bluuebunny/crossref_metadata_2025_split huggingface dataset:
Distribusi Spasial Logam Berat Besi (Fe) di Perairan Teluk Staring, Sulawesi Tenggara
Authors: Bahril Bahril , Armid Armid , Jabir Jabir , Takwir Amadhan , Rahim Abdul 
Published: December 2019

<table width="645" border="1" cellspacing="0" cellpadding="0"><tbody><tr><td valign="top" width="408"><p> </p><p>Study on spatial distribution of metal Fe in the coastal area of Staring Bay, Southeast Sulawesi has been carried out. This study aims to determine the spatial distributions of heavy Fe in the coastal area of Staring Bay. Sampling was conducted at 12 stations along the bay through the purposive sampling method. Determination of heavy metal Fe concentration was performed utilizing the <em>Atomic Absorption Spectrophotomet</em><em>ry</em><em> </em>(AAS). Spatial analysis was performed with IDW interpolation method using a software ArcGIS 10.3. The results showed that the concentration of metal Fe at 12 stations was in the range of 0.00187 ppm to 0.01296 ppm whereby the highest level was found at station 7 (mouth of the Laonti river). The spatial analysis in 12 stations confirmed that in the coastal areas of Woru-woru village (near the mouth of the Laonti river) to Gala Island and Wandahea village up to Intan Island have been contaminated by metal Fe ranged from 0.0106-0.0117 ppm and 0.0118-0.013 ppm, respectively. The contamination factor of metal Fe was moderate. Based on the values of CF, the highest contamination of heavy metal Fe is at station 7.</p><p> </p><p>Keywords: Spatial analysis, Fe, IDW, CF, Staring Bay</p><p class="BodyAbstract"> </p><p class="BodyAbstract"> </p><p>Telah dilakukan studi distribusi spasial logam Fe di perairan Teluk Staring, Sulawesi Tenggara. Penelitian ini bertujuan untuk menentukan sebaran spasial logam Fe di perairan Teluk Staring. Pengambilan sampel dilakukan 12 stasiun di perairan teluk dengan metode <em>purposive sampling</em>. Penentuan kadar logam Fe pada sampel air laut menggunakan <em>Atomic Absorption Spectrophotomet</em><em>ry</em><em> </em>(AAS). Analisis spasial dilakukan dengan metode interpolasi IDW menggunakan <em>software</em> ArcGIS 10.3. Hasil penelitian menunjukkan bahwa kadar logam Fe di 12 stasiun penelitian berkisar antara 0,00187-0,01296 ppm dengan kadar tertinggi berada pada stasiun 7 (muara Sungai Laonti). Hasil analisis spasial pada 12 stasiun menunjukkan bahwa di daerah pesisir Desa Woru-woru (dekat muara Sungai Laonti) hingga Pulau Gala dan Desa Wandahea hingga Pulau Intan telah terkontaminasi logam Fe dengan kisaran 0,0106-0,0117 ppm dan 0,0118-0,013 ppm. Faktor kontaminasi logam Fe bersifat moderat. Berdasarkan nilai CF, kontaminasi tertinggi oleh logam Fe terdapat pada stasiun 7.</p><p> </p><p>Kata kunci:<em> </em>Analisis spasial, Fe, IDW, CF, Teluk Staring</p><p> </p></td></tr></tbody></table>
DIVERSITY OF ENDOPHYTIC FUNGI IN THE ROOT, LEAF, STOLON AND PETIOLE OF ASIATIC PENNYWORT (Centella asiatica)
Authors: Susilowati Dwi Ningsih , Rakhmaniar Amelia , Radiastuti Nani , Roostika Ika 
Published: December 2019

