bluuebunny/crossref_metadata_2025_split huggingface dataset:
Metastatic renal cell carcinoma patients of T4 stage who are in status of N1 stage or older than 76 years cannot benefit from Cytoreductive Nephrectomy
Authors: Zhang Zhao , Wu Hongliang , Yang Tong , Wu Yaohai , Yu Nengwang , Xu Zhonghua 
Published: August 2020

Abstract
Background: We aimed to identify which part of the patients with metastatic renal cell carcinoma (mRCC) is not suitable for cytoreductive nephrectomy (CN).Methods: The data of mRCC patients was acquired from the Surveillance, Epidemiology, and End Results (SEER) database. Multivariate cox regression analysis and nomogram were performed for selecting factors independently associated with survival. Propensity score matching (PSM) was applied to reduce potential bias when comparing survival of mRCC patients treated by CN or non-surgery (NS). The survival analysis of subgroups was estimated by the Kaplan–Meier method and compared by log-rank testing. The summary of subgroup analysis was showed by forest plots.Results: The records of 21411 patients with mRCC were obtained from the SEER database. After screening, a total of 6532 patients were included for further analysis, of which 6043 underwent CN and 489 underwent NS. Age, T stage, N stage and tumor size were involved in subgroup analysis by PSM according to the result of multivariate cox regression analysis and clinical experience. Survival benefit was not found in T4 stage patients. Further analysis showed that T4&N1 and T4&age≥76yr subgroups could not obtain survival benefit from CN.Conclusion: CN should not be performed in T4 stage mRCC patients who were in status of N1 stage or older than 76 years, because surgery cannot take significant survival benefit for them.
Mild Chronic Hypoxia-Induced HIF-2α Interacts with c-MYC Through Competition with HIF-1α in Hepatocellular Carcinoma Proliferation
Authors: Mu Han , Yu Ge , Li Huikai , Wang Mengmeng , Cui Yunlong , Zhang Ti , Song Tianqiang , Liu Changfu 
Published: August 2020

Abstract
The authors have withdrawn this preprint due to author disagreement.
Perception of Faculty and Students About E Learning About Feasibility, Acceptance and Implementation in Covid Crisis&nbsp;&nbsp;
Authors: Menon Pramila , Pande Vineeta , Jadhav Sudhir , Agarkhedkar Sharad 
Published: August 2020

Abstract
The study aimed to understand the perception, acceptance and feasibility of E learning for undergraduate medical students and faculty during the Carona pandemic and get suggestions to improve the teaching learning process. Methods: Total 447 (436+11) were approached for the response through google form. Out of 447 participants of 321 responses ( 71%; 95 CI 67.5-75.8 ) recorded and 302 (96%; 95 CI 90.9-96.4) gave consent which included (students 211 and 11 faculty) who experienced E learning as teaching learning method and their feedback collected using an semi structured questionnaire as google form in google classroom. Results: A majority 81%( 95 CI 67.5-75.8) had positive responses towards various aspects of the online classes. Google classroom and video based platforms like zoom,cisco webex, google meet were the most preferred and convenient platforms. Conclusion: Online classes were accepted by the most of the students and faculty. The positive feedback and suggestions helped in the planning of E learning classes in the existing curriculum for future.
Refractive Stability and Ocular Biometric Changes After Customized LASEK for Correction of Myopia; 8 Years Follow up
Authors: Hashemian Seyed Javad , Es'haghi Acieh , Aghaei Hossein , Ghiasian Leila , Jafari Mohammad Ebrahim , Hashemian Mahsa Sadat , Hashemian Seyed Mahyar , Janani Leila 
Published: August 2020

Abstract
BACKGROUND: To assess long-term Visual and refractive stability and ocular biometric changes in low to moderate myopic subjects treated by customized LASEK. METHODS: Seventy eyes of 35 patients were treated by customized LASEK for correction of less than 6 diopter myopia in this cohort study. Uncorrected visual acuities (UCVA) and distance corrected visual acuities (DCVA), keratometry values and ocular biometric data by Lenstar LS900 including anterior chamber depth (ACD), aqueous depth, central corneal thickness(CCT),lens thickness(LT) and axial length(AL) were evaluated pre and 8 years postoperatively. RESULTS: Mean pre-operative spherical equivalent was -3.99 ±1.38 diopter (D) that improved to 0.01 ±0.27 D and -0.10 ±0.31 D, 6 months and 8 years postoperative respectively. Mean pachymetry, flat, steep and mean keratometry values increased significantly from 6 months to 8 years postoperative. Although, these changes had no significant effect on visual outcomes and subjective refraction. ACD decreased and LT increased significantly over 8 years follow-up in comparison with preoperative values. The change in AL was not significant at 8 years follow up.CONCLUSIONS: The long-term visual and refractive outcomes of customized LASEK in correction of low to moderate myopia were stable and predictable, although changes in ocular biometric parameters have occurred.
Application of Ginkgo Biloba L. Extract at Early Stage of Tumor Development Helps Cyclophosphamide Inhibit the Growth of Tumor Cells
Authors: Chen Dawei , Yang Jinghui , Meng Fanzhu , Beseler Cheryl L. , Li Hao , Liu Xiaomei , Guo Yinpei , Qin Lili , Zuo Ruyue , Ren Shuping 
Published: August 2020

