bluuebunny/crossref_metadata_2025_split huggingface dataset:
CCL1 blockade alleviates human mesenchymal stem cell (hMSC)-induced pulmonary fibrosis in a murine sclerodermatous graft-versus-host disease (Scl-GVHD) model
Authors: Lim Ji-Young , Ryu Da-Bin , Kim Tae Woo , Lee Sung-Eun , Park Gyeongsin , Yoon Hyoung Kyu , Min Chang-Ki 
Published: May 2020

Abstract
Background Human chronic graft-versus-host disease (CGVHD) shares clinical characteristics with a murine sclerodermatous GVHD (Scl-GVHD, B10.D2 → BALB/c) model that is characterized by skin and lung fibrosis. In this study, bone marrow- or adipose tissue-derived human mesenchymal stem cells (hMSCs) were injected into the Scl-GVHD mice to address their therapeutic effect on CGVHD. Methods Lethally irradiated BALB/c mice were transplanted with B10.D2 T cell depleted bone marrow with or without spleen cells to generate Scl GVHD. hMSCs were intravenously treated on days 3, 5 and 7 post-transplantation. And the control antibody or CCL1 blocking antibody was subcutaneously injected into the same schedule as hMSCs. At 14 days after transplantation, the recipient mice sacrificed and their skin and lung analyzed. Results After early injection of hMSCs after transplantation, the clinical and pathological severity of Scl-GVHD in the skin was significantly attenuated, whereas the pathological score was exacerbated in the lungs. hMSCs migrated into the lungs but not into the skin. CD11b monocyte/macrophages and CD4 T cells were markedly decreased in skin tissues, whereas there was an early recruitment of CD11b cells, and subsequently increased infiltration of CD4 T cells, in the lungs. Importantly, hMSCs persistently up-regulated the expression of CCL1 in the lungs but not in the skin. Concurrent treatment of hMSCs with a CCL1-blocking antibody alleviated the severity of the lung histopathology score and fibrosis with preservation of the cutaneous protective effect against CGVHD. Infiltration of CD3 T cells and CD68 macrophages and up-regulation of chemokines were also decreased in lung tissues, along with recruitment of eosinophils and tissue IgE expression. In the skin, chemokine expression was further reduced after CCL1 blockade. Conclusions These data demonstrate that despite a protective effect against Scl-GVHD in the skin, administration of hMSCs exacerbated lung fibrosis associated with eosinophilia and airway inflammation through persistent CCL1 up-regulation. CCL1 blockade offers a potential treatment of pulmonary complications induced after treatment with hMSCs.
People’s perception of quarantine during the COVID-19 outbreak in Shantou, China
Authors: Deng Xiulian , Chen Shaoxing , Zhou Yilian , Zhang Lishao , Chen Xiaojun 
Published: May 2020

Abstract
Background

Unprecedented public health measures have been implemented to combat the pandemic outbreak of the 2019 coronavirus disease (COVID-19) in China. The purpose of this research is to identify people’s perception of quarantine during the COVID-19
Methods

Semi-structured interviews with 30 participants were conducted, including 15 people who had been quarantined and 15 participants who had not been quarantined during the COVID-19 outbreak in Shantou, Southern China. Interview data were collected from February to March 2020. All interviews were audio-recorded and transcribed verbatim. The transcribed data were coded and analyzed to identify key themes.
Results

Four key themes concerning public health measures emerged: 1) A city-wide lockdown effectively aroused the public’s awareness of the seriousness of COVID-19, which was helpful for controlling the pandemic; however, it greatly impacted the economy and individuals’ way of life. 2) Hotel quarantine was an effective measure for medical observations. 3) Fear and lack of knowledge of quarantine were the main factors that affected quarantine compliance. 4) Less attention was paid to people’s mental health during the pandemic outbreak.
Conclusions

A lockdown and centralized hotel quarantines were effective ways to control the outbreak. Mandatory measures were highly supported. The media should educate the public about COVID-19. Psychological counseling is necessary for people who were quarantined long-term. The findings have implications for other countries and for emerging infectious diseases in the future.
Uptake of Misoprostol among women seeking abortion in Accra, Ghana
Authors: Darteh Eugene Kofuor Maafo 
Published: May 2020

