bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mouse γ-Synuclein Promoter-Mediated Gene Expression and Editing in Mammalian Retinal Ganglion Cells
Authors: Wang Qizhao , Zhuang Pei , Huang Haoliang , Li Liang , Liu Liang , Webber Hannah C. , Dalal Roopa , Siew Leonard , Fligor Clarisse M. , Chang Kun-Che , Nahmou Michael , Kreymerman Alexander , Sun Yang , Meyer Jason S. , Goldberg Jeffrey Louis , Hu Yang 
Published: April 2020

Optic neuropathies are a group of optic nerve (ON) diseases caused by various insults including glaucoma, inflammation, ischemia, trauma, and genetic deficits, which are characterized by retinal ganglion cell (RGC) death and ON degeneration. An increasing number of genes involved in RGC intrinsic signaling have been found to be promising neural repair targets that can potentially be modulated directly by gene therapy, if we can achieve RGC specific gene targeting. To address this challenge, we first used adeno-associated virus (AAV)-mediated gene transfer to perform a low-throughputin vivoscreening in both male and female mouse eyes and identified the mouse γ-synuclein (mSncg) promoter, which specifically and potently sustained transgene expression in mouse RGCs and also works in human RGCs. We further demonstrated that gene therapy that combines AAV-mSncg promoter with clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 gene editing can knock down pro-degenerative genes in RGCs and provide effective neuroprotection in optic neuropathies.SIGNIFICANCE STATEMENTHere, we present an RGC-specific promoter, mouse γ-synuclein (mSncg) promoter, and perform extensive characterization and proof-of-concept studies of mSncg promoter-mediated gene expression and clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 gene editing in RGCsin vivo. To our knowledge, this is the first report demonstratingin vivoneuroprotection of injured RGCs and optic nerve (ON) by AAV-mediated CRISPR/Cas9 inhibition of genes that are critical for neurodegeneration. It represents a powerful tool to achieve RGC-specific gene modulation, and also opens up a promising gene therapy strategy for optic neuropathies, the most common form of eye diseases that cause irreversible blindness.
Diksriminasi terhadap Perempuan Dalam Novel Sunyi di Dada Sumirah Karya Artie Ahmad
Authors: Rahayu Umi , Andalas Maharani Intan 
Published: April 2020

Penelitian berjudul ”Diskriminasi Terhadap Perempuan dalam Novel Sunyi di Dada Sumirah karya Artie Ahmad” bertujuan untuk mengetahui diskriminasi terhadap perempuan dalam novel tersebut. Dalam penelitian ini digunakan teori kritik sastra feminis dengan metode penelitian kualitatif. Hasil penelitian ini adalah ditemukannya diskriminasi terhadap perempuan dalam novel Sunyi di Dada Sumirah yakni stereotip, marginalisasi, dan kekerasan.
The study entitled "Women Discrimination in the Novel Sunyi di Dada Sumirah by Artie Ahmad" aims to find out women discrimination in the novel. In this study feminist literary criticism theory is used with qualitative research methods. The results of this study are the discovery of women discrimination in the novel Sunyi di Dada Sumirah namely stereotypes, marginalization, and violence.
Bersama Melawan Virus Covid 19 di Indonesia
Authors: Syafrida Syafrida , Hartati Ralang 
Published: April 2020

