bluuebunny/crossref_metadata_2025_split huggingface dataset:
292 The effect of pharmacological zinc on oral Salmonella vaccine efficacy
Authors: DeMille Carson M , Burrough Eric R , Gabler Nicholas 
Published: November 2020

Abstract
Pharmacological zinc (2,000-3,000 ppm) is commonly fed to nursery pigs to improve health and growth due to its antimicrobial and anti-inflammatory properties. The objective was to test if pharmacological zinc at time of oral Salmonella vaccination impeded vaccine efficacy. Sixty-four weaned pigs (5.1±0.7 kg BW) were used in a 2 x 2 factorial design. The diets were control (CON) or zinc (3,000 ppm for 1 week, 2,000 ppm for 2 weeks, and no additional zinc for 1 week [ZN]). On d 2 pigs were orally vaccinated for Salmonella with 1 of 2 commercially available vaccines, resulting in 4 treatments (CON1, CON2, ZN1, ZN2; n = 16/treatment). On d 28, n = 8 pigs/treatment were randomly selected and enrolled in a S. Typhimurium challenge study. On d 35 post-weaned, all pigs were inoculated with 108 cfu of a field S. Typhimurium isolate. Pig performance, febrile response, fecal shedding and serology was assessed over a 7-d challenge period. On dpi 7 all pigs were euthanized, and colon contents and ileocecal lymph nodes were collected for culture. The effect of nursery diet, vaccine and their interaction was assessed. Pigs were confirmed Salmonella culture positive at dpi 2 and 6 pigs were culture positive from the ileocecal lymph nodes at dpi 7. Salmonella-specific antibody titers (S/P) increased (P &lt; 0.001) from dpi 0 (0.31) to 7 (2.01), and a time-by-vaccine interaction was reported (P &lt; 0.05). Irrespective of diet and vaccine, core temperatures increased from 39.5°C (dpi 0) to 39.7°C (dpi 2) before decreasing (P = 0.02). Over the challenge period, ADG did not differ (0.67, 0.64, 0.61, 0.62 kg/d, CON1, CON2, ZN1, ZN2, respectively, P = 0.654). Furthermore, ADFI and G:F did not differ by diet or vaccine (P &gt;0.05). In conclusion, pharmacological Zn did not inhibit efficacy of oral Salmonella vaccines.
27 Shotgun proteomics reveal seminal plasma proteomes are reflective of boar reproductive performance
Authors: Mills Kayla M , Aryal Uma , Sobreira Tiago , Minton Amanda , Casey Theresa , Stewart Kara R 
Published: November 2020

Abstract
Subfertile boars have a negative economic impact for producers and need to be identified before they enter the breeding herd. Current semen evaluation techniques are not able to identify all subfertile boars. Seminal plasma proteins are essential for normal sperm function and transport and play an important role in fertilization. We hypothesize that seminal plasma proteins affect fertility, and thus differences in protein profiles reflect differences in boar fertility. The objective of this study was to use LC-MS/MS for shotgun proteome analysis to investigate whether differences in boar fertility phenotype can be differentiated by seminal plasma protein expression. Following 50 single-sire matings, boars were categorized into one of four phenotypes: high farrowing rate and high total born (HFHB; n=9), high farrowing rate with low total born (HFLB; n=10), low farrowing rate and low total born (LFLB; n=9), and low farrowing rate with high total born (LFHB; n=4). There were 436 proteins measured in at least one sample across all animals. There were 245 high confidence proteins and 56 were differentially abundant (P &lt; 0.05) between the high fertility phenotype (HFHB) and at least one of the three subfertile groups. Proteins associated with fertility and free radical detoxification were more abundant in HFHB whereas subfertile phenotypes had a greater abundance of blood microparticle proteins, biomarkers of inflammation, and IMPA1, which regulates inositol production. Findings support that seminal plasma protein profiles are distinct between boars with different fertility phenotypes and have the potential to predict boar reproductive performance.
1 Alternative heifer development systems utilizing corn residue and cover crops
Authors: Speer Hannah , Riley Hannah , Cushman Robert , Freetly Harvey , Drewnoski Mary 
Published: November 2020

