bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of Candidate Genetic Markers and a Novel 4-genes Diagnostic Model in Osteoarthritis through Integrating Multiple Microarray Data
Authors: Jiang Ai , Xu Peng , Zhao Zhenda , Tan Qizhao , Sun Shang , Song Chunli , Leng Huijie 
Published: April 2020

Background:
Osteoarthritis (OA) is a joint disease that leads to a high disability rate
and a low quality of life. With the development of modern molecular biology techniques, some key
genes and diagnostic markers have been reported. However, the etiology and pathogenesis of OA
are still unknown.

Objective:
To develop a gene signature in OA.

Method:
In this study, five microarray data sets were integrated to conduct a comprehensive
network and pathway analysis of the biological functions of OA related genes, which can provide
valuable information and further explore the etiology and pathogenesis of OA.

Results and Discussion:
Differential expression analysis identified 180 genes with significantly
expressed expression in OA. Functional enrichment analysis showed that the up-regulated genes
were associated with rheumatoid arthritis (p < 0.01). Down-regulated genes regulate the biological
processes of negative regulation of kinase activity and some signaling pathways such as MAPK
signaling pathway (p < 0.001) and IL-17 signaling pathway (p < 0.001). In addition, the OA
specific protein-protein interaction (PPI) network was constructed based on the differentially
expressed genes. The analysis of network topological attributes showed that differentially upregulated
VEGFA, MYC, ATF3 and JUN genes were hub genes of the network, which may
influence the occurrence and development of OA through regulating cell cycle or apoptosis, and
were potential biomarkers of OA. Finally, the support vector machine (SVM) method was used to
establish the diagnosis model of OA, which not only had excellent predictive power in internal and
external data sets (AUC > 0.9), but also had high predictive performance in different chip
platforms (AUC > 0.9) and also had effective ability in blood samples (AUC > 0.8).

Conclusion:
The 4-genes diagnostic model may be of great help to the early diagnosis and
prediction of OA.
5Ps STRATEGY FOR COAL MINING GOVERNANCE IN THE PERSPECTIIVE OF SDGs IN SOUTH MALINAU, NORTH KALIMANTAN
Authors: Labo Tomy , Setyowati Endah , Domai Tjahjanulin 
Published: April 2020

AbstractÂ The objective of the study is to construct 5Ps strategy for coal mining governance using the perspective of SDGs in the Malinau District of North Kalimantan, Indonesia. The study was conducted using qualitative method. Data was collected through field observations and in-depth interviews, and secondary data of official published documents. Data was analyzed using the interactive analysis Model of Mile, Huberman, and Saldana. This research concluded that the 5Ps strategy for coal mining productions in the Malinau District of North Kalimantan is very useful for governing all stakeholders in the District in respect to environmental, social and economic aspects. The 5Ps strategy is 1) People, this means communities should be involved in the coal mining governance, 2) Planet, environmental awareness, 3) Profit, economic aspects should be well calculated and managed, 4) Peace, justice and equality are also the spirit to manage the coal productions and colaborations among the stakeholders are important to make the success of production.Keywords: Coal Mining Governance, Sustainable Development Goals, 5Ps Strategy
Users’ Experiences of a Mobile Health Self-Management Approach for the Treatment of Cystic Fibrosis: Mixed Methods Study
Authors: Floch Jacqueline , Vilarinho Thomas , Zettl Annabel , Ibanez-Sanchez Gema , Calvo-Lerma Joaquim , Stav Erlend , Haro Peter Halland , Aalberg Asbjørn Lein , Fides-Valero Alvaro , Bayo Montón José Luis 
Published: April 2020

Background
Despite a large number of clinical trials aiming at evaluating the digital self-management of chronic diseases, there is little discussion about users’ experiences with digital approaches. However, a good user experience is a critical factor for technology adoption. Understanding users’ experiences can inform the design of approaches toward increased motivation for digital self-management.

Objective
This study aimed to evaluate the self-management of cystic fibrosis (CF) with a focus on gastrointestinal concerns and the care of young patients. Following a user-centered design approach, we developed a self-management app for patients and parents and a web tool for health care professionals (HCPs). To evaluate the proposed solutions, a 6-month clinical trial was conducted in 6 European CF competence centers. This paper analyzes the user acceptance of the technology and the benefits and disadvantages perceived by the trial participants.

