bluuebunny/crossref_metadata_2025_split huggingface dataset:
Latent Blowout of COVID-19 Globally: An Effort to Healthcare Alertness via Medical GIS Approach
Authors: Sharma Laxmi Kant , Verma Rajani Kant 
Published: May 2020

AbstractSince January 2020, the COVID-19 pandemic has been escalating from North America to Asia. Various studies projected the spread of pandemic globally, using air passenger data from an infected area. But there could be various parameters that can be the basis for the forecasting of the pandemic. Current research adopts the Medical GIS approach and incorporates critical parameters from various domains to create a global alertness scale to combat the pandemic. The finding of the study ranks the countries on a 1 to 9 scale based on the spatial alertness In this context, the study focuses on the role of GIS techniques as an enabler to fight against the global pandemic and could be beneficial for the authorities to adopt timely preventive actions.One Sentence SummaryGlobal Alertness Ranking to combat COVID-19
Applications and barriers to use of an mHealth iPhone application for self-management of chronic recurrent medical conditions: A Pilot Study
Authors: Mande Archana , Moore Susan L. , Banaei-Kashani Farnoush , Kaizer Alexander M. , Echalier Benjamin , Bull Sheana , Rosenberg Michael A. 
Published: May 2020

AbstractManagement of chronic recurrent medical conditions (CRMC), such as migraine headaches, chronic pain and anxiety/depression, is a major challenge for modern providers. The fact that often the most effective treatments and/or preventative measures for CRMCs vary from patient to patient lends itself to a platform for self-management by patients. However, to develop such an mHealth app requires an understanding of the various applications, and barriers, to real-world use. In this pilot study with internet-based recruitment, we conducted an assessment of user satisfaction of the iMTracker iOS (iPhone) application for CRMC self-management through a self-administered survey of subjects with CRMCs. From May 15, 2019 until March 27, 2020, we recruited 135 subjects to pilot test the iMTracker application for user-selected CRMCs. The most common age group was 31–45 (48.2%), followed by under 30 (22.2%) and 46–55 (20%). There were no subjects over 75 years old completing the survey. 38.8% of subjects were college graduates, followed by 29.6% with a Master’s degree, and 25.9% with some college. No subjects had not graduated from high school, and only 2 (1.5%) did not attend college after high school. 80.7% of subjects were self-identified as Caucasian, and 90.4% as not Hispanic or Latino. The most common CRMC was pain (other than headaches) in 40% of subjects, followed by mental health in 17.8% and headaches in 15.6%. 39.3% of subjects experienced the condition multiple times in a day, 40.0% experienced the condition daily, and 14.8% experienced the condition weekly, resulting in a total of 94.1% of subjects experiencing the condition at least weekly. Among the concerns about a self-management app, time demands (54.8%) and ineffectiveness (43.7%) were the most prominent, with privacy (24.4%) and data security (25.2%) also noted. In summary, we found internet-based recruitment identified primarily Caucasian population of relatively young patients with CRMCs of relatively high recurrence rate. Future work is needed to examine the use of this application in older, underrepresented minorities, and lower socioeconomic status populations.
Can Nebulised Heparin Reduce Time to Extubation in SARS-CoV-2 (CHARTER Study) – Protocol
Authors: Dixon Barry , Smith Roger J , Artigas Antonio , Laffey John , McNicholas Bairbre , Schmidt Eric , Nunes Quentin , Skidmore Mark , Lima Marcelo Andrade de , Moran John L , VanHaren Frank , Doig Gordon , Ghosh Angajendra , Said Simone , Gupta Sachin , Santamaria John D 
Published: May 2020