AbstractCovid-19 hit many countries in the world including Indonesia. The Covid-19 outbreak is not only a national problem in a country, but already a global problem. Covid-19 originated in the Wuhan area of China. The spread of Covid-19 is so fast and deadly, transmission through physical contact is transmitted through the mouth, eyes and nose. Covid-19 has an impact on social life and the weakening of the people's economy. The issue of how the impact of the Covid-19 outbreak on public services and efforts to overcome the Covid-19 outbreak. The juridical normative research method is supported by empirical research using secondary data in the form of books, legislation and the internet. Type of qualitative research. The results of the study concluded that the impact of the Covid-19 outbreak caused social problems to weaken the economy of the community and the state. To prevent the spread of the Covid-19 outbreak, it needed the cooperation of governments, communities, religious leaders to help one another help, help one another and remind one another to deepen Covid-19.Keywords: Fight, Covid-19, Indonesia AbstrakCovid-19 melanda banyak Negara di dunia termasuk Indonesia. Wabah Covid-19 tidak hanya merupakan masalah nasional dalam suatu Negara, tapi sudah merupakan masalah global. Covid-19 berawal muncul dari daerah Wuhan Cina. Penyebaran Covid -19 yang begitu cepat dan mematikan,, penularannya melalui kontak fisik ditularkan melalui mulut, mata dan hidung. Covid-19 berdampak kepada kehidupan sosial dan melemahnya ekonomi masyarakat. Permasalahan bagaimana dampak wabah Covid-19 terhadap pelayanan publik dan upaya penanggulangan wabah Covid-19. Metode penelitian bersifat yuridis normatif yang didukung penelitian empiris menggunakan data sekunder berupa buku-buku, peraturan perundangan-undangan dan internet. Jenis penelitian kualitatif. Hasil penelitian disimpulkan dampak wabah Covid-19 menimbulkan masalah sosial pelemahan ekonomi masyarakat dan negara, Untuk mencegah penyebaran wabah Covid- 19 diperlukan kerja sama pemerintah, masyarakat, tokoh agama untuk saling bantu membatu, bahu membahu dan mengingatkan satu sama lainnya bersama melawan Covid-19. Kata kunci: Melawan, Covid-19, Indonesia.
The estimation scale of the daily pedometry of senior students
Authors: Kolokoltsev Mikhail M. , Romanova Elena V. , Jagiello Wladyslaw , Yermakova Tetiana S. 
Published: April 2020

Background and Study Aim: To create the estimation scale of the daily pedometry of senior students with different levels of motor activity. To determine the dependence of students' life quality on the volume of step locomotions. Material and methods. The students of Irkutsk National Research Technical University (Irkutsk, Russia) (n = 235: females - n = 78, males - n = 157: 19-20 years old) participated in the study. The daily pedometry of students for 7 days was studied. The number of daily steps was recorded by Simple Design Ltd application for smartphones. The sigma deviation method was used to develop an individual daily pedometry estimation scale. The daily pedometry scale was developed based on the distribution of this characteristic by 5 sigma classes ("low", "below average", "average", "above average", "high"). Students' life quality was studied using a Russian-language version of SF-36 (Short Form Health Survey). The survey presents the characteristics of physical (PH), mental (MH) and general (GH) health. The answers were estimated in points (0-100). Results. Most students have an "average" level of step locomotions. 10% of males and about 8% of females have "above average" or "high" pedometry level. This group of students is actively engaged in sports activities. 4.8% of males and 7.5% of females have a "low" pedometry level. In this group of students, motor activity is limited only by locomotions related to educational and household activities. Students with "high" and "above average" pedometry levels have the highest values of life quality indicators. Such students are in a safe zone of non-communicable diseases. The “low” level of daily step locomotions is considered as a predictor of insufficient physical activity, low general and mental health of students. Conclusions. The sigma deviation method allows distributing any population of people by the number of daily locomotions into five sigma classes. The level of daily step locomotions can be considered as a marker of physical activity, general and mental health. This approach makes it possible to refer a person to a safe health zone or a risk group of non-communicable diseases. The results of the study can be used in the recommendations for improving the students’ life quality. It is also recommended to use our recommendations to increase students' self-motor activity during their university studies.
The ratio of shear to elastic modulus of in-plane loaded masonry
Authors: Wilding Bastian Valentin , Godio Michele , Beyer Katrin 
Published: April 2020

