bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mitochondrial transplantation for myocardial protection in diabetic hearts
Authors: Doulamis Ilias P , Guariento Alvise , Duignan Thomas , Orfany Arzoo , Kido Takashi , Zurakowski David , del Nido Pedro J , McCully James D 
Published: November 2019

AbstractOBJECTIVESType 2 diabetes causes mitochondrial dysfunction, which increases myocardial susceptibility to ischaemia–reperfusion injury. We investigated the efficacy of transplantation of mitochondria isolated from diabetic or non-diabetic donors in providing cardioprotection from warm global ischaemia and reperfusion in the diabetic rat heart.METHODSEx vivo perfused hearts from Zucker diabetic fatty (ZDF fa/fa) rats (n = 6 per group) were subjected to 30 min of warm global ischaemia and 120 min reperfusion. Immediately prior to reperfusion, vehicle alone (VEH) or vehicle containing mitochondria isolated from either ZDF (MTZDF) or non-diabetic Zucker lean (ZL +/?) (MTZL) skeletal muscle were delivered to the coronary arteries via the aortic cannula.RESULTSFollowing 30-min global ischaemia and 120-min reperfusion, left ventricular developed pressure was significantly increased in MTZDF and MTZL groups compared to VEH group (MTZDF: 92.8 ± 5.2 mmHg vs MTZL: 110.7 ± 2.4 mmHg vs VEH: 44.3 ± 5.9 mmHg; P &lt; 0.01 each); and left ventricular end-diastolic pressure was significantly decreased (MTZDF 12.1 ± 1.3 mmHg vs MTZL 8.6 ± 0.8 mmHg vs VEH: 18.6 ± 1.5 mmHg; P = 0.016 for MTZDF vs VEH and P &lt; 0.01 for MTZL vs VEH). Total tissue ATP content was significantly increased in both MT groups compared to VEH group (MTZDF: 18.9 ± 1.5 mmol/mg protein/mg tissue vs MTZL: 28.1 ± 2.3 mmol/mg protein/mg tissue vs VEH: 13.1 ± 0.5 mmol/mg protein/mg tissue; P = 0.018 for MTZDF vs VEH and P &lt; 0.01 for MTZL vs VEH). Infarct size was significantly decreased in the MT groups (MTZDF: 11.8 ± 0.7% vs MTZL: 9.9 ± 0.5% vs VEH: 52.0 ± 1.4%; P &lt; 0.01 each).CONCLUSIONSMitochondrial transplantation significantly enhances post-ischaemic myocardial functional recovery and significantly decreases myocellular injury in the diabetic heart.
Exploring health system readiness for adopting interventions to address intimate partner violence: a case study from the occupied Palestinian Territory
Authors: Colombini Manuela , Alkaiyat Abdulsalam , Shaheen Amira , Garcia Moreno Claudia , Feder Gene , Bacchus Loraine 
Published: November 2019

Abstract
Domestic violence (DV) against women is a widespread violation of human rights. Adoption of effective interventions to address DV by health systems may fail if there is no readiness among organizations, institutions, providers and communities. There is, however, a research gap in our understanding of health systems’ readiness to respond to DV. This article describes the use of a health system’s readiness assessment to identify system obstacles to enable successful implementation of a primary health-care (PHC) intervention to address DV in the occupied Palestinian Territory (oPT). This article describes a case study where qualitative methods were used, namely 23 interviews with PHC providers and key informants, one stakeholder meeting with 19 stakeholders, two health facility observations and a document review of legal and policy materials on DV in oPT. We present data on seven dimensions of health systems. Our findings highlight the partial readiness of health systems and services to adopt a new DV intervention. Gaps were identified in: governance (no DV legislation), financial resources (no public funding and limited staff and infrastructure) and information systems (no uniform system), co-ordination (disjointed referral network) and to some extent around the values system (tension between patriarchal views on DV and more gender equal norms). Additional service-level barriers included unclear leadership structure at district level, uncertain roles for front-line staff, limited staff protection and the lack of a private space for identification and counselling. Findings also pointed to concrete actions in each system dimension that were important for effective delivery. This is the first study to use an adapted framework to assess health system readiness (HSR) for implementing an intervention to address DV in low- and middle-income countries. More research is needed on HSR to inform effective implementation and scale up of health-care-based DV interventions.
On the Continuity of the Integrated Density of States in the Disorder
Authors: Shamis Mira 
Published: November 2019

