bluuebunny/crossref_metadata_2025_split huggingface dataset:
‘Hand on hip’ sign: A novel screening test and diagnostic tool in piriformis syndrome
Authors: Sadiq Mohammed , Hussain Syed Azher , Meganath P 
Published: October 2020

Introduction: Currently there is no gold standard clinical test or investigation available to diagnose piriformis syndrome. We have found a pose used by the patients to describe the pain in the gluteal region in cases of suspected piriformis syndrome. This pose in our study has been described as ‘hand on hip sign’ (HHS). In this study, we aim to assess the significance of this sign in screening of patients with piriformis syndrome. Materials and methods: Seventy patients presenting with pain in the gluteal region with radiculopathy between the age group of 18 years and 60 years were included. All patients were asked to locate the site of the pain in the gluteal region. The demonstration of pain by the patients was noted. If the description matched the description of HHS, it was considered to be positive, else it was considered negative. All patients were subjected to four piriformis-specific confirmatory clinical tests. Considering that there is no single confirmatory test, any patient in whom any two piriformis-specific signs were elicited was considered as a case of piriformis syndrome. Using the data, sensitivity, specificity and positive and negative likelihood ratio of the HHS in the diagnosis of piriformis syndrome were calculated. Inter-test comparison was also made using Cohen’s kappa statistic. Results: HHS was found to have a sensitivity of 86% and a specificity of 75%. It also has a high positive predictive value of 94% and a positive likelihood ratio of 3.44. Inter-test reliability comparison showed that there was a fair to moderate agreement between HHS and other confirmatory piriformis-specific tests. Conclusion: HHS serves as an effective screening test in the identification of piriformis syndrome.
Perception of Protective Stabilization by Pediatric Dentists: A Qualitative Study
Authors: Marty M. , Marquet A. , Valéra M.C. 
Published: October 2020

Introduction: Pediatric dentists sometimes have to care for children who refuse to cooperate with the oral examination or dental treatment. Behavior management strategies are used, such as “tell-show-do,” distraction, and positive reinforcement. Anxiety management can also be performed by the use of conscious sedation (oral premedication, nitrous oxide/oxygen inhalation). Unfortunately, these techniques are sometimes insufficient for providing oral care, and protective stabilization may be an option in some situations. Little is known on the impact of physical restraint and how practitioners feel about it. The objective of this study was to evaluate the perception of dentists using protective stabilization for dental care in children. Methods: Semistructured qualitative interviews on the perception of pediatric dentists concerning protective stabilization were conducted in the pediatric dentistry department of the University Hospital of Toulouse, France. A thematic analysis of interview transcripts was provided via NVivo software. Results: This analysis highlighted 3 main themes. First, the perceptions of dentists concerning protective stabilization showed that this procedure has a major psychological impact and led to a feeling of professional failure. Second, the reasons for which the child was stabilized were described; these concerned the child (behavior, age, number of treatments) and the environment (the parents and the medical team). Finally, we detailed how dentists manage the effects of using of protective stabilization. Conclusion: Dental surgeons must balance their requirement to make concrete decisions regarding the provision of care with their personal convictions about protective stabilization. This study also shows the need for specific training on this subject, as well as the desire of certain dentists that public authorities implement legislation on this matter. Knowledge Transfer Statement: The findings of this study will improve the management of young patients by identifying situations where protective stabilization may be useful (age of the child, diagnosis, protection of the child or the medical team), while showing its psychological impact on practitioners. Finally, this work provides a basis for decision makers to propose a framework for the use of physical restraint.
Clinical and vascular responses to propranolol and candesartan in migraine patients: A randomized controlled clinical trial
Authors: Barzenje Aros Dlawer , Gjesdal Knut , Winsvold Bendik Slagsvold , Småstuen Milada Cvancarova , Stovner Lars Jacob , Gravdahl Gøril Bruvik , Nilsen Kristian Bernhard 
Published: October 2020

