bluuebunny/crossref_metadata_2025_split huggingface dataset:
Single‐cell transcriptome analysis reveals thyrocyte diversity in the zebrafish thyroid gland
Authors: Gillotay Pierre , Shankar Meghna , Haerlingen Benoit , Eski Sema Elif , Pozo‐Morales Macarena , Garteizgogeascoa Inés , Reinhardt Susanne , Kränkel Annekathrin , Bläsche Juliane , Petzold Andreas , Ninov Nikolay , Kesavan Gokul , Lange Christian , Brand Michael , Lefort Anne , Libert Frédérick , Detours Vincent , Costagliola Sabine , Sumeet Pal Singh 
Published: November 2020

AbstractThe thyroid gland regulates growth and metabolism via production of thyroid hormone in follicles composed of thyrocytes. So far, thyrocytes have been assumed to be a homogenous population. To uncover heterogeneity in the thyrocyte population and molecularly characterize the non‐thyrocyte cells surrounding the follicle, we developed a single‐cell transcriptome atlas of the region containing the zebrafish thyroid gland. The 6249‐cell atlas includes profiles of thyrocytes, blood vessels, lymphatic vessels, immune cells, and fibroblasts. Further, the thyrocytes show expression heterogeneity, including bimodal expression of the transcription factor pax2a. To validate thyrocyte heterogeneity, we generated a CRISPR/Cas9‐based pax2a knock‐in line that monitors pax2a expression in the thyrocytes. A population of pax2a‐low mature thyrocytes interspersed in individual follicles can be distinguished. We corroborate heterogeneity within the thyrocyte population using RNA sequencing of pax2a‐high and pax2a‐low thyrocytes, which demonstrates 20% differential expression in transcriptome between the two subpopulations. Our results identify and validate transcriptional differences within the presumed homogenous thyrocyte population.
Inositol‐requiring enzyme‐1 regulates phosphoinositide signaling lipids and macrophage growth
Authors: Hamid Syed Muhammad , Citir Mevlut , Terzi Erdem Murat , Cimen Ismail , Yildirim Zehra , Dogan Asli Ekin , Kocaturk Begum , Onat Umut Inci , Arditi Moshe , Weber Christian , Traynor‐Kaplan Alexis , Schultz Carsten , Erbay Ebru 
Published: November 2020

AbstractThe ER‐bound kinase/endoribonuclease (RNase), inositol‐requiring enzyme‐1 (IRE1), regulates the phylogenetically most conserved arm of the unfolded protein response (UPR). However, the complex biology and pathology regulated by mammalian IRE1 cannot be fully explained by IRE1’s one known, specific RNA target, X box‐binding protein‐1 (XBP1) or the RNA substrates of IRE1‐dependent RNA degradation (RIDD) activity. Investigating other specific substrates of IRE1 kinase and RNase activities may illuminate how it performs these diverse functions in mammalian cells. We report that macrophage IRE1 plays an unprecedented role in regulating phosphatidylinositide‐derived signaling lipid metabolites and has profound impact on the downstream signaling mediated by the mammalian target of rapamycin (mTOR). This cross‐talk between UPR and mTOR pathways occurs through the unconventional maturation of microRNA (miR) 2137 by IRE1’s RNase activity. Furthermore, phosphatidylinositol (3,4,5) phosphate (PI(3,4,5)P3) 5‐phosphatase‐2 (INPPL1) is a direct target of miR‐2137, which controls PI(3,4,5)P3 levels in macrophages. The modulation of cellular PI(3,4,5)P3/PIP2 ratio and anabolic mTOR signaling by the IRE1‐induced miR‐2137 demonstrates how the ER can provide a critical input into cell growth decisions.
Tezukuri dan Percaya Diri: Representasi Perempuan dalam Iklan Cokelat Lotte Ghana Edisi Valentine
Authors: Pasaribu Rouli Esther , Putri Arnaisya Rachyu 
Published: November 2020

