bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of key genes and long non-coding RNA associated ceRNA networks in hepatocellular carcinoma
Authors: Liu Jun , Li Wenli , Zhang Jian , Ma Zhanzhong , Wu Xiaoyan , Tang Lirui 
Published: November 2019

Background
Hepatocellular carcinoma (HCC) is one of the leading causes of cancer-related deaths worldwide. Although multiple efforts have been made to understand the development of HCC, morbidity, and mortality rates remain high. In this study, we aimed to discover the mRNAs and long non-coding RNAs (lncRNAs) that contribute to the progression of HCC. We constructed a lncRNA-related competitive endogenous RNA (ceRNA) network to elucidate the molecular regulatory mechanism underlying HCC.

Methods
A microarray dataset (GSE54238) containing information about both mRNAs and lncRNAs was downloaded from the Gene Expression Omnibus database. Differentially expressed genes (DEGs) and lncRNAs (DElncRNAs) in tumor tissues and non-cancerous tissues were identified using the limma package of the R software. The miRNAs that are targeted by DElncRNAs were predicted using miRcode, while the target mRNAs of miRNAs were retrieved from miRDB, miRTarBas, and TargetScan. Functional annotation and pathway enrichment of DEGs were performed using the EnrichNet website. We constructed a protein–protein interaction (PPI) network of DEGs using STRING, and identified the hub genes using Cytoscape. Survival analysis of the hub genes and DElncRNAs was performed using the gene expression profiling interactive analysis database. The expression of molecules with prognostic values was validated on the UALCAN database. The hepatic expression of hub genes was examined using the Human Protein Atlas. The hub genes and DElncRNAs with prognostic values as well as the predictive miRNAs were selected to construct the ceRNA networks.

Results
We found that 10 hub genes (KPNA2, MCM7, CKS2, KIF23, HMGB2, ZWINT, E2F1, MCM4, H2AFX, and EZH2) and four lncRNAs (FAM182B, SNHG6, SNHG1, and SNHG3) with prognostic values were overexpressed in the hepatic tumor samples. We also constructed a network containing 10 lncRNA–miRNA–mRNA pathways, which might be responsible for regulating the biological mechanisms underlying HCC.

Conclusion
We found that the 10 significantly overexpressed hub genes and four lncRNAs were negatively correlated with the prognosis of HCC. Further, we suggest that lncRNA SNHG1 and the SNHG3-related ceRNAs can be potential research targets for exploring the molecular mechanisms of HCC.
Comparative Study for the Effect of Rigid and Semirigid Diaphragms on Reinforced Concrete Walls
Authors: Mustafa T. S. 
Published: November 2019

Rigid diaphragm is generally an acceptable option in most of the seismic design codes in which the in-plane deformability is not permitted because of the infinite in-plane stiffness properties. Several building configurations may exhibit significant flexibility in floor diaphragms and accordingly using the semirigid diaphragm is recommended as significant in-plane deformation does occur. In this study, a comparative study by using rigid and semirigid diaphragms is performed to identify the related effect on the reinforced concrete (RC) walls. In this study, a numerical study for a twelve-story building with dual system of RC columns and walls is performed. All geometrical and loading properties have been kept constant while using two types of diaphragm as rigid (RD) and semirigid (SRD). The seismic performance of the studied two structures was evaluated in terms of the fundamental period, maximum inter-story drift, maximum base shear and stresses on RC walls. Generally, RD produce results nearly identical to those of SRD for base shear, story displacements and inter-story drifts as the slab is sufficiently thick and membrane deformation due to lateral loading is negligible. Compared to SRD, using RD led to increase the internal moment and shear forces resulted from the seismic loads and acting on the RC walls while the resulting vertical loads are nearly identical.
Serum Vitamin D & Dry Eye Syndrome
Authors: . Shaveta , Nawaz Shah 
Published: November 2019