AbstractWhen designing unreinforced masonry buildings, the wall stiffness and, consequently, the masonry elastic and shear modulusEandGare essential parameters. Current codes provide empirical estimates of the masonry elastic modulus and a ratio between the shear and elastic modulus,G/E. This ratio, commonly taken as 0.4, is not based on scientific evidence and there appears to be no consensus concerning its value and influencing parameters, meaning that current code standards might not accurately portray the shear deformations of masonry elements. To give the choice of theG/Eratio a theoretical foundation, this paper presents closed-form expressions for theG/Eratio of running-bond masonry that capture the effects of finite joint thickness, finite wall thickness and orthotropic block properties. Based on the geometry of blocks and joints as well as their elastic parameters, a validation of the developed expression using 3D finite element analyses shows good performance. For modern masonry typologies with hollow clay bricks, aG/Eratio of 0.20–0.25 is obtained. For historical masonry typologies, such as dry stacked or mortared stone masonry, as well as solid clay brick masonry, ratios between 0.30 and 0.40 are computed.
PENGARUH KEMAMPUAN DAN MOTIVASI KERJA TERHADAP KINERJA KARYAWAN BAGIAN PRODUKSI PT. SENTOSA HASTAREKSA
Authors: Sari Whidya Udaya , Sumiyati Sumiyati , Utama Dian Herdiana 
Published: April 2020

Persaingan dunia usaha yang semakin kompetitif, menuntut perusahaan untuk memiliki kinerja yang tinggi. Peningkatan kinerja sumber daya manusia (SDM) merupakan hal yang sangat penting dalam memperbaiki keberhasilan perusahaan, sehingga perlu diupayakan secara terus menerus dan berkesinambungan dalam menghadapi dunia global terutama pada industri ekspor impor di Indonesia. Kemampuan dan motivasi kerja dijadikan suatu cara untuk meningkatkan kinerja karyawan di bagian produksi PT. Sentosa Hastareksa. Penelitian ini bertujuan untuk mengetahui gambaran kemampuan, motivasi kerja terhadap kinerja karyawan, serta pengaruh kemampuan dan motivasi kerja terhadap kinerja karyawan bagian produksi PT. Sentosa Hastareksa. Objek penelitian ini adalah karyawan produksi PT. Sentosa Hastareksa. Variabel bebas dalam penelitian ini adalah kemampuan dan motivasi kerja, serta variabel terikat yaitu kinerja karyawan dengan jenis penelitian yang digunakan adalah deskriptif, verifikatif, dan metode yang digunakan adalah explanatory survey dengan teknik sampel simple random sampling dengan jumlah sampel 141 responden. Teknik analisa data yang digunakan adalah regresi liniear berganda dengan alat bantu software komputer SPSS 22.0 for windows. Hasil yang diperoleh dalam penelitian menyatakan bahwa kemampuan dan motivasi kerja berpengaruh terhadap kinerja karyawan. Dengan tingkat pengaruh kemampuan dan motivasi kerja terhadap kinerja karyawan kuat. Dari hasil penelitian terhadap pengujian hipotesis dapat diketahui bahwa kemampuan dan motivasi kerja memiliki pengaruh yang positif terhadap kinerja karyawan Kata Kunci: Kemampuan, Motivasi Kerja, Kinerja Karyawan.
GAMBARAN PROGRAM PROMOSI JABATAN, PENGHARGAAN DAN KEPUASAN KERJA KARYAWAN PT. GUNANUSA UTAMA FABRICATORS SERANG
Authors: Sari Dwi Ervita , Senen Syamsul Hadi , Razati Girang 
Published: April 2020

Purpose - The purpose of this study is to see the description of promotion program, reward and job satsfaction. Design/methodology/approachh - The design of this study was cross sectional method. This research uses descriptive approach with explanatory survey method. A total of 90 respondents were selected using probability sampling. A questionnaire was used as a research instrument to collect data from respondents. Analytical technique used is descriptive technique by using frequency distribution.Findings - Based on the results of the research using descriptive analysis, the results obtained result that promotion of good enough position with score 7396 acquisition equal to 73,37%, amount in category enough according to score 12118, obtained percentage equal to 73,98%. and job satisfaction in high enough category with score 12769 Earnings equal to 75,06%.Originality/value - This study provides a basis for understanding issues of promotion program, reward and job satsfaction. The difference of this study with the previous research is located on the object which is the company in the contuction industry that is PT. Gunanusa Utama Fabricators Serang, with the variables studied namely promotion program, reward and job satsfaction, and using different theory or reference from previous researcher.
Childhood autism associated with neurological manifestations and corpus callosum hypoplasia: literature review and clinical cases
Authors: Komissarova O. A. , Milovanova O. A. , Avakyan G. G. , Bugriy S. V. 
Published: April 2020

