bluuebunny/crossref_metadata_2025_split huggingface dataset:
De Novo Transcriptome Sequencing of Serangium japonicum (Coleoptera: Coccinellidae) and Application of Two Assembled Unigenes
Authors: Hu Ya Hui , Liu Yong , Wei Lin , Chen Hao Tao 
Published: November 2019

Abstract
The ladybird beetle Serangium japonicum is an important predator of whiteflies. Investigations of the molecular mechanisms of this predatory beetle have been hindered by the scarcity of gene sequence data. To obtain gene sequences for the ladybird beetle and determine differences in gene expression between the summer and winter seasons, paired-end sequencing was performed. Real-time PCR was used to validate differences in Krueppel homolog 1 gene (Kr-h1) mRNA expression in summer vs. winter samples. To determined the diversity of the population, annotated cytochrome c oxidase subunit I gene (COX1) gene fragments were amplified from several ladybird beetle populations. The analysis yielded 191,246 assembled unigenes, 127,016 of which (66.4%) were annotated. These functional annotations of gene sequences are currently available from the National Center for Biotechnology Information (NCBI), and will provide a basis for studying the molecular mechanisms underlying the biological characteristics of S. japonicum. We found a change in expression of ribosome-associated genes across seasons, and postulate that this change is because of seasonal variation in temperature and photoperiod. The differential expression of Kr-h1 suggests that S. japonicum can successfully overwinter because the adults enter diapause. To explain the effects of season on Kr-h1 gene expression, we hypothesize a model in which that a short photoperiod affects the density of Ca2+, the subsequent activity of methyl farnesoate epoxidase and the synthesis of JH, and in turn Kr-h1 gene expression. COX1 annotation was concordant with the morphological ID. The same COX1 sequence was found in the samples from several provinces in China. Therefore, the COX1 sequence is worth further study to distinguish beetle species and populations.
Clay Mineral Assemblages in Recent Thermal Anomalies of Southern Kamchatka
Authors: Sergeeva A.V. , Denisov D.K. , Nazarova M.A. 
Published: November 2019

Abstract
—We consider the factors determining the mineral composition of hydrothermal clays in recent thermal anomalies of the Pauzhetka–Kambal’nyi–Koshelevskii region and Ebeko (Paramushir Island) and Mutnovskii Volcanoes. The composition of mineral assemblages is influenced by a number of interdependent factors: the type of discharge (steam–gas jets, steaming ground, and mud water pools), temperature, pH, and Eh of a thermal solution. Mineral assemblages including sulfur, opal, α-quartz, and alunite group minerals form on the steaming ground of the craters of active volcanoes (Ebeko and Mutnovskii). Mud water pools of the craters contain mainly sulfur, opal, and α-quartz. An assemblage of kaolinite, opal, alunite group minerals (in small amounts), and goethite forms in mud water pools with pH = 2–3 in the thermal fields of the Pauzhetka–Kambal’nyi–Koshelevskii region. An assemblage of newly formed kaolinite, smectite, pyrite, marcasite, and, sometimes, opal is specific to mud water pools with pH &gt; 3.5–4.0 At pH &gt; 5, smectite, pyrite, and opal are produced. In the thermal fields of the Pauzhetka–Kambal’nyi–Koshelevskii region, the walls of steam–gas jets that are often flooded with water and dry out are formed by kaolinite, smectite, opal, α-quartz, pyrite, marcasite, and goethite. The walls of steam–gas jets that are not flooded with water for a long time consist of kaolinite, opal, α-quartz, smectite (traces), alunite group minerals, pyrite, and marcasite in close amounts. The steaming ground of the thermal fields of the Pauzhetka–Kambal’nyi–Koshelevskii region is composed of kaolinite, alunite group minerals, opal, and goethite or an assemblage of kaolinite, smectite, opal, pyrite, and marcasite.
Along‐Arc Heterogeneity in Local Seismicity across the Lesser Antilles Subduction Zone from a Dense Ocean‐Bottom Seismometer Network
Authors: Bie Lidong , Rietbrock Andreas , Hicks Stephen , Allen Robert , Blundy Jon , Clouard Valerie , Collier Jenny , Davidson Jon , Garth Thomas , Goes Saskia , Harmon Nick , Henstock Tim , van Hunen Jeroen , Kendall Mike , Krüger Frank , Lynch Lloyd , Macpherson Colin , Robertson Richard , Rychert Kate , Tait Stephen , Wilkinson Jamie , Wilson Marjorie 
Published: November 2019

