bluuebunny/crossref_metadata_2025_split huggingface dataset:
PURIFICATION, CHARACTERIZATION AND EXPRESSION, OF RECOMBINANT OUTER MEMBRANE PROTEIN A (OMP A) OF ACINETOBACTER BAUMANNII LI311
Authors: Al-Saadi Aamal Ghazi , Abbas Aalaa Fahim 
Published: October 2020

Acinetobacter baumannii is a known hospital aquired pathogenic bacterium that increasingly resists antibiotics treatment. In order to characterize and produce a soluble OmpA protein that can be used to develop Acinetobacter vaccine, polymerase chain reaction (PCR) was used to produce the ompA gene, of A. baumannii strain LI311, which was cloned into the histidin taged pET19b expression plasmid. Immobilized metal affinity chromatography (IMAC) was utilized to purify the recombinant protein, and amino acid sequences for OmpA protein homologs were attained from the National Center for Biotechnology Information (NCBI) protein resource then analyzed using the blast tool and Jalview program. Protein topology prediction was done using NCBI tools and PRED-TMBB2. Analysis of amino acid sequence of OmpA of A. baumannii strain LI311 showed that it has homologies to other clinical Acinetobacter spices , including: A. pittii , A.nosocomialis , A.seifertii, A. calcoaceticus, and A. ursingii with identity percentages of 100%, 100%, 96%, 92%, and 91%. Protein topology prediction revealed two conserved domains belonging to OmpA family protein ,which are beta-barrel domain outer membrane protein (OMP_b-brl) and OmpA-C-like domain, and it is a 10-βeta -stranded transmembrane Outer Membrane Protein with a signal peptide at residues 1–22A. A recombinant Histidine tagged- OmpA (39.31kDa )was successfully expressed and purified in this study. In conclusion, OmpA protein of A.baumannii strain LI31 is highly conserved across clinical species of Acinetobacter, and the soluble recombinant OmpA created in this study can be used to develop a putative vaccine that may prevent infections caused by the clinical species of Acinetobacter.
Spatiotemporal ATP Dynamics during AKI Predict Renal Prognosis
Authors: Yamamoto Shinya , Yamamoto Masamichi , Nakamura Jin , Mii Akiko , Yamamoto Shigenori , Takahashi Masahiro , Kaneko Keiichi , Uchino Eiichiro , Sato Yuki , Fukuma Shingo , Imamura Hiromi , Matsuda Michiyuki , Yanagita Motoko 
Published: October 2020

Significance Statement
ATP depletion is central in kidney disease pathogenesis, but technology has not effectively monitored in vivo ATP dynamics. Experiments using a novel mouse strain that incorporates an ATP biosensor to visualize spatiotemporal ATP dynamics at single-cell resolution revealed the crucial difference between cells of the proximal tubules (PTs) and those of the distal tubules (DTs). Mitochondrial changes were consistent with the differing ATP dynamics in cells of the two regions, explaining the different sensitivity to ischemic reperfusion injury. A strong correlation emerged between ATP recovery of PT cells in acute phase and renal fibrosis in the chronic phase. Cold ischemia enhanced ATP recovery, providing a proof of concept for the possible protective value of renal hypothermia.

Background
Depletion of ATP in renal tubular cells plays the central role in the pathogenesis of kidney diseases. Nevertheless, inability to visualize spatiotemporal in vivo ATP distribution and dynamics has hindered further analysis.

Methods
A novel mouse line systemically expressing an ATP biosensor (an ATP synthase subunit and two fluorophores) revealed spatiotemporal ATP dynamics at single-cell resolution during warm and cold ischemic reperfusion (IR) with two-photon microscopy. This experimental system enabled quantification of fibrosis 2 weeks after IR and assessment of the relationship between the ATP recovery in acute phase and fibrosis in chronic phase.

