bluuebunny/crossref_metadata_2025_split huggingface dataset:
CCND1, NOP14 and DNMT3B are involved in miR‐502‐5p–mediated inhibition of cell migration and proliferation in bladder cancer
Authors: Ying Yufan , Li Jiangfeng , Xie Haiyun , Yan Huaqing , Jin Ke , He Liujia , Ma Xueyou , Wu Jian , Xu Xin , Fang Jiajie , Wang Xiao , Zheng Xiangyi , Liu Ben , Xie Liping 
Published: January 2020

AbstractObjectivesDownregulation of miR‐502‐5p has emerged as a critical factor in tumour progression in several cancers. Herein, we elucidated the role of miR‐502‐5p in bladder cancer.Materials and methodsRT‐qPCR was performed to examine the expression of miR‐502‐5p in bladder cancer. And DNA methylation analysis showed that epigenetic mechanisms may contribute to the downregulation of miR‐502‐5p. Then, wound‐healing assay, transwell assay, colony formation assay, CCK8 assay and flow cytometry analysis were applied to evaluate the function of miR‐502‐5p in bladder cancer cell lines. Western blot was conducted to measure the protein levels of related genes. Furthermore, dual‐luciferase reporter assay, in vivo tumorigenesis assay and immunohistochemical staining were also conducted as needed.ResultsMiR‐502‐5p is frequently downregulated in BCa. Meanwhile, hypermethylation of CpG islands contributes to the downregulation of miR‐502‐5p. Functionally, overexpression of miR‐502‐5p inhibited cell proliferation and migration in vitro and repressed tumour growth in vivo. CCND1, DNMT3B and NOP14 were identified as direct targets of miR‐502‐5p. Interestingly, DNMT3B and miR‐502‐5p established a positive feedback loop in the regulation of bladder cancer. In addition, rescue experiments further validated the direct molecular interaction between miR‐502‐5p and its targets.ConclusionsOur study proposed and demonstrated that the miR‐502‐5p–mediated regulatory network is critical in bladder cancer; this network may be useful in the development of more effective therapies against bladder cancer.
Reducing the burden of hypoglycaemia in people with diabetes through increased understanding: design of the Hypoglycaemia REdefining SOLutions for better liVEs (Hypo‐RESOLVE) project
Authors: de Galan B. E. , McCrimmon R. J. , Ibberson M. , Heller S. R. , Choudhary P. , Pouwer F. , Speight J. , Carlton J. , Pieber T. R. , Rosilio M. , Tack C. J. , Müllenborn M. ,   for the Hypo‐RESOLVE Consortium
Published: January 2020

AbstractBackgroundHypoglycaemia is the most frequent complication of treatment with insulin or insulin secretagogues in people with diabetes. Severe hypoglycaemia, i.e. an event requiring external help because of cognitive dysfunction, is associated with a higher risk of adverse cardiovascular outcomes and all‐cause mortality, but underlying mechanism(s) are poorly understood. There is also a gap in the understanding of the clinical, psychological and health economic impact of ‘non‐severe’ hypoglycaemia and the glucose level below which hypoglycaemia causes harm.AimTo increase understanding of hypoglycaemia by addressing the above issues over a 4‐year period.MethodsHypo‐RESOLVE is structured across eight work packages, each with a distinct focus. We will construct a large, sustainable database including hypoglycaemia data from >100 clinical trials to examine predictors of hypoglycaemia and establish glucose threshold(s) below which hypoglycaemia constitutes a risk for adverse biomedical and psychological outcomes, and increases healthcare costs. We will also investigate the mechanism(s) underlying the antecedents and consequences of hypoglycaemia, the significance of glucose sensor‐detected hypoglycaemia, the impact of hypoglycaemia in families, and the costs of hypoglycaemia for healthcare systems.ResultsThe outcomes of Hypo‐RESOLVE will inform evidence‐based definitions regarding the classification of hypoglycaemia in diabetes for use in daily clinical practice, future clinical trials and as a benchmark for comparing glucose‐lowering interventions and strategies across trials. Stakeholders will be engaged to achieve broadly adopted agreement.ConclusionHypo‐RESOLVE will advance our understanding and refine the classification of hypoglycaemia, with the ultimate aim being to alleviate the burden and consequences of hypoglycaemia in people with diabetes.
Human Amniotic Membrane: A New Option for Graft Donor Sites – Systematic Review and Meta‐analysis
Authors: Abul Ahmad , Karam Mohammad , Rahman Shafiq 
Published: January 2020