Aim. To consolidate literature data and to demonstrate rare hereditary neurogenetic syndromes with various neuropsychiatric manifestations and a corpus callosum (CC) structurally reduced in size.Material and methods. Full text data from scholarly journals were used for the review. Mental and neurological disorders were studied in two examined patients with neurogenetic syndromes. A brain MRI showed that it was accompanied with CC hypoplasia. We conducted comprehensive analysis of anamnestic data as well as medical genetic, neurological, psychopathological, pathopsychological, laboratory, and instrumental examinations.Results. The presented observations testified to a burdened obstetric history, dysfunctional ante-, intra-, and postnatal periods. The first patient with the Wolf-Hirschhorn syndrome was diagnosed with atypical autism associated with severe mental retardation, lack of verbal means of communication, motor stereotypes, structural focal epilepsy, cerebral palsy (CP), severe systemic speech underdevelopment; the second patient with the Prader-Willi syndrome was diagnosed with organic autism associated with mild mental retardation, impaired social adaptation in combination with stereotyped actions, impaired visual-spatial coordination, and cerebral palsy. In the first case, MRI showed posterior CC hypoplasia; in the second case, MRI showed hypoplasia of the CC isthmus.Conclusion. The level of cognitive deficit was to some extent a value associated with the thickness of the posterior CC. Apparently, a higher degree of myelination of nerve fibers contributes to a higher rate of transmission of nerve impulses along nerve fibers stimulating neurons. The results can be considered preliminary; a larger study is needed.
What Is Our “Epistemic Responsibility”? Reflections on Social Work’s Orientation in Yoga Therapy Research
Authors: Larocque Emmanuelle , Moreau Nicolas 
Published: April 2020

Abstract
The purpose of this article is to explore how the social work discipline could provide a complementary lens through which yoga therapy can be analyzed and evaluated by engaging in knowledge-creation practices and procedures that prioritize the “epistemic responsibility” described by philosopher Lorraine Code. More specifically, by seeking to strategically include often-subjugated types of knowledge and by focusing on redistributing epistemic power to agents that typically have been excluded from epistemic participation in contemporary yoga therapy research, the social work discipline, with its strong commitment to social justice, has the potential to contribute to filling an important gap in scientific literature. We begin by presenting the relevance of the social work perspective in relation to the field of yoga therapy. We next offer a reserved critical analysis of the dominant technical knowledge base that currently informs yoga therapy practice. This analysis highlights the social parameters that may be rendered invisible or left aside when adopting a positivist epistemological lens and justifies how the conceptual apparatus of epistemic responsibility serves as a potential platform for rethinking social work’s position and future contributions to the field of yoga therapy. Finally, we mobilize the concept of cultural appropriation to illustrate how striving for epistemic responsibility provides an entry point for addressing the multilevel, complex social processes embedded in yoga therapy practice and research while aiming to capture the many voices—and hence the various truths—implicated in a democratic, reflexive, and inclusive research process.
The effectiveness of the method of external counterpulsation in patients with chronic stable angina
Authors: Alakbarov R H 
Published: April 2020

