bluuebunny/crossref_metadata_2025_split huggingface dataset:
Potential of herbaceous vegetation as animal feed in semi‐arid Mediterranean saline environments: The case for Tunisia
Authors: Hessini Kamel , Jeddi Kaouthar , Shaer H.M El , Smaoui Abderrazak , Salem Hichem Ben , Siddique Kadambot H.M 
Published: February 2020

AbstractWith the depletion in arable land due to climate change, the use of naturally salt‐tolerant species to provide forage resources in arid and saline environments has become an emerging strategy. This study surveyed and determined the nutritive value of selected Tunisian vegetative species for use in Mediterranean saline environments. Eighteen wild species were analyzed for their nutrient contents and antinutritional factors. Chenopodiaceae accumulated more NaCl than Poaceae. Arthrocnemum indicum had the highest crude protein (CP) content, and Stipa roterta had the lowest. Poaceae had the highest fiber constituents (neutral and acid) and the lowest oxalate contents. Daily net gas production (GP) in the Chenopodiaceae and Poaceae ranged from 9.5 to 14.5 and from 13.8 to 37.9 ml per 0.2 mg dry matter (DM), respectively. Total phenol content and organic matter digestibility were species dependent. The metabolizable energy (ME) in the tested species ranged from 4.6 to 9.4 MJ kg−1 DM, with Catapodium rigidum and Arthrocnemum indicum having the lowest and highest ME values, respectively. In crux, Chenopodiaceae had the highest NaCl, CP, ME, and oxalate contents but had the lowest fiber and net GP. Sodium chloride content was positively correlated with CP and oxalate and negatively correlated with net GP. In arid and saline environments, where high salinity prevails and fodder resources are scarce, salt‐tolerant plants such as Suaeda fruticosa, Arthrocnemum indicum, and Halocnemum strobilaceum could be included in ruminant feeding calendars.
Management practices for optimal kikuyugrass quality and playing conditions
Authors: Schiavon Marco , Mock Tyler , Stowell Larry J. , Baird J. H. 
Published: February 2020

AbstractKikuyugrass [Cenchrus clandestinus (Hochst. ex Chiov.) Morrone] is considered either an invasive weed or the desired species on many golf courses, athletic fields, and other turf areas along coastal and inland California. A field study was conducted at the University of California, Riverside in 2012 and 2013 on ‘Whittet’ kikuyugrass mowed at 11 mm to identify management practices for producing sufficient turf quality and optimal playing conditions for golf course fairways and athletic fields. The study evaluated the effect of mowing frequency (three vs. six times per week), cultivation practice (verticutting vs. grooming), applications of trinexapac‐ethyl (TE), and nitrogen fertilization rates (96 vs. 240 kg N ha−1 yr−1) on turf quality, Normalized Difference Vegetation Index (NDVI), turf firmness, ball roll distance (BRD), and tensile strength. Mowing six times per week produced the highest turf quality turf during summer when kikuyugrass grows most vigorously, while trends were not clear for cultivation practice effects on turf visual quality. However, verticutting twice per year produced the least scalped and firmest turf, while decreasing BRD. Application of TE increased turf quality, NDVI, and BRD, but decreased tensile strength of the sward. Slightly higher turf quality was detected in plots fertilized at 240 kg N ha−1 yr−1 compared to those fertilized with the low N rate (6.0 vs. 5.8), but results do not seem to justify higher N fertility. Overall, results demonstrated that kikuyugrass available in California could benefit from high input management practices such as TE applications, high mowing frequency, and verticutting.
Life cycle assessment and cost evaluation of emerging technologies at early stages: The case of continuous flow synthesis of Rufinamide
Authors: Grimaldi Fabio , de Leon Izeppi Gerardo A. , Kirschneck Dirk , Lettieri Paola , Escribà‐Gelonch Marc , Hessel Volker 
Published: February 2020

