bluuebunny/crossref_metadata_2025_split huggingface dataset:
Polymorphisms in endoplasmic reticulum aminopeptidase genes are associated with cervical cancer risk in a Chinese Han population
Authors: Li Chuanyin , Li Yaheng , Yan Zhiling , Dai Shuying , Liu Shuyuan , Wang Xia , Wang Jun , Zhang Xinwen , Shi Li , Yao Yufeng 
Published: April 2020

Abstract
Background: Antigen-processing machinery molecules play crucial roles in infectious diseases and cancers. Studies have shown that polymorphisms in endoplasmic reticulum aminopeptidase (ERAP) genes can influence the enzymatic activity of ERAP proteins and are associated with the risk of diseases. In the current study, we evaluated the influence of ERAP gene (ERAP1 and ERAP2) polymorphisms on susceptibility to cervical intraepithelial neoplasia (CIN) and cervical cancer. Methods: Six single nucleotide polymorphisms (SNPs) in ERAP1 and 5 SNPs in ERAP2 were selected and genotyped in 556 CIN patients, 1072 cervical cancer patients, and 1262 healthy control individuals. Candidate SNPs were genotyped using SNaPshot assay. And the association of these SNPs with CIN and cervical cancer was analysed. Results: The results showed that allelic and genotypic frequencies of rs26653 in ERAP1 were significantly different between cervical cancer and control groups (P=0.001 and 0.004). The allelic frequencies of rs27044 in ERAP1 and rs2287988 in ERAP2 were significantly different between control and cervical cancer groups (P=0.003 and 0.004). Inheritance model analysis showed that genotypes of rs27044, rs26618, rs26653 and rs2287988 SNPs may be associated with the risk of cervical cancer (P=0.003, 0.004, 0.001 and 0.002). Additionally, haplotype analysis results showed that the ERAP1 haplotype, rs27044C-rs30187T-rs26618T-rs26653G-rs3734016C, was associated with a lower risk of cervical cancer (P=0.001). The ERAP2 haplotypes rs2549782G- rs2548538A-rs2248374A-rs2287988G-rs1056893T (P=0.009 and 0.006) and rs2549782T-rs2548538T-rs2248374G-rs2287988A-rs1056893T (P=0.003 and 0.009) might be associated with cervical cancer and the development from CIN to cervical cancer. Conclusion: Our results indicated that rs27044, rs26618 and rs26653 in ERAP1 and rs2287988 in ERAP2 influenced susceptibility to cervical cancer.
Magnitude of Turnover Intention and Associated Factors Among Nurses Working in Emergency Departments of Governmental Hospitals in Addis Ababa, Ethiopia: A cross sectional institutional based study.
Authors: Aniley Andualem Wubetie , Taye Biniyam , Girma Biruk 
Published: April 2020

Abstract
Background: The turnover intention is a probability of an employee to leave his or her current job within a certain period due to various factors. It is the strongest predictor of actual turnover which is expected to increase as the intention increases. Emergency Department(ED) nurses are especially vulnerable to turnover because of their increased potential for developing burnout and compassion fatigue in which the work environment has a great role. Objective : To assess the magnitude and associated factors that affect turnover intention among nurses working in emergency departments of selected governmental hospitals in Addis Ababa, Ethiopia. Methods: Institutional based cross-sectional study was conducted on 102 nurses in three selected governmental hospitals, Addis Ababa from February 19 to March 31, 2018, using a structured pre-tested self-administered questionnaire. Logistic regression model was used and adjusted odds ratio with a 95% confidence interval was calculated to identify associated factors. Result: A total of 102 respondents were involved with a response rate of 91.1%. Among them, 79 (77.5%) respondents had the intention to leave the current working unit of the emergency department or hospital. Significant predictive factors of nurses' intention to leave their jobs are educational status (adjusted odds ratio (OR) =4.700, 95% confidence interval (CI)=1.033- 50.772; p <0.048), monthly income of Less than 3145 ETB (adjusted OR=6.05, 95% CI=1.056-34.641; p <0.043) and professional autonomy (adjusted OR=0.191, 95% CI=0.040- 0.908; p <0.037). Conclusion: more than 75% of the respondents has intention to leave their current working place of emergency unit. Educational status; monthly income and autonomy were significantly associated with emergency nurses‟ turnover intention in three governmental hospitals. Emergency leaders and hospital managers should have made efforts to enhance nurses' decision making for patient care activities and shared decision overwork or unit related activities.
Knockdown of hsa_circ_0074298 suppressed the progression of pancreatic cancer by targeting the miR-519/SMOC2 axis
Authors: Chen Hong , Xu Dafang , Wang Lishan , Zhou Jiahua 
Published: April 2020

