bluuebunny/crossref_metadata_2025_split huggingface dataset:
Turismo de pobreza e infancia. Reflexiones desde la educación para la ciudadanía global
Authors: Millán Escriche Mercedes 
Published: October 2020

<p>La prensa nacional e internacional, así como distintos medios de opinión, muestran numerosas evidencias que resaltan la preocupación por la expansión del turismo de pobreza, sobre todo porque puede resultar lesivo para las poblaciones de determinados destinos turísticos y, particularmente, para la infancia de los mismos. Sin embargo, no resultan muy frecuentes las investigaciones sobre esta temática, salvo en lo referente a las visitas de barrios marginales.</p><p>Esta situación ha llevado a considerar la necesidad de abordar tal problemática en este trabajo, con el objetivo de analizar los rasgos que identifican al turismo de pobreza y de los límites que debería encontrar para su desarrollo, tanto desde la perspectiva legal, que protege los derechos de las personas y especialmente los de los niños, como desde la concepción ética, que debe exigirse a todos los actores implicados en la actividad turística, a partir de los distintos códigos éticos existentes. Asimismo, se buscan estrategias que mejoren esta práctica turística y se llega a concluir que la educación para la ciudadanía global puede aportar determinadas claves que contribuyen a formar en valores y que su implementación en la gestión turística favorecería la configuración de ofertas y productos turísticos cuyos beneficios revertirían en las poblaciones de acogida.</p><p> </p>
Relaxation-induced dipolar exchange with recoupling (RIDER) distortions in CODEX experiments
Authors: Krushelnitsky Alexey , Saalwächter Kay 
Published: October 2020

Abstract. Chemical shift anisotropy (CSA) and dipolar CODEX (Cenralband Only Detection of EXchange) experiments enable abundant quantitative information on the reorientation of the CSA and dipolar tensors to be obtained on millisecond–second timescales. At the same time, proper performance of the experiments and data analysis can often be a challenge since CODEX is prone to some interfering effects that may lead to
incorrect interpretation of the experimental results. One of the most
important such effects is RIDER (relaxation-induced dipolar exchange with recoupling). It appears due to the dipolar interaction of the observed
X nuclei with some other nuclei, which causes an apparent decay in the mixing time dependence of the signal intensity reflecting not molecular
motion, but spin flips of the adjacent nuclei. This may hamper obtaining correct values of the parameters of molecular mobility. In this contribution we consider in detail the reasons why the RIDER distortions remain even
under decoupling conditions and propose measures to eliminate them. That is, we suggest (1) using an additional Z filter between the cross-polarization (CP) section and the CODEX recoupling blocks that suppresses the interfering
anti-phase coherence responsible for the X-H RIDER and (2) recording only the
cosine component of the CODEX signal since it is less prone to the RIDER
distortions in comparison to the sine component. The experiments were
conducted on rigid model substances as well as microcrystalline
2H ∕ 15N-enriched proteins (GB1 and SH3) with a partial
back-exchange of labile protons. Standard CSA and dipolar CODEX experiments
reveal a fast-decaying component in the mixing time dependence of 15N nuclei in proteins, which can be misinterpreted as a slow overall protein
rocking motion. However, the RIDER-free experimental setup provides flat
mixing time dependences, meaning that the studied proteins do not undergo global motions on the millisecond timescale.
Transfer efficacy of Escherichia coli O157:H7 between surfaces of green mature tomatoes and common food processing materials
Authors: Tokarskyy Oleksandr , Korda Mykhaylo , Lukyantseva Halyna 
Published: October 2020

