bluuebunny/crossref_metadata_2025_split huggingface dataset:
Does co-expression ofYarrowia lipolyticagenes encoding Yas1p, Yas2p and Yas3p make a potential alkane-responsive biosensor inSaccharomyces cerevisiae?
Authors: Dabirian Yasaman , Skrekas Christos , David Florian , Siewers Verena 
Published: September 2020

ABSTRACTAlkane-based biofuels are desirable to produce at a commercial scale as these have properties similar to our current petroleum-derived transportation fuels. Rationally engineering microorganisms to produce a desirable compound, such as alkanes, is, however, challenging. Metabolic engineers are therefore increasingly implementing evolutionary engineering approaches combined with high-throughput screening tools, including metabolite biosensors, to identify productive targets. EngineeringSaccharomyces cerevisiaeto produce alkanes can be facilitated by using an alkane-responsive biosensor, which can potentially be developed from the native alkane-sensing system inYarrowia lipolytica, a well-known alkane-assimilating yeast. This putative alkane-sensing system is, at least, based on three different transcription factors (TFs) named Yas1p, Yas2p and Yas3p. Although this system is not fully elucidated inY. lipolytica, we were interested in evaluating the possibility of translating this system into an alkane-responsive biosensor inS. cerevisiae. We evaluated the alkane-sensing system inS. cerevisiaeby developing one sensor based on the nativeY. lipolytica ALK1promoter and one sensor based on the nativeS. cerevisiae CYC1promoter. In both systems, we found that the TFs Yas1p, Yas2p and Yas3p do not seem to act in the same way as these have been reported to do in their native host. Additional analysis of the TFs suggests that more knowledge regarding their mechanism is needed before a potential alkane-responsive sensor based on theY. lipolyticasystem can be established inS. cerevisiae.
Selection of lamb size and early pregnancy in Soay sheep (Ovies aries)
Authors: Janeiro Maria João , Henshaw Jonathan M. , Pemberton Josephine M. , Pilkington Jill G. , Morrissey Michael B. 
Published: September 2020

AbstractThe paradox of stasis – the unexpectedly slow evolution of heritable traits under direct selection – has been widely documented in the last few decades. This paradox is often particularly acute for body size, which is often heritable and where positive associations of size and fitness are frequently identified, but constraints to the evolution of larger body sizes are often not obvious. Here, we identify a trade-off between survival and size-dependent reproduction in Soay sheep (Ovis aries), contributes to selection against large body size. Using recently developed theory on non-linear developmental systems, then decompose total selection of ewe lamb mass along different causal paths to fitness. Larger lambs are more likely to become pregnant, which has a large viability cost. After controlling for this pathway, however, the association between lamb mass and subsequent lifetime fitness is positive. Thus this trade-off does not fully explain stasis of size in tis population, but it does substantially reduce the strength of positive directional selection of size that would otherwise occur. While selection currently favours reduced probability of early pregnancy, largely irrespective of body size, it is likely that the occurrence of early pregnancy could result from adaptation to conditions during a recent period during which population density was much lower.
Rhythmic expression ofNeurofibromin 1in mushroom body neurons mediates circadian wake drive through activating cAMP−PKA signaling
Authors: Almeida Pedro Machado , Solis Blanca Lago , Feidler Alexis , Nagoshi Emi 
Published: September 2020

