bluuebunny/crossref_metadata_2025_split huggingface dataset:
The effect of various concentration of quail egg yolk on spermatozoa motility of kancra fish (Tor soro Valenciennes, 1842) post cryopreservation
Authors: Laeni M , Abinawanto  , Subagja J , Kristanto A H 
Published: February 2020

Abstract
Kancra fish, also known as Batak fish, is often used in North Sumatera in traditional ceremonies. Nevertheless, overfishing has led to a decrease in its population. Cryopreservation is a strategy that might be effectively used to solve this problem. This study aimed to evaluate the effect of various concentrations of quail egg yolk on the spermatozoa motility of kancra fish after 48-hours of cryopreservation. The concentration of quail egg yolk were 0%, 5%, 10%, 15%, 20%, and 25%. The sperm of kancra fish were collected using the stripping method and was diluted with the quail egg yolk, fish ringer, and 10% methanol. It was then equilibrated for 10 minutes at a temperature of 5° C before being frozen at -10 °C for 48 hours. Thawing was carried out at 40 °C for 1 minute. The motility was analyzed using ANOVA and proceeded by Tukey test. The ANOVA test showed the use of various concentrations of quail egg yolk has a significant effect (p<0.05) on the spermatozoa motility of kancra fish after cryopreservation. The concentration of 10% of quail egg yolk showed the highest spermatozoa motility (85.10 ± 1.51%) after 48-hours of cryopreservation (p<0.05).
The spermatozoa motility of kancra fish (Tor soro Valenciennes, 1842) after the frozen process: the application of egg yolk as a cryoprotectant
Authors: Vardini N , Abinawanto  , Subagja J , Kristanto A H 
Published: February 2020

Abstract
Kancra is one of the endemic fishes in Indonesia, however, its population keeps decreasing due to overexploitation. One of the ways methods to solve this problem is by implementing cryopreservation. Cryoprotectant has effect on successful cryopreservation. The purpose of this study was to evaluate the effect of free-range chicken egg yolk as a natural cryoprotectant combined with 10% of methanol to motility of spermatozoa that had been used after 48 hours cryopreservation. The egg yolk concentration used were 0%, 5%, 10%, 15%, 20%, and 25%. The sperm was collected by stripping and diluted with methanol and fish ringer, then it was equilibrated at 5 °C for 10 minutes. After that, it was frozen at -10°C for 48 hours and thawed at 40 °C for 1 minute. The spermatozoa motility was analyze using ANOVA and Tukey test. The results show there is a significant effect (p <0.05) on all of egg yolk concentrations. The 5% concentration of free-range chicken egg yolk shows the highest motility percentage of 84.06 ± 1.67%.
Sperm motility of kancra fish (Tor soro, Valenciennes 1842) after frozen: the effect of soybean milk as a natural cryoprotectant
Authors: Fatriani R , Abinawanto  , Arifin O Z , Kristanto A H 
Published: February 2020

Abstract
Sperm cryopreservation is a way of managing and maintaining kancra fish (Tor soro) resources that increasingly threatened because of over-exploitation. The optimization of sperm cryopreservation is carried out using a natural cryoprotectant with lower toxicity than a chemical cryoprotectant. The purpose of this study was to analyze the effect of soybean milk as a natural cryoprotectant on sperm motility percentage of kancra fish after 48 hours preservation. The sperm was collected through stripping and was diluted into the soluble liquid of fish ringer + methanol 10% + soybean milk. Importantly, the concentration of soybean used were 0%, 5%, 10%, 15%, 20%, and 25%. The sample was equilibrated at the temperature of 5 °C for 10 minutes, frozen at the -10 °C for 48 hours and thawed at 40 °C for 1 minute. The motility percentage was analyzed using ANOVA and Tukey test. The result showed soybean milk had effects (p < 0.05) on the post-cryopreservation sperm motility. Additionally, 5% of soybean milk showed the highest sperm motility (84.37 ± 1.54%).
Prevalence and intensity of protozoan ectoparasite infestation on nursery of humpback grouper (Cromileptes altivelis) in hatchery and floating net cage
Authors: Mahasri G , Subekti S , Angghara B B , Pratama F P 
Published: February 2020

