bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ischemic stroke with a preceding Trans ischemic attack(TIA) less than 24 hours and thrombolytic therapy
Authors: Poupore Nicolas , Strat Dan , Mackey Tristan , Snell Ashley , Nathaniel Thomas 
Published: March 2020

Abstract
Background Acute ischemic stroke attack with and without a recent TIA within or less than 24 hours may differ in clinical risk factors, and this may affect treatment outcomes following thrombolytic therapy. We examined whether the odds of exclusion or inclusion for thrombolytic therapy are greater in ischemic stroke with TIA less than 24 hours preceding ischemic stroke(TIA-24hr-ischemic stroke patients) as compared to those without recent TIA or non-TIA <24 hours. Methods A retrospective hospital-based analysis was conducted on 6,315 ischemic stroke patients, of whom 846 had proven brain diffusion-weighted magnetic resonance imaging (DW-MRI) of an antecedent TIA within 24 hours prior to ischemic stroke. The logistic regression model was developed to generate odds ratios (OR) to determine clinical factors that may increase the likelihood of exclusion or inclusion for thrombolytic therapy. The validity of the model was tested using a Hosmer-Lemeshow test, while the Receiver Operating Curve (ROC) was used to test the sensitivity of our model. Results In TIA-24hr-ischemic stroke population, patients with a history of alcohol abuse (OR = 5.525, 95% CI, 1.003-30.434, p = 0.05), migraine (OR=4.277, 95% CI, 1.095-16.703, p=0.037), and increasing NIHSS score (OR=1.156, 95% CI, 1.058-1.263, p = 0.001) were associated with the increasing odds of receiving rtPA, while older patients (OR = 0.965, 95% CI, 0.934‐0.997, P = 0.033) were associated with the increasing odds of not receiving rtPA. Conclusion In TIA-24hr-ischemic stroke patients, older patients with higher INR values are associated with increasing odds of exclusion from thrombolytic therapy. Our findings demonstrate clinical risks factors that can be targeted to improve the use and eligibility for rtPA in in TIA-24hr-ischemic stroke patients.
Performance, metabolic and hormonal responses of grazing Nellore cows to an energy-protein supplementation during the pre-partum phase
Authors: Ferreira Matheus Fellipe de Lana , Rennó Luciana Navajas , Detmann Edenio , Paulino Mário Fonseca , Filho Sebastião de Campos Valadares , Moreira Samira Silveira , Martins Hudson Caio , de Oliveira Bruno Inácio Correa , Marquez Julia Avansi , Cidrine Isabela de Paula 
Published: March 2020

Abstract

Background: Supplementation programs for prepartum beef cows are usually adopted because the nutritional status of the cows upon calving is a main factor impacting reproductive performance of the next production cycle. This study evaluated the effects of 60-d pre-partum energy-protein supplementation on performance, metabolic and hormonal responses during the peripartum phase of grazing beef cows. Thirty-eight pregnant multiparous Nellore cows were assigned to a completely randomized design with two treatments: control (no supplement) and supplementation (1.5 kg of energy-protein per d with 30% crude protein, dry matter basis) Results: The supplemented cows had higher ADG pre-partum (P<0.10), but postpartum ADG did not differ between treatments. Supplementation did not affect BCS and calf BW upon calving, on days 45 and 90, milk yield and composition (P>0.10). No differences were found for forage intake and neutral detergent fiber digestibility (P>0.10). The intake and digestibility of CP and OM increased in response to supplementation (P<0.10). An interaction occurred between supplementation and peripartum days for BUN, βHB, T3 and T4, which had higher concentrations for supplemented cows at pre-partum period (P<0.10). Concentration of others blood parameters significantly changed along peripartum days (P<0.10). There was no difference in pregnancy rates and days from calving to conception between treatments (P>0.10). Conclusions: Providing an energy and protein supplement to grazing Nellore cows over the last 60 d of gestation improved their pre-partum energy balance. However, no post-partum carryover effects were detected. Keywords: nutrition, metabolism, parturition, Zebu
Molecular epidemiology of drug resistant Mycobacterium tuberculosis in Africa: a systematic review
Authors: Chisompola Namaunga Kasumu , Streicher Elizabeth Maria , Muchemwa Chishala Miriam Kapambwe , Warren Robin Mark , Sampson Samantha Leigh 
Published: March 2020

