bluuebunny/crossref_metadata_2025_split huggingface dataset:
Liposomal clodronate combined with Cisplatin or Sorafenib inhibits hepatocellular carcinoma cell proliferation, migration and invasion by suppressing FOXQ1 expression
Authors: Zhou Yilong , Chen Yuan , Ma Chunyang , Shao Bingfeng , Zhang Feng 
Published: May 2020

The purpose of this study was to investigate the effect of liposomal clodronate combined with Cisplatin or Sorafenib on the FOXQ1 expression and biological function of hepatocellular carcinoma cell lines. The expression of FOXQ1 was determined in normal hepatic cell line and hepatocellular carcinoma cell lines using quantitative real-time polymerase chain reaction (qRT-PCR). HepG2 and MHCC97H cells were administered low, medium and high concentrations of cisplatin (3, 5 and 7 Î¼g/ml) or Sorafenib (2, 7 and 20 Î¼g/ml) in combination with liposomal clodronate (LC, 20Î¼g/ml), and the expression of FOXQ1 in each group was determined. Cell migration, MTT and transwell assays were used to determine the effects of the treatments on biological functions of HepG2 and MHCC97H cells. qRT-PCR showed that the expression of FOXQ1 mRNA was higher in the four hepatocellular carcinoma cell lines than in normal cells, and the expression of FOXQ1 mRNA in HepG2 and MHCC97H cells were more dominant. All the tested doses of Cisplatin, but only high dose Sorafenib down-regulated the expression of FOXQ1. However, Sorafenib at low and medium concentrations had no significant effect on the expression of FOXQ1. When Cisplatin or Sorafenib was administered in combination with LC, the expression level of FOXQ1 was significantly reduced. Cell migration, MTT and transwell assays showed that proliferation, migration and invasion were inhibited when each drug was administered alone, but was stronger when the drugs were combined with liposomal clodronate.Liposomal clodronate combined with Cisplatin or Sorafenib down-regulates the expression of FOXQ1 in HepG2 and MHCC97H hepatoma cells, and inhibits their proliferation, migration and invasion.
Molecular Barcoding Reveal the Existence of Mole Crabs Emerita emeritus in North Coast of Central Java
Authors: Bhagawati Dian , Winarni Elly Tuti , Nuryanto Agus 
Published: May 2020

The existence of mole crabs had been reported from the southern coast of Central Java. However, no mole crab data was available from the northern coast Central Java. This study aimed to figure out the existence of mole crabs in the northern coast of Central Java as revealed from the cytochrome c oxidase 1 (COI) barcoding. Mole crabs samples collected in Sendang Sikucing Beach Kendal. Taxonomic status of the samples was inferred from sequences similarity test using basic local alignment search tool to conspecific sequence deposited in GenBank. Pairwise genetic distances were calculated based on the Kimura 2-parameter model. A phylogenetic tree had reconstructed in molecular evolution genetic analysis (MEGA) software based on neighbor-joining algorithm. Branching reliability was obtained from 1000 bootstraps replication while branching polarity was obtained from the out-group comparison. The mole crab samples from the northern coast of Central Java had unambiguously identified as Emerita emeritus based on high sequences similarities (98.27%-99.70%), low genetic distances (0.002-0.005), and their monophyly with Emerita emeritus (KR047035) in GenBank. Pairwise analysis among each possibility pair of samples had genetic distances ranged between 0.000 and 0.005, indicated that all sample belong to single species. The data provide the first record of the existence of Emerita emeritus in the northern coast of Central Java. Our data on the existence of E. emeritus in the northern coast of Central Java is among essentials information as a scientific basis in creating policy for the management of sustainable use of the mole crabs in the areas.
Effect of Induced Polyploidy on Plant Growth, Chlorophyll and Flavonoid Content of Artemisia cina
Authors: Kasmiyati Sri , Kristiani Elizabeth Betty Elok , Herawati Maria Marina 
Published: May 2020