Abstract
Ectoparasites which often attack humpback groupers are protozoa from the Ciliate class, Trichodina sp. and Cryptocaryon sp. This study studied the differences in the prevalence and intensity of protozoan ectoparasites in nursery of humpback grouper in hatcheries and floating net cages. The results showed that there was a significant difference (p <0.05) between the prevalence and intensity of protozoan ectoparasites in nursery of grouper which were kept in hatchery and floating net cages. The prevalence nursery of humpback grouper infested from hatcheries and floating net cages is Trichodina sp. ectoparasites are 83.3% and 76.6%. Prevalence of Cryptocaryon sp. ectoparasites are 76.6% in hatchery and the prevalence of floating net cages is 50%. From the statistical test shows that the intensity of the Trichodina sp. ectoparasites at the hatchery is 12.76 individuals/head was significantly different (p <0.05) on the intensity of the Trichodina sp. ectoparasites. In floating net cages of 4.2 individuals/head. Meanwhile, the results of statistical tests on the intensity of ectoparasite Cryptocaryon sp. at the hatchery is 4.47 individuals/head significantly different (p <0.05) on the intensity of ectoparasites Cryptocaryon sp. in floating net cages there are 1.73 individuals/head.
Growth of Cantang Hybrid Grouper Juvenile (Epinephelus fuscoguttatus x Epinephelus lanceolatus) With Different Feeding Frequency
Authors: Nuraini D , Agustono  , Lutfiyah L 
Published: February 2020

Abstract
Grouper is one of the fish that is much sought after by the general public because grouper have high economic value and one of the exported fish products. The high demand for grouper to cause community develop hatcheries grouper. Appropriate feeding and efficiency very influential towards the process of optimal fish growth. One of the feeding management of frequently used IE frequency feeding. Feed frequency is influenced by several factors, namely age, type and the appetite of the fish itself. The purpose of this study is to see the number of frequency of feeding on seeds of hybrid grouper cantang best to promote growth and feed efficiency and to lower the value of the feed conversion. This study used a RCD. The results showed that the granting of different frequency-feed can increase the growth of specific daily on the seed of hybrid cantang grouper. The highest growth rate of treatment weight i.e. P2 of 4.2 %/day. The value of the efficiency of feed conversion ratio and best i.e. P1 of 67% and 1.55. Treatment of the P1 value is the SGR. efficiency of feed conversion ratio and the lowest i.e. respectively 3.3%/day. 46% and 2.26.
The harmful effect of commercial powder detergent on water flea (Daphnia sp.)
Authors: Kenconojati H , Suciyono  , Azhar M H 
Published: February 2020

Abstract
Detergents are a chemical product that is commonly used in personal care and household product on a daily basis. Its huge consumption in human activities resulted in the increasing awareness of its toxicity in the aquatic environment due to their active ingredients. Daphnia is a planktonic invertebrate organism that usually found in freshwater ecosystems. It plays an essential part in the lower trophic level of food chains. Because of its sensitivity to various environmental changes, they are generally used as a model organism in toxicology. In the present study, we analyzed the toxic effect of household detergent exposure on Daphnia sp. Four different concentration of detergent (25, 50, 75, and 100 mg.L−1) were prepared and the aquadest was used as control. The mortality of Daphnia was observed for 24 hours. The result showed that the mortality of Daphnia increased along with the increasing concentration of detergent exposures. LC50-24 hours values obtained in this study was 28.89 mg.L−1. This result described that the higher level of detergent in a water body can be harmful to the aquatic organism, especially on Daphnia sp. as predominant food for planktivorous fish.
Addition different algae (Spirulina) flour to artificial feed on color quality and growth of Koi fish (Cyprinus carpio-Koi)
Authors: Sudirman D A , Arief M , Fasya A H 
Published: February 2020

Abstrak
Ornamental fish is one of the fisheries commodities that can be used as a source of foreign exchange income, and has its own beauty to attract the interest of ornamental fish lovers. One of the things that determines beauty in many species of ornamental fish is color. Color is a parameter to determine the value of ornamental fish, which is a brighter color, the higher the value of fish. Spirulina sp. Flour has a high carotenoid and protein content, each of which plays a role in increasing color intensity and growth. The purpose of this study was to determine the effect of concentration Spirulina flour to the color quality and growth of koi fish (Cyprinus carpio). The method used was experimental with Completely Randomized Design (CRD) using 4 treatments 5 replications. The dose of feed used is (Control), (dose 1%), (dose 3%) and (dose 5%), Color quality measurement using Adobe Photoshop CS8 software. The frequency of twice-daily feeding for each treatment resulted in color quality with the best value (3.0200%), absolute length growth (0.9780 cm) and absolute weight growth (3.7240 grams).
Tuna fisheries in fisheries management area Republic of Indonesia 572
Authors: Suyasa N , Rahardjo P , Putri D R , Widagdo A 
Published: February 2020

