bluuebunny/crossref_metadata_2025_split huggingface dataset:
Knowledge of prevention of mother to child transmission of HIV among women of reproductive age group and associated factors at Mecha district, Northwest Ethiopia
Authors: liyeh Tewachew Muche , Cherkose Endeshaw Admasu , Limenih Miteku Andualem , Yimer Tigist Seid , Tebeje Hailemariam Demewozu 
Published: January 2020

Abstract
Objective: This study was aimed to asses knowledge of prevention of mother to child transmission of HIV among reproductive age women and associated factors at Mecha district, North West Ethiopia. A community based cross sectional study was conducted among 853 reproductive age women from July 1- 30/2016 in Mecha district. By multistage sampling technique data were collected through pre-tested questionnaire. The collected data was entered in Epi Data 3.1 and analyzed with SPSS version 20. Bivariate and multivariable logistic regression model were used. Result: About 22.4% of the respondents were knowledgeable on prevention of mother to child transmission (PMTCT) of HIV. Having knowledge on PMTCT of HIV was significantly associated with urban residence (AOR =2.486, 95%CI= 1.160-5.328), education level of secondary and above (AOR =5.445, 95%CI=2.698-10.986), those having history of antenatal care followup (AOR =4.430, 95%CI=1.471-13.340), those with history of institutional delivery (AOR=4.766, 95%CI = 2.004-11.334), those having comprehensive knowledge on HIV/AIDS (AOR=1.697, 95%CI = 1.011-2.846), women who were knowledgeable about mother to child transmission of HIV (AOR =2.203, 95% CI =1.37-3.54), and women who held discussions with their husband regarding HIV/AIDS, (AOR= 2.700, 95%CI =1.658-4.396). Key words: knowledge, PMTCT, HIV, Women, Ethiopia
Characterisation of the gut microbial community of rhesus macaques in high-altitude environments
Authors: Wu Yuhan , Yao Yongfang , Dong Mengmeng , Xia Tianrui , Li Diyan , Xie Meng , Wu Jiayun , Wen Anxiang , Wang Qin , Zhu Guangxiang , Ni Qingyong , Zhang Mingwang , Xu Huailiang 
Published: January 2020

Abstract
Background: The mammal intestinal microbiota is involved in various physiological processes and plays a key role in host environment adaption. However, for non-human primates (NHPs), little is known about their gut microbial community in high-altitude environments and even less about their adaption to such habitats. We characterised the gut microbial community of rhesus macaques from multiple high-altitude environments and compared it to those of low-altitude populations. Results: We collected faecal samples of rhesus macaques from four high-altitude populations (above 3,000 m) and three low-altitude populations (below 500 m). By calculating the alpha diversity index, we found that high-altitude populations exhibited a higher diversity. Statistical analysis of beta diversity indicated significant differences between high- and low-altitude populations. Significant differences were also detected at the phylum and family levels. At the phylum level, the high-altitude gut microbial community was dominated by Firmicutes (63.42%), while at low altitudes, it was dominated by Bacteroidetes (47.4%). At the family level, the high-altitude population was dominated by Ruminococcaceae (36.2%), while the low-altitude one was dominated by Prevotellaceae (39.6%). Some families, such as Christensenellaceae, were consistently higher abundant in all high-altitude populations. We analysed the overlap of operational taxonomic units (OTUs) in high-altitude populations and determined their core OTUs (shared by all four high-altitude populations). However, when compared with the low-altitude core OTUs, only 65% were shared, suggesting a divergence in core OTUs. Function prediction indicated a significant difference in gene copy number of 35 level-2 pathways between high- and low-altitude populations; 29 of them were higher in high altitudes, especially in membrane transport and carbohydrate metabolism. Conclusions: The gut microbial community of high-altitude rhesus macaques was significantly distinct from that of low-altitude populations in terms of diversity, composition and function. High-altitude populations were dominated by Firmicutes and Ruminococcace, while in low-altitude populations, Bacteroidetes and Prevotellaceae were dominant. The difference in gut microbiota between these two populations may be caused by differences in host diet, environmental temperature and oxygen pressure. These gut microbial microorganisms may play a critical role in the adaptive evolution of rhesus macaques to high-altitude environments.
Surgical Workflow Simulation for the Design and Assessment of Operating Room Setups in Orthopedic Surgery
Authors: Neumann Juliane , Angrick Christine , Höhn Celina , Zajonz Dirk , Ghanem Mohamed , Roth Andreas , Neumuth Thomas 
Published: January 2020

