bluuebunny/crossref_metadata_2025_split huggingface dataset:
Laparoscopic Liver Resection: A South American Experience with 2887 Cases
Authors: Pekolj J. , Clariá Sánchez R. , Salceda J. , Maurette R. J. , Schelotto P. B. , Pierini L. , Cánepa E. , Moro M. , Stork G. , Resio N. , Neffa J. , Mc Cormack L. , Quiñonez E. , Raffin G. , Obeide L. , Fernández D. , Pfaffen G. , Salas C. , Linzey M. , Schmidt G. , Ruiz S. , Alvarez F. , Buffaliza J. , Maroni R. , Campi O. , Bertona C. , de Santibañes M. , Mazza O. , Belotto de Oliveira M. , Diniz A. L. , Enne de Oliveira M. , Machado M. A. , Kalil A. N. , Pinto R. D. , Rezende A. P. , Ramos E. J. B. , Talvane T. Oliveira A. , Torres O. J. M. , Jarufe Cassis N. , Buckel E. , Quevedo Torres R. , Chapochnick J. , Sanhueza Garcia M. , Muñoz C. , Castro G. , Losada H. , Vergara Suárez F. , Guevara O. , Dávila D. , Palacios O. , Jimenez A. , Poggi L. , Torres V. , Fonseca G. M. , Kruger J. A. P. , Coelho F. F. , Russo L. , Herman P. 
Published: June 2020

AbstractBackgroundLaparoscopic liver resections (LLR) have been increasingly performed in recent years. Most of the available evidence, however, comes from specialized centers in Asia, Europe and USA. Data from South America are limited and based on single‐center experiences. To date, no multicenter studies evaluated the results of LLR in South America. The aim of this study was to evaluate the experience and results with LLR in South American centers.MethodsFrom February to November 2019, a survey about LLR was conducted in 61 hepatobiliary centers in South America, composed by 20 questions concerning demographic characteristics, surgical data, and perioperative results.ResultsFifty‐one (83.6%) centers from seven different countries answered the survey. A total of 2887 LLR were performed, as follows: Argentina (928), Brazil (1326), Chile (322), Colombia (210), Paraguay (9), Peru (75), and Uruguay (8). The first program began in 1997; however, the majority (60.7%) started after 2010. The percentage of LLR over open resections was 28.4% (4.4–84%). Of the total, 76.5% were minor hepatectomies and 23.5% major, including 266 right hepatectomies and 343 left hepatectomies. The conversion rate was 9.7%, overall morbidity 13%, and mortality 0.7%.ConclusionsThis is the largest study assessing the dissemination and results of LLR in South America. It showed an increasing number of centers performing LLR with the promising perioperative results, aligned with other worldwide excellence centers.
Avowals and the project of inferentialism
Authors: Reichardt Bastian 
Published: June 2020

AbstractWhether there are philosophically relevant connections between the expressive role of first-personal vocabulary and self-knowledge is an on-going debate in analytical philosophy. We will take a look at this debate by considering Ludwig Wittgenstein’s distinction between the two uses of ‘I’ as object and as subject and work out a further distinction within the subject-use of ‘I’. This relates to a problem that is inherent in Robert Brandom’s inferentialist program regarding the role of first-personal vocabulary. It can be shown that subject-related aspects of language are necessary elements of inferentially articulated discourses—and not, like Brandom assumes, merely contingent features.
Refining Value Sensitive Design: A (Capability-Based) Procedural Ethics Approach to Technological Design for Well-Being
Authors: Cenci Alessandra , Cawthorne Dylan 
Published: June 2020

AbstractFundamental questions in value sensitive design include whether and how high-tech products/artefacts could embody values and ethical ideals, and how plural and incommensurable values of ethical and social importance could be chosen rationally and objectively at a collective level. By using a humanitarian cargo drone study as a starting point, this paper tackles the challenges that VSD’s lack of commitment to a specific ethical theory generates in practical applications. Besides, it highlights how mainstream ethical approaches usually related to VSD are incapable of solving main ethical dilemmas raised bytechnological design for well-beingin democratic settings. Accordingly, it is argued that VSD’s ethical-democratic import would substantially be enhanced by the espousal of aprocedural ethicsstance and thedeliberativeapproach to value and welfare entailed by Amartya Sen’s capability approach. Differently from rival ethical–political theories, its normative and meta-ethical foundations better handle human diversity, value-goal pluralism, conflicting vested interests as well as the epistemic-moral disagreements typical of contemporary complex democracies. Particularly, Sen’s capability approach procedural-deliberative tenets result in an “objective-impartial” choice procedure selecting a “hierarchy” of plural incommensurable values and rational goals thus, suitable to validate an applied science such as welfare-oriented technological design in concrete social environments. Conclusions suggest that refining VSD with a capability-based procedural approach to ethics fosters the concern for democracy and social justice while preserving vital scientific-technical standards. Major advantages are at an applied level to delivering ethically and socially justified, but yet highly functional technologies and high-tech products/artefacts.
Insulin und sein Rezeptor – Spezifität durch Kombinatorik?
Authors: Gutmann Theresia , Coskun ünal 
Published: June 2020

