bluuebunny/crossref_metadata_2025_split huggingface dataset:
Continuation of Over-the-Counter Biotin Supplements in the Inpatient Setting: An Unexpected Source of Laboratory Error
Authors: Merrill Anna E , Malvik Natalie M , Ford Diana C , Krasowski Matthew D 
Published: September 2020

Abstract

Background
Over the past decade, use of high-dose biotin has increased significantly and can lead to erroneous results on some clinical immunoassays. In collaboration with pharmacists at our institution, we discovered that high biotin doses were being administered to inpatients as a continuation of patient-reported home biotin use.

Methods
This retrospective study evaluated high-dose biotin administration in 226 inpatient encounters from 2009 to 2019 and its potential impact on concurrent immunoassay testing.

Results
In 96% of cases, biotin was administered in the inpatient setting as a continuation of patient-reported home use. In total, 322 immunoassays capable of biotin interference were performed across 100 inpatient encounters with high-dose biotin administration. Troponin T and TSH were the most commonly performed immunoassays in this cohort.

Discussion
Even though less than 5% of all high-dose biotin orders at our institution are placed for inpatients, hospitalized patients are still at risk for mismanagement due to erroneous immunoassay results. Immunoassay testing susceptible to biotin interference was performed in approximately 45% of inpatient encounters with biotin administration. Laboratories utilizing biotin-susceptible, sensitive cardiac troponin assays should be particularly cautious. Pharmacokinetic data for biotin clearance is especially lacking for certain populations likely to be hospitalized, such as those with renal failure. Given that medical conditions requiring high-dose biotin therapy are extremely rare, we recommend restricting biotin dosing during inpatient encounters for all other patients.
Lethal and Sublethal Effects of Two Commercial Insecticides on Egg Parasitoids (Hymenoptera: Scelionidae) of Green Stink Bugs (Hem: Pentatomidae)
Authors: Ranjbar Fateme , Reitz Stuart , Jalali M Amin , Ziaaddini Mahdi , Izadi Hamzeh 
Published: September 2020

Abstract
Pistacia vera L. is one of the most important horticultural crops in Iran. The stink bugs Acrosternum arabicum and Brachynema germari are two of the key pests that cause significant direct and indirect damage on Pistacia vera. Egg parasitoids have been considered as potential biological control agents of pistachio green stink bugs. Among them, Trissolcus semistriatus and Psix saccharicola are the most abundant and efficient parasitoid for A. arabicum in pistachio orchards. In this study, we assessed lethal and sublethal effects of two commonly used insecticide products (fenitrothion and a binary mixture of lambda-cyhalothrin + thiamethoxam) on these two parasitoid wasps under laboratory conditions. The median lethal concentration (LC50) values for fenitrothion and thiamethoxam + lambda-cyhalothrin in P. saccharicola and T. semistriatus were estimated as 1.03, 0.48 and 0.87, 0.98 mg a.i./liter, respectively. In terms of sublethal effects, insecticide treatments altered the type of functional response from type III to type II in T. semistriatus. However, P. saccharicola exhibited a type II functional to density of A. arabicum for all treatments, although attack rates were lower for insecticide-exposed wasps while handling times increased. Our results show that sublethal effects of insecticides further reduce the efficacy of biological control agents. Effective integrated pest management programs should avoid antagonistic interactions between chemical and biological control methods. The results of this study provide useful information to develop comprehensive integrated pest management programs for stink bugs in pistachio orchards.
Monitoring House Fly (Diptera: Muscidae) Activity on Animal Facilities
Authors: Gerry Alec C 
Published: September 2020

