bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Effect of Dialysate Dwell on Gastric Emptying Time in Patients on Continuous Ambulatory Peritoneal Dialysis
Authors: Kim Dae Joong , Kang Woo-Heon , Kim Hae Young , Lee Bang Hoon , Kim Bum , Lee Sung Ku , Oh Dong Jin , Huh Wooseong , Oh Ha-Young 
Published: February 2020

Methods and Patients We evaluated gastric emptying time (GET) with a technetium (Tc) 99m-sulfur colloid gastric emptying scan in 11 patients on continuous ambulatory peritoneal dialysis (CAPO) (6 males, 5 females) and in 14 controls. We investigated the effect of dialysate dwell on GET by studying the subjects twice: once without dialysate in the abdomen (drained) and once with 2 L of dialysate in the abdomen (full). We also investigated the relationship between body surface area (BSA) and delayed gastric emptying. Results (1) The mean gastric emptying rate in 120 minutes in patients on CAPO when drained (67.8% ± 13.4%) was not different from that in controls (65.4% ± 8.6%). (2) The mean gastric emptying rate in 120 minutes in patients on CAPO when full was significantly slower than that when drained (55.6% ± 14.6% versus 67.8% ± 13.4%, p < 0.05). In four of the 11 patients (36.4%), gastric emptying was extremely delayed from normal to abnormal range when full. (3) The BSA of patients who had extremely delayed GET from normal to abnormal range was smaller than that of patients who had minimal delayed or unchanged GET when full (1.5 ± 0.11 m2 versus 1.74 ± 0.22 m2). Conclusion This study showed that patients on CAPO had normal gastric emptying when drained, and that gastric emptying was delayed by dialysate dwell, especially in patients who has less than 1.5 m2 of body surface area. Therefore, we suggest that, based on adequacy, intermittent nocturnal peritoneal dialysis or a small volume of dialysate be considered for patients with small body surface area.
National Comparisons: Optimal Peritoneal Dialysis Outcomes among Japanese Patients
Authors: Kawaguchi Yoshindo 
Published: February 2020

This paper describes the current status of chronic dialysis in Japan and the guidelines used to initiate dialysis (scoring system), and reports the outcome of continuous ambulatory peritoneal dialysis (CAPD), focusing upon our center's experience. Fifty percent of CAPD technique survival was 6.9 ± 1.3 years among those patients classified as “positive selection.” The major causes of withdrawal from CAPD were ultrafiltration failure, the patients’ inability to continue on CAPD by themselves, and peritonitis. The clinical issues that most concern nephrologists in CAPD management are prevention and management of ultrafiltration failure, prevention/therapeutic intervention in encapsulating peritoneal sclerosis, catheter-related infections, and prevention of underdialysis.
Efficacy of Integrative Body-Mind-Spirit Group Intervention for Parents of Children With Eczema: A Randomized, Wait-List Controlled Clinical Trial
Authors: Fung Yat-Lui , Leung Hiu-Tin , Chan Celia H. Y. , Lau Bobo H. P. , Chan Cecilia L. W. 
Published: February 2020

Objectives: This randomized controlled trial evaluated the effects of a psychosocial intervention developed based on the Integrative Body-Mind-Spirit (IBMS) model that aimed to enhance the well-being of parents of children with eczema. Methods: Ninety-one families were randomly allocated to either the six-session intervention group ( n = 48) or the wait-list control group ( n = 43) and completed the randomized trial. For both groups, a range of psychosocial outcome measures were taken before the intervention (T0), postintervention (T1), and 6 weeks after the intervention (T2). Results: Relative to the control group, the intervention group was significantly improved over time in their levels of perceived stress, depression, and a number of holistic well-being measures, including nonattachment, afflictive ideation, and general vitality. Discussion: The results provided empirical support for an IBMS-informed psychosocial intervention in reducing stress and depression and enhancing well-being among parents of children with eczema.
The Concept of Family’s Harmony in Multiple Cultural Settings, What About the Family Harmony With Autism Children in Indonesia? A Literature Study
Authors: Fauziah Nailul 
Published: February 2020

