bluuebunny/crossref_metadata_2025_split huggingface dataset:
Silencing of MEG3 attenuated the role of lipopolysaccharides by modulating the miR-93-5p/PTEN pathway in Leydig cells
Authors: Zhou Xu , He Jingliang , Chen Jinbo , Cui Yu , Ou Zhenyu , Zu Xiongbing , Liu Nenghui 
Published: September 2020

Abstract
BackgroundLeydig cells reflect the activation of inflammation, decrease of androgen production, inhibition of cell growth and promotion of cell apoptosis under orchitis. Maternally expressed gene 3 (MEG3) exerts a crucial role in various human diseases, but under orchitis, the role and underlying molecular mechanism of MEG3 in Leydig cells remain unclear.MethodsLipofectamine 2000 was used for the cell transfections. qPCR and western blots assay were applied to assess the gene expression. ELISA assay was used to measure the TNFα, IL6 and testosterone secretion. CCK8 and EdU assay was employ to test the cell viability and proliferation respectively. Luciferase reporter and RIP assay were introduced to detect the binding of miR-93-5p with MEG3 and PTEN.ResultsLipopolysaccharides (LPS) induced TNFα and IL6 secretion, lowered testosterone production, inhibited cell viability and proliferation, and induced cell apoptosis in Leydig cells. MEG3 was upregulated in Leydig cells treated with LPS and that knockdown of MEG3 inhibited the role of LPS in Leydig cells. MEG3 absorbed miR-93-5p and that suppression of miR-93-5p restored the role of silenced MEG3 in Leydig cells under LPS treatment. miR-93-5p inhibited PTEN expression and that over-expressed PTEN alleviated the effect of miR-93-5p in Leydig cells treated with LPS. LPS activated the MEG3/miR-93-5p/PTEN signalling pathway in Leydig cells. ConclusionsThis study revealed that MEG3 serves as a molecular sponge to absorb miR-93-5p, thus leading to elevation of PTEN expression in Leydig cells under LPS treatment, offering a theoretical basis on which to establish potential new treatment strategies for orchitis.
Identiﬁcation, characterization and functional analysis of grape (Vitis vinifera L.) Mitochondrial Transcription Termination Factor (mTERF) genes in responding to biotic stress and exogenous phytohormone
Authors: Yin Xiangjing , Gao Yu , Song Shiren , Lu Jiang 
Published: September 2020

Abstract
Mitochondrial transcription termination factor (mTERF) is a large gene family which plays a significant role during plant growth against various environmental stresses. However, knowledge of mTERF genes in grapevine (Vitis L.) is limited. In this research, a comprehensive analysis of grape mTERF genes, including chromosome locations, phylogeny, protein motifs, gene structures, gene duplications, synteny analysis and expression profiles, was conducted. As a result, a total of 25 mTERF genes were identified from the grape genome, which are distributed on 13 chromosomes with diverse densities and segmental duplication events. The grape mTERF gene family is classified into nine clades based on phylogenetic analysis and structural characteristics. These VvmTERF genes showed differential expression patterns in response to multiple phytohormone treatments and biotic stresses, including treatments with abscisic acid and methyl jasmonate,and inoculation of Plasmopara viticola and Erysiphe necator These research findings, as the first of its kind in grapevine, will provide useful information for future development of new stress tolerant grape cultivars through genetic manipulation of VvmTERF genes.
Is the Correct Anatomical Placement of the Electrocardiogram (ECG) Electrodes Essential to Diagnosis in the Clinical Setting A Systematic Review
Authors: Hadjiantoni Alexandros Stavros 
Published: September 2020

