bluuebunny/crossref_metadata_2025_split huggingface dataset:
The life cycle of cyclotides: biosynthesis and turnover in plant cells
Authors: Slazak Blazej , Haugmo Tobias , Badyra Bogna , Göransson Ulf 
Published: July 2020

Abstract
Key message
Turnover rates have implications for understanding cyclotide biology and improving plant cell culture-based production systems.

Abstract
Cyclotides are a family of polypeptides recognized for a broad spectrum of bioactivities. The cyclic, cystine knot structural motif imparts these peptides with resistance to temperature, chemicals and proteolysis. Cyclotides are found widely distributed across the Violaceae and in five other plant families, where their presumed biological role is host defense. Violets produce mixtures of different cyclotides that vary depending on the organ, tissue or influence of environmental factors. In the present study, we investigated the biosynthesis and turnover of cyclotides in plant cells. Viola uliginosa suspension cultures were grown in media where all nitrogen containing salts were replaced with their 15N counterparts. This approach combined with LC–MS analysis allowed to separately observe the production of 15N-labelled peptides and decomposition of 14N cyclotides present in the cells when switching the media. Additionally, we investigated changes in cyclotide content in V. odorata germinating seeds. In the suspension cultures, the degradation rates varied for individual cyclotides and the highest was noted for cyO13. Rapid increase in production of 15N peptides was observed until day 19 and subsequently, a plateau of production, indicating an equilibrium between biosynthesis and turnover. The developing seedling appeared to consume cyclotides present in the seed endosperm. We show that degradation processes shape the cyclotide pattern present in different tissues and environments. The results indicate that individual cyclotides play different roles—some in defense and others as storage proteins. The turnover of cyclotides should be accounted to improve cell culture production systems.
Current and future potential of solar and wind energy over Africa using the RegCM4 CORDEX-CORE ensemble
Authors: Sawadogo Windmanagda , Reboita Michelle Simões , Faye Aissatou , da Rocha Rosmeri Porfírio , Odoulami Romaric C. , Olusegun Christiana F. , Adeniyi Mojisola Oluwayemisi , Abiodun Babatunde J. , Sylla Mouhamadou Bamba , Diallo Ismaila , Coppola Erika , Giorgi Filippo 
Published: July 2020

AbstractRenewable energy is key for the development of African countries, and knowing the best location for the implementation of solar and wind energy projects is important within this context. The purpose of this study is to assess the impact of climate change on solar and wind energy potential over Africa under low end (RCP2.6) and high end (RCP8.5) emission scenarios using a set of new high resolution (25 km) simulations with the Regional Climate Model version 4 (RegCM4) produced as part of the CORDEX-CORE initiative. The projections focus on two periods: (i) the near future (2021–2040) and ii) the mid-century future (2041–2060). The performance of the RegCM4 ensemble mean (Rmean) in simulating relevant present climate variables (1995–2014) is first evaluated with respect to the ERA5 reanalysis and satellite-based data. The Rmean reproduces reasonably well the observed spatial patterns of solar irradiance, air temperature, total cloud cover, wind speed at 100 m above the ground level, photovoltaic power potential (PVP), concentrated solar power output (CSPOUT) and wind power density (WPD) over Africa, though some biases are still evident, especially for cloud-related variables. For the future climate, the sign of the changes is consistent in both scenarios but with more intense magnitude in the middle of the century RCP8.5 scenario. Considering the energy variables, the Rmean projects a general decrease in PVP, which is more pronounced in the mid-century future and under RCP8.5 (up to 2%). Similarly, a general increase in CSPOUT (up to 2%) is projected over the continent under both the RCP2.6 and RCP8.5 scenarios. The projection in WPD shows a similar change (predominant increase) in the near and mid-century future slices under both RCPs with a maximum increase of 20%. The present study suggests that the RCP2.6 emission scenario, in general, favours the implementation of renewable energy in Africa compared to the RCP8.5.
Changes in functional connectivity after theta-burst transcranial magnetic stimulation for post-traumatic stress disorder: a machine-learning study
Authors: Zandvakili Amin , Swearingen Hannah R. , Philip Noah S. 
Published: July 2020

