bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cardiomyocyte-Specific JunD Overexpression Increases Infarct Size following Ischemia/Reperfusion Cardiac Injury by Downregulating Sirt3
Authors: Akhmedov Alexander , Montecucco Fabrizio , Costantino Sarah , Vdovenko Daria , Schaub Clerigué Ariane , Gaul Daniel S. , Burger Fabienne , Roth Aline , Carbone Federico , Liberale Luca , Amrollahi-Sharifabadi Mohammad , Vellone Valerio Gaetano , Eriksson Urs , Matter Christian M. , Crowe Lindsey A. , Vallée Jean-Paul , Paneni Francesco , Vanhoutte Paul M. , Camici Giovanni G. , Mach François , Lüscher Thomas F. 
Published: December 2019

AbstractIschemia/reperfusion (I/R) injury in acute myocardial infarction activates several deleterious molecular mechanisms. The transcription factor JunD regulates pathways involved in oxidative stress as well as in cellular proliferation, differentiation, and death. The present study investigated the potential role of JunD as a modulator of myocardial injury pathways in a mouse model of cardiac I/R injury. Infarct size, systemic and local inflammation, and production of reactive oxygen species, as well as cytosolic and mitochondrial apoptotic pathways were investigated in adult males after myocardial I/R. In wild-type (WT) mice, 30 minutes after ischemia and up to 24 hours following reperfusion, cardiac JunD messenger ribonucleic acid expression was reduced while JunB increased. Cardiac-specific JunD overexpressing mice (JunDTg/0
) displayed larger infarcts compared with WT. However, postischemic inflammatory or oxidative responses did not differ. JunD overexpression reduced Sirt3 transcription by binding to its promoter, thus leading to mitochondrial dysfunction, myocardial cell death, and increased infarct size. On the other hand, JunD silencing reduced, while Sirt3 silencing increased infarct size. In human myocardial autopsy specimens, JunD-positive areas within the infarcted left ventricle staining corresponded to undetectable Sirt3 areas in consecutive sections of the same heart. Cardiac-specific JunD overexpression increases myocardial infarct size following I/R. These effects are mediated via Sirt3 transcriptional repression, mitochondrial swelling, and increased apoptosis, suggesting that JunD is a key regulator of myocardial I/R injury. The present data set the stage for further investigation of the potential role of Sirt3 activation as a novel target for the treatment of acute myocardial infarction.
Particulate Shiga Toxin 2 in Blood is Associated to the Development of Hemolytic Uremic Syndrome in Children
Authors: Brigotti Maurizio , He Xiaohua , Carnicelli Domenica , Arfilli Valentina , Porcellini Elisa , Galassi Elisabetta , Tazzari Pier Luigi , Ricci Francesca , Patfield Stephanie A. , Testa Sara , Paglialonga Fabio , Picicco Damiano , Caprioli Alfredo , Scavia Gaia , Morabito Stefano , Ardissino Gianluigi 
Published: December 2019

AbstractHemolytic uremic syndrome (HUS), the leading cause of acute renal failure in children (< 3 years), is mainly related to Shiga toxins (Stx)-producing Escherichia coli (STEC) infections. STEC are confined to the gut resulting in hemorrhagic colitis, whereas Stx are delivered in blood to target kidney and brain, with unclear mechanisms, triggering HUS in 5 to 15% of infected children. Stx were found on circulating cells, free in sera (soluble Stx) or in blood cell-derived microvesicles (particulate Stx), whereby the relationship between these forms of circulating toxins is unclear. Here, we have examined 2,846 children with bloody diarrhea and found evidence of STEC infection in 5%. Twenty patients were enrolled to study the natural course of STEC infections before the onset of HUS. In patients, Stx were found to be associated to circulating cells and/or free and functionally active in sera. In most children, Stx were bound to neutrophils when high amounts of toxins were found in feces. Time-course analysis showed that Stx increased transiently in patients' sera while the decrease of toxin amount on leukocytes was observed. Notably, patients who recovered (85%) displayed different settings than those who developed HUS (15%). The distinctive feature of the latter group was the presence in blood of particulate Stx2 (Stx2 sedimented at g-forces corresponding to 1 μm microvesicles) the day before diagnosis of HUS, during the release phase of toxins from circulating cells. This observation strongly suggests the involvement of blood cell-derived particulate Stx2 in the transition from hemorrhagic colitis to HUS.
Hypoxia Modulates Platelet Purinergic Signalling Pathways
Authors: Paterson Gordon G. , Young Jason M. , Willson Joseph A. , Graham Christopher J. , Dru Rebecca C. , Lee Eleanor W. , Torpey Greig S. , Walmsley Sarah R. , Chan Melissa V. , Warner Timothy D. , Baillie John Kenneth , Thompson Alfred Arthur Roger 
Published: December 2019

