bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transcriptomic analysis on the effects of melatonin in gastrointestinal carcinomas
Authors: Ao Lu , Li Li , Sun Huaqin , Chen Huxing , Li Yawei , Huang Haiyan , Wang Xianlong , Guo Zheng , Zhou Ruixiang 
Published: October 2019

Abstract
Background : Melatonin has been shown with anticancer property and therapeutic potential for tumors. However, there lacks a systematic study on the molecular pathways of melatonin and its antitumor effects in gastrointestinal carcinomas. Methods : Using the gene expression profiles of four cancer cell lines from three types of gastrointestinal carcinomas before and after melatonin treatment, including gastric carcinoma (GC), colorectal carcinoma (CRC) and hepatocellular carcinoma (HCC), differentially expressed genes (DEGs) and biological pathways influenced by melatonin were identified. Results : There were 17 pathways commonly altered by melatonin in the three cancer types, including FoxO signaling pathways enriched by the upregulated DEGs and cell cycle signaling pathways enriched by the downregulated DEGs, confirmed the dual role of melatonin to tumor growth, pro-apoptosis and anti-proliferation. DEGs upregulated in the three types of cancer tissues but reversely downregulated by melatonin were commonly enriched in RNA transport, spliceosome and cell cycle signaling pathways, which indicate that melatonin might exert antitumor effects through these pathways. Our results further showed that melatonin can downregulate the expression levels of 5-FU resistance-related genes, such as thymidylate synthase in GC and ATR , CHEK1 , BAX and MYC in CRC, suggesting that melatonin might increase the sensitivity of 5-FU in GC and CRC patients. Conclusions : Melatonin exerts the effects of pro-apoptosis and anti-proliferation on gastrointestinal carcinomas, and might increase the sensitivity of 5-FU in GC and CRC patients.
Fatty acid biosynthesis and transcriptional regulation of Stearoyl-CoA Desaturase 1 (SCD1) in Buffalo milk
Authors: Li Zhipeng , Lu Suyu , Cui Kuiqing , Shafique Laiba , Rehman Saif ur , Luo Chan , Wang Zhiqiang , Liu Qingyou 
Published: October 2019

Abstract
Background: Nutritionally, buffalo milk is of immense importance due to high contents of fatty acid (FA), highly energetic and nutritive value. The composition of FA has been regarded as functional food component, which facilitate to manufacture a variety of processed milk products. Moreover, Buffalo milk contains more unsaturated FAs (UFA) such as oleic and linolenic acids, which have been shown to exert a number of physiological effects. However, inadequate information reported about the chemical composition and mechanism of FA synthesis in buffalo milk. Our conducted research hypothesize that, the expression of SCD1 gene could alter the biosynthesis of fatty acid (FA) in epithelial cells of mammary gland and subsequently affect the FA contents in buffalo milk. Present study investigated transcriptional and biological role of Stearoyl-CoA Desaturase 1 (SCD1) in the buffalo mammary epithelial cells (BMECs) during FA and triacylglycerol (TAG) synthesis. Results: Results showed that unsaturated fatty acid contents are much higher in concentration in buffalo milk as compared to Holstein cow. Significantly, higher expression level of FAS, ACACA, SREBP1, PPARG, GPAT and AGPAT genes were also been observed with the expression of the SCD1 in buffalo milk. Moreover, studies showed that, SCD1 gene alteration in BMECs also causes the change in the expression of FA synthesis related genes mentioned above and subsequently affect the FA composition in the cells. Overexpression of SCD1 significantly increased the expression of genes associated with FA and TAG synthesis and also improved the FA and unsaturated FA contents in BMECs, while down-regulation of SCD1 appear opposite consequences. Conclusion: Thus, present study clearly demonstrated that, alteration in the expression of SCD1 plays an important role in FA and TAG synthesis and can directly or indirectly affect biosynthesis and metabolic pathways in BMECs and may be used as a symbol for important mechanism that contributes the characterization of buffalo milk.
Differential basal expression of immune genes confers Crassostrea gigas resistance to Pacific Oyster Mortality Syndrome
Authors: Julien de Lorgeril , Petton Bruno , Lucasson Aude , Perez Valérie , Stenger Pierre-Louis , Dégremont Lionel , Montagnani Caroline , Escoubas Jean-Michel , Haffner Philippe , Allienne Jean-François , Leroy Marc , Lagarde Franck , Vidal-Dupiol Jérémie , Gueguen Yannick , Mitta Guillaume 
Published: October 2019

