bluuebunny/crossref_metadata_2025_split huggingface dataset:
Psychological Investigation on Pregnant Women during the Outbreak of COVID-19
Authors: Chen Shaoqi , Zhuang Jiamian , Chen Qingzi , Tan Xuerui 
Published: June 2020

Abstract
Background COVID-19(Corona Virus Disease 2019) outbreaks around the world and is highly infectious, which may cause people prone to anxiety and depression. As a particular group, pregnant women need more attention. The aim of study is to investigate the mental health status of pregnant women during the outbreak of COVID-19, and analyze factors affecting the mental health status of pregnant women.Methods Using a self-designed questionnaire, self-rated anxiety scale(SAS), self-rated depression scale (SDS), we conducted a web-based survey on 1160 pregnant women during the outbreak of COVID-19.Results Compared with general adults in some regions of China, the scores of SAS and SDS were both significantly higher in pregnant women during the outbreak of COVID-19 (P < 0.05). The results of multivariate regression analysis unveiled that age, levels of education, and duration of pregnancy were all factors influencing pregnant women’s psychological status. In terms of psychological problems, compared with pregnant women aged < 30 years old, the risk of psychological problems in pregnant women aged ≥ 30 years old was 0.646 times (95% confidence interval (CI): 0.486–0.858). Moreover, compared with women with a level of high school or below, the risk of psychological abnormalities in women with a level of junior college degree or above was 0.551 times (95% CI: 0.416–0.731). Compared with women during the first trimester, the risk of psychological problems in women during the mid-trimester was 0.543 times (95% CI: 0.398–0.739). In addition, compared with women during the mid-trimester, the risk of psychological problems in women during the last trimester was 0.636 times (95% CI: 0.466–0.867).Conclusions During the outbreak of COVID-19, pregnant women are prone to anxiety or depression, highlighting the necessity of further attention on those subjects. It is of great significance to provide on-time psychological intervention and psychological counseling for pregnant women with poor mental health.
Preliminary experience with tacrolimus in managing Henoch-Schönlein purpura featuring extrarenal symptoms refractory to conventional therapy
Authors: Chen Yan , Bai Haitao , Yang Yang , Lian Zhulan 
Published: June 2020

Abstract
Background

Henoch-Schönlein purpura (HSP) is the commonest vasculitis of childhood, affecting multiple organ systems. The extrarenal manifestations are prominent in some HSP cases, for which no standard treatment is available. It is interesting to examine the value of tacrolimus in such cases.
Findings

Seventeen children received tacrolimus to manage their refractory extrarenal symptoms of HSP in an open-label prospective study. Tacrolimus was administered at daily dose of 0.05–0.15 mg/kg. Tacrolimus was effective in all cases after treatment for 7–21 days. Tacrolimus treatment resulted in complete resolution of extrarenal symptoms in 15 cases, and partial resolution in the remaining 2 cases. During follow-up, skin purpura reoccurred in 2 cases, abdominal pain and loss of follow-up in 1 case each. Drug-related adverse reaction was not observed in any case. Abnormal renal function test was identified in 7 patients by urinary screening tests before tacrolimus therapy, 4 of them received renal biopsy. Only one patient showed evidence of renal impairment during follow-up.
Conclusions

Tacrolimus works quickly without drug-related adverse reaction in managing the HSP patients featuring recurrent extrarenal symptoms. Our preliminary experience suggests good clinical efficacy and safety of tacrolimus in such off-label use.
Medication-related problems among hospitalized pregnant women in a tertiary teaching hospital in Ethiopia: A prospective observational study
Authors: AHMED SEID MUSSA , Sundby Johanne , Aragaw Yesuf Ahmed , Nordeng Hedvig 
Published: June 2020

