bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Matching Status Between Donor and Recipient Hepatitis B Seroepidemiology Makes a Difference in Liver Transplantation for Hepatocellular Carcinoma
Authors: Lu Di , Yang Fan , Zhuo Jianyong , Yang Modan , Lin Zuyuan , Jin Pingbo , Cai Xuechun , Cen Beini , Wang Jianguo , Wei Xuyong , Zheng Shusen , Xu Xiao 
Published: May 2020

INTRODUCTION:
Antibody to hepatitis B core antigen (HBcAb) is known to be related with the prognosis for patients with hepatocellular carcinoma (HCC). This study aims to evaluate the prognostic capacity of HbcAb and other donor/recipient hepatitis B seroepidemiological indexes in transplantation for HCC.

METHODS:
Based on the national liver transplant registry, we analyzed the prognostic capacity of HBcAb in liver transplantation for patients with HCC of different etiological backgrounds. The hepatitis B virus (HBV)-related HCC cohort was further studied regarding donor/recipient hepatitis B seroepidemiology, and then divided into a training cohort (n = 1,222) and a validation cohort (n = 611) to develop a pretransplant recurrence-risk predicting nomogram.

RESULTS:
Positive HbcAb in recipients was related to an increased risk of post-transplant tumor recurrence in HBV-related (n = 1,833, P = 0.007), HCV-related (n = 79, P = 0.037), and non-B non-C HCC (n = 313, P = 0.017). In HBV-related HCC (n = 1,833), donor hepatitis B surface antigen (HbsAg) was also associated with post-transplant tumor recurrence (P = 0.020). Multivariate analysis showed that the matching status of recipient HbcAb and donor HbsAg (MSHB) was an independent prognostic factor (P = 0.017). HbcAb-positive recipients matched with HbsAg-positive donors displayed the worst post-transplant outcomes (P < 0.001). In the training cohort (n = 1,222), a risk-predicting nomogram was established based on α-fetoprotein, Milan criteria, and MSHB. The model showed excellent prognostic capacity and safely expanded Milan criteria in both training and validation cohorts (P < 0.001).

DISCUSSION:
Positive HbcAb in recipients increases the risk of post-transplant tumor recurrence in HCC with different etiological backgrounds. The nomogram based on MSHB is effective in predicting tumor recurrence after transplantation for HBV-related HCC.
Revue illustrée des principales indications de CBCT en orthodontie.
Authors: Olszewski Raphael , Theys Stéphanie , Perez Eytan , Wisniewska Katarzyna , Wisniewski Marcin 
Published: May 2020

Cette revue illustrée porte sur les principales indications actuellement recommandées dans la littérature d’utilisation du cone beam computed tomography (CBCT) en orthodontie.
Il s’agit des anomalies dentaires, des canines incluses, des dents surnuméraires, des troubles de l’éruption et des résorptions radiculaires externes liées aux traitements orthodontiques.
L’examen CBCT doit être justifié individuellement, au cas par cas, et de pouvoir apporter un bénéfice au patient en terme de diagnostic et/ou de traitement orthodontique.
L’orthodontiste prescripteur doit être capable d’interpréter et est responsable de l’interprétation de tout ce qui est visible sur l’ensemble du champs de vue du CBCT.
Identifikasi Pepper vein yellows virus yang Berasosiasi dengan Penyakit Yellow Vein Banding pada Tanaman Mentimun di Bali
Authors: Nyana I Dewa Nyoman , Lestariningsih M. Tinny , Adnyani Ni Nengah Putri , Suastika Gede 
Published: May 2020

Yellowing vein banding disease has been reported infecting cucurbit plants in Bali since 2014. Similar vein banding symptom on chilli pepper was observed previously, and early diagnosis indicated infection of Polerovirus. The objective of this research was to confirm the presence of Polerovirus infection on cucumber plant showing yellow vein banding symptom in Bali. Reverse transcription polymerase chain reaction – based detection method was conducted using specific primer pairs PeVYV-CP-F-BamH1/ PeVYV-CP-R-Pst1followed by sequencing and nucleotide sequence analysis. Specific DNA fragments of ± 650 bp was successfully amplified from field samples. Nucleotide sequence analysis showed that the sequence has the highest similarity > 95% with Pepper vein yellow virus (PeVYV) infecting chili pepper from Indonesia (Bali, and Rembang), Japan, and Greece.
Pengendalian Colletotrichum spp. Terbawa Benih Cabai dengan Paparan Gelombang Mikro
Authors: Najah Lilih Naelun , Suhartanto Mohamad Rahmad , Widodo Widodo 
Published: May 2020

