bluuebunny/crossref_metadata_2025_split huggingface dataset:
Detection OF β- lactamase genes in clinical Klebsiella pneumonia strains isolated from hilla hospitals
Authors: Kadhim Kadher Marwa , Mohamme Jarallah Eman 
Published: October 2019

Abstract
This study aimed for isolation and identification of Klebsiella pneumonia, from clinical sample and from different hospitals in Hilla city. during the period from April to October 2017. 122/1056 (12%) isolates were belonged to Klebsiella pneumonia were collected. Growth on blood agar, MacConkey agar and Eosin methylene blue agar was identified by cultural, morphological and biochemical tests and confirmed by VITEK 2 system. The results showed that all the tested isolates were resistant to Ampicillin and Amoxicillin 122(100%), while 119(98%) for penicillin, whereas 100(82%) for piperacillin. Resistance to other drug classes varied among the isolates, a higher resistance was also detected with 95(78 %) to cefotaxime 99(81%) ceftazidime, 94(77%) to ceftriaxone and 92(75%) to ceftriaxone, The resistance to quinolones, nalidixic acid, ciprofloxacin and levofloxacin was detected 57(47%), 39(32%), 35(29%), respectively.majority of isolates 42 (34.4%) were obtained from sputum samples this result, 34 (27.8%) from urine, 17 (14 %) from stool, 15 (12.2 %) from burn, 8 ( 6.6 %) from vagina, 4 (3.3%) from wound, 1 (0.8%) from blood, 1 (0.8%) from ear and 0 (0 %) from both eye and throat. High prevalence rate for MDR, XDR and PDR in Merjan Teaching Hospital. The proportion of the patients from Merjan Teaching Hospital 27 (35.5%), 16 (44.4%) and 1 (50%) respectively. Detection of β - lactamase genes (blaCTX-M-9, blaCTX-M-10, and blaNDM-1) was performed by the conventional PCR technique. The results revealed that 36 isolates analyzed, blaCTX-M-9 12 (33.3%), blaCTX-M-10 14 (38.9%), and blaNDM-1 (0%).
Changes of gene expression profiles of infants inflammatory genes after Rota virus infection.
Authors: AL-Ghazal Abdulrhem T , Abdulghani Musto Mohammad 
Published: October 2019

Abstract
Rotavirus is a medical important pediatric pathogen causes severe gastroenteritis in infants, leading to 450,000 deaths globally each year. RT-PCR-Array technique analysis is a powerful method to determine global profiles of gene expression in cells and tissues after viral infections. Using this analysis, the Ct values of 14 immune inflammatory genes were subjected to determine their gene expression rates compared with Ct values of control (non-infected persons). Five housekeeping genes were used as internal control genes. All mRNA transcripts of these genes were upregulated (from 2.2974 fold change for CCL3, CCR1, CCR3, CXCL2, IFN-a, IFNA-g, IL1B, IL1R and 21.1121 fold change for TNF-b) after infection with rotavirus. This results revealed that rotavirus infection increase the gene expression rate of 14 inflammatory genes and lead to high impact on key immune pathways in infected infants.
Investigating Some Of Blood Parameters In Women With β-Thalassemia
Authors: AlAthari Rasha A , Mahdi Wijdan T 
Published: October 2019

AbstractThe current study was conducted in the Department of Life Sciences - Faculty of Science - University of Qadisiyah in cooperation with Maternity Hospital and Educational Children and the Center for Hematology in Diwaniyah for the period from 1/10/2017 to 1/4/2018. Fifty (50) samples were taken from women aged 15 to 35 years, and were divided into two groups: 30 women with thalassemia (experimental group) and 20 women without thalassemia (control group). Two (2) milliliter were taken from thalassemia-treated women and 2ml from healthy women for the purpose of conducting blood tests for phytoplankton, blood groups and the level of ferritin in the body. The results of the present study showed a significant decrease (P <0.01) in all blood parameters, hemoglobin, blood corpuscular, corpuscular volume, mean corpuscular hemoglobin in corpuscular and mean corpuscular hemoglobin contraction, compared with control group. Chemo-biological tests like ferritin testing confirmed significant ferritin increase by P<0.01 for the experimental group compared to the control group.
Histopathological study of Human Papillomavirus (HPV) in breast cancer patients
Authors: Al-Badry Bushra J , Talib Al-Muswie Rana , Jameel Sameera 
Published: October 2019