Purposes: This literature review aims to systematically examine antecedents or factors that influence the outcomes of and to describe the dynamics of family harmony as a state. Therefore, the research questions behind this review are as follows: What factors are known to influence or predict the harmony of families with autistic children? What outcomes are known to be associated with family harmony? and What about the models of family harmony? Method: This study uses Preferred Reporting Items for Systematic Reviews and Meta-Analysis for Protocols 2015 (PRISMA-P 2015) as a guideline. Search strategy in this study employs four electronic databases resources, namely Garuda Ristekdikti, ScienceDirect, ERIC, and psycINFO. Results: Among 15 articles which have been analyzed, there were 8 articles with quantitative study design and 1 article employing mixed-method study design. The rest of the six qualitative articles were correlation studies and four articles on family harmony models. Applications/Originality/Value: The conclusion from this research are the theories about family harmony (including determinants, influencing factors, aspects) are obtained through qualitative study with in depth interview and quantitative studyin families with different settings. Both studies, qualitative and quantitative study found yet about family harmony with autism children in Indonesia. Models of family harmony from qualitative study are obtained using in depth interview methods and from quantitative study are obtained by distributing questionaires to several families. These methods were approached by using the family role theory, interaction in family, and family relation theory. Research context and setting are dominant factors to determinate the models of family harmony.
Concussion in adolescence and the risk of multiple sclerosis: A retrospective cohort study
Authors: Povolo Christopher A , Reid Jennifer N , Shariff Salimah Z , Welk Blayne , Morrow Sarah A 
Published: February 2020

Background: Physical trauma, specifically concussions sustained during adolescence, has been hypothesized to be a risk factor for multiple sclerosis (MS). Objective: To examine the association between adolescent concussions and future MS diagnosis. Methods: This retrospective study using linked administrative databases from Ontario, Canada, identified 97,965 adolescents (age 11–18 years) who sustained ⩾1 concussion and presented to an emergency department between 1992 and 2011. Cases were matched 1:3 with individuals who had not sustained a concussion based on age, sex, address, and index date. The primary outcome was MS diagnosis, using a validated MS diagnosis definition: ⩾1 hospitalization or ⩾5 physician billings within 2 years. Results: A concussion during adolescence was associated with a significantly increased risk of MS (hazard ratio (HR) = 1.29, p = 0.03). Sex-specific analysis revealed that only males who sustained a concussion in adolescence had a raised risk of MS (HR = 1.41, p = 0.04). Conclusion: This study supports an association between concussions in adolescence and future MS diagnoses, highlighting the potentially serious long-term effects of concussions.
Cardiovascular outcomes with glucagon-like peptide-1 receptor agonists in patients with type 2 diabetes mellitus: A systematic review and meta-analysis
Authors: Pulipati Vishnu Priya , Ravi Venkatesh , Pulipati Priyanjali 
Published: February 2020

Background
Glucagon-like peptide-1 receptor agonists (GLP1RAs) are relatively newer anti-hyperglycemic agents, which have demonstrated cardiovascular benefits in patients with type 2 diabetes mellitus.

Design
We performed a meta-analysis of randomized controlled trials to evaluate the cardiovascular outcomes of GLP1RAs compared to placebo in type 2 diabetes mellitus patients. We performed an additional subgroup analysis to evaluate the role of GLP1RAs in patients with chronic kidney disease.

Methods
MEDLINE, Cochrane and ClinicalTrials.gov databases were searched from inception to 15 July 2019. The authors extracted relevant information from articles and independently assessed the study quality.

Results
Compared to placebo, GLP1RAs demonstrated a significant reduction in all-cause mortality (odds ratio (OR) 0.88, 95% confidence interval (CI) 0.82–0.95; P &lt; 0.001), cardiovascular mortality (OR 0.88, 95% CI 0.81–0.96; P = 0.004), primary composite endpoint (OR 0.86, 95% CI 0.80–0.91; P &lt; 0.001) and non-fatal stroke (OR 0.86, 95% 0.77–0.95; P = 0.004). There was no statistical difference in non-fatal myocardial infarction (OR 0.92, 95% CI 0.83–1.01; P = 0.09). In subgroup analyses of patients with estimated glomerular filtration rate less than 60 ml/min/1.73 m2 and less than 30 ml/min/1.73 m2, there was no significant difference in the primary composite endpoint.

