bluuebunny/crossref_metadata_2025_split huggingface dataset:
Thrombin-activated platelet rich plasma (PRP) enhanced osteogenic differentiation of bone marrow mesenchymal stem cells by activing autophagy through miR-140-3p/SPRED2 axis
Authors: Jiang Shuting , Liu Hongyan , Zhu Weiyan , Yan Hui , Yan Beizhan 
Published: December 2020

Abstract
Background Mesenchymal stem cells transplantation gradually become a potential treatment for bone defect in clinic practice. This study aimed to investigate the molecular mechanism of PRP and autophagy for osteogenic differentiation in bone marrow mesenchymal stem cells (BMSCs). Methods Thrombin activated PRP was prepared and the BMSCs were treated with activated PRP with different concentration and transfected with miR-140-3p vector (mimics or inhibitor), si-SPRED2 or co-transfected with miR-140-3p inhibitor and si-SPRED2, respectively. qRT-PCR and Western blotting were used to determine the mRNA expression and protein expression. A luciferase reporter assay was conducted to identified the targeting relationship between iR-140-3p and SPRED2 Subsequently, cell proliferation was detected by MTT and ALP activity was also determined. Alizarin red staining was used for the evaluating the formation of calcium nodules. Results MiR-140-3p expression was found to be inhibited by PRP in a dose-dependent manner, besides, cell proliferation, ALP activity, the expression of COL-I, OPN, Runx2 and OCN, and the formation of calcium nodules related to osteogenic differentiation were enhanced by PRP. Subsequently, we found that PRP activated autophagy and up-regulated SPRED2 expression in BMSCs through suppressing miR-140-3p expression. Moreover, we confirmed that miR-140-3p targeted SPRED2 and negatively regulation its expression. Finally, the findings showed that inhibition of miR-140-3p enhanced cell proliferation, osteogenic differentiation and autophagy of BMSCs by negatively regulating SPRED2 expression. Conclusion Thrombin activated PRP accelerated osteogenic differentiation of BMSCs by activing autophagy through miR-140-3p/SPRED2 axis.
CD157 mediates mitochondrial production and transfer from bone marrow mesenchymal stem cells (BMSCs) to improve neuronal apoptosis and functional recovery after spinal cord injury
Authors: Li Jing , Li Heyangzi , Cai Simin , Bai Shi , Cai Huabo , Zhang Xiaoming 
Published: December 2020

Abstract
Background: Recent studies demonstrated that autologous mitochondria derived from bone marrow mesenchymal stem cells (BMSCs) might be valuable in the treatment of spinal cord injury (SCI). However, the mechanisms of mitochondrial transfer from BMSCs to injured neurons are not fully understood. Methods: We modified BMSCs by CD-157, a cell surface molecule as a potential regulator mitochondria transfer, then transplanted to SCI rats and co-cultured with OGD injured VSC4.1 motor neuron. We detected extracellular mitochondrial particles derived from BMSCs by transmission electron microscope and measured the CD157/cyclic ADP-ribose signaling pathway related protein expression by immunohistochemistry and Western blotting assay. The CD157 ADPR-cyclase activity and Fluo-4 AM was used to detect the Ca2+ signal. All data were expressed as mean ± SEM. Statistical analysis was analyzed by GraphPad Prism 6 software. Unpaired t-test was used for the analysis of two groups. Multiple comparisons were evaluated by one-way ANOVA or two-way ANOVA.Results: CD157 on BMSCs was upregulated when co-cultured with injured VSC4.1 motor neurons. Upregulation of CD157 on BMSCs could raise the transfer extracellular mitochondria particles to VSC4.1 motor neurons, gradually regenerate the axon of VSC4.1 motor neuron and reduce the cell apoptosis. Transplantation of CD157 modified BMSCs at the injured sites could significantly improve the functional recovery, axon regeneration and neuron apoptosis in SCI rats. The level of Ca2+ in CD157 modified BMSCs dramatically increased and when objected to high concentration cADPR, ATP content and MMP of BMSCs also increased.Conclusion: This study evidences that CD157 can regulate the produce and transfer of BMSCs-derived extracellular mitochondrial particles, enriching the mechanism of the extracellular mitochondrial transfer in BMSCs transplantation and providing a novel strategy to improve the stem cell treatment on SCI.
Niche Partitioning in the&nbsp;Rimicaris&nbsp;Exoculata Holobiont: The Case of the First Symbiotic Zetaproteobacteria
Authors: Cambon-Bonavita Marie-Anne , Aubé Johanne , Cueff-Gauchard Valérie , Reveillaud Julie 
Published: December 2020

