bluuebunny/crossref_metadata_2025_split huggingface dataset:
Self-reported insomnia as a marker for anxiety and depression among migraineurs: a population-based cross-sectional study
Authors: Kim Kyung Min , Lee Dong Hyun , Lee Eun Ju , Roh Yun Ho , Kim Won-Joo , Cho Soo-Jin , Yang Kwang Ik , Yun Chang-Ho , Chu Min Kyung 
Published: December 2019

AbstractAnxiety, depression, and insomnia are highly prevalent among migraineurs and are associated with negative health consequences. Anxiety and depression, however, unlike insomnia, are usually underdiagnosed, due to less self-reporting of these two conditions. The aim of the present study was to evaluate the risk of anxiety and depression in migraineurs with self-reported insomnia, using a general population-based sample. We used data from a nationwide population-based survey on headache and sleep, the Korean Headache-Sleep Study. Of all 2,695 participants, 143 (5.3%), 268 (10.0%), 116 (4.3%), and 290 (10.8%) were classified as having migraine, anxiety, depression, and self-reported insomnia, respectively. The risk of anxiety (odds ratio [OR] = 7.0, 95% confidence interval [CI] = 3.0–16.7) and depression (OR = 3.3, 95% CI = 1.3–8.5) was significantly increased in migraineurs with self-reported insomnia. The sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) for anxiety in migraineurs with self-reported insomnia were 46.5%, 89.0%, 64.5%, and 79.5%, respectively. For depression, the sensitivity, specificity, PPV, and NPV were 41.7%, 82.4%, 32.3%, and 87.5%, respectively. Self-reported insomnia is likely to be comorbid with anxiety and depression in migraineurs and could thus be a useful predictor of anxiety and depression in migraine.
Shoulder tenderness was associated with the inflammatory changes on magnetic resonance imaging in patients with rheumatoid arthritis
Authors: Shinagawa Satoshi , Okamura Koichi , Yonemoto Yukio , Shitara Hitoshi , Suto Takahito , Sakane Hideo , Dam Trang Thuy , Tajika Tsuyoshi , Tsushima Yoshito , Takagishi Kenji , Chikuda Hirotaka 
Published: December 2019

AbstractThe aim of this study was to assess the association between the shoulder tenderness and the inflammatory changes on magnetic resonance imaging (MRI) in the rheumatoid shoulder. Forty-one shoulders of 41 patients with rheumatoid arthritis (RA) were examined. We evaluated synovitis, erosion and bone marrow edema, by counting the numbers of each positive site, and rotator cuff tears on shoulder MRI. The association between the shoulder tenderness and the MRI findings were statistically analyzed. Twenty-three of 41 patients had tenderness in the shoulder joints. There were 20 shoulders (48.8%) with rotator cuff tear, and no significant difference was observed in the prevalence of rotator cuff tear between the tenderness group and non-tenderness group (p = 0.080). There were no significant differences in the demographic data between these two groups. In MRI findings, we found significant difference for the synovitis (p = 0.001) and bone marrow edema (p = 0.021). Synovitis was strongly associated with the shoulder tenderness (OR: 3.996, 95% CI: 1.651–9.671). Synovitis was the factor most associated with shoulder tenderness.
Subducting serpentinites release reduced, not oxidized, aqueous fluids
Authors: Piccoli F. , Hermann J. , Pettke T. , Connolly J. A. D. , Kempf E. D. , Vieira Duarte J. F. 
Published: December 2019

AbstractThe observation that primitive arc magmas are more oxidized than mid-ocean-ridge basalts has led to the paradigm that slab-derived fluids carry SO2 and CO2 that metasomatize and oxidize the sub-arc mantle wedge. We combine petrography and thermodynamic modelling to quantify the oxygen fugacity (fO2) and speciation of the fluids generated by serpentinite dehydration during subduction. Silicate-magnetite assemblages maintain fO2 conditions similar to the quartz-fayalite-magnetite (QFM) buffer at fore-arc conditions. Sulphides are stable under such conditions and aqueous fluids contain minor S. At sub-arc depth, dehydration occurs under more reducing conditions producing aqueous fluids carrying H2S. This finding brings into question current models in which serpentinite-derived fluids are the cause of oxidized arc magmatism and has major implications for the global volatile cycle, as well as for redox processes controlling subduction zone geodynamics.
The Otranto Channel (South Adriatic Sea), a hot-spot area of plankton biodiversity: pelagic polychaetes
Authors: Guglielmo Rosanna , Bergamasco Alessandro , Minutoli Roberta , Patti Francesco P. , Belmonte Genuario , Spanò Nunziacarla , Zagami Giacomo , Bonanzinga Vincenzo , Guglielmo Letterio , Granata Antonia 
Published: December 2019

