bluuebunny/crossref_metadata_2025_split huggingface dataset:
Masculinization of guppies (Poecilia reticulata) using water of coconut hybrid variety with the immersion method of pregnant female
Authors: Meiliana G , Sulmartiwi L , Lutfiyah L 
Published: February 2020

Abstract
This encourages monosex male guppies cultivation which can be done through sex reversal techniques, namely masculinization by adding safer natural ingredients. The purpose of this study was to determine the effect of immersion of pregnant guppies fish using water of coconut hybrid on the percentage of male larvae and determine the dose of coconut water and the duration of immersion. The research method used was the experimental method with Factorial Completely Randomized Design continued with DMRT. The results of immersion of pregnant guppies fish in a solution of water coconut hybrid with different dosages and the duration of immersion had a significant effect (P <0.05) on the percentage of male larvae. The dosage of 15 ml coconut water for 10 hours produced 66.90% male guppies fish larvae. Supporting parameters showed results at a dose of 15 ml with the duration immersion of 10 hours obtained survival of 100%. Water quality during immersion and maintenance of larvae, namely temperatures of 26 - 28 oC, pH 6.6 - 8.0 and DO 6.4 - 6.8 mg/L.
The effect of combination Bifidobacterium sp and Lactobacillus acidophilus probiotic on egg yolk cholesterol, HDL, and LDL
Authors: Lokapirnasari W P , Sahidu A M , Maslachah L , Yulianto A B , Najwan R 
Published: February 2020

Abstract
The purpose of this study to evaluate the effects of combination 0.5% Bifidobacterium sp.+0.5% Lactobacillus acidophilus on egg yolk cholesterol, High density lipoprotein (HDL) and low density lipoprotein (LDL) in hens. Methods: this research using completely randomized design. Sixty layers were randomized into three treatments, each treatment contains four replicates and each replicates contains five hens. Twenty five week-old Isa Brown layers were fed without probiotic (T0), 0.1% antibiotic growth promoters (T1), 0.5% Bifidobacterium sp.+0.5% L. acidophilus (T2) for 30 days. Result: the result showed that there was a significant difference between the control and all treatment groups on egg yolk cholesterol, HDL and LDL. Supplementation of 0.5% Bifidobacterium sp.+0.5% L. acidophilus probiotic decreased the egg yolk cholesterol and LDL (p<0.05) and increased HDL in egg yolk (p<0.05) levels. The lowest egg yolk cholesterol and LDL by supplementation 0.5% Bifidobacterium sp.+0.5% L. acidophilus probiotic (155.86 and 48.46 mg/dL) respectively, compared the control (224.0200 and 58.6300 mg/dL) and the highest egg yolk HDL by supplementation of 0.5% Bifidobacterium sp.+0.5% L. acidophilus probiotic (50.00 mg/dL) compared the control treatment (41.5900 mg/dL). The conclusion of this study was the supplementation of 0.5% Bifidobacterium sp.+0.5% L. acidophilus could improve egg yolk quality of hens.
Morphometric asymmetry of Barbodes binotatus (cyprinidae) collected from three different rivers in Java
Authors: Astuti S S , Hariati A M , Kusuma W E , Wiadnya D G R 
Published: February 2020

Abstract

Barbodes binotatus (Bleeker, 1859) is an endemic freshwater fish species that currently used for aquaculture. Good quality seed can be attained from cross-breed of parental stock from different geographical areas. This study aimed to distinguish morphological asymmetry of the species collected from three different rivers in Java: Sungai Tawang (East Java); Sungai Opak (Central Java); and Sungai Cisanggarung (West Java). Total 30 specimen were randomly sub-sampled from each river. Morphology, morphometry and meristic characters, based on 14 anatomical landmarks were measured and analyzed using Thin Plate Spline (TPSDIG), Symmetry-Asymmetry Geometric Analysis (SAGE) and Principal Component Analysis (PCA). Result of SAGE analysis showed that there were no significant asymmetries observed within, and between regions, respectively (p > 0.05). The result of PCA analysis however, showed qualitative differences in ratio between eye diameter (ED) versus head length (HL) (49%) and total length (TL) versus standard length (SL) (17%). Genetic (through DNA analysis) study need to be elaborated to see the differences more accurately.
Neobenedenia girellae infestation on cobia (Rachycentron canadum) in Hurun Bay Lampung, Indonesia
Authors: Putri R D B , Rivaie A R , Subekti S , Sari P D W 
Published: February 2020

