bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association of FTO methylation level with incident type 2 diabetes mellitus: a nested case–control study within the Rural Chinese Cohort Study
Authors: Huang Shengbing , Chen Qing , Zhang Dongdong , Cheng Cheng , Guo Chunmei , Li Quanman , Zhou Qionggui , Tian Gang , Qie Ranran , Han Minghui , Wu Xiaoyan , Zhao Yang , Yang Xingjin , Feng Yifei , Li Yang , Qin Pei , Zhao Yang , Zhang YanYan , Wu Yuying , Liu Dechen , Zhang Ming , Hu Dongsheng , Lu Jie 
Published: February 2020

Abstract
Objectives This study aimed to investigate the association of FTO methylation level with type 2 diabetes mellitus (T2DM). Methods We conducted a nested case – control study for DNA methylation of FTO in Chinese pople identified from the Rural Chinese Cohort Study with a 6-year follow-up. Controls were matched to the cases on a 1:1 basis by age, sex, ethnicity, marital status, and residence. Unconditional multivariate logistic regression models were used to calculate odds ratios (ORs) and 95% confidence intervals (CIs) for association with Tag-single nucleotide polymorphisms (SNPs) and cytosine guanine (CpG) locus. Haploview was used to analyze the association of possible haplotypes with T2DM. Generalized Multifactor Dimensionality Reduction was used to explore the potential interaction. Results We found a significant association for the CpG9 locus located in the promoter region of FTO with T2DM. With CpG9, the risk of T2DM was 1.84 (1.21–2.80) after adjusting for potential confounders. The Tag-SNPs (rs72803657, rs1558902, rs17817449, rs11076023) nor were possible haplotypes associated with T2DM in the strong linkage disequilibrium region. We found no significant interaction between obesity indexes, serum lipid levels and methylation levels. Conclusions Our study identified a CpG locus located in the promoter region of FTO that altered the T2DM risk independent of body mass index, but the variants of FTO were not significantly associated with T2DM. Our study might provide new insights into the pathways of FTO with T2DM and offer new opportunities for risk stratification and prevention of T2DM among rural Chinese population.
Waxy and Soluble Starch SynthaseII-3 Alleles Regulating Rice Resistant Starch Contents from Different Processing Status
Authors: you hui , Xu Liang , Zhang Ouling , Xiang Xunchao 
Published: February 2020

Abstract
BackgroundResistant Starch (RS) is a healthy dietary fiber that has functions of regulating diabetes, hypertension and obesity. Previous studies mainly focused on investigating RS in raw rice or cooked rice separately, which may receive different results. ResultsIn this study, ninety-nine lines from a recombinant inbred line (RIL) were selected to investigate the effects of starch synthesis-related genes on the RS content in different process status. RS content in rice will change by different processing ways. Waxy (Wx) played an important role in controlling RS content and Wxa could elevate RS content, and soluble starch synthaseII-3 (SSII-3) had an impact on RS2. Additionally, interaction of Wx and SSII-3 was responsible for variations of RS content in three sample types and RS2. Wx could affect RS in cooked rice and retrograded rice under the same SSII-3 allele. Moreover, the correlation analysis results indicated that RS was closely relative with many indexes of physicochemical properties. ConclusionsWx and SSII-3 could regulate RS content of rice, but SSII-3 especially affected RS2. The findings herein should provide useful information for molecular breeding of rice RS.
Long-term outcomes following vehicle trauma related acute kidney injury requiring renal replacement therapy: A nationwide population study
Authors: Chan Chieh-Kai , Prowle John R , Wu Vin-Cent 
Published: February 2020

