bluuebunny/crossref_metadata_2025_split huggingface dataset:
Overexpressing of POU2F2 accelerates fracture healing via regulating HMGA1/Wnt/β-catenin signaling pathway
Authors: Zhang Wei , Zhao Hanke , Chen Kun , Huang Ye 
Published: November 2019

ABSTRACTTo elucidate the role of POU2F2 (POU class 2 homeobox 2) in fracture healing, 30 rats with femoral fracture were randomly grouped into three groups: FF group, LV-POU2F2 group and LV-scramble group. Rats were injected with PBS, lentivirus expressing POU2F2 or scramble lentivirus once a week for 4 weeks. Results showed that overexpressing of POU2F2 promoted fracture healing and callus growth. Besides, overexpressing of POU2F2 promoted protein and mRNA expression of Col10a1, Runx2, Osterix, and Osteocalcin. High Mobility Group AT-hook 1 (HMGA1) is a non-histone protein participating in chromatin remodeling of cells. Western blotting manifested HMGA1/Wnt/β-catenin pathway was activated in POU2F2 group. Moreover, in-vitro study of hMSCs cells supported the above data. In conclusion, POU2F2 promotes fracture healing via activating the HMGA1/Wnt/β-catenin signaling pathway.
Quality and efficiency assessment of six extracellular vesicle isolation methods by nano‐flow cytometry
Authors: Tian Ye , Gong Manfei , Hu Yunyun , Liu Haisheng , Zhang Wenqiang , Zhang Miaomiao , Hu Xiuxiu , Aubert Dimitri , Zhu Shaobin , Wu Lina , Yan Xiaomei 
Published: November 2019

ABSTRACTExtracellular vesicles (EVs) have sparked tremendous interest owing to their prominent potential in diagnostics and therapeutics. Isolation of EVs from complex biological fluids with high purity is essential to the accurate analysis of EV cargo. Unfortunately, generally used isolation techniques do not offer good separation of EVs from non‐EV contaminants. Hence, it is important to have a standardized method to characterise the properties of EV preparations, including size distribution, particle concentration, purity and phenotype. Employing a laboratory‐built nano‐flow cytometer (nFCM) that enables multiparameter analysis of single EVs as small as 40 nm, here we report a new benchmark to the quality and efficiency assessment of EVs isolated from plasma, one of the most difficult body fluids to work with. The performance of five widely used commercial isolation kits was examined and compared with the traditional differential ultracentrifugation (UC). Two to four orders of magnitude higher particle concentrations were observed for EV preparations from platelet‐free plasma (PFP) by kits when compared with the EV preparation by UC, yet the purity was much lower. Meanwhile, the particle size distribution profiles of EV preparations by kits closely resembled those of PFP whereas the EV preparation by UC showed a broader size distribution at relatively large particle size. When these kits were used to isolate EVs from vesicle‐depleted PFP (VD‐PFP), comparable particle counts were obtained with their corresponding EV preparations from PFP, which confirmed again the isolation of a large quantity of non‐vesicular contaminants. As CD9, CD63 and CD81 also exist in the plasma matrix, single‐particle phenotyping of EVs offers distinct advantage in the validation of EVs compared with ensemble‐averaged approaches, such as Western blot analysis. nFCM allows us to compare different isolation techniques without prejudice.
Metabologically active hydrolytical microbial communities of soil ecosystems under influence of soil physical factors
Authors: Manucharova N A , Stepanov A L , Kozhevin P A , Zenova G M 
Published: November 2019

Abstract
Application of molecular biological techniques in environmental studies provides a more complete information concerning the taxonomic diversity and potential hydrolytic activity of soil microbial complexes that exist in a wide range of environmental factors. Among the key environmental parameters that determine the functional activity of the hydrolytic complex of soil layer, the most significant one is moisture. Moisture levels providing maximum activity of a hydrolytic microbial complex depend on the soil type. At high levels of moisture and temperature, the role of prokaryotic organisms, mainly actinomycetes, in the microbial complex significantly increases. It was discovered a new functional activity of actinomycetes in the hydrolytic prokaryotic complex: their controlling influence on the respiratory level of the complex in a wide range of parameters (moisture, organic matter, successional time).At the optimum for the life of most microorganisms levels of moisture (60% of field capacity) and temperature (27°C), representatives of Firmicutes and Actinobacteria phylums stand out among chitinolytic and pectinolytic dominants of the studied soils within the Bacteria domain. With increasing moisture and decreasing temperature the proportion of Proteobacteria increases. With decreasing moisture and increasing temperature, there is an increase in the amount of unicellular actinobacteria.
Comparison of four classical algorithms to determine the apparent thermal diffusivity of heavy clay soil in field and laboratory column experiments
Authors: Mikailsoy F D , Shein E V 
Published: November 2019

