bluuebunny/crossref_metadata_2025_split huggingface dataset:
Posterior basolateral amygdala to ventral hippocampal CA1 drives approach behaviour to exert an anxiolytic effect
Authors: Pi Guilin , Gao Di , Wu Dongqin , Wang Yali , Lei Huiyang , Zeng Wenbo , Gao Yang , Yu Huiling , Xiong Rui , Jiang Tao , Li Shihong , Wang Xin , Guo Jing , Zhang Si , Yin Taoyuan , He Ting , Ke Dan , Li Ruining , Li Honglian , Liu Gongping , Yang Xifei , Luo Min–Hua , Zhang Xiaohui , Yang Ying , Wang Jian–Zhi 
Published: January 2020

AbstractThe basolateral amygdala (BLA) and ventral hippocampal CA1 (vCA1) are cellularly and functionally diverse along their anterior–posterior and superficial-deep axes. Here, we find that anterior BLA (aBLA) and posterior BLA (pBLA) innervate deep-layer calbindin1-negative (Calb1−) and superficial-layer calbindin1-positive neurons (Calb1+) in vCA1, respectively. Photostimulation of pBLA–vCA1 inputs has an anxiolytic effect in mice, promoting approach behaviours during conflict exploratory tasks. By contrast, stimulating aBLA–vCA1 inputs induces anxiety-like behaviour resulting in fewer approaches. During conflict stages of the elevated plus maze task vCA1Calb1+ neurons are preferentially activated at the open-to-closed arm transition, and photostimulation of vCA1Calb1+ neurons at decision-making zones promotes approach with fewer retreats. In the APP/PS1 mouse model of Alzheimer’s disease, which shows anxiety-like behaviour, photostimulating the pBLA–vCA1Calb1+ circuit ameliorates the anxiety in a Calb1-dependent manner. These findings suggest the pBLA–vCA1Calb1+ circuit from heterogeneous BLA–vCA1 connections drives approach behaviour to reduce anxiety-like behaviour.
Inhibition within a premotor circuit controls the timing of vocal turn-taking in zebra finches
Authors: Benichov Jonathan I. , Vallentin Daniela 
Published: January 2020

AbstractVocal turn-taking is a fundamental organizing principle of human conversation but the neural circuit mechanisms that structure coordinated vocal interactions are unknown. The ability to exchange vocalizations in an alternating fashion is also exhibited by other species, including zebra finches. With a combination of behavioral testing, electrophysiological recordings, and pharmacological manipulations we demonstrate that activity within a cortical premotor nucleus orchestrates the timing of calls in socially interacting zebra finches. Within this circuit, local inhibition precedes premotor neuron activation associated with calling. Blocking inhibition results in faster vocal responses as well as an impaired ability to flexibly avoid overlapping with a partner. These results support a working model in which premotor inhibition regulates context-dependent timing of vocalizations and enables the precise interleaving of vocal signals during turn-taking.
Comparative therapeutic efficacy of remdesivir and combination lopinavir, ritonavir, and interferon beta against MERS-CoV
Authors: Sheahan Timothy P. , Sims Amy C. , Leist Sarah R. , Schäfer Alexandra , Won John , Brown Ariane J. , Montgomery Stephanie A. , Hogg Alison , Babusis Darius , Clarke Michael O. , Spahn Jamie E. , Bauer Laura , Sellers Scott , Porter Danielle , Feng Joy Y. , Cihlar Tomas , Jordan Robert , Denison Mark R. , Baric Ralph S. 
Published: January 2020

AbstractMiddle East respiratory syndrome coronavirus (MERS-CoV) is the causative agent of a severe respiratory disease associated with more than 2468 human infections and over 851 deaths in 27 countries since 2012. There are no approved treatments for MERS-CoV infection although a combination of lopinavir, ritonavir and interferon beta (LPV/RTV-IFNb) is currently being evaluated in humans in the Kingdom of Saudi Arabia. Here, we show that remdesivir (RDV) and IFNb have superior antiviral activity to LPV and RTV in vitro. In mice, both prophylactic and therapeutic RDV improve pulmonary function and reduce lung viral loads and severe lung pathology. In contrast, prophylactic LPV/RTV-IFNb slightly reduces viral loads without impacting other disease parameters. Therapeutic LPV/RTV-IFNb improves pulmonary function but does not reduce virus replication or severe lung pathology. Thus, we provide in vivo evidence of the potential for RDV to treat MERS-CoV infections.
Melting of recycled ancient crust responsible for the Gutenberg discontinuity
Authors: Liu Jia , Hirano Naoto , Machida Shiki , Xia Qunke , Tao Chunhui , Liao Shili , Liang Jin , Li Wei , Yang Weifang , Zhang Guoying , Ding Teng 
Published: January 2020