Seed treatment using microwave has been reported as an effective and efficient method to control seed borne pathogens of chili pepper. The purpose of this research was to evaluate the effectiveness of microwave irradiation treatment to suppress seedborne Colletotrichum spp. while maintaining physiological quality of chili’s seed. First experiment was aimed to select the best condition for seed germination, and was done in completely randomized design with three levels of water content (4.31%, 6.33%, and 8.25%). The second experiment was aimed to determine the best condition for disease suppression, and was done in completely randomized design with different levels of microwave irradiation duration (0, 10, 20, 30, 40, and 50 seconds) using the best seed water content level from the first experiment. Application of systemic fungicide with benomyl as active ingredient (0.5 g L-1) was done as check treatment. Four major species of Colletotrichum was found from chili’s seed, i.e. C. acutatum, C. capsici, C. gloeosporioides, and Colletotrichum sp. with C. acutatum as the predominant species. The best water content level for microwave treatment was 4.31%. The most effective treatment was microwave irradiation duration for 40 seconds with efficacy rate of 64.5% for controlling seedborne C. acutatum on chili pepper.
Cendawan Endofit yang Potensial Meningkatkan Ketahanan Cabai Merah terhadap Penyakit Layu Bakteri
Authors: Irawati Ana Feronika Cindra , Sastro Yudi , Sulastri Sulastri , Suhartono Maggy Tenawidjaja , Mutaqin Kikin Hamzah , Widodo Widodo 
Published: May 2020

Ralstonia solanacearum has been known to cause bacterial wilt disease on chili pepper. Despite many reports on the potential use of endophytic fungi to induce plant resistance, its utilization to suppress bacterial wilt disease of chili has not been widely reported. The aims of this research was to screen potential endophytic fungi that may increase chili resistance against bacterial wilt disease. Selection of endophytic fungi was done using in vivo and antibiosis test. Strains of fungi were considered the most potent in suppressing the development of bacterial wilt in chilli were identified. Ten out of 62 isolates of endophytic fungi gave the highest suppression on chilli’s bacterial wilt disease. Most strains of endophytic fungi were able to suppress the development of bacterial wilt disease, but not always positively correlated to the vegetative and generative growth of chilli. Based on the level of disease intensity and the growth of plants were obtained three strains of endophytic fungi that considered potentially suppress the incidence of bacterial wilt disease. The three isolates was identified as Fusarium solani f.sp. phaseoli (AC-2.13 and AC-4.4) and Trichoderma asperellum (AC-3.18) using morphology and molecular characters. Although all three selected isolates were able to suppress bacterial wilt disease in this study, but application of F. solani f.sp. phaseoli should be considered in practical use since it is generally known as the causal agent of root rot disease of beans
Endocrinology in the time of COVID-19: Management of pituitary tumours
Authors: Fleseriu Maria , Dekkers Olaf M , Karavitaki Niki 
Published: May 2020

AbstractPatients with pituitary tumours, ensuing hormonal abnormalities and mass effects are usually followed in multidisciplinary pituitary clinics and can represent a management challenge even during the times of non-pandemic. The COVID-19 pandemic has put on hold routine medical care for hundreds of millions of patients around the globe, while many pituitary patients' evaluations cannot be delayed for too long. Furthermore, the majority of patients with pituitary tumours have co-morbidities potentially impacting the course and management of COVID-19 (e.g. hypopituitarism, diabetes mellitus, hypertension, obesity and cardiovascular disease). Here, we summarize some of the diagnostic and management dilemmas encountered, and provide guidance on safe and as effective as possible delivery of care in the COVID-19 era. We also attempt to address how pituitary services should be remodelled in the event of similar crises, while maintaining or even improving patient outcomes. Regular review of these recommendations and further adjustments are needed, depending on the evolution of the COVID-19 pandemic status. We consider that the utilization of successful models of pituitary multidisciplinary care implemented during the COVID-19 pandemic should continue after the crisis is over by using the valuable and exceptional experience gained during these challenging times.
Population Differentiation at the PVT1 Gene Locus: Implications for Prostate Cancer
Authors: Pal Gargi , Di Lia , Orunmuyi Akintunde , Olapade-Olaopa E Oluwabunmi , Qiu Weigang , Ogunwobi Olorunseun O 
Published: May 2020

