bluuebunny/crossref_metadata_2025_split huggingface dataset:
Inhibition of Selenoprotein I promotes ferroptosis and reverses resistance to platinum chemotherapy by impairing Akt phosphorylation in ovarian cancer
Authors: Li Jing , Chen Mimi , Huang Dingwen , Li Ziyin , Chen Yu , Huang Jinhua , Chen Yuanqun , Zhou Zhili , Yu Zhiying 
Published: December 2024

AbstractOvarian cancer (OV) ranks among the deadliest gynecological cancer, known for its high risk of relapse and metastasis, and a general resistance to conventional platinum‐based chemotherapy. Selenoprotein I (SELENOI) is a crucial mediator implicated in human hereditary spastic paraplegia. However, its role in human tumors remains poorly elucidated. Here, we comprehensively analyzed SELENOI expression patterns, functions, and clinical implications across various malignancies through the integration of bulk transcriptomics, cancer databases, and in vitro and in vivo experiments. Pan‐cancer analysis indicated upregulated SELENOI expression across various cancers, correlating with augmented malignancy, suppressed tumor immunity and poor prognosis. Knockdown of SELENOI caused G0/G1‐phase cell cycle arrest and diminished aggressive cancer phenotypes in OV cells. Moreover, SELENOI inhibition augments ferroptosis and reverses the cisplatin resistance in OV cells by modulating Akt phosphorylation. Conversely, overexpression of SELENOI in OV cells enhanced therapeutic sensitivity to cisplatin by upregulating Akt phosphorylation. Importantly, in vivo studies demonstrated that SELENOI inhibition suppressed ovarian tumor growth and enhanced cisplatin's anticancer effects. These findings highlight the significant role of SELENOI in OV by modulating ferroptosis and chemotherapy resistance. Targeting SELENOI represents a promising therapeutic approach to promote the efficacy of platinum‐based chemotherapy in OV, particularly in cases of resistance.
Service Guarantee Decision of Online Service Providers and Market Implications
Authors: Lu Aijun , Chen Fuzan , Wu Harris 
Published: December 2024

ABSTRACTOnline services face quality uncertainties. To address user concerns, Online Service Providers (OSPs) sometimes provide service guarantees, compensating users if quality falls below a predefined threshold. This paper analyzes how OSPs use service guarantees to navigate market competition and enhance revenue through game theory and queuing theory models. Key findings include the following: offering guarantees enable OSPs to increase prices; in monopolistic markets, guarantees are offered only if the service guarantee strength is sufficient, whereas in competitive markets, decisions depend on relative strengths and competitor actions. Surprisingly, slower response times under service guarantees can boost profits, especially when resource costs are high.
Electrical Interference in Clinical Practice: A Conceptual and Practical Approach
Authors: Dumitru Daniel , Barkhaus Paul E. , Nandedkar Sanjeev D. 
Published: December 2024

ABSTRACTElectrical Interference (EI: radiated electromagnetic and/or power line interference) is a common problem in clinical neurophysiology with many causes and thus various conceivable solutions. Although newer digitized electrodiagnostic (EDX) systems have markedly reduced EI issues, it remains a possible impediment in achieving high quality studies. So that the electrodiagnostic medicine consultant (EMC) can problem solve EI, this monograph details the fundamental functional concepts and terminology of electronic amplification and recording electrodes from a practical perspective. This information is then utilized in a proposed standard operating protocol (SOP) to help the EMC address a wide variety of EI sources. Three major EI sources are considered: the EDX system/operator error, the environment, and the patient. The first is a thorough assessment of the recording electrodes from the perspective of clean electrodes, security of attachment, appropriate gel application, proper lead connections to both the patient and instrument, and similarity of electrode composition. Second is how adverse environmental conditions are mitigated through isolating the EDX instrument from nearby large generator sources, unplugging unnecessary equipment, keeping the amplifier close to the patient along with short and braided electrode leads, and utilize filtering (both 60 Hz and total bandwidth) with appropriate caution to avoid unwanted signal distortion. Third, the patient and EMC interaction must be considered. Specifically, all electronic devices that can be removed should be powered down and relocated as far as feasible from the EDX system, including digital watches, cell phones, TENS units, and other such devices. A systematic application of the above proposed protocol should solve the majority of EI issues.
Sequential Maximal Updated Density Parameter Estimation for Dynamical Systems With Parameter Drift
Authors: del‐Castillo‐Negrete Carlos , Spence Rylan , Butler Troy , Dawson Clint 
Published: December 2024

