bluuebunny/crossref_metadata_2025_split huggingface dataset:
Epigenetic bases of grafting-induced vigour in eggplant
Authors: Cerruti Elisa , Gisbert Carmina , Drost Hajk-Georg , Valentino Danila , Portis Ezio , Barchi Lorenzo , Prohens Jaime , Lanteri Sergio , Comino Cinzia , Catoni Marco 
Published: November 2019

AbstractIn horticulture, grafting is a popular technique used to combine positive traits from two different plants. This is achieved by joining the plant top part (scion) onto a rootstock which contains the stem and roots. Despite its wide use, the biological mechanisms driving rootstock-induced alterations of the scion phenotype remain largely unknown. Given that epigenetics plays a crucial role during distance signalling in plants, we studied the genome-wide changes induced by DNA methylation in eggplant (Solanum melongena) plants grafted onto two interspecific rootstocks used to increase scion vigour. As a control, we compared any epigenetic effect found in such grafts to patterns detected in self-grafted plants. We found that vigour was associated with a specific change in scion gene expression and a genome-wide hypomethylation in CHH context. Interestingly, this hypomethylation correlated with the down-regulation of younger and potentially more active LTR retrotransposons (LTR-TEs), suggesting that graft-induced epigenetic modifications are associated to both physiological and molecular phenotypes in grafted plants. We propose that rootstocks can promote vigour by reducing DNA methylation in the scion genome, following similar principles found in some heterotic hybrids.
Vacuolar processing enzyme translocates to the vacuole through the autophagy pathway to induce programmed cell death
Authors: Teper-Bamnolker Paula , Danieli Raz , Peled-Zehavi Hadas , Belausov Eduard , Abu-Abied Mohamad , Avin-Wittenberg Tamar , Sadot Einat , Eshel Dani 
Published: November 2019

AbstractThe caspase-like vacuolar processing enzyme (VPE) is a key factor in programmed cell death (PCD) associated with plant stress responses. Growth medium lacking a carbon source and dark conditions caused punctate labeling of 35S::VPE1-GFP (StVPE1-GFP) in potato leaves. Carbon starvation of BY-2 cells induced higher VPE activity and PCD symptoms. Growing VPE-RNAi BY-2 cells without sucrose reduced VPE activity and prevented PCD symptoms. During extended exposure to carbon starvation, VPE expression and activity levels peaked, with a gradual increase in BY-2 cell death. Histological analysis of StVPE1-GFP in BY-2 cells showed that carbon starvation induces its translocation from the endoplasmic reticulum to the central vacuole, through tonoplast engulfment. Exposure of BY-2 culture to the autophagy inhibitor concanamycin A caused autophagic bodies accumulation in the cell vacuole. Such accumulation did not occur in the presence of 3-methyladenine, an inhibitor of early-stage autophagy. BY-2 cells constitutively expressing StATG8IL-RFP, an autophagosome marker, showed colocalization with the StVPE1-GFP protein in the cytoplasm and vacuole. RNAi silencing of the core autophagy component ATG4 in BY-2 cells reduced VPE activity and cell death. These results are the first to suggest that VPE translocates to the cell vacuole through the autophagy pathway, leading to PCD.One sentence summaryCarbon starvation induced programmed cell death by trafficking vacuolar processing enzyme through the autophagy pathway to the vacuole.
Stochastic model for the CheY-P molarity in the neighbourhood ofE. coliflagella motors
Authors: Fier G. , Hansmann D. , Buceta R. C. 
Published: November 2019

