bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nanomicelles potentiate histone deacetylase inhibitor efficacy in vitro.
Authors: Pisano Simone , Wang Xiong , Parra Jezabel Garcia , Gazze Andrea , Edwards Kadie , Feltracco Veronica , Hu Yanzhen , He Le , Gonzalez Deyarina , Francis Lewis , Conlan Robert Steven , Li Chao 
Published: October 2020

Abstract
Background. Amphiphilic block copolymers used as nanomicelle drug carriers can effectively overcome poor drug solubility and specificity issues. Hence, these platforms have a broad applicability in cancer treatment. In this study, Pluronic F127 was used to fabricate nanomicelles containing the histone deacetylase inhibitor SAHA, which has an epigenetic-driven anti-cancer effect in several tumor types. SAHA loaded nanomicelles were prepared using a thin-film drying method and characterized for size, surface charge, drug content and drug release properties. Loaded particles were tested for in vitro activity and their effect on cell-cycle and markers of cancer progression. Results. Following detailed particle characterization, cell proliferation experiments demonstrated that SAHA loaded nanomicelles more effectively inhibited the growth of HeLa and MCF-7 cell lines compared with free drug formulations. The 30nm SAHA containing nanoparticles were able to release up to 100% of the encapsulated drug over a 72h time window. Moreover, gene and protein expression analyses suggested that their cytoreductive effect was achieved through the regulation of p21 and p53 expression. SAHA was also shown to upregulate E-cadherin expression, potentially influencing tumor migration.Conclusions. This study highlights the opportunity to exploit pluronic-based nanomicelles for the delivery of compounds that regulate epigenetic processes, thus inhibiting cancer development and progression.
Parents' and medical staff's experience of adolescents with suicide-related behaviors admitted to a general hospital in China: qualitative study
Authors: Fu Xi , Yang Jiaxin , Liao Xiaoli , Shen Yidong , Ou Jianjun , Li Yamin , Chen Runsen 
Published: October 2020

Abstract
Background: Currently, there is increasing awareness of suicide-related behaviors. Mental health services are a key location for assisting people with suicide-related behaviors. However, few studies focused on the evaluation and experience of the mental health care system from families and the medical staff’s perspective in China. The study aims to explore parents' and the front-line medical staff's experience of an adolescent with suicide-related behaviors admitted to the psychiatry department of a general hospital in China.Design: Qualitative study was employed in the study. Participants were recruited from a general hospital in China characterized by high levels in the Chinese mental health system.Methods: Semi-structured in-depth interviews were conducted exploring their experience and perceptions when an adolescent was admitted to the hospital. The theme analysis method is used for data analysis.Results: Participants expressed dissatisfaction in the psychiatric department. Other barriers in their work were identified, such as the shortage of staff and difficulties in caring or communicating with patients. Besides, the imperfect treatment system also contributes to the low satisfaction of patients and their families. Two themes and six subthemes were identified: 1) staff perceives difficult to engage SRBs patients (feelings of helplessness, the need for compassion, challenges of professional self-efficacy, the recommendations to the health care service); 2) parents not satisfied with the existing hospital services (doubt the hospitalization treatment and the advice to the health care service).Conclusion: This study found that insufficient staffing and lacking of systematic professional treatment models are the major challenges. We suggest increasing the input of mental health resources to expand and train the mental health service team and establish a complete set of a treatment model for SRBs.
Respiratory syncytial virus prophylaxis for prevention of recurrent childhood wheeze and asthma: a systematic review
Authors: Quinn Lauren , Shields Michael D , Sinha Ian , Groves Helen E 
Published: October 2020

