bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Value and Clinical Significance of ZNF582 gene Methylation in the Diagnosis of Cervical Cancer
Authors: Zhang Chunhe , Fu Shaowei , Wang Luyue , Wang Fang , Wu Dan , Zhe Xiangyi , Xin Huizhen , Li Hongtao , Li Dongmei , Jin Fuyuan , Shao Renfu , Pan Zemin 
Published: July 2020

Abstract
Background This study aimed to determine whether ZNF582 gene methylation and tissue protein expression can be used as a tool with high sensitivity and specificity for cervical cancer screening. We analyzed the correlation between promoter methylation of the zinc finger protein 582 (ZNF582) gene and cervical cancer and high risk HPV16/18 infection. Methods Tissue samples of normal cervical or chronic cervicitis (n=51), CIN (cervical intraepithelial neoplasia) (n=35), and cervical carcinoma (n=68) were tested for HPV16/18 infection by polymerase chain reaction (PCR). We also detected the methylation status of the ZNF582 gene promoter in the same tissues by methylation specific PCR (MSP), then analyzed the correlation between ZNF582 promoter methylation and HPV16/18 infection. Immunohistochemistry was used to analyze ZNF582 gene expression in 152 cervical tissues. We detected ZNF582 mRNA expression in cervical tissues (including cancer and non-cancer) by real-time fluorescence quantitative PCR (qRT-PCR).Results Among 93 high grade cervical lesions (CINII and above) and cervical cancer samples, 57 cases were positive for HPV16/18 infection and 36 cases were negative. ZNF582 gene methylation occurred in 9 out of 51 cases in normal cervical tissues (17.6%), 16 of 35 cases in CIN tissues (45.7%), and 50 of 68 cases in cervical cancer (73.5%). The differences in methylation rate of the three groups were statistically significant (P<0.05). The ZNF582 methylation rate in the positive HPV16/18 infection group was 73.7%, while the negative group was 63.9%. Compared with normal tissues, ZNF582 protein was highly expressed in cervical cancer tissues, but mRNA expression was low.Conclusion While ZNF582 protein is highly expressed in cervical cancer tissues, it was not sufficient for use as a standard for cervical cancer staging. On the other hand, ZNF582 promoter methylation had high specificity and sensitivity in detecting CINII and highly diseased cervical lesions and could be used as a diagnostic marker for cervical cancer of women.
Prognostic significance of lymph node dissection on stage-specific survival for patients with primary small intestine tumor treated with enterectomy: A population-based study, 2004-2015
Authors: Chen Yifei , He Fei , Guo Dan , Li Yarui , Wang Ruhua , Zhang Xu , He Shuixiang 
Published: July 2020

Abstract
Background: The positive rate of lymph node detection(LND) can be used as a predictor of prognosis for patients undergoing radical resection of small bowel tumors; thorough local LND may be crucial for the accurate staging and management of the disease.The purpose of our study was to determine the effect of the LND in specific stages. Methods: This study included 5413 patients with primary small intestine tumors after enterectomy within SEER database from 2004-2015. A multivariable COX model and Kaplan-Meier plots survival curves were used to analyze survival.Results: Of the 5413 patients, 4675(86.4%) underwent lymphadenectomy, and 3896(72.0%) were moved 4 or more than 4 lymph nodes. LND was performed in 67.8%, 83.3%, 87.9%, 89.3% in pT1/2/3/4 disease. In multivariable Cox regression analyses, LND was associated with OS and CSS, and the extended LND are better than limited LND (all P<0.05 except pT2). Kaplan-Meier plots survival curves showed that LND can benefit patients.Conclusions: The removal of LND with 4 or more lymph nodes in pT1/3/4 patients has relatively obvious benefits for survival. The effect of LND with more lymph nodes is significantly better than limited LND. For pT1, pT3 and pT4, LND can be considered.
Persistently Gadolinium Enhancing Lesion is Predictor of Poor Prognosis in NMOSD Attack: A Clinical Trial
Authors: Xu Yun , Li Xindi , Xu Wangshu , Wang Xinli , Duan Yunyun , Liu Yaou , Zhang Xinghu , Tian De-Cai 
Published: July 2020

