bluuebunny/crossref_metadata_2025_split huggingface dataset:
Diabetes promotes invasive pancreatic cancer by increasing systemic and tumour carbonyl stress in KrasG12D/+ mice
Authors: Menini Stefano , Iacobini Carla , de Latouliere Luisa , Manni Isabella , Vitale Martina , Pilozzi Emanuela , Pesce Carlo , Cappello Paola , Novelli Francesco , Piaggio Giulia , Pugliese Giuseppe 
Published: August 2020

AbstractBackgroundType 1 and 2 diabetes confer an increased risk of pancreatic cancer (PaC) of similar magnitude, suggesting a common mechanism. The recent finding that PaC incidence increases linearly with increasing fasting glucose levels supports a central role for hyperglycaemia, which is known to cause carbonyl stress and advanced glycation end-product (AGE) accumulation through increased glycolytic activity and non-enzymatic reactions. This study investigated the impact of hyperglycaemia on invasive tumour development and the underlying mechanisms involved.MethodsPdx1-Cre;LSL-KrasG12D/+mice were interbred with mitosis luciferase reporter mice, rendered diabetic with streptozotocin and treated or not with carnosinol (FL-926-16), a selective scavenger of reactive carbonyl species (RCS) and, as such, an inhibitor of AGE formation. Mice were monitored for tumour development by in vivo bioluminescence imaging. At the end of the study, pancreatic tissue was collected for histology/immunohistochemistry and molecular analyses. Mechanistic studies were performed in pancreatic ductal adenocarcinoma cell lines challenged with high glucose, glycolysis- and glycoxidation-derived RCS, their protein adducts AGEs and sera from diabetic patients.ResultsCumulative incidence of invasive PaC at 22 weeks of age was 75% in untreated diabetic vs 25% in FL-926-16-gtreated diabetic and 8.3% in non-diabetic mice. FL-926-16 treatment suppressed systemic and pancreatic carbonyl stress, extracellular signal-regulated kinases (ERK) 1/2 activation, and nuclear translocation of Yes-associated protein (YAP) in pancreas. In vitro, RCS scavenging and AGE elimination completely inhibited cell proliferation stimulated by high glucose, and YAP proved essential in mediating the effects of both glucose-derived RCS and their protein adducts AGEs. However, RCS and AGEs induced YAP activity through distinct pathways, causing reduction of Large Tumour Suppressor Kinase 1 and activation of the Epidermal Growth Factor Receptor/ERK signalling pathway, respectively.ConclusionsAn RCS scavenger and AGE inhibitor prevented the accelerating effect of diabetes on PainINs progression to invasive PaC, showing that hyperglycaemia promotes PaC mainly through increased carbonyl stress. In vitro experiments demonstrated that both circulating RCS/AGEs and tumour cell-derived carbonyl stress generated by excess glucose metabolism induce proliferation by YAP activation, hence providing a molecular mechanism underlying the link between diabetes and PaC (and cancer in general).
Using electroacupuncture to recover muscle strength in patients with knee osteoarthritis after total knee arthroplasty: a study protocol for a double-blinded, randomized, and placebo-controlled trial
Authors: Xu Hui , Kang Bingxin , Li Yulin , Xie Jun , Sun Songtao , Zhong Sheng , Gao Chenxin , Xu Xirui , Zhao Chi , Qiu Guowei , Xiao Lianbo 
Published: August 2020

Abstract
Background
Total knee arthroplasty (TKA) is a gold standard for patients with terminal term gonarthrosis for reducing pain, correcting deformities, and regaining stability. However, post-TKA muscle strength recovery is often difficult. Although electroacupuncture (EA) enhances lower extremity muscle strength of the lower extremity, there is limited evidence regarding its effect on lower extremity muscle strength in post-TKA patients. Consequently, this trial intends to evaluate the efficacy of post-TKA EA on the recovery of lower extremity muscle strength, specifically, during the early post-TKA period.

