bluuebunny/crossref_metadata_2025_split huggingface dataset:
The role of risk‐benefit and privacy analysis to understand different uses of social media by Generations X, Y, and Z in Indonesia
Authors: Nuzulita Nania , Subriadi Apol Pribadi 
Published: December 2019

AbstractThis research aims to analyse how perceived benefits and risks drive social media use despite the concern for privacy violations in Indonesia. The focus of the study is to investigate the pattern of social media use based on generational age groups. An approach based on the combination of Privacy Calculus Theory and Communication Privacy Management is used to explain the patterns of social media use. Privacy awareness and information manipulation help solving the constant social media use despite the concern for privacy violations. The findings show that Generation X uses social media mainly to maintain existing relationships. Generation Y uses social media for business and building self‐image. Generation Z uses social media for more varied purposes, eg, developing and maintaining connections, building self‐image, expressing thoughts and emotions, and seeking entertainment. Further research can use the proposed conceptual model as a quantitative approach to get broader perspectives on the issue and add demographic factors. Future research can also highlight the types of social media to generate more real results.
Cytotoxicity of vanadium oxide nanoparticles and titanium dioxide‐coated vanadium oxide nanoparticles to human lung cells
Authors: Xi Wen‐Song , Tang Huan , Liu Yuan‐Yuan , Liu Chun‐Yuan , Gao Yanfeng , Cao Aoneng , Liu Yuanfang , Chen Zhang , Wang Haifang 
Published: December 2019

AbstractDue to excellent metal–insulator transition property, vanadium dioxide nanoparticles (VO2 NPs)‐based nanomaterials are extensively studied and applied in various fields, and thus draw safety concerns of VO2 NPs exposure through various routes. Herein, the cytotoxicity of VO2 NPs (N‐VO2) and titanium dioxide‐coated VO2 NPs (T‐VO2) to typical human lung cell lines (A549 and BEAS‐2B) was studied by using a series of biological assays. It was found that both VO2 NPs induced a dose‐dependent cytotoxicity, and the two cell lines displayed similar sensitivity to VO2 NPs. Under the same conditions, T‐VO2 NPs showed slightly lower cytotoxicity than N‐VO2 in both cells, indicating the surface coating of titanium dioxide mitigated the toxicity of VO2 NPs. Titanium dioxide coating changed the surface property of VO2 NPs and reduced the vanadium release of particles, and thus helped lowing the toxicity of VO2 NPs. The induced cell viability loss was attributed to apoptosis and proliferation inhibition, which were supported by the assays of apoptosis, mitochondrial membrane damage, caspase‐3 level, and cell cycle arrest. The oxidative stress, i.e., enhanced reactive oxygen species generation and suppressed reduced glutathione , in A549 and BEAS‐2B cells was one of the major mechanisms of the cytotoxicity of VO2 NPs. These findings provide safety guidance for the practical applications of vanadium dioxide‐based materials.
Preclinical safety evaluation of ET‐26 hydrochloride, a novel intravenous anesthetic agent, in beagle dogs
Authors: Zhang YuJun , Deng ChaoYi , Gong DeYing , Kang Yi , Liu Jin , Zhang WenSheng 
Published: December 2019

AbstractET‐26 hydrochloride (ET‐26HCl) is a novel etomidate analogue, approved for clinical trials, which has an effective sedative‐hypnotic effect, a stable myocardial performance, and milder adrenocortical suppression than etomidate in rats and beagle dogs. Additionally, ET‐26HCl showed similar hemodynamic stability as etomidate in the rat uncontrolled hemorrhagic shock model. Furthermore, ET‐26HCl, in the rat lipopolysaccharide‐induced sepsis model, was found to have a higher survival rate, a lower inflammatory reaction, and less organ injury. In the present study, we measured the potential adverse effects of ET‐26HCl in beagle dogs in accordance with the Guidance on single‐ and repeated‐dose toxicity published by the China Food and Drug Administration. In toxicity studies, single and repeated (14 days) intravenous doses of up to 16 mg/kg were well tolerated, with only pharmacologically related clinical signs seen in both studies. Thus, the no‐observed‐adverse‐effect level (NOAEL) of ET‐26HCl was found at 16 mg/kg/day. Toxicokinetic examination demonstrated that ET‐26HCl showed a dose‐dependent increase to exposure, no gender difference, and no evidence of accumulation. These results provide useful information for guiding a phase I clinical trial of ET‐26HCl in healthy volunteers.
A novel de novo dominant mutation of NOTCH1 gene in an Iranian family with non‐syndromic congenital heart disease
Authors: Kalayinia Samira , Maleki Majid , Mahdavi Mohammad , Mahdieh Nejat 
Published: December 2019

