bluuebunny/crossref_metadata_2025_split huggingface dataset:
Needs, Aggravation, And Degree Of Burnout In Informal Care Providers Of Patients With Chronic Cvd
Authors: Szlenk-Czyczerska Elżbieta , Guzik Marika , Bielska Dorota Emilia , Ławnik Anna , Polański Piotr , Kurpas Donata 
Published: March 2020

Abstract
Abstract
Background. A growing number of patients with chronic cardiovascular disease (CVD) creates a growing demand for homecare. Personal and professional limitations, health issues, and lack of systemic support of informal care providers are major causes of insufficient care models for chronically ill patients in home environments. This study aimed to identify the sociodemographic variables that are associated with the needs and increasing occupational burnout observed among home care providers.
Methods. This study reports on 161 informal home care providers of patients with CVDs. The research was conducted in the homes of patients, using the Camberwell Assessment of Need Short Appraisal Schedule (CANSAS), the Maslach Burnout Inventory (MBI), and a structured interview questionnaire developed by the authors. Spearman’s rank correlation coefficient test and logistic regression were used for analyses.
Results. The majority of the participants were female (70.2%; n=113). We found that younger care providers were less likely to report unmet needs ( p = 0.011), and less likely to report burnout as measured by the MBI Emotional Exhaustion ( p = 0.010) and Depersonalization ( p = 0.009) subscales. Care providers with primary education were more likely to report burnout on the MBI Depersonalization subscale ( p = 0.028). In addition, care providers who worked more often reported higher levels of met needs ( p = 0.022), and burnout as measured on the MBI Depersonalization ( p = 0.005) and Emotional Exhaustion ( p = 0.018) subscales. Subjects residing in urban areas were more likely to report unmet needs ( p = 0.007), and were also more likely to report burnout as measured on the MBI Emotional Exhaustion ( p = 0.006) subscale.
Conclusion. Older care providers who are unemployed and reside in cities and unemployed should be targeted with directed programmes to determine the category of unmet needs, and for personalized support. Care providers with these demographic characteristics should be targeted for occupational burnout prevention programmes.
Keywords: informal caretaker, met and unmet needs, growing burnout.
lncRNA CRNDE promotes the proliferation and metastasis by acting as sponge miR-539-p to regulate POU2F1 expression in HCC
Authors: Li Zhixi , Wu Gang , Li Jie , Wang Youyu , Ju Xueming , Jiang Wenjun 
Published: March 2020

Abstract
Background This article focuses on the roles and mechanism of lncRNA CRNDE on the progression of HCC. Methods We used qRT-PCR to detect the expression of lncRNA CRNDE in HCC cells, normal cells and clinical tissues. MTT assay, FCM analysis, Transwell migration and invasion assay were used to detect the effects of lncRNA CRNDE on cell viability, apoptosis, migration and invasion of HCC cells. The expression of apoptosis-related proteins Bcl-2, Bax, Cleaved Caspase 3, Cleaved Caspase 9, EMT epithelial marker E-cadherin and mesothelial marker Vimentin were analyzed by Western blot. Online prediction software was used to predict the binding sites between lncRNA CRNDE and miR-539-5p, or miR-539-5p and POU2F1 3’UTR. Dual luciferase reporter assay, qRT-PCR and RNA pulldown were used to detect target-relationship between lncRNA CRNDE and miR-539-5p. Dual luciferase reporter assay, qRT-PCR, Western blot and Immunofluorescence were used to detect target-relationship between miR-539-5p and POU2F1. qRT-PCR was used to detect the expression of miR-539-5p and POU2F1 in clinical tissues. Rescue experiments was used to evaluate the association among lncRNA CRNDE, miR-539-5p and POU2F1. Finally, we used Western blot to detect the effects of lncRNA CRNDE, miR-539-5p and POU2F1 on NF-κB and AKT pathway. Results lncRNA CRNDE was highly expressed in HCC cells and HCC tissues compared with normal cells and the corresponding adjacent normal tissues. lncRNA CRNDE promoted the cell viability, migration and invasion of HCC cells, while inhibited the apoptosis and promoted the EMT process of HCC cells. lncRNA CRNDE adsorbed miR-539-5p acts as a competitive endogenous RNA to regulate POU2F1 expression indirectly. In HCC clinical tissues, miR-539-5p expression decreased and POU2F1 increased compared with the corresponding adjacent normal tissues. lncRNA CRNDE/miR-539-5p/POU2-
F1 participated the NF-κB and AKT pathway in HCC. Conclusion lncRNA CRNDE promotes the expression of POU2F1 by adsorbing miR-539-5p, thus promoting the progression of HCC.
Keywords: HCC, lncRNA CRNDE, miR-539-5p, POU2F1, ceRNA
Comparison of the effectiveness of Aloe Vera Gel with 2% Nitrofurazone ointment on the healing of superficial second-degree burns. Randomised clinical trial
Authors: Varaei Shokoh , Ranjbar Hadi , Sabaghzadeh Parichehr , Bostani Sanaz , Amirsalari Soodabhe 
Published: March 2020

