bluuebunny/crossref_metadata_2025_split huggingface dataset:
Profiling of hydroxyurea‐treated β‐thalassemia/ serum proteome through nano‐LC–ESI–MS/ MS in combination with microsol‐isoelectric focusing
Authors: Khan Faisal , Ali Arslan , Iqbal Ayesha , Musharraf Syed Ghulam 
Published: November 2019

AbstractAdvancements in proteomic tools offer a comprehensive solution to studying the complexity of diseases at molecular level. This study focusses on the clinical proteomic profiling of pre‐ and post‐hydroxyurea (HU)‐treated β‐thalassemia patients in parallel with healthy individuals to better understand the role of HU in the treatment of β‐thalassemia. The strategy encompasses sequential high‐resolution protein fractionation using MicroSol‐isoelectric focusing (ZOOM‐ IEF) followed by one‐dimensional SDS‐PAGE before nano‐RP‐LC–MS/ MS analysis of tryptic peptides. Protein identification was performed through Mascot search using NCBInr and SwissProt databases. Several different proteins were observed in pool serum samples of each of the three study groups. Approximately, 1250 proteins exclusive to each group were identified, and after removing the redundant and low sequence coverage proteins, the number was reduced to 576 (201 in healthy, 187 in HU‐untreated and 188 in HU‐treated group). Uniquely identified proteins in the HU‐treated group regulate the focal adhesion, ECM‐receptor interaction, PI3K‐Akt signaling, Rap1 signaling, cAMP signaling, platelet activation, and Ca2+ signaling pathways in the HU‐treated group. The proteomic profile presented here will add to the current state of understanding of molecular mechanisms involved in hydroxyurea treatment of β‐thalassemia.
Rapid quantitative analysis of 18 chemical constituents in HuangQi JianZhong Tang based on UHPLC–MS
Authors: Liu Yuetao , Jia Lu , Qin Xuemei 
Published: November 2019

AbstractA rapid, highly sensitive, and specific analytical method using ultra‐high‐performance liquid chromatography‐triple quadruple MS was developed to quantitatively measure 18 chemical constituents in Huangqi Jianzhong Tang (HQJZ), a famous traditional Chinese medicine formula. Chromatographic separation was performed on a Waters ACQUITY UPLC HSS T3 column (2.1 mm × 100 mm, 1.8 μm) with a gradient mobile phase (A: 0.1% aqueous formic acid and B: acetonitrile) at a flow rate of 0.25 mL/min. The chromatographic peaks of 18 components were identified by comparing with the reference compounds. Multiple reaction monitoring was employed for quantitative analysis of the components. Seven batches of HQJZ samples were analyzed with a good linear regression relationship (R2, .9978–.9993), precisions [relative standard deviation (RSD), 0.90%–3.60%], repeatability (RSD, 2.50%–4.00%), stability (RSD, 1.00%–4.00%), and recovery (96.10%–104.30%). Based on this established method, the present study offered a highly sensitive, specific, and rapid determination method for identification of 18 compounds, which greatly promoted the systemic quality control of HQJZ.
GC–MS analysis of organic acids in rat urine: A protocol of direct ultrasound‐assisted derivatization
Authors: Yu Jiaying , Liu Xiaowei , Guo Jing , Zhao Jinhui , Li Ying , Sun Changhao , Liu Liyan 
Published: November 2019

AbstractThe aim of the present study was to develop a novel ultrasound‐assisted derivatization method for analysis of urine that can be used for preliminary screening and monitoring of metabolic disorders. Here we describe an ultrasound‐assisted derivatization method followed by GC–MS analysis to quantify 26 organic acids in urine. The optimum levels of the variables affecting the yield of derivatization were investigated, including urease doses, derivatization reagents and derivatization conditions (duration time, reaction temperature and sonic power). The method exhibited the best results with 80 μl urease. The optimal reaction conditions were 100 μl BSTFA, 80% ultrasound power, 70°C and 40 min. This method showed satisfactory linearity, good reproducibility and an acceptable limit of detection and accuracy. Therefore, it could potentially be used to as a standard method to enable comparisons between laboratories. Finally, we applied our method to urine samples from pregnant rats administered 2 or 10 mg/kg folic acid supplementation.
Cardoon as a Sustainable Crop for Biomass and Bioactive Compounds Production
Authors: Barracosa Paulo , Barracosa Mariana , Pires Euclides 
Published: November 2019

