bluuebunny/crossref_metadata_2025_split huggingface dataset:
Return to Weightbearing and High-Impact Activities Following Jones Fracture Intramedullary Screw Fixation
Authors: Bucknam Ryan B. , Scanaliato John P. , Kusnezov Nicholas A. , Heida Kenneth A. , Dunn John C. , Orr Justin D. 
Published: January 2020

Background: Although the benefit of primary intramedullary (IM) screw fixation of fifth metatarsal Jones fractures in athletes is clear, limited data support its use in conventional patient populations. This study evaluated radiographic and functional outcomes following primary IM screw fixation in a series of Jones fractures to determine if similar excellent outcomes were achievable. Methods: We reviewed the data of 32 consecutive patients who underwent Jones fracture primary IM screw fixation by a single surgeon. Demographic risk factors of interest (age, gender, tobacco use, pertinent medical comorbidities, military service status, and prior nonoperative management) were collected prospectively. Primary outcomes included times to return to full weightbearing, radiographic union, and resumption of high-impact or restriction-free activities. Complications including reoperations were recorded. Categorical data are reported as frequencies, and statistical means with P values are reported for continuous variables. Mean age for the 32 patients was 33.4 years. Results: All 32 fractures healed uneventfully, and at mean follow-up time of 24.2 months, overall patient-reported satisfaction was 100%. Overall mean postoperative outcomes are as follows: 3.7 weeks return to full weightbearing, 10.8 weeks to radiographic union, and 13.0 weeks to resumption of restriction-free activities. Among the risk factors assessed, only preoperative peripheral vascular disease (PVD) and/or diabetes mellitus (DM) and active duty military service resulted in significantly increased and decreased time to resumption of restriction-free activities, respectively, but did not impact overall weightbearing or union times. Patient age, gender, and tobacco use had no effects on radiographic or functional outcomes. Conclusion: Primary IM screw fixation was a safe, reliable option for all appropriate operative candidates with Jones fractures and may result in similar early weightbearing, osseous healing, and expeditious return to full activities consistently reported in high-level athletes. Level of Evidence: Level IV, case series.
Preliminary Experience With SPECT/CT to Evaluate Periarticular Arthritis Progression and the Relationship With Clinical Outcome Following Ankle Arthrodesis
Authors: DeSutter Christopher , Dube Vincent , Ross Andrew , Boyd Gordon , Morash Joel , Glazebrook Mark 
Published: January 2020

Background: Single photon emission computed tomography combined with computed tomography (SPECT/CT) could potentially aid in diagnosing periarticular arthritis/activity and progression, facilitate effective treatment options, and evaluate the effect surgery has on the clinical outcome of patients with ankle arthritis. The goal of our study was to assess SPECT/CT activity in the ankle and periarticular joints before and after ankle fusion surgery and determine whether it was associated with clinical pain and function scores. Methods: Thirty-four patients recruited into this study underwent either arthroscopic or open ankle fusion. X-ray (XR) and SPECT/CT imaging was obtained as well as completion of patient Ankle Osteoarthritis Scale (AOS) and 36-item Short Form Survey (SF-36) questionnaires preoperatively and at 6 months postoperatively. Ankle, subtalar, and talonavicular joint arthritis grading on XR and CT, along with SPECT/CT activity, was evaluated by 2 nuclear medicine radiologists. Data were assessed for normality and analyzed with the appropriate comparative test. P value was set at <.05. Thirty patients (31 ankles) completed follow-up and were analyzed. Results: SPECT/CT activity showed no significant difference in the ankle joint at 6 months postoperatively while periarticular joint activity significantly increased ( P < .05). Six months postoperatively, patients had significant improvements in their AOS and SF-36 scores ( P < .05). SPECT/CT grading of all joints analyzed, however, was not associated with AOS or SF-36 scores preoperatively or at 6 months postoperatively. Conclusion: In this study, intensity of activity as evaluated by SPECT/CT in periarticular hindfoot joints in patients who had ankle arthrodesis was not associated with clinical/functional scores at 6 months postoperatively. Level of Evidence: Level IV, diagnostic study.
A case of mitoxantrone extravasation
Authors: Chang Abraham 
Published: January 2020

