bluuebunny/crossref_metadata_2025_split huggingface dataset:
Health Risk, Functional Markers and Cognitive Status in Institutionalized Older Adults: A Longitudinal Study
Authors: Pedrero-Chamizo Raquel , Albers Ulrike , Palacios Gonzalo , Pietrzik Klaus , Meléndez Agustín , González-Gross Marcela 
Published: July 2020

Abstract

Background
Follow-up of vitamin B12 and lipids status is essential in older people, being closely related to non-communicable diseases. Their relationships with cognitive and physical status are not clear. The purpose of this study was to analyze the evolution of vitamin B12 and related parameters, lipid and hematological profiles, and their relationships with cognitive and physical status among institutionalized elderly.
Methods
Sixty residents (68% women), ranged from 62 to 99 years (mean age 85.1 ± 5.5 and 80.6 ± 9.9 years for women and men, respectively) were recruited from three nursing homes in Madrid Community (Spain). Biomarkers (vitamin B12 and related parameters, lipid and hematological profiles), functional capacity (handgrip, arm and leg strength), and cognitive status (Mini-Mental State Examination) were evaluated four times at 3-month intervals.
Results
At the beginning of the study, 63% and 70% of the sample showed abnormal homocysteine and folate values, respectively. At the end of the year, abnormal homocysteine increased to 68%, abnormal folate values decreased to 50%. Throughout the year, serum folate showed a significant increase (14.9 vs. 16.3 nmol/L), (p < 0.05). Serum cobalamin (299 vs. 273 pmol/L). HDL-cholesterol (49.9 vs. 47.0 mg/dL) and triglyceride levels (102.4 vs. 123.2 mg/dL) showed a significant decrease and increase respectively in mean values (all p < 0.05). Serum cobalamin and HDL-cholesterol were the most important biomarkers associated with cognitive function (both p < 0.05). The most relevant biomarkers associated with poor physical strength depending on the body part analyzed were low concentrations of HDL-cholesterol, LDL-cholesterol, apolipoprotein A1, and albumin (all p < 0.05).
Conclusions
The evolution of lipid biomarkers, their significance with cognitive values, and association with handgrip, point to the importance of the handgrip measurement, a very simple test, as an important health marker. Both serum albumin and physical strength are important health markers in older people.
Chiaurinib Selectively Inhibits Colorectal Cancer with KRAS Wild-Type by Modulation of ROS through Activating the p53 Signaling Pathway
Authors: Yin Haofan , Xie Jineye , Jiang Ping , Jiang Xi , Duan Deyu , Qi Junhua , Ma Caiqi , Hong Honghai , Luo Zhaofan 
Published: July 2020

Abstract
Background: Colorectal cancer (CRC) is one of the top three most deadly cancers despite using chemotherapy based on oxaliplatin or irinotecan combined with targeted therapy. Chiaurinib has recently been identified to be a promising anticancer candidate with impressive efficacy and safety. However, the role and molecular mechanisms of Chiaurinib in the treatment of CRC remain to be elucidated.Methods: Cell proliferation and apoptosis were detected by CCK-8, EDU staining, Colony formation assay, TUNEL staining and flow cytometric analysis. ROS production was confirmed by Mito-SOX and DCF-DA fluorescence. RNA-Seq and GSEA analysis were used to explore the mechanisms of the effect of Chiaurinib in KRAS wild-type CRC cells.Results: Our study shows that Chiaurinib inhibits cell proliferation and induces apoptosis in KRAS wild-type CRC cells in a dose- and time-dependent manner, but not mutation ones. Meanwhile, Chiaurinib increases ROS production in KRAS wild-type CRC cells. Moreover, Chiaurinib selectively suppresses KRAS wild-type CRC cells growth in vivo. Mechanistically, Chiaurinib inhibits KRAS wild-type CRC cells by triggering ROS production via activating the p53 signaling pathway. Further, KRAS mutation CRC cells are resistant to Chiaurinib by increasing Nrf2 to stably elevate the basal antioxidant program and thereby lower intracellular ROS induced by Chiaurinib.Conclusions: Taken together, we reveal that Chiauranib induces p53 upregulation, resulting in ROS accumulation, thus inhibiting cell proliferation and inducing apoptosis in KRAS wild-type CRC cells. Our findings provide the rationale for further clinical evaluation of Chiaurinib as a therapeutic agent in treating KRAS wild-type CRC.
The potential identical hemo-biological makers to the left abomasal displacement in dairy cows
Authors: Song Yuxiang , Loor Juan J , Zhao Chenchen , Huang Dan , Du Xiliang , Li Xiaobing , Wang Zhe , Liu Guowen , Li Xinwei 
Published: July 2020