AbstractIntroductionCOVID-19 is associated with the development of ARDS displaying the typical features of diffuse alveolar damage with extensive pulmonary coagulation activation resulting in fibrin deposition in the microvasculature and formation of hyaline membranes in the air sacs. The anti-coagulant actions of nebulised heparin limit fibrin deposition and progression of lung injury. Serendipitously, unfractionated heparin also inactivates the SARS-CoV-2 virus and prevents its entry into mammalian cells. Nebulisation of heparin may therefore limit both fibrin-mediated lung injury and inhibit pulmonary infection by SARS-CoV-2. For these reasons we have initiated a multi-centre international trial of nebulised heparin in patients with COVID-19.Methods and interventionMechanically ventilated patients with confirmed or strongly suspected SARS-CoV-2 infection, hypoxaemia and an acute pulmonary opacity in at least one lung quadrant on chest X-ray, will be randomised to nebulised heparin 25,000 Units every 6 hours or standard care for up to 10 days while mechanically ventilated. The primary outcome is the time to separation from invasive ventilation to day 28, where non-survivors to day 28 are treated as though not separated from invasive ventilation.Ethics and disseminationThe study protocol has been submitted to the human research and ethics committee of St Vincent’s Hospital, Melbourne, Australia. Submission is pending in other jurisdictions. Results of this study will be published in scientific journals and presented at scientific meetings.Trial RegistrationACTRN: 12620000517976
Regulation of bacterial cell cycle progression by redundant phosphatases
Authors: Coppine Jérôme , Kaczmarczyk Andreas , Petit Kenny , Brochier Thomas , Jenal Urs , Hallez Régis 
Published: May 2020

AbstractIn the model organismCaulobacter crescentus, a network of two-component systems involving the response regulators CtrA, DivK and PleD coordinate cell cycle progression with differentiation. Active phosphorylated CtrA prevents chromosome replication in G1 cells while simultaneously regulating expression of genes required for morphogenesis and development. At the G1-S transition, phosphorylated DivK (DivK~P) and PleD (PleD~P) accumulate to indirectly inactivate CtrA, which triggers DNA replication initiation and concomitant cellular differentiation. The phosphatase PleC plays a pivotal role in this developmental program by keeping DivK and PleD phosphorylation levels low during G1, thereby preventing premature CtrA inactivation. Here, we describe CckN as a second phosphatase akin to PleC that dephosphorylates DivK~P and PleD~P in G1 cells. However, in contrast to PleC, we do not detect kinase activity with CckN. The effects of CckN inactivation are largely masked when PleC is present, but become evident when PleC and DivJ, the major kinase for DivK and PleD, are absent. Accordingly, mild overexpression ofcckNrestores most phenotypic defects of apleCnull mutant. We also show that CckN and PleC are proteolytically degraded in a ClpXP-dependent way well before the onset of the S phase. Surprisingly, known ClpX adaptors are dispensable for PleC and CckN proteolysis, suggesting the existence of adaptors specifically involved in proteolytic removal of cell cycle regulators. SincecckNexpression is induced in stationary phase, depending on the stress alarmone (p)ppGpp, we propose that CckN acts as an auxiliary factor responding to environmental stimuli to modulate CtrA activity under suboptimal conditions.ImportanceTwo-component signal transduction systems are widely used by bacteria to sense environmental signals and respond accordingly by modulating various cellular processes, such as cell cycle progression. InCaulobacter crescentus, PleC acts as a phosphatase that indirectly protects the response regulator CtrA from premature inactivation during the G1 phase of the cell cycle. Here, we provide genetic and biochemical evidence that PleC is seconded by another phosphatase, CckN. The activity of PleC and CckN phosphatases is restricted to G1 phase since both proteins are timely degraded by proteolysis just before the G1-S transition. This degradation requires new proteolytic adaptors as well as an unsuspected N-terminal motif for CckN. Our work illustrates a typical example of redundant functions between two-component proteins.
Reproductive biology of wild and domesticated Ensete ventricosum: Further evidence for maintenance of sexual reproductive capacity in a vegetatively propagated perennial crop
Authors: Tamrat Solomon , Borrell James S. , Shiferaw Eleni , Wondimu Tigist , Kallow Simon , Davies Rachael M. , Dickie John B. , Nuraga Gizachew W. , White Oliver , Woldeyes Feleke , Demissew Sebsebe , Wilkin Paul 
Published: May 2020

