bluuebunny/crossref_metadata_2025_split huggingface dataset:
EO771, is it a well‐characterized cell line for mouse mammary cancer model? Limit and uncertainty
Authors: Le Naour Augustin , Rossary Adrien , Vasson Marie‐Paule 
Published: October 2020

AbstractAmong mouse mammary tumor models, syngeneic cell lines present an advantage for the study of immune response. However, few of these models are well characterized. The tumor line EO771 is derived from spontaneous breast cancer of C57BL/6 mice. These cells are widely used but are referenced under different names: EO771, EO 771, and E0771. The characteristics of the EO771 cells are well described but some data are contradictory. This cell line presents the great interest of developing an immunocompetent neoplastic model using an orthotopic implantation reflecting the mammary tumors encountered in breast cancer patients. This review presents the phenotype characteristics of EO771 and its sensitivity to nutrients and different therapies such as radiotherapy, chemotherapy, hormone therapy, and immunotherapy.
Fluorescence Response and Self‐Assembly of a Tweezer‐Type Synthetic Receptor Triggered by Complexation with Heme and Its Catabolites
Authors: Hisamatsu Yosuke , Otani Koki , Takase Hiroshi , Umezawa Naoki , Higuchi Tsunehiko 
Published: October 2020

AbstractThere is increasing interest in the development and applications of synthetic receptors that recognize target biomolecules in aqueous media. We have developed a new tweezer‐type synthetic receptor that gives a significant fluorescence response upon complexation with heme in aqueous solution at pH 7.4. The synthetic receptor consists of a tweezer‐type heme recognition site and sulfo‐Cy5 as a hydrophilic fluorophore. The receptor–heme complex exhibits a supramolecular amphiphilic character that facilitates the formation of self‐assembled aggregates, and both the tweezer moiety and the sulfo‐Cy5 moiety are important for this property. The synthetic receptor also exhibits significant fluorescence responses to biliverdin and bilirubin, but shows very weak fluorescence responses to flavin mononucleotide, folic acid, and nicotinamide adenine dinucleotide, which contain smaller π‐scaffolds.
Liposome‐Boosted Peroxidase‐Mimicking Nanozymes Breaking the pH Limit
Authors: Chen Qiaoshu , Liu Yibo , Liu Jianbo , Liu Juewen 
Published: October 2020

AbstractPeroxidase‐mimicking nanozymes such as Fe3O4 nanoparticles are promising substitutes for natural enzymes like horseradish peroxidase. However, most such nanozymes work efficiently only in acidic conditions. In this work, the influence of various liposomes on nanozyme activity was studied. By introducing negatively charged liposomes, peroxidase‐mimicking nanozymes achieved oxidation of 3,3′,5,5′‐tetramethylbenzidine (TMB) in neutral and even alkaline conditions, although the activity towards anionic 2,2′‐azino‐bis(3‐ethylbenzothiazoline‐6‐sulfonic acid) (ABTS) was inhibited. The Fe3O4 nanoparticles adsorbed on the liposomes without disrupting membrane integrity as confirmed by fluorescence quenching, dye leakage assays, and cryo‐electron microscopy. Stabilization of the blue‐colored oxidized products of TMB by electrostatic interactions was believed to be the reason for the enhanced activity. This work has introduced lipids to nanozyme research, and it also has practically important applications for using nanozymes at neutral pH, such as the detection of hydrogen peroxide and glucose.
Enhanced coping and self‐efficacy in caregivers of stem cell transplant recipients: Identifying mechanisms of a multimodal psychosocial intervention
Authors: Jacobs Jamie M. , Nelson Ashley M. , Traeger Lara , Waldman Lauren , Nicholson Showly , Jagielo Annemarie D. , D'Alotto Jennifer , Greer Joseph A. , Temel Jennifer S. , El‐Jawahri Areej 
Published: October 2020