AbstractCardoon is a multi‐purpose and versatile Mediterranean crop, adapted to climate change, with a wide spectrum of potential applications due its added value as a rich source of fibers, oils and bioactive compounds. The Cynara species are a component of the Mediterranean diet and have been used as food and medicine since ancient times. The important role of cardoon in human nutrition, as a functional food, is due to its high content of nutraceutical and bioactive compounds such as oligofructose inulin, caffeoylquinic acids, flavonoids, anthocyanins, sesquiterpenes lactones, triterpenes, fatty acids and aspartic proteases. The present review highlights the characteristics and functions of cardoon biomass which permits the development of innovative products in food and nutrition, pharmaceutics and cosmetics, plant protection and biocides, oils and energy, lignocellulose materials, and healthcare industries following the actual trends of a circular economy.
Assimilation in Suburbia? Geographical and Cultural Barriers to Working With LGBTQ+ Students in Suburban Community Colleges
Authors: Edwards Monica , Grippe Al 
Published: November 2019

AbstractPartial LGBTQ+ assimilation into mainstream culture has changed how practitioners interact with students at suburban community colleges. This chapter reviews the history of the Chicago suburbs and reflects on the authors' experiences working with LGBTQ+ students in community colleges there.
Outcomes with retrograde versus antegrade chronic total occlusion revascularization
Authors: Megaly Michael , Ali Abdelrahman , Saad Marwan , Omer Mohamed , Xenogiannis Iosif , Werner Gerald S. , Karmpaliotis Dimitri , Russo Juan J. , Yamane Masahisa , Garbo Roberto , Gagnor Andrea , Ungi Imre , Rinfret Stephane , Pershad Ashish , Wojcik Jaroslaw , Garcia Santiago , Mashayekhi Kambis , Sianos Georgios , Galassi Alfredo R. , Burke M. Nicholas , Brilakis Emmanouil S. 
Published: November 2019

AbstractObjectivesThe aim of the study was to evaluate the outcomes of retrograde versus antegrade approach in chronic total occlusion (CTO) percutaneous coronary intervention (PCI).BackgroundThe retrograde approach has increased the success rate of CTO PCI but has been associated with a higher risk for complications.MethodsWe conducted a meta‐analysis of studies published between 2000 and August 2019 comparing the in‐hospital and long‐term outcomes with retrograde versus antegrade CTO PCI.ResultsTwelve observational studies (10,240 patients) met our inclusion criteria (retrograde approach 2,789 patients, antegrade approach 7,451 patients). Lesions treated with the retrograde approach had higher J‐CTO score (2.8 vs. 1.9, p < .001). Retrograde CTO PCI was associated with a lower success rate (80.9% vs. 87.4%, p < .001). Both approaches had similar in‐hospital mortality, urgent revascularization, and cerebrovascular events. Retrograde CTO PCI was associated with higher risk of in‐hospital myocardial infarction (MI; odds ratio [OR] 2.37, 95% confidence intervals [CI] 1.7, 3.32, p < .001), urgent pericardiocentesis (OR 2.53, 95% CI 1.41–4.51, p = .002), and contrast‐induced nephropathy (OR 2.12, 95% CI 1.47–3.08; p < .001). During a mean follow‐up of 48 ± 31 months retrograde crossing had similar mortality (OR 1.79, 95% CI 0.84–3.81, p = .13), but a higher incidence of MI (OR 2.07, 95% CI 1.1–3.88, p = .02), target vessel revascularization (OR 1.92, 95% CI 1.49–2.46, p < .001), and target lesion revascularization (OR 2.08, 95% CI 1.33–3.28, p = .001).ConclusionsCompared with antegrade CTO PCI, retrograde CTO PCI is performed in more complex lesions and is associated with a higher risk for acute and long‐term adverse events.
Successful treatment of a case with NUT midline carcinoma in the larynx and review of the literature
Authors: Zhang Hai , Liu Ming‐Hua , Zhang Jie , Luo Shu‐Peng , Wang Chao‐Bo , Zhao Zeng‐Hu , Ge Yan‐Li , Zhang Jian‐Yu , Geng Feng‐Hao 
Published: November 2019

