bluuebunny/crossref_metadata_2025_split huggingface dataset:
LINC01116 promotes proliferation and migration of endometrial stromal cells by targeting FOXP1 via sponging miR‐9‐5p in endometriosis
Authors: Cui Liangyi , Chen Silei , Wang Dandan , Yang Qing 
Published: December 2020

AbstractEndometriosis is a common multi‐factorial gynaecological disease. Recent studies have revealed that long non‐coding RNAs (lncRNAs) are involved in the pathogenesis of endometriosis. In the present study, the expression profiles of lncRNAs in 6 pairs of endometriosis ectopic endometrium (ecEM) and eutopic endometrium (euEM) tissues were analysed by RNA sequencing. From the profiles, LINC01116 was found to be up‐regulated in ecEM tissues compared to euEM tissues and was verified by quantitative real‐time PCR (qRT‐PCR). Then, functional experiments demonstrated that LINC01116 promoted the proliferation and migration of ectopic primary endometrial stromal cells (ESCs), while miR‐9‐5p exerted the opposite effects. Dual‐luciferase reporter assays verified that LINC01116 directly sponged miR‐9‐5p and relieved the suppression of its target, Forkhead box protein P1 (FOXP1). Rescue experiments further demonstrated that LINC01116 could promote proliferation and migration of ESCs by targeting FOXP1 via sponging miR‐9‐5p. Overall, our study illuminates that LINC01116 promotes the progression of endometriosis through the miR‐9‐5p/FOXP1 axis. This finding provides a novel therapeutic target for patients with endometriosis.
An integrated pan‐cancer analysis of TFAP4 aberrations and the potential clinical implications for cancer immunity
Authors: Liu Jian‐Nan , Kong Xiang‐Shuo , Sun Ping , Wang Rui , Li Wang , Chen Qi‐Feng 
Published: December 2020

AbstractStudies have shown that transcription factor activating enhancer binding protein 4 (TFAP4) plays a vital role in multiple types of cancer; however, the TFAP4 expression profile is still unknown, as is its value within the human pan‐cancer analysis. The present study comprehensively analysed TFAP4 expression patterns from 33 types of malignancies, along with the significance of TFAP4 for prognosis prediction and cancer immunity. TFAP4 displayed inconsistent levels of gene expression across the diverse cancer cell lines, and displayed abnormal expression within most malignant tumours, which closely corresponded to overall survival. More importantly, the TFAP4 level was also significantly related to the degree of tumour infiltration. TFAP4 was correlated using gene markers in tumour‐infiltrating immune cells and immune scores. TFAP4 expression was correlated with tumour mutation burden and microsatellite instability in different cancer types, and enrichment analyses identified TFAP4‐associated terms and pathways. The present study comprehensively analysed the expression of TFAP4 across 33 distinct types of cancers, which revealed that TFAP4 may possibly play a vital role during cancer formation and development. TFAP4 is related to differing degrees of immune infiltration within cancers, which suggests the potential of TFAP4 as an immunotherapy target in cancers. Our study demonstrated that TFAP4 plays an important role in tumorigenesis as a prognostic biomarker, which highlights the possibility of developing new targeted treatments.
PLCγ1 inhibition‐driven autophagy of IL‐1β‐treated chondrocyte confers cartilage protection against osteoarthritis, involving AMPK, Erk and Akt
Authors: Chen Xiaolei , Wang Yue , Qu Ning , Zhang Bing , Xia Chun 
Published: December 2020

