bluuebunny/crossref_metadata_2025_split huggingface dataset:
Analysis of the Temporal Patterning of Notch Downstream Targets during Drosophila melanogaster Egg Chamber Development
Authors: Rowe Molly , Paculis Lily , Tapia Fernando , Xu Qiuping , Xie Qian , Liu Manyun , Jevitt Allison , Jia Dongyu 
Published: April 2020

AbstractLiving organisms require complex signaling interactions and proper regulation of these interactions to influence biological processes. Of these complex networks, one of the most distinguished is the Notch pathway. Dysregulation of this pathway often results in defects during organismal development and can be a causative mechanism for initiation and progression of cancer. Despite previous research entailing the importance of this signaling pathway and the organismal processes that it is involved in, less is known concerning the major Notch downstream targets, especially the onset and sequence in which they are modulated during normal development. As timing of regulation may be linked to many biological processes, we investigated and established a model of temporal patterning of major Notch downstream targets including broad, cut, and hindsight during Drosophila melanogaster egg chamber development. We confirmed the sequential order of Broad upregulation, Hindsight upregulation, and Cut downregulation. In addition, we showed that Notch signaling could be activated at stage 4, one stage earlier than the stage 5, a previously long-held belief. However, our further mitotic marker analysis re-stated that mitotic cycle continues until stage 5. Through our study, we once again validated the effectiveness and reliability of our MATLAB toolbox designed to systematically identify egg chamber stages based on area size, ratio, and additional morphological characteristics.
Viviparous stingrays avoid contamination of the embryonic environment through faecal accumulation mechanisms
Authors: Tomita Taketeru , Nakamura Masaru , Kobayashi Yasuhisa , Yoshinaka Atsushi , Murakumo Kiyomi 
Published: April 2020

AbstractIn viviparous (live-bearing) animals, embryos face an embryo-specific defecation issue: faecal elimination in utero can cause fatal contamination of the embryonic environment. Our data from the viviparous red stingray (Hemitrygon akajei) reveals how viviparous elasmobranchs circumvent this issue. The exit of the embryonic intestine is maintained closed until close to birth, which allows the accumulation of faeces in the embryonic body. Faecal accumulation abilities are increased by (1) the large intestine size (represents about 400–600% of an adult intestine, proportionally), and (2) the modification in the intestinal inner wall structure, specialized to increase water uptake from the faecal matter. According to the literature, faecal accumulation may occur in embryos of the lamniform white shark as well. The reproductive biology of myliobatiform stingrays and lamniform sharks is characterized by the onset of oral feeding before birth (i.e. drinking of uterine milk and eating of sibling eggs, respectively), which is expected to result in the production of large amounts of faeces during gestation. The strong ability of faecal accumulation in these lineages is therefore likely an adaptation to their unique embryonic nutrition mechanism.
Different histopathologic profiles and outcomes between sun-exposed BCC and non-sun-exposed BCC
Authors: Li Chia-Lun , Chen Yu-Ching , Yang Kuo-Chung , Chen Lee-Wei 
Published: April 2020

AbstractAsian population is a low-risk group for basal cell carcinoma (BCC) and there is little data available in this setting. Sun-exposed BCC (SEBCC) may possess a different pathogenic mechanism from non-sun-exposed BCC (NSEBCC). To compare the histopathological profiles and outcomes between SEBCC and NSEBCC, and to assess the risk factors for tumor recurrences. Retrospective cohort study on 372 patients with pathologically diagnosed BCC from January 1, 1990 to August 31, 2017. Data were derived from a single medical center in Taiwan. SEBCC presented with higher Clark level and more high-risk factors for recurrence than NSEBCC. Nodular, micronodular, infiltrating/mixed infiltrating, basosquamous, and adenoid types were predominant in SEBCC, as superficial type in NSEBCC. Risk factors for recurrence included infiltrating/mixed-infiltrating subtypes and synchronous basosquamous cell carcinoma. No recurrence events were observed in NSEBCC. Our study showed an acceptable recurrence rate (4.2%) of the whole population after excision even under a smaller surgical margin width than suggested by current guidelines. SEBCC had a higher recurrence rate with a significantly different tumor characteristic from NSEBCC and a greater tumor depth than NSEBCC. A wider surgical margin in SEBCC than NSEBCC is suggested.
Protein-protein interaction of the putative magnetoreceptor cryptochrome 4 expressed in the avian retina
Authors: Wu Haijia , Scholten Alexander , Einwich Angelika , Mouritsen Henrik , Koch Karl-Wilhelm 
Published: April 2020

