bluuebunny/crossref_metadata_2025_split huggingface dataset:
Intra‐accumbal dopaminergic system modulates the restraint stress‐induced antinociceptive behaviours in persistent inflammatory pain
Authors: Faramarzi Golnaz , Charmchi Elham , Salehi Sakineh , Zendehdel Morteza , Haghparast Abbas 
Published: December 2020

AbstractBackgroundStress activates several neural pathways that inhibit pain sensation. Nucleus accumbens (NAc), as an important component of the mesolimbic dopaminergic system, has a major role in pain modulation and is differentially affected by stress. Based on the nature of stressors, the direction of this effect is controversial. We previously showed that forced swim stress‐induced analgesia through activation of NAc dopamine receptors. In this study, we aimed to examine the role of dopamine receptors within the NAc in restraint stress (RS)‐induced analgesia.MethodsMale Wistar rats weighing 230–250 g were unilaterally implanted with a cannula into the NAc. D1‐like dopamine receptor antagonist, SCH‐23390 (0.25, 1 and 4 µg/0.5 µL saline), or D2‐like dopamine receptor antagonist, Sulpiride (0.25, 1 and 4µg/0.5µl DMSO), were microinjected into NAc in two separate super groups 5 min prior to exposure to RS. Their control groups just received intra‐accumbal saline or DMSO (0.5 µl) respectively. The formalin test was performed after animals were subjected to RS using Plexiglas tubes.ResultsThe results demonstrated that RS produces analgesia in both phases of the formalin test. Intra‐NAc injection of SCH‐23390 equally reduced RS‐induced analgesia in both early and late phases of the formalin test, while Sulpiride reduced RS‐induced analgesia just at the late phase.ConclusionsThese findings suggest that the dopaminergic system might act as a potential endogenous pain control system in stress conditions. However, the lack of evaluation of the role of the dopaminergic system in RS‐induced antinociception in acute pain conditions is considered as a limitation for this study. In addition, a comprehensive evaluation of this endogenous pain control system in animal and clinical studies will guide future efforts for developing more effective medication.SignificanceRestraint stress (RS) induces the antinociceptive behaviors in both phases of formalin test. Blockade of intra‐accumbal dopamine receptors impresses the antinociception induced by RS. Blockade of D1‐like dopamine receptor equally reduced RS‐induced analgesia in both early and late phases of the formalin test. Blockade of D2‐like dopamine receptor reduced RS‐induced analgesia just at the late phase.
Determination of sulfonamide antibiotics in fish and shrimp samples based on magnetic carbon nanotube dummy molecularly imprinted polymer extraction followed by UPLC‐MS/MS
Authors: Gao Lei , Qin Dongli , Chen Zhongxiang , Wu Song , Tang Shizhan , Wang Peng 
Published: December 2020

AbstractIn this study, a novel purification method using magnetic solid‐phase extraction (MSPE) based on magnetic carbon nanotube dummy molecularly imprinted polymer (MCNTMIP) nanocomposite was investigated for separation and enrichment of sulfonamide antibiotics (SAs) in fish and shrimp samples. The MCNTMIP nanocomposite was successfully synthesized by applying carbon nanotubes as supporting template, methacrylic acid as functional monomer, sulfabenzamide as the dummy template for SAs, and ethylene glycol dimethacrylate as crosslinking agent, then was characterized by Fourier‐transform infrared spectrometry and vibrating sample magnetometry. The adsorption performance of MCNTMIP was evaluated by binding experiments, including static adsorption, kinetic adsorption, and selectivity recognition study. The results confirmed that an imprinted polymer layer was successfully constructed on the surface of the MCNTMIP and this sorbent has advantages of simple magnetic separation, specific molecular recognition, and high adsorption capacity. Combined with ultra‐high performance liquid chromatography tandem mass spectrometry (UPLC‐MS/MS), we developed a rapid, sensitive, efficient MSPE method for detecting SAs analytes. Under the optimal conditions, the limits of detection were low to 0.1 μg/kg, and the recoveries of SAs analytes were ranged between 90.2 and 99.9%. In addition, the precision values were ranged between 0.5 and 9.1%. This method was successfully applied to analyze SAs in fish and shrimp samples with satisfactory recoveries.
Regulatory influences of methyl jasmonate and calcium chloride on chilling injury of banana fruit during cold storage and ripening
Authors: Elbagoury Mostafa M. , Turoop Losenge , Runo Steven , Sila Daniel N. 
Published: December 2020