Abstract
Genetic variation in susceptibility to complex diseases, such as cancer, is well-established. Enrichment of disease associated alleles in specific populations could have implications for disease incidence and prevalence. Prostate cancer (PCa) is a disease with well-established higher incidence, prevalence, and worse outcomes among men of African ancestry in comparison to other populations. PCa is a multi-factorial, complex disease, but the exact mechanisms for its development and progression are unclear. The gene desert located on chromosome 8q24 is associated with aggressiveness of PCa. Interestingly, the non-protein coding gene locus Plasmacytoma Variant Translocation (PVT1) is present at chromosome 8q24 and is overexpressed in PCa. PVT1 gives rise to multiple transcripts with potentially different molecular and cellular functions. In an analysis of the PVT1 locus using data from the 1000 Genomes Project, we found the chromosomal region spanning PVT1 exons 4A and 4B to be highly differentiated between African and non-African populations. We further investigated levels of gene expression of PVT1 exons 4A and 4B and observed significant overexpression of these exons in PCa tissues relative to benign prostatic hyperplasia and to normal prostate tissues obtained from men of African ancestry. These results indicate that PVT1 exons 4A and 4B may have clinical implications in PCa a conclusion supported by the observation that transient and stable overexpression of PVT1 exons 4A and 4B significantly induce greater prostate epithelial cell migration and proliferation. We anticipate that further exploration of the role of PVT1 exons 4A and 4B may lead to the development of diagnostic, therapeutic, and other clinical applications in PCa.
Diseño de un prototipo de protector facial con una mascarilla electrónica para la protección del COVID19
Authors: Larico Yaneth Carol , López Oscar Mauricio Flores 
Published: May 2020

El presente artículo describe el diseño de un prototipo de protector facial con una mascarilla electrónica para la protección del COVID 19. Este diseño cuenta con un sistema de filtrado de aire y un sistema de ventilación, recargable por medio de un puerto USB, que permite a los portadores del protector facial con mascarilla electrónica, una mejor oxigenación y refrigeración mientras realizan actividades laborales (salud, policial y militar) como también actividades cotidianas, brindando de este modo una mejor sensación térmica. Los resultados obtenidos luego de efectuar el sistema de ventilación y filtrado se adaptan de manera eficiente y se encuentra en el nivel medio de ventilación con un consumo de ronda 200mA, lo que se adecúa a la mayoría de personas evitando así una sobre ventilación por un elevado flujo de aire.
Características clínicas, radiológicas y de laboratorio en niños con diagnóstico de COVID-19: Metaanálisis de proporción única
Authors: Vasco-Morales Santiago , Vasco-Toapanta Cristhian Santiago , Toapanta-Pinta Paola 
Published: May 2020

Objetivo: Resumir la mejor evidencia disponible acerca de la frecuencia de presentación de: signos, síntomas, principales exámenes de laboratorio y, alteraciones radiológicas en pacientes menores de 19 años con diagnóstico de COVID-19.
Método: Mediante búsquedas en PubMed, Scopus y Google académico, se recopilaron los datos de los estudios que presentaban las características clínicas, de laboratorio y radiología, para realizar metaanálisis de proporción única.
Resultados: Se encontró 11 estudios, que incluían un total de 1180 pacientes. El meta-análisis presentó una mayor afectación del sexo masculino 0.56(IC 95%; 0.51:0.61), pacientes asintomáticos 0.15(IC 95%; 0.09:0.22), infecciones leves y muy leves 0.44(IC 95%; 0.25:0.63), infecciones moderadas 0.44(IC 95%; 0.38:0.45), incidencia de fiebre 0.56(IC 95%; 0.48:0.65), tos 0.46(IC 95%; 0.39:0.53), faringitis 0.18(IC 95%; 0.03:0.39), en los exámenes de laboratorio presentó mayor incidencia la leucopenia 0.33(IC 95%; 0.25:0.42), la Proteína C reactiva 0.19(IC 95%; 0.15:0.24), los pacientes con alteración radiológica 0.68(IC 95%; 0.56:0.79), pacientes graves n=5 y fallecidos n=1.
Conclusiones: La presencia de anormalidades radiológicas es mas frecuente que los signos clínicos de tos y fiebre que son los síntomas más comunes. En el hemograma: la leucopenia es mas frecuente. Existe una tendencia de mayor afectación en el sexo masculino. Este metaanálisis servirá de base para futuras comparaciones acerca de las similitudes o diferencias que pueden presentarse ahora que la pandemia está en América. También presenta limitaciones por lo que los resultados deben ser contrastados, con estudios prospectivos controlados, con mayor número de pacientes y un diseño mas estricto.
RECOMENDACIONES DE LA ASOCIACION COLOMBIANA DE MEDICINA DE SUEÑO PARA EL EJERCICIO DE LA MEDICINA DEL SUEÑO EN COLOMBIA DURANTE LA CONTINGENCIA PANDEMIA COVID-19.
Authors: Zabala-Parra Sandra Irene , Venegas Marco , Amado-Galeano Yhon Steve , Escobar-Córdoba Franklin , Restrepo-Gualteros Sonia Maria , Tavera-Saldaña Lina Marcela , Bazurto-Zapata Maria Angélica , Echeverry-Chabur Jorge 
Published: May 2020