Abstract
Background: Anatomical misplacement of the Electrocardiogram (ECG) electrode(s) is common, with significant impact on clinical diagnosis. Reasons are multi-faceted, with this review examining the consequential effects of misplacement to ECG morphology, diagnosis, prognosis, patient outcomes, and potential impact to patient care pathway. Objectives: This review examined the significance of misplacement, its’ commonality and ensuing effect on patient safety, accurate ECG acquisition and diagnosis, with evaluation of reasons for such misplacement.Methodology: Review of available literature was conducted using electronic databases. In-line with the Preferred Reporting Items for Systematic reviews and Meta – analysis protocols (PRISMA) 2015 checklist, this review was conducted with search criteria, search terms, eligibility for inclusion/ exclusion criteria, extraction and data analysis predetermined by the authors. Keywords were arranged according to grouping of terms surrounding ECG, anatomical placement, and diagnosis. The search strategy was conducted during September/October 2019. Scoping searches were conducted alongside reference lists of included studies hand searched (Snowballing) for further relevant studies. The Critical Appraisal Skills Programme (CASP) was used to methodically appraise papers (CASP, 2019). Screening of titles and abstracts of identified citations was performed by a single reviewer. Eligible articles then full text screened independently by two reviewers. Disagreements were discussed and resolved by a third reviewer. In instances of unclear reporting, authors were contacted to provide further information and clarity. Assessment of relevant literature and critical appraisal of primary research, pertaining to the clinical diagnosis and effects of anatomical misplacement of ECG electrodes, formulate the thematic discussion drawn by this review. Results: This review identified a plethora of causes, ranging from: operator error; lack of anatomical awareness; inaccurate assessment of anatomical landmarks; obesity; differences in anatomy/gender; levels of undress and lack of appreciation of consequences of misplacement, both modifiable and non-modifiable attributable to electrode misplacement. Clinical diagnosis can be altered owing to erroneous placement of electrodes. ECG morphology is altered due to incorrect anatomical misplacement, culminating substandard practice, a missed diagnosis or misdiagnosis and potential harm. Conclusion: Correct anatomical placement of ECG electrodes is essential to diagnosis in the clinical setting. Peer-led educational intervention with mandatory training is essential to improve practice. PROSPERO Registration Number: CRD42019152461
Necroptosis Triggered by ROS Accumulation and Ca2+ Overload, Partly Explains the Inflammatory Responses and Anti-Cancer Effects Associated With 1Hz, 100 mT ELF-MF in vivo
Authors: Barati Mojdeh , Javidi Mohammad Amin , Darvishi Behrad , Shariatpanahi Seyed Peyman , Moosavi Zahra S. Mesbah , Ghadirian Reyhane , Khani Tahereh , Sanati Hassan , Simaee Hossein , Barough Mahdieh Shokrollahi , Farahmand Leila , Ansari Alireza Madjid 
Published: September 2020

Abstract
Background: Focus on application of non-ionizing, extremely low frequency magnetic fields (ELF-EMF) as an alternative approach for treating cancer is rapidly rising nowadays. Nevertheless, little is known about the underlying anti-tumoral mechanism of action of them. Methods: In the present study, for the first time, we reported that along with apoptosis, 2 h/day exposure to 100 Hz, 1 mT ELF-EMF for a 5-day period, can induce necroptosis, a specific type of programed necrotic cell death, by promoting RIPK1/RIPK3/MLKL pathway which may also be responsible for observed pro-inflammatory responses in vivo, evident from an increase in plasma levels of pro-inflammatory cytokines including TNF-α, IL-1β, IL-2, IL-6, IL-17A and IFN-γ. Alongside, 30-day exposure to this system could also significantly suppress tumor growth and expression of markers of tumor cell proliferation, angiogenesis, and metastasis, namely Ki-67, CD31, VEGFR2 and MMP-9. Results: The number of tumor infiltrating lymphocytes (TILs), especially CD8+ Th cells were significantly increased following exposure to ELF-EMF. Interestingly, pretreating cancer cells with N-acetyl cysteine, a free-radical scavenger, or verapamil, an L-type calcium channel blocker in vitro, could diminish observed necroptotic and apoptotic responses while pretreating with calcium chloride, could aggravate responses.Conclusions: Overall, results of present study demonstrated that along with apoptosis, necroptosis is also a prominent form of cell death induced by exposure to ELF-EMF which is also dependent on elevated intracellular levels of ROS and calcium.
A Resting-State Functional MRI Study in Patients with Vestibular Migraine During Interictal Period
Authors: Wang Shuqing , Wang Haiping , Liu Xuejun , Yan Wenjing , Wang Minghui , Zhao Renliang 
Published: September 2020

