bluuebunny/crossref_metadata_2025_split huggingface dataset:
Urinary Exosome Based Androgen Receptor Variant 7 Detection in Metastatic Castration Resistant Prostate Cancer Patient
Authors: Wang Chao , Liu Xiang , Li Hongyan , Zhao Libo , Kong Guanyi , Chen Jing , Li Zhi , Qi Jianfei , Tian Ye , Zhang Fengbo 
Published: July 2020

Abstract
BACKGROUND Androgen receptor variant 7 (AR-V7) detection provides important reference to the clinical management of abiraterone in metastatic castration-resistant PCa (mCRPC). We performed a noninvasive urine-derived exosomal AR-V7 analysis in mCRPC patients. METHODS A total of 34 mCRPC patients were categorized into 16 patients responded to abiraterone (ABI-Sta group) and 18 were resistant to abiraterone (ABI-Res group). First morning urine collected from patients as well as healthy control were used for analysis. Exosomal RNA was isolated for AR-V7 detection, and the correlation between AR-V7 expression and the therapeutic effect of abiraterone was analyzed.RESULTS Urine is ideal biological sample for exosome separation and androgen receptor full-length (AR-FL) analysis. Positive urine-derived exosomal AR-V7 were detected in 32.4% (11 of 34) mCRPC urine samples. Positive AR-V7 is more common in ABI-Res patients (50.0% in ABI-Res group vs. 12.5% in ABI-Sta patients respectively, p=0.009) and associate with higher PSA progression rate and poorer overall survival (p=0.0031, 0.0012). CONCLUSIONS Urine-derived exosome is an alternative option for liquid biopsy-based AR-V7 detection in prostate cancer. Urinary exosomal AR-V7 is a dynamic marker that may predict the response to abiraterone in mCRPC patients.
Systematic Review and Bibliometric Analysis of African Anesthesia and Critical Care Medicine Research Part I: Hierarchy of Evidence and Scholarly Productivity
Authors: Kanmounye Ulrick Sidney , Tochie Joel Noutakdie , Mbonda Aimé Noula , Wafo Cynthia Solam , Daya Leonid , Atem Thompson Hope , Nyalundja Arsène Daniel , Eyaman Daniel Cheryl 
Published: July 2020

Abstract
BackgroundResearch is an essential component of Anesthesia, and the contributions of researchers and institutions can be appreciated from the analysis of scholarly outputs. Such analyses help identify major contributors and trends in publication. Little is known about the state of Anesthesia and Critical Care Medicine (ACCM) research in Africa. In this study, the authors analyzed the study designs and contributions to ACCM research in Africa.MethodsThe authors searched PubMed, Embase, Web of Science, and CINAHL from inception to May 4, 2020, for articles on or about ACCM in Africa. The titles and abstracts of the articles were screened first on Rayyan. Then, the full-text of eligible articles were screened. Data were extracted, and the number of articles per physician anesthesia providers and provider density were calculated. Kruskal Wallis test and Spearman's correlation were used, and a P-value <0.05 was considered to be statistically significant.ResultsOf the 4690 articles, only 886 (18.9%) were included in the analysis. The articles were published between 1946 and 2020 in 278 target journals. 55 (6.2%) articles were published in the South African Journal of Surgery, 51 (5.8%) in Anesthesia and Analgesia, and 46 (5.2%) in Anaesthesia. 291 (32.8%) studies were cross-sectional. 195 (22.0%) first authors were from Nigeria, 118 (13.3%) from South Africa, and 88 (9.9%) from the USA. Malawi (1.67), Togo (1.06), and Sierra Leone (1.00) had the highest number of articles per provider. Whereas Ethiopia (580.00), Nigeria (336.21), and Malawi (333.33) had the highest number of articles per provider density. ConclusionThe results reported in this study highlight contributions of Africans and the hierarchy of evidence in African anesthesiology research and critical care medicine. These results may aid in setting the research agenda.
LncRNA HOTAIR regulates the expression of E-cadherin to affect nasopharyngeal carcinoma progression by recruiting histone methylase EZH2 to mediate H3K27 trimethylation
Authors: Yang Feng-lian , Wei Yu-xia , Liao Bi-yun , Wei Gui-jiang , Qin Hai-mei , Pang Xiao-xia , Wang Jun-li 
Published: July 2020

