bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of PTH and corticotomy on implant movement under mechanical force
Authors: Kim Jiyeon , Kim Heon-Young , Kim Won-Ho , Kim Jin-Woo , Kim Minji 
Published: August 2020

Abstract
Background Osseointegrated implants are considered as clinically non-movable. Parathyroid hormone (PTH) is known to play a significant role in the regulation of bone remodeling and in intermittent, low doses, result in osteoanabolic effects. This study aimed to investigate the effects of PTH and corticotomy, both under traction force, on osseointegrated implants. Methods Four implants—two in each hemimandible—were placed in each of the three study mongrels. Each mongrels were designated as control, normal dose PTH (PTH-1), and high dose PTH (PTH-2) groups, with each groups further subdivided into non-surgery and surgery groups. After osseointegration, mechanical force with NiTi closed coil springs (500g) was applied around each implants. Corticotomy was performed around one of four implants in each mongrels. Parathyroid hormone was administered locally on a weekly basis for 20 weeks. Clinical movement of the implants were evaluated with the superimposed 3D- scanned data, bone- microarchitectural and histologic examinations Results Superimposition analysis showed continuous movement of the implants in PTH-1 group. Movement was further justified with lowest bone implant contact (adjusted BIC; 44.77%) in histomorphometric analysis. Upregulation of bone remodeling around the implant was observed in the normal dose PTH group. In the surgery subgroups, the remarkably higher adjusted BIC compared to the non-surgery subgroups indicated increased bone formation around the implant surface. Conclusion The results indicate that the balance of osseointegrated implants in bone remodeling can be shifted via various interventions. Clinical Relevance Upregulated bone remodeling by PTH and corticotomy under continuous mechanical force showed the possible implications on the clinical implant movement.
Sleep Quality and Athletic Performance According to Chronotype
Authors: Lim Seung-Taek , Kim Do-Yoon , Kwon Hyeong-Tae , Lee Eunjae 
Published: August 2020

Abstract
Background: When studying the quality of sleep in relation to athletic performance, the athlete's chronotype and habitual time were considered important factors. We aimed to investigate the sleep quality and athletes’ performance according to chronotype in elite athletes. Methods: Three hundred forty elite athletes (males = 261, females = 79) were recruited for the present study. All participants were screened for chronotype. Measurements of the Korean versions of the Morningness - Eveningness Questionnaire (MEQ-K), Pittsburgh Sleep Quality Index (PSQI), and Wingate Anaerobic Test (WAnT). Results: PSQI global score, PSQI sleep quality, PSQI sleep onset latency, PSQI sleep disturbance, and PSQI daytime dysfunction were significantly difference in among the groups. Mean power, and peak power were significantly difference in among the groups. A negative correlation was found between PSQI score and mean power (W), mean power (W/kg), peak power (W), and peak power (W/kg). Conclusions: This study indicates that related poor sleep quality and late type of chronotype may reduce the athletes’ performance in elite athletes. In additional, significant better sleep quality in early type of chronotype more than late type of chronotype. Moreover, also significant higher athletic performance in early type of chronotype more than late type of chronotype.
Reliability of genomic variants across different next-generation sequencing platforms and bioinformatic processing pipelines
Authors: Gerber Susanne , Weißbach Stephan , Sys Stanislav Jur`Evic , Hewel Charlotte , Todorov Hristo , Schweiger Susann , Winter Jennifer , Pfenninger Markus , Torkamani Ali , Evans Doug , Burger Joachim , Everschor-Sitte Karin , May-Simera Helen 
Published: August 2020

