bluuebunny/crossref_metadata_2025_split huggingface dataset:
Substrate Specificity of the TRAMP Nuclear Surveillance Complexes
Authors: Delan-Forino Clémentine , Spanos Christos , Rappsilber Juri , Tollervey David 
Published: March 2020

ABSTRACTDuring nuclear surveillance in yeast, the RNA exosome functions together with the TRAMP complexes. These include the DEAH-box RNA helicase Mtr4 together with an RNA-binding protein (Air1 or Air2) and a poly(A) polymerase (Trf4 or Trf5). To better determine how RNA substrates are targeted, we analyzed protein and RNA interactions for TRAMP components. Mass spectrometry identified three distinct TRAMP complexes formedin vivo. These complexes preferentially assemble on different classes of transcripts. Unexpectedly, on many substrates, including pre-rRNAs and pre-mRNAs, binding specificity was apparently conferred by Trf4 and Trf5. Clustering of mRNAs by TRAMP association showed co-enrichment for mRNAs with functionally related products, supporting the significance of surveillance in regulating gene expression. We compared binding sites of TRAMP components with multiple nuclear RNA binding proteins, revealing preferential colocalization of subsets of factors.TRF5deletion reduced Mtr4 recruitment and increased RNA abundance for mRNAs specifically showing high Trf5 binding.
Grain aphids (Sitobion avenae) with knockdown resistance (kdr) to insecticide exhibit fitness trade-offs, including increased vulnerability to the natural enemyAphidius ervi.
Authors: Little Damon , Malloch Gaynor , McNamara Louise , Jackson Gail E. 
Published: March 2020

AbstractThe development of insecticide-resistance mechanisms in aphids has been associated with inhibitory, pleiotropic fitness costs. Such fitness costs have not yet been examined in the UK’s most damaging cereal aphid,Sitobion avenae(grain aphid) (Hemiptera: Aphididae). This study aimed to evaluate the fitness trade-offs of the insecticide-resistantS. avenaeclone versus an insecticide-susceptibleS. avenaeclone. Additionally, the parasitoid,Aphidius ervi(Hymenoptera: Braconidae), was introduced to examine its potential as a biological control agent. This study found that insecticide-resistant clones had significantly lower population growth and individual relative growth rate. Furthermore, insecticide-resistant clones suffered from a significantly greater rate of parasitisation (mummification) compared to their insecticide-susceptible counterparts. The successfulness of the parasitoid as a biological control agent could prevent the spread of the insecticide-resistant genotype. However, for this to be possible, insecticide spraying regimes need to be moderated, and habitat modification and parasitoid manipulation must be considered.
Recombinant Protein IGF1-24 Stimulates Rat Cardiomyocytes Proliferation and Repairs Myocardial Injury
Authors: Wang Xing , Fu Manxue , Yi Qian , Feng Jianguo , Liao Yi , Xu Xichao , Chen Ying , Zhang Lu , Sun Huifang , Liu Piaoyang , Liang Yuanyuan , Tang Liling 
Published: March 2020

AbstractMammal cardiomyocytes lose their ability of regeneration shortly after birth. Reduced cardiomyocytes number caused by myocardial damage is unable to reverse in current clinical therapies. Therefore, it is important and urgent to find new approaches to stimulate cardiomyocytes regeneration. Here we design a recombinant protein IGF1-24 and show that it triggers cardiomyocytes proliferation in rat. 7 days after tail intravenous injection of IGF1-24, 6-7-weeks-old healthy rats showed marked improvements in cardiomyocytes proliferation. Next, we injured rats cardiac with isoproterenol and treated them with IGF1-24 injection. We found that it efficiently induced cell proliferation with significant improvements in heart histology. These results show that the recombinant IGF1-24 stimulates cardiomyocytes proliferation and can be used to achieve cardiac repair through stimulating endogenous cardiomyocyte proliferation in rats. The IGF 1-24 could be a prospective medicine to heart repair because it has high efficiency in triggering cell proliferation and it can be easily applied to heart by intravenous injection
STK32A is a dual-specificity AGC kinase with a preference for acidic substrates
Authors: Sorrell Fiona J. , Miranda Fabrizio , Abdul Azeez Kamal R. , Chaikuad Apirat , Kettenbach Arminja N. , Gerber Scott A. , Knapp Stefan , Ahmed Ahmed A. , Elkins Jonathan M. 
Published: March 2020