AbstractGlucose homeostasis and growth essentially depend on insulin engaging its receptor. Combining biochemistry, structural biology, and molecular dynamics simulations, we demonstrated how insulin binding stabilizes the insulin receptor in a T-shaped conformation with up to four bound ligands: two at receptor site 1 and - for the first time - two at site 2. These findings expand the current models of insulin binding to its receptor and potentially inform new approaches to structure-guided drug design.
Bakterien unter Stress - die stringent response
Authors: Steinchen Wieland , Bange Gert 
Published: June 2020

AbstractThe ability of microorganisms to cope with a large variety of environmental conditions is one of their most outstanding features. This review gives an overview over the metabolism and targets of ppGpp and pppGpp, the second messengers mediating resource allocation and adaptation to unfavourable conditions by triggering the ‘stringent response’.
Multiklonale Antikörper als Ersatz für Zweitantikörper aus Seren
Authors: Wenzel Esther Veronika , Russo Giulio , Dübel Stefan 
Published: June 2020

AbstractToday, recombinant antibodies can replace animal-derived primary antibodies in almost all applications. Due to their monoclonal origin and always known sequence, they offer optimal reproducibility. In contrast, almost all secondary antibodies are still made from animal sera. Multiclonal antibodies made by animal-free recombinant methods here offer a higher quality replacement for serum-derived secondary antibodies.
Grundlagen und Mechanismen der Neocortex-Expansion
Authors: Heide Michael , Huttner Wieland B. 
Published: June 2020

AbstractDuring primate, and notably human, evolution, the neocortex increased massively in size. This increase forms the basis of our higher cognitive abilities in comparison to other mammals. In recent years, major advances in understanding this evolutionary expansion have been achieved. Here, we would like to discuss the genetic foundation, principles and mechanisms underlying neocortical expansion.
Visualisierung von Mutationen auf Einzelzellebene
Authors: Täuber Sarah , Dormeyer Miriam , Commichau Fabian M. , Grünberger Alexander 
Published: June 2020

AbstractBacterial mutations have been investigated since many years, but they remain difficult to observe directly in single cells, which limits the analysis of the underlying molecular mechanism. However, for the investigation of mutations at the level of single cells, precise analytical tools are currently developed. This article describes a workflow for visualizing mutations in single cells and lays the foundation for the quantification of bacterial mutation rates in the future.
The Cycladic Blueschist Unit on Tinos, Greece: Cold NE Subduction and SW Directed Extrusion of the Cycladic Continental Margin Under the Tsiknias Ophiolite
Authors: Lamont Thomas N. , Searle Michael P. , Gopon Phillip , Roberts Nick M. W. , Wade Jon , Palin Richard M. , Waters David J. 
Published: June 2020

