bluuebunny/crossref_metadata_2025_split huggingface dataset:
Role of MicroRNAs (224 and 96) as Novel Biomarkers for Hepatocellular Carcinoma
Authors: Mohamed Amal A. , El-Zawahry Mokhtar M. , Tantawi Omnia I. , Aalkhalegy Amyan , Fathalla Lamiaa Abdelfattah , Abo-Amer Yousry Esam-Eldin , Elmaghraby Mostafa Bedair , Elhoseeny Mohamed Mahmoud , El-Abgeegy Mohamed , Mostafa Sahar Mohamed , Abd-Elsalam Sherief , Badawy Ingy 
Published: November 2020

Background::
In the early stages of HCC, it is unsatisfactory to depend on alpha-fetoprotein for diagnosis.

Objective::
The current study evaluated the possibility of the two miRNAs which are miRNA-96 and miRNA-224 to act as biomarkers for HCC diagnosis.

Methods::
This study included 50 patients with HCV-induced HCC and 50 patients with HCV-induced liver cirrhosis for comparison as well as 67 healthy volunteers as controls. All participants were subjected to history taking, clinical examination, and laboratory investigations as well as quantification of serum miRNA-96 and miRNA-224 by real-time quantitative PCR.

Results::
MicroRNA 224 level was significantly higher in HCC than the other two groups and was significantly higher in liver cirrhosis than the control group. MicroRNA 96 level was higher in HCC than the control group and was higher in cirrhotic group than both HCC and control groups. However, it doesn’t reach the statistical significance level. The best cut-off value of microRNA 96 for detecting HCC was 3.414 with a sensitivity of 67% and a specificity of 67%, (p-value
<0.001). The best cut-off value of microRNA 224 for detecting HCC was 16.75 with a sensitivity of 88% and a specificity of 85% (p-value<0.001).

Conclusion::
miRNA-224 could serve as a biomarker for the HCC diagnosis.
An Update to Enterococcal Bacteremia: Epidemiology, Resistance, and
Outcome
Authors: Jafari Sirous , Abdollahi Alireza , Sabahi Marjan , Salehi Mohammadreza , Asadollahi-Amin Ali , Hasannezhad Malihe , Seifi Arash 
Published: November 2020

Background::
An increase in resistant gram-positive cocci, especially enterococci, requires an epidemiologic re-assay and its results may affect empirical treatments for these infections.

Objective::
In this study, we investigated the microbial epidemiology and resistance pattern of enterococcal bacteremia.

Methods::
This study was a cross-sectional study that investigates all cases of positive blood cultures with Enterococcus spp. at a tertiary referral colligates hospital in Tehran in 2018.

Results::
Enterococcus spp. was isolated from blood cultures of a total of 73 patients. The most patients were male 42 (57.7%). The mean age of the patients was 58.8 (±18.8) years. Hospital-acquired infection was the most type of infection involving enterococcal bacteremia (80.8%) comparing with community-acquired (6.7%) and health care-associated (12.3%). Renal failure and cancer were the most underlying disease in E. faecalis and E. faecium, respectively. Mortality for Vanco-mycin-resistant enterococci (VRE) was approximately two times more than the sensitive ones. Between the dead/alive groups, the following items were different significantly (P.Value<0.05): Vancomycin resistance for enterococcus isolated, immunodeficiency as underlying disease, Mechanical ventilation, hospitalization period, and the empiric regimen.

Conclusion::
Increased antibiotic-resistant strains, especially Vancomycin-resistant enterococci (VRE), pose a serious threat to the general public, especially hospitalized patients, and increase mortality. Surveillance of microorganisms and antimi-crobial resistance is a crucial part of an efficient health care system.
Comparative Analysis of miRNA Expressions in Different Developmental Stages of Echinococcus granulosus in Mono-Phasic and Di-Phasic Culture Systems
Authors: Mortezaei Seifollah , Afgar Ali , Sadeghi Balal , Mohammadi Mohammad Ali , Mousavi Seyed Mohammad , Harandi Majid Fasihi 
Published: November 2020

Background:
The dog tapeworm, Echinococcus granulosus, is a zoonotic parasite affecting
humans and livestock across the globe. Basic research on the molecular biology and genetics of
E. granulosus improves our understanding of the biology and potential drug targets in various developmental
stages of E. granulosus in both definitive and intermediate hosts. There has been increasing
interest in the identification of microRNAs in parasitic organisms. The purpose of the current
study was to compare the activity of a selected profile of miRNAs in different developmental
stages of E. granulosus.