Abstract
Background/Aim:
In recent years, there has been increasing evidence for the function of long non-coding RNA (lncRNA) in nasopharyngeal carcinoma (NPC).. We aim to delve into the position of lncRNA HOTAIR, together with EZH2, E-cadherin and H3K27me3 in NPC and explore the related mechanisms.
Methods
RT-qPCR and western blot analysis were carried out for detecting lncRNA HOTAIR, EZH2, E-cadherin and H3K27me3 expression in NPC tissues and cells. Moreover, the correlations between lncRNA HOTAIR and EZH2 expression and the clinicopathological characteristics and prognosis of patients with NPC were observed. NPC cell biological functions were examined through gain-of and loss-of function assays. RIP and ChIP assays were applied to detect whether lncRNA HOTAIR in NPC cells could regulate E-cadherin by recruiting EZH2 to mediate trimethylation of H3K27.
Results
LncRNA HOTAIR, EZH2, and H3K27me3 were richly expressed in NPC tissues and cells, and E-cadherin was lowly expressed. The prognosis of patients with overexpression of lncRNA HOTAIR and EZH2 was worse than that of patients with theirs low expression. Down-regulation of either HOTAIR or EZH2 inhibited cell proliferation, promoted apoptosis, suppressed migration and invasion and inhibited tumor growth. HOTAIR recruited histone methylase EZH2 to mediate trimethylation of H3K27 and regulated E-cadherin expression.
Conclusion
Our study suggests that lncRNA HOTAIR inhibits the expression of E-cadherin by stimulating the trimethylation of H3K27 by histone methylase EZH2 to promote cell migration, proliferation, and inhibit apoptosis of NPC cells.
Knowledge, Attitudes, Practices and Risk factors about Malaria in High Endemic Rural Eastern Tanzania
Authors: Mnyone Ladslaus Laurent , Mwamundela Baraka 
Published: July 2020

Abstract
Background: Understanding area-specific knowledge, attitudes, practices and demographic risk factors towards malaria is becoming critical for designing appropriate awareness and control strategies. Methods: A cohort of respondents from 243 randomly selected households across 3 villages in eastern Tanzania was assessed using a standard questionnaire and direct observation.Results: Fifty-three percent of respondents cited Anopheles mosquitoes that were responsible for transmitting malaria, and 85% of those explicitly cited female Anopheles mosquitoes. The rest (47%) knew that malaria is transmitted by a mosquito but could not name it. Commonly cited mosquito breeding sites were water bodies (75%) and rubbish (24%). Majority (61%) knew that the under-fives were most vulnerable to malaria. ITNs were mostly (64%) cited as a means of malaria control. Commonly cited malaria mosquito entry routes were windows (46%), eave opening (10%) and opening on the walls (4%). Majority (70%) confirmed malaria at the health facility. The rest (30%) relied on symptoms. Majority (96%) preferred modern antimalarials. Seventy-nine percent of the respondents owned at least one ITN. Cooking was done predominantly (77%) outdoors, finished cooking and went to bed after 20:00h. Most (58%) respondents still attended night-time outdoor communal gatherings without malaria protection. Conclusions: The current study indicate that majority of participants were aware that Anopheles mosquitoes were responsible for transmitting malaria, they bred in water, under-five children were most vulnerable to malaria, and ITNs constituted the most deployed control measure. Most respondents preferred confirming malaria at the health facility and treatment with modern antimalarials. Yet, the rate of self-diagnosis and self-medication, as well as inappropriate use of bednets requires great improvement. Demographic risk factors for early- and/or outdoor-transmission included night-time outdoor cooking and attending communal gatherings without malaria protection. The findings warrant a subsequent study to show a causal link between night-time outdoor activities, vector biting patterns and malaria prevalence.
An optimized segmentation and quantification approach in microvascular imaging for OCTA-based fibrovascular regression monitoring
Authors: Wu Sheng , Wu Shaowei , Feng Hui , Hu Zizhong , Xie Yejing , Su Yun , Feng Ting , Li Li 
Published: July 2020

Abstract
Background:

Quantification of neovascularization changes in fibrovascular membrane (FVM) acquired from optical coherence tomography angiography (OCTA) imaging is extremely important for diagnosis and treatment monitoring of proliferative diabetic retinopathy (PDR). However, few vessel extraction methods have been reported for quantifying the neovascular changes on fibrovascular membrane (FVM) in proliferative diabetic retinopathy PDR based on OCTA imaging.
Results:

