bluuebunny/crossref_metadata_2025_split huggingface dataset:
86 Evaluation of ensiled triticale varieties (‘Taza’ and ‘Bunker’ Triticosecale) and barley (Hordeum vulgare) on performance of backgrounding beef steers
Authors: Ross Brittany E , McKinnon John J , Penner Greg B , McAllister Tim A , Larson Kathy A , Nair Jayakrishnan , Lardner Bart 
Published: December 2019

Abstract
A 3-year study compared two triticale (Triticosecale) varieties, Bunker and Taza, with a conventional barley (Hordeum vulgare) fed as silage to backgrounding beef steers on the basis of crop dry matter (DM) yield, nutritive value, steer performance, and total daily feeding costs. Each year, 240 fall weaned beef steers were stratified by BW (308 ± 4.9 kg) and allocated to 1 of 3 replicated (n = 4) dietary treatments containing Bunker triticale, Taza triticale, or barley silage. Steers were fed a total mixed ration (TMR) consisting of silage (55–68%), barley grain (20–34%), supplement (5%), and (0.4–6%) canola meal (DM basis). Diets were formulated to be isonitrogenous and isocaloric to meet or exceed NRC (2001) requirements for TDN and CP. Crop DM yield of Taza (6750 kg DM ha-1) and Bunker triticale (6592 kg DM ha-1) was similar (P &gt; 0.05), and both were greater (P = 0.01) than barley (6008 kg DM ha-1). Over 2 years, steer ADG, DMI, G:F, and the calculated NEm, and NEg did not differ (P &gt; 0.05) among treatments. Cost per head per day was lowest for Taza fed steers at $1.26 hd-1 d-1, followed by Bunker silage at $1.33 hd-1 d-1, and Barley silage at $1.34 hd-1 d-1. Total cost of gain for Taza, Bunker, and barley fed steers was $0.84, $0.91, and $0.93, per kg of gain, respectively. Study results suggest that Taza and Bunker triticale silage can be used as an alternative to barley silage when fed to backgrounding beef steers.
96 Development of genomics-based precision management model for Hanwoo steers; response to the divergent selection for marbling tested under different dietary energy levels
Authors: Gajaweera Chandima Jayanath , Chung Ki Yong , Lee Seung Hwan 
Published: December 2019

Abstract
Focusing high marble deposition, Hanwoo feedlot system uses high-energy diet over the prolonged fattening period. However, due to the individual genetic variation, around 40% of them are graded into inferior quality grades (QG), despite they utilized the same resources. Therefore, focusing to develop a genomic-based precision management model, this study was to evaluate the response to the divergent selection on genetic merit for marbling score (MS), under different dietary TDN (total digestible nutrient) levels. Total of 112 calves were genotyped and initially grouped for estimated breeding value (high and low) for marbling score (MS-EBV). Subsequently, managed under two levels of feed TDN%, over the growing period, early, middle, and final fattening periods following 2X2 factorial arrangement (figure 1). Carcasses were evaluated for MS, Backfat thickness (BFT) and Korean beef quality grading standard. As the direct response to the selection was significant, the results confirmed the importance of initial genetic grouping of Hanwoo steers for MS-EBV. However, dietary TDN level did not show an effect (P &gt; 0.05) on the MS. Further, no genetic-by-nutrition interaction for MS (P &gt; 0.05) was also observed. The present results showed no correlation response on BFT (P &gt; 0.05), indicating that the selection based on MS-EBV can be used to enhance the MS without undesirable effect on BFT. Ultimate turnover of the Hanwoo feedlot operation is primarily determined by the QGs. The present model shows that the initial grouping for MS-EBV increased the proportion of carcasses graded for higher QGs (QG1++ and QG1+) by approximately 20%. Moreover, there appears to be a potential to increase the proportion of QG 1++ animals among the high-genetic group by further increasing the dietary energy content. Overall, this precision management strategy suggests the importance of adopting an MS based initial genetic grouping system for Hanwoo steers with a subsequent divergent management based on dietary energy level.
89 Evaluating the effect of Ca-gluconate and Ca-butyrate on SCFA absorption and permeability of the gastrointestinal tract
Authors: Watanabe Daniel H , Doelman John , Steele Michael A , Guan Leluo , Penner Greg B 
Published: December 2019

