bluuebunny/crossref_metadata_2025_split huggingface dataset:
Improved subspecies identification in clinical Mycobacterium abscessus complex isolates using whole genome sequencing
Authors: Lin Dachuan , Sun Ruifeng , Tan Yaoju , Wang Jing , Chen Xinchun , Li Yun 
Published: March 2020

Abstract
BackgroundMycobacterium abscessus complex, which is frequently reported to cause a variety of skin and soft tissues diseases in humans, is composed of three subspecies, namely, M. abscessus subsp. abscessus, M. abscessus subsp. massiliense and M. abscessus subsp. bolletii. Currently, the differentiation of these three subspecies in clinical isolates still largely depends on single gene identification methods, including the genes hsp65 and 16s, with limited accuracy.ResultsThis study confirmed the limitations of the single gene-based method of subspecies identification. We performed a comprehensive analysis of MABC genomes in the NCBI database and tried to build an accurate and user-friendly identification method. Here, we describe an improved assay for Mycobacterium abscessus complex rapid identification using WGS data, based on the identities of rpoB, erm(41) and rpls. Comprehensive analysis has been performed to compare our software results with the traditional method. The results showed that the method built in this study could achieve 100% identification of subspecies for the Mycobacterium abscessus complex in a public genome database (893 genomes from the NCBI database and 6 clinical isolates from this study). This software can be easily integrated into a routine workflow to quickly and precisely provide subspecies-level identification and discrimination of different MABC subspecies in clinical isolates by WGS.ConclusionsThis assay will facilitate accurate molecular identification of species from the MABC complex in a variety of clinical specimens and diagnostic contexts.
Bilateral simultaneous total hip arthroplasty: comparison of treatment outcome between the direct anterior approach and the posterolateral approach
Authors: Feng Shuo , Sun Jian-Ning , Zhang Yu , Zhang Ye , Chen Xiang-Yang , Zha Guo-Chun 
Published: March 2020

Abstract
Background There are many surgical approaches for total hip arthroplasty. In recent years, direct anterior approach (DAA) has been highly praised by many scholars, and it has been widely reported that it has a good curative effect, such as fast recovery. Whether the surgical results and patient satisfaction can reach or exceed the traditional posterolateral approach has been controversial. We hypothesized that the treatment outcome of the direct anterior approach (DAA) approach is superior to the traditional posterolateral approach (PLA). Methods From January 2015 to April 2017, 20 patients (40 hips) were randomly divided into posterolateral approach (PLA) group and direct anterior approach (DAA) group. Record the operation time on both sides, postoperative drainage, prosthesis position, and complications, the functional recovery of hip joint was evaluated by D'Aubigne-Postel score, and the postoperative pain was evaluated by VAS score. Gait parameters were measured before surgery and at 3 and 6 months after surgery, and the patients were asked which side they preferred subjectively after surgery. Results When compared with the PLA group, the DAA group had a shorter incision length (11.4 vs 14.72 cm, P<0.001), shorter intraoperative blood loss (184.05vs 219.00 mL, P<0.001), shorter postoperative drainage volume (105.35 vs 154.10 mL, P<0.001), and lower VAS scores. However, the PLA had shorter operative times (82.20 vs 67.3 min, P<0.001). There was no significant difference in acetabular inclination (39.72 vs 40.92 °, P=0.069), and acetabular anteversion (17.41 vs 17.69°, P=0.663) between the two groups. Joint function recovery: The D'Aubigne-Postel scores of the hip joints in the DAA group vs PLA group at 1, 3, and 6 months after surgery were (8.2, 11.5, 16.5) vs (7.65, 11.45, 16.9). The difference in the 1, 3month was statistically significant (P=0.012, P=0.038), however, this difference disappeared in the 6th month(P=0.072). Conclusions Compared with the PLA, the DAA has the advantages of shorter operation time, smaller incision, less bleeding, less pain, better gait performance and faster recovery of joint function, but patients subjectively preferred the traditional PLA.
Incidence and Risk Factors of Postoperative Delirium Following Total Knee Arthroplasty: A Retrospective Nationwide Inpatient Sample Database Study
Authors: Wang Jian , Yang Qinfeng , Xu Yichuan , Chen Yuhang , Lian Qiang , Zhang Yang 
Published: March 2020

