bluuebunny/crossref_metadata_2025_split huggingface dataset:
Human Decidua Basalis mesenchymal stem/stromal cells reverse the damaging effects of high level of glucose on endothelial cells in vitro
Authors: Basmaeil Yasser S. , Bahattab Eman. , Alshabibi Manal A. , Abomaray Fawaz M. , Abumaree Mohamed , Khatlani Tanvir 
Published: June 2020

AbstractRecently, we reported the therapeutic potential of mesenchymal stem/stromal cells (MSCs) from the maternal decidua basalis tissue of human term placenta (DBMSCs) to treat inflammatory diseases, such as atherosclerosis and cancer. DMSCs protect endothelial cell functions from the negative effects of oxidative stress mediators including hydrogen peroxide (H2O2) and monocytes. In addition, DBMSCs induce the generation of anti‐cancer immune cells known as M1 macrophages. Diabetes is another inflammatory disease where endothelial cells are injured by H2O2 produced by high level of glucose (hyperglycaemia), which is associated with development of thrombosis. Here, we investigated the ability of DBMSCs to reverse the damaging effects of high levels of glucose on endothelial cells. DBMSCs and endothelial cells were isolated from human placental and umbilical cord tissues, respectively. Endothelial cells were incubated with glucose in presence of DBMSCs, and their functions were evaluated. The effect of DBMSCs on glucose‐ treated endothelial cell expression of genes was also determined. DBMSCs reversed the effects of glucose on endothelial cell functions including proliferation, migration, angiogenesis and permeability. In addition, DBMSCs modified the expression of several genes mediating essential endothelial cell functions including survival, apoptosis, permeability and angiogenesis. We report the first evidence that DBMSCs protect the functions of endothelial cells from the damaging effects of glucose. Based on these results, we establish that DBMSCs are promising therapeutic agents to repair glucose‐induced endothelial cell injury in diabetes. However, these finding must be investigated further to determine the pathways underlying the protective role of DBMSCs on glucose‐stimulated endothelial cell Injury.
Effects of three abnormal conditions of fallopian tube on outcomes of the in vitro fertilization and embryo transfer technique
Authors: Liu Chengjun , Su Ketong , Tian Meng , Ji Huili , Sun Lina , Li Chunjin , Zhou Xu 
Published: June 2020

AbstractAimThe aim of this study was to demonstrate whether salpingitis affects the outcomes of in vitro fertilization and embryo transfer (IVF‐ET).MethodsThe retrospective study includes patients from 2013 to 2018 who received their first IVF‐ET treatment during this period. On the basis of their tubal conditions, the patients were subgrouped as: hydrosalpinx (group A), salpingitis (group B), tubal occlusion (group C). It had a total of 726 cycles, of which 208 cycles were in group A, 201 cycles in group B and 317 cycles in group C. The outcomes of the IVF‐ET treatment were compared amongst the three groups.ResultsGroup C had the highest number of retrieved oocytes as compared to the groups A and B, and the rate of the high‐quality embryos at day 3 (66–68 h after insemination) was higher in the groups C and A compared to the group B. The blastocyst formation rate was significantly higher in group C compared to that of the group B. Group C had higher rates of implantation, clinical pregnancy and live birth compared to both groups A and B, while the birth weight of newborns did not differ amongst the three groups.ConclusionSalpingitis has adverse effects on the success rate of the IVF‐ET treatment, exemplified by lower implantation, clinical pregnancy and live birth rates compared to tubal occlusion, it may be necessary to carry out appropriate management of salpingitis before IVF‐ET treatment.
How safe is high‐order repeat cesarean delivery? An 8‐year single‐center experience in Lebanon
Authors: Ramadan Mohamad K. , Ramadan Khalil , El Tal Rana , Salem Wehbe Georges R. , Itani Saadeddine , Badr Dominique A. 
Published: June 2020

