bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Novel Role of LINC01235 in Metastasis of Gastric Cancer Cells by Inducing Epithelial-mesenchymal Transition
Authors: Zhang Cheng , Liang Yu , Zhang Chun-Dong , Dai Dong-Qiu 
Published: August 2020

Abstract
BackgroundLncRNAs play a vital role in the tumorigenesis of gastric cancer (GC). The present study aims to explore the role of LINC01235 in clinical significance, prognostic prediction and GC metastasis.MethodsWe identified differentially expressed lncRNAs using stomach adenocarcinoma RNA-Seq data from The Cancer Genome Atlas (TCGA). The expression of LINC01235 in GC cell lines and tissues were confirmed by qRT-PCR assay. The overall survival analysis and univariate/ multivariate Cox regression analysis were performed to explore the prognostic value of LINC01235. In vitro assays were utilized to assess the effect of LINC01235 in cell migration and invasion. Western blotting measured the expression of EMT-induced proteins.ResultsThis study determined that LINC01235 expression has a higher fold changes by analyzing TCGA RNA-Seq data. qRT-PCR assay confirmed that LINC01235 is significantly over-expressed in GC cells and tissues. Additionally, the overall survival analysis showed that patients with a higher LINC01235 expression had a poorer prognosis than those with a lower LINC01235 expression. Univariate Cox regression analysis indicated that high LINC01235 expression is positively correlated with poor prognosis. Moreover, LINC01235 was an independent poor prognostic marker for GC in multivariate Cox analysis. In vitro assays suggested that LINC01235 knockdown suppresses GC cell migration and invasion. GSEA revealed that high LINC01235 expression is strongly enriched in EMT pathway. Western blotting results revealed that LINC01235 silencing decreases the expression of EMT-induced proteins.ConclusionLINC01235 could promote GC cell metastasis via EMT and function as a prognostic biomarker.
Nuclear-capture of endosomes drives depletion of nuclear G-actin to promote SRF/MRTF gene expression and cancer cell invasiveness
Authors: Marco Sergi , Neilson Matthew , Moore Madeleine , Pérez-García Arantxa , Hall Holly , Mitchell Louise , Blanco Giovanny Rodriguez , Hedley Ann , Zanivan Sara , Norman Jim 
Published: August 2020

Abstract
Signals are relayed from receptor tyrosine kinases (RTKs) at the cell surface to effector systems in the cytoplasm and nucleus, and coordination of this process is important for the execution of migratory phenotypes, such as cell scattering and invasion. The endosomal system influences how RTK signalling is coded, but the ways in which it transmits these signals to the nucleus to influence gene expression are not yet clear. Here we show that an RTK, cMET promotes Rab17-dependent endocytosis of EphA2, another RTK, followed by centripetal transport of EphA2-positive endosomes. EphA2 then mediates physical capture of endosomes on the outer surface of the nucleus; a process involving interaction between the nuclear import machinery and a nuclear localisation sequence in EphA2’s cytodomain. Nuclear capture of EphA2 promotes RhoG-dependent phosphorylation of the actin-binding protein, cofilin to oppose nuclear import of G-actin. The resulting depletion of nuclear G-actin drives transcription of Myocardin-related transcription factor (MRTF)/serum-response factor (SRF)-target genes to implement cell scattering and the invasive behaviour of cancer cells.
Methods to Evaluate the Twin Formation Energy: Comparative Studies of the Atomic Simulations and in-situ Tem Tensile Tests
Authors: Kim Hong-Kyu , Kim Sung-Hoon , Ahn Jae-Pyoung 
Published: August 2020

Abstract
Deformation twinning (DT), one of the major deformation modes in a crystalline material, has typically been analyzed using generalized planar fault energy (GPFE) curves. Despite the significance of these curves in understanding the twin nucleation and its effect on the mechanical properties of crystals, their validity has never been evaluated experimentally. In this comparative study based on the first-principles calculation, molecular dynamics (MD) simulation, and quantitative in-situ tensile testing of Al nanowires (NWs) inside a transmission electron microscopy (TEM) system, we present both a theoretical and an experimental approach that enable the measurement of a part of the twin formation energy of the perfect Al crystal. The proposed experimental method is also regarded as an indirect but quantitative means for validating the GPFE theory.
A Comprehensive Follow-Up Study Identifying Diagnostic Evidences for Secondary Finding
Authors: li shu , haoyu li , neng xiao , yixiang lin , wenxian yu , yingjing li , hui xi , zhengjun jia , fuli yu , hua wang , xiao mao 
Published: August 2020

