bluuebunny/crossref_metadata_2025_split huggingface dataset:
Relative Expression of Mouse Udp-glucuronosyl Transferase 2b1 Gene in the Livers, Kidneys, and Hearts: The Influence of Nonsteroidal Anti-inflammatory Drug Treatment
Authors: Jarrar Yazun , Jarrar Qais , Abu-Shalhoob Mohammad , abed Abdulqader , Sha'ban Esra'a 
Published: November 2019

Background:
Mouse Udp-glucuronosyl Transferase (UGT) 2b1 is equivalent to the human UGT2B7
enzyme, which is a phase II drug-metabolising enzyme and plays a major role in the metabolism of xenobiotic and
endogenous compounds. This study aimed to find the relative expression of the mouse ugt2b1 gene in the liver, kidney,
and heart organs and the influence of Nonsteroidal Anti-inflammatory Drug (NSAID) administration.

Methods:
Thirty-five Blab/c mice were divided into 5 groups and treated with different commonly-used NSAIDs;
diclofenac, ibuprofen, meloxicam, and mefenamic acid for 14 days. The livers, kidneys, and hearts were isolated,
while the expression of ugt2b1 gene was analysed with a quantitative real-time polymerase chain reaction technique.

Results:
It was found that the ugt2b1 gene is highly expressed in the liver, and then in the heart and the kidneys.
NSAIDs significantly upregulated (ANOVA, p < 0.05) the expression of ugt2b1 in the heart, while they downregulated
its expression (ANOVA, p < 0.05) in the liver and kidneys. The level of NSAIDs’ effect on ugt2b1 gene expression
was strongly correlated (Spearman’s Rho correlation, p < 0.05) with NSAID’s lipophilicity in the liver and its
elimination half-life in the heart.

Conclusion:
This study concluded that the mouse ugt2b1 gene was mainly expressed in the liver, as 14-day administration
of different NSAIDs caused alterations in the expression of this gene, which may influence the metabolism
of xenobiotic and endogenous compounds.
Multi-element Quantification in Parenteral Nutrition Mixture by MP-AES and Comparison with ICP-AES
Authors: Merienne Camille , Marchand Chloe , Filali Samira , Salmon Damien , Vernoux Karine , Pivot Christine , Pirot Fabrice 
Published: November 2019

Background:
Individualized parenteral nutrition requires tailor-made preparation in hospital
pharmacy followed by suitable quality control, including electrolytic content determination within
complex and variable admixtures, containing up to 50 ingredients.

Materials and Methods:
An analytical tool has been developed to simultaneously quantify four major
electrolytes in parenteral in parenteral nutrition mixtures (i.e., sodium, potassium, magnesium and calcium)
using microwave plasma atomic emission spectroscopy (MP-AES). A comparison with reference
inducted coupled plasma atomic emission spectroscopy (ICP-AES) was conducted. Calibration
standard solutions ranged between 0.2 to 5.0 mg.L-1 for sodium, potassium and calcium and 0.1 to 2.5
mg.L-1 for magnesium. Wavelengths used were respectively 422.6 nm, 517.2 nm, 766.4 nm and 589.5
nm for calcium, magnesium, potassium and sodium, respectively. Accuracy profiles were used for the
validation protocol.

Results and Conclusion:
The analytical protocol using MP-AES demonstrated high throughput and
good sensitivity, thus enabling efficient quality control of manufactured individualized parenteral nutrition
mixtures.
miR-21 and miR29-a: Potential Molecular Biomarkers for HPV Genotypes and Cervical Cancer Detection
Authors: Zamani Sara , Hosseini Seyed Masoud , Sohrabi Amir 
Published: November 2019

Background and objective:
This study aimed to assess miR21 and miR-29a diagnostic
usefulness for HPV infection and Cervical Cancer (CC) detection.

Methods:
miRs extraction has been performed on HPV-positive, CC and negative control of 43, 50
and 46 individuals, respectively. The expression level of miRs has been analyzed using Real-Time
RT-PCR.

Results:
There was a correlation between the expression of miRs and the Receiver Operating Characteristic
(ROC) analysis in CC and HPV-positive patients.

