bluuebunny/crossref_metadata_2025_split huggingface dataset:
Recombinant Adeno-Associated Viral Vectors Expressing Human Coagulation FIX-E456H Variant in Hemophilia B Mice
Authors: Le Quellec Sandra , Dane Allison P. , Barbon Elena , Bordet Jean-Claude , Mingozzi Federico , Dargaud Yesim , Marais Thibaut , Biferi Maria-Grazia , Négrier Claude , Nathawani Amit C. , Enjolras Nathalie 
Published: October 2019

AbstractGene therapy using recombinant adeno-associated virus (AAV) has induced sustained long-term coagulation human factor IX (hFIX) levels in hemophilia B (HB) patients. However, asymptomatic transient liver toxicity was observed at high vector doses, highlighting the need to improve the potency of these vectors. We report the generation of an AAV transgene cassette containing the hyperfunctional hFIX-E456H variant showing improved binding to platelets, with a comparison to wild-type hFIX (hFIX-WT) and hFIX-R384L variant (Padua) transgenes, containing F9 truncated-intron 1 (I1). In vitro specific activity was increased by 3.2- and 4.2-fold with hFIX-E456H and hFIX-R384L variants compared with hFIX-WT, using chromogenic assay, and by 7-and 8.6-fold with hFIX-E456H and hFIX-R384L variants compared with hFIX-WT, using one-stage assay. The transgenes were packaged into single-stranded AAV2/8 vectors that were tail vein injected at 5 × 109, 2 × 1010, and 5 × 1010 vg per mouse in HB mice. Plasma FIX activity level, assessed by chromogenic assay, was up to fourfold higher for hFIX-E456H compared with hFIX-WT and was not different compared with hFIX-R384L, among the three dose cohorts. Overall, the in vivo specific activity was increased by threefold for hFIX-E456H and 4.9-fold for hFIX-R384L compared with hFIX-WT. At the lower dose of 5 × 109 vg, the blood loss was significantly lower for hFIX-E456H compared with hFIX-WT, but did not differ compared with hFIX-R384L. The results found for the hFIX-E456H variant indicate that it might be a suitable alternative for gene therapy of HB.
Dietary Intake of Marine Polyunsaturated n-3 Fatty Acids and Risk of Recurrent Venous Thromboembolism
Authors: Isaksen Trond , Evensen Line H. , Brækkan Sigrid K. , Hansen John-Bjarne 
Published: October 2019

Abstract
Background Limited knowledge exists on the association between intake of long-chained n-3 polyunsaturated fatty acids (n-3 PUFAs) and risk of recurrence and all-cause mortality in patients with venous thromboembolism (VTE).
Objectives This article investigates whether intake of marine n-3 PUFAs was associated with risk of recurrence and mortality in patients with incident VTE.
Methods A total of 595 patients with incident VTE and available data on n-3 PUFA intake were derived from the Tromsø Study surveys 4 (1994–1995) and 6 (2007–2008). Weekly intake of n-3 PUFAs was categorized as low, medium, and high based on tertiles. Recurrent VTEs and all-cause mortality were registered up to December 31, 2016. Hazard ratios (HRs) were calculated using Cox regression models with the low intake category as reference.
Results There were 98 recurrent VTEs and 227 deaths during follow-up. Overall, we found no association between intake of n-3 PUFAs and risk of recurrent VTE. However, inverse associations were found for high intakes in patients with unprovoked VTE (HR 0.45, 95% confidence interval [CI]: 0.20–1.01), cancer-free patients (HR 0.51, 95% CI: 0.27–0.95), and deep vein thrombosis (DVT) patients (HR 0.49, 95% CI: 0.24–0.97). The inverse associations were more evident when follow-up was restricted to the time after discontinuation of anticoagulant therapy. No association was observed between intake of n-3 PUFAs and mortality after incident VTE.
Conclusion A high dietary intake of marine n-3 PUFAs was associated with lower risk of recurrent VTE after unprovoked index events, DVT, and in cancer-free patients.
Retinotopic specializations of cortical and thalamic inputs to area MT
Authors: Mundinano Inaki-Carril , Kwan William C. , Bourne James A. 
Published: October 2019

