bluuebunny/crossref_metadata_2025_split huggingface dataset:
Samba II PCR testing for COVID-19 in an unselected cohort of pregnant women in the UK
Authors: Xu Ruiling , Pauley Tara Alicia , Missfelder-Lobos Hannah , Haddon Richard John , Gupta Ravindra Kumar , Chong Hsu Phern 
Published: November 2020

Abstract
OBJECTIVE: Asymptomatic carriage of COVID-19 in pregnant women has been reported and could lead to outbreaks in maternity units. We sought to ascertain the impact of rapid isothernal nucleic acid based testing for COVID-19 in an unselected cohort of pregnant women attending our maternity unit. We also assessed the correlation between community prevalence and asymptomatic carriage.DESIGN: Retrospective cohort analysis and literature reviewSETTING: Tertiary hospital in Cambridge, UKPOPULATION: Pregnant women (with gestation age between 12-42 weeks) admitted to a single maternity unit over a 4-week period (07/05/2020-06/06/2020) METHODS: Data were collected using computerised hospital records. Literature searches were performed across multiple repositories. COVID-19 prevalence was extracted from online repositories.RESULTS: NP and OP swabs were obtained from 457/465 women during the study period (98%). The median turnaround time for results was 5.3 hours (interquartile range (IQR) 2.6-8.9 hours). 92% of results were returned within 24 hours. In our cohort, only one woman tested positive, giving a screen positive rate of 0.22% (1/457; 95% confidence interval: 0.04-1.23%). One woman who tested negative developed a fever postnatally following discharge but was lost to follow-up. From our literature review, we did not find any correlation between asymptomatic carriage in pregnant women and the reported regional prevalence of COVID-19. CONCLUSIONS: Testing using the SAMBA-II machine was acceptable to the vast majority of pregnant women requiring admission and had a low turnaround time. Asymptomatic carriage is low, but not correlated to community prevalence rates. Screening pregnant women on admission will remain an important component in order to minimise nosocomial infection.
Characterizing Environmental Inequalities Using Integrated Exposure Assessment and Spatial Approach
Authors: Caudeville Julien , REGRAIN Corentin , TOGNET Frederic , BONNARD Roseline , GUEDDA Mohammed , BROCHOT Celine , BEAUCHAMP Maxime , LETINOIS Laurent , MALHERBE Laure , MARLIERE Fabrice , LESTREMAU Francois , CHARDON Karen , BACH Veronique , ZEMAN Florence Anna 
Published: November 2020

Abstract
BackgroundAt a regional or continental scale, the characterization of environmental health inequalities (EHI) expresses the idea that populations are not equal in the face of pollution. It implies the analysis in order to identifying and managing areas at risk of overexposure where increasing risk to human health is suspected. The development of methods is a prerequisite for the implementation of public health actions aimed at the protection of populations.MethodsThis paper presents the methodological framework developed by INERIS (French national institute for industrial environment and risks) to identify a common framework for conceptualizing and operationalizing environmental exposures as an important step towards articulating a science of EHI. We develop an integrated exposure assessment approach capable to integrate the multiplicity of exposure pathways from various sources, through a series of models up to the internal exposure.ResultsMeasured data from environmental networks reflecting the actual contamination of the environment are reused to characterize the population's exposure. Sophisticated methods of spatial analysis are applied to include additional information and take benefit from spatial and inter-variable correlation to improve data representativeness and characterize associated uncertainty. Integrated approaches bring together all information necessary for assessing the source-to-human-dose continuum using Geographic Information System, multimedia exposure and toxicokinetic model. ConclusionThis framework could be used for many purposes, such as mapping EHI, identifying vulnerable populations and determinants of exposure to manage and plan remedial actions and assessing spatial relationships between health and environmental to identify factors that influence the variability of disease patterns.
Removal of Cu (II) ion from Ezana (Meli) Gold Mining Wastewater Using Eucalyptus Bark as Adsorbent
Authors: Hagos Mebrahtom , Yimam Abubeker , Gebru Kibrom Alebel 
Published: November 2020

