bluuebunny/crossref_metadata_2025_split huggingface dataset:
Alien introgression and morpho-agronomic characterization of diploid progenies of Solanum lycopersicoides monosomic alien addition lines (MAALs) toward pre-breeding applications in tomato (S. lycopersicum)
Authors: Mangat Puneet Kaur , Shim Junghyun , Gannaban Ritchel B. , Singleton Joshua J. , Angeles-Shim Rosalyn B. 
Published: January 2021

AbstractKey messageAlien introgressions that were captured in the genome of diploid plants segregating from progenies of monosomic alien addition lines ofS. lycopersicoidesconfer novel phenotypes with commercial and agronomic value in tomato breeding.AbstractSolanum lycopersicoidesis a wild relative of tomato with a natural adaptation to a wide array of biotic and abiotic challenges. In this study, we identified and characterized diploid plants segregating from the progenies of monosomic alien addition lines (MAALs) ofS. lycopersicoidesto establish their potential as donors in breeding for target trait improvement in tomato. Molecular genotyping identified 28 of 38 MAAL progenies having the complete chromosome complement of the cultivated tomato parent and limited chromosome introgressions from the wildS. lycopersicoidesparent. Analysis of SSR and indel marker profiles identified 34 unique alien introgressions in the 28 MAAL-derived introgression lines (MDILs) in the genetic background of tomato. Conserved patterns of alien introgressions were detected among sibs of MDILs 2, 3, 4 and 8. Across MDILs, a degree of preferential transmission of specific chromosome segments was also observed. Morphologically, the MDILs closely resembled the cultivated tomato more thanS. lycopersicoides. The appearance of novel phenotypes in the MDILs that are lacking in the cultivated parent or the source MAALs indicates the capture of novel genetic variation by the diploid introgression lines that can add commercial and agronomic value to tomato. In particular, screening of representative MDILs for drought tolerance at the vegetative stage identified MDIL 2 and MDIL 11III as drought tolerant based on visual scoring. A regulated increase in stomatal conductance of MDIL 2 under drought stress indicates better water use efficiency that allowed it to survive for 7 days under 0% moisture level.
Over-constrained kinematic of the medial compartment leads to lower clinical outcomes after total knee arthroplasty
Authors: Pizza Nicola , Di Paolo Stefano , Zinno Raffaele , Marcheggiani Muccioli Giulio Maria , Agostinone Piero , Alesi Domenico , Bontempi Marco , Zaffagnini Stefano , Bragonzoni Laura 
Published: January 2021

Abstract
Purpose
To investigate if postoperative clinical outcomes correlate with specific kinematic patterns after total knee arthroplasty (TKA) surgery. The hypothesis was that the group of patients with higher clinical outcomes would have shown postoperative medial pivot kinematics, while the group of patients with lower clinical outcomes would have not.

Methods
52 patients undergoing TKA surgery were prospectively evaluated at least a year of follow-up (13.5 ± 6.8 months) through clinical and functional Knee Society Score (KSS), and kinematically through dynamic radiostereometric analysis (RSA) during a sit-to-stand motor task. Patients received posterior-stabilized TKA design. Based on the result of the KSS, patients were divided into two groups: “KSS > 70 group”, patients with a good-to-excellent score (93.1 ± 6.8 points, n = 44); “KSS < 70 group”, patients with a fair-to-poor score (53.3 ± 18.3 points, n = 8). The anteroposterior (AP) low point (lowest femorotibial contact points) translation of medial and lateral femoral compartments was compared through Student’s t test (p < 0.05).

Results
Low point AP translation of the medial compartment was significantly lower (p < 0.05) than the lateral one in both the KSS > 70 (6.1 mm ± 4.4 mm vs 10.7 mm ± 4.6 mm) and the KSS < 70 groups (2.7 mm ± 3.5 mm vs 11.0 mm ± 5.6 mm). Furthermore, the AP translation of the lateral femoral compartment was not significantly different (p > 0.05) between the two groups, while the AP translation of the medial femoral compartment was significantly higher for the KSS > 70 group (p = 0.0442).

Conclusion
In the group of patients with a postoperative KSS < 70, the medial compartment translation was almost one-fourth of the lateral one. Surgeons should be aware that an over-constrained kinematic of the medial compartment might lead to lower clinical outcomes.

