bluuebunny/crossref_metadata_2025_split huggingface dataset:
Potentiation of glutamatergic synaptic transmission onto lateral habenula neurons following early life stress and intravenous morphine self-administration in rats
Authors: Langlois Ludovic D. , Berman Rina Y. , Shepard Ryan D. , Simmons Sarah C. , Tsuda Mumeko C. , Gouty Shawn , Choi Kwang H. , Nugent Fereshteh S. 
Published: December 2020

AbstractEarly life stress (ELS) presents an important risk factor for drug addiction and comorbid depression and anxiety through persistent effects on the mesolimbic dopamine (DA) pathways1. Using an ELS model for child neglect (a single 24 h episode of maternal deprivation, MD) in rats, recent published works from our lab show that MD induces dysfunction in ventral tegmental area (VTA) DA neurons2–4and its negative controller, the lateral habenula (LHb)5–7. In regard to LHb, MD-induced potentiation of glutamatergic synaptic transmission onto LHb neurons shifts the coordination of excitation/inhibition (E/I) balance towards excitation, resulting in an increase in the overall spontaneous neuronal activity with elevation in bursting and tonic firing, and intrinsic excitability of LHb neurons in early adolescent male rats5–7. Here, we explored how MD affects intravenous morphine self-administration (MSA) acquisition and sucrose preference as well as glutamatergic synaptic function in LHb neurons of adult male rats self-administering morphine. We found that MD-induced increases in LHb neuronal and glutamatergic synaptic activity and E/I ratio persisted into adulthood. Moreover, MD significantly reduced morphine intake, triggered anhedonia-like behavior in the sucrose preference test (SPT), and was associated with persistent glutamatergic potentiation 24h after the last MSA session. MSA also triggered postsynaptic glutamatergic potentiation in LHb neurons of control rats during this time period. Our data highlights that ELS-induced glutamatergic plasticity in LHb may dampen the positive reinforcing properties of natural rewards and opioids, and contribute to the development of anhedonic and dysphoric states associated with opioids.
Translatome-based classification reveals a dual metabolic dependency of a new tumor subtype of pancreatic cancer
Authors: Shin Sauyeun , Nicolle Remy , Jean Christine , Samain Remi , Ayadi Mira , Raffenne Jerome , Brunel Alexia , Solorzano Jacobo , Neuzillet Cindy , Joffre Carine , Rocchi Stephane , Iovanna Juan , Dusetti Nelson , Larsson Ola , Pyronnet Stephane , Bousquet Corinne , Martineau Yvan 
Published: December 2020

ABSTRACTMolecular profiling of Pancreatic Ductal Adenocarcinoma (PDA), based on transcriptomic analyses, identifies two main prognostic subtypes (basal-like and classical), but does not allow personalized first-line treatment. To date, tumors have not been profiled based on protein synthesis rates, yet the step of mRNA translation is highly deregulated in both PDA cancer cells and their microenvironment. Using a collection of twenty-seven pancreatic Patient-Derived Xenografts (PDX), we performed genome-wide analysis of translated mRNA (translatome). Unsupervised bioinformatics analysis revealed a new tumor subtype harboring a low protein synthesis rate, but associated with a robust translation of mRNAs encoding effectors of the integrated stress response (ISR), including the transcription factor ATF4. Functional characterization of the “ISR-activated” human cancer cells revealed a high resistance to drugs, low autophagic capacities, and importantly, metabolic impairments in the serine synthesis and transsulfuration pathways. Overall, our study highlights the strength of translatomic profiling on PDA, which here revealed an unforeseen drug-resistant cancer cell phenotype, whose auxotrophy to both serine and cysteine may be amenable to targeted therapy.
Exercise training in a color-polymorphic lizard reveals differential effects of mating tactics and color morphs on telomere, body condition and growth dynamics
Authors: Friesen Christopher R , Wilson Mark , Rollings Nicky , Sudyka Joanna , Giraudeau Mathieu , Whittington Camilla M , Olsson Mats 
Published: December 2020

