bluuebunny/crossref_metadata_2025_split huggingface dataset:
Longitudinal Changes of NF-κB Downstream Mediators and Peritoneal Transport Characteristics in Incident Peritoneal Dialysis Patients
Authors: Fung Winston Wing-Shing , Poon Peter Yam-Kau , Ng Jack Kit-Chung , Kwong Vickie Wai-Ki , Pang Wing-Fai , Kwan Bonnie Ching-Ha , Cheng Phyllis Mei-Shan , Li Philip Kam-Tao , Szeto Cheuk-Chun 
Published: April 2020

AbstractThe role of intra-peritoneal mediators in the regulation peritoneal transport is not completely understood. We investigate the relation between longitudinal changes in dialysis effluent level of nuclear factor kappa-B (NF-κB) downstream mediators and the change in peritoneal transport over 1 year. We studied 46 incident PD patients. Their peritoneal transport characteristics were determined after starting PD and then one year later. Concomitant dialysis effluent levels of interleukin-6 (IL-6), cyclo-oxygenase-2 (COX-2) and hepatocyte growth factor (HGF) are determined. There were significant correlations between baseline and one-year dialysis effluent IL-6 and COX-2 levels with the corresponding dialysate-to-plasma creatinine level at 4 hours (D/P4) and mass transfer area coefficient of creatinine (MTAC). After one year, patients who had peritonitis had higher dialysis effluent IL-6 (26.6 ± 17.4 vs 15.1 ± 12.3 pg/ml, p = 0.037) and COX-2 levels (4.97 ± 6.25 vs 1.60 ± 1.53 ng/ml, p = 0.007) than those without peritonitis, and the number of peritonitis episode significantly correlated with the IL-6 and COX-2 levels after one year. In contrast, dialysis effluent HGF level did not correlate with peritoneal transport. There was no difference in any mediator level between patients receiving conventional and low glucose degradation product solutions. Dialysis effluent IL-6 and COX-2 levels correlate with the concomitant D/P4 and MTAC of creatinine. IL-6 and COX-2 may contribute to the short-term regulation of peritoneal transport.
Potential role of endoplasmic reticulum stress is involved in the protection of fish oil on neonatal rats with necrotizing enterocolitis
Authors: Zhu Xiaoli , Cui Ningxun , Yu Lingling , Cheng Ping , Cui Mingling , Zhu Xueping , Wang Jian 
Published: April 2020

AbstractNeonatal necrotizing enterocolitis (NEC) is a serious gastrointestinal disease with high death rate in premature infants. Fish oil (FO) and its constituents have been shown to ameliorate intestinal inflammation and mucosal damage. However, the underlying mechanism of action is not known. In the present study, we divided Sprague-Dawley rats into three groups: control group, NEC model group, and FO pre-feeding+NEC model group. Briefly, one week before NEC modeling, in addition to being fed with milk, the FO pre-feeding+NEC modeling group was fed with FO, the NEC group was fed with saline, and the control group was only inserted a gastric-tube for 7 days. Subsequently, histological assay, Western blot, and ELISA were performed. Pretreatment with FO attenuated the NEC symptoms, alleviated intestinal pathological injury, and decreased the expressions of pro-inflammatory cytokines such as interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α). Furthermore, pretreatment with FO reduced the expressions of endoplasmic reticulum stress (ERS) related proteins, caspase-12, and glucose-regulated protein 78 (GRP78). In addition, intestinal histopathological scores showed a significant positive correlation with intestinal expressions of IL-6, TNF-α, and caspase-12. Collectively, these results indicate that ERS pathway might be involved in the effect of FO in alleviating intestinal mucosal inflammation and injury in rats with NEC
Genome-wide association mapping and accuracy of predictions for amoebic gill disease in Atlantic salmon (Salmo salar)
Authors: Aslam Muhammad L. , Boison Solomon A. , Lillehammer Marie , Norris Ashie , Gjerde Bjarne 
Published: April 2020