Significance
Foveal specializations in the primate visual system have allowed for high-acuity vision and are crucial to the evolution of the brain and primate behaviors. Here we show that the dorsal visual stream, which is dedicated to processing visual motion and visually guided behaviors, comprises foveal specializations. We report that spiny stellate cells, an anatomically specialized type of projection neuron, are distributed mainly in the foveal portion of the primary visual cortex (V1)-middle temporal area (MT) pathway. Moreover, we demonstrate that the thalamic input to the MT area from the medial inferior pulvinar is organized in a retinotopic manner. These results change our understanding of the anatomic organization of the early visual system.
Compact single-shot metalens depth sensors inspired by eyes of jumping spiders
Authors: Guo Qi , Shi Zhujun , Huang Yao-Wei , Alexander Emma , Qiu Cheng-Wei , Capasso Federico , Zickler Todd 
Published: October 2019

Significance
Nature provides diverse solutions to passive visual depth sensing. Evolution has produced vision systems that are highly specialized and efficient, delivering depth-perception capabilities that often surpass those of existing artificial depth sensors. Here, we learn from the eyes of jumping spiders and demonstrate a metalens depth sensor that shares the compactness and high computational efficiency of its biological counterpart. Our device combines multifunctional metalenses, ultrathin nanophotonic components that control light at a subwavelength scale, and efficient computations to measure depth from image defocus. Compared with previous passive artificial depth sensors, our bioinspired design is lightweight, single-shot, and requires a small amount of computation. The integration of nanophotonics and efficient computation establishes a paradigm for design in computational sensing.
Plant defense resistance in natural enemies of a specialist insect herbivore
Authors: Zhang Xi , van Doan Cong , Arce Carla C. M. , Hu Lingfei , Gruenig Sandra , Parisod Christian , Hibbard Bruce E. , Hervé Maxime R. , Nielson Chad , Robert Christelle A. M. , Machado Ricardo A. R. , Erb Matthias 
Published: October 2019

SignificanceCertain adapted insect herbivores utilize plant toxins for self-defense against their own enemies. These adaptations structure ecosystems and limit our capacity to use biological control agents to manage specialized agricultural pests. We show that entomopathogenic nematodes that are exposed to the western corn rootworm, an important agricultural pest that sequesters defense metabolites from maize, can evolve resistance to these defenses. Resisting the plant defense metabolites likely allows the nematodes to infect and kill the western corn rootworm more efficiently. These findings illustrate how predators can counter the plant-based resistance strategies of specialized insect herbivores. Breeding or engineering biological control agents that resist plant defense metabolites may improve their capacity to kill important agricultural pests such as the western corn rootworm.
Hybridization increases population variation during adaptive radiation
Authors: Grant Peter R. , Grant B. Rosemary 
Published: October 2019

SignificanceAdaptive radiations, comprising many species derived from one or a small number of ancestral species in a geologically short time, are prominent components of the world’s biodiversity. Introgressive hybridization of divergent species has been important in increasing variation, leading to new morphologies and even new species, but how that happens throughout evolutionary history is not known. A long-term field study of Darwin’s finches on Daphne Major island, Galápagos, shows that introgression enhances variation and increases the potential for future evolution. We use a dated phylogeny to infer that populations became more variable in morphological traits through time, consistent with this enhancement effect, and then declined in variation after reaching a maximum. Introgression may be especially important with future climate change.
How kinesin waits for ATP affects the nucleotide and load dependence of the stepping kinetics
Authors: Takaki Ryota , Mugnai Mauro L. , Goldtzvik Yonathan , Thirumalai D. 
Published: October 2019

Significance
Dimeric molecular motors walk on polar tracks by binding and hydrolyzing one ATP per step. Despite tremendous progress, the waiting state for ATP binding in kinesin that walks on microtubule (MT), remains controversial. One experiment suggests that in the waiting state both heads are bound to the MT, while the other shows that ATP binds to the leading head after the partner head detaches. To discriminate between these 2 scenarios, we developed a theory to calculate accurately several experimentally measurable quantities as a function of ATP concentration and resistive force. In particular, we predict that measurement of the randomness parameter could discriminate between the 2 scenarios for the waiting state of kinesin, thereby resolving this standing controversy.
“Brexit means…”
Authors: Tincheva Nelly 
Published: October 2019