AbstractA discontinuity in the seismic velocity associated with the lithosphere-asthenosphere interface, known as the Gutenberg discontinuity, is enigmatic in its origin. While partial mantle melts are frequently suggested to explain this discontinuity, it is not well known which factors critically regulate the melt production. Here, we report geochemical evidence showing that the melt fractions in the lithosphere-asthenosphere boundary were enhanced not only by accumulation of compacted carbonated melts related to recycled ancient marine sediments, but also by partial melting of a pyroxene-rich mantle domain related to the recycled oceanic eclogite/pyroxenites. This conclusion is derived from the first set of Mg isotope data for a suite of young petit-spot basalts erupted on the northwest Pacific plate, where a clearly defined Gutenberg discontinuity exists. Our results reveal a specific linkage between the Gutenberg discontinuity beneath the normal oceanic regions and the recycling of ancient subducted crust and carbonate through the deep Earth.
Strong inverse kinetic isotope effect observed in ammonia charge exchange reactions
Authors: Petralia L. S. , Tsikritea A. , Loreau J. , Softley T. P. , Heazlewood B. R. 
Published: January 2020

AbstractIsotopic substitution has long been used to understand the detailed mechanisms of chemical reactions; normally the substitution of hydrogen by deuterium leads to a slower reaction. Here, we report our findings on the charge transfer collisions of cold $${{\rm{Xe}}}^{+}$$

Xe

+

ions and two isotopologues of ammonia, $${{\rm{NH}}}_{3}$$

NH

3

and $${{\rm{ND}}}_{3}$$

ND

3

. Deuterated ammonia is found to react more than three times faster than hydrogenated ammonia. Classical capture models are unable to account for this pronounced inverse kinetic isotope effect. Moreover, detailed ab initio calculations cannot identify any (energetically accessible) crossing points between the reactant and product potential energy surfaces, indicating that electron transfer is likely to be slow. The higher reactivity of $${{\rm{ND}}}_{3}$$

ND

3

is attributed to the greater density of states (and therefore lifetime) of the deuterated reaction complex compared to the hydrogenated system. Our observations could provide valuable insight into possible mechanisms contributing to deuterium fractionation in the interstellar medium.
Cold-induced urticarial autoinflammatory syndrome related to factor XII activation
Authors: Scheffel Jörg , Mahnke Niklas A. , Hofman Zonne L. M. , Maat Steven de , Wu Jim , Bonnekoh Hanna , Pengelly Reuben J. , Ennis Sarah , Holloway John W. , Kirchner Marieluise , Mertins Philipp , Church Martin K. , Maurer Marcus , Maas Coen , Krause Karoline 
Published: January 2020

AbstractHereditary autoinflammatory diseases are caused by gene mutations of the innate immune pathway, e.g. nucleotide receptor protein 3 (NLRP3). Here, we report a four-generation family with cold-induced urticarial rash, arthralgia, chills, headache and malaise associated with an autosomal-dominant inheritance. Genetic studies identify a substitution mutation in geneF12(T859A, resulting in p.W268R) which encodes coagulation factor XII (FXII). Functional analysis reveals enhanced autocatalytic cleavage of the mutated protein and spontaneous FXII activation in patient plasma and in supernatant of transfected HEK293 cells expressing recombinant W268R-mutated proteins. Furthermore, we observe reduced plasma prekallikrein, cleaved high molecular weight kininogen and elevated plasma bradykinin. Neutrophils are identified as a local source of FXII. Interleukin-1β (IL-1β) is upregulated in lesional skin and mononuclear donor cells exposed to recombinant mutant proteins. Treatment with icatibant (bradykinin-B2-antagonist) or anakinra (interleukin-1-antagonist) reduces disease activity in patients. In conclusion, our findings provide a link between contact system activation and cytokine-mediated inflammation.
Reconciling the disagreement between observed and simulated temperature responses to deforestation
Authors: Chen Liang , Dirmeyer Paul A. 
Published: January 2020