AbstractAmoebic gill disease (AGD) is a parasitic disease caused by the amoebaParamoeba perurans, which colonizes the gill tissues and causes distress for the host. AGD can cause high morbidity and mortalities in salmonid and non-salmonid fish species. To understand the genetic basis of AGD and improve health status of farmed A. salmon, a population of ~ 6,100 individuals belonging to 150 full-sib families was monitored for development of AGD in the sea of Ireland. The population was followed for two rounds of AGD infections, and fish were gill scored to identify severity of disease in first (N = 3,663) and the second (N = 3,511) infection with freshwater treatment after the first gill-scoring. A subset of this gill-scored population (N = 1,141) from 119 full-sib families were genotyped with 57,184 SNPs using custom-made Affymetrix SNP-chip. GWAS analyses were performed which resulted in five significantly associated SNP variants distributed over chromosome 1, 2 and 5. Three candidate genes;c4, tnxbandslc44a4were found within QTL region of chromosome 2. Thetnxbandc4genes are known to be a part of innate immune system, and may play a role in resistance to AGD. The gain in prediction accuracy obtained by involving genomic information was 9–17% higher than using traditional pedigree information.
Resveratrol attenuates hypoxia-induced neuronal cell death, inflammation and mitochondrial oxidative stress by modulation of TRPM2 channel
Authors: Akyuva Yener , Nazıroğlu Mustafa 
Published: April 2020

AbstractHypoxia (HYPX) induced-overload Ca2+ entry results in increase of mitochondrial oxidative stress, inflammation and apoptosis in several neurons. Ca2+ permeable TRPM2 channel was gated by ADP-ribose (ADPR) and reactive oxygen species (ROS), although its activity was modulated in HYPX-exposed neurons by resveratrol (RSV). The aim of this study was to evaluate if a therapy of RSV can modulate the effect of HYPX in the TRPM2 expressing SH-SY5Y neuronal and HEK293 (no expression of TRPM2) cell lines. The SH-SY5Y and HEK293 cells were divided into four groups as control, RSV (50 μM and 24 hours), and HYPX and RSV + HYPX. For induction of HYPX in the cells, CoCl2 (200 μM and 24 hours) incubation was used. HYPX-induced intracellular Ca2+ responses to TRPM2 activation were increased in the SH-SY5Y cells but not in the HEK293 cells from coming H2O2 and ADPR. RSV treatment improved intracellular Ca2+ responses, mitochondrial function, suppressed the generation of cytokine (IL-1β and TNF-α), cytosolic and mitochondrial ROS in the SH-SY5Y cells. Intracellular free Zn2+, apoptosis, cell death, PARP-1, TRPM2 expression, caspase −3 and −9 levels are increased through activating TRPM2 in the SH-SY5Y cells exposed to the HYPX. However, the values were decreased in the cells by RSV and TRPM2 blockers (ACA and 2-APB). In SH-SY5Y neuronal cells exposed to HYPX conditions, the neuroprotective effects of RSV were shown to be exerted via modulation of oxidative stress, inflammation, apoptosis and death through modulation of TRPM2 channel. RSV could be used as an effective agent in the treatment of neurodegeneration exposure to HYPX.
Paradigm shift in aerosol chemical composition over regions downwind of China
Authors: Uno Itsushi , Wang Zhe , Itahashi Syuichi , Yumimoto Keiya , Yamamura Yuki , Yoshino Ayako , Takami Akinori , Hayasaki Masamitsu , Kim Byung-Gon 
Published: April 2020

AbstractA rapid decrease in PM2.5 concentrations in China has been observed in response to the enactment of strong emission control policies. From 2012 to 2017, total emissions of SO2 and NOx from China decreased by approximately 63% and 24%, respectively. Simultaneously, decreases in the PM2.5 concentration in Japan have been observed since 2014, and the proportion of stations that satisfy the PM2.5 environmental standard (daily, 35 µg/m3; annual average, 15 µg/m3) increased from 37.8% in fiscal year (FY) 2014 (April 2014 to March 2015) to 89.9% in FY 2017. However, the quantitative relationship between the PM2.5 improvement in China and the PM2.5 concentration in downwind regions is not well understood. Here, we (1) quantitatively evaluate the impacts of Chinese environmental improvements on downwind areas using source/receptor analysis with a chemical transport model, and (2) show that these rapid emissions reductions improved PM2.5 concentrations both in China and its downwind regions, but the difference between SO2 and NOx reduction rates led to greater production of nitrates (e.g., NH4NO3) due to a chemical imbalance in the ammonia–nitric acid–sulfuric acid–water system. Observations from a clean remote island in western Japan and numerical modeling confirmed this paradigm shift.
Functional near-infrared-spectroscopy-based measurement of changes in cortical activity in macaques during post-infarct recovery of manual dexterity
Authors: Kato Junpei , Yamada Toru , Kawaguchi Hiroshi , Matsuda Keiji , Higo Noriyuki 
Published: April 2020

