bluuebunny/crossref_metadata_2025_split huggingface dataset:
Escape boxes: Bringing escape room experience into the classroom
Authors: Veldkamp Alice , Daemen Joke , Teekens Stijn , Koelewijn Stefan , Knippels Marie‐Christine P. J. , van Joolingen Wouter R. 
Published: April 2020

AbstractIn this paper, we present an escape box as a means to introduce the escape room concept into classrooms. Recreational escape rooms have inspired teachers all over the world to adapt the popular entertainment activity for education. Escape rooms are problem‐based and time‐constrained, requiring active and collaborative participants, a setting that teachers want to achieve in their classroom to promote learning. This paper explores the adaptation of the escape room concept into educational escape game boxes. These technology‐enhanced escape boxes have become hybrid learning spaces, merging individual and collaborative learning, as well as physical and digital spaces. The design of the box with assignments on each side puts users face to face with each other and requires them to collaborate in the physical world, instead of being individually absorbed in a digital world. The developed box is a unique concept in the field of escape rooms; the content is adaptable. This paper describes the process leading to the design criteria, the design process, test results and evaluation, and provides recommendations for designing educational escape rooms.
Single‐port robot‐assisted radical prostatectomy: a systematic review and pooled analysis of the preliminary experiences
Authors: Checcucci Enrico , De Cillis Sabrina , Pecoraro Angela , Peretti Dario , Volpi Gabriele , Amparore Daniele , Piramide Federico , Piana Alberto , Manfredi Matteo , Fiori Cristian , Autorino Riccardo , Dasgupta Prokar , Porpiglia Francesco ,   the Uro‐technology and SoMe Working Group of the Young Academic Urologists Working Party of the European Association of urology
Published: April 2020

ObjectiveTo summarize the clinical experiences with single‐port (SP) robot‐assisted radical prostatectomy (RARP) reported in the literature and to describe the peri‐operative and short‐term outcomes of this procedure.Material and MethodsA systematic review of the literature was performed in December 2019 using Medline (via PubMed), Embase (via Ovid), Cochrane databases, Scopus and Web of Science (PROSPERO registry number 164129). All studies that reported intra‐ and peri‐operative data on SP‐RARP were included. Cadaveric series and perineal or partial prostatectomy series were excluded.ResultsThe pooled mean operating time, estimated blood loss, length of hospital stay and catheterization time were 190.55 min, 198.4 mL, 1.86 days and 8.21 days, respectively. The pooled mean number of lymph nodes removed was 8.33, and the pooled rate of positive surgical margins was 33%. The pooled minor complication rate was 15%. Only one urinary leakage and one major complication (transient ischaemic attack) were recorded. Regarding functional outcomes, pooled continence and potency rates at 12 weeks were 55% and 42%, respectively.ConclusionsThe present analysis confirms that SP‐RARP is safe and feasible. This novel robotic platform resulted in similar intra‐operative and peri‐operative outcomes to those obtained with the standard multiport da Vinci system. The advantages of single incision can be translated into a preservation of the patient’s body image and self‐esteem and cosmesis, which have a great impact on a patient's quality of life.
Overexpression of miR‐199/214 is a distinctive feature of iron‐induced and asbestos‐induced sarcomatoid mesothelioma in rats
Authors: Okazaki Yasumasa , Chew Shan Hwu , Nagai Hirotaka , Yamashita Yoriko , Ohara Hiroki , Jiang Li , Akatsuka Shinya , Takahashi Takashi , Toyokuni Shinya 
Published: April 2020