Methods
A mixed methods approach was applied. Data were collected through 41 semistructured qualitative interviews of patients, parents, and HCPs involved in the clinical trial. In addition, data were collected through questionnaires embedded in the self-management app.

Results
Support for enzyme dose calculation and nutrition management was found to be particularly useful. Patients and parents rapidly strengthened their knowledge about the treatment and increased their self-efficacy. Reported benefits include reduced occurrence of symptoms and enhanced quality of life. Patients and parents had different skills, requiring follow-up by HCPs in an introductory phase. HCPs valued obtaining precise information about the patients, allowing for more personalized advice. However, the tight follow-up of several patients led to an increased workload. Over time, as patient self-efficacy increased, patient motivation for using the app decreased and the quality of the reported data was reduced.

Conclusions
Self-management enfolds a collaboration between patients and HCPs. To be successful, a self-management approach should be accepted by both parties. Through understanding behaviors and experiences, this study defines recommendations for a complex case—the demanding treatment of CF. We identify target patient groups and situations for which the app is most beneficial and suggest focusing on these rather than motivating for regular app usage over a long time. We also advise the personalized supervision of patients during the introduction of the approach. Finally, we propose to develop guidance for HCPs to facilitate changes in practice. As personalization and technology literacy are factors found to influence the acceptance of digital self-management of other chronic diseases, it is relevant to consider the proposed recommendations beyond the case of CF.
The Influence of Electronic Health Record Use on Physician Burnout: Cross-Sectional Survey
Authors: Tajirian Tania , Stergiopoulos Vicky , Strudwick Gillian , Sequeira Lydia , Sanches Marcos , Kemp Jessica , Ramamoorthi Karishini , Zhang Timothy , Jankowicz Damian 
Published: April 2020

Background
Physician burnout has a direct impact on the delivery of high-quality health care, with health information technology tools such as electronic health records (EHRs) adding to the burden of practice inefficiencies.

Objective
The aim of this study was to determine the extent of burnout among physicians and learners (residents and fellows); identify significant EHR-related contributors of physician burnout; and explore the differences between physicians and learners with regard to EHR-related factors such as time spent in EHR, documentation styles, proficiency, training, and perceived usefulness. In addition, the study aimed to address gaps in the EHR-related burnout research methodologies by determining physicians’ patterns of EHR use through usage logs.

Methods
This study used a cross-sectional survey methodology and a review of administrative data for back-end log measures of survey respondents’ EHR use, which was conducted at a large Canadian academic mental health hospital. Chi-square and Fisher exact tests were used to examine the association of EHR-related factors with general physician burnout. The survey was sent out to 474 individuals between May and June 2019, including physicians (n=407), residents (n=53), and fellows (n=14), and we measured physician burnout and perceptions of EHR stressors (along with demographic and practice characteristics).

Results
Our survey included 208 respondents, including physicians (n=176) and learners (n=32). The response rate was 43.2% for physicians (full-time: 156/208, 75.0%; part-time: 20/199, 10.1%), and 48% (32/67) for learners. A total of 25.6% (45/176) of practicing physicians and 19% (6/32) of learners reported having one or more symptoms of burnout, and 74.5% (155/208) of all respondents who reported burnout symptoms identified the EHR as a contributor. Lower satisfaction and higher frustration with the EHRs were significantly associated with perceptions of EHR contributing toward burnout. Physicians’ and learners’ experiences with the EHR, gathered through open-ended survey responses, identified challenges around the intuitiveness and usability of the technology as well as workflow issues. Metrics gathered from back-end usage logs demonstrated a 13.6-min overestimation in time spent on EHRs per patient and a 5.63-hour overestimation of after-hours EHR time, when compared with self-reported survey data.