Abstract
Background The design and internal layout of modern operating rooms are influencing the team’s collaboration and communication, ergonomics, as well as intraoperative hygiene substantially. Nevertheless, there is no objective method for the assessment and design of the personnel and table positions for different surgical disciplines and intervention types available. The aim of this work is to establish an improved OR setup for common procedures in arthroplasty. Methods With the help of computer simulation techniques, a method for the objective design and assessment of enhanced OR setups was developed. In this work, new OR setups were designed, analyzed in a computer simulation environment and evaluated in the actual intraoperative setting. Results The implementation of improved OR setups reduces the instrument handover time between the surgeon and the scrub nurse, the travel paths of the OR team as well as shortens the procedure duration. Additionally, the ergonomics of the OR staff were improved. Conclusion The developed simulation method was intraoperatively evaluated and proved its benefit for the design and optimization of OR setups for different surgical intervention types. As a clinical result, enhanced setups for total knee arthroplasty and total hip arthroplasty surgeries were established in daily clinical routine and the OR efficiency was improved.
Yarning about oral health: perceptions of urban Australian Aboriginal and Torres Strait Islander Women
Authors: Butten Kaley , Johnson Newell W. , Hall Kerry K. , Toombs Maree , King Neil , O’Grady Kerry-Ann F. 
Published: January 2020

Abstract
Background: Many factors influence how a person experiences oral health and how such experiences may facilitate supportive oral health behaviours. Women in particular face different challenges due to their environment, responsibilities and physiological differences to men. Within Australia, Aboriginal and Torres Strait Islander women are reported to have poorer oral health and are faced with additional barriers to supporting their oral health compared with non-Indigenous women. The objective of this paper is to report the experiences and perceptions of oral health from the perspective of urban, Aboriginal and Torres Strait Islander women. Methods: The present data derive from a descriptive study that used yarning circles and face-to-face interviews with women who were mothers/carers of urban, Aboriginal and/or Torres Strait Islander children. This was a qualitative study to investigate the impact of child oral health on families. Participants used the opportunity to share their own personal experiences of oral health as women, thus providing data for the present analyses. Information collected was transcribed and analysed thematically Results: 20 women shared their personal narratives on the topic of oral health which were reflective of different time points in their life: growing up, as an adult and as a mother/carer. Although women are trying to support their oral health across their life-course, they face a number of barriers, including a lack of information and the costs of accessing dental care. The teenage years and pregnancy were reported as important time periods for oral health support. Conclusions: To improve the oral health of Indigenous Australian women, policymakers must consider the barriers reported by women and critically review current oral health information and services. Current oral health services are financially out of reach for Indigenous Australian women and there is not sufficient or appropriate, oral information across the life-course.
Carbonate dual-phase improves the performance of single-layer fuel cell made from mixed ionic and semiconductor composite
Authors: Jouttijärvi S. , Yao Xueli , Asghar M. I. , Etula Jarkko , Reinecke A.-M. , Lippmann W. , Lund Peter 
Published: January 2020

