bluuebunny/crossref_metadata_2025_split huggingface dataset:
Group A streptococcal bacteremias in Southwest Finland 2007–2018: epidemiology and role of infectious diseases consultation in antibiotic treatment selection
Authors: Vilhonen Johanna , Vuopio Jaana , Vahlberg Tero , Gröndahl-Yli-Hannuksela Kirsi , Rantakokko-Jalava Kaisu , Oksi Jarmo 
Published: July 2020

AbstractThe incidence of invasive group A streptococcal (GAS) infections has shown a fluctuating but increasing trend in Finland. The impact of infectious diseases specialist consultation (IDSC) on the antimicrobial therapy of GAS bacteremia has not been studied earlier. A retrospective study on adult GAS bacteremia in The Hospital District of Southwest Finland (HDSWF) was conducted from 2007 to 2018. Data on incidence of bacteremic GAS cases were gathered from the National Infectious Disease Register. Clinical data were obtained by reviewing the electronic patient records. The overall incidence of GAS bacteremia in HDSWF was 3.52/100,000, but year-to-year variation was observed with the highest incidence of 7.93/100,000 in 2018. A total of 212 adult GAS bacteremia cases were included. A record of IDSC was found (+) in 117 (55.2%) cases, not found (−) in 71 (33.5%) cases and data were not available in 24 (11.3%) cases. Among IDSC+ cases, 57.3% were on penicillin G treatment whereas in the group IDSC− only 22.5%, respectively (OR = 4.61, 95% CI 2.37–8.97; p < 0.001). The use of clindamycin as adjunctive antibiotic was more common among IDSC+ (54.7%) than IDSC− (21.7%) (OR = 4.51, 95% CI 2.29–8.87; p < 0.001). There was an increasing trend in incidence of GAS bacteremia during the study period. Narrow-spectrum beta-lactam antibiotics were chosen, and adjunctive clindamycin was more commonly used, if IDSC took place. This highlights the importance of availability of IDSC but calls for improved practice among infectious diseases specialists by avoiding combination therapy with clindamycin in non-severe invasive GAS infections.
The effect of berberine chloride and/or its combination with vancomycin on the growth, biofilm formation, and motility of Clostridioides difficile
Authors: Wultańska Dorota , Piotrowski Michał , Pituch Hanna 
Published: July 2020

AbstractThis study aims to investigate the antimicrobial and antibiofilm activity of berberine chloride (BBR) and vancomycin (VAN) as well as synergistic combinations of BBR with VAN againstClostridioides difficilestrains. The effect of different concentrations of BBR on strain motility was also assessed. TwelveC. difficilestrains (two referenceC. difficile630, ATCC 9689, and one control M120, and 9 clinicalC. difficilestrains belonging to the PCR-ribotype (RT027)) were collected and investigated for their susceptibility to BBR and VAN in planktonic and biofilm forms. Both the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of BBR for theC. difficilestrains were found to vary over a broad range (256–1.024 mg/L and 256–16.384 mg/L, respectively). The MIC and MBC of VAN also varied greatly, ranging from 0.25 to 4.0 mg/L for MIC and 0.25 to 64.0 mg/L for MBC. The synergistic effect of the sub-MIC (1/2 MIC) BBR with VAN reduced of MICs of VAN against the planktonic forms of tenC. difficilestrains. The sub-MIC of BBR enhanced the biofilm formation of one strain and was found to be statistically significant. In addition, the sub-MIC of BBR with VAN surprisingly enhanced the biofilm formation of oneC. difficilestrain. The effect of inhibition of motility in the presence of BBR was statistically significant for 3 clinical strains (p < 0.05). Altogether, BBR exhibited strong antimicrobial activity againstC. difficile, and the analysis of the combination of BBR with VAN showed a synergistic effect.
Detection of anti-Pneumocystis jirovecii antibodies in human serum using a recombinant synthetic multi-epitope kexin-based antigen
Authors: Tomás Ana Luísa , Cardoso Fernando , de Sousa Bruno , Matos Olga 
Published: July 2020