AbstractThe STK32 kinases are a small subfamily of three uncharacterised serine/threonine kinases from the AGC kinase family whose functional role is so far unknown. Here, we analyse the consensus peptide sequence for STK32A phosphorylation, showing that STK32A is directed towards acidic substrate sequences and exhibits dual-specificity for serine/threonine and tyrosine residues. A crystal structure of STK32A reveals an overall structure typical of the AGC protein kinase family but with significant and unique features including an altered binding mode of the hydrophobic motif to the N-terminal lobe of the kinase domain, and a novel alpha-helix in between the turn motif and the hydrophobic motif. The crystal structure combined with phylogenetic analysis reveals the evolutionary conservation of the acidic substrate preference.In vitrobinding assays demonstrated that the STK32 kinases bind significant numbers of clinically used kinase inhibitors.
figureComposer: A web-based interactive multi-panel bio-infographic designing tool
Authors: Li Kejie , Hurt Jessica , Whelan Christopher D. , Challa Ravi , Lin Dongdong , Zhang Baohong 
Published: March 2020

AbstractBackgroundMany fit-for-purpose bioinformatics tools generate plots to investigate data quality and illustrate findings. However, assembling individual plots in different formats from various sources into one high-resolution figure requires mastery of commercial tools or even programming skills. In addition, it is a time-consuming and sometimes frustrating process even for scientists with modest computational skills.ResultsWe developed figureComposer, a web-based bioinformatics tool that interactively arranges high-resolution images in various formats, mainly SVG to produce one multi-panel publication-quality composite figure in both PDF and interactive HTML formats in a user-friendly matter, requiring no programming skills.ConclusionsfigureComposer is open-source and publicly available web tool that can be accessed online athttps://baohongz.github.io/figureComposerwhile the source code is provided athttps://github.com/baohongz/figureComposer.
Structural investigation of Mycobacterial protein complexes involved in the stationary phase stress response
Authors: Kirykowicz Angela M , Woodward Jeremy D 
Published: March 2020

AbstractLarge protein complexes play key roles in mediating biological processes in the cell. Little structural information is known on the protein complex mediators governing persistence in the host forMycobacterium tuberculosis(Mtb). We applied the ‘shotgun EM’ method for the structural characterisation of protein complexes produced after exposure to stationary phase stress for the model Mycobacterium,M smegmatis(Msm). We identified glutamine synthetase I, essential forMtbvirulence, in addition to bacterioferritin, critical forMtbiron regulation, and encapsulin, which produces a cage-like structure to enclose target proteins. Further investigation found that encapsulin carries dye-decolourising peroxidase (DyP), a potent protein antioxidant, as the primary cargo during stationary phase stress. Our ‘proof-of-concept’ application of this method offers insight into identifying potential key-mediators inMtbpersistence.
DBT and intellectual disabilities: the relationship between treatment fidelity and therapeutic adaptation
Authors: Patterson Christopher , Williams Jonathan , Jones Robert S.P. 
Published: March 2020

Purpose
There is growing literature on the application of Dialectical Behaviour Therapy (DBT) with adults with intellectual disabilities (IDs). To draw upon the evidence-base from mainstream approaches, adapted interventions must remain true to their theoretical foundations and retain key components. The purpose of this paper was to establish the extent to which DBT has been adapted for adults with ID, and whether existing adapted protocols can still be considered DBT.

Design/methodology/approach
The theoretical underpinnings and key components of DBT were identified. Six DBT studies were critiqued according to these criteria.

Findings
In terms of content, only one intervention comprised all necessary elements. All of the remaining interventions included a skills group; two included individual therapy and another two included group consultation. None of the remaining interventions provided 24-h telephone support. Furthermore, none of the studies explicitly described using dialectical strategies.

Originality/value
To the best of the authors’ knowledge, this is the first paper to critically examine the evidence-base for the use of DBT in ID, particularly its fidelity.
A benchmarking framework for understanding bus performance in the US
Authors: Morse Lindsey , Trompet Mark , Barron Alexander , Anderson Richard , Graham Daniel J. 
Published: March 2020

PurposeThis paper describes a benchmarking framework applied to medium-sized urban public bus agencies in the United States, which has overcome the challenges of data quality, comparability, and understanding.Design/methodology/approachThe benchmarking methodology described in this paper is based on lessons learned through seven years of development of a fixed-route key performance indicator (KPI) system for the American Bus Benchmarking Group (ABBG). Founded in 2011, the ABBG is a group of public medium-sized urban bus agencies that compare performance and share best practices with peers throughout the United States. The methodology is adapted from the process used within international benchmarking groups facilitated by Imperial College and consists of four main elements: peer selection, KPI system development, processes to achieve high-quality data, and processes to understand relative performance and change.FindingsThe four main elements of the ABBG benchmarking methodology consist of 18 subelements, which when applied overcome three main benchmarking challenges: comparability, data quality, and understanding. While serving as examples for the methodology elements, the paper provides specific insights into service characteristics and performance among ABBG agencies.Research limitations/implicationsThe benchmarking approach described in this paper requires time and commitment and thus is most suitably applied to a concise group of agencies.Practical implicationsThis methodology provides transit agencies, authorities, and benchmarking practitioners a framework for effective benchmarking. It will lead to high-quality comparable data and a strong understanding of the performance context to serve as a basis for organizational changes, whether for policy, planning, operations, stakeholder communication, or program development.Originality/valueThe methodology, while consistent with recommendations from literature, is unique in its scale, in-depth validation and analysis, and holistic and multidimensional approach.
IdeaChain: a conceptual proposal for blockchain-based STI policy development
Authors: Burmaoglu Serhat , Saritas Ozcan , Sesen Harun 
Published: March 2020

