bluuebunny/crossref_metadata_2025_split huggingface dataset:
Developing a Lay First Responder Program in Chad: A 12-Month Follow-Up Evaluation of a Rural Prehospital Emergency Care Program
Authors: Hancock Canaan J. , Delaney Peter G. , Eisner Zachary J. , Kroner Eric , Mahamet-Nuur Issa , Scott John W. , Raghavendran Krishnan 
Published: July 2020

AbstractIntroduction:The World Health Organization (WHO; Geneva, Switzerland) recommends lay first responder (LFR) programs as a first step toward establishing formal Emergency Medical Services (EMS) in low- and middle-income countries (LMICs) to address injury. There is a scarcity of research investigating LFR program development in predominantly rural settings of LMICs.Study Objective:A pilot LFR program was launched and assessed over 12 months to investigate the feasibility of leveraging pre-existing transportation providers to scale up prehospital emergency care in rural, low-resource settings of LMICs.Methods:An LFR program was established in rural Chad to evaluate curriculum efficacy, using a validated 15-question pre-/post-test to measure participant knowledge improvement. Pre-/post-test score distributions were compared using a Wilcoxon Signed-Rank test. For test evaluation, each pre-test question was mapped to its corresponding post-test analog and compared using McNemar’s Chi-Squared Test to examine knowledge acquisition on a by-question basis. Longitudinal prehospital care was evaluated with incident reports, while program cost was tracked using a one-way sensitivity analysis. Qualitative follow-up surveys and semi-interviews were conducted at 12 months, with initial participants and randomly sampled motorcycle taxi drivers, and used a constructivist grounded theory approach to understand the factors motivating continued voluntary participation to inform future program continuity. The consolidated criteria for reporting qualitative research (COREQ) checklist was used to guide design, analysis, and reporting the qualitative results.Results:A total of 108 motorcycle taxi participants demonstrated significant knowledge improvement (P <.001) across three of four curricular categories: scene safety, airway and breathing, and bleeding control. Lay first responders treated 71 patients over six months, encountering five deaths, and provided patient transport in 82% of encounters. Lay first responders reported an average confidence score of 8.53/10 (n = 38). In qualitative follow-up surveys and semi-structured interviews, the ability to care for the injured, new knowledge/skills, and the resultant gain in social status and customer acquisition motivated continued involvement as LFRs. Ninety-six percent of untrained, randomly sampled motorcycle taxi drivers reported they would be willing to pay to participate in future training courses.Conclusion:Lay first responder programs appear feasible and cost-effective in rural LMIC settings. Participants demonstrate significant knowledge acquisition, and after 12 months of providing emergency care, report sustained voluntary participation due to social and financial benefits, suggesting sustainability and scalability of LFR programs in low-resource settings.
Modified Mediterranean diet score adapted to a southern Mediterranean population and its relation to overweight and obesity risk
Authors: El Kinany Khaoula , Mint Sidi Deoula Meimouna , Hatime Zineb , Boudouaya Hanae Abir , Atassi Mariam , El Asri Achraf , Benslimane Abdelilah , Nejjari Chakib , Ibrahimi Sidi Adil , Lagiou Pagona , El Rhazi Karima 
Published: July 2020

AbstractObjectives:The current study aimed to develop a modified Mediterranean diet (MMD) score adjusted to the southern Mediterranean countries’ cultural specificities and to evaluate associations between adherence to this modified score and overweight/obesity risk in Moroccan adults.Design:Population-based cross-sectional study.Setting:Rural and urban areas of the five greatest provinces of Morocco.Participants:In total, 1516 participants were recruited between September 2009 and February 2017. Dietary assessment was obtained using a validated Moroccan FFQ. We constructed a MMD score focusing on twelve components. The MMD score ranged from 0 (no adherence to the traditional southern Mediterranean diet (MD)) to 12 (maximal adherence) and was categorised as low (scores 0–4), moderate (scores 5–7) and high (scores 8–12).Results:Among the whole population, 754 (50·5 %) were women and 738 (49·5 %) were men, and the mean age was about 55·60 ± 13·70. In total, 58 % of participants were moderately active. Regarding educational level, 50·7 % were illiterate. The prevalence of overweight and obesity was 43·3 and 8·6 %, respectively. In multivariate analyses, close adherence to MMD (scores 8–12) was associated with reduced overweight/obesity risk (OR 0·61, 95 % CI 0·44, 0·84).Conclusion:The prevalence of overweight and obesity was very high among Moroccan adults. Adherence to the traditional southern MD may help prevent overweight and obesity.
Effect of Dust Load on the Cloud Top Ice‐Water Partitioning Over Northern Middle to High Latitudes With CALIPSO Products
Authors: Kawamoto Kazuaki , Yamauchi Akira , Suzuki Kentaroh , Okamoto Hajime , Li Jiming 
Published: July 2020

