bluuebunny/crossref_metadata_2025_split huggingface dataset:
Constraints on Earth System Functioning at the Paleocene‐Eocene Thermal Maximum From the Marine Silicon Cycle
Authors: Fontorbe Guillaume , Frings Patrick J. , De La Rocha Christina L. , Hendry Katharine R. , Conley Daniel J. 
Published: March 2020

AbstractThe Paleocene‐Eocene Thermal Maximum (PETM, ca. 56 Ma) is marked by a negative carbon isotope excursion (CIE) and increased global temperatures. The CIE is thought to result from the release of 13C‐depleted carbon, although the source(s) of carbon and triggers for its release, its rate of release, and the mechanisms by which the Earth system recovered are all debated. Many of the proposed mechanisms for the onset and recovery phases of the PETM make testable predictions about the marine silica cycle, making silicon isotope records a promising tool to address open questions about the PETM. We analyzed silicon isotope ratios (δ30Si) in radiolarian tests and sponge spicules from the Western North Atlantic (ODP Site 1051) across the PETM. Radiolarian δ30Si decreases by 0.6‰ from a background of 1‰ coeval with the CIE, while sponge δ30Si remains consistent at 0.2‰. Using a box model to test the Si cycle response to various scenarios, we find the data are best explained by a weak silicate weathering feedback, implying the recovery was mostly driven by nondiatom organic carbon burial, the other major long‐term carbon sink. We find no resolvable evidence for a volcanic trigger for carbon release, or for a change in regional oceanography. Better understanding of radiolarian Si isotope fractionation and more Si isotope records spanning the PETM are needed to confirm the global validity of these conclusions, but they highlight how the coupling between the silica and carbon cycles can be exploited to yield insight into the functioning of the Earth system.
Targeting the HGF/c-MET pathway in advanced pancreatic cancer: a key element of treatment that limits primary tumour growth and eliminates metastasis
Authors: Xu Zhihong , Pang Tony C. Y. , Liu Adele C. , Pothula Srinivasa P. , Mekapogu Alpha Raj , Perera Chamini J. , Murakami Takashi , Goldstein David , Pirola Romano C. , Wilson Jeremy S. , Apte Minoti V. 
Published: March 2020

Abstract
Background
Stromal–tumour interactions facilitate pancreatic cancer (PC) progression. The hepatocyte growth factor (HGF)/c-MET pathway is upregulated in PC and mediates the interaction between cancer cells and stromal pancreatic stellate cells (PSCs). This study assessed the effect of HGF/c-MET inhibition plus gemcitabine (G) on the progression of advanced PC.

Methods
Orthotopic PC was produced by implantation of luciferase-tagged human cancer cells + human PSCs into mouse pancreas. Tumours were allowed to develop without treatment for 4 weeks. Mice were then treated for 6 weeks with one of the following: IgG, G, HGF inhibitor (Hi), c-MET inhibitor (Ci), Hi + Ci, Hi + G, Ci + G, or Hi + Ci + G.

Results
Bioluminescence imaging showed similar tumour sizes in all mice at the initiation of treatments. Triple therapy (Hi + Ci + G): (1) completely eliminated metastasis; (2) significantly reduced tumour size as assessed by bioluminescence and at necropsy; (3) significantly reduced proliferating cancer cell density and stem cell marker DCLK1 expression in tumours. In vitro 3D culture studies supported our in vivo findings.

Conclusion
Even at an advanced disease stage, a two-pronged approach, targeting (a) HGF/c-MET with relevant inhibitors and (b) cancer cells with chemotherapy, completely eliminated metastasis and significantly decreased tumour growth, suggesting that this is a promising treatment approach for PC.
Young adulthood body mass index, adult weight gain and breast cancer risk: the PROCAS Study (United Kingdom)
Authors: Renehan Andrew G. , Pegington Mary , Harvie Michelle N. , Sperrin Matthew , Astley Susan M. , Brentnall Adam R. , Howell Anthony , Cuzick Jack , Gareth Evans D. 
Published: March 2020

Abstract
Background
We tested the hypothesis that body mass index (BMI) aged 20 years modifies the association of adult weight gain and breast cancer risk.

