bluuebunny/crossref_metadata_2025_split huggingface dataset:
Rheumatologists' understanding of Refractory Gout: A questionnaire survey in China
Authors: Han Xinxin , Yin Yue , Cao Yu , Chu Xiaotian , Han Yingdong , Di Hong , Xu Na , Shen Min , Zeng Xuejun , Zhang Yun 
Published: March 2020

Abstract
Background Refractory gout is a common problem faced by experienced rheumatologists, relevant research on the difficulties of gout treatment and the understanding of "refractory gout" by rheumatologists is lacking. Methods A total of 910 Chinese rheumatologists attending the annual academic conference on rheumatism completed the electronic questionnaires. The differences in the judgment of refractory gout were analyzed between the senior experience (SE) group and the general experience (GE) group. Results A total of 751 (82.5%) respondents with relevant continuing medical education (CME) were included in our study, including 175 (23.3%) SE rheumatologists and 576 (76.7%) GE rheumatologists. Most of the rheumatologists (715, 95.2%) considered renal function insufficiency as a common complication making gout challenging to treat, while rheumatologists with different experiences had different attitudes towards obesity. For the chronic gouty arthritis patients with one or more tophi, 476 (63.9%) Chinese rheumatologists preferred febuxostat as the first-line treatment option, while 582 (78.3%) chose the maximum daily dose of 80mg for febuxostat, with a higher proportion in SE group than GE group. Meanwhile, the proportion of doctors who chose the dosage of all three uric-lowering drugs consistently with the guidelines was higher in the SE group. Moreover, 123 (70.3%) people in the SE group thought that regardless of the use of drugs for prevention of attack during ULT, the recurrence of symptoms was symptomatic of refractory gout, while fewer in GE group agreed. Similarly, 119 (68.0%) in the SE group thought that failure to achieve the targeted serum uric acid level within 6 months of standardized urate-lowering therapy could be assigned to refractory gout, while fewer in GE group agreed. In terms of personal and social factors, the SE group had a higher percentage of physicians concerned about poor compliance leading to irregular treatment and drug abuse. Conclusions Most of the rheumatologists selected uric-lowering drugs and the maximum dosage of medicines consistently with guidelines. Uncontrolled symptoms, unattainable therapeutic targets, and unimproved lifestyle and compliance of patients are difficulties that Chinese rheumatologists have to face with during gout treatment.
Deformity progression in congenital posteromedial bowing of the tibia: a report of 44 cases.
Authors: Gennaro Giovanni Luigi Di , Gallone Giovanni , Vazquez Edgar Alejandro Martinez , Reggiani Leonardo Marchesini , Racano Costantina , Olivotto Eleonora , Stilli Stefano , Trisolino Giovanni 
Published: March 2020

Abstract
Background Congenital posteromedial bowing of the tibia (CPMBT) is an ultra-rare defect present at birth, characterized by shortened bowed leg, and ankle deformity. We described a single institution experience in the management of CPMBT Methods We identified 44 CPMBT in 44 children. The age at presentation was 5.5 ± 5.6 years and the mean age at the final review was 10.1 ± 4.8 years. Radiographic evaluation included the antero-posterior and lateral inter-physeal angle (AP-IPA and L-IPA), the limb length discrepancy (LLD), the morphology of the distal tibia and the lateral distal tibial angle (LDTA). During the study period, 26 children underwent surgical treatment. Results The estimated curves showed a progressive spontaneous correction of both AP-IPA and L-IPA during growth, but a progressive increase of the LLD. The L-IPA showed a more predictable behaviour while the AP-IPA showed a scattered correction, with a wider variation of the estimated final angle. The final LDTA was 83.4° ± 5.2° and was correlated with the L-IPA. Among the 26 children which underwent surgical treatment, 23 cases had limb lengthening, 1 case had contralateral epiphysiodesis, 1 child underwent tibial osteotomy, 1 patient was treated by hemiepiphysiodesis of the distal tibia to address ankle valgus deformity. Conclusions To date, we reported the largest case series of CPMBT. Nevertheless, further studies are needed to understand which is the best strategy to address this ultra-rare deformity during childhood.
Ongoing Donor-transmitted Diabetic Kidney Disease in Kidney Transplant Recipients with Fair Sugar Control: a single center retrospective study
Authors: Hsu Chia-Tien , Wen Mei-Chin , Chiu Hsien-Fu , Tsai Shang-Feng , Yu Tung-Min , Yang Cheng-Kuang , Wu Ming-Ju , Chen Cheng-Hsu 
Published: March 2020

