bluuebunny/crossref_metadata_2025_split huggingface dataset:
PRODUKSI HIDROLISAT PROTEIN ANTIOKSIDAN MELALUI HIDROLISIS ENZIMATIK PROTEIN KULIT AYAM BROILER DENGAN ENZIM PAPAIN
Authors: Puspawati N. M. , Dewi P. P. , Bogoriani N. W. , Ariati N. K. 
Published: December 2020

Penelitian ini bertujuan untuk menentukan aktivitas antioksidan hidrolisat protein yang dihasilkan dari proses hidrolisis enzimatik protein kulit ayam dengan menggunakan variasi konsentrasi enzim papain. Proses hidrolisis dilakukan dengan variasi konsentrasi enzim 1, 3,dan 5% (b/b protein), pada pH 7, suhu 50oC dan waktu hidrolisis 24 jam. Parameter yang diukur meliputi derajat hidrolisis, persen peredaman radikal DPPH (diphenylpicryl hidrazyl), dan komposisi asam amino hidrolisat protein yang paling aktif antioksidan. Hasil penelitian menunjukkan penggunaan konsentrasi enzim yang berbeda menghasikan hidrolisat protein dengan derajat hidrolisis dan persen peredaman DPPH yang berbeda. Penggunaan konsentrasi enzim papain 1, 3, dan 5% menghasilkan hidrolisat protein dengan derajat hidrolisis berturut-turut sebesar 9,3±1,8, 18,09±5,6, dan 23,15±6.33%, dan persentase hambatan radikal DPPH pada konsentrasi uji 100 ppm berturut-turut sebesar 16,75±0,07, 58,35±0,00, dan 52,99±0,07%. Hidrolisat protein yang diperoleh menggunakan enzim papain 3% (b/b protein) menunjukkan persen peredaman tertinggi dengan nilai IC50 92,98 ppm, memiliki komposisi asam amino tertiggi asam glutamat dan terendah valine.
Kata kunci: antioksidan, enzim papain, hidrolisat protein, kulit ayam
This study aimed to determine the antioxidant activity of protein hydrolyzate produced from the enzymatic hydrolysis of chicken skin protein by using variations of the enzyme papain concentration. The hydrolysis process was carried out with variations in the concentration of the enzymes 1, 3, and 5% (w/w protein base), at pH 7, temperature 50oC, and hydrolysis time 24 hours. The parameters measured including degree of hydrolysis, percentage of inhibition of DPPH radical (diphenylpicryl hydrazyl) and amino acid composition of the protein hydrolyzate with the highest antioxidant activity. The results showed that the use of different enzyme concentrations resulted in protein hydrolyzate with different degrees of hydrolysis and percentage of inhibition of DPPH. The use of papain enzymes 1, 3, and 5% produced protein hydrolyzate with the degree of hydrolysis of 9.3 ± 1.8, 18.09 ± 5.6, and 23.15 ± 6.33%, and the percentage of inhibition of DPPH radical at a concentration of 100 ppm 16.75 ± 0.07, 58.35 ± 0.00, and 52.99 ± 0.07% respectively. Protein hydrolyzate obtained using the enzyme papain 3% (w/w protein) exhibited the highest percentage of inhibition of DPPH with an IC50 value of 92.98 ppm, having the highest amino acid composition of glutamic acid and the lowest valine.
Keywords: antioxidant, enzyme papain, protein hydrolyzate, chicken skin
Strategi Pemasaran Pantai Dream Island Di Mertasari Desa Intaran Sanur Kauh Kota Denpasar
Authors: Samosir Laura Therecia , Mahagangga I Gusti Agung Oka 
Published: December 2020

Tourism in Indonesia has recently grown rapidly in one of them in the city of Bali. Bali has a very beautiful natural potential, one of the natural potentials developed is coastal tourism. Dream Island Mertasari Sanur Beach is one of the beaches that is still new but has a lot of potentials. Therefore, this study was conducted to determine the existing conditions and marketing strategies that exist on Dream Island the Mertasari Beach Sanur Kauh City of Denpasar.
The method used in this study is a qualitative descriptive method with data sources in the form of primary data and secondary data. The primary data in this study are data sourced from direct observation to the location of the study and by interviewing informants. While secondary data in this study are data obtained from documentation and literature to complete the primary data. The technique of determining the informants used was purposive sampling.
The results showed that the existing conditions on Dream Island Beach based on Concept 4a were still lacking. The condition of the attraction is very good but for access and facilities still, have some disadvantages that need to be improved in the future. The reality that is on Dream Island Beach only consists of private parties and village institutions. The marketing strategy at Dream Island Beach has not been optimal due to several problems such as business license constraints and lack of cooperation with promotional media.

