bluuebunny/crossref_metadata_2025_split huggingface dataset:
Condiciones para el análisis de proteínas del micelio de Lentinula edodes obtenido por fermentación en estado líquido
Authors: Oliveros Carolina Vega , Chegwin Angarita Carolina , Ardila Barrantes Harold Dubán 
Published: November 2019

Lentinula edodes es una seta comestible con potencial para el desarrollo de nutraceúticos. Sin embargo, son incipientes los trabajos enfocados en su producción biotecnológica y el desarrollo de herramientas analíticas que permitan profundizar en su composición. En esta investigación se estudió la producción de biomasa del hongo en el tiempo mediante fermentación en estado líquido y se seleccionaron las condiciones que permiten la obtención de extractos para la aplicación de herramientas para análisis proteómicos. Los métodos de extracción de proteínas, ácido tricloroacético (TCA)-Acetona y TCA-Acetona-Fenol, fueron comparados en términos del rendimiento de extracción y los perfiles de separación usando electroforesis en 1D (SDS-PAGE) y 2D (IEF-SDS PAGE). Se determinó que a los 10 días de crecimiento se obtiene la mayor producción de biomasa y proteína total. La extracción con TCA-Acetona-Fenol presentó un mayor rendimiento, mayor resolución y número de bandas en la electroforesis 1D. En 2DE los dos métodos permitieron la extracción de proteínas con puntos isoeléctricos en el rango de pH 3-10, pero el método TCA-Acetona-Fenol conllevó a una extracción diferencial, favoreciendo el rango de 33 a 113 kDa. Estos resultados se constituyen en una primera aplicación de técnicas de separación electroforética para futuros estudios proteómicos
Factors Affecting Risk Disclosure in Listed Indonesian Consumer Goods Industry Companies
Authors: Sabrina Sasya , Yessy Yessy 
Published: November 2019

<p>The demand for information disclosure by investor is increasing especially the disclosure related to non-financial information since investment activity is an activity that contains risks and uncertainties. The purpose of this research is to analyze the effect of foreign share ownership, public share ownership, profitability, and company size on risk disclosure. The agency theory and the stakeholder theory are used as the supporting theory in this research. This research population is listed consumer goods industry companies in 2013-2015. The data were selected using purposive sampling. The 75 data from 25 companies were collected and analysed using multiple linear regressions. The results of this research are foreign share ownership has significant effect on risk disclosure, firm size has significant effect on risk disclosure, public share ownership has no significant effect on risk disclosure, and profitability has no significant effect on risk disclosure. This research is expected to be able to contribute to provide a more understanding of the importance of risk disclosure and the important factors affecting the risk disclosure.</p><p><em><strong>Abstrak dalam Bahasa Indonesia</strong> : Kebutuhan investor atas pengungkapan informasi meningkat terutama pengungkapan yang terkait informasi non keuangan karena aktivitas investasi merupakan aktivitas yang mengandung risiko dan ketidakpastian. Tujuan dari penelitian ini adalah untuk menganalisis pengaruh kepemilikan saham asing, kepemilikan saham publik, profitabilitas, and ukuran perusahaan pada pengungkapan risiko. Teori keagenan dan teori pemangku kepentingan digunakan sebagai teori pendukung pada penelitian ini. Penelitian ini menggunakan populasi perusahaan industri barang konsumsi yang terdaftar pada Bursa Efek Indonesia pada tahun 2013-2015. Data dipilih menggunakan purposive sampling. 75 data dari 25 perusahaan industri barang konsumsi dikumpulkan dan dianalisis dengan regresi linear berganda. Penelitian ini memberikan hasil sebagai berikut: kepemilikan saham asing mempunyai pengaruh signifikan pada pengungkapan risiko, ukuran perusahaan mempunyai pengaruh signifikan pada pengungkapan risiko, kepemilikan saham publik tidak mempunyai pengaruh signifikan pada pengungkapan risiko, dan profitabiltas tidak mempunyai pengaruh signifikan pada pengungkapan risiko. Penelitian ini diharapkan dapat berkontribusi memberikan pemahaman yang lebih banyak atas pentingnya pengungkapan risiko dan faktor penting yang mempengaruhi pengungkapan risiko.</em></p>
O ethos como índice de virtude discursiva
Authors: Vilela-Ardenghi Ana Carolina , Gomes Cristiana Frazão 
Published: November 2019

