bluuebunny/crossref_metadata_2025_split huggingface dataset:
Resveratrol stereoselectively affected (±)warfarin pharmacokinetics and enhanced the anticoagulation effect
Authors: Huang Tse-Yin , Yu Chung-Ping , Hsieh Yow-Wen , Lin Shiuan-Pey , Hou Yu-Chi 
Published: September 2020

AbstractResveratrol (RVT) has various beneficial bioactivities and popularly used as a dietary supplement. RVT showed inhibitions on CYP1A2/2C9/3A4, breast cancer resistance protein (BCRP), and some conjugated metabolites of RVT also inhibited BCRP. (±)Warfarin, an anticoagulant for cardiovascular disease but with narrow therapeutic window, were substrates of CYP1A2/3A4(R-form), 2C9(S-form) and BCRP. We hypothesized that the concurrent use of RVT might affect the metabolism and excretion of warfarin. This study investigated the effect of RVT on the pharmacokinetics and anticoagulation effect of (±)warfarin. Rats were orally given (±)warfarin (0.2 mg/kg) without and with RVT (100 mg/kg) in a parallel design. The results showed that RVT significantly increased the AUC0−t of S-warfarin and international normalized ratio. Mechanism studies showed that both RVT and its serum metabolites (RSM) inhibited BCRP-mediated efflux of R- and S-warfarin. Moreover, RSM activated CYP1A2/3A4, but inhibited CYP2C9. In conclusion, concomitant intake of RVT increased the systemic exposure of warfarin and enhanced the anticoagulation effect mainly via inhibitions on BCRP and CYP2C9.
Structure-resistive property relationships in thin ferroelectric BaTiO$$_{3}$$ films
Authors: Andreeva N. V. , Petraru A. , Vilkov O. Yu. , Petukhov A. E. 
Published: September 2020

AbstractA combined study of local structural, electric and ferroelectric properties of SrTiO$$_{3}$$

3

/La$$_{0.7}$$

0.7

Sr$$_{0.3}$$

0.3

MnO$$_{3}$$

3

/BaTiO$$_{3}$$

3

heterostructures was performed by Piezoresponse Force Microscopy, tunneling Atomic Force Microscopy and Scanning Tunneling Microscopy in the temperature range 30–295 K. The direct correlation of film structure (epitaxial, nanocrystalline or polycrystalline) with local electric and ferroelectric properties was observed. For polycrystalline ferroelectric films the predominant polarization state is defined by the peculiarity of screening the built-in field by positively charged point defects. Based on Scanning Tunneling Spectroscopy results, it was found that a sequent voltage application provokes the modification of local resistive properties related to the redistribution of point defects in thin ferroelectric films. A qualitative analysis of acquired Piezoresponse Force Microscopy, tunneling Atomic Force Microscopy and Scanning Tunneling Microscopy images together with Scanning Tunneling Spectroscopy measurements enabled us to conclude that in the presence of structural defects the competing processes of electron injection, trap filling and the drift of positively charged point defects drives the change of resistive properties of thin films under applied electric field. In this paper, we propose a new approach based on Scanning Tunneling Microscopy/Spectroscopy under ultrahigh vacuum conditions to clarify the influence of point defects on local resistive properties of nanometer-thick ferroelectric films.
Strain-specific strategies of 2′-fucosyllactose, 3-fucosyllactose, and difucosyllactose assimilation by Bifidobacterium longum subsp. infantis Bi-26 and ATCC 15697
Authors: Zabel Bryan E. , Gerdes Svetlana , Evans Kara C. , Nedveck Derek , Singles Suzanne Koch , Volk Barbara , Budinoff Charles 
Published: September 2020

AbstractHuman milk provides essential nutrients for infant nutrition. A large proportion of human milk is composed of human milk oligosaccharides (HMOs), which are resistant to digestion by the infant. Instead, HMOs act as a bioactive and prebiotic enriching HMO-utilizing bacteria and cause systematic changes in the host. Several species ofBifidobacteriumhave been shown to utilize HMOs by conserved, as well as species-specific pathways, but less work has been done to study variation within species or sub-species.B. longumsubsp.infantisis a prevalent species in the breast-fed infant gut and the molecular mechanisms of HMO utilization for the type strainB. longumsubsp.infantisATCC 15697 (type strain) have been well characterized. We used growth, transcriptomic, and metabolite analysis to characterize key differences in the utilization of 2′FL, 3FL and DFL (FLs) betweenB. longumsubsp.infantisBi-26 (Bi-26) and the type strain. Bi-26 grows faster, produces unique metabolites, and has a distinct global gene transcription response to FLs compared to the type strain. Taken together the findings demonstrate major strain specific adaptations in Bi-26 to efficient utilization of FLs.
Infralimbic cortex controls fear memory generalization and susceptibility to extinction during consolidation
Authors: Bayer Hugo , Bertoglio Leandro Jose 
Published: September 2020