Conclusions
This study suggests that the use of EHRs is a perceived contributor to physician burnout. There should be a focus on combating physician burnout by reducing the unnecessary administrative burdens of EHRs through efficient implementation of systems and effective postimplementation strategies.
Cognitive Behavior Therapy dalam menangani kecemasan sosial pada remaja
Authors: Rahmadiani Nixie Devina 
Published: April 2020

Remaja dengan kecemasan sosial ditandai dengan perasaan ketidaknyamanan yang signifikan dan menghindari situasi sosial. Individu mudah merasa gugup ketika berada di lingkungan ramai, terkadang muncul keringat dingin, merasa cemas apabila berjalan sendiri sehingga lebih sering pergi ditemani orang lain. Apakah Intervnsi Cognitive Behavior Therapy efektif dalam menangani kecemasan sosial? Studi kasus ini melibatkan seorag remaja yang mengalami kecemasan social yang merasa tidak aman dan cenderung bersikap malu-malu dalam bersikap. Asesmen dilakukan dengan observasi, wawancara, tes psikologi (CPM, FSCT dan grafis), dan skala kecemasan sosial Liebowitz. Intervensi Cognitive BehaviorTherapy dilaksanakan selama 9 sesi. Hasil dari intervensi menunjukkan subjek mampu mengidentifikasi pemikiran negatif dan merubah menjadi lebih positif serta menurunnya tingkat kecemasan subjek saat berjalan sendiri di lingkungan ramai dalam setting sekolah sesuai target intervensi yang telah ditentukan. Keywords: Cognitive Behavior Therapy, kecemasan sosial, remaja, tes psikologi.
Bioactive Constituent Profiling of Poly Herbal Distillate Thiribhaladi Dravagam to Evaluate Its Credible Therapeutic Role in Siddha Clinical Practices
Authors: S Vinayak , R Gayatri , Wilson Eugene , Rajkumar Shyamala , Kumar K.N Sunil , Selvarajan S. , K Kanakavalli 
Published: April 2020

Introduction: Hydro distillation in Siddha medicine is the technique of extracting valuable principles from a pre-processed raw drug through the traditional process of distillation under a controlled temperature setting. Thiribhaladi Dravagam (TD) is one of the polyherbal distillate formulation stated in the Tamil medical text specified for phlegmatic affections. The drug was subjected to GC-MS studies to screen its bioactive sketch. Methods: Two sets of raw drug samples from different locations were collected. The raw drugs were purified and pre-processed as per the classical literature and distilled in a traditional apparatus. Two distillate samples (TD 1 & TD 2) were prepared from the set of ingredients and were screened for its constituents. Results: The Gas chromatogram of TD 1 revealed 4 prominent peaks with retention time ranging from 4.84 to 8.27. TD 2 reported 8 prominent peaks with retention time ranging from 3.35 to 7.88. The study testified the presence of active volatile metabolites like aldehydes and oxygenated monoterpenes like citral and borneol, monocyclic alcoholic compounds like terpineol and organic acids like caprylic acid from both the samples. TD 2 reported more pharmacologically valuable lead molecules than TD 1, which may be due to the variation in diversity of the two sets of raw drug samples. Conclusion: The bio constituents of TD was found to be pharmacologically significant from the review of various reputed research works supporting the traditional claim on its role in the respiratory system and for maintaining good health. Through the review of Thiribhaladi Dravagam and its analytical part performed through GC-MS, the medical applications of TD in multiple clinical associations and as a perfect health promoter has been justified for promoting its traditional value.
The Rationality of the Use of Intravenous Preparations in Diarrhea Patients in the Tk Ii Putri Hijau Kesdam I Bukit Barisan Hospital
Authors: D Darmayanto , W Wiryanto , Suwarso Edy 
Published: April 2020

Objective: to evaluate the rationality of the use of intravenous preparations during diarrhea therapy which is limited to the right patient, the right indication, the right drug and the right dose.
Design: the study used descriptive and retrospective design by medical record of the TK II Putri Hijau KESDAM I Bukit Barisan hospital Medan. The criteria taken are patients with diarrhea with bacterial infections in the period of January – December 2018.
Interventions: the intervened variable were the rationality of the use of intravenous preparations during diarrhea with bacterial infections.
Main outcome measures: the main measurement in this study were the right patient, the right indication, the right drug and the right dose for diarrhea patients with bacterial infections..
Results: there were 885 diarrhea patients included in the inclusion criteria out of a total of 1,302 patients. Inaccurated of drugs used during diarrhea therapy were ceftriaxonee that overdosed in 36 cases and ciprofloxacin that overdosed by 14 cases.
Conclusion: it could be concluded that the inaccurate use of ceftriaxonee and ciprofloxacin were included in the incorrect criteria of the right dose for patients with diarrhea with bacterial infections.
Inhibitory AKT2 And Vegr2 Genes Expression of Avicennia Marina Using RT-PCR On Widr Cells
Authors: T Qurrohman, , P.A.Z. Hasibuan, , M Basyuni, 
Published: April 2020

