bluuebunny/crossref_metadata_2025_split huggingface dataset:
WITHDRAWN: Sleep Treatment Improves Neuropsychological Symptoms and Reduces Blood Aβ42/pTau Protein in Alzheimer's Disease Patients: a Longitudinal Study
Authors: Li Benyi , Huang Haihua , Li Mingqiu , Zhang Menglin , Qiu Jiang , Chen Haiyan , Mou Xin , Chen Qinghong , Li Ting C. , Peng Jun 
Published: January 2021

Abstract
Background: Amyloid β peptide-42 (Aβ42) and phosphorylated Tau on Threonine 181 (Tau-pT181) are the core biomarkers in Alzheimer’s disease. Accumulated evidence showed an aberrant elevation of these biomarkers due to sleep disturbance. However, it is not clear if improving sleep quality reduces Aβ42 and Tau-pT181 in Alzheimer’s disease patients.Methods: A longitudinal study was conducted on 126 patients with mild-moderate dementia due to Alzheimer’s disease. Sleep quality was assessed using the Pittsburgh Sleep Quality Index (PSQI). Behavioral and neuropsychological assessment was conducted using multiple self-reporting questionnaire score systems. Aβ42 and Tau-pT181 levels in blood specimens were measured using an ELISA assay kit. All patients received Donepezil treatment for Alzheimer’s disease. Sleep disorders were individually managed with either medication or physical therapy according to their symptom categories.Results: Of the 126 cases, 93 (73.8%) patients were diagnosed with sleep disorders. The PSQI scores significantly correlated with depression and anxiety scores, as well as Aβ42 and Tau-pT181 levels. Also, a significant correlation was found among depression, anxiety, Aβ42 and Tau-pT181 in all patients. Sleep intervention drastically reduced PSQI score, as well as Aβ42 and Tau-pT181 levels, in parallel with improved cognition, deterioration and anxiety scores. Dementia and depression scores were improved only in patients who completely recovered (PSQI < 7) after sleep treatment. There was a 35.9% reduction of Aβ42 levels and a 32.8% reduction of Tau-pT181 levels in this subgroup of patients (56 cases). Conversely, the reduction extent was only 6.9% for Aβ42 and 12.2% for Tau-pT181 in patients who did not completely recover (PSQI ≥ 7 post treatment, 37 cases). Multiple logistic regression analysis revealed that Aβ42 level is the strongest risk factor for sleep disorder incidence and incomplete recovery after sleep treatment.Conclusion: Sleep quality score is associated with patient depression and anxiety status, as well as blood Aβ42 and Tau-pT181 levels. A complete recovery is critical for a full improvement of all behavioral and neuropsychological assessments, which is also associated with a deep reduction of Aβ42 and Tau-pT181 levels. Aβ42 level is a prognostic factor for a diagnosis of sleep disorder and treatment responsiveness.
Bibliometric Analysis of Publication Hot Topics of Smartphones in the Field of Health and Medical Services
Authors: Tian Zhiqiang , Wang Yanjun , Lu Jiao , Wang Chunfang , Zheng Jianzhong 
Published: January 2021

Abstract
Background: Smartphones have captured the attention of many researchers in the field of health and medical services. This study aimed to quantify research ‘hotspots’ in this field, analyse the relationship between research hotspots and the resulting knowledge groups, and provide visual representations of the findings. Methods: Using bibliometric analysis software tools for keyword frequency analysis, we identified research hotspots using keywords from PubMed entries from a 14-year period. The analyses of hotspots were performed using keyword co-occurrence analysis, social network analysis, principal component analysis (PCA), multidimensional scaling analysis, and network visualization technology. Results: The results confirmed that the number of articles has been increasing each year. The topics of mobile applications, telemedicine, self-care, Diabetes Mellitus, treatment outcomes, health promotion, and patient satisfaction associated with smartphones were highlighted. The 35 high-frequency keywords that were extracted constituted five principal components of research related to information technology and telemedicine, diabetes, t-health promotion, and smartphones/handheld computers. Figures of knowledge network maps and perceptual maps show the relationship between the high-frequency keywords. Conclusions: The research of smartphones for health and medical services has experienced significant growth and expansion in knowledge in the last 14 years. Research hotspots for smartphone-related information technology, telemedicine, and health promotion have broad prospects for development. Moreover, this study provides directions for research hotspots and future research in the field of smartphone applications for health and medical services.
Molecular Characterization of Advanced Colorectal Cancer Using Serum Proteomics and Metabolomics
Authors: Rao Jun , Wan Xianghui , Tou Fangfang , He Qinsi , Xiong Aihua , Chen Xinyi , Hou Li , Zheng Zhi 
Published: January 2021