AbstractLesioning or inactivating the infralimbic (IL) subregion of the medial prefrontal cortex before acquisition produces more generalized and extinction-resistant fear memories. However, whether and how it modulates memory specificity and extinction susceptibility while consolidation takes place is still unknown. The present study aims to investigate these questions using muscimol-induced temporary inactivation and anisomycin-induced protein synthesis inhibition in the rat IL following contextual fear conditioning. Results indicate that the IL activity immediately after acquisition, but not six hours later, controls memory generalization over a week, regardless of its strength. Such IL function depends on the context-shock pairing since muscimol induced no changes in animals exposed to immediate shocks or the conditioning context only. Animals in which the IL was inactivated during consolidation extinguished similarly to controls within the session but were unable to recall the extinction memory the following day. Noteworthy, these post-acquisition IL inactivation-induced effects were not associated with changes in anxiety, as assessed in the elevated plus-maze test. Anisomycin results indicate that the IL protein synthesis during consolidation contributes more to producing extinction-sensitive fear memories than memory specificity. Collectively, present results provide evidence for the IL's role in controlling generalization and susceptibility to extinction during fear memory consolidation.
Urban resources limit pair coordination over offspring provisioning
Authors: Baldan Davide , Ouyang Jenny Q. 
Published: September 2020

AbstractThe amount of care parents provide to the offspring is complicated by an evolutionary conflict of interest (‘sexual conflict’) between the two parents. Recent theoretical models suggest that pair coordination of the provisioning may reduce this conflict and increase parent and offspring fitness. Despite empirical studies showing that pair coordination is common in avian species, it remains unclear how environmental and ecological conditions might promote or limit the ability of parents to coordinate care. We compared the level of pair coordination, measured as alternation and synchrony of the nest visits, of house wrens Troglodytes aedon pairs breeding in a rural (10 nests) and a suburban (9 nests) site and investigated how differences in parental behaviours were related to habitat composition, prey abundance and how they ultimately related to reproductive success. We found that parents alternated and synchronized their nest visits more in the rural site compared to the suburban one. The suburban site is characterized by a more fragmented habitat with more coniferous trees and less caterpillar availability. Offspring from the rural site were heavier at fledging than at the suburban site. Taken together, these results suggest that environmental conditions play an important role on the emergence of coordinated parental care and that considering environmental variables is pivotal to assess the fitness consequences of parental strategies.
Ambrisentan, an endothelin receptor type A-selective antagonist, inhibits cancer cell migration, invasion, and metastasis
Authors: Kappes Lucy , Amer Ruba L. , Sommerlatte Sabine , Bashir Ghada , Plattfaut Corinna , Gieseler Frank , Gemoll Timo , Busch Hauke , Altahrawi Abeer , Al-Sbiei Ashraf , Haneefa Shoja M. , Arafat Kholoud , Schimke Lena F. , Khawanky Nadia El , Schulze-Forster Kai , Heidecke Harald , Kerstein-Staehle Anja , Marschner Gabriele , Pitann Silke , Ochs Hans D. , Mueller Antje , Attoub Samir , Fernandez-Cabezudo Maria J. , Riemekasten Gabriela , al-Ramadi Basel K. , Cabral-Marques Otavio 
Published: September 2020

AbstractSeveral studies reported a central role of the endothelin type A receptor (ETAR) in tumor progression leading to the formation of metastasis. Here, we investigated the in vitro and in vivo anti-tumor effects of the FDA-approved ETAR antagonist, Ambrisentan, which is currently used to treat patients with pulmonary arterial hypertension. In vitro, Ambrisentan inhibited both spontaneous and induced migration/invasion capacity of different tumor cells (COLO-357 metastatic pancreatic adenocarcinoma, OvCar3 ovarian carcinoma, MDA-MB-231 breast adenocarcinoma, and HL-60 promyelocytic leukemia). Whole transcriptome analysis using RNAseq indicated Ambrisentan’s inhibitory effects on the whole transcriptome of resting and PAR2-activated COLO-357 cells, which tended to normalize to an unstimulated profile. Finally, in a pre-clinical murine model of metastatic breast cancer, treatment with Ambrisentan was effective in decreasing metastasis into the lungs and liver. Importantly, this was associated with a significant enhancement in animal survival. Taken together, our work suggests a new therapeutic application for Ambrisentan in the treatment of cancer metastasis.
A simple procedure for bacterial expression and purification of the fragile X protein family
Authors: Edwards Madison , Xu Mingzhi , Joseph Simpson 
Published: September 2020

