bluuebunny/crossref_metadata_2025_split huggingface dataset:
An engaged approach to exploring issues around poverty and mental health: A reflective evaluation of the research process from researchers and community partners involved in the DeStress study
Authors: Thomas Felicity , Hansford Lorraine , Wyatt Katrina , Byng Richard , Coombes Karen , Finch Jenna , Finnerty Kirsty , Ford Joe , Guppy Keith , Guppy Rachel , Hughes Susanne , McCabe Rose , Richardson Hilary , Roche Debbie , Stuteley Hazel 
Published: May 2020

AbstractBackgroundInvolving patients, service users, carers and members of the public in research has been part of health policy and practice in the UK for the last 15 years. However, low‐income communities tend to remain marginalized from the co‐design and delivery of mental health research, perpetuating the potential for health inequalities. Greater understanding is therefore needed on how to meaningfully engage low‐income communities in mental health research.ObjectivesTo explore and articulate whether and how an engaged research approach facilitated knowledge coproduction relating to poverty and mental distress.SettingA reflective evaluation of community and researcher engagement in the DeStress study that took place in two low‐income areas of South‐west England.DesignReflective evaluation by the authors through on‐going feedback, a focus group and first‐person writing and discussion on experiences of working with the DeStress project, and how knowledge coproduction was influenced by an engaged research approach.ResultsAn engaged research approach influenced the process and delivery of the DeStress project, creating a space where community partners felt empowered to coproduce knowledge relating to poverty‐related mental distress, treatment and the training of health professionals that would otherwise have been missed. We examine motivations for involvement, factors sustaining engagement, how coproduction influenced research analysis, findings and dissemination of outputs, and what involvement meant for different stakeholders.ConclusionEngaged research supported the coproduction of knowledge in mental health research with low‐income communities which led to multiple impacts.
A smart nanofibre sensor based on anthocyanin/poly‐l‐lactic acid for mutton freshness monitoring
Authors: Sun Wuliang , Liu Yilin , Jia Lu , Saldaña Marleny D. A. , Dong Tungalag , Jin Ye , Sun Wenxiu 
Published: May 2020

SummaryFood poisoning is a major issue worldwide. However, methods for identifying contaminants in foods are inefficient. Here, a solid dye was designed for mutton freshness monitoring by incorporating blueberry‐derived anthocyanins and degradable poly‐l‐lactic acid (PLLA). The solid dye was easily processed into ink, soft transparent film and nanofibre film. The nanofibre film prepared by electrostatic spinning (sensor) showed good colour stability at 4–25 °C. As the ammonia concentration increased, the sensor colour changed from pink to light pink and then to colourless. The detection limit was 37 ppm. The sensor could be repeatedly used. Applying the sensor to the detection of mutton freshness, it was found that the sensor colour change was directly related to the quality of mutton. The sensor could effectively monitor mutton freshness in real time and the colour changes were easily distinguished by naked eyes, suggesting its potential in intelligent packaging for freshness monitoring of meat foods.
Medical emergency team training: needs assessment, feedback and learning objectives
Authors: Le Guen Maurice , Costa‐Pinto Rahul 
Published: May 2020

AbstractBackgroundThere are no published studies assessing learning needs and attitudes prior to attending a medical emergency team (MET) education programme.AimsTo conduct a learning needs assessment of MET education programme participants to assess what technical and non‐technical skills should be incorporated.MethodsAll participants in a MET education programme over a 12‐month period were invited to complete a self‐administered electronic survey. Participants were ICU team members (intensive care registrars and nurses) and medical registrars. Responses were captured through a 5‐point Likert scale.ResultsThere were 62 responses out of 112 participants (55% response rate). Most participants either agreed or strongly agreed that MET training was valuable (59 respondents) and should be multidisciplinary (61 respondents). ICU team members were more likely to select ‘Management of End‐of‐Life Care’ (72% compared with only 16% of medical registrars, P < 0.05) as an important learning objective. Non‐technical skills such as ‘Task Management’ (67% compared with 37%, P < 0.05) and ‘Team Communication’ (79% compared with 32%, P < 0.05) were also more likely to be selected by ICU team members. Nursing team members were more likely to select ‘Approach to Common MET Calls’ (100% compared with 50% of medical team members, P < 0.05).ConclusionsMET education programme participants overwhelmingly feel that training should be multidisciplinary. However, there are disparities between the perceived learning needs of medical and nursing personnel, and between intensive care team members and medical registrars, which may impact on the design and implementation of a multidisciplinary education programme.
Large third‐order optical nonlinearity of chalcogenide glasses within gallium‐tin‐selenium ternary system
Authors: Chen Feifei , Zhang Jiao , Cassagne Christophe , Boudebs Georges 
Published: May 2020