AbstractIntermittent theta burst stimulation (iTBS) is a novel treatment approach for post-traumatic stress disorder (PTSD), and recent neuroimaging work indicates that functional connectivity profiles may be able to identify those most likely to respond. However, prior work has relied on functional magnetic resonance imaging, which is expensive and difficult to scale. Alternatively, electroencephalography (EEG) represents a different approach that may be easier to implement in clinical practice. To this end, we acquired an 8-channel resting-state EEG signal on participants before (n = 47) and after (n = 43) randomized controlled trial of iTBS for PTSD (ten sessions, delivered at 80% of motor threshold, 1,800 pulses, to the right dorsolateral prefrontal cortex). We used a cross-validated support vector machine (SVM) to track changes in EEG functional connectivity after verum iTBS stimulation. We found that an SVM classifier was able to successfully separate patients who received active treatment vs. sham treatment, with statistically significant findings in the Delta band (1–4 Hz, p = 0.002). Using Delta coherence, the classifier was 75.0% accurate in detecting sham vs. active iTBS, and observed changes represented an increase in functional connectivity between midline central/occipital and a decrease between frontal and central regions. The primary limitations of this work are the sparse electrode system and a modest sample size. Our findings raise the possibility that EEG and machine learning may be combined to provide a window into mechanisms of action of TMS, with the potential that these approaches can inform the development of individualized treatment methods.
Oxidative stress biomarkers in Fabry disease: is there a room for them?
Authors: Simoncini C. , Torri S. , Montano V. , Chico L. , Gruosso F. , Tuttolomondo A. , Pinto A. , Simonetta I. , Cianci V. , Salviati A. , Vicenzi V. , Marchi G. , Girelli D. , Concolino D. , Sestito S. , Zedde M. , Siciliano G. , Mancuso Michelangelo 
Published: July 2020

AbstractBackgroundFabry disease (FD) is an X-linked lysosomal storage disorder, caused by deficient activity of the alpha-galactosidase A enzyme leading to progressive and multisystemic accumulation of globotriaosylceramide. Recent data point toward oxidative stress signalling which could play an important role in both pathophysiology and disease progression.MethodsWe have examined oxidative stress biomarkers [Advanced Oxidation Protein Products (AOPP), Ferric Reducing Antioxidant Power (FRAP), thiolic groups] in blood samples from 60 patients and 77 healthy controls.ResultsAOPP levels were higher in patients than in controls (p < 0.00001) and patients presented decreased levels of antioxidant defences (FRAP and thiols) with respect to controls (p < 0.00001). In a small group of eight treatment-naïve subjects with FD-related mutations, we found altered levels of oxidative stress parameters and incipient signs of organ damage despite normal lyso-Gb3 levels.ConclusionsOxidative stress occurs in FD in both treated and naïve patients, highlighting the need of further research in oxidative stress-targeted therapies. Furthermore, we found that oxidative stress biomarkers may represent early markers of disease in treatment-naïve patients with a potential role in helping interpretation of FD-related mutations and time to treatment decision.
Computerized clinical decision system and mobile application with expert support to optimize management of vertigo in primary care: study protocol for a pragmatic cluster-randomized controlled trial
Authors: Filippopulos Filipp M. , Huppert Doreen , Brandt Thomas , Hermann Margit , Franz Mareike , Fleischer Steffen , Grill Eva 
Published: July 2020