Penelitian ini bertujuan untuk melihat bagaimana perempuan direpresentasikan pada iklan cokelat Lotte Ghana di hari Valentine yang ditayangkan oleh televisi Jepang selama satu dekade terakhir (2010-2019). Penelitian ini menggunakan metode analisis teks dengan teori representasi dari Stuart Hall dan konsep stereotip sifat dan peran gender dari Mary E. Kite. Hasil penelitian menunjukkan bahwa iklan cokelat Lotte Ghana mendukung wacana perempuan ideal yang sesuai dengan standar norma patriarki. Sekalipun perempuan dalam iklan ini memiliki karakteristik maskulin seperti aktif, percaya diri, berani, tetapi semua karakteristik itu tetap dibatasi dalam peran femininnya yang aktif di dapur dan membuat cokelat buatan tangan sendiri untuk laki-laki yang disukai. Hal ini menunjukkan bahwa pada hari Valentine perempuan di Jepang memiliki kuasa serta peran aktif untuk berekspresi, tetapi kuasa yang ditampilkan tetap ada kaitannya dengan menyenangkan hati laki-laki, terbatas hanya pada hubungan laki-laki dan perempuan dan tidak berfokus pada perempuan itu sendiri serta tetap berada di ranah domestik. Selain itu, perempuan dalam iklan juga digunakan sebagai alat untuk menarik pembeli cokelat demi meraup keuntungan besar, sehingga dapat disimpulkan pada pembacaan di level yang lebih dalam, iklan Lotte Ghana merupakan wujud kelindan antara wacana patriarki dan kapitalisme.
Purwarupa dan Kinerja Pengering Gabah Hybrid Solar Heating dan Photovoltaic Heater dengan Sistem Monitoring Suhu
Authors: Usman Usman , Muchtar Akhyar , Muhammad Umar , Lestari Nunik 
Published: November 2020

Grain drying is a process to reduce grain moisture content to certain conditions, so the grain can last longer in storage. The grain dryer model used in this research was Indirect Type Solar Dryer (ITSD). In order to make this dryer can work at night, heating element that used electrical energy from solar panels was added. This energy is a renewable and environmentally friendly energy. This dryer was equipped with a temperature monitoring system and control of the photovoltaic heater. The results of temperature monitoring and voltage sensors to controlling photovoltaic heaters based on validation are categorized as work measurement tools, because they have an error of 0.5% – 2%. Whereas the relay works when the battery voltage is 11 - 10.9 V. This dryer can dry grain to reach a moisture content of 14.90% from initial moisture content of 48.46%. The drying process lasts for 11 hours, which is 7 hours using solar energy and 4 hours using photovoltaic heaters. The average temperature produced by dryer system during the drying process is 35.28 °C with a drying efficiency of 60.14%.
PENGEMBANGAN INSTRUMEN PENILAIAN MENDENGARKAN SANDIWARA BAHASA JAWA SMP KELAS IX BERBASIS KULTURAL DI KOTA SEMARANG
Authors: Prastya Dian 
Published: November 2020

Abstract
This study is based on the fact that the listening rating achievments of the school play have not in accordance with literary discourse listening test. Usually, the teacher only gives brief description of the play. Therefore, the aim of this study is develop cultural based of listening instrument in thetrical subject. This reseach uses resesarch and development design. Reseach data were obtained from interviews and valid test questionnaires. The instrument produced according to the eeds of the teacher, the instrument of cultural-based theatrical play which contains the recording of the plays of Prince Mangkubumi (Pandanaran II) and nine forms of listening to the literally discourse. Expert states that the instruments and recording of plays are valid with revisions.
Keywords: listening plays, assessment instruments.

