bluuebunny/crossref_metadata_2025_split huggingface dataset:
Real-World Experience With Acute Infusion Reactions to Ustekinumab at 2 Large Tertiary Care Centers
Authors: Spencer Elizabeth A , Kinnucan Jami , Wang Julie , Dubinsky Marla C 
Published: April 2020

AbstractBackgroundUstekinumab is approved for Crohn’s disease and Ulcerative colitis with acute infusion reactions reported at a rate of 0.9%–4.5%.MethodsA retrospective chart review was conducted on inflammatory bowel disease (IBD) patients experiencing an acute infusion reaction to ustekinumab at 2 large institutions.ResultsAcute ustekinumab infusion reactions occurred in 16 patients with Crohn’s disease (CD) and Ulcerative colitis (UC), at a rate of 0.8%–3%. Patients were all naïve to ustekinumab, receiving their initial IV induction. Ninety-three percent subsequently tolerated the injection without issues.ConclusionsIn this large, real-world study of acute infusion reactions to ustekinumab, the rate was similar to that seen in clinical trials—0.8%–3%.
Overlap matrix concentration in optimal Bayesian inference
Authors: Barbier Jean 
Published: April 2020

AbstractWe consider models of Bayesian inference of signals with vectorial components of finite dimensionality. We show that under a proper perturbation, these models are replica symmetric in the sense that the overlap matrix concentrates. The overlap matrix is the order parameter in these models and is directly related to error metrics such as minimum mean-square errors. Our proof is valid in the optimal Bayesian inference setting. This means that it relies on the assumption that the model and all its hyper-parameters are known so that the posterior distribution can be written exactly. Examples of important problems in high-dimensional inference and learning to which our results apply are low-rank tensor factorization, the committee machine neural network with a finite number of hidden neurons in the teacher–student scenario or multi-layer versions of the generalized linear model.
Hypergeometric presentation for one-loop contributing to H → Z γ
Authors: Phan Khiem Hong , Tran Dzung Tri 
Published: April 2020

Abstract
In this paper, new analytic formulas for one-loop contributing to Higgs decay channel $H \rightarrow Z\gamma$ are presented in terms of hypergeometric functions. The calculations are performed by following the technique for tensor one-loop reduction developed in [A. I. Davydychev, Phys. Lett. B 263 (1991) 107]. For the first time, one-loop form factors for the decay process are shown which are valid at arbitrary space–time dimension $d$.
ORGAN ABSORBED DOSE ESTIMATION REFLECTING SPECIFIC ORGAN MASSES WITH SIMPLE SCALING OF REFERENCE DOSES USING THE ORGAN MASSES
Authors: Manabe Kentaro , Koyama Shuji 
Published: April 2020

Abstract
Estimating organ absorbed doses in consideration of person-specific parameters is important for radiation protection in diagnostic nuclear medicine. This study proposes a straightforward method for estimating the organ dose that reflects a specific organ mass by scaling the reference organ dose using the inverse ratio of the specific organ mass to the reference organ mass. For the administration of radiopharmaceuticals labelled by 99mTc or 123I, the organ doses for the liver, spleen, red marrow and thyroid obtained by the method were compared with those generated by a Monte Carlo simulation. The discrepancies were less than 14% for the liver, spleen and thyroid. Conversely, in some cases, the red marrow discrepancies were greater than 30% due to the wide distribution of red marrow in the trunk and head regions. This study confirms that the method of scaling organ doses can be effective for estimating mass-specific doses for solid organs.
Educational needs and psychoeducation interventions in clozapine users: a narrative review
Authors: Verdoux H. , Quiles C. 
Published: April 2020

ObjectiveIncreasing users' knowledge about the expected benefits and risks of clozapine may contribute to the initiation and maintenance of clozapine in persons with treatment‐resistant schizophrenia (TRS). The objective was to identify the educational needs of clozapine users and the interventions aimed at addressing these needs.MethodWe systematically searched multiple electronic databases for articles: (i) exploring educational needs of clozapine users and of their relatives; and (ii) reporting educational interventions aimed at addressing these needs. Data were synthesized narratively.ResultsA total of 30 articles published from 1990 to 2019 in 8 countries fulfilled our inclusion criteria. As most studies on educational needs (n = 18) were carried out in persons already taking clozapine, educational needs of TRS patients who were candidates for clozapine treatment are not well documented. Users’ level of knowledge about clozapine was often poor, especially about adverse effects or interactions with other substances, with a poor retention of information delivered at treatment initiation. Among 12 studies reporting educational interventions in clozapine users, five provided quantitative outcomes. Their findings suggest that structured educational programmes may contribute to promote clozapine initiation and to improve users' knowledge about this drug.ConclusionThe literature is sparse on structured educational interventions about clozapine, and only one randomized controlled trial was identified. A promising strategy emerging from this review may be staging educational interventions according to the evolving needs of persons with TRS before clozapine use, at initiation and during maintenance treatment.
Intestinal Immune Responses in Patients Infected with EnterotoxigenicEscherichia coliand in Vaccinees
Authors: Wennerås Christine , Qadri Firdausi , Bardhan Prodeep K. , Sack R. Bradley , Svennerholm Ann-Mari 
Published: April 2020

