bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cost-Effectiveness of Paclitaxel, Doxorubicin, Cyclophosphamide and Trastuzumab Versus Docetaxel, Cisplatin and Trastuzumab in New Adjuvant Therapy of Breast Cancer in China.
Authors: XU Qiaoping , Yuanyuan LI , Jiejing ZHU , Jian Liu , Qingyu LI , Lingya Chen , Ying LUO , Changchen SHI , Yangling LI , Wei YAN 
Published: June 2020

Abstract
Background: Breast cancer is the most common cancer among women in China. Amplification of the Human epidermal growth factor receptor type 2 (HER2) gene is present and overexpressed in 18–20% of breast cancers and historically has been associated with inferior disease-related outcomes. There has been increasing interest in de-escalation of therapy for low-risk disease. This study analyzes the cost-effectiveness of Doxorubicin/ Cyclophosphamide/ Paclitaxel/ Trastuzumab (AC-TH) and Docetaxel/Carboplatin/Trastuzumab(TCH) from payer perspective over a 5 year time horizon.Methods: A half-cycle corrected Markov model was built to simulate the process of breast cancer events and death occurred in both AC-TH and TCH armed patients. Cost data came from studies based on a Chinese hospital. One-way sensitivity analyses as well as second-order Monte Carlo and probabilistic sensitivity analyses were performed.The transition probabilities and utilities were extracted from published literature, and deterministic sensitivity analyses were conducted.Results: We identified 41 breast cancer patients at Hangzhou First People’s Hospital, among whom 15(60%) had a partial response for AC-TH treatment and 13 (81.25%) had a partial response for TCH treatment.No cardiac toxicity was observed. Hematologic grade 3 or 4 toxicities were observed in 1 of 28 patients.Nonhematologic grade 3 or 4 toxicities with a reverse pattern were observed in 6 of 29 patients. The mean QALY gain per patient compared with TCH was 0.25 with AC-TH, while the incremental costs were $US13,142.The incremental cost-effectiveness ratio (ICER) of AC-TH versus TCH was $US 52,565 per QALY gained.Conclusions: This study concluded that TCH neoadjuvant chemotherapy was feasible and active in HER2-overexpressing breast cancer patients in terms of the pathological complete response, complete response, and partial response rates and manageable toxicities.
Identification of important genes and pathways leading to poor prognosis of non-small cell lung cancer using Integrated Bioinformatics
Authors: Cui Shengjin , Huang Feixia , Yu Fan , Jian Shihui , Zhang Lijun , Liu Xintong , Chen Siping , Zhou Yiwen , Xiao Lijia 
Published: June 2020

Abstract
The authors have requested that this preprint be removed from Research Square.
Integrative Analysis of four miRNA prognostic signatures of prostate cancer
Authors: Chi Chen , Chen Xianwu , Yao Liping , Li Min , Xiang Lanting , Dong Xidan , Guo Xiaoling , Zhang Quanbo 
Published: June 2020

Abstract
Background

Prostate cancer (PCa) is the most common urological cancer among men, having a poor prognosis, which is hard to accurately evaluate based on the present methods. MicroRNAs (miRNAs), a class of internal non-coding small RNA, can involve in the regulation of tumor biological function. So far, many researchers have tried to explore the relationship of malignant progress of PCa with miRNA, while there are just limited studies conducting the comprehensive analysis of miRNA in PCa clinical significance.
Methods

The data of miRNA and mRNA expressions in PCa were downloaded from TCGA database, and were performed the overall survival (OS) analysis using Survival package of R software to harvest the differentially expressed miRNAs (DEMs) and differentially expressed genes (DEGs). The bioinformatics tools such as TargetScan, miRDB, and miRanda were also conducted to forecast the desired target genes related with prognostic DEMs. In addition, both GO and KEGG analyses were used to uncover the fundamental signaling pathways and cellular processes in PCa as well as the protein-protein interaction (PPI) network was constructed through STRING and Cytoscape software.
Results

Firstly, 4 DEMs (miR-19a-3p, miR-144-3p, miR-223-5p, and miR-483-3p) were found having significantly associated with overall survival in PCa. Based on the criteria with FDR < 0.05 and |log2FC| > 1, 33 genes were screened out as DEGs. Besides, the functional enrichment analysis revealed that these DEGs of 4 miRNAs may participate in cancer-related pathways like FoxO and PI3K-Akt signaling pathway. Lastly, the low expression of CD177 may be potentially associated with poor survival of patients in PCa.
Conclusion

