bluuebunny/crossref_metadata_2025_split huggingface dataset:
Role of rs454214 in Personality mediated Depression and Subjective Well-being
Authors: Hou Binyin , Ji Lei , Chen Zhixuan , An Lin , Zhang Naixin , Ren Decheng , Yuan Fan , Liu Liangjie , Bi Yan , Guo Zhenming , Ma Gaini , Xu Fei , Yang Fengping , Yu Shunying , Yi Zhenghui , Xu Yifeng , He Lin , Liu Chuanxin , Bai Bo , Wu Shaochang , Zhao Longyou , Cai Changqun , Yu Tao , He Guang , Shi Yi , Li Xingwang 
Published: March 2020

AbstractHappiness and depression are interlinked and both heritable, while personality, as an important predictor of them, shares the genetic basis with them. We conjecture that genetic factors of depression can affect both depressive symptoms (DS) and subjective well-being (SWB), while personality traits play important roles in mediating this process. In this study, 878 Han Chinese college freshmen and 384 Han Chinese patients with the major depressive disorder (MDD) were included. SNPs were genotyped using AGENA MassARRAY iPLEX technology and we investigated an important MDD variant rs454214. Correlation, association and mediation analysis were employed, aiming to decipher the complex relationship between SWB, DS, personality traits and the genetic variant. Association study indicated that rs454214 was not only associated with both SWB and DS (P < 0.05), but also possibly linked to MDD. Mediational analysis showed that rs454214 had no direct effect on SWB and DS, but had a significant indirect effect through personality traits, i.e., Extraversion, Neuroticism, Agreeableness and Openness to Experience or SWB, Extraversion, Neuroticism and Agreeableness for DS. This study found a shared genetic basis for happiness and depression; the causal process could be better explained if personality traits are taken as mediating factors.
Copper(II)-binding equilibria in human blood
Authors: Kirsipuu Tiina , Zadorožnaja Anna , Smirnova Julia , Friedemann Merlin , Plitz Thomas , Tõugu Vello , Palumaa Peep 
Published: March 2020

AbstractIt has been reported that Cu(II) ions in human blood are bound mainly to serum albumin (HSA), ceruloplasmin (CP), alpha-2-macroglobulin (α2M) and His, however, data for α2M are very limited and the thermodynamics and kinetics of the copper distribution are not known. We have applied a new LC-ICP MS-based approach for direct determination of Cu(II)-binding affinities of HSA, CP and α2M in the presence of competing Cu(II)-binding reference ligands including His. The ligands affected both the rate of metal release from Cu•HSA complex and the value of KD. Slow release and KD = 0.90 pM was observed with nitrilotriacetic acid (NTA), whereas His showed fast release and substantially lower KD = 34.7 fM (50 mM HEPES, 50 mM NaCl, pH 7.4), which was explained with formation of ternary His•Cu•HSA complex. High mM concentrations of EDTA were not able to elicit metal release from metallated CP at pH 7.4 and therefore it was impossible to determine the KD value for CP. In contrast to earlier inconclusive evidence, we show that α2M does not bind Cu(II) ions. In the human blood serum ~75% of Cu(II) ions are in a nonexchangeable manner bound to CP and the rest exchangeable copper is in an equilibrium between HSA (~25%) and Cu(II)-His-Xaa ternary complexes (~0.2%).
Survival stratification in childhood rhabdomyosarcoma of the extremities: a derivation and validation study
Authors: Zhu Linchao , Sun Ying , Wang Xuhui , Wang Lin , Zhang Shufeng , Meng Qinglei , Wang Xiaohui 
Published: March 2020

