bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cancer cells invasion to the gastric bare area adipose tissue: a poor prognostic predictor for gastric cancer
Authors: Chen Yongming , Xu Shuhang , Huang Chunyu , Ling Yihong , Liang Chengcai , Miao Yuhua , Sun Xiaowei , Li Yuanfang , Zhou Zhiwei 
Published: October 2020

Abstract
Background: The relationship between gastric bare area adipose tissues invasion (GBAI) confirmed pathologically and the prognosis of gastric cancer (GC) patients is undefined. Till present, there hasn’t been literature investigating this phenomenon. Here, we aimed at analyzing the implication of GBAI in GC.

Methods: The data of 1822 patients who underwent radical surgery between January 2000 and December 2013 at the Sun Yat-sen University Cancer Center were retrieved. Pathologically, tumor deposits (TDs) located >5mm from the leading edge of the primary tumor and the lymph nodes (LNs) station number 1, 2, 7, and 9 were considered as GBAI. Kaplan-Meier method, log-rank test and Cox’s proportional hazards model were employed to analyze.

Results: 205 (11.3%) patients were pathologically diagnosed with GBAI, which was more commonly found in proximal or linitis lastica than distal GC (P<0.001). There was significant difference in 5-year survival between patients with and without GBAI for stages IIB, IIIA, IIIB, IIIC, respectively (P<0.009 for IIB, IIIA and IIIB, P=0.021 for IIIC). Among the 205 GBAI patients, 61 had detailed radiological follow-up data in which 26 (34.7%) were found to have retroperitoneal infiltration, 27 (36.0%) had peritoneal metastasis, 10 (13.3%) had hematogenous metastasis, 16 (21.3%) had lymphatic metastasis, and 16 (21.3%) had others.

Conclusions: GBAI was identified as a predictor of unfavourable prognosis for GC and was more commonly found in the proximal or linitis plastica of the stomach than in distal stomach. Retroperitoneal infiltration was one of the most commonly identified metastatic route for GC associated with GBAI after radical surgery.
Leukemia mortality in children from Latin America: Trends and predictions to 2030
Authors: Torres-Roman J. Smith , Valcarcel Bryan , Guerra-Canchari Pedro , Santos Camila Alves Dos , Barbosa Isabelle Ribeiro , Vecchia Carlo La , McGlynn Katherine A. , de Souza Dyego Leandro Bezerra 
Published: October 2020

Abstract
Background: Reports suggest that Latin American and Caribbean (LAC) countries have not reduced in leukemia mortality compared to high-income countries. However, updated trends remain largely unknown in the region. Given that leukemia is the leading cause of cancer-related death in LAC children, we evaluated mortality trends in children (0-14y) from 15 LAC countries for the period 2000-2017 and predicted mortality to 2030.Methods: We retrieved cancer mortality data using the World Health Organization Mortality Database. Mortality rates (standardized to the world standard SEGI population) were analyzed for 15 LAC countries. We evaluated the average mortality rates for the last 5 years (2013-2017). Joinpoint regression analysis was used to evaluate leukemia mortality trends and provide an estimated annual percent change (EAPC). Nordpred was utilized for the calculation of predictions until 2030.Results: Between 2013 and 2017, the highest mortality rates were reported in Venezuela, Ecuador, Nicaragua, Mexico, and Peru. Upward mortality trends were reported in Nicaragua (EAPC by 2.9% in boys, and EAPC by 2.0% in girls), and Peru (EAPC by 1.4% in both sexes). Puerto Rico experienced large declines in mortality among both boys (EAPC by −9.7%), and girls (EAPC by −6.0%). Leukemia mortality will increase in Argentina, Ecuador, Guatemala, Panama, Peru, and Uruguay by 2030.Conclusion: Leukemia mortality is predicted to increase in some LAC countries by 2030. Interventions to prevent this outcome should be tailor to reduce the socioeconomic inequalities and ensure universal healthcare coverage.
Early change in prostate-specific antigen levels as a predictive marker for overall survival during vintage hormonal therapy for metastatic hormone-sensitive prostate cancer
Authors: Nakanishi Shotaro , Goya Masato , Tamaki Mitsuyoshi , Oshiro Takuma , Saito Seiichi 
Published: October 2020

Abstract
Background

The effect of early changes in prostate-specific antigen (PSA) levels after androgen deprivation therapy (ADT) in metastatic hormone-sensitive prostate cancer (mHSPC) patients has not been well investigated. Here, we evaluated the effect of factors that lead to castration-resistant prostate cancer (CRPC) progression and overall survival (OS) in mHSPC.
Methods