The proposed method has achieved better performance compared with existing algorithms on accuracy and can be used for PRD treatment monitoring. In the study of PDR treatment monitoring, the data show that from the beginning (0 day) to 5th day of treatment, the total length of neovascularization on FVM in this area has been significantly shortened by an 77.8% reduction, indicating significant effects from the treatment applied. Besides, the average width of the neovascularization on FVM at the 7th day after treatment has been increased by 158%, which indicates that most of the narrow neovascularization has been reduced.
Conclusion:

The result and analysis have confirmed that the proposed optimization process with improved VCA method is both effective and feasible to segment and quantify the neovascularization on FVM with less noise and artifacts, thus can be readily applied to monitor the fibrovascular regression within the treatment period.
The presence of aura is not related to changes in the cervical performance and mobility of patients with migraine
Authors: Carvalho Gabriela F , Lodovichi Samuel S , Pinheiro Carina F , Benatto Mariana T , Florencio Lidiane L , Bragatto Marcela M , Dach Fabiola , Bevilaqua-Grossi Debora 
Published: July 2020

Abstract
Background: Migraine is related to musculoskeletal impairment of the neck and the chronicity of migraine is related to greater disability levels. However, it is not known if other forms of migraine, such as migraine with aura, is related to a different pattern of neck impairment.Objective: To assess the musculoskeletal and clinical aspects of the neck in patients with migraine with and without aura.Methods: Up to 125 consecutive patients diagnosed with migraine according to the ICHD-III were recruited from a tertiary headache clinic. Patients were classified by the presence of aura into two groups: migraine with aura (MA, n=37) and migraine without aura (MoA, n=88). The self-report of neck pain was assessed and patients completed the Neck Disability Index (NDI) questionnaire. Furthermore, patients performed the Flexion Rotation Test (FRT) and the Cranio-Cervical Flexion Test (CCFT). Results of all outcomes were contrasted between groups through Mann-Whitney and Qui-square tests.Results: There was no association between the presence of aura and self-report of neck pain (x²: 1.32, p=0.25). No differences were verified between groups regarding presence of neck disability (MA: 10.73, SD: 6.22; MoA: 9.63, SD:8.13, p=0.25) or disability severity (x²=6.17, p=0.10). Groups did not differ regarding the FRT (MA: 35.07°, SD: 7.90 and MoA: 34.60°, SD: 8.70, t=-0.22, p=0.83) and there was no association between positive FRT and presence of aura (x²=3.35, p=0.07). The CCFT did not demonstrate differences among the two groups either (x²=1.65, p=0.80).Conclusion: There is no association between migraine aura and presence or disability of neck pain, reduced superior cervical mobility or reduced neck performance. It is expected to find similar neck profile during the clinical assessment of patients with migraine with and without aura.
Amyl Nitrite Induced Hemolytic Anemia: Acute Therapy and Prevention
Authors: Elshikh Amira , Kango Ghazal , Baalbaki Marwa , Lankowsky Jeffrey , Bawa Amandeep 
Published: July 2020

Abstract
Background:
Inhaled nitrites have been a substance of recreational use for centuries, primarily among men who have sex with men (MSM). However, there is a lack of awareness of the use of inhaled nitrites in this population and the possible complications and health disparities it carries. This case report highlights the use of inhaled nitrites in an MSM male with an emphasis on the complications induced by this product, including severe hemolytic anemia and methemoglobinemia. Followed by a review of both acute therapy and preventative therapy by increasing provider awareness.
Case presentation:
A 62-year-old man with a past medical history of glucose-6 phosphate dehydrogenase (G6PD) deficiency presented to the emergency department with atypical chest pain. He was tachypneic and hypotensive with laboratory evidence of end organ damage. He had an acute drop in hemoglobin with elevated bilirubin concerning for acute hemolytic anemia. His arterial blood gas was significant for elevated methemoglobin. The patient reported recent use of “poppers” (inhaled nitrite) which is associated with formation of methemoglobin and hemolytic anemia. Given the severity of his symptoms and elevated methemoglobin, he met criteria for methylene blue administration. However, his G6PD deficiency is a contraindication to methylene blue and the patient was treated conservatively with a positive outcome.
Conclusion:
The following report presents a unique scenario in which a G6PD deficient patient presented with hemolytic anemia and methemoglobinemia after recreational use of inhaled nitrites. The case was complicated with the management challenge of methemoglobinemia in a patient with G6PD deficiency. Prior literature has shown that the use of nitrites is a predictor for high-risk sexual behavior and is associated with positive human immunodeficiency virus (HIV) status. Increasing provider awareness for appropriate counseling is of importance.
Predicting Prostate Cancer Upgrading of Biopsy Gleason Grade Group at Radical Prostatectomy Using Machine Learning-Assisted Decision-support Models
Authors: Liu Hailang , Tang Kun , Peng Ejun , Wang Liang , Xia Ding , Chen Zhiqiang 
Published: July 2020