Abstract
Recently, Hislop and Marx studied the dependence of the integrated density of states on the underlying probability distribution for a class of discrete random Schrödinger operators and established a quantitative form of continuity in weak* topology. We develop an alternative approach to the problem, based on Ky Fan inequalities, and establish a sharp version of the estimate of Hislop and Marx. We also consider a corresponding problem for continual random Schrödinger operators on $\mathbb{R}^d$.
Matrix Metalloproteinase-13 in Atherosclerotic Plaque Is Increased by Influenza A Virus Infection
Authors: Lee Han Sol , Noh Ji Yun , Shin Ok Sarah , Song Joon Young , Cheong Hee Jin , Kim Woo Joo 
Published: November 2019

Abstract

Background
Influenza virus infection triggers acute cardiovascular events. Several studies have demonstrated that influenza A virus infection was associated with immune cell influx and increased production of inflammatory cytokines in the atherosclerotic plaque lesion, but the underlying mechanism for these findings is not clear.

Methods
We examined the expression levels of matrix metalloproteinases (MMPs) by influenza A virus infection in human cells using quantitative real-time polymerase chain reaction, Western blot, and human MMP-13 enzyme-linked immunosorbent assay. In an animal study, protein expression in the plaque lesions of apolipoprotein E (ApoE)-deficient mice were analyzed by immunohistochemistry and Western blot.

Results
We confirmed that MMP-13 was increased in influenza A virus-infected cells. In the aorta of infected ApoE-deficient mice, MMP-13 was increased at 3 days after infection. Immunohistochemical staining results suggested that collagen was degraded in the MMP-13 expression area and that macrophages were the main source of MMP-13 expression. Furthermore, the expression of MMP-13 was regulated by influenza A virus through activation of the p38 mitogen-activated protein kinase (MAPK) signaling pathway.

Conclusions
In this study, we demonstrated that p38 MAPK-mediated MMP-13 expression by influenza A virus infection led to destabilization of vulnerable atherosclerotic plaques in the artery.
In vitro activity of a novel antibacterial agent, levonadifloxacin, against clinical isolates collected in a prospective, multicentre surveillance study in India during 2016–18
Authors: Appalaraju Boppe , Baveja Sujata , Baliga Shrikala , Shenoy Suchitra , Bhardwaj Renu , Kongre Vaishali , Dattatraya Gogi Suresh , Dhole Tapan , Verma Binita , Mukherjee D N , Gupta Shalini , Shanmugam Priyadarshini , Iravane Jyoti , Mishra Sudhi Ranjan , Barman Purabi , Chopra Shimpi , Hariharan Meenakshi , Surpam Rajendra , Pratap Rana , Joshi Prashant , Khande Hemant , Mane Ashish , Jain Rishi , Bhagwat Sachin 
Published: November 2019

Abstract

Background
Levonadifloxacin is a novel antibiotic belonging to the benzoquinolizine subclass of fluoroquinolones with potent activity against MRSA and quinolone-resistant Staphylococcus aureus. IV levonadifloxacin and its oral prodrug alalevonadifloxacin have recently been approved in India for the treatment of acute bacterial skin and skin structure infections (ABSSSIs) including diabetic foot infections.

Objectives
To investigate the in vitro activity of levonadifloxacin against contemporary clinical isolates collected from multiple tertiary care hospitals across India in the Antimicrobial Susceptibility Profiling of Indian Resistotypes (ASPIRE) surveillance study.

Methods
A total of 1376 clinical isolates, consisting of staphylococci (n = 677), streptococci (n = 178), Enterobacterales (n = 320), Pseudomonas aeruginosa (n = 140) and Acinetobacter baumannii (n = 61), collected (2016–18) from 16 tertiary hospitals located across 12 states in India, were included in the study. The MICs of levonadifloxacin and comparator antibiotics were determined using the reference agar dilution method and broth microdilution method.

Results
Levonadifloxacin exhibited potent activity against MSSA (MIC50/90: 0.5/1 mg/L), MRSA (MIC50/90: 0.5/1 mg/L) and levofloxacin-resistant S. aureus (MIC50/90: 1/1 mg/L) isolates. Similarly, potent activity of levonadifloxacin was also observed against CoNS including MDR isolates (MIC50/90: 1/2 mg/L). Against Streptococcus pneumoniae, levonadifloxacin (MIC50/90: 0.5/0.5 mg/L) showed superior activity compared with levofloxacin (MIC50/90: 1/2 mg/L). Among levofloxacin-susceptible Enterobacterales, 80.6% of isolates were inhibited at ≤2 mg/L levonadifloxacin.