Purpose
The purpose of this study is to discuss the possibility of setting up a platform for inclusive policymaking process drawing upon the blockchain concept. The study posits that blockchain also has great potentials in non-financial applications, such as in policymaking, where there is a need for bottom-up approaches with more decentralized, distributed and evidence-based processes.

Design/methodology/approach
The study makes use of an analogy-based creative design methodology. The design science paradigm has its roots in engineering and the sciences of the artificial (Simon, 1996). As a problem-solving paradigm for solving complex engineering issues, design science seeks to create innovations that define the ideas, practices, technical capabilities and products through which the analysis, design, implementation and use of information systems can be effectively and efficiently accomplished. In the present study, the policy development theories and the logic of blockchain are synthesized to prepare a task model for the “IdeaChain” concept as a platform for creating, sharing and validating novel ideas as well as converting them into policies or new ventures through the funding mechanisms.

Findings
The IdeaChain concept is designed and demonstrated through its use in the domain of science, technology and innovation (STI) policy, which can be extended to cover all innovative activities linking the whole process from their emergence, funding, development, implementation and impact upon policy.

Originality/value
Blockchain is mostly discussed in literature with its impact on financial sector. IdeaChain is the first attempt to explore the potentials of blockchain in STI policymaking.
Is there a place for gender questions in studies on entrepreneurship, or for entrepreneurship questions in gender studies?
Authors: Holmquist Carin , Sundin Elisabeth 
Published: March 2020

Purpose
This paper aims to add to the diversity of gender and entrepreneurship studies by presenting the (lived experience) perspective on the development of research on women as entrepreneurs.

Design/methodology/approach
An essay built on personal reflections on the development of the field since the 1980s.

Findings
Research on entrepreneurship has shifted toward quantitative studies and the paper format, leading to fragmented research. Research on gender shows another trend, where empirical data have become less central – “women” as individuals are to a large extent not discussed. The authors conclude that the field of gender and entrepreneurship, therefore, is a fruitful arena to perform research in as long as the physical women are not neglected.

Originality/value
Building on the lived experience for almost 40 years as researchers of women as entrepreneurs, the perspective contributes to the understanding of the development of the field.
Analyzing the formation of a paradoxical organizational identity
Authors: Felix Bruno 
Published: March 2020

PurposeThis study aims to understand the construction process of an organizational identity in a hybrid organization.Design/methodology/approachThe author developed a single case study based on in-depth interviews, non-participant observations and document analysis in a credit cooperative.FindingsAfter periods of changes in organizational identity (from idealism to pragmatism), the formation of a paradoxical organizational identity was observed in which the core value became a central polarity between idealism and pragmatism; after periods when members engaged in actions that promoted resistance or change, they framed past events in a story of stable dynamics between idealism and pragmatism; and pro-distinctiveness and -similarity forces in relation to other organizations were reconciled in a quest for optimal distinctiveness that simultaneously enabled the development of uniqueness and adequacy.Originality/valueThis is the first study to adopt a paradox perspective to analyze the identity of a cooperative.
Research and improvement of fraud identification model of Chinese A-share listed companies based on M-score
Authors: Lu Wanting , Zhao Xiaokang 
Published: March 2020

Purpose
The purpose of this paper is to start with the background of the construction of the M-score model, find the variables that can represent the fraud characteristics of Chinese companies, and use the data of Chinese A-share listed companies to modify the M-score model.

Design/methodology/approach
In this paper, the fraud behavior of Chinese enterprises that M-score cannot detect is summarized as the basis of adding variables. Then, based on the data of Chinese listed companies, a modified M-score model including nine variables is constructed by the logistic regression method based on Wald.

Findings
Based on the original 8 variables of M-score, this paper adds 10 new variables that can represent the fraud characteristics of Chinese listed companies, and finally, constructs a modified M-score model with 9 variables. Results indicated that indexes such as gross profit margin, fixed assets depreciation rate, equity concentration and audit opinion can characterize the financial fraud of Chinese listed companies.

