bluuebunny/crossref_metadata_2025_split huggingface dataset:
Enterococcus durans with mosquito larvicidal toxicity against Culex quinquefasciatus, elucidated using a Proteomic and Metabolomic approach
Authors: Colvin Domnic , Dhuri Vishnu , Verma Hirday , Lokhande Rama , Kale Avinash 
Published: March 2020

AbstractVarious bacteria from the Bacillus species have been used as pesticides against mosquito larvae for more than a decade. The prolonged use of these bacterial species by little alteration within their genome, using various permutations and combinations of mosquito-cidal toxins, has proven unsuccessful in controlling the mosquito population. In our current study we report Enterococcus sp. to be exhibiting similar kind of mosquito-cidal toxins alike those which are present in the mainly used Bacillus strains. Three Enterococcus species were isolated on a rich media selective for gram- positive bacteria from the mid-gut of dead mosquito larvae which were collected from the wild locations within and around the city of Mumbai, India. Their surface morphologies were studied by Scanning Electron Microscopy (SEM) and their identity was confirmed using the standard 16S rRNA sequencing method. Upon performing several repetitive toxicity assays of these three strains on the laboratory cultured third instar stage of Culex quinquefasciatus larvae, showed differential toxicities from a minimum of 20% (LC50: 59.6 CFU/ml), intermediate 35% (LC50: 48.4 CFU/ml) and a maximum of 60% (LC50: 35.7 CFU/ml). To justify the data in all the three similar strains of Enterococcus durans, we followed the differential proteomics using LCMS 6540 UHD Accurate Mass QTOF and differential metabolomics approach using both LCMS 6540 UHD Accurate Mass QTOF and 1H-NMR. The presence and significance of the obtained toxins were studied to elucidate the plausible reason for showing differential toxicities. This work helped in identifying Enterococcus durans as a new, potential and alternative strain to the Bacillus species in terms of mosquito larvicidal toxicity against Culex quinquefasciatus.
Attention strengthens across-trial pre-stimulus phase coherence in visual cortex, enhancing stimulus processing
Authors: Zareian Behzad , Maboudi Kourosh , Daliri Mohammad Reza , Abrishami Moghaddam Hamid , Treue Stefan , Esghaei Moein 
Published: March 2020

AbstractAttention selectively routes the most behaviorally relevant information from the stream of sensory inputs through the hierarchy of cortical areas. Previous studies have shown that visual attention depends on the phase of oscillatory brain activities. These studies mainly focused on the stimulus presentation period, rather than the pre-stimulus period. Here, we hypothesize that selective attention controls the phase of oscillatory neural activities to efficiently process relevant information. We document an attentional modulation of pre-stimulus inter-trial phase coherence (a measure of deviation between instantaneous phases of trials) of low frequency local field potentials (LFP) in visual area MT of macaque monkeys. Our data reveal that phase coherence increases following a spatial cue deploying attention towards the receptive field of the recorded neural population. We further show that the attentional enhancement of phase coherence is positively correlated with the modulation of the stimulus-induced firing rate, and importantly, a higher phase coherence is associated with a faster behavioral response. These results suggest a functional utilization of intrinsic neural oscillatory activities for an enhanced processing of upcoming stimuli.
Hybrid of Graphene based on quaternary Cu2ZnNiSe4 –WO3 Nanorods for Counter Electrode in Dye-sensitized Solar Cell Application
Authors: Oh Won Chun , Cho Kwang Youn , Jung Chong Hun , Areerob Yonrapach 
Published: March 2020

AbstractA novel nanohybrid of graphene-based Cu2ZnNiSe4 with WO3 nanorods (G-CZNS@W) was successfully synthesized via a simple hydrothermal method to use as a counter electrode (CE) for dye-sensitized solar cells (DSSCs). The characterization technique confirmed the structural and morphologies of the G-CZNS@W nanohybrid, which could show rapid electrons transfer pathway through the WO3 nanorods. Moreover, the as-fabricated G-CZNS@W nanohybrid exhibited synergetic effect between G-CZNS and a WO3 nanorod, which could affect the electrocatalytic activity towards triiodide reaction. The nanohybrid exhibits an excellent photovoltaic performance of 12.16%, which is higher than that of the standard Pt electrode under the same conditions. The G-CZNS@W nanohybrid material as CE thus offers a promising low-cost Pt-free counter electrode for DSSC.
Bipolar Hemiarthroplasty should not be selected as the primary option for intertrochanteric fractures in elderly patients
Authors: Huang Jincheng , Shi Yanxin , Pan Weiyu , Wang Zhen , Dong Yonghui , Bai Yu , Wang Aiguo , Zhao Yongqiang , Zheng Jia , Lian Hongkai 
Published: March 2020

