bluuebunny/crossref_metadata_2025_split huggingface dataset:
A chemiluminescent protease probe for rapid, sensitive, and inexpensive detection of liveMycobacterium tuberculosis
Authors: Babin Brett M. , Fernandez-Cuervo Gabriela , Sheng Jessica , Green Ori , Ordonez Alvaro A. , Turner Mitchell L. , Keller Laura J. , Jain Sanjay K. , Shabat Doron , Bogyo Matthew 
Published: September 2020

AbstractTuberculosis (TB) is a top-ten cause of death worldwide. Successful treatment is often limited by insufficient diagnostic capabilities, especially at the point of care in low-resource settings. The ideal diagnostic must be fast, cheap, and require minimal clinical resources while providing high sensitivity, selectivity, and the ability to differentiate live from dead bacteria. We describe here the development of a Fast, Luminescent, and Affordable Sensor of Hip1 (FLASH) for the diagnosis and monitoring of drug sensitivity ofMycobacterium tuberculosis(Mtb). FLASH is a selective chemiluminescent substrate for theMtbprotease Hip1 that when processed, produces visible light that can be measured with a high signal to noise ratio using inexpensive sensors. FLASH is sensitive to fmol of recombinant Hip1 enzymein vitroand can detect as few as thousands ofMtbcells in culture or in human sputum samples within minutes. The probe is highly selective forMtbcompared to other non-tuberculous mycobacteria and can distinguish live from dead cells. Importantly, FLASH can be used to measure antibiotic killing ofMtbin culture with greatly accelerated timelines compared to traditional protocols. Overall, FLASH has the potential to enhance both TB diagnostics and drug resistance monitoring in resource-limited settings.One Sentence SummaryA luminescent probe enables sensitive detection ofMycobacterium tuberculosisfor diagnostics, treatment monitoring, and drug susceptibility testing.
Functional Brain Networks and Alcohol Consumption: From the Naïve State to Chronic Heavy Drinking
Authors: Rowland Jared A. , Stapleton-Kotloski Jennifer R. , Alberto Greg E. , Davenport April T. , Epperly Phillip M. , Godwin Dwayne W. , Daunais James B. 
Published: September 2020

AbstractA fundamental question for alcohol use disorder is how naïve brain networks are reorganized in response to the consumption of alcohol. The current study aimed to determine the progression of alcohol’s effect on functional brain networks during the transition from naïve, to early, to chronic consumption. Resting-state brain networks of six female monkeys were acquired using magnetoencephalography prior to alcohol exposure, after early exposure, and after free-access to alcohol using a well-established model of chronic heavy alcohol use. Functional brain network metrics were derived at each time point. Assortativity, average connection frequency, and number of gamma connections changed significantly over time. All metrics remained relatively stable from naïve to early drinking, and displayed significant changes following increased quantity of alcohol consumption. The assortativity coefficient was significantly less negative (p=.043), connection frequency increased (p=.03), and gamma connections increased (p=.034). Further, brain regions considered hubs (p=.037) and members of the Rich Club (p=.012) became less common across animals following the introduction of alcohol. The minimum degree of the Rich Club prior to alcohol exposure was significantly predictive of future free-access drinking (r=-.88,p<.001). Results suggest naïve brain network characteristics may be used to predict future alcohol consumption, and that alcohol consumption alters the topology of functional brain networks, shifting hubs and Rich Club membership away from previous regions in a non-systematic manner. Further work to refine these relationships may lead to the identification of a high-risk AUD phenotype.
Dynamic gene expression and growth underlie cell-to-cell heterogeneity inEscherichia colistress response
Authors: Sampaio Nadia M. V. , Blassick Caroline M. , Lugagne Jean-Baptiste , Dunlop Mary J. 
Published: September 2020