Conclusions
GLP1RAs demonstrated a significant reduction in all-cause mortality, cardiovascular mortality, primary composite endpoint and non-fatal stroke in patients with type 2 diabetes mellitus. There was no significant difference in the primary composite endpoint in patients with type 2 diabetes mellitus and chronic kidney disease.
In‐Office Tympanostomy Tube Placement Using Iontophoresis and Automated Tube Delivery Systems
Authors: Yen David M. , Murray Michael T. , Puchalski Robert , Gould Andrew R. , Ansley John , Ow Randall A. , Moss Jonathan R. , England Laura J. , Syms Charles A. 
Published: February 2020

Objectives(1) To evaluate safety, tolerability, and technical success of lidocaine iontophoresis and a tympanostomy tube placement system for adults in an office setting and (2) to meet regulatory evidence requirements for new drugs and devices.Study DesignProspective, multicenter, single arm.SettingPatients were recruited in 8 community‐based practices in the United States between June and September 2017.Subjects and MethodsThis study evaluated tympanic membrane anesthesia and tube placement in 30 adults. Anesthesia was achieved via iontophoresis of a lidocaine/epinephrine solution. Tube placement was conducted using an integrated myringotomy and tube delivery system. Tolerability of tube placement was measured using a patient‐reported visual analog scale from 0 mm (no pain) to 100 mm (worst possible pain). Mean pain score was compared to a performance goal of 45 mm, where statistical superiority represents mild pain or less. Technical success and safety through 3 weeks postprocedure were evaluated.ResultsTwenty‐nine (29/30, 96.7%) patients had tube(s) successfully placed in all indicated ears. One patient demonstrated inadequate tympanic membrane anesthesia, and no tube placement was attempted. The mean (SD) pain score of 9.4 (15.7) mm was statistically superior to the performance goal. There were no serious adverse events. Seven nonserious events were related to device, procedure, or drug: inadequate anesthesia (1), vertigo (1), and dizziness (1) at the time of procedure and ear discomfort (1), tube occlusion (2), and medial tube migration (1) postprocedure.ConclusionLidocaine iontophoresis provides acceptable tympanic membrane anesthesia for safe, tolerable, and successful in‐office tube placement using an integrated myringotomy and tube delivery system.
Weighty choices: selecting optimal G-CSF doses for stem cell mobilization to optimize yield
Authors: Farhadfar Nosha , Hsu Jack W. , Logan Brent R. , Sees Jennifer A. , Chitphakdithai Pintip , Sugrue Michele W. , Abdel-Azim Hisham , Anderlini Paolo N. , Bredeson Christopher , Chhabra Saurabh , Diaz Miguel Angel , Ganguly Siddhartha , Hematti Peiman , Kamble Rammurti T. , Kasow Kimberly A. , Lazarus Hillard M. , Lynch Debra Kelly , Murthy Hemant S. , Olsson Richard F. , Papari Mona , Przepiorka Donna , Savani Bipin N. , Schears Raquel , Seo Sachiko , Solh Melhem M. , Spitzer Thomas , Yared Jean A. , Pulsipher Michael A. , Shah Nirali N. , Switzer Galen E. , Confer Dennis L. , Shaw Bronwen E. , Wingard John R. 
Published: February 2020

AbstractThere are limited data on the effect of donor body mass index (BMI) on peripheral blood stem cell (PBSC) mobilization response to granulocyte colony-stimulating factor (G-CSF), especially in unrelated donors. Obesity has been associated with persistent leukocytosis, elevated circulating progenitor cells, and enhanced stem cell mobilization. Therefore, we hypothesized that adequate collection of CD34+ cells may be achieved with lower doses (per kilogram of body weight) of G-CSF in donors with higher BMI compared with donors with lower BMI. Using the Center for International Blood and Marrow Transplant Research database, we evaluated the impact of donor BMI on G-CSF–mobilized PBSC yield in healthy unrelated donors. We examined 20 884 PBSC donations collected at National Marrow Donor Program centers between 2006 and 2016. We found significantly higher collection yields in obese and severely obese donors compared with normal and overweight donors. An increase in average daily G-CSF dose was associated with an increase in stem cell yield in donors with normal or overweight BMI. In contrast, an increase in average daily G-CSF dose beyond 780 μg per day in obese and 900 μg per day in severely obese donors did not increase cell yield. Pain and toxicities were assessed at baseline, during G-CSF administration, and postcollection. Obesity was associated with higher levels of self-reported donation-related pain and toxicities in the pericollection and early postdonation recovery periods. This study suggests a maximum effective G-CSF dose for PBSC mobilization in obese and severely obese donors, beyond which higher doses of G-CSF add no increased yield.
GNAS promotes inflammation-related hepatocellular carcinoma progression by promoting STAT3 activation
Authors: Ding Hongda , Zhang Xixia , Su Yang , Jia Changjun , Dai Chaoliu 
Published: February 2020