Abstract
Background
Studies on medication-related problems (MRPs) among pregnant women are scarce, despite the potential consequences for both mother and child. This study aimed to describe the prevalence, clinical significance, and risk factors for MRPs among hospitalized pregnant or postpartum women at Jimma University Medical Centre (JUMC) in Ethiopia.
Methods
A prospective follow-up and clinical audit of 1,117 hospitalized pregnant or postpartum women in the maternity and gynaecology wards at JUMC was carried out between February and June 2017. Patients were followed throughout their stay in the hospital to assess the presence and development of MRPs. Pre-tested data extraction form and an interview-guided structured questionnaire were used to collect data. Descriptive statistics were used to describe MRPs. Logistic regression analysis was used to identify factors associated with MRPs.
Results
One or more MRPs occurred among 323 (28.9%) study participants, mostly in relation to lack of iron supplementation. A total of 278 (70.6%) of all MRPs were considered to be of moderate to high clinical significance. Nulliparous women were more likely to experience MRPs than their counterparts (adjusted OR 1.82; 95% CI 1.23, 2.69). When excluding MRPs due to iron from the analysis, nulliparous women (adjusted OR 1.99; 95% CI 1.22, 3.24) and previous medication use were significantly associated with experiencing an MRP (adjusted OR 2.38; 95% CI 1.24, 4.56).
Conclusions
Nearly 3 out of 10 hospitalized pregnant women at JUMC had one or more MRPs. The need for additional iron therapy was by far the most common type of MRP. Improved adherence to guidelines on iron supplementation are required. Multidisciplinary approaches including physicians, nurses, and clinical pharmacists in the maternity and gynaecology wards could possibly prevent MRPs and promote patient safety for women and children.
Integrated analysis of the functions and prognostic values of RNA binding proteins in colon adenocarcinoma
Authors: Liu Xinhong , Tan Fang , Long Xingyao , Yi Ruokun , Yang Dingyi , Zhao Xin 
Published: June 2020

Abstract
Background

RNA binding proteins (RBPs) play an important role in a variety of cancers. However, the role of RBPs in colorectal adenocarcinoma (COAD) has not been studied. Integrated analysis of RBPs will provide a better understanding of disease genesis and new insights into COAD treatment.
Methods

The gene expression data and corresponding clinical information for COAD were downloaded from The Cancer Genome Atlas (TCGA) database. Univariate Cox regression analysis was used to screen for RBPs associated with COAD recurrence, and multivariate Cox proportional hazards regression analyses were used to identify genes that were associated with COAD recurrence. A nomogram was constructed to predict the recurrence of COAD, and a receiver operating characteristic (ROC) curve analysis was performed to determine the accuracy of the prediction models. The Human Protein Atlas database was used in prediction models to confirm the expression of key genes in COAD patients.
Result

A total of 177 differentially expressed RBPs was obtained, comprising 123 upregulated and 54 downregulated. GO and KEGG enrichment analysis showed that the differentially expressed RBPs were mainly related to mRNA metabolism, RNA processing and translation regulation. Seven RBP genes (TDRD6, POP1, TDRD7, PPARGC1A, LIN28B, LRRFIP2 and PNLDC1) were identified as prognosis-associated genes and were used to construct the prognostic model.
Conclusion

We constructed a COAD prognostic model through bioinformatics analysis, which indicated that prognostic model RBPs have a potential role in the diagnosis and prognosis of COAD. Moreover, the nomogram can effectively predict the 1-year, 3-year, and 5-year survival rate for COAD patients.
Association of Neutrophil-to-Lymphocyte Ratio, Neutrophil Count, and VEGF on Metastasis in Patients with Nasopharyngeal Carcinoma
Authors: Xingming Ye , Lu Tianzhu , Bai Wendong , Jia Jing , Wang Lin , Zong Jingfeng , Chen Ying 
Published: June 2020

Abstract
Background Systemic inflammatory responses play important roles in tumor progression, including metastasis tendency. A high neutrophil-to-lymphocyte ratio (NLR) is associated with poor prognosis in nasopharyngeal carcinoma (NPC), but the underlying mechanisms are not clear. Vascular endothelial growth factor (VEGF) has been reported to be involved in the recruitment of pro-angiogenic neutrophils in tumor metastasis progress. Therefore, we focused on the correlations among NLR, neutrophil count, and VEGF during metastasis and explored the reasons for changes of NLR in advanced NPC.Methods Consecutive patients who had been investigated with magnetic resonance imaging and irradiated with intensity-modulated radiotherapy(IMRT) from November 2011 to December 2012 were studied retrospectively. Clinical parameters (NLR, neutrophil count) were measured and their association with NPC stage advancement and VEGF were assessed using the Kruskal–Wallis test. Survival curves were created using the Kaplan–Meier method and log-rank test.Results NLR was elevated in advanced N stage (P < 0.0001) and M stage disease (P = 0.0034), but not T stage disease (P = 0.7049). Changes to the NLR in advanced N stage and M stage disease were affected most strongly by neutrophil count (P = 0.0074 and P = 0.0064, respectively) and higher NLR levels were associated with higher neutrophil count (P < 0.0001). NLR > 2.26 and positive VEGF expression (+++) correlated significantly with distant metastasis-free survival (P = 0.0340 and P = 0.0023, respectively). Neutrophil counts correlated positively with VEGF expression (P = 0.0024) in NPC tissue.Conclusion Our results demonstrate a positive correlation between NLR, neutrophil count, and VEGF expression in NPC. Higher NLR and VEGF expression are associated with distant metastasis-free survival among patients with NPC, which implies that they set a basis of immune activation in advanced nasopharyngeal carcinoma.
FeS Encapsulated Chitosan Graft Polyacrylamide Nanocomposite for the Uptake of Model Anionic Eosin Y from Water: Isotherm, Kinetics and Equilibrium Studies.
Authors: Danu Bernice Y. , Agorku Eric S. , Ampong Francis K. , Awudza Johannes A.M. , Torve Vincent , Amponsah Caleb , Quaye Ruth N.M , Ama Onoyivwe M. , Osifo Peter O. , Ray Suprakas S. 
Published: June 2020