AbstractThe objective of this study was to estimate overall survival in children with extremity rhabdomyosarcoma (RMS). In addition, we attempted to construct a nomogram to predict the prognosis in such patients using a population-based cohort. The national Surveillance, Epidemiology, and End Results (SEER) registry was used to identify a cohort of childhood RMS patients. A total of 197 patients with RMS were ultimately included. Multivariable analysis identified age group, N classification, M classification, and treatment combinations as independent predictive factors for patient overall survival. Candidate variables such as age group, N classification, M classification, and treatment combinations were used to fit the model. For overall survival, the bootstrap-adjusted c-index was 0.76 (95% CI, 0.73–0.80) for the nomogram. Furthermore, we performed recursive partitioning analysis for risk stratification according to overall survival, and 3 prognostic subgroups were generated (low, intermediate and high risk). Finally, we evaluated multimodal treatment based on the risk stratification according to the nomogram and IRSG prognostic stratification model. With regard to the entire cohort, overall survival in patients who received surgery and radiation was superior to that in patients who received surgery or radiation (p = 0.001). Regarding RPA and IRSG prognostic stratification, we found that the differences remained significant (p < 0.05) in patients with low-intermediate risk. However, the difference disappeared in patients with high risk (p > 0.05). We performed a population-based analysis of data from the SEER registry in an effort to identify prognostic factors and develop a nomogram in children with extremity RMS. The nomogram appears to be suitable for the survival stratification of children with RMS and will help clinicians identify patients who may be at a reduced probability of survival and assist them in making treatment and surveillance decisions. More studies concerning overall survival in children with RMS are needed to confirm and update our findings.
Comparative investigation of reusable and single–use flexible endoscopes for urological interventions
Authors: Eisel Maximilian , Strittmatter Frank , Ströbl Stephan , Freymüller Christian , Pongratz Thomas , Sroka Ronald 
Published: March 2020

AbstractIn order to evaluate the technical adaptability of a type of disposable endoscope compared to reusable flexible endoscopes, in vitro and in vivo studies were conducted. A disposable digital ureteroscope (“chip on tip”) and two reusable endoscopes were investigated with respect to spatial resolution, geometric distortion in air and water the maximum. Additionally, the clinical performance of the disposable device was tested during clinical procedures (n = 20). The disposable endoscope showed an optical resolution of 6.72 lines/mm at 10 mm distance, similar to the other devices. In comparison, the disposable endoscope showed a barrel-shaped image distortion in air of −24.2%, which is in the middle range, but was best under water (−8.6%). The bendability of 297° (275 µm fiber) and 316° (empty channel, 1.5 F basket) and the maximum irrigation (1 m: 58.1 ml/min, 2 m: 91.9 ml/min) were convincing. Clinically the maneuverability was very good in (13/20), good or satisfactory in (7/20). Visibility was evaluated as very good in (11/20), just in (1/20) either satisfactory or sufficient. The consistency of visibility was not affected in (19/20). In all cases there were no adverse events. The technical examination and clinical application of the disposable endoscope are of equal quality compared to reusable devices. Disposable endoscopes can be an alternative to reusable devices, but economic aspects such as reduction of repair costs, sterilization effort and additional waste must be taken into account.
Die Rolle der gemeinschaftlichen Selbsthilfe bei der
Bewältigung einer Krebserkrankung
Authors: Kiemen Andrea , Jablotschkin Martina , Weis Joachim 
Published: March 2020

Zusammenfassung
Hintergrund In Selbsthilfegruppen erhalten Betroffene
krankheitsspezifische Informationen und psychosoziale Unterstützung aus
der Erfahrung Gleichbetroffener. Wichtige Aspekte von Patientenkompetenz wie die
Entwicklung einer aktiven ressourcengestützten Krankheitsverarbeitung
können gefördert werden. Mitglieder von Selbsthilfegruppen
berichten von der Erfahrung, selbstwirksam Einfluss auf das Erleben der
Erkrankung nehmen zu können.
Forschungsergebnisse Bisherige Erkenntnisse aus internationalen
Studienergebnissen zur Selbsthilfeforschung zeigen, dass das Potenzial der
Selbsthilfe darin besteht, einen großen Einfluss auf die
Krankheitsbewältigung und die damit verbundene Lebensqualität
von Betroffenen haben zu können. Durch ihre Teilnahme an
Selbsthilfegruppen erfahren Patienten emotionale Entlastung, eine
Unterstützung in der Entscheidung für oder gegen eine
medizinische Therapie und erlangen eine bessere Akzeptanz ihrer Erkrankung.
Darüber hinaus wird die Beziehung zu Partnern oder
Familienangehörigen entlastet und verbessert.
Ausblick Strukturelle Aspekte wie die Evaluation von
Optimierungsprozessen zur Integration der Selbsthilfe in das Versorgungssystem
und die Qualitätsverbesserung von Selbsthilfeaktivitäten durch
gezielte Schulung und Fortbildung der Selbsthilfevertreter sind wichtige
Forschungsthemen.
Schlussfolgerungen Die systematische Untersuchung von Prozess- und
Wirkfaktoren der individualisierten sowie gruppenbasierten Selbsthilfe entlang
patientenorientierter Konzepte wie Patientenkompetenz können dazu
beitragen, die Versorgung von Patientinnen und Patienten mit Krebserkrankung zu
verbessern.
Scope of Management of Noncommunicable Diseases in India through Ayushman Bharat
Authors: Verma Madhur 
Published: March 2020

