bluuebunny/crossref_metadata_2025_split huggingface dataset:
The application of team-based learning in emotional intelligence training for medical students: A pre–post-test study
Authors: Yang Wenjiao , Chen Yu , Gao YuanMin , Wang Jing , Fang Fan , Xiao JuLan 
Published: April 2020

Abstract
Background

Emotional intelligence represents a person’s interpersonal and communication competencies, and influences medical students and their clinical careers. The aim is to evaluate the impact of team-based learning in an emotional intelligence training intervention among Chinese medical students.

Methods

This is a quasi-experimental, one-group pre–post-test assessment. A convenience sample of medical university students took an elective course in emotional management recruited for this study. An emotional management course was designed to provide college students with basic knowledge about emotional regulation and to provide opportunities for emotional management practice. The course composed of traditional face-to-face education and the new style of teamwork. They completed the educational modules using their personal computers or cell phones. Using the Emotional Intelligence Scale, Caring Ability Inventory, and a course evaluation questionnaire, two research assistants collected data before and after delivery of the intervention. Descriptive statistics were calculated for sociodemographic data. Categorical data were described as frequencies, and continuous data were expressed as means. Differences in respondent characteristics between the pre- and post-intervention assessments were investigated using the chi-squared test. The paired-samples t test was used to investigate differences between pre- and post-intervention. Ninety-eight students completed the pre-intervention questionnaire and 82 students completed the post-intervention questionnaire.

Results

The intervention improved students’ emotional intelligence and caring ability, as indicated by increased scores in perceiving and expressing emotions (t = 7.045, P < 0.01), regulating emotions (t = 6.094, P < 0.001), knowing (t = 4.268, P < 0.001), and courage (t = 4.842, P < 0.001). Students' average course evaluation ratings were >4 points (The total score is 5.).

Conclusions

This intervention has the potential to influence medical students’ emotional intelligence and caring behavior.
Histone H4 Aggravates Inflammatory Injury Mainly Through TLR4 in Chlorine Gas Induced Acute Respiratory Distress Syndrome
Authors: Zhang Yanlin , Zhao Jian , Guan Li , Mao Lijun , Li Shuqiang , Zhao Jinyuan 
Published: April 2020

Abstract
Background: Chlorine gas (Cl2) exposure remains a public health concern in household, occupational, and transportation accidents around the world. The death rate associated with acute respiratory distress syndrome (ARDS) caused by high concentrations of Cl2 is very high, mainly because the pathogenesis of ARDS is still not very clear. Histone H4 has been identified as an important endogenous pro-inflammatory molecule. The present work aimed to study the pathogenic role of histone H4 in Cl2 induced ARDS. Methods: ARDS was induced by Cl2 exposure in male C57BL/6 mice. Circulating histone H4, blood gas, pulmonary edema, endothelial activation, and neutrophil infiltration were measured during acute lung injury. Histone H4 or anti-H4 antibody was administered through tail vein 1h prior to Cl2 exposure to study the pathogenic role of histone H4. The Toll-like receptor 2 knock-out (Tlr2-KO), Tlr4-KO mice and the blocking antibody against TLR1, TLR2, TLR4 or TLR6 were used to explore the mechanism involved in histone H4 mediated injury. Results: Cl2 exposure induced a concentration dependent acute lung injury. The levels of circulating histone H4 were positively correlated with the concentrations of Cl2. Pretreatment with intravenous histone H4 further aggravated lethality rate, blood gas, endothelial activation, and neutrophil infiltration while the anti-H4 antibody showed protective effects. The deﬁciency of Tlr4 improved lethality rate, blood gas, and pulmonary edema; prevented endothelial and neutrophil activation caused by Cl2 exposure. More importantly, the gene deletion of Tlr4 diminished greatly the effect of histone H4 or anti-H4 antibody observed in the wild-type (WT) mice. The impact of Tlr2 on inflammatory injury was not significant. The role of TLRs was also validated by endothelial activation mediated by histone H4 in vitro. Conclusions: Circulating histone H4 played a pro-inflammatory role in ARDS caused by Cl2. TLR4 was closely involved in histone H4 mediated inflammatory injury. The intervention targeted at histone H4 is potentially protective.
Effects on applying Micro-Film Case-Based Learning model in pediatrics education
Authors: Pan Yuan , Chen Xiuqi , Wei Qiuwen , Zhao Jinmin , Chen Xun 
Published: April 2020

