bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cognitive load amplifies Parkinson’s tremor through excitatory network influences onto the thalamus
Authors: Dirkx Michiel F , Zach Heidemarie , van Nuland Annelies J , Bloem Bastiaan R , Toni Ivan , Helmich Rick C 
Published: March 2020

Abstract
Parkinson’s tremor is related to cerebral activity in both the basal ganglia and a cerebello-thalamo-cortical circuit. It is a common clinical observation that tremor markedly increases during cognitive load (such as mental arithmetic), leading to serious disability. Previous research has shown that this tremor amplification is associated with reduced efficacy of dopaminergic treatment. Understanding the mechanisms of tremor amplification and its relation to catecholamines might help to better control this symptom with a targeted therapy. We reasoned that, during cognitive load, tremor amplification might result from modulatory influences onto the cerebello-thalamo-cortical circuit controlling tremor amplitude, from the ascending arousal system (bottom-up), a cognitive control network (top-down), or their combination. We have tested these hypotheses by measuring concurrent EMG and functional MRI in 33 patients with tremulous Parkinson’s disease, OFF medication, during alternating periods of rest and cognitive load (mental arithmetic). Simultaneous heart rate and pupil diameter recordings indexed activity of the arousal system (which includes noradrenergic afferences). As expected, tremor amplitude correlated with activity in a cerebello-thalamo-cortical circuit; and cognitive load increased tremor amplitude, pupil diameter, heart rate, and cerebral activity in a cognitive control network distributed over fronto-parietal cortex, insula, thalamus and anterior cingulate cortex. The novel finding, obtained through network analyses, indicates that cognitive load influences tremor by increasing activity in the cerebello-thalamo-cortical circuit in two different ways: by stimulating thalamic activity, likely through the ascending arousal system (given that this modulation correlated with changes in pupil diameter), and by strengthening connectivity between the cognitive control network and the cerebello-thalamo-cortical circuit. We conclude that both the bottom-up arousal system and a top-down cognitive control network amplify tremor when a Parkinson’s patient experiences cognitive load. Interventions aimed at attenuating noradrenergic activity or cognitive demands may help to reduce Parkinson’s tremor.
Thinking fast or slow? Functional magnetic resonance imaging reveals stronger connectivity when experienced neurologists diagnose ambiguous cases
Authors: van den Berg Berry , de Bruin Anique B H , Marsman Jan-Bernard C , Lorist Monicque M , Schmidt Henk G , Aleman André , Snoek Jos W 
Published: March 2020

Abstract
For ∼40 years, thinking about reasoning has been dominated by dual-process theories. This model, consisting of two distinct types of human reasoning, one fast and effortless and the other slow and deliberate, has also been applied to medical diagnosis. Medical experts are trained to diagnose patients based on their symptoms. When symptoms are prototypical for a certain diagnosis, practitioners may rely on fast, recognition-based reasoning. However, if they are confronted with ambiguous clinical information slower, analytical reasoning is required. To examine the neural underpinnings of these two hypothesized forms of reasoning, 16 highly experienced clinical neurologists were asked to diagnose two types of medical cases, straightforward and ambiguous cases, while functional magnetic resonance imaging was being recorded. Compared with reading control sentences, diagnosing cases resulted in increased activation in brain areas typically found to be active during reasoning such as the caudate nucleus and frontal and parietal cortical regions. In addition, we found vast increased activity in the cerebellum. Regarding the activation differences between the two types of reasoning, no pronounced differences were observed in terms of regional activation. Notable differences were observed, though, in functional connectivity: cases containing ambiguous information showed stronger connectivity between specific regions in the frontal, parietal and temporal cortex in addition to the cerebellum. Based on these results, we propose that the higher demands in terms of controlled cognitive processing during analytical medical reasoning may be subserved by stronger communication between key regions for detecting and resolving uncertainty.
Measurement of Serum Neuron-Specific Enolase in Neuroblastoma: Is There a Clinical Role?
Authors: Ferraro Simona , Braga Federica , Luksch Roberto , Terenziani Monica , Caruso Simone , Panteghini Mauro 
Published: March 2020