AbstractCell-to-cell heterogeneity in gene expression and growth can have critical functional consequences, such as determining whether individual bacteria survive or die following stress. Although phenotypic variability is well documented, the dynamics that underlie it are often unknown. This information is important because dramatically different outcomes can arise from gradual versus rapid changes in expression and growth. Using single-cell time-lapse microscopy, we measured the temporal expression of a suite of stress response reporters inEscherichia coli, while simultaneously monitoring growth rate. In conditions without stress, we found several examples of pulsatile expression. Single-cell growth rates were often anti-correlated with gene expression, with changes in growth preceding changes in expression. These expression and growth dynamics have functional consequences, which we demonstrate by measuring survival after challenging cells with the antibiotic ciprofloxacin. Our results suggest that fluctuations in gene expression and growth dynamics in stress response networks can have direct consequences for survival.
Cell-extrinsic effects in T cell acute lymphoblastic pre-leukemia stem cells mediated by EphA3
Authors: Zamora Adriana C. Pliego , Ranasinghe Hansini , Lisle Jessica E. , Huang Stephen , Wadlow Racheal , Scott Andrew M. , Boyd Andrew W. , Slape Christopher I. 
Published: September 2020

AbstractOur recent study of a novel model of T-ALL pre-leukemic stem cells, the NUP98-HOXD13 (NHD13) mouse, showed that the abnormal self-renewal of these stem cells was dependent on Lyl1 yet, when Lyl1 was deleted, the T-ALL still developed. In the present study, we observe that the thymocytes in these mice also overexpress EphA3, and we characterise the thymocytes in NHD13-EphA3−/−mice. NHD13-EphA3−/−thymocytes retain their abnormal self-renewal activity demonstrated by their capacity to engraft following primary and secondary transplants. Strikingly, NHD13-EphA3−/−thymocytes fail to engraft upon the third serial transplant, whereas the NHD13 thymocytes engraft indefinitely. Seeking to explain this, we find that NHD13 DN2 thymocytes are capable of halting the normal differentiation process of incoming WT progenitor cells, and remarkably, this capacity is severely impaired in the absence of EphA3. Therefore EphA3 is not critical for engraftment, but is essential for enabling the halt in differentiation of neighbouring WT cells, which in turn allows the incumbent progenitors to remain longer in the thymus due to an absence of normal cell competition, a property that in itself has been demonstrated to be oncogenic. We suggest that pre-leukemic self-renewal in this model is a complex interplay of cell intrinsic and extrinsic factors, and that multiple redundant pathways to leukemogenesis are active in this model.
Phosphorylation of luminal region of the SUN-domain protein Mps3 promotes nuclear envelope localization during meiosis
Authors: Rao H. B. D. Prasada , Sato Takeshi , Challa Kiran , Fujita Yurika , Shinohara Miki , Shinohara Akira 
Published: September 2020

AbstractDuring meiosis, protein ensembles in the nuclear envelope (NE) containing SUN- and KASH-domain proteins, called linker nucleocytoskeleton and cytoskeleton (LINC) complex, promote the chromosome motion. Yeast SUN-domain protein, Mps3, forms multiple meiosis-specific ensembles on NE, which show dynamic localisation for chromosome motion; however, the mechanism by which these Mps3 ensembles are formed during meiosis remains largely unknown. Here, we showed that the cyclin-dependent protein kinase (CDK) and Dbf4-dependent Cdc7 protein kinase (DDK) regulate meiosis-specific dynamics of Mps3 on NE, particularly by mediating the resolution of Mps3 clusters and telomere clustering. We also found that the luminal region of Mps3 juxtaposed to the inner nuclear membrane is required for meiosis-specific localisation of Mps3 on NE. Negative charges introduced by meiosis-specific phosphorylation in the luminal region of Mps3 alter its interaction with negatively charged lipids by electric repulsion in reconstituted liposomes. Phospho-mimetic substitution in the luminal region suppresses the localisation of Mps3 via the inactivation of CDK or DDK. Our study revealed multi-layered phosphorylation-dependent regulation of the localisation of Mps3 on NE for meiotic chromosome motion and NE remodelling.
The coiled-coil domain of glycosomal membrane-associatedLeishmania donovaniPEX14: cloning, overexpression, purification and preliminary crystallographic analysis
Authors: Shakya Anil Kumar , Pratap J. Venkatesh 
Published: September 2020