AbstractMonitoring house fly (Diptera: Muscidae) activity on animal facilities is a necessary component of an integrated pest management (IPM) program to reduce the negative impacts of these flies. This article describes monitoring methods appropriate for use on animal facilities with discussion of monitoring device use and placement. Action thresholds are presented where these have been suggested by researchers. Sampling precision is an important aspect of a monitoring program, and the number of monitoring devices needed to detect a doubling of fly activity is presented for monitoring methods where this information is available. It should be noted that both action thresholds and numbers of monitoring devices will be different for every animal facility. Suggested action thresholds and numbers of monitoring devices are presented only to provide guidance when initiating a fly monitoring program. Facility managers can adjust these values based upon the fly activity data recorded at their facility. Spot cards are generally recommended as an easy-to-use method for monitoring fly activity for most animal facilities. Fly ribbons or similar sticky devices are recommended where several pest fly species may be abundant and identifying the activity of each species is important, but a sampling period of &lt;7 d may be needed in dusty conditions or when fly density is high. Fly ribbons are not recommended for outdoor use. Insecticide-baited traps may be used in outdoor locations where environmental conditions limit the use of spot cards, fly ribbons, and sticky traps.
Morphological and Chromatic Variation in Four Populations of Triatoma mexicana (Hemiptera: Reduviidae)
Authors: Rivas Nancy , Sánchez-Cordero Victor , Camacho Alejandro D , Alejandre-Aguilar Ricardo 
Published: September 2020

Abstract
Triatoma mexicana is an endemic species of Mexico and is distributed in the states of Hidalgo, Queretaro, Guanajuato, and San Luis Potosi, being naturally infected with Trypanosoma cruzi, which increases its importance in the region. The species description was made in 1848, but there are only a few studies on its morphology, biology, and behavior. The present manuscript shows the presence of morphological and chromatic variations among populations of T. mexicana from the states of Hidalgo (Valle del Mezquital and Meztitlan), Guanajuato and Queretaro. The study employed 136 specimens collected in four locations. Scanning electron microscopy was used to study the morphological characteristics of the head, pronotum, and scutellum; also, we measured the width of the abdomen and the total length in the specimens of each population. The morphometric analysis considered 19 variables in the previous structures. Significant differences were found in the dimensions of the head and pronotum, but not in the scutellum; there is clear discrimination among the four proposed populations. The chromatic patterns observed in the connexivum go from yellow to brown and show some significant differences related to geographical origin. The set of evaluated characters showed a higher degree of difference in the population of Guanajuato, clearly separating from the rest of the populations, indicating the possibility of a divergence process. The characteristics observed in the remaining populations could be adaptive responses to their habitat.
Average cometary ion flow pattern in the vicinity of comet 67P from moment data
Authors: Nilsson Hans , Williamson Hayley , Bergman Sofia , Stenberg Wieser Gabriella , Wieser Martin , Behar Etienne , Eriksson Anders I , Johansson Fredrik L , Richter Ingo , Goetz Charlotte 
Published: September 2020

ABSTRACT
Average flow patterns of ions around comet 67P detected by the RPC-ICA instrument onboard Rosetta are presented both as a time series and as a spatial distribution of the average flow in the plane perpendicular to the comet – Sun direction (Y–Z plane in the coordinate systems used). Cometary ions in the energy range up to 60 eV flow radially away from the nucleus in the Y–Z plane, irrespective of the direction of the magnetic field, throughout the mission. These ions may however be strongly affected by the spacecraft potential, the uncertainty due to this is briefly discussed. Inside the solar wind ion cavity and in the periods just before and after, the cometary pick up ions moving antisunward are deflected against the inferred solar wind electric field direction. This is opposite to what is observed for lower levels of mass-loading. These pick up ions are behaving in a similar way to the solar wind ions and are deflected due to mass-loading. A spatial asymmetry can be seen in the observations of deflected pick up ions, with motion against the electric field primarily within a radius of 200 km of the nucleus and also in the negative electric field hemisphere. Cometary ions observed by RPC-ICA typically move in the antisunward direction throughout the mission. These are average patterns, full-resolution data show very much variability.
Daytime optical turbulence profiling with a profiler of the differential solar limb
Authors: Song Tengfei , Cai Zhanchuan , Liu Yu , Zhao Mingyu , Fang Yuliang , Zhang Xuefei , Wang Jingxing , Li Xiaobo , Song Qiwu , Du Zhimao 
Published: September 2020

