bluuebunny/crossref_metadata_2025_split huggingface dataset:
Computer- and Telephone-Delivered Interventions on Patient Outcomes and Resource Utilization in Patients With Orthopaedic Conditions
Authors: Jansson Miia Marika , Hyvämäki Piia , Pikkarainen Minna 
Published: September 2020

BACKGROUND:
As the number of patients with orthopaedic conditions has risen continuously, hospital-based healthcare resources have become limited. Delivery of additional services is needed to adapt to this trend.

PURPOSE:
The purpose of this study was to describe the current literature of computer- and telephone-delivered interventions on patient outcomes and resource utilization in patients with orthopaedic conditions.

METHODS:
The systematic review was conducted in January 2019. The standardized checklist for randomized controlled trials was used to assess the quality of the relevant studies. A meta-analysis was not possible due to heterogeneity in the included studies, and a narrative synthesis was conducted to draw informative conclusions relevant to current research, policy, and practice.

RESULTS:
A total of 1,173 articles were retrieved. Six randomized controlled trials met the inclusion criteria, providing evidence from 434 individuals across four countries. Two studies reported findings of computer-delivered interventions and four reported findings of telephone-delivered interventions. The patients who received both computer- and telephone-delivered interventions showed improvements in patient outcomes that were similar or better to those of patients receiving conventional care. This was without any increase in adverse events or costs.

CONCLUSION:
Computer- and telephone-delivered interventions are promising and safe alternatives to conventional care. This review, however, identifies a gap in evidence of high-quality studies exploring the effects of computer- and telephone-delivered interventions on patient outcomes and resource utilization. In future, these interventions should be evaluated from the perspective of intervention content, self-management, and patient empowerment. In addition, they should consider the whole care journey and the development of the newest technological innovations. Additionally, future surgery studies should take into account the personalized needs of special, high-risk patient groups and focus on patient-centric care to reduce postdischarge health problems and resource utilization in this population.
The Effects of Hyperbaric Oxygen on Rheumatoid Arthritis
Authors: Sit Michelle T. , Schmidt Thomas W. , Edmonds Lance D. , Kelly Jason A. , Sky Karen M. , Thornton Jennifer A. , McNeary-Garvin Antoinette M. , Thom Stephen R. , Slade John B. 
Published: September 2020

Background/Objective
This case series pilot study assessed the effects of hyperbaric oxygen therapy (HBO2) for treating rheumatoid arthritis (RA).

Methods
Ten RA subjects received 30 HBO2 treatments over 6 to 10 weeks. Serial rheumatologic evaluations (ie, the Disease Activity Scale [DAS28], the Routine Assessment of Patient Index Data 3, and the Pain and Sleep Quality Questionnaire) were completed at baseline, throughout the course of the study, and at the 6-month follow-up.

Results
There was a statistically significant effect of HBO2 therapy over time on the DAS28–Global Health (p = 0.01), the DAS28–C-reactive protein (p = 0.002), and the DAS28–erythrocyte sedimentation rate (p = 0.008) measures; these analyses excluded 2 patients who were in clinical remission at baseline. Selected post hoc comparisons showed significantly lower DAS28–Global Health, DAS28–C-reactive protein, and DAS28–erythrocyte sedimentation rate scores at 3 and 6 months relative to baseline. In addition, statistically significant decreases in pain as measured by the Routine Assessment of Patient Index Data 3 and Pain and Sleep Quality Questionnaire were observed at the end of HBO2 relative to baseline.

Conclusions
Hyperbaric oxygen therapy is effective for joint pain in patients with RA based on data from multiple, validated clinical measures. Further research with more subjects and the use of a control group is necessary.
Early Detection of Cartilage Degradation Among Heavy Manual Workers Using Serum Level of Collagen Type II Metabolite
Authors: Ramadan Mona , Amr Mahmoud M. , Elsaid Nora Yousry , Fouad Marwa M. 
Published: September 2020

Background
Work-related musculoskeletal disorders are common health problems in brick manufacturers, where mechanical load leads to degenerative joint diseases. Collagen type II metabolite (C2C) is a small peptide excreted in urine, and its serum concentration can directly reflect articular cartilage decomposition.

