bluuebunny/crossref_metadata_2025_split huggingface dataset:
PSXIV-15 Metagenomic sequencing of rumen microorganisms of cattle fed a corn stover-based diet
Authors: Piña-Gónzalez Laura , Miranda-Ríos Juan , Alonso-Morales Rogelio Alejandro , Maya Otoniel , Corona Luis , Márquez-Mota Claudia Cecilia 
Published: December 2019

Abstract
Worldwide, there is a need to discover new microorganisms that efficiently degrade lignocellulosic complexes that would help to improve the digestibility of low-quality agricultural byproducts. The aim of the study was to evaluate the effects of a corn stover-based diet (CSD) on rumen bacteria. Ruminal fluid of 6 Holstein cows (595 ± 96 kg) was collected during two periods. During first period, animals were consuming a diet based on corn silage and oat hay (DB), mineral premix and water ad libitum (50:50, DM). In second period, animals were provided a CSD (100% DM), mineral premix and water ad libitum for 45 days. Ruminal fluid was collected through esophageal tube, filtered and stored at -80°C until DNA extraction. Rumen microorganisms were identified by sequencing the 16SrRNA gene using the Illumina Miseq platform and primers for V3 and V4 regions. Data were analyzed by QIIME 1.9. Analysis of variance was performed for a completely randomized design using the MIXED procedure of SAS 9.1. The taxonomic affiliation showed that both populations were mainly composed of Firmicutes, Bacteroidetes and Proteobacteria. The most abundant bacteria species in both diets were Ruminococcus flavefaciens, Prevotella copri, Prevotella ruminicola, Fibrobacter succinogenes, Bacillus coagulans, Bacteroides uniformis and Selenomonas ruminantium. Feeding a CSD, increased the relative abundance of Prevotella ruminicola (from 6.1 to 20.9%, P &lt; 0.01), Streptococcus luteciae (from 0.05 to 0.78%, P &lt; 0.01), Clostridium aminophilum (0.45 to 3.1%, P &lt; 0.01), Selenomonas ruminantium (5.2 to 21.8%, P &lt; 0.02) and Pantoea agglomerans (0.7 to 3.9%, P &lt; 0.01) and decreased Propionibacterium acnes (0.7 to 0.1%, P &lt; 0.02) and Bacteroides ovatus (0.9 to 0.1%, P &lt; 0.01). Feeding cattle with a diet with a more lignified forage like CSD led to the proliferation of bacteria such as Prevotella ruminicula, Streptococcus luteciae, Clostridium aminophilum, Selenomonas ruminantium and Pantoea agglomerans.
PSX-26 Levels of forage and narasin inclusion on rumen fermentation in feedlot lambs
Authors: Sardinha Lairana A , Polizel Daniel M , Miszura Alexandre A , Limede Arnaldo C , Barroso José P R , Martins André S , Oliveira Gabriela B , Ferreira Evandro M , Eckermann Nathalia R , Vaz Pires Alexandre 
Published: December 2019

Abstract
The objective of this study was to determine the effects of forage levels and narasin inclusion in diets on the rumen parameters in feedlot lambs. Forty-four lambs were allotted in a randomized complete block design, defined by initial body weight, in a 2x2 factorial arrangement. The first factor was forage inclusion (10 or 20% of coastcross hay, DM basis) and the second factor was narasin (0 or 13 ppm). The experimental diets were isonitrogenous (17.4%, DM basis). At the end of the experimental period, the lambs were slaughtered without fasting, and the rumen fluid was collected to determinate pH and short chain fatty acid (SCFA). Data were analyzed using the MIXED procedure of SAS and the LSMEANS option was used to obtain the means. The effects were considered significant when P &lt; 0.05. There was an interaction between hay and narasin on the molar proportion of propionate (P = 0.02) and the acetate:propionate ratio (P &lt; 0.01). Narasin inclusion in diets containing 20% forage increased propionate (25.4 vs 37.1 mM/100mM; P = 0.02); however, there was no effect in diets containing 10% forage (35.9 ± 1.75 mM/100Mm). Consequently, the narasin decreased acetate:propionate ratio in diets containing 20% forage (2.57 vs 1.42; P &lt; 0.01), and no effect was observed 10% forage diets (1.40 ± 0.14). The inclusion of narasin increased the total SCFA (86.2 vs 115.4 mM; P &lt; 0.01), ruminal pH (6.26 vs 6.49; P = 0.05) and decreased acetate (55.6 vs 47.6 mM/100mM; P &lt; 0.01). The inclusion of 20% forage increased acetate when compared with 10% hay (54.99 vs 48.19 mM/100mM; P &lt; 0.01) and the pH ruminal (6.25 vs 6.49; P = 0.04). The experimental diets did not affect the butyrate. In summary, the narasin inclusion and forage levels content change rumen parameters in feedlot lambs.
Cyberbullying Perpetration and Victimization in Youth: A Meta-Analysis of Longitudinal Studies
Authors: Marciano Laura , Schulz Peter J , Camerini Anne-Linda 
Published: December 2019