Abstract
Background Hypoxia resulting from ascent to high-altitude or pathological states at sea level is known to increase platelet reactivity. Previous work from our group has suggested that this may be adenosine diphosphate (ADP)-specific. Given the clinical importance of drugs targeting ADP pathways, research into the impact of hypoxia on platelet ADP pathways is highly important.
Methods Optimul aggregometry was performed on plasma from 29 lowland residents ascending to 4,700 m, allowing systematic assessment of platelet reactivity in response to several platelet agonists. Aggregometry was also performed in response to ADP in the presence of inhibitors of the two main ADP receptors, P2Y1 and P2Y12 (MRS2500 and cangrelor, respectively). Phosphorylation of vasodilator-stimulated phosphoprotein (VASP), a key determinant of platelet aggregation, was analysed using the VASPFix assay.
Results Hypobaric hypoxia significantly reduced the ability of a fixed concentration of cangrelor to inhibit ADP-induced aggregation and increased basal VASP phosphorylation. However, in the absence of P2Y receptor inhibitors, we did not find evidence of increased platelet sensitivity to any of the agonists tested and found reduced sensitivity to thrombin receptor-activating peptide-6 amide.
Conclusion Our results provide evidence of increased P2Y1 receptor activity at high altitude and suggest down-regulation of the P2Y12 pathway through increased VASP phosphorylation. These changes in ADP pathway activity are of potential therapeutic significance to high-altitude sojourners and hypoxic sea level patients prescribed platelet inhibitors and warrant further investigation.
Medial Patellofemoral Ligament Reconstruction and Nonanatomic Stabilization Techniques in Skeletally Immature Patients
Authors: Pilone Carola , Bonasia Davide Edoardo , Rosso Federica , Cottino Umberto , Mazzola Claudio , Blonna Davide , Rossi Roberto ,   Sigascot Knee Committee
Published: December 2019

AbstractPatellar instability is a common cause of knee disability in children and adolescent, with a high recurrence rate. When conservative treatment fails, surgical options should be considered. The femoral insertion of the medial patellofemoral ligament (MPFL) is in close proximity to the distal femoral growth plate and precautions should be taken to avoid injuries to the physis. Anatomical features of the MPFL complex, with focus on the relationship between femoral MPFL attachment and femoral physis, are discussed together with surgical tips to avoid injuries to the growth plates. The aim of this article is to review the recent literature regarding MPFL reconstruction and other stabilization techniques for patellofemoral instability in skeletally immature patients, focusing on the different surgical options available. These can be classified as anatomical versus nonanatomical, proximal versus distal realignments, or based on the graft used: free graft and pedicled graft (quadriceps, patellar tendon, hamstring, and adductor magnus).
Mini Bone Block Distraction Subtalar Arthrodesis (SAMBB) in the Management of Acquired Adult Flatfoot with Subtalar Arthritis: A Modification to the Grice–Green Procedure
Authors: Mosca Massimiliano , Caravelli Silvio , Vannini Francesca , Pungetti Camilla , Catanese Giuseppe , Massimi Simone , Fuiano Mario , Faldini Cesare , Giannini Sandro 
Published: December 2019

AbstractAdult acquired flatfoot encompasses a wide range of deformities. The goal of surgical treatment of adult acquired flatfoot is to achieve proper alignment of the hindfoot and maintain as much flexibility as possible in the foot and ankle complex; nevertheless, if subtalar arthritis is present, subtalar arthrodesis is usually mandatory. A lateral approach over the sinus tarsi is performed and subtalar joint is prepared removing remaining cartilage, reduced up to 5 degrees of heel valgus, and stabilized with a Kirschner wire. The site of insertion of the corticocancellous bone graft, harvested from the ipsilateral proximal tibia, is prepared carving a vertical groove into the talar and calcaneal edges. Additional autologous cancellous chips, harvested from the proximal tibia, can be positioned and pressed with a beater into the sinus tarsi to enhance fusion. The technique described is a modification of the extra-articular arthrodesis originally proposed by Grice. mini bone block distraction subtalar arthrodesis is a simple and effective technique which permits a subtalar fusion with a restored orientation of the hindfoot and ankle alignment in acquired flatfoot with subtalar arthritis.
Maternal Hypothyroidism during Pregnancy and the Risk for Infectious Morbidity of the Offspring
Authors: Beharier Ofer , Walfisch Asnat , Wainstock Tamar , Szaingurten-Solodkin Irit , Landau Daniela , Sheiner Eyal 
Published: December 2019