Abstract
BACKGROUND: Pancreatic cancer (PC) is among the most malignant tumors in digestive system because of its fast progression, metastasis, as well as its resistance to chemotherapy. Former studies found that circRNAs that were differentially expressed were correlated with progression and gemcitabine (GEM)-resistance in PC, and hsa_circ_0074298 aberrant expression played a role in PC progression, but the regulatory mechanism was unclear. METHODS: The RT-qPCR was used to detect hsa_circ_0074298 expression in PC cell lines. The CCK8, colony formation, and Transwell assays were used to evaluate the effect of hsa_circ_0074298 on PC cell migration and proliferation. Bioinformatics and luciferase reporter experiments were used to characterize the regulatory mechanism. Nude mouse xenografts were generated to examine the effect of hsa_circ_0074298 on tumor growth. RESULTS: The present study showed that hsa_circ_0074298 expression increased significantly in both PC cell lines and PC tissues. Downregulation of hsa_circ_0074298 caused a significant decrease in PC cell proliferation and in nude mouse xenograft growth. Hsa_circ_0074298 silencing also increased chemotherapy sensitivity to GEM. Bioinformatics analysis indicated that hsa_circ_0074298 was a miR-519 sponge and that the SMOC2 gene was a miR-519 target. MiR-519 downregulation or SMOC2 overexpression restored cell proliferation, migration, and chemoresistance after hsa_circ_0074298 silencing. The dual luciferase reporter assay showed that hsa_circ_0074298 interacted with miR-519, and miR-519 binding to the SMOC2 3′-UTR, then suppressed posttranscriptional SMOC2 expression. CONCLUSIONS: Taken together, hsa_circ_0074298 functioned as a tumor promoter through a novel miR-519/SMOC2 axis, highlighting its possibility as a potential therapy for PC.
Fluorescence labeling of a NaV1.7-targeted peptide for near-infrared nerve visualization
Authors: Gonzales Junior , Pirovano Giacomo , Chow Chun Yuen , Franca Paula Demetrio de Souza , Carter Lukas M. , Klint Julie Kaae , Guru Navjot , Lewis Jason S. , King Glenn F. , Reiner Thomas 
Published: April 2020

Abstract
Background Accidental peripheral nerve injury during surgical intervention results in a broad spectrum of potentially debilitating side effects. Tissue distortion and poor visibility can significantly increase the risk of nerve injury with long-lasting consequences for the patient. We developed and characterized Hs1a-FL, a fluorescent near-infrared molecule for nerve visualization in the operating theater with the aim of helping physicians to visualize nerves during surgery. Hs1a was derived from the venom of the Chinese bird spider, Haplopelma schmidti, and conjugated to Cy7.5 dye. Hs1a-FL was injected intravenously in mice and harvested nerves were imaged microscopically and with epifluorescence.Results Hs1a-FL showed specific and stable binding to the sodium channel NaV1.7, present on the surface of human and mouse nerves. Hs1a-FL allowed epifluorescence visualization of sciatic mouse nerves with favorable nerve-to-muscle contrast.Conclusions Fluorescent NaV1.7-targeted tracers have the potential to be adopted clinically for the intraoperative visualization of peripheral nerves during surgery, providing guidance for the surgeon and potentially improving the standard of care.
Supervisors’ intention to observe clinical task performance: an exploratory study using the Theory of Planned Behaviour during postgraduate medical training
Authors: de Jonge Laury PJWM , Mesters Ilse , Govaerts Marjan JB , Timmerman Angelique A , Muris Jean WM , Kramer Anneke WM , Vleuten Cees PM van der 
Published: April 2020