AbstractThere are currently no standardised guidelines on the optimum dressing used for graft donor sites. The aim was to compare the outcomes of human amniotic membrane (HAM) vs routine dressings in split‐thickness skin graft (STSG) donor site healing. A systematic review and meta‐analysis was performed and a search of electronic information was conducted to identify all randomised controlled trials comparing the outcomes of HAM vs routine dressings in STSG donor sites. Wound healing and infection rate were primary outcome measures. Secondary outcome measures included severity of pain, discharge from donor site, the number of dressing changes, pruritus, and comfort. Fixed effect modelling was used for the analysis. Four studies enrolling 157 patients were identified. There was a significant difference between HAM and routine groups with wound healing time (P < .0001) and proportion of wounds healed by day 12 (P = .01). There was no significant difference between the two groups in infection rates (P = .27). For all secondary outcomes, HAM had improved results. HAM dressings are a superior option when compared with routine dressings used in current clinical practice for STSG donor sites as they improve wound healing and do not increase the infection rate.
Mutually exclusive antiproliferative effect of cell line‐specific HOX inhibition in epithelial ovarian cancer cell lines: SKOV‐3 vs RMUG‐S
Authors: Kim Miseon , Suh Dong Hoon , Choi Jin Young , Lee Seul , Bae Jae Ryul , Kim Kidong , No Jae Hong , Kim Yong Beom 
Published: January 2020

AbstractWe aimed to discover cell line‐specific overexpressed HOX genes responsible for chemoresistance and to identify the mechanisms behind HOX‐induced cell line‐specific chemoresistance in EOC. Ten HOX genes and eight EOC cell lines were tested for any cell line‐specific overexpression that presents a mutually exclusive pattern. Cell viability was evaluated after treatment with cisplatin and/or siRNA for cell line‐specific overexpressed HOX genes. Immunohistochemical (IHC) staining for HOXB9 was performed in 84 human EOC tissues. HOXA10 and HOXB9 were identified as cell line‐specific overexpressed HOX genes for SKOV‐3 and RMUG‐S, respectively. Inhibiting the expression of cell line‐specific HOX genes, but not of other HOX genes, significantly decreased cell viability. In SKOV‐3 cells, cell viability decreased to 46.5% after initial 10 µM cisplatin treatment; however, there was no further decrease upon additional treatment with HOXA10 siRNA. In contrast, cell viability did not significantly decrease upon cisplatin treatment in RMUG‐S cells, but decreased to 65.5% after additional treatment with HOXB9 siRNA. In both cell lines, inhibiting cell line‐specific HOX expression enhanced apoptosis but suppressed the expression of epithelial‐mesenchymal transition (EMT) markers such as vimentin, MMP9, and Oct4. IHC analysis showed that platinum‐resistant cancer tissues more frequently had high HOXB9 expression than platinum‐sensitive cancer tissues. HOXB9, which is overexpressed in RMUG‐S but not in SKOV‐3 cells, appeared to be associated with cell line‐specific platinum resistance in RMUG‐S. Inhibiting HOXB9 overexpression in RMUG‐S cells may effectively eliminate platinum‐resistant ovarian cancer cells by facilitating apoptosis and inhibiting EMT.
Combined tazemetostat and MAPKi enhances differentiation of papillary thyroid cancer cells harbouring BRAFV600E by synergistically decreasing global trimethylation of H3K27
Authors: Fu Hao , Cheng Lin , Sa Ri , Jin Yuchen , Chen Libo 
Published: January 2020

