bluuebunny/crossref_metadata_2025_split huggingface dataset:
Synthetic biology based construction of biological activity-related library of fungal decalin-containing diterpenoid pyrones
Authors: Tsukada Kento , Shinki Shono , Kaneko Akiho , Murakami Kazuma , Irie Kazuhiro , Murai Masatoshi , Miyoshi Hideto , Dan Shingo , Kawaji Kumi , Hayashi Hironori , Kodama Eiichi N. , Hori Aki , Salim Emil , Kuraishi Takayuki , Hirata Naoya , Kanda Yasunari , Asai Teigo 
Published: April 2020

AbstractA synthetic biology method based on heterologous biosynthesis coupled with genome mining is a promising approach for increasing the opportunities to rationally access natural product with novel structures and biological activities through total biosynthesis and combinatorial biosynthesis. Here, we demonstrate the advantage of the synthetic biology method to explore biological activity-related chemical space through the comprehensive heterologous biosynthesis of fungal decalin-containing diterpenoid pyrones (DDPs). Genome mining reveals putative DDP biosynthetic gene clusters distributed in five fungal genera. In addition, we design extended DDP pathways by combinatorial biosynthesis. In total, ten DDP pathways, including five native pathways, four extended pathways and one shunt pathway, are heterologously reconstituted in a genetically tractable heterologous host, Aspergillus oryzae, resulting in the production of 22 DDPs, including 15 new analogues. We also demonstrate the advantage of expanding the diversity of DDPs to probe various bioactive molecules through a wide range of biological evaluations.
Sex-associated molecular differences for cancer immunotherapy
Authors: Ye Youqiong , Jing Ying , Li Liang , Mills Gordon B. , Diao Lixia , Liu Hong , Han Leng 
Published: April 2020

AbstractImmune checkpoint blockade therapies have extended patient survival across multiple cancer lineages, but there is a heated debate on whether cancer immunotherapy efficacy is different between male and female patients. We summarize the existing meta-analysis to show inconsistent conclusions for whether gender is associated with the immunotherapy response. We analyze molecular profiling from ICB-treated patients to identify molecular differences for immunotherapy responsiveness. We perform comprehensive analyses for patients from The Cancer Genome Atlas (TCGA) and reveal divergent patterns for sex bias in immune features across multiple cancer types. We further validate our observations in multiple independent data sets. Considering that the majority of clinical trials are in melanoma and lung cancer, meta-analyses that pool multiple cancer types have limitations to discern whether cancer immunotherapy efficacy is different between male and female patients. Future studies should include omics profiling to investigate sex-associated molecular differences in immunotherapy.
Turning charge-density waves into Cooper pairs
Authors: Chikina Alla , Fedorov Alexander , Bhoi Dilipkumar , Voroshnin Vladimir , Haubold Erik , Kushnirenko Yevhen , Kim Kee Hoon , Borisenko Sergey 
Published: April 2020

