bluuebunny/crossref_metadata_2025_split huggingface dataset:
Differential Transcriptional Regulation of the NANOG Gene in Chicken Primordial Germ Cells and Embryonic Stem Cells
Authors: Choi Hee Jung , Jin So Dam , Rengaraj Deivendran , Kim Jin Hwa , Pain Bertrand , Han Jae Yong 
Published: September 2020

Abstract
BackgroundNANOG is a core transcription factor (TF) in embryonic stem cells (ESCs) and primordial germ cells (PGCs). Regulation of the NANOG gene by TFs, epigenetic factors, and autoregulatory factors is well characterized in ESCs, and transcriptional regulation of NANOG is well established in these cells. Although NANOG plays a key role in germ cells, the molecular mechanism underlying its transcriptional regulation in PGCs has not been studied. Therefore, we investigated the mechanism that regulates transcription of the chicken NANOG (cNANOG) gene in PGCs and ESCs. ResultsWe first identified the transcription start site of cNANOG by 5’-rapid amplification of cDNA ends PCR analysis. Then, we measured the promoter activity of various 5’ flanking regions of cNANOG in chicken PGCs and ESCs using the luciferase reporter assay. cNANOG expression required transcriptional cis-regulatory elements, which were positively regulated by POU5F3 (OCT4) and SOX2 and negatively regulated by TP53 in PGCs. The proximal region of the cNANOG promoter contains a positive cis-regulatory element (CCAAT/enhancer-binding protein (CEBP)-binding site) in ESCs. Furthermore, small interfering RNA-mediated knockdown demonstrated that POU5F3, SOX2, and CEBP played a role in cell type-specific transcription of cNANOG.ConclusionsWe show for the first time that different cis-regulatory elements control transcription of cNANOG in a cell type-specific manner. This finding might help to elucidate the mechanism that regulates cNANOG expression in PGCs and ESCs.
A Self-administered Version of the Functioning Assessment Short Test for Use in Population-based Studies: A Pilot Study
Authors: Riegler Christoph , Wiedmann Silke , Rücker Viktoria , Teismann Henning , Berger Klaus , Störk Stefan , Vieta Eduard , Faller Hermann , Baune Bernhard T , Heuschmann Peter U 
Published: September 2020

Background:
The Functioning Assessment Short Test (FAST) is an interviewer-administered scale assessing functional impairment originally developed for psychiatric patients.

Objectives:
To adapt the FAST for the general population, we developed a self-administered version of the scale and assessed its properties in a pilot study.

Methods:
The original FAST scale was translated into German via forward and backward translation. Afterwards, we adjusted the scale for self-administered application and inquired participants from two ongoing studies in Germany, ‘STAAB’ (Würzburg) and ‘BiDirect’ (Münster), both recruiting subjects from the general population across a wide age range (STAAB: 30-79 years, BiDirect: 35-65 years). To assess reliability, agreement of self-assessment with proxy-assessment by partners was measured via intraclass correlation coefficient (ICC) over the FAST score. Construct validity was estimated by conducting correlations with validated scales of depression (PHQ-9), anxiety (GAD-7), and health-related quality of life (SF-12) and regression analyses using these scales besides potentially disabling comorbidities (e.g. Chronic Back Pain (CBP)).

Results:
Participants (n=54) had a median age of 57.0 years (quartiles: 49.8, 65.3), 46.3% were female. Reliability was moderate: ICC 0.50 (95% CI 0.46-0.54). The FAST score significantly correlated with PHQ-9, GAD-7, and the mental sub-scale of SF-12. In univariable linear regression, all three scales and chronic back pain explained variance of the FAST score. In multivariable analysis, only CBP and the SF-12 remained significant predictors.

