bluuebunny/crossref_metadata_2025_split huggingface dataset:
Influence of mechanical correction on the geometric and mechanical properties of cold-formed periodic profile fittings
Authors: Loktionova L. V. 
Published: June 2020

The article describes the process of mechanical correction of cold-formed fittings produced in coils on industrial rightsized cutting machines of rotary and roller types. The main qualitative characteristics of the reinforcement are given, such as: ultimate tensile strength, offset yield stress, the ratio of ultimate tensile strength offset yield stress, conditional yield strength, total elongation at maximum load and the relative area of crumpling. To assess the effect of mechanical correction on the properties of cold-formed periodic profile fittings, statistical data is used for testing samples of fittings produced in coils and straightened before manual testing and samples of the same fittings transferred from consumer companies after mechanical correction on right-sized rotary type cut machines.Tensile testing of samples was performed on testing machines in the laboratory of physical and mechanical tests No. 3 of the OJSC «BSW – Management Company of the Holding «BMC» in accordance with the requirements of ISO 15630-1:2010 «Steel for concrete reinforcement and pre-stressing. Test method. Part 1. Reinforcing rods, wire rod and wire» and ISO 6892:2016 «Metal Materials. Tensile test. Part 1. Method of test at ambient room temperature».
Thermodynamic metal crystallization basics
Authors: Marukovich E. I. , Stetsenko V. Yu. 
Published: June 2020

On the basis of thermodynamic calculations it is shown that crystallization of metals is a thermodynamic process, which takes place mainly at constant temperature. The exception is crystallization at very high cooling rates of the metal melt when the released solidification heat is not enough to stabilize the crystallization temperature of the liquid metal. In crystallization, the specific interfacial surface energy of crystals is not a constant value, but is proportional to their dimensions (bend radius).Nanocrystals of crystallizing phases exist in the metal melt steadily. Metal crystallization aggregates nanocrystals and free metal melt atoms into microcrystals. Mechanism of dendritic crystallization of metals is proposed.
PREVALENCE AND RISK FACTOR OF STONGYLOIDOSIS IN BEEF CATTLE IN KASIMAN SUBDISTRICT, BOJONEGORO
Authors: Ridwan Yusuf , Satrija Fadjar , Winarso Aji 
Published: June 2020

A pair of cross-sectional studies was conducted to determine prevalence and risk factors of Strongyloides spp. (threadworm) infections in beef cattle in Kasiman Subdistrict, the Regency of Bojonegoro. A total of 263 and 270 of local beef cattle with various age were selected for faecal sample during dry season (August-October) and wet season (February-March), respectively. Feces were processed for counting the number of eggs per gram feces (EPG) using McMaster method. The prevalence of strongyloidosis was 4.56% and 7.04% in the dry season and the rainy season, respectively. The factors that associated with prevalence and intensity infection were age and sex of cattle. During the dry season and the rainy season, the calf and weaner group (1 year) was more susceptible for Strongyloides infection than cattle with older age and showed higher intensity of infection (based on EPG values). The bulls showed a higher risk and intensity of infection than cows.
SINGLE BULB GARLIC (Allium sativum) EXTRACT IMPROVE SPERM QUALITY IN HYPERLIPIDEMIA MALE MICE MODEL
Authors: Qadariah Nosi , Lestari Sri Rahayu , Rohman Fatchur 
Published: June 2020

The aim of this research was to find out the influence of single bulb garlic on sperm quality improvement in hyperlipidemia model of male mice. Male mice (Balb-C, 12 weeks, bw 21±5 g) were given high fat diet for 45 days until their body weight achieved 45±5 g. The mice were then divided into six groups. The mice in the first group (control group (N)) was fed with 7 g higrow pokphan 551 per day. The mice in the second group (control negative group) was fed with 7 g high fat diet (HFD), while the mice in the third group (control positive group) were treated with statin at dose of 0.91 mg. The mice in groups 4, 5, and 6 were treated with single bulb garlic extract at dose of 125 mg/kg bw, 250 mg/kg bw, and 500 mg/kg bw, respectively. On the 31st day, the male were dissected and cauda epididymis was taken and chopped in a sterile phosphat buffer saline (PBS) and observation conducted on sperm quality consisted of sperm count, sperm motility and sperm normality. The results indicated a significant increase in sperm count, motility and normality in mice treated with single bulb garlic extract at dose of 250 mg/kg bw.
PERANAN HEMAGLUTININ Escherichia coli DALAM PROSES ADHESI
Authors: Abrar Mahdi 
Published: June 2020

