bluuebunny/crossref_metadata_2025_split huggingface dataset:
Serratia liquefaciens FG3 isolated from a metallophyte plant sheds light on the evolution and mechanisms of adaptive traits in extreme environments
Authors: Caneschi Washington Luiz , Sanchez Angélica Bianchini , Felestrino Érica Barbosa , Lemes Camila Gracyelle de Carvalho , Cordeiro Isabella Ferreira , Fonseca Natasha Peixoto , Villa Morghana Marina , Vieira Izadora Tabuso , Moraes Lauro Ângelo Gonçalves , Assis Renata de Almeida Barbosa , do Carmo Flávio Fonseca , Kamino Luciana Hiromi Yoshino , Silva Robson Soares , Ferro Jesus Aparecido , Ferro Maria Inês Tiraboschi , Ferreira Rafael Marini , Santos Vera Lúcia , Silva Ubiana de Cássia Mourão , Almeida Nalvo Franco , Varani Alessandro de Mello , Garcia Camila Carrião Machado , Setubal João Carlos , Moreira Leandro Marcio 
Published: November 2019

AbstractSerratia liquefaciens strain FG3 (SlFG3), isolated from the flower of Stachytarpheta glabra in the Brazilian ferruginous fields, has distinctive genomic, adaptive, and biotechnological potential. Herein, using a combination of genomics and molecular approaches, we unlocked the evolution of the adaptive traits acquired by S1FG3, which exhibits the second largest chromosome containing the largest conjugative plasmids described for Serratia. Comparative analysis revealed the presence of 18 genomic islands and 311 unique protein families involved in distinct adaptive features. S1FG3 has a diversified repertoire of genes associated with Nonribosomal peptides (NRPs/PKS), a complete and functional cluster related to cellulose synthesis, and an extensive and functional repertoire of oxidative metabolism genes. In addition, S1FG3 possesses a complete pathway related to protocatecuate and chloroaromatic degradation, and a complete repertoire of genes related to DNA repair and protection that includes mechanisms related to UV light tolerance, redox process resistance, and a laterally acquired capacity to protect DNA using phosphorothioation. These findings summarize that SlFG3 is well-adapted to different biotic and abiotic stress situations imposed by extreme conditions associated with ferruginous fields, unlocking the impact of the lateral gene transfer to adjust the genome for extreme environments, and providing insight into the evolution of prokaryotes.
Prostaglandin E1 protects cardiomyocytes against hypoxia-reperfusion induced injury via the miR-21-5p/FASLG axis
Authors: Tang Mingxiang , Pan Hongwei , Zheng Zhaofen , Guo Yin , Peng Jianqiang , Yang Jun , Luo Yangping , He Jin , Yan Sulan , Wang Peng , Zhang Yi , Zhou Yulu 
Published: November 2019

Abstract
Background: Prostaglandin-E1 (PGE1) is a potent vasodilator with anti-inflammatory and antiplatelet effects. However, the mechanism by which PGE1 contributes to the amelioration of cardiac injury remains unclear.
Methods: The present study was designed to investigate how PGE1 protects against hypoxia/reoxygenation (H/R)-induced injuries by regulating microRNA-21-5p (miR-21-5p) and fas ligand (FASLG). Rat H9C2 cells and isolated primary cardiomyocytes were cultured under hypoxic conditions for 6 h (6H, hypoxia for 6 h), and reoxygenated for periods of 6 (6R, reoxygenation for 6 h), 12, and 24 h, respectively. Cells from the 6H/6R group were treated with various doses of PGE1; after which, their levels of viability and apoptosis were detected.
Results: The 6H/6R treatment regimen induced the maximum level of H9C2 cell apoptosis, which was accompanied by the highest levels of Bcl-2-associated X protein (Bax) and cleaved-caspase-3 expression and the lowest level of B-cell lymphoma 2 (Bcl-2) expression. Treatment with PGE1 significantly diminished the cell cytotoxicity and apoptosis induced by the 6H/6R regimen, and also decreased expression of IL-2, IL-6, P-p65, TNF-α, and cleaved-caspase-3. In addition, we proved that PGE1 up-regulated miR-21-5p expression in rat cardiomyocytes exposed to conditions that produce H/R injury. FASLG was a direct target of miR-21-5p, and PGE1 reduced the ability of H/R-injured rat cardiomyocytes to undergo apoptosis by affecting the miR-21-5p/FASLG axis. In addition, we proved that PGE1 could protect primary cardiomyocytes against H/R-induced injuries.
Conclusions: These results indicate that PGE1 exerts cardioprotective effects in H9C2 cells during H/R by regulating the miR-21-5p/FASLG axis.
Interpretation and Validation of Maximum Absorbance Data Obtained from Turbidimetry Analysis of Plasma Clots
Authors: Pieters Marlien , Guthold Martin , Nunes Claudia M. , de Lange Zelda 
Published: November 2019