AbstractEscherichia coliserves as prototype for the study of peritrichous enteric bacteria that perform runs and tumbles alternately. Bacteria run forward as a result of the counterclockwise (CCW) rotation of their flagella bundle, which is located rearward, and perform tumbles when at least one of their flagella rotates clockwise (CW), moving away from the bundle. The flagella are hooked to molecular rotary motors of nanometric diameter able to make transitions between CCW and CW rotations that last up to one hundredth of a second. At the same time, flagella move or rotate the bacteria’s body microscopically during lapses that range between a tenth and ten seconds. We assume that the transitions between CCW and CW rotations occur solely by fluctuations of CheY-P molarity in the presence of two threshold values, and that a veto rule selects the run or tumble motions. We present Langevin equations for the CheY-P molarity in the vicinity of each molecular motor. This model allows to obtain the run- or tumble-time distribution as a linear combination of decreasing exponentials that is a function of the steady molarity of CheY-P in the neighbourhood of the molecular motor, which fits experimental data. In turn, if the internal signaling system is unstimulated, we show that the runtime distributions reach power-law behaviour, a characteristic of self-organized systems, in some time range and, afterwards, exponential cutoff. In addition, our model explains without any fitting parameters the ultrasensitivity of the flagella motors as a function of the steady state of CheY-P molarity. In addition, we show that the tumble bias for peritrichous bacterium has a similar sigmoid-shape to the CW bias, although shifted to lower concentrations when the flagella number increases. Thus, the increment in the flagella number allows lower operational values for each motor increasing amplification and robustness of the chemotatic signaling pathway.
Clonal reconstruction from time course genomic sequencing data
Authors: Ismail Wazim Mohammed , Tang Haixu 
Published: November 2019

AbstractBackgroundBacterial cells during many replication cycles accumulate spontaneous mutations, which result in the birth of novel clones. As a result of this clonal expansion, an evolving bacterial population has different clonal composition over time, as revealed in the long-term evolution experiments (LTEEs). Accurately inferring the haplotypes of novel clones as well as the clonal frequencies and the clonal evolutionary history in a bacterial population is useful for the characterization of the evolutionary pressure on multiple correlated mutations instead of that on individual mutations.ResultsIn this paper, we study the computational problem of reconstructing the haplotypes of bacterial clones from the variant allele frequencies observed from an evolving bacterial population at multiple time points. We formalize the problem using a maximum likelihood function, which is defined under the assumption that mutations occur spontaneously, and thus the likelihood of a mutation occurring in a specific clone is proportional to the frequency of the clone in the population when the mutation occurs. We develop a series of heuristic algorithms to address the maximum likelihood inference, and show through simulation experiments that the algorithms are fast and achieve near optimal accuracy that is practically plausible under the maximum likelihood framework. We also validate our method using experimental data obtained from a recent study on long-term evolution of Escherichia coli.ConclusionWe developed efficient algorithms to reconstruct the clonal evolution history from time course genomic sequencing data. Our algorithm can also incorporate clonal sequencing data to improve the reconstruction results when they are available. Based on the evaluation on both simulated and experimental sequencing data, our algorithms can achieve satisfactory results on the genome sequencing data from long-term evolution experiments.AvailabilityThe program (ClonalTREE) is available as open-source software on GitHub at https://github.com/COL-IU/ClonalTREE
Alfalfa leaf curl virus is transmitted by Aphis craccivora in a highly specific circulative manner
Authors: Ryckebusch F. , Sauvion N. , Granier M. , Roumagnac P. , Peterschmitt M. 
Published: November 2019

AbstractTwo members of the genus Capulavirus (Geminiviridae) are transmitted by aphids including Alfalfa leaf curl virus (ALCV) transmitted by Aphis craccivora. The capulavirus Euphorbia caput-medusae latent virus was shown here to be transmitted also by A. craccivora, using the population EuphorbiaSA. ALCV was transmissible by several A. craccivora populations including Robinia, but not EuphorbiaSA, reflecting a high transmission specificity. ALCV accumulation and localization were analyzed in whole insects, midguts, hemolymphs, and heads of aphids from both populations and from the non-vector species Acyrthosiphon pisum. A 6-day persistence was observed in A. craccivora populations but not in A. pisum. Vector and non-vector A. craccivora populations could be distinguished by contrasted virus accumulations and midgut intracellular localization. Results confirm that ALCV is transmitted according to a circulative-persistent mode, and are consistent with a gut barrier to the transmission of ALCV in A. pisum and a primary salivary gland barrier in A. craccivora.
Transcriptional reprogramming in fused cells is triggered by plasma-membrane diminution
Authors: Feliciano Daniel , Espinosa-Medina Isabel , Weigel Aubrey , Milano Kristin M. , Tang Zhonghua , Lee Tzumin , Kliman Harvey J. , Guller Seth M. , Ott Carolyn M. , Lippincott-Schwartz Jennifer 
Published: November 2019