The objectives of this study were: a) to evaluate E. coli O157:H7 survival on green mature tomatoes and squares of common food processing materials – stainless steel, plastic (HDPE), and vinyl conveyor belt (PVC) – post-drying, stored at 25 ºC in the humidified environment for four days; b) to determine pathogen transfer rates (wet, 90 minutes, or 24-hours drying post-inoculation), from inoculated tomato surfaces to uninoculated steel, plastic, and vinyl conveyor belt squares and conversely. It was shown that E. coli O157:H7 did not survive well on the surface of tomatoes, resulting in a decline from 5.3 log10 CFU.mL-1 90 minutes post-drying to 1.4 log10 CFU.mL-1 on day 4. Similarly, the pathogen did not survive well on the surface of food processing squares, with numbers declining over 4 days from 4.04, 4.44, and 4.19 CFU.mL-1 of rinsate 90 minutes squares post-drying to 0.72, 0.50, 0.83 log10 CFU.mL-1, which is close to the detection limit, for the steel, vinyl belt, and plastic, respectively. Successful cross-contamination between tomatoes and food processing surfaces was achieved during wet transfer; while transfer after 90 minutes inoculum post-drying and 24 hours were less successful. This can be explained by both lack of liquid media with suspended bacteria for transfer and fast pathogen die-off after desiccation. Dry transfers, as shown by the percentage of “positive” for pathogen presence tomatoes and squares, as well as bacterial counts, were more successful from tomatoes to squares, but not conversely. Special concern raised vinyl conveyor belt, where the surface picked up the most pathogen cells from the surface of tomatoes, resulting in 100% positive during 90 minute-dry transfers, followed by plastic (66.7% positive) and steel (55.6% positive). To summarize, we presented data on the possibility of cross-contamination between mature green tomatoes and common food processing surfaces, which may be interesting for the processors for risk evaluation.
Conceptismo, epigramatismo y devoción pública en la Sevilla inmaculista: una aproximación al Escuadrón humilde (1616) de Rodrigo Fernández de RiberaConceptism, Epigramatism and Public Devotion in Immaculist Seville: An Approach to Rodrigo Fernández de Ribera’s Escuadrón Humilde (1616)
Authors: Daza Juan Manuel 
Published: October 2020

Abstract
The immaculist movement was one of the most suggestive episodes of the Baroque Seville. Literature, and especially poetry, shows the importance given to the arts in the development of this religious and sociological phenomenon, as can be seen in Rodrigo Fernández de Ribera’s work Escuadrón humilde (humble squad). This work evinces how poetry was not only a festive and apologetic instrument of the faith, but how it could also become a divulgative vehicle with a great doctrinal charge of the ideas that were being debated in literary circles.
El Encomio de los ingenios sevillanos, en la fiesta de los santos Inacio de Loyola y Francisco Javier (1623), de Juan Antonio de Ibarra
Authors: Osuna Inmaculada 
Published: October 2020

Abstract
Encomio de los ingenios sevillanos is a collection of poems from a poetic contest honoring Saint Ignatius of Loyola and Saint Francis Xavier, promoted in Seville by an influential corporation in the commercial field. Juan Antonio de Ibarra’s presentation of the poems in this collection has several meta-literary considerations. The celebration of the contest by the initiative of this civil corporation, several editorial details in the book or its author’s literary links and interests allows us to contextualize the prominence of this meta-literary component and its main purpose: the exaltation of current local poets, continuing with the brilliant trajectory in Seville.
Online Messages Sentiments Analysis Based on Long Short-Term Memory
Authors: Zhao Yunke 
Published: October 2020

In December of 2019, an extremely infectious and deadly pandemic ambushed China. In Wuhan, the novel coronavirus COVID-19 suddenly broke out and spread rapidly to other countries. COVID-19 became a worldwide disaster, affecting not only physical, but also emotional health on a global scale. We wanted to record this change based on the sentiment analysis model and to examine the relationship between world events and the positivity of posts on social media.
To analyze this relationship, we utilized a set of movie reviews as a training sample to construct a sentiment analysis model based on the Long Short-Term Memory neural network theory, and calculate the texts&#39; sentiment score. We then analyzed the overall trend of the data, and discussed the reason behind the tendency. The principal result was that, as the pandemic progressed, online sentiment generally became more positive. We believe that this is because people gradually become more accustomed to life in the COVID-19 era.
Genetic profiling of mistletoe (Viscum album L.) using RAPD-analysis
Authors: Bilonozhko Yu. O. , Rabokon A. N. , PostovoitovA A. S. , Kalafat L. O. , PrivAlikhin S. M. , Pirko N.M. , Demkovych A. Ye. , Blume Ya. B. , Pirko Ya. V. 
Published: October 2020

