bluuebunny/crossref_metadata_2025_split huggingface dataset:
HR Journey in a Family-owned Business: The Evolved Owner Makes a Difference
Authors: Bhargava Pankaj 
Published: January 2020

What does it take for a family-run business that has a legacy of generations to become known as a best-in-class professionally run organisation—preferred as a workplace by talent, creating leaders for the industry and ambassadorship among employees and alumni? This is the HR journey of Marico, led by an evolved owner, Mr Harsh Mariwala. In this article, we discuss four key HR practices of attracting talent, induction and probation of recruited talent, building accountability and developing talent. These practices work on principles that have withstood the test of time, evolving with the changing context of the business and external environment. These also represent the evolution of the HR role over a period of time. The author then elaborates how a professional work culture got institutionalised in Marico.
RGL2 Deficiency Impairs Human Erythropoiesis By Altering Terminal Erythroid Differentiation and Apoptosis
Authors: Lesmana Harry , Christakopoulos Georgios E , Risinger Mary , Sakthivel Haripriya , Niss Omar , Kalfa Theodosia A. 
Published: January 2020

Abstract
Several members of the Ras-GTPases have been shown to play major roles as molecular switches in various signaling pathways in hematopoietic cells. The roles of Ras/MAPKs/ERK signaling pathways in EPO-induced erythroid differentiation have been explored in the past on animal models. Recently, the Ras/RalGEFs/Ral pathway has been a focus of research in oncology given its roles in tumorigenesis. However, its role in normal human erythropoiesis is poorly understood. The seven RalGEF family members are guanine nucleotide exchange factors with unique as well as overlapping roles. They bridge activated Ras to activation of RalA and RalB while they have also been shown to promote Akt activation. Recently, we identified and characterized a homozygous splicing variant (c.1580+4T&gt;A) in RGL2 (Ral Guanine Nucleotide Dissociation Stimulator-Like 2) gene, one of the RalGEFs, in a 16 month-old female patient with a recessive form of familial syndromic pancytopenia, more severe in the erythroid lineage with transfusion-dependent anemia, hepatosplenomegaly, liver fibrosis, and cardiac dysfunction. We verified by RT-PCR that this splicing variant was associated by a decrease in the RGL2 expression levels by 70-96% in the white blood cells and reticulocytes of the patient. We hypothesized that decreased RGL2 expression leads to defective human erythropoiesis by altering terminal erythroid differentiation, erythroblast cell cycle progression and/or apoptosis.
To test our hypothesis, we transduced human bone marrow derived CD34+ cells (donated by healthy volunteers under an IRB-approved research protocol in our institution) with lentivirus encoding shRNA targeting RGL2 mRNA and then cultured them in a previously validated 3-phase culture system that recapitulates erythropoiesis ex vivo. A scrambled shRNA sequence was used as control. The efficiency of the shRNAs used to knock-down (KD) RGL2 expression was initially validated by qRT-PCR after transduction of K562 cells. Our study demonstrated that knockdown of RGL2 expression resulted in decreased erythroid cell growth in ex vivo erythropoiesis accompanied by increased apoptosis. We further showed that knockdown of RGL2 expression also led to delayed terminal erythroid differentiation by flow cytometric analysis using glycophorin A (GPA), band 3, and α4-integrin as markers of maturing erythroblasts. This finding was corroborated by morphology studies using Wright staining in cytospins from RGL2-KD cultures and control. Together, our studies indicate an essential role for RGL2 and the Ras/RGL2/Ral pathway in human erythropoiesis, affecting both terminal erythroid differentiation and apoptosis. Our findings not only provide insights into regulation of normal erythropoiesis but also indicate that RGL2 may be a novel candidate gene associated with inherited bone marrow failure syndromes.

Disclosures
No relevant conflicts of interest to declare.
Cytotoxycity and antiplasmodial activity of phenolic derivatives from Albizia zygia (DC.) J.F. Macbr. (Mimosaceae)
Authors: Koagne Romeol Romain , Annang Frederick , Cautain Bastien , Martín Jesús , Pérez-Moreno Guiomar , Bitchagno Gabin Thierry M. , González-Pacanowska Dolores , Vicente Francisca , Simo Ingrid Konga , Reyes Fernando , Tane Pierre 
Published: January 2020

Abstract
Background
The proliferation and resistance of microorganisms area serious threat against humankind and the search for new therapeutics is needed. The present report describes the antiplasmodial and anticancer activities of samples isolated from the methanol extract of Albizia zygia (Mimosaseae).