AbstractIntertrochanteric fractures (ITFs) in the elderly are still a big challenge for clinical doctors. Although proximal femoral nail antirotation (PFNA) and bipolar hemiarthroplasty (BPH) are selected by most of the orthopaedic surgeons for elderly ITFs patients, there is still no consensus on the superiority of PFNA and BPH for ITFs in elderly. In this study, we hypothesized that BPH should not be selected as the primary option for ITFs in elderly patients, and analyzed clinical data of 202 elderly ITFs patients aged 80 years or more treated with PFNA (Group A) and BPH (Group B) to compare the early outcome of PFNA and BPH for ITFs in elderly patients aged 80 years or more. We found that operation time and blood loss during surgery in group A are less than in Group B. Time of weight bearing after operation in Group A is longer than in Group B. Incidence of complications 2 weeks after operation in Group A is 9.29% less than 25.81% in Group B (χ2 = 9.539, p = 0.002). Mortality rates 12 months after operation in Group A is 11.43% similar with 19.35% in Group B (χ2 = 2.261, p = 0.133). Harris Hip Score 12 months after operation in Group A is 68.00 ± 29.11 points similar with 65.73 ± 33.29 points in Group B (t = 0.490, p = 0.625). Therefore, for elderly ITFs patients aged 80 years or more, BPH should not be selected as the primary option for ITFs in elderly patients.
Delineating elastic properties of kinesin linker and their sensitivity to point mutations
Authors: Świątek Michał , Gudowska-Nowak Ewa 
Published: March 2020

AbstractWe analyze free energy estimators from simulation trials mimicking single-molecule pulling experiments on a neck linker of a kinesin motor. For that purpose, we have performed a version of steered molecular dynamics (SMD) calculations. The sample trajectories have been analyzed to derive distribution of work done on the system. In order to induce stretching of the linker, we have applied a constant pulling force to the molecule and allowed for a subsequent relaxation of its structure. The use of fluctuation relations (FR) relevant to non-equilibrium systems subject to thermal fluctuations allows us to assess the difference in free energy between stretched and relaxed conformations. To further understand effects of potential mutations on elastic properties of the linker, we have performed similar in silico studies on a structure formed of a polyalanine sequence (Ala-only) and on three other structures, created by substituting selected types of amino acid residues in the linker’s sequence with alanine (Ala) ones. The results of SMD simulations indicate a crucial role played by the Asparagine (Asn) and Lysine (Lys) residues in controlling stretching and relaxation properties of the linker domain of the motor.
Selectivity of the botanical compounds to the pollinators Apis mellifera and Trigona hyalinata (Hymenoptera: Apidae)
Authors: da Silva Isabel Moreira , Zanuncio José Cola , Brügger Bruno Pandelo , Soares Marcus Alvarenga , Zanuncio Antônio José Vinha , Wilcken Carlos Frederico , Tavares Wagner de Souza , Serrão José Eduardo , Sediyama Carlos Sigueyuki 
Published: March 2020