This study systematically analyzed multiple PCa prognostic DEMs (miR-19a-3p, miR-144-3p, miR-223-5p, and miR-483-3p), and verified a novel DEG signature (CD177) that can be used to effectively assess the prognosis of PCa patients.
Triglycerides to total cholesterol ratio: an early screening tool for NAFLD in Chinese populations
Authors: Chen Jingyuan , Yang Yiping , Yang Qize , Wang Jiangang , Chen Zhiheng , Yuan Hong , Lu Yao 
Published: June 2020

Abstract
Background

Non-alcoholic fatty liver disease(NAFLD) has a high prevalence in the general population worldwide. Both triglycerides (TG) and total cholesterol (TC) are correlated with the prevalence of NAFLD. The study purpose is to determine whether TG/TC is an effective method to screen NAFLD in the general Chinese population.
Methods

93,449 subjects with average age of 43.7 ± 14.3 years were included in this cross-sectional study. Multiple logistic regressions and receiver operator characteristic curve (ROC) analyses were performed.
Results

Among these subjects, 16,138 (42.7%) men and 9,591 (17.2%) women were diagnosed with NAFLD. Subjects in the higher quartiles of TG/TC had a higher prevalence of NAFLD. After adjusting multiple confounding factors, the odds ratio (OR) for NAFLD in the highest compared with the lowest quartile was 4.08 (95%CI 3.64, 4.57) in men and 4.65 (95%CI 4.14, 5.21) in women. Moreover, ROC analyses suggested TG/TC showed high diagnostic ability for detecting NAFLD, and the areas under the curves (AUC) in men and women were 0.920 (95%CI 0.917, 0.923) and 0.863 (95%CI 0.859, 0.867), respectively. Furthermore, the diagnostic ability was significantly higher in younger age groups. The AUC in 18–34 and 35–44 years group were 0.943 (95%CI 0.939, 0.946) and 0.921 (95%CI 0.917, 0.925), respectively. Besides, the women and the young people had a greater negative predictive values (90.84% for women, 93.13% for aged 18–34).
Conclusion

TG/TC shows a high diagnostic accuracy for identifying NAFLD in young women, providing an important clue to establish a new tool to screen NAFLD particularly in Chinese young females.
Declined GDCA is Correlated With Adverse Clinical Outcome For Gestational Diabetes Mellitus
Authors: Zhu Bo , Ma Zhixin , Zhu Yuning , Fang Lei , Zhang Hong , Kong Hongwei , Dajing Xia 
Published: June 2020

Abstract
BackgroundGestational diabetes mellitus (GDM) is characterized by glycemia and insulin disorders. Bile acids (Bas) have emerged as vital signaling molecules in glucose metabolic regulation. Bas change in GDM are still unclear, it exerts great significance to illustrate the change of Bas in GDM.MethodsWe organized GDM patient (n = 67) and normal pregnant women (n = 48) around the oral glucose tolerance test (OGTT) screening period, fasting serums were collected for the measurement of Bas. Clinical data were collected and Bas metabolism profiles were analyzed in GDM and normal glucose tolerance (NGT). Delivery characteristics, delivery gestational age and infant birthweight were abstracted from medical record.ResultsGDM patients exert the distinctive features compared with NGT, including higher BMI, glucose, insulin (both fasting and OGTT) and HbA1c levels. GDM also gained higher insulin resistance index (HOMA-IR) and decreased β-cell compensation (DIo). Total bile acids (TBA) remained stable, but glycodeoxycholic acid (GDCA) and taurodeoxycholic acid (TDCA) declined significantly in GDM. GDCA was inversely correlated with HOMA-IR and positively correlated with DIo. There exists no obvious difference in clinical outcome between GDM and NGT. However, GDM patients with high HOMA-IR and low DIo are inclined to suffering higher cesarean delivery rate and earlier delivery gestational age.ConclusionsGDCA could be a valuable biomarker to evaluate insulin resistance and DIo, decreased GDCA predicts worse clinical outcome of GDM.
Predictive effect of the difference of&nbsp;brachial artery peak velocity during the valsalva maneuver in different positions on supine hypotension syndrome of parturient after spinal anesthesia
Authors: Qu Min , Liu Tianlin , Li Jing , Yu Lili 
Published: June 2020