AbstractBecause compensatory changes in brain activity underlie functional recovery after brain damage, monitoring of these changes will help to improve rehabilitation effectiveness. Functional near-infrared spectroscopy (fNIRS) has the potential to measure brain activity in freely moving subjects. We recently established a macaque model of internal capsule infarcts and an fNIRS system for use in the monkey brain. Here, we used these systems to study motor recovery in two macaques, for which focal infarcts of different sizes were induced in the posterior limb of the internal capsule. Immediately after the injection, flaccid paralysis was observed in the hand contralateral to the injected hemisphere. Thereafter, dexterous hand movements gradually recovered over months. After movement recovery, task-evoked hemodynamic responses increased in the ventral premotor cortex (PMv). The response in the PMv of the infarcted (i.e., ipsilesional) hemisphere increased in the monkey that had received less damage. In contrast, the PMv of the non-infarcted (contralesional) hemisphere was recruited in the monkey with more damage. A pharmacological inactivation experiment with muscimol suggested the involvement of these areas in dexterous hand movements during recovery. These results indicate that fNIRS can be used to evaluate brain activity changes crucial for functional recovery after brain damage.
Prognostic value of sarcopenia in patients with colorectal liver metastases undergoing hepatic resection
Authors: Liu Yueh-Wei , Lu Chien-Chang , Chang Ching-Di , Lee Ko-Chao , Chen Hong Hwa , Yeh Wen Shuo , Hu Wang-Hseng , Tsai Kai-Lung , Yeh Cheng-Hsi , Wee Sin-Yong , Yin Shin-Min , Wang Chih-Chi , Hung Chao-Hung 
Published: April 2020

AbstractThe prognostic significance of sarcopenia has been widely studied in different cancer patients. This study aimed to analyze the influence of sarcopenia on long-term survival in patients with colorectal liver metastasis (CRLM) undergoing hepatic resection. A retrospective analysis of 182 patients undergoing hepatic resection for CRLM was performed. Sarcopenia was determinedusing the Hounsfield unit average calculation (HUAC), a measure of muscle quality-muscledensity at preoperative abdominal computed tomography scans. Sarcopenia was defined as an HUAC score of less than 22 HU calculated using receiver operating characteristic analysis. The prognostic relevance of clinical variables and overall survival (OS) and recurrence-free survival (RFS) was evaluated. Patients with sarcopenia were older (p < 0.001) and had higher prevalence of diabetics (p = 0.004), higher body mass index (BMI) (p < 0.001) and neutrophil-to-lymphocyte ratio (p = 0.026) compared to those without. Sarcopenia was not significantly associated with OS and RFS. Multivariate Cox’s regression analysis showed that multinodularity (>3) (hazard ratio (HR) 2.736; 95% confidence interval (CI), 1.631–4.589; p < 0.001), high CEA level (≥20 ng/ml) (HR 1.793; 95% CI, 1.092–2.945; p = 0.021) and blood loss (≥300 cc) (HR1.793; 95% CI, 1.084–2.964; p = 0.023) were independent factors associated with OS. In subgroup analyses, sarcopenia was a significant factor of poor OS in the patients with multinodularity by univariate (p = 0.002) and multivariate analyses(HR 3.571; 95% CI, 1.508–8.403; p = 0.004). Multinodularity (>3) (HR 1.750; 95% CI, 1.066–2.872; p = 0.027), high aspartate aminotransferase level (HR 1.024; 95% CI, 1.003–1.046; p = 0.025) and male gender (HR 1.688; 95% CI, 1.036–2.748; p = 0.035) were independent factors of RFS. In conclusion, despite no significance in whole cohort, sarcopenia was predictive of worse OS in patients with multiple CRLM after partial hepatectomy.
Training instance segmentation neural network with synthetic datasets for crop seed phenotyping
Authors: Toda Yosuke , Okura Fumio , Ito Jun , Okada Satoshi , Kinoshita Toshinori , Tsuji Hiroyuki , Saisho Daisuke 
Published: April 2020

