bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pelatihan dan Pendampingan Penyusunan Proposal Penelitian Tindakan Kelas (PTK) bagi Guru IPA di kabupaten Hulu Sungai Tengah
Authors: Zainuddin Zainuddin , Dewantara Dewi , Wati Mustika , Misbah Misbah , Suyidno Suyidno , Haryandi Surya , Rahmatullah Panji , Munir M Jiddan Mishbahul 
Published: May 2020

Kegiatan pengabdian masyarakat ini bertujuan untuk meningkatkan pemahaman dan kemampuan guru-guru IPA di Kabupaten Hulu Sungai Tengah tentang PTK. Kegiatan pelatihan ini bertempat di SMPN 10 Barabai yang dihadiri oleh 36 orang guru IPA di Kabupaten Hulu Sungai Tengah yang tergabung dalam Musyawarah Guru Mata Pelajaran (MGMP) IPA. Kegiatan dilaksanakan menggunakan metode ceramah, tanya jawab, diskusi dan pendampingan pada saat penyusunan proposal. Kesimpulan dari hasil kegiatan pengabdian masyarakat ini adalah pelatihan dan pendampingan penyusunan proposal PTK bagi guru IPA di kabupaten Hulu Sungai Tengah telah dilaksanakan dengan lancar dan mendapat respon yang positif dari para peserta. Pelatihan ini telah memberikan informasi bagi guru tentang penyusunan proposal PTK. Dengan demikian, para guru mampu memahami dan menyusun proposal penelitian tindakan kelas. Setelah pelatihan ini, guru diharapkan mampu membuat proposal penelitian tindakan kelas serta melaksanakan penelitian tindakan kelas untuk mengatasi berbagai permasalahan yang ada dikelasnya. This community service activity aims to improve the understanding and ability of natural science teachers in the Hulu Sungai Tengah Regency about Classroom Action Research (CAR). This training activity took place at SMPN 10 Barabai, which was attended by 36 science teachers in the Hulu Sungai Tengah Regency who were members of the Science Teachers' Conference (MGMP). Activities were carried out using lecture, question and answer, discussion, and assistance methods when preparing proposals. The conclusion from the results of this community service activity was the training and mentoring for the preparation of CAR proposals for science teachers in the Hulu Sungai Tengah district, which had been carried out smoothly and received positive responses from the participants. This training has provided information for teachers about the preparation of CAR proposals. Thus, the teachers are able to understand and prepare class action research proposals. After this training, teachers are expected to be able to make class action research proposals and carry out classroom action research to overcome various problems in their class.
Production Forecasting: Optimistic and Overconfident—Over and Over Again
Authors: Bratvold Reidar B. , Mohus Erlend , Petutschnig David , Bickel Eric 
Published: May 2020

Summary
The oil and gas industry uses production forecasts to make decisions, which can be as mundane as whether to change the choke setting on a well, or as significant as whether to develop a field. These forecasts yield cash flow predictions and value-and-decision metrics such as net present value and internal rate of return.
In this paper, probabilistic production forecasts made at the time of the development final investment decisions (FIDs) are compared with actual production after FIDs, to assess whether the forecasts are optimistic, overconfident, neither, or both.
Although biases in time-and-cost estimates in the exploration and production (E&P) industry are well documented, probabilistic production forecasts have yet to be the focus of a comprehensive, public study. The main obstacle is that production forecasts for E&P development projects are not publicly available, even though they have long been collected by the Norwegian Petroleum Directorate (NPD), a Norwegian government agency. The NPD's guidelines specify that at the time of FID, the operators should report the forecasted annual mean and P10/90 percentiles for the projected life of the field.
We arranged to access the NPD database in order to statistically compare annual production forecasts given at the time of FID for 56 fields in the 1995 to 2017 period, with actual annual production from the same fields. This work constitutes the first public study of the quality of probabilistic production forecasts. The main conclusions are that production forecasts that are being used at the FID for E&P development projects are both optimistic and overconfident, leading to poor decisions.1
1
The conclusions based on the analysis presented in this paper are limited to the set of fields from the NCS. However, other authors have demonstrated the optimism bias in production forecasts from fields around the world (Nandurdikar and Wallace 2011; Nandurdikar and Kirkham 2012).
Application of Miscible Ethane Foam for Gas EOR Conformance in Low-Permeability Heterogeneous Harsh Environments
Authors: Salman Mohamad , Kostarelos Konstantinos , Sharma Pushpesh , Lee Jae Ho 
Published: May 2020