Abstract
Prediction of soil temperature at an acceptable level of accuracy is essential for managing soil temperature at large scales since its measurement is costly and not practical due to its high spatial and temporal variability. This study was carried out for:: 1) Calculate the coefficient of thermal conductivity using classical methods and basing on data from measured soil profile temperatures in column and field conditions and 2) compare the results of the calculated and measured soil temperature, calculated from these data for both conditions. Soil temperature was measured with thermal sensors on both soil profiles in the field and soil column. The measured temperature values were similar in soil column and profile across 0.05, 0.10, and 0.20 m depths, but had the difference for 0.30 cm. The introduction of the second harmonic allows determining with high accuracy the parameters (T0, Ti, εi) of the temperature distribution on the field and column soils surfaces. For example, respectively for the harmonics n = 1 calculated the following statistical characteristics: R2 = 86.45%, σ = 3.26, A = 13.07%, UII=0.12 and for n = 2: R2 = 98.64%, σ = 1.09, A = 4.20%, UII=0.04. It was found that, mean values for soil thermal diffusivity (k), thermal conductivity (λ), damping depth (d) and heat absorptivity (e), calculated by amplitude, arctangent and logarithm methods, for the column were greater than those for soil profile, in all the cases.
Increasing the efficiency of using renewable energy sources for energy supply of autonomous highland objects
Authors: Petrov Yu S , Khadikov M K , Zorina I Yu , Muzayev A K 
Published: November 2019

Abstract
Ways of increasing the efficiency of using renewable energy sources in the autonomous power supply of highland facilities are considered. Descriptions of autonomous multifunctional energy complexes that simultaneously use converters of various types: sunlight, wind and water flows into electrical energy, patented in NCIMM (STU) are given. The prospects of using renewable energy sources for autonomous power supply of highland facilities are substantiated.
Reserves of energy saving and reduction of oil recovery costs
Authors: Kolesnik S V , Krivova N R 
Published: November 2019

Abstract
The paper considers the use of controlled drive for oil recovery and pressure maintenance, which represents the oscillating system (sucker-rod pump drive) and adjustable drive of a pump unit (for pressure maintenance system) thus making it possible to reduce power consumption through oil recovery by 25…30 %. The development of electric drives, increase of their controllability, reduction of energy losses is considered alongside with the method of rotation speed control by redistribution of angular speed and power flows through a differential. In order to provide modern technological systems with cost efficient electric drives controlled by speed in a wide range with constant power on the output shaft, the new control principle (birotating) can be considered promising due to economy, simple design, low cost caused by lack of power electronics and high reliability.
Risks of long-term leasing transactions for construction industry development
Authors: Barykina Yuliya 
Published: November 2019

Abstract
The article examines potential long-term leasing risks for development of the construction industry, characterizes leasing as a form of investment. Leasing objects of the construction industry were identified. Methods for minimizing risks were considered. Leasing companies and large construction companies rarely lease out equipment to private, small-sized construction companies facing general and specific risks. The degree of risk depends on the leasing object (e.g., equipment that cannot be disassembled, rare equipment, expensive equipment which is difficult to sell at an affordable price). The problem to be solved is lack of owned funds for carrying out innovation activities. The article describes funding methods for innovation activities of manufacturing enterprises. It has been found that the most common funding method is leasing. Innovation activities of enterprises are a number of measures aimed to search for, commercialize and implement scientific knowledge, new technology and inventions. Currently, innovation activities influence the Russian economy by enhancing efficiency of resource management, creating new industries, infrastructure and jobs. Under the global competition, the effects of innovation activities on national development and competitiveness are becoming more intensive.
State programs on provision of population with affordable and comfortable housing: an issue of efficient implementation
Authors: Grushina Olga , Shelomentseva Natalya 
Published: November 2019

Abstract
the paper deals with the issue of state-supported provision of the population with affordable and comfortable housing. Over the last decade the organizational and economic mechanism of state participation in solving social problems involves mainly development, approval and implementation of various state programs. In the present paper, evolution of the state programs in the field of affordable housing adopted since 1991 is discussed in detail. The goals and objectives of the programs, target indicators and their changes over time, and the possibility of their attaining are critically analyzed. The authors have tried to find ways to assess the efficiency of the state programs implementation from the point of view of those for whom they are devoted to, i.e. citizens who need to improve their living conditions. Some indicators for assessing the dynamics of changes in the number of households that are in need of housing and meeting the requirement of the programs are calculated. The analysis shows that the state programs are of low efficiency for those whose housing problems should be solved. The applied methods for evaluating the efficiency of state programs exist “per se”, and for the management they are considered as quite satisfactory. However, in the Irkutsk region, the effectiveness of housing state programs in accordance with the applied methodology for 2015-2017 is only 70-80%. Recommendations for improving the efficiency of state programs should be made in two directions: (i) improvement of the budget planning of the region and (ii) improvement of the target indicators of the programs.
Smart Specialization of Russian Regions: Prospects and Limitations
Authors: Kalyuzhnova Nadezhda , Violin Sergei , Matveeva Maria 
Published: November 2019