BackgroundIn a recent trial, a 6‐session intervention (BMT‐CARE) integrating medical information with cognitive‐behavioral strategies improved quality of life (QOL), mood, coping skills, and self‐efficacy for family/friend caregivers of hematopoietic stem cell transplantation (HCT) recipients. This study examined whether improvements in coping and self‐efficacy mediated the intervention effects on QOL and mood.MethodsFrom December 2017 to April 2019, 100 caregivers of HCT recipients were enrolled into a randomized clinical trial of BMT‐CARE versus usual care. Caregivers completed self‐report measures of QOL (CareGiver Oncology Quality of Life questionnaire), depression and anxiety symptoms (Hospital Anxiety and Depression Scale), coping skills (Measure of Current Status), and self‐efficacy (Cancer Self‐Efficacy Scale–Transplant) at enrollment (before HCT) and 60 days after HCT. Causal mediation regression models were used to examine whether changes in coping and self‐efficacy mediated intervention effects on QOL as well as depression and anxiety symptoms.ResultsImprovements in 60‐day QOL in patients assigned to BMT‐CARE were partially mediated by improved coping and self‐efficacy (indirect effect, 6.93; SE, 1.85; 95% CI, 3.71‐11.05). Similarly, reductions in 60‐day depression and anxiety symptoms were partially mediated by improved coping and self‐efficacy (indirect effect for depression, −1.19; SE, 0.42; 95% CI, −2.23 to −0.53; indirect effect for anxiety, −1.46; SE, 0.55; 95% CI, −2.52 to −0.43). Combined improvements in coping and self‐efficacy accounted for 67%, 80%, and 39% of the total intervention effects on QOL and depression and anxiety symptoms, respectively.ConclusionsCoping and self‐efficacy are essential components of a brief psychosocial intervention that improves QOL and mood for caregivers of HCT recipients during the acute recovery period.LAY SUMMARY

A 6‐session program (BMT‐CARE) focused on providing medical information, caregiving skills, and self‐care and coping strategies has been previously reported to improve the quality of life and mood of caregivers of hematopoietic stem cell transplantation recipients in comparison with caregivers who receive care as usual.

Using statistical models, this study suggests that learning coping skills and improving self‐efficacy are the most essential components of this program that likely lead to better quality of life and mood for caregivers.
Localization and hypersecretion of nerve growth factor in breast phyllodes tumors: Evidence from a preliminary study
Authors: Kumar Ashutosh , Raza Khursheed , Nag Tapas C. , Srivastava Anurag , Sehgal Ritu 
Published: October 2020

AbstractBackgroundThe pathophysiology of the breast phyllodes tumors is uncertain. Currently, wide surgical removal is the only available treatment option. The histopathological diagnosis of phyllodes tumors is often confused with that of fibroadenomas due to a striking histological resemblance.AimTo identify a distinctive biomarker for phyllodes tumors of the breast.Methods and ResultsFresh human breast tissue was obtained from surgically excised breast phyllodes and fibroadenoma tumors (test), breast cancer (positive control) and normal breast tissue (negative control). Immunohistochemistry and Sandwich ELISA were performed for the detection of nerve growth factor (NGF) in test and control tissues. A marked difference in NGF expression was detected in phyllodes tumors compared to fibroadenomas. The maximum NGF expression was observed in phyllodes tissue followed by cancer tissue, and the least expression in fibroadenomas (3‐5 times less than in phyllodes; comparable with normal breast tissue).ConclusionNGF secretion by a benign breast tumor is not known in literature. This study reports abundant NGF secretion by breast phyllodes, raising the possibility of its potential role in tumor pathogenesis and progression that can be exploited therapeutically. Additionally, NGF may be used as a distinct biomarker of phyllodes tumors, for differentiating them from fibroadenomas during histopathology.
Analysis on ceiling space radio wave propagation in indoor environment
Authors: Sato Yoshihito , Ishiyama Ryo , Takeda Shigeki 
Published: October 2020

AbstractIn this paper, received power levels in 420 M/920 M/2.4G/5.3 GHz bands for sensor networks and WLANs are obtained by ray‐trace analysis in an office environment and the detail properties of propagation paths are analyzed. As a result, 25% average power of the total power arrives through ceiling spaces in the rooms where electromagnetic waves are hard to arrive through the walls. This means that the power remains in −6 dB even though the propagation paths are blocked by several metallic furniture beside the walls. In this indoor environment, the propagation paths through ceiling spaces can be relatively good for wireless communication. The results confirm that modeling of structures in ceiling spaces is necessary to obtain practical received power levels.
What is agentic about the Spatial Agency Bias? How pragmatic relevance contributes to the spatial representations of actions
Authors: Halicki Katharina Theresa , Suitner Caterina , Vogel Tobias , Wänke Michaela 
Published: October 2020

