bluuebunny/crossref_metadata_2025_split huggingface dataset:
LAMININ STIMULATES EXPRESSION OF TWO MITOCHONDRIAL PROTEINS DURING NEURITE OUTGROWTH
Authors: Weeks Benjamin S. , Burbelo Peter , Jucker Mathias , Weiner Matthew A. , Roque Eva , Kleinman Hynda K. 
Published: July 2002

AbstractDifferential hybridization was utilized with mRNA from NG108‐15 cells cultured on either tissue culture plastic or laminin for 4 hr to identify genes whose mRNA was increased by laminin, a potent stimulator of neurite outgrowth. Two of the 16 laminin‐induced clones, cytochrome b and chargerin II, are mitochondrial proteins. Northern blotting confirmed that laminin increased the mRNA levels of cytochrome b and chargerin II several fold. Antibody to chargerin II stained both processes and cell bodies of the cerebellar Purkinje cells and localized in the mitochondria of NG108‐15 cells, which also showed increased protein levels in the presence of laminin. In addition, higher levels of chargerin II protein were detected in the newborn brain compared to the adult. However, inhibitors of mitochondrial protein synthesis did not affect laminin‐mediated neurite outgrowth. These data suggest that the increased synthesis of mitochondrial enzymes observed with laminin treatment may not be necessary for the formation of neurites.
A validation study of maternal self reports of obstetrical complications: implications for health surveys
Authors: Seoane G , Castrillo M , O'Rourke K 
Published: July 2002

AbstractObjective: The purpose of this study was to determine the validity of maternal self reports of obstetrical complications, which are commonly used in estimating the prevalence of complications. These estimates vary greatly between countries and could either reflect true differences or reporting bias. Methods: A cross‐sectional study was conducted among 1027 women in two Bolivian maternity hospitals. A questionnaire recorded mothers' perceptions of obstetrical complications while hospital medical records and physical examinations established their clinical condition. Sensitivity, specificity, predictive values, and percent agreement were obtained for obstetrical conditions. Results: In general, women's reports of obstetrical complications did not match medical diagnoses. The highest agreement was obtained for reporting eclampsia, with less agreement for labor disorders, postpartum hemorrhage and malpresentation. Conclusions: Maternal self reports in this study did not provide a valid estimate of the prevalence of obstetrical complications. Health surveys based on maternal self reports must be interpreted with consideration of this limitation.
Service quality along the supply chain: implications for purchasing
Authors: Stanley L.L. , Wisner J.D. 
Published: July 2002

AbstractThe overall objective of this research was to explore the association between implementation of cooperative purchasing/supplier relationships, internal service quality, and an organization’s ability to provide quality products and services to its customers. Specifically, purchasing‐related factors influencing internal and external product and service quality were identified from the literature and an internal service quality model was developed and then tested using empirical data. Survey data were collected from 118 US purchasing executives in a wide range of industries. The findings from this study indicate the existence of strong positive relationships between implementation of cooperative purchasing/supplier relationships, internal service quality, and the service and product quality provided to external customers. Additionally, the key role of purchasing in the integration and communication of quality expectations and quality performance throughout the firm is demonstrated.
Effect of service supplier performance on satisfaction and loyalty of store managers in the fast food industry
Authors: Stank Theodore P , Goldsby Thomas J , Vickery Shawnee K 
Published: July 2002

AbstractThe paper presents research that examines the complex relationship between service quality and profitability in service operations. Specifically, the effects of operational performance and relational performance on customer satisfaction and loyalty in the unique context of industrial services are examined. A model of service performance effects on customer satisfaction and loyalty is introduced and tested in the fast food service industry using customer perceptions of provider performance rather than relying on providers' self‐reported performance indicators. The research revealed that the benefit of establishing customer relationships emerges from the enhanced insight the supplier is able to gain regarding customer needs and wants. Upon learning of these needs and wants, the service provider can focus on operational means of meeting the needs and wants, and doing so in the lowest cost manner.
Predictability of dropout in unipolar depressed outpatients
Authors: Hoencamp E , Haffmans PMJ , Duivenvoorden HJ 
Published: July 2002