AbstractFruit quality is preserved through cold storage, but climacteric fruits are prone to chilling injury (CI) which limits their shelf life and marketability. Two postharvest treatments, 1 mM methyl jasmonate (MeJA) and 4% (wt/vol) calcium chloride (Ca2+), were separately used to investigate their influences on chilling injury (CI) incidence and fruit quality in unpacked banana cultivar “Grand Nain” during cold storage and subsequent ripening. Banana fruits were dipped for 2 min in aqueous emulsions containing 1% Tween‐80—used here as a surfactant with untreated fruits being used as control. Fruits were stored at 10 ± 2 or optimal 14 ± 2°C temperature and relative humidity 85%–90% for a 20‐day cold storage period and then removed from cold storage at 5, 10, 15, and 20 days followed by ripening at 22 ± 2°C. Treatments with MeJA or Ca2+ significantly reduced CI in banana fruit during cold storage and subsequent ripening temperature. Untreated controls exhibited increased CI, weight loss, and decreased hue angle, as well as firmness. In contrast, the aforementioned changes were considerably delayed after treatments with MeJA or Ca2+. Application of MeJA or Ca2+ also increased total phenolic compound contents and maintenance of total antioxidant activity throughout cold storage and during ripening periods as compared to that of the control. These findings indicate that coating bananas with 1 mM MeJA or 4% (wt/vol) Ca2+ can improve the postharvest quality and shelf life of fruits, and it can ameliorate chilling injury during cold storage and at ripening temperature.
Social cognition in the blind brain: A coordinate‐based meta‐analysis
Authors: Arioli Maria , Ricciardi Emiliano , Cattaneo Zaira 
Published: December 2020

AbstractSocial cognition skills are typically acquired on the basis of visual information (e.g., the observation of gaze, facial expressions, gestures). In light of this, a critical issue is whether and how the lack of visual experience affects neurocognitive mechanisms underlying social skills. This issue has been largely neglected in the literature on blindness, despite difficulties in social interactions may be particular salient in the life of blind individuals (especially children). Here we provide a meta‐analysis of neuroimaging studies reporting brain activations associated to the representation of self and others' in early blind individuals and in sighted controls. Our results indicate that early blindness does not critically impact on the development of the “social brain,” with social tasks performed on the basis of auditory or tactile information driving consistent activations in nodes of the action observation network, typically active during actual observation of others in sighted individuals. Interestingly though, activations along this network appeared more left‐lateralized in the blind than in sighted participants. These results may have important implications for the development of specific training programs to improve social skills in blind children and young adults.
The relationship between hippocampal subfield volumes and autobiographical memory persistence
Authors: Barry Daniel N. , Clark Ian A. , Maguire Eleanor A. 
Published: December 2020

AbstractStructural integrity of the human hippocampus is widely acknowledged to be necessary for the successful encoding and retrieval of autobiographical memories. However, evidence for an association between hippocampal volume and the ability to recall such memories in healthy individuals is mixed. Here we examined this issue further by combining two approaches. First, we focused on the anatomically distinct subregions of the hippocampus where more nuanced associations may be expressed compared to considering the whole hippocampal volume. A manual segmentation protocol of hippocampal subregions allowed us to separately calculate the volumes of the dentate gyrus/CA4, CA3/2, CA1, subiculum, pre/parasubiculum and uncus. Second, a critical feature of autobiographical memories is that they can span long time periods, and so we sought to consider how memory details persist over time by conducting a longitudinal study whereby participants had to recall the same autobiographical memories on two visits spaced 8 months apart. Overall, we found that there was no difference in the total number of internal (episodic) details produced at Visits 1 and 2. However, further probing of detail subcategories revealed that specifically the amount of subjective thoughts and emotions included during recall had declined significantly by the second visit. We also observed a strong correlation between left pre/parasubiculum volume and the amount of autobiographical memory internal details produced over time. This positive relationship was evident for particular facets of the memories, with remembered events, perceptual observations and thoughts and emotions benefitting from greater volume of the left pre/parasubiculum. These preliminary findings expand upon existing functional neuroimaging evidence by highlighting a potential link between left pre/parasubiculum volume and autobiographical memory. A larger pre/parasubiculum appears not only to protect against memory decay, but may possibly enhance memory persistence, inviting further scrutiny of the role of this brain region in remote autobiographical memory retrieval.
Watershed studies at the Hubbard Brook Experimental Forest: Building on a long legacy of research with new approaches and sources of data
Authors: Campbell John L. , Rustad Lindsey E. , Bailey Scott W. , Bernhardt Emily S. , Driscoll Charles T. , Green Mark B. , Groffman Peter M. , Lovett Gary M. , McDowell William H. , McGuire Kevin J. , Rosi Emma J. 
Published: December 2020