Abstract
AimTo evaluate the spontaneous neuronal activities and the changes of brain functional network in patients with vestibular migraine (VM) using resting-state functional MRI (fMRI) during the interictal period.MethodsThree groups included 18 patients with VM, 21 patients with MWoA and 21 HCs underwent the scanning of the resting-state fMRI. First, brain regions with significant differentia of amplitude of low frequency fluctuation (ALFF) values were obtained. Secondly, functional connectivity (FC) analysis was performed in the brain region(s) with the most significant differentia of ALFF values which was defined as region of interest (ROI).Results(1) Compared with healthy volunteers, patients with VM and patients with MWoA showed significant ALFF decrease in the right putamen (P<0.05), and significant ALFF increase in the right lingual gyrus (P<0.05). What’s more, compared with patients with MWoA, patients with VM showed significant ALFF increase in the right lingual gyrus (P<0.05). In addition, we found that ALFF values in the right putamen of patients with VM were negatively correlated with the duration of migraine and the frequency of migraine attacks (P<0.05). (2) Compared with HCs, patients with VM showed significant FC increase among the cerebellum, the left dorsolateral superior frontal gyrus and the right putamen (P<0.05) but significant decrease among the left median cingulate, paracingulate gyri and the right putamen (P<0.05). Compared with patients with MWoA, patients with VM showed significant FC increase between the cerebellum and the right putamen (P<0.05) but significant FC decrease among the left median cingulate, paracingulate gyri and the right putamen (P<0.05).ConclusionThere are functional abnormalities in nociceptive, vestibular and visual cortex regions in patients with VM during the interictal period.
Inversion of Love Waves in Earthquake Ground Motion Records for Two-dimensional S-wave Velocity Model of Deep Sedimentary Layers
Authors: Kasamatsu Kentaro , Yamanaka Hiroaki , Sakai Shin’ichi 
Published: September 2020

Abstract
We have proposed a new waveform inversion method to estimate a 2D S-wave velocity structure of deep sedimentary layers using broadband Love waves. As a preprocessing operation in our inversion scheme, we decompose earthquake observation records into velocity waveforms at periods of 1 s interval. Then, we verify an assumption of 2D propagations of Love waves with polarization features based on a principal component analysis to select the segments applied for the inversion. A linearized iterative inversion analysis for the selected Love wave segments filtered at period of every 1 s allows a detailed estimation of boundary shapes of interfaces over the seismic bedrock with an S-wave velocity of approximately 3 km/s. We demonstrate the technique’s effectiveness with applications to observed seismograms in the Kanto plain, Japan. Differences between the estimated and existing structural models are remarkable at basin edges. A regional variation of the near-surface S-wave velocities in our model is similar to a distribution of surface geological classifications. Since a subsurface structure at a basin edge strongly affects earthquake ground motions in a basin with generations of surface waves, our method can provide a detail model of a complex S-wave velocity structure at an edge part for a strong ground motion prediction.
Understanding insulin and nutrition administration in glycemic control with the right side of the brain
Authors: Chase J. Geoffrey , Knopp Jennifer Launa 
Published: September 2020

Abstract
Background

Critically ill patients frequently experience stress-induced hyperglycaemia, leading to increased morbidity and mortality. Glycaemic control (GC) with insulin therapy alone has proven difficult, due to significant inter- and intra- patient variability in response to insulin therapy. This study reviews the problem and analyses the impact of physiological dynamics and patient variability on outcome glycemia.
Methods

A graphical model of metabolic dynamics is used to analyse the impact of fundamental glucose flux dynamics on insulin and nutrition administration in the context of maintaining a glycemic goal. It is used to delineate the limits of ability in controlling insulin and/or nutrition administration to achieve safe, effective glycemic control in critical illness in the presence of low insulin sensitivity and high insulin sensitivity variability.
Results

Insulin saturation limits insulin-mediated glucose uptake. At low insulin sensitivity, maintaining a glycemic target level requires reduced nutrition administration due to saturated insulin-mediated glucose uptake. Metabolic insulin sensitivity variability leads to insulin-mediated glucose uptake variability, requiring reduced nutrition administration at low insulin sensitivity and higher insulin doses to mitigate the risk of hypo- and hyper- glycemia.
Conclusions

This work reviews the clinical glycemic control problem using a graphically-based physiological analysis to show the need to control nutrition administration, along with insulin, to achieve safe, effective control. These reductions are necessary for highly insulin resistant patients, a condition typically occurring early in ICU stay. Glycemic control should directly control nutrition in addition to insulin to optimise all avenues of glucose flux and thus ensure safe, effective glycemic control.
In silico analysis of bacterial translation factors reveal distinct translation event specific pI values
Authors: JANA SOMA , Datta Partha Pratim 
Published: September 2020

