bluuebunny/crossref_metadata_2025_split huggingface dataset:
HPV 18 Variants in Women with Cervical Cancer in Northeast Brasil
Authors: Santos Gerusinete Rodrigues Bastos dos , Cunha Ana Paula Almeida , Batista Zulmira da Silva , Silva Rodrigo Lopes da , Costa Lucas Henrique de Lima , Castro Lailson Oliveira de , Silva Marcos Antonio Custódio Neto da , Figueiredo Fábio Vidal de , Santos Flávia Rodrigues Bastos dos , Moreira Miguel Ângelo Martins , Fraga Elmary da Costa , Barros Maria Claudene , Vidal Flávia Castello Branco , Brito Luciane Maria Oliveira , Nascimento Maria do Desterro Soares Brandão 
Published: July 2020

Abstract
Background: Human Papillomavirus (HPV) is the main etiological factor for the development of cervical cancer. HPV 18 is the second most frequent, accounting for up to 65% of all cases. HPV intratypic variation may influence the potential for progression to invasive cancer.The aim of this study was to evaluate the prevalence of human papillomavirus 18 intratypic variants in cervical cancer samples from women in the Northeast of Maranhão. Methods: The study was composed of 118 women over 18 years of age with a diagnosis of cervical cancer. Tumor fragments were collected and subjected to DNA extraction and Polymerase Chain Reaction (PCR) for HPV detection using the PGMY09/11 and GP+5/6 primers. Positive samples were submitted to automated sequencing for viral genotyping. To determine the HPV 18 lineages, positive samples were submitted to PCR, using specific primers to amplify the LCR and E6 regions of HPV 18 virus. Results: HPV was present in 88 women (73.3%), and HPV 16 was the most prevalent (48/54%), followed by HPV 18 (12/13.6%). Histologically, squamous cell carcinoma was predominant (79.1%). Among the HPV 18 variants identified, 10 (80%) belonged to lineage A, and sublineages A1, A2, A3, and A4. HPV 18 B variant (2/29%) was also detected, with the sublineages B1 and B2. In this study, the C variant was not found. There was no statistically significant association between the HPV 18 lineages found and sociodemographic and lifestyle variables (p > 0.05). Conclusions: Women with cervical cancer are associated with a higher frequency of HPV 16 and 18 in the Northeast region of Brazil, with a high prevalence of the lineage A among women with HPV 18.
Effects of Prenatal Mindfulness-Based Childbirth Education on Child-Bearers’ Trajectories of Distress: A Randomized Control Trial
Authors: Sbrilli Marissa Danielle , Duncan Larissa G. , Bardacke Nancy , Laurent Heidemarie K. 
Published: July 2020

Abstract
The perinatal period is a time of immense change, which can be a period of stress and vulnerability for mental health difficulties. Mindfulness-based interventions have shown promise for reducing distress, but further research is needed to identify long-term effects and moderators of mindfulness training in the perinatal period. The current study used data from a pilot randomized control trial (RCT) comparing a condensed mindfulness-based childbirth preparation program—the Mind in Labor (MIL)—to treatment as usual (TAU) to examine whether prenatal mindfulness training results in lower distress across the perinatal period, and whether the degree of benefit depends on child-bearers’ initial levels of risk (i.e., depression and anxiety symptoms) and protective (i.e., mindfulness) characteristics. Child-bearers (N=30) in their third trimester were randomized to MIL or TAU and completed assessments of distress—perceived stress, anxiety, and depressive symptoms—at pre-intervention, post-intervention, six-weeks post-birth, and one-year postpartum. Multilevel modeling of distress trajectories revealed greater decreases from pre-intervention to 12 months postpartum for those in MIL compared to TAU, especially among child-bearers who were higher in anxiety and/or lower in dispositional mindfulness at baseline. The current study offers preliminary evidence for durable perinatal mental health benefits following a brief mindfulness-based program and suggests further investigation of these effects in larger samples is warranted.Trial registration: The ClinicalTrials.gov identifier for the study is: NCT02327559. The study was retrospectively registered on June 23, 2014. https://clinicaltrials.gov/ct2/show/NCT02327559
Characteristics and Treatments for Pediatric Ordinary and Incarcerated Inguinal Hernia Based on Gender: 12-Year Experiences from a Single Center
Authors: Wang Kai , Tan Sarah Siyin , Xiao Yue , Wang Zengmeng , Peng Chunhui , Pang Wenbo , Wu Dongyang , Chen Yajun 
Published: July 2020

