bluuebunny/crossref_metadata_2025_split huggingface dataset:
First evidence of the mutations associated with pyrethroid resistance in head lice (Phthiraptera: Pediculidae) from Honduras
Authors: Larkin Kelsey , Rodriguez Carol A. , Jamani Shabana , Fronza Georgina , Roca-Acevedo Gonzalo , Sanchez Ana , Toloza Ariel C. 
Published: June 2020

Abstract
Background
The human head louse, Pediculus humanus capitis, is a cosmopolitan blood-sucking ectoparasite affecting mostly schoolchildren in both developed and developing countries. In Honduras, chemical pediculicides are the first line of treatment, with permethrin as their main active ingredient. Despite the extended use of these products, there is currently no research investigating insecticide resistance in Honduran head lice. In head lice, the most common mechanism is knockdown resistance (kdr), which is the result of two point mutations and the associated amino acid substitutions, T917I and L920F, within the voltage-sensitive sodium channel (VSSC).

Methods
Genomic DNA was extracted from 83 head lice collected in the localities of San Buenaventura and La Hicaca, Honduras. Polymerase chain reaction (PCR) was used to amplify a 332-bp fragment of the VSSC gene that contains a site affected by C/T mutation which results in a T917I amino acid substitution on each human head louse genomic DNA fragments.

Results
The C/T non-synonymous mutation which results in the T917I kdr amino acid substitution was detected in both head lice populations at frequencies ranging between 0.45–0.5. Globally, the frequency of this substitution was 0.47. Of these, 5 (6.1%) were homozygous susceptible and 78 (93.9%) were heterozygotes. The kdr-resistant homozygote (RR) was not detected in the studied populations. Thus, 93.9% of the head lice collected in Honduras harbored only one T917I allele. Exact test for the Hardy-Weinberg equilibrium for both localities showed that genotype frequencies differed significantly from expectation. In addition, San Buenaventura and La Hicaca populations had an inbreeding coefficient (Fis) < 0, suggesting an excess of heterozygotes.

Conclusions
To our knowledge, this is the first study showing the presence of the C/T mutation responsible of the T917I kdr allele associated with pyrethroid resistance in P. h. capitis from Honduras. The PCR-restriction fragment length polymorphism (RFLP) employed here has demonstrated to be a reliable, economic, and reproducible assay that can be used to accurately genotype individual head lice for the mutation encoding the resistance-conferring T917I amino acid substitution. This highlights the necessity of proactive resistance management programmes designed to detect pyrethroid mutations before they become established within populations of head lice.
Longitudinal immune cell monitoring identified CD14++ CD16+ intermediate monocyte as a marker of relapse in patients with ANCA-associated vasculitis
Authors: Matsumoto Kotaro , Suzuki Katsuya , Yoshimoto Keiko , Seki Noriyasu , Tsujimoto Hideto , Chiba Kenji , Takeuchi Tsutomu 
Published: June 2020

Abstract
Background
Anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV) is an autoimmune disease that affects small- to medium-sized blood vessels. Despite treatments having been improved, patients often experience disease relapses. It remains unclear how the immune cells involve in the development of vasculitis and how they fluctuate over the course of treatment. In this study, we aimed to identify the immune subsets and serum cytokines associated with disease relapse by comprehensive immuno-phenotyping in AAV patients.

Methods
We reviewed consecutive patients (n = 29) from Keio University Hospital who had been newly diagnosed with AAV from January 2015 to February 2019 and chronologically followed until 52 weeks. Numbers of circulating T cells, B cells, monocytes, and granulocytes were analyzed by flow cytometry (FACS). Serum levels of cytokines were measured by electrochemiluminescence enzyme immunoassay. Clinical information was obtained from patients’ records and association with time-course changes in immuno-phenotypes and serum levels of cytokines were assessed.