Abstract
Spring-born heifers (n = 1,012) weaned at 148 ± 17 d were used in a 3-yr study to evaluate performance in winter development systems which utilized cover crop and corn residue grazing. Heifers were assigned to 1 of 3 treatments: grazing corn residue with dried distillers grains (CD) or wheat midds (CW) supplementation, or grazing late summer planted oat-brassica cover crop followed by corn residue supplemented dried distillers grains (CC). Grazing of corn residue (CD and CW) and cover crop (CC) began in early November. Supplementation during the corn residue phase was adjusted to target ~55% of mature BW (338 kg) at breeding. After 63 d, CC were moved to corn residue; on d 77 CD and CW began receiving grower ration. In mid-February (d 98), heifers were comingled and managed in a single group. Breeding season began in June and lasted for 29 d. Prior to corn residue grazing, ADG of CC was greater (0.76 kg/d; P&lt; 0.01) than CD or CW (0.58 kg/d and 0.49 kg/d, respectively). Gain during the last 35 d of the winter period for CC was 0.13 kg/d less than CW (P&lt; 0.01) but not different from CD. Overall winter ADG was greater (P&lt; 0.05) for CC (0.62 kg/d) than CD (0.53 kg/d) or CW (0.50 kg/d). Percent of mature BW prior to breeding was 52% for CC and 50% for CD and CW. May reproductive tract scores did not differ (P=0.26) between CC and CW but were greater (P&lt; 0.05) in CC than CD. Pregnancy rates were affected by treatment (P&lt; 0.01), with CC (76%) being greater than CD (68%) and CW (64%). Utilizing oat-brassica cover crops early in the winter followed by a lower rate of gain while grazing corn residue appear to be effective for developing beef heifers. USDA is an equal opportunity employer and provider.
106 Effects of Revalor-XH and combination Revalor-IH/Revalor-200 on Yearling Heifer Growth Performance and Carcass Characteristics
Authors: Carlson Zachary , Erickson Galen E , Dicke Bill , Streeter Marshall 
Published: November 2020

Abstract
A commercial feedlot trial utilizing 870 crossbred heifers (initial BW = 322; SD = 9 kg) examined two implant strategies on growth performance and carcass characteristics. Treatments included: 1) Revalor-IH on d 1 (80 mg trenbalone acetate (TBA)/8 mg estradiol (E2), noncoated) and re-implanted with Revalor-200 on d 101 (200 mg TBA/20 mg E2, noncoated (REV-IH/200); or 2) Revalor-XH on d 1 (200 mg TBA/20 mg E2, partially coated (REV-XH). There were no differences (P &gt; 0.23) observed for live performance including final BW, DMI, ADG, and feed efficiency between implant treatments. There were no differences (P ≥ 0.17) in carcass-adjusted final BW or ADG between implant strategies. There were no differences (P ≥ 0.28) observed for DMI before or after re-implanting for heifers given REV-IH/200 compared to heifers given REV-XH. Heifers implanted with REV-IH/200 had greater carcass-adjusted feed efficiency compared to REV-XH (0.156 vs. 152; P = 0.03). Heifers implanted with REV-IH/200 had larger LM area compared to heifers with REV-XH (88.4 vs. 83.9 cm2; P &lt; 0.01). Calculated yield grade was lower for heifers implanted with REV-IH/200 compared to heifers implanted with REV-XH (3.75 vs. 3.89; P &lt; 0.01). Hot carcass weights, dressing percent, marbling, and 12th rib back fat were not different (P ≥ 0.11) among treatments. Heifers implanted with REV-IH/200 had a shift towards lower USDA yield grade distribution compared to REV-XH implanted heifers (P = 0.05). The REV-IH/200 combination provided 280 mg of TBA and 28 mg E2, while total active ingredient dose for REV-XH was 200 mg TBA and 20 mg E2. Therefore, minimal differences were observed in carcass-adjusted feed efficiency, LM area, and calculated yield grade between REV-IH/200 and the partially coated REV-XH implant.
226 Influence of trace mineral source on digestion, ruminal volatile fatty acid and soluble mineral on steers fed a dairy type diet balanced to meet requirements for a high producing lactating dairy cow
Authors: Guimaraes Octavio , Wagner John , Spears Jerry , Engle Terry 
Published: November 2020