Adhesi merupakan tahap inisiasi dari proses kolonisasi bakteri. Kemampuan adhesi ini diperantarai oleh keberadaan hemaglutinin pada permukaan bakteri. Tujuan penelitian ini adalah melihat kemampuan hemaglutinin pada Escherichia coli dalam proses adhesi. Uji adhesi pada sel epitel pipi dilakukan dengan menggunakan metode Velentine-Weiggand. Hasil penelitian menunjukkan bahwa Escherichia coli yang memiliki hemaglutinin menunjukkan kemampuan adhesi yang jauh lebih baik yakni rata-rata 67,6 bakteri/sel dibandingkan dengan yang tidak memiliki hemaglutinin rata-rata 7,2 bakteri/sel. Kemampuan adhesi ini berkaitan dengan keberadaan hemaglutinin di permukaan bakteri.
PROFIL HORMON ESTROGEN DAN PROGESTERON PADA SIKLUS BERAHI KAMBING LOKAL (Estrogen and Progesterone Profile in Aceh Local Goat Estrous Cycle )
Authors: Siregar Tongku Nizwan 
Published: June 2020

The aim of this research was to find out the normal estrogen and progesterone profile in local goat estrous cycle. Five female Aceh local goats which the criteria clinically in good condition, have been giving birth before, 1.5-3.0 years old, and showed minimal twice regularly estrous cycle. All samples were synchronized with 0.5 ml cloprostenol (Estrone, Bioveta) using randomly infection pattern. The data obtained were analyzed descriptively. Blood serum was collected for examination of estrogen and progesterone content on 0, 7, 14, and 21 day of estrous cycle (day 0 of estrous cycle). Hormone analysis was conducted using ELISA. The results showed that estrogen and progesterone profile on day 0, 7, 14, and 21 were 62.21; 28.61; 29.84; 79.89 pg/ml and 0.0; 8.6; 14.7; 0.0 ng/ml respectively.Keywords: estrogen, progesterone, estrous cycle, Aceh local goats
Diagnóstico do Lixo Marinho e Ações de Educação Ambiental na Praia de Navegantes/SC
Authors: ROSA CRISTIANE , Widmer Walter 
Published: June 2020

O lixo marinho tem contribuído consideravelmente para a poluição marinha e é uma das tendências globais para a questão ambiental. Como resultado da poluição marinha tem-se os impactos à biota, impactos econômicos à pesca e os impactos visuais nas praias. É necessária a realização de estudos que identifiquem a composição, abundância e o comportamento desses detritos diante das variáveis meteorológicas afim de contribuir para que ações de prevenção e mitigação sejam realizadas de forma efetiva. O presente estudo teve como objetivo diagnosticar o lixo marinho na faixa de areia da Praia de Navegantes/SC quanto a sua composição e abundância, suas possíveis fontes, bem como a ocorrência de pellets plásticos, buscando ao final do estudo resultados que venham a contribuir para os órgãos ambientais locais definirem melhores ações de prevenção e mitigação do lixo marinho, como a elaboração de uma coleção didática de lixo marinho como ferramenta de educação ambiental. Foram identificados 509 itens, sendo a grande maioria dos itens representados por detritos plásticos (464 itens). Foram também observados um total de 50 pellets plásticos. A proximidade com os portos de Itajai e Navegantes permite considerar a atividade portuária como uma das possíveis fontes desses pellets. As prováveis origens dos resíduos foram em grande maioria “Usuário de praia”, seguido de “Origem indeterminada”. Para a elaboração da coleção didático-científica de lixo marinho optou-se pelo formato digital através das coleta de informações, dados, detritos marinhos e imagens feitas durante as amostragens.
Nonlinear Model Predictive Control of Steam-Assisted-Gravity-Drainage Well Operations for Real-Time Production Optimization
Authors: Patel Rajan G. , Trivedi Japan J. 
Published: June 2020