Abstract
Introduction Colorectal cancer (CRC) is a growing public health concern with high mortality rate. However, there are no valid diagnostic biomarkers and few therapeutic strategies available for CRC, especially advanced CRC, since the pathogenic mechanisms remain poorly understood. Objective To comprehensively reveal molecular characterization of advanced CRC, we applied integrated proteomic and metabolomic analyses on serum samples from 20 patients with CRC at stage III or IV. Methods In the present study, we took advantage of nanoscale liquid chromatography and quadrupole time-of-flight tandem mass spectrometry (nanoLC/Q-TOF-MS/MS) and ultraperformance LC/Q-TOF-MS/MS technologies. Results Overall, 551 proteins and 719 metabolites were identified in those serum samples, respectively. Hierarchical clustering analysis indicated much more remarkable diversity in proteomic profiles than metabolomic profiles. Further functional analysis suggested that ten key pathways associated with cancer cell metabolism were dissected including glycolysis/gluconeogenesis, biosynthesis of amino acids, glutathione metabolism, and arachidonic acid metabolism, based on which protein-protein interaction network analysis was thus constructed with 80 proteins and 21 metabolites. Moreover, the regulatory network in advanced CRC was established according to correlation analysis, indicating conserved roles of metabolome and lipids & lipid like molecules in human serum. Nevertheless, three metabolites and two proteins including hydroquinone, leucenol and sphingomyelin were supposed to be potential biomarkers, which were determined to be positively and significantly correlated with CEA and/or CA 19-9.Conclusions Altogether, our work not only extended our understanding on the physiopathology of advanced CRC, but provided potential biomarkers to improve the accuracy of the diagnosis and monitoring of the syndrome.
WITHDRAWN: A Cohort Study of Inferior Vena Cava Thrombosis in Taiwan: An Under-recognized Entity
Authors: Lin Hsuan-Yu , Lin Ching-Yeh , Shen Ming-Ching 
Published: January 2021

Abstract
Background: Inferior vena cava thrombosis (IVCT) is a rare clinical condition. Herein, we report eight cases of IVCT in Taiwanese patients.Methods: Eight Taiwanese patients diagnosed with IVCT between May 2012 and December 2019 were included in this study. The patients’ demographics, presenting characteristics, additional sites of venous thromboembolism, extent of IVCT, prothrombotic risk factors, and IVCT-related adverse events were evaluated.Results: All eight patients with IVCT presented with other coexisting venous thromboembolic manifestations, such as deep venous thrombosis (DVT, 100%) or pulmonary embolism (62.5%). The clinical presentations, including DVT in both lower extremities coexisting with the dilatation of the superficial veins of the abdominal wall (50%), were reported. No congenital anomalies of the inferior vena cava (IVC) were noted. Various thromboembolic risk factors, such as unretrieved IVC filters (25%), pregnancy (37.5%), lupus anticoagulants (37.5%), surgery (25%), antithrombin deficiency (12.5%), hemoglobin H disease (12.5%), and essential thrombocythemia (12.5%), were identified. All patients were administered anticoagulants. One patient (12.5%) developed post-thrombotic syndrome. No mortality was reported in our cohort. Conclusions: This is the first report of IVCT in Taiwanese patients. Typical clinical features of IVC occlusion coexisting with predisposing factors of venous thrombosis, such as lupus anticoagulants, pregnancy, or unretrieved IVC filters, could indicate a diagnosis of IVCT. Moreover, IVCT presenting as a complication resulting from the unretrieved IVC filter was observed, highlighting the potential risks of chronic indwelling filters.
Comparison of Recurrence of Non-muscle Invasive Bladder Carcinoma after Transurethral Resection with Hexaminolevulinate Photodynamic Diagnosis or Regular Cystoscopy
Authors: Hoogeveen Fokke , Blanker Marco , Cauberg Evelyne , Steffens Martijn 
Published: January 2021