ABSTRACTImmune responses against enterotoxigenicEscherichia coli(ETEC) were examined in Bangladeshi adults with naturally acquired disease and compared to responses in age-matched Bangladeshi volunteers who had been orally immunized with a vaccine consisting of inactivated ETEC bacteria expressing different colonization factor antigens (CFs) and the B subunit of cholera toxin. B-cell responses in duodenal biopsy samples, feces, intestinal washings, and blood were determined. Because most of the patients included in the study were infected with ETEC expressing CS5, immune responses to this CF were studied most extensively. Vaccinees and patients had comparable B-cell responses against this antigen in the duodenum: the median numbers of antibody-secreting cells (ASC) were 3,300 immunoglobulin A (IgA) ASC/107mononuclear cells (MNC) in the patient group (n= 8) and 1,200 IgA ASC/107MNC in the vaccinees (n= 13) (not a significant difference). Similarly, no statistically significant differences were seen in the levels of duodenal B cells directed against enterotoxin among vaccinees and patients. A comparison of the capacities of the various methods used to assess mucosal immune responses revealed a correlation between numbers of circulating B cells and antibody levels in saponin extracts of duodenal biopsy samples (r= 0.58;n= 13;P= 0.04) after vaccination. However, no correlation was seen between blood IgA ASC and duodenal IgA ASC after two doses of vaccine. Still, a correlation between numbers of CF-specific B cells in blood sampled from patients early during infection and numbers of duodenal B cells collected 1 week later was apparent (r= 0.70;n= 10;P= 0.03).
Effect of MALP-2, a Lipopeptide from
Mycoplasma fermentans
, on Bone Resorption In Vitro
Authors: Piec Grazyna , Mirkovitch Jelena , Palacio Silvia , Mühlradt Peter F. , Felix Rolf 
Published: April 2020

ABSTRACT

Mycoplasmas may be associated with rheumatoid arthritis in various animal hosts. In humans, mycoplasma arthritis has been recorded in association with hypogammaglobulinemia.
Mycoplasma fermentans
is one mycoplasma species considered to be involved in causing arthritis. To clarify which mycoplasmal compounds contribute to the inflammatory, bone-destructive processes in arthritis, we used a well-defined lipopeptide, 2-kDa macrophage-activating lipopeptide (MALP-2) from
M. fermentans
, as an example of a class of macrophage-activating compounds ubiquitous in mycoplasmas, to study its effects on bone resorption. MALP-2 stimulated osteoclast-mediated bone resorption in murine calvaria cultures, with a maximal effect at around 2 nM. Anti-inflammatory drugs inhibited MALP-2-mediated bone resorption by about 30%. This finding suggests that MALP-2 stimulates bone resorption partially by stimulating the formation of prostaglandins. Since interleukin-6 (IL-6) stimulates bone resorption, we investigated IL-6 production in cultured calvaria. MALP-2 stimulated the liberation of IL-6, while no tumor necrosis factor was detectable. Additionally, MALP-2 stimulated low levels of NO in calvaria cultures, an effect which was strongly increased in the presence of gamma interferon, causing an inhibition of bone resorption. MALP-2 stimulated the bone-resorbing activity of osteoclasts isolated from long bones of newborn rats and cultured on dentine slices without affecting their number. In bone marrow cultures, MALP-2 inhibited the formation of osteoclasts. It appears that MALP-2 has two opposing effects: it increases the bone resorption in bone tissue by stimulation of mature osteoclasts but inhibits the formation of new ones.
Role of Nitric Oxide in the Enhancement of Pentylenetetrazole-Induced Seizures Caused byShigella dysenteriae
Authors: Balter-Seri Judith , Yuhas Yael , Weizman Abraham , Nofech-Mozes Yehuda , Kaminsky Elizabeth , Ashkenazi Shai 
Published: April 2020

