bluuebunny/crossref_metadata_2025_split huggingface dataset:
WITHDRAWN: Do provider behaviors influence client satisfaction of family planning services in Togo? Evidence from a cross-sectional facility survey
Authors: Dougherty Leanne , Spielman Kathryn , Silva Martha 
Published: December 2020

Abstract
BackgroundStrengthening quality of family planning care is a key objective in Togo to improve maternal and reproductive health. Structural attributes or inputs to care, and process attributes or content of care, including providers’ interaction with clients, are key factors determining quality of care. Client satisfaction with family planning services is linked to contraceptive uptake and continuation, yet the relationship between quality of care elements and client satisfaction of family planning services has not been assessed in Togo, particularly process factors related to client-provider interactions. MethodsWe conduct a secondary analysis using data from a facility-based survey. The survey was conducted in August 2016 in six health districts of Lomé Togo, including a facility audit (N=16), client observations (N=1096) and client exit interviews with women of reproductive age (N=1089). We used multi-variable logistic regression to assess the association between factors related with service structure and process, including provider-client interaction and client perception of provider treatment as a proxy for client satisfaction.ResultsWe did not find a relationship between structural attributes of quality and client perception of provider treatment. Among process attributes, we found that several behaviors related to interpersonal skills, including encouraging clients to ask questions and asking clients to describe any concerns they have with their method, and providers use of visual aids were significantly associated with client perception of provider treatment. ConclusionThe quality of care a woman receives when seeking to adopt or continue family planning methods is essential to improving use of family planning service. Family planning programs must address provider related behaviors that may inhibit the uptake and continuation of contraceptive use, and strengthen inter-personal skills, which may improve client perception of provider treatment with services and facilitate their continued use.
An improved biolistic delivery and analysis method for evaluation of DNA and CRISPR-Cas delivery efficacy in plant tissue
Authors: Miller Kyle , Eggenberger Alan , Lee Keunsub , Liu Fei , Kang Minjeong , Drent Madison , Ruba Andrew , Kirscht Tyler , Wang Kan , Jiang Shan 
Published: December 2020

Abstract
Biolistic delivery is widely used for genetic transformation but inconsistency between bombardment samples for transient gene expression analysis often hinders quantitative analyses. We developed a methodology to improve the consistency of biolistic delivery results by using a double-barrel device and a cell counting software. The double-barrel device enables a strategy of incorporating an internal control into each sample, which significantly decreases variance of the results. The cell counting software further reduces errors and increases throughput. The utility of this new platform is demonstrated by optimizing conditions for delivering DNA using the commercial transfection reagent TransIT®-2020. In addition, the same approach is applied to test the efficacy of CRISPR-Cas9-mediated gene editing. The novel combination of the bombardment device and analysis method allows simultaneous comparison and optimization of parameters in the biolistic delivery. The platform developed here can be broadly applied to any target samples using biolistics, including animal cells and tissues.
Construction and Validation of the Prognostic Model for Patients With Neuroendocrine Cervical Carcinoma: A Competing Risk Nomogram Analysis
Authors: Jiang Ai-Guo , Cai Xu 
Published: December 2020

Abstract
Purpose: Neuroendocrine cervical carcinoma (NECC) is an uncommon malignancy of the female reproductive system. This study aimed to evaluate the cancer-specific mortality and to construct prognostic nomograms for predicting the survival of patients with NECC.Methods: we assembled the patients with NECC diagnosed between 2004 to 2015 from the Surveillance, Epidemiology, and End Results (SEER) database. Meanwhile, we identified other patients with NECC from the Wenling Maternal and Child Health Care Hospital between 2002 to 2017. Fine and Gray’s test and Kaplan-Meier methods were used to evaluate cancer-specific mortality and overall survival (OS) rates, respectively. Nomograms were constructed for predicting cancer-specific survival (CSS) and OS for patients with NECC. The developed nomograms were validated both internally and externally.Results: a total of 894 patients with NECC extracted from the SEER database, then classified them into the training cohort (n=628) and the internal validation cohort (n=266). Besides, 106 patients from the Wenling Maternal and Child Health Care Hospital served as an external validation cohort. Nomograms for predicting CSS and OS were constructed on clinical predictors. The validation of nomograms was calculated by calibration curves and concordance indexes (C-indexes). Furthermore, the developed nomograms presented higher areas under the receiver operating characteristic (ROC) curves when compared to the FIGO staging system.Conclusions: we established the first competing risk nomograms to predict the survival of patients with NECC. Such a model with excellent performance could be a practical tool for clinicians.
Association Between miR-30a/SNAI1 Gene Polymorphisms and the Risk of Diabetic Kidney Disease
Authors: Yang CaiLian , Tong Tao , Sun MingFang , Zhou Bo 
Published: December 2020