Abstract
Background: To describe the different characteristics of pediatric inguinal hernia in a large population for both genders based on our 12-year experience, and to accordingly recommend one of the two commonly used methods of hernia repair: the traditional open inguinal hernia repair (OIHR) and laparoscopic inguinal hernia repair (LIHR). An additional goal was to describe the characteristics of incarcerated inguinal hernia.Methods: Patients with unilateral or bilateral inguinal hernia who were admitted to our hospital and received OIHR or LIHR under general anesthesia were included. Medical records were retrospectively collected and analyzed.Results: A total of 12190 patients were included in this study. The ratio of male to female was 4.8:1. There was a total of 10646 unilateral hernias (87.3%) and 1544 bilateral hernias (12.7%), with a corresponding ratio of 6.9:1. 12444 hernia repair surgeries, 11083 (89.1%) OIHR and 1361 (10.9%) LIHR, were held. OIHR had a shorter operative time than LIHR for all unilateral and female bilateral repair, unlike for bilateral male repair. There was no difference between OIHR and LIHR for ipsilateral recurrent hernia in males. There was a difference between OIHR and LIHR for metachronous contralateral hernia.Incarcerated inguinal hernia was associated with longer operative time, hospital stay and higher hospital costs. Males and patients under 1 year were more likely to present with incarcerated hernia.Conclusions: OIHR should be considered for male patients, especially for unilateral and complete inguinal hernia. LIHR is highly recommended for female patients. For incarcerated hernia, attention should be paid to patients under 1 year old, as they can be 60 times more susceptible, and males. Surgeons should also be aware of ovary hernias in females.
Global, regional, and national burdens of bladder cancer in 2017: estimates from the 2017 Global Burden of Disease study
Authors: He Hairong , Xie Hongjun , Chen Yule , Li Chengzhuo , Han Didi , Xu Fengshuo , Lyu Jun 
Published: July 2020

Abstract
Background
The aim of this study is to describe the prevalence and mortality rates of bladder cancer (BCa) using data obtained in the Global Burden of Disease study performed in 2017 (GBD 2017).
Methods
Data on BCa for 2017, including prevalence, mortality, and disability-adjusted life years (DALYs), were obtained from GBD 2017 at the global, regional, and national levels. We also analyzed the association of BCa burden with the country development level.
Results
There were 2.63 million BCa cases estimated from the GBD 2017 data, with 200,000 persons dying of BCa, resulting in 3.60 million DALYs in 2017. The age-standardized prevalence rate (ASPR) of BCa was 32.91/100,000 persons, and age-standardized death rate (ASDR) was 2.57/100,000 persons. The ASPR and ASDR of BCa were higher in males than in females, and higher in people older than 60 years. The ASPR and ASDR of BCa were higher in Western Europe and Central Europe than in South Asia, Andean Latin America, and Central Latin America, and higher in countries with a higher sociodemographic index (SDI). Correlation analysis identified that the ASPR and ASDR of BCa were positively correlated with the country SDI (P < 0.0001 and ρ = 0.68 for ASPR, and P = 0.0048 and ρ = 0.20 for ASDR). Smoking was the main risk factors attributable to the BCa burden.
Conclusion
The prevalence and mortality rates of BCa were very high in 2017, especially in high-SDI countries. Smoking-cessation strategies should be strengthened to control the burden associated with BCa.
Southern Ocean in-situ temperature trends over 25 years emerge from interannual variability
Authors: Auger Matthis , Morrow Rosemary , Kestenare Elodie , Sallée Jean-Baptiste 
Published: July 2020