ABSTRACTConvulsions and encephalopathy are frequent complications of childhood shigellosis. We studied the role of nitric oxide (NO) inShigella-related seizures in an animal model. Pretreatment of mice withShigella dysenteriae60R sonicate elevated serum NO levels and enhanced the convulsive response to pentylenetetrazole (PTZ), as indicated by a higher mean convulsion score and a higher number of mice responding with seizures. Treatment of the mice withS-methylisothiourea sulfate (SMT), a potent inhibitor of inducible NO synthase (NOS), prevented the elevation of serum NO levels and concomitantly reduced the enhanced response to PTZ. The mean convulsion scores were 0.7, 0.7, 1.3, and 0.8 for mice treated with saline, saline and SMT,S. dysenteriae60R sonicate, andS. dysenteriae60R sonicate with SMT, respectively (P= 0.001 for 60R sonicate versus saline andP= 0.013 for 60R sonicate versus 60R sonicate with SMT). The corresponding seizure rates were 40, 44, 75, and 47% for saline, saline with SMT,S. dysenteriae60R sonicate, andS. dysenteriae60R sonicate with SMT, respectively (P= 0.0004 for 60R sonicate versus saline andP= 0.005 for 60R sonicate versus 60R sonicate with SMT). In contrast, injection ofN-nitro-l-arginine, a selective inhibitor of constitutive NOS, neither abolished the elevation of serum NO nor attenuated the enhancement of seizures. These findings indicate that NO, induced byS. dysenteriae60R sonicate, is involved in enhancing the susceptibility to seizures caused byS. dysenteriae.
Cytokine Control of the Granulomatous Response inSchistosoma mansoni-Infected Baboons: Role of Exposure and Treatment
Authors: Mola Paul W. , Farah Idle O. , Kariuki Thomas M. , Nyindo Mramba , Blanton Ronald E. , King Christopher L. 
Published: April 2020

ABSTRACTVariations in exposure and treatment may contribute to heterogeneity in immunity and granuloma-induced pathology in human schistosomiasis. To examine this hypothesis, olive baboons were either repeatedly infected withSchistosoma mansonicercariae or received an equivalent dose in a single infection. They were then cured with praziquantel and reinfected with a single exposure. Serial liver biopsies were obtained throughout the course of the experiment, and cytokine responses by peripheral blood mononuclear cells were measured every 2 to 3 weeks. Reinfection after treatment resulted in a twofold-smaller granuloma size at 6 and 9 weeks after infection compared to the size for the same period after primary infection (P< 0.001) but had no effect at 16 or 19 weeks postinfection. The pattern of exposure did not influence granuloma size. During primary infection schistosome-soluble egg antigen (SEA)-induced cytokine production correlated with granulomatous inflammation. Cytokine levels peaked during the acute infection, declined with chronic infection, and became undetectable after treatment. Reinfection after treatment stimulated a two- to three-fold increase in SEA-specific interleukin-4 (IL-4), IL-5, IL-10, IL-2, and transforming growth factor β (TGF-β) production and a marked rise in SEA-specific immunoglobulin E (IgE) and IgG regardless of the type of exposure. Cytokine production was significantly greater in repeatedly exposed animals (P< 0.001). SEA-induced gamma interferon production, however, did not increase with reinfection after treatment. SEA-induced TGF-β was the only cytokine that remained elevated as the infection become chronic and correlated with diminished hepatic granuloma size, implying its participation in down-modulation. These studies demonstrate that baboons partially retain their ability to down-modulate the granulomatous response after treatment.
circKIF4A promotes tumorogenesis of glioma by targeting miR-139-3p to activate Wnt5a signaling
Authors: Huo Long-Wei , Wang Ya-Fei , Bai Xiao-Bin , Zheng Hu-Lin , Wang Mao-De 
Published: April 2020

Abstract
Background
Glioma has the characteristics of high incidence and mortality, and is a common malignant tumor of the central nervous system. Circular RNAs (circRNAs) have been reported to play vital roles in progression of cancer including glioma, and circKIF4A is up-regulated in glioma tissues. However, its role and mechanisms in gliomas are unclear.