Methods/design
This is a double-blinded, randomized, and controlled trial. It will be conducted between August 2020 and December 2020. Ninety-four participants with KOA who have undergone unilateral TKA will be randomized into a treatment (EA) group and a control (sham EA) group. The former and latter groups will receive EA and sham EA, respectively, at ST37, ST36, SP10, and SP9 acupoints. The participants will undergo ten treatment sessions over 2 weeks (5 sessions per week). The primary outcomes will include changes in muscle strength and the Hospital for Special Surgery score at the second week from baseline (pre-op 1 day or POD 3). The secondary outcomes will include a 4-m walk test, numerical rating scale score, the Hamilton Anxiety Scale score, and additional analgesia use. Additional outcomes will include the incidence of analgesia-related side effects and the participant satisfaction rate. Participant blinding will also be assessed where they will be asked to guess whether they received EA after the latest intervention. Adverse EA events will be documented and assessed throughout the trial.

Discussion
EA is helpful for post-TKA recovery and enhancement of lower limb muscle strength.

Trial registration
Chinese Clinical Trial Registry ChiCTR1900027806. Registered on 29 November 2019
Resistance to commonly used insecticides and underlying mechanisms of resistance in Aedes aegypti (L.) from Sri Lanka
Authors: Fernando H. Sachini D. , Saavedra-Rodriguez Karla , Perera Rushika , Black William C. , De Silva B. G. D. Nissanka Kolitha 
Published: August 2020

Abstract
Background
Drastic increases of dengue fever (DF) over the past few years have prompted studies on the development of resistance to insecticides in the mosquito vector, Aedes aegypti (Linnaeus). In Sri Lanka control of the vector population is essentially achieved using larvicides (temephos) and adulticides (principally pyrethroids). The present study investigates resistance to commonly used insecticides and underlying mechanisms of Ae. aegypti in selected sites in Sri Lanka.

Methods
In this study, susceptibility to three commonly used adulticides (malathion, permethrin and deltamethrin) and the larvicide temephos were tested for Ae. aegypti sampled from five localities in Sri Lanka using WHO dose diagnostics tests. In addition, we performed dose-response tests for permethrin to determine lethal concentrations (LCs) with CDC bottle bioassays. An assessment of the activity of metabolic detoxifying enzymes (multifunction oxidases (MFOs), glutathione S-transferases (GSTs) and esterases) and determination of frequency of the kdr mutations (F1534C, V1016G and S989P) were also carried out to ascertain the associated resistance mechanisms. Kdr genotype frequencies were compared with samples collected from the same sites in 2015 to determine the change of allele frequencies over the years.

Results
The present study revealed resistance in all Ae. aegypti populations studied, with low mortality percentages for both permethrin (10–89%) and deltamethrin (40–92%). Dose response tests revealed highest resistance ratios (RR) for permethrin and temephos from Colombo district whereas Puttalum district exhibited the lowest. High frequencies of the 1534C allele (0.052–0.802) were found in the study sites in 2017. Comparison with samples collected in 2015 revealed a substantial increase in this allele. The activity of MFOs and p-nitro phenyl-acetate esterase was significantly greater in most Sri Lankan populations in comparison to that of the New Orleans (NO) susceptible strain. In contrast, the activity of α-esterase and β-esterase was similar or lower than that in the NO strain.

Conclusions
Aedes aegypti from Sri Lanka is resistant to pyrethroid insecticides showing rapid selection for kdr mutations and varying metabolic mechanisms. Continued monitoring of vector populations is crucial to mitigate the development of resistance to commonly used insecticides and in turn, controlling the vector population.
MylynSDP — Process - aware artifact filtering based on interest
Authors: Portugal Ivens , Oliveira Toacy , Alencar Paulo , Cowan Donald 
Published: August 2020