Abstract
Background: Diabetic kidney disease is a leading cause of end-stage kidney disease worldwide, its incidence is still increasing and the precise mechanism is not yet fully understood.This study aimed to investigate the possible association of miR-30a and its potential target gene, SNAI1, polymorphisms with diabetic kidney disease in patients with type 2 diabetes mellitus. Methods: This case-control is study included 240 type 2 diabetes mellitus patients with diabetic kidney disease and 280 patients without diabetic kidney disease. Genotyping of miR-30a and SNAI1 polymorphisms were performed by allelic discrimination assay with Taq-Man-MGB probes. Result: The results demonstrated that the CC genotype of rs2222722 in miR-30a was associated with an increased risk of diabetic kidney disease in Chinese type 2 diabetes mellitus patients (OR = 2.81, 95% CI 1.58-4.97, p < 0.001). Stratified analyses revealed that the miR-30a CC genotype was strongly associated with an increased risk of diabetic kidney disease in subjects who were young (< 65 years), male, and had a low body mass index, as well as those with poor glycemic control. What’s more, CC genotype carriers were more likely to exhibit decreased estimated glomerular filtration rate levels and urinary protein production. In addition, the GG genotype of SNAI1 was also associated with the development of diabetic kidney disease, and the risk was 1.73 times higher than that in AA+AG genotype carriers (95% CI 1.01-2.96, p = 0.046). Multiple logistic regression analysis showed that the miR-30a CC and SNAI1 GG genotypes were indispensable and dangerous contributing factors to the development of diabetic kidney disease. Conclusion: The CC genotype of miR-30a rs2222722 and GG genotype of SNAI1 rs1543442 might be associated with diabetic kidney disease risk in Chinese type 2 diabetes mellitus patients.
Primiparous and Multiparous Ewes Undernourished During Gestation: A Study on Mother-Lamb Bond at Birth and at 3 Months of Age
Authors: de Melo Aline Freitas , Pérez-Clariget Raquel , Terrazas Angélica , Ungerfeld Rodolfo 
Published: December 2020

Abstract
The aims were to compare ewe-lamb behaviours between primiparous (PRI) and multiparous (MUL) undernourished grazing ewes at birth and at 3 months of age, and to determine if mothers’ parity affects milk yield and composition, and lambs’ body weight (BW). Food availability restricted the nutritional requirements from day 30 to day 143 of gestation. The PRI ewes had lower BW than MUL during gestation, and their lambs tended to vocalize less times until their first suckle. PRI ewes displayed a lower frequency of acceptance behaviours toward the alien lamb than toward their own lamb, but MUL did not. Whilst PRI ewes emitted a greater number of high-pitched bleats toward the alien lambs than toward their own lamb, MUL did not. PRI ewes produced less milk than the MUL ewes. The heart rate was greater in lambs reared by MUL ewes than by PRI. Although PRI ewes had a lower BW throughout the gestation, this difference was stable and did not affect the establishment of the ewe-lamb bond at birth or at 3 months postpartum. At least under nutritionally restricted conditions during gestation, inexperienced mothers appeared to have a shorter sensitivity period of maternal responsiveness than experienced mothers.
Fully Coupled Stochastic Geomechanical-Geochemical - Reservoir Modeling for Fracture Reservoirs
Authors: zeqiraj dulian Dushan 
Published: December 2020

Abstract
Carbonate reservoirs contain a significant portion of the world's oil reserves. The uptake of oil from these reservoirs is difficult due to the complex hydro-thermo-geomechanical-geochemical processes. In recent years many efforts have been made to model these processes in fractured rocks. A few studies have been done on the stochastic nature of these processes. Geomechanical models need to take into account the reservoir rock deformations that are the result of the pores and fracture pressure, while geochemical models deal with the chemical reactions that take place into fracture and matrix with their principal processes such dissolution and precipitation. In this paper we will make the modeling from the stochastic point of view of these processes by making a model to deal with such parameters and coefficients that are stochastic, seeing them as random variables. In our knowledge this is the first work of this kind. From the geomechanical point of view we will use the stochastic nature of the Biot linear model with uncertain coefficients, while for the stochastic geochemical model we will use the Langrazhian formulation.
A Man with Simultaneous Occurrence of Acute Lower Extremity Arterial Embolization and Acute Myocardial Infarction: A Case Report
Authors: Lu Liangyuan , Xue Yanyan , Han Yalei , Bao Tongxin , Xue Jing , Yang Zhanmin 
Published: December 2020