Abstract
This research is expected to improve the sustainable tuna fisheries management of Fisheries Management Area Republic of Indonesia (FMARI) 572 by considering fish resources and fishermen welfare. The objectives of the research are examining the status of tuna fisheries in the FMARI 572, analyzing the bioeconomic model of tuna fisheries in the FMARI 572, and determining the strategy of a sustainable tuna fisheries management in the FMARI 572. This research was carried out for 3 months from February to May 2019 at the existing fishing ports located along the west coast of Sumatra (FMARI 572), such as Lampulo Fishing Port (Aceh), Sibolga Fishing Port (North Sumatra), Bungus Fishing Port (West Sumatra), and Nizam Zachman Fishing Port (Jakarta). Some factors analyzed were: The fishing capacity of fishing units with a variety of fishing gears, the potential of fish resources, the optimum level of efforts reaching the sustainable maximum catches, and the implementation of fisheries bioeconomic models. The results of this study showed the level of utilization tuna in FMARI 572 is still moderate, Tuna fisheries in FMARI 572 have not experienced biological and economical overfishing, and there were 3 sustainable tuna fisheries management strategies proposed.
The relation between quality of the sediment (nitrate, phosphate) and Avicennia sp density, case study; Mangrove Center Bengkak, Banyuwangi Regency, East Java
Authors: Suciyono  , Rahardja B S , Prayoga A I , Kenconojati H , Ulkhaq M F 
Published: February 2020

Abstract
Bengkak mangrove center as a natural environment has been managed by the society as a means of tourism and preservation. These areas were located on Bali strait, consist of fisheries activity and residential. This study aims to determine the density of Avicennia sp, nitrate and phosphate content and the relationship between both. Three stations were determined using purposive sampling and the sample was obtained using the belt transect technique. Linear regression analysis was carried out to determine the density of Avicennia sp, with nitrate, phosphate content. Results of this study showed that the highest density of Avicennia sp was found in station 3 with a value of 3.25, followed by station 2 of 1.38, and the lowest density at station 1 was 0.71. Nevertheless, the highest concentrations of nitrate and phosphate were found at station 3 of 207.27 mg/kg and 0.23%. The relationship between the density of Avicennia sp, with nitrate and phosphate showed a positive correlation.
Screening of antivibrio-producing lactic acid bacteria originated from aquatic animals as probiotic candidates
Authors: Amin M , Liliyanti M A , Nufus N H , Ali M 
Published: February 2020

Abstract
Acute hepatopancreatic necrosis disease has been a major challenge faced by white shrimp farmers in many shrimp-producing countries inducing Indonesia. The etiological agent of this disease was a virulent strain of Vibrio parahaemolyticus. However, a technology to treat or to prevent the disease infection has not been established yet, and probiont could be a potential approach for this disease. Thus, this study aimed at screening 35 lactic acid bacteria (LAB) that originated from the gastrointestinal tract of wild white shrimp for the production of antivibrio compounds using an agar well diffusion. The result showed that three LAB strains (LAB-1, LAB-2, and LAB-3) showed antivibrio activity indicated by the formation of clearance zone (diameter of >10mm) on the diffusion agar plate. When the extracellular products (ECP) were incubated with proteinase K, the antivibrio activity was disappeared which indicated that the antimicrobial compounds were bacteriocin-like inhibitory substances (BLIS). Phenotypic studies suggest that the LAB were Labctobacillus sp. (LAB-1 and LAB-2), and Enterococcus sp. (LB-3). These results suggest these LAB are potential candidates for preventing V. parahaemolyticus. Infection, although, further assays by in vivo using live white shrimp, are still required.
The spatial pattern of turbidity and light transmission around the coastal waters of Cisadane in July and November 2014
Authors: Azis Ismail M F , Prayitno H B 
Published: February 2020