AbstractPrevious studies identified the involvement of phosphoinositide‐specific phospholipase C (PLC) γ1 in some events of chondrocytes. This study aims to investigate whether and how PLCγ1 modulates autophagy to execute its role in osteoarthritis (OA) progression. Rat normal or human OA chondrocytes were pretreated with IL‐1β for mimicking or sustaining OA pathological condition. Using Western blotting, immunoprecipitation, qPCR, immunofluorescence and Dimethylmethylene blue assays, and ELISA and transmission electron microscope techniques, we found that PLCγ1 inhibitor U73122 enhanced Collagen II, Aggrecan and GAG levels, accompanied with increased LC3B‐II/I ratio and decreased P62 expression level, whereas autophagy inhibitor Chloroquine partially diminished its effect. Meanwhile, U73122 dissociated Beclin1 from Beclin1‐IP3R‐Bcl‐2 complex and blocked mTOR/ULK1 axis, in which the crosstalk between PLCγ1, AMPK, Erk and Akt were involved. Additionally, by haematoxylin and eosin, Safranin O/Fast green, and immunohistochemistry staining, we observed that intra‐articular injection of Ad‐shPLCγ1‐1/2 significantly enhanced Collagen and Aggrecan levels, accompanied with increased LC3B and decreased P62 levels in a rat OA model induced by anterior cruciate ligament transection and medial meniscus resection. Consequently, PLCγ1 inhibition‐driven autophagy conferred cartilage protection against OA through promoting ECM synthesis in OA chondrocytes in vivo and in vitro, involving the crosstalk between PLCγ1, AMPK, Erk and Akt.
Multigranularity cascade forest algorithm based on hyperspectral imaging to detect moisture content in Daqu
Authors: Huang Haoping , Hu Xinjun , Tian Jianping , Chen Ping , Huang Dan 
Published: December 2020

AbstractTo evaluate the quality of Daqu, the change of moisture content during the fermentation process is an important indicator. To improve the accuracy of measuring the moisture content, we studied a measuring method that used a near‐infrared hyperspectral imaging technique. In the process of using multigranularity cascade forest algorithm to do deep learning on the detection task, two improvements were made to improve detection accuracy. One was to establish a multigranularity cascade forest model with various scanning window combinations to solve the problem of distribution offset in the prediction results. The other was to do continuum removal processing to obtain enhanced spectral data from the sample spectral data. The results showed that the multigranularity cascade forest was markedly better than a support vector machine and a backpropagation neural network. The coefficient of determination of the prediction set (R2) and root mean square error of the Prediction set reached 0.9977 and 0.0021, respectively. The study results indicated that the hyperspectral imaging technique could achieve highly accurate detection and distribution visualization of the moisture content of Daqu during the fermentation process.Practical applicationsDaqu, a flavoring agent and fermentation starter, plays a vital role in the solid‐state liquor brewing process. During the fermentation process, moisture content is an important indicator for quality evaluation of Daqu. Therefore, it is highly important to detect the moisture content in Daqu quickly and accurately. The hyperspectral imaging technique, as an emerging detection technology, has advantages that traditional detection methods do not have, so that the disadvantages of traditional detection can be overcome. In our study, the hyperspectral imaging technique could achieve high‐precision detection of the moisture content of Daqu during the fermentation process, indicating that hyperspectral technology could be used to detect Daqu moisture content. Moreover, this method has great potential for real‐time indicators detection in winery for future work.
Exosomes in Parkinson disease
Authors: Pinnell Jennifer R. , Cui Mei , Tieu Kim 
Published: December 2020

AbstractParkinson disease (PD) is a prevalent neurodegenerative disease, in which the formation of misfolded and aggregated α‐synuclein is a key neuropathological hallmark. Recent studies reveal that extracellular vesicles such as exosomes present a potential mechanism for propagation of pathological α‐synuclein throughout the brain. The ability of exosomes to transport proteins and genetic material between cells, including mRNA and microRNAs which have been implicated in PD pathology, provides critical insights as to how exosomes may contribute to pathological progression in PD. Advances have also been made in the investigation of exosomes as potential tools for the modulation of Parkinson's pathology; their detection extracellularly may facilitate their use as biomarkers, while their small size could be utilised as vectors for the delivery of therapeutics. The aim of this review was to highlight our current knowledge of the role of exosomes in PD and potential clinical application.
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Enabling patient safety awareness using the Green Cross method: A qualitative description of users’ experience
Authors: Schwarz Anneli , Isaksson Stina , Källman Ulrika , Rusner Marie 
Published: December 2020

