bluuebunny/crossref_metadata_2025_split huggingface dataset:
Exploring optimal examination to detect occult anastomotic leakage after rectal resection in patients with diverting stoma
Authors: Kitaguchi Daichi , Enomoto Tsuyoshi , Ohara Yusuke , Owada Yohei , Hisakura Katsuji , Akashi Yoshimasa , Takahashi Kazuhiro , Ogawa Koichi , Shimomura Osamu , Oda Tatsuya 
Published: February 2020

Abstract
Background

When considering “early stoma closure”, both standardized inclusion/exclusion criteria and standardized methods to assess anastomosis are necessary to reduce the risk of occult anastomotic leakage (AL). However, in the immediate postoperative period, neither have the incidence and risk factors of occult AL in patients with diverting stoma (DS) been clarified nor have methods to assess anastomosis been standardized. The aim of this study was to elucidate the incidence and risk factors of occult AL in patients who had undergone rectal resection with DS and to evaluate the significance of computed tomography (CT) following water-soluble contrast enema (CE) to detect occult anastomotic leakage.

Methods

This was a single institutional prospective observational study of patients who had undergone rectal resection with the selective use of DS between May and October 2019. Fifteen patients had undergone CE and CT to assess for AL on postoperative day (POD) 7, and CT was performed just after CE. Univariate analysis was performed to assess the relationship between preoperative variables and the incidence of occult AL on POD 7

Results

The incidence of occult AL on postoperative day 7 was 6 of 15 (40%). Hand-sewn anastomosis, compared with stapled anastomosis, was a significant risk factor. Five more cases with occult AL that could not be detected with CE could be detected on CT following CE; CE alone had a 33% false-negative radiological result rate.

Conclusions

Hand-sewn anastomosis appeared to be a risk factor for occult AL, and CE alone had a high false-negative radiological result rate. When considering the introduction of early stoma closure, stapled anastomosis and CT following CE could be an appropriate inclusion criterion and preoperative examination, respectively.
Increased circulating total bile acid levels were associated with organ failure in patients with acute pancreatitis
Authors: Li Weiqin , Xie Xiaochun , Dong Jie , Lu Guotao , Gao Kun , Li Xiaoyao , Mao Wenjian , Chen Faxi , Tong Zhihui , Li Baiqiang 
Published: February 2020

Abstract
Background Recent studies have shown that bile acids (BAs) are closely related to metabolic and inflammatory diseases. Our study aimed to investigate whether circulating total bile acid (TBA) levels were associated with the severity of acute pancreatitis (AP). Methods We retrospectively collected data on patients diagnosed with AP in a tertiary center from 01 January 2014 to 31 December 2016. The highest TBA value during the first 1,2,3,5,7 days after admission was determined as D1, D2, D3, D5, D7 TBA max . Patients were divided into the high TBA (HTBA) group and the normal TBA (NTBA) group according to whether the TBA max was ≥10μmol/L. The prognosis and complications, including death, organ failure (OF) and pancreatic necrosis, were compared between the two groups. Logistic regression analysis and receiving operating characteristic (ROC) curve were used to evaluate the relationship between circulating TBA and organ failure in AP patients. Results Through stratified analysis of each time period, we found that the incidence of OF in the HTBA group was significantly higher than that in the NTBA group, and the AP severity classification in the HTBA group was more serious than that in the NTBA group. In addition, according to the D7 TBA max values, the pancreatic necrosis rate, percutaneous catheter drainage (PCD) rate and mortality in the HTBA group were higher than those in the NTBA group. Multivariate regression analysis showed that HTBA (odds ratio (OR), 4.894; P = 0.002) was an independent risk factor for AP complicated with OF, which was verified in the grouping based on D7 TBA max . ROC analysis revealed that a circulating D7 TBA max cutoff point of 6.450 umol/L had optimal predictive value for the development of OF in AP patients with an area under the curve of the ROC curve (AUCROC) of 0.777. Conclusions The increase of circulating TBA in early stage of AP is independently related to organ failure, which indicates the adverse prognosis of AP patients.
A practical staging atlas to study embryonic development of Octopus vulgaris under controlled laboratory conditions
Authors: Deryckere Astrid , Styfhals Ruth , Vidal Erica AG , Almansa Eduardo , Seuntjens Eve 
Published: February 2020

