bluuebunny/crossref_metadata_2025_split huggingface dataset:
Supernumerary maxillary fourth premolar teeth in five related Burmese cats
Authors: Emslie Roxanne S , Tutt Cedric LC 
Published: October 2020

Case series summary This article reports a case series of five Burmese cats with supernumerary teeth. All included cases displayed either unilateral or bilateral supernumerary maxillary fourth premolar teeth. Two cases also displayed supernumerary mandibular fourth premolar teeth, and in one case bilateral supernumerary maxillary third incisor teeth were found. Examination of the pedigrees of the affected cats revealed that all five cats were related with one common ancestor. All patients in this case series were successfully treated. Relevance and novel information Supernumerary maxillary fourth premolar teeth in cats have not been reported previously in the literature. As all five cats in this case series were shown to be related, supernumerary maxillary fourth premolar teeth may be yet another heritable health condition affecting the Burmese breed. Therefore, Burmese breeders should endeavour to reduce the incidence of supernumerary teeth within the breed by having animals examined by a veterinary dentist prior to breeding, and neutering affected, as well as related, individuals. Burmese cat owners must be made aware of the condition in order to institute early evaluation, diagnosis and treatment.
The Experiences and Perceptions of Health-Care Workers During the COVID-19 Pandemic in Muscat, Oman: A Qualitative Study
Authors: Al Ghafri Thamra , Al Ajmi Fatma , Anwar Huda , Al Balushi Lamya , Al Balushi Zainab , Al Fahdi Fatma , Al Lawati Anwaar , Al Hashmi Salwa , Al Ghamari Aisha , Al Harthi Mohamed , Kurup PadmaMohan , Al Lamki Mustafa , Al Manji Asim , Al Sharji Abdallah , Al Harthi Saud , Gibson Emma 
Published: October 2020

Introduction Predominantly, studies on COVID-19 report quantitative data that often miss the social implications and other determinants of health. The objective of this study was to explore the experiences and perceptions of health care workers (HCWs) in primary health care in the management of COVID-19 with respect to medical response experiences, socio-cultural and religious reforms, psychological impressions, and lessons learned. Methods This was a qualitative study using an empirical phenomenological approach. Six focus group discussions were conducted across various stakeholders working frontline in the management of COVID-19 (managerial, public health/field/community and primary care health centers). They participated in semi-structured, in-depth group discussions from 11th to 20th May 2020. All discussions were audio-recorded, transcribed verbatim and analyzed using thematic analysis. Results Forty participants were involved in this study. Three themes emerged related to the medical response experiences, including the rapid re-structuring of the PHC services, use of technology and challenges of working on COVID-19. Perceptions on the socio-cultural and religious reforms included changes in social and religious norms, and anticipated gaps in accessing health care among the vulnerable groups (elderly, expatriates, and individuals with low economic status). Perceptions on psychological disturbances were themed as consequences of social distancing, management of dead bodies, exhaustion among the health care workers, and risk of exposure. Finally, lessons learned were centered around building on the existing epidemiological and public health capacities, improving access to health care and overcoming resistance to change. Most participants labelled their experience in COVID-19 as an “experience of wisdom” in which learning was a continuous process. Conclusion This qualitative study amongst primary HCWs revealed certain aspects of response to COVID-19 in Muscat, Oman. Results has unfolded various aspects of COVID-19. The situation was perceived by primary HCWs as a new experience that challenged the primary health care; enforced the utilization of public health/epidemiological skills, and linked to unfavorable socio-religious and psychological events.
Biomechanics of Ankle Ligament Reconstruction: A Cadaveric Study to Compare Stability of Reconstruction Techniques Using 1 or 2 Fibular Tunnels
Authors: Gautschi Martina , Bachmann Elias , Shirota Camila , Götschi Tobias , Renner Niklas , Wirth Stephan H. 
Published: October 2020

