bluuebunny/crossref_metadata_2025_split huggingface dataset:
Immunoinformatic approach to the design of a novel multi-epitope vaccine against Leishmania major fused to human IgG-Fc
Authors: Fadaie Mahmood , Shahmoradi Zabihollah , Khanahmad Hossein 
Published: December 2024

Background and purpose:
Cutaneous leishmaniasis poses significant health and socioeconomic challenges, making vaccine development a top priority, especially in endemic regions. Cysteine proteases, KMP-11, and HASPB proteins are promising candidates for leishmaniasis vaccine development owing to their immunogenic properties and capacity to provoke robust immune responses, as evidenced by different investigations. This study aimed to design a recombinant chimeric protein (MEV-Fc) vaccine using multi-epitopes from these Leishmania major proteins.

Experimental approach:
The antigens were subjected to immunoinformatic prediction and screening of HTL, CTL, and B-cell epitopes. The multi-epitope protein was designed with significantly high-scoring epitopes and suitable linkers. Natural adjuvants were then added to enhance immunogenicity. Vaccine potency was innovatively improved by covalently fusing human IgG1 Fc with multi-epitope protein. To investigate how the MEV-Fc vaccine interacts with Toll-like receptors, molecular docking, multi-scale normal mode analysis simulation, and computational immune simulation were employed to study humoral and cellular immune responses.

Findings/Results:
The results demonstrated the vaccine’s antigenicity, stability, and nontoxicity. The structural validation confirmed the accuracy of the 3D models, indicating robust interactions with TLR2 and TLR4, with binding free energies of -1269.9 and -1128.7 (kcal/mol), respectively. Immune simulation results showed significant increases in IgM and IgG antibody levels following three vaccinations, along with enhanced activation of B cells, helper T cells, and cytotoxic T lymphocytes.

Conclusion and implications:
These findings provide novel insights for developing effective candidates for cutaneous leishmaniasis vaccines. However, laboratory experiments are necessary to evaluate its protective effects.
Antiaging properties of chlorogenic acid through protein and gene biomarkers in human skin fibroblast cells as photoaging model
Authors: Girsang Ermi , Ginting Chrismis N. , Lister I Nyoman Ehrich , Widowati Wahyu , Yati Afif , Widya Kusuma Hanna Sari , Azis Rizal 
Published: December 2024

Background and purpose:
Chlorogenic acid (CA) is a natural chemical that promises antiaging activity against photoaging skin damage. This research examined CA activities in mitigating skin photoaging.

Experimental approach:
UV-exposed human skin fibroblast cells were subjected to CA at 6.25, 12.5, and 25 μg/mL. The protein levels of cell secretion, such as cyclooxygenase (COX)-2, nitric oxide (NO), and interleukin (IL)-6 were measured using ELISA and colorimetry methods. Meanwhile, the mRNA expressions of glutathione peroxidase (GPX)-1, tissue inhibitor metalloproteinase (TIMP)-1, matrix metalloproteinase (MMP)-1, caspase (CASP)-3, CASP-8, and fibroblast growth factor (FGF)-2 were quantified using the qRT-PCR method.

Findings/Results:
CA treatment reduced inflammatory and aging biomarkers. CA at 6.25 μg/mL lowered NO, COX-2, and IL-6 levels to 89.44 μmol/L, 8.10 ng/mL, and 62.75 pg/mL, respectively. CA at 25 μg/mL resulted in the most significant down-regulation of MMP-1, CASP-3, and CASP-8 genes’ expression (3.27, 1.25, and 3.59, respectively). Furthermore, treatment with CA at 25 µg/mL demonstrated the most notable activity in up-regulating antioxidant markers, specifically GPX-1, and extracellular matrix (ECM) integrity markers, including TIMP-1 and FGF-2 genes’ expression.