SummaryUnconventional plays pose a challenging set of operational conditions, including high temperature, high salinity, low permeability, and fracture networks. Aggressive development of these plays and the low primary recovery factors present an opportunity for using enhanced oil recovery (EOR) methods. This work presents a laboratory investigation of miscible ethane (C2H6) foam for gas EOR conformance in low-permeability, heterogeneous, harsh environments [&lt;15 md, 136,000 ppm total dissolved solids (TDS) with divalent ions, 165°F]. The use of C2H6 as an alternative to carbon dioxide (CO2) offers several operational and availability strengths, which might expand gas EOR applications to depleted or shallower wells. Coupling gas conformance also helps improve displacement efficiencies and maximize overall recovery. Minimum miscibility pressure (MMP) displacement tests were performed for dead crude oil from the Wolfcamp Spraberry Trend area using C2H6 and CO2. Aqueous stability, salinity scan, and static foam tests were performed to identify a formulation. Subsequent foam quality and coreflood displacement tests in heterogeneous carbonate outcrop cores were conducted to compare the recovery efficiencies of three processes: gravity-unstable, miscible C2H6 foam; gravity-stable, miscible C2H6; and gravity-unstable, miscible C2H6 processes. Slimtube tests comparing C2H6 to CO2 resulted in a lower MMP value for C2H6. We identified a stable surfactant blend capable of Type I microemulsion and persistent foams in the presence of oil. Corefloods conducted with gravity-unstable miscible C2H6 foam, gravity-stable miscible C2H6, and gravity-unstable miscible C2H6 recovered 98.4, 61.9, and 42.6% oil originally in place, respectively. Our work shows that miscible C2H6 injection processes achieved significant recoveries even under gravity-unstable conditions. The addition of foam provides better conformance control, enhancing overall recovery at the laboratory scale, showing promise for field applications.
Modeling Fracture Closure with Proppant Settling and Embedment during Shut-In and Production
Authors: Zheng Shuang , Manchanda Ripudaman , Sharma Mukul M. 
Published: May 2020

Summary
Fracture closure and proppant settling are two fully coupled processes during both shut-in and production. Proppant distribution greatly affects the residual fracture width and conductivity evolution, whereas fracture closure might limit proppant settling and force the proppant to crush or embed into the rock. Modeling fracture closure with proppant settling and embedment is challenging because of the multiple coupled physical processes involved, large time-scale differences, and extreme nonlinearity in the coupling of the processes. Conventional fracture-closure models either use simplified analytical estimates of the stress-dependent permeability of the reservoir or explicitly calculate the fracture width using empirical relationships, without considering the effect of fluid leakoff and dynamic changes in the proppant distribution in the fracture. In this work, we use a novel fully implicitly coupled fracturing/reservoir simulator to study fracture closure and proppant-settling/embedment processes during shut-in and production. This simulator implicitly couples the reservoir (rock deformation and porous flow), fracture (fracturing-fluid flow, proppant transport), and wellbore (slurry distribution, production) domains. During shut-in, a modified Barton-Bandis (Bandis et al. 1983) formula is used to describe the nonlinear relationship between the contact force and the residual fracture aperture considering the dynamic proppant spatial distribution and rock heterogeneity. During production, fracture conductivity is evaluated according to proppant distribution and further fracture closure caused by proppant crushing and embedment. A Newton-Raphson method is applied to solve the coupled system of equations.
Results from the simulations clearly show that typical periods of shut-in after fracturing lead to the formation of proppant banks at the bottom of the fracture in low-permeability, low-leakoff formations. This can lead to near-wellbore tortuosity and poor connectivity between the wellbore and the hydraulic-fracture network. Stress-dependent permeability, likely induced by induced unpropped fractures, is shown to be essential to obtain reasonable values of leakoff and to history match production trends. Proppant embedment is shown to be an important factor controlling production-decline rates in clay-rich shales.
Propionic acid induces dendritic spine loss by MAPK/ERK signaling and dysregulation of autophagic flux
Authors: Choi Hyosun , Kim In Sik , Mun Ji Young 
Published: May 2020