Conclusions
Levonadifloxacin displayed potent activity against contemporary MRSA and fluoroquinolone-resistant staphylococcal isolates, thus offering a valuable IV as well as an oral therapeutic option for the treatment of ABSSSIs. Furthermore, levonadifloxacin exhibited a broad-spectrum activity profile as evident from its activity against streptococci and levofloxacin-susceptible Gram-negative isolates.
Synergistic staphylocidal interaction of benzoic acid derivatives (benzoic acid, 4-hydroxybenzoic acid and β-resorcylic acid) and capric acid: mechanism and verification study using artificial skin
Authors: Kim H W , Seok Y S , Rhee M S 
Published: November 2019

Abstract

Objectives
The present study was designed to investigate a synergistic staphylocidal interaction of antimicrobials.

Methods
The widely used preservative benzoic acid (BzA) and its derivatives [4-hydroxybenzoic acid (HA) and β-resorcylic acid (β-RA)] combined with capric acid (CPA) were investigated.

Results
β-RA was identified as the most effective antimicrobial exhibiting synergistic action with CPA against both Staphylococcus aureus and MRSA. For example, a complete reduction of bacteria (&gt;7.3 log reduction) was obtained within 5 min after treatment with 5.0 mM β-RA (0.079%) plus 0.20 mM CPA (0.004%), while treatment with each material individually showed low bactericidal effects (&lt;1.5 log reduction). Flow cytometry analysis identified membrane disruption related to the synergistic mechanisms, including the following: (i) membrane disruption by CPA (69.2% of cells were damaged by 0.20 mM CPA treatment); (ii) antimicrobial entry through the damaged membrane; and (iii) cytoplasmic ion imbalance resulting in cell death. We verified that the synergistic combination was also effective against MRSA on artificial skin (99.989% elimination after 5 min).

Conclusions
We used only consumer-preferred natural-borne antimicrobials and a very small amount of material was needed based on the synergistic effects. Therefore, these antimicrobials can be widely used as alternative anti-MRSA compounds in healthcare products, cosmetics, pharmaceutical products, foods and for environmental hygiene.
Attractiveness of Pheromone Components With and Without the Synergist, Methyl (2E,4E,6Z)-2,4,6-Decatrienoate, to Brown Marmorated Stink Bug (Hemiptera: Pentatomidae)
Authors: Weber Donald C , Morrison William R , Khrimian Ashot , Rice Kevin B , Short Brent D , Herlihy Megan V , Leskey Tracy C 
Published: November 2019

AbstractThe brown marmorated stink bug, Halyomorpha halys (Stål) (Hemiptera: Pentatomidae) is attracted to its male-produced aggregation pheromone, a ~3.5:1 mixture of (3S,6S,7R,10S)- and (3R,6S,7R,10S)-10,11-epoxy-1-bisabolen-3-ol (SSRS and RSRS respectively), and also to the pheromone of its Asian sympatric species Plautia stali Scott (Hemiptera: Pentatomidae), methyl (2E,4E,6Z)-2,4,6-decatrienoate (MDT). A stereoisomeric mixture of (7R) 10,11-epoxy-1-bisabolen-3-ols (= mixed murgantiols) is used together in commercialized products with MDT because the latter is a synergist for H. halys attraction to mixed murgantiols. However, the optimal ratio for MDT combination with mixed murgantiols, and the sensitivity of bug captures to variation in ratio of the two pheromone components, have not been tested to date. Using black pyramid traps at two sites (in Maryland and West Virginia, United States), different ratios of mixed murgantiols to MDT were tested over two entire seasons. Also, captures using various ratios of the two active pheromone stereoisomers were undertaken in month-long trials with and without MDT. Results showed that H. halys adult and nymphal captures were relatively insensitive to the ratio of synthetic pheromone (mixed murgantiols) to MDT, as long as each was present in the trap. Captures of adults and nymphs were responsive to the lure loading of the SSRS isomer, but relatively insensitive to levels of the minor component, RSRS. The relative insensitivity of H. halys to these attractant ratios gives flexibility to development of more cost-efficient synthesis and trapping as well as other semiochemical-based management tactics.
Lethal Images: Analyzing Extremist Visual Propaganda from ISIS and Beyond
Authors: Baele Stephane J , Boyd Katharine A , Coan Travis G 
Published: November 2019