AbstractBackgroundCongenital heart disease (CHD) is the most common birth defect which can arises from different genetic defects. The genetic heterogeneity of this disease leads to restricted success in candidate genes screening method. Emerging approaches such as next‐generation sequencing (NGS)‐based genetic analysis might provide a better understating of CHD etiology in the patients who are left undiagnosed. To this aim, in this study, we survived the causes of CHD in an Iranian family who was consanguineous and had two affected children.MethodsAffected individuals of this family were checked previously by PCR‐direct sequencing for six candidate genes (NKX2‐5, ZIC3, NODAL, FOXH1, GJA1, GATA4) and had not revealed any reported CHD causative mutations. Whole‐exome sequencing (WES) was performed on this family probond to determine the underlying cause of CHD, and the identified variants were confirmed and segregated by Sanger sequencing.ResultsWe identified one heterozygous missense mutation, c.T6797C (p.Phe2266Ser), in the NOTCH1 gene, which seems to be the most probably disease causing of this family patients. This mutation was found to be novel and not reported on 1000 Genomes Project, dbSNP, and ExAC.ConclusionWorldwide, mutations in NOTCH1 gene are considered as one of the most known causes of CHD. The found NOTCH1 variant in this family affected individuals was the first report from Iran. Yet again, this result indicates the importance of NOTCH1 screening in CHD patients.
Values of 5mC, 5hmC, and TET2 for identifying the presence and progression of breast precancerous lesion
Authors: Zhang Zijing , Jin Yiting , Zhang Wei , Chu Chengyu , Zhang Ke , Gao Xingyan , Zhou Jian , Zou Liping , Tang Feng , Wang Hongying , Zou Qiang 
Published: December 2019

AbstractBackgroundThis study aimed to evaluate the correlations of 5‐methylcytosine (5mC), 5‐hydroxymethylcytosine (5hmC), and ten‐eleven translocation enzyme 2 (TET2) expressions in lesion tissue with histological classification of breast precancerous lesion.MethodsEighty‐three patients with breast ductal intraepithelial neoplasia (DIN), 20 patients with breast ductal carcinoma in situ with microinvasion (DCIS‐MI), and 10 patients with invasive breast cancer were included. Histological classification of the DIN patients was classified as DIN1A, DIN1B, DIN1C, DIN2, and DIN3. 5mC, 5hmC, and TET2 expressions in lesion tissues from biopsy were assessed by immunohistochemistry (IHC) assay.Results5hmC and TET2 were negatively associated with histological classification as validated by both IHC score and IHC semi‐quantification expression grades in total patients (all P < .05); however, no correlation of 5mC with histological classification was found (all P > .05). 5mC (P = .004) was negatively but 5hmC (P < .001) was positively correlated with TET2, while no association of 5mC with 5hmC was discovered in total patients (P = .078). In addition, 5mC was positively associated with ER expression in total patients (P = .040). In subgroups, 5mC was negatively correlated with 5hmC in DIN1C patients (P = .023) and invasive cancer patients (P = .044), and 5mC was negatively associated with TET2 in DIN1B patients (P = .004) as well as DCIS‐MI patients (P = .003).Conclusion5hmC and TET2 have the potentials to serve as biomarkers that could assist in the identification of presence and progression of breast precancerous lesion.
The epigenetic reader Brd4 is required for osteoblast differentiation
Authors: Paradise Christopher R. , Galvan M. Lizeth , Kubrova Eva , Bowden Sierra , Liu Esther , Carstens Mason F. , Thaler Roman , Stein Gary S. , van Wijnen Andre J. , Dudakovic Amel 
Published: December 2019

