bluuebunny/crossref_metadata_2025_split huggingface dataset:
Biosynthesized silver nanoparticles and their genotoxicity
Authors: Shati Ali A. , Elsaid Fahmy G. 
Published: November 2019

AbstractThe human‐pathogenic bacteria have become highly resistant to conventional antibiotics; for this reason, a new biosynthesized nanomaterial might be a solution. The culture filtrate of two isolates of Fusarium oxysporum (14, 17) was used in the biosynthesis of nanosilver (AgNPs). The size of the nanoparticles produced by isolate F14 ranged from 19 to 30 nm, whereas the size of those formed via isolate F17 ranged between 16 and 25 nm. Moreover, the produced bio‐nanosilver was tested against the human‐pathogenic bacteria Proteus vulgaris, Escherichia coli, Staphylococcus aureus, and Klebsiella pneumonia and the outcome results displayed great antibacterial efficacy in a different manner compared with the three different biogenic antibiotics. Collectively, the results depicted that the silver nanoparticles (AgNPs) showed a three and a half times greater activity than the used antibiotics. Differential display reverse transcription‐polymerase chain reaction was used to study gene regulation in the treated E. coli (F14) compared with the nontreated ones. Different upregulated and downregulated genes were observed. The cytotoxicity of the produced AgNPs was examined on rats with an average body weight of 200 g each; these animals were grouped into three different groups. The obtained AgNPs showed very low toxicity on the treated rats in comparison to the control group. The physiological parameters, for example, alanine aminotransferase, aspartate transaminase, albumin, creatinine, and urea in the treated animals were changed within to a lower degree compared with those in the nontreated animals. The current study exhibited that AgNPs might be favorable antibacterial agents, especially against multidrug‐resistant bacteria.
Docosahexaenoic acid suppresses migration of triple‐negative breast cancer cell through targeting metastasis‐related genes and microRNA under normoxic and hypoxic conditions
Authors: Javadian Mahsa , Shekari Najibeh , Soltani ‐ Zangbar Mohammad S. , Mohammadi Ali , Mansoori Behzad , Maralbashi Sepideh , Shanehbandi Dariush , Baradaran Behzad , Darabi Masood , Kazemi Tohid 
Published: November 2019

AbstractThere is insufficient evidence with respect to the effect of the standard anticancer therapeutic agents as well as common dietary supplements on the expression of such genes and microRNAs (miRNAs). Therefore, this study was aimed to study the effect of applying linoleic acid (LA) and docosahexaenoic acid (DHA) fatty acids alone or combined with Taxol on the expression of the matrix metalloproteinase (MMP)‐9, MMP‐2, vimentin, and talin2 genes, tumor‐suppressor miR‐194 and, onco‐miR‐106b in triple‐negative breast cancer cell line, known as MDA‐MB‐231. MDA‐MB‐231 as metastatic breast cancer cell line was cultured and treated using 0.3 μM Taxol, 100 μM DHA, and 50 μM LA for 24 hours, alone or combined with Taxol under the normoxic and hypoxic conditions. Cells were harvested, after RNA extraction and complementary DNA synthesis, analysis of the expression levels of the studied genes and miRNAs was done through the use of the quantitative real‐time polymerase chain reaction (qRT‐PCR). Wound healing assay and Western blot analysis were also performed for confirmation. The results of qRT‐PCR showed that treating the MDA‐MB‐231 cells with DHA caused an increase in the miR‐194 expression and a decrease in the miR‐106b expression, leading to the downregulation of the MMP‐2 and MMP‐9, and vimentin, and upregulation of the talin2 under the normoxic and hypoxic conditions. The results of the wound healing scratch assay revealed that the administration of the DHA and the DHA‐Taxol combination caused the repression of cell migration in comparison with the control groups under the normoxic and hypoxic conditions. The results of the Western blot analysis demonstrated that DHA and the DHA‐Taxol combination caused an increase in the expression of the talin2 protein rather than the control cells under both normoxic and hypoxic conditions. This study showed that DHA has significant antimetastatic effects against the triple‐negative breast cancer cells. DHA could serve as a promising supplementation for suppressing the breast cancer cell migration, especially under the hypoxic condition.
Downregulated microRNA‐92a‐3p inhibits apoptosis and promotes proliferation of pancreatic acinar cells in acute pancreatitis by enhancing KLF2 expression
Authors: Ling Lan , Wang Hai‐Feng , Li Jing , Li Yan , Gu Cheng‐Dong 
Published: November 2019

