bluuebunny/crossref_metadata_2025_split huggingface dataset:
Blood component therapy for dry eye disease: a systematic review and network meta-analysis
Authors: Zhang Yu , Li Ning , Ge Ziying , Li Fang 
Published: December 2024

ObjectiveBlood component therapy has shown promising potential as an emerging treatment for dry eye disease; however, it remains unclear which specific blood component is the most effective. This study aims to compare the efficacy of different blood components in the treatment of dry eye disease through a network meta-analysis, with the goal of providing the latest and most reliable evidence for clinical practice.MethodsWe conducted a systematic search of the PubMed, Web of Science, Cochrane, Embase, and Scopus databases, with the search concluding on June 1, 2024. Two independent researchers performed literature screening, data extraction, and quality assessment.ResultsA total of 16 randomized controlled trials (RCTs) involving 898 patients with dry eye disease were included. Six different blood components were utilized in treating dry eye disease, with platelet-rich plasma (PRP) being the most widely used. The results of the network meta-analysis indicated that platelet-rich plasma eye drops (PRPD) significantly outperformed artificial tears (AT) in improving the corneal fluorescein staining score (CFSS), while autologous serum (ALS) and umbilical cord serum (UCS) also demonstrated significantly better effects than AT in enhancing tear break-up time (TBUT). Additionally, ALS, PRP injection (PRPI), and PRPD showed significantly superior outcomes compared to AT in improving the ocular surface disease index (OSDI). However, no statistically significant differences were found among the various treatment modalities regarding their effects on Schirmer’s I value, CFSS, TBUT, and OSDI. SUCRA analysis predicted that UCS was the most effective in improving Schirmer’s I value and TBUT, while PRP excelled in enhancing CFSS and OSDI. Limitations such as publication bias and issues related to randomization, allocation concealment, and blinding may affect the reliability of the current findings.ConclusionBlood component therapy can significantly improve the pathological damage and ocular surface health in patients with dry eye disease. For those with aqueous-deficient dry eye, UCS may represent the optimal treatment option. In contrast, for patients with more severe corneal epithelial damage, PRP may offer a more effective therapeutic approach.Systematic Review Registrationhttps://www.crd.york.ac.uk/PROSPERO/, CRD42024534091.
Whole-genome sequencing and genomic analysis of four Akkermansia strains newly isolated from human feces
Authors: Lu Wenjing , Zha Biqing , Lyu Jie , LingHu Chenxi , Chen Jing , Deng Sisi , Zhang Xiangling , Li Liang , Wang Guoqing 
Published: December 2024

BackgroundNumerous studies have demonstrated that Akkermansia is closely associated with human health. These bacteria colonize the mucus layer of the gastrointestinal tract and utilize mucin as their sole source of carbon and nitrogen. Akkermansia spp. exhibit potential as probiotics under specific conditions. However, the gene accumulation curve derived from pan-genome analysis suggests that the genome of Akkermansia strains remains open. Consequently, current genome mining efforts are insufficient to fully capture the intraspecific and interspecific characteristics of Akkermansia, necessitating continuous exploration of the genomic and phenotypic diversity of new isolates.MethodsBased on this finding, we sequenced, assembled, and functionally annotated the whole genomes of four new human isolates from our laboratory: AKK-HX001, AKK-HX002, AKK-HX003, and AKK-HX004.ResultsPhylogenetic analysis revealed that all four isolates belonged to the AmII phylogroup, whereas the type strain DSM 22959 is classified within the AmI phylogroup. Moreover, 2,184 shared homologous genes were identified among the four isolates. Functional annotation using the COG, KEGG, and CAZy databases indicated that the functional genes of the four isolates were primarily associated with metabolism. Two antibiotic resistance genes were identified in AKK-HX001 and AKK-HX002, while three resistance genes were detected in AKK-HX003 and AKK-HX004. Additionally, each of the four isolates possessed two virulence genes and three pathogenicity genes, none of which were associated with pathogenicity. The prediction of mobile genetic elements indicated unequal distributions of GIs among the isolates, and a complete CRISPR system was identified in all isolates except AKK-HX003. Two annotated regions of secondary metabolite biosynthesis genes, both belonging to Terpene, were detected using the antiSMASH online tool.ConclusionThese findings indicate that the four Akkermansia isolates, which belong to a phylogroup distinct from the model strain DSM 22959, exhibit lower genetic risk and may serve as potential probiotic resources for future research.
Aftereffect of single transcranial direct and alternating current stimulation on spontaneous home-cage and open-field EEG activities in a mouse model of Alzheimer’s disease
Authors: Sun Huaying , Wang Yumei , Yuan Dong , Duan Mengsi , Chen Zhuangfei , Fu Yu 
Published: December 2024