AbstractComposition, density and specimen sizes of pelagic polychaete assemblages were analyzed in the Southern Adriatic Sea. The study was based on finely stratified vertical (0–1100 m) and spatial sampling (17 stations) representing spring conditions. Holoplanktonic polychaetes were distributed in both neritic and pelagic waters, although the highest densities were observed along the Otranto Channel. Analysis of the size frequency distribution revealed a trend with depth only for some species. Spatial distribution of holoplanktonic polychaete density was not related to bottom depth, being the organisms mainly concentrated in the epipelagic layer (0–100 m). The most abundant species showed maximum values below or within the thermocline and within the Deep Chlorophyll Maximum or just above it. Relations between polychaete presence and the underlying oceanographic mechanisms regulating the circulation in the Otranto Channel were discussed. The presence of several non-determined polychaete larvae (e.g. Syllidae) in the pelagic waters at 800–1100 m depths suggests the importance of the role of Levantine waters as main actual and potential carrier of species in the area, though a relevant contribution comes also from North Adriatic dense waters through deep spilling and cascading in the Southern Adriatic pit. These findings increase the knowledge on holoplanktonic polychaetes ecology within the South Adriatic Sea, and represent significant data in the monitoring of changes in biodiversity.
Determining the duration of an ultra-intense laser pulse directly in its focus
Authors: Mackenroth Felix , Holkundkar Amol R. 
Published: December 2019

AbstractUltra-intense lasers facilitate studies of matter and particle dynamics at unprecedented electromagnetic field strengths. In order to quantify these studies, precise knowledge of the laser’s spatiotemporal shape is required. Due to material damage, however, conventional metrology devices are inapplicable at highest intensities, limiting laser metrology there to indirect schemes at attenuated intensities. Direct metrology, capable of benchmarking these methods, thus far only provides static properties of short-pulsed lasers with no scheme suggested to extract dynamical laser properties. Most notably, this leaves an ultra-intense laser pulse’s duration in its focus unknown at full intensity. Here we demonstrate how the electromagnetic radiation pattern emitted by an electron bunch with a temporal energy chirp colliding with the laser pulse depends on the laser’s pulse duration. This could eventually facilitate to determine the pulse’s temporal duration directly in its focus at full intensity, in an example case to an accuracy of order 10% for fs-pulses, indicating the possibility of an order-of magnitude estimation of this previously inaccessible parameter.
Lipid accumulation and oxidation in glioblastoma multiforme
Authors: Taïb Bouchra , Aboussalah Amine M. , Moniruzzaman Mohammed , Chen Suming , Haughey Norman J. , Kim Sangwon F. , Ahima Rexford S. 
Published: December 2019

AbstractGlioblastoma multiforme (GBM) is the most common and lethal primary malignant brain tumor in adults. Despite the multimodal standard treatments for GBM, the median survival is still about one year. Analysis of brain tissues from GBM patients shows that lipid droplets are highly enriched in tumor tissues while undetectable in normal brain tissues, yet the identity and functions of lipid species in GBM are not well understood. The aims of the present work are to determine how GBM utilizes fatty acids, and assess their roles in GBM proliferation. Treatment of U138 GBM cells with a monounsaturated fatty acid, oleic acid, induces accumulation of perilipin 2-coated lipid droplets containing triglycerides enriched in C18:1 fatty acid, and increases fatty acid oxidation. Interestingly, oleic acid also increases glucose utilization and proliferation of GBM cells. In contrast, pharmacologic inhibition of monoacylglycerol lipase attenuates GBM proliferation. Our findings demonstrate that monounsaturated fatty acids promote GBM proliferation via triglyceride metabolism, suggesting a novel lipid droplet-mediated pathway which may be targeted for GBM treatment.
Effects of different diets used in diet-induced obesity models on insulin resistance and vascular dysfunction in C57BL/6 mice
Authors: Lang Philipp , Hasselwander Solveig , Li Huige , Xia Ning 
Published: December 2019

