aims to highlight the way in which existing NFM studies, from different disciplinary backgrounds and across Europe, evaluate effectiveness, and the extent to which they account for climate change. This knowledge can help to make recommendations for future areas of research where the multiple issues around understanding effectiveness can be synthesised, and where climate change is systematically taken into account.

Methods
This systematic map protocol addresses the following question: what approaches exist to evaluate the effectiveness of UK-relevant natural flood management measures? The protocol details the methodology that will be used to conduct a systematic map of the range of peer-reviewed journal papers, policy documents, guidance, and other forms of grey literature which currently exist on NFM to give an overview on the way in which the effectiveness of NFM is conceived. The methods detail the search strategy employed for gathering items across the peer-reviewed academic literature and grey literature. Additionally, the methods outline how the reviewers will approach article screening, and the eligibility criteria to include/exclude articles. The methods section also details the steps taken to ensure consistency across all reviewers, the data coding strategy, and methods for presenting the final systematic map. Together, the methods employed will help to identify current knowledge gaps, and will enable recommendations to be made for future research.
The advent of medical artificial intelligence: lessons from the Japanese approach
Authors: Ishii Euma , Ebner Daniel K. , Kimura Satoshi , Agha-Mir-Salim Louis , Uchimido Ryo , Celi Leo A. 
Published: May 2020

AbstractArtificial intelligence or AI has been heralded as the most transformative technology in healthcare, including critical care medicine. Globally, healthcare specialists and health ministries are being pressured to create and implement a roadmap to incorporate applications of AI into care delivery. To date, the majority of Japan’s approach to AI has been anchored in industry, and the challenges that have occurred therein offer important lessons for nations developing new AI strategies. Notably, the demand for an AI-literate workforce has outpaced training programs and knowledge. This is particularly observable within medicine, where clinicians may be unfamiliar with the technology. National policy and private sector involvement have shown promise in developing both workforce and AI applications in healthcare. In combination with Japan’s unique national healthcare system and aggregable healthcare and socioeconomic data, Japan has a rich opportunity to lead in the field of medical AI.
Egg investment strategies adopted by a desertic passerine, the Saxaul Sparrow (Passer ammodendri)
Authors: Bao Xinkang , Zhao Wei , Liu Fangqing , Li Jianliang , Ma Donghui 
Published: May 2020

AbstractBackgroundAs one of the reproductive strategies adopted by bird species, variation in investment in egg production and its influencing factors are important and well-studied subjects. Intraclutch changes in egg size associated with laying order may reflect a strategy of “brood survival” or “brood reduction” adopted by female birds in different situations.MethodsWe conducted field studies on the breeding parameters of the Saxaul Sparrow (Passer ammodendri) in Gansu Province, China from 2010 to 2017, to clarify the factors affecting the egg investment and reproductive performance of this passerine species.ResultsOur results revealed significant differences in clutch size, egg size and the fledging rate between the first and second brood of Saxaul Sparrows and suggested that this typical desert species allocates more breeding resources to the more favourable second brood period, leading to greater reproductive output. Female body size presented a positive relationship with egg size, and male body size presented positive relationships with clutch size and hatchability. The females that started their clutches later laid more eggs, and hatchability and the fledging rate also increased with a later laying date in the first brood period. With successive eggs laid within the 5-egg clutches (the most frequent clutch size), egg size increased for the first three eggs and then significantly decreased.ConclusionsOur results indicate that female Saxaul Sparrows increased egg investment because of good quality of paired males and good environmental conditions. The intraclutch variation of egg size suggests that this species inhabiting an arid environment adopts a “brood reduction” strategy.
Identifying best approaches for engaging patients and family members in health informatics initiatives: a case study of the Group Priority Sort technique
Authors: Lo Brian , Zhang Timothy , Leung Kevin , Mehta Rohan , Kuziemsky Craig , Booth Richard G. , Chyjek Anna , Rossetti Sarah Collins , McLean Drew , Borycki Elizabeth , McLay David , Noble Justin , Carter Shawn , Strudwick Gillian 
Published: May 2020

Abstract
Background
Patient engagement strategies in health service delivery have become more common in recent years. However, many healthcare organizations are challenged in identifying the best methods to engage patients in health information technology (IT) initiatives. Engaging with important stakeholders to identify effective opportunities can inform the development of a resource that addresses this issue and supports organizations in their endeavors. The purpose of this paper is to share our experience and lessons learned from applying a novel consensus-building technique in order to identify key elements for effective patient engagement in health IT initiatives. This will be done through a case study approach.

Methods
Patients, family members of patients, health professionals, researchers, students, vendor representatives and individuals who work in health IT roles in health organizations were engaged through a one-day symposium in Toronto, Canada in September, 2018. During the symposium, the Group Priority Sort technique was used to obtain structured feedback from symposium attendees in the context of small group discussions. Descriptive statistics and a content analysis were undertaken to analyze the data collected through the Group Priority Sort as well as participant feedback following the symposium.

Results
A total of 37 participants attended the symposium from a variety of settings and organizations. Using the Group Priority Sort technique, 30 topics were classified by priority to be included in a future resource. Participant feedback pertaining to the symposium and research methods was largely positive. Several areas of improvement, such as clarity of items, were identified from this case study.

Conclusions
The Group Priority Sort technique was an efficient method for obtaining valuable suggestions from a diverse group of stakeholders, including patients and family members. The specific priorities and feedback obtained from the symposium will be incorporated into a resource for healthcare organizations to aid them in engaging patients in health IT initiatives. Additionally, five important considerations were identified when conducting future work with the Group Priority Sort technique and are outlined in this paper.