bluuebunny/crossref_metadata_2025_split huggingface dataset:
El espacio turístico en las arquitecturas radicales Actif au Maroc como paradigma
Authors: Manrique-Diaz Emili , Roig Eduardo , Seve Bruno 
Published: December 2024

La arquitectura de carácter vacacional que sirvió al turismo de masas a mediados del siglo pasado cuestionó sus estrategias proyectuales de forma pareja a las de la crisis de la modernidad. Aquella arquitectura moderna de carácter positivista que solventaba objetivamente problemáticas de alojamiento vacacional se fue transformando en una arquitectura rica en referencias a aspectos vinculados a la subjetividad de los usuarios, respondiendo a la vez a los discursos teóricos coetáneos de carácter utópico. La llamada ‘arquitectura radical’ es un ejemplo de esta mutación, y también lo fue de la arquitectura turística en particular, donde encontramos una heterogénea aproximación a los imaginarios turísticos que establece conexiones entre arquitectura y el carácter hedonista intrínseco a lo turístico a través de la experiencia creativa y corporal de los usuarios.El proyecto Actif au Maroc es un paradigma de las propuestas arquitectónicas de carácter turístico desde el ámbito de la arquitectura radical’. Redactado bajo la dirección de los arquitectos Herman Grubb y Till von Hasselbach en 1971, aglutinó transversalmente propuestas de carácter espacial, performático y tecnológico de diversos actores adscritos al círculo radical vienés en un contexto geográfico desértico situado al sur de Marruecos. Esta investigación analiza un proyecto poco documentado hasta la fecha, vinculándolo a experiencias coetáneas y mostrándolo como referente para la formalización del espacio turístico contemporáneo, un ámbito que hoy exhibe una creciente proyección hacia lo sensorial y lo lúdico, frente a la representación y la mirada propias de la Postmodernidad.
A QUÍMICA DOS REPELENTES: AÇÃO DA CITRONELA NA PREVENÇÃO DE ARBOVIROSES TRANSMITIDAS PELO AEDES AEGYPTI
Authors: Da Rocha Fernandes Erlane , Campos Rios Serra José Geraldo , Cunha Da Costa Ondina , Carvalho Da Costa Wherie , Machado Ângela Franciely , Scapin Elisandra 
Published: December 2024

O estudo aborda a ecologia química, investigando as interações entre organismos e o ambiente, com ênfase em repelentes à base de citronela e seu uso no combate a arboviroses. O projeto envolve a pesquisa sobre repelentes naturais como alternativa aos produtos químicos sintéticos no controle do Aedes aegypti, mosquito transmissor de doenças como dengue, zika, chikungunya e febre amarela. O objetivo do estudo foi aplicar um projeto de intervenção com alunos do EJA do Colégio Estadual Dom Alano Marie Du Noday em Palmas - Tocantins, visando ampliar o conhecimento dos educandos acerca de repelentes naturais, especialmente o óleo de citronela. Utilizando a metodologia ativa de Aprendizagem Baseada em Problemas, os alunos participaram de uma aula teórica seguida por um vídeo demostrando a extração do óleo essencial da citronela, posteriormente os discentes trabalharam em um jogo de palavras cruzadas elaborado pelos pesquisadores e ao final das atividades os alunos responderam um questionário de avaliação do projeto. Os resultados permitem inferir que a diversidade e a pertinência dos temas foram reconhecidas como enriquecedoras, embora alguns alunos tenham expressado dificuldades de aprendizagem, evidenciando a importância de uma abordagem pedagógica adaptativa. Quanto à aplicabilidade dos conteúdos no cotidiano, os alunos reconheceram sua utilidade prática e relevância, destacando a necessidade de elementos aplicáveis em diferentes contextos. A avaliação dos alunos foi efetivada durante o processo de ensino-aprendizagem e revelou uma compreensão sólida dos conceitos apresentados e uma percepção positiva da metodologia utilizada. Em síntese, os resultados indicam o sucesso da abordagem ao estimular a participação dos alunos e promover a construção do conhecimento sobre a citronela e suas aplicações.
TRANSPARÊNCIA DOS ATOS NAS CONTRATAÇÕES EMERGENCIAIS PELA GESTÃO PÚBLICA MUNICIPAL EM TEMPOS DE PANDEMIA DA COVID-19: UM ESTUDO DE CASO NO MUNICÍPIO DE PALMAS -TO
Authors: Antonio de Oliveira Andrade Alexandre , de Medeiros Ana Lúcia , Da Rocha Silva Mônica Aparecida 
Published: December 2024