Abstract
Despite playing a major role for the global ocean heat storage, the Southern Ocean remains the most sparsely measured region of the global ocean. Here, a unique 25-year temperature time-series of the upper 800 m, repeated several times a year across the Southern Ocean, allows us to document the long-term change within water-masses and how it compares to the interannual variability. Three regions stand out as having strong change that is radically different from the interannual variability: warming of the subantarctic waters (0.29±0.09°C per decade); cooling of the near-surface subpolar waters (-0.07±0.04°C per decade); and warming of the subsurface subpolar deep waters (0.04±0.01°C per decade). Our results highlight that this subsurface warming of subpolar deep waters is, counter-intuitively, the largest change of the section regarding interannual variability. This robust warming is associated with a large shallowing (39±11 m per decade), which has been significantly underestimated by a factor of 3 to 10 in past studies. We find temperature changes of comparable magnitude to those reported in West Antarctica, which calls for a reconsideration of current ocean changes with important consequences for our understanding of future Antarctic ice-sheet mass loss.
A genome-wide association study of colorectal cancer in Mexican mestizos suggest novel common tumor-risk variants
Authors: Rojas-Martinez AUGUSTO , Colistro Valentina , Cruz Raquel , Ruiz Clara , Quintela Inés , Carracedo Angel , Luna-Perez Pedro , Garcia-Gonzalez Irma S. , Martinez-Murillo Carlos , Ortiz-Lopez Rocio , Jaramillo-Rodriguez Yolanda , Ruiz-Flores Pablo , Castelan-Maldonado Edmundo , Gonzalez-Guerrero Juan F. , Cardenas-Cadena Sergio , Moncada-Saucedo Nidia K. , cruz Jorge Haro-Santa , Alvarado-Monroy Fatima M. , Sans Monica , Martínez Augusto Rojas 
Published: July 2020

Abstract
Background: Genome-wide association studies (GWAS) for colorectal cancer (CRC) have detected high-risk genetic variants associated with CRC in several ethnic groups, but Latin American communities are still underrepresented. The aim was to identify variants related to CRC in an admixed Latin American population. Methods: The study was performed in 831 cases and 881 controls from Mexico, who were genotyped for 1,006,703 autosomal SNPs. Logistic regression was carried out including covariants, such as sex, age and genetic ancestry. Lastly, we performed a sequence-kernel association test (SKAT) to consider the joint effect of several SNPs lying in genes.Results: Eight chromosomal regions reached genome-wide significance level ( p < 5×10 -8 ): 1p36.22, 1p31.1, 1q42.13, 6p22, 7p14.1, 12q24.32, 16q12.2 and 21q22.2 and 63 variants reached borderline statistical significance ( p < 1×10 − 6 ). SKAT analysis detected 13 loci associated with CRC, none of them previously associated with CRC. Conclusions: We found 8 SNPs and 13 loci associated with CRC. These signals may contribute to enrich the panoply of genes involved with CRC. Further analyses remain to be done to validate the associations in other Latin American populations. This study highlights the importance of conducting GWAS in poorly explored admixed populations.
Process and Resource Assessment in Trials Undertaken in Residential Care Homes: Experiences from the Australian MIDDEL Cluster Randomised Controlled Trial Research Team.
Authors: Baker Felicity Anne , Stretton-Smith Phoebe , Sousa Tanara Vieira , Clark Imogen , Cotton Alice , Gold Christian , Lee Young-Eun Claire 
Published: July 2020

Abstract
Background: The resources involved in delivering a clinical trial in residential aged care facilities (RACFs) are significant and the success of a trial is dependent upon adequate planning, including appropriate timelines for each component of the study and the required budget. The main aim of this paper is to describe process and resource assessment during recruitment, collection of outcome measures and intervention delivery and present learnings and considerations for conducting trials in RACFs with people living with dementia. Methods: We collected data across 24 clusters in 12 RACFs over 18 months during a cluster randomised controlled trial which was testing the effectiveness of music interventions in people living with dementia. Data were collected on resources required for recruitment and assessment of baseline data, as well as data on reasons for participant non-attendance at the interventions. Results: Results show that time between contacting next of kin and receiving formal consent often exceeded 45 days and the ratio of time between direct and indirect research activity is approximately 1:2. Participant intervention adherence is at risk from unplanned RACF lockdowns and reasons for non-attendance include those both related directly to the participant and to staff resources, scheduling or other practical considerations. Conclusions: Researchers planning studies within RACFs should focus on building relationships with RACF staff and resident families, factor in adequate time for recruitment in the study timeline and consider budgeting for backfill of RACF staff during data collection phases to expedite the process and ensure adherence to study protocol timelines. This study provides specific data on resource assessment and intervention adherence that could be beneficial for future researchers planning to conduct trials in RACFs with people with dementia. Trial registration: Australian and New Zealand Clinical Trial Registry: ANZCTR12618000156280, 1/02/2018, http://anzctr.org.au/Trial/Registration/TrialReview.aspx?ACTRN=12618000156280
Continental-Scale Paddy Soil Bacterial Community Structure, Function and Biotic Interaction
Authors: Zhang Zhijian , Li Hong-Yi , Wang Hang , Tao Xing-Hua , Wang Xian-Zhe , Jin Wei-Zheng , Chen Hong , Gilbert Jack , Zhu Yong-Guan 
Published: July 2020