AbstractMalignant mesothelioma (MM) is one of the most lethal tumors in humans. The onset of MM is linked to exposure to asbestos, which generates reactive oxygen species (ROS). ROS are believed to be derived from the frustrated phagocytosis and the iron in asbestos. To explore the pathogenesis of MM, peritoneal MM was induced in rats by the repeated intraperitoneal injection of iron saccharate and nitrilotriacetate. In the present study, we used microarray techniques to screen the microRNA (miR) expression profiles of these MM. We observed that the histological subtype impacted the hierarchical clustering of miR expression profiles and determined that miR‐199/214 is a distinctive feature of iron saccharate‐induced sarcomatoid mesothelioma (SM). Twist1, a transcriptional regulator of the epithelial‐mesenchymal transition, has been shown to activate miR‐199/214 transcription; thus, the expression level of Twist1 was examined in iron‐induced and asbestos‐induced mesotheliomas in rats. Twist1 was exclusively expressed in iron saccharate‐induced SM but not in the epithelioid subtype. The Twist1‐miR‐199/214 axis is activated in iron saccharate‐induced and asbestos‐induced SM. The expression levels of miR‐214 and Twist1 were correlated in an asbestos‐induced MM cell line, suggesting that the Twist1‐miR‐199/214 axis is preserved. MeT5A, an immortalized human mesothelial cell line, was used for the functional analysis of miR. The overexpression of miR‐199/214 promoted cellular proliferation, mobility and phosphorylation of Akt and ERK in MeT5A cells. These results indicate that miR‐199/214 may affect the aggressive biological behavior of SM.
Corrected human papillomavirus vaccination rates for each birth fiscal year in Japan
Authors: Nakagawa Satoshi , Ueda Yutaka , Yagi Asami , Ikeda Sayaka , Hiramatsu Kosuke , Kimura Tadashi 
Published: April 2020

AbstractIn Japan, the serious adverse events after human papillomavirus (HPV) vaccination were widely reported in the media. The Ministry of Health, Labour and Welfare of Japan (MHLW) announced the suspension of the governmental recommendation of HPV vaccine in 2013, and the inoculation rate has since sharply declined. The estimated inoculation rate for each birth fiscal year (FY) announced by the MHLW and the actual numbers for each birth FY surveyed by local governments were very different. In particular, the cumulative vaccination rate of girls born in FY2000 was regarded to be as high as 42.9% by the Council of the MHLW. However, this estimation included a confusion. When the suspension of the governmental recommendation was announced in FY2013, the girls born in FY2000 turned 13 years old, the targeted starting age of the HPV vaccination. The vaccination rate of this generation is considered to be quite low. The numbers were recalculated in this study. This study revealed that the real vaccination rate is only 14.3%. Female individuals born in or after FY2000 have been confirmed to be exposed to the same cervical cancer risk as before the HPV vaccine was introduced in Japan.
Acquired resistance to trastuzumab/pertuzumab or to T‐DM1 in vivo can be overcome by HER2 kinase inhibition with TAS0728
Authors: Irie Hiroki , Kawabata Rumi , Fujioka Yayoi , Nakagawa Fumio , Itadani Hiraku , Nagase Hideki , Ito Kimihiro , Uchida Junji , Ohkubo Shuichi , Matsuo Kenichi 
Published: April 2020

AbstractHER2‐targeting antibodies (trastuzumab, pertuzumab) and a HER2‐directed antibody‐drug conjugate (trastuzumab emtansine: T‐DM1) are used for the treatment of HER2‐overexpressing breast cancer. However, these treatments eventually become ineffective due to acquired resistance and there is an urgent need for alternative therapies. TAS0728 is a small‐molecule, irreversible selective HER2 kinase inhibitor. In the present study, we established new in vivo models of cancer resistance by continuous exposure to a combination of trastuzumab and pertuzumab or to T‐DM1 for evaluating the effect of TAS0728 on HER2 antibody‐resistant populations. Treatment with trastuzumab and pertuzumab or with T‐DM1 initially induced tumor regression in NCI‐N87 xenografts. However, tumor regrowth during treatment indicated loss of drug effectiveness. In tumors with acquired resistance to trastuzumab and pertuzumab or to T‐DM1, HER2‐HER3 phosphorylation was retained. Switching to TAS0728 resulted in a significant anti‐tumor effect associated with HER2‐HER3 signal inhibition. No alternative receptor tyrosine kinase activation was observed in these resistant tumors. Furthermore, in a patient‐derived xenograft model derived from breast cancer refractory to both trastuzumab/pertuzumab and T‐DM1, TAS0728 exerted a potent anti‐tumor effect. These results suggest that tumors with acquired resistance to trastuzumab and pertuzumab and to T‐DM1 are still dependent on oncogenic HER2‐HER3 signaling and are vulnerable to HER2 signal inhibition by TAS0728. These results provide a rationale for TAS0728 therapy for breast cancers that are refractory to established anti‐HER2 therapies.
OTU deubiquitinase 5 inhibits the progression of non‐small cell lung cancer via regulating p53 and PDCD5
Authors: Kang Xiao‐yun , Zhang Jing , Tang Ling , Huang Liu , Tong Jin , Fu Qiang 
Published: April 2020