Material
The plant extract was prepared by maceration in methanol. Standard chromatographic, HPLC and spectroscopic methods were used to isolate and identify six compounds (1–6). The acetylated derivatives (7–10) were prepared by modifying 2-O-β-D-glucopyranosyl-4-hydroxyphenylacetic acid and quercetin 3-O-α-L-rhamnopyranoside, previously isolated from A. zygia (Mimosaceae). A two-fold serial micro-dilution method was used to determine the IC50s against five tumor cell lines and Plasmodium falciparum.

Results
In general, compounds showed moderate activity against the human pancreatic carcinoma cell line MiaPaca-2 (10 < IC50 < 20 μM) and weak activity against other tumor cell lines such as lung (A-549), hepatocarcinoma (HepG2) and human breast adenocarcinoma (MCF-7and A2058) (IC50 > 20 μM). Additionally, the two semi-synthetic derivatives of quercetin 3-O-α-L-rhamnopyranoside exhibited significant activity against P. falciparum with IC50 of 7.47 ± 0.25 μM for compound 9 and 6.77 ± 0.25 μM for compound 10, higher than that of their natural precursor (IC50 25.1 ± 0.25 μM).

Conclusion
The results of this study clearly suggest that, the appropriate introduction of acetyl groups into some flavonoids could lead to more useful derivatives for the development of an antiplasmodial agent.
Mesenchymal stem cells promote human melanocytes proliferation and resistance to apoptosis through PTEN pathway in vitiligo
Authors: Zhu Lifei , Lin Xi , Zhi Lin , Fang Yushan , Lin Keming , Li Kai , Wu Liangcai 
Published: January 2020

Abstract
Background
Vitiligo is an acquired chronic and recurrent skin disease that causes a depigmentation disorder, resulting in selective destruction of melanocytes (MC). However, the mechanism that leads to melanocyte dysfunction and death remains unclear.

Methods
We performed RNA sequencing, immunohistochemistry, and immunoblotting to characterize the patterns of phosphatase and tensin homolog (PTEN)/phosphatidylinositol 3 kinase (PI3K)/protein kinase B (AKT) pathway activation in vitiligo. We also cocultured primary melanocytes with mesenchymal stem cells (MSCs) in a Transwell system to explore how MSCs inhibit the PTEN/PI3K/AKT pathway in melanocytes.

Results
We identified that vitiligo normal-lesional junction skin presented with high expression of PTEN, which led to the inhibition of AKT phosphorylation (p-AKT) at S-473. Furthermore, PTEN overexpression led to oxidative stress-induced apoptosis in melanocytes. Coculturing with MSCs enhanced the cell proliferation of human melanocytes and repressed PTEN expression, which inhibited oxidative stress-induced apoptosis.

Conclusion
We report that vitiligo patients present with high PTEN expression, which may play a role in the impairment of melanocytes. Furthermore, our study provides evidence that MSCs target the PTEN/PI3K/AKT pathway to regulate cell proliferation and apoptosis in human melanocytes, indicating that MSCs may serve as a promising therapy for vitiligo.
Recombinant expression and biochemical characterization of a novel keratinase BsKER71 from feather degrading bacterium Bacillus subtilis S1-4
Authors: Yong Bin , Fei Xueting , Shao Huanhuan , Xu Pan , Hu Youwen , Ni Weimin , Xiao Qiuju , Tao Xiang , He Xinyi , Feng Hong 
Published: January 2020

