bluuebunny/crossref_metadata_2025_split huggingface dataset:
Estimation of High-Speed Liquid-Jet Velocity Using a Pyro Jet Injector
Authors: Takagaki Naohisa , Kitaguchi Toru , Iwayama Masashi , Shinoda Atsushi , Kumamaru Hiroshige , Honda Itsuro 
Published: December 2019

AbstractThe high-speed liquid-jet velocity achieved using an injector strongly depends on the piston motion, physical property of the liquid, and container shape of the injector. Herein, we investigate the liquid ejection mechanism and a technique for estimating the ejection velocity of a high-speed liquid jet using a pyro jet injector (PJI). We apply a two-dimensional numerical simulation with an axisymmetric approximation using the commercial software ANSYS/FLUENT. To gather the input data applied during the numerical simulation, the piston motion is captured with a high-speed CMOS camera, and the velocity of the piston is measured using motion tracking software. To reproduce the piston motion during the numerical simulation, the boundary-fitted coordinates and a moving boundary method are employed. In addition, we propose a fluid dynamic model (FDM) for estimating the high-speed liquid-jet ejection velocity based on the piston velocity. Using the FDM, we consider the liquid density variation but neglect the effects of the liquid viscosity on the liquid ejection. Our results indicate that the liquid-jet ejection velocity estimated by the FDM corresponds to that predicted by ANSYS/FLUENT for several different ignition-powder weights. This clearly shows that a high-speed liquid-jet ejection velocity can be estimated using the presented FDM when considering the variation in liquid density but neglecting the liquid viscosity. In addition, some characteristics of the presented PJI are observed, namely, (1) a very rapid piston displacement within 0.1 ms after a powder explosion, (2) piston vibration only when a large amount of powder is used, and (3) a pulse jet flow with a temporal pulse width of 0.1 ms.
Nanostructured silver dendrites for photon-induced Cysteine dimerization
Authors: Chang Chia‐Yu , Chen Yu‐Mei , Huang Yu‐Bin , Lai Chin-Hung , Jeng U-Ser , Lai Ying-Huang 
Published: December 2019

AbstractUnder a controlled adsorption environment, L-cysteine molecules can be chemically adsorbed to the dendritic silver (Ag-D) surface by electrochemical methods with different functional groups. It is verified by surface-enhanced Raman spectroscopy that under alkaline conditions (pH = 13.50), the two functional groups of thiol and acid are simultaneously adsorbed on the surface of Ag-D, while NH2 is far from the surface; under acidic conditions (pH = 1.67), adsorption behavior suggests that both NH3+ and COO− are oriented toward the Ag-D surface, and that SH is far from the surface. The structure of L-cysteine adsorption under acidic conditions can be further verified by the addition of an L-cysteine molecule through light-induced coupling reaction to form cystine. Finally, in-situ two-dimensional Raman scattering spectroscopy confirmed the feasibility and uniformity of the coupling reaction.
Diagnostic accuracy of computed tomography angiography for the exclusion of coronary artery disease in candidates for transcatheter aortic valve implantation
Authors: Strong Christopher , Ferreira António , Teles Rui Campante , Mendes Gustavo , Abecasis João , Cardoso Gonçalo , Guerreiro Sara , Freitas Pedro , Santos Ana Coutinho , Saraiva Carla , Brito João , Raposo Luís , Gonçalves Pedro de Araújo , Gabriel Henrique Mesquita , Almeida Manuel de Sousa , Mendes Miguel 
Published: December 2019