Background: Anatomic lateral ankle ligament reconstruction has been proposed for patients with chronic ankle instability. A reliable approach is a reconstruction technique using an allograft and 2 fibular tunnels. A recently introduced approach that entails 1–fibular tunnel reconstruction might reduce the risk of intraoperative complications and ultimately improve patient outcome. Hypothesis: We hypothesized that both reconstruction techniques show similar ankle stability (joint laxity and stiffness) and are similar to the intact joint condition. Study Design: Controlled laboratory study. Methods: A total of 10 Thiel-conserved cadaveric ankles were divided into 2 groups and tested in 3 stages—intact, transected, and reconstructed lateral ankle ligaments—using either the 1– or the 2–fibular tunnel technique. To quantify stability in each stage, anterior drawer and talar tilt tests were performed in 0°, 10°, and 20° of plantarflexion (anterior drawer test) or dorsiflexion (talar tilt test). Bone displacements were measured using motion capture, from which laxity and stiffness were calculated together with applied forces. Finally, reconstructed ligaments were tested to failure in neutral position with a maximal applicable torque in inversion. A mixed linear model was used to describe and compare the outcomes. Results: When ankle stability of intact and reconstructed ligaments was compared, no significant difference was found between reconstruction techniques for any flexion angle. Also, no significant difference was found when the maximal applicable torque of the 1-tunnel technique (9.1 ± 4.4 N·m) was compared with the 2-tunnel technique (8.9 ± 4.8 N·m). Conclusion: Lateral ankle ligament reconstruction with an allograft using 1 fibular tunnel demonstrated similar biomechanical stability to the 2-tunnel approach. Clinical Relevance: Demonstrating similar stability in a cadaveric study and given the potential to reduce intraoperative complications, the 1–fibular tunnel approach should be considered a viable option for the surgical therapy of chronic ankle instability. Clinical randomized prospective trials are needed to determine the clinical outcome of the 1-tunnel approach.
Clinician Word Use in Dementia Evaluation Reports as a Function of Cognitive Impairment
Authors: Flaherty Lauren B. , Mast Benjamin T. 
Published: October 2020

Objectives: To examine the extent to which levels of cognitive status influence patterns of word use in dementia evaluation reports. Methods: We utilized neuropsychological evaluation reports from 61 geriatric primary care patients referred for suspected dementia. Linguistic Inquiry Word Count analysis was utilized to examine clinician language use in patient reports and whether language use differs dependent on the diagnosis rendered. ANOVA analyses were used to analyze group differences in LIWC word counts across clinical indices of cognitive functioning: dementia diagnosis. Results: Our analysis revealed significant differences in language use across diagnostic categories. ANOVA analyses yielded differences in broad negative emotion, F(2,58) = 4.010, p = .023 as well as other subgroups; anxiety-related word groups, F(2,58) = 4.706, p = .013; insight words, F(2,58) = 3.815, p = .028; causation words, F(2,58) = 3.497, p = .037; certainty words, F(2,58) = 6.581, p = .003; negation words, F(2,58) = 3.165, p = .05; time-related words; F(2, 58) = 7.521, p < .001; and human-related words, F(2,58) = 6.512, p = .003. Conclusion: The differences in clinician language use across different diagnostic groups may be reflections of implicit emotional reactions. Many of the patterns found in this study can be linked to previous research concerning word use and underlying thought processes. Clinical Implications: Awareness of language use is helpful in clinical relationships to attenuate stigma and facilitate treatment and research.
The mineralogy of the historical Mochalin Log REE deposit, South Urals, Russia. Part III. Percleveite-(La), La2Si2O7, a new REE disilicate mineral
Authors: Kasatkin Anatoly V. , Zubkova Natalia V. , Pekov Igor V. , Chukanov Nikita V. , Škoda Radek , Agakhanov Atali A. , Belakovskiy Dmitriy I. , Ksenofontov Dmitriy A. , Plášil Jakub , Kuznetsov Aleksey M. , Britvin Sergey N. , Pushcharovsky Dmitry Yu. 
Published: October 2020