Abstract
Background: Acute bronchiolitis caused by Respiratory Syncytial Virus (RSV) has been associated with greater risk of recurrent wheezing and asthma. However, it is unclear whether this association is causal. RSV-specific monoclonal antibodies have been shown to reduce RSV-related hospitalisations in high-risk infants, but the longer term follow-up has given conflicting evidence for prevention of recurrent wheeze or asthma.Objective: We performed a systematic review and meta-analysis to determine whether monoclonal antibody prophylaxis against RSV-bronchiolitis reduces the risk of subsequent recurrent wheeze or asthma. If so, this may support the hypothesis of causality. Methods: Studies were identified via an online database search using Embase, Medline, Pubmed, Web of Science and the Cochrane Library. Manufacturers of monoclonal antibodies were contacted directly for unpublished data. The intervention of interest was RSV monoclonal antibody prophylaxis and the primary outcome measure was recurrent wheeze and/or asthma. Studies were screened according to inclusion/exclusion criteria. Included studies were evaluated for quality and assessed for bias independently by 3 reviewers using the ‘Grading of Recommendations Assessment, Development and Evaluation’ (GRADE) approach. Results were extracted into 2x2 outcome tables and a meta-analysis carried out producing forest plots based on relative risk. Heterogeneity was assessed using the I2 statistic. Results: The search identified 141 articles, which, after screening resulted in eight studies (2 randomised controlled trials), thus including 11,195 infants in the meta-analysis. The overall result demonstrated a non-statistically significant reduction in relative risk of developing recurrent wheeze or asthma (RR 0.60; 95% CI 0.31 to 1.16). Study quality was generally low with evidence of publication bias and statistical heterogeneity. However, sub-group analysis excluding studies deemed to be ‘very low’ quality showed a relative risk of 0.42 (95% CI: 0.22 to 0.80, p = 0.008). A further sub-group analysis for infants aged 32 to <36 weeks showed a statistically significant relative risk of 0.35 (95% CI: 0.14 to 0.86, p = 0.02). Discussion: We did not identify an overall statistically significant benefit. However, our two subgroup analyses did find statistically significant benefits of monoclonal antibody therapy on the risk of recurrent wheeze and asthma. The main limitation of this study is the lack of high quality randomised controlled trials, highlighting the need for more research in this field.
Prevalence and Correlates of Maternal Early Stimulation Behaviors during Pregnancy in Northern Ghana: A Cross-Sectional Survey
Authors: Mackness Jessica , Gallis John A , Owusu Raymond Kofi , Ali Mohammed , Abubakr-Bibilazu Safiyatu , Adam Haliq , Aborigo Raymond , Awoonor-Williams John Koku , Lillie Margaret , McEwan Elena , Hembling John , Vasudevan Lavanya , Baumgartner Joy Noel 
Published: October 2020

Abstract
Background: Per UNICEF’s Nurturing Care Framework, early child development (ECD) begins during pregnancy and many lower-resource settings need data to inform their programs for optimal child development. The maternal-fetal relationship can be partly examined via a series of bonding activities called early stimulation behaviors (ESB). This study describes early stimulation behaviors and the associated correlates among pregnant women in Ghana. Methods: This cross-sectional study used data from a cluster-randomized trial in two districts of Northern Ghana. A total of 374 pregnant women were enrolled at baseline and administered a pre-intervention survey. Communication-related early stimulation behaviors was the primary outcome which was evaluated using three maternal-fetal bonding activities; did the woman self-report touching and/or talking, singing, and/or talking about family to her belly. A generalized estimating equation modified Poisson model was used for the bivariate and multivariable analysis.Results: About half of the participants reported performing communication-related ESB during pregnancy frequently or sometimes. Bivariate analysis revealed that negative life experiences including higher rates of emotional, physical and sexual intimate partner violence (IPV) and having moderate to severe depressive symptoms were associated with women performing early stimulation behaviors more often. In the multivariable model, physical intimate partner violence remained significantly associated with early stimulation behaviors. Conclusion: Research on early stimulation behaviors is still in a nascent phase. It is unclear why our results revealed an association between intimate partner violence and early stimulation behaviors; this could reflect a coping mechanism for the expectant mother. Further research is needed to better understand this association and explore potential long-term impacts of early stimulation behaviors during pregnancy on child development.Trial registration: Clinical Trials # NCT03665246, September 11, 2018
Executive dysfunctions mediate between altered sensory processing and daily activity performance in older adults
Authors: Yeger Batya Engel , Rosenblum Sara 
Published: October 2020