Keywords: Marketing Strategic, attraction, Dream Island Beach
Analisis Kepuasan Wisatawan Terhadap Produk Wisata dan Strategi Pengembangan DTW Telaga Wopersnondi Biak Timur
Authors: Salakory Hans S. M. , Matulessy Febby Sonya , Saragih Yansen M. I 
Published: December 2020

Visitors of Wipersnondi Lake had great hope of fulfilling their comfort when they arrived at the tourism sites, in fact the performance felt by visitors cannot satisfy all the expectations of visitors and had impact on their satisfaction and convenience. This study aims to determine the level of satisfaction of tourist visitors to the availability of elements of tourism products, and to find out its developments strategy. To answer the research problem, the analysis technique used is Customer Satisfaction Index (CSI) and Importance Performance Analysis (IPA). The results of research based on CSI show that visitors are not satisfied with the performance of tourism products at Wopersnondi Lake. Based on the results of the IPA analysis, the main priorities for tourism development in Wopersnondi Lake are increasing affordability and opening of public transport routes, improving cleanliness and increasing the number of toilets, specifically at the lake location, providing road directions to the location, and building health facilities like public health centers around Wopersnondi Lake. Furthermore, the unique aspects of Wopersnondi Lake that must be maintained are the beautiful view at the tourist location, the comfort of the tourist attraction, the coolness, the cleanliness, the quality of the road to the lake, the parking lot area, and the availability of trash cans are things that have actually been felt and have created comfort and good experience for visitors to Wopersnondi Lake. This development must support the development of other tourism products..
Key Word : Importance Performance Analysis, Customer Satisfaction Index.
Peluang Pengembangan Voluntourism Berbasis Masyarakat di Hutan Mangrove Batu Lumbang, Suwung, Pemogan, Denpasar
Authors: Tyas Septianing , Arida I Nyoman Sukma 
Published: December 2020

The Mangrove Batu lumbang as conservation areas where live so many variety of flora and also has so beautiful view. Although the mangrove has not yet defined it as a tourist attraction, there are few tourist visitors. Assets or potential have been particularly supportive to develop as alternative tourism but there are some issues about garbage and waste. Voluntourism has become one of the best types of alternative tourism to develop. Voluntourism or volunteer tourism is a form of tourism where tourists use leisure time as volunteers and donators on conservation projects in an attraction. The purpose of this study is to identify the chances of voluntourism development in the mangrove forest Batu Lumbang based communities to help the ecosystem and grow the communities also give a new experience for tourist. The study used qualitative descriptive methods. The result of this study is the mangrove forest Batu Lumbang fillup some of the criteria of voluntourism activity. The criteria of the study based on the criteria of the global tourism business industries. The limits of this research are the time to finding data due to a covid-19 outbreak.

Keyword: voluntourism; Hutan Mangrove Batu Lumbang; Responsible Tourism
Tingkat Kepuasan Wisatawan Terhadap Kualitas Sarana dan Prasarana di Kawasan Kota Lama Semarang
Authors: Puspitasari Meike Lintang , Sastrawan I Gede Anom 
Published: December 2020

This study aims to determine the level of tourist satisfaction with the quality of facilities and infrastructure in the Semarang Old City Area. Revitalization of the Semarang Old City Area is done by improving infrastructure and adding facilities and infrastructure. Good quality facilities and infrastructure can produce satisfaction for tourists who come. This research uses qualitative and quantitative data types, while the data sources used are primary and secondary sources. Data collection techniques used in this study were observation, questionnaires, and interviews. The measurement scale of variables uses the Likert scale. The technique of determining informants using purposive sampling. The data analysis technique used is quantitative descriptive. The results of this study are tourists most satisfied with pedestrian road and tourist most dissatisfied with bench and parking area. The condition of good facilities and infrastructure will produce satisfaction for tourists. The results of the analysis can be used as an evaluation for the manager of Semarang Old City Area.