Methods
We recruited women (aged 47–73 years) into the PROCAS (Predicting Risk Of Cancer At Screening; Manchester, UK: 2009–2013) Study. In 47,042 women, we determined BMI at baseline and (by recall) at age 20 years, and derived weight changes. We estimated hazard ratios (HRs) and 95% confidence intervals (CIs) for new breast cancer using Cox models and explored relationships between BMI aged 20 years, subsequent weight changes and breast cancer risk.

Results
With median follow-up of 5.6 years, 1142 breast cancers (post-menopausal at entry: 829) occurred. Among post-menopausal women at entry, BMI aged 20 years was inversely associated [HR per SD: 0.87 (95% CI: 0.79–0.95)], while absolute weight gain was associated with breast cancer [HR per SD:1.23 (95% CI: 1.14–1.32)]. For post-menopausal women who had a recall BMI aged 20 years <23.4 kg/m2 (75th percentile), absolute weight gain was associated with breast cancer [HR per SD: 1.31 (95% CIs: 1.21–1.42)], but there were no associations for women with a recall BMI aged 20 years of >23.4 kg/m2 (Pinteraction values <0.05).

Conclusions
Adult weight gain increased post-menopausal breast cancer risk only among women who were <23.4 kg/m2 aged 20 years.
Ferritin in glioblastoma
Authors: Jaksch-Bogensperger Heidi , Spiegl-Kreinecker Sabine , Arosio Paolo , Eckl Peter , Golaszewski Stefan , Ebner Yvonne , Al-Schameri Rahman , Strasser Peter , Weis Serge , Bresgen Nikolaus 
Published: March 2020

AbstractElevated levels of serum ferritin (SF) are observed in several types of cancer; however, little is known on the association between ferritin and glioma, the most frequent type of human primary brain tumour. Here we report that GBM patients show significantly increased pre-surgical SF levels (i.e. ferritinaemia) within the SF reference range and a marked ferritin immunoreactivity of resected tumour tissue. Our findings account for an indirect association between ferritin synthesis in glioma-tissue and altered SF levels, which limits the clinical value of SF as a tumour marker in glioma. Importantly, we show for the first time that GBM-derived glioma cells release ferritin in vitro, which exerts an apoptosis-stimulating activity. Albeit the pathophysiologic context of apoptosis induction by a tumour-derived ferritin remains to be defined, our findings account for a distinct growth-regulatory role of these ferritin species in tumour biology.
HPV-mediated nuclear export of HP1γ drives cervical tumorigenesis by downregulation of p53
Authors: Yi Sang Ah , Lee Dong Hoon , Kim Go Woon , Ryu Hyun-Wook , Park Jong Woo , Lee Jaecheol , Han Jihoon , Park Jee Hun , Oh Hwamok , Lee Jieun , Choi Junjeong , Kim Hyun-Soo , Kang Hyeok Gu , Kim Da-Hyun , Chun Kyung-Hee , You Jueng Soo , Han Jeung-Whan , Kwon So Hee 
Published: March 2020

AbstractE6 oncoprotein derived from high-risk human papillomavirus (HPV) drives the development of cervical cancer through p53 degradation. Because cervical cancer therapies to inactivate HPV or E6 protein are not available, alternative strategies are required. Here, we show that HPV-mediated nuclear export of human heterochromatin protein 1γ (HP1γ) reduces the stability of p53 through UBE2L3-mediated p53 polyubiquitination during cervical cancer progression. In general, HP1 plays a key role in heterochromatin formation and transcription in the nucleus. However, our immunostaining data showed that the majority of HP1γ is localized in the cytoplasm in HPV-mediated cervical cancer. We found that HPV E6 protein drives unusual nuclear export of HP1γ through the interaction between the NES sequence of HP1γ and exportin-1. The mutation of the NES sequence in HP1γ led to nuclear retention of HP1γ and reduced cervical cancer cell growth and tumor generation. We further discovered that HP1γ directly suppresses the expression of UBE2L3 which drives E6-mediated proteasomal degradation of p53 in cervical cancer. Downregulation of UBE2L3 by overexpression of HP1γ suppressed UBE2L3-dependent p53 degradation-promoting apoptosis of cervical cancer cells. Our findings propose a useful strategy to overcome p53 degradation in cervical cancer through the blockage of nuclear export of HP1γ.
An expanded library of orthogonal split inteins enables modular multi-peptide assemblies
Authors: Pinto Filipe , Thornton Ella Lucille , Wang Baojun 
Published: March 2020