Abstract
The objective was to evaluate short-chain fatty acid (SCFA) absorption and permeability of the gastrointestinal tract (GIT) of heifers infused either with Ca-gluconate or Ca-butyrate. Thirty-two ruminally cannulated beef heifers were fed a common diet (forage-to-concentrate ratio of 50:50) for 28 d and once daily infused with water (ruminal infusion; control), Ca-gluconate embedded in a fat matrix (ruminal infusion; 0.192% BW), unprotected Ca-gluconate (abomasal infusion; 0.077% of BW), and unprotected Ca-butyrate (abomasal infusion; 0.029% BW). Treatments were designed to provide the same amount of butyrate to the small intestine. DMI was restricted to 95% of voluntary DMI on d 8 and was recorded until heifers were slaughtered on d 28. Rumen, jejunum, and colon tissues were collected to determine the rate and pathway of SCFA transport, and for measurement of permeability.14C-acetate and 3H-butyrate absorption across the ruminal and colonic epithelium was measured with no inhibition and under maximal inhibition. Permeability was assessed for the rumen, jejunum and colon using mucosal-to-serosal flux of 14C-mannitol. Initial and final BW were not different (P &gt; 0.60) averaging 388 ± 5.5 kg and 409 ± 6.6 kg, respectively. DMI was not affected by treatment averaging 7.7 kg/d (P = 0.77). Treatment did not affect the flux of acetate or butyrate across the ruminal or colonic epithelium (P &gt; 0.33), but flux rates of SCFA were numerically greater across the ruminal epithelium. Moreover, approximately 50% of the ruminal acetate was transported via bicarbonate-dependent mechanisms. Mannitol flux was not affected by treatment (P &gt; 0.29) and was numerically lower in the rumen and colon than in the jejunum, supporting previous research evaluating permeability across the GIT. According to this experiment, provision of Ca-butyrate or Ca-gluconate in an attempt to increase intestinal butyrate supply does not impact SCFA absorption or permeability of the rumen, jejunum, or colon.
104 Leadership styles of state extension specialists
Authors: Lawyer Amy 
Published: December 2019

Abstract
The focus of this study was the leadership styles of extension specialists. Many studies have been conducted on leadership characteristics of extension agents and administrators, however the current knowledge base concerning leadership behaviors of extension specialists is lacking. Traditionally, specialists were strictly used as a resource for subject matter information, however, changes to cooperative extension have seen specialists move into a position that involves leading agent groups and conducting programing that directly serves the clientele. Many specialists find themselves with newly acquired expectations, yet lack training or educational background to ensure these skills. Using a mixed methodological approach, this sequential explanatory study was conducted using transformational leadership theory, with the purpose of examining current leadership characteristics among extension specialists. The specialists were sent a survey which contained questions relating to educational background, make-up and tenure of their position, and included the Multifactor Leadership Questionnaire (MLQ) to analyze self-perceived transformational leadership characteristics. This survey was followed by a voluntary individual interview. The purpose of the interview was to gain a broader understanding of the group’s leadership perspectives. Although no significant connections could be made concerning demographic information and MLQ leadership scores, the group as a whole registered below average for displaying transformational leadership characteristics, ranking in the 40th percentile for composite MLQ scores compared to the general population. The interview data showed that the specialists agreed with the concepts of transformational leadership, however MLQ scores and anecdotal evidence show that practical application of transformational leadership was lacking. Most participants indicated they did not initially feel prepared for their job, and many indicated that interpersonal relationship skills were used more often than their degree specialization. The findings from this study may help to encourage leadership training focused towards extension specialists, and to emphasize the need for leadership skills within this position.
258 Intake, digestibility and rumen fermentation in sheep offered alfalfa silage alone or alfalfa and tall fescue-mixtures harvested after a killing frost
Authors: Niyigena Valens , Coffey K P , Coblentz W K , Philipp D , Althaber C , Rhein R T , Pruden M C 
Published: December 2019

