bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Potency of Giving Turmeric (Curcuma domestica Val.) Flour to The Quality of Quail (Coturnix coturnix japonica) Eggs
Authors: Putri Astrid Annisya Adhy , Widodo Agus , Damayanti Ratna , Suprayogi Tri Wahyu 
Published: August 2020

Background: Supplementation of good feed on quail can improve its egg quality. One of the excellent supplement comes from herbs. Turmeric is one of the herbs which good for livestock because of its content, curcuminoid, and essential oil. Curcuminoid can increase quail egg yolk color, increase the appetite so that it can increase the weight of poultry. Purpose: To know the potential of giving turmeric flour to the quality of quail’s egg. Methods: The methods used 40 quails, divide into two groups. Groups P0: quail fed without turmeric flour, and group P1: quails fed with 1,07% turmeric fluor. Variables observed were egg weight, haugh unit, egg yolk index, white egg index, and yolk color. Result: The Egg weight, haugh unit, yolk index, and yolk color of P1 was higher than quails fed without turmeric. However, there was no difference between the egg whites index of both groups. Conclusion: The addition of turmeric on feed has the potential to increase the egg weight, haugh unit, yolk index, and yolk color. However, turmeric was not directly affected the formation of egg whites.
LANGUAGE STYLE AND PRESERVING LOCAL IDENTITY IN WAYANG BANYUMAS THROUGH VOCATIONAL HIGH SCHOOL STUDENTS’ PERFORMANCE
Authors: Adiarti Dian 
Published: August 2020

This research is aimed at identifying language style used in Wayang Banyumas and describing the local wisdom reflected in the students’ performance. Local wisdom or also known as local genius may be defined as the sum of the cultural characteristics which the vast majority of a people have in common as a result of their experiences in early life. This is a descriptive qualitative research since it comes up with the identification of language style used in Wayang Banyumas. The primary data were collected from Wayang Banyumas performed by the students of State Vocational High School (SMKN) 3 Banyumas; there were five performances entitled Bilawa Rangsang, Jarasanda Lena, Abiyasa Sayembara, Gendreh Kencana, and Wisangeni Krama respectively. To complete the data, the secondary data were gained from documentation and interview with some key informants who are expert of Wayang Banyumas for enriching the data description. The results reveal several language styles used in Wayang Banyumas dominated by the finding of figurative language and phonological features. Figurative languages found are: metaphor, simile, and hyperbole while the phonological features collected are assonance (purwakanti swara) and alliteration (purwakanti sastra) which belong to repetition of vowel or consonant sounds in a sequence of nearby words. Moreover, socio-cultural values and moral messages are also reflected in wayang performance. It taught the ideal figure of leader, the excellence of deliberation or musyawarah, peaceful act, and honesty.
TINDAK TUTUR ILOKUSI PENGAJAR PADA PROSES PEMBELAJARAN BAHASA MANDARIN
Authors: Herliana Monika , Suryadi M. 
Published: August 2020

Speech acts are divided into three types, namely locus speech acts, illocutionary speech acts, and perlocution speech acts. Illocutionary speech acts consist of assertive, declarative, commissive, expressive, and directive. Illocutionary can be defined as speech acts that often occur in daily interactions, one of these interactions covers the interaction between teachers and learners in educational activities in the classroom. In this activity, the teacher gives command to the students to follow the instructions. This study aims to determine the illocutionary speech acts contained in the interaction between teacher and students in using Mandarin in the classroom of D3 program of Mandarin Language, General Soedirman University, Purwokerto. This research belongs to a descriptive qualitative research. The data of this research are served in the form of speech between teacher and students during the learning of Mandarin Intermediate Conversation courses of D3 Mandarin Language Study Program UNSOED Purwokerto. The data analysis used theory of illocutionary speech acts by Austin and Searle. The data are presented in an informal form in which the data presentation and writing rules used words not numbers. The results showed that the interaction between teachers and learners in the D3 Mandarin Study Program of UNSOED Purwokerto cover assertive, declarative, commissive, expressive, and directive speech acts.
Effect of Variant Manure on Generation Time in Daphnia sp.
Authors: Hamdani Herman , Sahidin Asep , Hidayat Taufiq 
Published: August 2020

