bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of close up antimicrobial therapies for treatment and prevention of subclinical mastitis in the herds with high prevalence of Staphylococcus aureus
Authors: Amiri Pooyan , Rad Amir Hooshang Fallah , Heidarpour Mohammad , Azizzadeh Mohammad , Khoramian Babak 
Published: December 2019

Abstract
Background: This field trial was conducted to describe and compare the efficacy of two treatment regimens comprising of pre-calving systemic administrations of marbofloxacin and tylosin in combination with dry cow therapy. For more precise detection of Staphylococcus aureus IMI, PCR (nucA gene) and RAPD-PCR genotyping have been applied. A total of 841 quarters from 212 dry cows within two herds were assigned to three groups TYLO, MARB and CONT. At 21±3 days prior to parturition, tylosin was injected for three days (10 mg/kg, SC) to cows from TYLO and at the same time, single injection of marbofloxacin (8 mg/kg, SC) was administered to cows from MARB. Cows assigned to CONT served as an untreated control. All quarters allocated to the groups received intra-mammary infusion of Kanaclox DC® at drying off. Milk samples from all quarters at one week, before last milking of dry off, 3 and 7 DIM were obtained for bacteriological and oxidative analyses. Results: Despite the fact that no significant differences in total cure rate within the groups was demonstrated, the S. aureus cure rate achieved in TYLO and MARB were 74 and 73.5% respectively both of which being significantly higher than in CONT (58.1%). No significant differences in total new IMI were observed in TYLO (40.9%) and MARB (38.5%) groups compared with CONT group (42.5%). Furthermore the rate of new S. aureus IMI was higher in TYLO (24.5%) and MARB (24.1%) than in CONT (13.8%) group. Clinical mastitis rate in cows that received systemic injections (TYLO=3.8%, MARB=5.8%) was significantly lower than those detected in control cows (CONT=11.3%). Paired S. aureus isolates from dry off and post-calving have been clustered into 9 different RAPD types (A-I). 8 paired strains collected at dry off were identical to those at post-calving and 35 strains had more than 60% of dissimilarity. Conclusion: The study demonstrates that dealing with herds in which S. aureus IMIs are prevalent, using close up broad-spectrum systemic antimicrobial agents can be unrewarding for preventing new S. aureus IMIs. However close up administration of systemic antimicrobials have the potential to reduce the incidence of clinical mastitis during lactation.
All-trans retinoic acid reduces cancer stem cell-like cell-mediated resistance to gefitinib in NSCLC adenocarcinoma cells
Authors: Yao Wenxiu , Wang Liyang , Huang Huan , Li Xin , Wang Pinjia , Mi Kun , Cheng Jia , Liu Huifen , Gu Cuirong , Huang Lingxiao , Huang Jianming 
Published: December 2019

Abstract
Background: The enrichment of cancer stem cell-like cells (CSCs) has been considered to be responsible for tumor progression after an initial response to epidermal growth factor receptor (EGFR) tyrosine kinase inhibitors (EGFR-TKIs) in patients with non-small cell lung adenocarcinoma (NSCLC/ADC). All-trans retinoic acid (ATRA) has been shown to potentiate cancer chemotherapy due to its ability to induce signals for CSC differentiation. We therefore investigated whether ATRA could improve the response of NSCLC/ADC cells to TKI gefitinib. Methods: Treatment of NSCLC/ADC A549 and H1650 cells with gefitinib enriched the gefitinib surviving cells (GSCs). The expression of ALDH1A1 and CD44 and the IC50 values for gefitinib were determined by flow cytometry (FCM) and crystal violet assay in GSCs and ATRA-treated GSCs, respectively. Results: GSCs showed higher expression of ALDH1A1 and CD44 and IC50 values for gefitinib than their respective parental cells, suggesting that gefitinib can lead to propagation of CSC-enriched gefitinib-resistant cells. Treatment with ATRA was found to significantly reduce the increased expression of ALDH1A1 and CD44 and the IC50 values for gefitinib in A549GSC and H1650GSC cells. Conclusion: Our findings suggest that combination treatment with ATRA prevents gefitinib-induced enrichment of ALDH1A1bright/CD44high CSCs and enhances gefitinib-induced growth inhibition of NSCLC/ADC cells. Key words: All-trans retinoic acid, ALDH1A1, CD44, EGFR tyrosine kinase inhibitors, non-small cell lung adenocarcinoma
Efficient Synergistic Immunotherapy for Inhibiting Tumor Postoperative Recurrence and Metastasis via Calcium Alginate Hydrogel Nanosystem
Authors: Wang Tiange , Wang Hanjie , Zhang Yingying , He Tiandi , Wu Xiaoli , Chang Jin 
Published: December 2019