SUMMARYVarious behavioral and cognitive states exhibit circadian variations in animals across phyla includingDrosophila, in which only ∼0.1% of the entire brain neurons contain circadian clocks. This suggests that clock neurons communicate with a plethora of non-clock neurons to transmit the timing information to gate various behavioral outputs inDrosophila. Here, we address the molecular underpinning of this phenomenon by performing circadian RNA-seq analysis of non-clock neurons that constitute the mushroom body (MB), the center of information processing and sleep regulation. We identify hundreds of genes rhythmically expressed in the MB, including theDrosophilaortholog ofNeurofibromin 1(Nf1), the gene responsible for neurofibromatosis type 1 (NF1). Rhythmic expression ofNf1promotes daytime wakefulness by activating cAMP−PKA signaling and increasing excitability of the MB. These findings reveal the pervasive, non-cell-autonomous circadian regulation of gene expression in the brain and its role in sleep, with implications in the pathology of NF1.
Transcriptional activity differentiates families of Marine Group IIEuryarchaeotain the coastal ocean
Authors: Damashek Julian , Okotie-Oyekan Aimee Oyinlade , Gifford Scott Michael , Vorobev Alexey , Moran Mary Ann , Hollibaugh James Timothy 
Published: September 2020

ABSTRACTMarine Group IIEuryarchaeota(CandidatusPoseidoniales), abundant but yet-uncultivated members of marine microbial communities, are thought to be (photo)heterotrophs that metabolize dissolved organic matter (DOM) such as lipids and peptides. However, little is known about their transcriptional activity. We mapped reads from a metatranscriptomic time series collected at Sapelo Island (GA, USA) to metagenome-assembled genomes to determine the diversity of transcriptionally-activeCa. Poseidoniales. Summer metatranscriptomes had the highest abundance ofCa. Poseidoniales transcripts, mostly from the O1 and O3 genera withinCa. Thalassarchaeaceae (MGIIb). In contrast, transcripts from fall and winter samples were predominantly fromCa. Poseidoniaceae (MGIIa). Genes encoding proteorhodopsin, membrane-bound pyrophosphatase, peptidase/proteases, and part of the β-oxidation pathway were highly transcribed across abundant genera. Highly transcribed genes specific toCa. Thalassarchaeaceae included xanthine/uracil permease and receptors for amino acid transporters. Enrichment ofCa. Thalassarchaeaceae transcript reads related to protein/peptide, nucleic acid, and amino acid transport and metabolism, as well as transcript depletion during dark incubations, provided further evidence of heterotrophic metabolism. Quantitative PCR analysis of South Atlantic Bight samples indicated consistently abundantCa. Poseidoniales in nearshore and inshore waters. Together, our data suggestCa. Thalassarchaeaceae are important photoheterotrophs potentially linking DOM and nitrogen cycling in coastal waters.
Positive selection within the genomes of SARS-CoV-2 and other Coronaviruses independent of impact on protein function
Authors: Berrio Alejandro , Gartner Valerie , Wray Gregory A 
Published: September 2020

AbstractBackgroundThe emergence of a novel coronavirus (SARS-CoV-2) associated with severe acute respiratory disease (COVID-19) has prompted efforts to understand the genetic basis for its unique characteristics and its jump from non-primate hosts to humans. Tests for positive selection can identify apparently nonrandom patterns of mutation accumulation within genomes, highlighting regions where molecular function may have changed during the origin of a species. Several recent studies of the SARS-CoV-2 genome have identified signals of conservation and positive selection within the gene encoding Spike protein based on the ratio of synonymous to nonsynonymous substitution. Such tests cannot, however, detect changes in the function of RNA molecules.MethodsHere we apply a test for branch-specific oversubstitution of mutations within narrow windows of the genome without reference to the genetic code.ResultsWe recapitulate the finding that the gene encoding Spike protein has been a target of both purifying and positive selection. In addition, we find other likely targets of positive selection within the genome of SARS-CoV-2, specifically within the genes encoding Nsp4 and Nsp16. Homology-directed modeling indicates no change in either Nsp4 or Nsp16 protein structure relative to the most recent common ancestor. Thermodynamic modeling of RNA stability and structure, however, indicates that RNA secondary structure within both genes in the SARS-CoV-2 genome differs from those of RaTG13, the reconstructed common ancestor, and Pan-CoV-GD (Guangdong). These SARS-CoV-2-specific mutations may affect molecular processes mediated by the positive or negative RNA molecules, including transcription, translation, RNA stability, and evasion of the host innate immune system. Our results highlight the importance of considering mutations in viral genomes not only from the perspective of their impact on protein structure, but also how they may impact other molecular processes critical to the viral life cycle.
Online food delivery services and behavioural intention – a test of an integrated TAM and TPB framework
Authors: Troise Ciro , O'Driscoll Aidan , Tani Mario , Prisco Anna 
Published: September 2020