Abstract
Background
Left abomasal displacement (LDA) occurs at high frequency in the early postpartum period and can affect production performance of dairy cows. Clinical diagnosis of LDA is usually done by abdominal auscultation and percussion. The purpose of this study was to explore the potential applicability of blood biomarkers for early warning and diagnosis of LDA in dairy cows.
Results
Twenty early postpartum healthy cows and thirty early postpartum LDA cows of similar parity were used. A receiver operating characteristic curve (ROC) method was used to analyze the sensitivity of hematological biomarkers to LDA including energy balance metabolic biomarkers, liver/renal function biomarkers, and minerals. A cut-off point was defined for each of the selected hematological biomarkers deemed sensitive markers of LDA. Compared with healthy cows, body condition score (BCS), dry matter intake (DMI) and milk production were lower in LDA cows. Among energy metabolism markers, serum non-esterified fatty acid (NEFA), β-hydroxybutyric acid (BHBA), insulin (INS), and revised quantitative insulin sensitivity check index (RQUICKI) levels were lower while serum glucagon (GC) was greater in LDA cows. Among the liver/renal function biomarkers, activities of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), γ-glutamyl transpeptidase (GGT), lactate dehydrogenase (LDH), the ratio of AST/ALT and levels of total bilirubin (TBIL), direct bilirubin (DBIL), indirect bilirubin (IBIL), albumin (ALB), blood urea nitrogen (BUN), creatinine (CREA), and total protein (TP) were greater in LDA cows. Among minerals analyzed, serum Cl, Ca, and K were lower in LDA cows. After ROC analysis, it was determined that serum Ca, INS, RQUICKI, ALT, GGT, and CREA are potential indicators for early warning and diagnosis of LDA in dairy cows.
Conclusions
Dairy cows with LDA were under severe negative energy balance (NEB), had signs of liver damage and potentially lower insulin sensitivity. A combination of multi-hematological biomarkers including Ca, INS, RQUICKI, ALT, GGT and CREA has the potential to help identify cows at risk of LDA.
Predicting the Effect of Environmental Humidity on COVID-19 Trend in Medan, Indonesia
Authors: Purnama Tri Bayu 
Published: July 2020

Abstract
COVID-19 remained a public health problem and reportedly associated with the environment. However, this environment parameters have not determined yet the correlation to COVID-19. This study used ecological study to investigate time series of environment parameters and COVID-19 data. Spearman correlation test was applied to analyzed the correlation. Humidity has detected as positive correlation with COVID-19 and temperature has shown as negative correlation. Predicting trend of environment parameters and COVID-19 is needed to conduct public health preparedness of COVID-19 strategy.
Clinical Performance of Non-Invasive Prenatal Testing for Sex Chromosomal Aneuploidies in Northeast China
Authors: Wang Lu , Dai Rulin , Shi Qingyang , Jiang Yuting , Zhang Hongguo , Liu Ruizhi , Xi Qi 
Published: July 2020