AbstractBACKGROUNDThe measurement of neuron-specific enolase (NSE) in serum is frequently requested for diagnosis, risk stratification, and treatment monitoring of neuroblastoma (NB) in the pediatric population. However, authoritative clinical practice guidelines advise about the poor diagnostic performance of NSE.ContentWe critically appraised the available literature evaluating the diagnostic and prognostic value of NSE in the management of NB, paying special attention to the definition of appropriate threshold levels. In addition, we discuss the interfering conditions causing artifactual increases of NSE concentrations in serum and potentially influencing the clinical evaluation of patients with suspected NB.SummaryNo definitive evidence supports the use of serum NSE for diagnosis and monitoring of NB. The risk of obtaining false-positive NSE results associated with confounders (e.g., sample hemolysis) and other pathophysiologic conditions (e.g., inflammation) is remarkable and hampers the diagnostic value of this test. NSE may be helpful to define the risk of death of patients with NB, mainly in the advanced stages of disease. However, further studies validating currently marketed immunoassays and defining threshold values useful for this scope are warranted.
Variations in incidence of venous thromboembolism in low-, middle-, and high-income countries
Authors: Siegal Deborah M , Eikelboom John W , Lee Shun Fu , Rangarajan Sumathy , Bosch Jackie , Zhu Jun , Yusuf Salim ,   the Venous Thromboembolism Collaboration
Published: March 2020

Abstract

Aims
To examine the rates of venous thromboembolism (VTE) in high-income, upper middle-income, and lower middle/low-income countries (World Bank Classification).

Methods and results
We examined the rates of VTE in high-income, upper middle-income, and lower middle/low-income countries (World Bank Classification) in a cohort derived from four prospective international studies (PURE, HOPE-3, ORIGIN, and COMPASS). The primary outcome was a composite of pulmonary embolism, deep vein thrombosis, and thrombophlebitis. We calculated age- and sex-standardized incidence rates (per 1000 person-years) and used a Cox frailty model adjusted for covariates to examine associations between the incidence of VTE and country income level. A total of 215 307 individuals (1.5 million person-years of follow-up) from high-income (n = 60 403), upper middle-income (n = 42 066), and lower middle/low-income (n = 112 838) countries were included. The age- and sex-standardized incidence rates of VTE per 1000 person-years in high-, upper middle-, and lower middle/low-income countries were 0.87, 0.25, and 0.06, respectively. After adjusting for age, body mass index (BMI), smoking, antiplatelet therapy, anticoagulant therapy, education level, ethnicity, and incident cancer diagnosis or hospitalization, individuals from high-income and upper middle-income countries had a significantly higher risk of VTE than those from lower middle/low-income countries [hazard ratio (HR) 3.57, 95% confidence interval (CI) 2.40–5.30 and HR 2.27, 95% CI 1.59–3.23, respectively]. The effect of country income level on VTE risk was markedly stronger in people with a lower BMI, hypertension, diabetes, non-White ethnicity, and higher education.

Conclusion
The rates of VTE are substantially higher in high-income than in low-income countries. The factors underlying the increased VTE risk in higher-income countries remain unknown.
The remodelling index risk stratifies patients with hypertensive left ventricular hypertrophy
Authors: Le Thu-Thao , Lim Vanessa , Ibrahim Rositaa , Teo Muh-Tyng , Bryant Jennifer , Ang Briana , Su Boyang , Aw Tar-Choon , Lee Chi-Hang , Bax Jeroen , Cook Stuart , Chin Calvin W L 
Published: March 2020

Abstract

Aims
Hypertensive left ventricular hypertrophy (LVH) is associated with increased cardiovascular events. We previously developed the remodelling index (RI) that incorporated left ventricular (LV) volume and wall-thickness in a single measure of advanced hypertrophy in hypertensive patients. This study examined the prognostic potential of the RI in reference to contemporary LVH classifications.

Methods and results
Cardiovascular magnetic resonance was performed in 400 asymptomatic hypertensive patients. The newly derived RI (EDV3t, where EDV is LV end-diastolic volume and t is the maximal wall thickness across 16 myocardial segments) stratified hypertensive patients: no LVH, LVH with normal RI (LVHNormal-RI), and LVH with low RI (LVHLow-RI). The primary outcome was a composite of all-cause mortality, acute coronary syndromes, strokes, and decompensated heart failure. LVHLow-RI was associated with increased LV mass index, fibrosis burden, impaired myocardial function and elevated biochemical markers of myocardial injury (high-sensitive cardiac troponin I), and wall stress. Over 18.3 ± 7.0 months (601.3 patient-years), 14 adverse events occurred (2.2 events/100 patient-years). Patients with LVHLow-RI had more than a five-fold increase in adverse events compared to those with LVHNormal-RI (11.6 events/100 patient-years vs. 2.0 events/100 patient-years, respectively; log-rank P &lt; 0.001). The RI provided incremental prognostic value over and above a model consisting of clinical variables, LVH and concentricity; and predicted adverse events independent of clinical variables, LVH, and other prognostic markers. Concentric and eccentric LVH were associated with adverse prognosis (log-rank P = 0.62) that was similar to the natural history of hypertensive LVH (5.1 events/100 patient-years).