Background: Both propranolol and candesartan are prophylactic drugs for migraine, but with unknown mechanisms of action. The objectives of the present study were to investigate these drugs’ effects on arterial wall dynamics and the potential relation between their vascular and clinical effect. Methods: The study was based on data from a previously published randomized, placebo-controlled, triple-blinded, double crossover clinical trial comparing the prophylactic effects of candesartan and propranolol in 72 patients. Finapres noninvasive blood pressure curves were analyzed. On the descending limb of the pulse curve, a notch is produced by pulse wave reflection, and its relative height compared to the top of the curve (the notch ratio) was used as a marker of arterial wall stiffness. Results: Candesartan decreased the notch ratio from baseline ( p = 0.005), reflecting more compliant arteries and vasodilation, whereas propranolol increased the notch ratio ( p = 0.005), reflecting less compliant arteries and vasoconstriction. There was no difference in baseline notch ratio between clinical responders and nonresponders. Conclusion: The drugs are both efficient prophylactic medications, yet they have opposite effects on arterial wall dynamics. This suggests that drug effects other than those on arterial compliance must be responsible for their prophylactic effect in migraine.
MYD88 L265P elicits mutation-specific ubiquitination to drive NF-κB activation and lymphomagenesis
Authors: Yu Xinfang , Li Wei , Deng Qipan , Liu Haidan , Wang Xu , Hu Hui , Cao Ya , Xu-Monette Zijun Y. , Li Ling , Zhang Mingzhi , Lu Zhongxin , Young Ken H. , Li Yong 
Published: October 2020

Abstract
Myeloid differentiation primary response protein 88 (MYD88) is a critical universal adapter that transduces signaling from Toll-like and interleukin receptors to downstream nuclear factor-κB (NF-κB). MYD88L265P (leucine changed to proline at position 265) is a gain-of-function mutation that occurs frequently in B-cell malignancies such as Waldenstrom macroglobulinemia. In this study, E3 ligase RING finger protein family 138 (RNF138) catalyzed K63-linked nonproteolytic polyubiquitination of MYD88L265P, resulting in enhanced recruitment of interleukin-1 receptor–associated kinases and elevated NF-κB activation. However, RNF138 had little effect on wild-type MYD88 (MYD88WT). With either RNF138 knockdown or mutation on MYD88 ubiquitination sites, MYD88L265P did not constitutively activate NF-κB. A20, a negative regulator of NF-κB signaling, mediated K48-linked polyubiquitination of RNF138 for proteasomal degradation. Depletion of A20 further augmented MYD88L265P-mediated NF-κB activation and lymphoma growth. Furthermore, A20 expression correlated negatively with RNF138 expression and NF-κB activation in lymphomas with MYD88L265P and in those without. Strikingly, RNF138 expression correlated positively with NF-κB activation in lymphomas with MYD88L265P, but not in those without it. Our study revealed a novel mutation-specific biochemical reaction that drives B-cell oncogenesis, providing a therapeutic opportunity for targeting oncogenic MYD88L265P, while sparing MYD88WT, which is critical to innate immunity.
Acute primary repair of extraarticular ligaments and staged surgery in multiple ligament knee injuries
Authors: Ishibashi Yasuyuki , Kimura Yuka , Sasaki Eiji , Sasaki Shizuka , Yamamoto Yuji , Tsuda Eiichi 
Published: October 2020

Abstract
Background
The purpose of this study is to compare the outcomes of acute primary repair of extraarticular ligaments with staged surgery for acute knee dislocations (KDs) and multiligament knee injuries (MLKIs).

Materials and methods
Between January 2005 and May 2018, 61 consecutive patients diagnosed with MLKI or KD were referred to or visited our institution. Of these, 31 patients who underwent acute repair of extraarticular ligaments within 3 weeks of injury were included in this study. These patients were retrospectively classified into two groups: those who underwent only primary repair (repair group) and those who underwent staged reconstructive surgery (staged group). Follow-up examination included range of motion (ROM), knee joint stability (Lachman test, posterior drawer test, and varus and valgus stress test), Lysholm knee score, Tegner activity scale, and Knee Injury and Osteoarthritis and Outcome Score (KOOS).