AbstractThe Hubbard Brook Experimental Forest (HBEF) was established in 1955 by the U.S. Department of Agriculture, Forest Service out of concerns about the effects of logging increasing flooding and erosion. To address this issue, within the HBEF hydrological and micrometeorological monitoring was initiated in small watersheds designated for harvesting experiments. The Hubbard Brook Ecosystem Study (HBES) originated in 1963, with the idea of using the small watershed approach to study element fluxes and cycling and the response of forest ecosystems to disturbances, such as forest management practices and air pollution. Early evidence of acid rain was documented at the HBEF and research by scientists at the site helped shape acid rain mitigation policies. New lines of investigation at the HBEF have built on the long legacy of watershed research resulting in a shift from comparing inputs and outputs and quantifying pools and fluxes to a more mechanistic understanding of ecosystem processes within watersheds. For example, hydropedological studies have shed light on linkages between hydrologic flow paths and soil development that provide valuable perspective for managing forests and understanding stream water quality. New high frequency in situ stream chemistry sensors are providing insights about extreme events and diurnal patterns that were indiscernible with traditional weekly sampling. Additionally, tools are being developed for visual and auditory data exploration and discovery by a broad audience. Given the unprecedented environmental change that is occurring, data from the small watersheds at the HBEF are more relevant now than ever and will continue to serve as a basis for sound environmental decision‐making.
Evidence and implications of pre‐existing humoral cross‐reactive immunity to SARS‐CoV‐2
Authors: Mveang Nzoghe Amandine , Essone Paulin N. , Leboueny Marielle , Maloupazoa Siawaya Anicet Christel , Bongho Eliode Cyrien , Mvoundza Ndjindji Ofilia , Avome Houechenou Rotimi Myrabelle , Agnandji Selidji Todagbe , Djoba Siawaya Joel Fleury 
Published: December 2020

AbstractBackgroundSevere acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) has emerged throughout the world. Building knowledge around Covid‐19 is crucial to devise facts based approaches to respond efficiently against this pandemic.AimWe aimed to investigate pre‐existing humoral cross‐reactive immunity to SARS‐CoV‐2.MethodWe have tested the reactivity against SARS‐CoV‐2 nucleocapsid (N) antigen of sera collected from healthy healthcare volunteers in 2014. We assessed immunoglobulins reactive against SARS‐CoV‐2 N‐antigen using a well‐validated serological platform; Elecsys assay.ResultsSera from 32 subjects (out of 135 [23.7%]) were reactive to SARS‐CoV‐2 N‐antigen, suggesting the presence of anti‐SARS‐CoV‐2 N‐antigen antibodies.ConclusionAlthough the clinical relevance of the observed reactivity can only be speculated and needs to be investigated, the implication of this finding for coronavirus disease 2019 seroepidemiological survey and vaccines' clinical trials is critical.
Age‐stratified reference intervals unlock the clinical potential of circulating cell‐free DNA as a biomarker of poor outcome for healthy individuals and patients with colorectal cancer
Authors: Ørntoft Mai‐Britt Worm , Jensen Sarah Østrup , Øgaard Nadia , Henriksen Tenna Vesterman , Ferm Linnea , Christensen Ib Jarle , Reinert Thomas , Larsen Ole Halfdan , Nielsen Hans Jørgen , Andersen Claus Lindbjerg 
Published: December 2020