Introduction: Dry eye syndrome [DES] is one of the commonest ocular disorders characterized by tear film instability, visual discomfort & disturbance with ocular surface inflammation potentially leading to damage to the ocular surface. Serum Vitamin-D deficiency has been reported to be associated with DES.
Aim: To determine the Serum 25-hydroxy-vitamin-D levels in patients diagnosed as having DES.
Methods: We conducted a prospective observational study and included in our study, serum vitamin D levels of the first 40 patients over the age of 18 years presenting with signs and symptoms and later diagnosed to have DES [confirmed with ocular surface disease index-OSDI questionnaire, Schirmer I test, kerato-epitheliopathy examination and tear-film breakup time-[TBUT] were analysed.
Results: Schirmer's I test, interpreted as mild [11-15mm/5min], moderate [6-10 mm/5 min] and severe [<5mm/5min] had 8, 17 and 15 patients, respectively, with a mean value of 8 mm/5 min Schirmers I test. The minimum and maximum of Fluorescein TBUT measured in seconds was 4 and 10 seconds, respectively, with a mean of 6.2 seconds. Twenty four of our test subjects were found to have deficient Serum Vit 25[OH] D levels, nine had insufficient levels and seven had normal levels.
Conclusion: Vitamin D deficiency is prevalent in patient having DES.
BRINCADEIRAS INFANTIS NO ESTADO DE SÃO PAULO: UM ESTUDO DIATÓPICO E LÉXICO-SEMÂNTICO DE DENOMINAÇÕES PARA A “COBRA-CEGA” | CHILDREN ́S AMUSEMENT IN THE STATE OF SÃO PAULO: A DIATOPIC AND LEXICIAN-SEMANTIC STUDY OF DENOMINATIONS FOR COBRA-CEGA
Authors: Alencar Beatriz Aparecida , Isquerdo Aparecida Negri 
Published: November 2019

<div class="page" title="Page 1"><div class="section"><div class="layoutArea"><div class="column"><p><span>Resumo: </span><span>Este estudo analisa as denominações obtidas para a brincadeira em que uma criança, com os olhos vendados, tenta pegar as outras (COMITÊ NACIONAL DO PROJETO ALiB, 2001, p. 34), área semântica Jogos e Diversões Infantis. O universo da pesquisa compreendeu 188 inquéritos do Projeto ALiB realizados nas 37 localidades paulistas e 10 na área de controle, estas limítrofes à divisa estadual, com informantes selecionados segundo a metodologia do projeto ALiB. Para tanto, utilizaram-se os pressupostos da Dialetologia, da Lexicologia, da Semântica e da Etnolinguística, o que permitiu apurar que a variante cobra-cega foi a mais produtiva neste estudo, seguido por cabra-cega e que suas ocorrências se relacionam às características dos informantes e à história social das localidades em que foram documentadas.</span></p><div class="page" title="Page 2"><div class="layoutArea"><div class="column"><p><span>Abstract: </span><span>This study analyzes the denominations learned from a play in which a child is blindfolded and </span><span>tries to catch the others (COMITÊ NACIONAL..., 2001, p. 34), Semantic area Games and Children's </span><span>Amusement. The research universe is composed of 188 surveys from the ALiB Project conducted in 37 places of São Paulo and 10 of the control area, bordering the state border, with selected informants according to an ALIB methodology. In order to do so, the assumptions of Dialectic, Lexicology, Semantics and Ethnolinguistics were used, which helped to estabilish </span><span>the “cobra</span><span>-</span><span>cega” variant more productive in this study, followed for “cobra</span><span>-</span><span>cega” and that its occurrences are related to the characteristics of the </span><span>informants and the social history of the places where they were documented. </span></p><p><span>Keywords</span><span>: Diaclectology; ALiB Project; Games </span><span>and children’s amusement</span><span>; Cobra-cega; São Paulo. </span></p></div></div></div><p><span><br /></span></p></div></div></div></div>
O LÉXICO TOPONÍMICO DE ORIGEM AFRICANA NO RECÔNCAVO DA BAHIA: UMA ANÁLISE SEMÂNTICO-LEXICAL | THE TOPONYMIC LEXICON OF AFRICAN ORIGIN IN THE BAHIA RECONCAVO: A SEMANTIC-LEXICAL ANALYSIS
Authors: Santana Lana Crsitina , Paim Marcela 
Published: November 2019