AbstractAimThe Green Cross method was developed to support healthcare staff in daily patient safety work. The aim of this study was to describe users’ experiences of the method when working with patient safety and their views on the core elements.BackgroundPatient safety systems need to be user‐friendly to facilitate learning from adverse events. The Green Cross method is described as a simple visual method to recognise risks and preventable adverse events (PAEs) in real time. There are no previous studies describing users’ experiences of the Green Cross method.DesignA qualitative descriptive design.Methods32 healthcare workers and managers from different specialties in a Swedish hospital were interviewed, from May–September 2018 about their experiences of the Green Cross method; either individually or as part of a group. The interviews were analysed using thematic analysis. The study follows the COREQ guidelines for qualitative data.ResultsParticipants associated the Green Cross method with patient safety, but no core elements of the method were identified. Instead, the opportunity to be engaged in patient safety work in a systematic way was underlined by all study participants. Highlighted key areas were the simplicity and the systematic framework of the method along with a need of distinct leadership. The daily meetings promoted trust and dialogue and developed the patient safety mindset. Daily meetings, together with the visualisation of the cross, were emphasised as important by users who otherwise had limited knowledge of the entire method.ConclusionThis study offers valuable information that can help deepen the understanding of how the method specifically supports patient safety work.Relevance to clinical practiceHealthcare workers are expected to report patient safety issues. This study presents user‐friendly aspects of the method as well as limitations, relevant for present and future users.
Cohabitation and Marital Dissolution: A Comment on Rosenfeld and Roesler (2019)
Authors: Manning Wendy D. , Smock Pamela J. , Kuperberg Arielle 
Published: December 2020

AbstractObjectiveOur goal is to comment on a recently published paper (Rosenfeld & Roesler, 2019. Journal of Marriage and Family, 81, 42‐58) and provide an alternative analysis of the association between premarital cohabitation and marital instability.BackgroundTheir findings run counter to recently published papers on this topic. Although their article offers a potential explanation for this finding, the models include multiple and potentially confounding measures of time creating questions about their conclusions.MethodOur comment is based on approaches used in prior studies of cohabitation and marital instability using the same data source. Reviews of measures of time and data limitations are included along with new event history analyses focusing on marriage cohorts.ResultsTheir models arguably include too many indicators of time. Unlike the study by Rosenfeld and Roesler (2019) and consistent with other recent studies, we find that cohabitation has a weaker association with dissolution among recently married couples and cohabitation with a spouse prior to marriage is not associated with marital instability for recent marriage cohorts. This finding holds even when accounting for variation by marital duration.ConclusionThis comment provides insights into the use of the National Survey of Family Growth data (
https://www.cdc.gov/nchs/nsfg/index.htm data) and measurement of time. Our results provide evidence that counters the conclusion by Rosenfeld and Roesler (2019) that scholars have been “misled” about the role of cohabitation and marital dissolution.
The ambiguities of self‐determination: IGAD and the secession of South Sudan
Authors: de Waal Alex 
Published: December 2020

AbstractThis paper examines the 1994 Declaration of Principles (DoP) for the resolution of the Sudanese civil war, adopted by the Inter‐Governmental Authority on Development (IGAD). This was the only occasion on which an African inter‐state organization included separation as an option for resolving a civil war. It was the basis for South Sudan's independence in 2011. The DoP was drafted by the Ethiopian government, and imposed on belligerent parties, both of which were, at the time, unionist. The paper identifies two concepts of self‐determination within the DoP— independence for colonial territories and the Marxist‐Leninist idea of self‐determination for national groups. The rationale for including both arose from Ethiopian leadership within IGAD. The paper also examines the diverse Sudanese debates on self‐determination, including several strands of nationalism, Islamism, and the ‘New Sudan’ of the Sudan People's Liberation Movement (SPLM). There was radical disagreement among Sudanese on national identity and self‐determination, creating ambiguities that ironically facilitated the exercise in southern self‐determination in 2011. Drawing on documentation of Sudanese negotiations, the paper examines how the DoP unlocked the Sudanese debate on the issue, and how the different concepts fared up to the time of the independence of South Sudan.
Lactobacilli postbiotics reduce biofilm formation and alter transcription of virulence genes of Aggregatibacter actinomycetemcomitans
Authors: Ishikawa Karin H. , Bueno Manuela R. , Kawamoto Dione , Simionato Maria R. L. , Mayer Marcia P. A. 
Published: December 2020