AbstractHigh pressure‐low temperature (HP‐LT) metamorphic rocks structurally beneath the Tsiknias Ophiolite make up the interior of Tinos Island, Greece, but their relationship with the overlying ophiolite is poorly understood. Here, new field observations are integrated with petrological modeling of eclogite and blueschists to provide new insight into their tectonothermal evolution. Pseudomorphed lawsonite‐, garnet‐, and glaucophane‐bearing schists exposed at the highest structural levels of Tinos (Kionnia and Pyrgos Subunits) reached ~22–26 kbar and 490–520°C under water‐saturated conditions, whereas pseudomorphed lawsonite‐ and aegirine‐omphacite bearing eclogite reached ~20–23 kbar and 530–570°C. These rocks are separated from rocks at deeper structural levels (Sostis Subunit) by a top‐to‐SW thrust. The Sostis Subunit records P‐T conditions of ~18.5 kbar and 480–510°C and is overprinted by pervasive top‐to‐NE shearing that developed during exhumation from (M1) blueschist to (M2) greenschist facies conditions of ~7.3 ± 0.7 kbar and 536 ± 16°C. These P‐T‐D relationships suggest that the Cycladic Blueschist Unit represents a discrete series of tectonometamorphic subunits that each experienced different tectonic and thermal histories. These subunits were buried to variable depths and sequentially extruded toward the SW from a NE dipping subduction zone. The difference in age and P‐T conditions between the HP‐LT rocks and the overlying metamorphic sole of the Tsiknias Ophiolite suggests that this NE dipping subduction zone was active between circa 74 and 46 Ma and cooled at a minimum rate of ~1.2–1.5°C/km/Myr prior to continent‐continent collision between Eurasia and Adria/Cyclades.
Autophagy as a decisive process for cell death
Authors: Jung Seonghee , Jeong Hyeonjeong , Yu Seong-Woon 
Published: June 2020

AbstractAutophagy is an intracellular catabolic pathway in which cellular constituents are engulfed by autophagosomes and degraded upon autophagosome fusion with lysosomes. Autophagy serves as a major cytoprotective process by maintaining cellular homeostasis and recycling cytoplasmic contents. However, emerging evidence suggests that autophagy is a primary mechanism of cell death (autophagic cell death, ACD) and implicates ACD in several aspects of mammalian physiology, including tumor suppression and psychological disorders. However, little is known about the physiological roles and molecular mechanisms of ACD. In this review, we document examples of ACD and discuss recent progress in our understanding of its molecular mechanisms.
Identification of the SRC-family tyrosine kinase HCK as a therapeutic target in mantle cell lymphoma
Authors: Lantermans Hildo C. , Minderman Marthe , Kuil Annemieke , Kersten Marie-José , Pals Steven T. , Spaargaren Marcel 
Published: June 2020

AbstractMantle cell lymphoma (MCL) is an aggressive non-Hodgkin lymphoma subtype arising from naïve B cells. Although novel therapeutics have improved patient prognosis, drug resistance remains a key problem. Here, we show that the SRC-family tyrosine kinase hematopoietic cell kinase (HCK), which is primarily expressed in the hematopoietic lineage but not in mature B cells, is aberrantly expressed in MCL, and that high expression of HCK is associated with inferior prognosis of MCL patients. HCK expression is controlled by the toll-like receptor (TLR) adaptor protein MYD88 and can be enhanced by TLR agonists in MCL cell lines and primary MCL. In line with this, primary MCL with high HCK expression are enriched for a TLR-signaling pathway gene set. Silencing of HCK expression results in cell cycle arrest and apoptosis. Furthermore, HCK controls integrin-mediated adhesion of MCL cells to extracellular matrix and stromal cells. Taken together, our data indicate that TLR/MYD88-controlled aberrant expression of HCK plays a critical role in MCL proliferation and survival as well as in retention of the malignant cells in the growth- and survival-supporting lymphoid organ microenvironment, thereby contributing to lymphomagenesis. These novel insights provide a strong rationale for therapeutic targeting of HCK in MCL.
Amyloid-beta modulates the association between neurofilament light chain and brain atrophy in Alzheimer’s disease
Authors: Kang Min Su , Aliaga Arturo Aliaga , Shin Monica , Mathotaarachchi Sulantha , Benedet Andrea L. , Pascoal Tharick A. , Therriault Joseph , Chamoun Mira , Savard Melissa , Devenyi Gabriel A. , Mathieu Axel , Chakravarty M. Mallar , Sandelius Åsa , Blennow Kaj , Zetterberg Henrik , Soucy Jean-Paul , Cuello A. Claudio , Massarweh Gassan , Gauthier Serge , Rosa-Neto Pedro ,   Alzheimer’s Disease Neuroimaging Initiative
Published: June 2020