The glycosomal membrane-associatedLeishmania donovaniprotein PEX14, which plays a crucial role in protein import from the cytosol to the glycosomal matrix, consists of three domains: an N-terminal domain where the signalling molecule binds, a transmembrane domain and an 84-residue coiled-coil domain (CC) that is responsible for oligomerization. CCs are versatile domains that participate in a variety of functions including supramolecular assembly, cellular signalling and transport. Recombinant PEX14 CC was cloned, overexpressed, affinity-purified with in-column thrombin cleavage and further purified by size-exclusion chromatography. Crystals that diffracted to 1.98 Å resolution were obtained from a condition consisting of 1.4 Msodium citrate tribasic dihydrate, 0.1 MHEPES buffer pH 7.5. The crystals belonged to the monoclinic space groupC2, with unit-cell parametersa = 143.98,b= 32.62,c= 95.62 Å, β = 94.68°. Structure determination and characterization are in progress.
Converging and diverging business model innovation in regional intersectoral cooperation–exploring wine industry 4.0
Authors: Dressler Marc , Paunovic Ivan 
Published: September 2020

PurposeIndustry 4.0, the integrated digital process world in production, is an under researched area. Especially for the wine industry, 4.0 theoretical base up to date is weak, impeding further digital transformation of the wine industry. Some basic-level digital capabilities exist in most of the researched business models, which is why further digital transformation requires strategic redefining of business models in order to further transform the whole industry.Design/methodology/approachA qualitative research approach based on the concept of business model innovation (BMI) has been deployed in order to identify business models and consensus building between industry´s stakeholders. Semistructured interviews delivered primary data.FindingsThe diversity of existing business models in the wine industry as well regarding BMI is confirmed, where each one occupies a specific place in the innovation ecosystem for Industry 4.0.Research limitations/implicationsThis qualitative research is exploratory and directed toward theory building. The findings confirm the importance of environmental scanning and market sensing for convergent technologies as well as the need to develop core value proposition.Practical implicationsThis study has important implications for companies and their capabilities inside wine industry 4.0, while its main practical value being a systematical organization of weak signals to support small entrepreneurs’ transition to digitalization and Industry 4.0Originality/valueThe paper nourishes the theory on innovation trajectories for Industry 4.0 by including previously unidentified BMIs and a convergence–divergence continuum on the example of wine Industry 4.0.
A Study on the Efficiency in Korea Foreign Exchange Market
Authors: Kang Seok Kyu 
Published: September 2020

This study is to examine the three theme of the eπiciency of Korea foreign exchange market including the unbiasedness testing, the relative efficiency estimates, and the information spillover efficiency. Data using the analysis 81’e won-dollar spot and futures in domestic and won-dollar forward in offshore. i.e.. New York and Singapore NDF (non-delivery forward).
The empirical results are summarized as follows: First. the efficient market or unbiasedness expectations hypothesis is not rejected in the won-dollar currency futures market apart from offshore New York and Singapore NDF markets. This indicates that the won-dollar futures price is likely to be an accurate indicator of future won-dollar spot prices without the trader having to pay a risk premium for the privilege of trading the contract. Second. the findings suggest the domestic won-dollar futures market is 13.58% efficient. the Singapore offshore won-dollar NDF market is 11.38% efficient. and the New York offshore won-dollar NDF market is 2.68% efficient. This indicates that the domestic won-dollar futures market is more efficient than the offshore won-dollar NDF market. It is therefore possible to conclude that the domestic currency futures price is a relatively successful predictor of the future spot price. Third. the findings suggest the information spillover exists between domestic won-dollar spot/futures market and offshore won-dollar New York NDF market in both direction. This indicates that the two markets are efficiently linked.
Futures and Option Prices After the Stock Market Close: Evidence from the Korean Markets
Authors: Lee Jae Ha , Kwon Sang Soo 
Published: September 2020