Abstract
Background As a major threat to the oyster industry, Pacific Oyster Mortality Syndrome (POMS) is a polymicrobial disease affecting the main oyster species farmed across the world. POMS affects oyster juveniles and became panzootic this last decade, but POMS resistance in some oyster genotypes has emerged. While we know some genetic loci associated with resistance, the underlying mechanisms remained uncharacterized. So, we developed a comparative transcriptomic approach using basal gene expression profiles between different oyster biparental families with contrasted phenotypes when confronted to POMS (resistant or susceptible).Results We showed that POMS resistant oysters show differential expression of genes involved in stress responses, protein modifications, maintenance of DNA integrity and repair, and immune and antiviral pathways. We found similarities and clear differences among different molecular pathways in the different resistant families. These results suggest that the resistance process is polygenic and partially varies according to the oyster genotype.Conclusions We found differences in basal expression levels of genes related to TLR-NFκB, JAK-STAT and STING-RLR pathways. These differences could explain the best antiviral response, as well as the robustness of resistant oysters when confronted to POMS. As some of these genes represent valuable candidates for selective breeding, we propose future studies should further examine their function.
Wall Shear Stress Regulates the Proliferation and Migration of Vascular Smooth Muscle Cells Depending on a TGF-β1 Manner
Authors: Jia Lan , Wei Fang , Wang Lihua , Chen Haiyan , Yu Haibo , Lu Zhi , Wang Bo , Jiang Aili 
Published: October 2019

Abstract
Background: Venous intimal hyperplasia (VIH) is the main cause of arteriovenous fistula (AVF) dysfunction. Hemodynamic forces have an important role in VIH. The proliferation and migration of vascular smooth muscle cells (VSMCs) play a crucial role in the development of VIH and TGF-β1 just has the biological function of inducing proliferation and migration of VSMCs. We use parallel plate flow chamber system to simulate different shear stress and investigate whether shear stress regulate VSMCs proliferation and migration through TGF-β1 Methods: Shear stress (SS) was simulated with an ECs/VSMCs cocultured parallel plate flow chamber system. The coculture system was established by plating cells on the two sides of polyethylene terephthalate membrane. The EC side was subjected to different shear stress (Low-SS, Normal-SS and Oscillating-SS), whereas the opposite VSMCs side was maintained under static conditions. Computational fluid dynamics were applied to three-dimensional models of ECs/VSMCs cocultured flow chamber system to estimate the velocity and WSS. The expression of TGF-β1 were analyzed by immunofluorescence assay. VSMCs proliferation and migration assay was performed with the BrdU kit and Transwell system. Results: The expression of TGF-β1 was significantly up-regulated following application of Low-SS and Oscillating-SS, and the distribution of TGF-β1 was transferred to the cell membrane, compared with the static group. The migration and proliferation of cocultured VSMCs were significantly up-regulated after Low-SS and Oscillating-SS. Conclusion: Our results suggest that Low-SS and Oscillating-SS exerts atherosclerotic influences on the ECs and VSMCs in a TGF-β1-dependent process. TGF-β1 increases the proliferation and migration of VSMC and is thought to be a pro-atherogenic effect, which can be used as a new therapeutic target for the treatment of AVF dysfunction. The formation and development of VIH in AVF may be a local hyperplasia process by shear stress-TGF-β1 regulation, which provides new insights into the mechanisms of neointimal hyperplasia.
Identification of Hemolysin encoding Genes and their Association with Antimicrobial Resistance Pattern among Clinical Isolates of Coagulase-Negative Staphylococci
Authors: Nasaj Mona , Saeidi Zahra , Asghari Babak , Roshanaei Ghodratollah , Arabestani Mohammad 
Published: October 2019