AbstractCirculating cell‐free DNA (cfDNA) has spurred much interest as a biomarker in oncology. However, inter‐ and intra‐individual cfDNA levels vary greatly. Consequently, in order to base clinical decisions on cfDNA measurements, normal reference intervals are essential to avoid that ordinary variation is confused with clinically relevant change. The lack of reference intervals may potentially explain the ambiguous results reported in the field. Our study aimed to establish reference intervals and to evaluate the association between cfDNA and demographic and clinical variables, including colorectal cancer (CRC). Plasma samples and clinical data from 2817 subjects were collected including 1930 noncancer individuals and 887 CRC patients. cfDNA was measured using droplet digital polymerase chain reaction (PCR). The large cohort combined with robust cfDNA quantification enabled establishment of reference intervals (<67 years: 775‐4860 copies/mL; ≥67 years: 807‐6561 copies/mL). A cfDNA level above the age‐stratified 90% percentile was prognostic of reduced survival in both noncancer individuals and CRC patients, with HR values of 2.56 and 2.01, respectively. Moreover, cfDNA levels increased significantly with age, elevated BMI and chronic diseases. In CRC, the cfDNA level was increased for Stage IV, but not Stage I to Stage III cancer. In summary, the use of reference intervals revealed that high cfDNA levels were predictive of shorter survival in both noncancer individuals and CRC patients, and that CRC development did not affect the cfDNA level until metastatic dissemination. Furthermore, cfDNA levels were impacted by age and chronic diseases. Conclusively, our study presents reference intervals that will help pave the way for clinical utilization of cfDNA.
Statin use and head and neck squamous cell carcinoma outcomes
Authors: Getz Kayla R. , Bellile Emily , Zarins Katie R. , Rullman Cailey , Chinn Steven B. , Taylor Jeremy M. G. , Rozek Laura S. , Wolf Gregory T. , Mondul Alison M. 
Published: December 2020

AbstractHead and neck squamous cell carcinoma (HNSCC) is a morbid cancer with poor outcomes. Statins possess anticancer properties such as immunomodulatory and anti‐inflammatory effects. The objective of our study is to identify the association between statin use among untreated HNSCC patients and overall death, disease‐specific death and recurrence. HNSCC patients were recruited to participate in the University of Michigan Head and Neck Cancer Specialized Program of Research Excellence (SPORE) from 2003 to 2014. Statin use data were collected through medical record review. Participants were considered a statin user if they used a statin at or after diagnosis. Outcome data were collected through medical record review, Social Security Death Index or LexisNexis. Our analytic cohort included 1638 participants. Cox proportional hazard models were used to estimate the association between ever statin use and HNSCC outcomes. Statin use was seen in 36.0% of participants. We observed a statistically significant inverse association between ever using a statin and overall death (HR = 0.75, 95% CI = 0.63‐0.88) and HNSCC‐specific death (HR = 0.79, 95% CI = 0.63‐0.99) and a nonstatistically significant inverse association for recurrence (HR = 0.85, 95% CI = 0.70‐1.04). When investigating the association between statin use and HNSCC outcomes utilizing interaction terms between statin use and human papillomavirus (HPV), statistically significant interactions for HNSCC‐specific death and recurrence were identified (HNSCC‐specific death: HPV‐positive HR = 0.41, 95% CI = 0.21‐0.84; HPV‐negative HR = 1.04, 95% CI = 0.71‐1.51; p‐int=0.02; recurrence: HPV‐positive HR = 0.49, 95% CI = 0.29‐0.84; HPV‐negative HR = 1.03, 95% CI = 0.74‐1.43; p=int‐0.02). Statin use may be protective for adverse outcomes in HNSCC patients, particularly those with HPV‐positive disease. If true, these findings could have a meaningful impact on tertiary prevention for this cancer.
Natural history of oral HPV infection: Longitudinal analyses in prospective cohorts from Australia
Authors: Antonsson Annika , de Souza Marjorie , Wood Zoe C. , Carroll Angela , Van Kim , Paterson Lachlan , Pandeya Nirmala , Whiteman David C. 
Published: December 2020