Results
Comprehensive immuno-phenotyping data from 161 samples from 29 AAV patients at diagnosis; at weeks 4, 12, 24, and 52 of treatment; and at time of major relapse were examined. FACS analysis from patients with relapse revealed that CD14++ CD16+ intermediate monocytes and plasma cells concomitantly changed associated with disease relapse, which were independent from treatment regimen, ANCA status, or disease phenotype. In particular, the number of CD14++ CD16+ intermediate monocytes at relapse was significantly higher than that in remission or in healthy controls. Serum cytokine measurement revealed that changes of monocyte-derived proinflammatory cytokines such as IL-1β, IL-6, IL-8, and TNF-α were associated with disease status.

Conclusions
Chronological changes in CD14++ CD16+ intermediate monocyte counts can be a marker of disease relapse in AAV patients.
MicroRNA-320d regulates tumor growth and invasion by promoting FoxM1 and predicts poor outcome in gastric cardiac adenocarcinoma
Authors: Chen Xiaojie , Gao Shegan , Zhao Zhiwei , Liang Gaofeng , Kong Jinyu , Feng Xiaoshan 
Published: June 2020

AbstractRecent evidences demonstrate that dysregulated expression of microRNA-320d (miR-320d) has been associated with several cancer development and progression. However the effect of miR-320d on gastric cardiac adenocarcinoma (GCA) and the association of miR-320d with its potential gene target FoxM1 remain unclear. Here, we evaluated expression profile of miR-320d and FoxM1 in 60 human GCA tissues and GCA cell lines (OE-19 and SK-GT2). Immunohistochemistry, qualitative PCR and western-blotting were performed in GCA tissues to detect the expression level of miR-320d and FoxM1. CCK-8, transwell, wound-healing assays, and in vivo experiments were conducted using GCA cells that treated with miR-320d mimics or inhibitors to evaluate the biological functions of miR-320d. Luciferase reporter assay was conducted to confirm possible binding sites of FoxM1 for miR-320d. Compared with paired non-cancerous tissues, it showed that miR-320d expression was significantly decreased in GCA specimens (P < 0.0001), while FoxM1 was significantly upregulated in GCA tissues (P < 0.0001). Modulating miR-320d function by transfection of miR-320 mimics or inhibitor led to inhibition or promotion of GCA cell proliferation and invasion, thus regulating tumor progression in GCA-tumor bearing mice. The mechanism analysis of miR-320d/FoxM1 showed that FoxM1 has two miR-320d binding sites in its 3′-untranslated region (3′-UTR), that contributes to regulation of the cell biological behaviors. Taken together, our data suggested that miR-320d acts as a tumor suppressor in GCA by directly targeting FoxM1 and thus potentially serves as a biomarker for anti-GCA therapy in GCA patients.
Visible light communication-based traffic control of autonomous vehicles at multi-lane roundabouts
Authors: Fakirah Maged , Leng Supeng , Chen Xiaosha , Zhou Jihua 
Published: June 2020

AbstractRoundabouts are a form of circular intersections adopted to secure road safety of the vehicles. However, collisions are not prevented entirely even when using roundabouts, since accidents may happen at specific intersection points leading to either traffic congestions or serious accidents. In this paper, a visible light communication (VLC)-based collision avoidance scheme is proposed to coordinate autonomous vehicles in traversing roundabouts, which has high effectiveness in vehicular environments. In particular, roadside units (RSUs) are deployed at the roundabout entrances to coordinate the vehicles in a vehicle-to-infrastructure (V2I) mode. By adopting the synchronization approach, vehicles can pass the roundabout simultaneously if their paths are concurrent with each other. Otherwise, vehicles are prioritized according to their arrival time and reasonable decelerations are applied to waive potential conflicts. Simulation results showed that our proposed approach satisfies the roundabout traffic demands in terms of concurrency, safety, and time utilization as vehicles are strongly recommended to decelerate in only 22% of the cases studied in different scenarios.
Quasilinear asymptotically periodic Schrödinger–Poisson system with subcritical growth
Authors: Zhang Jing , Guo Lifeng , Yang Miaomiao 
Published: June 2020

