bluuebunny/crossref_metadata_2025_split huggingface dataset:
From discovery to delivery: public sector development of the rVSV-ZEBOV Ebola vaccine
Authors: Herder Matthew , Graham Janice E , Gold Richard 
Published: December 2019

Abstract
The discovery and development of the Ebola rVSV-ZEBOV vaccine challenge the common assumption that the research and development for innovative therapeutic products and vaccines is best carried out by the private sector. Using internal government documents obtained through an access to information request, we analyze the development of rVSV-ZEBOV by researchers at Canada’s National Microbiology Laboratory beyond its patenting and licensing to a biotech company in the United States in 2010. According to government documentation, the company failed to make any progress toward a phase 1 clinical trial until after the WHO Public Health Emergency of International Concern freed substantial donor and public funds for the vaccine’s further development. The development of rVSV-ZEBOV, from sponsoring early stage research through to carrying out clinical trials during the epidemic, was instead the result of the combined efforts of the Canadian government, its researchers, and other publicly funded institutions. This case study of rVSV-ZEBOV underscores the significant public contribution to the R&D of vaccines even under conditions of precarity, and suggests that an alternative approach to generating knowledge and developing interventions, such as open science, is required in order to fully realize the public sector’s contribution to improved global health.
Quantitative and Qualitative Costs of Autogeny in Phlebotomus papatasi (Diptera: Psychodidae) Sand Flies
Authors: Shymanovich Tatsiana , Hajhashemi Nima , Wasserberg Gideon 
Published: December 2019

Abstract
Most sand flies and mosquitoes require a bloodmeal for egg production, but when blood-sources are scarce, some of them can reproduce without it, so called facultative autogeny. The evolution of autogenous reproduction is thought to involve a trade-off between the benefit of reproducing in the absence of bloodmeal hosts versus the quantitative cost of reduced fecundity and/or or qualitative effect on reduced offspring development and survivorship. We blood-fed (BF) some Phlebotomous papatasi (Scopoli) sand fly females on mice while keeping others (from the same cohort) not BF. We then compared the fecundity of BF and non-blood-fed (NBF) females and also evaluated their egg mass and hatching rate, larval development rate and survivorship, pupa mass and eclosion rates, and progeny fecundity. Among NBF females, only 55% became gravid and produced three times less mature oocytes than BF ones. Autogenous females laid 3.5 and 5.7 times fewer eggs in individual and multi-female bioassays, respectively. Egg mass and hatching rate were not affected by blood-feeding. Individual-larvae bioassays suggested reduced survival during larval stages in the autogenous group. In multi-larvae bioassays, overall and especially pupae survival was significantly reduced in the autogenous group. Development rate was slower and pupal mass was reduced in progeny from autogenous mothers. These effects were particularly apparent at high larval density. Mothers’ blood-feeding history did not affect daughter’s fecundity. Studies on the costs of autogeny provides insights on the evolution of blood feeding. Moreover, it also provides insights regarding potential implications of autogeny to the emergence of vector-borne diseases.
Reduced Protumorigenic Tumor-Associated Macrophages With Statin Use in Premalignant Human Lung Adenocarcinoma
Authors: Al Dujaily Esraa , Baena Juvenal , Das Madhumita , Sereno Marco , Smith Claire , Kamata Tamihiro , Officer Leah , Pritchard Catrin , Le Quesne John 
Published: December 2019

Abstract

Background
Statins have anticancer properties by acting as competitive inhibitors of the mevalonate pathway. They also have anti-inflammatory activity, but their role in suppressing inflammation in a cancer context has not been investigated to date.

Methods
We have analyzed the relationship between statin use and tumor-associated macrophages (TAMs) in a cohort of 262 surgically resected primary human lung adenocarcinomas. TAMs were evaluated by multiplex immunostaining for the CD68 pan-TAM marker and the CD163 protumorigenic TAM marker followed by digital slide scanning and partially automated quantitation. Links between statin use and tumor stage, virulence, and cancer-specific survival were also investigated in a wider cohort of 958 lung adenocarcinoma cases. All statistical tests were two-sided.