Abstract
Background Unsafe abortions remain one of the key contributors to maternal mortality. The World Health Organisation (WHO) has recommended misoprostol as a safe abortifacient which has become a popular over the counter self-administered abortifacient in Ghana. This study sought to examine the prevalence of misoprostol uptake and model the factors associated with its uptake among 698 women seeking abortion services in the Accra Metropolis of Ghana. Methods Univariate and multivariate analysis were done. The multivariate analysis was done using binary logistic regression to model the factors associated with misoprostol uptake and results are presented as adjusted odds ratios (AORs) with their 95% confidence intervals (CIs). P < 0.05 was considered statistically significant. Results The results showed that the prevalence of misoprostol uptake was 20.8%. The regression analysis showed that women who were cohabiting had higher odds [AOR=1.91, CI=1.16-3.16] of using misoprostol compared to women who were single. In relation to miscarriage and use of misoprostol, it was found that women who experienced one [AOR=0.27, CI=0.15-0.49] and 2 or more miscarriages [AOR=0.25, CI=0.07-0.85] had lower odds of using misoprostol compared to those who have never experienced any miscarriage Conclusion The prevalence of misoprostol uptake among women seeking abortion services in Accra Metropolis is relatively high. Marital status and previous history of miscarriage are associated with misoprostol uptake. Although proven effective by various studies and recommended by the WHO, it is imperative to consider these factors in the education of women on the use of misoprostol as well as designing programmes to target women who are inclined to induce abortion in Ghana.
A Study of the Characteristics of the White Matter in Schizophrenic Patients with Defective or Non-defective Symptoms by Diffuse Tensor Imaging
Authors: Shi Wen-jie , Sun Tai-peng , Zhuang Li-rong , Feng Hua , Yan Peng , Wang Cong-jie , Wang Zheng , Huang Cheng-bing 
Published: May 2020

Abstract
BackgroundDeficit schizophrenia (DS) is a set of highly homogenous schizophrenia subtypes characterized by primary and persistent negative symptoms. Previous research studies have found that the negative symptoms of schizophrenia are closely related to the impairment of brain structure and function.This study seeks to explore the characteristics of white matter in schizophrenic patients with defective or non-defective symptoms by diffuse tensor imaging (DTI).MethodsAccording to the defective schizophrenia diagnostic criteria and ICD-10 diagnostic criteria, 30 patients with DS and 30 patients with non-defective schizophrenia (NDS) were enrolled into the research study. DTI imaging data of the white matter were collected by 1.5T magnetic resonance imaging scanner. Then a tract-based spatial statistics (TBSS) method was used to compare the fractional anisotropy (FA) values of the white matter fiber between the two groups.ResultsThe TBSS analysis results showed that the FA values in the right side of the knee of the corpus callosum (MNI:14,36,-7), right anterior radio-coronal region (MIN:11,34,3) and the right hippocampal region (MIN:30,34,16) in the DS patients were significantly lower compared with those of the NDS patients (all p<0.05). The FA values in the right side of the knee of the corpus callosum was significantly correlated with the time from onset to treatment (r=−0.350, p<0.001), PANSS-negative symptom score (r=−0.157, p=0.007). The FA values in the right anterior radio-crown region was positively correlated with PANSS-negative symptom score (r=0.306, p=0.048). The right hippocampus was negatively correlated with years of education (r=−0.614, p=0.020), duration of antipsychotics using(r=−0.140, p= 0.022), and PANSS-negative symptom score (r=−0.637, p=0.040).ConclusionsIn schizophrenic patients with defective symptoms, the structural integrity of white matter fibers was more seriously damaged in the three brain regions including the right knee of the corpus callosum, the right anterior region of the right radiative crown, and the right hippocampus. These white matter lesions are closely related to patient characteristics such as years of education, duration from onset to treatment, duration of anti-psychotic, and severity of negative symptoms.
Reginal Lymph Node Involvement is Associated With Poorer Survivorship in Patients With Malignant Bone Tumor
Authors: Xie Xianbiao , Zou Yutong , Wen Lili , Lv Dongming , Zeng Ziliang , Jin Qinglin , Bian Yiying , Yao Hao , Shen Jingnan 
Published: May 2020

Abstract
Background

Regional lymph node involvement is rare in patients with malignant bone tumors. We aimed to analyze the prevalence and prognostic implications in the lymph-involved patients with malignant bone tumors.

Materials and Methods

From 1988 to 2016, 9582 patients with primary malignant bone tumors in the SEER database were enrolled. Overall survival (OS) was computed using the Kaplan-Meier method. A multivariate analysis was performed using the Cox regression.

Results

346 (3.63%) patients exhibited regional lymph node involvement. Lymph-involved patients had larger tumor size and more metastasis than patients without. Ewing sarcoma most frequently developed lymph node involvement. Lymph-involved patients (0.31 95% CI (0.26–0.37)) had lower 5-year OS rates than patients without lymph node involvement (0.66 95% CI (0.65–0.68)) (p<0.001).