AbstractIndia is a rapidly developing country, and has been going through a major epidemiological transition over the past 25 years. As per the India State-Level Disease Burden Initiative, every state of India now has a higher burden from noncommunicable diseases (NCDs) and injuries than from infectious diseases. Inspired by Sustainable Development Goal vision, mitigating the effect of NCDs will demand a comprehensive approach based on preventive, promotive, and curative cum rehabilitative services. Apart from the community-based approach, another key announcement made in the union budget of India, 2018–19, was the Ayushman Bharat Pradhan Mantri Jan Arogya Yojana (ABY) that intend to transform the primary, secondary, and tertiary health care system in India. In this article, we intend to analyze the scope of services offered through ABY at different levels of public health care, and possible constraints in realizing the goal of universal health coverage in terms of NCDs.
Metachronic Breast and Cerebellar Neoplasm in a Young Patient
Authors: Nascimento Jéssika de Oliveira , Haro Lara Caroline Anastacio , Sá Rafael da Silva , Storch Rebeca Espelho 
Published: March 2020

AbstractSeveral factors trigger the development of genetic mutations that are responsible for causing a neoplasm. Medulloblastoma is a malignant and invasive cerebellar neoplasm, that affects children and young adults. Mucinous carcinoma is a special type of breast cancer. Being a special atypical subtype of invasive carcinoma, it most frequently affects women of advanced age and represents 1 to 7% of all breast cancers. The reported case aims to show the rarity of the occurrence of desmoplastic medulloblastoma and mammary mucinous carcinoma in a young patient in a short period of time, in different sites, without direct anatomical attachment and without occurrence of metastasis. Initially, this patient had a desmoplastic medulloblastoma and was treated with lumpectomy and radiotherapy. After 13 months, the patient was diagnosed with a mucinous breast carcinoma, underwent mastectomy, adjuvant chemotherapy and is currently undergoing endocrinotherapy. We conclude, based on the metachronous characteristic of the neoplasia and clinical characteristics, that the patient is likely to have Li-Fraumeni syndrome, an autosomal dominant disease with mutation of the TP53 gene, which is the the main involved. Because the patient does not present all the characteristics of the phenotype of the syndrome, she can thus be classified as having Li-Fraumeni variant or Li-Fraumeni-like syndrome.
Factors Associated with Inadequate Birth Intervals in the BRISA Birth Cohort, Brazil
Authors: Barbosa Raphael , Alves Maria Teresa Seabra Soares Britto , Nathasje Ian , Chagas Deysianne , Simões Vanda Ferreira , Silva Leonardo 
Published: March 2020

Abstract
Objective To determine the prevalence of inadequate birth interval and its associated factors in the BRISA study.
Methods Cross-sectional study using data from the BRISA cohort. Birth interval was categorized into “adequate” (≥ 2 years or < 5 years between births), “short interval” (< 2 years) and “long interval” (≥ 5 years). The analysis of the factors associated with short and long birth intervals used multinomial logistic regression.
Results The prevalence of adequate birth intervals was 48.3%, of long intervals, 34.6%, and of short intervals, 17.1%. Skin color, age, education level, economic status, type of delivery, number of prenatal visits, parity, blood pressure, diabetes, and anemia (p-value was < 0.2 in the univariate analysis) proceeded to the final model. The variable ≥ 3 births (odds ratio [OR] = 1.29; confidence interval [CI]: 1.01–1.65) was associated with short intervals. Age < 20 years old (OR = 0.48; CI: 0.02–0.12) or ≥ 35 years old (OR = 2.43; CI: 1.82–3.25), ≥ 6 prenatal visits (OR = 0.58; CI: 0.47–0.72), ≥ 3 births (OR = 0.59; CI: 0.49–0.73), and gestational diabetes (OR = 0.38; CI: 0.20–0.75) were associated with long intervals.
Conclusion Older mothers were more likely to have long birth intervals, and higher parity increases the chances of short birth intervals. Furthermore, gestational diabetes and adequate prenatal care presented higher chances of having adequate birth intervals, indicating that health assistance during pregnancy is important to encourage an adequate interval between gestations.
Genital Injuries: Are They Telling us Something about Sexual Violence?
Authors: Orellana-Campos Carolina 
Published: March 2020