Abstract
Background: By establishing an innovative teaching model, teachers and students can optimize the education outcome. Lecture-Based Learning (LBL) model has long been widely used as main teaching method in medical course in Chinese culture with a positive result of grades, however, negative of practice. Case-Based Learning (CBL) model modified it by focusing on clinical case study, yet with its limitations. The aim of our study is to discuss the effect of applying a comprehensive renewal CBL model: Micro-Film Case Based Learning (MF+CBL) Model, in pediatrics theoretical course in senior year students under Chinese culture.Methods: Experimental research was conducted by parallel group control study. The total sample was 104 senior year students (Chinese) majoring in clinical medicine from Guangxi Medical University. The experimental group was intervened by MF+CBL model, while the control group by LBL model. Both process and result were assessed after 8-week courses, by questionnaires of Student Self-Assessment, Satisfaction Survey and Final examination.Results: The outcome of experimental group generally surpassed control group in Student Self-Assessment, Satisfaction Survey, and Final examination. The open question at the end of the questionnaire yet unfolded a fact that a small number of students were not used to the MF+CBL model due to its time and energy consuming feature.Conclusions: MF+CBL model was an innovative teaching method, compared with LBL, with a better contribution of comprehensive quality development，which offered an alternative model to optimize the capacity of future pediatrics doctors.
Optimal initial cell density that yields the highest number of primary synovial mesenchymal stem cells in a clinical setting
Authors: Horiuchi Kiyotaka , Mizuno Mitsuru , Katano Hisako , Endo Kentaro , Ozeki Nobutale , Tsuji Kunikazu , Koga Hideyuki , Sekiya Ichiro 
Published: April 2020

Abstract
Background Synovial mesenchymal stem cell (MSC) transplantation improved clinical outcomes in knees with cartilage defects and after repairs of degenerative torn menisci. For these, nucleated cells were cultured with 10% autologous serum for 14 days. Importantly, nucleated cell number and serum volume practically varied by patient, and initial cell density and dish number affected yield/donor. Our purpose was to examine the effect of plating density within range in clinical studies on fold increase and to estimate the MSC yield/donor, which are affected by the nucleated cell number and serum volume. Methods We obtained the data of the autologous serum volume, nucleated cell number, and initial plating cell density from 10 patients who participated in our previous clinical studies. Experimentally, synovial nucleated cells harvested from three patients with osteoarthritis during total knee arthroplasty were cultured with 10% mixed allogenic human serum for 14 days to examine the effect of initial cell density on fold increase. We used both our previous clinical data and our current experimental data to predict the yields of synovial MSCs in nine cases within the range of clinical studies. Results In the clinical studies, collected serum ranged between 87 and 186 ml, harvested nucleated cells ranged between 6.2 and 23 million, and the top five total MSC yields were obtained from donors when the initial plating cell density ranged from 919 to 1905 cells/cm 2 . Experimentally, fold increase significantly decreased between 700 and 1,900 cells/cm 2 . In cases where the cell number was low and the serum volume was high, the estimated yield/donor decreased along with the initial density, while in cases where the cell number was high and the serum volume was low, the estimated yield/donor increased along with the initial density. In cases both where some nucleated cells were discarded at low density and no cells were discarded at high density, the maximum yield/donor were obtained when the initial density was the lowest density that does not discard cells. Conclusions There was an optimal initial density that yields the highest number of primary MSCs in a clinical setting when cell numbers and human serum volumes are limited.
Ovarian reserve after three-step laparoscopic surgery for endometriomas utilizing dienogest: a non-randomized prospective pilot study
Authors: Kitajima Michio , Matsumoto Kanako , Murakami Naoko , Harada Ayumi , Kitajima Yuriko , Masuzaki Hideaki , Miura Kiyonori 
Published: April 2020

