bluuebunny/crossref_metadata_2025_split huggingface dataset:
Splicing Regulator p54nrb/Non–POU Domain–Containing Octamer‐Binding Protein Enhances Carcinogenesis Through Oncogenic Isoform Switch of MYC Box–Dependent Interacting Protein 1 in Hepatocellular Carcinoma
Authors: Hu Zhixiang , Dong Liangqing , Li Shengli , Li Zhe , Qiao Yejun , Li Yuchen , Ding Jie , Chen Zhiao , Wu Yangjun , Wang Zhen , Huang Shenglin , Gao Qiang , Zhao Yingjun , He Xianghuo 
Published: December 2019

Background and Aims
Alternative splicing (AS) is a key step that increases the diversity and complexity of the cancer transcriptome. Recent evidence has highlighted that AS has an increasingly crucial role in cancer. Nonetheless, the mechanisms underlying AS and its dysregulation in hepatocellular carcinoma (HCC) remain elusive. Here, we report that the expression of RNA‐binding protein p54nrb/non‐POU domain‐containing octamer‐binding protein (NONO) is frequently increased in patients with HCC and is associated with poor outcomes.

Approach and Results
Knockdown of NONO significantly abolished liver cancer cell proliferation, migration, and tumor formation. RNA‐sequencing revealed that NONO regulates MYC box–dependent interacting protein 1 (or bridging integrator 1 [BIN1]; also known as amphiphysin 2 3P9) exon 12a splicing. In the normal liver, BIN1 generates a short isoform (BIN1‐S) that acts as a tumor suppressor by inhibiting the binding of c‐Myc to target gene promoters. In HCC, NONO is highly up‐regulated and produces a long isoform (BIN1‐L, which contains exon 12a) instead of BIN1‐S. High levels of BIN1‐L promote carcinogenesis by binding with the protein polo‐like kinase 1 to enhance its stability through the prevention of ubiquitin/proteasome‐dependent cullin 3 degradation. Further analysis revealed that NONO promotes BIN1 exon 12a inclusion through interaction with DExH‐box helicase 9 (DHX9) and splicing factor proline and glutamine–rich (SFPQ). Notably, frequent coexpression of DHX9–NONO–SFPQ is observed in patients with HCC.

Conclusions
Taken together, our findings identify the DHX9–NONO–SFPQ complex as a key regulator manipulating the oncogenic splicing switch of BIN1 and as a candidate therapeutic target in liver cancer.
USING ROTATING SPRINKLER GUNS IN CENTRE‐PIVOT IRRIGATION SYSTEMS†
Authors: Baiamonte Giorgio , Baiamonte Gabriele 
Published: December 2019

AbstractThe use of centre‐pivot irrigation systems has significantly increased in the last few decades, since they are easier and less time‐consuming to manage compared to other irrigation systems. Standard high‐pressure impact sprinklers, low‐pressure spray sprinklers or LEPA (low energy precision application) systems are usually combined with a centre‐pivot irrigation system (CP‐SYS). However, when applying waste water or with reclaimed effluents that are extremely dirty, small nozzle sizes that require CP‐SYS could become plugged. As an alternative to common centre‐pivot irrigation systems, this paper analyses the ‘geometry’ of areas irrigated by rotating sprinkler guns in a centre‐pivot irrigation system (CP‐RoSYS), which provide a solution to the plugging phenomena, because they have larger‐sized nozzles. Overlapping irrigated areas along the tangential and radial lateral directions, i.e. for multiple sprinkler guns, are studied. According to two parameters defining the geometry of the centre‐pivot system, simple relationships of the radius of throw along the laterals are derived. The hydraulics and efficiency analysis of the centre‐pivot system, coupled with rotating sprinkler guns, were not examined in this study, but the results could be useful for studying the water distribution uniformity, hydraulic behaviour and efficiency of CP‐RoSYS compared with other types of irrigation systems. © 2019 John Wiley & Sons, Ltd.
Diffuse reflectance spectroscopy as a monitoring tool for gastric mucosal devitalization treatments with argon plasma coagulation
Authors: Osorio Juan D. , Vilches Sergio , Zappe Hans 
Published: December 2019