Conclusion:
The correlation between expressions of miRs is notable. ROC curve analysis could be
applied in molecular diagnostic exposure for CC related to HPV infections.
Data Pattern Of Computer Maintenance Management System With Eclat Algorithm
Authors: Sukmana Farid , Rozi Fahrur 
Published: November 2019

Decision support system, basically used to help choosing some solution for stakeholde to take the best decision in manufacturer. In manufacturer company using Enterprise Resource System (ERP) that has Work Oder (WO) modul as request maintenance from user. But many of data from WO still didn’t use to help decision making and only as warehouse data about infrastructure maintenance from last time. Because that, author use that data to help technician to take decision making by using association rule as pattern processing. This is because WO has unique pattern that has problem (p), symptom (s), and root cause (r). Previous research (Sukmana, Rozi, 2017) was proved if association rule can use to help people to take decison making, it is just involved two variable, that is symptom (s), root cause (r) and using apriori algorithm as association rule. And focussing in this research is using that three variable and eclat algorithm as association rule methode. Result of this research has purpose to take the best pattern when using eclat algorithm.
Mitigating Coordinated Call Attacks On VoIP Networks Using Hidden Markov Model
Authors: Nakorji Usman Haruna , Adedokun E A , Umoh I J , Shettima Abdullazeez 
Published: November 2019

Abstract This paper presents a 2-tier scheme for mitigating coordinated call attacks on VoIP networks. Call interaction pattern was considered using talk and salient periods in a VoIP call conversation. At the first-tier, Short Term Energy algorithm was used for call interaction feature extraction and at the second-tier Hidden Markov Model was used for caller legitimacy recognition. Data of VoIP call conversations were collated and analyzed to extract distinctive features in VoIP call interaction pattern to ascertain the legitimacy of a caller against coordinated call attacker. The performance metrics that was used are; False Error Rate (FER), Specificity, Detection Accuracy and Throughput. Several experiments were conducted to see how effective the mitigating scheme is, as the scheme acts as a proxy server to Session Initiation Protocol (SIP) server. The experiments show that; when the VoIP server is under coordinated call attack without a mitigating scheme only 15.2% of legitimate VoIP users had access to the VoIP network and out of which about half of the legitimate users had their calls dropped before completion, while with the 2-tier mitigating scheme, when the VoIP server is under coordinated call attacks over 90.3% legitimate VoIP callers had their calls through to completion
A Profile of the SAIL Databank on the UK Secure Research Platform
Authors: Jones Kerina H , Ford David Vincent , Thompson Simon , Lyons Ronan 
Published: November 2019

Background
The Secure Anonymised Information Linkage (SAIL) Databank is a national data safe haven of de‑identified datasets principally about the population of Wales, made available in anonymised form to researchers across the world. It was established to enable the vast arrays of data collected about individuals in the course of health and other public service delivery to be made available to answer important questions that could not otherwise be addressed without prohibitive effort. The SAIL Databank is the bedrock of other funded centres relying on the data for research.
Approach
SAIL is a data repository surrounded by a suite of physical, technical and procedural control measures embodying a proportionate privacy-by-design governance model, informed by public engagement, to safeguard the data and facilitate data utility. SAIL operates on the UK Secure Research Platform (SeRP), which is a customisable technology and analysis platform. Researchers access anonymised data via this secure research environment, from which results can be released following scrutiny for disclosure risk. SAIL data are being used in multiple research areas to evaluate the impact of health and social exposures and policy interventions.
Discussion
Lessons learned and their applications include: managing evolving legislative and regulatory requirements; employing multiple, tiered security mechanisms; working hard to increase analytical capacity efficiency; and developing a multi-faceted programme of public engagement. Further work includes: incorporating new data types; enabling alternative means of data access; and developing further efficiencies across our operations.
Conclusion
SAIL represents an ongoing programme of work to develop and maintain an extensive, whole population data resource for research. Its privacy-by-design model and UK SeRP technology have received international acclaim, and we continually endeavour to demonstrate trustworthiness to support data provider assurance and public acceptability in data use. We strive for further improvement and continue a mutual learning process with our contemporaries in this rapidly developing field.
Relation of Left Ventricular Diastolic Dysfunction to Serum Ferritin in Thalassemia Major
Authors: Faeq Salih Aso , M. Hamawandi Adnan 
Published: November 2019