Abstract
This study investigated the potential use of Eucalyptus Bark (EB) powder as an adsorbent in batch mode experiments for removal of Cu2+ from Ezana (Meli) wastewater. The discharge of untreated gold mining wastewater contaminated by Cu (II), which is threatening ecosystems and carcinogenic to the human. Since the removal by using adsorption method is cost effective and environmentally friendly, it has been widely studied by many researchers. Characterizations of Eucalyptus Bark were analyzed using proximate analysis, Fourier transform infrared (FTIR) and X-ray diffractometer (XRD). Various characterization techniques showed that the effluent discharged from the factory contains: total suspended solid (TSS), turbidity, Electrical conductivity (EC), Total dissolved solid (TDS), COD, Temperature, pH, cyanide WAD with <11°C (ppm). Atomic absorption spectroscopy study indicated that heavy metals found in the wastewater were in the order Fe2+> Cu (II) >Pb (II) >Mn> Cr (VI) >Zn > Co > Ni > Cd in ppm. The selected parameters were pH, adsorbent dosage and contacting time. The highest percentage of Cu (II) removal achieved was 92%. In this study, the adsorption data were well-fitted to the Langmuir isotherm model.
Genetic Diversity, Population Structure Analysis Using Ultra-High Throughput Diversity array Technology (DArTseq) in Different Origin Sesame (Sesamum	indicum L)
Authors: NEGASH TEWODROS TESFAYE , TESFAYE KASSAHUN , WAKEYO GEMECHU KENENI , ZIYOMO CATHRINE 
Published: November 2020

Abstract
BackgroundSesame is an important oil crop widely cultivated in Africa and Asia continent. Characterization of genetic diversity and population structure of sesame genotypes in these continents can be used to designing breeding methods. In the present study, 300 sesame genotypes comprising 209 local, and 75 exotic collection, and 16 released varieties provided from the Ethiopian Biodiversity Institute and research centers were used in the present study.ResultsThe panel was genotyped using two ultra-high-throughput diversity array technology (DArT) markers (silicoDArT and SNP). Both markers were used to identify the genetic diversity and population structure of sesame germplasm. A total of 6115 silicoDArT and 6474 SNP markers were reported, of which 5002 silicoDArT and 4638 SNP markers were screening with quality control parameters. The average polymorphic information content values of silicoDArT and SNP markers were 0.07 and 0.08, respectively. For further analysis, the allele frequency for each SNP site was calculated and purified with MAF < 0.01 and left 2997 high-quality SNPs evenly distributed across the whole genome that could be used for subsequent analysis. All genotypes used in this study were descended from eight 8 geographical origins. The genetic diversity analysis showed that the average nucleotide diversity of the panel was 0.14. Considering the genotypes based on their geographical origin, Africa collections (0.21) as a whole without Ethiopian collection was more diverse than Asia and when further portioned Africa, North Africa (0.23) collection was more diverse than others, but at the continent level, Asia (0.17) was more diverse than Africa (0.14). The genetic distance among the sesame populations was ranged from 0.015 to 0.394, with an average of 0.165. The sesame populations was clustered into four groups. The structure analysis divided the panel into four subgroups and 21 genotypes were clustered as an admixture. These indicates genotypes from the same origin didn’t classify properly on the premise of the country of origin. ConclusionsThe genetic diversity and population structure revealed in this study should guide the future research work to design association studies and the systematic utilization of the genetic variation characterizing the sesame panel.
Population Genetic Diversity and Structure of Threatened Magnolia Species in Western Mexico
Authors: Muñiz-Castro Miguel Angel , Lopez-Barrera Gabriela , Ochoa-Zavala Maried , Castro-Felix Patricia , Vazquez-Garcia Jose Antonio , Oyama Ken 
Published: November 2020

