bluuebunny/crossref_metadata_2025_split huggingface dataset:
Phosphoproteomic identification of vasopressin‐regulated protein kinases in collecting duct cells
Authors: Datta Arnab , Yang Chin‐Rang , Salhadar Karim , Park Euijung , Chou Chung‐Lin , Raghuram Viswanathan , Knepper Mark A. 
Published: December 2020

Background and PurposeThe peptide hormone vasopressin regulates water transport in the renal collecting duct largely via the V2 receptor, which triggers a cAMP‐mediated activation of a PKA‐dependent signalling network. The protein kinases downstream from PKA have not been fully identified or mapped to regulated phosphoproteins.Experimental ApproachWe carried out systems‐level analysis of large‐scale phosphoproteomic data quantifying vasopressin‐induced changes in phosphorylation in aquaporin‐2‐expressing cultured collecting duct (mpkCCD) cells. Quantification was done using stable isotope labelling (SILAC method).Key ResultsNine thousand six hundred forty phosphopeptides were quantified. Stringent statistical analysis identified significant changes in response to vasopressin in 429 of these phosphopeptides. The corresponding phosphoproteins were mapped to known vasopressin‐regulated cellular processes. The vasopressin‐regulated sites were classified according to the sequences surrounding the phosphorylated amino acids giving 11 groups. Among the vasopressin‐regulated phosphoproteins were 25 distinct protein kinases. Among these, six plus PKA appeared to account for phosphorylation of about 81% of the 313 vasopressin‐regulated phosphorylation sites. The six downstream kinases were salt‐inducible kinase 2 (Sik2), cyclin‐dependent kinase 18 (Cdk18), calmodulin‐dependent kinase kinase 2 (Camkk2), protein kinase D2 (Prkd2), mitogen‐activated kinase 3 (Mapk3) and myosin light chain kinase (Mylk).Conclusion and ImplicationsIn V2 receptor‐mediated signalling, PKA is at the head of a complex network that includes at least six downstream vasopressin‐regulated protein kinases that are prime targets for future study. The extensive phosphoproteomic data reported in this study are provided as a web‐based data resource for future studies of GPCRs. [Correction added on 4 March 2021, after first online publication: The first sentence in the Key Results was corrected in this current version.]
Primary cutaneous malignant melanoma in Rett syndrome: Report of a case with nuclear features resembling herpes simplex virus cytopathic effects—a hitherto unrecognized morphological variant
Authors: Petkar Mahir Abdulla , Rodrigo Thushara 
Published: December 2020

AbstractRett syndrome (RTT) is a progressive neurological disorder, affecting females with mutations in the X‐linked gene methyl‐CpG‐binding protein 2 (MECP2). While MECP2 has been implicated in cancers of the breast, colon, and prostrate, cancer in patients with RTT is rare. We present a case of malignant melanoma in a patient with RTT, which additionally, displayed hitherto undescribed nuclear features, resembling herpes simplex virus cytopathic effects.
Risk factors in early life for developmental coordination disorder: a scoping review
Authors: van Hoorn Jessika F , Schoemaker Marina M , Stuive Ilse , Dijkstra Pieter U , Rodrigues Trigo Pereira Francisca , van der Sluis Corry K , Hadders‐Algra Mijna 
Published: December 2020

AimTo perform a scoping literature review of associations between risk factors in early life and developmental coordination disorder (DCD).MethodPubMed, Embase, CINAHL, PsycINFO, and Web of Science (January 1994–March 2019) were searched to identify studies on early risk factors and motor impairment or DCD. The effect of single and multiple risk factors was assessed. Level of evidence was evaluated following the Centre for Evidence‐Based Medicine guidelines. Meta‐analysis on the effect of preterm birth was performed.ResultsThirty‐six studies fulfilled inclusion criteria; 35 had evidence level 3, one had level 4. Highest evidence was available that preterm birth and male sex in term‐born children were associated with DCD. The odds ratio of preterm birth was 2.02 (95% confidence interval: 1.43–2.85). Low to moderate evidence was available that parental subfertility, maternal smoking during pregnancy, postnatal corticosteroid treatment in infants born preterm, extra corporeal membrane oxygenation, retinopathy of prematurity, abnormalities on magnetic resonance imaging scans at term age, and accumulating perinatal or neonatal risk factors were associated with motor impairment.InterpretationLimited information on early risk factors of DCD is available. Only preterm birth and male sex were consistently associated with an increased risk of DCD.What this paper adds

Preterm birth is a risk factor for developmental coordination disorder (DCD).