AbstractVertigo and dizziness are amongst the most common symptoms in medicine and often have a major impact on activities of daily life. Although many causes of vertigo and dizziness can easily be recognized, patients often receive inappropriate and ineffective treatment. The reasons for this are various. Because vertigo/dizziness is an interdisciplinary symptom and there is a lack of standardised diagnostic tools, it is easy to lose the overview of the possible differential diagnoses. There is evidence though, that the management of patients with vertigo/dizziness can be optimized using standardized care pathways with digital support. The present study (within the framework of “PoiSe—prevention, online feedback, and interdisciplinary therapy of acute vestibular syndromes by e-health”) aims to evaluate the implementation of a program with several interlocking components. The three main components are a computerized clinical decision system, a mobile application, a counselling and interdisciplinary educational program developed by the German Center for Vertigo and Balance Disorders (DSGZ). The study is a cluster-randomized controlled trial with a parallel-group design, as well as a detailed process evaluation. Clusters comprise of primary care physician practices in Bavaria, Germany. In the scope of the study the effectiveness, acceptability and efficiency of the intervention will be evaluated. It is anticipated that the intervention will improve the quality and efficiency of the management of dizzy patients. A higher diagnostic accuracy, optimized treatment, and disease progression monitoring is expected to improve patient-relevant outcomes and reduce health-care costs.
Augmentation of soft tissue volume at pontic sites: a comparison between a cross-linked and a non-cross-linked collagen matrix
Authors: Naenni Nadja , Walter Prisca , Hämmerle Christoph H. F. , Jung Ronald E. , Thoma Daniel S. 
Published: July 2020

Abstract
Aim
To assess histopathological and histomorphometric outcomes of soft tissue volume augmentation procedures at pontic sites using a volume-stable cross-linked collagen matrix (VCMX) and a non-cross-linked collagen matrix (XCM).

Materials and methods
In twelve adult beagle dogs, the mandibular premolars and first molar were hemisected and the mesial root extracted. Soft tissue augmentation was randomly performed using VCMX, XCM, or a sham-operated control. Sacrifice was performed after 4, 8, and 26 weeks. Non-decalcified sections were analyzed for histopathologic and histomorphometric measurements at four different levels below the crest (1.5, 2.5, 3.5, and 5.5 mm).

Results
Group VCMX presented a greater overall amount of soft tissue at all healing time points, more pronounced fibroblast ingrowth, vascularization, and a substantial new collagen deposition. Over time, group XCM demonstrated faster signs of degradation compared with group VCMX. Four weeks after augmentation, group VCMX yielded a higher mean ridge width compared with groups XCM (2.22 mm VCMX, 0.89 mm XCM (at 2.5 mm); 2.05 mm VCMX, 0.80 mm XCM (at 3.5 mm) p < 0.05) and sham (0.59 mm sham (at 1.5 mm); 0.48 mm (at 2.5 mm); 0.44 mm (at 3.5 mm) p < 0.05). After healing periods of 8 and 26 weeks, measurements in group VCMX remained significantly higher compared with group sham both at 8 weeks (levels of 1.5 mm, 2.5 mm and 5.5 mm) and at 26 weeks (levels of 1.5 mm, 3.5 mm and 5.5 mm) (p < 0.05).

Conclusion
The use of a cross-linked collagen matrix resulted in a greater and more stable ridge width over time compared with control groups.

Clinical relevance
Soft tissue volume augmentation at pontic sites is more effective when using a cross-linked compared with a non-cross-linked collagen matrix.
Inverse optimization approach to the identification of electricity consumer models
Authors: Kovács András 
Published: July 2020

AbstractStackelberg game models for demand response management in smart electricity grids have been studied extensively in the scientific literature. Still, a barrier to their practical applicability is the assumption that the retailer (leader in the game) has perfect knowledge about the consumers’ (followers’) decision model. This paper investigates the possibilities of reconstructing the consumers’ decision model from historic tariff and consumption data. For this purpose, it introduces an inverse optimization approach to eliciting the parameters of electricity consumer models formulated as linear programs from the historic samples. The inverse problem is first transformed into a quadratically constrained quadratic program, and then solved using successive linear programming techniques. The approach is demonstrated on a common consumer model with multiple types of deferrable loads behind a single smart meter. Experimental results are presented, and directions for future research are proposed.
Choosing a Smuggler: Decision-making Amongst Migrants Smuggled to Europe
Authors: Campana Paolo , Gelsthorpe Loraine 
Published: July 2020