AbstractThe new mineral percleveite-(La) (IMA2019–037), ideally La2Si2O7, was found in polymineralic nodules of the Mochalin Log REE deposit, Chelyabinsk Oblast, South Urals, Russia. It is associated with allanite-(Ce), allanite-(La), bastnäsite-(Ce), bastnäsite-(La), ferriallanite-(Ce), ferriallanite-(La), ferriperbøeite-(Ce), ferriperbøeite-(La), fluorbritholite-(Ce), hydroxylbastnäsite-(Ce), perbøeite-(Ce), perbøeite-(La), törnebohmite-(Ce) and törnebohmite-(La). Percleveite-(La) occurs as isolated anhedral grains commonly up to 0.2 mm × 0.4 mm and very rarely up to 1 mm × 1 mm. The new mineral is transparent with greasy lustre. The mineral is very pale yellow to colourless in thin fragments to light yellow in aggregates. It is brittle, with imperfect {001} cleavage and an uneven fracture. Mohs’ hardness is ca. 6. Dcalc = 5.094 g cm–3. Under the microscope, percleveite-(La) is non-pleochroic, optically uniaxial (+), ω = 1.825(10) and ɛ = 1.835(10). The Raman spectrum is given. Chemical data (wt.%, electron-microprobe) are: La2O3 36.80, Ce2O3 31.22, Pr2O3 1.57, Nd2O3 2.96, SiO2 26.73, total 99.28. The empirical formula based on 7 O apfu is (La1.02Ce0.86Nd0.08Pr0.04)Σ2.00Si2.00O7. Percleveite-(La) is tetragonal, P41; the unit-cell parameters are: a = 6.8482(3), c = 24.8550(13) Å, V = 1165.64(11) Å3 and Z = 8. The strongest reflections in the powder X-ray diffraction pattern [d, Å(I)(hkl)] are: 4.194(18)(113), 3.564(16)(106), 3.349(16)(201,202), 3.157(100)(203,116,008), 3.043(22)(211), 2.934(39)(122), 2.893(29)(213) and 2.864(21)(117). The crystal structure of percleveite-(La) is solved from the single-crystal X-ray diffraction data [R = 0.0617 for 2831 unique reflections with I > 2σ(I)]. The new mineral is named as an analogue of percleveite-(Ce) with La predominance over the rare-earth elements.
The management of mild asthma
Authors: O'Byrne Paul M. , Reddel Helen K. , Beasley Richard 
Published: October 2020

Inhaled corticosteroids (ICSs) have been recommended as a maintenance treatment, either alone or together with long-acting inhaled β2-agonists, for all asthma patients. Short-acting β2-agonists (SABAs) are rapid-onset bronchodilators, which provide symptom relief, but have no anti-inflammatory properties, yet are the most widely used as-needed reliever treatment for asthma and often the only treatment prescribed. Asthma patients can find adhering to daily preventative medication with ICS difficult and will often revert to using as-needed SABA as their only treatment, increasing their risk of exacerbations. The purpose of this review is to evaluate the efficacy of reliever medications that contain ICS compared with SABA as reliever, or with maintenance ICS and SABA as reliever, in mild asthma patients.Nine studies were identified that have evaluated the use of ICS as a component of an as-needed reliever in patients with mild asthma. Four of the most recent studies compared the combination of ICS/formoterol to SABA as reliever.ICS-containing reliever medication was superior to SABA as reliever alone, and was equivalent to maintenance ICS and SABA as reliever, particularly in reducing risks of severe asthma exacerbations, in studies which compared these reliever options.SABAs should not be used as a reliever without ICS. The concern about patients with mild asthma not being adherent to maintenance ICS supports a recommendation that ICS/formoterol should be considered as a treatment option instead of maintenance ICS, to avoid the risk of patients reverting to SABA alone.
Lung cancer in combined pulmonary fibrosis and emphysema: a large retrospective cohort analysis
Authors: Nasim Faria , Moua Teng 
Published: October 2020