Methods:
Different developmental stages of the parasite were obtained from ex vivo as well as in
vitro cultured E. granulosus. MicroRNAs were extracted from the ex vivo germinal layer and invaginated
protoscoleces as well as the in vitro generated microcysts, evaginated protoscoleces, and
strobilated worms. The expression of the selected miRNAs was evaluated by RT-qPCR for each
stage.

Results::
Four out of five miRNAs were present and active in different developmental stages of E.
granulosus. A significant over-expression of miR-61 was observed in the germinal layer and during
the protoscolex transformation into the microcysts, however, miR-10 was more expressed in
the mature strobilated forms than in the other stages. Let-7 and miR-3489 showed a high expression
in the germinal layer.

Conclusion:
Differential expression of four miRNAs among different in vitro and ex vivo developmental
stages of E. granulosus was documented in the present study. Further experimental investigations
are required to elucidate the probable role of the miRNAs in bi-directional differentiation
of protoscoleces either into the strobilated worm or to a secondary hydatid cyst and the potential of
these miRNAs as drug targets.
Serum Levels of miR-223-3p and miR-223-5p in Prostate Diseases
Authors: Dülgeroğlu Yakup , Eroğlu Onur 
Published: November 2020

Introduction:
Prostate Cancer (PCa) is the second most common cancer in males and the
fifth in cancer-associated mortality. Although the Prostate-Specific Antigen (PSA) test is widely used
in PCa screenings, it has significant limitations in the differential diagnosis of PCa. Therefore, studies
on developing new biomarkers for PCa diagnosis are ongoing. MiRNAs are good candidate biomarkers
for the diagnosis of cancers, including prostate cancer, as they can be easily detected from
circulation.

Objective:
In this study, it is aimed to determine the diagnostic value of serum levels of miR-223-3p
and -223-5p in Benign Prostate Hyperplasia (BPH), Chronic Prostatitis (CP) and Prostate Cancer
(PCa).

Methods:
Serum samples were collected from 68 patients in total (25 BPH, 10 CP, 33 PCa). MiR-223-
3p and -223-5p levels were measured in serum with qRT-PCR. The Ct values of miRNAs were normalized
according to the Ct value of ce-miR-39 and calculated -ΔCt values were used for statistical
analyses.

Results:
The serum levels of miR-223-3p and -223-5p were downregulated in the PCa and CP groups,
compared to the BPH group. There was no statistically significant difference between PCa and CP
groups. The sensitivity and specificity of miR-223-3p, -223-5p and their combination were calculated
as 88% and 88%; 86% and 79%; 93% and 92% in discriminating BPH and PCa groups, respectively.

Conclusion:
In this study, it was shown that miR-223-3p and -223-5p were both detectable in circulation.
miR-223-3p, -223-5p, and their combination may be good candidate biomarkers for prostate cancer
diagnosis. Also, observation of similar serum levels of miR-223-3p and -223-5p between CP and
PCa groups suggests that miR-223 may play a role in prostate cancer development originated from
chronic inflammation.
Predicted Influences of Artificial Intelligence on the Domains of Nursing: Scoping Review
Authors: Buchanan Christine , Howitt M Lyndsay , Wilson Rita , Booth Richard G , Risling Tracie , Bamford Megan 
Published: November 2020

Background
Artificial intelligence (AI) is set to transform the health system, yet little research to date has explored its influence on nurses—the largest group of health professionals. Furthermore, there has been little discussion on how AI will influence the experience of person-centered compassionate care for patients, families, and caregivers.

Objective
This review aims to summarize the extant literature on the emerging trends in health technologies powered by AI and their implications on the following domains of nursing: administration, clinical practice, policy, and research. This review summarizes the findings from 3 research questions, examining how these emerging trends might influence the roles and functions of nurses and compassionate nursing care over the next 10 years and beyond.

Methods
Using an established scoping review methodology, MEDLINE, CINAHL, EMBASE, PsycINFO, Cochrane Database of Systematic Reviews, Cochrane Central, Education Resources Information Center, Scopus, Web of Science, and ProQuest databases were searched. In addition to the electronic database searches, a targeted website search was performed to access relevant gray literature. Abstracts and full-text studies were independently screened by 2 reviewers using prespecified inclusion and exclusion criteria. Included articles focused on nursing and digital health technologies that incorporate AI. Data were charted using structured forms and narratively summarized.

