bluuebunny/crossref_metadata_2025_split huggingface dataset:
Preemptive interferon-α treatment could protect against relapse and improve long-term survival of ALL patients after allo-HSCT
Authors: Liu Sining , Luo Xueyi , Zhang Xiaohui , Xu Lanping , Wang Yu , Yan Chenhua , Chen Huan , Chen Yuhong , Han Wei , Wang Fengrong , Wang Jingzhi , Liu Kaiyan , Huang Xiaojun , Mo Xiaodong 
Published: November 2020

AbstractRelapse was the major cause of treatment failure in patients with acute lymphoblastic leukemia (ALL) after allogeneic hematopoietic stem cell transplantation (allo-HSCT). We aimed to identify the efficacy and safety of preemptive interferon-α (IFN-α) treatment in ALL patients who had minimal residual disease (MRD) after allo-HSCT. Multiparameter flow cytometry and polymerase chain reaction assays were applied for MRD monitoring. Recombinant human IFN-α-2b injections were administered subcutaneously twice weekly in every 4 weeks cycle. Twenty-four (35.3%), 5 (7.4%), 6 (8.8%), and 13 (19.1%) patients achieved MRD negativity at 1, 2, 3, and > 3 months, respectively, after treatment. Seven patients showed grade ≥ 3 toxicities after IFN-α treatment. The 4-year cumulative incidence of total acute graft-versus-host disease (aGVHD), severe aGVHD, total chronic GVHD (cGVHD), and severe cGVHD after treatment was 14.7%, 2.9%, 40.0%, and 7.5%, respectively. The 4-year cumulative incidences of relapse and non-relapse mortality after treatment was 31.9% and 6.0%, respectively. The 4-year probabilities of disease-free survival and overall survival after IFN-α treatment were 62.1% and 71.1%, respectively. Thus, preemptive IFN-α treatment could protect against relapse and improve long-term survival for ALL patients who had MRD after allo-HSCT. The study was registered athttps://clinicaltrials.govas #NCT02185261 (09/07/2014).
Ocular surface manifestation of COVID-19 and tear film analysis
Authors: Meduri Alessandro , Oliverio Giovanni William , Mancuso Giuseppe , Giuffrida Angela , Guarneri Claudio , Venanzi Rullo Emmanuele , Nunnari Giuseppe , Aragona Pasquale 
Published: November 2020

AbstractTo evaluate the ocular manifestation in patients hospitalized with coronavirus disease 2019 (COVID-19) and to search for the presence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in tears. This study was conducted in 29 hospitalized patients who were admitted to the COVID center at the Policlinic Hospital of the University of Messina, Italy. All patients underwent an ophthalmologic assessment comprising a Standardized Patient Evaluation of Eye Dryness (SPEED) questionnaire, anterior segment, and the ocular surface examination of both eyes using a portable slit lamp. The Schirmer I test was performed, and the filter paper strip was used to search for the presence of SARS-CoV-2 on the ocular surface by real-time quantitative polymerase chain reaction (RT-qPCR). A total of 10 patients reported ocular symptoms; in particular, four reported eye burning, three reported foreign body sensation, and three reported tearing. Moreover, seven patients presented conjunctival hyperemia and/or chemosis, eleven patients presented blepharitis signs such as lid margin hyperemia and/or telangiectasia, crusted eyelashes, and meibomian orifices alterations. Tear analysis did not reveal the presence of SARS-CoV-2. Ocular symptoms are common in patients with COVID-19; although, tear analysis did not reveal the presence of SARS-CoV-2.
In situ casting of rice husk ash in metal organic frameworks induces enhanced CO2 capture performance
Authors: Panda Debashis , Saini Chanchal , Kumar E. Anil , Singh Sanjay Kumar 
Published: November 2020

AbstractIncorporation of rice-husk-ash (RHA), an agricultural waste, in situ during the synthesis of MIL-101(Cr) resulted in a significant improvement in the CO2 adsorption properties over the synthesized RHA-MIL-101(Cr). The newly synthesized RHA-MIL-101(Cr) composite exhibited an enhancement of 14–27% in CO2 adsorption capacity as compared to MIL-101(Cr) at 25 °C and 1 bar. The content of RHA incorporated in RHA-MIL-101(Cr) fine tuned the CO2 capture performance to achieve high working capacity (0.54 mmol g−1), high purity (78%), superior CO2/N2 selectivity (18) and low isosteric heat of adsorption (20–30 kJ mol−1). The observed superior CO2 adsorption performance of RHA-MIL-101(Cr) is attributed to the fine tuning of textural characteristics—enhancement of 12–27% in BET surface area, 12–33% in total pore volume and 18–30% in micropore volume—upon incorporation of RHA in MIL-101(Cr).
Brassica oleracea var. acephala (kale) improvement by biological activity of root endophytic fungi
Authors: Poveda Jorge , Zabalgogeazcoa Iñigo , Soengas Pilar , Rodríguez Victor M. , Cartea M. Elena , Abilleira Rosaura , Velasco Pablo 
Published: November 2020

