bluuebunny/crossref_metadata_2025_split huggingface dataset:
Establishment of long-term serum-free culture for lacrimal gland stem cells aiming at lacrimal gland repair
Authors: Xiao Sa , Zhang Yan 
Published: January 2020

AbstractBackgroundAqueous-deficient dry eye disease (ADDED) resulting from dysfunction of the lacrimal gland (LG) is currently incurable. Although LG stem/progenitor cell-based therapy is considered to be a promising strategy for ADDED patients, the lack of a reliable serum-free culture method to obtain enough lacrimal gland stem cells (LGSCs) and the basic standard of LGSC transplantation are obstacles for further research.MethodsAdult mouse LGSCs were cultured in Matrigel-based 3D culture under serum-free culture condition, which contained EGF, FGF10, Wnt3A, and Y-27632. LGSCs were continuously passaged over 40 times every 7 days, and the morphology and cell numbers were recorded. LGSCs were induced to differentiate to ductal cells by reducing Matrigel rigidity, while fetal bovine serum was used for the induction of acinar cells. RT-PCR or qRT-PCR analysis, RNA-sequence analysis, H&E staining, and immunofluorescence were used for characterization and examining the differentiation of LGSCs. LGSCs were allotransplanted into diseased LGs to examine the ability of repairing the damage. The condition of eye orbits was recorded using a camera, the tear production was measured using phenol red-impregnated cotton threads, and the engraftments of LGSCs were examined by immunohistochemistry.ResultsWe established an efficient 3D serum-free culture for adult mouse LGSCs, in which LGSCs could be continuously passaged for long-term expansion. LGSCs cultured from both the healthy and ADDED mouse LGs expressed stem/progenitor cell markers Krt14, Krt5, P63, and nestin, had the potential to differentiate into acinar or ductal-like cells in vitro and could engraft into diseased LGs and relieve symptoms of ADDED after orthotopic injection of LGSCs.ConclusionWe successfully established an efficient serum-free culture for adult mouse LGSCs aiming at LG repair for the first time. Our approach provides an excellent theoretical and technical reference for future clinical research for ADDED stem cell therapy.
Mol-CycleGAN: a generative model for molecular optimization
Authors: Maziarka Łukasz , Pocha Agnieszka , Kaczmarczyk Jan , Rataj Krzysztof , Danel Tomasz , Warchoł Michał 
Published: January 2020

AbstractDesigning a molecule with desired properties is one of the biggest challenges in drug development, as it requires optimization of chemical compound structures with respect to many complex properties. To improve the compound design process, we introduce Mol-CycleGAN—a CycleGAN-based model that generates optimized compounds with high structural similarity to the original ones. Namely, given a molecule our model generates a structurally similar one with an optimized value of the considered property. We evaluate the performance of the model on selected optimization objectives related to structural properties (presence of halogen groups, number of aromatic rings) and to a physicochemical property (penalized logP). In the task of optimization of penalized logP of drug-like molecules our model significantly outperforms previous results.
LOX family and ZFPM2 as novel diagnostic biomarkers for malignant pleural mesothelioma
Authors: Kim Min-Kyu , Kim Hyun-won , Jang Mirae , Oh Sung Soo , Yong Suk-Joong , Jeong Yangsik , Jung Soon-Hee , Choi Jong-Whan 
Published: January 2020