AbstractBrexit, i.e. the withdrawal of theukfrom theeu, is an event, which, regardless of its course and destination, has had a lasting impact on international politics. This paper offers a cognitive linguistic perspective on Brexit and investigates its conceptual metaphorizations on the first days after theukreferendum. The paper compares data fromukand non-uk eumedia. The perspective adopted is that of Socio-Cognitive Studies in combination with quantitative analysis. The findings suggest there are significant differences betweenuksource domains and continentaleuones, e.g. overall metaphor use throughout the period is more pronounced in theuksub-dataset; in theuksub-dataset,divorcemetaphorizations characterize either a ‘break-up’ between ‘two Britains’ or anuk-eu‘break-up’;journey, disasterandwarare more frequently used in theuk, whilemechanical failureis more prominent in the continental sub-dataset. Overall, a preference for inanimate source domains characterizes both sub-datasets.
Antibiotics can cause weight loss by impairing gut microbiota in mice and the potent benefits of lactobacilli
Authors: Miao Zhonghua , Cheng Ruyue , Zhang Yujie , Liang Huijing , Jiang Fengling , Shen Xi , Chen Gong , Zhang Qisheng , He Fang , Li Ming 
Published: October 2019

ABSTRACT
This study assessed whether antibiotics could alter gut microbiota to affect host growth and the possibility of alleviation by lactobacilli. We divided four-week-old BABL/c mice into control (Ctrl), antibiotic exposure (Abx), Lactobacillus plantarum PC-170 (PC), and Lactobacillus rhamnosus GG (LGG) group and the Abx, LGG, and PC group received an one-week antibiotic/antibiotic + probiotic treatment. The fecal microbiota and the expression of splenic cytokines were determined. Following the ceftriaxone treatment, the body weight gain of Abx was delayed compared with others. The ceftriaxone treatment significantly decreased the alpha-diversity of the fecal microbiota and altered the fecal microbiota but LGG and PC can partly alleviate the effect. At the end of the study, the microbial community of LGG and PC group were more similar to Ctrl compared with Abx group. The results indicated that ceftriaxone could significantly alter intestinal microbiota. Lactobacilli might alleviate the side effects of antibiotics by stabilizing the intestinal microbiota.
Continuous-variable quantum computing in the quantum optical frequency comb
Authors: Pfister Olivier 
Published: October 2019

Abstract
This topical review introduces the theoretical and experimental advances in continuous-variable (CV)—i.e. qumode-based in lieu of qubit-based—large-scale, fault-tolerant quantum computing and quantum simulation. An introduction to the physics and mathematics of multipartite entangled CV cluster states is given, and their connection to experimental concepts is delineated. Paths toward fault tolerance are also presented. It is the hope of the author that this review attract more contributors to the field and promote its extension to the promising technology of integrated quantum photonics.
Glycerine as an electrolytic electrode
Authors: Sh Akhatov G , Kayumov R R , Valeeva R R , Akhatov M F 
Published: October 2019

Abstract
This paper presents the results of the experimental studies of an electric discharge with liquid electrodes. An aqueous solution of NaCl in glycerine was used as liquid electrodes, in the range of discharge voltage parameters Up = 0 ÷ 1.5 kV, currents I = 0.01 ÷ 2 A. The current-voltage characteristics of the discharge were established.
Electric properties of carbon films deposited by magnetron sputtering with ion assistance
Authors: Grushin M A , Kral’kina E A , Neklyudova P A , Nikonov A M 
Published: October 2019

Abstract
The paper deals with the study of ion assistance impact on structure and electrophysical properties of the carbon films deposited by magnetron sputtering of graphite targets. The flow of assisting ions was generated in the plasma reactor based on inductive RF discharge located in the external magnetic field. The change of the ionic current density in the range from 0.5 to 1 mA/cm2 was provided. Ion energy was changed by biasing the substrate in the range from 10 to 85 V. Neon, argon and krypton were used as working gases. The electrophysical properties of carbon films are shown to depend non-monotonous on energy of the assisting ions.
Internal characteristics of distribution of glow discharge at supersonic speed gas flow in the positive column area
Authors: Timerkaev B A , Zalyaliev B R , Egorov R E 
Published: October 2019