BackgroundCombined pulmonary fibrosis and emphysema (CPFE) is characterised by upper lobe emphysema and lower lobe fibrosis. Our study aim was to determine the incident risk, presenting characteristics and outcome of lung cancer diagnoses in a cohort of CPFE patients over time.Materials and methodsWe conducted a retrospective cohort study assessing patients with radiological CPFE followed over a median of 76 months (range 1–237 months). Interval development of lung cancer and clinicopathological characteristics of those with and without lung cancer were compared and survival analysis performed.ResultsLung cancer occurred in 26 (11.6%) out of 230 CPFE patients, dominated by nonsmall cell lung cancer (88%, n=23) with squamous cell carcinoma comprising the majority (57%, n=13). There was a predominance of lower lobe (62%) and subpleural (64%) radiological presentation. Survival was reduced for the whole cohort by lung cancer even after adjusting fora prioricovariables of age, sex, smoking pack-years, presenting forced vital capacity and radiological honeycombing. Univariable predictors of increased mortality after lung cancer diagnosis included honeycombing (hazard ratio (HR) 3.03, 95% CI 1.16–7.91; p=0.02) and later stage presentation (HR 4.77, 95% CI 1.8–14.94; p=0.001), with those able to undergo surgical resection having better survival (HR 0.29, 95% CI 0.09–0.87; p=0.02).ConclusionLung cancer occurred in 26 (11.6%) out of 230 CPFE patients and was dominated by squamous cell carcinoma presenting in a lower lobe peripheral distribution. Surgical resection appeared to improve survival in selected patients with earlier stage disease. Further studies are needed to develop a relevant screening programme for CPFE patients.
Chest radiography is a poor predictor of respiratory symptoms and functional impairment in survivors of severe COVID-19 pneumonia
Authors: D'Cruz Rebecca F. , Waller Michael D. , Perrin Felicity , Periselneris Jimstan , Norton Sam , Smith Laura-Jane , Patrick Tanya , Walder David , Heitmann Amadea , Lee Kai , Madula Rajiv , McNulty William , Macedo Patricia , Lyall Rebecca , Warwick Geoffrey , Galloway James B. , Birring Surinder S. , Patel Amit , Patel Irem , Jolley Caroline J. 
Published: October 2020

BackgroundA standardised approach to assessing COVID-19 survivors has not been established, largely due to the paucity of data on medium- and long-term sequelae. Interval chest radiography is recommended following community-acquired pneumonia; however, its utility in monitoring recovery from COVID-19 pneumonia remains unclear.MethodsThis was a prospective single-centre observational cohort study. Patients hospitalised with severe COVID-19 pneumonia (admission duration ≥48 h and oxygen requirement ≥40% or critical care admission) underwent face-to-face assessment at 4–6 weeks post-discharge. The primary outcome was radiological resolution of COVID-19 pneumonitis (Radiographic Assessment of Lung Oedema score <5). Secondary outcomes included clinical outcomes, symptom questionnaires, mental health screening (Trauma Screening Questionnaire, seven-item Generalised Anxiety Disorder assessment and nine-item Patient Health Questionnaire) and physiological testing (4-m gait speed (4MGS) and 1-min Sit-to-Stand (STS) tests).Results119 patients were assessed between June 3, 2020 and July 2, 2020 at median (interquartile range (IQR)) 61 (51–67) days post-discharge: mean±sdage 58.7±14.4 years, median (IQR) body mass index 30.0 (25.9–35.2) kg·m−2, 62% male and 70% ethnic minority. Despite radiographic resolution of pulmonary infiltrates in 87%, modified Medical Research Council Dyspnoea (breathlessness) scale grades were above pre-COVID-19 baseline in 44%, and patients reported persistent fatigue (68%), sleep disturbance (57%) and breathlessness (32%). Screening thresholds were breached for post-traumatic stress disorder (25%), anxiety (22%) and depression (18%). 4MGS was slow (<0.8 m·s−1) in 38% and 35% desaturated by ≥4% during the STS test. Of 56 thoracic computed tomography scans performed, 75% demonstrated COVID-19-related interstitial and/or airways disease.ConclusionsPersistent symptoms, adverse mental health outcomes and physiological impairment are common 2 months after severe COVID-19 pneumonia. Follow-up chest radiography is a poor marker of recovery; therefore, holistic face-to-face assessment is recommended to facilitate early recognition and management of post-COVID-19 sequelae.
Amyloid-β peptides slightly affect lifespan or antimicrobial peptide gene expression in Drosophila melanogaster
Authors: Shaposhnikov Mikhail V. , Zemskaya Nadezhda V. , Koval Lyubov А. , Minnikhanova Natalya R. , Kechko Olga I. , Mitkevich Vladimir A. , Makarov Alexander A. , Moskalev Alexey А. 
Published: October 2020

Abstract
Background
Beta-amyloid peptide (Aβ) is the key protein in the pathogenesis of Alzheimer’s disease, the most common age-related neurodegenerative disorder in humans. Aβ peptide induced pathological phenotypes in different model organisms include neurodegeneration and lifespan decrease. However, recent experimental evidence suggests that Aβ may utilize oligomerization and fibrillization to function as an antimicrobial peptide (AMP), and protect the host from infections. We used the power of Drosophila model to study mechanisms underlying a dual role for Aβ peptides.