AbstractInteins are protein segments capable of joining adjacent residues via a peptide bond. In this process known as protein splicing, the intein itself is not present in the final sequence, thus achieving scarless peptide ligation. Here, we assess the splicing activity of 34 inteins (both uncharacterized and known) using a rapid split fluorescent reporter characterization platform, and establish a library of 15 mutually orthogonal split inteins for in vivo applications, 10 of which can be simultaneously used in vitro. We show that orthogonal split inteins can be coupled to multiple split transcription factors to implement complex logic circuits in living organisms, and that they can also be used for the in vitro seamless assembly of large repetitive proteins with biotechnological relevance. Our work demonstrates the versatility and vast potential of an expanded library of orthogonal split inteins for their use in the fields of synthetic biology and protein engineering.
Colonic microbiota is associated with inflammation and host epigenomic alterations in inflammatory bowel disease
Authors: Ryan F. J. , Ahern A. M. , Fitzgerald R. S. , Laserna-Mendieta E. J. , Power E. M. , Clooney A. G. , O’Donoghue K. W. , McMurdie P. J. , Iwai S. , Crits-Christoph A. , Sheehan D. , Moran C. , Flemer B. , Zomer A. L. , Fanning A. , O’Callaghan J. , Walton J. , Temko A. , Stack W. , Jackson L. , Joyce S. A. , Melgar S. , DeSantis T. Z. , Bell J. T. , Shanahan F. , Claesson M. J. 
Published: March 2020

AbstractStudies of inflammatory bowel disease (IBD) have been inconclusive in relating microbiota with distribution of inflammation. We report microbiota, host transcriptomics, epigenomics and genetics from matched inflamed and non-inflamed colonic mucosa [50 Crohn’s disease (CD); 80 ulcerative colitis (UC); 31 controls]. Changes in community-wide and within-patient microbiota are linked with inflammation, but we find no evidence for a distinct microbial diagnostic signature, probably due to heterogeneous host-microbe interactions, and show only marginal microbiota associations with habitual diet. Epithelial DNA methylation improves disease classification and is associated with both inflammation and microbiota composition. Microbiota sub-groups are driven by dominant Enterbacteriaceae and Bacteroides species, representative strains of which are pro-inflammatory in vitro, are also associated with immune-related epigenetic markers. In conclusion, inflamed and non-inflamed colonic segments in both CD and UC differ in microbiota composition and epigenetic profiles.
Larger-scale ocean-atmospheric patterns drive synergistic variability and world-wide volatility of wheat yields
Authors: Najafi Ehsan , Pal Indrani , Khanbilvardi Reza 
Published: March 2020

AbstractDiagnosing potential predictability of global crop yields in the near term is of utmost importance for ensuring food supply and preventing socio-economic consequences. Previous studies suggest that a substantial proportion of global wheat yield variability depends on local climate and larger-scale ocean-atmospheric patterns. The science is however at its infancy to address whether synergistic variability and volatility (major departure from the normal) of multi-national crop yields can be potentially predicted by larger-scale climate drivers. Here, using observed data on wheat yields for 85 producing countries and climate variability from 1961–2013, we diagnose that wheat yields vary synergistically across key producing nations and can also be concurrently volatile, as a function of shared larger-scale climate drivers. We use a statistical approach called robust Principal Component Analysis (rPCA), to decouple and quantify the leading modes (PC) of global wheat yield variability where the top four PCs explain nearly 33% of the total variance. Diagnostics of PC1 indicate previous year’s local Air Temperature variability being the primary influence and the tropical Pacific Ocean being the most dominating larger-scale climate stimulus. Results also demonstrate that world-wide yield volatility has become more common in the current most decades, associating with warmer northern Pacific and Atlantic oceans, leading mostly to global supply shortages. As the world warms and extreme weather events become more common, this diagnostic analysis provides convincing evidence that concurrent variability and world-wide volatility of wheat yields can potentially be predicted, which has major socio-economic and commercial importance at the global scale, underscoring the urgency of common options in managing climate risk.
The Limits of Effective Degrees of Freedom in UCA based Orbital Angular Momentum Multiplexed Communications
Authors: Li Zhipeng , Qu Fengzhong , Wei Yan , Yang Guowei , Xu Wen , Xu Jing 
Published: March 2020

