bluuebunny/crossref_metadata_2025_split huggingface dataset:
WITHDRAWN: Reuse of Waste Materials as Precursors of Activated Carbon Synthesis for the Abatement of Antibiotic Cefpodoxime Proxetil from Industrial Effluents.
Authors: Manzoor Suryyia , Abbas Naseem , Fernandez Javier , Mirza Amir , Yasmin Ghazala , Khan Muhammad Imran , Rao Zunaira , Rizwan Muhammad , Waqar Muhammad , Majeed Abdul 
Published: November 2020

Abstract
The release of antibiotics to aquatic environment creates aquatic ecotoxicity and their bioaccumulation results in antibiotic resistance. Hence to lessen the risk of ecotoxicity and depletion of natural resources, it is imperative to disclose alternate precursor raw materials that can be successfully employed for the synthesis of new sorbents capable to remove antibiotics from the environment. This research reports the synthesis of activated carbon based sorbents derived from waste biomaterials like coconut shell (CNAC), walnut shell & peach stone (WNAC) and Bombax ceiba fruit capsule (BCAC) and their ability to remove antibiotic Cefpodoxime from the industrial effluents. Activation of carbon was done by HNO3 and H3PO4. FTIR, BET and SEM techniques were used for characterization of sorbents. Langmuir and Freundlich isotherm models were applied to study the adsorption behavior. Moreover, the experimental adsorption capacity (qe) of the three activated carbons was found to be 32 mg/g for CNAC, 40 mg/g for WNAC and 10 mg/g for BCAC. The maximum removal efficiency of the drug was found to be 96% using the WNAC. HPLC analysis was performed to confirm the removal of Cefpodoxime from industrial effluent.
miR-141 and miR-200a are involved in Th17 cell differentiation by negatively regulating RARB expression
Authors: Bahmani Leila , Baghi Masoud , Peymani Maryam , Javeri Arash , Ghaedi Kamran 
Published: November 2020

Abstract
Background: Among T helper (Th) lineages differentiated from naïve CD4+ T cells, interleukin (IL)-17-producing Th17 cells are highly correlated with the pathogenesis of autoimmune disorders. Although a series of microRNAs (miRs) that modulate autoimmunity progress have been reported, further studies on microRNAs particularly involved in Th17 lineage commitment are still warranted. Results: In this study, to clarify the involvement of miR-141-3p and miR-200a-3p in Th17 cell differentiation, as well as to explore their potential target gene involved, purified human naïve CD4+ T cells were cultured under Th17 cell polarizing condition. Differentiation process was confirmed applying ELISA method to measure IL-17 secretion and RT-qPCR to assess Th17 cell-defining genes expression during differentiation period. miR-141 and miR-200a expression pattern as well as expression alteration of their predicted downstream genes, which were identified via consensus and integration in-silico approach, was then evaluated by RT-qPCR. Finally direct interaction between both microRNAs and their common predicted target sequence was approved by dual-luciferase reporter assay. Highly increased IL-17 secretion and Th17 lineage-specific genes expression confirmed Th17 cell differentiation. Conclusions: Results demonstrated that miR-141 and miR-200a were Th17 cell-associated microRNAs and their expression levels upregulated significantly during Th17 cell induction. We also found that retinoic acid receptor beta (RARB) gene, whose product has been reported as a negative regulator of Th17 cell generation, was a direct target of both microRNAs and its downregulation could affect the transcriptional level of JAK/STAT pathway genes. Overall, our results introduced two novel Th17 lineage-associated microRNAs and provided a rationale to further clarify the RARB-dependent mechanism of miR-141 and miR-200a to promote Th17 cell differentiation and hence Th17-mediated autoimmunity.
The Lipid-related Effects of Resveratrol on Human Ectopic Endometrial Stromal Cells and a Rat Model of Endometriosis
Authors: Chen Zhengyun , Wang Chunyan , Lin Cuicui , Zhang Lifeng , Zheng Huimei , Zhou Yong , Li Xiaoyong , Li Chen , Zhang Xinmei , Yang Xiaohang , Guan Minxin , Xi Yongmei 
Published: November 2020