Abstract
Background: Gadolinium (Gd)-contrast MRI, reliable detecting blood-brain barrier (BBB) breakdown, is widely used in neuromyelitis optica spectrum disorder (NMOSD) attack. To investigate the predictive value of persistently Gadolinium-enhanced lesions to long-term outcome after attack.Methods: Data for this analysis come from an ongoing prospective cohort study to assess the clinical and imaging patterns of neuroinflammation diseases in China (CLUE). NMOSD patients with acute attack were enrolled from January 2019 to March 2020. All patients underwent Gadolinium-contrast Magnetic Resonance Imaging (MRI) at baseline and 1 month, and disability was assessed using the Expanded Disability Status Scale (EDSS) at baseline and month 1 and 6. Primary outcome was EDSS score improvement from baseline to month 6. Multiple logistic regression identified predictors for poor recovery of NMOSD attack.Results: 41 participants were analyzed, of which 21 patients with persistently Gd-enhancing lesions. Patients in non-continuous enhancement (nCE) group showed statistically significant shift in the 6-month EDSS distributions compared with continuous enhancement (CE) group (p = 0.002). Poor recovery rate of CE group was higher than that of nCE group at 6 months (p = 0.033). In patients with AQP4-IgG positive, first attack, transverse myelitis or high-dose steroid treatment, the improvement of EDSS scores in CE group was still less compared to nCE group (p < 0.05). The presence of persistently Gd-enhancing lesion appears to be associated with poor recovery after acute attack (OR = 5.473, 95% CI 1.417-21.115, p = 0.014).Conclusion: NMOSD patients with persistently gadolinium-enhancing lesions have worse outcome at 6 months. Persistently Gd-enhancing lesion is a poor prognosis predictor after NMOSD attack.Trial registration: This clinical trial named Clinical and Imaging Patterns of Neuroinflammation Diseases in China (CLUE), which has registered with ClinicalTrials.gov (NCT04106830). The date of registration was September 27, 2019 (Retrospectively registered). The URL is https://clinicaltrials.gov/ct2/show/NCT04106830?term=NCT04106830&cntry=CN&draw=2&rank=1.
Two Novel Likely Pathogenic Variants of HARS2 Identified in a Chinese Family With Perrault Syndrome
Authors: yu jing , jiang Wei , cao Li , Na xiaoxue , Yang jiyun 
Published: July 2020

Abstract
HARS2 is one of the genetic causes of Perrault syndrome, characterized by sensorineural hearing loss (SNHL) and ovarian dysfunction. Here, we identified two novel putative pathogenic variants of HARS2 in a Perrault syndrome pedigree including two affected male siblings, c.349G>A (p.Asp117Asn) and c.908T>C (p.Leu303Pro), through targeted next-generation sequencing methods. The two affected siblings (13 and 11 years old) presented with early-onset, rapidly progressive SNHL. The affected siblings did not have any inner ear malformations or delays in gross motor development. Combined with preexisting clinical reports, these findings further expanded the existing spectrum of HARS2 variants and Perrault syndrome phenotypes, which will assist in molecular diagnosis and genetic counselling of patients with Perrault syndrome.
How do Iranian High School Students Explain Social Undesirability of Hookah Smoking? A Qualitative Study
Authors: Keshavarzian Khadijeh , Mohammadpourasl Asghar , Allahverdipour Hamid , Nadrian Haidar 
Published: July 2020

Abstract
Background: Despite the speculations in literature regarding the importance of “social undesirability of hookah smoking”, to our knowledge, no study has yet clearly attempted to define this phenomenon and to make sense of it in terms of the meanings adolescents bring to the concept. The present study was aimed to explore the perceptions of high school students on Social Undesirability of Hookah Smoking (SUoHS).Method: In this qualitative study, 31 students (16 boys and 15 girls) at the range of 15 to 17 years of age in Tabriz, Iran, were invited to participate in semi-structured individual interviews (18 participants) and focus group discussions (FGDs) (13 participants). The data were managed applying MAXQDA-10, and analyzed using interpretative thematic analysis. Results: The SUoHS was viewed at the “highly negative attitude towards hookah smoking and hookah smokers”. The students also explained the SUoHS in “the disvaluing to hookah smoking and smokers in the society”, “the negative attitude towards hookah smokers and their families”, and “the consequences of hookah smoking”. Conclusion: The SUoHS originates from the social context of society, and is rooted from the social values and norms within communities. These social values may affect the attitude and subjective norms of adolescents toward the behavior. Adolescents’ conceptions on SUoHS should be considered while designing hookah smoking prevention/cessation programs with the hope to bridge the gap between their recognition of SUoHS and subsequent behavior change.
Temporomandibular Joint Hypermobility - Diagnosis Based on Auscultation and Signals Analysis
Authors: Lemejda Justyna Agnieszka , Kajor Marcin , Grochala Dominik , Iwaniec Marek , Iwaniec Joanna , Loster Jolanta E. 
Published: July 2020