PurposeThis research leverages an integrated framework that uses the technology acceptance model (TAM) and the theory of planned behaviour (TPB) to analyse the main drivers of users' intention to use food delivery apps. The purpose of this paper is to investigate the consumer's willingness to adopt online food delivery (OFD) using the models' constructs and extend them to consider food choices, convenience, trust and the effect of the perceived risks related to the coronavirus disease 2019 (COVID-19) pandemic as contextual factors.Design/methodology/approachThe study adopts the partial least squares approach to structural equation modelling (PLS-SEM) to examine the data. The final sample consists of 425 people in Italy.FindingsThe authors have found that combining the TAM and the TPB provides a valid and significant model that can be used to understand OFD users' behavioural intentions. Moreover, the results show that subjective norms have a stronger effect on behavioural intentions than the personal attitude and that trustworthiness and the perception of risks related to COVID-19 have different effects. Accordingly, the authors derive several theoretical and managerial implications from these results.Originality/valueThis research contributes to the current debate on consumer behaviour in the OFD context. Only a few studies have integrated the TAM and TPB models in this context. This paper sheds light on the factors useful in predicting people's choice to buy food via OFD. Furthermore, it highlights the key role of some contextual factors and subjective norms over more technical ones.
Evaluating renewable energy choices among rural communities in Nigeria. An insight for energy policy
Authors: Okwanya Innocent , Alhassan Abdulkareem , Migap Job Pristine , Adeka Sunday Simeon 
Published: September 2020

Purpose
This study aims to examine the effects of policy incentives and cost on the choice and use of renewable energy in North-Central Nigeria.

Design/methodology/approach
The data for this study are collected from a sample of 290 respondents drawn from across 6 states in North-Central Nigeria, including the Federal Capital Territory. This study uses descriptive statistics and multinomial logistic regression to analyze the data.

Findings
The findings reveal that there is a huge potential demand for renewable energy sources (particularly solar photovoltaic) in the rural communities in Nigeria. It also indicates a positive and highly significant relationship between the level of awareness, availability and income and the use (consumption) of renewable energy sources among the rural communities. Furthermore, the cost of installation and maintenance of renewable energy, its reliability and availability are significant determinants of renewable energy choices among rural inhabitants in Nigeria.

Practical implications
The authors submit that inefficient policy strategies, high cost of installation and lack of awareness remain the major hindrances to the use of more efficient renewable energy sources. From a policy point of view, a viable strategy for effective use of renewable energy sources is the involvement of government, development partners and agencies for the funding of renewable energy technology in the rural sector of the country. The usage of modern renewable energy would increase if policy incentives are aimed at covering parts of the maintenance and installation cost of renewable energy users. The authors recommend that apart from creating awareness on the benefits of renewable energy, policymakers should provide a desirable policy environment for private energy firms to supply renewable energy at an affordable cost to the rural communities in Nigeria.

Social implications
A majority of the rural households in Nigeria, as shown in this article, are poor and therefore use firewood as their main source of cooking energy because of the cost of renewable energy.

