bluuebunny/crossref_metadata_2025_split huggingface dataset:
Aging and metalloproteinases expression in mussels extracellular matrix
Authors: Bosiacki Mateusz , Lubkowska Anna 
Published: January 2020

Abstract
Emerging traits of cachexia in the elderly are usually attributed to disease, reduced physical activity, reduced appetite, reduced metabolic rate, and reduced energy demand. Lower physical activity and malnutrition lead to sarcopenia, understood as age-related skeletal muscle loss, often associated with reductions in strength, speed of contraction, and muscle power. In addition, the loss of muscle mass observed in old age may be due to changes in the nervous and endocrine systems, changes in muscle metabolism, decreased cell proliferation capacity, chronic age-related inflammation and oxidative stress, chronic disease conditions, and use of pharmaceuticals.
Many diseases of muscle are accompanied by increased expression and activity of matrix metalloproteinases (MMP) from the zinc-dependent endopeptidase family, which are involved in the matrix remodeling process that accompanies muscle regeneration. Disorders of collagen accumulation resulting from changes in MMP expression may lead to pathological processes of muscle fibrosis.
With the exception of the membrane-type matrix metalloproteinases (MT-MMP), MMP are synthesized in cells and secreted into the extracellular matrix (ECM). Secretory MMP can aggregate on cell surfaces by binding to MT-MMP, along with molecules responsible for cell adhesion, integrins or proteoglycans. To date, over 20 subtypes of metalloproteinases have been classified, each coded by different genes.
MMP–induced degradation of structural matrix components (such as collagen, laminin, fibronectin) creates space for suitable cells and facilitates cell migration through the matrix. These enzymes are also involved in the regulation of cell activ ity and take part in inflammatory reactions. Many of the peptides released during partial ECM proteolysis may also act as signaling molecules with autocrine or paracrine action. MMPs also participate in cell-cell communication.
Under physiological conditions, MMP are expressed at a very low level in tissues. Induction of expression occurs only in times of ECM reconstruction. Due to their protease activity, these enzymes remain under strict multi-level regulation, i.e. at the level of transcription, post-translational processing, secretion, activation, and degradation. Active MMPs are regulated by tissue inhibitors of metalloproteinases and by numerous cytokines and growth factors.
Extensive dentigerous cyst associated with a maxillary mesiodens in a 55-year-old male – case report and literature review
Authors: Rubinkiewicz Aleksandra , Sikora Maciej , Sielski Marcin , Stąpor Agata , Chlubek Dariusz 
Published: January 2020

Abstract
Supernumerary teeth as mesiodentes in adults are a rare condition. Given that mesiodentes often interfere with the eruption of normal permanent incisors, the majority are diagnosed in teenagers. In some cases, this pathology is associated with the presence of a dentigerous cyst. Imaging techniques play a significant role in the diagnosis of cysts, particularly cone beam computed tomography (CBCT). Once the correct diagnosis is established, appropriate therapeutic measures – usually surgical – should be initiated promptly.
The case presented here is that of a 55-year-old male with an extensive dentigerous cyst arising from a maxillary mesiodens. The decision was made to perform enucleation of the cyst along with the mesiodens inside it. The procedure was performed under general anesthesia. No complications were observed in the postoperative period. The histopathology report confirmed a dentigerous cyst. Radiograph images obtained 10 months post-surgery showed spontaneous bone tissue reconstruction.
Human microbiome as a source of folate modifiable by nutritional and non-nutritional factors
Authors: Gumiela Dorota 
Published: January 2020