AbstractThe Lesser Antilles arc is only one of two subduction zones where slow‐spreading Atlantic lithosphere is consumed. Slow‐spreading may result in the Atlantic lithosphere being more pervasively and heterogeneously hydrated than fast‐spreading Pacific lithosphere, thus affecting the flux of fluids into the deep mantle. Understanding the distribution of seismicity can help unravel the effect of fluids on geodynamic and seismogenic processes. However, a detailed view of local seismicity across the whole Lesser Antilles subduction zone is lacking. Using a temporary ocean‐bottom seismic network we invert for hypocenters and 1D velocity model. A systematic search yields a 27 km thick crust, reflecting average arc and back‐arc structures. We find abundant intraslab seismicity beneath Martinique and Dominica, which may relate to the subducted Marathon and/or Mercurius Fracture Zones. Pervasive seismicity in the cold mantle wedge corner and thrust seismicity deep on the subducting plate interface suggest an unusually wide megathrust seismogenic zone reaching ∼65  km depth. Our results provide an excellent framework for future understanding of regional seismic hazard in eastern Caribbean and the volatile cycling beneath the Lesser Antilles arc.
The Oklahoma Geological Survey Statewide Seismic Network
Authors: Walter Jacob I. , Ogwari Paul , Thiel Andrew , Ferrer Fernando , Woelfel Isaac , Chang Jefferson C. , Darold Amberlee P. , Holland Austin A. 
Published: November 2019

AbstractThe Oklahoma Geological Survey (OGS) monitors seismicity throughout the state of Oklahoma utilizing permanent and temporary seismometers installed by OGS and other agencies, while producing a real-time earthquake catalog. The OGS seismic network was recently added to the Advanced National Seismic System (ANSS) as a self-supporting regional seismic network, and earthquake locations and magnitudes are automatically reported through U.S. Geological Survey and are part of the ANSS Comprehensive Earthquake Catalog. In Oklahoma, before 2009, background seismicity rates were about 2 M 3.0+ earthquakes per year, which increased to 579 and 903 M 3.0+ earthquakes in 2014 and 2015, respectively. After seismicity peaked, the rate fell to 624, 304, and 194 M 3.0+ earthquakes in 2016, 2017, and 2018, respectively. The catalog is complete down to M 2.2 from mid-2014 to present, despite the significant workload for a primarily state-funded regional network. That astonishing uptick in seismicity has been largely attributed to wastewater injection practices. The OGS provides the Oklahoma Corporation Commission, the agency responsible for regulating oil and gas activities within the state, with technical guidance and earthquake products that inform their “traffic-light” mitigation protocol and other mitigating actions. We have initiated a citizen-scientist-driven, educational seismometer program by installing Raspberry Shake geophones throughout the state at local schools, museums, libraries, and state parks. The seismic hazard of the state portends a continued need for expansion and densification of seismic monitoring throughout Oklahoma.
Pengaruh Pembelajaran Kasti Modifikaasi Terhadap Nilai Kerjasama pada Siswa Sekolah Dasar
Authors: Jaenudin Muhamad Ade , Suherman Adang , Komarudin Komarudin 
Published: November 2019

Penelitian ini bermula dari permasalahan permainan tradisional yang sekarang kurang mendapat tempat sebagai olahraga tradisional dalam kancah pendidikan jasmani. Permainan ini memiliki unsur mendidik kerjasama yang baik, tetapi ada kelemahan dalam pelaksanaannya. Untuk mengurangi kelemahan tersebut dilakukan penelitian permainan kasti modifikasi dan dilihat pengaruhnya terhadap kemampuan kerjasmana siswa sekolah dasar (kalas rendah). Sampel dalam penelitian ini adalah 25 siswa kelas satu sampai tiga yang dipilih secara acak dari populasi. Siswa diberikan perlakukan kasti modifikasi selama 16 kali pertemuan. Dilakukan pre test dan post test untuk mengetahui hasil penelitian yang dilakukan. Tes kerjasama dipergunakan lembar observasi yang dilakukan oleh dua Orang judge. Hasil tes kerjasama setelah mendapatkan perlakukan dan sebelum mendapatkan perlakukan ternyata mengalami perbedaan yang signigikan 0.000 dengan uji T. Rata-rata sebelum dilakukan perlakukan adalah 37,6 dan setelah perlakukan 45,2.Kata kunci: Pendidikan jasmani, Kasti modifikasi, Kerjasama, Siswa sekolah dasar
Effectiveness of a combination of anti-pigmentary products in facial post-inflammatory hyperpigmentation
Authors: Nayak Chitra S. , Ansari Saba M. M. , Salve Vrushali , Patil Shweta 
Published: November 2019