Abstract
Background
Protein synthesis is a cellular process that takes place through the successive translation events within the ribosome with the help of the event-specific protein factors, namely, initiation, elongation, release, and recycling factors. The translation process is fundamental to all organisms living in the wide variety of environments. In this regard, we asked the questions about how similar are those translation factors to each other from a wide variety of bacteria? Hence, we did a thorough in silico study of the translation factors from 495 bacterial sp., and 4262 amino acid sequences, wherein we theoretically measured their pI and MW values that are the two determining factors for distinguishing individual proteins in 2D gel electrophoresis. Then we analyzed the output from various angles.
Results
Our study revealed that, not all the pI values are same or random, but there is a distinct order, such that the pI values of translation factors are translation event specific. We found that the translation initiation factors are mainly basic, whereas, elongation and release factors that interact with the inter-subunit space of the intact 70S ribosome during translation are strictly acidic. Further analysis revealed that the acidic property of those factors is due to the higher frequencies of glutamic acids. However, two translation factors, the translation initiation factor 2 (IF2) and the ribosome recycling factor (RRF) showed variable pI values. Remarkably, the variability of the pI values of these two factors showed distinct lineage with the order of phylogeny.
Conclusion
From our results we conclude that, among all the bacterial translation factors, elongation and release factors are more conserved in terms of their pI values in comparison to initiation and recycling factors. Acidic properties of these factors are independent of habitat, nature, or the phylogeny of the bacterial species. Furthermore; irrespective of the different shapes, sizes, and functions of the elongation and release factors, possession of their strictly acidic pI values indicate that the acidic nature of these factors is a necessary criterion, perhaps to interact into the partially enclosed rRNA rich inter-subunit space of the translating 70S ribosome.
Risk Factors for Venous Thromboembolism in Patients With Diabetes Undergoing Joint Arthroplasty
Authors: Deng Wei , Huo Lili , Yuan Qiang , Huang Deyong , Li Quan , Tian Wei 
Published: September 2020

Abstract
Background: Venous thromboembolism (VTE) is a significant complication after joint arthroplasty. Diabetes is related to a few changes in coagulation and fibrinolysis that may lead to thrombophilia. We aimed to investigate the incidence of and risk factors for VTEs in patients with diabetes undergoing total hip (THA) or total knee anthroplasty (TKA) in a single centre in China. Methods: Patients with diabetes who underwent THA or TKA from January 2016 to December 2018 (n=400) at Beijing Jishuitan hospital were recruited in this study. Lower limb venous Doppler ultrasound was performed before and after surgery to confirm deep venous thrombosis (DVT). Computer tomography pulmonary angiography was done to confirm pulmonary embolism (PE) for those with new postoperative DVT and typical symptoms of PE. A multivariate logistic regression model was conducted to examine factors associated with the development of postoperative VTE.Results: The overall incidence of postoperative VTE in patients with diabetes after THA or TKA was 46.8% (187 out of 400). Among the 187 VTE patients, 7.5% (14 out of 187) had proximal vein thrombosis and 92.5% (173 out of 187) had distal vein thrombosis. No PE occurred. Female patients and patients undergoing TKA had higher incidence of postoperative VTE. Patients who developed postoperative VTE were older, and had higher levels of preoperative D-Dimer level and Caprini score. Increased VTE risks were associated with high level of preoperative D-Dimer (OR=2.11, 95%CI=1.35-3.30) and TKA (OR=2.29, 95%CI=1.29-4.01). Postoperative initiation of concomitant mechanical prophylaxis and low molecular weight heparin (LMWH) was protective for postoperative VTE (OR=0.56, 95%CI=0.37-0.86). Conclusions: VTE is common in patients with diabetes undergoing joint arthroplasty. Patients undergoing TKA or with a high level of preoperative D-Dimer are at a considerable risk of developing postoperative VTE. There may be a protective role of postoperative initiation of concomitant mechanical prophylaxis and LMWH for VTE.
Relationship Between Leukocyte Counts and Large Vessel Occlusion in Acute Ischemic Stroke
Authors: Tárkányi Gábor , Karádi Zsófia Nozomi , Szabó Zsófia , Szegedi István , Csiba László , Szapáry László 
Published: September 2020

