bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association between clinical competencies and mental health symptoms among frontline medical staff during the COVID-19 outbreak: A cross-sectional study
Authors: Chang Qing , Xia Yang , Gao Shanyan , Sun Yongjun , Zhang Ming , Su Han , Ma Xiaoyu , Liu Yashu , Zhao Yuhong 
Published: July 2020

Abstract
Background: In China, mental health of frontline medical staff might be influenced by clinicians’ ability to handle the outbreak of coronavirus disease 2019 (COVID-19). Few studies to-date have addressed the association between clinicians’ competencies and mental health in this context. This cross-sectional study has examined the prevalence of mental health symptoms among frontline medical staff handling the COVID-19 outbreak, and explored the associations between their competencies, and separate and concurrent depressive and anxiety symptoms. Methods: A total of 623 frontline medical staff were included in this study, which took place from January 20, 2020 to February 20, 2020. Competencies, depressive symptoms, and anxiety symptoms were assessed using the short form of the Chinese clinical physicians’ competency model, Patient Health Questionnaire-9, and Generalized Anxiety Disorder-7 questionnaire, respectively. Logistic regression models were used to estimate the associations between one standard deviation increase in competency scores and the prevalence of mental health problems. Multivariate-adjusted odds ratios (ORs) and corresponding 95% confidence intervals (CIs) were calculated.Results: Among 623 staff members, the prevalence of depressive, anxiety, and comorbid depressive and anxiety symptoms was 40.93%, 31.78%, and 26.00%, respectively. Among the medical staff with higher total competency scores, the prevalence of depressive (OR=0.67, 95% CI: 0.55-0.81), anxiety (OR=0.68, 95% CI: 0.56-0.83), and comorbid anxiety and depressive symptoms (OR=0.69, 95% CI: 0.55-0.83) was lower than among their lower-scoring counterparts. Subgroup analyses stratified by core competency scores revealed similar associations as the main analyses.Conclusions: The present findings highlight the association between high core competency scores and lower prevalence of depressive, anxiety, and comorbid anxiety and depressive symptoms. Improving medical staff’s core competencies might help prevent mental health problems among frontline medical staff responding to COVID-19.
The Fastest Simulation of Protein Folding Based on Torsion Angles
Authors: Jeong Seonghoon 
Published: July 2020

Abstract
Backgrounds:
Enormous number of possible conformations in the protein structure simulation have led molecular dynamics researchers to be frustrated until now. Some methods with defects ended their experiments into failure. This made them fail to determine the structure and function of folded protein in stable state with the lowest potential energy. This apparently exist in nature. The purpose of resolving a protein folding pathway that follows protein backbone residues torsional inertia was accomplished.
Results
A new method, torsion angle modeling, was adopted focused on the rotation of dihedral angles. The potential energy was calculated by rotating torsion angles of the peptide with 8 residues. It was found that when moving in the order of torsional inertia, 8 residues swivel in sequence. Six passes were repeated to find the lowest value.
Conclusion
The protein backbone torsion angle plays very important role in predicting protein structure. Actually it was thousand times faster or more than others to get the obvious pathway.
Efficacy and safety of knee flexion enhanced standardized home exercise program comparing supervised physiotherapy to improve mobility and quality of life after Total knee arthroplasty
Authors: Xu Tianyang , Fan Lin , Yang Dong , Liu Kaiyuan , Gao Qiuming , Li Guodong 
Published: July 2020

Abstract
Background: In the present study, we introduced a knee flexion enhanced standardized home exercise program assisted by a low stool and evaluate efficiency of this method in the management of rehabilitation after TKA.Methods: Patients were recruited from July 2014 and December 2015, and randomized into two groups, a knee flexion enhanced standardized home exercise program group (group KFEH) and commonly-used supervised physiotherapy group (group SPT). Outcomes were evaluated by observing the joint function with the Knee Society score (KSS), Visual Analogue Scale (VAS), the Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) score, and ROM assessment at selected time points (pre-operation and 1 week, 1 month, 3 month, 6 month and 1 year following post-operation). Results: At last, we observed pain and functional improvements in both groups. Noninferiority was shown 12 months postoperatively for clinical outcomes except group KFEH had better ROM range at 1 month (P<0.01). The absolute value of WOMAC and KSS were slight better in group KFEH without significant difference, and there was no difference about VAS between two groups as well as complication incidence rate. Additionally, home program would save patients’ times and decrease economic burden. Patents would save 14 times postoperative rehabilitation in outhospital service, and cut down at least 782 RMB in total. Conclusion: Taking the rehabilitation and economical efficiency, we recommend knee flexion enhanced home exercise program for TKA rehabilitation management.
Three-column Subdivision for Isolated Posterolateral Tibial Plateau Fractures and Perspective Surgical Approachs
Authors: Sim Jae Ang , Park Ji Hyeon , Na Young Gon , Lee Byung Hoon 
Published: July 2020