AbstractIn the pharma and fine chemical industries, the development of continuous flow technologies is a process intensification step of primary importance towards the manufacturing of high‐quality products, while reducing the environmental impact and cost of production. The sustainability and profitability of a process can be measured through life cycle Assessment and cost evaluation. However, when applied to emerging technologies, these need to be performed at different stages of the process development in order to limit the uncertainties arising from the scale‐up, and hence providing high‐fidelity projections of environmental impacts and costs at larger scales. The output of the assessment can in fact vary significantly depending on the maturity of the technology and this translates into having different results at commercial scale compared to early estimations. Therefore, in this article, we perform an assessment at two different scales of production, lab and mini‐pilot scale, with the aim of quantifying the uncertainties of the assessment related to the scale‐up, identifying the hotspots of the system, and hence providing guidelines for the further steps of process development. The subject of the assessment is the continuous flow synthesis of Rufinamide. It is the first time that this synthesis is evaluated at pilot‐scale. The results show that low yields in the cycloaddition drastically affect the waste management and the production of precursors, and hence increases environmental impacts and cost of production. This calls for the need of prioritizing the optimization of this synthesis step in order to deploy a green and economically competitive production technology.
Introducing a novel chemotherapeutic drug formulated by iron nanoparticles for the clinical trial studies
Authors: Ma Lei , Ahmeda Ahmad , Wang Kaifeng , Jalalvand Ali R. , Sadrjavadi Komail , Nowrozi Masoumeh , Zangeneh Akram , Zangeneh Mohammad Mahdi , Wang Xiaojing 
Published: February 2020

AbstractIn recent years, the experiments have revealed that plants raise the cytotoxicity and anticancer potentials of iron nanoparticles against several cell lines especially tumor cell lines. In the present study, we formulated a modern drug by iron nanoparticles (FeNPs) containing Glycyrrhiza glabra L leaf for the treatment of acute lymphoblastic leukemia. Characterization of FeNPs was done by TEM, FE‐SEM, UV–Vis, and FT‐IR. FE‐SEM and TEM images revealed an average diameter of 11–18 nm for the nanoparticles. UV–Vis revealed an absorption band at 298 nm that is related to the surface plasmon resonance of FeNPs. FT‐IR results offered antioxidant compounds in the nanoparticles were the sources of reducing power, reducing iron ions to FeNPs. MTT assay was used on normal (HUVEC) and acute lymphoblastic leukemia (MOLT‐3, CEM/C2, TALL‐104, and CCRF‐CEM) cell lines for comparing the anti‐acute lymphoblastic leukemia properties of FeCl3, G. glabra, and FeNPs. Iron nanoparticles had very low cell viability dose‐dependently against MOLT‐3, CEM/C2, TALL‐104, CCRF‐CEM cell lines without any cytotoxicity on the HUVEC cell line. The best result of cytotoxicity property of FeNPs against the above cell lines was seen in the case of CCRF‐CEM cell line. These nanoparticles can be administrated in humans for the treatment of acute lymphoblastic leukemia after confirming in the in vivo and clinical trials.
Development of 32 novel microsatellite loci in Juglans sigillata using genomic data
Authors: Xu Zu‐Chang , Jin Ye‐Chuan , Milne Richard I. , Xiahou Zuo‐Ying , Qin Han‐Tao , Ye Lin‐Jiang , Gao Lian‐Ming , Liu Jie , Li De‐Zhu 
Published: February 2020

PremiseA novel set of microsatellite markers was developed for Juglans sigillata (Juglandaceae), an endemic walnut species in southwestern China, to facilitate cultivar identification and future investigations into the genetic structure and domestication history of this species and its close relatives.Methods and ResultsWe developed 32 microsatellite loci for J. sigillata using genomic data and used them to examine 60 individuals from three natural populations. A high level of polymorphism was detected by these primers, with up to eight alleles observed per locus, and an average of four alleles across populations. The levels of observed and expected heterozygosity ranged from 0.000–1.000 and 0.000–0.785, respectively. All but two of the loci were also successfully amplified in three closely related Eurasian Juglans species (J. regia, J. cathayensis, and J. mandshurica).ConclusionsThe microsatellite loci identified here provide a powerful resource for examining the genetic structure and domestication history of Juglans, as well as identification of its cultivars.
Demarcating spectrums of predatory publishing: Economic and institutional sources of academic legitimacy
Authors: Siler Kyle 
Published: February 2020

