bluuebunny/crossref_metadata_2025_split huggingface dataset:
Shifts in Phytoplankton Composition and Stepwise Climate Change During the Middle Miocene
Authors: Henderiks J. , Bartol M. , Pige N. , Karatsolis B.‐Th. , Lougheed B. C. 
Published: July 2020

AbstractThe abundance and composition of modern phytoplankton are primarily related to equator‐to‐pole temperature gradients and global ocean circulation, which in turn determine the availability of nutrients in the photic zone. The nutricline is found at greater depths in warm, tropical waters, whereas more vigorous surface mixing in higher latitudes (seasonally) enhances nutrient availability and primary productivity. Ocean temperatures were ~7°C higher during the middle Miocene Climatic Optimum (MCO; ~16.9–14.7 million years ago, Ma), which was followed by Antarctic glaciation and global cooling during the middle Miocene Climate transition (MMCT; 14.7–13.8 Ma). Four decades ago, Haq (1980, https://doi.org.10.2307/1485353) already related migration patterns of low‐latitude versus high‐latitude calcareous nannoplankton in the Atlantic Ocean to major climatic fluctuations during the Miocene. Here, we detail and discuss the macroevolutionary patterns and processes across the middle Miocene (~16.5–11 Ma) at five deep sea sites on a north‐south transect in the Atlantic Ocean (57°N to 28°S). We show that the major cooling step toward the modern “icehouse” world impacted coccolithophore communities at all latitudes. Contrary to previous observations suggesting that tropical sites showed little change and that midlatitudes were the most sensitive recorders of climate change across the MMCT, we show that all sites recorded a marked diversification and increase in abundance of reticulofenestrids. Global cooling and related increased meridional overturning circulation are implicated as likely forcings for this macroevolutionary step toward establishing modern coccolithophore communities that are dominated by eurythermal and eurytrophic species such as Emiliania huxleyi.
Geostatistical Distribution of Vertical Refractivity Gradient Over Nigeria
Authors: Emmanuel I. , Ojo O. S. , Abe O. E. , Adedayo K. D. 
Published: July 2020

AbstractAnomalous propagation conditions may cause multipath fading and strong signal enhancement on terrestrial line of sight links or interference on transhorizon paths. In detecting this conditions, spatial distribution of vertical refractivity gradient and ducting index were estimated across Nigeria using atmospheric data obtained from Era5 archive of European Centre for Medium‐Range Weather Forecasts (ECMWF). Refractivity and refractivity gradient were calculated from the obtained temperature, relative humidity, and pressure data using International Telecommunication Union (ITU) recommendation expression. Vertical refractivity gradient (VRG) and ducting index (Di) were deducted from refractivity gradient and modified the refractivity gradient. Correlation between vertical refractivity gradient, Di, and latitude were also computed. The result shows that the VRG values range between −5 and −65 N units/km in the Coastal and Guinea Savannah regions, and between −30 and −95 N units/km in the Midland and Sahelian regions. The Di values range between 10 and 60 in the Coastal and Guinea Savannah regions, between −10 and 110 in Midland and Sahelian region. Likewise, superrefraction and ducting are more prominent in the northern part, while superrefraction with minor subrefraction were noticed in the south. The Di performed better than VRG in detecting propagation conditions. Correlation between VRG and latitude ranges between −0.75 and −0.92. Correlation between Di and latitude is between 0.84 and 0.95. The values of VRG decrease northward, while those of the Di increase northward. Both VRG and Di revealed high variability in the dry months than in the wet months.
Progesterone receptor membrane component 1 promotes the growth of breast cancers by altering the phosphoproteome and augmenting EGFR/PI3K/AKT signalling
Authors: Pedroza Diego A. , Rajamanickam Venkatesh , Subramani Ramadevi , Bencomo Alejandra , Galvez Adriana , Lakshmanaswamy Rajkumar 
Published: July 2020

Abstract
Background
Increased expression of the progesterone receptor membrane component 1 (PGRMC1) has been linked to multiple cancers, including breast cancer. Despite being a regulatory receptor and a potential therapeutic target, the oncogenic potential of PGRMC1 has not been studied.

Methods
The impact of PGRMC1 on breast cancer growth and progression was studied following chemical inhibition and alteration of PGRMC1 expression, and evaluated by using online-based gene expression datasets of human breast cancer tissue. MTS, flow cytometry, qPCR, Western blotting, confocal microscopy and phosphoproteome analysis were performed.