Abstract
Cobia (Rachycentron canadum) is newly developed fish in Indonesia with an export value, however, the production activities of this fish continue to decrease due to fish massive mortality caused by disease. This study aimed to determine the predilection, prevalence, and intensity of Neobenedenia girellae infestation on cobia in floating net cages culture at Hurun Bay, Lampung. The sampling method used in this study was the purposive sampling method. Fish samples were taken at 20-30 cm as much as 50 fish, while 40-50 cm as much as 20 fish. Neobenedenia girellae were found on cobia eyes, head, and fins. Results showed the prevalence level in 20-30 cm fish was 30% and intensity level was 2.31 parasites/fish, while in 40-50 cm fish showed the prevalence level of 65% with the intensity of 1.47 parasites/fish.
Ecotourism development through legality of mangrove processed products dan river tracing in Cemara Beach, Banyuwangi, East Java, Indonesia
Authors: Setyaningrum E W , Erwanto Z , Prapti K P , Jayanti A L , Dewi A T K , Susanti H D 
Published: February 2020

Abstract
Mangrove ecotourisms in Cemara Beach Banyuwangi Regency continues to grow. Community groups develop Cemara Beach by utilizing mangroves as natural resources without reducing the value of conservation. Mangrove processed which have been produced is mangrove peyek, mangrove tea, and mangrove syrup. The problems founded is 1) legality of mangrove product like peyek, tea, and mangrove syrup, 2) The potential of Mangrove Rivers that have not been explored. The method used is training related to the legality of processed food products, as well as observation and documentation for the utilization of mangrove-surrounded rivers in Cemara Beach. The purpose of this is the people who are members of the Joint Business Group of Rejo Beach Pakis Banyuwangi Village, which has been consistent and committed to developing the Cemara Coast area through evaluation values. The approved results are training on food security, and that become the basis for the issuance of the legality of processed mangrove products that are certified as Household Products (P-IRT). Another result is an ecotourism attractions by traversing rivers using boats or called a mangrove tracing, where the river facilitates mangrove trees with a path to the river and sea or estuary.
The fertilization of Tor soro fish (Valenciennes, 1842) using post cryopreservation sperm: the effect of skim milk as a cryoprotectant
Authors: Harjanti E R , Abinawanto  , Arifin O Z , Kristanto A H 
Published: February 2020

AbstractTor soro, an endemic fish from North Sumatera, has a high economic value. Environmental damage causes a decreasing ofTor soropopulations and leads to the extinction of these fishes in their natural habitat. Cryopreservation is an efficient strategy that can be used to minimize the problem. The success of this approach depends on how effective it is. This study to evaluate the fertilization ofTor sorousing post cryopreservation spermatozoa 48 hours. The sperms collected by stripping, followed by the dilution using soluble liquid (fish ringer methanol 10%+skim milk) 1:9 comparison. The concentrations of the skim milk that is used in 0%, 5%, 10%, 15%, 20%, and 25%. The equilibration was carried out at the 5°C for 10 minutes before, then it kept frozen at the temperature of -10°C for 48 hours. The sperm thawing was carried out at the 40°C for 1 minute and one hundred egg cells (±1 g) were fertilized using 1.5ml of post cryopreservation sperm. The ANOVA test showed there was a fertility percentage effect of the skim milk on the frozen sperm. The 10% skim milk give an optimum with the highest fertility of (p<0.05) with 91.25±2.22% and motility (p<0.05) in 10% with 82.90±1.40%.
Honey effect on sperm motility of kancra fish (Tor soro Valenciennes, 1842) after 48 hours freezing
Authors: Putri B S D , Abinawanto  , Arifin O Z , Kristanto A H 
Published: February 2020

AbstractKancra (Tor soro) is a freshwater endemic fish found in specific place of Indonesia, with a drastic decrease in its population due to the varying gonad maturity times between males and females. However, the use of cryopreservation is effective for the synchronization of their gametes using cryoprotectants. Therefore, this study aimed to determine the effect of honey concentration (0%, 5%, 10%, 15%, 20%, and 25%) using 10% of methanol in the spermatozoa motility of kancra fish 48 hours after cryopreservation. The study was conducted in the Research and Development Institute for Freshwater Fish Genetic Resources (Balai Besar Perikanan Budidaya Ikan Tawar, BBPBAT), in Cijeruk, Bogor, West Java. The sperm were collected through the stripping method followed by the evaluation of spermatozoa motility 48 hours after cryopreservation. Furthermore, it was equilibrated for 10 minutes at a temperature of 5 ºC, frozen at 10 ºC for 48 hours, and thawed for 1 minute at 40 ºC. The data were analyzed using ANOVA followed by the Tukey test. Additionally, the ANOVA test showed that honey had effects (P<0.05) on the motility percentage after cryopreservation with a 5% optimum concentration using 85.97±1.91%.
The spermatozoa viability of kancra fish (Tor soro, Valenciennes 1842) 48-hour after freezing: effect of brown sugar as natural cryoprotectant
Authors: Pamungkas M A B , Abinawanto  , Arifin O Z , Kristanto A H 
Published: February 2020