AbstractThe relationship between charge-density waves (CDWs) and superconductivity is a long-standing debate. Often observed as neighbors in phase diagrams, it is still unclear whether they cooperate, compete, or simply coexist. Using angle-resolved photoemission spectroscopy, we demonstrate here that by tuning the energy position of the van Hove singularity in Pd-doped 2H-TaSe2, one is able to suppress CDW and enhance superconductivity by more than an order of magnitude. We argue that it is particular fermiology of the material that is responsible for each phenomenon, thus explaining their persistent proximity as phases.
Rapid dose-dependent Natural Killer (NK) cell modulation and cytokine responses following human rVSV-ZEBOV Ebolavirus vaccination
Authors: Pejoski David , de Rham Casimir , Martinez-Murillo Paola , Santoro Francesco , Auderset Floriane , Medaglini Donata , Pozzi Gianni , Vono Maria , Lambert Paul-Henri , Huttner Angela , Haks Mariëlle C. , Ottenhoff Tom H. M. , Villard Jean , Siegrist Claire-Anne ,   the VEBCON Consortium, Addo Marylyn M. , Agnandji Selidji Todagbe , Becker Stephan , Bejon Philip , Brosnahan Jessica S. , Fast Patricia , Huttner Angela , Krähling Verena , Kieny Marie-Paule , Kremsner Peter G. , Krishna Sanjeev , Lapujade Olivier , Moorthy Vasee , Njuguna Patricia , Savarese Barbara , Siegrist Claire-Anne ,   VSV-EBOVAC Consortium, Agnandji Selidji Todagbe , Ahmed Rafi , Anderson Jenna , Auderset Floriane , Bejon Philip , Borgianni Luisa , Brosnahan Jessica S. , Ciabattini Annalisa , Engler Olivier , Haks Mariëlle C. , Harandi Ali M. , Heppner Donald Gray , Gerlini Alice , Huttner Angela , Kremsner Peter G. , Martinez-Murillo Paola , Medaglini Donata , Monath Thomas , Ndungu Francis , Njuguna Patricia , Ottenhoff Tom H. M. , Page Mark , Pejoski David , Pozzi Gianni , Santoro Francesco , Siegrist Claire-Anne ,   VSV-EBOPLUS Consortium, Agnandji Selidji Todagbe , Anderson Jenna , Auderset Floriane , Borgianni Luisa , Ciabattini Annalisa , Dubey Sheri , Engler Olivier , Fernandes José F. , Haks Mariëlle C. , Harandi Ali M. , Gerlini Alice , Huttner Angela , Kremsner Peter G. , Martinez-Murillo Paola , Medaglini Donata , Monath Thomas , Nakaya Helder , O’Rourke Fiona , Ottenhoff Tom H. M. , Pejoski David , Pozzi Gianni , Rothenberger Sylvia , Santoro Francesco , Siegrist Claire-Anne 
Published: April 2020

AbstractThe rVSV-ZEBOV Ebolavirus vaccine confers protection within days after immunization, suggesting the contribution of innate immune responses. We report modulation of rVSV-ZEBOV vaccinee blood CD56+ NK cell numbers, NKG2D or NKp30 surface receptor expression, Killer Immunoglobulin-like Receptor (KIR)+ cell percentages and NK-cell-related genes on day 1 post immunization. Inverse correlations existed between the concentration of several plasma cytokines and inhibitory KIR+ CD56dim or cytokine-responsive CD56bright NK cells. Thus, NK cells may contribute to the early protective efficacy of rVSV-ZEBOV in humans.
A fine-tuned global distribution dataset of marine forests
Authors: Assis Jorge , Fragkopoulou Eliza , Frade Duarte , Neiva João , Oliveira André , Abecasis David , Faugeron Sylvain , Serrão Ester A. 
Published: April 2020

AbstractSpecies distribution records are a prerequisite to follow climate-induced range shifts across space and time. However, synthesizing information from various sources such as peer-reviewed literature, herbaria, digital repositories and citizen science initiatives is not only costly and time consuming, but also challenging, as data may contain thematic and taxonomic errors and generally lack standardized formats. We address this gap for important marine ecosystem-structuring species of large brown algae and seagrasses. We gathered distribution records from various sources and provide a fine-tuned dataset with ~2.8 million dereplicated records, taxonomically standardized for 682 species, and considering important physiological and biogeographical traits. Specifically, a flagging system was implemented to signal potentially incorrect records reported on land, in regions with limiting light conditions for photosynthesis, and outside the known distribution of species, as inferred from the most recent published literature. We document the procedure and provide a dataset in tabular format based on Darwin Core Standard (DwC), alongside with a set of functions in R language for data management and visualization.
A dataset of multi-functional ecological traits of Brazilian bees
Authors: Borges Rafael Cabral , Padovani Kleber , Imperatriz-Fonseca Vera Lucia , Giannini Tereza Cristina 
Published: April 2020

AbstractWorldwide, bees are the most important group of animal pollinators. The ecosystem service they provide is vital in natural areas and croplands, and the taxonomic and functional diversity associated with bees is vital in understanding ecosystem functioning ensuring biodiversity conservation, food security and human livelihoods. A dataset of bees from mountainous areas of Carajás National Forest (eastern Amazon) and Nova Lima (Atlantic Forest) is presented here. It is a compilation of sampling efforts from 1983 to 2018 through the accession of data stored in museum collections. In total, 222 and 144 bee species were recorded in Carajás and Nova Lima, respectively. This represents the most robust dataset of Brazilian bees including species traits (body size, flight range, distribution, crop pollination, sociality and nesting) of 328 species. This dataset contributes to advances in the knowledge of the functional trait ecology of wild bees and can benefit further studies that analyze the response of wild bees to land use and climate changes, and its effects on the provision of crop pollination services.
Elicitation of integrated immunity in mice by a novel pneumococcal polysaccharide vaccine conjugated with HBV surface antigen
Authors: Qian Wen , Huang Zhen , Chen Yuqiu , Yang Jinling , Wang Lili , Wu Kai , Chen Min , Chen Nanping , Duan Yongzhong , Shi Jing , Zhang Ying , Li Qihan 
Published: April 2020