Abstract
Background: Endometriosis is a complex disease in the field of gynecology that has certain limitations for its interim treatment. Resveratrol has been recently used for the treatment of endometriosis in experimental and clinical studies, but its molecular mechanism remaines elusive. Results: In this study, based on a case-control study, we identified that a decreased BMI and altered lipid profiles were associated with endometriosis patients. We applied resveratrol treatment on human ectopic endometrial stromal cells (HEcESCs) and a rat model of endometriosis. Lipidomics analysis showed that resveratrol altered lipid profiles in HEcESCs, with the sphingolipids Cer and SM increased significantly, while FA and most phospholipids were significantly reduced. Pathway enrichment analysis showed that several lipid-associated signaling pathways could be targeted by resveratrol. Our experiments in a rat model showed that resveratrol reduced the lesion and rectified lipid profiles in rats with endometriosis. In addition, resveratrol treatment significantly increased the expression of PPARα in lesion tissues of model rats and HEcESCs of EMs patients.Conclusion: Our data reveal that the development of EMs is closely related to lipid metabolism, and resveratrol may play a therapeutic role by targeting the lipid metabolism of ectopic endometrial stromal cells in endometrosis. Our study provides valuable insights for understanding the pathogenesis and clinical treatment of endometriosis.
The Prevalence and Characteristic of Sleep Problems among Children with Fetal Alcohol Spectrum Disorders (FASD).
Authors: Dyląg Katarzyna Anna , Bańdo Bożena , Baran Zbigniew , Dumnicka Paulina , Kowalska Katarzyna , Kułaga Paulina , Przybyszewska Katarzyna , Radliński Jakub , Roozen Sylvia , Curfs Leopold 
Published: November 2020

Abstract
BackgroundFetal alcohol spectrum disorders (FASD) is a group of conditions resulting from prenatal alcohol exposure (PAE). Patients with FASD experience a variety of neuropsychological symptoms resulting from central nervous system impairment. Little is known about sleep disorders associated with PAE. The objective of this study was to investigate the prevalence and characterize sleep problems among individuals with FASD.Methods40 patients from FASD diagnostic center (median age 8 years (6; 11)) diagnosed with one of the FASD diagnosis and 40 patients from the control group (median age 10 years (8; 13)) participated in the study. In the 1st phase, the screening of sleep problems was performed with Child Sleep Habit Questionnaire (CSHQ) filled in by a caregiver. Those who scored above 41 points were qualified to the 2nd phase of the study in which in-lab attended polysomnography (PSG) was performed. The measurements consisted of electroencephalographic channels, left and right electrooculograms, chin electromyogram, left and right tibialis electromyogram, electrocardiogram, ventilatory monitoring, breathing effort, pulse oximetry, snoring and body position.ResultsThe number of participants with sleep disturbances was markedly higher in the study group as compared to controls (55% vs. 20%). The age-adjusted odds ratio for a positive result in CSHQ was 4.31 (95% CI: 1.54-12.11; p=0.005) for FASD patients as compared to the control group. Significant differences between the study and control groups were observed in the following subscales: sleep onset delay, night wakings , parasomnias , sleep disordered breathing, and daytime sleepiness. In PSG children from the FASD group and children from the control group experienced more arousals during the sleep than the children from the laboratory control group. ). There were no significant differences in comparison of respiratory indexes between FASD and control groups although indices in both groups were higher than previously published data for a normal population.ConclusionSleep disorders are more common among individuals with FASD than among healthy children. As distorted sleep can affect daily performance and worsen neurodevelopmental issues, it is vital for the pediatricians and other FASD specialists to include the question about sleep in the anamnesis.
Unusual presentation of a medulloblastoma mimicking an AARS (Atlanto axial rotatory subluxation). A case report.
Authors: Greggi Tiziana , Boriani Luca , Scarale Antonio , Martikos Konstantinos , Vommaro Francesco , Leggi Lucrezia , Ciani Giovanni , Battaglia Milva , Ghizzi Chiara , Zucchelli Mino 
Published: November 2020

