bluuebunny/crossref_metadata_2025_split huggingface dataset:
Characteristics of Negative Leader Propagation Area of Lightning Flashes Initiated in the Stratiform Regions of Mesoscale Convective Systems
Authors: Wang Fei , Zhang Yijun , Dong Wansheng , Liu Hengyi , Li Feng , Yao Wen 
Published: December 2020

AbstractTo investigate the characteristics of extension areas (mainly the propagation areas of negative leaders in this study), the lightning location data of 254 lightning flashes initiated in the stratiform regions (stratiform lightning flashes) of 14 mesoscale convective systems (MCSs) are analyzed. The results show that most of the flashes have a relatively small lightning area (LA) (≤100 km2), although they are initiated in the stratiform regions. In small or developing MCSs, most negative leaders of stratiform lightning flashes concentrate within the 9–12‐km altitude range. In other MCSs with a large‐sized and developed stratiform region, besides being in this high‐altitude range, the negative leaders are also found to propagate more frequently in a low‐altitude range of 5–7 km. Further analysis indicates that most of the stratiform lightning flashes with a large LA (>100 km2) propagate their negative leaders within the high‐altitude range, no matter where they are initiated. Moreover, the stratiform lightning flashes with or near the largest LA tend to be initiated 4–6 km below their negative leaders, while most of the stratiform lightning flashes usually propagate their negative leaders horizontally within ±1 km of the first detected very‐high‐frequency (VHF) radiation source. It is inferred that some in‐situ electrifications occurring before and during the formation of the high reflectivity layers in the low‐altitude range contribute to these flashes, although the influence of the advection charges from the convective regions still cannot be totally ruled out.
Rare Treatable Cause of Demyelinating Leukoencephalopathy That One Cannot Afford to Miss
Authors: Gowda Vykuntaraju K. , Vignesh Sukanya , Nagarajan Balamurugan , Srinivasan Varunvenkat M. , Battina Manojna , Bhat Maya , Christopher Rita 
Published: December 2020

AbstractBiotinidase deficiency is a treatable neurometabolic disorder. It usually presents during the first year of life with seizures, ataxia, hypotonia, vision and hearing disturbance, alopecia, and skin rashes. It can have various neuroimaging findings but demyelinating leukoencephalopathy is an unusual finding in children with biotinidase deficiency that can cause diagnostic challenge as it can radiologically mimic perinatal hypoxic–ischemic encephalopathy or other leukodystrophies. It reverses with early diagnosis and treatment with biotin supplementation and the outcome is rewarding.
Tubal Flushing Treatment for Unexplained Infertility
Authors: Welie Nienke van , Ludwin Artur , Martins Wellington P. , Mijatovic Velja , Dreyer Kim 
Published: December 2020

AbstractTubal patency testing was initially introduced as a diagnostic test. However, it has been observed that some tubal patency tests also have a therapeutic effect. This therapeutic effect can be influenced by the contrast medium used during tubal flushing. In this review, we discuss current evidence associated with different methods for tubal flushing and their potential impact on reproductive outcomes in women with unexplained infertility. Furthermore, we discuss their diagnostic accuracy, safety, and cost-effectiveness.
The My Active and Healthy Aging ICT platform prevents quality of life decline in older adults: a randomised controlled study
Authors: Rainero Innocenzo , Summers Mathew J , Monter Michaela , Bazzani Marco , Giannouli Eleftheria , Aumayr Georg , Burin Dalila , Provero Paolo , Vercelli Alessandro E ,   for the My-AHA Consortium
Published: December 2020

Abstract

Introduction
Prevention of frailty is paramount in older adults. We evaluated the efficacy of a tailored multidomain intervention, monitored with the My Active and Healthy Aging platform, in reducing conversion from a prefrail status to overt frailty and preventing decline in quality of life.

Methods
We performed a multicentre, multicultural, randomised control study. The effects of multidomain interventions on frailty parameters, quality of life, physical, cognitive, psychosocial function, nutrition and sleep were evaluated in a group of 101 prefrail older subjects and compared with 100 prefrail controls, receiving general health advice.