AbstractInterest in the detection of specific anti-Pneumocystis jirovecii antibodies has emerged as less-invasive alternative diagnostic approaches. Here is presented the performance of an ELISA based on a recombinant synthetic multi-epitope kexin 1 (Kex1) antigen of P. jirovecii, previously developed. Results showed that IgM anti-Kex1 levels were found significantly increased in patients with Pneumocystis pneumonia (PcP) compared with non-PcP cases (p < 0.001), allowing a diagnostic performance of PcP with a 70.8% sensitivity and a 75.0% specificity. These results suggest that this Kex1-based ELISA is a promising tool toward the serodiagnosis of PcP when the standard methods are difficult to perform.
Yeast as a promising heterologous host for steroid bioproduction
Authors: Xu Shanhui , Li Yanran 
Published: July 2020

Abstract
With the rapid development of synthetic biology and metabolic engineering technologies, yeast has been generally considered as promising hosts for the bioproduction of secondary metabolites. Sterols are essential components of cell membrane, and are the precursors for the biosynthesis of steroid hormones, signaling molecules, and defense molecules in the higher eukaryotes, which are of pharmaceutical and agricultural significance. In this mini-review, we summarize the recent engineering efforts of using yeast to synthesize various steroids, and discuss the structural diversity that the current steroid-producing yeast can achieve, the challenge and the potential of using yeast as the bioproduction platform of various steroids from higher eukaryotes.
Geoengineering super low carbon cows: food and the corporate carbon economy in a low carbon world
Authors: Ormond Jim 
Published: July 2020

AbstractThe tangible progress to address climatic change remains painfully slow. As a result, practices to deliberately manipulate the Earth’s carbon and energy cycles to counteract climate change have gained traction and they are increasingly incorporated into mainstream debate. This paper examines one of the less documented examples of climate geoengineering, namely the creation of ‘super low carbon cows’. Driven by the public’s desire for a low carbon pint of milk or beef burger, I show how a combination of bioengineering, technological fixes and management practices have resulted in, and are informing, everyday changes to the way in which animals are bred, cared for and eaten—and in turn, how it affects the food that we consume. Thus, the role of the cow within the Anthropocene now extends from meat machine and sentient being to climate change saviour. I seek to show that super low carbon cows represent part of a wider climate ‘responsibilisation’ in which business interests and corporate storytelling are governing and enacting everyday mundane practices of climate engineering as part of the corporate carbon economy. Yet, as with other climate ‘fixes’, this paper shows that the super low carbon cow provides, at best, an imperfect correction. Critical gaps in the evidence of the efficiency of the solutions being advanced remain whilst manipulating an animal to be more climate friendly evokes unease when considering the wider sustainability and ethical impacts. Perhaps most critically, reliance on climate engineering to provide cheap and easy ways to control our climate fails to question, far less address, the ever-increasing demand, production and wastage of food. It also potentially undermines the already weak political will for other essential and more radical responses to climate change. In doing so, I contrast the extensive efforts to change the everyday behaviours of a cow with the limited attempts to meaningfully challenge the everyday practices, consumption lifestyles and dietary choices of the general public.
Hydroxyethylcellulose as a methotrexate carrier in anticancer therapy
Authors: Ciekot Jarosław , Psurski Mateusz , Jurec Katarzyna , Boratyński Janusz 
Published: July 2020

AbstractClinical and experimental cancer therapy is multifaceted; one such facet is the use of drug carriers. Drug carriers are various nano- and macromolecules, e.g., oligosaccharides, proteins, and liposomes. The present study aimed to verify the suitability of cellulose as a carrier for methotrexate (MTX). Hydroxyethylcellulose, with a molecular weight of 90 kDa and soluble in water, was used. Methotrexate was linked to cellulose by methyl ester bonds. A conjugate containing on average 9.5 molecules of MTX per molecule of cellulose was developed. Gel filtration HPLC analysis showed that the conjugate contained approximately 2% free drug. Dynamic light scattering analysis showed an increase in the polydispersity of the conjugate. The degradation of the conjugate in phosphate buffer and plasma followed first-order kinetics. The conjugate showed the lowest stability (half-life 154 h) in plasma. The conjugate showed 10-fold lower cytotoxicity to the 4 T1 mammary tumour cell line than the free drug. In the in vivo experiment to treat orthotopically implanted mammary tumours, the conjugate and the free drug, both applied intravenously, showed maximum inhibition of tumour growth of 48.4% and 11.2%, respectively. In conclusion, cellulose, which is a non-biodegradable chain glucose polymer, can be successfully used as a drug carrier, which opens up new research perspectives.
Effects of preservation on protein extraction in four seaweed species
Authors: Wijers Tom , Hylkema Alwin , Visser Tsjippie , Timmermans Klaas 
Published: July 2020