Level of evidence
II.
The effects of locations on the build tray on the quality of specimens in powder bed additive manufacturing
Authors: Pal Snehashis , Gubeljak Nenad , Bončina Tonica , Hudák Radovan , Toth Teodor , Zivcak Jozef , Lojen Gorazd , Leben Niko , Drstvenšek Igor 
Published: January 2021

AbstractIn this study, the effect of powder spreading direction was investigated on selectively laser-melted specimens. The results showed that the metallurgical properties of the specimens varied during fabrication with respect to their position on the build tray. The density, porosity, and tensile properties of the Co–Cr–W–Mo alloy were investigated on cuboid and tensile specimens fabricated at different locations. Two different significant positions on the tray were selected along the powder spreading direction. One set of specimens was located near the start line of powder spreading, and the other set was located near the end of the building tray. The main role in the consequences of powder layering was played by the distribution of powder particle sizes and the packing density of the layers. As a result, laser penetration, melt pool formation, and fusion characteristics varied. To confirm the occurrence of variations in sample density, an additional experiment was performed with a Ti–6Al–4V alloy. Furthermore, the powders were collected at two different fabricating locations and their size distribution for both materials was investigated.
Effects of holding soft objects during Cyberball tasks under frequent positive feedback
Authors: Ikeda Toshiki , Takeda Yuji 
Published: January 2021

AbstractA previous study suggested that holding soft objects enhanced expectations of uncertain events and increased social pain under frequent negative feedback; i.e., higher expectations might have induced more disappointment. The present study examined the effects of holding a soft cushion under frequent positive feedback. Participants (n = 42) performed fair-play and over-inclusion blocks in the Cyberball task. Amplitudes of the contingent negative variation (CNV) of event-related brain potentials and subjective ratings of social pain were measured to estimate participants’ expectations and emotions, respectively. CNV amplitudes were higher in the over-inclusion block when participants held the soft than the hard cushion. There was a statistically marginal trend (p = .095) for lower social pain scores in the soft cushion condition than the hard cushion condition in contrast to previous findings. These results suggest that holding a soft object does not directly modulate emotions but instead acts through the mediation of enhanced expectations.
The influence of recipient SLCO1B1 rs2291075 polymorphism on tacrolimus dose–corrected trough concentration in the early period after liver transplantation
Authors: Wu Yi , Fang Fang , Wang Zhaowen , Wen Peihao , Fan Junwei 
Published: January 2021

Abstract
Purpose
To explore the relationship between rs2291075 polymorphism in SLCO1B1 gene, which encodes an influx transmembrane protein transporter, and tacrolimus dose–corrected trough concentration (C/D, ng ml−1 mg−1 kg−1) in the early period after liver transplantation.

Methods
CYP3A5 rs776746 and SLCO1B1 rs2291075 polymorphisms of 210 liver transplantation patients and their corresponding donor livers were assessed by PCR amplification and DNA sequencing. The influence of gene polymorphisms on C/D values of tacrolimus was analyzed. The early postoperative period after liver transplantation was divided into the convalescence phase (1–14 days) and stationary phase (15–28 days) according to the change of liver function and tacrolimus C/D values.

Results
The combined analysis of donor and recipient CYP3A5 rs776746 could distinguish the metabolic phenotype of tacrolimus into three groups: fast elimination (FE), intermediate elimination (IE), and slow elimination (SE), which was entitled the FIS classification system. Tacrolimus C/D ratios of recipient SLCO1B1 rs2291075 CT and TT carriers were very close and were significantly lower than those of recipient SLCO1B1 rs2291075 CC genotype carriers in convalescence phase (p = 0.0195) and in stationary phase (p = 0.0152). There were no statistically significant differences between tacrolimus C/D ratios of patients carried with SLCO1B1 rs2291075 CT, TT genotype donors, and those carried with SLCO1B1 rs2291075 CC genotype donors. A model consisting of tacrolimus daily dose, total bilirubin, FIS classification, and recipient SLCO1B1 rs2291075 could predict tacrolimus C/D ratios in the convalescence phase by multivariate analysis. However, recipient SLCO1B1 rs2291075 genotype failed to enter forecast model for C/D ratios in stationary phase. Recipient SLCO1B1 rs2291075 genotype had significant effect on tacrolimus C/D ratios in convalescence phase (p = 0.0300) and stationary phase (p = 0.0400) in subgroup, which excluded the interference come from donor and recipient CYP3A5 rs776746.