Abstract
Background: Rice paddy soil-associated microbiota participate in biogeochemical processes that underpin rice yield and soil sustainability, yet continental-scale biogeographic patterns of paddy soil microbiota remain elusive. Here, the soil bacteria of four typical Chinese rice-growing regions were characterized over large-scale space and compared with adjacent non-paddy soils.Results: The geographic patterns of paddy soil bacteria were significantly different from non-paddy soils, with lower alpha diversity, unique taxonomic and functional composition, and distinct co-occurrence network topology. Both stochastic and deterministic processes shaped soil bacteria assembly, but paddy exhibited a stronger deterministic signature than non-paddy samples, especially due to the roles of climate determinants. The continental biogeographic variance in bacterial community structure was driven by the competition between two mutually-exclusive bacterial modules in the co-occurrence network, and suggested antagonistic species-to-species interactions as potential selective forces may greatly shape their community structures. Keystone taxa identified in network models, such as Actinobacteria, Chloroflexi, and Proteobacteria, were demonstrated to be preferentially affected by environmental factors than other community members and showed high sensitivity to environmental changes, whereby the environmental factors greatly shaped the paddy soil bacterial communities by leveraging changes in keystones.Conclusions: The strong interplay between biotic/abiotic factors may greatly construct paddy soil microbial community and their uniqueness as compared with non-paddy soils. Microbial biogeographical analyses with novel insights into underlying determinants investigated on intensively-cultivated paddy field soils may aid in elucidating microbial changes subjected to land-use changes following the transformation between natural and agro-ecosystem, and also facilitate microbial community manipulation for better crop productivity and soil sustainability worldwide.
Functional and Radiological Outcomes After Treatment with Custom-Made Acetabular Components in Patients with Paprosky type 3 Acetabular Defects: Short-Term Results
Authors: Gruber Michael Stephan , Jesenko Michael , Burghuber Julia , Hochreiter Josef , Ritschl Peter , Ortmaier Reinhold 
Published: July 2020

Abstract
BackgroundSevere acetabular defects require special treatment with either impaction bone grafting, metal augmented cups or cup-cage constructs. Even these options are often not adequate, especially in hips with Paprosky type 3 defects with loss of anterior and posterior columns. This study investigates the clinical and radiological outcomes of custom-made acetabular components (© Materialise NV, Leuven, Belgium) for Paprosky type 3 defects.MethodsSixteen patients were included in this trial, nine of whom completed one year of follow-up. The Harris hip score and the Oxford hip score were used to compare pre- and postoperative functional outcomes. Radiological follow-up comprised anteversion and inclination of the implanted cup and offset measurements in both hips (femoral, medial, ischial offset and center of rotation). Statistical analyses were performed with IBM SPSS Statistics.ResultsThe mean follow-up time of the nine patients was 13.6 months (± 5.6 SD). The Oxford hip score and Harris hip score improved from 19.8 and 50.1 to 29.4 and 68.8, respectively (p = 0.009 and 0.01). There were complications in three cases (33.3%), which led to one re-revision (11.1%). Radiologic follow-up showed no significant difference in offset measurements except for femoral offset (41.8 mm on the contralateral and 35.2 mm on the treated hip, p = 0.044).ConclusionsThe functional and radiological outcomes are promising. However, long-term outcomes still need to be examined.Level of Evidence: Therapeutic Level IV.
Effect of Real-Time Ultrasound Imaging for Biofeedback on Trunk Muscle Contraction in Healthy Subjects: A Preliminary Study
Authors: Lin Shanshan , Zhu Bo , Zheng Yiyi , Huang Guozhi , Zeng Qing , Wang Chuhuai 
Published: July 2020