Aim. The aim of this research was genetic profiling and identification of genetic differences between V. album speciments, growing on deciduous and coniferous species of woody plants using RAPD markers. Methods. The method of polymerase chain reaction (PCR) with random primers (Random Amplification of Polymorphic DNA - RAPD) was used. Amplified DNA fragments were fractionated by electrophoresis in non-denaturing polyacrylamide gel. DNA bands were detected using the staining with silver nitrate. Results. All the studied mistletoe samples were differentiated from each other, and their unique molecular genetic profiles were obtained. 241 amplified DNA fragments were detected in the range from 200 to 2000 bp, 152 fragments (63%) were polymorphic. The samples were divided into two separate groups depending on the type of host plant. Conclusions. The fact that the samples formed two separate clades confirms the assumption that mistletoe, which grow on pine and grow on maple, represents two separate subspecies of V. album.
Keywords: Viscum album L., molecular genetic markers, polymorphism, RAPD.
Optimization of Agrobacterium-mediated transformation of perspective winter wheat genotypes in vitro
Authors: Dubrovna O. V. , Slivka L. V. 
Published: October 2020

Aim. Optimization of conditions for genetic transformation of new perspective winter wheat genotypes. Methods. Agrobacterium-mediated transformation in culture in vitro. Results. The influence of the optical density of cells of the agrobacterial suspension, the concentration of the antibiotic cefotaxime, the duration of coculture on the frequency of obtaining kanamycin-resistant regenerants of new winter wheat genotypes by genetic transformation of callus cultures were investigated using LBA4404 and AGL0 strains. It is shown that depending on the strain the most optimal is the concentration of agrobacteria 0.2-03 OD, duration of coculture for 2-3 days and the use of cefotaxime at a concentration of 250-500 mg/L. Conclusions. The optimal parameters for conducting Agrobacterium-mediated transformation of callus cultures of new perspective winter wheat genotypes were selected.
Keywords: Triticum aestivum, Agrobacterium-mediated transformation, callus cultures.
Genetic and physiological analysis T1 biotechnological plants of winter wheat (Triticum aestivum L.)
Authors: Komisarenko A. G. , Mykhalska S. I. , Kurchii V. M. , Khrystan O. O. 
Published: October 2020

Aim. To investigate inheritance of transgenes in the first generation (T1) of winter wheat biotechnological plants (Triticum aestivum L.). Analyze the performance of T1 genetically modified plants with a double stranded RNA suppressor of the proline dehydrogenase (pdh) gene under normal growing conditions. Methods. PCR analysis, DNA electrophoresis; determination of indicators of the structure of the crop. Results. Molecular genetic analysis was performed and the performance indicators of control and T1 biotechnological plants were investigated. Conclusions. The first generation of genetically modified winter wheat plants resulting from Agrobacterium-mediated transformation in planta confirmed the inheritance of integrated genes. Among the transgenic variants identified plants that lack some fragments of the target gene required for partial suppression of the gene of proline dehydrogenase wheat. It is shown that at the optimal terms of growing biotechnological plants of wheat winter-annual of UK 322/17 and UK 209 h was characterized by the best indexes of structure of harvest as compared to an initial form, while the genetically changed plants of genotypes of UK 95/17 and UK 065 after the elements of the productivity did not differ from control plants.
Keywords: Triticum aestivum L., biotechnological plants, T-DNA, proline dehydrogenase gene, structural analysis indicators.
Tissues of immature and mature embryos as morphogenic competent explants for genetic transformation of wheat
Authors: Mykhalska S. I. , Komisarenko A. G. , Kurchii V. M. 
Published: October 2020

Aim. To analyze the efficiency of using tissues of immature and mature embryos for Agrobacterium-mediated transformation in vitro of new breeding-valuable genotypes of winter wheat (Triticum aestivum L.) for the purpose of their genetic improvement. Methods. Culture of in vitro, extraction and electrophoresis of DNA, PCR analysis. Results. The efficiency of induction of callusogenesis and regeneration of winter wheat shoots was analyzed. The morphogenetic response of callus cultures obtained from different explants under Agrobacterium-mediated transformation in vitro was investigated. Molecular genetic analysis of wheat regenerants for transgenes was performe. Conclusions. The tissues of immature and mature embryos of novel breeding-valuable wheat genotypes are competent explants for Agrobacterium-mediated transformation in vitro. In this case, the tissues of two daily sprouts of mature wheat germ are characterized by higher morphogenetic parameters, which helps to obtain a greater percentage of genetically modified variants.
Keywords: Triticum aestivum L., in vitro, Agrobacterium-mediated transformation, immature embryos, mature embryos.
Combined space-organized system for animals oocytes maturation in vitro
Authors: Syrvatka V. J. , Slyvchuk Yu. I. , Shtapenko O. V. , Gromyko O. M. , Gevkan I. I. 
Published: October 2020