Abstract
Background: Spontaneous co-occurrence of acute myocardial infarction (AMI) and acute lower extremity arterial embolism (ALEAE) has rarely been reported.Case presentation: A 44-year-old male with a history of 4 years of type1 diabetes was admitted to hospital when he suddenly experienced severe pain in his right lower limb and felt tightness in the left anterior chest area. Ultrasonography revealed distal occlusion of the right superficial femoral artery. ECG showed acute anterior interstitial myocardial infarction. After conservative treatment for 2 days, the patient had severe necrosis of lower limbs and secondary injury of multiple organs. Hemodialysis and heparin anticoagulant therapy were performed before amputation. Twelve days after the operation, the patient's condition was stable and he was transferred out of ICU.Conclusions: Emergency amputation and multidisciplinary approaches may offer a chance for survival if patents lost the opportunity for early treatment.
Associations of Insulin Resistance and Beta Cell Function with Abnormal Lipid Profile in Newly Diagnosed Diabetes: A Cross-sectional Study
Authors: Tang Xiaohan , Yan Xiang , Zhou Houde , Huang Gan , Niu Xiaohong , Jiang Hongwei , Su Heng , Yang Xilin , Li Xia , Zhou Zhiguang 
Published: December 2020

Abstract
Background: Abnormal lipids are strong predictive factors of cardiovascular disease (CVD) in both type 1 diabetes mellitus (T1DM) and type 2 diabetes mellitus (T2DM). However, the potential associations of insulin resistance (IR) and beta cell function (BCF) in diabetes and abnormal lipids, i.e. high triglyceride (TG), low high-density lipoprotein cholesterol (HDL-C) and high low-density lipoprotein cholesterol (LDL-C) are not fully understood. In this study, we aim to explore whether decreased BCF and increased IR in newly diagnosed T1DM or T2DM are associated with abnormal lipids.Methods: Clinical and laboratory data were collected from 16334 adults with newly diagnosed diabetes in this cross-sectional study. Types of diabetes were diagnosed based on clinical characteristics and diabetes-related biochemical measurement results. Homeostasis model assessment were used to estimate IR and BCF. Restricted cubic spline and binary logistic regression were used to examine the associations of IR or BCF and abnormal lipids in T1DM and T2DM, respectively. Results: High TG, low HDL-C and high LDL-C accounted for 49.7%, 47.7% and 59.2%, respectively. In multivariable analysis, high IR was associated with increased risk of high TG (Odds ratios (ORs) of homeostasis model assessment of insulin resistance (HOMA2-IR) ≥2, ≥1-<2 vs <1: 4.77, 95% CI 2.69-8.57; 2.31, 95% CI 1.54-3.47, p for trend < 0.001) in T1DM and was associated with elevated risk of high TG, low HDL-C and high LDL-C (all p for trend <0.01) in T2DM. Low BCF, i.e., homeostasis model assessment of beta-cell function (HOMA2-B) <30 versus ≥30, was associated with marginally increased risk of high TG (OR 1.42, 95% CI 0.98-2.10, p = 0.07) in T1DM and associated with increased risk of high TG (OR 1.21, 95% CI 1.09-1.34, p <0.001) and high LDL-C (OR 1.23, 95% CI 1.12-1.36, p <0.001) in T2DM.Conclusions: In patients with newly diagnosed diabetes, high IR and low BCF had different associations with risk of dyslipidemia in T1DM and T2DM, suggesting that early treatment that improve IR or BCF may have a benefit for abnormal lipid metabolism.
Aerobic Exercise Training Effects on Omentin-1, Insulin Resistance and Lipid Profile Among Male Smokers
Authors: Mousavi Seyed Maysam , Heidarianpour Ali , Tavassoli Hassan 
Published: December 2020