Abstract
Background: Atlanto-Axial Rotatory Subluxation is an acquired condition, usually occurring in pediatric patients, because of either trauma or pharyngitis or ear-nose-throat ( ENT) surgery or upper respiratory tract infection. Post-traumatic C1-C2 rotational subluxation in pediatric age is a very frequent event. It mimics benign torticollis but may result in permanent disability. The atlanto-axial rotatory displacement, as defined initially by Fielding and Hawkins in 1977, is classified into four types: Type 1 is the most common form in children. It is a simple rotatory displacement without an anterior shift. Type 2 is potentially more dangerous. It is a rotatory displacement with an anterior shift of 5 mm or less. Type 3 is rotatory displacement with an anterior shift greater than 5 mm. Finally, type 4 is rotatory displacement with a posterior shift. Type 3 and 4 deformities are rare, but neurological involvement or even instant death may follow [Fig. 4] (18). The condition requires prompt diagnosis by thorough examination to avoid any treatment delays. The difficulties for a timely and correct diagnosis and then the correct treatment are described. Case presentation: A 5 year old girl comes to the emergency room with cervical pain and stiffness.This painful state, as mum reports, has been lasted for about ten days, after a mild trauma. The radiographic picture demonstrates a rotary subluxation C1-C2, type 1 according to Fielding and Hawkins classificationThe treatment is head-halter traction with 2kg, and nonsteroidal anti-inflammatory drugs (NSAIDs) . After 2 days the x-ray picture improves, but the painful symptoms persist. An in-depth diagnostic MRI of the cervical spine and brain revealed a medulloblastoma which made an immediate and effective neurosurgical intervention necessary. Conclusions: the C1-C2 rotatory dislocation must always be evaluated with extreme care, without neglecting any clinical and anamnestic information for a correct and timely differential diagnosis. Remember that children show very clearly what they feel, always .
Developing A New One-Dimensional Scale To Measure Pain in Children: Pencil Pain Scale
Authors: BEYAZ Emine , TÜFEKCİ Fatma GÜDÜCÜ 
Published: November 2020

Abstract
Background: The aim of this study was to develop the Pencil Pain Scale as a new one-dimensional scale for evaluating pain in school-age children (6-12 years old).Methods: The population of this study is composed of 6-12 year-old children who gave blood for examination rather than for an acute or severe cause. The research sample included randomly selected children (N= 464) who applied to the blood collection units of a state hospital between November 2017 - February 2018, on three days of the week (Monday, Wednesday, Friday).A questionnaire that specifies the characteristics of children, the newly developed Pencil Pain Scale, and the two widely used scales (Visual Analogue Scale and the Facial Expressions Rating Scale) were used to collect data. The data obtained were analyzed with percentage distributions, mean, chi-square, t-test, ANOVA, Kruskal Wallis, Mann Whitney U test and correlation. Ethical principles were followed in the study.Results: According to expert opinions, it was determined that the Pencil Pain Scale had content validity (Content Validity Index= +1.00). It was found that the scale made measurements similar to a previously conducted study and had convergent validity. Also, the scale was found to be sensitive enough to distinguish the differences and to be reliable (p<.001). It was also found that the Pencil Pain Scale performs measurements that are in agreement with the Visual Analog Scale and the Facial Expression Rating Scale and has parallel form reliability (p>.05). The Pencil Pain Scale was developed in a valid and reliable way. Conclusion: The Pencil Pain Scale is a valid and reliable scale. In order to make the Pencil Pain Scale a standard scale for measuring children's pain, it needs to be used in different studies and age groups with different scales for different pain types.
Therapeutic monitoring of the host-bacterium-phage interaction in a child
Authors: Khatami Ameneh , Lin Ruby , Petrovic-Fabijan Aleksandra , Alkalay-Oren Sivan , Almuzam Sulaiman , Britton Philip , Brownstein Michael , Dao Quang , Fackler Joseph , Hazan Ronen , Horne Bri’Anna , Nir-Paz Ran , Iredell Jonathan 
Published: November 2020

