bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nanomolar Melatonin Influences Insulin Synthesis and Secretion in Rat Insulinoma INS-1E Cells
Authors: Jung Eui-Man , Joo Seong-Soo , Yoo Yeong-Min 
Published: October 2020

Abstract
Background: Pancreatic beta-cell dysfunction results in reductions of insulin synthesis/secretion, cell survival, and insulin sensitivity thereby inducing diabetes mellitus. In this study, how nanomolar melatonin regulates insulin synthesis and secretion in rat insulinoma INS-1E cells was investigated. Methods: Western Blot and ELISA were performed to investigate the insulin synthesis and secretion at melatonin concentrations of 10-100 nM for 48 h and 10-500 nM for 72 h in rat insulinoma INS-1E cells.Results: Melatonin significantly increased the insulin protein level in INS-1E cells above the level in control cells without melatonin or glucose treatments and decreased the insulin protein level in media with glucose. At melatonin concentrations of 10-100 nM for 48 h and 10-500 nM for 72 h, increases and decreases occurred in dose-dependent manners. Luzindole or 4-phenyl-2-propionamidotetralin (4P-PDOT), melatonin receptor (MR) antagonists, inhibited the melatonin-induced insulin protein level in cells and media. Levels of membrane vesicle trafficking-related proteins including Rab5, GOPC, and phospho-caveolin-1 proteins significantly increased with melatonin treatment above that in control cells without melatonin or glucose treatments, whereas expressions of EEA1, clathrin, APPL1, and syntaxin-6 proteins significantly decreased with melatonin treatment. The increases in the phosphorylation of the mammalian target of rapamycin (p-mTOR) and Raptor protein levels (mTOR Complex 1 (mTORC1)) were consistent with the increments in the expressions of p-Akt (Ser473, Thr308). Also, expression levels of Bcl-2 and Bcl-xL proteins were significantly increased compared to those in control cells without melatonin or glucose treatments, whereas the Bax protein level decreased. Luzindole or 4P-PDOT decreased the melatonin-induced levels of p-mTOR (Ser2448), Raptor, p-Akt (Ser473), and Bcl-2 proteins, whereas MR antagonists increased the melatonin-suppressed level of Bax proteins. Conclusion: These results indicate that nanomolar melatonin regulates insulin synthesis and secretion associated with membrane vesicle trafficking-related proteins, including Rab5, GOPC, p-Caveolin-1, EEA1, and clathrin through the Akt/mTORC1 pathway.
Patients’ Perspectives on the Feasibility, Acceptability, and Impact of a Community Health Worker Program: A Qualitative Study
Authors: Chang Wei , Oo May , Rojas Adriana , Damian April Joy 
Published: October 2020

Abstract
Background: Growing evidence suggests that community health workers (CHWs) can play a key role in delivering culturally competent preventive care services, addressing social determinants of health (SDOH), and improving the health outcomes of underserved populations. This study sought to understand the experiences of patients with Type 2 diabetes working with a CHW in a safety net setting.Methods: A convenience sample (n=13) who participated in a six-month CHW pilot program at a federally qualified health center (FQHC) was recruited for in-person or telephone semi-structured interviews that included 17 open-ended questions regarding patients’ experiences with the CHW program. Interview transcripts were analyzed using a traditional text analysis method based on a grounded theory approach to identify themes related to the participants’ perspectives of the CHW program.Results: This study highlights the CHW program can improve satisfaction in accessing health services and community resources, and overall health outcomes of patients in the safety net practices. Moreover, patients discussed the positive impact of the CHW program in terms of improved medical knowledge, access to medical services, and self-efficacy. The CHW’s professionalism also helped to establish rapport and trust that further supported behavior changes related to patients’ health goals.Conclusion: Patients’ overall positive perception regarding the feasibility, acceptability, and impact of the CHW program indicated that the intervention may be a viable solution to address the health and social needs of patients in safety net settings. Additionally, CHW programs can help to bridge the gap between patients and healthcare team by mitigating barriers to care.
WITHDRAWN: Understanding the complexity of population health interventions: assessing intervention system theory (ISyT)
Authors: Cambon Linda , ALLA François 
Published: October 2020

