bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of differentially expressed circulating exosomal lncRNAs in IgA nephropathy patients
Authors: Guo Na , Zhou Qin , Huang Xiang , Yu Jianwen , Han Qianqian , Nong Baoting , Xiong Yuanyan , Liang Peifeng , Li Jiajia , Feng Min , Lv Jun , Yang Qiongqiong 
Published: March 2020

Abstract
Background: Immunoglobulin A nephropathy (IgAN) is one of the most prevalent primary glomerular disease. Non-invasive biomarkers are urgently needed for IgAN diagnosis. We investigate the difference in expression profiles of exosomal long non-coding-RNAs (lncRNAs) in plasma from IgAN patients compared with their healthy first-degree relatives, which may reveal novel non-invasive IgAN biomarkers.
Methods: Exosomes were isolated from the plasma of IgAN patients and their healthy first-degree relatives. High-throughput RNA sequencing and real-time quantitative polymerase chain reaction (qRT-PCR) was used to validate lncRNA expression profiles. Pathway enrichment analysis was used to predict their nearest protein-coding genes.
Results: lncRNA-G21551 was significantly down-regulated in IgAN patients. The nearest protein-coding gene was found to be encoding the low affinity receptor of the Fc segment of immunoglobulin G (FCGR3B).
Conclusion: Exosomal lncRNA-G21551, with FCGR3B as the nearest protein-coding gene, was down-regulated in IgAN patients, indicating its potential to serve as a non-invasive biomarker for IgAN.
Effectiveness of recombinant Escherichia coli on the production of (R)-(+)-perillyl alcohol
Authors: Sun Chao , Dong Xianjuan , Zhang Rubing , Xie Congxia 
Published: March 2020

Abstract
Background : ( R )-(+)-perillyl alcohol is a naturally oxygenated monoterpene that is produced in perilla leaves, citrus, lemon and lavender. Perillyl alcohols have many uses, including as natural flavor additives, insecticides, jet fuels and anti-cancer therapies. The bioconversion of readily available monoterpene precursors, such as ( R )-(+)-limonene, are recognized as valuable oxygenated derivatives. However, as this natural product is present at low concentrations in plant oils, alternative microbial production methods are required for its extraction from natural plant sources.
Results : We engineered Escherichia coli to possess a heterologous mevalonate (MVA) pathway, including limonene synthase , P-cymene monoxygenase hydroxylase and P-cymene monoxygenase reductase for the production of ( R )-(+)-perillyl alcohol. The concentration of ( R )-(+)-limonene (the monoterpene precursor to ( R )-(+)-perillyl alcohol) reached 27.3 mg/L from glucose. Enhanced ( R )-(+)-perillyl alcohol production was therefore achieved. The strain produced ( R )-(+)-perillyl alcohol at a titer of 45.7 mg/L in a 5 L bioreactor fed batch system.
Conclusions : These data highlight the efficient production of ( R )-(+)-perillyl alcohol through the mevalonate pathway from glucose. This method serves as a platform for the future production of other monoterpenes.
Assembly and comparative analysis of chloroplast genome of wheat K-CMS line and maintainer line
Authors: Han Yucui , Gao Yujie , Zhai Xiaoguang , Zhou Hao , Ding Qin , Ma Lingjian 
Published: March 2020

Abstract
The sterile line is vital for the heterosis utilization of wheat (Triticum aestivum L.), and the cytoplasmic male sterility (CMS) line has more practical significance in the utilization of heterosis. To reveal the sterile mechanism of wheat K-CMS line K519A from the perspective of the chloroplast genome, the chloroplast genomes of the K519A and its same nuclear genotype maintainer line 519B were sequenced using second-generation high-throughput technology and assembled. Then the chloroplast genomic contents, SSR sequences, long repeat fragments, and qPCR were analyzed. The results showed that the total length of K519A and 519B were 136,996 bp and 136,235 bp, respectively. Both chloroplast genomes encode 126 genes, including 89 protein-coding genes, 8 rRNA genes, and 39 tRNA genes. There were 186 and 188 SSRs in K519A and 519B, respectively. And a developed SSR maker, which from atpF, can be used to distinguish the cytoplasmic type of Aegilops kotschyi and Triticum aestivum, respectively. There were 50 and 52 long repeat fragments, which come from the gene sequences, in K519A and 519B, respectively. A total of 107 mutations between K519A and 519B, which from 45 genes, were identified, of which, 16 genes (matK, rps16, rpoB, rpoC2, atpI, ndhK, atpB, rbcL, psbB, psbH, rpl14, ndhH, ndhF, rpl32, ccsA, ndhA) contained non-synonymous mutations. Further, the qPCR analysis was performed, and the gene expression levels of selected six genes for K519A compared to 519B were mostly downregulated at the binucleate and trinucleate stages of pollen development, thus, these non-synonymous mutant genes might affect the fertility of K519A.
Taxonomy, diversity and diagnosis of Tetrahymenosis, and its recent identification measures in aquaculture inferred from infections in Northeastern China
Authors: Pan Xuming , Zhang shuixian , Pan mengmeng , Li Wenyu , Sun meng , Wang chongnv , Liang Jiale , Wang xiang , Zhu lei , Chen Ying 
Published: March 2020