Results
We found a statin dose-dependent reduction in protumorigenic TAMs (CD68+CD163+) in both stromal (P = .021) and parenchymal (P = .003) compartments within regions of in situ tumor growth, but this association was lost in invasive regions. No statistically significant relationship between statin use and tumor stage was observed, but there was a statin dose-dependent shift towards lower histological grade as assessed by growth pattern (P = .028). However, statin use was a predictor of slightly worse cancer-specific survival (P = .032), even after accounting for prognostic variables in a multivariable Cox proportional hazards survival model (hazard ratio = 1.38, 95% confidence interval = 1.04 to 1.84).

Conclusions
Statin use is associated with reduced numbers of protumorigenic TAMs within preinvasive lung adenocarcinoma and is related to reduced tumor invasiveness, suggesting a chemo-preventive effect in early tumor development. However, invasive disease is resistant to these effects, and no beneficial relationship between statin use and patient outcome is observed.
Stellar population properties of individual massive early-type galaxies at 1.4 &lt; z &lt; 2
Authors: Lonoce I , Maraston C , Thomas D , Longhetti M , Parikh T , Guarnieri P , Comparat J 
Published: December 2019

ABSTRACT
We analyse publicly available, individual spectra of four massive ($M\gt 10^{11}\, \mathrm{M}_{\odot }$) early-type galaxies with redshifts in the range 1.4 ≤ z ≤ 2 to determine their stellar content, extending our previous work up to z ∼ 2. The wide wavelength range of the VLT/X-Shooter spectroscopic data in the UV–Optical–NIR arms along with the availability of spectro-photometry allows us to explore different techniques to obtain the stellar population properties, namely through age/metallicity-sensitive spectral indices, full spectral fitting, and broad-band photometric fitting. Moreover, together with the widely used optical Lick indices, we consider further indices in the UV rest frame, and demonstrate that UV indices significantly help the accuracy of the resulting population parameters. We find galaxy ages ranging from 0.2 to 4 Gyr, where the oldest galaxy is found at the lowest redshift, with an excellent agreement between ages determined via indices, full spectral fitting, or broad-band colours. These ages are in perfect agreement with ages of local galaxies at the same velocity dispersion when we assume pure passive evolution. Total metallicities derived from indices show some scatter (between less than half-solar to very high values, [Z/H] ∼ 0.6). We speculate on possible mechanisms explaining these values, but given the sample size and low S/N of the spectra no conclusion can be made. Indices in the UV rest frame generally lead to similar conclusions as optical indices. For the oldest galaxy (4 Gyr), we show that its UV indices can only be explained by stellar population models including a UV contribution from old stellar populations, suggesting that old, UV bright populations start to inhabit mature galaxies of a few Gyr of age. This is the highest redshift (z ∼ 1.4) detection of the UV upturn up to date.
Sardinia Radio Telescope observations of Local Group dwarf galaxies – I. The cases of NGC 6822, IC 1613, and WLM
Authors: Tarchi A , Castangia P , Surcis G , Brunthaler A , Henkel C , Pawlowski M , Menten K M , Melis A , Casu S , Murgia M , Trois A , Concu R , Darling J 
Published: December 2019

ABSTRACT
Almost all dwarf galaxies in the Local Group (LG) that are not satellites of the Milky Way or M 31 belong to either one of two highly symmetric planes. It is still a matter of debate whether these planar structures are dynamically stable or whether they only represent a transient alignment. Proper motions, if they could be measured, could help to discriminate between these scenarios. Such motions could be determined with multi-epoch very long baseline interferometry (VLBI) of sources that show emission from water and methanol at frequencies of 22 and 6.7 GHz, respectively. We report searches for such masers. We have mapped three LG galaxies, NGC 6822, IC 1613, and WLM, in the bands covering the water vapour and methanol lines. These systems are members of the two above-mentioned planes of galaxies. We have produced deep radio continuum (RC) maps and spectral line cubes. The former have been used to identify star-forming regions and to derive global galactic star formation rates (SFRs). These SFRs turn out to be lower than those determined at other wavelengths in two of our sources. This indicates that dwarf galaxies may follow predictions on the RC–SFR relation only in individual regions of enhanced RC emission, but not when considering the entire optical body of the sources. No methanol or water maser emission has been confidently detected, down to line luminosity limits of ∼4 × 10−3 and 10 × 10−3 L⊙, respectively. This finding is consistent with the small sizes, low SFRs, and metallicities of these galaxies.
The Young Suns Exoplanet Survey: Detection of a wide-orbit planetary-mass companion to a solar-type Sco-Cen member
Authors: Bohn A J , Kenworthy M A , Ginski C , Manara C F , Pecaut M J , de Boer J , Keller C U , Mamajek E E , Meshkat T , Reggiani M , Todorov K O , Snik F 
Published: December 2019