AbstractBackgroundMalignant pleural mesothelioma (MPM) is a rare and aggressive cancer that develops in the pleural and outer layer of tissues surrounding the lungs. MPM is primarily caused by occupational exposure to asbestos and results in a poor prognosis. Effective therapeutics as well as early diagnostics for the MPM are still lacking. To identify potential diagnostic biomarkers for MPM, we performed bioinformatics analysis of public database.MethodsUtilizing databases from Cancer Cell Line Encyclopedia (CCLE) and Gene Expression Omnibus (GEO), we identified several potential candidates that could act as MPM biomarkers. We carried out additional molecular analyses of these potential markers using MPM patient tissue samples via quantitative polymerase chain reaction.ResultsWe identified Lysyl oxidase (LOX), Lysyl oxidase homologs 1&2 (LOXL1& LOXL2) Zinc Finger Protein, FOG Family Member 2 (ZFPM2) as potential diagnostic biomarkers for MPM. In this study, we found that the LOX family and ZFPM2 showed comparable diagnostic ability to Fibulin-3 or mesothelin (MSLN) and would be better potential biomarkers than Sulfatase 1 (SULF1), Thrombospondin 2 (THBS2) and Cadherin 11 (CDH11).ConclusionsLOX family and ZPFM2 were identified as novel MPM diagnostic biomarkers which could strengthen MPM clinical diagnostic capabilities.
Correction to: Comprehensive gene expression meta-analysis identifies signature genes that distinguish microglia from peripheral monocytes/macrophages in health and glioma
Authors: Haage Verena , Semtner Marcus , Vidal Ramon Oliveira , Hernandez Daniel Perez , Pong Winnie W. , Chen Zhihong , Hambardzumyan Dolores , Magrini Vincent , Ly Amy , Walker Jason , Mardis Elaine , Mertins Philipp , Sauer Sascha , Kettenmann Helmut , Gutmann David H. 
Published: January 2020

The original publication of this article [1] contained 3 minor errors in Figs. 1, 3 and 5. In this correction article the updated figures are published. The figure captions describe the updated information in these figures.
Innate signaling within the central nervous system recruits protective neutrophils
Authors: Khorooshi Reza , Marczynska Joanna , Dieu Ruthe Storgaard , Wais Vian , Hansen Christian Rønn , Kavan Stephanie , Thomassen Mads , Burton Mark , Kruse Torben , Webster Gill A. , Owens Trevor 
Published: January 2020

AbstractThere is great interest in understanding how the central nervous system (CNS) communicates with the immune system for recruitment of protective responses. Infiltrating phagocytic monocytes and granulocytes are implicated in neuroinflammation in multiple sclerosis and its animal model experimental autoimmune encephalomyelitis (EAE). To investigate how CNS endogenous signals can be harnessed to promote anti-inflammatory programs, we have used a particulate Toll-like receptor 9 and nucleotide-oligomerization domain 2 bispecific innate ligand (MIS416), to address whether its phagocytosis within the CNS recruits protective myeloid cells. We find that MIS416 injected intrathecally into the cerebrospinal fluid via the cisterna magna induced a local chemokine response that recruited blood-derived monocytes and neutrophils to the CNS. These cells phagocytosed MIS416. The increase in EAE severity normally seen from time of onset did not occur in mice receiving MIS416. This suppression of disease symptoms was dependent on expression of the type I interferon receptor (IFNAR). Transfer of intrathecal MIS416-induced neutrophils suppressed EAE in recipient mice, while monocytes did not transfer protection. MIS416-induced neutrophils showed increased IL-10 expression that was IFNAR1-driven. In contrast to intrathecal administration, intravenous administration of MIS416 led to monocyte but not neutrophil infiltration to the CNS. We thus identify a CNS-intrinsic and -specific phagocytosis-induced recruitment of anti-inflammatory neutrophils that contribute to CNS homeostasis and may have therapeutic potential.
Health economic evaluations of sepsis interventions in critically ill adult patients: a systematic review
Authors: Higgins Alisa M. , Brooker Joanne E. , Mackie Michael , Cooper D. Jamie , Harris Anthony H. 
Published: January 2020

Abstract
Background
Sepsis is a global health priority. Interventions to reduce the burden of sepsis need to be both effective and cost-effective. We performed a systematic review of the literature on health economic evaluations of sepsis treatments in critically ill adult patients and summarised the evidence for cost-effectiveness.

Methods
We systematically searched MEDLINE, Embase, and the Cochrane Library using thesaurus (e.g. MeSH) and free-text terms related to sepsis and economic evaluations. We included all articles that reported, in any language, an economic evaluation of an intervention for the management of sepsis in critically ill adult patients. Data extracted included study details, intervention details, economic evaluation methodology, and outcomes. Included studies were appraised for reporting quality using the Consolidated Health Economic Evaluation Reporting Standards (CHEERS) checklist.

