bluuebunny/crossref_metadata_2025_split huggingface dataset:
Contribution of medico-administrative data to the development of a comorbidity score to predict mortality in End-Stage Renal Disease patients
Authors: Pladys Adélaïde , Vigneau Cécile , Raffray Maxime , Sautenet Bénédicte , Gentile Stéphanie , Couchoud Cécile , Bayat Sahar 
Published: May 2020

AbstractComorbidity scores to predict mortality are very useful to facilitate decision-making for personalized patient management. This study aim was to assess the contribution of medico-administrative data in addition to French Renal Epidemiology and Information Network (REIN) data to the development of a risk score to predict the 1-year all-cause mortality in patients with End Stage Renal Disease (ESRD), and to compare it with previous scores. Data from a derivation sample (n = 6336 patients who started dialysis in 2015 in France) obtained by linking the REIN and the French National Health Insurance Information System databases were analyzed with multivariate Cox models to select risk factors to establish the score. A randomly chosen validation sample (n = 2716 patients who started dialysis in 2015) was used to validate the score and to compare it with the comorbidity indexes developed by Wright and Charlson. The ability to predict one-year mortality of the score constructed using REIN data linked to the medico-administrative database was not higher than that of the score constructed using only REIN data (i.e.,Rennes score). The Rennes score included five comorbidities, albumin, and age. This score (AUC = 0.794, 95%CI: 0.768–0.821) outperformed both the Wright (AUC = 0.631, 95%CI: 0.621–0.639; p < 0.001) and Charlson (AUC = 0.703, 95%CI: 0.689–0.716; p < 0.001) indexes. Data from the REIN registry alone, collected at dialysis start, are sufficient to develop a risk score that can predict the one-year mortality in patients with ESRD. This simple score might help identifying high risk patients and proposing the most adapted care.
Extracellular vesicles from genetically unstable, oncogene-driven cancer cells trigger micronuclei formation in endothelial cells
Authors: Chennakrishnaiah Shilpa , Tsering Thupten , Gregory Caroline , Tawil Nadim , Spinelli Cristiana , Montermini Laura , Karatzas Nicolaos , Aprikian Saro , Choi Dongsic , Klewes Ludger , Mai Sabine , Rak Janusz 
Published: May 2020

AbstractOncogenic transformation impacts cancer cell interactions with their stroma, including through formation of abnormal blood vessels. This influence is often attributed to angiogenic growth factors, either soluble, or associated with tumor cell-derived extracellular vesicles (EVs). Here we examine some of the cancer-specific components of EV-mediated tumor-vascular interactions, including the impact of genetic driver mutations and genetic instability. Cancer cells expressing mutant HRAS oncogene exhibit aberrations of chromatin architecture, aneuploidy, cytoplasmic chromatin deposition and formation of micronuclei with a non-random chromosome content. EVs released from such HRAS-driven cells carry genomic DNA, including oncogenic sequences, and transfer this material to endothelial cells while inducing abnormal formation of micronuclei, along with cell migration and proliferation. Micronuclei were also triggered following treatment with EVs derived from glioma cells (and stem cells) expressing EGFRvIII oncogene, and in both endothelial cells and astrocytes. EVs from HRAS and EGFRvIII-driven cancer cells carry 19 common proteins while EVs from indolent control cells exhibit more divergent proteomes. Immortalized endothelial cell lines with disrupted TP53 pathway were refractory to EV-mediated micronuclei induction. We suggest that oncogenic transformation and intercellular trafficking of cancer-derived EVs may contribute to pathological vascular responses in cancer due to intercellular transmission of genomic instability.
Occupational noise exposure and its association with incident hyperglycaemia: a retrospective cohort study
Authors: Chang Ta-Yuan , Yu Tzu-Yi , Liu Chiu-Shong , Young Li-Hao , Bao Bo-Ying 
Published: May 2020

