bluuebunny/crossref_metadata_2025_split huggingface dataset:
Perioperative worries associated with low acceptance of day surgery among outpatients in Zhejiang Province, China: a survey research
Authors: Feng Tonghui , Li Yujia , Zhou Daipeng , Xu Peng , Lu Xing , Xia Yanfei , Wang Hongmei 
Published: October 2019

Abstract
Background:Day surgery, a popular medical practice in the United States, started rather late in mainland China since 2001 when day surgery was officially launched. Determining Chinese patients’ specialty and attitudes towards day surgery is important for proactive patient education to promote day surgery in China. At present, Chinese patients’ attitudes towards day surgery are blurred with only few relevent research.The goal of this study was to investigate Chinese patients’ specialty, especiallyperioperative worries,and its relationship with patients’ attitudestowardsday surgery in Zhejiang Province, China.
Methods: A total of 412 outpatients in Zhejiang Hospital were evaluated by a cross-sectional questionnaire survey between January 2019 andFebruary 2019.To evaluate outpatients’ specialtyand attitudes towardsday surgery, 420 outpatients were asked to fill out questionnaires including their characteristics, perioperative worry and acceptance of day surgery. Multinomial Logistic regression model was used to assess the relationship between them.
Results:Of the 412 outpatients, 83.5% reported perioperative worries about day surgery. Outpatients who were older, less-educated, with comorbidities and lower income were more worried perioperatively and had poorer acceptance of day surgery. Outpatients worriedmost aboutpreoperative examinations not being done timely and being unable to monitor and manage complications and adverse events. Age, educated degree, comorbidities, financial status, surgery type and perioperative worry hadsignificantassociations with patient acceptance of day surgery.Outpatients with worries significantly scored lowerin acceptance of day surgery than those without worries, thus had a higher risk of refusing day surgery. Compared with less-educated patients, perioperative worries played a greater role in decreasing patient reception of day surgery in better-educated patients, reducing the possibility of acceptingday surgery.
Conclusions:Perioperative worry was identified as an independent factor in influencing patient acceptance of day surgery among outpatients in ZheJiang Province in China.Results of this study can be used to guide patient education according to patients’ characteristics in day surgery, helping promote day surgery in China.
Study on genetic polymorphisms and phenotypic frequency distribution of CYP2C9, CYP2C19 and CYP2D6 in Han Chinese population
Authors: He Li , Chen Shaojun , Li Jingao , Xie Xiaoxue , Huang Lihua , Kuang Yun , Xu Kangwei , Huang Wanxia , Zhao Yanling , Yang Guoping , Guo Chengxian 
Published: October 2019

Abstract
Purpose Genetic mutations and phenotypic changes of CYP2C9, CYP2C19 and CYP2D6 are vital for individual variations in clinical drug responses. Elucidating the mutating frequencies and phenotypic distributions of these genes shall facilitate the implementation of preemptive pharmacogenetic testing. We analyzed the gene polymorphisms and phenotypic frequencies of CYP2C9, CYP2C19 and CYP2D6 in Han Chinese population. Methods Tests of CYP2C9, CYP2C19 and CYP2D6 were performed in over 3200 (3276-3293) samples in Han Chinese population; furthermore, systematic review was employed for analyzing the mutation frequency and phenotypic distribution of CYP2C9 and CYP2C19 in Han Chinese population. Results Among 9062 samples, the mutation frequency of CYP2C9 was 11.49% and the frequency of phenotypic changes 7.1%; in 8222 samples, the mutation frequency of CYP2C19 was 66.07% and the frequency of phenotypic changes 63.75%; among 3931 samples, the mutation frequency of CYP2D6 was 88.04% and the frequency of phenotypic changes 3.87%. Among 2690 samples, gene mutations and phenotypic distributions of CYP2C9, CYP2C19 and CYP2D6 were simultaneously examined and it was found that 96.36% samples became mutated and the frequency of phenotypic changes was 66.51%. Conclusions In Han Chinese population, the gene mutations and phenotypic changes of CYP2C9, CYP2C19 and CYP2D6 are all relatively frequent. Prior to dosing, preemptive pharmacogenetic testing of CYP2C9, CYP2C19 and CYP2D6 is recommended.
Analysis of serum CircRNA in related to gastric cancer
Authors: Chen Gang , Su Yun , Gong HONG-XIA , Li Ling , Zhang Zhi-Yi , Wang Feng-Mei , Nie Peng , Ma Guo-Gang , Wang Xin-Hua , Li Chun-Bo , Xue Xuan , Li Ting-Ting , Liu Yong-Qi 
Published: October 2019