Abstract
Purpose: Comparison of recurrence of non-muscle invasive bladder carcinoma after transurethral resection with hexaminolevulinate photodynamic diagnosis (PDD) or regular white light cystoscopy (WLC).Methods: We included patients with newly suspected non-muscle invasive bladder carcinoma in this retrospective cohort study and compared those undergoing transurethral resection by WLC and PDD. The primary outcome was the difference in the recurrence rate after 60 months’ follow-up, but we also stratified recurrence by risk groups. The mean recurrence-free survival was compared between the cohorts. Odds ratios or hazard ratios are reported with 95% confidence intervals.Results: The WLC and PDD cohorts comprised 124 and 91 subjects, respectively. There were no significant differences in recurrence rates at 6 months (recurrence rate 9/123; 7.3%), 12 months (17/118; 14.4%), or 60 months (39/102; 38.2%), with odds ratios of 1.23 (0.48–3.25; P=0.64), 1.32 (0.67–2.62; P=0.42), and 1.12 (0.70–1.79; P=0.65), respectively. Further analysis showed no significant effect of PDD on recurrence by risk group or on mean recurrence-free survival (hazard ratio, 1.12; 0.70–1.79).Conclusion: Photodynamic diagnosis with hexaminolevulinate did not reduce the recurrence of non-muscle invasive bladder carcinoma compared to standard white light cystoscopy when used for transurethral resection.
Evolution Characteristics and Simulation Prediction of Forest and Grass Landscape Fragmentation Based on the “Grain for Green” Projects on the Loess Plateau, P.R. China
Authors: Gu Li , Gong Zhiwen , Bu Yuankun 
Published: January 2021

Abstract
Forest fragmentation is one of the major environmental issues that the international community is generally concerned about under the background of global climate change. Studying the impact and the interaction mechanism of land use change processes on landscape fragmentation is important to gaining a comprehensive understanding of the ecosystem response to human activities and global climate change. Based on the implementation background for the “Grain for Green” Project, we selected the Loess Plateau as the research area and used the coupled future land use simulation (FLUS) model and landscape fragmentation model to explore the temporal and spatial changes in forest and grass landscape fragmentation. The results showed that (1) Woodland, grassland, and cropland are the main landscape types, accounting for about 90% of the total area. In addition, the area of cropland initially increased and then decreased, while the area of woodland and grassland exhibited the opposite trend Oover the last 35 years. In particular, the period from 2000 to 2015 was a forest and grass restoration stage, and the average annual rate of forest and grass restoration reached 0.56%. (2) The FLUS model was used to predict the land use on the Loess Plateau in 2030. The kappa coefficient was 0.85, and the figure of merit coefficient (FOM) was 0.11 for a 1% random sampling, which are within a reasonable range, and the simulation results are also consistent with the objective change in the current social and economic development. (3) The fragmentation of woodland and grassland were dominated by edge type and core type. The core type had a concentrated distribution and an absolute advantage, accounting for more than 75% of the total area. It is predicted that the landscape fragmentation will gradually slowdown in 2030 under different intensities of the “Grain for Green” project. The dynamics of landscape fragmentation based on land use changes are conducive to the reasonable planning and objective evaluation of woodland and grassland spatial allocation and quality improvement, and provide an important basis for the formulation of ecological protection and land management policies.
Candidate Genes Identified by Constructing a Weighted Gene Co-Expression Network and Key Prognostic Factors of Pancreatic Adenocarcinoma
Authors: Xu Peng , Wang Shanjuan , Wang Yongchen 
Published: January 2021

Abstract
BackgroundPancreatic adenocarcinoma(PAAD) is a kind of terribly malignant tumor of the digestive tract with high mortality globally, however, the pathogenic molecular mechanisms of which remain unclear currently. Therefore, it is very important to explore the underlying mechanisms and screen for novel prognostic markers and treatment targets. We performed a weighted gene co-expression network analysis(WGCNA) to identify hub genes associated with clinical parameters of interest using The Cancer Genome Atlas(TCGA) data. In addition, Gene ontology(GO) enrichments and survival analyses were conducted.MethodsThe 14 datasets including gene expression profiles and clinical phenotypes for PAAD patients in TCGA were analyzed by WGCNA in R. After constructing the scale-free network of gene co-expression, modules with clinical significance were distinguished, and hub genes were validated, which also key genes regulated relevant traits. GO enrichments were performed on the modules. Furthermore, the significance of hub genes was confirmed via survival analysis.ResultsWe discovered 12 candidate genes in total which had significant effects on the overall survival of PAAD patients. The expression of DUSP26, TCEAL2, CELF3, CDK5R2, NAP1L2, INA had strong and positive influences on the pathological T stage, while the expression of CEP55, HJURP, KIF23, CDCA5, SAC3D1, ATP6L had great negative impacts on the histological stage. Meanwhile, we also found that the tumor primary location and age of onset were key factors affecting the prognosis of PAAD.ConclusionThese candidate genes probably play important roles in proliferation, differentiation and metastasis of PAAD cells, which may be acted as novel prognostic markers and effective therapeutic targets. Besides, most of them are first reported in PAAD and deserved in-depth research.
Granulocyte Colony-Stimulating Factor-Induced TAF9 Modulates P53-TRIAP1-CASP3 Axis to Prevent Retinal Ganglion Cell Death after Optic Nerve Ischemia
Authors: Wen Yao-Tseng , Lin Keh-Liang , Huang Chin-Te , Tsai Rong Kung 
Published: January 2021