AbstractLoss of sexual reproductive capacity has been proposed as a syndrome of domestication in vegetatively propagated crops, but there are relatively few examples from agricultural systems. In this study we compare sexual reproductive capacity in wild (sexual) and domesticated (vegetative) populations of enset (Ensete ventricosum (Welw.) Cheesman), a tropical banana relative and Ethiopian food security crop.We examined floral and seed morphology and germination ecology across 35 wild and domesticated enset. We surveyed variation in floral and seed traits, including seed weight, viability and internal morphology, and germinated seeds across a range of constant and alternating temperature regimes to characterise optimum germination requirements.We report highly consistent floral allometry, seed viability, internal morphology and days to germination in wild and domesticated enset. However, seeds from domesticated plants responded to cooler temperatures with greater diurnal range. Shifts in germination behaviour appear concordant with a climatic envelope shift in the domesticated distribution.Our findings provide evidence that sexual reproductive capacity has been maintained despite long-term near-exclusive vegetative propagation in domesticated enset. Furthermore, certain traits such as germination behaviour and floral morphology, may be under continued selection, presumably through rare sexually reproductive events. Compared to sexually propagated crops banked as seeds, vegetative crop diversity is typically conserved in living collections that are more costly and insecure. Improved understanding of sexual propagation in vegetative crops may have applications in germplasm conservation and plant breeding.
Measuring Sequences of Representations with Temporally Delayed Linear Modelling
Authors: Liu Yunzhe , Dolan Raymond J , Penagos-Vargas Hector Luis , Kurth-Nelson Zeb , Behrens Timothy 
Published: May 2020

SUMMARYThere are rich structures in off-task neural activity. For example, task related neural codes are thought to be reactivated in a systematic way during rest. This reactivation is hypothesised to reflect a fundamental computation that supports a variety of cognitive functions. Here, we introduce an analysis toolkit (TDLM) for analysing this activity. TDLM combines nonlinear classification and linear temporal modelling to testing for statistical regularities in sequences of neural representations. It is developed using non-invasive neuroimaging data and is designed to take care of confounds and maximize sequence detection ability. The method can be extended to rodent electrophysiological recordings. We outline how TDLM can successfully reveal human replay during rest, based upon non-invasive magnetoencephalography (MEG) measurements, with strong parallels to rodent hippocampal replay. TDLM can therefore advance our understanding of sequential computation and promote a richer convergence between animal and human neuroscience research.
Priming of MSCs with inflammation-relevant signals affects extracellular vesicle biogenesis, surface markers, and modulation of T cell subsets
Authors: Andrews Seth , Maughon Ty , Marklein Ross , Stice Steven 
Published: May 2020

AbstractAlthough considerable evidence exists supporting the use of mesenchymal stromal cells (MSCs) for treating immune diseases, successful clinical translation has been challenging and has led researchers to investigate cell-free alternatives. MSC-derived extracellular vesicles (MSC-EVs) have been shown to mediate a significant portion of the observed therapeutic effect, including immunosuppression. MSCs have been shown to respond to different aspects of the injury microenvironment such as inflammatory cytokines and hypoxia, although acidosis has not been investigated and different conditions have not been assessed in terms of their effects on MSC-EV function. This study investigated the effects of acidosis, hypoxia, and inflammatory cytokine priming on MSCs and MSC-EVs. We cultured MSCs in the presence of acidosis, hypoxia, or inflammatory cytokines (Interferon-gamma and Tumor Necrosis Factor-alpha) and compared the characteristics of their EVs as well as their uptake by and suppression of different T cell subsets. MSCs showed a greater effect on suppressing activated CD4+and CD8+T cells than MSC-EVs. However, MSC-EVs from MSCs primed with acidosis increased CD4+and CD8+regulatory T cell frequency in vitro. This functional response was reflected by MSC-EV uptake. MSC-EVs from acidosis-primed MSCs were taken up by CD4+and CD8+regulatory T cells at a significantly higher level than MSC-EVs from control, hypoxic, and inflammatory cytokine groups. These data suggest that a simple low-cost alteration in MSC culture conditions, acidosis, can generate extracelluar vesicles that have a desirable influence on anti inflammatory T cell subtypes.
Decision making in auditory externalization perception: model predictions for static conditions
Authors: Baumgartner Robert , Majdak Piotr 
Published: May 2020

