bluuebunny/crossref_metadata_2025_split huggingface dataset:
Agro Informatics with its Various Attributes and Emergence: Emphasizing Potentiality as a Specialization in Agricultural Sciences—A Policy Framework
Authors: Paul P. K. , Aithal P. S. , Sinha R. R. , Saavedra Ricardo , Aremu Bashiru 
Published: July 2020

Informatics is one of the important branches of Applied Sciences. It is a practicing area and also a field of study. The applications of Informatics in different areas and knowledge field has created various subjects and among these, one of important and emerging is Agricultural Informatics; which is the Information Science and Technology applications in the agriculture as well allied areas. In other words, techniques and technologies of both the fields i.e. Informatics and Agriculture lead the birth of Agro Informatics. The management and analysis of agricultural data with the help of Computing and IT may also be called as Agricultural Informatics. There are many allied nomenclatures of the field, but all are related and closely connected. In short, it is also called as Agro Informatics. Initially, it was treated only as a practicing area in the agriculture, horticulture, and veterinary sciences; but gradually it is a study area with different levels of programs at various universities and institutions, internationally. However, in the developed nation it is more common and widely available. Agro Informatics is an interdisciplinary field and very diverse. There are many features and functions, roles due to its timely need. This is a conceptual and policy-based research hence various aspects of Agro Informatics including its feature, functions, stakeholders, technologies including allied branches are mentioned. The paper also highlighted the proposed and possible programs of this field in academics in the field of Agricultural Sciences, as an empirical and policy research.
Foaming Agent Developed for Gas Wells with Liquid Loading Problem Using New Surfactant and Nanotechnology
Authors: Wu Junwen , Jia Wenfeng , Xian Chenggang 
Published: July 2020

Summary
Liquid buildup in the wellbore is one of the major causes of production decline in gas wells, in which case additional energy is needed to drain off the liquid to solve this problem.
Foaming agents offer a method to reduce the liquid density to make it easier to be lifted with the gas flow, unloading the accumulated liquid in gas wells. The main ingredient of foaming agents are surfactants. Foam stability is influenced by several factors, such as salinity, temperature, and pressure, so a foaming stabilizer is usually needed for a foam system. A foaming agent should be developed to form stable foams in the presence of a salt or sweet-water hydrocarbon phase at a given temperature and pressure.
Recently, various kinds of foaming agents have been developed and discussed. Previous studies mainly focused on the complex interaction between an anionic surfactant and amphoteric ion surfactant; however, stability of the foam system formed by these foaming agents needs to be further improved (Nikolai et al. 2009). Therefore, development of a novel foaming agent with improved stability is necessary, especially for the application under downhole conditions. The complex interaction between the anionic and cationic surfactants is neglected in previous research. For example, the synergies between the anionic and cationic surfactants with appropriate methods can greatly improve the foam stability compared with the one-component system. A complex phase behavior and microstructure that has a high surface activity and foam stability can be obtained by the strong electrostatic interaction between the opposite charge ionic head groups and the hydrophobic interaction between the hydrocarbon groups. A gemini surfactant with a spacer can make the molecules pack tighter and increase the surfactant cohesion within the monolayer, which can greatly enhance the foam stability. The liquid film of foam formed by the surfactant is dynamically unstable because the liquid film cannot prevent the diffusion of gas, and the foam will burst quickly. However, solid films with particles adsorbed in the gas/water interface can weaken the foam drainage speed, so that the foam stability is greatly enhanced.
In summary, a robust foaming agent is developed with the introduction of an anionic-nonionic surfactant complexed with a gemini cationic surfactant; moreover, nanoparticles with a certain hydrophilicity and size are also adopted as stabilizers.
An Empirical Analysis of the Socioeconomic and Demographic characteristics influencing Birth Certification in Nigeria
Authors: Anaduaka Uchechi Shirley 
Published: July 2020