Abstract
Propionic acid (PPA) is a short-chain fatty acid that is an important mediator of cellular metabolism. It is also a by-product of human gut enterobacteria and a common food preservative. A recent study found that rats administered with PPA showed autistic-like behaviors like restricted interest, impaired social behavior, and impaired reversal in a T-maze task. This study aimed to identify a link between PPA and autism phenotypes facilitated by signaling mechanisms in hippocampal neurons. Findings indicated autism-like pathogenesis associated with reduced dendritic spines in PPA-treated hippocampal neurons. To uncover the mechanisms underlying this loss, we evaluated autophagic flux, a functional readout of autophagy, using relevant biomedical markers. Results indicated that autophagic flux is impaired in PPA-treated hippocampal neurons. At a molecular level, the mitogen-activated protein kinase (MAPK)/extracellular signal-regulated kinase (ERK) pathway was activated and autophagic activity was impaired. We also observed that a MAPK inhibitor rescued dendritic spine loss in PPA-treated hippocampal neurons. Taken together, these results suggest a previously unknown link between PPA and autophagy in spine formation regulation in hippocampal neurons via MAPK/ERK signaling. Our results indicate that MAPK/ERK signaling participates in autism pathogenesis by autophagy disruption affecting dendritic spine density. This study may help to elucidate other mechanisms underlying autism and provide a potential strategy for treating ASD-associated pathology.
SETD1A augments sorafenib primary resistance via activating YAP in hepatocellular carcinoma
Authors: wu jugang , Li Feng , Xia Yan , Chai Hongjuan , Gu Yan 
Published: May 2020

Abstract
Background: Sorafenib, the approved first-line chemotherapy drug for HCC, remains the key treatment agent which can effectively improve the survival rate of advanced HCC patients. However, the sorafenib primary resistance limits the application of sorafenib for HCC treatment. The aims of current study are to explore the role and mechanism of SETD1A (Histone Lysine Methyltransferase SET Domain Containing 1A) in sorafenib primary resistance. Methods: The expression of SETD1A in HCC was analyzed by Gene Expression Profiling Interactive Analysis. The survival of HCC patients was analyzed by KM plotter: Kaplan-Meier Plotter. Western Blot and Real-time qPCR were performed to measure the protein and mRNA levels, respectively. Cell counting kit-8 assay and colony formation assay were performed to determine cell viability and proliferation. Propidium Iodide and Trypan Blue staining assays were performed to investigate cell death. Results: Here, we showed that the expression of SETD1A was markedly upregulated in both HCC cell lines and tumor tissues compared to normal hepatocytes and corresponding non-tumor liver tissues, respectively. The patients who had higher level of SETD1A underwent lower survival rate of overall and sorafenib treated HCC patients, respectively. In addition, SETD1A expression was positively correlated with the IC50 of sorafenib treated HCC cell lines. Furthermore, we indicated that knockdown of SETD1 augmented proliferation inhibition and cell death induced by sorafenib. SETD1A deficiency impaired YAP phosphorylation and activation. YAP activation contributed to SETD1A mediated sorafenib primary resistance. Conclusions: Taken together, the current study demonstrated that STED1A enhanced YAP activation to induce sorafenib primary resistance in HCC.
A techno-practical method for overcoming the biotoxicity and volatility obstacles of butanol and butyric acid during whole-cell catalysis by Gluconobacter oxydans
Authors: Hua Xia , Du GenLai , Zhou Xin , Ali Nawaz , Ikram Haq ul , Xu Yong 
Published: May 2020