AbstractClinical efficacy of differentiation therapy with mitogen‐activated protein kinase inhibitors (MAPKi) for lethal radioiodine‐refractory papillary thyroid cancer (RR‐PTC) urgently needs to be improved and the aberrant trimethylation of histone H3 lysine 27 (H3K27) plays a vital role in BRAFV600E‐MAPK‐induced cancer dedifferentiation and drug resistance. Therefore, dual inhibition of MAPK and histone methyltransferase (EZH2) may produce more favourable treatment effects. In this study, BRAFV600E‐mutant (BCPAP and K1) and BRAF‐wild‐type (TPC‐1) PTC cells were treated with MAPKi (dabrafenib or selumetinib) or EZH2 inhibitor (tazemetostat), or in combination, and the expression of iodine‐metabolizing genes, radioiodine uptake, and toxicity were tested. We found that tazemetostat alone slightly increased iodine‐metabolizing gene expression and promoted radioiodine uptake and toxicity, irrespective of the BRAF status. However, MAPKi induced these effects preferentially in BRAFV600E mutant cells, which was robustly strengthened by tazemetostat incorporation. Mechanically, MAPKi‐induced decrease of trimethylation of H3K27 was evidently intensified by tazemetostat in BRAFV600E‐mutant cells. In conclusion, tazemetostat combined with MAPKi enhances differentiation of PTC cells harbouring BRAFV600E through synergistically decreasing global trimethylation of H3K27, representing a novel differentiation strategy.
Sensitive enzyme‐linked immunosorbent assay and gold nanoparticle immunochromatocgraphic strip for rapid detecting chloramphenicol in food
Authors: Wu Shih‐Wei , Wang Min‐Ying , Liu Biing‐Hui , Yu Feng‐Yih 
Published: January 2020

AbstractAntibody specific to chloramphenicol (CAP) was produced from rabbit that had been immunized with CAP‐keyhole limpet hemocyanin (KLH). Using the antibodies, we established a sensitive direct competitive enzyme‐linked immunosorbent assay (dcELISA) and a gold nanoparticle immunochromatographic strip (immunostrip) for detection CAP in food samples. In the dcELISA, CAP at levels of 0.15 ng/ml causes 50% inhibition (IC50) of the binding of CAP‐horseradish peroxidase to the antibodies. The overall analytical recoveries of CAP (0.25–100 ng/g) added to the honey or milk samples in the dcELISA were 81.9 and 73.7%, respectively. Onsite determination of CAP was accomplished by immunostrips with a detection limit of 0.5 ng/ml and completed within 10 min. Carefully studying 10 honey and 6 milk samples using the dcELISA and immunostrip indicated that all examined samples were negative for CAP. The presented dcELISA and immunostrip methods are sensitive enough for the rapid determination of CAP in the samples.
Comfort and Attitudes Towards Robots Among Young, Middle‐Aged, and Older Adults: A Cross‐Sectional Study
Authors: Backonja Uba , Hall Amanda K. , Painter Ian , Kneale Laura , Lazar Amanda , Cakmak Maya , Thompson Hilaire J. , Demiris George 
Published: January 2020

AbstractPurposeTo explore the social impact of, comfort with, and negative attitudes towards robots among young, middle‐aged, and older adults in the United States.DesignDescriptive, cross‐sectional. Conducted in 2014–2015 in an urban area of the western United States using a purposive sample of adults 18 years of age or older.MethodsRespondents completed a survey that included the Negative Attitudes Toward Robots Scale (NARS) and two questions taken or modified from the European Commission's Autonomous System 2015 Report. Analyses were conducted to compare perceptions and demographic factors by age groups (young adults:18–44, middle‐aged adults: 45–64, and older adults: >65 years old).FindingsSample included 499 individuals (n = 322 age 18–44 years, n = 50 age 45–64 years, and n = 102 age 65–98 years). There were no significant differences between age groups for 9 of the 11 items regarding social impact of robots and comfort with robots. There were no significant differences by age groups for 9 of the 14 items in the NARS. Among those items with statistically significant differences, the mean scores indicate similar sentiments for each group.ConclusionsOlder, middle‐aged, and younger adults had similar attitudes regarding the social impact of and comfort with robots; they also had similar negative attitudes towards robots. Findings dispel current perceptions that older adults are not as receptive to robots as other adults. This has implications for nurses who integrate supportive robots in their practice.Clinical RelevanceNurses working in clinical and community roles can use these findings when developing and implementing robotic solutions. Understanding attitudes towards robots can support how, where, and with whom robots can be used in nursing practice.
Exploring dementia care competence of nurses working in acute care settings
Authors: Yang Yueh‐Ying , Hsiao Chun‐Hui , Chang Ying‐Ju , Ma Shu‐Ching , Wang Jing‐Jy 
Published: January 2020