Abstract
Objection : Coagulase-negative staphylococci (CoNS) are considered opportunistic pathogens which capable of producing several toxins, enzymes and also resistance genes. The current study aimed to determine the occurrence of different hemolysins and patterns of antibiotic resistance among CoNS species. Results : The highest frequency of antibiotic resistance was observed against cefoxitin in 49 isolates (53.8%), and the lowest resistance was against novobiocin in 5 isolates (5.5%). None of the isolates were resistant to vancomycin. The prevalence of hla, hla_yidD, hld, and hlb genes were determined as 87.9%, 62.6%, 56%, and 47.3%, respectively. The hla/yidD and hld genes were detected in 69.4% of S. epidermidis and the hla gene in 94.6% of S. haemolyticus ; hlb gene was detected in 53.1% of the S. epidermidis isolates. mecA gene was identified in 50 (55%) of the CoNS isolates. In conclusion, the results of statistical analysis showed that the hld gene had a significant association with resistance to levofloxacin and erythromycin and the hlb with clindamycin resistance. The results of this study showed that there is a significant relationship between hemolysin encoding genes and antibiotic resistance patterns; therefore, detection of virulence factors associated with antibiotic resistance has become a significant issue of concern.
Association between multi-component initiatives and physical activity-related behaviors: interim findings from the Healthy Schools Healthy Communities Initiative
Authors: Calise Tamara , Spitzer Natalie , Ruggiero Laura , Ryder Amanda , Wingerter Chloe , Hatcher Ashley 
Published: October 2019

Abstract
Background: Little is known about the intensity of multi-component initiatives (MCI) inclusive of any combination of programs, practices, policies, and environmental changes (P3E) on youth behaviors. Methods: The Healthy Schools Healthy Communities (HSHC) initiative worked to increase access to PA opportunities as part of 33 MCIs targeting school districts and respective communities. The purpose of this study was to assess whether MCIs with higher scoring PA-related strategies were associated with improved PA and sedentary behaviors among youth. PA-related interventions were assigned point values based on three characteristics: 1) purpose of initiative); 2) duration; and 3) reach. An overall composite score of all activities was calculated for each school district. Multivariate longitudinal regressions were applied, controlling for measurement period, Cohort, and district enrollment. Results: P3E scores ranged from 0.3 to 3.0 with 20% considered “higher-scoring” (score > 2.1) and 47% considered “lower-scoring” ( < 1.2 ). Average composite district P3E scores more than tripled over the evaluation period, rising from 14.8 in the first grant year to 32.1 in year 2, 41.1 in year 3, and 48.1 in year 4. For each additional point increase in average composite district P3E intensity score, the number of days per week that students reported being physically active for at least 60 minutes increased by 0.010 (p<0.01). For each additional point increase in district P3E intensity score, the number of hours per weekday that students reported engaging in screen time activities decreased by 0.006 hours (p<0.5). Conclusions: MCIs are difficult to evaluate given the variations within which they are implemented. Findings from this study suggest the value of a systematic scoring approach in assessing MCIs. In addition to tracking intensity over time, scoring can provide justification for, or against, an individual activity and ways to increase the likelihood of the MCI impacting population-health outcomes.
Growth Response of Greenhouse-Produced Muskmelon and Tomato to Sub-Surface Drip Irrigation and Soil Aeration Management Factors
Authors: Li Yuan , Niu Wenquan , Cao Xiaoshu , Zhang Mingzhi , Wang Jingwei , Zhang Zhenxing 
Published: October 2019

