bluuebunny/crossref_metadata_2025_split huggingface dataset:
The BEACON Study: Protocol for a cohort study as part of an evaluation of the effectiveness of smartphone-assisted problem-solving therapy in men who present with intentional self-harm to Emergency Departments in Ontario
Authors: Hatcher Simon , Heisel Marnin , Ayonrinde Oyedeji , Campbell Julie Kathleen , Colman Ian , Corsi Daniel J. , Edgar Nicole E. , Gillett Lindsay , Kennedy Sidney H. , Hunt Sophia Lakatoo , Links Paul , MacLean Sarah , Mehta Viraj , Mushquash Christopher , Raimundo Alicia , Rizvi Sakina J. , Saskin Refik , Schaffer Ayal , Sidahmed Alaaddin , Sinyor Mark , Soares Claudio , Testa Valerie , Thavorn Kednapa , Thiruganasambandamoorthy Venkatesh 
Published: May 2020

Abstract
Background: Patients who present to Emergency Departments (ED) after intentional self-harm are at an increased risk of dying by suicide. This applies particularly to men, who represent nearly two-thirds of those who die by suicide in Ontario. One way of potentially addressing this gap is to offer a course of blended problem-solving therapy (PST), comprised of a brief course of evidence-based psychotherapy for individuals at-risk for suicide, facilitated by the use of a patient-facing smartphone application and a clinician-facing “dashboard”. This approach has the potential to combine the benefits of face to face therapy and technology to create a novel intervention. Methods: This is a cohort study, nested within a larger pragmatic multicentre pre- and post-design cluster randomized trial (cRCT). Suicidal ideas assessed by the Beck Scale for Suicide Ideation (BSS) is the primary outcome variable. Secondary outcome measures include: depression (PHQ-9); anxiety (GAD-7); PTSD (PC-PTSD); health-related quality of life (EQ-5D-5L); meaning in life (EMIL); perceived social supports (MSPSS); alcohol use (AUDIT); drug use (DAST-10); problem-solving skills (SPSI-R:S); self-reported health care costs; and, health service use measured using Ontario administrative health data. A process evaluation will also be conducted following study completion. Discussion: The cohort study will test whether better adherence to the intervention results in better outcomes. The value of the cohort study design is that we can examine in more detail certain sub-groups or other variables that are not available in the larger cluster randomised trial. This trial will aim to improve standards by informing best practice in management of men who self-harm and present to hospitals in Ontario. Trial registration: ClinicalTrials.gov: NCT03473535, Registered on March 22, 2018, https://clinicaltrials.gov/ct2/show/NCT03473535
Exons sequencing identifies a novel mutation in GPR157 in a high myopia family
Authors: Tang Tao , Liu Mohan , Wei Ting , Deng Lin , Zhang Yueyang , Pu Yu , Cai Yan , Song Qiling , Zhang Ting , liu qingsong 
Published: May 2020

Abstract
Background Next-generation sequencing (NGS) and whole exome sequencing (WES) have identified many potential disease-causing loci and genetic mutations of high myopia(HM). However, these known genes can only explain the heritability of a small proportion of HM patients. A large proportion of variants have yet to be discovered. Herein we aimed to investigate the genetic characteristics of HM through a Chinese HM family(the inheritance pattern unknown) . Methods We performed WES on the parent-offspring trio and identified mutations by Sanger sequencing. All the members in this family were sequenced to validate phenotype co-segregated with candidate genes via Sanger sequencing as well. Besides, mutations detected were further evaluated in a cohort of 110 sporadic high myopia controls and 200 unrelated ethically-matched controls. And reverse transcription PCR(RT-PCR) was applied to measure the mRNA expression levels of GPR157 in the 4-week-old KM mice. Results A novel heterozygous nonsense mutation, c.859C>T (p.Arg287*) of GPR157 gene, was detected in the proband and her father by WES. And this disease-associated mutation was not found in 310 control individuals. For the family under study, HM was classified as autosomal dominant inheritance with reduced penetrance. And RT-PCR results showed GPR157 was abundantly expressed in the eye. Conclusion The hybrid nonsense mutation of the GPR157 gene identified in this study may constitute a novel genetic cause of HM. Keywords :high myopia, WES, GPR157
The role of perforating artery pedicled neurotrophic flap in the treatment of compound tissue defect of tibia with Ilizarov technique.
Authors: Wu Yuan-jian , Zhang Yu-bo , Zhang Wen-jun , Zhang Tao , Lou Tengfei , Han Pei 
Published: May 2020