SummaryIn a three phase sequential treatment strategy study involving 119 depressed outpatients, a total of 31 patients (26.7%) stopped treatment prematurely due to side-effects (21/31), aggravation of symptoms (3/31), non-compliance (4/31), and non-treatment related events (3/31). At baseline, no significant differences were found on sociodemographic and psychiatric data between patients who did or did not drop out. As predictors of dropout eight domains of data concerning psychiatric history, premorbid history, symptomatology, personality, and social adjustment were used. Using a logistic linear regression analysis, only three variables were related to dropout. Patients with a history of alcohol use or poor social functioning according to axis V of the Diagnostic and Statistical Manual (DSM)-III-R had a higher chance to drop out, while patients with a sleep disturbance according to the Symptom Checklist (SCL)-90 had a smaller chance to drop out.
Up‐regulation of ‘clearance’ receptors in patients with chronic heart failure: a possible explanation for the resistance to biological effects of cardiac natriuretic hormones
Authors: Andreassi Maria Grazia , Del Ry Silvia , Palmieri Cataldo , Clerico Aldo , Biagini Andrea , Giannessi Daniela 
Published: July 2002

AbstractBackground:Three specific receptors for the cardiac natriuretic peptide system have been identified to date. Down‐regulation of the biologically active binding sites (i.e. NPR‐A and NPR‐B) could explain the blunted response to cardiac natriuretic hormones observed in heart failure (HF), but not the increased metabolic clearance rate. Variations in the ratio between biological and clearance (NPR‐C) receptors in target tissue may explain this increase.Aim:The aim of this study was to investigate the regulation of NPR‐C receptors on platelets, in patients with HF.Methods:Eighteen patients with HF (NYHA class: I–II, n ‐ 8; III–IV, n ‐ 10) and 18 age‐matched healthy subjects were studied. The affinity constant (Kd) and density (Bmax) of binding sites were derived by saturation assays on platelet suspensions using 125I‐ANP as radioligand.Results:Bmax increased as a function of the severity of disease: 21.3±3.3 fmol/109 cells in class III–IV, 11.7±2.2 in class I–II, and 11.6±1.1 in controls, respectively (P ‐ 0.0179 for class III–IV vs. controls and P ‐ 0.0451 vs. NYHA I–II).Conclusions:The increase in density of ‘clearance’ receptors in severe HF is theoretically consistent with the reduction in cardiac natriuretic peptide biological activity, as well as the increase in metabolic clearance rate. This suggests that clearance receptor blockade may be of potential therapeutic value in HF.
Wide‐open conformation of UDP‐MurNc‐tripeptide ligase revealed by the substrate‐free structure of MurE from Acinetobacter baumannii
Authors: Jung Kyoung Ho , Kim Yeon‐Gil , Kim Chang Min , Ha Hyun Ji , Lee Chang Sup , Lee Jun Hyuck , Park Hyun Ho 
Published: November 2020

MurE ligase catalyzes the attachment of meso‐diaminopimelic acid to the UDP‐MurNAc‐l‐Ala‐d‐Glu using ATP and producing UDP‐MurNAc‐l‐Ala‐d‐Glu‐meso‐A2pm during bacterial cell wall biosynthesis. Owing to the critical role of this enzyme, MurE is considered an attractive target for antibacterial drugs. Despite extensive studies on MurE ligase, the structural dynamics of its conformational changes are still elusive. In this study, we present the substrate‐free structure of MurE from Acinetobacter baumannii, which is an antibiotic‐resistant superbacterium that has threatened global public health. The structure revealed that MurE has a wide‐open conformation and undergoes wide‐open, intermediately closed, and fully closed dynamic conformational transition. Unveiling structural dynamics of MurE will help to understand the working mechanism of this ligase and to design next‐generation antibiotics targeting MurE.
Evaluation of cine imaging during multileaf collimator and gantry motion for real‐time magnetic resonance guided radiation therapy
Authors: Kielbasa Jerrold E. , Meeks Sanford L. , Kelly Patrick , Willoughby Twyla R. , Zeidan Omar , Shah Amish P. 
Published: November 2020

