bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association of CSF Proteins With Tau and Amyloid β&nbsp;Levels in Asymptomatic 70-year-olds&nbsp;
Authors: Remnestål Julia , Bergström Sofia , Olofsson Jennie , Sjöstedt Evelina , Uhlén Mathias , Blennow Kaj , Zetterberg Henrik , Zettergren Anna , Kern Silke , Skoog Ingmar , Nilsson Peter , Månberg Anna 
Published: December 2020

Abstract
BackgroundIncreased knowledge of the evolution of molecular changes in neurodegenerative disorders such as Alzheimer’s disease (AD) is important for the understanding of disease pathophysiology and also crucial to be able to identify and validate disease biomarkers. While several biological changes that occur early in the disease development have already been recognised, the need for further characterization of the pathophysiological mechanisms behind AD still remains.MethodsIn this study, we investigated cerebrospinal fluid (CSF) levels of 104 proteins in 307 asymptomatic 70-year-olds from the H70 Gothenburg Birth Cohort Studies using a multiplexed antibody-based technology. ResultsThe protein levels were first correlated with the core AD CSF biomarker concentrations of total tau, phospho-tau and Aβ42 in all individuals. Sixty-three proteins showed significant correlations with either total tau, phospho-tau or Aβ42. Thereafter, individuals were divided based on CSF Aβ42/Aβ40 ratio and Clinical Dementia Rating (CDR) score to determine if early changes in pathology and cognition had an effect on the correlations. We compared the associations of the analysed proteins to CSF markers between groups and found 33 proteins displaying significantly different associations for amyloid-positive individuals and amyloid negative individuals, as defined by the CSF Aβ42/Aβ40 ratio. No differences in the associations could be seen for individuals divided by CDR score.ConclusionsWe identified a series of transmembrane proteins, proteins associated to or anchored to the plasma membrane, and proteins involved in or connected to synaptic vesicle transport that were associated with CSF biomarkers of amyloid and tau pathology in AD. Further studies are needed to explore their role in AD pathophysiology.
Factors Associated with Risk Behaviors Towards Hepatitis B Among Migrant Workers: A Cross-sectional Study Based on Theory of Planned Behavior
Authors: Xiang Hui , Li Mingjing , Xiao Meng , Liu Min , Su Xiaoshan , Wang Dashu , Li Ke , Chen Rui , Gan Lin , Chu Kun , Tian Yu , Tang Xiaojun , Lei Xun 
Published: December 2020

Abstract
Background: Rural-to-urban migrant workers are susceptible to hepatitis B (HB) because they lack self-protection awareness and social support. The present study aimed to investigate the current status of risk behaviors (RB) regarding HB among migrant workers and the influencing factors defined by the Theory of Planned Behavior (TPB).Methods: A cross-sectional study was performed by two-stage cluster sampling from June to December 2018 in Chongqing, China. Logistic regression was adopted to explore factors associated with HB-related RB and behavioral intention (BI). Results: A total of 1299 migrant workers were recruited in the surveys, among whom 29.56% respondents have performed HB-related RB and 85.53% had the BI. 58.19%of respondents having sexual activities never wore a condom. The risk scores of attitudes towards behavior (AB), subjective norms (SN), experience of behavior (EB) and regret feeling (RF) were positively associated with BI, while the sores of AB, EB and BI were positively associated with RB.Conclusions: A considerable proportion of migrant workers have had HB-related risk behaviors or had the behavioral intention. Theory-grounded education, focused on the identified TPB variables, may play a significant role in improving the cognition and behaviors towards HB.
Roadmap for the use of Base Editors to Decipher Drug Mechanism of Action
Authors: Prat Estel Aparicio , Yan Dong , Mariotti Marco , Bassik Michael , Hess Gaelen , Fortin Jean-Philippe , Weston Andrea , Xi Hualin S. , Stanton Robert 
Published: December 2020

