bluuebunny/crossref_metadata_2025_split huggingface dataset:
Characterizing the amount and variability of intramuscular fat deposition throughout pork loins using barrows and gilts from two sire lines
Authors: Redifer Jack D , Beever Jon E , Stahl Chad A , Boler Dustin D , Dilger Anna C 
Published: August 2020

Abstract
The objective was to determine the amount and variability of intramuscular fat (IMF) in a pork loin attributable to anatomical chop location, sex, and sire line. Pigs were sired by commercially available terminal Duroc boars selected for meat quality (MQ; n = 96) or lean growth (LG; n = 96) and equally split between barrows and gilts. After slaughter and fabrication, bone-in chops were removed from four locations of each left-side loin (A = 6th rib, B = 10th rib, C = last rib, and D = 4th lumbar vertebrae). An adjacent pair of chops from each location was collected and evaluated for visual color and marbling, subjective firmness, moisture and extractable lipid (IMF) (anterior chop), and Warner–Bratzler shear force (posterior chop). Data were analyzed using the MIXED procedure of SAS as a split-plot design. Homogeneity of variances was tested on raw data using Levene’s test of the GLM procedure and found to be heterogeneous. Thus, a two-variance model was fit using the REPEATED statement of the MIXED procedure, grouped by pig. The mivque(0) option of the VARCOMP procedure was used to calculate the proportion of variability that each factor contributed to the total variance. Barrows (3.64%) produced chops with greater (P &lt; 0.01) IMF content than gilts (3.20%), and barrows (2.14) had greater (P &lt; 0.01) IMF variability than gilts (1.23). Chops from MQ pigs (4.02%) exhibited greater (P &lt; 0.01) IMF content than LG (2.82%), and MQ (1.76) had greater IMF variability (P &lt; 0.01) than LG pigs (0.97). Chops from locations A (3.80%) and D (3.77%) had greater IMF than B (3.34%; P &lt; 0.01), and A, B, and D had greater IMF than C (2.77%; P &lt; 0.01). Variances of IMF also differed (A = 1.44, B = 1.59, C = 1.05, and D = 2.18; P = 0.01) across chop locations. Of the variability in IMF, 33.0% was attributed to sire line, 10.16% to chop location, and 4.01% to sex, with 52.83% not accounted for by these three factors. Location A chops were the most (P &lt; 0.01) tender (2.57 kg) and C chops the least (P &lt; 0.01) tender (2.93 kg), while B and D chops were intermediate and not different from each other. No differences in variability (P = 0.40) of tenderness were observed among chop locations (A = 0.31, kg B = 0.24 kg, C = 0.24 kg, and D = 0.23 kg). These results demonstrated that variability in tenderness values did not reflect the variability of IMF. In conclusion, chop location, sex, and sire line all contribute to the amount and variability of pork loin marbling.
Relative bioavailability of guanidinoacetic acid delivered ruminally or abomasally to cattle
Authors: Speer Hannah F , Pearl Kimberly A , Titgemeyer Evan C 
Published: August 2020

AbstractThis study assessed the relative bioavailability of guanidinoacetic acid (GAA) in cattle. Seven ruminally cannulated Holstein steers (initial body weight of 280 kg) were used in an experiment with a 5 × 5 Latin square design; the two additional steers received a treatment sequence identical to two steers in the Latin square. Treatments were: control (no GAA, water infusion), ruminal infusion of 10 or 20 g/d GAA, and abomasal infusion of 10 or 20 g/d GAA, with all infusions delivered continuously. Periods were 7 d in length, and on day 7, blood and urine samples were collected to determine the concentrations of GAA and its associated metabolites. Plasma creatine concentrations increased linearly (P &lt; 0.01) with GAA infusion to the abomasum and tended to increase linearly (P = 0.06) when GAA was infused ruminally. Urinary creatine concentrations increased linearly with increasing amounts of GAA infused in the abomasum (P &lt; 0.01) and the rumen (P &lt; 0.05). There were no significant effects of GAA infusion to either the abomasum or rumen on plasma or urinary concentrations of GAA. Plasma creatinine concentrations were not affected by GAA infusion to the abomasum or rumen. Urinary creatinine concentrations decreased when GAA was infused abomasally (P &lt; 0.05). Because plasma and urinary creatine concentrations yielded the statistically strongest linear responses, they were selected as the primary response criteria for quantifying ruminal escape of GAA. Calculated by slope-ratio methodology, estimates for the ruminal escape of GAA based on plasma creatine and urinary creatine concentrations were 47% and 49%, respectively. Ruminally infused GAA was about half as effective as abomasally infused GAA in elevating plasma and urinary concentrations of creatine.
Comparison of in Vitro and in Planta Toxicity of Vip3A for Lepidopteran Herbivores
Authors: Khan Muhammad Hassaan , Jander Georg , Mukhtar Zahid , Arshad Muhammad , Sarwar Muhammad , Asad Shaheen 
Published: August 2020