ABSTRACT
Atmospheric turbulence reduces the image quality and resolution of ground-based optical telescopes. Future large solar telescopes (e.g. the CGST, China Giant Solar Telescope) should be equipped with adaptive optics (AO) systems. The design of AO systems is associated with atmospheric optical turbulence parameters, especially the profile of the refractive index structure $C_{n}^{2}(h)$. With the solar differential image motion monitor (S-DIMM) and the profiler of the moon limb (PML), a simplified version of a PML, termed a profiler of the differential solar limb (PDSL), was built in order to determine the daytime $C_{n}^{2}(h)$ and other atmospheric turbulence parameters. A PDSL with differential solar limb fluctuations was used to determine the turbulence profiling, and the extended solar limb extends the range of separation angles for a higher resolution of the height profile. The PDSL structure and its performance are described. In addition, numerical simulations were conducted to verify the effectiveness of the method. As revealed from the simulation results, the layered integral coefficient matrix is capable of solving the discretization error and enhancing the inversion accuracy of the turbulence contour. The first test results at Mt Wumingshan (a candidate site for the CGST) are presented.
Are the Milky Way and Andromeda unusual? A comparison with Milky Way and Andromeda analogues
Authors: Boardman N , Zasowski G , Newman J A , Andrews B , Fielder C , Bershady M , Brinkmann J , Drory N , Krishnarao D , Lane R R , Mackereth T , Masters K , Stringfellow G S 
Published: September 2020

ABSTRACT
Our Milky Way provides a unique test case for galaxy evolution models because of our privileged position within the Milky Way’s disc. This position also complicates comparisons between the Milky Way and external galaxies, due to our inability to observe the Milky Way from an external point of view. Milky Way analogue galaxies offer us a chance to bridge this divide by providing the external perspective that we otherwise lack. However, overprecise definitions of ‘analogue’ yield little-to-no galaxies, so it is vital to understand which selection criteria produce the most meaningful analogue samples. To address this, we compare the properties of complementary samples of Milky Way analogues selected using different criteria. We find the Milky Way to be within 1σ of its analogues in terms of star formation rate and bulge-to-total ratio in most cases, but we find larger offsets between the Milky Way and its analogues in terms of disc scale length; this suggests that scale length must be included in analogue selections in addition to other criteria if the most accurate analogues are to be selected. We also apply our methodology to the neighbouring Andromeda galaxy. We find analogues selected on the basis of strong morphological features to display much higher star formation rates than Andromeda, and we also find analogues selected on Andromeda’s star formation rate to overpredict Andromeda’s bulge extent. This suggests both structure and star formation rate should be considered when selecting the most stringent Andromeda analogues.
Tracing circumnuclear dense gas in H2O maser galaxies
Authors: Farhan A , Ercan E N , Tombesi F 
Published: September 2020

ABSTRACT
A sample of 30 H$_{2}$O extra-galactic maser galaxies with their published HCN(J = 1 − 0) and HCO + (J = 1 − 0) observations has been compiled to investigate the dense gas correlation with H2O maser emission. Our sample number exceeds the size of the previous HCN samples studied so far by a factor of 3, and it is the first study on the possible relation with the dense gas tracer HCO + . We find a strong correlation between normalized H2O maser emission luminosity (LH2O/LCO) and normalized HCO + line luminosity (LHCO + /LCO). Moreover, a weak correlation has been found between LH2O/LCO and normalized HCN line luminosity (LHCN/LCO). The sample is also studied after excluding Luminous and ultraluminous infrared galaxy (U)LIRG sources, and the mentioned correlations are noticeably stronger. We show that ‘dense gas’ fractions as obtained from HCN and HCO + molecules tightly correlate with maser emission, especially for galaxies with normal IR luminosity (LIR &lt; 1011 L⊙) and we show that HCO + is a better ‘dense gas’ tracer than HCN. Further systematic studies of these dense gas tracers with higher transition level lines are vital to probe megamaser physical conditions and to accurately determining how maser emission interrelates with the dense gas.
Readthrough of stop codons under limiting ABCE1 concentration involves frameshifting and inhibits nonsense-mediated mRNA decay
Authors: Annibaldis Giuditta , Domanski Michal , Dreos René , Contu Lara , Carl Sarah , Kläy Nina , Mühlemann Oliver 
Published: September 2020