Abstract
Violent extremist groups regularly use pictures in their propaganda. This practice, however, remains insufficiently understood. Conceptualizing visual images as amplifiers of narratives and emotions, the present article offers an original theoretical framework and measurement method for examining the synchronic and diachronic study of the manipulative use of images by violent extremist groups. We illustrate this framework and method with a systematic analysis of the 2,058 pictures contained in the Islamic State's propaganda magazines targeting Western audiences, exposing the “visual style” of the group, and highlighting the trends and shifts in the evolution of this style following developments on the ground.
DNA barcoding reveals that injected transgenes are predominantly processed by homologous recombination in mouse zygote
Authors: Smirnov Alexander , Fishman Veniamin , Yunusova Anastasia , Korablev Alexey , Serova Irina , Skryabin Boris V , Rozhdestvensky Timofey S , Battulin Nariman 
Published: November 2019

Abstract
Mechanisms that ensure repair of double-strand DNA breaks (DSBs) are instrumental in the integration of foreign DNA into the genome of transgenic organisms. After pronuclear microinjection, exogenous DNA is usually found as a concatemer comprising multiple co-integrated transgene copies. Here, we investigated the contribution of various DSB repair pathways to the concatemer formation. We injected mouse zygotes with a pool of linear DNA molecules carrying unique barcodes at both ends and obtained 10 transgenic embryos with 1–300 transgene copies. Sequencing the barcodes allowed us to assign relative positions to the copies in concatemers and detect recombination events that occurred during integration. Cumulative analysis of approximately 1,000 integrated copies reveals that over 80% of them underwent recombination when their linear ends were processed by synthesis-dependent strand annealing (SDSA) or double-strand break repair (DSBR). We also observed evidence of double Holliday junction (dHJ) formation and crossing over during the concatemer formations. Sequencing indels at the junctions between copies shows that at least 10% of DNA molecules introduced into the zygotes are ligated by non-homologous end joining (NHEJ). Our barcoding approach, verified with Pacific Biosciences Single Molecule Real-Time (SMRT) long-range sequencing, documents high activity of homologous recombination after DNA microinjection.
Atypical organizations and epistatic interactions of CRISPRs and cas clusters in genomes and their mobile genetic elements
Authors: Bernheim Aude , Bikard David , Touchon Marie , Rocha Eduardo P C 
Published: November 2019

Abstract
Prokaryotes use CRISPR–Cas systems for adaptive immunity, but the reasons for the frequent existence of multiple CRISPRs and cas clusters remain poorly understood. Here, we analysed the joint distribution of CRISPR and cas genes in a large set of fully sequenced bacterial genomes and their mobile genetic elements. Our analysis suggests few negative and many positive epistatic interactions between Cas subtypes. The latter often result in complex genetic organizations, where a locus has a single adaptation module and diverse interference mechanisms that might provide more effective immunity. We typed CRISPRs that could not be unambiguously associated with a cas cluster and found that such complex loci tend to have unique type I repeats in multiple CRISPRs. Many chromosomal CRISPRs lack a neighboring Cas system and they often have repeats compatible with the Cas systems encoded in trans. Phages and 25 000 prophages were almost devoid of CRISPR–Cas systems, whereas 3% of plasmids had CRISPR–Cas systems or isolated CRISPRs. The latter were often compatible with the chromosomal cas clusters, suggesting that plasmids can co-opt the latter. These results highlight the importance of interactions between CRISPRs and cas present in multiple copies and in distinct genomic locations in the function and evolution of bacterial immunity.
PELP1 promotes glioblastoma progression by enhancing Wnt/β-catenin signaling
Authors: Sareddy Gangadhara R , Pratap Uday P , Viswanadhapalli Suryavathi , Venkata Prabhakar Pitta , Nair Binoj C , Krishnan Samaya Rajeshwari , Zheng Siyuan , Gilbert Andrea R , Brenner Andrew J , Brann Darrell W , Vadlamudi Ratna K 
Published: November 2019