AbstractLand use changes have great potential to influence temperature extremes. However, contradictory summer daytime temperature responses to deforestation are reported between observations and climate models. Here we present a pertinent comparison between multiple satellite-based datasets and climate model deforestation experiments. Observationally-based methods rely on a space-for-time assumption, which compares neighboring locations with contrasting land covers as a proxy for land use changes over time without considering possible atmospheric feedbacks. Offline land simulations or subgrid-level analyses agree with observed warming effects only when the space-for-time assumption is replicated. However, deforestation-related cloud and radiation effects manifest in coupled climate simulations and observations at larger scales, which show that a reduction of hot extremes with deforestation – as simulated in a number of CMIP5 models – is possible. Our study provides a design and analysis methodology for land use change studies and highlights the importance of including land-atmosphere coupling, which can alter deforestation-induced temperature changes.
Colloidal interactions and unusual crystallization versus de-mixing of elastic multipoles formed by gold mesoflowers
Authors: Yuan Ye , Tasinkevych Mykola , Smalyukh Ivan I. 
Published: January 2020

AbstractColloidal interactions in nematic liquid crystals can be described as interactions between elastic multipoles that depend on particle shape, topology, chirality, boundary conditions and induced topological defects. Here, we describe a nematic colloidal system consisting of mesostructures of gold capable of inducing elastic multipoles of different order. Elastic monopoles are formed by relatively large asymmetric mesoflower particles, for which gravity and elastic torque balancing yields monopole-type interactions. High-order multipoles are instead formed by smaller mesoflowers with a myriad of shapes corresponding to multipoles of different orders, consistent with our computer simulations based on free energy minimization. We reveal unexpected many-body interactions in this colloidal system, ranging from de-mixing of elastic monopoles to a zoo of unusual colloidal crystals formed by high-order multipoles like hexadecapoles. Our findings show that gold mesoflowers may serve as a designer toolkit for engineering colloidal interaction and self-assembly, potentially exceeding that in atomic and molecular systems.
Mechanical breathing in organic electrochromics
Authors: Wang Xiaokang , Chen Ke , de Vasconcelos Luize Scalco , He Jiazhi , Shin Yung C. , Mei Jianguo , Zhao Kejie 
Published: January 2020

AbstractThe repetitive size change of the electrode over cycles, termed as mechanical breathing, is a crucial issue limiting the quality and lifetime of organic electrochromic devices. The mechanical deformation originates from the electron transport and ion intercalation in the redox active material. The dynamics of the state of charge induces drastic changes of the microstructure and properties of the host, and ultimately leads to structural disintegration at the interfaces. We quantify the breathing strain and the evolution of the mechanical properties of poly(3,4-propylenedioxythiophene) thin films in-situ using customized environmental nanoindentation. Upon oxidation, the film expands nearly 30% in volume, and the elastic modulus and hardness decrease by a factor of two. We perform theoretical modeling to understand thin film delamination from an indium tin oxide (ITO) current collector under cyclic load. We show that toughening the interface with roughened or silica-nanoparticle coated ITO surface significantly improves the cyclic performance.
Cervical epithelial damage promotes Ureaplasma parvum ascending infection, intrauterine inflammation and preterm birth induction in mice
Authors: Pavlidis Ioannis , Spiller Owen B. , Sammut Demarco Gabriella , MacPherson Heather , Howie Sarah E. M. , Norman Jane E. , Stock Sarah J. 
Published: January 2020

AbstractAround 40% of preterm births are attributed to ascending intrauterine infection, andUreaplasma parvum(UP) is commonly isolated in these cases. Here we present a mouse model of ascending UP infection that resembles human disease, using vaginal inoculation combined with mild cervical injury induced by a common spermicide (Nonoxynol-9, as a surrogate for any mechanism of cervical epithelial damage). We measure bacterial load in a non-invasive manner using a luciferase-expressing UP strain, and post-mortem by qPCR and bacterial titration. Cervical exposure to Nonoxynol-9, 24 h pre-inoculation, facilitates intrauterine UP infection, upregulates pro-inflammatory cytokines, and increases preterm birth rates from 13 to 28%. Our results highlight the crucial role of the cervical epithelium as a barrier against ascending infection. In addition, we expect the mouse model will facilitate further research on the potential links between UP infection and preterm birth.
Plant metabolism of nematode pheromones mediates plant-nematode interactions
Authors: Manohar Murli , Tenjo-Castano Francisco , Chen Shiyan , Zhang Ying K. , Kumari Anshu , Williamson Valerie M. , Wang Xiaohong , Klessig Daniel F. , Schroeder Frank C. 
Published: January 2020

