bluuebunny/crossref_metadata_2025_split huggingface dataset:
Extracellular Vesicle–Derived miR-124 Resolves Radiation-Induced Brain Injury
Authors: Leavitt Ron J. , Acharya Munjal M. , Baulch Janet E. , Limoli Charles L. 
Published: August 2020

Abstract

Radiation-induced cognitive dysfunction (RICD) is a progressive and debilitating health issue facing patients following cranial radiotherapy to control central nervous system cancers. There has been some success treating RICD in rodents using human neural stem cell (hNSC) transplantation, but the procedure is invasive, requires immunosuppression, and could cause other complications such as teratoma formation. Extracellular vesicles (EV) are nanoscale membrane-bound structures that contain biological contents including mRNA, miRNA, proteins, and lipids that can be readily isolated from conditioned culture media. It has been previously shown that hNSC-derived EV resolves RICD following cranial irradiation using an immunocompromised rodent model. Here, we use immunocompetent wild-type mice to show that hNSC-derived EV treatment administered either intravenously via retro-orbital vein injection or via intracranial transplantation can ameliorate cognitive deficits following 9 Gy head-only irradiation. Cognitive function assessed on the novel place recognition, novel object recognition, and temporal order tasks was not only improved at early (5 weeks) but also at delayed (6 months) postirradiation times with just a single EV treatment. Improved behavioral outcomes were also associated with reduced neuroinflammation as measured by a reduction in activated microglia. To identify the mechanism of action, analysis of EV cargo implicated miRNA (miR-124) as a potential candidate in the mitigation of RICD. Furthermore, viral vector–mediated overexpression of miR-124 in the irradiated brain ameliorated RICD and reduced microglial activation. Our findings demonstrate for the first time that systemic administration of hNSC-derived EV abrogates RICD and neuroinflammation in cranially irradiated wild-type rodents through a mechanism involving miR-124.

Significance:
Radiation-induced neurocognitive decrements in immunocompetent mice can be resolved by systemic delivery of hNSC-derived EVs involving a mechanism dependent on expression of miR-124.
Dynamics of Cardiac Neutrophil Diversity in Murine Myocardial Infarction
Authors: Vafadarnejad Ehsan , Rizzo Giuseppe , Krampert Laura , Arampatzi Panagiota , Arias-Loza Anahi-Paula , Nazzal Yara , Rizakou Anna , Knochenhauer Tim , Bandi Sourish Reddy , Nugroho Vallery Audy , Schulz Dirk J.J , Roesch Melanie , Alayrac Paul , Vilar Jose , Silvestre Jean-Sébastien , Zernecke Alma , Saliba Antoine-Emmanuel , Cochain Clément 
Published: August 2020

Rationale:
After myocardial infarction, neutrophils rapidly and massively infiltrate the heart, where they promote both tissue healing and damage.

Objective:
To characterize the dynamics of circulating and cardiac neutrophil diversity after infarction.

Methods and results:

We employed single-cell transcriptomics combined with cell surface epitope detection by sequencing to investigate temporal neutrophil diversity in the blood and heart after murine myocardial infarction. At day 1, 3, and 5 after infarction, cardiac Ly6G
+
(lymphocyte antigen 6G) neutrophils could be delineated into 6 distinct clusters with specific time-dependent patterning and proportions. At day 1, neutrophils were characterized by a gene expression profile proximal to bone marrow neutrophils (
Cd177
,
Lcn2
,
Fpr1
), and putative activity of transcriptional regulators involved in hypoxic response (
Hif1a
) and emergency granulopoiesis (
Cebpb
). At 3 and 5 days, 2 major subsets of
Siglecf

hi

(enriched for eg,
Icam1
and
Tnf
) and
Siglecf

low

(
Slpi, Ifitm1
) neutrophils were found. Cellular indexing of transcriptomes and epitopes by sequencing (CITE-seq) analysis in blood and heart revealed that while circulating neutrophils undergo a process of aging characterized by loss of surface CD62L and upregulation of
Cxcr4
, heart infiltrating neutrophils acquired a unique SiglecF
hi
signature. SiglecF
hi
neutrophils were absent from the bone marrow and spleen, indicating local acquisition of the SiglecF
hi
signature. Reducing the influx of blood neutrophils by anti-Ly6G treatment increased proportions of cardiac SiglecF
hi
neutrophils, suggesting accumulation of locally aged neutrophils. Computational analysis of ligand/receptor interactions revealed putative pathways mediating neutrophil to macrophage communication in the myocardium. Finally, SiglecF
hi
neutrophils were also found in atherosclerotic vessels, revealing that they arise across distinct contexts of cardiovascular inflammation.

