bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of Effect of Handle Vibration Diesel-Fueled Single-Axle Tractor on Handgrip Strength of Operators
Authors: Busse Siraj , Sinclair Anthony , Wondimu Diresibachew , Reda Daniel 
Published: December 2020

Abstract
Background: The transmission of vibration from a single-axis tractor to the human body is determined by its dynamic response; this, in turn, depends on the physical characteristics of a person's hand, the contact area, the strength of the grip, the push force of the tractor, and the position. The purpose of this work was to measure and evaluate handgrip strength and fatigue resistance for operators of a 15 horsepower single-axle tractor before and after vibration exposure. Methods: Grip strength of dominant and non-dominant hands before and after 30 minutes of tilling operations was measured and recorded. The operators performed tilling tasks with the tractor in third gear, while the vibration levels were measured at the tractor handle along with the vertical, forward, and transverse directions. Results: The average operator grip strength was 33.6 ± 2.7 and 26.3 ± 3.3 kg and the average overall grip strength declined from 39.7 to 29.31 kg, although the average fatigue strength was 27.6 and 26.5 seconds for the dominant hand before and after vibration exposure. For the non-dominant hand, the mean grip strength measured was 28.7±2.9 and 23.1±1.9 kg and the maximum grip strength was 32.79 and 26.25, while fatigue was 29.76 and 22 seconds before and after tilling respectively.Conclusion: The average reduction in grip strength suggest considerable differences in grip strength for dominant and non-dominant hands of single axle tractor operators and shows that vibration transmitted from the single axle tractor handle has a major effect on the operators.
Anxiety and Facial Self-Contacts as Risk Factors for COVID-19 in Dental Practice.
Authors: Carrillo-Díaz María , Lacomba-Trejo Laura , Valle-Gonzalez Antonio del , Romero Martín , Gonzalez-Olmos María José 
Published: December 2020

Abstract
Background: This study makes innovative contributions regarding the association of facial self-contact frequency and duration in dental clinic patients with psychosocial factors such as dental fear, fear of COVID-19 and trait anxiety, because such self-contact could have an impact on the transmission of SARS-CoV-2.Results: Statistically significant associations were found between the number and duration of facial self-contacts with trait anxiety and dental fear. Women showed more frequent self-contact of the face in general and considering dental anxiety, we observe that the amount of self-contact for women remained. However, in men as dental fear increases, so do facial self-contacts. Trait anxiety is a mediating variable in the relationship between dental anxiety and the number of facial self-contacts.Conclusions: There is a positive association between the number of facial self-contacts and trait anxiety and dental anxiety. However, there is no association between self-contacts and fear of COVID-19. Women make a greater number of facial self-contacts, more trait anxiety and more dental anxiety. The relationship between dental anxiety and self-contacts was moderated by gender and the relationship between dental anxiety and self-contacts is mediated by trait anxiety.
Chronic lung diseases are associated with gene expression programs favoring SARS-CoV-2 entry and severity
Authors: Bui Linh , Winters Nichelle , Chung Mei-I , Joseph Chitra , Gutierrez Austin , Habermann Arun , Adams Taylor , Schupp Jonas , Poli Sergio , Peter Lance , Taylor Chase , Blackburn Jessica , Richmond Bradley , Nicholson Andrew , Rassl Doris , Wallace William , Rosas Ivan , Jenkins R , Kaminski Naftali , Kropski Jonathan , Banovich Nicholas 
Published: December 2020

Abstract
Patients with chronic lung disease (CLD) have an increased risk for severe coronavirus disease-19 (COVID-19) and poor outcomes. Here, we analyzed the transcriptomes of 605,904 single cells isolated from healthy and CLD lungs to identify molecular characteristics of lung cells that may account for worse COVID-19 outcomes in patients with chronic lung diseases. We observed a similar cellular distribution and relative expression of SARS-CoV-2 entry factors in control and CLD lungs. CLD epithelial cells expressed higher levels of genes linked directly to the efficiency of viral replication and innate immune response. Additionally, we identified basal differences in inflammatory gene expression programs that highlight how CLD alters the inflammatory microenvironment encountered upon viral exposure to the peripheral lung. Our study indicates that CLD is accompanied by changes in cell-type-specific gene expression programs that prime the lung epithelium for and influence the innate and adaptive immune responses to SARS-CoV-2 infection.
Delta-range coupling between prefrontal cortex and hippocampus supported by respiratory rhythmic input from the olfactory bulb in freely behaving rats
Authors: Mofleh Rola , Kocsis Bernat 
Published: December 2020