Abstract
Background: Along with the discovery of cell-free DNA (cfDNA) and the invention of next-generation sequencing (NGS), non-invasive prenatal testing (NIPT) had appeared and been applied for detecting common aneuploidies such as trisomy 21, 18, and 13, with low false-negative and false-positive rates. Recently, it had also been used for sex chromosome aneuploidies (SCAs). To assess the clinical utility of NIPT for SCAs in Northeast China, we collected NIPT data from BGI 500 sequencing platform in the Center for Reproductive Medicine, Center for Prenatal Diagnosis of the First Hospital of Jilin University, and calculate the positive predictive value (PPV) and false positive rate (FPR). Results: A cohort of 14936 samples were analyzed by NIPT, and revealed 70 cases with SCAs high-risk, among them, 40 women agreed to undergo amniocentesis, but as many as 30 ones refused further diagnose. Based on verified fetal karyotype, 30.0% (12/40) were confirmed to be a true positive. Unluckily, the PPV for monosomy X performed 0%. Besides, positive 47,XXX were 46.67% (7/15), 40.00% (2/5) were positive for 47, XYY, and 42.86% (3/7) were positive for 47, XXY.Conclusions: In conclusion, our present results confirmed that NIPT sequenced by BGI 500 demonstrated lowest PPVs for 45,X, but the more accurate prediction for other SCAs, it is still a potential method for SCAs screening. Henceforth, we should focus on how to improve the test utility and provide better services for pregnant women in need.
Special Foods and Local Herbs Used to Enhance Breast milk Production in Ghana: Rate of Use and Beliefs of Efficacy
Authors: Ali Zakari , Bukari Mohammed , Mwinisonaam Anita , Abdul-Rahaman Abdul-Latif , Abizari Abdul-Razak 
Published: July 2020

Abstract
Background
Inadequate breast milk production is one of the key factors associated with suboptimal breastfeeding. In most local African homes, special herbs and some food items are commonly used to promote breast milk production (known as lactogogue/galactogogue). We describe the use and characterize the herbs and food items used to promote breastmilk production in two regions of Ghana.
Methods
We conducted a cross-sectional study involving 402 lactating mothers. The range of foods used as lactogogues was obtained through focus group discussions. Quantitative data on demographics, lactogogue use and feeding practices were obtained through questionnaire administration.
Results
The mean age of women was 29.2 years and children were 10 months. Breastmilk production problems were low (22.4%) and majority of lactating mothers felt they had adequate breastmilk (70.4%) but awareness about lactogogues was widespread in both regions (88.8%) and highest in the Brong-Ahafo region (90.0%). Information about lactogogues was mainly from grandparents (24.6%), parents (31.6), health facilities (16.5%) and friends (12.8%), while the media had little influence (< 1%). Prevalence of lactogogue use was 83.8%, lactogogues were prepared separate from household meals (59.4%) and consumed 1 to 3 times a day (89.6%). Users felt the effectiveness within 24hrs of use (98.5%). The most common lactogogues included; groundnut/peanut soup prepared with Bra leaves (Hibiscus sabdariffa), hot black tea, Werewere/Agushi (Citrulus colocynthis) prepared with Bra leaves, and Abemudro (a polyherbal formulation). Only 13.2% of lactating mothers also used lactogogues during pregnancy.
Conclusion
Special foods and selected herbs are widely used to enhance breastmilk production in Ghana and constitute an important part of the diet of lactating mothers. These results could contribute to understanding breastfeeding behaviours and professional support for lactating women with breast milk production concerns in Ghana.
WITHDRAWN: A first Japanese Case of Intraductal Cancer of the Prostate with CHEK2 Mutation
Authors: Hongo Hiroshi , Kosaka Takeo , Nakamura Kohei , Mikami Shuji , Nishihara Hiroshi , Oya Mototsugu 
Published: July 2020

Abstract
Background: Genomic profiling of tissue biopsies can be used to investigate genomic alterations. Although genomic analysis is also useful for precision medicine of prostate cancer, it is unclear whether to apply the results of this analysis to treatment. Case presentation: A 62-year-old man underwent docetaxel therapy and enzalutamide for castration-resistant prostate cancer. However, he developed urinary retention and radiological images showed progression of prostate cancer. We performed prostate biopsy and trans-urethral resection of the prostate. Pathological examination showed acinar adenocarcinoma with intraductal carcinoma. Although docetaxel rechallenge and cabazitaxel therapy were attempted, the tumor expanded to the rectum and his systemic condition worsened gradually (Fig. 1). Palliative care was started and the patient was transferred to another hospital. We performed next-generation sequencing using DNA from FFPE tissue of the TUR-P specimen. We identified 4 genetic variants with in the FFPE tissue sample. Remarkably, CHEK2 alteration was known to be associated with some solid cancer progression. Conclusions: The results of genetic analysis suggested that modification of tumor suppressor pathways might be associated with refractory nature of his cancer.
Social Distancing During the COVID-19 Pandemic: Quantifying the Practice in Michigan – a “Hotspot State” Early in the Pandemic – Using a Volunteer-Based Online Survey
Authors: Cassidy-Bushrow Andrea E. , Baseer Mohammed , Kippen Karen , Levin Albert M. , Li Jia , Loveless Ian , Poisson Laila M. , Schultz Lonni , Wegienka Ganesa , Zhou Yueren , Johnson Christine Cole 
Published: July 2020