BackgroundAs a non drug and non invasive therapy, both transcranial alternating current stimulation (tACS) and transcranial direct current stimulation (tDCS) may modulate cortical rhythms and serve as potentially effective approaches to cognitive decline in Alzheimer’s disease (AD). However, studies using animal models of AD are quite limited.MethodsThis study investigates the aftereffects of tACS and tDCS on brain EEG activity and associated exploratory behavior in normal aged and APP/PS1 transgenic mice (15 months old). Anodal tDCS and 10 Hz tACS (350 μA, 20 min) were applied once and EEGs were recorded from the hippocampus (Hip) and prefrontal cortex (PFC) during spontaneous home-cage state and open-field exploration.ResultsA key finding was that tDCS induced significant alpha (8–12 Hz) EEG changes while tACS induced peak frequency changes in the group difference between normal aged and AD mice. However, both groups showed similar increases in theta (4–8 Hz) EEG activity during open-field exploration and increases in gamma (20–100 Hz) EEG activity in spontaneous state, suggesting that the ongoing physiological state may be related to some of the EEG changes.ConclusionThis study provides insight into the short-term aftereffects of transcranial current stimulation in the aging and AD brain and is the first animal study to compare brain activity between tACS and tDCS treatments.
Adolescent circadian rhythm disruption increases reward and risk-taking
Authors: DePoy Lauren M. , Vadnie Chelsea A. , Petersen Kaitlyn A. , Scott Madeline R. , Zong Wei , Yin RuoFei , Matthaei Ross C. , Anaya Fernanda Juarez , Kampe Callie I. , Tseng George C. , McClung Colleen A. 
Published: December 2024

IntroductionCircadian rhythm disturbances have long been associated with the development of psychiatric disorders, including mood and substance use disorders. Adolescence is a particularly vulnerable time for the onset of psychiatric disorders and for circadian rhythm and sleep disruptions. Preclinical studies have found that circadian rhythm disruption (CRD) impacts the brain and behavior, but this research is largely focused on adult disruptions. Here, we hypothesized that adolescent CRD would have a greater effect on psychiatric-related behaviors, relative to adult disruption.MethodsWe determined the long-term behavioral and neurobiological effects of CRD during early adolescence by exposing mice to 12 h shifts in the light/dark cycle. Adult mice were exposed to the same CRD paradigm. Behavior testing began approximately 4 weeks later for both groups. To identify possible mechanisms, we also measured gene expression in brain regions relevant to circadian rhythms, mood and reward.ResultsCRD during early adolescence, but not adulthood, persistently increased exploratory drive (risk-taking behavior) and cocaine preference when tested later in life. Interestingly, we found sex differences when intravenous cocaine self-administration was tested. While female mice with a history of adolescent CRD had a greater propensity to self-administer cocaine, as well as increased motivation and cue-induced reinstatement, male adolescent CRD mice had reduced motivation and extinction responding. Importantly, we found that transcripts in the SCN were affected by adolescent CRD and these were largely distinct across sex.ConclusionOverall, adolescent CRD in mice caused persistent increases in risky behavior, cocaine reward and cocaine self-administration, which suggests that CRD during adolescence may predispose individuals toward substance use disorders. Future research is required to elucidate how adolescent CRD affects behaviors relevant to mood-and substance use-related disorders across the 24-h day, as well as to identify intervention strategies to alleviate disruption during adolescence and novel therapeutic approaches once symptoms have begun.
Annao Pingchong decoction attenuates oxidative stress and neuronal apoptosis following intracerebral hemorrhage via RAGE-NOX2/4 axis
Authors: Wang Xu , Lin Xiaoyuan , Chen Zilin , Long Hongping , Zhou Xuqing , Lei Shihui , Liu Jian , Dong Huan , Liu Fang , Hu Hua , Guo Chun 
Published: December 2024

