bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nutritional and safety evaluation of locust (Caelifera) powder as a novel food material
Authors: Ochiai Masaru , Inada Mako , Horiguchi Seiya 
Published: January 2020

AbstractInsects are considered edible food resources with sufficient nutrients, but their nutrient composition and safety evaluation have not been fully investigated yet. In this study, we investigated the nutrient composition and the acute and sub‐chronic toxicity of locust powder in male rats. In the acute oral toxicological experiment, rats were administered locust powder at a dose of 10 or 20 g/kg/dose, followed by monitoring general signs of toxicity for 14 days. In the sub‐chronic toxicological experiments, rats were fed with a diet containing 1% and 3% locust powder for 28 and 90 days. General signs of toxicity, body weight, plasma and blood components, weight and fat accumulation in tissues, and fecal fat excretion were investigated. The locust powder was rich in proteins, essential amino acids, minerals, and polyunsaturated fatty acids. In the acute toxicological experiment, no general signs of acute toxicity were observed at a dose of 20 g/kg. In the sub‐chronic toxicological experiments, parameters related to red blood cell were lowered by the 3% locust powder for 28 days, but not for 90 days. Liver lipid accumulation and fecal fat excretion were increased by the 3% locust powder for 90 days, but the liver lipids contents were considered to be within a nontoxic level. Cecum contents and cecum short‐chain fatty acids were lowered by the locust powder, which can be caused by its fiber and fiber‐like components. In conclusion, acute and sub‐chronic intake of locust powder had little effect on general, biochemical, and hematological signs of toxicity in rats.Practical ApplicationEdible insects are increasingly viewed as new sustainable protein sources for human foods and livestock feeds worldwide because of their high nutritional balance, high food conversion rate, and environmental merits. Here, we have clarified that a locust powder contains high levels of protein, polyunsaturated functional fatty acids, and minerals (iron, zinc, and magnesium), and intake of locust powder (3% in diet) had little effects on general, biochemical, and hematological signs of toxicity in male rats. Locust as an edible insect, in powder form, can contribute to human dietary needs.
Factors associated with accessing aged care services in Australia after approval for services: Findings from the historical cohort of the Registry of Senior Australians
Authors: Inacio Maria C. , Amare Azmeraw T. , Whitehead Craig , Bray Sarah C. E. , Corlis Megan , Visvanathan Renuka , Wesselingh Steve 
Published: January 2020

AbstractObjectiveTo evaluate the access of approved aged care services and factors associated with accessing these services.MethodsA retrospective cohort study was conducted (1/7/2003‐30/6/2013). The incidence of accessing permanent residential, home and respite care services within one year or transition care within 28 days of approval was evaluated. The association of participants’ socio‐demographic characteristics, limitations, health conditions and assessment characteristics with service use was evaluated.ResultsIn 799 750 older Australians, the incidence of accessing approved permanent residential care within one year was 70.9% (95% confidence interval [CI] 70.8%‐71.0%), home care 49.5% (95% CI 49.3%‐49.7%) and respite 41.8% (95% CI 41.7%‐41.9%). The incidence of accessing transition care at 28 days was 78.5% (95% CI 78.2%‐78.7%). Aged care seekers’, assessments’ and assessors’ characteristics are associated with service access.ConclusionMonitoring the use of aged care service approvals is necessary for the identification of service access barriers to support evidence‐based policy changes.
Strategies to persist in the community: Soil seed bank and above‐ground vegetation in Patagonian pine plantations
Authors: Rago María Melisa , Urretavizcaya María Florencia , Orellana Ivonne Alejandra , Defossé Guillermo Emilio 
Published: January 2020