AbstractMicroorganisms and nematodes in the rhizosphere profoundly impact plant health, and small-molecule signaling is presumed to play a central role in plant rhizosphere interactions. However, the nature of the signals and underlying mechanisms are poorly understood. Here we show that the ascaroside ascr#18, a pheromone secreted by plant-parasitic nematodes, is metabolized by plants to generate chemical signals that repel nematodes and reduce infection. Comparative metabolomics of plant tissues and excretions revealed that ascr#18 is converted into shorter side-chained ascarosides that confer repellency. An Arabidopsis mutant defective in two peroxisomal acyl-CoA oxidases does not metabolize ascr#18 and does not repel nematodes, indicating that plants, like nematodes, employ conserved peroxisomal β-oxidation to edit ascarosides and change their message. Our results suggest that plant-editing of nematode pheromones serves as a defense mechanism that acts in parallel to conventional pattern-triggered immunity, demonstrating that plants may actively manipulate chemical signaling of soil organisms.
Differential modulation of NREM sleep regulation and EEG topography by chronic sleep restriction in mice
Authors: Kim Bowon , Hwang Eunjin , Strecker Robert E. , Choi Jee Hyun , Kim Youngsoo 
Published: January 2020

AbstractCompensatory elevation in NREM sleep EEG delta power has been typically observed following prolonged wakefulness and widely used as a sleep homeostasis indicator. However, recent evidence in human and rodent chronic sleep restriction (CSR) studies suggests that NREM delta power is not progressively increased despite of accumulated sleep loss over days. In addition, there has been little progress in understanding how sleep EEG in different brain regions responds to CSR. Using novel high-density EEG electrode arrays in the mouse model of CSR where mice underwent 18-h sleep deprivation per day for 5 consecutive days, we performed an extensive analysis of topographical NREM sleep EEG responses to the CSR condition, including period-amplitude analysis of individual slow waves. As previously reported in our analysis of REM sleep responses, we found different patterns of changes: (i) progressive decrease in NREM sleep duration and consolidation, (ii) persistent enhancement in NREM delta power especially in the frontal and parietal regions, and (iii) progressive increases in individual slow wave slope and frontal fast oscillation power. These results suggest that multiple sleep-wake regulatory systems exist in a brain region-specific manner, which can be modulated independently, especially in the CSR condition.
Calcite incorporated in silica/collagen xerogels mediates calcium release and enhances osteoblast proliferation and differentiation
Authors: Rößler S. , Unbehau R. , Gemming T. , Kruppke B. , Wiesmann H.-P. , Hanke T. 
Published: January 2020

AbstractMultiphasic silica/collagen xerogels are biomaterials designed for bone regeneration. Biphasic silica/collagen xerogels (B30) and triphasic xerogels (B30H20 or B30CK20) additionally containing hydroxyapatite or calcite were demonstrated to exhibit several structural levels. On the first level, low fibrillar collagen serves as template for silica nanoparticle agglomerates. On second level, this silica-enriched matrix phase is fiber-reinforced by collagen fibrils. In case of hydroxyapatite incorporation in B30H20, resulting xerogels exhibit a hydroxyapatite-enriched phase consisting of hydroxyapatite particle agglomerates next to silica and low fibrillar collagen. Calcite in B30CK20 is incorporated as single non-agglomerated crystal into the silica/collagen matrix phase with embedded collagen fibrils. Both the structure of multiphasic xerogels and the manner of hydroxyapatite or calcite incorporation have an influence on the release of calcium from the xerogels. B30CK20 released a significantly higher amount of calcium into a calcium-free solution over a three-week period than B30H20. In calcium containing incubation media, all xerogels caused a decrease in calcium concentration as a result of their bioactivity, which was superimposed by the calcium release for B30CK20 and B30H20. Proliferation of human bone marrow stromal cells in direct contact to the materials was enhanced on B30CK20 compared to cells on both plain B30 and B30H20.
Multiple gene promoter methylation and clinical stage in adjacent normal tissues: Effect on prognosis of colorectal cancer in Taiwan
Authors: Hsu Chih-Hsiung , Hsiao Cheng-Wen , Sun Chien-An , Wu Wen-Chih , Yang Tsan , Hu Je-Ming , Liao Yu-Chan , Huang Chi-Hua , Chen Chao-Yang , Lin Fu-Huang , Chou Yu-Ching 
Published: January 2020

