bluuebunny/crossref_metadata_2025_split huggingface dataset:
MicroRNA-16-5p Aggravates Myocardial Infarction Injury by Targeting the Expression of Insulin Receptor Substrates 1 and Mediating Myocardial Apoptosis and Angiogenesis
Authors: Wang Xiancan , Shang Yuqiang , Dai Shilin , Wu Wei , Yi Fan , Cheng Long 
Published: December 2019

Purpose:
Myocardial infarction is a common cardiovascular disease. MicroRNA-16-5p
(miR-16-5p) was upregulated in heart and kidney hypoxia/reoxygenation (H/R) injury. However,
the role of miR-16-5p in myocardial infarction injury is still unclear.

Methods:
Human adult ventricular cardiomyocytes (AC16) were treated with ischemia/reperfusion
(H/R). The miR-16-5p level was evaluated through real-time PCR. The activity of lactate dehydrogenase
(LDH) and creatine kinase-MB (CK-MB) was detected via LDH and CK-MB monitoring
kits. Cell viability was examined with 3-(4,5-dimethylthiazol-2-yl)-2,5diphenyltetra-zolium
bromide (MTT) assay. Western blotting was used to analyze the protein levels. The luci-ferase report
assay confirmed the relative luciferase activity.

Results:
miR-16-5p was elevated in H/R-treated AC16 cells. miR-16-5p overexpression and
knockdown were carried out. miR-16-5p knockdown repressed cell apoptosis, attenuated LDH and
CK-MB activities, and enhanced cell viability in H/R-treated AC16 cells. Moreover, miR-16-5p
knockdown promoted angiogenesis in human microvascular endothelial cells (HMVEC), causing
elevation of vascular endothelial growth factor (VEGF), insulin receptor substrates 1 (IRS1),
minichromosome maintenance complex component 2 (MCM2) and proliferating cell nuclear antigen
(PCNA) protein levels. Moreover, miR-16-5p was testified to target IRS1. IRS1 silencing
alleviated miR-16-5p knockdown-mediated inhibition of apoptosis in AC16 cells.

Conclusion:
miR-16-5p knockdown increased cell viability and angiogenesis, as well as inhibited
cell apoptosis by increasing IRS1. These findings indicated that miR-16-5p knockdown may be a
new therapeutic target for myocardial infarction.
Bridging Intravenous Thrombolysis Before Mechanical Thrombectomy for Large Artery Occlusion May be Detrimental with Thrombus Fragmentation
Authors: Yi Ho Jun , Sung Jae Hoon , Lee Dong Hoon 
Published: December 2019

Objective:
We investigated whether intravenous thrombolysis (IVT) affected the outcomes
and complications of mechanical thrombectomy (MT), specifically focusing on thrombus
fragmentation.

Methods:
The patients who underwent MT for large artery occlusion (LAO) were classified into
two groups: MT with prior IVT (MT+IVT) group and MT without prior IVT (MT-IVT) group.
The clinical outcome, successful recanalization with other radiological outcomes, and complications
were compared, between two groups. Subgroup analysis was also performed for patients with
simultaneous application of stent retriever and aspiration.

Results:
There were no significant differences in clinical outcome and successful recanalization
rate, between both groups. However, the ratio of pre- to peri-procedural thrombus fragmentation
was significantly higher in the MT+IVT group (14.6% and 16.2%, respectively; P=0.004) compared
to the MT-IVT group (5.1% and 6.8%, respectively; P=0.008). The MT+IVT group required
more second stent retriever (16.2%), more stent passages (median value = 2), and more occurrence
of distal emboli (3.9%) than the MT-IVT group (7.9%, median value = 1, and 8.1%, respectively)
(P=0.004, 0.008 and 0.018, respectively). In subgroup analysis, the results were similar to those of
the entire patients.