Abstract
Background: Public Health policies related to social distancing efforts during the COVID-19 pandemic helped slow the infection rate. However, individual-level factors associated with social distancing are largely unknown. We sought to examine social distancing during the COVID-19 pandemic in Michigan, an infection “hotspot” state in the United States early in the pandemic.Methods: Two surveys were distributed to Michigan residents via email lists and social media following COVID-19 related state mandates in March; 45,691 adults responded to the first survey and 8,512 to the second. Staying home ≥ 3 out of 5 previous days defined having more social distancing. Logistic regression models were used to examine potential factors associated with more social distancing.Results: Most respondents were women (86% in Survey 1, 87% in Survey 2). In Survey 1, 63% reported more social distancing, increasing to 78% in Survey 2. Female sex and having someone (or self) sick in the home were consistently associated with higher social distancing, while increasing age was positively associated in Survey 1 but negatively associated in Survey 2. Most respondents felt social distancing policies were important (88% in Survey 1; 91% in Survey 2). Conclusions: Michiganders responding to the surveys were both practicing and supportive of social distancing. State-level executive orders positively impacted behaviors early in the COVID-19 pandemic in Michigan. Additional supports are needed to help vulnerable populations practice social distancing, including older individuals.
Inorganic contaminants in Canadian First Nation community water systems
Authors: Lane Kaycie , Trueman Benjamin F. , Locsin Javier , Gagnon Graham A. 
Published: July 2020

AbstractWhile previous Canadian studies have examined microbiological water quality in First Nations, there is little published information on inorganic contaminants. In Atlantic Canada, the lead, manganese, and arsenic content of First Nations’ drinking water has been measured for more than a decade, but the data have not been analyzed comprehensively. These contaminants are linked with health problems, and high levels in drinking water are a cause for concern. We examined 12 years of data from 47 First Nation community water systems to identify systems experiencing difficulties meeting sampling frequency or regulatory guidelines. While most contaminant concentrations were below guideline values, we identified elevated concentrations and issues with sampling frequency. No system met both sampling frequency requirements – a minimum of one sample per year per analyte – and regulatory guidelines. Exceedance rates for lead, manganese, and arsenic were high in some systems. Moreover, current sampling procedures for lead specify that taps be flushed prior to sampling, which is known to underestimate lead exposure. We find that a switch to random daytime sampling would at least sometimes yield higher estimates of lead at the tap. Our analysis demonstrates the need for increased monitoring and updated sampling procedures to better characterize inorganic contaminant occurrence in First Nations.
Tertiary phosphorus removal to extremely low levels by coagulation-flocculation and cloth-filtration
Authors: Fundneider T. , Alejo L. , Lackner S. 
Published: July 2020

Abstract
Higher standards in the European Water Framework Directive and national directive demand advanced wastewater treatment for removal of nutrients and organic micropollutants before the discharge into water bodies. Systematic investigations regarding relative dosage and filtration processes for removal of flocculated solids are currently lacking. In this study, the performance of technologies for advanced removal of total phosphorus down to &lt;100 μg/L with pile cloth-filtration (CF) and membrane filtration was verified and synergy effects for the removal of other contaminants were identified. The results show that an over-stoichiometric addition of coagulants of &gt;5 mol Me3+/mol sRP was necessary to achieve soluble reactive phosphorus (sRP) concentrations of &lt;50 μg/L in the effluent. After the coupled process of tertiary phosphorus removal and solids removal, the soluble non-reactive phosphorus (sNRP) concentration regulates the lowest total phosphorus effluent concentration. sNRP is also partially, but not completely, removed by the use of coagulants. CF has proven to be an alternative technology for the removal of phosphorus and total suspended solids below the detection limit.
Proapoptotic Effects of triazol-1,4-Naphthoquinones Involve Intracellular ROS Production and MAPK/ERK Pathway in Human Leukemia Cells
Authors: Coulidiati Tangbadioa H. , Dantas Bruna B. , Faheina-Martins Glaucia V. , de Morais Gomes Enéas Ricardo , Gonçalves Juan C.R. , de Araújo Demetrius A. Machado 
Published: July 2020

