bluuebunny/crossref_metadata_2025_split huggingface dataset:
The identification and determination of toxin genes of Vibrio strains causing hemorrhagic disease on red drum (Sciaenops ocellatus) using PCR
Authors: Pham Yen Thi Hai , Linh Nguyen Quang , Nguyen Tram Duy Quynh 
Published: December 2020

Abstract
Data were collected from 30 strains of Vibrio and sampled on different organs (brain, hemorrhagic site and digestive tract) of Sciaenops ocellatus infection. The results showed that the nucleotide sequences 16S rRNA region are highly similar to those of V. alginolyticus, V. azureus, V. fluvialis, V. natriengens and V. orientalis, which were published on Genbank and other, ranging from 98.05 to 100 %. The digestive tract has the most common Vibrio strains (V. alginolyticus [16] V. azureus [7] and V. fluvialis). Thereout, 25 of 30 strains of Vibrio contained 1 to 3 toxin genes, except V. parahaemolyticus. Six parameters were used to measure the DNA polymorphism of 33 homologous DNA sequences in this Vibrio bacteria population. The results indicated that number of separate polymorphic sites (S), total number of mutant sites (Eta), number of haplotype (h), haplotype diversity (Hd), average number of nucleotide differences (k), nucleotide diversity (Pi) were 98 (S), 103 (Eta), 9 (h), 0.887 ± 0.032 (Hd), 25.789 (k) and 17.980x10-3 ± 0.003 (Pi), respectively (P < 0,05). The G+C content above 1434 sites positions of nucleotide sequences accounted for 0.542. The phylogenetic tree showed that these strains are divided into six groups. As observed, the appearance of isolated Vibrio on 3 organs of fish (S. ocellatus) hemorrhagic are V. azureus (27,67 %), V. alginolyticus (50 %), V. orientalis (6,67 %) and V. fluvialis (16,67 %). Through this result, we found that the diversity of Vibrio species that appeared on the red drum was used in the 16S rRNA region and the presence of toxin genes in these Vibrio species.
Willingness of the UK public to volunteer for testing in relation to the COVID-19 pandemic
Authors: Vandrevala Tushna , Montague Amy , Terry Philip , Fielder Mark D. 
Published: December 2020

Abstract
Background: The World Health Organization declared the rapid spread of COVID-19 around the world to be a global public health emergency. The spread of the disease is influenced by people’s willingness to adopt preventative public health behaviours, such as participation in testing programmes and risk perception can be an important determinant of engagement in such behaviours. Methods: In this study, we present the first assessment of how the UK public (N=778) perceive the usefulness of testing for coronavirus and the factors that influence a person’s willingness to test for coronavirus.Results: None of the key demographic characteristics (age, gender, education, disability, vulnerability status, or professional expertise) were significantly related to the respondents’ willingness to be tested for coronavirus. However, closely following the news media was positively related to willingness to be tested. Knowledge and perceptions about Coronavirus significantly predicted willingness to test, with three significantly contributing factors: worry about the health and social impacts to self and family; personal susceptibility; and concerns about the impacts of coronavirus on specific demographic groups. Views on testing for coronavirus predicted willingness to test, with the most influential factors being importance of testing by need; negative views about widespread testing and mistrust in doctor’s advice about testing. Conclusions: Implications for effective risk communication and localised public health approach to encouraging public to put themselves forward for testing are discussed. We strongly advocate for effective communications and localised intervention by public health authorities, using media outlets to ensure that members of the public get tested for SARs-CoV2 when required.
Identification of evolutionarily stable sites across the SARS-CoV-2 proteome
Authors: Wang Chen , Konecki Daniel M. , Marciano David C. , Govindarajan Harikumar , Williams Amanda M. , Wastuwidyaningtyas Brigitta , Bourquard Thomas , Katsonis Panagiotis , Lichtarge Olivier 
Published: December 2020