Results
At the 12-month assessment, controls showed a decline in quality of life that was absent in the active group. In addition, active participants showed an increase in mood and nutrition function. No effect on remaining parameter was observed.

Discussion
Our study supports the use of personalised multidomain intervention, monitored with an information and communication technology platform, in preventing quality of life decline in older adults.
Metaphors, Judicial Frames, and Fundamental Rights in Cyberspace
Authors: Morelli Alessandro , Pollicino Oreste 
Published: December 2020

Abstract
How do legal imagination, metaphors, and the “judicial frame” impact the degree of protection for free expression when the relevant (technological) playground is the world of bits? This Article analyzes the so-called judicial frame, focusing on legal disputes relating to freedom of expression on the Internet. The authors compare the European Court of Human Rights and the U.S. Supreme Court case law from a methodological perspective. The Article shows how the adoption by supreme courts of an internal or external point of view in relation to the Internet affects not only the use of different metaphors to describe the digital world, but also the balance struck between the fundamental rights at stake.
Preparing pharmacists to increase naloxone dispensing within community pharmacies under the Pennsylvania standing order
Authors: Santa Heather M , Amirova Samira G , Ventricelli Daniel J , Downs George E , Nowalk Alexandra A , Pringle Janice L , Aruru Meghana 
Published: December 2020

Abstract

Purpose
Opioid misuse and overdose deaths remain a public health concern in the United States. Pennsylvania has one of the highest rates of opioid overdose deaths in the country, with Philadelphia County’s being 3 times higher than the national average. Despite several multimodal interventions, including use of SBIRT (screening, brief intervention, and referral to treatment) methods and naloxone distribution, the rate of overdose deaths remains high.

Methods
To gain insights on strategies for improving access to naloxone and naloxone distribution by pharmacists in Philadelphia County, a study was conducted in 11 community pharmacies (chain and independent) in Philadelphia. Twenty-four pharmacists were recruited and completed SBIRT and naloxone trainings. Each pharmacy elected to have at least 1 pharmacy champion who received additional training on and helped develop pharmacy site–specific naloxone dispensing protocols.

Results
Pre-post survey results showed a reduction in stigmatizing attitudes regarding naloxone dispensing and an increase in pharmacists’ understanding of the standing order and appropriate naloxone use. There was an increase in pharmacists’ self-reported confidence in their ability to appropriately identify, discuss, and dispense naloxone to patients. All pharmacies increased their average monthly dispensing rate following protocol implementation.

Conclusion
Pharmacists who received both trainings were more likely to change naloxone dispensing practices, leading to an overall increase in naloxone dispensing by community pharmacists. The study addressed overall gaps in pharmacists’ knowledge, reduced stigma, and prepared pharmacists to address opioid use and overdose prevention with their patients. The described pharmacist-led patient counseling and intervention service for overdose prevention may be explored as a model for other community pharmacies to adopt to improve naloxone dispensing and similar interventions to reduce overdose deaths.
A New Device for Autologous Small Volume Fat Grafting
Authors: Yang Xiaonan , Brower Jonathan P , Kokai Lauren , Gusenoff Beth R , Gusenoff Jeffrey A 
Published: December 2020