AbstractThe toxicity of essential oils that can be used in insect pest management to pollinators needs further studies. Apis mellifera Linnaeus and Trigona hyalinata (Lepeletier) (Hymenoptera: Apidae) foragers were exposed by three pathways to ginger, mint, oregano and thyme essential oils to provide their LC50, LD50 and LC90, LD90. Oregano and thyme were more toxic through contact and topically for A. mellifera while the toxicity of mint and ginger was lower. Trigona hyalinata was more tolerant to the essential oils than A. mellifera. In the walking test, the area was treated (totally or partially) with sub-doses (LC50) obtained via contact. The area fully treated with oregano reduced the distance traveled and the movement speed increased the number of stops by A. mellifera. Similar results were observed for T. hyalinata with oregano and thyme oils. Apis mellifera showed irritability remaining shorter time in the area partially treated with ginger, mint and thyme essential oils while T. hyalinata had similar behavior with ginger and thyme. Essential oils did not repel A. mellifera or T. hyalinata, but those of ginger, mint and thyme reduced the time spent by A. mellifera in areas treated with sublethal doses. Oregano and thyme essential oils reduced the survival, mainly, of A. mellifera, while ginger and mint were selective for both pollinators.
Integrating sol-gel and carbon dots chemistry for the fabrication of fluorescent hybrid organic-inorganic films
Authors: Mura Stefania , Ludmerczki Róbert , Stagi Luigi , Garroni Sebastiano , Carbonaro Carlo Maria , Ricci Pier Carlo , Casula Maria Francesca , Malfatti Luca , Innocenzi Plinio 
Published: March 2020

AbstractHighly fluorescent blue and green-emitting carbon dots have been designed to be integrated into sol-gel processing of hybrid organic-inorganic materials through surface modification with an organosilane, 3-(aminopropyl)triethoxysilane (APTES). The carbon dots have been synthesised using citric acid and urea as precursors; the intense fluorescence exhibited by the nanoparticles, among the highest reported in the scientific literature, has been stabilised against quenching by APTES. When the modification is carried out in an aqueous solution, it leads to the formation of silica around the C-dots and an increase of luminescence, but also to the formation of large clusters which do not allow the deposition of optically transparent films. On the contrary, when the C-dots are modified in ethanol, the APTES improves the stability in the precursor sol even if any passivating thin silica shell does not form. Hybrid films containing APTES-functionalized C-dots are transparent with no traces of C-dots aggregation and show an intense luminescence in the blue and green range.
Specific and segregated changes to the functional connectome evoked by the processing of emotional faces: A task-based connectome study
Authors: Markett Sebastian , Jawinski Philippe , Kirsch Peter , Gerchen Martin F. 
Published: March 2020

AbstractThe functional connectome is organized into several separable intrinsic connectivity networks (ICNs) that are thought to be the building blocks of the mind. However, it is currently not well understood how these networks are engaged by emotionally salient information, and how such engagement fits into emotion theories. The current study assessed how ICNs respond during the processing of angry and fearful faces in a large sample (N = 843) and examined how connectivity changes relate to the ICNs. All ICNs were modulated by emotional faces and showed functional interactions, a finding which is in line with the “theory of constructed emotions” that assumes that basic emotion do not arise from separable ICNs but from their interplay. We further identified a set of brain regions whose connectivity changes during the tasks suggest a special role as “affective hubs” in the brain. While hubs were located in all ICNs, we observed high selectivity for the amygdala within the subcortical network, a finding which also fits into “primary emotion” theory. The topology of hubs corresponded closely to a set of brain regions that has been implicated in anxiety disorders, pointing towards a clinical relevance of the present findings. The present data are the most comprehensive mapping of connectome-wide changes in functionally connectivity evoked by an affective processing task thus far and support two competing views on how emotions are represented in the brain, suggesting that the connectome paradigm might help with unifying the two ideas.
Eye contact boosts the reflexive component of overt gaze following
Authors: Dalmaso Mario , Alessi Giada , Castelli Luigi , Galfano Giovanni 
Published: March 2020

AbstractEstablishing eye contact with an individual can subsequently lead to a stronger gaze-mediated orienting effect. However, studies exploring this phenomenon have, so far, only assessed manual responses and focused on covert attention – namely, without eye movements. Here, in two experiments, we explored for the first time whether eye contact can also impact on overt attention in an oculomotor task. This approach has two main advantages, in that it relies on more sensitive, online measures of attention allocation and it better mimics real life settings. Participants performed leftwards and rightwards eye movements in response to a central cue. Furthermore, a task-irrelevant central face established – or not – eye contact with the participant, and then averted its gaze either leftwards or rightwards. Hence, eye movement direction was either congruent or incongruent with that of the gaze stimulus. In both experiments, a gaze following behaviour emerged – specifically, smaller saccadic latencies and a greater accuracy emerged on congruent than on incongruent trials – but its magnitude was not modulated by eye contact. However, in Experiment 2 – in which the different eye contact conditions were presented intermixed rather than blocked, thus making eye contact contextually salient – eye contact led to an overall decrement of saccadic latencies and enhanced the reflexive component of gaze following. Taken together, these results provide novel evidence indicating that eye contact can impact on both eye movements programming and overt gaze following mechanisms, at least when eye contact is made contextually salient.
Usefulness of melatonin as complementary to chemotherapeutic agents at different stages of the angiogenic process
Authors: González-González Alicia , González Alicia , Rueda Noemi , Alonso-González Carolina , Menéndez Javier Menéndez , Martínez-Campa Carlos , Mitola Stefania , Cos Samuel 
Published: March 2020