Abstract
Successful phage therapy for extensive chronic osteoarticular infection in a child resulted in an initial flush of bacterial contents into the bloodstream with an inflammatory response marked by fever, local pain and upregulation of genes associated with autophagy and innate immunity. Monitoring of phage and bacterial kinetics and the human host response allowed early dose adjustment and indicated a strong therapeutic effect within two weeks.
Metabolic profiling of neocortical tissue discriminates Alzheimer’s disease from mild cognitive impairment, high pathology controls, and normal controls
Authors: Jasbi Paniz , Shi Xiaojian , Chu Ping , Elliott Natalie , Hudson Haley , Jones Douglas , Serrano Geidy , Beach Thomas , Liu Li , Jentarra Garilyn , Gu Haiwei 
Published: November 2020

Abstract
Background

Alzheimer’s disease (AD) is the most common cause of dementia, accounting for an estimated 60 to 80% of cases, and is the sixth-leading cause of death in the United States. While considerable advancements have been made in the clinical care of AD, it remains a complicated disorder that can be difficult to identify definitively in its earliest stages. Recently, mass spectrometry (MS)-based metabolomics has shown significant potential for elucidation of disease mechanisms and identification of therapeutic targets as well diagnostic and prognostic markers that may be useful in resolving some of the difficulties affecting clinical AD studies, such as effective stratification.
Methods

In this study, complementary gas chromatography- and liquid chromatography-MS platforms were used to detect and monitor 2,080 metabolites and features in 48 post-mortem tissue samples harvested from the superior frontal gyrus of male and female subjects. Samples were taken from four groups: 12 normal control (NC) patients, 12 cognitively normal subjects characterized as high pathology controls (HPC), 12 subjects with non-specific mild cognitive impairment (MCI), and 12 subjects with AD.
Results

Multivariate statistics informed the construction and cross-validation (p < 0.01) of partial least squares-discriminant analysis (PLS-DA) models defined by a 9-metabolite panel of disease markers (lauric acid, stearic acid, myristic acid, palmitic acid, palmitoleic acid, and four unidentified mass spectral features). Receiver operating characteristic analysis showed high predictive accuracy of the resulting PLS-DA models for discrimination of NC (97%), HPC (92%), MCI (~ 96%), and AD (~ 96%) groups. Pathway analysis revealed significant disturbances in lysine degradation, fatty acid metabolism, and the degradation of branched-chain amino acids. Network analysis showed significant enrichment of 11 enzymes, predominantly within the mitochondria.
Conclusions

The results expand basic knowledge of the metabolome related to AD and reveal pathways that can be targeted therapeutically. This study also provides a promising basis for the development of larger multi-site projects to validate these candidate markers in readily available biospecimens such as blood to enable the effective screening, rapid diagnosis, accurate surveillance, and therapeutic monitoring of AD.
Plasma Metabolomic Profiles and Clinical Features in Recovered COVID-19 Patients Without Previous Underlying Diseases 3 Months After Discharge
Authors: Wang Sufei , Xu Juanjuan , Luo Ping , Yang Lian , Song Siwei , Tan Xueyun , Chen Qing , Xia Hui , Zhang Shujing , Ma Pei , Ma Yanling , Yang Fan , Liu Yu , Li Yumei , Ma Guanzhou , Wang Zhihui , Duan Yanran , Jin Yang 
Published: November 2020

Abstract
Knowing the residual and future effect of SARS-CoV-2 on recovered COVID-19 patients is critical for optimized long-term patient management. Recent studies focus on the symptoms and clinical indices of recovered patients, but the pathophysiological change is still unclear. To address this question, we examined the metabolomic profiles of recovered asymptomatic (RA), moderate (RM) and severe and critical (RC) patients without previous underlying diseases discharged from the hospital for 3 months, along with laboratory and CT findings. We found that the serum metabolic profiles in recovered COVID-19 patients still conspicuously differed from that in healthy control (HC), especially in the RM, and RC patients. Additionally, these changes bore close relationship with the function of pulmonary, renal, hepatic, microbial and energetic metabolism and inflammation. These findings suggested that RM and RC patients sustained multi-organ and multi-system damage and these patients should be followed up on regular basis for possible organ and system damage.
Hyperbaric Oxygen Enhanced Mitochondria-Targeted Chemotherapy in Bladder Cancer
Authors: Shen Chongxing , Yue Xiaofeng , Dai Linyong , Wang Jianwu , Li Jinjin , Fang Qiang , Zhi Yi , Shi Chunmeng , Li weibing 
Published: November 2020