Abstract
Background Acute kidney injury (AKI) is a frequent complication of traumatic injury; however, long-term outcomes such as mortality and end-stage kidney disease (ESKD) have been rarely reported in this important patient population. We compared the long-term outcome of traumatic and non-traumatic AKI requiring renal replacement therapy (AKI-RRT). Methods This nationwide cohort study used data from the Taiwan National Health Insurance Research Database. Vehicle-trauma patients developing AKI-RRT during hospitalization were identified, and matching non-traumatic AKI-RRT patients were identified between 2000 and 2010. The incidences of end-stage kidney disease (ESKD), 30-day, and long-term mortality were evaluated, and clinical and demographic associations with these outcomes were identified using Cox proportional hazards regression models. Results 546 traumatic AKI-RRT patients, median age 47.6 years (interquartile range: 29.0-64.3) and 76.4% male, were identified. Compared to non-traumatic AKI-RRT, traumatic AKI-RRT patients had longer length of stay in hospital [median (IQR):15 (5-34) days vs 6 (3-11) days; p < 0.001). After propensity matching with non-traumatic AKI-RRT cases with similar demographic and clinical characteristics. Traumatic AKI-RRT patients had lower rates of long-term mortality (adjusted hazard ratio (HR), 0.488; 95% CI, 0.405-0.588; p < 0.001), but similar rates of ESKD (HR, 1.075; 95% CI, 0.767–1.509; p = 0.674) and short-term risk of death (HR, 1.165; 95% CI, 0.920-1.476; p = 0.205) as non-traumatic AKI-RRT patients. Conclusions Despite severe injuries, traumatic AKI-RRT patients had better long-term survival than non-traumatic AKI-RRT patients, but a similar risk of ESKD. Our results provide a better understanding of long-term outcomes after traumatic AKI-RRT.
Loss of GLS2 function is essential for obtaining oncogenic functions and promotes the progression of clear cell renal cell carcinoma.
Authors: sun dantong , Tian Lu , Zhao Han , Yan Weihua , Wei Xiaojuan , Wo Yang , hou helei 
Published: February 2020

Abstract
Background The incidence of RCC has drastically increased in recent years. The large intratumor heterogeneity of RCC, especially ccRCC, usually leads to treatment failure. In addition, single biomarkers have a limited ability to predict prognosis. Therefore, we performed this study to select variables and provided a simple but efficient way to predict prognosis. Method Three studies from the GEO database were involved in the selection of DEGs. A total of 840 RCC patients and 524 ccRCC patients from the TCGA database were involved in the prognostic analyses. Nomograms based on the Cox regression model were used to select variables to predict the prognosis, and GSEA was used to demonstrate the potential pathways altered by gene expression. Result Our study suggested that DEGs existed between metastatic and primary tumor tissues. Loss of GLS2 function was related to poor prognosis in RCC and ccRCC. These results revealed that GLS2 expression combined with basic characteristics, including age and TNM stage, could efficiently predict prognosis. GLS2 serves as a tumor suppressor in ccRCC, and loss of GLS2 function endows cells with oncogenic functions and is related to advanced disease. According to the GSEA results, loss of GLS2 function may alter the cell cycle by activating the E2F pathway. Conclusion GLS2 is a tumor suppressor in RCC. Loss of GLS2 function in ccRCC predicts a poor prognosis via the E2F pathway. Nomograms based on DEGs and clinical features provide doctors with a simple but efficient way to predict prognosis. Further studies are needed to verify the pathway in our study.
Ovarian metastasis from non-gynecologic primary sites: a retrospective analysis of 177 cases and 13-year experience
Authors: Zhang Jingjing , Cao Dong-Yan , Yang Jia-Xin , Shen Keng 
Published: February 2020

Abstract
BackgroundMetastasis to the ovary is not rare. Generally, the most common non-gynecologic primary site of Ovarian metastasis is gastrointestinal tract. Differential diagnosis between primary malignant ovarian tumor and metastatic ovarian tumor is important, otherwise appropriate treatment cannot be determined. Furthermore, optimal treatment strategy for ovarian metastasis from non-gynecologic primary sites still are needed to explore. This study described the clinicopathologic characteristics of ovarian metastasis from non-gynecologic primary sites and identified the significance of surgical treatment for these patients. Methods One hundred and seventy-seven patients with ovarian metastasis from non-gynecologic primary sites admitted in Peking Union Medical College Hospital between May 2005 and May 2018 were retrospectively evaluated. Results The mean age was 48 years (range 18 - 83). Approximately 60% of patients were premenopausal women. The mostly two common non-gynecologic primary sites of ovarian metastasis were colorectum (68 cases) and stomach (61 cases). Besides the most common symptoms of abdominal distension (39.0%), abdominal pain (37.9%), ascites (27.7%), there were 18.1% of patients presenting with abnormal uterine bleeding. A half of patients who tested serum CA-125 preoperatively had an elevated CA-125 within the range of 35U/ml to 200U/ml. More than 70% of synchronous ovarian metastasis were misdiagnosed as primary ovarian cancer preoperatively. Of patients, 56.5% achieved optimal cytoreductive surgery (the diameter of largest residual lesion < 2 cm). The overall 5-year survival rate and median survival time were 10% and 20 months, respectively. The primary sites, optimal cytoreductive surgery, differentiation of tumor, and postoperative adjuvant treatment were prognostic indicators. Conclusions Colorectum and stomach are the most common non-gynecologic primary sites of ovarian metastasis. Synchronous ovarian metastasis is easy to be misdiagnosed as primary ovarian cancer. Optimal cytoreductive surgery and postoperative adjuvant treatment can be done to confer survival benefit in selected patients.
Effect of Yangyin Shuxin Decoction on Exercise Capacity in Patients with Heart Failure with Normal Ejection Fraction: Rationale and Design of a Randomized Controlled Trial
Authors: Zhao Zhiqiang , Wang Xianliang , Wang Shuai , Zhou Ruijuan , Su Quan , Liu Yu , Li Qing , Lin Shanshan , Liu Hua , Mao Jingyuan 
Published: February 2020

