bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prevalence and risk factors of myopia in Han and Yugur older adults in Gansu, China: a cross-sectional study
Authors: Wang Xuqian , He Huijing , Wang Xuejiao , Shan Guangliang , Tao Zhiyan , Pan Li , Li Jun , Ren Xiaolan , Zhao Hongjun , Pan Zhouxian , Wang Meng , Zhong Yong , Ma Jin 
Published: May 2020

AbstractFew studies have investigated the prevalence of myopia in Northwest China. This cross-sectional study aimed to investigate the prevalence and associated factors of myopia and high myopia in adults aged 40–80 years in the Han and Yugur populations living in Gansu Province, Northwest China. A total of 3,845 participants were included. The overall age- and sex-adjusted prevalence of myopia (spherical equivalent (SE) < −0.5 D), high myopia (SE < −6.0 D) and hyperopia (SE > + 0.5 D) were 16.4%, 0.7% and 26.2% in Yugur participants, respectively, and 34.3%, 5.0% and 19.2% in Han participants, respectively. The prevalence of myopia and high myopia in Han participants was significantly higher than that in Yugur participants (bothP < 0.001). Yugur population, birth in rural areas, smoking history and outdoor work were found to be negatively associated with myopia. Higher education level and a family history of myopia were found to be positively associated with myopia in the study population. High myopia was negatively associated with Yugur population, aging, birth in rural areas and was positively associated with a family history of myopia. This study provided valuable information regarding the environmental risk factors of myopia and revealed an ethnic disparity in the prevalence of myopia in Gansu Province, Northwest China.
Vacancy-Hydrogen Interaction in Niobium during Low-Temperature Baking
Authors: Wenskat Marc , Čižek Jakub , Liedke Maciej Oskar , Butterling Maik , Bate Christopher , Haušild Petr , Hirschmann Eric , Wagner Andreas , Weise Hans 
Published: May 2020

AbstractA recently discovered modified low-temperature baking leads to reduced surface losses and an increase of the accelerating gradient of superconducting TESLA shape cavities. We will show that the dynamics of vacancy-hydrogen complexes at low-temperature baking lead to a suppression of lossy nanohydrides at 2 K and thus a significant enhancement of accelerator performance. Utilizing Doppler broadening Positron Annihilation Spectroscopy, Positron Annihilation Lifetime Spectroscopy and instrumented nanoindentation, samples made from European XFEL niobium sheets were investigated. We studied the evolution of vacancies in bulk samples and in the sub-surface region and their interaction with hydrogen at different temperature levels during in-situ and ex-situ annealing.
Establishment of an in vitro RNA polymerase transcription system: a new tool to study transcriptional activation in Borrelia burgdorferi
Authors: Boyle William K. , Hall Laura S. , Armstrong Anthony A. , Dulebohn Daniel P. , Samuels D. Scott , Gherardini Frank C. , Bourret Travis J. 
Published: May 2020

AbstractThe Lyme disease spirochete Borrelia burgdorferi exhibits dramatic changes in gene expression as it transits between its tick vector and vertebrate host. A major hurdle to understanding the mechanisms underlying gene regulation in B. burgdorferi has been the lack of a functional assay to test how gene regulatory proteins and sigma factors interact with RNA polymerase to direct transcription. To gain mechanistic insight into transcriptional control in B. burgdorferi, and address sigma factor function and specificity, we developed an in vitro transcription assay using the B. burgdorferi RNA polymerase holoenzyme. We established reaction conditions for maximal RNA polymerase activity by optimizing pH, temperature, and the requirement for divalent metals. Using this assay system, we analyzed the promoter specificity of the housekeeping sigma factor RpoD to promoters encoding previously identified RpoD consensus sequences in B. burgdorferi. Collectively, this study established an in vitro transcription assay that revealed RpoD-dependent promoter selectivity by RNA polymerase and the requirement of specific metal cofactors for maximal RNA polymerase activity. The establishment of this functional assay will facilitate molecular and biochemical studies on how gene regulatory proteins and sigma factors exert control of gene expression in B. burgdorferi required for the completion of its enzootic cycle.
A Micro-CT and Synchrotron Imaging Study of the Human Endolymphatic Duct with Special Reference to Endolymph Outflow and Meniere’s Disease
Authors: Nordström Charlotta Kämpfe , Li Hao , Ladak Hanif M. , Agrawal Sumit , Rask-Andersen Helge 
Published: May 2020