AbstractBrassica oleracea var. acephala (kale) is a cruciferous vegetable widely cultivated for its leaves and flower buds in Atlantic Europe and the Mediterranean area, being a food of great interest as a "superfood" today. Little has been studied about the diversity of endophytic fungi in the Brassica genus, and there are no studies regarding kale. In this study, we made a survey of the diversity of endophytic fungi present in the roots of six different Galician kale local populations. In addition, we investigated whether the presence of endophytes in the roots was beneficial to the plants in terms of growth, cold tolerance, or resistance to bacteria and insects. The fungal isolates obtained belonged to 33 different taxa. Among those, a Fusarium sp. and Pleosporales sp. A between Setophoma and Edenia (called as Setophoma/Edenia) were present in many plants of all five local populations, being possible components of a core kale microbiome. For the first time, several interactions between endophytic fungus and Brassica plants are described and is proved how different interactions are beneficial for the plant. Fusarium sp. and Pleosporales sp. B close to Pyrenophora (called as Pyrenophora) promoted plant growth and increased cold tolerance. On the other hand, isolates of Trichoderma sp., Pleosporales sp. C close to Phialocephala (called as Phialocephala), Fusarium sp., Curvularia sp., Setophoma/Edenia and Acrocalymma sp. were able to activate plant systemic resistance against the bacterial pathogen Xanthomonas campestris. We also observed that Fusarium sp., Curvularia sp. and Setophoma/Edenia confered resistance against Mamestra brassicae larvae.
Accuracy and efficiency of germline variant calling pipelines for human genome data
Authors: Zhao Sen , Agafonov Oleg , Azab Abdulrahman , Stokowy Tomasz , Hovig Eivind 
Published: November 2020

AbstractAdvances in next-generation sequencing technology have enabled whole genome sequencing (WGS) to be widely used for identification of causal variants in a spectrum of genetic-related disorders, and provided new insight into how genetic polymorphisms affect disease phenotypes. The development of different bioinformatics pipelines has continuously improved the variant analysis of WGS data. However, there is a necessity for a systematic performance comparison of these pipelines to provide guidance on the application of WGS-based scientific and clinical genomics. In this study, we evaluated the performance of three variant calling pipelines (GATK, DRAGEN and DeepVariant) using the Genome in a Bottle Consortium, “synthetic-diploid” and simulated WGS datasets. DRAGEN and DeepVariant show better accuracy in SNP and indel calling, with no significant differences in their F1-score. DRAGEN platform offers accuracy, flexibility and a highly-efficient execution speed, and therefore superior performance in the analysis of WGS data on a large scale. The combination of DRAGEN and DeepVariant also suggests a good balance of accuracy and efficiency as an alternative solution for germline variant detection in further applications. Our results facilitate the standardization of benchmarking analysis of bioinformatics pipelines for reliable variant detection, which is critical in genetics-based medical research and clinical applications.
Maternal plasma folate concentration is positively associated with serum total cholesterol and low-density lipoprotein across the three trimesters of pregnancy
Authors: da Silva Manoela T. , Mujica-Coopman Maria F. , Figueiredo Amanda C. C. , Hampel Daniela , Vieira Luna S. , Farias Dayana R. , Shahab-Ferdows Setareh , Allen Lindsay H. , Brito Alex , Lamers Yvonne , Kac Gilberto , S. Vaz Juliana 
Published: November 2020