Abstrak
Penelitian ini didasari pada kenyataan bahwa penilaian mendengarkan sandiwara di sekolah belum sesuai dengan keterampilan mendengarkan wacana sastra. Oleh karena itu, penelitian ini mengembangkan instrumen penilaian mendengarkan sandiwara berbasis kultural. Penelitian ini menggunakan desain Research and Development. Data penelitian diperoleh dari wawancara dan angket uji validasi. Instrumen yang dihasilkan sesuai dengan kebutuhan guru, yaitu instrumen penialaian sandiwara berbasis kultural yang berisikan rekamana sandiwara Pangeran Mangkubumi (Pandanaran II) dan sembilan bentuk soal mendengarkan wacana sastra.Hasil uji ahli menyatakan bahwa instrumen dan rekaman sandiwara valid dengan revisi.
Kata kunci: mendengarkan sandiwara, instrumen penilaian.
Pengembangan Buku Cerita Bergambar Berbasis Serat Wulangreh Pupuh Pangkur untuk Pembelajaran Bahasa Jawa SMP di Kota Semarang
Authors: Ardiyana Vindi Putri 
Published: November 2020

ABSTRAK
Penelitian ini bertujuan untuk (1) mendeskripsikan karakteristik kebutuhan guru dan siswa terhadap buku cerita bergambar berbasis Serat Wulangreh Pupuh Pangkur untuk pembelajaran bahasa Jawa di SMP Kota Semarang, (2) membuat prototipe buku cerita bergambar berbasis Serat Wulangreh Pupuh Pangkur, dan (3) mendeskripsikan hasil validasi ahli mengenai prototipe buku cerita bergambar berbasis Serat Wulangreh Pupuh Pangkur. Penelitian ini menggunakan metode penelitian pengembangan (Research and Development) dengan tahapan yaitu (1) potensi dan masalah, (2) pengumpulan data, (3) desain produk, (4) validasi desain, dan (5) revisi desain. Data pada penelitian ini adalah amanat dari Serat Wulangreh Pupuh Pangkur dan kebutuhan terhadap buku cerita bergambar berbasis Serat Wulangreh Pupuh Pangkur. Sumber data dari penelitian ini adalah Serat Wulangreh Pupuh Pangkur, guru dan siswa. Teknik pengumpulan data menggunakan wawancara dan penyebaran angket. Analisis data pada penelitian ini adalah deskriptif kualitatif. Penelitian ini menghasilkan buku cerita bergambar berjudul Sinau Wulangreh Pupuh Pangkur. Setelah buku tersebut disusun, selanjutnya dilakukan uji validasi oleh ahli materi dan ahli media untuk mendapatkan saran dan perbaikan buku. Saran dan perbaikan dari ahli mencakup aspek materi, penyajian dan kegrafikaan.
Kata Kunci : buku cerita bergambar, Serat Wulangreh Pupuh Pangkur, pengembangan.

ABSTRACK
The aims of this research are to (1) describe the characteristics of teachers and students’ need to a picture book with Serat Wulangreh Pupuh Pangkur base for Javanese Language learning process in SMP Kota Semarang, (2) to make a picture book prototype with Serat Wulangreh Pupuh base, and (3) describe the expert validation outcome about a picture book prototype with Serat Wulangreh Pupuh Pangkur base. This research uses research and development. The research stages were potential and problems, data collection, product design, design validation and design revisions. The data in this study are mandated by Serat Wulangreh Pupuh Pangkur and the need for a picture book based on Serat Wulangreh Pupuh Pangkur. Sources of data from this study are Serat Wulangreh Pupuh Pangkur, teachers and students. The technique of collecting data uses interviews and questionnaires. Data analysis in this study is descriptive qualitative. This research produces a picture book entitled Sinau Wulangreh Pupuh Pangkur as teachers and students’ need. After this book is created, there would be a validity test by the material and media experts to have suggestion and revision. The scope of suggestion and revision by the experts are material, serving, and graphics.
Keywords: a picture book, Serat Wulangreh Pupuh Pangkur, development.
Pengembangan Media Pansus Raja (Papan Susun Aksara Jawa) unttuk Pembelajaran Aksara Jawa Siswa Kelas III Sekolah Dasar di Kabupaten Semaranng
Authors: Setiyani Angela Wyda , Sukoyo Joko , Hardyanto Hardyanto 
Published: November 2020

