bluuebunny/crossref_metadata_2025_split huggingface dataset:
Women's Perceptions on the Use of Video Technology in Early Labor: Being Able to See
Authors: Faucher Mary Ann , Kennedy Holly Powell 
Published: April 2020

IntroductionDelaying admission to the birth setting until active labor has commenced has known benefits. However, women and their partners often struggle to stay home in early labor. Research on telephone triage during early labor at home has illuminated significant disadvantages with this model of care, contributing to women feeling dissatisfied with the early birth experience. Research conducted with midwives on the potential benefits of using video technology suggests it might be a helpful strategy for early labor support. This study examined women's perspectives on the potential use of this technology.MethodsFocus groups and individual interviews were conducted with 23 English‐speaking women who experienced spontaneous labor within the last year. The recordings were transcribed verbatim. Content analysis was used to interpret women's perceptions.ResultsThe women identified potential advantages of video technology in early labor connected to the major theme of being able to see, which could enable closer human connections between the intrapartum care provider, the woman, and her partner, as well as better assessments of labor. This human connection was integral to enhancing empathy and building confidence. Concerns about using video calls during early labor at home focused on privacy issues and the need to practice beforehand. Concerns about privacy depended upon having a prior relationship with the intrapartum care provider and women being able to decide if they wanted to use the technology.DiscussionOne way of optimizing the experience of staying home in early labor and overall satisfaction with the birth experience may be with video technology, which could offer enhancements over traditional telephone triage.
Early onset demyelinating Charcot‐Marie‐Tooth disease caused by a novel in‐frame isoleucine deletion in peripheral myelin protein 2
Authors: Geroldi Alessandro , Prada Valeria , Veneri Francesca , Trevisan Lucia , Origone Paola , Grandis Marina , Schenone Angelo , Gemelli Chiara , Lanteri Paola , Fossa Paola , Mandich Paola , Bellone Emilia 
Published: April 2020

AbstractPeripheral myelin protein 2 (PMP2) is a small protein located on the cytoplasmic side of compact myelin, involved in the lipids transport and in the myelination process. In the last years few families affected with demyelinating Charcot‐Marie‐Tooth neuropathy (CMT1), caused by PMP2 mutations, have been identified. In this study we describe the first case of a PMP2 in‐frame deletion. PMP2 was analyzed by direct sequencing after exclusion of the most frequent CMT‐associated genes by using a next generation sequencing (NGS) genes panel. Sanger sequencing was used for family's segregation analysis. Molecular modeling analysis was used to evaluate the mutation impact on the protein structure. A novel PMP2: p.I50del has been identified in a child with early onset CMT1 and in three affected family members. All family members show an early onset demyelinating neuropathy without other distinguish features. Molecular modeling analysis and in silico evaluations do not suggest a strong impact on the overall protein structure, but a most likely altered protein function. This study suggests the importance to add PMP2 in CMT NGS genes panels or, at most, to test it after major CMT1 genes exclusion, due to the lack of diagnostic‐addressing additional features.
A longitudinal model of rejection sensitivity and internalizing symptoms: Testing emotion regulation deficits as a mechanism and outcome of symptoms
Authors: Gardner Alex A. , Zimmer‐Gembeck Melanie J. , Modecki Kathryn 
Published: April 2020

AbstractObjectiveIndividuals who experience heightened rejection sensitivity (RS) are at greater risk of increased internalizing symptoms over time. This is especially so for adolescents and young adults, as this is a time of many social transitions and an average increase in such symptoms. Yet, little longitudinal research has explored specific mechanisms that may help explain how RS lends itself to increased symptomology during adolescence and young adulthood. In this study, we tested the summative effect of emotion dysregulation, expressive suppression, and social avoidance (i.e., ER‐deficits) as mechanisms. Moreover, we estimated bidirectional temporal associations between ER‐deficits and symptoms.MethodParticipants included 402 adolescents and young adults aged 17 to 27 years (M = 19.9 years, 66% female) who completed two assessments over a 1‐year period.ResultsIn a path model, participants who reported more RS increased in anxious symptoms, and RS was indirectly associated with increased anxious and depressive symptoms via the three ER‐deficits. Additionally, cross‐lagged panel analyses showed that dysregulation and suppression predicted increased symptoms over time, while anxious symptoms predicted increased social avoidance over time.ConclusionThese findings expand understanding of the role of RS in young people's increasing internalizing symptoms, implicating ER‐deficits in these processes.
Discrimination between viable and dead Xanthomonas fragariae in strawberry using viability PCR
Authors: Immanuel Tracey , Taylor Robert , Keeling Stephanie , Brosnahan Cara , Alexander Brett 
Published: April 2020