AbstractNon‐small cell lung cancer (NSCLC) has the highest morbidity and mortality worldwide. OTU deubiquitinase 5 (OTUD5), a deubiquitinating enzyme, can enhance the stability of p53 and programmed cell death 5 (PDCD5), a protein related to the apoptosis, by deubiquitination. This study aimed to explore the biological function and underlying mechanism of OTUD5 in NSCLC. Western blot and qRT‐PCR were used to detect the expression of OTUD5 protein and mRNA in NSCLC tissues and cells, respectively. RNAi was adopted to construct an OTUD5 low‐expression model while the plasmids overexpressing p53 and PDCD5 were used to establish the overexpression models, respectively. CCK‐8 assay, transwell assay, and apoptosis assay were carried out to analyze the changes in the proliferation, migration, and chemoresistance of A549 and HCC827 cells. The mechanism of OTUD5 in NSCLC was studied by Western blot. Down‐regulated OTUD5 in NSCLC tissues was significantly correlated to a poor prognosis. The knockdown of OTUD5 inactivated p53 and PDCD5, promoting the proliferation and metastasis of NSCLC cells while inhibiting their apoptosis. OTUD5 knockdown also enhanced the resistance of NSCLC cells to doxorubicin and cisplatin. OTUD5 acted as a tumor suppressor in NSCLC by regulating the p53 and PDCD5 pathways.
The Development of a Scarcity Bias
Authors: Ferera Matar , Benozio Avi , Diesendruck Gil 
Published: April 2020

Adults’ attraction to rare objects has been variously attributed to fundamental biases related to resource availability, self‐related needs, or beliefs about social and market forces. The current three studies investigated the scarcity bias in 11‐ and 14‐month‐old infants, and 3‐ to 6‐year‐old children (N = 129). With slight methodological modifications, participants had to choose between one of 10 same‐kind‐items (abundant resource), or the only one of a different kind (scarce resource). It was found that a robust preference for the scarce resource appeared only at age 5 years. Thus, although a scarcity bias is not present in infancy, it emerges prior to comprehension of market forces. Possible accounts of this developmental finding are discussed.
Differentiated thyroid cancer mortality by disease stage in northern England
Authors: Perros Petros , Mason David , Pearce Mark , Pearce Simon H. S. , Chandler Robert , Mallick Ujjal K. 
Published: April 2020

AbstractObjectiveMortality from thyroid cancer is reported to be higher in the UK compared with several other European countries, though UK data on mortality by disease stage have not been published. The aim of this study was to ascertain disease‐specific mortality by stage in our centre.Design, patients and measurementsThis was a cohort study of all patients presenting to a single centre. Four hundred and twenty patients treated between 2000 and 2010 were identified. The medical records and causes of deaths were reviewed and analysed.ResultsOverall disease‐specific mortality at 5 and 10 years was 1.4% and 5.8%, respectively. The observed mortality was 58 against 66.3 expected deaths (CI 43.8‐75.4) thus yielding an age‐standardized mortality rate of 0.87. There were no deaths due to thyroid cancer in patients with stage I disease at 5 or 10 years. The 10‐year disease‐specific mortality rose with stage (stage II 3.1%, stage III 28.6%, stage IV 30%).ConclusionsThyroid cancer mortality of patients treated at our centre was lower than the official national UK registry and most European figures.
Reconciling care and justice in contesting social harm through performance and arts practice with looked after children and care leavers
Authors: Benaton Tonimarie , Bowers‐Brown Tamsin , Dodsley Thomas , Manning‐Jones Alix , Murden Jade , Nunn Alexander ,   The Plus One Community
Published: April 2020

AbstractThe proportion of young people taken into the care of the state has increased recently and there is evidence that this social group suffer negative long‐term outcomes that might be conceptualised by the emergent criminological category of ‘social harm’. Debates in social work around an ethics of care and justice offer different ways of thinking about responding to social harm. This paper reports findings from an innovative arts‐based intervention with Looked After Children and young people and concludes that holding these competing value sets in creative tension is central to the success of the programme in helping young people to cope with and contest social harm.
Household behavior with respect to meat consumption in the presence of BSE and CWD
Authors: Myae Aye Chan , Goddard Ellen 
Published: April 2020

