bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of the Eutrema Salsugineum EsMYB90 gene important for anthocyanin biosynthesis
Authors: Qi Yuting , Gu Caihong , Wang Xingjun , Gao Shiqing , Li Changsheng , Zhao Chuanzhi , Li Chuanshun , Ma Changle , Zhao Dazhong , Zhang Quan 
Published: December 2019

Abstract
Background: Anthocyanins contribute to coloration and antioxidation effects in different plant tissues. MYB transcription factors have been demonstrated to be a key regulator for anthocyanin synthesis in many plants. However, little information was available about the MYB genes in the halophyte species Eutrema salsugineum.Result: Here we report the identification of an important anthocyanin biosynthesis regulator EsMYB90 from Eutrema salsugineum, which is a halophyte tolerant to multiple abiotic stresses. Our phylogenetic and localization analyses supported that EsMYB90 is an R2R3 type of MYB transcription factor. Ectopic expression of EsMYB90 in tobacco and Arabidopsis enhanced pigmentation and anthocyanin accumulation in various organs. The transcriptome analysis revealed that 42 genes upregulated by EsMYB90 in 35S:EsMYB90 tobacco transgenic plants are required for anthocyanin biosynthesis. Moreover, our qRT-PCR results showed that EsMYB90 promoted expression of early (PAL, CHS, and CHI) and late (DFR, ANS, and UFGT) anthocyanin biosynthesis genes in stems, leaves, and flowers of 35S:EsMYB90 tobacco transgenic plants.Conclusions: Our results indicated that EsMYB90 is a novel MYB transcription factor, which regulates anthocyanin biosynthesis genes to control anthocyanin biosynthesis. Our work provides a new tool to enhance anthocyanin production in various plants.
The DELLA Proteins Interact with MYB21 and MYB24 to Regulate Filament Elongation in Arabidopsis
Authors: Huang Huang , Gong Yilong , Liu Bei , Wu Dewei , Zhang Min , Xie Daoxin , Song Susheng 
Published: December 2019

Abstract
Background: Gibberellin (GA) and jasmonate (JA) are two essential phytohormones for filament elongation in Arabidopsis . GA and JA trigger degradation of DELLAs and JASMONATE ZIM-domain (JAZ) proteins through SCF SLY1 and SCF COI1 separately to activate filament elongation. In JA pathway, JAZs interact with MYB21 and MYB24 to control filament elongation. However, little is known how DELLAs regulate filament elongation. Results: Here we showed that DELLAs interact with MYB21 and MYB24, and that R2R3 domains of MYB21 and MYB24 are responsible for interaction with DELLAs. Furthermore, we demonstrated that DELLA and JAZ proteins coordinately repress the transcriptional function of MYB21 and MYB24 to inhibit filament elongation . Conclusion: We discovered that DELLAs interact with MYB21 and MYB24, and that DELLAs and JAZs attenuate the transcriptional function of MYB21 and MYB24 to control filament elongation. This study reveals a novel cross-talk mechanism of GA and JA in the regulation of filament elongation in Arabidopsis . Keywords: Gibberellin, Jasmonate, MYB21, MYB24, Filament elongation
Clinical-radiological dissociation of a patient with nitrous oxide-induced subacute combined degeneration: A case report
Authors: Jiang Jiwei , Shang Xiuli 
Published: December 2019