Conclusion:
CA imposes its anti-aging activity by decreasing inflammatory and aging biomarkers, and increasing cellular antioxidant and ECM integrity.
Enhancement effect of urea toward electroporation-mediated plasmid transfection efficiency in the HEK-293 cell line
Authors: Mowla Mahshid , Gorji-Bahri Gilar , Moghimi Hamid Reza , Hashemi Atieh 
Published: December 2024

Intracellular delivery is crucial in biological and medical studies. Although many molecular tools have been created for cell-based gene therapies, it remains challenging to introduce external molecules into cells. As one of the most popular non-viral transfection methods, electroporation induces transient pores in the cell membrane by applying an external electric field. Unsatisfactory transfection efficiency and low cell viability are the major drawbacks of electroporation. To overcome these issues, the current study investigated the effect of urea on electroporation-mediated transfection efficiency.

Experimental approach:
Three voltages of electroporation, including 100, 120, and 140 V, and 3 concentrations of urea buffer, including 0.25%, 0.5%, and 1% W/V, were considered as variables in this study. The HEK-293 cell line was used for transfection, and green fluorescent protein (GFP) expression was evaluated using flow cytometry and fluorescence microscopy.

Findings/Results:
The results showed that the combination of electroporation and urea increased electroporation efficacy, but the effect depended on voltage and urea concentration. When different concentrations of urea were added to HEK-293 cells at a voltage of 100 V, the number of cells transfected by pEGFP-N1 increased (from 12.3 ± 0.2% in untreated cells to 17.35 ± 0.55%, 23.3 ± 0.3%, and 14 ± 0.1% at urea concentrations of 0.25%, 0.5%, and 1% W/V, respectively). The electroporation buffer containing 0.5% W/V urea showed the highest EGFP expression (23.3 ± 0.3%) and high cell viability (over 90%).

Conclusion and implications:
This research offers a new perspective for improving gene transfection efficiency once electroporation is utilized.
Multiple ligands simultaneous molecular docking and dynamics approach to study the synergetic inhibitory of curcumin analogs on ErbB4 tyrosine phosphorylation
Authors: Aman La Ode , Ischak Netty Ino , Tuloli Teti Sutriyati , Arfan Arfan , Asnawi Aiyi 
Published: December 2024

Background and purpose:
Lapatinib (FMM) and 5-fluorouracil (5-FU) are anticancer drugs employed in a combination approach. FMM inhibits tyrosine phosphorylation of ErbB4 while 5-FU inhibits cell proliferation. This research aimed to investigate the potential of two compounds, namely (1E,4E)-1,5-bis (4-hydroxyphenyl) penta-1,4-dien-3-one (AC01) and (1E,4E)-1,5-bis (3,4-dihydroxy phenyl) penta-1,4-dien-3-one (AC02), both as individual inhibitors and combination partners with FMM, targeting ErbB4 inhibition. AC01 and AC02 were combined with FMM, which targets ErbB4. The combination of 5-FU with FMM served as a reference in this study.

Experimental approach:
The research utilized computational simulation methods such as single and multiple ligands simultaneously docking and dynamics. Data analysis was performed using AutoDockTools and gmx_MMPBSA.

Findings/Results:
Single docking results indicated that 5-FU exhibited the lowest binding affinity, while FMM demonstrated the highest. Simultaneous docking of AC01 and AC02 paired with FMM revealed their binding positions overlapping with the FMM-5-FU workspace. The FMM-AC01 and FMM-AC02 complexes exhibited slightly weaker binding affinities compared to FMM-5-FU. In combination with FMM, AC01 and AC02 occupied the ErbB4 activation loop, whereas 5-FU was outside the activation loop. Furthermore, in their interaction with ErbB4, AC02 exhibited slightly stronger binding than AC01, as confirmed by the average binding free energy calculations from molecular dynamics simulations.

Conclusion and implications:
In conclusion, computational simulations indicated that both AC01 and AC02 have the potential to act as anticancer candidates, demonstrating ErbB4 inhibitory potential both as individual agents and in synergy with FMM.
In vitro and in vivo evaluation of anti-inflammatory activities of ethanol extract from Lom-Am-Ma-Pruek remedy for pain relief
Authors: Itharat Arunporn , Thongmee Pun , Piwngam Krit , Inprasit Janjira , Makchuchit Sunita , Kuropakornpong Pranporn , Davies Neal M. 
Published: December 2024

Background and purpose:
Lom-Am-Ma-Pruek (LAMP) remedy has been used in Thai traditional medicine to relieve pain associated with the inflammatory process. The anti-inflammatory activity and bioactivity of LAMP in an animal model have not been previously investigated. We evaluated the in-vitro and in-vivo anti-inflammatory activity of LAMP ethanol extract.

