bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of a prospective payment method for health facilities on direct medical expenditures in a low-resource setting: a paired pre-post study
Authors: Meda Ivlabèhiré Bertrand , Kouanda Seni , Dumont Alexandre , Ridde Valéry 
Published: April 2020

Abstract
Almost all sub-Saharan countries have adopted cost-reduction policies to facilitate access to health care. However, several studies underline the reimbursement delays experienced by health facilities, which lead to deficient implementation of these policies. In April 2016, for its free care policy, Burkina Faso shifted from fee-for-service (FFS) paid retrospectively to FFS paid prospectively. This study tested the hypothesis that this new method of payment would be associated with an increase in direct medical expenditures (expenses covered by the policies) associated with deliveries. This paired pre-post study used data from two cross-sectional national surveys. Observations were paired according to the health facility and the type of delivery. We used a combined approach (state and household perspectives) to capture all direct medical expenses (delivery fees, drugs and supplies costs, paraclinical exam costs and hospitalization fees). A Wilcoxon signed-rank test was used to test the hypothesis that the 2016 distribution of direct medical expenditures was greater than that for 2014. A total of 279 pairs of normal deliveries, 66 dystocia deliveries and 48 caesareans were analysed. The direct medical expenditure medians were USD 4.97 [interquartile range (IQR): 4.30–6.02], 22.10 [IQR: 15.59–29.32] and 103.58 [IQR: 85.13–113.88] in 2014 vs USD 5.55 [IQR: 4.55–6.88], 23.90 [IQR: 17.55–48.81] and 141.54 [IQR: 104.10–172.02] in 2016 for normal, dystocia and caesarean deliveries, respectively. Except for dystocia (P = 0.128) and medical centres (P = 0.240), the 2016 direct medical expenditures were higher than the 2014 expenses, regardless of the type of delivery and level of care. The 2016 expenditures were higher than the 2014 expenditures, regardless of the components considered. In the context of cost-reduction policies in sub-Saharan countries, greater attention must be paid to the provider payment method and cost-control measures because these elements may generate an increase in medical expenditures, which threatens the sustainability of these policies.
Rapid development of telehealth capabilities within pediatric patient portal infrastructure for COVID-19 care: barriers, solutions, results
Authors: Patel Pious D , Cobb Jared , Wright Deidre , Turer Robert W , Jordan Tiffany , Humphrey Amber , Kepner Adrienne L , Smith Gaye , Rosenbloom S Trent 
Published: April 2020

Abstract
The COVID-19 national emergency has led to surging care demand and the need for unprecedented telehealth expansion. Rapid telehealth expansion can be especially complex for pediatric patients. From the experience of a large academic medical center, this report describes a pathway for efficiently increasing capacity of remote pediatric enrollment for telehealth while fulfilling privacy, security, and convenience concerns.
The design and implementation of the process took 2 days. Five process requirements were identified: efficient enrollment, remote ability to establish parentage, minimal additional work for application processing, compliance with guidelines for adolescent autonomy, and compliance with institutional privacy and security policies.
Weekly enrollment subsequently increased 10-fold for children (age 0–12 years) and 1.2-fold for adolescents (age 13–17 years). Weekly telehealth visits increased 200-fold for children and 90-fold for adolescents.
The obstacles and solutions presented in this report can provide guidance to health systems for similar challenges during the COVID-19 response and future disasters.
Bilastine: Quantitative Determination by LC with Fluorescence Detection and Structural Elucidation of the Degradation Products Using HRMS
Authors: Motta Paola Ribeiro , dos Santos Porto Douglas , Martini Paulo Roberto Rodrigues , Bajerski Lisiane , Azeredo Juliano Braun , Paula Fávero Reisdorfer , Paim Clésio Soldateli 
Published: April 2020

Abstract

Background
A liquid chromatography (LC) stability-indicating method was developed and validated for the quantitative determination of bilastine in coated tablets.

Objective
The procedure was validated for specificity, linearity, robustness, precision, and accuracy. Plackett-Burmann experimental design was used to determine the robustness of the method.