Abstract
Since the first recognized case of COVID-19, more than 30 million people have been infected worldwide. Despite global efforts in drug and vaccine development to fight the disease, there is currently no vaccine or drug cure for COVID-19, though some drugs reduce severity and hasten recovery. Here we interrogate the evolutionary history of the entire SARS-CoV-2 proteome to identify functional sites that can inform the search for treatments. Combining this information with the mutations observed in the current COVID-19 outbreak, we systematically and comprehensively define evolutionarily stable sites that are useful drug targets. Several experimentally-validated effective drugs interact with these proposed target sites. In addition, the same evolutionary information can prioritize cross reactive antigens that are useful in directing multi-epitope vaccine strategies to illicit broadly neutralizing immune responses to the betacoronavirus family. Although the results are focused on SARS-CoV-2, these approaches are based upon evolutionary principles and are agnostic to organism or infective agent.
Hydrological model optimization using multi-gauge calibration (MGC) in a mountainous region
Authors: Swalih Sead Ahmed , Kahya Ercan 
Published: December 2020

Abstract
It is a challenge for hydrological models to capture complex processes in a basin with limited data when estimating model parameters. This study aims to contribute in this field by assessing the impact of incorporating spatial dimension on the improvement of model calibration. Hence, the main objective of this study was to evaluate the impact of multi-gauge calibration in hydrological model calibration for Ikizdere basin, Black Sea Region in Turkey. In addition, we have incorporated the climate change impact assessment for the study area. Four scenarios were tested for performance assessment of calibration: (1) using downstream flow data (DC), (2) using upstream data (UC), (3) using upstream and downstream data (Multi-Gauge Calibration – MGC), and (4) using upstream and then downstream data (UCDC). The results have shown that using individual gauges for calibration (1 and 2) improve the local predictive capacity of the model. MGC calibration significantly improved the model performance for the whole basin unlike 1 and 2. However, the local gauge calibrations statistical performance, compared to MGC outputs, was better for local areas. The UCDC yields the best model performance and much improved predictive capacity. Regarding the climate change, we did not observe an agreement amongst the future climate projections for the basin towards the end of the century.
Birt-hogg-Dubé Syndrome Incidentally Identified in a Potential Liver Donor
Authors: Gündoğdu Elif , Emekli Emre , Acer Ersoy , Özer İlter 
Published: December 2020

Background:
Birt-Hogg-Dubé Syndrome (BHDS), an autosomal dominant hereditary
condition, occurs due to mutations in the gene encoding folliculin (FLCN) in the short arm of the
17th chromosome characterized by lung cysts with specific skin findings and renal cell carcinoma.
Patients have usually complaints related to dyspnea and chest pain due to pneumothorax but they
may be asymptomatic due to wide phenotypic heterogeneity. Herein, we report the imaging findings
of a case 32-year-old male with BHDS without any symptom who was diagnosed incidentally
by computed tomography (CT) due to organ donation.

Case Report:
In a 32-year-old male patient evaluated as a potential liver donor, CT was performed
for preoperative preparation. The patient's medical history was unremarkable. In the CT examination,
multiple air cysts of different sizes in both lungs were observed and also, a 7-cm solid renal
mass of the right kidney was observed in the dynamic examination. Due to a large number of lung
cysts and the presence of solid renal tumors at a young age, BHDS was considered. The patient underwent
partial nephrectomy, and the pathology result was hybrid oncocytic-chromophobe renal
cell carcinoma. In the genetic examination, a heterozygous germline mutation was detected in the
11th exon of the FLCN gene.

Conclusion:
While potential organ donors are generally healthy and asymptomatic individuals, incidental
lesions can be detected in the donor organ or other organs in the examination area during
radiological imaging. Although most incidental lesions are benign, important clinical conditions
can rarely be observed, as in our case. Familial and syndromic conditions should also be considered
for the presence of solid renal masses incidentally detected at a young age. To the best of
our knowledge, this is the first reported case of BHDS in English literature who was diagnosed incidentally
on computed tomography for being a living liver donor.
Humor Training Program on Sense of Humor among Older Adults: A Randomized Controlled Trial
Authors: Momtaz Yadollah A. , Ansari Mobarake , Foroughan Mahshid 
Published: December 2020

Introduction:
Several studies have a well-documented positive association between sense of humor and physical and mental well-being in later life. However, there is evidence to indicate that sense of humor declines with age. This study was conducted to examine humor training program on the sense of humor among older adults.

Methods:
This community-based randomized controlled trial study was conducted in 2019. There were 18 older adults in the intervention group and 20 older adults in the control group. The intervention group received the 7 Humor Habits Program in eight training sessions, whereas the control group was waitlisted. The Multidimensional Sense of Humor Scale (MSHS) was used to measure the sense of humor before and after the intervention. A series of paired samples t-tests and difference-in-differences approach using IBM SPSS Version 23.0 were conducted to assess changes from pre-test to post-test. The significance level of p ≤ .05 was considered for the statistical analysis.