AbstractIn this study, Canadian household meat consumption behavior in exotic (deer and elk meat) and traditional meats (beef, pork, chicken, turkey, bison, and seafood) is examined. This research introduces some differences in public response to transmissible spongiform encephalopathies (TSE) risks across consumer segments from different sources of meat supply, including hunted meat. The analysis uses a combination of survey and household meat purchase data that include a balanced household panel of 2,393 households per year across time. A two‐step estimation procedure is used with a probit model in the first step (consume or not) and a doublelog–translog two‐stage demand system in the second step (level of consumption). It is assumed and tested that household sociodemographics, consumers’ risk perceptions/attitudes, and media coverage of bovine spongiform encephalopathy (BSE) and chronic wasting disease (CWD) are underlying demand shifters in consumers’ daily meat purchase decision. The results suggest that households who obtain venison from hunting show the highest confidence in venison safety. Exotic meat preferences negatively affect traditional meat consumption in the daily diets of households who purchase traditional meats from stores and obtain venison from hunting. In response to the media coverage of BSE and CWD, exotic meat eaters are less sensitive to animal disease media information than the general population.
Indium and iridium: Two rare metals with a high rate of contact sensitization
Authors: Terrani Isabella , Scherer Hofmeier Kathrin , Bircher Andreas J. 
Published: April 2020

AbstractBackgroundHumans are exposed to a variety of metals on a daily basis, and nickel is the most frequent contact allergen. Little is known about the frequency of sensitization to indium and iridium.ObjectivesStudy the prevalence of indium and iridium sensitization and evaluate the optimal patch test conditions.MethodsA total of 364 patients were patch tested at the allergy unit of the University Hospital of Basel. Pure metals, metal chlorides, and metal sulfates were applied in petrolatum or water in Inert Quadrate (IQ) test chambers for 2 days and read twice at day (D) 2, and between D4 and D7.ResultsEleven patients reacted to indium salts (3.0%), 13 to iridium salts (3.6%), and one reacted to both salts. None of the patients reacted to pure metals. Nineteen of the 23 patients who reacted either to indium or iridium showed concomitant positive reactions to other metals, mainly nickel and palladium.ConclusionThis retrospective clinical study provides insight into the prevalence and test conditions of two rarely tested metal allergens in a large patient cohort. A considerable number of indium‐ or iridium‐positive subjects had co‐sensitization to other metals.
Natural ingredients in cosmetic products—A suggestion for a screening series for skin allergy
Authors: Bruusgaard‐Mouritsen Maria A. , Johansen Jeanne D. , Zachariae Claus , Kirkeby Christel S. , Garvey Lene H. 
Published: April 2020

AbstractBackgroundNaturally derived cosmetic product ingredients of both plant and animal origin are being included increasingly in product formulations in order to cater to consumer preferences. They may be an overlooked cause of reactions to cosmetic products in some patients with dermatitis.ObjectivesTo identify naturally derived cosmetic product ingredients with allergenic potential (type I and type IV) and propose a cosmetic screening test series.MethodsThe study was conducted in two steps. The first step was a market survey using a nonprofit application helping consumers avoid problematic substances in cosmetic products. The application contained 10 067 cosmetic products that were label checked for naturally derived cosmetic product ingredients. The second step was a literature search to examine how frequently the naturally derived ingredients were described and related to allergic reactions in cosmetics or other topically administered products.ResultsWe identified 121 different naturally derived cosmetic product ingredients that were included in at least 30 cosmetic products. In total, 22 ingredients were selected for a screening test series.ConclusionsWe propose a supplemental patch test and a prick test screening series with naturally derived cosmetic product ingredients for patients with skin reactions to cosmetic products, aiming to identify a cause in more patients than is currently possible.
Clinical relevance of endoscopy with histopathological assessment in children with suspected gastrointestinal graft‐versus‐host disease
Authors: Mårtensson Thomas , Mellgren Karin , Toporski Jacek , Arvidson Johan , Szakos Attila , Casswall Thomas H. , Gustafsson Britt 
Published: April 2020