AbstractPeriodontitis is characterized by a dysbiotic microbial community and treatment strategies include the reestablishment of symbiosis by reducing pathogens abundance. Aggregatibacter actinomycetemcomitans (Aa) is frequently associated with rapidly progressing periodontitis. Since the oral ecosystem may be affected by metabolic end‐products of bacteria, we evaluated the effect of soluble compounds released by probiotic lactobacilli, known as postbiotics, on Aa biofilm and expression of virulence‐associated genes. Cell‐free pH‐neutralized supernatants (CFS) of Lactobacillus rhamnosus Lr32, L. rhamnosus HN001, Lactobacillus acidophilus LA5, and L. acidophilus NCFM were tested against a fimbriated clinical isolate of Aa JP2 genotype (1 × 107 CFU/well) on biofilm formation for 24 hr, and early and mature preformed biofilms (2 and 24 hr). Lactobacilli CFS partially reduced Aa viable counts and biofilms biomass, but did not affect the number of viable non‐adherent bacteria, except for LA5 CFS. Furthermore, LA5 CFS and, in a lesser extent HN001 CFS, influenced Aa preformed biofilms. Lactobacilli postbiotics altered expression profile of Aa in a strain‐specific fashion. Transcription of cytolethal distending toxin (cdtB) and leukotoxin (ltxA) was downregulated by CFS of LA5 and LR32 CFS. Although all probiotics produced detectable peroxide, transcription of katA was downregulated by lactobacilli CFS. Transcription of dspB was abrogated by LR32 and NCFM CFS, but increased by HN001, whereas expression of pgA was not affected by any postbiotic. Our data indicated the potential of postbiotics from lactobacilli, especially LA5, to reduce colonization levels of Aa and to modulate the expression of virulence factors implicated in evasion of host defenses.
Dealing with vulnerability to carbon emission from gas flaring: the roles of transparency and utilisation policies in Nigeria
Authors: Hassan Aminu 
Published: December 2020

AbstractGas flaring, a practice described as environmentally damaging and financially wasteful, still persists on a large scale in the Nigerian oil and gas industry. Firstly, this paper investigates the vulnerability of the Nigerian economic performance to environmental pollution by estimating and analysing the relationship between carbon emission from gas flaring and Nigeria’s GDP. Secondly, the study examines how transparency and gas utilisation policies, deployed by the country, are performing in dealing with vulnerability to carbon emission from gas flaring. The paper uses an autoregressive distributed lag bounds testing approach to cointegration and error‐correction‐based Granger causality. The paper establishes that carbon emission from gas flaring affects Nigeria’s GDP negatively in both the short and long run. However, the results show that GDP has started exhibiting resilience to carbon emission from gas flaring in the long run. Findings imply that even though transparency and gas utilisation policies are doing well in increasing GDP’s long‐run resilience, persistent carbon emission from gas flaring continues to cause short‐run and long‐run economic vulnerability. The study discusses policy implications meant to be useful to the Nigerian government and the relevant regulatory authorities in the country's oil and gas industry.
Cryoablation for pulmonary veins isolation in obese patients with atrial fibrillation compared to nonobese patients
Authors: Malaspina Daniele , Brasca Francesco , Iacopino Saverio , Arena Giuseppe , Molon Giulio , Pieragnoli Paolo , Tondo Claudio , Manfrin Massimiliano , Rovaris Giovanni , Verlato Roberto , Dello Russo Antonio , Startari Umberto , Sgarito Giuseppe , Perego Giovanni Battista 
Published: December 2020