Abstract
Background Next Generation Sequencing (NGS) is the fundament of various studies providing insights into questions from biology and medicine. Nevertheless, integrating data from different experimental backgrounds can introduce strong biases. In order to methodically investigate the magnitude of systematic errors, we performed a cross-sectional observational study on a genomic cohort of 99 subjects each sequenced via (i) Illumina HiSeq X, (ii) Illumina HiSeq and (iii) Complete Genomics. Consequently, we systematically analyzed the heterogeneity between the sequencing cohorts with respect to genomic annotation and common filter criteria like minimum allele frequency (MAF). Results The number of detected variants/variant classes per individual was highly dependent on the sequencing technology. We observed a statistically significant overrepresentation of variants uniquely called by a single platform which indicates potential systematic biases. These variants were enriched in low complexity genomic regions and simple repeats. Furthermore, estimates of allele frequency were highly discrepant for a subset of variants in pairwise comparisons between different sequencing platforms. Applying common filters – such as MAF 5% and HWE- greatly reduced the heterogeneity between cohorts but still left discrepancies of several thousand variants after filtering.Conclusion We provide empirical evidence of systematic heterogeneity in variant calls between alternative experimental and data analysis setups. Our results highlight the potential benefit of reprocessing genomic data with harmonized pipelines when integrating data from different studies.
Mental Health and HIV-related Risky Sexual Behaviors Among Cisgender, Transgender, and Gender Non- Conforming MSM in China
Authors: Duan Zhizhou , Wang Liyin , Guo Menglan , Ding Changmian , Huang Danqin , Yan Hong , Wilson Amanda , Li Shiyue 
Published: August 2020

Abstract
Background
The new recognition of sub-groups among gender minorities (i.e transgender and gender non-conforming) who also identify as men who have sex with men (MSM) play a considerable role in new HIV infections in China. However, while research focuses on the prevalence of MSM, it ignores the diversity of gender minorities within the MSM population. Furthermore, information on the mental health and HIV-related risky sexual behavior also requires consideration to understand the prevalence and new rates of infection both of MSM and within these gender minority sub-groups.
Methods
From September 2017 to December 2017, MSM were recruited in Wuhan, Nanchang, and Changsha cities in China. Participants were asked to fill out a structured self-designed questionnaire to assess depression, perceived social support, resilience, identify concealment, and HIV-related risky sexual behaviors.
Results
A total of 715 MSM completed the structured questionnaire, the number of gender minorities identifying as MSM were 63 and accounted for 8.8% of the population. Compared with the cisgender MSM population, transgender MSM had a significantly lower likelihood of identity concealment (P = 0.016, 95%CI = 0.16, 5.79), were more likely to have one-night stand/occasional partner in the past six months (AOR = 3.90, 95% CI = 1.17–13.03), have sex after drug use (AOR = 2.84, 95%CI = 1.18–6.79), and engage in commercial sexual behavior in the past six months (AOR = 6.09, 95%CI = 1.003–36.94). In terms of gender non-conforming MSM, the differences were not significant for mental health and HIV-related risky sexual behaviors in comparison to the cisgender MSM population.
Conclusions
It is critical to create targeted interventions tailored towards the different gender minority identities among the MSM population. Further research is necessary to understand the relationship between gender identity, mental health, and HIV-related risky sexual behaviors.
Plasmacytoid urothelial carcinoma of renal pelvis with positive zinc finger E–box–binding homeobox 1: a case report
Authors: Takada-Owada Atsuko , Nozawa Yumi , Onozaki Masato , Noda Shuhei , Jamiyan Tsengelumaa , Nakazato Yoshimasa , Tokura Yuumi , Kamai Takao , Ishida Kazuyuki 
Published: August 2020

Abstract
BackgroundThe tumor transformation mechanism of a plasmacytoid urothelial carcinoma remains unexplained. We describe the case of a plasmacytoid urothelial carcinoma of the renal pelvis in which the expression of zinc finger E–box–binding homeobox 1 (ZEB1), a key nuclear transcription factor in an epithelial–mesenchymal transition, is involved in tumor transformation.Case presentationThe patient had a left nephrectomy with the clinical diagnosis of left pelvic renal cancer. The resected specimen showed that the tumor surface comprised a noninvasive papillary urothelial carcinoma with the carcinoma in situ, and the invasive area comprised a plasmacytoid urothelial carcinoma characterized by the presence of single dyscohesive malignant cells that resembled plasma cells in a loose myxoid stroma. The noninvasive urothelial carcinoma was positive for cytokeratin and E–cadherin, and negative for vimentin and ZEB1. In contrast, the invasive plasmacytoid urothelial carcinoma was positive for cytokeratin and also vimentin and ZEB1, and negative for E–cadherin. Additionally, this component was immunoreactive for CD138 and CD38 that are immunohistochemical markers for plasma cells.ConclusionWe suggest that ZEB1 is involved in the plasmacytoid transformation by repressing the E–cadherin in a plasmacytoid urothelial carcinoma.
The association of interpregnancy interval and mode of delivery with outcomes among a cohort of Guatemalan women with a history of prior cesarean
Authors: Harrison Margo , Garces Ana , Figueroa Lester , Westcott Jamie , Hambidge Michael , Krebs Nancy F 
Published: August 2020