AbstractQuestionsIn terms of biodiversity conservation, forest plantations are often considered problematic because they usually have negative effects on natural ecosystems. Soil seed banks could play an important role in restoring vegetation that was originally present, by recruiting species that have disappeared above‐ground. We addressed this topic in plantations located in the Patagonian steppe by asking the following questions: Are richness, abundance and composition of steppe above‐ground vegetation and soil seed bank affected by plantations? Is species composition of the above‐ground vegetation and soil seed bank similar in plantations and in the steppe?LocationNW of Chubut Province, Patagonia, Argentina.MethodsWe selected three plantations located in the steppe, and delimited three sectors: steppe, plantation edge and plantation interior. We compared richness and abundance by species origin and growth form among sectors for the above‐ground vegetation and soil seed bank compartments, using generalized linear mixed models. We compared species composition for each compartment and between them using permutational multivariate analysis of variance and non‐metric multidimensional scaling.ResultsIn above‐ground vegetation, we found a decrease in richness and abundance, and changes in composition from the steppe to the plantation interior. Richness and abundance of the soil seed bank did not show differences among steppe and the plantation sectors, although composition was different among them. Above‐ground vegetation and soil seed bank species composition were always different; native perennial herbs and shrubs were predominant in the above‐ground vegetation while exotic annual herbs dominated the soil seed bank.ConclusionsOur findings suggest that plant recruitment from the soil seed bank would not be sufficient for restoring depleted species pools in the above‐ground vegetation of plantations. Therefore, actions promoting native species reestablishment and control of exotics should be considered to reach the aim of restoring biodiversity in former plantations.
Mortality associated with Fournier’s gangrene remains unchanged over 25 years
Authors: Radcliffe Robert S. , Khan Masood A. 
Published: January 2020

ObjectivesTo report a case series of Fournier’s gangrene (FG) from our institution, and to investigate its mortality over the past 25 years.Patients and MethodsCase notes of men presenting to our institution from 2013 to 2016 with FG were reviewed. As well as age, diabetic history, length of stay, length of stay in critical care, and mortality; we calculated (where possible) the Uludag Fournier’s Gangrene Severity Index (UFGSI).Published studies and cases series reporting the mortality rates for FG were reviewed from 1993 to 2018. The size of the study, country of origin, average age and gender ratio were collected, alongside mortality.ResultsTwo of the 11 patients treated for FG at out institution died within 90 days of admission, a mortality rate of 18%. Predicted mortality was significantly higher.A total of 173 publications were identified from the Medical Literature Analysis and Retrieval System Online (MEDLINE) database published between 1993 and 2018, reporting data from 1975 to 2018. Analysis of heterogeneity, by both time and precision, supported exclusion of four retrospective coded database‐driven studies from the analysis.From the remaining studies, mortality ranged from 0% to 42%. Of the 6152 reported cases, there were 1220 deaths, giving an overall mortality rate of 19.8%. There was no evidence of a significant change in the mortality rate for FG over time (P = 0.996).ConclusionsIn our case series, the mortality rate for FG was 18%, despite a higher predicated mortality (based on UFGSI scores). The treatment of FG remains appropriate resuscitation, aggressive surgical debridement, and critical care management. Perceived high risk of mortality should not deter aggressive management.Mortality due to FG does not appear to have changed over the past 25 years, and is estimated at 19.8%. In studies identifying cases of FG, careful attention should be paid to case definition, particularly when cases are being abstracted retrospectively from large coded databases.
Acid‐sensing ion channel 3 blockade inhibits durovascular and nitric oxide‐mediated trigeminal pain
Authors: Holton Christopher M. , Strother Lauren C. , Dripps Isaac , Pradhan Amynah A. , Goadsby Peter J. , Holland Philip R. 
Published: January 2020