Abstract
BACKGROUND:The effectiveness of predicting the incidence of supine hypotension syndrome (SHS) after spinal anesthesia was measured by ultrasonic measurement of the variation of brachial artery peak velocity in different positions of parturient. METHODS: Parturient scheduled for elective cesarean section. ASAI or Ⅱ, were divided into SHS group and no SHS group (SBP in the upper extremity decreased by>30 mmHg or decreased to<80 mmHg) after spinal anesthesia. To record HR, SBP, DBP, MAP of supine position and left lateral position before anesthesia, and to measure brachial artery peak velocity during the Valsalva maneuver by Ultrasonic. Calculate the differences of the above indexs before and after the transformation position. The receiver operating characteristic (ROC) curve was plotted to evaluate the predictive effect of each index on SHS after spinal anesthesia. RESULTS:Among the 84 patients, 41 cases (48.81%) developed SHS after spinal anesthesia. brachial artery peak velocity maximum (Vpmax), brachial artery peak velocity minimum (Vpmin) and brachial artery peak velocity variation (ΔVp) were different before and after the transformation position. The difference in SHS group is significantly greater than non SHS group (P<0.05). The areas under ROC curve (AUC) of ΔVpmin, ΔΔVp, ΔVpmax, were 0.859, 0.794，0.614, 95%CI were 0.776～0.941, 0.689～0.899, 0491～0.736, and the cut-off values were 10.55cm/s, 5.98%, 8.40cm/s. CONCLUSION:The difference of brachial artery peak velocity measured by ultrasonic in different positions of parturient during the Valsalva maneuver can effectively predict the occurrence of SHS, in which ΔVpmin≥10.55cm/s has better predictive effect.Trial registration: China Clinical Trial Register ChiCTR1900022704, prospective registered on 23. April 2019.
Systematical assessment of cancer stemness traits via one-class logistic regression (OCLR) algorithm and associations with tumor immune infiltrates in prostate cancer based on seven cohorts
Authors: Chen Hongxi , Xie Jinliang , Jin Peng 
Published: June 2020

Abstract
Background: Prostate cancer stemness (PCS) cells have been reported to drive tumor progression, recurrence and drug resistance. However, there is lacking systematical assessment of stemness traits and associations with immunological properties in prostate adenocarcinoma (PRAD). Methods We collected 7 PRAD cohorts with 1465 men and calculated the stemness indices for each sample using the innovative one-class logistic regression (OCLR) machine learning algorithm. We selected the mRNAsi to quantify the stemness traits that correlated significantly with prognosis and accordingly identified 21 PCS-related CpG loci and 13 pivotal signature. Meanwhile, we conducted consensus clustering and classified the total cohorts into 5 PCS clusters with distinct outcomes based on the 13-gene panel. Additionally, we implemented the CIBERSORT algorithm to infer the differential abundance across 5 PCS clusters. Lastly, we used the Connectivity Map (CMap) resource to screen potential compounds for targeting PRAD stemness. Results: The 13-gene based PCS model possessed high predictive significance for progression-free survival (PFS) that was trained and validated in 7 independent cohorts. We found that PCScluster5 possessed the highest stemness fractions and suffered from the worst prognosis. Immune infiltration analysis shows that the activated immune cells (CD8+ T cell and dendritic cells) infiltrated significantly less in PCScluster5 than other clusters, especially PCScluster1, supporting the negative regulations between stemness and anticancer immunity. High mRNAsi was also found to be associated with up-regulation of immunosuppressive checkpoints, like PDL1. Finally, several potential compounds, including the top hits of cell cycle inhibitor and FOXM1 inhibitor, were identified for targeting PRAD stemness. Conclusion: Our study comprehensively evaluated the PRAD stemness traits based on large cohorts and established a 13-gene based classifier for predicting prognosis or potential strategies for stemness treatment.
The Rna-binding Protein GRSF1 Promote shepatocarcinogenesis via competitively Binding Toyy1 mRNA with mIR-30e-5p
Authors: Han Lili , Wang Xiaofei , Wu Fei , Huang Chen , Zhang Shuqun , Wang Lumin 
Published: June 2020