AbstractPurposeReal‐time magnetic resonance guided radiation therapy (MRgRT) uses 2D cine imaging for target tracking. This work evaluates the percent image uniformity (PIU) and spatial integrity of cine images in the presence of multileaf collimator (MLC) and gantry motion in order to simulate sliding window and volumetric modulated arc therapy (VMAT) conditions.MethodsPercent image uniformity and spatial integrity of cine images were measured (1) during MLC motion, (2) as a function of static gantry position, and (3) during gantry rotation. PIU was calculated according to the ACR MRI Quality Control Manual. Spatial integrity was evaluated by measuring the geometric distortion of 16 measured marker positions (10 cm or 15.225 cm from isocenter).ResultsThe PIU of cine images did not vary by more than 1% from static linac conditions during MLC motion and did not vary by more than 3% during gantry rotation. Banding artifacts were present during gantry rotation. The geometric distortion in the cine images was less than 0.88 mm for all points measured throughout MLC motion. For all static gantry positions, the geometric distortion was less than 0.88 mm at 10 cm from isocenter and less than 1.4 mm at 15.225 cm from isocenter. During gantry rotation, the geometric distortion remained less than 0.92 mm at 10 cm from isocenter and less than 1.60 mm at 15.225 cm from isocenter.ConclusionDuring MLC motion, cine images maintained adequate PIU, and the geometric distortion of points within 15.225 cm from isocenter was less than the 1 mm threshold necessary for real‐time target tracking and gating. During gantry rotation, PIU was negatively affected by banding artifacts, and spatial integrity was only maintained within 10 cm from isocenter. Future work should investigate the effects imaging artifacts have on real‐time target tracking during MRgRT.
CD8+ T cell subpopulations and pro‐inflammatory cytokines in neuromyelitis optica spectrum disorder
Authors: Shi Ziyan , Qiu Yuhan , Zhao Zhengyang , Wen Dingke , Chen Hongxi , Du Qin , Zhang Ying , Wang Jiancheng , Yan Chao , Yang Mu , Zhou Hongyu 
Published: November 2020

AbstractObjectiveOur study aimed to investigate circulating CD8+ T cell subpopulations and pro‐inflammatory cytokines in the neuromyelitis optica spectrum disorder (NMOSD).MethodsA total of 121 peripheral blood samples were obtained from 57 patients with NMOSD, 34 patients with multiple sclerosis (MS), and 30 sex‐ and age‐matched healthy controls (HCs) for detection of CD8+ T cell subpopulations, including phenotypes of naïve (TN, CD62LhiCD45RO‐), effector/memory (TE/M, CD62LloCD45RO+), memory precursor (TMP, CD127hiKLRG1lo), and short lived effector (TSLEC, CD127loKLRG1hi). In addition, 36 samples from 18 NMOSD, 12 MS, and 6 sex‐ and age‐matched HCs for detecting pro‐inflammatory cytokines (IFNγ and TNFα) using flow cytometry.ResultsCompared with HCs, we found significantly reduced CD8+ TN and increased CD8+ TE/M in both NMOSD and MS，while decreased CD8+ TMP was only observed in NMOSD. Patients treated with immunotherapy were associated with increased CD8+ TN and decreased CD8+ TE/M in NMOSD. Moreover NMOSD cohort showed significant higher proportions of IFNγ+CD8+ T cells and proportions of TNFα+CD8+ T cells than HC and MS cohorts. On the contrary, obviously decreased IFNγ and TNFα were found in NMOSD patients treated with immunotherapy. Furthermore, Multivariate linear regression analyses revealed that age was negatively correlated with CD8+ TN and TMP, and positively associated with TSLEC; however, sex, EDSS scores and disease phase were not significantly associated with CD8+ T subpopulations.InterpretationThis current study provides an evidence that circulating CD8+ T cell with abnormal subpopulations and increased pro‐inflammatory were associated with pathogenesis of autoimmune demyelinating disease of CNS, especially in NMOSD.
Assessing conservation attitudes and behaviors of Congolese children neighboring the world's first bonobo (Pan paniscus) release site
Authors: Bowie Aleah , Walker Kara , Bunnell Gabrielle , Morel Dominique , Minesi Fanny , Belais Raphael , Hare Brian 
Published: November 2020

AbstractPoaching and habitat destruction in the Congo Basin threaten African great apes including the bonobo (Pan paniscus), chimpanzees (Pan troglodytes), and gorillas (Gorilla spp.) with extinction. One way to combat extinction is to reintroduce rescued and rehabilitated apes and repopulate native habitats. Reintroduction programs are only successful if they are supported by local populations. Ekolo ya Bonobo, located in Equateur province of the Democratic Republic of Congo (DRC), is the world's only reintroduction site for rehabilitated bonobos. Here we assess whether children, of the Ilonga‐Pôo, living adjacent to Ekolo ya Bonobo demonstrate more pro‐ape conservation attitudes than children living in, Kinshasa, the capital city. We examined children's attitudes toward great apes because children are typically the focus of conservation education programs. We used the Great Ape Attitude Questionnaire to test the Contact Hypothesis, which posits that proximity to great ape habitat influences pro‐conservation attitudes toward great apes. Ilonga‐Pôo children who live in closer contact with wild bonobos felt greater responsibility to protect great apes compared to those in Kinshasa who live outside the natural habitat of great apes. These results suggest that among participants in the DRC, spatial proximity to a species fosters a greater sense of responsibility to protect and conserve. These results have implications for the successful implementation of great ape reintroduction programs in the Congo Basin. The data analyzed in this study were collected in 2010 and therefore provide a baseline for longitudinal study of this reintroduction site.
Gene‐ and age‐informed screening for preclinical Alzheimer's disease trials
Authors: Spencer Barbara E. , Digma Leonardino A. , Jennings Robin G. , Brewer James B. ,   for the Alzheimer's Disease Neuroimaging Initiative and the A4 Study Team
Published: November 2020