Abstract
Background Burn injuries are one of the most common sources of trauma globally that comprise a significant drain on long-term personal and healthcare cost Large surface area burn wounds are difficult to manage and may result in significant physiologic and psychological sequelae . The aim of this study was to compare the effectiveness of Aloe Vera gel with Nitrofurazone ointment in the healing of superficial second-degree burn wounds.Methods The present study was a split body controlled, randomized clinical trial. The sample was recruited from patients with superficial second-degree burn wound who were prescribed to treat with 2% Nitrofurazone ointment. Thirty patients with at least two burn, each burn on an alternate side of the body, entered the study — samples allocated to two groups which received Aloe Vera gel or 2% Nitrofurazone ointment on their burns. Bates-Jensen Wound assessment tool (BWAT) was used to evaluate the healing of burns. The burns were evaluated before, one, two and three weeks after the beginning of treatment.Results The mean ± SD of BWAT scores in Aloe Vera zones were 30.32 ± 3.28, 27.33 ± 3.38, 21.33 ± 3.13, 16.12 ± 2.16 respectively (F(2, 65.07) =440.00, p=0.001). The mean ± SD of BWAT scores in Nitrofurazone ointment zones were 30.51 ± 3.79, 28.45 ± 3.49, 23.36 ± 2.89, 19.23 ± 2.11 (F(1, 52.00) =228.00, p=0.001).Conclusions There is a significant difference in (BWAT) scores between intervention and control groups. Aloe Vera gel was as effective as Nitrofurazone ointment in the treatment of superficial second-degree burns.Trial registration IRCT2014113020151N1. Registered 14 December 2014, https://www.irct.ir/trial/17874
Symptoms of ocular surface disease in construction workers: comparative study with office workers.
Authors: Hernandez-Llamas Sergio , Paz-Ramos Ana Karen , Marcos-González Patricio , Amparo Francisco , Garza Manuel 
Published: March 2020

Abstract
Purpose To investigate and contrast the prevalence of dry eye symptoms in construction workers and office workers using the OSDI questionnaire. Methods A cross-sectional, observational study was conducted using the OSDI questionnaire to evaluate dry eye symptoms and associated risk factors. Sampled size calculation with a power of 80% and a 95% degree of confidence suggested the inclusion of 298 participants. Results We studied 304 subjects (149 construction workers and 155 office workers). More than half (55%) of the participants presented dry eye symptoms (OSDI > 12). The average OSDI score was 21.30 ± 22.20 points, being lower in the group of construction workers (12.45±17.50) than in-office workers (28.51±22.99) (p <0.001). Considering participants who had moderate and severe symptoms (23 to 100 points in OSDI), office workers presented dry eye symptoms 4.15 times more frequent than construction workers (OR 4.15, 95% CI 2.52, 6.85). Women presented statistical evidence of higher OSDI scores than men (32.47 ± 23.72 vs. 14.87 ± 18.48, respectively). Conclusions Construction workers have four times less risk of presenting dry eye symptoms than people working in the average office space highlighting the pernicious effects of some of the conditions in the office environment to the ocular surface. .
Evolution of arthroscopic treatment from intracapsular to extracapsular for patients with refractory tennis elbow: A mixed-method study.
Authors: Yang Yu-ping , Chu Hong-ling , Yuan Shuo , Yan Hui , Xiao Jian , Cheng Xu , Luo Hao , Liu Yu-lei , Tao Li-yuan , Zhao Yi-ming , Cui Guoqing 
Published: March 2020