Conclusions

Lymph node involvement is rare in patients with malignant bone tumors. They had a large tumor size, more distant metastasis and poor survival.
Reporting on the Opioid Crisis (2000-2018) – the Globe and Mail, Canada’s English Language Paper of Record
Authors: Quan Amanda , Wilson Lindsay A. , Wilson Kumanan 
Published: May 2020

Abstract
Objectives: We aim to describe the general characteristics of how Canada’s newspaper of record – The Globe and Mail, reports on opioid-related news, the opioid crisis and its victims,and explore how Canadians’ perceptions of the opioid crisis could have developed over time.Methods: We searched The Globe and Mailbetween 2000 and 2018. We identified all articles related to the keyword “opioids”. Independently and in duplicate, reviewers extracted qualitative data from articles. The Social Representation Theory was used as a framework for understanding the how the opioid crisis is portrayed in Canada. Results:Our search yielded 554 relevant opioid articles.The number of articles peaked in 2009, 2012, and in 2016, coinciding with major developments in the epidemic. The language used in this discourse has evolved over the years and has slowly shifted towards less stigmatizing language. Content analysis of the articles revealed common social representations attributing blame to pharmaceutical companies, physicians, and foreign countries.It is easy to blame these collectives as this contributes to social representations thatare anchored in thepublic’s predisposed notions.Conclusions:Canadian coverage of the opioid crisis is focused on basic social representations and blame patterns towards a few collectives, a shift towards root causes of the opioid epidemic could positively influence the general public’s perception of the opioid crisis and help reap deeper understanding of the issue. Journalists face several obstacles to achieve greater focus and framing of the opioid crisis, a closer working relationshipbetween the media and the research community is needed.
Lack of the Flavin-Containing Monooxygenase FmoA Partially Impairs the Symbiotic Interaction of Mesorhizobium Huakuii with Astragalus Sinicus
Authors: Wu Hetao , Zou Qian , Luo Sha , He Donglan , Li Xiaohua , Yan Chunlan , Cheng Guojun 
Published: May 2020

Abstract
Background: Flavin-containing monooxygenases (FMOs) catalyze the NADPH-dependent N- or S- oxygenation of numerous foreign chemicals, and thus may mediate interactions between microorganisms and their chemical environment. The aim of this study was to investigate the role of FMO in symbiotic nitrogen fixation of Mesorhizobium huakuii and its host plant Astragalus sinicus.Results: A mutation in M. huakuii fmoA gene was generated by homologous recombination. The fmoA mutant grew more slowly than its parental strain, and displayed decreased antioxidative capacity under higher concentration of H2O2 and cumene hydroperoxide (CUOOH), indicating that FmoA plays an important role in response to the peroxides. The fmoA mutant strain displayed no difference of peroxidase activity and glutathione reductase activity, but significantly lower level of glutathione and hydrogen peroxide content than the wild type. Real-time quantitative PCR showed that the fmoA gene expression is significantly up-regulated in three different stages of nodule development. The fmoA mutant was severely impaired in its rhizosphere colonization, and its symbiotic properties in Astragalus sinicus were drastically affected. Transcriptomes in root-nodule bacteroids were analyzed and compared. A total of 1233 genes were differentially expressed, of which 560 were up-regulated and 673 were down-regulated in HKfmoA bacteroids compared with that in 7653R bacteroids. The transcriptomic data allowed us to determine additional target genes, whose differential expression was able to explain the observed the changes of symbiotic phenotype in the mutant-infected nodules.Conclusions: The fmoA gene is essential for antioxidant capacity and symbiotic nitrogen fixation. Furthermore, RNA-Seq based global transcriptomic analysis provided a comprehensive view of M. huakuii fmoA gene involved in nodule senescence and symbiotic nitrogen fixation.
The Failure Risk of Surgical Therapy for Corneal Perforations Using Cryopreserved Donor Corneas
Authors: Mizuno Miki Hata , Uchino Yuichi , Shimmura Shigeto , Tsubota Kazuo 
Published: May 2020