Abstract
Background Transplantation with a diabetic donor kidney may have some benefits compared to remaining on the waitlist for selected patients. However, we found that some kidney transplant recipients have ongoing donor-transmitted diabetic kidney disease (DT-DKD) despite fair blood sugar control. This study aimed to survey the incidence and clinical pattern of DT-DKD in kidney transplant recipients.Methods We retrospectively reviewed the medical records of kidney transplantations in our hospital. We found 357 kidney transplantations from February 2006 to April 2018. Among these, 23 (6.4%) diabetic donor kidney transplantations were done in the study period.Results Among the 23 recipients, 6 (26.1%) displayed biopsy-proven DKD. Recipients with biopsy-proven DKD had longer dialysis vintage, higher proteinuria amount, lower last estimated glomerular filtration rate (eGFR), and a more rapid decline in the eGFR. The median fasting blood sugar level in the biopsy-proven DKD group was unexpectedly lower than the non-DKD group. Most of the pre-implantation frozen sections in biopsy-proven DKD group showed diabetic lesions worse than diabetic nephropathy (DN) class IIa. In the biopsy-proven DKD group, 5 recipients had no history of diabetes before or after transplantation. Among the 23 recipients, 5 (21.7%) were diagnosed with DT-DKD. Serial post-transplant biopsies showed the histological progression of allograft DN.Conclusions To the best of our knowledge, this is the first study to report the phenomenon of ongoing DT-DKD in kidney transplant recipients with fair blood sugar control. The zero-time pre-transplant kidney biopsy may be an important examination before the allocation of diabetic donor kidneys.
First comprehensive proteomics analysis of lysine crotonylation in leaves of peanut (Arachis hypogaea L.)
Authors: Xu Manlin , Luo Jianda , Li Ying , Shen Lili , Zhang Xia , Yu Jing , Guo Zhiqing , Wu Juxiang , Chi Yucheng , Yang Jinguang 
Published: March 2020

Abstract
Background: Lysine crotonylation is an important post-translational modification (PTM) process. Most research in this area has been carried out on mammals and yeast, but there has been no research published on crotonylated proteins on peanut.Results: In the current study, large-scale lysine crotonylation analysis was performed by a combination of affinity enrichment and high-resolution LC-MS/MS analysis. Altogether, 6051 lysine crotonylation sites were identified in 2508 protein groups. Bioinformatics analysis showed that lysine-crotonylated proteins were involved in many biological processes, such as carbon fixation in photosynthetic organisms, photosynthesis, biosynthesis of amino acids, ribosomes, etc. In particular, subcellular localization analysis showed that 43% of the crotonylated proteins were located in the chloroplast and that there were 29 crotonylated proteins associated with photosynthesis. In addition, 26 crotonylated proteins were identified in photosynthesis network and 145 proteins were mapped to ribosome network, indicating the diverse functions for lysine crotonylation in peanut.Conclusion: These data show that crotonylated proteins play a major part in peanut biological functions including carbon fixation in photosynthetic organisms, photosynthesis, biosynthesis of amino acids, ribosomes, etc. A lot of proteins related to photosynthesis and ribosome suggest that lysine crotonylation may play important regulatory roles in their structure and function. This dataset is the first comprehensive proteomics analysis of lysine crotonylation in peanut and will serve as an important resource with which to study the biosynthesis and function of lysine crotonylation in peanut and related plants. Based on these results, further studies to expand on the lysine crotonylation analysis were suggested. Data are available via ProteomeXchange with the identifier PXD017675.
Systematic Identification and Analysis of NAC Gene Family in Moso Bamboo (Phyllostachys edulis)
Authors: Gao Yameng , Liu Huanlong , Wu Lin , Xiong Rui , Shi Yanan , Xiang Yan 
Published: March 2020

