bluuebunny/crossref_metadata_2025_split huggingface dataset:
Protocol for a Randomized Control Trial of the Caregiver Support Intervention with Syrian Refugees in Lebanon
Authors: Miller Kenneth E , Arnous Maguy , Tossyeh Fadila , Chen Alexandra , Bakolis Ioannis , Koppenol-Gonzalez Gabriela V. , Nahas Nayla , Jo Mark J.D. 
Published: November 2019

Abstract
Background Chronic stress has been shown to negatively impact parenting among refugees and other war-affected communities. Persistent parental stress and distress may lead to unresponsive, anxious, or overly harsh parenting and a corresponding increase in emotional and behaviour problems among children. Most parenting interventions emphasize the acquisition of knowledge and skills; however, this overlooks the deleterious effects of chronic stress on parenting. The Caregiver Support Intervention (CSI) aims to strengthen quality of parenting skills by lowering stress and improving psychosocial wellbeing among refugee parents of children aged 3-12, while also increasing knowledge and skills related to positive parenting. The CSI is a 9-session psychosocial group intervention delivered by non-specialist providers. It is intended for all adult primary caregivers of children in high-adversity communities, rather than specifically targeting caregivers already showing signs of elevated distress.Methods The primary objective of this study is to assess the effectiveness of the CSI through a parallel group randomized controlled study with Syrian refugee families in North Lebanon. Participants will be primary caregivers of children ages 3-12, with one index child per family. Families will be randomized to the CSI or a waitlist control condition. A total of 240 families (480 caregivers) will be recruited into the study. Randomization will be at the family level, and CSI groups will be held separately for women and men. The study will be implemented in two waves. Outcomes for both arms will be assessed at baseline, post-intervention, and at a three-month follow-up. The primary outcome is quality of parenting skills. Secondary outcomes include parental warmth and sensitivity, harsh parenting, parenting knowledge, and child psychosocial wellbeing. Putative mediators of the CSI on parenting are caregiver stress, distress, psychosocial wellbeing, and stress management.Discussion This trial may establish the Caregiver Support Intervention as an effective intervention for strengthening parenting in families living in settings of high adversity, particularly refugee communities.
Application of Multidisciplinary Intervention Model in Nursing of Older patients with Diabetes
Authors: Zhao Zhonghong , Wang Xiaoyun 
Published: November 2019

Abstract
Objective To evaluate the application of multidisciplinary treatment (MDT) model in nursing of elderly patients with diabetes.Methods A total of 166 elderly diabetic patients were retrospectively selected. They were randomly and equally divided into the MDT group and the control group. The patients in the control group were treated with the traditional nursing method. And the patients in the MDT group were treated with a multidisciplinary team. The hospitalization time, hospital costs, incidence of diabetes-related complications, and nursing satisfaction were analyzed and compared.Results The hospitalization time and hospitalization costs were significantly lower in the MDT group than those in the control group (P < 0.05). The satisfaction rate of MDT group (98.3%) was significantly higher compared with the control group (86.6%; P < 0.05). Besides, the incidence of complications was significantly lower in the MDT group (10.8%) when comparing to the control group (40.1%; P < 0.05).Conclusion The application of MDT model in elderly diabetic care was superior to traditional routine nursing model, shortening the hospital stay and hospitalization costs, decreasing the incidence of complications and improving the nursing satisfaction of patients and their families, thereby improving the long-term prognosis and life quality of elderly diabetic patients.
A cross-sectional study of the relationships between different components of sarcopenia and brachial ankle pulse wave velocity in community-dwelling elderly
Authors: Rong Yu-Dong , Bian Ai-Lin , Hu Hui-Ying , Ma Yue , Zhou Xin-Zi 
Published: November 2019