Abstract
Background The incidence of heart failure with normal ejection fraction (HFNEF) is increasing yearly, accounting for approximately half of all heart failure cases. Even after standardized treatment, the patient's prognosis is not good. Therefore, it is necessary to explore new treatment methods for HFNEF. Yangyin Shuxin Decoction, a traditional Chinese medicine prescription from our clinical experience in the treatment of HFNEF, has a potential cardioprotective effect. Preliminary clinical trials have shown that this prescription can improve the quality of life of HFNEF. This prompted us to use more objective indicators to further evaluate whether Yangyin Shuxin Decoction can improve the exercise capacity in HENEF patients. Methods This is a single-center parallel randomized controlled trial. The 64 patients who met the inclusion criteria were from the Cardiovascular Clinic. They will be randomly assigned to the treatment group (Yangying Shuxin Decoction combined with standard treatment) or the control group (standard treatment) according to the ratio of 1:1. The course of treatment will be 2 weeks. Both groups were interviewed at the following time points: of at enrollment (V1), and week 2 (V2), week 4 (V3), week 8 (V4), and week 12 (V5) after enrollment. The primary indicator is the peak oxygen consumption(Peak VO2) of the cardiopulmonary exercise test (CPET). Secondary indicators include CPET indicators such as anaerobic threshold oxygen consumption, carbon dioxide ventilation equivalent slope, echocardiographic indicators such as the ratio of mitral peak velocity of early filling to early diastolic mitral annular velocity(E/e′), left atrial volume index (LAVI), left ventricular mass index (LVMI), the peak velocity of tricuspid regurgitation (TR), B-type natriuretic peptide (BNP), New York Heart Association (NYHA) cardiac function grading, and so on. These indicators will be used to evaluate the effect of Yangyin Shuxin Decoction on exercise capacity in patients with HFNEF. Discussion At present, it is unclear whether the exercise capacity can be maintained after long-term use of Yangyin Shuxin Decoction. In this study, we will evaluate whether Yangyin Shuxin Decoction can improve the exercise capacity and quality of life of patients with HFNEF. This will provide an objective basis for the therapeutic effect of traditional Chinese medicine on HFNEF.
Protein delivery in intermittent and continuous enteral nutrition with a protein-rich formula in critically ill patients - A protocol for the prospective randomised controlled proof-of-concept Protein Bolus Nutrition (Pro BoNo) study
Authors: Reinhold Simona , Yeginsoy Désirée , Hollinger Alexa , Todorov Atanas , Tintignac Lionel , Sinnreich Michael , Kiss Caroline , Gebhard Caroline Eva , Kovács Balázs , Gysi Bianca , Imwinkelried Lara , Siegemund Martin 
Published: February 2020