Abstract
Background: Promoting birth certification is instrumental to achieving target 16.9 of the Sustainable Development Goals: legal identity for all by 2030. However, limited research has investigated the determinants of birth certification of children in the sub-Saharan African context. This study analyzes the socioeconomic and demographic factors associated with the birth certification of children under-five years in Nigeria. Methods: The study employed three rounds of the Nigerian Demographic and Health Survey involving 79487 children (0-4 years) in Nigeria. Birth certification was defined as whether a parent/caregiver had a child’s birth certificate at the time of the interview. Ordinary least squares and multilevel logistic regression models established the relationships between the socioeconomic and demographic factors and birth certification. Results: Children under-five years who had a skilled attendant at birth, at least one vaccination, and maternal access to prenatal visits had about 25.6%, 60.5% and 35.0% higher odds of having their births certified. Children born to more educated mothers and fathers had 1.023 and 1.012 times the odds of birth certification. Children from average and rich households also had 13.2% and 34.2% higher odds of birth certification, respectively. Conversely, child age, higher birth order, longer birth intervals, polygyny, having at least two dead siblings, father being employed in a low skilled job, living far from a registration center and in a poor community are risk factors for birth certification. Maternal age at birth and paternal has non-linear, albeit weak effects on birth certification. Being large at birth, bank account ownership were also significant predictors of birth certification. Mixed effects were noted for religion, ethnicity and region. Finally, no significant effects were noted for gender, maternal occupation and rural residence. Conclusions: The findings identify several socioeconomic and demographic factors associated with birth certification suggesting possible risks with improving birth certification in Nigeria. Access to health care and higher socioeconomic backgrounds are significant protectors of and distance to registration centers as obstacles to birth certification for children in Nigeria. Public policy strategies should encourage the use of health care services and also increase the number of registration centers in Nigeria.
High production of triterpenoids in Yarrowia lipolytica through manipulation of lipid components
Authors: Zhang Jin Lai , Bai Qiu Yan , Peng Yang Zi , Fan Jie , Jin Cong Cong , Cao Ying Xiu , Yuan Ying Jin 
Published: July 2020

Abstract
Background Lupeol exhibits novel physiological and pharmacological activities, such as anticancer and immunity-enhancing activities. However, cytotoxicity remains a challenge for triterpenoid overproduction in microbial cell factories. As lipophilic and relatively small-molecular compounds, triterpenes are generally secreted into the extracellular space. The effect of increasing triterpene efflux on the synthesis capacity remains unknown.Results In this study, we developed a strategy to enhance triterpene efflux through manipulation of lipid components in Y. lipolytica by overexpressing the enzyme Δ9-fatty acid desaturase (OLE1) and disturbing phosphatidic acid phosphatase (PAH1) and diacylglycerol kinase (DGK1). By this strategy combined with two-phase fermentation, the highest lupeol production reported to date was achieved, where the titer in the organic phase reached 381.67 mg/L and the total production was 411.72 mg/L in shake flasks, exhibiting a 33.20-fold improvement over the initial strain. Lipid manipulation led to a two-fold increase in the unsaturated fatty acid (UFA) content, up to 61%~73%, and an exceptionally elongated cell morphology, which might have been caused by enhanced membrane phospholipid biosynthesis flux. Both phenotypes accelerated the export of toxic products to the extracellular space and ultimately stimulated the capacity for triterpenoid synthesis, which was proven by the 5.11-fold higher ratio of extra/intracellular lupeol concentrations, 2.79-fold higher biomass accumulation and 2.56-fold higher lupeol productivity per unit OD in the modified strains. This strategy was also highly efficient for the biosynthesis of other triterpenes and sesquiterpenes, including α-amyrin, β-amyrin, longifolene, longipinene and longicyclene.Conclusions In conclusion, we successfully created a high-yield lupeol-producing strain via lipid manipulation. We demonstrated that the enhancement of lupeol efflux and synthesis capacity was induced by the increased UFA content and elongated cell morphology. Our study provides a novel strategy to promote the biosynthesis of valuable but toxic products in microbial cell factories.
Effects of calcitriol on oxidative burst, phagocytic function, and leukocyte cytokine production in shelter dogs
Authors: Jaffey Jared , Bessette Mariah , Tao Zenan , Bradley-Siemens Nancy , Thompson Melissa 
Published: July 2020