In the KOSPI2oo futures and option markets. additional fifteen minutes (15 : 00∼15 개5) after the underlying stock market close are given tor the adjustments of the futures and option positions. During the first five minutes. 15: 00∼15 : 05. a continuous auction trading is made. while the trading at a single clearing price is made for the remaining ten minutes. 15: 05∼15: 15.
Previous studies focused on the synchronous trading in terms of transaction time in the analysis of the lead-lag relationship. truncating the futures and option data during 15 : 00∼15 : 15. In this article. we explore how the KOSPI2oo futures and option returns for the extra fifteen minutes impact the next day's KOSPI200 cash returns, We also examine the lead-lag relationship during the reggular trading hours (9 : 00∼15 : 00) and the impact of the cash returns during 14 : 20∼15 : 00 on futures and option returns during 15 : 00∼15: 15. Our main findings are summarized as follows.
First. the KOSPI200 futures and option returns during 15 : 00∼15 : 15 lead the close-to-open KOSPI200 cash return, even though the trading volume and return volatility during 15: 00∼15: 15 are lower relative to the regular stock market session (9 : 00∼15: 00). The impact of the futures and option returns on the cash return lasts hlK) minutes and one minute‘ repectively. after the next day open. Second. the option return during the continuous auction trading session (15 : 00∼ 15 : 05) leads the close-to-open cash return. while the futures return of trading at a single clearing price during 15 : 05∼15 : 10 impacts the close-to-open cash return. Third, we found that the lead-lag relationships among the KOSPI200 futures, option, and cash returns are not constant during the reg비ar stock market session‘ In partieular. the impact of the KOSPI200 cash ret un during 14 : 40∼15 : 00 on the futures and option retuns for the 15 : 00∼15: 15 Interval is much stronger. compared with other time zones.
Finally. the KOSPI200 cash return during the last ten minutes of trading at a Single clearing price (14 : 50∼15 : 00). significantly impacts the option return during 15: 00∼15: 05. while there is no impact on the futures return (15 : 00∼15: 15).
Effects of Introducing Equity-Linked Warrants on Stock Market Behavior
Authors: Ryu Doojin 
Published: September 2020

This paper investigates the effects of introducing equity-linked warrants (ELWs) on the stock price, trading volume, volatility, and systematic risk (beta) by using the event study methodology. The study defines the event date as the announcement date as well as the listing date. In addition, whereas previous research has investigated only call ELWs, this study analyzes the effects of introducing both call and put ELWs.
The results provide no evidence of hedging effects of issuers before the announcement dates and information effects after the announcement dates. In addition, we can't find any significant changes of variables associated with the market completeness hypothesis near the listing dates.
However, the trading volume of the stock tends to increase in the days immediately following the listing of call ELWs, which may be due to the “informed trading effect”. The empirical results also provide support for the “diminishing short-sales restrictions” hypothesis related to the listing of put ELWs, which implies that short-sale restrictions can be reduced because put ELWs can provide investors with short positions in the underlying stock.
Effect of Prepayment and Call Limit on the Duration of CMO (Multi-tranche MBS): Full vs. Partial Pass-Through Structure
Authors: Park Yun Woo , Bang Doo Won 
Published: September 2020

Residential mortgage loans as well as the MBS (mortgage-backed security), which securitizes these loans, are exposed to prepayment risk. We examine the effect of prepayment process on the duration of the CMO (multi-tranche MBS). In particular, we examine the effect of partial pass-through where there is a call limit expressed as a percentage of initial tranche balance. Due to the absence of empirical research on the CMO duration, neither the actual CMO duration nor the determinants of the CMO duration have been reported. Our study reports the actual CMO duration and the determinants of the CMO duration. By showing that the CMO duration is much shorter than the nominal time-to-maturity we point to the need to search for longer duration MBS structures. We find that in both the deterministic and stochastic interest rate environments duration is reduced as prepayment speed rises and duration rises as call limit decreases.
We make contribution to the literature by shedding light on the effect of prepayment and call limit on the duration of multi-tranche MBS. In particular, this research characterizes the impact of the partial pass-through structuring approach on the CMO duration as well as CMO pricing. Finally, it assists CMO investors in better assessing and managing reinvestment risks of pass-through products.
Evaluation of multi-level intervention strategies for a psychologically healthy construction workplace in Nigeria
Authors: Nwaogu Janet Mayowa , Chan Albert P.C. 
Published: September 2020