Results
Twelve of the 31 patients did not need or desire further surgery and were included in the repair group. No significant difference was observed in demographic data between the repair and staged groups. Although staged surgery decreased positive posterior drawer test results, no significant difference was observed between the two groups regarding ROM, other knee joint stability tests, Lysholm scores, Tegner scale, or KOOS.

Conclusions
In this series, all patients returned to their activities of daily living and preinjury occupation levels. Acute primary repair of extraarticular ligaments provides essential knee stability without varus/valgus instability and may reduce the need for subsequent cruciate ligament reconstruction.

Level of evidence
Level IV, retrospective observational study.
Upregulation of miR-361-3p suppresses serotonin-induced proliferation in human pulmonary artery smooth muscle cells by targeting SERT
Authors: Zhang Ying , Chen Yongbin , Chen Guo , Zhou Yingling , Yao Hua , Tan Hong 
Published: October 2020

AbstractBackgroundAbnormal proliferation of pulmonary artery smooth muscle cells (PASMCs) is a key mechanism in pulmonary arterial hypertension (PAH). Serotonin (5-hydroxytryptamine, 5-HT) can induce abnormal proliferation of PASMCs. The role of miR-361-3p in serotonin-induced abnormal PASMCs proliferation remains unclear.MethodsThe miR-361-3p level was analyzed in plasma from PAH patients and normal controls and in human PASMCs (hPASMCs) using RT-PCR. The hPASMCs were transfected with an miR-361-3p mimic and then treated with serotonin. Untransfected hPASMCs were used as the control. Cell proliferation was evaluated using an MTS assay and 5-ethynyl-2′-deoxyuridine (EdU) staining. The cell cycle stages were evaluated using flow cytometry. The association between miR-361-3p and serotonin transporter (SERT) was determined using a luciferase reporter assay and anti-AGO2 RNA immunoprecipitation assay. The protein expression was evaluated via western blotting.ResultsThe miR-361-3p level was lower in plasma from PAH patients than in plasma from the any of the normal control subjects. The mean pulmonary arterial pressure, pulmonary vascular resistance and pulmonary vascular resistance index were higher in PAH patients whose miR-361-3p level was lower than the median value for patients than in those whose miR-361-3p level was higher than the median. Serotonin treatment reduced miR-361-3p expression in the hPASMCs. MiR-361-3p overexpression suppressed cell proliferation, promoted apoptosis, induced G1 arrest, and decreased the phosphorylation level of ERK1/2 in serotonin-treated hPASMCs. SERT was identified as an miR-361-3p target. Its overexpression alleviated the effect of miR-361-3p overexpression on serotonin-induced hPASMC proliferation and upregulation of phosphorylated ERK1/2.ConclusionsThe miR-361-3p level is lower in the plasma of PAH patients. Upregulation of miR-361-3p suppresses serotonin-induced proliferation of hPASMCs by targeting SERT. Our results suggest that miR-361-3p is a potential therapeutic target in PAH.
Effect of asbestos exposure on differentiation and function of cytotoxic T lymphocytes
Authors: Kumagai-Takei Naoko , Nishimura Yasumitsu , Maeda Megumi , Hayashi Hiroaki , Matsuzaki Hidenori , Lee Suni , Yoshitome Kei , Ito Tatsuo , Otsuki Takemi 
Published: October 2020