Paveau (2013a) apresenta uma proposta que é, pode-se dizer, inovadora, na medida em que pretende dotar a Análise do Discurso de uma dimensão cognitiva. Inspirado aí, o presente artigo objetiva — a partir da análise de uma campanha da cerveja Skol para o carnaval de 2015 — testar a produtividade da incorporação de tal dimensão ao ethos. Para tanto, buscamos apoio também na possibilidade de se incorporar o estudo da moral na linguagem, conforme Paveau (2015), que argumenta que há enunciados reconhecidos, no seio de uma dada comunidade, como virtuosos ou não. Assim, os membros dessa comunidade sabem, através de dados distribuídos socioculturalmente no ambiente, e têm (ou deveriam ter) uma disposição para produzir enunciados virtuosos. A essa disposição a autora designa virtude discursiva. Defendemos aqui que o ethosé um índice dessa virtude e que pode, portanto, ter incorporado em sua definição uma dimensão cognitiva, para além de ser regrado, do ponto de vista estritamente enunciativo-discursivo, por uma semântica global (MAINGUENEAU, 2005).
Ideologi Gender dalam Konstruksi Kurikulum Program Studi di Universitas Negeri Semarang
Authors: Puspitarini Yanuari Dwi , Utanto Yuli 
Published: November 2019

The purpose of this study is to find out how far the gender ideology influence students in choosing courses and how the curriculum construction which is developed by Early Childhood Education and Physical, Sports and Recreation teacher program. This research is a qualitative research with phenomenological approach. This research was conducted at the teacher education of three programs (early childhood education, physical, sports and recreation program) in Universitas Negeri Semarang. The subjects of this research are the students and the lecturers of each study program. The technique of data collection in this research used an interview technique. The results of this research are gender ideology did not directly affect the students in choosing a specific study program, but it begins from the parenting system that is applied by the parents. The structure of curriculum in each study program is arranged in appropriate with the demands in the employment industry
A Review of Bundled Payments in Total Joint Replacement
Authors: Manickas-Hill Olivia , Feeley Thomas , Bozic Kevin J. 
Published: November 2019

» The Bundled Payments for Care Improvement (BPCI) initiative, developed by the U.S. Center for Medicare & Medicaid Innovation, aims to reduce health-care expenditures while maintaining or improving patient outcomes.» Several published reports evaluating the impact of the BPCI on payment, utilization, and patient outcomes during the first 3 years of the initiative demonstrated a reduction in Medicare payments for major joint replacement of the lower extremity, driven primarily by a reduction in post-acute care utilization, without a sacrifice in quality of care as measured by no change in unplanned readmission rate, emergency department use, or mortality rate during the 90 days from episode start date. However, this reduction in Medicare payments did not translate into savings to the Medicare program, as the Net Payment Reconciliation Amount (NPRA) to providers was not included in any of the cost analyses.» The impact of the BPCI on outcomes important to patients and physicians has not been thoroughly evaluated in the published literature. Important (and standardly trackable) outcome measures that can be considered when assessing the effectiveness of a health-care model for bundled payments for total joint replacement include 30-day postoperative mortality, reoperation, and readmission; hip or knee functional status; hip, knee, or lower back pain; quality of life; work status; and satisfaction with results. Many studies directly evaluated only survival and disutility as manifested by readmission rate and emergency department use.» Multiple pilot studies evaluating cost savings and quality measures using a bundled-payment alternative payment model have demonstrated a reduction in Medicare episode payments, primarily related to decreased length of hospital stay and post-acute care utilization.» Readmission, certain comorbidities, and low socioeconomic status are associated with higher episode costs.» An evaluation of a bundled payment program for orthopaedic procedures in Stockholm, Sweden, revealed a decrease in cost, complication rate, and wait time after the introduction of a bundled payment system. Some of these improvements may be related to an increase in the number of surgical procedures on younger, healthier patients performed at specialized orthopaedic centers, with a concomitant shift of patients with comorbidities to hospitals.
Frecuencia del cuarto conducto y variaciones anatómicas en segundas y terceras molares superiores mediante tomografía computarizada de haz cónico
Authors: Calero-Hinostroza Gisela , Gonzales-Gonzales Ronald , García-Rupaya Carmen Rosa 
Published: November 2019