Abstract
Background Tetrahymenosis caused by about ten parasitic Tetrahymena species belonging to the Phylum Ciliophora has been recognized as an emerging problem inflicting significant economic loss in aquaculture industry in the world. Increasing knowledge and identification of Tetrahymenosis are important. Methods Four parasitic Tetrahymena species were collected from eight commercially farmed fishes in Harbin, northeastern China. Specific oligonucleotide probe and fluorescence in situ hybridization staining was designed and tested. Hoechst33342 staining methods, gene sequencing and phylogenetic analyses were also conducted. Results Tetrahymena pyriformis, T. vorax, T. chironomi and T. bergeri are the four species responsible for Tetrahymenosis in various fishes in Harbin. Taxonomy, diagnosis, diversity, pathogenicity, and histopathology of Tetrahymenosis were supplemented, analyzed and summaried, based on the present and previous work. The term Tetrahymenosis is diagnosised as diseases affecting a number of fishes, crustaceans, mollus, beetle, dragonfly, salmon, slug, midge larvae and freshwater mussel species that caused by ciliates of the genus Tetrahymena which constitutes an abundant group inhabit various aquaculture and natural habitats. Improved classification of parasitic Tetrahymena was provided. Phylogeny of parasitic Tetrahymena species was studied, and an SSU-rDNA targeted oligonucleotide probe labeled with a fluorochrome was designed, and the FISH protocol was optimized for identification of Tetrahymena species, Conclusions The manifestations of histopathology in fish typically include lesions on the body surface, and affected organs include the skin, musculature, viscera, eye socket and spinal cord; masses of ciliates can be detected in copious amount of mucus and between spaces in the damaged tissues. Improved classification of parasitic Tetrahymena is provided: facultatively parasitic forms ( T. pyriformis , T. rostrate , T. bergeri and T. vorax ), facultatively free-living forms ( T. chironomi , T. corlissi, T. rotunda, T. glochidiophila and T. papula ) and parasitic forms ( T. stegomyiae and T. limacis ).The phylogenic results indicate that Tetrahymena spp. belonging to ‘borealis’ group have a greater probability to become parasitism. the method of which can be used for quick and early detection of Tetrahymenosis.
Bone ongrowth and mechanical fixation of implants in cortical and cancellous bone
Authors: Walsh William Robert , Pelletier Matthew Henry , Bertollo Nicky , Lovric Vedran , Wang Tian , Morberg Per , Parr William Chase Harington , Bergadano Dario 
Published: March 2020

Abstract
Background What is the right surface for an implant to achieve biological fixation? Surface technologies can play important roles in encouraging interactions between the implant surface and the host bone to achieve osseointegration. Pre-clinical animal models provide important insight into in-vivo performance related to bone ongrowth and implant fixation. Methods A large animal model was used to compared the in vivo response of HA and plasma sprayed titanium coatings in a well reported adult ovine model to evaluate bone ongrowth in terms of mechanical properties in cortical sites, histology and histomorphometry in cortical and cancellous sites at 4 and 12 weeks. Results Titanium plasma sprayed surfaces outperformed the HA coated samples in push-out testing in cortical sites while both surfaces supported new bone ongrowth and remodeling in cortical and cancellous sites. Conclusions While both HA and Ti plasma provided an osteoconductive surface for bone ongrowth, the Ti plasma provided a more robust bone-implant interface that ideally would be required for load transfer and implant stability in the longer term.
The 100 most influential studies in CAR-T: a bibliometric analysis
Authors: Seo Beomjun , Kim Jeeyoon , Kim Seungwook 
Published: March 2020