Medical records of 71 consecutive primary mHSPC patients treated with ADT were analyzed. Factors predicting the time to CRPC and OS in these patients were evaluated at 3 months after ADT induction.
Results

The median times to CRPC and OS were 15 months and 92 months, respectively. In multivariate analysis using Cox proportional hazards regression, a Gleason score of 8 or more (p = 0.004), extent of disease value (EOD) of 2 or more (p = 0.004), and a PSA level of 1% or more of the pretreatment levels after 3 months of ADT (p = 0.017) were independent predictors of shorter time to CRPC. For OS, a PSA level of 1% or more after 3 months of ADT was the independent predictor (p = 0.004).
Conclusion

% PSA was an important factor that correlated with poor prognosis at 3 months after ADT induction.
Safety and Efficacy of Hemispherical with Flattened Pole Dual-Mobility Acetabular Cup in Revision or Complex Hip Arthroplasty: The SYMCOR-2 study
Authors: Estour Gilles , Bonin Nicolas , Loïc Schneider , Guyen Olivier , Daoud Frederic Christopher 
Published: October 2020

Abstract
Background: This study estimated the short-term clinical safety and efficacy of hemispherical with flattened pole cobalt-chromium metal-back dual-mobility acetabular cup with porous outer hydroxyapatite coating and anchoring screw and pods (HFPC-DMR-HA) or cement fixation (HFPC-DM-CEM), in revision or complex total hip arthroplasty.Methods: Single-center retrospective observational cohort study of consecutively operated patients who underwent THA with an HFPC-DMR-HA or HFPC-DM-CEM cup 2 years prior to study start. Prospective 2-year follow-up with letter and phone questionnaires.Results: Sampling frame: 203 patients including 9.85% in the two cohorts with 15 HFPC-DMR-HA and 5 HFPC-DM-CEM. 3 (20%) and 2 (40%) patients were lost to follow-up, respectively. Median age was 85.6 years (range: 45.1 ; 93.3) and 78.8 (range: 68.8 ; 86.9). Median follow-up (years): HFPC-DMR-HA: 2.3, HFPC-DM-CEM: 3.3. Clinical indications: HFPC-DMR-HA 67% revision & 33% primary THAs, HFPC-DM-CEM 100% revision. Primary endpoint: 2-year implant survival rate: HFPC-DMR-HA 93% [59, 99], HFPC-DM-CEM 100%. Prosthetic dislocation: HFPC-DMR-HA 1 (6.7%), HFPC-DM-CEM 0%. Secondary endpoint: Modified HHS (pain & functional subscore) improved with HFPC-DMR-HA from baseline 26.8 [14.9, 38.7] to 82.2 [73.5, 90.9] at 2-year follow-up (p<0.0001); HFPC-DM-CEM from 41.6 [24.9, 58.3] to 80.7 [55.8, 100].Conclusions: The authors deemed the short-term outcomes with these acetabular cups in revision or complex total hip arthroplasty to be satisfactory.Study registration: clinicaltrials.gov NCT04209426.
Does Organizational Vision Really Matter? An Empirical Examination of Factors Related to Organizational Vision Integration Among Hospital Employees
Authors: Slåtten Terje , Mutonyi Barbara Rebecca , Lien Gudbrand 
Published: October 2020