Results
A total of 131 articles were retrieved from the scoping review for the 3 research questions that were the focus of this manuscript (118 from database sources and 13 from targeted websites). Emerging AI technologies discussed in the review included predictive analytics, smart homes, virtual health care assistants, and robots. The results indicated that AI has already begun to influence nursing roles, workflows, and the nurse-patient relationship. In general, robots are not viewed as replacements for nurses. There is a consensus that health technologies powered by AI may have the potential to enhance nursing practice. Consequently, nurses must proactively define how person-centered compassionate care will be preserved in the age of AI.

Conclusions
Nurses have a shared responsibility to influence decisions related to the integration of AI into the health system and to ensure that this change is introduced in a way that is ethical and aligns with core nursing values such as compassionate care. Furthermore, nurses must advocate for patient and nursing involvement in all aspects of the design, implementation, and evaluation of these technologies.

International Registered Report Identifier (IRRID)
RR2-10.2196/17490
Los procesos de gestión de documentos en la Ley General de Archivos de México: análisis de contenido desde una perspectiva cuantitativa
Authors: Bonal-Zazo José-Luis , Ortego-de-Lorenzo-Cáceres María-Pilar 
Published: November 2020

Se analizan las referencias sobre procesos de creación, captura y gestión de documentos en la Ley General de Archivos (LGA) de México mediante una metodología cuantitativa de análisis de contenido, la cual permite valorar, tanto de forma absoluta como ponderada, la presencia de seis grandes categorías de procesos en la LGA: Valoración y disposición; Conservación y preservación; Organización y descripción; Acceso y transparencia; Difusión, y, por último, Producción y seguimiento de documentos; así como el enfoque bajo el que es tratada cada una de las categorías (actividades, agentes o elementos). Mediante la metodología apuntada, se han identificado 347 referencias sobre las categorías señaladas, con un valor ponderado global de 301 puntos, la mayoría de los cuales corresponden a las categorías de Valoración y disposición (29.40 %); Conservación y preservación (26.58 %); Organización y descripción (18.44 %), y Acceso y transparencia (15.94 %); mientras que las dos categorías restantes presentan valores inferiores a 6 %. El análisis de los resultados permite concluir que en la LGA se conjugan procesos archivísticos tradicionales con procesos más novedosos, como trazabilidad, interoperabilidad, seguridad de información o gestión de metadatos.
Absorption removal of hydrogen sulfide from recirculated biogas
Authors: Gróf Nikolas , Barbušová Jana , Hencelová Kristína , Hutňan Miroslav 
Published: November 2020

Abstract
This study is focused on the effect of biogas recirculation with hydrogen sulfide removal on anaerobic treatment of sulfur-enriched synthetic wastewater in a UASB reactor. The presence of hydrogen sulfide in biogas causes problems in its further energy recovery while sulfides inhibit the anaerobic process. The reactor was gradually loaded with sulfates and their effect on the reactor operation was monitored. At the same time, external absorption of hydrogen sulfide from biogas with absorbent regeneration was operated. The results show that low concentrations of added sulfates support biogas production. At the sulfate concentration of 125 mg l−1, biogas production increased by approximately 2 l d−1. However, further increasing of the amount of sulfates in the substrate led to the opposite effect. At twice the amount of sulfates, the biogas production decrease by 1 l d−1 and its adverse effects on the removal of N-NH4, NC and P-PO4 were observed. Biogas recirculation through the absorption column ensured a decrease in the hydrogen sulfide concentration from 19 960 ppm to 4 030 ppm and an increase in the methane content from 59.2 % to 83 % and also a decrease in the sulfides concentration in the reactor. From the measured data it can be concluded that this method reduces sulfides inhibition.
Diagnostic and Prognostic Value of Glycated Hemoglobin (HbA1c) in Patients with Diabetes Mellitus and Thalassemia
Authors: Yordanov E. , Hristov E. , Parvova I. , Petkova V. , Andreevska K. 
Published: November 2020