Introduction Mitoxantrone is a chemotherapeutic agent approved for various diseases. The literature has been conflicting in classifying mitoxantrone as a vesicant or irritant. Case report We report a patient who had an extravasation of mitoxantrone. Mitoxantrone was administered in 50 ml normal saline. After mitoxantrone was completely infused, the site appeared edematous and the blue color of mitoxantrone developed beneath the skin. The patient reported pain. Management and outcome: The extravasation was treated with dexrazoxane and cold compresses. The pain improved each day. However, blistering developed five weeks later and the patient ultimately required surgical intervention for debridement and grafting. Discussion Extravasation events are rare and there are few controlled studies. Because of the similarities in chemical structures and mechanism of actions between mitoxantrone and anthracyclines, mitoxantrone extravasation is often treated similar to anthracyclines. Mitoxantrone's classification is unclear, as some literature classifies it as a vesicant and others as an irritant. Our case supports the categorization of mitoxantrone as a vesicant.
Risk factors for ocular complications in adult patients with uveitis
Authors: Prieto-del-Cura Mar , González-Guijarro Juan Jacobo 
Published: January 2020

Purpose: To report risk factors for ocular complications in adults with uveitis. Methods: Retrospective cohort study of 653 patients with uveitis (869 eyes) followed up for at least 1 month attended between May 1989 and December 2012. Results: After a median follow-up of 16 months (interquartile range = 3–59), 43.5% of patients developed one or more complications, including cataract formation (25%), iris alterations (20%), and macular edema (16%) as the most frequent. Older age, intermediate uveitis, chronic course, and uveitis activity >3 months were significantly more frequent among patients with complications. In the multivariate analysis, age ⩾65 years (hazard ratio = 2.76, 95% confidence interval = 1.72–4.4), chronic course (hazard ratio = 1.78, 95% confidence interval = 1.23–259), and uveitis activity >3 months (hazard ratio = 1.94, 95% confidence interval = 1.33–2.84) were predictors of complications. Conclusion: In the present series of patients with uveitis, advanced age, chronic uveitis, and inflammatory activity >12 weeks were risk factors for complications.
Endoscopic Radial Artery Harvesting During Anesthesia Line Placement Reduces the Time and Cost of Multivessel Coronary Artery Bypass Grafting
Authors: Wang Hanjay , Bilbao Mary S. , Miller Shari L. , O’Donnell Christian T. , Boyd Jack H. 
Published: January 2020

Objective Endoscopic radial artery (RA) harvesting performed concurrently with internal mammary artery (IMA) takedown and endoscopic saphenous vein (SV) harvesting creates a crowded and inefficient operating room environment. We assessed the effect of a presternotomy RA harvest strategy on surgery time and costs. Methods A total of 41 patients underwent elective, first-time, isolated multivessel on-pump coronary artery bypass grafting including an IMA, RA, and SV graft. The first 20 patients (Phase I) underwent endoscopic RA harvesting concurrently with IMA takedown and endoscopic SV harvesting after sternotomy, requiring two sets of endoscopic harvesting equipment per case, each used by a separate individual. The final 21 patients (Phase II) underwent endoscopic RA harvesting during anesthesia line placement, completing the procedure before sternotomy, thus requiring only one set of endoscopic harvesting equipment reused by a single individual. Results There were no differences in baseline patient characteristics, number of bypasses, duration of SV or RA harvest time, or duration of cardiopulmonary bypass or cross-clamp time between the two groups. Total surgery time was reduced by 32 minutes in Phase II ( P = 0.044). Relative to a total hospital direct cost of 100.00 units, total surgery costs were reduced from 29.33 units in Phase I to 25.62 units in Phase II ( P = 0.001). No anesthesia- or RA harvest-related complications occurred in either group. Conclusions Endoscopic RA harvesting can be safely performed during anesthesia line placement prior to sternotomy. Our simple but innovative strategy improves intraoperative workflow, reduces the time and cost of surgery, and advances the delivery of high-quality patient care.
Complete Sternal-Sparing Approach Improves Outcomes for Left Ventricular Assist Device Implantation in Patients With History of Prior Sternotomy
Authors: Ayers Brian , Sagebin Fabio , Wood Katherine , Barrus Bryan , Thomas Sabu , Storozynsky Eugene , Chen Leway , Bernstein Wendy , Lebow Brandon , Prasad Sunil , Gosev Igor 
Published: January 2020