AbstractAgricultural pest infestation is as old as domestication of food crops and contributes a major share to the cost of crop production. In a transgenic pest control approach, plant production of Vip3A, an insecticidal protein from Bacillus thuringiensis, is effective against lepidopteran pests. A synthetic Vip3A gene was evaluated for efficacy against Spodoptera litura Fabricius (Lepidoptera: Noctuidae; cotton leafworm), Spodoptera exigua Hübner (Lepidoptera: Noctuidae; beet armyworm), Spodoptera frugiperda Smith (Lepidoptera: Noctuidae; fall armyworm), Helicoverpa armigera Hübner (Lepidoptera: Noctuidae; cotton bollworm), Helicoverpa zea Boddie (Lepidoptera: Noctuidae; corn earworm), Heliothis virescens Fabricius (Lepidoptera: Noctuidae; tobacco budworm), and Manduca sexta L. (Lepidoptera: Sphingidae; tobacco hornworm) in tobacco. In artificial diet assays, the concentration required to achieve 50% mortality was highest for H. zea followed by H. virescens &gt; S. exigua &gt; H. armigera &gt; M. sexta &gt; S. frugiperda &gt; S. litura. By contrast, in bioassays with detached leaves from Vip3A transgenic tobacco, the time until 50% lethality was M. sexta &gt; H. virescens &gt; S. litura &gt; H. zea &gt; H. armigera &gt; S. exigua. There was no significant correlation between the artificial diet and transgenic plant bioassay results. Notably, the two insect species that are best-adapted for growth on tobacco, M. sexta and H. virescens, showed the greatest time to 50% mortality on Vip3A-transgenic tobacco. Together, our results suggest that artificial diet assays may be a poor predictor of Vip3A efficacy in transgenic plants, lepidopteran species vary in their sensitivity to Vip3A in diet-dependent manner, and host plant adaptation of the targeted herbivores should be considered when designing transgenic plants for pest control.
Inheritance and Fitness Costs of Cry3Bb1 Resistance in Diapausing Field Strains of Western Corn Rootworm (Coleoptera: Chrysomelidae)
Authors: Shrestha Ram B , Gassmann Aaron J 
Published: August 2020

Abstract
Field-evolved resistance to Cry3Bb1 corn by western corn rootworm, Diabrotica virgifera virgifera LeConte (Colleoptera: Chrysomellidae), has been reported in field populations in Iowa, Illinois, Nebraska, and Minnesota. Inheritance and fitness costs associated with Cry3Bb1 resistance have been determined for non-diapausing laboratory strains of western corn rootworm with either laboratory-selected resistance or field-derived resistance. However, information on inheritance and fitness costs of Cry3Bb1 resistance in the diapausing field populations is lacking. In this study, we determined the inheritance of Cry3Bb1 resistance for four diapausing field strains of western corn rootworm using plant-based bioassays. We also determined the fitness costs for eight diapausing field populations in a greenhouse experiment. We found that Cry3Bb1 resistance was an autosomal trait and that the inheritance of resistance was mostly non-recessive; however, there was some variation in the dominance of Cry3Bb1 resistance. We did not find evidence of fitness costs affecting survival to adulthood, developmental rate, or adult dry mass. However, we did detect a fitness cost affecting adult size. The results of this study will add to the current understanding of field-evolved resistance to Cry3Bb1 corn by western corn rootworm and help in developing better strategies to manage resistance.
Bladder preservation therapy for muscle invasive bladder cancer: the past, present and future
Authors: Kimura Tomokazu , Ishikawa Hitoshi , Kojima Takahiro , Kandori Shuya , Kawahara Takashi , Sekino Yuta , Sakurai Hideyuki , Nishiyama Hiroyuki 
Published: August 2020

