bluuebunny/crossref_metadata_2025_split huggingface dataset:
RETRACTED ARTICLE: A newly isolated strain of Halomonas sp. (HA1) exerts anticancer potential via induction of apoptosis and G2/M arrest in hepatocellular carcinoma (HepG2) cell line
Authors: El-Garawani Islam M. , El-Sabbagh Sabha M. , Abbas Nasser H. , Ahmed Hany S. , Eissa Omaima A. , Abo-Atya Doaa M. , Khalifa Shaden A. M. , El-Seedi Hesham R. 
Published: August 2020

AbstractMarine bacterial strains are of great interest for their ability to produce secondary metabolites with anticancer potentials. Isolation, identification, characterization and anticancer activities of isolated bacteria from El-Hamra Lake, Wadi El-Natrun (Egypt) were the objectives of this study. The isolated bacteria were identified as a moderately halophilic alkaliphilic strain. Ethyl acetate extraction was performed and identified by liquid chromatography-mass spectrophotometry (LC–MS–MS) and nuclear magnetic resonance analysis (NMR). Cytotoxicity of the extract was assessed on the HepG2 cell line and normal human peripheral lymphocytes (HPBL) in vitro. Halomonas sp. HA1 extract analyses revealed anticancer potential. Many compounds have been identified including cyclo-(Leu-Leu), cyclo-(Pro-Phe), C17-sphinganine, hexanedioic acid, bis (2-ethylhexyl) ester, surfactin C14 and C15. The extract exhibited an IC50 of 68 ± 1.8 μg/mL and caused marked morphological changes in treated HepG2 cells. For mechanistic anticancer evaluation, 20 and 40 µg/mL of bacterial extract were examined. The up-regulation of apoptosis-related genes' expression, P53, CASP-3, and BAX/BCL-2 at mRNA and protein levels proved the involvement of P53-dependant mitochondrial apoptotic pathway. The anti-proliferative properties were confirmed by significant G2/M cell cycle arrest and PCNA down-regulation in the treated cells. Low cytotoxicity was observed in HPBL compared to HepG2 cells. In conclusion, results suggest that the apoptotic and anti-proliferative effects of Halomonas sp. HA1 extract on HepG2 cells can provide it as a candidate for future pharmaceutical industries.
The D2.mdx mouse as a preclinical model of the skeletal muscle pathology associated with Duchenne muscular dystrophy
Authors: Hammers David W. , Hart Cora C. , Matheny Michael K. , Wright Lillian A. , Armellini Megan , Barton Elisabeth R. , Sweeney H. Lee 
Published: August 2020

AbstractDuchenne muscular dystrophy (DMD) is an X-linked, lethal muscle degenerative disease caused by loss of dystrophin protein. DMD has no cure and few treatment options. Preclinical efforts to identify potential DMD therapeutics have been hampered by lack of a small animal model that recapitulates key features of the human disease. While the dystrophin-deficient mdx mouse on the C57BL/10 genetic background (B10.mdx) is mildly affected, a more severe muscle disease is observed when the mdx mutation is crossed onto the DBA/2J genetic background (D2.mdx). In this study, the functional and histological progression of the D2.mdx skeletal muscle pathology was evaluated to determine the distinguishing features of disease. Data herein details the muscular weakness and wasting exhibited by D2.mdx skeletal muscle, as well as severe histopathological features, which include the rapid progression of fibrosis and calcifications in the diaphragm and progressive fibrosis accumulation in limb muscles. Furthermore, a timeline of D2.mdx progression is provided that details distinct stages of disease progression. These data support the D2.mdx as a superior small animal model for DMD, as compared to the B10.mdx model. The insights provided in this report should facilitate the design of preclinical evaluations for potential DMD therapeutics.
TGF-β is insufficient to induce adipocyte state loss without concurrent PPARγ downregulation
Authors: Taylor Brooks , Shah Arnav , Bielczyk-Maczyńska Ewa 
Published: August 2020

