bluuebunny/crossref_metadata_2025_split huggingface dataset:
A modified technique for paravaginal repair for the treatment of anterior vaginal prolapse and cystocele: a retrospective study
Authors: Huang Shuai , Lv Qiubo , Li Ye , Li Min , Zhang Sichen 
Published: June 2020

Abstract
Background

We aimed to determine the efficacy and safety of a modified protocol for paravaginal repair for treating symptomatic paravaginal defect cystocele.
Methods

This study was an observational case series of 98 consecutive female patients, referred to our hospital between June 2014 and May 2018, with symptomatic grade II to IV paravaginal defects and cystocele. Our modified technique for paravaginal repair is based on the conventional protocol but incorporates reverse bridge repair and the cross-stitching of bilateral sutures. The curative effect of this new technique was evaluated subjectively and objectively during postoperative follow-up.
Results

All operations were successful. Patients were followed up for 12 to 48 months, until June 2019; the mean follow-up period was 32.4 months. Three months after surgery (98 cases), the subjective cure rate was 100%; in each case, the top of the vagina lay above the level of the sciatic spine. The objective cure rate was 100%. The objective cure rate was 94.9% (93 cases) twelve months after surgery (98 cases), 91.0% (61 cases) twenty-four months after surgery (71 cases), and 76.2% (16 cases) forty-eight months after surgery (21 cases). Four cases required a second round of surgery; three of these cases were treated with sacrocolpopexy, and one case was treated with sacrospinous ligament fixation.
Conclusion

Our modified technique for paravaginal repair was safe and effective for the treatment of anterior vaginal prolapse and cystocele, as confirmed by the results observed over a mean follow-up period of 32.4 months.
Application of Various Surgical Techniques in Orthotopic Liver Transplantation: a 10-year Experience at a Single Center
Authors: Chen Zhitao , Han Ming , Ju Weiqiang , Chen Maogen , He Xiaoshun 
Published: June 2020

Abstract
BackgroundLiver transplantation (LT) is considered the only curative treatment for end-stage liver disease (ESLD), and the surgical techniques of LT have continually evolved and have been modified. In this study, we prospectively analyzed a single-center case series in our center and compared the advantages and disadvantages of each method.MethodsIn total, 1,029 patients with OLT at our department were enrolled in this study. The recipient perioperative data were assessed and analyzed. Three types of LT techniques were utilized: modified classic, modified piggy-back (MPB) and classic piggy-back (PB) orthotopic LT, corresponding to groups A, B and C.ResultsCirrhosis was the most prevalent condition in group B, while tumors tended to be more common in group A. Patients in group C were in poorer general condition with higher creatinine, total bilirubin and PT-INR (P = 0.029, 0.011 and 0.026, respectively). Two hundred ninety-five patients had previous abdominal surgery, and the proportion was higher in group B (P = 0.017). The cold ischemia time in group B was longer than those in the other two groups (P<0.001). The mortality rate was 7.9% within 30 days and 11.7% within 90 days. Most of the deaths were not technique-related.ConclusionThe advantages and disadvantages are different for these three surgical techniques. A reasonable operation technique should be adopted considering the patient's unique condition to ensure the stability of hemodynamics.
Circulating Tumor Cells to Predict Microvascular Invasion and Dynamical Detection Indicate Prognosis of Hepatocellular Carcinoma
Authors: Zhou Jiangmin , Zhang Zhiwei , Zhou Honghao , leng Chao , Hou Bingwu , Zhou Chenyang , Hu Xinsheng , Wang Jinlin , Chen Xiaoping 
Published: June 2020