AbstractNoise pollution is reported to be associated with diabetes, but few studies have elucidated the associations between noise frequency characteristics. We aimed to evaluate the relationships between different noise frequency components and incident hyperglycaemia. An industry-based cohort of 905 volunteers was enrolled and followed up to 2012. Octave-band frequencies of workstation noise and individual noise levels were measured in 2012 to classify subjects’ exposures retrospectively. We applied Cox regression models to estimate the relative risk (RR) of hyperglycaemia. An increased RR for hyperglycaemia of 1.80 (95% confidence interval [CI]: 1.04, 3.10) was found among subjects exposed to ≥ 85 A-weighted decibels (dBA) compared with those exposed to < 70 dBA. The high-exposure groups at frequencies of 31.5, 63, 125, 250, 500, 1000, and 2000 Hz had a significantly higher risk of hyperglycaemia (all p values < 0.050) than the low-exposure groups. A 5-dB increase in noise frequencies at 31.5, 63, 125, 250, 500 Hz, and 1000 Hz was associated with an elevated risk of hyperglycaemia (all p values < 0.050), with the highest value of 1.27 (95% CI: 1.10, 1.47) at 31.5 Hz (p = 0.001). Occupational noise exposure may be associated with an increased incidence of hyperglycaemia, with the highest risk observed at 31.5 Hz.
The strategic combination of trastuzumab emtansine with oncolytic rhabdoviruses leads to therapeutic synergy
Authors: Arulanandam Rozanne , Taha Zaid , Garcia Vanessa , Selman Mohammed , Chen Andrew , Varette Oliver , Jirovec Anna , Sutherland Keara , Macdonald Elizabeth , Tzelepis Fanny , Birdi Harsimrat , Alluqmani Nouf , Landry Anne , Bergeron Anabel , Vanderhyden Barbara , Diallo Jean-Simon 
Published: May 2020

AbstractWe have demonstrated that microtubule destabilizing agents (MDAs) can sensitize tumors to oncolytic vesicular stomatitis virus (VSVΔ51) in various preclinical models of cancer. The clinically approved T-DM1 (Kadcyla®) is an antibody-drug conjugate consisting of HER2-targeting trastuzumab linked to the potent MDA and maytansine derivative DM1. We reveal that combining T-DM1 with VSVΔ51 leads to increased viral spread and tumor killing in trastuzumab-binding, VSVΔ51-resistant cancer cells. In vivo, co-treatment of VSVΔ51 and T-DM1 increased overall survival in HER2-overexpressing, but trastuzumab-refractory, JIMT1 human breast cancer xenografts compared to monotherapies. Furthermore, viral spread in cultured HER2+ human ovarian cancer patient-derived ascites samples was enhanced by the combination of VSVΔ51 and T-DM1. Our data using the clinically approved Kadcyla® in combination with VSVΔ51 demonstrates proof of concept that targeted delivery of a viral-sensitizing molecule using an antibody-drug conjugate can enhance oncolytic virus activity and provides rationale for translation of this approach.
Ferroelectric resistance switching in Pt/Fe/BiFeO3/SrRuO3/SrTiO3 heterostructures
Authors: Zheng Wanchao , Wang Yuchen , Jin Chao , Yin Ruihua , Li Dong , Wang Ping , Liu Shasha , Wang Xinyue , Zheng Dongxing , Bai Haili 
Published: May 2020

The resistive switching behavior in the Pt/Fe/BiFeO3/SrRuO3 heterostructures was observed. It results from the ferroelectric polarization modulated the depletion layer width around the BiFeO3/SrRuO3 interface.
The long non-coding RNA LINC00473 contributes to cell proliferation via JAK-STAT3 signaling pathway by regulating miR-195-5p/SEPT2 axis in prostate cancer
Authors: Xing Zengshu , Li Sailian , Liu Zhenxiang , Zhang Chong , Meng Meijiang , Bai Zhiming 
Published: May 2020

Abstract
Prostate cancer is a kind of male malignant tumor, which has brought tremendous health threat to men. Prostate cancer is difficult to be cured because of high incidence and metastasis rate. Thereby, it is of great urgency to elucidate the underlying molecular mechanism of prostate cancer for the treatment of this cancer. LINC00473 dysregulation has been observed in many cancers. However, the role of LINC00473 was unknown in prostate cancer. In the present study, we discovered that prostate cancer cells presented high expression of LINC00473, and LINC00473 inhibition limited cell proliferation and the expression of proteins in JAK-STAT3 signaling pathway. Additionally, LINC00473 acted as an up-stream factor for miR-195-5p to negatively modulate miR-195-5p expression. Moreover, SEPT2 interacted with miR-195-5p in prostate cancer and SEPT2 expression was positively modulated by LINC00473 and negatively regulated by miR-195-5p. Last, the inhibitory effect of LINC00473 knockdown on cell proliferation and expression of proteins of JAK-STAT3 signaling pathway was restored by SEPT2 overexpression. All in all, LINC00473 contributed to cell proliferation via JAK-STAT3 signaling pathway by regulating miR-195-5p/SEPT2 axis in prostate cancer, which provided a novel therapeutic tactic for prostate cancer patients.
Development of a novel prognostic signature for predicting the overall survival of bladder cancer patients
Authors: Tang Huamei , Kan Lijuan , Ou Tong , Chen Dayang , Dou Xiaowen , Wu Wei , Ji Xiang , Wang Mengmeng , Zong Zengyan , Mo Hongmei , Zhang Xiuming , Xiong Dan 
Published: May 2020