<p class="abstract"><strong>Background:</strong> Post-inflammatory hyperpigmentation (PIH), also has psychosocial implications. Hydroquinone containing preparations though effective, have unacceptable side effects. Molecules like kojic acid, arbutin, vitamins C and E, niacinamide and many others have depigmenting effect, singly or in combination. The objective of this study was to assess the effectiveness and tolerability of a cream containing a combination of niacinamide-4.0%, glycolic acid-2.0%, vitamin E acetate-0.1%, kojic acid dipalmitate-2.0%, soy isoflavones-0.5%, arbutin-2.0%, pterowhite-0.12%, licorice-40% CA-0.12%, ascorbyl glucoside-0.1% (Melaglow-Rich, Abbott Healthcare Pvt. Ltd.) in treatment of PIH. The specific objectives were to evaluate the changes in pigmentation, safety of the cream and patient opinion regarding the cream after 90 days of use.</p><p class="abstract"><strong>Methods:</strong> After IEC approval, adult males and non-pregnant, non-lactating females aged upto 50 years with PIH were included. Those with endocrinopathies who received systemic steroid therapy in the preceding month were excluded. 114 participants who were enrolled in this open-label, non-comparative study, applied study medication (b.i.d) and sunscreen (SPF-30; q.d). Effectiveness was assessed by PIH severity scale, Mexameter assessment, Patients’ and Physicians’ Global Assessment Scale and clinical photographs. Adverse events were documented.<strong></strong></p><p class="abstract"><strong>Results:</strong> Mean PIH score and mean mexameter reading for melanin and erythema in the hyperpigmented and non-hyperpigmented skin reduced by day 90 (p=0.0009, p&lt;0.0001, p&lt;0.001). Significant reduction in physicians’ (p=0.004) and patients’ (p=0.006) global assessment score was evident by day 90. Itching (n=1), burning (n=3) and stinging (n=3) were noted.</p><p class="abstract"><strong>Conclusions:</strong> Melaglow rich cream was found to be effective and well-tolerated in the treatment of PIH.</p>
Cousinia gigantosphaera Rech, f. (Asteraceae-Compositae); Türkiye’nin Güneydoğu Anadolu Bölgesi’nden Yeni Bir Bitki Türü Kaydı
Authors: FİDAN Mehmet , PINAR Süleyman Mesut , EROĞLU Hüseyin 
Published: November 2019

Türkiye’nin Güneydoğu Anadolu Bölgesi’nde yer alan
Şırnak ilinde 2018-2019 yılları arasında Tarım ve Orman Bakanlığı, Doğa Koruma
ve Milli Parklar Genel Müdürlüğü kontrolünde “Şırnak İlinin Karasal ve İç Su
Ekosistemleri Biyolojik Çeşitlilik ve Envanter İzleme Projesi” yapılmıştır. Bu
proje kapsamında yapılan arazi çalışmalarında toplanan Cousinia cinsine ait bazı örneklerin Türkiye’de bulunan
taksonlardan farklılık gösterdiği belirlenmiştir. Bu örneklerin detaylı ve
karşılaştırmalı teşhisi ile Cousinia
gigantosphaera Rech.f olduğu anlaşılmış ve
Türkiye’den (Şırnak, Beytüşşebap) ilk defa kayıt altına alınmıştır.
Opioid Use Fueling HIV Transmission in an Urban Setting: An Outbreak of HIV Infection Among People Who Inject Drugs—Massachusetts, 2015–2018
Authors: Alpren Charles , Dawson Erica L. , John Betsey , Cranston Kevin , Panneer Nivedha , Fukuda H. Dawn , Roosevelt Kathleen , Klevens R. Monina , Bryant Janice , Peters Philip J. , Lyss Sheryl B. , Switzer William M. , Burrage Amanda , Murray Ashley , Agnew-Brune Christine , Stiles Tracy , McClung Paul , Campbell Ellsworth M. , Breen Courtney , Randall Liisa M. , Dasgupta Sharoda , Onofrey Shauna , Bixler Danae , Hampton Kischa , Jaeger Jenifer Leaf , Hsu Katherine K. , Adih William , Callis Barry , Goldman Linda R. , Danner Susie P. , Jia Hongwei , Tumpney Matthew , Board Amy , Brown Catherine , DeMaria Alfred , Buchacz Kate 
Published: November 2019