Abstract
Background: Hypoxia causes injury and yield loss. Soil aeration has been reported to accelerate the growth of plants and increase crop yield. The aim of this study was to examine growth response of greenhouse-produced muskmelon to 3 levels of sub-surface drip irrigation (I), 3 different installation depths of drip laterals in the soil (D), and 4 levels of supplemental soil aeration frequency (A). A fractional factorial experiment was designed to examine these treatment effects on marketable fresh fruit yield, leaf area index during 3 growth stages, and dry matter partitioning at harvest. In addition, we studied the response of fruit yield and dry matter of tomato to 2 levels of burial depths of subsurface tubing in combination with 3 frequency levels of soil aeration. Results: Results showed that soil aeration can positively influence the yield, leaf area index, dry matter and irrigation use efficiency of the muskmelon (p<0.05). The fruit yield of muskmelon and tomato were increased by 21.5% and 30.8% respectively with 1-d and 2-d aeration intervals compared with the no aeration treatment. Conclusions: The results suggest that soil aeration can positively impact the plant root zone environment and more benefits can be obtained with aeration for both muskmelon and tomato plants.
Patient Complications After Interscalene Block: A Retrospective Comparison of Liposomal Bupivacaine to Non-Liposomal Bupivacaine.
Authors: hutchins jacob , Habeck Jason , Novaczyk Zac , Campbell Richard , Creedon Christopher , Spartz Ellen , Richter Michael , Wolter Jeremy , Suryawanshi Gaurav , Kaizer Alexander , Berg Aaron A. 
Published: October 2019

Abstract
Background The purpose of this study was to investigate if the addition of liposome bupivacaine (LB) to an interscalene block (ISB) had an effect on the number of patients with surgical or block related complications.Methods This was a single-center retrospective chart view was performed identifying patients who received an ISB from January 1, 2014 through April 26, 2018 at the University of Minnestoa. 1,518 patients were identified who received an ISB (LB =784, non-liposomal bupivacaine =734). Patients were divided into two groups those who did receive liposome bupivacaine in their ISB and those who did not receive liposome bupivacaine in their ISB. Medical records were individually reviewed for surgical procedure, block medications, complications related to the block or surgical procedure, phone calls to the healthcare system for issues related to opioids or pain within 3 and within 30 days, readmissions within 30 days, and emergency room visits for complications within 3 and 30 days.Results There was no significant difference in the number of patients with surgical or anesthetic complications. Only phone calls for pain within 3 days was significantly different. The LB group had 3.2% of patients call compared to 5.6% in the non-liposomal bupivacaine group (aOR=1.71 [95% CI: 1.04-2.87], p=0.036). We found no significant difference in any of the other secondary outcomes.Conclusions The use of LB in an ISB demonstrated no significant difference compared to non-liposomal bupivacaine in numbers of complications, emergency room visits, and readmissions.
Anesthetics and long-term survival after cancer surgery—total intravenous versus volatile anesthesia: a retrospective study
Authors: Hong Boohwi , Lee Sunyeul , Kim Yeojung , Lee Minhee , Youn Ann Misun , Rhim Hyun , Hong Seok-Hwan , Kim Yoon-Hee , Yoon Seok-Hwa , Lim Chaeseong 
Published: October 2019

Abstract
Background Intravenous anesthesia has been reported to have a favorable effect on the prognosis of cancer patients. This study was performed to analyze data regarding the relation between anesthetics and the prognosis of cancer patients in our hospital. Methods The medical records of patients who underwent surgical resection for gastric, lung, liver, colon, and breast cancer between January 2006 and December 2009 were reviewed. Depending on the type of anesthetic, it was divided into total intravenous anesthesia (TIVA) or volatile inhaled anesthesia (VIA) group. The 5-year overall survival outcomes were analyzed by log-rank test. Cox proportional hazards modeling was used for sensitivity. Results The number of patients finally included in the comparison after propensity matching came to 729 in each group. The number of surviving patients at 5 years came to 660 (90.5%) in the TIVA and 673 (92.3%) in the VIA. The type of anesthetic did not affect the 5-year survival rate according to the log-rank test ( P = 0.21). Variables associated with a significant increase in the hazard of death after multivariable analysis were male sex and metastasis at surgery. Conclusion There were no differences in 5-year overall survival between two groups in the cancer surgery.
Acupuncture for patients with insomnia disorder using resting-state functional magnetic resonance imaging: a protocol for a Randomised Controlled Trial
Authors: guo jing , Yu Siyi , Liu Chunhong , Wang Guiling , li Bin 
Published: October 2019

