bluuebunny/crossref_metadata_2025_split huggingface dataset:
THE EFFECT OF DECELLULARIZATION TECHNIQUE ON COLLAGEN TYPE II AND MATRICES POROSITY OF CARTILAGE BOVINE SCAFFOLD
Authors: Utomo Dwikora Novembri , Sari Ika Benny Kartika 
Published: February 2020

Background: Cartilage has limited capacity for self-repair in case of defect. Stem cell based tissue engineering has become an alternative therapy for the treatment of cartilage defect. The use of decellularized cartilage bovine can serve as scaffold to support proliferation dan differentiation of the stem cell.Purpose: This research is to compare the effect of decellularization technique on collagen type II and matrices porosity of cartilage bovine scaffold.Methods: This experimental laboratory research used cartilage bovine scaffold which was decellularized using physical, chemical and enzymatic techniques with different time and concentration variations, then collagen type II measurement was performed by immunohistochemical method and evaluation of porosity size with electron microscopy.Results: In the preparations with the highest concentration and the longest immersion time of 5% SDS for 72 hours, the decellularization process occurs perfectly. The collagen type II content was found to have significantly higher concentrations than the controls and other preparations, and have interconnected porosities in large size.Conclusion: High concentrations of collagen type II and large diameter of pores after decellularization process in cartilage bovine scaffold confirm that this scaffold still has optimum biochemical cues that can be a good mechanical framework and microenvironment stem cell in effort to repair the cartilage defects. This result was expected to become a standard for the decellularization making of cartilage bovine scaffold.
Comparison of 3-weekly cisplatin versus 3-weekly carboplatin in patients with locally advanced nasopharyngeal carcinoma receiving concurrent chemoradiotherapy: A multicentre analysis
Authors: Dechaphunkul Arunee , Danchaivijitr Pongwut , Jiratrachu Rungarun , Dechaphunkul Tanadech , Sookthon Chatsuda , Jiarpinitnun Chuleeporn , Paoin Chudchanok , Setakornnukul Jiraporn , Suktitipat Bhoom , Pattaranutaporn Poompis , Geater Sarayuth Lucien , Ngaimphaiboon Nuttapong 
Published: February 2020

Abstract
Background Although concurrent chemoradiotherapy (CCRT) with high-dose cisplatin remains a standard of care for patients with locally advanced nasopharyngeal carcinoma (LA-NPC), carboplatin has alternatively been used, particularly for cisplatin-ineligible patients. However, the comparable efficacy of these two regimens remains unclear. The present study aimed to evaluate the efficacy and tolerability of 3-weekly carboplatin and 3-weekly cisplatin therapies. Methods From May 2005 to November 2014, we identified patients with LA-NPC treated with CCRT from a Thai multicentre head and neck cancer database. The patients were administered a chemotherapy (CT) regimen of either cisplatin (75–100 mg/m 2 ) or carboplatin (AUC-5 or 6). Patient tolerability and survival were analysed and compared between regimens. Results Overall, 780 patients with LA-NPC were identified. Of these, the 76 patients (9.7%) treated with carboplatin showed significantly more comorbidity and lower baseline creatinine clearance than those treated with cisplatin. Compared with the cisplatin group, a higher number of patients in the carboplatin group completed three planned cycles of CT during CCRT (88.2% vs 52.0%; p <0.001) and three planned cycles of adjuvant CT (92.1% vs 78.7%; p =0.004). Furthermore, 28% of patients in the cisplatin group, as opposed to only 2.8% in the carboplatin group, required a dose reduction of CT in the subsequent cycle due to toxicities (p <0.001). At the time of analysis, the 5-year disease-free survival rate was 59.0% and 59.0% (p =0.935) and the 5-year overall survival rate was 64.0% and 66.0% (p =0.530), in the cisplatin and carboplatin groups, respectively. Conclusions Carboplatin provided comparable efficacy to that of cisplatin but with better tolerability and could be considered as an alternative regimen, particularly in cisplatin-ineligible patients with LA-NPC. These findings still warrant a randomised phase III study to compare these two CT regimens.
Motorcycle-related trauma: effects of age and site of injuries on mortality. A single-center, retrospective study.
Authors: Granieri Stefano , Reitano Elisa , Bindi Francesca , Renzi Federica , Sammartano Fabrizio , Cimbanassi Stefania , Gupta Shailvi , Chiara Osvaldo 
Published: February 2020

Abstract
Background

Motorcyclists are often victims of road traffic incidents. Though elderly patients seem to have worse survival outcomes and sustain more severe injuries than younger patients, concordance in the literature for this does not exist. The aim of the study is to evaluate the impact of age and injury severity on the mortality of patients undergoing motorcycle trauma.