Abstract
Background: Butyric acid is a platform chemical material, the production of which has been greatly stimulated by the diverse range of downstream applications in many industries. In particular, higher quality butyric acid used in food and medicine, is more dependent on microbiological production methods. Hence, the bio-oxidation of butanol to butyric acid has been identified as a promising method with good potential economic and environmental benefits. However, both butanol and butyric acid usually performs intensive toxicity on most microorganisms as well as the bio-oxidation pathway. To develop a green, efficient and competitive microbiological method is the primary work to overcome the bottleneck of butyric acid industry. Result: A combined bioprocess was designed with alternative whole-cell catalysis for butyric acid bio-conversion from butanol by Gluconobacter oxydans in a sealed-oxygen supply bioreactor (SOS). In the operation system, the escape of volatile substrates and toxic chemicals to cells can be avoided by the use of a sealed bioreactor, combined with the rejuvenation of cells by supplying energy co-factors. Finally, during one batch whole-cell catalysis, the utilization rate of substrate increased from 56.6% to 96.0% by the simply skill. Additionally, the techno-practical bioprocess can realize the purpose of cell-recycling technology through the rejuvenation effect of co-factor. Finally, we obtained 135.3 g/L butyric acid and 216.7 g/L sorbose during 60 h whole-cell catalysis. This techno-practical technology provides a promising approach to promote the industrial production of butyric acid with more competitiveness. Conclusion: The techno-practical biotechnology has powerfully promoted the process of butyric acid production by microorganism, especially makes up for the lack of aerobic fermentation in the industry, and surmount the shortcomings of traditional anerobic fermentation. At the same time, this technically-practical system provides a promising approach for the promotion of the industrial production of butyric acid in a more competitive manner.
Disability-adjusted Life Years for respiratory syncytial virus in Colombian children.
Authors: buendia jefferson , Polack Fernando , Villamil Juana Patricia Sanchez 
Published: May 2020

Abstract
BACKGROUND Respiratory syncytial virus infection is the leading cause of bronchiolitis worldwide. However, little is known about the real impact of bronchiolitis on society in terms of years of life lost due to this condition. The objective of the present study is to determine the Disability-Adjusted Life Years (1) in ColombiaMETHODS Data from the national epidemiological surveillance system were used to estimate DALYs, calculated from the sum of years of life lost and years lived with disability due to RSV infection in Colombia. A bootstrapped method with 10000 iterations was used to estimate each statistical parameter using the package DALY calculator in R.RESULTS In 2019, 260 873 years of life (IC 95% 208 180–347 023) were lost due to RSV bronchiolitis in Colombian children under 2 years. The estimated rate was 20 DALYs / 1000 person-year (95% CI 16–27).CONCLUSION This is the first report estimating the impact of RSV bronchiolitis morbidity and mortality in Colombia. The findings of the present study suggest that the actual burden and cost of bronchiolitis due to RSV is high. Prevention strategies to reduce morbidity associated with RSV infection should be encouraged in our country.
Cecum methylome response to Salmonella enterica serovar Enteritidis infection in chicken through whole genome bisulfite sequencing
Authors: Wang Yuanmei , Liu Liying , Li Min , Lin Lili , Su Pengcheng , Tang Hui , Fan Xinzhong , LI Xianyao 
Published: May 2020

Abstract
Background: Salmonella enterica serovar Enteritidis (SE) is one of the pathogenic bacteria, which affects poultry production and poses severe threat to public health. Chicken meat and egg are the main source of SE. DNA methylation, an important epigenetic modification, involves in regulatory processes including gene expression, chromatin structure and genomic imprinting. To understand the methylation regulation in response to SE inoculation in chicken, the genome-wide DNA methylation profile following SE inoculation was analyzed through whole genome bisulfite sequencing in the current study. Results: There were 185,362,463 clean reads and 126,098,724 unique reads in the control group, and 180,530,750 clean Reads, 126,782,896 unique reads in the inoculated group. We found that the methylation density in gene body was higher than that in the upstream and downstream regions of gene. There were 8,946 differentially methylated genes (3,639 hypo-methylated genes, 5,307 hyper-methylated genes) obtained between inoculated and control groups. Methylated genes were mainly enriched in immune-related Gene Ontology (GO) terms and metabolic process terms. Cytokine-cytokine receptor interaction, TGF-beta signaling pathway, FoxO signaling pathway, Wnt signaling pathway and several metabolism-related pathways were significantly enriched. The density of differentially methylated cytosines in miRNAs was the highest. HOX genes were widely methylated and mainly distributed in Chr2 and 7. Conclusions: We firstly analyzed the genome-wide DNA methylation in the response to SE inoculation in chicken. SE inoculation promoted the DNA methylation in chicken cecum and caused methylation alteration in immune- and metabolic- related genes. Wnt signal pathway, miRNAs and HOX gene family may play a crucial role in the methylation regulation of SE infection in chicken. The findings herein will deepen the understanding of epigenetic regulation in the response to SE inoculation in chicken.
Association of interleukin 2, interleukin 12, and interferon-γ with intervertebral disc degeneration in Iranian population
Authors: Hanaei Sara , Abdollahzade Sina , Sadr Maryam , Mirbolouk Mohammad Hossein , Fattahi Ehsan , Khoshnevisan Alireza , Rezaei Nima 
Published: May 2020