Abstract
BackgroundPostoperative delirium is a common complication following major surgeries, causing a variety of adverse effects. However, the incidence and risk factors of delirium after total knee arthroplasty (TKA) has not been well studied using a large-scale national database. MethodsA retrospective database analysis was performed based on Nationwide Inpatient Sample (NIS) from 2005-2014. Patients who underwent TKA were included. Patient demographics, comorbidities, length of stay (LOS), total charges, type of insurance, in-hospital mortality, and medical and surgical perioperative complications were evaluated.ResultsA total of 1,228,879 TKAs were obtained from the NIS database. The general incidence of delirium after TKA was 1.00%, which peaked in the year 2008. Patients with delirium after TKA presented more comorbidities, increased LOS, extra hospital charges, wider coverage of medicare, and higher in-hospital mortality (P<0.0001). Delirium following TKA was associated with medical complications during hospitalization including acute renal failure, acute myocardial infarction, pneumonia, pulmonary embolism, stroke, and urinary tract infection. Risk factors of postoperative delirium included advanced age, neurological disorders, alcohol and drug abuse, depression, psychoses, fluid and electrolyte disorders, diabetes, weight loss, deficiency and chronic blood loss anemia, coagulopathy, congestive heart failure, chronic pulmonary disease and pulmonary circulation disorders, peripheral vascular disorders, renal failure, and teaching hospital. ConclusionsA relatively low incidence of delirium after TKA was identified. Postoperative delirium of TKA was associated with increased comorbidities, LOS, total charges, coverage of medicare, mortality and medical perioperative complications. It is of benefit to study risk factors of postoperative delirium to ensure the appropriate management and moderate its consequences.
Association of Interleukin-16 rs4778889 T&gt;C Gene Polymorphism with Cancer Risk: a Meta-analysis
Authors: Lei Qin , Jiawen Zhao , Yutong Zhao , Chenjun Ma , Chaobin Li , Jiwen Cheng , Chengyang Li 
Published: March 2020

Abstract
BACKGROUND: Rs4778889 T>C is one of the single nucleotide polymorphisms (SNPs) in interleukin-16 (IL-16). As a growth factor, IL-16 might play a significant impact on cancer formation. Several studies have investigated the relationship between IL-16 rs4778889 T>C gene polymorphisms and cancer risk, but the results are contradictory. We conducted a meta-analysis on the association between IL-16 rs4778889 T>C gene polymorphism and cancer risk. METHODS: Twelve case–control studies with 3066 cases and 4433 controls from Web of Science, PubMed, and Embase databases were included. The data was analyzed using the STATA software and the combined odds ratio (ORs) and the corresponding 95% confidence interval (CIs) were used to identify the correlation strength. RESULTS: Our results show that no significant associations were observed between the IL-16 rs4778889 T>C gene polymorphisms and cancer risk in all genetic models (C vs. T: OR=1.06, 95% CI: 0.90–1.26, Ph<0.001; CC vs. TT: OR=1.07, 95% CI: 0.71–1.62, Ph<0.001; CT vs. TT: OR=1.07, 95% CI: 0.91–1.26, Ph=0.002; CC+CT vs. TT: OR=1.08, 95% CI: 0.90–1.30, Ph<0.001; CC vs. CT+TT: OR=1.04, 95% CI: 0.73–1.50, Ph=0.001). Subgroup and sensitivity analyses also were performed. CONCLUSIONS: The results of this meta-analysis indicate that there are no significant associations between IL-16 rs4778889 T>C gene polymorphisms and cancer risk. To verify these results, further studies with larger sample size and multiracial populations are needful. Keywords: IL-16, polymorphisms, cancer, meta-analysis
MENA promotes esophageal squamous cell carcinoma cell invasion and migration via MMP-2, MMP-9 and AKT activation
Authors: Li Yueheng , Gao Na , Li Jing , Gao Zhengfan , Yang Zhenzhen , Dang Jinghan , Hou Guiqin , Liu Hongtao , Fan Tianli 
Published: March 2020