Method
Chromatographic separation was performed on a Shim-pack® RP-18 column with fluorescence detection. The degradation products formed under oxidative conditions were isolated and identified using high-resolution mass spectrometry (HRMS). In silico prediction of degradation products and in silico toxicity studies were also performed.

Results
The LC method presented good recovery and precision (intraday and interday), the response was linear in a range of 0.20 to 0.70 μg mL−1, and the results demonstrated the robustness of the analytical method under the evaluated conditions.

Conclusions
The degradation products were identified as benzimidazole (DP1) and amine N-oxide of bilastine (DP2). The results for the toxicity studies demonstrated the high mutagenic potential of DP1 and hepatotoxicity and hERG I inhibitor effects of DP2.

Highlights
Bilastine degradation products were identified as benzimidazole and amine N-oxide using HRMS.
Breast Imaging and Image-guided Intervention in Tanzania: Initial Experience
Authors: Okello Roselyne , Rooney Timothy B , Jumbe Mwajuma , Sakafu Lulu , Koch Troy , Laage-Gaupp Fabian , Minja Frank , Chamberlin Mary 
Published: April 2020

Abstract
Breast imaging capacity in Tanzania is currently very limited. In a country of almost 60 million people, mammographic units are exceedingly rare. The few existing units are compromised by lack of maintenance and quality control and extremely limited technologist training. Breast cancer incidence continues to increase in East Africa, while the ability to accurately diagnose and differentiate benign and malignant breast disease remains a challenge. However, as ultrasound technology improves and becomes more affordable, there is increased access to devices including hand-held point of care ultrasound. Additionally, there has been a recent expansion in the training of academic radiologists in Tanzania, creating an opportunity for possible sustainable improvement of breast imaging and intervention. Our initial efforts at the Muhimbili Academic Medical Center (MAMC) included social media advertising for symptomatic and asymptomatic women, followed by screening (clinical breast exam) and subsequent ultrasound imaging of symptomatic women. We then initiated a workflow for ultrasound-guided breast intervention, performed biopsies, reviewed results by local pathologists, and brought the postbiopsy patients back for radiologic–pathologic correlation, wound assessment, and coordination of follow-up and treatment.
Using IPM-CPR as a Management Program for Apple Orchards
Authors: Akotsen-Mensah C , Blaauw B , Short B , Leskey T C , Bergh J C , Polk D , Nielsen A L 
Published: April 2020

Abstract
We have demonstrated how management of key orchard pests including the insect invasive species Halyomorpha halys (Stål) (Hemiptera: Pentatomidae) can be accomplished using a systems-level approach termed IPM-CPR (Integrated Pest Management-Crop Perimeter Restructuring) in apple. We conducted on-farm comparisons of IPM-CPR to standard management program for managing H. halys, Cydia pomonella (L.) (Lepidoptera: Tortricidae), Grapholita molesta (Busck) (Lepidoptera: Tortricidae), and Lygus lineolaris Palisot de Beauvois (Hemiptera: Miridae) in commercial apple orchards in 2014, 2016, and 2017 in New Jersey, Maryland, and Virginia. The presence and abundance of key pests and fruit injury at harvest were used as a measure of success of the program. We compared the amount of insecticide applied for each management program. In majority of instances, there were no differences in the IPM-CPR and the standard management program in terms of H. halys numbers in baited pyramid traps and stink bug injury at harvest. Damage from C. pomonella and G. molesta in the IPM-CPR treatment was significantly lower than the standard management program in 2014 and 2017. Amount of active ingredient used was on average 62.1% lower in the IPM-CPR treatment compared with standard management program. Despite a reduction in insecticide use, there were minimal impacts on beneficial insects. Overall, IPM-CPR in apples successfully managed key orchard pests, including H. halys, and used significantly less insecticide than a standard insecticide-based management program and could be adopted as a systems-level approach for pest population reduction.
Outcomes after thoracoscopic surgery in octogenarian patients with clinical N0 non-small-cell lung cancer
Authors: Nakao Masayuki , Ichinose Junji , Matsuura Yosuke , Okumura Sakae , Mun Mingyon 
Published: April 2020

Abstract

Objective
The number of surgeries for elderly patients with lung cancer is increasing. In our institute, thoracoscopic lobectomy and hilar lymph node dissection are the standard procedure for octogenarian patients with clinical N0 non-small-cell lung cancer. The aim of this study was to determine the outcome of our strategy for octogenarian patients.