Results
We observed higher PGRMC1 levels in both ER-positive ZR-75-1 and TNBC MDA-MB-468 cells. Both chemical inhibition and silencing decreased cell proliferation, induced cell-cycle arrest, promoted apoptosis and reduced the migratory and invasive capabilities of ZR-75-1 and MDA-MB-468 cells. Further, phosphoproteome analysis demonstrated an overall decrease in activation of proteins involved in PI3K/AKT/mTOR and EGFR signalling pathways. In contrast, overexpression of PGRMC1 in non-malignant MCF10A cells resulted in increased cell proliferation, and enhanced activity of PI3K/AKT/mTOR and EGFR signalling pathways.

Conclusions
Our data demonstrate that PGRMC1 plays a prominent role in regulating the growth of cancer cells by altering the PI3K/AKT/mTOR and EGFR signalling mechanisms in both ER-positive and TNBC cells.
Therapeutic effects of rapamycin and surgical decompression in a rabbit spinal cord injury model
Authors: Zhang Xin , Qin Chuan , Jing Yingli , Yang Degang , Liu Changbin , Gao Feng , Zhang Chao , Talifu Zuliyaer , Yang Mingliang , Du Liangjie , Li Jianjun 
Published: July 2020

AbstractSurgical decompression after spinal cord injury (SCI) is a conventional treatment. Although it has been proven to have clinical effects, there are certain limitations, such as the surgical conditions that must be met and the invasive nature of the treatment. Therefore, there is an urgent need to develop a simple and maneuverable therapy for the emergency treatment of patients with SCI before surgery. Rapamycin (RAPA) has been reported to have potential as a therapeutic agent for SCI. In this study, we observed the therapeutic effects of rapamycin and surgical decompression, in combination or separately, on the histopathology in rabbits with SCI. After combination therapy, intramedullary pressure (IMP) decreased significantly, autophagic flux increased, and apoptosis and demyelination were significantly reduced. Compared with RAPA/surgical decompression alone, the combination therapy had a significantly better effect. In addition, we evaluated the effects of mechanical pressure on autophagy after SCI by assessing changes in autophagic initiation, degradation, and flux. Increased IMP after SCI inhibited autophagic degradation and impaired autophagic flux. Decompression improved autophagic flux after SCI. Our findings provide novel evidence of a promising strategy for the treatment of SCI in the future. The combination therapy may effectively improve emergency treatment after SCI and promote the therapeutic effect of decompression. This study also contributes to a better understanding of the effects of mechanical pressure on autophagy after neurotrauma.
Photoactivatable oncolytic adenovirus for optogenetic cancer therapy
Authors: Hagihara Yasuko , Sakamoto Ayaka , Tokuda Takashi , Yamashita Tomoki , Ikemoto Sena , Kimura Ayaka , Haruta Makito , Sasagawa Kiyotaka , Ohta Jun , Takayama Kazuo , Mizuguchi Hiroyuki 
Published: July 2020

AbstractVirotherapy using oncolytic adenovirus is an effective anticancer strategy. However, the tumor selectivity of oncolytic adenoviruses is not enough high. To develop oncolytic adenovirus with a low risk of off-tumor toxicity, we constructed a photoactivatable oncolytic adenovirus (paOAd). In response to blue light irradiation, the expression of adenoviral E1 genes, which are necessary for adenoviral replication, is induced and replication of this adenovirus occurs. In vitro, efficient lysis of various human cancer cell lines was observed by paOAd infection followed by blue light irradiation. Importantly, there was no off-tumor toxicity unless the cells were irradiated by blue light. In vivo, tumor growth in a subcutaneous tumor model and a mouse model of liver cancer was significantly inhibited by paOAd infection followed by blue light irradiation. In addition, paOAd also showed a therapeutic effect on cancer stem cells. These results suggest that paOAd is useful as a safe and therapeutically effective cancer therapy.
Galectin-3 deficiency in pregnancy increases the risk of fetal growth restriction (FGR) via placental insufficiency
Authors: Freitag Nancy , Tirado-Gonzalez Irene , Barrientos Gabriela , Powell Katie L. , Boehm-Sturm Philipp , Koch Stefan P. , Hecher Kurt , Staff Anne C. , Arck Petra C. , Diemert Anke , Blois Sandra M. 
Published: July 2020