AbstractAlternative reproductive tactics (ARTs) are correlated suites of sexually selected traits that are likely to impose differential physiological costs on different individuals. While some level of activity might be beneficial, animals living in the wild are often working at the margins of their resources and performance limits. Individuals using ARTs may have divergent capacities for activity, and when pushed beyond their capacity, they may experience condition loss, oxidative stress, and molecular damage that must be repaired with limited resources. We used the Australian painted dragon lizard that exhibits color-polymorphims with corresponding alternative reproductive tactics (ARTs) as a model to experimentally test the effect of exercise on body condition, growth, reactive oxygen species (ROS), and telomere dynamics—a potential marker of stress and aging and a correlate of longevity. For most males, ROS tended to be lower with greater exercise; however, males with yellow throat patches—or bibs— had higher ROS than non-bibbed males. At the highest level of exercise, bibbed males exhibited telomere loss, while non-bibbed males gained telomere length; the opposite pattern was observed in the no-exercise controls. Growth was positively related to food intake but negatively correlated with telomere length at the end of the experiment. Body condition was not related to food intake but was positively correlated with increases in telomere length. These results, along with our previous work, suggest that aggressive bibbed males suffer physiological costs that may reduce longevity.
Reconstructing the human genetic history of mainland Southeast Asia: insights from genome-wide data from Thailand and Laos
Authors: Kutanan Wibhu , Liu Dang , Kampuansai Jatupol , Srikummool Metawee , Srithawong Suparat , Shoocongdej Rasmi , Sangkhano Sukrit , Ruangchai Sukhum , Pittayaporn Pittayawat , Arias Leonardo , Stoneking Mark 
Published: December 2020

AbstractThailand and Laos, located in the center of Mainland Southeast Asia (MSEA), harbor diverse ethnolinguistic groups encompassing all five language families of MSEA: Tai-Kadai (TK), Austroasiatic (AA), Sino-Tibetan (ST), Hmong-Mien (HM) and Austronesian (AN). Previous genetic studies of Thai/Lao populations have focused almost exclusively on uniparental markers and there is a paucity of genome-wide studies. We therefore generated genome-wide SNP data for 33 ethnolinguistic groups, belonging to the five MSEA language families from Thailand and Laos, and analysed these together with data from modern Asian populations and SEA ancient samples. Overall, we find genetic structure according to language family, albeit with heterogeneity in the AA-, HM- and ST-speaking groups, and in the hill tribes, that reflects both population interactions and genetic drift. For the TK speaking groups, we find localized genetic structure that is driven by different levels of interaction with other groups in the same geographic region. Several Thai groups exhibit admixture from South Asia, which we date to ∼600-1000 years ago, corresponding to a time of intensive international trade networks that had a major cultural impact on Thailand. An AN group from Southern Thailand shows both South Asian admixture as well as overall affinities with AA-speaking groups in the region, suggesting an impact of cultural diffusion. Overall, we provide the first detailed insights into the genetic profiles of Thai/Lao ethnolinguistic groups, which should be helpful for reconstructing human genetic history in MSEA and selecting populations for participation in ongoing whole genome sequence and biomedical studies.
A novel mode of photoprotection mediated by a cysteine residue in the chlorophyll protein IsiA
Authors: Chen Hui-Yuan Steven , Niedzwiedzki Dariusz M. , Bandyopadhyay Anindita , Biswas Sandeep , Pakrasi Himadri B. 
Published: December 2020

AbstractOxygenic photosynthetic organisms have evolved multitude mechanisms for protection against high light stress. IsiA, a chlorophylla-binding cyanobacterial protein serves as an accessory antenna complex for photosystem I. Intriguingly, IsiA can also function as an independent pigment protein complex in the thylakoid membrane and facilitate dissipation of excess energy, providing photoprotection. The molecular basis of IsiA-mediated excitation quenching mechanism remains poorly understood. In this study, we demonstrate that IsiA uses a novel cysteine-mediated process to quench excitation energy. The single cysteine in IsiA in the cyanobacteriumSynechocystis6803 was converted to a valine. Ultrafast fluorescence spectroscopic analysis showed that this single change abolishes the excitation energy quenching ability of IsiA, thus providing direct evidence of the crucial role of this cysteine residue in the energy dissipation from excited chlorophylls. Under stress condition, the mutant cells exhibited enhanced light sensitivity, indicating that the cysteine-mediated quenching process is critically important for photoprotection.
Metaproteomic Profiling of Fungal Gut Colonization in Gnotobiotic Mice
Authors: Pettersen Veronika Kuchařová , Dufour Antoine , Arrieta Marie-Claire 
Published: December 2020