Abstract
Background Direct observation of clinical task performance plays a pivotal role in competency-based medical education. Although formal guidelines require supervisors to engage in direct observations, research demonstrates that trainees are infrequently observed. Supervisors may not only experience practical and socio-cultural barriers to direct observations in healthcare settings, they may also question usefulness or have low perceived self-efficacy in performing direct observations. A better understanding of how these multiple factors interact to influence supervisors’ intention to perform direct observations may help us to more effectively implement the aforementioned guidelines and increase the frequency of direct observations. Methods We conducted an exploratory quantitative study, using the Theory of Planned Behaviour (TPB) as our theoretical framework. In applying the TPB, we transfer a psychological theory to medical education to get insight in the influence of cognitive and emotional processes on intentions to use direct observations in workplace based learning and assessment. We developed an instrument to investigate supervisors intention to perform direct observations. The relationships between the TPB measures of our questionnaire were explored by computing bivariate correlations using Pearson’s R tests. Hierarchical regression analysis was performed in order to assess the impact of the respective TPB measures as predictors on the intention to perform direct observations. Results In our study 82 GP supervisors completed our TPB questionnaire. We found that supervisors had a positive attitude towards direct observations. Our TPB model explained 45% of the variance in supervisors’ intentions to perform them. Normative beliefs and past behaviour were significant determinants of this intention.Conclusion Our study suggests that supervisors use their past experiences to form intentions to perform direct observations in a careful, thoughtful manner and, in doing so, also take the preferences of the learner and other stakeholders potentially engaged in direct observations into consideration. These findings have potential implications for research into work-based assessments and the development of training interventions to foster a shared mental model on the use of direct observations.
Prevalence of positive TST among Healthcare workers in high-burden TB setting in Peru
Authors: Sedamano Juana , Schwalb Alvaro , Cachay Rodrigo , Zamudio Carlos , Ugarte-Gil Cesar , Soto-Cabezas Gabriela , Munayco César V. , Seas Carlos 
Published: April 2020

Abstract
Background: Tuberculosis (TB) transmission has long been recognized as an important occupational hazard for healthcare workers (HCWs). HCWs possess a 5.8% annual risk of exposure and three times greater risk of developing active TB than the general population.Methods: We conducted an observational cross-sectional study between September 2014 and March 2015 among HCWs in a high-burden TB setting in Lima to estimate the prevalence of positive Tuberculin Skin Test (TST) and to investigate factors associated with a positive TST.Results: 240 participants were included in the analysis; TST was administered to 190 (79.2%) while the rest were exempt due to a previous positive TST result, history of TB or refused the test. A positive TST result was found among 56.2% of participants who were applied the TST (95% CI: 49.22% - 63.55%). When considering those who had a previous positive TST result and those with a history of TB, the prevalence was 64.3% (95% CI: 57.8% - 70.3%). No significant differences were observed between clinical/paramedical and administrative staff in the health center. The use of N95 mask during work hours was reported by 142 (69.9%) participants. Prevalence ratios (PR) show that workers with more than 120 months as a HCW were 1.44 times more likely to be TST positive. The multivariate analysis found that HCWs with over 10 years of service were 1.52 times more likely to be TST positive.Conclusion: This study supports previous reports that TB infection is an occupational hazard for HCWs. Prevention of TB transmission through control measures, as well as timely diagnosis of LTBI in this selected risk group, is critical for the individual and for public health.
Increased serum calpain activity is associated with HMGB1 levels in systemic sclerosis
Authors: Zheng Ji-Na , Li Yang , Yan Yue-Mei , Yu Yong , Shao Wen-Qi , Wang Qiang 
Published: April 2020

Abstract
Background: Systemic sclerosis (SSc) or scleroderma is an intractable autoimmune disorder that affects multiple organs. The objectives were to investigate clinical correlations of serum calpain activity and high mobility group box 1 (HMGB1) levels with immunological and clinical traits. Methods: A total of 31 patients with SSc, 20 age- and gender-matched healthy control subjects (HC) and 10 patients with other connective tissue diseases (CTD) were recruited in the study. We measured serum calpain activity and HMGB1 levels and analyzed the datasets (GSE40839, GSE48149, GSE76808, GSE81292, GSE33463, and GSE58095) from gene expression omnibus (GEO) database to explore the potential mechanism by which calpain exerts its function through bioinformatics methods. Results: Serum calpain activity was significantly increased in patients with SSc compared with those in HC and in patients with CTD and was correlated with serum HMGB1 levels, modified Rodnan skin score, erythrocyte sedimentation rate, mean platelet volume, and plateletcrit. Notably, serum calpain activity and HMGB1 levels in SSc patients with interstitial lung disease (ILD) were significantly higher than those in SSc patients without ILD. Serum calpain activity and HMGB1 levels could be the independent risk factors for SSc-ILD and novel biomarkers in patients with SSc. Conclusion: This is the first study that reports increased serum calpain activity and the correlation between calpain and HMGB1 in patients with SSc or SSc-ILD. The serum calpain activity and HMGB1 levels may serve as measures of ILD in patients with SSc. Also, calpain and HMGB1 could be potential therapeutic targets for patients with SSc or SSc-ILD in the future.
Concerns around public health recommendations on face mask use among individuals who are not medically diagnosed with COVID-19 supported by a systematic review search for evidence.
Authors: Marasinghe Keshini Madara 
Published: April 2020