Results and Discussion:
The mean age of the participants in the intervention and control groups was 66.50, (SD=6.14) and 67.60, (SD= 5.64) years, respectively. The results of a series of paired samples t-tests revealed a statistically significant increase in the total score of sense of humor from pre-test (M =77.28, SD = 13.62) to post-test (M=101.11, SD=17.06), (t= -6.77, p<.001), in intervention group. Other paired samples t-tests showed statistically significant changes from pre-test to post-test for subscales of humor including enjoyment of humor (t= -4.59, p<.001), laughter (t= -7.83, p<.001), verbal humor (t= -4.73, p<.001), finding humor in everyday life (t= -4.19, p<.001), laughing at yourself (t= -6.36, p<.01), and humor under stress (t= -2.54, P≤.05) in intervention group. Moreover, the results of the difference-in-differences approach revealed a significant increase in the sense of humor for the intervention group compared to the control group (F=43.54, p<.001). No statistically significant changes were observed in any outcome variables in the control group.

Conclusion:
The results of this study provided support that the sense of humor can be improved using a training program in later life.
Frequency of reagent samples for syphilis in pregnant women treated at Distrital Leste Laboratory, Manaus, Amazonas, Brazil
Authors: Chagas Jessica da Cruz , Oliveira Aldiane Passos de , Andrade Cleudiane Pereira de , Silva Jander Torres da , Silva Edirany dos Santos , Cardoso de Sales Angela Cristina , Ferreira Rosilene Gomes da Silva 
Published: December 2020

Objective: Checking the frequency of reagent samples for syphilis in pregnant women treated at the Distrital Leste Laboratory in the city of Manaus from January 2016 to December 2018, comparing the positive VDRL (Venereal Disease Research Laboratory) cases with the confirmatory FTA-Abs (Fluorescent Treponemal Antibody Absorption Test). Methods: The study was cross-sectional and assessed the results of two syphilis diagnostic tests, VDRL and FTA-Abs. Data were obtained using the database of the electronic program SoftLab® and the Laboratory Environment Manager (GAL) and were quantitatively analyzed. Results: In 2016, 9,028 VDRL tests were performed on pregnant women, 8,562 tests in 2017 and 5,064 in 2018. From this total, 1020 tested positive for syphilis; 392 in 2016, 320 in 2017 and 308 in 2018, increasing from 4 to 6%. Comparison with the FTA-Abs was only made possible in 2016 when the diagnosis was confirmed in 82% of the tests. Conclusion: It was possible to check the frequency of pregnant women with positive and confirmatory results from 2016 to 2018, showing that although this pathology is easily preventable, it remains a challenging public health issue in pregnant women.
Occurrence of oral mucositis in women during oncological treatment of breast cancer in the Brazilian Northeast
Authors: Barbosa-Lima Ricardo , Kameo Simone Yuriko , Amorim Bruno Ferreira , Ramos Maria Júlia Oliveira , Costa Jéssica dos Santos , Marinho Pabliane Matias Lordelo , Sawada Namie Okino , Moura Silva Glebson 
Published: December 2020

Objective: To describe the occurrence of oral mucositis (OM) in women undergoing cancer treatment for breast cancer (BC). Methods: This is a retrospective, quantitative, and analytical study in medical records of women diagnosed with BC in an oncology service. Clinical data were collected regarding the occurrence and severity of OM according to the World Health Organization criteria and the cancer treatment experienced by the participants. Results: 196 women were included. Of these, 97 (49.5%) developed OM, 43.4% of which were grade 1 or 2 (low or moderate). The occurrence was higher in white women (OR 1.93; 95% CI 1.04 - 3.57; p = 0.035), with metastatic breast cancer (OR 5.46; 95% CI 1.79 - 16.64; p = 0.002) and who experienced taxane agents at some point during chemotherapy (OR 2.26; 95% CI 1.12 - 4.56; p = 0.02). The mean severity of OM in the entire sample was 0.8 ± 1.0, and in the affected women was 1.7 ± 0.7. The difference in the severity of OM by the variables was observed only among women with grade 2 and grade 3 fatigue (p = 0.03). Conclusions: OM is a common mucocutaneous toxicity in women with BC. Despite the low severity observed, care for women with BC undergoing cancer treatment must consider the possible risks and complications associated with OM, adopting strategies to prevent, monitor, and treat them.
Efeitos da prática de caminhada em hipertensos: revisão integrativa
Authors: Teixeira Letícia Queiroz , Aguiar Oliveira Diogo Muryel , Silva Kácio dos Santos 
Published: December 2020