Abstract
Background

The active metabolite of vitamin D, calcitriol, has been shown across many different species to augment innate immune responses and dampen aberrant proinflammatory cytokine production. Community acquired infections are common in shelters and consume limited shelter resources, impact adoption rates, and can result in unnecessary euthanasia. Prophylactic oral vitamin D supplementation decreases the incidence and severity of upper and lower respiratory tract infections in humans. Before a clinical trial investigating the clinical benefit of oral vitamin D supplementation in shelter dogs can be pursued, an in vitro study evaluating the immunomodulatory effects of calcitriol in blood from shelter dogs is warranted. Therefore, the objective of this study was to determine if incubation of whole blood obtained from apparently healthy dogs housed in a shelter for ≥ 7 days with calcitriol would alter granulocyte/monocyte (GM) oxidative burst and phagocytic function as well as pathogen-associated molecular pattern (PAMP)-stimulated leukocyte production of tumor necrosis factor (TNF)-α, and interleukin (IL)-6, and IL-10.

Results

Ten dogs housed in a shelter for ≥ 7 days were enrolled in a prospective cohort study. Whole blood from these dogs was incubated with calcitriol (10-7 M) or diluent (control) for 24 h. Subsequent to this incubation phagocytosis of opsonized-Escherichia coli (E. coli) and E. coli-induced oxidative burst were evaluated via flow cytometry. In addition, leukocyte production of TNF-α, IL-6, and IL-10 were measured using a canine-specific multiplex assay. Calcitriol significantly decreased leukocyte TNF-α production (p = 0.009) and increased IL-10 production (p = 0.002). Tumor necrosis factor-α-to-IL-10 ratio was significantly decreased with calcitriol (p = 0.017), while IL-6 production as well as GM oxidative burst and phagocytic function were not significantly affected.

Conclusions

These data indicate that calcitriol attenuates proinflammatory immune responses without affecting GM oxidative burst or phagocytic function in vitro in whole blood obtained from apparently healthy shelter dogs.
Blood Eosinophil in the Stable Period is a Biomarker of Frequent Exacerbations in Japanese Chronic Obstructive Pulmonary Disease Patients
Authors: Tashiro Hiroki , Kurihara Yuki , Takahashi Koichiro , Sadamatsu Hironori , Haraguchi Tetsuro , Tajiri Ryo , Takamori Ayako , Kimura Shinya , Sueoka-Aragane Naoko 
Published: July 2020

Abstract
BackgroundExacerbations are critical events in chronic pulmonary obstructive disease (COPD). The frequency of COPD exacerbations is associated with the prognosis, including mortality, but no useful biomarker has been established.MethodsThe present retrospective study investigated 481 COPD patients. Clinical features in the stable period were compared between patients who experienced severe exacerbation (n=88, 18.3%) and those who never experienced severe exacerbation (n=393, 81.7%). In the patients who experienced exacerbations, clinical features were also compared between frequent exacerbators (exacerbation rate ≥ 2 times/year, n=27, 30.7%) and infrequent exacerbators (1 time/year, n=61, 69.3%).ResultsCompared to COPD patients who never experienced exacerbations, body mass index (BMI), serum albumin, and pulmonary functions were significantly lower, and the cardiovascular disease comorbidity rate, COPD assessment test score, modified Medical Research Council dyspnea scale, and use of long-term oxygen therapy, long-acting β2 adrenergic agonist therapy, inhaled corticosteroid therapy, and macrolide therapy were significantly higher in COPD patients with exacerbations (all p < 0.01). In patients who experienced exacerbations, frequent exacerbators had significantly lower % forced expiratory volume in 1.0 second and a higher risk of critical exacerbations, percentage of blood eosinophils, history of mechanical ventilation use, and use of long-term oxygen therapy and of macrolide therapy than infrequent exacerbators (all p < 0.01). On multivariate analysis, the percentage of blood eosinophils was the parameter most correlated with exacerbation frequency (β value [95% confidence interval] 1.44 [1.11 – 1.86], p < 0.01).ConclusionBlood eosinophil in the stable period is the factor most correlated with the frequency of severe exacerbations.Trial registrationThe patients in this study was registered retrospectively
Risk Factors Of Post-Operative Sore Throat In Patients Undergoing Emergency Caesarean Section Under General Anaesthesia With Endotracheal Tube – A Prospective Cohort Study
Authors: Sasidharan Shibu , Shibu Babitha , Dhillon Harpreet , Singh Suneeta , Sinha Divya , Latheef Shifla Abdul 
Published: July 2020