Abstract
Background Arthroscopic surgery is widely used in the treatment of refractory tennis elbow due to its advantages of minimally invasive, safe and direct vision. Intra-capsular arthroscopy(intracapsular method), which is performed via natural cavity, is the mainstream arthroscopic procedure. However, intracapsular method requires the damage of joint capsule and may cause intraoperative neurovascular injury and other complications. In view of the defects of intracapsular method, the surgical scheme of the extracapsular arthroscopy (referred to as "extracapsular method") has emerged. The aim of this study was to investigate the exploration history, key theories, methods, and techniques involved in the evolution from the intracapsular method to the extracapsular method for arthroscopic treatment of tennis elbow, and explore the effectiveness and safety of extracapsular method. Methods Qualitative data were collected via focus group interview. 7 surgeons who were selected through purposive sampling discussed on the theories, methods, and technical specifications of the transition from intracapsular method to extracapsular method. Qualitative data were analysed using NVivo11. Quantitative data of consecutive 43 patients were analysed to evaluate the effectiveness and safety of the extracapsular method. Descriptive analysis was conducted to analyse the demographic characteristics and clinical outcomes after surgery. Results The experts agreed that the extracapsular method maintains the integrity of joint capsule and avoids complications caused by joint capsule damage. Extracapsular method was considered easy for surgeons to master and convenient to operate. Technical specifications for extracapsular method were formed based on group discussion. The median time for pain relief and recovery of daily activities of 43 patients treated with these specifications were 3 months and 6 weeks, respectively. The satisfaction rates of Mayo Elbow Performance Scores were 87.2%, 95.6%, and 100% respectively at 3, 6, and 12 months after operation. Conclusion On the basis of international peer innovation, the extracapsular method has been continuously developed in our clinical practice to avoid the possible complications of the intracapsular method. The resulting technical specifications have been shown to have good clinical efficacy and safety in a small sample cohort.
Establishment and validation of finite element model of ossification of cervical posterior longitudinal ligament with intervertebral fusion
Authors: Hui Li , Huiqing Liu , Yaning Zhang 
Published: March 2020

Abstract
[Background ]: To establish a three-dimensional finite element model of ossification of the posterior longitudinal ligament of the cervical spine with intervertebral fusion and verify its effectiveness, and provide a platform for finite element calculation and biomechanical analysis in the later stage.[Method]: Select the Department of Spinal Surgery, Linfen People's Hospital A volunteer imported 719 DICOM format images of cervical spine CT scans into Mimics modeling software to build a preliminary 3D model in the stl format, and used Geomagic Studio 2013 software to refine and refine the 3D model to smooth out noise and generate NURBS surfaces The model was then imported into the finite element analysis software Ansys workbench 15.0, adding ligaments and intervertebral discs, meshing, assigning material properties, and simulating 6 activities of the human cervical spine, and comparing them with references.[Results]: A total of 7 Cervical vertebral body, 1 thoracic vertebral body, 5 intervertebral discs and ligaments, etc., with a total of 320512 nodes and 180905 units. It has a realistic appearance, high degree of detail reduction, and ossification of the cervical longitudinal longitudinal ligament with good geometric similarity Incorporate a three-dimensional finite element model of intervertebral fusion. In flexion and extension, left and right lateral flexion, and axial rotation activity compared with references, there is not much difference.[Conclusion]: OPLL merger interbody fusion dimensional finite element model has good mechanical and geometric similarity after similarity cervical established in this study, the model can provide a platform for the latter to further biomechanical analysis.
Gradient engineered light absorption layer for enhanced carrier separation efficiency in perovskite solar cells
Authors: Wu Gaozhu , Zhu Qing , Zhang Teng , Zou Ziqi , Wang Weiping , Cao Yiyan , Kong Lijing , Zheng Xuanli , Wu Yaping , Li Xu , Wu Zhiming , Kang Junyong 
Published: March 2020