Abstract
Background The burden of drug resistant tuberculosis in Africa is largely driven by the emergence and spread of multidrug resistant (MDR) and extensively drug resistant (XDR) Mycobacterium tuberculosis strains. MDR-TB is defined as resistance to isoniazid and rifampicin, while XDR-TB is defined as MDR-TB with added resistance to any of the second line injectable drugs and any fluoroquinolone. The highest burden of drug resistant TB is seen in countries further experiencing an HIV epidemic. The molecular mechanisms of drug resistance as well as the evolution of drug resistant TB strains have been widely studied using various genotyping tools. The study aimed to analyse the drug resistant lineages in circulation and the transmission dynamics of these lineages in Africa by describing outbreaks, nosocomial transmission and migration. Viewed as a whole, this can give a better insight into the transmission dynamics of drug resistant TB in Africa. Methods A systematic review was performed on peer reviewed original research extracted from PubMed reporting on the lineages associated with drug resistant TB from African countries, and their association with outbreaks, nosocomial transmission and migration. The search terms “Tuberculosis AND drug resistance AND Africa AND (spoligotyping OR molecular epidemiology OR IS6110 OR MIRU OR DNA fingerprinting OR RFLP OR VNTR OR WGS)” were used to identify relevant articles reporting the molecular epidemiology of drug resistant TB in Africa. Results Diverse genotypes are associated with drug resistant TB in Africa, with variations in strain predominance within the continent. Lineage 4 predominates across Africa demonstrating the ability of these “modern strains” to adapt and spread easily. Most studies under review reported primary drug resistance as the predominant type of transmission. Drug resistant TB strains are associated with community and nosocomial outbreaks involving MDR- and XDR-TB strains. The under-use of molecular epidemiological tools is of concern, resulting in gaps in knowledge of the transmission dynamics of drug resistant TB on the continent.Conclusions Genetic diversity of M. tuberculosis strains has been demonstrated across Africa implying that diverse genotypes are driving the epidemiology of drug resistant TB across the continent.
Oxidative stress and gut-derived lipopolysaccharides in children affected by Paediatric Autoimmune Neuropsychiatric Disorders Associated with Streptococcal infections.
Authors: Loffredo Lorenzo , Spalice Alberto , Salvatori Francesca , Giovanna De Castro , Guido Cristiana Alessia , Zicari Anna Maria , Ciacci Paolo , Battaglia Simona , Brindisi Giulia , Ettorre Evaristo , Nocella Cristina , Salvatori Guglielmo , Duse Marzia , Violi Francesco , Carnevale Roberto 
Published: March 2020

Abstract
Background: paediatric autoimmune neuropsychiatric disorders associated with streptococcal infections syndrome (PANDAS) identifies patients with acute onset of obsessive-compulsive and tic disorders. The objective of this study was to evaluate serum NOX2 levels, as well as 8-iso-prostaglandin F2α (8-iso-PGF2α) and lipopolysaccharide (LPS) of PANDAS patients. Methods: in this study we wanted to compare serum levels of soluble NOX2-dp (sNOX-2-dp), iso-PGF2α and LPS in 60 consecutive subjects, including 30 children affected by PANDAS and 30 controls (CT) matched for age and gender. Serum zonulin was used as intestinal permeability assay.Results: compared with CT, PANDAS children had increased serum levels of sNOX-2-dp, 8-iso-PGF2α and LPS. Bivariate analysis showed that serum sNOX2-dp was signiﬁcantly correlated with LPS (Rs=0.359; p=0.005), zonulin (Rs=0.444; p<0.001) and 8-iso-PGF2α (Rs=0.704; p<0.001). Serum LPS signiﬁcantly correlated with zonulin (Rs=0.610; p<0.001), and 8-iso-PGF2α (Rs=0.591; p=0.001). Finally, a multiple linear regression analysis showed that serum 8-iso-PGF2α and zonulin were the only independent variables associated with sNOX2-dp (R2=68%).Conclusion: this study shows that children affected by PANDAS have high circulating levels of sNOX2-dp, isoprostanes and of LPS that could be involved in the process of neuroinflammation.
Preparation and bioactivity of anti-Newcastle disease virus-phosphoprotein cytoplasmic transduction peptide antibody
Authors: Li Benqiang , Wang Man , Zhu Jianguo 
Published: March 2020

