bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of circular RNA hsa_circ_0044556 and its effect on the progression of colorectal cancer
Authors: Jing Liang , Wu Junhui , Tang Xiaocheng , Ma Min , Long Fei , Tian Buning , Lin Changwei 
Published: June 2020

Abstract
Background: Circular RNAs (circRNAs) are a novel class of noncoding RNAs. Increasing evidence indicates that circRNAs play an important role in the occurrence and development of tumors. However, the role of circRNA hsa_circ_0044556 in the progression of colorectal cancer (CRC) remains unclear. Methods: First, we searched for differentially expressed circRNAs using a circRNA microarray in paired CRC and adjacent normal tissues. The circRNA hsa_circ_0044556 was screened out from the existing CRC circRNA microarray in the Gene Expression Omnibus database and our microarray. The clinical significance of hsa_circ_0044556 expression level in CRC patients was then investigated. Finally, the functions of the targets of this circRNA were determined in CRC cell lines.Results:Hsa_circ_0044556 was highly expressed in CRC patients and was positively correlated with tumor stage and lymph node metastasis. In CRC cell lines, the proliferation, migration, and invasion of cancer cells were inhibited by knocking down hsa_circ_0044556 expression.Conclusion: Hsa_circ_0044556 promoted the progression of CRC. It is possible that hsa_circ_0044556 will become a novel biomarker or therapeutic target for CRC.
MicroRNA-214-3p Inhibits the Stem-Like Properties of Lung Squamous Cell Cancer by Targeting YAP1
Authors: Lu Tingting , Yang Ying , Li Ziming , Lu Shun 
Published: June 2020

Abstract
Background: Emerging evidence reveals that microRNAs (miRNAs) play a crucial role in tumor progression, but the underlying mechanism of microRNAs in lung squamous cell cancer (LSCC) remains unclear. Method : Western-blotting and quantitative real-time PCR (q-PCR) were carried out to detect mRNA and protein expression. Cell proliferation was evaluated by Cell Counting Kit-8 (CCK-8), colony-forming assay or sphere-forming assay, respectively. Results: MiR-214-3p was markedly de-regulated in LSCC tissues and was inversely related to the level of Yes-associated protein1 (YAP1), which is the core transcription regulator of the Hippo signaling pathway. Kaplan-Meier survival curves illustrated that patients with high miR-214-3p expression demonstrated more favorable clinical outcomes. MiR-214-3p overexpression (OE) repressed proliferation and cancer stem-like cells (CSCs) properties in vitro and in vivo xenograft mouse model. Mechanistically, luciferase activity assay revealed that miR-214-3p directly targets YAP1 by specifically binding on the 3’ UTR of YAP1. Conclusion: MiR-214-3p plays a pivotal role in CSCs properties by targeting YAP1, which provides a potential treatment strategy for LSCC patients.
Time series modeling of air pollution and its association with season and climate variables in istanbul, turkey
Authors: al-najjar hazem , Al-Rousan Nadia , Elhaty Ismail A. 
Published: June 2020

Abstract
Air pollution depends on seasons, wind speed, temperature, wind direction and air pressure. The effect of different seasons on air pollution is not fully addressed in the reported works. The current study investigated the impact of season on air pollutants including SO2, PM10, NO, NOX, and O3 using Nonlinear AutoregRessive network with eXogenous inputs (NARX) method. In the applied methodology, a feature selection was used with each pollutant to find the most important season(s). Afterward, six models are designed based on the feature selection to show the impact of seasons in finding the concentration of pollutants. A case study is conducted on Esenyurt which is one of the most populated and industrialized places in Istanbul to validate the proposed framework. The performance of using all of the designed models with different pollutants showed that using season effect led to improving the performance of predictor and generating high R2 and low error functions.
In Vitro Anti Sickling Activity of HyphaeneThebaica Fruits Extract on Sickle Cell
Authors: Fadlalla Toga Abdalazim , Yousif Nusiba Abdullah , Omer Rayan Hamid , Elbasheir Mohamed Mobarak , Elradi WalaEldin Osman , Abdallah Elharam Ibrahim 
Published: June 2020