Abstract
A large number of epidemiological studies have evaluated the association between Human papillomavirus infection and breast carcinoma, but the results have been inconsistent.Numerous types of cytopathic effect are distinct in living cultures, but fixation and staining of the cells is essential to realize appearances of viral infection such as inclusion bodies. Identifying cytopathic effect and using it as a investigative tool needs much practice in observing stained section of numerous cell types. The present study aimed at diagnosing the histological changes in the malignant breast tissue, which caused by the HPV and comparing them with malignant breast tissue caused by other factors in order to find a way to diagnose the virus through the histopathic effect. A total of 100 samples was included in the study, 20 from benign tumor and 80 carcinomas, including different histopathological change. HPV was detected by using the conventional PCR. The breast cancer sections were examined for the expression of the effect of Human papillomavirus. In separate series the results of using formalin-fixed, paraffin -embedded sections were compared. Overall prevalence of HPV in malignant breast tissue was difference, we found that the histopathological characteristics of HPVassociated breast cancer are very different to breast cancercaused by other factors. Specifically, we showed the presence of HPV in breast cancer associates with the adenocarcinoma, The virus caused more activity in the tumor. Tumor is acinar carcinoma with increased cellularty and proliferative, While, in none infected with virus, there is no increased in cellularty or acinar, in some arease there was dilution of ducts.The observations indicate a likely causal role of HPV in causing a different histopathological change than breast cancercaused by other factors, This helps in the diagnosing of the HPV through cytopathic effect.
Study of some properties and antibacterial activity of (Alhagi maurorum Medik) collected from the desert of Al – Najaf
Authors: Abdulbary Meison 
Published: October 2019

Abstract
In traditional medicine, Alhagi maurorum is one member of medicinal plants which is used for many diseases therapy, that it is used as diuretic and Kidney stones treatments. This study was founded to identify some morphological characters and antibacterial activity of methanolic extract of A. maurorum. The morphological properties studied with macro and microscopical examination while the antibacterial activity by screening with well diffusion method against Bacillus cereus and Proteus mirabilis. The results showed that the plant is shrubby herb with evergreen appearance and its leaves are small obovate shape and the polygonal epidermal cells covered with thick cuticle layer. Stomata are frequently Paracytic type in addition to a few anomocytic type with unicellular covering trichome and prismatic crystals. The plant did not showed antibacterial activity against studied bacteria ( Bacillus cereus and Proteus mirabilis ) with concentrations up to 200 mg / ml.
Dissemination of 14bp deletion/insertion gene polymorphism of Human Leukocyte Antigen class I (G) with recurrent Spontaneous abortion in Baghdad
Authors: Jamal Abdulkhaliq Roua , Taher Mohammed Sabaa , Mohammed Abdul wahhab Alkhateeb Hiba , Abdul-Hassan Abbas Ahmed 
Published: October 2019

AbstractRecurrent spontaneous abortion (RSA) is a surprisingly common occurrence in various populations, risk factors associated with pregnancy losses are largely variable and often changes among different communities. This study was designed to determine the possible association between HLA-G 14bp insertion/deletion gene polymorphism with recurrent spontaneous abortion. Peripheral blood was collected from 210 women (180 women with recurrent abortion three or more abortions and 30 women with normal pregnancy to three or more birth and without any previous abortion) in the first trimester. Based on clinical examination and diagnostic laboratory findings of ELISA for TORCH test were selected ninety from 180 women with recurrent abortion in the current study were divided into three groups: group one included 30 women with recurrent abortion with sero-negative for TORCH test, group two also 30 women with recurrent abortion with ser-positive for anti-toxoplasmaantibodies, while control group included 30 women with a healthy pregnancy. In the current study not found any significant alteration between heterozygous and homozygous amongst three groups, also not found any implication between recurrent abortion and healthy pregnant in the field of alleles (+14bp insertion or -14bp deletion). The genotyping and alleles of HLA-G 14bp (insertion/deletion) were not give in to the hypothesis of connotation between HLA-G and recurrent spontaneous abortion.
Detection of Rhamnolipid Production in Pseudomonas aeruginosa
Authors: Shamaa Sarab al- , Bahjat Shababa 
Published: October 2019