Objectives: To evaluate the activities the anticancer of n-hexane extract of Avicennia marina leaves on WiDr cells in down-regulated of expression of Akt2 and VEGFR2 genes.
Methods: Avicennia marina leaves were dried and extracted with n-hexane, analyzed the down-regulated Akt 2 and VEGFR2 expression which was determined the Reverse Transcription Polymerase Chain Reaction (RT-PCR) method.
Results:N-hexane extract of Avicennia marina, in which there were down regulated expression Akt 2 and VEGFR2 of 0.43 and 0.50 WiDr cells. N-hexane extract of mangrove leaves has cancer chemoprevention activity with down-regulated on WiDr cells.
Conclusions: :N-hexane extract of Avicennia marina leaves had anticancer activity with down-regulated on WiDr cells, suggest that significantly inhibit the expression of Akt2 and VEGFR2 genes.
Outcome assessment of orthodontic clear aligner vs fixed appliance treatment in a teenage population with mild malocclusions
Authors: Borda Alissa F. , Garfinkle Judah S. , Covell David A. , Wang Mansen , Doyle Larry , Sedgley Christine M. 
Published: April 2020

ABSTRACT

Objective
To assess the efficacy and efficiency of treatment in adolescents presenting with mild malocclusions, comparing outcomes using clear aligners to fixed appliances.

Materials and Methods
Patients identified retrospectively and consecutively from one private practice had been treated with either clear aligners (Invisalign, Align Technology, Santa Clara, Calif) or fixed appliances (0.022 Damon, Ormco, Orange, Calif; n = 26/group). Assessments of occlusion were made using the American Board of Orthodontics Discrepancy Index (DI) for initial records and Cast-Radiograph Evaluation (CRE) for final records. Number of appointments, number of emergency visits, and overall treatment time were determined from chart reviews. Data were analyzed using Pearson's correlation, Wilcoxon rank tests, unpaired t-tests, and Chi-square tests, with significance set to P ≤ .05.

Results
Pretreatment, the aligner and fixed groups showed no significant difference in overall severity (DI: 11.9 ± 5.3 vs 11.6 ± 4.8) or in any individual DI category. Posttreatment scores showed finishes for the aligner group had fewer discrepancies from ideal relative to the fixed appliance group (CRE: 30.1 ± 8.3 vs 37.0 ± 9.3; P &lt; .01). Patients treated with aligners had fewer appointments (13.7 ± 4.4 vs 19.3 ± 3.6; P &lt; .0001), fewer emergency visits (0.8 ± 1.0 vs 3.6 ± 2.5; P &lt; .0001), and shorter overall treatment time (16.9 ± 5.7 vs 23.4 ± 4.4 months; P &lt; .0001).

Conclusions
Outcomes for treatment of mild malocclusions in adolescents showed equivalent effectiveness of clear aligners compared to fixed appliances, with significantly improved results for clear aligner treatment in terms of tooth alignment, occlusal relations, and overjet. Assessment of the number of appointments, number of emergency visits, and overall treatment time showed better outcomes for treatment with clear aligners.
The Molecular Diagnosis Protocols of New Coronavirus (COVID-19); Specificity and Sensitivity an Overview
Authors: Ahmed Hama Abdullah , Abdulrahman Mohammed Othman , Mahmud Ali Fatima , Hamid Shareef Osama , Muhammad Wli Sardar , Sharif Sabiha , Ahmed Qadir Syamand 
Published: April 2020

Acute respiratory tract infection is a common public health concern worldwide a new emerging contagious virus (COVID-2019) or SARSC- 2 causing a pandemic pneumonia outbreak, The main transmission route of this virus is through droplets from respiratory made during sneezing or coughing of infected people like the recent viral infection of severe acute respiratory syndrome (SARS-CoV1) and the Middle East respiratory syndrome (MERS). Many epidemiological factors have a crucial role in promoting the transmission of the COVID-2019 that makes the disease as an emerging and global alarming against this new coronavirus. Early diagnosis of the etiological agents is critical for appropriate management, controlling plan, protection, and treatment. The new outbreak of COVID-19 can be detected by different molecular protocols. Quantitative polymerase chain reaction (qPCR) is the recommended technique used with varied sensitivity due to primers variation and specimen type. The reliable, high specific and sensitive diagnosis protocols are necessary for an emerging control plan. This study will review and explore the most available methods of molecular identification and primers for the diagnosis of the new coronavirus (COVID-19). This review will also open the new clues to develop and select appropriate diagnosis panel and specific primers for new coronavirus. In conclusion of this review, the RNA dependent RNA polymerase (RdRp) and RdRp/Hel protocols will be valuable to distinguish the COVID-19 from the SARS-CoV and the other respiratory viral pathogens.
i-Sign: Sign Language Learning Application Via Gamification
Authors: Seman Fadhlina Izzah , Mhd Shariff Nor Fahira , Nasaruddin Nor Intan Shafini 
Published: April 2020