Abstract
Background There is inconsistency in the application of antioxidants in tumor treament, the study is to explore whether the application of an antioxidant at the early stage of tumor transplantation enhances efficacy of a chemotherapeutic agent in inhibiting tumor cell growth.Methods EMT-6 cells were injected into the 48 ICR mice. 24h later, cyclophosphamide (CTX) and Ginkgo biloba L. extract (GBE) were administered to mice separately and in combination. Apoptotic markers and related signaling pathways were measured. Tumor weights were compared and survival analysis was used to investigate latency periods in the three treatment groups compared to a PBS control group. Results The mice administered GBE and CTX had significantly lower tumor weights compared with those administered PBS (p=0.01), however, the mice administered CTX did not have significantly lower tumor weight compared with those administered PBS (p=0.19). The expression of NF-κB, IκB-ɑ, and the phosphorylation of NF-κB and IκB-ɑ were all significantly decreased in the tumor from GBE+CTX (p<0.05). Moreover, the ratio of Bax/Bcl-2 was significantly higher for the tumor of mice administered GBE+CTX than that of mice administered GBE, CTX, or PBS (p<0.05), the expression of FADD, Caspase-8, and Cyto-C was significantly increased in the tumors of mice administered GBE+CTX than those administered PBS or GBE (p<0.05). Conclusions Application of an antioxidant at an early stage of tumor transplantation could help chemotherapeutic agent inhibit the growth of tumor cells. GBE+CTX treated mice showed a longer latency period to tumor development and markers that consistently indicated greater apoptotic activity and reduced tumor promotion.
Application of Remote Online Learning in Oral Histopathology Teaching: An Acute Response to the COVID-19 Pandemic&nbsp;&nbsp;
Authors: zhong yi , Sun Wen , Zhang Wei , Liu Laikui , Xu Yan , Jiang Yue 
Published: August 2020

Abstract
Background: The aim of this study was to investigate the experience of the combination of remote learning and virtual microscopy in oral histopathology teaching, a unique experience in China in response to the Covid-19 pandemic.Methods: 192 third-year dental students were recruited to participate in the course independently on the E-learning platform and Virtual Simulation Experiment Teaching Center of Stomatology of Nanjing Medical University. A questionnaire survey explored students' satisfaction with the education. Differences of assessment results among different groups were compared using Mann-Whitney U and independent-sample T tests.Results:The mean Theory test scores of the Online group (80.93±12.15) were significantly higher than those of the Traditional group (73.65±8.46) (P < 0.01). The mean total scores of the Online group (82.94±10.76) were significantly higher than those of the Traditional group (77.25±7.55) (P < 0.01). The percentage of high total test score (test score > 85) of the Online group (54%) was also significantly higher than that of the Traditional group (15%) (P< 0.01). Furthermore, both remote learning and virtual microscopy courses were well accepted by students according to the questionnaire. Conclusions: The application of remote learning and virtual microscopy have enhanced oral histopathology teaching in China in response to the Covid-19 pandemic.
Gut microbiota in homologous Chinese soft-shell turtle (Pelodiscus sinensis) under different habitats
Authors: Wu Benli , Huang Long , Chen Jing , Zhang Ye , Wang Jun , He Jixiang 
Published: August 2020

Abstract
Background

Chinese soft-shell turtle (Pelodiscus sinensis) is an important commercial species for its high nutritive and medicinal value, but it has been a vulnerable species due to habitat loss. In this study, homologous juvenile turtles were allocated to lake, pond and paddy field to investigate the habitat effects on turtles.
Results

The growth, morphology and gut microbial communities were monitored during the 4 months cultural period. It showed higher growth rate of turtles in paddy field and pond. The appearance, visceral coefficients, gut morphology and microbial communities in turtles were distinct among different habitats, which was mainly attributed to food abundance and living space. The diversity of gut microbial community was obviously higher in turtles from paddy field than pond and lake. Significant differences on dominant phyla, genera and species were found among initial samples and subsequent samples from different habitats. Firmicutes was the most abundant phylum in turtle gut sampled from greenhouse at initial and Proteobacteria was the most abundant phylum after cultivated in different habitats. The functional predictions indicated that both habitat type and sampling time had significant effects on metabolic pathways, especially amino acid and carbohydrate metabolism.
Conclusion