Abstract
An explosion of recent findings firmly demonstrated that brain activity and cognitive function in rodents and humans are modulated synchronously with nasal respiration. Rhythmic respiratory (RR) coupling of wide-spread forebrain activity was confirmed using advanced techniques, including current source density analysis, single unit firing, and phase modulation of local gamma activity, creating solid premise for investigating how higher networks use this mechanism in their communication. Here we show essential differences in the way prefrontal cortex (PFC) and hippocampus (HC) process the RR signal from the olfactory bulb (OB) allowing dynamic PFC-HC coupling utilizing this input. We used inter-regional coherences and their correlations in rats, breathing at low rate (~2 Hz) at rest, outside of the short sniffing bouts. We found strong and stable OB-PFC coherence, contrasting OB-HC coherence which was low but highly variable. PFC-HC coupling, however, primarily correlated with the latter, indicating that HC access to the PFC output is dynamically regulated by the responsiveness of HC to the common rhythmic drive. This pattern was present in both theta and non-theta states of waking, whereas PFC-HC communication appeared protected from RR synchronization in sleep states. The findings help to understand the mechanism of rhythmic modulation of non-olfactory cognitive processes by the on-going regular respiration, reported in rodents as well as humans. These mechanisms may also be important to understand how OB-pathology may lead to neurological consequences, similar to known olfactory disturbances in COVID-19.
The molecular mechanism of TiaoGanYiPi formula for treatment of chronic hepatitis B by network pharmacology and molecular docking verification
Authors: Cao Xu , Zao Xiaobin , Xue Baiquan , Chen Hening , Zhang Jiaxin , Li Shuo , Li Xiaobin , Zhu Shun , Guo Rui , Li Xiaoke , Ye Yongan 
Published: December 2020

Abstract
The Chinese herbal formula Tiao-Gan-Yi-Pi (TGYP) showed effective against Chronic Hepatitis B (CHB). In this study, we aimed to clarify the mechanisms and potential targets between TGYP and CHB through network pharmacology and molecular docking verification. The compounds of TGYP were identified in the TCMSP and CNKI databases, and their putative targets were predicted through SwissTargetPrediction and STITCH databases. The targets of CHB were obtained from the GeneCards, NCBI Gene, and DisGeNET databases. The above mentioned data were visualized using Cytoscape, and molecular docking showed the relationship between them. The expression of key targets was verified in GEO databases. Hence, we screened out 11 TGYP-related key targets for CHB included ABL1, CASP8, CCNA2, CCNB1, CDK4, CDKN1A, EP300, HIF1A, IGF1R, MAP2K1 and PGR. The key targets were predominantly enriched in the cancer, cell cycle and hepatitis B pathways and involved in the positive regulation of fibroblast proliferation, signal transduction, and negative regulation of gene expression biological processes, and expression of key target genes was related to HBV infection and liver inflammation. Through this newly constructed interaction network between TGYP and CHB, we identified active compounds and targets which could be further used for providing clinical guidance.
Hexaferrocenium Tri [Hexa(Isothiocyanato)Iron(III)] trihydroxonium Complex as a New DNA Intercalator&nbsp;for Electrochemical DNA Biosensor
Authors: Ariffin Eda , Zakariah Emma , Ruslin Farah , Kassim Muhammad , Yamin Bohari , Hasbullah Siti , Lee Heng 
Published: December 2020

Abstract
Ferrocene or ferrocenium has been widely studied in the field of organometallic complexes because of its stable thermodynamic, kinetic and redox properties. Novel Hexaferrocenium Tri[Hexa(Isothiocyanato)Iron (III)]Trihydroxonium (HexaFc) complex was the product from the reaction of ferrocene, maleic acid and ammonium thiocyanate and it was confirmed by elemental analysis CHNS, FTIR and single crystal x-ray crystallography. In this study, HexaFc was used for the first time as an electroactive indicator for DNA hybridisation biosensor. The UV-Vis DNA titrations with this compound showed hypochromism and redshift at 250 nm with increasing DNA concentrations. The binding constant (Kb) for HexaFc complex towards CT-DNA (calf-thymus DNA) was 3.1 × 104 M−1. These results indicated intercalator behaviour of the complex. To test the usefulness of this complex for DNA biosensor application, a porcine DNA biosensor was constructed. The recognition probes were covalently immobilised onto silica nanospheres (SiNS) via glutaraldehyde linker on a screen-printed electrode (SPE). After intercalation with the complex, the biosensor response to complementary porcine DNA was measured using differential pulse voltammetry. The DNA biosensor demonstrated a linear response range to the complementary porcine DNA from 1 × 10−6 to 1 × 10−3 µM (R2 = 0.9642) with a limit detection of 4.83 × 10−8 µM. The results indicated that HexaFc complex is a feasible indicator for DNA hybridisation evaluation without the need of chemical attachment as a label.
Morning chronotype decreases the risk of chemotherapy-induced peripheral neuropathy in women with breast cancer
Authors: Son Kyung-Lak , Jung Dooyoung , Lee Kwang-Min , Yeom Chan-Woo , Oh Kyu-Han , Kim Tae-Yong , Im Seock-Ah , Lee Kyung-Hun , Spiegel David , Hahm Bong-Jin 
Published: December 2020