Artemisia cina is one of a member of genus Artemisia that has potential as a medicinal plant. However, the levels of Artemisia medicinal bioactive compounds are very low. Polyploidization is an alternative method that can enhance of growth and secondary metabolite productions of plants. The aims of this research were to determine the effect of polyploid induction using colchicine and plant growth regulator toward plant growth, the chlorophyll, kaemferol and quercetin contents of A. cina. Four different A. cina used in this research consisted of two diploid genotypes (TWN and KJT) and two polyploid genotypes (J and M). Induction of mutant polyploid was conducted using colchicine and combination of plant growth regulator benziladenyl (BA) and 2,4-dichlorophenoxyacetic acid (2,4-D). The measured plant growth parameters were dry weight, leaf area, and plant height. The chlorophyll content of leaves was determined spectrophotometrically, and flavonoid content determined using HPLC. The result showed that the polyploid genotype (M) significantly decreased leaf and root growth compared to the KJT and TWN. In A. cina plants, polyploidization using colchicine is more effective in increasing the biomass than using combination plant growth regulator BA and 2,4-D. The flavonoid content of KJT was the lowest, and significantly different compared to the other plants. This study provides new information about the effect of polyploid on growth and flavonoid content in A. cina. This can be useful information to develop A. cina to become a medicinal plant.
Tyrosinase Inhibition, Antiglycation, and Antioxidant Activity of Xylocarpus granatum
Authors: Batubara Irmanida , Mustofa Maily , Wahyuni Wulan Tri , Tilaar Kilala , Nurcholis Waras , Junardy Fransiska Devy , Priyadi Yogo Suro , Subroto Erna , Egra Saat , Zamany Nevianti 
Published: May 2020

Xylocarpus granatum is mangrove plant that traditionally used as face powder in Central Sulawesi, Indonesia which related to antioxidant, antiglycation and tyrosinase inhibition activities. This study aimed to evaluate the potency of X. granatum as a tyrosinase inhibitor, antiglycation, and antioxidant. The leaves, stem, stem bark, fruit flesh, fruit peel, and kernel of X. granatum were extracted using ethanol then their tyrosinase inhibition, antiglycation, and antioxidant were evaluated. Tyrosinase inhibition activity was evaluated using in vitro assay with L-tyrosine and L-DOPA as the substrate of monophenolase and diphenolase. Antiglycation activity was studied by measuring the excitation and emission fluorescence from glucose and fructose reaction with Bovine Serum Albumin. Antioxidant activity was measured using the DPPH radical scavenging assay. The result showed that the ethanolic extract of fruit flesh has higher potency as tyrosinase inhibitor (IC50 of 393.8 mg/L and IC50 of 448 mg/L, respectively for monophenolase and diphenolase). Antiglycation assay showed that the ethanolic extract of stem bark provides the strongest antiglycation activity with an IC50 of 118.1 mg/L. Meanwhile, fruit peel provides the strongest antioxidant activity with an IC50 of 5.5 mg/L. Fractionation of ethanolic extracts of each part of X. granatum tree yield fractions with lower bioactivity compared to the crude extract. Moreover, stem extract and fractions from two different locations (Tarakan and Kendari) tend to have different bioactivities strengths. The stem part of X granatum could be developed as new raw material of cosmetic product in Indonesia, while ethanol as the solvent for extraction, and the different bioactivity of stem extract from different location can be the consideration for the industry to standardize the extract prior to production of final product.
Microbiological Quality and Proximate Composition of Bakasang Laor, a Traditional Fermented Fishery Product in Maluku
Authors: Mahulette Ferymon , Kurnia Tri Santi 
Published: May 2020

Bakasang laor is one of the traditional fermented fishery products in Maluku, Indonesia. There are two types of bakasang laor, i.e. with and without vinegar. The microbiological research of bakasang processed use laor as raw material has never been done before. The research aimed to analyze the microbiological quality and proximate composition of two types of bakasang laor. The proximate composition can determine the nutritional value of this product for consumption by the community. The sample of bakasang laor was taken from traditional producer in Latuhalat village, Ambon. The total number of halotolerant and coliform bacteria in bakasang laor without vinegar were 1.7x106 CFU/g and 4.1x106 CFU/g, respectively, while the total of lactic acid bacteria in bakasang laor with vinegar was 6.0x106 CFU/g. The carbohydrate, protein, fat and ash contents of bakasang laor with vinegar was higher than bakasang laor without vinegar. The carbohydrate, protein and fat contents of bakasang laor with vinegar were 19.64 %, 16.23 %, and 2.42 %, respectively. Generally, microbiological quality and proximate composition of bakasang laor with vinegar was better than bakasang laor without vinegar. The bacteria that play a role in fermentation can be used as a starter in the fermentation of various food products. The results of this research to improve the quality of this fermentation product in the future.
Emancipating lenes
Authors: Szigetvári Péter 
Published: May 2020