Abstract
Background: A prospective cohort study to study the risk factors of post-operative sore throat (POST) in pregnant patients undergoing emergency caesarean section under general anaesthesia with endotracheal tube. Methods: 1000 pregnant patients were recruited to study the risk factors of POST over a period of 3 years. The data was analysed using SPSS-10 for Windows statistical software. Pearson’s Chi Square test was the statistical test of significance. Results: ETT size 7.0 decreased and ETT tube size 8.0 and above increased the risk for POST and hoarseness in the early postoperative period. The symptoms of POST surfaced mostly 8 hours after surgery (35.5%). A low cuff pressure decreased the incidence of POST. Incidence of POST increases with the duration of surgery. Conclusion: Incidence of POST in pregnant patients are comparable with general population and the factors that caused POST in general population caused the same in pregnant women, without any difference in characteristics, despite the complex change in physiology.
Proposal of a New Equation for Estimating Resting Energy Expenditure for Acute Kidney Injury Patients on Dialysis. A Machine Learning Approach.
Authors: ponce daniela , de Goes Cassiana Regina , de Andrade Luis Gustavo Modelli 
Published: July 2020

Abstract
Objective: The objective of this study was, therefore, to develop a new predictive equation of resting energy expenditure (REE) for acute kidney injury patients (AKI) on dialysis. Material and methods: A cross-sectional descriptive study has been carried out in 114 AKI patients on dialysis and mechanical ventilation consecutively selected, aged between 19 and 95 years. For the construction of the predictive model, 80% of the cases were randomly separated to train and 20% of unused cases for validation. Several machine learning models were tested in the training data: linear regression with Stepwise, rpart, support vector machine with radial kernel, generalized boosting machine, and random forest. The models were selected by 10-fold cross-validation and the performances evaluated by the root mean square error (RMSE). Results: There were 364 indirect calorimetry (IC) measurements in 114 patients, mean age of 60.65 ± 16.9 years and 68.4% were males. The average REE was 2081 ± 645 kcal. REE was positively correlated with C-reactive protein, minute volume (MV), expiratory positive airway pressure, serum urea, body mass index and inversely with age. The principal variables included in the selected model were age, BMI, use of vasopressors, expiratory positive airway pressure, minute volume, C-reactive protein, temperature, and serum urea. The final r-value in the validation set was 0.69. Conclusion: We propose a new predictive equation for estimating the REE for AKI patients on dialysis that use a non-linear approach with better performance than actual models.
Screening and Identification of Key Biomarkers in Breast Cancer with Brain Metastasis: Evidence from Bioinformatic Analysis
Authors: Shao tao ming , Hu zhi yang , Li wen wei , Pan long yun 
Published: July 2020