Abstract
Background Previous studies have just found skeletal muscle mass decline is associated with arterial stiffness, but it is unclear whether muscle strength and physical performance as important compositions of sarcopenia are associated with arterial stiffness.The aim of this study was to investigate the relationship between sarcopenia, the components of sarcopenia and arterial stiffness among elderly in the community. Methods This study enrolled 450 elderly people who received general medical examinations in Tianjin First Central Hospital. Each of the subjects was greater than 65 years old, including 266 male and 184 female subjects. Based on the diagnostic criteria for sarcopenia in older people developed by the Asian Working Group for Sarcopenia (AWGS), 89 subjects were separated into the sarcopenia group. The living habits, disease status, general status and laboratory examinations of all subjects were collected. The body composition (including appendicular skeletal muscle mass and visceral fat area (VFA) of each participant) was measured by bioimpedance analysis. HS, usual gait speed (GS), and brachial ankle pulse wave velocity (baPWV) were measured.Results Sarcopenia subjects had higher baPWV, nutrition risk and lower appendicular skeletal muscle index(ASMI), Handgrip strength(HS), GS, body mass index(BMI), triacylglycerol(TG), serum albumin(ALB) and creatinine(Cr) than did non-sarcopenia subjects; Sarcopenia subjects also had higher visceral fat area (VFA) than did non-sarcopenia subjects (p<0.05). ASMI and HS were negatively correlated with baPWV (t=-5.807, p=0.000 and t=-3.085, p=0.002), but the relationship between baPWV and GS was not statistically significant (t=-0.862, p=0.389) by multivariable linear regression. After adjusting for confounders, a multivariate logistic regression analysis revealed that incidence of sarcopenia was related with age, BMI, sports and baPWV in community dwelling elderly. Conclusions ASMI and HS were negatively correlated with baPWV in community dwelling elderly in China; and baPWV was a risk factor for incidence of sarcopenia.
Gut microbiome-derived lactate promotes to anxiety-like behaviors through GPR81 receptor-mediated lipid metabolism pathway
Authors: Shan Baixi , Song Yonggui , Zeng Sufen , Song Jiagui , Zeng Qiang , Liao Zhou , Wang Tingting , Huang Chao , Su Dan 
Published: November 2019

Abstract
Accumulating evidence suggests that chronic stress can perturb the composition of the gut microbiota and induce host anxiety-like behavior. In particular, microorganism-derived products that can directly or indirectly signal to the nervous system. This study sought to investigate whether high levels of Lactobacillus and lactic acid in the gut of rats under chronic unpredictable stress were the factors leading to anxiety. Results: Oral lactic acid rat and transplantation of fecal microbiota from anxious rats into antibiotic-treated rat caused behavioral abnormalities such as psychomotor hyperactivity, impaired learning and memory in the recipient animals. These rats also showed inhibition of the adenylate cyclase-protein kinase A pathway of lipolysis after activation of G protein-coupled receptor 81 (GPR81) by lactic acid in the liver, as well as increased TNF-α, compared with healthy controls. Furthermore, we show that sphingosine-1-phosphate receptor 2 (S1PR2) protein expression in hippocampus is reduced in chronic unpredictable stress compared to control subjects and its expression negatively correlates with symptom severity. Conclusions: our study suggests that the gut microbiome-derived lactate promotes to anxiety-like behaviors through GPR81 receptor-mediated lipid metabolism pathway.
Vaginal microbiota alterations in women with recurrent implantation failure and the associated metabolome
Authors: Fu Min , Zhang Xiaowei , Qian Weiping , Liang Yiheng , Lin Shouren , Fan Shangrong 
Published: November 2019