Abstract
Background: CRISPR base editors are powerful tools for large-scale mutagenesis studies. This kind of approach can elucidate the mechanism of action of compounds, a key process in drug discovery. Here, we explore the utility of base editors in an early drug discovery context, and we focus on G-protein coupled receptors.Results: We set up a pooled mutagenesis screening framework based on a modified version of the CRISPR-X base editor system. We determine optimized experimental conditions for mutagenesis where sgRNAs are delivered by cell transfection or viral infection over extended time periods (>14 days), resulting in high mutagenesis produced in a short region located at -4/+8 nucleotides with respect to the sgRNA match. We thus target the Beta 2 Adrenergic Receptor (B2AR) and employ a 6xCRE-mCherry reporter system to monitor its activity. The results of our screening indicate that residue 184 of B2AR is crucial for its activation. Based on our experience, we then outline the crucial points to consider when designing and performing CRISPR-based pooled mutagenesis screening, including the typical technical hurdles encountered when studying compound pharmacology. Conclusions: The base editing technology has a great potential to help deciphering the mechanism of action of drugs, and it is a very powerful tool in drug discovery. Here we show an application of pooled mutagenesis screening to study B2AR, and we provide a roadmap for successfully applying this approach to other target proteins.
Diagnostic Model of Covid-19 for Resource-Limited Countries Based on The Combination Of Clinical Symptoms, Chest Radiography and Laboratory Test&nbsp;
Authors: Herikurniawan Herikurniawan , Rumende Martin C , Setiati Siti , Kumalawati July , Deslanthy Belinda Thania 
Published: December 2020

Abstract
Background: COVID-19 is an infection caused by SARS-CoV 2. RT-PCR test is the gold standard for COVID-19 diagnosis, detecting the virus nucleic acid. However, this test is not yet available in every healthcare center, thus making the diagnostic process lengthy. A prompt diagnosis and immediate management are important to decrease mortality and morbidity rate, as well as to prevent transmission. A simple and practical diagnostic model using determinant factors is needed to guide the physician for a quicker decision-making. Method: This study is a cross sectional study using consecutive sampling method. The study was conducted at RSUPN Cipto Mangunkusumo, Jakarta from March to June 2020. History of contact with COVID-19, clinical symptoms, laboratory examination, and chest radiographs data were taken from medical records. The steps of analysis were univariate, bivariate, and multivariate analyses, followed by ROC curve and Hosmer-Lemeshow calibration. Result: There were 187 patients with the majority of subjects in the age group < 60 years old (65,2%) and male sex (53,47%). The most frequent comorbidities were diabetes mellitus and hypertension. The selected variables in this scoring system were contact history, fever/history of fever, dyspnea with respiratory rate >20 breaths/minute, leucocyte ≤ 10.000 cells/mL and typical chest radiography. The area under the curve for this model was 0,777 (CI 95% (0,706-0,847), P<0,001). The probability was 82% with a cut-off point ≥ 4, PPV: 74%, NPV: 77%. Conclusion: Determinant model based on the combination of contact history, fever/history of fever, dyspnea, leucocyte ≤ 10.000 cells/mL and typical chest radiography provides a good accuracy to establish COVID-19 diagnosis.
Nephrology In-Reach Services Lead to Improvement in Mortality in Acute Kidney Injury – 3 (AKI-3)
Authors: Gkekas E. , Tang TYT. , Brazell M. , Brennan M. , Ayub H. , Chernick J. , Jones P. , Robinson A. , Suthermaraj G , Gaunt P. , Ahmed S. , Clark R. , Srivast Shalabh 
Published: December 2020