Abstract
Kancra fish (Tor soro, Valenciennes 1842) is one of the endemic fish in Indonesia which population keeps decreasing due to degradation of the environment. Conservation is needed and cryopreservation is an effective strategy in this regard. Brown sugar as a natural cryoprotectant has the potential to substitute less eco-friendly synthetic cryoprotectant. The study was carried out to determine the viability of T. soro spermatozoa after 48 hours of cryopreservation using brown sugar. Sperm sample were collected through stripping method. The ejaculated sperm were diluted using the extender (brown sugar-fish ringer + methanol 10%, 1:9). The brown sugar concentration examined were 0%, 5%, 10%, 15%, 20%, and 25%, and the sample was equilibrated at 4 °C for 10 minutes, and frozen at -10 °C for 48 hours. The process was followed by thawing process ast 40 ºC for 60 seconds. Data were analyzed using ANOVA, followed by Tukey test. The results showed that brown sugar concentration of 15% significantly affects viability of the sperm after 48 hours of cryopreservation with 83.75±1.71% (p<0.05). However, a study is needed to determine the effectivity of brown sugar as a cryoprotectant.
Feed additive of curcuma flour (Curcuma xanthorhiza) in commercial feed to growth rate and feed efficiency of tambaqui (Colossoma macropomum)
Authors: Oktavianti I , Agustono  , Lamid M 
Published: February 2020

Abstract
Tambaqui (Colossoma macropomum) is much demand by the public as a consumption fish. Addition of curcuma in feed to increase fish appetite because it contains curcumin and essential oil. The purpose of this study was to determine the growth rate and food efficiency in tambaqui (Colossoma macropomun) with feed additives of curcuma flour (Curcumaxanthorhiza) on commercial feed. The research method used is the experimental method. The research design used was a Completely Randomized Design (CRD). Having 5 types of treatment with repeated 4 times. The treatment used was the addition of curcuma flour on commercial feed, namely P1 (2.5%), P2 (5%), P3 (7.5%), P4 (10%) and P0 (100% commercial feed). The main parameters observed were the growth rate and feed efficiency. The supporting parameters observed were water quality (temperature, pH, DO and ammonia). Data analysis using Analyze of Variance. The results of the feed additive of curcuma in commercial feed of tambaqui have the highest average value of growth rate in P0 treatment, but not significantly different (P> 0.05) with treatment P1, P2, P3 and P4. Feed efficiency values have the highest average value in P2 treatment, but not significantly different (P> 0.05) with treatments P0, P1, P3 and P4.
Effect of date palm (Phoenix dactylifera l.) on spermatozoa viability of kancra fish (Tor soro Valenciennes 1842) 48 hours post cryopreservation
Authors: Alifiani D P , Abinawanto  , Subagja J , Kristanto A H 
Published: February 2020

AbstractKancra is an endemic fish used in traditional functions in different parts of Indonesia. However, the population of the fish is decreasing due to environmental damage and excessive fishing. Cryopreservation of sperm of kancra fish is one of the most effective approaches to solving this problem. One of the success factors of cryopreservation is cryoprotectant. This study aims to evaluate the date palm juice as a natural cryoprotectant on the viability percentage of kancra fish after 48 hours of post cryopreservation. The sperm was collected by stripping and was diluted in the soluble liquid (fish ringer solution+methanol 10%+ date palm juice). The date palm juice concentration used were 0%, 5%, 10%, 15%, 20% and 25%. The sperm was equilibrated for 10 minutes at the 5°C temperature before it was frozen for 48 hours at the -10 °C. The thawing was done for 1 minute at 40 °C. Data were analyzed using ANOVA and followed by Tukey test. The result showed there was an effect of date palm juice (p<0.05) on the viability percentage of sperm post cryopreservation. The 10% concentration of date palm juice was optimum (p<0.05) with the percentage of viability 80.75 ± 1.19%.
The effect of fucoxanthin as coloring agent on the quality of Shrimp Paste
Authors: Zahrah Z , Amin M N G , Alamsjah M A 
Published: February 2020