BackgroundIntracerebral hemorrhage (ICH) is a severe condition associated with high mortality and disability rates. Oxidative stress plays a critical role in the development of secondary brain injury (SBI) following ICH. Previous research has demonstrated that Annao Pingchong decoction (ANPCD) treatment for ICH has antioxidant effects, but the exact mechanism is not yet fully understood.ObjectiveThis study aimed to investigate the neuroprotective effects of ANPCD on oxidative stress and neuronal apoptosis after ICH by targeting the receptor for advanced glycation end products (RAGE)-NADPH oxidase (NOX) 2/4 signaling axis.MethodsThe research involved the creation of rat ICH models, the mNSS assay to assess neurological function, Nissl staining to evaluate neuronal damage, and biochemical assays to measure oxidative and antioxidant levels. The expression of RAGE-NOX2/4 axis proteins was analyzed using western blotting and immunofluorescence, while neuronal apoptosis was assessed with TUNEL staining. Furthermore, after performing quality control of drug-containing serum using UPLC-MS/MS, we employed an in vitro model of heme-induced injury in rat cortical neurons to investigate the neuroprotective mechanisms of ANPCD utilizing RAGE inhibitors.ResultsThe findings indicated that ANPCD improved neurological deficits, reduced neuronal damage, decreased ROS and MDA levels, and increased the activities enzymatic activities of SOD, CAT, GSH and GPX. Additionally, it suppressed the RAGE-NOX2/4 signaling axis and neuronal apoptosis.ConclusionANPCD exhibits neuroprotective effects by inhibiting the RAGE-NOX2/4 signaling axis, thereby alleviating neuronal oxidative stress and apoptosis following ICH.
Prognostic value of the triglyceride—glucose index in non-muscle-invasive bladder cancer: a retrospective study
Authors: Zhang Yan , Shao Xianfeng , Ding Li , Xia Wentao , Wang Kun , Jiang Shan , Wang Jiahao , Wang Junqi 
Published: December 2024

BackgroundBladder carcinoma is a type of urological tumor with high risks of recurrence and progression. The triglyceride-glucose (TyG) index has demonstrated significant promise as a prognostic marker for metabolic health in different types of cancer. Further research is needed to explore the relationships among non-muscle-invasive bladder cancer (NMIBC), the TyG index, and its prognostic importance. Purpose of this preliminary research is to assess the predictive significance of the TyG index for recurrence and progression risk in NMIBC patients.MethodsData from patients admitted between October 2018 and July 2021 were reviewed, and there are 198 patients in total were included. The experimental data were supplied by medical records. In addition, patient prognoses were followed up via telephone. Furthermore, patients were separated into two groups: the high and low TyG groups, using X-tile software. Apart from recurrence-free survival (RFS), progression-free survival (PFS) was the main outcome. According to the TyG index, nomograms were also established.ResultsThe cohort consisted of 93 patients in the high TyG group and 105 patients in the low TyG group. The TyG index was a key prognostic factor for postoperative RFS (HR = 2.726, 95% CI = 1.474–5.041, p = 0.001) and PFS (HR = 2.846, 95% CI = 1.359–5.957, p = 0.006) among patients with NMIBC. The log-rank test revealed a notable disparity between the low and high TyG groups regarding RFS (p = 0.0025) and PFS (p = 0.0110). Moreover, it was strongly connected to well-known NMIBC risk factors. Because the TyG index exhibited good predictive value, the nomogram models were formulated.ConclusionThe TyG index serves as an isolated predictor of both RFS and PFS among patients with NMIBC, revealing new insights into disease treatment mechanisms. Indeed, the TyG index serves as a credible indicator of risk classification while facilitating early intervention among patients with NMIBC.
RNA-seq profiling identified a three-lncRNA panel in serum as potential biomarker for muscle-invasive bladder cancer
Authors: Jiang Xiumei , Qu Ailin , Zhang Shoucai , Jin Shuchao , Wang Lishui , Zhang Yi 
Published: December 2024