Abstract
Background: Traditional medicine had been used alongside synthetic pharmaceutical products to enhance health management. Due to the high mortality rate of sickle cell patients, previous studies have been indicated that some medicinal plants have shown an anti-sickling activity, which indicates a new therapeutic way to manage people who are affected by these disorders. The current study aimed to assess in vitro-anti-sickling activity of Hyphaene thebaica (H thebaica) (Doum) fruit. Materials and methods: Blood samples used in the evaluation of the anti-sickling activity of Hyphaene Thebaica Fruits extract in this study was taken from patients known to had Sickle cell disease (HB -SS ) attending the Sickle Cell Clinic in Khartoum state. Emmel test was used to assess anti-sickling activity of this plant. Result: A significant increase in the percentage of unsickled Red blood cells with p-value 0.05 >was observed after incubation of RBCs with 2% sodium metabisulfite in the presence of three different concentration (1000, 500 and 250) μg/ml of Hyphened thebaica for two type of extraction (aqueous and methanol) extract. Conclusion: This study approved that H.thebaica (Doum ) fruit extract had a strong anti- sickling activity ; it could be used for management of sickle cell disease.
Decreased Apolipoprotein A-I Level Indicates Poor Prognosis in Hodgkin Lymphoma
Authors: Shen Jing , Yang Ronghui , Yang Wei 
Published: June 2020

Abstract
Background Recent studies have reported of an association between Abnormal lipid metabolism and many kind of malignant tumors, and the level of lipid varies with the outcome of the disease. Some data have indicated that the serum level of Apolipoprotein A-I(Apo A-I) in serum was down-regulated in some tumors, but there is few report about it in hodgkin lymphoma. In this study, we aimed to determine the change and clinical significance of serum level Apolipoprotein A-I in hodgkin lymphoma patients. Methods A total of 88 newly diagnosed cases of HL were analyzed retrospectively. Results Our study showed that the serum level of ApoA-I has significant differences in some clinical features of HL, and it dynamically changed with different stages. Divied patients by whether remission or not, we noticed that the non-remission group had lower ApoA-I than remission group. According the result of multivariate analysis decreased ApoA-I reduction become the independent risk factors of HL. On the basis of Kaplan-Meier survival analysis between high ApoA-I subgroup and low ApoA-I subgroup . we found the lower ApoA-I patients had short OS rate and PFS. The serum ApoA-I level was able to differentiate cases with poor outcomes from cases with good outcomes.Conclusions Our results showed that the serum level of ApoA-I was helpful for predicting HL prognosis.
Maternal Childhood Experiences and Rapid Repeat Pregnancy in a Low-Income, Urban Cohort
Authors: Shreffler Karina M. , Joachims Christine N. , Tiemeyer Stacy 
Published: June 2020

Abstract
Background

Rapid repeat pregnancy (RRP; < 18 months between pregnancies) is associated with higher risk of adverse maternal and child health outcomes. Drawing from attachment theory, we sought to examine the association between maternal childhood neglect and RRP as well as identify a protective childhood experience—caregiving—that reduces the impact of childhood neglect for RRP.
Methods

Data for the current study came from a prospective clinic-based cohort study conducted in 2017–2018 in a South-Central U.S. state. The sample includes 111 pregnant women (ages 17–38) who had been pregnant more than once. Poisson regression analysis was used to examine the associations between maternal childhood experiences and number of RRP.
Results

On average, participants reported 1.02 RRP. Approximately 35% of the sample reported experiencing neglect during childhood. Controlling for sociodemographic, psychological, and childhood characteristics, childhood neglect was positively associated with RRP, whereas caregiving responsibilities during childhood was negatively associated with RRP. Interaction results indicate that the association between childhood neglect and RRP only holds for those who did not have caregiving responsibilities during childhood.
Conclusions

Study findings highlight the importance of childhood experiences for RRP. Further research is needed to explore the moderating effect of caregiving and its implications for (1) treatment of childhood neglect and (2) prevention of RRP.
Serum CA19-9 Level is Correlated with Liver Inflammation, Cirrhosis, and Poor Prognosis after Curative Resection in Hepatitis B-related Hepatocellular Carcinoma
Authors: Zhang Wei , Wang Ying-Ying , Yang Bo , Chen Lu , Zhang Ze-Wu , Cao Guang-Tai , Huang Xiao-Yu , Li Guang-Hao , Li Hui-Kai , Cui Yun-Long , Wu Qiang , Zhang Ti , Song Tian-Qiang , Li Qiang 
Published: June 2020

