bluuebunny/crossref_metadata_2025_split huggingface dataset:
Different Subtypes of Collateral Vessels in Hemorrhagic Moyamoya Disease with p.R4810K Variant
Authors: Ge Peicong , Zhang Qian , Ye Xun , Liu Xingju , Deng Xiaofeng , Wang Jia , Wang Rong , Zhang Yan , Zhang Dong , Zhao Jizong 
Published: May 2020

Abstract
Background The aim of this study was to investigate the hemorrhgic sites and collateral vessels in hemorrhagic MMD with the p.R4810K variant.Methods Hemorrhage sites were classified as either anterior or posterior. Collateral vessels were classified into three subtypes according to origin: lenticulostriate anastomosis, thalamic anastomosis, and choroidal anastomosis. Lenticulostriate anastomosis. Hemorrhage sites and collateral vessels were compared between patients with wild-type p.R4810K variant (GG) and patients with heterozygous p.R4810K variant (GA) after 1:1 propensity score matching.Results A total of 130 hemorrhagic MMD patients were included in present study, 21 pairs (42 hemorrhagic hemispheres) were obtained after 1:1 propensity score. In GA group, 16 hemispheres (76.2%) presented anterior hemorrhage, and 5 hemispheres (23.8%) presented with posterior hemorrhage. In GG group, 13 hemispheres (61.9%) presented anterior hemorrhage, and 8 hemispheres (38.1%) presented with posterior hemorrhage. No significant differences were found in hemorrhagic sites between two matched groups (P > 0.05). Of 21 hemispheres in GA group, 10 (47.6%) exhibited lenticulostriate anastomosis, 6 (28.6%) thalamic anastomosis, and 6 (28.6%) choroidal anastomosis. Of 21 hemispheres in GG group, 3 (14.3%) exhibited lenticulostriate anastomosis, 5 (23.8%) thalamic anastomosis, and 9 (42.9%) choroidal anastomosis. There was significant difference in lenticulostriate anastomosis between two matched groups (P = 0.045). After adjustment the age, sex, and PCA involvement, we found that lenticulostriate anastomosis was associated with p.R4810K variant (OR, 5.995; 95% CI, 1.296–27.737; P = 0.022).Conclusion Lenticulostriate anastomosis were associated with p.R4810K variant. Whereas hemorrhagic sites, thalamic anastomosis, and choroidal anastomosis were not correlated with p.R4810K variant.
Diagnostic Accuracy of D-Dimer in Periprosthetic Joint Infection: a diagnostic meta-analysis
Authors: Zhang Haitao , Sun Xiaobo , Xin Pengfei , Zhu Xingyang , Jie Ke , Cao Houran , Feng Wenjun , Zeng Yuqing , Lv Yan , Chen Jinlun , LI Jie , Zeng Jianchun , zeng yirong 
Published: May 2020

Abstract
Background: Periprosthetic joint infection (PJI) is one of the most devastating complications after total joint replacement. Up to now, the diagnosis of PJI is still in a dilemma. As a novel biomarker, whether D-Dimer is valuable in the diagnosis of PJI remains controversial. This meta-analysis attempts to determine the diagnostic accuracy of D-Dimer in PJI.Methods: Relevant literature was retrieved from PubMed, Embase, Web of Science, and Cochrane Library (from database establishment to April 2020). Literature quality was evaluated using Revman (Version 5.3). The random effect model was used in Stata version 14.0 software to combine sensitivity, specificity, likelihood ratio (LR), diagnostic odds ratio (DOR), summary receiver operating characteristic (SROC) curve and area under SROC (AUC) to evaluate the diagnostic value of overall D-Dimer for PJI. Meta regression and subgroup analysis were performed according to the threshold, the study design, the sample size, the diagnostic gold standard, the country of study, and the type of sample.Results:A total of 9 studies were included in this study, including 1592 patients. The pooled sensitivity and specificity of D-Dimer for PJI diagnosis are 0.82(95%CI, 0.72~0.89) and 0.73(95%CI, 0.58~0.83), respectively. The pooled positive likelihood ratio (PLR) and negative likelihood ratio (NLR) were 2.99 (95%CI, 1.84~4.88) and 0.25 (95%CI, 0.15~0.41), respectively. The pooled AUC and diagnostic odds ratios were 0.85 (95%CI, 0.82~0.88) and 12.20 (95% CI, 4.98~29.86), respectively.Conclusion: D-Dimer is a promising biomarker for the diagnosis of PJI, which should be used in conjunction with other biomarkers or as an adjunct to other diagnostic methods to enhance diagnostic performance.
Elevated Serum Free Carnitine Levels in Children with Kawasaki Disease and Their Relation to Unresponsiveness to Intravenous Immunoglobulin: retprospective study
Authors: Muto Taichiro , Nakamura Nami , Masuda Yu , Numoto Shingo , Kodama Shunsuke , Miyamoto Ryosuke , Miyata Kenji , Hayakawa Tomohito , Mori Hiromitsu , Kuroyanagi Yoshiyuki , Akaihata Mitsuko , Iwayama Hideyuki , Kurahashi Hirokazu , Shimomura Yasuhito , Nagai Takuhito , Hori Toshinori , Agata Hiroatsu , Okumura Akihisa 
Published: May 2020