AbstractBacillus subtilis S1-4, isolated from chicken feather could efficiently degrade feathers by secreting several extracellular proteases. In order to get insight into the individual protease involved in keratin hydrolysis, a keratinase designed as BsKER71 was cloned and expressed in Bacillus subtilis WB600. In silico analysis revealed that BsKER71 protein contained a mature protein of 36.1 kDa. Further, purified BsKER71 could hydrolyze a variety of natural proteins, such as fibrous protein, collagen protein, casein, keratin and bovine serum albumin. In addition, this keratinase exhibited high enzyme activity in a wide range of pH and optimal pH of 10.0 and 9.0 in the hydrolysis of casein and keratin, respectively. Similarly, the optimal temperature was 55 °C and 50 °C for the hydrolysis of above two substrates, respectively. The hydrolytic activity was significantly inhibited by phenylmethanesulfonyl fluoride (PMSF), indicating the presence of serine residue in the active site. Moreover, ethylenediaminetetraacetic acid (EDTA) and phenanthroline moderately inhibited the hydrolytic activity. The catalytic activity was stimulated by Mg2+ and Ca2+, but greatly inhibited by Cu2+. Furthermore, several chemicals exhibited different effects on the hydrolysis of casein and keratin by BsKER71. These results provided a better understanding of BsKER71 from feather degrading bacterium B. subtilis S1-4.
Sagittal alignment in an MR-TRUS fusion biopsy using only the prostate contour in the axial image
Authors: Igarasihi Riki , Koizumi Norihiro , Nishiyama Yu , Tomita Kyohei , Shigenari Yuka , Shoji Sunao 
Published: January 2020

Abstract
Purpose
This paper examines the feasibility of automated alignment in sagittal direction in MR-TRUS fusion biopsy of the prostate by comparing the prostate contour in axial images between different modalities. In the treatment of prostate cancer, an important factor affecting the prognosis of patients is focal therapy of cancer within the prostate. Therefore, MR-TRUS fusion biopsy of the prostate is attracting attention as one of the most effective localization techniques. Because the accuracy of this biopsy is highly dependent on the doctor performing it, automation should reduce variability in diagnostic performance.

Method
The MR image is scaled to the same scale as the TRUS image, and the contours of the prostate on the MR and TRUS images are compared in polar coordinates. In addition, this method makes it possible to perform a robust comparison against deformation by comparing specific angle ranges. It is also possible to improve the accuracy of error calculation by accumulating contour data.

Result
The axial image selected by the proposed method using the prostate contour obtained from the doctor-labeled segmentation image has an error of about 4 mm in the sagittal direction on average compared to the axial image selected by the doctor did. Furthermore, using the inaccurate prostate contours obtained by performing segmentation with U-Net only slightly reduced the accuracy. In addition, it was found that alignment accuracy is improved by using the angler weight.

Conclusion
It has been shown that sagittal alignment can be performed with some degree of accuracy using only axial images. Also, the angular weight values used indicate that when comparing axial images, it may be an important factor in determining the same axial cross section to compare the parts that deform due to probe pressure.
Metachronous liver metastases after long-term follow-up of endoscopic resection for rectal neuroendocrine neoplasms: a report of three cases
Authors: Hane Yuma , Tsuchikawa Takahiro , Tanaka Kimitaka , Nakanishi Yoshitsugu , Asano Toshimichi , Noji Takehiro , Kurashima Yo , Ebihara Yuma , Murakami Soichi , Nakamura Toru , Okamura Keisuke , Takeuchi Satoshi , Shichinohe Toshiaki , Hirano Satoshi 
Published: January 2020

Abstract
Background
Rectal neuroendocrine neoplasms (NENs) are rare, but their incidence has increased in recent years. The metastasis rate is low in cases of a tumor diameter < 1 cm or depth of invasion lower than the submucosa; therefore, the European Neuroendocrine Tumor Society (ENETS) and the North American Neuroendocrine Tumor Society (NANETS) consensus guidelines recommend endoscopic resection. Since little has been reported on the long-term prognosis of endoscopic resection for rectal NEN, consensus is lacking regarding the follow-up period after endoscopic resection.

Case presentation
Here, we report three cases of metachronous liver metastasis after long-term follow-up of endoscopic mucosal resection (EMR) for rectal NEN. The pathological findings indicated a depth lower than the submucosa and complete radical resection in all cases and lymphovascular invasion in only one case. All three cases showed metachronous multiple liver metastases after 9–13 years of follow-up for EMR, despite achieving complete resection and without muscular invasion.