Abstract
In vitro fertilization-embryo transfer (IVF-ET) is now widely applied in treating infertility. As the number of IVF cycles continues to increase, recurrent implantation failure (RIF) has become a big challenge. The cause of RIF is very complex and remains largely unrevealed, especially for those without any pathological features. It has been proved that vaginal microbiota is associated with many female reproductive diseases, such as pregnancy-related diseases, sexually transmitted diseases, tubal factor infertility, and first trimester miscarriage after in vitro fertilization (IVF) and so on. Hence, vaginal microbiota and its metabolome may also relate to RIF. In this study, we characterized the vaginal microbiota and metabolome of patients with unexplained RIF, while patients who achieved clinical pregnancy in the first IVF cycle were used as controls. Results Based on 16S rDNA sequencing of the vaginal microbiota, the RIF group presented higher microbial α-diversity than the control group (0.80±0.50 vs 0.50±0.39, P-value=0.016) and harbored more non-Lactobacillus microorganisms, including 25 significantly increased genera of both aerobic and anaerobic bacteria. The metabolomic profile showed that the relative abundances of 37 metabolites among 2,507 metabolites were significantly different between the two groups. Among them, 2',3-cyclic UMP and phosphoinositide were the top two metabolites significantly upregulated in the RIF group, while glycerophospholipids and benzopyran were important metabolites that were significantly downregulated. Lysobisphosphatidic acid (LPA) and prostaglandin (PG) metabolized from glycerophospholipids are key factors affecting implantation and decidualization. Benzopyran, as a selective estrogen receptor modulator (SERM), may affect the outcome of pregnancy. All of the metabolome outcomes may result in or from the differential microbiota composition in the RIF patients. Conclusions In conclusion, significant differences were presented in the vaginal microbiota and metabolome between RIF patients and women who became pregnant in the first IVF cycle, which are related to embryo implantation. This study not only deeply investigates the relationship between RIF and the vaginal microbial community and its metabolites but also provides a profound understanding of the pathogenesis of RIF.
Fast, simple and highly specific molecular detection of Vibrio alginolyticus pathogenic strains using a visualized isothermal amplification method
Authors: Dong Yu , Zhao Panpan , Wu Huahua , Shen Xin , Shen Hui , Li Juan , Qiao Yi , Chen Qiong , Jia Weiwei , Zhang Kunxiao , Dong Jingquan , Gao Song 
Published: November 2019

Abstract
Background: Vibrio alginolyticus is an important pathogen that has to be closely monitored and controlled in the mariculture industry for its strong pathogenicity, quick onset after infection and high mortality rate to aquatic animals. Fast, simple and specific methods are demanded for its on-site detection to effectively control its outbreaks and prevent economic losses. Detection specificity towards the pathogenic strains has to be emphasized to facilitate pointed treatment and prevention. Polymerase chain reaction (PCR) based molecular approaches have been developed, but their application is limited due to the requirement for complicated thermal cycling machines and trained personnel. Results: A fast, simple and highly specific detection method for V. alginolyticus pathogenic strains was established based on the isothermal recombinase polymerase amplification (RPA) and lateral flow dipsticks (LFD). The method targeted a virulence gene toxR that was reported to have a good coverage for the V. alginolyticus pathogenic strains. To ensure the specificity, the primer-probe set of the RPA system was carefully designed to recognize regions in gene toxR that were diverged in different Vibrio species but conserved in V. alginolyticus pathogenic strains. The primer-probe set was determined after a systematic screening on amplification performance, primer-dimer formation and false positive signals.The RPA-LFD method was confirmed to have a high specificity to V. alginolyticus pathogenic strains without any cross reaction with other Vibrio species, and was able to detect as low as 1 colony forming unit per microliter of the bacterium without DNA extraction. The method finishes detection within 30 min under the temperature between 35oC and 45oC, and the visual signal on the dipstick was directly read with naked eye. In an application simulation, randomly infected shrimp homogenate samples were 100% accurately detected. Conclusions: ​The RPA-LFD method developed in this study is fast, simple, highly specific and independent of complicated equipment. It is well applicable to the on-site detection of V. alginolyticus pathogenic strains for the mariculture industry.
Identifying Phenogroups in Patients with Subclinical Diastolic Dysfunction Using Unsupervised Statistical Learning
Authors: Strande Jennifer , Kaptein Yvonne E. , Karagodin Ilya , Zuo Hongquan , Lu Yu , Zhang Jun , Kaptein John S. 
Published: November 2019