AbstractTurbidimetry is used to characterize fibrin clot properties. In purified systems, maximum absorbance (MA) directly relates to fibrin fiber cross-sectional area. However, in plasma samples there are discrepancies in the relationships between MA and fibrinogen concentration, fiber diameter, other clot properties, and cardiovascular disease outcomes, which complicate data interpretation. This study aims to advance understanding of MA of plasma clots through testing how well it relates to fundamental dependence on fibrinogen concentration and fiber diameter as predicted by light scattering theory, other clot properties and lifestyle, and biochemical variables. Plasma samples from 30 apparently healthy individuals with a fibrinogen concentration from 2.4 to 6.4 g/L were included. We performed turbidimetry, permeability, scanning electron microscopy, and rheometry on in vitro formed plasma clots. MA correlated more strongly with fibrinogen concentration (r = 0.65; p < 0.001) than with fiber diameter (r = 0.47; p = 0.01), which combined explained only 46% of the MA variance. Of additional variables measured, only low-density lipoprotein cholesterol correlated with MA (r = 0.46; p = 0.01) and clot lysis (r = 0.62; p < 0.0001) but not with fiber diameter or fibrinogen concentration. MA correlated with clot lysis time (r = 0.59; p = 0.001), storage modulus (r = 0.61; p = 0.001), and loss modulus (r = 0.59; p = 0.001), and negatively with clot permeability (r = –0.60; p = 0.001) also after adjustment for fibrinogen concentration and fiber diameter. Increased MA is indicative of a prothrombotic clot phenotype irrespective of fibrinogen concentration. MA is more indicative of overall clot density than of fiber diameter. Other plasma components can alter internal fiber density without altering fiber diameter and should be considered when interpreting MA of plasma samples.
Secondary Management of Mandible Fractures
Authors: Reddy Likith , Lee Daniel , Vincent Aurora , Shokri Tom , Sokoya Mofiyinfolu , Ducic Yadranko 
Published: November 2019

AbstractMandibular fractures are the most common facial fractures that need surgical intervention. If untreated, these fractures affect a patient's occlusion, degree of mouth opening, and facial symmetry, and could cause infection with significant pain. The goal of any surgical intervention is to restore the preinjury occlusion, even if the preinjury occlusion is abnormal. Initial therapies, whether surgical or conservative, are not always successful, however, and revision or delayed surgical intervention can be challenging. Herein, we review common causes of failure of primary surgical management of mandibular fractures and provide tips to successful secondary intervention.
Radiographic Native Femoral Head Size Correlates with Acetabular Cup Size in Primary Total Hip Arthroplasty
Authors: Pfeufer Daniel , Gililland Jeremy , Ajinkya Rane , Duensing Ian , Anderson Mike B. , Peters Christopher L. , Pelt Christopher E. 
Published: November 2019

AbstractThere is very little literature on whether a simple radiographic measurement of native femoral head size correlates with implanted acetabular cup size. We hypothesized that there would be a high correlation between the radiographically measured native femoral head and implanted acetabular component size in primary total hip arthroplasty (THA). We conducted a retrospective study of 277 consecutive patients who underwent primary THA. Patients with prior contralateral THA or a history of congenital deformity were excluded (n = 95). We used the postoperative anteroposterior (AP) pelvic view to calibrate the image using known implanted femoral head component size. We then measured the diameter of the contralateral native femoral head under the assumption that femoral heads are generally symmetrical in patients. Two of the authors performed all measurements. To determine if native femoral head size was correlated with acetabular component size we used the Pearson correlation coefficient (r). There was a high correlation between contralateral native femoral head size and acetabular component size (r = 0.86, 95% confidence interval [CI]: 0.82–0.89). The median difference in radiographically measured native femoral head size and acetabular component size was 7 mm (interquartile range [IQR] = 5–8). Our data showed a high correlation between acetabular component size and radiographically measured contralateral native femoral head size, with the difference being 7 mm. In addition to verifying the actual femoral head size with calipers during surgery, this simple radiographic measurement, which could be done on the affected or contralateral hip, may help surgeons to plan better and improve preoperative templating in primary THA.
Opioid Requirement following Arthroscopic Knee Surgery: Are There Predictive Factors Associated with Long-Term Use
Authors: Glogovac Georgina , Kennedy Mark , Parman Michael D. , Bowers Katherine A. , Colosimo Angelo J. , Grawe Brian M. 
Published: November 2019

