bluuebunny/crossref_metadata_2025_split huggingface dataset:
Vaginal Progesterone for Prevention of Preterm Birth in Asymptomatic High-Risk Women with a Normal Cervical Length: A Systematic Review and Meta-Analysis Protocol
Authors: Williams Kimberley Paige , McAuliffe Liam , Diacci Rosanna , Aubin Anne-Marie , Issah Ashad , Wang Carol , Phung Jason , Pennell Craig Edward 
Published: September 2020

Abstract
Background: Preterm birth (PTB) is estimated to affect 14.9 million babies globally every year. Global rates of PTB continue to increase from 9.8% to 10.6% over a 15-year period from 2000 to 2014. Vaginal progesterone is commonly used by clinicians as a prevention strategy, with recent evidence affirming the benefit of vaginal (micronized) progesterone to prevent PTB in women with a shortened cervix (<25mm). Given the low incidence of a short cervix at mid-gestation in high-risk populations further evidence is required. The objective of this review is to determine if vaginal progesterone reduces spontaneous preterm birth (sPTB) before 37 weeks in asymptomatic high-risk women with a singleton pregnancy with a normal mid-gestation cervical length. Methods: Studies will be sourced from MEDLINE and Embase databases with the search terms “progesterone” and “preterm birth”. Studies will be screened and included if they assess vaginal progesterone compared to placebo in women with a normal cervical length. The primary outcome will be sPTB <37 weeks, with secondary outcomes of sPTB <34 weeks. Two independent reviewers will conduct study screening at abstract and full text level, data extraction and risk of bias assessment with disagreements resolved by an experienced researcher. The Mantel-Haenszel statistical method and random effects analysis model will be used to produce treatment effect odds ratios and corresponding 95% confidence intervals.Discussion: This review will assess the current body of evidence and provide clarity regarding the potential benefits and best practice of use of vaginal progesterone in asymptomatic women with high risk singleton pregnancies and normal cervical length. Trial registration: This study has been registered on PROSPERO with the registration number CRD42020152051
Long-Term Outcomes of Corneal Collagen Cross-Linking: Results of 6-Years Follow-Up
Authors: Csorba Anita , Kiss Huba , Kránitz Kinga , Nagy Zoltán Zsolt 
Published: September 2020

Abstract
Background: Our aim was to evaluate the long-term effects of conventional epithelium-off corneal cross-linking performed on patients with progressive keratoconus. Methods: A retrospective analysis was performed on 34 eyes of 34 patients with progressive keratoconus underwent conventional cross-linking using Pentacam HR Scheimpflug camera. Visual acuity, spherical equivalent, keratometry, pachymetry and corneal topographic indices were analysed preoperatively and at 1, 3, and 6 years after the surgery. Results: Statistically significant decrease was detected at 1 year in spherical equivalent (p = 0.022), with no significant changes afterward (p =0.616). Uncorrected and best corrected distance visual acuity improved significantly in the first postoperative year (p =0.017 and p= 0.003, respectively), and remained stable with no significant changes at further visits (p = 0.203 and p = 0.336, respectively). Significant decrease of central corneal thickness and thinnest corneal thickness was observed at 1 year (both p <0.001), with additional thinning of thinnest corneal pachymetry up to 3 years (p = 0.01). Maximum keratometry and mean keratometry showed significant, continuous improvement between all consecutive visits over the whole follow-up period (all p-values <0.05). Significant improvement was found in the following 5 topographic indices at 1 year: ISV (p = 0.001), IVA (p = 0.028), KI (p = 0.002), CKI (p <0.001 and Rmin (p <0.001), with further improvement up to 3 years in ISV (p = 0.007), in CKI (p = 0.019) and in Rmin (p = 0.015). Rmin showed more improvement up to the end of follow-up (p <0.001).Conclusions: Conventional epithelium-off corneal cross-linking is effective in halting the progression of keratoconus and in regularization of the anterior corneal surface over a long-term follow-up period up to 6 years.
Bone Marrow Mesenchymal Stem Cells Derived miRNA-130b Enhances Epithelial Sodium Channel by Targeting PTEN
Authors: Zhang Honglei , Hong Yapeng , Liu Yanhong , Zhou Zhiyu , Ding Yan , Nie Hongguang 
Published: September 2020