Abstract
Hippocrates, in 400 years before our era, indicated that the intestines are central to human health and that “bad digestion is the root of all evil”. The microbiome influences immune status, protects against colonization by pathogenic microorganisms, performs metabolic and trophic functions, and participates in the synthesis of vitamin K, B1, B6, B12, and folic acid. Diet is a key factor in determining its composition. For many microbial strains, p-aminobenzoic acid is an indispensable substrate for the formation of folates. Bifidobacteria (HRB) strains in humans are mainly responsible for the synthesis of folates. Non-HRB strains that have the ability to synthesize folates include B. thermophilum and B. longum ssp. The only hitherto indicated factor that can affect the microbiota’s potential for folate synthesis is metformin, which improves glucose tolerance in people with type II diabetes. Metformin reduces the production of glucose in the liver by inhibiting gluconeogenesis and glycogenolysis, increases the sensitivity of tissues to insulin, and reduces the absorption of glucose. In order to thoroughly understand the ability of the microbiota to synthesize folic acid, it is necessary to conduct studies that include people with varying degrees of exposure to factors that may affect health, such as: poor dietary habits, smoking, low physical activity, diet-related diseases, or taking medications.
Management of pain in outpatients with critical limb ischemia
Authors: Rynio Paweł , Cnotliwy Miłosław , Kazimierczak Arkadiusz , Rakocz Anna , Dembińska Teresa , Tyszkiewicz-Bandur Monika , Gutowski Piotr 
Published: January 2020

Abstract

Introduction: Critical limb ischemia (CLI) is a condition resulting from chronic impaired limb perfusion by arterial blood, which is always accompanied by rest pain. The key objectives of treatment are to prevent high-level amputations and to manage pain. Currently there are no strong recommendations regarding pharmacotherapy for pain in patients with CLI.
The aim of the study was to evaluate analgesics currently used in patients with CLI on an outpatient basis and to assess their effectiveness in pain treatment.

Materials and methods: The study included 88 patients diagnosed with CLI, who declared taking a painkiller in the past 12 h. Patients were asked for the name of the drug. The truth of the declaration was verified by the presence of the drug in the serum of 45 randomly selected patients. All patients rated pain intensity on a numerical scale (NRS) before and after treatment. Their mental state was evaluated by Mini-Mental State Examination (MMSE) test.

Results: The most commonly used analgesics were ketoprofen, ibuprofen and acetaminophen. Pain management was successful in 11.4% of patients. Average pain intensity before taking the drug was 8.34 points (SD 1.27) and 5.85 (SD 1.57) after. In only 11 patients the presence of the declared drug was confirmed in serum. In 22 patients other drugs were found. For patients who reported that analgesic medication was ineffective, MMSE value averaged 22.1 (SD 4.95) and 25.1 (SD 4.25) for the group reporting effective treatment, p = 0.07. Patients reporting ineffectiveness of medications had suffered for an average duration of 8.2 weeks (SD 1.39), while the effective group average 5.2 weeks (SD 5.13), p = 0.06.

Conclusions: Treatment of pain in patients with CLI in an out-patient setting is ineffective because it is based on medications used in the treatment of nociceptive pain and not neuropathic pain. There is an urgent need to improve the treatment of pain in patients with CLI by educating family doctors.
Necrosis and massive perforation of the small intestine in the course of lymphoma: a case report
Authors: Żyluk Andrzej , Jagielski Wojciech , Mazur-Grzesiuk Agnieszka 
Published: January 2020

Abstract
Necrosis and perforation of the small intestine of non-vascular origin are uncommon. Single cases have been described occurring in patients with blood and lymphatic malignant neoplasms. We present the case of necrosis and massive perforation of part of the small intestine in a patient diagnosed with diffuse large B-cell lymphoma (DLBCL), prior to commencement of therapy. At admission, the patient had typical symptoms and signs of a diffused peritonitis. Intraoperatively, faecal content was found in the peritoneal cavity and necrosis with a massive perforation of approximately 10 cm of the terminal ileum. Resection of the involved fragment of intestine was performed, followed by closing of the distal stump of the ileum and end ileostomy. The post-operative course was uneventful and the patient was moved to the haematologic department for further treatment of the lymphoma.
Arts, mental distress, mental health functioning &amp; life satisfaction: fixed-effects analyses of a nationally-representative panel study
Authors: Wang Senhu , Mak Hei Wan , Fancourt Daisy 
Published: January 2020

