bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of transcranial direct current stimulation on sports performance for two profiles of athletes (power and endurance) (COMPETE): a protocol for a randomised controlled trial.
Authors: Grandperrin Yohan , Grosprêtre Sidney , Nicolier Magali , Gimenez Philippe , Vidal Chrystelle , Haffen Emmanuel , Bennabi Djamila 
Published: May 2020

Abstract
Background

Transcranial direct current stimulation (tDCS) is promising for improving motor and cognitive performance. Nevertheless, its mechanisms of action are unclear and need to be better characterised according to the stimulated brain area and the type of exercise performed.

Methods/design

This is a double-blind cross-over study, organised into two parts: the first is to assess the effects of tDCS on explosive performance (jump task) and the second is to assess the effects on endurance performance (cycling time trial task). Participants, who are recreationally active or athletes (cyclists, parkour practitioners), will receive two active tDCS sessions (over the left dorsolateral prefrontal cortex and right motor cortex) and one sham tDCS session (part A) or two daily tDCS sessions (one active sequence and one sham) over five days (part B). Motor and cognitive performance will be compared before and after the tDCS sessions (part A) and before and after the first session, after the last session and at day 12 and day 30 of each tDCS sequence (part B).

Discussion

This study investigates the acute and long-term effects of tDCS on the motor and cognitive performance in healthy subjects. It will try to evaluate if tDCS could be considered as a neuroenhancement technology according to the physical task investigated (endurance versus explosive).
Dexmedetomidine for prevention of postoperative pulmonary complications in patients after oral and maxillofacial surgery with fibular free flap reconstruction:a prospective, double-blind, randomized, placebo-controlled trial
Authors: Liu Yun , Zhu Xi , Zhou Dan , Han Fang , Yang Xu Dong 
Published: May 2020

Abstract
Background: Postoperative pulmonary complications (PPCs) are common and significant problems for oral and maxillofacial surgery patients. Dexmedetomidine (DEX), an α2-adrenoreceptor agonist, has been proven having lung protection effects. However, since now, there has not been final conclusion about whether DEX can reduce the incidence of PPCs. We hypothesize that, in oral and maxillofacial surgery with fibular free flap reconstruction patients, DEX may decrease the incidence of PPCs.Methods: This was a prospective, double-blind, randomized, placebo-controlled, single-centered trial with two parallel arms. A total of 160 patients at intermediate-to-high risk of PPCs undergoing oral and maxillofacial surgery with fibular free flap reconstruction and tracheotomy were enrolled and randomized to receive continuous infusion of either DEX or placebo (normal saline). 0.4 ug/kg of DEX was given over 10mins as an initial dose followed by a maintaining dose of 0.4 ug/kg/h till the second day morning after surgery. At the same time, the normal saline was administered a similar quantity. The primary outcome was the incidence of PPCs according to Clavien-Dindo score within 7 days after surgery. Results: The two groups had similar characteristics at baseline. 18(22.5%) of 80 patients administered DEX, and 32(40.0%) of 80 patient administered placebo experienced PPCs within the first 7 days after surgery (relative risk [RR] 0.563,95% confidence interval [CI] 0.346-0.916; P=0.017). In the first 7 days after surgery, the DEX group had a lower incidence of PPCs and a better postoperative survival probability (Log-rank test, P=0.019), and was less prone to occur PPCs (Cox regression, P=0.025, HR=0.516). When the total dose of DEX was more than 328μg, the patients were unlikely to have PPCs (ROC curve, AUC=0.614, P=0.009).Conclusions: For patients undergoing oral and maxillofacial surgery with fibular free flap reconstruction and tracheotomy who were at intermediate or high risk of developing PPCs, continuous infusion of DEX could decrease the occurrence of PPCs during the first 7 days after surgery and shorten the length of hospital stay after surgery, but did not increase the prevalence of bradycardia or hypotension. Trial registration: Chinese Clinical Trial Registry, www.chictr.org.cn, number: ChiCTR1800016153; Registered on May 15, 2018.
Feasibility and behavioral effects of prolonged static and dynamic standing as compared to sitting in older adults with type 2 diabetes mellitus
Authors: Marusic Uros , Muller Martijn , Alexander Neil , Bohnen Nicolaas 
Published: May 2020