AbstractThe aim of this paper is establishing the existence of a nontrivial solution for the following quasilinear Schrödinger–Poisson system: $$ \left \{ \textstyle\begin{array}{l} -\Delta u+V(x)u-u\Delta(u^{2})+K(x)\phi(x)u=g(x, u),\quad x\in\mathbb {R}^{3}, \\ -\Delta\phi=K(x)u^{2}, \quad x\in\mathbb{R}^{3},\\ u\in H^{1}(\mathbb{R}^{3}),\qquad u>0, \end{array}\displaystyle \right . $$

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where V, K, g are continuous functions. To overcome the technical difficulties caused by the quasilinear term, we change the variable to guarantee the feasibility of applying the mountain pass theorem to solve the above problems. We use the mountain pass theorem and the concentration–compactness principle as basic tools to gain a nontrivial solution the system possesses under an asymptotic periodicity condition at infinity.
Improving bug report triage performance using artificial intelligence based document generation model
Authors: Lee Dong-Gun , Seo Yeong-Seok 
Published: June 2020

Abstract
Artificial intelligence is one of the key technologies for progression to the fourth industrial revolution. This technology also has a significant impact on software professionals who are continuously striving to achieve high-quality software development by fixing various types of software bugs. During the software development and maintenance stages, software bugs are the major factor that can affect the cost and time of software delivery. To efficiently fix a software bug, open bug repositories are used for identifying bug reports and for classifying and prioritizing the reports for assignment to the most appropriate software developers based on their level of interest and expertise. Owing to a lack of resources such as time and manpower, this bug report triage process is extremely important in software development. To improve the bug report triage performance, numerous studies have focused on a latent Dirichlet allocation (LDA) using the k-nearest neighbors or a support vector machine. Although the existing approaches have improved the accuracy of a bug triage, they often cause conflicts between the combined techniques and generate incorrect triage results. In this study, we propose a method for improving the bug report triage performance using multiple LDA-based topic sets by improving the LDA. The proposed method improves the existing topic sets of the LDA by building two adjunct topic sets. In our experiment, we collected bug reports from a popular bug tracking system, Bugzilla, as well as Android bug reports, to evaluate the proposed method and demonstrate the achievement of the following two goals: increase the bug report triage accuracy, and satisfy the compatibility with other state-of-the-art approaches.
How do mothers of adolescents with chronic pain experience their own quality of life?
Authors: Skarstein S. , Bergem A. K. , Helseth S. 
Published: June 2020

Abstract
Background
Chronic pain is a major health problem globally with severe personal and economic consequences. Maternal chronic pain is associated with their children’s pain. Family pain models and shared environmental aspects are important in the understanding of chronic pain among adolescents. Pain in itself impairs the quality of life (QoL). However, satisfaction in the aspects of health and functioning, social and economic, psychological, and family life will together constitute a person’s subjective experience of QoL. On this background, we considered it important to gain an understanding of the QoL of mothers who have children with chronic pain. We aimed to gain a broader understanding of the QoL in mothers of children with chronic pain and to investigate how they managed their children’s pain.

Methods
This study had a qualitative design with face-to-face, in-depth interviews. The concept of QoL was used as a framework for developing a thematic, semi-structured interview guide. Eight mothers of adolescents with chronic pain (two boys and six girls) participated with signed consent.

Results
Socio-economic difficulties and health complaints were common. Psychological stress, because of their children’s physical pain and other stressful experiences such as bullying, dominated everyday life. Lack of predictability and of responsible involvement from the fathers’ side increased the mothers’ burden considerably. Experiencing not being helped by others such as health professionals resulted in feelings of helplessness.