AbstractThe conjugation of polysaccharides with an effective carrier protein is critical for the development of effective bacterial polysaccharide vaccines. Therefore, the identification and optimization of carrier proteins to induce an effective immune response is necessary for developing a combined vaccine. In the current study, we utilized hepatitis B virus surface antigen (HBsAg) as a novel carrier protein combined with a capsular polysaccharide molecule to develop a new pneumococcal conjugated vaccine. The specific antibodies and T cell immune response against the capsular polysaccharide and HBsAg in the mice immunized with this conjugated vaccine were evaluated. In addition, the unique gene profiles of immune cells induced by this conjugated vaccine in the immunized mice were analyzed. Our results demonstrated that the vaccine consisting of pneumonia type 33 F capsular polysaccharide (Pn33Fps) conjugated with HBsAg can induce strong specific immune responses against both antigens in vivo in immunized mice. Furthermore, the conjugated vaccine induced higher expression of genes related to the activation of immunity and higher antibody titers against Pn33Fps and HBsAg in mice than those obtained via vaccination with a single antigen. Analyses of the dynamic expression changes in immunity-related genes in mice immunized with Pn33Fps_HBs, Pn33Fps, or HBsAg indicated the potent immunogenicity of the conjugated vaccine. In addition, a pathological evaluation of the organs from immunized mice further suggested that the conjugated vaccine is safe. Together, these results indicate that a conjugated vaccine consisting of Pn33Fps with HBsAg is a novel and effective vaccine.
Fabrication of Perfusable Vascular Channels and Capillaries in 3D Liver-like Tissue
Authors: Mori Nobuhito , Akagi Yuka , Imai Yukiko , Takayama Yuzo , Kida Yasuyuki S. 
Published: April 2020

AbstractAlthough various production methods for 3D vascularised tissues have been developed, constructing capillary-like structures branching from perfusable large channels remains difficult. This study describes a method to fabricate tube-shaped 3D liver-like tissue (tubular liver tissue) with large channels and capillary-like structures using a perfusion device. The perfusion device functions as an interface between the tissue and an external pump, as it has connectors equipped with anchors that hold the tissue in response to its shrinkage, which is accompanied by the self-organisation of capillary-like structures. Histological analysis revealed that perfusion via the large channel induced capillary formation around the channel and maintained proper tissue functions. Accompanied by structural examinations, global gene expression analysis supported this finding; specifically, genes involved in angiogenesis were enriched in the perfused condition. Furthermore, we confirmed the penetrability of the capillary-like structures by infusing India ink, as well as substance exchange by measuring the amounts of secreted albumin. These lines of evidence indicate that our method can be used to construct 3D tissues, which is useful for fields of in vitro tissue regeneration for drug development and regenerative medicine.
Colloidal Silicon Dioxide in Tablet form (Carbowhite) Efficacy in Patients with Acute Diarrhea: Results of Randomized, Double-Blind, Placebo-Controlled, Multi-Center Study
Authors: Tieroshyn Vadim , Moroz Larisa , Prishliak Oleksandra , Shostakovich-Koretska Lyudmila , Kruglova Oksana , Gordienko Lyudmila 
Published: April 2020