Abstract
Background: Physical inactivity is prevalent in older adults with type 2 diabetes mellitus (T2DM) and may exacerbate their clinical symptoms. The aim of this study was to examine the feasibility of 4-hour regular versus more dynamic standing sessions while performing routine desktop activities as a non-exercise physical activity intervention in older adults with T2DM to increase non-exercise activity.Methods: Twelve older adult patients with T2DM (3 female; age 71 ± 4 years; BMI 34 ± 5 kg/m2) completed three sessions (baseline sitting followed by "static" or "dynamic" desktop standing sessions). Participants stood behind a regular height-adjustable desk in the "static" standing session. An upright dynamic standing desk, which provides cues to make small weight-shifting movements, was used for the "dynamic" standing session. Oxygen consumption, cognitive performance, as well as net standing duration, total movement activity, and musculoskeletal discomfort were assessed during all three sessions.Results: All participants were able to complete all sessions. Oxygen consumption and overall movements progressively increased from sitting to static and dynamic standing, respectively (p<0.001). The duration of breaks during standing (p=0.024) and rate of total musculoskeletal discomfort development (p=0.043) were lower in the dynamic standing compared to static standing sessions. There was no evidence of executive cognitive worsening during either standing session compared to sitting.Conclusions: Prolonged 4-hour standing as a simple non-exercise physical intervention is feasible in older adults with T2DM and may have metabolic (VO2 consumption) benefits. Increasing movement during desktop standing may offer incremental benefits compared to regular standing. Prolonged desktop standing might provide an effective intervention in T2DM older participants to target sedentariness.
What are the sociodemographic and gender determinants of non-fatal self-harm in older adult users and non-users of antidepressants? A national population-based study
Authors: Hedna Khedidja , Fastbom Johan , Skoog Ingmar , Hensing Gunnel , Waern Margda 
Published: May 2020

Abstract
Background Late-life self-harm (SH) is often linked to depression. However, very few studies have explored the role of other factors and their interaction with depression in the occurrence of late-life SH. The objective of this research was to examine sociodemographic and gender factors associated with non-fatal SH, in older adults with and without antidepressant therapy.Methods We used national longitudinal register data from a total cohort of all Swedish residents aged ≥75 years between 2006-2014 (N=1,413,806). Using personal identity numbers, we linked individuals' data from numerous national registers. We identified all those with at least one episode of non-fatal self-harm (regardless of level of intent to die) and matched 50 controls to each case. A nested case–control design was used to investigate sociodemographic factors associated with non-fatal SH in the total cohort and among antidepressant users and non-users. Risk factors were analysed in adjusted conditional logistic regression models for the entire cohort and by gender.Results In all, 2242 individuals had at least one episode of a non-fatal SH (980 men and 1262 women). Being unmarried was a risk factor for non-fatal SH in men but not in women. Among users of antidepressants, higher non-fatal SH risk was observed in those born outside the Nordic countries (IRR: 1.44; 95% CI: 1.11–1.86), whereas in AD non-users increased risk was seen in those from Nordic countries other than Sweden (IRR: 1.58; 95% CI: 1.08–2.29). Antidepressant users with higher education had an increased risk of non-fatal SH (IRR: 1.34; 95% CI: 1.12–1.61), in both men and women. Conclusions Foreign country of birth was associated with increased risk for non-fatal SH in older adults with and without AD therapies. Being married was a protective factor for non-fatal SH in men. The complex association between sociodemographic factors and use of antidepressants in the occurrence of self-harm in older men and women indicates the need for multifaceted tailored preventive strategies including healthcare and social services alike.
SHiNeMaS : A web tool dedicated to seed lots history, phenotyping and cultural practices
Authors: De Oliveira Yannick , Burlot Laura , Dawson Julie C. , Goldringer Isabelle , Madi Darkawi , Rivière Pierre , Steinbach Delphine , Frank Gaëlle Van , Thomas Mathieu 
Published: May 2020