In term‐born children, male sex was consistently associated with DCD.

Risk factors for DCD are similar to risk factors for cerebral palsy.
Unique technique to relieve left ventricular assist device electromagnetic interference with an implantable cardioverter defibrillator
Authors: Jin Chengyue , Hsu Joshua , Frenkel Daniel , Jacobson Jason T. , Iwai Sei , Ferrick Aileen 
Published: December 2020

AbstractWe introduced a simple technique to eliminate electromagnetic interference between a left ventricular assist device (LVAD) and an implantable cardioverter defibrillator (ICD). A 43‐year‐old male with heart failure and a reduced ejection fraction who had an ICD presented with decompensated heart failure and received an LVAD as a bridge to transplant. Remote monitoring showed persistent atrial fibrillation causing an inappropriate ICD shock leading to a decision to disable shock therapies. However, an in‐office interrogation was unsuccessful due to electromagnetic interference. Patient was instructed to extend his arm above his head on the ipsilateral side of the ICD, thus increasing the distance between LVAD and ICD, eliminating the interaction to allow reprogramming of the device.
Exosome‐derived long non‐coding RNA ADAMTS9‐AS2 suppresses progression of oral submucous fibrosis via AKT signalling pathway
Authors: Zhou Shanghui , Zhu Yun , Li Zhenming , Zhu Yonggan , He Zhijing , Zhang Chenping 
Published: December 2020

AbstractOral submucosal fibrosis (OSF) is one of the pre‐cancerous lesions of oral squamous cell carcinoma (OSCC). Its malignant rate is increasing, but the mechanism of malignancy is not clear. We previously have elucidated the long non‐coding RNA (lncRNA) expression profile during OSF progression at the genome‐wide level. However, the role of lncRNA ADAMTS9‐AS2 in OSF progression via extracellular communication remains unclear. lncRNA ADAMTS9‐AS2 is down‐regulated in OSCC tissues compared with OSF and normal mucous tissues. Low ADAMTS9‐AS2 expression is associated with poor overall survival. ADAMTS9‐AS2 is frequently methylated in OSCC tissues, but not in normal oral mucous and OSF tissues, suggesting tumour‐specific methylation. Functional studies reveal that exosomal ADAMTS9‐AS2 suppresses OSCC cell growth, migration and invasion in vitro. Mechanistically, exosomal ADAMTS9‐AS2 inhibits AKT signalling pathway and regulates epithelial‐mesenchymal transition markers. Through profiling miRNA expression profile regulated by exosomal ADAMTS9‐AS2, significantly enriched pathways include metabolic pathway, PI3K‐Akt signalling pathway and pathways in cancer, indicating that exosomal ADAMTS9‐AS2 exerts its functions through interacting with miRNAs during OSF progression. Thus, our findings highlight the crucial role of ADAMTS9‐AS2 in the cell microenvironment during OSF carcinogenesis, which is expected to become a marker for early diagnosis of OSCC.
CASC9 plays a role in salivary adenoid cystic carcinoma in vitro by upregulation of ACLY
Authors: Xie Hongliang , Tang Jianming , Lu Lu , Li Bohan , Wang Mengmeng 
Published: December 2020

AbstractObjectiveThe study was designed to explore the role of cancer susceptibility candidate 9 (CASC9) in salivary adenoid cystic carcinoma (SACC) (SACC‐83 and SACC‐LM) cell malignant phenotypes.MethodsColony formation assay was used to measure cell proliferation. Transwell assay was used to detect cell migration and invasion. Flow cytometry analysis was applied to determine cell cycle distribution and apoptosis. FISH assay revealed the subcellular location of CASC9.ResultsDownregulation of CASC9 inhibited SACC cell proliferation, migration, and invasion, led to cell arrest at G0/G1 phase, and facilitated cell apoptosis. In mechanism, CASC9 bound with microRNA 146b‐5p (miR‐146b‐5p) and negatively modulated miR‐146b‐5p expression. MiR‐146b‐5p directly targeted 3’ untranslated region of ATP‐Citrate Lyase (ACLY) to degrade ACLY in SACC cells. CASC9 upregulated ACLY expression through competitively binding with miR‐146b‐5p. Furthermore, rescue assays indicated that ACLY overexpression counteracted the effects triggered by CASC9 knockdown on cell proliferation, migration, invasion, and apoptosis in SACC cells.ConclusionCASC9 facilitated the malignant phenotypes of SACC cells by the regulation of the miR‐146b‐5p/ACLY axis. These findings might lay foundation for SACC research.
Forearm Interosseous Membrane Maintains the Stability of Proximal Radioulnar Joint
Authors: Zhang Ning , Fang Jia‐hu 
Published: December 2020

