bluuebunny/crossref_metadata_2025_split huggingface dataset:
Making a case for preconception care—the pregnancy experiences of women with pre-existing medical conditions in Ibadan, Nigeria
Authors: Ojifinni Oludoyinmola Omobolade , Ibisomi Latifat 
Published: March 2020

Abstract
BackgroundFactors that predispose to poor maternal and child health outcomes in most low and middle income countries include the presence of pre-existing medical conditions that are amenable to preconception care (PCC). Nigeria has an increasing pool of women of reproductive age with pre-existing medical conditions but PCC services are not provided routinely in the maternal and child health framework. This study explores the pregnancy experiences of women with pre-existing medical conditions to make a case for PCC as a routine service.MethodsNine women purposively selected because they has pre-existing medical conditions participated in in-depth interviews (IDIs) in this qualitative study. The IDIs were held in the obstetric outpatient clinics and lying-in wards of two referral hospitals for maternal and child health services in Ibadan North LGA of Oyo State, southwest Nigeria. The interviews lasted an average of 30 minutes, were digitally recorded and transcribed verbatim. Thematic analysis using a hybrid of inductive and deductive coding was done using MAXQDA 2018.ResultsAmong the nine participants, seven were pregnant and two non-pregnant with pre-existing medical conditions including hypertension, diabetes mellitus, sickle cell disorder, chronic hepatitis, HIV, previous pregnancy loss of unknown cause and secondary infertility. None of the participants were aware of PCC and although they all desired their current pregnancy, there was no active preparation. None of the pregnant participants notified their health care providers about their desire for pregnancy and their medications were not adjusted or changed till after pregnancy. All except one of the participants believed they could have benefitted from PCC if they had been aware before pregnancy.ConclusionThe regular contact with the health system afforded by their pre-existing medical conditions is an opportunity for them to have been adequately prepared for pregnancy through counselling and adjustment or change in treatment regimen to prevent complications. This opportunity was missed among the study participants. Health care providers need to be proactive and ask women of reproductive age about their pregnancy desires during routine clinic visits in order to make adequate preparation.
Free Vaccination and Immunization Coverage of Uninsured Children: The Case of the Vaccines for Children Program in the United States
Authors: Kawase Akihiro 
Published: March 2020

Abstract
Objective : Vaccines against contagious diseases have strong positive externalities as immunization protects not just the immunized but those around them. Out-of-pocket immunization costs are a common barrier to obtaining vaccines, especially for low-income families or those without health insurance in the United States. The Vaccines for Children (VFC) Program, initiated in October 1994, allows all uninsured children in the United States to receive free vaccinations. Despite its importance, few studies have focused on the effectiveness of this program. Using data from the National Immunization Survey (NIS) from 1995–1997 (N=51902), this study investigates how the introduction of this program affected the immunization coverage of uninsured children aged 19–35 months.Results : Accounting for variation in a child’s exposure to the program, I found that providing free vaccination correlated with an increase in the uptake of the entire spectrum of recommended vaccines, which included hepatitis B (Hep b) vaccine added to the recommended immunization schedule at the time. Further, despite the introduction of the program, uninsured children continue to have low immunization coverage. These findings suggest that improving immunization coverage for uninsured children by only reducing out-of-pocket vaccination costs may be insufficient and other factors may still influence vaccination decisions.
A techno-practical method for overcoming the biotoxicity and volatility obstacles of butanol and butyric acid during whole-cell catalysis by Gluconobacter oxydans
Authors: Hua Xia , Du GenLai , Zhou Xin , Xu Yong 
Published: March 2020

Abstract
Background

Butyric acid is a platform chemical material, the production of which has been greatly stimulated by the diverse range of downstream applications in many industries. In particular, higher quality butyric acid used in food and medicine, is more dependent on microbiological production methods. Hence, the bio-oxidation of butanol to butyric acid has been identified as a promising method with good potential economic and environmental benefits. However, both butanol and butyric acid usually performs intensive toxicity on most microorganisms as well as the bio-oxidation pathway. To develop a green, efficient and competitive microbiological method is the primary work to overcome the bottleneck of butyric acid industry.