AbstractThe purpose of this study is to identify patterns of postoperative narcotic use and determine the impact of psychosocial and perioperative factors on postoperative opioid consumption following arthroscopic knee surgery. Fifty consecutive patients undergoing arthroscopic knee surgery were prospectively enrolled. Patients were contacted via telephone at 1 week postoperatively to report their pain level and opioid consumption. The patient was contacted again at 2 weeks, 4 weeks, and 90 days as necessary until opioid cessation, at which time the patient's plan for unused pills was inquired. Opioid consumption was compared using t-tests and one-way analysis of variance for demographic and surgical factors. Linear regression was used to determine whether the Pain Catastrophizing Scale (PCS), Resilience Scale (RS-11), International Knee Documentation Committee questionnaire, or patient-reported pain at 1 week predicted higher opioid consumption. The average morphine equivalent dose of opioid consumption was 142 mg. Sixty-four percent consumed less than 100 mg, and 68% discontinued opioid use by 1 week postoperatively. Seventy-four percent reported surplus pills, and 49% of those patients plans for pill disposal. Factors associated with higher consumption included undergoing a major procedure, having a regional anesthesia block, and higher area deprivation index score (p < 0.05). Higher PCS scores and reported average pain level at 1 week were predictive of higher opioid consumption (p < 0.05). In conclusion, a majority of patients undergoing outpatient knee surgery did not require the entirety of their narcotic prescription. The majority of patients consumed less than 100 mg of morphine equivalents and discontinued opioid use by 1 week postoperatively. Ligament reconstruction, living in an area with a higher index of deprivation, and higher score on the PCS were associated with greater opioid consumption. Overall, patient knowledge regarding opioid disposal was poor, and patients would likely benefit from additional education prior to surgery.
2.3 Dual Catalysis with Two Organocatalysts
Authors: Wang H.-Y. , Zhao G. 
Published: November 2019

AbstractThe field of asymmetric organocatalysis has attracted the attention of chemists due to the environmentally benign conditions, particularly for the synthesis of chiral molecules, bioactive compounds, natural products, and drugs. Besides the conventional approaches using a single organocatalyst in asymmetric reactions, dual catalysis with two organocatalysts has emerged as an important strategy for resolving existing challenging problems, including the synthesis of complex molecules, improvement of enantioselectivities, and the development of new catalytic mechanisms. In this review, selected recent examples of the combination of two organocatalysts are covered in detail. Moreover, future perspectives are also described.
Femtosecond laser system based on thin rod Yb : YAG active elements with an output power of 110 W
Authors: Yang J. , Lee B. , Kim J.W. , Jeong B. , Sall E.G. , Chizhov S.A. , Heo D. , Yashin V.E. , Kim G.H. 
Published: November 2019

Abstract
A three-stage laser amplification system based on thin Yb : YAG rods with diode laser pumping is developed and fabricated. In this system, the average power of a continuous train of femtosecond pulses from a Yb : KGW master oscillator with a pulse repetition rate of 80 MHz and initial pulse duration of 128 fs was raised by a factor of 100 to above 100 W. Preliminarily chirped laser pulses provided compensation of dispersion in optical elements of the system and obtaining 580-fs output pulses. The longer duration of output pulses relates to spectrum narrowing in the amplifiers.
Spectral and temporal dynamics of ultrashort pulses in a holmium-doped fibre amplifier
Authors: Filatova S.A. , Kamynin V.A. , Zhluktova I.V. , Trikshev A.I. , Arutyunyan N.R. , Rybin M.G. , Obraztsova E.D. , Batov D.T. , Voropaev V.S. , Tsvetkov V.B. 
Published: November 2019