Abstract
Background: Bladder cancer is one of the most common malignancies. So far, no effective biomarker for bladder cancer prognosis has been identified. Aberrant DNA methylation is frequently observed in the bladder cancer and holds considerable promise as a biomarker for predicting the overall survival (OS) of patients. Materials and methods: We downloaded the DNA methylation and transcriptome data for bladder cancer from The Cancer Genome Atlas (TCGA), a public database, screened hypo-methylated and up-regulated genes, similarly, hyper-methylation with low expression genes, then retrieved the relevant methylation sites. Cox regression analysis was used to identify a nine-methylation site signature of a training group. Predictive ability was validated in a test group by receiver operating characteristic (ROC) analysis. Results: We identified nine bladder cancer-specific methylation sites as potential prognostic biomarkers and established a risk score system based on the methylation site signature to evaluate the OS. The performance of the signature was accurate, with area under curve was 0.73 in the training group and 0.71 in the test group. Taking clinical features into consideration, we constructed a nomogram consisting of the nine-methylation site signature and patients’ clinical variables, and found that the signature was an independent risk factor. Conclusions: Overall, the significant nine methylation sites could be novel prediction biomarkers, which could aid in treatment and also predict the overall survival likelihoods of bladder cancer patients.
Comparison of ultrasound-guided supraclavicular and costoclavicular brachial plexus block using a modified double-injection technique: a randomized non-inferiority trial
Authors: Luo Quehua , Yao Weifeng , Chai Yunfei , Chang Lu , Yao Hui , Liang Jiani , Hao Ning , Guo Song , Shu HaiHua 
Published: May 2020

AbstractUltrasound-guided costoclavicular block (CC-approach) is a recently described brachial plexus block (BPB) and an alternative approach to the supraclavicular approach (SC-approach). The relevant sonoanatomy is analogous in terms of the brachial plexus and its adjacent artery for both approaches. In the present study, we hypothesized that the two approaches will result in similar block dynamics when used the modified double-injection (MDI) technique. One hundred and twelve patients were randomly allocated to receive either a SC- or CC-approach with MDI technique. In the CC group, half the volume was injected adjacent to the medial cord of the brachial plexus, the procedure was guided by ultrasound and verified by nerve stimulator, subsequently the second half was injected close to the lateral cord. In the SC group, the MDI technique was carried out as described in our previous study. Sensory and motor blockade of all four terminal nerves were assessed with a 3-point scale. The primary outcome was the proportion of complete sensory blockade at 15 min with a predefined non-inferiority margin of −13%. The proportion of subjects at 15 min was comparable between the SC group and the CC group (91 vs 87%, absolute difference: −3%). No significant differences were found for complete motor blockade and onset times of the individual nerves within 30 min, and block-related serious adverse events (all P&gt;0.05). We conclude that the MDI technique applied to a costoclavicular and supraclavicular block resulted in similar block dynamics. In addition, it may provide a promising alternative technique when considering the use of multipoint injection.
ATP reduces mitochondrial MECR protein in liver of diet-induced obese mice in mechanism of insulin resistance
Authors: Qian Shengnan , Ma Li , Peng Shiqiao , Xu Yanhong , Wu Kaiyue , Shen Shuang , Zhang Xiaoying , Sun Yongning , Ye Jianping 
Published: May 2020