Abstract
The purpose of this longitudinal prospective cohort study was to investigate the role of chronotype in the incidence of chemotherapy-induced peripheral neuropathy (CIPN) among women with breast cancer. A total of 128 subjects with breast cancer awaiting adjuvant chemotherapy without peripheral neuropathy participated in this study. The presence of CIPN was defined as a response of 3 or higher on a peripheral neuropathy subscale in the MD Anderson Symptom Inventory. Candidate psychiatric factors associated with CIPN were assessed, using the Composite Scale of Morningness, the Pittsburgh Sleep Quality Index, and the Hospital Anxiety and Depression Scale. To examine the association between chronotype and CIPN, we built logistic regression models, adjusting for demographic, clinical, and other psychiatric variables. Forty-nine participants received a chemotherapy regimen containing docetaxel, of which 29 (59%) developed CIPN. We performed subgroup analyses of docetaxel-treated participants. The morning chronotype was inversely associated with CIPN (odds ratio, 0.07; confidence interval, 0.01–0.48; p = 0.016) after adjusting for age, BMI, education, alcohol use, smoking, disease stage, sleep quality, depression, and anxiety. Our results suggest that the morning chronotype is a protective factor against the development of CIPN in patients with breast cancer who were treated with docetaxel.
Ab initio Study of the Structure, Elastic, and Electronic Properties of Ti3(Al1-nSin)C2 Layered Ternary Compounds
Authors: Ahams S. , Shaari A. , Ahmed Rashid , Pattah N. Abdul , Idris M. , Haq Bakhtiar 
Published: December 2020

Abstract
The MAX phase materials such as layered ternary carbides that simultaneously exhibit characteristics of metallic and ceramic materials have received substantial interest in recent years. Here, we present a systematic investigation of the electronic, structural stabilities, and elastic properties of Ti3(Al1-nSin)C2 (n = 0,1) MAX phase materials using the ab initio method via a plane-wave pseudopotential approach within generalized-gradient-approximations. The computed electronic band structures and projected density of states show that both Ti3SiC2 and Ti3AlC2 are metallic materials with a high density of states at the Fermi level emanating mainly from Ti-3d. Using the calculated elastic constants, the mechanical stability of the compounds was confirmed following the Born stability criteria for hexagonal structures. The Cauchy pressure and the Pugh’s ratio values establish the brittle nature of the Ti3SiC2 and Ti3AlC2 MAX phase materials. Due to their intriguing physical properties, these materials are expected to be suitable for applications such as thermal shock refractories and electrical contact coatings.
Catalytic Upgrading of Pyrolysis Bio-oil by Hydrotreating and Emissions Estimation of Combustion of Gas-phase Compounds&nbsp;
Authors: Doukeh Rami , Bombos Dorin , Bombos Mihaela , Oprescu Elena-Emilia , Dumitrascu Gheorghe , Vasilievici Gabriel , Calin Catalina 
Published: December 2020

Abstract
An attractive solution for improvement of pyrolysis bio-oil composition regarding the high content of oxygenated compounds is catalytic upgrading via hydrotreating process. The objective of this work is to evaluate the catalytic activity of CoMo /γ-Al2O3-HMS in bio-oil hydrotreating. The catalyst was characterized by surface area, X-ray diffraction (XRD), TEM, FT-IR and XPS analysis. The experiments were carried out in a flow fixed-bed reactor at the temperature range of 250-320 oC, pressure between 20-40 bar, and LHSV 3h-1. The results showed that increased temperature and pressure favors the hydrodeoxygenation process, reaching the maxim bio-oil conversion of 87.23%. Also, a chemical modelling algorithm was developed in order to evaluate the harmful emissions resulted from hydrotreating gaseous phase combustion. This calculation algorithm can be applied to any pressurized or atmospheric combustion engine fueled with different mixture of combustible gases such as: natural gas, hydrogen-enriched natural gas and oxy-fuel.
Non-Telecentric 2P microscopy for 3D random access mesoscale imaging at single cell resolution
Authors: Janiak Filip , Bartel Philipp , Bale Michael , Yoshimatsu Takeshi , Komulainen Emilia , Zhou Mingyi , Staras Kevin , Prieto-Godino Lucia , Euler Thomas , Maravall Miguel , Baden Tom 
Published: December 2020