AbstractXanthomonas fragariae is the causal agent of an important bacterial disease in strawberry production regions worldwide and a quarantine plant pathogen in many countries including New Zealand. Xanthomonas fragariae mainly infects the foliage of strawberry plants but can also infect the calyx tissue associated with strawberry fruit. Fresh strawberries are a high‐value internationally traded commodity that has a short shelf‐life. When making biosecurity decisions based on the finding of a quarantine organism such as X. fragariae by PCR, one of the major challenges is the inability to differentiate positive results originating from viable or dead cells. Viability PCR (vPCR) is a technique that selectively inhibits PCR amplification of DNA derived from dead cells through the use of a nucleic acid intercalating dye, for example, PEMAX™. A vPCR protocol has been optimized to enable rapid detection of viable X. fragariae in a tissue sample. PEMAX™ treatment resulted in complete inhibition of PCR amplification of 108–103 cfu/ml dead X. fragariae cells in strawberry host tissue. The most important parameters for optimization were the dilution of the sample, amplicon length and choice of nucleic acid intercalating dye. This study provides a rapid protocol to discriminate between viable and dead X. fragariae in strawberry in a phytosanitary environment. This test will help timely decisions to be made at the border on imported fresh strawberry consignments that test positive for X. fragariae.
Congenital protein C deficiency and thrombosis in a dog
Authors: Kelly Darren , Juvet Florence , Moore Gary 
Published: April 2020

AbstractCongenital protein C deficiency is an important cause of thrombosis in humans but is not described in dogs. A 4‐year‐old Hungarian Vizsla was presented for investigation of acute onset of ascites. Computed tomography of the chest and abdomen and echocardiography confirmed a large thrombus within the right ventricle. A cause for thrombosis was not initially identified. The clinical signs resolved rapidly and the dog was administered clopidogrel and discharged. Plasma protein C activity measured 2 and 6 weeks later was markedly lower than expected on both occasions. All known causes of acquired protein C deficiency were excluded, and the dog was diagnosed with a congenital protein C deficiency. After diagnosis, the administration of clopidogrel was stopped and administration of rivaroxaban was started. The dog remains well with no evidence of recurrent thrombosis with 6 months of follow‐up.
DNA topoisomerase 2 gene polymorphism in dermatophytes
Authors: Shamsizadeh Forough , Pchelin Ivan M. , Makimura Koichi , Alshahni Mohamed Mahdi , Satoh Kazuo , Katiraee Farzad , Ahmadi Bahram , Rezaei‐Matehhkolaei Ali 
Published: April 2020

SummaryBackgroundDermatophytes are a group of keratinophilic fungi of medical importance. Despite a relatively long history of molecular taxonomic studies, there is still a need for information on genetic polymorphism in wider variety of genomic loci.ObjectivesOur goal was to study partial DNA topoisomerase 2 gene (TOP2) polymorphism in dermatophytes.MethodsWe performed DNA sequencing of TOP2 in 26 dermatophyte species along with ribosomal internal transcribed spacer (ITS) sequencing.ResultsThe number of polymorphic sites in TOP2 data set was similar to that one in ITS data set. Nannizzia species formed paraphyletic group in TOP2 tree. Trichophyton simii was paraphyletic in concatenated TOP2‐ITS tree, one of its two clades contained solely Iranian isolates.ConclusionsOur results revealed several unresolved problems in the taxonomy of dermatophytes, including probable polyphyly of the genus Nannizzia and the species T simii.
Giving undergraduate nursing students international experiences: Issues and strategies
Authors: Turale Sue , Kunaviktikul Wipada , Mesukko Jutarat 
Published: April 2020