The microbial abundance was different among the habitats and varied within cultural periods. Gut microbes in turtles from paddy field presented high diversity and richness. Rice-turtle coculture was a potential ecological and economic farming mode for its high production and quality of both turtles and rice, which would play important roles in wild turtle protection, food security and paddy field environment improvement.
Periodic satellite orbits of the Broucke-Hadjidemetriou-Hénon family of three-body system with unequal masses
Authors: Li Xiaoming , Liao Shijun 
Published: August 2020

Abstract
Triple systems are common and key objects in astronomy. The three-body problem has received much more attention in recent years [1–3]. All observed periodic triple stars systems [4–6] belong to the Broucke-Hadjidemetriou-Hénon’s (BHH) family [7–9]. The BHH orbits are a family of periodic orbits of the three-body system with the simplest topological free group word [10] a, while Jankovíc and Dmitrasinovíc [1] gained 58 equal-mass BHH satellite orbits which have free group words ak (k > 1), where k is the topological exponent. However, the BHH satellite orbits with equal mass is lack of realistic meaning because they do not exist in practice. Here we present 419743 new BHH orbits and 179253 new BHH satellites (k > 1) for three-body system with unequal mass. Especially, 48761 among the 179253 new BHH satellites are stable and have unequal masses. It suggests that these 48761 stable BHH satellites could be found by the observation. Besides, for the three-body system with equal mass at a fixed energy, it was demonstrated that the relationship between the angular momentum (L) and topological scaled period (T/k) of the BHH satellites is the same as that of the BHH orbits [1]. However, we found that this does not hold for the three-body system with unequal mass. Our findings have broad impact for the astrophysical scenario: they could inspire the theoretical and observational study of the triple system, the formation of triple stars [11], the gravitational waves pattern [12] and the gravitational waves observation [13] of the triple system.
Sericin Preparation from Cocoons of Oak Tasar Silkworm Antheraea proylei J. Induces Apoptosis in a Caspase-dependent Manner in A549 and HeLa Cells and Caspase-independent Manner in PC3 Cells
Authors: Devi Potsangbam Jolly , Singh Asem Robinson , Singh Lisam Shanjukumar , Singh Laishram Rupachandra , Devi Sanjenbam Kunjeshwori 
Published: August 2020

Abstract
BackgroundDespite much progress in understanding the biology of cancer disease, advancement in technology for early diagnosis, the expanding array of anticancer drugs, and treatment modalities, the global cancer burden is still significant and increasing. It is estimated that the new cases of cancer in the year 2040 will be 29.4 million per year globally. Sericin, an adhesive protein of silk cocoon, is a potential protein in various biomedical applications including cancer therapeutics. The present study evaluates the anticancer property of sericin prepared from cocoons of Antheraea proylei J. (A. proylei ) against human lung cancer (A549), cervical cancer (HeLa), and prostate cancer (PC3) cell lines. This is the first report of the anti-cancer activity of the non-mulberry silkworm A. proylei. MethodsSericin preparation (SP) was prepared from cocoons of A. proylei J. by the process of the degumming method. The amino acid composition of the SP was determined by HPLC. Cytotoxicity activity was assessed by MTT assay and genotoxicity activity was assessed by comet assay. Cleavage of caspase and PARP proteins and phosphorylation of MAPK pathway members were analyzed by Western blotting. Cell cycle analysis was done by FACS flow cytometry.Results SP causes cytotoxicity to A549, HeLa, and PC3 cell lines with the IC50 values ranging from 3.4-3.9 µg/µl. SP induces apoptosis in a dose-dependent manner through caspase-3 and p38/SAPK/ERK pathways in A549 and HeLa cells whereas in PC3 cells SP induces apoptosis independent of caspase but through p38 pathway. Moreover, in the case of A549 and HeLa cells, SP induces cell cycle arrest at the S phase whereas at the G0 phase in the case of PC3 cells in a dose-dependent manner.ConclusionThe difference in the molecular mechanisms of apoptosis induced by SP in A549 and HeLa cell lines, and in PC3 cell lines may be due to the difference in the genotypes of the cancer cell lines where A549 and HeLa cells are being non-malignant and p53 positive whereas PC3 cell is being malignant and p53 negative. The overall results of the present study envisage the possibility of using SP as an anti-tumorogenic agent.
Clinical Correlates of Early IgG Response in Patients with Mild COVID-19
Authors: Kessler Asa , Kenig Ariel , Azmanov Henny , Gelman Ram , Hurvitz Noa , Perets Itay , Oiknine-Djian Esther , Bdolah-Abram Tali , Zelig Orly , Rotstein Zeev , Ilan Yaron , Wolf Dana G. 
Published: August 2020