AbstractI argue that English has no voicing assimilation, in fact, it does not have phonologically voiced segments at all. Voicing in English is spontaneous in sonorants, while obstruents may be phonetically voiced only if lenis and surrounded by spontaneously or passively voiced sounds. The paper claims that most obstruent clusters of English are traditionally misanalysed as fortis+fortis clusters. These clusters are all either fortis+lenis or lenis+fortis; in fact, fortis+fortis clusters are completely ruled out in English.
Boyun, Bel ve Diz Ağrısı Olan Yaşlılarda Fizik Tedavi ve Balneoterapi, Retrospektif Çalışma
Authors: DİLEKÇİ Erdal , ÖZKUK Kağan 
Published: May 2020

Amaç:Çalışmanın amacı,
boyun bel ve diz ağrısı olan yaşlı bireylerde balneoterapinin fizik tedaviye
olan katkısını incelemektir.Gereç
ve yöntem: Bu retrospektif çalışmada, 2013 ve 2018 yılları arasında
fizik tedavi veya fizik tedavi+balneoterapi alan hastaların kayıtları
incelendi. Tespit edilen 704 yaşlı hasta arasından değerlendirme parametreleri
tam olan 482 hastanın verileri analiz edildi. Bulgular: Hastaların
196sına sadece fizik tedavi uygulanmıştı (grup 1). 286 hasta hem fizik hemde
balneoterapi uygulanmıştı(grup2). 173 hasta boyundan, 338 hasta belden, 287
hasta belden tedavi almıştı. Genel değerlendirmede 482 hastada Ağrı Görsel
Analog Ölçeği (VAS) ve 276 hastada Sağlık Değerlendirme Anketi (HAQ), boyundan
tedavi alan 173 hastada VAS, 120 hastada HAQ,
belden tedavi alan 338 hastada VAS, 238 hastada HAQ ve dizden tedavi
alan 287 hastada VAS, 177 hastada HAQ, sonuçları incelendiğinde tedaviden sonra
değerlendirilen tüm sonuç ölçütlerinde istatistiksel olarak anlamlı bir
iyileşme bulundu. Grup 2 de iyileşme oranları istatistiksel olarak grup 1’den
daha yüksekti.Sonuç: Bu çalışmanın
sonunda boyun, bel ve diz ağrısı olan yaşlılarda fizik tedavi uygulamaları ağrı
ve yaşam kalitesi üzerine etkili olduğu saptanmıştır. Tüm vücut uygulanan
balneoterapi fizik tedavinin etkinliğini arttırabilir.
HİPERTANSİYON KONTROLÜNDE TAMAMLAYICI VE BÜTÜNLEŞİK (İNTEGRATİF) TEDAVİ ÖRNEĞİ: MASAJ
Authors: CEYHAN Özlem , ARSLAN Gürcan 
Published: May 2020

Hipertansiyon, dünya genelinde hem
gelişmiş hem de gelişmekte olan ülkelerde yaklaşık bir milyar insanı
etkilemekte olup kardiyovaküler hastalıklardan kaynaklanan ölümlerin en önemli
nedenlerinden biridir. Kan basıncı yüksekliği ile vasküler hastalıklar arasında
yüksek oranda ilişki olduğuna dikkat çeken çalışmalar gösteriyor ki, kan basıncında
meydana gelen küçük bir düşüş bile kardiyovasküler komplikasyonların (özellikle inme) önlenmesine katkıda bulunabilir.
Bu nedenle esansiyel hipertansiyonun önlenmesi ve etkin tedavisi büyük önem
taşımaktadır. Farmakolojik tedavinin kan basıncını kontrol altına almada ve
hipertansiyonu olan bireylerin yaşam kalitesini yükseltmede yeterli etkiyi
oluşturamadığı düşünülmekte, günden güne hasta sayısının artması ve kullanılan
antihipertansif ilaçların olumsuz etkileri nedeniyle bireyler farklı tedavi
yöntemleri arayışına girmektedir. Günümüzde
sıklıkla medikal tedaviyle birlikte tercih edilen tamamlayıcı ve bütünleşik
(integratif) tedaviler arasında olan yoga, akupunktur ve masaj gibi
iyileştirici ve yan etki düzeyi az olan yöntemler kronik hastalığı olan
bireyler tarafından kullanılmaktadır.