AbstractElectrosurgery with argon plasma coagulation is a widespread technique used in various medical fields for applications which range from hemostasis to devitalization processes. Developing tools which provide feedback concerning tissue condition during these surgeries is fundamental for improving the safety and success of this treatment. We present here a method based on diffuse reflectance spectroscopy to monitor gastric mucosal devitalization treatments. The analysis of the diffusely reflected spectra of the tissue allows us to differentiate between ablation states by using linear discriminant analysis (LDA) as a classification algorithm. An ex vivo pilot study on several swine stomachs showed promising results, with 97.8% of correctly classified ablation states on a new unseen stomach, encouraging further tests with human tissue.
Opening up while locked down: Client disclosure in correctional settings
Authors: Ort Daisy 
Published: December 2019

AbstractIncarcerated individuals experience mental health symptoms resulting from the stress of incarceration, as well as from prior histories of trauma. In addition, the growth of the prison population and the increase in sentence lengths have eroded conditions in correctional facilities, leading to increased stress, trauma, and other mental health issues. Psychologists can help incarcerated individuals address existing mental health issues, cope with the trauma of incarceration, and prepare for successful re‐entry into society. However, the effectiveness of psychotherapy may be hampered by a number of factors that inhibit client disclosure in correctional settings. Based on interviews with forensic psychologists and an incarcerated patient, this paper explores the circumstances that prevent disclosure in correctional settings and proposes recommendations for psychologists to facilitate disclosure to improve therapeutic effectiveness.
Understanding Misdemeanor Enforcement: The Roles of Calls for Service and Community Characteristics
Authors: Glazener Emily M. , Kozlowski Meghan M. , Lynch James P. , Smith Jinney S. 
Published: December 2019

AbstractThis study examines the roles of calls for service (i.e., police‐related 911 calls) and community characteristics in explaining variation in enforcement rates for low‐level, misdemeanor offenses, which make up the large majority of police enforcement activity. The study site is Prince George's County, Maryland, and the unit of analysis is the police department's 65 patrol beats, studied over a 10‐year period, during 2006–2015. Overall, misdemeanor enforcement rates vary at the beat level, and that variation can be largely explained using a combination of indicators about community characteristics and calls for service. The findings indicate, though, that the calls for service rate is the most important variable in explaining misdemeanor enforcement variation. These findings inform both future research on police activity, and current policy debates about what drives enforcement rates and the role of discretion in enforcement outcomes.
Influence of Different Additives on the Interaction of Quinolone Antibiotic Drug with Surfactant: Conductivity and Cloud Point Measurement Study
Authors: Rahim Md. Abdur , Mahbub Shamim , Islam Md. Kamrul , Ahsan Sk. Md. Ali , Rana Shahed , Rub Malik Abdul , Khan Anish , Hoque Md. Anamul 
Published: December 2019

AbstractConductometric and cloud point (CP) measurement studies have been performed to investigate the interaction of tetradecyltrimethylammonium bromide (TTAB) and Triton® X‐100 (TX‐100) with ciprofloxacin hydrochloride (CFH) in different solvents over the temperature range of 295.15–315.15 K. CFH is used for the treatment of various bacterial infections. The observed critical micelle concentration (CMC) values of TTAB were found to be reduced in the presence of electrolytes (Na2SO4/Na3PO4), and this reduction proceeds with the elevation of salt concentration. The order of the CMC of TTAB follows the trend:  >  >. The observed CMC values of TTAB were found to increase with increasing temperature and decrease with increasing concentration of CFH in aqueous medium. The values of Gibbs free energy of micellization () for the TTAB/TTAB + CFH mixture were found to be negative, implying spontaneous micellization. The estimated CP of TX‐100 decreases with increasing concentration of TX‐100 in aqueous medium. The CP values first decrease with increasing concentration of CFH and then increase at higher concentration of CFH almost in all cases investigated. The values of free energy of clouding were found to be positive in all cases studied implying that phase separation of TX‐100 was nonspontaneous. The other thermodynamic parameters associated with the micellization of TTAB and the phase separation of TX‐100 were estimated and explained.
Kazakhstan Gulag heritage: Dark tourism and selective interpretation
Authors: Lennon Justin John , Tiberghien Guillaume 
Published: December 2019

AbstractKazakhstan holds some of the most significant Gulag heritage sites; however, tourism research remains limited. This article introduces analysis of contrasting sites and considers how some have been developed and others ignored. Selectivity in interpretation is linked to societal amnesia and the collective trauma experienced by the population of Kazakhstan. The article reaffirms the politicization of heritage in this emergent nation.
Lidocaine promotes fibroblast proliferation after thermal injury via up‐regulating the expression of miR‐663 and miR‐486
Authors: Wu Sheng‐Gang , Li Hua‐Tao , Wang Li‐Li , Yan Lei 
Published: December 2019