Abstract
Background: NAC (NAM/ATAF1/2/CUC2) gene family is a large plant-specific transcription factor family, which is implicated in many functions, such as morphogenesis, the thickness formation of secondary cell walls as well as biotic and abiotic stress and more. In moso bamboo ( Phyllostachys edulis ), 94 PeNACs have been identified and three members are predicted to relate to the secondary cell wall. However, there were few studies on moso bamboo NAC genes under stress.Results: In this study, we re-identified 165 PheNACs with the latest moso bamboo genome data and divided them into 12 subfamilies using NAM domains. Gene structure and motif distribution manifested the NAC gene family was fairly conserved. Evolutionary analysis showed that the segmental duplication played a significant role in the expansion of NAC genes and the relationship between moso bamboo and Brachypodium distachyon was closest than beween moso bamboo and other four species ( Arabidopsis thaliana, Oryza sativa , Sorghum bicolor and Zea mays ). Based on the promoter analysis of the 27 NAC members in A subfamily, quantitative real-time PCR exhibited these genes reacted differently under drought, high salt, abscisic acid and methyl jasmonate treatments. Finally, we selected out four potential stress-associated genes (PheNAC001, -056, -080 and -100) and found they all localized in the tobacco nucleus and had transcriptional activity in yeast.Conclusions: These preliminary results provide valuable information for mining potential resistance NAC genes and lay theoretical basis for breeding new stress-resistant varieties in moso bamboo.
An Empirical Analysis of the Socioeconomic and Demographic characteristics influencing Birth Certification in Nigeria
Authors: Anaduaka Uchechi Shirley 
Published: March 2020

Abstract
Background: Promoting birth certification is instrumental to achieving target 16.9 of the Sustainable Development Goals: legal identity for all by 2030. Currently, the birth certification rates are very low: on average, only 16.6% of children under-five years have their births certified. Methods: Using the nationally representative Nigerian Demographic and Health Survey, this paper analyzes the socioeconomic and demographic factors associated with the birth certification of children under-five years. The relationship with these factors and birth certification were analyzed using robust econometric techniques – ordinary least squares and multilevel regression approaches.Results: The study finds that access to health services, parental education and household ‌‌wealth strongly influence birth certification. Conversely, distance to registration center, higher birth orders, longer birth intervals and father’s working status are significant obstacles to birth certification. Finally, child age, maternal age at birth and father age have non-linear effects on birth certification among children under-five years in Nigeria. I find no significant effects of gender on birth certification. Conclusions: Improving access to health services and anchoring birth certification on child-targeted conditional cash transfer programs could be significant policy instruments for increasing birth certification in Nigeria.
A Review and Synthesis of TBPCC Evaluation in Canada: Looking Beyond the Quadruple Aim
Authors: Fletcher Sarah C. , Bellwood Paule , Hill Tiffany T. , Martin Susan , Ashe Maureen C. , Price Morgan 
Published: March 2020

Abstract
Background The objectives of this article are: 1. To synthesize peer-reviewed evidence on the outcomes of team-based primary and community care (TBPCC) in Canada on Patient and Provider Experience, Population Health, and Health Care Costs (Quadruple Aim); 2. To introduce the TBPCC Evaluation Framework; and 3. To extend the critical interpretive synthesis to include the additional four domains from the TBPCC Evaluation Framework (i.e., Relationship Centred Care, Care Process and Quality, Team Function and TBC Foundations). Methods We conducted a review of the following databases: Medline (OVID and PubMed), CINAHL, Embase, SportDiscus, and PsycINFO as well as an advanced search with Google Scholar (Title only) with the words “Canada AND primary AND team”. Review concepts included: population (patients), intervention (team-based primary health care), comparator (usual care, single practitioner delivery mode), outcome (patient and provider experience, population health, and health care costs), time (2000-present), and type (randomized controlled trials, controlled trials, quasi-experimental designs, and implementation studies/evaluations). We excluded reviews, opinion papers, laboratory-based studies, and studies based outside of Canada. Results Forty-five publications met our inclusion criteria with the majority of these (34) from Central Canada. Results were initially mapped to the domains of the Quadruple Aim, with 51% (23/45) aligning. The additional domains from the TBPCC Evaluation Framework (Team Function, Relationship Centred Care, Care Process and Quality and Team-Based Care Foundations) and the Team-Based Care (TBC) Adoption Model were integrated into the synthesis. 100% of the included articles reported outcomes that aligned with the TBPCC Evaluation Framework. Conclusion Across Canada, the value of relationships, shared understanding, communication, and coordination across teams are highlighted as is the potential of TBC to result in improvements in patient and provider experience, team function, and the quality of care. By encouraging a focus on formative as well as summative evaluation, the TBPCC Evaluation Framework provides a comprehensive approach to assessing the evidence needed to support actionable improvements for TBPCC in Canada. Trial Registration To identify peer-reviewed literature, we followed standard review methodology and reporting guidelines as established by PRISMA. We registered our review on PROSPERO (2018 CRD42018091086).
Older persons are more frail after an emergency care visit to the out-of-hours General Practitioner Cooperative in the Netherlands: a cross sectional descriptive TOPICS-MDS study
Authors: Bloemhoff Anneke , Schoon Yvonne , Smulders Kien , Akkermans Reinier , Vloet Lilian , Berg Karen van den , Berben Sivera 
Published: March 2020