Abstract
Background
Hepatocellular carcinoma (HCC) is still the most common cause of cancer-related mortality worldwide and accumulating studies report that HCC is frequently linked to chronic inflammation. G-protein alpha-subunit (GNAS)-activating mutations have recently been reported to form a rare subgroup of inflammatory liver tumors. In this study, we investigated the roles of GNAS in inflammation-related HCC progression and its underlying mechanism.

Methods
Lipopolysaccharides (LPS) and diethylnitrosamine were employed to stimulate HCC cells to an induced inflammatory response. qRT-PCR, immunohistochemistry and immunoblotting were performed to detect the expression of GNAS in HCC tissues and cell lines. Expression levels of proinflammatory cytokines were detected by qRT-PCR and ELISA. N6-methyladenosine (m6A) methylation of GNAS mRNA was detected by RNA-binding protein immunoprecipitation (RIP). Transcription factors activation profiling plate array was performed to investigate the underlying mechanism in GNAS promoting interleukin-6 (IL-6) expression in HCC cells. HCC cell invasion was determined by transwell assay in vitro, and tumorigenesis was assessed with a subcutaneous xenograft mouse model of HCC.

Results
We found that LPS stimulation promotes GNAS expression in HCC cells through increasing m6A methylation of GNAS mRNA. The high expression level of GNAS promotes LPS-induced HCC cell growth and invasion by interacting with signal transducer and activator of transcription 3 (STAT3). Furthermore, GNAS knockdown inhibits LPS induced-IL-6 expression in HCC cells by suppressing STAT3 activation. Moreover, we found that GNAS promotes LPS-induced STAT3 activation in HCC cells through inhibiting long non-coding RNA TPTEP1 interacting with STAT3. In addition, GNAS expression promotes HCC development in mice and is related to poor survival.

Conclusions
Our findings for the first time indicate a tumor-promoting role of GNAS in inflammation-related HCC progression and provide a novel potential target for HCC therapy.
Prediction of new associations between ncRNAs and diseases exploiting multi-type hierarchical clustering
Authors: Barracchia Emanuele Pio , Pio Gianvito , D’Elia Domenica , Ceci Michelangelo 
Published: February 2020

Abstract
Background
The study of functional associations between ncRNAs and human diseases is a pivotal task of modern research to develop new and more effective therapeutic approaches. Nevertheless, it is not a trivial task since it involves entities of different types, such as microRNAs, lncRNAs or target genes whose expression also depends on endogenous or exogenous factors. Such a complexity can be faced by representing the involved biological entities and their relationships as a network and by exploiting network-based computational approaches able to identify new associations. However, existing methods are limited to homogeneous networks (i.e., consisting of only one type of objects and relationships) or can exploit only a small subset of the features of biological entities, such as the presence of a particular binding domain, enzymatic properties or their involvement in specific diseases.

Results
To overcome the limitations of existing approaches, we propose the system LP-HCLUS, which exploits a multi-type hierarchical clustering method to predict possibly unknown ncRNA-disease relationships. In particular, LP-HCLUS analyzes heterogeneous networks consisting of several types of objects and relationships, each possibly described by a set of features, and extracts multi-type clusters that are subsequently exploited to predict new ncRNA-disease associations. The extracted clusters are overlapping, hierarchically organized, involve entities of different types, and allow LP-HCLUS to catch multiple roles of ncRNAs in diseases at different levels of granularity. Our experimental evaluation, performed on heterogeneous attributed networks consisting of microRNAs, lncRNAs, diseases, genes and their known relationships, shows that LP-HCLUS is able to obtain better results with respect to existing approaches. The biological relevance of the obtained results was evaluated according to both quantitative (i.e., TPR@k, Areas Under the TPR@k, ROC and Precision-Recall curves) and qualitative (i.e., according to the consultation of the existing literature) criteria.