Methods

Data of 1725 patients consecutively admitted to our Trauma Center were selected from 2002 to 2016 and retrospectively analyzed. The sample was divided into three age groups: ≤ 17 years, 18-54 years and ≥ 55 years. Mortality rates were analyzed for the overall population and patients with Injury Severity Score (ISS) ≥ 25. Differences in survival among age groups were evaluated with Log-Rank test and multivariate logistic regression models were created to identify independent predictors of mortality.

Results

A lower survival rate was detected in patients older than 55 years (83,6% vs 94,7%; p = 0.049) and in those sustaining critical injuries (ISS ≥ 25, 61% vs 83% p = 0.021). Age ( p =0,027; OR: 1,03), ISS ( p <0,001; OR: 1,09), Revised Trauma Score (RTS) ( p <0,001; OR: 0,47) resulted independent predictors of death. Multivariate analysis identified head ( p <0,001; OR: 2,04), chest ( p <0,001; OR: 1,54), abdominal ( p <0,001; OR: 1,37) and pelvic ( p =0,014; OR: 1,26) injuries as independent risk factors related to mortality as well. Compared to the theoretical probability of survival, patients of all age groups showed a survival advantage when managed at a level I Trauma Center.

Conclusions

We detected anatomical injury distributions and mortality rates among three age groups. Patients aging more than 55 years had an increased risk of death, with a prevalence of severe chest injuries, while younger patients sustained more severe head trauma. Age represented an independent predictor of death. Management of these patients at a Level I Trauma Center may lead to improved outcomes.
Effects of balance training on functional outcomes after total knee arthroplasty: a systematic review and meta-analysis of randomized controlled studies
Authors: Chen Wang , Hu Zheng-Hao , Sun Jian-Ning , Zhang Ye , Zhang Yu , Chen Xiang-yang , Feng Shuo 
Published: February 2020

Abstract
Background: Many previous studies have compared the effects of preoperative balance training and non-balance training on daily performance and knee functional outcomes after surgery, however, whether preoperative balance training is more beneficial to patients is still a big debate. Comparing the postoperative joint and daily function of balance training and non-balance training is the main purpose of our study.Methods: Cochrane library, Pubmed and Web of Science databases searched by us, and searched again before submitting our submission. This mete-analysis included 22 studies that directly compared postoperative performance and functional outcomes after training group (TG) and control group (CG). We used the software endnote X9 for data selection, and the software Review Manager 5.3 for data analysis to make funnel plots and forest plots.Results: The pooled data indicated that balance training significantly improved 2/6 MWT (2/6-min walk test) (weighted mean difference (WMD) = 25.17, 95% confidence interval (CI) 12.88 to 37.46, P < 0.0001), gait speed (WMD = 0.15, 95% CI 0.10 to 0.19, P < 0.00001), TUG (timed up and go) (WMD = 1.02, 95% CI 0.75 to 1.29, P < 0.00001), BBS (berg balance scale) (WMD = 1.79, 95% CI 0.50 to 3.08, P = 0.006), FHR (ratio of functional reach distance to body height) (WMD = 9.34, 95% CI 6.69 to 11.98, P < 0.00001), TCS (WMD = 1.20, 95% CI 0.86 to 1.53, P < 0.00001), early stage vitality (WMD = 14.41, 95% CI 13.53 to 15.30, P < 0.00001) and KOOS (Knee Injury and Osteoarthritis Score) sysptoms (WMD = 6.34, 95% CI 2.07 to 10.60, P = 0.004), middle stage function (WMD = 5.85, 95% CI 0.13 to 11.56, P = 0.04).Conclusion: Preoperative balance training improved partial postoperative daily performance and knee functional outcomes in patients with total knee arthroplasty.
Sediment microbial communities contribute to shrimp intestine microbiota in cultural pond ecosystems
Authors: Huang Zhijian , Hou Dongwei , Zhou Renjun , Zeng Shenzheng , Xing Chengguang , Wei Dongdong , Deng Xisha , Yu Lingfei , Wang Hao , Deng Zhixuan , Weng Shaoping , Ning Daliang , Xiao Chuanle , Yan Qingyun , Zhou Jizhong , He Zhili , He Jianguo 
Published: February 2020

Abstract
Background

Microorganisms in a shrimp cultural pond ecosystem (SCPE) play important roles for animal and environmental health. While the microbial diversity and assembly mechanism, and how communities of multiple habitats contribute to the SCPE metacommunity are poorly understood. Here, we analyzed phylogenetic structure of 792 samples of three habitats (water, shrimp intestine and sediment) from 88 shrimp cultural ponds across six regional sites in China.