Abstract
BackgroundA better understanding of what is happening inside the ”black box” of population health interventions is needed because of their inherent complexity. The theory-driven intervention/evaluation paradigm is one approach used for this purpose. However, barriers related to semantic or practical issues stand in the way of its complete integration into evaluation designs.Methods and discussionIn this study, we aimed to clarify how various theories, models, and frameworks could contribute to conceiving a grounded theory, called interventional system theory (ISyT), suitable for understanding the black box of population health interventions and acknowledging their complexity. We suggest that this interventional system theory (ISyT) could guide evaluation processes, whatever evaluation design is applied.ConclusionWe believe that such clarification could contribute to encouraging the use of theories in complex intervention evaluations, and to identifying ways to consider the transferability and the scalability of interventions.
Assessment of Body Composition in Obese Pediatric Patients: a Method Comparison
Authors: Thajer Alexandra , Skacel Gabriele , Truschner Katharina , Jorda Anselm , Vasek Martin , Horsak Brian , Strempfl Johanna , Kautzky-Willer Alexandra , Kainberger Franz , Greber-Platzer Susanne 
Published: October 2020

Abstract
BackgroundThe determination of body composition is an important method to investigate obese patients and to evaluate the efficacy of medical interventions. Bioelectrical impedance-based methods are convenient, non-invasive and widely applied for this purpose, but need to be validated for their use in young obese patients.MethodsWe compiled data from three independent studies on different aspects of obesity in children and adolescents, measuring body composition with two bioelectrical impedance-based devices (TANITA and BIA). Further, for a small patient group additional data were collected with air displacement plethysmography (BOD POD) and DXA.ResultsBoth, the combined data of the entire study population of 123 patients (age: 6-18 years, BMI: 21-59 kg/m²) and the data of each individual study, showed that TANITA and BIA yield significantly different results on body composition, with TANITA overestimating body fat percentage and fat mass relative to BIA and underestimating fat-free mass (p < 0.001 for all three parameters). A Bland-Altman plot revealed that both methods show little agreement and produce clinically relevant differences for all three parameters. In addition, we detected gender-specific differences with both methods, body fat percentage being significantly lower (p < 0.01) and fat-free mass significantly higher (p < 0.001) in males than females. A comparison of bioelectrical impedance-based methods with BOD POD and DXA on a small patient group indicated no significant difference between methods.ConclusionsBoth bioelectrical impedance-based methods provide significantly different results on body composition in young obese patients and the data thus cannot be used interchangeably. Routine clinical practice may nonetheless use these devices but must adhere to a specific device for repetitive measurements to ascertain comparability of data.Trial registration: Study#2, Children`s KNEEs study, ClinicalTrials NCT02545764. Registered 10 September 2015, https://clinicaltrials.gov/ct2/show/results/NCT02545764
Biceps Tenodesis Combined With Rotator Cuff Repair Increases Functional Status And Elbow Strength In Both Operated And Contralateral Arms
Authors: KILIC Ali Ihsan , HAPA Onur , OZMANEVRA Ramadan , DEMIRKIRAN Nihat Demirhan , GURSAN Onur 
Published: October 2020

Abstract
Purpose: Aim of the present study was to prospectively evaluate the elbow flexion and supination strengths, and the functional outcomes of patients after arthroscopic rotator cuff repair combined with simultaneous biceps tenodesis.Methods: Nineteen patients who underwent arthroscopic rotator cuff repair and biceps tenodesis with at least 24 months follow-up were included. Patients were evaluated using a visual analogue scale (VAS) for bicipital groove pain, American Shoulder and Elbow Surgeons (ASES), and constant scores(CS), biceps apex distance (BAD), elbow flexion and supination strengths.Results: VAS for biceps groove measurement averages of postoperative 6th, 12th and 24th month were lower in comparison to pre-operative data and were considered to be statistically significant (p<0.05). Constant score, an average of all post-operative measurements and scores were found higher than pre-operative values and was considered to be statistically significant (p<0,01). There was a significant difference in the comparison of operated and contralateral forearm supination and elbow flexion muscle strength measurements at postoperative 3rd and 6th-month follow-up (p<0.01).Conclusion: Arthroscopic biceps tenodesis into the anchors of lateral row combined rotator cuff repair provides an increase in strength of elbow flexion and forearm supination, while decreases pain. Level of Evidence: Level IV
A Novel Anatomical Locking Plate Combined with an Anterior Column Screw and Magic Screw for Acetabular Fractures Involving Both Columns: Reduction Effect and Functional Outcomes
Authors: Zhang Gongzi , Zhang Shuwei , Sui Yi , Wang Xiang , Su Xiuyun , Cao Zuo , Luo Shijie , Tang Peifu , Zhang Lihai 
Published: October 2020