Abstract
Aims: Acute lung injury (ALI) is a clinical syndrome with high morbidity and mortality, and severe pulmonary edema is one of the characteristics of ALI. Epithelial sodium channel (ENaC) located on the apical side of alveolar type 2 epithelial (AT2) cells is the primary rate limiting segments in alveolar fluid clearance. Many preclinical studies have revealed that mesenchymal stem cells (MSCs) based therapy has great therapeutic potential for ALI, while the role of ENaC in this process is rarely known. Methods: We studied the effects of bone marrow-derived MSCs (BMSCs) on the protein/mRNA expression and activity of ENaC in primary mouse AT2 and H441 cells by co-culture with them, respectively. Moreover, the changes of miRNA-130b in AT2 cells were detected by qRT-PCR, and we studied the involvement of phosphatase and tensin homolog deleted on chromosome ten (PTEN) in miRNA-130b regulated ENaC. Results: Our results demonstrated that BMSCs could increase the expression of miRNA-130b, which showed adverse effects on the protein expression of α/γ-ENaC and PTEN in AT2 cells. SiRNA-mediated downregulation of PTEN could increase the protein expression of α/γ-ENaC in AT2 cells, supporting PTEN as a negative regulator of ENaC. Conclusion: In summary, miRNA-130b in BMSCs can enhance ENaC at least partially by targeting PTEN, which may provide a promising new direction for therapeutic strategy in ALI.
FAP Beige Adipogenesis in Volumetric Muscle Loss
Authors: Wang Zili , Lee Carlin S. , He Jinshen , Jiang Kunqi , Liu Mengyao , Kim Hubert T. , Feeley Brian T. , Liu Xuhui 
Published: September 2020

Abstract
Background Volumetric muscle loss (VML) often leads to chronic muscle weakness, impaired limb function, and even permanent disability. Recent studies suggest muscle residential fibro/adipogenic progenitors (FAPs) can adopt novel beige fat differentiation promoting muscle regeneration. The goal of this study is to define the role of FAP beige adipogenesis in muscle regeneration after VML in a mouse model.Methods Three months old male C57BL/6J mice, PDGFRα-GFP reporter mice, UCP-1 reporter mice, PDGFRα-CREERT/DTA inducible FAP depletion mice and NSG immune-deficient mice were used in this study. Volumetric muscle loss (VML) was created on tibialis anterior (TA) muscle with a punch. To induce FAP beige adipogenesis, Amibegron, a beta 3 adrenergic receptor (B3AR) agonist was administered to mice with I.P. injection. In a separate group, murine and human beige adipogenic FAPs was transplanted to mouse muscle after VML. Limb function was measured with gait analysis at 2 and 6 weeks after VML. Muscle histology and FAP gene expression analysis was also conducted at 2 and 6 weeks after VML.Results After VML, we observed robust proliferation of FAPs in PDGFRα-GFP reporter mice. PDGFRα-CREERT/DTA mice inducible FAP depletion mice showed reduced muscle regeneration after FAPs are depleted, suggesting a positive role of FAP in muscle regeneration after VML. Both Amibegron treatment and beige FAP transplantation significantly improved muscle regeneration and limb function after VML.Conclusion Stimulating FAP beige adipogenesis with B3AR agonists or transplantation of beige adipogenic FAPs could serve as new strategies in treating VML.
Assessing Exposure to War-related Traumatic Events in Older Vietnamese War Survivors
Authors: Young Yvette , Korinek Kim , Zimmer Zachary , Tran Toan Khanh 
Published: September 2020

Abstract
Background: Though studies measuring war-related stressors and resultant trauma among U.S. military veterans are abundant, few studies address how wartime stressors affect military veterans native to warzones. Even fewer assess the stress exposure and resulting trauma experienced by civilians. This study aimed to develop and evaluate a measure of wartime stress exposure relevant for civilians and military veterans who survived the American War in Vietnam.Methods: The study analyzed data from a novel source, the Vietnam Health and Aging Study, which surveyed older men and women residing in central and northern Vietnam. We used a combination of exploratory and confirmatory factor analysis with posthoc tests of reliability and validity to derive measures for assessing exposure to war-related traumatic events.Results: We found that a mix of exposure to death, combat, inhospitable living conditions, and forced displacement comprises the traumatic events that potentially contribute to posttraumatic stress disorder and other mental health problems. However, the particular mix of stressful experiences constituting war trauma differs for civilians, veterans of the formal military, and former members of paramilitary organizations. Conclusions: These findings suggest the need for distinct, but parallel approaches to measuring war-related stressors for populations of veterans and civilians exposed to war in their home countries, and the need for greater public attention to the potential lingering trauma of noncombatants.
DA-EPOCH-R is more effective and less toxic than modified CODOX-M/IVAC-R for treating Chinese HIV-infected patients with Burkitt lymphoma: an uncontrolled retrospective clinical trial
Authors: Ding Xiaoyan , Sun Wei , Shen Yanjun , Guo Xiaodi , Teng Ying , Liu Xiaomin , Li Wendong , Chen Jinglong , Li Wei 
Published: September 2020