Results
Upon ischemia induction, the ATP levels of proximal tubule (PT) cells decreased to the nadir within a few minutes, whereas those of distal tubule (DT) cells decreased gradually up to 1 hour. Upon reperfusion, the recovery rate of ATP in PTs was slower with longer ischemia. In stark contrast, ATP in DTs was quickly rebounded irrespective of ischemia duration. Morphologic changes of mitochondria in the acute phase support the observation of different ATP dynamics in the two segments. Furthermore, slow and incomplete ATP recovery of PTs in the acute phase inversely correlated with fibrosis in the chronic phase. Ischemia under conditions of hypothermia resulted in more rapid and complete ATP recovery with less fibrosis, providing a proof of concept for use of hypothermia to protect kidney tissues.

Conclusions
Visualizing spatiotemporal ATP dynamics during IR injury revealed higher sensitivity of PT cells to ischemia compared with DT cells in terms of energy metabolism. The ATP dynamics of PTs in AKI might provide prognostic information.
Occupational catastrophe! The digital revolution, global warming, unrest and pandemics: are we prepared?
Authors: Joubert Robin Wendy 
Published: October 2020

Through an extensive scrutiny of relevant print and online media, research publications, global reports and against a participatory occupational justice framework (POJF), the author explores 4 phenomena that are increasingly impacting upon human occupation. They are the 4th industrial revolution with its accelerated technological advancement; global warming and the concomitant climate changes that are collectively causing human and agricultural displacement with catastrophic side effects; global unrest which is resulting in the displacement and disruption of large numbers of people and the corona virus pandemic with its emerging effects upon so many aspects of human existence.This is an opinion piece in which the author proposes a potential scenario that will change the face of human occupation and contribute towards considerable threat to the health and wellness of humanity. A scenario in which many occupations will be replaced by advanced technology and/or activities of daily living transformed by climate change. This, together with human displacement, will result in millions of people being jobless and the profile of human occupation changing. The impact of the corona virus remains an emerging phenomenon that is and will continue to have far reaching effects upon human behaviour, occupation, health and the global economy.A further consequence of all these phenomena will provide the world with spiritual, ethical and moral dilemmas that threaten the training and capabilities of health professionals.As health professionals with a profound understanding of the physical, mental and spiritual importance of human occupation uponthe health of humanity, occupational therapists will need to decide whether or not they are going to react creatively and proactively to these challenges or remain within the boxes of their past.
Guided by the collaborative ethos and enablement skills of the Participatory Occupational Justice Framework (POJF), and current proactive movements within the WFOT and occupational science, the author suggests a tentative approach the occupational therapy profession can adopt in order to contribute to the continuing wellness of humanity and how it may need to transform in order to prepare itself to accost and overcome these challenges.
Key words: Digital revolution, global warming and climate change, global unrest, Pandemics, human displacement, unemployment, occupationalchaos and transformation
PHYTOADAPTOGENS IN THE TUMOURS BIOTHERAPY AND GERIATRICS (PART 2)
Authors: Bocharova O. A. , Karpova R. V. , Bocharov E. V. , Vershinskaya A. A. , Baryshnikova M. A. , Kazeev I. V. , Kucheryanu V. G. , Kiselevskiy M. V. 
Published: October 2020

Phytoadaptogens (ginseng, eleutherococcus, rhodiola rosea, aralia, etc.) have a wide spectrum of biological activity, supporting the body in a state of nonspecific increased resistance to stress. The second part of the review provides information of the phytoadaptogens normalizing effects on the cardiovascular system. Analyzes current data on the antitumor properties of phytoadaptogens in relation to the effect on the onset and development of tumours, metastasis, recurrence, development of cytostatic disease, confirming the prospect of their using in drugs for prevention and biotherapy of tumour diseases.Past 1 see: Phytoadaptogens in the tumours biotherapy and geriatrics (Past 1). Russian Journal of Biotherapy 2020;19(2):13–21.
Accuracy of Near-Fault Fling-Step Displacements Estimated Using the Discrete Wavenumber Method
Authors: Wu Shuang-Lan , Nozu Atsushi , Nagasaka Yosuke 
Published: October 2020