ABSTRACTWe present a novel method for generating sequential parameter estimates and quantifying epistemic uncertainty in dynamical systems within a data‐consistent (DC) framework. The DC framework differs from traditional Bayesian approaches due to the incorporation of the push‐forward of an initial density, which performs selective regularization in parameter directions not informed by the data in the resulting updated density. This extends a previous study that included the linear Gaussian theory within the DC framework and introduced the maximal updated density (MUD) estimate as an alternative to both least squares and maximum a posterior (MAP) estimates. In this work, we introduce algorithms for operational settings of MUD estimation in real‐ or near‐real time where spatio‐temporal datasets arrive in packets to provide updated estimates of parameters and identify potential parameter drift. Computational diagnostics within the DC framework prove critical for evaluating (1) the quality of the DC update and MUD estimate and (2) the detection of parameter value drift. The algorithms are applied to estimate (1) wind drag parameters in a high‐fidelity storm surge model, (2) thermal diffusivity field for a heat conductivity problem, and (3) changing infection and incubation rates of an epidemiological model.
Electromagnetic shielding property and electrical conductivity of multi‐walled carbon nanotubes filled poly(ε‐caprolactone) composites with dense conductive networks: A simulation and experimental study
Authors: Zheng Yu‐Chen , Jiang Ke , Hou Xin , Feng Xue‐Rong , Liu Jiang‐Tao , Wang Ming 
Published: December 2024

AbstractElectromagnetic shielding properties of conductive polymer composites (CPCs) are dependent on the content of conductive fillers. In this study, electromagnetic shielding effectiveness (EMW SE) of poly(ε‐caprolactone) (PCL) filled with different content of multi‐walled carbon nanotubes (MWCNTs) were prepared to systematically discuss content‐dependent electromagnetic shielding mechanism via a simulation and experimental study. At low MWCNT loadings, the composites have high absorption coefficients because the MWCNT particles are separated by PCL chains. The shielding performance mainly comes from the polarization loss and conduction loss. However, the composites with high content of MWCNT can form conductive networks which leads to high reflection values, suggesting a reflection‐dominant shielding mechanism. In addition, a conductive mesh model is first introduced to simulate the effect of filler loadings on electromagnetic shielding performance. For electrical conductivity, there is no obvious platform being found in the samples at a high content of MWCNTs. The formation of a denser conductive network within the composites reduces inter‐filler gaps and promotes closer contact. However, according to the experimental and simulation results, upon exceeding the percolation threshold of MWCNT loadings, the EM SE value slightly increases leading to a plateau region. The results are ascribed to the formation of stable shielding networks at high content of MWCNTs.Highlights
The sample has a high absorption coefficient at a low content of MWCNTs.
All the samples exhibit a reflection‐dominant shielding mechanism.
No obvious platform of conductivity is found at high content of MWCNTs.
Obvious platform of EMW SE values is found in the high content of MWCNTs.
High EMW SE values for CPC only require relatively dense conductive networks.
A Unique Ceruloplasmin Shows Distinct Redox Potentials for the Catalytic and Electron‐Transfer Metal Sites
Authors: Yadav Pramod K. , Barman Soumen , Rajbongshi Jitumani , Mishra Rajeev , Mhaske Sanjay , Sudhamalla Babu , Mazumdar Shyamalava 
Published: December 2024