AbstractWe quantified effects of dust load on the cloud top ice cloud fraction (ICF) in terms of the dust extinction coefficient (σext). We analyzed 3‐year data sets obtained from an active satellite sensor over middle to high latitudes in the northern hemisphere for temperatures (T) between 230 and 273 K and σext values between 0.005 and 0.145 km−1. At about 250 K, ICF changed by about 30% in response to the above range of σext, whereas at extreme T values, ICF was relatively insensitive to σext. Thus, we concluded that ICF was primarily determined by T, with substantial influence of σext at about 250 K, likely due to increased opportunities for freezing as σext increases. Sensitivity of ICF was the lowest both at the largest σext and lowest T and at the smallest σext and highest T, while it was the highest at about 0.03 km−1 of σext and about 250 K.
CO2‐Brine Substitution Effects on Ultrasonic Wave Propagation Through Sandstone With Oblique Fractures
Authors: Falcon‐Suarez Ismael Himar , Papageorgiou Giorgos , Jin Zhaoyu , Muñoz‐Ibáñez Andrea , Chapman Mark , Best Angus I. 
Published: July 2020

AbstractSeismic monitoring of injected CO2 plumes in fractured storage reservoirs relies on accurate knowledge of the physical mechanisms governing elastic wave propagation, as described by appropriate, validated rock physics models. We measured laboratory ultrasonic velocity and attenuation of P and S waves, and electrical resistivity, of a synthetic fractured sandstone with obliquely aligned (penny‐shaped) fractures, undergoing a brine‐CO2 flow‐through test at simulated reservoir pressure and temperature. Our results show systematic differences in the dependence of velocity and attenuation on fluid saturation between imbibition and drainage episodes, which we attribute to uniform and patchy fluid distributions, respectively, and the relative permeability of CO2 and brine in the rock. This behavior is consistent with predictions from a multifluid rock physics model, facilitating the identification of the dispersive mechanisms associated with wave‐induced fluid flow in fractured systems at seismic scales.
Exceptional Tropical Cyclone Kenneth in the Far Northern Mozambique Channel and Ocean Eddy Influences
Authors: Mawren D. , Hermes J. , Reason C. J. C. 
Published: July 2020

AbstractDuring summer 2018/2019, Mozambique was devastated by Idai and Kenneth, the first time two Intense Status tropical cyclones have ever made landfall here. The season broke numerous records for the Southwest Indian Ocean (the greatest deaths, the largest damage, and numbers of Intense Status cyclones and tropical storms). Kenneth, which is the strongest cyclone ever to make landfall in Mozambique, did so unusually late in the season, had an unusual due westward track and struck the coast far further north than previously, is examined. Changes in Kenneth's strength (21–25 April 2019) across the extreme northern Mozambique Channel are shown to be related to two cyclonic/anticyclonic eddy pairs in the ocean. These eddies led to changes in SST, enthalpy fluxes, tropical cyclone heat potential, and upper ocean heat content that influenced its rapid strengthening from moderate tropical storm to Very Intense tropical cyclone status and then back to Intense Status just before landfall.
High Sensitivity of Arctic Black Carbon Radiative Effects to Subgrid Vertical Velocity in Aerosol Activation
Authors: Matsui Hitoshi , Moteki Nobuhiro 
Published: July 2020