ABSTRACTNear-fault fling steps might cause severe damage to near-fault structures such as bridges or base-isolated buildings. Therefore, the accurate simulation of ground displacements including fling steps based on fault models is an important issue not only for seismological but also for engineering purposes. The discrete wavenumber (DWN) method (e.g., Bouchon, 2003) has been established as a method to calculate complete elastic wavefield, including permanent displacement for a homogeneous or a layered half-space. However, the accuracy of the permanent displacements calculated by the DWN method is influenced by the selection of parameters, such as the imaginary part of the complex frequency and the subfault size in the case of extended sources. The objective of this study is to clarify the requirement for these parameters for the accurate simulation of fling-step displacements to further enhance the use of the DWN method. Honda and Yomogida (2003) also addressed the issue of calculating fling-step displacements using the DWN method; however, their study was focused on cases in which a large amount of seismic moment is released at depth. This study was focused on fling-step displacements due to rather shallow slip, in which the fault distance was as small as several meters in an extreme case, motivated by recent damaging earthquakes such as the 2016 Kumamoto, Japan, earthquakes. The ground displacements including fling steps were calculated by the DWN method and compared with the analytical solutions for the static displacements (Okada, 1985, 1992), both for point sources and extended sources in a homogeneous half-space. According to the results, following recommendations were made. For the imaginary part of the complex frequency, ωc=ω−λi, λ=ξπ/Tw with ξ≥2.0 can be recommended, with the understanding that the waveforms are effective only within the range of [0,Tw/ξ]. For extended sources, the subfault size should be as small as 0.5 times the fault distance to accurately simulate fling steps.
Demographics and age changes in geriatric population- part 1
Authors: Prakash Poonam , K Bhandari S 
Published: October 2020

With the improved health care facilities and nutritional levels, the average life expectancy of Indian population has been on a steady rise over the years. Geriatrics, or geriatric medicine, is a specialty that focuses on health care of elderly people. It aims to promote the health by preventing and treating diseases and disabilities in older adults. There is no set age at which patients may be under the care of a geriatrician, or geriatric physician, a physician who specializes in the care of elderly people. Rather, this decision is determined by the individual patient's needs, and the availability of a specialist. It is important to note the difference between geriatrics, the care of aged people, and gerontology, which is the study of the ageing process itself. The term geriatrics comes from the Greek γέρων geron meaning "old man", and ιατρός iatros meaning "healer". However, geriatrics is sometimes called medical gerontology. It is also defined as the branch of medical science dealing with old age & its disease. The increased life expectancy demands an improved quality of life for the elderly thereby making geriatric medicine and dentistry an important aspect of the healthcare system.This paper covers the overall demographics of the elderly population along with the various physical, dental and psychological changes that occur in them.
AKTIVITAS ANTIOKSIDAN HIDROLISAT PROTEIN IKAN WADER (Rasbora jacobsoni) DARI HIDROLISIS OLEH ENZIM CALOTROPIN DAN PAPAIN
Authors: Witono Yuli , Maryanto Maryanto , Taruna Iwan , Masahid Ardiyan Dwi , Cahyaningati Kinanti 
Published: October 2020

The potency of common barb fish as fish protein hydrolyzate caused by its high protein content. Fish protein hydrolyzate is produced through the hydrolysis process by the calotropin enzyme and the papain enzyme. This study used common barb fish with one type of treatment, i.e. the difference combination of the calotropin (C) and papain (P) enzyme. Making fish protein hydrolyzate is used to determine the antioxidant activity of the common barb fish protein hydrolyzate. The observation parameters in this study were enzyme activity, antioxidant activity, degree of hydrolysis, molecular weight, amino acids, dissolved protein content, and water holding capacity. Samples with a ratio of 40C: 60P enzyme concentration are wader fish protein hydrolyzate with the proportion of 40% calotropin enzyme and 60% papain enzyme is the highest sample that has an antioxidant activity of 36.41%, reduced power value of 0.57, hydrolysis degree of 74.40% and total amino acids of 82.79%. Wader fish hydrolyzate in the 70C: 30P sample of 76.98% has a high water holding capacity because it can maintain the protein component in meat.
Keywords: antioxidant, calotropin, common barb fish, fish protein hydrolyzate, papain
Quality of Life and mortality in the general population: a systematic review and meta-analysis
Authors: Phyo Aung Zaw Zaw , Freak-Poli Rosanne , Craig Heather , Gasevic Danijela , Stocks Nigel , Gonzalez-Chica David A. , Ryan Joanne 
Published: October 2020

