bluuebunny/crossref_metadata_2025_split huggingface dataset:
Detection of Methylene Tetrahydrofolate Reductase Gene Polymorphism (C677T) In Sudanese Patients with Chronic Myeloid Leukemia
Authors: Osman Negood Abdelhameed , Gassoum Alsadig , Ahmed Sanabel Alhussien , Babiker Nihad Elsadig 
Published: November 2019

Chronic myeloid leukaemia (CML) is a kind of cancer that affects the white blood cells and resort to progress slowly through many years. It’s occur at any age, but is most common in older (60-65 years) of age. This is a cross sectional study aimed to detect MTHFR gene polymorphism (C677T) among Sudanese patients diagnosed with Chronic Myeloid Leukaemia and conducted at the research laboratory of the national center of neurological sciences (NCNS), Khartoum, Sudan.50 patients with Chronic Myeloid Leukemia (CML) diagnosed as BCR-ABL positive by RT-PCR used as a cases and 50 apparently healthy individuals as a control. A 5 ml of blood samples were collected in EDTA anticoagulant container for DNA Extraction and white blood cells count, hemoglobin level and platelets count. Genotyping of the MTHFR was carried out using PCR technique and the SNP (C677T) confirmed by sequencing a subset of samples. The results were analyzed using bioinformatics tools. The results showed; the most affected age group in the patients was 51-60 years followed by 41-50 years which constituted 32% and 30%, respectively. The hematological findings revealed that, the mean of TWBCs was 47.4, HB was 11.9 for patients, 7.2 and 14.1 respectively for control group (P = 0.000). PLT was 313.5 for patients and 287.5 for control group (P = 0.187). MTHFR gene was detected in the all patients (198pb) by the PCR, Sequence results were aligned with the reference sequence of MTHFR gene, the polymorphic C >T was found to be matched with the registered mutation in NCBI data base. This study provides the first evidence for associations of MTHFR gene polymorphism with the risk of chronic myeloid leukemia in Sudanese patients. The C >T genotype of the rs 677 polymorphism in MTHFR gene may have a promoting effect on chronic myeloid leukemia.
Keywords: Chronic myeloid leukaemia (CML), DNA, PCR, RT-PCR, MTHFR
Antioxidant and Anti-Inflammatory Activities of Caralluma Acutangula (Decne.) N.E.Br Extracts, a Medicinal Plant from Burkina Faso
Authors: Dramane Pare , Yhi-pênê N’do Jotham , Adama Hilou 
Published: November 2019

Plants have always played an important role in health care in Africa. The stress, a situation of imbalance between oxidizing and antioxidant systems in favor of the prooxidants is responsible for the installation of several pathologies such as cancers, cardiovascular diseases, diabetes ... The objective of this study was to highlight the presence Secondary metabolites in C. acutangula extract and determine its antioxidant and anti-inflammatory potential.
For the determination of the acute toxicity of the extract, a dose of 2000 mg / kg was administered to the NMRI Mouse. The methods of screening were used to detect secondary metabolites like tannins, steroids and terpen, flavonoids, coumarins. The antioxidant capacity was evaluated in vitro by determining the ability of the extract to inhibit lipid peroxidation, hydrogen peroxide, degradation of deoxyribose. The anti-inflammatory potential was evaluated on lipoxygenase and xanthine oxidase. Acute toxicity evaluated in NMRI mice showed that the ethanolic extract of C. acutangula show no toxicity. Tannins, steroids and terpen, flavonoids, coumarins have been detected in the extracts. C. acutangula showed good activity with an inhibition of 50.71 ± 2.51% at 100 μg / ml on lipid peroxidation, of 66.105 ± 1.26% on deoxyribose degradation and 8.625 ± 1.09% on hydrogen peroxide. It showed good activity on xanthine oxidase with an 81.5 ± 5.5% inhibition. For the effect on lipoxygenase it gave an inhibition of the enzyme at 43.11 ± 3.4%. This potential could be used in the fight against inflammatory diseases and that due to oxidative stress.
Keywords: antioxidant, anti-inflammatory, oxidative stress, lipid peroxidation
HPTLC method development and validation of stigmasterol from different extracts of Tagetes erecta and Capsicum annuum
Authors: Lal Mohal , Kadam Aboli , Padole Aishwarya S. 
Published: November 2019