AbstractNeurofilament light chain (NFL) measurement has been gaining strong support as a clinically useful neuronal injury biomarker for various neurodegenerative conditions. However, in Alzheimer’s disease (AD), its reflection on regional neuronal injury in the context of amyloid pathology remains unclear. This study included 83 cognitively normal (CN), 160 mild cognitive impairment (MCI), and 73 AD subjects who were further classified based on amyloid-beta (Aβ) status as positive or negative (Aβ+ vs Aβ−). In addition, 13 rats (5 wild type and 8 McGill-R-Thy1-APP transgenic (Tg)) were examined. In the clinical study, reduced precuneus/posterior cingulate cortex and hippocampal grey matter density were significantly associated with increased NFL concentrations in cerebrospinal fluid (CSF) or plasma in MCI Aβ+ and AD Aβ+. Moreover, AD Aβ+ showed a significant association between the reduced grey matter density in the AD-vulnerable regions and increased NFL concentrations in CSF or plasma. Congruently, Tg rats recapitulated and validated the association between CSF NFL and grey matter density in the parietotemporal cortex, entorhinal cortex, and hippocampus in the presence of amyloid pathology. In conclusion, reduced grey matter density and elevated NFL concentrations in CSF and plasma are associated in AD-vulnerable regions in the presence of amyloid positivity in the AD clinical spectrum and amyloid Tg rat model. These findings further support the NFL as a neuronal injury biomarker in the research framework of AD biomarker classification and for the evaluation of therapeutic efficacy in clinical trials.
Antibiotic prophylaxis for implant placement: a systematic review of effects on reduction of implant failure
Authors: Kim Andy , Abdelhay Nancy , Levin Liran , Walters John D. , Gibson Monica P. 
Published: June 2020

AbstractIntroduction Despite excellent reviews in the past several years, the use of antibiotics as prophylaxis for implant placement remains controversial.Aim To assess the literature on the efficacy of prophylactic antibiotics prescribed prior to and immediately following implant surgery (PIFS).Outcomes Whether administration of antibiotics reduced implant failure and post-operative complications.Design Databases searched were PubMed and Medline via Ovid (1946 to February 2018), Cochrane Library (Wiley) and Google Scholar.Materials and methods Quality assessment, meta-analysis with a forest plot and incorporated assessment of heterogeneity. A two-tailed paired t-test was performed, analysing differences in mean failure rates between groups.Results Fourteen publications were collected; 5,334 implants were placed with pre-operative antibiotics, 82 implants with antibiotics PIFS and 3,862 placed with no antibiotics. The overall risk ratio (RR) was 0.47 (95% CI 0.39-0.58), with the implant failure rates significantly affected by pre-operative intervention (Z = 7.00, P <0.00001). The number needed to treat (NNT) was 35 (95% CI 26.3-48.2). The difference between mean failure rates was statistically significant (P = 0.0335).Conclusion Administering prophylactic antibiotics reduced the risk of implant failures. Further investigations are recommended to establish a standardised protocol for the proper use of antibiotic regimen.
Large-scale metabolic interaction network of the mouse and human gut microbiota
Authors: Lim Roktaek , Cabatbat Josephine Jill T. , Martin Thomas L. P. , Kim Haneul , Kim Seunghyeon , Sung Jaeyun , Ghim Cheol-Min , Kim Pan-Jun 
Published: June 2020

AbstractThe role of our gut microbiota in health and disease is largely attributed to the collective metabolic activities of the inhabitant microbes. A system-level framework of the microbial community structure, mediated through metabolite transport, would provide important insights into the complex microbe-microbe and host-microbe chemical interactions. This framework, if adaptable to both mouse and human systems, would be useful for mechanistic interpretations of the vast amounts of experimental data from gut microbiomes in murine animal models, whether humanized or not. Here, we constructed a literature-curated, interspecies network of the mammalian gut microbiota for mouse and human hosts, called NJC19. This network is an extensive data resource, encompassing 838 microbial species (766 bacteria, 53 archaea, and 19 eukaryotes) and 6 host cell types, interacting through 8,224 small-molecule transport and macromolecule degradation events. Moreover, we compiled 912 negative associations between organisms and metabolic compounds that are not transportable or degradable by those organisms. Our network may facilitate experimental and computational endeavors for the mechanistic investigations of host-associated microbial communities.
CONY: A Bayesian procedure for detecting copy number variations from sequencing read depths
Authors: Wei Yu-Chung , Huang Guan-Hua 
Published: June 2020