The research about generation times of Daphnia sp. was carried out on Mei – June 2017 in Biology Laboratory, FPIK UNPAD. The objective of this research was to analyse the generation time of Daphnia sp. in different manure. The research used experimental method Randomized Completely Block Design (RCBD) with four treatment and three replication. The variant of manure as treatment were chicken manure (A), quail manure (B), cow manure (C), and goat manure (D). The weight for each manure media as many as 2,4 g/l in 1 litre of glass media. The best performance of life table distribution showed from the cow manure, but quail manure treatment give the best of all biological factor, it mean that Daphnias were cultured with quail manure have good performance in biological parameter.
Polyculture Engineering of White Shrimp Vannamei and Seaweed on Different Planting Distance on The Growth, Survival in Abration Pond
Authors: Samidjan Istiyanto , Rachmawati Diana 
Published: August 2020

The objective of the study was to assess the role of polyculture engineering of vanamei shrimp, and seaweed in an effort to increase growth and survival. The experimental method was randomized complete design with 4 treatments and 3 replications were T1 (10 PL / m2 white shrimp vannamei and seaweed distance 15 cm), T2 (10 PL / m2 of white shrimp vannamei and seaweed distance 30 cm), T3 (10 PL / m2 white shrimp vanamei and seaweed distance 45 cm, T4 (10 PL / m2 white shrimp vanamei and seaweed 60 cm). Seaweed used Gracillaria sp type 150 g / bundle with treatment used a long line system placed around the nets plot of 1 m2 in pond culture 300 m2. Data includes the growth of absolute weight, survival, FCR were analyzed variety and test Tukey. observation of the water quality of maintenance media include nitrite, ammonia, temperature, pH, salinity using descriptive. There is a very real effect (P <0.01) on treatment with different plant spacing on seaweed and vanamei shrimp on growth and survival of white shrimp vanamei and seaweed. The highest absolute weight growth in vanamei shrimp was T4 treatment (vanamei shrimp 29.25 ± 0.07 g), and seaweed (2919.03 ± 8.70bg) of white shrimp vanamei survival rate (98.25% ± 2.25%) and FCR (food Conversion ratio) 1.15 ± 0.09. Water quality is still feasible optimum.
Composition of Microplastics Of Coastal Sediments In Kupang and Rote Isalnd, East Nusa Tenggara
Authors: Anggara Tombus Lucas , Yuliadi Lintang P. S. , Ihsan Yudi Nurul , Ismail M. Rudyansyah 
Published: August 2020

This research was conducted at the Kupang dan Rote Islands, East Nusa Tenggara in June until November 2018. This research using Wet Peroxide Oxidation methods and aims to obtain data on microplastics distributin that accumulates in coastal sediments in Kupang and Rote Islands. A result of this research shows that the lowest coast is Ndana Beach at 5 particles/400gr and Pasir Panjang has the highest microplastics abundancre of 23 particles/400gr. A microplastics obtained are fragments, fibers and films but for films that are obtained in 2 stations is Oenggae Beach and Pasir Panjang Beach. A microplastics colors obtained are black, blue, yellow, red, green and white and for sizes small microplastics dominant in all stations. Keywords: Kupang and Rote Island, Microplastic, Coastal Sediments, Wet Peroxide Oxdation
Survival Rate and Growth of Economical Fishes in Tailing Ponds of Bauxite Post-mining in Senggarang, Tanjungpinang City
Authors: Ashari Irvan Hasan , Apriadi Tri , Melani Winny Retna 
Published: August 2020

The tailing pond of bauxite post-mining in Senggarang, Tanjungpinang City, have been potency of natural feed sources from phytoplankton and zooplankton. Moreover, the water quality of the tailing pond of bauxite post-mining has begun to support fish life. The objective of this study was to determine the survival rate and growth of economical fish in tailings ponds of bauxite post-mining in Senggarang, Tanjungpinang. The research was conducted by field experiment using a completely randomized design. There were different fish varieties as treatment: tilapia (Oreochromis niloticus), gouramy (Osphronemus gouramy), and tambaqui (Colossoma macropomum). Every treatment was carried out 5 replications, with 6 fish in every cage replication. Monitoring the water quality and the fish survival on the 0th, 10th, 20th and 30th days. The survival of economical fishes in tailing ponds of bauxite post-mining from the highest to lowest were tambaqui (97%), tilapia (83%), and gouramy (40%), respectively. The growth of fishes length from the highest to the lowest were gouramy (0,223 cm), tambaqui (0,037 cm), and tilapia (0,018 cm). Meanwhile, all variety of fish tested had a decreased in weigh: gouramy -0,2310 grams, tilapia -0,4281 grams, and tambaqui -1,3498 grams. The management of tailing ponds of bauxite post-mining in Senggarang for fisheries activities can be carried out by tambaqui (C. macropomum) culture cage with several conditions such as measurement of carrying capacity and capacity, management of water quality, feed, and technical aspects of culture.Keywords: bauxite, economical fish, growth, survival rate, tailing ponds
The Utilization of Native Freshwater Mussel <i>Pilsbryoconcha exilis</i> as Biocontrol of Pathogenic Bacteria <i>Aeromonas hydrophila</i> in Tilapia Aquaculture
Authors: Rahman Rahman , Tantio Filibertus , Yuhana Munti , Firdausi Amalia Putri , Sumadi Ranta , Sumadikarta Adna 
Published: August 2020