ObjectiveTo evaluate the effect of the proximal and central bundles of the interosseous membrane on the stability of proximal radioulnar joint.MethodsTwenty fresh samples of human forearm provided by the anatomy room of the Department of Human Anatomy of Nanjing Medical University were included in this study. They were used to explore the effect of proximal interosseous membrane bundle on the stability of proximal radioulnar joint. The proximal bundle was reconstructed along the original attachment point. The reconstructions of central bundle were divided into the reconstruction of original attachment point on radius‐midpoint of the ulnar original attachment point (reconstruction A) and original attachment point reconstruction (reconstruction B). The loads of the proximal radioulnar joint in different positions were measured. The load of the proximal radioulnar joint was analyzed in neutral, pronation, and supination positions.ResultsAfter resection of proximal and central fascicles, the loads of proximal radioulnar joint in neutral, pronation, and supination positions were significantly lower than those before resection (P < 0.05). After reconstruction, the loads of proximal radioulnar joint in neutral and supination positions were higher than those after resection (P < 0.05). After reconstruction, the loads of proximal radioulnar joint in neutral and supination positions were higher than those after resection (P < 0.05), and that after reconstruction B in pronation position was higher than that after resection (P < 0.05), while there was no significant difference between reconstruction A and after resection (P > 0.05). In supination position, the load of reconstruction B was higher than that of reconstruction A (P < 0.05). After reconstruction of the proximal and central bundles, the proximal radioulnar joint could not reached the same load as it could before resection (P < 0.05).ConclusionThe stability of proximal radioulnar joint is affected by central bundle and proximal bundle. Reconstruction can increase the stability of proximal radioulnar joint.
Sterile Tissue Ablation Using Laser Light—System Design, Experimental Validation, and Outlook on Clinical Applicability
Authors: Duverney Cédric , Abbasi Hamed , Berkelaar Majoska , Pelttari Karoliina , Cattin Philippe C. , Barbero Andrea , Zam Azhar , Rauter Georg 
Published: December 2020

Abstract
Preparation of biological samples for further processing or analysis is generally performed manually by means of standard mechanical tools such as scalpels or biopsy punches. While this approach is uncomplicated and swift, it entails constraints such as low, operator-dependent cutting accuracy and reproducibility. Tissue segments surrounding the cut may further suffer mechanical and thermal damage due to shear forces and friction between tool and sample. These hindrances affect procedures both in the laboratory environment as well as within clinical settings. A system has been developed leveraging robotic positioning and laser light for precise, controlled, and contactless tissue ablation, and providing a concise and intuitive graphical user interface. Additionally, sterility of the process is demonstrated, a paramount element for clinical application. The proposed process does not require sterilization of the robotic components or the lasers, easing a prospective integration into existing workflows. In the context of this work, mainly cartilage repair surgery is targeted. The proposed system allows for highly accurate and reproducible shaping of the cartilage lesion area as well as its corresponding engineered cartilage graft, possibly leading to better and faster integration at the defect site. Promising results could be obtained in a first test series with human cartilage samples, validating the functionality of the preparation system and the feasibility of the sterility concept.
Thermal properties of $$\pi $$ and $$\rho $$ meson
Authors: Gao Fei , Ding Minghui 
Published: December 2020

AbstractWe computed the pole masses and decay constants of $$\pi $$
π
and $$\rho $$
ρ
meson at finite temperature in the framework of Dyson–Schwinger equations and Bethe–Salpeter equations approach. Below transition temperature, pion pole mass increases monotonously, while $$\rho $$
ρ
meson seems to be temperature independent. Above transition temperature, pion mass approaches the free field limit of screening mass $$\sim 2\pi T$$

