bluuebunny/crossref_metadata_2025_split huggingface dataset:
The capsaicin receptor TRPV1 is the first line defense protecting from acute non damaging heat: a translational approach
Authors: Rosenberger Daniela C. , Binzen Uta , Treede Rolf-Detlef , Greffrath Wolfgang 
Published: January 2020

AbstractBackgroundPain is the vital sense preventing tissue damage by harmful noxious stimuli. The capsaicin receptor TRPV1 is activated by noxious temperatures, however, acute heat pain is only marginally affected in mice after TRPV1 knockout but completely eliminated in mice lacking TRPV1 positive fibers. Exploring contribution of candidate signal transduction mechanisms to heat pain in humans needs translational models.MethodsWe used focused, non-damaging, short near-infrared laser heat stimuli (wavelength 1470/1475 nm) to study the involvement of TRPV1-expressing nerve fibers in the encoding of heat pain intensity. Human psychophysics (both sexes) were compared to calcium transients in native rat DRG neurons and heterologously expressing HEK293 cells.ResultsHeating of dermal and epidermal nerve fibers in humans with laser stimuli of ≥ 2.5 mJ (≥ 25 ms, 100 mW) induced pain that increased linearly as a function of stimulus intensity in double logarithmic space across two orders of magnitude and was completely abolished by desensitization using topical capsaicin. In DRG neurons and TRPV1-expressing HEK cells, heat sensitivity was restricted to capsaicin sensitive cells. Strength duration curves (2–10 ms range) and thresholds (DRGs 0.56 mJ, HEK cells 0.52 mJ) were nearly identical. Tachyphylaxis upon repetitive stimulation occurred in HEK cells (54%), DRGs (59%), and humans (25%).ConclusionTRPV1-expressing nociceptors encode transient non-damaging heat pain in humans, thermal gating of TRPV1 is similar in HEK cells and DRG neurons, and TRPV1 tachyphylaxis is an important modulator of heat pain sensitivity. These findings suggest that TRPV1 expressed in dermal and epidermal populations of nociceptors serves as first line defense against heat injury.
Overcoming barriers to evidence-based patient blood management: a restricted review
Authors: Delaforce Alana , Duff Jed , Munday Judy , Hardy Janet 
Published: January 2020

AbstractBackgroundBlood transfusions are associated with a range of adverse patient outcomes, including coagulopathy, immunomodulation and haemolysis, which increase the risk of morbidity and mortality. Consideration of these risks and potential benefits are necessary when deciding to transfuse. Patient blood management (PBM) guidelines exist to assist in clinical decision-making, but they are underutilised. Exploration of barriers to the implementation and utilisation of the PBM guidelines is required. This study aimed to identify common barriers and implementation strategies used to implement PBM guidelines, with a comparison against current expert opinion.MethodsA restricted review approach was used to identify the barriers to PBM guideline implementation as reported by health professionals and to review which implementation strategies have been used. Searches were undertaken in MEDLINE/PubMed, CINAHL, Embase, Scopus and the Cochrane library. The Consolidated Framework for Implementation Research (CFIR) was used to code barriers. The Expert Recommendations for Implementing Change (ERIC) tool was used to code implementation strategies, and subsequently, develop recommendations based on expert opinion.ResultsWe identified 14 studies suitable for inclusion. There was a cluster of barriers commonly reported: access to knowledge and information (n= 7), knowledge and beliefs about the intervention ( = 7) and tension for change (n= 6). Implementation strategies used varied widely (n= 25). Only one study reported the use of an implementation theory, model or framework. Most studies (n= 11) had at least 50% agreement with the ERIC recommendations.ConclusionsThere are common barriers experienced by health professionals when trying to implement PBM guidelines. There is currently no conclusive evidence to suggest which implementation strategies are most effective. Further research using validated implementation approaches and improved reporting is required.
Finnish gelsolin amyloidosis causes significant disease burden but does not affect survival: FIN-GAR phase II study
Authors: Schmidt Eeva-Kaisa , Mustonen Tuuli , Kiuru-Enari Sari , Kivelä Tero T. , Atula Sari 
Published: January 2020

Abstract
Background
Hereditary gelsolin (AGel) amyloidosis is an autosomal dominantly inherited systemic amyloidosis that manifests with the characteristic triad of progressive ophthalmological, neurological and dermatological signs and symptoms. The National Finnish Gelsolin Amyloidosis Registry (FIN-GAR) was founded in 2013 to collect clinical data on patients with AGel amyloidosis, including altogether approximately one third of the Finnish patients. We aim to deepen knowledge on the disease burden and life span of the patients using data from the updated FIN-GAR registry. We sent an updated questionnaire concerning the symptoms and signs, symptomatic treatments and subjective perception on disease progression to 240 members of the Finnish Amyloidosis Association (SAMY). We analyzed the lifespan of 478 patients using the relative survival (RS) framework.