AbstractThe representation of aerosol activation into cloud droplets in climate models is important for accurate understanding of aerosol radiative impacts on the Arctic climate, but it remains highly uncertain. Here we show that the uncertainty range of subgrid vertical velocity (SVV) and maximum supersaturation (SSmax) in aerosol activation produces fourfold to fivefold differences in the direct radiative effect of black carbon (BC) in the Arctic (0.091–0.40 W m−2) because SVV and SSmax determine the activated fraction and wet removal efficiency of aerosols. Aerosols are particularly sensitive to SVV in remote regions but not near their sources because many aerosols near sources are not yet influenced by wet removal processes. Our results demonstrate that SVV treatment is a major source of uncertainty in Arctic aerosol simulations and may be key for reducing the large discrepancies among global models in estimates of BC and its radiative effects in the Arctic.
Hydrologic Implications of Wind Farm Effect on Radar‐Rainfall Observations
Authors: Ghimire Ganesh R. , Krajewski Witold F. 
Published: July 2020

AbstractDespite efforts to detect and mitigate wind farm clutter in weather radar observations of rainfall, these signatures propagate to quantitative precipitation estimates. In this study, the authors investigate the hydrologic impact of wind farm clutter in the Multi‐Radar Multi‐Sensor rainfall products. The study uses the probability of detection method to identify wind farm clutter in data from Iowa for the years 2016 and 2017. Using the physically based distributed hydrologic model called the Hillslope‐Link Model, the authors show that streamflow (flood) prediction errors are generally significant at smaller basin communities where wind farms occupy a large portion of the upstream basins. These errors due to wind farm clutter show systematic decrease with the basin scales. The results from this study have implications for real‐time streamflow forecasts provided automatically by the National Water Model at the National Oceanic and Atmospheric Administration, particularly at small riverine communities.
Impaired AKT signaling and lung tumorigenesis by PIERCE1 ablation in KRAS-mutant non-small cell lung cancer
Authors: Roh Jae-il , Lee Jaehoon , Sung Young-Hoon , Oh Jahyun , Hyeon Do Young , Kim Yujin , Lee Seungeon , Devkota Sushil , Kim Hye Jeong , Park Bomin , Nam Taewook , Song Yaechan , Kim Yonghwan , Hwang Daehee , Lee Han-Woong 
Published: July 2020

AbstractKRAS-mutant non-small cell lung cancer (NSCLC) is a major lung cancer subtype that leads to many cancer-related deaths worldwide. Although numerous studies on KRAS-mutant type NSCLC have been conducted, new oncogenic or tumor suppressive genes need to be detected because a large proportion of NSCLC patients does not respond to currently used therapeutics. Here, we show the tumor-promoting function of a cell cycle-related protein, PIERCE1, in KRAS-mutant NSCLC. Mechanistically, PIERCE1 depletion inhibits cell growth and AKT phosphorylation (pAKT) at S473, which is particularly observed in KRAS-mutant lung cancers. Analyses of AKT-related genes using microarray, immunoblotting, and real-time quantitative PCR indicated that PIERCE1 negatively regulates the gene expression of the AKT suppressor, TRIB3, through the CHOP pathway, which is a key regulatory pathway for TRIB3 expression. Similarly, in vivo analyses of PIERCE1 depletion in the KRAS mutation-related lung cancer mouse models revealed the suppressive effect of PIERCE1 knockout in urethane- and KRASG12D-induced lung tumorigenesis with decreased pAKT levels observed in the tumors. Tissue microarrays of human lung cancers indicated the expression of PIERCE1 in 83% of lung cancers and its correlation with pAKT expression. Thus, we illustrate how PIERCE1 depletion may serve as a therapeutic strategy against KRAS-mutant NSCLC and propose the clinical benefit of PIERCE1.
The identification of nuclear αvβ3 integrin in ovarian cancer: non-paradigmal localization with cancer promoting actions
Authors: Seraya-Bareket Chen , Weisz Avivit , Shinderman-Maman Elena , Teper-Roth Sharon , Stamler Dina , Arbib Nissim , Kadan Yfat , Fishman Ami , Kidron Debora , Edelstein Evgeny , Ellis Martin , Ashur-Fabian Osnat 
Published: July 2020