Conclusions:

Altogether, our data provide a time-resolved census of neutrophil diversity and gene expression dynamics in the mouse blood and ischemic heart at the single-cell level, and reveal a process of local tissue specification of neutrophils in the ischemic heart characterized by the acquisition of a SiglecF
hi
signature.
Reasoning about Durations
Authors: Kelly Laura Jane , Khemlani Sangeet , Johnson-Laird P. N. 
Published: August 2020

Abstract
A set of assertions is consistent provided they can all be true at the same time. Naive individuals could prove consistency using the formal rules of a logical calculus, but it calls for them to fail to prove the negation of one assertion from the remainder in the set. An alternative procedure is for them to use an intuitive system (System 1) to construct a mental model of all the assertions. The task should be easy in this case. However, some sets of consistent assertions have no intuitive models and call for a deliberative system (System 2) to construct an alternative model. Formal rules and mental models therefore make different predictions. We report three experiments that tested their respective merits. The participants assessed the consistency of temporal descriptions based on statements using “during” and “before.” They were more accurate for consistent problems with intuitive models than for those that called for deliberative models. There was no robust difference in accuracy between consistent and inconsistent problems. The results therefore corroborated the model theory.
Causes of Interdecadal Increase in the Intraseasonal Rainfall Variability over Southern China around the Early 1990s
Authors: Cheng Yifeng , Wang Lu , Li Tim 
Published: August 2020

AbstractThe southern China (SC) summer rainfall exhibits prominent intraseasonal variability, which exhibits a significant increase in the early 1990s with the turning point at 1993. The SC intraseasonal rainfall events could be divided into three categories according to different propagations, including the southward-propagating (SP) events, the northwestward-propagating (NWP) events, and the northward-propagating (NP) events. This study explores the causes of the observed interdecadal increase in the intraseasonal rainfall variability over SC by comparing the SC intraseasonal rainfall events of each category between the former decadal period (P1) and the later decadal period (P2). The result indicates that such interdecadal change is due to the more frequent NP events coming from the South China Sea (SCS). Based on the moisture and vorticity budget analysis, it is revealed that the summer mean southerly wind in the middle to lower troposphere is the dominant factor of the northward propagation over the SCS, as it could induce positive meridional moisture and vorticity advection anomalies ahead of the convection. A marked interdecadal enhancement of the summer mean southerly wind over the SCS is the cause of more frequent occurrence of NP events over SC, as it provides more favorable conditions for the northward propagation. The change of the atmospheric instability over the SCS where the NP convection perturbation originates was also investigated, but no significant change was found.
A Critical Evaluation of the Adequacy of the Gamma Model for Representing Raindrop Size Distributions
Authors: Gatidis Christos , Schleiss Marc , Unal Christine , Russchenberg Herman 
Published: August 2020

AbstractThe adequacy of the gamma model to describe the variability of raindrop size distributions (DSD) is studied using observations from an optical disdrometer. Model adequacy is checked using a combination of Kolmogorov–Smirnov goodness-of-fit test and Kullback–Leibler divergence and the sensitivity of the results to the sampling resolution is investigated. A new adaptive DSD sampling technique capable of determining the highest possible temporal sampling resolution at which the gamma model provides an adequate representation of sampled DSDs is proposed. The results show that most DSDs at 30 s are not strictly distributed according to a gamma model, while at the same time they are not far away from it either. According to the adaptive DSD sampling algorithm, the gamma model proves to be an adequate choice for the majority (85.81%) of the DSD spectra at resolutions up to 300 s. At the same time, it also reveals a considerable number of DSD spectra (5.55%) that do not follow a gamma distribution at any resolution (up to 1800 s). These are attributed to transitional periods during which the DSD is not stationary and exhibits a bimodal shape that cannot be modeled by a gamma distribution. The proposed resampling procedure is capable of automatically identifying and flagging these periods, providing new valuable quality control mechanisms for DSD retrievals in disdrometers and weather radars.
HCC Liver Transplantation Wait List Dropout Rates Before and After the Mandated 6-Month Wait Time
Authors: Sokolich Julio , Buggs Jacentha , LaVere Michael , Robichaux Kobe , Rogers Ebonie , Nyce Samantha , Kumar Ambuj , Bowers Victor 
Published: August 2020