Abstract
The objective of this study was to determine the effect of harvesting alfalfa and fescue after frost and ensiling mixtures of these forages on silage fermentation characteristics, intake, digestibility and ruminal fermentation by ewes. Forages were harvested in October of 2018, wilted, and blended as either alfalfa alone, 67% alfalfa +33% fescue, 33% alfalfa + 67% fescue, or 100% fescue. Twenty bins were lined with 2 plastic bags, packed (n = 5/trt), stored for 3 months, and then randomly assigned to ewes (n = 20; mean BW = 34.7 ± 6.65 kg) for ad libitum consumption, using 5 animals per treatment. Ewes were given 17 d of adaptation followed by 5 d of total feces and urine collection. Data were analyzed using PROC-MIXED of SAS and orthogonal polynomial trends were used to identify the effects of different proportions of fescue-to-alfalfa silage. Silage total acids, lactate, and acetate increased linearly (P &lt; 0.01) while silage moisture and ammonia decreased linearly (P &lt; 0.01) with increasing proportions of fescue in the diet. The proportions of lactate to total acids (mole/100 moles) increased linearly (P &lt; 0.01) and quadratically (P &lt; 0.05) by adding more fescue to alfalfa. Intake (g/kg BW), digestibility (%), and intake of digestible DM and OM (g/kg BW) decreased linearly (P &lt; 0.05) as fescue proportion was increased in the silages. Ruminal acetate and butyrate (%) increased linearly (P &lt; 0.01) with increasing fescue concentrations in the diet. Ensiling alfalfa with fescue may improve forage fermentation characteristics, but may reduce intake and digestibility in sheep compared to feeding alfalfa silage alone. This study was supported in part by USDA-ARS specific cooperative agreement 58-3655-4-052.
257 Effects of feeding different levels of quebracho (Schinopsis balansae) extract in a high-roughage total mixed ration on seasonal manure gas emissions
Authors: Norris Aaron B , Tedeschi Luis O , Foster Jamie L , Muir James P , Pinchak Bill E 
Published: December 2019

Abstract
Our objective was to investigate how quebracho tannin (QT; Schinopsis balansae) fed at differing rates within a roughage-based diet affected manure gas emissions of growing steers. The utilization of ruminant species is required for efficient production of human-edible protein to meet global nutrient demands. However, gaseous byproducts from ruminant production systems, such as carbon dioxide (CO2), methane (CH4), and nitrous oxide (N2O) are deleterious to the environment. It is estimated that CO2, CH4, and N2O from managed waste and grazed lands account for roughly 54% of emissions from the livestock sector within the United States, with approximately 80% being associated with beef and dairy cattle. Supplementation of condensed tannins is a plausible method for improving environmental efficiency through the mitigation of enteric CH4 and shifting route of N excretion. During two experimental periods, 12 crossbred steers (236 ± 16 kg) were fed a roughage-based diet at maintenance level with the addition of QT at 0, 1.5, 3, and 4.5% of DM serving as dietary treatments. Following adaptation, feces were collected and gas fluxes measured using vented static chamber technique with manure incubated on the soil surface at two locations, College Station and Stephenville, during two periods corresponding to winter and spring. The daily flux of CO2 was influenced by soil moisture and temperature (r = 0.34; P &lt; 0.01), whereas CH4 and N2O were associated with soil moisture. Cumulative CO2 and gross CO2 equivalent (CO2e) exhibited a dietary treatment effect at College Station (P ≤ 0.01), with a linear reduction with increased dietary QT. At both locations, we observed significance or tendencies for period effects for CO2, CH4, N2O, and CO2e (P ≤ 0.07) with period 2 having higher gas production. Within certain environments, QT supplementation could potentially reduce manure gas emissions, but additional investigation into animal variation is required.
349 Empty body composition and retained energy of Jersey steers using an aggressive implant strategy
Authors: Kirkpatrick Tylo J , Wesley Kaitlyn , Pillmore Sierra L , Cooper Kimberly , Tennant Travis , Lawrence Ty 
Published: December 2019

Abstract
This experiment was designed to quantify the empty body composition of Jersey steers administered an aggressive implant strategy. Jersey steers {n = 30; initial body weight (BW) 183 ± 43 kg} were randomly assigned to one of two implant strategies: negative control (CON), or implanted with Revalor 200 (200 mg trenbalone acetate / 20 mg estradiol 17-β; (REV) every 70 d (d 0, d 70, d 140, d 210, d 280, d 350) during a 420 d feeding period. Steers were harvested on d 421; 6 CON and 6 REV steers were randomly selected for collection of blood, hide, ground viscera, bone, and ground lean and fat to determine empty body composition. Proximate analysis was completed for each sample to determine total body percentages of moisture, crude protein, fat, and ash. Data were analyzed via independent t-test. Percentage empty body moisture (46.48% CON vs 49.69% REV) and empty body protein (15.32% CON vs 17.58% REV) were greater (P &lt; 0.01) in REV cattle. In contrast empty body fat (33.51% CON vs 26.93% REV) was greater (P &lt; 0.01) for CON cattle. Empty body ash did not differ (P &gt; 0.10; 4.69% CON vs 5.80% REV) between treatments. Negative control steers contained a total empty body protein to total empty body fat ratio of 0.44:1 compared to 0.62:1 for REV steers. These data suggest that an aggressive implant strategy alters composition of gain during the finishing of Jersey steers toward increased protein and decreased fat.
343 Wnt/β-catenin pathway is required for porcine satellite cell proliferation and differentiation to promote skeletal muscle growth
Authors: Zhang Zong-ming , Gao Chun-qi , Yan Hui-chao , Wang Xiu-qi 
Published: December 2019

