bluuebunny/crossref_metadata_2025_split huggingface dataset:
A novel modelling approach to quantify the response of dairy goats to a high-concentrate diet
Authors: Taghipoor Masoomeh , Delattre Maud , Giger-Reverdin Sylvie 
Published: November 2020

AbstractHigh-producing ruminants need high-concentrate diets to satisfy their nutrient requirements and meet performance objectives. However, such diets induce sub-acute ruminal acidosis (SARA), which will adversely affect dry matter intake and lead to lower production performance. This work develops a novel modelling approach to quantify the capacity of dairy goats to adapt to a high-concentrate diet challenge at the individual level. The animal model used was dairy goats (from Saanen or Alpine breed), and rumen pH was used as the indicator of the response. A three-step modelling procedure was developed to quantify daily scores and produce a single global index for animals’ adaptive response to the new diet. The first step summarizes the post-prandial kinetics of rumen acid status using three synthetic variables. In the second step, the effect of time on the response of goats is described, in the short and long terms. In the last step, a metric based on phase trajectories ranks goats for their resilience capacity. This modelling procedure showed a high variability among the goats in response to the new diet, highlighting in particular their daily and general strategies to buffer the effect of the diet change. Two main categories of adaptive strategies were observed: (i) acid status increased, but the goats tried to minimize its variations, and (ii) acid status oscillated between increases and decreases. Such phenotyping, alongside other behavioral, digestive, and metabolic measures, can help to determine biomarkers of goats’ capacity to adapt to diets of higher nutritive value and to increase production performance without compromising their health status. Quantifying the capacity of goats to buffer the effect of highly fermentable diets helps to better adapt feed to animals in precision livestock farming. This procedure is generic and can be adapted to any indicator of animal health and performance. In particular, several indicators can be combined to assess multi-performance, which is of major interest in the context of selection for robust animals.
Effects of inflammatory and anti-inflammatory environments on the macrophage mitochondrial function
Authors: Ji Dong , Yin Jian-yun , Li Dan-feng , Zhu Chang-tai , Ye Jian-ping , Pan Yuan-qing 
Published: November 2020

AbstractMitochondrial response to inflammation is crucial in the metabolic adaptation to infection. This study aimed to explore the mitochondrial response under inflammatory and anti-inflammatory environments, with a focus on the tricarboxylic acid (TCA) cycle. Expression levels of key TCA cycle enzymes and the autophagy-related protein light chain 3b (LC3b) were determined in raw 264.7 cells treated with lipopolysaccharide (LPS) and metformin (Met). Additionally, reactive oxygen species (ROS) levels and mitochondrial membrane potential were assessed using flow cytometry. Moreover, 8-week-old C57BL/6J mice were intraperitoneally injected with LPS and Met to assess the mitochondrial response in vivo. Upon LPS stimulation, the expression of key TCA enzymes, including citrate synthase, α-ketoglutarate dehydrogenase, and isocitrate dehydrogenase 2, and the mitochondrial membrane potential decreased, whereas the levels of LC3b and ROS increased. However, treatment with Met inhibited the reduction of LPS-induced enzyme levels as well as the elevation of LC3b and ROS levels. In conclusion, the mitochondrial TCA cycle is affected by the inflammatory environment, and the LPS-induced effects can be reversed by Met treatment.
Impact of deranged B cell subsets distribution in the development of HCV-related cirrhosis and HCC in type two diabetes mellitus
Authors: Abdelwahab Fadwa A. , Hassanein Khaled M. , Hetta Helal F. , Abdelmalek Mohamed O. , Zahran Asmaa M. , El-Badawy Omnia 
Published: November 2020