AbstractAsbestos exposure is known to cause malignant mesothelioma, which is associated with poor prognosis. We focused on and examined the effect of asbestos exposure on the differentiation and function of cytotoxic T lymphocytes (CTLs). CTLs have the ability to specifically attack tumor cells after being differentiated from naïve CD8+ T cells following antigen stimulation. Exposure to chrysotile B asbestos suppressed the differentiation of CTLs during the mixed lymphocyte reaction (MLR) and was associated with a decrease in proliferation of CD8+ T cells. Additionally, in an effort to investigate the mechanism associated with suppressed CTL differentiation upon exposure to asbestos, we focused on IL-2, a cytokine involved in T cell proliferation. Our findings indicated that insufficient levels of IL-2 are not the main cause for the suppressed induction of CTLs by asbestos exposure, although they suggest potential improvement in the suppressed CTL function. Furthermore, the functional properties of peripheral blood CD8+ lymphocytes from asbestos-exposed individuals with pleural plaque (PP) and patients with malignant mesothelioma (MM) were examined. MM patients showed lower perforin levels in CD8+ lymphocytes following stimulation compared with PP-positive individuals. The production capacity of IFN-γ in the MM group tended to be lower compared with healthy volunteers or PP-positive individuals. In an effort to determine whether chronic and direct asbestos exposure affected the function of CD8+ T cells, cultured human CD8+ T cells were employed as an in vitro model and subjected to long-term exposure to chrysotile (CH) asbestos. This resulted in decreased levels of intracellular perforin and secreted IFN-γ. Those findings underlie the possibility that impaired CD8+ lymphocyte function is caused by asbestos exposure, which fail to suppress the development of MM. Our studies therefore reveal novel effects of asbestos exposure on CTLs, which might contribute towards the development and implementation of an effective strategy for the prevention and cure of malignant mesothelioma.
Genome wide association studies for japonica rice resistance to blast in field and controlled conditions
Authors: Volante Andrea , Tondelli Alessandro , Desiderio Francesca , Abbruscato Pamela , Menin Barbara , Biselli Chiara , Casella Laura , Singh Namrata , McCouch Susan R. , Tharreau Didier , Zampieri Elisa , Cattivelli Luigi , Valè Giampiero 
Published: October 2020

AbstractBackgroundRice blast, caused by the fungusPyricularia oryzae, represents the most damaging fungal disease of rice worldwide. Utilization of rice resistant cultivars represents a practical way to control the disease. Most of the rice varieties cultivated in Europe and several other temperate regions are severely depleted of blast resistance genes, making the identification of resistant sources in genetic background adapted to temperate environments a priority. Given these assumptions, a Genome Wide Association Study (GWAS) for rice blast resistance was undertaken using a panel of 311 temperate/tropicaljaponicaandindicaaccessions adapted to temperate conditions and genotyped with 37,423 SNP markers. The panel was evaluated for blast resistance in field, under the pressure of the natural blast population, and in growth chamber, using a mixture of three different fungal strains.ResultsThe parallel screening identified 11 accessions showing high levels of resistance in the two conditions, representing potential donors of resistance sources harbored in rice genotypes adapted to temperate conditions. A general higher resistance level was observed in tropicaljaponicaandindicawith respect to temperatejaponicavarieties. The GWAS identified 14 Marker-Traits Associations (MTAs), 8 of which discovered under field conditions and 6 under growth chamber screening. Three MTAs were identified in both conditions; five MTAs were specifically detected under field conditions while three for the growth chamber inoculation. Comparative analysis of physical/genetic positions of the MTAs showed that most of them were positionally-related with cloned or mapped blast resistance genes or with candidate genes whose functions were compatible for conferring pathogen resistance. However, for three MTAs, indicated as BRF10, BRF11–2 and BRGC11–3, no obvious candidate genes or positional relationships with blast resistance QTLs were identified, raising the possibility that they represent new sources of blast resistance.ConclusionsWe identified 14 MTAs for blast resistance using both field and growth chamber screenings. A total of 11 accessions showing high levels of resistance in both conditions were discovered. Combinations of loci conferring blast resistance were identified in rice accessions adapted to temperate conditions, thus allowing the genetic dissection of affordable resistances present in the panel. The obtained information will provide useful bases for both resistance breeding and further characterization of the highlighted resistance loci.
Optimization of resource demand for passenger services at airports during system failures such as blackouts
Authors: Brause Lisa-Marie , Popa Andrei , Koch Tobias , Deutschmann Andreas , Hellmann Martin 
Published: October 2020

Abstract
Background
In recent years, power outages at airports caused total collapses of terminal operations at the affected airports. These incidents have shown that existing emergency power systems were not sufficient to maintain airport operation. To mitigate the consequences of power outages, it is necessary to improve the management of available resources.