Students of Grade III of Elementary school began to be taught Javanese script. The student has some problems when studying Javanese script that is difficult to distinguish and the use of media of learning of Javanese script is limited. Based on these problems found the idea of developing the media of Pansus Raja for the learning of third-grade students of Elementary School in Semarang regency. The problems in this research are: (1) how the needs of teachers and students of the third grade of Elementary school in Semarang regency to Pansus Raja, (2) how to develop the media of Pansus Raja, and (3) how to validate expert lecturers. The method used in this research is Research and Development (RnD) using a data analysis technique that is descriptive qualitative technique. After Pansus Raja was created, then tested to the expert lecturers. The revitalized Pansus Raja tried out to Elementary School. The result of the trial is the media Pansus Raja proved to increase students' understanding of Javanese script.
Pluralisme Sosial Keagamaan Menuju Karakter Bangsa Yang Shalih
Authors: Hamdi Zahratunnisa 
Published: November 2020

The questioning argued that the social problems of the community were directed at the 2005 MUI fatwa which prohibits pluralism. MUI is seen as wrongly assessing the addition of the word "ism" as an ideological signification of the root word "plural", considering that the word pluralism in various linguistic and encyclopaedia literatures has more than just a theological meaning, but also a social meaning. However, critics have never provided a solution in the form of a substitute for the MUI version of pluralism. Besides, some of them are trapped in claiming to support the doctrine of equality of truth for all religions which is banned by MUI even though they try to appear objective at first. The method used in this research is qualitative research methods with a normative approach. The results of the research stated that MUI strives to fight for the harmony of Indonesia's pluralistic society through its formulated “plurality” inclusivism in the hope of realizing peace and harmony in a multi-religious nation. This paper also seeks several sides of the pluralism perspective in philosophical, religious and historical literature, and the possibility of applying its socio-political meaning in realizing the moral and righteous character of a national society.Keywords: Pluralism, Theology, Social Religion, Diversity AbstrakPenyoaalan berdalih persoalan sosial masyarakat diarahkan kepada fatwa MUI tahun 2005 yang mengharamkan faham pluralisme. MUI dipandang salah menilai tambahan kata “isme” sebagai penyifatan ideologis terhadap kata dasar “plural”, mengingat kata pluralisme dalam berbagai literatur kebahasaan dan ensiklopedi memiliki arti lebih dari sekedar makna teologis, tetapi juga makna sosial. Akan tetapi, para pengkritik tidak kunjung memberikan solusi berupa kata pengganti bagi pluralisme versi MUI. Di samping, diantara merekapun terjebak untuk menyatakan mendukung doktrin persamaan kebenaran semua agama yang diharamkan MUI meskipun berusaha terlihat objektif di awal. Metode yang digunakan dalam penelitian ini adalah Metode Penelitian Kualitatif dengan pendekatan normatif. Hasil penelitian menyatakan bahwan MUI berupaya memperjuangkan harmoni masyarakat majemuk Indonesia melalui inklusivisme “pluralitas” yang dirumuskannya dengan harapan dapat mewujudkan kedamaian dan kerukunan bangsa yang multi agama. Tulisan ini pun mengupayakan beberapa sisi pandang pluralisme dalam literatur filsafat, agama dan kesejarahan, dan kemungkinan penerapan makna sosial politisnya dalam mewujudkan karakter masyarakat berbangsa yang bermoral dan shalih.Kata kunci: Pluralisme, Teologis, Sosial Agama, Keberagaman
FOOD PROCESSING AND FERMENTATION STUDIES ON REDUCTION OF PHYTIC ACID IN TRITICUM AESTIVUM AND SORGHUM BICOLOR (L.)
Authors: VENKATASUBBAIAH RASHMI , Konasur Sanjay Rajesh 
Published: November 2020