AbstractType II diabetes (T2D) may worsen the course of hepatitis C virus infection with a greater risk of liver cirrhosis (LC) and hepatocellular carcinoma (HCC). In chronic viral infections, the deranged B cell subset signifies uncontrolled disease. The study aimed to verify the relation between B cell subsets’ distribution and liver disease progression in chronic hepatitis C (CHC) patients with T2D. A total of 67 CHC patients were divided into two groups; 33 non-diabetic and 34 with T2D. Each group was subdivided into CHC-without LC or HCC (N-CHC), CHC-with LC (CHC-LC), and CHC-with HCC (CHC-HCC). Twenty-seven healthy individuals also participated as controls. Flow cytometry was used to analyze CD19+ B cell subsets based on the expression of CD24 and CD38. CD19+CD24hiCD38hi Immature/transitional B cells elevated in diabetic than non-diabetic patients. In diabetic patients, while CD19+CD24+CD38− primarily memory B cells were higher in CHC-N and CHC-HCC groups than LC with a good predictive accuracy of LC, the opposite was observed for CD19+CD24−CD38− new memory B cells. Only in diabetic patients, the CD19+CD24intCD38int naïve mature B cells were high in CHC-HCC patients with good prognostic accuracy of HCC. Merely in diabetic patients, several correlations were observed between B cell subsets and liver function. Immature/transitional B cells increase remarkably in diabetic CHCpatients and might have a role in liver disease progression. Memory and Naïve B cells are good potential predictors of LC and HCCin diabetic CHCpatients, respectively. Further studies are needed to investigate the role of the CD19+CD24−CD38− new memory B cells in disease progression in CHC patients.
Exploiting the biological response of two Serratia fonticola strains to the critical metals, gallium and indium
Authors: Caldeira Joana B. , Morais Paula V. , Branco Rita 
Published: November 2020

AbstractThe use of microorganisms that allows the recovery of critical high-tech elements such as gallium (Ga) and indium (In) has been considered an excellent eco-strategy. In this perspective, it is relevant to understand the strategies of Ga and In resistant strains to cope with these critical metals. This study aimed to explore the effect of these metals on two Ga/In resistant strains and to scrutinize the biological processes behind the oxidative stress in response to exposure to these critical metals. Two strains of Serratia fonticola, A3242 and B2A1Ga1, with high resistance to Ga and In, were submitted to metal stress and their protein profiles showed an overexpressed Superoxide Dismutase (SOD) in presence of In. Results of inhibitor-protein native gel incubations identified the overexpressed enzyme as a Fe-SOD. Both strains exhibited a huge increase of oxidative stress when exposed to indium, visible by an extreme high amount of reactive oxygen species (ROS) production. The toxicity induced by indium triggered biological mechanisms of stress control namely, the decrease in reduced glutathione/total glutathione levels and an increase in the SOD activity. The effect of gallium in cells was not so boisterous, visible only by the decrease of reduced glutathione levels. Analysis of the cellular metabolic viability revealed that each strain was affected differently by the critical metals, which could be related to the distinct metal uptakes. Strain A3242 accumulated more Ga and In in comparison to strain B2A1Ga1, and showed lower metabolic activity. Understanding the biological response of the two metal resistant strains of S. fonticola to stress induced by Ga and In will tackle the current gap of information related with bacteria-critical metals interactions.
Historical predictability of rainfall erosivity: a reconstruction for monitoring extremes over Northern Italy (1500–2019)
Authors: Diodato Nazzareno , Ljungqvist Fredrik Charpentier , Bellocchi Gianni 
Published: November 2020

AbstractErosive storms constitute a major natural hazard. They are frequently a source of erosional processes impacting the natural landscape with considerable economic consequences. Understanding the aggressiveness of storms (or rainfall erosivity) is essential for the awareness of environmental hazards as well as for knowledge of how to potentially control them. Reconstructing historical changes in rainfall erosivity is challenging as it requires continuous time-series of short-term rainfall events. Here, we present the first homogeneous environmental (1500–2019 CE) record, with the annual resolution, of storm aggressiveness for the Po River region, northern Italy, which is to date also the longest such time-series of erosivity in the world. To generate the annual erosivity time-series, we developed a model consistent with a sample (for 1981–2015 CE) of detailed Revised Universal Soil Loss Erosion-based data obtained for the study region. The modelled data show a noticeable descending trend in rainfall erosivity together with a limited inter-annual variability until ~1708, followed by a slowly increasing erosivity trend. This trend has continued until the present day, along with a larger inter-annual variability, likely associated with an increased occurrence of short-term, cyclone-related, extreme rainfall events. These findings call for the need of strengthening the environmental support capacity of the Po River landscape and beyond in the face of predicted future changing erosive storm patterns.
Future changes in monsoon duration and precipitation using CMIP6
Authors: Moon Suyeon , Ha Kyung-Ja 
Published: November 2020