Abstract
Carrier transport behavior in perovskite light absorption layer significantly impacts the performance of perovskite solar cells (PSCs). In this work, reduced carrier recombination losses were achieved by the design of band structure in perovskite materials. An ultrathin (PbI 2 /PbBr 2 ) n film with a gradient thickness ratio was deposited as the lead halide precursor layer by thermal evaporation method, and PSCs with a gradient band structure in perovskite absorption layer were fabricated by a two-step method in ambient atmosphere. For comparison, PSCs with homogeneous perovskite materials of MAPbI 3 and MAPbI x Br 3-x were fabricated as well. It is found that the gradient type-II band structure greatly reduces the carrier lifetime and enhances the carrier separation efficiency. As a result, the PSCs with a gradient band structure exhibit an average power conversion efficiency of 17.5%, which is 1-2% higher than that of traditional PSCs. This work provides a novel method for developing high-efficient PSCs.
Preterm Intraventricular Hemorrhage in vitro: Modeling the Cytopathology of the Ventricular Zone
Authors: Castaneyra-Ruiz Leandro , McAllister James P. , Morales Diego M. , Brody Steven L. , Isaacs Albert M. , Limbrick David D. 
Published: March 2020

Abstract
Background: Severe intraventricular hemorrhage (IVH) is one of the most devastating neurological complications in preterm infants, with the majority suffering long-term neurological morbidity and up to 50 percent developing post hemorrhagic hydrocephalus (PHH). Despite the importance of this disease, its cytopathological mechanisms are not well known. An in vitro model of IVH is required to investigate the effects of blood and its components on the developing ventricular zone (VZ) and its stem cell niche. To address this need, we developed a new in vitro model to mimic the cytopathological conditions of IVH in the preterm infant. Methods: Maturing neuroepithelial cells from the VZ were harvested from the entire lateral ventricles of wild type C57BL/6 mice at 1-4 days of age and expanded in proliferation media for 3-5 days. At confluence, cells were re-plated onto 24-well plates in differentiation media to generate ependymal cells (EC). At approximately 3-5 days, which corresponded to the onset of ependymal cell differentiation based on the appearance of multiciliated cells , phosphate-buffered saline for controls or syngeneic whole blood for IVH was added to the ependymal cell surface. The cells were examined for the expression of EC markers of differentiation and maturation to qualitatively and quantitatively assess the effect of blood exposure on VZ transition from neuroepithelial cells to EC. Discussion: This model will allow investigators to test cytopathological mechanisms contributing to the pathology of IVH with high temporal resolution and query the impact of injury to the maturation of the VZ. This technique recapitulates features of normal maturation of the VZ in vitro, offering the capacity to investigate the developmental features of VZ biogenesis.
Physical activity intervention for rural middle-aged and older Australian adults: A pilot implementation study of the ecofit program delivered in a real-world setting
Authors: Wilczynska Magdalena , Jansson Anna K , Lubans David R , Smith Jordan J , Robards Sara L , Plotnikoff Ronald Cyril 
Published: March 2020

Abstract
Background: ecofit is an evidence-based multi-component physical activity intervention that integrates smartphone technology, the outdoor environment and social support. In a previous efficacy trial, significant improvements were found across several clinical, fitness, and mental health outcomes among adults at risk of (or with) type 2 diabetes. Methods: The aim of the present pilot study was to evaluate a number of health-related and feasibility outcomes of the ecofit intervention in a ‘real-world’ setting, using a scalable implementation model. ecofit was adapted and implemented by a rural municipal council in the Upper Hunter Shire, New South Wales, Australia and evaluated using a single-group pre-post design. Inactive middle-aged and older adults (N=59) were recruited and assessed at 6- (primary time-point) and 20-weeks (follow-up). Results: Improvements were found in this predominantly overweight and obese sample for aerobic fitness, functional mobility, upper and lower body muscular fitness, systolic blood pressure and waist circumference at 6-weeks. At 20-weeks, effects were found for aerobic fitness, functional mobility, upper and lower body muscular fitness, and systolic blood pressure. Overall, participants were satisfied with the ecofit program. Participants attended the 6-week primary time-point (66.1%) and follow-up at 20-weeks (41.6%). Conclusions: Our findings support the preliminary effectiveness and feasibility of the ecofit intervention delivered by municipal council staff following a brief training from the research team. This study provides valuable preliminary evidence to support of a larger implementation trial.
Identification of the molecular mechanism and diagnostic biomarkers in the thoracic ossification of the ligamentum flavum using metabolomics and transcriptomics
Authors: Li Jiahao , Yu Lingjia , Guo Shigong , Zhao Yu 
Published: March 2020

