bluuebunny/crossref_metadata_2025_split huggingface dataset:
The association of chronic anxiousness with cardiovascular disease and mortality in the community: results from the Gutenberg Health Study
Authors: Reiner Iris C. , Tibubos Ana N. , Werner Antonia M. , Ernst Mareike , Brähler Elmar , Wiltink Jörg , Michal Matthias , Schulz Andreas , Wild Philipp S. , Münzel Thomas , Arnold Natalie , Mahmoudpour Seyed Hamidreza , Lackner Karl J. , Pfeiffer Norbert , Beutel Manfred E. 
Published: July 2020

AbstractIn a large German community sample of adults, we investigated the association of chronic anxiousness with cardiovascular disease and mortality. Self-reported anxiousness from 11,643 German adults between 40 and 80 years of age from the Gutenberg Health Study (GHS) was analyzed over 5 years. Multivariable regression modeling assessed the relation between the variables, cardiovascular disease and mortality. Twelve percent of the participants reported consistently raised (chronic) anxiousness over at least 2.5 years. Anxiousness was more often reported by female, younger participants with a lower socioeconomic status, smokers and those with a family history of stroke and myocardial infarction. New onset of cardiovascular disease was linked to chronic anxiousness in men and new onset of anxiousness in women. However, chronic anxiousness did not predict all-cause mortality. Our results revealed that anxiousness is highly prevalent in German adults from middle to old age, affecting women in particular. In our study, we found sex-specific associations between new onset of cardiovascular disease and different forms of anxiousness in men and women. We suggest that even subclinical levels of anxiety need to be considered as cardiovascular risk factors. To elucidate potential harm of anxiousness for mental and physical health, we propose sex-specific analyses in further research studies, taking age and the course of anxiousness into account.
Role of hyperhomocysteine, thyroid dysfunction and their interaction in ischemic stroke patients with non-valvular atrial fibrillation
Authors: Wang Lili , Zhang Yun 
Published: July 2020

AbstractThe role of hyperhomocysteinemia (HHcy) and thyroid dysfunction in ischemic stroke with non-valvular atrial fibrillation (AF) remains controversial. Till now, the relationship between HHcy and thyroid dysfunction in ischemic stroke with non-valvular AF has not been reported. The aim of this study was to investigate the role and relationship of HHcy and thyroid dysfunction in ischemic stroke patients with non-valvular AF. Seven hundred and seventeen patients with acute ischemic stroke within the first 7 days of symptom onset were consecutively included in this study. Eligible patients were divided into AF group and without AF group. Variables including age, sex, smoke, drink, history of stroke were collected. Levels of homocysteine (Hcy), thyroid-stimulating hormone were evaluated at presentation. Multivariable logistic regression and spearman bivariate correlate analysis were used to evaluate the relationship of HHcy and thyroid dysfunction in ischemic stroke patients with AF. There were 122 patients with AF and 595 patients without AF. Two hundred and eighty-eight (40.2%) patients had HHcy and Three hundred and sixteen (44.1%) patients had thyroid dysfunction. There was significant difference of serum Hcy (P = 0.014) and thyroxine levels (P = 0.002) between patients with and without AF. Furthermore, the difference of serum Hcy (P = 0.007) and thyroxine levels (P = 0.004) between patients with and without AF was also significant in female subgroups. We did not find association between HHcy and thyroid dysfunction in AF group (P = 0.463). In conclusion, both HHcy and thyroid dysfunction were associated with AF in patients with acute ischemic stroke and in female subgroup patients. However, thyroid dysfunction had no relationship with HHcy in ischemic stroke patients with AF.
HN1 as a diagnostic and prognostic biomarker for liver cancer
Authors: Liu Zhicheng , Yang Dingquan , Li Yanqing , Jiao Yan , Lv Guangchao 
Published: July 2020