Abstract
BACKGROUND Burkitt lymphoma (BL), commonly associated with human immuno-deficiency virus (HIV) in China, is an aggressive B-cell lymphoma with a poor prognosis. Current treatments are insufficient and have severe side effects in adult patients with immunodeficiency. METHODS We retrospectively studied HIV-positive patients with untreated BL from December 2011 to June 2019. We compared a low-intensity treatment regimen comprising infused etoposide, doxorubicin, and cyclophosphamide with vincristine, prednisone, and rituximab (dose-adjusted EPOCH-R, study group) and a dose-intensive regimen comprising cyclophosphamide, vincristine, doxorubicin, high-dose methotrexate/ifosfamide, etoposide, high-dose cytarabine, and rituximab (CODOX-M/IVAC-R, control group). Differences in survival outcomes, toxicity, and survival-associated factors were analyzed.RESULTS Twenty-eight consecutive patients were enrolled with integrated clinical data, with 18 patients in the DA-EPOCH-R group and 10 in the modified CODOX-M/IVAC-R group. The median age of patients was 40 years (range: 21–60 years). The median baseline absolute CD4+ cell count was 243.5 cells/µL. All patients had high-risk diseases. Overall, grade 3–4 toxicity was observed at least once in 22 patients. The principal grade 3–4 toxic events, namely leukopenia (100% vs. 50%), febrile neutropenia (90% vs. 22.2%), and thrombocytopenia (80% vs. 16.7%), were significantly higher in the control group than in the study group. One patient in the control group died of septic shock induced by pneumonia. The median follow-up time was 19 months, and the median overall survival (OS) was significantly improved in the study group compared with that in the control group [18.0 months (95% confidence interval [CI], 14.0–22.0 months) vs. 7.9 months (95% CI, 4.8–11.0 months), respectively; P = 0.032]. Treatment with DA-R-EPOCH was a favorable prognostic factor for OS (HR: 0.19 [95% CI: 0.05–0.68]; P = 0.011).CONCLUSIONS DA-EPOCH-R may be an alternative to modified CODOX-M/IVAC-R, with better efficacy and lower toxicity, for treating Chinese patients with high-risk HIV-associated BL.
Ultrahigh ionic conductivity in optimally sintered Li10.35Ge1.35P1.65S12 superionic conductor
Authors: Wen Hao , Jiang Yue , Liu Xingang , Zhu Xiaohong , Zhang Chuhong 
Published: September 2020

Abstract
Lithium superionic conductor Li10+δGe1+δP2−δS12 has attracted tremendous interest for advanced all-solid-state lithium ion batteries due to extremely high ionic conductivity. However, the synthetic processes reported in literature are widely divergent, resulting in an order of magnitude difference in ionic conductivities of the same material, but as far as we know, the influence of synthetic conditions on ionic conductivity has not been studied yet. Herein, we systematically investigate the influence of sintering temperature on phase composition and ionic conductivity of the Li10+δGe1+δP2−δS12 compounds synthesized by conventional solid-state reaction for the first time. It is found that low and high sintering temperatures lead to a low crystallinity and the formation of impurity phases, respectively. As a result, the pure Li10.35Ge1.35P1.65S12, well crystallized in space group P42/nmc, is fabricated by optimization of the solid-state reaction temperature at 580 °C and its room temperature conductivity (19 mS cm− 1) is the highest among all existing Li10+δGe1+δP2−δS12 solid electrolytes. Meanwhile, the microstructure of Li10.35Ge1.35P1.65S12, being very dense and uniform, is demonstrated firstly by atomic force microscopy.
Serum Amyloid A levels and α2- and Gamma globulins on Serum Protein Electrophoresis in cats exposed to and infected with Leishmania infantum
Authors: Savioli Giulia , Archer Joy , Brianti Emanuele , Schnyder Manuela , Iatta Roberta , Otranto Domenico , Cantacessi Cinzia 
Published: September 2020