AbstractThe fragile X protein family consists of three RNA-binding proteins involved in translational regulation. Fragile X mental retardation protein (FMRP) is well-studied, as its loss leads to fragile X syndrome, a neurodevelopmental disorder which is the most prevalent form of inherited mental retardation and the primary monogenetic cause of autism. Fragile X related proteins 1 and 2 (FXR1P and FXR2P) are autosomal paralogs of FMRP that are involved in promoting muscle development and neural development, respectively. There is great interest in studying this family of proteins, yet researchers have faced much difficulty in expressing and purifying the full-length versions of these proteins in sufficient quantities. We have developed a simple, rapid, and inexpensive procedure that allows for the recombinant expression and purification of full-length human FMRP, FXR1P, and FXR2P from Escherichia coli in high yields, free of protein and nucleic acid contamination. In order to assess the proteins’ function after purification, we confirmed their binding to pseudoknot and G-quadruplex forming RNAs as well as their ability to regulate translation in vitro.
Kava constituents exert selective anticancer effects in oral squamous cell carcinoma cells in vitro
Authors: Celentano Antonio , Yiannis Callisthenis , Paolini Rita , Zhang Pangzhen , Farah Camile S. , Cirillo Nicola , Yap Tami , McCullough Michael 
Published: September 2020

AbstractKava is a beverage made from the ground roots of the plant Piper Methysticum. Active compounds of Kava have previously been demonstrated to exert an antiproliferative effect through cell cycle arrest and promotion of apoptosis. Our aim was to investigate the in vitro effects of the main constituents derived from Kava on oral squamous cell carcinoma (OSCC) activity.
Gas chromatography mass spectrometry (GCMS) was used to characterise the main constituents of two Kava preparations. Cell proliferation was assessed in two human OSCC cell lines (H400 and BICR56) and in normal oral keratinocytes (OKF6) treated with the identified Kava constituents, namely Flavokawain A (FKA), Flavokawain B (FKB), yangonin, kavain and methysticin using an MTS in vitro assay. Cell migration at 16 h was assessed using a Transwell migration assay. Cell invasion was measured at 22 h using a Matrigel assay. Cell adhesion was assessed at 90 min with a Cytoselect Adhesion assay. The two Kava preparations contained substantially different concentrations of the main chemical constituents. Treatment of malignant and normal oral keratinocyte cell lines with three of the identified constituents, 10 μg/ml FKA, 2.5 μg/ml FKB and 10 μg/ml yangonin, showed a significant reduction in cell proliferation in both H400 and BICR56 cancer cell lines but not in normal OKF6 cells. Remarkably, the same Kava constituents induced a significant reduction of OSCC cell migration and invasion. We have demonstrated, for the first time, that Kava constituents, FKA, FKB and yangonin have potential anticancer effects on OSCC. This highlights an avenue for further research of Kava constituents in the development of future cancer therapies to prevent and treat OSCC.
Re-evaluation of serum leptin and adiponectin concentrations normalized by body fat mass in patients with rheumatoid arthritis
Authors: Chihara Kazuhisa , Hattori Naoki , Ichikawa Norihiro , Matsuda Takeshi , Saito Takanori 
Published: September 2020