A new, simple, sensitive, selective, precise and robust high-performance thin-layer chromatographic (HPTLC) method for analysis of stigmasterol was developed and validated for the determination of stigmasterol in different extracts. A new, simple, sensitive, selective, precise and robust high-performance thin-layer chromatographic (HPTLC) method for analysis of stigmasterol was developed and validated for the determination of stigmasterol in different extracts. Analysis of stigmasterol was performed on TLC aluminium plates pre-coated with silica gel 60F-254 as the stationary phase. Linear ascending development was carried out in twin trough glass chamber saturated with mobile phase consisting of Hexane : Acetone (8:2 v/v) at room temperature. (25 °C ± 2 °C) Camag TLC scanner III was used for spectrodensitometric scanning and analysis in absorbance mode at 490nm. The system was found to give compact spots for stigmasterol. (Rf value of 0.44 ± 0.02) The linear regression analysis data for the calibration plots showed good linear relationship with r2 =0.9997 ± 0.0002 in the concentration range 200-1200 ng spot−1 with respect to peak area. According to the International Conference on Harmonization (ICH) guidelines the method was validated for precision, recovery and robustness. Statistical analysis of the data showed that the method is reproducible and selective for the estimation of stigmasterol.
Keywords: Tagetes erecta, Capsicum annuum, stigmasterol, HPTLC, method validation
Visit-to-Visit HbA1c Variability Is Associated With Cardiovascular Disease and Microvascular Complications in Patients With Newly Diagnosed Type 2 Diabetes
Authors: Li Sheyu , Nemeth Imola , Donnelly Louise , Hapca Simona , Zhou Kaixin , Pearson Ewan R. 
Published: November 2019

OBJECTIVE
To investigate the association between visit-to-visit HbA1c variability and cardiovascular events and microvascular complications in patients with newly diagnosed type 2 diabetes.

RESEARCH DESIGN AND METHODS
This retrospective cohort study analyzed patients from Tayside and Fife in the Scottish Care Information–Diabetes Collaboration (SCI-DC) who were observable from the diagnosis of diabetes and had at least five HbA1c measurements before the outcomes were evaluated. We used the previously reported HbA1c variability score (HVS), calculated as the percentage of the number of changes in HbA1c &gt;0.5% (5.5 mmol/mol) among all HbA1c measurements within an individual. The association between HVS and 10 outcomes was assessed using Cox proportional hazards models.

RESULTS
We included 13,111–19,883 patients in the analyses of each outcome. The patients with HVS &gt;60% were associated with elevated risks of all outcomes compared with the lowest quintile (for example, HVS &gt;80 to ≤100 vs. HVS ≥0 to ≤20, hazard ratio 2.38 [95% CI 1.61–3.53] for major adverse cardiovascular events, 2.4 [1.72–3.33] for all-cause mortality, 2.4 [1.13–5.11] for atherosclerotic cardiovascular death, 2.63 [1.81–3.84] for coronary artery disease, 2.04 [1.12–3.73] for ischemic stroke, 3.23 [1.76–5.93] for heart failure, 7.4 [3.84–14.27] for diabetic retinopathy, 3.07 [2.23–4.22] for diabetic peripheral neuropathy, 5.24 [2.61–10.49] for diabetic foot ulcer, and 3.49 [2.47–4.95] for new-onset chronic kidney disease). Four sensitivity analyses, including adjustment for time-weighted average HbA1c, confirmed the robustness of the results.

CONCLUSIONS
Our study shows that higher HbA1c variability is associated with increased risks of all-cause mortality, cardiovascular events, and microvascular complications of diabetes independently of high HbA1c.
Plasma and Dietary Linoleic Acid and 3-Year Risk of Type 2 Diabetes After Myocardial Infarction: A Prospective Analysis in the Alpha Omega Cohort
Authors: Pertiwi Kamalita , Wanders Anne J. , Harbers Marjolein C. , Küpers Leanne K. , Soedamah-Muthu Sabita S. , de Goede Janette , Zock Peter L. , Geleijnse Johanna M. 
Published: November 2019