Objetivo: Evaluar la frecuencia del cuarto conducto y variaciones anatómicas en segundas y terceras molares superiores mediante tomografía de haz cónico volumétrico.Materiales y método: Se incluyeron segundos o terceros molares superiores de pacientes en 120 tomografías de haz cónico volumétrico, recolectadas entre enero y diciembre de 2016. Las tomografías se obtuvieron con un tomógrafo Point 3D Combi 500 S con sensor de FOV de 12 x 9 cm. Se realizó el análisis mediante SPSS y se realizó la prueba de Chi-cuadrado de Pearson.Resultados: La frecuencia del cuarto conducto de las segundas molares superiores fue de 40% y en terceras molares superiores fue de 4,8%. La variante tipo 8 fue de 47% y la variante 9 fue de 38,8% en segundas molares superiores. La variante 3 fue de 34,9% y la variante tipo 1 fue de 28,1% en terceras molares superiores. La mayor frecuencia fue de 3 raíces, 86,5% en segundas molares superiores y en las terceras molares superiores 69,2% tuvo una sola raíz. La bilateralidad en segundas molares superiores fue de 69,4% y en terceras molares superiores fue de 69,9%. Conclusiones: En segundas molares superiores la presencia del cuarto conducto fue de 40% y en las terceras molares superiores fue 4,8%. Palabras clave: cuarto conducto, tomografía de haz cónico volumétrico, segunda molar superior, tercera molar superior”
Analysis of factors influencing hospital-acquired infection in postoperative patients with intracranial aneurysm
Authors: Wang Jun , Ji Yuanyuan , Jiang Lidan , Zhao Xia , Guan Shaochen , Yang Piao , Yu Jie , Liu Yunyun , Zhang Hongqi 
Published: November 2019

Abstract
BackgroundHospital-acquired infection (HAI) is a serious complication of neurosurgery. In recent years, the medical body has paid increasing attention to this issue. Aim We investigated the status of HAIs in patients who had undergone surgery for intracranial aneurysms and analysed their risk factors. Methods A retrospective analysis was carried out on the medical records of 542 patients with intracranial aneurysms after they were admitted for neurosurgery at Xuanwu Hospital of Capital Medical University between January and December 2016. Cases studied were divided into an infection group and a control group.Logistic regression analysis of the data was carried out. Findings Of the 542 patients with intracranial aneurysms who underwent surgery, 77 HAIs occurred in 64 patients, with an infection prevalence of 11.8% and prevalence of infection cases of 14.2%. Logistic regression showed that an admission Glasgow Coma Scale (GCS) score of less than 8 points (odds ratio= 4.261, 95% confidence interval 1.102–16.476), hyperglycaemia (2.759, 1.159–6.564), hypothermia treatment (6.557,2.244–19.159), and central venous catheterisation (CVC) (8.853, 2.860–27.398) were independent risk factors for HAIs in patients with intracranial aneurysm who underwent surgery. ConclusionBeing comatose upon hospital admission, having hyperglycaemia or hypothermia, and indwelling CVC are major risk factors for HAIs in patients undergoing surgery for intracranial aneurysms.
Effect of airway administration of budesonide for bronchopulmonary dysplasia in premature infants: A Systematic Review and Meta-Analysis
Authors: wang kaixu , Chen Long , Li Fang 
Published: November 2019