Background Studies have shown significant improvement in hepatocellular carcinoma (HCC) recurrence rates after liver transplantation since the united network of organ sharing (UNOS) implementation of a 6-month wait period prior to accrued exception model for end-stage liver disease (MELD) points enacted on October 8, 2015. However, few have examined the impact on HCC dropout rates for patients awaiting liver transplant. Our objective is to evaluate the outcomes of HCC dropout rates before and after the mandatory 6-month wait policy enacted. Methods We conducted a retrospective cohort study on adult patients added to the liver transplant wait list between January 1, 2012, and March 8, 2019 (n = 767). Information was obtained through electronic medical records and organ procurement and transplant network (OPTN) publicly available national data reports. Results In response to the 2015 UNOS-mandated 6-month wait time, dropout rates in the HCC patient population at our center increased from 12% pre-mandate to 20.8% post-mandate This increase was similarly reflected in the national dropout rate, which also increased from 26.3% pre-mandate to 29.0% post-mandate. Discussion From these changes, it is evident that the UNOS mandate achieved its goal of increasing equity of liver organ allocation, but HCC patients are nonetheless dropping off of the wait list at an increased rate and are therefore disadvantaged.
Dysphagia and Dysphonia, a Pairing of Symptoms Caused by an Unusual Pair of Diseases: Castleman’s Disease and Myasthenia Gravis
Authors: Abdelmeguid Ahmed , Rojansky Rebecca , Berry Gerald J. , Dewan Karuna 
Published: August 2020

Objectives: To describe a case of coincident Castleman’s disease and myasthenia gravis that initially presented as rapidly progressive dysphagia and dysphonia and to review the unique pathophysiology of these two uncommon diagnoses. Methods: Case report and literature review. Results: Castleman’s disease, angiofollicular or giant lymph node hyperplasia, is a rare benign lymphoid proliferation. Traditionally, the disease is classified based on histologic and clinical characteristics. Fewer than 10 cases with concurrent myasthenia gravis have been reported. Myasthenia gravis and thymic epithelial tumors are both associated with acetylcholine receptor antibody. While patients with isolated Castleman’s disease are usually asymptomatic, those who have concurrent myasthenia gravis and undergo surgical treatment are at increased risk of postoperative myasthenic crisis. Both pre- and postoperative plasmapheresis are suggested to improve muscle strength and prevent severe postoperative complications. Conclusions: In the setting of multiple cranial neuropathies including velopalatal insufficiency and bilateral ptosis it is important to consider myasthenia gravis. Castleman’s disease occurs rarely in conjunction with myasthenia gravis but may increase the risk of myasthenic crisis.
Tobacco prevention education for middle school and high school educators
Authors: Baer Caroline M , Khoussine Michaela A , Dobbs Page D 
Published: August 2020