AbstractMigratory birds can sense the Earth’s magnetic field and use it for orientation over thousands of kilometres. A light-dependent radical-pair mechanism associated with the visual system is currently discussed as the underlying mechanism of the magnetic compass sense. The blue light receptor cryptochrome 4 (Cry4) is considered as the most likely primary sensory protein that detects the geomagnetic field. Since the protein interaction partners of Cry4 are completely unknown at present, here, we aim to identify potential candidate interaction partners of Cry4 in the avian retina. We used the yeast-two-hybrid system to screen avian cDNA libraries for possible interaction partners of Cry4 in the European robin. The UAS-GAL yeast two hybrid system was applied to confirm a group of candidate Cry4 interaction partners. Six proteins were found to be particularly promising candidates for interacting with European robin Cry4. The identified genes code for guanine nucleotide-binding protein G(t) subunit alpha-2 (GNAT2), long-wavelength-sensitive opsin (LWS, also called iodopsin), guanine nucleotide-binding protein subunit gamma 10 (GNG10), potassium voltage-gated channel subfamily V member 2 (KCNV2), retinol binding protein 1 (RBP1) and retinal G protein-coupled receptor (RGR). All genes are known to be expressed in vertebrate retinae of different species. We conclude by discussing putative signalling pathways that could connect cryptochrome 4 to one or more of these 6 candidates.
Development of a polyvinyl alcohol/sodium alginate hydrogel-based scaffold incorporating bFGF-encapsulated microspheres for accelerated wound healing
Authors: Bahadoran Maedeh , Shamloo Amir , Nokoorani Yeganeh Dorri 
Published: April 2020

AbstractIn the present study, a hybrid microsphere/hydrogel system, consisting of polyvinyl alcohol (PVA)/sodium alginate (SA) hydrogel incorporating PCL microspheres is introduced as a skin scaffold to accelerate wound healing. The hydrogel substrate was developed using the freeze-thawing method, and the proportion of the involved polymers in its structure was optimized based on the in-vitro assessments. The bFGF-encapsulated PCL microspheres were also fabricated utilizing the double-emulsion solvent evaporation technique. The achieved freeze-dried hybrid system was then characterized by in-vitro and in-vivo experiments. The results obtained from the optimization of the hydrogel showed that increasing the concentration of SA resulted in a more porous structure, and higher swelling ability, elasticity and degradation rate, but decreased the maximum strength and elongation at break. The embedding of PCL microspheres into the optimized hydrogel structure provided sustained and burst-free release kinetics of bFGF. Besides, the addition of drug-loaded microspheres led to no significant change in the degradation mechanism of the hydrogel substrate; however, it reduced its mechanical strength. Furthermore, the MTT assay represented no cytotoxic effect for the hybrid system. The in-vivo studies on a burn-wound rat model, including the evaluation of the wound closure mechanism, and histological analyses indicated that the fabricated scaffold efficiently contributed to promoting cell-induced tissue regeneration and burn-wound healing.
Risk of Corneal Ulcer in Patients with Diabetes Mellitus: A Retrospective Large-Scale Cohort Study
Authors: Chang Yuh-Shin , Tai Ming-Cheng , Ho Chung-Han , Chu Chin-Chen , Wang Jhi-Joung , Tseng Sung-Huei , Jan Ren-Long 
Published: April 2020