Abstract
Background: The number of preoperative CTC was correlated with the presence of MVI. The change of postoperative CTC count can predict the prognosis of patients. But the change trend of postoperative CTC is controversial.Methods: A total of 137 patients were recruited for the study. Preoperative blood samples of all patients were collected to detect CTC. The time points for blood collection were before the operation, during operation, and at one week, one month, two months, three months, six months, and one year after surgery. The power of predicting the presence of MVI was analyzed by the receiver operating characteristic curve (ROC). According to the survival status, 137 patients were divided into three groups: no recurrence, early recurrence, and non-early recurrence. Results: A threshold CTC value of 5 showed the most significant power to predict the existence of MVI. In a multivariate analysis, the parameters of preoperative CTCs, AFP, and tumor diameter were independent predictors of MVI (P < 0.05). A CTC value greater than or equal to 5 has better predictive value than AFP > 400μg/L or tumor diameter. The number of intraoperative CTCs in the three groups did not increase compared to before surgery (P > 0.05). The number of CTC in the non-recurrent group and the non-early recurrent group decreased significantly one week after surgery compared with intraoperative values (P < 0.001), although there was no statistical significance in the group with early recurrence (P = 0.95). The mean CTC in the early recurrence group was higher than that in the other two groups (P <0.001). Conclusion: The preoperative CTC counts in peripheral blood of patients with HCC is closely correlated to MVI. Intraoperative manipulation for the lesion by the surgeon does not increase the number of CTC in peripheral blood. Surgical removal of the tumor decreases the number of CTC. The persistence of CTC at a high level (≥ 5) after surgery suggests a risk of early recurrence.
The landscape of tumor mutational burden and its clinical significance in patients with lung cancer: a Multi-omics study with a meta-analysis
Authors: Wang Ying , Zhu Linhai , Xia Pinghui , Wang Zhitian , Yu Li , Lv Wang , Hu Jian 
Published: June 2020

Abstract
Background: Current research on tumor mutational burden (TMB) has focused on tumor immunotherapy responsiveness, but the role of TMB in non-immunotherapy patients is unclear. The purpose of this study is to explore the effect of TMB on lung cancer patients in order to clarify and expand the clinical significance of TMB in lung cancer.Methods: We download mutation data of lung cancer cases from The Cancer Genome Atlas (TCGA) database to analyze TMB and its composition, and study the relationship between TMB and clinicopathological characteristics of lung cancer patients. We then systematically retrieved and analyzed studies on the relationship between TMB and survival outcomes. The hazard ratio (HR) and its 95% confidence interval (CI) were used as an effective size to assess the survival outcomes. The subgroup analyses based on the pathological type, treatment method, TMB detection method and detection materials were also performed to explore the factors that might affect the interpretation of TMB results.Results: TMB in lung squamous cell carcinoma is lower than those in lung adenocarcinoma. In lung adenocarcinoma, patients with EGFR mutation have lower TMB than patients with EGFR wild-type. The summary analysis found that TMB is a better prognostic factor in small cell lung cancer, and more evident in small cell lung cancer receiving immunotherapy. TMB is a neutral or poor prognostic indicator in non-small cell lung cancer, but a better prognostic factor in non-small cell lung cancer receiving immunotherapy. In patients with lung adenocarcinoma, including those with EGFR mutation and receiving EGFR-targeted therapies, high TMB means worse survival. TMB detected by blood specimens is inconsistent and unstable compared to TMB detected by tissue. The clinical significance of TMB from blood specimens needs further study on extensive sample data.Conclusions: The pooled results indicated that TMB is a good prognostic factor in lung cancer patients receiving immunotherapy. But high TMB is connected with worse survival in non-small cell lung cancer without receiving immunotherapy, especially in lung adenocarcinoma. For lung adenocarcinoma patients with both EGFR mutation and high TMB, how to make a choice between EGFR-targeted therapy and immunotherapy is still a problem that requires further research.
Urinary Tract Infection Caused By Enterococcus Spp: Risk Factors And Mortality. An Observational Study.
Authors: Alvarez-Artero Elisa , Nuñez Amaia Campo , Garcia Inmaculada Garcia , Bravo Moises Garcia , Cores Olia , Perez Inmaculada Galindo , Pendones Jose , Bernus Amparo Lopez , Belhassen-Garcia Moncef , Lleidas Javier Pardo 
Published: June 2020