AbstractCyberbullying perpetration (CP) and victimization (CV) are prevalent issues in adolescent development. However, previous meta-analyses focused only on cross-sectional findings. The present study aims to meta-analytically summarize 56 longitudinal studies on CP, CV, and related factors in children and adolescents. Forty meta-analyses on CP and CV as both predictors and outcomes of person-related, Internet activity-related, and contextual factors were performed. Additional moderator analyses took into account study design, sample population, and quality of the studies. Results highlight reciprocal longitudinal effects among CP, CV, and both traditional bullying and victimization: CP causes externalizing problem behaviors over time, whereas CV constitutes a risk factor of internalizing problems, such as depression and anxiety. Conversely, behavioral problems and increased Internet use predict involvement in CP, while depression, anxiety, and Internet use also predict CV over time. Further longitudinal research is needed to provide empirical evidence on understudied concepts.
The Purposes of Starvation
Authors: Conley Bridget , de Waal Alex 
Published: December 2019

Abstract
Mass starvation has throughout history been mis-categorized as a natural phenomenon, or an unfortunate side-effect of conflict and political oppression. The numbers and names of the victims fade into the background, blurred traces of the horrors of history. Yet, this is both an inaccurate understanding of starvation crimes and an injustice to victims. Mass starvation is a process of deprivation that occurs when actors impede the capacity of targeted persons to access the means of sustaining life. In this article, we introduce key features of starvation and note that many of the acts that create conditions of mass starvation are already prohibited under different provisions of international law. We introduce the term ‘starvation crimes’ to capture how these separately criminalized acts, when perpetrated over a long duration can create mass starvation. Implicit in ‘starvation crimes’ is that starvation is produced by leaders’ decisions and serves political, military or economic goals. We discuss nine objectives that can be furthered through mass starvation, offering historical examples to illustrate each. They include: (i) extermination or genocide; (ii) control through weakening a population; (iii) gaining territorial control; (iv) flushing out a population; (v) punishment; (vi) material extraction or theft; (vii) extreme exploitation; (viii) war provisioning; and (ix) comprehensive societal transformation.
Pollen Resources Used by Two Species of Stingless Bees (Meliponini) in a Tropical Dry Forest of Southern Ecuador
Authors: Gaona Fernando P , Guerrero Anthony , Gusmán Elizabeth , Espinosa Carlos Iván 
Published: December 2019

Abstract
Pollinators are crucial for ecosystem functionality; however, little is known about the plant species used by some of these, such as stingless bees. In this study, for the first time, pollen resources used by Melipona mimetica Cockerell (Hymenoptera: Apidae: Meliponini) and Scaptotrigona sp. Moure (Hymenoptera: Apidae: Meliponini) were identified through analysis of corbicular pollen found on worker bees in a dry forest in southern Ecuador. In total, 68 pollen types were identified belonging to 31 botanical families. The most represented plant families were Fabaceae (16%), Malvaceae (7%), and Boraginaceae (7%). Both stingless bee species exhibited a polylectic behavior, with an average of 16 pollen types collected by individual bees. Differences in abundances of pollen types collected by each species indicated distinct uses for these two bee species.
Detection of Amblyomma mixtum (Acari: Ixodidae) in Germany on a Human Traveler Returning From Cuba
Authors: Chitimia-Dobler Lidia , Schaper Sabine , Mansfeld Peter , Gonschorrek Joachim , Bröker Michael , Nava Santiago 
Published: December 2019