Tamamlayıcı ve bütünleşik (integratif) tedaviler
arasında yaygın olan yöntemlerden biri ise masajdır. Eisenberg et al.
tarafından yapılan çalışmada, tamamlayıcı ve bütünleşik (integratif) tedavi
yöntemlerine başvuran hastaların yaklaşık yarısı masaj uygulamasını tercih etmektedir.
Hipertansiyon, kardiyak cerrahi sonrası rehabilitasyon, baş ağrısı, demans, stres, fibromiyalji, osteoartrit, yorgunluk ve meme
kanseri gibi birçok kanser türünün semptomlarını hafifletmek ve yaşam
kalitesini artırmak için kullanılmaktadır.

Masajın, kan basıncı üzerine vücuttaki kan
akışını dengelemesi ve gevşeme cevabının bir sonucu olduğu yapılan meta-analiz
çalışmaları ile desteklenmektedir. Son yıllarda çok sayıda yapılan randomize
kontrollü çalışma kan basıncını kontrol altına almada destekleyici yöntem
olarak masajın kullanılmasının etkili olduğunu bildirilmiştir.

Bu makalede, masajın olumlu etkileri göz önüne
alındığında hipertansiyon yönetiminde nonfarmakolojik bir yöntem olarak
uygulanabileceği önerilmektedir.
Çocuklarda Akut,Komplike Apandisit ve Karın Ağrısı Gözlem Hastalarında Hemogram Parametreleri ve C-reaktif protein Değerlerinin Karşılaştırılması
Authors: DOĞAN Gül , İPEK Hülya , DEMİR Emre , AFŞARLAR Çağatay Evrim 
Published: May 2020

Amaç:Dünyada
akut karının yaygın nedenlerinden biri olarak kabul edilen apandisitin tanısı
özellikle çocukluk çağında zordur ve başka hastalıklarla karışabilmektedir.Çalışmamızdaakut,komplike
apandisit tanısı alan ve karın ağrısı nedeniyle klinik izleme alınan hastaların ilk yatışlarında alınan c-reaktif
protein (CRP) ve hemogram parametrelerinin analiz edilerek aralarındaki
ilişkinin ortaya konulması amaçlanmıştır.

Gereç
ve Yöntem: Ocak 2016-Mayıs 2017 tarihleri arasında hastanemize
karın ağrısı yakınması ile başvuran 573 çocuk hasta geriye dönük olarak
tarandı. Hastalar karın ağrısı nedeniyle takip edilenler,akut ve
komplikeapandisitolanlarolmak üzere üç gruba ayrıldı. Tüm hastaların demografik
verileri,CRP,hemogram parametreleri ve bundan türetilen oranlar kaydedildi.

Bulgular:
NLO, WBC/MPV, CRP/MPV, PLO ve MPV/L değerleri apendektomi yapılan hastalarda
karın ağrısı gözlem grubundan daha yüksek çıkmıştır (p&lt;0.001).Hb, MCV, MPV,
htc, platelet sayıları gruplara göre istatistiksel olarak anlamlı farklı
değildi (p&gt;0.05)

Sonuç:
NLO, WBC/MPV, CRP/MPV, PLO ve MPV/L değerlerinin apendektomi yapılan hastalarda
daha yüksek çıkması nedeniyle operasyona karar vermede yada karın ağrısı
gözlemleri belirlemede bu parametrelerin tanıyı destekleyeceği kanaatindeyiz.
Concurrent Bilateral Subtrochanteric Femoral Fracture Related with Long-Term Biphosphonate Usage
Authors: ÖZTÜRK Recep , ATALAY İsmail , ÖZANLAĞAN Emre , ATEŞ Ömer , EKŞİOĞLU Mehmet 
Published: May 2020