AbstractCell plasticity, the ability of differentiated cells to convert into other cell types, underlies the pathogenesis of many diseases including the transdifferentiation of adipocytes (fat cells) into myofibroblasts in the pathogenesis of dermal fibrosis. Loss of adipocyte identity is an early step in different types of adipocyte plasticity. In this study, we determine the dynamics of adipocyte state loss in response to the profibrotic cytokine TGF-β. We use two complementary approaches, lineage tracing and live fluorescent microscopy, which both allow for robust quantitative tracking of adipocyte identity loss at the single-cell level. We find that the intracellular TGF-β signaling in adipocytes is inhibited by the transcriptional factor PPARγ, specifically by its ubiquitously expressed isoform PPARγ1. However, TGF-β can lead to adipocyte state loss when it is present simultaneously with another stimulus. Our findings establish that an integration of stimuli occurring in a specific order is pivotal for adipocyte state loss which underlies adipocyte plasticity. Our results also suggest the possibility of a more general switch-like mechanism between adipogenic and profibrotic molecular states.
The oligomeric assembly of galectin-11 is critical for anti-parasitic activity in sheep (Ovis aries)
Authors: Sakthivel Dhanasekaran , Preston Sarah , Gasser Robin B. , Costa Tatiana P. Soares da , Hernandez Julia N. , Shahine Adam , Shakif-Azam M. D. , Lock Peter , Rossjohn Jamie , Perugini Matthew A. , González Jorge Francisco , Meeusen Els , Piedrafita David , Beddoe Travis 
Published: August 2020

AbstractGalectins are a family of glycan-binding molecules with a characteristic affinity for ß-D-glycosides that mediate a variety of important cellular functions, including immune and inflammatory responses. Galectin-11 (LGALS-11) has been recently identified as a mediator induced specifically in animals against gastrointestinal nematodes and can interfere with parasite growth and development. Here, we report that at least two natural genetic variants of LGALS-11 exist in sheep, and demonstrate fundamental differences in anti-parasitic activity, correlated with their ability to dimerise. This study improves our understanding of the role of galectins in the host immune and inflammatory responses against parasitic nematodes and provides a basis for genetic studies toward selective breeding of animals for resistance to parasites.
Proteosomal degradation of NSD2 by BRCA1 promotes leukemia cell differentiation
Authors: Park Jin Woo , Kang Joo-Young , Hahm Ja Young , Kim Hyun Jeong , Seo Sang-Beom 
Published: August 2020

AbstractThe human myelogenous leukemic cell line, K562 undergoes erythroid differentiation by exposure to hemin. Here, we uncovered NSD2 as an innate erythroid differentiation-related factor through a genome-wide CRISPR library screen and explored the regulatory role of NSD2 during myeloid leukemia cell differentiation. We found that NSD2 stability was disrupted by poly-ubiquitination in differentiated K562 cells. Proteomic analysis revealed an interaction between NSD2 and an E3 ubiquitin ligase, BRCA1, which ubiquitylates NSD on K292. Depletion of BRCA1 stabilized NSD2 protein and suppressed K562 cell differentiation. Furthermore, BRCA1 protein level was decreased in bone marrow tumor, while NSD2 level was elevated. Surprisingly, among BRCA1 mutation(s) discovered in lymphoma patients, BRCA1 K1183R prevented its translocation into the nucleus, failed to reduce NSD2 protein levels in hemin-treated K562 cells and eventually disrupted cell differentiation. Our results indicate the regulation of NSD2 stability by BRCA1-mediated ubiquitination as a potential therapeutic target process in multiple myeloma.
Glycovariant-based lateral flow immunoassay to detect ovarian cancer–associated serum CA125
Authors: Bayoumy Sherif , Hyytiä Heidi , Leivo Janne , Talha Sheikh M. , Huhtinen Kaisa , Poutanen Matti , Hynninen Johanna , Perheentupa Antti , Lamminmäki Urpo , Gidwani Kamlesh , Pettersson Kim 
Published: August 2020

AbstractCancer antigen 125 (CA125) is a widely used biomarker in monitoring of epithelial ovarian cancer (EOC). Due to insufficient cancer specificity of CA125, its diagnostic use is severely compromised. Abnormal glycosylation of CA125 is a unique feature of ovarian cancer cells and could improve differential diagnosis of the disease. Here we describe the development of a quantitative lateral flow immunoassay (LFIA) of aberrantly glycosylated CA125 which is widely superior to the conventional CA125 immunoassay (CA125IA). With a 30 min read-out time, the LFIA showed 72% sensitivity, at 98% specificity using diagnostically challenging samples with marginally elevated CA125 (35–200 U/mL), in comparison to 16% sensitivity with the CA125IA. We envision the clinical use of the developed LFIA to be based on the substantially enhanced disease specificity against the many benign conditions confounding the diagnostic evaluation and against other cancers.
Iterative evolution of large-bodied hypercarnivory in canids benefits species but not clades
Authors: Balisi Mairin A. , Van Valkenburgh Blaire 
Published: August 2020