OBJECTIVETo study plasma and dietary linoleic acid (LA) in relation to type 2 diabetes risk in post–myocardial infarction (MI) patients.RESEARCH DESIGN AND METHODSWe included 3,257 patients aged 60–80 years (80% male) with a median time since MI of 3.5 years from the Alpha Omega Cohort and who were initially free of type 2 diabetes. At baseline (2002–2006), plasma LA was measured in cholesteryl esters, and dietary LA was estimated with a 203-item food-frequency questionnaire. Incident type 2 diabetes was ascertained through self-reported physician diagnosis and medication use. Hazard ratios (with 95% CIs) were calculated by Cox regressions, in which dietary LA isocalorically replaced the sum of saturated (SFA) and trans fatty acids (TFA).RESULTSMean ± SD circulating and dietary LA was 50.1 ± 4.9% and 5.9 ± 2.1% energy, respectively. Plasma and dietary LA were weakly correlated (Spearman r = 0.13, P &lt; 0.001). During a median follow-up of 41 months, 171 patients developed type 2 diabetes. Plasma LA was inversely associated with type 2 diabetes risk (quintile [Q]5 vs. Q1: 0.44 [0.26, 0.75]; per 5%: 0.73 [0.62, 0.86]). Substitution of dietary LA for SFA+TFA showed no association with type 2 diabetes risk (Q5 vs. Q1: 0.78 [0.36, 1.72]; per 5% energy: 1.18 [0.59, 2.35]). Adjustment for markers of de novo lipogenesis attenuated plasma LA associations.CONCLUSIONSIn our cohort of post-MI patients, plasma LA was inversely related to type 2 diabetes risk, whereas dietary LA was not related. Further research is needed to assess whether plasma LA indicates metabolic state rather than dietary LA in these patients.
Novel Biomarkers for Change in Renal Function in People With Dysglycemia
Authors: Gerstein Hertzel C. , Paré Guillaume , McQueen Matthew J. , Lee Shun Fu , Bangdiwala Shrikant I. , Kannt Aimo , Hess Sibylle 
Published: November 2019

OBJECTIVE
Diabetes is a major risk factor for renal function decline and failure. The availability of multiplex panels of biochemical markers provides the opportunity to identify novel biomarkers that can better predict changes in renal function than routinely available clinical markers.

RESEARCH DESIGN AND METHODS
The concentration of 239 biochemical markers was measured in stored serum from participants in the biomarker substudy of Outcome Reduction With Initial Glargine Intervention (ORIGIN) trial. Repeated-measures mixed-effects models were used to compute the annual change in eGFR (measured as mL/min/1.73 m2/year) for the 7,482 participants with a recorded baseline and follow-up eGFR. Linear regression models using forward selection were used to identify the independent biomarker determinants of the annual change in eGFR after accounting for baseline HbA1c, baseline eGFR, and routinely measured clinical risk factors. The incidence of the composite renal outcome (i.e., renal replacement therapy, renal death, renal failure, albuminuria progression, doubling of serum creatinine) and death within each fourth of change in eGFR predicted from these models was also estimated.

RESULTS
During 6.2 years of median follow-up, the median annual change in eGFR was −0.18 mL/min/1.73 m2/year. Fifteen biomarkers independently predicted eGFR decline after accounting for cardiovascular risk factors, as did 12 of these plus 1 additional biomarker after accounting for renal risk factors. Every 0.1 mL/min/1.73 m2 predicted annual fall in eGFR predicted a 13% (95% CI 12, 14%) higher mortality.

CONCLUSIONS
Adding up to 16 biomarkers to routinely measured clinical risk factors improves the prediction of annual change in eGFR in people with dysglycemia.
Analgesic effects of intraorbital insertion of an absorbable gelatin hemostatic sponge soaked with 1% ropivacaine solution following enucleation in dogs
Authors: Shilo-Benjamini Yael , Slav Shira A. , Kahane Nili , Kushnir Yishai , Sarfaty Havi , Ofri Ron 
Published: November 2019

Abstract

OBJECTIVE
To evaluate analgesic effects and complications associated with intraorbital insertion of an absorbable gelatin hemostatic sponge (AGHS) soaked with 1% ropivacaine solution following enucleation in dogs.

ANIMALS
20 client-owned dogs undergoing enucleation.