Abstract
Background: Sensory processing is essential for the interaction with the environment and for adequate daily function. Sensory processing may be deteriorated in the elderly and restrict daily activity performance. Another factor impacted by aging which significantly affects daily activities is Executive functions (EF). Yet, most studies refer separately to the impacts of sensory processing or EF on daily activities and use clinical evaluations that do not necessarily reflect functional restrictions in real life. This study aims to describe the prevalence of altered sensory processing in the elderly as expressed in daily life scenarios and explore whether EF mediate between altered sensory processing and daily activity performance in older adults.Methods: This cross-sectional study included 167 healthy and independently functioning people living in the community and aged 65 years and older. Participants who had sufficient cognitive status and no symptoms of depression completed a demographic and health-status questionnaire, the Adolescent/Adult Sensory Profile, the Behavior Rating Inventory of Executive Function–Adult Version and the Daily Living Questionnaire.Results: Altered sensory processing, and mainly by the reduced ability to register and modulate sensory input from daily environment, are prevalent in older adults. Their impacts on daily activity performance are mediated by executive dysfunctions.Conclusions: Executive dysfunctions may worsen the negative effects of altered sensory processing on daily activity performance in older adults. The interaction between executive functions and sensory processing should receive growing attention in intervention and prevention programs for older adults, with the emphasis on their expressions in peoples’ real life context.
Dysregulated Bile Acid Receptor-mediated Signaling and IL-17A Induction are Implicated in Diet-associated Hepatic Health and Cognitive Function
Authors: JENA PRASANT KUMAR , Sheng Lili , Nguyen Michelle , Lucente Jacopo Di , Hu Ying , Li Yongchun , Maezawa Izumi , Jin Lee-way , Wan Yu-Jui 
Published: October 2020

Abstract
Background: Chronic consumption of high sugar and high fat diet associated with liver inflammation and cognitive decline. This paper tests a hypothesis that the development and resolution of diet-induced nonalcoholic fatty liver disease (NAFLD) has an impact on neuroplasticity and cognition. Methods: C57BL/6 wild-type mice were fed with either a healthy control diet (CD) or a fructose, palmitate, and cholesterol (FPC)-enriched diet since weaning. When mice were 3-months old, FPC diet-fed mice were randomly assigned to receive either FPC-enriched diet with or without 6% inulin supplementation. At 8 months of age, all three groups of mice were euthanized followed by analysis of inflammatory signaling in the liver and brain, gut microbiota, and cecal metabolites. Results: Our data showed that FPC diet intake induced hepatic steatosis and inflammation in the liver and brain along with elevated RORγ and IL-17A signaling. Accompanied by microglia activation and reduced hippocampal long-term potentiation, FPC diet intake also reduced postsynaptic density-95 and brain derived neurotrophic factor, whereas inulin supplementation prevented diet-reduced neuroplasticity and the development of NAFLD. In the gut, FPC diet increased Coriobacteriaceae and Erysipelotrichaceae, which are implicated in cholesterol metabolism, and the genus Allobaculum, and inulin supplementation reduced them. Furthermore, FPC diet reduced FXR and TGR5 signaling, and inulin supplementation reversed these changes. Untargeted cecal metabolomics profiling uncovered 273 metabolites, and 104 had significant changes due to FPC diet intake or inulin supplementation. Among the top 10 most affected metabolites, FPC-fed mice had marked increase of zymosterol, a cholesterol biosynthesis metabolite, and reduced 2,8-dihydroxyquinoline, which has known benefits in reducing glucose intolerance; these changes were reversible by inulin supplementation. Additionally, the abundance of Barnesiella, Coprobacter, Clostridium XIVa, and Butyrivibrio were negatively correlated with FPC diet intake and the concentration of cecal zymosterol but positively associated with inulin supplementation, suggesting their benefits. Conclusion: Taken together, the presented data suggest that diet alters the gut microbiota and their metabolites, including bile acids. This will subsequently affect IL-17A signaling, resulting in systemic impacts on both hepatic metabolism and cognitive function.
Resilience, Occupational Burnout, and Parenting Stress in Nurses Caring for COVID-2019 Patients
Authors: Bashirian Saeid , Bijani Mostafa , Borzou Seyed Reza , Khazaei Salman , Mohammadi Fateme 
Published: October 2020