Abstract
Background：There was a lacking of clinical diagnostic evidence in follow-up studies for reporting of secondary variants in 59 genes in American College of Medical Genetics and Genomics recommendations for reporting secondary findings and various strategies were applied to interpret the secondary variants. Results: Out of 1330 participants performed whole-exome sequencing, we identified 15 families with convincing clinical evidence. After Sanger validation and a comprehensive clinical follow-up, 10 families with both convincing clinical evidence and convincing genetic evidence of hereditary variants were found. Detailed clinical presentations and related clinical evidence were collected. Conclusions: Our research is a comprehensive follow-up study to identify secondary variants with convincing genetic and clinical evidence and it could help improve the strategy of screening actionable secondary variants and contribute to translation of genetic findings into medical practice.
Open Versus Arthroscopic Release for Lateral Patellar Compression Syndrome: A Randomized Control Trial
Authors: Hamawandi Sherwan , Amin Hazhar , Al-Humairi Ameer Kadhim 
Published: August 2020

Abstract
Background: Lateral patellar compression syndrome is one of the causes of anterior knee pain in young adults and resulted from tight lateral patellar retinaculum, which can be treated by surgical release of tight retinaculum after failure of conservative measures. Surgical release can be done by open or arthroscopic procedures. There was no randomized control trial to compare between these two procedures in considering of functional outcome and complications. The aim of our study is to compare between open and arthroscopic release of lateral patellar compression syndrome in relation of functional outcome, time of surgical procedure, length of hospital stays, intraoperative and postoperative complications as bleeding, infection, recurrence and patellar instability with 2 years of follow up. Methods: 80 patients, age (21-49 years), were divided randomly into 2 groups (A and B). Group A (40 patients) were treated with open release after diagnostic arthroscopy. Group B (40 patients) were treated by arthroscopic release. All these patients are diagnosed as lateral patellar compression syndrome depending on clinical features, MRI and diagnostic arthroscopy and they had failure of conservative measures of quadriceps strengthening and analgesics for 6 months. All patients were assessed by Lysholm knee scoring scale before surgery and at periods of 2 weeks, 6 weeks, 6 months, 12 months and 24 months after surgery.Results: There is significant difference in functional outcome, measured by Lysholm knee scoring scale, between preoperative and postoperative assessment periods in both groups (P<0.001). There is significantly better functional outcome at 2 years of follow up with the arthroscopic release (P=0.018). There is no recurrence in both groups, but there were 4 patients develop medial patellar instability in the group that was treated by open release. There are 2 patients develop hemarthrosis in arthroscopic group and one patient develop superficial wound infection in the group of open release. There is no significant difference in the postoperative complications between two groups.Conclusion: Both open and arthroscopic lateral release for patients with lateral patellar compression syndrome (without instability, limb malalignment or dysplasia) are effective surgical procedures but arthroscopic release can achieve better functional outcome than open release with less risk of development of post-operative patellar instability.Trial registration: NCT, NCT04130412. Registered 15 October 2019 -Retrospectively registered, https://www.clinicaltrials.gov/NCT04130412
Association of Diabetes and Outcomes in Patients with COVID-19: A Propensity Score Matched Analyses from a French Retrospective Cohort
Authors: Sutter Willy , Duceau Baptiste , Carlier Aurélie , Trimaille Antonin , Pommier Thibaut , Weizman Oriane , Cellier Joffrey , Geneste Laura , Panagides Vassili , Marsou Wassima , Deney Antoine , Attou Sabir , Delmotte Thomas , Ribeyrolles Sophie , Chemaly Pascale , Karsenty Clément , Giordano Gauthier , Gautier Alexandre , Chaumont Corentin , Guilleminot Pierre , Sagnard Audrey , Pastier Julie , Vignac maxime , Mika delphine , Fauvel Charles , Pezel Théo , Cohen Ariel , Bonnet Guillaume , Roussel Ronan , POTIER Louis 
Published: August 2020