AbstractBackgroundPulmonary veins isolation (PVI) by cryoballoon (CB) ablation is marginally represented in clinical studies in obese patients. The aim of this analysis was to evaluate the safety and efficacy of CB‐PVI in a large cohort of overweight and obese patients from the 1STOP project.MethodsFrom 2012 to 2018, 2048 patients with atrial fibrillation (AF) (70% male, 59 ± 11 years; 75% paroxysmal AF) underwent index CB‐PVI. The patient data were separated into three cohorts for statistical evaluation, including: normal weight (body mass index [BMI] < 25 kg/m2), overweight (BMI = 25‐30 kg/m2), and obese patients (BMI > 30 kg/m2).ResultsOut of 2048 patients, 693 (34%) patients had a BMI < 25 and were deemed as normal. There were 944 (46%) patients categorized as overweight (BMI = 25‐30) and 411 (20%) as obese (BMI > 30). Overweight or obese patients were more often in persistent AF, had more frequently hypertension and diabetes, had higher CHA2DS2‐VASc score, and had a number of failed antiarrhythmic drug (AAD). Periprocedural complication rates were similar among the three cohorts. The 12‐month freedom from AF recurrence was 76.4% in the normal BMI group as compared to 79.2% in the overweight and 73.5% in the obese group (p = .35). However, 48% of overweight patients were on AAD treatment during the follow‐up. By multivariate analysis, BMI was not a predictor for AF recurrence following the index CB‐PVI.ConclusionCB‐PVI in obese patients is a safe procedure. Increased BMI (either moderate or severe) does not seem to be associated with a worse outcome or to a different rate of AAD discontinuation at 12 months.
Risk‐adapted therapy for the management of cytokine release syndrome in children undergoing unmanipulated haploidentical stem cell transplantation
Authors: Jayakumar Indira , Uppuluri Ramya , Lakshmanan Chidhambharam , Kumar Gowdhaman Anand , Vellaichamy Swaminathan Venkateswaran , Raj Revathi 
Published: December 2020

AbstractBackgroundWe aimed to describe an algorithm for the management of cytokine release syndrome (CRS) associated with haploidentical hematopoietic stem cell transplantation (haploSCT).Patients and methodsWe performed a prospective study where children up to 18 years of age undergoing haploSCT with post‐transplant cyclophosphamide from September 2014 to March 2020 were included. Supportive care included low‐dose adrenaline, high‐flow nasal cannula, and N‐acetylcysteine (NAC). Methylprednisolone and tocilizumab were administered in the peri‐engraftment phase for grade 2 CRS or one‐log increase and grade 3 CRS or a two‐log increase in ferritin, respectively.ResultsData were analyzed in 135/148 children as 13 children died before engraftment due to sepsis. CRS was noted in 97% transplants (grade 1—74.1%, grade 2—15.6%, grade 3—6.7%, grade 4—1.4%). Grade 2 and above CRS was higher in non‐malignant conditions (33% vs 13%, P‐value .009). The percentage median rise in ferritin was 129%—grade 1, 171%—grade 2, and 344%—grade 3. Seven children received tocilizumab, and two of whom had ferritin values greater than 100 000 ng/mL with no mortality in this group. Low‐dose adrenaline, high‐flow nasal cannula, and ventilator support were needed in 13%, 10%, and 4%, respectively. Mortality in our cohort was 3/135 (2.2%), with two deaths due to sepsis and one due to grade 4 CRS.ConclusionsA risk‐stratified approach using steroids in grade 2 and tocilizumab in grade 3/4 in the setting of haploSCT with NAC infusion and early use of low‐dose adrenaline and HFNC can help provide adequate control of CRS, thereby ensuring optimal outcomes and survival.
Plasma Treatment as a Promising Environmentally Benign Approach for Synthesis of Valuable Multi‐gas Doped Nano‐TiO2‐P25: An Efficient Way to Boost the Photocatalytic Performance under Visible Light Illumination
Authors: Hosseini Saber , Amoozadeh Ali 
Published: December 2020

AbstractAn ingenious prospect has been established to synthesize a wide range of non‐metal‐doped TiO2‐P25 by plasma technique. Different atmospheres (Air, O2, N2, Ar and CO2) have been embedded on the surface of TiO2‐P25 by plasma treating as an effective alternative to wet chemical pretreatment processes. This approach is clean beyond recognition by employing pure gases as well as no need to poison precursors or organic solvents without producing waste stream, which surprisingly can meet green chemistry purposes. More specifically, plasma has been a contributing factor in the narrowing band gap energies of doped photocatalysts in comparison with pure TiO2‐P25. Synthesized photocatalysts gained enormous benefit from the plasma treatment in the selective oxidation of benzyl alcohols to associating aldehydes under blue LED illumination with excellent yields, which dramatically decreased the time reaction to many folds. Additionally, benzaldehyde formation under influence of various wavelengths of visible light, including blue photons (λmax = 460 nm), green photons (λmax = 510 nm) and red photons (λmax = 630 nm) was compared to assess the effect of plasma treating on photoactivity of nano‐TiO2‐P25. Furthermore, as‐prepared photocatalysts were investigated by diverse characterization techniques.