BackgroundPreoperative determination of muscular infiltration is crucial for appropriate treatment planning in patients with muscle-invasive bladder cancer (MIBC). We aimed to explore early diagnostic biomarkers in serum for MIBC in this study.MethodsThe expression profiles of long noncoding RNA (lncRNA) were initially screened by high-throughput sequencing and evaluation of potential lncRNAs were conducted by two phases of RT-qPCR assays using serum samples from 190 patients with MIBC and 190 non-muscle-invasive BC (NMIBC) patients. Multivariate logistic regression analysis was applied to establish a diagnostic signature with high accuracy and Fagan’s nomogram was plotted to promote clinical application. Bioinformatics analysis was used to determine the potential miRNA-mRNA binding of candidate lncRNAs.ResultsWe identified three differentially expressed lncRNAs (LINC00565, LINC00592 and NDUFA6-AS1) and established a 3-lncRNA panel which demonstrated high diagnostic accuracy for MIBC with an AUC of 0.903 (95% CI: 0.850-0.942) and 0.875 (95% CI: 0.802-0.928) in the training and validation set. Moreover, construction and assessment of Fagan’nomogram demonstrated that the 3-lncRNA panel could exhibit practical and helpful values for clinical use. Finally, a network map based on LINC00565 was constructed and we found that the expression of miR-143-5p and miR-4516 were significantly correlated with LINC00565 in MIBC.ConclusionOur findings indicated that the constructed 3-lncRNA panel in serum showed favorable diagnostic capacity and might serve as promising non-invasive biomarkers in the early diagnosis of MIBC.
Effectiveness of continuity of care in postoperative patients with cervical cancer: a systematic evaluation and meta-analysis of a randomized controlled trial
Authors: Fu Wenfeng , Xu Qiannan , Lu Haizhen , Yang Yuanyuan , Zheng Yanxue 
Published: December 2024

ObjectiveTo analyze the effect of implementing continuity of care for postoperative patients with cervical cancer, to improve the continuity of care model in China, and to provide comprehensive, continuous, and personalized care services for patients.MethodsPubMed, Web of Science, Science Direct, China Knowledge Network, Wanfang database, China Biomedical sources Service System, Wipro, Cochrane Library, Embase, and other databases were searched for relevant sources on the effect of continuity of care on postoperative cervical cancer patients. The Cochrane Handbook of Systematic Evaluation and Revman 5.3 software was used to evaluate the quality of the retrieved sources and perform meta-analysis.ResultsCompared with the control group, extended care could improve patients’ quality of life [SMD=1.35,95%CI(1.05,1.64), P&lt;0.05], alleviate patients’ postoperative anxiety [SMD=-0.92,95%CI(-1.85,0.00), P&lt;0.05] and postoperative depression [SMD=-1.15,95% CI(-1.35,-0.95), P&lt;0.05].ConclusionThe use of continuity of care form has an important role in improving the quality of life of cervical cancer patients and improving negative emotions.
Updated cost-effectiveness analysis of tislelizumab in combination with chemotherapy for the first-line treatment of advanced gastric cancer or gastroesophageal junction adenocarcinoma
Authors: Xu Lei , Long Yunchun , Yao Lu , Wang Hao , Ge Weihong 
Published: December 2024

ObjectiveThe RATIONALE-305 trial demonstrated that tislelizumab in combination with chemotherapy regimens was more beneficial than chemotherapy regimens alone in the treatment of patients with advanced gastric cancer or gastroesophageal junction adenocarcinoma (GC/GEJC). This study aimed to evaluate the cost-effectiveness of tislelizumab combination chemotherapy in the treatment of advanced GC/GEJC from the perspective of the Chinese health service system.MethodsA three-state partition survival model was constructed to evaluate the economics of tislelizumab combined with chemotherapy as the first-line treatment of advanced GC/GEJC. Clinical data were collected from the RATIONALE-305 trial, and the incremental cost-effectiveness ratio (ICER) was calculated using quality-adjusted life years (QALYs) as the output index. The stability of the results was verified using sensitivity and subgroup analyses. In addition, scenario analysis was conducted for the model simulation time and different parameter extrapolation models.ResultsThe results of basic analysis showed an increase of 0.31 QALYs in the tislelizumab group compared with the placebo group (1.53 QALYs vs 1.22 QALYs), and a concomitant increase in cost of 10,326.68 USD, with an ICER of 33,876.38 USD/QALY, which is less than the current Chinese willingness-to-pay threshold (36,924.80 USD/QALY). Sensitivity analyses demonstrated that the utility values of progression-free survival, progressive disease and the price of capecitabine had a greater impact on the model. Subgroup analysis revealed that combination therapy was equally cost-effective in people with a program death ligand 1 tumor area positivity score of ≥5%.ConclusionFrom the perspective of the Chinese health service system, the treatment of advanced GC/GEJC with tislelizumab combined with chemotherapy has a cost-effective advantage over chemotherapy alone.
Use of non-specific immunoglobulins in Catalonia in three third-level hospitals: a descriptive analysis of a hospital-prescribed medication registry
Authors: Riera-Arnau J. , Ballarín E. , Llop R. , Montané E. , Hereu P. , Vancells G. , Padullés-Zamora N. , Barriocanal A. M. , Cardona-Peitx G. , Casasnovas C. , Montoro J. B. , Nuñez M. , Santacana Juncosa E. , Selva-O’Callaghan A. , Solanich X. , Sabaté Gallego M. 
Published: December 2024