AbstractEducational institutions began international student placements or exchanges in earnest in the 1990s, with varying degrees of success. Although nursing education in a number of countries has embraced giving undergraduate students the opportunity of international experiences, this is still not the norm in many countries, nor within all nursing schools within a country. In our rapidly globalizing world, it is critical that students are given opportunities internationally to be better prepared for practice, to achieve a global mindset, and to take their place as future global citizens through international experiences. Evidence from the literature shows positive effects on nurses having had international experiences to broaden their horizons, examine other cultures, grow their cultural competence, share and gain knowledge with others, and build their capacity for future practice in an increasingly globalized world. Our focus here is on providing students with short‐term undergraduate exchanges or placements in international settings, and encouragement for nursing institutions to develop international partnerships that are sustainable. Some implications for nurse educators and other staff involved in international exchanges are also considered.
Two receptor‐like protein kinases, MUSTACHES and MUSTACHES‐LIKE, regulate lateral root development in Arabidopsis thaliana
Authors: Xun Qingqing , Wu Yunzhe , Li Hui , Chang Jinke , Ou Yang , He Kai , Gou Xiaoping , Tax Frans E. , Li Jia 
Published: April 2020

Summary

Receptor‐like protein kinases (RLKs) play key roles in regulating plant growth, development and stress adaptations. There are at least 610 RLKs (including receptor‐like cytoplasmic kinases) in Arabidopsis. The functions of the majority of RLKs have not yet been determined.

We previously generated promoter::GUS transgenic plants for all leucine‐rich repeat (LRR)‐RLKs in Arabidopsis and analyzed their expression patterns during various developmental stages. We found the expression of two LRR‐RLKs, MUSTACHES (MUS) and MUSTACHES‐LIKE (MUL), are overlapped in lateral root primordia. Independent mutants, mus‐3 mul‐1 and mus‐4 mul‐2, show a significantly decreased emerged lateral root phenotype.

Our analyses indicate that the defects of the double mutant occur mainly at stage I of lateral root development. Exogenous application of auxin can dramatically enhance the transcription of MUS, which is largely dependent on AUXIN RESPONSE FACTOR 7 (ARF7) and ARF19. MUS and MUL are inactive kinases in vitro but are phosphorylated in planta, possibly by an unknown kinase. The kinase activity of MUS is dispensable for its function in lateral root development. Many cell wall related genes are down regulated in mus‐3 mul‐1.

In conclusion, we identified MUS and MUL, two kinase‐inactive RLKs, in controlling the early development of lateral root primordia likely via regulating cell wall synthesis and remodeling.
Prognostic value of partial EMT‐related genes in head and neck squamous cell carcinoma by a bioinformatic analysis
Authors: Kisoda Satoru , Shao Wenhua , Fujiwara Natsumi , Mouri Yasuhiro , Tsunematsu Takaaki , Jin Shengjian , Arakaki Rieko , Ishimaru Naozumi , Kudo Yasusei 
Published: April 2020

AbstractObjectiveRecent studies have revealed that the ability of cancer cells to undergo intermediate state of epithelial‐to‐mesenchymal transition (EMT), partial EMT (p‐EMT), poses a higher metastatic risk rather than complete EMT. Here, we examined the prognostic value of p‐EMT‐related genes in head and neck squamous cell carcinoma (HNSCC) by bioinformatic approaches.Materials and methodsWe used RNA‐seq data of 519 primary HNSCC cases obtained from TCGA database. We compared the expression of p‐EMT‐related genes in HNSCC tissues with normal tissues. We evaluated the prognostic value of p‐EMT‐related genes in HNSCC cases by log‐rank test. We examined the expression of p‐EMT‐, EMT‐, and epithelial differentiation‐related genes by qPCR.ResultsAmong p‐EMT‐related genes that were highly expressed in HNSCC cases, high expression of SERPINE1, ITGA5, TGFBI, P4HA2, CDH13, and LAMC2 was significantly correlated with poor survival of HNSCC patients. By gene expression pattern, HNSCC cell lines were classified into three groups: epithelial phenotype, EMT phenotype, and p‐EMT phenotype.ConclusionsOur findings suggest that p‐EMT program may be involved in poor prognosis of HNSCC. SERPINE1, ITGA5, TGFBI, P4HA2, CDH13, and LAMC2 can be used for a prognostic marker. Moreover, HNSCC cells with p‐EMT phenotype can be a useful model for investigating a nature of p‐EMT.
Management of cementoblastoma in a 16‐year‐old boy: local block resection using PRF
Authors: Jamison A. , Kwok J. 
Published: April 2020