AbstractThe aim of the present study was to compare different diets used to induce obesity in a head-to-head manner with a focus on insulin resistance and vascular dysfunction. Male C57BL/6J mice were put on standard chow diet (SCD), normal-fat diet (NFD), cafeteria diet (CAF) or high-fat diet (HFD) for 12 weeks starting at the age of 6 weeks. Both CAF and HFD led to obesity (weight gain of 179% and 194%, respectively), glucose intolerance and insulin resistance to a comparable extent. In aortas containing perivascular adipose tissue (PVAT), acetylcholine-induced vasodilation was best in the NFD group and worst in the CAF group. Reduced phosphorylation of endothelial nitric oxide synthase at serine 1177 was observed in both CAF and HFD groups. Plasma coagulation activity was highest in the HFD group and lowest in the SCD group. Even the NFD group had significantly higher coagulation activity than the SCD group. In conclusions, CAF and HFD are both reliable mouse diets in inducing visceral obesity, glucose intolerance and insulin resistance. CAF is more effective than HFD in causing PVAT dysfunction and vascular dysfunction, whereas hypercoagulability was mostly evident in the HFD group. Coagulation activity was higher in NFD than NCD group.
Effects of Chloride Concentration on the Water Disinfection Performance of Silver Containing Nanocellulose-based Composites
Authors: Lehtonen Janika , Hassinen Jukka , Honkanen Riina , Kumar Avula Anil , Viskari Heli , Kettunen Anu , Pahimanolis Nikolaos , Pradeep Thalappil , Rojas Orlando J. , Ikkala Olli 
Published: December 2019

AbstractThe availability of microbially-safe drinking water is a challenge in many developing regions. Due to the well-known antibacterial effect of silver ions, materials used for their controlled release have been widely studied for point-of-use water disinfection. However, even if it is in principle known that chloride anions can suppress the antibacterial efficiency of silver, the majority of previous studies, surprisingly, have not focused on chloride concentrations relevant for freshwaters and thus for practical applications. Here, we prepared low-cost nanocellulose-aluminium oxyhydroxide nanocomposites functionalized with silver nanoparticles. Field samples obtained from Chennai, India were used as a guideline for choosing relevant chloride concentrations for the antibacterial studies, i.e., 10, 90, and 290 ppm. The antibacterial performance of the material against Escherichia coli and Bacillus subtilis was demonstrated and the influence of chloride concentration on the antibacterial effect was studied with E. coli. A 1 h contact time led to bacterial reductions of 5.6 log10, 2.9 log10, and 2.2 log10, respectively. This indicates that an increase of chloride concentration leads to a substantial reduction of antibacterial efficiency, even within chloride concentrations found in freshwaters. This work enables further insights for designing freshwater purification systems that utilize silver-releasing materials.
An Automatic Assessment System for Alzheimer’s Disease Based on Speech Using Feature Sequence Generator and Recurrent Neural Network
Authors: Chien Yi-Wei , Hong Sheng-Yi , Cheah Wen-Ting , Yao Li-Hung , Chang Yu-Ling , Fu Li-Chen 
Published: December 2019

AbstractAlzheimer disease and other dementias have become the 7th cause of death worldwide. Still lacking a cure, an early detection of the disease in order to provide the best intervention is crucial. To develop an assessment system for the general public, speech analysis is the optimal solution since it reflects the speaker’s cognitive skills abundantly and data collection is relatively inexpensive compared with brain imaging, blood testing, etc. While most of the existing literature extracted statistics-based features and relied on a feature selection process, we have proposed a novel Feature Sequence representation and utilized a data-driven approach, namely, the recurrent neural network to perform classification in this study. The system is also shown to be fully-automated, which implies the system can be deployed widely to all places easily. To validate our study, a series of experiments have been conducted with 120 speech samples, and the score in terms of the area under the receiver operating characteristic curve is as high as 0.838.
Characterization of the pathogenesis and immune response to Listeria monocytogenes strains isolated from a sustained national outbreak
Authors: Ghosh Pallab , Zhou Yan , Richardson Quentin , Higgins Darren E. 
Published: December 2019