Background and PurposeThere is a major unmet need to develop new therapies for migraine. We have previously demonstrated the therapeutic potential of the acid‐sensing ion channel (ASIC) blockade in migraine, via an ASIC1 mechanism. ASIC3 is expressed in the trigeminal ganglion and its response is potentiated by NO that can trigger migraine attacks in patients. Thus we sought to explore the potential therapeutic effect of ASIC3 blockade in migraine.Experimental ApproachTo investigate this, we utilised validated electrophysiological and behavioural rodent preclinical models. In rats, ASIC3 blockade using APETx2 (50 or 100 μg·kg−1, i.v.) was measured by using durovascular and NO‐evoked trigeminal nociceptive responses along with cortical spreading depression models. In mice, we sought to determine if periorbital mechanical sensitivity, induced by acute nitroglycerin (10 mg·kg−1, i.p.), was attenuated by APETx2 (230 μg·kg−1, i.p.), as well as latent sensitisation induced by bright light stress in a chronic nitroglycerin model.Key ResultsHere, we show that the ASIC3 blocker APETx2 inhibits durovascular‐evoked and NO‐induced sensitisation of trigeminal nociceptive responses in rats. In agreement, acute and chronic periorbital mechanosensitivity induced in mice by nitroglycerin and subsequent bright light stress‐evoked latent sensitivity as a model of chronic migraine are all reversed by APETx2.Conclusion and ImplicationsThese results support the development of specific ASIC3 or combined ASIC1/3 blockers for migraine‐related pain and point to a potential role for ASIC‐dependent NO‐mediated attack triggering. This has key implications for migraine, given the major unmet need for novel therapeutic targets.
Factors associated with initiation of community‐based therapy for emerging adults with mood and anxiety disorders
Authors: Saunders Hailey , Osuch Elizabeth , Anderson Kelly , Martin Janet , Kunnilathu Abraham , John‐Baptiste Ava 
Published: January 2020

AbstractAimThe First Episode Mood and Anxiety Program (FEMAP) is a community‐based early intervention program that has been shown to improve health outcomes for emerging adults (EAs) with mood and anxiety disorders. However, not all EAs who are admitted to the program initiate treatment. Our aim was to identify factors that distinguish those who initiated treatment from those who did not.MethodsFEMAP administered questionnaires to EAs upon first contact with the program, collecting information on a range of socioeconomic, patient and condition‐related factors. We compared EAs who initiated treatment in the program (n = 318, 87.4%) to those who did not (n = 46, 12.6%). To examine factors associated with treatment initiation, we specified a parsimonious logistic regression model, using the method of purposeful selection to choose from a range of candidate variables.ResultsAnxiety Sensitivity Index ‐ Revised (ASI‐R), binge drinking and cannabis use were included in the final logistic regression model. Each one‐point increment in the ASI‐R score was associated with a 1% increase in the odds of treatment initiation (OR = 1.014; 95% CI [1.003, 1.026]). No other variable was significantly associated with treatment initiation.ConclusionsOur study provides insight on the differences between EAs with mood and anxiety disorders who initiated targeted treatment services and those who did not. Anxiety sensitivity was significantly associated with treatment initiation at FEMAP. Our findings suggest that it may be anxiety sensitivity, rather than depression or functional impairment per se that drive treatment initiation among EAs.
From silos to solutions: How one district is building a culture of collaboration and learning between school principals and central office leaders
Authors: King Smith Angela , Watkins Karen E. , Han Seung‐Hyun 
Published: January 2020

AbstractLarge urban school districts often struggle with hierarchy, fragmented processes and cultures whereby organisational silos between school and central office staff serve as a major barrier for reaching the desired strategic goals of improving student achievement. In addition, school district staff are undergoing change requiring leaders and staff in these organisations to adapt and build their capacity to support changing demands. However, little attention is paid to how to build the necessary systems and learning environments to support capacity building and leader development. This article reports on an action research study using mixed methods that reveal how central office leaders and school principals break down organisational silos to build a culture of learning using learning organisation principles. Using the Dimensions of the Learning Organization Questionnaire (DLOQ) short form, participants completed questions related to the school district's capacity for learning. Data from the survey and interviews outline key findings that illustrate how the learning organisation framework served as a model to create the organisational conditions and culture to enable support and cooperation for learning, capacity, change and improvement to occur. This action research case study is a look at one organisation's approach to addressing a gap in learning organisation research in the area of learning organisation interventions. In addition, it extends current research on schools as learning organisations with an example of data‐informed change in one large school district in the southern United States.
Multiple decades of stocking has resulted in limited hatchery introgression in wild brook trout (Salvelinus fontinalis) populations of Nova Scotia
Authors: Lehnert Sarah J. , Baillie Shauna M. , MacMillan John , Paterson Ian G. , Buhariwalla Colin F. , Bradbury Ian R. , Bentzen Paul 
Published: January 2020