Abstract
Background
Urinary tract infections (UTIs) are frequently caused by Enterococcus spp. We aim to define the risk factors involved in UTIs caused by Enterococcus. Determine the overall mortality and predictive risk factors.
Methods
A retrospective in-patients study was conducted with bacteriemic UTIs caused by Enterococcus spp. We compared bacteriemic UTIs caused by Enterococcus spp. vs. a random sample of 100 in-patients with bacteriemic UTIs caused by others enterobacteria.
Results
We found 106 in-patients with UTIs caused by Enterococcus spp., 51 of whom had concomitant positive blood cultures. Distribution by species was: 83% E. faecalis and 17% E. faecium, with a Charlson comorbidity index of 5.9 ± 2.9. When we compared bacteriemic UTIs caused by Enterococcus spp. vs. bacteriemic UTIs caused by others enterobacteria we found the following independent predictors of bacteriemic UTI by Enterococcus: male sex with an OR of 6.1 (95%CI 2.3–16.1), uropathy with an OR of 4.1 (1.6–10.1), nosocomial infection with an OR of 3.8 (1.4–10.3), urinary cancer with an OR of 6.4 (1.3–30.3) and previous antimicrobial treatment with an OR of 18 (5.2–62.1). Overall, in-patient mortality was 16.5%, which was associated with a higher Sequential Organ Failure Assessment (SOFA) score (> 4), severe comorbidity such as immunosuppression, malignant hemopathy and nephrostomy, or Enterococcus faecium species and its pattern or resistance to ampicillin or vancomycin (p < 0.05). Appropriate empiric antibiotic therapy was not associated with a better prognosis (p > 0.05).
Conclusions
Enterococcus spp. is a frequent cause of complicated UTI by a profile of risk factors. High mortality secondary to a severe clinical setting and high comorbidity may be sufficient reasons for implementing empiric treatment of patients at risk, although we did not show a higher survival rate in patients with this treatment strategy.
Genome-wide identification and expression analysis of beta-galactosidase family members in sweetpotato [ Ipomoea batatas (L.) Lam.]
Authors: li zongyun , hou fuyun , qin zhen , Du Taifeng , Xu Tao , Li Aixian , Dong Shunxu , Ma Daifu , Wang Qingmei , Zhang Liming 
Published: June 2020

Abstract
Background: Sweetpotato (Ipomoea batatas (L.) Lam.) serves as an important food source for human beings. β-galactosidase (β-gal) is a glycosyl hydrolase involved in cell wall modification, which plays essential roles in plant development and environmental stress adaptation. However, the function of β-gals genes in sweetpotato has yet to be reported.Results: In this study, 17 β-galactosidase genes (Ibbgal) were identified in sweetpotato, which were classified into seven subfamilies using interspecific phylogenetic and comparative analyses. The promoter regions of Ibbgals harbored several stress, hormone and light responsive cis-acting elements. The Ibbgal genes were specifically expressed in different tissues and varieties, and differentially expressed under various hormonal treatments, and abiotic and biotic stresses.Conclusions: These findings suggest that Ibbgals may involve in plant development and stress responses through regulating the metabolism of cell wall polysaccharides.
Long non-coding NEAT1 weakens the protective role of sevoflurane on myocardial ischemia/reperfusion injury by mediating the microRNA-140/RhoA axis
Authors: Rui Pengfei , Wang Junhua , Xu Jing 
Published: June 2020

Abstract
Background: The inhaled Sevoflurane (Sev) has been implicated to protect myocardial tissues against ischemia/reperfusion (I/R)-evoked injury. Nevertheless, the detailed mechanisms of Sev‐mediated cardioprotection remain basically unknown. This study intends to examine the roles of nuclear enriched abundant transcript 1 (NEAT1), a long non-coding RNA (lncRNA) during the cardioprotection exerted by Sev.Methods: We initially performed I/R surgery in mice, and Sev treatment was given during the perfusion, followed by the determination of cardiac function, myocardial infarction area and myocardial apoptosis for the validation of the model. Then, we conducted lncRNA microarray analysis on Sev-treated hearts to find out lncRNAs with significant differences in expression. The changes of myocardial I/R injury were evaluated by echocardiography, TTC staining and TUNEL assay after establishing mice with NEAT1 upregulation. Afterwards, the targeting miRNA of NEAT1 and the targeting mRNA of the miRNA were screened out through StarBase and microarray analyses to carry out rescue experiments.Results: NEAT1 was downregulated in Sev-treated cardiomyocytes. Overexpression of NEAT1 weakened cardiac function, increased infarct size and increased apoptosis in the presence of Sev. NEAT1 bound to microRNA (miR)-140, which was significantly upregulated in Sev-treated cardiomyocytes. Inhibition of miR-140 expression weakened the cardiac function of Sev-treated mice, increased infarct size and cardiomyocyte apoptosis. miR-140 mediated the RhoA pathway, and the RhoA activity inhibition attenuated miR-140 effects on Sev protection.Conclusion: Sev mitigates myocardial I/R injury by restoring NEAT1 expression and regulating the miR-140/RhoA axis.
A risk score-matched model analysis of 4,452 patients with small cell lung cancer: who will benefit from operative treatment
Authors: Tan Shuzhen , Kong Ying , Leng Shuilong , Zhu Xiao 
Published: June 2020