Conclusion:
The German self-administered version of the FAST yielded moderate psychometric properties in this pilot study, indicating its applicability to assess functional impairment in the general population.
Protective Effect of Isothiocyanates from Cruciferous Vegetables on Breast Cancer: Epidemiological and Preclinical Perspectives
Authors: Ngo Suong N.T. , Williams Desmond B. 
Published: September 2020

Background:The effect of cruciferous vegetable intake on breast cancer survival is controversial at present. Glucosinolates are the naturally occurring constituents found across the cruciferous vegetables. Isothiocyanates are produced from the hydrolysis of glucosinolates and this reaction is catalysed by the plant-derived enzyme myrosinase. The main isothiocyanates (ITCs) from cruciferous vegetables are sulforaphane, benzyl ITC, and phenethyl ITC, which had been intensively investigated over the last decade for their antibreast cancer effects.Objective:The aim of this article is to systematically review the evidence from all types of studies, which examined the protective effect of cruciferous vegetables and/or their isothiocyanate constituents on breast cancer.Methods:A systematic review was conducted in Pubmed, EMBASE, and the Cochrane Library from inception to 27 April 2020. Peerreviewed studies of all types (in vitro studies, animal studies, and human studies) were selected.Results:The systematic literature search identified 16 human studies, 4 animal studies, and 65 in vitro studies. The effect of cruciferous vegetables and/or their ITCs intake on breast cancer survival was found to be controversial and varied greatly across human studies. Most of these trials were observational studies conducted in specific regions, mainly in the US and China. Substantial evidence from in vitro and animal studies was obtained, which strongly supported the protective effect of sulforaphane and other ITCs against breast cancer. Evidence from in vitro studies showed that sulforaphane and other ITCs reduced cancer cell viability and proliferation via multiple mechanisms and pathways. Isothiocyanates inhibited cell cycle, angiogenesis and epithelial mesenchymal transition, as well as induced apoptosis and altered the expression of phase II carcinogen detoxifying enzymes. These are the essential pathways that promote the growth and metastasis of breast cancer. Noticeably, benzyl ITC showed a significant inhibitory effect on breast cancer stem cells, a new dimension of chemo-resistance in breast cancer treatment. Sulforaphane and other ITCs displayed anti-breast cancer effects at variable range of concentrations and benzyl isothiocyanate appeared to have a relatively lower inhibitory concentration IC50. The mechanisms underlying the cancer protective effect of sulforaphane and other ITCs have also been highlighted in this article.Conclusion:Current preclinical evidence strongly supports the role of sulforaphane and other ITCs as potential therapeutic agents for breast cancer, either as adjunct therapy or combined therapy with current anti-breast cancer drugs, with sulforaphane appeared to display the greatest potential.
Assessing the Dynamic Behaviour of Midrise Frame Structures Sitting on Silty Sandy Soil
Authors: Ismail Sahar , Kaddah Fouad , Raphael Wassim 
Published: September 2020