Abstract
Background: Although dogs are the main reservoir host of Leishmania infantum, recent investigations indicate a role for cats in its epidemiology. Feline leishmaniosis (FeL) remains poorly characterised, partly due to the lack of diagnostic tools. This study aimed to compare Serum Amyloid A (SAA) levels and serum protein electrophoresis (SPE) profiles (specifically, α2- and gamma globulins) in cats naturally exposed to or infected by L. infantum from southern Italy with those of healthy controls and of cats with neoplastic or inflammatory conditions from non-endemic areas.Methods: Serum or plasma samples from four cohorts of cats were analysed for SAA and by SPE, i.e G1: healthy controls from Leishmania-non-endemic regions of Switzerland, G2: cats pre-diagnosed with neoplastic or inflammatory conditions from the University of Cambridge’s sample archive, G3: L. infantum seropositive, qPCR-negative cats from southern Italy, G4: L. infantum seropositive and qPCR-positive cats from southern Italy. SAA data was assessed for normality using the Kolmogorov-Smirnoff and Shapiro-Wilk normality tests, then compared using a homogeneity of variance test for non-parametric data; the Kruskall-Wallis test, followed by Dunn’s multiple comparison test were used to compare SAA serum levels between groups. The Fisher’s Exact test was used to assess statistically significant differences in the numbers of animals displaying elevated gamma globulins and increased α2-globulins between groups.Results: Overall, 68 samples were analysed (G1 n=16, G2 n=20, G3 n=20, G4 n=12). Cats previously exposed to and/or infected with L. infantum, as well as cats suffering from neoplastic and inflammatory conditions showed significantly higher SAA levels (median values G1=0.00 (0.00-0.00) mg/L, G2=0.85 (0.00-49.55) mg/L, G3=0.00 (0.00-4.53) mg/L, G4= 0.00 (0.00-7.5) mg/L), and higher percentages of cats with increased α2-globulins (G1=20.0% ±10.3, G2=80.0% ±8.9, G3=70.0% ±10.2, G4=75.0% ±12.5) and gamma globulins (G1=0.0% ±0, G2=65.0% ±10.7, G3=50.0% ±11.2, G4=58.3% ±14.2) than healthy control cats. For all three markers, there was no significant difference between G2, G3 and G4.Conclusions: This study indicates that, whilst levels of gamma and α2-globulins and SAA are significantly elevated in cats infected by L. infantum, they cannot be used to differentiate between L. infantum infection or exposure and neoplastic or inflammatory conditions. Nevertheless, these indicators might assist monitoring of ongoing FeL if further studies indicate reduction during or following successful treatment.
WITHDRAWN: Examining Substance Use Services and Treatment Barriers and Needs among People with Lived Experience (PWLE) in Ontario, Canada.
Authors: Russell Cayley , Ali Farihah , Nafeh Frishta , LeBlanc Sean , Imtiaz Sameer , Elton-Marshall Tara , Rehm Jürgen 
Published: September 2020

Abstract
Introduction: Substance use is a complex issue, with rates of illicit and licit substances varying across Canada, and in Ontario, specifically. Services and treatment options for problematic substance use remain vital. Recent initiatives to increase the effectiveness of services have been implemented, however, a disconnect remains between the availability and accessibility of these programs and the real-world experiences and needs of people with lived experience (PWLE). There is a lack of knowledge regarding barriers to accessing services and service needs, yet PWLE are best suited to identify these factors. As such, this study critically examined these issues among a cohort of PWLE in Ontario, Canada. Methods: Semi-structured, one-on-one interviews with n=50 adult PWLE participants were conducted. Participants were recruited from substance use services across Ontario, based on ConnexOntario’s directory of all provincial addiction services, as well as by word-of-mouth. Questions focused on participants’ experiences and perspectives on substance use services. All data were transcribed and underwent an inductive thematic analysis based on key themes that emerged. Results: Beneficial services identified varied based on participant experiences and needs. A variety of barriers to service access were acknowledged, primarily related to system-level issues such as an overall lack of services, a lack of service capacity, and a severe lack of service coordination and gaps in service delivery. Recommendations revolved around the need for low-barrier, affordable, seamless services run by non-judgemental staff, including fellow PWLE. Conclusions: This study identified needs and barriers to substance use care among PWLE in Ontario, and highlighted important areas for policy change and program planning and implementation. Concrete recommendations include the development of a government-funded, low-barrier, comprehensive and integrated model of substance use services that includes PWLE as collaborators and program facilitators to ensure that services are as successful and meaningful as possible. Results from this study will work towards the improvement of substance use treatment and service provision across the province.
CircINSR regulates fetal bovine muscle and fat development
Authors: Shen Xuemei , Tang Jia , Ru Wenxiu , Zhang Xiaoyan , Huang Yongzhen , Lan Xianyong , Lei Chuzhao , Cao Hui , Chen Hong 
Published: September 2020