SummaryDeveloping cells divide and differentiate, and in many tissues, such as bone, muscle, and placenta, cells fuse acquiring specialized functions. While it is known that fused-cells are differentiated, it is unclear what mechanisms trigger the programmatic-change, and whether cell-fusion alone drives differentiation. To address this, we employed a fusogen-mediated cell-fusion system involving undifferentiated cells in tissue culture. RNA-seq analysis revealed cell-fusion initiates a dramatic transcriptional change towards differentiation. Dissecting the mechanisms causing this reprogramming, we observed that after cell-fusion plasma-membrane surface area decreases through increased endocytosis. Consequently, glucose-transporters are internalized, and cytoplasmic-glucose and ATP transiently decrease. This low-energetic state activates AMPK, which inhibits YAP1, causing cell-cycle arrest. Impairing either endocytosis or AMPK prevents YAP1 inhibition and cell-cycle arrest after fusion. Together these data suggest that cell-fusion-induced differentiation does not need to rely on extrinsic-cues; rather the plasma-membrane diminishment forced by the geometric-transformations of cell-fusion cause transient cell-starvation that induces differentiation.
Combination antibiotic PMMA composites provide sustained broad-spectrum antimicrobial activity and allow for post-implantation refilling
Authors: Cyphert Erika L. , Lu Chao-yi , Marques Dylan W. , Learn Greg D. , von Recum Horst A. 
Published: November 2019

ABSTRACTAntibiotics are commonly added to poly(methyl methacrylate) (PMMA) by surgeons to locally treat infections such as in bone cement for joint replacement surgeries, but also as implantable antimicrobial “beads”. However, this strategy is of limited value in high risk patients where infections can be recurrent or chronic and otherwise hard to treat. Also when only one drug is incorporated and applied toward poly-microbial infections (multiple bacterial species), there is a high risk that bacteria can develop antibiotic resistance. To combat these limitations, we developed a combination-antibiotic PMMA composite system comprised of rifampicin-filled β-cyclodextrin (β-CD) microparticles added into PMMA filled with a second drug. Different formulations were evaluated through Zone-of-Inhibition, drug activity, antibiotic release and re-filling, as well as mechanical studies. Our combination-antibiotic PMMA composite system achieved up to an eight-fold increase in duration of antimicrobial activity in comparison to clinically used antibiotic-filled PMMA. Inclusion of CD microparticles also allowed for refilling of additional antibiotics after simulated implantation, resulting in additional windows of therapeutic efficacy. Mechanical testing showed that our tested formulations did have a small, but significant decrease in mechanical properties when compared to unmodified controls. While further studies are needed to determine whether the tested formulations are still suitable for load-bearing applications (e.g. bone cement), our composites are certainly amenable for a variety of non-load bearing applications (e.g. antimicrobial “beads” and temporary spacer in two-stage arthroscopic revisions).
Grey clustering model based on kernel and information field
Authors: Luo Dang , Huihui Zhang 
Published: November 2019

Purpose
The purpose of this paper is to propose a grey clustering model based on kernel and information field to deal with the situation in which both the observation values and the turning points of the whitenization weight function are interval grey numbers.

Design/methodology/approach
First, the “unreduced axiom of degree of greyness” was expanded to obtain the inference of “information field not-reducing”. Then, based on the theoretical basis of inference, the expression of whitenization weight function with interval grey number was provided. The grey clustering model and fuzzy clustering model were compared to analyse the relationship and difference between the two models. Finally, the paper model and the fuzzy clustering model were applied to the example analysis, and the interval grey number clustering model was established to analyse the influencing factors of regional drought disaster risk in Henan Province.