AbstractA mixed ionic and semiconducting composite in a single-layer configuration has been shown to work as a fuel cell at a lower temperature (500-600 oC) than a traditional solid-oxide fuel cell. The performance of a single-layer fuel cell (SLFC) is often limited by high resistive losses. Here, an eutectic mixture of alkali-carbonates was added to SLFC to improve the ionic conductivity. The dual-phase composite ionic conductor consisted of a ternary carbonate (sodium lithium potassium carbonate, NLKC) mixed with gadolinium-doped cerium oxide (GDC). Lithium nickel zinc oxide (LNZ) was used as the semiconducting material. The LNZ-GDC-NLKC SLFC reached a high power density, 582 mW/cm2 (conductivity 0.22 S/cm) at 600 °C, which is more than 30 times better than without the carbonate. The best results were obtained with the ternary carbonate which decreased the ohmic losses of the cell by more than 95%, whereas the SLFC with a binary carbonate (sodium lithium carbonate, NLC) showed a lower conductivity and performance (243 mW/cm2, 0.17 S/cm at 600°C). It is concluded that adding carbonates to LNZ-GDC will improve the ionic conductivity and positively contribute to the cell performance. These results suggest a potential path for further development of SLFCs, but also imply the need for efforts on up-scaling and stability to produce practical applications with SLFC.
High-Throughput Sequencing of Virus-infected Cucurbita pepo Samples Revealed The Presence of Zucchini Shoestring Virus in Zimbabwe
Authors: Karavina Charles , Ibaba Jacques Davy , Gubba Augustine 
Published: January 2020

Abstract
Objectives: Plant-infecting viruses remain a serious challenge towards achieving food security worldwide. Cucurbit virus surveys were conducted in Zimbabwe during the 2014 and 2015 growing seasons. Leaf samples displaying virus-like symptoms were collected and stored until analysis. Three baby marrow samples were subjected to next-generation sequencing and the data generated were analysed using genomics technologies. Zucchini shoestring virus (ZSSV), a cucurbit-infecting potyvirus previously described in South Africa was one of the viruses identified. The genomes of the three ZSSV isolates are described analysed in this note. Results: The three ZSSV isolates had the same genome size of 10297 bp excluding the polyA tail with a 43% GC content. The large open reading frame was found at positions 69 to 10106 on the genome and encodes a 3345 amino acids long polyprotein which had the same cleavage site sequences as those described on the South African isolate except for the P1-pro site. Genome sequence comparisons of all the ZSSV isolates showed that the isolates F7-Art and S6-Prime had identical sequence across the entire genome while sharing 99.06% and 99.34% polyprotein nucleotide and amino acid sequence identities respectively with the isolate S7-Prime.
Association among biofilm formation, virulence gene expression, and antibiotic resistance in Proteus mirabilis isolates from diarrhetic animals in northeast China
Authors: Sun Yadong , Wen Shanshan , Zhao Lili , Xia Qiqi , Pan Yue , Liu Hanghang , Wei Chengwei , Chen Hongyan , Ge Junwei , Wang Hongbin 
Published: January 2020

Abstract
Background The aim of this study was to investigate the association among biofilm formation, virulence gene expression, and antibiotic resistance in P. mirabilis isolates collected from diarrhetic animals (n = 176) in northeast China between September 2014 and October 2016. Results Approximately 92.05% of the isolates were biofilm producers, whereas 7.95% of the isolates were non-producers. The prevalence of virulence genes in biofilm producers was significantly higher than that in non-producers. Biofilm production was significantly associated with the expression of ureC , zapA , rsmA , hmpA , mrpA , atfA , and pmfA ( P < 0.05). Drug susceptibility tests revealed that approximately 76.7% of the isolates were multidrug-resistant (MDR) and extensively drug-resistant (XDR). Biofilm production was significantly associated with resistance to doxycycline, tetracycline, sulfamethoxazole, kanamycin, and cephalothin ( P < 0.05). Although the pathogenicity of the biofilm producers was stronger than that of the non-producers, the biofilm-forming ability of the isolates was not significantly associated with morbidity and mortality in mice ( P > 0.05). Conclusion Our findings suggested that a high level of multidrug resistance in diarrhetic animals infected with P. mirabilis in northeast China.The results of this study indicated that the positive rates of the genes expressed by biofilm-producing P. mirabilis isolates were significantly higher than those expressed by non-producing isolates.
A scoping review of initiatives to reduce inappropriate or non-beneficial hospital admission and bed days in people nearing the end of their life: Much innovation but limited supporting evidence
Authors: Taylor Ros , Ellis Jonathan , Gao Wei , Searle Liz , Heaps Kate , Davies Robert , Hawksworth Claire , Garcia-Perez Angela , Colclough Giles , Walker Steven , Wee Bee 
Published: January 2020