Abstract
Background To evaluate the long-term results of therapeutic keratoplasty for a consecutive case series of corneal perforation.Methods The cases comprised 41 eyes (41 patients) that underwent therapeutic keratoplasty using cryopreserved donor grafts at the Keio University Hospital between January 2012 and December 2016. The eyes were evaluated regarding the cause of corneal perforation, surgical procedure, size of the perforation, presence of anterior chamber collapse, visual prognosis, and complications.Results The major causative diseases included herpetic keratitis (n = 11), bacterial and fungal corneal ulcer (n = 4), Mooren’s ulcer (n = 5), severe dry eye (n = 4), and neuroparalytic keratitis (n = 4). Penetrating keratoplasty was performed in 28 eyes (68.2%), lamellar keratoplasty in 8 eyes (19.5%), and keratoepithelioplasty in 5 eyes (12.2%).
FT3 to FT4 Ratio is Associated with Non-Alcoholic Steatohepatitis and Significant Fibrosis in Euthyroid Subjects&nbsp;with NAFLD
Authors: Xue Haibo , Guan Yaqi , Zheng Juzeng , Xia Ziqiang , Li Ye , Wu Jinming 
Published: May 2020

Abstract
Background & Aims: Studies on the relationship between thyroid function and non-alcoholic fatty liver disease (NAFLD) among euthyroid subjects had shown inconsistent results. Objective of the present study was to exploring the independent relationship between thyroid function parameters and nonalcoholic steatohepatitis (NASH), significant fibrosis (SF) respectively after adjusting other well-identified risk factors.Method: This study enrolled 307 patients with biopsy-proven NAFLD. Thyroid dysfunction defined as serum thyroid-stimulating hormone > 4.5 mIU/l or < 0.5 mIU/l and/ or free thyroxine > 14.41 pmol/l or < 7.86 pmol/l.Results: Stepwise regression analysis showed that the fT3/fT4 ratio, an optimal thyroid function parameter, was associated with NASH and SF in euthyroid subjects with NAFLD. After multivariable analysis, the fT3/fT4 ratio (per 0.1 change) showed significant correlation with NASH (OR 2.03(1.33, 3.11), P = 0.001) and SF (OR 2.11(1.28, 3.46), P = 0.003). When ratio was stratified by quartiles (Q1-Q4) as a categorical variable, the results still significant (Q4 versus Q1 (OR for NASH 4.29(1.68, 10.91), P = 0.002; for SF 4.63(1.49, 14.43), P = 0.008, all P for linear trend < 0.05). The prevalence of NASH and SF rose significantly with increasing in quartiles. Furthermore, ratio was positively correlated with the grade of steatosis, lobular inflammation, hepatocellular ballooning and liver fibrosis stage (all P < 0.05). Subgroup analysis showed that hypertension was a possible effect modification.Conclusion: The findings of the present study confirmed an association between fT3/fT4 ratio and NAFLD in euthyroid subjects in a dose-dependent manner, particularly in non-hypertension adults.There is no trial registration number.
The relationship between counselling methods after health check-ups and lipid profile improvement: a retrospective cohort study in Korea
Authors: Kim Na yeon , Kim Jung-Ha , Cho Soo Hyun , Kim Seon Ah 
Published: May 2020

Abstract
Background

Despite growing numbers of private health check-ups, it is not known whether post-check-up counselling and education can improve chronic disease management. It has previously been shown that, in general, these factors are crucial to chronic disease management. Therefore, this study aimed to determine which counselling methods should be employed, following private check-ups, for optimal chronic disease management.
Methods

Participants were 7,039 adults over the age of 20, who received at least three check-ups from September 2013 to August 2019. All participants received the same form of counselling, three or more times consecutively. Three forms of counselling were examined: mail, telephone, and face-to-face. Chi-square tests, one-way analyses of variance, and Scheffé post-hoc analyses were performed to determine the relationship between various demographic characteristics and counselling methods received. We performed covariance analyses after adjusting for age, sex and number of examinations to determine the correlations between the counselling methods and changes in health indicators. When necessary, Bonferroni pairwise comparisons were performed.
Results

The face-to-face counselling group was the oldest and had the poorest cardiometabolic parameters and glucose metabolic indices. However, face-to-face counselling was associated with the greatest improvement in levels of total cholesterol (P < 0.001) and low-density lipoprotein cholesterol (P < 0.001).
Conclusion

Face-to-face counselling with doctors seems to be more effective at improving lipid profiles than phone or mail counselling. Further research is required to identify the effects of face-to-face counselling on long-term outcomes such as cardiovascular disease mortality. (IRB number: 1909-006-16282).
MiR-223 Derived from Mesenchymal Stem Cell Exosomes Alleviates Acute Graft-Versus-Host Disease
Authors: Liu Weijiang , Zhou Na , Wang Peng , Liu Yuanlin , Zhang Wei , Li Xue , Wang Yang , Zheng Rongxiu , Zhang Yi 
Published: May 2020