Results

We show that the microbial communities of three habitats in the SCPE contained 15,197 operational taxonomic units (OTUs), and the microbial community of sediment had the highest OTUs (14,857). Despite the high microbial diversity, the SCPE has small core taxa (13 OTUs) among three habitats, and 30, 15, 53 core OTUs in water, shrimp intestine, and sediment, respectively. Especially, some opportunistic pathogens or functional microbes were core taxa in shrimp intestine or water and sediment. Of the SCPE metacommunity, the microbial diversity of the SCPE was mainly contributed by‾αLocalCommunities (66.0%), and followed by βInterHabitats (29.0%). The sediment microbial communities had a larger contribution (56.8%) to shrimp intestine microbiota compared to water communities (18.1%), which was also corroborated by the Sloan neutral community model analysis. Further, microbial assembly of three habitats appeared to be largely driven by stochastic processes (over 78%) in the SCPE.

Conclusions

Our results demonstrate that those core microbial taxa of three habitats were distinct with each other, that sediment communities mainly contributed to shrimp intestine communities, and that the microbial assembly was largely driven by stochastic processes. Our study enhances the mechanistic understanding of the microbial diversity and assembly in the SCPE for further analyzing metacommunities, and has important implications for microbial ecology and shrimp culture strategies.
An innovate strategy to treat large metaphyseal segmental femoral bone defect using customized design and 3D printed micro-porous prosthesis: a prospective clinical study
Authors: Hou Guojin , Liu Bingchuan , Tian Yun , Liu Zhongjun , Zhou Fang , Ji Hongquan , Zhang Zhishan , Guo Yan , Lv Yang , Yang Zhongwei 
Published: February 2020

Abstract
Background Large segmental bone defect at the metaphyseal area is still difficult to treat, nowadays, there is a tremendous level of interest in uses of 3D printing technology in orthopaedic surgery. This study was introduced to prospectively confirm the safety and effectiveness of 3D printed micro-porous prosthesis in clinical bone defect reconstruction application. Methods Patients with segmental irregular-shaped bone defect of the femur were recruited from 2017.12 to 2018.11. The first stage of the treatment involves radical debridement of all infected or non-viable bone and interposed fibrous tissue, and temporary fixation. Once the culture and biopsy results were negative, the PMMA spacer should remain in the defect approximately 6-8 weeks. This period is for the membrane formation, virtual surgery (computed tomography (CT) scan of the lesion area and the contra-lateral parts of the femur, and then design of the implant). The second stage involves reconstruction the defects with the 3D printed micro-porous prosthesis combined with intra-medullar nailwithout bone graft.Routine clinical follow-up and radiographic evaluation were done to assess bone incorporation and complications of internal fixation. The weight-bearing time and the joint function were recorded. Result 5 consecutive patients were included in the study. They were followed up for an average of 16.4 months. The average length of bone defect and the distal residual bone was 12 cm and 6.5 cm. The average time of partial weight-bearing and full weight-bearing was 12.7 days and 2.6 months. X-ray demonstrated good osseous integration of the implant/bone interface. No complications occurred such as implant loosening, subsidence, loss of correctionand infection. At the last follow-up, Harris score of hip joint was excellent in 2 cases, good in 2 cases, fair in 1 case; HSS score of knee joint was good in 4 cases, middle in 1 case. Conclusion Meticulous customized design 3D printed micro-porous prosthesis combined with intramedullary fixation may be a cost-effective and an alternative strategy to treat metaphyseal segmental irregular-shaped femoral bone defect without bone graft, especially for cases with massive juxta-articular bone loss.
Hepatic arterial infusion chemotherapy versus Sorafenib for advanced hepatocellular carcinoma with portal vein tumor thrombus: a systematic review and meta-analysis
Authors: ouyang guoqing , Pan Guangdong , Liu Qiang , Wu Yongrong , Tan shengqiang , Lu Wuhang , Li Shuai , xu honglai 
Published: February 2020