Abstract
Background In the Netherlands community dwelling older people with primary care emergency problems contact the General Practitioner Cooperative (GPC) after hours. However, frailty remains often unobserved with the hazard of adverse health outcomes. The aim of this study was to provide insight in differences between older persons with or without GPC emergency care visits (reference group) regarding frailty and healthcare use.Methods A cross-sectional descriptive study design was based on data from the public data repository: The Older Persons and Informal Caregivers Survey Minimum Dataset (TOPICS-MDS). Frailty in older persons (65+ years, n=32,149) was measured by co-morbidity, functional and psychosocial aspects, quality of life and a frailty index. Furthermore, home care use and hospital admissions of older persons were identified. We performed multilevel logistic and linear regression analysis. A random intercept model was utilized to test differences between groups, and adjustment factors (confounders) were used in the multilevel analysis. Results Older persons with GPC contact were, compared to the reference group, more frail on the domain of co-morbidity (mean difference 0.52 ; 95% CI 0.47-0.57, p <0.0001), functional limitations (mean difference 0.53 ; 95% CI 0.46-0.60, p <0.0001), reported less emotional wellbeing (mean difference -4.10 ; 95% CI -4.59- -3.60, p <0.0001) and experienced less quality of life (mean difference -0.057 ; 95% CI -0.064- -0.050, p <0.0001). Moreover, older persons reported more often limited social function (OR 1.50, 95%CI 1.39-1.62, p <0.0001) and limited perceived health (OR 1.50, 95%CI 1.39-1.62, p <0.0001). Finally, older persons with GPC contact more often used home care (OR 1.37, 95%CI 1.28-1.47, p <0.0001) or were more often admitted to the hospital (OR 2.88, 95%CI 2.71-3.06, p <0.0001). Conclusions Older persons with out-of-hours GPC contact for an emergency care visit were significantly more frail on all domains, more likely to use home care or to be admitted to the hospital, compared to the reference group. Potentially frail older persons seem to require adequate identification of frailty, and support (e.g. advance care planning) before and after a contact with the out-of-hours GPC.
A broad-spectrum virus- and host-targeting antiviral peptide against SARS-CoV-2 and other respiratory viruses
Authors: Zhao Hanjun , To Kelvin K. W. , Sze Kong-Hung , Yung Timothy Tin-Mong , Bian Mingjie , Lam Hoiyan , Li Cun , Chu Hin , Yuen Kwok-Yung 
Published: March 2020

Abstract
The 2019 novel respiratory virus (SARS-CoV-2) causes COVID-19 with rapid global socioeconomic disruptions and disease burden to healthcare. The current COVID-19 and previous emerging virus outbreaks highlight the urgent need for broad-spectrum antivirals. Here, we showed that a defensin-like peptide P9R exhibited potent antiviral activity against pH-dependent viruses that require endosomal acidification for virus-host membrane fusion, including the enveloped coronaviruses (SARS-CoV-2, SARS-CoV and MERS-CoV), the pandemic A(H1N1)pdm09 virus, avian influenza A(H7N9) virus, and the non-enveloped rhinovirus. P9R could significantly protect mice from lethal challenge by A(H1N1)pdm09 virus and showed low possibility to cause drug-resistance virus. Mechanistic studies indicated that the antiviral activity of P9R depended on the direct binding to viruses and the inhibition of virus-host endosomal acidification, which provides a new concept that virus-binding alkaline peptides could broadly inhibit pH-dependent viruses. These results suggest that the dual-functional virus- and host-targeting P9R could be a promising candidate for combating pH-dependent respiratory viruses.Authors Hanjun Zhao, Kelvin K. W. To, and Kong-Hung Sze contributed equally to this work.
Fresh versus frozen single embryo transfer in Chinese women of advanced age undergoing IVF/ICSI: a study protocol for a randomized controlled trial
Authors: Chen Miaoxin , Wu Yuanyuan , Huang Xin , Liu Wenqiang , Li Wentao , Sun Chunyan , Meng Zhenzhen , Mol Ben W , Teng Xiaoming 
Published: March 2020