ABSTRACT
The Young Suns Exoplanet Survey consists of a homogeneous sample of 70 young, solar-mass stars located in the Lower Centaurus-Crux subgroup of the Scorpius-Centaurus association with an average age of 15 ± 3 Myr. We report the detection of a co-moving companion around the K3IV star TYC 8998-760-1 (2MASSJ13251211–6456207) that is located at a distance of 94.6 ± 0.3 pc using SPHERE/IRDIS on the VLT. Spectroscopic observations with VLT/X-SHOOTER constrain the mass of the star to $1.00\pm 0.02\, \mathrm{M}_{\odot }$ and an age of $16.7\pm 1.4\,$ Myr. The companion TYC 8998-760-1 b is detected at a projected separation of 1.71″, which implies a projected physical separation of 162 au. Photometric measurements ranging from Y to M band provide a mass estimate of $14\pm 3\, M_\mathrm{jup}$ by comparison to BT-Settl and AMES-dusty isochrones, corresponding to a mass ratio of q = 0.013 ± 0.003 with respect to the primary. We rule out additional companions to TYC 8998-760-1 that are more massive than $12\, M_\mathrm{jup}$ and farther than 12 au away from the host. Future polarimetric and spectroscopic observations of this system with ground and space based observatories will facilitate testing of formation and evolution scenarios shaping the architecture of the circumstellar environment around this ‘young Sun’.
The Hi-GAL catalogue of dusty filamentary structures in the Galactic plane
Authors: Schisano Eugenio , Molinari S , Elia D , Benedettini M , Olmi L , Pezzuto S , Traficante A , Brescia M , Cavuoti S , di Giorgio A M , Liu S J , Moore T J T , Noriega-Crespo A , Riccio G , Baldeschi A , Becciani U , Peretto N , Merello M , Vitello F , Zavagno A , Beltrán M T , Cambrésy L , Eden D J , Li Causi G , Molinaro M , Palmeirim P , Sciacca E , Testi L , Umana G , Whitworth A P 
Published: December 2019

ABSTRACT
The recent data collected by Herschel have confirmed that interstellar structures with a filamentary shape are ubiquitously present in the Milky Way. Filaments are thought to be formed by several physical mechanisms acting from large Galactic scales down to subparsec fractions of molecular clouds, and they might represent a possible link between star formation and the large-scale structure of the Galaxy. In order to study this potential link, a statistically significant sample of filaments spread throughout the Galaxy is required. In this work, we present the first catalogue of 32 059 candidate filaments automatically identified in the Herschel Infrared Galactic plane Survey (Hi-GAL) of the entire Galactic plane. For these objects, we determined morphological (length la and geometrical shape) and physical (average column density $N_{\rm H_{2}}$ and average temperature T) properties. We identified filaments with a wide range of properties: 2 ≤ la ≤ 100 arcmin, $10^{20} \le N_{\rm H_{2}} \le 10^{23}$ cm−2 and 10 ≤ T ≤ 35 K. We discuss their association with the Hi-GAL compact sources, finding that the most tenuous (and stable) structures do not host any major condensation. We also assign a distance to ∼18 400 filaments, for which we determine mass, physical size, stability conditions and Galactic distribution. When compared with the spiral arms structure, we find no significant difference between the physical properties of on-arm and inter-arm filaments. We compare our sample with previous studies, finding that our Hi-GAL filament catalogue represents a significant extension in terms of Galactic coverage and sensitivity. This catalogue represents a unique and important tool for future studies devoted to understanding the filament life-cycle.
Galaxy interactions in IllustrisTNG-100, I: The power and limitations of visual identification
Authors: Blumenthal Kelly A , Moreno Jorge , Barnes Joshua E , Hernquist Lars , Torrey Paul , Claytor Zachary , Rodriguez-Gomez Vicente , Marinacci Federico , Vogelsberger Mark 
Published: December 2019