Methods
Seventy octogenarian patients with clinical N0 non-small-cell lung cancer who underwent surgery were enrolled (O group). As a control group, 205 septuagenarian patients were also enrolled (S group). We compared several clinicopathological factors and outcomes.

Results
The median age of the O group was 82. There was no significant difference in the comorbidity ratio between the two groups. The 5-year overall survival ratio for the O group (72.8%) was significantly worse than that for the S group (88.3%). However, multivariate analysis proved age was not an independent predictor of outcome. The rates of recurrences involving ipsilateral mediastinal lymph nodes were equal in the two groups. After propensity score matching, clinical T1 patients were dominant (85%) in two matched group and no statistically significant differences were observed in the 5-year overall survival between the two groups.

Conclusions
Our strategy for octogenarian patients with non-small-cell lung cancer, including omission of mediastinal lymph node dissection, was determined to be feasible, in particularly with cT1N0 disease.
Hippocampal Lipocalin 2 Is Associated With Neuroinflammation and Iron-Related Oxidative Stress in ob/ob Mice
Authors: Jin Zhen , Kim Kyung Eun , Shin Hyun Joo , Jeong Eun Ae , Park Kyung-Ah , Lee Jong Youl , An Hyeong Seok , Choi Eun Bee , Jeong Jae Hun , Kwak Woori , Roh Gu Seob 
Published: April 2020

Abstract
Obesity causes brain injuries with inflammatory and structural changes, leading to neurodegeneration. Although increased circulating lipocalin 2 (LCN2) level has been implicated in neurodegenerative diseases, the precise mechanism of neurodegeneration in obesity is not clear. Here, we investigated whether LCN2-mediated signaling promotes neurodegeneration in the hippocampus of leptin-deficient ob/ob mice, which are characterized by obesity, insulin resistance, systemic inflammation, and neuroinflammation. In particular, there was significant upregulation of both LCN2 and matrix metalloproteinase 9 levels from serum and hippocampus in ob/ob mice. Using RNA-seq analysis, we found that neurodegeneration- sortilin-related receptor 1 (Sorl1) and brain-derived neurotrophic factor (Bdnf) genes were significantly reduced in the hippocampus of ob/ob mice. We additionally found that the endosome-related WD repeat and FYVE-domain-containing 1 (Wdfy1) gene were upregulated in ob/ob mice. In particular, iron overload-related mitochondrial ferritin and nuclear factor erythroid 2-related factor 2 (Nrf2)/heme oxygenase-1 (HO-1) proteins were increased in the hippocampus of ob/ob. Thus, these findings indicate that iron-binding protein LCN2-mediated oxidative stress promotes neurodegeneration in ob/ob mice.
Caffeine Use and Associations With Sleep in Adolescents With and Without ADHD
Authors: Cusick Caroline N , Langberg Joshua M , Breaux Rosanna , Green Cathrin D , Becker Stephen P 
Published: April 2020

Abstract

Objective
The objective of this study was to compare caffeine consumption in the morning, afternoon, and evening in adolescents with and without attention-deficit/hyperactivity disorder (ADHD) and examine associations with sleep functioning.

Methods
Participants were 302 adolescents (ages 12–14) with (N = 140) and without (N = 162) ADHD. Adolescents wore actigraph watches to assess total sleep time and wake after sleep onset and reported on sleep–wake problems and the number of caffeinated beverages consumed per day in the morning, afternoon, and evening. Parents reported on adolescents’ difficulties initiating and maintaining sleep. Chi-square tests, odds ratios, and path analyses were conducted.