Abstract
Background. Arts engagement within communities is ubiquitous across cultures globally and previous research has suggested its benefits for mental health and wellbeing. However, it remains unclear whether these benefits are driven by arts engagement itself or by important confounders such as socio-economic status (SES), childhood arts engagement, previous mental health, personality, or self-selection bias. The aim of this study is to use fixed effects models that account for unidentified time-constant confounding measures to examine the longitudinal association between arts (frequency of both arts participation and cultural attendance), mental distress, mental health functioning and life satisfaction.
Methods. Data from 23,660 individuals (with a mean age of 47 years) included in the UK Understanding Society wave 2 (2010-2012) and wave 5 (2013-2015) were analyzed. Aside from controlling for all time-constant variables using the fixed-effects models, we additionally adjusted for time-varying demographic factors (e.g. age and marital status), health behaviors and social support variables.
Results. After controlling for all time-constant variables and identified time-varying confounders, frequent arts participation and cultural attendance were associated with lower levels of mental distress and higher levels of life satisfaction, with arts participation additionally associated with better mental health functioning. Health-related and social time-varying factors were shown to partly but not wholly explain the observed associations.
Conclusion. Arts engagement amongst the population as a whole may help enhance positive mental health and life satisfaction, and protect against mental distress. These results are not explained by any time-constant confounding factors.
Comprehensive gene expression analysis for exploring the association between glucose metabolism and differentiation of thyroid cancer
Authors: Suh Hoon Young , Choi Hongyoon , Paeng Jin Chul , Cheon Gi Jeong , Chung June-Key , Kang Keon Wook 
Published: January 2020

Abstract
Background: The principle of loss of iodine uptake and increased glucose metabolism according to dedifferentiation of thyroid cancer is clinically assessed by imaging. Though these biological properties are widely applied to appropriate iodine therapy, the understanding of the genomic background of this principle is still lacking. We investigated the association between glucose metabolism and differentiation in advanced thyroid cancer as well as papillary thyroid cancer (PTC).
Methods: We used RNA sequencing of 505 patients with PTC obtained from the Cancer Genome Archives and microarray data of poorly-differentiated and anaplastic thyroid cancer (PDTC/ATC). The signatures of GLUT and glycolysis were estimated to assess glucose metabolic profiles. The glucose metabolic profiles were associated with tumor differentiation score (TDS) and BRAFV600E mutation status. In addition, survival analysis of glucose metabolic profiles was performed for predicting recurrence-free survival.
Results: In PTC, the glycolysis signature was positively correlated with TDS, while the GLUT signature was inversely correlated with TDS. These correlations were significantly stronger in the BRAFV600E negative group than the positive group. Meanwhile, both GLUT and glycolysis signatures were negatively correlated with TDS in advanced thyroid cancer. The high glycolysis signature was significantly associated with poor prognosis in PTC in spite of high TDS. The glucose metabolic profiles are intricately associated with tumor differentiation in PTC and PDTC/ATC.
Conclusions: As glycolysis was an independent prognostic marker, we suggest that the glucose metabolism features of thyroid cancer could be another biological progression marker different from differentiation and provide clinical implications for risk stratification.
Root dentine thickness of danger zone in mesial roots of mandibular first molars
Authors: Zhou Guangchao , Leng Diya , Li Mingming , Zhou Yang , Zhang Cuifeng , Sun Chao , Wu Daming 
Published: January 2020

Abstract
Background: Better understanding of the danger zone anatomy in mesial roots (MRs) of mandibular first molars (MFMs) may serve to decrease the risk of mishaps. This study aimed to measure the minimal distal dentine thicknesses of danger zone in MRs of MFMs in a native Chinese population using cone-beam computed tomography (CBCT). Methods: CBCT images of 1792 MFMs from 898 Chinese patients were analyzed. The minimal distal dentine thicknesses of the mesiobuccal (MB) and mesiolingual (ML) canals below the furcation 1, 2, 3, 4, 5 mm were measured. The association between the minimal distal dentine thicknesses and the root lengths, patient’s age and gender, side were assessed. Results: The minimal distal dentine thicknesses of MB and ML canals are located 3∼4 mm below the furcation for both men and women. There are no differences between MB and ML canals, while the minimal distal dentine thicknesses of MB and ML canals were higher in men than women ( P <0.05), except at 1 and 3 mm of ML canals ( P >0.05). The minimal distal dentine thicknesses of MB and ML canals increased with age in both men and women at each location ( P <0.05). The minimum distal dentine thickness at every location were significantly different between long teeth and short teeth both in men and women ( P < 0.05), with short teeth having the smallest mean values. There are no significant differences between two sides ( P >0.05). Conclusions: The minimal distal dentine thicknesses of MRs in MSMs have close correlation with root length, patient’s age and gender.
Development and preliminary validation of a brief Nurses' Perceived Professional Benefit Questionnaire (NPPBQ)
Authors: HU Yanli , HU Jing , Li Liping , ZHAO Bin , Liu Xiaohong , Li Fan 
Published: January 2020