Methods
circKIF4A and miR-139-3p were determined by qRT-PCR. Transwell assay, wound-healing assay, cell colony formation and flow cytometry were performed to measure cell invasion, migration, proliferation and apoptosis. Western blotting was used to evaluate Wnt/β-catenin pathway-related protein. Luciferase reporter assays confirmed the relationship among circKIF4A, miR-139-3p and Wnt5a. Sphere formation was performed to measure the ability of glioma-initiating cells (GICs) spheroid formation. A nude mouse xenograft model was established and immunohistochemical staining was used to detect Ki-67 and Wnt5a levels.

Results
circKIF4A and Wnt5a were up-regulated and miR-139-3p was down-regulated in both glioma cells and tissues. circKIF4A promoted Wnt5a expression by sponging miR-139-3p. Knockdown of circKIF4A inhibited the colony formation ability, migration and invasion, and promoted the apoptosis of glioma cells by regulating miR-139-3p. Knockdown of circKIF4A inhibited Wnt/β-catenin signaling pathway and proliferation-related signal via miR-139-3p. Furthermore, knockdown of circKIF4A or overexpression of miR-139 suppressed the ability of sphere formation of GICs and inhibitd Wnt/β-catenin signaling pathway and proliferation-related signal in GICs. Additionally, depletion of circKIF4A decreased the expression level of Wnt5a and Ki-67, inhibited tumorigenesis in xenograft modes.

Conclusion
circKIF4A was overexpressed in glioma, and knockdown of circKIF4A suppressed glioma progression via miR-139-3p/Wnt5a axis. The results indicated that circKIF4A may be a potential target for clinical treatment of glioma.
IncobotulinumtoxinA for the treatment of spasticity in children with cerebral palsy – a retrospective case series focusing on dosing and tolerability
Authors: León-Valenzuela Angel , Palacios Juan Sánchez , del Pino Algarrada Rogelio 
Published: April 2020

Abstract
Background
IncobotulinumtoxinA (Xeomin®) is a botulinum neurotoxin type A with established efficacy in the treatment of upper-limb spasticity in adults. This retrospective case series in a university hospital setting aimed to elucidate the safety and tolerability of incobotulinumtoxinA for treatment of spasticity in children with cerebral palsy.

Methods
Participants received incobotulinumtoxinA injections up to a maximum total dose of 600 U, 24 U/kg body weight. Medical records were reviewed for key demographic information, incobotulinumtoxinA exposure, and adverse effects (AEs).

Results
Sixty-nine children were included (mean age [SD], 8.3 [3.9] years; 44/69 [63.8%] male). One-hundred-and-ninety-one injections were administered, with mean (SD) of 2.8 (1.5) treatment cycles/participant and dosing interval of 6.0 (1.7) months. The number of muscles injected increased from 2.4 (1.2) at cycle 1 to 4.2 (1.9) at cycle 6. The mean (SD) total incobotulinumtoxinA dose increased from 191.7 (126.2) U, (8.5 [5.4] U/kg body weight) at cycle 1 to 368.0 (170.1) U, (9.9 [5.5] U/kg body weight) at cycle 6. Seventy four adverse effects (37.5% of injections) were reported, the most frequent was injection pain (93.2% of AEs). Only three AEs were considered directly treatment-related by injectors: muscle weakness, generalized weakness, and fever.

Conclusions
Our clinical experience indicates that incobotulinumtoxinA is a well-tolerated treatment option for focal spasticity in children with cerebral palsy.

Trial registration
As the study was observational and retrospective, no EudraCT registration number was requested. The internal code assigned to the study in the administrative resolution was: 1143-N-15.
Isolation and efficacy of the endophytic fungus, Beauveria bassiana (Bals.) Vuillemin on grapevine aphid, Aphis illinoisensis Shimer (Hemiptera: Aphididae) under laboratory conditions
Authors: Sayed Samy , El-Shehawi Ahmed , Al-Otaibi Saad , El-Shazly Samir , Al-Otaibi Saqer , Ibrahim Reda , Alorabi Mohammed , Baazeem Alaa , Elseehy Mona 
Published: April 2020