<p>Endophytic fungi live in healthy tissues of many plants, including in medicinal plant such as Asiatic pennywort (<em>Centella asiatica</em>)<em>.</em> These fungi exist in different parts of the plant as symbionts. The study aimed to isolate endophytic fungi from various parts of Asiatic pennywort of Malaysia accession and characterize their nature. Three individual plants of Asiatic pennywort (3 months-old) were obtained from the Sringanis Medicinal Garden in Bogor. The endophytes were isolated on Malt Extract Agar. The community structures of the endophytes were analyzed based on their diversity, colonization, dominance index, and relative frequency of occurrence of the isolated endophytic fungi. A total of 78 isolates have been obtained from three individual plants and clustered into 22 morphotypes consisted of 18 morphotypes of Ascomycota and 4 morphotypes of Basidiomycota divisions. The stolons harbored more endophytes (22.9 %) followed by leaf (16.7 %), root (11.8 %), and petiole (7.6 %). The diversity index was classified as medium category with the highest result (1.91) was found in the root, followed by leaf (1.79), stolon (1.75), and petiole (1.29). The most dominant endophytes were identified as <em>Ceratobasidium </em>sp<em>.</em>, <em>Colletotrichum </em>sp, and <em>Fusarium </em>sp. <em>Ceratobasidium</em> sp. has the highest dominance index (0.02). UPGMA cluster analysis grouped the endophytic fungi into distinct clusters based on the plant parts origin. This study implied that stolon was the the most suitable part of Asiatic pennywort for isolating endophytic fungi. Further study is required to examine the role of the endophytic fungi to produce secondary metabolites in Asiatic pennywort.</p>
Analysis of the role of TpUB05 antigen from Theileria parva in immune responses to malaria in humans compared to its homologue in Plasmodium falciparum; UB05 antigen
Authors: Dinga Jerome Nyhalah , Gamua Stanley Dobgima , Perimbie Stephanie Numenyi , Chuma Francis N. G. , Njimoh Dieudonné Lemuh , Djikeng Apollinaire , Pelle Roger , Titanji Vincent P. K. 
Published: December 2019

Abstract

Background: Despite the amount of resources deployed and technological advancements in Molecular Biology, vaccinology, immunology, genetics, and biotechnology, there is still no effective vaccines against malaria. Immunity to either malaria or East Coast fever is usually seen as species- and/or strain-specific. But there is growing body of evidence suggesting the possibility of the existence of cross strain, cross species and cross genus immune responses in apicomplexans. The principle of gene conservations indicates that homologues play similar role in closely related organisms. UB05 antigen (XP_001347656.2) from P. falciparum is part of chimeric UB05-09 antigen; a potential vaccine candidate has been demonstrated to be a marker of protective immunity in malaria. The homologue of UB05 in T. parva is TpUB05 (XP_763711.1) which was also tested and shown to be a potential marker of protective immunity in ECF as well. In a bid to identify potent markers of protective immunity to aid malaria vaccine development, TpUB05 was tested in malaria caused by P. falciparum .
Results: It was observed that TpUB05 provoked stronger immune responses in malaria compared to UB05 antigen as tested using ELISA, ex-vivo ELISpot assay and in vitro growth inhibition assay.
Conclusion: This study suggests for the first time that TpUB05 from T. parva is a better marker of protective immunity in malaria compared to its homologue UB05 from P. falciparum .
Coxiella burnetii is widespread in ticks (Ixodidae) in the Xinjiang border areas of China
Authors: Ni Jun , Lin Hanliang , Xu Xiaofeng , Ren Qiaoyun , Aizezi Malike , Luo Jin , Luo Yi , Ma Zhan , Chen Ze , Tan Yangchun , Guo Junhui , Liu Wenge , Qu Zhiqiang , Wu Zegong , Wang Jinming , Li Youquan , Guan Guiquan , Luo Jianxun , Yin Hong , Liu Guangyuan 
Published: December 2019

Abstract
Background The gram-negative Coxiella burnetii bacterium is the pathogen that causes Q fever. The bacterium is transmitted to animals via ticks and can cause infection in domestic animals, wild animals, and humans. As the provincial-level administrative region with the largest land area in China, Xinjiang has many endemic tick species; however, the distribution of C. burnetii in ticks in Xinjiang border areas has not been studied in detail.Results For the current study, 1507 ticks were collected from livestock at 22 sampling sites in ten border regions of the Xinjiang Uygur Autonomous region from 2018 to 2019. C. burnetii was detected in 205/348 (58.91%) Dermacentor nuttalli ; in 110/146 (75.34%) Dermacentor pavlovskyi ; in 66/80 (82.50%) Dermacentor silvarum ; in 15/32 (46.90%) Dermacentor niveus ; in 28/132 (21.21%) Hyalomma rufipes ; in 24/25 (96.00%) Hyalomma anatolicum ; in 219/312 (70.19%) Hyalomma asiaticum ; in 252/338 (74.56%) Rhipicephalus sanguineus ; and in 54/92 (58.70%) Haemaphysalis punctata . Among these samples, C. burnetii was detected in D. pavlovskyi for the first time. The infection rate of R. sanguineus was 74.56% (252/338), which was the highest among the four tick genera sampled, whereas the infection rate of H. anatolicum was 96% (24/25), which was the highest among the nine tick species sampled. A sequence analysis indicated that 63 16S rRNA sequences could be found in four newly established genotypes: CXJ-1 (n = 18), CXJ-2 (n = 33), CXJ-3 (n = 6), and CXJ-4 (n = 6).Conclusions This study indicates that CXJ-2 might represent the main C. burnetii genotype in the ticks in Xinjiang because it was detected in eight of the tick species studied. The high infection rate of C. burnetii detected in the ticks found in domestic animals may indicate a high likelihood of Q fever infection in both domestic animals and humans.
Identification and validation of a prognostic index based on a metabolic-genomic landscape analysis of ovarian cancer
Authors: Wang Zi-yao , Li Yukun , Luo Gui-fang , Zou Juan , Chen Chang-ye , Wang Juan , Zeng Xin , Xiao Jiao , Tong Wen-juang , Ma Yan 
Published: December 2019