Objectives: In an attempt to reduce tobacco use among adolescents in the USA, some health educators have created school-based tobacco education programmes. However, none currently exist for middle and high school educators. The current study sought to determine if middle and high school educators’ knowledge about combustible cigarettes and electronic cigarettes (e-cigarettes) improved following an educational session about youth tobacco use. Design: Pre-/post-assessment was employed to measure change in knowledge. Setting: Educators ( N = 41) from a rural, southern middle and high school were invited to take part in the study during an educational workshop held during an in-service training event. Method: In addition to seven knowledge questions, participants also provided demographic information and details of current and previous use of cigarettes and e-cigarettes. Chi-square analyses examined changes in responses to individual items, and total pre-/post-knowledge score change was compared using a dependent t-test. Independent t-tests examined differences between knowledge change and participants’ personal and behavioural characteristics. Results: Participants’ overall knowledge improved from pre- to the post-test ( p < .05); however, there was no relationship between pre-/post-test responses for individual items. In addition, there were no differences between participants’ knowledge change when compared by age, sex, grades taught and past use of cigarettes or e-cigarettes. Discussion: Increasing middle school and high school educators’ knowledge about tobacco products, particularly novel devices, is important due to the increasing rates of use among youth. More research is needed to determine how educators’ knowledge can help decrease tobacco initiation and use among school-aged children.
Association between body mass index and clinical characteristics, as well as with management, in Chinese patients with breast cancer
Authors: Wang Bin , Zhu Lizhe , Jiang Siyuan , Zhao Lizhi , Zhou Yuhui , Niu Ligang , Yan Yu , Wang Ke 
Published: August 2020

Objective Body mass index (BMI) is a risk factor associated with breast cancer in postmenopausal women. This study aimed to identify the associations of BMI with clinical characteristics and management of breast cancer in female Chinese patients. Methods Clinicopathological information on 1296 women who were diagnosed with breast cancer was collected at our hospital. We recorded the clinicopathological characteristics, molecular phenotypes, manner of diagnosis, implementation rate of preoperative examinations, and surgical method used. Results Significant differences were found in the tumor size, disease stage, manner of diagnosis, implementation rate of preoperative examinations, and the surgical method among different BMI groups. In premenopausal patients, significant differences were found in the distribution of molecular phenotypes and surgical approach among different BMI groups. In postmenopausal patients, different BMI groups showed significant differences in the tumor size, disease stage, distribution of molecular phenotypes, manner of diagnosis, rate of implementation of preoperative mammography, and surgical method. Conclusion Higher BMI is associated with a larger tumor size, more advanced disease stage, diagnosis by physical examination, higher implementation rate of preoperative examinations, and lower radical surgery rate in Chinese women with breast cancer. However, the relationship between BMI and molecular phenotypes differs between pre- and postmenopausal women.
Medial Elbow Instability Resulting From Partial Tears of the Ulnar Collateral Ligament: Stress Ultrasound in a Cadaveric Model
Authors: Ciccotti Michael C. , Hammoud Sommer , Dodson Christopher C. , Cohen Steven B. , Nazarian Levon N. , Ciccotti Michael G. 
Published: August 2020

Background: There is consensus that most complete ulnar collateral ligament (UCL) injuries in throwers would benefit from surgical intervention. Optimal treatment for partial UCL tears remains controversial. Stress ultrasonography has become a well-accepted diagnostic modality for assessing UCL injury. Hypothesis: Partial UCL tears will result in an intermediate increase in ulnohumeral joint space gapping as compared with that of an intact UCL and a complete UCL tear, but the degree of joint space gapping will vary by anatomic location of the partial tear. Study Design: Controlled laboratory study. Methods: Twenty-one cadaveric elbows were divided into 7 groups representing different anatomic locations of UCL partial tears. Partial tears were simulated by cutting 50% of the measured width of the UCL at 6 locations: distal anterior/posterior, midsubstance anterior/posterior, and proximal anterior/posterior. A seventh partial tear was created by partially elevating the undersurface of the distal UCL to simulate the radiographic “T-sign.” Valgus stress (15 daN) was applied to each cadaveric elbow at 30° of flexion using a standardized device. Each specimen was tested intact, partially torn, and completely torn. At each state, joint space was measured using stress ultrasonography, and the difference in joint space from unstressed to stressed (delta) was recorded. Results: There were 10 right and 11 left upper extremity specimens. The mean delta was 0.58 mm for the intact state and <0.75 mm in all groups. Both distal partial tear groups had mean deltas <0.75 mm, similar to intact elbows. Proximal tears and the T-sign demonstrated intermediate deltas (0.99-1.23 mm). Midsubstance partial tears demonstrated the largest deltas (1.57-2.03 mm), similar to those of the complete tears. All complete tear groups had a mean delta >1.5 mm (1.54-3.03 mm). Conclusion: These findings suggest that partial tears introduce a spectrum of instability from functionally intact to completely torn. As a result, some may be biomechanically amenable to nonoperative treatment, while others would be indicated for early reconstruction. Further research into the biological and biomechanical determinants of nonoperative treatment failure will assist with more precise treatment recommendations. Clinical Relevance: Describing the biomechanical consequences of different, clinically significant partial UCL tears potentially allows more precise recommendations for operative and nonoperative treatment.
Use and reporting of risk of bias tools in 825 systematic reviews of acupuncture: a cross-sectional study
Authors: Long Youlin , Wang Xin , Xiao Wenzhe , Chen Rui , Guo Qiong , Liu Jia , Shao Ruochen , Huang Jin , Du Liang 
Published: August 2020