A transparência nos atos da gestão pública é uma tônica atual e tem seu atendimento previsto em lei. O objetivo deste artigo foi medir a transparência da governança pública nas contratações emergenciais da Prefeitura de Palmas – TO, decorrente da pandemia da covid-19. O entendimento do nível de transparência em que a gestão municipal de Palmas se enquadra, foi a questão norteadora, para isso foi utilizada a técnica de pesquisa documental, a partir de dados consultados no site oficial da prefeitura municipal de Palmas. O método utilizado para ponderação dos dados adota o que estabelece a Transparência Internacional – Brasil. Os resultados demostraram que o site oficial da prefeitura em questão não dispõe de um ambiente específico para a concentração das informações relativas as contratações emergenciais de forma a facilitar a consulta por parte dos usuários que, além de atender às legislações, ainda facilite o controle social relativos aos atos da gestão pública. Ao final do trabalho foi atribuído resultado final de quarenta e cinco pontos, levando o site a ser classificado como regular.
Penerapan Metode Extreme Learning Machine (ELM) untuk Meramalkan Produksi Air Minum dan Air yang Dijual di Kota Yogyakarta Tahun 2023
Authors: Kireyna Cindy Pradhisa Kireyna , Yotenka Rahmadi 
Published: December 2024

Peramalan adalah teknik analisis dengan melibatkan data referensi/historis dari masa lalu untuk memprediksi kejadian dimasa mendatang. Air adalah suatu zat cair yang tidak berbau, tidak mempunyai rasa, warna, dan belum terjamin kebersihannya untuk dikonsumsi. Sedangkan air minum sendiri merupakan air yang telah melalui proses tahapan pengolahan yang sudah terjamin bersih dan aman untuk dikonsumsi/diminum. Semakin lama air minum sangat dibutuhkan bagi kehidupan masyarakat sehari-hari dan sudah menjadi kebutuhan pokok. Tujuan dari penelitian ini adalah untuk meramalkan banyaknya penjualan air minum dan air yang dijual di Kota Yogyakarta selama 12 periode kedepan. Data yang digunakan dalam penelitian ini adalah data time series dari Januari 2018 hingga Desember 2022. Penelitian ini menggunakan Software RStudio dan metode Extreme Learning Machine (ELM). Maka, didapatkan nilai Mean Absolute Percentage Error (MAPE) 6.854473 untuk peramalan data produksi air minum. Sedangkan pada peramalan data air yang dijual diperoleh nilai Mean Absolute Percentage Error (MAPE) 1.253288.
IbHAK5, a potassium transporter, enhances potassium ion absorption and improves root morphology in sweetpotato
Authors: Jin Rong , Zhao Peng , Yan Mengxiao , Liu Ming , Fan Weijuan , Zhang Qiangqiang , Zhu Xiaoya , Wang Jing , Yu Yongchao , Yang Jun , Wang Hongxia , Tang Zhonghou 
Published: December 2024