Abstract
Background: There seems to be a consensus that a vision for an organization is a valuable thing for organizations to have. However, research on organizational vision has predominantly been studied from a leadership perspective. In contrast to previous research, organizational vision in this paper takes an employee perspective. Specifically, the purpose is to examine factors associated with the integration of organizational vision among employees in hospital organizations. Consequently, it focuses on a relatively neglected domain within health services research.Methods: A conceptual model, centred on the concept of organizational vision integration, was developed and tested on a sample (N = 1008) consisting of hospital employees. Partial least-squares structural equation modelling (PLS-SEM) was used to test the hypotheses, using SmartPLS 3 software. Furthermore, a bootstrapping test was used to inspect potential mediating effects. Specifically, the test assessed whether the proposed direct and indirect effects were statistically significant, and at the same time revealed the nature of the mediation effect.Results: The results from the empirical study reveal three key findings: i) organizational vision integration among employees is directly and positively related to creative performance in their respective work role (β = 0.16). Organizational vision integration and employees’ psychological capital explains almost 40% (R2 = 0.37) in employees’ creative performance, ii) psychological capital and employees’ perception of organizational attractiveness are directly and positively related to employees’ organizational vision integration (β = 0.19 and β = 0.41, respectively) and explains about 30% (R2 = 0.29) of employees’ organizational vision integration, iii) employees’ organizational vision integration mediates the relationship between employees’ psychological capital, perception of organizational attractiveness and employees’ creative performance.Conclusions: Taking an employee perspective, this study contributes to revealing whether and how organizational vision matters and its impact on hospital employees’ work performance. To achieve organizational vision integration among hospital employees successfully, this study shows that it is important for hospital leaders to be aware of the pattern of impact of both personal as well as environmental-related factors.
Ruanjian Sanjie Decoction Induces Apoptosis in Triple-negative Breast Cancer Cells by Downregulating cIAP1/2 and XIAP
Authors: Zhao Xiumei , Wang Tongxing , Jia Qiang , Wang Luyao , Tan Cheng , Shen Hongsheng , Shi Pengcheng , Liu Lei , Li Wenchang , Hu Yunhui , Liu Hongbin 
Published: October 2020

Abstract
Background: Traditional Chinese medicine (TCM) comprises a unique theoretical system developed over thousands of years. The previous study reported that Ruanjian Sanjie (RJSJ) exerts anti-tumor effects by inducing cell apoptosis. However, the mechanism is not clear. Methods: In this study, we investigated the possible mechanism by the strategy of combining network pharmacology analysis with experiment (in vitro and in vivo). First, four kinds of breast cancer cell lines were used to conduct proliferation, apoptosis and cell cycle analysis. Secondly, to study pathophysiological processes of breast cancer at the molecular network level, we for the first time constructed an “integrated apoptosis module network of breast cancer” by assembling the regulatory relationships of canonical apoptosis signaling pathways. Through the strategy of combining network analysis and experiments, we analyzed the main mechanism of RJSJ in breast cancer and screened out the core genes. We further studied the inhibitory effect of RJSJ combined with carboplatin (CBP) in vivo. Finally, the synergistic effect of RJSJ and CBP were analyzed and the potential active components in RJSJ were predicted.Results: This study demonstrated that RJSJ could significantly inhibit breast cancer cell proliferation and induce apoptosis in a concentration-dependent manner. The primary mechanism of RJSJ in the treatment of breast cancer was pro-apoptotic. The core apoptosis genes regulated by RJSJ were cIAP1/2 and XIAP. We also found that RJSJ in combination with CBP tended to synergistically induce apoptosis, which might mainly be achieved through the regulation of multiple targets and pathways. Alexandrin (BX05, XKC02, SCG01), baicalin (BX22), guanosine (BX32), arjunglucoside I (XKC10) etc. were predicted as potential active components.Conclusions: These findings provide the rationale for exploring the therapeutic effects of RJSJ against breast cancer and providing a bridge for the combined use of Chinese and Western medicine.
Dissect the Heterogeneity of Longitudinal Cognitive Declines with Risk Factors and Functional Outcomes in Bipolar Disorder
Authors: Chen Wen Yin , Huang Ming-Chyi , Lee Ya-Chin , Chang Chiao-Erh , Lin Shih-Ku , Chiu Chih-Chiang , Liu Hsing-Cheng , Kuo Chian-Jue , Chen Po-Yu , Kuo Po-Hsiu 
Published: October 2020

Abstract
Background: We characterized the heterogeneity and risk factors of cognitive decline in bipolar disorder (BD), and their magnitude of associations with subjective daily functions. Methods: Recruited BD patients (N=127) were followed for an average of 6.5 years. Baseline intelligence quotient was measured, and Brief Assessment of Cognition in Affective Disorders (BAC-A) was used to assess cognition at follow-up. We evaluated current functions with World Health Organization Disability Assessment Schedule 2.0. Clinical and sociodemographic factors were compared between BD patients with and without longitudinal cognitive decline and revealed the independent risk factors through multiple regression analysis. Correlations between specific cognitive domains and functional outcomes were examined in subgroups of BD.Results: Overall, 32.3% of BD patients showed longitudinal cognitive decline. In cognitive decline group, all cognitive domains of BAC-A were significantly worsened. Baseline independent risk factors for cognitive decline were older age, body mass index >25, and manic episode density during the disease course. After the age of 40 years, a steeper cognitive change was observed in the cognitive decline group. Housekeeping dysfunction was the most sensitive function related to diverse cognitive impairment in cognitive decline group. The correlations between cognitive domains and functional outcomes differed between patients with and without cognitive decline.Conclusions: This study characterized cognitive heterogeneity longitudinally in BD, as the foundation to facilitate future genetic and biological studies for BD subtypes. Manic episode density and overweight are important risk factors for cognitive decline; therefore, we suggested further interventions should target on relapse prevention and controlling body weight.
Awareness of Sudanese Citizens about the Benefits of Breastfeeding compared to Formula in Khartoum State, Sudan
Authors: Aljafari Abdulmalek , Aziz Ibrahim Adel , Hezima Ahmed Gamal , Awad Mustafa Sabir , AlMustafa Ahmed , Sammarraie Ahmad Khairi Al , Alhaboob Ahmed Mohammedahmed , Hamadnalla Mohanad Altigani 
Published: October 2020