Abstract
Twelve Angus steers (BW 530.9 ± 22.7kg) fitted with ruminal cannulae were used to determine the impact of trace mineral source on digestibility, ruminal VFA, and soluble concentrations of Cu, Zn, and Mn. Steers were fed a dairy type diet balanced to meet requirements for a high producing lactating dairy cow (DM basis: 16.8% CP, 29.2 Mcal NEm, 30.3 Mcal NEl, 33.6% NDF) for 21 d. Treatments consisted of 10 mg Cu, 40 mg Mn, and 60 mg Zn/kg DM from either sulfate (STM), hydroxy (HTM; IntelliBond) or organic (ORG; metal specific amino acid complexes) sources (n=4 steers/treatment). Following a 21-d adaptation period, total fecal output was collected for 5 d. On d 6, rumen fluid was collected at 0, 2, and 4 h post feeding and analyzed for VFA. Steers were then fed the same diet without supplemental Cu, Zn, or Mn for 14 d. On d 15 steers received a pulse dose (cannulae) of 100 mg Cu, 400 mg Mn, and 60 mg Zn from either STM, HTM, or ORG. Ruminal samples were obtained at 2-h intervals starting at -4 and ending at 24 h post dosing and analyzed for soluble Cu, Mn, and Zn. Digestibility of NDF and ADF were lesser (P &lt; 0.05) in STM vs. HTM and ORG supplemented steers. Steers receiving HTM and ORG had greater (P &lt; 0.05) total VFA concentrations than STM supplemented steers at 2 and 4h post feeding. Ruminal soluble Cu and Zn concentrations were greater (P &lt; 0.001) post dosing in STM and ORG supplemented steers at 2, 4, and 6 h for Cu and 4, 6, 8, 10 and 12 h for Zn when compared to HTM supplemented steers. Results indicate that ruminal solubility of Cu and Zn differs between STM, HTM and ORG sources and trace mineral source impacts fiber digestion and ruminal VFA concentrations.
247 The effect of diet on the microbial community structure and composition in lactating Jersey cows consuming a mixture of straw and dry distillers grains plus solubles in replacement of alfalfa hay
Authors: Knoell Allison , Aluthge Nirosh , Abbas Waseem , Bartenslager Alison , Judy Jared , Morris Dennis , Wilson Hannah C , Herrick Kevin , Kononoff Paul J , Fernando Samodha 
Published: November 2020

Abstract
The rumen microbial community is responsible for producing a majority of the energetic needs for the animal, yet our understanding of the rumen microbiome is in its infancy. To better understand the effect of corn-ethanol coproducts on rumen microbial communities, a replicated 4 × 4 Latin square design study utilizing 12 cows in three squares was conducted to evaluate the replacement of alfalfa hay with a mixture (CoP) containing straw and dried distillers grains plus solubles (DDGS) in lactating Jersey cows. The experimental treatments were (proportions on a dry matter basis): a control diet (CON) containing 18.2% of alfalfa hay with no straw or DDGS. A low coproduct diet (LCoP) containing 12.1% alfalfa, 2.1% straw, and 6.0% DDGS. A medium coproduct diet (MCoP) containing 6.1% alfalfa, 4.2% straw, and 12.1% DDGS. A high coproduct diet (HCoP) containing 6.2% straw, 18.1% DDGS with no alfalfa. Rumen digesta samples were collected via an esophageal tube. No differences were observed for milk production and dry matter intake (P ≥ 0.307) (mean ± SEM) 19.5 kg ± 0.60, 29.6 kg ± 0.91, across treatments, while a decrease in methane was observed (P &lt; 0.01) for the HCoP treatment. The bacterial community was assessed by sequencing the V4 region of the 16S rRNA gene. Additionally, the archaeal community was assessed by sequencing the V4-V5 region of the 16S rRNA gene on the Illumina MiSeq platform. Amplicon Sequence Variants were identified using the DADA2 pipeline. No significant differences were observed for the bacterial (P = 0.334) and archaeal (P = 0.593) communities. Although global effects in microbial community dynamics were not observed, differential taxa were observed with Lachnospiraceae being the major differentially abundant Family. The archaeal community composition demonstrated that Methanobacteriales to be the differentially abundant Order across treatments, and may contribute to methane production.
70 Identifying factors that play a significant role in the animal science transfer student experience
Authors: Bundy Jennifer , Siberski Cori 
Published: November 2020