Abstract
Background:Numerous studies have highlighted that dysregulation of RNA binding protein (RBP) expression is causally linked with human cancer tumorigenesis. The detailed biological effect and underlying mechanisms of RBP GRSF1 in hepatocellular carcinoma (HCC) remain unclear.Methods:GRSF1 expression in HCC tissues and cells was measured by western blotting and qRT-PCR assays. HCC cells with stable knockdown of GRSF1 were established using two sh-RNA encoding lentiviruses. Then the functions of GRSF1 in HCC were explored using MTT, colony formation, flow cytometry, Transwell assays and xenograft model. Transcriptometric sequencing in GRSF1-deficient MHCC-97H cells were carried out to identify the downstream effector of GSRF1. The regulatory mechanisms among GRSF1, YY1 and miR-30e-5p were investigated by RNA immunoprecipitation, luciferase assay, RNA pull down and ChIP assay.Results:GRSF1was frequently increased in HCC tissue and cells with a worse clinical outcomes in HCC. GRSF1 worked as a novel oncogenic RBP in vitro and in vivo via post-regulating the stability of YY1 mRNA. And the GUUU motifs on YY1 3`UTR 2663-2847 was the specific binding motifs for GRSF1. More interesting, YY1 feedback promoted GRSF1 expression by binding to GRSF1 promoters. In addition, YY1 was a critical target of miR-30e-5p, which was confirmed by our data that inhibited HCC hepatocarcinogenesis. Further investigation showed GRSF1 and miR-30e-5p competitively regulated YY1 via binding to its YY1 3`UTR 2663-2847 region. At last, we identified that 3-amino-6-(4-methylsulfonylphenyl)-N-phenylpyrazine-2-carboxamide (VE821) blocked HCC progression by inhibiting the GRSF1/YY1 pathway.Conclusions:GRSF1 promoted hepatocarcinogenesis via competitively binding to YY1 3`UTR 2663-2847 with miR-30e-5p.The interaction network among GRSF1, YY1 and miR-30e-5p provided a new insight for the HCC pathogenesis, and VE821 may serve as a novel agent with potential for HCC treatment via inhibiting GRSF1/YY1 axis.
Efficacy and Outcomes of a Novel Telephone-based Gout Disease Management Program
Authors: Harash Abdalhamid Al , Sharma Tarun , Laginya Gina , Wasko Mary Chester M , Ayoub William T 
Published: June 2020

Abstract
Objective: Gout is the most common inflammatory arthritis in the United States. Despite published guidelines, management remains suboptimal, leading to unnecessary morbidity and increased cost of care. We have designed the gout disease management program (GDMP) to improve outcomes, increase patient satisfaction and decrease healthcare utilization.Methods: Gout patients were seen at their usual rheumatology clinical visit and offered participation in the GDMP. Data were collected between April 2017 and May 2019. Serum uric acid (SUA) levels were measured at initial outpatient encounter, at entrance to GDMP and every 4 weeks until SUA was at goal of ≤6 mg/dl. During telephone encounters, gout-related recent hospitalizations and ER or urgent care visits since last encounter were ascertained. Self-reported gout medication usage and adherence were also determined. Patient satisfaction with GDMP was surveyed using a 5-point Likert scale.Results: A total of 158 patients were enrolled, of which 112 had ≥ 1 telephone encounter and were included in our analyses. During the telephone phase, 79 patients (70%) achieved SUA goal of ≤6.0 mg/dl, while90 patients (80%) achieved an acceptable SUA of ≤6.5 mg/dl. Only 3 patients (2.6%) required hospitalization or visits to an ER or urgent care center due to gout flare, and 98% rated their encounter as a 5 on the 5-point Likert scale.Conclusion: Our telephone-based management program for gout led to improved clinical outcomes as defined by the ACR guidelines, decreased healthcare visits, and had high patient satisfaction.
Application of Intelligent Orienteering Based on Internet of Things
Authors: Zhang Donghui , Liu Ruijie 
Published: June 2020

Abstract
Orienteering has gradually changed from a professional sport to a civilian sport. Especially in recent years, orienteering has been widely popularized. Many colleges and universities in China have also set up this course. With the improvement of people's living conditions, orienteering has really become a leisure sport in modern people's life. The reduced difficulty of sports enables more people to participate, but it also exposes a series of problems. As the existing positioning technology is relatively backward, the progress in personnel tracking, emergency services and other aspects is slow. To solve these problems, a new intelligent Orienteering application system is developed based on the Internet of things. ZigBee network architecture is adopted in the system. ZigBee is the mainstream scheme in the current wireless sensor network technology, which has many advantages such as convenient carrying, low power consumption, and signal stability. Due to the complex communication environment in mobile signal, the collected information is processed by signal amplification and signal anti-interference technology. By adding anti-interference devices, video isolators and other devices, the signal is guaranteed to the maximum extent. In order to verify the actual effect of this system, through a number of experimental studies including the relationship between error and traffic radius and the relationship between coverage and the number of anchor nodes, the data shows that the scheme studied in this paper has a greater improvement in comprehensive performance than the traditional scheme, significantly improving the accuracy and coverage. Especially the coverage is close to 100% in the simulation experiment. This research has achieved good results and can be widely used in Orienteering Training and competition.
Characterisation of Defluorination Reaction and Solid Electrolyte Interphase in Fluoride-Shuttle Battery Anode MgF2
Authors: Kobayashi Shunsuke , Nakamoto Hirofumi , Yokoe Daisaku , Kuwabara Akihide , Abe Takeshi , Ikuhara Yuichi 
Published: June 2020