Background:Midrise 5 to 15 storeys frame structures sitting on soft soils are susceptible to damage induced by seismic events. The level of damage is related to the interaction between the structure, foundation and soil called Soil Structure Interaction (SSI). If the level of ground acceleration is low, the wave gets amplified putting the structure at risk of collapse.Objective and Methods:Concerns about SSI have motivated several researchers to investigate the seismic behaviour of structures rested on cohesive and cohesionless soils. The objective of the work presented in this paper is to evaluate the effects of several parameters on the seismic soil structure interaction behaviour of midrise structures sitting on silty sandy soil. Using ABAQUS, reliable 3D models of 5 to 15 storeys midrise concrete frame structures rested on raft foundation were built. The effects of the structure’s number of storeys, raft size and thickness were explored for different column sizes. Fixed-based structures which capture the model adopted in seismic codes and flexible-based structures were hit at the bottom by El-Centro (1940) and Northridge (1994) earthquakes.Results and Conclusion:The results, presented in terms of storey lateral deflection, inter-storey drift, shear force, foundation rocking and response spectrum showed the important contribution of SSI effects on the behaviour of the midrise structures. The model analyses indicated that column size strongly affects the behaviour of flexible structures. LetNbe the structure number of storeys andCthe column size. The results showed that in terms of storey lateral deflection and levelling shear force, for column sizes C 0.5 X 0.5 m, SSI was detrimental to structures with 10 ≤N≤ 15 and beneficial to structures with 5 ≤N<10. Increasing the column size to C 0.5 X 1 m showed that SSI became detrimental for structures with 10 <N≤ 15 under El-Centro (1940) and for structures with 7≤N≤ 15 under Northridge (1994), and beneficial for structures with 5 ≤N≤ 10 under El-Centro (1940) and for structures with 5 ≤N< 7 under Northridge (1994). The FE results showed that even though base shear increased with raft size, lateral deflections were amplified for C 0.5 X 0.5 m S15 structures and attenuated for C 0.5 X 1 m S15 structures. However, the seismic response of S15 structures was slightly affected by the variation in raft thickness under both column sizes. Finally, the paper includes a discussion and evaluation of the contribution of inertial and kinematic effects, including soil types used on the simulated numerical models’ seismic responses.
Real-World Evidence of User Engagement With Mobile Health for Diabetes Management: Longitudinal Observational Study
Authors: Böhm Anna-Katharina , Jensen Morten Lind , Sørensen Mads Reinholdt , Stargardt Tom 
Published: September 2020

Background
Patient support apps have risen in popularity and provide novel opportunities for self-management of diabetes. Such apps offer patients to play an active role in monitoring their condition, thereby increasing their own treatment responsibility. Although many health apps require active user engagement to be effective, there is little evidence exploring engagement with mobile health (mHealth).

Objective
This study aims to analyze the extent to which users engage with mHealth for diabetes and identify patient characteristics that are associated with engagement.

Methods
The analysis is based on real-world data obtained by Novo Nordisk’s Cornerstones4Care Powered by Glooko diabetes support app. User engagement was assessed as the number of active days and using measures expressing the persistence, longevity, and regularity of interaction within the first 180 days of use. Beta regressions were estimated to assess the associations between user characteristics and engagement outcomes for each module of the app.

Results
A total of 9051 individuals initiated use after registration and could be observed for 180 days. Among these, 55.39% (5013/9051) used the app for one specific purpose. The average user activity ratio varied from 0.05 (medication and food) to 0.55 (continuous glucose monitoring), depending on the module of the app. Average user engagement was lower if modules required manual data entries, although the initial uptake was higher for these modules. Regression analyses further revealed that although more women used the app (2075/3649, 56.86%), they engaged significantly less with it. Older people and users who were recently diagnosed tended to use the app more actively.

Conclusions
Strategies to increase or sustain the use of apps and availability of health data may target the mode of data collection and content design and should take into account privacy concerns of the users at the same time. Users’ engagement was determined by various user characteristics, indicating that particular patient groups should be targeted or assisted when integrating apps into the self-management of their disease.
Pamer Kemewahan: Kajian Teori Konsumsi Thorstein Veblen
Authors: Bakti Indra Setia , Anismar Anismar , Amin Khairul 
Published: September 2020