AbstractIn order to train the neural network for plant phenotyping, a sufficient amount of training data must be prepared, which requires time-consuming manual data annotation process that often becomes the limiting step. Here, we show that an instance segmentation neural network aimed to phenotype the barley seed morphology of various cultivars, can be sufficiently trained purely by a synthetically generated dataset. Our attempt is based on the concept of domain randomization, where a large amount of image is generated by randomly orienting the seed object to a virtual canvas. The trained model showed 96% recall and 95% average Precision against the real-world test dataset. We show that our approach is effective also for various crops including rice, lettuce, oat, and wheat. Constructing and utilizing such synthetic data can be a powerful method to alleviate human labor costs for deploying deep learning-based analysis in the agricultural domain.
The first copper(ii) complex with 1,10-phenanthroline and salubrinal with interesting biochemical properties
Authors: Masuri Sebastiano , Cadoni Enzo , Cabiddu Maria Grazia , Isaia Francesco , Demuru Maria Giovanna , Moráň Lukáš , Buček David , Vaňhara Petr , Havel Josef , Pivetta Tiziana 
Published: April 2020

Abstract
The novel copper complex [Cu(phen)2(salubrinal)](ClO4)2 (C0SAL) has been synthesised and characterised. Copper(ii) is coordinated by salubrinal through the thionic group, as shown by the UV-Vis, IR, ESI-MS and tandem mass results, together with the theoretical calculations. The formed complex showed a DPPH radical scavenging ability higher than that of salubrinal alone. Studies on lipid oxidation inhibition showed that the C0SAL concentration, required to inhibit the enzyme, was lower than that of salubrinal. The inhibition of the enzyme could take place via allosteric modulation, as suggested by docking calculations. C0SAL showed a good cytotoxic activity on A2780 cells, 82 fold higher than that of the precursor salubrinal and 1.4 fold higher than that of [Cu(phen)2(H2O)](ClO4)2. Treatment with C0SAL in SKOV3 ovarian cancer cells induced expression of GRP-78 and DDIT3 regulators of ER-stress response. The cytotoxic effect of C0SAL was reverted in the presence of TUDCA, suggesting that C0SAL induces cell death through ER-stress. In A2780 cells treated with C0SAL γ-H2AX was accumulated, suggesting that DNA damage was also involved.
Characterization of the Fe metalloproteome of a ubiquitous marine heterotroph,Pseudoalteromonas(BB2-AT2): multiple bacterioferritin copies enable significant Fe storage
Authors: Mazzotta Michael G , McIlvin Matthew R , Saito Mak A 
Published: April 2020

AbstractFe is a critical nutrient to the marine biological pump, which is the process that exports photosynthetically fixed carbon in the upper ocean to the deep ocean. Fe limitation controls photosynthetic activity in major regions of the oceans, and the subsequent degradation of exported photosynthetic material is facilitated particularly by marine heterotrophic bacteria. Despite their importance in the carbon cycle and the scarcity of Fe in seawater, the Fe requirements, storage and cytosolic utilization of these marine heterotrophs has been less studied. Here, we characterized the Fe metallome of Pseudoalteromonas (BB2-AT2). We found that with two copies of bacterioferritin (Bfr), Pseudoalteromonas possesses substantial capacity for luxury uptake of Fe. Fe : C in the whole cell metallome was estimated (assuming C : P stoichiometry ∼51 : 1) to be between ∼83 μmol : mol Fe : C, ∼11 fold higher than prior marine bacteria surveys. Under these replete conditions, other major cytosolic Fe-associated proteins were observed including superoxide dismutase (SodA; with other metal SOD isoforms absent under Fe replete conditions) and catalase (KatG) involved in reactive oxygen stress mitigation and aconitase (AcnB), succinate dehydrogenase (FrdB) and cytochromes (QcrA and Cyt1) involved in respiration. With the aid of singular value decomposition (SVD), we were able to computationally attribute peaks within the metallome to specific metalloprotein contributors. A putative Fe complex TonB transporter associated with the closely related Alteromonas bacterium was found to be abundant within the Pacific Ocean mesopelagic environment. Despite the extreme scarcity of Fe in seawater, the marine heterotroph Pseudoalteromonas has expansive Fe storage capacity and utilization strategies, implying that within detritus and sinking particles environments, there is significant opportunity for Fe acquisition. Together these results imply an evolved dedication of marine Pseudoalteromonas to maintaining an Fe metalloproteome, likely due to its dependence on Fe-based respiratory metabolism.
Cross-Sectional Trajectories of Mental State Language Development in Children With Down Syndrome
Authors: Channell Marie Moore 
Published: April 2020