AbstractAcute pancreatitis (AP) is known worldwide as one of the most common gastrointestinal diseases, prospectively leading to hospitalization coupled with increasing incidence. Several microRNAs (miRNAs) have been reported to be potential biomarkers for pancreatitis. In this study, we verified the hypothesis that miR‐92a‐3p is implicated in the development of AP by controlling the proliferation and apoptosis of pancreatic acinar cells (PACs) through the modulation of the Kruppel‐like factor 2 (KLF2) and inflammatory factors in rats. Initially, we established a rat model of AP and extracted the pancreatic tissues. Then, the positive rate of KLF2 was measured using immunohistochemistry, and the expression of the related genes was determined by rReverse transcription quantitative polymerase chain reaction and Western blot analysis. The cell proliferation and apoptosis were measured by 5‐ethynyl‐2′‐deoxyuridine assay and flow cytometry, and the contents of inflammatory factors were measured using enzyme‐linked immunosorbent assay. AP rats presented with increased miR‐92a‐3p expression as well as decreased KLF2 expression in PACs. The downregulation of miR‐92a‐3p and overexpression of KLF2 led to decline in expression of nuclear factor‐κB (NF‐κB), survivin, tumor necrosis factor‐α, and Bax as well as extent of NF‐κB phosphorylation, contents of inflammatory factors, and apoptosis rate of PACs, but to increased KLF2 and B‐cell lymphoma‐2 levels and proliferation rate of PACs. Collectively, the data obtained from the present study demonstrated that reduced miR‐92a‐3p expression may relieve AP through its suppressive effects on cell apoptosis, inflammatory factors, and facilitatory effects on cell proliferation by enhancing KLF2 expression.
Acute glucose fluctuation induces inflammation and neurons apoptosis in hippocampal tissues of diabetic rats
Authors: Wang Hui , Deng JiuLing , Chen Liang , Ding Ke , Wang Yi 
Published: November 2019

AbstractIt is well‐recognized that glycemic disorders are leading causes of diabetic complications and acute fluctuation of blood glucose and reported more likely being related to oxidative stress, vasculopathy, and other diabetic complications than continuous hyperglycemia in patients with diabetic and animal models. To explore the hypothesis that acute glucose fluctuation (GF) aggravates inflammatory lesions and neuron apoptosis in the hippocampus of diabetic rats. Twenty female GK rats were randomly allocated into a glucose fluctuating group (GK‐GF) and a continuous hyperglycemia group (GK‐CHG) and 10 age‐matched female Wistar rats served as controls. GF was induced in the GK‐GF group by injection with glucose and insulin at different periods of time per day for 6 weeks. Body weight was determined weekly. At the end of the study, blood hemoglobin A1c (HbA1c) and serum lipids were measured. Serum and hippocampus interleukin 1β (IL‐1β), IL‐6, IL‐8, and tumor necrosis factor‐α (TNF‐α) were measured by enzyme‐linked immunosorbent assay and real‐time quantitative reverse transcription‐polymerase chain reaction (qRT‐PCR). Hippocampus Bcl‐2, Bax, Pten, fas, and myc were quantified by qRT‐PCR and Western blot analysis and Mirror Water Maze (MWM) test was performed. We successfully established an animal model with daily GF and a control model with CHG using GK diabetic rats. The GF and CHG rats showed lower weight gain during the 6‐week experimental period with no significant difference in the levels of serum lipids such as total triglyceride, total cholesterol, high‐density lipoprotein cholesterol, and low‐density lipoprotein cholesterol compared with the control rats at the end of the study. Meanwhile, the GF and CHG rats showed higher blood HbA1c levels than that of control rats. MWM trainings tests detected that glucose disorders in GF and CHG rats tend to present longer latencies, more cross times and longer path length compared with those of the control rats, indicating impaired the hippocampus‐regulated behavioral function such as spatial orientating and memory. Importantly, it was found that GF promoted the expression of TNF‐α and IL‐1β in the hippocampus of the GF rats while continuous hyperglycemia in CHG rats had little effect on that. Furthermore, both GF and CHG diabetic rats had abnormal expression of apoptosis‐associated genes in the hippocampus compared with control Wistar rats and neurons apoptosis in GF rats appears to be more severe than CHG rats. Overall, this study confirmed that GF is a more critical factor that would promote the neuron apoptosis and induce inflammation in the hippocampus than continuous hyperglycemia in diabetic animals, which shed new light on the importance of monitoring and administration of blood glucose in the prevention and therapy for diabetes.
LIN28B/IRS1 axis is targeted by miR‐30a‐5p and promotes tumor growth in colorectal cancer
Authors: Tang Mei , Zhou Jing , You Lirui , Cui Zhirong , Zhang Hui 
Published: November 2019