AbstractIntroductionElevated β‐amyloid is used to enroll individuals into preclinical Alzheimer's disease trials, but the screening process is inefficient and expensive. Novel enrichment methods are needed to improve efficiency of enrollment.MethodsAlzheimer's disease incidence rates and a polygenic hazard score were used to create a gene‐ and age‐defined ADAge. An ADAge cutpoint was chosen to optimally predict β‐amyloid positivity among clinically normal Alzheimer's Disease Neuroimaging Initiative participants and applied to an independent Alzheimer's Disease Research Center validation cohort. The impact of ADAge enrichment on screening costs was evaluated in the Anti‐Amyloid Treatment in Asymptomatic Alzheimer's Disease trial data.ResultsIn the validation cohort, the ADAge‐enriched sample had a higher proportion of individuals with elevated β‐amyloid (difference [95% CI] 0.19[0.07 to 0.33]) than the unenriched sample. ADAge enrichment lowered screening costs by $4.41 million (31.00%) in the real‐world clinical trial scenario.DiscussionADAge enrichment provides for a more efficient and cost‐effective means to enroll clinically normal individuals with elevated β‐amyloid in clinical trials.
Optimal age and outcome measures for Alzheimer's disease prevention trials in people with Down syndrome
Authors: Hithersay Rosalyn , Baksh R. Asaad , Startin Carla M. , Wijeratne Peter , Hamburg Sarah , Carter Ben ,   The LonDownS Consortium, Strydom Andre 
Published: November 2020

AbstractIntroductionPeople with Down syndrome (DS) typically develop Alzheimer's disease (AD) neuropathology before age 40, but a lack of outcome measures and longitudinal data have impeded their inclusion in randomized controlled trials (RCTs).MethodsCohort study. Event‐based and dose‐response Emax models were fitted to longitudinal cognitive data, to stage AD and determine the earliest ages of decline. Results informed sample size estimations for hypothetical RCTs of disease‐modifying treatments that reduced decline by 35% or 75%.ResultsSeventy‐five percent of participants progressed or remained stable in the AD staging model; effect sizes varied by age group and tests. Varied treatment effects could be detected with 50‐200 people per arm when using sensitive cognitive outcome measures and targeting recruitment to ages 36 to 45 years.DiscussionEfficient RCTs of AD preventative treatments can be conducted in the DS population using sensitive outcome measures to monitor early decline. Dose‐response models could help tailor future RCTs.
Ruthenium (II) Catalyzed C(sp2)−H Bond Alkenylation of 2‐Arylbenzo[d]oxazole and 2‐Arylbenzo[d]thiazole with Unactivated Olefins
Authors: Pipaliya Bhavin V. , Seth Kapileswar , Chakraborti Asit K. 
Published: November 2020

AbstractFunctionalization of the bio‐relevant heterocycles 2‐arylbenzo[d]oxazole and 2‐arylbenzo[d]thiazole has been achieved through Ru(II)‐catalyzed alkenylation with unactivated olefins leading to selective formation of the mono‐alkenylated products. This approach has a broad substrate scope with respect to the coupling partners, affords high yields, and works for gram scale synthesis using a readily available Ru‐based catalyst. Mechanistic studies reveal a C−H activation pathway for the dehydrogenative coupling leading to the alkenylation. However, the results of the ESI‐MS‐guided deuterium kinetic isotope effect studies indicate that the C−H activation stage may not be the rate‐determining step of the reaction. The use of a radical scavenging agent such as TEMPO did not show any detrimental effect on the reaction outcome, eliminating the possibility of the involvement of a free‐radical pathway.
Novel enzyme‐based electrochemical and colorimetric biosensors for tetracycline monitoring in milk
Authors: Besharati Maryam , Tabrizi Mahmoud Amouzadeh , Molaabasi Fatemeh , Saber Reza , Shamsipur Mojtaba , Hamedi Javad , Hosseinkhani Saman 
Published: November 2020