Abstract
Immunotherapy is expected to become an promising strategy in inhibiting tumor postoperative recurrence and metastasis. However, the effect is still unsatisfactory because of lacking cooperativity between various therapeutic methods. In this study, we designed an efficient synergistic immunotherapy system as an all-around and multi-dimension method for inhibiting tumor postoperative recurrence and metastasis. The efficient synergy lay in enhancing immune effect and eliminating immune suppression in the meantime by took advantages of CpG oligodeoxynucleotides (CpG ODNs) and antiPDL1 antibody. We introduced nanomaterials based on the calcium alginate hydrogel which has an acknowledged biological safety to implement the above strategy and make the system multifunctional. This nanosysterm have both therapy function and long-term monitoring capability in vivo to evaluate the recurrence and metastasis situation of postoperative residual tumor cells conveniently in real time. In vitro and vivo results proved that that this system could achieve a much better tumor inhibition efficiency and a good monitoring effect. This efficient synergistic immunotherapy nanosystem is expected to become a new promising strategy for postoperative immunotherapy of tumor.
Ethanol-activated CaMKII signaling induces neuronal apoptosis through Drp1-mediated excessive mitochondrial fission and JNK1-dependent NLRP3 inflammasome activation.
Authors: Lim Jae Ryong , Lee Hyun Jik , Jung Young Hyun , Kim Jun Sung , Chae Chang Woo , Kim Seo Yihl , Han Ho Jae 
Published: December 2019

Abstract
Background: Neurodegeneration is a representative phenotype of patients with chronic alcoholism. Ethanol-induced calcium overload causes NLRP3 inflammasome formation and an imbalance in mitochondrial dynamics, closely associated with the pathogenesis of neurodegeneration. However, how calcium regulates this process in neuronal cells is poorly understood. Therefore, the present study investigated the detailed mechanism of calcium-regulated mitochondrial dynamics and NLRP3 inflammasome formation in neuronal cells by ethanol. Methods: In this study, we used the SK-N-MC human neuroblastoma cell line. To confirm the expression level of the mRNA and protein, real time quantitative PCR and western blot were performed. Co-immunoprecipitation and Immunofluorescence staining were conducted to confirm the complex formation or interaction of the proteins. Flow cytometry was used to analyze intracellular calcium, mitochondrial dysfunction and neuronal apoptosis. Results: Ethanol increased cleaved caspase-3 levels and mitochondrial reactive oxygen species (ROS) generation associated with neuronal apoptosis. In addition, ethanol increased PKA activation and CREB phosphorylation, which increased NMDA receptor (NMDAR) expression. Ethanol-increased NMDAR induced intracellular calcium overload and CaMKII activation leading to phosphorylation of dynamin-related protein 1 (Drp1) and JNK1. Drp1 phosphorylation promoted Drp1 translocation to the mitochondria, resulting in excessive mitochondrial fission, mitochondrial ROS accumulation, and loss of mitochondrial membrane potential, which was recovered by Drp1 inhibitor pretreatment. Ethanol-induced JNK1 phosphorylation activated the NLRP3 inflammasome that induced caspase-1 dependent mitophagy inhibition, thereby exacerbating ROS accumulation and causing cell death. Suppressing caspase-1 induced mitophagy and reversed the ethanol-induced apoptosis in neuronal cells. Conclusions: Our results demonstrated that ethanol upregulated NMDAR-dependent CaMKII phosphorylation which is essential for Drp1-mediated excessive mitochondrial fission and the JNK1-induced NLRP3 inflammasome activation resulting in neuronal apoptosis.
A Phase II trial of Ofatumumab and Complement Replacement in Patients with Relapsed or Refractory Chronic Lymphocytic Leukemia
Authors: Tuscano Joseph M , Poh Christina , Rosenberg Aaron S , Jonas Brian A. , Barisone Gustavo , Lundeberg Kathleen , Schwab Emily , Kaesberg Paul 
Published: December 2019