Abstract
Background

The aim of this research is to present our experience with the combined use of pedicled neurotrophic flap and distraction osteogenesis in management of complex lower extremity injuries with composite bone and soft tissue defects and assess the functional and cosmetic results of this method.
Methods

Between 2008 and 2016, soft tissue coverage with pedicled neurotrophic flap followed by distraction osteogenesis using Ilizarov external fixator was performed in 25 patients for reconstruction of segmental bone defects of lower extremity complicated by massive loss of soft tissue. There were 16 men and 9 women: their mean age was 39 years (23 to 57)at the time of surgery. The soft tissue defect after radical debridement ranged from 5 × 9 cm2 to 11 × 14 cm2, and the average size of segmental defect was 5.02 (2.5 to 7.5) cm.
Results

The distally based sural neurovascular flap was performed in 13 patients, and the greater saphenous neurocutaneous perforator flap in 12 patients. All flaps survived completely without complications. The bone defects were corrected by a mean lengthening of 6.74 (4.5 to 9.5) cm. The function was judged to be excellent in 12 patients and good in 13 patients. Bone results were graded as excellent in 18 patients and good in 7 patients. Complications observed in the process of the treatment included pain, pin tract infections (PTI), ankle midfoot joints stiffness, and nonunion of docking sites. All the cases achieved successful limb salvage and satisfactory function recovery without recurrence of infection.
Conclusions

The combined technique of a perforator artery pedicled neurotrophic flap and distraction osteogenesis should be considered to be an effective alternative approach in the salvage treatment of the massively traumatized and chronically infected lower extremity.
Analysis of myocardial enzyme spectrum in 230 COVID-19 patients of Chongqing, China.
Authors: CaiLing Jiao , Fa Li Wen , YeHong Xie , JiaWei Miao , YunFang Wu , WanNing Tan , Jing Li , JianRong Zhang 
Published: May 2020

Abstract
Background

A novel coronavirus disease COVID-19 outbreak caused pandemic in China and worldwide. In addition to pneumonia, Cardiac failure is also a clinical outcome of coronavirus (COVID-19) patients and one of the leading causes for the death of COVID-19 patients. This study focused on a spectrum of cardiac enzymes to provide biomarkers for the severity of cardiomyopathy, and provide guidance of clinical treatment.
Methods

230 coronavirus patients (182 mild and 48 severe cases) enrolled in Three Gorges Hospital of Chongqing University from January to March 2020 were analyzed for a spectrum of cardiac injury enzymes including α-hydroxybutyric dehydrogenase (αHBDH), lactic acid dehydrogenase (LDH), creatine kinase (CK), and creatine kinase isoenzyme (CK-MB).
Results

The severe cases had significantly higher myocardial enzyme levels than mild cases, regardless of male and females. Males appeared to be more susceptible than females to COVID-19 induced heart injury, having higher CK and CK-MB in mild cases, and higher αHBDH and LDH levels in severe cases. Age is also a susceptible factor to COVID-19, but affected males were younger than females.
Conclusions

This study reveals that the heart is also a major target of COVID-19 infection, and myocardial enzyme spectrum assays could help the diagnosis, prognosis and guide the treatments to prevent heart failure in COVID-19 patients.
CCNI2 knockdown inhibits the proliferation, apoptosis and migration of pancreatic cancer cells
Authors: Hu Bingyang , Zhang Wenzhi , Zhang Changsheng , Li Chonghui , Zhang Ning , Pan Ke , Ge Xinlan , Wan Tao 
Published: May 2020