AbstractRecently, there has been a growing demand to develop portable devices for the fast detection of contaminants in food safety, healthcare, and environmental fields. Herein, two biosensing methods were designed by the use of nicotinamide adenine dinucleotide phosphate (NAD(P)H)‐dependent TetX2 enzyme activity and thionine as an excellent electrochemical and colorimetric mediator/probe to monitor tetracycline (TC) in milk. The nanoporous glassy carbon electrode (NPGCE) modified with polythionine was first prepared by electrochemically and then TetX2 was immobilized onto the NPGCE using polyethyleneimine. The prepared biosensor provided a high electrocatalytic response toward NAD(P)H by significantly reducing its overpotential. The proposed biosensor exhibited a detection limit of 40 nM with a linear range of 0.1–0.8 μM for TC determination. Besides, the thionine probe was used to develop a novel colorimetric assay using a simple enzymatic color reaction within a few minutes. The limit of detection for TC was experimentally achieved as 60 nM, which was lower than the safety levels established by the World Health Organization (225 nM). The correlation between change in the color of the solution and the concentration of TC was used for quality control of milk samples, as confirmed by the standard high‐performance liquid chromatography method. The results show the great potential of the proposed assays as portable instruments for on‐site TC measurements.
A novel full symmetric diarylethene‐based ratiometric fluorescent sensor for lysine and the application for a logic circuit
Authors: Zhu Zifan , Wang Yuesong , Ding Haichang , Fan Congbin , Tu Yayi , Liu Gang , Pu Shouzhi 
Published: November 2020

AbstractA novel diarylethene‐based ratiometric fluorescent sensor with full symmetric structure, 1o, was designed and synthesized successfully. 1o could identify lysine (Lys) with high selectivity and sensitivity and the fluorescence emission peak was red shifted 85 nm upon addition of Lys, which could realize ratio recognition. It exhibited excellent anti‐interference performance in the presence of various amino acids in CH3CN/H2O (7/3, v/v) solution. Moreover, the limit of detection of 1o to Lys could reach 0.019 μM based on a good linear range of 0–40 μM. In addition, the fluorescence emission intensity of 1o could be turned off/on by ultraviolet/visible light due to the special structure of diarylethene. A logic circuit was designed with three inputs. The ratiometric fluorescent sensor 1o could be as a new tool and provide a new method for detection of Lys.
Systemic circular business model application at the company, supply chain and society levels—A view into circular economy native and adopter companies
Authors: Rovanto Ida K. , Bask Anu 
Published: November 2020

AbstractThis study contributes to the conceptualization of a sustainable circular business model (CBM), the application of the circular economy (CE) by companies. The study utilized a three‐level framework adapted from the CE literature with company (micro), supply chain (meso) and society (macro) levels. This multiple‐case study in the textile industry included two types of companies operating in the CE: companies founded on the sustainable principles of a CE (natives) and companies transitioning to a CE from the linear economy (adopters). The findings show that the adopters emphasized long‐term economic sustainability on a company level and implemented CE elements to varying degrees on all three levels. The natives pursued business decisions from environmental and social sustainability standpoints, and the three levels were integral in their systemic approach to a CE. The study highlights two key claims: established operational structures and economic volition hindered adopters in their systemic CE implementation, and an integral part of the CBM for natives was a proactive approach towards the society level.
Transformable Decentral Production for Local Economies with Minimized Carbon Footprint
Authors: Pannok Maik , Finkbeiner Marco , Fasel Henrik , Riese Julia , Lier Stefan 
Published: November 2020

AbstractDue to high energy‐intensive processes and a dependence on carbon‐based materials, the process industry plays a major role in climate change. Therefore, the substitution of fossil resources by bio‐based resources is indispensable. This leads to challenges arising from accompanying changes of the type, amount and location of resources. At the same time, transformable production systems are currently in the focus of research addressing the required flexibility. These systems which consist of modular production and logistics units offer the possibility to adapt flexibly in volatile conditions within dynamic supply chains. Hence, this work compiles elements for environmental sustainability, which minimize the carbon footprint in the process industry: transformable production systems, the utilization of bio‐based resources, carbon dioxide and renewable energy as well as the application of these elements in decentral production networks. Finally, possible use cases are determined based on the combination of these elements through a multi‐criteria analysis.