AbstractInsulin receptor substrate 1 (IRS1) is a potential oncogene that has been implicated in several malignant tumors. However, the regulatory mechanism of IRS1 remains to be investigated. The aim of our current study is to unveil the mechanism by which IRS1 exerts functions in tumorigenesis of colorectal cancer (CRC). The expression level of IRS1 was found to be higher in CRC cells in comparison with the normal cell. To determine the role of IRS1 in regulating CRC cellular processes, loss‐of‐function assays were designed and carried out in two CRC cell lines. Both in vitro and in vivo functional assays indicated that silencing of IRS1 suppressed CRC cell survival. Based on bioinformatics prediction and mechanism experiments, IRS1 was identified as a downstream target of miR‐30a‐5p. Furthermore, RNA‐binding protein lin‐28 homolog B (LIN28B) was determined to be a stabilizer of IRS1 messenger RNA. More importantly, LIN28B also acted as a target of miR‐30a‐5p.Through rescue assays, we proved that LIN28B‐stablized IRS1 mediated miR‐30a‐5p‐mediated CRC cell growth. In conclusion, this study revealed that LIN28B and LIN28B‐stablized IRS1 promoted CRC cell growth by cooperating with miR‐30a‐5p.
The personal self of psychotherapists: Dimensions, correlates, and relations with clients
Authors: Orlinsky David E. , Rønnestad Michael Helge , Hartmann Armin , Heinonen Erkki , Willutzki Ulrike 
Published: November 2019

AbstractObjectiveThe personal self of psychotherapists, that is, experiences of self in close personal relationships and its association with therapists' individual and professional attributes is explored. The study aimed to: (a) describe therapists' self‐ratings on specific self‐attributes; (b) determine their dimensionality; (c) explore demographic, psychological, and professional correlates; and (d) assess the convergence with professional self.MethodData from the Development of Psychotherapists Common Core Questionnaire were available for > 10,000 psychotherapists of various professions, theoretical orientations, career levels, and nations.ResultsMost psychotherapists described themselves in close relationships in affirming terms (e.g., warm/friendly), although a substantial minority also described themselves in negative terms. Factor analyses yielded four dimensions: Genial/Caring, Forceful/Exacting, Reclusive/Remote, and Ardent/Expressive. Being Genial/Caring was associated with life satisfaction. Among professional attributes, personal self‐experiences, and parallel dimensions of relationship with clients correlated strongly.ConclusionsAnalyses of > 10,000 psychotherapists revealed meaningful variations in personal self relevant to personal and professional life.
Effect of intrapersonal emotional competences on the relationship between attachment insecurity and severity of eating disorder symptoms in patients with restrictive anorexia
Authors: Nandrino Jean‐Louis , Dodin Vincent , Cottencin Olivier , Doba Karyn 
Published: November 2019

AbstractObjectiveAttachment insecurity and emotional competences (EC) form a key part of conceptual models of anorexia nervosa (AN). We explored the relationship between attachment dimensions and EC on the severity of eating disorders in patients diagnosed with restrictive AN.MethodSixty‐three female patients with restrictive AN and 63 healthy participants completed self‐report measures (eating symptoms, EC, attachment, depression, and anxiety).ResultPatients with restrictive AN used fewer adaptive and more maladaptive regulation strategies and showed low levels of intrapersonal EC. The partial least squares path modeling analysis showed that high levels of attachment anxiety or avoidance lead to a decrease in intrapersonal EC, which in turn contributes to greater severity of eating symptoms in anorexic patients.ConclusionsLower intrapersonal EC played an important mediating role in the effects of attachment insecurity on the severity of eating disorders. The joint use of therapeutic programs that target both EC and attachment processes constitutes a promising approach.
Emotional responding: Integration of multiple constructs and association with psychological health
Authors: Clifton Jessica , Seehuus Martin , Parent Justin , Pichler Emily , Fondacaro Karen 
Published: November 2019