AbstractThis paper explores how transactions between smugglers and migrants come about in the context of irregular migration. We first offer some theoretical reflections on the challenges that such a context poses to both parties—smugglers and migrants—and point to three main conditions under which smuggling transactions take place: illegality, asymmetrical information and low trust. Next, we explore the strategies that migrants and smugglers alike may employ to overcome these challenges. We focus on three broad sets of strategies related to information-gathering, to information-checking (reputation and the role of physical and virtual communities) and to developing substitutes for trust (guarantees, escrow services and hostage-taking strategiesà laThomas Schelling). To illustrate our reasoning, we draw on examples from published works as well as from a novel set of 43 qualitative interviews with migrants recently smuggled to Europe. Such interviews were carried out in a destination country (England) and in a transit country (Greece). Evidence from this work supports previous calls to move away from a simplistic “predator-victim” discourse.
The Coxeter relations and KP map for non-commuting symbols
Authors: Doliwa Adam , Noumi Masatoshi 
Published: July 2020

AbstractWe give an action of the symmetric group on non-commuting indeterminates in terms of series in the corresponding Mal’cev–Newmann division ring. The action is constructed from the non-Abelian Hirota–Miwa (discrete KP) system. The corresponding companion map, which gives generators of the action, is discussed in the generic case, and the corresponding explicit formulas have been found in the periodic reduction. We discuss also briefly connection of the companion to the KP map with context-free languages.
A robust potential-based contact force solution approach for discontinuous deformation analysis of irregular convex polygonal block/particle systems
Authors: Zheng Fei , Zhuang Xiaoying , Zheng Hong , Jiao Yu-Yong , Rabczuk Timon 
Published: July 2020

AbstractContact interaction of two bodies can be modeled using the penalty function approach while its accuracy and robustness are directly associated with the geometry of contact bodies. Particularly, in the research fields of rock mechanics, we need to treat polygonal shapes such as mineral grains/particles at a mesoscale and rock blocks at a macroscale. The irregular shapes (e.g., polygons with small angles or small edges) pose challenges to traditional contact solution approach in terms of algorithmic robustness and complexity. This paper proposed a robust potential-based penalty function approach to solve contact of polygonal particles/block. An improved potential function is proposed considering irregular polygonal shapes. A contact detection procedure based on the entrance block concept is presented, followed by a numerical integral algorithm to compute the contact force. The proposed contact detection approach is implemented into discontinuous deformation analysis with an explicit formulation. The accuracy and robustness of the proposed contact detection approach are verified by benchmarking examples. The potential of the proposed approach in analysis of kinetic behavior of complex polygonal block systems is shown by two application examples. It can be applied in any discontinuous computation models using stepwise contact force-based solution procedures.
The role of heads of departments in the commercialization of university research
Authors: Leitner Karl-Heinz , Bergner Sabine , Rybnicek Robert 
Published: July 2020

AbstractThe commercialization of knowledge has become increasingly important for universities worldwide. Today, the acquisition of third-party funds, the filing of patents and the foundation of spin-offs are seen as similarly relevant to other academic activities such as conducting research and teaching. Research has examined how universities can be supported in their transformation into entrepreneurial institutions and shown that the business experience of scientists might foster the commercialization of knowledge. However, research into the role of academic leaders in this process is scant. In this study, we examine how far the business and scientific experience of the head of department impacts the entrepreneurial activities of an entire department team. Our results demonstrate that heads with business experiences positively influence the acquisition of third-party funds and the patent output of the whole department. However, we found that the foundation of spin-offs is negatively affected by these experiences. Our findings have important policy implications and suggest that business experiences should be considered in the assignment of new heads when reorienting universities into entrepreneurial institutions and restructuring university funding. Yet, the business experience of academic leaders is not a panacea and has its limitations.
Helium droplet assisted synthesis of plasmonic Ag@ZnO core@shell nanoparticles
Authors: Schiffmann Alexander , Jauk Thomas , Knez Daniel , Fitzek Harald , Hofer Ferdinand , Lackner Florian , Ernst Wolfgang E. 
Published: July 2020