Abstract

Potassium is a vital element in sweetpotato that plays important roles during its growth and development. In this study, potassium transporter IbHAK5, which is homologous to Arabidopsis HAK5, was cloned and overexpressed in sweetpotato. IbHAK5 encodes a protein of 739 amino acids and localized in the plasma membrane. Two IbHAK5-overexpressing transgenic (HO) lines with the highest expression level of IbHAK5 were screened for K+-deficiency stress tolerant assay. Compared with wide type (WT) sweetpotato plants, transgenic plants grew well with higher chlorophyll content, and maintain great higher K+ contents via decreasing more K+ effluxes under low potassium ion (− K+) stress condition. Additionally, IbHAK5 can help plants improve root morphology and increase endogenous hormone IAA content under both normal condition and − K+ stress, which may result in the increased root K + absorption ability. The results indicated that IbHAK5 play an important role in sweetpotato response to – K+ stress, as well as support molecular-assisted breeding with the IbHAK5 gene.
Kaempferol enhances ER-mitochondria coupling and protects motor neurons from ER stress and mitochondrial dysfunction in C9ORF72- ALS
Authors: Pilotto Federica , Smeele Paulien , Scheidegger Olivier , Diab Rim , Schobesberger Martina , Sierra-Delgado Julieth Andrea , Saxena Smita 
Published: December 2024

Abstract

Repeat expansions in the C9ORF72 gene are a frequent cause of amyotrophic lateral sclerosis (ALS) and frontotemporal dementia. Considerable progress has been made in identifying C9ORF72-mediated disease and resolving its underlying etiopathogenesis. The contributions of intrinsic mitochondrial deficits as well as chronic endoplasmic reticulum stress to the development of the C9ORF72-linked pathology is well established, Nevertheless, to date, no cure or effective therapy is available, and thus attempts to find a potential drug target, have received increasing attention. Here, we investigated the mode of action and therapeutic effect of a naturally occurring dietary flavanol, kaempferol in preclinical rodent and human models of C9ORF72-ALS. Notably, kaempferol treatment of C9ORF72-ALS human patient-derived motor neurons/neurons, resolved mitochondrial deficits, promoted resiliency against severe ER stress and conferred neuroprotection. Treatment of symptomatic C9ORF72 mice with kaempferol, normalized mitochondrial calcium uptake, restored mitochondria function, and diminished ER stress. Importantly, in vivo, chronic kaempferol administration ameliorated pathological motor dysfunction and inhibited motor neuron degeneration, thus highlighting the translational potential of kaempferol. Lastly, in silico modelling identified a novel kaempferol target and mechanistically the neuroprotective mechanism of kaempferol is through the iP3R-VDAC1 pathway via the modulation of GRP75 expression. Thus, kaempferol holds great promise for the treatment of neurodegenerative diseases where both mitochondrial and ER dysfunction are causally linked to the pathophysiology.
Multi-omics analysis of Cuproptosis-Related Signature Guides the Precision Immunotherapy and Prognosis of Breast Cancer
Authors: zhang chenchen , yang jialai , yin Xin , zhang luxiao , Yu Tian-Hang , hao jiqing , zhang sidong 
Published: December 2024

Abstract

Breast cancer is a common malignancy in women, and its resistance to immunotherapy is a major challenge. Cuproptosis, a novel form of cell death associated with protein lipidation, tumor microenvironment, and immune response, may offer a potential solution. Aberrant expression of lncRNAs is important in BC initiation and progression, and the role of cuproptosis-related long non-coding RNAs (CRLs) in the immune regulation of BC is not fully understood. Breast cancer patient data from the TCGA database was used to identify cuproptosis-related genes (CRGs). Differential expression analysis of CRLs was performed using the 'limma' package in R. Prognostic CRLs were identified using co-expression and univariate Cox analysis. A prognostic model of six CRLs was established using Cox regression and the LASSO algorithm. Model performance was evaluated using K-M survival and ROC curve analysis. The predictive ability of the signature in immune microenvironment and immunotherapy was investigated. In vitro experiments validated AL138789.1 function. Our study identified a six-CRL prognostic signature that outperformed conventional clinicopathological characteristics in predicting survival outcomes in BC patients. The signature effectively stratified BC patients into high- and low-risk groups and showed potential in predicting the response to immunotherapy. Notably, significant differences were observed in immune cell abundance between the two groups. In vitro experiments demonstrated that AL138789.1 knockdown or overexpression significantly reduced the viability, proliferation, and invasion capacity of MDA-MB-231 or HCC1806 cells. Our 6-CRL signature has the potential as an independent biomarker for predicting prognosis and treatment response in BC patients, complementing existing clinicopathological characteristics.
Evaluation of dose dependent developmental response in Drosophila melanogaster to the diet fortified with Swarna Bhasma (Incinerated Gold).
Authors: Deshmukh Saylee , Sharbidre Archana , Choudhary Kuldeep , Reddy R Govind , Pawar Sharad 
Published: December 2024

