bluuebunny/crossref_metadata_2025_split huggingface dataset:
A broad-range survey of Borrelia burgdorferi sensu lato infection in small mammals in Sino-Burmese border area, Yunnan Province, China
Authors: He Zhihai , Jiang Baogui , Gao Zihou , Shao Zongti , Zhang Yun , Liu Zhengxiang , Li Yuqiong , Pu Ennian , Tang Li , Yao Mingguo , Jia Na , Fricken Michael E. von , Jiang Jiafu , Cao Wuchun , Du Chunhong 
Published: March 2020

Abstract
Background: Lyme disease is caused by Borrelia burgdorferi sensu lato which is usually found in wild and domestic mammals worldwide. In China, Human cases of B. burgdorferi infections have been identified in a wide distribution, but little direct surveillance of potential rodent reservoirs has been performed in Yunnan Province, the tropical region, Sino-Burmese border area, southwestern China. Here we report a thoroughly investigation of B. burgdorferi sensu lato in small mammals collected in 2011 to 2016 in the region.Methods: A nested PCR was done for the 5S-23S rRNA intergenic spacer gene of Borrelia burgdorferi sensu lato. The PCR-positive amplicons were directly sequenced. Sequence analysis was carried out using a FASTA search on the Genbank database, with phylogenetic trees constructed using MEGA software, version 6.06. Statistical Analysis were conducted using SPSS.version 17.0.Results: Totally 57 species, 3659 mammals were captured at 159 sample sites located in 23 counties in Yunnan Province. Thirty species, 146 (3.99%) mammals were tested positive for B. bourgdorferi s.l . by nested PCR based on 5S-23S rRNA intergenic spacer gene. 20 species mammals were first reported infected with B. burgdorferi s.l. Sequence analysis revealed that five genotypes of B. burgdorferi s.l. were detected, including B. afzelii , B. burgdorferi sensu stricto, B. japonica , B. garinii and B. valaisiana.Conclusions: Significant differences in prevalence rates of B. bourgdorferi s.l were observed at varying landscape types and altitudes. Small mammals in forested areas had higher prevalence rates than other landscape types as did small mammals found at altitudes greater than 2500 meters. The 5S-23S rRNA intergenic spacer gene revealed that there were five genotypes of B. burgdorferi s.l. detected, indicating high genetic diversity within this province.
Prognostic value of neuron specific enolase in patients with advanced and metastatic non-neuroendocrine non-small cell lung cancer
Authors: Yan Peng , Han Yi-ming , Liu Chuan-yong , Liu Jie , Zuo Tai-yang , Wang Xin-yu 
Published: March 2020

Abstract
Background: Increased serum NSE levels were found in a substantial proportion (30%-69%) of patients with NSCLC, but little is known about the clinical properties of neuron specific enolase (NSE). Objective: We aimed to access the level of serum NSE to predict the prognosis and treatment response in advanced or metastatic non-neuroendocrine non-small cell lung cancer. Methods: We retrospectively analyzed 363 patients with advanced and metastatic NSCLC (without neuroendocrine immunohistochemistry stain and brain metastasis) between January 2011 and October 2016. The serum NSE level was measured before initiation of treatment. Results: According to the X-tile program, the optimum cut-off value for NSE level was 26.1 ng/ml. Patients with elevated NSE level (≥26.1 ng/ml) showed significantly shorter progression-free survival (PFS) (median PFS, 4.31 months vs 9.93 months; P < 0.001) and significantly shorter overall survival (OS) (median OS, 8.13 months vs 17.11 months; P < 0.001). In subgroup analysis, patients with elevated NSE level (≥26.1 ng/ml) showed significantly shorter PFS than those with low NSE level (<26.1ng/ml) in EGFR mutations group and EGFR-wide type group (6.58 months vs 16.02 months, P = 0.0016; 3.42 months vs 7.46 months, P < 0.001, respectively). In terms of OS, the patients with elevated NSE was also significantly shorter than those with low NSE in EGFR mutations group and EGFR-wide type group (9.51 months vs 27.73 months, P < 0.001; 7.76 months vs 13.55 months, P < 0.001, respectively). Univariate and multivariate analysis demonstrated that elevated NSE level was independent prognostic factors for short PFS (hazard ratio [HR] = 2.40 (1.71-3.38), P < 0.001; HR = 1.81 (1.28-2.56), P = 0.001, respectively) and OS (HR, 2.40 (1.71-3.37), P < 0.001; HR, 1.76 (1.24-2.50), P = 0.002, respectively). Conclusion: The survival of NSCLC with elevated serum NSE level was shorter than that of NSCLC with low NSE level. Serum NSE level is a predictor of treatment and independent prognostic factor.
Meta-analysis based gene expressional profiling reveals functional genes in ovarian cancer
Authors: Zhao Lin , Li Yuhui , Zhang Zhen , Guo Hong , Li Jianfu , Wei Ran , Guo Qiang , Zhu Xiaoxiao , Chu Chu , Zhang Yunhong , Wang Bin , Yue Jinbo , Li Xia 
Published: March 2020