Results
The updated FIN-GAR registry includes 261 patients. Symptoms and signs corresponding to the classical triad of ophthalmological (dry eyes in 93%; corneal lattice amyloidosis in 89%), neurological (numbness, tingling and other paresthesias in 75%; facial paresis in 67%), and dermatological (drooping eyelids in 86%; cutis laxa in 84%) manifestations were highly prevalent. Cardiac arrhythmias were reported by 15% of the patients and 5% had a cardiac pacemaker installed. Proteinuria was reported by 13% and renal failure by 5% of the patients. A total of 65% of the patients had undergone a skin or soft tissue surgery, 26% carpal tunnel surgery and 24% at least unilateral cataract surgery. As regards life span, relative survival estimates exceeded 1 for males and females until the age group of 70–74 years, for which it was 0.96.

Conclusions
AGel amyloidosis causes a wide variety of ophthalmological, neurological, cutaneous, and oral symptoms that together with repeated surgeries cause a clinically significant disease burden. Severe renal and cardiac manifestations are rare as compared to other systemic amyloidoses, explaining in part the finding that AGel amyloidosis does not shorten the life span of the patients at least for the first 75 years.
Shortening antibiotic duration in the treatment of acute cholangitis: rationale and study protocol for an open-label randomized controlled trial
Authors: Iwata Kentaro , Doi Asako , Oba Yuichiro , Matsuo Hiroo , Ebisawa Kei , Nagata Manabu , Nishimura Sho , Yoshimura Kenichi , Masuda Atsuhiro , Shiomi Hideyuki , Kodama Yuzo 
Published: January 2020

Abstract
Background
Antimicrobial therapy with appropriate biliary drainage is considered the standard of care for acute cholangitis, but the optimal duration of antimicrobial therapy remains unknown. Seven to 10 days of antimicrobial therapy are common for the treatment of acute cholangitis, but a recent retrospective cohort study suggested a shorter duration might be effective. A shorter duration of antimicrobial therapy can be beneficial in decreasing the length of hospital stay, improving patients’ quality of life, decreasing adverse effects, and even contributing to a decrease in the occurrence of antimicrobial resistance.

Methods/design
We will conduct a multi-centre, open-label, randomized, non-inferiority trial to compare short-course therapy (SCT) with conventional long-course therapy (LCT) in treating patients with acute cholangitis. SCT consists of 5-day intravenous antimicrobial therapy if the patients had clinical improvement, while at least 7 days of intravenous antibiotics will be provided to the LCT group. The primary outcome is clinical cure at 30 days after onset. Patients will be randomly assigned in an open-label fashion. A total sample size of 150 was estimated to provide a power of 80% with a one-sided α level of 2.5% and a non-inferiority margin of 10%.

Discussion
This trial is expected to reveal whether SCT is non-inferior to conventional LCT or not, and may provide evidence that one can shorten the treatment duration for acute cholangitis for the benefit of patients.

Trial registration
University Hospital Medical Information Network, UMIN000028382. Registered on 30 August 2017.
DNA barcode trnH-psbA is a promising candidate for efficient identification of forage legumes and grasses
Authors: Loera-Sánchez Miguel , Studer Bruno , Kölliker Roland 
Published: January 2020

Abstract
Objective
Grasslands are widespread ecosystems that fulfil many functions. Plant species richness (PSR) is known to have beneficial effects on such functions and monitoring PSR is crucial for tracking the effects of land use and agricultural management on these ecosystems. Unfortunately, traditional morphology-based methods are labor-intensive and cannot be adapted for high-throughput assessments. DNA barcoding could aid increasing the throughput of PSR assessments in grasslands. In this proof-of-concept work, we aimed at determining which of three plant DNA barcodes (rbcLa, matK and trnH-psbA) best discriminates 16 key grass and legume species common in temperate sub-alpine grasslands.