Abstract
Gastric cancer is one of the most common malignant tumors of the digestive tract and one of the leading causes of death in patients with malignant tumors worldwide. In recent years, with the deepening of circRNA research, more and more evidence indicates that circRNA plays an important role in the occurrence and development of human malignant tumors. This study firstly conducted a retrospective analysis of the case data of gastric cancer patients who were treated at the Wuwei Cancer Hospital between 2015 and 2017. Subsequently, 18 cases of primary gastric cancer patients and 9 healthy people in Wuwei, Gansu Province were used as controls. The high-throughput microarray technology was used to screen the circRNA expression profiles of healthy and gastric cancer patients, and the expression was expressed by bioinformatics methods. Differential circRNA was used for gene ontology (GO) and KEGG pathway analysis, using its enrichment to predict the relevant biological functions of the differentially expressed circRNA and its involved pathways, and predicting miRNAs interacting with differentially expressed circRNAs, and constructing circRNA-miRNA interaction network. Q-PCR, gene organization microarray and bioinformatics techniques were used to validate candidate differential circRNAs and their linear parental genes and regulatable miRNAs. The results showed that there were 137 circRNAs with significant expression differences (including up-regulation of 67 and down-regulation of 70) in gastric cancer patients, and their differential expression may be related to the occurrence and development of gastric cancer; by GO, KEGG enrichment analysis and Regulating miRNA predictive analysis, the gastric cancer-related GO classification, KEGG Pathway and circRNA-miRNA network were preliminarily obtained, suggesting that differential circRNA may participate in gastric cancer-associated GO classification and KEGG pathway by regulating the expression of parental genes and miRNAs to influence the occurrence and development of gastric cancer. Finally, by using has verified the has_circ_0000437 and its parental genes and regulatable miRNAs, it was found that has_circ_0000437 is highly expressed in gastric cancer patients, and has a certain diagnostic value for the clinical diagnosis of gastric cancer. It may regulate its linear parental gene and The expression of miRNAs affects the development, metastasis and prognosis of clinical gastric cancer.
An Epidemiology of Self-Reported Cancer among women in India: A Decomposition Analysis
Authors: Singh S.K. , Biswas Ayantika , Puri Parul 
Published: October 2019

Abstract
Background Cancer is a leading cause of adult deaths globally. It has a death toll of 7,84,821 people from India in 2018 alone. Huge underlying regional and sectoral (urban-rural) variations, extending to lifestyles and variations in age-specific death rates compels assessment of the geographical and social distribution is essential to frame cancer control programmes.Methods National Family Health Survey (2015-16) data (also called the Demographic Health Survey of India) has been used for this study. Results The results of the concentration indices show an overall concentration of cancer towards the richer quintiles. For the socially deprived groups, it is concentrated among the richer quintiles, while for the socially non-deprived groups, it is concentrated among the poorer quintiles. The P80/P20 ratio for cancer incidence is 0.83. The decomposition of the concentration index shows a significant contribution from women hailing from the socially deprived groups of the population.Conclusion A comprehensive strategy to combat chronic diseases like cancer includes actions minimizing exposure to risk factors at the population level, as well as reducing the risk for individuals at the higher risk level and can aid in furnishing early, medium-term and long-term effects.
Potential biomarkers of ductal carcinoma in situ progression
Authors: Dettogni Raquel Spinassé , Stur Elaine , Laus Ana Carolina , Vieira René Aloísio da Costa , Marques Márcia Maria Chiquitelli , Santana Iara Viana Vidigal , Pulido José Zago , Ribeiro Laura Fregonassi , Parmanhani Narelle de Jesus , Agostini Lidiane Pignaton , Reis Raquel Silva dos , Santos Eldamária de Vargas Wolfgramm , Alves Lyvia Neves Rebello , Garcia Fernanda Mariano , Santos Jéssica Aflávio , Ventorim Diego do Prado , Reis Rui Manuel , Louro Iúri Drumond 
Published: October 2019

