bluuebunny/crossref_metadata_2025_split huggingface dataset:
A G protein-coupled receptor-like module regulates Cellulose Synthase secretion from the endomembrane system in Arabidopsis
Authors: McFarlane Heather E. , Mutwil-Anderwald Daniela , Verbančič Jana , Picard Kelsey L. , Gookin Timothy E. , Froehlich Anja , Trindade Luisa M. , Alonso Jose M. , Assmann Sarah M. , Persson Staffan 
Published: October 2020

AbstractCellulose synthesis is essential for plant morphology, water transport and defense, and provides raw material for biomaterials and fuels. Cellulose is produced at the plasma membrane by Cellulose Synthase (CESA) protein complexes (CSCs). CSCs are assembled in the endomembrane system and then trafficked from the Golgi apparatus andtrans-Golgi Network (TGN) to the plasma membrane. Since CESA enzymes are only active in the plasma membrane, control of CSC secretion is a critical step in the regulation of cellulose synthesis. However, the regulatory framework for CSC secretion is not clarified. In this study, we identify members of a family of seven transmembrane domain-containing proteins (7TMs) as important for cellulose production during cell wall integrity stress. 7TM proteins are often associated with guanine nucleotide-binding protein (G) protein signalling and mutants in several of the canonical G protein complex components phenocopied the7tmmutant plants. Unexpectedly, the 7TM proteins localized to the Golgi apparatus/TGN where they interacted with the G protein complex. Here, the 7TMs and G proteins regulated CESA trafficking, but did not affect general protein secretion. Furthermore, during cell wall stress, 7TMs’ localization was biased towards small CESA-containing vesicles, specifically associated with CSC trafficking. Our results thus outline how a G protein-coupled module regulates CESA trafficking and reveal that defects in this process lead to exacerbated responses upon exposure to cell wall integrity stress.
Attentional bias to threat and gray mater volume morphology in high anxious individuals
Authors: Carlson Joshua M. , Fang Lin 
Published: October 2020

AbstractIn a sample of highly anxious individuals, the relationship between gray matter volume brain morphology and attentional bias to threat was assessed. Participants performed a dot-probe task of attentional bias to threat and gray matter volume was acquired from whole brain structural T1-weighted MRI scans. The results replicate previous findings in unselected samples that elevated attentional bias to threat is linked to greater gray matter volume in the anterior cingulate cortex, middle frontal gyrus, and striatum. In addition, we provide novel evidence that elevated attentional bias to threat is associated with greater gray matter volume in the right posterior parietal cortex, cerebellum, and other distributed regions. Lastly, exploratory analyses provide initial evidence that distinct sub-regions of the right posterior parietal cortex may contribute to attentional bias in a sex-specific manner. Our results illuminate how differences in gray matter volume morphology relate to attentional bias to threat in anxious individuals. This knowledge could inform neurocognitive models of anxiety-related attentional bias to threat and targets of neuroplasticity in anxiety interventions such as attention bias modification.
High Fat Diet Stimulates Beta-Oxidation, Alters Electrical Properties and Induces Adipogenicity of Atria in Obese Mice
Authors: Suffee Nadine , Baptista Elodie , Piquereau Jérôme , Ponnaiah Maharajah , Doisne Nicolas , Ichou Farid , Lhomme Marie , Pichard Camille , Galand Vincent , Mougenot Nathalie , Dilanian Gilles , Lucats Laurence , Balse Elise , Mericskay Mathias , Le Goff Wilfried , Hatem Stéphane 
Published: October 2020