Abstract
The importation of exotic ticks to Germany on infested humans is rarely reported. Here we describe the detection of an Amblyomma mixtum nymph harboring Rickettsia amblyommatis by a tourist returning from a holiday trip to Cuba. Tick infestation may be neglected by tourists. Therefore, careful anamnesis and diagnosis should be carried out when tourists return from tropical and subtropical countries suffering from nonspecific symptoms, such as fever and malaise.
Successful management of severe coronoid process hyperplasia in a patient with mucopolysaccharidosis VI: a case report
Authors: Khadembaschi Darius , Arvier John , Beech Nicholas , Dolan David , McGill Jim 
Published: December 2019

Abstract
Mucopolysaccharidoses are a group of rare lysosomal storage diseases caused by a deficiency of enzymes, which breakdown glycosaminoglycans, with consequent dysfunction of affected tissues. Mandibular coronoid hyperplasia, with associated trismus, has been recently described as a feature of the craniofacial abnormalities seen in these patients. However, the details of the surgical and post-operative management of these patients have not been previously documented. This case describes the successful management of severe trismus from coronoid process hyperplasia in a 14-year-old male, utilising an extra-oral approach for bilateral coronoidectomies and removal of exophytic zygomatic bone, followed by immediate and long-term physiotherapy. An improvement of mandibular opening from 8 to 45 mm has been maintained at 18 months post operation.
The influence of metagalactic ultraviolet background fluctuations on the high-redshift Lyα forest
Authors: Meiksin Avery 
Published: December 2019

ABSTRACT
Under the assumption that galaxies and quasi-stellar objects (QSOs) dominate the metagalactic ultraviolet (UV) background, it is shown that at high redshifts, fluctuations in the UV background are dominated by QSO shot noise and have an autocorrelation length of a few to several comoving Mpcs, depending on the bright end of the QSO luminosity function. The correlations create long-range spatial coherence in the neutral hydrogen fraction. Using a semi-analytic model, it is demonstrated that the coherence may account for the broad distribution in effective optical depths measured in the Lyα forest spectra of background QSOs, for line-of-sight segments of comoving length $50\, h^{-1}$ Mpc at redshifts 5 &lt; z &lt; 6. Capturing the fluctuations in a numerical simulation requires a comoving box size of ∼1 Gpc, although a box half this size may be adequate if sufficient random realizations of the QSO population are performed.
The use of particle distributions in Solar system small body dynamics
Authors: Kastinen Daniel 
Published: December 2019

ABSTRACTThe extraterrestrial material, called meteoroids, that constantly enters the Earth’s atmosphere gives us a unique opportunity to examine the motion and population of small bodies in the Solar system. This exploration requires simulating the motion of these particles. Currently, only the timing of meteoroids encountering the Earth is well predicted by such simulations, while other parameters are uncertain. This can be remedied by proper stochastic representation and estimation using a sufficient number of samples. We propose methods to both represent simulations in a stochastic manner and to improve sampling using Importance Sampling. We also demonstrate these methods practically with a test model. Using the test model resulted in an error reduction by a factor of 3 without increase in computation time. Thus, we validated that these techniques can be implemented on and are compatible with Solar system small body dynamics models. Based on these results we predict that when properly implemented on a larger and more complex model, Importance Sampling can improve sampling numbers by several orders of magnitude without increasing computation time, depending on the simulation in question. The methods presented here bring advantages such as; greatly reduced estimation errors, fitting models without re-running simulations, model comparisons without sample variations, circumventing unknown properties using invariant measures, representing large particle numbers without additional errors. This methodology has wide application possibility and will enable larger, more reliable and reusable simulations of dynamical astronomy.
Momentum injection by clustered supernovae: testing subgrid feedback prescriptions
Authors: Gentry Eric S , Madau Piero , Krumholz Mark R 
Published: December 2019