Abstract
Subclinical diastolic dysfunction is a precursor for developing heart failure with preserved ejection fraction (HFpEF); yet not all patients progress to HFpEF. Our objective was to evaluate clinical and echocardiographic variables to identify patients who develop HFpEF.Methods and Results Clinical, laboratory, and echocardiographic data were retrospectively collected for 81 patients without HF and 81 matched patients with HFpEF at the time of first documentation of subclinical diastolic dysfunction. Unsupervised clustering identified 3 subgroups which differed in gender composition, severity of cardiac hypertrophy and aortic stenosis, NT-proBNP, percentage of patients who progressed to HFpEF, and timing of disease progression from diastolic dysfunction to HFpEF to death. Clusters that had higher percentages of women had progressively milder cardiac hypertrophy, less severe aortic stenosis, lower NT-proBNP, were diagnosed at an older age with HFpEF, and survived to an older age. Independent predictors of HFpEF for the entire cohort included diabetes, chronic kidney disease, atrial fibrillation, and diuretic use, with additional predictive variables found for each cluster.Conclusions Cluster analysis can identify phenotypically distinct subgroups of patients with diastolic dysfunction. Clusters differ in HFpEF and mortality outcome. In addition, the variables that correlate with and predict HFpEF outcome differ among clusters.
Transcriptomic and proteomic analyses between small yellow follicles and the smallest hierarchical follicles reveal a role of VLDLR in chicken follicle selection
Authors: Chen Qiuyue , Wang Yiya , Liu Zemin , Guo Xiaoli , Sun Yi , Kang Li , Jiang Yunliang 
Published: November 2019

Abstract
Background Follicle selection in chicken refers to the process of selecting one from a group of small yellow follicles (SY, 6-8mm in diameter) to enter the 12-15 mm hierarchical follicles (usually F6 follicles), which is a an important process affecting laying performance in the poultry industry. Although transcripromic analysis on chicken ovarian follicles was reported, integrated analysis on chicken follicles around selection by using both transcripromic and proteomic approaches was still lacking. In this study, we compared the proteomes and transcriptomes of SY and F6 follicles of laying hens and found some genes involved in chicken follicle selection.Results Transcriptomic analysis revealed 855 differentially expressed genes (DEGs) between SY follicles and F6 follicles of laying hens, among which 202 were upregulated and 653 were downregulated. Proteomic analysis revealed 259 differentially expressed proteins (DEPs), including 175 upregulated and 84 downregulated proteins. Among the identified DEGs and DEPs, the expression changes of seven genes including VLDLR1,WIF1, NGFR, AMH, BMP15, GDF6 and MMP13 , and nine proteins including VLDLR, VTG1, VTG3, PSCA, APOB, APOV1, F10, ZP2 and ZP3L2 were validated. In addition VLDLR expression was significantly down-regulated in F6 follicles compared with SY follicles, was signifcantly higher in the GCs than in the TCs and was stimulated by FSH in GCs of both hierarchical and prehierarchical follicles.Conclusions By comparing the proteomes and transcriptomes of SY follicles and F6 of laying hens, we identified some differentially expressed proteins/genes that might play certain roles in chicken follicle selection. These data may contribute to identification of the functional genes and proteins involved in chicken follicular development and selection.
Expert Curation of the Human and Mouse Olfactory Receptor Gene Repertoires Identifies Conserved Coding Regions Split Across Two Exons.
Authors: Barnes If , Ibarra-Soria Ximena , Fitzgerald Stephen , Gonzalez Jose , Davidson Claire , Hardy Matthew , Manthravadi Deepa , Gerven Laura Van , Jorissen Mark , Zeng Zhen , Khan Mona , Mombaerts Peter , Harrow Jennifer , Logan Darren , Frankish Adam 
Published: November 2019