Abstract
BackgroundContradicting and inconsistent public health recommendations regarding face mask use have been provided to individuals who are not yet medically diagnosed with COVID-19, which is significantly a large population. Face masks are being used by individuals who are not medically diagnosed with COVID-19 as a means to limit the spread of COVID-19 in several countries around the world. While some countries recommend the use of face masks, other countries strictly do not recommend their use to limit the transmission of COVID-19 among individuals who are not medically diagnosed with COVID-19. This paper critically analyses public health recommendations provided to this population regarding face mask use by public health and health professionals of different countries supported by a systematic review that searched for evidence on face mask use among this specific population in limiting the spread of COVID-19.MethodsTo carry out the systematic review portion of this paper, databases Cochrane Library, EMBASE, Google Scholar, PubMed, and Scopus were searched for relevant studies. Two groups of keywords were combined: those relating to face masks and COVID-19.ResultsThe systematic review search did not find any studies that investigated the effectiveness of face mask use in limiting the spread of COVID-19 among those who are not medically diagnosed with COVID-19 to support current public health recommendations.ConclusionsThe finding of the systematic review search, which is a lack of scientific evidence, questions the basis of inconsistent public health recommendations that have been provided to the public at a very early yet a crucial stage of an outbreak. A closer attention need to be given to the procedures and practices behind providing public health guidelines and recommendations during an outbreak by public health and health professionals around the world. This paper calls for 1) evidence-based public health recommendations; 2) considerations when providing public health recommendations in the absence of evidence; 3) evidence and knowledge transparency on current public health recommendations; 4) global alignment on public health recommendations; and 5) further research to strengthen public health recommendations.
Effect of the Amount of Correction on Posterior Tibial Slope and Patellar Height in Open-wedge High Tibial Osteotomy
Authors: wu keliang , zeng jianchun , jie ke , feng wenjun , lin xiaosheng , chen jinlun , zeng yirong 
Published: April 2020

Abstract
Objectives The effect of amount of correction on postoperative changes in the posterior tibial slope (PTS), patellar height (PH) and clinical outcomes following biplanar open-wedge high tibial osteotomy (OWHTO) were evaluated. Method This study included 64 knees (30 left, 34 right) of 64 patients (mean age 48.5±6.8 years, 20 males, 44 females) with varus malalignment and symptomatic isolated medial joint osteoarthritis who underwent OWHTO. According to the amount of correction angles, all patients were divided into three groups: large correction angle (LCA) group (>14°), medium correction angle (MCA) group（10°～14°）and small correction angle (SCA) group (<10°). All patients were clinically assessed according to the Lysholm score, hospital for special surgery knee score (HSS), and knee society score (KSS) prior to and after surgery. For radiographic analysis, we measured the PTS，PH [Insall-Salvati index (ISI), Blackbume-Peel index (BPI)]. The pre-post difference of PTS, ISI and BPI was calculated by subtracting the post-OWHTO value to the pre-OWHTO value in three groups respectively. The preoperative, postoperative, and difference of PTS, ISI and BPI values were analyzed according to the correction angle. The mean follow-up period was 27.7 months(SD, 3.9; range 12-49 months). Results Radiologically, PTS increased and PH decreased after surgery on the whole ( p ＜0.05). The relationship between amount of correction and slope increasing is significant ( p ＜0.001). Furthermore, the pairwise difference between the LCA group and SCA group, MCA group are significant respectively ( p ＜0.05). In terms of PH, the LCA group yielded ISI and BPI that were significantly different from baseline for SCA group and MCA group. In addition, the pairwise difference between the SCA group and LCA group in ISI and BPI are significant ( p =0.031). Clinically, significant improvements were observed in postoperative clinical scores of the Lysholm score, HSS, and KSS ( p <0.05). Fifty-nine patients (92.2%) reported satisfaction with surgery. However, no correlation was found between changes in PTS and PH with postoperative knee score. No severe adverse complications were observed. Conclusions The amount of correction angle is a significant factor affecting the PTS and PH in OWHTO. With increased correction angle, the likelihood of increasing the PTS and decreasing the PH increases. Special attention should be paid to keep PTS and PH unchanged in cases where large corrections are required. Otherwise, closing wedge osteotomy or other intraoperative effective measures are supposed to be adopted.
Percutaneous vertebroplasty for Kummell’s disease: Short-term analysis of therapeutic effects and outcome predictors
Authors: Li Yibing , Wang Xiaodong , Gao Wenjie , Hao Dingjun 
Published: April 2020

