bluuebunny/crossref_metadata_2025_split huggingface dataset:
In vitro callus and shoot regeneration in Enterolobium cyclocarpum (Jacq.) Grised. - a fast timber yielding species
Authors: AKINROPO Michael S. , AYISIRE Benjamin E. , OGBIMI Ejeoghene R. 
Published: March 2020

This study was conducted to investigate the in vitro callus induction and rapid shoot regeneration potential in Enterolobium cyclocarpum, a plant native to central Mexico but widely introduced into Africa. The leaf, stem and nodal explants of E. cyclocarpum were cultured on full strength Murashige and Skoog (MS) medium supplemented with different concentrations of Cytokinins - Benzyladenine (BA) and/or Kinetin and Auxins - Naphthalene acetic acid (NAA) and/or 2,4-Dichlorophenoxylacetic acid (2,4-D) each alone and in combination. The leaf explants did not respond to these treatments. The Nodal explants were best for caulogenesis, while the explant responses were in the order- nodal > stem > cotyledon for callogenesis in MS medium supplemented with BA and/or Kin combined with NAA and/or 2,4-D. The varied combinations induced white compact callus. The highest callus production was observed on MS medium supplemented with 2.7 µM NAA + 2.2 µM BA and 5.4 µM NAA alone. Nodal and cotyledon explants developed callus and multiple shoots on MS supplemented with a combination of cytokinin (BA and/or Kin.) and auxin (NAA and/or 2,4-D). The maximum number of 3.98 ± 0.37 and 2.1±0.11 shoots/explants were recorded for nodal and cotyledon explants on MS medium supplemented with a combination of 8.8 µM BA+2.7 µM NAA and 2.2µM BA+2.7 µM NAA respectively. On the basal medium, 10% of the excised shoots rooted successfully. Thus, this in vitro method can be exploited for conservation and mass propagation of this fast timber yielding tree and also utilized for embryogenesis studies.
Clinical and epidemiological study of non-venereal genital diseases in females
Authors: P. Mamatha , Arora Sheena , Vardhan Abhijeet 
Published: March 2020

<p class="abstract"><strong>Background:</strong> Dermatoses which are not sexually transmitted are referred to as non-venereal dermatoses of external genitalia. Venereal and non-venereal dermatoses tend to be confused and it is associated with guilt feeling in affected patients. The aim of the study was to study the incidence of non-venereal lesions of the genitalia and to know the incidence of non-venereal lesions of the genitalia in association with dermatoses of other parts of the body.</p><p class="abstract"><strong>Methods:</strong> This was a cross-sectional, clinical and observational study in 250 female patients attending dermatovenereology OPD of Vydehi Hospital, Bengaluru with non-venereal genital lesions for a period of 18 months. cases having venereal diseases were excluded from the study.<strong></strong></p><p class="abstract"><strong>Results:</strong> The study included 250 female patients with non-venereal genital lesions. Twenty-five different types of non-venereal dermatoses were observed. Inflammatory disorders were most common accounting for 128 patients (51.2%). Among inflammatory disorders lichen simplex chronicus was seen most followed by lichen sclerosus. Candidiasis was found to be more common among infections and infestations. Vitiligo was the commonest pigmentary disorder seen in our study. Other cases noted include epidermal inclusion cyst, Bartholin cyst and fibroepithelial stromal polyp. Pre malignant conditions like Bowenoid papulosis and malignant lesions like squamous cell carcinoma are also seen.</p><p class="abstract"><strong>Conclusions:</strong> This study helps in understanding the importance of diagnosis of non-venereal dermatoses and to differentiate it from venereal causes.</p><p> </p>
Aktivitas Anticestoda In Vitro Metabolit Sekunder Daun Miana (Coleus blumei. Benth) terhadap Cacing Hymenolepis microstoma
Authors: Ridwan Yusuf , Satrija Fadjar , Handharyani Ekowati 
Published: March 2020