Abstract
Background: Genetic diversity is needed to preserve the capability of a species to survive to environmental changes. Due to the presence of small isolated populations, relict species such as Magnolia are at an elevated extinction risk. In recent years, many new species of Magnolia have been described in Mexico, each one classified by its category of risk. To achieve conservation, knowledge of their basic level of biological diversity is essential to design adequate conservation plans and avoid the negative consequences of genetic loss. Here, we implemented nuclear microsatellite markers to assess 13 populations of three new species of Magnolia that were all previously considered to be Magnolia pacifica. We aimed to evaluate the genetic agreement with the distinction of these three different morphological species (e.g., their species integrity) and to determine their levels of genetic diversity and their geographic distribution to propose conservation strategies. Results: We found high levels of genetic diversity compared to other Magnolia species with no sign of inbreeding. We found a small effective population size and a prevalence of bottlenecks in some populations. The patterns of genetic subdivision did not support the current morphological distinction of three different species. Instead, we suggest that the genetic structure pattern is the result of historical connectivity and the continuous natural fragmentation of the forest. Thus, an isolation by distance pattern may have had an important role in shaping allele frequencies, producing local genetic differences. Conclusions: We argue that a major threat underlies the actual trends of habitat loss, which can directly impact the loss of genetic diversity in the current adult individuals and consequently, increase the risk of extinction in further generations. For conservation purposes, we suggest combining in situ and ex situ conservation of populations with the maintenance of connectivity among the local populations.
Close up to the Surface: Reflections on a Preliminary Forensic Study of Four Chinese Bronze Mirrors from a Hong Kong Private Collection
Authors: Liang Jiafang , Parker Quentin 
Published: November 2020

Abstract
This article presents an objective, evidence based, forensic study undertaken within the HKU Architectural Conservation Laboratory (ACLab) and Department of Physics conducted on 4 very different bronze mirrors from a private collection. They nominally cover the period from the Warring States (475-221BC), Han (206 BC to 220AD) and later Song (960-1279AD) dynasties. Comprehensive, mostly non-invasive, analytical methods and techniques were applied in this endeavour. These included surface microscopy of tool marks, patina, corrosion and any residual archaeological evidence. Ultraviolet radiation examination, chemical spot testing and polarised light microscopy of ground-up patina samples was also undertaken. More sophisticated “pXRF” X-ray fluorescence and “MARS” tomographic X- ray scanning analysis of the bronze alloy, corrosions and any earthen encrustations were also performed. This was all done to uncover as much forensic evidence as possible concerning these unprovenanced bronze mirrors. The combined results have revealed key metallurgical information of those four mirrors along with surface patina morphology and details of the corrosion and residual surface archaeology. A database on the physical condition of these 4 mirrors has been established and burial/treatment history revealed. Mirrors 1 and 2 appear to have been heavily cleaned, polished and treated with abrasives in modern times. Both of them but mirror 2 in particular have some problematic corrosion and inconclusive alloy composition. Mirror 3 and 4 both have archaeological evidence and no contrary forensic data that questions their authenticity. The issue of the possible authenticity of an object when archaeological context is lacking is discussed but this was not the ultimate goal of this research. The key aim is to establish what can be learnt from technological forensic investigation when studying bronze mirrors without archaeological context and records, and what firm evidence can be extracted from such close forensic examination to shed light on their true nature.
A Risk Assessment Tool for Resumption of Research Activities During the COVID-19 Pandemic
Authors: Simkovich Suzanne M , Thompson Lisa M. , Clark Maggie , Balakrishnan Kalpana , Bussalleu Alejandra , Checkley William , Clasen Thomas , Davila-Roman Victor , Diaz-Artiga Anaite , Fuentes Lisa de las , Harvey Steven , Kirby Miles , Lovvorn Amy , McCollum Eric , Peel Jennifer , Quinn Ashlinn , Rosa Ghislaine , Underhill Lindsay , Williams Kendra , Young Bonnie , Rosenthal Joshua 
Published: November 2020