AbstractA 16‐year‐old boy attended with a hard buccal/lingual swelling associated with an erupted lower right first molar. The patient had been aware of its presence for at least 12 months. Cone‐beam CT investigation appeared consistent with a cementoblastoma associated with the roots of this tooth. Platelet‐rich fibrin and bone graft were used to recontour the surgical site following a block resection.
Upper extremity deep venous thrombosis and pulmonary embolism after transvenous lead replacement or upgrade procedures
Authors: Albertini Caio Marcos de Moraes , da Silva Katia Regina , Lima Marta Fernandes , Leal Filho Joaquim Maurício da Motta , Martinelli Filho Martino , Costa Roberto 
Published: April 2020

AbstractBackgroundVenous obstructions are frequent in patients with transvenous leads, although related clinical findings are rarely reported. After lead replacement or upgrade procedures, these lesions are even more frequent, but there is still no evidence to support this observation.AimTo investigate the incidence and possible risk factors for upper extremity deep venous thrombosis (UEDVT) and pulmonary embolism (PE) after lead replacement or upgrade procedures.MethodsProspective cohort carried out between April 2013 and July 2016. Preoperative evaluation included venous ultrasound and pulmonary angiotomography. Diagnostic exams were repeated postoperatively to detect the study outcomes. Multivariate logistic regression models were used to identify prognostic factors.ResultsAmong the 84 patients included, 44 (52.4%) were female and mean age was 59.3 ± 15.2 years. Lead malfunctioning (75.0%) was the main surgical procedure indication. Lead removal was performed in 44 (52.4%) cases. The rate of postoperative combined events was 32.6%, with 24 (28.6%) cases of UEDVT and six (7.1%) cases of PE. Clinical manifestations of deep venous thrombosis occurred in 10 (11.9%) patients. Independent prognostic factors for UEDVT were severe collateral circulation in the preoperative venography (odds ratio [OR] 4.7; 95% confidence interval [CI] 1.1–19.8; P = .037) and transvenous lead extraction (OR 27.4; 95% CI 5.8–128.8; P < .0001).ConclusionReoperations involving previously implanted transvenous leads present high rates of thromboembolic complications. Transvenous lead extraction had a significant impact on the development of UEDVT. These results show the need of further studies to evaluate the role of preventive strategies for this subgroup of patients.
The Evolving Role of High‐Frequency Spinal Cord Stimulation as Salvage Therapy in Neurostimulation
Authors: Ghosh Priyanka E. , Gill Jatinder S. , Simopoulos Thomas 
Published: April 2020

AbstractBackgroundHigh‐frequency 10‐kHz spinal cord stimulation (10‐kHz SCS) has shown promise in multicenter prospective trials for the management of chronic back and leg pain. Traditional spinal cord stimulation (t‐SCS) has a long history of effectiveness in chronic neuropathic syndromes but not uncommonly can fail to provide long‐term relief, leaving a significant group of patients with unsatisfactory outcomes. There is mounting evidence that 10‐kHz SCS may offer relief in this subset of patients.MethodsThe purpose of this retrospective analysis was to report a single‐institution long‐term experience of 10‐kHz SCS in patients who did not get adequate pain relief with prior t‐SCS devices. A temporary trial of 10‐kHz SCS was carried out for 7 days, and those experiencing an average of 50% reduction in pain intensity underwent implantation. Patients were classified as moderate responders if relief was 31% to 50% and excellent responders if pain relief exceeded 50%.ResultsThirty‐one patients who had experienced failed t‐SCS primarily from poor paresthesia coverage underwent a trial of 10‐kHz SCS and 29 underwent implantation. Twenty‐eight patients were available for analysis, with 57.1% experiencing 30% response and 46.4% experiencing excellent response at a median follow‐up of 21.2 (±8.4) months.ConclusionsThis small single‐institution study suggests that a significant proportion of patients with previously failed t‐SCS may achieve clinically meaningful and durable pain relief with 10‐kHz SCS.
Inter‐trial alpha power indicates mind wandering
Authors: Arnau Stefan , Löffler Christoph , Rummel Jan , Hagemann Dirk , Wascher Edmund , Schubert Anna‐Lena 
Published: April 2020