AbstractNuclear translocation of transmembrane proteins was reported in high-grade serous ovarian cancer (HGSOC), a highly aggressive gynecological malignancy. Although the membrane receptor αvβ3 integrin is amply expressed in HGSOC and involved in disease progression, its nuclear localization was never demonstrated. Nuclear αvβ3 was explored in HGSOC cells (OVCAR3, KURAMOCHI, and JHOS4), nuclear localization signal (NLS) modified β3 OVCAR3, Chinese hamster ovaries (CHO-K1) and human embryonic kidney (HEK293) before/after transfections with β3/β1 integrins. We used the ImageStream technology, Western blots (WB), co immunoprecipitations (Co-IP), confocal immunofluorescence (IF) microscopy, flow cytometry for cell counts and cell cycle, wound healing assays and proteomics analyses. Fresh/archived tumor tissues were collected from nine HGSOC patients and normal ovarian and fallopian tube (FT) tissues from eight nononcological patients and assessed for nuclear αvβ3 by WB, confocal IF microscopy and immunohistochemistry (IHC). We identified nuclear αvβ3 in HGSOC cells and tissues, but not in normal ovaries and FTs. The nuclear integrin was Tyr 759 phosphorylated and functionally active. Nuclear αvβ3 enriched OVCAR3 cells demonstrated induced proliferation and oncogenic signaling, intact colony formation ability and inhibited migration. Proteomics analyses revealed a network of nuclear αvβ3-bound proteins, many of which with key cancer-relevant activities. Identification of atypical nuclear localization of the αvβ3 integrin in HGSOC challenges the prevalent conception that the setting in which this receptor exerts its pleiotropic actions is exclusively at the cell membrane. This discovery proposes αvβ3 moonlighting functions and may improve our understanding of the molecular basis of ovarian cancer pathogenesis.
Targeted therapies in gynecological cancers: a comprehensive review of clinical evidence
Authors: Wang Qiao , Peng Hongling , Qi Xiaorong , Wu Min , Zhao Xia 
Published: July 2020

AbstractAdvanced and recurrent gynecological cancers are associated with poor prognosis and lack of effective treatment. The developments of the molecular mechanisms on cancer progression provide insight into novel targeted therapies, which are emerging as groundbreaking and promising cancer treatment strategies. In gynecologic malignancies, potential therapeutic targeted agents include antiangiogenic agents, poly (ADP-ribose) polymerase (PARP) inhibitors, tumor-intrinsic signaling pathway inhibitors, selective estrogen receptor downregulators, and immune checkpoint inhibitors. In this article, we provide a comprehensive review of the clinical evidence of targeted agents in gynecological cancers and discuss the future implication.
New insights into the evasion of host innate immunity by Mycobacterium tuberculosis
Authors: Chai Qiyao , Wang Lin , Liu Cui Hua , Ge Baoxue 
Published: July 2020

AbstractMycobacterium tuberculosis (Mtb) is an extremely successful intracellular pathogen that causes tuberculosis (TB), which remains the leading infectious cause of human death. The early interactions between Mtb and the host innate immune system largely determine the establishment of TB infection and disease development. Upon infection, host cells detect Mtb through a set of innate immune receptors and launch a range of cellular innate immune events. However, these innate defense mechanisms are extensively modulated by Mtb to avoid host immune clearance. In this review, we describe the emerging role of cytosolic nucleic acid-sensing pathways at the host–Mtb interface and summarize recently revealed mechanisms by which Mtb circumvents host cellular innate immune strategies such as membrane trafficking and integrity, cell death and autophagy. In addition, we discuss the newly elucidated strategies by which Mtb manipulates the host molecular regulatory machinery of innate immunity, including the intranuclear regulatory machinery, the ubiquitin system, and cellular intrinsic immune components. A better understanding of innate immune evasion mechanisms adopted by Mtb will provide new insights into TB pathogenesis and contribute to the development of more effective TB vaccines and therapies.
Index and biological spectrum of human DNase I hypersensitive sites
Authors: Meuleman Wouter , Muratov Alexander , Rynes Eric , Halow Jessica , Lee Kristen , Bates Daniel , Diegel Morgan , Dunn Douglas , Neri Fidencio , Teodosiadis Athanasios , Reynolds Alex , Haugen Eric , Nelson Jemma , Johnson Audra , Frerker Mark , Buckley Michael , Sandstrom Richard , Vierstra Jeff , Kaul Rajinder , Stamatoyannopoulos John 
Published: July 2020