AbstractThe endophytic fungus, Beauveria bassiana has been found to occur endophytically in hundreds of plant species tissues and has been inoculated for establishment in different plants. It has negative effect on piercing-sucking insects in grapevine plants. Thus, the present study aimed to detect the endophytic fungus, B. bassiana throughout ITS genes analysis in the grapevine plant, Vitis venifera cv. Taify and assess its potential for controlling the grapevine aphid, Aphis illinoisensis Shimer (Hemiptera: Aphididae). The obtained 5 isolates were DNA sequenced for ITS region. Data analysis showed that there were 2 different isolates (accession number: MN900613 for isolate Bb-Taif1 and MN900614 for isolate Bb-Taif2). The values of LC50 were 6.041 × 104, 3.199 × 105 spores/ml while those of LC90 were 6.13 × 106 and 7.474 × 107 spores/ml after 3 days of treatment by Bb-Taif1 and Bb-Taif2, respectively. These values showed that the virulence of Bb-Taif1 isolate was higher in its efficacy than the Bb-Taif2 isolate. Further investigations are needed to evaluate its efficacy on other piercing sucking or chewing insect pests throughout conidia spraying, soil inoculation, and/or root immersion for grapevine plants.
A novel tri-band reconfigurable microstrip patch antenna
Authors: Li Wen Tao , Wei Meng , Badamchi Bahareh , Subbaraman Harish , Shi Xiaowei 
Published: April 2020

Abstract
In this paper, a novel tri-band reconfigurable patch antenna with simple structure is presented. By changing the on-off state of only two PIN diodes, the antenna can operate in three bands, namely X-band, Ku-band, and Ka-band. The overall size of the antenna is 0.24λ

L
× 0.5λ

L
× 0.019λ

L
, where λ

L
is the free-space wavelength of the lowest operating frequency. A prototype is fabricated and measured to verify the design. The measurement results are in good agreement with the simulation results, which indicate that the proposed antenna can be flexibly switched between three bands of 10.9–11.18 GHz, 15.65–15.9 GHz, and 32.3–33.6 GHz with stable radiation patterns.
Proteomic analysis of synovial tissue in rheumatoid arthritis, OLFM4 promotes fibroblast like synoviocytes proliferation in RA
Authors: Ren Xiaoyu , Xu Ke , Xu Jing , Geng Manman , Lu Chao , Kong Linbo , Cai Yongsong , Hou Weikun , Lu Yufeng , Aihaiti Yirixiati , Wang Ru , Xu Peng 
Published: April 2020

Abstract
Background To understand the roles of synovial tissue in rheumatoid arthritis (RA), we comprehensively investigated the protein profiles of synovial tissues in patients with RA and osteoarthritis (OA). Methods Protein were isolated from synovial tissues of 46 patients (22 with RA, 24with OA). Proteins were digested and labeled with TMT kit. All the peptide were send to LC-MS/MS analysis, and processed with Mascot and Proteome Discovery (version 1.4) search engines. All the protein data were analysis with Gene ontology (GO) and KEGG on line database. Four up-regulated protein were examined with western blotting and IHC. The RNAi were used for revealing the OLFM4 function in FLS. Results In total, over 500 proteins were identified as the different expression protein compared RA and OA synovia tissues, including 239 proteins were up-regulated in RA, and 271 proteins were up-regulated in OA. GO and KEGG analysis of the different expressed protein mostly identified as developmental processes and protein processing in ER. RT-qPCR, western blotting and IHC confirmed that there was a strong over expressed OLFM4 in RA synovia tissues. OLFM4 could be up-regulated under inflammatory stimulation, and participate in fibroblast like synoviocytes proliferation. Conclusion patients with RA possessed considerably different protein profiles of synovial tissues from with OA. The unique protein profiles of RA synovial tissues, such as the OLFM4 and MZB1 had been up-regulated compared to the OA, may reflect the pathophysiology of RA.
Design of a GFP reporter for splicing analysis in mammalian cells
Authors: Menezes Arthur T. , Nagasse Helder Y. , Coltri Patricia Pereira 
Published: April 2020

Abstract
Objective: The great majority of eukaryotic genes are formed by exons and introns. Pre-RNA transcripts are extensively co-transcriptionally processed, with the addition of a cap group at the 5′ end, intron removal and exon ligation (splicing) followed by addition of a poly-A tail at 3′ end. Splicing is performed by specialized macromolecular machinery named spliceosome, composed of five small ribonucleoprotein particles (snRNPs) and several proteins. The activity of this complex is highly accurate due to coordinated activity of its components. Altered splicing have already been related to the development of several diseases as amyotrophic lateral sclerosis and different types of cancer. Detailed understanding of splicing regulation in eukaryotic cells can be achieved using splicing reporter systems. Results: We designed a new splicing reporter plasmid suitable for analysis in mammalian cells. Our reporter is based on splicing of the GFP sequence. The greatest advantages of this system are the ease of visualization of the splicing reaction, which needs only a fluorescence microscope, and the possibility of quantification of splicing efficiency using real-time PCR. The use of this system allows rapid and easy detection of the splicing reactions.