bluuebunny/crossref_metadata_2025_split huggingface dataset:
Categorization of temocillin susceptibility using the disc diffusion method: Susceptible, Intermediate, Resistant or Undetermined?
Authors: HENRY Amandine , Michaud Anthony , Degand Nicolas , Bille Emmanuelle , Fournier Damien , Capitaine Agathe , Brun Cecile Le , Lourtet-Hascoët Julie , Guillard Thomas , Corvec Stéphane , Plouzeau-Jayle Chloé , Guillotel Elena , Farfour Eric 
Published: January 2020

Abstract
Background: Since the pandemic of ESBL (extended-spectrum beta-lactamases) and the emergence of carbapenemase-producing Enterobacterales there is a renewed interest in temocillin. However, as the molecule was little used, except in Belgium and UK, there are few guidelines for its antibiotic susceptibility testing (AST). We aim to assess the accuracy of the disc diffusion method (DDM) for temocillin susceptibility testing. Methods: Eight hundred eighty-eight Enterobacterales clinical strains of which 61.7% were resistant to 3 rd generation cephalosporin (3GC-R) were included. AST was performed using DDM in comparison to gradient strip tests (GST) and interpreted using the diameter breakpoint of 20mm and MIC breakpoint of 8 mg/L for DDM and GST as recommended by EUCAST-CASFM recommandations. Results: At breakpoint of 8mg/L, temocillin rates of susceptibility were 76.9%, 95.6% and 67.7% for overall, 3GC-S and 3GC-R strains respectively. Ninety-four (10.6%) discrepancies were noticed including 68 (72.3%) major errors and 26 (27.6%) very major errors. Sixty-eight (72.3%) of all errors correspond to inhibition diameters comprised between 17 and 23 mm. The presence of colonies within the inhibition diameter was noticed for 32 (5.3%) strains susceptible to temocillin. Conclusions: DDM lacks accuracy for strains displaying borderline inhibition zone diameter. We suggest applying the concept of area of technical uncertainty (ATU) for standard DDM. The strains displaying a diameter within the ATU should be tested by another method. The significance of colonies within the inhibition zone diameter should be explored.
Root exudate input stimulates peatland recalcitrant DOC decomposition by r-strategic taxa of Gammaproteobacteria and Bacteroidetes
Authors: Mastný Jiří , Bárta Jiří , Kaštovská Eva , Picek Tomáš 
Published: January 2020

Abstract
Background In peatlands, decomposition of organic matter is limited by harsh environmental conditions and low decomposability of the plant material. Increased microbial decomposition of organic matter in peatland ecosystems may become an important phenomenon in the near future after the expected shift in plant community composition from Sphagnum to vascular plants due to climate change. Such a change in plant community composition will lead to increased root exudates flux to the soil and stimulation of microbial growth and activity. The aim of our study was to evaluate the effect of root exudates on the decomposition of recalcitrant dissolved organic carbon (DOC) and identify the microorganisms responsible for this process. Results Decomposition of recalcitrant DOC was stimulated by a high levels of 13 C labelled root exudates addition whereas it was suppressed by a low levels of root exudates addition. Recalcitrant DOC decomposition was positively related to the exudate C/N ratio as a result of enhanced “microbial nutrient mining” due to a deepening of microbial nutrient limitation. The early stage of incubation immediately following the exudate addition was characterized by the preferential use of the added compounds at the expense of recalcitrant DOC. At the same time, r-strategic bacteria (identification based on average 16SrRNA gene copy number) belonging to mainly to Gammaproteobacteria and Bacteriodete s phyla relatively increased within the microbial community. At the later stage, this more abundant bacterial community was replaced by a less abundant community composed of bacteria mostly belonging to Alphaproteobacteria and Acidobacteria . The most important taxa with the potential to decompose complex compounds were indentified: Mucilaginibacter ( Bacteriodete s), Burkholderia and Pseudomonas ( Gammaproteobacteria ) among r-strategists and Bryocella and Candidatus Solibacter ( Acidobacteria ) among K-strategists. Conclusions Increased inputs of root exudates, with a higher C/N ratio, may stimulate decomposition of peatland recalcitrant DOC. In this, bacteria were found to be more important than fungi. Our experiment indicates that r-strategic bacteria as well as K-strategists can be important in stimulated decomposing of recalcitrant peatland DOC.
MALAT1 plays important role in MEK inhibitor, RG7420, on the proliferation and migration of endometrial cancer cell through sponging miR-129-5p/ TAK1
Authors: Shen Yuan , Mengjun Shan , LU Lin , Ding Xiaomin , Sun Yuanjue , Zhang Jianjun , He Aina , Wang Yaling , Zhou Yan , Su Yang , Yin Junyi , Shen Zan , Yao Yang , Li Ruiman , Hu Haiyan 
Published: January 2020

