bluuebunny/crossref_metadata_2025_split huggingface dataset:
Numerical modeling of motion of displacement interface in eccentric annulus during primary cementing
Authors: Sun Jinfei , Li Zaoyuan , Luo Pingya , Huang Sheng 
Published: January 2020

AbstractCementing operations are of major importance to the long‐term zonal isolation and safety of oil and gas wells. As one of the well barriers, the cement sheath is key to the long‐term integrity of the wellbore. If the cement slurry does not completely fill the annulus between the casing and formation during the primary cementing displacement process, a potential leakage channel for subsurface fluids is created. In this study, the motion of the interface between the cement and other displaced fluids was examined during cementing displacement. By dividing an eccentric annulus into two 2D planes along the radial and azimuthal directions, a set of motion models of the interface were developed and solved numerically using a first‐order upwind finite‐difference method. The influences of modified density differenceF*, Bingham numbersB1andB2, plastic viscosity ratiom, eccentricitye*, and diffusion coefficientD*on the motion behavior of the displacement interface were determined. The results indicated that the interface of the eccentric annulus showed serious channeling and a physical mixing phenomenon. Some residual displaced fluid could remain at the casing‐cement and cement‐formation interfaces as a result of the improper design of the fluid parameters. KeepingF* > 0 andB2 < 5 beneficially removed residue from the displaced fluid on the wall, and increasingF*andB1(or decreasingB2,m,e*andD*) was conducive to the smooth motion of the displacement interface. This work showed that the interface motion model could predict the displacement quality and has great potential for improving the design of the primary cementing parameters.
Characteristics of fracture field in different stress zones during multi‐seam mining: Quantification based on theoretical analysis and BBM‐DEM accurate simulation method
Authors: Cheng Zhiheng , Ouyang Zhenhua , Zou Quanle , Lu Yun , Zhao Xidong , Li Meichen 
Published: January 2020

AbstractThe revelation of the distribution characteristics of stress and fractures in the floor strata is of great significance to improve the regional gas drainage efficiency and to eliminate or reduce the risk of coal and gas outburst in adjacent coal seams during multi‐seam mining. In this paper, the stress distribution in the floor under mining condition was derived, and the characteristics of stress zones in the floor were elaborated while mining the 22201 working face in Shaqu coal mine in Shanxi province, China. Besides, the propagation laws of fractures in different stress zones were theoretically analyzed, and the distribution law of fracture angles in the floor strata under different mining‐induced stresses was obtained. The research results demonstrate that the compression and transition zones show high vertical stress and less developed fractures and are dominated by fractures with a large angle (>60°), which is unfavorable for gas drainage. The expansion zone in the floor within 2‐40 m in the rear of the working face is characterized by high unloading degree of vertical stress and developed fractures. Moreover, fractures with a small angle (<60°) dominate the spatial distribution of the fractures, which is favorable for gas drainage. The research results can not only be a beneficial supplement to the theory of coal and gas co‐exploitation in multi‐seam mining but also provide theoretical support for optimizing the design parameters of cross‐measure boreholes under similar conditions.
Sampling and resolution characteristics in reduced order models of shallow water equations: Intrusive vs nonintrusive
Authors: Ahmed Shady E. , San Omer , Bistrian Diana A. , Navon Ionel M. 
Published: January 2020

SummaryWe investigate the sensitivity of reduced order models (ROMs) to training data spatial resolution as well as sampling rate. In particular, we consider proper orthogonal decomposition (POD), coupled with Galerkin projection (POD‐GP), as an intrusive ROM technique. For nonintrusive ROMs, we consider two frameworks. The first is using dynamic mode decomposition (DMD), and the second is based on artificial neural networks (ANNs). For ANN, we utilized a residual deep neural network, and for DMD we have studied two versions of DMD approaches; one with hard thresholding and the other with sorted bases selection. Also, we highlight the differences between mean subtracting the data (centering) and using the data without mean subtraction. We tested these ROMs using a system of 2D shallow water equations for four different numerical experiments, adopting combinations of sampling rates and spatial resolutions. For these cases, we found that the DMD basis obtained with hard thresholding is sensitive to sampling rate. The sorted DMD algorithm helps to mitigate this problem and yields more stabilized and converging solution. Furthermore, we demonstrate that both DMD approaches without mean subtraction provide significantly more accurate results than DMD with mean subtracting the data. On the other hand, POD is relatively insensitive to sampling rate and yields better representation of the flow field. Meanwhile, spatial resolution has little effect on both POD and DMD performances. Numerical results reveal that an ANN on POD subspace (POD‐ANN) performs remarkably better than POD‐GP and DMD in capturing system dynamics, even with a small number of modes.
MicroRNA‐based risk scoring system to identify early‐stage oral squamous cell carcinoma patients at high‐risk for cancer‐specific mortality
Authors: Yoon Angela J. , Wang Shuang , Kutler David I. , Carvajal Richard D. , Philipone Elizabeth , Wang Tian , Peters Scott M. , LaRoche Dominic , Hernandez Brenda Y. , McDowell Bradley D. , Stewart Claire R. , Momen‐Heravi Fatemeh , Santella Regina M. 
Published: January 2020