Objetivo: analisar os efeitos da prática da caminhada em hipertensos. Métodos: revisão integrativa da literatura em língua portuguesa, efetuada nas bases de dados SciELO e Lilacs – BVS. Realizou-se uma revisão em sete artigos publicados entre os anos de 2010 e 2020. Resultados: o estudo revelou que a prática da caminhada é efetiva para redução dos níveis pressóricos em diferentes contextos e intensidades no controle da hipertensão, seja com efeitos agudos e/ou crônicos, sendo assim considerada uma ferramenta não medicamentosa, pois condiciona o sistema cardiovascular a responder fisiologicamente com diminuição da reatividade vascular, mesmo em situações de estresse, induzindo a diminuição na pressão arterial. Conclusão: a pesquisa apontou que a caminhada tem efeitos positivos sobre os níveis pressóricos, uma vez que a mesma contribuiu significativamente para o controle da hipertensão arterial sistêmica, especialmente naquelas que tiveram acompanhamento de um profissional de educação física. Sugere-se a proposição de novas pesquisas que levem em consideração não só a atuação desse profissional, mas também utilize outras variáveis, como diferentes modalidades de atividade física, dieta alimentar, níveis de estresse e qualidade do sono.
Using a Tablet-Based App to Deliver Evidence-Based Practices for Suicidal Patients in the Emergency Department: Pilot Randomized Controlled Trial
Authors: Dimeff Linda A , Jobes David A , Koerner Kelly , Kako Nadia , Jerome Topher , Kelley-Brimer Angela , Boudreaux Edwin D , Beadnell Blair , Goering Paul , Witterholt Suzanne , Melin Gabrielle , Samike Vicki , Schak Kathryn M 
Published: December 2020

Background
Emergency departments (EDs) have the potential to provide evidence-based practices for suicide prevention to patients who are acutely suicidal. However, few EDs have adequate time and personnel resources to deliver recommended evidence-based assessment and interventions. To raise the clinical standard of care for patients who are suicidal and seeking psychiatric crisis services in the ED, we developed Jaspr Health, a tablet-based app for direct use by such patients, which enables the delivery of 4 evidence-based practices.

Objective
This study aims to evaluate the feasibility, acceptability, and effectiveness of Jaspr Health among suicidal adults in EDs.

Methods
Patients who were acutely suicidal and seeking psychiatric crisis services participated in an unblinded pilot randomized controlled trial while in the ED. Participants were randomly assigned to Jaspr Health (n=14) or care as usual (control; n=17) groups. Participants were assessed at baseline, and a 2-hour posttest using self-report measures and a semistructured interview were conducted.

Results
Conditions differed significantly at baseline with regard to age but not other demographic variables or baseline measures. On average, participants had been in the ED for 17 hours before enrolling in the study. Over their lifetime, 84% (26/31) of the sample had made a suicide attempt (mean 3.4, SD 6.4) and 61% (19/31) had engaged in nonsuicidal self-injurious behaviors, with an average rate of 8.8 times in the past 3 months. All established feasibility and acceptability criteria were met: no adverse events occurred, participants’ app use was high, Jaspr Health app user satisfaction ratings were high, and all participants using Jaspr Health recommended its use for other suicidal ED patients. Comparisons between study conditions provide preliminary support for the effectiveness of the app: participants using Jaspr Health reported a statistically significant increase in receiving 4 evidence-based suicide prevention interventions and overall satisfaction ratings with their ED experience. In addition, significant decreases in distress and agitation, along with significant increases in learning to cope more effectively with current and future suicidal thoughts, were observed among participants using Jaspr Health compared with those receiving care as usual.

Conclusions
Even with limited statistical power, the results showed that Jaspr Health is feasible, acceptable, and clinically effective for use by ED patients who are acutely suicidal and seeking ED-based psychiatric crisis services.

