bluuebunny/crossref_metadata_2025_split huggingface dataset:
Intronic tRNAs of mitochondrial origin regulate constitutive and alternative splicing
Authors: Hoser Simon M. , Hoffmann Anne , Meindl Andreas , Gamper Maximilian , Fallmann Jörg , Bernhart Stephan H. , Müller Lisa , Ploner Melanie , Misslinger Matthias , Kremser Leopold , Lindner Herbert , Geley Stephan , Schaal Heiner , Stadler Peter F. , Huettenhofer Alexander 
Published: December 2020

AbstractBackgroundThe presence of nuclear mitochondrial DNA (numtDNA) has been reported within several nuclear genomes. Next to mitochondrial protein-coding genes, numtDNA sequences also encode for mitochondrial tRNA genes. However, the biological roles of numtDNA remain elusive.ResultsEmploying in silico analysis, we identify 281 mitochondrial tRNA homologs in the human genome, which we term nimtRNAs (nuclear intronic mitochondrial-derived tRNAs), being contained within introns of 76 nuclear host genes. Despite base changes in nimtRNAs when compared to their mtRNA homologs, a canonical tRNA cloverleaf structure is maintained. To address potential functions of intronic nimtRNAs, we insert them into introns of constitutive and alternative splicing reporters and demonstrate that nimtRNAs promote pre-mRNA splicing, dependent on the number and positioning of nimtRNA genes and splice site recognition efficiency. A mutational analysis reveals that the nimtRNA cloverleaf structure is required for the observed splicing increase. Utilizing a CRISPR/Cas9 approach, we show that a partial deletion of a single endogenous nimtRNALyswithin intron 28 of thePPFIBP1gene decreases inclusion of the downstream-located exon 29 of thePPFIBP1mRNA. By employing a pull-down approach followed by mass spectrometry, a 3′-splice site-associated protein network is identified, including KHDRBS1, which we show directly interacts with nimtRNATyrby an electrophoretic mobility shift assay.ConclusionsWe propose that nimtRNAs, along with associated protein factors, can act as a novel class of intronic splicing regulatory elements in the human genome by participating in the regulation of splicing.
Simultaneous epigenetic perturbation and genome imaging reveal distinct roles of H3K9me3 in chromatin architecture and transcription
Authors: Feng Ying , Wang Yao , Wang Xiangnan , He Xiaohui , Yang Chen , Naseri Ardalan , Pederson Thoru , Zheng Jing , Zhang Shaojie , Xiao Xiao , Xie Wei , Ma Hanhui 
Published: December 2020

AbstractIntroductionDespite the long-observed correlation between H3K9me3, chromatin architecture, and transcriptional repression, how H3K9me3 regulates genome higher-order organization and transcriptional activity in living cells remains unclear.ResultHere, we develop EpiGo (Epigenetic perturbation induced Genome organization)-KRAB to introduce H3K9me3 at hundreds of loci spanning megabases on human chromosome 19 and simultaneously track genome organization. EpiGo-KRAB is sufficient to induce genomic clustering and de novo heterochromatin-like domain formation, which requires SETDB1, a methyltransferase of H3K9me3. Unexpectedly, EpiGo-KRAB-induced heterochromatin-like domain does not result in widespread gene repression except a small set of genes with concurrent loss of H3K4me3 and H3K27ac. Ectopic H3K9me3 appears to spread in inactive regions but is largely restricted from transcriptional initiation sites in active regions. Finally, Hi-C analysis showed that EpiGo-KRAB reshapes existing compartments mainly at compartment boundaries.ConclusionsThese results reveal the role of H3K9me3 in genome organization could be partially separated from its function in gene repression.
Use of low-molecular-weight heparin in severe paraquat poisoning: a case report
Authors: Montoya-Giraldo Maria A. , Díaz Luisa F. , Gómez Ubier E. , Quintero Juliana , Zuluaga Andres F. 
Published: December 2020

Abstract
Background
Acute paraquat ingestion remains a leading cause of mortality in developing countries. There is currently no evidence that treatment with high-dose immunosuppressants and antioxidants improves survival in patients with paraquat poisoning, and better options are urgently needed. Here, we describe the unexpected survival and recovery of a patient with a potentially fatal paraquat poisoning.

