bluuebunny/crossref_metadata_2025_split huggingface dataset:
The blood-brain barrier is disrupted in Machado-Joseph disease/spinocerebellar ataxia type 3: evidence from transgenic mice and human post-mortem samples
Authors: Duarte Lobo Diana , Nobre Rui Jorge , Oliveira Miranda Catarina , Pereira Dina , Castelhano João , Sereno José , Koeppen Arnulf , Castelo-Branco Miguel , Pereira de Almeida Luís 
Published: August 2020

AbstractBlood-brain barrier (BBB) disruption is a common feature in neurodegenerative diseases. However, BBB integrity has not been assessed in spinocerebellar ataxias (SCAs) such as Machado-Joseph disease/SCA type 3 (MJD/SCA3), a genetic disorder, triggered by polyglutamine-expanded ataxin-3. To investigate that, BBB integrity was evaluated in a transgenic mouse model of MJD and in human post-mortem brain tissues.Firstly, we investigated the BBB permeability in MJD mice by: i) assessing the extravasation of the Evans blue (EB) dye and blood-borne proteins (e.g fibrinogen) in the cerebellum by immunofluorescence, and ii) in vivo Dynamic Contrast Enhanced-Magnetic Resonance Imaging (DCE-MRI). The presence of ataxin-3 aggregates in brain blood vessels and the levels of tight junction (TJ)-associated proteins were also explored by immunofluorescence and western blotting. Human brain samples were used to confirm BBB permeability by evaluating fibrinogen extravasation, co-localization of ataxin-3 aggregates with brain blood vessels and neuroinflammation.In the cerebellum of the mouse model of MJD, there was a 5-fold increase in EB accumulation when compared to age-matched controls. Moreover, vascular permeability displayed a 13-fold increase demonstrated by DCE-MRI. These results were validated by the 2-fold increase in fibrinogen extravasation in transgenic animals comparing to controls. Interestingly, mutant ataxin-3 aggregates were detected in cerebellar blood vessels of transgenic mice, accompanied by alterations of TJ-associated proteins in cerebellar endothelial cells, namely a 29% decrease in claudin-5 oligomers and a 10-fold increase in an occludin cleavage fragment. These results were validated in post-mortem brain samples from MJD patients as we detected fibrinogen extravasation across BBB, the presence of ataxin-3 aggregates in blood vessels and associated microgliosis.Altogether, our results prove BBB impairment in MJD/SCA3. These findings contribute for a better understanding of the disease mechanisms and opens the opportunity to treat MJD with medicinal products that in normal conditions would not cross the BBB.
Increased levels of Stress-inducible phosphoprotein-1 accelerates amyloid-β deposition in a mouse model of Alzheimer’s disease
Authors: Lackie Rachel E. , Marques-Lopes Jose , Ostapchenko Valeriy G. , Good Sarah , Choy Wing-Yiu , van Oosten-Hawle Patricija , Pasternak Stephen H. , Prado Vania F. , Prado Marco A. M. 
Published: August 2020

AbstractMolecular chaperones and co-chaperones, which are part of the protein quality control machinery, have been shown to regulate distinct aspects of Alzheimer’s Disease (AD) pathology in multiple ways. Notably, the co-chaperone STI1, which presents increased levels in AD, can protect mammalian neurons from amyloid-β toxicity in vitro and reduced STI1 levels worsen Aβ toxicity in C. elegans. However, whether increased STI1 levels can protect neurons in vivo remains unknown. We determined that overexpression of STI1 and/or Hsp90 protected C. elegans expressing Aβ(3–42) against Aβ-mediated paralysis. Mammalian neurons were also protected by elevated levels of endogenous STI1 in vitro, and this effect was mainly due to extracellular STI1. Surprisingly, in the 5xFAD mouse model of AD, by overexpressing STI1, we find increased amyloid burden, which amplifies neurotoxicity and worsens spatial memory deficits in these mutants. Increased levels of STI1 disturbed the expression of Aβ-regulating enzymes (BACE1 and MMP-2), suggesting potential mechanisms by which amyloid burden is increased in mice. Notably, we observed that STI1 accumulates in dense-core AD plaques in both 5xFAD mice and human brain tissue. Our findings suggest that elevated levels of STI1 contribute to Aβ accumulation, and that STI1 is deposited in AD plaques in mice and humans. We conclude that despite the protective effects of STI1 in C. elegans and in mammalian cultured neurons, in vivo, the predominant effect of elevated STI1 is deleterious in AD.
Is there a relationship between malocclusion and bullying? A systematic review
Authors: Tristão Sylvia Karla P. C. , Magno Marcela B. , Pintor Andréa Vaz Braga , Christovam Ilana F. O. , Ferreira Daniele Masterson T. P. , Maia Lucianne Cople , de Souza Ivete Pomarico Ribeiro 
Published: August 2020

Abstract
Background
Malocclusion is a highly prevalent public health problem, and several studies have shown its negative correlation with quality of life, self-esteem, and social perceptions. However, its association with bullying is still controversial.