Abstract
Background Mesenchymal stem cells (MSCs) have been utilized in treating acute graft-versus-host disease (aGvHD) as they show strong immunosuppressive capacity, but the mechanisms are not well defined.Methods In this study, we demonstrated that microRNA-223 (miR-223) derived from exosomes secreted by human umbilical cord mesenchymal stem cells (huc-MSCs) and murine compact bone mesenchymal stem cells (mb-MSCs) could inhibit aGvHD progression by reducing the migration and homing of donor T cells in aGvHD mice.Results MiR-223 was one of the conserved microRNAs highly expressed in huc-MSCs exosomes and mMSCs exosomes, which was identified by high-throughput sequencing. MiR-223 derived from MSC exosomes showed enhanced immunosuppressive capacity, as it could inhibit expression of the target gene ICAM-1 and restrain adhesion and migration of T cells in vitro. Moreover, miR-223Agomir was effective in reducing the inflammatory reaction, and declining the donor T cells infiltration into the spleen, liver and intestine in aGvHD mice. Subsequently, it could alleviate aGvHD symptoms. Taken together, the MSC exosome derived miR-223 could attenuate aGvHD in mice through regulating ICAM-1 expression.Conclusions Our results unveil a new role for MSC exosomes derived miR-223 in the treatment of aGvHD.
Mapping Research in Joint Preserving Procedures of Osteonecrosis of the Femoral Head: A Bibliometric Analysis
Authors: Zhang Qing Xi , Zhao Yu , LI Hui , Xing Dan , Lin Jianhao 
Published: May 2020

Abstract
Background: There have been increased interests in the joint preserving procedures of osteonecrosis of the femoral head (ONFH). This study aims to present a bibliometric analysis of the publications of this field during this period (1999–2019).Method: Publications related to joint preserving procedures of ONFH were searched from the Science Citation Index-Expanded Web of Science. The data were analyzed by using bibliometric methodology. Additionally, the work made a graphical mapping by using the VOS viewer software. This was used for bibliographic coupling, co-authorship, co-citation, co-occurrence analysis and to investigate the publication trends in this field.Results: A total of 3467 articles were included. The number of publications were increasing globally every year. China made the most publications. But the USA made the highest contributions to the global research with the most citations and the highest H-index. CLINICAL ORTHOPAEDICS AND RELATED RESEARCH had the highest publication number. Studies could be classified into four clusters: process and clinical treatment, risk factors and diagnosis, pathophysiology, and basic research. Pathophysiology and basic research were predicted as the next hot topics in joint preserving procedures of ONFH.Conclusion: Base on the current global trends, it has been growing in a number of publications of joint preserving procedures of ONFH since 1999. The USA was the leading country in global research in this field. Pathophysiology and basic research may be the next hot spots, which need more attention in the future.
Generation and Evaluation of Chicken IgY-scFv for the Purposes of CPV Diagnosis and Therapy
Authors: Ge Shikun , Xu Long , Li Ben , Zhong Fagang , Zhang XiaoYing 
Published: May 2020

Abstract
Background: Canine parvovirus (CPV) can cause acute and highly contagious enteritis in dog, antibodies have been used for diagnosis and therapy of CPV disease, generation of functional antibody fragments by using novel antibody engineering platforms is promising in veterinary practice. Results: The IgY single chain fragment variables (scFv) were generated by T7 phage display system and expressed in E. coli system after immunizing hens with virus-like particles (VLP) of CPV-VP2. The titer of the primary scFv library reached to 1.5×10 6 pfu/mL, and 95% of the phages contained the target fragments. The CPV-VLP and CPV-VP2 protein showed similar reaction values to the purified scFv in the ELISA test, and the results of ELISA analysis using IgY-scFv toward CPV clinical samples were consistent with commercial immunochromatographic assay (ICA) and PCR detection, the scFv did not show cross reactivity with canine distemper virus (CDV) and canine coronavirus (CCV). IgY-scFv was successfully expressed in CRFK cells, and in the virus suppression assay, 55% of CPV infections were eliminated within 24 hours. Docking results demonstrated that the number of amino acids of the binding sides between scFv and VP2 were AA37 and AA40, respectively. Conclusions: This study revealed the feasibility of a novel functional antibody fragment development strategy by generating diversified avian IgY-scFv libraries towards the pathogenic target of interest for both detection and therapeutic purposes in veterinary medicine.