ABSTRACTEukaryotic microbes can modulate mammalian host health and disease states, yet the molecular contribution of gut fungi remains nascent. We previously showed that mice exclusively colonized with fungi displayed increased sensitivity to allergic airway inflammation and fecal metabolite profiles similar to germ-free mice. To gain insights into the functional changes attributed to fungal colonization, we performed quantitative proteomic analyses of feces and small intestine of four-week-old gnotobiotic mice colonized with bacteria, fungi, or both. A comparison of fecal metaproteomic profiles between the mouse groups yielded broad changes in the relative levels of bacterial (46% of 2,860) and mouse (76% from 405) proteins. Many of the detected fungal proteins (3% of 1,492) have been previously reported as part of extracellular vesicles and having immunomodulating properties. Changes in the levels of mouse proteins derived from the jejunum (4% of 1,514) were mainly driven by proteins functional in lipid metabolism and apoptosis. Using metaproteomic profiling of gnotobiotic conditions, we show that fungal colonization profoundly impacts the host gut proteome. Our results suggest that an increased abundance of certain gut fungal species in early life may impact the developing intracellular balance of epithelial and immune cells.
Food purchasing behaviour at automatic vending machines: the role of planograms and shopping time
Authors: Marinelli Luca , Fiano Fabio , Gregori Gian Luca , Daniele Lucia Michela 
Published: December 2020

PurposeThe purpose of this paper is to investigate the food and beverage automatic retail environment by analysing the impact of planograms, conceived as a visual merchandising practice and shopping time – the time spent making a purchase – as part of food consumer purchasing behaviour to further enrich the debate on the ability of companies to absorb customer knowledge.Design/methodology/approachA real-world experiment was conducted using a sample of 27,230 valid observations of consumer purchasing decision-making processes at automatic vending machines (AVMs). Data were collected by a shopper behaviour analytics system that allows for a better understanding of the AVM users' behaviour. Two sets of regressions were run to test the two hypotheses.FindingsThe experimental results demonstrated that planograms – the planned, systematic organisation of products in an AVM – positively impact food purchases. A planogram acts as a mediator in the relationship between shopping time and purchase, resulting in shorter shopping times and more purchases.Originality/valueThis work adds to the customer knowledge literature by focussing on customer behaviour in the food and beverage automated shopping environment. The shopper analytics technology adopted to collect real-time data leads to a better understanding of the purchasing behaviour of AVMs' users and provides new marketing and retail insights into AVMs' performance that retailers can use to improve their marketing strategies.
Impact of purchasing practices, supplier relationships and use of information technology on firm performance
Authors: Ambekar Suhas Suresh , Deshmukh Umesh , Hudnurkar Manoj 
Published: December 2020

Purpose
The study aims to establish an impact of supplier relationship and information and communication technology through purchasing practices on firm performance.

Design/methodology/approach
Review of relevant literature resulted in constructs, namely, supplier relationships, information and communication technology, purchasing practices and firm performance. A survey of 179 manufacturing companies through structured questionnaire was conducted. The responses were analysed through structural equation modelling using the partial least squares method.

Findings
It is observed that the firm performance is directly influenced by purchasing practices and indirectly by supplier relationships and information technology. The use of information technology in materials management affects supplier relationships and purchasing practices both.

Practical implications
The study provides a model for purchasing practitioners by highlighting the importance of supplier relationship management. Though the firms are running after improving technology, it can only affect firm performance through proper purchasing practices.

Originality/value
The study provides empirical evidence to the practical notions that exist in purchasing practitioners.
Experimental dynamic analysis of rod-fastening rotor bearing system with a transverse crack
Authors: Wang Nanshan , Liu Heng , Wang Qidan , Qi Shemiao , Liu Yi 
Published: December 2020