Abstract
Background: Endometrial cancer is one of the most common malignancies of the female genital tract. Although the overall five-year survival of EC is much higher than other genital tumor, 25% of the total patients with high risk to develop more aggressive diseases. The metastasis in EC patients is the main cause of death. The MEK inhibitor, which has significant effect on malignant molenoma, lung cancer and so on, has bright future in EC. Method: Here we treated the EC cell line HEC50 and HEC1A with different concentration of RG7420. The CCK8 was used to detecte the cell proliferation rate. Microarray analyzed the difference of LncRNAs in EC cells with or without RG7420. Luciferase reporter assay and RNA immunoprecipitation assay(RIP) were performed to verified the regulation among MALAT1, miR-129-5p and TGF-β-activated kinase 1 (TAK1). QRT-PCR and WB were used to detect the changes in mRNA and protein levels. We also performed ISH to detect the MALAT level in EC paraffin section. Results: We found the RG7420 significantly inhibited the viability and mobility of EC cell lines. Then we analyzed the profile of LncRNA in HEC50 with or without RG7420. We found after treated with IC50 of HEC50, the metastasis-associated lung adenocarcinoma transcript-1(MALAT1) decreased 6.13 times with up-regulation of tumor suppressor miR-129-5p. Our data also provided that MALAT1 may work as endogenous sponge for miR-129-5p. Here we predicted and proved TGF-β-activated kinase 1 (TAK1), encoded by MAP3K7, is the target of miR-129-5p and works as the key factor in metastasis of EC. Meanwhile we compared the MALAT1 level in EC paraffin section by in-situ hybridization. Rerospective analysis assessed the corelation between the MALAT1 level and the clinical characters of EC, MALAT1 is a poor prognostic marker of EC. Furthermore we over-expressed MALAT1 in EC cells, the function of RG7420 was reversed. Conclusion: Taken together, our data uncovered RG7420 inhibited the proliferation and migration of EC cell through MALAT1/miR-129-5p/TAK1 pathway. MALAT1 is a poor prognostic marker of EC.
Tetraploidy enhances the cold-resistance in yellow kiwifruit (Actinidia chinensis)
Authors: Li Yipei , Liu Xiaozhen , Wei Zhou , Zhang Zhimin , Bian Wen , Li Shengxing , Zhang Hanyao 
Published: January 2020

Abstract
Yellow kiwifruit ( Actinidia chinensis ) is highly susceptible to severe weather, such as low temperature and frost, which may affect the production in the coming year. And the cold-resistant mechanism of kiwifruit associated with gene regulation is poorly investigated. To botain cold-resistant germplam, to provide insight into the causes of differences in low temperature tolerance due to ploidy and to better understand cold-adaptive mechanisms in tetraploid kiwifruit, the diploid yellow kiwifruit ‘SWFU03’ and its tetraploid plantlets were subjected to cold-tolerant screening with L-hydroxyproline (L-Hyp) and low temperature, the selected ones were then analyzed by transcriptome data and confirmed by RT-qPCR. The results showed that the survival rate of tetraploid plants was 62.22% when treated with 8 mmol/L L-Hyp for 30 days, while all the diploid ones died. After treated with 0°C for 12 h, then at room temperature for seven days, the survival rate of tetraploid plantlets was 42.22%, while all diploidy died. Hence, cold tolerance of the tetraploid plantlets was stronger than that of the diploid genotypes. Using these two screening systems, 126 cold-resistant tetraploid tissue culture plantlets were obtained. A total of 1630 differentially expressed genes (DEGs) were identified, of which 619 were up-regulated and 1011 were down-regulated in the low temperature treatment goup. The DEGs enriched in the cold-tolerance related pathways mainly included plant hormone signal transduction, and starch and sucrose metabolism pathway. RT-qPCR analysis confirmed the expression levels of eight up-regulated genes in these pathways in the cold-resistant mutants. In conclusion, this study has identified cold-resistant yellow kiwifruit plantlets and cold-tolerance related genes. Moreover, the dataset got in this study advances our knowledge of the cold-adaptive genes in the regulatory networks and leads to understand the cold tolerance mechanisms in the tetraploid yellow kiwifruit.
Effect of activity level after TKA on the relative bone mineral density measured on standard radiographs in periprosthetic tibial bone: a five-year follow-up study
Authors: Liu Yong , He Peiheng , Li Xing , Ye Yongheng , Liu Minghao , Chen Weizhi , XU Dongliang 
Published: January 2020

