bluuebunny/crossref_metadata_2025_split huggingface dataset:
A caregiver-reported global severity assessment in pediatric atopic eczema: the Comano score
Authors: Giovannini Mattia , Geat Davide , Barlocco Gabriele , Pertile Riccardo , Mori Francesca , Filippeschi Cesare , Novembre Elio , Cristofolini Mario , Baldo Ermanno 
Published: April 2020

AbstractAtopic eczema (AE) is the most common inflammatory skin disease in infancy and its prevalence is rising worldwide. It has a wide social impact on the affected children and their families’ lives. AE can have a chronic and heterogeneous course, with periods of remission and relapse of the clinical manifestations. For this reason, its severity assessment through standardized outcome measures becomes a fundamental guide for health professionals, who can manage AE following evidence-based medicine principles in their everyday clinical practice or in clinical trials.Several scoring systems have been recognized to assess the clinical manifestations of AE, both from the physician’s and the patient’s point of view. Despite the scoring systems standardized for adults, there are very few published options about the expression of a patient/caregiver-centered global severity assessment specifically for pediatric AE. For this reason, the aim of our study was to evaluate a new, quick, user-friendly and feasible caregiver-reported global severity assessment for pediatric AE. Based on a 0–10 numerical rating scale in pediatric AE, we named this scoring system the Comano score.We carried out a cross-sectional observational study enrolling a total of 867 patients aged from 1 to 16 years (males 49.5%, mean patient’s age 5.9 years, standard deviation ±3.6 years) with a previous doctor-confirmed diagnosis of AE, who underwent balneotherapy at Comano Thermal Center (Comano, Trentino, Italy). A strong correlation between Comano score and SCORing Atopic Dermatitis (SCORAD) was observed (r = 0.74, p < 0.0001).According to our results, the Comano score may be a promising new tool for the expression of a caregiver-reported global severity assessment in pediatric AE. However, further data are needed to confirm our preliminary findings before health professionals can use this scoring system in their everyday clinical practice to manage pediatric AE. Still, as a patient-focused measure, the Comano score may facilitate delivering person-centered care so as to define a measure for a clinical impact that can be meaningful to the subject, which is gaining importance in modern medicine.
Proactive versus standard support of labour in nulliparous women; study protocol for a randomized, controlled trial
Authors: Brenne Fehn Møyfrid , Dahlø Raija , Nielsen Renate , Laache Ingebjørg , Vanky Eszter 
Published: April 2020

Abstract
Background
Prolonged latent phase of labour often results in a traumatic birth experience. Prolonged labour is associated with more operative deliveries, haemorrhage, fetal asphyxia and poor birth experience. Women with prolonged labour in a former pregnancy more often demand caesarean section in the next, due to their negative birth experience. “Proactive support of labour” is an alternative method, developed to counteract prolonged labour. There are little research and no randomized controlled study that compare proactive to standard labour support.

Methods/Design
A prospective, non-blinded, randomized, single-centre, clinical trial where we compare proactive support to standard support of labour in a university hospital setting.
Inclusion criteria: latent phase of labour, non-pathologic pregnancy. Robson group 1, with painful contractions, and fully effaced cervix, with 1–3 cm dilatation. Exclusion criteria: induction of labour, breech presentation, twin pregnancy, multi-parity, conditions that require extended surveillance before and/or during labour.
Primary outcome: spontaneous, uncomplicated vaginal delivery. After inclusion, women randomized to proactive support of labour will stay at the hospital and have one-to-one midwife support. If no progression during the next 1–2 hours, amniotomy and/or oxytocin stimulation will be started. The control group will adhere to the standard procedures for labour support: expectance until established regular contractions and 4–5 cm cervical dilatation, and then one-to-one midwife support.

Discussion
The idea of proactive support of labour is to initiate early intervention when there are signs of slow progress in order to avoid protracted labour with exhaustion of the mother, the uterus and prolonged stress of the foetus. Proactive support of labour may represent a useful method to improve labour support in nulliparous women. However, evidence based on randomized controlled trials are needed in order to know whether proactive support of labour is comparable or superior to standard care. A randomized, controlled trial is described; challenges and possible clinical implications are discussed.

