bluuebunny/crossref_metadata_2025_split huggingface dataset:
Longer small bowel segments are resected in emergency surgery for ileocaecal Crohn’s disease with a higher ileostomy and complication rate
Authors: Celentano V. , O’Leary D. P. , Caiazzo A. , Flashman K. G. , Sagias F. , Conti J. , Senapati A. , Khan J. 
Published: October 2019

Abstract

Background
Repeated intestinal resections may have disabling consequences in patients with Crohn’s disease even in the absence of short bowel syndrome. Our aim was to evaluate the length of resected small bowel in patients undergoing elective and emergency surgery for ileocolic Crohn’s disease.

Methods
A prospective observational study was conducted on patients undergoing surgery for ileocolonic Crohn’s disease in a single colorectal centre from May 2010 to April 2018. The following patients were included: (1) patients with first presentation of ileocaecal Crohn’s disease undergoing elective surgery; (2) patients with ileocaecal Crohn’s disease undergoing emergency surgery; (3) patients with recurrent Crohn’s disease of the distal ileum undergoing elective surgery. The primary outcomes were length of resected small bowel and the ileostomy rate. Operating time, complications and readmissions within 30 days were the secondary outcomes.

Results
One hundred and sixty-eight patients were included: 87 patients in the elective primary surgery group, 50 patients in the emergency surgery group and 31 in the elective redo surgery group. Eleven patients (22%) in the emergency surgery group had an ileostomy compared to 10 (11.5%) in the elective surgery group (p < 0.0001). In the emergency surgery group the median length of the resected small bowel was 10 cm longer than into the group having elective surgery for primary Crohn’s disease.

Conclusions
Patients undergoing emergency surgery for Crohn’s disease have a higher rate of stoma formation and 30-day complications. Laparoscopic surgery in the emergency setting has a higher conversion rate and involves resection of longer segments of small bowel.
Cytotoxic effects of persistent organic pollutants on the freshwater snail (Lanistes carinatus) in Kafr El-Zayat, Egypt
Authors: Abdel-Halim K. Y. , Mona M. H. , Giesy J. P. , Shouker F. A. , Osman S. R. 
Published: October 2019

AbstractEffects of industrial and municipal wastewaters on the freshwater snail,Lanistes carinatus, were evaluated. Concentrations of some chemicals in some effluents were greater than permissible limits promulgated internationally by various jurisdictions. Pesticides and polychlorinated biphenyls (PCBs) observed in tissues of snails collected during summer were greater than those measured in snails collected during winter. Catalase activities observed during autumn were greater than those observed during other seasons. Activities of catalase were greater at all sites near sources of contamination than in snails from the reference site (S6). Lactate dehydrogenase activity was also greater at all sites relative to the location designated as the reference (S6), at which activities did not exceed 8.10 U/L. Patterns of genomic DNA in snails, as determined by use of OPA-02 primer, were significantly different among sites. Location S1 (Belshay village) exhibited 11 bands, followed by S2 (El-Demer zone) and S5 (Rosetta branch) which exhibited 6 bands. In contrast, all sites exhibited greater numbers of bands when the OPA-08 primer was used. Thus, DNA fingerprinting, lactate dehydrogenase, and catalase offer useful biomarkers in ecotoxicology and risk assessment programs.
Background seismicity and seismic monitoring in the Lai Chau reservoir area
Authors: Lizurek Grzegorz , Wiszniowski Jan , Giang N. V. , Van D. Q. , Dung L. V. , Tung V. D. , Plesiewicz Beata 
Published: October 2019

Abstract
Reservoir-triggered seismic activity depends not only on the technical characteristics of the future reservoir (filling volume, the height of water column) but also on the seismo-tectonics and the natural seismic processes occurring in the area before construction of an artificial reservoir. Passive seismic monitoring was realised near Lai Chau (Vietnam) before the impoundment started. It allowed exploration of the natural seismicity in the area of the future dam. Locations of seismic events several months prior to the reservoir impoundment were observed with ten stations installed in the reservoir vicinity. Events were mainly located near the dam along the Da river headwaters fault. However, only four stations were available for the entire period before the impoundment. Despite the network limitations, completeness of seismic catalogue and b value were determined and may be used as a baseline for analysis of the seismicity in this area after impoundment. The magnitude completeness level is significantly smaller than in the broader seismogenic zones covering an area of the dam. The b value differs from the results obtained for regional seismicity of Northeastern Vietnam. The capability of the local network for moment tensor inversion was estimated with the use of synthetic data tests. Test results provided the requirements for the station number according to azimuthal coverage of the network to obtain the reliable full moment tensor (MT) solution. Preliminary analysis of the seismic activity after Lai Chau reservoir impoundment indicates some changes in activity related to the impoundment and reservoir exploitation.
Pyridyl-Ala Modified Cyclic Hexapeptides: In-Vitro and In-Vivo Profiling for Oral Bioavailability
Authors: Vorherr Thomas , Lewis Ian , Berghausen Joerg , Huth Felix , Schaefer Michael , Wille Roman , Gao Jinhai , Wang Bing 
Published: October 2019