AbstractChemotherapeutics are sometimes administered with drugs, like antiangiogenic compounds, to increase their effectiveness. Melatonin exerts antitumoral actions through antiangiogenic actions. We studied if melatonin regulates the response of HUVECs to chemotherapeutics (docetaxel and vinorelbine). The inhibition that these agents exert on some of the processes involved in angiogenesis, such as, cell proliferation, migratory capacity or vessel formation, was enhanced by melatonin. Regarding to estrogen biosynthesis, melatonin impeded the negative effect of vinorelbine, by decreasing the activity and expression of aromatase and sulfatase. Docetaxel and vinorelbine increased the expression of VEGF-A, VEGF-B, VEGF-C, VEGFR-1, VEGFR-3, ANG1 and/or ANG-2 and melatonin inhibited these actions. Besides, melatonin prevented the positive actions that docetaxel exerts on the expression of other factors related to angiogenesis like JAG1, ANPEP, IGF-1, CXCL6, AKT1, ERK1, ERK2, MMP14 and NOS3 and neutralized the stimulating actions of vinorelbine on the expression of FIGF, FGFR3, CXCL6, CCL2, ERK1, ERK2, AKT1, NOS3 and MMP14. In CAM assay melatonin inhibited new vascularization in combination with chemotherapeutics. Melatonin further enhanced the chemotherapeutics-induced inhibition of p-AKT and p-ERK and neutralized the chemotherapeutics-caused stimulatory effect on HUVECs permeability by modifying the distribution of VE cadherin. Our results confirm that melatonin blocks proangiogenic and potentiates antiangiogenic effects induced by docetaxel and vinorelbine enhancing their antitumor effectiveness.
Double power-law viscoelastic relaxation of living cells encodes motility trends
Authors: de Sousa J. S. , Freire R. S. , Sousa F. D. , Radmacher M. , Silva A. F. B. , Ramos M. V. , Monteiro-Moreira A. C. O. , Mesquita F. P. , Moraes M. E. A. , Montenegro R. C. , Oliveira C. L. N. 
Published: March 2020

AbstractLiving cells are constantly exchanging momentum with their surroundings. So far, there is no consensus regarding how cells respond to such external stimuli, although it reveals much about their internal structures, motility as well as the emergence of disorders. Here, we report that twelve cell lines, ranging from healthy fibroblasts to cancer cells, hold a ubiquitous double power-law viscoelastic relaxation compatible with the fractional Kelvin-Voigt viscoelastic model. Atomic Force Microscopy measurements in time domain were employed to determine the mechanical parameters, namely, the fast and slow relaxation exponents, the crossover timescale between power law regimes, and the cell stiffness. These cell-dependent quantities show strong correlation with their collective migration and invasiveness properties. Beyond that, the crossover timescale sets the fastest timescale for cells to perform their biological functions.
Development of an experimental method of systematically estimating protein expression limits in HEK293 cells
Authors: Mori Yoshihiro , Yoshida Yuki , Satoh Ayano , Moriya Hisao 
Published: March 2020