Objective
Early detection of musculoskeletal disorders among brick workers, using serum C2C as a biomarker of cartilage damage.

Methods
This study involved 88 male brick workers in Arab Abu Saed matched to 88 age- and sex-matched controls. Full history taking, pain assessment using the Standardized Nordic Questionnaire, and complete clinical examination were done for both groups. Serum C2C was measured using a competitive immunoassay method.

Results
Brick workers involved in the study were of a mean age 30.66 ± 7.90 years and mean work duration 14.80 ± 7.89 years, matched to 88 controls. The majority of the participants (77.3%) were of normal body mass index. An increase in pain/discomfort was found among the exposed group. Serum C2C had an increased mean among the exposed group compared with the control. Pearson correlation between serum C2C level, body mass index, age, and years of employment showed no correlation.

Conclusions
Musculoskeletal disorders are prevalent among brick workers who adopt specific awkward postures, unhealthy working conditions, and nonexistent safety procedures, for prolonged periods. Detection of serum C2C level can be used as a predictive biomarker for the early detection of musculoskeletal disorders among brick workers.
Esthetic Repair of Facial Skin Defect After Resection of Malignant Tumor With Lateral Maxillocervical-Island Fasciocutaneous Flap
Authors: Lang Zhongliang , Zhao Liping , Chu Yanjun 
Published: September 2020

Abstract
The face is a high-incidence area for malignant skin tumors, which often need surgically extended resectioning, and the secondary defects generally need esthetic repair. This report introduces a type of flap (lateral maxillocervical-island fasciocutaneous flap)-repair method that can not only repair the defect accurately but also result in skin texture comparable with the surroundings. Moreover, the incision in the flap area is concealed, so as to not cause facial-organ deformity and to achieve an esthetic repair effect. The operation is straightforward and the flap survival rate is high.
An integrative multi-omics approach in Sjögren’s Syndrome identifies novel genetic drivers with regulatory function and disease-specificity
Authors: Teruel María , Barturen Guillermo , Martínez-Bueno Manuel , Barroso Miguel , Castelli Olivia , Povedano Elena , Kerick Martin , Català-Moll Francesc , Makowska Zuzanna , Buttgereit Anne ,   PRECISESADS Clinical Consortium,   PRECISESADS Flow Cytometry Study Group, Pers Jacques-Olivier , Marañón Concepción , Ballestar Esteban , Martin Javier , Carnero-Montoro Elena , Alarcón-Riquelme Marta E. 
Published: September 2020

ABSTRACTPrimary Sjögren’s syndrome (SS) is a systemic autoimmune disease characterized by lymphocytic infiltration and damage of exocrine salivary and lacrimal glands. The etiology of SS is complex with environmental triggers and genetic factors involved. By conducting an integrated multi-omics study we identified vast coordinated hypomethylation and overexpression effects, that also exhibit increased variability, in many already known IFN-regulated genes. We report a novel epigenetic signature characterized by increased DNA methylation levels in a large number of novel genes enriched in pathways such as collagen metabolism and extracellular matrix organization. We identified new genetic variants associated with SS that mediate their risk by altering DNA methylation or gene expression patterns, as well as disease-interacting genetic variants that exhibit regulatory function only in the SS population. Our study sheds new light on the interaction between genetics, DNA methylation, gene expression and SS, and contributes to elucidate the genetic architecture of gene regulation in an autoimmune population.
Comparison of Local Influenza Vaccine Effectiveness Using Two Methods
Authors: Balasubramani GK , Zimmerman Richard K , Eng Heather , Lyons Jason , Clarke Lloyd , Nowalk Mary Patricia 
Published: September 2020