AbstractGenital injury has a forensic relevance after a sexual assault and it has been discussed and investigated among professionals who work in this field. To analyze the studies published in the last decades, the present review examines different factors that may influence this finding, first clarifying terms of the forensic field, such as the peculiarity of the legal medical examination, and the distinction of the terms “legal” and “anatomical” vagina. Finally, it analyses if it is possible that the existence of these injuries in victims explain the lack of consent in sexual contact, and to clarify the meaning of the absence of injuries.
Influence of Interlocking Horizontal Mattress Epitendinous Suture Placement on Tendinous Biomechanical Properties in a Canine Common Calcaneal Laceration Model
Authors: Cocca Christina J. , Duffy Daniel J. , Kersh Mariana E. , Moore George E. 
Published: March 2020

Abstract
Objective This article evaluates the effect of an interlocking horizontal mattress epitendinous suture (IHMES) in addition to a three-loop pulley (3LP) core suture for canine tendon repair.
Study Design Twenty-eight cadaveric common calcaneal tendons were randomized, sharply transected and repaired with either a 3LP or 3LP + IHMES. Tensile loads required to create a 1- and 3-mm gap, yield, peak and failure loads, and mode of failure were analysed. Significance was set at p < 0.05.
Results Mean ± standard deviation yield and failure force for 3LP + IHMES was 178.0 ± 45.3 N and 242.1 ± 47.8 N, respectively, which was significantly greater compared with 3LP alone, 97.9 ± 36.2 N and 119.3 ± 35.6 N (p < 0.0001). Occurrence of 3-mm gap formation was significantly less in the 3LP + IHMES group (p < 0.013). Mode of failure was significantly different between the groups (p < 0.001) with 3LP + IMHES patterns failing by suture breakage (13/14) compared with suture pull-through in the 3LP (11/14).
Conclusion Addition of an epitendinous suture pattern significantly reduced gap formation between tendon ends and significantly increased loads at yield (1.8 × ), peak (2.0 × ) and failure (2.0 × ) force of repairs. Use of an epitendinous suture should be considered to significantly increase biomechanical strength of repairs; however, further in vivo testing is necessary to evaluate its effect on tendinous blood supply.
Recent Progress in Hydrogen-Bonded π-Conjugated Systems Displaying J-Type Aggregates
Authors: Ávila-Rovelo Nelson Ricardo , Ruiz-Carretero Amparo 
Published: March 2020

Supramolecular approaches are of great interest in the design of functional materials. The types of aggregates arising from different noncovalent interactions endow materials with intriguing properties. In this sense, J-type aggregates are very attractive due to their unique optical properties and capacity to transport excitons. These features make them great candidates in the design of materials for organic electronic devices. Furthermore, the incorporation of additional hydrogen-bonding functionalities provides J-aggregates with superior directionality and connection among the different π-conjugated cores. The control over the formation of H-bonds to achieve functional aggregates is therefore a promising strategy towards controlled structures with specific functions.This review outlines the most relevant and recent works of π-conjugated systems exhibiting J-type aggregates resulting from hydrogen-bonding interactions. Different types of hydrogen-bonding functionalities will be discussed together with their roles in the aggregate properties, their impact in the optoelectronic properties, the self-assembly mechanisms, and their applications in organic electronics.
Metaphor and the popularization of contested technologies
Authors: von Wülfingen Bettina Bock 
Published: March 2020

Abstract
This contribution analyzes metaphors in expert bioscientific texts on reproductive technologies from cloning
to pre-implantation genetic diagnosis in German print media during the time when, according to some German
journalists, restrictive attitudes seemed ripe for change. The study uses systematic metaphor analysis to investigate
the functional content of metaphors for those producing a text. It shows how conventional metaphors
contribute to the popularization of science, as they bring new reproductive technologies into the realm of our
everyday experience. For scientists, this work shows the fine line between explanatory use of metaphors and
distortions which can harm the reputation of science. It may foster a nonscientist’s ability to interpret metaphors in
the production of hope in the promise of new technologies.
Subtle discriminatory political discourse on immigration
Authors: Rubio-Carbonero Gema 
Published: March 2020