Abstract
Background: There are a major differences between breastfeeding and formula feeding. Therefore, it’s essential for expectants and new parents to be aware of such variations, as its important determinants of infant feeding behavior. This study aimed to construct a solid foundation on the awareness and perception regarding breastfeeding verses packed formulas among the locals. Additionally, we wished to identify any possible misconceptions about their benefits.Methods: This is a cross-sectional descriptive study, carried out during the months of July and August 2020 at Khartoum state, Sudan. The target population was all Sudanese citizens of both genders, aged 18 and above, who’ve agreed to participate in this study, respondents who’ve missed any of the aforesaid criteria were excluded. Results: Among our study respondents (n=905), 464 (51.27%) were female and 409 (45.19%) were married. Two hundred and eleven (23.31%) had 1-2 children and 422 (46.63%) held a bachelor’s degree. The mean awareness score was 10.88 (SD: 2.25, range: 0-15), suggesting an overall 72.53% (10.88/15*100) correct rate on this awareness test. Awareness scores significantly differed across genders, age-groups, marital status, education levels, and places of residence (P<0.05). Most of our sample (97.35%) agreed that breastfeeding is the ideal nutrition for infants <6 months old. Five hundred and ninety (65.19%) were aware that breastfeeding reduces the mortality rate of infants, and 712 (78.67%) of the participants answered correctly that breastfeeding decreases the maternal risk of breast and ovarian cancer.Conclusion: Our findings suggest that the general awareness on this topic needs further improvement and more attention.
Circulating Tumor Cell and Metabolites as Novel Biomarkers for Early-stage Lung Cancer Diagnosis
Authors: Wan Lingling , He Yutong , Liu Qingyi , Liang Di , Guo Yongdong , Liu Guangjie , Ren Jinxia , Shan Baoen 
Published: October 2020

Abstract
Background: Lung cancer is a malignant tumor that has the highest morbidity and mortality rate among all cancers. Early diagnosis of lung cancer is a key factor in reducing mortality and improving prognosis. Methods: In this study, we performed CTC next-generation sequencing (NGS) in early-stage lung cancer patients to identify lung cancer-related gene mutations. Meanwhile, a serum liquid chromatography-tandem mass spectrometry (LC-MS) untargeted metabolomics analysis was performed in the CTC-positive patients, and the early diagnostic value of these assays in lung cancer was analyzed. Results: 62.5% (30/48) of lung cancer patients had ≥ 1 CTC. By CTC NGS, we found that > 50% of patients had 4 commonly mutated genes, namely, NOTCH1, IGF2, EGFR, and PTCH1. 47.37% (9/19) patients had ARIDH1 mutations. Additionally, 30 CTC-positive patients and 30 healthy volunteers were subjected to LC-MS untargeted metabolomics analysis. We found 100 different metabolites, and 10 different metabolites were identified through analysis, which may have potential clinical application value in the diagnosis of CTC-positive early-stage lung cancer (AUC > 0.9). Conclusions: Our results indicate that NGS of CTC and metabolomics may provide new tumor markers for the early diagnosis of lung cancer. This possibility requires more in-depth large-sample research for verification.
Cross sign T2 hyperintensities in atrophic spinal cord of hereditary spastic paraplegia type 5
Authors: Wang Ning , Liu Ying , Ye Zhixian , Lin Yi , Lin Xiang , Chen Yi-Jun , Wang Meng-Wen , Hu Jianping , Cai Wenjie , Weng Qiang , Zeng Yi-Heng , Li Gui-He , Huang Xue-Jing , Liu Yaou , Chen Wan-Jin , Cao Dairong , Fu Ying 
Published: October 2020