AbstractThe emergence of open access (OA) publishing has altered incentives and opportunities for academic stakeholders and publishers. These changes have yielded a variety of new economic and academic niches, including journals with questionable peer‐review systems and business models, commonly dubbed “predatory publishing.” Empirical analysis of Cabellʼs Journal Blacklist reveals substantial diversity in types and degrees of predatory publishing. While some blacklisted publishers produce journals with many severe violations of academic norms, “gray” journals and publishers occupy borderline or ambiguous niches between predation and legitimacy. Predation in academic publishing is not a simple binary phenomenon and should instead be perceived as a spectrum with varying types and degrees of illegitimacy. Conceptions of predation are based on overlapping evaluations of academic and economic legitimacy. High institutional status benefits publishers by reducing conflicts between—if not aligning—professional and market institutional logics, which are more likely to conflict and create illegitimacy concerns in downmarket niches. High rejection rates imbue high‐status journals with value and pricing power, while low‐status OA journals face “predatory” incentives to optimize revenue via low selectivity. Status influences the social acceptability of profit‐seeking in academic publishing, rendering lower‐status publishers vulnerable to being perceived and stigmatized as illegitimate.
Observation of Interfacial Degradation of Li6PS5Cl against Lithium Metal and LiCoO2 via In Situ Electrochemical Raman Microscopy
Authors: Zhou Yundong , Doerrer Christopher , Kasemchainan Jitti , Bruce Peter G. , Pasta Mauro , Hardwick Laurence J. 
Published: February 2020

AbstractSulfide‐based Li+ conducting solid electrolytes, such as argyrodite, Li6PS5Cl, for all‐solid‐state batteries can have comparable ionic conductivities with liquid electrolytes. However, the interface between sulfide containing solid electrolytes and Li metal and Li‐ion positive electrodes has been found to be unstable, leading to poor cell performance and cycling. Understanding the in situ evolution of interfacial layers between the electrolyte and both electrodes is of paramount importance for designing stable and long‐life solid‐state batteries. Here, in situ Raman microscopy was used to study the interface between Li6PS5Cl electrolyte and metallic Li and LiCoO2. Under potential control, Raman microscopy identified the appearance of degradation products (Li2S, P2Sx and polysulfides) at the electrode/solid electrolyte interface.
LC–MS/MS determination of buparlisib, a phosphoinositide 3 kinase inhibitor in rat plasma: Application to a pharmacokinetic study
Authors: Wang Jingyun , Ming Meng , Yu Lin , Chen Guangliang , Hao Nan , Meng Yuqi , Fang Lina 
Published: February 2020

AbstractBuparlisib is a selective phosphoinositide 3 kinase inhibitor currently evaluated in clinical trials. We developed and validated an LC–MS/MS coupled with a one‐step protein precipitation extraction method for the quantitation of buparlisib in rat plasma. After protein precipitation with acetonitrile, the plasma sample was analyzed using a Cortecs UPLC C18 column, with acetonitrile–0.1% formic acid as the mobile phase system. Mass spectrometric detection was conducted in positive ionization mode, with target quantitative ion pair of m/z 411.2 → 367.2 for buparlisib. The calibration curve showed good linearity (1.0–3000 ng/ml), with acceptable accuracy (RE ranging from −6.2 to 5.9%) and precision (RSD within 8.2%) values at quality control concentrations. Extraction recovery from plasma was 80.9–88.7% and the matrix effect was negligible (92.6–95.2%). The validated method presented a simple quantification method of buparlisib in detail and utilized it for a pharmacokinetic study at three dose concentrations after oral administration in Wistar rats.
MicroRNA‐based recombinant AAV vector assembly improves efficiency of suicide gene transfer in a murine model of lymphoma
Authors: Khan Nusrat , Cheemadan Sabna , Saxena Himanshi , Bammidi Sridhar , Jayandharan Giridhara R. 
Published: February 2020

AbstractRecent success in clinical trials with recombinant Adeno‐associated virus (AAV)‐based gene therapy has redirected efforts in optimizing AAV assembly and production, to improve its potency. We reasoned that inclusion of a small RNA during vector assembly, which specifically alters the phosphorylation status of the packaging cells may be beneficial. We thus employed microRNAs (miR‐431, miR‐636) identified by their ability to bind AAV genome and also dysregulate Mitogen‐activated protein kinase (MAPK) signaling during vector production, by a global transcriptome study in producer cells. A modified vector assembly protocol incorporating a plasmid encoding these microRNAs was developed. AAV2 vectors packaged in the presence of microRNA demonstrated an improved gene transfer potency by 3.7‐fold, in vitro. Furthermore, AAV6 serotype vectors encoding an inducible caspase 9 suicide gene, packaged in the presence of miR‐636, showed a significant tumor regression (~2.2‐fold, P < .01) in a syngeneic murine model of T‐cell lymphoma. Taken together, we have demonstrated a simple but effective microRNA‐based approach to improve the assembly and potency of suicide gene therapy with AAV vectors.
Treatment optimization for recurrent hepatocellular carcinoma: Repeat hepatic resection versus radiofrequency ablation
Authors: Lu Liang‐He , Mei Jie , Kan Anna , Ling Yi‐Hong , Li Shao‐Hua , Wei Wei , Chen Min‐Shan , Zhang Yong‐Fa , Guo Rong‐Ping 
Published: February 2020