Abstract
Background: Bibliometric analysis of mainly cited articles is used to provide information on trends in a specific research field and objective indicators of the scientific impact of the publication. With bibliometric and network analysis, we map the scientific landscape of chimeric antigen receptors T-cells (CAR-T) research. Methods: Extract 100 most cited articles published over the last decade (from January 1, 2009 to Dec 31, 2018; 10 years) from the Web of Science Core Collection with bibliographic details; year of publication, country of author, funding agencies, research organization, author information, and keywords. Results: Of the 100 papers identified, most (92%) were written in the US. US government agencies and non-profit organizations provided the most funding, and the papers funded by the NIH had the most citations, followed by those funded by the Alliance for Cancer Gene Therapy (US). 33 papers out of the top 100 most cited papers were published from the University of Pennsylvania. As for authors, Carl H. June participated in 30 papers, followed by Bruce L. Levine who participated in 11 papers. As for journals, Blood (n=20), published the most papers, followed by Science Translational Medicine (n=10). The most frequently used keyword was “adoptive immunotherapy” (n=37), followed by “lymphocytes” (n=27), and “antitumor-activity” (n=25). Conclusion: We performed the quantitative bibliometric analysis of funding bodies, countries, organizations, journals, authors, and keywords for the CAR-T research trends and landscape. Moving forward, Analysis of highly influential CAR-T articles provides insight into areas for future development.
Evaluation of the causal effects of blood lipid levels on gout with summary level GWAS data: two-sample Mendelian randomization and mediation analysis
Authors: Yu Xinghao , Wang Ting , Huang Shuiping , Zeng Ping 
Published: March 2020

Abstract
Background: Observational studies have identified gout patients are often comorbid with dyslipidemia, which is characterized by a decrease in high-density lipoprotein cholesterol (HDL) and an increase in triglycerides (TG). However, the relationship between dyslipidemia and gout is still unclear. Methods: We first performed Mendelian randomization (MR) to evaluate the causal effect of four lipid traits on gout and serum urate based on publicly available GWAS summary statistics ( n ~100,000 for lipid, 69,374 for gout and 110,347 for serum urate). We then employed multivariable MR to estimate the independent causal effect for lipid on gout via serum urate with mediation analysis. Results: MR showed each standard deviation (SD) (~12.26 mg/dL) increase in HDL resulted in about 25% (95% CI 9.0% ~ 38%, p = 3.31E-3) reduction of gout risk, with 0.09 mg/dL (95% CI: -0.12 ~ -0.05, p = 7.00E-04) decrease in serum urate, and each SD (~112.33 mg/dL) increase of TG was associated with 0.10 mg/dL (95% CI: 0.06 ~ 0.14, p = 9.87E-05) increase in serum urate. Those results were robust against various sensitive analyses. Additionally, independent effects of HDL and TG on gout/serum urate were confirmed. Finally, mediation analysis demonstrated HDL or TG could also indirectly affect gout via the pathway of serum urate. Conclusion: Our study confirmed the causal associations between HDL (and TG) and gout, and further revealed the effect of HDL or TG on gout could also be mediated via serum urate.
A multicenter study assessing the prevalence of germline genetic alterations in Chinese gastric cancer patients
Authors: Zhang Yinjie , Yang Yang , Wei Qing , Xu Ting , Zhang Xiaotian , Gao Jing , Zhu Changbin , Shao Di , Ke Yang , Tan Siyi , Liu Baorui , Zhang Jingdong , Chen Xiaobing , Wang Zhaojie , Qiu Meng , Wang Xin , Shen Lin , Wang Xicheng 
Published: March 2020

Abstract
Background: Approximately 10% of patients with gastric cancer (GC) have a genetic predisposition for the disease. To date, knowledge regarding germline mutations in predisposing genes in the Chinese GC population is scarce. The aim of this study was to determine the spectrum and distribution of predisposing gene mutations among Chinese GC patients known to have hereditary high-risk factors for cancer. Methods: Forty patients from among ten families were recruited from seven medical institutions in China. Next-generation sequencing was performed on 171 genes associated with cancer predisposition. For probands with pathogenic/likely pathogenic germline variants, Sanger sequencing was used to validate the variants in the probands and their relatives. Results: Sequencing indicated that 25% (10/40) of the patients carried a combined total of ten pathogenic or likely pathogenic germline variants involving nine different genes: CDH1 (n = 1), MLH1 (n = 1), MSH2 (n = 1), CHEK2 (n = 1), BLM (n = 1), EXT2 (n = 1), PALB2 (n = 1), ERCC2 (n = 1), and SPINK1 (n = 2). Five of these variants have not previously been reported. In addition, a total of 129 variants of uncertain significance were identified in 27 patients. Conclusions: This study found that 25% of Chinese GC patients with hereditary high-risk factors have deleterious germline alterations. This result may indicate a unique genetic background of GC among Chinese patients.
Presence of additional P. vivax malaria in Duffy negative individuals from Southwestern Nigeria: Short Report
Authors: OBOH MARY Aigbiremo , Singh Upasana Shyamsunder , Ndiaye Daouda , Badiane Aida Sadikh , Ali Nazia Anwar , Bharti Praveen Kumar , Das Aparup 
Published: March 2020