SummaryIn deep oil-sands deposits using the steam-assisted-gravity-drainage (SAGD) recovery process, real-time optimization (RTO) involves controlling optimum subcool to ensure steam conformance. Contemporary workflows use linear model predictive control (MPC) with oversimplified models that are inadequate to represent highly complex, spatially distributed, and nonlinear reservoir dynamics. In this research, two novel workflows using nonlinear MPC (NMPC) are proposed. The first workflow reduces an NMPC problem to linear MPC by estimating an equivalent linear model of a nonlinear black-box model in a mean-square-error sense. Another approach is to use nonlinear dynamic models explicitly for accurate prediction of the plant states and/or outputs. The resulting nonconvex, nonlinear cost optimization problem is solved using an interior-point algorithm at each control interval. Proposed workflows are tested using the history-matched, field-scale model of a SAGD reservoir located in northern Alberta, Canada. Qualitative and quantitative analysis of the results reveals that nonlinear black-box models based on system identification theory can successfully capture the nonlinearity of the SAGD process. Also, both workflows can control the subcool above the desired set-point while ensuring stable well operations. More than a 24% increment is achieved in net present value (NPV) using proposed NMPC workflows compared with the field operations with no closed-loop control. Overall, NMPC can successfully be used for improved RTO, energy efficiency, and greenhouse gas emissions while considering available surface facilities and well configurations.
Insight into the PZA resistance whole genome of Mycobacterium tuberculosis isolates from Khyber Pakhtunkhwa, Pakistan
Authors: Khan Muhammad Tahir , Ali Sajid , Khan anwar Sheed , Ali Arif , Khan Abbas , Kaushak Aman Chandra , Irfan Muhammad , Chinnasamy Satheshkumar , Zhang Shulin , Zhang Yu-Juan , Cui Zhelie , Wei Amie , Wang Yanjie , Zhao Mingzhu , Liu Kejia , Zeb Muhammad Tariq , wang Heng , Wei Dong Qing 
Published: June 2020

Abstract
Background

Tuberculosis (TB) is a global public health issue, getting worse due to emergence of resistance. Pyrazinamide (PZA) is first-line antimicrobial drugs used against non-replicated Mycobacterium tuberculosis (MTB). Data is scarce about whole genome sequencing of PZA resistance (PZA-R) in Khyber Pakhtunkhwa (KP) province of high burden country, Pakistan. In the current study we aimed to find the most common mutations in PZA-R MTB isolates in association with other candidate genes in a whole genome sequence (WGS). Samples were collected from TB suspects and drug susceptibility testing (DST) was performed according according to the WHO standards. The resistant samples were subjected for whole genome sequencing (WGS). The sequence data was through MTBseq and Total Genotyping Solution for Mycobacterium tuberculosis (TGS-TB). Metabolic model was analyzed, using RAST server.
Results

Among the three whole genome sequences, (NCBI BioProject Accession: PRJNA629298, PRJNA629388) 1997, 1162, and 2053 mutations including indel, was detected. Diverse variability has been detected in the membrane proteins PE and PPE, modulating the host immune response. Nine mutations in coding and promotor region have been detected in pncA with one novel (T-4C) variants. Mutations in the other drug candidate genes, KatG, rpoB have also been detected.
Conclusion

The metabolic model shows a distinct property. Diversity of variants has been detected in majority of MTB essential genes, functions from cell growth to cell signaling. The current study provides useful information, associated with geographic specific strains for biomarkers development and better management of drug resistance isolates.
Uterine Evacuation before Operative Hysteroscopy in Patients with Active Uterine Bleeding: A randomized controlled trial
Authors: Sayyah-Melli Manizheh , Kazemi-Shishavan Maryam , Behravan Nooshin , Gharabaghi Parvin Mostafa , Rahmani Vahideh 
Published: June 2020

Abstract
BackgroundConcurrent bleeding or existing clots usually obscure the vision field and decrease the success rate in hysteroscopy. Therefore, any effort to have a clear view during the hysteroscopy will meliorate the diagnostic or treatment outcomes. We examined the effect of preoperative clot evacuation on hysteroscopy and patient outcomes.MethodsIn this parallel-group trial, 114 patients with uterine bleeding were randomly assigned to receive either clot evacuation before standard operative hysteroscopy or standard hysteroscopy alone. The primary endpoints were the clarity of vision, amount of bleeding, the volume of consumed media, duration of the procedure, and postoperative complications.ResultsAll 114 participants completed the study. There were statistically significant differences in the frequency of the clear vision (p < 0.001), the severity of bleeding (p = 0.0006), mean procedure time (p < 0.001), mean serum volume used (p < 0.001), and the postoperative hematocrit levels [95% CI (-3.00, -1.88)] (p < 0.001) between groups. There was no difference related to in-hospital stay (p = 0.081) and anesthetic complications among the patients (p = 0.182). The procedure was successfully performed on all patients of both groups with zero postoperative complications.ConclusionRemoval of clots and other uterine contents before the insertion of the hysteroscope rendered better and faster access to the uterine wall to observe existing abnormalities. This method of starting the hysteroscopy procedure has a significant impact on surgical outcomes.Trial registration: Clinical trial registry name: Iranian Registry of Clinical TrialsUrl: https://en.irct.ir/trial/33369The registration number: IRCT20101130005283N13Date of registration: 2018-11-16
Silver Nanoparticles Offer a Synergistic Effect with Fluconazole Against Fluconazole-Resistant Candida Albicans by Abrogating Drug Efflux Bumps and Increasing Endogenous ROS
Authors: Sun Wenping , Jia Dongmei 
Published: June 2020