Trial registration
The Proactive Support of Labor Study (PAF) ClinicalTrials, NCT03056313. Registered on February 17, 2017.
Efficacy of digital cognitive behavioural therapy for symptoms of generalised anxiety disorder: a study protocol for a randomised controlled trial
Authors: Gu J. , Miller C. B. , Henry A. L. , Espie C. A. , Davis M. L. , Stott R. , Emsley R. , Smits J. A. J. , Craske M. , Saunders K. E. A. , Goodwin G. , Carl J. R. 
Published: April 2020

Abstract
Background
Generalised anxiety disorder (GAD) is a chronic and disabling condition with considerable personal and economic impact. Cognitive behavioural therapy (CBT) is a recommended psychological therapy for GAD; however, there are substantial barriers to accessing treatment. Digital CBT, in particular smartphone-delivered CBT, has the potential to improve accessibility and increase dissemination of CBT. Despite the emerging evidence of smartphone-based psychological interventions for reducing anxiety, effect size scores are typically smaller than in-person interventions, and there is a lack of research assessing the efficacy of smartphone-delivered digital interventions specifically for GAD.

Methods
In the DeLTA trial (DigitaL Therapy for Anxiety), we plan to conduct a parallel-group superiority randomised controlled trial examining the efficacy of a novel smartphone-based digital CBT intervention for GAD compared to a waitlist control. We aim to recruit 242 adults (aged 18 years or above) with moderate-to-severe symptoms of GAD. This trial will be conducted entirely online and will involve assessments at baseline (week 0; immediately preceding randomisation), mid-intervention (week 3), post-intervention (week 6; primary end point) and follow-up (week 10). The primary objective is to evaluate the efficacy of the intervention on GAD symptom severity compared to a waitlist control at post-intervention. Secondary objectives are to examine between-group effects on GAD at follow-up, and to examine the following secondary outcomes at both post-intervention and follow-up: 1) worry; 2) depressive symptoms; 3) wellbeing; 4) quality of life; and 5) sleep difficulty.

Discussion
This trial will report findings on the initial efficacy of a novel digital CBT intervention for GAD. Results have the potential to contribute towards the evidence base for digital CBT for GAD and increase the dissemination of CBT.

Trial registration
ISRCTN, ISRCTN12765810. Registered on 11 January 2019.
Medium-chain fatty acids and monoglycerides as feed additives for pig production: towards gut health improvement and feed pathogen mitigation
Authors: Jackman Joshua A. , Boyd R. Dean , Elrod Charles C. 
Published: April 2020

AbstractOngoing challenges in the swine industry, such as reduced access to antibiotics and virus outbreaks (e.g., porcine epidemic diarrhea virus, African swine fever virus), have prompted calls for innovative feed additives to support pig production. Medium-chain fatty acids (MCFAs) and monoglycerides have emerged as a potential option due to key molecular features and versatile functions, including inhibitory activity against viral and bacterial pathogens. In this review, we summarize recent studies examining the potential of MCFAs and monoglycerides as feed additives to improve pig gut health and to mitigate feed pathogens. The molecular properties and biological functions of MCFAs and monoglycerides are first introduced along with an overview of intervention needs at different stages of pig production. The latest progress in testing MCFAs and monoglycerides as feed additives in pig diets is then presented, and their effects on a wide range of production issues, such as growth performance, pathogenic infections, and gut health, are covered. The utilization of MCFAs and monoglycerides together with other feed additives such as organic acids and probiotics is also described, along with advances in molecular encapsulation and delivery strategies. Finally, we discuss how MCFAs and monoglycerides demonstrate potential for feed pathogen mitigation to curb disease transmission. Looking forward, we envision that MCFAs and monoglycerides may become an important class of feed additives in pig production for gut health improvement and feed pathogen mitigation.
A feasibility study to investigate the utility of a home-based exercise intervention during and after neo-adjuvant chemotherapy for oesophago-gastric cancer—the ChemoFit study protocol
Authors: Chmelo J. , Phillips A. W. , Greystoke A. , Charman S. J. , Avery L. , Hallsworth K. , Welford J. , Sinclair R. C. F. 
Published: April 2020

Abstract
Background
Treatment of locally advanced oesophago-gastric adenocarcinoma usually entails neo-adjuvant chemotherapy (NAC) and surgery. Surgery is associated with high morbidity and mortality. Cardiopulmonary reserve of patients having major surgery is related to postoperative outcomes. Complications are associated with poorer quality of life and may affect prognosis. Preventing complications may be beneficial to both of these and have cost implications. Prehabilitation may improve recovery from surgery by increasing a patients’ fitness before surgery. Designing a potentially cost and resource effective regimen which improves cardiopulmonary reserve may have a beneficial impact on patient outcomes after surgery.