AbstractTo gain insight into the mechanistic link between translation termination and nonsense-mediated mRNA decay (NMD), we depleted the ribosome recycling factor ABCE1 in human cells, resulting in an upregulation of NMD-sensitive mRNAs. Suppression of NMD on these mRNAs occurs prior to their SMG6-mediated endonucleolytic cleavage. ABCE1 depletion caused ribosome stalling at termination codons (TCs) and increased ribosome occupancy in 3′ UTRs, implying enhanced TC readthrough. ABCE1 knockdown indeed increased the rate of readthrough and continuation of translation in different reading frames, providing a possible explanation for the observed NMD inhibition, since enhanced readthrough displaces NMD activating proteins from the 3′ UTR. Our results indicate that stalling at TCs triggers ribosome collisions and activates ribosome quality control. Collectively, we show that improper translation termination can lead to readthrough of the TC, presumably due to ribosome collisions pushing the stalled ribosomes into the 3′ UTR, where it can resume translation in-frame as well as out-of-frame.
NuA3 HAT antagonizes the Rpd3S and Rpd3L HDACs to optimize mRNA and lncRNA expression dynamics
Authors: Kim Ji Hyun , Yoon Chae Young , Jun Yukyung , Lee Bo Bae , Lee Ji Eun , Ha So Dam , Woo Hyeonju , Choi Ahyoung , Lee Sanghyuk , Jeong Woojin , Kim Ji Hyung , Kim TaeSoo 
Published: September 2020

AbstractIn yeast, NuA3 histone acetyltransferase (NuA3 HAT) promotes acetylation of histone H3 lysine 14 (H3K14) and transcription of a subset of genes through interaction between the Yng1 plant homeodomain (PHD) finger and H3K4me3. Although NuA3 HAT has multiple chromatin binding modules with distinct specificities, their interdependence and combinatorial actions in chromatin binding and transcription remain unknown. Modified peptide pulldown assays reveal that the Yng1 N-terminal region is important for the integrity of NuA3 HAT by mediating the interaction between core subunits and two methyl-binding proteins, Yng1 and Pdp3. We further uncover that NuA3 HAT contributes to the regulation of mRNA and lncRNA expression dynamics by antagonizing the histone deacetylases (HDACs) Rpd3S and Rpd3L. The Yng1 N-terminal region, the Nto1 PHD finger and Pdp3 are important for optimal induction of mRNA and lncRNA transcription repressed by the Set2-Rpd3S HDAC pathway, whereas the Yng1 PHD finger–H3K4me3 interaction affects transcriptional repression memory regulated by Rpd3L HDAC. These findings suggest that NuA3 HAT uses distinct chromatin readers to compete with two Rpd3-containing HDACs to optimize mRNA and lncRNA expression dynamics.
Electronic Cigarette Liquid Constituents Induce Nasal and Tracheal Sensory Irritation in Mice in Regionally Dependent Fashion
Authors: Ni Fenge , Ogura Tatsuya , Lin Weihong 
Published: September 2020

Abstract

Introduction
Electronic cigarettes (e-cigs) are currently used by millions of adults and adolescents worldwide. Major respiratory symptoms, such as coughing reported by e-cig users, including patients with e-cig, or vaping, product use-associated lung injury (EVALI), indicate e-cig constituent-induced sensory irritation. However, e-cig constituent-induced nociceptive activity in nasal and tracheal respiratory epithelia (RE) and neuronal activation in the trigeminal ganglia and brainstem nuclei, which receive airway chemosensory inputs have not been examined and compared. Comparisons of physiological responses between freebase nicotine and nicotine salts are also missing.

Aims and Methods
Event-related potential (ERP) was recorded electrophysiologically to assess mouse nasal and tracheal RE chemosensory responses to various flavorings, nicotine, including freebase and nicotine salts, e-liquid mixtures, and tussigenic stimuli. Also, mice were subjected to inhalation exposure to aerosol of a vanilla-flavored e-liquid or air (control), and the activated-trigeminal nociceptive neurons and brainstem neurons were examined using immunohistochemistry.

Results
Individual constituents and mixtures of e-liquids, capsaicin, and citric and acetic acids evoked significantly larger ERP in the nose than in the trachea with the exception of menthol. ERP responses to freebase nicotine were significantly larger than protonated nicotine. Four nicotine salts (benzoate, lactate, levulinate, and salicylate) induced similar responses. Compared with air-exposed mice, e-liquid aerosol-exposed mice showed a significant increase in numbers of activated trigeminal nociceptive neurons and brainstem neurons in the spinal trigeminal nucleus, paratrigeminal nucleus, and nucleus tractus solitarius.