Result

A combined bioprocess was designed with alternative whole-cell catalysis for butyric acid bio-conversion from butanol by Gluconobacter oxydans in a sealed-oxygen supply bioreactor (SOS). In the operation system, the escape of volatile substrates and toxic chemicals to cells can be avoided by the use of a sealed bioreactor, combined with the rejuvenation of cells by supplying energy co-factors. Finally, during one batch whole-cell catalysis, the utilization rate of substrate increased from 56.6% to 96.0% by the simply skill. Additionally, the techno-practical bioprocess can realize the purpose of cell-recycling technology through the rejuvenation effect of co-factor. Finally, we obtained 135.3 g/L butyric acid and 216.7 g/L sorbose during 60 h whole-cell catalysis. This techno-practical technology provides a promising approach to promote the industrial production of butyric acid with more competitiveness.

Conclusion

The techno-practical biotechnology has powerfully promoted the process of butyric acid production by microorganism, especially makes up for the lack of aerobic fermentation in the industry, and surmount the shortcomings of traditional anerobic fermentation. At the same time, this technically-practical system provides a promising approach for the promotion of the industrial production of butyric acid in a more competitive manner.
Stress response after breastfeeding and breastfeeding self-efficacy as modifiable predictors of exclusive breastfeeding at 3 months postpartum: a longitudinal study
Authors: Shiraishi Mie , Matsuzaki Masayo , Kurihara Shoko , Iwamoto Maki , Shimada Mieko 
Published: March 2020

Abstract
Background: Only 50% of mothers in Japan exclusively breastfeed their infants during the postpartum period. To increase this rate, we aimed to examine modifiable factors at 1 month postpartum related to exclusive breastfeeding at 3 months postpartum by focusing on breastfeeding-related and psychosocial variables. Methods: This prospective cohort study was a secondary analysis of a longitudinal study, which was conducted in a secondary medical care center in Osaka, Japan from 2017 to 2018. Demographic variables, infant feeding modality, breastfeeding-related variables, and psychosocial variables were obtained using questionnaires at 1 month postpartum. Daytime salivary cortisol levels before and after breastfeeding at 1 month postpartum were measured as a biological marker for stress responses associated with breastfeeding. Each infant’s feeding modality was re-assessed at 3 months postpartum. A multiple logistic regression analysis was performed to examine the factors affecting exclusive breastfeeding at 3 months postpartum. Results: Of the 104 participants, 61 reported exclusive breastfeeding at 3 months postpartum. The following factors significantly affected exclusive breastfeeding at 3 months postpartum: multipara (adjusted odds ratio, 95% confidence interval: 11.128, 2.078–59.594), having a university degree (5.246, 1.037–26.526), no plan to return to work by 6 months postpartum (0.021, 0.001–0.460), exclusive breastfeeding at 1 month postpartum (42.841, 6.047–303.515), higher breastfeeding self-efficacy scale score at 1 month postpartum (1.070, 1.004–1.139), and lower cortisol level after breastfeeding at 1 month postpartum (0.000, 0.000–0.020). Conclusions: Stress levels after breastfeeding and breastfeeding self-efficacy were identified as modifiable factors related to subsequent exclusive breastfeeding. Healthcare professionals need to develop effective approaches to reducing breastfeeding-related stress and improving breastfeeding self-efficacy to help women fulfill their antenatal breastfeeding intentions and increase exclusive breastfeeding practices.
Application Effect of Reading List in Teaching of Temporal CT Reading
Authors: Wei Fanqin , Fang Ruihua , Wen Lanying , Qin Zili , Wu Xuan 
Published: March 2020

Abstract
Background

Temporal CT is the most commonly used imaging data in the clinical work of otology. How to cultivate and improve the ability of residents to read images has become an important focus in clinical teaching. Our aim is to explore the application effect of reading list in the reading teaching of temporal CT for resident otologists.