Conclusions
These mothers had reduced QoL caused by their own health problems, concern for the child’s well-being and lack of social support, which affected the child’s upbringing and pain management. By improving these mothers’ QoL, family-based shared pain management strategies could help in health promotion, leading to a more positive QoL circle. Elements of family and cognitive therapy could be applied when supporting the mothers and children and improving their QoL.
A randomised feasibility study of serial magnetic resonance imaging to reduce treatment times in Charcot neuroarthropathy in people with diabetes (CADOM): a protocol
Authors: Gooday Catherine , Game Frances , Woodburn Jim , Poland Fiona , Sims Erika , Dhatariya Ketan , Shepstone Lee , Hardeman Wendy 
Published: June 2020

Abstract
Background
Charcot neuroarthropathy is a complication of peripheral neuropathy associated with diabetes which most frequently affects the lower limb. It can cause fractures and dislocations within the foot, which may progress to deformity and ulceration. Recommended treatment is immobilisation and offloading, with a below knee non-removable cast or boot. Duration of treatment varies from six months to more than 1 year. Small observational studies suggest that repeated assessment with magnetic resonance imaging improves decision-making about when to stop treatment, but this has not been tested in clinical trials. This study aims to explore the feasibility of using serial magnetic resonance imaging without contrast in the monitoring of Charcot neuroarthropathy to reduce duration of immobilisation of the foot. A nested qualitative study aims to explore participants’ lived experience of Charcot neuroarthropathy and of taking part in the feasibility study.

Methods
We will undertake a two-arm, open study and randomise 60 people with a suspected or confirmed diagnosis of Charcot neuroarthropathy from five NHS, secondary care multidisciplinary Diabetic Foot Clinics across England. Participants will be randomised 1:1 to receive magnetic resonance imaging at baseline and remission up to 12 months, with repeated foot temperature measurements and X-rays (standard care plus), or standard care plus with additional three-monthly magnetic resonance imaging until remission up to 12 months (intervention). Time to confirmed remission of Charcot neuroarthropathy with off-loading treatment (days) and its variance will be used to inform sample size in a full-scale trial. We will look for opportunities to improve the protocols for monitoring techniques and the clinical, patient-centred and health economic measures used in a future study. For the nested qualitative study, we will invite a purposive sample of 10–14 people able to offer maximally varying experiences from the feasibility study to take part in semi-structured interviews to be analysed using thematic analysis.

Discussion
The study will inform the decision whether to proceed to a full-scale trial. It will also allow deeper understanding of the lived experience of Charcot neuroarthropathy, and factors that contribute to engagement in management and contribute to the development of more effective patient-centred strategies.

Trial registration
ISRCTN, ISRCTN74101606. Registered on 6 November 2017.
"Like a virgin". Correlates of virginity among Italian university students
Authors: Stranges Manuela , Vignoli Daniele 
Published: June 2020

AbstractVirginity, as a status, carries with it enormous personal and social meaning and represents a sexual milestone with high cultural significance. In this paper, we describe the correlates of virginity in Italy by consulting a large-scale survey of university students conducted in 2000 and 2017 (N = 12,400). First, we note a reduction between 2000 and 2017 of both male and female students reporting themselves as virgins at the time of the interview. Second, we highlight the salience of several demographic-, family-, interpersonal-, bodily-, and health-related factors associated with their virginity status. Third, the findings of the present study draw special attention to two crucial characteristics of Italian society: regional divisions and the role of religiosity. We show that, although religiosity is still important to the sexual lives of Italian youths, its relevance is weakening over time. In all, our study extends research on sexuality among Italian youths and sheds light on factors related to their virginity, seeking to feed future research on the topic.
Performance of trust-based governance
Authors: Vanneste Bart S. , Yoo Onesun Steve 
Published: June 2020