∼
2
π
T

, whereas $$\rho $$
ρ
meson is about twice as large as that limit. Pion and the longitudinal projection of $$\rho $$
ρ
meson decay constants have similar behaviour as the order parameter of chiral symmetry, whereas the transverse projection of $$\rho $$
ρ
meson decay constant rises monotonously as temperature increases. The inflection point of decay constant and the chiral susceptibility get the same phase transition temperature. Though there is no access to the thermal width of mesons within this scheme, it is discussed by analyzing the Gell-Mann-Oakes-Renner (GMOR) relation in medium. These thermal properties of hadron observables will help us understand the QCD phases at finite temperature and can be employed to improve the experimental data analysis and heavy ion collision simulations.
Direct Admission of Patients With ST‐Segment–Elevation Myocardial Infarction to the Catheterization Laboratory Shortens Pain‐to‐Balloon and Door‐to‐Balloon Time Intervals but Only the Pain‐to‐Balloon Interval Impacts Short‐ and Long‐Term Mortality
Authors: Meisel Simcha R. , Kleiner‐Shochat Michael , Abu‐Fanne Rami , Frimerman Aaron , Danon Asaf , Minha Sa’ar , Levi Yaniv , Blatt Alex , Mohsen Jameel , Shotan Avraham , Roguin Ariel 
Published: December 2020

Background
Shortening the pain‐to‐balloon (P2B) and door‐to‐balloon (D2B) intervals in patients with ST‐segment–elevation myocardial infarction (STEMI) treated by primary percutaneous coronary intervention (PPCI) is essential in order to limit myocardial damage. We investigated whether direct admission of PPCI‐treated patients with STEMI to the catheterization laboratory, bypassing the emergency department, expedites reperfusion and improves prognosis.

Methods and Results

Consecutive PPCI‐treated patients with STEMI included in the ACSIS (Acute Coronary Syndrome in Israel Survey), a prospective nationwide multicenter registry, were divided into patients admitted directly or via the emergency department. The impact of the P2B and D2B intervals on mortality was compared between groups by logistic regression and propensity score matching. Of the 4839 PPCI‐treated patients with STEMI, 1174 were admitted directly and 3665 via the emergency department. Respective median P2B and D2B were shorter among the directly admitted patients with STEMI (160 and 35 minutes) compared with those admitted via the emergency department (210 and 75 minutes,
P
<0.001). Decreased mortality was observed with direct admission at 1 and 2 years and at the end of follow‐up (median 6.4 years,
P
<0.001). Survival advantage persisted after adjustment by logistic regression and propensity matching. P2B, but not D2B, impacted survival (
P
<0.001).

Conclusions
Direct admission of PPCI‐treated patients with STEMI decreased mortality by shortening P2B and D2B intervals considerably. However, P2B, but not D2B, impacted mortality. It seems that the D2B interval has reached its limit of effect. Thus, all efforts should be extended to shorten P2B by educating the public to activate early the emergency medical services to bypass the emergency department and allow timely PPCI for the best outcome.
Fātiḥa Marriage in Morocco
Authors: Yavuz-Altıntaş Miyase 
Published: December 2020

Abstract
This paper investigates the debates over, and the promulgation of, the new Moroccan laws on unregistered customary marriages and on establishing the paternity of offspring resulting from such marriages, and it analyzes how those laws have been implemented by the judiciary. The paper closely examines the relevant deliberations of the Moroccan Royal Advisory Commission, and analyzes 24 court cases involving the laws. I argue that, while monogamous registered marriage is depicted in the national legal system as the basis for establishing a modern Moroccan society, legislators regard fātiḥa marriage as a social reality that has its roots in customs and religious practices. The paper shows that judges abide by the conditions specified in law but differ in their interpretation of “force majeure” when it comes to a couple’s having not registered their marriage. The study also reveals how the laws create legal loopholes in terms of underage marriage and polygyny, which are strictly restricted in the code.
SOUTHTRAC-GW: An Airborne Field Campaign to Explore Gravity Wave Dynamics at the World’s Strongest Hotspot
Authors: Rapp Markus , Kaifler Bernd , Dörnbrack Andreas , Gisinger Sonja , Mixa Tyler , Reichert Robert , Kaifler Natalie , Knobloch Stefanie , Eckert Ramona , Wildmann Norman , Giez Andreas , Krasauskas Lukas , Preusse Peter , Geldenhuys Markus , Riese Martin , Woiwode Wolfgang , Friedl-Vallon Felix , Sinnhuber Björn-Martin , Torre Alejandro de la , Alexander Peter , Hormaechea Jose Luis , Janches Diego , Garhammer Markus , Chau Jorge L. , Conte J. Federico , Hoor Peter , Engel Andreas 
Published: December 2020