Abstract
On the basis of cell-penetrating peptide’s character that it can penetrate cytomembrane and transfer macromolecular protein to cytoplasm so to play biological function, we took the experiments. The fuse penetrating peptide our experiment adoptted is HIV-TAT derived fragment-CTP512, with good transmember effect and distinct cytoplasm-position. In this chapter, the research of transmembrane character was processed first. According to the tests on trans- member protein with different concentrations, the best trans-member concentration is 3µM. Afterwards, we found that the location of trans-member antibody is overlapping with phosphoprotein using indirect immunofluorescence test analysis. According to MTT test, there is no significant difference between CTP fusion protein and control on cell proliferation and viability. TCID50 test was used to detect the protective effect of trans-member antibody on cell. Result showed that trans- member antibody has significant cell protection effect compared to the control in the order: ZL.103>ZL.17>Control. Fluorogenic quantitative PCR result showed that trans- member antibody can disturb the duplication and transcription of Newcastle disease virus. This results not only paved a good way to research the transport of disease related protein, but also provide a splendid tool on protein function research.
Perspectives of health care providers on the role of culture in the self-care of patients with chronic heart failure: a qualitative interview study conducted in Israel
Authors: Joensson Adam , Cewers Emilie , Weinstein Jean Marc , Gal Tuvia Ben , Stromberg Anna , Jaarsma Tiny 
Published: March 2020

Abstract
Background: Self-care is recognized as important behaviour in chronic diseases such as heart failure (HF). The cultural background of patients with HF is one of the factors that can be considered to affect their adherence to self-care. The cultural background of the health care providers might also influence their view on self-care behaviour and the education they provide. The aim of this study was to describe health care providers' perceptions of the role of culture in self-care and how those perceptions shape their experiences and their practices.Methods: A qualitative study was performed in Israel, a country with a culturally diverse population. Data was collected using semi-structured interviews with 12 healthcare providers from different cultural backgrounds, selected by purposeful sampling, from two hospitals in Israel. Interviews were audio recorded and transcribed verbatim. Data was analysed using content analysis.Results: Healthcare providers experienced cultural background influenced their patients’ self-care behaviour. Perceived cultural-specific barriers to self-care such as: dietary traditions interfering with the recommended HF diet, willingness to undertake self-care and beliefs conflicting with medical treatment were identified. Healthcare providers described that they adapted their patient education and care based on the cultural background of the patients. Shared cultural background, awareness and knowledge of differences were described as positively influencing self-care education, while cultural differences could complicate this process. These findings are encapsulated within four categories regarding perceptions of health care providers: ‘Culture permeates self-care behaviours’, ‘Culture influence the way care is provided’, ‘Mutual cultural background impacts the mindset to address self-care’ and ‘Culture is only a small piece of the puzzle...’Conclusions: Cultural-specific barriers for self-care were perceived by health care providers and they identified that their own cultural background shapes their experiences and their practices.
A giant hepatic cavernous hemangioma with an abdominal wall hemangioma: a case report
Authors: Liu Yan , Gu Xue , Zhou Zhang , Yang Na , Luo Cheng-jun , Li Li 
Published: March 2020