Conclusions
E-liquid constituents region-dependently stimulate airway nociceptive chemosensory systems, and freebase nicotine is more potent than protonated nicotine.

Implications
Neural abnormalities have been implicated in the development of nasal and respiratory illnesses. The higher sensitivity of the nasal nociceptive chemosensory system to nicotine and flavorings may indicate a health risk for e-liquid aerosol-induced upper airway illnesses via neurogenic alteration and warrants further investigation.
Unlocking the Legal Deadlock over Dreadlocks in Kenyan Schools: Constitutional Law, Rastafarians, and Religious Freedom
Authors: Sang YK Brian 
Published: September 2020

Abstract
Legal recognition of Rastafari as a religion is a crucial step in enabling its adherents to enjoy the full scope of their religious freedom. This article considers and critiques the legal implications of the High Court of Kenya’s decision in JWM (alias P) v Board of Management O High School. In JWM, the headteacher of a secondary school decided that a Rastafarian girl who wore dreadlocks for religious reasons should be excluded from the school and only be readmitted once she had cut them as her hairstyle breached school rules. The High Court concluded that this was a violation of the student’s right to education and religious freedom. Though welcoming the JWM verdict as legally correct, this article reflects critically on how the Court arrived at its conclusion. It reviews the High Court’s reasoning in JWM and offers a constructive analysis of the likely effect of JWM on Kenyan education institutions and their uniform policies. The article advocates the need for principled, context-sensitive and methodical approaches to adjudicating freedom of religion claims so as to protect the rights of religious believers and secularists alike.
COVID-19 and Public Policy Imperatives: A Trainee Call to Action
Authors: Blum James R. , Feuerbach Alec M. , Fox Jacob A. , Rook Jordan M. 
Published: September 2020

The COVID-19 pandemic has exacerbated the flaws in the U.S. employer-based health insurance system, magnified racial disparities in health and health care, and overwhelmed the country’s underfunded public health infrastructure. These are the same systematic failures that have always harmed and killed the nation’s most vulnerable. While everyone wishes for an end to this national tragedy, the authors believe a new normal must be defined for the postpandemic period.
In the postpandemic period, policies that were once labeled radical and impossible will be urgent and necessary. Examples of such policies include providing universal health care, dismantling the structures that propagate racism and injustice, and reinvesting in public health. Previous research by the authors has shown that their medical student colleagues recognize that it is their responsibility to address policies that harm patients and to support reforms at the scale the authors propose. This commitment to a better future is reflected in the widespread mobilization of medical students seen across the United States. Recognizing that the old normal is unsustainable, the authors call on those who previously benefited from the status quo to instead seek a new postpandemic normal that works for all.
Age-Dependent and Seasonal Changes in Menstrual Cycle Length and Body Temperature Based on Big Data
Authors: Tatsumi Takayuki , Sampei Makiko , Saito Kazuki , Honda Yuka , Okazaki Yuka , Arata Naoko , Narumi Kanako , Morisaki Naho , Ishikawa Tomonori , Narumi Satoshi 
Published: September 2020

OBJECTIVE:To evaluate the effects of age and season on menstrual cycle length and basal body temperature (BBT). We also examined the effects of climate on cycle length and BBT, taking into account Japanese geographic and social characteristics.METHODS:In this retrospective cohort study, we analyzed data from 6 million menstrual cycles entered into a smartphone application from 310,000 females from 2016 to 2017. Only those who entered more than 10 cycles in 2 years were included. Generalized estimation equations were used to adjust for confounding factors and for within-person correlations of multiple records. Multiple regression analysis was conducted, with age, external average temperature, precipitation amount, and sunshine hours as confounding factors.RESULTS:The mean menstrual cycle length increased from age 15–23 years, subsequently decreased up to age 45 years, and then increased again. Average follicular phase body temperature showed no significant age-dependent changes, but luteal phase body temperature gradually increased up to 29 years and then stabilized and started to decrease after age 42 years. A significant association between external temperature and body temperature (follicular and luteal phase) was observed, though menstrual cycle length did not show such an association.CONCLUSION:These results, derived from data self-entered into a smartphone application, revealed underrecognized age-dependent and seasonal changes in menstrual cycle length and BBT, which will contribute to a better understanding of female reproductive health in the modern world.