Abstract
Background Ovarian cancer causes high mortality rate worldwide, and despite numerous attempts, the outcome for patients with ovarian cancer are still not well improved. Microarray based gene expressional analysis provides with valuable information for discriminating functional genes in ovarian cancer development and progression. However, due to the differences in experimental design, the results varied significantly across individual datasets. Methods In the present study, the data of gene expression in ovarian cancer was downloaded from Gene Expression Omnibus and 16 studies were included. A meta-analysis based gene expression analysis was performed to identify differentially expressed genes (DEGs). The most differentially expressed genes in our meta-analysis were selected for gene expression and gene function validation. Results A total of 972 DEGs with P -value<0.001 were identified in ovarian cancer, including 541 up-regulated genes and 431 down-regulated genes, among which 92 additional DEGs were found as gained DEGs. Top five up-and down-regulated genes were selected for the validation of gene expressional profiling. Among these genes, up-regulated CD24 , SOX17 , WFDC2 , EPCAM , INAVA , and down-regulated AOX1 were revealed to be with consistent expressional patterns in clinical patient samples of ovarian cancer. Gene functional analysis demonstrated that up-regulated WFCD2 and INAVA promoted ovarian cancer cell migration, WFDC2 enhanced cell proliferation, while down-regulated AOX1 was functional in inducing cell apoptosis of ovarian cancer. Interestingly. Conclusion Our study shed light on the molecular mechanisms underlying the development of ovarian cancer, and facilitated the understanding of novel diagnostic and therapeutic targets in ovarian cancer.
Prognostic factors for the short-term mortality of patients with rheumatoid arthritis admitted to intensive care units
Authors: Fujiwara Toshifumi , Tokuda Kentaro , Momii Kenta , Tsushima Hidetoshi , Akasaki Yukio , Ikemura Satoshi , Fukushi Jun-ichi , Maki Jun , Kaku Noriyuki , Akahoshi Tomohiko , Taguchi Tomoaki , Nakashima Yasuharu 
Published: March 2020

Abstract
Background Patients with rheumatoid arthritis (RA) have high mortality risk and are frequently treated in intensive care units (ICUs).Materials and Methods This study included 67 patients (20 males, 47 females) with RA who were admitted at the ICU of our institution for ≥48 h between January 2008 and March 2018. We analyzed the 30-day mortality of these patients and the investigated prognostic factors in RA patients admitted to our ICU.Results Upon admission, the mean age was 68 years, and RA duration was 14 years. The 30-day, 90-day, and 1-year mortality rates were 22%, 27%, and 37%, respectively. The major reasons for ICU admission were cardiovascular complications (24%) and infection (40%) and the most common ICU treatments were mechanical ventilation (69%), renal replacement (25%), and vasopressor (78%). In the 30-day mortality group, infection led to a fatal outcome in most cases (67%), and nonsurvivors were associated with a significantly higher prednisone dose, Charlson’s comorbidity index, and acute physiology and chronic health evaluation (APACHE) II score. Blood data at ICU admission showed that lower platelet number and total protein and higher creatinine and prothrombin time international normalized ratio (PT-INR) indicated significantly poorer prognosis. Multivariate analysis revealed that dose of prednisone, high APACHE II score, and prolonged PT-INR indicated a higher risk of 30-day mortality.Conclusion Our study revealed that high prednisone dose, elevated APACHE II score, and coagulation abnormalities predicted poorer prognosis in RA patients admitted to the ICU.
The emergence of the national medical assistance scheme for the poorest in Mali
Authors: Touré Laurence , Ridde Valery 
Published: March 2020