Results
Barcode trnH-psbA had a 100% correct assignment rate (CAR) in the five analyzed legumes, followed by rbcLa (93.3%) and matK (55.6%). Barcode trnH-psbA had a 100% CAR in the grasses Cynosurus cristatus, Dactylis glomerata and Trisetum flavescens. However, the closely related Festuca, Lolium and Poa species were not always correctly identified, which led to an overall CAR in grasses of 66.7%, 50.0% and 46.4% for trnH-psbA, matK and rbcLa, respectively. Barcode trnH-psbA is thus the most promising candidate for PSR assessments in permanent grasslands and could greatly support plant biodiversity monitoring on a larger scale.
Composite Epstein-Barr virus-positive mucosa-associated lymphoid tissue lymphoma and Epstein-Barr virus-negative diffuse large B-cell lymphoma in the parotid salivary gland of a patient with Sjögren’s syndrome and rheumatoid arthritis: a case report
Authors: Gorodetskiy Vadim R. , Probatova Natalya A. , Konovalov Dmitry M. , Ryzhikova Natalya V. , Sidorova Yulia V. , Sudarikov Andrey B. , Mukhortova Olga V. 
Published: January 2020

Abstract
Background
Epstein-Barr virus is associated with many human hematopoietic neoplasms; however, Epstein-Barr virus-positive mucosa-associated lymphoid tissue lymphoma is extremely rare. In routine clinical practice, detection of mucosa-associated lymphoid tissue lymphoma and diffuse large B-cell lymphoma in a tissue sample presumes a clonal relation between these neoplasms and that diffuse large B-cell lymphoma developed by transformation of the mucosa-associated lymphoid tissue lymphoma. However, evidence to support this presumption is sparse and controversial. Assessment of the clonal relationship of the lymphoid components of a composite lymphoma is important for understanding its pathogenesis and correct diagnosis.

Case presentation
We present an unusual case of composite lymphoma (Epstein-Barr virus-positive mucosa-associated lymphoid tissue lymphoma/Epstein-Barr virus-negative diffuse large B-cell lymphoma) in the parotid salivary gland of a 62-year-old Caucasian woman with Sjögren’s syndrome and rheumatoid arthritis. Simultaneous occurrence of mucosa-associated lymphoid tissue lymphoma and diffuse large B-cell lymphoma in the parotid salivary gland led us to initially assume a clonal relationship between diffuse large B-cell lymphoma and mucosa-associated lymphoid tissue lymphoma. Epstein-Barr virus was detected by in situ hybridization and polymerase chain reaction in the mucosa-associated lymphoid tissue lymphoma, but not in diffuse large B-cell lymphoma, suggesting that these lymphomas were not clonally related. Fragment analysis of frame region 3 polymerase chain reaction products from microdissected mucosa-associated lymphoid tissue lymphoma and diffuse large B-cell lymphoma components revealed different clonal pattern rearrangements of the immunoglobulin heavy chain gene.

Conclusions
Our patient’s case highlights the importance of assessing the clonal relationships of the lymphoid components of a composite lymphoma and Epstein-Barr virus screening in mucosa-associated lymphoid tissue lymphoma in patients with autoimmune disease.
Novel stability criteria on nonlinear density-dependent mortality Nicholson’s blowflies systems in asymptotically almost periodic environments
Authors: Qian Chaofan , Hu Yuhui 
Published: January 2020

AbstractIn this paper, we consider nonlinear density-dependent mortality Nicholson’s blowflies system involving patch structures and asymptotically almost periodic environments. By developing an approach based on differential inequality techniques coupled with the Lyapunov function method, some criteria are demonstrated to guarantee the global attractivity of the addressed systems. Finally, we give a numerical example to illustrate the effectiveness and feasibility of the obtain results.
Non-locked and locked small fragment straight plates have a similar behavior in buttressing the posteromedial shear tibial plateau fragment: a biomechanical analysis of three different fixations
Authors: Giordano Vincenzo , Kfuri Mauricio , Belangero William , Venturini Allison , Silva Ana Carolina , Soares Eduardo Merjan , Pires Robinson Esteves , Koch Hilton A. 
Published: January 2020