PROCEDURES
Dogs were randomly assigned to receive an AGHS soaked with 1% ropivacaine solution (n = 10) or saline (0.9% NaCl) solution (control group; 10) inserted intraorbitally prior to skin closure following enucleation. Carprofen (2 mg/kg [0.9 mg/lb]) was administered SC once after orotracheal extubation and then PO twice a day for 5 days. During the postoperative recovery period, apparent pain level was scored at various points with a modified short-form Glasgow Composite Pain Scale (score range, 0 to 19), and methadone was administered for rescue analgesia if any score was ≥ 5. After dogs returned home, owners recorded their behavior and apparent pain level for the first 3 days following enucleation.

RESULTS
At extubation, the median (range) pain score was significantly higher in the control group (8 [2 to 14]) versus the ropivacaine group (3 [1 to 7]). A greater proportion of dogs in the control group received methadone (7/10 vs 1/10) and had crying or attention-seeking behavior on the first day following enucleation (7/10 vs 1/10). No complications were observed in either group.

CONCLUSIONS AND CLINICAL RELEVANCE
Addition of intraorbital insertion of a ropivacaine-soaked AGHS to the analgesic protocol for dogs undergoing enucleation provided better analgesia than was achieved without this treatment as measured immediately and the first day after surgery, with no noted adverse effects.
Demographics, measures of professional achievement, and gender differences for diplomates of the American College of Veterinary Surgeons in 2015
Authors: Morello Samantha L. , Colopy Sara A. , Bruckner Krista , Buhr Kevin A. 
Published: November 2019

Abstract

OBJECTIVE
To characterize elements of employment, professional success, and personal life for American College of Veterinary Surgeons (ACVS) diplomates and identify elements of practice that may serve as barriers to work-life balance or affect men and women differently.

SAMPLE
836 ACVS diplomates.

PROCEDURES
An 81-item questionnaire was sent to 1,450 ACVS diplomates in 2015 via email and conducted by means of an online platform. Responses were analyzed to identify associations among selected variables.

RESULTS
The survey response rate was 58% (836/1,450). The median age category among respondents was 41 to 45 years. The ratio of male to female diplomates was equivalent among those < 40 years old. Respondents in small animal private practice worked the fewest number of hours; those in equine or large animal private practice worked the most and had the most on-call responsibility. Women were more likely than men to be employed in academia. In both private practice and academia, respondents in small animal practice earned more than did those in equine or large animal practice, and women earned less than did men, even after adjustment for relevant covariates. Women were less likely than men to be practice owners or to hold a prestigious academic title and rank. Perceptions about the effect of gender in the workplace differed between men and women.

CONCLUSIONS AND CLINICAL RELEVANCE
Findings suggested that the veterinary surgical profession is demanding for both genders, although increased flexibility in certain areas may improve work-life balance.
The intersection of personal and professional lives for male and female diplomates of the American College of Veterinary Surgeons in 2015
Authors: Colopy Sara A. , Buhr Kevin A. , Bruckner Krista , Morello Samantha L. 
Published: November 2019

Abstract

OBJECTIVE
To characterize objective and subjective elements of the personal lives of American College of Veterinary Surgeons (ACVS) diplomates and identify elements of personal life associated with professional life or career success.

SAMPLE
836 ACVS diplomates.

PROCEDURES
An 81-item questionnaire was sent to 1,450 diplomates in 2015 via email and conducted by means of an online platform. Responses were analyzed to summarize trends and identify associations among selected variables.

RESULTS
Men were more likely than women to be married or in a domestic partnership (88% vs 68%, respectively) and to have children (77% vs 47%). Among women but not men, respondents in large animal practice were less likely than were those in small animal practice to be represented in these categories. Women had children later in their career than did men and indicated that their stage of training played an important role in family planning. Respondents with children worked significantly fewer hours than did those without children, with a greater reduction in hours for women versus men (6.0 vs 3.1 hours, respectively). Women were more likely to require external childcare services than were men. Women were more likely to report that having children had negatively impacted their professional lives. No negative associations between measures of professional success (eg, advancement or personal income) and parenthood were identified.