Objective: To assess the use and reporting of risk of bias (RoB) tools in systematic reviews (SRs) of acupuncture. Study design and setting: We extracted and analyzed information relating to RoB in acupuncture SRs via Medline, Embase and the Chinese CNKI (Chinese National Knowledge Infrastructure), WanFang and VIP databases from their inception to 24 November 2017. Three subgroup analyses were used to check the influence of language, journal type and impact factor, following which we used descriptive analysis. Results: We included 825 acupuncture SRs, of which 48% used the Cochrane RoB tool. Only 36% used the latest version of the Cochrane Handbook (version 5.1.0 at time of writing) with higher proportions among Cochrane SRs (65%) versus non-Cochrane SRs (34%), and high impact factor journals (58%) versus low or no impact factor journals (28% and 38%, respectively). In the last decade, there were notable increases in the use of the Cochrane RoB tool and Cochrane Handbook version 5.1.0, of 43% and 19%, respectively. Chinese-language SRs demonstrated proportionally higher tendencies to report an incorrect Cochrane Handbook version, increasing by 14% in the last 5 years. Additionally, 7% SRs did not report any results, and only 10% reported relatively complete and adequate RoB assessment. Cochrane SRs reported more complete assessments than Chinese-language or non-Cochrane English-language SRs. Conclusion: Use and reporting of RoB tools were suboptimal. Proportionally, use of the Cochrane RoB tool and Cochrane Handbook version 5.1.0 was low but rising. Our results highlight the prevalence and concerns of using unsuitable tools and the issue of incomplete RoB reporting. RoB tool application requires further improvement.
Comparison of electroacupuncture and manual acupuncture for patients with plantar heel pain syndrome: a randomized controlled trial
Authors: Wang Weiming , Liu Yan , Jiao Ruimin , Liu Sixing , Zhao Jie , Liu Zhishun 
Published: August 2020

Background: Plantar heel pain syndrome (PHPS), also known as plantar fasciitis, affects millions of people worldwide. Electroacupuncture (EA) and manual acupuncture (MA) are the two acupuncture modalities frequently used for PHPS in the clinical setting. However, which modality is more effective has yet to be determined. Objective: To examine whether EA is more effective than MA with regards to pain relief for patients with PHPS. Methods: Participants were randomly assigned (1:1) to receive 12 treatment sessions of EA or MA over 4 weeks with 24 weeks of follow-up. The primary outcome was the proportion of treatment responders, defined as patients with at least a 50% reduction from baseline in the worst pain intensity experienced during the first steps in the morning after a 4-week treatment, measured using a visual analogue scale (VAS, 0–100; higher scores signify worse pain). Analysis was by intention-to-treat. Results: Ninety-two patients with a clinical diagnosis of PHPS were enrolled from 29 July 2018 through 28 June 2019. Of the patients, 78 (85%) completed the treatment and follow-up. The primary outcome occurred in 54.8% (23/42) of the EA group compared to 50.0% (21/42) of the MA group after the 4-week treatment (difference –4.76, 95% confidence interval, –26.10 to 16.57, P = 0.662). There were no significant between-group differences for any secondary outcomes after 4 weeks of treatment and at 16 weeks and 28 weeks of follow-up. There were no serious treatment-related adverse events in either group. Conclusion: Among patients with PHPS, EA did not have a better effect with respect to relieving pain intensity than MA at week 4, although both EA and MA appeared to have positive temporal effects, with decreased heel pain and improved plantar function. Trial registration number: ChiCTR1800016531 (Chinese Clinical Trial Registry).