Abstract
Overt constructions of discrimination in political discourse towards immigrants are easy to detect and have been
traditionally associated with far-right parties. However, mainstream political discourse on immigration delivered by so-called
center or center-left parties has transformed into more subtle forms of discursive discrimination, which might not be obvious and
need a closer analysis in order to be spotted in discourse. Overt discriminatory discourse has been studied by disciplines, such
as political sociology, social psychology and Critical Discourse Studies, but subtle discriminatory constructions have been rather
neglected. By combining these three disciplines, we propose here a multidisciplinary and multitheorical framework to
systematically analyze subtle discriminatory political discourse on immigration. It aims at contributing to the development of a
methodology for a socio-political analysis that allows to detect subtle discriminatory political discourse on immigration. Such
framework is composed by four strategies with different degrees of subtleness: highlighting, diminishing, homogenizing and
normalizing.
Effect of concentric vs. eccentric variant of lower limbs plyometric training on biomechanical and biochemical parameters
Authors: Staniszewski Michał , Hammoud Naji , Zybko Przemysław , Witek Katarzyna , Tkaczyk Joanna , Urbanik Czesław 
Published: March 2020

AbstractThe study aimed to compare the effectiveness of two variants of 4 weeks plyometric training involving jumping up (dominance of concentric muscle work, UP) and jumping down (dominance of eccentric muscle work, DOWN) the stairs. Twenty‐six young men were divided into two groups: UP (n = 13) and DOWN (n = 13). Control measurements of the muscle torques, vertical jumps, and creatine kinase (CK) activity were performed every week. The training resulted in significant changes (p < 0.001) in the muscle torques of the hip extensors (Δ ≈ 20% in both groups) and plantar flexors (ΔUP = 25%, ΔDOWN = 33%), but in the muscle torques of the knee extensors a significant (p < 0.001) increase occurred only in the group jumping down the stairs (ΔDOWN ≈ 15%). Furthermore, a significant increase (p < 0.001) of the height of the vertical jump was noted in the study only in the group jumping down (ΔDOWN = 8%). Training with the dominance of eccentric work caused greater weekly changes in the activity of creatine kinase in the plasma. The study confirmed that the exercise involving jumping on the stairs is viable in plyometric training, as it is an effective means of training the strength of the muscles of the lower limbs and vertical jump height, with a greater share assigned to jumping down consecutive steps.
Heterogeneity of the nucleic acid repertoire of plasma extracellular vesicles demonstrated using high‐sensitivity fluorescence‐activated sorting
Authors: Kondratov Kirill , Nikitin Yuri , Fedorov Anton , Kostareva Anna , Mikhailovskii Vladimir , Isakov Dmitry , Ivanov Andrey , Golovkin Alexey 
Published: March 2020

ABSTRACTThe aim of this study was to investigate cell source‐dependent nucleic acids repertoire of diverse subpopulations of plasma extracellular vesicles (EVs). Blood plasma from nine healthy volunteers was used for the analysis. Samples of EVs were obtained by differential centrifugation of plasma. The application of high‐sensitivity fluorescence‐activated vesicles sorting (hs‐FAVS) using fluorophore‐conjugated anti‐CD41‐FITC (Fluorescein isothiocyanate) and anti‐CD235a‐PE antibodies allowed the isolation of three subpopulations of EVs, namely CD41+ CD235a‐, CD41‐CD235a+ and CD41‐CD235a dim. The high purity (>97%) of the sorted subpopulations was verified by high‐sensitivity flow cytometry. Presence of nanosized objects in sorted samples was confirmed by combination of low‐voltage scanning electron microscopy and dynamic light scattering. The amount of material in sorted samples was enough to perform Quantitative polymerase chain reaction (qPCR)‐based nucleic acid quantification. The most prominent differences in the nucleic acid repertoire were noted between CD41+ CD235‐ vs. CD41‐CD235a+ vesicles: the former contained significantly (p = 0.004) higher amount of mitochondrial DNA, and platelet enriched miR‐21‐5p (4‐fold), miR‐223‐3p (38‐fold) and miR‐199a‐3p (187‐fold), but lower amount of erythrocyte enriched miR‐451a (90‐fold). CD41‐CD235a+ and CD41‐CD235a dim vesicles differed in levels of miR‐451a (p = 0.016) and miR‐21‐5p (p = 0.031). Nuclear DNA was below the limit of detection in all EV subpopulations. The hs‐FCM‐based determination of the number of sorted EVs allowed the calculation of per single‐event miRNA concentrations. It was demonstrated that the most abundant marker in CD41+ CD235a‐ subpopulation was miR‐223‐3p, reaching 38.2 molecules per event. In the CD41‐CD235+ subpopulation, the most abundant marker was miR‐451a, reaching 24.7 molecules per event. Taken together, our findings indicate that erythrocyte‐ and platelet‐derived EVs carry different repertoires of nucleic acids, which were similar to the composition of their cellular sources.