Abstract
BackgroundOptic nerve head (ONH) infarct can result in progressive retinal ganglion cell (RGC) death. Some evidences indicated that the granulocyte colony-stimulating factor (GCSF) provides positive effects against ischemic damage on RGCs. However, protective mechanisms of the GCSF after ONH infarct are complex and remain unclear. MethodsTo investigate the complex mechanisms, the transcriptome profiles of the GCSF-treated retinas were examined using microarray technology. The retinal mRNA samples on days 3 and 7 post rat anterior ischemic optic neuropathy model (rAION) were analyzed by microarray and bioinformatics analyses. To evaluate the TAF9 function in RGC apoptosis, GCSF plus TAF9 siRNA-treated rats were evaluated using retrograde labeling with FluoroGold assay, TUNEL assay, and Western blotting in a rAION.ResultsGCSF treatment influenced 3101 genes and 3332 genes on days 3 and 7 post rAION, respectively. ONH infarct led to changes in 702 and 179 genes on days 3 and 7 post rAION, respectively. After cluster analysis, the TATA box-binding protein (TBP)-associated factor levels were significantly reduced after ONH infarct but significantly increased after GCSF treatment. The network analysis revealed that TBP associated factor 9 (TAF9) can bind to TP53 to induce TP53 regulated inhibitor of apoptosis 1 (TRIAP1) expression. The RGC density in the GCSF plus TAF9 siRNA-treated group was 1.95-fold (central retina) and 1.75-fold (midperipheral retina) lower than that in the GCSF-treated group (p < 0.05). The number of apoptotic RGC in the GCSF plus TAF9 siRNA-treated group is threefold higher than that in the GCSF-treated group (p < 0.05). Treatment with TAF9 siRNA significantly reduced GCSF-induced TP53 and TRIAP1 expression by 2.4-fold and 4.7-fold, respectively, in the rAION model. Overexpression of TAF9 significantly reduced apoptotic RGC and CASP3 levels and induced TP53 and TRIAP1 expression in the rAION model. ConclusionOur in vivo study is the first to report that GCSF is able to induce TAF9 expression to control RGC death and survival after ON infarct. Besides, the binding of TAF9 and TP53 is crucial to inhibit TP53 degradation and to modulate TRIAP1-CASP3 axis. Thus, we considered that TAF9 is a potential drug for patient with NAION.
Synthesis of SPIO nanoparticles and the subsequent applications in stem cell labeling for Parkinson’s disease
Authors: Yu Shuang , An Li , Tao Qing , Wu Yue , Wang Nana , Liu Yan , Wang Feifei , Zhang Lixing , Shi Aihua , Zhou Xiumin , Zhang Jingzhong 
Published: January 2021

Abstract
Parkinson's disease (PD) is characterized by the progressive loss of dopaminergic neurons in the midbrain, and the stem cell transplantation method provides a promising strategy for the treatment. In these studies, tracking the biological behaviors of the transplanted cells in vivo, is essential for a basic understanding of stem cell function and evaluation of clinical effectiveness. In the present study, we developed a novel ultras-small superparamagnetic iron oxide nanoparticles coating with the polyacrylic acid (PAA) and methoxypolyethylene glycol amine (PEG) by thermal decomposition method and a two-step modification. The USPIO-PAA/PEG NPs have a uniform diameter of 10.07 ± 0.55 nm and proper absorption peak of the corresponding ligands, as showed by TEM and FTIR. MTT showed that the survival of cells incubated with USPIO-PAA/PEG NPs remained above 96%. The synthesized USPIO-PAA/PEG had a good relaxation rate of 84.65 s-1 Mm-1, indicating that they could be efficiently uptake and traced by MRI. Furthermore, the primary human adipose-derived stem cells (HADSCs) were characterized, labeled with USPIO-PAA/PEG and transplanted into the striatum of 6-hydroxydopamine (6-OHDA) induced PD rat models. The labeled cells could be traced by MRI for up to 3 weeks after the transplantation surgery, moreover, transplantation with the labeled HADSCs significantly attenuated apomorphine-induced rotations in PD models, and increased the number of the dopaminergic neurons in the substania nigra. Overall, the development of USPIO-PAA/PEG NPs provides a promising tool for in vivo tracing technique of cell therapy, and identify a novel strategy to track stem cells with therapeutic potential in PD.
Fatigue Property of Additively Manufactured Ti-6Al-4V under Non-proportional Multiaxial Loading
Authors: Kimura Yuya , Ogawa Fumio , Itoh Takamoto 
Published: January 2021