CONCLUSIONS AND CLINICAL RELEVANCE
Family demographics differed between male and female ACVS diplomates, yet no objective impact on career success was identified. Work-life balance may play an important role in recruitment, retention, and job satisfaction of veterinary surgeons.
İğde (Elaeagnus angustifolia L.)’nin Yetiştiği Toprakların Bazı Fiziksel ve Kimyasal Özellikleri ile Ağır Metal Durumunun Değerlendirilmesi: Erzurum Örneği
Authors: YILDIZ Nesrin , ZENGİN Murat 
Published: November 2019

Bu çalışmasının amacı Erzurum kentinde iğde bitkisinin (Elaeagnus angustifolia L.) yetiştiği 4
lokasyon (Şehir Merkezi, Üniversite, Yenişehir/Yıldızkent yerleşim alanı ve
Dadaşkent yerleşim alanı)’da 5 farklı noktadan alınan toprak örneklerinin bazı
kimyasal ve fiziksel özellikleri (toprak tekstürü, pH, CaCO3,
organik madde, KDK, değişebilir K) ile ekstrakte edilebilir potansiyel toksik
ağır metal (Pb, Co, Cd ve Ni) içeriklerini analiz etmektir. Toprak
örneklerindeki ağır metal analizleri Atomik Absorbsiyon Spektrofotometre
cihazında yapılmıştır. Ölçüm değerlerinin istatistiksel açıdan anlamlılığı
Duncan Testi ile belirlenmiştir. Elde edilen verilerin ışığında lokasyonlarda
toprakların pH bakımından nötr ile hafif alkali olduğu, kireçlilik bakımından
kireçli ve orta kireçli, organik madde içeriği bakımından iyi düzeyde olduğu,
katyon değişimi bakımından mevcut kil içeriği ile uyumlu olduğu, değişebilir
potasyum açısından fazla ve çok fazla sınıfına girdiği belirlenmiştir. Toprak
tekstürü bakımından ise Yenişehir/Yıldızkent toprak örnekleri tınlı iken diğer
istasyonlar kumlu tınlı olduğu tespit edilmiştir. Ağır metal değerlerinin izin
verilebilir sınır değerler ile karşılaştırıldığında ağır metal
içeriklerinin (Pb, Co, Cd ve Ni) kritik
değerlerin altında olduğu belirlenmiştir. Lokasyonlara göre topraktaki ağır
metal konsantrasyonlarının “ppb” düzeyinde düşük sınırlarda olduğu ve
istasyonlardaki topraklarda ağır metallerin (Pb, Co, Cd ve Ni) toksisite riski
bulunmadığı belirlenmiştir.
ANALISIS KEBUTUHAN PENGEMBANGAN SDM UNTUK MENINGKATKAN KINERJA PEGAWAI DALAM DI BKP KELAS I MATARAM
Authors: Agussaleh Agussaleh , Agusdin Agusdin , Hermanto Hermanto 
Published: November 2019

Tujuan penelitian ini adalah untuk Analisis Kebutuhan Pengembangan SDM untuk Meningkatkan Kinerja Pegawai dalam Implementasi SMM ISO 9001:2015 di BKP Kelas I Mataram. Penelitian ini adalah penelitian kualitatif, Kriteria informan yang digunakan dalam penelitian ini adalah seseorang yang memiliki kewenangan dalam mengatur, mengawasi, mengaudit serta pelaksana yang terkait langsung dengan keberlangsungan implementasi SMM ISO 9001 : 2015 di BKP Mataram. Analisa data kualitatif terdiri dari tiga alur kegiatan yang dilakukan secara bersamaan yaitu reduksi data, penyajian data dan penarikan kesimpulan.Hasil penelitian menunjukan bahwa BKP Kelas I Mataram telah melakukan pengembangan SDM dan menerapkan SMM ISO 9001:2015 untuk meningkakan kinerja pegawai dan organisasi, upaya peningkatan kinerja yang dilakukan mulai : 1). pengembangan organisasi (OD) berupa penerapan system kerja melalui implementasi Standart Manajemen Mutu melaui ISO 9001:2015, SNI ISO 17025 akreditasi llaboratorium dan ISO 37001 tentang sistem manajemen anti penyuapan; 2) Pengembangan Non Aparatur (N-ED) melalui Sosialisasi karantina; 3) Pengembangan Aparatur (ED) melaui peningkatan jenjang pendidikan ke jenjang yang lebih tinggi; 4) Pendidikan Aparatur (E-Ed) melalui pelatihan dasar karantina dan Training Centre; 5) Pelatihan Aparatur (ET) berupa kegiatan magang pe, inhouse training, workshop dan pembinaan mental pegawai.
Comprehensive Polyphenolic Profile of Plantago depressa using High‐Speed Countercurrent Chromatography Off‐line with High‐Performance Liquid Chromatography–Diode Array Detector–Quadrupole Time‐of‐flight Tandem Mass Spectrometry
Authors: Xu Jinju , Tong Chaoying , Fu Qiachi , Guo Keke , Shi Shuyun , Xiao Yecheng 
Published: November 2019