Abstract
Background: Pancreatic cancer has been recognized as one of the most serious malignant tumors in the world, and its molecular mechanism is still not fully understood. Cyclin I-like (CCNI2) is a homolog of Cyclin I (CCNI), and at present its function is largely unknown. Methods: In this study, we aimed to explore the role of CCNI2 in the development of pancreatic cancer. The expression levels of CCNI2 in pancreatic cancer tissues and cells were detected by immunohistochemical analysis and qPCR, respectively. Lentivirus was used to deliver shRNA to pancreatic cancer cells to construct CCNI2 knockdown cell models. MTT and colony formation assays were used to assess cell proliferation capacity, flow cytometry was used to detect apoptosis, wound-healing and Transwell assays were used to determine cell migration. Results: Our results revealed that CCNI2 is not only highly expressed in pancreatic cancer, but also significantly correlated with pathological grade, pathological stage, and survival rate. It was confirmed that knockdown of CCNI2 inhibited the proliferation and cell migration of pancreatic cancer cells while promoting apoptosis. Furthermore, human apoptotic antibody arrays showed that CCNI2 is involved in apoptosis process by up-regulating the pro-apoptotic proteins. Conclusions: In conclusion, CCNI2 may be a prognostic marker for pancreatic cancer and is associated with its development. Thus, CCNI2 possesses potential to act as a therapeutic target for pancreatic cancer treatment.
Day surgery scheduling and optimization in large public hospitals in China: a three-station job shop scheduling problem
Authors: Bai Xue , Zhang Wei , Luo Li , Ma Hong-Sheng , Zhu Ting 
Published: May 2020

Abstract
Background

Efficiency and flexibility constitute the prime performance index that is used to evaluate the Day Surgery Center (DSC). Based on the day surgery service process for large public hospitals in China, we found that the service efficiency of the overall process depends on the utilization of day operating rooms (ORs and could be optimized through key resource management and scheduling. Day surgery scheduling can be described as being a flexible flow shop owing to the three-station nature of surgery. Allocating all types of hospital resources to the three stations and determining the length of time for each stage during surgeries is crucial to improve the efficiency of a hospital’s DSC.
Methods

This paper integrates a three-station job shop scheduling problem (JSSP) into the day surgery scheduling and optimization problem. The JSSP was formulated as a mixed-integer linear programming model, and the elicitation of the model for scheduling surgeries with different priorities in the DSC is discussed. The model illustrated a detailed example of the DSC within West China Hospital. Numerical experiments based on the genetic algorithm design were conducted.
Results

We found that the optimal scheduling strategy could not only minimize the makespan of the whole day surgery process, which would improve the efficiency of the DSC in large public hospitals in China, but also allow for timely scheduling adjustments during the advent of emergency surgeries, which is essential for the efficient management of day surgery rooms in large public hospitals in China.
Conclusions

By further refining the day surgery procedure, the JSSP model dispatches the resources involved in the various processes of the surgery. The switching time is reduced owing to the subdivision of the surgery process, resulting in an increase in OR utilization, promoting the standardization of the surgery process. We found that the JSSP model is more suitable for the efficient management of day surgery rooms of large public hospitals in China. Although this study considered a variety of resource constraints based on the day surgery service process implemented in China, there are still some shortcomings which needs further research.
Metformin Inhibit Cell Proliferation and Secretion Function in NCI-H295R Cells
Authors: Li Chunyan , Wang Fen , Tong Anli , Yan Zhaoli , Li Yuxiu 
Published: May 2020

Abstract
Background: The evidence about effects of metformin on adrenocortical carcinoma cell lines is lacking. This study aims to investigate the effects of metformin on proliferation and secretion function of H295R cells.Methods: H295R cells were treated with different doses of metformin for 3 days or with 20 mmol/L metformin for different times. Cell proliferation was detected by MTS method. Cortisol and aldosterone in culture medium was determined by chemiluminescent method and radioimmunoassay, respectively. H295R cells were treated with 20 mmol/L metformin for 24 hours, and mRNA expressions of 11 beta-hydroxylase (CYP11B1) and aldosterone synthase (CYP11B2) were detected by real-time quantitative PCR.Results: The results showed that no differences of cell proliferation and secretion of cortisol and aldosterone were detected between control group and metformin group with doses less than 5 mmol/L. Metformin with large doses (≥ 10 mmol/L) significantly inhibited cell proliferation and secretion of cortisol and aldosterone in a dose-dependent pattern. Metformin (20 mmol/L) inhibited cell proliferation after 12 hours’ incubation. And the proliferation inhibitory effects of metformin were in a time-dependent manner. Compared with control group, metformin decreased secretion of cortisol and aldosterone, and mRNA expression of CYP11B1.Conclusions: Metformin can inhibit cell proliferation and secretion of cortisol and aldosterone in H295R cells.
MiR-450 inhibits cell proliferation, migration and invasion and induces apoptosis in gastric cancer via targeting CREB1
Authors: Zhao Ya-Jun , Zhang Jun , Wang Yong-Cang , Wang Liang , He Xin-Yang 
Published: May 2020