Penelitian ini untuk mengetahui golongan senyawa metabolit sekunder dari ekstrak daun miana (Coleus blumei. Benth) yang memiliki aktivitas anticestoda. Daun miana kering diekstraksi dengan metode yang sesuai untuk mendapatkan golongan senyawa metabolit sekunder alkaloid, flavonoid, tanin, dan steroid. Aktivitas anticestoda diukur dengan menghitung waktu kematian cacing Hymenolepis microstoma pada setiap golongan metabolit sekunder konsentrasi 10%. Hasil penelitian menunjukkan tanin memiliki aktivitas anticestoda yang kuat yang tidak berbeda dengan praziquatel sebagai anthelmintik komersial. Akivitas anticestoda flavonoid mirip dengan tanin akan tetapi aktivitasnya masih dibawah praziquatel. Alkaloid dan steroid memiliki aktivitas anticestoda yang lebih rendah dibandingkan dengan tanin dan flavonoid. Hasil penelitian ini membuktikan bahwa tanin sebagai senyawa metabolit sekunder yang paling aktif sebagai anticestoda dibandingkan flavonoid, alkaloid, dan steroid.
Physical Exercise Does Not Improve Colon Inflammation in Mice Induced Lambda Carrageenan
Authors: Qonitatillah Ana , Wigati Kristanti Wanito , Irawan Roedi 
Published: March 2020

Inflammatory Bowel Disease (IBD) is a well known reported gastrointestinal tract disease, which the prevalence continous to increase in Southeast Asia and other developing countries. Animal model have already been widely used for gut inflammation study. Lambda (λ) carrageenan is a chemical substance which commonly used to induced inflammation in IBD animal models. Physical exercise is a non-pharmacological therapy recommended for IBD. However, further study is needed to determine the effects of this therapy. This study was aimed to determine the physical exercise effect on colon inflammation in mice induced λ-carrageenan. Thirty-eight mice were divided into 2 groups. Control group (C) which induced with λ-carrageenan and treatment group (T) which induced by λ-carrageenan and treadmill exercise. The degree of colon inflammation was obtained by histopathological examination using Hematoxylin-Eosin (HE) staining and inflammation scoring system, which include inflammatory cell infiltration and intestinal architecture. All mice colon samples in both group were inflamed with varying degrees. The treatment group had a higher Inflammatory degree score than control group (p <0.05). Physical exercise does not improved the degree of colon inflammation in mice induced λ-carrageenan due to uncontrolled stress induction and water intake containing λ-carrageenan.
Modeling Of Conceptions Of Health: A New Tool In Health Education
Authors: JAVERLIAT Pascal , Pizon Frank , Gerbaud Laurent 
Published: March 2020

Abstract
BACKGROUND: Conceptions allow individuals to interpret reality and to act on it according to their own form of coherence and validity. If a student’s conception of health is not organized at the beginning of the course so as to accept that the three domains of the biopsychosocial concept act on an equal footing, it is possible that notions brought by the instructors, relating to a domain that the student considers less operational, are too far removed from his proximal zone of development. This article proposes to shed light on this issue through an analysis, leading to a modeling of students’ conception of health at the beginning their osteopathy studies.METHODS: A semi-directed questionnaire survey was conducted among osteopathy students during their first week of training. The contents of the verbatims were analyzed according to their blocks of meaning, categorized to bring out the different conceptions of health and then modeled using network visualization software to illustrate the links and the relative weight of the different items of conceptions.RESULTS: We identified 36 categories of conceptions of health. The modeling shows strong links between the psychological and biological domains, while the social domain is isolated.CONCLUSIONS: From an andragogical perspective, modeling conceptions of health can allow students to situate their life course in relation to health and instructors, in order to identify the components of health conceptions that they do not yet mobilize.
Flipped classroom is an approach of basic medical education for undergraduate students
Authors: Ren Xiang , Sui Lin lin , Guan You fei , Kong Ying , Liu Bo , Ding Yan fang , Ma Hai ying , Wang Xiu li , Kong Hui , Kong Li 
Published: March 2020