Abstract
Objective Our objective was to develop and psychometrically test a brief Nurses' Perceived Professional Benefit Questionnaire (NPPBQ).Methods After expert consultation and nurse interviews, a primary questionnaire was developed for an exploratory factor analysis (EFA). The seventeen items of the NPPBQ were used for verification of the theorized factor structure and content validity using a confirmatory factor analysis (CFA). The NPPBQ’s concurrent validity was evaluated. Three samples of nurses were collected in Shanghai, Hangzhou and Nanjing between November 2017 and August 2018.Results The results of the EFA and CFA verified the five dimensions of nurses’ occupational benefit discovery. The results demonstrated that the NPPBQ has adequate internal consistency and is fully consistent with the theorized factor structure. This 5-factor solution explained an adequate percentage of the total variance. The Cronbach’s alpha of each dimension of the NPPBQ was good. The concurrent validity was significantly correlated with all aspects of the Maslach Burnout Inventory (MBI).Conclusion The results suggest that the NPPBQ is a psychometrically sound measure for evaluating perceived professional benefits among a wide range of nurses.
Efficacy of acupuncture in improving the symptoms and life quality of patients with moderate or severe acne vulgaris: protocol for a randomized controlled trial
Authors: Jiao Ruimin , Huang Man , Zhang Weina , Liu Zhishun 
Published: January 2020

Abstract
Background: Acne vulgaris (AV) is a common chronic dermatologic disease that tends to impair the appearance and quality of life (QoL) of patients. Although acupuncture has been indicated as an effective treatment for patients with AV in several trials, the results of these trials are also controversial due to potential for bias of their design. And none of the previous clinical trials of acupuncture therapy for AV used the QoL as a primary outcome or employed a sham acupuncture control arm. The aim of the study is to evaluate the effect of acupuncture on the symptoms and QoL of patients with moderate or severe AV. Methods/design: Ninety-six eligible participants with AV will be randomly assigned to acupuncture or sham acupuncture groups (1:1 allocation). All participants will receive 4-week treatment comprising of a total of 12 sessions (3 sessions per week). The primary outcome will be change from baseline in the Skindex-16 scale total score at treatment completion. The secondary outcomes will be Skindex-16 subscale scores, the Dermatology Life Quality Index scale total score, the total lesion count and inflammatory lesion count, itch and pain assessment using the visual analogue scale score, patient expectations of acupuncture, and the blinding of the effect of sham acupuncture. Follow-up evaluation will be performed at weeks 16 and 28. All outcome analyses will be performed in the intention-to-treat population. Discussion: We expect to evaluate the effectiveness of acupuncture on the symptoms and QoL of patients with moderate or severe AV compared with sham acupuncture. The limitation of the design of this trial is that the single center study with a relatively small sample size, the acupuncturists will not be blinded.
Detection of aberrant events in RNA sequencing data
Authors: Yépez Vicente A. , Mertes Christian , Mueller Michaela F. , Andrade Daniela S. , Wachutka Leonhard , Frésard Laure , Gusic Mirjana , Scheller Ines , Goldberg Patricia F. , Prokisch Holger , Gagneur Julien 
Published: January 2020