AbstractOrbital angular momentum (OAM) multiplexing technique has recently emerged and generated widespread interests since the OAM was discovered as a property of electromagnetic wave and acoustic wave. It was widely acknowledged that OAM multiplexing can achieve very high effective degrees of freedom (EDOF) and improve the spectral efficiency in optical, radio and acoustic communications. However, in the field of free-space optical (FSO) communications, it was demonstrated that OAM multiplexing is not the optimal multiplexing technique and the spatial bandwidth product (SBP) limits the EDOF. Is there any EDOF limits of OAM multiplexing in radio and acoustic communications? Could OAM multiplexing be safely scaled to far field? Here, we discover that the azimuthal resolution of OAM mode generator in OAM multiplexing limits its EDOF. Furthermore, we also verify that the OAM multiplexing in radio and acoustic communication fails to enable a long distance transmission and high EDOF simultaneously incurred by the inherently imperfect OAM mode generator.
Marine virus predation by non-host organisms
Authors: Welsh Jennifer E. , Steenhuis Peter , de Moraes Karlos Ribeiro , van der Meer Jaap , Thieltges David W. , Brussaard Corina P. D. 
Published: March 2020

AbstractViruses are the most abundant biological entities in marine environments, however, despite its potential ecological implications, little is known about virus removal by ambient non-host organisms. Here, we examined the effects of a variety of non-host organisms on the removal of viruses. The marine algal virus PgV-07T (infective to Phaeocystis globosa) can be discriminated from bacteriophages using flow cytometry, facilitating its use as a representative model system. Of all the non-host organisms tested, anemones, polychaete larvae, sea squirts, crabs, cockles, oysters and sponges significantly reduced viral abundance. The latter four species reduced viral abundance the most, by 90, 43, 12 and 98% over 24 h, respectively. Breadcrumb sponges instantly removed viruses at high rates (176 mL h−1 g tissue dry wt−1) which continued over an extended period of time. The variety of non-host organisms capable of reducing viral abundance highlights that viral loss by ambient organisms is an overlooked avenue of viral ecology. Moreover, our finding that temperate sponges have the huge potential for constant and effective removal of viruses from the water column demonstrates that natural viral loss has, thus far, been underestimated.
De novo transcriptome assembly and analysis of Phragmites karka, an invasive halophyte, to study the mechanism of salinity stress tolerance
Authors: Nayak Soumya Shree , Pradhan Seema , Sahoo Dinabandhu , Parida Ajay 
Published: March 2020

AbstractWith the rapidly deteriorating environmental conditions, the development of stress tolerant plants has become a priority for sustaining agricultural productivity. Therefore, studying the process of stress tolerance in naturally tolerant species hold significant promise. Phragmites karka is an invasive plant species found abundantly in tropical and sub tropical regions, fresh water regions and brackish marshy areas, such as river banks and lake shores. The plant possesses the ability to adapt and survive under conditions of high salinity. We subjected P. karka seedlings to salt stress and carried out whole transcriptome profiling of leaf and root tissues. Assessing the global transcriptome changes under salt stress resulted in the identification of several genes that are differentially regulated under stress conditions in root and leaf tissue. A total of 161,403 unigenes were assembled and used as a reference for digital gene expression analysis. A number of key metabolic pathways were found to be over-represented. Digital gene expression analysis was validated using qRT-PCR. In addition, a number of different transcription factor families including WRKY, MYB, CCCH, NAC etc. were differentially expressed under salinity stress. Our data will facilitate further characterisation of genes involved in salinity stress tolerance in P. karka. The DEGs from our results are potential candidates for understanding and engineering abiotic stress tolerance in plants.
Effect of the deuterium on efficiency and type of adipogenic differentiation of human adipose-derived stem cells in vitro
Authors: Zlatska Alona V. , Vasyliev Roman G. , Gordiienko Inna M. , Rodnichenko Anzhela E. , Morozova Maria A. , Vulf Maria A. , Zubov Dmytro O. , Novikova Svitlana N. , Litvinova Larisa S. , Grebennikova Tatiana V. , Zlatskiy Igor A. , Syroeshkin Anton V. 
Published: March 2020