Methods
The ChemoFit study is a non-randomised, single-arm and single-centre pilot study designed to investigate the feasibility of a home-based prehabilitation exercise intervention for patients receiving neoadjuvant treatment prior to oesophago-gastric surgery. Forty patients will be recruited at a single high-volume centre. The simple, home-based exercise intervention involves patients increasing their daily step-count during and after NAC and in the weeks leading up to surgical resection of the cancer. Additionally, quality of life assessments (QLQ-C30 and QLQ-OG25), oncological treatment delivery and participant perceptions of the study assessed by focus groups and questionnaires will be performed. The primary outcomes are to assess feasibility of the exercise intervention. The secondary outcomes will evaluate changes in cardiopulmonary reserve, sarcopenia and fat composition.

Discussion
It is anticipated that during an important teachable moment, the diagnosis and treatment of cancer, our patients will be open to the possibility of improving their fitness during chemotherapy and before major cancer surgery. It is possible that the negative impact of NAC on cardiopulmonary fitness could be prevented by implementing a home-based prehabilitation programme during and after NAC, prior to surgery for oesophago-gastric adenocarcinoma.

Trial registration
This study has been approved by the Health Research Authority (REC 18/WA/0427). Newcastle upon Tyne Hospitals NHS Foundation Trust (NUTH) will act as the study sponsor and the work is funded by a grant awarded by The Jon Moulton Charitable Foundation, supported by a research post funded by the Sir Bobby Robson Foundation. Trial registration: Clinicaltrials.gov, NCT04194463. Registered 11th December 2019—retrospectively registered, https://clinicaltrials.gov/ct2/show/NCT04194463.
In-vitro cytotoxic effects of Solanum macranthum fruit. Dunal extract with antioxidant potential
Authors: Kalebar Vishal U. , Hoskeri Joy H. , Hiremath Shivaprakash V. , Hiremath Murigendra B. 
Published: April 2020

Abstract
Background
The current study undertaken to evaluate antioxidant property of Solanum macranthum fruit methanol extract and its in-vitro cytotoxic effects on breast cancer MDA-MB-231 and mice embryo fibroblast (MEF-L929) cell line.

Methods
The total alkaloids, phenols and flavonoids content in the methanol extract of S.macranthum fruit were estimated. The antioxidant activity was evaluated by FRAP assay, H2O2 scavenging assay and DPPH assay. Anti-inflammatory protein denaturation inhibition assay was carried out using bovine serum albumin. Anticancer property of the extract was evaluated against breast cancer MDA-MB-231 by MTT assay and comparative study was carried out on normal cells using MEF-L929 cell line.

Results
Phytochemical estimation revealed that total alkaloid content was measurable high (13.6 g/100 g of extract), where as the total phenolics and flavonoids content was 115.5 mg/GAE and 142.6 mg/QE. Further, the antioxidant effect of methanol extract by H2O2 assay showed considerably higher scavenging activity with 99.6% at 400 μg/ml, where as by FRAP assay the relative reducing power was estimated to be 39.1% at 400 μg/ml and in case of DPPH assay it was found to be 47.9% at 400 μg/ml. EC50 value of anti-inflammatory assay was found to be 112.96 μg/ml. Anticancer studies showed that the IC50 value was found to be 373.77 μg /ml against MDA-MB-231 breast cancer cell line, where as the methanol extract showed negligible cytotoxic effect against normal cells (MEF -L929 cell line), indicating its cancer specific effect.