Bisphosphonates are widely
used in the treatment of osteoporosis.
Bisphosphonates act via suppression
of bone resorption.Growing numbers
of atypical femur fractures have been reported in the use of long-term
bisphosphonates in recent
years.In this case report, we present clinical
presentation, diagnosis,
and treatment as well as follow-up of a 60-year-old
postmenopausal female patient
who had metastatic
breast carcinoma, with bilateral femoral
subtrochanteric fracture associated
with long-term use of bisphosphonates.

Frozen species
were
sent from both
fracture lines, as
a result of
the frozen examination, no
tumoral tissue was found. Proximal
femoral nail to
right hiccup and
femur proximal anatomical plaque
to left hiccup
were
applied in the
same session. The patient was able
to walk with
full
load on both
lower extremities, and there
was no pain
in the hip
joint movements in
the rest and
exercise.
MORPHOMETRIC INVESTIGATION OF ANATOMIC STRUCTURES ON HUMERUS
Authors: YİLMAZ Seher , TOKPINAR Adem , TAŞTAN Mustafa , ATEŞ Şükrü , ÜNALMIŞ Demet , PATAT Dilara 
Published: May 2020

Giriş: Humerus; Skapula, radius ve ulna kemikleri arasında bulunur ve üst ekstremitenin en uzun ve en kalın kemiğidir. Bu çalışma, cerrahi işlemler, ortez ve protez uygulamaları, humerusta morfometrik ve antropometrik ölçümler için bir kriter olacaktır.Gereç ve yöntem: Bu çalışma, 80 (56 sol, 24 sağ) kuru humerus kemik örneklerinde 0.01 milimetre (mm) hassasiyetinde dijital kumpas kullanılarak yapıldı. Kemik örneklerinde yaş ve cinsiyet ayrımı yapılmadı. Humerus üzerinde sulcus intertubercularis uzunluğu, minimum gövde çapı, maksimum gövde çapı, kapitulum humer, troclea humeri gibi 21 parametre ölçüldü.Bulgular: Çalışmamızın sonuçlarına göre humerusun maksimum uzunluğu (HMU); solda ortalama 31,16 ± 2,44 mm ve sağda 29,85 ± 3,08 mm. Minimum gövde çapı (MinGW); Solda 17,62 ± 2,18 mm ve sağda 17,84 ± 1,82 mm’dir.Sonuç: Humerus kemiğinin normal anatomik yapısını bilmek ve bu bölgedeki cerrahi prosedürleri ve protez uygulamalarını yönlendirmek için bu kemikteki anatomik yapıların ortalama değerlerini hesaplamak çok önemlidir. Introduction: Humerus; It is located between the scapula, radius and ulna bones and is the longest and thickest bone of the upper extremity. This study will be a criterion for surgical procedures, orthosis and prosthesis applications, morphometric and anthropometric measurements on humerus.Materials and methods: This study was carried out using digital calipers with sensitivity of 0.01 millimeters (mm) on dry humerus bone specimens of 80 (56 left, 24 right). Age and sex were not differentiated in bone samples. Sulcus intertubercularis length, minimum trunk diameter, maximum trunk diameter, capitulum humer, troclea humer were measured on the humerus as 21 parameters.Results: According to the results of our study, the maximum length of the humerus (HMU); mean 31.16 ± 2.44 mm on the left and 29.85 ± 3.08 mm on the right. Minimum body diameter (MinGW); 17,62 ± 2,18 mm on the left and 17,84 ± 1,82 mm on the rightConclusion: It is very important to know the normal anatomical structure of the humerus bone and to calculate the average values ​​of the anatomical structures on this bone in order to guide the surgical procedures and prosthesis applications in this region.
Von Prag nach Krakau. Nachgedanken zum Alltag eines DAAD-Lektors
Authors: MEISTER Nick 
Published: May 2020