Abstract
Background: Management of the tibial plateau fracture involving posterolateral compartment is technically challenging. This study aimed to introduce a computed tomography-based classification of the posterolateral compartment of tibial plateaus based on the fibula and to propose the individualized surgical approaches for each zone; and 2) to determine the surgical approach based on the classification, would achieve a safe and effective reduction and can improve postoperative clinical outcomes.Methods: Eighteen cases of tibia plateau fracture involved the isolated posterolateral compartment in a single institution were retrospectively analyzed. The posterolateral compartment of tibial plateau was segmented into three zones based on the fibular position and individualized surgical approach was proposed for each zone. In anterior Zone I, surgical treatment was performed using an extended anterolateral approach and patient was placed in the supine position; In middle Zone II, using the transfibular approach in the supine position; In posterior Zone III, using the posteromedial approach in prone. Articular reduction (intra-articular step off in CT images) and mechanical medial proximal tibial angle (mMPTA) in simple radiographs taken in the immediate postoperative period and clinical outcomes of the Lysholm knee scoring scale and range of motion were evaluated at postoperative 1-year follow-up,Results: In all cases, anatomical articular reduction (intra-articular step off < 2mm) was achieved, maintained for the follow-up period. The average mMPTA was increased from 87.6º before surgery to 88.2º in immediate postoperative period (p = 0.060), and maintained for the follow-up period (mean 89.9º at one-year follow-up). At the 1-year follow-up, the knee range of motion averaged 140 degrees and the Lysholm knee function scored 95.0 points.Conclusion: An individualized surgical approach and fixation according to three-zone subdivision for isolated posterolateral tibial plateau fractures provided an effective and safe method to treat posterolateral tibial plateau fractures. Level of evidence: Therapeutic study, IV
Experience of N-acetylcysteine airway management in the successful treatment of one case of critical condition with COVID-19
Authors: Liu Yan , Luo Guoshi , Qian Xin , Wu Chenglin , Tang Yijun , lu kun , Chen Biyu , Leung Elaine Lai-Han , Wang Meifang 
Published: July 2020

Abstract
Object: To report the successful diagnosis and treatment of a patient with critical condition of novel coronavirus pneumonia (COVID-19) and to summarize its clinical features and airway management experience in successful treatment.Methods: Retrospectively analyzed the successful management of one case of COVID-19 with critical condition combined respiratory failure and discussed the clinical characteristics and airway management of the patient in conjunction with a review of the latest literature.Results: A patient with an anastomotic fistula after radical treatment of esophageal cancer and right-side encapsulated pyopneumothorax was admitted with cough and dyspnea and was diagnosed with novel coronavirus pneumonia and malnutrition by pharyngeal swab nucleic acid test in combination with chest CT. The patient was treated with antibiotics, antiviral and antibacterial medications, respiratory support, expectorant nebulization, and nutritional support, expressed progressive deterioration. Endotracheal intubation and mechanical ventilation were performed since the onset of the type Ⅱ respiratory failure on the 13th day of admission. The patient had persistent refractory hypercapnia after mechanical ventilation. Based on the treatment mentioned above, combined with repeated bronchoalveolar lavage by using N-acetylcysteine ​​(NAC) inhalation solution, the patient's refractory hypercapnia was gradually improved. It was cured and discharged after being given the mechanical ventilation for 26 days as well as 46 days of hospitalization, currently is surviving well.Conclusion: Patients with severe conditions of novel coronavirus pneumonia often encounter bacterial infection in their later illness-stages. They may suffer respiratory failure and refractory hypercapnia that is difficult to improve due to excessive mucus secretion leading to small airway obstruction. In addition to the use of reasonable antibiotics and symptomatic respiratory support and other treatment, timely artificial airway and repeated bronchoalveolar NAC inhalation solution lavage, expectorant and other airway management are essential for such patients.
Study of Structural and Magnetic Properties, Trivalent Cation Substitution of Cobalt base Spinel Ferrites CoCr0.04LaxFe1.96-xO4 (0 ≤ x ≤ 0.12)
Authors: MUSTAFA G. , FAROOQ Z. , AHMAD M.R. , ANWAR H. , AKHTAR H. , YASEEN M. , QAYYUM M. A. , MAHMOOD MIAN H.R. 
Published: July 2020