Results
We identified 50 records representing 46 economic evaluations for a variety of interventions including antibiotics (n = 5), fluid therapy (n = 2), early goal-directed therapy and other resuscitation protocols (n = 8), immunoglobulins (n = 2), and interventions no longer in clinical use such as monoclonal antibodies (n = 7) and drotrecogin alfa (n = 13). Twelve (26%) evaluations were of excellent reporting quality. Incremental cost-effectiveness ratios (ICERs) ranged from dominant (lower costs and higher effectiveness) for early goal-directed therapy, albumin, and a multifaceted sepsis education program to dominated (higher costs and lower effectiveness) for polymerase chain reaction assays (LightCycler SeptiFast testing MGRADE®, SepsiTest™, and IRIDICA BAC BSI assay). ICERs varied widely across evaluations, particularly in subgroup analyses.

Conclusions
There is wide variation in the cost-effectiveness of sepsis interventions. There remain important gaps in the literature, with no economic evaluations identified for several interventions routinely used in sepsis. Given the high economic and social burden of sepsis, high-quality economic evaluations are needed to increase our understanding of the cost-effectiveness of these interventions in routine clinical practice and to inform decision makers.

Trial registration
PROSPERO CRD42018095980
Hypomagnesaemia induced recurrent cerebellar ataxia: an interesting case with successful management
Authors: Kumar Singh Saraj , Khushbu Goel , Dev Mukherji Joy 
Published: January 2020

Abstract
Purpose
Severe Hypomagnesaemia is a rare biochemical findings utilized for identifying the etiology of cerebellar ataxia. It requires a high degree of suspicion to diagnose. MRI findings are often nonspecific.

Methods
The author presents a case of 38 yrs. old male patient presented with vomiting, gait imabalance and nystagmus. Biochemical investigations lead to severe hypomagnesaemia. Also MRI findings were matched suggesting of hyperintesity in left cerebellar hemisphere.

Results
Patient was treated with magnesium infusion which leads to recovery of patient. Again the same symptomology was repeated after 3 months and disappearance after same treatment. Offending cause was diagnosed and proton pump inhibitors stopped.

Conclusion
Severe Hypomagnesaemia is a rare but treatable cause if diagnosed at right time. It requires a high degree of suspicion to diagnose it. Measurement of serum magnesium levels should always be kept in back of mind if definite management of cerebellar symptoms has to be done.
Torsion of vermiform appendix: case report and review of the literature
Authors: Endo Kosuke , Sato Masahito , Saga Kenichi , Higashio Atsushi , Yuba Yoshiaki , Morotomi Yoshiki 
Published: January 2020

AbstractBackgroundTorsion of the vermiform appendix is a rare disease with symptoms very similar to those of acute appendicitis. We herein report a case of torsion of the vermiform appendix diagnosed by intraoperative findings.Case presentationA 4-year-old boy presented to our hospital because of abdominal pain and vomiting. Laboratory data revealed a C-reactive protein level of 0.08 mg/dL and white blood cell count of 19,300/μL (neutrophils, 88.9%). Abdominal ultrasound showed a target sign-like finding in the ileocecal region. A computed tomography scan showed swelling of the appendix. We performed an emergency operation under suspicion of acute appendicitis. Laparoscopic examination showed that the appendix had twisted 720° in the clockwise direction. Appendectomy was performed, and the postoperative course was uneventful.ConclusionsAlthough torsion of the vermiform appendix is a very rare disease and difficult to differentiate from appendicitis, an inappropriate treatment plan could lead to necrosis and perforation of the appendix. It is important to consider this disease as a differential diagnosis in patients with right lower abdominal pain.
On the accuracy of macroscopic equations for linearized rarefied gas flows
Authors: Wu Lei , Gu Xiao-Jun 
Published: January 2020