SUMMARYMetabolic disorders such as obesity are risk factors of atrial fibrillation, not only by sharing comorbidities but likely through their direct impact on atria, notably its adipogenicity. Here, we submitted mice that lack cardiac adipose tissue to a high fat diet and first studied the atrial metabolomic and lipidomic phenotypes using liquid chromatography-mass spectrometry. We found an increased consumption of free fatty acid by the beta-oxidation and an accumulation of long-chain lipids in atria of obese mice. Free fatty acid was the main substrate of mitochondrial respiration studied in the saponin-permeabilized atrial muscle. Conducted action potential recorded in atrial trabeculae was short, and ATP-sensitive potassium current was increased in perforated patch-clamp atrial myocytes of obese mice. There was histological and phenotypical evidence for an accumulation of adipose tissue in obese mice atria. Thus, an obesogenic diet transforms the energy metabolism, causes fat accumulation and induces electrical remodeling of atria myocardium.HIGHLIGHTS- Untargeted metabolomic and lipidomic analysis revealed that a high fat diet induces profound transformation of atrial energy metabolism with beta-oxidation activation and long-chain lipid accumulation.- Mitochondria respiration studied in atrial myocardial trabecula preferentially used Palmitoyl-CoA as energy substrate in obese mice.- Atria of obese mice become vulnerable to atrial fibrillation and show short action potential due to the activation of K-ATP dependent potassium current.- Adipocytes and fat molecular markers were detected in atria of obese mice together with an inflammatory profile consistence with a myocardial accumulation of fat.
Single-cell metabarcoding reveals biotic interactions of the Arctic calcifierNeogloboquadrina pachydermawith the eukaryotic pelagic community
Authors: Greco Mattia , Morard Raphaël , Kucera Michal 
Published: October 2020

AbstractIsotopic and trace-element signals in the calcite shells of the planktonic foraminiferaNeogloboquadrina pachydermarepresent key proxies to reconstruct past climatic conditions in northern high latitudes. A correct interpretation of these chemical signals requires knowledge of the habitat and trophic interactions of the species. Direct observations on the biological interactions ofN. pachydermain polar environments are lacking and to date no consensus exists on the trophic behaviour of this species. Here we use single-cell metabarcoding to characterise the interactions of 39 specimens ofN. pachydermafrom two sites in the Baffin Bay with the local eukaryotic pelagic community. Our results show that the eukaryotic interactome of the foraminifera is dominated by diatoms, accounting for > 50% of the reads in 17 of the samples, but other groups such as Crustacea and Syndiniales are also present. The high abundance Syndiniales suggests that these parasites could infectN. pachydermaand may play an important role in its population dynamics. Moreover, the strong but taxonomically non-specific association with algae, existing irrespective of depth and occurring in specimens collected far below the photic zone indicates that opportunistically grazed diatom-fuelled marine aggregates likely represent the main interaction substrate ofN. pachyderma.
RhoA- and Ran-induced antagonistic forces underlie symmetry breaking and spindle rotation in mouse oocytes
Authors: Dehapiot Benoit , Clément Raphaël , Bourdais Anne , Huet Sébastien , Halet Guillaume 
Published: October 2020

AbstractMammalian oocyte meiotic divisions are highly asymmetric and produce a large haploid gamete and two small polar bodies. This relies on the ability of the cell to break symmetry and position its spindle close to the cortex before the anaphase occurs. In metaphase II arrested mouse oocytes, the spindle is actively maintained close and parallel to the cortex, until the fertilization triggers the sister chromatids segregation and the rotation of the spindle. The latter must indeed reorient perpendicular to the cortex to enable the cytokinesis ring closure at the base of the polar body. However, the mechanisms underlying symmetry breaking and spindle rotation have remained elusive. In this study, we show that the spindle rotation results from two antagonistic forces. First, an inward contraction of the cytokinesis furrow dependent on RhoA signaling and second, an outward attraction exerted on both lots of chromatids by a RanGTP dependent polarization of the actomyosin cortex. By combining live segmentation and tracking with numerical modelling, we demonstrate that this configuration becomes unstable as the ingression progresses. This leads to spontaneous symmetry breaking, which implies that neither the rotation direction nor the lot of chromatids that eventually gets discarded are biologically predetermined.
The E3 ligase TRIM1 ubiquitinates LRRK2 and controls its localization, degradation, and toxicity
Authors: Stormo Adrienne E. D. , Shavarebi Farbod , FitzGibbon Molly , Earley Elizabeth M. , Ahrendt Hannah , Lum Lotus S. , Verschueren Erik , Swaney Danielle L. , Skibinski Gaia , Ravisankar Abinaya , van Haren Jeffrey , Davis Emily J. , Johnson Jeffrey R. , Dollen John Von , Balen Carson , Porath Jacob , Crosio Claudia , Mirescu Christian , Iaccarino Ciro , Dauer William T. , Nichols R. Jeremy , Wittmann Torsten , Cox Timothy C. , Finkbeiner Steve , Krogan Nevan J. , Oakes Scott A. , Hiniker Annie 
Published: October 2020