<div class="page" title="Page 1"><div class="section"><div class="layoutArea"><div class="column"><p><span>Resumo: </span><span>Este artigo apresenta um estudo sobre a microtoponímia de origem africana dos vinte municípios que compõem o Território de Identidade do Recôncavo baiano (SEI, 2010), especificamente, os nomes dos povoados dessa região. Foram analisados 62 topônimos, os quais foram coletados dos mapas municipais estatísticos, escala 1:100.000, disponíveis na plataforma virtual do IBGE. O tratamento aplicado às informações obtidas seguem uma metodologia toponímica já existente nessa área, correspondente a um estudo etimológico dos termos que compõem o sintagma toponímico, buscando suas motivações semânticas </span><span>─ </span><span>característica principal desses signos </span><span>─ </span><span>encaixando-os em uma taxionomia desenvolvida por Dick (1990a, 1990b). </span></p><div class="page" title="Page 2"><div class="layoutArea"><div class="column"><p class="AbstractDL"><strong>Abstract:</strong> <em>This article presents a study on the microtoponymy of African origin of the twenty municipalities that compose the Identity Territory of the Bahian Recôncavo (SEI, 2010), specific, the names of village of the region. Were analyzed 62 toponyms, which were collected from the municipal statistical maps, scale 1: 100,000, available on the IBGE virtual platform. The treatment applied to the information obtained follows a toponymic methodology already existing in this area, corresponding to an etymological study of the terms that make up the toponymic phrase, seeking its semantic motivations </em><em>─</em><em> main characteristic of these signs </em><em>─</em><em> following from the insertion in a taxonomy developed by Dick (1990a, 1990b). </em></p><p class="AbstractDL"><strong>Keywords:</strong> <em>Toponymy; Sociocultural identity; Bahia Recôncavo.</em></p><p> </p></div></div></div></div></div></div></div>
EL ESPAÑOL DE LA PATAGONIA: UNA PROPUESTA DE ATLAS LINGÜÍSTICO DE TIERRA DEL FUEGO (ARG) | O ESPANHOL DA PATAGÔNIA: UMA PROPOSTA DE ATLAS LINGUÍSTICO DA TERRA DO FOGO (ARG)
Authors: Toranzos Romina , Kailer Dircel 
Published: November 2019

<p><strong>Resumen:</strong> Enmarcado en los estudios dialectológicos (ALVAR, 1996) nuestro trabajo expondrá aspectos del Atlas de pequeño dominio de Tierra del Fuego (Arg.). Sus objetivos son: i) revisar descripciones generales para el área de estudio, asociadas a la variedad de la Patagonia, ii) explorar factores extralingüísticos que puedan influir para una variedad con rasgos propios. Para ello, repasaremos los estudios dialectológicos y sociolingüísticos en Argentina y los aspectos sociohistóricos de la provincia, con fuertes procesos migratorios. Los resultados destacan, además de rasgos asociados al español de Chile, el contacto con otras variedades, lenguas indígenas y europeas, lo que nos llevará a pensar en las ventajas de adoptar los lineamientos de la Geolingüística Pluridimensional (THUN, 1996).</p><div class="page" title="Page 2"><div class="layoutArea"><div class="column"><p><span>Resumo: </span><span>Embasado em estudos dialetológicos (ALVAR, 1996), nosso trabalho expõe aspectos do Atlas de pequeno domínio da Terra do Fogo (Arg.). Seus objetivos são: i) revisar as descrições gerais para a área de estudo, associadas à variedade da Patagônia, ii) explorar fatores extralinguísticos que podem influenciar uma variedade com características próprias. Para tanto, revisamos estudos dialetológicos e sociolinguísticos na Argentina e os aspectos sócio-históricos da província, com fortes processos migratórios. Os resultados destacam, além das características associadas à variedade do Chile, o contato com outras variedades, línguas indígenas e europeias, o que nos leva a pensar nas vantagens de adotar os parâmetros da Geolinguística Pluridimensional (THUN, 1996). </span></p><p><span>Palavras-chave</span><span>: Atlas Linguístico; Terra do Fogo; Geolinguística Pluridimensional; Espanhol, Patagônia. </span></p></div></div></div>
A newly identified photolyase from Arthrospira platensis possesses a unique methenyltetrahydrofolate chromophore‐binding pattern
Authors: Yan Hui , Zhu Kongfu , Teng Maikun , Li Xu 
Published: November 2019