Abstract
Background: The present study aimed to examine the diagnostic and prognostic value of HN1 in terms of overall survival (OS) and recurrence-free survival (RFS) in liver cancer and its potential regulatory signaling pathway.
Methods: We obtained clinical data and HN1 RNA-seq expression data of liver cancer patients from The Cancer Genome Atlas database, and analyzed the differences and clinical association of HN1 expression in different clinical features. We uesd receiver-operating characteristic curve to evaluate the diagnosis capability of HN1. We analyzed and evaluated the prognostic significance of HN1 by Kaplan–Meier curves and Cox analysis. Gene Set Enrichment Analysis (GSEA) was used to identify signaling pathways related to HN1 expression.
Results: HN1 mRNA was up-regulated in liver cancer, and was associated with age, histologic grade, stage, T classification, M classification, and vital status. HN1 mRNA had ideal specificity and sensitivity for the diagnosis (AUC = 0.855). Besides, the analysis of Kaplan–Meier curves and Cox model showed that HN1 mRNA was strongly associated with the overall survival and could be well-predicted liver cancer prognosis, as an independent prognostic variable. GSEA analysis identified three signaling pathways that were enriched in the presence of high HN1 expression.
Conclusion: HN1 serves as a biomarker of diagnosis and prognosis in liver cancer.
Exploration of the effects of goose TCs on GCs at different follicular stages using a co-culture model
Authors: Gan Xiang , Wang Yushi , Gao Shanyan , Chen Xi , Hu Shenqiang , Wang Jiwen , Hu Jiwei , Li Liang , Han Chunchun 
Published: July 2020

Abstract
Granulosa cells (GCs) play a critical role in follicular development, which cannot be separated from the assistance of theca cells (TCs). In the present study, we used a transwell system to develop three stages of goose GCs in vitro mono-culture and co-culture models, and we analyzed the morphology, activity, intracellular lipid content and the expression of core genes involved in de novo lipogenesis (DNL), steroidogenesis, proliferation and apoptosis of the GCs. In the co-culture group, the activity of all three stages of GCs showed significant (P&lt;0.01) changes, and they had a strong (P&lt;0.01) correlation with culture time; further, the intracellular lipid deposition of hierarchical GCs was significantly different (P&lt;0.01) between the two methods. Moreover, after co-culture, in pre-hierarchical GCs, the expression of SREBP, CYP11 and 3βHSD was promoted (P&lt;0.01). In hierarchical GCs, the expression of ACC, SREBP, STAR, CYP11, 3βHSD and CCND1 was promoted at 48 h, but they were inhibited (P&lt;0.05) at 96 h. In F1 GCs, the expression of ACC, FAS, SREBP, CYP11, BCL2 and CAS3 was inhibited (P&lt;0.01). The results indicate that goose TCs had complex and time-dependent effects on the biological function of GCs at each corresponding stage, and the effects were distinct in the different stages. In addition, DNL, steroidogenesis, proliferation and apoptosis in hierarchical and F1 GCs might have some synergistic relationships in the effects of TCs on GCs. Furthermore, we speculated that TCs might play an important role in the differentiation and maturation of GCs during follicular development.
Comparison of a novel cholesterol efflux assay using immobilized liposome-bound gel beads with the conventional method
Authors: Horiuchi Yuna , Lai Shao-Jui , Kameda Takahiro , Tozuka Minoru , Ohkawa Ryunosuke 
Published: July 2020

Abstract
Cholesterol efflux capacity (CEC) is an atheroprotective function of high-density lipoprotein (HDL). CEC is currently measured using artificially prepared foam cells composed of cultured macrophage and 3H-cholesterol. However, this conventional method is not suitable for clinical laboratory use due to poor repeatability, complexity, and low safety. Recently, we reported a novel CEC assay, called the immobilized liposome-bound gel beads (ILG) method. The ILG method is an alternative to foam cells, comprising gel beads and 4,4-diflioro-4-bora-3a,4a-s-indacene labeled cholesterol (BODIPY-cholesterol) instead of macrophage and 3H-cholesterol, respectively. The ILG method has shown adequate basic properties and strong correlation with the conventional method. Here, we aimed to compare this new ILG method with the conventional method in-depth. When apoB-depleted serum was used as the cholesterol acceptor (CA), the ILG method had far better reproducibility than the conventional method. The CEC of major HDL subclasses HDL2 and HDL3 had similar results in both the ILG and conventional method. However, the ILG method did not reflect the CEC of apolipoprotein (apo) A–I and a minor HDL subclass which uses ATP-binding cassette transporter A1 on foam cells. Superior reproducibility of the ILG method, which is a limitation of the conventional method, and similar CEC results for major HDL subclasses in the ILG and conventional methods, provide further evidence that the ILG method is promising for measuring CEC clinically. However, some HDL subclasses or apo might have poor CEC correlation between these methods. Further research is therefore needed to confirm the clinical significance of estimating CEC by the ILG method.
Seismic solar models from Ledoux discriminant inversions
Authors: Buldgen G. , Eggenberger P. , Baturin V. A. , Corbard T. , Christensen-Dalsgaard J. , Salmon S. J. A. J. , Noels A. , Oreshina A. V. , Scuflaire R. 
Published: July 2020