Abstract
Transfer students make up 16.5% of the undergraduate population in the Animal Science (ANS) major, and transfer enrollment is on the rise. Transfers often face challenges that are different from their direct-from-high-school peers. The objective of this study was to determine the factors that affected the transfer transition from a previous institution to the ANS department. Based on focus group (n=6) feedback, a survey instrument was developed and piloted. Using pilot data, a factor analysis was performed and the instrument was tested for reliability. Once validated, the instrument was used to collect data from first semester ANS transfer students (n=47). No incentives were offered, and participation was voluntary. Between-factor Pearson correlations were estimated, and responses to scale questions were tallied. Significant negative correlations were observed between social integration and risk of attrition (r=-0.53, p&lt; 0.01), and between social integration and academic struggle (r=-0.48, p&lt; 0.01). Social integration was positively correlated with overall satisfaction within the ANS department (r=0.638, p&lt; 0.01). Students who felt socially integrated were more confident academically, more comfortable at ISU, and were less likely to leave. Students had a positive view of the ANS department if they were involved in clubs and student organizations (r=0.42, p&lt; 0.05). Transfers who were satisfied with instructor interactions tended to be more content with the overall transfer process (r=0.44, p&lt; 0.01). Approximately 72% of respondents felt that paying for school created a lot stress in their life, but 52% felt satisfied with the financial assistance they received. Only half of the respondents reported that the pre-registration orientation helped prepare them for transfer to ISU, but 95.9% of ANS transfers reported that they understood the requirements for graduation. These data will inform policy and procedures related to transfer student onboarding and the promotion of social interactions with peers.
71 Imitating the dynamic bovine cervix with 3D printing technology to teach artificial insemination in cattle
Authors: McCabe Esther D , Sorell Lane P , Zhang Meng , Hartman Ashley , Fike Karol E , Odde Kenneth G , Grieger David M 
Published: November 2020

Abstract
The process of artificial insemination in cattle is commonly taught using excised reproductive tracts. Excised reproductive tracts can be difficult to collect, and often require freezing and thawing prior to use; however, they are an effective tool to teach artificial insemination (AI) techniques. As an alternative tool, 3D printed cervixes were created using NinjaFlex filament in a Flashforge Creator Pro Dual Extrusion Printer. Designs of cervixes were created with a 3D CAD software while MATLAB was used to generate the random placement of the cervical ring openings to model biological diversity. The objective was to determine the effectiveness of using a 3D printed cervix compared with an excised reproductive tract to teach AI in cattle. Data were collected via surveys for 120 students from 2016 through 2019. Students were divided in two groups prior to entering live animals: 1) Excised reproductive tract and 2) 3D Cervix. The excised reproductive tracts were previously collected, preserved, and confirmed passable by instructors. The 3D cervix group included 3D cervixes mounted in a bovine pelvis inside a box. Overall, 88% of students had success passing the cervix in the cow by the third day. Eighty-seven percent of students thought the excised tracts felt somewhat similar to a live cow, compared with 54% who thought the 3D cervixes alone felt similar to a live cow. When the 3D cervixes were mounted in the box, 17/20 students thought they felt similar to a live cow. For learning AI techniques, 12/21 students thought the excised tracts were more helpful than the 3D cervixes alone (5/21); however, when the 3D cervixes were mounted in the pelvic boxes, 11/21 students preferred this setup compared with the excised tracts (6/21 students). Student feedback suggests the 3D cervixes mounted in pelvic boxes are a useful tool for teaching artificial insemination techniques.
PSV-5 Effect of oxygen administration to piglets at birth on post-natal body temperatures
Authors: Cooper Naomi , Vande Pol Katherine , Ellis Michael , Xiong Yijie , Gates Richard 
Published: November 2020