Abstract
In this research work a series of polycrystalline trivalent cation (Cr3+, La3+) substituted cobalt ferrite with general formula CoCr0.04LaxFe1.96-xO4 where 0.00 ≤ x ≤ 0.12 (in steps of 0.02) was synthesized using co-precipitation method and were sintered at 900°C for 6 hours. The phase identification and confirmation of the structure were confirmed employing the X-ray diffraction (XRD) and FTIR techniques. The size of crystallite was found on average, in the range of 53-106 nano-meters. FTIR results confirmed spinel ferrites structure. Moreover the morphological studies were observed through SEM. The elemental analysis of the samples was done by EDX while, I-V characterization of the represented sample was recorded using two probe method. The measured electrical resistivity of materials increased as dopant content increased. The magnetic behavior of the materials was studied using vibrating sample magnetometer (VSM). The saturation magnetization values and magnetic coercivity values decreased with the increase of La3+ concentration. The saturation magnetization (Ms) of the ferrite materials decreased with the reduction of size.
Effectiveness of Theory of Planned Behavior-Based Educational Intervention on Newborn Care in Pregnant Mothers: A quasi-experimental study
Authors: Navabi Azamalsadat , shamsi mohsen , Khorsandi Mahboobeh , Zamanian Maryam 
Published: July 2020

Abstract
Background and Purpose: The neonatal period is one of the most sensitive stages of life, requiring proper understanding and attentive care. Accordingly, this study aimed to investigate the effectiveness of theory of planned behavior-based educational intervention on newborn care in pregnant mothers. Methodology: This study was conducted as a quasi-experimental research on 100 pregnant mothers who were randomly assigned to two groups of intervention (N=50) and control (N=50). The data were collected using a theory of planned behavior-based questionnaire. Considering the effective constructs of this theory, the educational intervention was performed in the form of four 60-minute education sessions in one month in the intervention group. One month after delivery, the data were recollected from both groups and analyzed using statistical tests.Findings: The mean and standard deviation of the mothers’ age in both intervention and control groups were 26.14±4.7 and 26.10±5.19, respectively (p=0.48). Before the educational intervention, the behavioral intention of the intervention group was 3.33 (out of 5), which significantly increased to 4.54 after the educational intervention (p<0.001). In addition, the performance of mothers regarding neonatal care was significantly enhanced from 2.86 to 4.64 one month after the delivery (p=0.001). Conclusion: The theory of planned behavior-based educational intervention, along with active follow-ups, led to better and more principled behavioral intention and care provided by pregnant mothers to their newborn infants. Therefore, providing theory of planned behavior-based education in other healthcare centers is recommended.
A Positive Feedback Regulation Involving PVT1 and HER2/hER3 Promotes Progression of High-grade Serous Ovarian Cancer
Authors: wang Wenxiang , Fan Wenqiang , Gao Yuxia , Liu Yu , Li Li , Yi Lisha , He Qian , Hai Jing , Dong Xuecai , Chen Caixia 
Published: July 2020

Abstract
Background

In recent years, long noncoding RNAs (lncRNAs) have been reported frequently to play important roles in specific cancers, including high-grade serous ovarian cancer (HGSOC). PVT1 is an important oncogenic lncRNA highly expressed in various cancers. However, little is known about the role of PVT1 in HGSOC and underlying mechanisms.
Methods

The expression levels of PVT1 and HER2/3 in HGSOC tissue and adjacent normal tissue were determined by qRT-PCR. MTT and transwell assays were used to identify the effects of PVT1 cell proliferation and migration respectively. Dual-luciferase reporter assay and RIP experiment were carried out to verify target genes of PVT1. ChIP experiment was used to identify that HER2 was transcription factor of PVT1.
Results