AbstractEcological specialization has costs and benefits at various scales: traits benefitting an individual may disadvantage its population, species or clade. In particular, large body size and hypercarnivory (diet over 70% meat) have evolved repeatedly in mammals; yet large hypercarnivores are thought to be trapped in a macroevolutionary “ratchet”, marching unilaterally toward decline. Here, we weigh the impact of this specialization on extinction risk using the rich fossil record of North American canids (dogs). In two of three canid subfamilies over the past 40 million years, diversification of large-bodied hypercarnivores appears constrained at the clade level, biasing specialized lineages to extinction. However, despite shorter species durations, extinction rates of large hypercarnivores have been mostly similar to those of all other canids. Extinction was size- and carnivory-selective only at the end of the Pleistocene epoch 11,000 years ago, suggesting that large hypercarnivores were not disadvantaged at the species level before anthropogenic influence.
Relative importance of nonlinear electron-phonon coupling and vertex corrections in the Holstein model
Authors: Dee Philip M. , Coulter Jennifer , Kleiner Kevin G. , Johnston Steven 
Published: August 2020

AbstractDetermining the range of validity of Migdal’s approximation for electron-phonon (e-ph) coupled systems is a long-standing problem. Many attempts to answer this question employ the Holstein Hamiltonian, where the electron density couples linearly to local lattice displacements. When these displacements are large, however, nonlinear corrections to the interaction must also be included, which can significantly alter the physical picture obtained from this model. Using determinant quantum Monte Carlo and the self-consistent Migdal approximation, we compared superconducting and charge-density-wave correlations in the Holstein model with and without second-order nonlinear interactions. We find a disagreement between the two cases, even for relatively small values of the e-ph coupling strength, and, importantly, that this can occur in the same parameter regions where Migdal’s approximation holds. Our results demonstrate that questions regarding the validity of Migdal’s approximation go hand in hand with questions of the validity of a linear e-ph interaction.
All-optical supercontinuum switching
Authors: Melchert Oliver , Brée Carsten , Tajalli Ayhan , Pape Alexander , Arkhipov Rostislav , Willms Stephanie , Babushkin Ihar , Skryabin Dmitry , Steinmeyer Günter , Morgner Uwe , Demircan Ayhan 
Published: August 2020

AbstractEfficient all-optical switching is a challenging task as photons are bosons and cannot immediately interact with each other. Consequently, one has to resort to nonlinear optical interactions, with the Kerr gate being the classical example. However, the latter requires strong pulses to switch weaker ones. Numerous approaches have been investigated to overcome the resulting lack of fan-out capability of all-optical switches, most of which relied on types of resonant enhancement of light-matter interaction. Here we experimentally demonstrate a novel approach that utilizes switching between different portions of soliton fission induced supercontinua, exploiting an optical event horizon. This concept enables a high switching efficiency and contrast in a dissipation free setting. Our approach enables fan-out, does not require critical biasing, and is at least partially cascadable. Controlling complex soliton dynamics paves the way towards building all-optical logic gates with advanced functionalities.
Unpuzzling COVID-19: tissue-related signaling pathways associated with SARS-CoV-2 infection and transmission
Authors: Battagello Daniella S. , Dragunas Guilherme , Klein Marianne O. , Ayub Ana L.P. , Velloso Fernando J. , Correa Ricardo G. 
Published: August 2020