Abstract
Objective: Bronchopulmonary dysplasia (BPD) is one of the major challenges in preterm infants despite the therapeutic improvement. Airway administration of budesonide might be a safe and effective way. However, the optimal timing of airway administration is under determined. The meta-analysis was designed to evaluate the effectiveness and safety of early (≤1d after birth) and late (>1d after birth) airway administration of budesonide in decreasing the incidence of BPD and death as the primary outcome . Methods: PubMed, EMBASE, the Cochrane Library, China national knowledge internet (CNKI), China biology medicine disc (CBM), WANFANG data, and China Science and Technology Journal Database were searched for RCTs that compared airway administration of budesonide with controls. The meta-analysis was performed using Review Manager 5.3. Results: Airway administration of budesonide decreased the risk of BPD at 36 weeks PMA and the composite outcome of BPD or death (RR=0.64, 95%CI: 0.55~0.75 and RR=0.71, 95%CI: 0.57~0.89).Moreover, 37% and 36% reduction was observed in the incidence of BPD and the composite outcome of BPD or death in the early airway administration group (≤1d) (RR=0.63, 95%CI: 0.53~0.75 and RR=0.64, 95%CI: 0.47~0.87 ), while no difference was found in late airway administration group (>1d) (RR=0.74, 95%CI: 0.49~1.13 and RR=0.88, 95%CI: 0.64~1.21). Conclusion: early airway administration (≤1d) of budesonide reduced the incidence of BPD alone or composite outcome of death or BPD, and it is safe without increasing death as well as other short-term side effects. However, because of the small number of infants in late airway administration group and lacking of long-term follow-up, more randomized controlled trials are needed to testify for the outcomes. Keywords: Budesonide, bronchopulmonary dysplasia, premature infants, meta-analysis, airway administration.
Dentists’ knowledge and practices about infective endocarditis antibiotics prophylaxis among children in southwestern Saudi Arabia
Authors: Shati Ayed A 
Published: November 2019

Abstract
Background Infective endocarditis is a disease endangering the life of humans. Therefore, several prophylactic measures are needed to improve the protection of endocarditis-prone patients from bacteremia resulting from various dental actions. These measures range from developing the dental hygiene of endocarditis-prone individuals to trials of different antimicrobial agents. The objective of the study was to examine knowledge and practices of dentists in the Aseer Region, Saudi Arabia, regarding antibiotic prophylaxis against Infective endocarditis.Methods A cross-sectional study design in Aseer Region. Interviews were carried out, and 182 individuals participated in the data collection process. The 182 participants were interviewed at their workplaces. The data collection sheet was constructed based on the guidelines of the American Heart Association /American College of Cardiology (AHA/ACC, 2017).Results Dentists’ mean knowledge score was 17.5±3.7 (out of 24). The least correct responses regarding dental procedures that require prophylactic antibiotics were “root canal treatment” (30.8%). Regarding cardiac conditions that require prophylactic antibiotics, dentists’ least correct responses were “heart failure” (50%). Dentists’ mean knowledge scores differed significantly according to their age groups (p=0.032), nationality (p=0.002), education/qualification (p=0.002). Mean knowledge scores differed significantly according to dentists’ years of operation (p=0.018) and sources of information (p<0.001). Amoxicillin was the most regularly recommended antibiotic (90.7%), while 86.8% correctly stated 30-60 minutes before the procedure as the time for prophylactic antibiotic administration.Conclusions Knowledge of dentists in the Aseer Region regarding the use of preventive drugs for the control and prevention of I.E. is suboptimal. The inclusion of latest AHA guidelines into the dentistry curricula is highly recommended.
The combination therapy of steroids, tacrolimus and mycophenolate mofetil in idiopathic membranous nephropathy coexisting with type 2 diabetes mellitus: a retrospective study
Authors: Wang Yinhong , Tian Xuefei , Wang Hao , Ren Peiyao , Yan Gaofei , Wang Li , Ma Xiaotao , Ge Heng , Jia Lining , Fu Ronguo 
Published: November 2019

Abstract
Background: Idiopathic membranous nephropathy (IMN), one of the most common causes resulting into nephrotic syndrome in adults, is usually clinically treated with steroids and/or other immunosuppressive agents. However, the treatment on the patients with IMN coexisting with type 2 diabetes mellitus (DM) is still challenging. There are few convincing literatures available on the efficacy and safety of therapeutic regimens in this subgroup of patients to guide clinical practice. Methods: We retrospectively collected and analyzed the data of twenty-three patients who underwent renal biopsies between January 2013 and March 2018 in our institute and were diagnosed with IMN coexisting with type 2 DM. Nine patients received prednisone combining with tacrolimus and mycophenolate mofetil (group 1), while 14 patients received prednisone and intravenous cyclophosphamide (group 2). The primary endpoint was measured by percentage of patients achieved complete remission (CR) or partial remission (PR), the secondary endpoints included changes of urinary protein excretion from baseline and treatment-related adverse events. Results: The remission rates were compared between the group 1 and the group 2 at both 6 months (44.4% versus 50%, P>0.05 ) and 12 months (77.8% versus 64.3%, P>0.05 ). Significant decrease in urinary protein excretion was observed in both groups during treatment, which was significantly greater in group 1 than that in group 2. No significant renal impairment was observed during the follow-up period. Infections and worsening glycemic control were the most common adverse effects of two therapy. Patients treated with prednisone plus cyclophosphamide were more likely to develop worsening diabetes, while infections tended to be more common in patients treated with prednisone combining with tacrolimus and mycophenolate mofetil. Conclusions: The combination therapy of prednisone, tacrolimus and mycophenolate mofetil is one of the effective and safe options for patients with IMN and type 2 DM.
Biophysical Characterization of Interaction between E. coli Alanyl-tRNA Synethase with its Promoter DNA
Authors: Banerjee Baisakhi , Ganguli Sayak , Banerjee Rajat 
Published: November 2019