Keyword: condition, quality, facilities, infrastructure, and satisfaction level.
BOLU İLİNDE DEMOGRAFİK KARAKTERİSTİKLERİN ERKEK HEMŞİREYE YÖNELİK TUTUMLAR ÜZERİNDEKİ ETKİSİ
Authors: SUVEREN Eylem , ALÇİN Alev , ÇUKUR Kübra , KORKMAZ Özge , ÜNAL Melike 
Published: December 2020

Amaç: Kadın hemşirelerden hemşirelik hizmetini almaya alışkın toplumda, erkek hemşireden bakım alan hastaların erkek hemşirelere olan tutumlarının incelenmesidir. Gereç ve Yöntemler: Tanımlayıcı tipte planlanan çalışmada evreni, Abant İzzet Baysal Üniversitesi Eğitim ve Araştırma Hastanesi Tıp Fakültesi, Cerrahi ve Dahiliye ünitelerinde yatmakta olan, en az bir kez erkek hemşireden bakım alan, mental ya da psikiyatrik hastalığı olmayan 219 gönüllü hasta oluşturmuş ve olasılıksız örneklem tekniği ile araştırma kapsamına alınmıştır. Anket formu, katılımcıların sosyo-demografik ve klinik özellikleri ile erkek hemşirelere yönelik tutumlarına ilişkin Likert tipte, 22 önermeden oluşmaktadır. Niteliksel verilerin karşılaştırılmasında Pearson Ki-kare testi kullanılmış, sonuçlar %95 güven aralığında, p&lt;0,05 anlamlılık düzeyinde değerlendirilmiştir.Bulgular: Araştırmada, kadınların %72,6'sı, erkeklerin %27,4’ü erkek hemşireden zorunlu olmadıkça hizmet almak istemediğini belirtmiştir (p&lt;0,00). Kadınların %67,4’ü, erkeklerin %32,6’sı mahrem olduğunu düşündüğü fikrini erkek hemşireye söylemeyi istemediğini ifade etmiştir (p&lt;0,00). Erkek hemşireler, erkek hastalar tarafından kabul görürken (%54,7) kadın hastalar (%45,3) çekingenlik sergilemiştir (p&lt;0,05). Eğitim durumu yüksek olan hastaların erkek hemşirelere olumlu tutum gösterdiği bulunmuştur (p&lt;0,00).Sonuç: Cinsiyet ayrımcılığı olmaksızın icra edilen hemşirelik mesleğinin, başta erkek öğrenciler olmak üzere toplumun tamamına ulaştırılması ve devletin bilinçlendirme politikalarında hedef merkezli stratejiler benimsenerek negatif tutumların yoğun algılandığı gruplarda farkındalığı artırıcı çalışmalar önerilmektedir.
Querefonte, o amigo mais desvairado de Sócrates.
Authors: Agostini Cristina De Souza 
Published: December 2020

Neste artigo, apresento uma análise acerca dos elementos que, na Apologia e no Cármides, de Platão, ligam os personagens Sócrates e Querefonte no que concerne à prática dialógica filosófica. Assim, pretendo demonstrar que, de acordo com uma argumentação que parte desses dois textos, de um lado, Querefonte foi figura imprescindível para que Sócrates estabelecesse o diálogo como essência de sua filosofia, na medida em que a consulta que fizera ao oráculo de Delfos foi o ponto de partida para que a investigação socrática tal como a que temos delineada nos diálogos se concretizasse. De outro lado, diferentemente dos seguidores de Sócrates, Querefonte é um personagem que apresenta dedicação à filosofia com certa independência intelectual em relação ao seu amigo, manifestando, assim, ações que expressam a liberdade de pensamento que desfruta e, portanto, o coloca em pé de igualdade a Sócrates. Nesse sentido, caracterizar Querefonte como filósofo tal qual Sócrates pode nos fazer avançar na compreensão sobre o uso do diálogo socrático como ferramenta pedagógico-filosófica, destinada aqueles que, diferentemente de Querefonte, não são capazes ainda de filosofarem por si mesmos.
Do prophylactic steroids prevent chemical meningitis in surgery for epidermoid cysts? Case report and literature review
Authors: Ganko Renata , Rodriguez Michael , Magnussen John , Simons Mary , Myint Esther , Assaad Nazih 
Published: December 2020

Background:
Cranial and spinal epidermoid cysts (ECs) are rare and surgical resection can be complicated by chemical meningitis. Here, we treated a patient undergoing surgical resection of an intramedullary spinal EC with prophylactic steroids to help prevent postoperative chemical meningitis. Notably, we found a paucity of evidence regarding the efficacy of steroids used for this purpose.