Abstract
Background Critically ill patients rapidly develop muscle wasting resulting in sarcopenia, long-term disability and higher mortality. Bolus nutrition (30-60 min period), while having a similar incidence of aspiration as continuous feeding, seems to provide metabolic benefits through increased muscle protein synthesis due to higher leucine peaks. To date, clinical evidence on achievement of nutritional goals and influence of bolus nutrition on skeletal muscle metabolism in ICU patients is lacking. The aim of the Pro BoNo study ( Pro tein Bo lus N utriti o n) is to compare intermittent and continuous enteral feeding with a specific high-protein formula. We hypothesise that target quantity of protein is reached earlier (within 36 hours) by an intermittent feeding protocol with a favourable influence on muscle protein synthesis. Methods Pro BoNo is a prospective randomised controlled study aiming to compare the impact of intermittent and continuous enteral feeding on preventing muscle wasting in 60 critically ill patients recruited during the first 48 hours after ICU admission. The primary outcome measure is the time until the daily protein target (≥1.5 g protein/kg bodyweight/24h) is achieved. Secondary outcome measures include tolerance of enteral feeding and trajectory of glucose, urea and IGF-1. Ultrasound and muscle biopsy of the quadriceps will be performed. Discussion The Basel Pro BoNo study aims to collect innovative data on the effect of intermittent enteral feeding of critically ill patients on muscle wasting.
Effect of Gum Arabic (Acacia Senegal )on C-reactive protein level among Sickle Cell Anemia patients
Authors: Kaddam Lamis AbdelGadir , Kaddam Anas Suliman 
Published: February 2020

Abstract
Objectives: Inflammation is ongoing process among sickle cell anemia even during steady state. C reactive protein (CRP) is cardinal marker that utilized widely as inflammatory indicator. Gum Arabic (GA) is gummy exudates from Acacia Senegal tree. Fermentation by colonic bacteria increases serum butyrate concentrations, so considered as prebiotic agent. Gum Arabic (GA) has anti-inflammatory activity through butyrate. Earlier we proved that regular intake of GA increased fetal hemoglobin and anti-oxidant capacity most likely through raised level of butyrate, which would ameliorate symptoms of sickle cell anemia. Best of our knowledge this is the first study conducted to investigate GA intake on inflammatory markers among sickle patients.Results : This was a retrospective study conducted on stored samples from trial of Gum Arabic and sickle cell anemia. Quantitative CRP was measured by Mindray BS 200 before and after Gum Arabic consumption for 12 weeks. Daily intake of GA significantly decreased C reactive protein level (P.V=001) (95% CI, 0.943 -3.098). No correlation between CRP and age, fetal hemoglobin, hemolysis markers and white blood cells. Our findings revealed novel effect of GA as anti-inflammatory agent can be consumed as natural dietary supplement to modulate disease severity and downregulate inflammatory process.Trial registration: ClinicalTrials.gov Identifier: NCT02467257. Registered 3rd June 2015
Improved recording of work relatedness during patient consultations in occupational primary health care: A cluster randomized controlled trial using routine data
Authors: Atkins Salla , Reho Tiia , Talola Nina , Sumanen Markku , Viljamaa Mervi , Uitti Jukka 
Published: February 2020

Abstract
Background: Prolonging working careers is a key policy goal in ageing populations in Europe, but reaching this goal is complex. Occupational health services are in the best position to contribute towards prolonging working careers, through preventing illnesses that cause work disability and early pensions. However, impacting on the trajectory between illness and work disability requires continuity of care and follow-up, enabled through identifying at-risk clients. We aimed to determine whether a combined educational and electronic reminder system could improve the recording and follow-up of primary care visits made by patients at risk of work disability in occupational health care, and whether the system could impact on sickness absence rates.
Methods: This study is a pragmatic cluster randomized controlled trial using medical record data. Twenty two Pihlajalinna Työterveys units were randomized into an intervention group consisting of education and electronic reminders and a group receiving usual care through minimization methods. Client consultation data were extracted from routine Pihlajalinna Työterveys patient registers 2015 to 2017. In addition, process indicators were collected from the electronic system. Data were cleaned and analysed intention-to-treat using ANCOVA.
Results: There was no significant difference between intervention and control units in terms of sickness absences of different duration. Process indicators suggested that there was a change in physicians’ practice of recording clients’ risk of work disability and work relatedness of visits following the educational intervention.
Conclusion: Education with an electronic reminder can change physicians’ practice, but long-term follow-up is needed to determine whether this impacts on patients’ sickness absences.
Trial registration: ISRCTN45728263

Keywords: Occupational health; Randomized controlled trials; sickness absence; work-related illnesses
Penyuluhan Kesadaran Hukum Tentang Regulasi Alasan Perceraian dalam Hukum Perkawinan Islam bagi Keluarga Penerima Manfaat (KPM) Program Keluarga Harapan (PKH) di Kabupaten Grobogan
Authors: Rismana Daud 
Published: February 2020