AbstractAimTo quantify the impact of the number of prior cesarean deliveries (CD) on operative complications and preterm birth. Then to investigate the presence of a threshold, beyond which complications tend to be disproportionately dangerous.MethodsThis was a retrospective cohort observational study, where data corresponding to all CD done at our service, during an 8‐year period, were collected and analyzed. In total, 1840 CD were performed. Patients were divided into five categories that corresponded to the number of CD. Primary outcome was the composite adverse maternal outcome, while preterm birth and individual complications were secondary outcomes.ResultsThe composite adverse maternal outcome, preterm birth, as well as all individual complications related to CD, except for placental abruption, showed a significant rise in frequency that paralleled the increase in the number of CD. Furthermore, this increase tended to be continuous as the number of CD increased, with an evident surge after the fourth.ConclusionIn our population, increasing number of prior CD was a risk factor for a parallel increase in the rate of composite adverse maternal outcome, preterm birth and almost all intraoperative complications attributable to CD. Decreasing exposure to such surgeries by limiting family size to four offspring should be considered seriously in patient counseling.
Differences in Travel Time to Cancer Surgery for Colon versus Rectal Cancer in a Rural State: A New Method for Analyzing Time‐to‐Place Data Using Survival Analysis
Authors: Matthews Kevin A. , Kahl Amanda R. , Gaglioti Anne H. , Charlton Mary E. 
Published: June 2020

AbstractPurposeRectal cancer is rarer than colon cancer and is a technically more difficult tumor for surgeons to remove, thus rectal cancer patients may travel longer for specialized treatment compared to colon cancer patients. The purpose of this study was to evaluate whether travel time for surgery was different for colon versus rectal cancer patients.MethodsA secondary data analysis of colorectal cancer (CRC) incidence data from the Iowa Cancer Registry data was conducted. Travel times along a street network from all residential ZIP Codes to all cancer surgery facilities were calculated using a geographic information system. A new method for analyzing “time‐to‐place” data using the same type of survival analysis method commonly used to analyze “time‐to‐event” data is introduced. Cox proportional hazard model was used to analyze travel time differences for colon versus rectal cancer patients.ResultsA total of 5,844 CRC patients met inclusion criteria. Median travel time to the nearest surgical facility was 9 minutes, median travel time to the actual cancer surgery facilities was 22 minutes, and the median number of facilities bypassed was 3. Although travel times to the nearest surgery facilities were not significantly different for colon versus rectal cancer patients, rectal cancer patients on average traveled 15 minutes longer to their actual surgery facility and bypassed 2 more facilities to obtain surgery.DiscussionIn general, the survival analysis method used to analyze the time‐to‐place data as described here could be applied to a wide variety of health services and used to compare travel patterns among different groups.
Transcriptomic plasticity of mesophotic corals among natural populations and transplants ofMontastraea cavernosain the Gulf of Mexico and Belize
Authors: Studivan Michael S. , Voss Joshua D. 
Published: June 2020

AbstractWhile physiological responses to low‐light environments have been studied among corals on mesophotic coral ecosystems worldwide (MCEs; 30–150 m), the mechanisms behind acclimatization and adaptation to depth are not well understood for most coral species. Transcriptomic approaches based on RNA sequencing are useful tools for quantifying gene expression plasticity, particularly in slow‐growing species such as scleractinian corals, and for identifying potential functional differences among conspecifics. A tag‐based RNA‐Seq (Tag‐Seq) pipeline was applied to quantify transcriptional variation in natural populations of the scleractinian coralMontastraea cavernosafrom mesophotic and shallower environments across five sites in Belize and the Gulf of Mexico: Carrie Bow Cay, West and East Flower Garden Banks, Pulley Ridge, and Dry Tortugas. Regional site location was a stronger driver of gene expression patterns than depth. However, mesophotic corals among all sites shared similar regulation of metabolic and cell growth functional pathways that may represent common physiological responses to environmental conditions at depth. Additionally, in a transplant experiment at West and East Flower Garden Banks, colonies transplanted from mesophotic to shallower habitats diverged from the control mesophotic group over time, indicating depth‐regulated plasticity of gene expression. When the shallower depth zone experienced a bleaching event, bleaching severity did not differ significantly between transplants and shallow controls, but gene expression patterns indicated variable regulation of stress responses among depth treatments. Coupled observational and experimental studies of gene expression among mesophotic and shallowerM. cavernosaprovide insights into the ability of this depth‐generalist coral species to persist under varying environmental conditions.
Barley strigolactone signalling mutant hvd14.d reveals the role of strigolactones in abscisic acid‐dependent response to drought
Authors: Marzec Marek , Daszkowska‐Golec Agata , Collin Anna , Melzer Michael , Eggert Kai , Szarejko Iwona 
Published: June 2020