Abstract
Colour is one of quality attributes of shrimp paste where some industries add non-food grade colouring agent, which may possibly generate adverse effects for customer. The development of colouring agent for shrimp paste is a crucial field of study in the food technology. Fucoxanthin, a yellow pigment extracted from Sargassum sp. which was proven capable of possessing antioxidant activity was used in this study to colorize the produced shrimp paste. The fucoxanthin concentration of 0%, 3%, 6%, 9%, and 12% was designed by using a completely randomized design with four replications of each level. This research revealed that the addition of fucoxanthin significantly affected the colour (L*, a*, b*, and °hue), but did not significantly affect the preference of odour and texture. This study investigated that the fucoxanthin concentration of 12% was the best concentration.
Hematological parameters of Catfish (Clarias sp) vaccinated by Aeromonas hydrophila with different application methods
Authors: Nadiro V N , Puspitasari I , Setyastuti T A , Santika A 
Published: February 2020

Abstract
The study aim was to find the effect of different vaccination application methods on hematological parameters of catfish (Clarias sp). A 107
Aeromonas hydrophila measured by 625 nm wave length of Spectrophotometer was applied on 25 catfish with average weight 15.83 g. The application methods were dipping (1 ml/10 l water) and intramuscular injection (0.1 ml/fish). Hematological parameter measured were hematocrit levels, total leukocytes and phagocytic index. Measurement were conduct on day 0, 21, 23 and 27 after vaccine application. The results showed that hematocrit level on fish with injection method were higher (21.02%) than dipping method (15,05%), total leukocytes on fish with dipping method were higher (3,29 x 104 cells/mm3) than injection method (3,15 x 104 cells/mm3) and phagocytic index on fish with injection were higher (25%) than dipping method (23%). The survival of catfish were also observed, and showed that catfish with injection method have higher survival rate (36%) than dipping method (34%). Aeromonas hydrophila vaccine applied with injection method have shown better immune response on catfish (Clarias sp) hematological parameters than one with dipping method.
Optimization of spermatozoa cryopreservation of Albino pangasius catfish: cryoprotectants with various concentrations and different equilibration times
Authors: Hasanah U , Abinawanto  , Alimuddin A , Boediono A 
Published: February 2020

Abstract
This study was aimed to optimize the spermatozoa cryopreservation protocol in Albino pangasius catfish by assessing the effectiveness of different cryoprotectant concentrations and different time of equilibration. The first experiment was conducted to optimize the combination of 10% methanol with different concentration of skim milk (0%, 5%, 10%, 15%, and 20%). The second experiment was aimed to optimize different equilibration time (10, 20, and 30 minutes). Deep freezer was used for the cryopreservation with a temperature of -80 °C at a storage period of 8 days. The first study found that the viability and motility of sperm after cryopreservation was significantly different (P<0.05) with fresh sperm. The best result was obtained at the 10% skim milk concentration with sperm viability of 78 ± 4.35% and motility of 69.66 ± 1.52%. The second experiment identified a significant different (P<0.05) in sperm viability and motility between fresh sperm and sperm post equilibration. The best result was obtained at 10 minutes equilibration time with sperm viability of 76 ± 1.15% and motility of 66 ± 2.64%. The study concluded that the 10% methanol with 10% skim milk, and 10 minutes equilibration time is the best combination for cryopreservation of Albino pangasius catfish spermatozoa.
The optimal n/p ratio of shrimp culture waste liquid fertilizer on growth of Chlorella vulgaris
Authors: Hidayati P A , Mubarak A S , Sudarno  
Published: February 2020

AbstractEnvironmental quality protection is done by processing shrimp waste into materials of high use value, including liquid fertilizer. The value of N/P ratio in shrimp culture waste fertilizer is still relatively low so it needs to add urea as a source of nitrogen to increase the N/P ratio in fertilizer. The purpose of this research was to determine the optimal N/P ratio in shrimp culture waste fertilizer to the growth ofChlorella vulgaris. The research method used in this research was an experimental method, A completely randomized design with 5 treatments and 4 replications. The treatments used are giving different N/P ratios from shrimp culture waste fertilizer enriched by urea, with N/P ratio 10:1, 15:1 and 20:1, whereas Walne fertilizer with N/P ratio 17:1 and shrimp culture waste fertilizer with N/P ratio 5,5:1 as a control. Data analyze was processed use ANAVA and followed by DMRT. The results of the research showed that the using of different N/P ratios in shrimp culture waste fertilizer enriched by urea gave a significantly different effect on the population and growth rate with the highest population average of 1030x104cell/ml and an average growth rate of 203x104cell/ml/day during the exponential phase.
Profile of Kerandang Fish (Channa pleurophthalmus Blkr) Proteins from Central Kalimantan
Authors: Aryani  , Suprayitno E , Sasmito B B , Hardoko  
Published: February 2020