Abstract
Background: Acute Kidney Injury (AKI) is a sudden decline in kidney function. Early detection and prompt treatment of AKI is vital in improving the outcome of patients. We introduced in-reach nephrology services at South Tyneside District Hospital (STDH) as part of a reconfiguration of local NHS services. Aims: The principal aim of this study is to analyse patient outcomes relating to service developments and to explore prognostic characteristics among a cohort of AKI-3 patients Design: This was a single centre retrospective impact evaluation study.Methods: We studied all patients (n=246) who either presented with or developed AKI-3 during their admission at South Tyneside District Hospital from 2016 to 2018. The inclusion criteria included age 18-95 years and a diagnosis of AKI-3 as per KDIGO classification. Exclusion include those on established dialysis regime or on palliative care. Results: A total of 246 patients were admitted with AKI-3. There were 64 deaths from AKI-3 over the three-year period. Mortality decreased from 29.5% to 20.7% from 2016 to 2018. In patients with Community Acquired (CA-AKI3) the overall mortality rate was 24.2% (n=182), whereas the overall mortality rate of those with Hospital Acquired (HA-AKI3) was 31.3% (n=64). The pre-AKI use of ACEi, A2RB or diuretics increased from 39.7% in 2016 (n=78), to 59.3% in 2017 (n=86) and 64.6% in 2018 (n=82). Conversely, mortality associated with the use of these medications reduced each consecutive year (32.3%, 25.5%, 18.9%).Conclusion: Development of nephrology in-reach services, staff education measures and a primary care pathway could reduce AKI-3 mortality among patients in inpatient and community settings.
Long-term mental health support systems in the aftermath of disasters in Japan – Statistical data of the Miyagi Disaster Mental Health Care Center
Authors: Fukuchi Naru , Chiba Shusaku 
Published: December 2020

Abstract
Background

A long-term mental health support system for the community is sometimes needed following massive natural disasters. Although the Disaster Mental Health Care Center (DMHCC) was established as a long-term mental health care center in Japan, its exact role and functioning are unclear. The Great East Japan Earthquake and Tsunami (GEJE) of 2011 affected thousands of residents. The Miyagi DMHCC was established in Miyagi prefecture in response to the GEJE and supported residents and communities as a long-term mental health care center.
Methods

The main purpose of this study was to clarify which population is psychologically at high risk and which methods are useful for residents’ mental health in each phase. The study used data collected by the Miyagi DMHCC that included personal information of residents who were supported by the center from 2013 to 2018. Chi-square tests of independence were conducted on the annual number of individuals supported by the center, sex, and the number of support methods used by the center according to years. A one-way analysis of variance was conducted on the annual mean age, followed by a post-hoc comparison of the functioning of the center.
Results

The number of residents who needed mental health support dramatically increased in Miyagi prefecture after the 2011 disaster. The Miyagi DMHCC supported 6,850 individuals who sought mental health services, which accounted for 22.9% of all cases reported to the health services between 2012 and 2017. Based on the results, in the first few years, the elderly residents who lived alone were declared as high-risk individuals by the health survey and supported through home visits. Several years later, as younger people started to seek mental health support by themselves, they underwent counseling at the Miyagi DMHCC.
Conclusion

Residents who need mental health support might change depending on recovery phases. Long-term mental health care centers should observe community recovery and provide appropriate support. We discuss the implications of this result and future research directions.
SFRP1 and PTGS2 are potential biomarkers of tumorigenic properties in human placenta-derived mesenchymal stem cells
Authors: Jia Yuanyuan , Ma Xiaona , Yan Xiurui , Xue Jing , Yang Tingting , Liang Xueyun , Liu Xiaoming 
Published: December 2020

Abstract
Background

Mesenchymal stem cells (MSCs) display tumour tropism and have been explored as cellular vehicles to deliver anti-cancer agents. As cellular components of the tumour microenvironment, MSCs also influence tumour progression. However, the tumour transformation-related genes of MSCs are not well understood since either oncogenic or tumour suppressor effects within these cells have been reported. Here, we aimed to identify potential biomarkers capable of tumorigenic risk by RNA-seq analysis of human placenta tissue-derived MSCs (hPTMSCs) exposed to the carcinogenic agent 3-methylcholanthrene (3-MC).
Results

Twenty-nine tumour transformation-related genes and three pluripotency-related genes were identified as differentially expressed genes (DEGs) in hPTMSCs. Importantly, SFRP1 and PTGS2 were further identified as tumour suppressors and oncogenes in hPTMSCs, respectively. The overexpression of SFRP1 and PTGS2 was positively correlated with the expression of the pluripotent markers OCT4, CMYC and NANOG in the human lung adenocarcinoma cell line A549, and the low expression of SFRP1 and PTGS2 was inversely correlated with the expression of the pluripotent markers SOX2, OCT4 and NANOG in hPTMSCs. Interestingly, overexpression of SFRP1 led to reduced cell viability, colony formation and migration of A549 cells. In contrast, the ectopic expression of PTGS2 exerted the opposite effect.
Conclusions