Abstract
Motivation: In 2005, researchers from the French National Research Institute for Agriculture, Food and Environment (Institut national de recherche pour l'agriculture, l'alimentation et l'environnement, INRAE) started a collaboration with the French farmers' seed network Reseau Semences Paysannes (RSP) on bread wheat participatory breeding (PPB). The aims were: (1) to study on-farm management of crop diversity, (2) to develop population-varieties adapted to organic and low-inputs agriculture, (3) to co-develop tools and methods adapted to on-farm experiments. In this project, researchers and farmers' organizations needed to map the history and life cycle of the population-varieties using network formalism to represent relationships between seed lots. All this information had to be centralized and stored in a database. Results: We describe here SHiNeMaS (Seeds History and Network Management System) a web tool database. SHiNeMaS aims to provide useful interfaces to track seed lot history and related data (phenotyping, environment, cultural practices). Although SHiNeMaS has been developed in the context of a bread wheat participatory breeding program, the database has been designed to manage any kind and even multiple cultivated plant species. SHiNeMaS is available under A ero GPL licence and uses free technologies such as the Python language, Django framework or PostgreSQL database management system (DBMS). Conclusion: We developed SHiNeMaS, a web tool database, dedicated to the management of the history of seed lots and related data like phenotyping, environmental information and cultural practices. SHiNeMaS has been used in production in our laboratory for 5 years and farmers' organizations facilitators manage their own information in the system.
Characterization of Clostridium novyi isolated from a sow in a sudden death case in Korea
Authors: Jeong Chang-Gi , Seo Byoung-Joo , Nazki Salik , Jung Byung Kwon , Khatun Amina , Yang Myeon-Sik , Kim Seung-Chai , Noh Sang-Hyun , Shin Jae-Ho , Kim Bumseok , Kim Won-Il 
Published: May 2020

Abstract
Background: Multifocal spherical nonstaining cavities and gram-positive, rod-shaped, and endospore-forming bacteria were found in the liver of a sow that died suddenly. Clostridium novyi type B was identified and isolated from the sudden death case, and the isolate was characterized by molecular analyses and bioassays in the current study.Results: C. novyi was isolated from the liver of a sow that died suddenly and was confirmed as C. novyi type B by differential PCR. The C. novyi isolate fermented glucose and maltose and demonstrated lecithinase activity, and the cell-free culture supernatant of the C. novyi isolate exhibited cytotoxicity toward Vero cells, demonstrating that the isolate produces toxins. In addition, whole-genome sequencing of the C. novyi isolate was performed, and the complete sequences of the chromosome (2.29 Mbp) and two plasmids (134 and 68 kbp) were identified for the first time. Based on genome annotation, 7 genes were identified as glycosyltransferases, which are known as alpha toxins; 23 genes were found to be related to sporulation; 12 genes were found to be related to germination; and 20 genes were found to be related to chemotaxis.Conclusion: C. novyi type B was isolated from a sow in a sudden death case and confirmed by biochemical and molecular characterization. Various virulence-associated genes were identified for the first time based on whole-genome sequencing.
Network-based identification of biomarkers for colon adenocarcinoma
Authors: Hu Fuyan , Wang Qing , Yang Zhiyuan , Zhang Zeng , Liu Xiaoping 
Published: May 2020

Abstract
Background: As one of the most common cancers with high mortality in the world, we are still facing a huge challenge in the prevention and treatment of colon cancer. With the rapid development of high throughput technologies, new biomarkers identification for colon cancer has been confronted with the new opportunities and challenges.Methods: We firstly constructed functional networks for each sample of colon adenocarcinoma (COAD) by using a sample-specific network (SSN) method which can construct individual-specific networks based on gene expression profiles of a single sample. The functional genes and interactions were identified from the functional networks, respectively.Results: Classification and subtyping were used to test the function of the functional genes and interactions. The results of classification showed that the functional genes could be used as diagnostic biomarkers. The subtypes displayed different mechanisms, which were shown by the functional and pathway enrichment analysis for the representative genes of each subtype. Besides, subtype-specific molecular patterns were also detected, such as subtype-specific clinical and mutation features. Finally, 12 functional genes and 13 functional edges could serve as prognosis biomarkers since they were associated with the survival rate of COAD.Conclusions: In conclusion, the functional genes and interactions in the constructed functional network could be used as new biomarkers for COAD. And the subtypes could be used in realizing the personalized medicine of COAD.
Efficient generation of site-specific point mutations in cell lines by hyper active CBEs (hyCBEs)
Authors: Zhu Biyun , Chen Liang , Zhang Xiaohui , Li Dali 
Published: May 2020