This article aims to discuss Thorstein Veblen's perspective about the behavior of waste or excessive consumption by the leisure class. This article uses the library research to understanding the perspective of Veblen's theory of consumption. We review Veblen's work, The Theory of the Leisure Class, as the main note complemented by relevant books and journals to support this study. The leisure class in this regard act deliberately to display their wealth. The newly rich group flaunted the luxury of their life with a motive to accommodate their desire for social respect and social status. The leisure class realizes their social actions through conspicuous leisure time consumption and conspicuous consumption of goods characterized by imitative and emulative behavior among the actors involved in it. The conspicuous consumption behavior produces élite taste which in turn has a social impact that affects the class behavior of the lower strata.AbstrakArtikel ini bertujuan untuk mendiskusikan sudut pandang Thorstein Veblen dalam melihat perilaku konsumsi berlebihan yang dilakukan oleh kelas sosial tertentu dalam masyarakat. Studi ini menggunakan metode kajian pustaka dalam memahami perspektif teori konsumsi Veblen. Data dalam artikel ini bersumber dari karya-larya Veblen sendiri, The Theory of the Leisure Class, serta buku-buku dan jurnal-jurnal yang relevan dalam mendukung artikel ini. Perilaku ini rupanya lahir dari sebuah konteks sosial dimana kelompok orang kaya baru mencoba mengakomodasi hasrat mereka akan penghargaan sosial dan status sosial. Hal ini diwujudkan melalui konsumsi waktu luang mencolok dan konsumsi barang mencolok yang ditandai dengan perilaku imitatif dan emulatif diantara aktor-aktor yang terlibat di dalamnya. Perilaku konsumsi mencolok menghasilkan selera elite yang selanjutnya meluas dan berdampak secara sosial dimana mempengaruhi perilaku kelas dari strata yang lebih rendah
Pembentukan Manhaj Jamaah Dalam Pengembangan Masyarakat Islam
Authors: Dianto, S.Sos.I., M.Kom.I Icol 
Published: September 2020

AbstractIslam as the religion of rahmatanlil'alamin means that Islam is universal and flexible to all aspects of life, including the life of mankind. Da'wah of Islam touches on the development of the resources of the Muslim community and the global community in humanizing humans, achieving a decent standard of living materially and immaterial (hasanah fid-dunya wal akhirat). However, the availability of potential natural resources has not been maximally utilized because of the low capacity and weak work ethic. Therefore, the establishment of Manhaj Jama'ah in development and empowerment activities is important to implement. Development that is centered on pilgrims can have a broader impact in efforts to build people's welfare and can build "peer networks" in community development activities.This paper is not an applicative study of a study but contains theoretical-academic exposures, which explain the efforts to develop Islamic society in congregation. In this article, it was explained the reason for the need for community development activities carried out in congregation, what strengths the pilgrims have and the stages of forming strong and independent jama'ah.Keywords: Manhaj, Jamaah, Community Development. AbstrakIslam sebagai agama rahmatanlil’alamin bermakna bahwa Islam itu universal dan fleksibel terhadap semua aspek kehidupan makhluk, termasuk kehidupan umat manusia. Dakwah Islam menyentuh pembangunan sumber daya komunitas muslim dan masyarakat global dalam memanusiakan manusia, mencapai taraf hidup layak secara material dan immaterial (hasanah fid-dunya wal akhirat). Akan tetapi, ketersediaan potensi sumberdaya alam itu belum maksimal dimanfaatkan karena rendahnya kemampuan dan lemahnya etos kerja. Oleh karena itu, pembentukan manhaj jama’ah dalam kegiatan pengembangan dan pemberdayaan menjadi penting untuk dilaksanakan. Pengembangan yang dipusatkan pada jamaah dapat memberikan dampak yang lebih luas dalam upaya membangun kesejahteraan umat dan dapat membangun “jaringan sebaya” dalam kegiatan pengembangan masyarakat.Paper ini bukanlah kajian aplikatif dari sebuah penelitian melainkan berisi paparan teoritis-akademis, yang menjelaskan upaya pengembangan masyarakat Islam itu dilakukan secara berjamaah. Dalam artikel ini dijelaskan alasan perlunya kegiatan pengembangan masyarakat itu dilakukan secara berjamaah, kekuatan apa yang dimiliki oleh jamaah dan tahapan membentuk jama’ah yang kuat dan mandiri.Kata Kunci: Manhaj, Jamaah, Pengembangan Masyarakat.
PEMBERDAYAAN EKONOMI PEREMPUAN MELALUI HOME INDUSTRY BATIK DI DESA SENDANG DUWUR KECAMATAN PACIRAN KABUPATEN LAMONGAN
Authors: Muhtadi Muhtadi 
Published: September 2020