Abstract
Background：Gastric cancer seriously affects human health and research on gastric cancer is increasing. In recent years, molecular targets are hot research topics. We aimed to explore the effects and mechanisms of miR-450 on the development and progression of gastric cancer.Methods：We used gain-of-function approaches to investigate the gastric cancer cell proliferation, apoptosis, migration and invasion, including, RT-qPCR, CCK-8, colony formation, flow cytometry, western blot, wound healing, transwell chamber, HE, TUNEL, dual luciferase reporter and tumor formation analysis.Results：We found that the expression levels of miR-450 were greatly decreased in gastric cancer cells and overexpression of miR-450 inhibited the gastric cancer cell proliferation, migration and invasion, while induced apoptosis in gastric cancer in vitro. Moreover, we demonstrated that ectopic expression of miR-450 inhibited tumor growth in vivo. At the molecular level, overexpression of miR-450 significantly increased the expression levels of apoptosis-associated proteins, including Caspase-3, Caspase-9 and BAX, while inhibited the expression level of Bcl-2. Mechanically, luciferase reporter experiment suggested that CREB1 had a negative correlation with miR-450 expression and knockdown of CREB1 alleviated gastric cancer. Furthermore, we also found that miR-450 inhibited the activation of AKT/GSK-3β signaling pathway to inhibit the progression of gastric cancer.Conclusions：miR-450 repressed gastric cancer cell proliferation, migration and invasion and induced apoptosis through targeting CREB1 by modulating AKT/GSK-3β signaling pathway, which may provide a new molecular target for the treatment of gastric cancer.
Prevalence high risk Human Papillomavirus (HPV) in HIV-infected women from Belém, Pará, Amazon region of Brazil: A cross-sectional study
Authors: Monteiro Jacqueline , Fonseca Ricardo Roberto de Souza , Ferreira Tuane , Rodrigues Luana , Silva Andreza , Avelino Maria Eduarda , Menezes Cláudia , Gomes Samara , Silvestre Rodrigo , Silva Andréa da , Pamplona Ilze , Vallinoto Antônio , Ishak Ricardo , Machado Luiz Fernando 
Published: May 2020

Abstract
Background: Human papillomavirus (HPV) is the most common sexually transmitted disease in the world. Several studies have shown a higher prevalence of HPV infection in HIV-infected women. The aim of this study was to determine the prevalence and the genotype diversity of HPV infection in HIV-infected women.Methods: From April 2010 to December 2012 cervical specimens were collected from 169 HIV-infected women who screening for cervical cancer at Reference Unit in Belém. The detection of HPV infection was performed by nested PCR and HPV type was performed using the commercial kit.Results: The prevalence of HPV infection was 63,3%. Of the 47 genotyped samples, 40,4% was found positive for high risk-HPV 16 and 12.8% for high risk-HPV 52. HPV infection was predominant in the group of women with no incidence of cytological abnormalities and more prevalent in women of reproductive age, unmarried, low education level and who used condoms during sexual intercourse. It was observed an association between HPV infection and independent variables, such as condom use, multiple sexual partners and history of sexually transmitted diseases.Conclusions: High-risk types of HPV infection were prevalent in our study. Infection with multiple high-risk HPV genotypes may potentiate the development of cervical cancer in HIV-infected women.
Determinants of Adherence to Dental Treatment of Adolescents in Social Vulnerability in Primary Care
Authors: Bulgareli Jaqueline Vilela , Cortellazzi Karine Laura Cortellazzi , Guerra Luciane Miranda , Ambrosano Gláucia Maria Bovi , Kaieda Armando Koichiro , Cunha Inara , Vazquez Fabiana Lima , Pereira Antonio Carlos 
Published: May 2020