Abstract
Mitochondrial 2-enoyl-acyl-carrier protein reductase (MECR) is an enzyme in the mitochondrial fatty acid synthase (mtFAS) pathway. MECR activity remains unknown in the mechanism of insulin resistance in the pathogenesis of type 2 diabetes. In the present study, MECR activity was investigated in diet-induced obese (DIO) mice. Mecr mRNA was induced by insulin in cell culture, and was elevated in the liver of DIO mice in the presence hyperinsulinemia. However, MECR protein was decreased in the liver of DIO mice, and the reduction was blocked by treatment of the DIO mice with berberine (BBR). The mechanism of MECR protein regulation was investigated with a focus on ATP. The protein was decreased in the cell lysate and DIO liver by an increase in ATP levels. The ATP protein reduction was blocked in the liver of BBR-treated mice by suppression of ATP elevation. The MECR protein reduction was associated with insulin resistance and the protein restoration was associated with improvement of insulin sensitivity by BBR in the DIO mice. The data suggest that MECR protein is regulated in hepatocytes by ATP in association with insulin resistance. The study provides evidence for a relationship between MECR protein and insulin resistance.
Alternative splicing of ACE2 possibly generates variants that may limit the entry of SARS-CoV-2: a potential therapeutic approach using SSOs
Authors: Rehman Sayeed ur , Tabish Mohammad 
Published: May 2020

Abstract
Angiotensin-converting enzyme 2 (ACE2) plays an essential role in maintaining the balance of the renin–angiotensin system and also serves as a receptor for the SARS-CoV-2, SARS-CoV, and HCoV-NL63. Following the recent outbreak of SARS-CoV-2 infection, there has been an urgent need to develop therapeutic interventions. ACE2 is a potential target for many treatment approaches for the SARS-CoV-2. With the help of bioinformatics, we have predicted several novel exons of the human ACE2 gene. The inclusion of novel exons located in the 5′UTR/intronic region in the mature transcript may remove the critical ACE2 residues responsible for the interaction with the receptor-binding domain (RBD) of SARS-CoV-2, thus preventing their binding and entry into the cell. Additionally, inclusion of a novel predicted exons located in the 3′UTR by alternative splicing may remove the C-terminal transmembrane domain of ACE2 and generate soluble ACE2 isoforms. Splice-switching antisense oligonucleotides (SSOs) have been employed effectively as a therapeutic strategy in several disease conditions. Alternative splicing of the ACE2 gene could similarly be modulated using SSOs to exclude critical domains required for the entry of SARS-CoV-2. Strategies can also be designed to deliver these SSOs directly to the lungs in order to minimize the damage caused by SARS-CoV-2 pathogenesis.
Raman distributed temperature sensing for end winding of high‐power generator
Authors: Pelegrin Jessé de , Bazzo João Paulo , Dreyer Uilian José , Martelli Cicero , Pipa Daniel Rodrigues , Silva Erlon Vagner da , Cardozo da Silva Jean Carlos 
Published: May 2020

The temperature on the busbars of electric generators in the end‐winding region needs to be monitored continuously. High temperature is associated with curvatures, seams, and poor ventilation. However, this solution has limitations, such as the number of measurement points. This article presents a reconstruction algorithm to estimate high‐resolution signals, from readings of a distributed temperature system for monitoring high‐power generator bars. The purpose of this work is to measure the temperature in the end‐winding region with optical sensors based on Raman distributed temperature sensing (RDTS). The ability of RDTS equipment to perform a measurement is limited by its spatial resolution of 1 m. The proposed algorithm reduces spatial resolution through signal processing without changing the equipment hardware. Through the experimental results, it was possible to perform accurate temperature measurements for lengths up to 15 cm using the sensor element. With this method, the final winding of a generator bar of a 355 MW hydroelectric plant was instrumented in the laboratory. The results show that it is possible to monitor the temperature over this region, which has a length of 45 cm. Therefore, the proposed method has the potential for the use of RDTS systems for temperature acquisition of small, medium, and large electrical machines.
Effects of process parameters on Cu‐nanoparticle synthesis in tetraethylene glycol through microwave irradiation
Authors: Lim Choi Woo , Lee Jong‐Hyun 
Published: May 2020

Copper nanoparticles were successfully synthesised through microwave irradiation of a copper‐oxide particle precursor dispersed in tetraethylene glycol. Initial results obtained after 4–6 min of irradiation, without the use of a surfactant, indicated nanoparticle aggregation and particle‐size bimodality by incomplete reduction. However, the said aggregation and bimodality were observed to be significantly suppressed with accelerated reduction upon addition of an alcohol‐type surfactant. Consequently, Cu nanoparticles measuring 74 and 79 nm in size were successfully synthesised by performing short irradiations lasting 3 and 4 min, respectively.
Green sol–gel synthesis of CoMnCrO4 spinel nanoparticles and their photocatalytic application
Authors: Moradnia Farzaneh , Taghavi Fardood Saeid , Ramazani Ali , Osali Samira , Abdolmaleki Ilnaz 
Published: May 2020