AbstractStrigolactones (SLs) are a group of plant hormones involved in many aspects of plant development and stress adaptation. Here, we investigated the drought response of a barley (Hordeum vulgare L.) mutant carrying a missense mutation in the gene encoding the SL‐specific receptor HvD14. Our results clearly showed that hvd14.d mutant is hyper‐sensitive to drought stress. This was illustrated by a lower leaf relative water content (RWC), impaired photosynthesis, disorganization of chloroplast structure, altered stomatal density and slower closure of stomata in response to drought in the mutant compared to the wild type parent cultivar Sebastian. Although the content of abscisic acid (ABA) and its derivatives remained unchanged in the mutant, significant differences in expression of genes related to ABA biosynthesis were observed. Moreover, hvd14.d was insensitive to ABA during seed germination. Analysis of Arabidopsis thaliana mutant atd14‐1 also demonstrated that mutation in the SL receptor resulted in increased sensitivity to drought. Our results indicate that the drought‐sensitive phenotype of barley SL mutant might be caused by a disturbed ABA metabolism and/or signalling pathways. These results together uncovered a link between SL signalling and ABA‐dependent drought stress response in barley.
Facies and sequence stratigraphic architecture of the Mural Limestone (Albian), Arizona: Carbonate response to global and local factors and implications for reservoir characterization
Authors: Aisner‐Williams Rachel , Kerans Charles 
Published: June 2020

AbstractThis study documents the detailed facies and sequence stratigraphic architecture of a multi‐cyclic patch‐reef and its associated ramp interior facies that formed during Oceanic Anoxic Event 1b in the Mural Limestone, Arizona, USA. Ramp interior facies are comprised of bedded wackestone/packstone, rudist build‐up and coral–algal patch‐reef facies located north of Bisbee, Arizona, at the Grassy Hill locality. The larger multi‐cyclic patch‐reef that developed coevallyca5 km to the south of Grassy Hill consists of a high‐angle windward margin with a narrowca70 m long reef frame containing vertically zonatedMicrosolena, Actinastrea, diverse branching coral and rudist assemblages, and an 870 m long low‐angle leeward margin comprised of reef debris rudstone and grainstone shoal facies. Similar reef geomorphology and orientation is documented across the Gulf of Mexico and reflects the shelf‐wide north to north‐east‐trending prevailing wind and current energies. Controls affecting reef formation and growth patterns include changes in accommodation space associated with low‐amplitude global sea‐level rise and regional thermotectonic subsidence, local accommodation space and nutrient fluctuations associated with the inner shelf depositional setting within a humid and siliciclastic‐rich environment. Four aggradational to retrogradational high‐frequency sequences are documented in Arizona: High‐frequency sequences 1 and 2 represent the first pulse of patch‐reef development in an overall second‐order marine transgression over the Sonora/Bisbee Shelf. These sequences correlate to δ13C signatures associated with Oceanic Anoxic Event 1b across the Gulf of Mexico and suggest that carbonate reefs persisted on the ramp interior during this time. High‐frequency sequences 3 and 4 record a second brief transgression and backstepping of reef facies followed by the final regression of shallow shelf carbonates that correlates to more robust patch‐reef development in Sonora, Mexico. The patch‐reef at Paul Spur is an excellent outcrop analogue for productive patch‐reefs in the Maverick Basin (Comanche Shelf) of Texas. Detailed facies mapping of this outcrop analogue shows that the greatest reservoir potential is contained within the backreef grainstone shoals where primary porosity of up to 15% is observed.
Mobile communication and refugees: An analytical review of academic literature
Authors: Alencar Amanda 
Published: June 2020