PurposeThis study aims to obtain the dynamic behaviours of cracked rod-fastening rotor bearing system (RFBS), and experimental investigation was carried out to examine the dynamic characteristics of this kind of assembled rotor bearing system with a transverse crack passing through the critical speed.Design/methodology/approachAn experimental test rig of cracked RFBS was established for examining the vibration behaviours between intact and cracked system. The crack on the surface of a fastening rod was simulated by wire-electrode cutting processing method. The comprehensive analysis method of vibration was used to obtain the dynamic characteristics such as vibration amplitude, acceleration and whirling orbits before and after the critical speed as well as the instantaneous response in the process of speed up.FindingsSome experimental vibration datum is obtained for cracked RFBS. The appearance of a crack will introduce the initial bending and make the vibration amplitude, acceleration and instant response in the process of speed up increase greatly as well as the change of whirling orbits.Originality/valueThe actual vibration characteristics for this complex assembled rotor system with a transverse crack are given passing through the critical speed. It can provide some useful help for monitoring the vibration behaviours of this kind of assembled rotor system as well as the detection of the crack fault.Peer reviewThe peer review history for this article is available at:https://publons.com/publon/10.1108/ILT-07-2020-0260/
A new frontier: alcohol sponsorship activation through esports
Authors: Kelly Sarah J. , Van der Leij Dymphna 
Published: December 2020

PurposeThe purpose of this paper is to examine the impact of alcohol sponsorship-linked advertising through esports upon young gaming audiences and how gaming behaviours affect advertising response.Design/methodology/approachA cross-sectional survey study was employed to examine the prevalence and nature of alcohol advertising in esports, and the impact of esports participation upon young audiences' consumption and preferences concerning alcohol. Survey data were collected from 976 young Australian gamers aged between 16 and 34 years (58.9% male) using online questionnaires.FindingsResults revealed a vulnerability to alcohol sponsorship and advertising among 25 to 34-year-old and heavy gamer cohorts. As predicted, heavy gamers were more receptive to alcohol advertising in terms of awareness, preference and consumption while gaming than casual gamers.Practical implicationsThis research advances theories of consumer behaviour and advertising exposure situated in a new landscape of converging virtual and real experiential marketing. It also provides much-needed evidence to guide marketing strategy to the next-generation audiences and regulation of new and burgeoning digital platforms. Our research also highlights a need for policy to address the burgeoning, largely unregulated nature of online gaming.Originality/valueThis research provides the first empirical evidence of the impacts of alcohol-linked sponsorship in esports upon young playing and streaming audiences. It informs marketing strategy and policy in relation to the rapidly growing, potentially vulnerable online competitive gaming audience.
Brain structure and clinical profile point to neurodevelopmental factors involved in pedophilic disorder
Authors: Abé Christoph , Adebahr Roberth , Liberg Benny , Mannfolk Christian , Lebedev Alexander , Eriksson Jonna , Långström Niklas , Rahm Christoffer 
Published: December 2020

AbstractObjectivePedophilic disorder (PD) is characterized bypersistent, intense sexual attraction to prepubertal children that the individual has acted on, or causes marked distress or interpersonal difficulty. Although prior research suggests that PD has neurodevelopmental underpinnings, the evidence remains sparse. To aid the understanding of etiology and treatment development, we quantified neurobiological and clinical correlates of PD.MethodWe compared 55 self‐referred, help‐seeking, non‐forensic male patients with DSM‐5 PD with 57 age‐matched, healthy male controls (HC) on clinical, neuropsychological, and structural brain imaging measures (cortical thickness and surface area, subcortical and white matter volumes). Structural brain measures were related to markers for aberrant neurodevelopment including IQ, and the 2nd to 4th digit ratio (2D:4D).ResultsPD was associated with psychiatric disorder comorbidity and ADHD and autism spectrum disorder symptoms. PD patients had lower total IQ than HC. PD individuals exhibited cortical surface area abnormalities in regions belonging to the brain's default mode network and showed abnormal volume of white matter underlying those regions. PD subjects had smaller hippocampi and nuclei accumbens than HC. Findings were not related to history of child‐related sexual offending. IQ correlated negatively with global expression of PD‐related brain features and 2D:4D correlated with surface area in PD.ConclusionsIn the largest single‐center study to date, we delineate psychiatric comorbidity, neurobiological and cognitive correlates of PD. Our morphometric findings, their associations with markers of aberrant neurodevelopment, and psychiatric comorbidities suggest that neurodevelopmental mechanisms are involved in PD. The findings may need consideration in future development of clinical management of PD patients.
Axial skeleton morphology of the Western Palearctic aphaniid fishes (Teleostei: Cyprinodontiformes; Family: Aphaniidae)
Authors: Teimori Azad , Esmaeili Hamid Reza 
Published: December 2020