A prospective study was conducted on 60 thalassemia major patients (33 males and 27 females ) in whom the LV function and its parameters where assessed by echocardiography in relation to serum ferritin during the period from 2nd October 2007 to 2nd February 2008, in Sulaimani pediatric teaching hospital. Patient and method: The age ranges of patients were ranged between 3 – 18 years old. The diastolic function of LV was assessed by (M-mode, D-mode, and pulse dopplar study of mitral and pulmonary vein) done. Among 60 patients seen, 25% shows normal LV diastolic function, the rest is 13% relaxation dysfunction, 27% shows Psuedonormal diastolic dysfunction and 35% shows restrictive pattern of LV diastolic dysfunction. Mean serum ferritin in normal and abnormal diastolic LV dysfunction was not so different. In addition nearly all LV parameters as ( LVEDD , LVESD , LA diameter , aortic root diameter , IVC , E ,A ,E/A ratio , A-E time , DT time , S,D,R waves ) where non significantly related to serum ferritin level , this means that P value is less than 0.05 . This means that the level of serum ferritin has no relation with the above parameters in which 75% of those parameters were already abnormal in comparison to the age of the child. Another finding shows that 25% of normal diastolic function is seen in serum levels above 1500 ng/l, in addition, 50% of diastolic dysfunction will associate serum ferritin of less than 500ng/l . P value is more than 0,05 this means that its non-significant. In conclusion, there is a non-significant correlation between serum ferritin and LV diastolic function, so serum ferritin is not a dependant indicator for myocardial iron deposition.
The Removal of Reactive Red 141 From Wastewater: A Study of Dye Adsorption Capability of Water-Stable Electrospun Polyvinyl Alcohol Nanofibers
Authors: Akduman Çigdem , Morsümbül Seniha , Kumbasar Emriye Perrin Akçakoca 
Published: November 2019

Abstract
The dye production and its use in textile and related industries resulted in discharge of dye to wastewater. Adsorption for color removal is known as equilibrium separation process, and the resultant decolorization is influenced by physicochemical factors such as adsorbent surface area. The nanofiber membranes prepared by the electrospinning method have controllable nanofiber diameter and pore size distribution (PSD) with a high surface area to volume or mass ratio. In this study, polyvinyl alcohol (PVA) nanofibrous membranes were prepared by the electrospinning method at different collection times such as 3, 5 and 10 h and heat fixated at 130, 150 and 170°C for 10 min, and then, the adsorption capability of PVA nanofiber membranes for Reactive Red 141 from aqueous solution was investigated. In order to make PVA nanofibers stable to water, the nanofibrous membranes were chemically cross-linked by a polycarboxylic acid (1,2,3,4 butanetetracarboxylic acid (BTCA)). PVA nanofibrous membranes were characterized by scanning electron microscopy, thermogravimetric analysis, swelling tests and pore size analysis. The results indicated that BTCA crosslinking improved the thermal and water stability of the nanofibrous structure but has no significant effect on the pore sizes of the membranes. Adsorption of Reactive Red 141 was studied by the batch technique, and it was observed that PVA nanofibers removed approximately >80% of the dye.
Integration of digital technologies in the field of construction in the Russian Federation
Authors: Aleksandrova Elena , Vinogradova Victoria , Tokunova Galina 
Published: November 2019

Abstract
The article presents the study that mainly focused on the changes made as a result of collaborative innovations in business relationships developed during the period of digitalisation in the construction field of the Russian Federation. It is a conceptual piece of work based on the systematic approach to the analysis, literature review and comparative analysis. The digitalisation of investment and construction projects is technologically based on the integration of solutions, such as the building information model (BIM), high-performance IT-systems, cloud platforms and the Internet-of-Things, resulting in unified and constant connectivity, specialised mobile applications, robotic equipment, unmanned vehicles, additive technologies, AR/VR services for the analysis of Big-Data, and blockchain technologies. The integration of digital technologies is a radical innovation, which highlights collaborative innovations in business relationships and makes it possible to form a united digital ecosystem that allows firms to manage, control and regulate the full lifecycle of a construction project, and then, the property in real-time. The contribution of this work to the construction field is the offered model for the creation of a digital ecosystem and the described role of the government in the model. Also, this work can be used for the integration of BIM technologies in construction companies.
Internet-based consumer co-creation experience of the new product development process
Authors: Khrystoforova Viktoria , Siemeniako Dariusz 
Published: November 2019