BackgroundThe increasing use of non-specific immunoglobulins (NSIGs) and their current shortage show a need for NSIGs’ use prioritization. Data from a clinical perspective are necessary, mainly for pediatric patients.ObjectivesThe aim of the study was to assess the level of clinical evidence (LoE) of the indications that NSIGs are used for, the reasons for discontinuation, and the costs invested.MethodsA retrospective multicentric study was conducted on NSIG incident users between September 2019 and December 2021 retrieved from the Registry of Patients and Treatments (RPT) from Catalonia (Spain). LoE was categorized as A) authorized indications, B) unauthorized with scientific support, C) unauthorized without support, and D) unknown (UNK), following local and the United Kingdom’s guidelines as a sensitivity analysis. We also estimated overall spending and costs per patient visit.ResultsA total of 400 patients were included (17.3% pediatric), with a mean follow-up of 122.1/person-years for adults. The most frequent indications were nervous system and blood diseases. Almost all pediatric patients (56; 81.2%) were treated under A-level indications, as for 217 (65.6%) adults. In the sensitivity analysis, the A-level usage rate decreased to one-third and the B-level usage rate increased by 2–3 times. Furthermore, 37.8% (151) of individuals discontinued. This was predominantly due to remission or no response. The total costs were 868,462.6€/year, with median spending per visit amounting to 1,500€ for adults and 700€ for pediatric patients.ConclusionNSIGs are used in clinical practice mainly for approved indications; however, non-approved indications are still an important issue. This could represent a significant economic burden on the healthcare system, focusing on the pediatric population and those at risk for discontinuation with alternative therapeutic options.
Light quality regulates growth and flavonoid content in a widespread forest understorey medicinal species Scutellaria Baicalensis Georgi
Authors: Ma Jingran , Zhang Jiaxing , Xie Lulu , Ye Ji , Zhou Li , Yu Dapao , Wang Qing-Wei 
Published: December 2024

IntroductionIntroduction: Light is not only essential for plant photosynthesis and growth, but also acts as a signal to regulate its secondary metabolism. Despite the influence of light quality on the yield and flavonoid compounds in commercial crops is well-documented, its role in regulating wild understorey species, particularly medicine plants whose flavonoid biosynthesis driven by multiple spectral regions of canopy sunlight, is less understood.MethodsTo address it, we conducted a light-quality manipulation experiment on Scutellaria baicalensis Georgi, a widespread understorey medicinal species, with light-emitting diodes (LED). This study included eight treatments: UV-A (UV-A radiation), CK (control group), Green (monochromatic green light), and different combinations of blue and red light (R0B4: monochromatic blue light; R1B3: 25% Red+75% Blue light; R1B1: 50% Red+50% Blue light; R3B1: 75% Red+25% Blue light; R4B0: monochromatic red light).ResultsOur results showed that light quality significantly drove morphology, biomass accumulation, and flavonoids biosynthesis in S. baicalensis. R0B4 treatment promoted growth and flavonoids accumulation, including baicalin, and wogonoside concentrations. In contrast, UV-A radiation and green light negatively impacted these parameters compared to CK treatment. Interestingly, plant biomass and flavonoid concentrations were lower in R1B3, R1B1 and R3B1 treatments compared to monochromatic blue or red light.DiscussionOur study found that red light may antagonize blue light-stimulated growth and flavonoids accumulation, indicating a complex crosstalk between photoreceptors. These findings highlight the importance of blue light for optimizing the yield and quality of S. baicalensis in the understorey cultivation. It provides practice suggestion for the efficient management and sustainable cultivation of understorey medicinal plants.