bluuebunny/crossref_metadata_2025_split huggingface dataset:
Management of unexplained: poly-consultation and hyperfrequency on Primary Health Chilean Attention. A qualitative and exploratory study
Authors: Sánchez Nicolás Fuster , López Diego Rivera , Retamales Hugo Sir , Pérez Constanza Gómez , Torres Magdalena Delgado 
Published: July 2020

Abstract
Background: In Europe, Latin-America, and Asia, poly-consultation has become a complex problem for the management of different healthcare systems. However, in the current literature, little attention has been paid to exploring perspectives of territorial and critical analysis to manage unexplained symptoms.The purpose of this study is to analyze the socio-structural elements that underlie the users’ phenomenon of poly-consultation or hyperfrequency in the Chilean primary healthcare system (PHCS). Methods: This paper represents qualitative data collected as part of an exploratory study that used mixed methods across three metropolitan areas of Santiago, Valparaíso, and Concepción, Chile. The study involved a sample of 24 subjects from administrative and management positions in PHC who were recruited from Family Health Care Centers, considering urban municipalities from the low, medium, and high stratum.The study collected data using one set of semi-standardized interviews during a year. Data analysis used an qualitative content analysis.Results: This article shows that poly-consultant patients provide a critical clinic category to management and mental health model (foreshadowing a social topology base that promotes a determinate unspecific and diffuse experience on users) that cannot be cover by current biomedical models. Data showed the strain of a somatoform clinic category, especially in the clinic and epistemological exercise, and, also, the relevance of particularities of Chile, a country with a mixed health system characteristics and their effects: the naturalization of collective problems managed as individual problems. Conclusions: Results from the study have the potential to inform healthcare professionals and managers of strategies for developing effective and territorially based. We conclude that hyperfrequency and poly-consultation in Chile reveal relevant stratification in the territory who has particularities than could be studied from a quantitative perspective.
Live Birth Rate and Neonatal Outcomes of Different Quantities and Qualities of Frozen Transferred Blastocyst in Patients Requiring Whole Embryo Freezing Stratified by Age
Authors: Chen Shiping , Du Hongzi , Liu Jianqiao , Liu Haiying , Li Lei , He Yuxia 
Published: July 2020

Abstract
Background: Multiple pregnancies are associated with significant complications and health risks for both the mothers and infants. Single blastocyst transfer (SBT) is a logical and effective measure to reduce the incidence of multiple pregnancy with assisted reproductive technology (ART). Whether it is suitable for everyone undergoing SBT was inconclusive, in view of the consideration of embryo quality and patients’ age.Objective: To explored live birth rate (LBR) and neonatal outcomes of different quantities and qualities of blastocysts in patients stratified by age, using a cutoff of 35 years, who required whole embryo freezing and underwent a subsequent frozen-thawed transfer (FET) cycle.Methods: A total of 3362 patients were divided into five groups: group A (n=1569) received a single good-quality blastocyst; group B (n=1113) received two good-quality blastocysts; group C (n=313) received one good- and one average-quality blastocyst; group D (n=222) received two average-quality blastocysts; and group E (n=145) received one average-quality blastocyst. Results: For patients have good-quality blastocysts, irrespective of age, the LBR of double blastocyst transfer (DBT) were about 50-65% and multiple pregnancy rate (MPR) were 40-60%, however, the LBR of single blastocyst transfer (SBT) were 40-55% and MPR were 3.5-6.3%. For patients who only had average-quality blastocysts, the MPR of double average-quality blastocysts transfer was as high as 30-50%. Moreover, about 70-90% of preterm births resulted from multiple pregnancies, and about 85-95% of low birth weight babies come from multiple pregnancies. The neonatal outcomes (gestational age, birth weight and birth height ) of SBT were significantly lower than those of DBT regardless of age, and this statistical difference disappeared if the patients were sub-grouped by singleton or twin. There is no significant difference in neonatal outcomes between single good-quality blastocyst and single average-quality blastocyst transfer. Conclusions: SBT is a preferable option for patients regardless of age when good-quality blastocysts are available. For patients who only had average-quality blastocysts, patients should be informed that DBT was associated with higher multiple pregnancy and adverse neonatal outcomes when compared with SBT regardless of age, suggesting that the practice of SBT is also feasible for these patients.
Effect of miR-181b on the biological characteristics and clinical drug resistance of small cell lung cancer by targeting gene ACE2
Authors: Liu Huining , Su Jianling , Han Shaohui , Yin Qifan , Wang Yuexin 
Published: July 2020