Cyclobutane pyrimidine dimers (CPD), as a common DNA damage caused by UV radiation, often lead to skin cancer. Here, we identified a photolyase from the alga Arthrospira platensis (designated as Ap‐phr), which has been regarded as a safe organism for humans for centuries, that can efficiently repair CPD lesions in ssDNA and dsDNA in vitro. The 1.6 Å resolution crystal structure of Ap‐phr revealed that it possesses a unique methenyltetrahydrofolate chromophore‐binding pattern with high energy transfer efficiency. Our study of Ap‐phr highlights its potential use in cosmetic, industrial and aesthetic medicine applications.
Calculating dose from a 2.5 MV imaging beam using a commercial treatment planning system
Authors: Ferris William S. , Culberson Wesley S. , Anderson Daniel R. , Labby Zacariah E. 
Published: November 2019

AbstractPatient dose from 2.5 MV images on the TrueBeam linear accelerator is not easily quantified, primarily because this beam energy is not normally modeled by commercial treatment planning systems. In this work we present the feasibility of using the Eclipse® treatment planning system to model this beam. The Acuros XB and the AAA dose calculation algorithms were tested. Profiles, PDDs, and output factors were measured for the 2.5 MV unflattened imaging beam and used for beam modeling. The algorithms were subsequently verified using MPPG 5.a guidelines. Calculated doses with both algorithms agreed with the measurement data to within the following criteria recommended for conventional therapeutic MV beams: 2% local dose‐difference in the high‐dose region, 3% global difference in the low‐dose region, 3 mm distance to agreement in the penumbra, and a gamma pass rate of >95% for 3%/3 mm criteria. Acuros was able to accurately calculate dose through cork and bone‐equivalent heterogeneities. AAA was able to accurately calculate dose through the bone‐equivalent heterogeneity but did not pass within the recommended criteria for the cork heterogeneity. For the 2.5 MV imaging beam, both the AAA and Acuros algorithms provide calculated doses that agree with measured results well within the 20% criteria for imaging beams recommended by AAPM TG‐180.
The Man in the Concrete Mask: The Metamorphosis of Charles‐Édouard Jeanneret
Authors: Glancey Jonathan 
Published: November 2019

AbstractHow did Le Corbusier make the transition from mountain boy to modernist master? How did he develop his architectural persona and his design identity, and how did he communicate it to maximum effect? British journalist, author and broadcaster Jonathan Glancey leads us through some of the critical moments in Le Corbusier's rise to architectural immortality.
Observations on Drawing: The Art of Architecture
Authors: Gallov Gabor 
Published: November 2019

AbstractHow important is the sketch and the hand drawing to the identity of the architect two decades into the 21st century? London‐based architect and architectural tutor Gabor Gallov argues it is still as crucial as it always was in conveying ideas, but equally as a vehicle for exploring the subtleties of individual architectural language away from the ubiquitous computer and rendering.
Test Bed: Communication Through Product Design
Authors: Howie Roger 
Published: November 2019

AbstractZaha Hadid Architects has become a global brand designing not only breathtaking buildings but also shoes, chairs, jewellery and tables, amongst other things. Roger Howie has been leading public relations for this maelstrom of creativity since 2004, and here describes how the practice applies its design ethos equally to these items as it does to architecture.
The Social Media Monster: Dangers and Thrills Only Partially Glimpsed
Authors: Furman Adam Nathaniel 
Published: November 2019