AbstractUnder natural conditions, listeners perceptually attribute sounds to external objects in their environment. This core function of perceptual inference is often distorted when sounds are produced via hearing devices such as headphones or hearing aids, resulting in sources being perceived unrealistically close or even inside the head. Psychoacoustic studies suggest a mixed role of various monaural and interaural cues contributing to the externalization process. We developed a model framework for perceptual externalization able to probe the contribution of cue-specific expectation errors and to contrast dynamic versus static strategies for combining those errors within static listening environments. Effects of reverberation and visual information were not considered. The model was applied to various acoustic distortions as tested under various spatially static conditions in five previous experiments. Most accurate predictions were obtained for the combination of monaural and interaural spectral cues with a fixed relative weighting (approximately 60% of monaural and 40% of interaural). That model version was able to reproduce the externalization rating of the five experiments with an average error of 12% (relative to the full rating scale). Further, our results suggest that auditory externalization in spatially static listening situations underlie a fixed weighting of monaural and interaural spectral cues, rather than a dynamic selection of those auditory cues.
Transcriptional control of DNA repair networks by CDK7 regulates sensitivity to radiation in Myc-driven Medulloblastoma
Authors: Veo Bethany , Danis Etienne , Pierce Angela , Wang Dong , Fosmire Susan , Sullivan Kelly D. , Joshi Molishree , Khanal Santosh , Dahl Nathan , Karam Sana , Serkova Natalie , Venkataraman Sujatha , Vibhakar Rajeev 
Published: May 2020

AbstractMyc-driven Medulloblastoma remains a major therapeutic challenge due to frequent metastasis and a poor 5-year survival rate. Myc gene amplification results in transcriptional dysregulation, proliferation, and survival of malignant cells. To identify therapeutic targets in Myc-amplified medulloblastoma we performed a CRISPR-Cas9 essentiality screen targeting 1140 genes annotated as the druggable genome. CDK7 was identified as a mediator of medulloblastoma tumorigenesis. Using covalent inhibitors and genetic depletion of CDK7 we observe the cessation of tumor growth in xenograft mouse models and increase in apoptotic mechanisms. The results are attributed to repression of a core set of Myc-driven transcriptional programs mediating DNA repair. We further establish that blocking CDK7 activity sensitizes cells to ionizing radiation leading to accrual of DNA damage and extended survival and tumor latency in medulloblastoma xenograft mouse models. Our studies establish a mechanism for selective inhibition of Myc-driven MB by CDK7 inhibition combined with radiation as a viable therapeutic strategy for Myc-amplified medulloblastoma.
Trait-based responses to land use and canopy dynamics modify long-term diversity changes in forest understories
Authors: Happonen Konsta , Muurinen Lauralotta , Virtanen Risto , Kaakinen Eero , Grytnes John-Arvid , Kaarlejärvi Elina , Parisot Philippe , Wolff Matias , Maliniemi Tuija 
Published: May 2020