Abstract
Design: Our objectives were to analyze how interpregnancy interval (IPI) was associated with delivery mode and how outcomes varied by these characteristics.Methods: This secondary analysis used data from a prospective study conducted in Chimaltenango, Guatemala from January 2017 through April 2020.Results: Of 26,465 Guatemalan women, 3,170 (12.0%) had a history of prior cesarean. 560 (20.1%) women delivered by vaginal birth after cesarean with the remaining 2,233 (79.9%) delivered by repeat cesarean delivery. Repeat cesarean reduced the risk of needing a dilation and curettage compared to vaginal birth after cesarean, but this association did not vary by IPI (AOR 0.01 – 0.03, p < 0.001). Repeat cesarean delivery, as compared to vaginal birth after cesarean, significantly reduced the likelihood a woman breastfeeding within one hour of birth (AOR 0.009 – 0.10, p < 0.001), but IPI was not associated with the outcome. Regarding stillbirth, repeat cesarean birth reduced the likelihood of stillbirth as compared to vaginal birth (AOR 0.2, p = 0.001 – 0.002), but again IPI was not associated with the outcome.Conclusion: Outcomes by mode of delivery among a Guatemalan cohort of women with a history of prior cesarean birth do not vary by IPI.
Health Professionals Perception And Beliefs About Drug- Related Problems on Polymedicated Older Adults- A Focus Group Study
Authors: Plácido Ana Isabel , Herdeiro Maria Teresa , Simões João Lindo , Amaral Odete , Figueiras Adolfo , Roque Fátima 
Published: August 2020

Abstract
Background: Polymedicated older patients are at greater risk of suffering adverse events. For this reason, the detection of both inappropriate polypharmacy and polypharmacy-associated drug-related problems (DRPs) are essential to improve the health and wellbeing of older adults and to reduce healthcare costs. This work aims to explore health professionals’ perceptions and opinions about polypharmacy and the handling of medicines by polymedicated older adults.Methods: Thirteen focus groups with 94 health professionals (20 community pharmacists, 40 general practitioners and 34 nurses) were conducted in primary healthcare centers of the region center of Portugal. Participants were asked to discuss their perceptions and beliefs in relation to DRPs in polymedicated older adults. The sessions were audiotaped. After the transcription and coding of focus group sessions, a thematic analysis was done.Results: The following four main themes emerged f﻿rom the 13 focus group sessions: poor compliance and polypharmacy- a vicious cycle perpetuated by the behaviour of older adults, organization of the healthcare system, communication among health professionals, and strategies to prevent inappropriate polypharmacy.Conclusion: The lack of both an efficient network of information and communication among health professionals makes the detection and/ or prevention of polypharmacy in older adults difficult. The implementation of new models to manage and/or prevent polypharmacy based on health professional perception and beliefs is essential to prevent DRPs and improve compliance among older adults.
District-led Malaria Surveillance and Response as an Effective way to Manage Malaria Upsurges Following the Withdrawal of Indoor Residual Spraying: A Case Study from Nwoya District, Northern Uganda
Authors: Nuwa Anthony , Oola Janet , Obonyo Sam Okot , Feldman Mitra , Karungi Shirah Christine , Kertho Edmound , Odong David Salandini , Kimera Isaac , Magumba Godfrey , Beinomugisha Geofrey , Chitty Alexandra , Tibenderana James Kananura , Opigo Jimmy , Abwaimo Francis 
Published: August 2020