AbstractBackgroundFor early‐stage oral squamous cell carcinoma (OSCC), there is no existing risk‐stratification modality beyond conventional TNM staging system to identify patients at high risk for cancer‐specific mortality.MethodsA total of 568 early‐stage OSCC patients who had surgery only and also with available 5‐year clinical outcomes data were identified. Signature microRNAs (miRNAs) were discovered using deep sequencing analysis and validated by qRT‐PCR. The final 5‐plex prognostic marker panel was utilized to generate a cancer‐specific mortality risk score using the multivariate Cox regression analyses. The prognostic markers were validated in the internal and external validation cohorts.ResultsThe risk score from the 5‐plex marker panel consisting of miRNAs‐127‐3p, 4736, 655‐3p, TNM stage and histologic grading stratified patients into four risk categories. Compared to the low‐risk group, the high‐risk group had 23‐fold increased mortality risk (hazard ratio 23, 95% confidence interval 13‐42), with a median time‐to‐recurrence of 6 months and time‐to‐death of 11 months (vs >60 months for each among low‐risk patient; p < .001).ConclusionThe miRNA‐based 5‐plex marker panel driven mortality risk score formula provides clinically practical and reliable measures to assess the prognosis of patients assigned to an early‐stage OSCC.
In silico identification and characterization of antineoplastic asparaginase enzyme from endophytic bacteria
Authors: Zadeh Hosseingholi Elaheh , Neghabi Neda , Molavi Ghader , Gheibi Hayat Seyed Mohammad , Shahriarpour Hamed 
Published: January 2020

AbstractIt is generally accepted that L‐asparagine is an important amino acid required for the fast growth of cells. Cancerous cells receive this amino acid from extracellular sources. The depletion of L‐asparagine from its surrounding environments by asparaginase enzyme can be used as a therapeutic strategy in cancer patients. This therapeutic enzyme is produced commercially mainly from bacteria such as Escherichia coli and Erwinia chrysanthemi. The side effects of such drugs have persuaded scientists to find new enzyme sources. In this study, in silico approach was applied to investigate L‐asparaginase producing endophytic bacteria that produce more compatible enzymes within the body. Protein–protein basic local alignment search tool with E. coli and E. chrysanthemi asparaginase enzyme sequences against 262 endophytic bacteria were performed. The results with identity more than 35%, coverage more than 80%, and E‐value less than 10−4 were selected. Then, some of bioinformatics tools were used to characterize them. A total of nine sequences consisting of seven known and two hypothetical proteins were identified in six bacterial species. The results showed that some of the asparaginase enzymes produced by endophytic bacteria possess more suitable immunological indices compared with asparaginase enzymes of E. coli and E. chrysanthemi. Herbaspirillum rubrisubalbicans was predicted to produce a nonallergen and nonantigen asparaginase enzyme. The number of antigenic determinants was predicted to be lower in asparaginase enzymes produced by Bacillus amyloliquefaciens, H. rubrisubalbicans, and H. seropedicae. Moreover, the number of high‐scored B‐cell epitopes was lower in enzyme sequences related to the mentioned bacteria and Paenibacillus polymyxa. The number of discontinuous epitopes and the number of T‐cell epitopes were lower in B. amyloliquefaciens produced enzymes. Therefore, the therapeutic use of these enzymes is possible.
Nail matrix regenerative engineering: in vitro evaluation of poly(lactide‐co‐glycolide)/gelatin fibrous substrates
Authors: Montgomery Aundrya B. , McClinton Aneesah , Nair Lakshmi , Laurencin Cato T. 
Published: January 2020