AbstractTrust is crucial for the success of interorganizational relationships, yet we lack a clear understanding of when trust-based governance is likely to succeed or fail. This paper explores that topic via a closed-form and a computational analysis of a formal model based on the well-known trust game. We say that trust-based governance performs better in situations where it results in a willingness to be vulnerable with trustworthy othersandan unwillingness to be vulnerable with untrustworthy others. We find that trust-based governance performs better in situations in which (a) trustworthy and untrustworthy partners exhibit markedly different behavior (high behavioral risk) or (b) the organization is willing to be vulnerable despite doubts concerning the partner’s trustworthiness (low trust threshold).
Design of high performance and low resistive loss graphene solar cells
Authors: Sabaeian Mohammad , Hajati Yaser 
Published: June 2020

AbstractDespite metallic plasmonic excitations can enhance the performance of ultra-thin solar cells however these so-called plasmonic solar cells suffer from a large resistive (Ohmic) loss caused by metallic elements. In this work, we report on a new design that uses graphene nanoribbons (GNRs) in a two-dimensional (2D) grating form at the top of the semiconductor-on-insulator (SOI) solar cells aimed to reduce the resistive loss. The results showed that GNRs can remarkably reduce the resistive loss compared to the SOI cell with Ag nanograting, while keeping all other cell’s parameters, comparable with those of Ag SOI cell. Optical absorption and short-circuit current density of the graphene cells showed, respectively, enhancements of 18 and 1.7 times when optimizations were done with respect to width and the grating period. Our calculations showed that the graphene solar cells dissipate at most 5% of incident sunlight power as narrow and tiny peaks around 508 nm, which is noticeably lower than those of Ag solar cells with high and broad band peaks with the maximum values of 29% at 480 nm and 24% at 637 nm.
Spinal mepivacaine versus bupivacaine for ultrasound guided transvaginal oocyte retrieval. A comparative study
Authors: Menshawi Mohammed Abdelsalam , Fahim Hany Magdy 
Published: June 2020

AbstractStudy objectiveThe most important limitation for spinal anesthesia in the ambulatory setting is the prolonged motor blockade and delayed postoperative ambulation. The current study was conducted to compare spinal mepivacaine–fentanyl combination versus conventional combination of spinal bupivacaine with fentanyl for surgical anesthesia of ultrasound-guided transvaginal oocyte retrieval for in vitro fertilization (IVF).Patients and methodsSixty female patients undergoing ultrasound-guided transvaginal oocyte retrieval for in vitro fertilization on ambulatory basis were enrolled in the current study. Patients were randomly distributed in to one of two equal groups: mepivacaine group (group M) and bupivacaine group (group B). Patients in group M received intrathecal 37.5 mg of isobaric mepivacaine 1.5% plus 10 μg fentanyl while patients in group B received intrathecal 12.5 mg of hyperbaric bupivacaine 0.5% plus 10 μg fentanyl. Primary outcome measure was the time to complete motor block regression. Secondary outcome measures included the peak sensory blockade level achieved, the times for sensory block regression to S1 dermatome, post-anesthesia stable ambulation, 1st voiding, and hospital discharge. The incidence of perioperative adverse events was also recorded.ResultsTwo patients (one in each group) were excluded from the study due to failed block and conversion to general anesthesia. The peak sensory blockade levels (T6–T10) were satisfactory for procedural anesthesia in the remaining patients of both groups. Patients in group M had significantly faster sensory block regression to S1 and motor block resolution when compared with group B (P< 0.05). Times to steady ambulation, voiding and hospital discharge were significantly shorter in group M when compared with group B (P< 0.05). There was no intergroup significant difference as regards the incidence of perioperative adverse events, and none of the patients reported transient neurological symptoms (TNS) or postdural puncture headache in both groups.ConclusionThe mepivacaine–fentanyl combination was superior to bupivacaine–fentanyl combination for spinal anesthesia of ultrasound-guided transvaginal oocyte retrieval on ambulatory basis because of faster sensory and motor block resolution and the shorter time to ambulation and hospital discharge with reliability of surgical anesthesia and no difference in the incidence of perioperative adverse events between both groups.