Abstract
Background: Real-time ultrasound imaging (RUSI) has been increasingly used in clinical medicine as a form of biofeedback when instructing and re-training muscle contraction. However, the effectiveness of the RUSI on a single sustained contraction of the lumbar multifidus (LM) and transversus abdominis (TrA) has rarely been reported. This preliminary study aimed to determine if the use of RUSI as visual biofeedback enhances the ability of activation and continuous contraction of the trunk muscles including LM and TrA.Methods: Forty healthy individuals were included and assigned to 2 groups. All subjects received clinical instruction in how to perform a preferential activation of the LM and TrA, while subjects in the experimental group also received visual feedback (watched the LM and TrA contraction) provided by RUSI. All subjects maintained the LM and TrA contraction for 30 seconds, and the thickness of LM and TrA at rest and during contraction (Tc-max, T15s, and T30s) were extracted and recorded.Results: Non-significant differences were found in the thickness of LM at rest (P > 0.999), Tc-max (P > 0.999), and T15s (P = 0.414) between groups. However, the ability to recruit LM muscle contraction differed between groups at T30s (P = 0.006), with subjects in the experimental group that received visual ultrasound biofeedback maintaining a relative maximum contraction. Besides, there were also no significant differences in the TrA muscle thickness at rest (P > 0.999) and Tc-max (P > 0.999) between groups. While, significant differences of contraction thickness were found at T15s (P = 0.031) and T30s (P = 0.010) between groups during the ADIM, with greater TrA muscle contraction thickness in the experimental group.Conclusions: RUSI can be used to provide visual biofeedback, which can promote continuous contraction efficiency, improve performance, and retention in the ability to activate the LM and TrA muscles in healthy subjects.
Identification of the Window Period Concurrent Infection with Syphilis and HIV: A Longitudinal Case Study
Authors: Wang Lilin , Shao Chaopeng , Hong Wenxu , Zhu Weigang , Lu Liang , Yang Zhengrong , Zhao Fang , Liu Heng , Zheng Xin , Wang Lunan , Zeng Jinfeng 
Published: July 2020

Abstract
BACKGROUND: Due to the low incidence of concurrent human immunodeficiency virus (HIV) and syphilis infection identified during the window period, little is known about the clinical manifestations, diagnosis, and treatment efficacy at very early stages.METHODS: One longitudinal study was conducted in a 42-year-old blood donor who was concurrently infected with syphilis and HIV. This blood donor was treated with a penicillin-based regimen and early antiretroviral therapy (ART). Immunological and NAT methods were comparatively analyzed.RESULTS: A regular male donor who had two occasions of high-risk sexual behaviors 41 and 35 days before donation(DBD) donated whole blood at the Shenzhen Blood Center. Antiretroviral therapy was initiated at the 28th day after donation (DAD), and syphilis treatment was received at the 49th DAD. Microbiological analysis using a 4th generation anti-HIV ELISA and CLIA indicated a positive signal at the 6th DAD. All NAT tests for HIV RNA were reactive except the minipool (MP) NAT of six pooled samples at 117th DAD. The HIV viral load declined more than 4-log in copies per milliliter over 3 months, until reaching non-detectable levels at 246th DAD. Nevertheless, HIV-1 DNA was still detectable at 403rd DAD. Among all methods utilized, anti-TP ELISA detected syphilis infection at the earliest time. Toluidine red unheated serum test (TRUST) was negative around the 80th DAD.CONCLUSION: Concurrent infection with Syphilis and HIV during window period did not significantly change the sensitivity of reagents in detection nor alter the therapeutic efficacy for the treatment of both pathogens.
Embedded Internet of Things Applications of SQLite Based on WinCE Mobile Terminal
Authors: Yu Yuanhui 
Published: July 2020

Abstract
At present, embedded applications on the WinCE platform are extending towards microservices and miniaturization. More embedded device application data requires small, embedded database systems to organize, store, and manage. The embedded database SQLite has many advantages such as zero-configuration, lightweight, multiple interfaces, easy portability, readable source code, and open source. It is currently widely used in the WinCE embedded operating system. This article discusses the technical characteristics of the SQLite database in detail, SQLite data manipulation, SQLite transplantation under the WinCE platform, and finally implements SQLite data management on WinCE mobile terminal based on MFC programming.