Abstract
A discharge device providing the ability to create an area in the interelectrode space with a controlled concentration of neutral particles due to the supersonic flow arrangement has been developed and implemented. The possibility to control the internal discharge parameters distribution by supersonic gas flow arrangement in a limited area of the glow discharge is shown experimentally.
The influence of the capacitive coupled radio-frequency discharge on cotton fiber and technological effects of its application
Authors: Borodaev I A , Azanova A A , Zheltukhin V S , Abutalipova L N , Khisamiyeva L G 
Published: October 2019

Abstract
The authors studied the effect of capacitively coupled radio-frequency (RF) discharge at low pressure on cotton fiber. It is shown that non-cellulose impurities of cotton fiber are removed during processing: the content of extracted substances is reduced by 22-70% depending on the type of solvent when using air, by 5-6% – when using argon. The surface of the fibers becomes hydrophilic, additional submicroscopic pores appear, the roughness increases 1.6 times, water absorption increases 1,5-1,8 times. Discontinuous knitted fabric dyeing was carried out varying the concentration of the active dyes (remasol) in the dye-bath. It was found that the sorption capacity of cotton fibers in relation to the dye increases on average 1.1-1.25 times. To achieve the required color intensity of plasma pre-treated samples dye concentration on average 10-30% less than in the controlled dyeing is needed. The concentrations of dyes that allow obtaining the same intensity of color for the knitted fabric prepared in different ways are selected.
Pulsating combustion of solid particles in Helmholtz resonator type device
Authors: Larionov V M , Iovleva O V , Zagidullina D I , Larionova I V 
Published: October 2019

Abstract
There are a number of works in which pulsating combustion of the wood particles in Helmholtz resonator type device was studied. The dependences of the frequency and amplitude of oscillations on the geometry of the device were determined. The aim of this work is to study the thermodynamic properties of pulsating combustion. P-V diagrams of pulsating combustion and the amplitude of oscillations of the heat release rate are determined. It is proved that the acoustic power generated in the combustion zone is the result of work performed on the gas during one oscillation cycle.
Acoustic-resonance method for control of the location of hidden hollow objects
Authors: Nazarychev S A , Gaponenko S O , Kondratiev A E , Shakurova R Z 
Published: October 2019

Abstract
There are quite a number of methods for monitoring the location of buried pipelines: radio wave method, radiation method, acoustic location, resonant-acoustic profiling, infrared thermography, electromagnetic method.
However, these methods have several disadvantages: they are not applicable to pipelines of non-metallic materials, and also have a weak selectivity in controlling the location of buried pipelines when many different communications pass in close proximity to the object under control.
In this work, an improved acoustic-resonance method for controlling the location of hidden hollow objects has been developed and implemented as an information-measuring complex consisting of devices for exciting and receiving forced vibrations. The algorithmic and software-technical support of the processes of controlling the information-measuring complex and processing of vibroacoustic signals in the LabVIEW environment has been developed and created.
Investigation of the plasma electron source based on discharge in crossed electric and magnetic field in hollow configuration
Authors: Podguyko N A , Marahtanov M K , Khohlov Yu A 
Published: October 2019

Abstract
This article presents a study of the plasma electron source based on discharge in crossed electric and magnetic fields in hollow configuration with a discharge current of up to 1 A. It was found that using a cathode screen with a central hole affects the operation of the device. The discharge voltage is reduced and the formation of an electron beam becomes possible without using for this purpose the extraction voltage. It is shown that the current strength of the electron beam in the absence of an extraction voltage depends on the flow rate of the plasma-forming gas.
Analysis of interaction processes between the silver nanoparticles and the surface of fur semi-finished products within conditions of HF plasma at decreased pressure
Authors: Pankova E A , Nizamova D K , Rakhmatullina G R , Shaekhov M F , Sharifullin F S 
Published: October 2019