Abstract
Background

Carnitine plays an essential role in transfer of long-chain fatty acids to mitochondria for subsequent β-oxidation. No studies to date have characterized carnitine in children with KD. The objective of this study is to investigate the characteristics of serum free carnitine (FC) in hospitalized pediatric patients with Kawasaki disease (KD).
Methods

In total, 45 patients with KD measured the levels of serum FC from October 2018 to December 2019 were analyzed retrospectively. We analyzed the clinical and laboratory parameters just before the Intravenous immunoglobulin (IVIG) including serum levels of serum FC with respect to the IVIG response.
Results

The median age was 33 months. IVIG was effective in 33 children (responders) and was ineffective in 12 (non-responders). The serum FC levels were higher in non-responders than in responders [(35.3 µmol/L (range, 26.8-118.4 µmol/L) vs. 31.4 µmol/L (range, 20.9–81.2 µmol/L), p value = 0.0496]. The FC levels before intravenous immunoglobulin (IVIG) in four-fifths of responders were below the normal range.The levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), total bilirubin, and FC were higher in non-responders than in responders. There was a correlation between FC and AST (R2 = 0.364, P = 0.0015) and between FC and ALT (R2 = 0.423, P < 0.001) levels.
Conclusion

FC levels were upregulated in patients with KD who were refractory to IVIG. Additionally, FC levels in children with KD correlated with AST and ALT levels. The pathogenesis resulting in the elevation of FC levels remains elusive. Further studies are necessary to understand more precisely carnitine properties in patients with KD.
High conservation combined with high plasticity: genomics and evolution of Borrelia bavariensis
Authors: Becker Noémie S , Rollins Robert Ethan , Nosenko Kateryna , Paulus Alexander , Martin Samantha , Krebs Stefan , Takano Ai , Sato Kozue , Kovalev Sergey Y , Kawabata Hiroki , Fingerle Volker , Margos Gabriele 
Published: May 2020

Abstract
BackgroundBorrelia bavariensis is one of the agents of Lyme Borreliosis (or Lyme disease) in Eurasia. The genome of the Borrelia burgdorferi sensu lato species complex, that includes B. bavariensis , is known to be very complex and fragmented making the assembly of whole genomes with next-generation sequencing data a challenge. ResultsWe present a genome reconstruction for 33 B. bavariensis isolates from Eurasia based on long-read (Pacific Bioscience, for three isolates) and short-read (Illumina) data. We show that the combination of both sequencing techniques allows proper genome reconstruction of all plasmids in most cases but us e of a very close reference is necessary when only short-read sequencing data is available. B. bavariensis genomes combine a high degree of genetic conservation with high plasticity: all isolates share the main chromosome and five plasmids, but the repertoire of other plasmids is highly variable. In addition to plasmid losses and gains through horizontal transfer, we also observe several fusions between plasmids. Although European isolates of B. bavariensis have little diversity in genome content, there is some geographic structure to this variation. In contrast, each Asian isolate has a unique plasmid repertoire and we observe no geographically based differences between Japanese and Russian isolates. Comparing the genomes of Asian and European populations of B. bavariensis suggest s that some genes which are markedly different between the two populations may be good candidates for adaptation to the tick vector, ( Ixodes ricinus in Europe and I. persulcatus in Asia) . ConclusionsWe present the characterization of genomes of a large sample of B. bavariensis isolates and show that their plasmid content is highly variable. This study opens the way for genomic studies seeking to understand host and vector adaptation as well as human pathogenicity in Eurasian Lyme borreliosis agents.
Nucleotide diversity of functionally different groups of immune response genes in Old World camels based on newly annotated and reference-guided assemblies
Authors: Lado Sara , Elbers Jean P. , Rogers Mark F. , Melo-Ferreira José , Yadamsuren Adiya , Corander Jukka , Horín Petr , Burger Pamela A. 
Published: May 2020