AbstractAcute traumatic nail injury treatment repair procedures are commonly conducted in emergency departments and primary care offices. Current repair methods use nail splints that are inserted within the nail root to prevent the fusion of the proximal nail fold and the matrix tissue. Splints provide a protective barrier overlying the nail bed soft tissue during recovery periods, but uncertain prognoses (i.e., aesthetic and functional disadvantages) reveal a need for improved nail repair techniques. Nail splints are not specifically designed for nail organ restoration via biological mechanisms, thus, a clinical application that utilizes regenerative engineering techniques can prove useful in improving the nail injury prognoses. Using the coaxial electrospinning method, hybrid poly(lactide‐co‐glycolide) (PLGA) (85:15) and gelatin fibrous scaffolds (Hybrid1: PLGA shell, gelatin core and Hybrid2: gelatin shell, PLGA core) with average fiber diameters of 540 ± 118 and 2,215 ± 1,135 nm, respectively, were produced and successful encapsulation of core fibers was observed. Furthermore, nail stem cells expressing stem cell characteristic markers CD90, CD29, and Lgr6 showed preferred attachment to Hybrid2 scaffolds after 24 hr. Overall, an in vitro regenerative engineered nail matrix may aid to improve the cosmetic appearance and function of injured nail organs post‐traumatic injury.
Enhanced osteogenic differentiation of human mesenchymal stromal cells as response to periodical microstructured Ti6Al4V surfaces
Authors: Janßen Simon , Gach Stefan , Kant Sebastian , Aveic Sanja , Rütten Stephan , Olschok Simon , Reisgen Uwe , Fischer Horst 
Published: January 2020

AbstractTitanium‐based alloys, for example, Ti6Al4V, are frequently employed for load‐bearing orthopedic and dental implants. Growth of new bone tissue and therefore osseointegration can be promoted by the implant's microtopography, which can lead to improved long‐term stability of the implant. This study investigates the effect that an organized, periodical microstructure produced by an electron beam (EB) technique has on the viability, morphology, and osteogenic differentiation capacity of human mesenchymal stromal cells (hMSC) in vitro. The technique generates topographical features of 20 μm in height with varying distances of 80–240 μm. Applied alterations of the surface roughness and local alloy composition do not impair hMSC viability (>94%) or proliferation. A favorable growth of hMSC onto the structure peaks and well‐defined focal adhesions of the analyzed cells to the electron beam microstructured surfaces is verified. The morphological adaptation of hMSC to the underlying topography is detected using a three‐dimensional (3D) visualization. In addition to the morphological changes, an increase in the expression of osteogenic markers such as osteocalcin (up to 17‐fold) and osteoprotegerin (up to sixfold) is observed. Taken together, these results imply that the proposed periodical microstucturing method could potentially accelerate and enhance osseointegration of titanium‐based bone implants.
Therapeutic plasma exchange for exogenous insulin antibody syndrome in combined variable immunodeficiency: a case report
Authors: Robbins Daniel J. , Taylor Natalie E. , Narayanan Damodaran , Hess Aaron S. , Rose William N. 
Published: January 2020

AbstractA 32‐year‐old male with type I diabetes presented with profound hypoglycemia due to exogenous insulin antibody syndrome in the setting of newly‐diagnosed common variable immunodeficiency. Immunomodulatory therapy was not initially effective, but after the initiation of plasma exchange hypoglycemia resolved, and glucose lability improved.
Endothelin‐1 rs9296344 associates with the susceptibility of childhood primary nephrotic syndrome
Authors: Zhang Ruifeng , Yang Huandan , Zhu Bingbing , Yuan Tingting , Peng Qianqian , Lv Juan , Qiu Shan , Zhou Suqin , Li Yan , Zhong Zhaowen 
Published: January 2020

AbstractBackgroundRecently, the rs5370 single nucleotide polymorphisms (SNPs) of Endothelin‐1 (EDN1) showed association with the susceptibility of childhood primary nephrotic syndrome (CPNS). This study aims to investigate potential relationships between other EDN1 SNPs and CPNS.MethodsSeven SNPs (rs5370, rs10478723, rs1476046, rs1800541, rs2070698, rs2071942, and rs9296344) of the EDN1 gene were genotyped in 579 CPNS patients and 586 age‐matched healthy children. Then, we analyzed potential associations of the six SNPs with susceptibility of CPNS by using rs5370 as a conditional variant in a logistic regression model. SNP‐SNP interaction analysis was performed to investigate the joint effects of the seven SNPs in the pathogenesis of CPNS.ResultsIndependent with rs5370, only rs9296344 significantly associated (T vs C, odds ratio [OR] = 0.71, 95% confidence interval [CI] = 0.57‐0.88, P = .001) with the susceptibility of CPNS. Meanwhile, no joint effect among the analyzed seven SNPs was discovered in this study.ConclusionsThis study discovered that C allele of rs9296344 on EDN1 is a novel independent risk factor for CPNS.
Self‐compassion, meaning in life, and experiential avoidance explain the relationship between meditation and positive mental health outcomes
Authors: Yela José Ramón , Crego Antonio , Gómez‐Martínez María Ángeles , Jiménez Laura 
Published: January 2020