AbstractWe explore the third‐order optical nonlinear properties of arsenic‐ and germanium‐free chalcogenide glasses (ChGs) within a gallium‐tin‐selenium (Ga‐Sn‐Se, GSS) ternary system at wavelength of 1.064 μm in the picosecond region. A classical characterization showing the values of the absorption and the linear index will be given. Then, using D4σ‐Z‐scan technique, both nonlinear refractive index (n2) and nonlinear absorption coefficient (β) of the GSS ChGs will be measured. A comparison of the results is made with other families of chalcogenide glasses considering the figure of merit calculation. The first results show that GSS system would give better NL performance promising high potential for n2(β)‐based photonic devices performed under clean production and green/sustainable chemistry.
Willingness of Chinese nurses to practice in Hubei combating the coronavirus disease 2019 epidemic: A cross‐sectional study
Authors: Gan Xiaoqing , Shi Zeya , Chair Sek Ying , Cao Xi , Wang Qun 
Published: May 2020

AbstractAimsTo investigate the willingness of Chinese nurses to practice in Hubei combating the coronavirus disease 2019 and to explore the associated factors.DesignA cross‐sectional survey.MethodsClinical nurses were conveniently recruited by an online link in three provinces out of Hubei, including Hunan (Central south), Chongqing (Southwest) and Xinjiang (Northwest) during 4–10 February 2020. A structured questionnaire was distributed by an online investigation system. Information on sociodemographic characteristics, willingness, possible influencing factors (previous experience, health status, training conditions, perceptions on volunteering to practice in Hubei, family attitude, and insurance) was collected. Binary logistic regression was conducted to explore the association of different factors with the willingness decision of nurses.ResultsA total of 11,183 nurses participated in this survey and a high proportion of them were willing to volunteer to practice in Hubei combating the epidemic. Nurses who were likely to volunteer had the following characteristics: younger, unmarried, members of the Communist Party of China, with senior professional qualification, working in critical care departments, with support from their families, with adequate training and learning, with good health status and low levels of anxiety. The regression model could explain 31.1% of the variances of the willingness decision of nurses.ConclusionsA high proportion of nurses in China were willing to practice in Hubei during the coronavirus disease 2019 epidemic. Adequate training and psychological support would facilitate nurses to volunteer during the outbreak of an infectious disease.ImpactThe study identified a high proportion of nurses in China were willing to practice in Hubei combating the coronavirus disease 2019 epidemic. The findings will provide valuable references for nurses and decision makers to formulate better plans for increasing nursing workforce during such kind of public health crisis.
A novel lncRNA BADLNCR1 inhibits bovine adipogenesis by repressing GLRX5 expression
Authors: Cai Hanfang , Li Mingxun , Jian Wang , Song Chengchuang , Huang Yongzhen , Lan Xianyong , Lei Chuzhao , Chen Hong 
Published: May 2020

AbstractAdipogenesis is a complex cellular process, which needs a series of molecular events, including long non‐coding RNA (lncRNA). In the present study, a novel lncRNA named BADLNCR1 was identified as a regulator during bovine adipocyte differentiation, which plays an inhibitory role in lipid droplet formation and adipogenic marker gene expression. CHIPR‐seq data demonstrated a potential competitive binding motif between BADLNCR1 and sterol regulatory element‐binding proteins 1 and 2 (SREBP1/2). Dual‐luciferase reporter assay indicated target relationship between KLF2 and BADLNCR1. Moreover, after the induction of KLF2, the expression of adipogenic gene reduced, while the expression of BADLNCR1 increased. Real‐time quantitative PCR (qPCR) showed that BADLNCR1 negatively regulated mRNA expression of GLRX5 gene, a stimulator of genes that promoted formation of lipid droplets and expression of adipogenic genes. GLRX5 could partially reverse the effect of BADLNCR1 in bovine adipocyte differentiation. Dual‐luciferase reporter assay stated that BADLNCR1 significantly reduced the enhancement of C/EBPα on promoter activity of GLRX5 gene. Furthermore, CHIP‐PCR and CHIRP‐PCR confirmed the suppressing effect of BADLNCR1 on binding of C/EBPα to GLRX5 promoter. Collectively, this study revealed the molecular mechanisms underlying the negative regulation of BADLNCR1 in bovine adipogenic differentiation.
Prognostic values of YTHDF1 regulated negatively by mir‐3436 in Glioma
Authors: Xu Chenyang , Yuan Bingjian , He Tao , Ding Bingqian , Li Song 
Published: May 2020