AbstractMany populations of freshwater fishes are threatened with losses, and increasingly, the release of hatchery individuals is one strategy being implemented to support wild populations. However, stocking of hatchery individuals may pose long‐term threats to wild populations, particularly if genetic interactions occur between wild and hatchery individuals. One highly prized sport fish that has been heavily stocked throughout its range is the brook trout (Salvelinus fontinalis). In Nova Scotia, Canada, hatchery brook trout have been stocked since the early 1900s, and despite continued stocking efforts, populations have suffered declines in recent decades. Before this study, the genetic structure of brook trout populations in the province was unknown; however, given the potential negative consequences associated with hatchery stocking, it is possible that hatchery programs have adversely affected the genetic integrity of wild populations. To assess the influence of hatchery supplementation on wild populations, we genotyped wild brook trout from 12 river systems and hatchery brook trout from two major hatcheries using 100 microsatellite loci. Genetic analyses of wild trout revealed extensive population genetic structure among and within river systems and significant isolation‐by‐distance. Hatchery stocks were genetically distinct from wild populations, and most populations showed limited to no evidence of hatchery introgression (<5% hatchery ancestry). Only a single location had a substantial number of hatchery‐derived trout and was located in the only river where a local strain is used for supplementation. The amount of hatchery stocking within a watershed did not influence the level of hatchery introgression. Neutral genetic structure of wild populations was influenced by geography with some influence of climate and stocking indices. Overall, our study suggests that long‐term stocking has not significantly affected the genetic integrity of wild trout populations, highlighting the variable outcomes of stocking and the need to evaluate the consequences on a case‐by‐case basis.
The effects of mesoscale oceanographic structures and ambient conditions on the catch of albacore tuna in the South Pacific longline fishery
Authors: Zhou Cheng , He Pingguo , Xu Liuxiong , Bach Pascal , Wang Xuefang , Wan Rong , Tang Hao , Zhang Yu 
Published: January 2020

AbstractAlbacore tuna (Thunnus alalunga) exhibit patchy concentrations associated with biological process at a wide range of spatial scales, resulting in variations in their catchability by fishing gears. Here, we investigated the association of catch variation for pelagic longlines in the South Pacific Ocean with oceanographic mesoscale structures (in horizontal dimension) and ambient conditions (in vertical dimension). The distribution of albacore tuna as indicated by catch per unit effort (CPUE) of longlines was significantly related to the presence of mesoscale structures, with higher CPUE found at locations closer to thermal fronts and with greater gradient magnitudes, as well as areas marked by peripheral contour line of the anticyclone indicated by Sea Surface Height Anomalies ~0.05 m. Surface mesoscale current velocity had the negative effect on the catch, probably as a result of decreased catchability by shoaling the hook depth. Vertical distribution of albacore in the survey region of South Pacific Ocean was hardly restricted by ambient temperature and oxygen concentration, though effect of ambient temperature was relevant and showed a negatively linear correlation with CPUE at the range of 20–24°C. On the contrary, albacore distribution was evidently dominated by the water depth and showed strong preference on water depth of 200 m, which was likely a representative feeding layer. The presence of prey resources and their accessibility by albacore revealed by mesoscale structures in the biological and physical processes, and catchability determined by the location of the baited hooks comprehensively contribute to the variability of catch.
Renal dysplasia masquerading as a renal mass in a child on hemodialysis
Authors: Acharya Ratna , Bowser Meghan , Choudhari Pooja , Upadhyay Kiran K. 
Published: January 2020

AbstractPathological renal mass is uncommon in children. It is important to differentiate this from a benign mass or mass‐like lesion (pseudomass) for proper management. Renal dysplasia is a common finding in patients with end stage renal disease and can mimic a renal mass. Here, we report a 16‐year‐old girl on hemodialysis who was found to have a nodular right renal mass in the sonogram. Magnetic resonance imaging confirmed the nodular mass. She underwent right nephrectomy and the histopathology revealed features of renal dysplasia and end stage kidney disease without any evidence of malignancy. No further treatments were necessary. This case demonstrates that a nodular renal mass in dialysis patients does not always mean malignancy and could be a pseudomass from severe renal dysplasia. Since a sonogram may not be able to clearly define the etiology of solid mass in these patients, further evaluations including a renal histology and/or other imaging modalities are often necessary.
Electrogram‐only guided approach to His bundle pacing with minimal fluoroscopy: A single‐center experience
Authors: Zanon Francesco , Marcantoni Lina , Zuin Marco , Pastore Gianni , Baracca Enrico , Tiribello Antonella , Raffagnato Paola , Boaretto Graziano , Roncon Loris , Vijayaraman Pugazhendhi 
Published: January 2020