AbstractThe mental imagination of (social) actions has been shown to follow a left‐to‐right trajectory, with the thematic agent associated with the left position (Spatial Agency Bias, Suitner & Maass in Advances in Experimental Social Psychology, 53, 2016, p. 245–301). For example, individuals asked to choose a picture that visualizes the sentence “Tom kicks George” tend to choose an image where the agent, Tom, is positioned on the left‐hand side rather than on the right‐hand side. However, as an alternative to the thematic role of the agent, such findings may reflect a mental representation following pragmatic relevance. Specifically, a pragmatic perspective holds that word order and syntactic functions are strategic devices to communicate that the element is important for the sentence. Thus, positioning in the described picture‐matching task may actually reflect the agent's pragmatic relevance instead of agency per se. As a test, we vary whether sentences are written in the active versus the passive voice. Results from five studies indicate that the passive voice results in the tendency to place the agent on the right‐hand side rather than on the left‐hand side of a picture. Instead, the acted‐upon person is positioned on the left‐hand side of a picture. A sixth experiment reveals that for the passive voice, the agent is still seen as more agentic than the receiver, but is considered less relevant. The findings are congruent with the proposed pragmatic relevance account. Implications for the Spatial Agency Bias as well as for building mental representations in general are discussed.
Effect of Ru Substitution in La0.85Sr0.15CoO3 towards Oxygen Evolution Reaction: Activity of Ionic Ru
Authors: Vats Vishal , Singhal Aditi 
Published: October 2020

AbstractNano crystalline La0.85Sr0.15CoO3 and ruthenium doped compounds (La0.85Sr0.15Co0.9975Ru0.0025O3, La0.85Sr0.15Co0.995Ru0.005O3, La0.85Sr0.15Co0.99Ru0.01O3) are synthesized using solution combustion method. Completely characterized samples are studied for electrochemical OER in neutral and basic medium. Significant enhancement in catalytic activity is noticed once Ru is substituted in the cobalt site. With higher doping level of Ru, capacitance also increases as depicted in the CV behavior. Tafel slope measurements indicate that Ru substitution has profound effect as enhancement in the exchange current density is observed in neutral K2SO4 medium. Enhancement is anticipated due to substitution of Ru, as the RuO2 mixed catalyst does not give similar activity.
Increased yield of enzymatic synthesis by chromatographic selection of different N‐glycoforms of yeast invertase
Authors: Andjelković Uroš , Gudelj Ivan , Klarić Thomas , Hinneburg Hannes , Vinković Marijana , Wittine Karlo , Dovezenski Nebojša , Vikić‐Topić Dražen , Lauc Gordan , Vujčić Zoran , Josić Djuro 
Published: October 2020

AbstractInvertases are glycosidases applied for synthesis of alkyl glycosides that are important and effective surfactants. Stability of invertases in the environment with increased content of organic solvent is crucial for increase of productivity of glycosidases. Their stability is significantly influenced by N‐glycosylation. However, yeast N‐glycosylation pathways may synthesize plethora of N‐glycan structures. A total natural crude mixture of invertase glycoforms (EINV) extracted from Saccharomyces cerevisiae was subfractionated by anion‐exchange chromatography on industrial monolithic supports to obtain different glycoforms (EINV1–EINV3). Separated glycoforms exhibited different stabilities in water‐alcohol solutions that are in direct correlation with the amount of phosphate bound to N‐glycans. Observed differences in stability of different invertase glycoforms were used to improve productivity of methyl β‐d‐fructofuranoside (MF) synthesis. The efficiency and yield of MF synthesis were improved more than 50% when the most stabile glycoform bearing the lowest amount of phosphorylated N‐glycans is selected and utilized. These data underline the importance of analysis of glycan structures attached to glycoproteins, demonstrate different impact of N‐glycans on the surface charge and enzyme stability in regard to particular reaction environment, and provide a platform for improvement of yield of industrial enzymatic synthesis by chromatographic selection of glycoforms on monolithic supports.
NATM – Review and Outlook
Authors: Golser Johann , Keuschnig Markus , Weichenberger Franz Peter 
Published: October 2020

AbstractBased on mining experience and historical conventional construction processes a new type of construction has evolved – the New Austrian Tunnelling Method (NATM). NATM changed the view on the rock mass surrounding the tunnel from the forcing to the load‐carrying component. NATM is a proven methodology for conventional tunnelling applied worldwide. New observation– and measurement techniques as well as digital models (e.g. geological model) will be increasingly integrated within the Building Information Modelling (BIM) process. BIM provides a sound basis of vital information for decision support across different project phases.
Astra‐Tunnelerneuerungsmethode – Erneuerung einer nicht armierten Innenschale in Nachtarbeit bei Gewährleistung des Betriebs aller Fahrstreifen in Spitzenzeiten
Authors: Balbi Gianpiero 
Published: October 2020