AbstractBackgroundMany techniques and devices have been developed to improve small volume fat grafting efficiency and consistency for use in the operating room and outpatient procedure room. These methods require cumbersome or messy processing to remove excess oil and fluid from adipose graft.ObjectivesThe aim of this study was to compare the fat processing efficiency of a novel handheld device, the Push-to-Spin (P2S) system, with that of other common processing techniques and validate tissue quality after lipoaspirate processing.MethodsHuman lipoaspirate samples were processed by 1 of 3 methods: cotton gauze (Telfa) rolling, centrifugation (Coleman technique), or the P2S system. Efficiency of fat processing was evaluated in terms of total processing time, fat harvest ratio, and fat processed ratio. Histologic examination and immunohistochemical staining were used to compare tissue morphology and adipocyte viability, respectively. Experimental samples were compared with unprocessed lipoaspirate controls.ResultsLipoaspirate processing was significantly faster with the P2S device than with other techniques. All 3 methods achieved similar fat harvest and fat processing ratios. Additionally, the P2S, Telfa, and Coleman techniques yielded grafts with similar cellularity and with similar perilipin and glycerol-3-phosphate dehydrogenase 1 expression. Measured differences between experimental and control samples were statistically significant.ConclusionsThe P2S device is an easy-to-use, efficient, and potentially cost-effective handheld device that can be used for lipoaspirate harvest, processing, and grafting in any procedural setting. The resulting adipocytes have similar morphology, viability, and function to those yielded by other techniques. This handheld technology decreases procedure time, thereby improving surgeon efficiency and patient experience.
Rapid and real-time identification of fungi up to species level with long amplicon nanopore sequencing from clinical samples
Authors: D’Andreano Sara , Cuscó Anna , Francino Olga 
Published: December 2020

Abstract
The availability of long-read technologies, like Oxford Nanopore Technologies, provides the opportunity to sequence longer fragments of the fungal ribosomal operon, up to 6 Kb (18S-ITS1-5.8S-ITS2-28S) and to improve the taxonomy assignment of the communities up to species level and in real-time. We assess the applicability for taxonomic assignment of amplicons targeting a 3.5 Kb region (V3 18S-ITS1-5.8S-ITS2-28S D2) and a 6 Kb region (V1 18S-ITS1-5.8S-ITS2-28S D12) with the What’s in my pot (WIMP) classifier. We used the ZymoBIOMICSTM mock community and different microbiological fungal cultures as positive controls. Long amplicon sequencing correctly identified Saccharomyces cerevisiae and Cryptococcus neoformans from the mock community and Malassezia pachydermatis, Microsporum canis and Aspergillus fumigatus from the microbiological cultures. Besides, we identified Rhodotorula graminis in a culture mislabelled as Candida spp. We applied the same approach to external otitis in dogs. Malassezia was the dominant fungal genus in dogs’ ear skin, whereas Ma. pachydermatis was the main species in the healthy sample. Conversely, we identified a higher representation of Ma. globosa and Ma. sympodialis in otitis affected samples. We demonstrate the suitability of long ribosomal amplicons to characterize the fungal community of complex samples, either healthy or with clinical signs of infection.
Cooking-energy transition in Nepal: trend review
Authors: Paudel Dipti , Jeuland Marc , Lohani Sunil Prasad 
Published: December 2020

Abstract
Clean-cooking energy is key to meeting climate-mitigation goals and a range of development objectives, especially for improving the well-being of women and children. Inefficient burning of solid biomass for cooking releases household air pollution that is hazardous to health, while putting pressure on forest resources. This paper provides an overview of the household-cooking-energy transition in Nepal to date. Despite numerous efforts by the government and other actors to speed this transition, energy data spanning the years 2000–18 reveal that ~69% of households nationwide still rely on solid fuels for cooking today. The proportion of solid-fuel users is especially high in rural regions, reaching &gt;80%. Moreover, if the current rate of progress is not accelerated, the use of solid fuels will remain high even in 2030, preventing the achievement of Sustainable Development Goal (SDG) 7. Cooking-fuel choices are heavily constrained by accessibility, demographic and socio-economic factors. Thus, this paper recommends that evidence-based and integrated policies and strategies be urgently deployed to foster a more effective and rapid transition towards clean energy, which is critical to achieving SDG 7.
When should we use mesh in laparoscopic hiatal hernia repair? A systematic review
Authors: Laxague Francisco , Sadava Emmanuel E , Herbella Fernando , Schlottmann Francisco 
Published: December 2020