AbstractCoronary CT angiography (CTA) is currently considered a reliable method to exclude obstructive coronary artery disease (CAD) before valvular heart surgery in patients with low pretest probability. However, its role in excluding obstructive CAD before transcatheter aortic valve implantation (TAVI) is less well established. Single-center retrospective study where patients with severe symptomatic aortic stenosis underwent both CTA and invasive coronary angiography (ICA) as part of TAVI planning. CTA exams were conducted on a 64-slice dual source scanner, with a median interval of 45 days to ICA (IQR 25–75 [13–82]). In both tests, obstructive CAD was defined as a ≥50% stenosis in an epicardial vessel ≥2 mm diameter. Per-patient, per-vessel and per-proximal segment analyses were conducted, excluding and including non-evaluable segments. The study included 200 patients (120 women, mean age 83 ± 6 years). The prevalence of obstructive CAD on ICA was 35.5% (n = 71). On a per-patient analysis (assuming non-evaluable segments as stenotic), CTA showed sensitivity of 100% (95% CI, 95–100%), specificity of 42% (95% CI, 33–51%), and positive and negative predictive values of 48% (95% CI, 44–51%) and 100% (95% CI, 92–100%), respectively. CTA was able to exclude obstructive CAD in 54 patients (27%), in whom ICA could have been safely withheld. Despite the high rate of inconclusive tests, pre-procedural CTA is able to safely exclude obstructive CAD in a significant proportion of patients undergoing TAVI, possibly avoiding the need for ICA in roughly one quarter of the cases.
Contribution of protein synthesis depression to poly-β-hydroxybutyrate accumulation in Synechocystis sp. PCC 6803 under nutrient-starved conditions
Authors: Hirai Kazuho , Nojo Miki , Sato Yosuke , Tsuzuki Mikio , Sato Norihiro 
Published: December 2019

AbstractPoly-β-hydroxybutyrate (PHB) in cyanobacteria, which accumulates as energy and carbon sources through the action of photosynthesis, is expected to substitute for petroleum-based plastics. This study first demonstrated that PHB accumulation was induced, with the appearance of lipid droplets, in sulfur (S)-starved cells of a cyanobacterium, Synechocystis sp. PCC 6803, however, to a lower level than in nitrogen (N)- or phosphorus (P)-starved cells. Concomitantly found was repression of the accumulation of total cellular proteins in the S-starved cells to a similar level to that in N-starved cells, and a severer level than in P-starved cells. Intriguingly, PHB accumulation was induced in Synechocystis even under nutrient-replete conditions, upon repression of the accumulation of total cellular proteins through treatment of the wild type cells with a protein synthesis inhibitor, chloramphenicol, or through disruption of the argD gene for Arg synthesis. Meanwhile, the expression of the genes for PHB synthesis was hardly induced in S-starved cells, in contrast to their definite up-regulation in N- or P-starved cells. It therefore seemed that PHB accumulation in S-starved cells is achieved through severe repression of protein synthesis, but is smaller than in N- or P-starved cells, owing to little induction of the expression of PHB synthesis genes.
Simulation of Glacial Avalanche Hazards in Shyok Basin of Upper Indus
Authors: Gilany Naseem , Iqbal Javed 
Published: December 2019

AbstractGlacial avalanche hazards can threaten lives and damage infrastructures in high altitude mountainous regions. In April 2012 a gigantic ice plus rock avalanche destroyed military camp at Gyari and killed 139 persons. Antecedent, the objectives of this study are to simulate and model Gyari camp glacial avalanche with reference to its extent, height, momentum, velocity and pressure and, to simulate and model the other potential glacial avalanche prone areas in Shyok basin. To simulate the Gyari camp glacial hazard and other potential glacial avalanche hazards, an empirical process based Glacier Avalanche Model; Rapid Access Mass Movement Simulation (RAMMS) is used. The RAMMS model encompasses the variables like avalanche release height and release area for the conduct of simulation. The model output of Gyari glacial avalanche hazard resulted from a max pressure of 2500 KPa, max velocity of 90 m/s, and the max flow height of 40 m, while the resulted output debris volume calculated was 4.3145 million m3. The calibrated agreement was found in extent and height of actual debris in comparison with RAMMS simulated output. The potential hazardous glacial avalanche prone areas of Shyok basin were simulated by RAMMS model after the model being calibrated to the actual incident of Gyari. The study has resulted in identifying the Siachen glacier conflict zone being more prone to avalanche hazards because of host factors in general and the anthropogenic factor in particular.
Database of adverse events associated with drugs and drug combinations
Authors: Poleksic Aleksandar , Xie Lei 
Published: December 2019