AbstractMissense mutations in leucine-rich repeat kinase 2 (LRRK2) are the most common cause of familial Parkinson’s Disease (PD); however, pathways regulating LRRK2 subcellular localization, function, and turnover are not fully defined. We performed quantitative mass spectrometry-based interactome studies to identify 48 novel LRRK2 interactors, including the microtubule-associated E3 ubiquitin ligase TRIM1 (TripartiteMotif Family 1). TRIM1 recruits LRRK2 to the microtubule cytoskeleton for ubiquitination and proteasomal degradation by binding LRRK2911-920, a nine amino acid segment within a flexible interdomain region (LRRK2853-981), which we designate the “Regulatory Loop” (RL). Phosphorylation of LRRK2 Ser910/Ser935 within LRRK2 RL serves as a molecular switch controlling LRRK2’s association with cytoplasmic 14-3-3 versus microtubule-bound TRIM1. Association with TRIM1 modulates LRRK2’s interaction with Rab29 and prevents upregulation of LRRK2 kinase activity by Rab29 in an E3-ligase-dependent manner. Finally, TRIM1 rescues neurite outgrowth deficits caused by PD-driving mutant LRRK2 G2019S. Our data suggest that TRIM1 is a critical regulator of LRRK2, controlling its degradation, localization, binding partners, kinase activity, and cytotoxicity.
Claim tenability assessment in Indian real estate projects using ANN and decision tree models
Authors: Rai Himanshu , Jagannathan Murali , Delhi Venkata Santosh Kumar 
Published: October 2020

PurposeClaims have become an inseparable part of construction projects across the world. Construction claims often tend to result not only in time and cost overruns but in case of a dispute arising from the claim, it may result in erosion of the brand value and the working relationship between the parties. Thus, construction claim prediction is important but is complicated because of a large number of dependent factors and the complex inter-relations between them. With the aid of machine learning techniques, claim tenability assessment for real estate projects in India is attempted in this paper.Design/methodology/approachIn this research, artificial neural network (ANN) and decision tree models are used for assessment of claims in the Indian real estate sector using project and claims data from 275 real estate projects.FindingsThe developed ANN model assesses the claim tenability in a project with a high degree of accuracy. Both ANN and decision tree models identify that “inconsistency between drawings and specification” as the most influencing factor in claim tenability assessment.Research limitations/implicationsNotwithstanding the claim tenability assessment, the model, in its current form, cannot be used to predict the “extent of claim” in the real estate projects.Originality/valueClaim tenability assessment in real estate projects, especially in India, is scantily discussed in literature. This research, by adding to the body of knowledge, helps in both claim assessment and identification of factors that need to be controlled to reduce the claim tenability in real estate construction projects in India.
Measuring future resilience: a multilevel index
Authors: Visser Wayne 
Published: October 2020

Purpose
For the purpose of applied research, we are interested in deriving and measuring multi-level future resilience, from a human capital perspective. This paper aims to set the theoretical foundations for a future resilience index to be launched in 2020.

Design/methodology/approach
Based on a review of the literature on individual, organisational and socio-ecological resilience, the researcher has distilled 10 elements of future resilience. These were elaborated into a 20-question index, which has been piloted with an anonymous organisation.

Findings
The 10 elements of future resilience, which the index will seek to measure include: emergency preparedness; creative adaptability; technological empowerment; dynamic employability; diversity cultivation; participative governance; systemic responsiveness; resource efficiency; purposeful motivation; and well-being orientation. Illustrative findings from the pilot show that organisational support for resilience across all 10 categories is seen as weaker than individuals’ perception of their own level of resilience.