Methods

The resident otologists were selected as subjects and randomly divided into a Group A and a Group B. Group A adopted reading list of temporal CT while Group B did not adopted reading list. The ability of student to read temporal CT was tested and evaluated.

Results

The scores and identification proficiency of temporal CT in Group A were significantly better than those in Group B after training. The evaluation in the learning interest, prediction ability of surgical risk, prevention of missed diagnosis and misdiagnosis risk, doctor-patient communication ability, standard organization of reading, self-confidence in reading, self-learning of reading, and satisfaction degree with reading teaching methods in Group A were better than those in Group B.

Conclusion

Application of reading list can improve the ability of resident otologists to read temporal CT and predict surgical risk, and help students cultivate the standard reading habits and enhance their interest in learning.
Association of S. haematobium Infection Morbidity and Severity on Co-infections status in Pre-school Age Children Living in a Rural Endemic Area in Zimbabwe
Authors: Mduluza-Joko Tariro Lavender , Naicker Thajasvarie , Jokonya Luxwell , Midzi Herald , Vengesai Arthur , Kasambala Maritha , Choto Emelia , Rusakaniko Simbarashe , Sibanda Elopy , Mutapi Francisca , Mdul Takafira 
Published: March 2020

Abstract
Background: Individuals living in S. haematobium endemic areas are often at risk of having other communicable diseases, simultaneously. This usually creates diagnostic difficulties leading to misdiagnosis and overlooking schistosomiasis infection. In this study we investigated the prevalence and effects of coinfections in pre-school age children.Methodology : About 465 Preschool age children were clinically examined for the following top morbidity conditions: respiratory tract infections, dermatophytosis, malaria and fever of unknown origin. S. haematobium infection was diagnosed by urine filtration and the children were screened for other communicable infections common in rural areas.Results : Prevalence of S. haematobium was 35% (145). A positive relationship between S. haematobium prevalence and under-5 mortality rate in Zimbabwean provinces was demonstrated. The odds of co-infections observed for S. haematobium were: upper respiratory tract infection AOR = 1.98 (95% CI 1.657 to 2.48), dermatophytosis AOR = 5.10 ( 95% CI 2.99 to 8.72), fever of unknown origin AOR = 9.07 ( 95% CI 5.70 to 14.44) and malaria AOR = 0.91 ( 95% CI 0.54 to1.54). The risk ratio of having S. haematobium and co-infections in children who had fever of unknown origin 138%, dermatophytosis 38%, Upper respiratory tract infection 1% increase risk and malaria had a 2% reduced risk. Odds of having severe sequelae following the above conditions were: severe pneumonia AOR = 8.41(95%CI 3.09-22.93), complicated malaria AOR = 7.09 (95% CI 1.51-33.39), severe and persistent dermatophytosis AOR=20.3 (95% CI 4.78-83.2) and seizures AOR=1.62 (95%CI 1.56-4.73).Conclusion : This study is novel as it identifies a possible causal relationship between S. haematobium infection and top morbidity conditions in children under five years. There is need to alert policy makers so as to initiate early treatment of schistosomiasis in pre-school age children.
The chemical composition and manufacturing technology of glass beads excavated from the Hetian Bizili site, Xinjiang
Authors: Wang Dong , Wen Rui , Hu Xingjun , Li Wenying 
Published: March 2020