Abstract
Objective: To retrospectively investigate the short-term therapeutic effect of percutaneous vertebroplasty (PVP) for Kummell’s disease using a variable angle needle and to analyze the possible outcome predictors. Material and methods: Twenty-seven patients were included in this study. Medical records were evaluated. The following factors were analyzed on imaging data: location of lesion, the percentage of bone marrow edema, patterns of cement spreading, and post-procedural kyphosis correction. Visual analogue score (VAS) was used to analyze the clinical effect, and the improvement rate was calculated. Statistical analyses were conducted to evaluate the relationship between those factors and therapeutic outcomes using Fisher’s exact test, Chi-squared test, and Student’s un-paired t-test. Results: The effectiveness rate was 70.4% at 1 week, 74.1% after 1 month, and 63.0% after 3 months. Six months and one year after procedures, the effective rate remained steady at 70.4%. Post-procedural kyphosis correction ≥8° was a risk factor for inferior pain relief. An area of edema over 40% was a prognostic factor for superior pain relief at the first follow-up point. Cement morphology was a positive predictor for better pain relief at 1 month follow-up. Conclusions: Compared to the therapeutic effects of PVP on fresh osteoporotic vertebral compression fractures (OVCF), percutaneous vertebroplasty for Kummell’s disease was effective in only 63% to 74.1% of the patients at different follow-up points. Overemphasized kyphotic correction (over 8 degrees) was associated with poor outcomes. Areas of edema over 40% and cement morphology of mixed type were positive prognostic factors in the early stage.
Ketorolac tromethamine pretreatment suppresses sufentanil-induced cough during general anesthesia induction: a prospective randomized controlled trial
Authors: Tian Zhen , Hu Bei , Miao Min , Zhang Lulu , Wang Lin , Chen Bin 
Published: April 2020

Abstract
Background: To observe the effect about pretreatment of ketorolac tromethamine on sufentanil-induced cough in general anesthesia patients. Methods: 102 patients were screened, and a total of 90 patients were scheduled for elective surgery under general anesthesia. 90 patients were randomly divided into two groups: the control group（C group）and the observation group（KT group）. 5 minutes before anesthesia induction, observation group were given ketorolac tromethamine 0.5mg/kg intravenously within 3 s, while the control group were given the same amount of normal saline intravenously. All patients were given a sufentanil bolus of 0.5 ug/kg（within 3 s）intravenously,1 minute later, propofol 2.5mg/kg, vecuronium 0.15mg/kg were injected intravenously, and endotracheal intubation was guided by laryngoscope. The number of coughs that occurred within 1 min after sufentanil injection were recorded. The mean arterial pressure (MAP)，heart rate (HR) and pulse oxygen saturation(SpO2) were recorded at T0 (immediately before pretreatment), T1 (5 minutes after pretreatment), T2（before intubation），T3 (1 min after intubation) and T4 (5 min after intubation).The incidence of adverse reactions were analyzed. Results: Within 1 min after sufentanil injection, the incidence and severity of cough in KT group was significantly lower than that in C group ( P < 0.05). At the time points of T0, T1, T2, T3 and T4, there was no significant difference in MAP, HR and SpO2 between the two groups ( P > 0.05). And there was no significant difference in the dosage of sufentanil, propofol, remifentanil and vecuronium, incidence of nausea and vomiting, delay of recovery, dizziness, drowsiness and respiratory depression between the two groups( P > 0.05). However, there were significant differences in the number of restlessness during waking period ( P < 0.05). Conclusion: Pretreatment of intravenous ketorolac tromethamine can significantly reduce the incidence of sufentanil-induced cough during induction period of general anesthesia patients, and can also significantly reduce the restlessness during recovery period of patients. Trial registration: Chinese Clinical Trial Registry (registration number# ChiCTR2000030287; date of registration: 27/02/2020).