Abstract
In order to impart the bactericidal properties and the additional strength to the fur semi-product, it is proposed to perform its treatment with the silver nanoparticles within conditions of high frequency (HF) plasma at decreased pressure.
Silver comes to the fur surface in atomic and ionized form, which significantly facilitates its interaction with keratin, provides their efficient adsorbing on the surface of fur semi-product and forming the stable compositions+.
Study of the possibility of obtaining metal nanoparticles under the action of a non-equilibrium plasma on aqueous solutions of salts
Authors: Titov V A , Sirotkin N A , Nikitin D I , Naumova I K , Subbotkina I N , Kochkina N E , Agafonov A V 
Published: October 2019

Abstract
Dispersions of metal nanoparticles were obtained at the gas discharge treatment of aqueous solutions of CuCl2, CuSO4 and AgNO3. Direct current discharge was excited between a metal cathode located in the air and metal salt solution being used as an anode at a current of 5–25 mA. The average sizes of obtained nanoparticles were estimated using dynamic light scattering method. The sizes have been shown to depend on the solution concentration, discharge current and processing time. The obtained nanoparticles were applied from dispersions to medical dressings and their bactericidal activity was tasted. Samples treated with silver-containing particles showed bactericidal activity against bacteria Escherichia coli, Staphylococcus albus, Bacillus subtilis and microscopic fungus Candida albicans. Copper-containing samples did not show biological activity.
Disentangling the impacts of human and environmental change on catchment response during Hurricane Harvey
Authors: Sebastian Antonia , Gori Avantika , Blessing Russell B , van der Wiel Karin , Bass Benjamin 
Published: October 2019

AbstractFlooding is a function of hydrologic, climatologic, and land use characteristics. However, the relative contribution of these factors to flood risk over the long-term is uncertain. In response to this knowledge gap, this study quantifies how urbanization and climatological trends influenced flooding in the greater Houston region during Hurricane Harvey. The region—characterized by extreme precipitation events, low topographic relief, and clay-dominated soils—is naturally flood prone, but it is also one of the fastest growing urban areas in the United States. This rapid growth has contributed to increased runoff volumes and rates in areas where anthropogenic climate changes has also been shown to be contributing to extreme precipitation. To disentangle the relative contributions of urban development and climatic changes on flooding during Hurricane Harvey, we simulate catchment response using a spatially-distributed hydrologic model under 1900 and 2017 conditions. This approach provides insight into how timing, volume, and peak discharge in response to Harvey-like events have evolved over more than a century. Results suggest that over the past century, urban development and climate change have had a large impact on peak discharge at stream gauges in the Houston region, where development alone has increased peak discharges by 54% (±28%) and climate change has increased peak discharge by about 20% (±3%). When combined, urban development and climate change nearly doubled peak discharge (84% ±35%) in the Houston area during Harvey compared to a similar event in 1900, suggesting that land use change has magnified the effects of climate change on catchment response. The findings support a precautionary approach to flood risk management that explicitly considers how current land use decisions may impact future conditions under varying climate trends, particularly in low-lying coastal cities.
Research on data system framework and key technologies of EV charging station maintenance
Authors: Liao Jiaqi , Fang Hualiang , Qian Kejun , Zhou Chengke , Dai Luyang 
Published: October 2019

Abstract
With the increase in the utilization rate of EV (Electric vehicle) charging facilities, the perception of operation status and the intelligent maintenance have become the key technologies of charging station development. A charging station data analysis method based on the two-dimension data framework is proposed according to the power transmission characteristic and data classification. Combined with the frequency and characteristic of fault events as well as the working principle of the charging station, the hardware and software framework based on the data processing and analysis for state evaluation and scientific maintenance planning is designed. Finally, the actual operation and maintenance statistics of the electric bus charging station in Suzhou, China is taken as an example for analysis.
Study of two-spring piezoelectric harvesters
Authors: Georgiev N P , Raichev R P 
Published: October 2019

Abstract
The paper considers two piezoelectric harvesters, consisting of two springs, attached to a piezoelectric transformer, and having a common concentrated mass. Modeling has been done by means of ANSYS, whereas the horizontal deviation of the mechanical system has been obtained, and the charge, accumulated on the electrodes of the piezo plate has been calculated. An equivalent circuit has been drawn and experimental studies have been conducted in order to validate the developed model.