Abstract
Large variety of microorganisms produce potent surface-active agents, biosurfactants, which vary in their chemical properties and molecular size. Rhamnolipids are naturally occurring glycolipids produced by Pseudomonas aeruginosa species of bacteria. The enormous diversity of biosurfactants makes them an interesting group of materials for application in many areas such as agriculture, public health, food, health care, waste utilization, and environmental pollution control such as in degradation of hydrocarbons present in soil. Their ability to reduce surface tension is a major characteristic of surfactants, which is the key ingredient used in detergents, shampoo, toothpastes. Four strains of Pseudomonas aeruginosa obtained from oil contaminated soil with diesel, kerosene or benzene using four detection methods: Blood hemolysis, Oil spreading technique, cetyl trimethylammonium bromide (CTAB) agar plate, and Emulsifying activity. Ten out of eleven strains tested demonstrated rhamnolipid production in one or more of the techniques used and with variable amounts, with Pseudomonas aeruginosa strain D and T displaying positive and high productivity in all methods used. A new modification of oil spread technique was performed in the present study as this method was found to be the most efficient, easiest, and reliable among the others.
Investigate of contamination of some wells with infectious stages of intestinal parasites in Saladdin - Iraq
Authors: Jamal Zangana Ashraf , Ahmad Erdeni Abid 
Published: October 2019

Abstract
The study investigated the pollution of water of some wells with infectious stages of intestinal parasites. Eleven water wells were examined. The results of the microscopic examination of water samples were cysts of, Entamoeba histolytica and Entamoeba coli and Toxocara canis and mature and immature eggs of Ascaris lumbricoides at different rates, as the results showed that the highest rate of E. histolytica is in the well number 10, which amounted cyst to 0.23 followed by cyst of E. coli in well No. 5, 0.11 and A. lumbircoides in wells 10 and 11 With 0.12 and 0.07 respectively, followed by I. canis eggs in both wells 9 and 2, 0.13 and 0.06 respectively, as well as physical and chemical tests of well-studied water. In this study, for the first time, the diagnosis of intestinal parasitic stages of infection in water wells, specifically in the province of Salah al-Din.
Prevalence study of tuberculosis among basrah population through 2011-2015 : prospective study
Authors: Edan Alsaimary Ihsan 
Published: October 2019

Abstract
the study recordes were taken from infectious diseases unite in albasrah general hospital. The data collected from the files of recorded cases thruogh 2011 to 2015. TB patients that attended to this unite which were, including pulmonary and extra pulmonary TB diseases. Their ages were between (few months-70 year). through 2011 the total patients reached to 58 males and 27 females. the highly risk group was aged 30-39yrs for males and 60-69 yrs for females. in 2012 the total patients reached to 52 males and 25 females. the highly risk group was aged 30-39yrs for males and females. in 2013 the total patients reached to 21 males and 24 females. the highly risk group was aged 30-39yrs for males and50-59 yrs for females. in 2014 the total patients reached to 19 males and 42 females. the highly risk group was aged 60-69 yrs for males and 20-29 yrs for females. And in 2015 the total patients reached to 31 males and 22 females. the highly risk group was aged 30-39 yrs for males and 30-39 and 50-59 yrs for females. There are statistical differences between all studied age groups through various years P<0.05
Role of outer membrane proteins in virulence of Klebsiella ozaenae and antibiotic sensitivity
Authors: Fahim Abbas Aalaa , Ghazi Mahdi Al-Saadi Aamal , Kadhim Hussein Anwar , Omran Al-Thaheb Azhar 
Published: October 2019

Abstract
In the present study, the association between outer membrane proteins loss, virulence and antibiotic sensitivity was investigated in K. ozaenae. An outer membrane proteins deficient K. ozaenae was derived from local clinical K. ozaenae isolate. Virulence was assessed by comparing the clearance of viable complete K. ozaenae with that outer membrane proteins deficient K. ozaenae in the spleen, liver, appendix, and blood of injected laboratory mice. Antibiotic susceptibility was tested by disc diffusion method. It was recorded significant differences at a P value of ⩽0.05 between the logarithm of the viable number of the complete K. ozaenae and logarithm of the viable number of outer membrane proteins deficient K. ozaenae that retrieved from the spleen, liver, appendix, and blood. It was observed that the logarithm of the viable number of complete K. ozaenae increased gradually during 18, 24, 48 and 72 hours compared with the logarithm of the viable number of outer membrane proteins deficient K. ozaenae which increases during 18-24 hours, and then decreased in all organs. Susceptibility tests showed that complete K. ozaenae isolate was resistant to most used antibiotics including amoxicillin, cephalothin, ceftazidime, cefotoxime, amikacin, tetracycline, naldixic acid, erythromycin, trimethoprim and sensitive to only imipenem and gentamicin. Whereas outer membrane deficient K. ozaenae isolate was resistant to all antibiotics used in our study.
Biological Synthesis of Silver Nanoparticles from saprolegnia parasitica
Authors: Ali Habeeb Al-Khuzai Rajaa , Khadhim Aboud Majid , Khoman Alwan Syoof 
Published: October 2019