Abstract
Background Malaria in sub-Saharan Africa (sSA) is thought to be hugely caused by Plasmodium falciparum . Recently, growing reports of cases due to P. ovale , P. malariae , and P. vivax have been significantly reported to play a role in malaria epidemiology in sSA. This in fact is due to the usage of very sensitive diagnostic tools (e.g. PCR) which have highlighted the underestimation of non-falciparum malaria in this sub-region. P. vivax was historically thought to be absent in sSA due to the high prevalence of the Duffy null antigen in individuals residing in this sub-continent. For example, recent studies reporting the detection of vivax malaria in Duffy-negative individuals from Mali, Mauritania, Cameroon to mention a few challenges this notion.Methods Following our earlier report of P. vivax in Duffy-negative individuals, we have collected and assessed RDT and/or microscope malaria positive samples following the conventional PCR method and DNA sequencing to confirm both single/mixed infections as well as the Duffy status of the individuals.Results Amplification of Plasmodium gDNA was possible in 59.9% (145/242) of the evaluated isolates and as expected P. falciparum was the most predominant (91.7%) species identified. Interestingly, four P. vivax isolates were identified either as single (3) or mixed (1 – P. falciparum / P. vivax ) infection. Sequencing results confirmed, all vivax isolates as truly vivax malaria and their Duffy status to be that of the Duffy-negative genotype.Conclusion Identification of more vivax isolates among these Duffy-negative individuals from Nigeria, substantiate the expanding body of evidence on the ability of P. vivax to infect RBCs that do not express the DARC gene. Hence, such genetic-epidemiological study should be conducted at the national level in order to evaluate the actual burden of P. vivax in the country.
Moral Competence, Moral Teamwork and Moral Action – The European Moral Case Deliberation Outcomes (Euro-MCD) Instrument 2.0 and its revision process
Authors: de Snoo-Trimp Janine , de Vet Riekie , Widdershoven Guy , Molewijk Bert , Svantesson Mia 
Published: March 2020

Abstract
Background Clinical Ethics Support (CES) services are offered to support healthcare professionals in dealing with ethically difficult situations. Evaluation of CES is important to understand if it is indeed a supportive service in order to inform and improve future implementation of CES. Yet, methods to measure outcomes of CES are scarce. In 2014, the European Moral Case Deliberation Outcomes Instrument (Euro-MCD) was developed to measure outcomes of Moral Case Deliberation (MCD). To further validate the instrument, we tested it in field studies and revised it. This paper presents the Euro-MCD 2.0 and describes the revision process.Methods The revision process comprised an iterative dialogue among the authors as Euro-MCD-project team, including empirical findings from six Euro-MCD field-studies and input from European experts in CES and theory. Empirical findings contained perceptions and experiences of MCD outcomes among healthcare professionals who participated in MCDs in various settings in Norway, Sweden and the Netherlands. Theoretical viewpoints on CES, literature on goals of CES and MCD and ethics theory guided the interpretation of the empirical findings and final selection of MCD outcomes.Results The Euro-MCD 2.0 Instrument includes three domains: Moral Competence, Moral Teamwork and Moral Action. Moral Competence consists of items about moral sensitivity, analytical skills and virtuous attitude. Moral Teamwork includes open dialogue and supportive relationships and Moral Action refers to moral decision-making and responsible care. During the revision process, we made decisions about adding and reformulating items as well as decreasing the number from 26 to 15 items. We also altered the sentence structure of items to assess the current status of outcomes (e.g. ‘now’) instead of an assumed improvement over time (e.g. ‘better’) and we omitted the question about perceived importance.Conclusions : The Euro-MCD 2.0 is shorter, less complex and more strongly substantiated by an integration of empirical findings, theoretical reflections and dialogues with participants and experts. Use of the Euro-MCD 2.0 will facilitate evaluation of MCD and can thereby monitor and foster implementation and quality of MCD. The Euro-MCD 2.0 will strengthen future research on evaluation of outcomes of MCD.
Multiple factors involved in T cell depletion in AIDS patients with immune non-responder to cART
Authors: Qian Feng , Hu Song , Zhu Yueping , Wang Yinling , Liu Jin , Qiao Jialu , Shu Xiji , Gao Yong , Sun Binlian , Zhu Chuanwu 
Published: March 2020