Abstract
BackgroundSurgery for endometriomas may cause detrimental effects on ovarian reserve. In this study, we evaluated the efficacy of three-step laparoscopic surgery for endometriomas utilizing novel progestin, dienogest in terms of post -surgical ovarian reserve.MethodsTwelve women received first look laparoscopy (FLL) with fenestration and drainage. Immediately after the surgery, they took oral dienogest 2mg for three months, then they received second look laparoscopy (SLL) with cystectomy. We compared the effects on ovarian reserve by serum AMH levels between women had three step management with dienogst and another twelve women had　conventional one-step surgery without medications. In women had three-step surgery with dienogest, the changes in concentration of proinflammatory cytokines and chemokines (interleukin (IL)-1β, IL-6, IL-8, tumor necrosis factor (TNF)-α, monocyte chemotactic protein (MCP)-1) in peritoneal fluids were evaluated.ResultsSerum AMH levels were significantly decreased after three months of dienogest following FLL. AMH levels were also significantly decreased 3-6 month both after SLL and after one-step surgery, however, recovery of serum AMH levels at 9-12 months after surgery were evident in women had three-step surgery comparing to those of women had one-step surgery. Proinflammatory cytokines and chemokines in peritoneal fluids were down regulated at the time of SLL comparing to those of FLL.ConclusionsThree-step surgery with dienogest may be a beneficial approach to protect ovarian reserve. Dienogest may exert its effects in part by lowering proinflammatory cytokines and chemokines.
Growth patterns and associated risk factors of congenital malformations in twins
Authors: Piro Ettore , Schierz Ingrid Anne Mandy , Serra Gregorio , Puccio Giuseppe , Giuffrè Mario , Corsello Giovanni 
Published: April 2020

Abstract
Background: The rate of twinning continues to increase due to the combined effect of a rise in parental age and increased use of assisted reproductive technology. The risk of congenital anomalies in twins is higher than in singletons, but it is less well reported in relation to growth patterns. We focused to the auxological outcome of twin pregnancies when one or both of twins are affected by one or more malformations. Methods: We conducted a retrospective observational study reviewing the clinical charts of twins admitted in the period between January 2003 and December 2018 at the University Hospital of Palermo. The associations between malformations and anthropometric variables at birth were analyzed by comparison within each twin pair and regarding each variable as ordered difference between the two twins. Results: We studied data of 488 neonates (52% females) from 244 pregnancies. The rate of major congenital anomalies was 11%, affecting significantly the smaller twin (p = .00018; Odds ratio 3.21; 95% CI 1.65 6.59). Malformation class distribution was as following: genitourinary (24%), gastrointestinal (20%), cardiovascular (18.5%), musculoskeletal (11%), central nervous system (9%), syndromic (9%), ocular (5.5%) and diaphragmatic hernia (2%). The most predictive value, the Birthweight (BW) difference mean ratio in malformed versus not malformed neonates (-0.31 vs 0.02; p= .0016) was distributed equally lower than zero in all malformed twins, except for those with congenital heart defects ( p= .0000083). Microcephaly (head circumference <2 standard deviation, SD) was present in 3% of symmetrically smaller twin, and severe microcephaly (<3 SD) was present in 0.6%. We found that an intertwin BW discordance of 18% or greater identified 50% of neonates with microcephaly, but only 11% of malformed twins. Conclusions: In case of one twin with a BW <10 th centile, a concomitant intertwin BW discordance ≥18% could reveal an increased risk for microcephaly but not for malformation. Lower values of BW, Ponderal index, Body mass index but above all negative value of BW difference mean ratio are associated with malformations in twin pairs.
A rare symptom of ofactory dysfunction in coronavirus disease 2019: report of three cases
Authors: ChenChen  , Chen Moxian , Cheng Cong , Chi Yun , Hu Zhiliang , Liu Yuan , Huang Shan , Lv Yanling , Liang Chongfeng , Jiao Damin , Yi Yongxiang , Zhang Xia , Sun Wenkui , Wei Hongxia 
Published: April 2020