Abstract
Objective Animal studies indicate a possible intrauterine immunological imprinting in pregnancies complicated by hypothyroidism. We aimed to evaluate whether exposure to maternal hypothyroidism during pregnancy increases the risk of long-term infectious morbidity of the offspring.
Study Design A retrospective cohort study compared the long-term risk of hospitalization associated with infectious morbidity in children exposed and unexposed in utero to maternal hypothyroidism. Outcome measures included infectious diagnoses obtained during any hospitalization of the offspring (up to the age of 18 years).
Results The study included 224,950 deliveries. Of them, 1.1% (n = 2,481) were diagnosed with maternal hypothyroidism. Children exposed to maternal hypothyroidism had a significantly higher rate of hospitalizations related to infectious morbidity (13.2 vs. 11.2% for control; odds ratio: 1.2; 95% confidence interval: 1.08–1.36; p = 0.002). Specifically, incidences of ear, nose, and throat; respiratory; and ophthalmic infections were significantly higher among the exposed group. The Kaplan–Meier curve indicated that children exposed to maternal hypothyroidism had higher cumulative rates of long-term infectious morbidity. In the Cox proportional hazards model, maternal hypothyroidism remained independently associated with an increased risk of infectious morbidity in the offspring while adjusting for confounders.
Conclusion Maternal hypothyroidism during pregnancy is associated with significant pediatric infectious morbidity of the offspring.
Is Nasal Septum–Tragus Length Measurement Appropriate for Endotracheal Tube Intubation Depth in Neonates? A Randomized Controlled Study
Authors: Uygur Özgün , Öncel Mehmet Yekta , Şimşek Gülsüm Kadıoğlu , Okur Nilüfer , Çelik Kıymet , Bozkurt Özlem , Yücesoy Ebru , Terek Demet , Arslan Meltem Koyuncu , Pekçevik Yeliz , Akar Melek , Köroğlu Özge Altun , Oğuz Suna , Kültürsay Nilgün 
Published: December 2019

AbstractObjective Endotracheal intubation is a frequent procedure performed in neonates with respiratory distress. Clinicians use different methods to estimate the intubation insertion depth, but, unfortunately, the improper insertion results are very high. In this study, we aimed to compare the two different methods (Tochen's formula = weight in kilograms + 6 cm; and nasal septum–tragus length [NTL] + 1 cm) used to determine the endotracheal tube (ETT) insertion depth.Study Design Infants who had intubation indications were enrolled in this study. The intubation tube was fixed using the Tochen formula (Tochen group) or the NTL + 1 cm formula (NTL group). After intubation, the chest radiograph was evaluated (above T1, proper place, and below T2).Results A total of 167 infants (22–42 weeks of gestational age) were included in the study. The proper tube placement rate in both groups was similar (32.4 vs. 30.4% for infants < 34 weeks of gestational age and 56.8 vs. 45.0% in infants > 34 weeks of gestational age). The ETT was frequently placed below T2 at a higher rate in infants with a gestational age of <34 weeks, especially in the NTL group (46% in the Tochen group and 60.7% in the NTL group).Conclusion The NTL + 1 cm formula led to a higher rate of ETT placement below T2, especially in infants with a birth weight of <1,500 g. Therefore, more studies are needed to determine the optimal ETT insertion depth.
References Values of Fetal Heart Myocardial Volume by Three-Dimensional Ultrasound using Spatiotemporal Image Correlation and Virtual Organ Computer-Aided Analysis Methods and Their Applicability in Pregestational Diabetic Women
Authors: Melo Júnior José Francisco , Bravo-valenzuela Nathalie Jeanne , Nardozza Luciano Marcondes Machado , Peixoto Alberto Borges , Mattar Rosiane , Martins Wellington P. , Pares David Baptista da Silva , Araujo Júnior Edward 
Published: December 2019