Our results showed that PVT1 expression was up-regulation in human HGSOC specimens and promoted ovarian cancer cell proliferation and migration. We further validated that HER2 was a direct transcription factor for facilitating PVT1 expression. In return, PVT1 enhanced HER2 transcript stability. Moreover, bioinformatics analysis and dual luciferase reporter assay results uncovered that PVT1 functioned as a competing endogenous RNA (ceRNA) for miR-1301-3p to promote cell proliferation and migration by increasing HER3 expression.
Conclusions

Taken together, our results showed that PVT1, controlled by HER2, elevated HER2 and HER3 expression to promote HGSOC progression. Thus, PVT1 can be regarded as a vital diagnostic biomarker for HGSOC and a potential novel therapeutic target.
Ethical Decision-Making of Health Professionals Caring for HIV-Infected People in Hunan, China: A Qualitative Study
Authors: Huang Hangyu , Koniak-Griffin Deborah , Wang Honghong , Yang Min 
Published: July 2020

Abstract
Background: Numerous ethical issues surged the moment AIDS was discovered. As advocates of HIV-infected people, health professionals encounter many ethical dilemmas in clinical practice. However, it remains unclear how health professionals solve these issues. To explore the ethical decision-making experiences of health professionals who care for HIV-infected people in Hunan Province, China, and to discuss the strategies for enhancing ethical decision-making competence in AIDS care. Methods: This descriptive qualitative research adopted semi-structured, in-depth interview and thematic analysis. Participants were recruited by purposive sampling. In total, twenty-two participants completed the interview. Results: Three themes emerged from data analyses of the interviews: (1) common ethical dilemma experienced by health professionals, (2) factors influencing ethical judgment, (3) ethical motivations. Conclusion: About two thirds of informants failed to recall ethical dilemmas experienced in their clinical practice. Emotions, gender, occupation, and difficulty balancing different roles may influence the ethical judgments of health professionals. In the ethical decision-making process, most informants took other people’s interests into consideration and conformed to law and professional codes of conduct. However, negative attitudes towards HIV-infected people still exist among a few professionals. Furthermore, some informants showed a misunderstanding of ethical principles. And the fear of medical disputes (conflicts with families and others) was experienced by many informants, influencing their ethical behaviors. Hence, more efforts are needed to eliminate negative attitudes towards HIV-infected patients. This study underscores the importance of continuing ethical education for HIV/AIDS-related professionals to enhance their ethical decision-making competence. Moreover, sound governmental laws may promote ethical behavior in AIDS care.
Screening and identifcation of differentially expressed microRNAs in diffuse large B-cell lymphoma based on microRNA microarray
Authors: Gao Hai-Xia , Li Xinxia 
Published: July 2020

Abstract
We aimed to screen and identify differentially expressed microRNAs (miRNAs) between diffuse large B-cell lymphoma (DLBCL) and control (lymph node reactive hyperplasia, LRH) groups, investigate whether miRNAs associated with DLBCL and could serve as potential therapeutic targets. Five DLBCL experimental samples and five control samples were obtained from fresh tissues. The exclusion criteria were expressed as follows: patients with other lymphoid diseases and patients undergoing chemical treatment. Firstly, fresh samples were analyzed using microRNA microarray to identify differentially expressed miRNAs. Next, three databases (Targetscan, microRNAorg, PITA) were used to intersection predict the potential target gene of the 204 differential miRNAs and performed Venn diagram of the results. Then, target genes of differential miRNAs were performed by Gene ontology (GO) and Kyoto Encyclopedia of Gene and Genome (KEGG) pathway analysis. Finally, to validate the microRNA microarray data, we performed Real-time reverse transcription PCR (qRT-PCR) for eight differentially expressed miRNAs (miR-193a-3p, miR-19a-3p, miR-19b-3p, miR-370-3p, miR-1275, miR-490-5p, miR-630 and miR-665) using DLBCL and LRH fresh samples. Two hundred and four miRNAs showed differential expression, including 105 down-regulated and 54 up-regulated miRNAs. The cut-off criterion was set as P value ≤0.05 and fold change (FC) ≥2. A total of 7522 potential target genes for the 204 miRNAs were predicted. Potential target genes were enriched in pathway in cancers, MAPK signal pathway, regulation of actin cytoskeleton, focal adhesion, endocytosis, Wnt signal pathway, axon guidance, calcium signal pathway, and PI3K/AKT signal pathway. Eight miRNAs validation by qRT-PCR, four miRNAs (miR-19b-3p, miR-193a-3p, miR-370-3p and miR-490-5p) were low expression in DLBCL (P< 0.05). The high expression of miR-630 in DLBCL was statistically significant (P< 0.05). Our results of identification of differentially expressed miRNAs, predicted the target gene of the differential miRNAs, enrichment of target gene biological functions and signaling pathway, will provide basis for researchers in identifying the pathogenesis of DLBCL, could serve as reliable biomarkers for precise diagnosis and as therapeutic target for improvement of therapeutic efficacy in DLBCL in the future.
Accuracy of Ultrasound Estimation of Fetal Weight at Three Teaching Hospitals in Addis Ababa
Authors: Ibrahim Alexander Mohamed , Weldetensaye Eskinder Kebede 
Published: July 2020