Abstract
BackgroundThe temporomandibular joint is a well-known anatomical structure a vast majority of dentists doesn’t know how to treat patients with temporomandibular disorders. What is more, even physiological sounds accompanying joint movement can deceitfully indicate pathological features. An example of TMD is temporomandibular joint hypermobility, a disorder which still requires comprehensive study and analysis.MethodsForty seven volunteers were diagnosed with the RDC/TMD questionnaire and auscultated with electronic stethoscope Littmann 3200 on both sides simultaneously. Recorded TMJ sounds were sent to the computer via Bluetooth for further numerical analysis.ResultsTwo patients with TMJH were diagnosed. In the first case the RDC/TMD based diagnosis were discovered as Ia (myofascial pain) and IIIa (arthralgia). The second person was diagnosed with no disorder according to RDC/TMD questionnaire. However the RDC/TMD examination does not support recognition of TMJH. Therefore in this paper it was proved that such recognition can be performed based on the digital time-frequency analysis of the TMJ sound.ConclusionsTMJH is rare case, at ours research 4% patients. The recognition of dysfunction can be based on the characteristic time-frequency features presented in the spectrogram representation of TMJ sound.Trial registration﻿The Jagiellonian University Bioethics Committee gave consent for examination, number 1072.6120.71.2019 from the 24th of April 2019 year. All participants were informed about the objectives of the study. Each of them expressed their written consent to participate in the examination and signed the consent form before the study. During the procedures, the rules of Good Clinical Practice were applied and the Helsinki Declaration was followed.
Antibiotic Appropriateness and Adherence to Local Guidelines in Perioperative Prophylaxis: Results from an Antimicrobial Stewardship Intervention
Authors: Segala Francesco Vladimiro , Murri Rita , Taddei Eleonora , Giovannenze Francesca , Vecchio Pierluigi Del , Birocchi Emanuela , Taccari Francesco , Cauda Roberto , Fantoni Massimo 
Published: July 2020

Abstract
Objectives: Surgical antibiotic prophylaxis (SAP) represents a major indication of antibiotic consumption worldwide. The present study aims to report the results of an enabling, long-term AMS intervention conducted between 2013 and 2019 on an Italian University Hospital performing more than 40.000 surgical interventions per year.Methods: SAP inappropriateness was defined according to the ASHP guidelines and divided in four main categories: indication, selection and dosing, duration, timing. Between 2013 and 2019, we conducted a continuative AMS intervention over 14 surgical departments that included enablement, review of selected clinical records and feedback. Results: We collected a total of 789 SAP prescribed to 735 patients (mean age 56.7 ± 17.8y). Overall, guideline adherence improved from 36.6% (n = 149) at baseline to 57.9% (n = 221) post-intervention (P<0.0001). A significant improvement (P<0.001) was also detected for each category: indication (from 58.5% to 93.2%), selection and dosing (from 58.5% to 80.6%), timing (from 92.4 to 97.6%), duration (from 71% to 80.1%). Conclusions: Though results cannot be generalized to all hospital populations, enabling AMS interventions may be effective in establishing a sustained improvement in SAP appropriateness rates. Once identified the main causes of SAP inappropriateness, tailored AMS interventions for each department may be beneficial. Further studies are needed to evaluate specific outcomes as incidence of surgical site infections and antimicrobial resistance.
Reducing dietary cation–anion difference seldom affects rumen fermentation, cellulolytic bacteria populations, and microbiota in goats
Authors: Yang Kang , Tian Xingzhou , Jian Shiyan , Sun Lun , Han E , Wang Fei , Guo Ziyi , Ma Zhengfa , He Tengbing , Wu Wenxuan 
Published: July 2020