Experimental approach:
The anti-inflammatory activity of LAMP and its plant ingredients were investigated on lipopolysaccharide-stimulated NO, PGE2, and TNF-α release from RAW264.7 cells. Furthermore, the stability of LAMP under biological and chemical accelerated conditions was evaluated using the Griess reaction assay and HPLC. Lastly, rat models with ethyl phenylpropionate (EPP)-induced ear edema and carrageenan-induced paw edema were utilized to assess anti-inflammatory activity.

Findings/Results:
LAMP possessed potent inhibitory effects on NO, PGE2, and TNF-α production with IC50 values of 24.90 ± 0.86, 4.77 ± 0.03, and 35.01 ± 2.61 µg/mL, respectively. In addition, LAMP extract demonstrated stable biological activity, anti-inflammatory effects, and phytochemical content stability under stress conditions. Additionally, 0.5%, 1%, and 2% w/v LAMP significantly inhibited EPP-induced rat ear edema over time equivalent to 5% w/v phenylbutazone. LAMP at 180, 375, and 750 mg/kg also considerably reduced carrageenan-induced rat paw edema 2 h after carrageenan administration compared to phenylbutazone at 250 mg/kg.

Conclusion and implications:
LAMP has anti-inflammatory activity by inhibiting PGE2 formation. These findings are consistent with the efficacy and traditional use of the LAMP remedy in treating inflammatory diseases.
Exploring the inhibitory potential of xanthohumol on MEK1/2: a molecular docking and dynamics simulation investigation
Authors: Gholizadeh Siahmazgi Zohreh , Irani Shiva , Ghiaseddin Ali , Soutodeh Fereshteh , Gohari Zahra , Afifeh Jaber , Pashapouryeganeh Amirreza , Samimi Hilda , Naderi Mahmood , Fallah Parviz , Haghpanah Vahid 
Published: December 2024

Background and purpose:
Xanthohumol (Xn), a small molecule found in Humulus lupulus, has shown promise as an anti-cancer compound. This in silico study was performed to understand the mechanism of action of Xn as a natural compound on MEK1/2 by simulation.

Experimental approach:
After ligand and protein preparation, the best binding energy was determined using Autodock 4.2. Additionally, molecular dynamics simulations of the MEK1/2-Xn and BRaf-MEK1/2-Xn complexes were conducted using GROMACS 2022.1 software and compared to the complexes of MEK1/2-trametinib (Tra) and BRaf-MEK1/2-Tra.

Findings/Results:
The docking results revealed that the best binding energies for MEK1-Xn (-10.70 Kcal/mol), MEK2-Xn (-9.41 Kcal/mol), BRaf-MEK1-Xn (-10.91 Kcal/mol), and BRaf-MEK2-Xn (-8.54 Kcal/mol) were very close to those of the Tra complexes with their targets, MEK1 and MEK2. Furthermore, Xn was found to interact with serine 222 at the active site of these two kinases. The results of the molecular dynamics simulations also indicated that Xn induced changes in the secondary structure of the studied proteins. The root mean square of proteins and the mean radius of gyration showed significant fluctuations.