Abstract
Background: While many humanized monoclonal antibodies utilize complement dependent cytotoxicity, the complement depleting effects of these antibodies and the effect of complement replacement are not well-described. This study sought to examine complement levels and the effect of complement repletion after treatment with ofatumumab in patients with chronic lymphocytic leukemia (CLL). Methods: Twelve patients with relapsed or refractory CLL were treated with ofatumumab in combination with fresh frozen plasma used as complement replacement. The primary endpoint was objective response rate. Correlative endpoints included complement levels C3 and C4 and complement activity. Results: Adverse events were minimal, and efficacy was encouraging with an overall response rate of 83% and 2 patients (17%) achieving a complete response. While only 2 (17%) of patients had low complement activity at baseline, 8 (67%) developed low levels of complement activity. At a median follow-up time of 37 months the median progression-free survival was 12.5 months. Conclusions: While a minority of patients had low complement activity at baseline, a majority developed low levels of complement with ofatumumab treatment. The magnitude of complement depletion did not correlate with response. Future trials are needed to further explore complement replacement as a less toxic strategy to improve efficacy of monoclonal antibody-based regimens in CLL.
Cellular Retinol Binding Protein I transfection in H460 non-small lung carcinoma cells reduces proliferation and AKT-related gene expression
Authors: Ferlosio Amedeo , Agostinelli Sara , Doldo Elena , Costanza Gaetana , Centofanti Federica , Orlandi Augusto 
Published: December 2019

Abstract
Background: In recent years, new treatments with novel action mechanisms have been explored for advancer lung cancer. Retinoids were shown to promote cancer cell differentiation and death of and their action is mediated from specific cytoplasmic and nuclear receptors. The purpose of this study was to investigate the effect of Cellular retinol binding Protein I (CRBPI) transfection in H460 human non-small cell lung cancer cell line normally not expressing that receptor.Methods: H460 cells were transfected by using a vector pTargeT Mammalian expression system carrying the whole sequence of CRBPI gene. For proliferation and apoptosis studies, cells were treated with different concentrations of all-trans retinoic acid ( at RA) and retinol. AKT-related gene expression was analyzed by using western blot and Signosis array. Results were analysed using d by one-way analysis of variance (ANOVA) followed from a Bonferroni post hoc test or with t-student test.Results: CRBPI + showed a reduced proliferation and viability in basal condition and after at RA treatment when compared to empty-transfected H460 cells. Reduced proliferation in CRBPI + H460 cells associated to the down-regulation of pAKT/pERK/pEGFR genes. In particular, gene array documented in CRBPI + H460 cells the down-regulation of AKT and Stat-3-related genes, including M-Tor, Akt1, Akt2, Akt3, Foxo1, Foxo3, p21, p27, pTen, Jun.Conclusion: Restoration of CRBPI expression in H460 cells reduced proliferation and viability in both basal condition and after at RA treatment, likely by down-regulating AKT-related gene level. Further studies are needed to better clarify how those CRBPI-related intracellular pathways contribute to counteract non-small cell lung carcinoma progression in order to suggest a potential tool to improve efficacy of retioid anti lung cancer adjuvant therapy.
A key genomic subtype associated with lymphovascular invasion in head and neck squamous cell carcinoma
Authors: Zhang Jian , Jiang Huali , Xie Tao , Wang Baiyao , Huang Xiaoting , Lin Jie , Xu Anan , Li Rong , Yuan Yawei 
Published: December 2019

Abstract
Objective: Lymphovascular invasion (LOI), a key pathological feature of head and neck squamous cell carcinoma (HNSCC), predicts poor survival. However, the associated clinical characteristics remain uncertain, and the molecular mechanisms are largely unknown. Methods: Weighted gene co-expression network analysis was performed to construct gene co-expression networks and investigate the relationship between modules and LOI clinical trait. Functional enrichment and KEGG pathway enrichment analysis were performed for differentially expressed genesusing DAVID database. The protein-protein interaction network was constructed using Cytoscape software, and module analysis was performed using MCODE. Survival analysis and unsupervised hierarchical clustering were used to evaluate the relationships among LOI-associated genomic subtype, clinicopathological features and patient outcomes. And the potential targeted LOI molecular agents were identiﬁed with DrugBank. Results: 10 co-expression modules in two key modules (turquoise and pink) associated with tumor LOI were identiﬁed. Functional enrichment and KEGG analysis identified turquoise and pink modules played signiﬁcant roles in the progression of HNSCC. The 24 genes in two modules were identiﬁed as hub genes. Clustering analysis with seven hub genes set further divided cases into subtypes 1 and 2, which were signiﬁcantly associated with pathology-determined LOI status in both cohorts. The 10-year overall survival of subtype 2 was signiﬁcantly worse than that of subtype 1. Conclusions: Our research revealed the key co-expression modules and identiﬁed seven prognostic biomarkers, including CCNA2, CNFN, DEPDC1, KIF18, KIF23, PRC1, TTK, which provide some new insights into LOI of HNSCC. Additionally, the small molecular agents may be a candidate drug for treating LOI.
Knowledge Of Glaucoma And Associated Factors Among Adults In Gish Abay Town, Northwest Ethiopia
Authors: Bizuneh Zewdu Yenegeta , Tsega Ayanaw , Addis Yezinash , Admassu Fisseha 
Published: December 2019