Conclusion
SLCO1B1 rs2291075 could be a novel genetic locus associated with tacrolimus metabolism. The combined analysis of donor and recipient CYP3A5 rs776746, recipient SLCO1B1 rs2291075 genotypes, could be helpful to guide the personalized administration of tacrolimus in early period after liver transplantation.
Immune signature-based risk stratification and prediction of immune checkpoint inhibitor’s efficacy for lung adenocarcinoma
Authors: Yi Ming , Li Anping , Zhou Linghui , Chu Qian , Luo Suxia , Wu Kongming 
Published: January 2021

Abstract
Background
Lung adenocarcinoma (LUAD) is a common pulmonary malignant disease with a poor prognosis. There were limited studies investigating the influences of the tumor immune microenvironment on LUAD patients’ survival and response to immune checkpoint inhibitors (ICIs).

Methods
Based on TCGA-LUAD dataset, we constructed a prognostic immune signature and validated its predictive capability in the internal as well as total datasets. Then, we explored the differences of tumor-infiltrating lymphocytes, tumor mutation burden, and patients’ response to ICI treatment between the high-risk score group and low-risk score group.

Results
This immune signature consisted of 17 immune-related genes, which was an independent prognostic factor for LUAD patients. In the low-risk score group, patients had better overall survival. Although the differences were non-significant, patients with low-risk scores had more tumor-infiltrating follicular helper T cells and fewer macrophages (M0), which were closely related to clinical outcomes. Additionally, the total TMB was markedly decreased in the low-risk score group. Using immunophenoscore as a surrogate of ICI response, we found that patients with low-risk scores had significantly higher immunophenoscore.

Conclusion
The 17-immune-related genes signature may have prognostic and predictive relevance with ICI therapy but needs prospective validation.
Why compromise the patella? Five-year follow-up results of medial patellofemoral ligament reconstruction with soft tissue patellar fixation
Authors: Boelch Sebastian P. , Gurok Anna , Gilbert Fabian , Weißenberger Manuel , Rudert Maximilian , Barthel Thomas , Reppenhagen Stephan 
Published: January 2021

Abstract
Purpose
This study investigates the redislocation rate and functional outcome at a minimum follow-up of five years after medial patellofemoral ligament (MPFL) reconstruction with soft tissue patellar fixation for patella instability.

Methods
Patients were retrospectively identified and knees were evaluated for trochlea dysplasia according to Dejour, for presence of patella alta and for presence of cartilage lesion at surgery. At a minimum follow-up of five years, information about an incident of redislocation was obtained. Kujala, Lysholm, and Tegner questionnaires as well as range of motion were used to measure functional outcome.

Results
Eighty-nine knees were included. Follow-up rate for redislocation was 79.8% and for functional outcome 58.4%. After a mean follow-up of 5.8 years, the redislocation rate was 5.6%. There was significant improvement of the Kujala score (68.8 to 88.2, p = 0.000) and of the Lysholm score (71.3 to 88.4, p = 0.000). Range of motion at follow-up was 149.0° (115–165). 77.5% of the knees had patella alta and 52.9% trochlear dysplasia types B, C, or D. Patellar cartilage legions were present in 54.2%. Redislocations occurred in knees with trochlear dysplasia type C in combination with patella alta.

Conclusion
MPFL reconstruction with soft tissue patellar fixation leads to significant improvement of knee function and low midterm redislocation rate. Patients with high-grade trochlear dysplasia should be considered for additional osseous correction.
Influence of Northern Hemispheric Winter Warming on the Pacific Storm Track
Authors: Park Hyung-Ju , Kim Kwang-Yul 
Published: January 2021

