bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ухудшение ситуации с подтоплением, связанное с антропогенным влиянием на примере города Тюкалинска
Authors: Медведков Кирилл Станиславович , Штриплинг Лев Оттович 
Published: October 2019

Актуальность работы обусловлена увеличением влияния антропогенного фактора в развитии процессов подтопления освоенных территорий. В последние годы в Омской области подтоплению подвергается большое количество населенных пунктов, в том числе и местности с благополучными в целом условиями. Одним из таких населенных пунктов является город Тюкалинск, где проблема подтопления серьезно ощущалась в последние четыре года, что и повлияло на выбор объекта исследования. Цель работы: выявление причин обострения ситуации с подтоплением, поиск решений по стабилизации проблемы с учетом влияния реки, осуществляющей естественный дренаж территории. Методы исследования: анализ многолетних наблюдений за подземными водами на скважинах режимной сети мониторинга состояния недр с учетом количества выпавших осадков, выявление причинно-следственных связей с использованием графоаналитических зависимостей. Результаты. Проанализированы многолетние изменения уровня подземных вод, разгрузка которых осуществляется в реку Тюкалка. Изучена интенсивность выпавших осадков на данной территории за периоды обследований. Выявлены причины медленного ухода талых вод весной и накопления подземных вод в многолетний период. Несмотря на достаточно высокую упругость приходно-расходного баланса (атмосферные осадки - поверхностные - подземные воды) и высокую способность к отводу избыточной воды за счет транзитной (пересекающей город) реки Тюкалка, многочисленные антропогенные сооружения, такие как: водопроводящие трубы, плотины, насыпные мосты, дороги и мн. др., вносят существенный вклад в естественный дренаж, понижая расходную часть водного баланса. Регулирование стока реки приводит к ухудшению отвода как поверхностных, так и подземных вод, увеличивая риски развития подтопления и продолжительность весеннего половодья.
Influence of Gas and Oil State on Oil Mobility and Sweet-Spot Distribution in Tight Oil Reservoirs from the Perspective of Capillary Force
Authors: Li Yaohua , Song Yan , Jiang Shu , Wang Qianyou , Jiang Zhenxue , Zhang Fan 
Published: October 2019

Summary
The coexisting free gas, solution gas, and oil in a tight oil reservoir, constrained by the capillary force of the micrometer/nanometer pore/pore-throat system, are of crucial importance to the sweet spot of a tight oil reservoir. Equations of modified total capillary force for the liquid phase are proposed to indicate the tight oil mobility in situ, and are derived from the Kelvin capillary force equation, the Peng-Robinson (PR) equation of state (EOS) (Robinson et al. 1977), and the Van-Laar (VL) equation (Renon and Prausnitz 1968). In addition, relationships between the vapor/liquid state, phase-flow state for production, and gas/oil ratio (GOR) are established in correspondence with the production data of North American and Chinese (Yanchang) tight oil plays. The following conclusions can be drawn.
We proposed a modified total capillary force function to indicate the tight oil mobility in situ, using the PR EOS method and the EOS+λ (VL equation) method. We identified five phase stages according to the gas volume ratio ( yi) in situ: the single-phase oil flow stage, the multiphase transient-flow stage, the multiphase stable-flow stage, the multiphase supercritical-flow stage, and the single-phase gas flow stage. Tight oils are more mobile in three stages: near the critical point of yi-critical(1), such as in the Yanchang and Bakken tight oils; at the transition zone between oil and wet-gas zones before yi-critical(2), such as in the Eagle Ford tight oil; and between the yi-critical(2) and yi-critical(3) in a gas-condensate state, which is at the transition zone between wet-gas and dry-gas zones.
Transcriptome sequencing of a keystone aquatic herbivore yields insights on the temperature-dependent metabolism of essential lipids
Authors: Windisch Heidrun Sigrid , Fink Patrick 
Published: November 2019