Abstract
The paper aims to explore consumer co-creation experience of new product development processes. Specifically, it is an attempt to determine the level of consumer engagement in an online co-creation process, identifying motives and reasons for the participation in new product development as well as understanding the types of Internet-based co-creation that are mostly preferred by consumers. The study used an online questionnaire and the CAWI method. The results of the research showed that consumers were interested in being involved in the co-creation of new product development. However, some consumers clearly expressed their reservations regarding participation because they felt lacking required knowledge.
Early postnatal hypothalamic-pituitary-adrenal axis activity and reduced insulin sensitivity in adult rats
Authors: Sadeghimahalli Forouzan , Zardooz Homeira , Golchoobian Ravieh 
Published: November 2019

Abstract

Objective. Early life stress influences the development of metabolic disorders, including functional changes in the developing of pancreas mediated hypothalamic-pituitary-adrenal (HPA) axis. In the present study, the role of an early postnatal stress on corticosterone, glucose, and insulin levels was investigated during young adulthood.

Methods. Two groups of pups were studied, including control group (pups not receiving foot shock by communication box), and early stress group (pups receiving foot shock by communication box 2 times/day for 5 consecutive days). In rats, concentration of plasma corticosterone, glucose, and insulin was detected before and after placing them into the communication box at 2 weeks of age. At 8–10 weeks of age, concentrations of plasma corticosterone, glucose, and insulin and glucose tolerance were measured in young adult rats.

Results. Our results showed that early postnatal foot shock stress increased the corticosterone, insulin, and glucose levels in the postnatal age (p<0.01) that did not last until young adult age, but it caused a significant increase in plasma glucose and insulin levels (p<0.05) following the intraperitoneal glucose tolerance test (IPGTT) in young adult rats.

Conclusions. These results suggest that impaired IPGTT in young adult rats who experienced early postnatal stress can indicate insulin resistance or reduced insulin sensitivity that make it at risk of the type 2 diabetes later in life.
Comparing the preventive effect of sodium hydrosulfide, leptin, and curcumin against L-arginine induced acute pancreatitis in rats: role of corticosterone and inducible nitric oxide synthase
Authors: Ragy Merhan M. , Ali Fatma F. , Toni Nisreen D. M. 
Published: November 2019

Abstract

Objectives. Acute pancreatitis (AP) is a life-threatening condition. Using antioxidants in AP is insufficient and conflicting. Therefore, this study compared the effect of hydrogen sulfide (H2S) donor, sodium hydrosulfide (NaHS), leptin or curcumin pretreatment on AP induced by L-arginine.

Methods. Forty adult male rats were used and classified into: 1) control; 2) AP group [each rat was intraperitoneally (i.p.) injected with 2 doses of L-arginine of 250 mg/100 g body weight (b.w.) with an interval of 1 h]; 3) NaHS+AP group (each rat was i.p. injected with 10 mg/kg b.w. of NaHS 1 h before induction of AP); 4) leptin+AP group (each rat was pretreated with 10 μg/kg b.w. of leptin 30 min before induction of AP; and 5) curcumin+AP group (in which rats were i.p. injected with 150 mg/kg b.w. of curcumin 30 min before induction of AP). Serum amylase, lipase, nitric oxide (NO), tumor necrosis factor alpha (TNF-α), and corticosterone (CORT) levels were assayed. In addition, pancreatic tissues were obtained for histopathological examination and malondialde-hyde (MDA), total antioxidant capacity (TAC), and inducible nitric oxide synthase (iNOS) levels were measured.

Results. All AP treated groups showed significant decrease in serum levels of pancreatic enzymes, NO, and TNF-α, and pancreatic MDA and iNOS levels, while TAC levels were significantly increased. NaHS caused more limitation of inflammation than leptin and curcumin by affecting iNOS. Leptin was more potent than curcumin due to the stimulatory effect of leptin on glucocorticoid release to counteract inflammation.

Conclusions. NaHS was more effective in AP amelioration than the leptin and curcumin.
Effect of letrozole on spermogram parameters and hormonal profile in infertile men: A clinical trial study
Authors: Peivandi Sepideh , Jafarpour Hamed , Abbaspour Mina , Ebadi Aghdas 
Published: November 2019

Abstract

Objective. There is no reliable treatment for men with idiopathic infertility, but the relationship between severe sperm production and the ratio of estrogen to testosterone levels has been shown. Aromatase is an enzyme that plays an important role in converting testosterone to estradiol and androstenedione to estrogen. Aromatase inhibitors can increase testosterone and androgen production without increasing the amount of estrogen in circulation. The aim of this study was to evaluate the effect of aromatase inhibitor letrozole on the male infertility.