AbstractEndoscopy with histopathological assessment is an established practice to confirm gastrointestinal graft‐versus‐host disease (GI‐GVHD). However, the clinical relevance of this approach in children is incompletely evaluated. In a retrospective cohort study, we investigated the frequency of treatment changes in response to histopathological findings in all children (<18 years) in Sweden who underwent endoscopy for suspected GI‐GVHD (2000‐2013) after receiving hematopoietic stem cell transplantation. Sixty‐eight children with ninety‐one endoscopic occasions were enrolled. At the time of endoscopy, anti‐GI‐GVHD treatment was ongoing in 71% (65/91). In 18% (12/65) with ongoing treatment, no histopathological evidence of GI‐GVHD or another cause to justify anti‐GI‐GVHD treatment was found. In 48% (44/91), endoscopy with histopathological assessment led to changes in the treatment regimen. Re‐endoscopy was more frequent among those with treatment changes, versus unchanged treatment, 39% (17/44) and 13% (6/47), respectively (P = .007). Histopathological findings generating treatment changes were as follows: GI‐GVHD in 68% (30/44), normal histology in 25% (11/44), and an alternative diagnosis in 7% (3/44). In conclusion, this study supports that endoscopy with histopathological assessment should be considered in all children with suspected GI‐GVHD.
Digital mucous cyst: Altered epidermal mucin as a clue to diagnosis
Authors: Moradi Sara , Ricci Andrew , Ehrig Torsten 
Published: April 2020

AbstractBackgroundDigital mucous cyst (DMC) is histopathologically characterized by accumulation of mucin in the dermis. Some cases of DMC also show epidermal mucin, the histopathologic appearance and staining properties of which have not been described in detail.MethodsA total of 24 cases of DMC were investigated by routine hematoxylin‐eosin (H&E) and Alcian blue stains in addition to AE1/AE3 immunohistochemistry.ResultsNine out of the 24 cases of DMC showed epidermal mucin. As the epidermal mucin migrates upward within the epidermis, it transforms from a flocculent granular substance into one or several solid horizontal plugs with a more homogeneous appearance and incorporates cytoplasmic fragments of keratinocytes/corneocytes. The homogeneous mucin plugs stain eosinophilic or amphophilic with an H&E formulation using hematoxylin 7212 and basophilic with Gill 3 or Harris's hematoxylin. The eosinophilic staining is enhanced when the eosin solution contains phloxine.ConclusionsThe variably eosinophilic, amphophilic, or basophilic staining of epidermal mucin can be explained by its composition of basophilic mucin and eosinophilic debris from cytoplasmic fragments. The eosinophilic staining of mucin has not been reported before and can be diagnostically important because it may be mistaken for serum exudate.
Impact of image analysis and artificial intelligence in thyroid pathology, with particular reference to cytological aspects
Authors: Girolami Ilaria , Marletta Stefano , Pantanowitz Liron , Torresani Evelin , Ghimenton Claudio , Barbareschi Mattia , Scarpa Aldo , Brunelli Matteo , Barresi Valeria , Trimboli Pierpaolo , Eccher Albino 
Published: April 2020

ObjectiveThyroid pathology has great potential for automated/artificial intelligence algorithm application as the incidence of thyroid nodules is increasing and the indeterminate interpretation rate of fine‐needle aspiration remains relatively high. The aim of the study is to review the published literature on automated image analysis and artificial intelligence applications to thyroid pathology with whole‐slide imaging.MethodsSystematic search was carried out in electronic databases. Studies dealing with thyroid pathology and use of automated algorithms applied to whole‐slide imaging were included. Quality of studies was assessed with a modified QUADAS‐2 tool.ResultsOf 919 retrieved articles, 19 were included. The main themes addressed were the comparison of automated assessment of immunohistochemical staining with manual pathologist's assessment, quantification of differences in cellular and nuclear parameters among tumour entities, and discrimination between benign and malignant nodules. Correlation coefficients with manual assessment were higher than 0.76 and diagnostic performance of automated models was comparable with an expert pathologist diagnosis. Computational difficulties were related to the large size of whole‐slide images.ConclusionsOverall, the results are promising and it is likely that, with the resolution of technical issues, the application of automated algorithms in thyroid pathology will increase and be adopted following suitable validation studies.