Abstract
Objective: To develop a machine learning (ML)-assisted model capable of accurately predicting the probability of biopsy Gleason grade group upgrading before making treatment decisions by integrating multiple clinical characteristics.Materials and Methods: We retrospectively collected data from PCa (prostate cancer) patients who underwent systematic biopsy and radical prostatectomy from January 2015 to December 2019 at Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology. The study cohort was divided into training and testing datasets in a 70:30 ratio for further analysis. Four ML-assisted models were developed from 16 clinical features using logistic regression (LR), logistic regression optimized by Lasso regularization (Lasso-LR), random forest (RF) and support vector machine (SVM). The area under the curve (AUC) was applied to determine the model with the highest discrimination. Calibration plots were used to investigate the extent of over- or underestimation of predicted probabilities relative to the observed probabilities in models. Results: In total, 530 PCa patients were included, with 371 patients in the training dataset and 159 patients in the testing dataset. The Lasso-LR model showed good discrimination with an AUC, accuracy, sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of 0.776, 0.712, 0.679, 0.745, 0.730 and 0.695, respectively, followed by SVM (AUC 0.740, 95%CI: 0.690–0.790), LR (AUC 0.725, 95%CI: 0.674–0.776) and RF (AUC 0.666, 95%CI: 0.618–0.714). Validation of the model showed that the Lasso-LR model had the best discriminative power (AUC 0.735, 95%CI: 0.656–0.813), followed by SVM (AUC 0.723, 95%CI: 0.644–0.802), LR (AUC 0.697, 95%CI: 0.615–0.778) and RF (AUC 0.607, 95%CI: 0.531–0.684) in the testing dataset. Both the Lasso-LR and SVM models were well-calibrated. Conclusions: The Lasso-LR model had good discrimination in the prediction of patients at high risk of harboring incorrect Gleason grade group assignment, and the use of this model may be greatly beneficial to urologists in treatment planning, patient selection, and the decision-making process for PCa patients.
Baduanjin Exercise May Improve the Anxiety and Insomnia in COVID-2019 Patients: A Case-Control Study
Authors: Chen Ming-Gui , Qiu Yinlong , Chen Haizhen , Chen Erhui , Zeng Rui-Xiang , Wu Qiaomei , Zhang Xiaoxuan , Zhang Min-Zhou , Lin MeiZhen 
Published: July 2020

Abstract
Background: Since December 2019, an epidemic caused by novel coronavirus(2019-nCoV) infection has occurred unexpectedly in China. Because of the sudden nature of the outbreak and the infectious power of the virus, it will inevitably cause people anxiety and other stress reactions. Previous studies showed that Baduanjin exercise was effective for people in anxiety and insomnia. The purpose of this study was to evaluate the potential benefits of Baduanjin exercise on the anxiety and insomnia in COVID-2019 patients.Methods: This is a Case-Control Study. The COVID-2019 patients including 39 Baduanjin exercises or willing to do Baduanjin exercises and 39 age-and gender-matched nonexercising controls. The anxiety and insomnia in COVID-2019 patients were measured by using the GAD-7 and SMH Sleep Questionnaire at baseline and discharge.Results: In the study, the Paired T-test showed that two groups had improved the GAD-7 scores and SMH Sleep Questionnaire compared with baseline at discharge. Baseline results showed there were no significant differences in the GAD-7 scores and SMH Sleep Questionnaire between the two groups. However, the significant differences found in the Baduanjin group included a 43.9% lower (p<0.001) in the GAD-7 score and an approximately 75.9% higher (p=0.003) in SMH Sleep Questionnaire score compare with the control group at discharge.Conclusion: The Baduanjin exercise may improve the anxiety and insomnia in COVID-2019 patients. It can also be used as a form of rehabilitation exercise for discharged patients or patients isolated at home.Trial Registration: ChiCTR2000030528.
Refraining from Seeking Mental Healthcare and Poorer Mental Well-Being: A Longitudinal Population-Based study on Men in Sweden
Authors: Olsson Sara , Burström Bo , Hensing Gunnel , Löve Jesper 
Published: July 2020