Abstract
Background Octopus vulgaris has been an iconic cephalopod species for neurobiology research as well as for cephalopod aquaculture. It is one of the most intelligent and well-studied invertebrates, possessing both long- and short-term memory and the striking ability to perform complex cognitive tasks. Nevertheless, how the common octopus developed these uncommon features remains enigmatic. O. vulgaris females spawn thousands of small eggs and remain with their clutch during their entire development, cleaning, venting and protecting the eggs. In fact, eggs incubated without females usually do not develop normally, mainly due to biological contamination (fungi, bacteria, etc.). This high level of parental care might have hampered laboratory research on the embryonic development of this intriguing cephalopod. Results Here, we present a completely parameter-controlled artificial seawater standalone egg incubation system that replaces maternal care and allows successful embryonic development of a small-egged octopus species until hatching in a laboratory environment. We also provide a practical and detailed staging atlas based on bright-field and light sheet fluorescence microscopy imaging for precise monitoring of embryonic development. The atlas has a comparative section to benchmark stages to the different scales published by Naef (1928), Arnold (1965) and Boletzky (2016). Finally, we provide methods to monitor health and wellbeing of embryos during organogenesis. Conclusion Besides introducing the study of O. vulgaris embryonic development to a wider community, this work can be a high-quality reference for comparative evolutionary developmental biology.
Review of health and non-health sector indicators for monitoring service provision along the continuum of care for maternal health
Authors: Mothupi Mamothena , Knight Lucia , Tabana Hanani 
Published: February 2020

Abstract
Objective : This study uses health and non-health sector data sources to select and assess available indicators for service provision along the continuum of care for maternal health at subnational levels in South Africa. It applies the adequacy approach established in another study to assess the multi-dimensionality of available indicators. Using adequacy and the process of assessment in the study, the comprehensiveness of the continuum of care for improving maternal health outcomes can be assessed.
Results: We found 27 indicators of care utilization and access, linkages of care, and quality of care from the routine district health information system. The General Household Survey contained 11 indicators for the social determinants of health on the continuum of care framework. Indicator gaps include health promotion during and after pregnancy, maternal nutrition, empowerment and quality of care. At present, the available indicators measure about 74% of the interventions on the continuum of care framework. We make recommendations regarding improvements needed to better measure and monitor the continuum of care for maternal health. These involve actions within the health system and include integration of non-health system indicators.
EFFECT: A Randomized Phase II Study of Efficacy and Impact on Function of Two Doses of Nab-paclitaxel as First-line Treatment in Older Women with Advanced Breast Cancer
Authors: Biganzoli Laura , Cinieri Saverio , Berardi Rossana , Pedersini Rebecca , McCartney Amelia , Minisini Alessandro Marco , Caremoli Elena Rota , Spazzapan Simon , Magnolfi Emanuela , Brunello Antonella , Risi Emanuela , Palumbo Raffaella , Leo Silvana , Colleoni Marco , Donati Sara , De Placido Sabino , Orlando Laura , Pistelli Mirco , Parolin Veronica , Mislang Anna , Becheri Dimitri , Puglisi Fabio , Sanna Giuseppina , Zafarana Elena , Boni Luca , Mottino Giuseppe 
Published: February 2020

Abstract
BackgroundLimited data are available on nab-paclitaxel in older breast cancer patients, with this population being under-represented in clinical trial. A weekly schedule is recommended, but there is a paucity of evidence regarding the optimal dose. We evaluated the efficacy of two different doses of weekly nab-paclitaxel, with a specific focus on their corresponding impact on patient function, in order to address the lack of data specifically relating to the older population.MethodsEFFECT is an open-label, phase II trial wherein 160 women with advanced breast cancer aged ≥ 65 years were enrolled from 15 institutions within Italy. Patients were randomly assigned 1:1 to receive nab-paclitaxel 100 mg/m2 (Arm A) or 125 mg/m2 (Arm B) on days 1,8,15 on a 28-day cycle, as first-line treatment for advanced disease. The primary endpoint was event-free survival (EFS), wherein an event was defined as disease progression (PD), functional decline (FD) or death.ResultsAfter a median follow-up of 32.6 months, 140 events were observed in 158 evaluable patients. Median EFS was 8.2 months (90% CI, 5.9-8.9) in Arm A, versus 8.3 months (90% CI, 6.2-9.7) in Arm B. Progression-free survival, overall survival and response rates were similar in both groups. A higher percentage of dose reductions and discontinuations due to adverse events (AEs) was noted in Arm B. The most frequently reported non-hematological AEs were fatigue (grade [G] 2-3 toxicity occurrence in Arm A versus B: 43% and 51%, respectively) and peripheral neuropathy (G2-3 Arm A versus B: 19% and 38%, respectively).ConclusionThe two weekly nab-paclitaxel regimens were comparable in all pre-specified outcomes. However, 100 mg/m2 was significantly better tolerated with fewer neurotoxicity-related events, representing a more feasible dose to be recommended for older patients with advanced disease.
Pretreatment Thrombocytosis as an Independent Predictive Factor for Chemoresistance and Poor Survival in Epithelial Ovarian Cancer
Authors: Nakao Sari , Minaguchi Takeo , Itagaki Hiroya , Hosokawa Yoshihiko , Shikama Ayumi , Tasaka Nobutaka , Akiyama Azusa , Ochi Hiroyuki , Matsumoto Koji , Satoh Toyomi 
Published: February 2020