Practical implications
The areas of strong versus weak performance revealed by the index, either individually or in terms of organisational support, give organisations a clear set of priorities for follow-up.

Social implications
This paper has demonstrated that future resilience is a highly relevant and useful concept for society, organisations and individuals in these rapidly changing times.

Originality/value
Through the future resilience index, being developed by Antwerp Management School in collaboration with Randstad, this paper wants to encourage behaviours and capacities amongst employees that will increase resilience at the individual, organisational and socio-ecological levels. This is the first multi-level resilience measurement instrument aimed at human capital measurement.
How does review disconfirmation influence customer online review behavior? A mixed-method investigation
Authors: Li Hengyun , Meng Fang , Pan Bing 
Published: October 2020

Purpose
With the growing online review manipulation and fake reviews in the hospitality industry, it is not uncommon that a consumer encounters disconfirmation when comparing the existing online reviews with his/her own product or service evaluation. The purpose of this study is to investigate the influence of review disconfirmation on customer online review writing behavior.

Design/methodology/approach
This study used a mixed-method combining online secondary big data modeling and experimental design.

Findings
Review disconfirmation influences customers’ emotional responses embedded in the review; a customer who encounters review disconfirmation tends to exert more reviewing effort, manifested by writing longer reviews; negativity bias exists in disconfirmation effects, in that negative review disconfirmation shows more significant and stronger effects than positive review disconfirmation.

Practical implications
Findings from this study provide important managerial implications for business owners and marketers who attempt to influence online reviews. The study suggests that fictitious online review manipulation might be detrimental to the business.

Originality/value
This research contributes to two literature streams, including research on the social influence of online consumer reviews, and the relationship between disconfirmation and consumers’ post-consumption behavior, by extending the influence of disconfirmation from the offline context to the online context.
Transformative learning in the entrepreneurship education process: the role of pedagogical nudging and reflection
Authors: Neergaard Helle , Robinson Sarah , Jones Sally 
Published: October 2020

PurposeThis paper introduces “pedagogical nudging” as a method, which can transform student dispositions and their perceived “fit” with the field of entrepreneurship. The authors investigate what characterises the identity change process experienced by students when exposed to pedagogical nudging.Design/methodology/approachUsing ethnography, the authors apply an experiential-explorative approach to collecting data. The authors collected 1,015 individual reflection logs from 145 students of which the authors sampled 290 for this paper combined with interviews, observational and documentary data.FindingsPedagogical nudging techniques help (1) expose and challenge the student habitus by planting footprints in the mind; (2) straddle the divide between student and nascent entrepreneur by enabling them to recognise and experiment with an entrepreneurial habitus and (3) figuratively learn to climb the entrepreneurial tree by embracing an entrepreneurial habitus. In the first step, the authors use the interventions as cognitive means of influencing (pedagogical nudging). In the second, students participate in an iterative meaning-making process through reflection. In the third, they internalise the “new” entrepreneurial habitus—or discard it.Research limitations/implicationsThe authors extend existing knowledge about the effect of particular kinds of pedagogies in entrepreneurship teaching, and how these can support enterprising behaviour. The authors demonstrate how an exploration of the inner self, identity and beliefs develops the capacity for students to re-shape future outcomes and create value.Practical implicationsBy using nudging pedagogies, educators can support students to develop new ways of acknowledging and coping with transformative learning.Originality/valueThe research documents how it is possible to 'nudge' our students towards more entrepreneurial behaviours.
Online disinhibition: conceptualization, measurement, and implications for online deviant behavior
Authors: Cheung Christy M.K. , Wong Randy Yee Man , Chan Tommy K.H. 
Published: October 2020