AbstractIncreased first-trimester low-density lipoprotein (LDL-C) concentration has been associated with adverse pregnancy outcomes, such as gestational diabetes. The B vitamins folate, B-6, and total B-12 are key for the methyl group-dependent endogenous synthesis of phosphatidylcholine, which is needed for lipoprotein synthesis, e.g., very low-density lipoprotein (VLDL), the precursor of circulating LDL-C. Maternal B-vitamin concentration usually declines across trimesters. Whether changes in maternal B-vitamin concentrations are associated with total cholesterol (TC), triglycerides (TG), and lipoprotein concentrations is unknown. Therefore, we explored the association between plasma folate, vitamin B-6 in the form of pyridoxal 5′-phosphate (PLP), and total B-12 with serum TC, LDL-C, HDL-C, and TG concentrations across trimesters. This secondary analysis used data of a prospective pregnancy cohort study included apparently healthy adult women (n = 179) from Rio de Janeiro, Brazil. The biomarkers were measured in fasting blood samples collected at 5–13, 20–26, and 30–36 weeks of gestation. The associations between B vitamins and lipid concentrations across trimesters were explored using linear mixed-effect models. Among B vitamins, only plasma folate was positively associated with TC (β = 0.244, 95% CI 0.034–0.454) and LDL-C (β = 0.193, 95% CI 0.028–0.357) concentrations. The positive relationship of maternal folate and TC and LDL-C concentrations may indicate the importance of folate as a methyl donor for lipoprotein synthesis during pregnancy.
In vivo oscillations of the soleus muscle can be quantified using b-mode ultrasound imaging during walking and running in humans
Authors: Lai A. K. M. , Hodson-Tole E. F. 
Published: November 2020

AbstractImpact forces, due to the foot contacting the ground during locomotion, can be considered input signals to the body that must be dissipated to prevent impact-related injuries. One proposed mechanism employed by the body to damp the impact is through vibrations of the skeletal muscles. However, there is yet to be direct in vivo measures of muscle oscillations during locomotion. This study investigated the use of 2D ultrasound imaging to quantify transverse muscle oscillations (deep-superficial displacement of the muscle boundary relative to the skin) in response to impact forces elicited by walking and running at a range of speeds. Increases in vertical impact forces with faster walking and running was consistent with changes in both magnitude and frequency in the measured oscillations of the soleus muscle; one of the main human ankle plantar flexors. Muscle oscillations contained more higher frequency components at fast running (50% signal power in frequencies below ~ 14 Hz) compared with slow walking (50% signal power contained in frequencies below ~ 5 Hz). This study provides a platform for ultrasound imaging to examine muscle oscillation responses to impact forces induced by changes in external interfaces such as shoe material, locomotion type and ground surface properties.
Formation of the hollow nanopillar arrays through the laser-induced transformation of TiO2 nanotubes
Authors: Wawrzyniak Jakub , Karczewski Jakub , Kupracz Piotr , Grochowska Katarzyna , Coy Emerson , Mazikowski Adam , Ryl Jacek , Siuzdak Katarzyna 
Published: November 2020

AbstractIn the following article, we present a simple, two-step method of creating spaced, hollow nanopillars, from the titania nanotube arrays via pulsed laser-treatment. Due to the high ordering of the structure, the prepared material exhibits photonic properties, which has been shown to increase the overall photoefficiency. The optical and morphological changes in the titania nanotubes after pulsed laser-treatment with 532, 355, and 266 nm wavelengths in the 10–50 mJ/cm2 fluence range are studied. The investigation reveals, that by using appropriate wavelength and energy, the number of surface defects, geometrical features, or both can be tailored.
In vitro dexamethasone treatment does not induce alternative ATM transcripts in cells from Ataxia–Telangiectasia patients
Authors: Pozzi Elisa , Giorgio Elisa , Mancini Cecilia , Lo Buono Nicola , Augeri Stefania , Ferrero Marta , Di Gregorio Eleonora , Riberi Evelise , Vinciguerra Maria , Nanetti Lorenzo , Bianchi Federico Tommaso , Sassi Maria Paola , Costanzo Vincenzo , Mariotti Caterina , Funaro Ada , Cavalieri Simona , Brusco Alfredo 
Published: November 2020

AbstractShort term treatment with low doses of glucocorticoid analogues has been shown to ameliorate neurological symptoms in Ataxia–Telangiectasia (A–T), a rare autosomal recessive multisystem disease that mainly affects the cerebellum, immune system, and lungs. Molecular mechanisms underlying this clinical observation are unclear. We aimed at evaluating the effect of dexamethasone on the induction of alternative ATM transcripts (ATMdexa1). We showed that dexamethasone cannot induce an alternative ATM transcript in control and A–T lymphoblasts and primary fibroblasts, or in an ATM-knockout HeLa cell line. We also demonstrated that some of the reported readouts associated with ATMdexa1 are due to cellular artifacts and the direct induction of γH2AX by dexamethasone via DNA-PK. Finally, we suggest caution in interpreting dexamethasone effects in vitro for the results to be translated into a rational use of the drug in A–T patients.
Sparse LMS algorithm for two‐level DSTATCOM
Authors: Mangaraj Mrutyunjaya , Panda Anup Kumar 
Published: November 2020