AbstractA software development process is used by software engineers to guide their activities during all phases of the software product development. When executing a software development process, software engineers may lose time and effort while searching for artifacts or changing contexts. This happens, for example, when they need to search for a specific code file in a list of hundreds of files or when they interrupt an activity to execute another but forget specific details and need to re-execute searches related to the previous activity. This impacts their productivity negatively, because extra time and effort are spent into non-productive work. Therefore, automated assistance is required to mitigate or avoid these issues. The Degree of Interest (DOI) function infers an element’s importance in a context, helping software engineers to handle many artifacts. Mylyn, an Eclipse IDE plugin, uses a DOI function on Java documents to assist programmers when looking for code documents during development. However, Mylyn’s DOI function is limited to the implementation phase of software processes and relies on manual task creation. This paper presents MylynSDP, a software Process-aware extension to Mylyn’s DOI function. MylynSDP’s DOI function infers an artifact’s importance during an activity and filters uninteresting artifacts, reducing the time taken to search items and improving productivity. Mylyn code was augmented, and an evaluation study was performed. Seven subjects executed a software process with many artifacts. Exercise times were recorded for productivity analysis. Subjects answered a Technology Acceptance Model (TAM) questionnaire. New task and artifact creation wizards link tasks and artifacts to specification activities and artifacts, respectively. A new interaction event handles context creation, and the DOI function was extended to other software process phases. Exercise time reduction shows a productivity increase. TAM questionnaire answers show a positive overall willingness to adopt MylynSDP and provide evidence that using a DOI function in different software process phases increases productivity. This work advances the state of the art in software engineering by providing additional methods to support artifact search and discovery, context change management, and artifact relevance mechanisms.
Evolution and development of three highly specialized floral structures of bee-pollinated Phalaenopsis species
Authors: Pramanik Dewi , Dorst Nemi , Meesters Niels , Spaans Marlies , Smets Erik , Welten Monique , Gravendeel Barbara 
Published: August 2020

Abstract
Background
Variation in shape and size of many floral organs is related to pollinators. Evolution of such organs is driven by duplication and modification of MADS-box and MYB transcription factors. We applied a combination of micro-morphological (SEM and micro 3D-CT scanning) and molecular techniques (transcriptome and RT-PCR analysis) to understand the evolution and development of the callus, stelidia and mentum, three highly specialized floral structures of orchids involved in pollination. Early stage and mature tissues were collected from flowers of the bee-pollinated Phalaenopsis equestris and Phalaenopsis pulcherrima, two species that differ in floral morphology: P. equestris has a large callus but short stelidia and no mentum, whereas P. pulcherrima has a small callus, but long stelidia and a pronounced mentum.

Results
Our results show the stelidia develop from early primordial stages, whereas the callus and mentum develop later. In combination, the micro 3D-CT scan analysis and gene expression analyses show that the callus is of mixed petaloid-staminodial origin, the stelidia of staminodial origin, and the mentum of mixed sepaloid-petaloid-staminodial origin. SEP clade 1 copies are expressed in the larger callus of P. equestris, whereas AP3 clade 1 and AGL6 clade 1 copies are expressed in the pronounced mentum and long stelidia of P. pulcherrima. AP3 clade 4, PI-, AGL6 clade 2 and PCF clade 1 copies might have a balancing role in callus and gynostemium development. There appears to be a trade-off between DIV clade 2 expression with SEP clade 1 expression in the callus, on the one hand, and with AP3 clade 1 and AGL6 clade 1 expression in the stelidia and mentum on the other.

Conclusions
We detected differential growth and expression of MADS box AP3/PI-like, AGL6-like and SEP-like, and MYB DIV-like gene copies in the callus, stelidia and mentum of two species of Phalaenopsis, of which these floral structures are very differently shaped and sized. Our study provides a first glimpse of the evolutionary developmental mechanisms driving adaptation of Phalaenopsis flowers to different pollinators by providing combined micro-morphological and molecular evidence for a possible sepaloid–petaloid–staminodial origin of the orchid mentum.
Non-classical presentation of vitamin D deficiency: a case report
Authors: Ibrahim Mohanad Kamaleldin Mahmoud , Elnimeiri Mustafa Khidir Mustafa 
Published: August 2020

Abstract
Background
Vitamin D is a fat-soluble vitamin; vitamin D is essential to sustain health and it protects against osteoporosis. It is crucial to the human body’s physiology in terms of muscular movement and neurological signal transmission, and to the immune system in defense against invading pathogens.

Case presentation
This was a case of a 26-year-old Sudanese woman who presented with a 2-year history of anosmia, recurrent nasal polyps, back pain, and chronic fatigue. She was diagnosed as having a case of vitamin D deficiency and responded well to treatment.