Abstract
Background: The replication of HIV-1 can be effectively controlled by combination antiretroviral therapy (cART) at present. However, in approximately 20% of AIDS patients with undetectable viral load under effective cART, the amount of CD4+ T cells continues to be lower than normal, showing poor immune reconstitution, which is called immune non-responder (INR). The mechanism of immune reconstitution in AIDS patients is still unclear, and thus far there is no effective improvement strategy. Aim: To investigate what factors involved in T lymphocyte depletion in INR patients.Methods: A retrospective analysis of a cohort from Suzhou Infectious Disease Hospital was performed. From October 2015 to April 2019, 29 AIDS patients, whose CD4+ T cells were less than 100/ul before treatment, were included in this study. Laboratory results were obtained from the medical records, including basic clinical information, and the results of Flow cytometer detection for CD4 T cells and ELISA for T cell-related cytokines.Results: According to the number of CD4+ T cells after 24 months of treatment, the 29 patients were divided into two groups: 15 patients with CD4+ T cells <200/ul were INR, and 14 patients with CD4+ T cells >200/ul were immune responder (IR). When Compared with IR patients, the total number of red blood cells and white blood cells, especially T lymphocytes in INR patients, was significantly lower, while programmed cell death protein 1（PD-1）on CD4+T and CD8+ T lymphocyte surface were significantly higher in INR group, which implies that the probability of T cell apoptosis was greatly increased (p<0.05). ELISA results showed that the overall production of T cell-related cytokines were lower in INR group, but the amount of cytokine secreted from each T cell was higher in INR group than that in IR group due to the significant decrease of T cell in this group. Furthermore, the proportion and the killing efficiency of CD56dim subgroup of NK cells were significantly higher in INR group. Conclusion: Multiple factors are involved in the immune reconstitution in AIDS patients, including myelosuppression, T cell destruction enhanced through PD-1 pathway, over-activation of T lymphocyte, and higher killing efficiency of CD56dim subgroup of NK cells.
The Use Of The Hungarian Test Your Memory (Tym-hun), Mmse And Adas-cog Tests For Patients With Mild Cognitive Impairment (Mci) In A Hungarian Population: A Cross-sectional Study
Authors: Garbóczy Szabolcs , Magócs Éva , Szőllősi Gergő , Harsányi Szilvia , Anikó Égerházi , Kolozsvári László 
Published: March 2020

Abstract
BACKGROUNDAlzheimer's Disease (AD) is a growing disease process with aging. If we could recognize the disease at an early stage and increase the number of years spent in a better condition through preventive and treatment measures, we could reduce the pressure both directly on families and indirectly on society. There is a need for testing methods that are easy to perform even in general practitioner’s office, inexpensive and non-invasive, which could help early recognition of mental decline. We have selected Test Your Memory (TYM), which has proven to be reliable for detecting AD and mild cognitive impairment (MCI) in several countries. Our study was designed to test the usability of the TYM-HUN comparing with the ADAS-Cog (Alzheimer's Disease Assessment Scale-Cognitive Subscale) in MCI recognition in the Hungarian population.METHODSTYM test was translated and validated into Hungarian (TYM-HUN). The TYM-HUN test was used in conjunction with and compared with the Mini-Mental State Examination (MMSE) and the ADAS-Cog. For our study, 50 subjects were selected, 25 MCI patients and 25 healthy controls. Spearman’s rank correlation was used to analyze the correlation between the scores of MMSE and ADAS-Cog with TYM-HUN.RESULTSMCI can be distinguished from AD and normal aging using ADAS-Cog and MMSE is a useful tool to detect dementia. We established a 'cut-off' point of TYM-HUN (44/45points) where optimal sensitivity and specificity values were obtained to screen MCI. The total TYM-HUN scores significantly correlated with the MMSE scores (ρ=0.626; p<0.001) and ADAS-Cog scores (ρ=-0.723; p<0.001).CONCLUSIONSOur results showed that the Hungarian version of TYM (TYM-HUN) is an easy, fast, self-administered questionnaire with the right low threshold regarding MCI and can be used for the early diagnosis of cognitive impairment.