AbstractMind wandering during ongoing tasks can impede task performance and increase the risk of failure in the laboratory as well as in daily‐life tasks and work environments. Neurocognitive measures like the electroencephalography (EEG) offer the opportunity to assess mind wandering non‐invasively without interfering with the primary task. However, the literature on electrophysiological correlates of mind wandering is rather inconsistent. The present study aims toward clarifying this picture by breaking down the temporal dynamics of mind wandering encounters using a cluster‐based permutation approach. Participants performed a switching task during which mind wandering was occasionally assessed via thought probes applied after trial completion at random time points. In line with previous studies, response accuracy was reduced during mind wandering. Moreover, alpha power during the inter‐trial interval was a significantly increased on those trials on which participants reported that they had been mind‐wandering. This spatially widely distributed effect is theoretically well in line with recent findings linking an increased alpha power to an internally oriented state of attention. Measurements of alpha power may, therefore, be used to detect mind wandering online during critical tasks in traffic and industry in order to prevent failures.
Physically active individuals look for more: An eye‐tracking study of attentional bias
Authors: Cheval Boris , Miller Matthew W. , Orsholits Dan , Berry Tanya , Sander David , Boisgontier Matthieu P. 
Published: April 2020

AbstractAttentional capture by exercise‐related stimuli is important for the regulation of physical activity. Attentional processing underlying this capture has been investigated with indirect behavioral measures based on reaction times. To investigate more direct measures of visual spatial attention toward physical activity (vs. inactivity) stimuli, we used eye‐tracking and a visual dot probe task in 77 young adults with various level of physical activity. Reaction times to detect a dot appearing in the area previously occupied by a physical activity (vs. inactivity) stimulus were an indirect measure of attentional bias. The first picture gaze and viewing time were more direct measures of attentional orienting and attentional engagement, respectively. Pupil dilation was an indicator of arousal. Reaction times revealed a two‐way interaction between the location of the dot and participants’ usual level of physical activity. Only participants with a high level of physical activity more quickly detected a dot when it appeared in the area previously occupied by a physical activity stimulus. Eye‐tracking results showed greater odds of first gazing at physical activity stimuli and for a longer time, and a greater decrease in pupil size when viewing physical activity stimuli when usual level of physical activity was moderate or high, but not low. The variance explained in the outcomes ranged from 13.9% (pupil dilation) to 40% (reaction times). Overall, as hypothesized, compared to less physically active participants, participants who were more physically active demonstrated indirect (reaction times) and direct (first gaze, viewing time) evidence of a more pronounced attentional bias toward physical activity. Physical activity stimuli biased attention, with a pronounced effect when the level of physical activity was higher. These findings suggest that physical activity stimuli are relevant to the current concerns of moderately and highly active individuals.
Impact of obesity on dialysis and transplant and its management
Authors: Diwan Tayyab S. , Cuffy Madison C. , Linares‐Cervantes Ivan , Govil Amit 
Published: April 2020

AbstractObesity is increasing to unprecedented levels, including in the end‐stage kidney disease population, where upwards of 60% of kidney transplant patients are overweight or obese. Obesity poses additional challenges to the care of the dialysis patient, including difficulties in creating vascular access and inserting Tenckhoff catheters, higher rates of catheter malfunction and peritonitis, the need for longer and/or more frequent dialysis (or peritoneal dialysis [PD] exchanges) to achieve adequate clearance, increased metabolic complications particularly with PD, and obesity is a barrier to kidney transplantation. In this article, we review special considerations in performing PD, hemodialysis and transplant in the obese patient, as well as the evidence behind medical and surgical management of obesity in dialysis patients.