Abstract
Objectives: A frequent emergence of drug resistance has been observed and posed great threat to global public health recently. This work aimed to investigate the potential synergistic effect and the underlying mechanisms of AgNPs-fluconazole combination more extensively through 2 clinically isolated fluconazole-resistant Candida albicans (C. albicans) strains.Methods: Antifungal properties of AgNPs and fluconazole alone or together against planktonic cells and biofilms were tested. Cellular and molecular targets associated with fluconazole resistance were monitored after AgNPs treatment. Antifungal potential of AgNPs-fluconazole combination was also explored in vivo using a mouse model of disseminated candidiasis. Tissue burden and survival rate were analyzed. Results: The results indicated that AgNPs worked synergistically with fluconazole against both planktonic cells of fluconazole-resistant C. albicans and biofilms formed < 12 hours. AgNPs treatment down-regulated ERG1, ERG11, ERG25, and CDR2, decreased membrane ergosterol levels and membrane fluidity, reduced membrane content of Cdr1p, Cdr2p, and thus efflux bump activity. The elevated ROS production was also a likely cause of the synergistic effect. In vivo, AgNPs and fluconazole combination significantly decreased the fungal burden and improved the survival rate of infected mice. Conclusion: In conclusion, these results further confirm that AgNPs-fluconazole combination is a hopeful strategy for the treatment of fluconazole-resistant fungal infections.
Diarrhoeal Diseases in Children under five years Exhibits space-time disparities and priority areas for control interventions in Rwanda
Authors: NSHIMIYIMANA Ladislas , Onyambu Peris Monchari , Rutayisire Erigene 
Published: June 2020

Abstract
Background: The Diarrhoeal diseases remain to be a public health concern despite the existence of preventive measures and developing are the most affected. It affects more children under five years compared to the rest of the population. The burden of childhood diarrhoea varies with geographical area and time bound. A part from this variation, the link between climate change and diarrhoea among children under five years has not been well understood. This study aims to determine the trends, spatial temporal and seasonal characteristics of diarrhoea diseases among Rwandan children under five years using routine Health Management Information System (HMIS) data from 2014 to 2018.Methods: This was an ecological study using retrospective data analysis. Incidence were used to examine the trend, and excess hazard was assessed to determine the risk and likelihood for the occurrence of cases. Retrospective Discrete Poisson model was used to identify spatial, temporal and spatial-temporal clusters of diarrhoea. Linear regression was used to assess relationship between climatology variables and the occurrence of diarrhoea. Results: In total, 1,012,827 new diarrhoeal diseases episodes were reported during 2014-2018 with an annual incidence rate of 12,669/100,000 children under five years. Excess risk was noticed in 12/30 (40%) of country’s districts (RR>1). Most significant spatial clusters of diarrhoea were observed in the northern province (RR=1.66, p<001). We found a statistically significant positive and negative relationship between diarrhoeal disease, and temperature and rainfall respectively (p<0.001). Increase in one millimeter of rainfall was associated with decrease of 14 cases of diarrhoea while increase of one degree Celsius of temperature was associated with an increase of 17 diarrhoea cases.Conclusion: More districts were remarked with risk of diarrhoea which require targeted control intervention. Furthermore, significant association between diarrhoea case and climate dynamics was observed. This call for the public attention to climate changes which affect health especially children aged less than five years
Incidence and risk factors for postictal delirium in patients after electroconvulsive therapy in China
Authors: Cheng Nan , Liu Dezhao , Guo Yue , Du Jingyi , Xiang Ping , Hei Ziqing , Li Xiang 
Published: June 2020

Abstract
Aim: This study aimed to elucidate the incidence and risk factors for postictal delirium (PID) among Chinese patients undergoing electroconvulsive therapy (ECT). Methods: In this retrospective study, 203 patients who underwent ECT in the Third Affiliated Hospital of Sun Yat-Sen University from July 2016 to July 2017 had their PID severity measured by a previously developed scale. For data analysis, two groups were created: PID patients and non-PID patients. The groups were analyzed based on three types of independent variables: patient-related, electroconvulsive treatment, and hemodynamic variables. Data analysis was performed through descriptive statistics, Chi-squared tests, Fisher exact tests, and/or independent sampled t-tests. Logistic regression analysis was used to identify independent risk factors for postictal delirium (P < 0.05).Results: Results showed that 81 patients (39.9%) developed moderate to severe PID in their first ECT session. Patients receiving ECT for the first time (P = 0.016), agitation before the ECT (P = 0.028), and high heart rate variances (P = 0.044) were identified as risk factors for PID, and they were significantly correlated with the occurrence of moderate to severe PID (P < 0.01). Conclusion: The patients receiving ECT for the first time, with agitation states before ECT and/or with high heart rate variance during ECT procedures might be at higher risk for moderate to severe PID. The medical staff related to this type of treatment may benefit from detailed knowledge about the aforementioned risk factors for predicting PID and to anticipate the best possible management for these patients.
Protein convertase subtilisin/Kexin type 9 inhibits hepatocellular carcinoma growth by interacting with GSTP1 and suppressing the JNK signaling pathway
Authors: He Mingyan , Hu Jing , Fang Tingting , Tang Wenqing , Lv Bei , Yang Biwei , Xia Jinglin 
Published: June 2020