Abstract
Background: Hereditary spastic paraplegias type 5 (SPG5) is an inherited neurodegenerative disease with 27-hydroxycholesterol abnormal accumulation. Imaging and pathologic manifestations remain poorly understood due to the rare incidence. This study reveals the MRI features of SPG5, and aims to investigate a promising imaging diagnostic biomarker for SPG5.Methods: We prospectively recruited SPG5 patients and matched healthy controls from Neurogenetic Diseases Centers of Fujian province in China, clinical and MRI data of whom were collected. Abnormalities of spinal cord and brain were characterized and quantified by conventional and quantitative MRI. Comparisons were conducted between MRI and cerebrospinal fluid (CSF) bioindicators.Results: Seventeen SPG5 patients were enrolled (11 men, 6 women; age range, 13–49 years; median disease duration, 14 years). For the first time, T2 hyperintensities with “+” form (cross sign) in atrophic spinal cord was found among all SPG5 patients. To grade severity of this sign, we set up a scoring scale (cross-sign scores) in cervical spinal cords. Unexpectedly, total cross-sign scores showed a strong positive correlation with disability scale scores (r = 0.687, P = 0.002) and disease duration (r = 0.520, P = 0.032). Although total spinal cord area was reduced (cervical levels: 12-27%; thoracic levels 41-60%), no correlation was found between spinal cord atrophy and disease severity. In CSF, a positive correlation was identified between 27-hydroxycholesterol and neurofilament light (r = 0.468, P = 0.049), although 27-hydroxycholesterol and neurofilament light were unrelated to disease severity.Conclusion: Cross sign of spinal cord was established as a potentially diagnostic biomarker linked to SPG5 that can guide genetic testing and interpret genetic results. Moreover, cross-sign scoring scale is more sensitive than spinal cord area and CSF markers for monitoring SPG5 progress in our research.
Shockwave-induced DNA-free genome editing in tobacco: targeting the actin depolymerizing factor gene increases drought and salinity tolerance
Authors: Augustine Sruthy Maria , Cherian Anoop Vadakan , Seiling Kerstin , Fiore Stefano Di , Raven Nicole , Commandeur Ulrich , Schillberg Stefan 
Published: October 2020

Abstract
DNA-free genome editing involves the direct introduction of ribonucleoprotein (RNP) complexes into cells, but this strategy has rarely been successful in plants. Here we describe a new technique for the introduction of RNPs into plant cells involving the generation of cavitation bubbles using a pulsed laser. The resulting shockwave achieves the efficient transfection of walled cells in tissue explants by the creation of transient membrane pores. RNP-containing cells were rapidly identified by fluorescence microscopy, followed by regeneration and the screening of mutant plants by high-resolution melt analysis. We used this technique in tobacco to target the endogenous phytoene desaturase (pds) and actin depolymerizing factor (adf) genes. Genome-edited plants were produced with an efficiency of 5.6–8.7%. We also evaluated the effects of adf mutations in T2 mutant plants under drought and salinity stress, showing that adf acts as a key regulator of osmotic stress tolerance in plants.
Computational analyses of the G476S variant of SARS-CoV-2: A focus on the interaction with human ACE-2 and neutralizing antibodies
Authors: Kwarteng Alexander , Asiedu Ebenezer , Sylverken Augustina Angelina 
Published: October 2020

Abstract
Recently, several mutations in the SARS-CoV-2 genome have been identified and reported. However, little is currently known about the influence of these mutations on the infectivity, transmissibility and antigenicity of the virus. Here, using an integrative computational approach, we characterized the G476S variant of SARS-CoV-2 focusing on interactions with ACE-2 and neutralizing antibodies. The substitution of Gly-476 to Ser-476 in the SARS-CoV-2 Receptor-binding domain (RBD) largely affected the structural dynamics of the S-protein leading to significant influence on the interactions with ACE-2 and neutralizing antibodies. Structural properties of the S-protein such as conformation changes, residual fluctuations and residue surface area largely varied between the wild-type and G476S variant, especially in the RBD. Analyses of the interaction energies between S-protein and ACE-2 suggest that the G476S variant may have enhanced interactions with ACE-2 compared to the wild-type. The G476S variant was found to have weaker interactions with the neutralizing antibody H014 compared to the wild-type. Collectively, our findings have implications for the infectivity and antigenicity of the G476S variant of SARS-CoV-2.