AbstractRadical cystectomy is the gold standard treatment for muscle invasive bladder cancer, but some patients have medically inoperable disease or refuse cystectomy to preserve their bladder function. Bladder preservation therapy with transurethral resection of the bladder tumor and concurrent chemoradiotherapy, known as trimodal treatment, is regarded to be a curative-intent alternative to radical cystectomy for patients with muscle invasive bladder cancer during the past decade. After the development of immune checkpoint inhibitors, a world-changing breakthrough occurred in the field of metastatic urothelial carcinoma and many clinical trials have been conducted against non-muscle invasive bladder cancer. Interestingly, preclinical and clinical studies against other malignancies have shown that immune checkpoint inhibitors interact with the radiation-induced immune reaction. As half of the patients with muscle invasive bladder cancer are elderly, and some have renal dysfunction, not only as comorbidity but also because of hydronephrosis caused by their tumors, immune checkpoint inhibitors are expected to become part of a new therapeutic approach for combination treatment with radiotherapy. Accordingly, clinical trials testing immune checkpoint inhibitors have been initiated to preserve bladder for muscle invasive bladder cancer patients using radiation and immune checkpoint inhibitors with/without chemotherapy. The objective of this review is to summarize the evidence of trimodal therapy for muscle invasive bladder cancer during the past decade and to discuss the future directions of bladder preservation therapy in immuno-oncology era.
Feasibility study of cryobiopsy for practical pathological diagnosis of primary lung cancer including immunohistochemical assessment
Authors: Nishida Tomoki , Matsumoto Yuji , Sasada Shinji , Tanaka Midori , Nakai Toshiyuki , Fukai Ryuta , Ohe Yuichiro , Watanabe Shun-ichi , Motoi Noriko 
Published: August 2020

AbstractBackgroundPrecision medicine in non-small cell lung cancer requires attainment of a sufficient amount of high-quality tumor tissue. Transbronchial cryobiopsy has emerged as a new diagnostic method for non-neoplastic lung disease with a better potential to assess morphology compared with conventional methods. However, the influence of cryobiopsy on specimen quality, particularly detection of protein expression, is unknown. We performed a comparative immunohistochemical study in specimens obtained by cryobiopsy versus conventional sampling to evaluate the feasibility of cryobiopsy for lung cancer diagnosis.MethodsPairs of artificial biopsy specimens, collected using a cryoprobe or conventional scalpel, were obtained from 43 surgically resected primary lung tumors. Formalin-fixed, paraffin-embedded blocks were prepared in an ISO15189-certified laboratory. Immunohistochemical staining of thyroid transcription factor-1, p40, Ki67 and programmed death-ligand 1 (22C3) was performed. The H-scores for thyroid transcription factor-1 and p40, labeling index for Ki67 and tumor proportion score for programmed death-ligand 1 were assessed. Pearson’s correlation coefficients between two sampling types were calculated.ResultsThe thyroid transcription factor-1 and p40 H-scores showed perfect correlations between the cryobiopsy and conventional scalpel-obtained specimens (R2 = 0.977 and 0.996, respectively). Ki67 labeling index and PD-L1 tumor proportion score also showed strong correlations between the two sample types (R2 = 0.896 and 0.851, respectively). Five cases (11.6%) exhibited differences in tumor proportion score category between sample types, potentially because of intratumoral heterogeneity.ConclusionsImmunohistochemical expression of certain tumor markers showed a high concordance between cryobiopsy and conventional scalpel sampling. Cryobiopsy is feasible for pathological diagnostics including PD-L1 evaluation.
Genome-Wide Association Study of Susceptibility Loci for TCF3-PBX1 Acute Lymphoblastic Leukemia in Children
Authors: Lee Shawn H. R , Qian Maoxiang , Yang Wentao , Diedrich Jonathan D , Raetz Elizabeth , Yang Wenjian , Dong Qian , Devidas Meenakshi , Pei Deqing , Yeoh Allen , Cheng Cheng , Pui Ching-Hon , Evans William E , Mullighan Charles G , Hunger Stephen P , Savic Daniel , Relling Mary V , Loh Mignon L , Yang Jun J 
Published: August 2020