AbstractThe acute diarrhea is a wide-spread disease. The prescription of enterosorbents is appropriate as a primary measure for the treatment of the acute diarrhea for effective prevention of the fluid and electrolyte loss, as well as method for symptom relief of the attack of the disease. Aim of the study - the antidiarrheal efficacy and safety study of high-dispersion silicon dioxide enterosorbent in tablet dosage form in patients with acute diarrhea. This was randomized, double-blind, placebo-controlled, 4-center study. Acute diarrhea was defined as three and more episodes of watery stool per day either during 48 hours or less before study entry in the patients having normal stool recently. It has been postulated that symptoms and signs of acute diarrhea have to be caused by direct infection of the gastrointestinal tract and did not associated with moderate-to-severe systemic states. 144 patients with established acute diarrhea were randomized into treatment group (enterosorbent “Carbowhite”, n = 120) or placebo group. Date collection including severity diarrhea, systemic symptoms was performed at baseline and daily during 7 days. Stool examination and serological assay were performed at baseline. The primary end points were declared as time to complete recovery from acute diarrhea. It has been found that the use of the siliceous enterosorbent (“Carbowhite”) allowed to reduce (p < 0.001) the treatment period averagely for 0.9 days (95% confidence interval 0.5–1.2 days) in comparison with placebo. Data of safety monitoring has revealed that both patient groups had negative stool culture, while initiation of antibiotic treatment was run more frequently in placebo group (8.3%) compared to investigational product group (4.1%, P = 0.044). The siliceous enterosorbent “Carbowhite” was well tolerated and reduced the recovery time of the acute episode of the diarrhea in the clinically significant form.
Urbanisation alters ecological interactions: Ant mutualists increase and specialist insect predators decrease on an urban gradient
Authors: Rocha Elise A. , Fellowes Mark D. E. 
Published: April 2020

AbstractThe modification of habitats in urban areas is thought to alter patterns of species interactions, by filtering specialist species and those at higher trophic levels. However, empirical studies addressing these hypotheses remain limited in scope and number. This work investigates (1) how main urban land uses affect predator-prey and mutualistic interactions, and (2) how specialist and generalist predators respond to size and availability of urban green spaces. In a large town in the UK, experimental colonies of ant-attended Black bean aphid Aphis fabae and non-ant-attended Pea aphid Acyrthosiphon pisum were monitored over two years. Ants were more frequently found in highly urbanised sites; however mutualistic ants were also more often encountered when the habitat was more plant diverse. Aphids were not affected by urban land uses, but A. fabae numbers were positively related to the presence of mutualists, and so indirectly affected by urbanisation. Predators were the only group negatively affected by increased urbanisation, and specialist species were positively related to increased proportion of urban green areas within the habitats. While this work supports the hypothesis that specialist predators are negatively affected by urbanisation, we also show that a fundamental ecological interaction, mutualism, is affected by urbanisation.
HgCdTe mid-Infrared photo response enhanced by monolithically integrated meta-lenses
Authors: Li Fangzhe , Deng Jie , Zhou Jing , Chu Zeshi , Yu Yu , Dai Xu , Guo Huijun , Chen Lu , Guo Shangkun , Lan Mengke , Chen Xiaoshuang 
Published: April 2020

AbstractPolarization-independent dielectric meta-lens is proposed to monolithically integrate with a HgCdTe infrared photodetector to concentrate power flux into a reduced photosensitive area for performance enhancement. Although a reduction in photosensitive area could suppress the dark current, the more seriously reduced light absorptance would degrade the specific detectivity D*. The integration of the meta-lens could reverse the situation by improving the absorptance of the photosensitive region. The meta-lens composed of an array of nano-pillars with varying diameters is formed by carving the CdZnTe substrate of the HgCdTe detector so that the integration could be accomplished in situ. The meta-lens focuses the incident light through the CdZnTe medium and at the HgCdTe photosensitive region. The focal spot is about the wavelength size and the focusing efficiency is above 63%. Concerning a HgCdTe detector with a pitch size of 40 μm × 40 μm, when the photosensitive area is reduced to 5 μm × 5 μm, the meta-lens could still keep the light absorptance above 50%, which is 49 times higher than that of the device without the meta-lens. The dark current reduces with the decreasing photosensitive area in a linear manner. When the photosensitive area shrinks from 40 μm × 40 μm to 10 μm × 10 μm or 5 μm × 5 μm, the dark current reduces by 16 or even 64 times. Compared to the pristine device, the employment of the meta-lens together with the reduction in photosensitive area could enhance D* by 5.5 times for the photosensitive area as 5 μm × 5 μm. Further, the meta-lens exhibits a good dispersion tolerance over the wavelength range from 3.3 μm to 5 μm. The averaged detectivity enhancement over this spectrum range is around 3 times for the photosensitive area as 5 μm × 5 μm. The angular response of the meta-lens integrated detector depends on the focal length. For a focal length of 73 µm or 38 µm, the angle of view for a 5 μm × 5 μm photosensitive area is 4.0° or 7.7°. For the inter-pillar distance to be 2 µm in our design, the influence of the coupling effect between the nano-pillars on the performance of the meta-lens is little.
Serum biomarkers from cell-based assays for AhRL and MIS strongly predicted the future development of diabetes in a large community-based prospective study in Korea
Authors: Lee Hong Kyu , Park Wook Ha , Kang Young Cheol , Kang Sora , Im Suyeol , Park Sol , Kim Jin Taek , Lee Minhyeok , Seok Junhee , Oh Man-Suk , Choi Hoon Sung , Pak Youngmi Kim 
Published: April 2020