Abstract

Background:
The gold-based Ayurvedic formulation Swarna Bhasma (SB) is renowned for its therapeutic applications, with a focus on pediatric treatment. Nevertheless, there is a scarcity of scientific evidence regarding its safety and efficacy across different dosages, particularly when used in conjunction with honey and ghee.
Objective:
This study aims to evaluate the dose-dependent developmental response of Drosophila melanogaster to diets fortified with Swarna Bhasma.
Methods:
SB was characterized employing X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR). Drosophila melanogaster larvae were fed diets with varying concentrations of SB (0.6–10 µg/ml). Changes in survivability, larval body weight, crawling behavior, pupation, and adult eclosion were monitored. Cellular damage and oxidative stress were assessed through the Trypan Blue Exclusion Assay and Nitroblue Tetrazolium (NBT) assay, respectively.
Results:
Moderate dose of SB (2.5 µg/ml) increased adult eclosion rates (121.7 ± 3.76%) and promoted normal crawling behavior. Higher doses (5 and 10 µg/ml) resulted in reduced pupation and eclosion rates, abnormal crawling, and significantly increased oxidative stress (37 ± 8.3%). No significant intestinal damage or phenotypic abnormalities were observed at any dose.
Conclusion:
SB demonstrates dose-dependent effects on Drosophila melanogaster development and behavior. Moderate doses are beneficial, while higher doses induce neurotoxic effects. These findings highlight the importance of determining safe and effective dosage for the therapeutic use of SB. Further research is needed to establish long-term safety and efficacy, particularly when used in combination with honey and ghee in Ayurvedic practices.
Generation of frameshift mutated TGFβR2-specific T cells in healthy subjects following administration with cancer vaccine candidate FMPV- 1/GM-CSF in a Phase 1 study
Authors: Inderberg Else Marit , Singh Nand , Miller Robert , Arbe-Barnes Sarah , Eriksen Henrik K. , lversen Berit , Juul Hedvig Vidarsdotter , Eriksen Jon Amund , Handeland Karianne Risberg 
Published: December 2024

Abstract

FMPV-1 is a component of FMPV-3, an investigational cancer-specific vaccine and being developed to activate anti-cancer T-cell responses targeting frameshift mutations of MSI-H cancers. FMPV-1 is designed to activate T-cell responses against transforming growth factor β receptor 2 (TGFβR2) frameshift mutation. Microsatellite instability High (MSI-H) gastrointestinal cancers frequently harbour TGFβR2 frameshift mutations. This first-in-human, Phase 1, single centre, open-label study included 16 healthy male subjects who received FMPV-1 (0.15 mg/injection) plus granulocyte macrophage colony stimulating factor (GM-CSF) (0.03 mg/injection) as two separate, co-located, injections on days 1, 8, 15, 29 and 43. All subjects were followed to Day 365.
A FMPV-1-specific delayed type hypersensitivity (DTH) skin reactivity test was performed with FMPV-1 (without GM-CSF) on days 1, 29 and 43 with assessment after 2 days. All subjects were DTH negative at baseline, 8/16 were positive on Day 31 and 15/16 were positive on Day 45. Furthermore, the FMPV-1/GM-CSF induced frameshift mutant TGFβR2 specific T cells after the short vaccination period, and specific T cells were still detectable after 6 and 12 months indicating induction of frameshift mutant TGFβR2 specific T memory cells. Adverse events were limited to mild injection-site reactions with no evidence of related systemic signs or symptoms. No other clinically important changes to vital signs, electrocardiograms, haematologic, coagulation or laboratory measures related to treatment were observed.
FMPV-1/GM-CSF was well tolerated and generated vaccine-specific T cell immune responses in healthy subjects. These findings support clinical studies in patients with, or at risk of, cancers carrying TGFβR2 frameshift mutations.
Clinical trial identification
ClinicalTrials.gov: NCT05238558.
EudraCT: 2020-004363-80.
PTOV1 Exerts Pro-oncogenic Role in Colorectal Cancer by Modulating SQSTM1-Mediated Autophagic Degradation of p53
Authors: Fan Yongli , Li Yuqin , Luo Xia , Xiang Shiqi , Hu Jia , Zhan Jingchun , Chang Weilong , Deng Rui , Ran Xianwen , Zhang Yize , Cai Yudie , Zhu Weiwei , Wang Huifen , Liu Zhibo , Wang Di 
Published: December 2024