Abstract
Purpose: Small-cell lung cancer (SCLC) is difficult to cure. In this study, the SEER database was used to construct a model and explore the potential prognostic factors of SCLC patients. Methods: The data were sorted out and randomly divided into training cohort and verification cohort. Univariate and multivariate Cox regression were used in the training cohort to analyze the independent prognostic factors, then they be incorporated into the Nomogram model. Using the C-index, calibration algorithm and ROC in conjunction with the risk scores, the model was verified with the verification cohort. Finally, the overall survivals of those factors were evaluated in the total cases.Results: In the training cohort, we found that age, race, sex, total stage and extension were independent factors which were included in the Nomogram model. C-index(s) that obtained from the training and verification cohorts showed that the model has predictive power. Moreover, the calibration curves and AUC results proved that the model is of great consistency not only in the training cohort but also in the verification cohort. Finally, significant differences in survival were observed among the above-mentioned factors and the overall survivals decreased over time.Conclusions: Age, race, sex, total stage and extension degree are independent risk factors for overall survival of patients. The Nomogram model can better predict the 1-year, 3-year and 5-year survival probabilities, providing accurate reference for clinical individualized treatment.
Distal sociological determinants and their association with the spatial distribution of new diagnoses of HIV infection in small areas in Catalonia (Spain)
Authors: Agusti Cristina , Font-Casaseca Núria , Belvis Francesc , Julià Mireia , Vives Núria , Montoliu Alexandra , Casabona Jordi , Benach Joan 
Published: June 2020

Abstract
Background
Spatial visualization of HIV surveillance data could improve the planning of programs to address the HIV epidemic.
Methods
Surveillance data from the national registry were presented in the form of descriptive and ring maps and used to study the spatial distribution of new HIV diagnoses in Catalonia (2012–2016) and associated risk factors at the small area level (ABS, acronym for “basic health area in Catalan). Incident cases were modeled using the following as predictors: type of municipality, prevalence of young men and migrant groups, MSM activity indicators, and other variables at the aggregated level.
Results
New HIV diagnoses are heterogeneously distributed across Catalonia. The predictors that proved to be significantly associated with a higher rate of new HIV diagnoses were ABS located in the city of Barcelona (IRR, 2.520; P < 0.001), a higher proportion of men aged 15–44 years (IRR, 1.193; P = 0.003), a higher proportion of MSM (IRR, 1.230; P = 0.030), a higher proportion of men from Western Europe (IRR, 1.281; P = 0.003), a higher proportion of men from Latin America (IRR, 1.260; P = 0.003), and a higher number of gay locations (IRR, 2.665; P < 0.001). No association was observed between the HIV diagnosis rate and economic deprivation.
Conclusions
Ring maps revealed substantial spatial associations for the rate of new HIV diagnoses. New HIV diagnoses are concentrated in ABS located in urban areas. Our results show that the socioeconomic deprivation index on which the Catalan government bases its budget allocation policies among the ABS may not be appropriate for all diseases.
RT-PCR Status and Antibody Response in Patients with Coronavirus Disease 2019
Authors: Chen Lan , Zhang Xiao-Bin , Zhang Zhen-Yu , Zhang Su-Zhen , Han Qiu-Ying , Feng Zhi-Peng , Xiao Xiong , Chen Hui-Min , Chen Xian-Li , Lan Yu-Pei , Yin Zhen-Yu 
Published: June 2020