Objective Early reports of less invasive techniques for left ventricular assist device (LVAD) implantation have demonstrated promising results. We sought to investigate the safety and feasibility of implementing the complete sternal-sparing (CSS) approach for LVAD implantation in patients with a history of prior cardiac operation. Methods This was a retrospective review of prospectively collected data for all patients implanted with a fully magnetically levitated LVAD from April 2017 through December 2018. Patients were dichotomized based on surgical approach: CSS or full median sternotomy (FS). Perioperative complications and overall survival were compared between cohorts. Results Of the 29 eligible patients, 15 (52%) were implanted via the CSS approach and 14 (48%) via FS. Preoperative characteristics were similar between cohorts. Overall survival to discharge was 93% for CSS compared to 71% for FS ( P = 0.169). The CSS cohort demonstrated fewer postoperative complications, including fewer cases of severe right ventricular failure ( P = 0.006) and less blood product utilization ( P = 0.015). Median hospital length of stay was significantly shorter for the CSS cohort (median 13 vs 32.5 days, P = 0.016). Neither cohort had any 30-day readmissions. Conclusions Early data suggest that the CSS technique is a safe and effective technique for patients with a history of prior sternotomy. Further studies are needed to validate this single-center experience.
Flat-panel detector CT assessment in stroke to reduce times to intra-arterial treatment: A study of multiphase computed tomography angiography in the angiography suite to bypass conventional imaging
Authors: Bouslama Mehdi , Haussen Diogo C , Grossberg Jonathan A , Barreira Clara M , Bom Imramsjah Martijn J van der , Nijnatten Fred van , Grünhagen Thijs , Moyer Larry , Frankel Michael R , Nogueira Raul G 
Published: January 2020

Background Bypassing the emergency department and the computed tomography suite by directly transporting to the neuroangiography suite for imaging assessment and treatment may shorten reperfusion times while maintaining proper patient selection. Objective To determine whether flat-panel detector multiphase computed tomography angiography protocol is associated with reduced treatment times and a similar safety profile as the standard imaging protocol. Methods Single-center prospective study of consecutive patients with anterior circulation large vessel occlusion strokes transferred to our facility for consideration of endovascular therapy from May 2016 to December 2017. Those with basilar strokes and/or presenting to the emergency department were excluded. Patients were categorized into two groups: (1) flat-panel detector CT assessment in stroke to reduce times to intra-arterial treatment group, with patients transferred directly to the suite for multiphase computed tomography angiography; and (2) patients undergoing standard protocol including computed tomography ± computed tomography angiography/CT perfusion. The groups were matched for age, baseline National Institute of Health Stroke Scale, and pretreatment glucose. Baseline characteristics, time metrics, and outcomes were compared. Results Out of 419 patients who underwent endovascular therapy over the study period, 210 patients fit inclusion criteria, with 54 (25.7%) in the flat-panel detector CT assessment in stroke to reduce times to intra-arterial treatment group. After matching, 49 flat-panel detector CT assessment in stroke to reduce times to intra-arterial treatment/control pairs were generated and analyzed. Baseline characteristics were well balanced. Flat-panel detector CT assessment in stroke to reduce times to intra-arterial treatment patients had significantly shorter median door-to-puncture (33 [26.5-47] vs. 55 [44.5–66] min, p < 0.001), door-to-reperfusion (85 [57.5–115.5] vs. 110 [80–153], p = 0.005) and picture-to-puncture (18 [13.5–22.5] vs. 42 [32–47.5] min, p < 0.001) times. There were no differences in rates of successful reperfusion (modified thrombolysis in cerebral infarction 2b-3, 95.9% vs. 100%, p = 0.5), parenchymal hematomas type-2 (4.1% vs. 2%, p = 1.00), good outcome (90-day modified Rankin Scale 0–2, 44.9% vs. 40.8%, p = 0.68), and 90-day mortality (14.3% vs. 22.4%, p = 0.30). Conclusion Directly transferring patients to angiography and using multiphase computed tomography angiography to determine the eligibility for endovascular therapy is safe and may result in a significant reduction in treatment times. Future larger studies are warranted.
Return to Driving After Foot and Ankle Surgery: A Novel Survey to Predict Passing Brake Reaction Time
Authors: McDonald Elizabeth L. , Shakked Rachel , Nicholson Kristen , Daniel Joseph N. , Pedowitz David I. , Winters Brian S. , Rogero Ryan , Raikin Steven M. 
Published: January 2020