Abstract
BackgroundUltrasound examination and measurement of fetal weight have become an integral part of modern obstetric care. Knowing the accuracy of the ultrasound estimation of fetal weight (EFW) and what factors affect it helps make a more informed decision. Fetal weight estimation is thought to help predict fetal survival and make management decisions in the low birth weight group and in managing the delivery of the large baby, where complications may occur.ObjectiveTo assess the accuracy of ultrasound estimation of fetal weight.MethodsThe study design was a facility-based prospective cross-sectional study comparing sonographic EFW with actual birth weight, involving 384 women admitted to the study hospitals. And assessed 12 factors that might affect accuracy (place of delivery/scan, level of the operator, time of scan (working vs. non-working hours), fetal presentation, liquor volume, placental location, days passed between scan and delivery, time of the scan to labor (antepartum vs. intrapartum), fetal sex, maternal BMI, gestational age, and fetal weight. Data were collected using structured questionnaires, developed based on study variables and available literature, and analyzed using SPSS software.ResultThe EFW that is within 10% of the actual birth weight is 62.4%. The overall mean error of EFW was 9.1%, with an SD of ± 7.1%. Of the 12 factors assessed, it was found that the place of delivery/scan (p = 0.017) and the level of resident experience (p = 0.47) significantly affected the accuracy. When R4 was compared to R1’s OR = 4.656(95%CI, [1.111–19.506], p = 0.035) and R2’s OR = 2.112(95%CI,[1.062–4.201],p = 0.033) but not significant when compared to R3’s OR = 1.227(95%CI,[0.563–1.191],p = 0.607). A paired sample t-test was applied (P = 0.14).Conclusion and recommendationThe EFW of R4’s was significantly different from that of R1’s and R2’s, but not R3’s we recommend that all ultrasound scans whose weight estimation can affect the management plan of the patient to be done by the senior residents rather than the junior residents i.e. the R3’s and R4’s.
Subcellular Localization of Cerebellar Degeneration-Related Protein 1
Authors: Solheim Eirik Tveit , Kråkenes Torbjørn , Totland Cecilie , Vedeler Christian Alexander 
Published: July 2020

Abstract
Background: Cerebellar degeneration-related protein 1 (CDR1) is associated with paraneoplastic cerebellar degeneration – an autoimmunedisease characterized by neurodegeneration in the cerebellum. Paraneoplastic syndromes are believed to be triggered by ectopic tumour expression of neuronal proteins. The CDR1 protein is expressed by neurons in the cerebellar cortex and in certain cancers. In this study, we characterized the subcellular localization of CDR1 to help delineate potential functions in cancer cells and neurons. Methods: Ovarian cancer cells, cerebellar sections, and Purkinje cells in culture were immunostained and imaged by super-resolution stimulated emission depletion microscopy and potential interaction partners were investigated using a proximity ligation assay. Results: CDR1 was localized to the mitochondria in cancer cells and was colocalized with proteins of the outer mitochondrial membrane. Using a proximity ligation assay, we discovered that CDR1 interacts with mitofusin 1 – a transmembrane GTPase involved in mitochondrial fusion – in both cancer cells and rat cerebellum. CDR1 also colocalized with intermediate filaments, including vimentin, in cancer cells and with neurofilaments in human cerebellum. Conclusion: The dual localization to mitochondria and intermediate filaments as well as colocalization with key proteins involved in mitochondrial dynamics suggest that CDR1 is involved in the subcellular distribution of mitochondria in cells.