Abstract
Objective To determine reference values for myocardial volume of the fetal heart using three-dimensional ultrasound with spatiotemporal image correlation (STIC) and virtual organ computer-aided analysis (VOCAL) and to assess their applicability in women with pregestational diabetes.
Study Design This retrospective cross-sectional study included 177 normal pregnant women with fetuses between 200/7 and 336/7 weeks of age. Fetal cardiac volumes were collected using the STIC method, and myocardial volume was obtained by subtraction of the intracavitary volumes using the VOCAL 30-degree method. Intra- and interobserver reproducibility values were determined using the concordance correlation coefficient (CCC). Sixteen women with pregestational diabetes mellitus were evaluated for validation.
Results There was a strong correlation between fetal myocardial volume and gestational age (R
2 = 0.82). Intra- and interobserver reproducibility values were excellent and moderate, respectively, with CCCs of 0.99 and 0.83, respectively. There was no significant difference in mean fetal myocardial volume between normal pregnant women and those with pregestational diabetes (p = 0.64).
Conclusion Reference values for myocardial volume of the fetal heart were determined in normal pregnant women and were not statistically different from those in women with pregestational diabetes mellitus.
Piezosurgery in Hallux Valgus Correction: Distal Linear Osteotomy Operative Technique Using Piezoelectric Tools
Authors: Russo Alessandro , Caravelli Silvio , Mosca Massimiliano , Girolami Mauro , Ortolani Alessandro , Massimi Simone , Fuiano Mario , Zaffagnini Stefano 
Published: December 2019

Abstract
Purpose Piezoelectric and ultrasonic vibrations have been used to cut tissues for three decades, in particular, in periodontics. The increasing use of piezosurgery is based on its clinical advantages such as selective cutting, precision, and low-temperature work rates. The authors applied this concept to a new operative field, the foot and ankle pathology and surgery, such as hallux valgus corrective distal linear osteotomy.
Methods The osteotome equipped was the Surgysonic Moto-II model (Esacrom, Imola, Italy), a system recently developed for cutting bone withmicrovibrations. Tips used in author’s case series were a high-efficiency five teeth piezoelectric saw and a high-efficiency flat scalpel shaped on three edges. Operative technique is described.
Discussion and Conclusion Piezoelectric techniques were developed in response to the need for great precision and safety in bone surgery that was availavle with other manual and rmotorised instruments. Piezo-technology allows minimally-invasive and percutaneous surgery, with reduced trauma on periostium, bone, and soft tissues, reduced healing time of the osteotomy due to the absence of bony necrosis and debris formation and major precision.
Changes in isometric quadriceps strength after the application of ultrasound-guided percutaneous neuromodulation. A case study
Authors: O. Carvajal-Fernández , D. Álvarez Prats , N. Pérez Mallada 
Published: December 2019

Abstract
Background Ultrasound-guided percutaneous neuromodulation (US-guided PNM) is an invasive physiotherapy technique which is employed for neurofunctional improvement and treatment of pain. To date, no study has related this technique with muscle stimulation and dynamometry changes. Within the functional assessment tools, dynamometry tests were performed with devices which enable the calculation of variables related with isometric and isokinetic movement.
Aim To assess the changes in maximum isometric strength after the application of US-guided PNM.
Material and Methods A retrospective case study comprising 13 subjects (26 lower limbs) receiving US-guided PNM, with pre and post-intervention measurements.The inclusion criteria comprised subjects without pain at the time of study, with coefficients of variation (COV) below 15% and in the process of improving loading work. We excluded subjects with pathology provoking pain at the time of the measurements and with contraindications for dynamometry or US-guided PNM. An isometricmeasurement was performed using the KINEO dynamometry system with 90° hip flexion and 45° knee extension, without strapping and with manual grips in lateral supports of the system. The lever arm was placed at 2 cm of the malleoli in the ventral aspect without strapping the ankle and a pre-intervention measurement was performed of the maximum isometric strength based on a mean of 3 repetitions (3 seconds' contraction and 6 seconds' relaxation). Subsequently, the US-guided PNM technique was performed on the femoral nerve, using the Physio Invasiva device and the PES modality (10 Hz frequency, 240 μs pulse width). Ten maximal and pain free stimulations were performed lasting 10 seconds each, with a 10 second rest between each contraction. After the intervention, a post intervention measurement was performed, in the same manner as the previous measurement.
Results In total, 13 volunteer subjects participated in the study, aged between 27 and 59 years, of which, 2 were women and 11 were men. After the application of US-guided PNM on the femoral nerve, changes were observed in the maximum mean isometric strength of the quadriceps, which increased from 26.75 kg in the pre-intervention mean with a standard deviation of 7.42 kg to 30.05 kg for the post-intervention mean, with a standard deviation of 9.23 kg. The results were statistically significant with p < 0.000.
Conclusion US-guided PNM applied to the femoral nerve is able to produce changes in maximum isometric strength measured using dynamometry.
Novel treatment with percutaneus electrolysis in cervical whiplash syndrome
Authors: JR García Naranjo 
Published: December 2019