Abstract
Background: Caring of patients with Corona Virus Infection Disease (COVID 2019) may have had an impact on the occupational burnout, resilience, and parenting of the nurses. Purpose: Evaluation occupational burnout, resilience, and parenting stress in nurses caring for COVID 2019patients.Methods: The present study is a cross-sectional study Base on Strengthening the Reporting of Observational Studies in Epidemiology Statement (STROBE). 420 nurses cared for COVID 2019 patients in 5 hospitals selected via convenience sampling. Each participant completed the scales online. Data were analyzed in SPSS v. 22.Results: Occupational burnout has a negative correlation with resilience and a positive correlation with parenting stress and can predict 61.32% of changes in the occupational burnout variance of nurses. Conclusion: the resilience, parenting stress, marital status, number of children, employment status, and gender predicated High percentage of the nurses’ occupational burnout variance. The nurse managers should use these findings to provide appropriate environments for nurses, that to develop more comprehensive plans for support of nurses for the current and future crises.
Validity and reliability of the Portuguese version of the modified Migraine Disability Assessment
Authors: Ferreira Pedro , Luzeiro Isabel , Lopes Margarida , Jorge André , Silva Bruno , Ferreira Lara 
Published: October 2020

Abstract
Background
Migraine Disability Assessment Scale (MIDAS) is a useful tool to measure headache-related disability. Modified MIDAS with 4-week recall period reduces recall bias and improves accuracy of the results. This study aimed at validating mMIDAS in Portuguese.
Methods
Studied population consisted of adult migraine patients attending a headache outpatient clinic. Reliability was assessed by internal consistency and reproducibility in a 3-week test-retest. Content validity was evaluated by two expert panels. Construct validity was tested by comparing mMIDAS-P index in socioeconomic and clinical patient groups and scale unidimensionality was evidenced by factor analysis. Criterion validity was tested using EQ-5D-5L and HADS.
Results
92 patients, 88% female, mean age of 44 years, participated. They had, in average, 9.7 headache days in previous month, pain averaging 7.5/10. About 69.9% were on a migraine prophylactic treatment, and 42.4% had severe disability; 29.4% and 13.0% showed, respectively, moderate/severe anxiety and depression. Content validity showed that mMIDAS-P is simple and clinically useful. It did not show to be determined by patient’s sociodemographic characteristics and it was correlated with depression scale and EQ-5D-5L. Test-retest demonstrated high reproductive reliability and good internal consistency.
Conclusion
mMIDAS-P is valid and reliable. We strongly recommend it for clinical and research use.
CellRank for directed single-cell fate mapping
Authors: Lange Marius , Bergen Volker , Klein Michal , Setty Manu , Reuter Bernhard , Bakhti Mostafa , Lickert Heiko , Ansari Meshal , Schniering Janine , Schiller Herbert , Pe'er Dana , Theis Fabian 
Published: October 2020

Abstract
Computational trajectory inference enables the reconstruction of cell-state dynamics from single-cell RNA sequencing experiments. However, trajectory inference requires that the direction of a biological process is known, largely limiting its application to differentiating systems in normal development. Here, we present CellRank (https://cellrank.org) for mapping the fate of single cells in diverse scenarios, including perturbations such as regeneration or disease, for which direction is unknown. Our approach combines the robustness of trajectory inference with directional information from RNA velocity, derived from ratios of spliced to unspliced reads. CellRank takes into account both the gradual and stochastic nature of cellular fate decisions, as well as uncertainty in RNA velocity vectors. On data from pancreas development, we show that it automatically detects initial, intermediate and terminal populations, predicts fate potentials and visualizes continuous gene expression trends along individual lineages. CellRank also predicts a novel dedifferentiation trajectory during regeneration after lung injury, which we follow up experimentally by confirming the existence of previously unknown intermediate cell states.
Evidence-informed decision about (de-)implementing return-to-work coordination – a case study
Authors: Tikka Christina , Verbeek Jos , Hoving Jan , Kunz Regina 
Published: October 2020