Context. The Sun constitutes an excellent laboratory of fundamental physics. With the advent of helioseismology, we were able to probe its internal layers with unprecendented precision and thoroughness. However, the current state of solar modelling is still stained by tedious issues. One of these central problems is related to the disagreement between models computed with recent photospheric abundances and helioseismic constraints. The observed discrepancies raise questions on some fundamental ingredients entering the computation of solar and stellar evolution models.
Aims. We used solar evolutionary models as initial conditions for reintegrating their structure using Ledoux discriminant inversions. The resulting models are defined as seismic solar models, satisfying the equations of hydrostatic equilibrium. These seismic models will allow us to better constrain the internal structure of the Sun and provide complementary information to that of calibrated standard and non-standard models.
Methods. We used inversions of the Ledoux discriminant to reintegrate seismic solar models satisfying the equations of hydrostatic equilibrium. These seismic models were computed using various reference models with different equations of state, abundances, and opacity tables. We checked the robustness of our approach by confirming the good agreement of our seismic models in terms of sound speed, density, and entropy proxy inversions, as well as frequency-separation ratios of low-degree pressure modes.
Results. Our method allows us to determine the Ledoux discriminant profile of the Sun with an excellent accuracy and compute full profiles of this quantity. Our seismic models show an agreement with seismic data of ≈0.1% in sound speed, density, and entropy proxy after seven iterations in addition to an excellent agreement with the observed frequency-separation ratios. They surpass all standard and non-standard evolutionary models including ad hoc modifications of their physical ingredients that aim to reproduce helioseismic constraints.
Conclusions. The obtained seismic Ledoux discriminant profile, as well as the full consistent structure obtained from our reconstruction procedure paves the way for renewed attempts at constraining the solar modelling problem and the missing physical processes acting in the solar interior by breaking free from the hypotheses of evolutionary models.
The influence of environmental factors and heavy metals in the soil on plants’ growth and development
Authors: Vanghele Nicoleta Alexandra , Pruteanu Mirabela Augustina , Petre Ancuţa Alexandra , Matache Andreea , Mihalache Dumitru Bogdan , Stanciu Mariana Mădălina 
Published: July 2020

Plants need certain conditions that represent their living environment. When the living environment provides the conditions required by the plant, it will grow and develop properly. The growth and development of plants involve environmental factors, which represent those constituent elements of the natural environment, which actively intervene in plants’ life.
The present work shows the characteristics of an agricultural soil, contaminated with heavy metals (Cu, Pb and Zn) in different concentrations, which has been divided into pots, in which were thereafter planted vegetable seedlings (tomatoes, cucumbers, parsley, spinach, carrots, radishes). During the plants’ growing time, the temperature and humidity of the air inside the greenhouse, as well as the humidity and pH of the soil, were monitored. The growth and the development of the plants under certain conditions were also tracked, until the end of the growing period. The results of monitoring the plants’ growth and development are important in assessing the impact of the contamination over the soil and the plants.
Study on the effects and changes of soil degradation under the influence of antibiotics
Authors: Petre Ancuta Alexandra , Nenciu Florin , Vanghele Nicoleta Alexandra , Stanciu Mariana Mădălina , Mihalache Dumitru Bogdan , Grigore Iulia Andreea , Vladuţoiu Laurenţiu 
Published: July 2020