AbstractFuture change in summertime rainfall under a warmer climate will impact the lives of more than two-thirds of the world’s population. However, the future changes in the duration of the rainy season affected by regional characteristics are not yet entirely understood. We try to understand changes in the length of the rainy season as well as the amounts of the future summertime precipitation, and the related processes over regional monsoon domains using phase six of the Coupled Model Intercomparison Project archive. Projections reveal extensions of the rainy season over the most of monsoon domains, except over the American monsoon. Enhancing the precipitation in the future climate has various increasing rates depending on the subregional monsoon, and it is mainly affected by changes in thermodynamic factors. This study promotes awareness for the risk of unforeseen future situations by showing regional changes in precipitation according to future scenarios.
Critical charge transport networks in doped organic semiconductors
Authors: Hofacker Andreas 
Published: November 2020

AbstractIntrinsic organic small molecule and polymer materials are insulators. The discovery that polymers can be made highly conductive by doping has therefore sparked strong interest in this novel class of conductors. More recently, efficient doping of small molecule materials has also been achieved and is now a key technology in the multi-billion dollar organic light emitting diode industry. Nevertheless, a comprehensive description of charge transport in the presence of doping is still missing for organic semiconductors with localized electronic states. Here, we present a theoretical and computational approach based on percolation theory and quantitatively predict experimental results from the literature for the archetype small molecule materials ZnPc, F8ZnPc and C60. We show that transport in the complex potential landscape that emerges from the presence of localized charges can be aptly analyzed by focusing on the network properties of transport paths instead of just the critical resistance. Specifically, we compute the activation energy of conductivity and the Seebeck energy and yield excellent agreement with experimental data. The previously unexplained increase of the activation energy at high doping concentrations can be clarified by our approach.
Plasma membrane H+ pump at a crossroads of acidic and iron stresses in yeast-to-hypha transition
Authors: Cogo Antônio Jesus Dorighetto , Façanha Arnoldo Rocha , da Silva Teixeira Layz Ribeiro , de Souza Sávio Bastos , da Rocha Janiélio Gonçalves , Figueira Frederico Firme , Eutrópio Frederico Jacob , Bertolazi Amanda Azevedo , de Rezende Carlos Eduardo , Krohling Cesar Abel , Okorokov Lev A , Cruz Cristina , Ramos Alessandro Coutinho , Okorokova-Façanha Anna L 
Published: November 2020

Abstract
Iron is an essential nutrient but is toxic in excess mainly under acidic conditions. Yeasts have emerged as low cost, highly efficient soil inoculants for the decontamination of metal-polluted areas, harnessing an increasing understanding of their metal tolerance mechanisms. Here, we investigated the effects of extracellular iron and acid pH stress on the dimorphism of Yarrowia lipolytica. Its growth was unaffected by 1 or 2 mM FeSO4, while a strong cellular iron accumulation was detected. However, the iron treatments decreased the hyphal length and number, mainly at 2 mM FeSO4 and pH 4.5. Inward cell membrane H+ fluxes were found at pH 4.5 and 6.0 correlated with a pH increase at the cell surface and a conspicuous yeast-to-hypha transition activity. Conversely, a remarkable H+ efflux was detected at pH 3.0, related to the extracellular microenvironment acidification and inhibition of yeast-to-hypha transition. Iron treatments intensified H+ influxes at pH 4.5 and 6.0 and inhibited H+ efflux at pH 3.0. Moreover, iron treatments inhibited the expression and activities of the plasma membrane H+-ATPase, with the H+ transport inhibited to a greater extent than the ATP hydrolysis, suggesting an iron-induced uncoupling of the pump. Our data indicate that Y. lipolytica adaptations to high iron and acidic environments occur at the expense of remodelling the yeast morphogenesis through a cellular pH modulation by H+-ATPases and H+ coupled transporters, highlighting the capacity of this non-conventional yeast to accumulate high amounts of iron and its potential application for bioremediation.
Evolution of the truncated disc and inner hot-flow of GX 339–4
Authors: Chainakun P. , Luangtip W. , Young A. J. , Thongkonsing P. , Srichok M. 
Published: November 2020