Practical implications
The modified M-score model based on the characteristics of Chinese enterprises’ fraud is more suitable for Chinese market, which can help investors avoid fraud risks, protect their own rights and interests and reduce losses.

Originality/value
Starting from the background of the model, this paper looks for variables that can characterize the characteristics of fraud in Chinese listed companies. Then, subdivides the research samples into specific fiscal years in which fraud occurs, so that the modified M-score model can be more suitable for the Chinese market.
Does the efficiency of corporate governance and intellectual capital affect a firm's financial distress? Evidence from Egypt
Authors: Shahwan Tamer Mohamed , Habib Ahmed Mohamed 
Published: March 2020

PurposeUsing data on 51 firms traded in the Egyptian Exchange from 2014 to 2016, this paper aimed to assess the efficiency of corporate governance (CG) and intellectual capital (IC) practices and to explore their influence on the probability of a firm's financial distress.Design/methodology/approachThe relative efficiency of CG and IC practices has been measured under the Malmquist data envelopment analysis model. A modifiedZ-score model was applied to assess firms' financial distress.FindingsThe Wilcoxon signed-rank test revealed almost insignificant evidence regarding the improvement of CG and IC efficiency over the study period. The efficiency score of CG practices had no impact on the likelihood of financial distress. However, the efficiency score of IC negatively affected the probability of financial distress.Research limitations/implicationsThe integration of data envelopment analysis with Tobit regression was required for identifying the significant drivers of efficient CG and IC.Practical implicationsThe findings shed light on the role of CG and IC in alleviating the degree of financial distress in Egypt as an emerging market, especially the need to raise firms' compliance with the Egyptian CG code from a voluntary to mandatory status.Originality/valueThis study, using Malmquist data envelopment analysis, is among the first attempts to assess the relative efficiency of CG and IC practices and their effects on financial distress.
Antecedents and outcome of commitment in Islamic banking relationships – an emerging African market perspective
Authors: Roberts-Lombard Mornay 
Published: March 2020

Purpose
This study aims to investigate how the commitment of Islamic banking customers is influenced by trust, relationship expectations and conflict management. In turn, the influence of their commitment on future satisfaction is determined.

Design/methodology/approach
A descriptive research design was applied and responses were obtained from Islamic banking customers through the application of self-administered questionnaires. A total of 350 completed questionnaires were used in analysing the data. An exploratory factor analysis established the interrelationships of the scales used to measure the study’s constructs. In addition, both the measurement and structural models were evaluated.

Findings
Trust and relationship expectations significantly and positively influence customer commitment, while conflict management has no significant influence on the commitment of Islamic banking customers to their bank. In addition, commitment significantly and positively influences the satisfaction experiences of Islamic banking customers.

Research limitations/implications
The tested model validates the hypothesised relationships between the trust, relationship expectations, commitment and satisfaction of Islamic banking customers. However, the relationship between conflict management, commitment and satisfaction was not established. Commitment is linked to trust and relationship expectations, as well as its outcome, satisfaction. However, commitment could not be linked to antecedent conflict management.

Practical implications
The findings could assist retail banks servicing Islamic banking customers in offering in-depth knowledge of how trust and relationship expectations can foster customer commitment, eventually securing the positive satisfaction of customers.

Originality/value
The study focussed on Islamic banking customers and determined the interrelationships between commitment and related constructs. Few studies, however, have examined how commitment relates to its precursors and outcome from an Islamic banking perspective in an emergent African economy.
Journey of neuroscience: marketing management to organizational behavior
Authors: Chattopadhyay Rachana 
Published: March 2020

PurposeThe purpose of this paper is to provide an overview of the advancement of neuroscience research works in the domains of marketing management and organizational behavior and its future scope for expansion in the area of organizational behavior.Design/methodology/approachA total of 77 neuroscience research articles in the area of marketing management and organizational behavior published between 2004 and 2017 were reviewed, and a possible future direction for neuroscience research in the area of organizational behavior was identified in this article.FindingsFindings from neuroscience research works suggest that tools and techniques that are useful in the neuroscience domain are also quite powerful and reliable in the context of organizational behavior research. Here, it should be noted that not all of these are independently powerful. Therefore, in certain cases, it is desirable to use neuroscience techniques in association with existing methods.Originality/valueNeuroscientific research works in the context of the marketing domain were started with the motivation to identify the neural signaling in association with different marketing initiatives. However, the research works have proceeded much deeper and entered into the field of consumer psychology. Further research shows that neuroscience techniques are quite useful in the understanding of consumer behavior and can be extended in the field of organizational behavior. In this study, the authors have provided the future direction of neuroscience research works in the area of organizational behavior.