The use of antibiotics in large quantities against the combat of pests in soils, indicates an increased remanence of them, which leads to major environmental risks. After entering in the soil, antibiotics are subjected to a succession of biogeochemical processes under the action of multiple environmental factors: absorption, migration, transformation, degradation or nutritional prosperity of plants.
In order to know the current state of the environment and the effects of antibiotics it is essential to discover procedures for improving the degradation and combating the dissemination of antibiotic resistance.
Research in recent years on the extraction of antibiotics from the soil is based on complex processes, such as: Soxhlet extraction, ultrasonic extraction or accelerated solvent extraction.
Soil residue was determined using liquid chromatography coupled with mass spectrometry, being the equipment that provides the highest accuracy in the analysis of polar compounds in very low concentrations.
The purpose of the paper is to find an efficient and ecological solution for the elimination of antibiotics from soils and to highlight the evaluation of the contaminated land regarding the antibiotic residues and the impact on the composition of the bacterial community. Information about the effects of antibiotics accumulated in soil, persistence, resistance and sensitivity to chemical and microbiological substances, degradation and results related to soil fertility and crop production are highlighted.
Prostate-Specific Antigen Density: A Measurement to Differentiate Benign Hypertrophy of Prostate from Prostate Carcinoma
Authors: Nath Chandan Kumar , Barman Bhupen , Phukan Pranjal , Sailo Stephen L. , Dey Biswajit , Nath Indrajit , Rajkhowa Purnima 
Published: July 2020

AbstractBackground Determination of isolated prostate-specific antigen (PSA) in asymptomatic individuals has not demonstrated sufficient sensitivity and specificity to be useful in the routine evaluation of prostate disease. To enhance the accuracy of serum PSA we have used a proportion of serum PSA and prostate volume, which we refer to as prostate-specific antigen density (PSAD). Prostate volume in this study was calculated using transrectal ultrasonography (TRUS).Materials and Methods A total of 106 patients with prostatic disease clinically confined to the prostate glands were evaluated.Results and Observation The mean PSAD for prostate cancer was 0.15 ± 0.01 while that for benign hypertrophy of the prostate (BPH) was 0.11 ± 0.02 (p < 0.05). Significant difference (p < 0.05) was noted in the prostate volume in these two groups with the mean prostate volume measured by TRUS in the BPH to be 53.85 ± 9.71 mL compared with 58.14 ± 7.48 mL in the carcinoma. PSA density of 0.13 ng/mL can be used as a cutoff for the individual in our set-up who should go for prostate biopsy with sensitivity and specificity of over 90%.Conclusion These results suggest that PSAD may be useful in distinguishing BPH and prostate cancer.
Nephrology and Coronavirus Disease 2019
Authors: Bharati Joyita , Ramachandran Raja 
Published: July 2020

AbstractNovel coronavirus disease 2019 (COVID-19) has emerged as a threat to the human population worldwide. Old age and presence of comorbidities are risk factors for severe complications of the disease, as many of these patients are not able to mount effective or have an aberrant antiviral response. Patients with chronic kidney disease are at high risk of COVID-19 and its complications, especially those with end-stage renal disease (ESRD). Patients have repeated exposure to crowding in dialysis units, and face the uncertainties of health care system sustainability during periods of increasing demand and scarce supply. Acute kidney injury (AKI) is a notable complication in patients with COVID-19 with an incidence of up to 25% in specific populations. Patients with AKI have a higher mortality rate. The etiology and pathogenesis of AKI in COVID-19 are multifactorial. Direct viral invasion and cytopathic effects on renal tubular cells and indirect factors like hypovolemia, rhabdomyolysis, renal vein congestion, and hypercoagulable state play a role in causing AKI in these patients. Renal replacement therapy in the form of continuous renal replacement therapy is most widely used worldwide mostly due to hemodynamic instability of critically sick patients with COVID-19. Anticoagulation therapy is critical as most COVID-19 patients have increased clotting tendency and extracorporeal circuit thrombosis is common. ESRD patients in China had mild-to-moderate symptoms commonly. However, the data from Spain and Italy report a higher mortality rate in ESRD patients than the general population. Strict screening and uniform adherence to infection control practices have led to a decreased risk of cross-infection among other patients and dialysis personnel in dialysis units. The association between nephrology and COVID-19 is unique and focus on further research on kidney pathology along with the strengthening of existing dialysis facilities are needed.
BaGa2GeS6 and BaGa2GeSe6 crystals for nonlinear optical frequency conversion
Authors: Grechin S.G. , Nikolaev P.P. , Ionin A.A. , Kinyaevskii I.O. , Andreev Yu.M. 
Published: July 2020