Abstract
Acute lymphoblastic leukemia (ALL) is the most common cancer in children. TCF3-PBX1 fusion defines a common molecular subtype of ALL with unique clinical features, but the molecular basis of its inherited susceptibility is unknown. In a genome-wide association study of 1494 ALL cases and 2057 non-ALL controls, we identified a germline risk locus located in an intergenic region between BCL11A and PAPOLG: rs2665658, P = 1.88 × 10–8 for TCF3-PBX1 ALL vs non-ALL, and P = 1.70 × 10–8 for TCF3-PBX1 ALL vs other-ALL. The lead variant was validated in a replication cohort, and conditional analyses pointed to a single causal variant with subtype-specific effect. The risk variant is located in a regulatory DNA element uniquely activated in ALL cells with the TCF3-PBX1 fusion and may distally modulate the transcription of the adjacent gene REL. Our results expand the understanding of subtype-specific ALL susceptibility and highlight plausible interplay between germline variants and somatic genomic abnormalities in ALL pathogenesis.
“I Think It’s Been Met With a Shrug:” Oncologists’ Views Toward and Experiences With Right-to-Try
Authors: Smith Cambray , Stout Jeremiah , Adjei Alex A , Buckner Jan , Wentworth Mark , Tilburt Jon , Master Zubin 
Published: August 2020

Abstract

Background
The federal Right-to-Try (RTT) Act created an alternate regulatory pathway for preapproval access to investigational drugs. A few studies have examined the experiences of physicians with the Food and Drug Administration’s Expanded Access Programs, but to our knowledge, no study has yet to examine their attitudes and experiences toward RTT.

Methods
This study explored the views of 21 oncologists at a major cancer center with 3 main sites across the United States using semi-structured interviews and qualitative analysis. Participants were selected to have experience with Expanded Access Programs.

Results
Most oncologists had limited familiarity with RTT, and several reported confusion about the legislation, including whether patients have a right to investigational drugs and an obligation for companies to provide them. Although oncologists were interested in decreased regulatory burdens, 3 areas of concern were articulated: lack of safety and oversight, unclear structure and no provision for data collection, and potential heightening of patient expectations. Only 4 oncologists had experience discussing RTT, and none formally attempted to obtain the drug through this mechanism. Participants questioned the practicality of RTT legislation and suggested alternative ways to improve access.

Conclusions
The study provides foundational empirical data underlying challenging ambiguities by experienced oncologists familiar with off-trial use of investigational therapeutics and reaffirms the role of physicians and regulatory bodies in mitigating the risks of investigational drugs. Our findings highlight the need for medical centers to inform oncologists about RTT and other preapproval pathways so that they are able to address questions from patients interested in nontrial investigational drugs.
Health, Integration and Agency: Sport Participation Experiences of Asylum Seekers
Authors: Ley Clemens , Karus Felix , Wiesbauer Lisa , Rato Barrio María , Spaaij Ramon 
Published: August 2020

Abstract
Politicians, scholars, and practitioners have drawn attention to social and health benefits of sport participation in the context of forced migration and refugee settlement. This study aims to progress conceptual and practical understandings of how asylum seekers’ past and present experiences shape their sport participation. We present an instrumental case study drawn from the Movi Kune programme to discuss the experiences of an asylum seeker holistically, in a particular context in time and space. The findings illustrate how pre-migration, migratory, and present experiences of living in prolonged uncertainty and liminality all strongly affect sport participation and its health and integration outcomes. The results further show that sport participation was an opportunity to perform agency, experience mastery, coping, and social recognition, promoting positive self-efficacy beliefs, health and social connection over time. Our findings extend the literature by indicating that sport practices can enhance human agency to cope with health issues and distressing past and present experiences during the asylum-seeking process.
The synchrotron polarization in decaying magnetic field in gamma-ray bursts
Authors: Cheng K F , Zhao X H , Bai J M 
Published: August 2020

ABSTRACTPolarization can serve as a probe of the radiation mechanism and magnetic field (MF) configuration in gamma-ray bursts (GRBs). In the case of constant MF, the synchrotron polarization in the prompt phase of GRBs has been widely studied. In this paper, we consider the case of the decaying MF. We calculate the time-averaged and instantaneous synchrotron polarizations in a pulse for different viewing angles and for the large-scale decaying MF model, which can explain the so-called Band spectrum. We find that the on-axis time-averaged polarization degree (PD) in the energy band of 50–500 keV for the decaying large-scale MF model (∼0.6 for typical parameters) is higher than that in the constant MF model (∼0.5). An interesting result is the instantaneous PD in the off-axis case will experience a turnover, i.e. the PD will evolve from a positive value to a negative one. This suggests the polarization angle (PA) change by an angle of 90°. Such a result is roughly consistent with the discovery of the PA evolution within a pulse in some bursts, such as GRB 170114A and GRB 160821A. Our result implies at least a part of bursts (off-axis bursts) should have the PA evolution in a pulse.
The observational impact of dust trapping in self-gravitating discs
Authors: Cadman James , Hall Cassandra , Rice Ken , Harries Tim J , Klaassen Pamela D 
Published: August 2020