Abstract
Purpose: Tumor metabolism has been a novel driver of personalized cancer medicine, with aggressive efforts to regulate the metabolic system to prolong their life. The aim of this study is to explore the prognostic value of metabolism in ovarian serous cystadenocarcinoma (OSC), which is the most common subtype of ovarian cancer, accounts for 75-80% of reported cases. Patients and methods: we integrated the expression profiles of metabolism-related genes (MRGs) in survival in 379 OSC patients based on The Cancer Genome Atlas (TCGA) database. Then, several biomedical computational algorithms were employed to identify eight key prognostic MRGs, which were related with overall survival (OS) significantly in OSC. The eight genes represented important clinical significance and prognostic value in OSC. Then a prognostic index was constructed. Results: A total of 701 differentially expressed metabolism-related genes (MRGs) were identified in OSC patients based on TCGA database. Functional enrichment analyses hinted that metabolism may act in a significant role in the development and progression of OSC. Random walking with restart (RWR) algorithm, Univariate cox and lasso regression analysis indicated a prognostic signature based on MRGs (ENPP1, FH, CYP2E1, HPGDS, ADCY9, NDUFA5, ADH1B and PYGB), which performed moderately in prognostic predictions. Conclusion: This study provides a latent prognostic feature for predicting the prognosis of OSC patients and the molecular mechanism of OSC metabolism.
Additional clinical benefits of probiotics as an adjunctive therapy to nonsurgical periodontal treatment of periodontitis: a systematic review and meta-analysis
Authors: Gou Huiqing , Chen Xu , Li Lu , Wang Xiaoqian , Xu Yan 
Published: December 2019

Abstract
Background: With more and more concerns concentrated on the novel therapy applying probiotics, we challenge its trustworthy clinical efficacy as an adjuvant to scaling and root planning (SRP) as compared with SRP alone or combined with placebo or other nonsurgical treatment applied as the initial therapy of periodontitis. Methods: Electronic databases retrieval was performed until October 2017 under certain screening condition. Clinical randomized controlled trials (RCTs) to assess the efficacy of SRP + probiotic versus SRP were included. Primary outcome variables were PPD (pocket probing depth) reduction and CAL (clinical attachment level) gain. Nine publications were eligible for the systematic review and three were evaluated in the meta-analysis. Results: Results demonstrated statistically significant bleeding on probing (BOP) percentage reduction (5.34, p< 0.00001) at short-term, but not a significant differences of overall PPD reduction (0.23mm, p=0.25) for SRP + probiotic treatment versus SRP at short-term, and there was only a tendency (p = 0.08) with regard to overall CAL gain. However significant reduction of PPD and gain of CAL were observed when stratified for deep pockets (0.61mm, p< 0.00001) and for moderate pockets (0.37mm, p=0.006) at short-term respectively. Conclusions: Within the ranges of this study, the adjunctive use of probiotics seem to achieve short-term clinical benefits in the treatment of periodontitis. Conclusions must be treated with caution and future long-term RCTs are needed to testify the clinical application value of probiotics.
Effects of pulsed in-feed antimicrobials on chicken fecal resistome
Authors: Chen Siyuan , Yang Jintao , Zeng Jiaxiong , Hao Jie , Zhao Ruonan , Zeng Zhenling , Xiong Wenguang 
Published: December 2019