AbstractExposure to environment-polluting chemicals (EPC) is associated with the development of diabetes. Many EPCs exert toxic effects via aryl hydrocarbon receptor (AhR) and/or mitochondrial inhibition. Here we investigated if the levels of human exposure to a mixture of EPC and/or mitochondrial inhibitors could predict the development of diabetes in a prospective study, the Korean Genome and Epidemiological Study (KoGES). We analysed AhR ligands (AhRL) and mitochondria-inhibiting substances (MIS) in serum samples (n = 1,537), collected during the 2008 Ansung KoGES survey with a 4-year-follow-up. Serum AhRL, determined by the AhR-dependent luciferase reporter assay, represents the contamination level of AhR ligand mixture in serum. Serum levels of MIS, analysed indirectly by MIS-ATP or MIS-ROS, are the serum MIS-induced mitochondria inhibiting effects on ATP content or reactive oxygen species (ROS) production in the cultured cells. Among 919 normal subjects at baseline, 7.1% developed impaired glucose tolerance (IGT) and 1.6% diabetes after 4 years. At the baseline, diabetic and IGT sera displayed higher AhRL and MIS than normal sera, which correlated with indices of insulin resistance. When the subjects were classified according to ROC cut-off values, fully adjusted relative risks of diabetes development within 4 years were 7.60 (95% CI, 4.23–13.64), 4.27 (95% CI, 2.38–7.64), and 21.11 (95% CI, 8.46–52.67) for AhRL ≥ 2.70 pM, MIS-ATP ≤ 88.1%, and both, respectively. Gender analysis revealed that male subjects with AhRL ≥ 2.70 pM or MIS-ATP ≤ 88.1% showed higher risk than female subjects. High serum levels of AhRL and/or MIS strongly predict the future development of diabetes, suggesting that the accumulation of AhR ligands and/or mitochondrial inhibitors in body may play an important role in the pathogenesis of diabetes.
Sulfur-enriched leonardite and humic acid soil amendments enhance tolerance to drought and phosphorus deficiency stress in maize (Zea mays L.)
Authors: Kaya Cengiz , Şenbayram Mehmet , Akram Nudrat Aisha , Ashraf Muhammed , Alyemeni Mohammed Nasser , Ahmad Parvaiz 
Published: April 2020

AbstractSoil amendments are known to promote several plant growth parameters. In many agro-ecosystems, water scarcity and drought induced phosphorus deficiency limits crop yield significantly. Considering the climate change scenario, drought and related stress factors will be even more severe endangering the global food security. Therefore, two parallel field trials were conducted to examine at what extent soil amendment of leonardite and humic acid would affect drought and phosphorus tolerance of maize. The treatments were: control (C: 100% A pan and 125 kg P ha−1), P deficiency (phosphorus stress (PS): 62.5 kg P ha−1), water deficit stress (water stress (WS): 67% A pan), and PS + WS (67% A pan and 62.5 kg P ha−1). Three organic amendments were (i) no amendment, (ii) 625 kg S + 750 kg leonardite ha−1 and (iii) 1250 kg S + 37.5 kg humic acid ha−1) tested on stress treatments. Drought and P deficiency reduced plant biomass, grain yield, chlorophyll content, Fv/Fm, RWC and antioxidant activity (superoxide dismutase, peroxidase, and catalase), but increased electrolyte leakage and leaf H2O2 in maize plants. The combined stress of drought and P deficiency decreased further related plant traits. Humic acid and leonardite enhanced leaf P and yield in maize plants under PS. A significant increase in related parameters was observed with humic acid and leonardite under WS. The largest increase in yield and plant traits in relation to humic acid and leonardite application was observed under combined stress situation. The use of sulfur-enriched amendments can be used effectively to maintain yield of maize crop in water limited calcareous soils.