Abstract
Background PCSK9 has been found to be closely related to the occurrence and development of a variety of tumors. However, the concrete role of PCSK9 and its relationship with HCC development is largely unknown. Methods The expression levels of PCSK9 in HCC tissues and HCC cell lines were determined by quantitative real-time polymerase chain reaction (qRT-PCR), western blot and immunohistochemistry assays. The effects of PCSK9 expression on HCC biological traits were investigated by overexpressing and downregulating PCSK9 protein in vivo and in vitro. The mechanism by which PCSK9 mediates the depolymerization of the GSTP1 dimer and the phosphorylation of the JNK signaling pathway was further investigated. Results PCSK9 is downregulated in human HCC tissues. HCC cell proliferation, cell cycle phase distribution and apoptosis were inhibited by PCSK9 in vitro. PCSK9 suppresses tumor growth and metastasis of HCC in vivo. PCSK9 interacts with GSTP1 and promotes the depolymerization of the GSTP1 dimer and inactivation of the JNK signaling pathway. Furthermore, low PCSK9 protein expression in primary HCC tissues correlated with worse clinical outcomes. Conclusions PCSK9 inhibits HCC cell proliferation, cell cycle phase distribution and apoptosis by interacting with GSTP1 and suppressing the JNK signaling pathway, suggesting that PCSK9 might act as a tumor suppressor and may serve as a therapeutic target for the treatment of HCC.
Role of Pv-aCO2 gradient and Pv-aCO2/Ca-vO2 ratio during Cardiac Surgery
Authors: Bouchacourt Juan Pablo , Hurtado Francisco Javier , Kohn Eduardo , Illescas Laura , Dubin Arnaldo , Riva Juan 
Published: June 2020

Abstract
Background. Arterial lactate, mixed venous O2 saturation and parameters derived from CO2 metabolism as the venous minus arterial CO2 partial pressure (Pv-aCO2) gradient and the ratio between this gradient and the arterial minus venous oxygen content (Pv-aCO2/Ca-vO2) were proposed as markers of tissue hypoperfusion and oxygenation. The main goals were to characterize their physiologic determinants of Pv-aCO2 difference, and the Pv-aCO2 /Ca-vO2 ratio and the interchangeability of the variables calculated from mixed and central venous samples. Methods. We made a sub-analysis of 35 cardiac surgery patients included in a previous investigation database. Parameters were measured or calculated: after anesthesia induction (T1), end of cardiac surgery (T2), and at 6-8 hours intervals after ICU admission (T3 and T4). Results. Macrohemodynamics was characterized by increased cardiac index and low systemic vascular resistances, after surgery (p<0.05). Hemoglobin, arterial pH, lactate, and systemic O2 metabolism showed significant but transient changes during the study (p<0.05). Pv-aCO2 remained high and without changes along the study, and Pv-aCO2/Ca-vO2 was also high and only decreased at T4 (p<0.05). A weak but significant correlation was observed both, globally and at each time interval, between Pv-aCO2 or Pv-aCO2/Ca-vO2 with factors that may affect the CO2 hemoglobin dissociation. Using a similar approach, a multilevel linear regression model with Pv-aCO2 and Pv-aCO2/Ca-vO2 as outcome variables showed a significant association for Pv-aCO2 with mixed venous O2 saturation (SvO2), and base excess (BE) (p<0.05), while Pv-aCO2/Ca-vO2 was significantly associated with Hb, SvO2 , and BE (p<0.05) but not with cardiac output. Measurements and calculations from mixed and central venous blood were not interchangeable. Conclusions. Since Pv-aCO2 and Pv-aCO2/Ca-vO2 could be influenced by different factors that affect the CO2 dissociation curve, these variables should be considered with caution during the hemodynamic management of cardiac surgery patients. Finally, central venous and mixed values were not interchangeable.