AbstractIn this report, we gave the first case of successful treatment for laryngeal NMC, which is exceedingly rare with dismal prognosis. intensity‐modulated radiation therapy accompanied by traditional Chinese medicine was administrated for the young woman, instead of radical resection, and she got continuous remission for more than 2 years, with no recurrence detected.
Aqueous Phase Aldol Condensation of Formaldehyde and Acetone on Anatase TiO2(101) Surface: A Theoretical Investigation
Authors: Zhao Yuntao , Zhu Xinli , Wang Hua , Han Jinyu , Mei Donghai , Ge Qingfeng 
Published: November 2019

AbstractA mechanistic understanding of catalytic reactions at solid‐liquid interface is limited both experimentally and theoretically but attracts much interest. Using density functional theory calculations (DFT) and ab initio molecular dynamics (AIMD) simulations, we investigated the effect of liquid water on α‐H abstraction, C−C coupling, and dehydration steps of aldol condensation of formaldehyde and acetone on an anatase TiO2(101) surface. The existence of the aqueous phase lowered the Gibbs energy of activation of dehydration step pronouncedly from 187 to 74 kJ/mol through proton transfer mechanism, making the hydrogenation pathway more favorable in the aqueous phase. In contrast, a mixed route prevails in the vapor phase. This work provides insights into the effect of the bulk water through a proton transfer mechanism on the dehydrogenation, C−C coupling, and dehydration steps.
Vergleich Hoch‐Wärmeintegrierter Adsorberkonzepte für eine Anwendung in Direct‐Air‐Capture‐Verfahren
Authors: Drechsler Carsten , Agar David W. 
Published: November 2019

AbstractZur Überwindung des hohen kohlenstoffdioxidspezifischen Energieeintrags bei Temperaturwechseladsorptionskonzepten für Direct‐Air‐Capture‐Prozesse wird eine Anwendung hoch‐wärmeintegrierter Verfahrenskonzepte untersucht. Die detaillierte Modellierung und Simulation eines Band‐ als auch Wanderbettadsorberkonzeptes deutet darauf hin, dass eine Rückgewinnung der sensiblen Wärme des Adsorbens von mehr als 90 % erreicht werden kann. Hier eröffnet eine Kombination der vorgestellten Konzepte mit einem Power‐to‐Gas‐Verfahren das Potenzial zur Realisierung eines autothermen Gesamtprozesses.
Power‐to‐X: Between Electricity Storage, e‐Production, and Demand Side Management
Authors: Burre Jannik , Bongartz Dominik , Brée Luisa , Roh Kosan , Mitsos Alexander 
Published: November 2019

AbstractThe common understanding of Power‐to‐X is exclusively the use of renewable electricity to manufacture products currently based on fossil sources. In this paper, it is argued that beyond such e‐Production many of these technologies also include aspects related to demand side management and temporal storage of electricity. Therefore, a definition of Power‐to‐X is suggested that encompasses all three aspects. It is discussed, which of these are relevant under which conditions and illustrative examples are highlighted, which show how process systems engineering can help address common challenges for Power‐to‐X technologies.
Monocyclic Quinone Structure‐Activity Patterns: Synthesis of Catalytic Inhibitors of Topoisomerase II with Potent Antiproliferative Activity
Authors: Waugh Thomas M. , Masters John , Aliev Abil E. , Marson Charles M. 
Published: November 2019

AbstractThe monocyclic 1,4‐benzoquinone, HU‐331, the direct oxidation product of cannabidiol, inhibits the catalytic activity of topoisomerase II but without inducing DNA strand breaks or generating free radicals, and unlike many fused‐ring quinones exhibits minimal cardiotoxicity. Thus, monocyclic quinones have potential as anticancer agents, and investigation of the structural origins of their biological activity is warranted. New syntheses of cannabidiol and (±)‐HU‐331 are here reported. Integrated synthetic protocols afforded a wide range of polysubstituted resorcinol derivatives; many of the corresponding novel 2‐hydroxy‐1,4‐benzoquinone derivatives are potent inhibitors of the catalytic activity of topoisomerase II, some more so than HU‐331, whose monoterpene unit replaced by a 3‐cycloalkyl unit conferred increased antiproliferative properties in cell lines with IC50 values extending below 1 mM, and greater stability in solution than HU‐331. The principal pharmacophore of quinones related to HU‐331 was identified. Selected monocyclic quinones show potential for the development of new anticancer agents.
A Thorough QT Study to Evaluate the Effects of a Supratherapeutic Dose of Sertraline on Cardiac Repolarization in Healthy Subjects
Authors: Abbas Richat , Riley Steve , LaBadie Robert R. , Bachinsky Mary , Chappell Phillip B. , Crownover Penelope H. , Damle Bharat 
Published: November 2019