Abstract
Background: To suggest the best activity level to guide clinical rehabilitation by evaluating the effect of activity level on BMD in the proximal tibia 5 years after TKA.Methods: In 121 patients, the UCLA activity rating was evaluated, and the relative BMD in periprosthetic tibial bone was measured by ImageJ software on anteroposterior X-ray images. ANOVA, chi-squared test, paired t test and nonlinear regression were used for data analysis.Results: Activity level significantly affected rBMD in the proximal tibia, with the smallest reduction in rBMD observed with moderate activity. The difference in rBMD% between the lateral and medial metaphysis was significant. The regions with a significant difference in rBMD% between the lateral and medial metaphysis were closest to the base plate of the prosthesis. The lateral and medial regions closest to the stem of the prosthesis showed no significant difference in rBMD%.Conclusions: Moderate-intensity functional exercise is recommended within 3 years after TKA.
Trabeculae microstructure parameters serve as effective predictors for marginal bone loss of dental implant in the mandible
Authors: Zhang Hengguo , Shan Jie , Zhang Ping , Jiang Hongbing 
Published: January 2020

Abstract
Background : To investigate the effectiveness and feasibility of machine learning models based on trabecular microstructure parameters for predicting the occurrence of marginal bone loss (MBL) of the submerged dental implant in mandible. Methods : Clinical variables and morphological parameters of trabecular bone were collected from 81 subjects with submerged implants in the mandible (41 cases of abnormal MBL and 40 as normal controls). We measured the peri-implant MBL level by a cone-beam computed tomography (CBCT) at the follow-up of 20.95±2.67 months after functional loading. The morphological parameters and possible factors associated with MBL were collected in a mean of 3.98±1.06 months at the early loading stage. All variables were analyzed using correlation and covariance matrices. Support vector machine (SVM), artificial neural network (ANN), logistic regression (LR) model and random forest (RF) were actualized to predict abnormal MBL. Results : At the early stage of functional loading, the abnormal MBL cases showed a significant increase of structure model index (SMI) and trabecular pattern factor (Tb.Pf) in peri-implant. Meanwhile, SMI and Tb.Pf simultaneously revealed a significantly high positive correlation with MBL. The LR model exhibited the best outcome in predicting MBL (AUC = 0.956), followed by SVM (AUC = 0.928), RF (AUC = 0.917), ANN (AUC = 0.900), SMI alone (AUC = 0.705) and Tb.Pf alone (AUC = 0.663). Compared with one single predictor, all algorithm models yielded significantly superior performance. Conclusion : Abnormal MBL cases demonstrated the premonitory morphological variation in trabecular bone at the early stage. MBL prediction could be achieved by machine learning methods.
Addition of Diffusion-Weighted Imaging to Dynamic Contrast Enhanced MRI: can it help differentiate benign from malignant jaw lesions?
Authors: Zhao Xue 
Published: January 2020