Abstract
We and others have been aiming at modifications to maintain or to enhance solubility while enabling permeability for cyclic hexapeptides. Especially, the 2-pyridyl-Ala modification was investigated, since in this case, the pyridyl-nitrogen is able to form an H-bond to the NH of the same residue. The hypothesis of a backbone side-chain interaction was demonstrated by NMR experiments, and further results obtained on a variety of pyridyl-Ala derivatives, studied systematically in the context of permeability, are presented in this contribution. Thus, this study sheds some more light on the pyridyl-Ala modification, which had been reported earlier. In addition to the in vitro profiling, the extent of oral bioavailability was assessed in rats. In principle, the pyridyl-Ala residue can be considered as an amino acid supporting oral uptake.

Graphic Abstract
Expression and analysis of zinc finger family gene in Lenzites gibbosa
Authors: Zhang Jun , Chi Yujie , Li Shuxuan , Zhang Jian , Chen Jie 
Published: October 2019

AbstractZinc finger transcription factors play significant roles in the growth and development of plant and animal, but their function remains obscure in fungi.Lenzites gibbosamycelia were extracted and sequenced by transcriptome analysis after growing on sawdust at different times to support mycelial growth ofL. gibbosain a nutrient matrix. Data bases used for analysis were the Kyoto encyclopedia of genes and genomes (KEGG) annotation, the cluster of orthologous groups of proteins (COG) and gene ontology (GO) annotation. Zinc finger class genes related to the growth and development ofL. gibbosawere screened. GO annotation and enrichment analysis of differentially expressed genes were carried out. A total of 114.55 Gb Clean Data were obtained from theL. gibbosatranscriptome. The average Clean Data in each sample was 6.16 Gb. The relative efficiency of reads between each sample and the reference genome was 88.5% to 91.4%. The COG analysis showed that most zinc finger protein genes were related to replication, recombination and repair function. GO enrichment analysis showed that the expressed genes involved in cellular process, cell part and binding. We identified seventy-two expressed genes including seven up-regulated genes and sixty-five down-regulated genes by applying DESeq2 data analysis software. By comparing the significantly expressed genes with KEGG database, 66 annotated sequences were obtained, and 35 primary metabolic pathways were annotated. Pathway enrichment analysis showed that differentially expressed genes were significantly enriched in protein processing in endoplasmic reticulum and ubiquitin-mediated proteolysis pathways. Gene_11750 and gene_5266 are highly correlated with the growth and development ofL. gibbosaand are closely related to protein processing in endoplasmic reticulum and ubiquitin-mediated proteolysis pathway. According to gene functional analysis, seven important differentially expressed genes related to the growth and development ofL. gibbosawere identified.
Molecular Heterogeneity of Papillary Thyroid Cancer: Comparison of Primary Tumors and Synchronous Metastases in Regional Lymph Nodes by Mass Spectrometry Imaging
Authors: Gawin Marta , Kurczyk Agata , Stobiecka Ewa , Frątczak Katarzyna , Polańska Joanna , Pietrowska Monika , Widłak Piotr 
Published: October 2019