AbstractDNase I hypersensitive sites (DHSs) are generic markers of regulatory DNA1–5 and contain genetic variations associated with diseases and phenotypic traits6–8. We created high-resolution maps of DHSs from 733 human biosamples encompassing 438 cell and tissue types and states, and integrated these to delineate and numerically index approximately 3.6 million DHSs within the human genome sequence, providing a common coordinate system for regulatory DNA. Here we show that these maps highly resolve the cis-regulatory compartment of the human genome, which encodes unexpectedly diverse cell- and tissue-selective regulatory programs at very high density. These programs can be captured comprehensively by a simple vocabulary that enables the assignment to each DHS of a regulatory barcode that encapsulates its tissue manifestations, and global annotation of protein-coding and non-coding RNA genes in a manner orthogonal to gene expression. Finally, we show that sharply resolved DHSs markedly enhance the genetic association and heritability signals of diseases and traits. Rather than being confined to a small number of distal elements or promoters, we find that genetic signals converge on congruently regulated sets of DHSs that decorate entire gene bodies. Together, our results create a universal, extensible coordinate system and vocabulary for human regulatory DNA marked by DHSs, and provide a new global perspective on the architecture of human gene regulation.
Detrimental effects of hypercortisolism on brain structure and related risk factors
Authors: Chen Yaxi , Zhang Junhuai , Tan Huiwen , Li Jiaqi , Yu Yerong 
Published: July 2020

AbstractBrain structural abnormalities are often observed on magnetic resonance imaging (MRI) scans of Cushing's syndrome patients, but the pathogenesis is not fully understood. To understand the relationship between brain structural abnormalities and potential risk factors in active Cushing's disease (CD) patients, a total of 101 treatment-naïve CD patients and 95 sex-, age- and education matched controls with non-functioning adenomas (NFA) underwent clinical evaluation and MRI investigation, and the relative risk factors were analyzed. 14 patients in sustained remission after transsphenoidal surgery were followed. Compared with the NFA subjects, the patients with CD had more cortical (P < 0.01) and subcortical atrophy (P < 0.01) and a higher prevalence of white matter hyperintensity (WMH) (P < 0.01). WMH severity in CD patients positively correlated with age (r = 0.532, P = 0.000), disease course (r = 0.257, P = 0.009), postprandial glucose (r = 0.278, P = 0.005), frequency of left ventricular hypertrophy (r = 0.398, P = 0.001) and hypothyroidism (r = 0.246, P = 0.014). The markers of cortical and subcortical atrophy (sylvian fissure ratio, bifrontal ratio, bicaudate ratio and third ventricle width) were positively associated with the progression of WMH in the CD patients. In the follow-up of 14 patients with CD, brain atrophy and WMH was partially reversible after correction of hypercortisolism. In conclusions, brain atrophy and WMH were more likely to appear in CD patients and were possibly partially reversible following correction of hypercortisolism.
Individual-based population genomics reveal different drivers of adaptation in sympatric fish
Authors: Torrado Héctor , Carreras Carlos , Raventos Núria , Macpherson Enrique , Pascual Marta 
Published: July 2020

AbstractConnectivity and local adaptation are two contrasting evolutionary forces highly influencing population structure. To evaluate the impact of early-life traits and environmental conditions on genetic structuring and adaptation, we studied two sympatric fish species in the Western Mediterranean Sea: Symphodus tinca and S. ocellatus. We followed an individual-based approach and measured early-life history traits from otolith readings, gathered information on environmental variables and obtained genome-wide markers from genotyping-by-sequencing (GBS). The two species presented contrasting population structure across the same geographic gradient, with high and significant population differentiation in S. ocellatus, mostly determined by oceanographic fronts, and low differentiation and no front effect in S. tinca. Despite their different levels of genetic differentiation, we identified in both species candidate regions for local adaptation by combining outlier analysis with environmental and phenotypic association analyses. Most candidate loci were associated to temperature and productivity in S. ocellatus and to temperature and turbulence in S. tinca suggesting that different drivers may determine genomic diversity and differentiation in each species. Globally, our study highlights that individual-based approach combining genomic, environmental and phenotypic information is key to identify signals of selection and the processes mediating them.