Case presentation
After ingesting 28 mL of paraquat (20% ion w/v), confirmed by a deep blue color in the urine dithionite test (UDT), a 17-year-old Hispanic Colombian boy was treated according to the hospital protocol with cyclophosphamide, methylprednisolone, N-acetylcysteine, vitamin E and propranolol. Gastrointestinal endoscopy showed extensive ulceration and necrosis. As a novelty, enoxaparin at a single dose of 60 mg was added to his treatment. Despite the evidence of severe mucosal burns in the gastrointestinal tract and high paraquat concentrations found in the UDT, the clinical condition began to improve after 1 day of treatment, with full recovery and discharge from hospital after 21 days.

Conclusions
Although the amount of paraquat ingested by the patient was large and the UDT indicated severe poisoning with a somber prognosis, unexpected survival of the patient was observed, and the addition of enoxaparin was the only change from the standard treatment.
Unexpected tumor response to palliative pelvic radiotherapy in mismatch repair-deficient advanced prostate cancer: a case report
Authors: Aluisio Giovanni , Mazzeo Ercole , Lohr Frank , Fiocchi Federica , Bettelli Stefania , Baldessari Cinzia , Paterlini Maurizio , Bruni Alessio 
Published: December 2020

Abstract
Background
Mismatch-repair-deficiency resulting in microsatellite instability (MSI) may confer increased radiosensitivity in locally advanced/metastatic tumors and thus radiotherapy (RT) potentially might have a changing role in treating this subset of patients, alone or in combination with checkpoint inhibitors.

Case presentation
We report a 76 year-old Italian male patient presenting with locally advanced undifferentiated prostate cancer (LAPC), infiltrating bladder and rectum. Molecular analysis revealed high-MSI with an altered expression of MSH2 and MSH6 at immunohistochemistry. Two months after 6 chemotherapy cycles with Docetaxel associated to an LHRH analogue, a computed tomography scan showed stable disease. After palliative RT (30 Gy/10 fractions) directed to the tumor mass with a 3D-conformal setup, a follow-up computed tomography scan at 8 weeks revealed an impressive response that remained stable at computed tomography after 9 months, with sustained biochemical response. To our knowledge, this is the first case of such a sustained response to low dose RT alone in high-MSI LAPC.

Conclusions
Routine evaluation of MSI in patients with locally problematic advanced tumors might change treatment strategy and treatment aim in this setting, from a purely palliative approach to a quasi-curative paradigm.
Numerical method of highly nonlinear and nonautonomous neutral stochastic differential delay equations with Markovian switching
Authors: Gao Shuaibin , Hu Junhao 
Published: December 2020

AbstractIn this paper, we establish a partially truncated Euler–Maruyama scheme for highly nonlinear and nonautonomous neutral stochastic differential delay equations with Markovian switching. We investigate the strong convergence rate and almost sure exponential stability of the numerical solutions under the generalized Khasminskii-type condition.
Intraoperative measurements of reverse total shoulder arthroplasty contact forces
Authors: Farmer Kevin W. , Higa Masaru , Banks Scott A. , Chang Chih-Chiang , Struk Aimee M. , Wright Thomas W. 
Published: December 2020

Abstract
Purpose
Instability and fractures may result from tensioning errors during reverse total shoulder arthroplasty (RTSA). To help understand tension, we measured intraoperative glenohumeral contact forces (GHCF) during RTSA.

Methods
Twenty-six patients underwent RTSA, and a strain gauge was attached to a baseplate, along with a trial glenosphere. GHCF were measured in passive neutral, flexion, abduction, scaption, and external rotation (ER). Five patients were excluded due to wire issues. The average age was 70 (range, 54–84), the average height was 169.5 cm (range, 154.9–182.9), and the average weight was 82.7 kg (range, 45.4–129.3). There were 11 females and 10 males, and thirteen 42 mm and 8 38 mm glenospheres.

Results
The mean GHCF values were 135 N at neutral, 123 N at ER, 165 N in flexion, 110 N in scaption, and 205 N in abduction. The mean force at terminal abduction is significantly greater than at terminal ER and scaption (p < 0.05).

Conclusions
These findings could help reduce inappropriate tensioning.
Association of depression with sexual function in women with history of recurrent pregnancy Loss: descriptive-correlational study in Tehran, Iran
Authors: Azin Seyed Ali , Golbabaei Fahimeh , Warmelink J. Catja , Eghtedari Sadaf , Haghani Shima , Ranjbar Fahimeh 
Published: December 2020

Abstract
Purpose
The present study aimed to investigate the relationship between depression and sexual function in women with recurrent pregnancy loss.