Abstract
An eco-friendly method to synthesis silver nanoparticles using aquatic fungus Saprolegnia parasitica was investigated in current study. The formation of the silver nanoparticles (AgNPs) was initially confirmed by the UV-Visible Spectroscopy. As well as using FT-IR, SEM, and EDX. UV Visible analysis showed a strong peak at 428 nm, the SEM images of the biosynthesized AgNPs showed spherical shape with average of the nanoparticls diameter 37 nm range.
Therapeutic effect of P-coumaric acid in the experimentally infected rats with Salmonella typhi
Authors: Sadiq Obayes Maryam , Al-Bermani Oruba K , Ahmed Rahim Shaima 
Published: October 2019

AbstractSalmonella entericasubspeciesentericserotype typhi which infect the human and cause typhoid fevers (systemic infection). Experimental infection withSalmonella typhiare very important because they explains the pathogenicity ofSalmonella typhithrough a histopathological findings. The herbal inhibitors like P-Coumaric acid explain anti-inflammatory effect and have a selective effect against type III secretion system formation ofSalmonella typhi. our study includes 15 adult male albino rats were divided into three groups each group composed from 5 rats. A group: were orally challenged with 1ml of 3X106CFU ofSalmonella typhifor one day. Group B: were also orally administered with 1ml of 3X106CFU ofSalmonella typhi, additionally, they also orally received 5ml of 100mg/kg of P-Coumaric acid for three consecutive days. Group C: serving as control group, they orally administered with 5ml of normal saline. All the animals are sacrificed after five days post infection, liver, small intestine and colon specimens were taken for histopathological investigation. the histological sections of liver and small intestine of group A animals showed pathological changes like degeneration of hepatic cells and inflammatory cell infiltration of the lamina properia in small intestine, while the group B animals showed normal liver and small intestine sections like the control group
Cytotoxic activity of green synthesis copper oxide nanoparticles using cordia myxa L. aqueous extract on some breast cancer cell lines.
Authors: Ali Thamer Neran , Tareq Barakat Nadia 
Published: October 2019

Abstract
The efficiency of copper oxide nanoparticles synthesized from aqueous extract of cordia myxa L. on some breast cancer lines AMJ-13,MCF-7, and HBL-100 as normal cell lines was studied. Different concentration of copper oxide nanoparticles (25,50,75,100) µg/ml at different times (24,48 and72) h were selected. The results showed that the effect of nanoparticles depend on the concentration. As the concentration of nanoparticles increase the percent of inhibition increase. It was found that concentration of copper oxide nanoparticles at 100 μg / ml gave the highest inhibitory rate of cell growth MCF-7 (71.1%) after 24 hours, while the percent of inhibition for AMJ-13 was (69.6)%. When the exposure time was increased to48 h, the rate of inhibition at 100 mg/ml was 80% for MCF-7 while 73% for AMJ13. By increasing the duration of the exposure to 72 hours, the rate of inhibition at l00µg/ml were (85.2 and 78.2)for MCF-7 and AMJ13 respectively. No significant inhibition was found for HBL-100 at different concentration and different times. These results was indicated that copper oxide nanoparticles synthesized from the aqueous extract of cordia myxa L. had a toxic effect on growth of some breast cancer cell lines.
Antibiotics Susceptibility Pattern of Clinical and Environmental Escherichia coli Isolates from Babylon Hospitals
Authors: Hasan Abed Zainab , Mohammad Jarallah Eman 
Published: October 2019