AbstractObjectiveNumerous psychological constructs exist to describe different facets of emotional responding, but they have rarely been examined together. We empirically modeled the associations between four psychological constructs (mindfulness, emotional nonacceptance, experiential avoidance, and anxiety sensitivity) of individuals' responses to their affective experience, hypothesizing that a bifactor model would fit the data best.MethodWe used exploratory structural equation modeling, a novel latent variable modeling framework, to compare five measurement models of emotional responding in an online community sample (N = 307).ResultsA bifactor model including a general emotional responding factor had substantial factor loadings from nearly all items, with mixed results for specific factors. Exploratory analyses supported the significant association of avoidant emotional responding and psychopathology/well‐being.ConclusionThe general avoidant emotional responding factor appears to overlap most directly with experiential avoidance and may be a transtheoretical construct relevant to mental health.
GET.HAPPY2 – User perspectives on an internet‐based self‐management positive psychology intervention among persons with and without depression: Results from a retrospective survey
Authors: Görges Frauke , Oehler Caroline , von Hirschhausen Eckart , Hegerl Ulrich , Rummel‐Kluge Christine 
Published: November 2019

AbstractObjectivePrevious research suggests that online positive psychology interventions (PPI) are frequently used by individuals with symptoms of depression. We aimed to investigate differences in the way depressed and nondepressed users react to the content of an existing online PPI, originally designed for the general public.MethodIn a retrospective online survey, we assessed discontinuation parameters, aspects of satisfaction with the program, and negative reactions among users of an online PPI.ResultsBivariate and multivariate analyses showed that, overall, reactions between depressed and nondepressed individuals were similar. Differences were observed concerning reasons for using and for discontinuing the program, the perception of exercises, and negative reactions.ConclusionsAlthough satisfaction with the program was high, it did not seem to fully meet users’ expectations and might be more difficult to complete during episodes of depression. Implications of this study for the adaptation of online PPIs addressing depressed individuals are discussed.
Veterans’ perspectives on discussing moral injury in the context of evidence‐based psychotherapies for PTSD and other VA treatment
Authors: Borges Lauren M. , Bahraini Nazanin H. , Holliman Brooke Dorsey , Gissen Maura R. , Lawson W. Cole , Barnes Sean M. 
Published: November 2019

AbstractObjectiveWe examined Veterans’ perspectives on discussing moral injury in veterans affairs (VA) evidence‐based psychotherapies (EBPs) for posttraumatic stress disorder (PTSD) and other VA treatment.MethodsFourteen male warzone veterans (ages 25–74) who completed an EBP for PTSD within the past year participated in semistructured interviews related to discussing moral injury in VA treatment (e.g., EBPs for PTSD, chaplaincy). Qualitative interviews were evaluated using a thematic analysis.ResultsFour themes were identified; moral injury is often not identified or discussed during therapy, therapeutic relationships can promote or inhibit discussion of moral injury, treatment has limited impact on moral injury, and it is difficult to cope with moral injury even after treatment.ConclusionThe majority of Veterans interviewed identified moral injury persisting within a year of completing a PTSD EBP. These findings highlight the value of asking about, assessing, and treating moral injury in Veterans. Our results suggest the importance of developing specific moral injury interventions for warzone Veterans.
The relationship with the voices as a dialogical experience: The role of self‐focused attention and dissociation
Authors: Perona‐Garcelán Salvador , López‐Jiménez Ana M. , Bellido‐Zanin Gloria , Senín‐Calderón Cristina , Ruiz‐Veguilla Miguel , Rodríguez‐Testal Juan F. 
Published: November 2019