AbstractBackground and aimsThe optimal treatment strategy for recurrent hepatocellular carcinoma (HCC) remains unclear. Therefore, we aimed to compare the outcomes of repeat hepatic resection (RHR) and radiofrequency ablation (RFA) for recurrent HCC.MethodFrom December 2004 to December 2015, 138 patients who underwent RHR and 194 patients who underwent RFA were enrolled. Propensity score matching (PSM) was performed to establish 1:1 RHR‐RFA group matching. Clinical outcomes were compared before and after matching.ResultsBefore matching, the 1‐, 3‐, and 5‐year postrecurrence survival (PRS) rates were 91.8%, 82.0%, and 72.9% for the RHR group (n = 138) and 94.4%, 75.4%, and 61.7% for the RFA group (n = 194), respectively (P = .380). After matching, the PRS rates at 1, 3, and 5 years were 90.5%, 81.5%, and 71.8% for the RHR group (n = 120) and 91.0%, 61.0%, and 41.7% for the RFA group (n = 120), respectively (P = .002). In the subgroup analysis, the PRS rates for the RHR group were better than those for the RFA group for patients who relapsed within 2 years (P = .004) or patients with primary tumor burden beyond the Milan criteria (P = .004). Multivariate analysis showed that treatment allocation was identified as an independent prognostic factor for PRS.ConclusionCompared with RFA, RHR provided a survival advantage for recurrent HCC, especially for patients who relapsed within 2 years and those with primary tumor burden beyond the Milan criteria.
Polysplenia syndrome with complex heart disease and jejunal atresia with malrotation in neonate: A case report
Authors: Huseynova Roya Arif , Bin Mahmoud Latifa A. , Abdelrahim Adli , Alroiedy Majeed A. , Huseynov Ogtay 
Published: February 2020

AbstractPolysplenia is heterotaxy syndrome or bilateral left‐sidedness. We report a case of polysplenia syndrome in order to draw attention to this rare syndrome that must be excluded in an infant presenting with congenital heart disease and intestinal malformations.
Agent‐based modeling approach for group polarization behavior considering conformity and network relationship strength
Authors: Zhang Yizhou , Wang Yibao , Chen Tinggui , Shi Jiawen 
Published: February 2020

SummaryCurrently, group behaviors happen frequently with the development of network technology. As a typical social group behavior, group polarization has been attracted more and more academic attention due to its significant disturbance to public's daily lives. At present, the classic J‐A (proposed by Jager and Amblard) and D‐W (proposed by Deffuant and Weisbuch) models are used to analyze group polarization process. However, the main shortcomings existing in these models are that the individuals' psychology and their network relationships are rarely considered. In order to overcome the limitations, this article integrates the influence factors such as conformity and network relationship strength integrated into the polarization model. Besides, the BA (proposed by Barabasi and Albert) network model is used as the agent adjacency model due to its closer to the real social network structure. Subsequently, the experimental simulations are carried out with the multi‐agent Monte‐Carlo method so as to testify the efficiency and effectiveness. The results indicate that different information interaction modes have essential influence on group attitude polarization. Moreover, conformity parameters and the intensity of relationship have dual impacts on both speeding up and slowing down the polarization process.
A parallel generator of non‐Hermitian matrices computed from given spectra
Authors: Wu Xinzhe , Petiton Serge G. , Lu Yutong 
Published: February 2020