Case Description:
A 44-year-old male presented with a rare intramedullary thoracic EC. He was given oral dexamethasone postoperatively and did not subsequently develop chemical meningitis. Here, we reviewed the current literature regarding the efficacy of steroid use for this purpose, utilizing multiple electronic databases (Ovid MEDLINE, Ovid EMBASE, and Scopus). We found only three studies (one case report, one case series, and a randomized controlled trial), that involved patients who received steroids. Of the 24 patients given prophylactic steroids, none developed fever or meningismus. One patient received 8 days of oral dexamethasone. Eleven patients received intraoperative hydrocortisone irrigation alone, while final 12 patients received intraoperative hydrocortisone irrigation plus a 3 week postoperative tapering course of oral steroids. Notably, all of the nine patients who did not receive any steroids developed postoperative fever, with 78% demonstrating meningismus.

Conclusion:
Here is level II evidence that establishes the efficacy of prophylactic steroids utilized in patients undergoing surgery for ECs to prevent postoperative chemical meningitis. Nevertheless, there is still no current consensus regarding either the type of steroid utilized, or the route of administration.
Hemorrhage in long segment cervical schwannoma; case report and literature review
Authors: Singh Prashant Raj , Nayak Nitish , Gupta Surendra Kumar , Sharma Raghavendra Kumar , Shukla Anju , Nehete Lokesh Suresh 
Published: December 2020

Background:
Although hemorrhages associated with cervical and thoracic intraspinal schwannomas are typically localized to the subarachnoid hemorrhages (SAH) or subdural hemorrhages (SDH) compartments, rare intratumoral bleeds may also occur.

Methods:
In the literature, we found and analyzed multiple factors for 13 cases (e.g., epidemiological, clinical, and pathological) of cervical schwannomas with intratumoral hemorrhages (ITH). We added the 14th case of a 35-year-old female with along segment cervical schwannoma with ITH who presented with acute quadriplegia and respiratory decompensation.

Results:
These 14 patients averaged 51.77 years of age, 60% were male, and the tumor involved 2.83 segments. The incidence of SAH and ITH was noted in five cases each, while SDH’s were very rare. The pathological characteristics were consistent with the diagnosis of cellular schwannomas with S-100 positivity. The clinical outcomes were good (100%) in all the cases, including the one presented (modified McCormick score III).

Conclusion:
Cervical schwannomas with ITH are rare, and the surgical outcomes in such patients are good-excellent (>90%). The histopathology is always of prime importance and decisive in establishing and confirming the etiology of such ITH.
Significance of the «youth protein», GDF11, and «aging proteins», CCL11, GDF15, and JAM-A, for regulation of blood pressure in healthy and hypertensive women
Authors: Кузник Б.И. , Давыдов С.О. , Степанов А.В. , Смоляков Ю.Н. , Гусева Е.С. 
Published: December 2020