Abstract
Background: Omentin-1 is a recently discovered circulating adipokine that plays a crucial role in modulating insulin resistance and diabetes. We investigated the effect of eight weeks of aerobic exercise training on serum omentin-1, insulin resistance and lipid profile in the smokers and non-smokers with normal weight. Methods: Nineteen male healthy and twenty male smokers were randomly assigned into healthy control group (C), healthy exercise group (E), control smoker group (CS) and exercise smoker group (ES). Exercise groups participated in an eight-week aerobic exercise training program (three times a week, 20-35 min per session at 55%-70% of maximum heart rate). Serum omentin-1 and insulin values were determined by ELISA and Homeostatic Model Assessment for Insulin Resistance (HOMA-IR), glucose and lipid profile was measured before and after the intervention. Paired samples t-test, one-way analysis of variance (One-way ANOVA) and post-hoc Tukey test were applied to analyze the data (p<0.05).Results: Aerobic exercise improved both serum omentin-1 and high lipoprotein cholesterol (HDL-C) in the exercise groups (p<0.05). Also, Exercise training reduced insulin, blood sugar, HOMA-IR, total cholesterol (TC) and low-density lipoprotein cholesterol (LDL-C) levels (p<0.05). Omentin-1 was significantly correlated with insulin (r=-0.40, P=0.01), HOMA-IR (r=-0.38, P=0.04), TG (r=-0.40, P=0.01), TC (r=-0.49, P=0.02), LDL-C (r=-0.70, P=0.02) and HDL-C (r=0.55, P=0.03).Conclusion: The findings suggest that aerobic exercise-induced changes in omentin-1 in exercise trained smokers may be associated with the beneficial effects of exercise on reduced insulin resistance and lipid profile.
Identification of biomarkers complementary to homologous recombination deficiency for improving the clinical outcome of ovarian serous cystadenocarcinoma
Authors: Shi Zhiwen , Zhao Qingguo , Lv Bin , Qu Xinyu , Han Xiao , Qiu Junjun , Wang Hongyan , Hua Keqin 
Published: December 2020

Abstract
Background: Ovarian cancer patients with tumors positive for homologous recombination deficiency (HRD) would benefit from PAPR inhibitor (PAPRi)therapy. However, patients with HRD-positive tumors account for less than 50% of the whole cohort. So new biomarkers still need to be developed. The aim of this study was to identify biomarkers complementary to HRD for improving the clinical outcome of ovarian serous cystadenocarcinoma (OSC).Methods: The HRD score was established from SNP array data of OSC cohort from TCGA. The genomic landscape and transcriptome data of patients with different HRD scores were analyzed in order to identify biomarkers complementary to HRD. The candidate biomarker was validated in vivo and in vitro experiments.Results: Based on the data from the SNP array and somatic mutation profiles in the OSC patients’ genome, we found that high frequency of actionable mutations existed in NF1 and CDK12 in patients with HRD-negative tumors. We then leveraged the gene expression profiles to screen out CXCL11 expression that was associated with the HRD score and could be used as a predictor of survival outcome. Further comprehensive analysis of the tumor immune microenvironment (TIME) revealed that CXCL11 expression was closely correlated with cytotoxic cells, neoantigen load and immune checkpoint blockade (ICB). Finally, in vivo and in vitro experiments showed that PAPRi increased the expression of CXCL11. Conclusions: Collectively, our study not only provides biomarkers of ovarian cancer complementary to the HRD score but also introduced a potential new perspective for identifying cancer biomarkers associated with genomic instability.
NIR Laser Responsive Nanoparticles for Ovarian Cancer Targeted Combination Therapy with Dual-modal Imaging Guidance
Authors: Zhao Jiawen , Zhang Liang , Qi Yingjie , Liao Kui , Wang Zhigang , Wen Ming , Zhou Di 
Published: December 2020

Abstract
Background: Multifunctional nanoparticles with targeted therapeutic function and diagnostic-imaging are of great interest in the domain of precision therapy. The synergetic photothermal therapy (PTT)/chemotherapy with the guidance of CT/PA imaging is greatly expected to precisely kill cancer cells.Results: The synthesized FBPD NPs not only own excellent CT/PA imaging contrast increase ability but also reveal superior tumor-killing efficiency. The prepared FBPD NPs revealed excellent dispersity, great stability, outstanding optical properties. The release effciency of Dox in FBPD NPs after laser irradiation was achieved 86.7%. Meanwhile, Liquid-gas phase conversion of perfluoropentane (PFP) induced by PTT was discovered and verified, which significantly enhanced the drug release efficiency in the tumor region. Furthermore, a photosensitizer irradiated by PTT engenders heat toward tumor region. According to CCK-8 assay, the cell viability of FBPD NPs + laser group reached 8.9%. In addition, in the cytotoxicity assay in vivo, the tumors in the FBPD NPs + laser group were completely ablated. These results revealed whether in vitro or in vivo, assessments on tumor-bearing mice proved that the precise inhibiting efficiency of PTT combined with FBPD NPs, which played a significant role in the inhibition of tumor growth.Conclusion: The FBPD NPs was successfully fabricated, realizing CT/PA imaging-guided combination therapy against ovarian cancer. The unique nanoparticles with multiple abilities paves a emerging way toward precise treatment.
WRAP-based nanoparticles for siRNA delivery: A SAR study and a comparison with lipid-based transfection reagents.
Authors: Konate Karidia , Josse Emilie , Tasic Milana , Redjatti Karima , Aldrian Gudrun , Deshayes Sébastien , Boisguérin Prisca , Vives Eric 
Published: December 2020