Abstract
Background Ductal carcinoma in situ is a non-obligate precursor of invasive breast carcinoma and presents a potential risk of over or undertreatment. Finding molecular biomarkers of disease progression could allow for more adequate patient’s treatment. Our aim was to identify potentially biomarkers that can predict invasiveness risk.Methods In this epithelial cell-based study archival formalin-fixed paraffin-embedded blocks from six patients diagnosed with invasive lesions (pure invasive ductal carcinoma), six with in-situ lesions (pure ductal carcinoma in situ) , six with synchronous lesions (invasive ductal carcinoma with an in-situ component) and three non-neoplastic breast epithelium tissues were analyzed by gene expression profiling of 770 genes, using the nCounter® PanCancer Pathways panel of NanoString Technologies.Results The results showed that in comparison with non-neoplastic tissue the pure ductal carcinoma in situ was one with the most altered gene expression profile. Comparing pure ductal carcinoma in situ and in-situ component six differentially expressed genes were found, three of them ( FGF2 , GAS1 and SFRP1 ), play a role in cell invasiveness. Importantly, these genes were also differentially expressed between invasive and noninvasive groups and were negatively regulated in later stages of carcinogenesis.Conclusions We propose these three genes ( FGF2 , GAS1 and SFRP1 ) as potential biomarkers of ductal carcinoma in situ progression, suggesting that their downregulation may be involved in the transition of stationary to migrating invasive epithelial cells.
The hospice as a learning environment: A follow-up study of a death education intervention that involved high-school students and the community
Authors: Testoni Ines , Sblano Fabio Vito , Palazzo Lorenza , Pompele Sara , Wieser Michael Alexander 
Published: October 2019

Abstract
Background

The theme of death in Western society has been removed from everyday life and replaced with specialized medical language. Such censorship does not reduce the fear of death, especially among young people, and it limits the possibility to elaborate on experiences linked to death, generating negative effects, especially in cases of traumatic grief. The objective of this follow-up study was to detect how and if experiences of death education changed the relationship between the community and a local hospice in relation to the theme of death and to the elaboration and management of suicide.

Methods

This was a qualitative research study based on semi-structured interviews with palliative care professionals and teachers of students who had taken part in a death education intervention 3 years earlier. The interviews were examined through thematic analysis.

Results

The results confirmed the efficacy of the death education intervention initiated 3 years earlier. The education initiative contributed to modifying the students’ and teachers’ perspectives of end-of-life professionals and their understanding of the structures within which palliative care is implemented. It also modified the participants’ perspectives on the representation of death and promoted a new outlook on life. The hospice staff also perceived a change in the attitudes of the community, and this change had a positive impact on their everyday work.

Conclusions

This study confirmed the usefulness of creating continuity between a hospice and the community through a school education initiative. The death education project provided an educational space in which it was possible to elaborate on experiences linked to death and to re-evaluate and appreciate the structures of end-of-life care and the professionals who work in the area.

Keywords: Hospice, Palliative care, Death education, Adolescents, Suicide.
Study on reproductive hormone disorder causing N-glycan structure change by swainsonine poisoning
Authors: Zhou Hongchao , Wang Yi , Zhao Baoyu , Wu Chenchen 
Published: October 2019