AbstractEffect of global warming on the sub-seasonal variability of the Northern Hemispheric winter (NDJFM) Pacific storm-track (PST) activity has been investigated. Previous studies showed that the winter-averaged PST has shifted northward and intensified, which was explained in terms of energy exchange with the mean field. Effect of global warming exhibits spatio-temporal heterogeneity with predominance over the Arctic region and in the winter season. Therefore, seasonal averaging may hide important features on sub-seasonal scales. In this study, distinct sub-seasonal response in storm track activities to winter Northern Hemispheric warming is analyzed applying cyclostationary empirical orthogonal function analysis to ERA5 data. The key findings are as follows. Change in the PST is not uniform throughout the winter; the PST shifts northward in early winter (NDJ) and intensifies in late winter (FM). In early winter, the combined effect of weakened baroclinic process to the south of the climatological PST and weakened barotropic damping to the north is responsible for the northward shift. In late winter, both processes contribute to the amplification of the PST. Further, change in baroclinic energy conversion is quantitatively dominated by eddy heat flux, whereas axial tilting of eddies is primarily responsible for change in barotropic energy conversion. A close relationship between anomalous eddy heat flux and anomalous boundary heating, which is largely determined by surface turbulent heat flux, is also demonstrated.
The differing role of weather systems in southern Australian rainfall between 1979–1996 and 1997–2015
Authors: Pepler Acacia S. , Dowdy Andrew J. , Hope Pandora 
Published: January 2021

AbstractMost of the rainfall in southern Australia is associated with cyclones, cold fronts, and thunderstorms, and cases when these weather systems co-occur are particularly likely to cause extreme rainfall. Rainfall declines in some parts of southern Australia during the cool half of the year in recent decades have previously been attributed to decreases in the rainfall from fronts and/or cyclones, while thunderstorm-related rainfall has been observed to increase, particularly in the warm half of the year. However, the co-occurrence of these systems, particularly the co-occurrence of cyclones or fronts with thunderstorms, can be very important for rainfall in some areas, particularly heavy rainfall, and changes in the frequency of these combined weather systems have not been previously assessed. In this paper we show that the majority of the observed cool season rainfall decline between 1979–1996 and 1997–2015 in southeast Australia is associated with a decrease in the frequency of fronts and cyclones that produce rainfall, while there has simultaneously been an increase in the frequency of cold fronts and thunderstorms that do not produce rainfall in some regions. Thunderstorm rainfall has increased in much of southern Australia, particularly during the warm half of the year, including an increase in rainfall where a thunderstorm environment occurs at the same time as a cyclone or front.
Telemedicine in the pediatric surgery in Germany during the COVID-19 pandemic
Authors: Lakshin G. , Banek S. , Keese D. , Rolle U. , Schmedding A. 
Published: January 2021

Abstract
Purpose
The COVID-19 pandemic has led to an unprecedented expansion of telemedicine services worldwide. This study aimed to explore the practice of telemedicine in Pediatric Surgery in Germany, the impact of the pandemic on its development and parents’ and surgeons’ experiences with telemedicine.

Methods
The study is a cross-sectional analysis using three surveys between 6/2020 and 10/2020: (1) all Pediatric Surgery departments of Germany reported whether they provide telemedicine services. (2) Members of the German Society of Pediatric Surgery and (3) families who participated in an outpatient visit by telephone or video with the Department of Pediatric Surgery and Pediatric Urology of the University Hospital Frankfurt completed an anonymous survey on their experience with telemedicine.

Results
21% of the Pediatric Surgery departments in Germany provided telemedicine, of which 57% started due to the pandemic. The lack of physical examination and face-to-face contact seem to be the major limitations to surgeons and parents. 48% of the parents answered that telemedicine is equal to or better than traditional appointments, while 33% thought that telemedicine is worse.

Conclusions
This study shows that families and doctors alike have had positive experiences with telemedicine and most will continue to use this format after the pandemic.
Scientometric evaluation of highly cited scientists in the field of forensic science and legal medicine
Authors: Jones Alan Wayne 
Published: January 2021

AbstractA publically available database of the most highly cited scientists in all disciplines was used to identify people that belonged to the subject category “forensic science and legal medicine.” This bibliometric information was derived from Elsevier’s SCOPUS database containing eight million scientists with at least five articles as author or co-author. The top 100,000 most highly cited scientists were identified and ranked according to six citation metrics; total number of citations, H-index, H-index adjusted for co-authorship, citations to single-authored papers, citations to single or first author papers and, citations to single, first, or last-authored papers. The eight million entries in the SCOPUS database were sub-divided into 22 main subject categories and 176 sub-categories, one of which was legal and forensic medicine. The citation databases were provided as supplementary material in two articles published in PLoS Biology in 2019 and 2020. Among the top 100,000 most highly cited scientists, there were only 30 allocated to the legal and forensic medicine category, according to the 2019 PLoS Biology article. The updated database from 2020 also included the names of people within the top-cited 2% of their scientific discipline. This increased the number of forensic practitioners to 215 from a total of 10,158 individuals in this subject category. This article takes a closer look at these highly cited forensic scientists, the countries where they work, the particular research field in which they publish, and their composite citation scores with and without self-citations. The top ten most cited individuals in both databases (2019 and 2020) were the same and these should therefore be considered an elite group among all forensic practitioners.
Soluble αKlotho downregulates Orai1-mediated store-operated Ca2+ entry via PI3K-dependent signaling
Authors: Kim Ji-Hee , Park Eun Young , Hwang Kyu-Hee , Park Kyu-Sang , Choi Seong Jin , Cha Seung-Kuy 
Published: January 2021