The whole plant of Plantago depressa is commonly used as traditional Chinese medicine and functional food ingredient. Total phenolic content and total flavonoid content evaluation indicated that its ethyl acetate and n‐butanol fractions were rich in polyphenols. However, to date, the polyphenolic profile is limited because of the complex matrix. Here, polyphenols in ethyl acetate and n‐butanol fractions of P. depressa were first separated and enriched by High‐Speed Countercurrent Chromatography (HSCCC). Then, their structures were characterized or tentatively identified by High‐Performance Liquid Chromatography (HPLC)–Diode Array Detector (DAD)–Quadrupole Time‐of‐Flight Tandem Mass Spectrometry (QTOF‐MS/MS) (HSCCC × HPLC–DAD–QTOF‐MS/MS). Because of the orthogonality of HSCCC and HPLC, 37 polyphenols were identified. Notably, 29 polyphenols were discovered from P. depressa for the first time. Finally, contents of two potential new compounds, 2‐ethoxy plantamajoside isomers (17 and 29), were determined as 0.27 ± 0.03 and 0.04 ± 0.01 mg/g, respectively. Results indicated that P. depressa could be used as a promising source of polyphenols for health promotion, and the developed method (HSCCC × HPLC) was efficient to explore polyphenolic profile for complex natural products.
BOS Kültür Antibiyogram Sonuçları ve Olası Enfeksiyon Nedenleri
Authors: ÖZTÜRK Ünal , ASENA Muhammet , AYDIN ÖZTÜRK Pinar 
Published: November 2019

Amaç: Merkezi sinir sistemi infeksiyonları tüm dünyada morbidite ve
mortaliteye neden olan ciddi enfeksiyonlar olup erken tanı, erken ve uygun
antibiyotik tedavisi ile mortalitesi ve morbiditesi azaltılabilen bir hastalık
grubudur. Ampirik antibiyotik tedavisi beyin omurilik sıvısının (BOS)
mikrobiyolojik inceleme sonuçlarına göre başlanmalı, kültür antibiyogram
sonuçlarına göre düzenlenmelidir. Hastanelerdeki mikroorganizmaların direnç
profilinin belirli aralıklarla gözden geçirilmesi etkin tedavinin erken başlamasını
sağlayabilecektir.

Yöntem: Çalışma için 167 BOS kültür antibiyogram sonucu değerlendirildi. BOS
kültürü alınan hastalar retrospektif olarak değerlendirilip hastaların yaşı,
cinsiyeti, tanısı, uyruğu, yattığı veya başvurduğu birim, kültür örneğinin
alındığı tarih ve kültür sonuçları kaydedildi.

Bulgular: Kültür sonuçlarının %19,6’sında üreme varken, %81,4’ünde üreme
olmadı. Kültürde üreme saptanan hastaların %41.9’nda gram negatif
mikroorganizma, %51,6’sında gram pozitif mikroorganizma ve %6,4’nde mantar
üremesi saptandı. En sık üreyen etken koagulaz negative stafilokoklar iken en
sık BOS kültüründe üreme nedeni şant enfeksiyonları olarak belirlendi.

Sonuç: Çalışmamızda üreyen mikroorganizmaların çoğu sağlık bakım ilişkili
enfeksiyon etkenleri olup antibiyotik direncinin yüksek olduğu, yoğun bakım ve
şant kaynaklı enfeksiyonların daha uzun süre tedavi gerektirdiği
görülmüştür.