Abstract
Wnt/β-catenin plays a crucial role in skeletal muscle growth, but its specific mechanism still unclear. In this study, due to the distinct role of lysine in pig industry, we provided it as an entry point to investigate the role of Wnt/β-catenin in governing skeletal muscle growth. Firstly, total 18 weaned piglets were divided into three groups: control group, lysine deficiency group and lysine re-supplementation group (lysine levels added from 0.83% to 1.31% at 14 d). After 28 d experiment, all pigs were slaughtered to measure the change of Wnt/β-catenin in skeletal muscle. Secondly, satellite cell (SC) was isolated and cultured with Wnt activator, such as Wnt3a and WRN (Wnt3a, R-spondin1, Noggin) after lysine deficiency for 48 h to investigate cell proliferation and differentiation ability and the level of Wnt/β-catenin in different conditions. The results showed that compared with the control group, lysine deficiency significantly reduced longissimus dorsi muscle weight and Pax7 positive SC, and inhibited Wnt/β-catenin (P &lt; 0.05). Fortunately, these restrictions were rescued to the control levels by lysine re-supplementation (P &gt; 0.05). Meanwhile, compared with the lysine deficiency group, the MTT and western blotting assay showed cell proliferation ability was significantly increased with re-activated Wnt/β-catenin by re-supplemented lysine, Wnt3a or WRN (P &lt; 0.05), respectively. Moreover, under the condition of cell differentiation, compared with the control group, cell fusion index was significantly decreased in the lysine deficiency group (P &lt; 0.05), whereas it was significantly increased with lysine re-supplementation group, Wnt3a or WRN respective supplementation group in comparison with the lysine deficiency group (P &lt; 0.05). In addition, compared with the lysine deficiency group, the protein levels of myogenic regulatory factors and Wnt/β-catenin pathway were also re-activated by re-supplemented lysine, Wnt3a or WRN (P &lt; 0.05). Collectively, we found Wnt/β-catenin activation is required for porcine SC proliferation and differentiation to promote skeletal muscle growth.
346 Supplementing Ca salts of soybean oil to enhance carcass quality of beef cattle via metabolic imprinting events
Authors: Schubach Kelsey , Cooke Reinaldo F , Brandão Alice , Bohnert David , Marques Rodrigo 
Published: December 2019

Abstract
This experiment evaluated the effects of supplementing Ca salts of soybean oil (CSSO) at 2 mo of age via creep-feeding, upon weaning at 6 months of age, or no CSSO supplementation on growth and carcass development of beef cattle. A total of 64 steers were enrolled in this study over 2 years (32 steers/year), with 4 phases per year: creep-feeding (d 0 to 60), pre-weaning (d 61 to 120), post-weaning (d 121 to 180) and feedlot (181 to slaughter). On d 0 steers were ranked by body weight (BW) and age (114 ± 2 kg; 66 ± 0.5 d) and randomly assigned, in a 2 x 2 factorial, to receive 80 g/steer daily (creep-feeding) or 150 g/steer daily (post-weaning) of CSSO. Non-supplemented steers (CON) were provided with equivalent saturated fat supplement. On d 0, 61, 120, 180, and 330 steers were evaluated for longissimus muscle depth, backfat thickness, and marbling via real time ultrasonography, blood was sampled for determination of plasma fatty acid composition, muscle samples were collected via needle biopsy, and BW was recorded. After the creep-feeding phase, CSSO steers had greater (P &lt; 0.01) plasma concentrations of linoleic, omega-6, and total PUFA compared with CON. Steers that received CSSO during the postweaning phase had greater (P &lt; 0.01) plasma concentrations of linoleic, omega·-6, and total FA compared with CON prior to feedlot entry. No treatment differences were detected for performance or body composition responses (P &gt; 0.25) during any phase. However, mRNA expression of adipogenic/lipogenic genes was greater (P &lt; 0.03) prior to slaughter (d 330) in muscle of steers that had received CSSO during the creep-feeding phase. Results from this experiment suggest that supplementing CSSO to nursing beef steers via creep feeding stimulated metabolic imprinting effects through enhanced adipogenic gene expression, however did not alter phenotypic variables.
69 The ability of an artificial sweetener (Sucram®) to influence microbial community structure in the rumen papillae and content through the production of microbial-based neurochemicals
Authors: Lyte Mark , Koester Lucas , Schmitz-Esser Stephan 
Published: December 2019