La Asociación Colombiana de Medicina del Sueño (ACMES) con el fin de ayudar a los somnólogos a evaluar la unidad de sueño y los procedimientos que se llevan a cabo en la misma en respuesta al brote del COVID-19 emite la presente guía. Esta se fundamenta en las estrategias de mitigación recomendadas por las autoridades sanitarias del país y de la revisión bibliográfica de la evidencia médica disponible y recomendaciones actuales AASM. Colombia se encuentra en la fase de transmisión sostenida del COVID-19 y las medidas de lavado de manos frecuente, de uso de mascarilla por la población general en sitios públicos, de distanciamiento físico y confinamiento en los hogares, respaldada por normas sanitarias locales han ayudado a frenar la propagación del virus. Sin embargo, la crisis económica asociada a este brote hace urgente que el país retorne poco a poco con la implementación de las medidas de bioseguridad necesaria a una relativa normalidad. En este contexto actual de la pandemia en Colombia la ACMES ha diseñado esta guía para que los somnólogos y las unidades de sueño locales tengan una orientación mínima en su práctica durante este brote y una vez superadas las medidas de salud pública para bajar la velocidad del contagio del virus, reabrir poco a poco la economía y contener la velocidad de la propagación para volver a la normalidad en lo posible.
A comparative study on the general health of the mothers of children with cochlear implant, hearing aid, and normal hearing
Authors: Gohari Nasrin , Sajadi Ebtesam , Azvantash Zoleikha , Khavarghazalani Bahare 
Published: May 2020

Background and Aim: The general health of the mothers of children using hearing aids is highly important, and can affect their children’s progress in hearing rehabilitation programs. This study aimed to compare the general health of the mothers of children using cochlear implants (CIs), mothers of children using hearing aids (HAs), and mothers with normal hearing (NH) children.
Methods: In this descriptive-comparative study conducted on the mothers of children using CIs (n = 19), HAs (n = 19), and NH (n = 15). Their general health was measured by the 28-item general health questionnaire (GHQ-28) in four areas of physical symptoms, anxiety symptom, social dysfunction symptom, and depression symptom.
Results: The general health of the mothers of children using CIs was significantly higher than that of the mothers with children using HAs (p = 0.02), while it was lower than that of the mothers with NH children, but it was not statis­tically significant (p = 0.5).
Conclusion: Mothers of children using CI chil­dren have higher general health than the mothers with children using HA, and its level was close to that of the mothers with NH children. Cochlear implantation and consequently improved comm­unication is effective in improving the general health of the hearing-impaired children’s moth­ers.
Keywords: Hearing aid; cochlear implant; general health questionnaire; mothers
The Relationship of Food Insecurity and Quality of Life with Helicobacter Pylori Infection
Authors: Talezadeh Shirazi Soudeh , Eftekhari Mohammad Hassan , Ahmadi Afsane , Mazloom Zohreh , Fararouei Mohammad , Ranjbar Zahedani Maryam 
Published: May 2020