Findings
The example analysis results illustrate that although the two clustering methods have different theoretical basis, they are suitable for dealing with complex systems with uncertainty or grey characteristic, solving the problem of incomplete system information, which has certain feasibility and rationality. The clustering results of case study show that five influencing factors of regional drought disaster risk in Henan Province are divided into three classes, consistent with the actual situation, and they show the validity and practicability of the clustering model.

Originality/value
The paper proposes a new whitenization weight function with interval grey number that can transform interval grey number operations into real number operations. It not only simplifies the calculation steps, but it has a great significance for the “small data sets and poor information” grey system and has a universal applicability.
Could gamification improve visitors’ engagement?
Authors: la Cuadra Miguel Trigo-De , Vila-Lopez Natalia , Hernandez-Fernández Asunción 
Published: November 2019

Purpose
The experiences are the basis of the tourist sector and the creation of unique and unforgettable ones allows the differentiation from the competition. The purpose of this paper is to analyze the effects of the experience when visiting a zoo on our emotions and how they influence our (positive and/or negative) behaviors and to investigate whether an innovation (gamification programs) could be used to intensify the relations proposed.

Design/methodology/approach
The authors collected the experiences lived by 242 visitors in BIOPARC Valencia (a zoological park). Two subsamples were identified: 166 insatiable tourists who expressed that the gamification would complete their experience, and 76 conventional tourists who indicated that the current experience did not need any kind of improvement.

Findings
The results show that some of the proposed relationships are more tenuous among the insatiable visitors, defenders of gamification, which allows the authors to verify the possibilities offered by gamification.

Originality/value
First, although the impact of experiential modules on emotions and behaviors has already been investigated, as far as behavioral effects are concerned, the difference between positive behaviors (loyalty) and negative behaviors (complaints and claims) has not been addressed. Second, the relationship between both types of behaviors (positive and negative) in this sector has not been studied to date. Finally, although the literature recognizes the impact of technology and its importance as an instrument of experiential marketing, its empirical exploration remains uninvestigated. Indeed, to date, the willingness of consumers to adopt gamified strategies to improve their tourism experiences has not been investigated.
Dynamic behavior analysis of a rigid rotor supported by hydrostatic squeeze film dampers compensated with new electrorheological valve restrictors
Authors: Benadda Mohamed , Bouzidane Ahmed , Thomas Marc , Guilbault Raynald 
Published: November 2019

Purpose
This paper aims to propose a new hydrostatic squeeze film damper compensated with electrorheological valve restrictors to control the nonlinear dynamic behavior of a rigid rotor caused by high unbalance eccentricity ratio. To investigate the effect of electrorheological valve restrictors on the dynamic behavior of a rigid rotor, a nonlinear model is developed and presented.

Design/methodology/approach
The nonlinear results are compared with those obtained from a linear approach. The results show good agreement between the linear and nonlinear methods when the unbalanced force is small. The effects of unbalance eccentricity ratio and electric field on the vibration response and the bearing transmitted force are investigated using the nonlinear models.

Findings
The results of simulation performed that the harmonics generated by high unbalance eccentricities can be reduced by using hydrostatic squeeze film damper compensated with electrorheological valve restrictors.

Originality/value
The numerical results demonstrate that this type of smart hydrostatic squeeze film damper provides to hydrostatic designers a new bearing configuration suitable to control rotor vibrations and bearing transmitted forces, especially for high speed.
Understanding and implementing CRM initiatives in international markets
Authors: Roggeveen Anne L. , Beitelspacher Lauren 
Published: November 2019