Abstract
A novel bio-nanocomposite, FeS encapsulated chitosan grafted polyacrylamide, was successfully synthesized using a grafting technique that employs microwave irradiation. Employing FTIR, SEM, XRD, TEM and SAED analysis, the synthesized nanocomposites were characterized. Thermodynamic and kinetic studies were accomplished using the batch mode via the eosin yellow adsorption from aqueous solution using the nanocomposite. The adsorption process was tailored to the pseudo-second-order kinetic model and the Freundlich isotherm model. The enthalpy change, ∆H° (-12.243 kJ/mol), was an exothermic process. The Gibbs energy, ∆G° (from -5.492 kJ/mol.K to -5.078 kJ/mol.K for temperatures from 300 K to 318 K respectively) values were indications of a thermodynamically feasible reaction process which was spontaneous within the temperature domain. The change in entropy, ∆S° (-0.023 kJ/mol), showed reduced randomness during the adsorption process. The mean free energy of adsorption was 0.408 kJ/mol, which was an indication of physisorption. These results show the possible use of the graft copolymer as an adsorbent in water treatment.
Evaluation of the Autof MS 1000 mass spectrometer for identification of clinical isolates
Authors: Ma Qiong , Zhang Qi , Yuan Youhua , Yan Wenjuan , Wan Shanmei , Xu Junhong , Zhang Jiangfeng , Wang Yuming , Li Yi 
Published: June 2020

Abstract
Background: To evaluate the accuracy and performance of Autof MS 1000 mass spectrometer in bacterial and yeasts identification., a total of 2342 isolates were routinely obtained from microbial cultures of clinical specimens (i.e., blood, cerebrospinal fluid, respiratory tract, Puncture fluid, wound, stool and urine) from January to December 2019 in Henan Provincial People’s Hospital. Then, the repetitive strains of the same patient were excluded. The two mass spectrometry methods by Autof MS 1000 and Bruker biotyper systems and the classical biochemical VITEK 2/API 20C AUX Systems testing were used for analysis. The strains with different results by the above three test methods were identified by 16S rDNA and other specific genes sequencing.Results: The accuracies of isolates identification at species level by Autof MS 1000 and Bruker biotyper systems were 98.9% and 98.5%, respectively. The genus level by Autof MS 1000 and Bruker biotyper systems were 99.7% and 99.4%, respectively. The Autof instrument was not significantly different from the Bruker instrument in identification accuracy at both the species and genus levels. The frequencies of unreliable identification were 1.1% (26/2342) for Autof ms1000 and 1.5% (34/2342) for Bruker biotyper, respectively. The results of in vitro experiments demonstrated that the coincidence rate of the Autof ms1000 mass spectrometers in the identification for five types of bacteria is over 93%, the identification error is less than 3%, and the no identification rate is 0. It was indicated Autof ms1000 identification was an acceptable method.Conclusions: Autof MS 1000 mass spectrometer can be utilized to identify clinical isolates. An upgrade database is recommended to correctly identify rare strains.
The enhancing antibiofilm activity of curcumin on Streptococcus mutans strains from severe early childhood caries
Authors: Li Bingchun , Pan Ting , Lin Huancai , Zhou Yan 
Published: June 2020