Abstract
The highly infective coronavirus disease 19 (COVID-19) is caused by a novel strain of coronaviruses – the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) – discovered in December 2019 in the city of Wuhan (Hubei Province, China). Remarkably, COVID-19 has rapidly spread across all continents and turned into a public health emergency, which was ultimately declared as a pandemic by the World Health Organization (WHO) in early 2020. SARS-CoV-2 presents similar aspects to other members of the coronavirus family, mainly regarding its genome, protein structure and intracellular mechanisms, that may translate into mild (or even asymptomatic) to severe infectious conditions. Although the mechanistic features underlying the COVID-19 progression have not been fully clarified, current evidence have suggested that SARS-CoV-2 may primarily behave as other β-coronavirus members. To better understand the development and transmission of COVID-19, unveiling the signaling pathways that may be impacted by SARS-CoV-2 infection, at the molecular and cellular levels, is of crucial importance. In this review, we present the main aspects related to the origin, classification, etiology and clinical impact of SARS-CoV-2. Specifically, here we describe the potential mechanisms of cellular interaction and signaling pathways, elicited by functional receptors, in major targeted tissues/organs from the respiratory, gastrointestinal (GI), cardiovascular, renal, and nervous systems. Furthermore, the potential involvement of these signaling pathways in evoking the onset and progression of COVID-19 symptoms in these organ systems are presently discussed. A brief description of future perspectives related to potential COVID-19 treatments is also highlighted.
The Gaia-ESO Survey: Calibrating the lithium–age relation with open clusters and associations
Authors: Gutiérrez Albarrán M. L. , Montes D. , Gómez Garrido M. , Tabernero H. M. , González Hernández J. I. , Marfil E. , Frasca A. , Lanzafame A. C. , Klutsch A. , Franciosini E. , Randich S. , Smiljanic R. , Korn A. J. , Gilmore G. , Alfaro E. J. , Baratella M. , Bayo A. , Bensby T. , Bonito R. , Carraro G. , Delgado Mena E. , Feltzing S. , Gonneau A. , Heiter U. , Hourihane A. , Jiménez Esteban F. , Jofre P. , Masseron T. , Monaco L. , Morbidelli L. , Prisinzano L. , Roccatagliata V. , Sousa S. , Van der Swaelmen M. , Worley C. C. , Zaggia S. 
Published: August 2020

Context. Previous studies of open clusters have shown that lithium depletion is not only strongly age dependent but also shows a complex pattern with other parameters that is not yet understood. For pre- and main-sequence late-type stars, these parameters include metallicity, mixing mechanisms, convection structure, rotation, and magnetic activity.
Aims. We perform a thorough membership analysis for a large number of stars observed within the Gaia-ESO survey (GES) in the field of 20 open clusters, ranging in age from young clusters and associations, to intermediate-age and old open clusters.
Methods. Based on the parameters derived from the GES spectroscopic observations, we obtained lists of candidate members for each of the clusters in the sample by deriving radial velocity distributions and studying the position of the kinematic selections in the EW(Li)-versus-Teff plane to obtain lithium members. We used gravity indicators to discard field contaminants and studied [Fe/H] metallicity to further confirm the membership of the candidates. We also made use of studies using recent data from the Gaia DR1 and DR2 releases to assess our member selections.
Results. We identified likely member candidates for the sample of 20 clusters observed in GES (iDR4) with UVES and GIRAFFE, and conducted a comparative study that allowed us to characterize the properties of these members as well as identify field contaminant stars, both lithium-rich giants and non-giant outliers.
Conclusions. This work is the first step towards the calibration of the lithium–age relation and its dependence on other GES parameters. During this project we aim to use this relation to infer the ages of GES field stars, and identify their potential membership to young associations and stellar kinematic groups of different ages.
A charging model for the Rosetta spacecraft
Authors: Johansson F. L. , Eriksson A. I. , Gilet N. , Henri P. , Wattieaux G. , Taylor M. G. G. T. , Imhof C. , Cipriani F. 
Published: August 2020

Context.The electrostatic potential of a spacecraft,VS, is important for the capabilities of in situ plasma measurements. Rosetta has been found to be negatively charged during most of the comet mission and even more so in denser plasmas.Aims.Our goal is to investigate how the negativeVScorrelates with electron density and temperature and to understand the physics of the observed correlation.Methods.We applied full mission comparative statistics ofVS, electron temperature, and electron density to establishVSdependence on cold and warm plasma density and electron temperature. We also used Spacecraft-Plasma Interaction System (SPIS) simulations and an analytical vacuum model to investigate if positively biased elements covering a fraction of the solar array surface can explain the observed correlations.Results.Here, theVSwas found to depend more on electron density, particularly with regard to the cold part of the electrons, and less on electron temperature than was expected for the high flux of thermal (cometary) ionospheric electrons. This behaviour was reproduced by an analytical model which is consistent with numerical simulations.Conclusions.Rosetta is negatively driven mainly by positively biased elements on the borders of the front side of the solar panels as these can efficiently collect cold plasma electrons. Biased elements distributed elsewhere on the front side of the panels are less efficient at collecting electrons apart from locally produced electrons (photoelectrons). To avoid significant charging, future spacecraft may minimise the area of exposed bias conductors or use a positive ground power system.
Die „Handballer-Schulter“ im Fokus von Diagnostik und Therapie
Authors: Hepp Pierre , Henkelmann Ralf 
Published: August 2020