Objectives. To describe and control an outbreak of HIV infection among people who inject drugs (PWID). Methods. The investigation included people diagnosed with HIV infection during 2015 to 2018 linked to 2 cities in northeastern Massachusetts epidemiologically or through molecular analysis. Field activities included qualitative interviews regarding service availability and HIV risk behaviors. Results. We identified 129 people meeting the case definition; 116 (90%) reported injection drug use. Molecular surveillance added 36 cases to the outbreak not otherwise linked. The 2 largest molecular groups contained 56 and 23 cases. Most interviewed PWID were homeless. Control measures, including enhanced field epidemiology, syringe services programming, and community outreach, resulted in a significant decline in new HIV diagnoses. Conclusions. We illustrate difficulties with identification and characterization of an outbreak of HIV infection among a population of PWID and the value of an intensive response. Public Health Implications. Responding to and preventing outbreaks requires ongoing surveillance, with timely detection of increases in HIV diagnoses, community partnerships, and coordinated services, all critical to achieving the goal of the national Ending the HIV Epidemic initiative.
Assessing for Cardiotoxicity from Metal-on-Metal Hip Implants with Advanced Multimodality Imaging Techniques
Authors: Berber Reshid , Abdel-Gadir Amna , Rosmini Stefania , Captur Gabriella , Nordin Sabrina , Culotta Veronica , Palla Luigi , Kellman Peter , Lloyd Guy W. , Skinner John A. , Moon James C. , Manisty Charlotte , Hart Alister J. 
Published: November 2019

Background:
High failure rates of metal-on-metal (MoM) hip implants prompted regulatory authorities to issue worldwide safety alerts. Circulating cobalt from these implants causes rare but fatal autopsy-diagnosed cardiotoxicity. There is concern that milder cardiotoxicity may be common and underrecognized. Although blood metal ion levels are easily measured and can be used to track local toxicity, there are no noninvasive tests for organ deposition. We sought to detect correlation between blood metal ions and a comprehensive panel of established markers of early cardiotoxicity.

Methods:
Ninety patients were recruited into this prospective single-center blinded study. Patients were divided into 3 age and sex-matched groups according to implant type and whole-blood metal ion levels. Group-A patients had a ceramic-on-ceramic [CoC] bearing; Group B, an MoM bearing and low blood metal ion levels; and Group C, an MoM bearing and high blood metal-ion levels. All patients underwent detailed cardiovascular phenotyping using cardiac magnetic resonance imaging (CMR) with T2*, T1, and extracellular volume mapping; echocardiography; and cardiac blood biomarker sampling. T2* is a novel CMR biomarker of tissue metal loading.

Results:
Blood cobalt levels differed significantly among groups A, B, and C (mean and standard deviation [SD], 0.17 ± 0.08, 2.47 ± 1.81, and 30.0 ± 29.1 ppb, respectively) and between group A and groups B and C combined. No significant between-group differences were found in the left atrial or ventricle size, ejection fraction (on CMR or echocardiography), T1 or T2* values, extracellular volume, B-type natriuretic peptide level, or troponin level, and all values were within normal ranges. There was no relationship between cobalt levels and ejection fraction (R = 0.022, 95% confidence interval [CI] = −0.185 to 0.229) or T2* values (R = 0.108, 95% CI = −0.105 to 0.312).

Conclusions:
Using the best available technologies, we did not find that high (but not extreme) blood cobalt and chromium levels had any significant cardiotoxic effect on patients with an MoM hip implant. There were negligible-to-weak correlations between elevated blood metal ion levels and ejection fraction even at the extremes of the 95% CI, which excludes any clinically important association.

Level of Evidence:
Therapeutic Level II. See Instructions for Authors for a complete description of levels of evidence.
The Rate of Acute Kidney Injury After Total Hip Arthroplasty Is Low but Increases Significantly in Patients with Specific Comorbidities
Authors: Gharaibeh Kamel A. , Hamadah Abdurrahman M. , Sierra Rafael J. , Leung Nelson , Kremers Walter K. , El-Zoghby Ziad M. 
Published: November 2019

Background:
The purpose of this study was to investigate the rate and risk factors associated with the development of acute kidney injury after total hip arthroplasty, including the perioperative use of nonsteroidal anti-inflammatory drugs (NSAIDs).