AbstractMeniere’s disease remains enigmatic, and has no treatment with sufficient evidence. The characteristic histopathological finding is endolymphatic hydrops, suggesting either an overproduction or decreased reabsorption of endolymph in the human inner ear. This study presents the first analysis of the vascular plexus around the human endolymphatic duct using micro computed tomography and coherent synchrotron radiation with phase contrast imaging. Using a software program, data were processed by volume-rendering with scalar opacity mapping to create transparent three-dimensional reconstructions. A rich vascular plexus was discovered around the endolymphatic duct that drained into collecting channels, linked to the vestibular venous outflow system. This network is believed to make up the principal route for endolymph outflow, and its associated malfunction may result in endolymphatic hydrops and Meniere’s disease.
Decreased temperature variance associated with biotic composition enhances coastal shrub encroachment
Authors: Wood Lauren K. , Hays Spencer , Zinnert Julie C. 
Published: May 2020

AbstractRegime shift from grasslands to shrub-dominated landscapes occur worldwide driven by altered land-use and climate change, affecting landscape function, biodiversity, and productivity. Warming winter temperatures are a main driver of expansion of the native, evergreen shrub, Morella cerifera, in coastal landscapes. Shrub establishment in these habitats alters microclimate, but little is known about seasonal differences and microclimate variance. We assessed influence of shrubs on microclimate variance, community composition, and community physiological functioning across three vegetation zones: grass, transitional, and shrub in a coastal grassland. Using a novel application of a time-series analysis, we interpret microclimatic variance modification and elucidate mechanisms of shrub encroachment at the Virginia Coast Reserve, Long-Term Ecological Research site. As shrub thickets form, diversity is reduced with little grass/forb cover, while transpiration and annual productivity increase. Shrub thickets significantly reduced temperature variance with a positive influence of one day on the next in maximum air, minimum air, and maximum ground temperature. We also show that microclimatic temperature moderation reduces summer extreme temperatures in transition areas, even before coalescence into full thickets. Encroachment of Morella cerifera on the Virginia barrier islands is driven by reduced local exposure to cold temperatures and enhanced by abiotic microclimatic modification and biotic physiological functioning. This shift in plant community composition from grassland to shrub thicket alters the role of barrier islands in productivity and can have impacts on the natural resilience of the islands.
Drosophila taste neurons as an agonist-screening platform for P2X receptors
Authors: Grimes Leanne , Griffiths Julia , Pasqualetto Gaia , Brancale Andrea , Kemp Paul J. , Young Mark T. , van der Goes van Naters Wynand 
Published: May 2020

AbstractThe P2X receptor family of ATP-gated cation channels are attractive drug targets for pain and inflammatory disease, but no subtype-selective agonists, and few partially selective agonists have been described to date. As proof-of-concept for the discovery of novel P2X receptor agonists, here we demonstrate the use of Drosophila taste neurons heterologously expressing rat P2X2 receptors as a screening platform. We demonstrate that wild-type rat P2X2 expressed in Drosophila is fully functional (ATP EC50 8.7 µM), and that screening of small (2 µl) volumes of a library of 80 adenosine nucleotide analogues is rapid and straightforward. We have determined agonist potency and specificity profiles for rat P2X2 receptors; triphosphate-bearing analogues display broad activity, tolerating a number of substitutions, and diphosphate and monophosphate analogues display very little activity. While several ATP analogues gave responses of similar magnitude to ATP, including the previously identified agonists ATPγS and ATPαS, we were also able to identify a novel agonist, the synthetic analogue 2-fluoro-ATP, and to confirm its agonist activity on rat P2X2 receptors expressed in human cells. These data validate our Drosophila platform as a useful tool for the analysis of agonist structure-activity relationships, and for the screening and discovery of novel P2X receptor agonists.
Revealing the main factors and two-way interactions contributing to food discolouration caused by iron-catechol complexation
Authors: Bijlsma Judith , de Bruijn Wouter J. C. , Hageman Jos A. , Goos Peter , Velikov Krassimir P. , Vincken Jean-Paul 
Published: May 2020