Objectives
To evaluate the relationship between malocclusion and bullying in children and adolescents.

Search methods
The databases used for the electronic researches were PubMed, Scopus, Lilacs/BBO, Web of Science, and Cochrane Library. Grey literature was reviewed through Open Grey literature with no language or date restrictions. Selection criteria, based on the PECO strategy, were considered eligible observational studies that included schoolchildren or adolescents (P) with malocclusion (E), compared to those with normal occlusion (C), in which the relationship between malocclusion and bullying was determined (O).

Data collection and analysis
Risk of bias evaluation was made for the qualitative synthesis by the Fowkes and Fulton criteria. Data regarding the age of participants and types of malocclusion and of bullying were extracted among other reported data. The quality of the evidence analyzed was evaluated through the GRADE approach.

Results
From 2744 articles identified in databases, nine met the eligibility criteria and were included in present systematic review, of which two studies were judged with methodological soundness. The quality of the evidence was classified as very low due to very serious problems for “risk of bias” and “other considerations” and serious problems of “indirectness”. The age of participants ranged from 9 to 34 years considering a cohort study, with a bullying recalling perspective. Malocclusion was both evaluated by researchers and self-reported by participants addressing dentofacial characteristics mostly related to the incisors relationship. All studies evaluated the verbal type of bullying, while 3 also considered physical type. Both types were reported as related to malocclusion, although the results showed that extreme maxillary overjet (> 4 mm, > 6 mm, > 9 mm), extreme deep overbite, and having space between anterior teeth or missing teeth were the types of malocclusion with the strongest relations to bullying.

Conclusions
With very low certainty of evidence, the results of this systematic review suggest that conspicuous extreme malocclusion may be related to the occurrence of bullying among children and adolescents.
Investigation of the effects of P1 on HC-pro-mediated gene silencing suppression through genetics and omics approaches
Authors: Hu Sin-Fen , Wei Wei-Lun , Hong Syuan-Fei , Fang Ru-Ying , Wu Hsin-Yi , Lin Pin-Chun , Sanobar Neda , Wang Hsin-Ping , Sulistio Margo , Wu Chun-Ta , Lo Hsiao-Feng , Lin Shih-Shun 
Published: August 2020

Abstract
Background
Posttranscriptional gene silencing (PTGS) is one of the most important mechanisms for plants during viral infection. However, viruses have also developed viral suppressors to negatively control PTGS by inhibiting microRNA (miRNA) and short-interfering RNA (siRNA) regulation in plants. The first identified viral suppressor, P1/HC-Pro, is a fusion protein that was translated from potyviral RNA. Upon infecting plants, the P1 protein itself is released from HC-Pro by the self-cleaving activity of P1. P1 has an unknown function in enhancing HC-Pro-mediated PTGS suppression. We performed proteomics to identify P1-interacting proteins. We also performed transcriptomics that were generated from Col-0 and various P1/HC-Pro-related transgenic plants to identify novel genes. The results showed several novel genes were identified through the comparative network analysis that might be involved in P1/HC-Pro-mediated PTGS suppression.

Results
First, we demonstrated that P1 enhances HC-Pro function and that the mechanism might work through P1 binding to VERNALIZATION INDEPENDENCE 3/SUPERKILLER 8 (VIP3/SKI8), a subunit of the exosome, to interfere with the 5′-fragment of the PTGS-cleaved RNA degradation product. Second, the AGO1 was specifically posttranslationally degraded in transgenic Arabidopsis expressing P1/HC-Pro of turnip mosaic virus (TuMV) (P1/HCTu plant). Third, the comparative network highlighted potentially critical genes in PTGS, including miRNA targets, calcium signaling, hormone (JA, ET, and ABA) signaling, and defense response.