AbstractUsing either freshly pulped or preserved seaweed biomass for the extraction of protein can have a great effect on the amount of protein that can be extracted. In this study, the effect of four preservation techniques (frozen, freeze-dried, and air-dried at 40 and 70 °C) on the protein extractability, measured as Kjeldahl nitrogen, of four seaweed species, Chondrus crispus (Rhodophyceae), Ascophyllum nodosum, Saccharina latissima (both Phaeophyceae) and Ulva lactuca (Chlorophyceae), was tested and compared with extracting freshly pulped biomass. The effect of preservation is species dependent: in all four seaweed species, a different treatment resulted in the highest protein extractability. The pellet (i.e., the non-dissolved biomass after extraction) was also analyzed as in most cases the largest part of the initial protein ended up in the pellet and not in the supernatant. Of the four species tested, freeze-dried A. nodosum yielded the highest overall protein extractability of 59.6% with a significantly increased protein content compared with the sample before extraction. For C. crispus extracting biomass air-dried at 40 °C gave the best results with a protein extractability of 50.4%. Preservation had little effect on the protein extraction for S. latissima; only air-drying at 70 °C decreased the yield significantly. Over 70% of the initial protein ended up in the pellet for all U. lactuca extractions while increasing the protein content significantly. Extracting freshly pulped U. lactuca resulted in a 78% increase in protein content in the pellet while still containing 84.5% of the total initial total protein. These results show the importance of the right choice when selecting a preservation method and seaweed species for protein extraction. Besides the extracted protein fraction, the remaining pellet also has the potential as a source with an increased protein content.
Comparison of covariate selection methods with correlated covariates: prior information versus data information, or a mixture of both?
Authors: Chasseloup Estelle , Yngman Gunnar , Karlsson Mats O. 
Published: July 2020

AbstractThe inclusion of covariates in population models during drug development is a key step to understanding drug variability and support dosage regimen proposal, but high correlation among covariates often complicates the identification of the true covariate. We compared three covariate selection methods balancing data information and prior knowledge: (1) full fixed effect modelling (FFEM), with covariate selection prior to data analysis, (2) simplified stepwise covariate modelling (sSCM), data driven selection only, and (3) Prior-Adjusted Covariate Selection (PACS) mixing both. PACS penalizes the a priori less likely covariate model by adding to its objective function value (OFV) a prior probability-derived constant: $$2*{\kern 1pt} \,{\ln}\left( {{\Pr}\left( X \right)/\left( {1 - {\Pr}\left( X \right)} \right)} \right)$$

2

∗

ln

Pr

X

/

1
-
Pr

X

, Pr(X) being the probability of the more likely covariate. Simulations were performed to compare their external performance (average OFV in a validation dataset of 10,000 subjects) in selecting the true covariate between two highly correlated covariates: 0.5, 0.7, or 0.9, after a training step on datasets of 12, 25 or 100 subjects (increasing power). With low power data no method was superior, except FFEM when associated with highly correlated covariates ($$r=0.9$$

r
=
0.9

), sSCM and PACS suffering both from selection bias. For high power data, PACS and sSCM performed similarly, both superior to FFEM. PACS is an alternative for covariate selection considering both the expected power to identify an anticipated covariate relation and the probability of prior information being correct. A proposed strategy is to use FFEM whenever the expected power to distinguish between contending models is < 80%, PACS when > 80% but < 100%, and SCM when the expected power is 100%.
Development of a Bi/Tl separation scheme for the proof of 209Bi α-decay in old mineral samples
Authors: Beu Kevin , Strub Erik , Fonseca Raúl O. C. , Greef Karin 
Published: July 2020