Abstract
Objective To clarify the effect of miR-181b on the biological function of SCLC, to detect and verify its downstream target gene ACE2, and to explore the effect of clinical resistance on SCLC in order to find new specific diagnostic markers and therapeutic targets.Methods 1. Collect blood samples from 30 SCLC patients and 30 normal persons in our department from 2017 to 2019 to detect the expression level of miR-181b; 2. Detect the expression level of miR-181b in SCLC cells by RT-PCR, and Screening of downstream target genes used by gene chip, verification with luciferase and Western Blot; 3. Collect the general data of 30 SCLC patients and 30 healthy people (control group)，the patients were diagnosed by pathology and undergoing EC protocol in the Department of Thoracic Surgery and Oncology of our hospital to detect the expression level of mir-181b in different periods; 4. In the SCLC cell line and the SCLC mouse model constructed, different concentrations of EC chemotherapy and ACEI drugs were administered to detect the sensitivity of drug resistance and non-drug resistance.Results 1. The expression level of miR-181b in SCLC patients was lower than normal people; 2. The expression level of miR-181b in SCLC cell lines was lower than normal cells; ACE2 was verified as a downstream target of miR-181b by gene chip screening; 3. miR-181b is low-expressed in SCLC patients, first-line chemotherapy can promote its recovery, but cannot repair to normal levels, and miR-181b has a certain effect on SCLC chemotherapy sensitivity; 4. miR-181b can enhance the drug sensitivity of SCLC drug-resistant cells, the application of ACEI should increase the risk of SCLC drug-resistant.Conclusion 1. miR-181b has a low expression level in SCLC, and it can directly target the gene ACE2 to affect the biological characteristics of SCLC; 2. miR-181b is lowly expressed in SCLC patients, and first-line chemotherapy can promote it recovery, but cannot repair to normal levels, and ACEI drugs can increase the risk of SCLC drug-resistant.
Signal Reconstruction with Semidefinite Relaxation Optimization in Uplink Massive MIMO Systems
Authors: Li Guo 
Published: July 2020

Abstract
In this paper, we consider a single group massive MIMO system, which consists of one receiver (base station) deployed with a large number of antennas and multiple users with each having single antenna. In the uplink transmissions from users to base station, we focus on an efficient signal reconstruction of all the users' transmitted symbols. We build a statistic decision matrix based on the received signals, whose eigenvectors and eigenvalues constitute the key components of our signal reconstruction algorithm. Then, an optimization problem is formulated and we convert this problem to two sub-problems: obtaining the optimal weights by solving Semidefinite Relaxation (SDR) optimization and obtaining the optimal rotation phases. Finally, an iterative algorithm framework is proposed for multi-user signal reconstruction. Numerical results are also carried out to verify the bit-error-ratio (BER) performance compared with the several coherent detection schemes and the noncoherent transmission schemes.
Value of endobronchial cryotherapy in the management of malignant endobronchial obstruction in patients with inoperable NSCLC: A prospective analysis of clinical and survival outcomes
Authors: Rashad Alaa , Badawy Mohamed Shahat , Ali Mohammed Mustafa , Mansour Haggagy , Bary Mohamed Abdel 
Published: July 2020

Abstract
Background: Malignant endobronchial obstruction (MEBO) is the most debilitating complication in non-small cell lung cancer (NSCLC). The therapeutic role of cryotherapy and its impact on survival has not been well addressed. This is to clarify whether the combination of endobronchial cryotherapy (EBCT) and chemoradiotherapy (CRT) improved the outcomes in inoperable NSCLC patients with symptomatic MEBO compared to that obtained by CRT alone.Methods: A prospective ranomized cohort study included 60 cases presented to Qena University Hospital, Egypt, between December 2016 and May 2019. Group A included 30 patients were managed with EBCT plus CRT. Group B included 30 patients were managed with CRT alone. The outcomes assessed were symptoms relief, respiratory function tests (RFT), performance status and survival outcomes at baseline and 4 weeks of follow-up.Results: Group A patients showed a highly significant improvement in symptoms, RFT, 6MWD test and arterial blood gases, compared to group B. The mean Karnofsky score increased from 57.33±5.67 % at baseline to 60.67±6.39 % post-EBCT (P=0.036); group A was significantly improved compared to group B (P=0.04). The Kaplan-Meier median survival for all patients was 9.7±0.4 months (95% CI= 8.86-10.54), and group A cases (10.77±0.44 months, 95% CI= 9.9 -11.6) was significantly longer than that of group B cases (8.6±0.68 months, 95% CI= 7.3-9.97; T-test = 2.631, P= 0.011). Conclusion: The use of EBCT with CRT for the management of MEBO in symptomatic patients with inoperable NSCLC is an efficient and safe procedure. EBCT improves the clinical outcomes, RFT, performance status and median survival.
Serum IGFBP-2 in Systemic Sclerosis as a Protective Factor of Lung Dysfunction
Authors: Guiot Julien , Njock Makon-Sébastien , André Béatrice , Gester Fanny , Henket Monique , Seny Dominique de , Moermans Catherine , Malaise Michel G. , Louis Renaud 
Published: July 2020