Abstract
Background : Universal health coverage (UHC) is now high up the international agenda. There are still major needs to be met in West Africa, particularly in Mali, where providing health care for the poorest remains a big challenge. The majority of the region’s countries are currently seeking to define the content of their compulsory, contribution-based medical insurance system. However, very few countries apart from Mali have decided to, in parallel, develop a solution for poorest that is not based on contributions. Methods : This qualitative research article examines the historical process that has permitted the emergence of this ground-breaking public policy. Results : The research shows that the process has been very long, chaotic and sometimes suspended for long periods. One of the biggest challenges has been that of intersectoriality and the social construction of the groups to be targeted by this public policy (the poorest), as institutional tensions have evolved in accordance with the political issues linked to social protection. Eventually, the medical assistance scheme for the poorest (RAMED) saw the light of day in 2011, funded entirely by the government. Conclusions : RAMED emergence would appear to be attributable not so much to any new concern for the poorest in society but rather to a desire to give the social protection policy engaged in a guarantee of universality. The RAMED nonetheless remains an innovation within French-speaking West Africa.
Adherence patterns among patients receiving healthcare services in the Muhimbili Sickle Cell Cohort, Tanzania
Authors: Masamu Upendo , Sangeda Raphael Zozimus , Kandoga Daniel , Ondego Jesca , Ndobho Florah , Mmbando Bruno , Nkya Siana , Msami Khadija , Makani Julie 
Published: March 2020

Abstract
Background: Monitoring of patient’s clinical attendance is one of the crucial means that is used to improve adherence to care and treatment among the Sickle Cell Disease (SCD) patients. Adherence to care has been shown to improve health outcomes in SCD patients. However, these benefits cannot be achieved when patients are lost to follow-up to care. Method : We analyzed data on loss to follow up to determine the patterns among sickle cell patients registered at Muhimbili Sickle Cell Cohort (MSC), in Dar es Salaam, Tanzania. Data was aggregated and analysed using R software and Microsoft Excel Spreadsheet. Survival analysis techniques, both non-parametric methods (Kaplan-Meier estimator and Log-rank test) and semi-parametric method (Cox’s proportional hazard model), were used. A p-value of 0.05 was considered significant to make a strong inference of the analysis. Results: 5476 SCD patients were registered at MSC from 2004 to 2016, 3350 (58.13%) were actively participating in clinics while, 2126 (41.87%) were inactive, out of which 35.19% were lost to follow-up. From the survival analysis results, patients who were between 5 to 17 years were more likely to be lost to follow-up than the rest with a hazard ratio of 2.65 times more than those who were above 18 years. Patients with mean cell volume above 77.73 fL and white blood cell above 15.73(10ˆ3/uL) were more likely to be loss to follow-up than those below average. Conclusion: Loss to follow-up is evident in a cohort of patients in long term comprehensive care follow-up. It is, therefore, necessary to design interventions that minimize its impacts. Suggested solutions might include training of the health care workers, more emphasis on newborn screening and advocacy to patients regarding the effect of loss to follow-up.
The agonist of JWA gene JAC1 suppresses proliferation of breast cancer through JWA/p38/SMURF1/HER2 signaling
Authors: Ren Yanlin , Chen Dongyin , Chen Junjie , Zhai Zurong , Li Aiping , Liang Yan , Yin Yongmei , Shu Yongqian , Zhou Jianwei 
Published: March 2020