AbstractThermal burn is a complex injury that induces pronounced inflammatory reactions and destruction of the microvasculature. Lidocaine, which is broadly used for pain relief, has been reported have the capacity to modulate wound healing processes. Seventeen burn injury patients with no treatment and nine controls were included in this study. The expression of 15 candidate miRNAs in the dermis samples were detected by qRT‐PCR. The target genes were predicted using online bioinformatics tools and confirmed by dual luciferase assay and immunoblotting. The function of miR‐486 and miR‐663 on skin fibroblasts keratinocytes were determined by MTT assay and flow cytometry. The level of miR‐486 and miR‐663 was increased after burn injury, meanwhile, the highest level of miR‐486 and miR‐663 was found after lidocaine treatment. We identified that BCL2L14 was a direct target of both miR‐486 and miR‐663. Meanwhile, overexpression of miR‐486 or miR‐663 inhibit apoptosis and promoted proliferation of human skin fibroblasts keratinocytes. These results indicated that lidocaine treatment promoted the skin healing after thermal injury through up‐regulating miR‐486 and miR‐663 expression, and partially explained how lidocaine modulates wound healing processes. This may provide an evidence for the therapeutic effect of lidocaine during skin healing process. However, the underlying mechanisms need to be further unveiled.
Binder and Interphase Microstructure in a Composite Material Characterized by Scanning Electron Microscopy and NMR Spin Diffusion Experiments
Authors: Parisse Silène , Petit Jean‐Fabien , Forzy Alexandre , Lecardeur Alexandre , Beaugrand Sandrine , Palmas Pascal 
Published: December 2019

Abstract1H NMR spin diffusion is shown to advantageously complement scanning electron microscopy (SEM) observations for the characterization of polymer structuring in composite materials. It is here demonstrated on a material containing a few percent of polymer binder and a crystalline organic/inorganic mixture as reinforcement. In SEM observations, polymer accumulations are seen. However, the polymer is also expected to fill small porosities and thin layers at the interface of particles to ensure the cohesion and the mechanical properties of the assembly. In most cases, this polymer structuring is invisible considering the resolution and contrast achieved by SEM on such material. It is thus investigated taking advantage of the two‐step decay of nuclear magnetic resonance spin diffusion curves. Average thickness values of 30 and 188 nm are estimated for the interphase and the overall polymer, respectively. Such structural information improves the knowledge of structure/property relationships and provides better understanding of material properties and making processes.
RETRACTED: Genetic polymorphism and phylogenetic analyses of 21 non‐CODIS STR loci in a Chinese Han population from Shanghai
Authors: Zhou Zhihan , Shao Chengchen , Xie Jianhui , Xu Hongmei , Liu Yidong , Zhou Yueqin , Liu Zhiping , Zhao Ziqin , Tang Qiqun , Sun Kuan 
Published: December 2019

AbstractBackgroundShort tandem repeats (STRs) are essential genetic markers for forensic applications and population estimations; thus the population genetics of STR loci have been extensively studied and discussed.MethodsIn the present study, we detected 21 autosomal noncombined DNA index system (non‐CODIS) STR loci in a Chinese Han population from Shanghai, calculated their forensic parameters and analyzed their genetic relationships with reported reference populations in mainland China.ResultsA total of 173 alleles were observed, with corresponding allele frequencies from 0.0020 to 0.5512. The cumulative power of discrimination (CPD) and the cumulative probability of exclusion (CPE) values of the 21 STR loci were 0.999999999999999999997337058271 and 0.99999953732495, respectively. The results of interpopulation differentiation, phylogenetic, multidimensional scaling, and structure analyses indicated a closer genetic relationship of the studied population with Han populations from other regions of China than with other populations.ConclusionsThe 21 STR loci exhibited high genetic polymorphism in the studied Shanghai_Han population and could be used for forensic applications and population genetic studies.
A systematic review of the effectiveness of promoting water intake to reduce sugar‐sweetened beverage consumption
Authors: Dibay Moghadam Sepideh , Krieger James W. , Louden Diana K. N. 
Published: December 2019