AbstractM6A methylation is likely to be closely associated with the occurrence and development of tumours. In this study, we demonstrated that the transcription levels of the m6A RNA methylation regulators are closely related to the prognosis of glioma. Univariate Cox analysis was performed on the expression levels of methylation regulators and selected three hub genes in glioma. Next, we systematically compared the expression of these m6A RNA methylation regulators in gliomas with different clinicopathological features. The overall survival (OS) curve of the hub genes was initially established based on TCGA database information. YTHDF1 was selected from the hub genes following survival and prognosis analysis. A nomogram was developed to predict the survival probability. We further performed cell function and in vivo xenograft tumour experiments to further verify its role in tumour progression. Next, based on the miRanda and miRDB databases, we predicted one microRNA, hsa‐mir‐346, that might regulate and bind to 3'UTR of YTHDF1, which was confirmed by our fluorescent enzyme reporter gene experiment. In summary, m6A RNA methylation regulators play a potential role in the progression of gliomas. YTHDF1 may have an essential function in glioma diagnosis, treatment and prognosis.
Tumour‐derived exosomal miR‐3473b promotes lung tumour cell intrapulmonary colonization by activating the nuclear factor‐κB of local fibroblasts
Authors: Du Cancan , Duan Xixi , Yao Xiaohan , Wan Jiajia , Cheng Yanru , Wang Yuan , Yan Yan , Zhang Lijing , Zhu Linyu , Ni Chen , Wang Ming , Qin Zhihai 
Published: May 2020

AbstractTumour‐derived exosomes have been shown to induce pre‐metastatic niche formation, favoring metastatic colonization of tumour cells, but the underlying molecular mechanism is still not fully understood. In this study, we showed that exosomes derived from the LLC cells could indeed significantly enhance their intrapulmonary colonization. Circulating LLC‐derived exosomes were mainly engulfed by lung fibroblasts and led to the NF‐κB signalling activation. Further studies indicated that the exosomal miR‐3473b was responsible for that by hindering the NFKB inhibitor delta's (NFKBID) function. Blocking miR‐3473b could reverse the exosome‐mediated NF‐κB activation of fibroblasts and decrease intrapulmonary colonization of lung tumour cells. Together, this study demonstrated that the miR‐3473b in exosomes could mediate the interaction of lung tumour cells and local fibroblasts in metastatic sites and, therefore, enhance the metastasis of lung tumour cells.
FOXO1 contributes to diabetic cardiomyopathy via inducing imbalanced oxidative metabolism in type 1 diabetes
Authors: Yan Dan , Cai Yin , Luo Jierong , Liu Jingjin , Li Xia , Ying Fan , Xie Xiang , Xu Aimin , Ma Xiaosong , Xia Zhengyuan 
Published: May 2020

AbstractForkhead box protein O1 (FOXO1), a nuclear transcription factor, is preferably activated in the myocardium of diabetic mice. However, its role and mechanism in the development of diabetic cardiomyopathy in non‐obese insulin‐deficient diabetes are unclear. We hypothesized that cardiac FOXO1 over‐activation was attributable to the imbalanced myocardial oxidative metabolism and mitochondrial and cardiac dysfunction in type 1 diabetes. FOXO1‐selective inhibitor AS1842856 was administered to streptozotocin‐induced diabetic (D) rats, and cardiac functions, mitochondrial enzymes PDK4 and CPT1 and mitochondrial function were assessed. Primary cardiomyocytes isolated from non‐diabetic control (C) and D rats were treated with or without 1 µM AS1842856 and underwent Seahorse experiment to determine the effects of glucose, palmitate and pyruvate on cardiomyocyte bioenergetics. The results showed diabetic hearts displayed elevated FOXO1 nuclear translocation, concomitant with cardiac and mitochondrial dysfunction (manifested as elevated mtROS level and reduced mitochondrial membrane potential) and increased cell apoptosis (all P < .05, D vs C). Diabetic myocardium showed impaired glycolysis, glucose oxidation and elevated fatty acid oxidation and enhanced PDK4 and CPT1 expression. AS1842856 attenuated or prevented all these changes except for glycolysis. We concluded that FOXO1 activation, through stimulating PDK4 and CPT1, shifts substrate selection from glucose to fatty acid and causes mitochondrial and cardiac dysfunction.
Characterization of the monoclonal antibody specific to the ORF72 protein of koi herpesvirus and cellular distribution analysis of the viral protein
Authors: Wu Ching‐Wei , Wu Ting‐Yun , Kuo Chih‐Jung , Lu Yi‐Ping , Chien Maw‐Sheng , Huang Chienjin 
Published: May 2020