Conclusions
The obtained results prove the utility of LP-HCLUS to conduct robust predictive studies on the biological role of ncRNAs in human diseases. The produced predictions can therefore be reliably considered as new, previously unknown, relationships among ncRNAs and diseases.
Combining feature selection and shape analysis uncovers precise rules for miRNA regulation in Huntington’s disease mice
Authors: Mégret Lucile , Nair Satish Sasidharan , Dancourt Julia , Aaronson Jeff , Rosinski Jim , Neri Christian 
Published: February 2020

Abstract
Background
MicroRNA (miRNA) regulation is associated with several diseases, including neurodegenerative diseases. Several approaches can be used for modeling miRNA regulation. However, their precision may be limited for analyzing multidimensional data. Here, we addressed this question by integrating shape analysis and feature selection into miRAMINT, a methodology that we used for analyzing multidimensional RNA-seq and proteomic data from a knock-in mouse model (Hdh mice) of Huntington’s disease (HD), a disease caused by CAG repeat expansion in huntingtin (htt). This dataset covers 6 CAG repeat alleles and 3 age points in the striatum and cortex of Hdh mice.

Results
Remarkably, compared to previous analyzes of this multidimensional dataset, the miRAMINT approach retained only 31 explanatory striatal miRNA-mRNA pairs that are precisely associated with the shape of CAG repeat dependence over time, among which 5 pairs with a strong change of target expression levels. Several of these pairs were previously associated with neuronal homeostasis or HD pathogenesis, or both. Such miRNA-mRNA pairs were not detected in cortex.

Conclusions
These data suggest that miRNA regulation has a limited global role in HD while providing accurately-selected miRNA-target pairs to study how the brain may compute molecular responses to HD over time. These data also provide a methodological framework for researchers to explore how shape analysis can enhance multidimensional data analytics in biology and disease.
Comparative analysis of basic helix–loop–helix gene family among Brassica oleracea, Brassica rapa, and Brassica napus
Authors: Miao Liming , Gao Yingying , Zhao Kun , Kong Lijun , Yu Shubo , Li Rongrong , Liu Kaiwen , Yu Xiaolin 
Published: February 2020

AbstractBackgroundThe basic helix–loop–helix (bHLH) is the second largest gene family in the plant, some members play important roles in pistil development and response to drought, waterlogging, cold stress and salt stress. The bHLH gene family has been identified in many species, except forBrassica oleraceaandB. napusthus far.This study aims to identify the bHLH family members inB. oleracea,B. rapaandB. napus, and elucidate the expression, duplication, phylogeny and evolution characters of them.ResultA total of 268 bHLH genes inB. oleracea, 440 genes inB. napus, and 251 genes inB. rapa, including 21 new bHLH members, have been identified.Subsequently, the analyses of the phylogenetic trees, conserved motifs and gene structures showed that the members in the same subfamily were highly conserved. MostKa/Ksvalues of homologous gene were < 1, which indicated that these genes suffered from strong purifying selection for retention. The retention rates ofBrabHLHandBolbHLHgenes were 51.6 and 55.1%, respectively. The comparative expression patterns betweenB. rapaandB. napusshowed that they had similar expression patterns in the root and contrasting patterns in the stems, leaves, and reproductive tissues. In addition, there were 41 and 30 differential expression bHLH genes under the treatments of ABA and JA, respectively, and the number of down regulation genes was significantly more than up regulation genes.ConclusionIn the present study, we identified and performed the comparative genomics analysis of bHLH gene family amongB. oleracea,B. rapaandB. napus, and also investigated their diversity. The expression patterns betweenB. rapaandB. napusshows that they have the similar expression pattern in the root and opposite patterns in the stems, leaves, and reproduction tissues. Further analysis demonstrated that some bHLH gene members may play crucial roles under the abiotic and biotic stress conditions. This is the first to report on the bHLH gene family analysis inB. oleraceaandB. napus,which can offer useful information on the functional analysis of the bHLH gene in plants.