Summary
The use of mesh in laparoscopic hiatal hernia repair (LHHR) remains controversial. The aim of this systematic review was to determine the usefulness of mesh in patients with large hiatal hernia (HH), obesity, recurrent HH, and complicated HH. We performed a systematic review of the current literature regarding the outcomes of LHHR with mesh reinforcement. All articles between 2000 and 2020 describing LHHR with primary suturing, mesh reinforcement, or those comparing both techniques were included. Symptom improvement, quality of life (QoL) improvement, and recurrence rates were evaluated in patients with large HH, obesity, recurrent HH, and complicated HH. Reported outcomes of the use of mesh in patients with large HH had wide variability and heterogeneity. Morbidly obese patients with HH should undergo a weight-loss procedure. However, the benefits of HH repair with mesh are unclear in these patients. Mesh reinforcement during redo LHHR may be beneficial in terms of QoL improvement and hernia recurrence. There is scarce evidence supporting the use of mesh in patients undergoing LHHR for complicated HH. Current data are heterogeneous and have failed to find significant differences when comparing primary suturing with mesh reinforcement. Further research is needed to determine in which patients undergoing LHHR mesh placement would be beneficial.
Should a Poverty-Averse Donor Always Reward Better Governance? A Paradox of Aid Allocation
Authors: Bourguignon François , Platteau Jean-Philippe 
Published: December 2020

Abstract
This article revisits the inter-country aid allocation by a donor who must distribute a given aid amount and is sensitive to needs and governance considerations. Against conventional wisdom, if the donor has strong enough aversion to poverty, the share of a country whose governance has improved is reduced. Yet, the poor will still be better off. These results continue to hold when aid effectiveness depends on intrinsic governance and the volume of aid received, and when a more general dynamic specification is considered. Finally, using our approach, the allocation rules in international organisations appear as clearly privileging governance over needs.
Biochemical characterization of two chitinases from Bacillus cereus sensu lato B25 with antifungal activity against Fusarium verticillioides P03
Authors: Morales-Ruiz Estefanía , Priego-Rivera Ricardo , Figueroa-López Alejandro Miguel , Cazares-Álvarez Jesús Eduardo , Maldonado-Mendoza Ignacio E 
Published: December 2020

ABSTRACT
Bacterial chitinases are a subject of intense scientific research due to their biotechnological applications, particularly their use as biological pesticides against phytopathogenic fungi as a green alternative to avoid the use of synthetic pesticides. Bacillus cereus sensu lato B25 is a rhizospheric bacterium that is a proven antagonist of Fusarium verticillioides, a major fungal pathogen of maize. This bacterium produces two chitinases that degrade the fungal cell wall and inhibit its growth. In this work, we used a heterologous expression system to purify both enzymes to investigate their biochemical traits in terms of Km, Vmax, optimal pH and temperature. ChiA and ChiB work as exochitinases, but ChiB exhibited a dual substrate activity and it is also an endochitinase. In this work, the direct addition of these chitinases inhibited fungal conidial germination and therefore they may play a major role in the antagonism against F. verticillioides.
Machine-learning-based detection of volcano seismicity using the spatial pattern of amplitudes
Authors: Maeda Yuta , Yamanaka Yoshiko , Ito Takeo , Horikawa Shinichiro 
Published: December 2020

SUMMARYWe propose a new algorithm, focusing on spatial amplitude patterns, to automatically detect volcano seismic events from continuous waveforms. Candidate seismic events are detected based on signal-to-noise ratios. The algorithm then utilizes supervised machine learning to classify the existing candidate events into true and false categories. The input learning data are the ratios of the number of time samples with amplitudes greater than the background noise level at 1 s intervals (large amplitude ratios) given at every station site, and a manual classification table in which ‘true’ or ‘false’ flags are assigned to candidate events. A two-step approach is implemented in our procedure. First, using the large amplitude ratios at all stations, a neural network model representing a continuous spatial distribution of large amplitude probabilities is investigated at 1 s intervals. Second, several features are extracted from these spatial distributions, and a relation between the features and classification to true and false events is learned by a support vector machine. This two-step approach is essential to account for temporal loss of data, or station installation, movement, or removal. We evaluated the algorithm using data from Mt. Ontake, Japan, during the first ten days of a dense observation trial in the summit region (2017 November 1–10). Results showed a classification accuracy of more than 97 per cent.
Empowering Dutch and Surinamese children to prevent viral infections: implications from an international education module
Authors: Shoker Kifah , Doornekamp Laura , Horjus Bob , Wagener Marlies N , Aron Georgina I , Goeijenbier Marco , van Gorp Eric C M 
Published: December 2020