Abstract
Objective: To explore the diagnosis value of adding diffusion-weighted imaging (DWI) to dynamic contrast enhanced MRI (DCE-MRI) in distinguishing benign from malignant jaw lesions.Materials and Methods: This retrospective study was involved 53 patients (23 benign, 30 malignant) with jaw lesions confirmed by pathology were analyzed. DWI and DCE-MRI were performed in all patients. The Apparent Diffusion Coefficient (ADC) value as well as the DCE-MRI parameters [time to peak (Tpeak), wash in rate (WIR), wash out rate (WOR), relative enhancement, and maximum enhancement] were measured for each patient. The optimal cut-off value of ADC values and DCE-MRI parameters were determined by using a receiver operating characteristics (ROCs), and the area under ROC curve (AUC) was evaluated. P < 0.05 was considered to indicate a significant difference. Results: Among the five parameters of DCE-MRI, the WOR displayed the most significantly difference with a threshold value of 4.90 l/s between benign and malignant group (P<0.05). When only WOR was used as the basis for diagnosis, the sensitivity, specificity, and AUC were 77.20%, 78.00%, and 0.800, respectively (Fig.3, 4). However, sensitivity (77.20% vs 83.30%), specificity (78.00% vs 87.20%) and accuracy (0.80 vs 0.85) significantly improved with the addition of ADC values in the evaluation of jaw lesions, which manifested better than that by using WOR alone.Conclusion: The high WOR of DCE-MRI may have a malignant tendency, but in the evaluation of jaw disease, the addition of ADC value can improve the diagnostic value.
Prehospital risk stratification in non-ST-elevation acute coronary syndrome; a paramedic echocardiography pilot study.
Authors: Jacobsen Lars , Jortveit Jarle , Moe Espen Ellingsen , Olsen Roy Bjørkholt , Dahlslett Thomas , Grenne Bjørnar , Thorsen Kjetil , Bache Kristi Cecilie Grønvold 
Published: January 2020

Abstract
Background Prehospital risk stratification and timing of revascularization in Acute Coronary Syndromes (ACS) is currently based on the electrocardiogram (ECG). ST- elevation in the ECG indicates myocardial infarction (STEMI) in need of immediate reperfusion therapy. However, a large group of ACS patients presents without ST-elevation in ECG, despite coronary occlusions. In these high risk non-ST-elevation myocardial infarctions (NSTEMI), immediate reperfusion may be just as crucial for the prognosis, but prehospital diagnostic tools to identify them are lacking. Objective This pilot study investigated if focused prehospital transthoracic echocardiography (TTE) images achieved by paramedics could be transferred to the in-hospital cardiologist for diagnostic evaluation, and test if this, in combination with a point-of-care (POC) high-sensitive Troponin-T (Hs-cTnT) test, facilitates prehospital identification of high risk NSTE-ACS. Methods Paramedics were trained to obtain focused prehospital TTE images and provide POC Hs-cTnT - testing in patients with suspected ACS. The information was transferred to the in-hospital cardiologist who determined the treatment strategy by evaluating the information provided by the paramedics. Results 123 patients were included between November 30 th 2017 and November 30 th 2018. TTE images were interpretable in 107 cases (87%), and Hs-cTnT measures in 113 cases (93%). NSTEMI was the final diagnosis in 16 patients (13%), where 13 got admitted directly for percutaneous coronary intervention (PCI). These patients bypassed the local hospital and saved time to treatment based on the prehospital detection of NSTE-ACS. Conclusion Interpretable focused TTE images and POC Hs-cTnT measures can be achieved by paramedics in most patients with suspected NSTE-ACS. This information seems to aid in-hospital cardiologist admitting the right patients to the PCI hospital. The study strongly indicates that prehospital diagnostics and risk assessment of patients with suspected NSTE-ACS is feasible. A larger study is needed to clarify the diagnostic accuracy of focused prehospital TTE in suspected NSTE-ACSTrial registration Clinical trials NCT04223986, ID: 17/05178-2 – 522). Registered retrospectively September 23rd 2018. https://clinicaltrials.gov/show/NCT04223986.
A new anatomic zone division within the cervical spinal canal
Authors: Liu Yadong , Liu Li , Kong Chao , Chen Xueming , Yuan Xin , Gao Yan , Guan Yun 
Published: January 2020