Conclusion
Based on the results obtained, methanol extract of Solanum macranthum fruit was found to possess significant antioxidant properties and also showed potential anticancer property against MDA-MB-231 breast cancer cell line with negligible cytotoxic effect on normal cells revealing its potential application as an anticancer agent.
Barcelona: municipalist policy entrepreneurship in a centralist refugee reception system
Authors: Garcés-Mascareñas Blanca , Gebhardt Dirk 
Published: April 2020

AbstractThis article is a case study of Barcelona’s policy entrepreneurship in innovating refugee reception. In a context of a highly centralised and increasingly dysfunctional Spanish refugee reception system, it traces how Barcelona, after declaring itself a city of refuge, aimed to change refugee reception into a more decentralised and bottom-up system with a major role of cities. Following Minstrom and Norman, the article reconstructs the city’s policy entrepreneurship and shows how the city 1) led by example in developing innovative local policies; 2) developed political arguments for change from a municipalist stance in coalition with other cities at national and EU level; and 3) worked its arguments into more technical discussions on the future of integration funding in the EU. On the basis of these developments, the article reaches two main conclusions. First, while the different registers of policy entrepreneurship cannot be directly connected to any concrete change of the multi-level setup of refugee reception, they do represent a significant step forward in cities’ self-organisation beyond ideological lines and a factor contributing to a stronger recognition of cities in refugee reception in Europe. Second, while the literature on multilevel governance has an in-built bias towards more coordinated and harmonious relationships across tiers, the case of Barcelona shows that more attention should be paid to conflict and change.
Is it time to review NICE guidelines on family therapy for anorexia in young people?
Authors: Woo Esther 
Published: April 2020

SUMMARYFamily therapy is recommended by the National Institute for Health and Care Excellence (NICE) for the management of anorexia nervosa in children and young people, but there is limited evidence to back this recommendation. The Cochrane Review under consideration evaluates the efficacy of different family therapy approaches compared with other treatments for anorexia nervosa, and this commentary puts the findings into clinical perspective.
Diagnosis and Management of Squamous Cell Carcinoma of Unknown Primary in the Head and Neck: ASCO Guideline
Authors: Maghami Ellie , Ismaila Nofisat , Alvarez Adriana , Chernock Rebecca , Duvvuri Umamaheswar , Geiger Jessica , Gross Neil , Haughey Bruce , Paul Doru , Rodriguez Cristina , Sher David , Stambuk Hilda E. , Waldron John , Witek Matt , Caudell James 
Published: April 2020

PURPOSE To provide evidence-based recommendations to practicing physicians and other health care providers on the diagnosis and management of squamous cell carcinoma of unknown primary in the head and neck (SCCUP). METHODS The American Society of Clinical Oncology convened an Expert Panel of medical oncology, surgery, radiation oncology, radiology, pathology, and advocacy experts to conduct a literature search, which included systematic reviews, meta-analyses, randomized controlled trials, and prospective and retrospective comparative observational studies published from 2008 through 2019. Outcomes of interest included survival, local and regional disease control, and quality of life. Expert Panel members used available evidence and informal consensus to develop evidence-based guideline recommendations. RESULTS The literature search identified 100 relevant studies to inform the evidence base for this guideline. Four main clinical questions were addressed, which included subquestions on preoperative evaluations, surgical diagnostic and therapeutic procedures, appropriate pathology techniques, and adjuvant therapy. RECOMMENDATIONS Evidence-based recommendations were developed to address preoperative evaluation for patients with a neck mass, surgical diagnostic and therapeutic procedures, appropriate treatment options in unilateral versus bilateral SCCUP. Additional information is available at www.asco.org/head-neck-cancer-guidelines .
Successful Implementation of a Perioperative Data Warehouse Using Another Hospital’s Published Specification From Epic’s Electronic Health Record System
Authors: Epstein Richard H. , Hofer Ira S. , Salari Valiollah , Gabel Eilon 
Published: April 2020

BACKGROUND:
Many hospitals have replaced their legacy anesthesia information management system with an enterprise-wide electronic health record system. Integrating the anesthesia data within the context of the global hospital information infrastructure has created substantive challenges for many organizations. A process to build a perioperative data warehouse from Epic was recently published from the University of California Los Angeles (UCLA), but the generalizability of that process is unknown. We describe the implementation of their process at the University of Miami (UM).