Abstract
Rationale: The spread of severe acute respiratory syndrome coronavirus-2 has suspended many non-COVID-19 related research activities. Where restarting research activities is permitted, investigators need to evaluate the risks and benefits of resuming data collection and adapt procedures to minimize risk. Objectives: In the context of the multicountry Household Air Pollution Intervention (HAPIN) trial, we developed a framework to assess the risk of each trial activity and to guide protective measures. Our goal is to maximize integrity of reseach aims while minimizing infection risk based on the latest understanding of the virus. Methods: We drew on a combination of expert consultations, risk assessment frameworks, institutional guidance and literature to develop our framework. We then systematically graded clinical, behavioral, laboratory and field environmental health research activities in four countries for both adult and child subjects using this framework. Results: Our framework assesses risk based on staff proximity to the participant, exposure time between staff and participants, and potential aerosolization while performing the activity. One of of four risk levels, from minimal to unacceptable, is assigned and guidance on protective measures is provided. Those activities which can potentially aerosolize the virus are deemed the highest risk. Conclusions: By applying a systematic, procedure-specific approach to risk assessment for each trial activity, we can compare trial activities using the same criteria. This approach allows us to protect our participants and research team and to uphold our ability to deliver on the research commitments we have made to our participants, local communities, and funders. The trial is registered with clinicaltrials.gov (NCT02944682).
Innovation Research of the"Internet plus" Volleyball Teaching System in the Context of the Information Age
Authors: Tian Yang 
Published: November 2020

Abstract
Due to the rapid development of internet technology, big data, artificial intelligence, multimedia and other new technologies are updated rapidly, and all walks of life have an increasingly urgent demand for information technology. As a base for teaching and cultivating talents, schools should adapt to the development of the times and make full use of information technology to carry out educational activities. For example, "Internet plus" can be used for innovation in school teaching mode. This paper mainly talks about the innovation research of "Internet plus" volleyball teaching system in the context of the information age. This paper analyzes the influence of "Internet plus" on the teaching mode in universities and colleges, and studies how to use the "Internet plus" to change the teaching mode of Volleyball in universities. The results of this paper show that the "Internet plus" volleyball teaching is a new teaching system that is united and common. It helps to break the traditional volleyball teaching mode in universities and colleges, and improve the single teaching form, the limitation of time and space, and so on. The confidence interval of SPSS17.0 is 0.96. The use of multimedia in the "Internet plus" volleyball teaching system improves the volleyball performance of students, and promotes the smooth progress of Volleyball Teaching in Colleges and universities.
Method for Monitoring and Increasing the Quality of Experience in Cellular Networks Based on Interconnections Between Key Quality Indicators Using Spline-Approximation
Authors: Azzeh Jamil Al , Odarchenko Roman , Abakumova Anastasiia , Bondar Serhii 
Published: November 2020

Abstract
Sustainable and balanced development of the communications industry is inextricably linked with increasing numbers of subscribers and services provided. The current level of development of the cellular services market, qualitative changes in the means and methods of providing services, and the increased volume and diversity of information circulating in cellular networks have evoked the need for service quality assessment system improvement. To maintain competitiveness, the main efforts of operators are aimed at improving quality and increasing the service life of subscribers in the network by ensuring the required level of customer satisfaction in high quality services through a system of organizational-technical and socio-economic measures to bring the achieved level of quality of service (QoS) provision in accordance with the existing, emerging, or projected needs of subscribers. These circumstances determine the relevance of the studied tasks related to the assessment and management of the quality of cellular services and the development of approaches to their solution.To achieve improved QoS provision, a functional relationship between network parameters has been established, i.e., the impact of key performance indicators on a key quality indicator through the use of cubic Hermitian splines (CHS) has been determined. The use of splines, as a signal model, can significantly improve the quality of signal processing due to the continuity of values and partial derivatives in the joints of spline gluing. CHS are characterized by calculation simplicity, as they provide high speed computing, which, in turn, is important for real time work when processing large data sets. Experimentally, it was shown that the use of splines allows for the ability to calculate statistical estimates of the required parameters of spline approximations, but also their confidence intervals, which increases the accuracy and probability of further calculations and is a distinct advantage of this approachThe methods of service quality management through machine learning have been improved, which are effective tools for modeling integrated indicators of communication services quality control, monitoring their condition in terms of final copies of services, determining the causes of degradation, and reporting. It monitors the service performance provided by the cellular operator. In addition, the method checks the readiness and availability of services, detects network nodes through which quality degradation occurs, collects various quality metrics, and compares them with pre-installed quality indicators.
Paeonol Ameliorates Inflammation and Cartilage of Chondrocytes in Osteoarthritis by Upregulating SIRT1
Authors: Shang Peng , Liu Ying , Jia Junqing 
Published: November 2020