These results indicate that hPTMSCs with high PTGS2 expression but low SFRP1 expression may have more potential tumorigenic capacity. Taken together, this study suggests that PTGS2 and SFRP1 may be valuable biomarkers for quality and safety control of hPTMSC preparations in clinical applications, which warrants further study.
The impact of molded pulp product process on the mechanical properties of molded BCTMP
Authors: Dislaire Claire , Grohens Yves , Seantier Bastien , Muzy Marion 
Published: December 2020

Abstract
This study was carried out using bleached softwood Chemi-Thermo-Mechanical Pulp to evaluate the influence of Molded Pulp Products’ manufacturing process parameters on the finished products’ mechanical and hygroscopic properties. A Taguchi table was done to make 8 tests with specific process parameters such as moulds temperature, pulping time, drying time and pressing time. The results of these tests were used to obtain an optimized manufacturing process with improved mechanical properties and a lower water uptake after sorption analysis and water immersion. The optimized process parameters allowed us to improve the Young’ Modulus after 1h immersion of 58% and a water uptake reduction of 78% with the first 8 tests done.
Cryptosporidiosis modulates gut microbiome metabolism and the immune response in an infected host
Authors: Karpe Avinash V , Hutton Melanie L , Mileto Steven J , James Meagan L , Evans Chris , Shah Rohan M , Ghodke Amol B , Hillyer Katie E , Metcalfe Suzanne S , Liu Jian-Wei , Walsh Tom , Lyras Dena , Palombo Enzo A , Beale David J 
Published: December 2020

Abstract
Background

Cryptosporidiosis is a major global human health concern. Despite well-established methods, misdiagnosis remain common. Resulting incorrect clinical prescription often causes anti-microbial resistance development in the patients. Our understanding of the Cryptosporidium infection mechanism remains limited, compounding the difficulty of clinical diagnosis. Multi-omics approach has shown significant potential for addressing this limitation and, biomarker discovery towards rapid and accurate diagnostics. This study investigated the underlying biochemistry of host-microbiome-parasite relationships during infection.
Methods

C57BL/6J mice were infected with 1 × 105 Cryptosporidium parvum oocysts via oral gavage. Faecal samples were collected daily, while blood, liver tissues and luminal contents of the small and large intestines were collected 10 days post infection. High-resolution liquid chromatography and low-resolution gas chromatography coupled with mass spectrometry were used to analyse the proteomes and metabolomes of faeces, serum, liver, and luminal contents. Faecal samples were additionally subjected to 16S rRNA gene sequencing. Univariate and multivariate statistical analysis were applied to all datasets.
Results

Host and microbial energy pathways altered during infection. Glycolysis/citrate cycle metabolites, such as malate and lactate, were elevated in the large intestine. Short-chain fatty acids, formate and acetate, increased in the small intestine, while butanoate increased in the caecum-colon. This correlated with an increased abundance of bacteria associated with a stressed host environment, including Lactobacillus (small intestine) and Coriobacteriaceae (throughout the intestine, but more prominently in colon). The expression of host electron transfer flavoprotein, phosphoglycerate kinase and acetyl CoA binding proteins, yeast glyceraldehyde-3-phosphate dehydrogenase, and Lactobacillus glyceraldehyde-3-phosphate dehydrogenase significantly increased in the infected gut. Liver oxalate increase was also seen during infection.
Conclusions

The microbiome-parasite relationship is more influential than the previously thought host-parasite relationship, in mediating major biochemical changes in the mouse gut during cryptosporidiosis. Defining this parasite-microbiome interaction is the first step towards building a comprehensive cryptosporidiosis model.
Normal body weight with central obesity increases newly onset cardiovascular disease in adults from rural Northeast China
Authors: Yu Shasha , Guo Xiaofan , Li GuangXiao , Yang Hongmei , Zheng Liqiang , Sun Yingxian 
Published: December 2020