Methodology
Therefore, we investigate the possibility to save resources associated to passenger handling processes before departure. For this purpose, we create a simulation model of a generic medium-sized commercial airport. Several simulations with a varying number of service stations at check-in, security and passport control are carried out. Subsequently, we analyze the number of passengers who do not reach their flight due to increased waiting times at the passenger handling services.

Results
The simulations show that a reduction in resources at check-in counters and passport control initially has no effect on the number of passengers who do not reach their flight. The security control represents a bottleneck in passenger handling. In view of a break-even seat load factor the simulation results show, that a reduction of resources at the check-in below 16 counters, at the security check below 21 and at the passport control below 3 service stations should be avoided.

Conclusion
Therefore, the developed methodology is able to provide airport operators with decision support during system failures, as to which and how many service stations can be dispensed within the sub-processes of passenger handling without interrupting flight operations.
Electrophysiological investigation of the contribution of attention to altered pain inhibition processes in patients with irritable bowel syndrome
Authors: Rustamov Nabi , Wagenaar-Tison Alice , Doyer Elysa , Piché Mathieu 
Published: October 2020

AbstractIrritable bowel syndrome (IBS) is a functional gastrointestinal disorder associated with chronic abdominal pain and altered pain processing. The aim of this study was to examine whether attentional processes contribute to altered pain inhibition processes in patients with IBS. Nine female patients with IBS and nine age-/sex-matched controls were included in a pain inhibition paradigm using counter-stimulation and distraction with electroencephalography. Patients with IBS showed no inhibition of pain-related brain activity by heterotopic noxious counter-stimulation (HNCS) or selective attention. In the control group, HNCS and selective attention decreased the N100, P260 and high-gamma oscillation power. In addition, pain-related high-gamma power in sensorimotor, anterior cingulate and left dorsolateral prefrontal cortex was decreased by HNCS and selective attention in the control group, but not in patients with IBS. These results indicate that the central pain inhibition deficit in IBS reflects interactions between several brain processes related to pain and attention.
MSpectraAI: a powerful platform for deciphering proteome profiling of multi-tumor mass spectrometry data by using deep neural networks
Authors: Wang Shisheng , Zhu Hongwen , Zhou Hu , Cheng Jingqiu , Yang Hao 
Published: October 2020

AbstractBackgroundMass spectrometry (MS) has become a promising analytical technique to acquire proteomics information for the characterization of biological samples. Nevertheless, most studies focus on the final proteins identified through a suite of algorithms by using partial MS spectra to compare with the sequence database, while the pattern recognition and classification of raw mass-spectrometric data remain unresolved.ResultsWe developed an open-source and comprehensive platform, named MSpectraAI, for analyzing large-scale MS data through deep neural networks (DNNs); this system involves spectral-feature swath extraction, classification, and visualization. Moreover, this platform allows users to create their own DNN model by using Keras. To evaluate this tool, we collected the publicly available proteomics datasets of six tumor types (a total of 7,997,805 mass spectra) from the ProteomeXchange consortium and classified the samples based on the spectra profiling. The results suggest that MSpectraAI can distinguish different types of samples based on the fingerprint spectrum and achieve better prediction accuracy in MS1 level (average 0.967).ConclusionThis study deciphers proteome profiling of raw mass spectrometry data and broadens the promising application of the classification and prediction of proteomics data from multi-tumor samples using deep learning methods. MSpectraAI also shows a better performance compared to the other classical machine learning approaches.