AbstractCopy number variations (CNVs) are genomic structural mutations consisting of abnormal numbers of fragment copies. Next-generation sequencing of read-depth signals mirrors these variants. Some tools used to predict CNVs by depth have been published, but most of these tools can be applied to only a specific data type due to modeling limitations. We develop a tool for copy number variation detection by a Bayesian procedure, i.e., CONY, that adopts a Bayesian hierarchical model and an efficient reversible-jump Markov chain Monte Carlo inference algorithm for whole genome sequencing of read-depth data. CONY can be applied not only to individual samples for estimating the absolute number of copies but also to case-control pairs for detecting patient-specific variations. We evaluate the performance of CONY and compare CONY with competing approaches through simulations and by using experimental data from the 1000 Genomes Project. CONY outperforms the other methods in terms of accuracy in both single-sample and paired-samples analyses. In addition, CONY performs well regardless of whether the data coverage is high or low. CONY is useful for detecting both absolute and relative CNVs from read-depth data sequences. The package is available at https://github.com/weiyuchung/CONY.
Noise-induced switching from a symmetry-protected shallow metastable state
Authors: Tadokoro Yukihiro , Tanaka Hiroya , Dykman M. I. 
Published: June 2020

AbstractWe consider escape from a metastable state of a nonlinear oscillator driven close to triple its eigenfrequency. The oscillator can have three stable states of period-3 vibrations and a zero-amplitude state. Because of the symmetry of period-tripling, the zero-amplitude state remains stable as the driving increases. However, it becomes shallow in the sense that the rate of escape from this state exponentially increases, while the system still lacks detailed balance. We find the escape rate and show how it scales with the parameters of the oscillator and the driving. The results facilitate using nanomechanical, Josephson-junction based, and other mesoscopic vibrational systems for studying, in a well-controlled setting, the rates of rare events in systems lacking detailed balance. They also describe how fluctuations spontaneously break the time-translation symmetry of a driven oscillator.
The seed water content as a time-independent physiological trait during germination in wild tree species such as Ceiba aesculifolia
Authors: Gómez-Maqueo Ximena , Soriano Diana , Velázquez-Rosas Noé , Alvarado-López Sandra , Jiménez-Durán Karina , Garciadiego María del Mar , Gamboa-deBuen Alicia 
Published: June 2020

AbstractSeeds constitute a key physiological stage in plants life cycle. During seed germination, there is a spatial-temporal imbibition pattern that correlates with described physiological processes. However, only the moment of testa rupture has been described as a critical, discrete stage. Could a specific relative water content (RWC) value reflect a physiological stage useful for comparisons between seed batches? We tracked seed-by-seed imbibition during germination to homogenize sampling and selected a transcriptomic approach to analyse the physiological transitions that occur in seed batches collected in different years and with contrasting phenotypic responses to a priming treatment. The seed RWC reflected the transcriptional transitions that occur during germination, regardless of imbibition time or collection year, and revealed a set of biological processes that occur in the dry seed and during early germination are associated with the phenotypic response to priming. As climate shifts, so do the timing of developmental events important for determining organismal fitness, and poses another challenge to the comprehension of molecular and physiological processes driving the interaction between organisms and environment. In this study, we demonstrate that the use of physiological traits, specific to a particular developmental stage, is a reliable time-independent approach.
Substrate Channeling via a Transient Protein-Protein Complex: The case of D-Glyceraldehyde-3-Phosphate Dehydrogenase and L-Lactate Dehydrogenase
Authors: Svedružić Željko M. , Odorčić Ivica , Chang Christopher H. , Svedružić Draženka 
Published: June 2020

AbstractSubstrate channeling studies have frequently failed to provide conclusive results due to poor understanding of this subtle phenomenon. We analyzed the mechanism of NADH-channeling from D-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) to L-lactate Dehydrogenase (LDH) using enzymes from different cells. Enzyme kinetics studies showed that LDH activity with free NADH and GAPDH-NADH complex always take place in parallel. The channeling is observed only in assays that mimic cytosolic conditions where free NADH concentration is negligible and the GAPDH-NADH complex is dominant. Molecular dynamics and protein-protein interaction studies showed that LDH and GAPDH can form a leaky channeling complex only at the limiting NADH concentrations. Surface calculations showed that positive electric field between the NAD(H) binding sites on LDH and GAPDH tetramers can merge in the LDH-GAPDH complex. NAD(H)-channeling within the LDH-GAPDH complex can be an extension of NAD(H)-channeling within each tetramer. In the case of a transient LDH-(GAPDH-NADH) complex, the relative contribution from the channeled and the diffusive paths depends on the overlap between the off-rates for the LDH-(GAPDH-NADH) complex and the GAPDH-NADH complex. Molecular evolution or metabolic engineering protocols can exploit substrate channeling for metabolic flux control by fine-tuning substrate-binding affinity for the key enzymes in the competing reaction paths.