Abstract
Background: A novel anatomical locking plate (NALP) was designed. The NALP is fixed via an anterior column screw and a Magic screw to apply pressure on the acetabular fracture ends. This study was performed to compare the reduction effect and clinical outcome of the NALP versus the anatomical locking plate (ALP) for acetabular fractures involving both columns.Methods: From January 2013 to January 2018, 22 patients with acetabular fractures involving both columns were treated using the NALP or ALP in a single institution. The general condition and type of fracture were recorded. The NALP and ALP groups were compared regarding the Letournel fracture classification, operative approach, time from injury to operation, intraoperative bleeding volume, and operation time. The radiographic reduction outcome and hip function at final follow-up were compared between the two groups based on the Matta scores and Harris hip scores, respectively.Results: The NALP was used in 10 patients, while the ALP was used in 12. Three patients had transverse fractures, 12 had transverse fractures involving posterior wall, and 7 had double-column fractures. The two groups had no significantly differ regarding sex, age, affected side, Letournel fracture classification, operative approach, or follow-up duration. The time from injury to the operation was 20.70±1.2 days in the NALP group and 13.17±7.5 days in the ALP group (p=0.089). The operation time was 332.20±128.9 minutes in the NALP group and 257.50±91.6 minutes in the ALP group (p=0.128). The intraoperative blood loss volume was 1550.00±869.5 ml in the NALP group and 666.67±370.1 ml in the ALP group (p=0.011). The Matta score at final follow-up was 1.10±0.8 in the NALP group and 2.46±1.9 in the ALP group (p=0.045). The Harris hip score at final follow-up was 87.50±10.5 in the NALP group and 81.00±10.1 in the ALP group (p=0.0782).Conclusion: The NALP achieves adequate compression of the acetabular fracture ends and obtains stronger fixation than the ALP.Trial registration: ChiCTR,ChiCTR2000030825. Registered 15 March 2020 - Retrospectively registered, http://www.chictr.org.cn/ChiCTR2000030825
Evaluation of Anticancer Property of Claviceps Purpurea Secondary Metabolites on Different Cancer Cell Lines
Authors: ST Lokesh , Basaiah Thippeswamy , HV Sowmya , ML Vijaya Kumar 
Published: October 2020

Abstract
The present study deals with the secondary metabolites of Claviceps purpurea extract, which has been confirmed as an empirically potential cure for cancer. The purpose of this research was to prove scientifically the anticancer potential of ethyl acetate extract as its effects on the cell cycle, membrane apoptosis, DNA fragmentation and nuclear staining of lung cancer (A549) cell lines. The MTT method was used to measure cytotoxic effects, ethyl acetate extract of Claviceps purpurea showed IC50 value of 56.38 µg/ml compare to other cancer cell lines like breast cancer (MCF-7) cell lines (90.04 µg/ml) and cervical cancer (HeLa) cell lines (84.97 µg/ml) and normal cell lines fibroblast (3T3L1) does not show IC50 values due to lesser percentage of inhibition. The effect of inhibition of cell cycle and induction of cell apoptosis was measured by the flow cytometry method. DNA fragmentation studied by the gel electrophoresis method. The results showed, the ethyl acetate extract of Claviceps purpurea gives an induction of apoptosis effect on A549 cancer cell through inhibition of cell cycle in the G0-G1, Synthesis, and G2/M phases. In necrotic cell regions apoptosis was found. In DNA fragmentation 80 μg/ml concentration of extract showed significant results against A549 cell lines. In the nuclear staining study, membrane blebbing will be found. It indicates the extract was very active against cancer cell lines and it occurs in late apoptotic phase. In this study of all parameters, Colchicine was used as standard for cell cycle studies, for membrane apoptosis studies vinblastine and DNA fragmentation studies hydrogen peroxide was used.
WITHDRAWN: SiC/nano α-Al2O3 Double-Layer Coating on Graphite Component via Pack Cementation and Electrophoretic Deposition
Authors: Ghazvini Ali Asghar Sadeghi , Shaker Majid , Ebadzadeh Touraj , Raissi Babak 
Published: October 2020