AbstractFortification of food with iron is considered to be an effective approach to counter the global health problem caused by iron deficiency. However, reactivity of iron with the catechol moiety of food phenolics leads to discolouration and impairs bioavailability. In this study, we investigated the interplay between intrinsic and extrinsic factors on food discolouration caused by iron-catechol complexation. To this end, a three-level fractional factorial design was implemented. Absorbance spectra were analysed using statistical methods, including PCA, HCA, and ANOVA. Furthermore, a direct link between absorbance spectra and stoichiometry of the iron-catechol complexes was confirmed by ESI-Q-TOF-MS. All statistical methods confirm that the main effects affecting discolouration were type of iron salt, pH, and temperature. Additionally, several two-way interactions, such as type of iron salt × pH, pH × temperature, and type of iron salt × concentration significantly affected iron-catechol complexation. Our findings provide insight into iron-phenolic complexation-mediated discolouration, and facilitate the design of iron-fortified foods.
Sulfonamides-induced oxidative stress in freshwater microalga Chlorella vulgaris: Evaluation of growth, photosynthesis, antioxidants, ultrastructure, and nucleic acids
Authors: Chen Shan , Wang Liqing , Feng Wenbo , Yuan Mingzhe , Li Jiayuan , Xu Houtao , Zheng Xiaoyan , Zhang Wei 
Published: May 2020

AbstractSulfadiazine (SD), sulfamerazine (SM1), and sulfamethazine (SM2) are widely used and disorderly discharged into surface water, causing contamination of lakes and rivers. However, microalgae are regard as a potential resource to alleviate and degrade antibiotic pollution. The physiological changes of Chlorella vulgaris in the presence of three sulfonamides (SAs) with varying numbers of –CH3 groups and its SA-removal efficiency were investigated following a 7-day exposure experiment. Our results showed that the growth inhibitory effect of SD (7.9–22.6%), SM1 (7.2–45.9%), and SM2 (10.3–44%) resulted in increased proteins and decreased soluble sugars. Oxidative stress caused an increase in superoxide dismutase and glutathione reductase levels but decreased catalase level. The antioxidant responses were insufficient to cope-up with reactive oxygen species (hydrogen peroxide and superoxide anion) levels and prevent oxidative damage (malondialdehyde level). The ultrastructure and DNA of SA-treated algal cells were affected, as evident from the considerable changes in the cell wall, chloroplast, and mitochondrion, and DNA migration. C. vulgaris-mediated was able to remove up to 29% of SD, 16% of SM1, and 15% of SM2. Our results suggest that certain concentrations of specific antibiotics may induce algal growth, and algal-mediated biodegradation process can accelerate the removal of antibiotic contamination.
Heat vortex in hydrodynamic phonon transport of two-dimensional materials
Authors: Shang Man-Yu , Zhang Chuang , Guo Zhaoli , Lü Jing-Tao 
Published: May 2020

AbstractWe study hydrodynamic phonon heat transport in two-dimensional (2D) materials. Starting from the Peierls-Boltzmann equation with the Callaway model approximation, we derive a 2D Guyer-Krumhansl-like equation describing hydrodynamic phonon transport, taking into account the quadratic dispersion of flexural phonons. In addition to Poiseuille flow, second sound propagation, the equation predicts heat current vortices and negative non-local thermal conductance in 2D materials, which are common in classical fluids but have not yet been considered in phonon transport. Our results also illustrate the universal transport behaviors of hydrodynamics, independent of the type of quasi-particles and their microscopic interactions.
A novel third mesh-like myometrial layer connects the longitudinal and circular muscle fibers -A potential stratum to coordinate uterine contractions-
Authors: Kagami Kyosuke , Ono Masanori , Iizuka Takashi , Matsumoto Takeo , Hosono Takashi , Sekizuka-Kagami Naomi , Shinmyo Yohei , Kawasaki Hiroshi , Fujiwara Hiroshi 
Published: May 2020