Abstract
Background: The relationship between serum carbohydrate antigen 19-9 (CA19-9) and hepatocellular carcinoma (HCC) is unclear. To explore the prognostic value of preoperative CA19-9 in α-fetoprotein (AFP)-positive and -negative HCC with hepatitis B virus (HBV) background (HBV-HCC).Methods: We evaluated HBV-HCC patients who underwent curative resection (Cohort 1). Recurrence-free survival (RFS) and overall survival (OS) were assessed, and the clinicopathological characteristics were compared according to a CA19-9 cutoff of ≥ 39 U/ml. Immunohistochemical staining of CA19-9 in HCC tumor tissue and background liver were quantified in another cohort of 216 patients with resected HCC (Cohort 2). Immunohistochemical staining or serum CA19-9 level was also compared between patients with intrahepatic cholangiocarcinoma (ICC) (Cohort 3).Results: In Cohort 1, CA19-9 ≥ 39 U/ml was an independent risk factor for RFS (HR=1.507, 95% CI=1.087-2.091, p=0.014) and OS (HR=1.646, 95% CI=1.146-2.366, p=0.007) in both AFP-positive and AFP-negative patients. CA19-9 ≥ 39 U/ml was also associated with significantly higher incidence of macrovascular invasion compared with CA19-9 < 39 U/ml (23.0% vs. 7.2%, p=0.002), elevated aminotransferase and aspartate aminotransferase to platelet ratio index, and lower albumin. In contrast, no association was found between CA19-9 and systemic inflammation. CA19-9 expression was found exclusively in the background liver but not in HCC tumor cells. In contrast, tumor tissue was the main source of CA19-9 in ICC patients.Conclusions: CA19-9 ≥ 39 U/ml was associated with lower OS and RFS in both AFP-positive and negative HCC patients. CA19-9 is secreted by the background liver but not by tumor cells. Thus, CA19-9 is a prognostic marker for liver inflammation and cirrhosis in HCC.
The Clinical Value of Ficolin-3 Gene Polymorphism in Rheumatic Heart Disease. An Egyptian Adolescents Study
Authors: Gomaa Maher H. , Khidr Emad Gamil , Elshafei Ahmed , Hamza Hala S. , Fattouh Aya M. , El-Husseiny Ahmed A. , Aglan Ahmed , Eldeib Mahmoud Gomaa 
Published: June 2020

Abstract
Background: The innate immunity molecules against Streptococcus pyogenes infections include ficolin-3 that was thought to have a role in autoimmune diseases resulting from this infection such as rheumatic fever (RF) which goes on and leads to its most serious sequel, rheumatic heart disease (RHD). We aimed in this study to disclose if there is an association between ficolin-3 gene polymorphisms (rs4494157 and rs10794501) and RF with or without RHD for the first time in Egyptian adolescents. Methods: This study was carried out on 160 RF patients; eighty patients had RHD and eighty didn't have RHD. Besides, eighty ethnically and age-matched healthy subjects were selected as control. Ficolin-3 gene polymorphisms (rs4494157 and rs10794501) were genotyped by TaqMan® allelic discrimination assay. Serum ficolin-3 was quantitatively determined by ELISA. Results: Regarding ficolin-3 gene polymorphisms; no differences in the frequency of rs10794501 were found between the investigated groups, while polymorphism of rs4494157 showed a significant correlation between AA homozygous genotype, high serum ficolin-3, and RHD risk. Conclusion: Elevated serum ficolin-3 and carriage of AA genotype of rs4494157 appeared to be involved in RF and RHD pathogenesis and may be predictive tools for early recognition of RHD prone RF patients, and subsequent early prophylactic interventions.
Longevity of Chinese Chestnut Correlates with Stable Root Microbiomes
Authors: Cheng Xu , Yan Zhichun , Schmitz Lucas , Schneijderberg Martinus , Yan Jundi , Ge Yueyang , Lan Yanping , Wang Yiyang , Li Guangdong , Yang Liu , Qin Ling , Cao Qingqin , Bisseling Ton 
Published: June 2020

Abstract
The authors have requested that this preprint be removed from Research Square.
Radiofrequency Ablation Combined with Stent Placement versus Stent Placement Alone for Malignant Biliary Obstruction: A Systematic Review and Meta-analysis
Authors: Chen Hongyang , Zhu Chunxian , Sun Leimin 
Published: June 2020