Abstract
Background Mutualistic symbioses between invertebrate animals and chemosynthetic bacteria are the basis of life in hydrothermal vent ecosystems. The shrimp Rimicaris exoculata , which dominates animal communities along the Mid-Atlantic Ridge, houses a complex bacterial community in its enlarged cephalothorax, including the dominant Campylobacteria , Desulfobulbia (formerly Deltaproteobacteria ), Alpha proteobacteria , Gammaproteobacteria and some recently discovered iron oxyhydroxide-coated Zetaproteobacteria . This epibiotic consortium uses iron, sulfide, methane, and hydrogen as energy sources. Here, we generated shotgun metagenomes from Rimicaris exoculata cephalothoracic epibiotic communities to reconstruct and investigate symbiotic genomes. We collected specimens in three geochemically contrasted vent fields, TAG, Rainbow, and Snake Pit, to unravel the specificity, variability, and adaptation of Rimicaris –microbe associations. Results Our data enabled us to reconstruct 49 metagenome-assembled genomes (MAGs) from the TAG and Rainbow vent fields, including 16 with more than 90% completion and less than 5% contamination based on single copy core genes. These MAGs belonged to the dominant Campylobacteria , Desulfobulbia , Thiotrichaceae as well as some novel candidate phyla radiation (CPR) lineages. In addition, most importantly, two MAGs in our collection were affiliated to Zetaproteobacteria and had no close relatives (average nucleotide identity ANI < 77% with the closest relative Ghiorsea bivora isolated from TAG, and 88% with each other), suggesting potential novel species. Genes for Calvin-Benson Bassham (CBB) carbon fixation, iron, and sulfur oxidation, as well as nitrate reduction, occurred in both MAGs. However, genes for hydrogen oxidation and multicopper oxidases occurred in one MAG only, suggesting shared and specific potential functions for these two novel Zetaproteobacteria symbiotic lineages. Overall, we observed highly similar symbionts co-existing in a single shrimp at both the basaltic TAG and ultramafic Rainbow vent sites. Nevertheless, further examination of the seeming functional redundancy among these epibionts revealed important differences. Conclusion These data highlight microniche partitioning in the Rimicaris holobiont and support recent studies showing that functional diversity enables multiple symbiont strains to coexist in animals colonizing hydrothermal vents.
Clearance of Circulating Bacteria and Recovery of Microbiota Diversity Following Adoptive Cellular Therapy in Advanced Colorectal Cancer Patients
Authors: Yang Duo , Wang Xiaoli , Zhou Xinna , Wang Shuo , Zhao Jing , Yang Huabing , Morse Michael A. , Wu Jiangping , Yuan Yanhua , Li Sha , Hobeika Amy , Ren Jun , Lyerly Herbert Kim 
Published: December 2020