Abstract
Diabetes mellitus (DM) is a metabolic disease characterized by hyperglycemia, which is caused by impaired insulin secretion, insulin action, or both. Four complications of DM have been identified – diabetes microangiopathy including nephropathy, retinopathy and polyneuropathy and diabetes macroangiopathy. DM is diagnosed on the basis of one of the following criteria: 1. Presence of typical symptoms – polyuria, polyphagia, polydipsia, weight loss and accidental plasma glucose value ≥ 11.1 mmol/l. 2. Fasting plasma glucose level ≥ 7.0 mmol/l. 3. Oral Glucose Tolerance Test (OGTT) – 2-hour blood glucose level ⩾ 11.1 mmol/l. 4. Test for glycated hemoglobin HbA1c⩾6.5%. Regular monitoring of blood glucose is crucial for good control of diabetes. Two are the most commonly available methods for glycemic monitoring – 1) home self-assessment; 2) HbA1c test. In this paper we discuss the diagnostic and prognostic value of HbA1c in comorbid patients with DM and diseases, where there are deviations in hemoglobin and erythrocyte values. In the scientific literature, single reports are available that give scarce guidance on how to manage these patients. It is mentioned that anemia should be treated individually, however no data could be found on what should be the standard behavior in such patients. According to the American Diabetes Association, only the blood glucose should be used when the relationship between HbA1c and glycemia is altered. Because of the diverse gene pool of the US population, different variants of hemoglobin may interfere with HbA1c measurements, although tests in the USA are standardized. Discrepancies between HbA1c and plasma glucose should suggest that testing for HbA1c may not be reliable for a particular patient. We present a clinical case of a comorbid patient with DM and thalassemia minor, whose glycemic control with glycated hemoglobin HbA1c is unreliable.
Primary Orbital Lymphoma – A Challenging Diagnosis
Authors: Vylkanov St. , Trifonova K. , Slaveykov K. , Dzhelebov D. 
Published: November 2020

Abstract

Background and purpose: The occurrence of primary orbital lymphoma comprises approximately 1% of non-Hodgkin’s lymphoma and 8% of extranodal lymphoma. The vast majority of orbital lymphomas are of B-cell origin, of which extranodal marginal zone B-cell lymphoma is the most common subtype. The purpose of this paper was to present the diagnostic challenges in a case of orbital lymphoma. Case presentation: An 84-year -old woman with orbital tumour was operated on after a long period of inappropriate treatment. It was later diagnosed as B-cell lymphoma.

Conclusion: Orbital lymphoma can be easily mistaken for another ocular disease due to the slowly progressing non-specific complaints of the patients. We should be alert to the possibility of this ocular diagnosis when we are presented with an elderly patient with proptosis.
What is Known About Emigration of the Romanian Doctors and What We Should Expect in the Future
Authors: Georgescu Mirela Cristina , Furtunescu Florentina Ligia , Frumusachi Otilia , Lopatica Lavinia , Mincă Dana Galieta 
Published: November 2020

Abstract
Romania has experienced in the last two decades a high emigration of the healthcare workforce, especially after joining the European Union. This study aimed to describe the trends in emigration of the Romanian health professionals and the potential changes of the intention to leave, considering the country context and the recent national measures. Four parameters intended to be analyzed: the number of physicians working in Romania, and in other countries respectively, the national capacity for providing undergraduate medical training and the intention to leave of the young graduates. We found an accelerate process of emigration of doctors, especially after the EU accession, wider compared to the increase of the national capacity for providing medical training and more rapid in the last years. The recent measures of motivation through the income increase appear to have limited impact on the intention to leave, at least for the young doctors. Systematic and coordinated efforts are needed in the next future to better understand which are the most effective interventions to respond to this situation. Among these interventions, improving the information system in this area should be a priority.
Topical Maintenance Treatments in Chronic Dermatitis
Authors: Rotaru Maria , Matran Irina Mihaela , Iancu Gabriela Mariana 
Published: November 2020

Abstract
The epidemiological importance of chronic dermatitis is constantly growing, this pathology being more and more common regardless of age, in most countries of the world. The pathogenesis of chronic dermatitis is complex and involves aeroallergens, immunological, dietary, climatic and psychosomatic factors. Due to the frequent recurrences but also the side effects of allopathic medicines, the number of patients with chronic dermatitis who opt for alternative treatments is increasing. In this paper we have identified allopathic topical treatments versus alternative topical treatments used in chronic dermatitis for which there is scientific evidence. The PubMed and Research Gate databases were analysed, the analysed period being 2007 – 2019. The search criteria were “chronic dermatitis”, “atopic dermatitis”, “psoriasis”, “alternative treatments”, “natural treatments”, “complementary treatments”,” treatments for chronic dermatitis”. We also analysed the mechanisms of action of drugs or active substances used as well as side effects secondary to allopathic and alternative therapies. New treatment options in atopic dermatitis, currently under evaluation, are topical inhibitors of phosphodiesterase and Janus kinase. For patients with chronic dermatitis who want alternative therapies to allopathic ones, there are products based on oat (Rhealba variety), camphor leaves, hydroxytyrosol, enzymatically interstered fats etc.
Two Great Intricate Gestational Syndromes. New Screening and Treatment Strategies
Authors: Gînfălean Georgeta 
Published: November 2020