Abstract
Background: Intervertebral disc degeneration (IVDD) is an age-related degenerative disease, presenting low back pain or radicular pain in a variety of severity. As the inflammatory changes in discs, the inflammatory and anti-inflammatory cytokines, as well as their respective genes, have been proposed to play role in pathophysiology of disease. This study has been conducted to elucidate the role of IL-2, IL-12, and IFN-γ single nucleotide polymorphisms (SNP) in this disease.Method: 76 patients diagnosed with IVDD and 140 healthy subjects who have complied with eligibility criteria have been included. A total volume of 5cc peripheral blood of each participant has been used for investigating the IL-2 +166G/T, IL-2 -330G/T, IL-12 -1188A/C, and IFN-γ +847A/T single nucleotide polymorphisms (SNP) through PCR-SSP method.Results: The ‘TG’ and ‘TT’ genotypes of IL-2 -330G/T polymorphism have been significantly more common among patients and healthy controls respectively and therefore were associated with disease. The ‘GT’ and ‘TT’ haplotypes of IL-2, comprised of -330G/T, and +166G/T, have been also more common among patients and controls respectively.Conclusion: This study has indicated significant role of IL-2 genotypes and haplotypes in IVDD, as they have been differently distributed in cases and controls. Therefore, alteration in IL-2 gene structure could play an important role in pathophysiology of IVDD through alteration of its function.
Psychological correlates of COVID-19 pandemic in the Austrian population
Authors: Traunmüller Claudia , Stefitz Rene , Gaisbachgrabner Kerstin , Schwerdtfeger Andreas 
Published: May 2020

Abstract
Background: COVID-19 poses the greatest challenge for the entire world since the Second World War. Governments are forced to define strict measures to avoid the spreading of the virus, which may further impose psychological burden for the majority of the population. The aim of this study was to evaluate the psychological distress in Austria during the initial stage of the COVID-19 outbreak. Methods: From 25 March to 3 April 2020, an anonymous online survey was conducted. Target group included all members of the Austrian population older than 15 years. The survey addressed the following areas (1) and sociodemographic data, (2) physical and mental health; (3) knowledge and concerns about COVID-19; (4) contact with infected people; (5) prevention efforts; (6) need for further information. The Impact of Event Scale-Revised (IES-R) and the Depression, Anxiety and Stress Scale (DASS-21) were used to assess mental health. Analyses were based on 4,126 individuals (74% female, age: M = 38.68, SD = 13.36). Results: 43.3% rated the psychological impact as moderate (5.6%) or severe (37.7%). 26.5% reported moderate (13.3%) to severe (13.2%) depression; 20.3% moderate (8.9%) to severe (11.4%) anxiety and 21.2% reported to suffer from moderate (10.5%) or severe stress (10.7%). Being female, higher age, lower levels of education, concern about family members, internet as main source of information, student or pupil status, poor self-rated health, and the attitude that “there is too much unnecessary worry” were signiﬁcantly associated with higher psychological burden. Protective factors were the possibility to work in home office, frequent (indirect) contact with family or friends, the availability of virus-specific information, confidence in the diagnosis capability, and physical activity during the crisis.Conclusion: This study is among the first in Europe on the psychological correlates of the COVID-19 pandemic. 37.7% of the Austrian study population reported a severe psychological impact on the event and 1 in 10 is considered to suffer from severe depression, anxiety or stress. The present findings inform about the identification of protective factors, psychologically vulnerable groups and may guide the development of psychological interventions.