Abtract In this paper, want to see how the empowerment process is done and what the results can be obtained by people who are members of the home industry batik. Thus, the method used in this paper is a qualitative method, namely observations and interviews in order to see how far the process done by batik home industry and see the results obtained by its members. The theory used is the theory of empowerment stages proposed by Sumodiningrat and theory of empowerment indicators proposed by Edi Suharto. The results showed that the empowerment process conducted batik home industry in accordance with the three stages of empowerment stage of penyauaran, transformation stage and stage of intellectual improvement. While the results obtained by the community from the batik home industry use only 3 of the 8 indicators of empowerment proposed by Edi Suharto. Home industry batik can be said to have succeeded in empowering society around home industry location. Successfully transforming unproductive societies into productive ones and having income that is not only used to meet the needs of life, and can also be invested to be used in the future. Abstrak Dalam penulisan ini, ingin melihat bagaimana proses pemberdayaan yang dilakukan serta apa saja hasil yang dapat diperoleh masyarakat yang tergabung dalam home industry batik.Dengan demikian, metode yang digunakan dalam penulisan ini adalah metode kualitatif, yaitu pengamatan dan wawancara guna untuk melihat sejauh mana proses yang dilakukan oleh home industry batik serta melihat hasil yang didapat oleh anggotanya. Teori yang digunakan adalah teori tahapan pemberdayaan yang dikemukakan oleh Sumodiningrat dan teori indikator pemberdayaan yang dikemukakan oleh Edi Suharto.Hasil penelitian menunjukan bahwa proses pemberdayaan yang dilakukan home industry batik sesuai dengan tiga tahapan pemberdayaan yaitu tahap penyadaaran, tahap transformasi dan tahap peningkatan intelektualitas. Sedangkan hasil yang diperoleh oleh masyarakat dari home industry batik hanya menggunakan 3 dari 8 indikator pemberdayaan yang dikemukakan oleh Edi Suharto.Home industry batik dapat dikatakan sudah berhasil memberdayakan masyarakat disekitar lokasi home industry. Berhasil merubah masyarakat yang tidak produktif menjadi produktif serta memiliki penghasilan yang tidak hanya dipergunakan untuk memenuhi kebutuhan hidup, dan juga bisa diinvestasikan untuk dipergunakan dimasa yang akan mendatang.
Kepemimpinan Komunitas: Studi Pemahaman Hadis Kepemimpinan di Tangan Bani Quraisy
Authors: Dianto Icol 
Published: September 2020

This article discusses traditions which state that the affairs of leadership are held by the Bani Quraisy contained in the authentic Hadith books. By using the study of literature through the approach of the method of understanding the hadith and sociological approach to the source of data in the form of classic hadith books in the books of Shahih Bukhari, Shahih Muslim, Musnad Imam Ahmad and Musnad Abu Daud, books and scientific articles, obtained the results of studies that scholars agreed on the validity of the traditions about the Caliphate (leadership) in the hands of Bani Quraisy, but the scholars differed in interpreting the hadith, between afdlaliyyah (the conditions of virtue) and in'iqad (mutlaq terms). In contemporary society with a sociological approach to the authentic hadiths, an understanding is obtained that the leader must have a sense of justice, compassion, keep promises, uphold religion and invite service and prevent disobedience. Leaders who have these requirements must be obeyed by their people (their community).Keywords: Leadership Hadith, Caliphate, Bani Quraisy, Community Leader. Artikel ini membahas tentang hadis-hadis yang menyatakan bahwa urusan kepemimpinan dipegang oleh Bani Quraisy yang terdapat dalam kitab-kitab hadis shahih. Dengan menggunakan kajian studi kepustakaan melalui pendekatan metode pemahaman hadis dan pendekatan sosiologis terhadap sumber data berupa kitab-kitab hadis klasik dalam kitab Shahih Bukhari, Shahih Muslim, Musnad Imam Ahmad dan Musnad Abu Daud, buku-buku dan artikel ilmiah, didapatkan hasil kajian bahwa ulama sepakat atas keshahihan hadis-hadis tentang kekhalifahan (kepemimpinan) di tangan Quraisy, namun para ulama itu berbeda pendapat dalam menginterpretasikan hadis, antara afdlaliyyah (syarat keutamaan) dengan in’iqad (syarat mutlaq).Pada masyarakat kontemporer dengan pendekatan sosiologis terhadap hadis-hadis shahih tersebut didapatkan pemahaman bahwa pemimpin itu harus memiliki rasa keadilan, berkasih sayang, menepati janji, menegakkan agama dan mengajak kepada kabajikan serta mencegah kemaksiatan. Pemimpin yang memiliki persyaratkan itu wajib ditaati oleh rakyatnya (komunitasnya).Katakunci: Hadis Kepemimpinan, Kekhalifahan, Suku Quraisy, Pemimpin Komunitas.
Development and Validation of a HPLC Method of Quantification of Caffeine and EGCG in Green Tea (Camellia sinensis L.) Extract
Authors: Trong Bien Tra , Thi Lan Phuong Bui 
Published: September 2020