Conclusion and implications:
The findings of the study suggested that Xn, as a novel bioactive compound, potentially inhibits the MEK1/2 function in cancer cells.
“Wild” languaging
Authors: Korpinen Kaisa 
Published: December 2024

A growing body of research has illustrated that embodied and arts-based approaches can holistically support additional language learning. However, more research on the implementation of such approaches is needed to impact pedagogical practices. This study explores how integrating language and dance can create possibilities for embodied language learning in early additional language education. It is based on a project that combined dance with early Swedish language learning in a Finnish primary school. The analytical focus is on animal-themed language and dance integrated activities held when the participating pupils were in second grade. The study draws theoretically on socio-material approaches to language education, which involves considering languaging an activity that engages the entire human being and relates them to other people, materials, and spaces. The data include video recordings of lessons, lesson plans, and the researcher’s embodied experiences and written reflections. An arts-based and post-qualitative research approach is used to analyse and present languaging events. The results indicate that children were activated in languaging through communicative movement exploration that involved children transforming into or becoming animals. The languaging unfolded in unpredictable or “wild” ways in activities that involved repetition, variation, and sense-making with creative movement and sound. Children’s agency in languaging was performed collectively in relation to bodies and materialities of the space in an embodied and creative activity involving peer collaboration and performing. To conclude, the study proposes “wild” languaging as a practice of embodied language learning through dance, and discusses its pedagogical potential for early additional language education.
Authenticity in Relationships: A Moderating Factor in Stress Communication and Marital Satisfaction during the First Five Years of Marriage
Authors: Pambudi Phoebe Ramadina , Ratnasari Yudiana 
Published: December 2024

The first five years of marriage are filled with role transitions in an individual's life. Therefore, dyadic coping strategies, especially stress communication, are important for maintaining marital satisfaction. Personality traits such as authenticity also play a role in this dynamic. Authenticity affects how individuals are able to communicate openly about their stress, thereby also affecting how they feel about their marriage. Participants in this study are 1228 Indonesian citizens in the first five years of marriage. Stress communication was measured by Stress Communication dimension in Dyadic Coping Inventory (DCI), Authenticity was measured with Kernis-Goldman Authenticity Inventory Short Form (KGAI-SF), and Marital satisfaction was measured using Revised Dyadic Adjustment Scale (RDAS). Interestingly, the moderation analysis shows that authenticity plays a significantly negative moderating role in the relationship between stress communication and marital satisfaction in the first five years of marriage. This suggests that when individuals become too authentic in their relationship, the positive effect of stress communication on marital satisfaction is weakened.
Cycle of Dreams
Authors: Weiskott Eric 
Published: December 2024

An experimental hybrid work, Cycle of Dreams pairs translation and original poetry. The translations, or adaptations, are of William Langland’s strange and wild fourteenth-century dream vision, Piers Plowman, a politically radical English and Latin poem written in the wake of plague and divided into a prologue and twenty passūs or steps. Eric Weiskott transposes the action from London and Worcestershire to New England and Long Island. The translations refashion and modernize Piers Plowman by disarticulating its continuous shape and rearticulating it as a collection of lyrics. The translation appears on the left and original poetry on the right in each page opening, so that the fourteenth and twenty-first centuries speak to one another as in a dream.
Like Piers Plowman itself in manuscript culture, Cycle of Dreams attracts paratexts. Images illustrate the absent presence of Langland’s authorship. A series of glosses or marginal notes grounds the poems in critical theory, etymologies, lyric reminiscences, and statistics reflecting the desperation of our economic moment. An “oneirography” or dreamed bibliography names some of the scholarship that supports study of Piers Plowman today and some other sources for Langlandian fever dreams.
Langland can address us today, not in the voice of a bygone author whose “context” must be arduously rearticulated in the laboratories of scholarly endeavor, but one whose utopian vision is in its broad outlines no less urgent in 2024 than it was in 1381, when English rebels used Langland’s title figure as a rallying cry for insurrection. Cycle of Dreams unearths “buried dreams / of a future adequate to the present tense.”
Arte, sexualidad y vida: aprendiendo a tocar mi cuerpo en mujeres embarazadas
Authors: Montiel Marenco Wendy Yessenia , Herrera Funes William José 
Published: December 2024

