bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mechanical Ventilation Preserves Diaphragm Mitochondrial Function in a Rat Sepsis Model
Authors: Eyenga Pierre , Roussel Damien , Rey Benjamin , Ndille Patrice , Teulier Loic , Geuguen-Chaignon Virginie , Morel Jerome , Eyenga Florence , Romestaing Caroline , Sheu Shey-Shing 
Published: October 2020

Abstract
Background: To describe the effect of mechanical ventilation on diaphragm mitochondrial oxygen consumption, ATP production, reactive oxygen species (ROS) generation, and cytochrome-c oxidase activity and content, and their relationship to diaphragm strength in an experimental model of sepsis.Methods: A cecal ligation and puncture (CLP) protocol was performed in 12 rats while 12 controls underwent sham-operation. Half of the rats in each group were paralyzed and mechanically ventilated. We performed blood gas analysis and lactic acid assays 6 hours after surgery. Afterwards, we measured diaphragm strength and mitochondrial oxygen consumption, ATP and ROS generation, and cytochrome-c oxidase activity. We also measured malondialdehyde (MDA) content as an index of lipid peroxidation, and mRNA expression of the pro-inflammatory interleukin-1β (IL-1β) in diaphragms.Results: CLP rats showed severe hypotension, metabolic acidosis, and upregulation of diaphragm IL-1β mRNA expression. Compared to sham controls, spontaneously breathing CLP rats showed lower diaphragm force and increased susceptibility to fatigue, along with depressed mitochondrial oxygen consumption and ATP production and cytochrome-c oxidase activity. These rats also showed increased mitochondrial ROS generation and MDA content. Mechanical ventilation markedly restored mitochondrial oxygen consumption and ATP production in CLP rats; lowered mitochondrial ROS production by the complex 3; and preserved cytochrome-c oxidase activity.Conclusion: In an experimental model of sepsis, early initiation of mechanical ventilation restores diaphragm mitochondrial function.
Surgical Start Time Impact on Hospital Length of Stay for Elective Inpatient Procedures
Authors: Cifarelli Christopher , McMichael John P , Forman Alex G , Mihm Paul A , Cifarelli Daniel T , Lee Mark R , Marsh J. Wallis 
Published: October 2020

Abstract
Background: Hospital length of stay (LOS) remains an important metric in analysis of surgical services. Modifiable factors to reduce LOS are few in number and the ability to practically take action is limited. Surgical scheduling of elective cases remains an important task in optimizing workflow and may impact the post-surgical LOS.Methods: Retrospective data from a single tertiary care academic institution were analyzed for quality improvement of elective adult surgical cases performed from 2017 through 2019. Variables included primary procedure, age, diabetes status, ASA class, and surgical start time. Analysis of the median LOS following surgery was performed using Mann-Whitney tests and Cox hazards model. Matched-cohort analysis of mean total hospitalization costs was performed using the Student’s T-test. Results: 9258 patients were analyzed across five surgical service lines, of which 777 patients had surgical start time after 3PM. The median LOS for the after 3PM group was 1 day longer than the before 3PM start time cohort (3.0 vs 2.1, p<0.001). Service line analysis revealed increased LOS for Orthopedics and Neurosurgery (3.0 vs 1.9, p<0.001; 3.0 vs 2.0, p<0.05). Multivariate-analysis confirmed that start time before 3PM predicted shorter LOS (HR=1.214, 1.126-1.309; p<0.001). Case-matched cost analysis for frequently performed orthopedic and neurosurgical cases with an after 3PM start time failed to demonstrate a significant difference in total hospital charges. Conclusion: Optimization of surgical services scheduling to increase the proportion of elective surgical cases started before 3PM has the potential to decrease post-surgical LOS for adult patients undergoing Orthopedic or Neurosurgical procedures.
Treatment of Posttraumatic Stress Disorder Alleviates Tinnitus-related Distress Among Veterans: a Pilot Study
Authors: Moring John , Resick Patricia , Peterson Alan , Husain Fatima , Esqu Carlos , Young-McCaughan Stacey , Granato Elsa , Fox Peter 
Published: October 2020