Abstract
The article deals with the possibilities of integrating the concept of smart specialization into regional development policies in the Russian Federation. Strategies of smart specialization are being actively and successfully developed in the regions of the European Union. Smart specialization implies setting priorities for economic development based on local strengths and resources. The strategy assumes the ‘entrepreneurial discovery process’, during which promising market niches are identified along with technologies and innovations that a region can develop in order to gain competitive advantages in those niches. The article analyzes the required institutional framework for smart specialization implementation. Propositions are made on how Russian institutions in the fields of innovations and regional development can adapt to further the smart specialization agenda. The article concludes with the analysis of the draft strategy for social and economic development of the Irkutsk oblast from the point of view of its conformity with the criteria of smart specialization. Specific guidelines are outlined for the improvement of the strategy in this respect.
Low-water demand cements – a reliable way of CO2 emission reduction
Authors: Khozin Vadim , Khohryakov Oleg , Baishev Daniil , Makarenko Sergey 
Published: November 2019

Abstract
This paper presents results of performance comparison and common conclusions about low-water demand cements (LWDC) which were developed in USSR in 1980-ies. Using technology of this composite binders allows to dramatically decrease CO2 emission without loss of performance. In current study different parameters of production have been studied such as types of admixtures, mineral fillers, workability parameters, e.g., milling ability with superplasticizers solutions as grinding agents and compressive strength. It is set that during first period of grinding any cement systems which contain water will perform lowered grinding ability, but after some tine this parameter begins to grow and up to values, equal to systems without water. This fact makes possible to use, e.g. water solutions of PCE, and allows to decrease cost of LWDC in order to make it cost-effective.
Mortgage as a way to purchase housing in modern conditions
Authors: Kudryavtseva Vera 
Published: November 2019

Abstract
The main goal of any government is to create a system that would solve social problems. One of these problems is affordable and comfortable housing. Property prices are traditionally high enough. In this regard, most of the buyers are forced to take out a mortgage as one of the alternative ways to solve the housing problem. Mortgage allows a buyer to purchase an apartment or a house and pay its cost in installments over the next years.
Industrial Construction: Investment Support in Entrepreneurship
Authors: Matveeva M V , Kalyuzhnova N Y 
Published: November 2019

Abstract
The article deals with the main directions of solving the problem of insufficient investment attractiveness of industrial construction in the Irkutsk region. A lot of research is devoted to the improvement of investment attractiveness and competitiveness of construction projects. Investments are considered not only as a source for economic growth, but also as a factor of competitiveness of regional economy and construction. The article highlights the main problems and prospects of development to raise the investment attractiveness of regional construction projects. The author has reviewed the impact of investment attractiveness on the formation of con-competitive advantages of a territory. Increasing the investment attractiveness of industrial construction can be achieved through the implementation of a set of measures providing for: the development of the regulatory and legislative framework governing investment relations in construction; improvement of the organizational and economic mechanism, including tools for managing investment projects in industrial construction; integration of programs of the state support and stimulation of creation of industrial parks, construction of objects of infrastructure, engineering preparation in borders of the land plot allocated for building.
Remote mortgage lending as a resource for housing development
Authors: Peshkov A V 
Published: November 2019

Abstract
The article describes the causes enough active use of the mechanism of the mortgage in order to resolve housing issues. It emphasizes that the problem of formation and development of the property market and the use of such forms of financing investments in real estate as mortgage lending is one of the most urgent today. In this regard, using the experience of developed countries, conclusions about the prospects of development of legal institutions in our country. Consideration of a mortgage as an intangible resource has a high degree of relevance in terms of further regulating mortgage as a banking product, as well as selecting the direction of development of state policy in the field of residential construction.
Legal protection of BIM products
Authors: Stepanenko Olga , Tatarnikov Vladimir , Cherdakova Larisa 
Published: November 2019

Abstract
Science and technology are developing faster than intellectual property legislation. As a result, previously unknown products of intellectual activity are regulated by general rules. These objects are BIM products. The use of BIM technology in the construction industry involves creation of a three-dimensional model filled with architectural, design, technological, economic and other data about a building considered as a single object. The BIM product may include several intellectual property objects regulated by different laws (e.g., databases and design inventions, know-how, etc.). The legislation of the Russian Federation does not define the concept “BIM products”. There are no integrated objects taking into account its features. It is not possible to apply Article 1240 of the Civil Code of the Russian Federation to the BIM products. The list of intellectual property objects is limited. It is necessary to include BIM products into Article 1240 of the Civil Code of the Russian Federation.
Structural, optical and impedance spectroscopy study of thin film of polyaniline (PANI/ZnO) nanocomposite
Authors: Shah Mutabar , Khan Noman , Imran Zahid , Khan Masood , Khattak Abraiz , Khan Adam , Ullah Naimat 
Published: November 2019