AbstractThis study provide an insight that the panel genes methylation status in different clinical stage tended to reflect a different prognosis even in matched normal tissues, to clinical recommendation. We enrolled 153 colorectal cancer patients from a medical center in Taiwan and used the candidate gene approach to select five genes involved in carcinogenesis pathways. We analyzed the relationship between DNA methylation with different cancer stages and the prognostic outcome. There were significant trends of increasing risk of 5-year time to progression and event-free survival of subjects with raising number of hypermethylation genes both in normal tissue and tumor tissue. The group with two or more genes with aberrant methylation in the advanced cancer stages (Me/advanced) had lower 5-year event-free survival among patients with colorectal cancer in either normal or tumor tissue. The adjusted hazard ratios in the group with two or more genes with aberrant methylation with advanced cancer stages (Me/advanced) were 8.04 (95% CI, 2.80–23.1; P for trend <0.01) and 8.01 (95% CI, 1.92–33.4; P for trend <0.01) in normal and tumor tissue, respectively. DNA methylation status was significantly associated with poor prognosis outcome. This finding in the matched normal tissues of colorectal cancer patients could be an alternative source of prognostic markers to assist clinical decision making.
Genetic Variants Associated with Chronic Kidney Disease in a Spanish Population
Authors: Corredor Zuray , Filho Miguel Inácio da Silva , Rodríguez-Ribera Lara , Velázquez Antonia , Hernández Alba , Catalano Calogerina , Hemminki Kari , Coll Elisabeth , Silva Irene , Diaz Juan Manuel , Ballarin José , Vallés Prats Martí , Calabia Martínez Jordi , Försti Asta , Marcos Ricard , Pastor Susana 
Published: January 2020

AbstractChronic kidney disease (CKD) patients have many affected physiological pathways. Variations in the genes regulating these pathways might affect the incidence and predisposition to this disease. A total of 722 Spanish adults, including 548 patients and 174 controls, were genotyped to better understand the effects of genetic risk loci on the susceptibility to CKD. We analyzed 38 single nucleotide polymorphisms (SNPs) in candidate genes associated with the inflammatory response (interleukins IL-1A, IL-4, IL-6, IL-10, TNF-α, ICAM-1), fibrogenesis (TGFB1), homocysteine synthesis (MTHFR), DNA repair (OGG1, MUTYH, XRCC1, ERCC2, ERCC4), renin-angiotensin-aldosterone system (CYP11B2, AGT), phase-II metabolism (GSTP1, GSTO1, GSTO2), antioxidant capacity (SOD1, SOD2, CAT, GPX1, GPX3, GPX4), and some other genes previously reported to be associated with CKD (GLO1, SLC7A9, SHROOM3, UMOD, VEGFA, MGP, KL). The results showed associations of GPX1, GSTO1, GSTO2, UMOD, and MGP with CKD. Additionally, associations with CKD related pathologies, such as hypertension (GPX4, CYP11B2, ERCC4), cardiovascular disease, diabetes and cancer predisposition (ERCC2) were also observed. Different genes showed association with biochemical parameters characteristic for CKD, such as creatinine (GPX1, GSTO1, GSTO2, KL, MGP), glomerular filtration rate (GPX1, GSTO1, KL, ICAM-1, MGP), hemoglobin (ERCC2, SHROOM3), resistance index erythropoietin (SOD2, VEGFA, MTHFR, KL), albumin (SOD1, GSTO2, ERCC2, SOD2), phosphorus (IL-4, ERCC4 SOD1, GPX4, GPX1), parathyroid hormone (IL-1A, IL-6, SHROOM3, UMOD, ICAM-1), C-reactive protein (SOD2, TGFB1,GSTP1, XRCC1), and ferritin (SOD2, GSTP1, SLC7A9, GPX4). To our knowledge, this is the second comprehensive study carried out in Spanish patients linking genetic polymorphisms and CKD.
Broadband Frequency Scanning Spoof Surface Plasmon Polariton Design with Highly Confined Endfire Radiations
Authors: Kandwal Abhishek , Li Jingzhen , Igbe Tobore , Liu Yuhang , Li Sinan , Wang Lei , Hao Yang , Nie Zedong 
Published: January 2020

AbstractThis paper proposes a compact broadband frequency scanning spoof surface plasmon polariton (SSPP) based design for efficient endfire radiations with high field confinement. Through the dispersion engineering, highly confined field distribution has been obtained in the operating frequency region. The proposed SSPP antenna has achieved a continuous through endfire scanning in the X-band at other operating frequencies which is in general difficult to obtain for SSPP based antennas. In the proposed design methodology, the swore-shaped surface plasmon antenna has both edges corrugated with an array of rectangular grooves which effectively confines the electromagnetic field into a slow travelling wave. The surface impedances along both edges were engineered to be different at operating frequencies as to force the surface current to preferentially flow along either edge of the antenna to a different extent. The design with overall dimensions of (55 × 30) mm2 has achieved a broadband of 4 GHz with high peak measured gain of 9.8 dBi and peak efficiency of about 95 percent in the X-band. The antenna has been further tested experimentally for scanning application of target location also.