Methods
In a cross-sectional correlational study, 130 consecutive patients with history of recurrent pregnancy loss were included who referred to Avicenna Fertility Center in Tehran, Iran during November 2018–February 2019. The outcomes were sexual dysfunction (Assessed with the Female Sexual Function Index) and depression (Evaluated with the Beck’s Depression Inventory). The study data were analyzed by using Mann-Whitney and Kruskal-Wallis tests.

Results
The study findings revealed that 40.8% of the participants suffered from some degrees of depression. The data analysis revealed that depression had a significant inverse correlation with sexual function and its domains (r = − 0.392, p < 0.001, R2= 0.15). The spouse’ education level and economic status demonstrated a significant relationship with women’s sexual function (p = 0.01, p = 0.033). A significant relationship was also detected between women’s depression and economic status (p = 0.028).

Conclusions
The study findings showed that women with RPL who had severe depression indicated lower score of sexual function. Since psychological and sexual problems are not reported to health care providers due to giving priority to fertility issues or considering such issues as taboos, the assessment of sexual and mental health needs to be part of the consultation in women with history of RPL, whether the patient seeks help for depression and sexual dysfunction or not.
Biomechanical analysis of skull trauma and opportunity in neuroradiology interpretation to explain the post-concussion syndrome: literature review and case studies presentation
Authors: Distriquin Yannick , Vital Jean-Marc , Ella Bruno 
Published: December 2020

AbstractTraumatic head injuries are one of the leading causes of emergency worldwide due to their frequency and associated morbidity. The circumstances of their onset are often sports activities or road accidents. Numerous studies analysed post-concussion syndrome from a psychiatric and metabolic point of view after a mild head trauma. The aim was to help understand how the skull can suffer a mechanical deformation during a mild cranial trauma, and if it can explain the occurrence of some post-concussion symptoms. A multi-step electronic search was performed, using the following keywords: biomechanics properties of the skull, three-dimensional computed tomography of head injuries, statistics on skull injuries, and normative studies of the skull base. We analysed studies related to the observation of the skull after mild head trauma. The analysis of 23 studies showed that the cranial sutures could be deformed even during a mild head trauma. The skull base is a major site of bone shuffle. Three-dimensional computed tomography can help to understand some post-concussion symptoms. Four case studies showed stenosis of jugular foramen and petrous bone asymmetries who can correlate with concussion symptomatology. In conclusion, the skull is a heterogeneous structure that can be deformed even during a mild head trauma.
The relationship between coronary artery severity and insulin resistance in patients with impaired glucose tolerance and metabolic syndrome
Authors: Metwally Yasser Gaber , Sedrak Heba Kamal , Shaltout Inas Fahiem 
Published: December 2020

AbstractBackgroundThe relation between metabolic syndrome (MS) and coronary artery disease (CAD) is multifactorial, and the well-known risk factors can explain only about 25% of the disease as the relation between insulin resistance (IR) on top of MS and severity of CAD still a subject of debate with conflicting data. Also, IR and glucose intolerance are included in the definition of metabolic syndrome only by some associations. So, the aim of this research was to analyze the the relation between CAD severity (measured by the modified Gensini score) and the different components of the metabolic syndrome including insulin resistance and glucose intolerance.ResultsOut of 70 patients enrolled, 71.4% represented impaired glucose tolerance group (IGT group;n= 50) and 28.6% represented normal glucose tolerance group (NGT group;n= 20). The following variables were significantly greater among the IGT group including BMI (30.19 ± 2.27 vs 23.62 ± 2.92;P< 0.001), waist circumference (105.09 ± 8.02 vs 92.89 ± 19.92;P< 0.001), frequency of hypertension (80% vs 50%;P= 0.012), 2 h post prandial glucose (164 ± 30 vs 110 ± 20;P< 0. 04), fasting insulin (9.2 ± 1.2 vs 5.1 ± 4.3;P= 0.003), HOMA-IR (3.03 ± 0.12% vs 1.9 ± 0.12,P< 0.001), serum uric acid (4.8 ± 1.3 vs 5.8 ± 2.6;P= 0.03), mean modified Gensini score (22.3 ± 9.2 vs 15.4 ± 6.8;P< 0.001), while the mean serum HDL was significantly higher among the NGT group (40.8 ± 10.1 vs 50.6 ± 10.2;P< 0.001). There was a positive correlation between HOMA–IR; hs-CRP; MS-related variables (including waist circumflex; BMI; serum uric acid; HDL; TG);and the modified Gensini score (for HOMA,r= 0.63;P< 0.001). Several predictors for high scores of modified Gensini were noted in multiple regression analysis; however, out of those several predictors, HOMA-IR was the strongest one (OR = 4.0, 95% CI = (2.04-7.08).ConclusionIR is an independent risk factor for CAD severity in patients with IGT and MS. In patients with IR; those with aggressive CAD (or with a positive family history of premature CAD), IGT should be managed aggressively even before any evidence of frank diabetes. IR workup should be recommended among the other standard workup for those patients, if documented, interventions targeted against IR should be considered among the other standard management.
Increasing Referrals of Patients With Gastrointestinal Cancer to a Cancer Rehabilitation Program: A Quality Improvement Initiative
Authors: Nadler Michelle B. , Rose April A.N. , Prince Rebecca , Eng Lawson , Lott Anthony , Grant Robert C. , Jones Jennifer M. , Enright Katherine 
Published: December 2020