AbstractFetal growth restriction (FGR) is the most common pregnancy complication in developed countries. Pregnancies affected by FGR, frequently concur with complications and high risk of neonatal morbidity and mortality. To date, no approved treatment is available for pregnant women affected with FGR. The objective of this study was to investigate the contribution of galectin-3 (gal-3), a β-galactoside binding protein involved in pregnancy, placental function and fetal growth. We demonstrated that lack of gal-3 during mouse pregnancy leads to placental dysfunction and drives FGR in the absence of a maternal preeclampsia syndrome. Analysis of gal-3 deficient dams revealed placental inflammation and malperfusion, as well as uterine natural killer cell infiltration with aberrant activation. Our results also show that FGR is associated with a failure to increase maternal circulating gal-3 levels during the second and third trimester in human pregnancies. Placentas from human pregnancies affected by FGR displayed lower gal-3 expression, which correlated with placental dysfunction. These data highlight the importance of gal-3 in the promotion of proper placental function, as its absence leads to placental disease and subsequent FGR.
Bile acids drive the newborn’s gut microbiota maturation
Authors: van Best N. , Rolle-Kampczyk U. , Schaap F. G. , Basic M. , Olde Damink S. W. M. , Bleich A. , Savelkoul P. H. M. , von Bergen M. , Penders J. , Hornef M. W. 
Published: July 2020

AbstractFollowing birth, the neonatal intestine is exposed to maternal and environmental bacteria that successively form a dense and highly dynamic intestinal microbiota. Whereas the effect of exogenous factors has been extensively investigated, endogenous, host-mediated mechanisms have remained largely unexplored. Concomitantly with microbial colonization, the liver undergoes functional transition from a hematopoietic organ to a central organ of metabolic regulation and immune surveillance. The aim of the present study was to analyze the influence of the developing hepatic function and liver metabolism on the early intestinal microbiota. Here, we report on the characterization of the colonization dynamics and liver metabolism in the murine gastrointestinal tract (n = 6–10 per age group) using metabolomic and microbial profiling in combination with multivariate analysis. We observed major age-dependent microbial and metabolic changes and identified bile acids as potent drivers of the early intestinal microbiota maturation. Consistently, oral administration of tauro-cholic acid or β-tauro-murocholic acid to newborn mice (n = 7–14 per group) accelerated postnatal microbiota maturation.
Zeta Inhibitory Peptide attenuates learning and memory by inducing NO-mediated downregulation of AMPA receptors
Authors: Bingor Alexey , Haham Tomer , Thornton Claire , Stern-Bach Yael , Yaka Rami 
Published: July 2020

AbstractZeta inhibitory peptide (ZIP), a PKMζ inhibitor, is widely used to interfere with the maintenance of acquired memories. ZIP is able to erase memory even in the absence of PKMζ, via an unknown mechanism. We found that ZIP induces redistribution of the AMPARGluA1 in HEK293 cells and primary cortical neurons, and decreases AMPAR-mediated currents in the nucleus accumbens (NAc). These effects were mimicked by free arginine or by a modified ZIP in which all but the arginine residues were replaced by alanine. Redistribution was blocked by a peptidase-resistant version of ZIP and by treatment with the nitric oxide (NO)-synthase inhibitor L-NAME. ZIP increased GluA1-S831 phosphorylation and ZIP-induced redistribution was blocked by nitrosyl-mutant GluA1-C875S or serine-mutant GluA1-S831A. Introducing the cleavable arginine-alanine peptide into the NAc attenuated expression of cocaine-conditioned reward. Together, these results suggest that ZIP may act as an arginine donor, facilitating NO-dependent downregulation of AMPARs, thereby attenuating learning and memory.
Gasdermin-D-dependent IL-1α release from microglia promotes protective immunity during chronic Toxoplasma gondii infection
Authors: Batista Samantha J. , Still Katherine M. , Johanson David , Thompson Jeremy A. , OʼBrien Carleigh A. , Lukens John R. , Harris Tajie H. 
Published: July 2020

AbstractMicroglia, resident immune cells of the CNS, are thought to defend against infections.Toxoplasma gondiiis an opportunistic infection that can cause severe neurological disease. Here we report that duringT. gondiiinfection a strong NF-κB and inflammatory cytokine transcriptional signature is overrepresented in blood-derived macrophages versus microglia. Interestingly, IL-1α is enriched in microglia and IL-1β in macrophages. We find that mice lacking IL-1R1 or IL-1α, but not IL-1β, have impaired parasite control and immune cell infiltration within the brain. Further, we show that microglia, not peripheral myeloid cells, release IL-1α ex vivo. Finally, we show that ex vivo IL-1α release is gasdermin-D dependent, and that gasdermin-D and caspase-1/11 deficient mice show deficits in brain inflammation and parasite control. These results demonstrate that microglia and macrophages are differently equipped to propagate inflammation, and that in chronicT. gondiiinfection, microglia can release the alarmin IL-1α, promoting neuroinflammation and parasite control.
Enhanced multi-carbon alcohol electroproduction from CO via modulated hydrogen adsorption
Authors: Li Jun , Xu Aoni , Li Fengwang , Wang Ziyun , Zou Chengqin , Gabardo Christine M. , Wang Yuhang , Ozden Adnan , Xu Yi , Nam Dae-Hyun , Lum Yanwei , Wicks Joshua , Chen Bin , Wang Zhiqiang , Chen Jiatang , Wen Yunzhou , Zhuang Taotao , Luo Mingchuan , Du Xiwen , Sham Tsun-Kong , Zhang Bo , Sargent Edward H. , Sinton David 
Published: July 2020