Abstract
Background: Antimicrobial resistance has become a global problem that poses great threats to human health. Antimicrobials are widely used in broiler chicken production and consequently affect the fecal resistome. Here we used metagenomic approach to investigate the effects of pulsed antimicrobial administration on chicken fecal resistome. Chickens received three 5-day-course of alone/combined antimicrobials at therapeutic dose, including amoxicillin, chlortetracycline and florfenicol. Chicken feces was collected on T0 (before treatment), T5 (after first treatment), T10 (after second treatment), T15 (after third treatment) at the chicken age of 40 days that are ready for slaughter. Results: Tetracycline resistance gene and polymyxin resistance gene only responded to chlortetracycline and amoxicillin, respectively. The tetracycline resistance gene tetC significantly increased from T0 to T15 in chlortetracycline group. The multidrug resistance gene acrB, acrF, mdtA, mdtK and CRP only occurred significant increase in amoxicillin group (P<0.05). Klebsiella significantly decreased from T0 to T10 in florfenicol group, while significantly increased from T0 to T15 in chlortetracycline group (P<0.05). Amoxicillin did not lead significant change to Klebsiella. Escherichia was always the major host for multidrug resistance genes. ARG-harboring Escherichia increased mainly due to the antimicrobial selection for β-lactam, tetracycline, chloramphenicol resistance genes they harbored. Conclusions: The results indicated that the effects induced by amoxicillin, chlortetracycline and florfenicol significantly shaped the fecal microbiome and resistome. We provided a comprehensive insight into antimicrobial-mediated alteration of chicken fecal microbiome and resistome. These findings will give suggests to veterinarian for combating antimicrobial resistance in broiler chicken production.
The effectiveness of an independent anti-neoplastic medication therapy management system
Authors: Zhang Jianping , Xu Rong , Zhao Xincai , Wang Yonggang , Zhu Wanhu , Xiao Misu , Hu Haiyan , Tang Lina , Guo Cheng , Shen Zan 
Published: December 2019

Abstract
Background: Cancer has been a serious health threat for human especially in developing countries. As the tumor patients are largely in elderly population, the ongoing trend of population aging and increased long-term cancer survivors will add complexity to tumor patients management. However, the tumor patients, aged and with multiple therapeutics, are largely under-served in medication service in China. The aim of this study was to evaluated the effectiveness of an independent anti-neoplastic medication therapy management system for tumor patients management.Methods: An independent, anti-neoplastic medication therapy management (MTM) system, in Shanghai Jiao Tong University affiliated sixth People's Hospital, was established in 2018 as a result of collaboration of onco-chemotherapy, clinical pharmacy and software engineers. The system consists of an independent clinic of pharmacy and MTM software. The software consisted of six modules to enable clinical pharmacists to serve the patients. The six modules are medication therapy review, intervention plan, personal medication record, medication-related action plan, intervention and/or referral, and documentation and follow-up.Results: In a year, 173 tumor patients visited the pharmacy clinic and the averaged clinic visiting count was 2.4 for the all tumor patients. Two thirds patients (117/173) were with one or more identified drug-related problems (DRPs) in medication therapy review. Three leading DRPs are adverse drug reaction, potential drug-drug interaction and non-adherence. Seventy percent of DRPs could be resolved (cure or improved) in four weeks; the medication adherence reached 84%-100% following three or four round of review and intervention.Conclusions: The involvement of clinical pharmacists in polypharmacy tumor patients management increased the communication with oncologists, and could ultimately enhance the effectiveness, safety and rational use of medication.
A post-method condition analysis of using ensemble machine learning for cancer prognosis and diagnosis: a systematic review
Authors: Mirsadeghi Leila , Banaei-Moghaddam Ali Mohammad , Beh-Afarin Seyed Reza , Hosseini Reza Haji , Kavousi Kaveh 
Published: December 2019

Abstract
Background: Ensemble methods are supervised learning approaches that integrate different types of data or multiple individual classifiers. It has been shown that these methods can improve professional performance.Methods: This study is an attempt to provide an in-depth review on 45 most relevant articles and aims to introduce 42 ensemble classifier (EC) machine learning methods used for the detection of 18 different types of cancer. Compared to other types of cancer, breast cancer, and the 22 ensemble methods introduced for its identification, is extensively investigated. The purpose of this study is to identify, map, and analyze the current academic discourse on EC machine learning methods in order to: 1. identify overarching themes emerging from empirical studies as regards EC methods, 2. determine their input data and decision-making strategies, and 3. evaluate relevant statistical procedures.Results: By comparing various approaches, we can introduce Relevance Vector Machine (RVM)-based ensemble learning method that can provide optimal solutions for problems such as curse the dimensionality and high-dimensionality of feature space without missing data values.Conclusions: To obtain robust performance and achieve better results, it is tactfully suggested to use multi-omics data integration, which has demonstrated to identify cancers and their subtypes more efficiently.
Monocentric 8-year clinical follow-up of cladribine-treated patients from registration trials
Authors: Moccia Marcello , Lanzillo Roberta , Petruzzo Martina , Nozzolillo Agostino , Angelis Marcello De , Carotenuto Antonio , Palladino Raffaele , Morra Vincenzo Brescia 
Published: December 2019