Wheat and sorghum are the main dieter cereals for millions of people in Africa and Asia. Their nutritive value in wheat and sorghum is limited due to presence of several anti-nutritional substances such as tannins, phytic acid, proteinase inhibitors and cyanogenic glycosides. Phytic acid (PA) or myo-inositol hexakisphosphate (IP6) in salt form known as phytate is the principal storage form of phosphorus in plant tissues, typically accounting for 60-90% of total seed phosphorous. Phytase enzyme catalyses the hydrolysis of phytate and releases the phosphate for absorption. This study explains the use of different methods for the reduction of PA content in wheat and sorghum such as soaking, cooking, roasting and fermentation using lactic acid bacteria (LAB). Among all the methods, fermentation using LAB was found to be effective in reduction of PA. LAB was isolated from the curd sample and fermentation of wheat and sorghum flour was carried out to reduce the PA content. The effect of different fermentation conditions such as pH, temperature and incubation time was also carried out. The results have shown that the optimal fermentation conditions for reduction of PA in wheat and sorghum flour using LAB were; pH- 6 (wheat) and 6.5 (sorghum), temperature 32 o C (wheat) and 37 o C (sorghum) and fermentation time of 4 to 6 h for both wheat and sorghum. The percentage of reduction of PA using fermentation process was compared with the other methods such as soaking, cooking and roasting process.
Study on genetic variations of the D-loop region in three Vietnamese ethnic groups Kinh, Lolo and Lahu
Authors: Duong Nguyen Thuy , Van Phong Nguyen , Thy Ngoc Nguyen , Van Hai Nong 
Published: November 2020

Mitochondrial DNA (mtDNA) sequence analysis has been widely used to investigate population genetic and evolutional history of different ethnic groups worldwide. In this study, the D-loop region of 119 Vietnamese individuals belonging to three different ethnic groups was sequenced and compared with reference mtDNA on rCRS for genetic variations. Total 218 genetic polymorphisms were found in this population, among which 48 variations appeared with frequencies of more than 0.1. Further statistical analysis showed that there were 23, 13 and 24 single nucleotide polymorphisms (SNP) distributed differently between Kinh vs. Lolo, Kinh vs. Lahu , and Lolo vs. Lahu groups, respectively. The mean pairwise genetic distances between each pair of ethnic groups were 0.0101, 0.0098 and 0.0092 for Kinh - Lolo, Kinh - Lahu, Lolo - Lahu, respectively. This indicated that although the Lolo and Lahu ethnic groups both belong to the Tibeto-Burman language group, the genetic distance between them was no closer than the genetic distances between them and the Kinh group of the Viet-Muong (Austroasiatic) language group. This study was the first research on the D-loop region of the Lolo and Lahu populations in Vietnam. These results provided more data for exploring the genetic background and the history of those ethnic groups as well as other minority ethnic groups in Vietnam.
Terme ou formule ? Réflexions autour de l’utilisation du mot migrant dans le discours spécialisé et médiatique
Authors: Kovács Máté 
Published: November 2020

Cet article se propose de porter un regard critique sur l’utilisation du mot migrant dans le discours spécialisé et médiatique. Depuis la crise migratoire de 2015, le mot migrant ne cesse d’être employé et recontextualisé dans divers types de discours. Son usage suscite des analyses suivant deux approches : une approche terminologique dans le cas du discours spécialisé, et une approche orientée vers l’analyse du discours (avec la notion de « formule ») dans le cas du discours médiatique. Ces points de vue croisés permettent de saisir les finesses de la notion de migrant telle qu’elle est employée dans le discours pour décrire la réalité contemporaine.
Florestan Fernandes
Authors: Silva Genilson Ferreira da , Santos Wilson da Silva 
Published: November 2020

O presente texto avalia a atuação de Florestan Fernandes entre os anos de 1987 a 1994, na condição de Deputado Federal, quando reafirma sua defesa da educação pública, além de denunciar o racismo persistente em nossas relações sociais. Trata, portanto, da intervenção política de Florestan Fernandes em favor da educação pública e contra o racismo estrutural. Para tanto, este texto analisa os seus discursos na Assembleia Nacional Constituinte, registrados no Diário da Assembleia em 1997, além de avaliar sua proposta de Emenda Constitucional, apresentada em 1994, que recomenda reparação racial por meio da inserção do negro na educação pública e estatal.
Sanitary Hygiene and Formaldehyde Content in Chicken Intestine in Traditional Markets of Jember Regency
Authors: Moelyaningrum Anita Dewi 
Published: November 2020