SummaryIterative linear algebra methods to solve linear systems and eigenvalue problems with non‐Hermitian matrices are important for both the simulation arising from diverse scientific fields and the applications related to big data, machine learning, and artificial intelligence. The spectral property of these matrices has impacts on the convergence of these solvers. Moreover, with the increase of the size of applications, iterative methods are implemented in parallel on clusters. Analysis of their behaviors with non‐Hermitian matrices on supercomputers is so complex that we need to generate large‐scale matrices with different given spectra for benchmarking. These test matrices should be non‐Hermitian and nontrivial, with high dimension. This paper highlights a scalable matrix generator that constructs large sparse matrices using the user‐defined spectrum, and the eigenvalues of generated matrices are ensured to be the same as the predefined spectrum. This generator is implemented on CPUs and multi‐GPUs platforms, with good strong and weak scaling performance on several supercomputers. We also propose a method to verify its ability to guarantee the given spectra. Finally, we give an example to evaluate the numerical properties and parallel performance of iterative methods using this matrix generator.
Modeling Epithelial Homeostasis and Reactive Epithelial Changes in Human and Murine Three‐Dimensional Esophageal Organoids
Authors: Nakagawa Hiroshi , Kasagi Yuta , Karakasheva Tatiana A. , Hara Takeo , Aaron Bailey , Shimonosono Masataka , Kijima Takashi , Giroux Veronique , Bailey Dominique , Wilkins Benjamin , Abrams Julian A. , Falk Gary W. , Aceves Seema S. , Spergel Jonathan M. , Hamilton Kathryn E. , Whelan Kelly A. , Muir Amanda B. 
Published: February 2020

AbstractThe homeostatic proliferation‐differentiation gradient in the esophageal epithelium is perturbed under inflammatory disease conditions such as gastroesophageal reflux disease and eosinophilic esophagitis. Herein we describe the protocols for rapid generation (<14 days) and characterization of single‐cell‐derived, three‐dimensional (3D) esophageal organoids from human subjects and mice with normal esophageal mucosa or inflammatory disease conditions. While 3D organoids recapitulate normal epithelial renewal, proliferation, and differentiation, non‐cell autonomous reactive epithelial changes under inflammatory conditions are evaluated in the absence of the inflammatory milieu. Reactive epithelial changes are reconstituted upon exposure to exogenous recombinant cytokines. These changes are modulated pharmacologically or genetically ex vivo. Molecular, structural, and functional changes are characterized by morphology, flow cytometry, biochemistry, and gene expression analyses. Esophageal 3D organoids can be translated for the development of personalized medicine in assessment of individual cytokine sensitivity and molecularly targeted therapeutics in esophagitis patients © 2020 by John Wiley & Sons, Inc.Basic Protocol 1: Generation of esophageal organoids from biopsy or murine esophageal epithelial sheetsBasic Protocol 2: Propagation and cryopreservation of esophageal organoidsBasic Protocol 3: Harvesting of esophageal organoids for RNA isolation, immunohistochemistry, and evaluation of 3D architectureBasic Protocol 4: Modeling of reactive epithelium in esophageal organoids.
Rhizoma peanut genotype and planting date affect biomass allocation patterns and establishment performance
Authors: Aryal Parmeshwor , Sollenberger Lynn E. , Kohmann Marta M. , da Silva Liliane S. , Shepard Erin M. , Cooley Katie D. , Rowland Diane L. , Dubeux Jose C. B. 
Published: February 2020

AbstractRhizoma peanut (RP, Arachis glabrata Benth.) is an important perennial forage legume, but its adoption can be limited by relatively slow establishment. Genotype and temperature after planting of rhizomes likely affect partitioning of stored and new photoassimilates, but their impact on establishment rate is not known. To assess these relationships, planting date and RP entry effects were measured on biomass partitioning and growth responses. Treatments were all combinations of two planting dates (spring and summer) and four entries (‘Florigraze’, ‘UF Peace’, ‘UF Tito’, and the germplasm Ecoturf) replicated four times, with new plots planted in each of 2 yr. Spring‐planted Florigraze outperformed UF Peace in shoot count, shoot biomass, and root‐rhizome biomass through much of the year of planting. When planted in summer, UF Tito and UF Peace generally had earlier and greater shoot emergence, lesser root‐rhizome/shoot ratio, taller canopy height, and lesser leaf/stem ratio than Ecoturf or Florigraze. Across years, shoot biomass in the year after planting was greater for UF Peace and UF Tito than for Ecoturf and Florigraze when planted in summer (490, 387, 265, and 157 g m−2, respectively) and greater for UF Peace than Ecoturf or Florigraze planted in spring (349, 243, and 201 g m−2, respectively). UF Tito and UF Peace had greater shoot biomass in the year after planting for summer than for spring plantings, but there was no season effect for Ecoturf and Florigraze. Summer planting favored rapid establishment of upright cultivars UF Tito and UF Peace, but Ecoturf and Florigraze were less affected by season.