Abstract
Background The HER2-positive breast cancer accounts for 20-30% of all breast cancers. The overexpression of HER2 is associated with malignant proliferation and invasiveness in several cancers. Some HER2 targeting drugs are widely used in clinic to block HER2 or its tyrosine kinase and improve the prognosis of patients. However, drug resistance and side effects are still a big challenge worldwide. Our previous studies have demonstrated that JWA down-regulates HER2 expression and inhibits cell proliferation and migration in gastric cancer cells. Methods The association of JWA with HER2 were analyzed by IHC in 90 tumor sample tissues and paired non-cancerous adjacent normal tissues. The impact of JAC1 on HER2-positive breast cancer cells was studied using colony formation assay. The effect of JAC1 on the localization of HER2 was detected by immunofluorescence microscopy assay. Western blotting, RT-PCR and immunoprecipitation assay were utilized to investigate the mechanisms of JWA regulating HER2. Finally, xenograft mouse models using BT474 cells were established to confirm the effect of JAC1 on tumor growth. Results In this study, we found that JAC1, a small molecule agonist of JWA gene, dose-dependently suppressed HER2-positive breast cancer proliferation in vitro and in vivo . Importantly, the inhibitory roles of JAC1 was due to the degradation of HER2 overexpression. The mechanistic evidences showed JAC1 increased the ubiquitination of HER2 at the K716 through the E3 ubiquitin ligase SMURF1; further, the activation of SMURF1 was due to reduced expression of NEDD4, an E3 ubiquitin ligase for SMURF1. Finally, we identified that the role of JAC1 on NEDD4 was realized through JWA-p38-GATA-1-NEDD4 axis. Conclusions These findings suggest that JAC1 may as a novel therapeutic agent to breast cancer through JWA/p38/GATA-1/NEDD4/SMURF1/HER2 signaling axis.
Proteogenomic analysis of Inhibitor of Differentiation 4 (ID4) in basal-like breast cancer
Authors: Baker Laura A. , Holliday Holly , Roden Daniel , Krisp Christoph , Wu Sunny Z. , Junankar Simon , Serandour Aurelien A. , Mohammed Hisham , Nair Radhika , Sankaranarayanan Geetha , Law Andrew M.K. , McFarland Andrea , Simpson Peter T. , Lakhani Sunil , Dodson Eoin , Selinger Christina , Anderson Lyndal , Samimi Goli , Hacker Neville F. , Lim Elgene , Ormandy Christopher J. , Naylor Matthew J. , Simpson Kaylene , Nikolic Iva , O’Toole Sandra , Kaplan Warren , Cowley Mark J. , Carroll Jason S. , Molloy Mark , Swarbrick Alexander 
Published: March 2020

Abstract
Background Basal-like breast cancer (BLBC) is a poorly characterised, heterogeneous disease. Patients are diagnosed with aggressive, high-grade tumours and often relapse with chemotherapy resistance. Detailed understanding of the molecular underpinnings of this disease is essential to the development of personalised therapeutic strategies. Inhibitor of Differentiation 4 (ID4) is a helix-loop-helix transcriptional regulator required for mammary gland development. ID4 is overexpressed in a subset of BLBC patients, associating with a stem-like poor prognosis phenotype, and is necessary for the growth of cell line models of BLBC, through unknown mechanisms. Methods Here, we have defined unique molecular insights into the function of ID4 in BLBC and the related disease high-grade serous ovarian cancer (HGSOC), by combining RIME proteomic analysis, ChIP-seq mapping of genomic binding sites and RNA-seq. Results These studies reveal novel interactions with DNA damage response proteins, in particular, mediator of DNA damage checkpoint protein 1 (MDC1). Through MDC1, ID4 interacts with other DNA repair proteins (γH2AX and BRCA1) at fragile chromatin sites. ID4 does not affect transcription at these sites, instead binding to chromatin following DNA damage. Analysis of clinical samples demonstrates that ID4 is amplified and overexpressed at a higher frequency in BRCA1 -mutant BLBC compared with sporadic BLBC, providing genetic evidence for an interaction between ID4 and DNA damage repair deficiency. Conclusions These data link the interactions of ID4 with MDC1 to DNA damage repair in the aetiology of BLBC and HGSOC.
Identification of slip surfaces in shallow landslides at Aso volcano, Japan based on the fine fraction-plasticity index relationship
Authors: Istiyanti Mega Lia , Goto Satoshi 
Published: March 2020

Abstract
Shallow landslides have frequently occurred in the Aso volcano, Kyushu island, Japan. Yet, observations of the effects of the physical properties of the soil on the landslide stratigraphy have not been explained. In this study, we conducted field observations—at two landslide sites in the Takadake mountain (Aso volcano) area—to identify the slip surfaces. We found that slip surfaces (at both sites) were located in the lower part of the N3-4 Kuroboku soil layer. This was determined by characteristics of the physical properties of the soil, including particle size distribution and plasticity index. Furthermore, we identified the relationship between plasticity index and the fine fraction of the soil to help explain the identification of slip surfaces. Results showed that Kuroboku and Scoria layers have different characteristics according to the plasticity chart (liquid limit-plasticity index relationship) as well as plasticity index-fine fraction relationship.
Periodized resistance training for persistent non-specific low back pain: A mixed methods feasibility study
Authors: Tjøsvoll Svein O. , Mork Paul J. , Iversen Vegard M. , Rise Marit B. , Fimland Marius S. 
Published: March 2020