Conclusion
The RI provides prognostic value that improves risk stratification of hypertensive LVH.
Long-term adaptive versus maladaptive remodelling of the pulmonary autograft after the Ross operation
Authors: Yacoub Magdi H , Tsang Victor , Sarathchandra Padmini , Jensen Hanna , Hughes Sian , Latif Najma 
Published: March 2020

Abstract

OBJECTIVES
Following the Ross operation, the pulmonary autograft undergoes structural changes (remodelling). We sought to determine the extent, nature and possible determinants of long-term remodelling in the different components of the pulmonary autograft.

METHODS
Ten pulmonary autografts and 12 normal control valves (6 pulmonary and 6 aortic) were examined by conventional histology, immunocytochemistry and electron microscopy. The structural changes were quantified by morphometry.

RESULTS
The leaflets from free-standing root replacement valves demonstrated thickening to levels comparable to the normal aortic leaflets, largely due to the addition of a thin layer of ‘neointima’ formed of radial elastic fibres, collagen bundles and glycoaminoglycans, on the ventricular aspect of the leaflets. The leaflets of valves from sub-coronary implantation demonstrated a significantly thicker fibroelastic layer on the ventricularis and calcium deposition in the fibrosa. The media of the explanted valves showed increased number of lamellar units to levels comparable to normal aortic roots. Electron microscopy of valves inserted as free-standing roots showed increased organization into continuous layers. However, intralamellar components showed varying degrees of ‘disorganization’ in comparison to those in the normal aortic media. In addition, there was a marked increase in the number of vasa vasorum with thickened arteriolar wall in the outer media and adventitia.

CONCLUSIONS
Following the Ross operation, in the very long term, all components of the autograft showed varying degrees of remodelling, which was judged to be largely adaptive. Defining the type, determinants and possible functional effects of remodelling could help in understanding and optimizing the results of the Ross operation.
Investigation of the roles of AgrA and σB regulators in Listeria monocytogenes adaptation to roots and soil
Authors: Marinho Catarina M , Garmyn Dominique , Gal Laurent , Brunhede Maja Z , O'Byrne Conor , Piveteau Pascal 
Published: March 2020

ABSTRACT
Little is known about the regulatory mechanisms that ensure the survival of the food-borne bacterial pathogen Listeria monocytogenes in the telluric environment and on roots. Earlier studies have suggested a regulatory overlap between the Agr cell–cell communication system and the general stress response regulator σB. Here, we investigated the contribution of these two systems to root colonisation and survival in sterilised and biotic soil. The ability to colonise the roots of the grass Festuca arundinacea was significantly compromised in the double mutant (∆agrA∆sigB). In sterile soil at 25°C, a significant defect was observed in the double mutant, suggesting some synergy between these systems. However, growth was observed and similar population dynamics were shown in the parental strain, ΔagrA and ΔsigB mutants. In biotic soil at 25°C, viability of the parental strain declined steadily over a two-week period highlighting the challenging nature of live soil environments. Inactivation of the two systems further decreased survival. The synergistic effect of Agr and σB was stronger in biotic soil. Transcriptional analysis confirmed the expected effects of the mutations on known Agr- and σB-dependent genes. Data highlight the important role that these global regulatory systems play in the natural ecology of this pathogen.
Challenging the charge balance hypothesis: reconsidering buffer effect and reuptake of previously excreted organic acids by Penicillium ochrochloron
Authors: Artmann D J , Vrabl P , Gianordoli R , Burgstaller W 
Published: March 2020