AbstractOral infection with human papillomavirus (HPV) is likely to underpin the rapidly rising incidence of oropharyngeal squamous cell carcinoma; however, there are few data describing the natural history of oral HPV infection. We recruited 704 participants aged 20 to 70 years from worksites, universities and primary care practices in Brisbane, Australia. Participants completed questionnaires at baseline, 12 and 24 months and donate four saliva samples at baseline, 6, 12 and 24 months for HPV polymerase chain reaction testing and typing. We estimated the prevalence of oral HPV infection at baseline, incidence of new infections among those HPV‐negative at baseline, clearance rate and persistent infections. At baseline, 10.7% of participants had oral HPV infections from 26 different HPV types. Sexual behaviours were associated with oral HPV infection, including more partners for passionate kissing (29 or more; odds ratio [OR] 3.4, 95% confidence interval [CI] 1.5‐8.0), and giving and receiving oral sex (16 or more; OR 5.4, 95% CI 1.6‐17.7 and OR 5.6, 95% CI 1.6‐18.7, respectively). Of 343 participants, HPV‐free at baseline and with subsequent saliva samples, 87 (25%) acquired new infections over the 24 months. Sixty‐eight of 87 people included in the clearance analysis (78%) cleared their oral HPV infections. Clearance was associated with being a nonsmoker (OR 12.7, 95% CI 1.3‐122.8), and no previous diagnosis of a sexually transmitted infection (OR 6.2, 95% CI 2.0‐19.9). New oral infections with HPV in this sample were not rare. Although most infections were cleared, clearance was not universal suggesting a reservoir of infection exists that might predispose to oropharyngeal carcinogenesis.
Does mild thrombocytopenia increase peripartum hemorrhage in elective cesarean deliveries? A retrospective cohort study
Authors: Işıkalan Mehmet Murat , Özkaya Eren Berkay , Özkaya Buşra , Ferlibaş Enes , Şengül Nurullah , Acar Ali 
Published: December 2020

AbstractObjectiveTo investigate the effect of mild thrombocytopenia (platelet count: 100 000–149 000/µl) on peripartum hemorrhage in elective cesarean deliveries.MethodsThis study was conducted between January 2018 and May 2019 in a hospital, located in Konya, Turkey. Uncomplicated pregnancies undergoing elective cesarean section were included. Of 1992 eligible patients, 201 women were determined as the mild thrombocytopenia group, 48 women as the severe thrombocytopenia group, and 1743 women as the control group. The estimated blood loss volume (EBLV), the need for blood transfusion, and excessive blood loss rates were compared among groups. Logistic regression analysis was performed for potential confounding factors.ResultsThe EBLV and excessive blood loss ratios were significantly higher in the mild thrombocytopenia group compared with the control group (P < 0.001 and P < 0.05, respectively). There was no significant difference between the mild thrombocytopenia and control groups in terms of the number of patients receiving a blood transfusion. The probability of excessive blood loss was significantly higher in the mild thrombocytopenia group, even after adjusting the odds ratio for confounding factors (adjusted odds ratio 1.94, 95% confidence interval 1.27–2.95, P = 0.002).ConclusionMild thrombocytopenia appears to increase the likelihood of peripartum hemorrhage in elective cesarean deliveries in uncomplicated pregnancies.
Post‐abortion contraceptive adoption in Ethiopia
Authors: Teshome Abel , Wondafrash Mekitie , Gashawbeza Biruck , Nigatu Balkachew , Asrat Matiyas , Compton Sarah D. 
Published: December 2020

AbstractObjectiveTo assess the effect of couple counseling on modern contraception adoption among women receiving abortions.MethodsA cross‐sectional study was conducted between October 2019 and May 2020 at the abortion clinic of Saint Paul's Hospital Millennium Medical College, Addis Ababa, Ethiopia. Women receiving abortion care were interviewed using Open Data Kit. Logistic regression was used to assess predictors of modern contraception adoption.ResultsDuring the study period, a total of 326 women receiving abortion care were interviewed and 112 (34.4%) received couple counseling. Of the 112, 89 (79.5%) adopted modern contraception. The odds of using a modern contraceptive method were 2.34 times higher among women whose partner approved (adjusted odds ratio [aOR] 2.34; 95% confidence interval [CI] 1.05–5.22) compared with those without partner approval. The odds of using a modern contraceptive method was 1.78 times higher among women who believed they had partner support (aOR 1.78; 95% CI 1.03–3.10) compared with women without support.ConclusionFew women received couple counseling for contraception. Partner approval and a woman's belief that her partner supports her contraception decision were associated with contraception adoption.