Abstract
Objective: There is increasing evidence of a human blood-microbiome that may have an immune modulatory effect. Anticancer therapies including adoptive cellular therapy (ACT) may influence this blood microbiome. The purpose of this study was to investigate impacts of biological ACT infusions on the blood microbiota. Materials and Methods: Plasma was collected from colorectal cancer patients (CRC) treated with either chemotherapy alone (oxaliplatin and capecitabine) (XELOX alone group, CT, n=19), or a mixed dendritic cell/cytokine induced killer cell product (DC-CIK) + XELOX (group, ICT, n=20). Circulating microbiota analysis was performed by amplifying and sequencing variable regions V3~V4 of bacterial 16S rRNA genes. The association of microbiota with clinical immunotherapeutic response was evaluated. Results: The plasma bacterial DNA copy number decreased and diversity of plasma microbiota significantly increased after administration of the DC-CIK product. The plasma bacterial DNA copy number correlated with the CD3-/CD16+/CD56+ NK cells in circulation. The diversity index was associated with CD3-/CD16+/CD56+ and CD8+/CD28+ cells infused. A more diverse blood microbiome including bifidobacterium, lactobacillus and enterococcus was identified among responders to immunotherapy compared with non-responders.Conclusions: Cellular immunotherapy can affect the plasma microbiota’s diversity in a manner favorable to clinical responses. Sustainable bacteria diversity was associated with benefit from immune therapy in CRC patients.
Physicochemical and Antibacterial Properties of Bivalent Freeze and Furnace Dried Nanoscale Cerium Oxide
Authors: Iqbal Neelam , Anastasiou Antonios , Aslam Zabeada , Raif Mostafa El , Do Thuy , Giannoudis Peter , Jha Animesh 
Published: December 2020

Abstract
Bone healing is a complex process, and if not managed, can lead to bacterial infections, non-union and compromised healing of bone. The broad overuse of antibiotics has led to the emergence of antibiotic-resistant bacteria. Due to the growing urgency to minimise the dependency on antibiotic drugs, alternative treatment strategies, including the use of nanoparticles, have attracted significant attention. Bivalent cerium oxide nanoparticles (Ce4+ and Ce3+) synthesised via a hydroxide mediated approach were calcined at 280, 385 and 815 ˚C identified using the Simultaneous Thermal Analysis technique. The resulting nanoparticles were characterised using X-ray Powder Diffraction, Fourier Transform Infrared Spectroscopy, Ultraviolet-Visible Spectroscopy, Transmission Electron Microscopy, and Electron Energy Loss Spectroscopy. The antibacterial potential of cerium oxide nanoparticles corresponds to the particle size and the presence of oxygen vacancies in the fluorite crystal structure. The antibacterial efficacy of nanoparticles was characterised at concentrations of 50, 100 and 200 µg/ml and tested against the following strains, Escherichia coli, Staphylococcus epidermis and Pseudomonas aeruginosa by determining the half-maximal inhibitory concentration (IC50). The calcination temperature was found to affect the agglomeration tendency, particle size distribution and the ratio of Ce3+:Ce4+ oxidation states. The hydroxide mediated approach yielded spherical nanoparticles of ceria with particles size ranging from 4 nm to 53 nm. The freeze-dried bivalent nanoparticles exhibited 18.5 ± 1.2%, 10.5 ± 4.4%, and 13.8 ± 5.8% increased antibacterial efficacy against Escherichia coli, Pseudomonas aeruginosa and Staphylococcus epidermis, respectively compared to nanoparticles consisting solely of Ce4+ ions, i.e. nanoparticles calcined at 815 ˚C.
Workload Indicators of Staffing Need Method in Determining Anesthesia and Surgical Technologist Staff in Operating Room
Authors: Khodayari-Zarnaq Rahim , Maleki Reza Aghebati , Mafakheri Zahra , Bozorghpour Niosha , Ezzati Esmaeil , Bahreini Rona 
Published: December 2020

Abstract
Background: Human resources in health are limited, and a variety of healthcare services are increasing, especially in developing countries. This study aimed to determine the anesthesia and surgical technologist staff for operating room based on the Workload Indicators of Staffing Need (WISN).Methods: This cross-sectional descriptive study was conducted at Shahid Madani Medical and Training Hospital in 2019. The data collected through interview and hospital documents observation. The WISN method was used to analyze and determine staffing need.Results: The WISN ratio for anesthesia and surgical technologist staff were 1.60 and 4.94, respectively. In other words, in both categories, this ratio is >1.00. Conclusions: WISN results can used to assess staffing capacity and capability over the previous year and the policy and financial implications due to staff shortages or surplus. As the workload of staff is an important issue and the staff have a right to have a standard workload in their position, then determining this workload is an essential issue in healthcare organization. Also, WISN method work as a human resource management tools, it improvers health managers to make right decision about staffing needs and then better manage of organization’s resources.
Japan’s development cooperation for health in Vietnam: A first holistic assessment on Japan’s ODA and non-ODA public resources cooperation
Authors: Lee Sangnim , Tachimori Hisateru , Sugai Maaya Kita , Ishizuka Aya , Uechi Manami , Akashi Hidechika , Hinoshita Eiji , Miyata Hiroaki , Shibuya Kenji 
Published: December 2020