Abstract
Background: Esophageal squamous cell carcinoma (ESCC) is a fatal disease with poor prognosis. The predominant reason for ESCC-related death is metastasis caused by tumor cell invasion. Human MENA protein is a member of Ena/Vasp family, which plays a critical role during tumor cell invasion. However, the biological effect of MENA in ESCC cell lines remains unclear Methods: In this study, fluorescent quantitative real-time PCR (qRT-PCR) were conducted to detect the mRNA expression of MENA in tumor and para-cancer tissue, CCK-8 assay and clone formation assay were conducted to evaluate cell proliferation activity, Transwell assay and wound-healing assay were conducted to detect the changes of cell invasion and migration capacity, siRNA and MENA expression vector were constructed to explore biological function of MENA in ESCC cell lines. Western blot analysis were conducted to detect the expressions of MENA , molecular markers of epithelial-mesenchymal transition (EMT), Akt, p-Akt, MMP-2 and MMP-9 respectively in ESCC cell line. Results: The qRT-PCR experiment results showed that MENA expression in ESCC tissue of 35 patients was relatively higher than that in tissue adjacent to cancer. CCK-8 assay suggested that tumor cell proliferation capacity was suppressed followed by the knockdown of MENA expression in Mena high ESCC cell TE13 and was potentiated by the overexpression of MENA in Mena low ESCC cell TE1. Transwell assay and wound healing assay demonstrated that interfering in MENA could inhibit TE13 cells invasion and migration capacity by affecting the expressions of Matrix metalloproteinase-2(MMP-2) and Matrix metalloproteinase-9 (MMP-9), in contrast, overexpression of MENA in Mena low ESCC cell TE1 could promote invasion and migration by up-regulated expression of MMP-2 and MMP-9. Western blot analysis indicated that interfering of MENA expression could affect EMT-related molecular markers (E-cadherin, N-cadherin, Snail, Slug), Akt and p-Akt Conclusions: Our study reveal that MENA could promote the ESCC cell invasion and migration by upregulate MMP-2, MMP-9 expression and Akt activation. Meanwhile, interfering of MENA expression could affect EMT in ESCC cells. This indicated that MENA may be a potential molecular therapeutic target for ESCC metastasis
Germline Variation Networks in the PI3K/AKT Pathway Corresponding to Familial High-incidence Lung Cancer Pedigrees
Authors: Lin Huan , Zhang Gong , Zhang Xu-chao , Lian Xin-lei , Zhong Wen-zhao , Su Jian , Chen Shi-liang , Wu Yi-long 
Published: March 2020

Abstract
Background: There were scarcely germline variants of familial lung cancer(LC) identified. We conducted an study with whole-exome sequencing of pedigrees with familial lung cancer to analyze the potential genetic susceptibility. Methods: Probands with the highest hereditary background were identified by our large-scale epidemiological study and five ones were enrolled as a learning set. The germline SNPs of other five similar probands, four healthy individuals in the formerly pedigrees and three patients with sporadic LC were used as a validation set, controlled by three healthy individuals without family history of any cancer. The network of mutated genes was generated using STRING-DB and visualized using Cytoscape. Results: Specific and shared somatic mutations and germline single-nucleotide polymorphisms (SNPs) were not the shared cause of familial lung cancer. However, individual germline SNPs showed distinct protein-protein interaction network patterns in probands versus healthy individuals and patients with sporadic lung cancer. SNP-containing genes were enriched in the PI3K/AKT pathway. These results were validated in the validation set. Furthermore, patients with familial lung cancer were distinguished by many germline variations in the PI3K/AKT pathway by a simple SVM classification method. It is worth emphasizing that one person with many germline variations in the PI3K/AKT pathway developed lung cancer during follow-up. Conclusions: The phenomenon that the enrichments of SNP mutations in the PI3K/AKT pathway might be a major predictor of familial susceptibility to lung cancer.
Microstructural, Tribology, and Densification Studies of Spark Plasma Sintered Titanium Matrix Composites Reinforced with Silicon Carbide
Authors: Odetola Peter Ifeolu , Popoola Abimbola Patricia , Ajenifuja Emmanuel , Popoola Olawale 
Published: March 2020

Abstract
Spark plasma sintered titanium matrix composites (TMCs) with varying SiC contents were fabricated at 900 o C, 150 o C/min, 30 MPa, and with 5 min of holding time, and were studied for structural, mechanical and tribology performances. The phase identification and microstructure analysis of the sintered specimens were examined using X-ray diffraction and scanning electron microscope equipped with EDS. The results indicate that influence of the varied SiC content dictate the physical properties of the sintered TMCs. The densification study showed that relative density was inversely related, while Vickers hardness value was directly proportional (238.84 Hv at 1 wt. % SiC and 361.81 Hv 6 wt. % SiC). The tribology study showed that both wear rate and coefficient of friction have inverse relationship while wear resistance has a direct trend with the composite reinforcement. Sample TiNiAl – 6 wt. % SiC, with the optimum composition had the best wear performance under the constant load of 20 N. This wear performance can be attributed to the good interfacial bond formed between the TiNiAl matrix and the SiC reinforcement in the developed composites contributed from the synergetic processing conditions of the SPS process.
Rotating night shift work, exposure to light at night, and glomerular filtration rate: baseline results from a Chinese occupational cohort
Authors: Zhang Shengkui , Wang Yongbin , Wang Zhende , Wang Han , Xue Chao , Li Qinglin , Li Xiaoming , Guan Weijun , Yuan Juxiang 
Published: March 2020