Aim. The aim of the work was to create a space-organized system for oocytes maturation based on rabbits granulosa cells, hyaluronic acid (for structural organization of this system) and insulin, as an important growth factor. Methods. Cultivation of granulosa cells and oocytes maturation in vitro, biochemical determination of lactate dehydrogenase activity and cholesterol concentration, and determination of progesterone and estradiol concentrations by enzyme immunoassay were done. Results. Hyaluronic acid in concentration of 0.5 μg/ml increased proliferation of granulosa cells and progesterone concentration in the conditioned medium on 72 h of cultivation, and estradiol on 48 and 72 hours of cultivation was found. The degree of oocyte maturation to metaphase-2 was 85% by the influence of 10 μg/ml insulin. Same concentration of insulin increased granulosa cells proliferation, estradiol, progesterone and cholesterol concentration in the conditioning medium after 24 hours of cultivation. Conclusions. The optimal parameters of the combined spaceorganized culture system are 1 million cells/ml of granulose with the addition of 0.5 μg/ml hyaluronic acid and 10 μg/ml insulin, which creates the optimal conditions for the maturation of oocytes to metaphase-2 cell cycle.
Keywords: oocytes, granulosa cells, hyaluronic acid, insulin, in vitro.
The application of microbial originating bioregulators to obtain in vitro lines of potato with increased resistance to parasitic nematodes
Authors: Shysha O. M. , Spivak S. I. , Tsygankova V. A. , Iutynska G. O. , Biliavska L. O. , Yemets A. I. , Blume Ya. B. 
Published: October 2020

Aim. Obtaining in vitro new lines of potato (Solanum tuberosum L.) cultivar Vernissage with genetically mediated resistance to the parasitic nematode H. schachtii on nutrient MS media with microbial bioregulators. Methods. In vitro conditions the process of organogenesis of potato plants on nutrient MS media containing microbial bioregulators used at concentrations 25-100 μl/l in combination with phytohormones 2 mg/l BAP and 0,1 mg/l NAA was investigated. Using dot blotting method the degree of hybridization between cytoplasmic si/miRNA isolated from cells of potato plants-regenerants, grown on the artificial invasive background, and nematode mRNA was studied. In the wheat seedlings cell-free system in vitro the silencing activity of si/miRNA isolated from cells of potato plants-regenerants on the template of nematode mRNA was investigated. Results. The experiments conducted in vitro conditions showed that the addition of microbial bioregulators at concentrations 25-100 μl/l in combination with phytohormones 2 mg/l BAP and 0,1 mg/l NAA into MS media increased the efficiency of regeneration of potato shoots to 43–47 % as compared with similar indices obtained on control MS media. The increase of the degree of hybridization to 19-38 % between cytoplasmic mRNA isolated from cells of nematode H. schachtii and si/miRNA isolated from cells of experimental potato plants-regenerants grown in vitro conditions on nutrient media containing bioregulators on the artificial invasive background was shown. Conclusions. Using microbial bioregulators in vitro conditions as components of nutrient MS medium increases potato shoot regeneration efficiency and enhances RNAi-mediated resistance of plants-regenerants to parasitic nematode H. schachtii.
Keywords: microbial bioregulators, potato organogenesis in vitro, potato resistance to nematode Heterodera schachtii, RNA interference.
Alanine scanning of dinitroaniline/phosphorothioamidate site of α-tubulin in plasmodium species distributed in India
Authors: Demchuk O. M. , Karpov P. A. , Rayevsky A. V. , Ozheredov S. P. , Spivak S. I. , Yemets A. I. , Blume Ya. B. 
Published: October 2020

Aim. Identification of amino acid residues participating in specific binding of dinitroaniline and phosphorothioamidate compounds with α-tubulin in Plasmodium falciparum. Methods. Protein structure modelling, protein structure optimization using molecular dynamics method, ligand-protein docking, alanine scanning mutagenesis. Results. Molecular docking of canonical compounds and alanine scanning mutagenesis, indicate two key (Arg2, Val250) and one minor (Glu3) residues involved in binding of both - dinitroaniline and phosphorothioamidate compounds. At the same time, it was revealed two minor residues (Asp251, Glu254) interacting only with some members of dinitroaniline grope. Conclusions. It was identified amino acid residues predetermining existence of joint site and similar interaction of α-tubulin with dinitroani-line and phosphorothioamidate compounds in P. falciparum.
Keywords: malaria, Plasmodium, α-tubulin, molecular interaction, dinitroanilines compounds, phosphorothioamidate compounds, alanine scanning mutagenesis.
Outstanding scientist and bacteriologist Waldemar Haffkine
Authors: Chen I. B. , Humeniuk H. B. 
Published: October 2020