Abstract
Three patients of coronavirus disease (COVID-19) showed the symptoms of olfactory dysfunction. Clinical characteristics and treatment were retrospective analyzed. Olfactory disorders are uncommon symptoms of COVID-19. Early diagnosis and intervention are key to the recovery of olfactory disorders. Particular attention should be devoted to rare symptoms of COVID-19.
The “Talk Test” is a practical tool to determine aerobic training zones in overweight and obese patients.
Authors: Orizola-Cáceres Ignacio , Cerda-Kohler Hugo , Burgos-Jara Carlos , Meneses-Valdes Roberto , Gutierrez-Pino Rafael , Sepulveda Carlos 
Published: April 2020

Abstract
Background: to validate the traditional talk test (TTT) and an alternative talk test (ATT; using a visual analog scale) in overweight/obese (OW-OB) patients and to establish its accuracy in determining the aerobic training zones.Methods: We recruited 19 subjects aged 34.9 ± 6.7 years, diagnosed with overweight/obesity (BMI 31.8 ± 5.7). Every subject underwent incremental cycloergometric tests for maximal oxygen consumption, and TT in a randomized order. At the end of each stage during the TT each subject read out loud a 40 words text and then had to identify the comfort to talk in two modalities: TTT which consisted in answering “Yes”, “I don’t know” or “No” to the question Was talking comfortable?, or ATT through a 1 to 10 numeric perception scale (visual scale analog: VAS). The magnitude of differences was interpreted in comparison to the smallest worthwhile change (SWC) and was used to determine agreement.Results: Agreement between the power output at the VAS 2-3 of ATT and the power output at the ventilatory threshold 1 (very likely equivalent; mean difference -1.3 W, 90 % CL (-8.2; 5.6), % chances for higher/similar/lower values of 0.7/99.1/0.2 %). Also, there was an agreement between the power output at the VAS 6-7 of ATT and the power output at the ventilatory threshold 2 (very likely equivalent; mean difference 11.1 W, 90 % CL (2.8; 19.2), % chances for higher/similar/lower values of 0.0/97.6/2.4 %). Conclusions: ATT is a tool to determine exercise intensity and to establish aerobic training zones for exercise prescription in OW-OB patients.
Clubfoot treatment with Ponseti method – Parental distress during plaster casting
Authors: Walter Christian , Sachsenmaier Saskia , Wünschel Markus , Teufel Martin , Götze Marco 
Published: April 2020

Abstract
Background: Clubfoot is one of the most prevalent musculoskeletal congenital defects. Gold standard treatment of idiopathic clubfoot is the conservative Ponseti method, including the reduction of deformity with weekly serial plaster casting and percutaneous Achilles tenotomy. It is well known that parents of children with severe and chronic illnesses are mentally stressed, but in recent studies regarding clubfoot treatment, parents were only asked about their satisfaction with the treatment. Largely unknown is parental distress before and during plaster casting in clubfoot.Therefore, we want to determinate first, how pronounced the parents' worries are before treatment and if they decrease during the therapy. Second, we hypothesized that parents faced with an extreme deformity (high Pirani-Score), reveal more distress, than parents whose children have a less pronounced deformity (low Pirani-Score). Therefore, we wanted to investigate weather the Pirani score correlates with the parents' mental resilience in relation to the therapy of the child as a global distress parameter.Methods: To answer this question, we developed a questionnaire with the following emphases: Physical capacity, mental resilience, motion score, parents score and child score with point scores 1 (not affected) to 6 (high affected). Subsequently, we interviewed 20 parents whose children were treated with clubfeet and determined the Pirani score of the infants at the beginning (T0) and at the end (TE) of the treatment with plaster casting.Results: High values were obtained in child score (Mean (M) = 3.11), motion score (M = 2.63) and mental resilience (M = 2.25) and. During treatment, mental resilience improved (p = 0.015) significantly. The Spearmann correlation coefficient between Pirani-Score (T0) and mental resilience (T0) is 0.21, so the initial hypothesis had to be rejected. Conclusion: The issues of the children are in the focus of parental worries concerning clubfoot treatment, especially the assumed future motion and the assumed ability to play with other children. Particular emphasis should be placed on educating parents about the excellent long-term results in the function of the treated feet especially as this topic shows the greatest parental distress.
Building a non-homogeneous environment for high effective cooling in the face of deep mines
Authors: Li Xian , Fu Houli 
Published: April 2020