Abstract
Cytidine base editors (CBEs) are powerful genetic tools which catalyze cytidine to thymidine conversion at specific genomic loci. Further improvement of the editing range and efficiency is critical for their broader applications. In this protocol, we demonstrated that fusion of the single-strand DNA binding domain (ssDBD) from Rad51 protein between Cas9n and cytidine deaminases, serial hyper CBEs (hyCBEs) were generated with substantially increased activity and an expanded editing window toward the protospacer adjacent motif (PAM) in cell lines. Moreover, hyeA3A-BE4max specifically generated a C-to-T conversion without inducing bystander mutations in the hemoglobin gamma (HBG) gene promoter to mimic a naturally occurring genetic variant for amelioration of β-hemoglobinopathy, suggesting the therapeutic potential of the improved base editors. This step-by-step protocol is related to the publication “Increasing the efficiency and targeting range of cytidine base editors through fusion of a single-strand DNA binding protein domain” in Nature Cell Biology.
Differential Influences of Social Support on App Use for Diabetes Self-Management – a Mixed Methods Approach
Authors: Brew-Sam Nicola , Chib Arul , Rossmann Constanze 
Published: May 2020

Abstract
Background The impact of social support on diabetes management and health outcomes has been investigated comprehensively, with recent studies examining social support delivered via digital technologies. This paper argues that social support has an impact on the use of diabetes technologies. Specifically, we postulate differences between the impact of healthcare professional versus non-professional (family/friends) support on mobile app use for diabetes self-management. Methods This research employed a triangulation of methods including exploratory semi-structured face-to-face interviews (N= 21, Study 1) and an online survey (N= 65, Study 2) with adult type 1 and type 2 diabetes patients. Thematic analysis (Study 1) was used to explore the relevance of social support (by professionals versus non-professionals) for diabetes app use. Binary logistic regression (Study 2) was applied to compare healthcare decision-making, healthcare-patient communication, and the support by the personal patient network as predictors of diabetes app use, complemented by other predictors from self-management and technology adoption theory. Results The interviews (Study 1) demonstrated that (technology-supported) shared decision-making and supportive communication by healthcare professionals depended on the medical specialty of attending physicians. The personal patient network was perceived as either facilitating or hindering the use of mHealth for self-management. Binary logistic regression (Study 2) showed that the specialty of the physician significantly predicted the use of diabetes apps, with supervision by diabetes specialists increasing the likelihood of app use (as opposed to general practitioners). In addition, specialist care positively related to a higher chance of shared decision-making and better physician-patient communication. The support by the personal patient network predicted diabetes app use in the opposite direction, with less family/friend support increasing the likelihood of app use. Conclusion The results emphasize the relevance of support by healthcare professionals and by the patient network for diabetes app use and disclose differences from the existing literature. In particular, we found that the use of diabetes apps may increase in the absence of social support by family or friends (e.g., compensation for lack of support), and that use of diabetes apps may decrease when such support is high (e.g., no perceived need to use technology). Implications for practice are discussed.
A unique type of fully covered metal stent for the management of post liver transplant biliary anastomotic strictures
Authors: Warner Ben , Harrison Phillip , Farman Muhammad , Devlin John , Reffitt David , El-Sherif Yasser , Khorsandi Shirin E , Prachalias Andreas , Cerisuelo Miriam Cortes , Menon Krish , Jassem Wayel , Sriniva Parthi , Vilca-Melendez Hector , Heneghan Michael , Heaton Nigel , Joshi Deepak 
Published: May 2020

Abstract
Background: We report our experience of treating anastomotic strictures using a novel type of fully covered metal stent (FCSEMS). This stent, known as the Kaffes Stent, is short-length with an antimigration waist and easily removable due to long retrieval wires deployed within the duodenum. Methods: 62 patients underwent ERCP and Kaffes stent insertion for post-transplant anastomotic strictures following confirmation of a stricture and biliary obstruction on MRCP. These patients were retrospectively analysed for immediate and longterm stricture resolution, improvement in symptoms and liver function tests (LFTs), stricture recurrence and complication rates. Results: 96% had immediate stricture resolution and 81% of patients continued to have long-term resolution (mean follow-up period was 548 days). Of the 16 patients with symptoms of biliary obstruction, 13 had resolution of their symptoms. A further 46 patients were asymptomatic, although 18 of these had cholestatic liver function tests. Overall, there was a significant improvement in LFTs. Complication rates were 15% with only one patient requiring biliary reconstruction. Conclusions: The Kaffes stent is effective and safe at resolving post liver transplant biliary anastomotic strictures.
Clinical Significance of MicroRNA-155 Regulated Autophagy and Apoptosis by targeting gene Rictor/Fos in Gastric Cancer Progression
Authors: Chen Gang , Li Yu , Ren Zhijian , Gu Yanmei , Tang Futian , Mao Jie , Zhu Junmin , Wang Ling , Li Yumin 
Published: May 2020