Results
Analyses controlled for sex, medication status, and pubertal development. Adolescents with ADHD were 2.47 times more likely to consume caffeine in the afternoon and evening than adolescents without ADHD. Path analyses indicated significant associations between afternoon caffeine use and more self-reported sleep problems for adolescents with and without ADHD, and an association between evening caffeine use and self-reported sleep problems only in adolescents with ADHD. Afternoon caffeine use was associated with parent-reported sleep problems in adolescents with ADHD only. Caffeine use was not associated with actigraphy-assessed sleep.

Conclusion
This is the first study to show that adolescents with ADHD consume more caffeine than peers during later times of the day. Additionally, caffeine use is more consistently associated with poorer subjective sleep functioning in adolescents with ADHD. Pediatricians and mental health professionals should assess for caffeine use in adolescents with ADHD and co-occurring sleep problems.
Kilonova rates from spherical and axisymmetrical models
Authors: Kóbori József , Bagoly Zsolt , Balázs Lajos G 
Published: April 2020

ABSTRACT
Detecting the thermal emission from double neutron star merger events is a challenging task because of the quick fading of the observed flux. In order to create an efficient observing strategy for their observing method, it is crucial to know their intrinsic rate. Unfortunately, the numerous models existing today predict this rate on a very wide range. Hence, our goal in this paper is to investigate the effect of different levels of approximations on the relative rate predictions. Also, we study the effect of distinct ejecta mass layouts on the light curve. We find that the ratio of the expected kilonova detections of the spherical to axisymmetrical models is 6:1 (or 2:1, depending on the input parameter set applied in our work). Nevertheless, the light-curve shape is only slightly affected by the various ejecta alignments. This means that different ejecta layouts can produce light curves with similar shapes making it a challenging task to infer the structure of the matter outflow. Thus, we conclude that the uncertainty in the rate predictions arising from the various ejecta mass distribution models is negligible compared to the errors present in other input parameters (e.g. binary neutron star merger rate). In addition, we show that up to moderate redshifts (z ≲ 0.2) the redshift distribution type (observed or uniform in volume) does not affect the expected relative rate estimations.
Role of ambient wavelength, shell size and pigmentation intensity in the heating of Mediterranean Theba pisana (Eupulmonata: Helicidae)
Authors: Tull Tatjana , Schmidt Danina , Köhler Heinz-R 
Published: April 2020

ABSTRACT
Theba pisana is a polymorphic land snail widely used in ecophysiological research on the biological significance of solar irradiation. We investigated whether, taking size into account, differently pigmented morphs of a Mediterranean population of T. pisana influence the snails’ heating under different wavelengths. We used a laboratory set-up comprising light-emitting diodes emitting visible light of defined wavelengths and quantified shell heating using high-resolution thermography. Shell pigmentation was quantified densitometrically. There were significant effects of both shell size and light wavelength, such that small snails heated more than large ones over 15 min, and blue light (470 nm) raised temperature more than green light (525 nm). Pigmentation alone did not show significance, but a trend towards higher temperature with increasing pigmentation was evident. Despite the observed significances and trends, we could only explain at most 4.67% of variation in shell heating by multiple regression modelling. However, the light intensity used in this experiment was low and the pigmentation intensity of the shells varied, at most, by a factor of 2. This suggests the actual temperature of a snail depends not only on the tested variables, but also on the multifactorial action of a number of unknown, perhaps intrinsic physiological parameters.
Phase-plate cryo-EM structure of the Widom 601 CENP-A nucleosome core particle reveals differential flexibility of the DNA ends
Authors: Boopathi Ramachandran , Danev Radostin , Khoshouei Maryam , Kale Seyit , Nahata Sunil , Ramos Lorrie , Angelov Dimitar , Dimitrov Stefan , Hamiche Ali , Petosa Carlo , Bednar Jan 
Published: April 2020