Abstract
We observed the spatial pattern of physical oceanographic parameters, namely seawater turbidity and light transmission around the coastal waters of Cisadane. The observations were taken in July and November 2014 at 18 stations distributed in surrounding coastal waters of Cisadane. Water turbidity and light transmission were measured using CTD SBE 19 censor that could record simultaneously vertical profile of water turbidity and light transmission. The station’s position was determined using a GPS instrument. The results showed that seawater turbidity and light transmission exhibit two distinct spatial and temporal variation which is closely associated with the monsoonal wind system in the region.
Structure communities of macrozoobenthos in mangrove tourism area, Wongsorejo sub-district, Banyuwangi regency, East Java
Authors: Fatmawati D , Rahardja B S , Suciyono  , Lutfiah L , Ulkhaq M F 
Published: February 2020

Abstract
Macrozoobenthos is an organism that lives on the surface or in liquid sediment. Macrozoobenthos is one of the most important organisms for marine and coastal ecosystems. This study aimed to determine the value of the community structure of macrozoobenthos existing in Mangrove Center Bengkak, a subdistrict of Wongsorejo, Banyuwangi regency. Sampling was conducted on three stations with different location characteristics. The community structure was measured by Shannon’s diversity index, Equitability index, and dominance index. The results of this study obtained Shannon’s diversity index of 0.51, equitability index of 0.12, and dominance index of 0.76 at Station 1. At station 2, Shannon’s diversity index of 1.19, equitability index of 0.39, and dominance index of 0.36. While at station 3, it was obtained that Shannon’s diversity index of 1.64, equitability index of 0.85, and dominance index of 0.31.
Social network analysis: Key actors of cooperation between Sama Bajo and land-dwellers in Wakatobi marine national park
Authors: La Ola T , Wianti N I , Tadjuddah M , Suriana  , Buana T , Rosmawaty  , Abdullah S , Wunawarsih I A 
Published: February 2020

Abstract
The relationship between Sama Bajo’s small-scale fishers and land-dwellers are unique and paradox. These ties embedded in the everyday economic and social life of Sama Bajo as an Indonesian iconic maritime tribe. This article will identify key actors between Sama Bajo and varied land-dwellers. We presume that the actors support cooperation and enhance the social resilience of Sama Bajo communities in marine preserve social context. The research was conducted from May until June 2019 in three Sama Bajo villages and land-dwellers islands in Wakatobi Regency. A social mapping, using UCINET 6.0, is offered to seek key actors in the different local social context of land-dwellers. Research findings found that key actors related to trading networks, social identity, and gender dimension. Later, the actors’ social relation has contributed to the social resilience amongst three different Sama Bajo communities in Wakatobi Marine National Park.
Addition of lemuru fish oil in the diet on the fat retention and fatty acid profile of silver barb (Rasbora argyrotaenia)
Authors: Marini A , Mubarak A S , Mukti A T 
Published: February 2020

AbstractOne important factor in cultivation is feed. The addition of fish oil to feed affects the activity of the protein and cell synthesis so that it affects the growth rate, feed conversion ratio, and protein and fat contents of the fish body. This study aimed to determine the effect of the addition of lemuru fish oil in feed on fat retention and fatty acid profiles of silver barb (Rasbora argyrotaenia) fish. This study was used as an experimental method with a completely randomized design consisting of five treatments and four replications for each treatment. The lemuru fish oil with different concentrations, namely 0, 2, 4, 6, and 8% were added to commercial feed of silver barb. The parameters of fat retention and fatty acid profile were observed. Data were analyzed statistically using a variant of analysis followed by Duncan’s multiple range test. The results showed that the addition of lemuru fish oil in the fish feed for 40 days of fish rearing had a significant effect (p<0.05) on fat retention and increased fatty acid profile of silver barb. The 2% treatment has the best of fat retention (53.36%). A fat concentration of 9% in the diet increase unsaturated fatty acids content of silver barb (66.32%).
Addition of Lemuru Fish Oil to Protein Retention and Feed Utilization Efficiency of silver barb Rasbora argyrotaenia
Authors: Ayunda R , Sulmartiwi L , Mubarak A S 
Published: February 2020