Abstract
Purpose. Breast cancer (BC) has a poor prognosis when brain metastases (BM) occur, and the treatment effect is limited. In this study, we aim to identify representative candidate biomarkers for clinical prognosis of patients with BM and explore the mechanisms underlying the progression of BC.Methods. Herein, we examined the Microarray datasets (GSE125989) obtained from the Gene Expression Omnibus database to find the target genes in BC patients with BM. We employed the GEO2R tool to filter the differentially expressed genes (DEGs) that participate in primary BC and BC with BM. Subsequently, using the DAVID tool, we conducted an enrichment analysis with the screened DEGs based on the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway and Gene Ontology (GO) functional annotation. The STRING database was employed to analyze the protein-protein interactions of the DEGs and visualized using Cytoscape software. Lastly, the Kaplan-Meier plotter database was employed to determine the prognostic potential of hub genes in BC.Results. We screened out 311 upregulated DEGs and 104 downregulated DEGs. The enrichment analyses revealed that all the DEGs were` enriched in the biological process of extracellular matrix organization, cell adhesion, proteolysis, collagen catabolic process and immune response. The significant enrichment pathways were focal adhesion, protein absorption and digestion, ECM-receptor interaction, PI3K-Akt signalling pathway, and Pathways in cancer. The top ten hub nodes screened out included FN1, VEGFA, COL1A1, MMP2, COL3A1, COL1A2, POSTN, DCN, BGN and LOX. The Kaplan-Meier plotter results showed that the three hub genes (FN1, VEGFA and DCN) are candidate biomarkers for clinical prognosis of patients with BM.Conclusion. we identified seven genes related to poor prognosis in BCBM. FN1, VEGFA and DCN can be considered as potential prognostic markers for BCBM. Meantime, COL1A1, POSTN, BGN and LOX may be linked to the distant transformation of BC.
Analysis of the Coping Ability and Mental Health of Medical Staff toward COVID-19 in Henan, China
Authors: Cui Songyang , Yin Lei , Shi Yuanyuan , Fan Mengying , Yang Xiu , Zhang Lulu , Li Linlin 
Published: July 2020

Abstract
Background Outbreak of COVID-19 imposed great pressure on the professional work and psychological health of medical staff, especially in the early stage. To assess the coping ability and mental health status of medical workers and find influencing factors on them during the early stage of the COVID-19 outbreak.Methods A cross-sectional study was conducted among medical staff in Henan from January 29th to February 4th with a total of 1739 participates involved. A self-made questionnaire used to evaluate the coping ability and mental health condition.Results The majority of the participants (96.8%) had coping ability scores (Median, 81; IQR, 75~87) above the passing line. The mental health scores of all the respondents were 58 (median; IQR, 44~68). Multivariate logistic regression analyses showed that older age, higher education level, and distrust in unofficial information were facilitating factors for better emergency copying ability among medical workers. For mental condition, medical workers had 3 promoting factors: older age, distrust of unofficial information, and not involved in the event. On the other hand, poor physical health was a risk factor. (P<0.05)Conclusions In the early stage, the overall coping ability and mental health of medical workers in Henan were relatively good, but the government needs to pay more attention to the coping capacity and mental health of medical workers who are younger, less educated, blind to unofficial information and in poor physical condition.
Second-child Policy and Incidence of Infant Eye Diseases in China
Authors: Ma Wenhui , Wan Xiaomei , Jiang Mingming , Ding Yichao , Wang Yuan , Zhang Jing 
Published: July 2020

Abstract
Purpose

To explore the influence of parental factors associated with the second-child policy on the incidence of ophthalmic diseases in infants.
Methods

Data were collected on children (aged < 3 years) admitted to Qingdao Eye Hospital, China, from January 2017 to December 2019, and patients with a family history of eye illnesses were excluded. We recorded the age, sex, diagnosis, mother’s history, age of the mother at birth, and parents’ education and the same values for all siblings.
Results

A total of 383 children (aged < 3 years) were admitted to the Qingdao Eye Hospital. There were also 159 siblings for a total of 541 children from 383 families. There were 229 single-child families and 154 multiple-child families. Among all the children involved in the study, the prevalence of ophthalmic diseases was 62.2% in the oldest child, 90.8% in the second, and 100% in the third. Among all 154 multiple-child families, the prevalence of ophthalmic diseases was 6.5% in the oldest child, 90.8% in the second child, and 100% in the third child. Parent’s age at the time of the child’s birth was not found to be related to whether the child had an eye disease for any of the children in this study. But the parents’ highest level of educational level was associated with a lower chance of any eye disease.
Conclusion

Parity affects the prevalence of ocular disease in children. Strengthening fertility education and raising maternity security may help reduce the prevalence of eye diseases in children.