Abstract
Background: The level of muscle development directly affects the production efficiency of livestock, and the content of intramuscular fat (IMF) is an important factor affecting meat quality. Nevertheless, the molecular mechanism of embryonic circular RNA in muscle and IMF development remains largely unknown. Methods: In this study, we isolated myoblasts and intramuscular preadipocytes from fetal bovine skeletal muscle. Oil Red O and BODIPY staining were used to identify lipid droplets of preadipocytes, and anti-MyHC immunofluorescence was used to identify myotubes differentiated from myoblasts. Bioinformatics, dual fluorescence reporter system, and RNA immunoprecipitation were used to determine the interactions between circINSR and miR-15/16 family. Molecular and biochemical assays were used to confirm the role of circINSR in myoblasts and intramuscular preadipocytes. Results: We found that the isolated myoblasts and preadipocytes can differentiate normally. Besides, circINSR severed as a sponge of miR-15/16 family, which targeting CyclinD1 and Bcl-2. CircINSR overexpression significantly promoted myoblasts and preadipocytes proliferation, and inhibited cell apoptosis. In addition, circINSR inhibited preadipocytes adipogenesis by alleviating the inhibition of miR-15/16 on target genes FOXO1 and EPT1. Conclusions: Taken together, our study demonstrated circINSR as a regulator of embryonic muscle and IMF development.
Inverse molecular docking reveals a novel function of thymol: inhibition of fat deposition induced by high-dose glucose in Caenorhabditis elegans
Authors: Ban Fangfang , Hu Liangbin , Zhou Xiaohui , Zhao Yanyan , Mo Haizhen , Li Hongbo , Wei Zhou 
Published: September 2020

Abstract
Background As a natural product isolated from thyme oil in thyme, thymol (2-isopropyl-5-methylphenol) harbors antiviral, antioxidant, and other properties, and thus could be potentially used for the treatment of various diseases. However, the function of thymol has not been comprehensively studied.Methods Here, we applied an inverse molecular docking approach to identify unappreciated functions of thymol. Potential targets of thymol in humans were identiﬁed by the server of DRAR-CPI, and targets of interest were then assessed by GO and KEGG pathway analysis. Subsequently, homologous proteins of these targets in Caenorhabditis elegans were identified by Blast tool and their three-dimensional structures were achieved using Swiss-Model workspace. Interaction between thymol and the targeted proteins in worms was verified using AutoDock 4.0. To verify the activity of thymol on lipid deposition in vivo, the C. elegans model was established. The lipid content of nematodes induced by high-dose glucose was determined by Oil Red O and Nile Red staining, and gene expression related to fat accumulation was assessed by qRT-PCR. Results Analyses of the targets revealed that thymol could be potentially involved in the glycolysis/gluconeogenesis and fatty acid degradation pathways. Experiment results in vivo verified that 1 μg/mL and 5 μg/mL thymol treatment significantly reduced lipid deposition in glucose-induced nematodes. The result of qRT-PCR suggested that the decreased level of cpt-1, aco, fabp and tph-1 correlating with β-oxidation in nematodes exposed by 1mM glucose were upregulated after the exposure of thymol.Conclusions The results showed that thymol might lead to the acceleration of β-oxidation by upregulating cpt-1, aco, fabp and tph-1, causing the descent of lipid content in nematodes, which is potentially used for the treatment of chronic metabolic diseases associated with increased fatty acid deposition.
Canthin-6-One 9-O-Beta-Glucopyranoside: an inhibitor of SARS-CoV-2 (COVID 19) proteases PLpro and Mpro/ 3CLpro
Authors: Verma Devvret , Mitra Debasis , Kamboj Anshul , Mahakur Bhaswatimayee , Chaudhary Priya , Shrivastav Rakesh , Janmeda Pracheta , Pant Kumud , Mohapatra Pradeep K. Das 
Published: September 2020

Abstract
The rapid spread of SARS-CoV-2 has raised a severe global public health issue, where therapy is not identified with specific drugs or vaccines. The present investigation dealt with the inhibition of various proteins and receptors of virus using phytocompounds of three pertinent medicinal plants i.e. Eurycoma harmandiana, Sophora flavescens and Andrographis paniculata. Phytocompounds known to have antiviral properties were screened against the papain-like protease (PLpro) and main protease (Mpro) / 3-chymotrypsin-like Protease (3CLpro). Molecular docking with Canthin-6-One 9-O-Beta-Glucopyranoside showed the highest binding affinity and least binding energy with both the proteases viz. PLpro and Mpro/ 3CLpro. ADMET analysis of the compound suggested that it is having drug-like properties like high gastrointestinal (gi-) absorption, no blood-brain barrier permeability, and high lipophilicity.