AbstractObjectives Hospitalisation during the last weeks of life when there is no medical need or desire to be there is distressing and expensive. This study sought palliative care initiatives which may avoid or shorten hospital stay at the end of life and analysed their success in terms reducing bed days. Methods Part 1 included a search of literature in PubMed and Google Scholar between 2013 and 2018, an examination of governmental and organisational publications plus discussions with external and co-author experts regarding other sources. This initial sweep sought to identify and categorise relevant palliative care initiatives. In Part 2, we looked for publications providing data on hospital admissions and bed days for each category. Results A total of 1252 abstracts were reviewed, resulting in ten broad classes being identified. Further screening revealed 50 relevant publications describing a range of multi-component initiatives. Studies were generally small and retrospective. Most researchers claim their service delivered benefits. In descending frequency, benefits identified were support in the community, integrated care, out-of-hours telephone advice, care home education and telemedicine. Nurses and hospices were central to many initiatives. Barriers and factors underpinning success were rarely addressed. Conclusions A wide range of initiatives have been introduced to improve end-of-life experiences. Formal evidence supporting their effectiveness in reducing inappropriate/non-beneficial hospital bed days was generally limited or absent.
Prevalence of Group B Streptococcus Colonization in Pregnant Women in Jiangsu, East China
Authors: Wang Rui , Qiu Hongmei , Yanmei Ge , Pan Fei , Bian Shuhui 
Published: January 2020

Abstract
Background: Group B streptococcus (GBS) is the leading cause of early-onset neonatal sepsis. However, GBS was infrequently reported in the developing world in contrast to western countries. This study assessed the prevalence of GBS colonization among pregnant women in Jiangsu, East China and reveled the difference of GBS infection between culture and PCR .
Methods: A total of 16,184 pregnant women at 34 to 37 weeks, gestation aged 16–47 years were recruited from Nanjing Kingmed Diagnostics. There were 9022 pregnant women receiving GBS screening by PCR detection only. 7162 pregnant women received GBS screening by bacterial culture and GBS-positive samples were tested for antibiotic resistance.
Results: The overall GBS positive rate was 8.7% by PCR and 3.5% by culture. There was no significant age difference of GBS infection, but the 25-29 age group and people aged over 40 years should pay more attention . The 249 GBS-positive samples which detected by culture were all sensitive to penicillin. The prevalence of resistance to erythromycin, clindamycin and levofloxacin was 77.5%, 68.3% and 52.2%, respectively.
Conclusions: This study revealed the data on the prevalence of GBS colonization in pregnant women in Jiangsu, East China. And it compared the difference of GBS infection between culture and PCR. PCR was expectd to become a quick method in pregnancy women conventional detection of GBS infection.
Extracellular vesicle-mediated amyloid transfer to neural progenitor cells: implications for RAGE and HIV infection
Authors: András Ibolya E. , Garcia-Contreras Marta , Yanick Christopher , Perez Paola , Sewell Brice , Durand Leonardo , Toborek Michal 
Published: January 2020

Abstract
Amyloid beta (Aβ) deposition was demonstrated to be elevated in the brains of HIV-infected patients and associated with neurocognitive decline; however, the mechanisms of these processes are poorly understood. The goal of the current study was to address the hypothesis that Aβ can be transferred via extracellular vesicles (ECVs) from brain endothelial cells to neural progenitor cells (NPCs) and that this process can contribute to abnormal NPC differentiation. Mechanistically, we focused on the role of the receptor for advanced glycation endproducts (RAGE) and activation of the inflammasome in these events. ECVs loaded with Aβ (Aβ-ECVs) were readily taken up by NPCs and Aβ partly colocalized with the inflammasome markers ASC and NLRP3 in the nuclei of the recipient NPCs. This colocalization was affected by HIV and RAGE inhibition by a high-affinity specific inhibitor FPS-ZM1. Blocking RAGE resulted also in an increase in ECV number produced by brain endothelial cells, decreased Aβ content in ECVs, and diminished Aβ-ECVs transfer to NPC nuclei. Interestingly, both Aβ-ECVs and RAGE inhibition altered NPC differentiation. Overall, these data indicate that RAGE inhibition affects brain endothelial ECV release and Aβ-ECVs transfer to NPCs. These events may modulate ECV-mediated amyloid pathology in the HIV-infected brain and contribute to the development of HIV-associated neurocognitive disorders.
Role of school as supportive entity for positive oral health behaviour of adolescents in south-western Nigeria
Authors: Lawal Folake Barakat , Oke Gbemisola Aderemi 
Published: January 2020