AbstractTo secure the availability and usability of the motorway network, the Federal Roads Office (Fedro) developed new solutions for the renovation of road tunnels while keeping them open to traffic and initiated a pilot project. The aim is to replace a non‐reinforced inner shell at night and ensure the availability of all lanes during the day. The solution involves partially removing the existing inner shell, then installing a drainage and waterproofing system, and finally installing a new inner lining of self‐compacting concrete during the night.The success of this pilot project led to the decision to renovate two more tunnels without delay. The strategy is based on optimising the basic variant by reducing the lining thickness using ultra‐high performance fibre reinforced cementitious composite (UHPFRC) instead of conventional concrete or installing thin precast segmental lining in UHPFRC instead of conventional formwork. Fedro's innovative method will make it possible to renovate road tunnels over the long term while maintaining daytime traffic flow without massively impacting their substance.
Passing a major fault zone three times: NATM helps TBM to succeed
Authors: Pilgerstorfer Thomas , Hölzl Harald , Moritz Bernd 
Published: October 2020

AbstractThis paper discusses the design and construction of a fault zone of the OeBB Koralmtunnel lot KAT3. Due to the experience gained during the advance of the parallel‐situated southbound tunnel and the aim to minimize the geotechnical hazard for the subsequent excavation of the northbound TBM bore, an extensive redesign regarding the excavation‐concept and ‐method was performed. Five different alternative solutions have been subject to a risk analysis to optimize the required measures and to minimize the cost‐effective risks and hazards to an acceptable level. The set of measures, which was evaluated and juxtaposed, contained solutions for strengthening the rock mass with grouting from the TBM and from aside the bore, conventional tunnelling and backfill with a top heading or full‐face excavation. Finally, the fault zone has been excavated with a conventional top heading drive from a nearby‐situated cross‐passage. Subsequently this tunnel stretch has been excavated by TBM without any construction delay. The paper focuses on highlighting the importance of a consequent application of the observational method and thereof derived measures in the context of a geotechnical safety management plan for controlling the residual risks.
Adjuvant treatment for high‐risk salivary gland malignancies and prognostic stratification based on a 20‐year single institution experience
Authors: Onderdonk Benjamin E. , Vokes Everett E. , Gwede Michael , Blair Elizabeth , Agrawal Nishant , Haraf Daniel J. 
Published: October 2020

AbstractBackground and AimRetrospective analysis of the utility of adjuvant radiation (RT) or chemoradiation (CRT) and identify prognostic features for patients with high‐risk head and neck salivary gland cancers.MethodsFrom 1/1997 to 12/2017, 108 patients underwent surgery, and RT (n = 50) or CRT (n = 58) for positive lymph node(s), extracapsular extension, perineural invasion, lymphovascular space invasion, positive/close margin, and/or grade 3 disease. Outcomes were estimated with the Kaplan‐Meier method. Significant predictors identified through regression analyses were incorporated into multivariable regression (MVA). Toxicities were compared using chi‐square.ResultsThe median follow‐up was 52 months (range: 3‐226). The number of risk factors (RFs) between RT and CRT groups were: 0 to 1 (44% vs 7%), 2 to 3 (48% vs 41%), or 4 to 6 (8% vs 52%), respectively (P < .01). On MVA, stage 3 or 4 disease predicted worse outcomes including overall survival (HR 4.55, P = .01). Increasing number of RFs predicted worse disease‐free survival, distant metastasis‐free survival, and overall survival (2‐3 RFs: HR 3.38, P = .03; 4‐6 RFs: HR 5.78, P < .01), but not locoregional control (P = .54). So, adjuvant CRT may have provided comparable locoregional control for patients with more adverse features, but the CRT did not translate into improved distant control. There was no difference in acute or late grade 3+ toxicities, or parenteral nutrition (P = .98, P = .85, and P = .83), respectively.ConclusionsAdjuvant CRT provides adequate locoregional control in patients with more adverse RFs. The absolute number of RFs serves prognostic significance and should be considered in future prospective trials.
Inhibition of lncRNA DCST1‐AS1 suppresses proliferation, migration and invasion of cervical cancer cells by increasing miR‐874‐3p expression
Authors: Liu Junli , Zhang Jun , Hu Yan , Zou Hongyan , Zhang Xiuzhen , Hu Xiaojun 
Published: October 2020