Цель исследования - изучение взаимосвязи содержания белков GDF11, GDF15, CCL11, JAM-A и кровяного давления у здоровых женщин и женщин, страдающих гипертонической болезнью. Методика. В исследовании приняло участие 118 женщин. Содержание белков GDF11, GDF15, JAM-A и CCL11 определялось методом иммуноферментного анализа (ИФА). Вычислялись коэффициенты, характеризующие отношение «белка молодости» к «белкам старости» GDF11/GDF15, GDF11/CCL11 и GDF11/JAM-A. Величину систолического (SBP) и диастолического давления (DBP) определяли методом Короткова. Результаты. У женщин, страдающих гипертонической болезнью, значительно снижено содержание «белка молодости» GDF11 и повышена концентрация «белков старости» - GDF15, CCL-11 и адгезивной молекулы JAM-A. Установлены положительные и отрицательные корреляции между отдельными исследуемыми компонентами и уровнем кровяного давления. Показано, что чем выше показатель отношения «белка молодости» GDF11 к «белкам старости» GDF15, CCL-11, JAM-A, тем ниже уровень систолического, диастолического и среднего кровяного давления. Заключение. На основании полученных данных сделано заключение, что соотношения уровней «белка молодости» GDF11 и «белков старости» - GDF15, CCL11 и JAM-A играет в регуляции уровня кровяного давления существенно более важную роль, чем каждая из этих молекул в отдельности. Систематическое использование на протяжении 2-3 лет курсов кинезитерапии приводит к восстановлению нормального соотношения «белков молодости» и «белков старости», что сопровождается нормализацией кровяного давления.
The aim of this work was to study the relationship between contents of GDF11, GDF15, CCL11, and JAM-A proteins and blood pressure in healthy women and women with essential hypertension. Methods. The study involved 118 women. Contents of GDF11, GDF15, JAM-A, and CCL11 were measured using the enzyme immunoassay (ELISA). Ratios of the «youth protein» to «aging proteins» (GDF11/GDF15, GDF11/CCL11, and GDF11/JAM-A) were computed. Systolic (SBP) and diastolic (DBP) blood pressure was measured using the Korotkov method. Results. In hypertensive women, the content of the «youth protein», GDF11, was significantly reduced, and concentrations of «aging proteins», GDF15, CCL-11, and the adhesion molecule JAM-A, were increased. Some «youth and aging proteins» were positively or negatively correlated with blood pressure. The greater was the ratio of the «youth protein», GDF11, to «aging proteins», GDF15, CCL-11, and JAM-A, the lower was systolic, diastolic, and mean blood pressure. Therefore, the relationship between the ratio of the «youth protein», GDF11, to «aging proteins», GDF15, CCL11, and JAM-A, plays a substantially greater role in regulation of blood pressure than each of these molecules alone. Conclusion. Systematic use of kinesiotherapeutic courses for 2-3 years restores a normal «youth to aging protein» ratio and improves blood pressure.
Screening test for determination of multiple-modified lipoproteins of low density - marker of subclinical atherosclerosis
Authors: Шойбонов Б.Б. , Драпкина О.М. , Елиашевич С.О. , Лавренова Е.А. , Федорович А.А. , Сухинина Н.Ю. , Толпыго С.М. , Григорьева Д.В. , Баронец В.Ю. , Панченко Л.Ф. , Кубатиев А.А. 
Published: December 2020

Разработан скрининг-тест для определения множественно-модифицированных липопротеинов низкой плотности (ммЛПНП) в сыворотке крови для рутинных исследований. Инкубация сыворотки в буфере, содержащем 9,1% поливинилпирролидона 35000 (ПВП-35) приводит к полной агрегации ммЛПНП. Патогенность выделенных ммЛПНП подтверждена в тестах связывания системы комплемента и литической активности in vitro , а также в тесте сосудистой дистонии у крыс in vivo . Предлагаемый способ отличается высокой производительностью и низкой себестоимостью. Метод включает 2 операции - смешивание сыворотки с раствором 10% ПВП-35 в 96-луночных иммунологических планшетах, инкубация в течение 10 мин при комнатной температуре и турбидиметрия при 450 нм на ИФА-ридере. Проведены исследования взаимосвязи концентрации ммЛПНП в сыворотке крови и толщины комплекса интима-медиа по данным ультразвукового дуплексного сканирования сонных артерий у 78 человек с низким сердечно-сосудистым (SCORE <1%) и низким кардио-метаболическим рисками (CMDS 0-1). Из всех фракций липидного спектра крови ммЛПНП показали самый высокий коэффициент корреляции с показателями субклинического атеросклероза, что подтверждает высокий атерогенный потенциал ммЛПНП.
A screening test was developed to determine multiply-modified low-density lipoprotein (mmLDL) in serum for routine studies. Incubation of serum in a buffer containing 9.1% polyvinylpyrrolidone 35,000 (PVP-35) results in complete aggregation of mmLDL. Pathogenicity of isolated mmLDL was confirmed during binding tests of the complement system and lytic activity in vitro , and also in vascular dystonia test with rats in vivo . The proposed method is characterized by high productivity, low cost, includes 2 operations - mixing serum with a solution of 10% PVP-35 in 96-well immunoassay plates, incubating for 10 min at room temperature and turbidimetry at 450 nm on an ELISA reader. A study was made to determine relationship between concentration of mmLDL in the blood serum and thickness of the intima-media complex from data of ultrasound duplex scanning of carotid arteries with 78 people with low cardiovascular risk (SCORE <1%) and low cardiovascular risk (CMDS 0-1). Of all fractions of the lipid spectrum of the blood, mmLDL showed the highest correlation coefficient with the indices of subclinical atherosclerosis, which confirms high atherogenic potential of mmLDL.