Abstract
Background: Glycosylation and glycan composition play an important role in the biological properties of follicle-stimulating hormone (FSH) and luteinizing hormone (LH). Swainsonine (SW) causes oligosaccharide accumulation and incomplete glycoprotein processing. Previous research has shown that SW reduces serum progesterone concentrations in pregnant livestock. However, we do not know how SW affects the secretion of reproductive hormones.
Materials: We used primary culture of mouse endometrial epithelial cells, Real-time polymerase chain reaction, Western blot, Periodic acid-Schiff (PAS) staining, Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF-MS), ELLSA, etc. method.
Results: In this study, we observed a significant decrease in the number of surviving fetal mice in the SW treatment group mice and a high level of carbohydrate deposition in PAS stained uterine tissue in the SW treatment group mice. Extensive vacuole degeneration can also be observed in mouse primary endometrial epithelial cell cultures in vitro after SW poisoning. MALDI-TOF-MS shows that the time of exposure to SW is prolonged, the number of di-, tri-, and tetra-complex glycosylated gradually decreases, and the number of tri- hybrid type glycosylated increases, while significantly reduced activity of glycosyltransferases and glycosidases are observed in endometrial epithelial cells. FSH and LH were significantly decreased at 7 and 15 days after pregnancy and 7 days after childbirth, the content of E2 and P4 decreased significantly at 7 days after childbirth, and the expression levels of mRNA and protein of 3β-hydroxysteroid dehydrogenase (HSD-3β) and cytochrome P450 family 19 subfamily A member 1 (CYP19A1) also showed a significant decrease at 15 days after pregnancy and 7 days after childbirth.
Conclusions: In summary, The glycan chain structure of FSH and LH glycoprotein hormones were changed by swainsonine, which they are decreased on the expression of the limiting enzyme of steroid hormones indirectly, and ultimately lead to a decrease in the content of E2 and P4. The rate of female mice were decreased by dysfunction of the reproductive hormones.
Non-alcoholic fatty liver disease, cytokeratin-18, and risk of type 2 diabetes mellitus: A cohort study
Authors: Hu Ruofan , Xu Shaoyong , Shen Han , Jing Ce , Jia Aihua , Yu Xinwen , Xing Ying , Liang Shengru , Ming Jie , Ji Qiuhe 
Published: October 2019

Abstract
Background & Aims: Although many studies have shown that non-alcoholic fatty liver disease (NAFLD) is associated with type 2 diabetes mellitus (T2DM), no cohort study has explored the relationship between the histopathological grade of NAFLD and the risk of T2DM in NAFLD patients. We aimed to explore whether a higher concentration of cytokeratin-18 (CK-18), as a reliable marker of hepatic fibrosis, was associated with a greater risk of T2DM in patients with NAFLD. Methods: The population-based cohort study was based on China National Diabetes and Metabolic Disorders Survey with a follow-up of five years. NAFLD was determined by ultrasonography. T2DM were diagnosed based on oral glucose tolerance test. Serum CK-8 was measured using the M30 Apoptosense ELISA kit. Results: 457 subjects were enrolled and three groups were analyzed: a non-NAFLD group (n=363), a low-CK-18 NAFLD group (n=46), and a high-CK-18 NAFLD group (n=48). 20 (3.9%) developed diabetes during follow-up. The incidence of T2DM was 2.5%, 8.7%, and 12.5% in the non-NAFLD, low-CK-18 NAFLD, and high-CK-18 NAFLD groups, respectively. Cox proportional hazard regression showed that, compared with the non-NAFLD group, the adjusted relative risks of T2DM were 3.37 (95% CI: 1.05-10.86, P =0.042) and 4.71 (95% CI: 1.71-12.99, P =0.003), respectively, in the low-CK-18 NAFLD and high-CK-18 NAFLD groups. Conclusions: Higher CK-18 level in ultrasound-diagnosed NAFLD patients is associated with higher risk of T2DM. We recommend screening for NAFLD using ultrasound in the first instance, with, if possible, CK-18 assay being subsequently used to screen individuals at higher risk of diabetes.
The Association of Abnormal Liver Enzymes with All-Cause and Cause-Specific Mortality: A Large Prospective Population-Based Study
Authors: Kolahdoozan Shadi , Motamed-Gorji Nazgol , Sharafkhah Maryam , Sepanlou Sadaf G. , Afshar Pedram , Malekzadeh Reza , Poustchi Hossein , Merat Shahin 
Published: October 2019