Methods:
We retrospectively collected the demographic and comorbidity data of all patients who underwent total hip arthroplasty between 2004 and 2014 at our institution (n = 8,949). We conducted analyses of the entire cohort and a nested case-control subset. Subjects who developed acute kidney injury were matched by age, sex, and year of surgical procedure to subjects without acute kidney injury. Variables associated with acute kidney injury were determined using univariate and multivariate logistic regressions.

Results:
The mean patient age (and standard deviation) was 64.6 ± 13.8 years, 48.6% of patients were male, and 114 cases (1.1%) developed acute kidney injury, mostly stage 1 (79%). Variables associated with acute kidney injury included older age (odds ratio [OR], 1.4 per decade; p < 0.001), male sex (OR, 1.78; p = 0.005), chronic kidney disease (OR, 4.6; p < 0.001), heart failure (OR, 4.5; p < 0.001), diabetes (OR, 2.1; p < 0.001), and hypertension (OR, 2.1; p = 0.007). The results were consistent in the case-control analysis. NSAIDs were not associated with acute kidney injury (OR, 1.26; p = 0.36), but were avoided in subjects at risk, making any interpretation difficult because of confounding. A risk model for acute kidney injury after total hip arthroplasty was developed for clinical use and had good discrimination (area under the curve, 0.82; p < 0.001).

Conclusions:
The rate of acute kidney injury after total hip arthroplasty is low, but increases significantly, from <1% to >20%, in those with several independent risk factors present preoperatively. Increasing awareness of these risk factors may help to decrease the risk of acute kidney injury after total hip arthroplasty.

Level of Evidence:
Therapeutic Level III. See Instructions for Authors for a complete description of levels of evidence.
Pulsatile Lavage of Musculoskeletal Wounds Causes Muscle Necrosis and Dystrophic Calcification in a Rat Model
Authors: Chiaramonti Alexander M. , Robertson Astor D. , Nguyen Thao P. , Jaffe David E. , Hanna E. Lex , Holmes Robert , Barfield William R. , Fourney William L. , Stains Joseph P. , Pellegrini Vincent D. 
Published: November 2019

Background:
Adequate irrigation of open musculoskeletal injuries is considered the standard of care to decrease bacterial load and other contaminants. While the benefit of debris removal compared with the risk of further seeding by high-pressure lavage has been studied, the effects of irrigation on muscle have been infrequently reported. Our aim in the present study was to assess relative damage to muscle by pulsatile lavage compared with bulb-syringe irrigation.

Methods:
In an animal model of heterotopic ossification, 24 Sprague-Dawley rats underwent hindlimb blast amputation via detonation of a submerged explosive, with subsequent through-the-knee surgical amputation proximal to the zone of injury. All wounds were irrigated and underwent primary closure. In 12 of the animals, pulsatile lavage (20 psi [138 kPa]) was used as the irrigation method, and in the other 12 animals, bulb-syringe irrigation was performed. A third group of 6 rats did not undergo the blast procedure but instead underwent surgical incision into the left thigh muscle followed by pulsatile lavage. Serial radiographs of the animals were made to monitor the formation of soft-tissue radiopaque lesions until euthanasia at 6 months. Image-guided muscle biopsies were performed at 8 weeks and 6 months (at euthanasia) on representative animals from each group. Histological analysis was performed with hematoxylin and eosin, alizarin red, and von Kossa staining on interval biopsy and postmortem specimens.

Results:
All animals managed with pulsatile lavage, with or without blast injury, developed soft-tissue radiopaque lesions, whereas no animal that had bulb-syringe irrigation developed these lesions (p = 0.001). Five of the 12 animals that underwent blast amputation with pulsatile lavage experienced wound complications, whereas no animal in the other 2 groups experienced wound complications (p = 0.014). Radiopaque lesions appeared approximately 10 days postoperatively, increased in density until approximately 16 weeks, then demonstrated signs of variable regression. Histological analysis of interval biopsy and postmortem specimens demonstrated tissue damage with inflammatory cells, cell death, and dystrophic calcification.

Conclusions:
Pulsatile lavage of musculoskeletal wounds can cause irreversible insult to tissue, resulting in myonecrosis and dystrophic calcification.

Clinical Relevance:
The benefits and offsetting harm of pulsatile lavage (20 psi) should be considered before its routine use in the management of musculoskeletal wounds.