Abstract

Background The misalignment between the circadian clock and behavioral cycles has been implicated in pathogenesis of many diseases. However, whether the kidneys are also more prone to disease in the circumstances of chronic circadian rhythms disruption due to night shift work and light at night (LAN) is unclear. The main purpose of this study is to examine the association between rotating night shift work, exposure to light at night, and glomerular filtration rate among steelworkers in north China. Methods A total of 6869 participants, aged 22 to 60 years, were included in this study. Multivariable logistic regression was used to examine the association between night shift work, the brightness of bedroom ambient light at night (LAN), and estimated glomerular filtration rate (eGFR) with adjustment for potential confounders. The relationship between duration of night shift work (continuous), cumulative number of night shifts (continuous), and eGFR were also examined using restricted cubic spline models. Results Long duration of night shift work (≥29 years) had elevated odds of decreased eGFR (OR, 1.39, 95% CI 1.10–1.75) compared with day work after adjustment for potential confounders. Negative associations between duration of night shift work, cumulative number of night shifts, and eGFR (mL/min/1.73 m 2 ) were observed in RCS models. No significant associations were observed among the different brightness of bedroom ambient light levels: middle level (OR, 0.90, 95% CI 0.77–1.05), lightest level (OR, 0.94, 95% CI 0.75–1.17), and decreased eGFR compared with the darkest level. Conclusion The increased duration of night shift work and cumulative number of night shifts among night shift workers, but not the brightness of bedroom ambient LAN, are associated with a slight decline of renal function among steelworkers.
Antimicrobial susceptibility of selected Essential Oils and their compounds against Streptococcus suis
Authors: Aguiar F.C. de , Solarte A.L. , Gómez Lidia , Galan-Relaño A. , Luque I. , Tarradas C. , Rodriguez-Ortega M.J. , Huerta B. 
Published: March 2020

Abstract
Background: Streptococcus suis is an emerging zoonotic pathogen causing different diseases, in both humans and pigs. Generally, the control of diseases caused by this pathogen is based on antimicrobial therapy, but the development of bacterial resistance has led to look for new options. In this sense, the Essential Oils (EOs) constitute an alternative to the use of conventional antimicrobials. The activity of oregano, cinnamon, common thyme and red thyme EOs and their main active compounds (carvacrol, cinnamaldehyde and thymol) against 56 S. suis isolates from pigs (n=50) and human (n=6) was determined by broth microdilution. MIC50-90, MBC50-90 and the bactericidal index (MBC/MIC) were calculated. Also, the time-kill curve of each product against the S. suis P1/7 European reference strain was determined. Results: No differences in the MIC or MBC values were observed between all the tested products, which suggest a homogenous behaviour of S. suis, independently of their origin, organ of isolation or resistance profile. All the products showed a concentration-dependent and time-dependent killing activity and achieved the virtual eradication of S. suis (reduction of ≥ 4 log10) at suprainhibitory (2x-4x MIC) concentrations within the first 5 minutes of exposure, except cinnamaldehyde, that showed only bacteriostatic effect (reduction of < 3 log10). Conclusions: While all the tested products demonstrated an antimicrobial activity, red thyme and cinnamon followed by thymol showed the best results. It suggests that these products would be recommended as antimicrobials in veterinary medicine for the control of this zoonotic pathogen, although more pharmacology, toxicology, tolerability and formulation studies are necessary.
Late Initiation Of Breastfeeding Is A Risk Factor For Early Interruption Of Exclusive Breast Feeding A Case -control Study At Dollo Ado Refuge Camp, Somali Region, Ethiopia.
Authors: Gebeyehu Dawit Getachew , Haftu Desta 
Published: March 2020