ABSTRACTCeruloplasmin, a multicopper oxidase essential for copper metabolism and oxidative defense, from blood serum of domestic goats (Capra aegagrus hircus) has been purified to homogeneity by using three steps of column chromatography. The purified goat ceruloplasmin (gCP) protein has an estimated molecular weight of approximately 141 kDa as determined by SDS‐PAGE. ICP‐AES analysis indicates that each molecule of the protein contains 5.6 ± 0.2 atoms of copper. The optimum pH of catalysis for gCP was found to be pH 5.5. Using the Hill equation, the Km and Vmax values for gCP are estimated to be 0.41 ± 0.01 mM and 0.35 ± 0.01 μmol/min/mg, respectively. The enzyme has an average of 3.3 ± 0.33 substrate binding sites per molecule. Both, circular dichroism (CD) spectroscopy studies and computational analysis using PDBsum reveals that gCP is primarily composed of β‐strands distinguishing it from the α‐helix‐rich human ceruloplasmin. Also, computational analysis of gCP reveals six copper‐binding sites, composed of histidine (his), methionine (Met), and cysteine (cys). Cyclic voltammetry studies of gCP immobilized on a glassy carbon electrode indicate the presence of Type 1 and Type 2/3 copper centers within the protein identified by two unique redox potential values of +294 mV and −250 mV versus the normal hydrogen electrode (NHE), respectively. The positive redox potential site that probably acts as the electron‐transfer center had a slightly lower redox potential compared to that reported in other ceruloplasmin. The negative redox potential site of the protein possibly corresponds to the catalytic site containing trinuclear copper center proposed earlier. The redox potential of the Type 1 center aligns closely with azurin, suggesting evolutionary homology and suitability for direct electron transfer (DET). The distinct electrochemical properties of gCP may enhance applications in biofuel cells, biosensors, and other bioelectronic devices due to its efficient electron‐transfer capabilities. This study provides valuable insights into the functional versatility of ceruloplasmin across species and highlights the biotechnological potential of this unique variant.
In Silico Optimization of a Streptomyces sp. Antimicrobial Peptide Against Oncobacteria in Oral Squamous Cell Carcinoma
Authors: Taj Zarin , Marisamy Vannamathi , Chattopadhyay Indranil 
Published: December 2024

ABSTRACTOral squamous cell carcinoma (OSCC) is a major public health concern, accounting for more than 90% of all oral malignancies. Pathogenic bacteria, such as Streptococcus mutans, Fusobacterium nucleatum, and Porphyromonas gingivalis, are closely linked to OSCC and promote chronic inflammation, a key factor in the tumor microenvironment. The increasing incidence of oral malignancies necessitates the development of antimicrobial peptides (AMPs) with enhanced anticancer activity as targeted therapeutics against bacteria to mitigate their impact on OSCC. A total of 182 Streptomyces genus‐derived AMPs were collected and analyzed in silico to determine their potent antibacterial and anticancer characteristics. Fourteen AMPs were tested for 3D structural alignment prediction and validation, and the most efficient peptide was determined to be sAMP2. A genome‐based analysis of the peptide sourced from Streptomyces globisporus C‐1027 was conducted to explore its evolutionary significance. The cell‐penetrating peptide activity of sAMP2 was optimized to make it more stable and better able to target OSCC host cellular receptors. The peptide Mut_sAMP2 was found to interact with the bacterial virulence proteins DEX of S. mutans, LPS of F. nucleatum, and KGP of P. gingivalis and with OSCC‐associated proteins (TNFα, IL‐6, IL‐8, p38, p53, E‐cadherin, Jak‐1, and PAR2) with greater binding affinity. The peptide Mut_sAMP2 treated OECM‐1 cells showed reduced cell survival by the cytotoxicity activity of IC50 5.4 μg/mL. To the best of our knowledge, our computational study is the first to significantly impact the development of new and effective cancer therapeutics from Streptomyces sp. derived AMPs with a broad spectrum of activity and potential evaluating to combat OSCC progression linked with oral oncobacteria.
Integrating management tactics to mitigate Spodoptera frugiperda resistance in Bt corn: refuge proportions, natural parasitoids, and selective insecticide application
Authors: Tomé Maysa P , Weber Igor D , Garcia Adriano G , Godoy Wesley AC 
Published: December 2024

AbstractBACKGROUNDDifferent approaches have been adopted to manage Spodoptera frugiperda resistance to Bt toxins. However, studying the synergism among these practices applied directly in crop fields is a major challenge. We used a computational model to investigate how the proportion of refuge strips [crop area occupied by non‐Bt corn (maize): 5%, 10%, 15%, or 20%] and the presence of naturally occurring parasitoid, affected or not by a pesticide with different selectivities applied in the field, could influence the dynamics of the resistance allele (R) in a S. frugiperda population.RESULTSOur model showed that proportions of 15% and 20% of non‐Bt corn, together with parasitism, led to a reduction in the initial frequency of the R allele. However, the efficiency of the parasitoid depended on the selectivity of the insecticide. Simulations with 5% non‐Bt corn and 50% selectivity, or without selectivity (0%), resulted in elimination of the parasitoid. In the absence of selection pressure, S. frugiperda larvae carrying at least one copy of the R allele showed higher susceptibility to parasitization.CONCLUSIONOur findings suggest that parasitoids can potentially reduce the need for refuge zones, because they offset the reduction of non‐Bt crops. Nevertheless, preservation of these benefical insects hinges upon the implementation of appropriate management practices by producers. Further investigations are warranted to corroborate these results, including the study of additional natural enemies. © 2024 Society of Chemical Industry.
New 5,6‐dihydrobenzo[h]quinoline derivatives as potential demethylase inhibitors (DMIs): design, synthesis, activity evaluation and molecular dynamics simulation
Authors: Xie Xiansong , Wang Jingwen , Bao Ailing , Deng Ziquan , Wang Deyuan , Chen Wenrui , Jiang Wenjing , Li Weiyi , Tang Xiaorong , Yan Yingkun 
Published: December 2024