Abstract
Background:
Nutritional quality of phytoplankton is a major determinant of the trophic transfer efficiency at the plant-herbivore interface in freshwater food webs. In particular, the phytoplankton’s content of the essential polyunsaturated omega-3 fatty acid eicosapentaenoic acid (EPA) has been repeatedly shown to limit secondary production in the major zooplankton herbivore genus Daphnia. Despite extensive research efforts on the biological model organism Daphnia, and the availability of several Daphnia genomes, little is known regarding the molecular mechanisms underlying the limitations in Daphnia related to dietary EPA availability.
Results:
We used RNA-seq to analyse the transcriptomic response of Daphnia magna which were fed with two different diets — each with or without supplementation of EPA — at two different temperature levels (15 and 20°C). The transcripts were mapped to the D. magna genome assembly version 2.4, containing 26,646 translations. When D. magna fed on green alga, changing the temperature provoked a differential expression of 2,001 transcripts, and in cyanobacteria-fed daphnia, 3,385 transcripts were affected. The supplementation of EPA affected 1,635 (on the green algal diet), or 175 transcripts (on the cyanobacterial diet), respectively. Combined effects for diet and temperature were also observed (669 for the green algal and 128 transcripts for the cyanobacterial diet). Searching for orthologous genes (COG-analysis) yielded a functional overview of the altered transcriptomes. Cross-matched transcript sets from both feed types were compiled to illuminate core responses to the factors temperature and EPA-supplementation.
Conclusions:
Our highly controlled eco-physiological experiments revealed an orchestrated response of genes involved in the transformation and signalling of essential fatty acids, including eicosanoid-signalling pathways with potential immune functions. We provide an overview of downstream-regulated genes, which contribute to enhance growth and reproductive output. We also identified numerous EPA-responsive candidate genes of yet unknown function, which constitute new targets for future studies on the molecular basis of EPA-dependent effects at the freshwater plant-herbivore interface.
Implementing a clinical-educator curriculum to enrich internal medicine residents’ teaching capacity
Authors: Habboush Yacob , Stoner Alexis , Torres Claribel , Beidas Sary 
Published: October 2019

Abstract
Background Physicians-in-training (residents) are typically the primary educators for medical students (MS) during clinical clerkships. However, residents are not formally trained to teach or to assess their teaching. The aim of this study was to assess the implementation of a clinical educator rotation aimed at developing residents’ competencies related to clinical teaching and professional growth. Methods A mixed-methods approach was used to develop and assess the clinical educator rotation at a teaching community hospital. Internal medicine residents who participated in the rotation and consented to the research were assigned to the clinical educator trainee group (CET), the remaining residents were assigned to the control group. Osteopathic MS rotating in the medicine service line were invited to participate. Five key assessment tools were developed and used to measure four constructs: Communication, Professional-Based Learning, Professional Engagement and System-based learning. The study used descriptive and qualitative analyses to measure primary and secondary outcomes. Results The primary outcome measure showed a positive change in resident knowledge, skills and behaviors in communication, reflection, feedback, precepting, and facilitation. MS perceptions of resident teaching skills confirmed the observed changes in CETs. Some CETs continued to practice and build their capacity for teaching after completing the rotation. Qualitatively, we categorized the described codes under four pre-identified themes; communication, professional engagement, professional-based learning, and systemic-based practice. Conclusion Resident teaching and professional capacity is enriched after completing the clinical educator rotation. Other benefits included: enhanced patient communication and education, increased resident confidence, personal satisfaction with training, work life-balance and enhanced career satisfaction. Future research should focus on curricular content, faculty development and delivery assessment. Also, research efforts should identify appropriate emerging technologies to include in the curriculum for enhancing teaching capacity.
Expression analysis of Rab11 during zebrafish embryonic development
Authors: Zhang Haijun , Gao Yu , Qian Peipei , Dong Zhangji , Hao Wenjin , Liu Dong , Duan Xuchu 
Published: November 2019

Abstract
Background: Rab proteins are GTPases responsible for intracellular vesicular trafficking regulation. Rab11 proteins, members of the Rab GTPase family, are known to regulate vesicular recycling during embryonic development. In zebrafish, there are 3 rab11 paralogues, known as rab11a, rab11ba and rab11bb, sharing high identity with each other. However, the expression analysis of rab11 is so far lacking. Results: Here, by phylogeny analysis, we found the three rab11 genes are highly conserved especially for their GTPase domains. We examined the expression patterns of rab11a, rab11ba and rab11bb using RT-PCR and in situ hybridization. We found that all the three genes were highly enriched in the central nervous system, but in different areas of the brain. Apart from brain, rab11a was also expressed in caudal vein, pronephric duct, proctodeum, pharyngeal arches and digestive duct, rab11ba was detected to express in muscle, and rab11bb was expressed in kidney, fin and spinal cord. Different from rab11a and rab11ba, which both have maternal expressions in embryos, rab11bb only expresses during 24hpf to 96hpf. Conclusions: Our results suggest that rab11 genes play important but distinct roles in the development of the nervous system in zebrafish. The findings could provide new evidences for better understanding the functions of rab11 in the development of zebrafish embryos.
Failure patterns after curative resection for intrahepatic cholangiocarcinoma: Possible implications for postoperative radiotherapy
Authors: Yu Wei , Hu Chunxiu , Shui Yongjie , Wu Kui , Zhang Lili , Chen Ying , Li Chao , Xu Jing , Wei Qichun 
Published: October 2019