AbstractObjectiveThe purpose of this article was to study the relationship of self‐focused attention and dissociation with the dialogical relationship persons diagnosed with psychosis have with their voices.MethodThe DAIMON Scale was applied to 62 persons diagnosed with psychosis to measure the dialogical relationship with their voices, and the Cambridge depersonalization scale, the Tellegen absorption scale, and the self‐focused attention scale.ResultsThe results showed that the dialogical relationship with the voices was associated with high levels of self‐focused attention (private and public), depersonalization, and absorption. It was also found that absorption mediated significantly between public self‐focused attention and the dialogical relationship with the voices.ConclusionsThe role of dissociation and self‐focused attention in forming the dialogical relationship a person with psychosis has with the voices is discussed and approaches to treatment are suggested.
Combined enhancement effects of static magnetic field (SMF) and a yeast Candida tropicalis SYF‐1 on continuous treatment of Acid Red B by activated sludge under hypersaline conditions
Authors: Tan Liang , Mu Guangdi , Shao Yifan , Ning Shuxiang , Shi Shengnan 
Published: November 2019

AbstractBACKGROUNDLarge amounts of wastewater containing azo dyes and salts (e.g. from textile and dyeing processes) are produced each year, which need appropriate treatment before discharge. In the present study, combined enhancement effects of static magnetic field (SMF) and a halotolerant yeast Candida tropicalis SYF‐1 on continuous treatment of Acid Red B (ARB) under hypersaline conditions (containing 30 g L−1 NaCl) were estimated in a sequencing batch reactor (SBR). The dynamics of the microbial community was also analyzed.RESULTSRemoval percentages of ARB concentration, chemical oxygen demand (COD) and acute toxicity in the yeast–SMF co‐enhanced SBR (named as MSF‐SBR) were finally stabilized at about 98.0, 98.2 and 72.9% respectively, which were the highest among all SBRs. MSF‐SBR also possessed the highest biomass concentration (∼7462.1 mg L−1) and the best sludge settleability. Higher activities of nicotinamide adenine dinucleotide‐dependent dichlorophenol indophenol (NADH‐DCIP) reductase, lignin peroxidase (LiP) and manganese peroxidase (MnP) in MSF‐SBR were determined as responsible for higher degradation and detoxification efficiency. Bacterial genus Defluviimonas was putatively enriched in MSF‐SBR, which might be responsible for detoxification of aromatic intermediates. Candida was identified as the most dominant fungal genus in MSF‐SBR with a total relative abundance of 8.15%, which was responsible for effective dye degradation and detoxification.CONCLUSIONMSF‐SBR displayed higher and more stable treatment efficiency of ARB under hypersaline and continuous operation conditions. This study may provide a potentially applicable method for coping with purification of hypersaline industrial wastewaters. © 2019 Society of Chemical Industry
Taxonomic significance of cypsela morphology in tribe Cichoreae (Asteraceae) using light microscopy and scanning electron microscopy
Authors: Ayaz Sana , Ahmad Mushtaq , Zafar Muhammad , Ali Muhammad I. , Sultana Shazia , Mustafa Muhammad Raza Ul , Kilic Omer , Çobanoğlu Duygu N. , Demirpolat Azize , Ghani Abdul , Afza Rabia , Ahmad Khalid , Munir Mamoona , Kalsoom Nomana , Raza Jamil 
Published: November 2019

AbstractThe current study deals with the detailed morphology investigation of eight Cypsela species belonging to tribe Cichoreae. The different Cypsela types were described, explained, compared, and their taxonomic significance is discussed in detail. Light microscopy (LM) and scanning electron microscopy (SEM) have been used to highlight quantitative and qualitative characters of underestudied species. Cypsela exhibit great diversity in macro and micromorphological features such as shape, color, length, width, anticlinal and periclinal wall patterns, surface patterns, epicuticular projections. Majority of Cypsela species were brownish in color and their size ranges from 2.16 to 3.98 mm in length and 1.16 to 0.82 mm in breadth. A great diversity in Cypsela shapes like oblanceolate to obovate, obovoid to cylindrical, obvate, narrowly lanceolate were observed. Most of the platelets having epicuticular projections were observed. The surface pattern on the cypsela surface varied from rugose papillate, verrucose papillate, and striated. On the basis of considerable variations observed, the present study can assist as useful constraints at various taxonomic levels. The aim of the present study is to provide a comprehensive description of the Cypsela morphology and to determine the extent to which these micro morphological data can be used as a taxonomic character to delineate various taxa belonging to the tribe Cichoreae.