AbstractPurposeThe aim of this study is to compare the biomechanical behavior of three different fixation constructions currently used for buttressing the posteromedial shearing tibial plateau fragment. Our hypothesis is that non-locked implants provide sufficient comparable stability in posteromedial tibial plateau fractures as locked implants.MethodsFifteen left synthetic tibiae from a single manufacturing batch were used to create a posteromedial shear tibial plateau fracture. The fracture was buttressed with three different posteriorly placed five-hole straight small-fragment plate. Five models were fixed with a one-third tubular plate (TTP), five models with a dynamic compression plate (DCP), and five models with a locking compression plate (LCP). All groups were tested to vertical subsidence (Stage 1). In the same experiment (Stage 2), TTP and DCP groups were tested until catastrophic failure. Force versus displacement curves were obtained in the two stages of the experiment.ResultsStage 1 – There was no significant difference in stiffness (p = 0.89), subsidence up to 2 mm (p = 0.38), and energy (p = 0.36) among the three fixation constructions. Stage 2 – Yield load revealed significantly less yield strength for the TTP group as compared with the DCP group (p = 0.048). However, there was no significant difference in maximum load to failure among the TTP and DCP fixation constructions (p = 0.16).ConclusionPlacement of either a locked or non-locked small fragment straight plate to buttress the posteromedial shear tibial plateau fragment has a similar biomechanical behavior. When the implant is positioned to buttress the shearing fragment it maximizes biomechanical stiffness.
Microbial community dynamics and coexistence in a sulfide-driven phototrophic bloom
Authors: Bhatnagar Srijak , Cowley Elise S. , Kopf Sebastian H. , Pérez Castro Sherlynette , Kearney Sean , Dawson Scott C. , Hanselmann Kurt , Ruff S. Emil 
Published: January 2020

Abstract
Background
Lagoons are common along coastlines worldwide and are important for biogeochemical element cycling, coastal biodiversity, coastal erosion protection and blue carbon sequestration. These ecosystems are frequently disturbed by weather, tides, and human activities. Here, we investigated a shallow lagoon in New England. The brackish ecosystem releases hydrogen sulfide particularly upon physical disturbance, causing blooms of anoxygenic sulfur-oxidizing phototrophs. To study the habitat, microbial community structure, assembly and function we carried out in situ experiments investigating the bloom dynamics over time.

Results
Phototrophic microbial mats and permanently or seasonally stratified water columns commonly contain multiple phototrophic lineages that coexist based on their light, oxygen and nutrient preferences. We describe similar coexistence patterns and ecological niches in estuarine planktonic blooms of phototrophs. The water column showed steep gradients of oxygen, pH, sulfate, sulfide, and salinity. The upper part of the bloom was dominated by aerobic phototrophic Cyanobacteria, the middle and lower parts by anoxygenic purple sulfur bacteria (Chromatiales) and green sulfur bacteria (Chlorobiales), respectively. We show stable coexistence of phototrophic lineages from five bacterial phyla and present metagenome-assembled genomes (MAGs) of two uncultured Chlorobaculum and Prosthecochloris species. In addition to genes involved in sulfur oxidation and photopigment biosynthesis the MAGs contained complete operons encoding for terminal oxidases. The metagenomes also contained numerous contigs affiliating with Myoviridae viruses, potentially affecting Chlorobi. Our data suggest a short sulfur cycle within the bloom in which elemental sulfur produced by sulfide-oxidizing phototrophs is most likely reduced back to sulfide by Desulfuromonas sp.

Conclusions
The release of sulfide creates a habitat selecting for anoxygenic sulfur-oxidizing phototrophs, which in turn create a niche for sulfur reducers. Strong syntrophism between these guilds apparently drives a short sulfur cycle that may explain the rapid development of the bloom. The fast growth and high biomass yield of Chlorobi-affiliated organisms implies that the studied lineages of green sulfur bacteria can thrive in hypoxic habitats. This oxygen tolerance is corroborated by oxidases found in MAGs of uncultured Chlorobi. The findings improve our understanding of the ecology and ecophysiology of anoxygenic phototrophs and their impact on the coupled biogeochemical cycles of sulfur and carbon.
Thyroid cancer associated with Hashimoto thyroiditis: similarities and differences in an endemic area
Authors: Hussein Osama , Abdelwahab Khaled , Hamdy Omar , Awny Shadi , Megahed Nermin A. , Hafez Mohamed T. , Elalfi Amr F. , Abdelaziz Mahmoud , Gaballah Khaled , Abdelkhalek Mohamed 
Published: January 2020