Abstract
Background: To document the patterns of failure，identify sites at highest risk of recurrence following curative resection of intrahepatic cholangiocarcinoma (IHCC), in order to guide adjuvant postoperative radiotherapy (PORT).

Methods: Patients with IHCC who had undergone radical surgery at our institution from July 2010 to August 2017 were retrospectively analyzed. The survival and prognostic factors were evaluated using univariate and multivariate analysis. All sites of recurrence were recorded and classified including the surgical margin, regional lymph nodes, liver remnant and distant metastasis. The sites at highest risk of recurrence form the basis of the target volume of potential adjuvant radiotherapy.

Results: The median follow-up time was 23.5 months (2-85 months). The median recurrence free survival (RFS) and overall survival (OS) were 12.1 months and 24.8 months, respectively. Seventy-three (73/127, 57.5%) IHCC patients developed tumor recurrence. The number of initial recurrences occurring in the potential PORT volume, remnant liver and distant sits were 46 (46/73, 63.0%), 36 (36/73, 49.3%) and 22 (22/73, 30.1%) cases, respectively. Of the 46 patients whose initial recurrence were inside the potential PORT volume, 29 (29/73, 39.7%) developed recurrence confined to the potential PORT volume, including 13 tumor bed recurrences, 7 lymph node metastases, and 9 with both tumor bed recurrences and lymph node metastases. The most common lymph node metastases were nodes around the abdominal aorta, followed by lymph nodes along the celiac artery, the common hepatic artery, and in the hepatoduodenal ligament.

Conclusions: A high proportion of the recurrences occurred only within the potential PORT volume, implying that adjuvant radiotherapy might improve local-regional control. It seems reasonable to include surgical margins and lymph node stations No.16a2, 9, 8, 12, 13, and 14 in the radiation treatment volume.
Effects of Supervised Early Resistance Training versus standard care on cognitive recovery following cardiac surgery via median sternotomy (The SEcReT study): Protocol for a randomised controlled pilot study
Authors: Pengelly Jacqueline Marie Stacey , Royse Alistair , Bryant Adam , Williams Gavin , Tivendale Lynda , Dettmann Tim , Canty David , Royse Colin , El-Ansary Doa 
Published: November 2019

Abstract
Introduction: Mild cognitive impairment is considered a precursor to dementia and significantly impacts upon quality of life. The prevalence of mild cognitive impairment is higher in the post-surgical cardiac population than the general population, with older age and co-morbidities further increasing the risk of cognitive decline. This significantly impacts upon quality of life. Exercise improves neurogenesis, synaptic plasticity and inflammatory and neurotrophic factor pathways, which may help to augment the effects of cognitive decline. However, the effects of resistance training on cognitive, functional and overall patient-reported recovery have not been investigated in the surgical cardiac population. This study aims to determine the safety and feasibility of early moderate intensity resistance training in people undergoing cardiac surgery via a median sternotomy, compared to standard care. The effect of this exercise program on cognitive and functional recovery will also be examined. Methods: This study will be a prospective, pragmatic, pilot randomised controlled trial comparing a standard care group (low-intensity aerobic exercise) and a moderate-intensity resistance training group. Participants aged 18 years and older with coronary artery and/or valve disease requiring surgical intervention will be recruited pre-operatively and randomised 1:1 to either the resistance training or standard care group post-operatively. Feasibility and safety will be assessed through recruitment and retention rates; exercise program adherence; dropout rate; exercise and session stoppages due to pain, any adverse event or incident; and, any major adverse cardiac and cerebral events. Secondary measures include cognitive function, muscular strength, physical function, multiple-domain recovery, balance and patient satisfaction. Assessments will be conducted at baseline (pre-operatively) and post-operatively at 2 weeks, 8 weeks, 14 weeks and 6 months. Discussion: The results of this study will inform the safety and feasibility of early intervention resistance training for patients following cardiac surgery. In addition, this study will provide insights into the effect of resistance training on postoperative cognitive recovery to inform rehabilitation guidelines. Trial registration: This trial was prospectively registered with the Australian New Zealand Clinical Trials Registry (ANZCTR), ID: ACTRN12617001430325p. Registered on 9 October 2017. Universal Trial Number (UTN):U1111-1203-2131. Keywords: Median sternotomy, resistance training, cognition, cardiac surgery, recovery, rehabilitation, exercise
Global scale transcriptome analysis reveals differentially expressed genes involve in early somatic embryogenesis in Dimocarpus longan Lour.
Authors: Chen Yukun , Xu Xiaoping , Liu Zhuanxia , Zhang Zihao , Xu XuHan , Lin Yuling , lai zhongxiong 
Published: November 2019