Abstract
Background : Hepatocellular carcinoma (HCC) is ranked as the sixth most common solid cancer and the third leading cause of cancer-related death in the world. Sorafenib is the first line systematic treatment for patients with advanced HCC. Hepatic arterial infusion chemotherapy（HAIC）has been proved to be an effective treatment for advanced HCC. Here, we conducted a meta-analysis to compared the efficacy of HAIC versus sorafenib of advanced HCC patients with PVTT. Methods : The databases of MEDLINE (PubMed), Cochrane Library, EMBASE, and Web of Science were systematically searched for retrieving the relevant publications before 31 July 2019. The endpoint included overall survival (OS), time to progression (TTP), partial response rate (PRR), complete response rate (CRR), objective response rate (ORR), stable disease rate (SDR). Results : A total of three studies involving 214 advanced HCC patients with PVTT enrolled in this meta-analysis. HAIC significantly improved TTP (hazard ratio (HR) = 0.56, 95% CI: 0.39-0.82; P = 0.003), PRR (odds ratio (OR) = 3.31, 95% CI: 1.46-7.50; P = 0.004), ORR (OR = 3.78, 95% CI: 1.68-8.50; P = 0.001) compared to sorafenib. However, no significant difference was found in OS (HR=0.77, 95%CI: 0.56-1.06, p=0.11), CRR (OR = 2.54, 95% CI: 0.39-16.47; P = 0.33), SDR (OR = 1.48, 95% CI: 0.43-5.08; P = 0.001). Conclusions : This meta-analysis suggested that HAIC provides better TTP, PRR, ORR than sorafenib for patients of advanced HCC with PVTT. Therefore, we recommend HAIC as a potential therapy for advanced HCC patients with PVTT. However, owing to the above limitations, more high-quality studies are warranted to evaluate this finding.
Quercetin promotes long noncoding RNA lncSHGL expression via estrogen receptor α to suppress type 2 diabetes mellitus in mice
Authors: Fan Hanlu , Li Haiwen , Liu Huijiao , Li Peng , Jia Xiaomeng , Guo Peilan , Yu Qingquan , Li Xiru , Guo Yangdong , Li Xiangdong 
Published: February 2020

Abstract
Background

Type 2 diabetes mellitus (T2DM) is the most common type of metabolic disorder involving multiple organ systems. Grape has been reported to improve the symptoms of T2DM, the precise mechanism of its action is unclear. Our study was aimed to determine the effect and mechanism of grape pomace extract in T2DM mice induced by high fat diet (HFD).

Materials and methods

Ultra-performance liquid chromatography and quadrupole time-of-flight mass spectrometry were used to identify the main active compounds in grape pomace extract to improve T2DM. C57BLK/6J mice induced by HFD supplemented with or without quercetin were used to show the effects of quercetin improving T2DM. By online database research, bioinformatics analysis and molecular biology experiments, Estrogen receptor alpha (ERα)-lncSHGL (lncRNA suppressor of hepatic gluconeogenesis and lipogenesis) pathway was identify as the target for quercetin.

Results

Quercetin was identified as one of the most active compounds in grape pomace extract to improve T2DM. Quercetin could inhibit HFD-induced T2DM in mice by activing ERα. LncSHGL was identified as the downstream of ERα and inhibited HFD-induced T2DM.

Conclusions

Quercetin could be beneficial for T2DM by promoting lncSHGL transcription and activating the lncSHGL pathway, and may be used as a drug component to treat T2DM.
Epidemic analysis of COVID-19 in China after Wuhan was restricted
Authors: yu anfeng , wang zhiwei , ren wei , wu zhiyong , hu zhipeng , li luocheng , ruan yongle , hu rui , shi feng 
Published: February 2020

Abstract
BackgroundIn December 2019, Coronavirus Disease 2019(COVID-19) caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infection appeared in Wuhan, Hubei Province, China. The disease is highly infectious. Wuhan, Hubei Province decided restrict personnel movement on January 23.We analyze relevant data to show the situation of the COVID-19 epidemic in China.MethodsThe data was classified according to Hubei group, non-Hubei group, Hong Kong, Macao and Taiwan group, and Chinese Mainland group, and analyze the current situation and trend of the epidemic.ResultsThere was an explosive growth in the early stage of the epidemic. The epidemic situation began to improve in about two weeks after Wuhan was restricted,and the situation in non-Hubei was significantly better than that in Hubei.ConclusionThe blockade of Wuhan was a correct decision, cut off the outflow of tinfection sources, and the epidemic situation in all places turned around after the incubation period.
Efficacy and safety of different mechanical ventilation strategies for patients with acute respiratory distress syndrome: Systematic review and network meta-analysis
Authors: Huang Wenqi , Wang Pengfei , Jia Fang , Huang Bin , Chen Xiaodong , Du Hu , Sun Hang , Liu Qi , Zhang An 
Published: February 2020