Abstract
Background: Detailed anatomical information is important for identifying the origin and anatomic basis of symptoms in cervical spondylosis patients, but very little quantitative data have been reported. This study characterized the morphologic features of cervical spinal nerve rootlets and defined different zones at cervical spinal canal to provide an anatomic basis for studying cervical spondylosis. Methods: Ten cervical spines from C2 to T1 were obtained for the present study. We defined three zones from midline to lateral part (zone I, II and III) and two zones from cranial to caudal (zone P and zone IP) on the coronal plane within the cervical spinal canal. Quantitative anatomy of the zones at different cervical segments were measured including: 1) horizontal widths of zone I, II and III; 2) Length of the cervical spinal cord segment at the ventral rootlets (LV); 3) The pedicle height (PH, zone P) and the inter-pedicle height (IPH, zone IP); 4) The distance between the superior margin of pedicle and the exit of the uppermost ventral nerve rootlet (PN). Results: There was a trend that the horizontal widths of zone I gradually decrease from C4 to C8 but without significant differences (P=0.98). The value of zone II at C4 was significantly less than that at other levels (P=0.008). The value of zone III increases from C4 to C8, and the values at C7 and C8 were significantly higher than those at C4, C5 and C6 (P=0.032). PHs and IPHs were not significantly different between different levels (P1=0.365; P2=0.240). The values of LV at C4 and C8 were smaller than those of C5, C6 and C7 (P=0.001). The value of PN showed an increasing trend. At C4, the uppermost ventral rootlet was at about the same height as C3 pedicle, while C8 uppermost ventral rootlet was at the same level as the inferior part of C6 pedicle. Ventral intradural intersegmental connections were found in three intersegments (two specimens) out of 20 intersegments (15%). Conclusions: The current definition of anatomic zones may be useful for an accurate diagnosis of cervical spondylosis and a safe and effective anterior decompression surgery.
A novel internal heat recycling concept for reducing energy consumption and increasing flux through three-stage air-gap–water-gap membrane distillation system
Authors: Abu-Zeid Mostafa Abd El-Rady , Lu Xiaolong , Zhang Shaozhe 
Published: January 2020

AbstractThe low flux and high energy consumption problems of the conventional three-stage air-gap membrane distillation (AG-AG-AG)MD system caused by the low temperature difference between hot and cold feed at both sides of the membrane and high boundary layer thickness were solved successfully by replacing one of the three stages of air gaps by a water gap. The novel three-stage air-gap–water-gap membrane distillation (AG-AG-WG)MD system reduced energy consumption and increased flux due to efficient internal heat recycling by virtue of a water-gap module. Heat and mass transfer in novel and conventional three-stage systems were analyzed theoretically. Under a feed temperature of 45 °C, flow rate of 20 l/h, cooling temperature of 20 °C, and concentration of 340 ppm, the (AG-AG-WG)MD promoted flux by 17.59% and 211.69%, and gained output ratio (GOR) by 60.57% and 204.33% compared with two-stage (AG-WG)MD and one-stage AGMD, respectively. This work demonstrated the important role of a water gap in changing the heat and mass transfer where convection heat transfer across the water gap is faster by 24.17 times than conduction heat transfer through the air gap. The increase in flux and GOR economized the heating energy and decreased waste heat input into the system. Additionally, the number of MD stages could increase the achieving of a high flux with operation stability.
Virulence Factors of the Entomopathogenic Genus Metarhizium
Authors: González-Hernández Gloria A. , Padilla-Guerrero Israel E. , Martínez-Vázquez Azul , Torres-Guzmán Juan C. 
Published: January 2020

:
The fungal genus Metarhizium has been used as an entomopathogen worldwide for
approximately 140 years, and its mechanism of infection and its virulence factors have been studied.
The present review is a compilation of virulence factors described in the literature to date and
their participation in specific stages of the infection process.
Investigation on the Preparation, Characteristics, and Controlled Release Model of Paeonol-Loaded Liposome in Carbomer Hydrogel
Authors: Liu Qinqin , Xia Hongmei , Xu Yinxiang , Cheng Yongfeng , Cheng Zhiqing 
Published: January 2020

Objective:
Paeonol is a phenolic compounce that is volatile. In order to decrease its volatility
and achieve controlled release, paeonol-loaded liposome in carbomer hydrogel was prepared by coating
with soybean phospholipid via ethanol injection method and then added into the carbomer hydrogel.

Methods:
The quality of paeonol-loaded liposome in carbomer hydrogel was evaluated by the degree of
roundness, particle size distribution, zeta potential, entrapment efficiency (filtration method and chitosan
neutralization method), viscosity, infrared spectrum, etc. Furthermore, the diffusion from paeonolloaded
liposome in hydrogel was studied in vitro.

Results:
The results showed that the average particle size of paeonol-loaded liposome was about 401
nm, the potential was -17.8 mV, and the entrapment efficiency was above 45%. The viscosity of paeonol-
loaded liposome in hydrogel was 23.972×10-3 Pa*s, and the diffusion rate from paeonol-loaded
liposome in hydrogel in vitro was obviously slower than that from the other paeonol preparations.

Conclusion:
The conclusions could be drawn that paeonol-loaded liposome in hydrogel was a kind of
novel preparation, and its diffusion in vitro had obvious controlled-release characteristics, which further
proved that it might improve the bioavailability of paeonol.