Introduction. Brake reaction time (BRT) is an accepted method for establishing recommendations for safe return to driving by the National Highway Traffic Safety Administration. Other than performing a BRT test in clinic, there is no established clinical tool to help physicians differentiate safe from unsafe drivers once patients reach general recovery milestones. The purpose is to present individual recommendations to the patient through a novel, validated survey evaluating safe return to driving after orthopaedic surgery of the right foot and ankle. Methods. A total of 171 patients undergoing 1 of 3 specific foot and ankle procedures were prospectively enrolled. A 4-question survey and BRT were completed 6 weeks postoperatively. The following questions were asked: (1) “I think my brake reaction time is slower than most drivers my age,” (2) “I think my brake reaction time is faster than most drivers my age,” (3) “I think my brake reaction time is about the same as most drivers my age,” (4) “Based on what I think my brake reaction time is, I think I am ready to drive.” Internal consistency was determined with Cronbach’s α and item total correlation. External validity was determined by Spearman’s correlation coefficient. A BRT less than 0.850 s was considered as a pass. Results. Of 171 patients, 162 (95%) with ages ranging from 21 to 83 years achieved a passing BRT by 7.6 weeks. After removing 1 question because of internal inconsistency, the optimal threshold for predicting passing BRT was 10/15 points or higher, which had 99% probability of success that a patient would pass the BRT (95% CI = 96%, 100%). Conclusion. This novel, 3-question driving readiness survey can accurately predict a passing BRT Achilles rupture repair, total ankle arthroplasty, and hallux valgus correction performed in the right foot and ankle as early as 6 weeks postoperatively. Level of Evidence: Level II: Comparative study
Relationship between agricultural pesticides and the diet of riparian spiders in the field
Authors: Graf Nadin , Battes Karina P. , Cimpean Mirela , Entling Martin H. , Frisch Katharina , Link Moritz , Scharmüller Andreas , Schreiner Verena C. , Szöcs Eduard , Zubrod Jochen P. , Schäfer Ralf B. 
Published: January 2020

Abstract
Background
Examining the effects of pesticides is difficult in regions such as Western Europe because of the relatively ubiquitous use of agrochemicals and the lack of unaffected areas. To study a wide gradient of agrochemical stress, we conducted a study in Central Romania, where traditional agriculture, which is assumed to use less agrochemicals, exists adjacent to intensive agriculture. We investigated potential effects of land use related stressors including pesticides on aquatic–terrestrial predator–prey relationships using stable isotope analysis. Therefore, we sampled spiders, as well as their aquatic and terrestrial prey along streams with a pesticide pollution gradient and determined spider and prey stable carbon and nitrogen signals.

Results
Aquatic prey contributed 40.8–55.4% to the diet of the orb web weaving spider Tetragnatha sp. and 34.0–53.0% to the diet of the ground-hunting Pardosa sp. The biomass of potential aquatic prey increased along a gradient of increasing riparian habitat conditions and water quality and decreasing agriculture (e.g. arable land) in the catchment. The proportion of aquatic prey in the orb web weavers diet responded positively to the biomass of potential aquatic prey and negatively to this gradient. Increasing potential prey biomass resulted in an increasing proportion of aquatic prey for the orb web weaver. The proportion of aquatic prey in the ground hunters diet increased with in-stream pesticide toxicity and along a gradient of increasing pastoralism in the catchment as well as increasing riparian habitat and water quality.

Conclusion
The diet of two riparian predators responded to complex gradients of catchment land use, stream and riparian habitat quality as well as to in-stream pesticide toxicity. The responses to the different environmental variables can be attributed to direct (e.g. change in spider community) and indirect (e.g. changes in aquatic prey and terrestrial prey) effects. Future manipulative field studies or experiments on aquatic–terrestrial food webs are required to examine the causality of our findings and should also consider the quality of prey organisms to foster mechanistic understanding of cross-ecosystem effects.
Comparative analysis on the expression of L1 loci using various RNA-Seq preparations
Authors: Kaul Tiffany , Morales Maria E. , Sartor Alton O. , Belancio Victoria P. , Deininger Prescott 
Published: January 2020