Abstract
BackgroundStreptococcus mutans (S. mutans) is one of the main cariogenic bacteria for caries. It was found that the clinical strains of S. mutans isolated from caries active population have stronger cariogenic ability than the isolates from caries-free (CF) people. Previous studies have found that curcumin can inhibit biofilm formation of S. mutans UA159. The objective of this study is to explore the antibiofilm effect of curcumin on the clinical isolates of S. mutans from severe early childhood caries(SECC).ResultsThe isolates from SECC group had more biomass than CF group (t = 4.296, P < 0.001). The acidogenicity and aciduricity of the strains from two groups showed no significant difference. After treatment with curcumin, the viability of biofilm was reduced to 61.865%±7.108% in SECC and to 84.059%±10.227% in CF group at 24 h (P < 0.05). The net reduction of live bacteria and total bacteria in the SECC group was significantly higher than that of the CF group (live bacteria t = 3.305, P = 0.016; total bacteria t = 2.378, P = 0.045) at 5 min. For 24 h, the net reduction of live bacteria and total bacteria in the SECC group was significantly higher than that of the CF group (live bacteria t = 3.305, P = 0.016; total bacteria t = 2.378, P = 0.045). The reduction of biofilm thickness reduced significantly in 5 min (t = 4.110, P = 0.015) and in 24 h (t = 3.453, P = 0.014). Long-term (24 h) curcumin treatment inhibited the amount of EPS in SECC group from (25.980 ± 1.156) µm3/µm2 to (20.136 ± 1.042) µm3/µm2, the difference was statistically significant (t = 7.510, P < 0.001). The gene of gtfC, gtfD, ftf, gbpB, fruA and srtA in the CF group and the gtfB, gtfC, gtfD, ftf, gbpB, srtA in SECC group were respectively reduced after 5 min curcumin treatment. After 24 h treatment, the gtfB, gtfC, gtfD, ftf, gbpB, fruA and srtA in both two groups were downregulation, all the differences were statistically significant.ConclusionsCurcumin has antibiofilm activity on clinical strains of S. mutans, especially for those isolated from SECC.
Measurement of Sustainment of Prevention Programs and Initiatives: The Sustainment Measurement System Scale
Authors: Palinkas Lawrence A. , Chou Chih-Ping , Spear Suzanne E. , Mendon Sapna J. , Villamar Juan A. , Brown C. Hendricks 
Published: June 2020

Abstract
Background
A large knowledge gap exists regarding the measurement of sustainability of evidence-based prevention programs for mental and behavioral health. This paper describes an instrument that is a component of the Sustainment Measurement System (SMS), designed to assess both determinants and outcomes of efforts to sustain prevention programs and initiatives.
Methods
A 42-item scale was administered to 186 representatives of 145 programs funded by 7 SAMHSA prevention grant initiatives. Scale items were identified from qualitative data collected from 45 representatives of 10 programs and 8 SAMHSA program officers and organized into 9 categories of sustainment determinants and sustainment outcomes. Factor structure identified from principal axis factor analysis and elimination of items with CFA coefficients less than .300 resulted in a 35-item scale with 8 determinant factors and 1 outcome factor.
Results
Confirmatory Factor Analysis provided support for a 35-item model fit to the data. The SMSS demonstrated good inter-item reliability and all determinant factors were significantly associated with sustainment outcome individual and global measures.
Conclusions
The SMSS is easy to use and demonstrates good reliability and convergent and discriminant validity in assessing likelihood of sustainment of SAMHSA funded prevention programs and initiatives upon termination of original funding. The measure demonstrates potential in identifying predictors of program sustainment and as a tool for enhancing the likelihood of successful sustainment through ongoing evaluation and feedback.
Effect of Vitamin D supplementation and outdoor activity on serum 25-Hydroxyvitamin D among children in North China: an observational study
Authors: Zhang Xuguang , Chen Yanping , Jin Shanshan , Bi Xinxin , Chen Dongkai , Zhang Dongmei , Liu Li , Jing Hong , Na Lixin 
Published: June 2020