Abstract
Background
To achieve Universal Health Coverage (UHC), which is the core driver of health Sustainable Development Goals (SDGs), by 2030, Japan strives to strengthen its development cooperation by mobilizing various resources. However, the involvement and roles of various actors from Japan in development cooperation for health have not been revealed across various entities of the Japanese government or to the public. This study is the first to systematically assess the overall picture of Japan’s publicly funded cooperation mobilizing Official Development Assistance (ODA) and non-ODA public resources to improve people’s health in Vietnam. A cross-sectional analysis of Japanese publicly funded projects implemented for health cooperation in Vietnam during December 2016 was conducted. A framework based on the six building blocks of health system defined by the World Health Organization was adopted in order to assess the target projects.
Results
Overall, 68 projects implemented through Japanese public funding were included in the final analysis. These 68 projects under 15 types of schemes were managed by seven different scheme-operating organizations and funded by five ministries. Forty-four of these (64.7%) were ODA and 24 (35.3%) were non-ODA projects. Among the recategorized six building blocks of the health system, the focus area of the largest proportion of projects was health service delivery (44%), followed by health workforces (25%) and health information systems (15%). Almost half the projects were implemented together with the central hospitals as Vietnamese counterparts. Only 10% projects were conducted at the local level. This study was unable to capture the synergetic effects of potential collaboration or harmonization among Japanese funded projects.
Conclusions
Several Japanese-funded projects addressed a wide range of health issues across all six building blocks of the health system in Vietnam. However, there is room for improvement in developing coordination and harmonization among the Japanese projects that are diversifying. Furthermore, establishing a target-country-specific mechanism for strategic coordination across Japanese ministries’ schemes can yield efficient and effective development cooperation for health. Our analytic approach using the recategorized six building blocks contributes to a more comprehensive understanding of efforts on strengthening the health system.
Costing a population health management approach for participant recruitment to a diabetes prevention study
Authors: Michaud Tzeyu L. , Wilson Kathryn , Silva Fabiana , Almeida Fabio , Katula Jeff , Estabrooks Paul 
Published: December 2020

Abstract
Background

Limited research has reported the economic feasibility—from both a research and practice perspective—of efforts to recruit and enroll an intended audience in evidence-based approaches for disease prevention. This study aims to retrospectively assess and estimate the costs of a population health management (PHM) approach to identify, engage, and enroll patients in a Type 1 Hybrid Effectiveness-Implementation (HEI), diabetes-prevention trial.
Methods

We used descriptive analyses and activity-based costing to estimate the recruitment costs of a PHM approach integrated within a HEI trial. Measures included total costs and costs per participant screened and enrolled to ensure trial accrual rates were achieved. We took the perspective of a healthcare system which may adopt, and possibly sustain, the strategy in the typical practice. We also estimated replication costs based on how the strategy could be applied in healthcare systems interested in referring patients to a local diabetes prevention program from a payer perspective. In this case, we examined differences in cost based on requirements for and types of, glucose testing, and by labor activities and non-labor resources associated with recruitment using sensitivity analyses.
Results

The total recruitment and enrollment costs were $389,949 to accrue 599 participants over approximately 15 months. The average cost per screened and enrolled participant was $276 and $651, respectively. Primary costs included hemoglobin A1c testing ($83,106), recruitment screening calls ($48,791), and personnel time ($96,187). Translating to the typical settings, total recruitment costs for replication was estimated as $192,625 (range: $42,523-$208,876). Sensitivity analysis results indicated replication costs would be approximately $229 - $430 per patient enrolled if glucose testing was necessary, based on the Medicare covered services. For a private payer perspective, and without glucose testing, per-participant screening and enrollment costs were estimated at $30.
Conclusions