Conclusions
Metachronous liver metastases may occur after long interval following endoscopic resection; thus, long-term follow-up is necessary after endoscopic resection for rectal NEN.
Effect of different fermentation strategies on Bacillus thuringiensis cultivation and its toxicity towards the bagworm, Metisa plana Walker (Lepidoptera: Psychidae)
Authors: Masri Mohamed Mazmira Mohd , Ariff Arbakariya Bin 
Published: January 2020

AbstractThe effect of batch and fed-batch fermentation on the cultivation performance of Bacillus thuringiensis was investigated using a 5-l stirred tank bioreactor. Significantly higher viable cell count (> 1.5 × 1012 CFU/ml) was obtained in the fed-batch compared to batch fermentation (1.4 × 1012 CFU/ml). Glucose feeding during the fermentation seemed to enhance cell growth but failed to enhance the sporulation rate. It was found that sporulation and δ-endotoxin synthesis in fed-batch fermentation could be enhanced by the application of optimal dissolved oxygen tension (DOT) control strategy without affecting the cell growth. Fed-batch cultivation with feeding at the exponential growth phase where the DOT was switched from 80 to 40% at 12 h of cultivation recorded the highest spore count of 7.1 × 1011 spore/ml. Cultures obtained from batch cultivation, as well as fed-batch cultivation with feeding at lag or exponential growth phase and the application of optimal DOT control strategy, recorded the presence of δ-endotoxin; however, none was detected in intermittent fed-batch fermentation. Bioassay data against the bagworm Metisa plana Walker (Lepidoptera: Psychidae) recorded the highest corrected mortality (80%) at 7 days of treatment (DAT), using the culture obtained from fed-batch cultivation with feeding during the exponential growth phase, and the DOT was switched from 80 to 40% at 12 h of cultivation. It is important to note that all cultures containing δ-endotoxin exhibited 100% mortality towards M. plana at 14 DAT.
A second-take on the role of science: the case for applying public administration theory to natural resource management
Authors: Brock Philippa M. , Tan Daniel K. Y. 
Published: January 2020

Abstract
Background
Natural resource management theory has been informed by disciplines such as ecology, agricultural science, economics and engineering; but much less so by the literature of planning and public administration. This paper demonstrates that applying an incremental view to what is traditionally seen as a rational-comprehensive discipline provides insights into resource management theory and practice, including adaptive management.

Results
We show how current theory and practice in natural resource management are not aligned to the degree that is routinely presumed. In particular, the prevailing focus on optimality creates conflicts, especially during democratic decision-making, whereas adopting a participatory-incremental hybrid approach should provide a superior solution, which is discussed here in the context of Life Cycle Assessment by resource managers.

Conclusion
Resource management tends to be both incremental and participatory; and whilst decisions are strongly informed by science, on reflection, the process of formulating decisions is rarely rational-comprehensive. We suggest greater retrospective analysis of resource management decisions at a theoretical level. This may result in adoption of a theoretical framework which better supports practice, a reduction in tensions between those trained in the arts and sciences; and more ‘freedom’ in practice, through a softening of the focus on optimality.
Magnetic resonance spectroscopy in pediatric brain tumors: how to make a more confident diagnosis
Authors: Attia Noha M. , Sayed Samy Abdel Aziz , Riad Khaled Fathy , Korany Ghada Mahmoud 
Published: January 2020

Abstract
Background
Non-invasive diagnosis of pediatric brain tumors can be challenging due to diverse tumor pathologies and similar imaging appearances. Magnetic resonance spectroscopy (MRS), when combined with high spatial resolution anatomic imaging obtained with conventional magnetic resonance imaging (MRI), provides metabolic information within the lesion as well as the surrounding tissue. The differentiation of neoplastic from non-neoplastic lesions and low-grade from high-grade neoplasms is essential for determining the choice of treatment and the best treatment plan. We aimed to measure specific metabolic ratios and evaluate metabolic profiles of various lesions by MRS to assist in making a more confident diagnosis.