AbstractBackgroundIn some settings, research methods to determine influenza vaccine effectiveness (VE) may not be appropriate because of cost, time constraints, or other factors. Administrative database analysis of viral testing results and vaccination history may be a viable alternative. This study compared VE estimates from outpatient research and administrative databases.MethodsUsing the test-negative, case-control design, data for 2017-2018 and 2018-2019 influenza seasons, were collected using: 1) research methods including consent, specimen collection, RT-PCR testing and vaccine verification using multiple methods; and 2) an administrative database of outpatients with a clinical respiratory viral panel combined with electronic immunization records. Odds ratios for likelihood of influenza infection by vaccination status were calculated using multivariable logistic regression. VE = (1 - OR) × 100.ResultsResearch participants were significantly younger (P<0.001), more often white (69% vs. 59%; P<0.001) than non-white and less frequently enrolled through the emergency department (ED) (35% vs. 72%; P<0.001) than administrative database participants. VE was significant against all influenza and influenza A in each season and both seasons combined (37%-49%). Point estimate differences between methods were evident, with higher VE in the research database, but insignificant due to low sample sizes. When enrollment sites were separately analyzed, there were significant differences in VE estimates for all influenza (66% research vs. 46% administrative P<0.001) and influenza A (67% research vs. 49% administrative; P<0.001) in the ED.The selection of the appropriate method for determining influenza vaccine effectiveness depends on many factors, including sample size, subgroups of interest, etc., suggesting that research estimates may be more generalizable. Other advantages of research databases for VE estimates include lack of clinician-related selection bias for testing and less misclassification of vaccination status. The advantages of the administrative databases are potentially shorter time to VE results and lower cost.
Killip-Kimball Classification Validation, Outcomes and Demographics in an Octogenarian Cohort with Acute Coronary Syndrome in a Developing Country: A Third-Level Multicenter 11-Year Experience
Authors: Ramonfaur Diego , Hinojosa-González David Eugenio , Paredes-Vázquez José G. 
Published: September 2020

AbstractIntroductionThe Killip-Kimball Classification (KC) is used to group patients with acute coronary syndrome (ACS) based on their clinical profile. It has proven to be useful while predicting both short- and long-term mortality. Contemporary data in the elderly population is limited. We sought to analyze trends in outcomes of patients 80 years or older admitted for ACS, by Killip Class. In addition, we assess the validity of the KC in this population.MethodologyA retrospective analysis of patients who underwent a catheterization procedure for ACS was performed. ACS was defined as per AHA guidelines, and included STEMI, non-STEMI and Unstable Angina. We determined factors influencing the KC in which patients present to the emergency room. Likewise, we compared inhospital mortality, length of stay, and other outcomes dividing the patients by KC.ResultsA total of 133 patients were analyzed. Included were: 86, 9, 23 and 15 patients in KC-I through IV respectively with a mean age of 83. The main comorbidities were hypertension (73%), and diabetes (43%). In-hospital mortality was 12%, which was different between KC groups (p < 0.01). In addition, we found higher KC groups to be associated with acute kidney injury during the hospitalization (p< 0.01).ConclusionDespite a strong reduction in mortality for elderly patients with ACS in recent decades, patients presenting with ACS and higher Killip class have a high mortality rate, as described in younger cohorts. The Killip-Kimball classification remains a reliable prognostic tool, with applicability in octogenarian patients.
Limited specificity of commercially available SARS-CoV-2 IgG ELISAs in serum samples of African origin
Authors: Emmerich Petra , Murawski Carolin , Possel Ronald von , Oestereich Lisa , Duraffour Sophie , Pahlmann Meike , Struck Nicole , Eibach Daniel , Krumkamp Ralf , Amuasi John , Maiga-Ascofare Oumou , Rakotozandrindrainy Raphael , Asogun Danny , Ighodalo Yemisi , May Jürgen , Tannich Egbert , Deschermeier Christina 
Published: September 2020

ABSTRACTSpecific serological tests are mandatory for reliable SARS-CoV-2 seroprevalence studies but assay specificity may vary considerably between populations due to interference of immune responses to other pathogens. Here, we assess the false positive rates obtained with four commercially available IgG ELISAs in serum panels originating from three different African countries.Article summary lineSeveral commercially available SARS-CoV-2 ELISAs show limited specificity when applied to serum panels of African origin
Grand battement kinematics and aesthetics in adolescent recreational dancers: Short term effects of static and dynamic hamstrings stretching
Authors: Dierick Frédéric , Buisseret Fabien , Filiputti Loreda , Roussel Nathalie 
Published: September 2020