ABSTRACT
We present a 3D semi-analytical model of self-gravitating discs, and include a prescription for dust trapping in the disc spiral arms. Using Monte Carlo radiative transfer, we produce synthetic ALMA (Atacama Large Millimeter/submillimeter Array) observations of these discs. In doing so, we demonstrate that our model is capable of producing observational predictions, and able to model real image data of potentially self-gravitating discs. For a disc to generate spiral structure that would be observable with ALMA requires that the disc’s dust mass budget is dominated by millimetre- and centimetre-sized grains. Discs in which grains have grown to the grain fragmentation threshold may satisfy this criterion; thus, we predict that signatures of gravitational instability may be detectable in discs of lower mass than has previously been suggested. For example, we find that discs with disc-to-star mass ratios as low as 0.10 are capable of driving observable spiral arms. Substructure becomes challenging to detect in discs where no grain growth has occurred or in which grain growth has proceeded well beyond the grain fragmentation threshold. We demonstrate how we can use our model to retrieve information about dust trapping and grain growth through multiwavelength observations of discs, and using estimates of the opacity spectral index. Applying our disc model to the Elias 27, WaOph 6, and IM Lup systems, we find gravitational instability to be a plausible explanation for the observed substructure in all three discs, if sufficient grain growth has indeed occurred.
Opening reionization: quantitative morphology of the epoch of reionization and its connection to the cosmic density field
Authors: Busch Philipp , Eide Marius B , Ciardi Benedetta , Kakiichi Koki 
Published: August 2020

ABSTRACT
We introduce a versatile and spatially resolved morphological characterization of binary fields, rooted in the opening transform of mathematical morphology. We subsequently apply it to the thresholded ionization field in simulations of cosmic reionization and study the morphology of ionized regions. We find that an ionized volume element typically resides in an ionized region with radius ∼8 h−1 cMpc at the midpoint of reionization (z ≈ 7.5) and follow the bubble size distribution even beyond the overlap phase. We find that percolation of the fully ionized component sets in when 25 per cent of the universe is ionized and that the resulting infinite cluster incorporates all ionized regions above ∼8 h−1 cMpc. We also quantify the clustering of ionized regions of varying radius with respect to matter and on small scales detect the formation of superbubbles in the overlap phase. On large scales, we quantify the bias values of the centres of ionized and neutral regions of different sizes and not only show that the largest ones at the high-point of reionization can reach b ≈ 30, but also that early small ionized regions are positively correlated with matter and large neutral regions and late small ionized regions are heavily antibiased with respect to matter, down to b ≲ −20.
Observability of dusty debris discs around M-stars
Authors: Luppe Patricia , Krivov Alexander V , Booth Mark , Lestrade Jean-François 
Published: August 2020

ABSTRACT
Debris discs are second-generation dusty discs formed by collisions of planetesimals. Many debris discs have been found and resolved around hot and solar-type stars. However, only a handful have been discovered around M-stars, and the reasons for their paucity remain unclear. Here, we check whether the sensitivity and wavelength coverage of present-day telescopes are simply unfavourable for detection of these discs or if they are truly rare. We approach this question by looking at the Herschel/DEBRIS survey that has searched for debris discs including M-type stars. Assuming that these cool-star discs are ‘similar’ to those of the hotter stars in some sense (i.e. in terms of dust location, temperature, fractional luminosity, or mass), we check whether this survey should have found them. With our procedure we can reproduce the $2.1^{+4.5}_{-1.7}$ per cent detection rate of M-star debris discs of the DEBRIS survey, which implies that these discs can indeed be similar to discs around hotter stars and just avoid detection. We then apply this procedure to IRAM NIKA-2 and ALMA bands 3, 6, and 7 to predict possible detection rates and give recommendations for future observations. We do not favour observing with IRAM, since it leads to detection rates lower than for the DEBRIS survey, with 0.6–4.5 per cent for a 15 min observation. ALMA observations, with detection rates 0.9–7.2 per cent, do not offer a significant improvement either, and so we conclude that more sensitive far-infrared and single dish sub-millimetre telescopes are necessary to discover the missing population of M-star debris discs.