Abstract
Background Systemic sclerosis (SSc) is a rare connective tissue disease associated with rapid evolving interstitial lung disease (SSc-ILD), driving its mortality. Specific biomarkers associated with the progression of this lung disease are highly needed. We aimed to identify specific biomarkers of SSc-ILD to predict the evolution of the disease.Methods We compared prospectively serum levels of several biomarkers associated with lung fibrosis in SSc patients (n = 102) compared to healthy subjects (HS) (n = 39). We also performed a longitudinal study in a subgroup of 28 patients analyzing biomarkers variations and pulmonary function tests over a period of 2 years. Furthermore, we performed in vitro analysis to study the impact of Insulin like Growth Factor Binding Protein (IGFBP)-2 on fibrotic activity of human lung fibroblasts.Results Serum levels of IGFBP-1, IGFBP-2, interleukin-8 and matrix metallopeptidase-9 were significantly increased in SSc patients compared to HS while IGF-1 and IGFBP-3 were reduced. The variation of IGFBP-2 between baseline and 2-year follow-up was positively correlated with pulmonary function (assessed by carbon monoxide transfer coefficient (KCO)) at 2-year follow-up (r = 0.6, p < 0.001). Receiver operating characteristic curve analysis enabled us to identify that baseline IGFBP-2 < 105 ng/ml was associated with a better outcome (low risk to display KCO < 70% predicted) at 2-year follow-up (area under the curve = 0.75 at 75% sensibility and 68% specificity, p < 0.05). In vitro functional study showed that IGFBP-2 significantly reduced fibroblast proliferation and pro-fibrotic activity.Conclusions We showed for the first time that serum levels of IGFBP-2 might predict the evolution of SSc-ILD. Baseline IGFBP-2 above 105 ng/ml might be a prognostic factor of alveolo-capillary dysfunction.
Research on Active Compounds of Huanglian Jiedu Decoction for the Treatment of Corona Virus Disease 2019 Based on Network Pharmacology and Molecular Docking
Authors: Liu Leping , Xu Xinyi , Cao Xueshuai , Long Xi , Luo Yanwei , Gui Rong 
Published: July 2020

Abstract
Background Huanglian Jiedu Decoction (HLJDD) is a traditional Chinese prescription for the treatment of influenza, inflammation and other ailments related to heat-syndrome, a typical pathological symptom in Traditional Chinese Medicine. It was recommended as one of the basic prescriptions among the Proposed Diagnoses and Treatment issued by China’s National Health Commission. In this work we investigated the molecular mechanism of action of Huanglian Jiedu Decoction in the treatment of Corona Virus Disease 2019 (COVID-19) through network pharmacology and molecular docking approaches. Methods The chemical constituents and action targets of Coptis chinensis, Scutellaria baicalensis, Phellodendron amurense, Gardenia jasminoides in HLJDD were retrieved on Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP). The database of UniProt and GeneCards were used to query the target genes that corresponding to the active compounds, and then a compound-target network was constructed using Cytoscape 3.7.2. GO database was used to annotate GO functions. Reactome was used to analyze KEGG enrichment pathway, predicting the possible mechanisms of active compounds. DAVID database was used to analysis the tissue enrichment. The main active ingredient is molecularly docked with the SARS-CoV-2, ACE2 and TMPRSS2. Results We screened 84 compounds and obtained 341 corresponding target genes in the network. Gene annotation showed that the targets were involved mainly in 457 biological functions. 306 signaling pathways was enriched, involving chemokine and cytokine signaling pathways that mediate inflammation, interferon-γ signaling pathway, p53 pathway. And the targets mainly distributed in the lung liver and placenta, involving a variety of immune cells, such as T cells, B cells. The molecular docking results showed that core compounds such as beta-sitosterol, stigmasterol and quercetin had high affinity with SARS-CoV-2, ACE2 and TMPRSS2, which was comparable with drugs like abidol used to COVID-19 treatment by. Conclusions The active compounds in HLJDD may have a therapeutic effect on COVID-19 through regulating multiple signal pathways by targeting genes such as VEGF, NOS2, IL6, MMP9, IL10, and TGFB1.
Dietary Intake and Hypertension Risk: A Cross-Sectional Survey Using Data from the China hypertension survey, 2012-2015
Authors: Wu Xingwei , Wu Yu , Yong Zhengping , Hou Zhengyao , Zhang Yueqi , Cai Li , Liu Zhou , Tang Yijia , Tong Rongsheng , Wan Shaoping 
Published: July 2020