Originality/value
Despite the abundance of renewable energy sources and government effort at improving renewable energy use, more than 15 million people live without access to electricity and 54 million are without modern energy services for cooking and lightening in Nigeria. A total of 61% of these people live in rural areas. Therefore, this study is novel in providing energy policy insights for rural communities in North-Central Nigeria.
Using photovoice to explore the impact on a student community after including cross-sectional content on environmental sustainability in a university subject: a case study
Authors: Coronado Carmen , Freijomil-Vázquez Carla , Fernández-Basanta Sara , Andina-Díaz Elena , Movilla-Fernández María-Jesús 
Published: September 2020

PurposeHigher education institutions have a significant impact in preparing future generations for the creation of a sustainable society. By formulating appropriate curricula, the university can shape student personality with sustainability concerns. This study aims to present the results of a teaching approach on environmental sustainability using the photovoice methodology. A guided visit to the sewage treatment plant of A Coruña was included as a teaching activity in the “Microbiology and Parasitology” classes of the podiatry degree at University of A Coruña. The teaching objectives were to reinforce contents through observation and to introduce citizen awareness on sustainability and responsible water use in a cross-sectional manner.Design/methodology/approachIn this case study, different steps of photovoice as a qualitative participatory action methodology were developed. A total of 43 university students willingly participated with their photographs. Qualitative data were collected from the students’ photovoice visit reports and a subsequent discussion group. Thematic content analysis was performed manually.FindingsThis study explored the impact of an environmental sustainability teaching activity on the university student community. Six main categories emerged from the qualitative analysis: savings/waste of water, misuse of the water closet, disposing of used oil, solid waste/trash, reuse of clean water and reuse/reduction of the use of plastics. The cross-sectional findings on the needs of education and awareness of sustainability in the community and companies are presented.Originality/valueThe findings provide evidence of the ability of photovoice method as a pedagogical tool to promote reflection and change in the university community and to introduce sustainability cross-sectional content in green campus curricula. This photovoice experiment is simple and feasible to implement and has a very low economic cost, as long as there are qualified educators.
The impact of social stratification on morbidity during the COVID-19 pandemic
Authors: Raghunath Nilanjan , Tan Tony 
Published: September 2020

PurposeSocioeconomic status (SES) has been known to be associated with many aspects of social life such as health. We argue that social stratification remains relevant in understanding differentials in health during a pandemic, as countries globally have encouraged or enforced social distancing and remote work measures.Design/methodology/approachBy examining data sources and news reports on the COVID-19 pandemic, we aim to highlight the relationship between SES and morbidity, through the ability to adopt social distancing measures and work remotely. Utilizing publicly available data from the Maryland Transport Institute and the US Census, we performed linear regressions on median income, social distancing index and percentage of individuals working from home.FindingsIndividuals with higher SES are more likely to have jobs that provide opportunities for remote work to be performed, which allows for social distancing. Comparatively, individuals with lower SES are more likely to be involved in jobs that cannot be performed remotely. The linear regression models suggest a positive moderate and significant correlation between median income and social distancing index (R2 = 0.4981, p-value < 0.001), and a positive weak and significant correlation between median income and remote work (R2 = 0.2460, p-value < 0.001).Research limitations/implicationsGovernments need to account for SES in policymaking to reduce inequalities in health.Originality/valueThe paper aims to improve the understanding of social stratification and morbidity through examining data on the COVID-19 pandemic.
Institutional complexity and CSR practices: evidence from a developing country
Authors: Diab Ahmed , Metwally Abdelmoneim Bahyeldin Mohamed 
Published: September 2020

PurposeThe study aims to investigate the appearance of corporate social and environmental responsibility (CSER) practices in a context where economic, communal and political institutions are highly central and competing with each other.Design/methodology/approachTheoretically, the study draws upon the institutional logics perspective and the theoretical concepts of logics centrality and compatibility to understand how higher-order institutions interact with mundane CSER practices observed at the case company's micro level. Empirical data were solicited in an Egyptian village community, where fishing, agriculture and especially salt production constitute the main economic activities underlying its livelihood. A combination of interviews, informal conversations, observations and documents solicits the required data.FindingsThereby, this study presents an inclusive view of CSER as practiced in developing countries, which is based not only on rational economic perspectives – as is the case in developed and stabilised contexts – but also on social, familial and political aspects that are central to the present complex institutional environment.Originality/valueThe reported findings in this study highlight the role of non-economic (societal) logics in understating CSER in African developing nations.
Aligning conflict brand stories with product types
Authors: Yin Chin-Ching , Chiu Hung-Chang , Hsieh Yi-Ching 
Published: September 2020

Purpose
Using classic literary theory pertaining to conflicts, this study aims to investigate the links of various types of conflicts and brand attitudes, in the context of brand stories for search and experience product types.