Abstract
We analyse the functional capabilities of new crystals, BaGa2GeS6 (BGGS) and BaGa2GeSe6 (BGGSe), which are used for nonlinear optical frequency conversion in their transparency range. The wavelengths at which maximum conversion efficiencies can be obtained and the tuning range for difference-frequency generation are found. It is shown that there are wavelength combinations at which the effective nonlinearity coefficient varies only slightly in a wide frequency band.
Scheme for stabilising the phase and arrival time of ultrashort laser pulses in a coherent beam combining fibre system
Authors: Andrianov A.V. , Korobeynikova A.P. 
Published: July 2020

Abstract
An algorithm has been demonstrated for simultaneously measuring the phase and arrival time of optical pulses relative to pulses in a reference channel using one detector array. Based on this algorithm, a simple optoelectronic pulse phase and arrival time stabilisation scheme has been designed for operation in a coherent beam combining fibre system for ultrashort pulses (including chirped ones). We have studied the performance of the stabilisation scheme. In particular, we have reached a phase measurement rate of 25 000 cps, demonstrated an ∼ 2 kHz limiting phase stabilisation frequency in a feedback loop, and obtained an arrival time measurement accuracy of ∼ 5 % of the transform-limited pulse duration at a measurement rate of 5 cps. In experiments concerned with coherent beam combining for two channels of a pulsed fibre system at a wavelength of 1030 nm, we have demonstrated phase stabilisation with an accuracy better than λ/100 and delay setting accuracy of 10 fs at a transform-limited pulse duration of ∼ 200 fs and obtained 94 % beam combining efficiency.
Single administration of resveratrol improves social behavior in adult mouse models of autism spectrum disorder
Authors: Hidema Shizu , Kikuchi Shohei , Takata Ryoji , Yanai Takaaki , Shimomura Kenju , Horie Kengo , Nishimori Katsuhiko 
Published: July 2020

Abstract
Resveratrol (RSV) is a natural polyphenol present in grapes, the skin of peanuts, and several other plants with many health benefits. Autism spectrum disorder (ASD) is a neurodevelopmental disorder that may be linked to neural and synaptic development impairments. The present study aimed to analyze the preventive effects of RSV on the development of ASD-like behavior, using oxytocin receptor gene knockout (Oxtr-KO) and valproic acid-induced ASD (VPA-ASD) model mice. Genetic deficiencies in Oxtr are suggested to be involved in ASD etiology. Twenty-four hours after a single RSV injection to the Oxtr-KO mice, the social impairments caused by OXTR deficiency were ameliorated. RSV also improved social impairments in the VPA-ASD mice. Administration of RSV up-regulated silent information regulator 1 (Sirt1) gene and early growth response factor 3 (Egr3) gene expressions in the amygdala of the Oxtr-KO mice. Our data suggest that RSV may have therapeutic effects on ASD with multiple targets.
Acute effects of lactic acid-fermented and enzyme-digested soybean on protein synthesis via mTOR signaling in the skeletal muscle
Authors: Ono Kohei , Tanaka Minoru , Ikeji Takuya , Nakanishi Ryosuke , Hirabayashi Takumi , Tategaki Airo , Kondo Hiroyo , Ishihara Akihiko , Fujino Hidemi 
Published: July 2020

Abstract
Protein-containing nutrients result in the efficient hypertrophy of muscles by increasing muscle protein synthesis. Soybean is often ingested by athletes or individuals who exercise; however, it takes very long to be absorbed. Lactic acid-fermented and enzyme-digested (LFED) soybean is absorbed faster than untreated soybean. This study aims at determining muscle protein synthesis after ingesting a single bolus of soybean or LFED soybean produced by lactic acid bacteria and protease digestion. Eight-week-old overnight-fasted ICR mice were administered powdered or LFED soybean. Mice were euthanized at 7, 15, 30, 60, 90, and 120 min after soybean intake. We have demonstrated that LFED soybean administration was quicker in stimulating muscle protein synthesis by activating mammalian target of rapamycin (mTOR) signaling than orally ingesting untreated soybean in the gastrocnemius muscle. These results suggested that LFED soybean is a more efficient source of nutrition for muscle hypertrophy than untreated soybean.