SummaryObjectiveTo examine whether the promotion of water intake could reduce sugar‐sweetened beverage (SSB) consumption or purchases independent of interventions that target SSBs.MethodsSeven databases were systematically searched. Included studies used water promotion as the primary intervention; used a controlled trial, single group pre‐post, or prospective cohort study design; included a measure of SSB consumption or purchase; enrolled human participants of any age who lived in high‐income or middle‐income countries; contained original data; and appeared in a peer‐reviewed English‐language article published from 1 January 2000 to January 4, 2019. The search yielded 7068 publications, from which 108 were chosen for full‐text review. Seventeen were included in this review.ResultsNine of the 17 studies were randomized controlled trials, six were nonrandomized controlled trials, and 2 were single‐group pre‐post studies. Participants were primarily children and adolescents. Interventions included water provision, education or promotion activities. Ten of 17 studies were at low or some/moderate risk of bias. Seven studies showed a statistically significant decrease in SSB consumption of which only 2 were at low or some/moderate risk of bias.ConclusionsThis review found limited evidence that interventions aimed solely at increasing water consumption reduce SSB intake. Further research is needed to investigate whether interventions that combine water promotion and SSB reduction strategies could be synergistic for reducing SSB intake.
Preconception leptin levels and pregnancy outcomes: A prospective cohort study
Authors: Plowden Torie C. , Zarek Shvetha M. , Rafique Saima , Sjaarda Lindsey A. , Schisterman Enrique F. , Silver Robert M. , Yeung Edwina H. , Radin Rose , Hinkle Stefanie N. , Galai Noya , Mumford Sunni L. 
Published: December 2019

SummaryObjectiveObesity has become a major, worldwide public health issue and is associated with a greater risk of adverse pregnancy outcomes. Leptin, a hormone produced by adipocytes, is elevated in individuals with obesity and may mediate the association between obesity and pregnancy outcomes. Though leptin levels during pregnancy have been associated with pregnancy outcomes, less is understood regarding preconception levels. Therefore, the objective of this study was to evaluate associations between preconception leptin levels and adverse pregnancy outcomes.MethodsThis was a prospective cohort study nested within a large randomized controlled trial conducted at four medical centres in the United States. A total of 1078 women completed the parent study; this analysis involved women who became pregnant during that study (n = 776). Patients were healthy women, ages 18 to 40, attempting to conceive, with 1 to 2 prior pregnancy losses. Participants were followed for less than or equal to 6 cycles while trying to conceive and throughout pregnancy if they conceived. Preconception leptin concentrations were measured in serum collected at baseline then categorized by tertiles (using the lowest as reference group). Weighted log‐binomial regression estimated risk ratios (RR) and 95% confidence intervals (CIs) for pregnancy loss, preterm delivery (PTD), gestational diabetes (GDM), and hypertensive disorders in pregnancy, adjusting for age, waist‐to‐hip ratio (WHR), and body mass index (BMI).ResultsThe mean (SD) BMI in this cohort was 25.4 ± 6.0. GDM (RR 18.37; 95% CI, 2.39‐141.55) and hypertensive disorders of pregnancy (RR 2.35; 95% CI, 1.20‐4.61) risks were higher among women in the high tertile after adjusting for age and WHR. The associated risk persisted when adjusting for BMI for GDM but was attenuated for hypertensive disorders in pregnancy. Leptin levels were not associated with risk of pregnancy loss or PTD.ConclusionsWomen with higher baseline preconception leptin levels had a higher likelihood of experiencing some adverse pregnancy outcomes including GDM and hypertensive disorders of pregnancy. These findings warrant further evaluation, especially in light of the association between leptin and obesity.
The preparation and characterization of buckypaper made from carbon nanotubes impregnated with chitosan
Authors: Alosime Eid M. , Alshahrani Ahmed A. , Nghiem Long D. , in het Panhuis Marc 
Published: December 2019

AbstractBiopolymer chitosan was incorporated into a thin multiwalled carbon nanotube membrane (MWNT buckypaper) via filtration and soaking in 0.1% (w/v) of low‐molecular‐weight (MW) chitosan. The properties of the buckypaper membrane before and after annealing and after soaking were characterized by measurement of their electrical conductivities (19 ± 2 to 42 ± 2 S/cm), contact angles (31 ± 4° to 71 ± 4°), and mechanical properties (tensile strength, small‐ranging between 1.4 ± 0.1 and 4.2 ± 0.7 MPa; Young's modulus: 85 ± 4 to 443 ± 20 MPa). Moreover, the morphological properties, surface area, and permeability toward water of these buckypaper membranes were characterized and compared with corresponding carbon nanotube membranes prepared with Triton X‐100 (Trix) as the surfactant. Scanning electron microscopic (SEM) images and Brunauer, Emmett, and Teller (BET) data of MWNT‐annealing buckypaper membranes revealed that the diameters of their surface pores were significantly higher than that of the corresponding buckypaper membranes soaked in chitosan solution. The solution of chitosan incorporated inside the porous structure of the annealed MWNT membrane led to a significantly reduced surface area and pore size distribution of the composite membrane, revealing that this could be a useful method for desalination.
Effect of chemical treatment on mechanical properties and water diffusion characteristics of jute‐polypropylene composites
Authors: Chandekar Harichandra , Chaudhari Vikas , Waigaonkar Sachin , Mascarenhas Adlete 
Published: December 2019