AbstractAims and ObjectivesTo examine the current status of dementia care competence of nurses working in acute care settings as well as the relationship between competence and demographic attributes.BackgroundMost people with dementia are older individuals when they are admitted to unfamiliar acute care settings for treatment, and they are prone to displaying BPSD. If nurses working in acute care settings are not sufficiently competent in dementia care, providing proper patient care is difficult.DesignThe study used a one‐sample descriptive‐correlation design.MethodsThis study enrolled nurses at two medical centres in southern Taiwan as the research participants and performed a stratified random sampling according to the units where they served. The Dementia Care Competence Scale was used for data collection. An independent samples t test, ANOVA and Pearson's product–moment correlation were performed for data analysis (See STROBE).ResultsA total of 308 valid questionnaires were collected. The results showed that dementia care competence of nurses working in acute care settings was moderate. In particular, they had insufficient knowledge of the special needs related to dementia and lacked the skills and patience necessary for identifying, preventing and managing BPSD. Additionally, although the nurses tended to have a positive attitude, they seldom communicated with people with dementia. This study also found that dementia care competence was better in nurses who were older, who had more seniority, who had taken care of people with dementia for a longer period of time and who had received training in dementia care.ConclusionsDementia care training topics for nurses working in acute care settings should include palliative care for dementia, skills for managing behavioural and psychological symptoms of dementia and communication techniques for improving person‐centred care. Nurses should also be encouraged to maintain a warm, friendly attitude when providing patient care.Relevance to Clinical PracticeContinuing education in managing behavioural and psychological symptoms of dementia is necessary for currently practicing acute care nursing staff and should be developed according to the staff's educational background and needs.
Stimulated by insight: Exploration of critical care nurses' experience of research participation in a recovery programme for intensive care survivors
Authors: Lehmkuhl Lene , Egerod Ingrid , Overgaard Dorthe , Bestle Morten H. , Jensen Janet F. 
Published: January 2020

AbstractAims and objectivesTo explore critical care nurses' experiences of research participation during a one‐year recovery programme for intensive care survivors.BackgroundNurse‐led postintensive care follow‐up consultations have emerged to help patients to recover and overcome problems related to critical illness and admission at the intensive care unit (ICU). Previous research exploring post‐ICU follow‐up programmes have shown inconclusive evidence of their effectiveness on patient‐reported outcome measurements, and provider evaluation is scarce. The context of this study is the Recovery and Aftercare in Postintensive care Therapy (RAPIT) trial.DesignA qualitative descriptive telephone interview study.MethodsData were collected after completion of the RAPIT trial. Participants (n = 14) were trained intensive care nurses, who delivered the post‐ICU recovery programme, representing nine out of ten sites from the RAPIT trial. Two focus group discussions were used to construct a semistructured interview guide. A thematic data analysis was performed using Braun and Clark's six‐step method. This study conforms to the COREQ Research Reporting Guidelines for qualitative studies.ResultsOur study indicated that nurses considered participation in research as a positive experience. The main finding “Stimulated by insight” described how nurses' engagement and professional growth was gained by reflection, patient feedback and research competencies acquired in the clinical setting. The research programmes stimulated to new knowledge, broaden their perspectives and enhanced critical reflection of ICU nursing practice.ConclusionsThe study indicates that nurses developed research competencies and enhanced their job satisfaction by using critical reflection and patient feedback. However, there is still a substantial need for support to strengthen nurses' competencies in collaboration with colleagues, managers and researchers.Relevance to Clinical PracticeThis study can contribute to the development of recommendations supporting nurses doing research and to optimise implementation of clinical research.
A concept analysis of psychological distress in parents related to diabetes management in children and adolescents
Authors: Arabiat Diana , AL Jabery Mohammad , Whitehead Lisa 
Published: January 2020

