bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of maternal dietary supplementation with Brazil nut (Bertholletia excelsa) on the nutritional profile of human milk: randomized controlled trial - rationale, design, and methods
Authors: Rodrigues Denise , Reis Roberta Sena , Correa Kely de Paula , Franceschini Sylvia do Carmo Castro , Coimbra Jane Selia dos Reis 
Published: May 2020

Abstract
Background The human milk (HM) composition reflects the nutritional status and dietary intake of the nursing mother, especially regarding the fatty acids (FA) intakes. Evidence has shown that Brazil nut is a good source of FA (mono and polyunsaturated) and selenium. Thus, this study tests the hypothesis that the consumption of this nut during lactation can modify the nutritional profile of HM, increase its energy density and selenium content, and improve the lipid profile. Therefore, this study aimed to describe the design and methods of the Randomized Clinical Trial (RCT): Effect of maternal dietary supplementation with Brazil nut (Bertholletia excelsa) on the nutritional profile of HM. Method/Design It is a crossover study, conducted with mother-infant dyads (with infants born at full-term) recruited from a maternity hospital and followed up to the third month postpartum in a pediatric office. Sociodemographic, lifestyle, obstetric, and birth data were collected at 7 days postpartum. Maternal nutrition assessment data (anthropometry, body composition, food intake, biochemical tests), HM samples, and infant anthropometric and health assessment data were collected on average at 37, 52, 75, and 90 days postpartum. The nursing mothers were randomly allocated into two groups: group (1) started with supplementation of 1.5 g/day Brazil nut (case) for 15 days, followed by 15-day washout period; after the washout, the group was then considered as control and received follow-up monitoring for another 15 days; group (2) started as control for 15 days, which was followed by a washout period of 15 days, then they initiated supplementation with Brazil nut for 15 days (case).Discussion To the best of our knowledge, this is the first RCT to evaluate the effect of maternal Brazil nut supplementation on HM nutritional profile. This is a promising research area that can provide findings which will contribute to improving health and nutrition of infants, especially premature infants, admitted to Neonatal Intensive Care Units.
Impact of Retrieved Lymph Nodes Count on Short-term Complications in Patients With Gastric Cancer: A Retrospective Study
Authors: Sun Feng , Liu Song , Song Peng , Zhang Chen , Liu Zhijian , Guan Wenxian , Wang Meng 
Published: May 2020

Abstract
Background:It is well established thatretrieved lymph nodes (RLNs) count were positively correlated with better overall survival in gastric cancer (GC). But little is known about the relationship between RLNs count and short-term complications after radical surgery.Methods: A total of 1487 consecutive GC patients between January 2016 and December 2018 at Nanjing Drum Tower Hospital were retrospectively analyzed. Univariate analyses were performed to elucidate the association between RLNs count and postoperative complications. We further identified clinical factors that might affect the RLNs count.Results: Among all of the patients, postoperative complications occurred in 435 (29.3%) patients. The mean RLNs count was 25.1 and 864 (58.1%) patients were diagnosed with lymph node metastasis. Univariate analyses showed no significant difference between RLNscount and postoperative complications (both overall and stratified by CDC grade). We further explored that preoperative serum albumin, type of resection, operation time, tumor invasion, lymph node metastasis, and pTNM stage were associated with RLNs count.Conclusions: The current study demonstrated that RLNs count was not associated with postoperative short-term complications following gastrectomy of GC, which provided a rationale for the determination of a proper RLNs count of curative gastrectomy.
Screening of postpartum depression among new mothers in Istanbul: a psychometric evaluation of the Turkish Edinburgh Postnatal Depression Scale
Authors: Boran Perran , Waqas Ahmed , Askan Öykü Ozboru , Topçu İrem , Dogan Tugay , Rahman Atif 
Published: May 2020