AbstractBACKGROUNDBipolaris maydis is a serious plant fungus and strongly affects the yield and quality of crops. The main control strategy is the employment of fungicides. To research efficient fungicide with novel structure, a series of novel 5,6‐dihydrobenzo[h]quinoline derivatives were designed and synthesized.RESULTSThirty‐six novel 5,6‐dihydrobenzo[h]quinoline analogues were designed and synthesized. The assay results showed that most compounds exhibited significant fungicidal activity against Pyricularia oryzae, Bipolaris maydis, Sclerotinia sclerotiorum, Penicillium digitatum and Valsa mali at 16 μg mL−1. Compounds 4 h, 5e, 6a and 6b showed better antifungal activity than fluquinconazole against B. maydis. Their half maximal effective concentration (EC50) values were 0.732, 0.283, 0.529, 0.644 and 0.826 μg mL−1, respectively. Furthermore, the bioactive compounds were determined against sterol 14α‐demethylase (CYP51). The results indicated that they displayed prominent inhibiting activities, 4 h, 5e, 6a and 6b also had better inhibitory activities than fluquinconazole against CYP51. Their half maximal inhibitory concentration (IC50) values were 0.840, 0.315, 0.601, 0.750 and 1.018 μg mL−1, respectively. The fluorescent quenching tests of proteins indicated that the quenching patterns of compounds 5e and 6a were analogous to fluquinconazole. The molecular dynamics (MD) simulations indicated that compound 5e possessed stronger affinity than fluquinconazole to CYP51.CONCLUSIONThe results of the present study displayed that novel 5,6‐dihydrobenzo[h]quinoline derivatives could be one scaffold of potential CYP51 inhibitor and will provide some valuable information for the research and development of new fungicides. © 2024 Society of Chemical Industry.
Mobility as Capital: A ‘Three‐Step’ Education Pathway of Chinese Transnational Migrant Families in Zimbabwe
Authors: An Ning , Li Jin L. 
Published: December 2024

ABSTRACTSince the raising of China's ‘Going‐Out’ policy, the number of Chinese residing in Africa has increased dramatically, providing these families with greater convenience in cross‐border mobility. This article empirically examines this emerging transnational population of Chinese families in Zimbabwe, focusing on the social reproduction of this relatively understudied group. We identify an educational migration strategy used by these families as a ‘three‐step’ pathway: sending their children back to China for primary school, returning to Zimbabwe for middle school, and ultimately aiming for admission to Western universities. Using Bourdieu's notion of capital, we analyse how these educational decisions facilitate the transformation of family mobility into cultural and social capital, reinforcing transmigrant family strategies of social reproduction. This study bridges the gap between preuniversity educational mobility and lifelong transnational mobility aspirations, highlighting the foundational role of family influence. It broadens the theoretical scope of international education research by examining how Chinese primary education, Zimbabwean secondary education, and Western higher education, each as an imagined community, collectively shape family aspirations and emphasise the enduring impact of family plans on international student mobility.
Unraveling Quercetin's Potential: A Comprehensive Review of Its Properties and Mechanisms of Action, in Diabetes and Obesity Complications
Authors: Yi Ruhan , Liu Yun , Zhang Xu , Sun Xiance , Wang Ningning , Zhang Cong , Deng Haoyuan , Yao Xiaofeng , Wang Shaopeng , Yang Guang 
Published: December 2024