AbstractOver the past decade, an expanding literature has explored the ways in which refugees rely on mobile communication technologies to stay in touch with a wider community and to access relevant information and services in their new places. Nevertheless, challenges linked to (a lack of) digital literacy and accessibility among refugees, and associated risks of technology use can negatively affect their empowerment and participation in a “mediatized” society. In light of the mutually shaping processes emerging between technology and society, this article provides an overview of studies focusing on the relationship between mobile communication practices and refugee lives under different circumstances. Using the concept of affordances as an analytic tool, this article looks into ways in which existing studies address the possibilities and vulnerabilities of mobile communications, the social conditions, and the agency of refugees in engaging with mobile technologies in the different temporal and spatial dimensions of their migration trajectories. Future studies should explore more collective processes and the power dynamics involved in the appropriation of mobile technologies by different migration actors.
Coronavirus disease 2019 in an orthotopic liver transplant recipient living with human immunodeficiency virus
Authors: Modi Anita R. , Koval Christine E. , Taege Alan J. , Modaresi Esfeh Jamak , Eghtesad Bijan , Narayanan Menon K. V. , Quintini Cristiano , Miller Charles 
Published: June 2020

AbstractCoronavirus disease 2019 (COVID‐19), mediated by severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2), can manifest with flu‐like illness and severe pneumonia with acute respiratory distress syndrome (ARDS). Immunocompromised patients merit particular attention as altered host immunity may influence both disease severity and duration of viral shedding as is described with several other ribonucleic acid respiratory viruses. Yet immunocompromised status alone, in the absence of other comorbidities, may not necessarily predict severe illness presentations and poorer clinical outcomes as indicated by recent reports of COVID‐19‐infected solid organ transplant recipients and people living with human immunodeficiency virus (HIV). Such patients may even be spared the robust inflammatory response that precipitates ARDS associated with COVID‐19, complicating the management of iatrogenic immunosuppression in this setting. We present a case of an orthotopic liver transplant recipient with well‐controlled HIV who successfully recovered from a mild, flu‐like illness attributed to SARS‐CoV‐2.
Clinicopathological features of fast eGFR decliners among patients with diabetic nephropathy
Authors: Furuichi Kengo , Shimizu Miho , Yamanouchi Masayuki , Hoshino Junichi , Sakai Norihiko , Iwata Yasunori , Toyama Tadashi , Kitajima Shinji , Hara Akinori , Yuzawa Yukio , Kitamura Hiroshi , Suzuki Yoshiki , Sato Hiroshi , Uesugi Noriko , Ueda Yoshihiko , Nishi Shinichi , Nishino Tomoya , Samejima Kenichi , Kohagura Kentaro , Shibagaki Yugo , Makino Hirofumi , Matsuo Seiichi , Ubara Yoshifumi , Yokoyama Hitoshi , Wada Takashi 
Published: June 2020

IntroductionThe speed of declining kidney function differs among patients with diabetic nephropathy. This study was undertaken to clarify clinical and pathological features that affect the speed of declining kidney function in patients with diabetic nephropathy.Research design and methodsThis study was design as multicenter retrospective study. The subjects (377 patients with diabetic nephropathy diagnosed by kidney biopsy at 13 centers in Japan) were classified into three groups based on the estimated glomerular filtration rate (eGFR) declining speed. The eGFR increasing group, the control group, and the eGFR declining group were divided at 0 and 5 mL/min/1.73 m2/year, respectively. Characteristics of clinicopathological findings of declining kidney function were evaluated.ResultsThe mean observation period of this study was 6.9 years. The control group, the eGFR increasing group, and the eGFR declining group included 81, 66, and 230 patients, respectively. The incidences of composite kidney events represented by 100 persons/year were 25.8 in the eGFR declining group and 2.0 in the eGFR increasing group. After adjustment for age, sex, systolic blood pressure, hemoglobin, and urinary albumin levels, three clinicopathological findings (urinary albumin levels, presence of nodular lesion, and mesangiolysis) were risk factors for inclusion in the eGFR declining group (the ORs were 1.49, 2.18, and 2.08, respectively). In contrast, the presence of subendothelial space widening and polar vasculosis were characteristic findings for inclusion in the eGFR increasing group (the ORs were 0.53 and 0.41, respectively).ConclusionsAs well as urinary albumin elevation, nodular lesion and mesangiolysis were characteristic pathological features of patients with fast declining kidney function.
Income and housing satisfaction and their association with self-rated health in different life stages. A fixed effects analysis using a German panel study
Authors: Knöchelmann Anja , Seifert Nico , Günther Sebastian , Moor Irene , Richter Matthias 
Published: June 2020