AbstractThe histone H3 variant CENP-A marks centromeres epigenetically and is essential for mitotic fidelity. Previous crystallographic studies of the CENP-A nucleosome core particle (NCP) reconstituted with a human α-satellite DNA derivative revealed both DNA ends to be highly flexible, a feature important for CENP-A mitotic functions. However, recent cryo-EM studies of CENP-A NCP complexes comprising primarily Widom 601 DNA reported well-ordered DNA ends. Here, we report the cryo-EM structure of the CENP-A 601 NCP determined by Volta phase-plate imaging. The data reveal that one (‘left’) 601 DNA end is well ordered whereas the other (‘right’) end is flexible and partly detached from the histone core, suggesting sequence-dependent dynamics of the DNA termini. Indeed, a molecular dynamics simulation of the CENP-A 601 NCP confirmed the distinct dynamics of the two DNA extremities. Reprocessing the image data using two-fold symmetry yielded a cryo-EM map in which both DNA ends appeared well ordered, indicating that such an artefact may inadvertently arise if NCP asymmetry is lost during image processing. These findings enhance our understanding of the dynamic features that discriminate CENP-A from H3 nucleosomes by revealing that DNA end flexibility can be fine-tuned in a sequence-dependent manner.
Generation and validation of structurally defined antibody–siRNA conjugates
Authors: Nanna Alex R , Kel’in Alexander V , Theile Christopher , Pierson Justin M , Voo Zhi Xiang , Garg Ashish , Nair Jayaprakash K , Maier Martin A , Fitzgerald Kevin , Rader Christoph 
Published: April 2020

Abstract
Gene silencing by RNA interference (RNAi) has emerged as a powerful treatment strategy across a potentially broad range of diseases. Tailoring siRNAs to silence genes vital for cancer cell growth and function could be an effective treatment, but there are several challenges which must be overcome to enable their use as a therapeutic modality, among which efficient and selective delivery to cancer cells remains paramount. Attempts to use antibodies for siRNA delivery have been reported but these strategies use either nonspecific conjugation resulting in mixtures, or site-specific methods that require multiple steps, introduction of mutations, or use of enzymes. Here, we report a method to generate antibody–siRNA (1:2) conjugates (ARCs) that are structurally defined and easy to assemble. This ARC platform is based on engineered dual variable domain (DVD) antibodies containing a natural uniquely reactive lysine residue for site-specific conjugation to β-lactam linker-functionalized siRNA. The conjugation is efficient, does not compromise the affinity of the parental antibody, and utilizes chemically stabilized siRNA. For proof-of-concept, we generated DVD-ARCs targeting various cell surface antigens on multiple myeloma cells for the selective delivery of siRNA targeting β-catenin (CTNNB1). A set of BCMA-targeting DVD-ARCs at concentrations as low as 10 nM revealed significant CTNNB1 mRNA and protein knockdown.
Quality of supervisor behaviour, workplace social capital and psychological well-being
Authors: Kizuki Masashi , Fujiwara Takeo 
Published: April 2020

Abstract

Background
Employees with a higher level of workplace social capital (i.e. relationships, trust and reciprocity at work) have a lower risk of mental health problems. Supervisor behaviour may be a predictor of workplace social capital.

Aims
To examine the associations between supervisor behaviour, workplace social capital and psychological well-being.

Methods
We conducted a secondary analysis of the sixth European Working Conditions Survey. The study sample included 28 900 employees in 35 European countries with an immediate line manager. Depression was assessed with the WHO-5 Well-Being Index. Supervisor behaviour quality and workplace social capital were each measured with a 6-item question. Association between quality of supervisor behaviour and workplace social capital was analysed using a hierarchical linear modelling. A mediation analysis was conducted by using hierarchical logistic models of depression with and without workplace social capital index.

Results
A higher supervisor behaviour quality index was associated with an increased workplace social capital index (β, 0.55; 95% confidence interval [CI], 0.51–0.59). A higher workplace social capital index was associated with lower odds of depression (odds ratio [OR], 0.89; 95% CI, 0.87–0.90). A higher supervisor behaviour quality index was associated with lower odds of depression (OR, 0.90; 95% CI, 0.89–0.92); 58% of the effect was mediated by workplace social capital.

Conclusions
Our findings support the hypothesis that better supervisor behaviour quality increases workplace social capital and contributes to psychological well-being of employees. This may be useful for planning organizational interventions to enhance mental health of workers.