Abstract
Low cycle fatigue strength properties of additively manufactured Ti-6Al-4V alloy were experimentally investigated under proportional and non-proportional multiaxial loading. Fatigue tests have been conducted by means of hollow cylinder specimens with and without heat treatments, at room temperature in air. Fatigue tests with proportional loading represented by a push-pull strain path and non-proportional loading represented by a circle strain path were conducted, respectively. The fatigue lives of additively manufactured specimens were drastically reduced obviously by internal voids and defects in comparison with the specimens used in the previous study [1]. In addition, the defect size is measured, and the defect does not cause fatigue strength reduction above some size. The fracture surface was observed using SEM to investigate fracture mechanism of additively manufactured specimens under two types of strain path. Different fracture patterns are recognized for the two strain paths; however, both showed the retention of the crack propagation in spite of the presence of numerous defects. The crack propagation properties of the materials with numerous defects under non-proportional multiaxial loading were elucidated to increase the reliability of additive manufactured components.
Novel Ring Compression Test Method to Determine the Stress-strain Relations and Mechanical Preperties of Metallic Materials
Authors: Han Guang-Zhao , Cai lixun , Bao Chen , Liang Bo , Lv Yang , Huang Mao-Bo , Liu Xiao-Kun 
Published: January 2021

Abstract
Although there are methods for testing the stress–strain relation and strength, which are the most fundamental and important properties of metallic materials, their application to small size specimens is limited. In this study, a new dimensionless elastoplastic load–displacement (EPLD-Ring) model for compressed metal rings with isotropy and constitutive power law is proposed to describe the relation between the geometric dimensions, Hollomon law parameters, load, and displacement based on energy density equivalence. Furthermore, a novel test method for the rings is developed to obtain the elastic modulus, stress–strain relation, yield strength, and tensile strength. The universality and accuracy of the model are verified within a wide range of imaginary materials via finite element analysis (FEA), and the results show that the stress–strain relations obtained with the model are more consistent with those inputted in the FEA software. Additionally, for seven metallic materials, a series of ring compression tests with various dimensions were performed. It was found that the stress–strain relations and mechanical properties predicted by the model are in agreement with the normal tensile test results. It is believed that the new method is reliable and effective for testing the mechanical properties of small size materials and tube components.
A Note on COVID-19 Diagnosis Number Prediction Model in China
Authors: Li Yi , Yin Xianhong , Liang Meng , Liu Xiaoyu , Hao Meng , Wang Yi 
Published: January 2021

Abstract
Objective: In December 2019, pneumonia infected with the novel coronavirus burst in Wuhan, China. We aimed to use a mathematical model to predict number of diagnosed patients in future to ease anxiety on the emergent situation. Methods: According to all diagnosis number from WHO website and combining with the transmission mode of infectious diseases, the mathematical model was fitted to predict future trend of outbreak. Our model was based on the epidemic situation in China, which could provide referential significance for disease prediction in other countries, and provide clues for prevention and intervention of relevant health authorities. In this retrospective, all diagnosis number from Jan 21 to Feb 10, 2020 reported from China was included and downloaded from WHO website. We develop a simple but accurate formula to predict the next day diagnosis number: ，where N i is the total diagnosed patient till the i th day, and was estimated as 0.904 at Feb 10. Results: Based on this model, it is predicted that the rate of disease infection will decrease exponentially. The total number of infected people is limited; thus, the disease will have limited impact. However, new diagnosis will last to end of March. Conclusions: Through the establishment of our model, we can better predict the trend of the epidemic in China.