Conclusion
There is an association between vitamin D deficiency and recurrent allergic nasal conditions.
Two-year long safety and efficacy of deferasirox film-coated tablets in patients with thalassemia or lower/intermediate risk MDS: phase 3 results from a subset of patients previously treated with deferasirox in the ECLIPSE study
Authors: Tartaglione Immacolata , Origa Raffaella , Kattamis Antonis , Pfeilstöcker Michael , Gunes Sibel , Crowe Susanne , Fagan Niamh , Vincenzi Beatrice , Ruffo Giovan Battista 
Published: August 2020

Abstract
Background
Despite the proven benefits of iron chelation therapy (ICT) in the management of chronic iron overload and related complications, compliance to long-term ICT is challenging. Results from the ECLIPSE study, an open-label, randomized, multicenter, 2-arm, phase 2 study evaluated the safety of deferasirox dispersible tablet and film-coated tablet (FCT) formulations in patients with transfusion-dependent thalassemia (TDT) or very low, low, or intermediate risk myelodysplastic syndrome (MDS) treated over 24 weeks.

Methods
The aim of the current study (a 2-year, open-label, multicenter, single-arm, phase 3 study) is to evaluate the long-term safety and efficacy of deferasirox FCT in a subset of patients with TDT or lower/intermediate-risk MDS treated for 2 years after the completion of 24 weeks of treatment with deferasirox in the ECLIPSE phase 2 study.

Results
Of 53 patients enrolled, 34 (64.2%) completed treatment and study. Adverse events (AEs) reported in most patients (~ 70%) were of mild to moderate severity. Headache and diarrhea were the most frequently (> 25%) reported AEs. None of the serious AEs (including 1 death) were considered treatment related. No new safety signal was identified, and long-term safety of deferasirox FCT was consistent with the known safety profile of deferasirox. No major concerns associated with gastrointestinal tolerability, renal safety, or hematological abnormalities (thrombocytopenia/neutropenia) were reported during the 2 years. Patients receiving deferasirox FCT had a treatment compliance (by pill count) of ~ 90% and persistence (continuous use for ≥ 30 days) of > 95%. Reduction in serum ferritin level was almost consistent starting from week 2 across all post-baseline time points (relative reduction: month 6, 19%; month 12, 29%).

Conclusions
The results from this 2-year interventional study suggest that the recommended dosing of deferasirox FCT, with better tolerability, palatability, and compliance, offers a favorable option of ICT for long-term management of iron overload and associated complications in TDT.
Trial registration ClinicalTrials.gov, NCT02720536. Registered 28 March 2016, https://www.clinicaltrials.gov/ct2/show/NCT02720536
Massive migration promotes the early spread of COVID-19 in China: a study based on a scale-free network
Authors: Song Wen-Yu , Zang Pan , Ding Zhong-Xing , Fang Xin-Yu , Zhu Li-Guo , Zhu Ya , Bao Chang-Jun , Chen Feng , Wu Ming , Peng Zhi-Hang 
Published: August 2020

Abstract
Background
The coronavirus disease 2019 (COVID-19) epidemic met coincidentally with massive migration before Lunar New Year in China in early 2020. This study is to investigate the relationship between the massive migration and the coronavirus disease 2019 (COVID-19) epidemic in China.

Methods
The epidemic data between January 25th and February 15th and migration data between Jan 1st and Jan 24th were collected from the official websites. Using the R package WGCNA, we established a scale-free network of the selected cities. Correlation analysis was applied to describe the correlation between the Spring Migration and COVID-19 epidemic.

Results
The epidemic seriousness in Hubei (except the city of Wuhan) was closely correlated with the migration from Wuhan between January 10 and January 24, 2020. The epidemic seriousness in the other provinces, municipalities and autonomous regions was largely affected by the immigration from Wuhan. By establishing a scale-free network of the regions, we divided the regions into two modules. The regions in the brown module consisted of three municipalities, nine provincial capitals and other 12 cities. The COVID-19 epidemics in these regions were more likely to be aggravated by migration.