Abstract
Background: We determined the temporal pattern of early SARS-CoV-2 IgG response in patients with mild COVID-19, and sought to identify predictive clinical and laboratory features. Methods: Serum samples were prospectively obtained from 111 convalescent COVID-19 patients, staying in dedicated Isolation–hotels, and tested for the presence of SARS-CoV-2 IgG by anti-S1 protein ELISA.Results: SARS-CoV-2 IgG was detected in 78 (70.3%) patients tested within the first month from diagnosis. While highly variable between patients, the rate of antibody detection generally increased with time, from 47.1% to 93.8% at the first and fourth weeks from diagnosis, respectively, with the largest shift observed between the second and third week. Notably, the presence of more profound symptoms at presentation, namely, fever and chills, positively and independently correlated with early antibody response. IgG-positive patients had higher ferritin levels (p=0.039). Older age (p<0.001) and increased CRP levels (p=0.001) were associated with higher SARS-CoV-2 IgG levels. Conclusions: The identified temporal pattern along with the correlation between inflammation-related clinical and laboratory parameters and early IgG response in patients with mild COVID-19, could provide a basis for better prediction and understating of the immune response to SARS-CoV-2, and inform therapeutic donor-plasma selection.
Human Epididymis Protein 4 Expression in the Renal Cortex Tissue is an Independent Prognostic Factor in Patients with Renal Cell Carcinoma
Authors: Noguchi Go , Jikuya Ryosuke , Miyagi Yohei , Nakamura Yoshiyasu , Ohtake Shinji , Shibata Yosuke , Suzuki Takahisa , Osaka Kimito , Umemoto Susumu , Nagashima Yoji , Yokose Tomoyuki , Kishida Takeshi , Yao Masahiro , Nakaigawa Noboru 
Published: August 2020

Abstract
Background: Human epididymis protein 4 (HE4) has been indicated as a novel tumor marker for ovarian cancer diagnosis/monitoring and as a mediator of renal fibrosis. In this study, we investigated the potential role of HE4 in the pathogenesis of patients with renal cell carcinoma (RCC).Methods: We retrospectively enrolled 249 patients with RCC who underwent nephrectomies at a single center between 1997 and 2010. HE4 expression was evaluated by immunohistochemistry and RNA in situ hybridization staining in both tumor and renal cortex tissue microarrays. The expression of HE4 was evaluated in line with the clinicopathologic features of each patient.Results: HE4-positive staining was confirmed in 37 (15.0%) tumor tissue microarrays and in 88 (38.3%) cortex tissue microarrays. High expression of HE4 in the tumor was not associated with overall survival (OS), cancer-specific survival (CSS), or recurrence free survival (RFS). However, high expression of HE4 in the renal cortex was strongly associated with an increased risk of OS, CSS, and RFS (HR 3.608 (95% confidence interval (CI) 2.127-6.118); p<0.001, HR 5.759 (95% CI 2.801-11.841); p<0.001 and HR 3.309 (95% CI 1.751-6.252); p<0.001, respectively). Multivariate analysis revealed that the Eastern Cooperative Oncology Scale of Performance Status (ECOG-PS), stage, pathological subtype, and high expression of HE4 in the renal cortex were independent risk factors for poor OS and that ECOG-PS, tumor size, stage, and high expression of HE4 in the renal cortex were independent risk factors for poor CSS.Conclusions: High expression of HE4 in the renal cortex is an independent risk factor of OS and CSS in RCC. Further investigations are needed to elucidate the underlying mechanisms of HE4 in the renal cortex in the progression of renal cell carcinoma.
Expression and Prognosis Analyses of Insulin-Like Growth Factor 2 mRNA Binding Protein Family in Human Pancreatic Cancer
Authors: Cui Xiao-Han , Hu Shu-Yi , Zhu Chun-Fu , Qin Xihu 
Published: August 2020