Die Reflexion des DAAD-Lektors über seine bisherige Tätigkeit an der Karlsuniversität in Prag sowie an der Pädagogischen Universität in Krakau bildet den Kern des Artikels. Er gibt nicht nur einen Einblick in seine Lehrtätigkeit, Stipendienwerbung und Kulturarbeit, sondern auch in die teils privaten, teils organisatorischen Umstände, unter denen er diese Aktivitäten ausübt. Der Autor legt den Schwerpunkt auf seine Eindrücke unddie damit einhergehenden wahrgenommenen Unterschiede im Übergang von der Sprachassistenz zum Lektorat. Eine Besonderheit spielt dabei die Erstbesetzung des Lektorats an der Pädagogischen Universität, die vor dem Hintergrund der polnischen Hochschulreform und der aktuellen Lage des DAAD in Polen vom Autor dargestellt wird.
Cascading Partial Rupture of the Flores Thrust during the 2018 Lombok Earthquake Sequence, Indonesia
Authors: Salman Rino , Lindsey Eric O. , Lythgoe Karen H. , Bradley Kyle , Muzli Muzli , Yun Sang-Ho , Chin Shi Tong , J. Tay Cheryl W. , Costa Fidel , Wei Shengji , Hill Emma M. 
Published: May 2020

AbstractA series of four Mw&gt;6 earthquakes struck the northern region of Lombok, eastern Indonesia, in a span of three weeks from late July to mid-August 2018. The series was thought to be associated with the Flores thrust, but the exact mechanism causing the unusual earthquake series has remained elusive. Our Interferometric Synthetic Aperture Radar analysis, combined with insights from seismology, indicates that the events originated at different hypocenter depths with differing fault geometries, which may explain the cascading behavior of the events, and indicates that better imaging of active fault geometry might provide some insight into future rupture behavior on other similar thrust systems. Our static stress change calculations suggest that the earlier events in the sequence played a role in promoting the later events. In addition, the second event brought the most significant impact on a nearby volcano, by causing volumetric expansion at its shallow magma plumbing system and unclamping its magma ascent zone, which may potentially have an impact on its future eruptive activity. However, no volcanic activity has so far occurred after the earthquakes. Finally, our damage proxy maps suggest that the second event caused the greatest damage to buildings.
Housing Planning for Informal Settlements: Pante-Macassar (East Timor)
Authors: Santos Ana , Virtudes Ana 
Published: May 2020

Regeneration of informal settlements is a topic being studied worldwide. Generally, cities comprise informal settlements, and consequently sustainability problems, requiring urgent actions. Often, the solutions regarding informal settlements do not adequately consider the local features, leading to difficulties such as environmental and public health deteriorations, weakening of housing access or troubles related to economic sustainability. Additionally, some housing programs don’t fit in community characteristics of social inequalities and poverty. In this sense, this paper intends to show the results of the development of a research methodology, to carry out strategies and to pursuit strategies of rethinking informal housing settlements, in an integrated and resilient way. It concludes with the application of a theoretical model to an informal neighbourhood in Pante Macassar, a city in the Oé-Cusse region of East Timor. The interest in this approach results from an ongoing Doctorate program in Civil Engineering at the University of Beira Interior, focused on the development of a set of good practice guidelines for the informal city regeneration. It aims to identify and assess informal and unhealthy features of urban settlements and their integration at city scale. The theoretical-conceptual issue addresses the informal settlements, comparing with formal housing programs.
Softened Variable Angle Truss Model (RA-STM): Model Description and Refinement/Optimization Proposals
Authors: Filho Benedito , Bernardo Luís , Horowitz Bernardo 
Published: May 2020

This article presents a recent softened truss model with variable angle, namely the refined RA-STM (Rotating-Angle Softened Truss Model), to model the behaviour of structural concrete plates under pure shear. The equations of the model, as well as the solution procedure, are summarized. Some predictions from the RA-STM are also presented, discussed and compared with experimental results available in the literature. It is shown that the refined RA-STM still needs to be refined. In addition, the need to generalize the RA-STM for more general loading cases is also discussed as well as the need to optimize the solution procedure in order to facilitate its computational implementation.