AbstractSparse least mean square algorithm is proposed for the DSTATCOM as an optimal current harmonic extractor to cope with the intermittent nature of loadings. Sparse least mean square is the improved version of adaptive least mean square learning mechanism with regards to sparsity. This innovative approach is utilized for better parameter estimation due to its algorithmic simplicity and parallel computing nature. Hence, sparse least mean square is expected to reduce the computation and storage requirements significantly. This suggested controller consists of six subnets. Three subnets for active and another three for reactive weight component are used to extract the fundamental component of the load current. Several factors like previous weight, normalizing weight and learning rate are involved in the sparse least mean square based weight‐updating rule to have better dynamic performance, reduced computational burden and better estimation speed etc. The detailed control algorithm is formulated using MATLAB/Simulink, and validated using experimental analysis. Among these two algorithms, the sparse least mean square offers better voltage regulation, voltage balancing, source current harmonic reduction and power factor correction under various loading scenarios.
VLA ammonia observations of L1287
Authors: Sepúlveda Inma , Estalella Robert , Anglada Guillem , López Rosario , Riera Angels , Busquet Gemma , Palau Aina , Torrelles José M. , Rodríguez Luis F. 
Published: November 2020

Aims. In this paper, we study the dense gas of the molecular cloud LDN 1287 (L1287), which harbors a double FU Ori system, an energetic molecular outflow, and a still-forming cluster of deeply embedded low-mass young stellar objects that show a high level of fragmentation.
Methods. We present optical Hα and [SII], and VLA NH3 (1, 1) and (2, 2) observations with an angular resolution of ~3′′.5. The observed NH3 spectra have been analyzed with the Hyperfine Structure tool, fitting simultaneously three different velocity components.
Results. The NH3 emission from L1287 comes from four different structures: a core associated with RNO 1, a guitar-shaped core (the Guitar) and two interlaced filaments (the blue and red filaments) roughly centered toward the binary FU Ori system RNO 1B/1C and its associated cluster. Regarding the Guitar core, there are clear signatures of gas infall onto a central mass that has been estimated to be ~2.1M⊙. Regarding the two filaments, they have radii of ~0.03 pc, masses per unit length of ~50M⊙ pc−1, and are in near isothermal equilibrium. A central cavity is identified, probably related with the outflow and also revealed by the Hα and [SII] emission, with several young stellar objects near its inner walls. Both filaments show clear signs of perturbation by the high-velocity gas of the outflows driven by one or several young stellar objects of the cluster. The blue and red filaments are coherent in velocity and have nearly subsonic gas motions, except at the position of the embedded sources. Velocity gradients across the blue filament can be interpreted either as infalling material onto the filament or rotation. Velocity gradients along the filaments are interpreted as infall motions toward a gravitational well at the intersection of the two filaments.
mGreenLantern: a bright monomeric fluorescent protein with rapid expression and cell filling properties for neuronal imaging
Authors: Campbell Benjamin C. , Nabel Elisa M. , Murdock Mitchell H. , Lao-Peregrin Cristina , Tsoulfas Pantelis , Blackmore Murray G. , Lee Francis S. , Liston Conor , Morishita Hirofumi , Petsko Gregory A. 
Published: November 2020

Significance
We have developed a fluorescent protein, mGreenLantern, that features exceptionally high brightness in mouse, bacterial, and human cells (up to sixfold brighter than EGFP) and have demonstrated its superior ability to highlight neuronal morphology compared to EGFP and EYFP. Screening fluorescent protein mutants based on whole-cell brightness while evaluating expression kinetics in lysate enabled us to identify variants exhibiting striking divergences between their computed spectroscopic brightness and actual performance in cells. mGreenLantern additionally features unusually high chemical and thermodynamic stability and is compatible with existing GFP filter sets, excitation sources, commercial EGFP antibodies, expansion microscopy, and whole-brain tissue clearing. Our hypothesis-driven engineering strategy represents a generalizable method with great potential to enhance the performance of constitutive reporters and GFP-based biosensors.
Current methods for GaN synthesis and the limitations
Authors: Wang Yuguo 
Published: November 2020