Abstract
Background The school is primed as an avenue for promoting health among adolescents. Conversely, the high unmet dental needs among school-going adolescents raises concern about the role of schools in this task. This study therefore assessed the role of schools in promoting positive oral health behaviour among adolescents through Capabilities, Opportunities, Motivation (COM-B) model in a Low-Income-Country.Methods Two thousand and ninety-seven students aged 12-18 years were recruited from 30 randomly selected Secondary Schools in a cross sectional study conducted in Ibadan, Nigeria. Data on Capabilities; Oral Health Knowledge (K), Attitude (A), Practices (P) and Motivation of the students as well as oral health promotional (OHP) activities (Opportunities) in the schools were assessed through a questionnaire. A checklist was used to assess availability of OHP materials and the schools’ tuck shops for sale of healthy food (Opportunities). Data were analyzed with STATA version 14.Results The mean KAP score was 43.8 (± 11.4)%. Oral health education was the only activity reported and this was in 8 (26.7%) schools; only 331 (15.8%) students had been educated about their oral health. There were no oral health promotional materials in the schools. About half 1161 (55.4%) were motivated to participate in school oral health program. All the tucks shops had cariogenic foods and drinks for sale. Students who had better KAP scores - Capabilities (OR = 1.2, 95%CI = 1.0-1.5, p = 0.018) or had been educated about oral health - Opportunities (OR = 1.5, 95%CI = 1.2-2.0, p < 0.001) were better motivated about their oral health.Conclusion The schools surveyed played very little or no role to support positive oral health behavior of adolescents. There were no OHP materials in the schools. Students who had superior oral health KAP (capabilities) or had been exposed to oral health education (opportunities) were better motivated about their oral health.
A Retrospective study of Low vision intervention in individuals with central field loss and peripheral field loss
Authors: Raman Rajiv , Gopalakrishnan Sarika , Sathyaprasath Mathangi 
Published: January 2020

Abstract
Background The objective of this study was to estimate the visual impairment in patients with central field loss (CFL) and peripheral field loss (PFL), to analyze the type of low vision devices prescribed and to compare the visual benefits who presented to Low Vision Care clinic at a tertiary eye care center in India. Methods A retrospective review was done for 450 patients with low vision who visited LVC clinic from January 2017 to December 2017. The subjects were categorized into two groups: CFL and PFL. Demographic profile such as age, gender, occupation, ocular history, visual acuity status and low vision device preferred was collected and documented. Results Out of 450 patients, 242 (53.8%) were diagnosed to have CFL and 208 (46.2%) had PFL. 323 (71.8%) were men and 127 (28.2%) were women. The sum of median age of both the groups were 34.5 years, while the median age of CFL and PFL group was 36 and 34 years respectively. Atrophy related macular disorder (54.1%) was the major reason causing central field loss and Retinitis Pigmentosa (81.7%) for peripheral field loss. CFL group (76%) preferred low vision devices more than PFL group (65.9%). The ratio of task difficulties for both distance and near were 1:2 for central and peripheral field loss respectively. Electronic video magnifier was preferred more in CFL group. There was a statistically significant improvement ( P < 0.05) in distance and near vision with the help of low vision devices in both the groups. ConclusionsThe use of low vision devices in both CFL and PFL can help the patients in restoring useful vision.