Abstract
Fluoride-shuttle batteries using fluoride ion transfer have been extensively investigated for producing post-lithium-ion batteries. One of the key issues hampering the development is the low capacity utilisation rate in anodes, causing a significant reduction in battery performance. To improve the utilisation rate, it is necessary to clarify the unexplained parts regarding the (de)fluorination behaviour to optimise the electrode design. Here, we demonstrated the characterisation of Mg metal formations and the solid electrolyte interphase (SEI) in defluorinated MgF2 anode. Mg was mainly formed in the region with electron and F ion conductivities, implying that these conductivity paths must be efficiently increased to further improve the utilisation rate. Nanosized Mg metals were formed even in the region with poor electron conductivity, implying that an efficient (de)fluorination process could be achieved by designing the electrode configuration or/and elemental composition. The influence of SEI in battery performance have currently been neglected because the problems of low utilisation rate are more serious. However, as research progresses, the control of the SEI composition and its properties should be an important investigation to further improve fluoride-shuttle battery performances.
Stable Helicene Radicals: Synthesis, Structure, Physical Properties, and Photocatalysis
Authors: Shaikh Aslam , Hossain Md Mubarak , Moutet Jules , Veleta Jose , Bloch Jan , Astashkin Andrei , Gianetti Thomas 
Published: June 2020

Abstract
Stable organic radicals have gained considerable attention in the fields of catalysis and material sciences. In particular, helical molecules are of great interest in the development and application of novel organic radicals in optoelectronic and spintronic materials. Here we report the syntheses of highly stable neutral quinolinoacridine radicals by chemical reduction of their quinolinoacridinium cation analogs. The crystal structures of these [4]helicene radicals were determined by X-ray diffraction. Electron paramagnetic resonance (EPR) measurements, supported by density functional theory (DFT) calculations, indicate that the unpaired electron is mostly localized, showing more than 40% of spin density located at the central carbon of the [4]helicene radicals. Quantitative conversion from neutral radical to cation is observed upon exposure to air, monitored via UV-vis spectroscopy. The successful photoreductive dehalogenation of aryl halides occurs in the presence of 10 mol% of [4]helicene radical under blue light.
Differences in the Microbial Composition of Hemodialysis Patients Treated With and Without β-Blockers
Authors: Lin Yi-Ting , Lin Ting-Yun , Hung Szu-Chun , Liu Po-Yu , Hung Wei-Chun , Tsai Wei-Chung , Tsai Yi-Chun , Delicano Rachel Ann , Chuang Yun-Shiuan , Wu Ping-Hsun , Kuo Mei-Chuan , Chiu Yi-Wen 
Published: June 2020

Abstract
Background:

β-blockers are commonly prescribed medications to treat cardiovascular disease and prevent sudden cardiac death during hemodialysis. Beyond the medication effects on the host, no study has investigated the impact of β-blocker on the gut microbiota in hemodialysis patients.
Results:

The β-blocker users had a higher proportion of diabetes mellitus, hypertension, dyslipidemia, coronary artery disease, heart failure, cerebrovascular disease, and concurrent medications than non-users. After propensity score (PS) matching, there were no differences in comorbidities and concomitant medications. The α-diversity (Simpson index) increased in β-blocker users with a distinct β-diversity (Bray-Curtis Index) compared to non-users in the full cohort and PS-matched cohort. In the linear discriminative analysis effect size analysis and zero-inflated Gaussian fit model, there was a significant enrichment in the genus Flavonifractor in β-blocker users compared to non-users in the full cohort and PS-matched cohort; this was confirmed by random forest analysis.
Conclusions:

Hemodialysis patients using β-blocker used had a different gut microbiota composition compared to non-users, in particular, the Flavonifractor genus was significantly increased with β-blocker treatment. These findings highlight the significant impact of β-blockers on the gut microbiome in hemodialysis patients. Further research is warranted regarding the mechanisms and their clinical consequences.