Conclusion:
Thrombus fragmentation of IVT with t-PA before MT resulted in an increased need
for additional rescue therapies, and it could induce more distal emboli. The use of IVT prior to MT
does not affect the clinical outcome and successful recanalization, compared with MT without
prior IVT. Therefore, we need to reconsider the need for IVT before MT.
MicroRNA-451 Aggravates Kainic Acid-induced Seizure and Neuronal Apoptosis by Targeting GDNF
Authors: Weng Ning , Sun Jingbo , Kuang Shixiang , Lan Hai , He Qiansong , Yang Hui , Zhang Lin , Xue Hong 
Published: December 2019

Aim:
Epilepsy is a common and serious neurological disease that causes recurrent episodes,
but its molecular mechanism remains unclear. Abnormal miRNA expression is associated
with epilepsy, including miR-451. This research investigated the role of miR-451 in seizure and its
detailed mechanism.

Methods:
The seizure mice model was induced by kainic acid (KA) injection to the right lateral
cerebral ventricle. Behavioral changes in mice were observed and evaluated by the Racine Scale.
The miR-451 knockout mice were established by adenovirus infection. The in vitro model was
performed by miR-451 mimics transfected HEK-293 cells. The amount of neuronal death and
morphological changes were evaluated by Nissl staining and H&E staining.

Results:
The results showed that miR-451 is up regulated in KA-induced seizure models and miR-
451 knockout decreased the behavior score and improved the pathological changes of the hippocampus.
Besides, MiR-451 knockout inhibited the apoptosis of hippocampal neurons. Bioinformatics
studies have shown that glial cell line-derived neurotrophic factor (GDNF) is a target gene
of miR-451. MiR-451 could negatively regulate the expression of GDNF. GDNF overexpression
could reverse the effect of miR-451 on KA induced brain injury and neuronal apoptosis.

Conclusion:
This research demonstrates that miR-451 can affect the behavior of KA-induced epilepsy
mice and hippocampal neuronal damage by regulating GDNF expression. The results would
provide an experimental foundation for further research about the potential contribution of mi-
RNAs to epilepsy pathophysiology.
Differences in Post-ischemic Motor Recovery and Angiogenesis of MCAO Rats Following Electroacupuncture at Different Acupoints
Authors: Liu Li , Zhang Qun , Xie Hong-Yu , Gua Wei-Jia , Bao Chun-Rong , Wang Nian-Hong , Wu Yi 
Published: December 2019

Background:
Electroacupuncture (EA) can promote nerve and vascular regeneration,
confer neuroprotection, inhibit apoptosis and inflammatory reactions, reduce oxidative stress
injury, regulate neurochemicals and inhibit the formation of brain oedema in cerebral ischemic.
However, the precise site of EA stimulation in the treatment of cerebral ischemic is unclear.

Objective:
In the present study, we investigated the effect of EA at the acupoints of different
meridians in motor function recovery and the involvement of Vascular Endothelial Growth Factor
(VEGF), phosphorylated Protein Kinase B (P-Akt), phosphorylated endothelial nitric oxide
synthase (p-eNOS) and Platelet Endothelial Cell Adhesion Molecule-1(CD31) were examined in
the peri-infarction cortex of rats.

Methods:
The Middle cerebral artery occlusion (MCAO) model or sham surgery was performed in
a total of Ninety male Sprague-Dawley rats. Rats were randomly divided into five groups: a sham
group, a middle cerebral artery occlusion (MCAO) group, a Yang meridian group, a Yin meridian
group and a combined Yang and Yin meridian group. EA stimulus was given during the middle
cerebral artery occlusion. The neurobehavioural function was measured using Modified Neurological
Severity Scores (mNSS), the rotarod test and the ladder rung walking test, and the protein
expression of VEGF, P-Akt, p-eNOS in the peri-infarction cortex was detected by Western blot.
Immunofluorescence was used to measure the vascular density of the peri-infarction cortex.

Results:
EA at different meridian acupoints has no effect on the infarction volume, while EA at
Yin meridian acupoints significantly promoted neurobehavioural functional recovery, increased
the vascular density and enhanced protein kinase B/Endothelial nitric oxide synthase (Akt/eNOS)
phosphorylation and VEGF expression.