Abstract
Objective This study is the first concerted effort to ascertain factor structure of EPDS using evidence based analytical techniques. It is the most widely used scale for assessing postpartum depression in Turkey, and yet no investigations have been conducted to assess it factor structure. This study was conducted from April 2012 to April 2018 at the Marmara University Hospital operating under the name of Marmara University Pendik Training and Research Hospital in Istanbul Turkey. Results A total of 1700 women were included in this study, who responded to the EPDS, in addition to demographic characteristics and well-being of their offspring. A total of 1615 mothers provided adequate data for inclusion in analysis. Standardized Chronbach’s alpha for EPDS was 0.81 with corrected item-total correlations ranging from 0.35 to 0.62. Parallel analysis, MAP Velicer Test and Hull’s method dictated retaining of one factor structure. All the items revealed adequate communalities (> 0.20) except item 2 (enjoyment) and item 10 (self-harm). Their communalities were 0.16 and 0.19, however, these items were not dropped. All of the items yielded moderate to strong factor loadings. Minimum factor loading was for item 2 (0.40) and highest for item 8 (0.71).
Establishment of Reference Range of CD4 T-Lymphocyte in Healthy Nepalese Adults
Authors: Mishra Shravan Kumar , Shrestha Lilee , Pandit Roshan , Khadka Sundar , Shrestha Bimal , Dhital Subhash , Sharma Saroj , Sharma Mukunda , Mahato Raj Kumar , Shakya Geeta , Manandhar Krishna Das 
Published: May 2020

Abstract
Objective CD4 T Lymphocytes are the most widely used cellular markers to assess the course of HIV infection, clinical staging and, monitoring the effect of antiretroviral therapy. The regional reference range for Eastern, Central and Western development region of Nepal had already been established whereas the same was still lacking in Mid-western and Far-western development region. The objective of this study was to establish reference range of CD4 T Lymphocyte in the remaining two development regions and finally the national reference range using data from previous study.Results The average values (mean ± SD) of CD4 and CD3 T cell in present study was (819 ± 294) cells/µl and (1546 ± 532) cells/µl, respectively. The absolute CD4 T cell (914 ± 303) and CD3 T cell (1671 ± 560) count in female were significantly higher than those from male, CD4 (757 ± 270) and CD3 (1465 ± 499) (p value − 0.000). National reference value of CD4 was determined to be (798 ± 335) cells/µl for healthy Nepalese adults.
“I’m not a teenager, I’m 22. Why can’t I snap out of it?” A Qualitative Exploration of Seeking Help for a First-Episode Eating Disorder During Emerging Adulthood
Authors: Potterton Rachel , Austin Amelia , Allen Karina , Lawrence Vanessa , Schmidt Ulrike 
Published: May 2020

Abstract
Background Eating disorders (EDs) are serious mental illnesses which typically have their onset during adolescence or the transition to adulthood. Emerging adulthood (EA) (~ 18–25 years) is a developmental phase which conceptually overlaps with adolescence (e.g. both are key periods for identity development) but also has unique characteristics (e.g. increased independence). EA may be a risk-period for delayed help-seeking for EDs, compared to adolescence.Objective This study aimed to explore emerging adults’ attitudes towards their ED symptoms, and their implications for help-seeking. Method: Participants were 14 emerging adults (mean age 20.9 years; SD = 2.0), all currently receiving specialist treatment for a first-episode, recent-onset (< 3 years) ED. Semi-structured interviews relating to experiences of help-seeking were conducted, and data were analysed thematically.Results Symptom egosyntonicity, gradual reappraisal and stereotypical beliefs about EDs were key attitudinal stages prior to help-seeking. Each attitude had distinct implications for help-seeking (e.g. superficial help-seeking; scaffolding by others; helps-seeking at transitions; self-sufficiency, indirect disclosure).Conclusions Emerging adults with first-episode EDs show a distinct set of help-seeking-related challenges and opportunities. These may justify policies and practice tailored specifically to this population group.
EIF4A3-Mediated CircRNA_100290 Promotes GC Cell Proliferation, Invasion and EMT via miR-29b-3p/ITGA11 Axis
Authors: Wang Gang , Sun Dan , Li Wenhui , Xin Yan 
Published: May 2020