Aim. To assess the effectiveness of the complex treatment of patients with stable angina pectoris using the method of external counterpulsation (ECP).
Methods. 92 patients with chronic stable angina were included in the comparative analysis, which divided into 2 groups: 57 patients received one course of treatment using the ECP method (main group), 35 patients received only drug treatment (control group). Before and after the therapy, a general clinical examination (including determining the functional class of angina pectoris and the need to use nitroglycerin preparations), coronary angiography, echocardiography, exercise stress test (stress ECG), and quality assessment using the Seattle Angina Questionnaire (SAQ) was performed.
Results. The decrease in the average functional class of angina was more significant in the main group compare to the control group from 2.280.73 to 0.930.80 (p 0.05) versus from 2.340.73 to 1.830.71 (p 0.05). A decrease of at least 1 functional class more often occurred in the main group 78.9% of patients versus 57.1% (p=0.0258). The use of nitroglycerin sharply decreased after treatment in the group, that use the ECP method (by 51.6%; p=0.002), whereas in the control group, despite a slight decrease (by 22.7%), the changes did not reach statistical significance (p=0.0736). Both groups showed similar dynamics of changes in echocardiography. The differences were obtained only for the dynamics of the ejection fraction of the left ventricle (LVEF), which was greater in the main group an increase of 4.695.56% versus 1.755.15% (p=0.0448). The dynamics of all indicators of exercise stress test significantly differ between groups, and if for the main group it is positive, in the control group it is negative. Some indicators of quality of life change insignificant and similar for both groups (for example, on the scale for limiting physical exertion), and on some scales (stability scales for attacks and the frequency of angina attacks), the improvement in the main group is much more significant in comparing with the control group: 3037 points versus 1213 points, respectively (p 0.0001). The overall quality of life assessment score improved more in the group, that use the ECP method: +96.1040.73 versus +45.3135.06 the control group (p 0.0001).
Conclusion. The method of external counterpulsation (ECP) can be used in clinical practice as part of a comprehensive treatment of patients with stable angina pectoris; it can significantly reduce the class of angina pectoris and the need to use nitroglycerin preparations, improve a number of indicators of echocardiography, electrocardiography with exercise and quality of life indicators.
Forecast‐Cartographic Modeling of Pasture Production of the Volgograd Region Based on Remote Sensing
Authors: Shinkarenko S. S. , Kosheleva O. Yu. , Solodovnikov D. A. 
Published: April 2020

Aim. The work is devoted to identifying the productivity of pasture landscapes in the Volgograd region. The aim was to determine the direction of trends and the values of the coefficients of proportionality which would permit the definition of areas where the productivity of natural zonal vegetation has increased or decreased from 2000 until today. Material and Methods. Pasture productivity assessment is based on the analysis of the NDVI vegetation index, which is widely used in such studies. For analysis, specific pasture areas were identified in accordance with Global Land Cover, divided into egular grids and given overlays corresponding with the boundaries of municipalities and landscapes. Results. The largest areas of natural zonal pastures are located in the Trans‐Volga region and on the sandy massifs of the Don River valley. About 60% of pasture land has an average weighted average long‐term NDVI value from 0.3 to 0.4, and approximately a quarter – from 0.4 to 0.5. In most parts of the region there are negative NDVI trends. The highest rate of degradation is noted in the Trans‐Volga region. This is associated with larger pasturing loads than in the rest of the region, as well as with the regular occurrence of steppe fires. Conclusion. In summation: the productivity trends of zonal pastures in the Volgograd region have been determined, as have areas with different NDVI directions and dynamics. The application of these results in practice should make it possible to predict pasture productivity in various municipal districts and landscapes of the region, and thus assist in the regulation of pasture loads and the mitigation of risks of vegetation degradation.
Use of Oxidative Stress (MDA) Markers and Cytogenetic Markers in the Ecological‐Genetic Monitoring of the Northern Caspian Sea
Authors: Kuzina T. V. , Kuzin A. V. 
Published: April 2020

Aim. Assessment and subsequent long‐term dynamic observation of possible negative genetic consequences of the effect of pollutants on certain units of metabolism are important tasks in ecological‐genetic monitoring. Cytogenetic and biochemical biomarkers are used in biomonitoring studies to analyze the genotoxicity of aquatic pollutants. The purpose of the work was to analyse the use of markers of oxidative stress and cytogenetic disorders in goby fish caught at shallow and deep‐water stations of the Northern Caspian Sea in the ecological‐genetic monitoring system. Material and Methods. The study was undertaken on 227 specimens of goby fish by cytogenetic and biochemical analysis. Results. The correlation dependence between erythrocytes with micronuclei and erythrocytes with the quantity of damaged nuclei summarized as R= ‐0.83 (p˂0.05) was shown. The results of correlation analysis between oxidative stress indices and the number of destructive changes in erythrocyte nucleus are presented. Our analysis thus leads us to the conclusion that somatic mutagenesis (micronuclear formation) after exposure to free radicals can be an adaptive response to this stress factor.Conclusion. Analysis leadsus to the conclusion that somatic mutagenesis (formation of micro‐nuclei) after exposure to free radicals can be an adaptive response to stress factor in habitat conditions in areas of liquidated prospecting wells of LUKOIL‐Nizhnevolzhskneft in the Northern Caspian Sea.