Abstract
Background: Available data about the effects of dietary intake on hypertension remains limited and conflicting. The purpose of this study was to assess the correlation between dietary intake and the incidence of hypertension. Methods: The China Hypertension Survey was conducted nationwide, and the data of 19,277 residents from Sichuan was organized, and finally 2,010 samples were selected into this study. Standard food frequency questionnaires were used to collect dietary intake data and physical examinations including blood pressure were performed on site. The subjects were classified into hypertensive group (n=356) and non-hypertensive group (n=1,654). The odds ratio (OR) for the occurrence of hypertension according to the quartiles of the dietary intake was evaluated by multivariable logistic regression. Results: After adjustment for all potential confounding variables, more than 50g of daily egg intake was a risk factor for hypertension prevalence (OR=2.11; 95% CI: 1.34-3.32), and 1.94-3.33g of animal oil intake per day was a protective factor (OR=0.57; 95% CI: 0.37-0.88). Conclusions: Our findings demonstrate a significant positive correlation between eggs above 50g and the risk of hypertension, while 1.94-3.33g of animal oil is negatively correlated with the risk of hypertension, indicating that adherence to a small amount of eggs and an appropriate amount of animal oil intake have beneficial effects on the prevention of hypertension.
Fructooligosaccharides improves growth performance and intestinal epithelium function in weaned pigs exposure to Enterotoxigenic Escherichia coli
Authors: Liu Lei , Chen daiwen , Yu Bing , Heng Yin , Huang Zhiqing , Luo Yuheng , Zheng Ping , Mao Xiangbing , Yu Jie , Luo Junqiu , Yan Hui , He Jun 
Published: July 2020

Abstract
Background

This study was conducted to explore the protective potential of Fructooligosaccharides (FOS) against Enterotoxigenic Escherichia coli (ETEC)-induced inflammation and intestinal injury in weaned pigs.
Methods

Twenty-four weaned pigs were randomly assigned into three groups: (1) non-challenge (CON), (2) ETEC-challenge (ECON), and (3) ETEC challenge + 250 mg/kg FOS (EFOS). On day 19, non-challenged pigs were orally infused with sterilized culture while pigs in other groups were orally infused with ETEC (2.5 × 1010 colony-forming units). After 3 days, pigs were slaughtered for sample collection.
Results

We show that ETEC-challenged significantly reduced average daily gain (ADG); however, FOS improved the ADG (P < 0.05) and apparent digestibility of crude protein (CP), gross energy (GE), and ash in the ETEC-challenged pigs (P < 0.05). FOS reduced plasma concentrations of IL-1β and TNF-α, and elevated the concentrations of immunoglobulin A (IgA) and immunoglobulin M (IgM) (P < 0.05). Interestingly, FOS elevated villus height in duodenum, and elevated the ratio of villus height to crypt depth in the duodenum and ileum in the ETEC-challenged pigs (P < 0.05). Moreover, FOS increased lactase activity in the duodenum and ileum (P < 0.05). The activities of sucrase and alkaline phosphatase (AKP) were higher in EFOS group than in the ECON group (P < 0.05). Importantly, FOS up-regulated the expressions of critical genes in intestinal epithelium function such as zonula occludens-1 (ZO-1), L-type amino acid transporter-1 (LAT1), and cationic amino acid transporter-1 (CAT1) in the duodenum and the expressions of ZO-1 and glucose transporter-2 (GLUT2) in the jejunum (P < 0.05). FOS also up-regulated the expressions of occludin, fatty acid transporter-4 (FATP4), sodium glucose transport protein 1 (SGLT1), and GLUT2 in the ileum (P < 0.05). FOS significantly increased the concentrations of acetic acid, propionic acid and butyric acid in the cecal digesta. Additionally, FOS reduced the populations of Escherichia coli, but elevated the populations of Bacillus and Bifidobacterium in the caecal digesta (P < 0.05).
Conclusions