Introduction and Aims Cervical whiplash syndrome (CWS) commonly occurs because of the rear impact of motor vehicle collisions, producing a forced passive extension of the neck, followed by a rapid flexion in the form of a whip. This violent flexion can lead to an enthesopathy of the levator scapulae muscle (LS). The aim of this study was to evaluate the effectiveness of the percutaneous needle electrolysis (PNE) technique on the enthesopathy of the LS after a CWS and to compare this with a conventional physical therapy protocol.
Material and Methods In a physical therapy center specialized in traffic accidents, we arranged to interview a sample of 100 voluntary patients, providing subjects with the inclusion-exclusion criteria and an informed consent form. Participants recruited to the study had their pain quantified using the visual analog scale (VAS) for pain, the validated neck pain questionnaire (NPQ), and an algometer. Two groups were created. One received the PNE technique and the other received the conventional physiotherapy protocol. Once treatments were completed, pain was quantified once again and the results were obtained.
Results The most important findings are highlighted, regarding the comparison of variables of pain quantification in both groups and regarding the cost per treatment session.
Conclusions
1. The conventional physiotherapy protocol used is effective for the reduction of pain in the SLC in the enthesopathy of the LS.2. Treatment with the PNE technique is effective for the reduction of pain in the CWS for LS enthesopathy.3. Physiotherapy treatment which includes the PNE technique is more effective in the algometry variable than treatment performed with conventional physiotherapy techniques by 16.8%.3. The PNE technique is superior to the protocol of conventional physiotherapy in the cost/effectiveness relationship by 85%.4. The PNE technique, without combining the same with any other physiotherapy technique, is effective for the reduction of pain in cases of enthesopathy.
Invasive techniques in physical therapy: A literature review
Authors: L. Baraja-Vegas , M. Troyano Ruiz , E. Martí Rubio , J. Galvéz García 
Published: December 2019

Abstract
Background Invasive techniques in physical therapy is a term used to refer to a group of techniques in which the physical agent used for the treatment of different pathologies crosses the skin barrier. The aim of this study was to examine the scientific evidence on the invasive techniques that are most used in physical therapy, by reviewing the literature available on this subject.
Material and Methods A literature search was performed on the EBSCOhost, PubMed, Web of Science (WOS), PEDro and TRIP databases. The selected studies were: randomized clinical trials (RCTs), systematic reviews of RCTs and meta-analyses of RCTs. Studies had to be published between 2008 and 2018, conducted on men and women over the age of 18 and in English or Spanish.
Results In total, 64 studies were retrieved, which were read and scrutinized to confirm whether they were relevant for the present study. Finally, after the various screening processes, a sample of 21 articles was obtained. The level of evidence was analyzed as well as the level of recommendation according to the Oxford scale, together with an impact index according to the Journal Citation Reports (JCR). Seventeen of the studies were meta-analyses and systematic reviews of RCTs, and one systematic review of cohort studies was included.
Conclusion After the analysis of the selected articles and the conclusions, invasive techniques in physical therapy are confirmed to be relatively safe and effective for different pathologies, especially when performed in depth and when the local twitch response (LTR) is sought.
Clinical criteria for the application of dry needling in myofascial pain Syndrome: An expert consensus document and a cross-sectional study among physical therapists
Authors: Secorro Nicolas , Guerra Rafael , Labraca Xavi , Lari Marc , Pecos Daniel , Torres Bárbara , Calvo Sandra 
Published: December 2019

AbstractPresently, there is no clear consensus on the essential and confirmatory criteria which should govern the application of dry needling (DN) in the treatment of myofascial trigger points. The aim of this study was to generate a consensus on these criteria, via a panel discussion with DN experts which took place at the International Conference of Invasive Physical Therapy held in 2018, including the opinion of the attendees who participated in a live survey on the subject at the conference via an app. The results obtained reveal discrepancies regarding confirmatory criteria such as the elicitation of referred pain; nonetheless, consensus exists on the suitability of the application of individualized and personalized DN treatment and the combination of treatment with other intervention approaches in physical therapy, with the use of ultrasound when required to support a safer clinical practice.