Abstract

Background
The incidence and mortality of colorectal cancer (CRC) are increasing rapidly worldwide. Prostate Tumor Overexpressed 1 (PTOV1) is overexpressed and associated with malignant phenotypes in various types of tumors. However, the detailed roles of PTOV1 and its underlying mechanism in CRC remain unclear.
Methods
The clinical significance of PTOV1 was assessed in clinical databases (TCGA and GEO) and clinic CRC samples. The effects of PTOV1 on the tumor-associated phenotypes of CRC were detected by EdU-DNA synthesis assay, transwell assays, cell colony formation assay, CRC subcutaneous tumor model and CRC metastasis mouse models. Immunoprecipitation (IP) combined with protein mass spectrometry was used to identify potential molecules interacting with PTOV1. Co-Immunoprecipitation (Co-IP) was employed to validate the binding between PTOV1 and its interacting protein p53. Immunofluorescence assay, western blot and transmission electron microscopy (TEM) analysis were used to evaluated the effects of PTOV1 on autophagy.
Results
Here, we revealed that PTOV1 was highly expressed in human CRC tissues, especially at advanced stages, and associated with reduced survival time among CRC patients. The upregulated PTOV1 promoted cell proliferation, migration, invasion, tumor growth and metastasis of CRC cells in vitro and in vivo. At the molecular level, PTOV1 destabilized p53 by activating autophagy and recruiting p53 for the cargo receptor SQSTM1 directed autophagic degradation. There was a negative expression correlation between PTOV1 and p53 in CRC tissues. Moreover, p53 overexpression or SQSTM1 knockdown reversed the pro-tumor phenotypes of PTOV1 in CRC.
Conclusion
Our study reveals an oncogenic role of PTOV1 in CRC progression, which was achieved by promoting SQSTM1 directed autophagic degradation of p53. This work may provide promising therapeutic targets for CRC.
Irritability in Children with Rasopathies, Insights into Emotional Dysregulation and Social Skills Impairments
Authors: Serur Yaffa , Fuhrmann Naomi , Russo Odeya , Green Tamar 
Published: December 2024