ABSTRACT
We present a sample of 446 galaxy pairs constructed using the cosmological simulation IllustrisTNG-100 at z = 0, with M$_{\rm FoF,dm} = 10^{11}\!-\!10^{13.5}$ M⊙. We produce ideal mock SDSS g-band images of all pairs to test the reliability of visual classification schema employed to produce samples of interacting galaxies. We visually classify each image as interacting or not based on the presence of a close neighbour, the presence of stellar debris fields, disturbed discs, and/or tidal features. By inspecting the trajectories of the pairs, we determine that these indicators correctly identify interacting galaxies ∼45 per cent of the time. We subsequently split the sample into the visually identified interacting pairs (VIP; 38 pairs) and those which are interacting but are not visually identified (nonVIP; 47 pairs). We find that VIP have undergone a close passage nearly twice as recently as the non-VIP, and typically have higher stellar masses. Further, the VIP sit in dark matter haloes that are approximately 2.5 times as massive, in environments nearly 2 times as dense, and are almost a factor of 10 more affected by the tidal forces of their surroundings than the nonVIP. These factors conspire to increase the observability of tidal features and disturbed morphologies, making the VIP more likely to be identified. Thus, merger rate calculations which rely on stellar morphologies are likely to be significantly biased toward massive galaxy pairs which have recently undergone a close passage.
Differential expression of duplicated ribosomal protein genes modifies ribosome composition in response to stress
Authors: Ghulam Mustafa Malik , Catala Mathieu , Abou Elela Sherif 
Published: December 2019

AbstractIn Saccharomyces cerevisiae, most ribosomal proteins are synthesized from duplicated genes, increasing the potential for ribosome heterogeneity. However, the contribution of these duplicated genes to ribosome production and the mechanism determining their relative expression remain unclear. Here we demonstrate that in most cases, one of the two gene copies generate the bulk of the active ribosomes under normal growth conditions, while the other copy is favored only under stress. To understand the origin of these differences in paralog expression and their contribution to ribosome heterogeneity we used RNA polymerase II ChIP-Seq, RNA-seq, polyribosome association and peptide-based mass-spectrometry to compare their transcription potential, splicing, mRNA abundance, translation potential, protein abundance and incorporation into ribosomes. In normal conditions a post-transcriptional expression hierarchy of the duplicated ribosomal protein genes is the product of the efficient splicing, high stability and efficient translation of the major paralog mRNA. Exposure of the cell to stress modifies the expression ratio of the paralogs by repressing the expression of the major paralog and thus increasing the number of ribosomes carrying the minor paralog. Together the data indicate that duplicated ribosomal protein genes underlie a modular network permitting the modification of ribosome composition in response to changing growth conditions.
Managing pain and distress in children undergoing brief diagnostic and therapeutic procedures
Authors: Trottier Evelyne D , Doré-Bergeron Marie-Joëlle , Chauvin-Kimoff Laurel , Baerg Krista , Ali Samina 
Published: December 2019

AbstractCommon medical procedures to assess and treat patients can cause significant pain and distress. Clinicians should have a basic approach for minimizing pain and distress in children, particularly for frequently used diagnostic and therapeutic procedures. This statement focuses on infants (excluding care provided in the NICU), children, and youth who are undergoing common, minor but painful medical procedures. Simple, evidence-based strategies for managing pain and distress are reviewed, with guidance for integrating them into clinical practice as an essential part of health care. Health professionals are encouraged to use minimally invasive approaches and, when painful procedures are unavoidable, to combine simple pain and distress-minimizing strategies to improve the patient, parent, and health care provider experience. Health administrators are encouraged to create institutional policies, improve education and access to guidelines, create child- and youth-friendly environments, ensure availability of appropriate staff, equipment and pharmacological agents, and perform quality audits to ensure pain management is optimal.
Iron requirements in the first 2 years of life
Authors: Unger Sharon L , Fenton Tanis R , Jetty Radha , Critch Jeff N , O’connor Deborah L 
Published: December 2019