AbstractαKlotho is a type 1 transmembrane anti-aging protein. αKlotho-deficient mice have premature aging phenotypes and an imbalance of ion homeostasis including Ca2+ and phosphate. Soluble αKlotho is known to regulate multiple ion channels and growth factor-mediated phosphoinositide-3-kinase (PI3K) signaling. Store-operated Ca2+ entry (SOCE) mediated by pore-forming subunit Orai1 and ER Ca2+ sensor STIM1 is a ubiquitous Ca2+ influx mechanism and has been implicated in multiple diseases. However, it is currently unknown whether soluble αKlotho regulates Orai1-mediated SOCE via PI3K-dependent signaling. Among the Klotho family, αKlotho downregulates SOCE while βKlotho or γKlotho does not affect SOCE. Soluble αKlotho suppresses serum-stimulated SOCE and Ca2+ release-activated Ca2+ (CRAC) channel currents. Serum increases the cell-surface abundance of Orai1 via stimulating vesicular exocytosis of the channel. The serum-stimulated SOCE and cell-surface abundance of Orai1 are inhibited by the preincubation of αKlotho protein or PI3K inhibitors. Moreover, the inhibition of SOCE and cell-surface abundance of Orai1 by pretreatment of brefeldin A or tetanus toxin or PI3K inhibitors prevents further inhibition by αKlotho. Functionally, we further show that soluble αKlotho ameliorates serum-stimulated SOCE and cell migration in breast and lung cancer cells. These results demonstrate that soluble αKlotho downregulates SOCE by inhibiting PI3K-driven vesicular exocytosis of the Orai1 channel and contributes to the suppression of SOCE-mediated tumor cell migration.
The role of clinical factors and immunocheckpoint molecules in the prognosis of patients with supratentorial extraventricular ependymoma: a single-center retrospective study
Authors: Wang Liguo , Han Song , Yan Changxiang , Yang Yakun , Li Zhiqiang , Yang Zuocheng 
Published: January 2021

Abstract
Purpose
Supratentorial extraventricular ependymoma (SEE) is a rare subset of ependymomas located in the supratentorial parenchyma, and little is known regarding its management and prognosis. Our study aimed to reveal the prognostic factors in patients with SEE and the roles of programmed death ligand-1 (PD-L1), programmed cell death protein 1 (PD-1), Ki-67, and neural cell adhesion molecule L1 (L1CAM) in predicting these patients’ outcomes.

Methods
We retrospectively studied the clinical features and prognostic factors in 48 patients with SEE admitted to our center from April 2008 to October 2018. Tissue slides were constructed from patient samples, and PD-L1, PD-1, Ki-67, and L1CAM expression levels were evaluated by immunohistochemistry.

Results
Patients with gross total resection (GTR) had better progression-free survival than patients with subtotal resection (STR). Moreover, the recurrence hazard ratios in patients with STR at 3, 5, and 10 years were 8.746, 6.866 and 3.962 times those of patients with GTR, respectively. PD-L1 positivity predicted worse progression-free survival, while the recurrence hazard ratios for patients with PD-L1 positivity at 3, 5, and 10 years were 10.445, 5.539, and 3.949 times those of patients with PD-L1 negativity, respectively. Multivariate analysis revealed that PD-L1 expression and GTR could independently predict outcomes in patients with SEE.

Conclusion
PD-L1 expression was an independent and more readily obtained predictor of outcomes, representing a simple and reliable biological prognostic factor for patients with SEE. Further studies are needed to explore PD-L1 inhibitor treatment for patients with ependymoma.

Clinical trial registration
No clinical trials were performed in the study.