Design/methodology/approach
The authors used a survey of university students to test this study’s hypotheses.

Findings
The results reveal significant main effects of four conflict reversal stories on brand attitudes. The influences of man-against-self, man-against-man and man-against-society conflicts on brand attitude are greater for experience than for search products. In contrast, the influence of man-against-nature conflict is higher for search than for experience products.

Research limitations/implications
To generalize the results, this study should be replicated using more heterogeneous populations, setting the study in other cultures, testing actual brands and using a wider range of products.

Practical implications
The findings provide insights for marketers seeking to use appropriate conflicts in their brand stories to enhance customers’ brand attitudes.

Originality/value
Extant research does not address the relationship between different types of conflicts and customer attitudes, and the current study bridges this research gap.
Valuation for a Preemptive Right on the Private House for Lease in Korea Using the Binomial Option Pricing Model
Authors: Kim Sung Tak 
Published: September 2020

This paper investigates the value for a preemptive right on the private houses for lease in Korea which were supplied at Pankyo and Heoungduk Area in February 2006 and May 2007.
Using the price of the houses in the neighborhood area. Bundang and Youngtong as proxy variable, we estimated the value of a preemptive right based on the binomial option pricing model.
Major results are summarized as follows: i. While a purchase of right for the case of Pankyo were profitable, the case of Heoungduk wasn't which was consistent with the historical competition results; ii. while the profitability for the Pankyo was stable across the variation of the parameters, Heoungduk was not. Finally, some policy implications and limitations of this paper were suggested.
Scientific mapping on the convergence of innovation and sustainability (innovability): 1990–2018
Authors: De la Vega Hernández Iván Manuel , Barcellos de Paula Luciano 
Published: September 2020

Purpose
Innovation and sustainability are key in today’s competitive world. It is no longer possible to conceive an organization that has not incorporated these two elements for it to become successful. The longitudinal bibliometric study seeks to examine the topography of innovation and sustainability to determine whether there is already a terminological integration that could be defined as innovation capability in a sustainable way. The objective of this work is to identify the growth trajectories of the literature, the countries considered as knowledge nodes, the most influential authors, the classification of journals, clusters and collaborative networks measured by co-citation, the analysis by keywords, countries and knowledge areas.

Design/methodology/approach
The study is developed under the bibliometric approach, and the period 1990–2018 was considered. The steps taken were as follows: 16 keywords of each term were identified and selected in 3 methodological layers by a panel of experts; an algorithm was designed and applied to identify these selected keywords in the titles, abstracts and keywords using terms in the Web of Science (WoS) of Clarivate Analytics to contrast them; and a review in specialized journals was carried out in the first quartile (Q1) of the WoS until 2018.

Findings
The most relevant finding of the study lies in the sustained growth of the convergence of the terms innovation and sustainability. In addition, the longitudinal review of the two concepts also shows that innovation is directly linked to sustainability, suggesting that the convergence of concepts and practices becomes part of the strategy of companies seeking to be competitive and sustainable.

Practical implications
At the management level, this research seeks to present a definition of innovability based on the convergence of the concepts of innovation and sustainability. This new concept can be applied as a management strategy for companies seeking to be competitive and sustainable. Theoretical and practical evidences that reinforce this construct are presented. At the academic level, the manuscript shows a longitudinal review on innovation and sustainability, a relevant scientific mapping and, finally, a new theoretical and practical concept is proposed that seeks to create strategies for competitiveness in a sustainable way. At the level of society, the study contributes to the sustainable development goals.

Originality/value
The results show that a field of studies on innovation is emerging that has a high potential to position itself as a new space for research. The concept of “innovability” is proposed.