Abstract
The application of GaN has greatly enhanced the performance of optoelectrical and microelectronic devices. However, the industrial application still faces huge challenges due to the difficulty during the synthesis process. This paper introduces several current methods for GaN synthesis and talks about their advantages and limitations. Four methods are introduced: high pressure nitrogen solution growth process, ammonothermal method, sodium flux process and hydride vapor phase epitaxy. As a result, they are only suitable for specific use like scientific research and can’t be used for massive production. This paper reviews main GaN synthesis methods and points out the limitations they have to overcome for commercial applications.
Antioxidant Activity of the Extracts from Tussilago farfara
Authors: Wei Xiang , Shang Huilan , Yang Aimei , Wang Zhihui 
Published: November 2020

Abstract
Objective: The antioxidative properties of Chinese medicine Tussilago farfara were determined by spectrophotometry. Methods: The anti-oxidation activity of petroleum ether, ethyl acetate and n-butanol extracts from ethanol extract of Tussilago farfar were studied, using DPPH free radical scavenging, hydroxyl radical scavenging, and total reducing power determination. Results: Compared with the Vc activity of the positive control, the extracts of ethyl acetate part and the n-butanol part showed significant antioxidant activity, and the antioxidant activity in the ethyl acetate part was higher than Vc. Conclusion: Antioxidant activity of ethyl acetate extract is better than vitamin C, N-butanol extract also has obvious antioxidant activities.
Analysis on Research Status of Loess Microstructure
Authors: Xie Xiao , Wei Yulu 
Published: November 2020

Abstract
Chinese loess has a wide range of distribution, large strata thickness, and high stratigraphic completeness, and has been widely studied by scholars. By consulting and summarizing literatures, this paper generalizes the previous research results, and summarizes the microstructure characteristics of the loess, such as loess skeleton particles, the way they are connected to each other, the pore types, and the regional changes of the loess microstructure. Finally, the prospects of relevant researches on the loess microstructure were prospected.
Effect of Glaze Composition on the Impact Resistance of Glass Lining of Glass-Lined Vessels
Authors: Xu Jinsha , Ren Facai , Liu Chongyang , Tang Chenhuai , Han Shoupeng , Chen Yi , Song Youli , Lin Jinfeng 
Published: November 2020

Abstract
Glass-lined pressure vessels are widely used in various corrosion conditions because of their excellent corrosion resistance. However, mechanical impact is the most common failure mode of glass linings due to its brittleness. Under the same firing process condition, the impact-resistant properties of glass-lined vessels are largely dependent upon the glaze composition. In this paper, three kinds of glazes were selected to study the impact resistance of the glass lining. Based on the glaze composition analysis, the impact energy measurement and the micromorphology observations of the impact surfaces, the effect of glaze composition on the impact resistance of glass lining was discussed. The results showed that the low content of refractory oxides such as SiO2, ZrO2 and Al2O3 made the glaze easy to melt and with high-temperature fluidity. Therefore, the structure of the glass lining was dense, and glass lining exhibited good resistance to impact.
A study on the Friction Stir Welded Non-ferrous Alloy
Authors: Xu Anlian 
Published: November 2020

AbstractFriction stir welding (FSW), which is a new-type solid state welding technology, has been successfully used in aluminum alloys, magnesium alloys and coppers. To study the properties of friction stir welded zinc-based non-ferrous alloys, Zn-0.5Cu alloy plates were butt welded by FSW. The results show that Zn-0.5Cu alloy plates with 5 thickness can be successfully welded by FSW. The joint efficiency of weld joint can reach over 95 % at appropriate weld process parameters.
Research on Large-diameter Solid Expandable Tubular Subsidy Technology and Suspension Seal Device
Authors: Fu Daqi , Zhu Xiaohua , Qi Yuekui , Shao Lifei , Shi Changshuai , Chen Kailin , Huang Manlinag , Zhao Tao 
Published: November 2020

Abstract
In view of the issue that the diameter is small and the suspension force is uncertain after solid expandable tubular (SET) subsidy. The uniaxial tensile test of SA106B metal is carried out, and the parameters of metal elastoplastic constitutive model are fitted accordingly. Then, a solid expandable tubular laboratory test is designed, and a finite element model of SET is established based on this test. Finally, the effects of the expansion cone radius and the thickness of the rubber ring on the expansion stress and contact pressure are researched. The results show that the established finite element model has small errors and reliable results. The optimal expansion cone radius is selected through the maximum stress and the residual stress of expanded tubular to improve the expansion quality of the tube. The thickness of the rubber ring has a great influence on the contact pressure and the suspension force, so it must be reasonably optimized under the premise of ensuring the sealing ability. The optimal solution of the expansion cone radius and the thickness of the rubber ring is given by comparison. This study provides a theoretical basis for the optimization of solid expandable tubular technology.