Abstract
Modern organic electronic and power electronic devices gained attention due their superior performance and efficiency with reduced cost. While nanocomposites of these organic semiconductors have high potential to further enhance these properties. In this work, thin films of zinc oxide-polyaniline (ZnO-PANI) nanocomposite with different compositions of ZnO (50, 60 and 70 wt%) are deposited by spin coater onto the surface of p-Si. The phase identification of the samples is identified through x-ray diffraction analysis. Pure ZnO showed wurtzite crystalline while PANI and nanocomposites of ZnO-PANI showed amorphous structure. The optical absorption band gap was evaluated from Ultraviolet–visible spectroscopy absorption studies and band gaps were calculated from Tauc’s plots. The Ultraviolet–visible spectroscopy showed that by increasing ZnO content in the nanocomposite, the band gap increases from 3.25 eV to 3.3 eV and 3.4 eV for 50–50, 60–40 and 70–30 wt% respectively. The AC electrical properties of the nanocomposites were studied by using impedance spectroscopy. The parameters extracted from the impedance plots and conductivity values showed that 50–50 wt% is more conductive while that of 70%–30% wt is more resistive.
Not Being Able to Speak Is Torture: Performing Listening to Painful Narratives
Authors: Sotelo Castro Luis Carlos 
Published: November 2019

Abstract
This Note discusses a new creative approach to intervene in the interaction between survivors who contribute their memories of a painful past and the public they want to reach. It proposes that by turning both the narrator and the public into performers of listening, they are placed in a relational context that offers an alternative space in which victims’ and the public’s memories, emotions and thoughts in a transitional context can be worked through. The method thereby corrects the notion of the public as a disembodied, virtual totality underlying state-led transitional justice institutions.
Human Rights at the Time of Transition: How Security Forces Can be Held Accountable in a Divided Community?
Authors: Qafisheh Mutaz M 
Published: November 2019

Abstract
The Palestinian Authority has established various mechanisms to monitor its security forces and hold them accountable over human rights violations. This article explores and assesses the accountability measures that deal with the human rights abuses arising from the excessive use of force in light of international standards, particularly human rights treaties that Palestine has acceded to in recent years. The use of force may occur in different contexts, including during demonstrations, while enforcing the law against criminal acts, searches, lawlessness, in detention, interrogation, investigation and in prisons. The article traces the accountability processes that arise in such circumstances. Alongside reviewing domestic legislation and cases as well as citing relevant literature, the article employs empirical qualitative field research approach by conducting a series of interviews with senior security officials, particularly top commanders and those in charge of accountability within the government and security agencies along with NGOs, experts, academics and field-based international institutions.
Modeling the Growth of Black Soldier Fly Hermetia illucens (Diptera: Stratiomyidae): An Approach to Evaluate Diet Quality
Authors: Sripontan Yuwatida , Chiu Chun-I , Tanansathaporn Soisunee , Leasen Kittiya , Manlong Kuntida 
Published: November 2019

Abstract
The black soldier fly (BSF, Hermetia illucens (Linnaeus), Diptera: Stratiomyidae) is an edible insect widely reared by using various types of organic wastes as its diet. Developing tools to evaluate the growth performance of BSF is crucial for improving its production. In this study, we monitored the daily growth of BSF larvae reared with 10 diets with diverse nutritional quality and compared 7 growth models for quantifying growth performance. We found that BSF generally exhibited an S-shaped growth curve and that the Richards model was the best-fitting growth model. We estimated asymptotic growth limit (W∞) and constructed a time-series curve of absolute growth rate (AGR) by using the Richards model. We used Gaussian functions to estimate AGR parameters (maximum AGR, m; timing of maximum AGR, T; the deviation of AGR, d) to evaluate whether these parameters adequately quantify the growth performance of BSF. Correlation analysis showed that the AGR parameters were mostly independent of W∞ but were correlated with the duration of the larval stage, indicating that W∞ and the AGR parameters respond to different dietary factors. The results of pairwise comparisons showed that the diets rich in carbohydrates and protein had high W∞, m, and d, and early T. The diets with low levels of carbohydrates had low W∞, and the diets with low levels of protein had low m and d and delayed T. We conclude that the parameters estimated by the Richards model could be effective indices for evaluating the growth performance of BSF and their diet quality.