AbstractThe axial skeleton morphology of eighteen aphaniid species was examined for the first time by X‐ray radiography, a non‐lethal method. The number of total vertebrae in the genus Aphanius is ranged from 26 to 29, in Aphaniops, it is ranged from 24 to 28, and in Paraphanius mento, it is ranged from 26 to 27. The vertebral column in Aphanius was often slightly curved, and it is varied from almost straight (most common) to slightly or strongly curved and straight in two others. The caudal skeleton of the family Aphaniidae is characterized by a single and completely fused hypural plate, and a total number of 9–12 branched segmented soft rays, a single and elongate epural bone. All examined species of the genus Aphanius and Paraphanius mento displayed a straight epural bone in their caudal skeleton, while it was often sinuous‐like and thick in all species of the genus Aphaniops. This study supports the assumption that a sinuous epural is likely the first detected morphological synapomorphy for the “inner” clade of Aphaniops. The family Aphaniidae is closer to Cyprinodontidae, Valenciidae and Fundulidae than other cyprinodontiforms in the caudal skeleton, which is in agreement with the multigene phylogeny of cyprinodontiforms.
Hydrogen sulfide donors prevent lipopolysaccharide‐induced airway hyperreactivity in an in vitro model of chronic inflammation in mice
Authors: Karaman Yasemin , Kaya‐Yasar Yesim , Bozkurt T Emrah , Sahin‐Erdemli Inci 
Published: December 2020

AbstractWe aimed to investigate and compare the effects of rapid (NaHS) and slow (GYY4137 and AP39) hydrogen sulfide (H2S) releasing donors on LPS‐induced tracheal hyperreactivity and pro‐inflammatory cytokine levels in lung tissues of mice. Tissues were isolated from male BALB/c mice and incubated with LPS (10 µg/mL) in tissue culture. The subgroups were incubated with NaHS, GYY4137 and mitochondria‐targeted donor AP39. LPS incubation did not alter contraction response to carbachol, but enhanced 5‐HT and bradykinin‐induced contractions in tracheal rings, and elevated IL‐1β, IL‐6 and TNF‐α levels in lung homogenates. NaHS at 300 µmol/L and 1000 µmol/L, GYY4137 at 30 µmol/L and 100 µmol/L, and AP39 at 30 nmol/L concentrations inhibited the tracheal hyperreactivity to 5‐HT, whereas none of these donors affected the enhanced contraction to bradykinin. GYY4137 was also effective to inhibit 5‐HT hyperreactivity acutely. In lung tissues, NaHS prevented the elevation of IL‐1β level at 1000 μmol/L, and IL‐6 and TNF‐α levels at 100 μmol/L concentrations. Incubation with GYY4137 (100 µmol/L) and AP39 (30 nmol/L and 300 nmol/L) inhibited the increase in IL‐6 and TNF‐α levels, but not IL‐1β at concentrations that they affected tracheal hyperreactivity. These results indicate that H2S donors can decrease inflammation and prevent airway hyperreactivity.
N‐acetylcysteine as an adjunctive treatment for bipolar depression: A systematic review and meta‐analysis of randomized controlled trials
Authors: Nery Fabiano G. , Li Wenbin , DelBello Melissa P. , Welge Jeffrey A. 
Published: December 2020

AbstractObjectivesPrevious studies and meta‐analyses suggested that N‐acetylcysteine (NAC) was superior to placebo in improving depression in bipolar disorder. However, more recent data, including two larger trials, found that NAC was no more effective than placebo. We conducted a meta‐analysis to appraise the possible efficacy of NAC in treating bipolar depression.MethodsA systematic review and meta‐analysis of double‐blind, placebo‐controlled trials of NAC as a treatment augmentation strategy for bipolar depression was carried out in PubMed (1966–2020). We utilized random‐effect analysis to evaluate improvement in depressive symptoms from baseline to endpoint as the primary efficacy measure.ResultsSix trials including 248 patients were included. Treatment augmentation with NAC showed a moderate effect size favoring NAC over placebo (d = 0.45, 95% C.I.: 0.06–0.84). There was substantial heterogeneity (I2 = 49%). Meta‐regression analyses did not identify any moderator that might explain variation in heterogeneity, including baseline depressive symptom scores, mean NAC dose, or duration of study.ConclusionsResults from six clinical trials suggest that treatment augmentation with NAC for bipolar depression appears to be superior to placebo, with a moderate effect size, but a large confidence interval. Larger clinical trials, investigating possible moderating factors, such as NAC dose, treatment duration, baseline depression severity, or chronicity of illness, are warranted.