Abstract
We have optimised a hybrid mode-locked holmium-doped fibre laser and a holmium-doped fibre amplifier for obtaining stable pulses as short as possible. Temporal and spectral characteristics of pulsed light have been measured as functions of pump power. Lasing has been achieved in the wavelength range 2066 – 2068 nm, with a full width at half maximum of the emission spectrum from 3 to 4 nm. The pulse duration does not exceed 1 ps and the pulse energy ranges from 0.2 to 0.5 nJ. To raise the pulse energy, holmium-doped fibre amplifiers based on two types of active fibre have been used. We have demonstrated a decrease in the width of the central part of the pulse autocorrelation function and considerable broadening of the spectrum of the pulses. The maximum average power at the amplifier output exceeds 200 mW.
Design principles for modern fibre-optic communication lines
Authors: Konyshev V.A. , Nanii O.E. , Novikov A.G. , Treshchikov V.N. , Ubaydullaev R.R. 
Published: November 2019

Abstract
We discuss the design issues and characteristics of coherent fibre-optic communication networks taking into account the many physical effects that simultaneously influence signal propagation. Algorithms and easy-to-use ‘engineering’ techniques are proposed that allow network performance to be predicted and the design to be significantly simplified.
The nanometre limits of ballistic and diffusive hot-hole mediated nonlocal molecular manipulation
Authors: Etheridge H G , Rusimova K R , Sloan P A 
Published: November 2019

Abstract
We report an experimental investigation into the surface-specific and experimental limits of the range of STM induced nonlocal molecular manipulation. We measure the spot-size of the nonlocal manipulation of bromobenzene molecules on the Si(111)-7 × 7 surface at room temperature at two voltages and for a wide range of charge-injection times (number of hot charge-carriers) from 1 s up to 500 s. The results conform to an initially ballistic, 6–10 nm, and then hot-hole diffusive, 10–30 nm, transport away from the localised injection site. This work gives further confirmation that nonlocal molecular manipulation by STM directly reveals the ultrafast transport properties of hot-charge carriers at surfaces.
Enhanced optical squeezing from quasi-bound states in the continuum and Fano resonances without nonlinearity
Authors: Li Zhixin , Zhang Xiangdong 
Published: November 2019

Abstract
To achieve a high degree of quantum noise squeezing, nonlinear optical interaction process is often employed. Here, we propose to utilize quasi-bound states in the continuum (quasi-BICs) and Fano resonances to enhance optical squeezing without nonlinearity. The theory of quantization for electromagnetic fields in the periodic nanostructure with dispersion and absorption has been developed by means of the Green’s function technique with the plane wave expansion method. The quasi-BICs and Fano resonances of radiation modes are realized by designing the photonic crystal slab structure. Based on these quasi-BICs and Fano resonances, we demonstrate that strong squeezed states can be realized by using the balanced homodyne detection scheme. The squeezing degree can be improved by more than 14 times when a weak squeezed states passes through the structure with quasi-BICs and Fano resonances. The advantage of this method is that it is not only efficient but also easy to implement because the nonlinear optical processes are not employed, which is very beneficial for the quantum information processing and precision metrology.
An attractiveness-based model for human mobility in all spatial ranges
Authors: Zhou Jianfeng , Fan Zhong-yan , Ng Kai-Tat , Tang Wallace K S 
Published: November 2019

Abstract
In the past decade, various aspects of human mobility, from individual to population levels in both spatial and time scales, have been studied. However, existing human mobility models still fail to describe activities in all spatial ranges, while in particular, local activities like self-loop fluxes are generally ignored. Moreover, the regional attractiveness, a basic but useful concept in mobility model design, remains difficult to be quantified due to its intricacies. To resolve these two fundamental issues, we introduce a trip competition mechanism to empower a mobility model to estimate population fluxes in all spatial ranges. The model includes attraction scores of regions in concern, obtained via optimizing the working population distributions. Its capability of predicting a variety of mobility patterns is verified by empirical data from three different countries, and its accuracy outperforms those of existing models. The quantified attractiveness is also found to be highly correlated with common socioeconomic indicators, and is able to act as a distinct metric to characterize a region.
Research on the Development Roadmap of Wind Power Industry Under High Renewable Energy Penetration
Authors: Li Lingyun , Gan Jingshuang , Guo Xinju 
Published: November 2019