Abstract
Objective: To explore the possible role of paeonol on chondrocyte inflammation and cartilage protection in osteoarthritis (OA).Methods: Primary chondrocytes were isolated from rat stifle joints, and were identified through toluidine blue staining and immunofluorescence staining of type II collagen. The chondrocytes were transfected with sh-SIRT1 or/and paeonol (0, 20, 50, 100, 200, 1000 mg/L) before OA modeling induced by IL-1β. ELISA determined the expressions of TNF-α, IL-17 and IL-6, and apoptotic rate was examined by flow cytometry. qRT-PCR and Western blot quantified the expressions of MMP-1, MMP-3, MMP-13, TIMP-1, cleaved-caspase-3, Bax, Bcl-2, and the proteins related to NF-κB pathway.Results: Increases in TNF-α, IL-17, IL-6, MMP-1, MMP-3 and MMP-13 and decrease in TIMP-1 were found in IL-1β stimulated chondrocytes. The apoptotic rate as well as the expressions of cleaved-caspase-3 and Bax was up-regulated, and Bcl-2 expression was suppressed in response to IL-1β treatment. NF-κB pathway was activated in IL-1β-stimulated chondrocytes. Paeonol enhanced SIRT1 expression to inactivate NF-κB pathway, thus ameliorating the secretion of inflammatory cytokines, extracellular matrix degradation and chondrocyte apoptosis.Conclusion: Paeonol inhibits IL-1β induced inflammation and extracellular matrix degradation in chondrocytes through up-regulating SIRT1 and suppressing NF-κB pathway.
Benign Clinical and Epidemiological Outcomes-Associated Factors of COVID-19 from a Solved Epidemic with a Low Case Fatality Rate
Authors: Hu Gang , Liu Jian , He Peng Yuan , Ouyang Li Juan , Zou Bao Jia , Peng Xiao Mou 
Published: November 2020

Abstract
Background: Since December 2019, COVID-19 has been confirmed in more than18.8 million patients and leads to 0.70 million deaths worldwide. The mortality and disease severity predictors of COVID-19 have been investigated in many studies. However, they are based on early or partial datasets from high epidemic areas. Here, we retrospect benign clinical and epidemiological outcomes-associated factors from a solved epidemic in a low epidemic area. Methods: All 98 laboratory-confirmed COVID-19 patients in a local epidemic (Zhuhai, China) from January 17, 2020 to March 10, 2020 were enrolled. Data were updated until all patients having final outcomes. Results: Patients were all hospitalized. The case fatality rate was 1.0%. There were no local secondary infection cases. The median age was 46.3 years. Underlying diseases were found in 33.7% patients. The severe/critical rate was 19.4%. The mean period from disease onset to admission was 4.4 days. Compared with serious/critical cases, mild/common cases on admission were much younger, lacks of comorbidities and normal in functions of vital organs and indicators of secondary bacterial infections. The lymphocyte counts in serious/critical cases began to be significantly lower 3 days before their identification dates. The absence of lymphopenia before the eighth day from disease onset can exclude the possibility of 78.5% to be serious/critical ill. Most patients (88.8%) received antiviral treatments. Early antiviral treatment significantly shortened the viral RNA-negative conversion time. The delayed antiviral treatment was associated with critical patients.Conclusions: Younger age, lack of aging-related diseases and early hospitalization of all patients to conduct antiviral treatment and prevention of secondary epidemic were the important benign clinical and epidemiological outcomes-associated factors of COVID-19. In combating COVID-19, the active intervention strategies are crucial in low epidemic areas and the continuous monitoring of lymphocytes may be useful to sort patients reasonably in high epidemic areas.