ZusammenfassungHandball zählt zu den beliebtesten Mannschaftssportarten in Deutschland. Bei Diagnostik und Therapie von Schulterverletzungen und Schulterbeschwerden muss zwischen der sportartspezifischen jahrelangen Adaptation und einer vermeintlichen Dekompensation zwingend unterschieden werden. Im Gegensatz zu reinen Wurfsportarten, wie z. B. Baseball oder Speerwerfen, werden im Handball wegen des gleichzeitigen Gegnerkontaktes die beabsichtigten Bewegungsabläufe durch erlaubte und unerlaubte Behinderungen oft plötzlich und ruckartig beeinflusst. Hierdurch können zusätzliche Verletzungen entstehen. Der Übergang einer physiologisch adaptierten Handballer-Schulter zu einer therapiebedürftigen pathologischen Werfer-Schulter ist meist fließend. Die Werfer-Schulter ist durch eine Leistungseinschränkung charakterisiert, die durch Schmerzen in der Schulter bei wiederholter sportartspezifischer Belastung hervorgerufen wird. Neben einer gründlichen klinischen Untersuchung ist, aufgrund der Tatsache, dass Veränderungen der Handballer-Schulter sich in der Muskultur, den Sehnen und im Knochen im Sinne von Überlastungsreaktionen (bone bruise) darstellen, das MRT die bevorzugte Untersuchungsmethode. Neben einer Sportkarenz und einer suffizienten Schmerztherapie sollte eine gezielte Physiotherapie erfolgen. Die Interpretation der MRT-Ergebnisse sollte immer mit den Symptomen und den klinischen Untersuchungsergebnissen abgeglichen werden. Indikationen zur Operation sind eine ausbleibende Besserung nach gezielter Schmerz- und Physiotherapie, strukturelle Schäden entsprechend der Symptome ohne Heilungschance bzw. mit höherer Gewissheit der Verbesserung der Beschwerden und Funktion durch eine Operation. Nach einer operativen Therapie spielt die anschließende Rehabilitation eine große Rolle. Eine Sportfähigkeit ist je nach Verletzung in bis zu 90 % wieder möglich.
Comparing major joint injuries, interventions and late sequelae in elite male handball players with an age-matched control group
Authors: Vogel Raphael , Zdravkovic Vilijam , Badulescu Michael , Puskás Gábor J. , Jost Bernhard 
Published: August 2020

Abstract
Introduction Handball is a contact sport which involves throwing and jumping, exposing players to serious physical stress. There is a high risk of injuries leading to possible long-term sequelae. The aim of this study was to assess the incidence of musculoskeletal injuries in elite male handball players compared with an age-matched control group.
Patients and methods Former elite handball players, who had played on the Swiss national team between 1980 and 1985, answered a questionnaire about injuries, surgical interventions and their current health status. A total of 34 athletes were compared with 58 age-matched volunteers, who only engaged in recreational sports or no sports at all.
Results The mean age of the athletes was 58.4 years (range 52–68 years) and did not differ significantly from the mean age of the control group of 58.7 years (range 53–69 years). In the control group, 70 % engaged in recreational sports. There was no statistical difference regarding the life-long incidence of shoulder injuries and surgical interventions, sequelae or persistent shoulder pain. Athletes had more interventions after elbow injuries (0.09 vs. 0, p = 0.047), but the difference with respect to chronic pain or late sequelae was not statistically significant. For knee injuries, there were no significant differences regarding the incidence of injuries or interventions, the prevalence of secondary consequences or persistent pain. Concerning the foot and ankle, there was a significantly higher incidence of injuries (0.5 vs. 0.03, p < 0.001) and interventions (0.5 vs. 0.09, p < 0.001) in athletes, but no statistical difference regarding sequelae or persistent pain. Overall quality of life had identical ratings in both groups (athletes mean 85.9 %, controls mean 85.8 %).
Discussion Top handball players did not sustain more shoulder or knee injuries than the age-matched control group. The elbow was more at risk in these top athletes, but long-term consequences appeared to be less severe. The most distinctive difference was seen in foot and ankle injuries.
Conclusions A career as an elite handball player had no adverse effect on the overall quality of life of elite handball players 25 to 30 years after retiring from professional sports.