Abstract
Background Circular RNA (circRNA) has been reported as an important regulator in the development and progression of various carcinomas. However, the role of circRNA_100290 in gastric cancer (GC) is still unclear. This study aimed to investigate the role of circRNA_100290 in GC invasion and metastasis and its possible mechanism.Methods The expression of circRNA_100290 in GC cells and tissues were examined using quantitative real-time polymerase chain reaction (qRT-PCR). The role of circRNA_100290 in cell proliferation, migration, and invasion was evaluated on AGS and HGC-27 cell lines in vitro. Bioinformatics tools, dual-luciferase reporter assay, Western blot assay and qRT-PCR were used to explore the downstream pathways of circRNA_100290. The mechanism underlying the regulation of the expression of circRNA_100290 was explored using RNA immunoprecipitation, qRT-PCR, and Western blot assays.Results The expression of circRNA_100290 was found significantly upregulated in GC cells and 102 GC tissues, high expression of circRNA_100290 in GC was closely related to Borrmann’s types, lymph node metastasis and tumor-node-metastasis staging. In vitro, knockdown of circRNA_100290 in AGS and HGC-27 cells significantly inhibited cell proliferation, migration, and invasion. Mechanistically, dual-luciferase reporter assay confirmed a direct binding between circRNA_100290 and miR-29b-3p, which targets ITGA11, an oncogene which is closely related to epithelial–mesenchymal transition (EMT). In addition, EIF4A3, one of RNA binding proteins (RBPs), could inhibit the formation of circRNA_100290 via enriching flanking sites of circRNA_100290. Low expression of EIF4A3 in GC was related to a worse prognosis.Conclusions Elevated circRNA_100290 in GC promotes cell proliferation, invasion and EMT via miR-29b-3p/ITGA11 axi and might be regulated by EIF4A3. CircRNA_100290 might be a promising biomarker and target for GC therapy.
Endoscopically-controlled surgery with open hemi-laminectomy for the treatment of intradural extramedullary tumors: An operative technique and short-term outcomes of 20 consecutive cases
Authors: Yan Xiaorong , Wang Huiqing , Li Cai , Lin Yuanxiang , Lin Lin , Zhu Shinong , Wang Chenyang , Lin Zhangya , Jiang Changzhen , Kang Dezhi 
Published: May 2020

Abstract
Background
To present a surgical technique for the treatment of intradural extramedullary (IDEM) tumors by using endoscopically-controlled surgery with open hemi-laminectomy technique.
Methods
In this study, 20 patients with 22 IDEM tumors were enrolled. An endoscopically-controlled surgery with open hemi-laminectomy was employed to remove the tumors. Data related to clinical symptoms and medical images before and after surgery were collected for perioperative evaluation and follow-up analysis.
Results
All the tumors in 20 patients were well removed. The clinical symptoms were significantly reduced in all the patients as well. The short term follow-up data showed that there was no tumor recurrence or spinal deformity.
Conclusion
The endoscopically-controlled surgery with open hemi-laminectomy technique provided favorable exposure and satisfactory resection to the IDEM tumors. It may be an effective surgical method for treating IDEM tumors. Larger samples and longer follow-up data are needed to verify its long-term effectiveness.
Structure based drug discovery by virtual screening of 3699 compounds against the crystal structures of six key SARS-CoV-2 proteins
Authors: Saadat Shoab , Mansoor Salman , Naqvi Naveen , Fahim Ammad , Rehman Zaira , Khan Shahar Yar , Saadat Sidra 
Published: May 2020

Abstract
BackgroundThe current Novel Coronavirus (SARS-CoV-2) pandemic is the third major outbreak of the 21st century which emerged in December 2019 from Wuhan, China. At present there are no known treatments or vaccines to cure or prevent the illness.ObjectiveThe objective of this study was to explore a list of potential drugs (herbal and antivirals) for their role in inhibiting activity and or replication of SARS-CoV-2 by using molecular docking onto the crystal structures of key viral proteins.MethodologyIn this study, we used molecular docking to estimate the binding affinities of 3699 drugs on the potential active sites of the 6 main SARS-CoV-2 proteins (Papain like protease, Main protease, ADP Ribose phosphatase, Spike protein, NSP-9 and NSP-10 to 16 complex). While other studies have mostly been performed on the homology models, we obtained the most recently submitted crystal structures of all 6 proteins from the protein data bank for this analysis.ResultsOur results showed the top ligands as Theasinensin A, Epigallocatechin, Theaflavin, Theasinensin A, Epigallocatechin and Favipiravir showing the highest binding affinities against papain-like protease, ADP ribose phosphatase, main protease, spike protein, RNA replicase (NSP-9) and methyl-transferase (NSP-16) respectively.ConclusionWe show that the compounds from our list with the greatest inhibitory potential against SARS-CoV-2 activity or replication include Theasinensin A, Epigallocatechin-3-gallate, Theaflavin, Favipiravir, Curucumin, Quercetin, Mitoxantrone, Amentoflavone, Colistin, Cimicifugic acid, Theaflavin, Silymarin and Chebulagic. We recommend further wet-lab and clinical testing of these compounds to further explore their role against SARS-CoV-2.
The Big-Five personality traits and quality of life in elderly Malaysian patients with diabetes mellitus: A cross-sectional study
Authors: Woon Luke Sy-Cherng , Gosse Paula Junggar , Kaunismaa Emily Samantha , Mainland Roslyn Laurie , Ravindran Arun , Mustafa Norlaila , Sidi Hatta 
Published: May 2020