AbstractThe aim of this study is the development of a separation technique for geological samples, namely bismuthinites, with the goal to isolate Tl from excess amounts of Bi. This will enable an isotopic measurement of the Tl content on the ultra-trace level to identify a possible signature of Bi α-decay. The separation of Tl from other elements like e.g. Pb has been the subject of numerous studies. However, the separation of ultra-traces of Tl from bulk Bi had not yet been investigated sufficiently. This paper describes a separation technique for the system Bi/Tl for application to ultra-trace analysis. The separation technique uses a column chromatographic system, utilizing the specific redox behavior of Tl. The results show, that Tl can be successfully separated in appearance of vast excess amounts of Bi, showing recovery rates up to 96%. The developed procedure was successfully applied for the ultra-trace analysis on a number of bimuthinites using ICP-MS. On base of the results it is discussed, which prerequisites bimuthinite samples must fulfill to be suitable for the quantification of Bi alpha decay on base of the Tl isotopic ratio.
Surgical Management of Massive Irreparable Cuff Tears: Latissimus Dorsi Transfer for Posterosuperior Tears
Authors: Wieser Karl , Ernstbrunner Lukas , Zumstein Matthias A. 
Published: July 2020

AbstractPurpose of ReviewThis review aims to describe the role of the latissimus dorsi transfer (LDT) for patients with irreparable posterosuperior rotator cuff tears (RCTs).Recent FindingsHistorically, the LDT has been performed as an open (double-incision) procedure for neurologically intact, relatively young patients with irreparable posterosuperior RCTs with disabling loss of active external rotation with or without impaired active elevation. The transferred tendon reconstitutes the posterior rotator cuff and force couple, respectively and thus has the potential to function effectively as an external rotator and humeral head depressor. Long-term results of the open technique have demonstrated in the majority of patients substantial and durable improvements in shoulder function and pain relief at the 10-year benchmark. With the advancements of arthroscopic surgery, the LDT was expanded to an arthroscopically assisted procedure with promising short-term results. In addition to adequate technical performance, the success of the procedure depends on preoperative factors, such as exclusion of glenohumeral osteoarthritis and acromial acetabularization; intact or reparable subscapularis tendon; intact (or hypertrophic) teres minor muscle; adequate preoperative activity of the latissimus dorsi; and normal or mild impairment of overhead function.SummaryThe LDT (open or arthroscopically assisted) is a reliable treatment option for patients with massive, irreparable posterosuperior RCTs with disabling loss of active external rotation, with or without diminished overhead function and without advanced glenohumeral osteoarthritis. Precise patient selection is of tremendous importance in the success of the LDT.
Monoclonal gammopathy of undetermined significance (MGUS): where is the hidden danger? Definition and work-up
Authors: Jeryczynski Georg , Krauth Maria-Theresa 
Published: July 2020

SummaryMonoclonal gammopathy of undetermined significance (MGUS) is a premalignant hematological condition arising from B‑cells, characterized by the presence of monoclonal immunoglobulin production, also known as paraprotein. It is found in up to 3% of individuals over the age of 50 years. The overall progression rate is low at around 1% per year, with most patients progressing to multiple myeloma (MM). Other diseases that may arise from MGUS include non-Hodgkin lymphomas, Waldenström macroglobulinemia, amyloid light-chain (AL) amyloidosis, POEMS (polyneuropathy, organomegaly, endocrinopathy, M‑protein, skin changes), cryoglobulinemia, monoclonal gammopathy of renal significance (MGRS), and additional paraprotein-driven diseases. Every case of monoclonal gammopathy warrants careful investigation to rule out the presence of a malignant form. In recent years, paraprotein-associated conditions such as MGRS have been increasingly recognized. Accurate diagnosis and work-up of these cases require a multidisciplinary approach, and it is paramount to accurately distinguish them from true MGUS in order to prevent end-organ damage. Follow-up of MGUS should be lifelong; however, a risk-adapted approach involving the primary care setting is recommended.
Natural Intelligence and Anthropic Reasoning
Authors: Slijepcevic Predrag 
Published: July 2020