Abstract
Background: Despite advances in early diagnosis and treatment, cancer remains the leading cause of mortality worldwide. Insulin-like Growth Factor 2 mRNA Binding Protein (IGF2BP) family had been reported to be involved in a variety of human malignant tumors. However, little was known about their expression and prognostic value in human pancreatic cancer. So, we performed a detailed cancer versus normal differential analysis. Methods: The Cancer Genome Atlas (TCGA) and Gene Expression Profiles Interactive Analysis (GEPIA) databases were performed to analyze the mRNA expression levels of the IGF2BP family in various cancers, including pancreatic cancer. Then, the LinkedOmics and GEPIA databases were used to assess the relation between the expression levels of IGF2BPs and overall survival (OS). Then, univariate and multivariate COX regression analysis were established and subgroup of Grade&Stage were analyzed. The signaling pathway associated with IGF2BP2 and IGF2BP3 were then investigated via gene set enrichment analysis. Results: IGF2BP2 and IGF2BP3 were found to be associated with each subset of OS and Grade&Stage. Further clinical correlation analysis of IGF2BP2 and IGF2BP3 confirmed that IGF2BP2 and IGF2BP3 were fundamental factors in promoting pancreatic cancer progression.Conclusion: IGF2BP2 and IGF2BP3 were key factors in promoting the progression of pancreatic cancer and was closely related to overall survival.
Robot-assisted treatment of epigastric hernias with a suprapubic approach
Authors: Pini Ramon , Giuseppe Matteo Di , Toti Johannes Maria Alberto , Mongelli Francesco , Marcantonio Maria , Spampatti Sebastiano , Regina Davide La 
Published: August 2020

Abstract
BackgroundRobotic ventral hernia repair has shown itself to be feasible and safe in abdominal wall surgery. Presently, the ports are commonly placed laterally to meet the distance from the fascial defect. The aim of our study is to reort our experience of epigastric hernias treatment with the trocars’ insertion in the suprapubic region.MethodsA retrospective search on a prospectively collected dataset was performed to identify patients treated for primary or incisional hernias of the epigastric region with a robotic-assisted approach. In all cases, three 8-mm trocars were inserted in the suprapubic area and the preperitoneal or the posterior rectus sheath spaces dissected to ensure a proper mesh overlap. After hernia reduction, the fascia was closed with a running suture, the mesh placed and the peritoneum sutured.ResultsTwelve patients were selected. Median age was 58.5 years (interquartile range (IQR) 47.8–67.3) and four patients were male (33.3%). All patients were referred to surgery because of pain. The median measure of the hernia defect was 30 mm (IQR 13.75-31), median larger mesh diameter was 13.5 cm (IQR 9.5–15.0) and median operative time was 136.5 minutes (IQR 120-186.5). No intraoperative complication or conversion to open surgery occurred. Postoperatively, two patients presented a seroma and the median length of hospital stay was 2.0 days (IQR 1.75-3).ConclusionsIn the robot-assisted treatment of hernias of the epigastric region, a suprapubic port placement can be considered instead of a lateral one. Further studies are needed to assess the benefits and limitations of such technique.Trial registrationRetrospectively registered (Comitato Etico Cantonale Ticino n. 2019-01132 CE3495)
Gene Expression and Regulatory Webwork of POLR2K in Bladder Carcinogenesis by Integrated Bioinformatics Approaches
Authors: Yang Liliang , Wang Kaizhen , Guo Wenjing , Chen Xian , Guo Qinglong , Wei Libin , Zhou Yuxin 
Published: August 2020

Abstract
Background：RNA polymerase II subunit K (POLR2K) belongs to one of the multiple subunits of RNA polymerase II (Pol II), whose biological function is to synthesize mRNA. Aberrant POLR2K expression is related to carcinogenesis. However, POLR2K’s underlying role in bladder cancer has not been explored. In the current study, we intend to analyze the function of POLR2K and its regulatory network within bladder cancer.Methods: Public sequencing data was obtained from GEO and TCGA to investigate POLR2K expression and regulatory network within bladder cancer (BLCA) by using GEPIA and Oncomine as well as cBioPortal online tool. LinkedOmics was employed to identify genes displaying significantly differential expression patterns and to perform GO and KEGG analyses. After differential genes was assigned and ranked, GSEA analyses was performed to obtain target networks for transcription factors, miRNAs, and kinases that could regulate POLR2K–associated gene network. Subsequent functional webwork analyses were used to identify cancer-relevant pathways Moreover, POLR2K gene is verified, by ChIP-seq in MCF-7 cell line , with transcription factor binding evidence in the ENCODE Transcription Factor Binding Site Profiles dataset.Conclusions: The current study implies that POLR2K gene is overexpressed and often amplified in BLCA, providing the first evidence that POLR2K deregulation, in particular increased transcription, may promote BLCA. These findings uncover a unique expression patterns of POLR2K and its potential regulatory networks in BLCA, contributing greatly to study of the role of POLR2K in cancer development.