Abstract
Background: A number of recent studies have reported subacute combined degeneration (SCD) induced by nitrous oxide (N2O) abuse. However, none have reported the association between the dynamic neuroimaging evolution and clinical manifestations of a patient with N2O-induced SCD.
Case presentation: We describe a 24-year-old man who developed SCD with inverted V-sign hyperintensities over the posterior aspect of the spinal cord caused by frequent, excessive N2O inhalation. One month after treatment, his weakness and paresthesia resolved and his serum vitamin B12 level had improved above normal, but the hyperintensities on T2-weighted images had extended horizontally and longitudinally, compared to the initial magnetic resonance images (MRI). Two months after treatment, the patient had some remaining distal limb numbness and normal serum homocysteine level; however, the abnormal signals seen on cervical T2-weighted images had only slightly decreased compared to those seen on the one-month follow-up MRI. The evolution of conventional MRI findings lagged behind the clinical manifestation, suggesting a clinical-radiological dissociation.
Conclusions: The clinical-radiological dissociation may have occurred in this case because the T2-weighted imaging did not have sufficient sensitivity to reveal cytotoxic edema. Additionally, the serum vitamin B12 level was not a good indicator of cellular vitamin B12. Clinicians should, therefore, recognize this phenomenon, comprehensively assess the condition of patients with N2O-induced SCD, and avoid terminating treatment based on the resolution of clinical symptoms and serological results.
Does the Multiple Sclerosis (MS) Map need to change again? An update of MS prevalence in Mazandaran province of Iran in 2018.
Authors: Baghbanian Seyed Mohammad , Cheraghmakani Hamed , HabibiSaravi Reza , Azar Arash , Ghasemihamedani Fariba 
Published: December 2019

Abstract
Background: Information of Previous studies on the prevalence of MS, including our study conducted twelve years ago, used to shape global prevalence map of MS. According to those results, Iran placed in medium-prevalence MS region in the world Atlas of MS 2013.This study aimed to investigate the prevalence of MS in Mazandaran province after twelve years and the need for possible changes in the global map of the prevalence of MS. Methods: We included all MS patients living in Mazandaran province in 2018 in this descriptive cross-sectional study. We updated our pre-existing registration questionnaires which included demographic information and medical data of MS patients by interview. We obtained the demographic profile of Mazandaran province from the most recent census in 2016 and the National Civil Registry of the Mazandaran province for calculating prevalence of MS. Results: The total number of MS patients in Mazandaran was 2418 (25.8% male and 74.2% female) with a female to male ratio of 2.9. Based on the local population of 3332556, (50.4% male and 49.6% female), this study showed a prevalence of 72.5 per 100,000 for MS in this region. The prevalence of this disease by gender was 37.1 per 100,000 for men and 108.5 per 100,000 for women. The mean (SD) age of the patients at the time of the study was 38.5 (10.1) years with a minimum of 15 and a maximum of 75 years. The most common type of MS was Relapsing-Remitting MS with 86%. Conclusions: All recent studies showed significant upward trend in the prevalence of MS around the world. Based on the results of our study and many other studies in Iran, the Atlas of MS prevalence map needs to be update. Iran's status should be changed to the high-prevalence of MS in the new Atlas. Due to the increasing prevalence of MS, we suggest an adjustment in the Global MS Prevalence Scale.
Представление о психологическом иммунитете в отечественной психологии
Authors: Москаленко Галина В. , Зелинская Ирина А. 
Published: December 2019

Introduction. Numerous multidisciplinary studies examine non-physical types of immunity in recent years. By analogy with physical immunity, psychological immunity provides the comprehensive protection of the human psyche in extreme stressful situations and also in everyday activities. Psychological immunity can potentially become the phenomenon that may combine disparate views of defense mechanisms of the human psyche. Therefore, it seems reasonable to introduce and develop this concept.
Theoretical Basis. The concept of psychological immunity was first introduced by A. Olah. He defined it as a mental phenomenon that brings together all the adaptive resources of an individual. Previous research has demonstrated that psychological immunity is a widely accepted concept with clear conceptual and theoretical grounds and practical tools for diagnosis, development, and correction. The development of the concept of psychological immunity aroused our interest in Russian researches in this field.
Results. Russian researchers agree that psychological immunity is a personality trait that contributes to the preservation of an individual’s adaptive state when exposed to adverse factors through the use of resources. These resources are not only psychological defenses and coping strategies, but also self-regulation, reflection, consciousness, meaningfulness, coherence, etc. Very few but diverse previous studies of psychological immunity have outlined its content-related, structural, and functional concept.
Discussion. Psychological immunity is a mental phenomenon that helps to maintain the state of psychological well-being and psychological safety. The functions of psychological immunity are identical to those of physical immunity and include monitoring the functional state, memorizing the impact, reducing the intensity of re-experiencing, maintaining the state of psychological well-being, and facilitating negative experience. Aversion (as a reaction to potentially unpleasant phenomena) and anxiety (as a reaction to potentially dangerous phenomena) are the mechanisms that trigger a psychoimmune response.
Molecular and Genomic Characterization of PFAB2: A Non-virulent Bacillus anthracis Strain Isolated from an Indian Hot Spring
Authors: Banerjee Aparna , Somani Vikas K. , Chakraborty Priyanka , Bhatnagar Rakesh , Varshney Rajeev K. , Echeverría-Vega Alex , Cuadros-Orellana Sara , Bandopadhyay Rajib 
Published: December 2019