AbstractThis work presents the effect of chemical treatment of short jute fibers using alkali, potassium permanganate, and silane treatment on the mechanical properties of jute‐polypropylene (PP) composites. The chemically treated jute fibers were compounded at 30% weight fraction with PP granules in twin‐screw extruder, and the composite pellets were later injection molded to obtain the specimens for the study. The optimum parameters for the respective chemical treatments used in this study were followed as per the literature. The effectiveness of the chemical treatment carried out was studied using Fourier transform infrared spectroscopy and scanning electron microscopy. The mechanical characterization comprising tensile test, flexural test, impact test, and microhardness test were conducted on jute‐PP composites. In addition, the effect of chemical treatment on the water absorption kinetics of the jute‐PP composites was studied. The diffusion coefficient of the jute‐PP composite was determined using the approximate solution of Fick's model. The silane‐treated jute‐PP composites show an increase in tensile strength, tensile modulus, and flexural modulus by nearly 6%, 22%, and 20%, respectively, compared to untreated jute‐PP composite. The moisture absorption study reveals that the silane‐treated jute‐PP composite is the most effective.
The reactivity of surface modification of hydroxyapatite particles depends on their shape
Authors: Li Jie , Wang Jinfeng , Yang Shuang , Cao Yang , Yang Fang , Wu Jinchuan 
Published: December 2019

AbstractThe effect of hydroxyapatite (HA) particle shape on the reactivity of HA surfaces was investigated by selecting the rod‐shaped (r‐HA) and spherical HA particles (s‐HA) to coinitiate the ring‐opening polymerization of d,l‐lactide (DLLA) used stannous octoate (Sn(Oct)2) as the catalyst, respectively. It was found that both s‐HA and r‐HA could be grafted with PDLLA and the reactivity of s‐HA was higher than that of r‐HA according to the results from FTIR, NMR, TGA and BET method. The potential mechanism was that the shape of HA particles determined the crystal faces exposed to participate in graft reaction clarified by performing X‐ray diffraction detections and Materials Studio analysis. The primary crystal faces were (211) and (112) for s‐HA yet (002) and (300) for r‐HA. These findings suggested that the shape of HA particles might influence their reactivity, which might provide a new way to improve the graft ratio of polymers so as to prepare HA/polymer composites with strong interfacial strength by selecting or designing the shape of the HA particles.
Berberine induces resistance against tobacco mosaic virus in tobacco
Authors: Guo Wenhui , Yan He , Ren Xingyu , Tang Ruirui , Sun Yubo , Wang Yong , Feng Juntao 
Published: December 2019

AbstractBACKGROUNDPlant systemic resistance induced by botanical compounds is a promising alternative method of disease management. The natural product berberine, usually used as an antimicrobial in medicine, has been proven to have antifungal activity in agriculture. To investigate the induced resistance imparted by berberine, the effect of berberine against tobacco mosaic virus (TMV) and the mechanism governing this effect were determined.RESULTBerberine exhibited considerable in vivo anti‐TMV activity of up to 68.3% but had no in vitro direct effect on TMV. Moreover, berberine could induce immune responses against TMV in tobacco, including the hypersensitive reaction (HR), accumulation of H2O2, increases in defense enzymes and overexpression of pathogenesis‐related (PR) proteins. In addition, upregulation of salicylic acid (SA) biosynthesis genes PAL, CM1, ICS, PBS3 and the enzyme benzoic acid 2‐hydroxylase (BA2H) confirmed that SA was involved in the defensive signals. Berberine can induce crop resistance against TMV, Phytophthora nicotianae, Botrytis cinerea and Blumeria graminis in the greenhouse.CONCLUSIONThis paper highlights the use of berberine in manipulating tobacco to generate defense responses against TMV, which can be attributed to SA‐mediated induced resistance. The paper provides a theoretical basis for the application of berberine as a resistance activator and for further research on induced resistance by botanical natural product. © 2019 Society of Chemical Industry