Abstract
Background: While the association of elevated levels of liver enzymes (ALT, AST, ALP and GGT) with mortality has been suggested in many studies, evidence of their association appears to be contradictory. The current study aims to investigate the associations of entire ranges of serum liver enzymes with mortality in a large cohort of adults. Methods: In the current study we used the data of repeated measurement phase in Golestan Cohort Study, which was conducted from 2010 to 2012. All liver enzyme levels were categorized into their quintile values, and the association of liver enzymes with mortality rates were evaluated using Cox proportional hazard regression models. Sensitivity analyses were performed by excluding data of first follow-up year, and patients with history of cardiovascular disease (CVD), cancer or viral hepatitis. Results: We included 11,106 individuals with mean age of 56.22 (±7.97) years, 46.2% male. Median follow-up period was 7 years. In the total population, ALT demonstrated a U-shaped association with all-cause mortality; whereas AST showed no significant association. ALP and GGT had linear and positive associations with overall mortality in the total population. CVD-mortality showed associations with low ALT (aHR=1.53, 95% CI: 1.1-2.2), high ALP (aHR=1.79, 1.3-2.6) and high GGT (aHR=2.0, 1.4-2.9). Cancer-mortality was associated with high ALP (aHR=1.9, 1.2-2.9, while GGT had a U-shaped association with cancer mortality, with the middle category (Q3) associated with the lowest cancer-mortality risk (aHR=0.58, 0.4-0.9). High ALT and high AST were associated with cancer-mortality in total sample as well; although, these associations disappeared after the sensitivity analysis. Conclusion: Both low and high levels of ALT are associated with increased all-cause mortality, while only high levels of ALP and GGT have positive associations with all-cause mortality. High levels of all four liver enzymes are associated with cancer mortality. However, only the high levels of ALP and GGT are associated with CVD mortality. Evidence implies that abnormal liver enzymes may be manifestations of metabolic syndrome, CVD, and cancers rather than being their precursors. Yet, they may be useful markers for predicting the survival of patients.
Comparing effectiveness of Metoclopramide alone versus its combination with Dexamethasone for prevention of postoperative nausea and vomiting after thyroidectomy at Menelik II Hospital, Addis Ababa, Ethiopia: a prospective cohort study
Authors: Tilahun Tamiru , Aregawi Adugna , Gelgelu Temesgen Bati 
Published: October 2019

Abstract
Background Postoperative nausea and vomiting (PONV) is a common post- operative unpleasant experience and also common following thyroidectomy that may cause for hematoma and post-operative airway obstruction. Several agents have been tried to reduce severity and incidence PONV.This study intended to compare effectiveness of Metoclopramide alone and its combination with Dexamethasone for prevention of PONV after thyroidectomy at Menelik II Hospital.Methods Prospective cohort study was conducted on 76 American Society of Anesthesiologist (ASA) class I and II, adult patients undergone thyroidectomy. A systematic random sampling was applied to identify sample population through skip interval till required sample size was achieved. Study participants were grouped as Group = M (Metoclopramide alone) and Group = MD (Metoclopramide plus Dexamethasone).Following study participant received either 10 mg Metoclopramide or 5mg Metoclopramide plus 4mg Dexamethasone, PONV was recorded at 6, 12 and 24 hours post operatively. Continuous variables were expressed as mean ± SD or median-IQR as appropriate. Analysis was done by independent two sample t test, Manny Whitney U test and χ2 or fisher exact test. P-Value <0.05 was considered as statistically significant.Results The overall incidence of PONV with in the first 24 hours was significantly higher in Group M as compared to Group MD, 24(63.15%) versus 9 (23.68%) respectively with a P-value of < .01. Although difference was insignificant, severity of nausea at 6th, 12th and 24th was still higher in Group M. From side effects, Sedation was significantly lower in Group MD 6 (15.79%) versus 15 (39.47%) with a P-value of <.05. Despite difference was insignificant headache was found higher in Group M 10 (26.31) vs. 6 (10.52) respectively.Conclusions 5mg Metoclopramide combined with 4mg Dexamethasone has significant effects for prophylaxis of post-operative nausea and vomiting after thyroidectomy. Some side effects of drugs were also significantly reduced in combination group.