<p>Found Beneficiary Families (KPM) The Family of Hope Program (PKH) whose marriage ties have been shaken, some even claim to have long been abandoned by their partners without news and certainty about the continuation of their marriage ties. So they can only surrender to accept the situation without knowing what to do. These problems are due to lack of knowledge and understanding of the reasons for divorce in Islamic marriage law. Then there is no opportunity yet to get legal awareness about the reasons for divorce in Islamic marriage law. As well as from both of these concerns, legal violations occur intentionally or unintentionally. The method used in this service is the lecture method, the discussion method, and the evaluation method. This training and outreach activity produced the first conclusion, this activity was very needed and was beneficial for PKH Beneficiary Families in Wirosari District. Secondly, There is an increase in the understanding and knowledge of the participants of this activity towards the regulations or legislation regarding the reasons for divorce in Islamic marriage law in Indonesia. The third is that not all PKH KPMs in Wirosari sub-district have the opportunity to participate in this activity because of limited funds.</p><p> </p><p>Ditemukan Keluarga Penerima Manfaat (KPM) Program Keluarga Harapan (PKH) yang ikatan perkawinannya telah goyah, bahkan ada yang mengaku telah lama ditinggalkan oleh pasangannya tanpa kabar dan kepastian mengenai kelanjutan ikatan perkawinan mereka. Sehingga mereka hanya bisa pasrah menerima keadaan tanpa tahu harus berbuat apa. Permasalahan-permasalahan tersebut dikarenakan kurangnya pengetahuan dan pemahaman mengenai alasan perceraian dalam hukum perkawinan Islam. Kemudian belum adanya kesempatan untuk mendapatkan penyuluhan kesadaran hukum tentang alasan perceraian dalam hukum perkawinan Islam. Serta dari kedua hal tersebut dikhawatirkan terjadi pelanggaran hukum baik disengaja maupun tidak disengaja. Adapun metode yang digunakan dalam kegiatan pengabdian ini adalah metode ceramah, metode diskusi, dan metode evaluasi. Kegiatan pelatihan dan penyuluhan ini menghasilkan kesimpulan yang pertama, kegiatan ini sangat dibutuhkan dan bermanfaat bagi Keluarga Penerima Manfaat PKH di Kecamatan Wirosari. Kedua, Terdapat peningkatan pemahaman dan pengetahuan para peserta kegiatan ini terhadap regulasi atau peraturan perundangan tentang alasan perceraian dalam hukum perkawinan islam di Indonesia. Yang ketiga adalah belum semua KPM PKH di Kecamatan Wirosari berkesempatan mengikuti kegiatan ini karena keterbatasan dana.</p>
Peningkatan Entrepreunership Melalui Inisiasi dan Pelatihan Pengemasan Wedang Pucuk Daun Jati (Tectona Grandis L.F)
Authors: Suwahono Suwahono , Mulyanti Sri 
Published: February 2020

<p class="ABSTRACT">The entrepreneurial spirit and value can be influenced through the initiation process, and business training. pharmacological analysis of teak shoots (Tectona Grandis l.f) had antibacterial, antioxidant, antifungal, anti-inflammatory, antipyretic, analgesic, anti-diuretic, and hypoglycemic activity. The use of teak leaves in the form of easy use should be disseminated and introduced through initiation and entrepreneurship training. The results of this studied indicate had a significant influence between initiation and training of teak leaf packaging with the entrepreneurial spirit and value. Entrepreneurship training can provide a good / positive contribution to the implementation of the business of students participating in the training because it helps in solving the obstacles that have been whacking business students.</p><p class="ABSTRACT"> </p><p class="ABSTRACT">Semangat dan nilai kewirausahaan dapat dipengaruhi melalui proses inisiasi, dan pelatihan bisnis. Analisis farmakologis tunas jati (Tectona Grandis l.f) memiliki aktivitas antibakteri, antioksidan, antijamur, antiinflamasi, antipiretik, analgesik, anti diuretik, dan hipoglikemik. Penggunaan daun jati dalam bentuk mudah digunakan harus disebarluaskan dan diperkenalkan melalui pelatihan inisiasi dan kewirausahaan. Hasil penelitian ini menunjukkan bahwa pelatihan memiliki pengaruh yang signifikan antara inisiasi dan pelatihan pengemasan daun jati dengan semangat dan nilai wirausaha. Pelatihan kewirausahaan dapat memberikan kontribusi yang baik / positif untuk pelaksanaan bisnis siswa yang berpartisipasi dalam pelatihan karena membantu dalam memecahkan hambatan yang telah memukul siswa bisnis.</p>