Abstract
Background: Somatic embryogenesis (SE) is a process of somatic cells that dedifferentiate to totipotent embryonic stem cells and generate embryos in vitro. Longan SE has been established and wildly used as model system for studying embryogenesis in woody plants, SE-related genes had been characterized. In spite of that, a comprehensive overview of SE at a molecular level is still absent. To understand the molecular mechanisms during longan SE, we examined the transcriptome changes by using Illumina HiSeq from the four distinct developmental stages, including NEC, EC, ICpEC, GE.Results: RNA-seq of the four samples generated a total of 243.78 million high quality reads, approximately 81.5% of the data were mapped to longan genome. The cDNA libraries of NEC, EC, ICpEC and GE, generated 22743, 19745, 21144, 21102 expressed transcripts, 1935, 1710, 1816, 1732 novel transcripts, 2645, 366, 505, 588 unique genes, respectively. Comparative transcriptome analysis showed that a total of 10,642, 4,180, 5,846 and 1,785 genes were differentially expressed in the pairwise comparisons of NEC_vs_EC, EC_vs_ICpEC, EC_vs_GE, ICpEC_vs_GE, respectively. Among them, plant hormones signalling related genes were significantly enriched, especially the auxin and cytokinin signalling components. The transcripts of flavonoid biosynthesis related genes were mainly expressed in NEC, while fatty acid biosynthesis related genes mainly accumulated in early SE. In addition, the extracelluar protein encoding genes LTP, CHI, GLP, AGP, EP1 were related to longan SE. Combined with the FPKM of longan nine tissues transcription, 27 SE specific or preferential genes (LEC1, LEC1-like, PDF1.3, GH3.6, AGL80, PIN1, BBM, WOX9, WOX2, et al.) and 28 NEC preferential genes (LEA5, CNOT3, DC2.15, PR1-1, NsLTP2, DIR1, PIP1, PIP2.1 and POD5 et al.) were characterized as molecular markers for longan early SE. qRT-PCR validation of SE-related genes showed a high correlation between RNA-seq and qRT-PCR data.Conclusion: This study provides new insights into the role of the transcriptome during early SE in longan. Differentially expressed genes reveal that plant hormones signalling, flavonoid and fatty acid biosynthesis, and extracelluar protein related genes were involved in longan early SE. It could serve as a valuable platform resource for further functional studies addressing embryogenesis in woody plants.
The heterogeneous health state profiles of high-risk healthcare utilizers and their longitudinal hospital readmission and mortality patterns
Authors: Ng Shawn Choon Wee , Kwan Yu Heng , Yan Shi , Tan Chuen Seng , Low Lian Leng 
Published: October 2019

Abstract
Background: High-risk patients are most vulnerable during transitions of care. Due to the high burden of resource allocation for such patients, we hypothesize that segmentation of this heterogeneous population into distinct subgroups will enable improved healthcare resource planning. In this study, we segmented a high-risk population with the aim to identify and characterize a patient subgroup with the highest 30-day and 90-day hospital readmission and mortality.
Methods: We extracted data from our transitional care program (TCP) from June to November 2018. Latent class analysis (LCA) was used to determine the optimal number and characteristics of latent subgroups, assessed based on model fit and clinical interpretability. Regression analysis was performed to assess the association of class membership on 30- and 90-day all-cause readmission and mortality.
Results: Among 752 patients, a 3-class best fit model was selected: Class 1 “Frail, cognitively impaired and physically dependent”, Class 2 “Pre-frail, but largely physically independent” and Class 3 “Physically independent”. The 3 classes have distinct demographics, medical and socioeconomic characteristics (p<0.05), 30- and 90-day readmission (p<0.05) and mortality (p<0.01). Class 1 patients have the highest age-adjusted 90-day readmission (OR=2.04, 95%CI: 1.21-3.46, p= 0.008), 30- (OR=6.92, 95%CI: 1.76-27.21, p=0.006) and 90-day mortality (OR=11.51, 95%CI: 4.57-29.02, p<0.001).
Conclusions: We identified a subgroup with the highest readmission and mortality risk amongst high-risk patients. We also found a lack of interventions in our TCP that specifically addresses increased frailty and poor cognition, which are prominent features in this subgroup. These findings will help to inform future program modifications and strengthen existing transitional healthcare structures currently utilized in this patient cohort.
Shu-mu point Catgut embedding therapy for abdominal obesity: study protocol for a randomized controlled trial
Authors: Feng Jialei , Ren Yulan , Jin Yuhao , Meng Jiangqiong , Yang Qiming , Zhong Yuan , Xing Chonghui , Guo Taipin 
Published: October 2019