Abstract
BackgroundMalaria remains the number one cause of morbidity and mortality in Uganda. In 2009, an indoor residual spraying (IRS) programme was implemented in 10 Northern districts, resulting in marked reductions in the malaria burden, and a prevalence of just 7 percent. IRS was withdrawn after five years and malaria prevalence among children subsequently increased dramatically from 2014-2016. This upsurge led to an increased focus on training district malaria programme staff on malaria surveillance, epidemic preparedness, and response. An increase in malaria cases was detected again in 2018 in Nwoya district. MethodsIn response to the increase in malaria cases, the District Health Team (DHT) set up multi-sectoral management taskforce and technical rapid response team (RRT). The RRT was responsible for a rapid assessment, mapping exercises and identifying possible causes. The DHT informed all relevant stakeholders of the findings, mobilised resources and developed a comprehensive response plan. Interventions were categorised as immediate (to treat cases and avert deaths), intermediate (to bring down the epidemic) and long-term (to sustain the gains achieved). Health workers from the affected areas were trained on integrated management of malaria, village health team (VHT) members were mentored on malaria epidemic response and their role in the response plan, and health facilities conducted test, treat and track outreach activities. Social behaviour change and communication (SBCC) played a key role, and the RRT monitored and ensured that there were enough malaria commodities at the facilities and for the VHTs.ResultsThe Nwoya DHT was able to detect the 2018 upsurge from the district records, raise awareness, and respond effectively in a timely manner. This district-led response led to a reduction in routinely reported malaria cases from an average of 2,513 cases per week to 1,335 cases between week 43 in 2018 and week 10 in 2019.ConclusionsMalaria upsurges usually have early warning signals and surveillance is a key health intervention to reducing their impact. Malaria surveillance and response, with precisely targeted multipronged activities, when led by local health authorities is an effective, efficient, and sustainable approach to managing such upsurges.
Gut Microbiota: Effect of Pubertal Status
Authors: Yuan Xin , Chen Ruimin , Zhang Ying , Lin Xiangquan , Yang Xiaohong 
Published: August 2020

Abstract
Background: The make-up of gut microbiota at different puberty stages has not been reported. This cross-sectional study analyzed the bio-diversity of gut microbiota at different puberty stages. Methods: The fecal microbiome was determined in 89 Chinese subjects aged 5-15 years. Subjects were grouped as non-pubertal (n=42) or pubertal (n=47) according to Tanner stages. Gut colonization patterns were determined by 16S rDNA microbiome profiling.Results: The subjects were divided into non-pubertal (n=42, male%: 66.7%) or pubertal groups (n=47, male%:44.68); in both groups, Firmicutes, Bacteroidetes and Proteobacteria were the dominant phylum. There was no difference of alpha- and beta-diversity among disparate puberty stages. Non-pubertal subjects had significantly higher members of the genus Turicibacter and lower members of genus Sutterella than pubertal subjects. Of note, the proportion of genus Sutterella increased gradually with the pubertal status and independent of BMI-Z. In the pubertal subjects, the abundance of genus Adlercreutzia, Dorea, Clostridium and Parabacteroides was associated with the level of testosterone.Conclusion: This is the first report of the diversity of gut microbiota at different puberty stages. The various species of gut microbiota changed gradually associated with puberty stages. Differences in gut microflora at different pubertal status may be related to androgen levels.
Predicting Outcomes of Abdominal Surgical Emergencies in the Elderly Population Using a CT Muscle Gauge
Authors: Tee Yu-San , Cheng Chi-Tung , Wu Yu-Tung , Hsu Chih-Po , Kang Shih-Ching , Hsieh Chi-Hsun , Derstine Brian A. , Su Grace L. , Wang Stewart C. , Fu Chih-Yuan , Liao Chien-Hung 
Published: August 2020