Abstract
The Hetian Bizili site of the Lop County, located in the southern route of the Silk Road in the Xinjiang, China, was a trade and cultural hub between the East and the West in ancient times. In 2016, a large number of glass beads were unearthed from the 40 tombs excavated in this site. This study analyzed the chemical composition and manufacturing technology of twelve glass beads from the M5 tomb of the Bizili site by using various analytical techniques such as LA-ICP-AES, EDXRF, Raman Spectrometry, and SR-μCT. The chemical compositions of the beads were all Na 2 O-CaO-SiO 2 , with plant ash and natron as fluxes. The lead antimonite and lead stannate were applied as the opacifying agents. Some of the beads with high contents of aluminum may potentially come from Ancient India. In terms of manufacturing technology, the craftsmen made eyeballs of glass beads in different ways, and even applied the same process as Etched Carnelian beads in some beads. This study confirmed that Bizili was an essential place for the interactions between the East and the West and provided the foundation for the spreading of glass beads.
Moral neutralization: Nurses' evolution in unethical climate workplaces
Authors: Hakimi Hamideh , Joolaee Soodabeh , Farahani Mansoureh Ashghali , Rodney Patricia , Ranjbar Hadi 
Published: March 2020

Abstract
Introduction Good quality of care is dependent on nurses’ strong clinical skills and strong moral competencies, as well. However, the moral performance of some nurses in some organizations shows a deterioration in their moral sensitivity and actions. The aim of the study reported on in this paper was to explore the experiences of nurses regarding negative changes in their moral practice. Materials and Methods This was a qualitative study utilizing a inductive thematic analysis approach, which was conducted from February 2017 to September 2019. Twenty-five nurses participated in semi-structured interviews. Results The main theme that emerged from our analysis was one of moral neutralization in the context of an unethical moral climate. We found five sub-themes, including: (1) feeling discouraged; (2) normalization; (3) giving up; (4) becoming a justifier; and (5) moral indifference. Conclusions Unethical moral climates in health organizations can result in deterioration of morality in nurses which can harm both pateints and health syetms. Some unethical behaviors in nurses can be explaiened by this process.
Genetic diagnostic features after failure of initial treatment with epidermal growth factor receptor (EGFR)-tyrosine kinase inhibitors among non-small-cell lung cancer patients harboring EGFR mutations
Authors: Takeda Yuichiro , Naka Go , Yamaguchi Yoh , Hashimoto Masao , Suzuki Manabu , Izumi Shinyu , Sugiyama Haruhito 
Published: March 2020

Abstract
Background: Osimertinib, a third-generation epidermal growth factor receptor (EGFR)-tyrosine kinase inhibitor (TKI), can be used as second-line treatment for lung cancer patients harboring the T790M substitution. Although osimertinib is more effective than first-generation EGFR-TKIs used for first-line treatment, their efficacy for long-term patient survival remains unclear even upon administration of a complete sequence of EGFR-TKI therapy, and limited information is available regrading genetic diagnostic approaches after EGFR-TKI naïve treatment. This study aimed to investigate the characteristics of EGFR-mutated lung cancer patients harboring the T790M substitution resistant to EGFR-TKIs and the advantages of rebiopsy and liquid biopsy among these patients.Methods: The medical records of patients screened for EGFR mutations were reviewed. Upon failure of naïve treatment with EGFR-TKIs except for osimertinib, single plexus COBAS version 2 was repeatedly used to detect the T790M substitution in EGFR via tissue or liquid biopsy. Results: From April 2016 through May 2019, 113 patients harbored EGFR mutations. Sixty patients were treated with EGFR-TKIs, among which 46 underwent tissue or liquid biopsy. Twenty-nine of these 46 (63%) patients harbored the T790M substitution. In total, 141 rebiopsies were performed. The T790M substitution was detected in 24 of 43 tissue and 11 of 98 liquid biopsies. If patients displayed an EGFR exon 19 deletion, displayed a new lesion, and were administered gefitinib as first-line therapy, patients harboring an EGFR mutation were suspected to harbor the T790M substitution. Furthermore, the T790M substitution was detected through rebiopsy in patients with co-existing original mutations, brain metastases, tumor enlargement by ≥ 12 mm, or metastases at rare sites. Conclusion: Repeated biopsy can help maximize the detection rate of the T790M substitution. Furthermore, the advantages of repeated tissue or liquid biopsy should be considered among patients with positive T790M factors, and these biopsies can be repeated numerous times.
Fostering Teaching-Learning through Workplace Based Assessment in Postgraduate Chemical Pathology Residency Program Using Virtual Learning Environment
Authors: Jafri Lena , Siddiqui Imran , Khan Aysha Habib , Tariq Muhammed , Effendi Muhammad Umer Naeem , Naseem Azra , Ahmed Sibtain , Ghani Farooq , Alidina Shahnila , Shah Nadir , Majid Hafsa 
Published: March 2020