AbstractPeriodic myometrial contraction is one of the important uterine functions to achieve embryo implantation and parturition. Although it is well-known that the mammalian myometrium is composed of longitudinal (outer) and circular (inner) layers, the precise mechanisms that coordinate both muscular contractions to produce peristaltic movements remain unclear. Recently, by treatment with our modified Clear Unobstructed Brain Imaging Cocktails and Computational analysis (CUBIC) tissue-clearing method, we obtained well-contrasted three-dimensional images of the transparent murine ovary using enhanced green fluorescent protein (EGFP) transgenic mice and light-sheet microscopy. Consequently, to investigate accurate anatomical connections between outer and inner myometrial fibers, we observed whole structures of the myometrium using a transparent murine uterus. By this method, we identified a novel muscle layer, a middle layer of the myometrium, which anatomically connects the conventional outer longitudinal and inner circular muscles. This new layer was visualized as a mesh-like structure and this structure was observed throughout the whole uterus from proximal to distal sites. In this area, CD31-positive vessels were abundantly localized around the mesh-like muscle fibers. In addition, CD34-positive uterine telocytes and tubulin β-3-positive nerve fibers were closely located in this middle layer. These findings indicate the presence of a novel mesh-like stratum that connects longitudinal and circular muscle layers, and suggest its coordinating role in myometrial contractions.
Transformer winding type recognition based on FRA data and a support vector machine model
Authors: Mao Xiaozhou , Wang Zhongdong , Crossley Peter , Jarman Paul , Fieldsend‐Roxborough Andrew , Wilson Gordon 
Published: May 2020

Frequency response analysis (FRA) is regarded as the most effective technique to detect mechanical faults of transformers. Over the years, FRA measurement data have been collected by utilities into transformer asset databases. The characteristic of FRA data is fundamentally determined by the transformer's equivalent electrical circuit, which consists of inductance and capacitance parameters that are windings' design and structure dependent. Different winding types tend to have different FRA characteristics, and a transformer's design information such as winding type, dimension etc. is often not known to the utility but critically important for asset management. This study reviews the state‐of‐the‐art transformer FRA databases and application of machine learning techniques in this field, and proposes to apply a support vector machine (SVM) model onto the FRA data to identify the winding type. The SVM model is first trained by FRA traces of transformers with known winding types, and after testing, the SVM model is then applied to FRA traces with unknown winding information. A set of data from the UK's National Grid FRA database, was used to demonstrate and verify the SVM model. All transformers used in this study are 400/275/13 kV transmission transformers, which were designed using four different winding types, namely multiple layer, plain disc, interleaved disc and single helical windings. The proposed method can successfully identify the correct winding type.
Dielectric characterisation of epoxy nanocomposite with barium titanate fillers
Authors: Zafar Romana , Gupta Nandini 
Published: May 2020

High permittivity materials are currently in use for mitigation of electrical stress in high‐voltage apparatus and energy storage systems. In this work, epoxy‐based high permittivity nanocomposites with Barium titanate (BaTiO3) nanofillers are considered, for the purpose of stress mitigation. Uniform dispersion of the fillers in the polymer up to 10% by volume is achieved. Apart from the use of as‐received fillers, the effect of using surface‐functionalised nanoparticles (with 3‐glycidoxypropyltrimethoxy‐silane) before use is also investigated. The nanocomposite is characterised in terms of its complex permittivity, DC conductivity, short‐term AC breakdown strength and space charge accumulation, to gauge its suitability for use in high‐voltage insulation. Complex permittivity is measured using broadband dielectric spectroscopy over a broad frequency range of 1 mHz to 1 MHz. DC conductivity is studied from polarisation–depolarisation current measurements. Short‐term AC breakdown strength tests are performed at power frequency (50 Hz). Space charge density along the sample thickness is obtained using pulsed electro‐acoustic technique. A computational case‐study is presented to show the feasibility of using the high permittivity nanocomposite for electric stress control in high‐voltage equipment (viz., at mounting flanges of 69 kV bushings).
Prognostic Impact of Adverse Pathologic Features in Sinonasal Squamous Cell Carcinoma
Authors: Parikh Anuraag S. , Fuller Jennifer C. , Lehmann Ashton E. , Goyal Neerav , Gray Stacey T. , Lin Derrick T. 
Published: May 2020