This research aims to evaluate the capacity of freshwater mussel Pilsbryoconcha exilis as a biocontrol agent to prevent the transmission of Aeromonas hydrophila in tilapia cultivation. Briefly, 10 tilapia fish with average bodyweight 7,88±0,25 g were subjected to four treatments in the 8-liter aquarium with three replications in a randomized design trial. The treatments were M1 (one mussel for a two-liter of water), M2 (two mussels for a two-liter of water), and two control treatments without mussel ( M+ and M-). All treatments, except the M-, then challenged by adding A. hydrophila live culture to obtain a final density of 105 CFU mL-1 into the aquarium for 7 days duration. The final survival rate of fish, the water-total bacterial count, and the blood profile of animals were assessed. The research revealed that there is a significant impact from the presence of freshwater mussel on tilapia cultivation. Generally, the M2 treatment showed better results with a significant different (P<0,05) according to the survival rate of fish (100±0,00%), water-total bacterial count (4,53±0,03 log CFU mL-1), and fish leucocytes (4,30±0,70x104 cell mm-3). Nonetheless, there was no different (P>0,05) effect on fish erythrocytes among the treatments. Therefore, the feeding activity of freshwater mussel in the water column able to deplete pathogenic bacteria abundance and prevent pathogen transmission along with increasing the survival rate of fish.
Genome-Wide Association Study of Prevalent and Persistent Cervical High-Risk Human Papillomavirus (HPV) Infection
Authors: Adebamowo Sally N. , Adeyemo Adebowale A , Rotimi Charles N , Olaniyan Olayinka , Offiong Richard B. , Adebamowo Clement A 
Published: August 2020

Abstract
Background: Genetic factors may influence the susceptibility to high-risk human papillomavirus (hrHPV) infection and persistence. We conducted the first genome-wide association study (GWAS) to identify variants associated with cervical hrHPV infection and persistence.Methods: Participants were 517 Nigerian women evaluated at baseline and 6 months follow-up visits for HPV. HPV was characterized using SPF10/LiPA25. hrHPV infection was positive if at least one carcinogenic HPV genotype was detected in a sample provided at the baseline visit and persistent if at least one carcinogenic HPV genotype was detected in each of the samples provided at the baseline and follow-up visits. Genotyping was done using the Illumina Multi-Ethnic Genotyping Array (MEGA) and imputation was done using the African Genome Resources Haplotype Reference Panel. Association analysis was done for hrHPV infection (125 cases/392 controls) and for persistent hrHPV infection (51 cases/355 controls) under additive genetic models adjusted for age, HIV status and the first principal component (PC) of the genotypes.Results: The mean (±SD) age of the study participants was 38 (±8) years, 48% were HIV negative, 24% were hrHPV positive and 10% had persistent hrHPV infections. No single variant reached genome-wide significance (p < 5 X 10-8). The top three variants associated with hrHPV infections were intronic variants clustered in KLF12 (all OR: 7.06, p =1.43 x 10-6). The top variants associated with cervical hrHPV persistence were in DAP (OR: 6.86, p =7.15 x 10-8), NR5A2 (OR: 3.65, p =2.03 x 10-7) and MIR365-2 (OR: 7.71, p =2.63 x 10-7) gene regions. Conclusions: This exploratory GWAS yielded suggestive candidate risk loci for cervical hrHPV infection and persistence. The identified loci have biological annotation and functional data supporting their role in hrHPV infection and persistence. Given our limited sample size, larger discovery and replication studies are warranted to further characterize the reported associations.
New vectors in northern Sarawak, Malaysian Borneo, for the zoonotic malaria parasite, Plasmodium knowlesi
Authors: De Joshua Ang Xin , Kadir Khamisah Abdul , Mohamad Dayang Shuaisah Awang , Matusop Asmad , Divis Paul Cliff Simon , Yaman Khatijah , Singh Balbir 
Published: August 2020