Abstract
A total of (400) clinical and environmental samples were collected during the period from Sep/2018 to Jan/2019 from different hospitals in Hilla city. The isolates were identified and based on microscopic features and standard biochemical tests. The results showed there were (150) isolates of E.coli from clinical and the environmental samples. The clinical isolates (67 isolates) were collected from patients suffering from different infections such as UTI (30 isolates), gastroenteritis (diarrhea) (22 isolates), and wound infections (15 isolates). Also, the environmental isolates (83) were collected from the different site of hospitals environment include surgery room, hospital floors, the hospital cafeteria, the corridors of the hospital, and from workers dress and stool. All 150 E.coli isolates were primarily screened for colistin resistance, The following antibiotics were used for susceptibility testing to the resistance isolates for colistin (17 isolates): Aminoglycosides (Amikacin, Gentamicin, kanamycin ), Sulfonamide (Trimethoprim), Nitrofurans (Nitrofurantoin), Fluoroquinolons (Ciprofloxacin, Norfloxacin), Cefeme (Cephalothin, Ceftriaxone, Ceftazidime), Macrolides (Erythromycin), carbapenems (Imipenem, meropenem), beta-Lactams (Amoxicillin, Carbenicillin ), Tetracyclin, Chloramphenicol. our result show that E. coli the most causative agent of UTI infection especially in women. there is a high resistance to cefems and beta- lactam antibiotic (100%) among these isolates which play as a cell wall inhibitors. And moderate resistance to ciprofloxacin (52.9%), Chloramphenicol and Norfloxacin (29.14%), and weak resistance to Gentamycin.
The involvement of dityrosine crosslinks in lipofuscin accumulation in Alzheimer’s disease
Authors: Al-Hilaly Youssra K , Mohammed Asma H , Thorpe Julian R , Serpell Louise C 
Published: October 2019

AbstractLipofuscin is hydrophobic and insoluble yellow-brown pigment that accumulates in the nervous system of individuals and considered to be a biomarker of aging. However, it has been reported that amyloid-containing Alzheimer neurons contain a large amount of lipofuscin and lysosomal protease enzymes suggesting that the accumulation of Aβ may contribute to lipofuscin formation under oxidative stress conditions. Probing the contribution of oxidative stress using dityrosine cross-links as a marker will help to raise our understanding of the mechanism underlying the increased lipofuscin accumulation in Alzheimer (AD). In order to establish whether oxidised Aβ42 is found in lipofuscin pigments in AD brains, immunogold labelling for dityrosine and Aβ42 in lipofuscin of AD and control age matched brains was carried out. Single immunogold labelling of dityrosine was observed in the lipofuscin granules of control age-matched human brain. Interestingly, TEM immunogold labelling of dityrosine in lipofuscin of AD brain reveals two different labelling areas, low-and high-density dityrosine labelling. The quantification of immunogold particles shows significantly more dityrosine labelling in AD brain compared to age-matched controls. TEM immunogold co-labelling of dityrosine and Aβ in AD brain reveals some colocalisation within lipofuscin, although some areas showed low levels of Aβ labelling. These results may indicate that dityrosine cross-links could be generated from oxidation of various proteins that contain tyrosine residues. Our data show that dityrosine cross-links are increased in lipofuscin in AD brain, highlighting the important role played by dityrosine cross-links in the accumulation of lipofuscin in higher levels in AD compared to control brains.
Estimation of serum testosterone hormone according to anthropometric class in adult men
Authors: Hamid AL-Ardhi Ghadeer , Jameel Ibraheem Noran 
Published: October 2019

Abstract
Evaluate the serum concentration of testosterone hormone in some healthy adult’s men according to their characteristics of anthropometric measurements. This study included ninety-eight volunteer’s adult’s men, their ages (20-50) years, the blood samples were taken from subjects at the morning during 8:30-10:30 o’clock. ELISA kit was used to assay the serum level of testosterone hormone and the anthropometric measurements have been done such as; body mass index (BMI), waist circumference (WC) and percentage of body fat (BF%) by a special formula and the subjects were assorted depending on classes of anthropometric measurements. The results showed a significant decrease in serum level of testosterone hormone (p<0.05) in obese groups compared with the leaner groups. The class of the anthropometric measurements predicts the state of testosterone hormone level.
Saprolegnia parasitica–mediated biosynthesis of silver nanoparticles and its antimicrobial activity
Authors: Kadhim Al-Shibly Majid , Khoman Alwan Syoof , Ali Habeeb Rajaa 
Published: October 2019

Abstract
In this work, the effect of silver nanoparticles synthesized by Saprolegnia parasitica was investigated against.Staphylococcus.aureus, Proteus mirabilis, E.coli, Trichophyton.rubrum and Candida albicans, The formation of the silver nanoparticles (AgNPs) was initially confirmed by the UV-Visible Spectroscopy. As well as using SEM, UVVisible analysis showed a strong peak at 428 nm, the SEM images of the biosynthesized SNPs showed spherical shape with a particle size in the 37 nm range. The results of the study showed that the concentration of 150 microliters of SNPs solution was the most concentration for inhibition when it used as an antimicrobial against (E. coli, S.aureus, P.mirabilis, (T.rubrum).