BACKGROUND: People with cancer are at risk for initial, late, and long-term effects of cancer and its treatments. Cancer rehabilitation (CR) focuses on prevention/treatment of these sequelae and optimization of physical, social, and vocational functioning. Our center has a multidisciplinary impairment-driven outpatient CR program, but referrals of patients with GI cancer were low. AIMS: We aimed (for 2019, relative to 2018) (1) to increase CR referrals of patients with GI cancer by 50% and (2) to increase the proportion of referrals coming from oncologists. Balancing measures included inappropriate referrals and cancellations. METHODS: A rapid cycle improvement approach was used to optimize GI referrals to the CR program. Barriers to CR referral were identified through a literature review and informal interviews of GI clinicians. Barriers included (a) knowledge of CR program existence, (b) awareness of the referral process, (c) time, and (d) lack of CR program exposure. The team used Plan-Do-Study-Act (PDSA) cycles every 2 months from January to December 2019 to address barriers. A p-chart was used to analyze the results. RESULTS: PDSA cycles included CR program advertisement, a presentation to GI staff, nurse-led patient identification, patient-facing posters, and clinician thank-you emails. The p-chart showed a 100% relative increase in referral numbers and an improvement in the percentage of patients referred by oncologists from 51% to 75%. There was no significant change in inappropriate referrals or cancellations. CONCLUSION: Through PDSA cycles, we improved the total number of patients with GI cancer and percentage referred by an oncologist to a CR program. Future work will assess sustainability.
Estimating the time-varying reproduction number of SARS-CoV-2 using national and subnational case counts
Authors: Abbott Sam , Hellewell Joel , Thompson Robin N. , Sherratt Katharine , Gibbs Hamish P. , Bosse Nikos I. , Munday James D. , Meakin Sophie , Doughty Emma L. , Chun June Young , Chan Yung-Wai Desmond , Finger Flavio , Campbell Paul , Endo Akira , Pearson Carl A. B. , Gimma Amy , Russell Tim ,   CMMID COVID modelling group, Flasche Stefan , Kucharski Adam J. , Eggo Rosalind M. , Funk Sebastian 
Published: December 2020

Background:Assessing temporal variations in transmission in different countries is essential for monitoring the epidemic, evaluating the effectiveness of public health interventions and estimating the impact of changes in policy.Methods:We use case and death notification data to generate daily estimates of the time-varying reproduction number globally, regionally, nationally, and subnationally over a 12-week rolling window. Our modelling framework, based on open source tooling, accounts for uncertainty in reporting delays, so that the reproduction number is estimated based on underlying latent infections.Results:Estimates of the reproduction number, trajectories of infections, and forecasts are displayed on a dedicated website as both maps and time series, and made available to download in tabular form.Conclusions: This decision-support tool can be used to assess changes in virus transmission both globally, regionally, nationally, and subnationally. This allows public health officials and policymakers to track the progress of the outbreak in near real-time using an epidemiologically valid measure. As well as providing regular updates on our website, we also provide an open source tool-set so that our approach can be used directly by researchers and policymakers on confidential data-sets. We hope that our tool will be used to support decisions in countries worldwide throughout the ongoing COVID-19 pandemic.
DEAR (DIGITAL EXPLORATION CAREER): HYPERMEDIA-BASED INNOVATION MEDIA FOR GUINDANCE AND COUNSELING TO EXPLORE STUDENT CAREER IN THE INDUSTRIAL REVOLUTION 4.0
Authors: Aji Bayu Selo , Kurniasih Cucu , Rosiani Berlin Fanta , Bhakti Caraka Putra 
Published: December 2020