AbstractLondon‐based designer and architectural tutor Adam Nathaniel Furman has built a large number of followers on social media platforms such as Instagram. As well as using this to publish his work, awards, students’ work and the buildings he admires, he also uses it for more polemic effect, such as exposing those architects who have unpaid interns or attacking the politics of neoliberalism.
FOAM Impact: The Influence of Open‐access Medical Education on Practice Uptake
Authors: Pescatore Richard M. , Niforatos Joshua D. , Rezaie Salim R. , Swaminathan Anand , Morrison Mizuho , Reid Meaghan , Motov Sergey 
Published: November 2019

AbstractObjectivesThe FOAM Impact study sought to examine baseline rates of intravenous (IV) lidocaine usage for the treatment of renal colic and to compare rates of use between FOAM utilizers and nonutilizers. We sought to measure the effect of FOAM resources on clinical practice by timing the release of FOAM content with publication of the LIDOKET trial.MethodsA cross‐sectional before‐and‐after survey was conducted and disseminated on two social media platforms. The 13‐question, anonymous survey was posted for 1 week prior following the release of the LIDOKET study. Descriptive statistics, Mann‐Whitney t‐test, and chi‐square test statistics were used to describe survey respondent characteristics and Likert responses.ResultsThere were a total of 472 survey respondents. A total of 321 physicians (75.7% of total respondents) provided pre‐ and postpublication survey answers. There was no significant change in the use of analgesics before and after publication of LIDOKET and concurrent REBEL EM blog post. A total of 197 (42%) survey respondents reported using lidocaine for renal colic, of which 60 respondents (13%) reported frequent or occasional use. The mean difference in perceived efficacy of lidocaine before and after publication was −0.30 (95% confidence interval [CI] = −0.80 to 0.19, p = 0.15). Being a FOAM user was not associated with changes in mean difference in perception of lidocaine efficacy (F = 0.127, p = 0.72); however, there was a significant difference in perception of lidocaine’s efficacy following LIDOKET and REBEL EM publication (F = 4.718, p = 0.03).ConclusionsUsing an online survey‐based technique, no appreciable impact of FOAM resources was immediately apparent; however, engagement with FOAM was associated with a change in perception of IV lidocaine’s efficacy. To our knowledge, this is the first study of its kind to evaluate the impact of FOAM on clinical practice. The unique method of coordinating FOAM distribution with traditional medical publication may provide future opportunities for measuring the impact of asynchronous medical education resources on medical practice.
Synthesis of pyrrole‐bridged constrained geometry complexes and their application for olefin polymerization
Authors: Liu Jianfeng , Shao Lu , Feng Xiu , Xu Sheng , Mi Puke 
Published: November 2019

ABSTRACTIn this article, four constrained geometry complexes 4a [(η5‐C5H4)CH2(α‐C4H3N)]Ti(NMe2)2, 7a [(η5‐C9H6)CH2(α‐C4H3N)]Ti(NMe2)2, 4b [(η5‐C5H4)CH2(α‐C4H3N)]Zr(NEt2)2, 7b [(η5‐C9H6)CH2(α‐C4H3N)]Zr(NEt2)2 with pyrrole‐bridged fragment were synthesized and characterized by 1H NMR, 13C NMR, MASS, and EA. When combined with MAO, complex 4b given the highest activity, the activity of copolymerizing ethylene and 1‐hexene reached 2.48 × 106 g polymer/mol·M·h. The effects of temperature, pressure, and ratio of Al/Metal on the polymerization reaction and properties of polymers had been investigated. The polymers with these complexes were characterized by 13C NMR and DSC, and the results shown that polymer with 4a had the highest α‐olefin incorporation, the incorporation of 1‐hexene reached up to 9.81 mol%, and the 1‐octene was 8.84 mol%. Actually, there was no [HH] or [OO] sequence in the copolymer, according to the formula of reactivity ratio, rE • rH = 0, all these results suggested that the copolymerization mode is alternating copolymerization. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2020, 137, 48620.
The development of a population physiologically based pharmacokinetic model for mycophenolic mofetil and mycophenolic acid in humans using data from plasma, saliva, and kidney tissue
Authors: Alsmadi Mo'tasem M. , Alfarah Mahdi Qasem , Albderat Jawaher , Alsalaita Ghazi , AlMardini Reham , Hamadi Salim , Al‐Ghazawi Ahmad , Abu‐Duhair Omar , Idkaidek Nasir 
Published: November 2019