Abstract
Background

Cumulative evidence indicates that normal weight with central obesity (NWCO) is associated with cardiovascular diseases risk factors like hypertension, diabetes, dyslipidemia. However, the possible predictive effect of NWCO on newly onset cardiovascular diseases (CVD) including coronary heart disease (CHD) and stroke has not been studied in detail.
Methods

Participants underwent physical examination at 2012–2013 and followed-up at 2015–2017 who previously diagnosed CVD were excluded. Body mass index (BMI) ≥ 25 kg/m2 defined obesity, waist-to-height ratio ≥ 0.5 defined central obesity. Four groups: Normal weight without (NW) or NWCO, obesity without (OB) or with central obesity (OBCO).
Results

In total, 9439 participants (5006 men and 4433 women). The prevalence of different types of obesity were 39.6% for NW, 16.1% for NWCO, 4.1% for OB and 40.2% for OBCO. NWCO [HR = 1.565, 95%CI = 1.195, 2.051] and OBCO [HR = 1.845, 95%CI = 1.480, 2.300] were significantly associated with increasing risk of newly onset CVD. Besides, NWCO [HR = 2.337, 95%CI = 1.257, 4.344], OB [HR = 3.22, 95%CI = 1.166, 8.905] and OBCO [HR = 2.449, 95%CI = 1.386, 4.326] were all correlated with newly onset CHD but none of them had relationship with newly onset stroke.
Conclusions

We found that NWCO and OBCO were associated with newly onset CVD in rural Northeast Chinese adults. This underscored the effect of central obesity on CVD and suggested that even in individual with normal BMI, it is still necessary to prevent, monitor and control of central obesity.
Problems in measuring the JTC-bias in patients with psychotic disorders with the fish task: A secondary analysis of a baseline assessment of a randomized controlled trial
Authors: Pytlik Nico , Soll Daniel , Hesse Klaus , Moritz Steffen , Bechdolf Andreas , Herrlich Jutta , Kircher Tilo , Klingberg Stefan , Landsberg Martin W. , Müller Bernhard W. , Wiedemann Georg , Wittorf Andreas , Wölwer Wolfgang , Wagner Michael , Mehl Stephanie 
Published: December 2020

Abstract
Background: The jumping to conclusions bias (JTC) is considered to be an important causal factor in theoretical models for the formation and maintenance of delusions. However, recent meta-analytic findings show a rather equivocal pattern of results regarding associations between JTC and delusions. Thus, the present study aims to investigate in a large sample whether the JTC-bias is more pronounced in patients with psychotic disorders in comparison to controls and whether the JTC bias is associated with a more severe delusional conviction, persecutory delusions, and positive symptoms in general.Methods: Patients with psychotic disorders (n = 300) enrolled in a therapy trial and healthy controls (n = 51) conducted a variant of the beads task (fish task) as a measure for the JTC-bias at the start of the trial. Further, clinical interviews were used to assess patients’ delusional severity and delusional conviction. Results: There were no statistically significant differences between patients with psychotic disorders (with 53% displaying the JTC-bias) and controls (41%). Furthermore, there were no statistically significant correlations between JTC measures and persecutory delusions, delusional conviction, and positive symptoms.Conclusions: We found no differences in JTC between patients with psychotic disorders and healthy controls, which is in part in line with meta-analytic findings using a wide range of JTC task variants. Interestingly, patients with psychotic disorders displayed JTC rates commonly found in the literature, while healthy control subjects showed an unexpectedly high level of JTC. The task variant we used in the present study (fish task) is discussed as a potential reason for our results, as it may induce a more deliberative reasoning style in controls as compared to the traditional beads task. Furthermore, possible implications for the measurement of the JTC-bias, in general, are discussed.Trial Registration: ISRCTN29242879 (isrctn.com), date of registration: April 12th 2006, retrospectively registered