Abstract
Background: Frailty has been shown to be an independent negative predictor of surgical outcomes in geriatric patients. Traditional measurements of frailty are impractical in emergency settings, and computed tomography (CT)-measured skeletal muscle mass has been proposed as an alternative. However, the cutoff values of these CT metrics for frailty are still unknown, and their impact on abdominal emergencies in the elderly population is unclear.Study Design: A total of 462 young trauma patients aged 18-40 years were analyzed to establish sex-specific reference cutoff values for the CT-measured muscle index (MI) and muscle gauge (MG) values. The impacts of low MI and MG values were investigated in 1192 elderly patients (aged ≥ 65 years) undergoing abdominal surgery.Results: The sex-specific cutoff values for MI and MG were determined by adopting European Working Group on Sarcopenia in Older People (EWGSOP) guidelines. The correlation between MG and ageing was significantly stronger than that between MI and ageing. With regard to the MG, the L4 psoas muscle gauge (L4 PMG) was further investigated in an elderly cohort owing to its high predictive value and ease of use in the clinical setting. A low L4 PMG value was an independent risk factor for overall complications and mortality in elderly patients with abdominal emergencies.Conclusion: The current study was the largest study investigating the correlations between MG values and ageing in the Asian population. Frailty, as indicated by a low L4 PMG value, may help surgeons during preoperative decision making regarding geriatric patients with abdominal emergencies.
Insoluble proteins catch heterologous soluble proteins into inclusion bodies by intermolecular interaction of aggregating peptides
Authors: Carratalá Jose Vicente , Cisneros Andrés , Hellman Elijah , Villaverde Antonio , Ferrer-Miralles Neus 
Published: August 2020

Abstract
Background
Protein aggregation is a biological event observed in expression systems in which the recombinant protein is produced under stressful conditions surpassing the homeostasis of the protein quality control system. In addition, protein aggregation is also related to conformational diseases in animals as transmissible prion diseases or non-transmissible neurodegenerative diseases including Alzheimer, Parkinson's disease, amyloidosis and multiple system atrophy among others. At the molecular level, the presence of aggregating-prone domains in protein molecules act as seeding igniters to induce the accumulation of protein molecules in protease-resistant clusters by intermolecular interactions.
Results
In this work we have studied the aggregating-prone performance of a small peptide (L6K2) with additional antimicrobial activity and we have elucidated the relevance of the accompanying scaffold protein to enhance the aggregating profile of the fusion protein. Furthermore, we demonstrated that the fusion of L6K2 to highly soluble recombinant proteins directs the protein to inclusion bodies (IB) in E. coli through stereospecific interactions in the presence of an insoluble protein displaying the same aggregating-prone peptide (APP).
Conclusions
These data suggest that the molecular bases of protein aggregation are related to the net balance of protein aggregation potential and not only to the presence of aggregation-prone stretches. This is then presented as a generic platform to generate hybrid protein aggregates in microbial cell factories for biopharmaceutical and biotechnological applications.
Laparoscopically resected perivascular epithelioid cell tumor of the ascending colon demonstrating submucosal tumor morphology: a case report
Authors: Nagai Yuzo , Kiyomatsu Tomomichi , Kume Ayako , Kataoka Atsuko , Gohda Yoshimasa , Otani Kensuke , Deguchi Katsuya 
Published: August 2020

Abstract
Background: The differential diagnosis for a colonic submucosal tumor (SMT) can include many types of cancer. We report a case of a perivascular epithelioid cell tumor (PEComa), a rare mesenchymal neoplasm that demonstrates SMT morphology, in the ascending colon.Case presentation: A mass was incidentally detected by computed tomography (CT) in a 53-year-old man. Colonoscopy revealed an SMT with central ulceration in the ascending colon, and 18F-fluoro-2-deoxy-D-glucose positron emission tomography CT demonstrated a high maximum standardized uptake value of 7.33. Preoperative diagnosis was inconclusive. Given the malignant potential of the tumor, we performed a laparoscopic right hemicolectomy with D3 lymph node dissection. The tumor was 5.5 cm × 4.1 cm and located in the submucosa and muscular propria. There was no lymph node metastasis (0/46). Based on the positive immunohistochemical stainings of both melanocytic (HMB45) and muscle (desmin) markers, we diagnosed the tumor as a PEComa. In the 17 months since surgical resection, the patient has not experienced recurrence, but careful observation should continue because of the malignant potential of the tumor.Conclusions: Although rare, a PEComa should be considered in the differential diagnosis of colonic SMT. Immunohistochemical staining of melanocytic and muscle markers is useful for the diagnosis.