AbstractIn this study, we performed an adipogenic differentiation of human adipose-derived stem cells (ADSCs) in vitro with different deuterium content (natural, low and high) in the culture medium during differentiation process with parallel analysis of the gene expression, metabolic activity and cell viability/toxicity. After ADSCs differentiation into adipocytes we have done the analysis of differentiation process efficiency and determined a type of resulting adipocytes (by morphology, gene expression, UCP1 protein detection and adipokine production analysis). We have found that high (5 × 105 ppm) deuterium content significantly inhibit in vitro adipogenic differentiation of human ADSCs compared to the groups with natural (150 ppm) and low (30 ppm) deuterium content. Importantly, protocol of differentiation used in our study leads to white adipocytes development in groups with natural (control) and high deuterium content, whereas deuterium-depleted differentiation medium leads to brown-like (beige) adipocytes formation. We have also remarked the direct impact of deuterium on the cellular survival and metabolic activity. Interesting, in deuterium depleted-medium, the cells had normal survival rate and high metabolic activity, whereas the inhibitory effect of deuterated medium on ADSCs differentiation at least was partly associated with deuterium cytotoxicity and inhibitory effect on metabolic activity. The inhibitory effect of deuterium on metabolic activity and the subsequent decrease in the effectiveness of adipogenic differentiation is probably associated with mitochondrial dysfunction. Thus, deuterium could be considered as an element that affects the substance chirality. These findings may be the basis for the development of new approaches in the treatment of obesity, metabolic syndrome and diabetes through the regulation of adipose-derived stem cell differentiation and adipocyte functions.
Seltrapping in flat band lattices with nonlinear disorder
Authors: Rivas Danilo , Molina Mario I. 
Published: March 2020

AbstractWe study the transport properties of an initially localized excitation in several flat band lattices, in the presence of nonlinear (Kerr) disorder. In the weak nonlinearity regime, the dynamics is controlled by the degeneracy of the bands leading to a linear form of selftrapping. In the strong nonlinearity regime, the dynamics of the excitations depends strongly on the local environment around the initial excitation site that leads to a highly fluctuating selfrapping profile. For a binary nonlinear disorder, it is shown that the spreading of the flat band fundamental mode, is completely inhibited for a finite fraction of all cases. This fraction corresponds to the fraction of times the same value of (random) nonlinearity is assigned to all sites of the fundamental mode.
A New Fitting Method for Ambipolar Diffusion Length Extraction in Thin Film Structures Using Photoluminescence Measurement with Scanning Excitation
Authors: Chu Cheng-Hao , Mao Ming-Hua , Lin You-Ru , Lin Hao-Hsiung 
Published: March 2020

AbstractA new simple method is proposed to extract the ambipolar diffusion length for two-dimensional (2D) electronic transport in thin film structures using a scanning photoluminescence microscopy (SPLM) setup. No spatially-resolved photoluminescence detection methods are required. By measuring the excitation-position-dependent PL intensity across the edge of a semiconductor, ambipolar diffusion length can be extracted from the SPLM profile through a simple analytic fitting function. Numerical simulation was first used to verify the fitting method. Then the fitting method was applied to extract the ambipolar diffusion length from the measured SPLM profile of a GaAs thin film structure. Carrier lifetime was obtained in an accompanying time-resolved photoluminescence measurement under the same excitation condition, and thus the ambipolar diffusion coefficient can be determined simultaneously. The new fitting method provides a simple way to evaluate carrier transport properties in 2D electronic transport structures such as thin films or quantum wells.
Shear characteristics and stress-strain mathematical model of waste polyester textile reinforced clay
Authors: Lv Xiangfeng , Zhou Hongyuan 
Published: March 2020

AbstractClay reinforcement through appropriate applications of waste fiber or waste fiber fabric can generate huge economic and environmental benefits. In this study, clay was reinforced using waste polyester fiber filaments and waste polyester fabric blocks, respectively. Triaxial tests (σ1 > σ2 = σ3) were carried out to examine the influence of reinforcement method and the contents (0.0%, 0.5%, 1.0%, 1.5%) on the shear behavior of clay. After reinforcement, the deformation resistance and shear strength of the clay was improved. The optimal contents of fiber filament and fabric block were both 1.0%; as the fiber filament or fabric block content increased from 0.5% to 1.5%, the stiffness of the reinforced clay decreased, while the energy absorption capacity and the cohesive strength first increased and then decreased. Under the optimal content condition, the fiber filament showed better reinforcement than the fabric block. Under the train hardening condition, a hyperbolic model can be used to quantitatively describe the stress-strain relationship of the reinforced clay, and the model parameters can also reflect the strain hardening degree.