AbstractThis nationwide, retrospective, matched cohort study was designed to investigate the risk of corneal ulcer in patients with diabetes mellitus (DM). It included 238,701 patients with DM, recruited between 2003 and 2005 from the Longitudinal Cohort of Diabetes Patients database. The control group included the same number of age- and sex-matched non-DM patients selected from the Taiwan Longitudinal Health Insurance Database, 2000. The data of each patient were collected from the index date until December 2013. The incidence of corneal ulcer was compared between the two groups. In total, 2,549 patients with DM and 1,988 controls developed corneal ulcer during the follow-up period, resulting in an incidence rate for corneal ulcers that was 1.27 times (95% confidence interval [CI] = 1.20–1.35; P < 0.001) higher in patients with DM than in controls. After adjustment for potential confounders, including hyperlipidemia, hypertension, congestive heart failure, coronary artery disease, and chronic renal disease, patients with DM were 1.31 times (95% CI, 1.24–1.40; P < 0.05) more likely than the cohort to develop corneal ulcers. In conclusion, this study shows that DM increases the risk of corneal ulcer. Therefore, close collaboration between ophthalmologists and endocrinologists is important to ensure timely ophthalmology visits.
Graphene Oxide Papers in Nanogenerators for Self-Powered Humidity Sensing by Finger Tapping
Authors: Ejehi Faezeh , Mohammadpour Raheleh , Asadian Elham , Sasanpour Pezhman , Fardindoost Somayeh , Akhavan Omid 
Published: April 2020

AbstractTriboelectric nanogenerators (TENGs) offer an emerging market of self-sufficient power sources, converting the mechanical energy of the environment to electricity. Recently reported high power densities for the TENGs provide new applications opportunities, such as self-powered sensors. Here in this research, a flexible graphene oxide (GO) paper was fabricated through a straightforward method and utilized as the electrode of TENGs. Outstanding power density as high as 1.3 W.m−2, an open-circuit voltage up to 870 V, and a current density of 1.4 µA.cm−2 has been extracted in vertical contact-separation mode. The all-flexible TENG has been employed as a self-powered humidity sensor to investigate the effect of raising humidity on the output voltage and current by applying mechanical agitation in two forms of using a tapping device and finger tapping. Due to the presence of superficial functional groups on the GO paper, water molecules are inclined to be adsorbed, resulting in a considerable reduction in both generated voltage (from 144 V to 14 V) and current (from 23 µA to 3.7 µA) within the range of relative humidity of 20% to 99%. These results provide a promising applicability of the first suggested sensitive self-powered GO TENG humidity sensor in portable/wearable electronics.
Validation of Salivary Markers, IL-1β, IL-8 and Lgals3bp for Detection of Oral Squamous Cell Carcinoma in an Indian Population
Authors: Singh Prerana , Verma Jitendra K. , Singh Jayant Kumar 
Published: April 2020

AbstractEarly detection and easier follow-up of oral squamous cell carcinoma (OSCC) would significantly improve the morbidity and mortality associated with it. With newer technologies, it has become possible to validate cancer biomarkers in saliva with high sensitivity and specificity. There is however a need to further validate these biomarkers in cohorts of different ethnic groups. Our objective was to validate previously evaluated salivary biomarkers in Indian population. The study enrolled 117 patients. These were grouped into subcatergories of 31 early (TNMstage I-II) and 27 late-stage OSCC (TNM stage III-IV), 30 PMOD and 29 post-treatment patients. There were 42 control subjects. We evaluated 3 protein markers, IL-1β, IL-8 and LGALS3BP using ELISA, from unstimulated saliva samples. Statistical analysis was done to calculate p-value, ROC, AUC, sensitivity, and specificity. Protein markers IL-1β and IL-8 were significantly elevated (p < 0.05) in OSCC patients. Though the markers could not discriminate PMOD and post-treatment subjects from controls, they proved to be significantly discriminatory between OSCC and controls. Both these markers were especially strong discriminators of late stage OSCC (stage III-IV). IL-1β had the most statistically significant discriminative power (AUC = 0.9017) in late-stage OSCC followed by IL-8 (AUC = 0.7619). Although LGALS3BP was not found to be significantly elevated in late stage OSCC patients, but it was a significant discriminator of early stage OSCC (stage I-II) with p-value = 0.0008 and AUC = 0.7296. These salivary biomarkers have been discovered and validated in other ethnic groups earlier. Hence, the fact that these markers were discriminatory in Indian population too, strengthens the possibility of using these salivary biomarkers as screening tools in different ethnic cohorts. Such trials would potentiate use of a non-invasive tool, like saliva for diagnosis and follow-up of oral cancer.
Clinical significance of post-liver transplant hepatitis E seropositivity in high prevalence area of hepatitis E genotype 3: a prospective study
Authors: Komolmit Piyawat , Oranrap Vinita , Suksawatamnuay Sirinporn , Thanapirom Kessarin , Sriphoosanaphan Supachaya , Srisoonthorn Nunthiya , Posuwan Nawarat , Thongmee Thanunrat , Treeprasertsuk Sombat , Poovorawan Yong 
Published: April 2020