ABSTRACT
Penicillium ochrochloron was used in the past for the leaching of zinc from a zinc oxide containing filter dust via excreted organic acids. Organic acid excretion by P. ochrochloron was stimulated by the addition of an extracellular buffer (2-(N-Morpholino)ethanesulfonic acid, MES; or zinc oxide, ZnO: ZnO + 2 H+ → Zn2+ + H2O). It was tested if the buffer stimulated excretion of organic acid anions is due to the necessity of an anion efflux across the plasma membrane to maintain electroneutrality by balancing the excretion of protons by the H+-ATPase. This charge balance hypothesis was previously postulated for P. ochrochloron. Two strains of P. ochrochloron were studied, which differed in growth parameters and amount of excreted organic acids. From the results, it was concluded that charge balance at the plasma membrane is not the main reason for organic acid excretion in these two strains of P. ochrochloron. Furthermore, the phenomenon of reuptake of excreted organic acids in the presence of about 100 mM of glucose is confirmed. It is suggested that the equilibrium between extracellular and intracellular organic acid anions may be maintained passively by a facilitated diffusion transporter.
Conversion of waste materials into very long chain fatty acids by the recombinant yeast Yarrowia lipolytica
Authors: Gajdoš Peter , Hambalko Jaroslav , Slaný Ondrej , Čertík Milan 
Published: March 2020

ABSTRACT
Erucic acid (C22:1Δ13) has several industrial applications including its use as a lubricant, surfactant and biodiesel and composite material constituent. It is produced by plants belonging to the Brassicaceae family, especially by the high erucic acid rapeseed. The ability to convert oleic acid into erucic acid is facilitated by FAE1. In this study, FAD2 (encoding Δ12-desaturase) was deleted in the strain Po1d to increase oleic acid content. Subsequently, FAE1 from Thlaspi arvense was overexpressed in Yarrowia lipolytica with the Δfad2 genotype. This resulted in the YL10 strain producing very long chain fatty acids, especially erucic acid. The YL10 strain was cultivated in media containing crude glycerol and waste cooking oil as carbon substrates. The cells grown using glycerol produced microbial oil devoid of linoleic acid, which was enriched with very long chain fatty acids, mainly erucic acid (9% of the total fatty acids). When cells were grown using waste cooking oil, the highest yield of erucic acid was obtained (887 mg L–1). However, external linoleic and α-linolenic were accumulated in cellular lipids when yeasts were grown in an oil medium. This study describes the possibility of conversion of waste material into erucic acid by a recombinant yeast strain.
Reducing error in small-area estimates of multi-source forest inventory by multi-temporal data fusion
Authors: Katila Matti , Heikkinen Juha 
Published: March 2020

Abstract
Since the 1990s, forest resource maps and forest variable estimates for small areas have been produced by combining national forest inventory (NFI) field plot data, optical satellite images and numerical map data. A non-parametric $k$-NN method has frequently been employed. In Finland, such multi-source NFI (MS-NFI) forest variable estimates for municipalities have been produced eight times. A relatively large variation has been observed between subsequent estimates. In this study, a large-scale evaluation of small-area estimates from an MS-NFI conducted in 2013 was carried out in comparison with pure NFI field data-based estimates and error estimates. The proportion of municipalities with significant differences was larger than expected, e.g. over 10% for the mean volume, which indicates systematic error in the small-area estimates. A multi-temporal data fusion combining MS-NFI estimators from three time points—2011, 2013 and 2015—was tested as a means to improve single time point MS-NFI estimates of the mean volumes of growing stock and of tree species groups. A generalized least squares (GLS) technique and unweighted averaging were tested. The improvement was small but consistent when validated against the NFI field data-based estimates for the municipalities. The unweighted averaging worked nearly as well as a GLS estimator.
Variation in leaf area index in complex mixed-conifer forests in California’s Sierra Nevada: implications for stocking control
Authors: O’Hara Kevin L , Battles John J 
Published: March 2020