AbstractIntroductionHis bundle pacing (HBP) is the most physiological pacing. The standard technique based on fluoroscopic approach might be challenging and fluoro consuming. Targeting the His guided exclusively by the electrical signals could enable a precise lead implant, thus reducing fluoroscopy time (FT) and X‐ray dose, desirable both for patients and operators. The aim of the study is to evaluate the feasibility, efficacy, and safety both acutely and at 30 days of the electrogram (EGM)‐guided HBP with minimal or no fluoroscopy.Methods and ResultsBetween October and December 2018, 41 consecutive patients underwent EGM‐guided HBP. Successful HBP was obtained in 39 (95%) patients, (30 males, 78 ± 10 years). Selective HBP (S‐HBP) was achieved in 23 (59%), nonselective HBP (NS‐HBP) in 16 (41%) patients. The final HBP lead position was reached in 31 (79.4%) patients without fluoroscopy, only guided by electrical signals. In eight patients a minimal fluoroscopy (mean, 8 seconds) has been required. The sheath's cutting and the slack of the lead were routinely performed under fluoroscopy. No difference was observed in FT for HBP lead placement in S‐HBP and NS‐HBP (mean, 8.1 ± 25 vs 7.5 ± 20 seconds, P = .8; median value 0 vs 0 seconds). No differences were observed in FT for the entire procedure, total dose area product and total procedural time in S‐HBP and NS‐HBP. Lead dislodgement occurred in one (2.6%) patient 1 day after the procedure.ConclusionsHBP could be performed safely and efficiently using the EGMs, with minimal or no fluoroscopy. Fluoroscopy was required during sheath removal and atrial lead placement.
Metformin upregulates mitophagy in patients with T2DM: A randomized placebo‐controlled study
Authors: Bhansali Shipra , Bhansali Anil , Dutta Pinaki , Walia Rama , Dhawan Veena 
Published: January 2020

AbstractImpaired mitochondrial autophagy (mitophagy) and NLRP3 inflammasome activation have been incriminated in the pathogenesis of T2DM. Metformin besides being an insulin sensitizer also induces autophagy; however, its effect on mitophagy and NLRP3 activation in patients with T2DM still remains elusive. Forty‐five drug‐naïve T2DM patients with HbA1C 7%‐9% (53‐75 mmol/mol) were randomly assigned to receive either metformin, voglibose, or placebo for 3 months, and were also recommended for lifestyle intervention programme (n = 15 each). Mitochondrial oxidative stress (MOS) parameters, qPCR and immunoblotting of mitophagy‐related markers (PINK1, PARKIN, MFN2, NIX, LC3‐II, LAMP2), p‐AMPKα (T172), and NLRP3 proteins, as well as transmission electron microscopy (TEM) for assessing mitochondrial morphology were performed in the mononuclear cells of study patients. Both metformin and voglibose showed a similar efficacy towards the reduction in HbA1c and MOS indices. However, multivariate ANCOVA divulged that mRNA and protein expression of mitophagy markers, NLRP3 and p‐AMPKα (T172), were significantly increased only with metformin therapy. Moreover, PINK1 expression displayed a significant positive association with HOMA‐β indices, and TEM studies further confirmed reduced distortions in mitochondrial morphology in the metformin group only. Our observations underscore that metformin upregulates mitophagy and subsequently ameliorates the altered mitochondrial morphology and function, independent of its glucose‐lowering effect. Further, restoration of normal mitochondrial phenotype may improve cellular function, including β‐cells, which may prevent further worsening of hyperglycaemia in patients with T2DM.