Abstract
Renewable energy with high resource potential and less environmental pollution are receiving more and more attention. Wind power is a relatively mature energy source in renewable energy. As the internal and external uncertainty of the wind power industry chain are various and complex, it is urgent to clarify the uncertainty and relationship. Through big data technique, main uncertainty impacted on the development of the wind power industry chain is identified. And an interpretative structural model is established to analyze the hierarchy structure to the uncertainty. The results can provide valuable reference to identify and solve the key problems in the development roadmap of wind power industry, as well as to make scientific decision.
Experimental Study on Recovery of Cyanide Tailings Slurry by Membrane Separation in a Gold Mine
Authors: Liu Yanli , Liu Fengbiao , Zheng Xi , Yang Xiaosong , Wang Fang 
Published: November 2019

Abstract
In view of the difficulty in recovering valuable metals and cyanides from cyanide tailings pulp in gold mines, the laboratory exploratory test of recovering gold and silver from the tailings pulp produced by a CIP process was carried out by using the technology of “solid-liquid separation + membrane separation and purification + adsorption and recovery of gold and silver by activated carbon”. The results showed that the cyanide tailings slurry was separated by solid-liquid in the laboratory and stabilized by chemicals, and the dry slag was identified as a general solid waste according to the results of the identification standard for hazardous waste leaching toxicity GB5085.3-2007. The screened reverse osmosis membrane test showed that 95% of the permeate water recovery rate were obtained. The concentration of cyanide in membrane concentrate water reached 807.9 mg/L, the concentration of copper, gold and silver reached 0.43 mg/L and 3.91 mg/L respectively after being concentrated from below the detection limit, and the concentration was 19.9, 14.3 and 18.6 times respectively. The adsorption test of membrane concentrate water by activated carbon showed that gold, silver and copper were absorbed in membrane concentrated water, the adsorption rates reached 99.76%, 96.16% and 9.09% respectively, which facilitated the subsequent selective recovery of gold and silver. The recovery of valuable metals and cyanide from cyanide tailings slurry by the process of “solid-liquid separation + membrane separation and purification + adsorption and recovery of gold and silver by activated carbon” had a good effect.
Method for generating high-precision calibration force for low thrust measurement
Authors: Chen Ji , Zhou Weijing , Hong Yanji 
Published: November 2019

Abstract
The micro-thrust measurement system plays an important role in the development of micro-nano satellites. The method of obtaining high-precision calibration force is one of the key technologies of the micro-thrust measurement system. The smaller the calibration force error, the higher the calibration accuracy of the system parameters, and the higher the measurement accuracy of the measurement system. In this paper, a high-precision calibration force generation method is obtained through experiments and experiments using magnets and coils.
Study on the efficacy of anaerobic biological treatment of industrial wastewater
Authors: Chang Min 
Published: November 2019

Abstract
In this research, we examined the internal circulation (IC) reactor performance on fur processing wastewater and explored the optimum operation parameters of IC reactor with high concentration volatile fatty acids (VFA) by a 60d field test in middle temprature. The results showed that the wastewater can be adapted to the IC reactor and start rapidly. In steady operation stage, when the influent CODcr concentration stabilises at under 3000 mg·L-1 and the acidibility is at around 45%, there is no apparent VFA accumulation and the effluent CODcr removal rate remained stable at about 55%. When the concentration of influent CODcr exceeds 3000 mg·L-1 and fluctuations obviously, the average removal rate of CODcr is about 50%. The average removal rate of grease is 61.3%, and the HRT is 6 h. Compared with the existing hydrolysis acidification and aerobic treatment, IC reactor treatment has improved the efficiency of VFA degradation significantly, which indicates that IC reactor has great engineering applicability for treating fur processing wastewater.
Advanced Oxidation Technology for Refractory Organic Compounds in Wastewater
Authors: Shi Runzhe 
Published: November 2019

Abstract
As a relatively important task in the current industrialization development, wastewater treatment has become increasingly demanding in the context of China’s current rapid industrialization. In order to improve the quality of industrial wastewater treatment, it is necessary to clarify the relevant treatment technologies according to the requirements of the treatment process. Meanwhile, scientific treatment methods should also be combined with the requirements in the wastewater treatment technology, especially for the treatment of refractory organic compounds in wastewater. In other words the quality of wastewater treatment can be improved only by scientific treatment of refractory substances in wastewater. Therefore, this paper analyzes the advanced oxidation technology for degrading refractory organic compounds in wastewater, with the aim to clarify the treatment of refractory organics in wastewater and thenimprove wastewater treatment quality.