AbstractObjectiveDespite consistent evidence for the beneficial effects of meditation on mental health, little is known about the mechanisms that make mindfulness meditation effective.MethodThe levels of mental health, self‐compassion, presence of meaning in life, and experiential avoidance of meditators (n = 414) and nonmeditators (n = 414) were measured and compared. Bootstrap‐based structural equation modeling (SEM) modeling analyses were used to test multiple‐step multiple‐mediator models.ResultsMeditation was positively associated with mental health, although the regularity of practice was an influential element to be considered. Significant indirect effects of meditation on mental health through self‐compassion, meaning in life, and experiential avoidance were found. SEM models were able to account for 58% of the variance in mental health scores.ConclusionsSelf‐compassion, presence of meaning in life, and reduced experiential avoidance may be active components of healthy meditation practices. Identifying the mechanisms involved in effective meditation practices has relevant implications for well‐being and mental health‐promoting interventions.
ERO1α promotes testosterone secretion in hCG‐stimulated mouse Leydig cells via activation of the PI3K/AKT/mTOR signaling pathway
Authors: Chen Fenglei , Wang Yujing , Liu Qinguang , Hu Jiahui , Jin Jiaqi , Ma Zhiyu , Zhang Jinlong 
Published: January 2020

AbstractER oxidoreduclin 1α (ERO1α) is an oxidase, participating in formation of secretory and membrane proteins. However, the other physiological functions ERO1α is not well known. We found that ERO1α is high in the Leydig cells of the testis. Therefore, the purposes of the current study are to explore the role of ERO1α and the possible mechanisms in regulating cell proliferation, apoptosis, and testosterone secretion of Leydig cells. ERO1α was mainly localized in Leydig cells in the adult mice testes by immunofluorescence staining. Western blot analysis showed that ERO1α was higher in Leydig cells than that in the seminiferous tubules. The effect of ERO1α on cell proliferation, apoptosis, and testosterone secretion was detected by transducing ERO1α overexpression and knockdown lentiviruses into cultured primary Leydig cells (PLCs) together with hCG exposure. Flow cytometry analysis showed that ERO1α promoted cell proliferation by increasing cell distribution at the S phase and decreasing that at the G0/G1 phase. Western bolt analysis showed that ERO1α increased CDK2 and CDK6 expression. Cell apoptosis determination found that ERO1α inhibited PLC apoptosis. Western bolt analysis showed that ERO1α increased the ratio of BCL‐2/BAX, and decreased BAD and Caspase‐3 expression. Enzyme‐linked immunosorbent assay analysis demonstrated that ERO1α enhanced testosterone secretion. Western bolt analysis found that ERO1α increased StAR, 3β‐HSD, and CYP17A1 expression. Furthermore, ERO1α could activate the PI3K/AKT/mTOR signaling pathway. In summary, these results suggest that ERO1α might play proliferation promotion and antiapoptotic roles and enhance testosterone secretion in PLC, at least partly, via activation of the PI3K/AKT/mTOR signaling pathway.
Activation of CB2R with AM1241 ameliorates neurodegeneration via the Xist/miR‐133b‐3p/Pitx3 axis
Authors: He Xiaolie , Yang Li , Huang Ruiqi , Lin Lijuan , Shen Yijue , Cheng Liming , Jin Lingjing , Wang Shilong , Zhu Rongrong 
Published: January 2020

AbstractActivation of cannabinoid receptor type II (CB2R) by AM1241 has been demonstrated to protect dopaminergic neurons in Parkinson's disease (PD) animals. However, the specific mechanisms of the action of the CB2R agonist AM1241 for PD treatment have not been characterized. Wild‐type (WT), CB1R knockout (CB1‐KO), and CB2R knockout (CB2‐KO) mice were exposed to 1‐methyl‐4‐phenyl‐1,2,3,6‐tetrahydropyridine (MPTP) for 1 week to obtain a PD mouse model. The therapeutic effects of AM1241 were evaluated in each group. Behavioral tests, analysis of neurotransmitters, and immunofluorescence results demonstrated that AM1241 ameliorated PD in WT animals and CB1‐KO animals. However, AM1241 did not ameliorate PD symptoms in CB2‐KO mice. RNA‐seq analysis identified the lncRNA Xist as an important regulator of the protective actions of AM1241. Specifically, AM1241 allowed WT and CB1‐KO animals treated with MPTP to maintain normal expression of Xist, which affected the expression of miR‐133b‐3p and Pitx3. In vitro, overexpression of Xist or AM1241 protected neuronal cells from death induced by 6‐hydroxydopamine and increased Pitx3 expression. The CB2 receptor agonist AM1241 alleviated PD via regulation of the Xist/miR‐133b‐3p/Pitx3 axis, and revealed a new approach for PD treatment.
miR‐26a attenuates cardiac apoptosis and fibrosis by targeting ataxia–telangiectasia mutated in myocardial infarction
Authors: Chiang Ming‐Hsien , Liang Chan‐Jung , Lin Lung‐Chun , Yang Yi‐Fan , Huang Ching‐Chang , Chen Ying‐Hsien , Kao Hsien‐Li , Chen Yu‐Chen , Ke Shin‐Rong , Lee Chiang‐Wen , Lin Mao‐Shin , Chen Yuh‐Lien 
Published: January 2020