Abstract
RNA sequencing (RNA-seq) has emerged as a powerful approach to discover disease-causing gene regulatory defects for individuals affected with a genetically undiagnosed rare disorder. Pioneer studies have shown that RNA-seq could increase diagnostic rates over DNA sequencing alone by 8% to 36 % depending on disease entities and probed tissues. To accelerate the adoption of RNA-seq among human genetic centers, detailed analysis protocols are now needed. Here, we present a step-by-step protocol that instructs how to robustly detect aberrant expression, aberrant splicing, and mono-allelic expression of a rare allele in RNA-seq data using dedicated statistical methods. We describe how to generate and assess quality control plots and interpret the analysis results. The protocol is based on DROP (Detection of RNA Outliers Pipeline), a modular computational workflow that integrates all analysis steps, can leverage parallel computing infrastructures, and generates browsable web page reports.
General trends in freshwater ecological restoration practice in China over the past two decades: the driving factors and the evaluation of restoration outcome
Authors: Wu Juan , Dai Yanran , Cheng Shuiping 
Published: January 2020

Abstract
Background Although freshwater ecological restoration (FER) has undergone an immense development in China either in the number of projects or in the spatial scale of implementations, a dearth of clear and comprehensive trends in this field is still a particular concern. We conducted a literature survey through searching the database of Web of Science between 1998-2017.Results A total of 2047 publications were hit and 198 of them were finally retained after manual screening. The number of studies in this field has been steadily increasing in recent years and their provincial distribution is positively correlated with GDP growth and the investment to pollution control and protection, suggesting that economic development is a key driving factor of FER practice. Among the remained articles, nearly half (46.5%) focus on lake ecosystems, and 34.8% and 32.8% of the studies believe that land reclamation and eutrophication are the top two causes of freshwater ecosystem degradation. The overarching target of the restoration is biodiversity increase (31.4%), followed by water quality improvement (24.7%) and ecosystem services (23.9%). Revegetation is the dominant restoration approach (40.9%). Reference sites for assessment of restoration projects are normally control areas or locations without intervention (60%), or the status of the targeted sites before the interventions. For the restoration outcome evaluation, 86% of the studies present positive outcomes in terms of water quality improvement, and 79% have improvement in biological features. The most frequently monitored organisms are macrophytes (31%), then followed by benthos as indicators of ecological condition.Conclusions The literature research indicated that economic growth, water pollution and investment into environmental protection are the main driving factors of FER practice in China. Additionally, the effort of restoration and evaluation over the past two decades has not been limited to improving hydrological function and water quality, but also pay increasingly more attention to biological processes and ecological integrity, and further the ecosystem services in recent years. However, the lack of long-term monitoring and socioeconomic attributes considered in restoration success assessments are still particular issues needed to be addressed in the future FER researches and projects.
Yeast display platform technology to prepare oral vaccine against lethal H7N9 virus challenge in mice
Authors: Lei Han , Xie Bowen , Gao Tong , Cen Qianhong , Ren Yi 
Published: January 2020

Abstract
Background Existing methods for preparing influenza vaccines pose the greatest challenge against the threat of highly pandemic avian influenza virus H7N9 outbreak in the poultry and human. Exploring a new strategy for manufacturing and delivering a safe and effective H7N9 vaccine is urgently needed. Results An alternative approach, proposed here, is to develop influenza an oral H7N9 vaccine based on yeast display technology in a timely manner. Hemagglutinin (HA) of A/Anhui/1/2013 (AH-H7N9) as a model antigen and characterized its expression on the surface of Saccharomyces cerevisiae ( S.cerevisiae ) EBY 100. Mice orally administrated with S.cerevisiae EBY100/pYD5-HA produced significant titers of IgG antibody as well as significant amounts of cytokines IFN-γ and IL-4. Importantly, S.cerevisiae EBY100/pYD5-HA could provide complete immune protection against homologous A/Anhui/1/2013 (AH-H7N9) virus challenge. Conclusion Our findings suggest that platform based on yeast surface technology provides an alternative approach to prepare a promising influenza H7N9 vaccine candidate that can significantly shorten the preparedness period and result in effective protection against influenza A pandemic. Keywords: S.cerevisiae EBY100/pYD5-HA, Yeast display technology, Influenza A pandemic.