Abstract
Background Thrombocytosis is related to tumor stage and survival of ovarian cancer in addition to common complications with malignant diseases, such as anemia and inflammation. The aim of our study was to clarify the precise prognostic impact of pretreatment thrombocytosis in epithelial ovarian cancer. Methods We retrospectively analyzed 280 consecutive patients who were treated for epithelial ovarian cancer at our institution between 2001 and 2011. Results Pretreatment thrombocytosis was observed in 18.9% of all the patients, and was associated with advanced FIGO stage, primary treatment, operation achievement, histologic subtype, microcytic hypochromic anemia (MHA), and non-malignant inflammatory condition (p=0.0018, 0.0028, 0.00050, 0.034, 0.00090 and 0.0022). In the patients who relapsed after primary adjuvant chemotherapy (n=126), thrombocytosis was associated with shorter treatment-free interval (TFI) (p=0.0091). The univariate and multivariate analyses revealed that thrombocytosis was independently associated with TFI and MHA (p=0.021 and 0.0091). Patients with thrombocytosis had worse progression-free survival (PFS) and overall survival (OS) than those without thrombocytosis (p<0.0001 and <0.0001). The multivariate analyses for prognostic factors demonstrated that thrombocytosis was significant for poor PFS and OS (p=0.0050 and p=0.022) independently of stage, histology, primary treatment, operation achievement, non-malignant inflammatory condition and MHA. Conclusions The current findings indicate that the detrimental survival impact of pretreatment thrombocytosis in epithelial ovarian cancer may be independent of tumor extent but rather attributed to chemoresistance, further supporting the therapeutic potential of targeting thrombopoietic cytokines in the disease.
Application of functional data analysis to classify communities according to the pattern of malaria incidence, to guide targeted control strategies.
Authors: DIENG Sokhna , Michel Pierre , Guindo Abdoulaye , Sallah Kankoe , Ba El-hadj , Cissé Badara , Carrieri Patrizia , Sokhna Cheikh , Milligan Paul , Gaudart Jean 
Published: February 2020

Abstract
Background Effective targeting of malaria control in low transmission areas requires identification of transmission foci or hotspots. We investigated the use of functional data analysis to identify and describe spatio-temporal pattern of malaria incidence in an area with seasonal transmission in west-central Senegal. Method Malaria surveillance was maintained over 5 years from 2008 to 2012 at health facilities serving a population of 500,000 in 575 villages in two health districts in Senegal. Smooth functions were fitted from the time series of malaria incidence for each village, using cubic B-spline basis functions. The resulting smooth functions for each village were classified using hierarchical clustering (Ward’s method), using several different dissimilarity measures. The optimal number of clusters was then determined based on four cluster validity indices, to determine the main types of distinct temporal pattern of malaria incidence. Epidemiological indicators characterizing the resulting malaria incidence pattern in terms of the timing of seasonal outbreaks, were calculated based on the slope (velocity) and rate of change of the slope (acceleration) of the incidence over time. Results Three distinct patterns of malaria incidence were identified. A pattern characterized by high incidence, in 12/575 (2%) villages, with average incidence of 114 cases/1000 person-years over the 5 year study period; a pattern with intermediate incidence in 97 villages (17%), with average incidence of 13 cases/1000 person-years; and a pattern with low incidence in 466 (81%) villages, with average incidence 2.6 cases/1000 person-years. Epidemiological indicators characterizing the fluctuations in malaria incidence showed that seasonal outbreaks started later, and ended earlier, in the low incidence pattern. Conclusion Functional data analysis can be used to classify communities based on time series of malaria incidence, and to identify high incidence communities. Indicators can be derived from the fitted functions which characterize the timing of outbreaks. These tools may help to better target control measures.
A Network Pharmacology-Based Study on Alzheimer disease prevention and treatment of Qiong Yu Gao
Authors: You Jieshu , Li Chen-yue , Chen Wei , Wu Xia-lin , Huang Li-jie , Li Ren-kai , Gao Fei , zhang Ming-yue , Liu Huan-lan , Qu Wei-ling 
Published: February 2020