Abstract
Background: Military service personnel are at increased risk for developing tinnitus due to heightened exposure to acoustic trauma. The auditory disorder is a leading service-connected disability among veterans and is highly comorbidly diagnosed with posttraumatic stress disorder (PTSD). The biopsychosocial model illustrates that chronic health conditions are exacerbated or maintained by psychiatric distress. Therefore, alleviation of such psychiatric distress can have beneficial impacts on health conditions, such as tinnitus. The aim of this study was to determine whether individuals with both disorders who receive evidence-based therapy for PTSD, will experience decreases in both PTSD and tinnitus-related distress.Methods: Veterans with comorbid bothersome tinnitus and PTSD received Cognitive Processing Therapy (CPT) and were assessed for PTSD, tinnitus-related distress, and depression at baseline and one month post-treatment follow-up.Results: At post-treatment follow-up, participants demonstrated significant decreases in PTSD symptoms compared to their baseline scores. Participants also demonstrated decreased tinnitus-related distress and depression, with high effect sizes.Discussion: This pilot study demonstrated the benefit of addressing psychiatric distress, such as PTSD, to simultaneously target bothersome tinnitus. Although not statistically significant due to the small sample size, large effect sizes indicate that tinnitus-related distress decreased as a function of receiving evidence-based therapy for PTSD. Future clinical trials should increase sample sizes and compare effects to control conditions.Trial RegistrationClinicalTrials.gov. NCT03702166. Registered October 10, 2018, https://clinicaltrials.gov/ct2/show/NCT03702166?cond=tinnitus&cntry=US&state=US%3ATX&city=San+Antonio&draw=1&rank=1
Younger Patients With Rheumatoid Arthritis Are as Dependent in Activities of Daily Living as Their Older Counterparts
Authors: Tufan Ayse Nur , Tufan Fatih , Oksuz Mustafa Ferhat , Ermurat Selime , Coskun Belkis Nihan , Pehlivan Yavuz , Dalkilic Ediz 
Published: October 2020

Abstract
Background: Rheumatoid arthritis is an important cause of disability and dependency in older adults. We aimed to assess dependency in activities of daily living in younger and older patients with rheumatoid arthritis and to seek factors underlying dependency in activities of daily living.Methods: In this cross-sectional study, we assessed dependency in basic and instrumental activities of daily living; disease activity; quality of life; depression; anxiety; and fatigue in the rheumatology clinic of a university hospital. Univariate analyses and a logistic regression analysis were performed to determine factors associated with dependency.Results: We enrolled 76 patients with a mean age of 57±11.1 (range 31-78). The rate of dependency in instrumental activities of daily living was high in younger (56.1%) and older (51.4%) patients. The rate of dependency in basic activities of daily living was 14.6% in younger and 14.3% in older patients. A higher depression score and female gender had significant associations with dependency in instrumental activities of daily living.Conclusion: The rate of dependency in basic and instrumental activities of daily living is high in younger and older patients with rheumatoid arthritis. Depression seems to be an independent and important factor underlying dependency. Further studies are needed to investigate the mechanisms of this association and test the effect of antidepressant treatment on dependency in rheumatoid arthritis.
Bioinformatics Analysis Combined with Experiments to Explore Neuroblastoma Prognostic Indicators Based on Immune-related Genes.
Authors: Shao FengLing , Wang Zhenni , Wang Shan 
Published: October 2020