PurposeThe need to improve the mental health of construction personnel has increased owing to high rates of mental health problems. Hence, a proper evaluation of a mix of implementable intervention strategies in the workplace will assist in achieving good mental health. Although there are recommendations in occupational health literature on strategies that can be adopted, it is unknown how they fit appropriately into the construction industry. Therefore, the purpose of this study is to identify the mix of strategies for the construction industry and their criticality.Design/methodology/approachDrawing from the context of developing countries with Nigeria as a case study, data was collected using the quantitative technique. A questionnaire survey consisting of 31 intervention strategies was administered to a purposive sample of 45 experts in the Nigerian construction industry. The data collected was analyzed using mean score analysis and fuzzy synthetic evaluation.FindingsThe study revealed that strategies focused on boosting employee morale and engagement and interpersonal relationship offer higher chances of improving mental health among construction personnel. The study showed that implementing job crafting and sculpting may benefit the industry. The analysis showed that the overall criticality of the intervention strategies to the Nigerian construction workplace is high, suggesting that if implemented, the mental health of construction personnel can be improved.Originality/valueThe study provides an initial understanding of the most critical multi-level intervention strategies to enhance good mental health among construction personnel in Nigeria and the global construction industry. These findings serve as a guide to policymakers and advocate the implementation of strategies to adopt for a psychologically healthy construction workplace in developing countries.
Changing the quality mindset at Ashok Leyland (Parts A and B)
Authors: M.V. Anuradha , C.R. Rajan , Ganduri Uma Rao 
Published: September 2020

Theoretical basis
Change in culture brought about by effective leadership is at the core of this case. Therefore, two broad topics can be discussed using this case: organizational culture change and Change Leadership OR Role of leaders in organzational change.

Research methodology
The case was prepared using primary data collected through a series of interviews conducted with participants of the change process. The participants included R. Sivanesan, Senior Vice President (Quality, Sourcing and Supply Chain) of Ashok Leyland, many members of the quality team, production department, HR executives and members of the marketing team. Secondary data in the form of an interview of Mr Vinod Dasari published in a popular magazine Autocar Professionals and organizational documents/presentations used during the change process were also used to build the case.

Case overview/synopsis
In 2011, when Vinod Dasari took over as the Managing Director and CEO of Ashok Leyland (AL), he hired R. Sivanesan. The quality standards of the vehicles produced in the AL plants in 2011 was far from satisfactory. He decided to change this. Part A of the case discusses the challenges faced by Sivanesan and Vinod Dasari in bringing about a change in the quality management practices at AL. Part B discusses the steps they actually took and the change that resulted from it.

Learning objectives
At the end of the case discussion, the participants will be able to develop an understanding of the various aspects of organizational culture and how it manifests itself; become aware of the underlying causes of resistance to change; critically evaluate and apply various theories of change management; create an action plan for changing the culture of any organization; and appreciate the role of leaders as change agents.

Complexity academic level
The central theme in this case is managing culture change within organizations through effective leadership. Instructors teaching courses in organizational theory, organization structure/culture and leadership will find this case relevant. It is primarily intended for use in MBA and Executive Education programs in Management.
The effect of residents’ perception of hotel social responsibility on overall attitude toward tourism
Authors: Boğan Erhan , Dedeoğlu Bekir Bora , Balikçioğlu Dedeoğlu Sevgi 
Published: September 2020

Purpose
This paper aims to investigate the moderating effects of residents’ demographic attributes on the relationship between perceptions of hotels’ social responsibility (HSR) practices and overall attitude toward tourism.

Design/methodology/approach
Perceptions of HSR were examined via social, economic and environmental dimensions. The research model was investigated with structural equation modeling and multigroup analyses.

Findings
The findings show that residents’ perceptions of HSR had a positive impact on their attitude toward tourism and that their demographic attributes had a moderating effect on this relationship.

Originality/value
Although there have been many papers on stakeholders’ returns to corporate social responsibility practices implemented by hotels, residents’ returns to these practices are insufficiently investigated. Moreover, to the best of the authors’ knowledge, there is no research on the moderating effect of residents’ demographic attributes on the relationship between perceived social responsibility and overall attitude toward tourism.