AbstractWe have studied the kinematics of ballet figures performed by adolescent recreational dancers and determined the most effective muscle stretching modality allowing to increase their physical performance while not harming the aesthetic perception of their motion. Sixteen participants aged between 10 and 19 years were recruited to perform a grand battement, before and after a static or dynamic stretching of hamstring muscles. The three-dimensional kinematics of the grand battement was measured by an optoelectronic system and the aesthetics was scored from a video watched by a jury of professional dancers. Our results show that stretching has a significant impact on grand battement kinematics, and that the most important modifications are induced by dynamic stretching rather than static stretching. Dynamic stretching significantly improves the explosive aspects of the movement (duration and maximal speed), but leads to a significant reduction of its smoothness (jerk). Significant correlations between kinematic parameters and aesthetic scores have been observed, one of them being a positive correlation between thigh’s range of motion and total aesthetic score. These correlations can serve as a reference for movement analysis experts, dancers and their teachers to improve physical performance required by the standards of today’s dance practice without altering the corresponding aesthetic judgment.
Early-life environment programs reproductive strategies through epigenetic regulation ofSRD5A1
Authors: Bar-Sadeh Ben , Eden Or , Pnueli Lilach , Begum Kurshida , Leeman Gregory , Emes Richard D. , Stöger Reinhard , Bentley Gillian R. , Melamed Philippa 
Published: September 2020

AbstractReproductive function and duration of the reproductive life span are phenotypically plastic and programmed in response to the early-life environment. Such adaptive responses are described and rationalized in life history theory in the context of resource availability, but the molecular mechanisms responsible have remained enigmatic. In this study, we hypothesized that epigenetic modifications underlie adaptive reproductive strategies, and found distinct methylation patterns in buccal DNA of Bangladeshi women who grew up in Bangladesh or the UK. The later pubertal onset and lower ovarian reserve associated with Bangladeshi childhood was seen to correlate with more numerous childhood infections, so we adopted a mouse model of pre-pubertal colitis to mimic these conditions. These mice have a similarly-altered reproductive phenotype, which enabled us to determine its mechanistic basis. Several genes encoding proteins with known functions in follicle recruitment were differentially expressed in the mice ovaries, and were also differentially methylated in the women’s buccal DNA. One of these,SRD5A1which encodes the steroidogenic enzyme 5α reductase-1, was down-regulated in the mice ovaries and hyper methylated at the same putative transcriptional enhancer as in the women’s DNA; the levels of methylation correlating with gene expression levels.Srd5a1expression was down-regulated also in the hypothalamus where 5α reductase-1 catalyzes production of neurosteroids that regulate gonadotropin releasing hormone (GnRH). Chemical inhibition of this enzyme affected both GnRH synthesis and release, and resulted in delayed pubertal onsetin vivo. The activity of 5α reductase-1 in hypothalamus and ovary and the sensitivity ofSRD5A1to epigenetic regulation attest to its role in directing long-term physiological strategies in response to environmental conditions. In the reproductive axis, this includes timing of pubertal onset, adult reproductive function and duration of the reproductive lifespan.
A method to remove the influence of fixative concentration on post-mortem T2maps using a Kinetic Tensor model
Authors: Tendler Benjamin C. , Qi Feng , Foxley Sean , Pallebage-Gamarallage Menuka , Menke Ricarda A.L. , Ansorge Olaf , Hurley Samuel A. , Miller Karla L. 
Published: September 2020

AbstractFormalin fixation has been shown to substantially reduce T2estimates when performing post-mortem imaging, primarily driven by the presence of bulk fixative in tissue. Prior to scanning, post-mortem tissue samples are often placed into a fluid that has more favourable imaging properties, such as matched magnetic susceptibility. This study investigates whether there is any evidence for a change in T2in regions close to the tissue surface in post-mortem T2maps due to fixative outflux into this surrounding fluid. Furthermore, we investigate whether a simulated spatial map of fixative concentration can be used as a confound regressor to reduce T2inhomogeneity. To achieve this, T2maps and diffusion tensor estimates were obtained in 14 whole, formalin fixed post-mortem brains placed in fluorinert approximately 48 hours prior to scanning. This consisted of 7 brains fixed with 10% formalin and 7 brains fixed with 10% neutral buffered formalin (NBF). Fixative outflux was modelled using a Kinetic Tensor (KT) model, which incorporates voxelwise diffusion tensor estimates to account for diffusion anisotropy and tissue-specific diffusion coefficients. Brains fixed with 10% NBF revealed a spatial T2pattern consistent with the modelled fixative outflux. Confound regression of fixative concentration reduced T2inhomogeneity across both white and grey matter, with the greatest reduction attributed to the KT model vs simpler models of fixative outflux. No such effect was observed in brains fixed with 10% formalin. Correlations with ferritin and myelin proteolipid protein (PLP) histology lead to an increased similarity for the relationship between T2and PLP for the two fixative types after KT correction. Only small correlations were identified between T2and ferritin before and after KT correction.
Transcriptomic analysis of naïve human embryonic stem cells cultured in three-dimensional PEG scaffolds
Authors: McKee Christina , Brown Christina , Bakshi Shreeya , Walker Keegan , Govind Chhabi K. , Chaudhry G. Rasul 
Published: September 2020