Background:Aminoacyl-tRNA Synthetases (aaRSs) are well known for their role in the translation process. Lately investigators have discovered that this family of enzymes are also capable of executing a broad repertoire of functions that not only impact protein synthesis, but extend to a number of other activities. Till date, transcriptional regulation has so far only been described in E. coli Alanyl-tRNA synthetase and it was demonstrated that alaRS binds specifically to the palindromic DNA sequence flanking the gene’s transcriptional start site and thereby regulating its own transcription.Objective:In the present study, we have characterized some of the features of the alaRS-DNA binding using various biophysical techniques.Methods:To understand the role of full length protein and oligomerization of alaRS in promoter DNA binding, two mutants were constructed, namely, N700 (a monomer, containing the N-terminal aminoacylation domain but without the C-terminal part) and G674D (previously demonstrated to form full-length monomer). Protein-DNA binding study using fluorescence spectroscopy, analytical ultracentrifugation, Isothermal Titration Calorimetry was conducted.Results:Sedimentation equilibrium studies clearly demonstrated that monomeric variants were unable to bind promoter DNA. Isothermal Calorimetry (ITC) experiment was employed for further characterization of wild type alaRS-DNA interaction. It was observed that full length E. coli Alanyl-tRNA synthetase binds specifically with its promoter DNA and forms a dimer of dimers. On the other hand the two mutant variants were unable to bind with the DNA.Conclusion:In this study it was concluded that full length E. coli Alanyl-tRNA synthetase undergoes a conformational change in presence of its promoter DNA leading to formation of higher order structures. However, the exact mechanism behind this binding is currently unknown and beyond the scope of this study.
Parkinson’s Disease: A Review from Pathophysiology to Treatment
Authors: Marino Bianca L.B. , de Souza Lucilene R. , Sousa Kessia P.A. , Ferreira Jaderson V. , Padilha Elias C. , da Silva Carlos H.T.P. , Taft Carlton A. , Hage-Melim Lorane I.S. 
Published: November 2019

:Parkinson's Disease (PD) is the second most common neurodegenerative disease in the elderly population, with a higher prevalence in men, independent of race and social class; it affects approximately 1.5 to 2.0% of the elderly population over 60 years and 4% for those over 80 years of age. PD is caused by the necrosis of dopaminergic neurons in the substantia nigra, which is the brain region responsible for the synthesis of the neurotransmitter dopamine (DA), resulting in its decrease in the synaptic cleft. The monoamine oxidase B (MAO-B) degrades dopamine, promoting the glutamate accumulation and oxidative stress with the release of free radicals, causing excitotoxicity. The PD symptoms are progressive physical limitations such as rigidity, bradykinesia, tremor, postural instability and disability in functional performance. Considering that there are no laboratory tests, biomarkers or imaging studies to confirm the disease, the diagnosis of PD is made by analyzing the motor features. There is no cure for PD, and the pharmacological treatment consists of a dopaminergic supplement with levodopa, COMT inhibitors, anticholinergics agents, dopaminergic agonists, and inhibitors of MAO-B, which basically aims to control the symptoms, enabling better functional mobility and increasing life expectancy of the treated PD patients. Due to the importance and increasing prevalence of PD in the world, this study reviews information on the pathophysiology, symptomatology as well as the most current and relevant treatments of PD patients.