Abstract
Background We investigated the feasibility of a 16-week supervised heavy resistance training program with weekly undulating periodization for individuals with persistent non-specific low-back pain (LBP). Methods Twenty-five adults with persistent non-specific LBP participated in this mixed methods feasibility study. Participants trained a whole-body program consisting of squat, bench press, deadlift and pendlay row two times per week for 16 weeks. We assessed pain intensity, pain-related disability, pain self-efficacy and one-repetition maximum strength at baseline, 8 weeks and 16 weeks. Three focus group interviews were conducted at the end of the program. Linear mixed models were used to assess changes in outcomes, and the qualitative data was assessed using systematic text condensation. Results We observed clinically meaningful reductions in pain intensity after 8 and 16 weeks of training. The mean difference on the numeric pain rating scale (0-10) in the last two weeks from baseline to 8 weeks was 2.6 (95% CI: 1.8-3.6) and from baseline to 16 weeks 3.4 (95% CI: 2.5-4.4). In addition, there were improvements in pain-related disability (3.9, 95% CI: 2.3-5.5), pain self-efficacy (7.7, 95% CI: 5.4-10.1) and muscle strength. In the focus group interviews, participants talked about challenges regarding technique, the importance of supervision and the advantages of periodizing the training. Perceived benefits were improved pain, daily functioning, energy level and sleep, and changes in views on physical activity. Conclusion Periodized resistance training with weekly undulating periodization is a feasible training method for this group of individuals with persistent non-specific LBP. A randomized clinical trial should assess the efficacy of such an intervention.
An exploration of the protective mechanism of esmolol on the heart of septic rats based on the beta-adrenergic signaling pathway.
Authors: Li Pan , Liu Jingzhuo , Zhang Xintong , Zhen Lingling , Wang Yingzhen , Dong Xiaorong , Zhang Bei , Luo Shuhang , Ma Li 
Published: March 2020

Abstract
Background Cardiac dysfunction caused by sepsis is a serious challenge for ICU doctors. Previous studies showed that the beta adrenergic receptor (β- AR) signaling pathway system can cause abnormal heart function. Studies have also shown that esmolol, a type of beta blocker, participates in the beta receptor desensitization that occurs. This study intends to further explore the specific mechanism of esmolol in the protection of cardiac function.Method In this study, rats were randomly divided into three groups: Sham (sham operation group), CLP (cecal ligations and function group) and ES (esmolol group). The three groups were divided into two subgroups: A and B. Group A was used to observe the number of survival days. Group B was divided into 6 hours,12 hours and 24 hours according to the time after the operation to take samples, and the rats were sacrificed after the corresponding intervention. The inflammatory factors and the factor of β receptor pathway were measured by different methods. And the hypothesis was verified by statistical methods.Results ELISA tests demonstrated that the levels of inflammatory factors CA,TNF-α, and IL-6, as well as G protein pathway factors AC, CAMP and PKA in the CLP group were significantly higher than those in the Sham group, while those factors in the ES group were significantly lower than those in the CLP group, cardiac function factor cTnI is the opposite. Western blotting showed that, compared with the CLP group, the inflammatory proteins MYD88 and NF-KB in the myocardial tissue of the ES group were significantly decreased, while the levels of the beta receptor pathway proteins β1-AR, β2-AR, β-arrestin-1 and β-arrestin-2 were significantly increased, and the result of β-ARK was the opposite. Pathological results and immunofluorescence results indicated that the degree of myocardial cell injury in the ES group was significantly improved compared with that in the CLP group.Conclusion Esmolol has a protective effect on the heart by selectively preventing the pathologic signaling pathway mediated by overactivation of beta receptors while retaining the normal signaling pathway.
Exploring the impact of Artificial Intelligence and Digital Marketing on Intention to Use Online Transportation: a Lesson Learned from Indonesian Millenials
Authors: Pangkey Franycia Maria , Furkan Lalu M. , Herman Lalu Edy , Agusdin Agusdin 
Published: March 2020

Abstract
This paper aims to investigate the influence of artificial intelligence and digital marketing on consumer buying intention. Artificial intelligence development has revolutionized digital marketing and changed consumer buying intention that boosts sales. Artificial intelligence and digital marketing combine the rapidly developing technologies so that everything is reachable in a simple touch that brings best practice to consumers. This paper uses quantitative approach that was stipulated from previous research on artificial intelligence, digital marketing and consumer buying intention. The analysis was carried out using structural equation modeling (SEM) based on partial least square (PLS), with 74 respondents. The novelty of this paper is that it examines the use in online transportation (Gojek) among the millenials. The results showed that artificial intelligence and digital marketing have positively effects on the buying intention. Practical contributions of this research is to inform service organizations and their management in their business development.