Results
We investigated the effects of Drosophila treatment with three Aβ42 peptide isoforms, which differ in their ability to form oligomers and aggregates on the lifespan, locomotor activity and AMP genes expression. Aβ42 slightly decreased female’s median lifespan (by 4.5%), but the effect was not related to the toxicity of peptide isoform. The lifespan and relative levels of AMP gene expression in male flies as well as locomotor activity in both sexes were largely unaffected by Aβ42 peptide treatment. Regardless of the effects on lifespan, Aβ42 peptide treatment induced decrease in AMP genes expression in females, but the effects were not robust.

Conclusions
The results demonstrate that chronic treatment with Aβ42 peptides does not drastically affect fly aging or immunity.
Analyzing a putative enhancer of optic disc morphology
Authors: Babenko Vladimir , Babenko Roman , Orlov Yuri 
Published: October 2020

Abstract
Background
Genome-wide association studies have identified the CDC7-TGFBR3 intergenic region on chromosome 1 to be strongly associated with optic disc area size. The mechanism of its function remained unclear until new data on eQTL markers emerged from the Genotype-Tissue Expression project. The target region was found to contain a strong silencer of the distal (800 kb) Transcription Factor (TF) gene GFI1 (Growth Factor Independent Transcription Repressor 1) specifically in neuroendocrine cells (pituitary gland). GFI1 has also been reported to be involved in the development of sensory neurons and hematopoiesis. Therefore, GFI1, being a developmental gene, is likely to affect optic disc area size by altering the expression of the associated genes via long-range interactions.

Results
Distribution of haplotypes in the putative enhancer region has been assessed using the data on four continental supergroups generated by the 1000 Genomes Project. The East Asian (EAS) populations were shown to manifest a highly homogenous unimodal haplotype distribution pattern within the region with the major haplotype occurring with the frequency of 0.9. Another European specific haplotype was observed with the frequency of 0.21. The major haplotype appears to be involved in silencing GFI1repressor gene expression, which might be the cause of increased optic disc area characteristic of the EAS populations.
The enhancer/eQTL region overlaps AluJo element, which implies that this particular regulatory element is primate-specific and confined to few tissues.

Conclusion
Population specific distribution of GFI1 enhancer alleles may predispose certain ethnic groups to glaucoma.
Molecular and cytological analysis of widely-used Gal4 driver lines for Drosophila neurobiology
Authors: Ogienko Anna A. , Andreyeva Evgeniya N. , Omelina Evgeniya S. , Oshchepkova Anastasiya L. , Pindyurin Alexey V. 
Published: October 2020

AbstractBackgroundTheDrosophilacentral nervous system (CNS) is a convenient model system for the study of the molecular mechanisms of conserved neurobiological processes. The manipulation of gene activity in specific cell types and subtypes of theDrosophilaCNS is frequently achieved by employing the binary Gal4/UAS system. However, many Gal4 driver lines available from the Bloomington Drosophila Stock Center (BDSC) and commonly used inDrosophilaneurobiology are still not well characterized. Among these are three lines with Gal4 driven by theelavpromoter (BDSC #8760, #8765, and #458), one line with Gal4 driven by therepopromoter (BDSC #7415), and the 69B-Gal4 line (BDSC #1774). For most of these lines, the exact insertion sites of the transgenes and the detailed expression patterns of Gal4 are not known. This study is aimed at filling these gaps.ResultsWe have mapped the genomic location of the Gal4-bearingP-elements carried by the BDSC lines #8760, #8765, #458, #7415, and #1774. In addition, for each of these lines, we have analyzed the Gal4-driven GFP expression pattern in the third instar larval CNS and eye-antennal imaginal discs. Localizations of the endogenous Elav and Repo proteins were used as markers of neuronal and glial cells, respectively.ConclusionsWe provide a mini-atlas of the spatial activity of Gal4 drivers that are widely used for the expression of UAS–target genes in theDrosophilaCNS. The data will be helpful for planning experiments with these drivers and for the correct interpretation of the results.