AbstractTranscription networks and epigenetic mechanisms including DNA methylation, histone modifications, and noncoding RNAs control lineage commitment of multipotent mesenchymal progenitor cells. Proteins that read, write, and erase histone tail modifications curate and interpret the highly intricate histone code. Epigenetic reader proteins that recognize and bind histone marks provide a crucial link between histone modifications and their downstream biological effects. Here, we investigate the role of bromodomain‐containing (BRD) proteins, which recognize acetylated histones, during osteogenic differentiation. Using RNA‐sequencing (RNA‐seq) analysis, we screened for BRD proteins (n = 40) that are robustly expressed in MC3T3 osteoblasts. We focused functional follow‐up studies on Brd2 and Brd4 which are highly expressed in MC3T3 preosteoblasts and represent “bromodomain and extra terminal domain” (BET) proteins that are sensitive to pharmacological agents (BET inhibitors). We show that small interfering RNA depletion of Brd4 has stronger inhibitory effects on osteoblast differentiation than Brd2 loss as measured by osteoblast‐related gene expression, extracellular matrix deposition, and alkaline phosphatase activity. Similar effects on osteoblast differentiation are seen with the BET inhibitor +JQ1, and this effect is reversible upon its removal indicating that this small molecule has no lasting effects on the differentiation capacity of MC3T3 cells. Mechanistically, we find that Brd4 binds at known Runx2 binding sites in promoters of bone‐related genes. Collectively, these findings suggest that Brd4 is recruited to osteoblast‐specific genes and may cooperate with bone‐related transcription factors to promote osteoblast lineage commitment and maturation.
Manganese oxides supported on ACFN by a one‐step redox method for the low‐temperature NOx reduction with NH3: effect of acid addition
Authors: Tang Xiaolong , Gu Tian , Yi Honghong , Zhao Shunzheng , Gao Fengyu , Zhang Runcao , Wang Yuhe 
Published: December 2019

AbstractBACKGROUNDIn this study, manganese dioxide (MnO2) was formed in situ on the surface of ACFN (nitric acid modified activated carbon fibres) by a one‐step redox method for the selective catalytic reduction (SCR) of nitric oxides (NOx) with ammonia (NH3) at low temperature. Meanwhile, the water and sulfur dioxide (H2O and SO2) deactivation mechanism were investigated.RESULTSEach acid, including hydrochloric, nitric and acetic (HCl, HNO3 and CH3COOH), released H+ in solution at different rates, which lead to distinct pH environments. As the driving force, it has an effect on the redox reaction, resulting in the different MnO2 amount and performance of the MnO2/ACFN catalysts. The MnO2/ACFN (CH3COOH) catalyst exhibited good NH3‐SCR activity, water resistance and sulfur resistance.CONCLUSIONThe MnO2/ACFN (CH3COOH) catalyst showed 3D growth and uniform distribution of MnO2 sheets on the surface of the support, which resulted in a larger specific surface area and better redox properties. The CH3COOH can gradually decompose H+ so that H+ is given priority to internal diffusion. The redox reaction preferentially took place in the interior, thereby promoting the formation of more MnO2, which is beneficial to the NH3‐SCR reaction. © 2019 Society of Chemical Industry
Development of a Novel Multiparametric MRI Radiomic Nomogram for Preoperative Evaluation of Early Recurrence in Resectable Pancreatic Cancer
Authors: Tang Tian‐Yu , Li Xiang , Zhang Qi , Guo Cheng‐Xiang , Zhang Xiao‐Zhen , Lao Meng‐Yi , Shen Yi‐Nan , Xiao Wen‐Bo , Ying Shi‐Hong , Sun Ke , Yu Ri‐Sheng , Gao Shun‐Liang , Que Ri‐Sheng , Chen Wei , Huang Da‐Bing , Pang Pei‐Pei , Bai Xue‐Li , Liang Ting‐Bo 
Published: December 2019