Conclusions
The migration from Wuhan could partly explain the epidemic seriousness in Hubei Province and other regions. The scale-free network we have established can better evaluate the epidemic. Three municipalities (Beijing, Shanghai and Tianjin), eight provincial capitals (including Nanjing, Changsha et al.) and 12 other cities (including Qingdao, Zhongshan, Shenzhen et al.) were hub cities in the spread of COVID-19 in China.
The Samuel F. B. Morse statue in Central Park: scientific study and laser cleaning of a 19th-century American outdoor bronze monument
Authors: Basso Elena , Pozzi Federica , Reiley Matthew C. 
Published: August 2020

Abstract
Many of Central Park’s bronze statues were coated with lacquer as a protective measure between the late 1970s and early 1990s. In several cases, these coatings outlasted their performance life and were no longer adequately protecting the sculptures. In 2010, a cyclical campaign to remove and refurbish the coatings provided an opportunity to closely assess the condition of de-coated surfaces and to develop suitable, sustainable treatment approaches. The Samuel F. B. Morse statue, created in 1870 by Byron Pickett, commemorates the 19th-century painter and inventor known for the telegraphic code that bears his name. Initially, samples from this statue were collected on the field and analyzed with various techniques to characterize the degradation products that were heavily affecting its surface and the organic coatings used to protect the bronze. Various corrosion species, mainly sulfates and chlorides, were identified by X-ray powder diffraction (XRD), while pyrolysis-gas chromatography/mass spectrometry (Py-GC/MS) revealed that the organic coatings were based on mineral wax and acrylics. These results informed a decision to relocate the statue to a controlled workshop space to aid in the manipulation of the large figure and optimize cleaning by laser ablation. Treatment was monitored through three campaigns of analysis using portable X-ray fluorescence (pXRF) spectroscopy, performed on select locations, to characterize the extent of corrosion across the sculpture’s surface and monitor changes on the target areas throughout the cleaning. Following the initial phase of treatment, pXRF showed the substantial removal of iron- and lead-rich compounds from the brownish-toned coating and atmospheric pollution deposition. In the subsequent treatment phases, variations in the relative amounts of sulfates and chlorides were noted and further investigated through the examination of a cross section by means of scanning electron microscopy coupled with energy-dispersive X-ray spectroscopy (SEM/EDS). Based on the findings of iron-containing pigments in the organic coating, a decision to apply a new, similar, translucent, brown-toned coating containing such coloring materials was made to achieve the desired visual nuance for the finish and provide additional corrosion protection to the bronze. Scientific analysis on the Morse statue has helped characterize its materials, methods of fabrication, and current conditions on a microscopic scale; in addition, it has guided the means and methodologies of treatment and relays essential information useful for future preservation.
The color of risk protection orders: gun violence, gun laws, and racial justice
Authors: Swanson Jeffrey W. 
Published: August 2020

AbstractBackgroundExtreme risk protection order (ERPO) laws give municipal police officers new authority, through a civil restraining order, to remove firearms from people who are deemed to pose an imminent risk of causing serious harm to others or themselves. Despite the expected public safety benefit of ERPOs, it is possible that implicit racial bias could infect ERPO petitions, the court processes that authorize and extend the gun removal orders, or the behavior of the police in serving and enforcing them. How might potential racial disparities in ERPO implementation qualify the evidence that this intervention saves lives? What should gun violence prevention researchers and stakeholders do to promote racial justice?Main textThis commentary reflects upon an innovative and promising legal tool for gun violence prevention through the lens of racial justice concerns. Emerging research, guided by a public health paradigm, asks whether ERPOs save lives. But now is the time to pose other important questions as well. Preventing gun violence and mitigating the disproportionate impact of arrests and incarceration in communities of color are two important goals that may collide. The origin story of many U.S. firearm restrictions, and the continuing disparities in their enforcement and implementation, are intertwined with the legacy of systemic racial discrimination in policing and criminal justice in the United States. The public policy challenge of balancing risk and rights is increasingly fraught, especially as constituencies with a different interest in one or the other concern find themselves standing on opposite sides of a privilege chasm.ConclusionSaving lives from gun violence matters, but ensuring that the lives saved are also respected--free from racial oppression, afforded equal justice--also matters. This commentary is a call to include racial disparities impact as an essential outcome of interest for ERPO studies specifically, but more broadly for all public health law research studies at the intersection of firearm injury prevention, law enforcement, and criminal justice.