METHODS:
The UCLA process was tested at UM, and performance was evaluated following the configuration of a reporting server and transfer of the required Clarity tables to that server. Modifications required for the code to execute correctly in the UM environment were identified and implemented, including the addition of locally specified elements in the database.

RESULTS:
The UCLA code to build the base tables in the perioperative data warehouse executed correctly after minor modifications to match the local server and database architecture at UM. The 26 stored procedures in the UCLA process all ran correctly using the default settings provided and populated the base tables. After modification of the item lists to reflect the UM implementation of Epic (eg, medications, laboratory tests, physiologic monitors, and anesthesia machine parameters), the UCLA code ran correctly and populated the base tables. The data from those tables were used successfully to populate the existing perioperative data warehouse at UM, which housed data from the legacy anesthesia information management system of the institution. The time to pull data from Epic and populate the perioperative data warehouse was 197 ± 47 minutes (standard deviation [SD]) on weekdays and 260 ± 56 minutes (SD) on weekend days, measured over 100 consecutive days. The longer times on weekends reflect the simultaneous execution of database maintenance tasks on the reporting server. The UCLA extract process has been in production at UM for the past 18 months and has been invaluable for quality assurance, business process, and research activities.

CONCLUSIONS:
The data schema developed at UCLA proved to be a practical and scalable method to extract information from the Epic electronic health system database into the perioperative data warehouse in use at UM. Implementing the process developed at UCLA to build a comprehensive perioperative data warehouse from Epic is an extensible process that other hospitals seeking more efficient access to their electronic health record data should consider.
Cdc24 interacts with septins to create a positive feedback loop during bud site assembly in yeast
Authors: Chollet Julian , Dünkler Alexander , Bäuerle Anne , Vivero-Pol Laura , Mulaw Medhanie A. , Gronemeyer Thomas , Johnsson Nils 
Published: April 2020

ABSTRACT
Yeast cells select the position of their new bud at the beginning of each cell cycle. The recruitment of septins to this prospective bud site is one of the critical events in a complex assembly pathway that culminates in the outgrowth of a new daughter cell. During recruitment, septin rods follow the high concentration of Cdc42GTP that is generated by the focused localization of the Cdc42 guanine-nucleotide-exchange factor Cdc24. We show that, shortly before budding, Cdc24 not only activates Cdc42 but also transiently interacts with Cdc11, the septin subunit that caps both ends of the septin rods. Mutations in Cdc24 that reduce affinity to Cdc11 impair septin recruitment and decrease the stability of the polarity patch. The interaction between septins and Cdc24 thus reinforces bud assembly at sites where septin structures are formed. Once the septins polymerize to form the septin ring, Cdc24 is found at the cortex of the bud and directs further outgrowth from this position.
En bloc TGN recruitment of Aspergillus TRAPPII reveals TRAPP maturation as unlikely to drive RAB1-to-RAB11 transition
Authors: Pinar Mario , Peñalva Miguel A. 
Published: April 2020

ABSTRACT
Transport protein particle (TRAPP) complexes regulate membrane traffic. TRAPPII and TRAPPIII share a core hetero-heptamer, also denoted TRAPPI. In fungi TRAPPIII and TRAPPII mediate GDP exchange on RAB1 and RAB11, respectively, regulating traffic across the Golgi, with TRAPPIII also activating RAB1 in autophagosomes. Our finding that Aspergillus nidulans TRAPPII can be assembled by addition of a TRAPPII-specific subcomplex onto core TRAPP prompted us to investigate the possibility that TRAPPI and/or TRAPPIII already residing in the Golgi matures into TRAPPII to determine a RAB1-to-RAB11 conversion as Golgi cisternae progress from early Golgi to TGN identity. By time-resolved microscopy, we determine that the TRAPPII reporter Trs120 (the homolog of metazoan TRAPPC9) is recruited to existing trans-Golgi network (TGN) cisternae slightly before RAB11 arrives, and resides for ∼45 s on them before cisternae tear off into RAB11 secretory carriers. Notably, the core TRAPP reporter Bet3 (the homolog of metazoan TRAPPC3) was not detectable in early Golgi cisternae, being instead recruited to TGN cisternae simultaneously with Trs120, indicating en bloc recruitment of TRAPPII to the Golgi and arguing strongly against the TRAPP maturation model.