AbstractKerandang fish (Channa pleurophthalmusBlkr) as one of freshwater fish from the Channidae family which is very potential and abundant in Central Kalimantan. The aim of this research was to determine and obtain a picture of the protein profile Kerandang fish. The benefit of this research is that Kerandang fish can be utilized maximally as one of the nutritious foods to demand the nutritional needs of the people in Central Kalimantan. This research was conducted exploratively qualitative with the parameters of the proximate test, amino acids using HPLC and determination of protein molecular weight using SDS-PAGE. The results obtained for the proximate test were 76.48% water content, 16.35% protein content, 0.35% fat content and 6.82% carbohydrate content. The results for amino acids assay obtained 15 types of amino acids, 8 types of essential amino acids with the highest type of amino acid, Leucine 4.69% and 7 types of non-essential amino acids with the highest type of amino acid, glutamic acid 9.11%, and total acid amino 53.32%. The result of analysis of protein profile of Kerandang fish meat based on its molecular weight using SDS-PAGE there are 27 protein bands in the range of molecular weights 10.202 - 134.432 kDa.
Change of hepatopancreas conditions in intensive shrimp aquaculture (Litopenaeus vannamei) at Mayangan Village, Legon Kulon District West Java
Authors: Dana D A , Setyastuti T A , Puspitasari I , Sukamto D 
Published: February 2020

Abstract
The study of hepatopancreas purpose to monitor changes in the health condition of shrimp, which is hepatopancreas is a vital organ of metabolism and digestive system. This study was conducted in intensive shrimp ponds in Mayangan Village, Legon Kulon for 82 days. Sampling was carried out every 10 days. Hepatopancreas preparation was conducted by taking 30-50 mg with adding 0.1-0.2 ml of immersion liquid and observing used a microscope at 40x magnification. The parameters of hepatopancreas observed such as microphili, lipids, wrinkles, and necrosis. After monitoring the microphili in the Day of Culture (DOC) 1 - 40, the average score range shows that the culture shrimp is 90-100% healthy. in DOC 43 until the last sampling has decreased by 40-0% which is assumed to be the effect of a White Feces Disease (WFD). The lipid and wrinkle in DOC 1-40 sampling showed that the average range shrimp culture was 90-100% healthy. While in DOC-43 sampling until the last of lipid and wrinkle sampling decreased by 60-0%. Hepatopancreas necrosis in DOC 1-31 sampling does not exceed from 20%, while DOC-40 sampling until the last of necrosis sampling continues to increase to 100% assumed by WFD and WSSV (White Spot Syndrome Virus).
The efficacy of probiotic with different storage to decrease the total organic matter, ammonia, and total Vibrio on shrimp pond water
Authors: Farizky H S , Satyantini W H , Nindarwi D D 
Published: February 2020

Abstract
Organic matter, ammonia, and total Vibrio are some parameters in shrimp culture media that can influence the success of shrimp culture. This study aims to determine the effect of Pseudomonas and Bacillus probiotic storage duration on decreasing organic matter, ammonia, and Total Vibrio in shrimp pond water. This study used a Completely Randomized Design (CRD) with five treatments namely without the addition of probiotics (P0, control), addition of fresh probiotics (P1), addition of probiotics with a storage period of 1 month (P2), addition of probiotics with a storage time of 2 months (P3) and addition of probiotics with a storage period of 3 months (P4). Each treatment repeated four times. The content of total organic matter and Vibrio in water measured on day 0 (initial), and third (end), ammonia content was measured every 24 hours during the study (t0-t3). The results showed that the addition of probiotics with a long storage different give in decreasing total organic matter, ammonia, and Vibrio in shrimp pond water. Addition of probiotics with a storage period of 3 months gave a Total Vibrio, organic matter, and ammonia content lower compared to the shrimp pond water without adding probiotics and gave significantly different (p <0.05).