Waldemar Haffkine is an outstanding bacteriologist, immunologist and epidemiologist who was born in Ukraine. He studied at the Department of Natural Sciences at the Imperial Novorossiisk University (now Odessa I.I. Mechnikov National University), and his scientific career as a zoologist began under the guidance of the Nobel Prize for Physiology or Medicine Ilia Mechnikov. Working at the Pasteur Institute, Paris, he developed a vaccine against cholera, tested its effectiveness on himself and for the first time vaccinated people against bacterial diseases. During the cholera epidemic in India, he established a vaccine production, organized preventive vaccinations and inoculated tens of thousands of people, as a result of which morbidity and mortality decreased tenfold. When the plague epidemic struck Bombay, W. Haffkine soon developed a plague vaccine and re-tested its safety. He founded a bacteriological laboratory in Bombay for the production of vaccines and organized large-scale vaccination schemes. The Haffkine Institute still makes millions of doses of vaccines and serums, saving people from cholera, plague, typhus, rabies, tetanus and other diseases.
Keywords: anticholera vaccine, antiplague vaccination, inoculation schemes, the Haffkine Institute.
Brains, brawn, and beauty: The complementary roles of intelligence and physical aggression in attracting sexual partners
Authors: Seffrin Patrick , Ingulli Patricia 
Published: October 2020

AbstractThe current study compared physical aggression to factors affecting socioeconomic status in the accumulation of sex partners over the life course. Our data sample was drawn from the National Longitudinal Study of Adolescent to Adult Health (men, n = 5,636; women, n = 6,787). Participants were examined in terms of the number of lifetime sex partners they reported, nonrelationship partners, cheating or infidelity, and concurrent relationships. Intelligence and physical violence emerged as being especially likely to boost sex partner accumulation for the number of lifetime sex partners and nonrelationship partners in men. Intelligence also interacted positively with men's violence in cross‐sectional models but not longitudinally. Women's violence was not significant regardless of the outcome or model specification. Intelligence showed less consistent effects for women's mating indicators compared to men. Analyses controlled for well‐known correlates of aggression and sexual behavior and factors associated with beauty, including interviewer reports of survey participants' physical attractiveness and maturity, as well as self‐reported attractiveness, maturity, and health. Findings are consistent with evolutionary ideas regarding costly signaling as an effective mating strategy among men.
Highly Efficient Thermally Activated Delayed Fluorescence via an Unconjugated Donor–Acceptor System Realizing EQE of Over 30%
Authors: Peng Chen‐Chen , Yang Sheng‐Yi , Li Hong‐Cheng , Xie Guo‐Hua , Cui Lin‐Song , Zou Sheng‐Nan , Poriel Cyril , Jiang Zuo‐Quan , Liao Liang‐Sheng 
Published: October 2020

AbstractIn this work, two novel thermally activated delayed fluorescence (TADF) emitters, 2tDMG and 3tDMG, are synthesized for high‐efficiency organic light‐emitting diodes (OLEDs), The two emitters have a tilted face‐to‐face alignment of donor (D)/acceptor (A) units presenting intramolecular noncovalent interactions. The two TADF materials are deposited either by an evaporation‐process or by a solution‐process, both of them leading to high OLED performance. 2tDMG used as the emitter in evaporation‐processed OLEDs achieves a high external quantum efficiency (EQE) of 30.8% with a very flat efficiency roll‐off of 7% at 1000 cd m−2. The solution‐processed OLEDs also display an interesting EQE of 16.2%. 3tDMG shows improved solubility and solution processability as compared to 2tDMG, and thus a high EQE of 20.2% in solution‐processed OLEDs is recorded. The corresponding evaporation‐processed OLEDs also reach a reasonably high EQE of 26.3%. Encouragingly, this work provides a novel strategy to address the imperious demands for OLEDs with high EQE and low roll‐off.