Conclusion:
In the early stage of stroke, EA at Yin meridian acupoints can improve neurobehavioural
functional recovery and the mechanism of this effect may be related to the enhanced expression
of VEGF, P-Akt and p-eNOS in the peri-infarction cortex of rats.
Healthcare Workers’ Knowledge of HIV-Exposed Infant Feeding Options and Infant Feeding Counseling Practice in Northern Nigeria
Authors: Iliyasu Zubairu , Galadanci Hadiza S. , Hassan-Hanga Fatima , Abdulrahman Zainab , Tsiga Fatima Ismail , Marryshow Salisha E. , Aliyu Muktar H. 
Published: December 2019

Background:Despite the existence of evidence-based HIV-exposed infant feeding guidelines, infants in Africa still acquire HIV through inappropriate feeding practices.Objective:To identify predictors of HIV-exposed infant feeding knowledge and counseling practice among health care workers (HCW) in Nigeria.Methods:Structured, pretested questionnaires were administered to HCW (n=262) in a tertiary health facility in Kano, Nigeria. Multivariate logistic regression was used to determine predictors of HIV-exposed infant feeding knowledge and counseling practice.Results:Of 262 respondents, (58.0%, n=152) had good knowledge of recommended feeding options. Respondents listed exclusive breastfeeding (57.6%, n=151), human milk substitutes (45.4%, n=119), HIV-negative wet-nursing (37.0%, n=97), heated expressed human milk (20.6%, n=54) and mixed feeding (13.4%, n=35) as appropriate feeding choices. Over half (57.3%, n=150) of the respondents have ever counseled a HIV-positive mother on infant feeding. Knowledge was predicted by female sex (Adjusted Odds Ratio (AOR)=2.47, 95% Confidence Interval (CI):1.35-4.52), profession (physician vs. laboratory scientist, AOR=4.00, 95%CI:1.25-12.87; nurse/midwife vs. laboratory scientist, AOR=2.75, 95%CI:1.17-9.28), infant feeding counseling training (AOR=3.27, 95%CI:1.87-5.71), and number of children (2-4 vs. 0, AOR=1.75, 95%CI:1.23-3.92). Infant feeding counseling was predicted by female sex (AOR=2.85, 95%CI:1.39-5.85), age (>40 vs. <30 years, AOR=3.87, 95%CI:1.27-15.65), knowledge of infant feeding options (good vs. fair/poor, AOR=3.96, 95%CI:2.07-7.59), training (AOR=2.60, 95%CI:1.42-5.32), and profession (physician vs. laboratory scientist, AOR=10.7, 95%CI:2.85-40.54; nurse/midwife vs. laboratory scientist, AOR=4.8, 95%CI:1.26-18.02).Conclusion:The practice of infant feeding counseling among HCW in Nigeria is associated with sex, knowledge, and profession. Our findings may inform the development of targeted training programs for HCW in similar settings.
Tetraamminecopper(II) Sulfate Monohydrate in Oxidative Azide-olefin Cyclo-addition and Three-component Click Reaction
Authors: Sultana Jasmin , Sarma Diganta 
Published: December 2019

Introduction:
An effective Cu-complex, [Cu(NH3)4SO4 • H2O] was prepared conveniently from the
inexpensive and easily available starting reagents in a simple route.

Materials and Methods:
Excellent reactivity of the catalyst was observed towards two competent clickcycloadditions:
(a) oxidative cycloaddition of azides with electron-poor olefins and (b) one-pot cycloaddition
of alkynes with boronic acid and sodium azide under “click-appropriate” conditions.

Results:
No external oxidant, short reaction time, high product yield, wide substrate scope, and aqueous
solvent media make the azide-olefin cycloaddition approach a greener route in contrast to the reported
methods.

Conclusion:
The newly developed mild, green, and rapid three-component strategy shows product diversity
with superb yields at room temperature by reducing the synthetic process time and using only 1 mol % of the
synthesized copper complex.
The Effectiveness of an Educational Intervention Based on Theories and Models on Diabetes Outcomes: A Systematic Review
Authors: Tavakoly Sany Seyedeh Belin , Ferns Gordon A , Jafari Alireza 
Published: December 2019

Background & Introduction:
Patient’s self-management behaviors are essential to control
diabetes. Studies have demonstrated that health educational interventions can improve some aspects of
glycemic control and clinical outcomes, however, it is unclear which education theories underlying
these interventions improve effectiveness. In this review of the literature, we aimed to assess the efficacy
of health education and promotion theories, or models, to improve self-care and self- management
behaviors among patients with type 2 diabetes (T2D).