AbstractApoptosis and fibrosis play a vital role in myocardial infarction (MI) induced tissue injury. Although microRNAs have been the focus of many studies on cardiac apoptosis and fibrosis in MI, the detailed effects of miR‐26a is needed to further understood. The present study demonstrated that miR‐26a was downregulated in ST‐elevation MI (STEMI) patients and oxygen‐glucose deprivation (OGD)‐treated H9c2 cells. Downregulation of miR‐26a was closely correlated with the increased expression of creatine kinase, creatine kinase‐MB and troponin I in STEMI patients. Further analysis identified that ataxia–telangiectasia mutated (ATM) was a target gene for miR‐26a based on a bioinformatics analysis. miR‐26a overexpression effectively reduced ATM expression, apoptosis, and apoptosis‐related proteins in OGD‐treated H9c2 cells. In a mouse model of MI, the expression of miR‐26a was significantly decreased in the infarct zone of the heart, whereas apoptosis and ATM expression were increased. miR‐26a overexpression effectively reduced ATM expression and cardiac apoptosis at Day 1 after MI. Furthermore, we demonstrated that overexpression of miR‐26a improved cardiac function and reduced cardiac fibrosis by the reduced expression of collagen type I and connective tissue growth factor (CTGF) in mice at Day 14 after MI. Overexpression of miR‐26a or ATM knockdown decreased collagen I and CTGF expression in cultured OGD‐treated cardiomyocytes. Taken together, these data demonstrate a prominent role for miR‐26a in linking ATM expression to ischemia‐induced apoptosis and fibrosis, key features of MI progression. miR‐26a reduced MI development by affecting ATM expression and could be targeted in the treatment of MI.
Spontaneous external jugular vein pseudoaneurysm treated by thrombin injection: Case report and literature review
Authors: Dogan Sebahat N. , Kazcı Zeynep N. , Kavuncu Selen B. , Bayrak Aylin H. 
Published: January 2020

AbstractAlthough external jugular vein (EJV) aneurysms are infrequent, regardless of etiology, spontaneous pseudoaneurysms (PAs) are extremely rare and generally require surgery. We describe a case of spontaneous PA of the EJV, which was successfully treated by percutaneous thrombin injection.
Morphine consumption during pregnancy exacerbates neonatal hypoxia‐ischemia injury in rats
Authors: Bornavard Morad , Fanaei Hamed , Mirshekar Mohammad Ali , Farajian Mashhadi Farzaneh , Atashpanjeh Alireza 
Published: January 2020

AbstractObjectiveHypoxia‐Ischemia (HI) is the most common cause of death and disability in human infants. The use of opiate in pregnant women affects their children. The aim of this study was to evaluate the effect of morphine consumption during pregnancy and lactation on vulnerability to neonatal HI in rats.Materials and methodsFemale Wistar rats were randomly assigned into two groups: Group 1‐Rats that did not receive any treatment during pregnancy and lactation and Group 2‐Rats that received morphine during pregnancy and lactation. After delivery, male offspring were divided into four groups including: (a) SHAM, (b) SHAM/Morphine (SHAM/MO), (c) HI, (d) HI/Morphine (HI/MO). Seven days after HI induction, neurobehavioral tests were performed, and then, brain tissue was taken from the skull to measure cerebral edema, infarct volume, inflammatory factors, oxidative stress, and brain‐derived neurotrophic factor (BDNF).ResultsTotal antioxidant capacity (TAC) and BDNF levels in the HI/MO group were significantly lower than HI and SHAM groups. TNF‐α, C‐reactive protein and total oxidant capacity levels in the HI/MO group were significantly higher than HI and SHAM groups. Cerebral edema and infarct volume in the HI/MO group were significantly higher than the HI group.ConclusionBased on the results, morphine consumption during pregnancy and lactation enhanced the deleterious effects of HI injury in pups.