Abstract
Introduction Glaucoma is a global public health problem and it is the leading cause of irreversible blindness worldwide and the fifth cause of blindness in Ethiopia. Even though public knowledge of glaucoma is a key for early case identification and prevention of blindness, it is unknown in the study area, making provision of interventions difficult. Objective: To assess knowledge of glaucoma and associated factors among adults in Gish Abay town, Northwest Ethiopia, 2018 Methods: A Community based cross - sectional study design with systematic random sampling technique was used to select 630 adults. The study was conducted from April 25-May 05, 2018. Data was entered into Epi Info version 7 and was analysed by Statistical Package for Social Sciences version 23. Binary logistic regression model and adjusted odds ratio with 95% confidence level was used to identify the significant factors associated with knowledge of glaucoma. P–value ≤ 0.05 was considered statistically significant. Results: About 594 adults were participated with a response rate of 94%. Nearly 52% of the participants were females with median age of 28 years. The proportion of good knowledge was demonstrated in 68.9% [95% CI; 61, 75.9]. Educational status: secondary education [AOR; 4.01: 1.15, 13.94], college and above [AOR; 4.49: 1.31, 15.32], history of eye examination [AOR; 2.86: 1.05, 7.79] and younger age (18-22) [6.39: 1.82, 22.41] were positively associated with good knowledge of glaucoma. Conclusion and recommendation: The proportion of good knowledge of glaucoma was high. Better educational status, younger age, eye examination at least once in life and higher income level were associated with better knowledge of glaucoma. Recognizing the severity of glaucoma, enhancing knowledge is paramount at the community.
ANÁLISE NUMÉRICA DO EFEITO DA GEOMETRIA NA RESISTÊNCIA DE UM SISTEMA DE IMPLANTE DENTÁRIO
Authors: Felippi Christian , Da Silva Rafaela Lopes , De Almeida Julio Cézar 
Published: December 2019

Para garantir que, mesmo sob solicitações mecânicas repetitivas, não ocorram falhas em um sistema de implante dentário, é imprescindível compreender a influência dos parâmetros geométricos na resistência mecânica. Neste trabalho foi avaliado a influência da altura de transmucoso e do diâmetro de componentes protéticos retos na resistência mecânica em quatro grupos, considerando os extremos de cada parâmetro, em configuração de acordo com a norma ISO 14801. A avaliação se deu por simulações estáticas não lineares pelo método de elementos finitos, através do software comercial FEMAP. Os resultados mostraram que, de acordo com o que é apresentado em literatura, a porção entre a cervical e os três primeiros filetes da rosca externa do implante foi a que apresentou a maior solicitação mecânica e, portanto, a mais suscetível à falha. O diâmetro foi o parâmetro de maior influência, possuindo influência positiva (diminuição da tensão) com o aumento do mesmo. A altura de transmucoso teve um efeito positivo somente nos componentes de maior diâmetro e pouco impacto nos componentes protéticos de menor diâmetro. Concluiu-se ainda que o grupo com componente protético de menor diâmetro e maior altura foi o que obteve maiores tensões, sendo, portanto, o caso crítico para o ensaio mecânico.
ANÁLISE DE SENSIBILIDADE EM UM SISTEMA DE LEVITAÇÃO ACÚSTICA DE CAMPO PRÓXIMO SUJEITO A INCERTEZAS
Authors: Arruda Zuffi Geisa , Lobato Fran Sérgio , Lara – Molina Fabian Andres , Cavalini Junior Aldemir Aparecido , Steffen Junior Valder 
Published: December 2019