PurposeThis article (a) examined the cross-sectional trajectories of mental state language use in children with Down syndrome (DS) and (b) identified developmental factors associated with its use.MethodForty children with DS aged 6–11 years generated stories from a wordless picture book and completed an assessment battery of other linguistic, cognitive, and social–emotional skills. Their narratives were coded for mental state language density (the proportion of utterances containing mental state references) and diversity (the number of different mental state terms used).ResultsThe emergence of mental state language use during narrative storytelling was observed across the sample; 0%–24% of children's utterances included references to mental states, and a variety of mental state terms were produced. Cross-sectional developmental trajectory analysis revealed that expressive vocabulary and morphosyntax were significantly related to increased mental state language density and diversity. Nonverbal emotion knowledge was significantly related to greater diversity of mental state terms used. Age and nonverbal cognition were not significant factors.ConclusionsThis first in-depth, within-syndrome characterization of mental state language use by school-age children with DS provides an important next step for understanding mental state and narrative development in this population. By identifying skills associated with the development of mental state language, this study provides an avenue for future longitudinal research to determine causal relationships, ultimately informing intervention efforts.
Broad-band spectral energy distribution of the X-ray transient Swift J1745−26 from outburst to quiescence
Authors: Chaty Sylvain , Fortin Francis , López-Oramas Alicia 
Published: April 2020

Aims. We aim to analyse our study of the X-ray transient Swift J1745−26, using observations obtained from its outburst in September 2012, up to its decay towards quiescence in March 2013.
Methods. We obtained optical and infrared observations, through override programme at ESO/VLT with FORS2 and ISAAC instruments, and added archival optical (VLT/VIRCAM), radio and X-ray (Swift) observations, to build the light curve and the broad-band spectral energy distribution (SED) of Swift J1745−26.
Results. We show that, during its outburst and also during its decay towards quiescence, Swift J1745−26 SED can be adjusted, from infrared up to X-rays, by the sum of both a viscous irradiated multi-colour black body emitted by an accretion disc, and a synchrotron power law at high energy. In the radio domain, the SED arises from synchrotron emission from the jet. While our SED fitting confirms that the source remained in the low/hard state during its outburst, we determine an X-ray spectral break at frequency 3.1 ≤ νbreak ≤ 3.4 × 1014 Hz, and a radio spectral break at 1012 Hz ≤ νbreak ≤ 1013 Hz. We also show that the system is compatible with an absorption AV of ∼7.69 mag, lies within a distance interval of D ∼ [2.6 − 4.8] kpc with an upper limit of orbital period Porb = 11.3 h, and that the companion star is a late spectral type in the range K0–M0 V, confirming that the system is a low-mass X-ray binary. We finally plot the position of Swift J1745−26 on an optical-infrared – X-ray luminosity diagram: its localisation on this diagram is consistent with the source staying in the low-hard state during outburst and decay phases.
Conclusions. By using new observations obtained at ESO/VLT with FORS2 and ISAAC, and adding archival optical (VLT/VIRCAM), radio and X-ray (Swift) observations, we built the light curve and the broad-band SED of Swift J1745−26, and we plotted its position on an optical-infrared – X-ray luminosity diagram. By fitting the SED, we characterized the emission of the source from infrared, via optical, up to X-ray domain, we determined the position of both the radio and X-ray spectral breaks, we confirmed that it remained in the low-hard state during outburst and decay phases, and we derived its absorption, distance interval, orbital period upper limit, and the late-type nature of companion star, confirming Swift J1745−26 is a low-mass X-ray binary.