Abstract
Background: Malignant biliary obstruction was typically diagnosed at an advanced stage due to painless jaundice. Stent placement is the therapy of choice in this set of patients. Radiofrequency ablation is an ablative therapy which has been well recognized for treating malignant biliary strictures. This meta-analysis aims to help to better understand the safety and efficacy of biliary Radiofrequency ablation combined with stent placement. Methods: Five databases (PubMed, Embase, Cochrane Central Register of Controlled Trials, Web of Science, and China National Knowledge Infrastructure) were searched for randomized controlled trials and observational studies up to April 2020. Results: The mean difference in survival time was 54.87 days (95% confidence interval CI, 34.6-75.14), meaning patients performed with radiofrequency ablation benefit more. Reconstructed Kaplan-Meier data showed improved survival in joint intervention with RFA (hazard ratio, 1.39; 95%CI, 1.34-1.75; P < .001). However, no survival benefit was observed in the extrahepatic distal cholangiocarcinoma. With regard to patency time, the mean difference was 42.88 days (95%CI, 34.02-51.37). Reconstructed Kaplan-Meier data showed improved survival in the radiofrequency ablation treated group (hazard ratio, 1.629; 95%CI, 1.35-1.96; P < .001). Concerning postoperative complications such as abdominal pain, cholangitis and pancreatitis, our analysis did not show a significant difference between the radiofrequency ablation treatment group and the controls. Conclusion: Radiofrequency ablation plus stent resulted in improved survival and stent patency, with longer median survival and patency time than stent alone.
Genomic Predictions for Muscle Yield and Fillet Firmness in Rainbow Trout using Reduced-Density SNP Panels
Authors: Al-Tobasei Rafet , Ali Ali R. , Garcia Andre L. S. , Lourenco Daniela , Leeds Tim , Salem Mohamed 
Published: June 2020

Abstract
Background One of the most important goals for the rainbow trout aquaculture industry is to improve muscle yield and fillet quality. Previously, we showed that a 50K transcribed-SNP chip can be used to detect quantitative trait loci (QTL) associated with muscle yield and fillet firmness. In this study, data from 1,568 fish genotyped for the 50K transcribed-SNP chip and ~774 fish phenotyped for muscle yield and fillet firmness were used in a single-step genomic BLUP (ssGBLUP) model to compute the genomic estimated breeding values (GEBV). In addition, pedigree-based best linear unbiased prediction (PBLUP) was used to calculate traditional, family-based estimated breeding values (EBV). Results The genomic predictions outperformed the traditional EBV by 35% for muscle yield and 42% for fillet firmness. The predictive ability for muscle yield and fillet firmness was 0.19 - 0.20 with PBLUP, and 0.27 with ssGBLUP. Additionally, reducing SNP panel densities indicated that using 500 – 800 SNPs in genomic predictions still provides predictive abilities higher than PBLUP. Conclusion These results suggest that genomic evaluation is a feasible strategy to identify and select fish with superior genetic merit within rainbow trout families, even with low-density SNP panels.
Combined Analysis of the Aberrant Epigenetic Alteration of Colorectal Cancer
Authors: Xing Xiao-liang , Yao Zhi-Yong , Zhang Ti , Zhu Ning , Liu Xingyu , Xiao Liyun , Cheng Kang , Huang Zhi , Liu Yuanwu , Peng Jing 
Published: June 2020

Abstract
BackgroundColorectal cancer (CRC) is the third most common cancer which could be classified as colon adenocarcinoma (COAD) and rectum adenocarcinoma (READ) mainly. Accumulating evidence indicatedmethylations are involved in multiple tumors.MethodsTo know the effect of abnormal methylation in COAD, READ and CRC, we downloaded methylation and mRNA data of COAD and READ from The Cancer Genome Atlas (TCGA) database. And then, we used DESeq2, ChAMP, DAVID 6.8, Cytoscape_3.7.2 and Correlation analysis to identify the potential biomarkers.ResultsWe obtain 12 potential biomarkers (APBB1, CDC42SE2, EIF4E3, FBXO17, FES, GNPNAT1, HSPA1A, OSBPL3, RORC, SALL1, SPEG, and TCF7L1) directly associated with the pathologic TNM of COAD maybe regulated by 28 differential methylations; 2 potential biomarkers (AQP1 and HOXA3) directly associated with pathologic NM maybe regulated by 8 differential methylations; and 15 potential biomarkers (ADAMTSL3, ANXA9, APBB1, AQP1, C2CD4A, CLIP3, DNAJC15, EIF4E3, FAM160A1, GNG4, HLX, HSPA1A, LAYN, NR3C2, and SYT1)directly associated with the pathologic TNM of COAD maybe regulated by 29 differential methylations. Furthermore, weconstruct the network of differential methylation and differential expression genes. In addition, we also obtain 1 methylation (cg18149207), 2 methylations (cg02000808 and cg21134232) and 2 methylations (cg04408595 and cg19413725) associated with the overall survival.ConclusionOur results provide an analysis of theoretical knowledge and clinical outcomes, but more researches are needed to confirm our findings.
Item Response Theory Analysis of the Hospitalized Patients Spiritual Needs Questionnaire
Authors: Hosseini Fahimeh Alsadat , Guardia Joan , Momennasab Marzieh , Yektatalab Shahrzad , Zareiyan Armin , Thomson Brian 
Published: June 2020