AbstractBackgroundCervical cancer seriously threatens both the health and life of women. We aimed to investigate whether RNA interference of long non‐coding RNA (lncRNA) DCST1‐AS1 could promote miR‐874‐3p expression to affect the proliferation, migration and invasion of cervical cancer cells.MethodsDCST1‐AS1 expression levels in cervical cancer cells and transfection effects were detected by quantitative reverse transcriptase‐polymerase chain reaction analysis. Proliferation, invasion and migration of cells were separately shown by cell‐counting kit‐8, wound healing and transwell assays, and relative protein expression was determined by western blot analysis. Dual‐luciferase reporter and RNA immunoprecipitation assays verified the interaction of DCST1‐AS1 and miR‐874‐3p.ResultsDCST1‐AS1 expression was increased in cervical cancer tissues and cells. The DCST1‐AS1 expression in Hela and SiHa cells was the highest, and so the cells were selected for the next experiment. Inhibition of DCST1‐AS1 suppressed the proliferation, invasion and migration of cervical cancer cells and decreased the expression of KI67, proliferating cell nuclear antigen, matrix metalloproteinase (MMP)‐2 and MMP‐9. miR‐874‐3p expression was increased when cells were transfected with miR‐874‐3p mimic or shRNA‐DCST1‐AS1‐1, and DCST1‐AS1 expression was down‐regulated when cells were transfected with miR‐874‐3p mimic. DCST1‐AS1 can directly target miR‐874‐3p. Furthermore, inhibition of miR‐874‐3p could effectively alleviate the effect of inhibition of DCST1‐AS1 with respect to the proliferation, invasion and migration of cervical cancer cells.ConclusionsInhibition of DCST1‐AS1 suppressed the proliferation, migration and invasion of cervical cancer cells by increasing miR‐874‐3p expression, which could be alleviated by the inhibition of miR‐874‐3p.
Contact engineered charge plasma junctionless transistor for suppressing tunneling leakage
Authors: Kumar Harendra , Singh Sangeeta , Priyadarshani Kumari Nibha , Ghosh Jayanta , Naugarhiya Alok 
Published: October 2020

AbstractThis work reports contact engineered charge plasma junctionless transistor (CE‐CP‐JLT) to suppress the tunneling leakage current by deploying calibrated exhaustive 2‐D TCAD simulations. This analysis considered the detrimental effect due to the nonohmic source and drain side contacts by including universal Schottky tunneling and band‐to‐band tunneling models. Our study suggests to reduce the source/drain metal contacts to control the carriers tunneling in OFF state. This contact engineering can effectively minimizes the leakage with retained drive current capabilities. This would in turn improve the device switching behavior and ION/IOFF current ratio at least by four orders. The reduction in leakage current leads to decrease in static power consumption. This facilitates the CE‐CP‐JLT deployment in a portable device for longer duration with same power supply. Further, the device sensitivity analysis with respect to electrode contact length, gate oxide thickness, dielectric constant, channel length and silicon film thickness is also analyzed. Moreover, the comparative analysis of device analog behavior is also carried out here.
Low Prevalence of NOTCH2NLC GGC Repeat Expansion in White Patients with Movement Disorders
Authors: Yau Wai Yan , Vandrovcova Jana , Sullivan Roisin , Chen Zhongbo , Zecchinelli Anna , Cilia Roberto , Duga Stefano , Murray Malgorzata , Carmona Susana ,   Genomics England Research Consortium, Chelban Viorica , Ishiura Hiroyuki , Tsuji Shoji , Jaunmuktane Zane , Turner Chris , Wood Nicholas W. , Houlden Henry 
Published: October 2020

AbstractBackgroundThe objective of this study was to determine the prevalence of the GGC‐repeat expansion in NOTCH2NLC in whites presenting with movement disorders.MethodsWe searched for the GGC‐repeat expansion in NOTCH2NLC using repeat‐primed polymerase chain reaction in 203 patients with essential tremor, 825 patients with PD, 194 patients with spinocerebellar ataxia, 207 patients with “possible” or “probable” MSA, and 336 patients with pathologically confirmed MSA. We also screened 30,008 patients enrolled in the 100,000 Genomes Project for the same mutation using ExpansionHunter, followed by repeat‐primed polymerase chain reaction. All possible expansions were confirmed by Southern blotting and/or long‐read sequencing.ResultsWe identified 1 patient who carried the NOTCH2NLC mutation in the essential tremor cohort, and 1 patient presenting with recurrent encephalopathy and postural tremor/parkinsonism in the 100,000 Genomes Project.ConclusionsGGC‐repeat expansion in NOTCH2NLC is rare in whites presenting with movement disorders. In addition, existing whole‐genome sequencing data are useful in case ascertainment. © 2020 The Authors. Movement Disorders published by Wiley Periodicals LLC on behalf of International Parkinson and Movement Disorder Society