Abstract
Background and objective: As the pathological mechanisms of AD is complex, increasing evidence have demonstrated Chinese Medicine with multi-ingredients and multi-targets may be more suitable for the treatment of diseases with complex pathogenesis. Therefore, the study was to preliminarily decipher the bioactive compounds and potential mechanisms of Qiong Yu Gao (QYG) for AD prevention and treatment by an integrated network pharmacology approach. Methods: Putative ingredients of QYG and significant genes of AD were retrieved from public database after screening. Then QYG ingredients target proteins/genes were obtained by target fishing. Compound-target-disease network was constructed using Cytoscape to decipher the mechanism of QYG for AD. KEGG pathway and GO enrichment analysis were performed to investigate the molecular mechanisms and pathways related to QYG for AD treatments. Results: Finally, 70 compounds and 511 relative drug targets were collected. In which, 17 representative direct targets were found. Gene ontology enrichment analysis revealed that the adenylate cyclase-inhibiting G-protein coupled acetylcholine receptor signaling pathway was the key biological processes and were regulated simultaneously by the 17 direct targets. KEGG pathway enrichment analysis found that three signaling pathways were closely related with AD treatment by QYG, including PI3K-Akt signaling pathway, regulation of actin cytoskeleton pathway and insulin resistance pathway. Conclusion: This study demonstrated that QYG exerted the effect of treating AD by regulating multi-targets with multi-components. Furthermore, the study demonstrated that a network pharmacology-based approach was useful for elucidation of the interrelationship between complex diseases and interventions of Chinese herbal medicines.
Increased risk of hyperlipidemia in patients with anxiety disorders: A population-based study
Authors: Chien I-Chia , Lin Ching-Heng 
Published: February 2020

Abstract
Objective This study examined the prevalence and incidence of hyperlipidemia among patients with anxiety disorders in Taiwan. Methods We used a large dataset containing random samples, and more than 766,000 subjects who were aged 18 years or older in 2005 were identified. Subjects who had more than one primary or secondary diagnosis of anxiety disorders were identified. Individuals who had a primary or secondary diagnosis of hyperlipidemia or medication treatment for hyperlipidemia were also identified. The prevalence rate of hyperlipidemia in patients with anxiety disorders with that of the general population in 2005 was compared. We then followed this cohort to monitor incident cases of hyperlipidemia in anxiety patients, and assessed whether a difference existed from the general population during the period 2006–2010. Results A higher prevalence rate of hyperlipidemia in patients with anxiety disorders was observed as compared with the general population (21.3% vs. 7.6%, odds ratio 2.14; 95% confidence interval, 2.07–2.22) in 2005. Additionally, a higher average annual incidence rate of hyperlipidemia in patients with anxiety disorders was also found as compared with the general population (5.49% vs. 2.50%, risk ratio 1.64; 95% confidence interval, 1.58–1.70) from 2006 to 2010. Conclusions Patients with anxiety disorders had higher prevalence and incidence rates of hyperlipidemia than the general population. Risk factors that were found to be associated with the higher incidence rate of hyperlipidemia among anxiety patients included a greater age, the female gender, and the presence of diabetes and hypertension.
Identification and validation of AKR1C1/2/3 as hepatocellular carcinoma risk modules via an integrated strategy
Authors: Xu Mingxing , Zhong Yuesi , Yang Fangji , Liu Kai , Zhuang Baoding , Xu Ruiyun , Ling Yunbiao , Lin Nan 
Published: February 2020