Abstract
Intra-tumor heterogeneity results from both genetic heterogeneity of cancer (sub)clones and phenotypic plasticity of cancer cells that could be induced by different local microenvironments. Here, we used mass spectrometry imaging (MSI) to compare molecular profiles of primary tumors located in the thyroid gland and their synchronous metastases in regional lymph nodes to analyze phenotypic heterogeneity in papillary thyroid cancer. Two types of cancerous (primary tumor and metastasis) and two types of not cancerous (thyroid gland and lymph node) regions of interest (ROIs) were delineated in postoperative material from 11 patients, then the distribution of tryptic peptides (spectral components) was analyzed by MSI in all tissue regions. Moreover, tryptic peptides identified by shotgun proteomics in corresponding tissue lysates were matched to components detected by MSI to enable their hypothetical protein annotation. Unsupervised segmentation of all cancer ROIs revealed that different clusters dominated in tumor ROIs and metastasis ROIs. The intra-patient similarity between thyroid and tumor ROIs was higher than the intra-patient similarity between tumor and metastasis ROIs. Moreover, the similarity between tumor and its metastasis from the same patients was lower than similarities among tumors and among metastases from different patients (inter-patient similarity was higher for metastasis ROIs than for tumor ROIs). Components differentiating between tumor and its metastases were annotated as proteins involved in the organization of the cytoskeleton and chromatin, as well as proteins involved in immunity-related functions. We concluded that phenotypical heterogeneity between primary tumor and lymph node metastases from the same patient was higher than inter-tumor heterogeneity between primary tumors from different patients.
The Frontal Bone Window for Transcranial Doppler Ultrasonography in Critically Ill Patients: Validation of a New Approach in the ICU
Authors: Sentenac Pierre , Charbit Jonathan , Maury Camille , Bory Paul , Dagod Geoffrey , Greco Frédéric , Capdevila Xavier , Perrigault Pierre-François 
Published: October 2019

AbstractBackground and ObjectiveThe temporal bone window (TBW) for transcranial Doppler (TCD) often fails to insonate the anterior cerebral artery (ACA). The frontal bone window (FBW) has never been evaluated in intensive care units (ICU). The main objective was to determine the ability of the FBW to assess ACA velocities in critically ill patients.MethodsA prospective study was conducted in two ICUs of the Montpellier University Hospital (France), between November 2014 and September 2016. Adult patients admitted to ICU for brain injury, with a Glasgow Coma Scale score ≤ 13, were enrolled within 3 days after admission. A first TCD examination was carried out bilaterally through the TBW and FBW by an intensivist expert in TCD, repeated by the same examiner, and 15 min later by an intensivist certified in TCD, designated as non-expert, blinded. The success of the FBW examinations was defined by the ability to measure the ACA velocities. Intra- and interobserver agreements were analyzed according to the Bland and Altman method.ResultsA total of 147 patients were analyzed. The FBW succeeded in insonating the ACA in 66 patients [45%, CI (37–53)], 45 bilaterally and 21 unilaterally. For 16 patients (11%), the FBW was the only way to measure ACA velocities. By combining the two techniques, the ACA success rate increased from 62% CI (54–70) to 73% CI (65–79) (P = 0.05). Intra- and interobserver mean biases and 95% limits of agreement for ACA systolic velocity measurements through the FBW were 1 (− 33 to 35) and 2 (− 34 to 38) cm s−1, respectively. For paired TBW and FBW measures of ACA velocities, mean biases (± SD) for ACA systolic, and mean and diastolic velocities were relatively close to zero, but negatives (− 7 ± 33, − 2 ± 19, − 1 ± 15 cm s−1, respectively), highlighting that ACA velocities were lower with the FBW (A2 segment) than TBW (A1 segment). The correlation coefficient for ACA systolic velocities measured by the FBW and TBW wasR = 0.47, CI (0.28–0.62). No risk factors for failure of the FBW were identified.ConclusionsIn ICU, the FBW was able to insonate the ACA in 45% of patients admitted for brain injury, without the use of contrast agents. The FBW could improve the detection of ACA vasospasms.
Familial Alzheimer’s Disease and Recessive Modifiers
Authors: Vélez Jorge I. , Lopera Francisco , Silva Claudia T. , Villegas Andrés , Espinosa Lady G. , Vidal Oscar M. , Mastronardi Claudio A. , Arcos-Burgos Mauricio 
Published: October 2019