Abstract
Background：Due to the extremely high mortality rate of children with high-risk Neuroblastoma (NB), there is an urgent need for new indicators to further classify children in the high-risk group for more precise treatment. The purpose of our research is to explore the immune-related genes in NB in the high-risk group, and to further identify and develop a prognostic nomogram based on immune IRG signatures. Methods：Through bioinformatics analysis to explore the abnormal expression of immune-related genes in the high-risk group. Cox regression and the least absolute shrinkage and selection operator (LASSO) analysis were conducted to identify the immune and overall survival (OS) related mRNA. The accuracy of the risk score is evaluated by Kaplan-Meier method and receiver operating characteristics (ROC) analysis, which is used to build a nomogram in combination with other clinical characteristics.. Quantitative real-time polymerase chain reaction (qRT-PCR) was conducted to detect the accuracy of our results. Results：A total of 127 common differentially expressed immune genes were found between the high-risk group and the non-high-risk group of the two data sets. Four immune-related genes (IRG) related to prognosis were identified and a risk score was established. Kaplan–Meier survival analysis and time-dependent ROC analysis showed that the 4-IRG risk score has satisfactory predictive potential and achieved consistency in the verification of external data sets. Subsequently, the risk score combined with clinical characteristics draws a nomogram. The reliability of the results was verified on 29 cases of NB tissues by qRT-PCR. Conclusions：Overall, we have developed a powerful multi-gene classifier that can effectively classify NB patients into low- and high-risk groups with poor prognosis, and draw a nomogram for children in the high-risk group. This feature can help select high-risk patients who need more aggressive adjuvant target therapy or immunotherapy.
A model for the kinetics of high-temperature reactions between polydisperse volcanic ash and SO2 gas
Authors: Wadsworth Fabian B. , Vasseur Jérémie , Casas Ana Silvia , Delmelle Pierre , Hess Kai-Uwe , Ayris Paul M. , Dingwell Donald B. 
Published: October 2020

AbstractRapid calcium diffusion occurs in rhyolitic volcanic ash particles exposed to hot SO2 atmospheres. Such chemical transport is important immediately following fragmentation, during proximal transport in eruption plumes and during percolative gas transport through a permeable volcanic edifice. Here we analyze published results of experiments designed to constrain the kinetics of this process. The experiments involve crushed rhyolitic glass particles tumbled in SO2-bearing atmospheres at a wide range of relevant temperatures. We find that the particle-gas reaction is fed by calcium diffusion from the bulk to the particle surfaces where calcium-sulfate crystals grow. The calcium flux is accommodated by local iron oxidation state changes. This process results in time-dependent concentrations of surface calcium that are leachable in aqueous solutions. Those leachate concentrations represent a proxy for the diffusive flux of Ca2+ out of the particle to form the surface deposits. We formulate a mathematical framework to convolve the starting particle size distributions with the solution to Fickian one-dimensional diffusion to find a weighted polydisperse result. Using this framework, we minimize for a temperature-dependent calcium diffusivity and compare our results with published calcium diffusivity data. We demonstrate that calcium diffusivity in rhyolite can be decomposed into two regimes: (1) a high-temperature regime in which the diffusivity is given by the Eyring equation and (2) a low-temperature regime more relevant to rhyolite volcanism and these gas-ash reactions. As a further test of our model, we compare the output against spatially resolved data for the calcium gradients in the experimental particles. Our analysis suggests that surface reaction rates are rapid compared with the diffusion of calcium from the particle to the surface, such that full diffusion models must be solved to predict the rhyolite-SO2 reaction. We conclude by suggesting how this framework could be used to make quantitative predictions of sulfur budgets and iron oxidation during rhyolitic eruptions.
Reply to “A comment on ‘An evolutionary system of mineralogy: Proposal for a classification of planetary materials based on natural kind clustering’”
Authors: Hazen Robert M. 
Published: October 2020

AbstractI welcome the “Comment” from Hatert et al. (2021) related to the proposal for an “Evolutionary system of mineralogy” (Hazen 2019) and thank them for their historically informed, conceptually nuanced, and consistently constructive contribution. They offer corrections related to two facets of my paper that seemed unfairly to criticize aspects of the International Mineralogical Association's Commission on New Minerals, Nomenclature and Classification (IMA-CNMNC) protocols for classifying minerals.First, they note an unfortunate inferred ambivalence with respect to the relationship between the IMA system and the new evolutionary system. If I was once ambivalent, that view has changed. Having spent the past two years in an ongoing effort to develop this new historical approach, I am struck every day by the power of the IMA-CNMNC system of species classification and nomenclature, which is fundamental and indispensable to the science of mineralogy. As Hatert et al. suggest, any new approach to organizing natural solids, including one focused on planetary evolution, must rest on the foundation provided by the IMA-CNMNC and its many volunteers who selflessly bring order to the mineral kingdom. In the best scenario, the evolutionary system may one day emerge as one of several useful approaches that complement and amplify but in no way replace this core IMA-CNMNC foundation, as clearly stated in the abstract of Hazen (2019).Second, Hatert et al. (2021) offer corrections regarding the IMA-CNMNC approach to classification, in particular a mischaracterization of the formal process to incorporate amorphous phases, poorly crystalline materials, and loosely defined “mineraloids.” I am grateful for the clarifications, as well as the implication that IMA protocols may facilitate the embrace of additional such phases in the future.Finally, I welcome the chance to explore further the emerging concept of “natural kinds” as applied to the mineral kingdom. Here, our thoughts differ. I suggest that minerals, considered in their information-rich, idiosyncratic, paragenetic contexts (in contrast to IMA-CNMNC species), have the potential to represent quintessential examples of “natural kinds.” Furthermore, when viewed in their evolutionary context, minerals offer an intriguing opportunity to expand the concept of “historical natural kinds” beyond its present limited and, at times, controversial use in biology, into the realm of the co-evolving geosphere and biosphere.
Semen Analysis and Morphological Study of Two Subsequent Generations of Dietary Protein Deficient Sprague Dawley Rats
Authors: Abey Nosarieme Omoregie , Ebuehi Osaretin Albert Taiwo 
Published: October 2020