ObjectiveWe aim to investigate the effect of income and housing satisfaction on self-rated health in different life stages.DesignA population-based panel study (German Socio-Economic Panel).ParticipantsThe final sample consisted of 384 280 observations from 50 004 persons covering the period between 1994 and 2016.Outcome measuresAverage marginal effects were calculated based on fixed effects regressions to obtain the effect of changes in income and housing satisfaction on changes in self-rated health for each year of age. Self-rated health was assessed on a 5-point scale, with higher values indicating better health.ResultsChanges in income and housing satisfaction showed a small association with changes in self-rated health. The association was stronger for income, where it also varied considerably in different life stages. The average marginal effects for income satisfaction varied between 0.02 and 0.05 in men and 0.02 and 0.04 in women and peaked between the ages of 55–60. For housing satisfaction, average marginal effects ranged from 0.02 to 0.04 (men) and from 0.02 to 0.03 (women).ConclusionHigher satisfaction with housing and income was associated with better self-rated health. Therefore, studies on the social determinants of health should not only focus on objective material conditions but also on how individuals perceive and evaluate their situation.
Clostridial diseases in farm animals: 2. Histotoxic and neurotoxic diseases
Authors: Otter Arthur , Uzal Francisco A. 
Published: June 2020

Background: Clostridia are widespread in the environment, and consequently there is the potential to cause diseases in all farm species, affecting animals at pasture and those that are housed. It is essential that clostridial diseases are considered by practitioners with farm animal clients and prevention strategies included in livestock health plans.Aim of the article: This article, the second in a two‐part series on clostridial diseases in farm animals, discusses the range of histotoxic and neurotoxic clostridial diseases that is recognised in farm animals. The first part, which was published in the May issue of In Practice, reviewed clostridial enterotoxaemias caused by Clostridium perfringens, and other alimentary tract infections.
Circulating CD14highCD16lowintermediate blood monocytes as a biomarker of ascites immune status and ovarian cancer progression
Authors: Prat Mélissa , Le Naour Augustin , Coulson Kimberley , Lemée Fanny , Leray Hélène , Jacquemin Godefroy , Rahabi Mouna Chirine , Lemaitre Léa , Authier Hélène , Ferron Gwenaël , Barret Jean-Marc , Martinez Alejandra , Ayyoub Maha , Delord Jean-Pierre , Gladieff Laurence , Tabah-Fisch Isabelle , Prost Jean-François , Couderc Bettina , Coste Agnès 
Published: June 2020

BackgroundBesides the interest of an early detection of ovarian cancer, there is an urgent need for new predictive and prognostic biomarkers of tumor development and cancer treatment. In healthy patients, circulating blood monocytes are typically subdivided into classical (85%), intermediate (5%) and non-classical (10%) populations. Although these circulating monocyte subsets have been suggested as biomarkers in several diseases, few studies have investigate their potential as a predictive signature for tumor immune status,tumor growth and treatment adaptation.MethodsIn this study, we used a homogeneous cohort of 28 chemotherapy-naïve patients with ovarian cancer to evaluate monocyte subsets as biomarkers of the ascites immunological status. We evaluated the correlations between circulating monocyte subsets and immune cells and tumor burden in peritoneal ascites. Moreover, to validate the use of circulating monocyte subsets tofollow tumor progression and treatment response, we characterized blood monocytes from ovarian cancer patients included in a phase 1 clinical trial at baseline and following murlentamab treatment.ResultsWe demonstrate here a robust expansion of the intermediate blood monocytes (IBMs) in ovarian cancer patients. We establish a significant positive correlation between IBM percentage and tumor–associate macrophages with a CCR2high/CD163high/CD206high/CD86lowprofile. Moreover, IBM expansion is associated with a decreased effector/regulatory T-cell ratio in ascites and with the presence of soluble immunosuppressive mediators. We also establish that IBM proportion positively correlates with the peritoneum tumor burden. Finally, the study of IBMs in patients with ovarian cancer under immunotherapy during the phase clinical trial supports IBMs to follow the evolution of tumor development and the treatment adaptation.ConclusionsThis study, which links IBM level with immunosuppression and tumor burden in peritoneum, identifies IBMs as apotential predictive signature of ascites immune status and as a biomarker ofovarian cancer development and treatment response.Trial registration numberEudraCT: 2015-004252-22NCT02978755.