Abstract
Background: Depression and anxiety disorder contribute to a significant part of the disease burden among men, yet many men refrain from seeking care or receive insufficient care when they do seek it. This is plausibly detrimental to men’s mental well-being, but there is a lack of population-based research to confirm this. This study investigated 1) if men who had refrained from seeking mental healthcare had poorer mental well-being than those who sought care, 2) if those who sought care but perceived it as insufficient had poorer mental well-being than those who perceived care as sufficient, and 3) if these differences persisted over time. Methods: This longitudinal study used questionnaire data from a population-based sample of 1240 men, aged 19-64 years, in Sweden. Having refrained from seeking mental healthcare, or perceiving the care as insufficient, at any time in life, was assessed in a questionnaire, 2008. Current mental well-being was assessed in both 2008 and 2009 using mean scores on the WHO (Ten) Well-being Index, with a lower score indicating poorer mental well-being. Group differences were calculated using t-tests and multivariable linear regression analysis.Results: Of the men who had perceived a need for mental healthcare, 37% refrained from seeking such care. They had lower mental well-being scores in 2008, compared to those who sought care. Of those seeking care, 29 % perceived it as insufficient. They had lower mental well-being scores in 2008, compared to those who perceived the care as sufficient, but this was not statistically significant when controlling for potential confounders in the regression analysis. There were no differences in mental well-being scores based on care-seeking or perceived care-sufficiency in 2009. Conclusions: This population-based study provides some empirical support for the hypothesis that refraining from seeking mental healthcare, or perceiving the care as insufficient, is detrimental to men’s mental well-being. However, the lack of persistent differences contradicts this hypothesis. The results highlight the need for more research, using larger population-based samples of men, and longer follow-up periods. This should be combined with efforts to increase men’s mental healthcare-seeking and to provide mental healthcare that is perceived as sufficient.
Accuracy and precision of zero-echo-time, single- and multi-atlas attenuation correction for dynamic [11C]PE2I PET-MR brain imaging
Authors: Sousa Joao M. , Appel Lieuwe , Merida Inés , Heckemann Rolf A. , Costes Nicolas , Engström Mathias , Papadimitriou Stergios , Nyholm Dag , Ahlström Håkan , Hammers Alexander , Lubberink Mark 
Published: July 2020

Abstract
Background: A valid photon attenuation correction (AC) method is instrumental for obtaining quantitatively correct PET images. Integrated PET/MR systems provide no direct information on attenuation, and novel methods for MR-based AC (MRAC) are still under investigation. Evaluations of various AC methods have mainly focused on static brain PET acquisitions. In this study we determined the validity of three MRAC methods in a dynamic PET/MR study of the brain.Methods: Nine participants underwent dynamic brain PET/MR scanning using the dopamine transporter radioligand [11C]PE2I. Three MRAC methods were evaluated: single-atlas (Atlas), multi-atlas (MaxProb) and zero-echo-time (ZTE). The 68Ge-transmission data from a previous stand-alone PET scan was used as reference method. Parametric relative delivery (R1) images and binding potential (BPND) maps were generated using cerebellar grey matter as reference region. Evaluation was based on bias in MRAC maps, accuracy and precision of [11C]PE2I BPND and R1 estimates, and [11C]PE2I time-activity curves. BPND was examined for striatal regions and R1 in clusters of regions across the brain.Results: For BPND, ZTE-MRAC showed the highest accuracy (bias < 2%). Atlas-MRAC exhibited a significant bias in caudate nucleus (-12%) while MaxProb-MRAC revealed a substantial, non-significant bias in putamen (9%). R1 estimates had a marginal bias for all MRAC methods (-1.0-3.2%). MaxProb-MRAC showed the largest intersubject variability for both R1 and BPND. Standardized uptake values (SUV) of striatal regions displayed the strongest average bias for ZTE-MRAC (~ 10%), although constant over time and with the smallest inter-subject variability. Atlas-MRAC had highest variation in bias over time (+ 10 to -10%), followed by MaxProb-MRAC (+ 5 to -5%), but MaxProb showed the lowest mean bias. For cerebellum, MaxProb-MRAC showed highest variability while bias was constant over time for Atlas- and ZTE-MRAC.Conclusions: Both Maxprob and ZTE-MRAC performed better than Atlas-MRAC when using a 68Ge transmission scan as reference method. Overall, ZTE-MRAC showed the highest precision and accuracy in outcome parameters of dynamic [11C]PE2I PET analysis with use of kinetic modelling.