Adequate supply of dietary protein in regular diet is an important element of a proper nutritional status as it functions in the regulation of metabolic profiles signaling the reproductive axis. Reports have highlighted the association of the amount of protein in the diet to the functioning of hypothalamic-pituitary-testis axis, however its intragenerational effects are yet to be adequately explored. The study aims to analyze the effect of protein deficient diet on parameters associated with male infertility from one generation to another. Female rats in four (4) groups (n=10) were fed different ration of protein in their diet (PD) as formulated: 21%PD, 10%PD, 5%PD and control diet (rat chow), through gestation and lactation to first and second generations, while the male offspring sustained on the maternal diet after weaning were used for analysis. Sperm morphology and motility were evaluated using a light microscope and a computer-assisted semen analysis. The percentage of motile spermatozoa, morphological characteristics and histology in the deficient groups (5%, 10%PD) appeared normal as compared to control in the first generation, showing an adaptive response while second generation was presented with significantly altered fertility parameters of the male offspring. It is suggested that in a deficiency status especially at the critical phase of development, certain deleterious effects which may be adaptive or phenotypic can serve to reduce the fertility efficiency in the male offspring at both F1 and F2- generation.
EFFECTS OF TYPE 2 DIABETES MELLITUS INDUCTION AND OF MODERATE PHYSICAL EXERCISE ON THE HEPATIC METABOLISM AND OXIDATIVE STRESS IN WISTAR RATS
Authors: Oliveira Denise Silva de , Santos Júlia Matzenbacher dos , Prado Kayorrany Gomes Galvão do , Amaral Andreia Vitor Couto , Benite-Ribeiro Sandra Aparecida 
Published: October 2020

AIMS: The aim of the study was to verify if the induction of Type 2 Diabetes Mellitus (T2DM) by the association of streptozotocin and high fat diet causes hepatic alterations and if physical exercise can improve these alterations. METHODS: Forty-six Wistar rats were divided into two groups. One group received a diet containing 60% of fat for 9 weeks and on the 14th day they were injected with a single dose of streptozotocin (25mg/kg). The other group, named control group, received a standard diet and a single dose of citrate buffer was given. Animals were them divided into two subgroups: one sedentary (S) and the other submitted to physical exercise (PE), totalizing four groups: C-S; C-PE; HF-S; HF-PE. After euthanasia blood and liver were collected. Plasma and hepatic concentrations of triacylglycerol and very low density lipoprotein; enzyme alanine transaminase, aspartate transaminase and arginase; urea, lactate and 8-isoprostane (an oxidative stress marker) were dosed. RESULTS: Capillary glucose was significantly higher in HF-S and HF-PE group animals compared to C-S and C-PE groups. There were no significant differences in alanine transaminase, arginase, oxidative stress and lactate concentration among the groups. Plasma urea concentration and hepatic aspartate transaminase concentration was lower in HF-S and HF-PE than in C-PE. Concerning liver triacylglycerol and very low density lipoproteins, both were higher in the HF-PE group when compared to the C-S. CONCLUSIONS: We conclude that diet was decisive for the aforementioned parameters alterations and that physical exercise protocol was not efficient to improve analyzed parameters.
Death Due to COVID-19 in an Infant with Combined Immunodeficiencies
Authors: Ahanchian Hamid , Moazzen Nasrin , Saeidinia Amin , Joghatayi Seyed H. , Khoshkhui Maryam , Aelami Mohammad H. , Haghi Nasrin S. M. , Rezaei Nima 
Published: October 2020

Background:
Severe Acute Respiratory Syndrome Coronavirus 2 has affected millions
of people, and especially in adult patients with underlying diseases lead to death. Meanwhile pediatric
patients with inherited defects of T cell should be prone to viral diseases.