Abstract
Introdoction: Hepatic cavernous hemangioma is the most common benign tumor of the liver without special treatment，which is very common in clinical work.But some larger cavernous hemangioma has the risk of bleeding, clinical diagnosis and treatment of large hepatic cavernous hemangioma is of great significance.Case presentation: The patient was a 45-year-old middle-aged woman who was admitted to the hospital due to a large lesion of the abdominal wall. At first, she was considered as having a melanoma of the abdominal wall. After imaging and pathological examinations, she was diagnosed as having a giant cavernous hemangioma of the liver. Percutaneous hepatic artery superselective angiography and embolization significantly reduced the lesions.Conclusion: Hepatic cavernous hemangioma is relatively more common clinically, but there are few reports of multiple and huge hemangioma, as reported here, our patient had a large abdominal wall hemangioma. It is speculated that the coexistence of the two is related. In fact,during embryonic development,the fetus and mother are connected by two umbilical arteries and one umbilical vein. An aneurysm-like deformity caused by excessive development or abnormal differentiation of blood vessels during the developmental process may be the cause of the patient's massive abdominal wall and liver hemangiomas.
Trends in Research Related to Premenstrual Syndrome and Premenstrual Dysphoric Disorder: a Bibliometric Analysis
Authors: Gao Mingzhou , SUN Hui , ZHANG Changlong , GAO Dongmei , QIAO Mingqi 
Published: March 2020

Abstract
Background The global incidence of premenstrual syndrome (PMS) and premenstrual dysphoric disorder (PMDD) is increasing, with increasing suicide reports. However, the bibliometric analysis of global research on PMS and PMDD is rare. We aimed to evaluate the global scientific output of research on PMS and PMDD and to explore their research hotspots and frontiers from 1945 to 2018 using a bibliometric analysis methodology.Methods Articles with research on PMS and PMDD between 1945 and 2018 were retrieved from the Web of Science Core Collection (WoSCC). We used the bibliometric method, CiteSpace V and VOSviewer to analyze publication years, journals, countries, institutions, authors, research hotspots, and trends. We plotted the reference co-citation network, and we used keywords to analyze the research hot spots and trends.Results We identified 2,833 publications on PMS and PMDD research from 1945 to 2018, and the annual publication number increased with time, with fluctuations. Psychoneuroendocrinology published the highest number of articles. The United States ranked the highest among the countries with the most publications, and the leading institute was UNIV PENN. Keyword and reference analysis indicated that the menstrual cycle, depression and ovarian hormones were the research hotspots, whereas prevalence, systematic review, anxiety and depression and young women were the research frontiers.Conclusions We depicted overall research on PMS and PMDD by a bibliometric analysis methodology. Prevalence and impact in young women , systematic review evaluations of risk factors, and the association of anxiety and depression with menstrual cycle phases are the latest research frontiers that will pioneer the direction of research in the next few years.
The Effect of Mobile Instant Messaging versus Face-to-Face Counseling on the Primiparous Mothers’ Breastfeeding Self-efficacy: A Pilot Randomized Controlled Trial
Authors: Mehrabi Manoosh , Zarei Shoale , Bazrafkan Leila , Safarpour Ali Reza 
Published: March 2020