AbstractListeria monocytogenesis an intracellular pathogen responsible for listeriosis, a foodborne disease that can lead to life-threatening meningitis. The 2011L. monocytogenescantaloupe outbreak was among the deadliest foodborne outbreaks in the United States. We conductedin vitroandin vivoinfection analyses to determine whether strains LS741 and LS743, two clinical isolates from the cantaloupe outbreak, differ significantly from the common laboratory strain 10403S. We showed that LS741 and LS743 exhibited increased virulence, characterized by higher colonization of the brain and other organs in mice. Assessment of cellular immune responses to known CD8+T cell antigens was comparable between all strains. However, pre-existing immunity to 10403S did not confer protection in the brain against challenge with LS741. These studies provide insights into the pathogenesis of clinical isolates linked to the 2011 cantaloupe outbreak and also indicate that currently utilized laboratory strains are imperfect models for studyingL. monocytogenespathogenesis.
LXR-inverse agonism stimulates immune-mediated tumor destruction by enhancing CD8 T-cell activity in triple negative breast cancer
Authors: Carpenter Katherine J. , Valfort Aurore-Cecile , Steinauer Nick , Chatterjee Arindam , Abuirqeba Suomia , Majidi Shabnam , Sengupta Monideepa , Di Paolo Richard J. , Shornick Laurie P. , Zhang Jinsong , Flaveny Colin A. 
Published: December 2019

AbstractTriple-negative breast cancer (TNBC) is a highly aggressive subtype that is untreatable with hormonal or HER2-targeted therapies and is also typically unresponsive to checkpoint-blockade immunotherapy. Within the tumor microenvironment dysregulated immune cell metabolism has emerged as a key mechanism of tumor immune-evasion. We have discovered that the Liver-X-Receptors (LXRα and LXRβ), nuclear receptors known to regulate lipid metabolism and tumor-immune interaction, are highly activated in TNBC tumor associated myeloid cells. We therefore theorized that inhibiting LXR would induce immune-mediated TNBC-tumor clearance. Here we show that pharmacological inhibition of LXR activity induces tumor destruction primarily through stimulation of CD8+ T-cell cytotoxic activity and mitochondrial metabolism. Our results imply that LXR inverse agonists may be a promising new class of TNBC immunotherapies.
Application of a multiplex salivary immunoassay to detect sporadic incident norovirus infections
Authors: Wade Timothy J. , Griffin Shannon M. , Egorov Andrey I. , Sams Elizabeth , Hudgens Edward , Augustine Swinburne , DeFlorio-Barker Stephanie , Plunkett Trevor , Dufour Alfred P. , Styles Jennifer N. , Oshima Kevin 
Published: December 2019

AbstractNorovirus is one of the most common causes of gastroenteritis. Following infection, anti-norovirus salivary immunoglobulin G (IgG) rises steeply within 2 weeks and remains elevated for several months; this immunoconversion can serve as an indicator of infection. We used a multiplex salivary immunoassay to study norovirus infections among 483 visitors to a Lake Michigan beach in 2015. Saliva was collected on the day of the beach visit (S1); after 10–14 days (S2); and after 30–40 days (S3). Luminex microspheres were coupled to recombinant antigens of genogroup I (GI) and II (GII) noroviruses and incubated with saliva. Immunoconversion was defined as at least 4-fold increase in anti-norovirus IgG antibody response from S1 to S2 and a 3-fold increase from S1 to S3. Ten (2.1%) immunoconverted to either GI (2) or GII (8) norovirus. Among those who immunoconverted, 40% reported at least one gastrointestinal symptom and 33% reported diarrhea, compared to 15% (p = 0.06) and 8% (p = 0.04) among those who did not immunoconvert, respectively. The two participants who immunoconverted to GI norovirus both swallowed water during swimming (p = 0.08). This study demonstrated the utility of a non-invasive salivary immunoassay to detect norovirus infections and an efficient approach to study infectious agents in large cohorts.
Picoplankton accumulate and recycle polyphosphate to support high primary productivity in coastal Lake Ontario
Authors: Li Jiying , Plouchart Diane , Zastepa Arthur , Dittrich Maria 
Published: December 2019