AbstractKoi herpesvirus (KHV) is an emerging pathogen of koi and common carp that causes a severe disease and mass mortality of infected fish. The KHV ORF72 protein is an important capsid protein that has been suggested to be a candidate for the development of diagnostic reagents and KHV vaccines. The purpose of this study was to clone and express the KHV ORF72 gene for further preparation of a specific monoclonal antibody (mAb) and to analyse cellular distribution of the viral protein. The mAb 3E1 could specifically recognize the expressed ORF72 protein of transfected cells by indirect immunofluorescence, and the antigenic site recognized by the mAb 3E1 was mapped to the region of N‐terminal 124 residues of KHV ORF72. This mAb was further demonstrated to specifically detect the KHV‐infected fish tissue by immunohistochemistry, thereby suggesting its high diagnostic potential. In addition, the cellular distribution analysis of the KHV ORF72 protein revealed that the region of amino acid residues 125–247 was related to mitochondrial localization and proliferation. Furthermore, a putative nuclear export signal (NES) of ORF72 at the residues 201–212 was confirmed on the basis of its function associated with NES activity.
The carbon footprint of foreign multinationals within the European Union
Authors: Ortiz Mateo , Cadarso María‐Ángeles , López Luis‐Antonio 
Published: May 2020

AbstractAlthough European countries have made great efforts to reduce their territorial carbon emissions, global emissions are still growing. Multinational enterprises (MNEs) operating within Europe, as transnational institutions, can make significant contributions in translating European efforts into global emissions reduction. Here, we estimate the carbon footprint of the foreign multinationals’ affiliates (FMNEs) operating within the European Union (EU) in 2015 as a first assessment of the MNEs’ potential regarding European and global carbon emissions reduction targets. Our findings show that FMNEs generate 17% of the total carbon footprint of the EU but only 12% of the total value added. Thus, the net impacts of FMNE are considered to be in environmental deficit because their adverse environmental impacts are relatively higher than their positive economic ones. Calculations are made under the assumption that FMNEs produce using the same technology as their domestic peers; therefore, the carbon/economic imbalance found is attributed to the FMNEs’ distribution across sectors. The participation of FMNEs in carbon‐intensive industrial sectors are remarkably high in low‐income EU members; therefore, the effective reduction of the carbon footprint in those countries is largely conditioned by the decisions of foreign MNEs’ headquarters. Furthermore, those countries are more vulnerable to capital leakages in the case where a European carbon tax was to be imposed. We conclude by discussing the economic and policy implications of the country‐level inequality of MNEs’ environmental impacts within the EU.
Co‐regulation of indole glucosinolates and camalexin biosynthesis by CPK5/CPK6 and MPK3/MPK6 signaling pathways
Authors: Yang Liuyi , Zhang Yan , Guan Rongxia , Li Sen , Xu Xuwen , Zhang Shuqun , Xu Juan 
Published: May 2020