Abstract
Background: Globally , nearly three fourth of infants less than-six was not exclusively-brestfeed. While in Ethiopia, the proportion was only slightly above half. In refugee, setting life is not stable, due to lack of infrastructure and adequate health care service mother and infant face many health related problems, like interruption of exclusively breastfeed which intern lead them to malnutrition and impaired growth.Objectives: To assess the Determinants of Interruption of Exclusive Breastfeeding among mother who have infant less than six month at Dollo Ado Refugees camp, Ethiopia, 2017.Methods: A case -control study design was conduct at Dollo Ado refugee camp from April 05 to 25, 2017. Simple random sampling technique used to select 111 eligible case and 222 controls during the study period. A structured and pretested interviewer administered questionnaire used to collect the data. Logistic regression models fitted to assess significant factor, which determine interruption of Exclusive breast-feeding in the study area.Results: In this study not getting antenatal counseling about infant feeding (A-OR =5.87, 2.63-13.10), postnatal counseling about infant feeding (A-OR= 4.33, 2.7 - 10.78) and presence of problem of breastfeeding (AOR= 10.72, 4.55 - 25.23), late initiation of breastfeeding (AOR= 4.79, 2.28-10.0) were significant determinants of interruption of exclusive breastfeeding.Conclusions : In this study, not getting antenatal and postnatal counseling about infant and young child feeding, problem of breastfeeding, late initiation of Exclusive breastfeeding were significant determinants of interruption of EBF. Planer and manager should focus on provision of mother advice on Infant and Young Child Feeding during Antenatal and Postnatal Care. Health professional should be alert on treating problem of breastfeeding.
Morin-type transition in 5C pyrrhotite
Authors: Haines Charles R.S. , Lampronti Giulio I. , Klooster Wim T. , Coles Simon J. , Dutton Sian E. , Carpenter Michael A. 
Published: March 2020

Abstract
We report the discovery of a low-temperature spin-flop transition in 5C pyrrhotite at ~155 K that is similar to those seen in hematite at 260 K and FeS (troilite) at 440 K. The 5C crystal was produced by annealing a 4C pyrrhotite crystal at 875 K to produce a change in the vacancy-ordering scheme that developed during cooling. The 5C structure is confirmed by single-crystal X-ray diffraction and the stoichiometry and homogeneity by electron microprobe and SEM BSE mapping. Resonant ultrasound spectroscopy (RUS), heat capacity, and magnetization measurements from room temperature down to 2 K are reported. The transition is marked by a steep change in elastic properties at the transition temperature, a peak in the heat capacity, and weak anomalies in measurements of magnetization. Magnetic hysteresis loops and comparison with the magnetic properties of 4C pyrrhotite suggest that the transition involves a change in orientation of moments between two different antiferromagnetic structures, perpendicular to the crystallographic c-axis at high temperatures and parallel to the crystal-lographic c-axis at low temperatures. The proposed structures are consistent with a group theoretical treatment that also predicts a first-order transition between the magnetic structures.
A Study Based on the Correlation Between Slit2/Robo1 Signaling Pathway Proteins and Polymyositis/Dermatomyositis
Authors: Chai Ke-Xia , Chen Yu-Qi , Kong Ling-Shuang , Fan Pei-Lin , Yuan Xia , Yang Jie 
Published: March 2020

Aims:
To investigate the role of Slit2 and Robo1 during the vascular disease
of Polymyositis (PM) / dermatomyositis (DM).

Background :
PM and DM are nonsuppurative inflammatory myopathies that mainly
invade the skeletal muscles.

Objective:
This study attempted to explore the specific mechanism of Slit2/Robo1
signaling pathway proteins during the vascular disease of PM/DM.

Methods:
The mRNA expressions of Slit2 and Robo1 in the muscle tissue were
detected by RT-qPCR between newly-diagnosed PM/DM patients and healthy controls.
The number of Slit2 and Robo1 positive cells in the serial sections of muscle paraffin
tissues was measured by immunohistochemistry in 10 patients with PM, 10 patients with
DM and 20 healthy controls.

Results:
The study results revealed that the mRNA expressions of Slit2 and Robo1 in
muscle tissue in the PM and DM groups were higher than that in the control group
(P<0.05). The positive expression rates of Slit2 and Robo1 in muscle tissue in the PM
and DM groups were 80.0%, 80.0%, 70.0% and 70.0%, respectively. The difference was
statistically significant (P<0.001), when compared to the control group (the positive
expression rates were 0% and 10%, respectively).

Conclusion:
The activation of the Slit2/Robo1 signaling pathway is an important
mechanism leading to the development of PM/DM.
N-(2-(Arylmethylimino)Ethyl)-7-Chloroquinolin-4-Amine Derivatives: A New and Potent Class of Anticancer Agents
Authors: da Silveira Pinto Ligia S. , de Souza Marcus V.N. , Kaiser Carlos R. , Wardell James L. , Wardell Solange M.S.V. 
Published: March 2020

Background:
In this study, we reported the in vitro cytotoxicity activities of a series of
N-(2-(arylmethylimino)ethyl)-7-chloroquinolin-4-amine derivatives against four human cancer cell
lines.

Methods:
Good activities were obtained for compounds having aryl groups = 2-hydroxyphenyl,
pyridinyl, 5-nitrofuran-2-yl and 5-nitrothiophen-2-yl.

Results and Conclusion:
The results indicate that this group of compounds is a good starting point
for the potential discovery of new prototypes against cancer.