This scientific work aims to provide creative innovations in guidance and counseling services using the BK media to explore student careers. Entering the era of the Industrial Revolution 4.0, students today must have the ability and skills to predict a rapidly changing future, especially concerning career problems. Guidance and Counseling Teachers here play an essential role in developing students' potential, and one way is to optimize the role of Guidance and Counseling in schools by optimizing services about career exploration. For support services on career exploration, BK (Guidance and Counseling) media needed to improve students' understanding of the services provided. One of them uses DEAR (Digital Exploration Career) based media as an alternative and innovation that can use to explore students' careers. DEAR (Digital Exploration Career) is a hypermedia-based BK media. Each use of this media in service delivery also pays attention to students' needs, materials, entertainment, and evaluations so that the media is effective in service delivery even though sometimes without face to face. So that this media provides a new atmosphere and innovation in the provision of BK services so that they can run dynamically by the times. Then DEAR (Digital Exploration Career) Hypermedia-Based Bk Media Innovation is one solution that can use to explore students' careers in the industrial revolution era 4.0
STRATEGIES FOR DEVELOPING RELIGIOUS CULTURE TO SHAPE, THE CHARACTER OF STUDENTS
Authors: Wening Muslimah Hikmah , Hasanah Enung 
Published: December 2020

Religious culture is applied in schools so that the good habits of students, so that the strategy of developing religious culture is essential to be applied to schools so that students have a noble character in Islamic education. The purpose of this study is to describe the strategy of developing a religious culture to shape students' character. This research is qualitative. Data collection obtained through in-depth interviews, observation, and documentation studies. This study's subject was determined based on a purposive sampling technique with information from the homeroom teacher. Checking the validity of the data starts with using a check and continues with the triangulation technique. The results showed that the strategy of developing a culture of religious and improving the character of students at SD Muhammadiyah Tonggalan, 1) creating a religious atmosphere in schools including allocating half an hour to learn to read the Qur'an before the lesson begins, do the Dhuha, Dhuhr, Asr prayer, Ramadan fasting, qurban worship, hajj rituals, social services, Sunday morning teachings, also (2) habituation of Islamic values. The research findings expected to illustrate that the strategy of developing religious culture can shape students' character.
CONCEITOS CIENTÍFICOS EM SALA DE AULA: MULTIPLICIDADE DE SINAIS EM LIBRAS E POSSÍVEIS DIFICULDADES NA APRENDIZAGEM
Authors: Cruz Frederico Alan de Oliveira , Nogueira Ana Carla Ziner , Cruz Sergio Manuel Serra da 
Published: December 2020

A busca por uma educação de qualidade e inclusiva é pauta emergente nas discussões sobre a necessidade de criar uma sociedade mais justa e igualitária, assim as escolas devem ser um ambiente que possa contribuir na formação de alunos com e sem necessidades educacionais especiais (NEE). A questão é que o atualmente os modelos de ensino não são capazes de atender a heterogeneidade da sala de aula, como no caso dos estudantes surdos que tem seu desenvolvimento afetado pela falta de padronização de termos ou conceitos científicos apresentados ao longo das aulas. Isso ocorre pela falta do uso de elementos visuais ou mesmo da existência de sinais adequados as grandezas e termos científicos das diversas disciplinas e que produz impacto direto aprendizagem do que está sendo estudado. Neste trabalho apresentamos uma discussão de como este cenário pode intensificar a exclusão desses alunos e quais os possíveis caminhos são necessários para melhorar a formação de indivíduos nessa condição.
A ELEGIA EM ROMA: SEXTO PROPÉRCIO
Authors: Montagner Airto Ceolin 
Published: December 2020

A literatura latina conhece seu apogeu na época de Augusto. Entre os diversos gêneros cultivados, a elegia, que teve sua origem entre os gregos, ganhou relevância com as obras de quatro poetas, a saber, Cornélio Galo, Tibulo, Propércio e Ovídio. A produção desses poetas permite observar o conjunto da tópica elegíaca latina, principalmente no que ela tem de mais peculiar: o erotismo como experiência pessoal e biográfica do poeta. O amor é visto como paixão, razão de vida e fonte de inspiração. Amor é também doença, páthos, que toma conta do amante em todos os aspectos mais pungentes das suas contradições, ante uma senhora volúvel e incapaz de ser fiel, muito menos uma domina, no conceito da respeitabilidade romana. Sexto Propércio é o poeta elegíaco aqui considerado e através dele é possível analisar criticamente o alcance da poesia elegíaca latina.