Abstract
Background: Diabetes mellitus is highly prevalent in the elderly population, with a significant impact on quality of life. This study aimed to explore the relationship between personality traits and quality of life in an elderly population with diabetes. Methods: A cross-sectional study was conducted at the Universiti Kebangsaan Malaysia Medical Center. Outpatients above 60 years old with a diagnosis of type 1 or type 2 diabetes mellitus were recruited. Sociodemographic and clinical information were obtained. Quality of life was assessed using the WHO Quality of Life-BREF questionnaire and personality traits were assessed using the Big Five Inventory questionnaire. Depression and anxiety were measured with Beck Depression Inventory and Generalized Anxiety Disorder 7-item scale respectively and controlled for in statistical analyses. Results: There were 170 study participants (median age=69.0 years; IQR: 65.0-73.0; 51.2% male). In stepwise linear regression models, higher conscientiousness scores (β=0.156; p=0.044) and lower neuroticism scores (β=-0.176; p=0.028) were associated with greater quality of life in the physical health domain. Higher extraversion scores (β=0.209; p=0.001) and higher conscientiousness scores (β=0.248; p<0.001) were associated with greater quality of life in the psychological health domain. Higher agreeableness scores (β=0.286; p<0.001) were associated with greater quality of life in the social relationship domain. Finally, higher agreeableness scores (β=0.327; p<0.001) and lower neuroticism scores (β=-0.223; p=0.001) were associated with greater quality of life in the environment domain. Conclusions: Personality traits were closely associated with all domains of quality of life among elderly patients with diabetes mellitus. Premorbid personality may have important role in moderating the impact of diabetes mellitus on the lives of elderly patients.
Understandings steps and challenges to take-home naloxone and buprenorphine/naloxone implementation in Québec emergency rooms: SuboxED project
Authors: Talbot Annie , Khemiri Rania , Londei-Leduc Luc , Robin Christine , Marcotte Suzanne , Therrien Guenièvre , Goulet Geneviève , Beaudet-Hillman Geneviève , Ouellette Christine , Brissette Suzanne , Sako Aïssata , Martin Marcel , Lauzon Pierre 
Published: May 2020

Abstract
Background Deaths attributable to drug abuse are on the rise across Canada. It is estimated that there were more than 13,900 opioid-related deaths from January 2016 to June 2019 in the country. Emergency departments (EDs) are often on the frontline of care provided to people at risk of opioid overdose within Québec’s healthcare system. A variety of programs to implement take-home naloxone distribution and/or the provision of opioid agonist treatment for ED patients who are at risk for overdose have been created in the United States and in Europe. However, few EDs in Canada have developed protocols for the provision of take-home naloxone and/or opioid agonist treatment by ED doctors. Methods A clinical algorithm for take home naloxone (THN) and prescription of buprenorphine/naloxone (B/N) was implemented in three EDs of Québec, Canada. This first phase of the SuboxED project required selecting clinical experts, describing the patient population, and creating partnerships with pharmacists and opioid agonist treatment clinics. Results The clinical experts developed tools based on literature reviews and national and international guidelines. They also created educational tools and trained over 328 ED clinical staff. In addition, SuboxED ensured that a supply of take-home naloxone and B/n was available in the three ED sites for the study. Conclusion Implementing the proposed clinical algorithm for THN and prescription of B/N was challenging: drug supply and ED staff’s buy-in were among the most notable difficulties of SuboxED. Planning training sessions at three different institutions, each with its own governance structure and clinical culture, local realities and harm reduction priorities was complicated. Engaging already overworked ED teams consistently working in a gridlocked environments, revealed in itself to be a difficult endeavour. In the next phase of SuboxED, we will focus on data collection and analysis to evaluate both the implementation of the protocol through a retrospective review of electronic health records and satisfaction surveys of patients and healthcare professionals.
Monitoring Physical Activity and Sleep Quality in Children with Blindness and Deafness: A Cross-sectional Study
Authors: Jalal Reema M Al , Ibrahim Alaa I. , Abualait Turki S. 
Published: May 2020