Abstract
The preprint has been removed because it was posted in error by Research Square.
Recurrence of Hepatitis C Virus after treatment with Pegylated Interferon and Direct Acting Antivirals in Punjab, Pakistan.
Authors: Raza Muhammad Naeem , Sughra Kalsoom , Zeeshan Nadia 
Published: May 2020

Abstract
Background Although increased response rates concomitant in hepatitis C virus but relapse after treatment is threatened. Therefore, it is terrible requirement to evaluate the response of Pegylated interferon and direct acting antivirals in Pakistan.Methods This study conducted at Department of Pathology, Nawaz Sharif Medical College Gujrat, while treatment effects monitored in different Government and Private Hospitals of Punjab, Pakistan from July 2017 to January 2019. Total 973 patients administered the recommended dose that divided in two groups (i) Interferon based therapy (ii) direct acting antivirals (DAAs).Other parameters like ALT and viral load studied.Results The 374 patients given interferon therapy and 32 of 374 were positive after 24 weeks of treatment. Among these 29 patients have same genotype and recurrence was present .While 3 patients were re-infected with different HCV strains. In case of DAAs, only 27 patients were positive among 558 patients after 2 weeks and only one patient re-infected with different genotype. Early and sustained virologic response noted in DAAs. ALT and viral load decreased faster with DAAs that not achieved after 4 weeks with pegylated interferon.Conclusion SVR appears in DAAs and recurrence rate was high in interferon-based therapy as compared to DAAs. Therefore, reinfection has implications for correct treatment efficiency and to select optimal strategies for retreatment cases.
SARS-CoV-2 Origins and Evolution: Insights from Coronaviruses Recombination and Phylogenetic Analysis
Authors: Murahwa Alltalents Tutsirayi , Onywera Harris , Nindo Fredrick 
Published: May 2020

Abstract
Background: It is imperative in the midst of a global epidemic to investigate the origins of the infectious agent especially when it has reached parts of the world with either ailing economies or pre-existing political turmoil consistent with non-functional health systems. Methods: To explore the possibility of cross species infection, genomic recombination and the emergence of novel coronaviruses in the near future we carried out recombination and phylogenetic analysis to determine the spatio-temporal evolution and origins of the current SARS-CoV-2 virus. Results: Our findings prove using two robust recombination tools, RDPv4.100 and SimPlot3.5.1 analysis that SARS-CoV-2 is a recombinant of pangolin and bat RaTG13 sequences as been previously shown elsewhere. We also report one novel recombinantion event between two SARS-CoV-2 sequences ( SARS-CoV-2 sequence, MT188341 , SARS-CoV-2 sequence, MT293183). Bearing in mind that the prerequisite for recombination is the occurrence two viral sequences in the same reservoir, biological niche or host at the same time we postulate either co-infection with the two viral sequences, or superinfection, both scenarios have not been reported elsewhere. Conclusion: The possibility of recombination between the SARS-CoV-2 sequences poses the likelihood of the emergence of new and maybe more or less virulent “strains” of the virus. We believe that the future of science lies in our ability to be able to use computational based methods to predict the genetic sequences of infectious agents of the next epidemics. The addition of more SARS-CoV-2 sequences has a bearing on the understanding of the origin, evolution and clinical outcome prediction of given viral genomes. More SARS-CoV-2 sequences are needed to elucidate our understanding of this family of viruses.
Surgery Improve the Prognosis of Colon Mucinous Adenocarcinoma with Liver Metastases: A SEER-Based Study
Authors: Huang Jia , Chen Guodong , Liu Huan , Zhang Yiwei , Tang Rong , Huang Qiulin , Fu Kai , Peng Xiuda , Xiao Shuai 
Published: May 2020