Background:
The natural products constitute an important source of antitumor and cytotoxic agents.
Naphthoquinones are effectively quinones present in different plants, with demonstrated anticancer activities. A
recent study conducted by our group demonstrated the antileukemic potential of two novel triazol-1,4-
naphthoquinones derivatives, PTN (2-(4-Phenyl-1H-1,2,3-triazol-1-yl)-1,4-naphthoquinone) and MPTN (2-[4-
(4-Methoxyphenyl)-1H-1,2,3-triazol-1-yl]-1,4-naphthoquinone). Although, the mechanisms underlying the
proapoptotic effects of PTN and MPTN have not been fully elucidated so far.

Objective:
The aim of this study was to evaluate the proapoptotic mechanism of PTN and MPTN in human
acute leukemia cells.

Methods:
We used fluorescence microscopy to observe acridine orange and annexin V staining cells. Flow
cytometry assay has also been used for ROS quantification, BAX and cytochrome c proteins expression and
apoptosis analysis. MTT assay and western blotting technique have been performed as well for MAPK pathway
analysis.

Results:
By using the acridine orange and annexin V staining with fluorescence microscopy, we have characterized
the proapoptotic effects of PTN and MPTN in HL-60 cells involving the intrinsic mitochondrial pathway,
since these compounds promoted an increase in the intracellular BAX and cytochrome c protein levels (p<0.05).
We further demonstrated that apoptosis induction in HL-60 cells was mediated by increasing intracellular ROS
levels via ERK but not p38 MAPKs pathway.

Conclusion:
Taken together, these results have demonstrated that PTN and MPTN are promising tools for the
development of new anti-leukemic drugs.
lncRNAs as Potential Targets in Small Cell Lung Cancer: MYC -dependent Regulation
Authors: Tokgun Onur , Tokgun Pervin E. , Inci Kubilay , Akca Hakan 
Published: July 2020

Background:
Small Cell Lung Cancer (SCLC) is a highly aggressive malignancy. MYC family oncogenes
are amplified and overexpressed in 20% of SCLCs, showing that MYC oncogenes and MYC regulated
genes are strong candidates as therapeutic targets for SCLC. c-MYC plays a fundamental role in cancer stem
cell properties and malignant transformation. Several targets have been identified by the activation/repression of
MYC. Deregulated expression levels of lncRNAs have also been observed in many cancers.

Objective:
The aim of the present study is to investigate the lncRNA profiles which depend on MYC expression
levels in SCLC.

Methods:
Firstly, we constructed lentiviral vectors for MYC overexpression/inhibition. MYC expression is
suppressed by lentiviral shRNA vector in MYC amplified H82 and N417 cells, and overexpressed by lentiviral
inducible overexpression vector in MYC non-amplified H345 cells. LncRNA cDNA is transcribed from total
RNA samples, and 91 lncRNAs are evaluated by qRT-PCR.

Results:
We observed that N417, H82 and H345 cells require MYC for their growth. Besides, MYC is not only
found to regulate the expressions of genes related to invasion, stem cell properties, apoptosis and cell cycle (p21,
Bcl2, cyclinD1, Sox2, Aldh1a1, and N-Cadherin), but also found to regulate lncRNAs. With this respect, expressions
of AK23948, ANRIL, E2F4AS, GAS5, MEG3, H19, L1PA16, SFMBT2, ZEB2NAT, HOTAIR,
Sox2OT, PVT1, and BC200 were observed to be in parallel with MYC expression, whereas expressions of
Malat1, PTENP1, Neat1, UCA1, SNHG3, and SNHG6 were inversely correlated.

Conclusion:
Targeting MYC-regulated genes as a therapeutic strategy can be important for SCLC therapy. This
study indicated the importance of identifying MYC-regulated lncRNAs and that these can be utilized to develop
a therapeutic strategy for SCLC.