AbstractPlasmonic Ag@ZnO core@shell nanoparticles are formed by synthesis inside helium droplets with subsequent deposition and controlled oxidation. The particle size and shape can be controlled from spherical sub-10 nm particles to larger elongated structures. An advantage of the method is the complete absence of solvents, precursors, and other chemical agents. The obtained particle morphology and elemental composition have been analyzed by scanning transmission electron microscopy (STEM) and energy dispersive X-ray spectroscopy (EDS). The results reveal that the produced particles form a closed and homogeneous ZnO layer around a 2–3 nm Ag core with a uniform thickness of (1.33 ± 0.15) nm and (1.63 ± 0.31) nm for spherical and wire-like particles, respectively. The results are supported by ultraviolet photoelectron spectroscopy (UPS), which indicates a fully oxidized shell layer for the particles studied by STEM. The plasmonic properties of the produced spherical Ag@ZnO core@shell particles are investigated by two-photon photoelectron (2PPE) spectroscopy. Upon excitation of the localized surface plasmon resonance in Ag at around 3 eV, plasmonic enhancement leads to the liberation of electrons with high kinetic energy. This is observed for both Ag and Ag@ZnO particles, showing that even if a Ag cluster is covered by the ZnO layer, a plasmonic enhancement can be observed by photoelectron spectroscopy.
ddRAD-seq derived genome-wide SNPs, high density linkage map and QTLs for fruit quality traits in strawberry (Fragaria x ananassa)
Authors: Natarajan Sathishkumar , Hossain Mohammad Rashed , Kim Hoy-Taek , Denison Michael Immanuel Jesse , Ferdous Mostari Jahan , Jung Hee-Jeong , Park Jong-In , Nou Ill-Sup 
Published: July 2020

AbstractUnderstanding the genetic determinants are essential for improving the fruit quality traits of strawberry. In this study, we focused on mapping the loci for fruit-length (FL), -diameter (FD), -weight (FW) and -soluble solid content (SSC) using the genome-wide single nucleotide polymorphisms (SNPs) identified via ddRAD-sequencing of the F1 population raised from Maehyang (♀) X Festival (♂). A total of 12,698 high quality SNPs were identified of which 1554 SNPs that showed significant Mendelian segregation (p < 0.05) were mapped to 53 linkage groups (LG) spanning a total of 2937.93 cM with an average marker density of 2.14 cM/locus. Six QTLs for FL and four QTLs for each of FD, FW and SSC were identified that explained 24–35%, 21–42%, 24–54% and 23–50% of overall phenotypic variations, respectively. The genes that lie within these QTL regions were extracted and discussed thoroughly. In addition, a high resolution melting marker (MF154) were designed based on the SNP A1723G of the UDP-glucose 4-epimerase GEPI48-like gene FAN_iscf00021287. The marker detected the high vs low sugar containing F1 plants and commercial cultivars with 81.39% and 86.95% detection accuracy, respectively. These SNPs, linkage map, QTLs and candidate genes will be helpful in understanding and improving the fruit quality traits of strawberry.
Spectral Demodulation System Based on Two Transmission Volume Bragg Gratings
Authors: Sui Guanghui , Wang Zhiqi , Wang Ning , Huang Peijian , Zhu Yong , Zhang Jie 
Published: July 2020

AbstractIn this paper, a fiber grating demodulation system based on two transmission volume Bragg gratings (VBGs) was proposed. In order to resolve the problem that the spectral resolution of the present fiber grating demodulation system is not high enough, the two transmission VBGs were applied to improve the spectral resolution and reduce the volume of the spectrometer. The diffraction characteristics of the transmission VBGs were analyzed, and the optical path of the two transmission VBGs demodulation system was designed based on the diffraction characteristics. The grating constant, lens parameters, and aberration correction of the system were analyzed and calculated. The calculation showed that the theoretical wavelength range of the demodulation system was from 1525 nm to 1565 nm and the theoretical optical resolution was 60 pm when the grating constant was 0.9168, the angle between two transmission VBGs was 89°, the focal length of the collimator was 60 mm, and the focal length of the imaging lens was 131.5 mm. The aberration of the system was well corrected by using a lens as the collimator and a reflector as the imaging lens. The system principle prototype was assembled and calibrated, and its performances were experimentally investigated. The results showed that the spectrometer worked stably, with a wavelength range from 1525 nm to 1565 nm, an optical wavelength resolution of 65.3 pm, and a high demodulation speed of 10 kHz.