AbstractThis paper aims to justify the concept of natural intelligence in the biosemiotic context. I will argue that the process of life is (i) a cognitive/semiotic process and (ii) that organisms, from bacteria to animals, are cognitive or semiotic agents. To justify these arguments, the neural-type intelligence represented by the form of reasoning known as anthropic reasoning will be compared and contrasted with types of intelligence explicated by four disciplines of biology – relational biology, evolutionary epistemology, biosemiotics and the systems view of life – not biased towards neural intelligence. The comparison will be achieved by asking questions related to the process of observation and the notion of true observers. To answer the questions I will rely on a range of established concepts including SETI (search for extraterrestrial intelligence), Fermi’s paradox, bacterial cognition, versions of the panspermia theory, as well as some newly introduced concepts including biocivilisations, cognitive/semiotic universes, and the cognitive/semiotic multiverse. The key point emerging from the answers is that the process of cognition/semiosis – the essence of natural intelligence – is a biological universal.
Cardiac function in relation to myocardial injury in hospitalised patients with COVID-19
Authors: van den Heuvel F. M. A. , Vos J. L. , Koop Y. , van Dijk A. P. J. , Duijnhouwer A. L. , de Mast Q. , van de Veerdonk F. L. , Bosch F. , Kok B. , Netea M. G. , Hoogerwerf J. , Hoefsloot W. , Tjwa E. T. T. L. , de Korte C. L. , van Kimmenade R. R. J. , Nijveldt R. 
Published: July 2020

AbstractBackgroundPrevious studies have reported on myocardial injury in patients with coronavirus infectious disease 19 (COVID-19) defined as elevated cardiac biomarkers. Whether elevated biomarkers truly represent myocardial dysfunction is not known. The aim of this study was to explore the incidence of ventricular dysfunction and assess its relationship with biomarker analyses.MethodsThis cross-sectional study ran from April 1 to May 12, 2020, and consisted of all consecutively admitted patients to the Radboud university medical centre nursing ward for COVID-19. Laboratory assessment included high-sensitivity Troponin T and N‑terminal pro-B-type natriuretic peptide (NT-proBNP). Echocardiographic evaluation focused on left and right ventricular systolic function and global longitudinal strain (GLS).ResultsIn total, 51 patients were included, with a median age of 63 years (range 51–68 years) of whom 80% was male. Troponin T was elevated (>14 ng/l) in 47%, and a clinically relevant Troponin T elevation (10 × URL) was found in three patients (6%). NT-proBNP was elevated (>300 pg/ml) in 24 patients (47%), and in four (8%) the NT-proBNP concentration was >1,000 pg/ml. Left ventricular dysfunction (ejection fraction <52% and/or GLS >−18%) was observed in 27%, while right ventricular dysfunction (TAPSE <17 mm and/or RV S’ < 10 cm/s) was seen in 10%. There was no association between elevated Troponin T or NT-proBNP and left or right ventricular dysfunction. Patients with confirmed pulmonary embolism had normal right ventricular function.ConclusionsIn hospitalised patients, it seems that COVID-19 predominantly affects the respiratory system, while cardiac dysfunction occurs less often. Based on a single echocardiographic evaluation, we found no relation between elevated Troponin T or NT-proBNP, and ventricular dysfunction. Echocardiography has limited value in screening for ventricular dysfunction.
The intercalated disc: a mechanosensing signalling node in cardiomyopathy
Authors: Pruna Mihai , Ehler Elisabeth 
Published: July 2020

AbstractCardiomyocytes, the cells generating contractile force in the heart, are connected to each other through a highly specialised structure, the intercalated disc (ID), which ensures force transmission and transduction between neighbouring cells and allows the myocardium to function in synchrony. In addition, cardiomyocytes possess an intrinsic ability to sense mechanical changes and to regulate their own contractile output accordingly. To achieve this, some of the components responsible for force transmission have evolved to sense changes in tension and to trigger a biochemical response that results in molecular and cellular changes in cardiomyocytes. This becomes of particular importance in cardiomyopathies, where the heart is exposed to increased mechanical load and needs to adapt to sustain its contractile function. In this review, we will discuss key mechanosensing elements present at the intercalated disc and provide an overview of the signalling molecules involved in mediating the responses to changes in mechanical force.