AbstractPurposeTo report a concept analysis of parents' psychological distress in the context of diabetes management among children and adolescents. A clear understanding of the possible impact of diabetes management on parents will help to inform how nurses can work with parents to support glycaemic control in children and adolescents.Design and MethodConcept analysis using Walker and Avant's eight‐stage approach was used as a guiding framework. PubMed, OVID (CINAHL, Medline, PsychInfo), the Cochrane library and the Joanna Briggs library were searched for the past 50 years.ResultsThirty‐three studies provided data for the concept analysis. Attributes included difficulty coping, changes in emotional status and manifestations of mental health problems.Practice ImplicationBased on the literature synthesis, we suggest all facets of distress related to diabetes can in principle be inferred through the proposed relationship between distress and other interactions of individual coping, caring burden and family relational functioning. The proposed conceptual model linking antecedents' factors and individual characteristics of parents to the concepts of psychological distress may assist researchers to design interventions for supporting diabetes management in children and adolescents.
Self‐perception of symptoms, medical help seeking, and self‐help strategies of women with interstitial cystitis/painful bladder syndrome
Authors: Chen Hui‐Chun , Liu Chieh‐Yu , Liao Chun‐Hou , Tsao Lee‐Ing 
Published: January 2020

AbstractBackgroundThis study aims to investigate the self‐perception of symptoms, medical help seeking, and self‐help strategies of women with interstitial cystitis (IC).MethodsA mixed method of qualitative and quantitative approaches was employed. The qualitative approach used in‐depth interviews about the subjective experience of symptoms, medical help seeking, and self‐help strategies for their IC. The quantitative inquiry was conducted by a yes or no response to the question “Did self‐perceived severe symptoms of IC interfere with your daily life?” A loglinear model was applied to investigate the associations between possible factors.ResultsThis study recruited 68 women aged 20 to 69 years, of whom 22 were interviewed for qualitative data. About 72.1% of the women responded that self‐perceived severe IC symptoms interfered with their daily life. A significant negative association between employment and self‐perceived severe IC symptoms (P < .05) was observed. Qualitative results revealed three important themes: (1) bothersome symptoms—all‐day bladder pain and lower urinary tract symptoms and deteriorated quality of life, (2) medical help seeking—exhaustion and frustration, (3) self‐help strategies—coexisting with IC or feeling helpless.ConclusionsIC women feel exhausted and frustrated by seeking medical attention for this incurable disease for a long time. IC women have troubled and uneasy daily lives. Being employed or engaging in activities can divert attention to alleviate symptoms. IC patient support groups allow patients to share their self‐help experiences with interdisciplinary medical teams to provide physical and psychological treatment.
Magnesium isotopes of the bulk solar wind from Genesis diamond‐like carbon films
Authors: Jurewicz A. J. G. , Rieck K. D. , Hervig R. , Burnett D. S. , Wadhwa M. , Olinger C. T. , Wiens R. C. , Laming J. M. , Guan Y. , Huss G. R. , Reisenfeld D. B. , Williams P. 
Published: January 2020

AbstractNASA's Genesis Mission returned solar wind (SW) to the Earth for analysis to derive the composition of the solar photosphere from solar material. SW analyses control the precision of the derived solar compositions, but their ultimate accuracy is limited by the theoretical or empirical models of fractionation due to SW formation. Mg isotopes are “ground truth” for these models since, except for CAIs, planetary materials have a uniform Mg isotopic composition (within ≤1‰) so any significant isotopic fractionation of SW Mg is primarily that of SW formation and subsequent acceleration through the corona. This study analyzed Mg isotopes in a bulk SW diamond‐like carbon (DLC) film on silicon collector returned by the Genesis Mission. A novel data reduction technique was required to account for variable ion yield and instrumental mass fractionation (IMF) in the DLC. The resulting SW Mg fractionation relative to the DSM‐3 laboratory standard was (−14.4‰, −30.2‰) ± (4.1‰, 5.5‰), where the uncertainty is 2ơ SE of the data combined with a 2.5‰ (total) error in the IMF determination. Two of the SW fractionation models considered generally agreed with our data. Their possible ramifications are discussed for O isotopes based on the CAI nebular composition of McKeegan et al. (2011).