Abstract
Gestational hypertension and premature birth frequently and independently affect the pregnancy prognosis with potentially detrimental influence of the pregnancy evolution. Pregnancy-induced hypertension, spectrum and categories of the systemic disease: gestational hypertension, preeclampsia, eclamsia, Hellp syndrome, overlapping preeclampsia (PE) chronic arterial hypertension, are some of the complications that can occur over the pregnancy evolution. Annually, preeclampsia affects approximately 4 million pregnancies. According to ACOG, preeclampsia complicates the evolution of pregnancies in a 2-8% ratio. According to WHO and FIGO, premature birth occurs after 37 weeks of complete amenorrhea (AS) (or 259 days of amenorrhea). A spontaneous premature birth complicates about 5-18% of pregnancies. I emphasize that the two gestational syndromes are intricate because 1/4-1/3 of premature births are associated with preeclampsia. Considered to be an important public health problem, it is important to identify new methods of evaluation, screening and therapy for gestational syndromes, taking into account the risk of increased recurrence of child birth in a subsequent pregnancy.
Access to nutrition information: a key to improving nutrition status among under-five children in farming households of Oyo State, Nigeria
Authors: Fadairo Anjolaoluwa Oreoluwa , Oyebode Lukman Abiodun , Amusat Adeniyi 
Published: November 2020

Abstract
Attaining adequate child nutrition requires prompt access to relevant nutrition information. Present information reveals that the nutrition status of children younger than five years is threatened. In this study we sought to assess under-five nutritional status among farming households in Oyo State, Nigeria. A multistage sampling procedure was used to sample a total of 146 mothers as respondents. Data were collected using interview schedule and were analysed using percentage, mean, Chi-square and Pearson Product Moment Correlation (PPMC). Most respondents were married (89.9%), currently working (93.2%) and had an average age, household size and monthly income of x¯ = 34.61, x¯ = 6 and ₦11,530, respectively. The respondents were mostly aware that cowpea is a major source of protein (x¯ = 0.99) and appropriate immunization of children is essential in aiding nutrition (x¯ = 0.99). They mostly sourced information on child nutrition from radio (x¯ = 0.87) and family and friends (x¯ = 0.87). Child nutritional status was high (59.6%). A significant relationship exists between educational attainment (χ2 = 10.781, P = 0.029), occupational status of the respondents (χ2 = 8.553, P = 0.014), awareness of adequate nutrition (r = 0.166, P = 0.044), source of information (r = 0.137, P = 0.010) and the child nutrition status. Improvements in nutrition campaign using available medical outlets and radio are advocated.
The influence of extraction method on the composition and analgesic activity of Calligonum comosum phenolic extracts
Authors: Chouikh Atef , Rebiai Abdelkrim 
Published: November 2020

Abstract
The aim of this study was to evaluate the analgesic activity and the effect of extraction methods (ultra-sound: UM and maceration: MM) and solvents (ethanol: EtOH and methanol: MeOH) on the composition of phenolic extracts from Calligonum comosum. The results obtained by HPLC analysis demonstrated that the ethanol extracts have shown the highest content of total phenolic and flavonoid compounds. Also, the presence of most known phenolic compounds has been identified in all extracts, especially in the MeOH UM extract. The HPLC analysis showed the presence of ascorbic acid in methanol extracts and caffeic acid in ethanol extracts, and the maceration method shows a high concentration of phenolic compounds, the vanillin was detected in MeOH UM and the appearance of chlorogenic acid in UM extracts, finally the emergence of gallic acid, quercetin and rutin in some extracts. According to the results of the analgesic power, the methanolic extract of the maceration method induces a significant decrease in abdominal cramps compared to the control group and the values obtained are very close from those obtained with the standard anti-inflammatory drug (indomethacin). This result confirmed the beneficial effect of this Saharan plant.