AbstractDerivation of primed and naïve human embryonic stem cells (ESCs) have prompted an increased interest in devising culture conditions for maintaining their pluripotency and differential potential. Naïve ESCs are characterized by improved viability, proliferation, and differentiation capacity in comparison to primed ESCs. However, traditional two-dimensional (2-D) cell culture techniques fail to mimic the three-dimensional (3-D)in vivomicroenvironment, which results in altered morphological and molecular characteristics of ESCs. Here, we describe the use of 3-D self-assembling scaffolds that support growth and maintenance of the naïve state characteristics of human ESC line, Elf1. Scaffolds were formed via a Michael addition reaction upon combination of two 8-arm polyethylene glycol (PEG) polymers functionalized with thiol (PEG-8-SH) and acrylate (PEG-8-Acr) end groups. 3-D scaffolds not only maintained the naïve state, but also supported long-term growth for up to 3 weeks without requiring routine passaging and manipulation. 3-D grown cells exhibited upregulation of core (OCT4,NANOG, andSOX2) and naïve (KLF17,KLF4,TFCP2L1,DPPA3, andDNMT3L) genes. These genes returned to normal levels when 3-D grown cells were propagated under 2-D culture conditions. Examination of RNA-sequencing demonstrated significant changes in gene expression profiles between 2-D and 3-D grown Elf1 cells. Gene Ontology analysis revealed upregulation of biological processes involved in the regulation of transcription and translation, as well as β-catenin-TCF complex assembly, extracellular matrix organization, and chromatin remodeling in 3-D grown Elf1 cells. 3-D culture conditions also induced upregulation of genes associated with several signaling pathways including Wnt signaling and focal adhesion. However, p53 signaling pathway associated genes were downregulated under these culture conditions. Our findings provide insight into the possible mechanisms of prolonged self-renewal as well as upregulation of pluripotent genes stimulated by the transduction of mechanical signals from the 3-D microenvironment.
Tuning of self-renewing capacity in theArabidopsisstomatal lineage by hormonal and nutrition regulation of asymmetric cell divisions
Authors: Gong Yan , Alassimone Julien , Varnau Rachel , Sharma Nidhi , Cheung Lily S. , Bergmann Dominique C. 
Published: September 2020

ABSTRACTAsymmetric and self-renewing divisions build and pattern tissues. In theArabidopsis thalianastomatal lineage, asymmetric cell divisions, guided by polarly localized cortical proteins, generate the majority of cells on the leaf surface. These divisions can be fine-tuned by systemic and environmental signals to modify tissue development, but the molecular mechanisms by which plants incorporate such cues to regulate asymmetric divisions are largely unknown. In a screen for modulators of cell polarity and asymmetric divisions, we identified a mutation inCONSTITIUTIVE TRIPLE RESPONSE 1, a negative regulator of ethylene signaling. We subsequently revealed antagonistic impacts of ethylene and glucose signaling on the self-renewing capacity of stomatal lineage stem cells. Quantitative analysis of the impacts of these signaling systems on cell polarity and fate dynamics showed that developmental information may be encoded in both the spatial and temporal asymmetries of polarity proteins. Taken together, our results provide a framework for a mechanistic understanding of how systemic information such as nutritional status and environmental factors tune stem cell behavior in the stomatal lineage, ultimately enabling optimization of leaf size and cell-type composition.