Abstract
Background: Elective frozen cleavage embryo transfer resulted in significantly higher live birth rate in patients with polycystic ovary syndrome but not in ovulatory women compared with fresh embryo transfer. Further, elective single frozen blastocyst transfer had significantly higher live birth rate in ovulatory women compared with single fresh blastocyst transfer. However, it is unknown whether single frozen cleavage embryo transfer results in higher cumulative live birth rate in women of advanced age undergoing in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI). The aim of this study is to compare the effectiveness of frozen cleavage embryo transfer with fresh cleavage embryo transfer in Chinese women of advanced age. Methods: This study is a double-blind randomized controlled clinical trial (1:1 treatment ratio of frozen embryo transfer vs. fresh embryo transfer). A total of 840 women of advanced age with normal ovarian reserve undergoing the first cycle of IVF or ICSI will be enrolled and randomized into two parallel groups. Participants in group A will undergo frozen single cleavage embryo transfer, and participants in group B will undergo fresh single cleavage embryo transfer. The primary outcome is the cumulative live birth rate of the trial IVF/ICSI cycle within 12 months after randomization. This study is powered to detect an absolute difference of 8% (23% vs 15%) at the significance level of 0.05 and 80% statistical power based on a two-sided test. Discussion: The results of this study will provide evidence for the efficacy and safety of frozen cleavage embryo transfer compared with fresh cleavage embryo transfer in women of advanced age undergoing IVF/ICSI. Trial registration: Chinese Clinical Trial Registry, ChiCTR2000029330. Registered on 25 Jan 2020. Keywords: Frozen embryo transfer, advanced age, in vitro fertilization (IVF), intracytoplasmic sperm injection (ICSI), live birth, randomized controlled trial.
Formulation Development of Tamoxifen Loaded Lipid Nanoparticle by Taguchi (L12 (211)) Orthogonal Array Design
Authors: Poovi Ganesan , Damodharan Narayanasamy 
Published: March 2020

Background:
A better understanding of the biopharmaceutical and physicochemical
properties of drugs and the pharmaco-technical factors would be of great help for developing pharmaceutical
products. But, it is extremely difficult to study the effect of each variable and interaction
among them through the conventional approach.

Methods:
To screen the most influential factors affecting the particle size (PS) of lipid nanoparticle
(LNPs) (solid lipid nanoparticle (SLN) and nanostructured lipid carrier (NLC)) for poorly watersoluble
BCS class-II drug like tamoxifen (TMX) to improve its oral bioavailability and to reduce its
toxicity to tolerable limits using Taguchi (L12 (211)) orthogonal array design by applying computer
optimization technique.

Results:
The size of all LNPs formulations prepared as per the experimental design varied between
172 nm and 3880 μm, polydispersity index between 0.033 and 1.00, encapsulation efficiency between
70.8% and 75.7%, and drug loading between 5.84% and 9.68%. The study showed spherical
and non-spherical as well as aggregated and non-aggregated LNPs. Besides, it showed no interaction
and amorphous form of the drug in LNPs formulation. The Blank NLCs exhibited no cytotoxicity
on MCF-7 cells as compared to TMX solution, SLNs (F5) and NLCs (F12) suggest that the
cause of cell death is primarily from the effect of TMX present in NLCs.

Conclusions:
The screening study clearly showed the importance of different individual factors
significant effect for the LNPs formulation development and its overall performance in an in-vitro
study with minimum experimentation thus saving considerable time, efforts, and resources for further
in-depth study.