AbstractPhytoplankton can accumulate polyphosphate (polyP) to alleviate limitation of essential nutrient phosphorus (P). Yet polyP metabolisms in aquatic systems and their roles in P biogeochemical cycle remain elusive. Previously reported polyP enrichment in low-phosphorus oligotrophic marine waters contradicts the common view of polyP as a luxury P-storage molecule. Here, we show that in a P-rich eutrophic bay of Lake Ontario, planktonic polyP is controlled by multiple mechanisms and responds strongly to seasonal variations. Plankton accumulate polyP as P storage under high-P conditions via luxury uptake and use it under acute P stress. Low phosphorus also triggers enrichment of polyP that can be preferentially recycled to attenuate P lost. We discover that picoplankton, despite their low production rates, are responsible for the dynamic polyP metabolisms. Picoplankton store and liberate polyP to support the high primary productivity of blooming algae. PolyP mechanisms enable efficient P recycling on ecosystem and even larger scales.
Availability of orchid mycorrhizal fungi on roadside trees in a tropical urban landscape
Authors: Izuddin Muhammad , Srivathsan Amrita , Lee Ai Lan , Yam Tim Wing , Webb Edward L. 
Published: December 2019

AbstractUrban expansion threatens biodiversity worldwide, therefore urban spaces need to be amenable to biodiversity conservation. On trees in urban environments, natural colonisation and successful translocation of epiphytic orchids are necessary to enhance urban biodiversity, and depend on the availability of compatible orchid mycorrhizal fungi (OMF). However, the extent of OMF presence and distribution, as well as niche requirements for the OMF, remain poorly studied. To identify and quantify OMF on urban trees as well as assess their suitability for native epiphytic orchids, we conducted high-throughput sequencing on tree bark and orchid root samples. OMF were detected at 60% of the study sites on 16% of 270 bark samples (from stem, fork, and branch microsites within each tree). OMF presence and richness on bark samples were related to multiple biophysical factors; in general, humus presence and precipitation levels were positively predictive of OMF presence and richness. We found Ceratobasidiaceae- and Serendipitaceae-associated OMF both on bark and within roots. Orchid species also showed differing mycorrhizal specificity. Sites associated with fungal generaCeratobasidium,Rhizoctonia, andSerendipitawere considered suitable habitats for seven orchid species. The results suggest that urban trees support OMF and are therefore suitable for native orchid species; however, OMF availability are largely constrained by biophysical factors. To maximise the likelihood of translocation success and consequent natural establishment, we propose that (micro)sites are screened for compatible OMF prior to any intervention.
Microwave-Assisted Tunneling in Hard-Wall InAs/InP Nanowire Quantum Dots
Authors: Cornia Samuele , Rossella Francesco , Demontis Valeria , Zannier Valentina , Beltram Fabio , Sorba Lucia , Affronte Marco , Ghirri Alberto 
Published: December 2019

AbstractWith downscaling of electronic circuits, components based on semiconductor quantum dots are assuming increasing relevance for future technologies. Their response under external stimuli intrinsically depend on their quantum properties. Here we investigate single-electron tunneling in hard-wall InAs/InP nanowires in the presence of an off-resonant microwave drive. Our heterostructured nanowires include InAs quantum dots (QDs) and exhibit different tunnel-current regimes. In particular, for source-drain bias up to few mV Coulomb diamonds spread with increasing contrast as a function of microwave power and present multiple current polarity reversals. This behavior can be modelled in terms of voltage fluctuations induced by the microwave field and presents features that depend on the interplay of the discrete energy levels that contribute to the tunneling process.
Distinct metabolic profiles in Drosophila sperm and somatic tissues revealed by two-photon NAD(P)H and FAD autofluorescence lifetime imaging
Authors: Wetzker Cornelia , Reinhardt Klaus 
Published: December 2019

AbstractMetabolic profiles vary across all levels of biological diversity, from cells to taxa. Two-photon fluorescence lifetime imaging microscopy (FLIM) facilitates metabolic characterisation of biological specimens by assaying the intrinsic autofluorescence of the ubiquitous coenzymes NAD(P)H and FAD. The potential of this method for characterising the diversity of organismal metabolism remains largely untapped. Using FLIM in Drosophila melanogaster, we show tissue-specificity in fluorescence lifetime that reflects variation in redox patterns. In particular, sperm cells exhibited elevated glycolysis relative to other tissues. We also show that sperm metabolism is phenotypically plastic: compared to male-stored sperm, sperm stored in the female’s storage organ showed a substantial reduction in the protein-bound FAD lifetime fraction but no change in the NAD(P)H profile. This study represents the first ex vivo investigation of sperm metabolism using FLIM.