Summary
Viral infections have a large share in human morbidity and mortality. Next to vaccinations and hygiene measures, health education plays a role in preventing infections. Social scientists argue that empowerment should be included in health education, as increasing knowledge is insufficient to achieve sustainable behaviour change. Within the international education module ‘Viruskenner’, primary school students learn how to prevent virus infections by identifying health risks and developing interventions. This qualitative formative study explored to what extent Viruskenner creates conditions in which empowerment processes can arise and take place in the Netherlands and Suriname. Indicators of empowerment, as defined in the literature and placed in the attitude, social influence, and self-efficacy model, were assessed during semi-structured interviews (n = 24) with students, parents, teachers and facilitators. We conclude that Viruskenner is successful in creating conditions for empowerment processes to arise and take place, specifically in attitude and self-efficacy. According to interviewees, the module raised students’ motivation, skills and confidence to take action to improve health behaviour. Educators played a stimulating role in the participatory setting in both countries, while content relevance and community involvement differed between the Netherlands and Suriname. These outcomes could improve this module and possibly other health education programmes.
Reflection on modern methods: causal inference considerations for heterogeneous disease etiology
Authors: Nevo Daniel , Ogino Shuji , Wang Molin 
Published: December 2020

Abstract
Molecular pathological epidemiology research provides information about pathogenic mechanisms. A common study goal is to evaluate whether the effects of risk factors on disease incidence vary between different disease subtypes. A popular approach to carrying out this type of research is to implement a multinomial regression in which each of the non-zero values corresponds to a bona fide disease subtype. Then, heterogeneity in the exposure effects across subtypes is examined by comparing the coefficients of the exposure between the different subtypes. In this paper, we explain why this common method potentially cannot recover causal effects, even when all confounders are measured, due to a particular type of selection bias. This bias can be explained by recognizing that the multinomial regression is equivalent to a series of logistic regressions; each compares cases of a certain subtype to the controls. We further explain how this bias arises using directed acyclic graphs and we demonstrate the potential magnitude of the bias by analysis of a hypothetical data set and by a simulation study.
Prevalence and characterization of mecC MRSA in bovine bulk tank milk in Great Britain, 2017–18
Authors: Cui Cheng , Ba Xiaoliang , Holmes Mark A 
Published: December 2020

Abstract

Objectives
To evaluate the current prevalence status of mecC MRSA among dairy farms in England and Wales 5 years after a previous survey conducted in 2011–12.

Methods
A convenience sample of 697 dairy farms in England and Wales was used for the study, conducted in 2017–18, testing bulk tank milk samples for the presence of mecC MRSA using high salt broth enrichment and chromogenic MRSA agar selection. All putative MRSA isolates were screened by PCR for the presence of mecA and mecC genes and subjected to antimicrobial susceptibility testing using both the disc diffusion method and VITEK® 2. MRSA isolates were also sequenced for genomic characterization.

Results
mecC MRSA were detected on 4 out of 697 dairy farms in England and Wales (prevalence 0.57%, 95% CI 0.16%–1.46%). Three of the mecC isolates were ST425 and one was ST4652 (in the CC130 lineage). Two mecA MRSA were also isolated: one ST5 and one ST398.

Conclusions
These results indicate that there has been a substantial reduction in the prevalence of mecC MRSA in England and Wales with a 72% reduction (2.15% to 0.57%) compared with a previous study. While the levels of mecA MRSA remain very low the continued presence of ST398, a livestock-associated MRSA, suggests that this lineage is established in the UK.