A PHM approach can be used to accrue a large number of participants in a short period of time for an HEI trial, at a comparable cost per participant. However, when trial requirements are removed and PHM approaches are used as a method for healthcare systems to identify and enroll patients in a local program—the costs are relatively low.
The OsOXO2, OsOXO3 and OsOXO4 positively regulate panicle blast resistance in rice
Authors: Dong Jingfang , Zhou Lian , Feng Aiqing , Zhang Shaohong , Fu Hua , Chen Luo , Zhao Junliang , Yang Tifeng , Yang Wu , Ma Yamei , Wang Jian , Zhu Xiaoyuan , Liu Qing , Liu Bin 
Published: December 2020

Abstract
Background
Although panicle blast is more destructive to yield loss than leaf blast in rice, the cloned genes that function in panicle blast resistance is still very limited and the molecular mechanisms underlying panicle blast resistance remain largely unknown.
Results
In the present study, we have confirmed that the three OXO genes, OsOXO2, OsOXO3 and OsOXO4 from a blast-resistant cultivar BC10 function in panicle blast resistance in rice. The expression of OsOXO2, OsOXO3 and OsOXO4 were induced by panicle blast inoculation. Subcellular localization analysis revealed that the three OXO genes are all localized in the cell wall. Simultaneous silencing of OsOXO2, OsOXO3 and OsOXO4 decreased rice resistance to panicle blast, whereas the OsOXO2, OsOXO3 and OsOXO4 overexpression rice plants showed enhanced panicle blast resistance. More H2O2 and higher expression levels of PR genes were observed in the overexpressing plants than in the control plants, while the gene silencing plants exhibited less H2O2 and lower expression levels of PR genes compared to the control plants. Moreover, phytohormone treatment and the gene expression analysis showed that panicle blast resistance mediated by the three OXO genes was associated with the induction of JA and ABA signaling pathways but suppression of SA signaling pathway.
Conclusion
OsOXO2, OsOXO3 and OsOXO4 positively regulate panicle blast resistance in rice. The OXO genes could modulate the accumulation of H2O2 and expression levels of PR gene in plants. Moreover, the OXO genes mediated panicle blast resistance is associated with the activation of JA and ABA signaling pathways but suppression of the SA signaling pathway.
Factors associated with awareness, treatment and control of hypertension among 3,579 hypertensive adults in China: data from the China Health and Nutrition Survey
Authors: Wei Junxiang , Mi Yang , Li Yan , Xin Bo , Wang Youfa 
Published: December 2020

Abstract
Background: The prevalence of hypertension is high and rising in China, but most people with hypertension do not have their blood pressure under control. This study investigated hypertension awareness, treatment, and control and their associated factors among Chinese adults. Methods: Data collected from the 2011 China Health and Nutrition Survey (CHNS) from 12,991 Chinese adults were used. Hypertension was defined as systolic blood pressure ≥ 140 mm Hg, diastolic blood pressure ≥ 90 mm Hg, self-reported prior diagnosed hypertension, or taking antihypertensive medications. Hypertension awareness, treatment, and control were defined as a self-reported diagnosis of hypertension, current use of antihypertensive medication, and blood pressure < 140/90 mm Hg, respectively. Multivariate logistic regression was performed to examine factors associated with hypertension awareness, treatment, and control. Results: Overall, 3,579 (27.6%) of the CHNS adult participants had hypertension, of whom 55.7% were aware of their diagnosis, 46.5% were treated with antihypertensive medications, but only 20.3% had their blood pressure under control. Higher hypertension treatment was associated with older age (OR=2.57; 95%CI, 1.65-4.02), urban residency (1.50; 1.14-1.97), living in the Eastern region (1.52; 1.14-2.01), and being overweight/obese (1.99; 1.39-2.84). Hypertension awareness was associated with similar factors as hypertension treatment but was also associated with being female (1.37, 1.12-1.66). Poor hypertension control was associated with being overweight/obese (0.56; 0.42-0.76) and minority ethnicity (0.52; 0.31-0.86). ﻿Conclusion: Hypertension is a major public health challenge in China. The prevalence of hypertension awareness, treatment, and control are still low despite existing public health policies and programs to reduce the burden of hypertension. More intensive hypertension screening and treatment programs are warranted in China.