These results suggested that FOS can improve the growth performance and intestinal health in weaned pigs upon ETEC challenge, which was associated with suppressed inflammatory responses and improved intestinal epithelium functions and microbiota.
High order semi-implicit weighted compact nonlinear scheme for the all-Mach isentropic Euler system
Authors: Jiang Yanqun , Chen Xun , Zhang Xu , Xiong Tao , Zhou Shuguang 
Published: July 2020

Abstract
The computation of compressible flows at all Mach numbers is a very challenging problem. An efficient numerical method for solving this problem needs to have shock-capturing capability in the high Mach number regime, while it can deal with stiffness and accuracy in the low Mach number regime. This paper designs a high order semi-implicit weighted compact nonlinear scheme (WCNS) for the all-Mach isentropic Euler system of compressible gas dynamics. To avoid severe CFL restrictions for low Mach flows, the nonlinear fluxes in the Euler equations are split into stiff and non-stiff components. A third-order implicit-explicit (IMEX) method is used for the time discretization and a fifth-order WCNS is used for the spatial discretization. The designed semi-implicit WCNS is asymptotic preserving and asymptotically accurate in the zero Mach number limit. One- and two-dimensional numerical examples in both compressible and incompressible regimes are given to demonstrate the advantages of the designed method.
Dissecting Molecular Mechanisms Underlying H2O2-induced Apoptosis of Mouse Bone Marrow Mesenchymal Stem Cell: Role of Mst1 Inhibition.
Authors: Zhang Qian , Cheng Xianfeng , Zhang Haizhou , Zhang Tao , Wang Zhengjun , Zhang Wenlong , Yu Wancheng 
Published: July 2020

Abstract
Background
Bone marrow mesenchymal stem cell (BM-MSC) has been shown to treat pulmonary arterial hypertension (PAH). However, excessive reactive oxygen species (ROS) increases the apoptosis of BM-MSCs, leading to poor survival and engraft efficiency. Thus, improving the ability of BM-MSCs to scavenge ROS may considerably enhance the effectiveness of transplantation therapy. Mammalian Ste20-like kinase 1 (Mst1) is a pro-apoptotic molecule which increases ROS production. The aim of this study is to uncover whether Mst1 inhibition enhanced the tolerance of BM-MSCs under H2O2 condition and the underlying mechanisms.
Methods
Mst1 expression in BM-MSCs was inhibited via transfection with adenoviruses expressing a short hairpin (sh) RNA directed against Mst1 (Ad-sh-Mst1) and exposure to H2O2. Cell viability was detected by Cell counting Kit 8 (CCK‑8) assay, and cell apoptosis was analyzed by Annexin V-FITC/PI, Caspase 3 Activity Assay kits, and pro caspase 3 expression. ROS level was evaluated by the ROS probe DCFH-DA, mitochondrial membrane potential (ΔΨm) assay, SOD1/2, CAT, and GPx expression. Autophagy was assessed using transmission electron microscopy, stubRFP-sensGFP-LC3 lentivirus and autophagy-related protein expression. The autophagy/Keap1/Nrf2 signal in H2O2-treated BM-MSC/sh-Mst1 was also measured.
Results
Mst1 inhibition reduced ROS production, increased antioxidant enzyme SOD1/2, CAT, GPx expression, maintained ΔΨm, and alleviated cell apoptosis in H2O2-treated BM-MSCs. In addition, this phenomenon was closely correlated with the autophagy/Keap1/Nrf2 signal pathway. The autophagy inhibitor, the antioxidant pathway Keap1/Nrf2, was also blocked when autophagy was inhibited by 3-MA. However, Keap1 or Nrf2 knockout via siRNA had no effect on autophagy activation or suppression.
Conclusion
Mst1 inhibition mediates the cytoprotective benefit of mBM-MSCs against H2O2 oxidative stress injury. The underlying mechanisms involve autophagy activation and the Keap1/Nrf2 signal pathway.