Abstract
Background: Plasmodium knowlesi is a significant cause of human malaria in Sarawak, Malaysian Borneo. Only one study has been previously undertaken in Sarawak to identify vectors of P. knowlesi, where Anopheles latens was incriminated as the vector in Kapit, central Sarawak. A study was therefore undertaken to identify malaria vectors in a different location in Sarawak.Methods: Mosquitoes found landing on humans and resting on leaves over a 5-day period at two sites in the Lawas District of northern Sarawak were collected and identified. DNA samples extracted from salivary glands of Anopheles mosquitoes were subjected to nested PCR malaria-detection assays. The small sub-unit ribosomal RNA (SSUrRNA) genes of Plasmodium , and the internal transcribed spacer 2 (ITS2) and mitochondrial cytochrome c oxidase subunit 1 (COI) sequences of the mosquitoes were derived from the Plasmodium -positive samples for phylogenetic analysis.Results: A total of 65 anophelines and 127 culicines were collected. By PCR, six An. balabacensis and five An. donaldi were found to have single P. knowlesi infections while three other An. balabacensis had either single, double or triple infections with P. inui, P. fieldi, P. cynomolgi and P. knowlesi . Phylogenetic analysis of the Plasmodium SSUrRNA genes confirmed 3 An. donaldi and 3 An. balabacensis with single P. knowlesi infections, while 3 other An. balabacensis had two or more Plasmodium species of P. inui, P. knowlesi, P. cynomolgi and some species of Plasmodium that could not be conclusively identified. Phylogenies inferred from the ITS2 and/or COI sequences of An. balabacensis and An. donaldi indicate that they are genetically indistinguishable from An. balabacensis and An. donaldi, respectively, found in Sabah, Malaysian Borneo.Conclusions: Previously An. latens was identified as the vector for P. knowlesi in Kapit, central Sarawak, Malaysian Borneo, and now An. balabacensis and An. donaldi have been incriminated as vectors for zoonotic malaria in Lawas, northern Sarawak.
Presence of heat shock protein 47-positive fibroblasts in cancer stroma is associated with increased risk of postoperative recurrence in patients with lung cancer
Authors: Miyamura Takuto , Sakamoto Noriho , Ishida Kaori , Kakugawa Tomoyuki , Taniguchi Hirokazu , Akiyama Yoshiko , Okuno Daisuke , Hara Atsuko , Kido Takashi , Ishimoto Hiroshi , Miyazaki Takuro , Matsumoto Keitaro , Tsuchiya Tomoshi , Yamaguchi Hiroyuki , Miyazaki Taiga , Obase Yasushi , Ishimatsu Yuji , Nagayasu Takeshi , Mukae Hiroshi 
Published: August 2020

Abstract
Background: Heat shock protein 47 (HSP47), a collagen-binding protein, has a specific role in the intracellular processing of procollagen production. HSP47 expression is associated with cancer growth and metastasis in several types of cancers. However, none of the studies have assessed whether HSP47 expression is associated with the risk of postoperative recurrence of lung cancer until now. Therefore, we aimed to assess this association.Methods: The study population consisted of a cohort of consecutive patients who underwent surgery for lung cancer at Nagasaki University Hospital, Nagasaki, Japan, from January 2009 to December 2010. Patient characteristics, survival and disease-free survival (DFS), and laboratory findings were compared between patients who tested positive and negative for HSP47 expression in lung cancer cells and between those who showed high and low numbers of HSP47-positive fibroblasts in cancer stroma.Results: A total of 133 patients underwent surgery for lung cancer. Sixty-seven patients (48.5%) had HSP47-positive cancer cells, and 91 patients (68.4%) had a higher number of HSP47-positive fibroblasts. The patients with a high number of HSP47-positive fibroblasts had a shorter DFS than those with a low number of HSP47-positive fibroblasts. Multivariate analysis identified only the presence of a high number of HSP47-positive fibroblasts as an independent risk factor for recurrence of lung cancer after surgery (odds ratio, 4.371; 95% confidence interval, 1.054–29.83; P = 0.042)Conclusion: The present study demonstrated that the presence of a high number of HSP47-positive fibroblasts in the cancer stroma was a risk factor for recurrence of lung cancer after surgery.
Genetic polymorphism and natural selection of circumsporozoite protein in Myanmar Plasmodium vivax
Authors: Võ Tuấn Cường , Lê Hương Giang , Kang Jung-Mi , Moe Mya , Naw Haung , Myint Moe Kyaw , Lee Jinyoung , Sohn Woon-Mok , Kim Tong-Soo , Na Byoung-Kuk 
Published: August 2020