Abstract
In neuroscience, diffraction limited two-photon (2P) microscopy is a cornerstone technique that permits minimally invasive optical monitoring of neuronal activity. However, most conventional 2P microscopes impose significant constraints on the size of the imaging field-of-view and the specific shape of the effective excitation volume, thus limiting the scope of biological questions that can be addressed and the information obtainable. Here, employing a non-telecentric (nTC) optical design, we present an ultra-low-cost, easily implemented and flexible solution to address these limitations, offering a several-fold expanded three-dimensional field of view that also maintains single-cell resolution. We show that this implementation also allows for straight-forward tailoring of the point-spread-function, increases effective excitation power, and achievable image brightness. Moreover, rapid laser-focus control via an electrically tunable lens allows near-simultaneous imaging of remote regions separated in three dimensions and permits the bending of imaging planes to follow natural curvatures in biological structures. Crucially, our core design is readily implemented (and reversed) within a matter of hours, and compatible with a wide range of existing 2P customizations, making it highly suitable as a base platform for further development. We demonstrate the application of our system for imaging neuronal activity in a variety of examples in zebrafish, mice and fruit flies.
Microstructure and Properties of Additively Manufactured Al-Ce-Mg Alloys
Authors: Sisco Kevin , Plotkowski Alex , Yang Ying , Leonard Donovan , Stump Benjamin , Nandwana Peeyush , Dehoff Ryan , Babu Suresh 
Published: December 2020

Abstract
Additive manufacturing of aluminum alloys is largely dominated by a near-eutectic Al-Si compositions, which are highly weldable, but have mechanical properties that are not competitive with conventional wrought Al alloys. In addition, there is a need for new Al alloys with improved high temperature properties and thermal stability for applications in the automotive and aerospace fields. In this work, we considered laser powder bed fusion additive manufacturing of two alloys in the Al-Ce-Mg system, designed as near-eutectic (Al-11Ce-7Mg) and hyper-eutectic (Al-15Ce-9Mg) compositions with respect to the binary L→Al+Al11Ce eutectic reaction. The addition of magnesium is used to promote solid solution strengthening. A custom laser scan pattern was used to reduce the formation of keyhole porosity, which was caused by excessive vaporization due to the high vapor pressure of magnesium. The microstructure and tensile mechanical properties of the alloys were characterized in the as-fabricated condition and following hot isostatic pressing. The two alloys exhibit significant variations in solidification structure morphology. These variations in non-equilibrium solidification structure were rationalized using a combination of thermodynamic and thermal modeling. Both alloys showed higher yield strength than AM Al-10Si-Mg for temperatures up to 350°C and better strength retention at elevated temperatures than additively manufactured Scalmaloy.
Parental Bonding Styles in Schizophrenia, Depressive and Bipolar Patients: A Comparative Study
Authors: Abbaspour Aidin , Bahreini Masoud , Akaberian Sherafat , Mirzaei Kamran 
Published: December 2020

Abstract
Background: Numerous bio-psychosocial factors play a role in the etiology of psychiatric disorders. In this regard, the relationship between parents and their children is significantly involved in developing the offspring mental health. However, there is no clear-cut answer as to which parental bonding style is more strongly associated with psychiatric diseases of patients. This study aimed to compare parental bonding styles in patients with schizophrenia, depression, and bipolar disorder in Bushehr province, Iran in 2018.Methods: In this cross-sectional comparative study, 130 patients with schizophrenia, depression, and bipolar disorder who referred to four outpatients psychiatric centers in Bushehr were selected using quota sampling. The patients were assessed and compared in terms of parental bonding styles. Data were collected using a valid and reliable parental bonding instrument (PBI). Data were analyzed using SPSS software (ver. 22), Chi-square and Kruskal-Wallis tests at a significant level of 0.05.Results: Results showed that the optimal parental bonding style (low control, high care) in bipolar disorder (43.05%), major depression (47.7%), and schizophrenia(38.5%) was the most prevalent style of parental bonding; however, 62.30% of the above patients suffered from inefficient paternal bonding styles and 51.53% from inefficient maternal bonding styles. Furthermore, the patients' maternal bonding styles were significantly different (p= 0.007) while their paternal bonding styles did not show any significant differences (p = 0.848).Conclusions: Most of the patients with psychiatric disorders were affected by ineffective parenting styles. The results also confirmed that despite the several bio-psycho-social factors involved in the development of psychiatric disorders, the crucial roles of parents, especially mothers, should not be ignored. It was further suggested that parents and parental bonding were important and fundamental factors for mental health promotion.
Direct RT-PCR amplification of SARS-CoV-2 from clinical samples using a concentrated viral Lysis-Amplification Buffer prepared with IGEPAL-630.
Authors: Castellanos-Gonzalez Alejandro , Shelite Thomas , Nicole Lloyd , Aygul Sadiqova , Ren Ping , Williams-Bouyer Natalie , Melby Peter , Travi Bruno 
Published: December 2020