AbstractMulti-carbon alcohols such as ethanol are valued as fuels in view of their high energy density and ready transport. Unfortunately, the selectivity toward alcohols in CO2/CO electroreduction is diminished by ethylene production, especially when operating at high current densities (>100 mA cm−2). Here we report a metal doping approach to tune the adsorption of hydrogen at the copper surface and thereby promote alcohol production. Using density functional theory calculations, we screen a suite of transition metal dopants and find that incorporating Pd in Cu moderates hydrogen adsorption and assists the hydrogenation of C2 intermediates, providing a means to favour alcohol production and suppress ethylene. We synthesize a Pd-doped Cu catalyst that achieves a Faradaic efficiency of 40% toward alcohols and a partial current density of 277 mA cm−2 from CO electroreduction. The activity exceeds that of prior reports by a factor of 2.
Breaking the trade-off between fast control and long lifetime of a superconducting qubit
Authors: Kono S. , Koshino K. , Lachance-Quirion D. , van Loo A. F. , Tabuchi Y. , Noguchi A. , Nakamura Y. 
Published: July 2020

AbstractThe rapid development in designs and fabrication techniques of superconducting qubits has made coherence times of qubits longer. In the future, however, the radiative decay of a qubit into its control line will be a fundamental limitation, imposing a trade-off between fast control and long lifetime of the qubit. Here, we break this trade-off by strongly coupling another superconducting qubit along the control line. This second qubit, which we call “Josephson quantum filter” (JQF), prevents the first qubit from emitting microwave photons and thus suppresses its relaxation, while transmitting large-amplitude control microwave pulses due to the saturation of the quantum filter, enabling fast qubit control. This device functions as an automatic decoupler between a qubit and its control line and could help in the realization of a large-scale superconducting quantum processor by reducing the heating of the qubit environment and the crosstalk between qubits.
Fast growth of large-grain and continuous MoS2 films through a self-capping vapor-liquid-solid method
Authors: Chang Ming-Chiang , Ho Po-Hsun , Tseng Mao-Feng , Lin Fang-Yuan , Hou Cheng-Hung , Lin I-Kuan , Wang Hsin , Huang Pin-Pin , Chiang Chun-Hao , Yang Yueh-Chiang , Wang I-Ta , Du He-Yun , Wen Cheng-Yen , Shyue Jing-Jong , Chen Chun-Wei , Chen Kuei-Hsien , Chiu Po-Wen , Chen Li-Chyong 
Published: July 2020

AbstractMost chemical vapor deposition methods for transition metal dichalcogenides use an extremely small amount of precursor to render large single-crystal flakes, which usually causes low coverage of the materials on the substrate. In this study, a self-capping vapor-liquid-solid reaction is proposed to fabricate large-grain, continuous MoS2 films. An intermediate liquid phase-Na2Mo2O7 is formed through a eutectic reaction of MoO3 and NaF, followed by being sulfurized into MoS2. The as-formed MoS2 seeds function as a capping layer that reduces the nucleation density and promotes lateral growth. By tuning the driving force of the reaction, large mono/bilayer (1.1 mm/200 μm) flakes or full-coverage films (with a record-high average grain size of 450 μm) can be grown on centimeter-scale substrates. The field-effect transistors fabricated from the full-coverage films show high mobility (33 and 49 cm2 V−1 s−1 for the mono and bilayer regions) and on/off ratio (1 ~ 5 × 108) across a 1.5 cm × 1.5 cm region.
An active mechanical Willis meta-layer with asymmetric polarizabilities
Authors: Chen Yangyang , Li Xiaopeng , Hu Gengkai , Haberman Michael R. , Huang Guoliang 
Published: July 2020