El artículo “Arte, sexualidad y vida: aprendiendo a tocar mí cuerpo” presenta un proyecto educativo sociocultural en la Casa Materna Maurita Ríos, Nueva Guinea, dirigido a mujeres embarazadas. El objetivo del estudio es explorar las experiencias y percepciones de las mujeres sobre las prácticas sexuales durante el embarazo, analizando cómo afectan su bienestar físico, emocional y social. El proyecto también busca que, a través del arte, las mujeres embarazadas experimenten autocuidado, relajación, seguridad y sensibilidad, mejorando su calidad de vida durante esta etapa. Utilizando una metodología cualitativa basada en observación etnográfica, el estudio diseñó una estrategia innovadora que integra el arte en la educación sexual. A través de actividades artísticas y educativas, se busca empoderar a las mujeres y fomentar prácticas saludables en sus relaciones sexuales. Los resultados muestran que estas actividades socioculturales sensibilizaron a las participantes, ayudando a romper el tabú sobre la sexualidad durante el embarazo. Se propuso una iniciativa creativa, con una duración de un mes y cinco días, dirigida a grupos de mujeres embarazadas y autoridades, logrando una participación e interés por aprender sobre sexualidad. Finalmente, se plantea la expansión de estas estrategias a otros grupos de embarazadas y la creación de indicadores para medir el impacto de futuras iniciativas en salud sexual.
Language as a Tool of Resistance:Language Policy and Practice in the Postcolonial Period of South America
Authors: Ma Yifei 
Published: December 2024

This paper explored how language policies and practices(LPP) has become the tool of resistance in the postcolonial period of time in South America countries.With the independence of South America, the old rules about statement faced new challenges. New rules were created to support indigenous languages and indigenous cultures, trying to counter the effects of colonial history and promote national identity. This paper looks up at the language policies used in some South American nations and explores how these programs attempt to change or increase the impact of nations that have experienced colonial rule. The report shows how these language policies work and the problems they create, especially in areas like training and national publications. It also looks at how native language help people express themselves and avoid problems, particularly in marginalized communities. Finally, the paper discusses how these language policies affect geographical diversification and the many language spoken, especially in the context of globalization. This paper explified that language is not only a method for people to talk to each other, but also meaningful in creating social organizations and national brands.
Using Bloom’s Taxonomy to Evaluate HOTS in the Tasks of the Chinese Grade 7 English Textbook
Authors: Hong Yi 
Published: December 2024

This study evaluates the cognitive demands of tasks in the Chinese Layman’s Edition Grade 7 English textbook using Bloom’s Revised Taxonomy. The primary objective is to assess the distribution of lower-order thinking skills (LOTS) and higher-order thinking skills (HOTS) across the textbook’s tasks. Six cognitive categories were identified through a content analysis of 224 tasks: Remembering, Understanding, Applying, Analyzing, Evaluating, and Creating. The findings show that just 26.7% of activities concentrate on HOTS, such as Analyzing, Evaluating, and Creating, whereas 73.3% of tasks concentrate on LOTS, especially in Understanding and Applying. This disparity implies that fundamental cognitive abilities are being overemphasized, which is preventing children from engaging in critical analysis, evaluation, and innovation. The study emphasizes the need for textbook changes that support students' cognitive growth and are in line with contemporary educational objectives by better integrating HOTS-oriented tasks. Recommendations for curriculum enhancement and instructional strategies are provided to better prepare students for real-world challenges through more complex cognitive engagement.
Exploring the Application of Constructivist Theory in English Language Teaching
Authors: Guo Xinzhe 
Published: December 2024

This paper explores the application of constructivist theory in English language teaching (ELT). This report focuses on three essential methods: situational learning, task-based teaching, and collaborative writing. Swiss psychologist Jean Piaget first introduced constructivist theory and later developed by Soviet psychologist Lev Vygotsky. The active production of knowledge via social interaction and real-world circumstances is emphasized by constructivist theory. The review first explores situational learning, which uses actual settings to improve language acquisition in real-life contexts. Case studies show how well it works to enhance students' communication skills. After that, the topic of task-based education is covered, emphasizing strategies for task design and assessment that are focused on the completion of meaningful activities. According to reviews, collaborative writing is a technique that improves language proficiency through group communication, enhancing vocabulary richness, grammatical accuracy, and critical thinking. The review concludes by summarizing the key findings and discussing challenges in implementation, such as task design and group dynamics. It offers suggestions for future research, particularly in refining assessment techniques and integrating technology into these constructivist approaches.