This study investigates a simple high performance liquid chromatography (HPLC) in an isocratic eluent manner for quantification of caffeine and EGCG in green tea extract. The study separation system contained a C18-RP column (250 × 4.6 mm, 5 µm), a mobile phase of methanol/phosphoric acid/water (20/0.1/79.9, v/v) and a DAD detector at 280 nm. This system proves convenient for rapid routine analysis of caffeine and EGCG in green tea extract with good repeatability and accuracy.
Keywords
HPLC, caffeine, EGCG, green tea, extract.
References
[1] A. Chowdhury, J. Sarkar, T. Chakraborti, P.K. Pramanik, S. Chakraborti, Protective role of epigallocatechin-3-gallate in health and disease: A perspective, Biomedicine & Pharmacotherapy 78 (2016) 50-59. https://doi.org/10.1016/j.biopha.2015.12.013.[2] Y. Hara, Tea catechins and their applications as supplements and pharmaceutics, Pharmacological Research 64(2) (2011) 100-104. https://doi.org/10.1016/j.phrs.2011.03.018.[3] X. Jun, S. Zhao, B. Lu, R. Zhang, Y. Li, D. Shen, G. Zhou, Separation of major catechins from green tea by ultrahigh pressure extraction, International Journal of Pharmaceutics 386(1-2) (2010) 229-231. https://doi.org/10.1016/j.ijpharm.2009.10.035.[4] Z. Bedrood, M. Rameshrad, H. Hosseinzadeh, Toxicological effects of Camellia sinensis (green tea): A review, Phytotherapy Research 32(7) (2018) 1163-1180. https://doi.org/10.1002/ptr.6063.[5] J. Michael Glade, Caffeine-Not just a stimulant, Nutrition 26(10) (2010) 932-938. https://doi.org/10.1016/j.nut.2010.08.004.[6] MediGene AG. Press Release., MediGene AG Obtains Approval of Polyphenon®E Ointment in the USA, 2006.[7] Q.V. Vuong, V. Nguyen, J.B. Golding, P.D. Roach, The content of bioactive constituents as a quality index for Vietnamese teas, International Food Research Journal 18(1) (2011) 329-336.[8] Tran Trong Bien, Method for caffeine removal and catechins enrichment from green tea leaf, Master thesis, Hanoi University of Pharmacy, 2019 (In Vietnamese).[9] J. Joseph Dalluge, C. Bryant Nelson, Determination of tea catechins, Journal of Chromatography A 881 (2000) 411–424. https://doi.org/10.1016/S0021-9673(00)00062-5.[10] T. Shiono, et al., Selective decaffeination of tea extracts by montmorillonite, Journal of Food Engineering 200 (2017) 13-21. https://doi.org/10.1016/j.jfoodeng.2016.12.015.