Abstract
Background
Living at high latitudes is one of the risk factors for vitamin D deficiency in children. However, evidence on vitamin D improvement for this pediatric population to date is limited. This study aims at evaluating the effect of different supplementation methods and outdoor activity time on the vitamin D status of children in North China.
Methods
A total of 55,925 children aged 1 month to 18 years old were recruited from pediatric outpatient departments from July 2016 to June 2017. Data on demographics, anthropometric measurements, vitamin D supplementation, and outdoor time were recorded. The serum levels of 25-hydroxycholecalciferol (25(OH)D) were determined by high performance liquid chromatography tandem–mass spectrometry. Logistic regression analysis was performed to assess the association of vitamin D supplementation or outdoor time with blood vitamin D status, adjusted for age, gender, BMI for age, and seasons.
Results
The overall rate of hypovitaminosis D was 65.60%. Of the children’s outdoor time, 35.63%, 31.95%, and 32.42% were below 30 min/d, 30–60 min/d and over 60 min/d, respectively. Furthermore, the proportion of iatrogenic supplementation, voluntary supplementation and no vitamin D supplementation among the children was 16.48%, 32.87%, and 50.65%, respectively. After adjusted for confounding factors, vitamin D supplementation was associated with a lower risk of hypovitaminosis D, with OR (95% CI) of 0.191 (0.180, 0.202) in children with iatrogenic supplementation and 0.423 (0.404, 0.443) in those with voluntary supplementation, compared with children without vitamin D supplementation. In addition, longer outdoor time was associated with a lower risk of hypovitaminosis D [0.479 (0.456, 0.504) for 60 min/d, 0.737 (0.701, 0.776) for 30–60 min/d], independent of vitamin D supplementation.
Conclusions
High prevalence of vitamin D deficiency was found in children living at high latitudes. Vitamin D supplementation and outdoor time are all negatively associated with children’s vitamin D deficiency. Routine 25(OH)D testing combined with vitamin D supplementation might be an effective approach to prevent hypovitaminosis D among children living at high latitudes.
Morbidities and mortality among infants of HIV-1-infected mothers with bacterial vaginosis in Kenya
Authors: Mwenda Ngugi , Nduati Ruth , Kosgey Mathew , Kerich Gregory 
Published: June 2020

Abstract
Background: This study aimed to assess the effects of maternal bacterial vaginosis (BV) on the morbidity and mortality of HIV-exposed infants of women enrolled in a randomized controlled trial (pre-dating antiretroviral therapy) at birth, 6 months, and 12 months. Methods: Four hundred and twenty-five HIV-positive pregnant women were enrolled in this trial and were categorized as exposed if they had a laboratory-based diagnosis of BV (Nugent method). We compared the morbidity and mortality of infants of the mothers at birth, 6 months, and 12 months. We assessed morbidities from the mother’s history and clinical examination during scheduled and non-scheduled visits. The data that were collected longitudinally were then analyzed via multiple logistic regression with the generalized estimating equation. An independent correlation structure was assumed to evaluate the specific morbidity risks to infants associated with exposure to BV. We used the Kaplan–Meier method to generate the cumulative hazard curve, to determine mortalities at different stages between the two groups. Overall, only data for 328 infants were complete and used in the analysis.Results: Data were available for 159 and 171 BV exposed and non-exposed mothers, respectively. Exposure to BV was not associated with any neonatal morbidity at birth, but was associated with adverse maternal condition (unadjusted odds ratio [OR], 2.93; 95% confidence interval [CI], 1.19–7.20, P=0.02) and maternal hospital admissions (unadjusted OR, 1.95; 95% CI, 1.08–3.51, P=0.02). At 6 months, infants of BV exposed mothers had higher odds of bloody stool (adjusted OR, 3.08; 95% CI, 1.11–10.00, P=0.04), dehydration (adjusted OR, 2.94; 95% CI, 1.44–6.37, P=0.01), vomiting (adjusted OR, 1.64; 95% CI, 1.06–2.56, P=0.03), and mouth ulcers (adjusted OR, 12.8; 95% CI, 2.27–241.21, P=0.02). At 12 months, exposure to BV was associated with dehydration (adjusted OR, 1.81; 95% CI, 1.05–3.19, P=0.03) and vomiting (adjusted OR, 1.39; 95% CI, 1.01–1.92, P=0.04). Kaplan­–Meier survival analysis showed no association of BV with infant mortality (P=0.65); however, the cumulative hazard curve showed a higher trend toward deaths among BV exposed infants.Conclusion: Our findings demonstrate that BV is a good predictor of maternal and infant morbidities. Infants of both HIV and BV exposed mothers can manifest these symptoms at any stage within a year of growth. Adverse maternal condition and hospitalization of mothers after birth could indicate exposure to BV. Bloody stool, dehydration, vomiting, and mouth ulcers could indicate exposure to BV among infants.