Results
The choline/creatine (Cho/Cr), choline/N-acetylaspartate (Cho/NAA), and Cho/NAA+Cr ratios all had statistically significant values for the differentiation between neoplastic and non-neoplastic lesions at cutoffs 1.8, 2, and 0.8 respectively. The Cho/NAA, Cho/Cr, Cho/NAA+Cr, and myo-inositol/creatine (mI/Cr) ratios all had statistically significant values for the differentiation of high-grade from low-grade neoplasms at cutoffs 3.3, 3.5, 1.3, and 1.5 respectively. The presence of a lipid lactate peak was only significant for differentiating high-grade from low-grade neoplasms. Medulloblastomas, diffuse pontine gliomas, and choroid plexus carcinoma all showed characteristic metabolic profiles on MRS. Metastasis showed lower Cho/NAA and Cho/Cr ratios outside the tumor margin than high-grade neoplasms.

Conclusion
The use of certain metabolite ratios with high sensitivity and specificity to distinguish neoplastic from non-neoplastic lesions and low-grade from high-grade neoplasms while assessing the metabolic profile of the lesion aids in the non-invasive diagnosis of pediatric brain tumors. MRS facilitates earlier treatment planning by determining tumor spatial extent and predicting tumor behavior with potential to solve sampling problems of inaccessible and heterogenous lesions as well as unnecessary sampling of benign lesions.
In The Eye of the Beholder – Attitudes towards Visual Poetry in Latvian Literature
Authors: Laizāns Mārtiņš 
Published: January 2020

Visual poetry in Latvian literature is still an extraordinary phenomenon. In this case, by extraordinary is meant its scarce appearance among the corpus of Latvian literary texts – only very few examples of it exist in Latvian literature even in the 21st century, though it has gained a visible presence in other literatures of the world since at least the era of Modernist poetry, and experienced in most cases a turn of critical attitude towards it from disregard or denial to acceptance and consideration. At first evaluated only as a pastime on the margins of serious literature, since Apollinaire it has evolved into a serious genre of poetry and art of its own, and is no longer considered a childish game. Given this situation in contemporary criticism, it is quite a peculiar situation that Latvian literature and literary criticism still does not pay adequate attention to it, thus visual poetry has stayed an outsider genre up to this day.
In this paper a concise historical account of Latvian visual poetry will be given and some examples of visual poetry from various decades of Latvian literature will be given, as well as quotations from Latvian literary critics and scholars regarding visual poetry will be provided, in order to illustrate the overall situation in Latvian visual poetry and the possible reasons why it is still being mostly neglected and disregarded both by poets/artists and critics, though there seems to be slight indications of a visual turn.
Metaficción historiográfica y autoficción: diferentes compromisos con la referencialidad en Estação das Chuvas de José Eduardo Agualusa y Soldados de Salamina de Javier Cercas
Authors: Cavaliere Mauro 
Published: January 2020

Historiographic metafiction and autofiction: different commitments with the referentiality in Estação das Chuvas by José Eduardo Agualusa and Soldiers of Salamina by Javier Cercas. This article offers a comparative analysis of the novels Estação das Chuvas (1996) by the Angolan writer José Eduardo Agualusa (born in 1960) and Soldados de Salamina (2001) of the Spaniard Javier Cercas (born in 1962). The two novels belong to different geographical and cultural contexts. Nevertheless, a common sensibility – due perhaps to the same generational affiliation or to the prevalence of topics in force in the 1990s – makes evident the emergence of both a historical theme and the presence of a subject involved in historical processes. Ultimately, in both novels, we come across a subject that makes history although in quite different ways: involved firsthand in historical events with tragic implications, in the case of Agualusa, and absorbed in a reflection on apparently distant events in the case of Cercas. However, the result of the emphasis on the presence of a subjectivity within historical processes causes the two novels to share a common element, that is, a double generic affiliation. Both Estação das Chuvas and Soldados de Salamina actually share semantic traits that make it possible to classify them at the same time as autofictional novels and historiographic metafictions.
Despite their common architectural matrix, the two novels represent two very different expressions within these genres. This manifests itself at different levels: first, the treatment of the autofictional character and, secondly, the treatment of the other characters. Through the analysis of the characters that populate these two novels, I will try to show how the two writers adopt divergent attitudes regarding the degree of referentiality in their works and how they end up proposing two different poetic options. In the analysis of the characters, I consider it useful to introduce a taxonomy that, in addition to including already existing types (referential, historical, fictitious characters), introduces other types hopefully useful to the study of the currently abundant number of fictions that, through an ambiguous narrative pact, are located between fiction and faction.