AbstractThe effect of steady‐state supratherapeutic sertraline (Zoloft) on QT interval was assessed in a single‐center, randomized, 3‐way crossover, double‐blind, placebo‐ and moxifloxacin‐controlled thorough QT study. Healthy adults received sertraline 400 mg/day, moxifloxacin 400 mg, and placebo, with a washout period (≥14 days) between treatments. A 12‐lead electrocardiogram was recorded in triplicate before dosing and at selected time points up to 72 hours after dosing. Analysis of covariance using a mixed‐effect model with sequence, period, treatment, time, and treatment‐by‐time interaction as fixed effects; subject within sequence as a random effect; and baseline QT corrected for heart rate using Fridericia formula (QTcF) as a covariate was conducted. A 90% confidence interval for the least squares (LS) mean difference in QTcF between active treatment and placebo was computed for each postdose time point. Exposure‐response was assessed using linear mixed‐effect modeling. Fifty‐four subjects were enrolled. Over 24 hours after dosing, the LS mean difference in QTcF for sertraline versus placebo ranged from 5.597 milliseconds to 9.651 milliseconds. The upper bound of the 90% confidence interval for the LS mean difference exceeded a predefined 10‐millisecond significance threshold at the 4‐hour postdose time point only (LS mean, 9.651 milliseconds [90% confidence interval, 7.635‐11.666]). In the exposure‐response analysis, QTcF values increased significantly with increasing sertraline concentration (slope = 0.036 milliseconds/ng/mL; P < .0001). Predicted change from baseline in QTcF at therapeutic maximum plasma sertraline concentration was 3.57 milliseconds. This thorough QTc study demonstrated a positive signal for QTc prolongation for sertraline at the steady‐state 400‐mg/day dose.
Benchmarking food and beverage company investment in healthful eating and active living initiatives
Authors: Barata Cavalcanti Olivia , Costa Sergio A. , Ferris Emily , Guillermin Michelle , Palmedo Chris , Crossley Rachel , Huang Terry T.‐K. 
Published: November 2019

AbstractWe benchmarked the strategies and performance of community‐based healthful eating and active living initiatives sponsored by food and beverage manufacturers. We developed company‐ and program‐level surveys for 11 companies and 38 programs using best practices in corporate benchmarking and the collective impact framework, including four domains: (a) strategy design, (b) governance and management, (c) monitoring and evaluation, and (d) reporting, communication, and stakeholder engagement. Company strategies varied, with most focusing on nutrition and physical activity promotion and little on social and environmental determinants of health. Some companies reported program investments as part of corporate social responsibility, and others managed these under general corporate or public affairs. Company scores ranged broadly, suggesting that our metrics had discriminant validity (mean overall score=59%, range=49–72%). Companies performed worst on monitoring and evaluation, highlighting the need for metrics and accountability. Benchmarking ensures accountability and enhances impact of corporate social responsibility initiatives.
P‐type non‐isolated boost DC‐DC converter with high voltage gain and extensibility for DC microgrid applications
Authors: Mohammadi Farid , Farhadi‐Kangarlu Mohammad , Rastegar Hassan , Khorsandi Amir , Pichan Mohammad 
Published: November 2019

SummaryIn this paper, the concept of converter design, using the least number of elements and achieving high voltage gain at the low duty cycle, is proposed for the microgrids. One of the important issues in the microgrids is boosting the low voltage output of sources to the utility voltage level. Therefore, the step‐up DC‐DC converters are widely used in these systems to attain the utility voltage. The benchmarking of the converters mainly in terms of the voltage gain, efficiency, the number of active and passive components, stresses on semiconductors, and simplicity is considered. In this paper, a new extendable non‐isolated boost DC‐DC converter is presented. Comparing the conventional boost converter, the basic structure of the proposed converter has a high voltage gain and reduced stress on the switch. To increase the voltage gain, the basic structure of the proposed converter can be easily extended. The modulation technique employed is high‐frequency pulse‐width modulation (PWM). The detailed analysis of the proposed converter in continuous current mode (CCM) and discontinuous current mode (DCM) is presented. The relations between currents and voltages and the voltage gain in CCM and DCM are obtained. Experimental results are carried out to verify theoretical concepts by using the hardware prototype.
Molecular mechanisms of homeostatic plasticity in central pattern generator networks
Authors: Northcutt Adam J. , Schulz David J. 
Published: November 2019