Abstract
Background: To establish a metabolite fingerprint of ossification of the thoracic ligamentum flavum (OTLF) patients using liquid chromatography-mass spectrometry (LC-MS) in combination with transcriptomic data and explore the potential molecular mechanism of pathogenesis. Results: 37 metabolites were identifed out by UPLC-MS including uric acid and hypoxanthine. Variable Importance in Projection (VIP) scores suggest that uric acid might have an impact on the process of OTLF. Pathway enrichment indicated that five metabolism pathways were enriched. Transcriptomic data revealed that the purine metabolism pathway may have significant impact on the process of OTLF and that xanthine dehydrogenase (XDH) is the key regulatory factor. Receiver operating characteristic (ROC) analysis indicated that uric acid might be the potential biomarker for OTLF and play an important role within the detailed pathway. ROC revealed that XDH might suppress the metabolism of hypoxanthine and xanthine, leading to a low expression of uric acid, which could be a focal point in developing new therapeutic methods for OTLF. Conclusion: XDH could affect purine metabolism by suppressing the expression of hypoxanthine and xanthine leading to low serum levels of uric acid in OTLF.
Prevalence, antimicrobial susceptibility and genotypic characteristics of Staphylococcus aureus in Tanzania: a systematic review
Authors: Mzee Tutu , Kazimoto Theckla , Madata Joseph , Masalu Rose , Bischoff Markus , Matee Mecky , Becker Sören 
Published: March 2020

Abstract
Background : Information about prevalence, antibiotic resistance and genotyping data of Staphylococcus aureus (S aureus) isolated from different infections and colonization, both in humans and animals is scarce in Tanzania. Given the wide range of infections S aureus can cause and the raise of methicillin resistant S aureus (MRSA) globally, this review was aimed at collecting local published data on the S aureus bacterium to better understand the epidemiology. Methodology: A systematic review of scientific studies reporting on prevalence, antibiotic resistance and genotyping of S aureus in human and animal infection and colonization was done. Literatures extracted from electronic databases such as PubMed and Google Scholar were screened for eligibility and relevant articles included into the review. The review is limited to literatures published in English, between the years 2010 and 2018. . Results: 39 studies conducted in 5 of the 9 administrative zones in Tanzania were reviewed to establish S aureus prevalence in human and animal infection and colonization. Prevalence in human ranged from 1 - 60%. Antibiotic resistance patterns of S aureus isolated from colonized humans showed higher resistance rates against erythromycin (49%), co-trimoxazole (38%) and tetracycline (28%) compared to report from other studies outside Africa. The review suggests an increased MRSA prevalence of up to 33% compared to 16% reported in previous years. Genotypic data reviewed suggested that MRSA predominantly belonged to ST88. S aureus prevalence in animal studies ranged from 33 - 49% whereas MRSA prevalence ranged from 4 - 35%. Generally low antibiotic resistance levels were reported in these studies with exception to penicillin (85%) and ampicillin (73%). Resistance against tetracycline reported at 30% collaborates the suggested overuse of the drug in livestock keeping in Tanzania. Conclusion : Prevalence of MRSA in Tanzania is rising although clear variations between different geographic areas could be observed. Non-susceptibility to commonly prescribed antibiotics in community associated S aureus is of concern. Research strategies to better understand the S aureus epidemiology by including genotypic characterisations, as well as strengthening antimicrobial resistance surveillance and antimicrobial stewardship are recommended.