PurposeDrawing from prior research, the purpose of this paper is to outline the benefits of cause-related marketing (CRM) campaigns for the sponsoring multinational organization and the non-profit, to examine the impact of firm–cause fit and how this fit forms and to describe consumer reactions to CRM campaigns. With this backdrop, the paper offers recommendations for how multinational corporations (MNCs) should undertake international CRM activities.Design/methodology/approachA comprehensive literature review, undertaken with a theoretical lens, helps reveal how CRM campaigns can be executed in international markets.FindingsWith accurate country market assessments, MNCs are better prepared to integrate with the local environment and deliver effective CRM strategies and value.Practical implicationsThis paper offers guidance for MNCs that are interested in expanding internationally in terms of how to develop an effective CRM in international markets. In addition, it explores relationship elements that are critical for creating a successful, synergistic relationship between the firm and the non-profit.Originality/valueWhen adopted effectively, CRM can be a valuable tool for firms as they expand internationally, by establishing relationships with local customers. This research suggests guidelines for multinationals to consider as they expand their presence globally and apply CRM in broader contexts.
Conventional banks and Fintechs: how digitization has transformed both models
Authors: Paulet Elisabeth , Mavoori Hareesh 
Published: November 2019

Purpose
The digital revolution has substantially changed the business environment. Most banks have acknowledged the importance of new technologies to improve performance and client satisfaction. The development of these innovations has led to the entrance of the so-called Fintechs. This paper aims to evaluate the impact of these transformations on the performance of financial institutions and on their business model.

Design/methodology/approach
The authors use data envelopment analysis and Malmquist total productivity indices to measure financial institutions’ efficiency and their influence on strategy.

Findings
The main finding is that clients are more than ever at the core of banking strategy. The irrelevance of distance in basic banking transactions has reduced expenses and contributed to increasing revenues for all financial institutions. Banks will have a card to play in the advice they can bring to their clients.

Practical implications
This research could be of interest for financial managers who wish to re-examine their current business practices and imagine their business model for the future.

Originality/value
The contribution will be to further define the correlation between the provision of electronic banking services and its performance by including diversified institutions (conventional banks, Fintechs, Gafas) in the sample from multiple geographic zones to identify differences as regards their efficiency and business practices.
Stock market activity and Google Trends: the case of a developing economy
Authors: Bui Vinh Xuan , Nguyen Hang Thu 
Published: November 2019

PurposeThe purpose of this paper is to investigate the impacts of investor attention on stock market activity.Design/methodology/approachThe authors employed the Google Search Volume (GSV) Index, a direct and non-traditional proxy for investor attention.FindingsThe results indicate a strong correlation between GSV and trading volume – a traditional measure of attention – proving the new measure’s reliability. In addition, market-wide attention increases both stock illiquidity and volatility, whereas company-level attention shows mixed results, driving illiquidity and volatility in both directions.Originality/valueTo the best of the authors’ knowledge, Nguyen and Pham’s (2018) study has been the only previous study identifying investor attention in Vietnam by using GSV as a proxy and examining the impacts of broad search terms about the macroeconomy on the stock market as a whole – on stock indices’ movements. The paper will contribute to this by quantifying GSV impacts on each stock individually.
Effects of government policy, quality of human resources and professional institutions on workforce competitiveness using welding technology as mediating variable
Authors: Agusman Aris Afandi , Maupa Haris , Muis Mahlia , Idrus Tabba Muhammad 
Published: November 2019

Purpose
This paper aims to examine and analyze the effects of government policy, quality of human resources and professional institutions on workforce competitiveness using welding technology variable as a mediating variable.

Design/methodology/approach
This study used quantitative research by using partial least square – structural equation modeling (PLS-SEM) to analyze the collected data.

Findings
Based on the results of the analysis, it was noted that there was a significant influence between government policy, quality of human resources and professional institutions on welding technology. The coefficients are characterized by a positive direct relationship, which means that the higher the quality of government policy and human resources professionals variables, the higher the value of the institute of welding technology.

Social implications
This study recommends that government should create policies that have benefits to competitiveness of Indonesian workforce. Implications from this study support government to use the model to determine and initiate policies in the field of welding as well as establish clear and standardized operating standards and recruitment process (government apparatus) that can accommodate the competitiveness of welding workers in Indonesia.

Originality/value
The originality of this paper is that the participatory approach was adopted in this study using PLS-SEM. In addition, this study was one of the first studies to carry out research at the BNSP office, BLK-Bandung-Jakarta, Makassar, B4T and dismiss the Ministry of Manpower and the Ministry of Industry in Jakarta, Indonesia, where there was no research in this location. Previous studies conducted research in various case studies.