Abstract
Background Mucinous adenocarcinoma (MAC) is the second common pathological type of colon carcinoma (CC). Colon cancer liver metastases (CLM) is common and lethal, complete resection for both of the primary tumor and metastases of CLM would be beneficial. However, there is still no consensus for the role of surgery in MAC of CC with liver metastases (M-CLM).Methods Among 5816 patients who diagnosed with M-CLM or classical adenocarcinoma of CLM (A-CLM) from 2010 to 2013 in the Surveillance, Epidemiology, and End Results (SEER) database were retrieved. The clinicopathological features and overall survival (OS) and cancer-specific survival (CSS) data were compared and analyzed.Results Total of 5816 M-CLM and A-CLM patients were enrolled in this study. Results showed M-CLM group had larger tumor size, more right colon location, high pT and pN stage compared with A-CLM group, as well as more female patients, more examined and positive lymph nodes and a higher proportion of surgery than A-CLM group. The OS and CSS of M-CLM patients accepted any surgery were significantly better than that didn’t accept any surgery, but poorer than that of A-CLM patients. Meanwhile, the OS and CSS of M-CLM and A-CLM were comparable when they didn’t receive any surgery. Moreover, partial colectomy provided the similar OS and CSS compared with hemicolectomy or greater for M-CLM, and surgery was an independent protective factor for long-term survival of M-CLM.Conclusions M-CLM had distinct clinicopathological characteristics, surgery could improve the long-term survival and act as an independent protective prognostic factor for M-CLM, additionally, partial colectomy might be a better selection for M-CLM from this study.
Investigation on Yield Behavior of 7075-T6 Aluminum Alloy at Elevated Temperatures
Authors: Lin Jianping , Bao Xingyu , Hou Yong , Min Junying , Qu Xinlei , Tao Zhimin , Chen Jiajie 
Published: May 2020

Abstract
Aluminum alloys have drawn considerable attention in the area of automotive lightweight. High strength aluminum alloys are usually deformed at elevated temperatures due to their poor formability at room temperature. In this work, the yield behavior of 7075 aluminum alloy in T6 temper (AA7075-T6) within the temperature ranging from 20 ℃ to 230 ℃ was investigated. Uniaxial and biaxial tensile tests with the aid of induction heating system were performed to determine the stress vs. strain curves and the yield loci of AA7075-T6 at elevated temperatures, respectively. Von Mises, Hill48 and Yld2000-2d yield criteria were applied to predicting yield loci which were compared with experimentally measured yield loci of the AA7075-T6. Results show that yield stress corresponding to the same equivalent plastic strain decreases with increasing temperature within the investigated temperature range and the shape of yield loci evolves nearly negligibly. The experimental yield locus expands with an increase of equivalent plastic strain at the same temperature and the work hardening rate of AA7075-T6 exhibits obvious stress-state-dependency. The non-quadratic Yld2000-2d yield criterion describes the yield surfaces of AA7075-T6 more accurately than the quadratic von Mises and Hill48 yield criteria, and an exponent of 14 in the Yld2000-2d yield function gives the optimal predictions for the AA7075-T6 at all investigated temperatures.
Definitive radiotherapy in the management of non-resectable or residual retroperitoneal sarcomas: institutional cohort analysis and systematic review
Authors: Sobiborowicz Aleksandra , Spałek Mateusz Jacek , Czarnecka Anna , Rutkowski Piotr 
Published: May 2020