Abstract
Background Increasing breastfeeding rates around the world is one of the most important goals of the World Health Organization. Self-efficacy is a modifying and predictive factor for initiation and continuation of breastfeeding. This study was conducted to investigate the impact of mobile-based education and regular delivery of designed messages on breastfeeding self-efficacy in primiparous mothers. Methods This study was a double blind pilot randomized controlled trial, in which a hundred and twenty primiparous breastfeeding mothers were randomly allocated into two groups using permuted block randomization. The standard method conseling arm received routine counseling interventions and the intervention arm received a mobile instant messaging program in addition to the usual counseling. The main objective of this study was to compare self-efficacy in face-to-face counseling group and mobile instant messaging group. Self-efficacy levels were compared in the two groups before and after the study procedures. Results In this study, 60 mothers were studied in each group, and then the collected data were analyzed. The mean post-test scores in the intervention group (60.40 ± 4.92) and the control group (50.10 ± 7.60) were compared in the main analysis. The results indicated a statistically significant difference (p <0.001). Given the amount of effect size ( d= 0.99; 95% CI=1.19-2.02 ) it appears that there is a high level correlation between the applied intervention and level of self-efficacy among the study participants, especially those with higher levels of education. Secondary findings of the study involved evaluating the effects of education, occupation, family income, lactation duration and spouse support for breastfeeding self-efficacy. Except for the maternal education level, which had a significant relationship with the level of breastfeeding self-efficacy (p= 0.02), the other factors did not show any correlation with self-efficacy in breastfeeding. Conclusion The pilot study provided valuable information for feasibility assessment of randomized controlled trials in future studies with larger sample sizes and more participant diversity.
The Effect of Parents ‘web-based training on the Level of Post-Traumatic Stress Symptoms in Children: A Quasi-Experimental Study
Authors: Ashkalak Zakieh Omidvar , Nazari Hadis , Seyedfatemi Naima , Haghani Hamid , Parvizy Soroor 
Published: March 2020

Abstract
Background Post-traumatic stress disorder is a common occurrence in children that can affect their overall physical and mental functioning and quality of life. Early intervention can have significant health benefits for children. The purpose of this study was to investigate the effects of parents’ web-based training on the level of post-traumatic stress symptoms in children. Methods This study was a quasi-experimental one with two groups that is intervention and control group. 110 children aged 10 to 18 with traumatic stress symptoms and one of their parents were selected through available sampling. Data were collected by a researcher-made demographic questionnaire and the Child Revised Impact of Events Scale (CRIES-8). The control group received routine care, but the experimental group received a 4-week training course through a researcher-designed website. Two weeks after the intervention, the child stress level was measured and compared within both groups. Data were analyzed using SPSS V.22. Results The difference between the mean score of total traumatic stress and its subscales before intervention was not statistically significant (P=0.23). After intervention, however, the mean score of total traumatic stress and its subscales decreased in the experimental group and increased in the control group and the difference was statistically significant (P<0.001). Conclusions E-learning intervention by parents as a nursing intervention reduces the level of traumatic stress symptoms in children. This easy task of web-based training can be recommended to reduce the symptoms of traumatic stress and possibly to increase recovery in these patients.
Analysis of the electrophoretic profiles of prion protein in carcinous and pericarcinous lysates of six different types of cancers
Authors: Wei Wei , Shi Qi , Wu Yue-Zhang , Yang Xue-Hua , Xiao Kang , Dong Xiaoping 
Published: March 2020

Abstract
Introduction PrP (Prion Protein) is a cellular membrane protein distributing in many kinds of tissue cells. Besides its critical role in prion disease, the potential roles of cellular PrP in human malignant tumors have been also addressed. However, the profiles of PrP in malignant tumors, such as the patterns in electrophoresis and biochemistry features, are still not well known. Objective To find the different electrophoretic profiles of prion protein in carcinous and pericarcinous lysates of six different types of cancers. Methods Western Blot was used to test the amounts of total PrP in cancer samples and the tolerance of PK (protease K) digestion among various tissue types. Results A mass of the PrP signals with large molecular weight were identified in the homogenates of peripheral tissues. The amounts of total PrP evaluated by Western blots did not differ significantly between carcinous and pericarcinous tissues. PrPs in all types of the tested cancer samples were PK sensitive, but showed diversity in the tolerance of PK digestion among various tissue types. Conclusions The electrophoretic patterns of carcinous and pericarcinous tissues were almost similar, but markedly different from that of brain tissues.