AbstractDue to the aging world population and increasing trend in clinical practice to treat patients with multiple drugs, adverse events (AEs) are becoming a major challenge in drug discovery and public health. In particular, identifying AEs caused by drug combinations remains a challenging task. Clinical trials typically focus on individual drugs rather than drug combinations and animal models are unreliable. An added difficulty is the combinatorial explosion in the number of possible combinations that can be made using the increasingly large set of FDA approved chemicals. We present a statistical and computational technique for identifying AEs caused by two-drug combinations. Taking advantage of the large and increasing data deposited in FDA’s postmarketing reports, we demonstrate that the task of predicting AEs for 2-drug combinations is amenable to the Likelihood Ratio Test (LRT). Our pAERS database constructed with LRT contains almost 77 thousand associations between pairs of drugs and corresponding AEs caused solely by drug-drug interactions (DDIs). The DDIs stored in pAERS complement the existing data sets. Due to our stringent statistical test, we expect many of the associations in pAERS to be unrecorded or poorly documented in the literature.
Genome-Wide SNP discovery and genomic characterization in avocado (Persea americana Mill.)
Authors: Talavera Alicia , Soorni Aboozar , Bombarely Aureliano , Matas Antonio J. , Hormaza Jose I. 
Published: December 2019

AbstractModern crop breeding is based on the use of genetically and phenotypically diverse plant material and, consequently, a proper understanding of population structure and genetic diversity is essential for the effective development of breeding programs. An example is avocado, a woody perennial fruit crop native to Mesoamerica with an increasing popularity worldwide. Despite its commercial success, there are important gaps in the molecular tools available to support on-going avocado breeding programs. In order to fill this gap, in this study, an avocado ‘Hass’ draft assembly was developed and used as reference to study 71 avocado accessions which represent the three traditionally recognized avocado horticultural races or subspecies (Mexican, Guatemalan and West Indian). An average of 5.72 M reads per individual and a total of 7,108 single nucleotide polymorphism (SNP) markers were produced for the 71 accessions analyzed. These molecular markers were used in a study of genetic diversity and population structure. The results broadly separate the accessions studied according to their botanical race in four main groups: Mexican, Guatemalan, West Indian and an additional group of Guatemalan × Mexican hybrids. The high number of SNP markers developed in this study will be a useful genomic resource for the avocado community.
Dynamic, Simultaneous Concentration Mapping of Multiple MRI Contrast Agents with Dual Contrast - Magnetic Resonance Fingerprinting
Authors: Anderson Christian E. , Johansen Mette , Erokwu Bernadette O. , Hu He , Gu Yuning , Zhang Yifan , Kavran Michael , Vincent Jason , Drumm Mitchell L. , Griswold Mark A. , Steinmetz Nicole F. , Li Ming , Clark Heather , Darrah Rebecca J. , Yu Xin , Brady-Kalnay Susann M. , Flask Chris A. 
Published: December 2019

AbstractSynchronous assessment of multiple MRI contrast agents in a single scanning session would provide a new “multi-color” imaging capability similar to fluorescence imaging but with high spatiotemporal resolution and unlimited imaging depth. This multi-agent MRI technology would enable a whole new class of basic science and clinical MRI experiments that simultaneously explore multiple physiologic/molecular events in vivo. Unfortunately, conventional MRI acquisition techniques are only capable of detecting and quantifying one paramagnetic MRI contrast agent at a time. Herein, the Dual Contrast – Magnetic Resonance Fingerprinting (DC-MRF) methodology was extended for in vivo application and evaluated by simultaneously and dynamically mapping the intra-tumoral concentration of two MRI contrast agents (Gd-BOPTA and Dy-DOTA-azide) in a mouse glioma model. Co-registered gadolinium and dysprosium concentration maps were generated with sub-millimeter spatial resolution and acquired dynamically with just over 2-minute temporal resolution. Mean tumor Gd and Dy concentration measurements from both single agent and dual agent DC-MRF studies demonstrated significant correlations with ex vivo mass spectrometry elemental analyses. This initial in vivo study demonstrates the potential for DC-MRF to provide a useful dual-agent MRI platform.
The Discovery of Twenty-Eight New Encapsulin Sequences, Including Three in Anammox Bacteria
Authors: Tracey John C. , Coronado Maricela , Giessen Tobias W. , Lau Maggie C. Y. , Silver Pamela A. , Ward Bess B. 
Published: December 2019