Abstract
Background The human aldo-keto reductase 1 (AKR1) C family comprises four enzymes, AKR1C1–AKR1C4. Lots of studies have investigated the function of AKR1Cs in tumors, however little is known in hepatocellular carcinoma (HCC). Methods Public databases were used to explore expression and role of AKR1Cs in HCC. Meanwhile, data of 134 HCC patients from Firebrowse website was used for validation. Results The results revealed that AKR1Cs expression was negatively correlated with the infiltration level of CD4+ T cells. Overexpression of AKR1C1/2/3 was significantly associated with tumor stage and pathological grade. Moreover, higher mRNA expression of AKR1C1/2/3 was related with shorter overall survival (OS), progression-free survival (PFS) and relapse-free survival (RFS). Multivariate Cox regression analysis showed that AKR1C1/2/3 could be significant risk factors for HCC patients. Additionally, genetic alterations of AKR1Cs can significantly affect patient OS and PFS, and expression of AKR1Cs was linked to functional networks involving oxidation-reduction process, cellular hormone metabolic process and organic hydroxy compound metabolic process, as well as retinol metabolism, steroid hormone biosynthesis, metabolic pathway and fatty acid degradation pathways. Conclusions In conclusion, we successfully elaborated the relationship between AKR1Cs expression and immune infiltrations, and identified AKR1C1/2/3 could be novel prognostic biomarkers for HCC patients.
Perspective of Healthcare Providers using Reproductive Smart Mobile Application in Jakarta: A Qualitative Study
Authors: Wiweko Budi , Riyanti Aida , Olivia Shanty , Priangga Muhammad , Silvana Vita , Zakirah Sarah Chairani , Pertiwi Ilonna Putri , Putro Anggi Lewis Reso , Wibawa Yohanes Satrya , Harzif Achmad Kemal , Pratama Gita , Sumapraja Kanadi , Muharam R. , Hestiantoro Andon 
Published: February 2020

Abstract
Objective : Our study aimed to evaluate the perspectives of health care providers of JAKPROS ( Jakarta Reproduksi Sehat ) application that is used to improve the maternal health service. This descriptive qualitative study engaged 16 healthcare providers from two sub-district hospitals. The participants were selected using purposive sampling and cross-sectional design. Participant were asked to fill the written informed consent.Data were collected by in-depth discussion of a list of selected questions in a focus group discussion. Results: Most health care providers used paper-based systems in their hospitals.They said that it was very helpful in helping them communicate with their patients and provide information. The application has many benefits that help doctors provide the best service for patients during pregnancy.The use and adoption of app is needed to make a further improvements in the healthcare system. This study will help improve the application for more widespread use in a greater number of hospitals. JAKPROS made healthcare provider easier to communicate and track patient’s health. This app also assist patients related to the doctor’s schedule, health information, and nearest hospital.
PDK2: a novel diagnostic and prognostic biomarker for liver cancer
Authors: Liu Zhi-Cheng , Li Yan-Qing , Jiao Yan , Zhao Yue-Chen 
Published: February 2020

Abstract
Background: Liver cancer (LC) is a common malignancy with very high morbidity. Pyruvate dehydrogenase kinases (PDKs) are regulators of mitochondrial pyruvate dehydrogenase complexes (PDCs) and play an important role in regulating cellular energy metabolism. In this study, The Cancer Genome Atlas (TCGA) database was used to analyze the expression of PDK2 mRNA in LC, and to explore the value of PDK2 in the diagnosis and prognosis of LC.Methods: The TCGA database, containing the clinical data of 373 LC patients, includes information on PDK2 expression values. The receiver operating characteristic (ROC) curve of PDK2 was drawn to evaluate its diagnostic ability. Patients were divided into PDK2 high- and low-expressing groups by threshold levels. The Chi-square test was used to evaluate the correlation between PDK2 levels and clinicopathological characteristics. The Kaplan-Meier estimator and Cox regression analysis were performed to assess the effect of PDK2 levels on survival outcomes.Results: PDK2 expression in LC tissue was lower than that in normal liver tissues. According to the area under the curve (AUC) value calculated by ROC, PDK2 has a considerable diagnostic value for LC prognosis. The decreased expression of PDK2 is associated with clinical parameters, such as histologic grade ( P =0.0001), radiation therapy ( P =0.0490), vital status ( P =0.0240), and overall survival (OS) ( P =0.0222). Multivariate analysis shows that decreased PDK2 level is an independent risk factor for predicting poor prognosis in LC.Conclusions: PDK2 has a significant impact on the prognosis of LC and is a potential biomarker for the diagnosis and prognosis of LC.