AbstractMany macroscopic equations are proposed to describe the rarefied gas dynamics beyond the Navier-Stokes level, either from the mesoscopic Boltzmann equation or some physical arguments, including (i) Burnett, Woods, super-Burnett, augmented Burnett equations derived from the Chapman-Enskog expansion of the Boltzmann equation, (ii) Grad 13, regularized 13/26 moment equations, rational extended thermodynamics equations, and generalized hydrodynamic equations, where the velocity distribution function is expressed in terms of low-order moments and Hermite polynomials, and (iii) bi-velocity equations and “thermo-mechanically consistent" Burnett equations based on the argument of “volume diffusion”. This paper is dedicated to assess the accuracy of these macroscopic equations. We first consider the Rayleigh-Brillouin scattering, where light is scattered by the density fluctuation in gas. In this specific problem macroscopic equations can be linearized and solutions can always be obtained, no matter whether they are stable or not. Moreover, the accuracy assessment is not contaminated by the gas-wall boundary condition in this periodic problem. Rayleigh-Brillouin spectra of the scattered light are calculated by solving the linearized macroscopic equations and compared to those from the linearized Boltzmann equation. We find that (i) the accuracy of Chapman-Enskog expansion does not always increase with the order of expansion, (ii) for the moment method, the more moments are included, the more accurate the results are, and (iii) macroscopic equations based on “volume diffusion" do not work well even when the Knudsen number is very small. Therefore, among about a dozen tested equations, the regularized 26 moment equations are the most accurate. However, for moderate and highly rarefied gas flows, huge number of moments should be included, as the convergence to true solutions is rather slow. The same conclusion is drawn from the problem of sound propagation between the transducer and receiver. This slow convergence of moment equations is due to the incapability of Hermite polynomials in the capturing of large discontinuities and rapid variations of the velocity distribution function. This study sheds some light on how to choose/develop macroscopic equations for rarefied gas dynamics.
Two-incision laparoscopic cholecystectomy performed via the “marionette” technique versus conventional laparoscopic cholecystectomy in pediatrics
Authors: Shehata Mohamed A. , Ebeid Abdelmotaleb E. , El Attar Ashraf A. 
Published: January 2020

Abstract
Background
Laparoscopic cholecystectomy (LC) has considered the gold standard for the treatment of symptomatic gallstones. The “marionette” technique is a surgical technique for performing a safe, two-incision laparoscopic cholecystectomy without the use of an additional port or any new access devices. This study aimed to compare between the cost and outcome of two incisions LC performed via the marionette technique and the standard LC in pediatrics.

Results
All 32 children with symptomatic cholecystolithiasis were discharged from the hospital in the first postoperative day with no statistically significant difference within days to return to normal work (p = 0.607). The operative time in the group (A) which included 16 children who underwent 2 incisions marionette style LC technique was non-significantly longer time (p = 0.184) than the group (B) which included 16 children who underwent conventional 4 incisions LC technique. Visual analog scores for pain showed a significant difference between both groups at 6 h and 12 h postoperative (p = 0.000 and 0.003, respectively). Overall patient in marionette group was very satisfied by fewer incision and better cosmesis.

Conclusion
Marionette technique performed by an experienced surgeon among pediatric shows a statistically significant cost benefit while maintaining good operative time, less postoperative pain, and more patient’s satisfaction and provides excellent aesthetic results.
Inversion of differences in frequency components of azimuthal seismic data for indicators of oil-bearing fractured reservoirs based on an attenuative cracked model
Authors: Chen Huaizhen , Li Junxiao , Innanen Kristopher A. 
Published: January 2020

Based on a model of attenuative cracked rock, we have derived a simplified and frequency-dependent stiffness matrix associated with (1) a rock volume containing aligned and partially saturated cracks and (2) a new indicator of oil-bearing fractured reservoirs, which is related to pressure relaxation in cracked rocks and influenced by fluid viscosity and saturation. Starting from the mathematical form of a perturbation in this stiffness matrix across a reflecting interface separating two attenuative cracked media, we set up a linearized P-wave to P-wave reflection coefficient as an azimuthally and frequency-dependent function of dry rock elastic properties, dry fracture weaknesses, and the new indicator. By varying this reflection coefficient with azimuthal angle, we derive a further expression referred to as the quasidifference in elastic impedance, or [Formula: see text], which is primarily affected by the dry fracture weaknesses and the new indicator. An inversion approach is established to use differences in frequency components of seismic amplitudes to estimate these weaknesses and the indicator based on the derived [Formula: see text]. In synthetic inversion tests, we determine that the approach produces interpretable parameter estimates in the presence of data with a moderate signal-to-noise ratio (S/N). Testing on a real data set suggests that reliable fracture weakness and indicator are generated by the approach; fractured and oil-bearing reservoirs are identified through a combination of the dry fracture weakness and the new indicator.
Exploring psychological symptoms and associated factors in patients receiving medication-assisted treatment for opioid-use disorder
Authors: Rosic Tea , Worster Andrew , Thabane Lehana , Marsh David C. , Samaan Zainab 
Published: January 2020