AbstractBackgroundGlioblastoma (GBM) is a deadly neoplasm of the central nervous system. The molecular mechanisms and players that contribute to GBM development is incompletely understood.MethodsThe expression of PELP1 in different grades of glioma and normal brain tissues was analyzed using immunohistochemistry on a tumor tissue array. PELP1 expression in established and primary GBM cell lines was analyzed by Western blotting. The effect of PELP1 knockdown was studied using cell proliferation, colony formation, migration, and invasion assays. Mechanistic studies were conducted using RNA-seq, RT-qPCR, immunoprecipitation, reporter gene assays, and signaling analysis. Mouse orthotopic models were used for preclinical evaluation of PELP1 knock down.ResultsNuclear receptor coregulator PELP1 is highly expressed in gliomas compared to normal brain tissues, with the highest expression in GBM. PELP1 expression was elevated in established and patient-derived GBM cell lines compared to normal astrocytes. Knockdown of PELP1 resulted in a significant decrease in cell viability, survival, migration, and invasion. Global RNA-sequencing studies demonstrated that PELP1 knockdown significantly reduced the expression of genes involved in the Wnt/β-catenin pathway. Mechanistic studies demonstrated that PELP1 interacts with and functions as a coactivator of β-catenin. Knockdown of PELP1 resulted in a significant increase in survival of mice implanted with U87 and GBM PDX models.ConclusionsPELP1 expression is upregulated in GBM and PELP1 signaling via β-catenin axis contributes to GBM progression. Thus, PELP1 could be a potential target for the development of therapeutic intervention in GBM.
Dynamics and manipulation of ferroelectric domain walls in bismuth ferrite thin films
Authors: Xiao Shuyu , Jin Yaming , Lu Xiaomei , Cheong Sang-Wook , Li Jiangyu , Li Yang , Huang Fengzhen , Zhu Jinsong 
Published: November 2019

Abstract
Ferroelectric domain walls differ from domains not only in their crystalline and discrete symmetry, but also in their electronic, magnetic, and mechanical properties. Although domain walls provide a degree of freedom to regulate the physical properties at the nanoscale, the relatively lower controllability prevents their practical applications in nano-devices. In this work, with the advantages of 3D domain configuration detection based on piezoresponse force microscopy, we find that the mobility of three types of domain walls (tail-to-tail, head-to-tail, head-to-head) in (001) BiFeO3 films varies with the applied electrical field. Under low voltages, head-to-tail domain walls are more mobile than other domain walls, while, under high voltages, tail-to-tail domain walls become rather active and possess relatively long average lengths. This is due to the high nucleation energy and relatively low growth energy for charged domain walls. Finally, we demonstrate the manipulation of domain walls through successive electric writings, resulting in well-aligned conduction paths as designed, paving the way for their application in advanced spintronic, memory and communication nano-devices.
Trillions and Trillions: Herpes Simplex Virus–1 Hepatitis in an Immunocompetent Adult
Authors: Ikuta Kevin , Roychoudhury Pavitra , Xie Hong , Mcclurkan Christopher L , Walkiewicz Magdalena , Makhsous Negar , Huang Meei-Li , Beru Yodit , Alam Mariam , Shepherd Amanda , Lamotte Eric D , Patel Kevin , Morris Amy , Ҫoruh Başak , Yu Lei , Bhattacharya Renuka , Cheng Rex , Walter Roland B , Limaye Ajit P , Lockwood Christina M , Holland Steven M , Rakita Robert M , Koelle David M , Greninger Alexander L 
Published: November 2019

AbstractWe describe a case of acute liver failure and myopericarditis due to herpes simplex virus–1 (HSV-1) in an immunocompetent adult. We estimate that, at the height of viremia, the patient contained a quantity of HSV-1 virions approaching that of human cells. The patient recovered with acyclovir that was dose-adjusted for neurotoxicity and developed a vigorous anti-HSV-1 T-cell response.
Property studies of “loner” flares of gamma-ray blazars
Authors: Wang Gege , Wang Zhongxiang , Chen Liang , Zhou Jianeng , Xing Yi 
Published: November 2019

Abstract
We search through $\gamma$-ray data obtained with the Large Area Telescope (LAT) onboard the Fermi Gamma-Ray Space Telescope and find 24 blazars (or candidates) that have a single clear flare event in their 9.5 yr long-term light curves. We define these events as loner flares since each flare stands out significantly above the relatively stable, low-flux light curve. We analyze the LAT data in detail for these 24 sources. The flares in 10 of them are primarily due to a single sharp peak, for which we study by fitting with two different analytic functions. The time durations thus determined for the sharp peaks are in a range of 4–$25\:$d. The $\gamma$-ray spectra of the 24 blazar sources can be described with a power-law or a log-parabola function. We obtain their spectral properties in the flaring and quiescent states, and find that in the flares 16 of the sources have harder emission, three have softer emission, and the other five keep the same emission. We discuss a possible correlation between the differences in photon index in the quiescent and flaring states and photon indices in quiescence. In addition, the sharp peak flares seem to have a tendency of having long time durations and hard emission, possibly related to their physical origin in a blazar jet. Studies of more similar flares will help establish these possible features.