Hearing loss is a type of disability where people who suffer from it could not hear a sound or can only listen to a sound with certain levels. People with hearing loss use sign language to communicate with others and to help them in their learning process. For this study, an interview and survey were conducted with teachers and students from SK Pendidikan Khas Melaka and few problems have been identified in the learning spectrum of children with hearing disability. They face difficulties in learning due to the lack of exposure about the correct sign language since young, difficulty in interpreting sign language in the conventional teaching material due to the nature of its static image contents, and hearing loss children are also usually less focused in the learning process and are easily distracted by their surroundings when the class is in session. To solve these problems, i-Sign is developed as a learning application that incorporates simple gamification in its development that could help hearing loss children to learn the alphabet and basic words of sign language. The use of gamification and multimedia elements provide a more interactive and fun learning environment for the students instead of using static contents, and are also used to direct students’ attention to the learning activity making them more focused in the learning process. i-Sign was developed using the ADDIE model which includes the Analysis, Design, Development, Implementation, and Evaluation phases. In the evaluation phase, a usability test was conducted on 8 children with hearing loss to test the acceptance of the user towards this application and 91.89% of the respondents found that the application meets their expectation of the application capability in terms of its effectiveness, efficiency and user satisfaction. This shows that gamification can be used as an alternative learning material for hearing loss children and help expose the correct sign language to them. The application can also be used by the public that wants to learn about sign language.
Aspects of Wellness in a Traditional Leisure Time Game: An Integrated Approach for Teaching Innovation.
Authors: Mohamed Mawarni , Tajuddin Puteri Aquila 
Published: April 2020

Malaysia and ASEAN societies are rich with a variety of traditional games and usually played during leisure time. ‘Tossing the Stones’ or Batu Seremban was chosen in this survey. Using a purposive sampling technique, respondents from Malaysia, Philippines, Indonesia and Thailand went through the game intervention and answered the questionnaires provided. The goal was to understand how they perceived wellness while playing this game, after identifying the frequency of Traditional Games engagement. Results showed that Batu Seremban gave high wellness scores such as; M=4.16, SD=.722. The physical, intellectual, social and emotional elements identified in ‘Tossing the Stones’ showed that strong educational elements identified in the traditional leisure game can also be used to teach children and young adults through integration in the curriculum. Suggestions on how the game can be used in classrooms were made based on the findings.
KEYWORDS: Batu Seremban, Curriculum, Innovation, Leisure, Traditional game, Wellness
INVESTIGATING THE IMPORTANCE LEVEL AND UTILIZATION OF VOCABULARY LEARNING STRATEGIES AMONG TURKISH
Authors: Yolcu Melek , Mirioğlu Meryem 
Published: April 2020

This study aims to investigate (a) the perceptions of 8th grade Turkish EFL learners regarding the importance and use of L2 vocabulary learning strategies (VLS), (b) to find out the relationship between perceived importance level and application level of VLS and (c) to unearth the most and the least frequently applied VLS by EFL learners in their learning processes. A mixed-method sequential explanatory design was adopted in this study. The quantitative data were collected through a questionnaire based on Schmitt's taxonomy of VLS from 398 participants and analyzed via SPSS; qualitative data were obtained from focus group interviews conducted with 45 volunteer participants and analyzed through thematic analysis. The findings indicated that EFL learners attribute a great level of significance to vocabulary learning. It also revealed that there is a significant relationship between importance and application level of VLS, which demonstrates that the strategies that were given most importance were applied on a larger scale by learners.

Keywords: English Language Learning, Vocabulary Learning, Vocabulary Learning Strategies, Learner Perceptions.
NB: Please note that the first author is Melek YOLCU.