Abstract
Background The principle of workplace based assessment (WBA) is to assess trainees at work with feedback integrated into the program simultaneously. A student driven WBA model was introduced and perception evaluation of this teaching method was done subsequently by taking feedback from the faculty as well as the postgraduate trainees (PGs) of a residency program. Methods Descriptive mixed method study was conducted. A WBA program was designed for PGs in Chemical Pathology on MOODLE and forms utilized were Case-based discussion (CBD), Direct Observation of Practical Skills (DOPS) and Evaluation of clinical events (ECE). Consented assessors and PGs were trained on WBA through a workshop. Pretest and post-test to assess PGs knowledge before and after WBA were conducted. Every time a WBA form was filled, perception of PGs and assessors towards WBA, time taken to conduct single WBA and feedback were recorded. Faculty and PGs qualitative feedback on perception of WBA was taken via interviews. WBA tools data and qualitative feedback were used to evaluate the acceptability and feasibility of the new tools. Results Six eligible PGs and seventeen assessors participated in this study. A total of 79 CBDs (assessors n=7 and PGs n=6), 12 ECEs (assessors n=6 and PGs n=5), and 20 DOPS (assessors n=6 and PGs n=6) were documented. PGs average pretest score was 55.6%, which was improved to 96.4% in posttest. Overall mean time taken to evaluate PG’s was 12.6±9.9 min and feedback time 9.2±7.4 min. Mean WBA process satisfaction of assessors and PGs on likert scale of 1 to 10 was 8±1 and 8.3±0.8 respectively. Conclusion Both assessors and fellows were satisfied with introduction and implementation of WBA. It gave the fellows opportunity to interact with assessors more often and learn from their rich experience.
circRAE1 promotes colorectal cancer cell migration and invasion through modulating miR-338-3p/TYRO3 axis
Authors: Du Jiabin , Xu Jianhua , Chen Junxing , Liu Weinan , Wang Pengcheng , Ye kai 
Published: March 2020

Abstract
Background: Growing evidences have revealed that long non-coding RNAs (lncRNAs) including circular RNAs (circRNAs) involve in numerous carcinogenesis. However, the roles of circRNAs in the cancer biology of colorectal cancer (CRC) remain vague. Methods: qRT-PCR and western-blot were used to detecte the circRAE1 levels in CRC tissues and CRC cell lines. Cell proliferation, migration and invasion were detected using wound healing assays, and transwell assays. The interaction between circRAE1 and miR-338-3p and TRYO3 was confirmed by dual-luciferase reporter assays. Results: We uncovered that a novel circRNA Hsa_circ_0060967 (also known as circRAE1) was remarkably increased in CRC tissues, and high circRAE1 level was positively associated with advanced tumor stage, lymph node metastasis, and tumor size. Loss-of-function assay indicated that circRAE1 accelerated cell proliferation, migration and invasion. Besides, miR-338-3p , lowly expressed in CRC tissues and CRC cell lines. dual-luciferase reporter assays showed that circRAE1 could sponge miR-338-3p, which targeted TRYO3 in CRC cells. Furthermore, overexpression of circRAE1 could recue the impaired migration and invasion triggered by miR-338-3p mimics or si-TYRO3 in CRC cells and vice versa. Conclusion : We figured out the network of circRAE1, miR-338-3p, and TYRO3 in CRC cells and revealed that increased circRAE1 served as an oncogene through sponging miR-338-3p, resulting in upregulated TYRO3 expression, which suggested that circRAE1 would be a potential therapeutic target and diagnostic marker for CRC treatment.