AbstractHigh hepatitis E (HEV) seroprevalence has been reported in the general population and in post-liver transplant (LT) cases in several regions, including Thailand, with genotype 3 being a predominant genotype. We hypothesized that HEV might persist at a subclinical level and might pose clinical risks in the post-LT period. We performed a cross-sectional study with 108 post-LT patients and found an IgG seroprevalence of 55.6%. Subsequently, 91 cases without clinical evidence of HEV-related hepatitis were enrolled in 1 year of prospective follow-up to determine clinical status, serologies and serum/feces HEV RNA every 4 months. HEV RNA was detected, indicating subclinical infections in patients with or without seropositivity, with an annual incidence of 7.7%. Our results suggest that subclinical HEV infection exists among LT patients in this high-prevalence area. Thus, clinicians should be aware of the possibility of disease reemergence and HEV viral transmission in LT patients.
Sparse estimation of mutual information landscapes quantifies information transmission through cellular biochemical reaction networks
Authors: Sarkar Swarnavo , Tack Drew , Ross David 
Published: April 2020

AbstractMeasuring information transmission from stimulus to response is useful for evaluating the signaling fidelity of biochemical reaction networks (BRNs) in cells. Quantification of information transmission can reveal the optimal input stimuli environment for a BRN and the rate at which the signaling fidelity decreases for non-optimal input probability distributions. Here we present sparse estimation of mutual information landscapes (SEMIL), a method to quantify information transmission through cellular BRNs using commonly available data for single-cell gene expression output, across a design space of possible input distributions. We validate SEMIL and use it to analyze several engineered cellular sensing systems to demonstrate the impact of reaction pathways and rate constants on mutual information landscapes.
Investigation of the impact of magnesiumversustitanium implants on protein composition in osteoblast by label free quantification
Authors: Omidi M , Ahmad Agha N , Müller A , Feyerabend F , Helmholz H , Willumeit-Römer R , Schlüter H , Luthringer-Feyerabend B J C 
Published: April 2020

AbstractMetallic implant biomaterials predominate in orthopaedic surgery. Compared to titanium-based permanent implants, magnesium-based ones offer new possibilities as they possess mechanical properties closer to the ones of bones and they are biodegradable. Furthermore, magnesium is more and more considered to be “bioactive” i.e., able to elicit a specific tissue response or to strengthen the intimate contact between the implant and the osseous tissue. Indeed, several studies demonstrated the overall beneficial effect of magnesium-based materials on bone tissue (in vivo and in vitro). Here, the direct effects of titanium and magnesium on osteoblasts were measured on proteomes levels in order to highlight metal-specific and relevant proteins. Out of 2100 identified proteins, only 10 and 81 differentially regulated proteins, compare to the control, were isolated for titanium and magnesium samples, respectively. Selected ones according to their relationship to bone tissue were further discussed. Most of them were involved in extracellular matrix maturation and remodelling (two having a negative effect on mineralisation). A fine-tuned balanced between osteoblast maturation, differentiation and viability was observed.
Gut microbiota in coronary artery disease: a friend or foe?
Authors: Zhang Bo , Wang Xinxin , Xia Ran , Li Chunsheng 
Published: April 2020