Abstract
Background: Dietary cation–anion difference (DCAD) has been receiving increased attention in recent years; however, information on the rumen fermentation, cellulolytic bacteria populations, and microbiota of goats fed a low-DCAD diet is less. This study aimed to evaluate the feasibility of feeding a low-DCAD diet for goats with emphasis on rumen fermentation parameters, cellulolytic bacteria populations and microbiota. Growth performance, urine pH, and plasma metabolites were also analyzed as well. Materials and method: Eighteen goats were randomly allocated to 3 treatments with six replicates of each treatment and 1 goat per replicate. Animals were fed diets with varying DCAD levels at +338 (High DCAD; HD), +152 (Control; CON), and −181 (Low DCAD; LD). This study includes 15-d experimental period and 30-d adaption period. Results: The DCAD level did not affect the rumen fermentation parameters including pH, buffering capability, acetic acid, propionic acid, butyric acid, total volatile fatty acids, and ratio of acetic acid/propionic acid (P > 0.05). The 4 main ruminal cellulolytic bacteria populations including Fibrobacter succinogenes, Ruminococcus flavefaciens, Butyrivibrio fibrisolvens and Ruminococcus albus did not differ from DCAD treatments (P > 0.05). The DCAD levels did not affect bacterial richness and diversity indicated by the indices Chao, Ace and Simpson and Shannon, respectively (P > 0.05). Both weighted UniFrac and unweighted UniFrac showed no difference in the composition of rumen microbiota for CON, HD and LD (P > 0.05). At the phylum level, Bacteroidetes was the predominant phylum followed by Firmicutes, Synergistetes, Proteobacteria, Spirochaetae, and Tenericutes, and they showed no difference (P > 0.05) in relative abundances except for Firmicutes, which was higher in HD and LD compared to CON (P < 0.05). At the genus level, relative abundance of 11 genera were not affected by DCAD treatments (P > 0.05). Level of DCAD had no effect (P > 0.05) on growth performance including dry matter intake, average net gain, average daily gain, and feed conversion ratio; and nutrients digestibility of crude protein, neutral detergent fiber, acid detergent fiber, and organic matter (P > 0.05). Urine pH in LD was lower than HD and CON (P < 0.05). LD resulted in higher plasma calcium than HD and CON (P < 0.05) but not for other plasma metabolites (P > 0.05). Conclusion: We conclude that, with regard to the great importance of rumen fermentation, these results suggest that reducing DCAD is unharmful for rumen status and provide the feasibility of feeding a low-DCAD to goats.
A Conceptual Framework for Mental Model-based Repeated Multifaceted Intervention Design to Improve Preventive Health Behaviors and Outcomes
Authors: Ahamad Mazbahul G , Tanin Fahian 
Published: July 2020

Abstract
Background: Improving the effectiveness of health interventions is a major challenge in public health research and program development. A large body of literature found low or no impact of health education and promotional interventions, especially in low- and middle-income countries.Objectives: To develop a conceptual framework in support of intervention designs of preventive health behavior improvement programs and outcomes.Methods: An overview of systematic and scoping reviews along with empirical literature was conducted to assess the limitations of preventive health education and promotion interventions using water, sanitation, and hygiene (WASH) programs and outcomes as examples.Results: We analyzed the limitations of the less effective or ineffective field interventions cited in peer-reviewed articles. We found three major limitations regarding the mental model’s balance of treatment and comparison groups, treatment groups’ willingness to adopt suggested behaviors, and the type, length, frequency, intensity, and sequence of treatments. To minimize the influence of these concerns, we proposed a mental model-based repeated multifaceted (MRM) intervention design framework.Conclusions: Our proposed framework aims to provide an intervention design for improving health education and promotional programs. Further studies using this framework are essential to examine our hypotheses and advance the understanding of how MRM design can improve preventive health behaviors and outcomes.
Effect of Echinacea purpura’s extract on the expression of VEGF-A gene and antiproliferative, induction of apoptosis, inhibition of cell migration, and colony formation on gastric cancer cell line AGS
Authors: Moradian Fatemeh , Sadeghi Azar , Sabouni Farzaneh , Sanjarian Forough 
Published: July 2020