AbstractCentral pattern generator (CPG) networks rely on a balance of intrinsic and network properties to produce reliable, repeatable activity patterns. This balance is maintained by homeostatic plasticity where alterations in neuronal properties dynamically maintain appropriate neural output in the face of changing environmental conditions and perturbations. However, it remains unclear just how these neurons and networks can both monitor their ongoing activity and use this information to elicit homeostatic physiological responses to ensure robustness of output over time. Evidence exists that CPG networks use a mixed strategy of activity‐dependent, activity‐independent, modulator‐dependent, and synaptically regulated homeostatic plasticity to achieve this critical stability. In this review, we focus on some of the current understanding of the molecular pathways and mechanisms responsible for this homeostatic plasticity in the context of central pattern generator function, with a special emphasis on some of the smaller invertebrate networks that have allowed for extensive cellular‐level analyses that have brought recent insights to these questions.
Evaluating the effects of large marine predators on mobile prey behavior across subtropical reef ecosystems
Authors: Phenix Lindsay M. , Tricarico Dana , Quintero Enrique , Bond Mark E. , Brandl Simon J. , Gallagher Austin J. 
Published: November 2019

AbstractThe indirect effect of predators on prey behavior, recruitment, and spatial relationships continues to attract considerable attention. However, top predators like sharks or large, mobile teleosts, which can have substantial top–down effects in ecosystems, are often difficult to study due to their large size and mobility. This has created a knowledge gap in understanding how they affect their prey through nonconsumptive effects. Here, we investigated how different functional groups of predators affected potential prey fish populations across various habitats within Biscayne Bay, FL. Using baited remote underwater videos (BRUVs), we quantified predator abundance and activity as a rough proxy for predation risk and analyzed key prey behaviors across coral reef, sea fan, seagrass, and sandy habitats. Both predator abundance and prey arrival times to the bait were strongly influenced by habitat type, with open homogenous habitats receiving faster arrival times by prey. Other prey behaviors, such as residency and risk‐associated behaviors, were potentially driven by predator interaction. Our data suggest that small predators across functional groups do not have large controlling effects on prey behavior or stress responses over short temporal scales; however, habitats where predators are more unpredictable in their occurrence (i.e., open areas) may trigger risk‐associated behaviors such as avoidance and vigilance. Our data shed new light on the importance of habitat and context for understanding how marine predators may influence prey behaviors in marine ecosystems.
Differential expression of olfactory genes in Atlantic salmon (Salmo salar) during the parr–smolt transformation
Authors: Madsen Steffen S. , Winther Sara S. T. , Bollinger Rebecca J. , Steiner Ulrich , Larsen Martin H. 
Published: November 2019

AbstractThe anadromous salmon life cycle includes two migratory events, downstream smolt migration and adult homing migration, during which they must navigate with high precision. During homing migration, olfactory cues are used for navigation in coastal and freshwater areas, and studies have suggested that the parr–smolt transformation has a sensitive period for imprinting. Accordingly, we hypothesized that there would be significant changes in gene expression in the olfactory epithelium specifically related to smoltification and sampled olfactory rosettes from hatchery‐reared upper growth modal juvenile Atlantic salmon at 3‐week intervals from January to June, using lower growth modal nonsmolting siblings as controls. A suite of olfactory receptors and receptor‐specific proteins involved in functional aspects of olfaction and peripheral odor memorization was analyzed by qPCR. Gene expression in juveniles was compared with mature adult salmon of the same genetic strain caught in the river Gudenaa. All mRNAs displayed significant variation over time in both modal groups. Furthermore, five receptor genes (olfc13.1, olfc15.1, sorb, ora2, and asor1) and four olfactory‐specific genes (soig, ependymin, gst, and omp2) were differentially regulated between modal groups, suggesting altered olfactory function during smoltification. Several genes were differentially regulated in mature salmon compared with juveniles, suggesting that homing and odor recollection involve a different set of genes than during imprinting. Thyroid hormone receptors thrα and thrβ mRNAs were elevated during smolting, suggesting increased sensitivity to thyroid hormones. Treatment of presmolts with triiodothyronine in vivo and ex vivo had, however, only subtle effects on the investigated olfactory targets, questioning the hypothesis that thyroid hormones directly regulate gene expression in the olfactory epithelium.