Trial Registration
ClinicalTrials.gov NCT03584386; https://clinicaltrials.gov/ct2/show/NCT03584386
A Semiautomated Classification System for Producing Service Directories in Social and Health Care (DESDE-AND): Maturity Assessment Study
Authors: Romero-Lopez-Alberca Cristina , Alonso-Trujillo Federico , Almenara-Abellan Jose Luis , Salinas-Perez Jose A , Gutierrez-Colosia Mencia R , Gonzalez-Caballero Juan-Luis , Pinzon Pulido Sandra , Salvador-Carulla Luis 
Published: December 2020

Background
DESDE-LTC (Description and Evaluation of Services and DirectoriEs for Long-Term Care) is an international classification system that allows standardized coding and comparisons between different territories and care sectors, such as health and social care, in defined geographic areas. We adapted DESDE-LTC into a computer tool (DESDE-AND) for compiling a directory of care services in Andalucia, Spain.

Objective
The aim of this study was to evaluate the maturity of DESDE-AND. A secondary objective of this study is to show the practicality of a new combined set of standard evaluation tools for measuring the maturity of health technology products.

Methods
A system for semiautomated coding of service provision has been co-designed. A panel of 23 domain experts and a group of 68 end users participated in its maturity assessment that included its technology readiness level (TRL), usability, validity, adoption (Adoption Impact Ladder [AIL]), and overall degree of maturity [implementation maturity model [IMM]). We piloted the prototype in an urban environment (Seville, Spain).

Results
The prototype was demonstrated in an operational environment (TRL 7). Sixty-eight different care services were coded, generating fact sheets for each service and its geolocation map. The observed agreement was 90%, with moderate reliability. The tool was partially adopted by the regional government of Andalucia (Spain), reaching a level 5 in adoption (AIL) and a level 4 in maturity (IMM) and is ready for full implementation.

Conclusions
DESDE-AND is a usable and manageable system for coding and compiling service directories and it can be used as a core module of decision support systems to guide planning in complex cross-sectoral areas such as combined social and health care.
Pengembangan dan Perkembangan Pemikiran Hukum Pertanian di Indonesia
Authors: Moeliono Tristam Pascal , Soetoprawiro Koerniatmanto 
Published: December 2020

Agricultural law is an emerging specialized field in Indonesian legal discourse and the public as well as academics has yet to get acquainted with this new perspective. The importance of agricultural law should be placed in relation with the fact that the Indonesian government, even after more then 75 year of independence, did not seriously address the plight of peasants and fishermen, living in rural areas throughout Indonesia. Agricultural law as a perspective is developed to bring back the focus of national development to agriculture in its widest sense. The focus of analysis of this approach will be on policies and rules regarding agriculture and how it affects peasants-farmers working in informal-semi formal setting as well those working in the agroindustry and business. Other basic concepts employed relates to ecological-social justice, food sovereignty, security, and safety. Hopefully, this new approach to human development and agriculture may contribute to a better analysis of the plight of agriculture in Indonesia and how to improve this particular sector.
Abstrak
Hukum pertanian sebagai suatu kajian belum begitu dikenal dalam khasanah ilmu hukum Indonesia. Masyarakat umum, akademisi dan praktisi hukum masih harus berkenalan dengan pendekatan ini. Pentignya hukum pertanian harus ditempatkan dalam konteks kurangnya kepedulian rezim pemerintah Indonesia, bahkan dalam kurun waktu 75 tahun sejak merdeka, pada pembangunan sektor pertanian rakyat. Hukum pertanian merupakan satu upaya untuk mendorong kehidupan pertanian di Indonesia dan mayoritas masyarakat petani (bukan semata-mata perkebunan besar) menjadi bagian dari kehidupan modern yang manusiawi. Fokus kajian hukum pertanian adalah kebijakan dan aturan hukum yang diterbitkan pemerintah dari waktu ke waktu di bidang pertanian dalam arti luas yang bersentuhan dengan kehidupan petani-nelayan, mereka yang bekerja di sektor-sektor informal maupun semi formal sampai dengan agrobisnis dan industri. Sebagai titik tolak, digunakan konsep-konsep dasar keadilan sosial-ekologi, ketahanan-kedaulatan dan keamanan pangan. Salah satu sumbangan yang diharapkan adalah munculnya perspektif pemikiran dan solusi konstruksif yang baru untuk membangun manusia serta agrikultur dalam rangka menangani persoalan kemiskinan dan ketidakadilan sosial di Indonesia.