ABSTRACT
Using a 1D Lagrangian code specifically designed to assess the impact of multiple, time-resolved supernovae (SNe) from a single-star cluster on the surrounding medium, we test three commonly used feedback recipes: delayed cooling (e.g. used in the gasoline-2 code), momentum-energy injection (a resolution-dependent transition between momentum-dominated feedback and energy-dominated feedback used, e.g. in the fire-2 code), and simultaneous energy injection (e.g. used in the EAGLE simulations). Our work provides an intermediary test for these recipes: we analyse a setting that is more complex than the simplified scenarios for which many were designed, but one more controlled than a full galactic simulation. In particular, we test how well these models reproduce the enhanced momentum efficiency seen for an 11 SN cluster simulated at high resolution (0.6 pc; a factor of 12 enhancement relative to the isolated SN case) when these subgrid recipes are implemented in low resolution (20 pc) runs. We find that: (1) the delayed cooling model performs well – resulting in 9 times the momentum efficiency of the fiducial isolated SN value – when SNe are clustered and 1051 erg are injected per SN, while clearly overpredicting the momentum efficiency in the single SN test case; (2) the momentum-energy model always achieves good results, with a factor of 5 boost in momentum efficiency; and (3) injecting the energy from all SNe simultaneously does little to prevent overcooling and greatly underproduces the momentum deposited by clustered SNe, resulting in a factor of 3 decrease in momentum efficiency on the average.
Spectral library of age-benchmark low-mass stars and brown dwarfs
Authors: Manjavacas E , Lodieu N , Béjar V J S , Zapatero-Osorio M R , Boudreault S , Bonnefoy M 
Published: December 2019

ABSTRACT
In recent years, some extremely red brown dwarfs have been discovered. They were believed to have a low surface gravity, but many of their spectral characteristics are similar to those of high-surface-gravity brown dwarfs, showing that the spectral characteristics of young brown dwarfs are poorly understood. We aim to test surface-gravity indicators in late-M and early-L brown dwarf spectra using data obtained with the X-shooter spectrograph at the Very Large Telescope. We select a benchmark sample of brown dwarf members of Chamaeleon I (∼2 Myr), Upper Scorpius (5−10 Myr), the Pleiades (132 ± 27 Myr) and Praesepe (590−790 Myr) with well-constrained ages and similar metallicities. We provide a consistent spectral classification of the sample in the optical and in the near-infrared. We measure the equivalent widths of their alkali lines, finding that they have a moderate correlation with age, especially for objects with spectral types M8 and later. We use spectral indices defined in the literature to estimate surface gravity, finding that their gravity assignment is accurate for 75 per cent of our sample. We investigate the correlation between red colour and age, finding that after ∼10 Myr, the colour does not change significantly for our sample with spectral types M6.0–L3.0. In this case, the red colours might be associated with circumstellar discs, ring structures, extinction, or viewing angle. Finally, we calculate the bolometric luminosity, and J and K bolometric corrections for our sample. We find that six objects are overluminous compared with other members of the same association. These objects are flagged as binary candidates by the Gaia survey.
De novo diploid genome assembly for genome-wide structural variant detection
Authors: Zhang Lu , Zhou Xin , Weng Ziming , Sidow Arend 
Published: December 2019

AbstractDetection of structural variants (SVs) on the basis of read alignment to a reference genome remains a difficult problem. De novo assembly, traditionally used to generate reference genomes, offers an alternative for SV detection. However, it has not been applied broadly to human genomes because of fundamental limitations of short-fragment approaches and high cost of long-read technologies. We here show that 10× linked-read sequencing supports accurate SV detection. We examined variants in six de novo 10× assemblies with diverse experimental parameters from two commonly used human cell lines: NA12878 and NA24385. The assemblies are effective for detecting mid-size SVs, which were discovered by simple pairwise alignment of the assemblies’ contigs to the reference (hg38). Our study also shows that the base-pair level SV breakpoint accuracy is high, with a majority of SVs having precisely correct sizes and breakpoints. Setting the ancestral state of SV loci by comparing to ape orthologs allows inference of the actual molecular mechanism (insertion or deletion) causing the mutation. In about half of cases, the mechanism is the opposite of the reference-based call. We uncover 214 SVs that may have been maintained as polymorphisms in the human lineage since before our divergence from chimp. Overall, we show that de novo assembly of 10× linked-read data can achieve cost-effective SV detection for personal genomes.
Examining Procedural Unfairness and Credibility Findings in the UK Asylum System
Authors: Campbell John R 
Published: December 2019