PurposeOnline disinhibition is one of the key factors leading to the occurrence of cyberaggression, cyberbullying and various forms of deviant behaviors in the online environment. To understand the composition of online disinhibition, this study aims to conceptualize online disinhibition and develop a measurement instrument for online disinhibition.Design/methodology/approachWe followed a rigorous procedure to develop and validate the multidimensional instrument of online disinhibition in three phases: item generation, measurement development and instrument testing.FindingsWe developed a 23-item online disinhibition scale and identified six key dimensions: dissociative anonymity, invisibility, asynchronicity, solipsistic introjections, dissociative imagination and minimization of authority.Practical implicationsThe online disinhibition instrument is an accessible and easily administered measure that can be used as a checklist for systems designers and administrators to evaluate the level of online disinhibition among users. It offers systems design information on how to prevent and combat online deviant behaviors on platforms.Originality/valueThis work provides a rich conceptualization of an online disinhibition instrument that can serve as a springboard for future work to understand online deviant behaviors. The newly developed measurement instrument of online disinhibition also adds to the repository of rigorous research scales in this area.
T4‐like myovirus community shaped by dispersal and deterministic processes in the South China Sea
Authors: Li Huifang , Liu Lu , Wang Yu , Cai Lanlan , He Maoqiu , Wang Long , Hu Chen , Jiao Nianzhi , Zhang Rui 
Published: October 2020

SummaryAs the most abundant and genetically diverse biological entities, viruses significantly influence ecological, biogeographical and evolutionary processes in the ocean. However, the biogeography of marine viruses and the drivers shaping viral community are unclear. Here, the biogeographic patterns of T4‐like viruses and the relative impacts of deterministic (environmental selection) and dispersal (spatial distance) processes were investigated in the northern South China Sea. The dominant viral operational taxonomic units were affiliated with previously defined Marine, Estuary, Lake and Paddy Groups. A clear viral biogeographic pattern was observed along the environmental gradient from the estuary to open sea. Marine Groups I and IV had a wide geographical distribution, whereas Marine Groups II, III and V were abundant in lower‐salinity continental or eutrophic environments. A significant distance‐decay pattern was noted for the T4‐like viral community, especially for those infecting cyanobacteria. Both deterministic and dispersal processes influenced viral community assembly, although environmental selection (e.g. temperature, salinity, bacterial abundance and community, etc.) had a greater impact than spatial distance. Network analysis confirmed the strong association between viral and bacterial community composition, and suggested a diverse ecological relationship (e.g. lysis, co‐infection or mutualistic) between and within viruses and their potential bacterial hosts.
LncRNA SNHG20 promoted proliferation, invasion and inhibited cell apoptosis of lung adenocarcinoma via sponging miR‐342 and upregulating DDX49
Authors: Wang Xiuli , Gu Guomin , Zhu Hongge , Lu Suqiong , Abuduwaili Kahaerjiang , Liu Chunling 
Published: October 2020

BackgroundThere is increasing evidence that long non‐coding RNA (lncRNA) small nucleolar RNA host gene 20 (SNHG20) plays an important role in cancer. However, the function of SNHG20 in lung adenocarcinoma is unclear. The aim of our study was to investigate the roles of SNHG20 in lung adenocarcinoma.MethodsReal‐time quantitative polymerasechain reaction (RT‐qPCR) was used to calculate the expression of SNHG20, miR‐342 and DEAD‐box helicase 49 (DDX49). Dual luciferase reporter gene assay was applied to verify whether miR‐342 binding to SNHG20 and DDX49. The expression correlation between miR‐342 and SNHG20 or DDX49 was assessed using Pearson's correlation analysis.ResultsSNHG20 and DDX49 were overexpressed, while miR‐342 was lowly expressed in lung adenocarcinoma tissues and cell lines. Knockdown of SNHG20 suppressed cell proliferation, invasion and enhanced cell apoptosis. SNHG20 was found to directly bind to miR‐342 and regulate the expression of miR‐342. MiR‐342 directly targeted DDX49 and the expression of miR‐342 had negative connection with DDX49 in lung adenocarcinoma tissues. Knockdown of DDX49 inhibited the progression of lung adenocarcinoma. DDX49 partially restored the functions of SNHG20 in A549 cells.ConclusionsSNHG20 regulated lung adenocarcinoma cell proliferation, invasion and promoted cell apoptosis via miR‐342/DDX49 axis. Our findings demonstrate that SNHG20/miR‐342/DDX49 axis plays an important role in lung adenocarcinoma, providing a novel insight into the treatment of lung adenocarcinoma.