Abstract
Olfactory receptor (OR) genes are the largest multi-gene family in the mammalian genome, with over 850 in human and nearly 1500 genes in mouse. The expansion of the OR gene repertoire has occurred through numerous duplication events followed by diversification, resulting in a large number of highly similar paralogous genes. These characteristics have made the annotation of the complete OR gene repertoire a complex task. Most OR genes have been predicted in silico and are typically annotated as intronless coding sequences. Here we have developed an expert curation pipeline to analyse and annotate every OR gene in the human and mouse reference genomes. By combining evidence from structural features, evolutionary conservation and experimental data, we have unified the annotation of these gene families, and have systematically determined the protein-coding potential of each locus. We have defined the non-coding regions of many OR genes, enabling us to generate full-length transcript models. We found that 13 human and 41 mouse OR loci have coding sequences that are split across two exons. These split OR genes are conserved across mammals, and are expressed at the same level as protein-coding OR genes with an intronless coding region. Our findings challenge the long-standing and widespread notion that the coding region of a vertebrate OR gene is contained within a single exon.
Generalized Joint Hypermobility among School-going children in Majmaah region, Saudi Arabia
Authors: Sirajudeen Mohamed Sherif , Waly Mohamed , Alqahtani Mazen , Alzhrani Msaad , Muthusamy Hariraja , Unnikrishnan Radhakrishnan , Saibannavar Rashmi , Alrubaia Wafa , Vaiyapuri Anandh , Nambi Gopal S 
Published: November 2019

Abstract
Background Generalized Joint Hypermobility (GJH) is common among schoolchildren and usually benign. However, progressively may lead to joint pain and developmental delay. Identifying the GJH at the school-age would facilitate to monitor early changes and to plan for early rehabilitative intervention. Hence, we aimed to determine the prevalence, pattern, and factors associated with the GJH among school-going children in the Majmaah region, Saudi Arabia. Methods The school children of both genders aged 8 to 14 years from the Majmaah region of Saudi Arabia participated in this cross-sectional study. The Beighton’s score was used to assess the joint hypermobility. Personal characteristics like age, height, weight, Body mass index (BMI), and handedness was also collected. Descriptive statistics were produced for personal characteristics, the point prevalence of hypermobility, frequency of Beighton score distribution, and prevalence of GJH. The association between specific factors and the presence of GJH was analyzed using Chi-square tests and logistic regression measures. Results The occurrence of GJH as defined by Beighton cutoff score ≥ 4 in the 303 participated primary school children was 15.2%. The prevalence of GJH was high among females (16.8%) compared to males (13.4%), but this difference is not statistically significant. Younger male children (age 8 to 11 years) were 16.7 times more probable to develop hypermobility (adjusted OR 0.06 [95% CI 0.01 – 0.56]) compared to older male children (age 12 to 14 years). No associations were observed among female children. Conclusion The prevalence rate reported in this study among school-going children was in the lower range in comparison with the rates reported across the globe. Beighton score of ≥4 was adequate for use in identifying GJH among children in Saudi Arabia.
Awareness of Osteoporosis among 368 Residents in China: A Cross-Sectional Study
Authors: Kemal Oumer , Liu Yawen , Yu Qiong , Wu Fan , Yang Shuman 
Published: November 2019

Abstract
Background: Studies on osteoporosis awareness among general population in China are still limited. We examined the level of osteoporosis awareness among residents in China, determined the risk factors associated with lower level of osteoporosis awareness, and assessed the sources of their knowledge about osteoporosis. Methods: We conducted a cross-sectional study among 368 general residents aged 30 years or older from 19 provinces during January-March 2018 in China. All participants were identified and interviewed face-to-face by medical students in Jilin University using a structured questionnaire. Osteoporosis awareness scores (percent of correct answer) was determined across several domains, including definition, diagnosis, risk factors, and prevention of osteoporosis. We used multiple linear regression models to test the relationship between risk factors and overall awareness scores. Results: The mean age of participants was 52.9 ± 10.2 years, and 53% of them were male. Osteoporosis awareness score for definition was 77.7%, diagnosis 49.6%, risk factors 49.2%, treatment 60.5%, and prevention 69.9%. The overall awareness score was 67.8%. Lower family income and education level were significantly associated with lower overall awareness score (all p <0.05). Television or radio health program was reported to be their main source of knowledge about osteoporosis. Conclusion: The awareness level for osteoporosis is moderate; lower family income and education level were risk factors for lower awareness. Television or radio health program had the greatest contribution to osteoporosis awareness.