Abstract
BackgroundCoordination of return-to-work (RtW) with the aim to decrease sick leave in many countries. Compared to usual care, a Cochrane review found RtW coordination to have no considerable effect on sick leave. The aim of the study is to find out how the evidence from this review can be used for decisions about (de-)implementing RtW coordination in a country specific setting, using Finland as an example. MethodsWe conducted a systematic literature search and online survey with two groups of experts to collect data on the similarity of RtW interventions in Finland and those evaluated in the Cochrane review. Using content analysis methods we analysed how comparable the interventions in the Cochrane review are to Finish practice. We modelled the costs of RtW coordination compared to usual care for Finland. We used the criteria of the evidence-to-decision framework to draw conclusions about (de-)implementing RtW coordination in Finland. ResultsWe included 7 documents from the literature search and received survey data from 10 survey participants. RtW coordination included, both in Finland and in the review, at least one face-to-face meeting between the physician and the worker, a workers’ needs assessment, and an individual RtW plan and its implementation. Usual care focuses on medical treatment and may include general RtW advice. RtW coordination would be cost saving if it decreases sick leave with at least two days compared to usual care. The evidence in the Cochrane review was mainly of low certainty and the effect sizes had relatively wide confidence intervals. Only a new, high quality and large RCT can decrease the current uncertainty but this is unlikely to happen. The evidence-to-decision framework did not provide arguments for further implementation nor for de-implementation of the intervention. ConclusionsInterventions evaluated in the Cochrane review are similar to RtW coordination and usual care interventions in Finland. Considering all EtD framework criteria, including certainty of the evidence and costs, de-implementation of RtW coordination interventions in Finland seems unnecessary. Better evidence about the costs and stakeholders’ values regarding RtW coordination is needed to improve decision making.
Radiological and Long-Term Patient-Reported Outcome after Stabilization of Traumatic Thoraco-Lumbar Spinal Fractures using an Expandable Vertebral Body Replacement Implant.
Authors: Lang Siegmund , Neumann Carsten , Schwaiger Christina , Voss Andreas , Alt Volker , Loibl Markus , Kerschbaum Maximilian 
Published: October 2020

Abstract
BackgroundA combined posterior and anterior stabilization was found to offer a better restoration of the sagittal profile of thoracolumbar fractures in comparison to posterior-only stabilization. Data on restoring the bisegmental kyphotic endplate angle (BKA) and on patient-reported outcome measures (PROM) is sparse.Patients and Methods136 patients with traumatic thoraco-lumbar spinal fractures were treated with an expandable VBR implant (Obelisc™, Ulrich Medical, Germany). in our trauma department between 2001 and 2015. Radiological data acquisition was completed pre- and postoperatively, after at least six months and approximately two years after surgery. The BKA was measured at each follow-up. The patient related outcome was evaluated prospectively.Results117 (31 female, 86 males; age: 52.7 ± 16.5 years) patients were included in the study. 64 patients (54.7% of included patients; age: 53.2 ± 14.8 years) completed the PROM questionnaires and therefore were assigned to the study group. An A4 fracture was seen in 99 cases (84.6%) and 85 (72.6%) fractures were located at the lumbar spine. Intraoperative reduction of the BKA in 117 patients was 6.6 ± 8.2° (p < 0.01). A significant loss of reduction of 2.6 ± 4.2° at the first follow-up (t1) and of 4.4 ± 6.6° at the second follow-up (t2) was measured (each p < 0.05). The consolidation rate was 95.7%. The total revision rate was 5.1%. 64 patients (54.7% of included patients; age: 53.2 ± 14.8 years) completed the PROM questionnaires and therefore were assigned to the study group. Mean time between index surgery and completion of the PROM questionnaire was 109.4 ± 44.6 months. Mean ODI of was 28.4 ± 17.6, the mean EQ-5D VAS reached 57.9 ± 2.5 points. The cohort showed significantly lower SF-36 values compared to a healthy reference population (each p < 0.05).ConclusionTreatment of traumatic thoraco-lumbar spinal fractures using an expandable VBR implant is an effective and safe procedure. A significant operative correction of the BKA could be achieved. Although the initial reconstruction of the BKA did not consist over time in the study group, satisfactory PROMs were found.