AbstractAimLand use is the foremost cause of global biodiversity decline, but species do not respond equally to land-use practices. Instead, it is suggested that responses vary with species traits, but long-term data on the trait-mediated effects of land-use on communities is scarce. Here we study how forest understory communities have been affected by two land-use practices during 4–5 decades, and whether changes in plant diversity are related to changes in functional composition.LocationFinlandTime period1968–2019Major taxa studiedVascular plantsMethodsWe resurveyed 245 vegetation plots in boreal herb-rich forest understories, and used hierarchical Bayesian linear models to relate changes in diversity, species composition, average plant size, and leaf economic traits to reindeer abundance, forest management intensity, and changes in climate, canopy cover and composition.ResultsIntensively managed forests decreased in species richness and had increased turnover, but management did not affect functional composition. Increased reindeer densities corresponded with increased leaf dry matter content, evenness and diversity, and decreased height and specific leaf area. Successional development in the canopy was associated with increased specific leaf area and decreased leaf dry matter content and height in the understory over the study period. Effects of reindeer abundance and canopy density on diversity were partially mediated by vegetation height, which had a negative relationship with evenness. Observed changes in climate had no discernible effect on any variable.Main conclusionsFunctional traits are useful in connecting vegetation changes to the mechanisms that drive them, and provide unique information compared to turnover and diversity metrics. These trait-dependent selection effects could inform whether species benefit or suffer from land use changes and explain observed biodiversity changes under global change.
Estimating Movement Smoothness from Inertial Measurement Units
Authors: Melendez-Calderon Alejandro , Shirota Camila , Balasubramanian Sivakumar 
Published: May 2020

AbstractThere is an increasing trend in using inertial measurement units (IMUs) to estimate movement quality and quantity, and infer the nature of motor behavior. The current literature contains several attempts to estimate movement smoothness using data from IMUs, most of which assume that the translational and rotational kinematics measured by IMUs can be directly used with existing smoothness measures - spectral arc length (SPARC) and log dimensionless jerk (LDLJ-V). However, there has been no investigation of the validity of these approaches. In this paper, we systematically evaluate the appropriateness of the using these measures on the kinematics measured by an IMU. We show that: (a) current measures (SPARC and LDLJ-V) are inappropriate for translational movements; and (b) SPARC and LDLJ-V can be used rotational kinematics measured by an IMU. For discrete translational movements, we propose a modified version of the LDLJ-V measure, which can be applied to acceleration data (LDLJ-A), while roughly maintaining the properties of the original measure. However, accuracy of LDLJ-A depends on the IMU orientation estimation error. We evaluate the performance of these measures using simulated and experimental data. We then provide recommendations for how to appropriately apply these measures in practice, and the various factors to be aware of when performing smoothness analysis using IMU data.
Platinum-Induced Ubiquitination of Phosphorylated H2AX by RING1A is Mediated by Replication Protein A in Ovarian Cancer
Authors: Sriramkumar Shruthi , Matthews Timothy D. , Ghobashi Ahmed H. , Miller Samuel A. , VanderVere-Carozza Pamela S. , Pawelczak Katherine S. , Nephew Kenneth P. , Turchi John J. , O’Hagan Heather M. 
Published: May 2020

AbstractPlatinum resistance is a common occurrence in high grade serous ovarian cancer (HGSOC) and a major cause of OC deaths. Platinum agents form DNA crosslinks, which activate nucleotide excision repair (NER), fanconi anemia (FA) and homologous recombination repair (HRR) pathways. Chromatin modifications occur in the vicinity of DNA damage and play an integral role in the DNA damage response (DDR). Chromatin modifiers, including polycomb repressive complex 1 (PRC1) members, and chromatin structure are frequently dysregulated in OC and can potentially contribute to platinum resistance. However, the role of chromatin modifiers in the repair of platinum DNA damage in OC is not well understood. We demonstrate that the PRC1 complex member RING1A mediates monoubiquitination of lysine 119 of phosphorylated H2AX (γH2AXub1) at sites of platinum DNA damage in OC cells. After platinum treatment, our results reveal that NER and HRR both contribute to RING1A localization and γH2AX monoubiquitination. Importantly, replication protein A (RPA), involved in both NER and HRR, mediates RING1A localization to sites of damage. Furthermore, RING1A deficiency impaired the activation of the G2/M DNA damage checkpoint and reduced the ability of OC cells to repair platinum DNA damage. Elucidating the role of RING1A in the DDR to platinum agents will allow for the identification of therapeutic targets to improve the response of OC to standard chemotherapy regimens.