Abstract
Background There is currently no consensus on optimal management of patients with primary or recurrent non-resectable/residual retroperitoneal sarcomas (RPS). The objective of this study was to document the outcomes of patients with primary or recurrent non-resectable/residual RPS treated in our center with definitive radiotherapy (RT), and to perform systematic review on that topic. Methods A retrospective analysis of consecutive RPS patients treated in our center between 2000 and 2019 was performed. All consecutive patients who underwent definitive conformal RT with image guidance for primary or recurrent non-resectable or macroscopically residual RPS were included. Additionally, the systematic review compliant with the recommendations of the Preferred Reporting Items for Systematic Reviews and Meta-Analyses was performed.Results 14 patients who met aforementioned criteria were found. Data on clinicopathological characteristics, RT and response to treatment were presented. RT allowed achieving prolonged local control of the disease i.e. no local progression of the disease for more than six months after RT in 12 patients. Achieved local control lasted more than 24 months in six cases, with none or minimal toxicity. 11 studies were included in the systematic review. Our results are in concordance to reports included in the review.Conclusions RT provided satisfactory local disease control with acceptable treatment tolerance in patients with primary or recurrent non-resectable/residual RPS. RT represents valuable treatment modality in this selected group of patients. Additional RT modalities i.e. particle therapy, MRI-guided RT or GRID/Lattice RT may be introduced to improve local control and minimize toxicity.
Characteristics and outcomes of Stanford type A aortic dissection patients with post-operation severe hyperbilirubinemia: a retrospective cohort study
Authors: Chen Xiaolan , Bai Ming , Zhao Lijuan , Li Yangping , Yu Yan , Zhang Wei , Ma Feng , Sun Shiren , Chen Xiangmei 
Published: May 2020

Abstract
Background
Hyperbilirubinemia is one of the common complications after cardiac surgery and is associated with increased mortality. However, to the best of our knowledge, the report on clinical significance of postoperative severe hyperbilirubinemia in Stanford type A aortic dissection (AAD) patients is limited.
Methods
Patients who underwent surgical treatment for AAD in our center between January 2015 and December 2018 were retrospectively screened. In-hospital mortality, long-term mortality, acute kidney injury (AKI), and the requirement of continuous renal replacement therapy (CRRT) were assessed as endpoints. Univariate and multivariate regression models were employed to identify the risk factors of these endpoints.
Results
271 (12.3%) patients were included. Of the included patients, 222 (81.9%) experienced postoperative AKI, and 50 (18.5%) received CRRT. In-hospital mortality was 30.3%. The 1-year, 2-year, and 3-year cumulative mortality were 32.9%, 33.9%, and 35.3%, respectively. Multivariate Logistic regression analysis indicated that age (P < 0.033), AKI stage 3 (P < 0.001), the amount of blood transfusion after surgery (P = 0.019), mean arterial pressure (MAP) in the first postoperative day (P = 0.012), the use of extracorporeal membrane oxygenation (ECMO) (P = 0.02), and the peak total bilirubin (TB) concentration (P = 0.023) were independent risk factors of in-hospital mortality. The optimal cut-off value of peak TB on predicting in-hospital mortality was 121.2 µmol/l. Survival analysis showed significantly decreased survival for patients who developed severe, rather than mild, hyperbilirubinemia.
Conclusions
Post-operation severe hyperbilirubinemia is a common clinical presentation in AAD surgery patients. Post-operation severe hyperbilirubinemia AAD patients with older age, lower MAP, increased blood transfusion, stage 3 AKI, the use of ECMO, and the increased peak TB had higher risk of in-hospital mortality.
A Universal Model for Prediction of COVID-19&nbsp;Pandemic&nbsp;Based on Machine Learning
Authors: Wang Lu , Han De-wei , Li Kang , Yang Xu , Yin Xiao-lei , Qiu Jing , Liang Dong-xue , Ma Zhao-yuan 
Published: May 2020

Abstract
Background: With the current worldwide spreading of the coronary virus (COVID-19) pandemic, accurately predicting the rate of spread of the virus has become an urgent need. Methods: In this article we propose a universal COVID-19 prediction model that is independent of country-specific factors in this paper. By analyzing the pandemic data in China, we combined the advantages of Gaussian function with that of chi-square distribution function, to render an innovative mathematical model named the H-Gaussian with five parameters to be learned, and solved the parameters by a gradient descent algorithm. Results: We trained the model with partial historical pandemic data to predict subsequent pandemic trends in several regions, and validated the predictions with real data. The H-Gaussian model was experimentally shown to correctly predict the pandemic trends, and the parameters had good interpretability. Conclusions: On this basis, the global trends of the pandemic are given based on the data currently available, as well as suggestions for subsequent prevention strategies.