BackgroundIn pancreatic cancer, methods to predict early recurrence (ER) and identify patients at increased risk of relapse are urgently required.PurposeTo develop a radiomic nomogram based on MR radiomics to stratify patients preoperatively and potentially improve clinical practice.Study TypeRetrospective.PopulationWe enrolled 303 patients from two medical centers. Patients with a disease‐free survival ≤12 months were assigned as the ER group (n = 130). Patients from the first medical center were divided into a training cohort (n = 123) and an internal validation cohort (n = 54). Patients from the second medical center were used as the external independent validation cohort (n = 126).Field Strength/Sequence3.0T axial T1‐weighted (T1‐w), T2‐weighted (T2‐w), contrast‐enhanced T1‐weighted (CET1‐w).AssessmentER was confirmed via imaging studies as MRI or CT. Risk factors, including clinical stage, CA19‐9, and radiomic‐related features of ER were assessed. In addition, to determine the intra‐ and interobserver reproducibility of radiomic features extraction, the intra‐ and interclass correlation coefficients (ICC) were calculated.Statistical TestsThe area under the receiver‐operator characteristic (ROC) curve (AUC) was used to evaluate the predictive accuracy of the radiomic signature in both the training and test groups. The results of decision curve analysis (DCA) indicated that the radiomic nomogram achieved the most net benefit.ResultsThe AUC values of ER evaluation for the radiomics signature were 0.80 (training cohort), 0.81 (internal validation cohort), and 0.78 (external validation cohort). Multivariate logistic analysis identified the radiomic signature, CA19‐9 level, and clinical stage as independent parameters of ER. A radiomic nomogram was then developed incorporating the CA19‐9 level and clinical stage. The AUC values for ER risk evaluation using the radiomic nomogram were 0.87 (training cohort), 0.88 (internal validation cohort), and 0.85 (external validation cohort).Data ConclusionThe radiomic nomogram can effectively evaluate ER risks in patients with resectable pancreatic cancer preoperatively, which could potentially improve treatment strategies and facilitate personalized therapy in pancreatic cancer.Level of Evidence: 4Technical Efficacy: Stage 4J. Magn. Reson. Imaging 2020;52:231–245.
Characterization of synovial fluid from periprosthetic infection in revision total joint arthroplasty by single‐molecule microscopy
Authors: Kohlhof Hendrik , Fröschen Frank S. , Randau Thomas M. , Hischebeth Gunnar , Kehrer Michael , Wirtz Dieter C. , Schildberg Frank A. , Kaminski Tim P. 
Published: December 2019

AbstractPeriprosthetic joint infection is among the most common and severe complications in total joint arthroplasty. Today, a combination of different methods is used for diagnosis because no single method with sufficient sensitivity and specificity is available. In this study, we explored the usability of single‐molecule microscopy to characterize synovial fluid samples from periprosthetic joint infections. Patients (n = 27) that needed revision arthroplasty underwent the routine diagnostic procedures for periprosthetic joint infection of the University Hospital in Bonn. Additionally, the diffusion rate of two probes, dextran and hyaluronan, was measured in small volumes of periprosthetic synovial fluid samples using single‐molecule microscopy. To evaluate the suitability of single‐molecule microscopy to detect PJI the AUC for both markers was calculated. The diffusion rate of hyaluronan in periprosthetic synovial fluid from patients with septic loosening was faster than in samples from patients with aseptic loosening. Single‐molecule microscopy showed excellent diagnostic performance, with an area under the receiver operating characteristic curve of 0.93, and allowed the detection of periprosthetic joint infection in patients that would be challenging to diagnose with current methods. For the first time, single‐molecule microscopy was used to detect periprosthetic joint infection. Our results are encouraging to study the value of single‐molecule microscopy in a larger patient cohort. The speed and accuracy of single‐molecule microscopy can be used to further characterize synovial fluid, potentially allowing intraoperative diagnosis of periprosthetic joint infections in the future.
Combination of high‐resolution accurate mass spectrometry and network pharmacology provides a new method for the chemical constituents’ study and target prediction in vivo of Garcinia multiflora
Authors: Li Zhuolun , Zuo Lihua , Shi Yingying , Tian Dongsong , Liu Liwei , Yang Yantao , Zhou Lin , Zhang Xiaojian , Kang Jian , Hao Xiaojiang , Yuan Chunmao , Sun Zhi 
Published: December 2019

AbstractGarcinia multiflora is a kind of evergreen tree which is widely distributed in the south of China. However, few researches focused on the constituents in different parts of G. multiflora as well as their potential targets and pathways in vivo. To clarify the chemical constituents of G. multiflora rapidly and predict the potential targets as well as pathways in vivo that this plant may have effects on, a feasible and accurate strategy was developed to identify the chemical constituents in fruits, leaves, and branches of G. multiflora by ultra‐high performance liquid chromatography with Q‐Exactive hybrid quadrupole‐orbitrap high‐resolution accurate mass spectrometry. Network pharmacology was then employed and a “compounds‐targets‐diseases” network was established. Sixty‐one compounds including polycyclic polyprenylated acylphloroglucinols, xanthones, and flavonoids were finally identified in different parts of G. multiflora, and the contents of seven constituents were quantified, respectively. On the basis of the network pharmacology analysis results, compounds in this plant were speculated to have potential pharmacodynamic effect on cancer, inflammatory, respiratory diseases, cardiovascular diseases, and metabolic diseases. This research will provide a new method for the advanced study on the pharmacodynamic materials basis of G. multiflora, and offer valuable evidences for medicinal purpose of this plant.
Deep eutectic solvents skeleton typed molecularly imprinted chitosan microsphere coated magnetic graphene oxide for solid‐phase microextraction of chlorophenols from environmental water
Authors: Li Guizhen , Row Kyung Ho 
Published: December 2019