ABSTRACTThe prevalence of diabetes is escalating alarmingly, placing a significant economic burden on the global healthcare system. The use of chemical substances extracted from plants has been demonstrated to be an effective method for the treatment and control of insulin resistance and Type 2 diabetes mellitus (T2DM). New research indicates that natural phytochemicals present in fruits and vegetables are expected to become drugs for the treatment of diabetes and the prevention of related complications. Quercetin, a widely distributed flavonoid, is well‐known for its antioxidant, anti‐inflammatory, anticancer, and antidiabetic properties. This article provides a comprehensive account of the mechanism of action of quercetin on diabetes and obesity complications in vivo and in vitro. It elucidates the impact of quercetin on various cells. These include hepatocytes, renal cells, skeletal muscle cells, and adipocytes. Furthermore, this article discusses the mechanism of quercetin on organ damage in diabetic mice induced by STZ, alloxan, diet, and spontaneous Type 2 diabetic mice caused by genetic defects. Additionally, it addresses the pharmacokinetics of quercetin and its potential for synergistic effects with existing diabetic drugs.
Current insights and future directions in Peyronie's disease management: A narrative review
Authors: Howell Skyler , Green Travis , Khalafalla Kareim 
Published: December 2024

AbstractPeyronie's disease (PD) is an inflammatory and fibrotic disease which results in disfiguring and often distressing penile curvature deformity, affecting up to one in nine men in the United States, and between 0.3% and 13.1% of men globally. It progresses through an acute phase, associated with pain, as the fibrosis develops. In the quiescent phase, penile pain ceases and deformity stabilizes. The precise etiology remains unknown despite ongoing work to elucidate the biological underpinning. The diagnosis is guided by history and physical examination. Except for ultrasonography, imaging is not routinely recommended. Current management is predicated on symptomatic control and slowing progression in the acute phase, and correction of bothersome curvature in the stable phase. Most nonsurgical treatment options are poorly supported by available evidence, with the exceptions of traction therapy and certain intralesional injections. Surgical treatment, considered only after stabilization, is guided by severity and the presence or absence of erectile function and is highly individualized. Investigations are ongoing into several areas, including the exact biological mechanisms leading to plaque formation and failure of resolution; the effects of co‐existing systemic disease; the role of imaging in diagnosis and surgical planning; combination and regenerative nonsurgical therapies; and improvements in surgical techniques. As diagnostic accuracy improves and targeted treatments become available, management of PD will become progressively tailored to an individual's particular disease. In this review, we summarize the current knowledge regarding PD, including etiology and epidemiology, diagnosis, management, cutting‐edge research, and future directions in care of this condition.
IQWiG-Evidenzbericht zum Thema psychische Folgen eines Schwangerschaftsabbruchs
Authors: Endres David , Gowik Julia , Tasar Andrea 
Published: December 2024

Zusammenfassung
Das Institut für Qualität und Wirtschaftlichkeit im Gesundheitswesen (IQWiG) wurde vom Bundesministerium für Gesundheit beauftragt, bei der Weiterentwicklung der S2k-Leitlinie „Schwangerschaftsabbruch im ersten Trimenon“ zu einer S3-Leitlinie zu unterstützen. Dazu wurden von der zuständigen Leitliniengruppe Fragestellungen formuliert, die in Evidenzberichten des IQWiG beantwortet wurden. Für eine dieser Fragestellungen sollte die Evidenz bezüglich der psychischen Folgen eines Schwangerschaftsabbruchs im ersten Trimenon im Vergleich zu keinem Schwangerschaftsabbruch im ersten Trimenon bei Schwangeren mit dem Wunsch nach einem Schwangerschaftsabbruch dargestellt werden. In einer systematischen Recherche wurde eine relevante prospektiv vergleichende Kohortenstudie identifiziert, die Ergebnisse zu den Endpunkten klinisch diagnostizierte Depressionen und klinisch diagnostizierte Angststörungen berichtete. Die Evidenz wurde auf Grundlage der methodischen Vorgaben von „Grading of Recommendations Assessment, Development and Evaluation“ (GRADE), konkretisiert durch die IQWiG-Methodik, beurteilt. Es konnten keine signifikanten Unterschiede zwischen den Gruppen zu den genannten Endpunkten festgestellt werden. Die Vertrauenswürdigkeit der Evidenz wurde als sehr niedrig eingestuft, Abwertungen ergaben sich durch Studienlimitationen und fehlende Genauigkeit der Effekte. Prospektive vergleichende Kohortenstudien, die sich der untersuchten Fragestellung nähern, sollten unter anderem eine adäquate Kontrolle für relevante Störgrößen, eine ausreichende Anzahl an Teilnehmerinnen sowie eine sorgfältig geplante Erhebung relevanter Endpunkte aufweisen.