Aims. We study the changes in geometry of the truncated disc and the inner hot-flow of GX 339–4 by analysing the power spectral density (PSD) extracted from six XMM-Newton observations taken at the very end of an outburst.
Methods. We developed a theoretical model of the PSD of GX 339–4 in the 0.3–0.7 keV (thermal-reverberation-dominated) and 0.7–1.5 keV (disc-continuum-dominated) energy bands. The model assumes the standard accretion disc to be truncated at a specific radius, inside of which are two distinct hot-flow zones: one spectrally soft and the other spectrally hard. The effects of disc fluctuations and thermal reverberation are taken into account.
Results. This model successfully produces the traditional bumpy PSD profiles and provides good fits to the GX 339–4 data. The truncation radius is found to increase from rtrc ∼ 10 to 55rg as the source luminosity decreases, confirming that the truncation radius can be characterized as a function of luminosity. Keeping in mind the large uncertainty in previous measurements of the truncation radius, our values are larger than some obtained from spectroscopic analysis, but smaller than those implied by reverberation lag analysis. Furthermore, the size of two inner hot-flow zones that are spectrally hard and spectrally soft also increases from ∼5 to 27rg and from ∼3 to 26rg, respectively, as the flux decreases. We find that the radial range of the inner hard zone is always larger than the range of the soft hot-flow zone, but by a comparatively small factor of ∼1.1–2.2
Physics of ULIRGs with MUSE and ALMA: The PUMA project
Authors: Perna M. , Arribas S. , Pereira Santaella M. , Colina L. , Bellocchi E. , Catalán-Torrecilla C. , Cazzoli S. , Crespo Gómez A. , Maiolino R. , Piqueras López J. , Rodríguez del Pino B. 
Published: November 2020

Context. Ultraluminous infrared galaxies (ULIRGs) are characterised by extreme starburst (SB) and active galactic nucleus (AGN) activity, and are therefore ideal laboratories for studying the outflow phenomena and their feedback effects. We have recently started a project called Physics of ULIRGs with MUSE and ALMA (PUMA), which is a survey of 25 nearby (z < 0.165) ULIRGs observed with the integral field spectrograph MUSE and the interferometer ALMA. This sample includes systems with both AGN and SB nuclear activity in the pre- and post-coalescence phases of major mergers.
Aims. The main goals of the project are (i) to study the prevalence of (ionised, neutral, and molecular) outflows as a function of the galaxy properties, (ii) to constrain the driving mechanisms of the outflows (e.g. distinguish between SB and AGN winds), and (iii) to identify and characterise feedback effects on the host galaxy. In this first paper, we present details on the sample selection, MUSE observations, and data reduction, and derive first high-level data products.
Methods. MUSE data cubes were analysed to study the dynamical status of each of the 21 ULIRGs observed so far, taking the stellar kinematics and the morphological properties inferred from MUSE narrow-band images into account. We also located the ULIRG nuclei, taking advantage of near-infrared (HST) and millimeter (ALMA) data, and studied their optical spectra to infer (i) the ionisation state through standard optical line ratio diagnostics, and (ii) outflows in both atomic ionised ([O III], Hα) and neutral (Na ID) gas.
Results. We show that the morphological and stellar kinematic classifications are consistent: post-coalescence systems are more likely associated with ordered motions, while interacting (binary) systems are dominated by non-ordered and streaming motions. We also find broad and asymmetric [O III] and Na ID profiles in almost all nuclear spectra, with line widths in the range [300 − 2000] km s−1, possibly associated with AGN- and SB-driven winds. This result reinforces previous findings that indicated that outflows are ubiquitous during the pre- and post-coalescence phases of major mergers.
Neutral-Eosin Y-Catalyzed Regioselective Hydroacylation of Aryl Alkenes under Visible-Light Irradiation
Authors: Tang Xinxin , Wu Jie , Liu Haiwang , Xue Fei , Wang Mu 
Published: November 2020

AbstractStyrene derivatives were hydroacylated with exclusive anti-Markovnikov selectivity by using neutral eosin Y as a direct hydrogen-atom-transfer (HAT) catalyst under visible-light irradiation. Aldehydes and styrenes with various substituents were tolerated (>20 examples), giving the corresponding products in moderate to high yields. The key acyl radical intermediate was generated from a direct HAT process induced by photoexcited eosin Y. Subsequent addition to styrenes and a reverse HAT process generated the ketone products.
Diagnostic Value of Cholinesterase Activity for the Development of Postoperative Delirium after Cardiac Surgery
Authors: Saha Shekhar , Karaca Kübra , Jebran Ahmad Fawad , Waezi Narges , Ort Katharina , Brandes Ivo , Hagl Christian , Niehaus Heidi 
Published: November 2020