Abstract
Background: With the development of medical education, new teaching method, such as flipped teaching model, was paid attention in the process. However, the sharing elaborate courses was used in the flipped teaching model in basic medicine has not been well investigated. Therefore, the aim of this study was to evaluate students’ learning effect of the histology and biochemistry between traditional teaching methods and flipped teaching method in the basic medicine classes to provide a scientific evidence of a new model establishment in the medical students’ education.Methods: 180 medical students at the Dalian Medical University were enrolled in the process. Hyperthyroidism was chosen for the content of this study. The participants were randomly allocated into the common teaching model group with the traditional lecture-based manner or the flipped teaching model group with the sharing elaborate courses manner. There are paper test, online test and lab test for histology and embryology after learning. The scores including total score, paper test score, online test score and lab test score were compared in two differernt teaching model and students were required to complete the questionnaire to evaluate the teaching model.Results: The scores of total, online and lab test with flipped teaching model were higher than those in common teaching model in histology and embryology. Additionally, the scores of total, paper and lab test with flipped teaching model were higher than those in common teaching model in biochemistry.The feedback of all items in the questionnaires were much positive. More students agreed and accepted the flipped teaching method could help to improve their performance.Conclusions: The medical students at Dalian Medical University can benefit from the flipped classroom teaching model, and a flipped classroom teaching model may well serve some subject areas such as histology and biochemistry. The flipped classroom approach can serve as a potentially exciting new modality for teaching model in the future.
DNMT1 inhibits hepatocellular carcinoma cells by altering the epigenetic modification of Marveld1 gene
Authors: Zhang Song-Yan , Luo Qian , Wang Peng , Zhao Hao-Ran , Xu Hai-Tao , Zhao Liang , Qu Zhao-Wei , Sun Jian-Min , Zhang Yu-Bao , Zheng Biao 
Published: March 2020

Abstract
Background To study the effect of DNA methyltransferase (DNMT1) on Marveld1 in hepatocellular Carcinoma (HCC), and explore its role in the molecular mechanism of HCC occurrence and development. Methods The expression levels of Dnmt1 and Marveld1 genes were detected by RT-PCR in the intraoperative pathological specimens. The human hepatoma Bel7402 and SMMC7721 cell lines down-regulated by DNMT1 gene expression were constructed by SiRNA transfection. The methylation level of Marveld1 promoter region was detected after RT-PCR and West-blot verification. The expression of Marveld1 gene, cell proliferation, invasion and change of cell cycle were detected by RT-PCR and West-blot. The expressions of P53, CyclinD1, and P21 and P16 protein were detected by West-blot method. Results The expression of DNMT1 gene in human hepatocarcinoma tissues was higher than that in adjacent tissues. The methylation level of the Marveld1 gene promoter region in human hepatoma cell line down-regulated by DNMT1 gene expression was decreased, the expression level of Marveld1 gene was increased, and the proliferation and invasion ability of cells were weakened. The cell cycle was showed as G1-S phase arrest; P53 and P16 protein expressions were up-regulated, and CyclinD1 and P21 protein expressions were down-regulated. Conclusions Dnmt1 gene is highly expressed in human hepatocarcinoma tissues. Down-regulation of DNMT1 gene expression can decrease the methylation level of Marveld1 promoter region, up-regulate Marveld1 gene and human P53 and P16 proteins expressions, and down-regulate CyclinD1 and P21 proteins expressions, inhibit the proliferation and invasion of human hepatoma cells and can cause cell cycle G1-S arrest.
Dependence of the Ni0.4Zn0.6Fe2O4 spinel ferrite on frequency and temperature using impedance spectroscopy tool
Authors: Hajlaoui Mohsen Elain , Essebti Dhahri , Kamel Khirouni 
Published: March 2020

Abstract
Ni0.4Zn0.6Fe2O4 ferrites were prepared by the conventional solid-state method. The X-ray study allowed the identification of a single orthorhombic phase. The conductivity of the prepared sample was measured at different frequencies in the temperature range 340 K to 600 K. The obtained results are discussed in terms of charge carriers hopping. We showed that the dielectric permittivity is high at different temperatures and various frequencies. The impedance plane findings present semicircle arcs at various temperatures and an electrical equivalent circuit was determined. In addition, the obtained two relaxation times were established as a function of temperature.
An Improved Redundant Robotic Arm Parameter Identification Method
Authors: Liu Shi-Ping , Ma Ziyan , Chen Jin-Liang , Cao Jun-Feng , Fu Yan , Li Shi-Qi 
Published: March 2020