Abstract
Background: To compare the clinical outcomes between patients with and without diabetes admitted to hospital with COVID-19.Methods: Retrospective multicentre cohort study from 24 academic tertiary medical centres in France including 2851 patients (675 with diabetes) hospitalised for COVID-19 between February 26 and April 20, 2020. A propensity score matching method (1:1 matching including patient characteristics, medical history, vital signs, and laboratory results) was used to compare patients with and without diabetes (n=603 in each group). The primary outcome was admission to intensive care unit (ICU) or in-hospital death. Results: Patients with diabetes were older (71 ± 13 vs. 65 ± 18 years; p<0.001), were less often female (38% vs. 44%; p<0.001) and more likely to have comorbidities: hypertension (79% vs 42%; p<0.001), coronary heart disease (23% vs 9%; p<0.001), stroke (13% vs 8%; p<0.001), heart failure (17% vs 9%; p<0.001), chronic kidney disease (26% vs 10%; p<0.001), and chronic obstructive pulmonary disease (7% vs 5%; p<0.05). The primary outcome occurred in 584 (36.4%) patients with diabetes compared to 246 (26.8%) in those without diabetes (p<0.001). After propensity score matching, the risk of primary outcome was similar in patients with and without diabetes (hazard ratio [HR] 1.16, 95%CI 0.95-1.41, p=0.14) and was 1.29 (95%CI 0.97 – 1.69) for in-hospital mortality, 1.26 (95%CI 0.93 – 1.72) for mortality without transfer in ICU, and 1.14 (95%CI 0.88 – 1.47) for transfer to ICU.Conclusions: In this retrospective cohort of patients hospitalised for COVID-19, diabetes was not significantly associated with a higher risk of COVID-19 severe outcomes after propensity score matching.Trial registration NCT04344327
LacminCC: Lightweight Anonymous Communication Model In Cloud Computing
Authors: Li Fengyin , Wang Yanli , Ju Hongwei , Yu Xinying , Wang Zhaojie , Zhou Huiyu 
Published: August 2020

Abstract
With increasing application of cloud computing and big data technologies, a large amount of personal information is stored on the Internet, which raises the issue of privacy leakage. In order to protect people's data privacy, this paper firstly presents a new anonymous Identify-Based Encryption (IBE) scheme and shows the proof of its security under the Bilinear Diffie-Hellman Security Assumption. Then, by introducing the anonymous IBE scheme into an anonymous communication model, this paper introduces a new lightweight anonymous communication model for cloud computing, which guarantees the anonymity of system users and the security of messages during the communication in small groups. Our analysis shows that, the proposed communication model cannot only reduce memory consumption and improve message transmission efficiency, but also effectively resist traffic-analysis attacks, node eavesdropping, and finally achieve secure anonymous communication in cloud computing.
Dynamic versus Static Ultra-widefield Fluorescein Angiography in Eyes with Diabetic Retinopathy: A Pilot Prospective Cross-sectional Study
Authors: Shen Hangqi , Wang Jing , Niu Tian , Chen Jili , Xu Xun 
Published: August 2020

Abstract
Background: Dynamic angiography can present additional information. However, evidence-based support in clinical application of dynamic modalities remain insufficient. The purpose of this study is to analyze differences in ultra-widefield fluorescein angiography (UWFA) findings between time-lapse dynamic and static imaging in eyes with diabetic retinopathy (DR).Methods: Twenty-eight eyes of 28 DR patients undergoing UWFA were included in the cross-sectional study. A series of UWFA images from each patient were converted into a time-lapse video in a Moving Picture Experts Group 4 movie format as a dynamic image. A single clear arteriovenous phase image was chosen as a static UWFA image. Non-perfusion index (NPI) and its correlation with intraretinal microvascular abnormality and neovascularization in different retinal zones were compared between dynamic and static UWFA imaging in severe nonproliferative diabetic retinopathy (NPDR) and proliferative diabetic retinopathy (PDR).Results: NPI appeared to increase from the center to the far-periphery in both groups. Dynamic NPI appeared to be lower in the total retinal area (0.25 vs 0.28, p=0.005), far-periphery (0.30 vs 0.34, adjusted p=0.040) and superior quadrant (0.27 vs 0.34, adjusted p=0.045) in contrast to static NPI. Far-peripheral NPI was associated with intraretinal microvascular abnormality in posterior area in both groups.Conclusion: Time-lapse dynamic UWFA imaging is a useful method in the differential diagnosis of hypofluorescence in the most peripheral region, which may provide a reliable measurement of NPI and improve the diagnostic accuracy of DR.
Sarcopenic Characteristics of Active Older Adults: A Cross-sectional Exploration.
Authors: Huschtscha Zoya , Parr Alexander , Porter Judi , Costa Ricardo da 
Published: August 2020