Abstract
Graphite and carbonaceous parts are being extensively employed in industrial and high-tech fields. Since these materials suffer from severe oxidation at elevated temperatures, it is vital to keep them safe from being damaged and corroded at high-temperature working conditions. In this study, a double-layer coating was prepared to achieve this aim. Primarily, SiC was coated on graphite samples via pack cementation method and then a layer of nano α-Al2O3 was coated on it through the electrophoretic deposition (EPD) method to improve the oxidation resistance at high temperatures. This alumina coating was further sintered only for 20 minutes at a relatively low sintering temperature of 1350 ºC in a microwave furnace to densify and close the oxygen permeation paths. Finally, this double-layer fabricated coating could reduce the weight loss by oxidation about 50 times in comparison with the bare graphite. Regarding the excellent oxidation protection performance of this double-layer coating and its feasibility for production scale-up, it can be further investigated to protect various carbon-based engineering materials from oxidation at high temperatures.
Combination of β2-AR Blocker and COX-2 Inhibitor Suppresses Development of Oral Squamous Cell Carcinoma
Authors: Zhang Chong , Ma Zhen , Liao Xianxiang , Liu Shiqi , Fang Fang , Tao Shichen , Liu Xiaotong , Wen Xutao , Chen Xingjin , Chen Meiyu , Ning Xuansheng , Mai Huaming 
Published: October 2020

Abstract
Background To study the effect of inhibiting progression of oral squamous cell carcinoma (OSCC) through blocking β2-adrenergic receptor (β2-AR) and inhibiting Cyclooxygenase-2 (COX-2). Methods Transwell invasion assay and wound-healing assay were used to investigate whether invasion and metastasis of OSCC cells (SCC9, Cal27 cell lines and primary OSCC cells) would be inhibited after treated by β2-AR blocker (ICI118,551), COX-2 inhibitor (Etodolac) or both. Real time quantitative polymerase chain reaction (RT-qPCR), Western blot and enzyme linked immunosorbent assay (ELISA) were used to detect expression change of genes related to invasion and metastasis in OSCC cells after drug treatment. In vivo, 6-8 weeks old female Balb/c nude mice were randomly divided into control group, ICI118,551 treatment group, etodolac treatment group and combined treatment group. Cal27 cells were used to establish OSCC xenograft models. The survival days, tumor sizes and lymph nodes metastasis were compared among these groups. Immunohistochemistry, Western blot and ELISA were carried out to detect expression change of invasion and metastasis relative genes. Results ICI118,551 or etodolac could inhibit invasion and metastasis of OSCC cells, and the inhibition of combined treatment was more significant. Moreover, RT-qPCR and Western blot showed that epidermal growth factor receptor (EGFR), transforming growth factor-β1 (TGF-β1), interleukin-1β (IL-1β), matrix metalloproteinase 2 (MMP2) were downregulated after treatment, especially in combined treatment group. In vivo, the combined treatment group could significantly prolong survival days and inhibit tumor size. Immunohistochemistry, Western blot and ELISA showed the expression of above-mentioned genes were also downregulated. Conclusions Invasion and metastasis of OSCC can be inhibited through blocking β2-AR and inhibiting COX-2, it can be a potential treatment of OSCC.
PCDH9 target RAC1/ROS-NADPH-oxidase complex with the assistance of Pyk2 interaction through Cyclin D1 trafficking
Authors: Zhang Jiaojiao , Yang Hui-Zhi , Liu Shuang , Islam Md Obaidul , Zhu Yue , Chen RongYi , Wang Zuhua 
Published: October 2020