Abstract
Background: Neuroinflammation plays an important role in the pathogenesis of acute ischemic stroke (AIS) and peripheral leukocyte counts have proved to be independent predictors of stroke severity and outcomes. Clinical significance of large vessel occlusion (LVO) in AIS is increasing, as these patients are potential candidates for endovascular thrombectomy and likely to have worse outcomes if not treated urgently. The aim of our study was to assess the relationship between on admission leukocyte counts and the presence of LVO in the early phase of AIS.Methods: We have conducted a cross-sectional, observational study based on a registry of consecutive AIS patients admitted up to 4.5 hours after stroke onset. Blood samples were taken at admission and leukocyte counts were measured immediately. The presence of LVO was verified based on the computed tomography angiography scan on admission.Results: Total white blood cell (WBC) and neutrophil counts were significantly higher in patients with LVO than those without LVO (P<0.001 respectively). After adjustment for potential confounders total WBC counts (adjusted OR: 1.405 per 1x109/L increase, 95% CI: 1.209 to 1.632) and neutrophil counts (adjusted OR: 1.344 per 1x109/L increase, 95% CI: 1.155 to 1.564) were found to have the strongest associations with the presence of LVO. Total WBC and neutrophil counts had moderate ability to discriminate an LVO in AIS (AUC: 0.667 and 0.655 respectively). No differences were recorded in leukocyte counts according to the size of the occluded vessel and the status of collateral circulation in the anterior vascular territory. However, total WBC and neutrophil counts tended to be higher in patients with LVO in the posterior circulation (p= 0.005 and 0.010 respectively).Conclusion: Higher admission total WBC and neutrophil counts are strongly associated with the presence of LVO and has moderate ability to discriminate an LVO in AIS. Detailed evaluation of stroke-evoked inflammatory mechanisms and changes according to the presence of LVO demands further investigation.
Prevalence of Post Intensive Care Syndrome Among Japanese Intensive Care Unit Patients: A Prospective, Multicenter, Observational J-PICS Study
Authors: Kawakami Daisuke , Fujitani Shigeki , Morimoto Takeshi , Dote Hisashi , Takita Mumon , Takaba Akihiro , Hino Masaaki , Nakamura Michitaka , Irie Hiromasa , Adachi Tomohiro , Shibata Mami , Kataoka Jun , Korenaga Akira , Yamashita Tomoya , Okazaki Tomoya , Okumura Masatoshi , Tsunemitsu Takefumi 
Published: September 2020

Abstract
BackgroundMany studies have compared quality of life of post-intensive care syndrome (PICS) patients with age-matched population-based controls, instead of baseline quality of life. Furthermore, many studies on PICS used the 36-item Short Form (SF-36) health survey questionnaire version 2 but lack the data for SF-36 values before and after intensive care unit (ICU) admission. Thus, the clinically important changes in the parameters of SF-36 are unknown. Therefore, we determined the frequency of co-occurrence of PICS impairments at 6 months after ICU admission. We also evaluated the changes in SF-36 health survey questionnaire subscales and interpreted the patients’ subjective significance of impairment.MethodsA prospective, multicenter, observational cohort study was conducted in 16 ICUs across 14 Japanese hospitals. Adult ICU patients expected to receive mechanical ventilation for > 48 hours were enrolled and their 6-month outcome was assessed using the questionnaires. PICS definition was based on the physical status, change in SF-36 physical component score (PCS) ≥ 10 points; mental status, change in SF-36 mental component score ≥ 10 points; cognitive function, Short-Memory Questionnaire (SMQ) score worsened and SMQ score at 6 months < 40. Multivariate logistic regression model was used to identify the factors associated with PICS occurrence. The patients’ subjective significance of physical and mental symptoms was assessed using the 7-scale Global Assessment Rating.ResultsAmong 192 patients, 48 (29.6%) died at 6 months. Among the survivors at 6 months, 96 patients responded to the questionnaire; ≥1 PICS impairment occurred in 61 (63.5%) patients, and ≥ 2 occurred in 19 (19.9%) patients. Physical, mental, and cognitive impairments occurred in 33.3%, 14.6%, and 37.5% patients, respectively. Low education level was associated with PICS occurrence (Odds ratio: 3.8, 95% confidence interval: 1.1–17.9, P=0.036). Based on the patients’ subjective assessment, a 10-point change in PCS indicated moderate negative change.ConclusionsAmong the survivors who received mechanical ventilation, 64% had PICS at 6 months; co-occurrence of PICS impairments occurred in 20%. PICS occurrence was associated with low educational level. Future studies elucidating the minimal clinically important difference of SF-36 scores among the ICU patients and standardizing the PICS definition are required.Trial registrationUMIN000034072