Background:
Thermophilic bacilli in both aerobic or facultative anaerobic forms have been
isolated for over a hundred years from different mesophilic or thermophilic environments as they are
potential source of bioactive secondary metabolites. But the taxonomic resolution in the Bacillus genus
at species or at strain level is very challenging for the insufficient divergence of the 16S rRNA
genes. One such recurring problem is among Bacillus anthracis, B. cereus and B. thuringiensis. The
disease-causing B. anthracis strains have their characteristic virulence factors coded in two wellknown
plasmids, namely pXO1 (toxin genes) and pXO2 (capsule genes).

Objective:
The present study aimed at the molecular and genomic characterization of a recently reported
thermophilic and environmental isolate of B. anthracis, strain PFAB2.

Methods:
We performed comparative genomics between the PFAB2 genome and different strains of
B. anthracis, along with closely related B. cereus strains.

Results:
The pangenomic analysis suggests that the PFAB2 genome harbors no complete prophage
genes. Cluster analysis of Bray-Kurtis similarity resemblance matrix revealed that gene content of
PFAB2 is more closely related to other environmental strains of B. anthracis. The secretome analysis
and the in vitro and in vivo pathogenesis experiments corroborate the avirulent phenotype of this
strain. The most probable explanation for this phenotype is the apparent absence of plasmids harboring
genes for capsule biosynthesis and toxins secretion in the draft genome. Additional features of
PFAB2 are good spore-forming and germinating capabilities and rapid replication ability.

Conclusion:
The high replication rate in a wide range of temperatures and culture media, the nonpathogenicity,
the good spore forming capability and its genomic similarity to the Ames strain together
make PFAB2 an interesting model strain for the study of the pathogenic evolution of B. anthracis.
Exploring PLAC1 Structure and Underlying Mechanisms to Design a Derivative Vaccine Against Breast Cancer Progression; In-Silico Study
Authors: Afzali Farzaneh , Ghahremanifard Parisa , Ranjbar Mohammad Mehdi , Salimi Mahdieh 
Published: December 2019

Background:
The tolerogenic homeostasis in Breast Cancer (BC) can be surpassed by rationally
designed immune-encouraging constructs against tumor-specific antigens through immunoinformatics
approach.

Objective:
Availability of high throughput data providing the underlying concept of diseases and awarded
computational simulations, lead to screening the potential medications and strategies in less time and cost.
Despite the extensive effects of Placenta Specific 1 (PLAC1) in BC progression, immune tolerance,
invasion, cell cycle regulation, and being a tumor-specific antigen the fundamental mechanisms and
regulatory factors were not fully explored. It is also worth to design an immune response inducing construct
to surpass the hurdles of traditional anti-cancer treatments.

Methods and Result:
The study was initiated by predicting and modelling the PLAC1 secondary and
tertiary structures and then engineering the fusion pattern of PLAC1 derived immunodominant predicted
CD8+ and B-cell epitopes to form a multi-epitope immunogenic construct. The construct was analyzed
considering the physiochemical characterization, safety, antigenicity, post-translational modification,
solubility, and intrinsically disordered regions. After modelling its tertiary structure, proteinprotein
docking simulation was carried out to ensure the attachment of construct with Toll-Like Receptor
4 (TLR4) as an immune receptor. To guarantee the highest expression of the designed construct in
E. coli k12 as an expressional host, the codon optimization and in-silico cloning were performed. The
PLAC1 related miRNAs in BC were excavated and validated through TCGA BC miRNA-sequencing
and databases; the common pathways then were introduced as other probable mechanisms of PLAC1
activity.