Abstract
Recently, we designed novel amphipathic cell-penetrating peptides, called WRAP, able to transfer efficiently siRNA molecules into cells. In order to gain more information about the relationship between amino acid composition, nanoparticle formation and cellular internalization of these peptides composed of only three amino acids (leucine, arginine and tryptophan), we perform a structure activity relationship (SAR) study. First, we compared our WRAP1 and WRAP5 peptides with the C6M1 peptide also composed of the same three amino acids and showing similar behaviors in siRNA transfection. Afterwards, to further define the main determinants in the WRAP activity, we synthesized 13 new WRAP analogues harboring different modifications like the number and location of leucine and arginine residues, the relative location of tryptophan residues, as well as the role of the α-helix formation upon proline insertions within the native WRAP sequence. After having compare the ability of these peptides to form peptide-based nanoparticles (PBNs) or not using different biophysical methods (circular dichroism, dynamic light scattering, gel shift assay) and to induce a targeted gene silencing (luciferase assay) in cells, we were able to establish the main sequential requirements of the amino acid composition of the WRAP peptides to maintain a good siRNA transfection efficacy. In addition, upon measuring the WRAP-based siRNA transfection ability into cells compared to several non-peptide transfection agents available on the markets, we confirmed that WRAP peptides induced an equivalent level of targeted gene silencing but in most of the cases with significant lower cell toxicity as clearly shown in clonogenic assays.
Analysis of Tracheal Intubation in Out-of-Hospital Helicopter Emergency Medicine Recorded by Video Laryngoscopy.
Authors: Knapp Jürgen , Eberle Bettina , Bernhard Michael , Theiler Lorenz , Pietsch Urs , Albrecht Roland 
Published: December 2020

Abstract
Objective. Tracheal intubation remains the gold standard of airway management in emergency medicine and maximizing safety, intubation success and especially first-pass intubation success (FPS) in these situations is imperative.Methods. We conducted a prospective observational study on all twelve helicopter emergency medical service (HEMS) bases of the Swiss Air Rescue, between February 15, 2018, and February 14, 2019. All 428 patients on whom out-of-hospital advanced airway management was performed by the HEMS crew were included. The C-MAC video laryngoscope was used as primary device for tracheal intubation. Intubation procedures were recorded by the video laryngoscope and precise time points were recorded to verify the time necessary for each attempt and the overall procedure time until successful intubation. The videos were further analysed for problems and complications during airway management by an independent reviewer. Additionally, a questionnaire about the intubation procedure, basic characteristics of the patient, as well as circumstances and environmental factors, and the level of experience in airway management of the provider had to be filled out. Main outcome measures were FPS of tracheal intubation, overall success rate, overall intubation time, problems and complications of video laryngoscopy.Results. FPS rate was 87.6% and overall success rate 98.6%. Success rates, overall time to intubation, and subjective difficulty were not associated to the providers’ expertise in airway management. In patients undergoing CPR FPS was 84.8%, in trauma patients 86.4% and in non-trauma patients 93.3%. FPS in patients with difficult airway characteristics, facial trauma/burns or obesity ranges between 87 and 89%. Performing airway management indoors or inside an ambulance resulted in a significantly higher FPS of 91.1% compared to outdoor locations (p<0.001). Direct solar irradiation on the screen, fogging of the lens, and blood on the camera significantly impaired FPS. Several issues for further improvements in the use of video laryngoscopy in the out-of-hospital setting and for quality control in airway management were identified.Conclusion. Airway management using the C-MAC video laryngoscope with Macintosh blade in a group of operators with mixed experience showed high FPS and overall rates of intubation success. Video recording emergency intubations may improve education and quality control.