Method. This pre- and post-quasi-experimental clinical trial was carried out on 41 men with an infertility diagnosis. The basic hormonal profile included FSH, LH, testosterone, and estradiol. The ratio of testosterone to serum estradiol was also calculated and recorded. The sperm analysis was performed before the treatment and the seminal parameters were evaluated and recorded. Patients were then treated with letrozole 2.5 mg daily for 4 months. At the end of 4th months, the hormonal profile was studied and seminal analysis performed and recorded.

Results. The levels of FSH, LH, testosterone, and estradiol, and the ratio of testosterone to estradiol increased significantly after letrozole treatment. The sperm concentration, sperm motility, and sperm forward motion significantly increased after letrozole treatment. Sperm morphology only lightly altered.

Conclusion. The ratio of testosterone to estradiol levels in infertile men treated with aromatase inhibitor improved and caused changes in sperm parameters. Letrozole may be used to improve sperm parameters in infertile men with low serum testosterone to estradiol ratio.
Hypoxic regulation of EDN1, EDNRA, EDNRB, and ECE1 gene expressions in ERN1 knockdown U87 glioma cells
Authors: Minchenko Dmytro O. , Tsymbal Daria O. , Riabovol Olena O. , Viletska Yuliia M. , Lahanovska Yuliia O. , Sliusar Myroslava Y. , Bezrodnyi Borys H. , Minchenko Oleksandr H. 
Published: November 2019

Abstract

Objective. The aim of the present investigation was to study the effect of hypoxia on the expression of genes encoding endothelin-1 (EDN1) and its cognate receptors (EDNRA and EDNRB) as well as endothelin converting enzyme 1 (ECE1) in U87 glioma cells in response to inhibition of endoplasmic reticulum stress signaling mediated by ERN1/IRE1 (endoplasmic reticulum to nucleus signaling 1) for evaluation of their possible significance in the control of glioma growth through ERN1 and hypoxia.

Methods. The expression level of EDN1, EDNRA, EDNRB, and ECE1 genes as well as micro-RNA miR-19, miR-96, and miR-206 was studied in control and ERN1 knockdown U87 glioma cells under hypoxia by quantitative polymerase chain reaction.

Results. It was shown that the expression level of EDN1, EDNRA, EDNRB, and ECE1 genes was up-regulated in ERN1 knockdown glioma cells in comparison with the control glioma cells, being more significant for endothelin-1. We also observed down-regulation of microRNA miR-206, miR-96, and miR-19a, which have specific binding sites in mRNA EDN1, EDNRA, and EDNRB, correspondingly, and can participate in posttranscriptional regulation of these mRNA expressions. Furthermore, inhibition of ERN1 endoribonuclease lead to up-regulation of EDNRA and ECE1 gene expressions and down-regulation of the expression level of EDN1 and EDNRB genes in glioma cells. Thus, the expression of EDNRA and ECE1 genes is regulated by ERN1 endoribonuclease, but EDN1 and EDNRB genes preferentially by ERN1 protein kinase. We have also shown that hypoxia enhanced the expression of EDN1, EDNRA, and ECE1 genes and that knockdown of ERN1 signaling enzyme function significantly modified the response of all studied gene expressions to hypoxia. Thus, effect of hypoxia on the expression level of EDN1 and ECE1 genes was significantly or completely reduced in ERN1 knockdown glioma cells since the expression of EDNRA gene was down-regulated under hypoxia. Moreover, hypoxia is induced the expression of EDNRB gene in ERN1 knockdown glioma cells.

Conclusions. Results of this investigation demonstrate that ERN1 knockdown significantly increased the expression of endothelin-1 and its receptors as well as ECE1 genes by different mechanisms and that all studied gene expressions were sensitive to hypoxia. It is possible that hypoxic regulation of the expression of these genes is a result of complex interaction of variable ERN1 related transcription and regulatory factors with HIF1A and possibly contributed to the control of glioma growth.