AbstractWillis materials exhibit macroscopic cross-coupling between particle velocity and stress as well as momentum and strain. However, Willis coupling coefficients designed so far are intrinsically coupled, which inhibits their full implementation in structural dynamic applications. This work presents a means to eliminate these limitations by introducing an active scatterer in a mechanical meta-layer that exploits piezoelectric sensor–actuator pairs controlled by digital circuits. We experimentally demonstrate abilities of the Willis meta-layer, in beams and plates, for independently engineering transmission and reflection coefficients of flexural waves in both amplitude and phase and nonreciprocal wave propagations. The meta-layer is described by a flexural wave polarizability tensor, which captures independent higher-order symmetric-to-symmetric and symmetric-to-antisymmetric couplings. The active meta-layer is adaptive in real time for reconfigurable broadband operation thanks to its programmability. This work sheds a new light on unsurpassed control of elastic waves, ranging from vibration protections to ultrasonic sensing and evaluation of engineering structures.
Structural mechanism for gating of a eukaryotic mechanosensitive channel of small conductance
Authors: Deng Zengqin , Maksaev Grigory , Schlegel Angela M. , Zhang Jingying , Rau Michael , Fitzpatrick James A. J. , Haswell Elizabeth S. , Yuan Peng 
Published: July 2020

AbstractMechanosensitive ion channels transduce physical force into electrochemical signaling that underlies an array of fundamental physiological processes, including hearing, touch, proprioception, osmoregulation, and morphogenesis. The mechanosensitive channels of small conductance (MscS) constitute a remarkably diverse superfamily of channels critical for management of osmotic pressure. Here, we present cryo-electron microscopy structures of a MscS homolog from Arabidopsis thaliana, MSL1, presumably in both the closed and open states. The heptameric MSL1 channel contains an unusual bowl-shaped transmembrane region, which is reminiscent of the evolutionarily and architecturally unrelated mechanosensitive Piezo channels. Upon channel opening, the curved transmembrane domain of MSL1 flattens and expands. Our structures, in combination with functional analyses, delineate a structural mechanism by which mechanosensitive channels open under increased membrane tension. Further, the shared structural feature between unrelated channels suggests the possibility of a unified mechanical gating mechanism stemming from membrane deformation induced by a non-planar transmembrane domain.
High-resolution monthly precipitation and temperature time series from 2006 to 2100
Authors: Karger Dirk Nikolaus , Schmatz Dirk R. , Dettling Gabriel , Zimmermann Niklaus E. 
Published: July 2020

AbstractPredicting future climatic conditions at high spatial resolution is essential for many applications and impact studies in science. Here, we present monthly time series data on precipitation, minimum- and maximum temperature for four downscaled global circulation models. We used model output statistics in combination with mechanistic downscaling (the CHELSA algorithm) to calculate mean monthly maximum and minimum temperatures, as well as monthly precipitation at ~5 km spatial resolution globally for the years 2006–2100. We validated the performance of the downscaling algorithm by comparing model output with the observed climate of the historical period 1950–1969.
Identification of cell types from single cell data using stable clustering
Authors: Peyvandipour Azam , Shafi Adib , Saberian Nafiseh , Draghici Sorin 
Published: July 2020

AbstractSingle-cell RNA-seq (scRNASeq) has become a powerful technique for measuring the transcriptome of individual cells. Unlike the bulk measurements that average the gene expressions over the individual cells, gene measurements at individual cells can be used to study several different tissues and organs at different stages. Identifying the cell types present in the sample from the single cell transcriptome data is a common goal in many single-cell experiments. Several methods have been developed to do this. However, correctly identifying the true cell types remains a challenge. We present a framework that addresses this problem. Our hypothesis is that the meaningful characteristics of the data will remain despite small perturbations of data. We validate the performance of the proposed method on eight publicly available scRNA-seq datasets with known cell types as well as five simulation datasets with different degrees of the cluster separability. We compare the proposed method with five other existing methods: RaceID, SNN-Cliq, SINCERA, SEURAT, and SC3. The results show that the proposed method performs better than the existing methods.
Sub-half-cycle field transients from shock-wave-assisted soliton self-compression
Authors: Voronin A. A. , Zheltikov A. M. 
Published: July 2020

AbstractWe identify an unusual regime of ultrafast nonlinear dynamics in which an optical shock wave couples to soliton self-compression, steepening the tail of the pulse, thus yielding self-compressing soliton transients as short as the field sub-half-cycle. We demonstrate that this extreme pulse self-compression scenario can help generate sub-half-cycle mid-infrared pulses in a broad class of anomalously dispersive optical waveguide systems.