Abstract
Heat exhaustion of mining environments can cause a great threat to human health. The existing cooling strategies for the mine face aim to cool the whole face. However, the area of effective air-cooling space for the face is small, and a great amount of energy for cooling is ineffective. Effective air-cooling space is a space occupied by the workers in the face. This study proposed to build a non-homogeneous environment for high effective cooling in the face. An inlet air cooler was laid out in the intake airway to cool the whole face to some extent, and the tracking air cooler was designed to track the worker who constantly moved to improve the thermal environment. The cooling load and air distribution for this cooling strategy were investigated. In addition, the airflow in the face was numerically solved to estimate the cooling effect. The results revealed that an average energy saving of approximately 35% could be achieved. The thermal environment of the effective air-cooling space within at least 10 m was significantly improved. This cooling strategy should be taken into account in mine cooling.
Absence of High Penetrance Genes Mutations in Familial Breast Cancer in Mizo-Mongloid Population, Northeast India
Authors: Zodinpuii Doris , Zothankima Bawitlung , Pautu Jeremy Lalrinsanga , Lallawmzuali Doris , Varma Ashok Kumar , Varma Ashok kumar , Muthukumaran Rajendra Bose , Kumar Nachimuthu Senthil 
Published: April 2020

Abstract
Background: Breast cancer is the most prevalent cancer and leading cause of death among women globally. The present study focuses on screening germline mutations of breast cancer susceptibility genes among the unexplored Mizo breast cancers of culturally and historically homogenous population, living a unique life style habits in terms of diet and tobacco usage. Methods Mutation screening was performed using Sanger sequencing in complete coding region of BRCA1 and frequently mutated exons of TP53, PTEN, CDH1, CHEK2 and XRCC2. Several online mutation prediction tools and databases were used to check the pathogenicity of the polymorphisms observed. Results: We observed eight polymorphisms in total, in which, one variants p.P1544P in BRCA1 gene was found to be novel. No variants were found to have a potential impact on protein since all the polymorphisms are of silent substitutions. No genetic alteration was observed in the studied exons of each of TP53, PTEN, CDH1, CHEK2 and XRCC2. Conclusion: To our knowledge, the present study focusing on familial breast cancer is the first time to analyzed the prevalence of breast cancer susceptibility gene mutations using direct sequencing in Mizo population. Even though, we do not find significant amino acid change, our results suggest the need for further evaluation of broader panel genes and a challenge to screen larger sample size to establish the contribution of these gene mutations in this population.
Quantity not quality: promoting sustainable wastewater practices in Jordan
Authors: Mohammad Alsharifa Hind , Hazimeh Wisam , Shatanawi Khaldoun , Abualhaija Mahmoud 
Published: April 2020

AbstractJordan, the second most water-scarce country in the world, is gradually increasing its reliance on treated wastewater for its water supply; more than 90% of treated wastewater is used either in agricultural or industrial activities in Jordan. However, in Jordan, all treated wastewater plants are constructed upstream of dams, the latter being used to store treated wastewater. Most dams in the country were originally constructed to collect freshwater through rainfalls. Mixing this source of freshwater with treated wastewater decreases the dams' water quality. This study examines the effects of mixing freshwater with treated wastewater in dams by comparing water samples from the outflows of selected wastewater treatment plants and different dams with historical hydrochemical data of dam water before the diversion of treated wastewater. This study finds that the quality of dam water, in which freshwater has been mixed with treated wastewater, notably decreases. Hence, this study formulates policy recommendations on how to ensure a sustainable water supply that ensures the quality necessary to different water uses, making the suggestion of a separate storage system in dams.