Abstract
Background: Ageing is associated with a decline in skeletal muscle mass and function (strength and power), known as sarcopenia. Inadequate dietary protein and inactivity have been shown to accelerate sarcopenia outcomes, occurring at different rates in males and females. Regardless, active older adults who often exceed the exercise guidelines still show signs of sarcopenia. This study aimed to explore the link between age, physical activity, protein intake, and biological sex with skeletal muscle mass, strength, power, and physical capacity/performance in active older adults. Methods: Fifty-four active older adults were grouped according to age (middle aged: 50-59 years, and older age: ≥60 years), exercise volume (low: <150min/week, moderate: >150-299min/week, and high: >300min/week), protein intake (low: <0.8g/kg body mass (BM), moderate: ≥0.8-1.19g/kgBM, and high: ≥1.2g/kgBM), and biological sex (males and females). Skeletal muscle and fat mass (dual x-ray absorptiometry), strength (1-repetition maximum using leg press, chest press, lateral pull down, and hand grip), power (counter movement jump), and general fitness (cardiorespiratory capacity and gait speed) were assessed. Data were grouped based on variables, and a one-way ANOVA (or non-parametric equivalent) was applied to assess group differences. Results: The middle-aged group had a 13%, 17%, and 12% higher leg press, chest press, and lateral pull down, respectively, compared to the older aged group (P<0.05). Participants that reported moderate and high training volumes had lower body fat mass compared to those that reported lower training volumes (26.1%, 25.1%, and 35.6%, respectively; P<0.001). Similarly, higher leg press (22% and 27%) and chest press (22% and 23%) was observed with moderate and high training volumes compared with lower. Higher protein intakes were associated with significantly less body fat mass (P=0.019), higher leg strength (P=0.038) and relative power (W/kg) (P=0.048) compared to the moderate and low protein intake groups. Significant differences based on biological sex were observed for all outcomes expect for gait speed (P=0.611) and cardiorespiratory fitness (P=0.147).Conclusion: Contributions of age, physical activity, daily protein intake, and biological sex can explain the individual variation in outcomes related to changes in body composition, strength, power, and/or cardiorespiratory fitness in a cohort of active older adults.
AAV9-Retro Mediates Efficient Transduction with Axon Terminal Absorption and Blood-brain Barrier Transportation
Authors: Lin Kunzhang , Zhong Xin , Li Lei , Ying Min , Yang Tian , Zhang Zhijian , He Xiaobin , Xu Fuqiang 
Published: August 2020

Abstract
Recombinant adeno-associated viruses (rAAVs), especially which permit efficient gene transfer to neurons from axonal terminals or across the blood-brain barrier, are useful vehicles for the structural and functional studies of neural circuit, and for the treatment of many gene-deficient brain diseases that needs to compensate for the correct genes to every cell in the whole brain. However, AAVs with these two advantages have not been reported. Here, we describe a new capsid engineering method, which draws on advantage combination of different capsids, and aims to yield a capsid that can provide more alternative routes of administration, which are more suitable for wide-scale transduction of the CNS. A new AAV variant, AAV9-Retro, was developed by inserting the 10-mer peptide fragment from AAV2-Retro into the capsid of AAV9, and the biodistribution properties were evaluated in mice. By intracranial and intravenous injection in the mice, we found that AAV9-Retro can retrogradely infect projection neurons with efficiency comparable to AAV2-Retro, and retains the characteristic of AAV9 that can transport across the nervous system. Our strategy provides a new tool for the manipulation of neural circuits, and for the future preclinical and clinical treatment of some neurological and neurodegenerative disorders.
MELD-Albumin score predicts 30-day mortality in high-risk patients with acute pulmonary embolism admitted to intensive care units
Authors: İdin Kadir , Dereli Seçkin , Yenerçağ Mustafa , Kaya Ahmet 
Published: August 2020