Abstract
In order to establish the dynamic model of redundant robotic arm more accurately, this paper studies the identification method of the dynamic parameters of redundant robotic arm. A method of introducing the theory of spins based on the Newton-Euler dynamics equations, and using the Coulomb + viscous friction model to further derive the minimum parameter set through linearization is proposed. The method of offline identification of current measurement is used to realize the parameter identification of the robotic arm. The excitation trajectory is designed in the basic form of Fourier series. The non-linear optimization function is used to optimize the coefficient of the trajectory with the minimum number of conditions in the observation matrix as the goal Therefore, the available joint trajectories are obtained, and the obtained parameter set has high accuracy and can meet the needs of actual control. Finally, the method was experimentally tested by actual robot Walker, which verified the accuracy and effectiveness of the proposed method.
Caracterização biométrica de Guabijuzeiros (Myrcianthes pungens (O. Berg) d. Legrand)
Authors: RODRIGUES Marlene Aparecida , GUERRA Divanilde , SEHN Talia Talita Sehn , BOHRER Robson Evaldo Gehlen Bohrer , SILVA Danni Maisa da 
Published: March 2020

O guabijuzeiro (Myrcianthes pungens (O. Berg) d. Legrand) se destaca pela produção de frutos para consumo in natura, porém estes são pouco conhecidos. O objetivo deste trabalho foi realizar a caracterização de 22 acessos de guabijuzeiros. As plantas, folhas e frutos foram avaliados quanto a sua morfologia. Como resultados observou-se que a média da circunferência do caule foi de 69,1 cm, a altura de 5,8 m, a largura das folhas de 2,61 cm e o comprimento de 6,1 cm. Nos frutos, obteve-se as médias de circunferência de 1,65 a 1,98 cm; massa total de 2,87 g; massa de polpa de 1,28 g, de casca de 0,76 g; de semente de 2,99 g; e o grau brix foi 12,53%; e massa seca de 3,41 g. A viabilidade média do pólen foi de 95,21%. Portanto, os acessos avaliados, podem ser propagados, pois possuem ótimas características morfológicas e qualidade de frutos.
Specificity of the development and characteristics of mixed damage to network structures of pipeline transportation systems
Authors: Tararychkin I. A. 
Published: March 2020

Pipeline transportation systems are used in various industries for the purpose of delivering various substances and materials to consumers. If, as the result of an accident development, a certain number of random linear elements (pipelines) consecutively fail, such scenario of events is called progressive damage. If several pipelines converging at a node fail simultaneously, such point element of the system is blocked. Progressive blocking of a certain set of nodes of a pipeline system in random order is called a progressive blocking. Simultaneous development within a system of progressive damage to linear elements and blocking of transportation nodes represents mixed damage. Mixed damage is a hazardous form of emergency, and its development causes fast degradation of a system’s transportation capabilities.TheAimof the paper is to study the characteristic properties and patterns of the progress of mixed damage affecting network structures of pipeline systems, as well as evaluating such systems’ capability to resist its development.Methods of research.The characteristics of network entities’ resilience to the development of mixed damage were identified by means of computer simulation. The nature of the effects to which a system is exposed was defined with a cyclogram, whose integer parameters indicate the alternation of the process of sequential damage of linear elements and nodes of a network structure.Results.It has been established that a correct comparison of the resilience of various network structures to mixed damage is only possible with regard to comparable facilities. For that purpose, the analyzed systems must have identical numbers of nodes, linear elements and end product consumers. Additionally, such systems must be exposed to effects with identical cyclograms. It is shown that the correlation of the resilience of comparable network structures does not depend on the specific type of mixed damage cyclogram, but is defined by the nature of the connections within a particular system.Conclusions.Mixed damage is a hazardous development scenario of an emergency situation that is associated with rapid degradation of the transportation capacity of pipeline systems. The ability of network structures of pipeline systems to resist mixed damage is evaluated based on indicators that are defined by means of simulation. A correct comparison of the resilience of various structures to mixed damage is only possible in case they are comparable. For that purpose, they must have identical numbers of nodes, linear elements and product consumers. Additionally, such systems must be exposed to damage procedures with identical cyclograms. The correlation of the resilience of network structures that comply with the comparability conditions does not depend on the adopted damage cyclogram, but is defined by the existing set of connections within a particular system.
Acute Myocardial Infarction in Systemic Mastocytosis: Case Report With Literature Review on the Role of Inflammatory Process in Acute Coronary Syndrome
Authors: Battisha Ayman , Sawalha Khalid , Madoukh Bader , Sheikh Omar , Doughem Karim , Al-Akchar Mohammad , Al-Sadawi Mohammed , Shaikh Shakil 
Published: March 2020