Abstract
Objective This study aimed to describe the impact of adverse clinical and pathologic features in sinonasal squamous cell carcinoma (SCC).
Design This study is designed with retrospective chart review.
Setting The present study is conducted at a tertiary care institution.
Participants All patients treated surgically for sinonasal SCC at our tertiary care institution between January 2006 and December 2013.
Main Outcome Measures Overall survival (OS) and disease free survival (DFS) are the final measurement of this study.
Results Forty-eight patients were identified. Mean age at surgery was 65.8 years, and mean follow-up time was 40.7 months. Eighteen patients (38%) had T1–T3 disease, while 30 patients (63%) had T4 disease. Seven patients (8.3%) had nodal disease at presentation. At 2, 5, and 10 years, OS was 71, 54, and 48%, respectively, while DFS was 64, 51, and 45%, respectively. Twelve patients (25%) experienced local recurrences with mean time to recurrence of 15.3 months. Twenty-five patients (52%) had positive margins, 24 (50%) had high-grade tumors, 18 (38%) had perineural invasion (PNI), and 15 (31%) had lymphovascular invasion (LVI). In the univariate analysis, T4 disease (risk ratio [RR] = 2.7) and high grade (RR = 2.4) had a significant association with DFS. In the multivariate analysis, high grade (RR = 4.0 and 4.5) and LVI (RR = 4.1 and 4.7) had a significant association with OS and DFS.
Conclusion Our single-institution experience of 48 patients suggests that high grade and LVI are independently associated with survival outcomes in sinonasal SCC, while PNI and microscopically positive margins do not have a significant impact.
Phage therapy as a potential solution in the fight against AMR: obstacles and possible futures
Authors: Brives Charlotte , Pourraz Jessica 
Published: May 2020

AbstractPhage therapy, the use of bacteriophage viruses to treat bacterial infections, has existed for more than a hundred years. However, the practice is struggling to develop, despite growing support over the past 15 years from researchers and doctors, who see it as a promising therapy in the context of the rise of antimicrobial resistance (AMR). While the reasons for these developmental difficulties are complex, in this article we wish to address the effects of pharmaceutical regulations on phage therapy. By showing how phages are assimilated to an umpteenth antibiotic in legal texts, but also in certain medical practices, this article proposes to analyze the consequences of such regulatory categorization both for their production and the logistics of administration of proof of their efficacy in randomized controlled trials (RCTs), as well as the underlying concepts of infection and treatment. This paper follows Chandler’s work on the concept of antibiotics as infrastructure and its inversion presented by antimicrobial resistance. Phages as living, dynamic, evolving, and specific entities, do not lend themselves easily to current categories, norms, and development models. In this sense, they act as disruptors, revealing the limitations imposed by the existing infrastructure. More precisely here, and to continue Chandler’s initial thought process, this paper aims to show that antibiotics also form a kind of epistemological infrastructure, which acts as a powerful inhibitor to the development of phage therapy. In this sense antibiotics prevent the development of solutions to the problem they contribute to create. But the difficulties phage therapy faces, as highlighted in this article, can be interpreted as entry points for thinking of another medicine and imagining other possible futures. This analysis is based on a 3-year fieldwork study (2016–2019) in Europe (France, Belgium, and Switzerland), during which we conducted semi-directed interviews with various phage therapy stakeholders (physicians, researchers, pharmacists, regulators, patients, and patient associations), participatory observation in labs and observations during symposia and workshops on phages and phage therapy.
Ediacaran reorganization of the marine phosphorus cycle
Authors: Laakso Thomas A. , Sperling Erik A. , Johnston David T. , Knoll Andrew H. 
Published: May 2020

SignificanceThe evolution of macroscopic animals in the latest Proterozoic Eon is associated with many changes in the geochemical environment, but the sequence of cause and effect remains a topic of intense research and debate. In this study, we use two apparently paradoxical observations—that massively phosphorus-rich rocks first appear at this time, and that the median P content of rocks does not change—to argue for a change in internal marine P cycling associated with rising sulfate levels. We argue that this change was self-sustaining, setting in motion a cascade of biogeochemical transformations that led to conditions favorable for major ecological and evolutionary change.