Abstract
Background Different studies with adolescents refers to the difficulty they have to adhere to oral dental treatments. Therefore, better understanding the processes involved in adherence to treatment in this population is necessary. The aim of this study was to investigate the factors that influence adherence to dental treatment in social underprivileged adolescents in primary care.Methods A longitudinal analytical study was conducted in Piracicaba, São Paulo, Brazil, between 2014 and 2015.The sample consisted of 1179 adolescents whom were examined in family health units, these 474 were referred for treatment (40,2%) and these 325 (68,6%) teenagers were reevaluated after 18 months. Adherence to dental treatment was the dependent variable. Independent variables were: individual (clinical, socio demographic, access to the service, reporting pain, oral impacts on daily perfomance, family cohesion) and the contextual (percentage of families in the neighborhood with income of 0.5 to 1 minimum wage).Results Non-adherence rate to treatment was high in the studied sample (49.5%).Family income (p = 0.039) and household crowding (p = 0.003) were associated with non-adherence to dental treatment.Conclusions It is concluded that the condition of social vulnerability of adolescents resulted in competing situations with adherence, a fact that makes it difficult dental treatment and action planning health services.
Eosinophil clusters in patients with chronic obstructive pulmonary disease (COPD): a multicenter study
Authors: Zucchi José William , Franco Estefânia Aparecida Thomé , Schreck Thomas , Silva Maria Helena Castro e , Migliorini Sandro Rogerio dos Santos , Garcia Thaís , Mota Gustavo Augusto Ferreira , Morais Bruna Evelyn Bueno de , Machado Luiz Henrique Soares , Batista Ana Natália Ribeiro , Paiva Sergio Alberto Rupp de , Godoy Irma de , Tanni Suzana Erico 
Published: May 2020

Abstract
The authors have removed this preprint from Research Square.
Abdominal obesity phenotypes outcomes: protocol for a systematic review
Authors: doustmohamdian soraya , hosseinpanah farhad , momeni marjan 
Published: May 2020

Abstract
Background: The prevalence of obesity is increasing worldwide. Obesity is associated with severe health effects. Abdominal obesity has a strong association with metabolic dysfunction, diabetes mellitus type 2 (DM2), dyslipidemia, hypertension, and coronary artery events. Methods: We will search PubMed/MEDLINE, EMBASE, Web of Science, Cochrane Library, and ProQuest (from inception onwards). Additional studies will be identified through manual searching of reference lists. Quantitative studies evaluating abdominal obesity phenotype outcomes in adults will be included. Primary results will be assaying abdominal obesity phenotype results, including DM2 incidence, cardiovascular disease risk, and all-cause mortality. Two reviewers will independently screen full-text articles and abstract data. Potential conflicts will be resolved through discussion. The study methodological quality (or bias) will be appraised using appropriate tools. If feasible, we will conduct a random-effects meta-analysis. The two researchers will also assess the quality of the articles independently based on CASP. Discussion: This systematic review will summarize evidence regarding the association between abdominal obesity phenotypes with DM2,cardiovascular disease and all cause mortality.The results of this review will provide a useful reference for the importance of abdominal obesity on metabolic dysfunction and mortality
Effects of Iron Therapy on the reduction of anemia among women of reproductive age in developing countries: A protocol for systematic review and Meta-analysis
Authors: Ali Sumera Aziz , Aziz Savera , Allana Ahreen , Ali Arzina Aziz , Raheman Fazal Ur- 
Published: May 2020

Abstract
Background Iron deficiency anemia is a major public health issue among women of reproductive age (WRA), as it leads to high maternal and infant morbidity and mortality. The WHO recommends the distribution of iron supplements to all women in populations in which the prevalence of anemia (in women) exceeds 20%. Interventional studies have been carried out in developing countries regarding the efficacy and safety of iron in reducing anemia among women of reproductive age. However, the findings from randomized controlled trials have not been systematically synthesized. Therefore, we performed a systematic review and meta-analysis of RCTs to both qualitatively and quantitatively assess the evidence for the efficacy and effectiveness of iron as an intervention for reducing anemia among women of reproductive age in developing countries. Methods This review and meta-analysis will focus on assessing the efficacy and effectiveness of iron as an intervention for reducing anemia among women of reproductive age. PubMed, Google Scholar, and Science Direct will be explored using a detailed search strategy. A study will be considered eligible if it is a randomized controlled trial determining the efficacy and effectiveness of iron in anemia reduction among WRA (15-49 years) in developing countries and published in the English language in a peer-reviewed local or international journal from 2000 to 2020. We will perform a meta-analysis to measure the effect of individual studies as well as the pooled effect of all studies by generating forest plots. Heterogeneity will be assessed using I 2 statistics and a funnel plot will be developed to assess the publication bias. Discussion This systematic review will provide an opportunity to synthesize and critically appraise the findings from individual studies on the efficacy and effectiveness of iron as an intervention for reducing anemia among women of reproductive age to generate a comprehensive summary. These aggregated findings, in turn, can guide clinical practice and help clinicians, researchers and policymakers make evidence-based decisions to address this pressing health concern among women of reproductive age residing in developing countries. The findings of the review will be disseminated through presentations and peer-reviewed publications.