AbstractNovel molecularly imprinted chitosan microspheres were prepared on the surface of magnetic graphene oxide, with deep eutectic solvents both as a functional monomer and template. The prepared molecularly imprinted chitosan microspheres‐magnetic graphene oxide was characterized by scanning electron microscopy, Fourier transform infrared spectroscopy, X‐ray diffraction, Brunauer‐Emmett‐Teller surface area, thermogravimetric analysis were subsequently combined with solid‐phase micro‐extraction for simultaneous separation and enrichment of the extraction of chlorophenols from environmental water. Factors affecting the extraction efficiency of chlorophenols were optimized using response surface methodology. The actual extraction capacities under the optimal conditions (liquid to solid ratio = 3, cycles of adsorption/desorption = 5, 40°C extraction temperature, and extraction time for 35 min) were 86.90 mg/g. Compared to the traditional materials, the molecularly imprinted chitosan microspheres‐magnetic graphene oxide produced higher selectivity and extraction capacity.
Speed of Sound and Attenuation Temperature Dependence of Bovine Brain: Ex Vivo Study
Authors: Dahis Daniel , Azhari Haim 
Published: December 2019

ObjectivesBrain treatments using focused ultrasound (FUS) offer a new range of noninvasive transcranial therapies. The acoustic energy deposition during these procedures may induce a temperature elevation in the tissue; therefore, noninvasive thermal monitoring is essential. Magnetic resonance imaging is the current adopted monitoring modality, but its high operational costs and limited availability may hinder the accessibility to FUS treatments. Aiming at the development of a thermometric ultrasound (US) method for the brain, the specific objective of this investigation was to study the acoustic thermal response of the speed of sound (SOS) and attenuation coefficient (AC) of different brain tissues: namely white matter (WM) and cortical matter.MethodsSixteen ex vivo bovine brain samples were investigated. These included 7 WM and 9 cortical matter samples. The samples were gradually heated to about 45°C and then repeatedly scanned while cooling using a computerized US system in the through‐transmission mode. The temperature was simultaneously registered with thermocouples. From the scans, the normalized SOS and AC for both tissues were calculated.ResultsThe results demonstrated a characteristic cooldown temporal behavior for the normalized AC and SOS curves, which were related to the temperature. The SOS curves enabled clear differentiation between the tissue types but depicted more scattered trajectories for the WM tissue. As for the AC curves, the WM depicted a linear behavior in relation to the temperature. However, both tissue types had rather similar temperature patterns.ConclusionsThese findings may contribute to the development of a US temperature‐monitoring method during FUS procedures.
FKBP10 functioned as a cancer‐promoting factor mediates cell proliferation, invasion, and migration via regulating PI3K signaling pathway in stomach adenocarcinoma
Authors: Wang Ruo‐Gu , Zhang Dan , Zhao Chun‐Hong , Wang Qi‐Long , Qu Hui , He Qing‐Si 
Published: December 2019

AbstractAs documented, the expression, biological roles, and prognostic significance of FKBP10 in stomach adenocarcinoma (STAD) have not been investigated till now. This drives us to detect the biological roles and clinical significance of FKBP10 in STAD. The expression level of FKBP10 was measured based on the data obtained from the TCGA, ONCOMINE, and GEPIA databases, and STAD cell lines. Through in vitro experiments, cell behaviors were investigated to evaluate the effects of FKBP10 on STAD. Moreover, the PI3K‐AKT signaling pathway was measured. Relying on the data of TCGA, ONCOMINE, and GEPIA databases, and cancer cell lines, FKBP10 was up‐regulated in STAD when compared with normals. The patients with low expression of FKBP10 had higher survival rate than those with high FKBP10 expression. After knockdown of FKBP10 in AGS cells, cell vitality, colony formation ability, and the migratory and invasive potential were inhibited. Western blotting analysis exhibited that knockdown of FKBP10 significantly reduced the expression level of p‐AKT, and p‐PI3K, but it did not influence the total expression level of AKT, and PI3K. FKBP10 might serve as a crucial player in gastric cancer, and targeting FKBP10 might provide clinical utility in gastric cancer in future.