Abstract
The authors have requested removal of this preprint.
Hcfc1a regulates neural precursor proliferation and asxl1 expression in the developing brain
Authors: Castro Victoria L. , Reyes Joel F. , Reyes-Nava Nayeli , Paz David , Quintana Anita M 
Published: December 2019

Abstract
Background: Precise regulation of neural precursor cell (NPC) proliferation and differentiation is essential to ensure proper brain development and function. The HCFC1 gene encodes a transcriptional co-factor that regulates cell proliferation, and previous studies suggest that HCFC1 regulates NPC function. However, the molecular mechanism underlying these cellular deficits has not been completely characterized. Methods: Here we created a zebrafish harboring mutations in the hcfc1a gene (the hcfc1aco60/+ allele), one ortholog of HCFC1, and utilized immunohistochemistry and RNA-sequencing technology to understand the function of hcfc1a during neural development.Results: The hcfc1aco60/+ allele results in an increased number of NPCs, neurons, and radial glial cells. These deficits are associated with the abnormal expression of asxl1, a polycomb transcription factor, which we identified as a downstream effector of hcfc1a using high throughput RNA sequencing technology. Inhibition of asxl1 activity and/or expression in larvae harboring the hcfc1aco60/+ allele completely restored the number of NPCs to normal levels. Conclusion: Collectively, our data demonstrate a novel pathway in which hcfc1a regulates NPCs and neurogenesis.
Bjerkandera adusta M1 inhibits Fusarium oxysporum and prevents the wilt incidence in Brassica napus
Authors: feng xiao , Li Yong , Li Suping , Lu Yifan , Zhang Jingjie , Zhu Yunyun , Yang Hongjun , He Xinhua 
Published: December 2019

Abstract
Background: Bjerkandera adusta degrades polycyclic aromatic compounds, such as cellulose and lignin, with the production of laccase and peroxidase. However, its effect on plant disease is unknown. Results: In this study, both the confrontation culture and greenhouse pot experiments were carried out to address the biocontrol mechanisms of B. adusta M1, which was isolated from a unique purple soil (Eutric Regosol), on the growth of pathogenic Fusarium oxysporum f. sp. conglutinans (FOC) and incidence of fusarium wilt in Brassica napus. Results showed that the hyphal growth rate of B. adusta M1 was significantly greater than that of FOC, indicating a strong competitiveness by B. adusta M1. In addition, the B. adusta M1 fermentation broth significantly inhibited the growth of FOC hyphae by 62.79±1.80%, which was greater than an inhibition rate of 40.63±1.68% by the chemical fungicide carbendazim. The image from a scanning electron microscope showed the hyphae of FOC directly was penetrated by the B. adusta M1 hyphae, indicating a strong mycoparasitism by B. adusta M1. Besides, both the B. adusta M1 and B. adusta M1 fermentation broth reduced the incidence and disease index of the fusarium wilt in Brassica napus leaves, and the control effects of different treatments against fusarium wilt were 57.09% and 47.67%, respectively, which were comparable better to 46.11% of the chemical fungicide carbendazim. Furthermore, both the B. adusta M1 and B. adusta M1 fermentation broth increased the activity of superoxide dismutase, catalase, peroxidase and phenylalanine ammonia-lyase, which related to an enhancement of disease resistance. Similarly, both the B. adusta M1 and B. adusta M1 fermentation broth decreased the cell membrane permeability and malondialdehyde contents, thereby reducing the cell membrane damage from the pathogenic fungus. Conclusion: In summary, results from this present study demonstrated that both the Bjerkandera adusta M1 and B. adusta M1 fermentation broth inhibited the growth of FOC and decreased the incidence and disease index of the fusarium wilt disease in Brassica napus. Therefore, B. adusta M1 could be applied as a potential biocontrol fungus to against the fusarium wilt disease.
Cerebral palsy needs a review: a novel DDX3X mutation in a family
Authors: Hu Liqin , Xin Xiaoqin , Lin Shaobin , Luo Min , Chen Junkun , Qiu Hongsheng , Ma Li , Huang Jungao 
Published: December 2019

Abstract
Cerebral palsy (CP) is a non-progressive disorder of movement and posture due to a static insult to the brain. Some conditions of other disorders can mimic CP, and thus CP needs to be pursued for its underlying cause. In this paper, we reported a novel DDX3X mutation in a girl afflicted with MRX102 which had been confused with CP in her early life. The application of whole exome sequencing may be the best method to distinguish between actual CP and other disorders with similar characteristics. A thorough and conscientious review is suggested during the diagnostic progress.