Abstract
Background
Hashimoto thyroiditis (HT) is an autoimmune lymphocytic thyroiditis and is the most common form of thyroid inflammatory diseases. The association of HT with papillary thyroid carcinoma (PTC) has been described. PTC is the most common form of malignancy associated with HT. When papillary carcinoma develops on top of Hashimoto thyroiditis, the disease tends to be less aggressive and lymph node and extra-thyroidal invasion are infrequent.

Results
We retrospectively examined the pathological features of our patients who were diagnosed with concomitant HT and thyroid cancer. In Egyptian patients, PTC was the main type of malignancy associated with HT (96.2%) and was often multifocal (46.2%). In contrast to the published literature, lymph node invasion and extra-thyroidal extension were as frequent in association with HT as in other cancer cohorts. We also observed the frequent occurrence of Hürthle cell metaplasia (23.1%) and the appreciable incidence of aggressive histological types of PTC (32%).

Conclusion
Thyroid carcinoma with HT may have some aggressive features in areas with endemic goiter background.
Ultrasound Lexicon in diagnosis and management of breast fibroadenoma: when to follow up and when to biopsy
Authors: Ali Engy Adel , Talaat Soha 
Published: January 2020

Abstract
Background
To detect the accuracy of breast ultrasound in diagnosis of fibroadenoma and differentiate between typical and atypical ones. The impact of ultrasound criteria on patient management. (Biopsy versus follow up). A prospective study were done to 71 patients. Diagnosis was reached either by performing follow up study or after revision of core biopsy/surgical specimens.

Results
In our study, we had 35 ultrasound detected atypical fibroadenoma, seven out of the 35 (20 %) proven to be complex fibroadenoma by pathology while in another 20 patients, 36 fibroadenomas with typical criteria were detected by both ultrasound and regular follow up. The most ultrasound features which showed statistical significance were the posterior shadowing feature (100% atypical versus 25% typical with significant p value < 0.01), and vessel arrangement (85.7% versus 0% have central arrangement with significant p value < 0.01) helped to differentiate between simple and complex ones. We calculate the sensitivity, specificity, PPV, and NPV to be 100%, 74%, 46%, and 100%.

Conclusion
Arrangement of vessels and posterior features are the most important criteria to differentiate simple and complex FAD.
Diagnostic value of spinal ultrasound compared to MRI for diagnosis of spinal anomalies in pediatrics
Authors: Tawfik Nermeen A. , Ahmed Ahmed Tohamy , El-Shafei Tarek E. , Habba Mohamed R. 
Published: January 2020

Abstract
Background
Spinal dysraphism (SD) is a spectrum of congenital disorders. MRI is the gold standard technique for diagnosis of SD. Spinal ultrasonography (USG) can be used as a screening tool for SD in infants. The purpose of our study is to assess the diagnostic value of spinal USG in the diagnosis of spinal dysraphism in pediatrics compared to MRI as a gold standard technique. Our prospective, cross-sectional study involved 45 infants and children with suspected spinal dysraphism. All patients were subjected to supervision of their medical history, full neurological examination, spinal ultrasonography and anatomical spine assessment by conventional MRI examination including sagittal and axial T1W & T2W, sagittal & coronal STIR imaging. Comparison of spinal USG with MRI findings was done.

Results
There is excellent overall agreement between MRI and ultrasound diagnosis in the evaluation of spinal cord morphology and bony elements in patients aged ≤ 2 years old (κ = 0.96 and 0.98) respectively, and fair overall agreement between MRI and ultrasound diagnosis (κ = 0.58) in patients aged > 2 years old. The accuracy of spinal USG in diagnosis of spinal dysraphism in patients aged ≤ 2 years old was as the following: specificity (94.5–100%), sensitivity (84.3100%), PPV (86.7–100%) and NPV (85.7–100%), as compared to MRI.

Conclusion
Spinal ultrasound can be used as a first-line screening investigation for SD; patients with USG abnormalities proceed to MRI. Spinal ultrasound is of no worthy value after the age of bone ossification, and MRI is the best modality of diagnosis in older pediatrics.