Conclusion
Through these genetic and omics approaches, we revealed an overall perspective to identify many critical genes involved in PTGS. These new findings significantly impact in our understanding of P1/HC-Pro-mediated PTGS suppression.
Group based emotion recognition from video sequence with hybrid optimization based recurrent fuzzy neural network
Authors: Sreenivas Velagapudi , Namdeo Varsha , Kumar E. Vijay 
Published: August 2020

AbstractGroup-based emotion recognition (GER) is an interesting topic in both security and social area. In this paper, a GER with hybrid optimization based recurrent fuzzy neural network is proposed which is from video sequence. In our work, by utilizing the Neural Network the emotion recognition (ER) is performed from group of people. Initially, original video frames are taken as input and pre-process it from multi user video data. From this pre-processed image, the feature extraction is done by Multivariate Local Texture Pattern (MLTP), gray-level co-occurrence matrix (GLCM), and Local Energy based Shape Histogram (LESH). After extracting the features, certain features are selected using Modified Sea-lion optimization algorithm process. Finally, recurrent fuzzy neural network (RFNN) classifier based Social Ski-Driver (SSD) optimization algorithm is proposed for classification process, SSD is used for updating the weights in the RFNN. Python platform is utilized to implement this work and the performance of accuracy, sensitivity, specificity, recall and precision is evaluated with some existing techniques. The proposed method accuracy is 99.16%, recall is 99.33%, precision is 99%, sensitivity is 99.93% and specificity is 99% when compared with other deep learning techniques our proposed method attains good result.
Traditional food knowledge of Indonesia: a new high-quality food dataset and automatic recognition system
Authors: Wibisono Ari , Wisesa Hanif Arief , Rahmadhani Zulia Putri , Fahira Puteri Khatya , Mursanto Petrus , Jatmiko Wisnu 
Published: August 2020

AbstractTraditional food knowledge (TFK) is an essential aspect of human life. In terms of sociocultural aspects, TFK is necessary to protect ancestral culture. In terms of health, traditional foods contain better and more natural ingredients compared to the ingredients of processed foods. Considering this background, in this study, data acquisition and automatic food recognition were performed for traditional food in Indonesia. The food images were captured in a professional mini studio. The food image data were captured under the same light intensity, camera settings, and shooting distance from the camera. The parameters were precisely measured and configured with a light intensity meter, adjustable lighting, and a laser distance measurement device. The data of 1644 traditional food images were successfully obtained in the data acquisition process. These images corresponded to 34 types of traditional foods, and 30–50 images were obtained for each type of food. The size of the raw food image data was 53 GB. The data were divided into sets for training, testing, and validation. An automatic recognition system was developed to classify the traditional food of Indonesia. Training was performed using several types of convolutional neural network (CNN) models such as Densenet121, Resnet50, InceptionV3, and Nasnetmobile. The evaluation results indicated that when using a high quality dataset, the automatic recognition system could realize satisfactory area under the receiver operating characteristics (AUROC) and high accuracy, precision, and recall values of more than 0.95.
Association between a history of depression and anti-müllerian hormone among late-reproductive aged women: the Harvard study of moods and cycles
Authors: Golenbock Samuel W. , Wise Lauren A. , Lambert-Messerlian Geralyn M. , Eklund Elizabeth E. , Harlow Bernard L. 
Published: August 2020

Abstract
Background
There is conflicting evidence regarding the association between a history of depression and risk of early menopause. In a cohort of premenopausal women, we investigated the association between depression history and ovarian reserve, as measured by anti-müllerian hormone (AMH).

Methods
The Harvard Study of Moods and Cycles (HSMC) was a prospective cohort study of women living in the Boston, MA metropolitan-area (1995–1999). Women aged 36–45 years at cohort entry (1995) were sampled from seven Boston metropolitan-area communities using census directories. We measured serum AMH in early-follicular phase venous blood specimens from 141 women with a Structured Clinical Interview for DSM-IV (SCID)-confirmed history of depression and 228 without such a history. We calculated prevalence ratios (PR) for the association between characteristics of depression history and low AMH (≤1.4 ng/mL), adjusting for several potential confounders.