Abstract
Silver barb Rasbora argyrotaenia is one of the local freshwater fish in indonesia that has advantage of being a source of protein and also resistant to disease. This study aims to determine the effect of adding lemuru fish oil to feeds on protein retention and the feed utilization efficiency of silver barb. This study used an experimental method with a complete randomized design consisting of five treatments and four replications. The treatment given is commercial food which is added with lemuru fish oil with different concentrations. In this study it can be seen that the treatments tested were: P0 (0%), P1 (2%), P2 (4%), P3 (6%), P4 (8%). The parameters observed in this study were protein retention and feed utilization efficiency of silver barb. Data analysis using Variant Analysis (ANOVA) and continued with Duncan’s Multiple Distance Test. The results showed that the addition of lemuru fish oil in feed had a significant effect (P<0,05) on protein retention and feed utilization efficiency of silver barb. The highest protein retention in treatment P2 (4%) of 16.95% and the highest efficiency of feed utilization was also found in the treatment of P2 (4%) of 57.93%.
Study on mangrove canopy cover in Lembeh Island, North Sulawesi
Authors: Rumengan A P , Paruntu C P , Paransa D , Rumengan S H 
Published: February 2020

Abstract
The purpose of this study is to monitor the cover of mangrove forests on Lembeh Island, North Sulawesi, to support various protections. The method used to determine mangrove health uses the value of Normalized Different Vegetation Index (NDVI). NDVI value of mangrove forest cover in 2016 and 2019 using Landsat image 8. Mangrove health in Lembeh Island Seen more in 2016 and 2019. Percentage of mangrove forest cover based on Minister of Environment Decree No. 201 of 2004 concerning standard criteria and guidelines for determining mangrove health. The rate obtained based on the dense category increased from 65% in 2016 to 67% in 2019, while mangrove in the group spares decreased from 13% percent in 2016 to 10% in 2019.
The immune responses of Oreochromis niloticus under different form of Bacillus supplementation
Authors: Yuniarti A , Arifin N B , Muawiyah N , Fakhri M , Hariati A M 
Published: February 2020

Abstract
Probiotic is considered an effective means for disease prevention in the aquaculture system. The most of probiotic species used in aquaculture were Bacillus in the form of vegetative cells. Therefore, this study evaluates the immune responses of Oreochromis niloticus under the Bacillus supplementation in the form of vegetative cells and spore. The addition of vegetative cells and spore Bacillus was given in the fish diet for 49 days. Several non-specific immune responses were evaluated afterward. Treatment without Bacillus supplementation in the diet was used as a control. The results showed that Bacillus supplementation in the form of spore and vegetative increased the non-specific immune response compared to those of control. Some parameters of the immune response, such as total leukocytes, haematocrit value, and respiratory burst, were affected by the form of Bacillus supplementation. Total haematocrit (31.67%), total leukocytes (8,2x104 cells.mm-3), Lymphocyte (81.33%) and respiratory burst (0.09 nm) of Tilapia with spore Bacillus supplementation were noted as the highest value. On the other hand, the phagocytosis activity of Tilapia was found statistically similar to vegetative or spore form of Bacillus supplementation. The factor that affected those results was the higher viability of the Bacillus spore in the fish diet. This study indicated that Bacillus supplementation in the form of spore gave the best improvement on the Tilapia non-specific immune response and could maintain the health status of the fish.
Growth rate and survivorship of Acropora sp. fragments that transplanted on the artificial substrate made from fly ash and bottom ash
Authors: Khasanah R I , Herawati E Y , Hariati A M , Mahmudi M , Sartimbul A , Wiadnya D G R , Asrial E , Yudatomo  , Nabil E 
Published: February 2020

Abstract
The purpose of this research is to analyze the success the transplantation method using kerbstone media from blocks made of coal waste (fly ash and bottom ash) from Thermal Power Station (PLTU). The research was done from March to August 2018 in the waters of Paiton, Probolinggo, East Java, Indonesia. The method applied in this research a 7-block design with “H” formation for every module. The coral fragments of Acropora formosa, Acropora pulchra, Acropora intermedia, and Acropora gomezi was 10 cm in average size with 30 repetitions for each size. The average growth rate showed different values during five months of research since the transplantation was done. Acropora formosa had the highest growth rate of 1.958 cm/month. Acropora intermedia 1.730 cm/month, Acropora pulchra 0.958 cm/month, and 0.756 cm/month for Acropora gomezi. By using Kruskal Wallis test for data analysing, obtained a survival rate of over 80% for all types, with Acropora formosa as the highest (92.3%) and Acropora gomezi as the lowest (82.61%). Therefore, kerbstone is considered feasible as a growth media for the coral reef as an effort to support CTI (Coral Triangle Initiative) program in overcoming global warming as an innovation.