Abstract
Background: Hospitalized patients Spiritual Needs Questionnaire (HPSNQ) has been developed in Iran. This paper uses the classical test theory (CTT) to test the validity of the HPSNQ. Using Item response theory, a scale can be constructed with items that can be interpreted regarding the spiritual needs of patients revealing evidence that calls for more effective health interventions regarding meeting the spiritual needs of patients. Analysis of the hospitalized patients Spiritual Needs Questionnaire using Item Response Theory with the aim of providing insight into the functioning of the questionnaire to improve the psychometric robustness of the instrument. Methods: This analytical study was carried out in 2018 on 301 subjects hospitalized in non-acute wards of hospitals in Shiraz, Iran. The analysis was carried out using Item Response Theory: The dimensionality, assessing the items parameters evaluation, and the scale construction were assessed using Cronbach’s alpha, item-to-total correlations and confirmatory factor analysis was done for investigation of dimensionality. The Gradual Response Model of Samejima was used to estimate the item parameters, and the MPLUS software was utilized to analyses the data.Results: Findings revealed that HPSNQ scales are highly unidimensional. In this study, regarding the dimension of “relationship with God”, very high discriminations were observed among all items, whereas the items in “peaceful environment” had relatively low discriminations, hence was not as informative as the items of “relationship to god”.Conclusions: Whereas the psychometric properties of the HPSNQ was generally supported from an IRT perspective, there was also evidence of further improvement for this measure. It seems that the nature of the construct under study, has a significant impact on the items’ discrimination and information value; however, the wording correction or deletion of certain items or even the response options used in this tool may significantly increase the reliability of the tool.
Association Between Commuting Time and Sickness Absence in the Context of China: Transmission Channels and Heterogeneous Effects
Authors: Zicheng Wang , Jiachun Liu , GUO MURONG 
Published: June 2020

Abstract
Background: Most of employees in urban China have experienced a heavy commuting burden，which has become an urgent issue that should be solved in the process of new urbanization strategy. However, not only has the exploration of relationship between the commuting and sickness absence been still scant in China, but also there is no discussion made to analyze the mechanism linking the commuting time and sickness absence. To address these gaps, this study firstly investigates the commuting-absence linkage as well as the potential transmission channel between them.Methods: Using a unique dataset of 2013 Matched Employer-Employee Survey (CMEES) in China, current study apply the zero-inflated negative binomial model to explore the association.Results: Long commute is significantly related with increased sickness absence. A longer commuting is associated with poorer self-rated health status and a higher degree of psychological depression, and it also is highly related with an increase of their BMI index, annual medical expenses as well as the risk of obesity. However, the commuting is not correlated with the overtime length and probability of overtime. Conclusion: The longer commute induces to lower productivities through the increased sickness absence, and the potential linkage of commute-sickness absence is mainly transmitted through their health-related outcomes.
Comparative Study of Preoperative Scores to Predict Lymph Node Metastasis in Patients With Gastric and Colon Cancers: A Case Control Study
Authors: Zheng Xiangjian , Chen Xiaodong , Li Min , Li Chunmeng , Shen Xian 
Published: June 2020

Abstract
Background:Surgery combined with chemo-radiotherapy is a recognized model for the treatment of gastric and colon cancers. Lymph node metastasis determines the patient's surgical or comprehensive treatment plan.The purpose of this study was to compare preoperative scores to better predict lymph node metastasis in patients with gastric and colon cancers.Methods:A total of 768 patients with gastric cancer (312) and colon cancer (462) were enrolled in our study. Preoperative serum markers, immune markers, and clinical tumor characteristics were evaluated by single-factor analysis. Logistic analysis was used to identify independent predictors of lymph node metastasis in patients with gastric and colon cancers. These independent risk factors were integrated into preoperative scores, which was evaluated by receiver operating characteristic (ROC) curves.Results:The result showed that serum markers (CA125, hemoglobin, albumin), immune markers (S100, CD31, d2–40), and tumor characteristics (pathological type, tumor size) were independent risk factors for lymph node metastasis in patients with gastric and colon cancers. The preoperative scores reliably predicted lymph node metastasis in patients with gastric and colon cancers with a higher area under the ROC curve (0.901). Compared to the other independent risk factors, the area under the ROC curve of this indicator was 0.923 and 0.870, for gastric and colon cancers, respectively. Based on the ROC curve, the ideal cutoff value of preoperative scores to predict lymph node metastasis was established to be 287. Conclusion: The preoperative scores is a useful indicator that could be used to predict lymph node metastasis in patients with gastric and colon cancers.