Background
Patients receiving treatment for opioid-use disorder (OUD) may experience psychological symptoms without meeting full criteria for psychiatric disorders. The impact of these symptoms on treatment outcomes is unclear.

Aims
To determine the prevalence of psychological symptoms in a cohort of individuals receiving medication-assisted treatment for OUD and explore their association with patient characteristics and outcomes in treatment.

Method
Data were collected from 2788 participants receiving ongoing treatment for OUD recruited in two Canadian prospective cohort studies. The Maudsley Addiction Profile psychological symptoms subscale was administered to all participants via face-to-face interviews. A subset of participants (n = 666) also received assessment for psychiatric disorders with the Mini International Neuropsychiatric Interview. We used linear regression analysis to explore factors associated with psychological symptom score.

Results
The mean psychological symptom score was 12.6/40 (s.d. = 9.2). Participants with psychiatric comorbidity had higher scores than those without (mean 16.8 v. 8.6, P<0.001) and 31% of those with psychiatric comorbidity reported suicidal ideation. Higher psychological symptom score was associated with female gender (B = 1.59, 95% CI 0.92–2.25, P<0.001), antidepressant prescription (B = 4.35, 95% CI 3.61–5.09, P<0.001), percentage of opioid-positive urine screens (B = 0.02, 95% CI 0.01–0.03, P<0.001), and use of non-opioid substances (B = 1.92, 95% CI 0.89–2.95, P<0.001). Marriage and employment were associated with lower psychological symptoms.

Conclusions
Psychological symptoms are associated with treatment outcomes in this population and the prevalence of suicidal ideation is an area of concern. Our findings highlight the ongoing need to optimise integrated mental health and addictions services for patients with OUD.
Circulating Unmethylated Insulin DNA As a Biomarker of Human Beta Cell Death: A Multi-laboratory Assay Comparison
Authors: Speake Cate , Ylescupidez Alyssa , Neiman Daniel , Shemer Ruth , Glaser Benjamin , Tersey Sarah A , Usmani-Brown Sahar , Clark Pamela , Wilhelm Joshua J , Bellin Melena D , Herold Kevan C , Mirmira Raghavendra G , Dor Yuval , Evans-Molina Carmella 
Published: January 2020

Abstract

Context
There is an unmet need for biomarkers of pancreatic beta-cell death to improve early diagnosis of type 1 diabetes, enroll subjects into clinical trials, and assess treatment response. To address this need, several groups developed assays measuring insulin deoxyribonucleic acid (DNA) with unmethylated CpG sites in cell-free DNA. Unmethylated insulin DNA should be derived predominantly from beta-cells and indicate ongoing beta-cell death.

Objective
To assess the performance of three unmethylated insulin DNA assays.

Design and Participants
Plasma or serum samples from 13 subjects undergoing total pancreatectomy and islet autotransplantation were coded and provided to investigators to measure unmethylated insulin DNA. Samples included a negative control taken post-pancreatectomy but pretransplant, and a positive control taken immediately following islet infusion. We assessed technical reproducibility, linearity, and persistence of detection of unmethylated insulin DNA for each assay.

Results
All assays discriminated between the negative sample and samples taken directly from the islet transplant bag; 2 of 3 discriminated negative samples from those taken immediately after islet infusion. When high levels of unmethylated insulin DNA were present, technical reproducibility was generally good for all assays.

Conclusions
The measurement of beta cell cell-free DNA, including insulin, is a promising approach, warranting further testing and development in those with or at-risk for type 1 diabetes, as well as in other settings where understanding the frequency or kinetics of beta cell death could be useful.