Este trabalho tem como objetivo analisar o efeito de parâmetros incertos em um sistema mecânico que representa o fenômeno da levitação acústica de campo próximo, cujo comportamento dinâmico é descrito pela equação de Reynolds. As incertezas consideradas são devidas às variações nas condições ambientais e no sistema de atuação, sendo estas representadas por variáveis aleatórias. As forças de sustentação associadas ao problema avaliado são obtidas a partir da solução do modelo e do método de simulação de Monte Carlo. Os resultados numéricos obtidos permitiram avaliar a resposta incerta do sistema e determinar a sensibilidade de cada parâmetro incerto na variação das forças de sustentação encontradas.
Epicardial Fat: A New Therapeutic Target in Psoriasis
Authors: Kamath Preetha , Benesh Gabrielle , Romanelli Paolo , Iacobellis Gianluca 
Published: December 2019

:
Psoriasis is a chronic inflammatory disease affecting over 8 million Americans. Importantly, patients
with psoriasis are at an increased risk of developing atherosclerosis, coronary artery disease, and myocardial
infarctions. Several studies have suggested that psoriasis may be an independent risk factor for cardiovascular
disease given their shared inflammatory properties and pathogenic similarities. Epicardial fat is also linked to
cardiovascular disease and may be an independent risk factor for atherosclerosis. It has been proposed that measuring
epicardial fat tissue may serve as a useful subclinical measure of cardiovascular disease in psoriasis patients.
Echocardiography has been increasingly adopted as an accurate, minimally invasive, and cost-effective
measure of determining the volume and thickness of epicardial fat. Using echocardiographic measures of epicardial
fat thickness as a marker of cardiovascular disease and therapeutic target in psoriasis patients may provide
clinicians with a means to better manage and hopefully prevent deleterious downstream effects.
Discovery of Substituted N-(6-Chloro-3-cyano-4-phenyl-4H-chromen-2-yl)- 2-(4-chloro-phenoxy)-acetamide for Biphasic Anticancer and Anticonvulsant Activities
Authors: Chauhan Divya , Hashim Syad Riaz , Rani Prinka , Kumar Sushil , Shrimal Navratan , Shastri Divya , Raj Vinit 
Published: December 2019

Background :Privileged 4H-chromenes possess potent anticancer and anticonvulsant activities. By the inspiring potency of 4H-chromenes and demands of the present era of scaffolds, an effective molecule was discovered for the treatment of cancer and related diseases.Objective :This study designed and synthesized a novel series of 4H-chromene derivatives from one-port synthesis for the treatment of cancer and other such diseases.Methods:A side amide chain was substituted in multiple steps on the amine group of chromene. Later, the anticancer activity of synthesized compounds was investigated against the human colon adenocarcinoma cell line (HT-29) using sulforhodamine B (SRB) assay. Moreover, anticonvulsant activity was also detected using maximal electroshock seizure (MES) model and subcutaneous Metrazol Seizure Threshold Test (scMET) in albino Wistar rats. Neurotoxicity was evaluated by using the rotarod test. Before the synthesis, docking studies were performed using various molecular targets. Subsequently, the computational study of the titled compounds was performed to predict the pharmacokinetic profile.Results:Among the fifteen tested compounds, A4 and A9 were found to be active against HT-29 cells (growth inhibitory dose 50% (GI50) <11μM). Moreover, compounds A4 showed the protection at 300mg/kg in scMET (h) for albino Wistar rats and compounds A9, A11, and A15 exhibited the anticonvulsant effect at the doses 100, 300 and 300 mg/kg, respectively in MES screen (h).Conclusion :Due to these encouraging results, we concluded that both A4 and A9 may be effective for treatement against colon cancer, while compound A9 may be used as a considerable effective molecule for the treatment of epilepsy.
A comparative study of Mawasangka and Wakatobi language in South East Sulawesi
Authors: Ana Haerun 
Published: December 2019

This research aims at investigating the relationship between Mawasangka language (ML) and Wakatobi language (WL) in Southeast Sulawesi. This research was conducted based on qualitative and quantitative methods (mixed methods). The qualitative analysis shows that both languages have similar vowel and consonants sounds, and phonetic long vocoid sounds. Besides, both languages have unique bilabial implosive consonant /ɓ/, alveolar implosive /ɗ/, velar fricative /ĝ/; and clusters of /ns/, /nt/, /nd/, /mb/, /ŋk/, /ŋg/, and /mp/; and all syllables are opened or categorized as vocalist languages. Both languages also have perfected phoneme correspondences. In addition, the result of the quantitative approach using the lexicostastics technique shows that the relationship of ML-WL is 43,43%. Based on the percentage, ML and WL show closed relationship which is categorized as different languages from the same language family.