Methods & Results:
Eight scientific databases (Web of Science, PubMed, Scopus, Magiran, Google
Scholar, Medlib, ProQuest and Scientific Information Database) were searched to identify a published
academic article from 2010 to 2019. We included quasi-experimental, clinical trial and randomized
clinical trial studies. A total of 26 studies including data from 3879 patients with T2D met the inclusion
criteria. We found that the PRECEDE (7/26, 26.92%) and Health Belief Models (7/26, 26.92%) were
the most common models used to assess the efficacy of health education and health promotion models.

Conclusion:
Overall, health promotion and education theories and models have been used as a useful
instrument for improving the self-care behaviors and self-management behaviors among patients with
T2D.
Thyroid Dysfunction in Pregnant Women with Gestational Diabetes Mellitus
Authors: Safian Shahin , Esna-Ashari Farzaneh , Borzouei Shiva 
Published: December 2019

Aims:
Investigation thyroid dysfunction and autoimmunity in pregnant women with gestational
diabetes mellitus.

Background:
This article was written to evaluate the thyroid function and anti-thyroid peroxidase (anti-
TPO) antibodies in pregnant women with gestational diabetes mellitus (GDM).

Method:
A total of 252 women with GDM and 252 healthy pregnant women were enrolled. Thyroid
tests, including TSH, FreeT3, Free T4, and anti-TPO were performed for all women at 24–28 weeks of
gestation. Data analysis was then carried out using SPSS ver. 22.

Result:
There was a significant difference between the experimental group (38.4%) and the control
group (14.06%) in terms of the prevalence of subclinical hypothyroidism (p= 0.016). The frequency of
anti-TPO was higher in the experimental group than the control group and positive anti-TPO was observed
in 18.6% of women with GDM and 10.3% of healthy pregnant women (P= 0.008).

Conclusion:
Thyroid disorders are observed in pregnant women with GDM more frequently than
healthy individuals and it may be thus reasonable to perform thyroid tests routinely.
Field-dependent Morin Transition and Temperature-Dependent Spin-flop in Synthetic Hematite Nanoparticles
Authors: Obaidat Ihab M. , Alaabed Sulaiman , Al-Omari Imad A. , Narayanaswamy Venkatesha , Issa Bashar , Khaleel Abbas 
Published: December 2019

Background:
In nano-size α-Fe2O3 particles, the Morin transition temperature was reported
to be suppressed. This suppression of the TM in nano-size α-Fe2O3 was suggested to be due to high
internal strain and to the enhanced role of surface spins because of the enhanced surface to volume
ratio. It was reported that for nanoparticles of diameters less than 20 nm, no Morin transition was
observed and the antiferromagnetic phase disappears. In addition, annealing of samples was reported
to result in both an increase of TM and a sharper transition which were attributed to the reduction in
defects, crystal growth, or both.

Objective:
In this work, we investigated the role of applied magnetic field in TM, the extent of the
Morin transition, thermal hysteresis, and the spin-flop field in synthetic α-Fe2O3 nanoparticles of
diameter around 20 nm.

Methods:
Hematite nanoparticles were synthesized using the sol-gel method. Morphology and structural
studies of the particles were done using TEM, and XRD, respectively. The XRD patterns confirm
that the particles are hematite with a very small maghemite phase. The average size of the nanoparticles
is estimated from both TEM images and XRD patterns to be around 20 nm. The magnetization
versus temperature measurements were conducted upon heating from 5 K to 400 K and cooling
down back to 5 K at several applied fields between 50 Oe and 500 Oe. Magnetization versus magnetic
field measurements between -5 T and +5 T were conducted at several temperatures in the temperature
range of 2-300 K.

Results:
We report three significant findings in these hematite nanoparticles. Firstly, we report the
occurrence of Morin transition in hematite nanoparticles of such size. Secondly, we report the slight
field dependence of Morin transition temperature. Thirdly, we report the strong temperature dependence
of the spin-flop. Zero-field-cooled magnetization versus temperature measurements were conducted
at several applied magnetic fields.