Abstract
Background
Retrotransposons are one of the oldest evolutionary forces shaping mammalian genomes, with the ability to mobilize from one genomic location to another. This mobilization is also a significant factor in human disease. The only autonomous human retroelement, L1, has propagated to make up 17% of the human genome, accumulating over 500,000 copies. The majority of these loci are truncated or defective with only a few reported to remain capable of retrotransposition. We have previously published a strand-specific RNA-Seq bioinformatics approach to stringently identify at the locus-specific level the few expressed full-length L1s using cytoplasmic RNA. With growing repositories of RNA-Seq data, there is potential to mine these datasets to identify and study expressed L1s at single-locus resolution, although many datasets are not strand-specific or not generated from cytoplasmic RNA.

Results
We developed whole-cell, cytoplasmic and nuclear RNA-Seq datasets from 22Rv1 prostate cancer cells to test the influence of different preparations on the quality and effort needed to measure L1 expression. We found that there was minimal data loss in the identification of full-length expressed L1 s using whole cell, strand-specific RNA-Seq data compared to cytoplasmic, strand-specific RNA-Seq data. However, this was only possible with an increased amount of manual curation of the bioinformatics output to eliminate increased background. About half of the data was lost when the sequenced datasets were non-strand specific.

Conclusions
The results of these studies demonstrate that with rigorous manual curation the utilization of stranded RNA-Seq datasets allow identification of expressed L1 loci from either cytoplasmic or whole-cell RNA-Seq datasets.
Isolation and characterization of pathogenic Escherichia coli bacteriophages from chicken and beef offal
Authors: Lukman Celosia , Yonathan Christopher , Magdalena Stella , Waturangi Diana Elizabeth 
Published: January 2020

AbstractObjectiveThis study was conducted to isolate and characterize lytic bacteriophages for pathogenicEscherichia colifrom chicken and beef offal, and analyze their capability as biocontrol for several foodborne pathogens. Methods done in this research are bacteriophage isolation, purification, titer determination, application, determination of host range and minimum multiplicity of infection (miMOI), and bacteriophage morphology.ResultsSix bacteriophages successfully isolated from chicken and beef offal using EPEC and EHEC as host strain. Bacteriophage titers observed between 109and 1010PFU mL−1. CS EPEC and BL EHEC bacteriophage showed high efficiency in reduction of EPEC or EHEC contamination in meat about 99.20% and 99.04%. The lowest miMOI was 0.01 showed by CS EPEC bacteriophage. CI EPEC and BL EPEC bacteriophage suspected asMyoviridaefamily based on its micrograph from Transmission Electron Microscopy (TEM). Refers to their activity, bacteriophages isolated in this study have a great potential to be used as biocontrol against several foodborne pathogens.
Draft genome sequence of the strain 16-537536, isolated from a patient with bronchiectasis and its relationship to the Pseudomonas koreensis group of the Pseudomonas fluorescens complex
Authors: Fluit Ad. C. , Rogers Malbert R. C. , Díez-Aguilar María , Cantón Rafael , Benaissa-Trouw Barry J. , Bayjanov Jumamurat R. , Ekkelenkamp Miquel B. 
Published: January 2020

Abstract
Objective
The Pseudomonas koreensis group bacteria are usually found in soil and are associated with plants. Currently they are poorly described. Here we report on the whole genome sequence of a bacterial isolate from a patient with bronchiectasis that was first identified as P. koreensis, and on its position in the P. koreensis group.

Results
Strain 16-537536 was isolated from a patient with bronchiectasis from Spain and initially identified by MALDI-TOF as P. koreensis, a member of the Pseudomonas fluorescens complex. However, the average nucleotide identity analysis (ANIb) and whole genome alignments of the draft genome sequence of this strain showed it to be a member of the P. koreensis group of the P. fluorescens complex, but belonging to an undescribed species. In addition, based on ANIb analysis, the P. koreensis group contains several other unnamed species. Several genes for putative virulence factors were identified. The only antibiotic resistance gene present in strain 16-537536 was a class C β-lactamase. The correct identification of bacterial species from patients is of utmost importance in order to understand their pathogenesis and to track the potential spread of pathogens between patients. Whole genome sequence data should be included for the description of new species.