AbstractSecondary plant metabolites, represented by indole glucosinolates (IGS) and camalexin, play important roles in Arabidopsis immunity. Previously, we demonstrated the importance of MPK3 and MPK6, two closely related MAPKs, in regulating Botrytis cinerea (Bc)‐induced IGS and camalexin biosynthesis. Here we report that CPK5 and CPK6, two redundant calcium‐dependent protein kinases (CPKs), are also involved in regulating the biosynthesis of these secondary metabolites. The loss‐of‐function of both CPK5 and CPK6 compromises plant resistance to Bc. Expression profiling of CPK5‐VK transgenic plants, in which a truncated constitutively active CPK5 is driven by a steroid‐inducible promoter, revealed that biosynthetic genes of both IGS and camalexin pathways are coordinately upregulated after the induction of CPK5‐VK, leading to high‐level accumulation of camalexin and 4‐methoxyindole‐3‐yl‐methylglucosinolate (4MI3G). Induction of camalexin and 4MI3G, as well as the genes in their biosynthesis pathways, is greatly compromised in cpk5 cpk6 mutant in response to Bc. In a conditional cpk5 cpk6 mpk3 mpk6 quadruple mutant, Bc resistance and induction of IGS and camalexin are further reduced in comparison to either cpk5 cpk6 or conditional mpk3 mpk6 double mutant, suggesting that both CPK5/CPK6 and MPK3/MPK6 signaling pathways contribute to promote the biosynthesis of 4MI3G and camalexin in defense against Bc.
Challenges, Extent of Involvement, and the Impact of Nurses’ Involvement in Politics and Policy Making in in Last Two Decades: An Integrative Review
Authors: Rasheed Subia Parveen , Younas Ahtisham , Mehdi Fahmida 
Published: May 2020

AbstractPurposeTo determine nurses' challenges, extent of involvement, and the impact of involvement in politics and policy making.Organizing Construct: Nurses in politics and health policy making.MethodsLiterature was searched in PubMed, Scopus, Google Scholar, the Cumulative Index to Nursing and Allied Health Literature (CINAHL), OVID, and Open Grey using phrases comprising the following key words: “nurses”, “policy making”, “politics”, “health policy”, “nurses involvement in policy making/politics/health policy”, “nurses challenges in policy making/politics/policy”, and “impact of nursing policy making/politics/health policy”; 22 articles published from January 2000 to May 2019 were included.FindingsThe major challenges included intra‐ and interprofessional power dynamics, marginalization of nurses in policy making, and nursing profession–specific challenges. The extent of involvement was inadequate, and nurses mainly worked as policy implementers rather than as policy developers. Those nurses who participated in policy development focused on health promotion to build healthy communities and to empower nurses and the nursing profession.ConclusionsNurses' involvement in policy making has not improved over time. Nursing institutions and regulatory bodies should prepare and encourage nurses to work as policymakers rather than implementers and advocate for the rightful place of nurses at policy‐making forums.Clinical RelevancePreparation for health system policy making starts in the clinical settings. Educational institutions and nurse leaders should adequately prepare nurses for policy making, and nurses should participate in policy making at the organization, system, and national levels.
Cerebral Blood Flow Velocity is Not Associated with Serum Hemoglobin in Children with Malaria‐Associated Anemia
Authors: Taty Tshimanga M. , Mabiala Joseph B. , Lovett Marlina E. , Pongo Jean , Musungufu Davin A. , Uchama Mananu , O'Brien Nicole F. 
Published: May 2020

ABSTRACTBACKGROUND AND PURPOSEHemoglobin (Hbg) is often thought to impact cerebral blood flow velocity (CBFV). This study was performed to investigate the relationship between Hbg value and CBFV in African children with malaria.METHODSIn this prospective, observational study, children aged 3 months to 18 years with malaria and a normal Blantyre coma score underwent a single transcranial Doppler ultrasound (TCD) examination with a concurrent Hbg check.RESULTSOne hundred fifty‐six children with a mean age of 43 months were enrolled. Thirty‐three children (21%) had severe anemia (Hbg <5g/dL), 46 (29%) had moderate anemia (Hbg 5‐6.9 g/dL), 63 children (41%) had mild anemia (7‐9.9 g/dL), and 14 children (9%) had no anemia (Hbg >10 g/dL) at the time of TCD examination. Mean averaged CBFV in the middle cerebral artery (MCA) for the cohort was 99% of predicted based on normative values standardized for age. There was no significant correlation between Hbg levels and measured CBFV in the MCA (r = −.09; 95% CI, −.24‐.07; P = .29).CONCLUSIONIn a large sample of African children with malaria, Hbg did not correlate with CBFVs as measured by TCD. Future work that includes baseline TCD measurements and Hbg values as well as other physiological parameters known to influence CBFVs is necessary to confirm these findings.