Abstract
Background: Obesity has become a multifactorial epidemic, affecting individuals, families, societies and countries. Abdominal obesity (AO) is the most harmful kind in obesity. Acupoint catgut embedding (ACE), one of the acupuncture therapies, has been widely used in China while its efficacy has not been tested by randomized controlled trial (RCT). The aim of this study is to evaluate the efficacy and safety of ACE for AO.
Methods: This is a randomized, participant-blind trial. A total of ninety-two patients who met the inclusion criteria for this trial will be enrolled from two independent hospitals and randomly assigned to shu-mu point group and non-acupoint group. Treatment will be conducted once every two weeks, three times for one course. There will be a total of two courses in the whole treatment, and a follow-up will be performed after four weeks of treatment. The primary outcomes are weight and waist circumference (WC). The secondary outcomes are body mass index (BMI), hip circumference (HC), Quality of Life Scale (SF-36), Zung Self-Rating Anxiety Scale (SAS) and Zung Self-Rating Depression Scale (SDS).
Discussion: This trial will evaluate the effectiveness and safety of ACE for AO, and will also observe the changes in the physical conditions of patients before and after the treatment. This trial will be first good quality clinical evidence of ACE for AO.
Association of sleep quality during pregnancy with stress and depression: a prospective birth cohort study in China
Authors: Gao Ming , Hu Jiajin , Yang Liu , Ding Ning , Wei Xiaotong , Li Lin , Liu Lei , Ma Yanan , Wen Deliang 
Published: November 2019

Abstract
Background: The sleep quality of pregnant women in the third trimester is related to mental health. However, there is still a lack of large-scale cohort research exploring this relationship in the second trimester. Thus, we assessed the associations of sleep quality during the second trimester with antenatal stress and antenatal and postnatal depression.
Methods: We examined 1152 pregnant women from a prospective cohort study in China to assess the associations of sleep quality in the second trimester with antenatal stress, antenatal depression, and postnatal depression. We used linear regression models and logistic regression models to examine the associations of sleep quality (Pittsburgh Sleep Quality Index [PSQI]) during pregnancy with perinatal stress (Pregnancy Pressure Scale [PPS]) and depression (Edinburgh Postnatal Depression Scale [EPDS]) status. We further assessed the relationship in groups divided according to maternal age.
Results: PSQI scores were positively associated with antenatal PPS scores (β: 1.52, 95% confidence interval [CI]: 1.28, 1.76), antenatal EPDS scores (β: 0.68, 95% CI: 0.58, 0.78), and postpartum EPDS scores (β: 0.51, 95% CI: 0.38, 0.64). Poor sleep quality (PSQI scores ≥ 5) was associated with antenatal stress status (odds ratio [OR]: 2.60, 95% CI: 1.79, 3.77), antenatal depression status (OR: 3.42, 95% CI: 2.48, 4.72), and postpartum depression status (OR: 2.40, 95% CI: 1.58, 3.64) after adjusting maternal age, BMI, gestational age, smoking, educational level, annual household income and social support. The association of poor sleep quality (PSQI scores ≥ 5) in the second trimester with postnatal depression status was significant among women more than or equal to 30 years old (OR: 4.12, 95% CI: 2.18, 7.78) but not among women less than 30 years old after adjusting covariates above.
Conclusion: Poor sleep quality in the second trimester among Chinese pregnant women is associated with stress and depression symptoms. Strategies to boost sleep quality should be considered during prenatal health care to improve women’s mental health status.