Abstract
Background: Insomnia is among the most prevalent sleep-related disorders. Insomnia disorder is associated with a brain hyperarousal state manifested by abnormal regional brain activity and resting-state functional connectivity (rs-FC). Acupuncture improves sleep quality and modulates the hyperarousal state; however, the underlying neurobiological basis for improved sleep quality is poorly understood. The purpose of this clinical trial is to study the efficacy of acupuncture in the treatment of insomnia disorder. In addition, the neural mechanism by which acupuncture affects insomnia disorder will be explored utilizing resting-state functional magnetic resonance imaging (rs-fMRI) and neuropsychological parameters.
Methods and Design: A randomized, patient-assessor-blinded trial will be conducted. We will randomize (1:1 ratio) 60 eligible patients with insomnia disorder into a real acupuncture group or a sham acupuncture group. Interventions will be administered three times per week over a 4 week period, with an 8 week follow-up period. The healthy control group will consist of 30 age- and sex-matched healthy good sleepers without any treatment intervention.
All participants will undergo neuropsychological and rs-fMRI evaluations. The change of Pittsburgh Sleep Quality Index (PSQI) scores is the primary outcome parameter. The secondary outcome parameters include the Hyperarousal scale (HAS), rs-fMRI measurements, the Fatigue scale-14 (FS-14), the Hamilton depression scale (HAMD), the Hamilton anxiety scale (HAMA), a sleep diary, and an actigraph.
Assessment of all parameters will be performed at baseline, post-treatment, and during follow-up. Analyses will be implemented based on intention-to-treat.
Discussion: The study results will be utilized to clarify the effectiveness and elucidate the mechanism by which acupuncture improves sleep quality in patients with insomnia disorder.
Comparison of 22G Standard and Franseen Needles in Endoscopic Ultrasound-Guided Fine-Needle Aspiration for Diagnosing Pancreatic Mass Lesions: Study Protocol for a Randomized Controlled Trial
Authors: Itonaga Masahiro , Yasukawa Satoru , Shimokawa Toshio , Takenaka Mamoru , Fukutake Nobuyasu , Ogura Takeshi , Sakagami Junichi , Shiomi Hideyuki , Okura Yasushi , Inatomi Osamu , Matsumoto Hisakazu , Kurita Akira , Suzuki Azumi , Tanaka Kiyohito , Kitano Masayuki 
Published: October 2019

Abstract
Background Endoscopic ultrasound-guided fine-needle aspiration (EUS-FNA) was developed with the aim of further improving the diagnostic performance of endoscopic ultrasound. Although novel puncture needles have been specifically designed for collecting sufficient tissue specimens, clinical studies have indicated no clear difference in diagnostic performance between these novel needles and conventional puncture needles. Recently, a needle with Franseen geometry was developed specifically for EUS-FNA biopsy. Due to the characteristic shape of its tip, the Franseen needle is expected to be effective for scraping tissues, thus potentially increasing the diagnostic accuracy of EUS-FNA biopsy. We plan to carry out a prospective, multicenter, open-labeled, randomized, controlled trial to compare conventional and Franseen needles in terms of the diagnostic accuracy of EUS-FNA for evaluating the malignancy of pancreatic mass lesions. Methods/design The study will enroll 520 patients with pancreatic mass managed at any of 21 participating endoscopic centers. Lesion samples obtained using 22G conventional and Franseen needles will be assessed to compare the efficacy and safety of these two types of needles in EUS-FNA for evaluating the malignancy of mass lesions in the pancreas. Tissue samples will be fixed in formalin and processed for histologic evaluation. For the purpose of this study, only samples obtained with the first needle pass will be used for comparing the: (i) accuracy of the malignancy diagnosis, (ii) sensitivity and specificity for the malignancy diagnosis, (iii) procedure completion rate, (iv) sample cellularity, and (v) incidence of complications. Discussion The outcomes of this study may provide insight into the optimal needle choice for evaluating the malignancy of pancreatic solid lesions, thus aiding in the development of practice guidelines for pancreatic diseases.