Background: Helicobacter pylori (H pylori) is the most prevalent human infection in half of the world population. Few studies investigated the reasons of infection to H pylori in societies. The current study was designed i to examine the relationship of food insecurity and quality of life with H pylori infection. Methods: In this case- control study, 200 candidates were selected among the people who referred to Shiraz Imam Reza Clinic. They were asked to complete the general questionnaire, food security, and quality of life questionnaires. The H pylori excremental diagnosis test was also taken from them.
Results: The prevalence of H pylori infection was 28%. No relationship was observed between H pylori and age, gender, household size, and body mass index. However, the prevalence of H pylori infection was significantly higher in married individuals and housewives and volunteers with lower educations and those who took digestive medications. In addition, food insecurity (P = 0.04) and low quality of life (P < 0.001) were significantly high in individuals with H pylori infection. However, the possibility of suffering from H pylori infection in individuals who consume digestive medicines was 3.97 times higher than other individuals (95% CI: 2.11-7.40). Furthermore, with reduction of quality of life, the possibility of suffering from H pylori infection increased 1.77 times (95% CI: 1.203-2.591).
Conclusion: The data indicated a statistically significant increased risk of H pylori in marital status, occupation, educational level, quality of life, and food insecurity in the affected individuals. The association of food insecurity and quality of life with H pylori infection should be examined in future studies in different populations
CRISPR Gene Editing on Human Embryos
Authors: Sefid Fatemeh , Khadempar Saedeh , Shamriz Roshanak , Amjadi Nooshin 
Published: May 2020

Background: With the recent development of CRISPR/Cas9 genome editing technology, the possibility to genetically influence the human germline (gametes and embryos) has become a separate technical possibility. As a powerful skill for genome engineering, the CRISPR/Cas9 system has been effectively applied to adjust the genomes of several species. The purpose of this review was to appraise the technology and build concepts for the launch of precise hereditary modifications in early human embryos.

Methods: We conducted a systematic review of the related literatures searched from PubMed, Google scholar, Web of Science up to June 30, 2017 and then we extracted the essential data. In this review, we present the brief history and basic mechanisms of the CRISPR/Cas9 system and significant challenges and advances in the field as a comprehensive practical guide to absorbed users of genome editing technologies. We introduce factors that influence CRISPR/Cas9 efficacy which must be addressed before effective in vivo human embryo therapy can be realized .in this review, we highlight the advancements that have been made using CRISPR/Cas9 in relation to Human Embryo.

Results and Conclusion: The possibility of CRISPR/Cas9 use in the context of human reproduction, to change embryos, germline cells, and pluripotent stem cells are studied created on the writers' expert belief. We discuss recent developments leading to the operation of Human Embryonic gene therapies in clinical trials and consider the predictions for future advances in this rapidly developing field.
Assembling reads improves taxonomic classification of species
Authors: Tran Quang , Phan Vinhthuy 
Published: May 2020

Abstract
Background: Most current metagenomic classifiers and profilers employ short reads to classify, bin and profile microbial genomes that are present in metagenomic samples. Many of these methods adopt techniques that aim to identify unique genomic regions of genomes so as to differentiate them. Because of this, short-read lengths might be suboptimal. Longer read lengths might improve the performance of classification and profiling. However, longer reads produced by current technology tend to have a higher rate of sequencing errors, compared to short reads. It is not clear if the trade-off between longer length versus higher sequencing errors will increase or decrease classification and profiling performance.Results: We compared performance of popular metagenomic classifiers on short reads and longer reads, which are assembled from the same short reads. When using a number of popular assemblers to assemble long reads from the short reads, we discovered that most classifiers made fewer predictions with longer reads and that they achieved higher classification performance on synthetic metagenomic data. Specifically, across most classifiers, we observed a significant increase in precision, while recall remained the same, resulting in higher overall classification performance. On real metagenomic data, we observed a similar trend that classifiers made fewer predictions. This suggested that they might have the same performance characteristics of having higher precision while maintaining the same recall with longer reads.Conclusions: This finding has two main implications. First, it suggests that classifying species in metagenomic environments can be achieved with higher overall performance simply by assembling short reads. This suggested that they might have the same performance characteristics of having higher precision while maintaining the same recall as shorter reads. Second, this finding suggests that it might be a good idea to consider utilizing long-read technologies in species classification for metagenomic applications. Current long-read technologies tend to have higher sequencing errors and are more expensive compared to short-read technologies. The trade-offs between the pros and cons should be investigated.