AbstractBackgroundMycophenolic acid (MPA) is used widely to prevent graft rejection in kidney‐transplant patients. Therapeutic drug monitoring (TDM) in plasma requires an invasive procedure that is inconvenient, especially in pediatric patients. TDM in saliva is a more convenient non‐invasive alternative compared with plasma.MethodsA population physiologically based pharmacokinetic (Pop‐PBPK) model of mycophenolate mofetil (MMF) and MPA with enterohepatic recycling was built and verified using previously published plasma, saliva, and kidney biopsy data in healthy and kidney‐transplant adult patients. The verified model was then used to predict experimentally observed plasma and saliva MMF and MPA TDM data in Jordanian pediatric kidney transplant patients measured using LC‐MS/MS. A correlation was established between plasma and saliva exposures in pediatrics.ResultsThe developed LCMS was sensitive to both MMF and MPA in plasma and saliva. The developed Pop‐PBPK model predicted well the previously reported MMF and MPA levels in plasma, saliva, and kidney tissue and those observed in the current study (more than 75% of observed data points were within 90% predictive interval of population simulations). A statistically significant correlation was found between plasma and saliva exposures for both MMF (Pop‐PBPK predicted and observed) and MPA (Pop‐PBPK predicted).ConclusionBoth MPA and MMF can be classified as class III compounds in the Salivary Excretion Classification System. Saliva is an alternative body fluid to plasma that can be used for TDM of MPA and MMF in kidney‐transplant patients in pediatrics. Exposure to MPA and MMF in plasma, saliva, and kidney tissue was reliably predicted using the developed Pop‐PBPK model.
Uncarboxylated osteocalcin promotes osteogenic differentiation of mouse bone marrow–derived mesenchymal stem cells by activating the Erk‐Smad/β‐catenin signalling pathways
Authors: Liu Zhongsheng , Yang Jianhong 
Published: November 2019

Uncarboxylated osteocalcin (unOc) is an osteoblast‐derived hormone with multiple regulatory functions. Osteocalcin knockdown delays the maturation of mineral species and downregulates the expression of osteogenic‐specific genes in human mesenchymal stromal cells. However, the underlying mechanisms remain unclear. Here, we investigated the effects of unOc on the osteogenic differentiation of mouse bone marrow–derived mesenchymal stem cells (BMSCs) and discovered that unOc promoted osteogenic differentiation of BMSCs, which was characterized by increases in alkaline phosphatase (ALP) activity, type I collagen (COLI) production, calcified nodule formation, and expression of osteogenic‐specific genes including the osterix, runt‐related transcription factor 2 (Runx2), ALP, and COLI genes. Further experiments indicated that unOc promoted the osteogenic differentiation of BMSCs via activation of the Erk‐Smad/β‐catenin signalling pathways.Significance of the studyOsteoporosis is associated with the osteogenic differentiation of BMSCs. In recent years, the role of unOc function as an endocrine hormone has received much attention. In this study, we reported for the first time that unOc promoted the osteogenic differentiation of mouse BMSCs through Erk‐Smad/β‐catenin signalling pathway. Our results highlight the importance of unOc as a hormone in promoting the osteogenic differentiation of BMSCs, indicating that this hormone may be beneficial in treatments for osteoporosis and fracture healing.