AbstractLeptin and adiponectin are produced mainly in adipocytes and classified as adipocytokines because of their possible involvement in inflammation and immunity. The aim of this study was to elucidate the relationships of these adipocytokines with the disease activities of RA. We examined leptin and adiponectin concentrations and inflammatory markers such as metalloproteinase-3 (MMP-3) in 136 patients with rheumatoid arthritis (RA) (26 males and 110 females, 69.6 ± 9.3 years) and 78 controls (36 males and 42 females, 66.7 ± 15.0 years). Serum leptin and adiponectin concentrations correlated positively (r = 0.565, P < 0.001) and negatively (r = –0.331, P < 0.001) to the amount of body fat, respectively. Serum leptin and adiponectin concentrations normalized by body fat mass were significantly higher in RA than those in controls [leptin, 1.24 (median) ng/mL/kg fat in RA vs. 0.76 ng/mL/kg fat in controls; adiponectin, 0.74 μg/mL/kg fat in RA vs. 0.44 μg/mL/kg fat in controls]. Normalized adiponectin concentrations correlated positively not only to the degree of bone destruction in Steinbrocker classification but also to serum MMP-3 concentrations. Normalized leptin concentrations did not correlate to the degree of bone destruction. We conclude that adiponectin but not leptin may be involved in joint damage in RA.
Dietary flavone from the Tetrastigma hemsleyanum vine triggers human lung adenocarcinoma apoptosis via autophagy
Authors: Li Yonglu , Feng Xinyu , Zhang Yiru , Wang Yaxuan , Yu Xin , Jia Ruoyi , Yu Ting , Zheng Xiaodong , Chu Qiang 
Published: September 2020

A mouse, a cancer-combating winner, is giving his speech about his experience in conquering cancer. He excitedly introduces that TVF, flavones derived from Tetrastigma hemsleyanum vine, effectively achieved tumor ablation.
Phoebe’s differentiated interior from refined shape analysis
Authors: Rambaux N. , Castillo-Rogez J. C. 
Published: September 2020

Context.Phoebe is an irregular satellite of Saturn, and its origin, from either between the orbits of the giant planets or the Kuiper Belt, is still uncertain. The extent of differentiation of its interior can potentially help inform its formation location because it is mainly determined by heat from 26-aluminum. The internal structure is reflected in the shape, assuming the body is relaxed to hydrostatic equilibrium. Although previous data analysis indicates Phoebe is close to hydrostatic equilibrium, its heavily cratered surface makes it difficult to tease out its low-order shape characteristics.Aims.This paper aims to extract Phoebe’s global shape from the observations returned by the Cassini mission for comparison with uniform and stratified interior models under the assumption of hydrostatic equilibrium.Methods.The global shape is derived from fitting spherical harmonics and keeping only the low-degree harmonics that represent the shape underneath the heavily cratered surface. The hydrostatic theoretical model for shape interpretation is based on the Clairaut equation developed to the third order (although the second order is sufficient in this case).Results.We show that Phoebe is differentiated with a mantle density between 1900 and 2400 kg m−3. The presence of a porous surface layer further restricts the fit with the observed shape. This result confirms the earlier suggestion that Phoebe accreted with sufficient 26-aluminium to drive at least partial differentiation, favoring an origin with C-type asteroids.
Efficacy of Metformin and Chemotherapeutic Agents on the Inhibition of Colony Formation and Shh/Gli1 Pathway: Metformin/Docetaxel Versus Metformin/5-Fluorouracil
Authors: Fatehi-Agdam Maryam , Vatankhah Mohammad Amin , Panahizadeh Reza , Jeddi Farhad , Najafzadeh Nowruz 
Published: September 2020

Abstract
Background Gastric cancer is a common gastrointestinal cancer characterized by poor prognosis and chemoresistance. Docetaxel and 5-fluorouracil (5-FU) are frequently used for the treatment of gastric cancer. Despite their potent anti-cancer effects, chemoresistance occurs in metastatic gastric cancer. Metformin, a popular anti-diabetic drug, has been proven to have potent anticancer effects on gastrointestinal cancers. Here, we aim to improve this chemotherapy agents’ efficacy by pretreatment with metformin.
Methods The AGS gastric cancer cell line were pretreated with three different sub-toxic concentration of metformin and then treated with various concentrations of 5-FU and docetaxel.The anticancer effects of the combination of metformin with the chemotherapy agents were determined using clonogenic assay and DAPi staining. We used real-time PCR to evaluate Gli1, Gli2, and TWIST1 mRNA expression levels in the gastric cancer cells. Also, the expression of the Shh protein was assessed using immunocytochemistry.
Results Here, we found that metformin sensitized the gastric cancer cells to chemotherapy. The combination treatments were more effective in reducing the number of cancer colonies compared to 5-FU or docetaxel alone. The combination of metformin with 5-FU or docetaxel significantly reduced the number of cells expressing the Shh protein compared to the 5-FU alone or docetaxel alone. Interestingly, we found that the combination of metformin with docetaxel significantly down-regulated the mRNA levels of Gli1, Gli2, and TWIST1 in the AGS gastric cancer cell line compared to docetaxel alone.
Conclusion Overall, our data strongly support an important role for metformin as an enhancer of the efficacy of chemotherapeutic agents against gastric cancer.
Cerebellopontine Angle Epidermoid Cysts: Surgical Treatment Outcome
Authors: Refaat Mohamed I. , Abdallah Omar Y. 
Published: September 2020