Abstract

Rasopathies, including Noonan Syndrome (NS) and Neurofibromatosis type 1 (NF1), are developmental disorders caused by germline mutations in genes of the RAS/mitogen-activated protein kinase pathway (RAS-MAPK). This study investigates irritability, a highly prevalent transdiagnostic construct, in children with Rasopathies and the impact of Rasopathy status on the associations between irritability, emotional dysregulation-related disorders, and social skills impairments. The sample comprise 174 children aged 4–17 (age mean = 9.49; 98 females), including 113 children with Rasopathies (NS n = 85, NF1 n = 28) and 61 age-sex-matched typically developed (TD) children. We used parent questionnaires (CBCL, SRS) to assess irritability, symptoms of ADHD, defiance, anxiety/depression, and social skills impairments while controlling for cognitive measures (IQ). Children with Rasopathies exhibited higher irritability than TD children (mean difference = 1.09; p < 0.001). Children with NS showed a weaker association between irritability and ADHD symptoms compared to TD children (p = .032, ηp2 = .03) and a stronger association between irritability and social skills impairments compared to both TD (p = .033, ηp2 = .03), and NF1 groups (p = .009, ηp2 = .06). We present novel and clinically significant findings showing high irritability in children with Rasopathies. Our study provides syndrome-specific results, suggesting differences in the mechanisms involved in irritability, ADHD, and social processes in children with NS and NF1. In essence, children with Rasopathies showed a highly irritable profile associated with ADHD symptoms and social skills impairments, with a significantly stronger association between irritability and social processes in NS. Our results suggest that developing prevention and treatments targeting irritability can distinctly affect the trajectories of neurodevelopmental disorders in children with Rasopathies.
Hartnup disease combined with iminocaciduria: two novel mutations in the SLC6A19 gene (a case report)
Authors: Mei Ningning , Li Lei , Cui Yan , Wang Liang , Jiang Hong , Tian Xinying 
Published: December 2024

Abstract

Purpose
To report a case of Hartnup disease combined with iminocaciduria, and to explore its clinical manifestations and genetic mutations.
Methods
Clinical data of the patient were collected, whole-exome sequencing was performed using high-throughput sequencing technology to detect SLC6A19 gene mutations. Sanger sequencing was used for family verification, and software was employed to predict protein structure and function.
Results
The patient exhibited intermittent pellagra, ataxia, and psychiatric symptoms. Genetic sequencing revealed three heterozygous mutations in the SLC6A19 gene: a heterozygous missense mutation c.169C > T (p.Ary57Cys), a heterozygous missense mutation c.1802C > A (p.A601D), and a heterozygous splicing mutation c.1378 + 5G > A. The c.169C > T and c.1378 + 5G > A mutations were inherited from the father, whereas the mutation c.1802C > A was passed down from the mother. Bioinformatics-based protein function prediction indicated that both the c.169C > T (p.R57C) and c.1802C > A (p.A601D) mutations are harmful. Furthermore, the levels of 5-oxoproline and proline in the urine were elevated.
Conclusion
The typical manifestations of Hartnup disease include intermittent pellagra, ataxia, and psychiatric symptoms. The SLC6A19 mutations c.1802C > A and c.1378 + 5G > A are novel, and this case expands the genetic spectrum of the disease.
Performance Analysis of Bridges- Assessing Damage Impact and Performance Degradation
Authors: Chowdary T Anusha , Meghashree Mrs , Urs Neethu 
Published: December 2024

Abstract

This paper presents an investigation into the effects of bearing stiffness variations and vehicle speeds on the performance of bridges, with a focus on elastomeric bearings. Using finite element analysis (FEA), the study simulates the behaviour of a bridge subjected to varying load scenarios, including dead load + live load (DL+LL) and truck load conditions. Elastomeric bearings, modified at specific spans (99.6m, 182.6m, and 265.6m), are analysed for stiffness reductions (0.25%, 0.7%, and 0.9%) and their impact on bridge response. The results show that the central span (182.6m) experiences the highest displacement and stress, with peak displacement occurring around mid-span. As bearing stiffness decreases, axial forces and displacements increase, particularly under truck load conditions. This leads to higher stress concentrations in damaged bearings, which can compromise load distribution and bridge stability. The paper also explores the dynamic effects of varying vehicle speeds, revealing that higher speeds exacerbate displacement and stress. The findings emphasize the critical role of bearing stiffness in load transmission and the need for regular monitoring and predictive modelling to optimize bridge performance. This research provides valuable insights for improving maintenance practices and extending the service life of bridges through more accurate damage assessments and strategic interventions.