CoMnCrO4 spinel nanoparticles were prepared using the green and efficient sol–gel method. The synthesised nanoparticles were fully characterised by Fourier‐transform infrared spectroscopy, X‐ray powder diffraction with Rietveld refinement, transmission electron microscopy, scanning electron microscopy, and diffuse reflectance spectroscopy analysis. The characterisation of the sample showed that the CoMnCrO4 NPs have a spinel cubic structure with a crystallite size of 14 nm, narrow bandgap (1.75 eV), and uniform spherical morphology with particle size about 25–30 nm. The CoMnCrO4 NPs demonstrated high photocatalysis capability towards the Congo red dye as a pollutant with a high and fast efficiency degradation of 90% after 30 min under the visible light irradiation. Also, the impacts of different parameters consisting of nanoparticle amount, initial dye concentration, irradiation time, light and dark on dye degradation efficiency were assessed.
New multi-part collisional model of the main belt: the contribution to near-Earth asteroids
Authors: Zain P. S. , de Elía G. C. , Di Sisto R. P. 
Published: May 2020

Aims. We developed a six-part collisional evolution model of the main asteroid belt (MB) and used it to study the contribution of the different regions of the MB to the near-Earth asteroids (NEAs).
Methods. We built a statistical code called ACDC that simulates the collisional evolution of the MB split into six regions (namely Inner, Middle, Pristine, Outer, Cybele and High-Inclination belts) according to the positions of the major resonances present there (ν6, 3:1J, 5:2J, 7:3J and 2:1J). We consider the Yarkovsky effect and the mentioned resonances as the main mechanism that removes asteroids from the different regions of the MB and delivers them to the NEA region. We calculated the evolution of the NEAs coming from the different source regions by considering the bodies delivered by the resonances and mean dynamical timescales in the NEA population.
Results. Our model is in agreement with the major observational constraints associated with the MB, such as the size distributions of the different regions of the MB and the number of large asteroid families. It is also able to reproduce the observed NEAs with H < 16 and agrees with recent estimations for H < 20, but deviates for smaller sizes. We find that most sources make a significant contribution to the NEAs; however the Inner and Middle belts stand out as the most important source of NEAs followed by the Outer belt. The contributions of the Pristine and Cybele regions are minor. The High-Inclination belt is the source of only a fraction of the actual observed NEAs with high inclination, as there are dynamical processes in that region that enable asteroids to increase and decrease their inclinations.
Perfectly parallel cosmological simulations using spatial comoving Lagrangian acceleration
Authors: Leclercq F. , Faure B. , Lavaux G. , Wandelt B. D. , Jaffe A. H. , Heavens A. F. , Percival W. J. 
Published: May 2020

Context.Existing cosmological simulation methods lack a high degree of parallelism due to the long-range nature of the gravitational force, which limits the size of simulations that can be run at high resolution.Aims.To solve this problem, we propose a new, perfectly parallel approach to simulate cosmic structure formation, which is based on the spatial COmoving Lagrangian Acceleration (sCOLA) framework.Methods.Building upon a hybrid analytical and numerical description of particles’ trajectories, our algorithm allows for an efficient tiling of a cosmological volume, where the dynamics within each tile is computed independently. As a consequence, the degree of parallelism is equal to the number of tiles. We optimised the accuracy of sCOLA through the use of a buffer region around tiles and of appropriate Dirichlet boundary conditions around sCOLA boxes.Results.As a result, we show that cosmological simulations at the degree of accuracy required for the analysis of the next generation of surveys can be run in drastically reduced wall-clock times and with very low memory requirements.Conclusions.The perfect scalability of our algorithm unlocks profoundly new possibilities for computing larger cosmological simulations at high resolution, taking advantage of a variety of hardware architectures.
Stress and Coping in Caregivers of Children with RASopathies: Assessment of the Impact of Caregiver Conferences
Authors: Ganetsos Athena , Farrelly Ellyn , Magoulas Pilar , Stevenson David A. 
Published: May 2020

AbstractThe study aimed to assess baseline stress and coping mechanisms among caregivers of children with RASopathies (i.e., cardiofaciocutaneous and Costello's syndrome) and the impact of attending biennial caregiver conferences. Caregivers completed the Perceived Stress Scale, Coping Health Inventory for Parents, and demographic surveys prior to family conferences, and 1- and 6-month postconferences. Baseline stress was increased and associated with child age, parental age, and number of conferences attended. After 1 month, caregiver stress was lowered among men and caregivers attending ≥2 support conferences.