Abstract
Background

Child development has critical links to his ability to see and hear. When these senses are impaired, everything is impacted. The aim of this study was to investigate the effect of visual and hearing impairments on physical activity and sleep quality in a comparison with the normal sighted-hearing children.
Methods

This cross-sectional study included 60 Saudi children (20 blind, 20 sensorineural deaf, and 20 sighted-hearing) aged 10 to 15 years old. The physical activity levels and sleep quality were assessed using a waist-mounted ActiGraph accelerometer.
Results

Children with sensorineural deafness were significantly more active (in step rate and activity rate) than the sighted-hearing group (p = 0.05 and 0.003, respectively). Sighted-hearing children and children with blindness had significantly more sedentary time than children with sensorineural deafness (p = 0.004 and 0.03, respectively). Sleep efficiency was significantly higher in children with sensorineural deafness than sighted-hearing children (p = 0.02). No significant difference was recorded in any of the sleep parameters when children with blindness were compared to the sighted-hearing or children with sensorineural deafness (p > 0.05).
Conclusions

The ActiGraph results revealed that children with sensorineural deafness were significantly more active and with higher sleep efficiency than sighted-hearing children. Children with blindness spent more time in sedentary activities and less time in moderate activities than children with sensorineural deafness. There was no significant difference in sleep efficiency when comparing sensorineural deaf and blind children.
Clinical Characteristics and Predictors of the Recurrence of Organising Pneumonia Associated with Rheumatoid Arthritis
Authors: Kamei Ryosuke , YAMASHITA HIROYUKI , Takahashi Yuko , Kaneko Hiroshi 
Published: May 2020

Abstract
Background: Organizing pneumonia (OP) accounts for ~10% of the interstitial lung diseases in patients diagnosed with rheumatoid arthritis (RA). There are only a few published studies that have described RA-associated OP (RA–OP), each with comparatively few study participants. Furthermore, despite the high frequency of secondary flareups, no studies to date have identified factors that predict RA–OP recurrence. This study aimed to clarify the clinical characteristics of RA–OP, to determine the relationship between OP and RA exacerbations, and to identify predictors of RA–OP disease recurrence.Methods: The data of 33 patients with RA–OP admitted to our hospital between 2006 and 2016 were retrospectively analysed.Results: RA onset preceded OP in 82% of patients; OP preceded or co-occurred with RA in 9% each. The median [Q1, Q3] age at first OP onset was 64.0 [55.0, 68.0] years and 5.5 [1.0, 19.3] years after RA onset. At OP onset, 42% of events exhibited unilateral shadows and 76% had normal KL-6; RA disease control remained good in 52% and was exacerbate d in only 18%. Ten (30%) patients experienced OP recurrence with an interval of 13.0 [7.5, 22.5] months between events, with a rate of first recurrence of 127 per 1,000 person-years. Compared with 14 non-recurrent cases, 10 recurrent cases showed lower age at first OP (59.5 years vs. 67.1 years; p = 0.043) and shorter period from RA to first OP (6.4 vs. 14.2 years; p = 0.047) and included more OP-preceding patients (30% vs. 0%; p = 0.029) and more ever-smokers (80% vs. 36%; p = 0.032). OP-preceding patients showed shorter median recurrence-free survival time (15 vs. 136 months; p = 0.009) and higher recurrence risk (hazard ratio, 5.5; p = 0.021). Conclusions: RA–OP showed a high recurrence rate but no association with RA exacerbation. Four predictors of RA–OP recurrence were identified.