AbstractThe mixed-conifer forests in California’s Sierra Nevada include species from several genera (Pinus, Abies, Pseudotsuga, Calocedrus and Sequoiadendron). These forests have complex disturbance regimes dominated by low to moderate severity fire that often resulted in patchy spatial patterns and multiaged stands. Leaf area index (LAI) describes the total leaf surface area per unit area in a forest community and is related to wood and biomass production and ecosystem values such as water usage, water yields and carbon sequestration. LAI can also serve as a representation of growing space occupancy and the basis for stocking control, including in multiaged stands. Nine study sites were sampled with 22–37 0.05 ha plots per study site to estimate LAI and other metrics. LAI was highest in study sites with greater proportions of shade tolerant Abies and Calocedrus species and on higher productivity sites. Recent drought-related mortality has reduced stocking and LAI. The combination of fire suppression and timber harvest over the past century has resulted in stands with higher densities, and greater proportions of shade tolerant species. Managing these structures to restore their presettlement character will involve reducing overall stocking, increasing proportions of intolerant species and increasing fine-scale heterogeneity. LAI allocation—allocating leaf area to age classes, species or canopy strata—can be used to design new structures that resemble presettlement structures and are resilient to disturbances.
South Asian Patients With Inflammatory Bowel Disease in the United States Demonstrate More Fistulizing and Perianal Crohn Phenotype
Authors: Jangi Sushrut , Ruan Alex , Korzenik Joshua , de Silva Punyanganie 
Published: March 2020

AbstractBackgroundSouth Asians have recently been identified as having a rapidly rising incidence and prevalence of inflammatory bowel disease (IBD) throughout the world. However, longitudinal phenotypic studies of South Asians living in the United States remain scarce.MethodsWe retrospectively studied 171 South Asian patients with IBD treated at 2 US tertiary centers who presented between 2000 and 2016. South Asian IBD patients were randomly matched in a 1:2 ratio with sex and IBD subtype–matched (ulcerative colitis [UC] vs Crohn disease [CD]) white control patients (n = 342). Demographic and phenotypic characteristics were evaluated and compared between the 2 groups. Odds ratios (OR), logistic regression, and survival analysis were performed using R studio and STATA.Results81 South Asian patients and 162 white patients had CD, and 90 South Asians and 180 white patients had UC. Among the CD group, South Asian patients were diagnosed at a median older age (age 28) than white patients (21 years; P &lt; 0.003). Fistulizing disease (24.1% vs 8.6%; P &lt; 0.002), perianal disease (20.3% vs 2.5%; P &lt; 0.005), and presentation of rectal pain (16.2% vs 2.9%; P &lt; 0.001) were more common among South Asian patients with CD than among white patients. After adjusting for covariates, South Asian patients with CD were less likely to be placed on thiopurines (OR = 0.36; P &lt; 0.007) or to receive more than 1 biologic (OR = 0.42; P &lt; 0.040). South Asian patients with UC were less likely to have proctitis (10% vs 22.2%; P &lt; 0.022) and more likely to have primary sclerosing cholangitis (n = 7 vs n = 2; P &lt; 0.007). South Asian patients born in the United States or those who had migrated before age 5 were younger at the age of IBD diagnosis (age 18.9 vs 32.4; P &lt; 0.0005).ConclusionWe found unique demographic and phenotypic characteristics among South Asian patients, including more penetrating disease in those with CD and less proctitis among those with UC, along with altered medication use patterns. Distinct environmental exposures and a potentially unique genetic profile of South Asian patients may confer this variable phenotypic expression, influencing management of this increasingly at-risk population.
Oracle inequalities for square root analysis estimators with application to total variation penalties
Authors: Ortelli Francesco , van de Geer Sara 
Published: March 2020

AbstractThrough the direct study of the analysis estimator we derive oracle inequalities with fast and slow rates by adapting the arguments involving projections by Dalalyan et al. (2017, Bernoulli, 23, 552–581). We then extend the theory to the square root analysis estimator. Finally, we focus on (square root) total variation regularized estimators on graphs and obtain constant-friendly rates, which, up to log terms, match previous results obtained by entropy calculations. We also obtain an oracle inequality for the (square root) total variation regularized estimator over the cycle graph.
An Action of the Polishchuk Differential Operator via Punctured Surfaces
Authors: Drummond-Cole Gabriel C , Tavakol Mehdi 
Published: March 2020

Abstract
For a family of Jacobians of smooth pointed curves, there is a notion of tautological algebra. There is an action of ${\mathfrak{s}}l_2$ on this algebra. We define and study a lifting of the Polishchuk operator, corresponding to ${\mathfrak{f}} \in{\mathfrak{s}}l_2$, on an algebra consisting of punctured Riemann surfaces. As an application, we compare a class of tautological relations on moduli of curves, discovered by Faber and Zagier and relations on the universal Jacobian. We prove that the so called top Faber–Zagier relations come from a class of relations on the Jacobian side.