Case Presentation:
Herein, we report an infant with severe combined immunodeficiencies, who
were affected and died because of COVID-19.

Conclusion:
Considering the importance of finding how immune system can affect the viral infection,
presentation of COVID-19 in immune deficient patients can be valuable.
The Role of Advanced Technologies Supplemented with Traditional Methods in Pharmacovigilance Sciences
Authors: Satwika Mandali V. , Sushma Dudala S. , Jaiswal Varun , Asha Syed , Pal Tarun 
Published: October 2020

Background:The immediate automatic systemic monitoring and reporting of adverse drug reactions, improving the efficacy is the utmost need of the medical informatics community. The venturing of advanced digital technologies into the health sector has opened new avenues for rapid monitoring. In recent years, data shared through social media, mobile apps, and other social websites has increased manifolds requiring data mining techniques.Objective:The objective of this report is to highlight the role of advanced technologies together with the traditional methods to proactively aid in the early detection of adverse drug reactions concerned with drug safety and pharmacovigilance.Methods:A thorough search was conducted on papers and patents regarding pharmacovigilance. All articles with respect to the relevant subject were explored and mined from public repositories such as Pubmed, Google Scholar, Springer, ScienceDirect (Elsevier), Web of Science, etc.Results:The European Union’s Innovative Medicines Initiative WEB-RADR project has emphasized the development of mobile applications and social media data for reporting adverse effects. Only relevant data has to be captured through the data mining algorithms (DMAs) as it plays an important role in timely prediction of risk with high accuracy using two popular approaches; the frequentist and Bayesian approach. Pharmacovigilance at the pre-marketing stage is useful for the prediction of adverse drug reactions in the early developmental stage of a drug. Later, post-marketing safety reports and clinical data reports are important to be monitored through electronic health records, prescription-event monitoring, spontaneous reporting databases, etc.Conclusion:The advanced technologies supplemented with traditional technologies are the need of the hour for evaluating a product’s risk profile and reducing risk in population especially with comorbid conditions and on concomitant medications.
Bifocal Disruption of the Patellar Tendon with Avulsion of the Tibial Tuberosity: A Case Report
Authors: Sekiguchi Takuya , Hagiwara Yoshihiro , Honda Masahito , Itoi Eiji 
Published: October 2020

Introduction:
Bifocal disruption of the knee extensor system is rare. Coincident bifocal proximal and distal disruptions of the patellar tendon are extremely rare in adults.

Case Report:
A 38-year-old man presented to our clinic with severe right knee pain after falling down the stairs and abruptly bending the right knee. Plain radiographs of this knee showed tibial avulsion and a high riding patella, suggesting underlying Osgood-Schlatter disease, which was also present to a milder degree in the left knee. Magnetic resonance imaging confirmed an avulsion of the tibial tuberosity and showed concomitant avulsion of the patellar tendon without bone marrow edema. Computed tomography showed that the fragment of the tibial tuberosity had a dull-edged margin, and cortical bones were partially exposed. During surgery, the patellar tendon was divided into superficial and deep layers. The superficial layer was peeled from an attachment at the patella, while the deep layer was from the tibia and contained the fragment of the tibial tuberosity. The detached side of the fragment and tibia were coated with dense, fibrous tissue. Surgical repair was performed, with excellent outcomes. Radiographic and intraoperative findings suggested Osgood-Schlatter disease, which might cause avulsion of the tibial tuberosity.

Conclusion:
This is the first case of the bifocal proximal and distal rupture of the patellar tendon with the avulsion of the tibial tuberosity. Bifocal disruption of the patellar tendon should be considered in patients presenting with avulsion of the tibial tuberosity and suspected Osgood-Schlatter disease.