Abstract
There is a growing interest in the role of gut microbiota in the pathophysiology of several diseases, including coronary artery diseases (CAD). Gut microorganisms may produce beneficial effects in myocardial ischemia either directly in the form of exogenous administration or indirectly by acting on fiber-rich food to produce important cardioprotective components. The harmful effects of gut microbiota in CAD are due to alteration in their composition with a significant decrease in Bacteroidetes and an increase in Firmicutes, Escherichia, Shigella, and Enterococcus. The altered microbiota may produce potentially toxic metabolites, including trimethylamine-N-oxide (TMAO). Indeed, the fasting plasma levels of TMAO are directly correlated to increased risk of major cardiovascular events in CAD patients, and it is proposed as a potential biomarker to predict the onset of major cardiovascular events. It is concluded that the change in the composition of gut microbiota in CAD patients may predispose to more harmful effects. However, exogenous delivery of probiotics may overcome the detrimental effects of myocardial ischemia.
A family portrait of disk inner rims around Herbig Ae/Be stars
Authors: Kluska J. , Berger J.-P. , Malbet F. , Lazareff B. , Benisty M. , Le Bouquin J.-B. , Absil O. , Baron F. , Delboulbé A. , Duvert G. , Isella A. , Jocou L. , Juhasz A. , Kraus S. , Lachaume R. , Ménard F. , Millan-Gabet R. , Monnier J. D. , Moulin T. , Perraut K. , Rochat S. , Pinte C. , Soulez F. , Tallon M. , Thi W.-F. , Thiébaut E. , Traub W. , Zins G. 
Published: April 2020

Context. The innermost astronomical unit (au) in protoplanetary disks is a key region for stellar and planet formation, as exoplanet searches have shown a large occurrence of close-in planets that are located within the first au around their host star.
Aims. We aim to reveal the morphology of the disk inner rim using near-infrared interferometric observations with milli-arcsecond resolution provided by near-infrared multitelescope interferometry.
Methods. We provide model-independent reconstructed images of 15 objects selected from the Herbig AeBe survey carried out with PIONIER at the Very Large Telescope Interferometer, using the semi-parametric approach for image reconstruction of chromatic objects. We propose a set of methods to reconstruct and analyze the images in a consistent way.
Results. We find that 40% of the systems (6/15) are centrosymmetric at the angular resolution of the observations. For the rest of the objects, we find evidence for asymmetric emission due to moderate-to-strong inclination of a disk-like structure for ~30% of the objects (5/15) and noncentrosymmetric morphology due to a nonaxisymmetric and possibly variable environment (4/15, ~27%). Among the systems with a disk-like structure, 20% (3/15) show a resolved dust-free cavity. Finally, we do not detect extended emission beyond the inner rim.
Conclusions. The image reconstruction process is a powerful tool to reveal complex disk inner rim morphologies, which is complementary to the fit of geometrical models. At the angular resolution reached by near-infrared interferometric observations, most of the images are compatible with a centrally peaked emission (no cavity). For the most resolved targets, image reconstruction reveals morphologies that cannot be reproduced by generic parametric models (e.g., perturbed inner rims or complex brightness distributions). Moreover, the nonaxisymmetric disks show that the spatial resolution probed by optical interferometers makes the observations of the near-infrared emission (inside a few au) sensitive to temporal evolution with a time-scale down to a few weeks. The evidence of nonaxisymmetric emission that cannot be explained by simple inclination and radiative transfer effects requires alternative explanations, such as a warping of the inner disks. Interferometric observations can therefore be used to follow the evolution of the asymmetry of those disks at an au or sub-au scale.
Development of MALDI-TOF mass spectrometry for the identification of lice isolated from farm animals
Authors: Ouarti Basma , Laroche Maureen , Righi Souad , Meguini Mohamed Nadir , Benakhla Ahmed , Raoult Didier , Parola Philippe 
Published: April 2020

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is now routinely used for the rapid identification of microorganisms isolated from clinical samples and has been recently successfully applied to the identification of arthropods. In the present study, this proteomics tool was used to identify lice collected from livestock and poultry in Algeria. The MALDI-TOF MS spectra of 408 adult specimens were measured for 14 species, includingBovicola bovis, B. ovis, B. caprae, Haematopinus eurysternus, Linognathus africanus, L. vituli, Solenopotes capillatus,Menacanthus stramineus,Menopon gallinae, Chelopistes meleagridis,Goniocotes gallinae,Goniodes gigas, Lipeurus caponisand laboratory rearedPediculus humanus corporis. Good quality spectra were obtained for 305 samples. Spectral analysis revealed intra-species reproducibility and inter-species specificity that were consistent with the morphological classification. A blind test of 248 specimens was performed against the in-lab database upgraded with new spectra and validated using molecular tools. With identification percentages ranging from 76% to 100% alongside high identification scores (mean = 2.115), this study proposes MALDI-TOF MS as an effective tool for discriminating lice species.