Abstract
Background Depression of cholinesterase (CHE) activity has been reported to lead to an amplified neuroinflammatory response, which clinically manifests as postoperative delirium (PD). This observational study investigates the association between CHE activity and the development of PD following elective cardiac surgery.
Methods Patients with preexisting neurologic deficits or carotid artery disease as well as patients undergoing reoperations or procedures under circulatory arrest have been excluded from this study. The Mini-Mental State Examination, the Confusion Assessment Method for the Intensive Care Unit, and the Intensive Care Delirium Screening Checklist were performed at regular intervals. CHE activity was estimated pre- and postoperatively until postoperative day (POD) 5 and at discharge.
Results A total of 107 patients were included. PD was diagnosed in 34 (31.8%) patients, who have been compared with those without PD. Time on ventilator, length of ICU, and hospital stay were longer in patients with PD (p = 0.001, p < 0.001, and p = 0.004, respectively). MMSE scores were lower in patients with PD (p < 0.001; p = 0.015). CHE activity on POD 1 to 4 as well as at discharge were lower in the delirium group (p = 0.041; p = 0.029; p = 0.015; p = 0.035; p = 0.028, respectively). A perioperative drop of CHE activity of more than 50% and a postoperative CHE activity below 4,800 U/L (on POD 0) were independently associated with an increased risk of development of PD (p = 0.038; p = 0.008, respectively).
Conclusion In addition to the established functional tests, routine estimation of CHE activity may serve as an additional diagnostic tool allowing for the timely diagnosis and treatment of PD in cardiac surgery patients.
Computed Tomography-Guided Trans-scapular Coil Localization for Pulmonary Nodules
Authors: Zhang Jian-Hua , Zhou Shi-Qing , Xia Feng-Fei , Wang Tao 
Published: November 2020

Abstract
Background The aim of the study is to evaluate the feasibility, safety, and effectiveness of preoperative computed tomography (CT)-guided trans-scapular coil localization (TSCL) of scapula-blocked pulmonary nodules (PNs).
Methods Between November 2015 and May 2020, 11 patients underwent preoperative CT-guided TSCL procedures owing to PN occlusion by scapula.
Results A 100% technical success rate was achieved for CT-guided TSCL, with one coil being used for each PN. One patient (9.1%) developed pneumothorax. Successful video-assisted thoracoscopic surgery (VATS)-guided wedge resection of these scapula-blocked PNs was conducted in all patients.
Conclusion CT-guided TSCL can be simply and safely used to facilitate successful VATS-guided wedge resection of scapula-blocked PNs.
The Role of the Heart Team in Patients with Diffuse Coronary Artery Disease Undergoing Coronary Artery Bypass Grafting
Authors: Dourado Luciana Oliveira Cascaes , Pereira Alexandre C. , Poppi Nilson Tavares , Cavalcante Rafael , Gaiotto Fabio , Dallan Luis Alberto Oliveira , Bittencourt Marcio Sommer , Cesar Luiz Antonio M. , Gowdak Luis Henrique W. 
Published: November 2020

Abstract
Background In patients eligible for coronary artery bypass grafting, no data assess the importance of the Heart Team in programming the best surgical strategy for patients with diffuse coronary artery disease (CAD). This study aims to determine the contribution of the Heart Team in predicting the feasibility of coronary artery bypass graft and angiographic surgical success in these patients based on visual angiographic analysis.
Methods Patients with diffuse and severe CAD undergoing incomplete coronary artery bypass graft surgery were prospectively included. One-year postoperative coronary angiograms were obtained to evaluate graft occlusion. Two clinical cardiologists, two cardiovascular surgeons, and one interventional cardiologist retrospectively analyzed preoperative angiograms. A subjective scale was applied at a single moment to quantify the chance of successful coronary artery bypass grafting for each coronary territory with anatomical indication for revascularization. Based on individual scores, the Heart Team's and the specialists' scores were calculated and compared.
Results The examiners evaluated 154 coronary territories, of which 85 (55.2%) were protected. The Heart Team's accuracy for predicting the angiographic success of the surgery was 74.9%, almost equal to that of the surgeons alone (73.2%). Only the interventional cardiologist predicted left anterior descending territory grafting success. The Heart Team had good specificity and reasonable sensitivity, and the surgeons had high sensitivity and low specificity in predicting angiographic success.
Conclusion The multispecialty Heart Team achieved good accuracy in predicting the angiographic coronary artery bypass graft success in patients with diffuse CAD, with a high specificity and reasonable sensitivity.