Abstract
Iron is an essential micronutrient required for hemoglobin synthesis, central nervous system development, and protection from infection. Early childhood is a time of vulnerability as iron deficiency in this period is associated with impaired neurodevelopment. Low socioeconomic status, preterm birth, and suboptimal diet are risk factors for iron deficiency. Due to a lack of iron excretory mechanism, the possibility of iron excess also exists. Appropriate iron intake in the first 2 years of life is critical.
Coping with the extremes: comparative osteology of the tepui-associated toad Oreophrynella and its bearing on the evolution of osteological novelties in the genus
Authors: Kok Philippe J R , van der Velden Milan A J , Means D Bruce , Ratz Sebastian , Josipovic Iván , Boone Matthieu , McDiarmid Roy W 
Published: December 2019

AbstractThe only study of the osteology of the toad genus Oreophrynella dates back to 1971 and was based on a single species. Here, we use high-resolution X-ray microcomputed tomography to analyse the osteology of all nine described Oreophrynella species, which are compared with representatives of other bufonid lineages. Oreophrynella is unique among bufonids in having opposable digits. Osteological synapomorphies confirmed for the genus are as follows: presence of parietal fontanelles and exposed frontoparietal fontanelle, absence of quadratojugal, five presacral vertebrae, distally enlarged terminal phalanges and urostyle greatly expanded into flanges. Ancestral character reconstruction indicates that arboreal habits in some Oreophrynella species are likely to have evolved after the evolution of opposable digits. Opposable digits, in combination with an extension of the interdigital integument and the relative length/orientation of the digits, are likely to be adaptations to facilitate life on rocky tepui summits and an exaptation to arboreality. Cranial simplification in Oreophrynella, in the form of cranial fontanelles and absence of the quadratojugal, is possibly driven by a reduction of developmental costs, increase in flexibility and reduction of body weight. Cranial simplification combined with the shortening of the vertebral column and the shift towards a partly firmisternal girdle might be adaptations to the peculiar tumbling behaviour displayed by Oreophrynella.
Cigarette smoke inhibits the NLRP3 inflammasome and leads to caspase‐1 activation via the TLR4‐TRIF‐caspase‐8 axis in human macrophages
Authors: Buscetta Marco , Di Vincenzo Serena , Miele Monica , Badami Ester , Pace Elisabetta , Cipollina Chiara 
Published: December 2019

AbstractThe NLRP3 inflammasome is formed by the sensor NLRP3, the adaptor ASC, and pro‐caspase‐1. Assembly and activation of the inflammasome trigger caspase‐1‐dependent cleavage of pro‐IL‐1β and pro‐IL‐18 into their secreted forms. Cigarette smoke is a risk factor for chronic inflammatory diseases and is associated with macrophage dysfunction. The impact of cigarette smoke on NLRP3‐dependent responses in macrophages is largely unknown. Herein, we investigated the effects of cigarette smoke extract (CSE) on the NLRP3 inflammasome in human monocyte‐derived macrophages (MDMs) and THP‐1 cells stimulated with lipopolysaccharide (LPS) and LPS plus the NLRP3 inflammasome activator ATP. We found that CSE inhibited the release of IL‐1β and IL‐18 as well as the expression of NLRP3 acting mainly at the transcriptional level. Interestingly, we found that CSE increased the caspase‐1 activity via an NLRP3‐independent and TLR4‐TRIF‐caspase‐8‐dependent pathway. Activation of caspase‐1 by CSE led to a reduction of the basal glycolytic flux and impaired glycolytic burst in response to LPS. Overall, our findings unveil novel pathways leading to immune‐metabolic alterations in human macrophages exposed to cigarette smoke. These mechanisms may contribute to macrophage dysfunction and increased risk of infection in smokers.