AbstractProtein overexpression sometimes causes cellular defects, although the underlying mechanism is still unknown. A protein’s expression limit, which triggers cellular defects, is a useful indication of the underlying mechanism. In this study, we developed an experimental method of estimating the expression limits of target proteins in the human embryonic kidney cell line HEK293 by measuring the proteins’ expression levels in cells that survived after the high-copy introduction of plasmid DNA by which the proteins were expressed under a strong cytomegalovirus promoter. The expression limits of nonfluorescent target proteins were indirectly estimated by measuring the levels of green fluorescent protein (GFP) connected to the target proteins with the self-cleaving sequence P2A. The expression limit of a model GFP was ~5.0% of the total protein, and sustained GFP overexpression caused cell death. The expression limits of GFPs with mitochondria-targeting signals and endoplasmic reticulum localization signals were 1.6% and 0.38%, respectively. The expression limits of four proteins involved in vesicular trafficking were far lower compared to a red fluorescent protein. The protein expression limit estimation method developed will be valuable for defining toxic proteins and consequences of protein overexpression.
Facile generation of giant unilamellar vesicles using polyacrylamide gels
Authors: Parigoris Eric , Dunkelmann Daniel L. , Murphy Allan , Wili Nino , Kaech Andres , Dumrese Claudia , Jimenez-Rojo Noemi , Silvan Unai 
Published: March 2020

AbstractGiant unilamellar vesicles (GUVs) are model cell-sized systems that have broad applications including drug delivery, analysis of membrane biophysics, and synthetic reconstitution of cellular machineries. Although numerous methods for the generation of free-floating GUVs have been established over the past few decades, only a fraction have successfully produced uniform vesicle populations both from charged lipids and in buffers of physiological ionic strength. In the method described here, we generate large numbers of free-floating GUVs through the rehydration of lipid films deposited on soft polyacrylamide (PAA) gels. We show that this technique produces high GUV concentrations for a range of lipid types, including charged ones, independently of the ionic strength of the buffer used. We demonstrate that the gentle hydration of PAA gels results in predominantly unilamellar vesicles, which is in contrast to comparable methods analyzed in this work. Unilamellarity is a defining feature of GUVs and the generation of uniform populations is key for many downstream applications. The PAA method is widely applicable and can be easily implemented with commonly utilized laboratory reagents, making it an appealing platform for the study of membrane biophysics.
Ground state and collective excitations of a dipolar Bose-Einstein condensate in a bubble trap
Authors: Diniz Pedro C. , Oliveira Eduardo A. B. , Lima Aristeu R. P. , Henn Emanuel A. L. 
Published: March 2020

AbstractWe consider the ground state and the collective excitations of dipolar Bose-Einstein condensates in a bubble trap, i.e., a shell-shaped spherically symmetric confining potential. By means of an appropriate Gaussian ansatz, we determine the ground-state properties in the case where the particles interact by means of both the isotropic and short-range contact and the anisotropic and long-range dipole-dipole potential in the thin-shell limit. Moreover, with the ground state at hand, we employ the sum-rule approach to study the monopole, the two-, the three-dimensional quadrupole as well as the dipole modes. We find situations in which neither the virial nor Kohn’s theorem can be applied. On top of that, we demonstrate the existence of anisotropic particle density profiles, which are absent in the case with repulsive contact interaction only. These significant deviations from what one would typically expect are then traced back to both the anisotropic nature of the dipolar interaction and the novel topology introduced by the bubble trap.
Area-specific economic status should be regarded as a vital factor affecting the occurrence, development and outcome of cervical cancer
Authors: Li Zichao , Wu Haozhi , Yi Xiaowei , Tian Fangyu , Zhang Xiyang , Zhou Haikun , Liu Biqing , Lu Zhenhua , Wang Jing , Jiang Dongbo , Shang Lei , Yang Kun 
Published: March 2020

AbstractFor patients with cervical cancer, despite the incidence and mortality rates have been declining in recent years, due to its huge population base, cervical cancer has always been a serious public health problem. Our research placed emphasis on the indices greatly associated with overall area-specific social economic status, making up for the defects of traditional research which only pay attention to the situation of some specific disease or patients’ individual social status. A total of 39160 women identified cervical cancer were concluded in our study from the Surveillance, Epidemiology, and End Results (SEER) 18 Program data between 1980 and 2014. With improving the area-specific social economic factors in recent years, the occurrence and prognosis of cervical cancer showed different variation patterns respectively. Some states like California and Georgia for their better economic status and more healthcare investment by local medical institution, population there showed a lower prevalence, incidence, more timely diagnosis, effective treatment, and better prognosis. According to our study, we aimed to give a scientific interpretation on how the area-specific social economic factors affect the disease situation at the macro level and help local medical institution make advisable decisions for controlling cervical cancer.