Abstract
Background: Bladder cancer has a high rate of recurrence and drug resistance due to a lack of effective therapies. IR-780 iodide, a near-infrared (NIR) mitochondria-targeting fluorescent agent, has been demonstrated to achieve higher selectivity than other drugs in different tumor types. In the study, we aimed to investigate the anti-tumor effect of IR-780 combined with hyperbaric oxygen (HBO) on bladder cancer.Methods: Using in vitro cell line data, in vivo model data and clinical data, we tested the ability of IR-780 to selectively accumulate in bladder cancer. We also evaluated the anti-tumor effect of IR-780 combined or not with HBO both in vitro and in vivo, and explored the potential mechanism of its anti-tumor effect. Results: We revealed for the first time that IR-780 selectively accumulated in bladder cancer (bladder cancer cells, xenografts and bladder cancer samples from patients) and could induce cancer cell apoptosis by targeting the mitochondrial complex I protein NDUFS1. Further study displayed that the combination with HBO could significantly enhance the antitumor effect of IR-780 in vitro by promoting cancer cell uptake and inducing excessive mitochondrial reactive oxygen species (ROS) production, while suppressing tumor growth and recurrence in animal models without causing apparent toxicity. Moreover, this combination antitumor strategy was also demonstrated in drug-resistant bladder cancer cells (T24/DDP) and xenografts. Conclusions: These data identify for the first time a combination of IR-780 and HBO (IR-780+HBO), which exhibits mitochondria-targeting and therapeutic capabilities, as a novel treatment paradigm for bladder cancer.
OLFML2A Downregulation Inhibits Glioma Proliferation Through Suppression of Wnt/β-Catenin Signaling
Authors: Ma Shize , Duan Lei , Dong Huateng , Ma Xiaodong , Guo Xinyu , Liu Jianli , Li Guoqiang , Yu Yue , Xu Yanlong , Yuan Guoqiang , Zhao Xingkun , Tian Guopeng , Zhai Shijia , Pan Yawen , Zhang Yinian 
Published: November 2020

Abstract
Background: Glioma is a highly heterogeneous and lethal tumor with extremely poor prognosis. Through analysis of TCGA data, we found that OLFML2A was significantly upregulated in glioma tissues and positively correlated with glioma grade and worse prognosis. However, the molecular function of OLFML2A and its underlying mechanism in glioma remain unclear.Methods: The expression of OLFML2A in glioma was determined by immunohistochemistry (IHC). Celigo assay, MTT assay and flow cytometry were utilized to evaluate the effects of OLFML2A on glioma proliferation and apoptosis. Gene chip microarray analysis and ingenuity pathway analysis were used to investigate the potential regulatory mechanisms of OLFML2A, which were further assessed by q-PCR, western blotting and IHC. An animal transplanting glioma model and spectral computed tomography scan were used to verify OLFML2A expression in vivo.Results: In this study, we found that the expression of OLFML2A was significantly upregulated in glioma specimens and positively correlated with pathological grades in glioma patients. Moreover, Kaplan-Meier survival analysis of TCGA data revealed that glioma patients with higher expression of OLFML2A had shorter overall survival. Importantly, when we knocked down the expression of OLFML2A in glioma cells, cell proliferation was inhibited, and apoptosis was promoted. Mechanistically, downregulation of OLFML2A inhibited Wnt/β-catenin signaling by directly reducing the level of stabilized β-catenin and upregulating the expression of amyloid precursor protein (APP) to indirectly suppress β-catenin, leading to repression of MYC, CD44 and CSKN2A2 expression. Furthermore, we found that OLFML2A downregulation clearly suppressed the growth of subcutaneous glioma and intracranial transplantation of glioma by inhibiting Wnt/β-catenin pathway-dependent cell proliferation.Conclusion: Our data indicate the oncogenic effect of OLFML2A in glioma through regulation of Wnt/β-catenin signaling, which may provide a novel therapeutic target for glioma.