AbstractMany prokaryotes encode protein-based encapsulin nanocompartments, including anaerobic ammonium oxidizing (anammox) bacteria. This study expands the list of known anammox encapsulin systems from freshwater species to include the marine genus Scalindua. Two novel systems, identified in “Candidatus Scalindua rubra” and “Candidatus Scalindua sp. SCAELEC01 167” possess different architectures than previously studied freshwater anammox encapsulins. Characterization of the S. rubra encapsulin confirms that it can self-assemble to form compartments when heterologously expressed in Escherichia coli. BLASTp and HMMER searches of additional genomes and metagenomes spanning a range of environments returned 26 additional novel encapsulins, including a freshwater anammox encapsulin identified in “Candidatus Brocadia caroliniensis”. Phylogenetic analysis comparing these 28 new encapsulin sequences and cargo to that of their closest known relatives shows that encapsulins cluster by cargo protein type and therefore likely evolved together. Lastly, prokaryotic encapsulins may be more common and diverse than previously thought. Through searching a small sample size of all public metagenomes and genomes, many new encapsulin systems were unearthed by this study. This suggests that many additional encapsulins likely remain to be discovered.
Comorbidity status in hospitalized elderly in Japan: Analysis from National Database of Health Insurance Claims and Specific Health Checkups
Authors: Nojiri Shuko , Itoh Hiroaki , Kasai Takatoshi , Fujibayashi Kazutoshi , Saito Tomoyuki , Hiratsuka Yoshimune , Okuzawa Atsushi , Naito Toshio , Yokoyama Kazuhito , Daida Hiroyuki 
Published: December 2019

AbstractThe detailed comorbidity status of hospitalized elderly patients throughout Japan has remained largely unknown; therefore, our goal was to rigorously explore this situation and its implications as of the 2015 fiscal year (from April 2015 to March 2016). This study was based on a health insurance claims database, covering all insured policy holders in Japan aged ≥60 years (male: n = 2,135,049, female: 1,969,019) as of the 2015 fiscal year. Comorbidity status was identified by applying principal factor analysis to the database. The factors identified in male patients were [1] myocardial infarction, hypertension, dyslipidemia, and diabetes mellitus; [2] congestive heart failure (CHF), cardiac arrhythmia, and renal failure; [3] Parkinson’s disease, dementia, cerebrovascular disease, and pneumonia; [4] cancer and digestive disorders; and [5] rheumatoid arthritis and hip fracture. However, in female patients, the results obtained for the quaternary and quinary factors were the opposite of those obtained in male patients. In superelderly patients, dementia, cerebrovascular disease, and pneumonia appeared as the tertiary factor, and hip fracture and osteoporosis appeared as the quaternary factor. The comorbidities in the elderly patients suggest the importance of coronary heart disease and its related metabolic disorders; in superelderly patients, fracture and osteoporosis appeared as factors, in addition to dementia and pneumonia.
Electronic structure of the PrNiBi half-Heusler system based on the σGGA + U method
Authors: Mikaeilzadeh L. , Tavana A. , Khoeini F. 
Published: December 2019

AbstractIn this works, we study the electronic structure and magnetic properties of the Pr-Ni-Bi half-Heusler systems based on density functional theory. We use the σ GGA + U scheme to consider the effects of on-site electron-electron interactions. Results show that in contrast to the rough estimation of the total magnetic moment of the unit cell, based on the Slater-Pauling behavior in the half-Heusler systems, this system has an antiferromagnetic ground state because of the localized Pr-f electrons. By increasing the magnitude of the effective U parameter, band-inversion occurs in the band structure of this system, which shows the possibility of topological state occurrence.
The Prognostic Value of Circulating Tumor Cells in Asian Neuroendocrine Tumors
Authors: Hsieh Jason Chia-Hsun , Chen Guan-Yu , Jhou David Da-Wei , Chou Wen-Chi , Yeh Chun-Nan , Hwang Tsann-Long , Lin Hung-Chi , Chu Hui-Chun , Wang Hung-Ming , Yen Tzu-Chen , Chen Jen-Shi , Wu Min-Hsien 
Published: December 2019