AbstractAlzheimer’s disease (AD) is progressive brain disorder that affects ~ 50 million people worldwide and has no current effective treatment. AD age of onset (ADAOO) has shown to be critical for the identification of genes that modify the appearance of AD signs and symptoms in a specific population. We clinically characterized and whole-exome genotyped 71 individuals with AD from thePaisagenetic isolate, segregating the (PSEN1) E280A dominant fully penetrant mutation, and analyzed the potential recessive effects of ~ 50,000 common functional genomic variants to the ADAOO. Standard quality control and filtering procedures were applied, and recessive single- and multi-locus linear mixed-effects models were used. We identified genetic variants in theSLC9C1,CSN1S1, andLOXL4acting recessively to delay ADAOO up to ~ 11, ~ 6, and ~ 9 years on average, respectively. In contrast, the CC recessive genotype in markerDHRS4L2-rs2273946 accelerates ADAOO by ~ 8 years. This study, reports new recessive variants modifying ADAOO inPSEN1E280A mutation carriers. This set of genes are implicated in important biological processes and molecular functions commonly affected by genes associated with the etiology of AD such asAPP,APOE, andCLU. Future functional studies using modern techniques such as induced pluripotent stem cells will allow a better understanding of the over expression and down regulation of these recessive modifier variants and hence the pathogenesis of AD. These results are important for prediction of AD and ultimately, substantial to develop new therapeutic strategies for individuals at risk or affected by AD.
Post-mining Remnants and Revitalization
Authors: Kaźmierczak Urszula , Strzałkowski Paweł , Lorenc Marek W. , Szumska Elżbieta , Sánchez Antonio Angel Peréz , Baker Kevin A. C. 
Published: October 2019

Abstract
The historic post-mining objects deserve special attention due to their high cognitive and didactic value. Conducting the revitalization of such facilities is aimed at preserving the mining cultural heritage, and as a result, it will insert attractiveness to the region. The publication attempts to present an approach to the issues of revitalization of post-mining objects and their analysis in Poland, Spain, and UK. In Poland, there is the Revitalization Act, which comprehensively defines stages and ways of conducting revitalization. Spanish legal regulations do not provide for a separate legal act on revitalization, but they are based on mining Law, environmental law, and cultural heritage law. On the other hand, legal regulations in UK do not provide solutions for the revitalization of post-mining areas. However, land leases could incorporate within them, prior to any industrial or mining activities commencement, a requirement for re-stabilization or returning the land to a safe environmental condition on activity completion. Despite the considerable diversity of legal conditions in the described countries, revitalization measures are conducted with positive results, as illustrated by the Gold Mine in Zloty Stok, La Tortilla Mine in Linares, and revitalization of King Edward Mine, an old mining site in Cornwall.
Secretor Status is Associated with Susceptibility to Disease in a Large GII.6 Norovirus Foodborne Outbreak
Authors: Sharma Sumit , Hagbom Marie , Carlsson Beatrice , Nederby Öhd Joanna , Insulander Mona , Eriksson Ronnie , Simonsson Magnus , Widerström Micael , Nordgren Johan 
Published: October 2019

Abstract
Norovirus is commonly associated with food and waterborne outbreaks. Genetic susceptibility to norovirus is largely dependent on presence of histo-blood group antigens (HBGA), specifically ABO, secretor, and Lewis phenotypes. The aim of the study was to determine the association between HBGAs to norovirus susceptibility during a large norovirus foodborne outbreak linked to genotype GII.6 in an office-based company in Stockholm, Sweden, 2015. A two-episode outbreak with symptoms of diarrhea and vomiting occurred in 2015. An online questionnaire was sent to all 1109 employees that had worked during the first outbreak episode. Food and water samples were collected from in-house restaurant and tested for bacterial and viral pathogens. In addition, fecal samples were collected from 8 employees that had diarrhea. To investigate genetic susceptibility during the outbreak, 98 saliva samples were analyzed for ABO, secretor, and Lewis phenotypes using ELISA. A total of 542 of 1109 (49%) employees reported gastrointestinal symptoms. All 8 fecal samples tested positive for GII norovirus, which was also detected in coleslaw collected from the in-house restaurant. Eating at the in-house restaurant was significantly associated with risk of symptom development. Nucleotide sequencing was successful for 5/8 fecal samples and all belonged to the GII.6 genotype. HBGA characterization showed a strong secretor association to norovirus-related symptoms (P = 0.014). No association between norovirus disease and ABO phenotypes was observed. The result of this study shows that non-secretors were significantly less likely to report symptoms in a large foodborne outbreak linked to the emerging GII.6 norovirus strain.
Who Is a Pathologist According to Oncology Patients and Internet Users? A Survey Study
Authors: Kunc Michał , Miszewski Kevin , Bieńkowski Michał , Kamieniecki Alexandra , Ekman Marcin , Gorczyński Adam , Biernat Wojciech 
Published: October 2019