Background:
Systemic Mastocytosis (SM) is a disorder of excessive mast cell infiltration
in multiple organ tissues. Atherosclerosis is a major risk factor for developing acute coronary
syndrome. In addition to lipid accumulation in the arterial wall, inflammation plays an important
role in the pathogenesis of plaque rupture and activating the thrombosis cascade. The Mast cells
contribution to plaque destabilization has been well established in multiple animal and human studies.
In a recent study, SM has been proven to be associated with a higher incidence of acute coronary
syndrome even with lower plasma lipids levels. The study showed that 20% of patients with
SM had cardiovascular events compared to only 6% in the control group with adjustment to all cardiac
risk factors.

Case:
We presented a patient with no risk factors for heart disease other than old age and history of
SM who developed acute myocardial infarction.

Conclusion:
SM can be life-threatening and can result in ACS, anaphylactic reaction, syncope, or
cardiac arrest. Clinicians should have a high index of suspicion of acute coronary syndrome (ACS)
occurrence in the setting of inflammatory conditions, such as SM and KS, and vice versa, where
SM should be considered or ruled out in patients who suffer from anaphylaxis and cardiac arrest or
myocardial infarction.
Radioiodination of Pimonidazole as a Novel Theranostic Hypoxia Probe
Authors: Inci Ilknur Demir , Tekin Volkan , Kilcar Ayfer Yurt , Guldu Ozge Kozgus , Medine Emin Ilker , Karatay Kadriye Busra , Dervis Emine , Muftuler Fazilet Zumrut Biber 
Published: March 2020

Background:
Tumors are defined as abnormal tissue masses, and one of the most important
factors leading to the growth of these abnormal tissue masses is Vascular Endothelial Growth
Factor, which stimulates angiogenesis by releasing cells under hypoxic conditions. Hypoxia has a
vital role in cancer therapy, thus it is important to monitor hypoxia. The hypoxia marker Pimonidazole
(PIM) is a candidate biomarker of cancer aggressiveness.

Objective:
The study aimed to perform radioiodination of PIM with Iodine-131 (131I) to join a theranostic
approach. For this purpose, PIM was derived as PIM-TOS to be able to be radioiodinated.

Methods:
PIM was derived via a tosylation reaction. Derivatization product (PIM-TOS) was radioiodinated
by using iodogen method and was analyzed by High-Performance Liquid Chromatography
and Liquid chromatography-mass spectrometry. Thin layer radiochromatography was utilized
for its quality control studies.

Results:
PIM was derived successfully after the tosylation reaction. The radioiodination yield of
PIM-TOS was over 85%.

Conclusion:
In the current study, radioiodination potential of PIM with 131I, as a potential theranostic
hypoxia agent was investigated. Further experimental studies should be performed for developing
a novel hypoxia probe including theranostics approaches.
A Maximum Entropy Estimator for the Average Survival Time Differences between Two Groups
Authors: Manciu Marian , Hosseini Sorour , Guzman-Gonzalez Joscelyne 
Published: March 2020

Background:
Statistical methods commonly used in survival analysis typically provide the
probability that the difference between groups is due to chance, but do not offer a reliable estimate of
the average survival time difference between groups (the difference between median survival time is
usually reported).

Objective:
We suggest a Maximum-Entropy estimator for the average Survival Time Difference
(MESTD) between groups.

Methods:
The estimator is based on the extra survival time, which should be added to each member of
the group, to produce the maximum entropy of the result (resulting in the groups becoming most similar).
The estimator is calculated only from time to event data, does not necessarily assume hazard proportionality
and provides the magnitude of the clinical differences between the groups.

Results:
Monte Carlo simulations show that, even at low sample numbers (much lower than the ones
needed to prove that the two groups are statistically different), the MESTD estimator is a reliable predictor
of the clinical differences between the groups, and therefore can be used to estimate from (low
sample numbers) preliminary data whether or not the large sample number experiment is worth pursuing.

Conclusion:
By providing a reasonable estimate for the efficacy of a treatment (e.g., for cancer) even
for low sample data, it might provide useful insight in testing new methods for treatment (for example,
for quick testing of multiple combinations of cancer drugs).