Abstract
Background  Epidermoid cysts (ECs)are an uncommon slowly growing congenital lesions. The cerebellopontine angle (CPA) is the most common site. Surgical management for these lesions remains challenging due to the high-adherence of the cyst capsule to the surrounding structures and its tendency to present with invasion of multiple cisterns.
Objective The aim of this study was to evaluate the clinical and radiological outcome of surgically treated ECs in the CPA region.
Materials and Methods This is a retrospective study conducted on patients presenting with symptomatic CPA ECs. Clinical outcome was assessed in terms of improving or worsening of the presenting symptoms. Radiological outcome was assessed by comparing the tumor size, brainstem, and cerebellar compression in the preoperative and postoperative images.
Results In this study, 18 patients were included. All cases were operated upon by microsurgical evacuation of the cyst contents and excision of the nonadherent parts of the capsule. On admission, cranial nerve symptoms predominated. Improvement or resolution of preoperative deficits was seen in 15 patients. Preoperative symptoms worsened in two and new postoperative symptoms occurred in one patient. Seven patients needed additional ventriculoperitoneal (VP) shunt in the same setting due to the presence of hydrocephalus. During the follow-up period, symptomatic recurrence occurred in one patient, while asymptomatic recurrence occurred in one patient.
Conclusion Surgical management of CPA ECs, leaving remnants of the capsule on cranial nerves and brain stem can be considered as a relatively safe surgical procedure with a good clinical outcome and low-recurrence risks.
Morphological structure can escape reduction effects from mass admixture of second language speakers
Authors: Widmer Manuel , Jenny Mathias , Behr Wolfgang , Bickel Balthasar 
Published: September 2020

AbstractMorphological complexity is expected to decrease under mass admixture from adult second language speakers. While this has been chiefly shown for morphological richness, an unresolved question is whether the effect extends to aspects of morphological boundedness. Here we report a case study of Sino-Tibetan verbs, contrasting verbal expressions of two languages with very large (Chinese, Burmese) and of two languages with very small (Bunan, Chintang) numbers of second language speakers. We find that while the amount of second language speakers accounts for differences in the range and number of inflectional categories (degrees of synthesis), it does not affect the way in which morphological constituents are bound together, reflecting fortification through a mix of diachronically stable and universally preferred patterns. This calls for theoretical models that narrow down the range of changes that are driven by second language speaker admixture, and for extensive empirical testing on a global scale.
Design of CMOS active pixels based on finger-shaped PPD
Authors: Li Feng , Wang Ruishuo , Han Liqiang , Xu Jiangtao 
Published: September 2020

Abstract
To improve the full-well capacity and linear dynamic range of CMOS image sensor, a special finger-shaped pinned photodiode (PPD) is designed. In terms of process, the first N-type ion implantation of the PPD N buried layer is extended under the transfer gate, thereby increasing the PPD capacitance. Based on TCAD simulation, the width and spacing of PPD were precisely adjusted. A high full-well capacity pixel design with a pixel size of 6 × 6 μm2 is realized based on the 0.18 μm CMOS process. The simulation results indicate that the pixel with the above structure and process has a depletion depth of 2.8 μm and a charge transfer efficiency of 100%. The measurement results of the test chip show that the full-well capacity can reach 68650e–. Compared with the conventional structure, the proposed PPD structure can effectively improve the full well capacity of the pixel.
Suppression of oxygen and carbon impurity deposition in the thermal system of Czochralski monocrystalline silicon
Authors: Zhang Jing , Liu Ding , Pan Yani 
Published: September 2020

Abstract
When preparing large monocrystalline silicon materials, severe carbon etching and silicide deposition often occur to the thermal system. Therefore, a suppression method that optimizes the upper insulation structure has been proposed. Assisted by the finite element method, we calculated temperature distribution and carbon deposition of heater and heat shield, made the rule of silicide and temperature distributing in the system, and we explained the formation of impurity deposition. Our results show that the optimized thermal system reduces carbon etching loss on heat components. The lowered pressure of the furnace brings a rapid decrease of silicide deposition. The increase of the argon flow rate effectively inhibits CO and back diffusion. The simulated results agree well with the experiment observations, validating the effectiveness of the proposed method.