Abstract
Background: Novel coronavirus (COVID-19) is a new viral species that causes pneumonia. Currently, RT-PCR and IgM/IgG antibody assays have been recommended for the diagnosis of COVID-19 infection. However, the correlation between RT-PCR status and antibody (IgG, IgM) response remains unknown. Methods: Consecutive COVID-19 patients admitted to our department between February 10, 2020 and March 10, 2020, were diagnosed by guidelines issued by the World Health Organization (WHO) and included in this study. RT-PCR and antibody (IgM/IgG) assays for COVID-19 infection were performed for all patients according to the manufactures’ protocols. Other data, such as demographic, clinical, laboratory, as well as treatment and outcome, were collected using data collection tables from electronic medical records.Results: During the study period, a total of 103 patients were diagnosed as having a moderate type of COVID-19 at our department, including 55 males and 48 females, with an average age of 57.53 ± 1.65 years old (range 23 to 90 years old). The peak level of SARS-CoV-2 IgM antibody (243.10 ± 89.84 AU/ml) was reported 4 days after the negative RT-PCR (-) (all P < 0.05). Subsequently, the IgM decreased to 42.69 ± 22.39 AU/ml 21 days after RT-PCR (-). However, the IgG was maintained at a high level 4 days before RT-PCR (-) and later. The lymphocyte count was at the lowest level on day7 before the RT-PCR(-) result (P<0.05), and then elevated after RT-PCR conversion (viral clearance).Conclusions: SARS-CoV-2 IgM/IgG levels did not correlate with RT-PCR status in our study sample. We found that SARS-CoV-2 IgM/IgG could be a potential biomarker to monitor clinical course, determine discharge, and assess recovery of those infected patients with the novel coronavirus. Trial registration: A prospective, open label, randomized, control trial for chloroquine or hydroxychloroquine in patients with mild and common novel coronavirus pulmonary (COVIP-19). ChiCTR2000030054. Registered 18 Feb,2020. http://www.chictr.org.cn/edit.aspx?pid=49869&htm=4
Skin cancer risk factors among black South Africans — the Johannesburg Cancer Study, 1995–2016
Authors: Ndlovu Babongile Confidence , Sengayi-Muchengeti Mazvita , Wright Caradee Y , Chen Wenlong C , Kuonza Lazarus , Singh Elvira 
Published: June 2020

Abstract
Background
The Black population is known to have lower risk for skin cancers due to melanin content of the skin. Regardless, skin cancers still occur in Black populations. The aim of this study was to identify risk factors associated with skin cancer among Blacks presenting at selected tertiary hospitals in Johannesburg, South Africa.
Methods
A case-control study was conducted; cases were patients with keratinocyte cancers (KCs) and/or melanoma skin cancer (MSC) and controls were cardiovascular patients. Socio-demographic exposures (sex and residency), environmental exposures (heating and cooking fuels), smoking, and HIV status were assessed. The proportions of cases by skin cancer major subtype, demographics, histological spectrum and anatomical site of distribution were determined. A stepwise (backward elimination) logistic regression was done to identify risk factors associated with KC and MSC.
Results
More KCs (n = 160) were found compared to MSCs (n = 101). The majority of both KCs and MSCs were reported in ages 51-60-years (27%). The median age at KC and MSC diagnosis was similar in both sexes; 50-years (IQR:38–57) and 56-years (IQR:48–68), respectively. The KC histological spectrum showed that there were more squamous cell carcinomas (SCCs) (78/160 in females, and 72/160 in males) than basal cell carcinoma (BCC). The SCC lesions were mostly found on the skin of the head and neck in males (51%, 38/72) and on the trunk in females (46%, 36/78). MSC was shown to affect the skin of the lower limbs in both males (68%, 27/40) and females (59%, 36/61). Using females as the reference group, when age, current place of residency, type of cooking fuel used currently, smoking, and HIV status were adjusted for, males had an odds ratio (OR) of 2.04 for developing KC (CI:1.08–3.84, p = 0.028). Similarly, when age, current place of residency, place of cooking (indoors or outdoors) were adjusted for, males had an OR of 2.26 for developing MSC (CI:1.19–4.29, p = 0.012).
Conclusions
Differences in anatomical distribution of KCs by sex suggest different risk factors between sexes. Rural dwelling was a newly found association to skin cancer and warrants further investigation. This study highlights the importance of skin cancer awareness campaigns and interventions especially in rural areas.
Effects of interfering plasmid on the pathogenicity of gastric cancer by cell proliferation and apoptosis testing
Authors: Geng xingliang , Li Weidong , liao guoyang 
Published: June 2020

Abstract
Background

To study the effects of recombinant interfering plasmids (pCDNA3.1-miR340) on the pathogenicity of gastric cancer by assessing cell proliferation and apoptosis.
Methods

Microarrays were used to analyse the function of pCDNA3.1-miR340. Reverse transcription polymerase chain reaction (RT-PCR) was used to evaluate miRNA-340 expression in different patients, while the pCDNA3.1-miR340 plasmid was constructed for use in wound-healing and migration assays. Expression of the miRNA-340 target, RhoA, was assessed by Western blotting.
Results

miRNA-340 expression was significantly higher in patients with gastric cancer. Treatment with pCDNA3.1-miR340 significantly slowed tumour growth compared to treatment with empty plasmid. Additionally, when miRNA-340 expression was reduced, apoptosis decreased significantly, while RhoA protein expression increased 1.90-2.02-fold in SGC-7901 cells.
Conclusions

pCDNA3.1-miR340 had a positive therapeutic effect on the pathogenicity of gastric cancer.