Abstract
Background: Circumsporozoite surface protein (CSP) of malaria parasites has been recognized as one of the leading vaccine candidates. Clinical trials of vaccines for vivax malaria incorporating Plasmodium vivax CSP (PvCSP) have demonstrated their effectiveness in preventing malaria, at least in part. However, genetic diversity of PvCSP in the natural population is still a major concern.

Methods: A total of 171 blood samples collected from patients infected with Plasmodium vivax in Myanmar analysed in this study. The gene for PvCSP was amplified by polymerase chain reaction, followed by T&A cloning and sequencing. Polymorphic characteristics and natural selection of Myanmar PvCSP population in Myanmar were analysed using DNASTAR, MEGA6 and DnaSP programs. The polymorphic pattern and natural selection of publicly accessible global PvCSP sequences were also comparatively analysed.

Results: Myanmar PvCSP sequences were divided into two subtypes VK210 and VK247 comprising 143 and 28 sequences, respectively. The VK210 subtypes showed higher levels of genetic diversity and polymorphism than the VK247 subtypes. The N-terminal non-repeat region of PvCSP displayed limited genetic variations in the global population. Different patterns of octapeptide insertion (ANKKAEDA in VK210 and ANKKAGDA in VK247) and tetrapeptide repeat motif (GGNA) were identified in the C-terminal region of global PvCSP population. Meanwhile, the central repeat region (CRR) of Myanmar and global PvCSP, both in VK210 and VK247 variants, was highly polymorphic. The high level of genetic diversity in the CRR has been attributed to the different numbers, types and combinations of peptide repeat motifs (PRMs). Interestingly, 27 and 5 novel PRMs were found in Myanmar VK210 and VK247 variants, respectively.

Conclusion: Comparative analysis of the global PvCSP population suggests a complex genetic profile of PvCSP in the global population. These results widen understanding of the genetic make-up of PvCSP in the global P. vivax population and provide valuable information for the development of a vaccine based on PvCSP.
Patterns of food parenting practices regarding junk food and sugary drinks among parent-child dyads
Authors: Thomson Jessica , Hennessy Erin , Landry Alicia , Goodman Melissa 
Published: August 2020

Abstract
Background

Children’s food preference and intake patterns are affected by parental child feeding practices. The objective was to determine patterns of food parenting practices regarding junk food and sugary drinks (JS) and investigate their associations with demographic characteristics and dietary intake in a large cohort of parents and their children (12-17 years).

Methods

Dyadic survey data from the cross-sectional, internet-based Family Life, Activity, Sun, Health, and Eating Study, conducted in 2014, were analyzed using latent class analysis to identify patterns of use for six JS parenting practices – negative emotions, restriction, monitoring, availability, modeling, and child involvement – based on parent and child report. Model covariates included self-reported parent and child sex, age (child only), body mass index category (based on height and weight), added sugars intake, and legitimacy of parental authority.

Results

Based on 1,657 parent-child dyads, five parenting practice patterns were identified representing different levels of practice use – Complete Influencers (28%; reference class), Indifferent Influencers (21%), Negative Influencers (20%), Minimal Influencers (18%), and Disagreeing Influencers (13%). Compared to older child dyads, younger child dyads were less likely to belong to Indifferent and Minimal Influencers (79% and 63% lower odds, respectively). Greater parent added sugars intake increased the odds of belonging to Indifferent and Negative Influencers (4% and 5% higher for every teaspoon increase, respectively) while greater child added sugars intake decreased the odds of belonging to Minimal Influencers (6% lower for every teaspoon increase). Compared to dyads with high scores, dyads with low child scores for legitimacy of parental authority regarding JS were 18 times as likely to belong to Disagreeing Influencers.

Conclusions

The study findings suggest that parents utilize distinct patterns of feeding practices regarding JS ranging from use of many practices, use of some practices, to low use of any practice, with differential associations with parent and child intakes of added sugars. Counseling or intervening with parents to use a mix of structure practices, such as availability and modeling, to positively influence their child’s and possibly their own intake of sugary snacks and drinks may prove more efficacious than use of coercive control practices, such as negative emotions.