Results
The prevalence of low AMH was similar among depressed (57.5%) and non-depressed (57.9%) women (Adjusted [Adj] PR = 0.90, 95% CI: 0.75, 1.08). Among depressed women, results were not appreciably different among those who had ever used antidepressants and those with comorbid anxiety. Modest inverse associations between depression and low AMH were seen among women aged 36–40 years (Adj PR = 0.75, 95% CI: 0.52, 1.09) and nulliparous women (Adj PR = 0.77, 95% CI: 0.59, 1.00). No dose-response association with greater duration or length of depressive symptoms was observed.

Conclusions
Overall, the prevalence of low AMH was similar for depressed and non-depressed women 36–45 years of age. Surprisingly, among younger and nulliparous women, those with a history of depression had a slightly reduced prevalence of low AMH relative to those without such a history. These results do not indicate reduced ovarian reserve among women with a history of depression.
AbobotulinumtoxinA using 2-mL dilution (500 U/2-mL) maintains durable improvement across multiple treatment cycles
Authors: Dashtipour Khashayar , Wietek Stefan , Rubin Bruce , Maisonobe Pascal , Bahroo Laxman , Trosch Richard 
Published: August 2020

Abstract
Background
Cervical dystonia (CD), the most common focal dystonia, is a chronic neurological movement disorder characterized by sustained involuntary contractions of the neck muscles, leading to abnormal postures. AbobotulinumtoxinA (aboBoNT-A) was approved in the US initially as a 500 U per 1-mL dilution and subsequently, as a 500 U/2-mL dilution (or 250 U/mL), thereby providing clinicians with more flexible dosing options to better meet individual patient needs. The objective of this open-label extension study was to evaluate the longer term safety and efficacy of repeat treatments with aboBoNT-A using 2-mL dilutions in adults with cervical dystonia.

Methods
Patients (N = 112) from a 12-week, double-blind lead-in study (NCT01753310) received up to three additional treatments of aboBoNT-A, with re-treatment every 12–16 weeks based on clinical judgment. Safety was assessed through treatment-emergent adverse events (TEAEs). The Toronto Western Spasmodic Torticollis Rating Scale (TWSTRS) total and subscale scores were measured at day 1 of each treatment cycle (C), 4 weeks after each treatment, and 12 weeks after the third treatment. Descriptive statistics were used for all analyses.

Results
In cycles 1, 2, 3, and 4, respectively, 35.7, 25.9, 30.2, and 22.8% of patients reported TEAEs. Dysphagia, muscular weakness, and neck pain were each reported by 10.7% of patients, over the full study duration. Mean TWSTRS total score decreased from 37.7 (SD 13.6 [C1, day 1]) to 30.1 (SD 12.8 [C3, week 12]). In each cycle, TWSTRS total and subscale scores decreased from day 1 to week 4 and increased between weeks 4 and 12, though the week 12 scores remained lower than day 1 scores.

Conclusion
Extended treatment of cervical dystonia with aboBoNT-A (up to 3 additional treatment cycles) using a 2-mL dilution is effective, with a positive risk-benefit profile.

Trial registration
ClinicalTrials.gov Identifier: NCT01753336. Registered 17 Dec 2012.
Endoscopic observation of the palisade vessels in Killian–Jamieson diverticulum was useful for diagnosis and surgical treatment: a case report
Authors: Kurahashi Yasunori , Hojo Yudai , Nakamura Tatsuro , Kumamoto Tsutomu , Nakanishi Yasutaka , Ishida Yoshinori , Shinohara Hisashi 
Published: August 2020

Abstract
Background
Killian–Jamieson diverticulum is a rare pharyngoesophageal diverticulum that arises below the cricopharyngeus muscle. Unlike the most common Zenker’s diverticulum, which requires cricopharyngeal and esophageal myotomy, diverticulectomy is sufficient for surgical treatment of Killian–Jamieson diverticulum. Thus, accurate preoperative diagnosis is indispensable for avoiding unnecessarily invasive surgery. Here, we report a case of Killian–Jamieson diverticulum in which endoscopic observation of the palisade vessels was useful for diagnosis and intraoperative endoscopy was effective in guiding surgical resection.

Case presentation
A 65-year-old woman complained of pharyngeal discomfort and increased coughing and was referred to our hospital with a diagnosis of a pharyngoesophageal diverticulum. Contrast esophagography and cervical computed tomography revealed a diverticulum measuring 3 cm in diameter on the left side of the cervix. The diverticulum was identified by endoscopy just below the palisade vessels, which represents the level of the upper esophageal sphincter, and was diagnosed as Killian–Jamieson diverticulum. She underwent diverticulectomy without cricopharyngeal and esophageal myotomy. After exposing the diverticulum under light from the endoscope and washing out the food residue inside endoscopically, the diverticulum was resected using the endoscope as a bougie so as not to narrow the esophagus. The postoperative course was uneventful, and she remains asymptomatic without recurrence or stenosis at 6 months after surgery.