Conclusion:
Regarding the obtained in-silico results, the designed anti-PLAC1 multi-epitope construct
can probably trigger humoral and cellular immune responses and inflammatory cascades, therefore
may have the potential of halting BC progression and invasion engaging predicted pathways.
Pengembangan multimedia pembelajaran interaktif berbasis video materi sikap cinta tanah air dan peduli lingkungan
Authors: Nugroho Irfan Adi , Surjono Herman Dwi 
Published: December 2019

Penelitian ini memiliki tujuan: (1) menghasilkan multimedia pembelajaran interaktif berbasis video yang layak meningkatkan sikap cinta tanah air dan peduli lingkungan Gugus Gajah Mada, Dawe, Kudus, (2) mengetahui kefektifan multimedia pembelajaran interaktif berbasis video dalam meningkatkan sikap cinta tanah air dan peduli lingkungan di SD kelas V gugus gajah Dawe Kudus dikembangkan. Multimedia yang telah disusun di uji oleh ahli, menggunakan angket dan dihitung menggunakan skala lima, untuk hasil dari sikap cinta tanah air juga menggunakan angket sebagai sara untuk menggali data. Hasil analisis data menunjukkan multimedia yang disusun layak digunakan sebagai media pembelajaran, terbukti dari empat aspek penilaian memperoleh nilai yang berkategori minimal baik. Tampilan 3,93, program 4, 08 pembelajaran 3,71 dan ini 4,20. Uji efektifitas menunjukkan data bahwa media yang telah dikembangkan efektif untuk meninkatkan siap cinta tanah air dan peduli lingkungan, terbukti dari perbandingan cinta tanah air yang tidak menggunakan media memperoleh rata-rata 335,96 dan yang menggunakan media 1107,93 bagi siswa yang menggunakan media. Sikap peduli lingkungan yang tidak menggunakan media memperoleh rata-rata 298,52 dan yang menggunakan media 944,20.Kata kunci: Multimedia interaktif, cinta tanah air, peduli lingkunganDeveloping video-based interactive teaching multimedia in terms of the nationalism and environmental awarenessAbstractThe purposes of the research are: (1) creating reliable-interactive based video learning multimedia to improve nationalism and environmental awareness of Gajah Mada, Dawe, Kudus Division, (2) finding out its effectiveness to improve nationalism and the awareness. The arrange multimedia is tested by the experts using questionnaire and counted through the use of five scale. In other hand, for nationalism, the use of questionnaire is used to collect data. The data analysis shows the arranged multimedia is reliable to use as learning media, proven from four assessed aspects gets fair minimum category. The scores are appearance 3.93, program 4.08, learning 3.71, the core is 4.20. Its test of effectiveness shows the developed media is effective to improve the nationalism and the awareness, proven from its comparison compared to those averages of not using the media 335.96, while the one utilizing it 1107.93. The awareness of those not utilizing it gains average 298.52 while the utilizing one 944,20. Keywords: Interactive multimedia, nationalism, environmental-awereness
Pengembangan game kuis interaktif sebagai instrumen evaluasi formatif pada mata kuliah teori musik
Authors: Ardiningsih Dini 
Published: December 2019