Abstract
Background: Melanoma has dramatically increased during last 30 years with low five-year survival and prognosis rate.Methods: Melanoma cells (A375 and G361) were chosen as in vitro model. The interference and overexpression of PCDH9 were infected by lentivirus. The effects of PCDH9 on melanoma cells were assessed in terms of alteration of PCDH9 such as cell viability, apoptosis, cell cycle and wound-healing assay. Moreover, expressions of PCDH9 with other genes (MMP2, MMP9, Pyk2 Cyclin D1, RAC1 and FAK) were also assessed by PCR and western blotting, immunohistochemical analysis. The protein complex immunoprecipitation was operated for protein-protein interaction (PPI) of PCDH9. Originlab 2020 was used for statistical analysis.Results: Melanoma cells treated with PCDH9 showed influence melanoma as similar function as Hace1 that regulate RAC1/ROS-dependent NADPH oxidase complexes through targeting complex-bound RAC1 with the help of Pyk2, while RAC1 directly influence RAC1/ROS-NADPH-oxidase complex to induce metalloproteinases (MMPs), that are involved in cell migration and proliferation. Moreover, PCDH9 could influence the expressions of MMP2, MMP9, Pyk2, Cyclin D1 and RAC1, except FAK, decreased cell viability, cell migration and increased apoptosis, but did not exert significant effects on cell cycle. Besides, the immunohistochemical results exhibited lower PCDH9 expression in malignant melanoma samples than benign naevus tissue or/and normal skin, and PCDH9 was expressed in the cytoplasm rather than nuclei. The results of immunoprecipitation demonstrated that PCDH9 bind with RAC1 and Pyk2, suggesting the regulation of PCDH9 to melanoma progression. Conclusion: Our results suggest that PCDH9 and RAC1 could predict for potential biomarkers prognosis of malignant melanoma, interestingly, with Pyk2’s assistance like a bridge PCDH9 can modulate melanoma progression by interacting with RAC1.
Advancing model calibration and uncertainty analysis of SWAT models using cloud computing infrastructure: LCC-SWAT
Authors: Zamani Masood , Shrestha Narayan Kumar , Akhtar Taimoor , Boston Trevor , Daggupati Prasad 
Published: October 2020

AbstractCalibration and uncertainty analysis of a complex, over-parameterized environmental model such as the Soil and Water Assessment Tool (SWAT) requires thousands of simulation runs and multiple calibration iterations. A parallel calibration system is thus desired that can be deployed on cloud-based architectures for reducing calibration runtime. This paper presents a cloud-based calibration and uncertainty analysis system called LCC-SWAT that is designed for SWAT models. Two optimization techniques, sequential uncertainty fitting (SUFI-2) and dynamically dimensioned search (DDS), have been implemented in LCC-SWAT. Moreover, the cloud-based system has been deployed on the Southern Ontario Smart Computing Innovation Platform's (SOSCIP) Cloud Analytics platform for diagnostic assessment of parallel calibration runtime on both single-node and multi-node CPU architectures. Unlike other calibrations/uncertainty analysis systems developed on the cloud, this system is capable of generating a comprehensive set of statistical information automatically, which facilitates broader analyses of the performance of the SWAT models. Experimental results on SWAT models of different complexities showed that LCC-SWAT can reduce runtime significantly. The runtime reduction is more pronounced for more complex and computationally intensive models. However, the reported runtime efficiency is significantly higher for single node systems. Comparative experiments with DDS and SUFI-2 show that parallel DDS outperforms parallel SUFI-2 in terms of both parameter identifiability and reducing uncertainty in model simulations. LCC-SWAT is a flexible calibration system and other optimization algorithms and asynchronous parallelization strategies can be added to it in future.
Novel Coronavirus Disease (COVID-19): A Narrative Review Based on Current Status
Authors: Das Rina , Mehta Dinesh K. , Dhanawat Meenakshi 
Published: October 2020

:
A novel virus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), appeared
and expanded globally by the end of the year 2019 from Wuhan, China, causing the severe
acute respiratory syndrome. During its initial stage, the disease was called the novel coronavirus
(2019-nCoV). It was named COVID-19 by the World Health Organization (WHO) on 11th February,
2020. The WHO declared the SARS-CoV-2 virus a pandemic in March 2020. On 30th January,
2020, the first case of Corona Virus Disease 2019 (COVID-19) was reported in India. Currently,
the virus has spread to almost every part of the province and the rest of the globe.

:
On the basis of novel published evidence, we efficiently summarized the reported work with reference
to COVID-19 epidemiology, pathogen, clinical symptoms, treatment, and prevention. Several
worldwide electronic scientific databases such as Pubmed, Medline, Embase, Science Direct, Scopus,
etc., were utilized for extensive investigation of relevant literature.

:
This review is written in the hope of encouraging people with the key learning points from the underway
efforts to perceive and manage SARS-CoV-2, suggesting salient points for expanding future
research.