AbstractThe pathologist is frequently called “the doctor’s doctor.” However, there are many uncertainties about the role of a pathologist among patients and policymakers and even among other medical specialties. The aim of the current study is to analyze the misconceptions of who a pathologist is among inpatients and Internet users, to find where the lack of understanding is originating from, and to confirm the need to educate the general public about pathologists. The survey of Internet users was conducted among Facebook users, utilizing the snowball sampling method. Inpatients were randomly recruited in the Department of Surgical Oncology. Seventy-eight inpatients and 320 Internet users were enrolled in the study. Significantly, more hospital patients than Internet users answered that the pathologist is not an MD (p = 0.00953). A portion of participants stated that pathologists do not make diagnoses (n = 28, 7.03%) and do not influence the treatment plan (n = 37, 9.30%) and that the other specialists do not gain anything from the pathologist’s work (n = 67, 16.83%). Only 15.07% of respondents had their information about pathologists from other doctors. The findings from this study should show that even the most basic knowledge of a pathologist being an MD is not known. Pathologists are not recognized for being involved in the diagnosis of diseases. This should provide an incentive to pathologists to teach future doctors, policymakers, and patients about the perplexity of the pathology specialty. It shows obvious gaps in the knowledge of the treatment process as a whole.
Tax efficiency in a model of endogenous markups
Authors: Durceylan Esra 
Published: October 2019

Abstract

Efficiency comparison of ad valorem and unit taxes has been traditionally based on consumer welfare. However, if the tax instrument also affects the distribution of firms over their productivities, the policy maker may be concerned about the implications on aggregate productivity as well. This paper makes an efficiency comparison of ad valorem and unit taxes by allowing the distribution of firms to respond to changes in policy. First, I make an efficiency comparison in a model with monopolistically competitive firms that are homogenous with respect to their productivity levels. Consumer preferences exhibit love for variety and allow firms to adjust their markups. I find that ad valorem tax is more efficient. Allowing for firm heterogeneity overturns this result at high revenue requirements. As the tax rate increases, ad valorem tax causes excessive exit of firms which makes the market more competitive. Hence, few surviving firms price lower by decreasing their markups. Lower prices decrease the tax revenue collected. As a result under ad valorem tax regime, higher consumer surplus is dominated by lower tax revenue. On the other hand, production is concentrated among relatively more productive firms. Thus, aggregate productivity is higher under ad valorem tax regime.
Evaluation of Nile tilapia in monoculture and polyculture with giant freshwater prawn in biofloc technology system and in recirculation aquaculture system
Authors: Hisano Hamilton , Barbosa Phillipe T. L. , Hayd Liliam A. , Mattioli Cristiano C. 
Published: October 2019

Abstract
Biofloc technology system (BFT), recirculation aquaculture system (RAS) and polyculture promote efficient use of water, area and nutrient recycling, which are essential practices for sustainable aquaculture development. The aim of this study was to evaluate the growth, feed efficiency, biofloc composition and water quality of Nile tilapia Oreochromis niloticus (Linnaeus, 1758) in monoculture and polyculture with giant freshwater prawn Macrobrachium rosenbergii (De Man, 1906) in BFT and RAS, over a period of 30 days. Fish (n = 128; 7.29 ± 0.67 g) were distributed randomly in 16 experimental tanks (8 fish/tank). Prawn (n = 96; 0.50 ± 0.09 g) were allocated in 8 experimental tanks (12 prawn/tank) in a polyculture. The experimental design was completely randomized with four treatments with four replicates each, in a factorial design 2 × 2 (BFT and RAS vs. monoculture and polyculture). The experimental diet (28% of digestible protein; 3100 kcal kg−1 of digestible energy) was used both to fish and prawn in BFT and RAS. There was significant effect (p < 0.01) of the system and the culture for weight gain, apparent feed conversion and protein efficiency ratio. The average weight gain and apparent feed conversion of tilapia in monoculture (30.04 g and 1.39) and in polyculture (36.44 g and 1.27) were superior (p < 0.01) in BFT than in monoculture (23.64 g and 1.74) and in polyculture (24.14 g and 1.61) in RAS. Weight gain and survival of giant freshwater prawn was superior (p < 0.01) in BFT (0.43 g and 87%) compared to RAS (0.26 g and 79%). The data showed that BFT provides better growth performance responses in monoculture for Nile tilapia and in polyculture with giant freshwater prawn compared to RAS.