Abstract
The pandemic of 2019 caused by the novel coronavirus (SARS-CoV-2) is still rapidly spreading worldwide. Nucleic acid amplification serves as the gold standard method for confirmation of COVID-19 infection. However, challenges faced for diagnostic laboratories from undeveloped countries includes shortage of kits and supplies to purify viral RNA. Therefore, it is urgent to validate alternative nucleic acid isolation methods for SARS-CoV-2. Our results demonstrate that a concentrated viral lysis amplification buffer (vLAB) prepared with the nonionic detergent IGEPAL enables qualitative detection of SARS-CoV-2 by direct Reverse Transcriptase-Polymerase Chain Reaction (dRT-PCR). Furthermore, vLAB was effective in inactivating SARS-CoV-2. Since this method is inexpensive and no RNA purification equipment or additional cDNA synthesis is required, this dRT-PCR with vLAB should be considered as an alternative method for COVID-19 detection.
Correlation of Sperm DNA Fragmentation Index With Seminal Plasma Biochemical Index and Semen Parameters in Infertile Men
Authors: Mao Xiao-Dong , Yang Xinyuan , Pan Feng , Liu Kang-Sheng 
Published: December 2020

Abstract
In general, routine semen analysis makes only limited predictions about a man's reproductive potential and is not always able to explain why he is infertile. In fact, many male infertility cases are caused by sperm DNA defects ,which routine semen quality analyses fail to detect. The relationship between sperm DFI, sperm parameters and their diagnostic value were analyzed and evaluated by observing the seminal parameters of infertile patients without accessory gonadal infection. Specimens of 151 cases were collected from infertile patients who visited the male department of STD and reproductive specialty clinic of our hospital from August 2018 to September 2019. SCD test was performed to measure the DNA fragmentation in native. The routine semen analysis was performed with a semen quality detection system (CFT-920, Jiangsu Ruiqi Life Science & Tech Dev .Co.Ltd) and supporting reagents. Seminal plasma malondialdehyde (MDA), and total antioxidant capacity (TAC) were assessed. Fructose(Fru) 、a-glucosidase (a-glu), and zinc (Zn) levels are quantitatively detected by kehua-310, a fully automated biochemical tester provided by Nanjing Xindibio. According to DFI level, there were 31 cases in group I (DFI ≤ 15%), 81 cases in group II (15% < DFI < 30%), and 39 cases in group III (DFI ≥ 30%). Compared with group II, there were significant differences in sperm survival rate, PR% and Fru by non-parametric test (Z = -2.16 -2. 43. − 2. 20,respectively,P < 0. 05). There were significant differences in sperm survival rate and PR% between group I and group III (t = 4. 32, 4.25, respectively, P < 0.01). Compared with group III, there were significant differences in sperm survival rate and PR% by non-parametric test (Z= -3. 26, -3. 50, respectively).Sperm DFI was negatively correlated with sperm survival rate and PR%(R=-0.56,-0.46,P < 0.01). DFI was positively correlated with MDA content (R = 0.42, P < 0.01) and negatively correlated with TAC (r=-0.40, P < 0.01).There was no correlation with age ,abstinence days, semen volume, sperm concentration, percentage of normal form sperm, Fru, a-Glu, Zn (R = 0. 15, 0. 05,0.03,-0.03, -0.2, -0.16,- 0.20, 0.03, 0.15, p > 0.05).The survival rate and PR% of sperm decreased significantly with the increase of DFI level, antioxidant can decrease the rate of DNA fragmentation, antioxidant can decrease the rate of DNA fragmentation. Semen volume and sperm concentration were mainly related to the secretion volume of accessory gonads and total sperm count, but no correlation was found between them and DFI.