Abstract
The authors have requested that this preprint be removed from Research Square.
PGK1, a promising biomarker and therapeutic target for patients with acute myeloid leukemia who are susceptible to disease relapse
Authors: Gao Junyan , Chu Xinran , He Shan , Gao Li , Hou Hui , Fang Fang , Pan Jian , Hu Shaoyan 
Published: July 2020

Abstract
Background: Glycolysis, a multi-step enzymatic reaction, is considered to be the root of cancer development and progression. The aim of this study is to figure out the glycolytic enzyme, phosphoglycerate kinase 1 (PGK1) whether participate in the progression of acute myeloid leukemia (AML) and its possible mechanisms. Methods: Four datasets (GSE106096, GSE75086, GSE107968 and GSE106748) containing 30 leukemic blast cell samples of AML at diagnosis, 17 leukemic blast cell samples of AML relapse and 3 bone marrow CD34+ cell samples of healthy donors were downloaded from Gene Expression Omnibus (GEO) database and PGK1 was screened out as a potential survival biomarker in AML. Then we did a series of clinical sample verifications and gene set enrichment analysis (GSEA) focusing on PGK1. We further knocked down expression of PGK1 in myelogenous leukemia cell lines and explored its potential effects. Results: PGK1 expression was up-regulated among AML at diagnosis versus healthy control, AML relapse versus AML at diagnosis and AML relapse versus healthy control datasets. Through a serial of bioinformatic analyses (differentially expressed genes [DEGs] selection, function and pathway enrichments and protein-protein interaction [PPI] network establishment), PGK1 was identified as the most meaningful gene in AML progression. Furthermore, the generally high expression of PGK1 was confirmed in AML samples comparing with healthy controls in our single center and the high-expression PGK1 was associated with a comparatively low complete remission (CR) rate, a significantly high 5-year cumulative incidence of relapse (CIR), a poor 5-year event-free survival (EFS) rate, and a poor 5-year overall survival (OS) rate. The GSEA revealed that high-expression PGK1 in AML was associated with many pathways including cytosolic DNA sensing, pentose phosphate, base excision repair and DNA replication. In vitro, the transfected U937 and K562 cells with PGK1 knock-down showed decreased cell viability and increased apoptotic rate. PGK1 inhibition could greatly decrease the half maximal inhibitory concentrations (IC50) of cytarabine (Ara-C) and daunorubicin (DNR) in U937 and K562 cell lines.Conclusions: High-expression PGK1 was associated with poor prognosis in AML. PGK1 may serve to predict the AML progression and provide a novel therapeutic target for AML.
Conceptualizations of Clinical Decision-Making: A Scoping Review in Geriatric Emergency Medicine
Authors: Gamborg Maria Louise , Mehlsen Mimi , Paltved Charlotte , Tramm Gitte , Musaeus Peter 
Published: July 2020

Abstract
Background: Clinical decision-making (CDM) is an important competency for young doctors, especially under complex and uncertain conditions, which is present in geriatric emergency medicine (GEM). Research in this field is however characterized by an unclear conceptualization of CDM. To evolve and evaluate evidence-based knowledge of CDM, it is thus important to identify different definitions and their operationalisations in studies on GEM.Objective: A scoping review of empirical articles was designed to provide an overview of the documented evidence of findings and conceptualizations of CDM in GEM.Methods: A detailed search for empirical studies focusing on CDM in a GEM setting was conducted in PubMed, ProQuest, Scopus, EMBASE and Web of Science. In total, 52 publications were included in the analysis, utilizing a data extraction sheet, following the PRISMA guidelines. Reported outcomes were summarized.Results: Four themes of operationalization of CDM emerged; CDM as dispositional decisions, CDM as cognition, CDM as a model, and CDM as clinical judgement. Study results and conclusions differed according to how CDM was conceptualized. It was evident how especially frailty- heuristics lead to biases in treatment of geriatric patients, and that the complexity of this patient group was seen as a challenge for CDM.Conclusions: This scoping review summarizes how different studies in GEM use the term CDM. It provides a snapshot of findings in GEM. Potentially, findings from CDM research can guide implementation of adequate CDM strategies in clinical practice but this requires application of more stringent definitions of CDM in future research.