Abstract
This article addresses a key problem confronted by immigration judges (IJs) in their assessment of the asylum claims of unaccompanied asylum-seeking children – who are often not allowed to speak or participate in their own hearings – namely the manner in which asylum legal procedure is intertwined with an IJ’s decision to refuse the claim on the basis of adverse credibility. This article has three linked aims: to examine research that looks at how IJs decide credibility; to set out an ethnographic approach to better understand IJs’ decision-making; and to argue that asylum tribunals need to adopt appropriate guidelines.
True nutrient and amino acid digestibility of dog foods made with human-grade ingredients using the precision-fed cecectomized rooster assay1
Authors: Oba Patrícia M , Utterback Pamela L , Parsons Carl M , Swanson Kelly S 
Published: December 2019

AbstractFor a pet diet to be labeled as human-grade, every ingredient and the finished food must be stored, handled, processed, and transported according to the current good manufacturing practices for human edible foods. Human-grade dog foods are now available and increasing in popularity, but little research has been conducted to test the digestibility of these foods. For this reason, the objective of this experiment was to determine the true nutrient and amino acid (AA) digestibilities of dog foods formulated with human-grade ingredients using the precision-fed cecectomized rooster assay. Six commercial dog foods were tested, including the Beef & Russet Potato (BRP), Chicken & White Rice (CWR), Fish & Sweet Potato (FSP), Lamb & Brown Rice (LBR), Turkey & Whole Wheat Macaroni (TWM), and Venison & Squash (VSR) formulas provided by Just Food For Dogs LLC (Irvine, CA). Before analysis, all foods were lyophilized and ground. A precision-fed rooster assay using cecectomized roosters was conducted to determine the true nutrient digestibility and standardized AA digestibilities of the foods tested. Conventional roosters were used to determine the nitrogen-corrected true metabolizable energy (TMEn) of the foods. All animal procedures were approved by the University of Illinois Institutional Animal Care and Use Committee prior to experimentation. The substrates and rooster excreta were analyzed for macronutrient and AA composition. All data were analyzed using the Mixed Models procedure of SAS (version 9.4; SAS Institute, Cary, NC). In general, all foods tested were highly digestible. Dry matter digestibility was similar among CWR, LBR, and TWR foods, and greater (P &lt; 0.0001) than that of FSP and VSR foods. Organic matter digestibility was highest (P = 0.0002) for CWR and lowest (P = 0.0002) for VSR. For the majority of indispensable AA, digestibilities were greater than 85%, with some being greater than 90%. TMEn was higher (P &lt; 0.0001) for BRP than the other foods, which were similar to one another. Also, TMEn values were much higher than what would be estimated by using modified Atwater factors and often above the predictive equations for metabolizable energy (ME) recommended by the National Research Council or by using Atwater factors. Although statistical differences were observed among foods, they all performed well and the foods tested had very high AA digestibilities. Additionally, the TMEn data suggest that existing methods and equations for ME prediction underestimate the energy content of the foods tested.
Protection of telomeres 1 (POT1) of Pinus tabuliformis bound the telomere ssDNA
Authors: Luo Mei , Teng Xiaotong , Wang Bing , Zhang Jiaxue , Liu Yadi , Liu Di , Li Hui , Lu Hai 
Published: December 2019

Abstract
Protection of telomeres 1 (POT1) is a telomeric protein that binds to the telomere single-stranded (ss) region. It plays an essential role in maintaining genomic stability in both plants and animals. In this study, we investigated the properties of POT1 in Pinus tabuliformis Carr. (PtPOT1) through electrophoretic mobility shift assay. PtPOT1 harbored affinity for telomeric ssDNA and could bind plant- and mammalian-type ssDNA sequences. Notably, there were two oligonucleotide/oligosaccharide binding (OB) folds, and OB1 or OB2 alone, or both together, could bind ssDNA, which is significantly different from human POT1. Based on our data, we hypothesized that the two OB folds of PtPOT1 bound the same ssDNA. This model not only provides new insight into the ssDNA binding of PtPOT1 but also sheds light on the functional divergence of POT1 proteins in gymnosperms and humans.