Abstract
BACKGROUND Microrna-155 plays an important role in the pathogenesis, progression and treatment of various cancers. It is abnormally expressed in gastric cancer, but its expression level, mechanism and significance are controversial in different studies. So we make the study to explore the expression level, significance and mechanism of microRNA-155 on gastric cancer. METHODS Target genes of microRNA-155 in TargetScan and mirDB databases were analyzed by Wayne Mapping, Enrichr database, String database, TIMER database. Forty-three pairs of cancer tissues and adjacent tissues were collected to extract total RNA and protein. Expression of MicroRNA-155, Rictor, Fos, Beclin1, LC3, caspase3 and caspase9 were measured by qRT-PCR and western blot. The relationship between gene expression and clinicopathological factors were analyzed. SPSS 23.0 was used for statistical analysis. RESULTS A total of 700 (miRDB) and 556 (TargetScan) target genes were obtained and 280 genes were in the intersection of Wayne Mapping, 49 of them had a target score of 90 or more. GO and KEGG analysis revealed that they were related to autophagy or apoptosis pathway. Rictor and Fos were selected as research objects. Thirty-two cases showed high microRNA-155 expression (group H) and 11 cases showed low expression (group L). Twelve patients had high Rictor expression and 31 patients had low expression; Thirteen cases had roughly normal Fos expression and 30 cases had low or negative expression; Thirty-three cases had high Beclin1/LC3 expression and 10 cases had low expression; Ten cases had high caspase3/caspase9 expression and 33 cases had low expression. According to the results of immunohistochemistry and western blot, Rictor, Fos, caspase3 and caspase9 were low expressed while Beclin1 and LC3 were high expressed in group H. However, all the six genes had no significant difference in group L. CONCLUSIONS The abnormal expression of microRNA-155 may indicate the occurrence of gastric cancer and its expression level is negatively correlated with clinical stage of cancer. The down-regulated expression of Rictor and Fos, enhancement of autophagy and weakening of apoptosis may be related to the over-expression of microRNA-155. MicroRNA-155 may promote the progression of gastric cancer by promoting autophagy and inhibiting apoptosis.
Correlation between White Blood Cell Count at Admission and Mortality in COVID-19 Patients: A Retrospective Study
Authors: Feng Xiaokai , Zhu Bin , Jiang Chunguo , Mi Song , Yang Liya , Zhao Zhigang , Zhang Yong , Zhang Liming 
Published: May 2020

Abstract
Background: Coronavirus disease-19 (COVID-19) has spread rapidly and has become a world health threaten. Its risk factors with death were still unknow. White blood cells (WBC) as a reflection of inflammation had play a vital role in COVID-19, however its level with death were still not know. Methods: In this retrospective, single-center study, all confirmed patients with COVID-19 on admission at West Branch of Union Hospital from Jan 29 to Feb 28, were collected and analyzed. Demographic and clinical data including laboratory examinations were analyzed and compared between recovery and death patients.Results: A total of 163 patients including 33 death cases were included in this study. Significant associations were found between WBC level and death (HR = 1.14, 95%CI: 1.09-1.20, p<0.001). The regression analysis results showed there was a significant association between WBC level and death (HR = 5.72, 95%CI: 2.21-14.82, p < 0.001) when use the second quartile as a cutoff value (> 6.16×10^9/L). The difference was still existing after we adjusting for confounding factors (HR = 6.26, 95%CI: 1.72-22.77, p = 0.005). In addition, Kaplan-meier survival analysis showed that there was a significant decline of the cumulative survival rate (p < 0.001) in those with WBC level ≥ 6.16×10^9/L.Conclusion: WBC at admission is significantly corelated with death in COVID-19 patients. Higher level of WBC should be given more attention in the treatment of COVID-19.