AbstractCirculating tumor cells (CTC) play important roles in various cancers; however, few studies have assessed their clinical utility in neuroendocrine tumors. This study aimed to prospectively evaluate the prognostic value of CTC counts in Asian patients with neuroendocrine tumors before and during anti-cancer therapy. Patients who were diagnosed with unresectable histological neuroendocrine tumors between September 2011 and September 2017 were enrolled. CTC testing was performed before and during anti-cancer therapy using a negative selection protocol. Chromogranin A levels were also assessed. Univariate and multivariate Cox’s proportional hazard model with forward LR model was performed to investigate the impact of independent factors on overall survival and progression-free survival. Kaplan–Meier method with log-rank tests were used to determine the difference among different clinicopathological signatures and CTC cutoff. The baseline CTC detection rate was 94.3% (33/35). CTC counts were associated with cancer stages (I-III vs. IV, P = 0.015), liver metastasis (P = 0.026), and neuroendocrine tumor grading (P = 0.03). The median progression-free survival and overall survivals were 12.3 and 30.4 months, respectively. In multivariate Cox regression model, neuroendocrine tumors grading and baseline CTC counts were both independent prognostic factors for progression-free survival (PFS, P = 0.005 and 0.015, respectively) and overall survival (OS, P = 0.018 and 0.023, respectively). In Kaplan-Meier analysis, lower baseline chromogranin A levels were associated with longer PFS (P = 0.024). Baseline CTC counts are associated with the clinicopathologic features of neuroendocrine tumors and are an independent prognostic factor for this malignancy.
Force spectroscopy-based simultaneous topographical and mechanical characterization to study polymer-to-polymer interactions in coated alginate microspheres
Authors: Virumbrales-Muñoz Maria , Santos-Vizcaino Edorta , Paz Laura , Gallardo-Moreno Amparo Maria , Orive Gorka , Hernandez Rosa Maria , Doblaré Manuel , Gonzalez-Martin Maria Luisa , Fernández Luis Jose , Pedraz Jose Luis , Ochoa Ignacio 
Published: December 2019

AbstractCell-laden hydrogel microspheres have shown encouraging outcomes in the fields of drug delivery, tissue engineering or regenerative medicine. Beyond the classical single coating with polycations, many other different coating designs have been reported with the aim of improving mechanical properties and in vivo performance of the microspheres. Among the most common strategies are the inclusion of additional polycation coatings and the covalent bonding of the semi-permeable membranes with biocompatible crosslinkers such as genipin. However, it remains challenging to characterize the effects of the interactions between the polycations and the hydrogel microspheres over time in vitro. Here we use a force spectroscopy-based simultaneous topographical and mechanical characterization to study polymer-to-polymer interactions in alginate microspheres with different coating designs, maintaining the hydrogels in liquid. In addition to classical topography parameters, we explored, for the first time, the evolution of peak/valley features along the z axis via thresholding analysis and the cross-correlation between topography and stiffness profiles with resolution down to tens of nanometers. Thus, we demonstrated the importance of genipin crosslinking to avoid membrane detachment in alginate microspheres with double polycation coatings. Overall, this methodology could improve hydrogel design rationale and expedite in vitro characterization, therefore facilitating clinical translation of hydrogel-based technologies.
Seizure localization using pre ictal phase-amplitude coupling in intracranial electroencephalography
Authors: Cámpora Nuria E. , Mininni Camilo J. , Kochen Silvia , Lew Sergio E. 
Published: December 2019

AbstractUnderstanding changes in brain rhythms provides useful information to predict the onset of a seizure and to localize its onset zone in epileptic patients. Brain rhythms dynamics in general, and phase-amplitude coupling in particular, are known to be drastically altered during epileptic seizures. However, the neural processes that take place before a seizure are not well understood. We analysed the phase-amplitude coupling dynamics of stereoelectroencephalography recordings (30 seizures, 5 patients) before and after seizure onset. Electrodes near the seizure onset zone showed higher phase-amplitude coupling. Immediately before the beginning of the seizure, phase-amplitude coupling dropped to values similar to the observed in electrodes far from the seizure onset zone. Thus, our results bring accurate information to detect epileptic events during pre-ictal periods and to delimit the zone of seizure onset in patients undergoing epilepsy surgery.