Abstract
Background: The Model for End-stage Liver Disease excluding the international normalised ratio (INR, MELD-XI) and modified MELD, which uses albumin in place of the INR (MELD-Albumin) scores reflect liver and renal function and are predictors of mortality. However, their prognostic value in acute pulmonary embolism (APE) has not been studied.Methods: We assessed the predictive value of the MELD scores in patients diagnosed with high-risk APE admitted to the intensive care unit (ICU). The primary outcome was 30-day mortality.Results: Of the 273 patients included in the study, 231 were survivors and 42 were non-survivors. The mortality rate was 15.3%. The mean Meld-XI and MELD-Albumin scores were significantly higher in the non-survivors than in the survivors (MELD XI, 11.8 ± 1.8 and 10.6 ± 1.43, respectively; p = 0.002; MELD-Albumin, 10.5 ± 1.6 and 8.7 ± 1.1, respectively; p = 0.001). The multiple logistic regression analysis identified the MELD-XI (hazard ratio [HR]: 3.029, confidence interval [CI]: 1.06–1.21, p = 0.007) and MELD-Albumin (HR: 1.13, CI: 1.06–1.21, p = 0.002) scores as independent predictors of mortality. Receiver operating characteristic analysis revealed that the predictive power of the MELD-Albumin score (0.871 ± 0.014; p < 0.001) was higher than those of the MELD-XI (0.726 ± 0.022, p < 0.001), APACHE III (0.682 ± 0.024, p < 0.001), and PESI (0.624 ± 0.023, p < 0.001) scores.Conclusions: The MELD-Albumin score is an easily calculable, reliable, and practical risk assessment tool and independent predictor of 30-day mortality in patients with high-risk APE.
Mortality rate among critically ill patients with COVID-19 in a medical system with adequate hospital resources: a prospective observational study
Authors: Routsi Christina , Magira Eleni , Kokkoris Stelios , Siembos Ilias , Vrettou Charikleia , Zervakis Dimitris , Ischaki Eleni , Malahias Sotiris , Sigala Ioanna , Asimakos Andreas , Daidou Theodora , Kaltsas Panagiotis , Douka Evangelia , Sotiriou Adamandia , Markaki Vassiliki , Temberikidis Prodromos , Koroneos Apostolos , Politis Panagiotis , Mastora Zafiria , Dima Efrosini , Tsoutsouras Theodoros , Papahatzakis Ioannis , Gioni Panagiota , Strilakou Athina , Maraguti Aikaterini , Mizi Eleftheria , Kanavou Ageliki , Sarri Aikaterini , Gavrielatou Evdokia , Mentzelopoulos Spyros , Kalomenidis Ioannis , Papastamopoulos Vassilios , Kotanidou Anastasia , Zakynthinos Spyros G 
Published: August 2020

Abstract
Background: For critically ill patients with coronavirus disease 2019 (COVID-19) who require intensive care unit (ICU) admission, mortality rates vary widely depending on many factors, among which hospital resources and clinical setting seem important. We sought to determine the outcome of critically ill patients admitted in the usual multidisciplinary ICUs of a big referral for COVID-19 tertiary-care hospital with adequate resources.Methods: We performed a prospective observational study of all adult patients with COVID-19 consecutively admitted to four COVID-designated ICUs at Evangelismos Hospital, Athens, Greece, from March 11 to April 27, 2020.Results: Among 50 critically ill patients, ICU and hospital mortality for the entire cohort was 32% (16/50), whereas 66% (33/50) of patients were discharged alive from the ICU and 2% (1/50) were still treated in the ICU until June 16, 2020. ICU and hospital mortality for those who received invasive mechanical ventilation was 39% (16/41). Patients who eventually died had already increased risk of death on ICU admission, as suggested by the high values of the Acute Physiology and Chronic Health Evaluation II and Sequential Organ Failure Assessment scores, the presence of current malignancy and occurrence of cardiac arrest in 44% (7/16) of patients, and the general need for circulatory support by noradrenaline. Median PaO2/FiO2 on ICU admission for the entire cohort was 121 mmHg [interquartile range (IQR), 86-171 mmHg] and most patients had moderate and severe acute respiratory distress syndrome (ARDS) according to the Berlin Definition. The primary cause of death of all patients was multi-organ failure, most commonly due to sepsis, whereas none died from refractory hypoxemia, neurologic dysfunction or withdrawal of life support. Hospital stay was long in patients who survived [median 24 days (IQR, 15-35 days)] and was frequently complicated by bacteremias [36% (12/33)].Conclusion: Severely ill COVID-19 patients with moderate and severe ARDS may have equal or even lower mortality rates compared to ARDS due to other causes, when they are admitted in general ICUs with experienced and adequate staff without limitations in hospital resources, where established ARDS therapies are used.