Abstract
Background: Acute respiratory distress syndrome (ARDS) leads to life-threatening acute hypoxemic respiratory failure in clinic and usually requires mechanical ventilation. Low tidal volume ventilation is now universally accepted as management treatment for this condition. However, recruitment maneuvers (RM) and positive end-expiratory pressure (PEEP) remain controversial. Therefore, we performed a network meta-analysis to identify the RM and PEEP levels of patients with ARDS.Methods: We searched PubMed, OVID, Embase, and the Cochrane Central Register of Controlled Trials (Central) databases. The primary outcome was death in the 28th day, and the secondary outcomes included death in hospital, ventilator-free days, and barotrauma. Data for study characteristics, methods, and outcomes were extracted. The relative effect sizes were estimated by risk ratios (RRs) for binary variables and standard mean difference (SMD) for continuous variables. Relative ranking of the interventions was conducted using surface under the cumulative ranking. Multiple intervention comparisons based on the Bayesian and frequentist frameworks were performed to integrate the efficacy of all included strategies.Results: Thirty randomized controlled trials comprising 4410 patients were included in the network meta-analysis. None of the ventilation strategies was significantly superior over others for all outcomes. According to the relative rank probabilities, low PEEP showed the lowest probability of harming death in the 28th day, whereas RM+low PEEP showed the highest probability of benefitting death in hospital and ventilator-free days. Low PEEP showed the highest probability of benefitting barotrauma. The overall quality of the evidence per grade was moderate to low.Conclusions: No ventilation strategy is significantly superior over others. RM+low PEEP has the highest probability of benefitting survival. The evidence has low overall quality and should be further studied.
Vitamin D Receptor Polymorphisms in the Turkish Population are not associated with Multiple Sclerosis
Authors: Hoscan AY , Bulan Batuhan , Keskin Sena Nur , Cavus Ayse , Culcu Elif Asena , Isik Nihal , List Edward O. , Arman Ahmet 
Published: February 2020

Abstract
Background Multiple sclerosis (MS) is an inflammatory disease characterized by demyelination and axonal degeneration affecting the central nervous system (CNS). Unfortunately, very little is known about the etiology of this disease. Among the genetic factors suggested to be associated with this disease are polymorphisms to the vitamin D receptor (VDR). However, there is disagreement in the literature on this topic. Therefore, we tested the hypothesis that polymorphisms in the vitamin D receptor (VDR) are also associated with MS. Aim The aim of the study was to investigate the relationship of MS disease with VDR Fok-I, Bsm-I and Taq-I polymorphisms in Turkish population. Method This study contains 175 MS patients and 183 healthy controls. Blood samples were taken from each subject to isolate genomic DNA by salting out method. VDR gene Fok-I, Bsm-I and Taq-I polymorphism regions for each patient and control were amplified by polymerase chain reaction (PCR). The PCR products were digested, and the genotypes were determined based on size of digested PCR products. Results No correlation was found between MS disease and VDR Fok-I, Bsm-I and Taq-I polymorphisms for genotype distribution (Pearson test, p>0,05 ) or allele frequency (Pearson test, p>0,05 ). No meaningful association was found between subtypes of MS for genotypes distribution and allele frequencies of Fok-I, Bsm-I and Taq-I polymorphisms (Pearson test, p>0,05 ). Conclusion There is no association between VDR gene polymorphisms (Fok-I, Bsm-I and Taq-I) and MS disease in Turkish population.
Mass spectra analysis of quinoline alkaloids detected in Sauuda fruticose leads to novel biosynthesis pathway of quinoline alkaloids
Authors: Al-ghazzawi Adel M. , Alshehri Mohammed A , Deeb Ahmad A , zarga Musa H Abu , Alalmie Ali Y 
Published: February 2020

Abstract
Alkaloids reported to have a wide range of biological activity moreover, the biological effects of alkaloids depends on the type of the alkaloids and since the phytochemical screening studies on Suaeda fruticosa indicates the presence of alkaloids but they did not determine the types of the alkaloids present on this plant. Consequently, the LC-MS/MS analysis of the alkaloidal part of Suaeda fruticosa leave extarct reveals the presence of six quinoline alkaloids beside that mass spectra analysis of the present quinoline alkaloids leads to suggest a new biosynthetic pathway of quinoline alkaloids.