Abstract
Newborn piglets are susceptible to hypothermia, and associated mortality. Previous research suggested exposing neo-natal piglets to elevated oxygen concentrations reduced post-natal body temperature decline. This study evaluated the effects of oxygen administration at birth on piglet temperatures over 24 h after birth. This study used 296 piglets (24 litters) and was carried out in a standard farrowing facility consisting of crates within pens with a heat lamp. A GRBD was used; sow was the blocking factor; piglet the experimental unit. A replicate was 3 littermates of similar BW; piglets were randomly allotted at birth within replicate to treatment: 1) Control [dried at birth (with a desiccant); returned to farrowing pen]; 2) Ambient Oxygen (dried; placed in a chamber at ambient temperature and oxygen concentration for 20 min); 3) 40% Oxygen (dried; placed in a chamber at ambient temperature and 40% oxygen concentration for 20 min). Piglets were weighed at birth. Rectal temperature was measured at 0, 20, 30, 45, 60, 90, 120, 180, 240 and 1440 min after birth. Data were analyzed using PROC MIXED of SAS. The model included fixed effects of treatment, time of measurement (repeated measure), and the interaction and random effects of block and replicate. There was no treatment effect (P &gt; 0.05) on temperatures at birth and between 120 and 1440 min. Compared to the Control, temperatures were lower (P &lt; 0.05) for the 40% Oxygen treatment between 20 and 90 min and for the Ambient Oxygen treatment between 20 and 45 min. Temperatures at 60 and 90 min for the Ambient Oxygen treatment were intermediate to and not different (P &gt; 0.05) from the other treatments. Results suggest that exposing neo-natal piglets to an oxygen-rich environment in a chamber does not reduce post-natal temperature decline. This research was funded by the National Pork Board.
PSV-2 An exploration of sociality among pre-weaned beef calves
Authors: Robbins Jesse , Parsons Rebecca , Dewell Grant , Haley Derek , Johnson Anna , Dewell Reneé , Millman Suzanne 
Published: November 2020

Abstract
Preferential relationships have been reported between individual cows within a herd. However, less is known about the ontogeny of preferential relationships between calves and subsequent bonding behavior. The objective of this study was to characterize these relationships in pre-weaned beef calves, with the hypothesis that calves form preferential relationships with one another prior to weaning. Ten weeks prior to commencing formal observations, forty mature Angus cows and their calves were randomly selected from a research herd, balanced for calf sex and allocated to one of two grazing paddocks approximately 12 acres in size. Two weeks prior to weaning, direct observations of calf behavior were performed over period of 12 days, using instantaneous scan sampling between 0700-1230h or 1300-1830h, resulting in 6 scans per calf/day X 12 days (72 scans/calf). For each calf, the identity and proximity (in calf lengths) of the calf in closest proximity (aka nearest neighbor) was noted, as well as behavior. The ethogram included calf behavior (grazing, nursing, lying, drinking, locomotion, social contact and standing idle), and sociality was categorized as being within 5 calf lengths of another calf. Sociality was observed in 60% of all scans, and sociality of individual calves ranged from 47% to 72% of the scans observed (72 scans/calf). The statistical model investigating sociality included calf sex and the interaction of day and paddock. Sociality was equally likely in bulls and heifers (0.59 +/- 0.1; p=0.80). A significant day by paddock interaction was also identified (p=0.001), but no obvious trends were apparent. In conclusion, preliminary results provide support for individual differences in sociality of pre-weaned beef calves prior to weaning. Further analysis will explore if preferential relationships exist between individual calves, and how these might be associated with different behavioral states.
PSV-3 Detection of Bruising in Holstein Slaughter Cows with Infrared Thermography
Authors: Kirk Ashlynn , Vogel Kurt 
Published: November 2020

Abstract
Identifying severe bruising in livestock before slaughter is valuable because bruises are likely a source of pain for the animal that cannot be seen by visual inspection. This bruising may result in major trim losses because bruised tissue is considered unfit for human consumption. The objective of this study was to determine if bruising could be detected under the hide of live Holstein cull cows with infrared thermal imaging technology. A FLIR E8 thermal imaging camera was utilized to capture heat emission images pre-hide removal and post hide removal for 36 Holstein cull cows. Images were collected approximately 2 m from each cow and associated carcass images were examined on FLIR Tools software to determine if and where inflammation was detected on the carcass. Trimming occurs throughout the slaughter process, but carcasses may be ‘railed out’ if additional trimming is necessary. Trim loss was collected from railed out carcasses and weighed. The impact of rail-out status on trim loss was significant (P &lt; 0.0001). Carcasses that were railed out (n = 15) yielded 16.2 ± 2.0 kg of trim loss. Carcasses that were not railed out (n = 21) did not experience additional trim loss. Carcasses railed out for additional trimming where inflammation suggested bruising existed (n = 7) yielded 21.0 ± 4.8 kg of trim loss. Carcasses railed out for trimming where thermography did not detect bruising (n = 4) yielded 12.6 ± 6.4 kg of trim loss. The relationship between thermographic detection of bruises and trim loss among railed-out carcasses was not significant (P = 0.3200). Inflammation that predicted rail-out events was 63.64% while carcasses in which rail-out was not detected by thermography was 36.36%. It appears that infrared thermography has potential to identify carcass bruising but additional investigation is warranted to understand its limitations.