AbstractThe southern part of South America and the Antarctic peninsula are known as the world’s strongest hotspot region of stratospheric gravity wave (GW) activity. Large tropospheric winds are deflected by the Andes and the Antarctic Peninsula and excite GWs that might propagate into the upper mesosphere. Satellite observations show large stratospheric GW activity above the mountains, the Drake Passage, and in a belt centered along 60°S. This scientifically highly interesting region for studying GW dynamics was the focus of the Southern Hemisphere Transport, Dynamics, and Chemistry–Gravity Waves (SOUTHTRAC-GW) mission. The German High Altitude and Long Range Research Aircraft (HALO) was deployed to Rio Grande at the southern tip of Argentina in September 2019. Seven dedicated research flights with a typical length of 7,000 km were conducted to collect GW observations with the novel Airborne Lidar for Middle Atmosphere research (ALIMA) instrument and the Gimballed Limb Observer for Radiance Imaging of the Atmosphere (GLORIA) limb sounder. While ALIMA measures temperatures in the altitude range from 20 to 90 km, GLORIA observations allow characterization of temperatures and trace gas mixing ratios from 5 to 15 km. Wave perturbations are derived by subtracting suitable mean profiles. This paper summarizes the motivations and objectives of the SOUTHTRAC-GW mission. The evolution of the atmospheric conditions is documented including the effect of the extraordinary Southern Hemisphere sudden stratospheric warming (SSW) that occurred in early September 2019. Moreover, outstanding initial results of the GW observation and plans for future work are presented.
Climate Extremes across the North American Arctic in Modern Reanalyses
Authors: Avila-Diaz Alvaro , Bromwich David H. , Wilson Aaron B. , Justino Flavio , Wang Sheng-Hung 
Published: December 2020

ABSTRACTAtmospheric reanalyses are a valuable climate-related resource where in situ data are sparse. However, few studies have investigated the skill of reanalyses to represent extreme climate indices over the North American Arctic, where changes have been rapid and indigenous responses to change are critical. This study investigates temperature and precipitation extremes as defined by the Expert Team on Climate Change Detection and Indices (ETCCDI) over a 17-yr period (2000–16) for regional and global reanalyses, namely the Arctic System Reanalysis, version 2 (ASRv2); North American Regional Reanalysis (NARR); European Centre for Medium-Range Weather Forecasts (ECMWF) ERA5 reanalysis; Modern-Era Retrospective Analysis for Research and Applications, version 2 (MERRA-2); and Global Meteorological Forcing Dataset for Land Surface Modeling (GMFD). Results indicate that the best performances are demonstrated by ASRv2 and ERA5. Relative to observations, reanalyses show the weakest performance over far northern basins (e.g., the Arctic and Hudson basins) where observing networks are less dense. Observations and reanalyses show consistent warming with decreased frequency and intensity of cold extremes. Cold days, cold nights, frost days, and ice days have decreased dramatically over the last two decades. Warming can be linked to a simultaneous increase in daily precipitation intensity over several basins in the domain. Moreover, the North Atlantic Oscillation (NAO) and Arctic Oscillation (AO) distinctly influence extreme climate indices. Thus, these findings detail the complexity of how the climate of the Arctic is changing, not just in an average sense, but in extreme events that have significant impacts on people and places.
Inferring directional relationships in microbial communities using signed Bayesian networks
Authors: Sazal Musfiqur , Mathee Kalai , Ruiz-Perez Daniel , Cickovski Trevor , Narasimhan Giri 
Published: December 2020

AbstractBackgroundMicrobe-microbe and host-microbe interactions in a microbiome play a vital role in both health and disease. However, the structure of the microbial community and the colonization patterns are highly complex to infer even under controlled wet laboratory conditions. In this study, we investigate what information, if any, can be provided by aBayesian Network(BN) about a microbial community. Unlike the previously proposedCo-occurrence Networks(CoNs), BNs are based on conditional dependencies and can help in revealing complex associations.ResultsIn this paper, we propose a way of combining a BN and a CoN to construct asigned Bayesian Network(sBN). We report a surprising association between directed edges in signed BNs and knowncolonization orders.ConclusionsBNs are powerful tools for community analysis and extracting influences and colonization patterns, even though the analysis only uses an abundance matrix with no temporal information. We conclude that directed edges in sBNs when combined with negative correlations are consistent with and strongly suggestive of colonization order.