Penelitian ini bertujuan untuk menghasilkan sebuah game kuis interaktif sebagai instrumen evaluasi formatif pada mata kuliah teori musik dengan mengadaptasi sebuah game kuis The Impossible Quiz. Game kuis berisi pertanyaan dengan materi teori musik yang dilengkapi dengan ear training. Metode penelitian yang digunakan adalah metode Research and Development model ADDIE yaitu Analysis (analisis), Design (desain), Development (pengembangan), Implementation (implementasi) dan Evaluation (evaluasi). Penelitian dilakukan di Sekolah Tinggi Musik Bandung (STiMB). Kelayakan produk berdasarkan penilaian ahli materi, ahli media dan responden mahasiswa STiMB. Penilaian berupa lembar evaluasi yang meliputi empat aspek yaitu aspek materi, aspek pembelajaran, aspek tampilan dan aspek pemrograman. Secara keseluruhan rerata presentasi penilaian yaitu (1) aspek pembelajaran sebesar 79,67%, (2) aspek materi sebesar 87,81%, (3) aspek pemrograman sebesar 81,87%, dan (4) aspek tampilan game sebesar 85,15%. Dengan demikian dapat disimpulkan bahwa rerata penilaian di atas 80% yang berarti game kuis interaktif sangat layak dijadikan instrumen evaluasi formatif pada mata kuliah teori musik.Kata kunci: game kuis, the impossible quiz, interaktif, teori musik, ADDIEDeveloping interactive quiz game as formtive evaluation instrument of music theory subjectAbstractThis research was aim to produce an interactive game quiz as a instrument for formative evaluation of subject Music Theory adapted from The Impossible Quiz. The game consist of Music Theory learning materials and be equipped with ear training materials. This research was conducted by Research & Development method with ADDIE model which is Analysis, Design, Development, Implementation and Evaluation. All of research process were held at Sekolah Tinggi Musik Bandung (STiMB). Properness of the reasearch was evaluated by Music Theory expert, multimedia expert and students of Sekolah Tinggi Musik Bandung. Four aspects were have been evaluated by the respondents includes interface aspect, contents, learning materials and programming aspect. In average, learning materials got precentage of 79,67%, contents 87,81%, programming 81,87%, and interface aspect 85,15%. Those result conduct conclusion of 80% rating of this evaluation which means the game quiz are appropriate to become a instrument for formative evaluation on Music Theory subject.Keywords: quiz game, the impossible quiz, interactive, music theory, ADDIE
Evaluasi pelaksanaan co-teaching blended learning bahasa Inggris bidang kewarganegaraan
Authors: The Hery Yanto , Awaliyah Siti 
Published: December 2019

Penelitian ini bertujuan memaparkan perancangan, pelaksanaan, dan evaluasi perkuliahan Bahasa Inggris Kewarganegaraan di Universitas Malang. Selain itu, penelitian ini juga menyajikan contoh lingkungan pembelajaran blended learning dengan menggunakan multimedia yang efektif untuk meningkatkan motivasi belajar mahasiswa di bidang Bahasa Inggris Kewarganegaraan. Pendekatan mixed-method digunakan untuk mengumpulkan, menyajikan, dan menganalisis data. Portofolio kegiatan instruksional dan kuisioner adalah bentuk data yang digunakan. Data disajikan dan dianalisis menggunakan narasi deskriptif, studi kasus, analisis dokumen, dan analisis statistik deskriptif. Hasil penelitian menunjukkan kolaborasi dalam bentuk co-teaching blended learning dapat meningkatkan kualitas pembelajaran. Kedua instruktur yang terlibat dalam kolaborasi dapat saling mendukung untuk mencapai tujuan pembelajaran. Selain diperlukan keahlian di dalam mengajar, instruktur juga perlu menguasai cara perancangan instruksional. Mahasiswa secara aktif bekerja secara berpasangan, berdiskusi, dan berkelompok di dalam menyelesaikan tugas pembelajaran di dalam kelas dan di Edmodo. Interaksi mahasiswa dengan materi belajar dan instruktur juga dapat difasilitasi dengan manajemen sistem belajar dan teknologi pembelajaran daring seperti VoiceThread, Skype, VSee, dan posel.Kata kunci: blended learning, pembelajaran multimedia Evaluating the implementation of co-teaching blended learning the English for specific purposes in civic education programAbstractThis study reveals the design, implementation, and evaluation of the English for Specific Purposes Course in Civic Education Program at the State University of Malang. The course multimedia learning environment was designed based on the blended learning principals. This learning environment is effective to increase students’ motivation. The study applied mixed-method approaches to collect, present, and analyze data. The instructional portfolio and a questionnaire were used as data. The narrative descriptive, case study, document analysis, and descriptive statistics principals were applied to analyze and present data. The results showed that co-teaching in the form of blended learning can improve the quality of the course and students’ learning. Instructors can support one another collaboratively to achieve the learning objectives. Besides teaching skills, the instructors should acquire sufficient instructional design skills. Students were identified actively engaging in pair works, discussions, and groups’ works to complete their learning tasks in class and in Edmodo. Interactions are mediated sufficiently by the learning management system and learning technologies, such as VoiceThread, Skype, VSee, and email. Keywords: Blended learning, multimedia learning