Conclusions
Endoscopic observation of the palisade vessels in addition to esophagography can help diagnose Killian–Jamieson diverticulum and determine the optimal surgical procedure. Diverticulectomy can be performed intentionally and safely with the aid of intraoperative endoscopy.
Using meta-analysis of technique and timing to optimize corrective feedback for specific grammatical features
Authors: Schenck Andrew 
Published: August 2020

AbstractGrammar varies in semantic, morphosyntactic, and phonological complexity, which may influence what type of Corrective Feedback (CF) is effective. The present study was designed to investigate the best form of CF for each grammatical feature, in conjunction with associated variables such as learner proficiency level and L1 influence. Fifteen studies, all of which had English as a Foreign Language (EFL) learners with a Korean L1 and productive measures of speech or writing, were selected for study. Results suggest that grammatical features which are very similar to the L1 may benefit more from implicit reformulations (recasts). Explicit CF (direct and metalinguistic feedback) appears more useful for semantically and syntactically complex features such as the English article and past hypothetical conditional. Finally, research of proficiency level suggests that timely emphasis of specific grammatical features is needed in order for CF to be effective.
3D human pose estimation model using location-maps for distorted and disconnected images by a wearable omnidirectional camera
Authors: Miura Teppei , Sako Shinji 
Published: August 2020

AbstractWe address a 3D human pose estimation for equirectangular images taken by a wearable omnidirectional camera. The equirectangular image is distorted because the omnidirectional camera is attached closely in front of a person’s neck. Furthermore, some parts of the body are disconnected on the image; for instance, when a hand goes out to an edge of the image, the hand comes in from another edge. The distortion and disconnection of images make 3D pose estimation challenging. To overcome this difficulty, we introduce the location-maps method proposed by Mehta et al.; however, the method was used to estimate 3D human poses only for regular images without distortion and disconnection. We focus on a characteristic of the location-maps that can extend 2D joint locations to 3D positions with respect to 2D-3D consistency without considering kinematic model restrictions and optical properties. In addition, we collect a new dataset that is composed of equirectangular images and synchronized 3D joint positions for training and evaluation. We validate the location-maps’ capability to estimate 3D human poses for distorted and disconnected images. We propose a new location-maps-based model by replacing the backbone network with a state-of-the-art 2D human pose estimation model (HRNet). Our model is a simpler architecture than the reference model proposed by Mehta et al. Nevertheless, our model indicates better performance with respect to accuracy and computation complexity. Finally, we analyze the location-maps method from two perspectives: the map variance and the map scale. Therefore, some location-maps characteristics are revealed that (1) the map variance affects robustness to extend 2D joint locations to 3D positions for the 2D estimation error, and (2) the 3D position accuracy is related to the 2D locations relative accuracy to the map scale.
Changes in serum concentration of rilpivirine in an HIV-infected patient treated with a combination therapy of hemodialysis and peritoneal dialysis
Authors: Oshiro Nanako , Kohagura Kentaro , Tsuneyoshi Shoji , Tateyama Masao , Zamami Ryo , Uehara Hitoshi , Fujita Jiro , Ohya Yusuke 
Published: August 2020

Abstract
Background
To our knowledge, there are no preexisting reports concerning rilpivirine (RPV) removal by hemodialysis and peritoneal dialysis.

Case presentation
This study aimed to evaluate the effect of hemodialysis and peritoneal dialysis on plasma concentrations of RPV in a 45-year-old man infected with HIV and exhibiting end-stage renal disease (ESRD). The extraction ratio of RPV by hemodialysis was 4.5%. Plasma concentrations of RPV remained far above the protein-binding-adjusted inhibitory levels during a combination therapy of hemodialysis and peritoneal dialysis. Our results suggest minimal RPV removal via hemodialysis and peritoneal dialysis with no specific dosage adjustments required in an HIV-infected patient undergoing this combination therapy.

Conclusion
In conclusion, this study showed that RPV administered without dose adjustment resulted in steady-state plasma drug concentration in an HIV-infected patient treated with a combination therapy of hemodialysis and peritoneal dialysis.