Latar Belakang: Bahan berbahaya masih sering ditemui pada berbagai macam makanan jajanan yang beredar di masyarakat. Usus ayam dapat diolah menjadi berbagai jenis masakan dan jajanan, seperti sate usus dan keripik usus. Tujuan: Tujuan penelitian ini adalah untuk menganalisa penerapan higine sanitasi proses produksi usus ayam dan identifikasi kandungan formalin. Metode: Metode penelitian ini adalah deskriptif observasional dengan pendekatan cross sectional. Wawancara dan observasi dilakukan pada responden untuk memperoleh data. Populasi dalam penelitian ini adalah 2 pemasok besar (X dan Y) yang memasok usus ayam ke seluruh pasar di kabupaten Jember. Responden dalam penelitian ini adalah pemilik pemasok usus untuk menggali pengetahuan terhadap penggunaan bahan berbahaya pada makanan, serta para pekerja diproses produksi untuk melihat penerapan prinsip hygiene sanitasi. Pengambilan sampel dilakukan dengan Teknik Proportional random. Hasil: Hasil penelitian menunjukkan tingkat pengetahuan 2 pemasok terhadap bahan tambahan pangan berbahaya adalah cukup, hygiene sanitasi pada saat proses produksi meliputi aspek pengolahan, penyimpanan dan pendistribusian adalah cukup. Pengetahuan personal hygiene pekerja adalah cukup sedangkan perilaku implementasi Higiene sanitasi termasuk kategori kurang. Fasilitas sanitasi rumah produksi pada ke 2 pemasok usus ayam masih termasuk kategori kurang. Kesimpulan: Berdasarkan hasil penelitian diketahui bahwa dari 23 sampel usus ayam yang dijual di pasar trsdisional, terdapat satu sampel (4,35%) positif mengandung formalin (kode sampel PT), yang dipasok oleh pemasok X. Formalin kemungkinan ditambahkan oleh oleh pedagang.
Prevention Efforts With Compliance To The Prevention Of Transmission Of Covid-19 To Covid Volunteers
Authors: Alimansur Moh , Quyumi Elfi 
Published: November 2020

Latar Belakang: Penyakit Covid-19 merupakan penyakit yang mudah menular, sehingga dengan cepat bisa menjangkiti banyak orang. Memperlambat penyebaran virus corona (COVID-19) adalah jalan keluar mengakhiri pandemi. Masyarakat dan pihak non-pemerintah dapat berpartisipasi dalam berbagai bentuk kerelawanan dalam penanggulangan bencana dan pengurangan risiko. Tujuan dalam penelitian ini adalah untuk mengetahui hubungan pengetahuan tentang upaya pencegahan penularan COVID-19 terhadap kepatuhan relawan covid dalam upaya pencegahan penularan COVID19. Metode penelitian ini merupakan penelitian observasional dengan pendekatan cross sectional, besar sampel 105 orang relawan covid di Kota Kediri, dengan metode simple random sampling dengan analisa data menggunakan analisis Chi Square test menggunakan software SPSS 19. Hasil: Hasil penelitian menunjukkan sebagian besar relawan covid memiliki pengetahuan yang cukup tentang upaya pencegahan penularan COVID-19. Hampir seluruhnya dari relawan covid tidak patuh dalam menjalankan upaya pencegahan penularan COVID-19. Hasil uji Chi-square test menunjukkan adanya hubungan pengetahuan upaya pencegahan dengan kepatuhan dalam pencegahan COVID-19 pada relawan covid yang ditunjukkan dengan nilai p-value = 0,00 < α = 0,05. Kesimpulan: Pengetahuan yang kurang tentang upaya pencegahan penularan COVID-19 akan berdampak pula pada penurunan kepatuhan relawan covid dalam mencegah penularan COVID-19. Perlu adanya edukasi, aturan dan penyediaan alat pelindung diri bagi relawan covid dalam pencegahan penularan COVID-19.