Conclusion:
From the magnetization versus temperature curves, Morin transition was observed to
occur in all applied fields at Morin transition temperature, TM which is around 250 K with slight field
dependence. From the magnetization versus magnetic field curves, spin-flop in the antiferromagnetic
state was observed and found to be strongly temperature dependent. The results are discussed in
terms of three components of the magnetic phase in our sample. These are the paramagnetic, soft
ferromagnetic, and hard ferromagnetic components.
The Interfacial Effect on H2 Production from Oxidative Steam Reforming of Ethanol Over Rh/Ce1-xLaxO2-δ Nanocatalysts
Authors: Yang Kang , Wang Yafei , Yang Yujie , Hao Hongrui , Han Xue 
Published: December 2019

Background:
The production of hydrogen from catalytic reforming ethanol has attracted
wide attention, which provides a promising way to replace fossil fuels with sustainable energy carriers.

Methods:
In this work, the Ce1-xLaxO2-δ solid solution (CL) supported Rh catalysts (nRh/CL, n = 0.5,
1 and 2 wt.%) were prepared by a traditional impregnation method with a variation of Rh loading.
The different interface structure of nRh/CL catalysts and their catalytic performance in oxidative
steam reforming (OSR) reaction were investigated.

Results:
Rh was loaded by the traditional impregnation method, and ethanol conversion and H2 yield
declined in the order of 1%Rh/CL > 2%Rh/CL > 0.5%Rh/CL.

Conclusion:
The supports of the nRh/CL catalysts were confirmed to be Ce1-xLaxO2-δ solid solution,
but only for the 1%Rh/CL catalyst, the Rh species were well-dispersed on the support and formed a
Rh2O3//Ce1-xLaxO2-δ interface structure. The super-cell structure of Rh3+-O-RE3/4+ (RE = Ce, La) on
the surface of 0.5%Rh/CL catalyst and the formation of interfacial Ce1-x-yLaxRhyO2-δ solid solution
for 2%Rh/CL catalyst had effects on the self-activation of the nRh/CL catalysts. The typical lattice
expansion of Ce1-xLaxO2-δ solid solution lowered the energy for migration. And the excellent hydrogen
and oxygen mobility at the Rh//Ce1-xLaxO2-δ interface for 1%Rh/CL catalyst guaranteed the
good catalytic performance for OSR at low temperature.
Investigation of Formaldehyde Adsorption on Carbon Nanotubes by Density Functional Theory
Authors: Chen Dazhi , Cao Zhongqing , Yuan Yong J. 
Published: December 2019

Background:
Formaldehyde (HCOH) is the most abundant airborne carbonyl indoor volatile
organic compound (VOC), which is well-known to cause serious health effects such as respiratory
system disease, immune system disorders, and central nervous system damage.

Methods:
The interaction between HCOH and intrinsic, congeners of Au, Ag, Cu-doped SWCNTs
were investigated by density functional theory (DFT) to evaluate the detection of formaldehyde.

Results:
The results demonstrated that the less adsorption on the surface of intrinsic SWCNT, an
HCOH molecule tended to be chemisorbed to the Au, Ag, and Cu atoms of doped SWCNT with
larger binding energy of 0.4-0.8 eV and smaller binding distance of 1.9-2.3 Å. Furthermore, charge
transfer and density of state studies indicated tha t the electronic properties changed evidently in the
most stable HCOH-doped SWCNT systems, mainly at the region of -5.5 to -4.5 eV and Fermi level.