Abstract
The study of microorganisms to produce and utilize neurochemical signaling molecules that interact with the host is the emerging field known as microbial endocrinology. Production of these molecules can be regulated by several different environmental factors, including diet. The effect of artificial sweeteners on ruminant gastrointestinal tract microbiota and the ability of individual members to produce neurochemicals that may determine community composition and affect host physiology are unknown. To analyze whether an artificial sweetener (Sucram®, Pancosma, Switzerland) affects rumen content (RC) and rumen papillae (RP) microbiota, six fistulated, lactating Holstein cows were sampled before (baseline) and after exposure to Sucram®. 16S rRNA gene sequencing was conducted to identify Sucram®-induced microbial community changes. Additionally, ex vivo microbial cultures were used to identify neurochemical production in RP bacteria. Exposure to Sucram significantly increased the abundance of Operational Taxonomic Units (OTUs) belonging to Ruminobacter, Prevotella, Sharpea, Ruminococcus and Rikenella on the RP. These organisms have been reported to aid in digestion of feedstuffs and methane reduction, suggesting that Sucram® may induce beneficial shifts in rumen microbial communities. To gain preliminary insight into neurochemical production of RP microorganisms, we tested four different Lactobacillus isolates from RP for neurochemical production. We observed that the neurochemicals DOPAC (3,4-Dihydroxyphenylacetic acid) and L-DOPA (L-3,4-dihydroxyphenylalanine) were produced by three and four isolates, respectively. Ongoing experiments are evaluating the effects of Sucram® on neurochemical production in a larger number of rumen microbes. Overall, we observed significant differences in OTU abundance in response to the addition of Sucram®. Additionally, we confirmed that RP bacteria can produce neurochemicals. Both of these results are key to understanding how Sucram® modifies microbial communities within the rumen and possibly influences host physiology. Research into microbial endocrinology-based neurochemical signaling between rumen microbiota and their animal hosts may lead to advancement of livestock feed efficiency and welfare.
67 Immunometabolism – emerging concepts and potential applications in livestock
Authors: Bradford Barry J 
Published: December 2019

Abstract
Our understanding of the immune system emerged from the study of disease processes and the communication networks used by various cell types to respond to pathogens. As with many aspects of physiology, this initial view was colored by the techniques available at the time. With technical advances beginning in the 1990, research in sepsis and obesity began to identify critical interactions between the immune system and metabolism. Our current understanding of these interactions is informed by two active but largely distinct research communities. Many in the field of immunology are utilizing cellular metabolism tools to understand mitochondrial function and fuel use in response to activation of innate and adaptive immune cells, especially as these relate to cancer. From another vantage point, many metabolic physiologists are now seeking to understand the importance of tissue-resident immune cells and immune signaling molecules in metabolic homeostasis and pathologies. Beyond human health implications of recent findings, a number of immunometabolism insights have informed our understanding of livestock health. In inflammatory events, phagocytic cells are activated, and the dramatic increase in oxidative metabolism is driven primarily by glucose use. Metabolism of healthy animals is also influenced by secretions from immune cells. Studies in mice indicate that appropriate host/microbe interactions (balancing protection and tolerance) are mediated by a network of immune cell types in the gut, which is critical to both absorptive and barrier functions of the gut. Adipose tissue immune cells regulate lipolytic rate, insulin sensitivity, and perhaps whole-body inflammatory tone. Local immune cell impacts on metabolism of other organs, including the liver and pancreas, are also emerging. Immunity and metabolism are tightly interwoven, and the evolving understanding of these links may enable nutritional or pharmacological strategies to enhance resilience to disease and alter nutrient partitioning in livestock.