Conclusion:
More importantly, the adsorption of HCOH affected the electronic conductance of
doped SWCNT. It is expected that the results obtained in this study could provide a useful theoretical
guidance for the investigation of molecular films interface bonding and design of HCOH sensing
devices.
Targeting Trypanothione Reductase of Leishmanial major to Fight Against Cutaneous Leishmaniasis
Authors: Dukhyil Abdul Aziz A. Bin 
Published: December 2019

Background:1.2-2.0 million cases of leishmaniasis occur annually throughout the world. The available drugs like Amphotericin B, antimonials and miltefosine are unable to fulfill the need due to less effectiveness, high toxicity, resistance, high cost and complex route of administration. Leishmania survives inside the macrophages through different evasion mechanisms; one of that is activation of its trypanothione reductase enzyme which neutralizes the reactive oxygen species generated inside the macrophages to kill the parasites. This enzyme is unique and absent in human, therefore in this study I targeted it for screening of new inhibitors to fight against leishmaniasis.Methods:Homology modeling of Leishmania major trypanothione reductase was performed using Phyre2 server. The homology based modelled protein was validated with PROCHECK analysis. Ligplot analysis was performed to predict the active residues inside the binding pocket. Further, virtual screening of ligand library containing 113 ligands from PubChem Bioassay was performed against the target using AutoDock Vina Tool.Results:Top five ligands showed best binding affinity. The molecule having PubChem CID: 10553746 showed highest binding affinity of -11.3 kcal/mol. Over all this molecule showed highest binding affinity and moderate number of hydrogen bonds. Hopefully, this molecule will be able to block the activity of target enzyme, trypanothione reductase of Leishmania major effectively and may work as new molecules to fight against cutaneous leishmanaisis.Conclusion:This study will help the researchers to identify the new molecules which can block the activity of leishmanial-trypanothione reductase, a novel enzyme of trypanosomatids. These screened inhibitors may also be effective not only in leishmaniasis but also other trypanosomatid-mediated infectious diseases.
UPAYA PENINGKATAN KEAKTIFAN SISWA MELALUI PEMBELAJARAN BERDASARKAN GAYA BELAJAR DI SMK NEGERI 1 SAPTOSARI
Authors: Wibowo Nugroho 
Published: December 2019

Penelitian ini bertujuan untuk mengetahui peningkatan keaktifan siswa melalui penerapan gaya belajar pada mata pelajaran memelihara baterai di SMK Negeri 1 Saptosari. Penelitian ini merupakan penelitian tindakan kelas, dilaksanakan di kelas X TKR B pada semester genap tahun pelajaran 2014/2015. Jenis tindakan penelitian tindakan kelas ini menggunakan model Kemmis dan Taggart. Data dikumpulkan melalui observasi, angket dan dokumentasi, alat yang digunakan untuk mengumpulkan data penelitian: lembar observasi, angket keaktifan siswa, dan checklist dokumentasi. Data yang diperoleh pada penelitian ini dianalisis menggunakan teknik analisis deskriptif dengan presentase, selain itu juga didasarkan pada refleksi tiap siklus tindakan.Hasil penelitian: 1) Pemanfaatan gaya belajar untuk pembelajaran dilakukan melalui tiga tahapan: pencarian data gaya belajar siswa, pengelompokan siswa, dan pemberian materi sesuai gaya belajar; 2) Pemanfaatan gaya belajar dapat meningkatkan keaktifan siswa berdasarkan lima indikator yaitu: perhatian, kerjasama dan hubungan sosial, mengemukakan pendapat atau ide, pemecahan masalah, dan disiplin.
Chrzest jako duchowe małżeństwo w nauczaniu Jana Chryzostoma
Authors: Grzywa Anna 
Published: December 2019

Jan Chryzostom w swoim bogatym dorobku duszpasterskim pozostawił po sobie także katechezy skierowane do katechumenów i nowo ochrzczonych. W dostępnych pouczeniach katechetycznych możemy dostrzec, iż szczególnie często odwołuje się on do wizji chrztu jako duchowego małżeństwa. W takim rozumieniu Chrystus – doskonały oblubieniec – przychodzi poślubić oblubienicę – katechumena – a jego inicjatywa i bezwarunkowa miłość przemienia grzesznego i pełnego słabości kandydata, czego symbolem przywoływanym przez katechetę staje się także lśniąca szata weselna. Artykuł wskazuje konteksty, w których Jan Chryzostom przywołuje wskazane porównanie oraz próbuje odpowiedzieć na pytanie, skąd u autora tak liczne odwołania do obrazu małżeństwa w odniesieniu do sakramentu chrztu.