Abstract
Background: Quality of life (QoL) is multi-dimensional concept of an individual’ general well-being status in relation to their value, environment, cultural and social context in which they live. This study aimed to quantitatively synthesise available evidence on the association between QoL and mortality in the general population.Methods: An electronic search was conducted using three bibliographic databases, MEDLINE, EMBASE and PsycINFO. Inclusion criteria were studies that assessed QoL using standardized tools and examined mortality risk in a non-patient population. Qualitative data synthesis and meta-analyses using a random-effects model were performed.Results: Of 4,184 articles identified, 47 were eligible for inclusion, involving approximately 1,200,000 participants. Studies were highly heterogeneous in terms of QoL measures, population characteristics and data analysis. In total, 43 studies (91.5%) reported that better QoL was associated with lower mortality risk. The results of four meta-analyses indicated that higher health-related QoL (HRQoL) is associated with lower mortality risk, which was consistent for overall HRQoL (HR 0.633, 95% CI: 0.514 to 0.780), physical function (HR 0.987, 95% CI: 0.982 to 0.992), physical component score (OR 0.950, 95% CI: 0.935 to 0.965), and mental component score (OR 0.980, 95% CI: 0.969 to 0.992). Conclusion: These findings provide evidence that better QoL/HRQoL was associated with lower mortality risk. The utility of these measures in predicting mortality risk indicates that they should be considered further as potential screening tools in general clinical practice, beyond the traditional objective measures such as body mass index and the results of laboratory tests.
Feature-driven classification reveals potential comorbid subtypes within childhood apraxia of speech
Authors: Stein Catherine , Benchek Penelope , Miller Gabrielle , Hall Noémi B. , Menon Dhanya , Freebairn Lisa , Tag Jessica , Vick Jennell , Taylor H. Gerry , Lewis Barbara A. , Iyengar Sudha K. 
Published: October 2020

Abstract
Background

Childhood apraxia of speech (CAS) is a neurodevelopmental disorder with heterogeneous communication and other comorbid manifestations. While previous studies have characterized speech deficits associated with CAS, few studies have examined variability in reading and language and/or other developmental comorbidities. We sought to identify comorbid subgroups within CAS that could be clinically relevant as well as genetically distinctive.

Methods

In a group of 31 children with CAS and 8 controls, we performed hierarchical cluster analysis utilizing measures of articulation, vocabulary, and reading. We also conducted a chart review of the children with CAS to examine other clinical characteristics in these children and their association with subgroup membership.

Results

We identified 3 comorbid subgroups within CAS of varying severity. The high severity subgroup was characterized by poor reading and vocabulary, and the moderate severity subgroup by poor reading and non-word repetition but average vocabulary, compared to the mild severity subgroup. Subgroups were indistinguishable with respect to speech sound production, the hallmark of CAS, all demonstrating poor articulation. Children in the most severe subgroup were more likely to have early problems feeding (p=0.036).

Conclusions

Children with CAS may potentially be classified into comorbidity groups based on performance on vocabulary and reading measures, providing additional insight into the heterogeneity within CAS with implications for educational interventions.
Trends of multimorbidity in 15 European countries: a population-based study in community-dwelling adults aged 50 and over
Authors: Souza Dyego L. B. , Oliveras-Fabregas Albert , Minobes-Molina Eduard , Cancela Marianna de Camargo , Galbany-Estragués Paola , Roig Javier Jerez 
Published: October 2020

Abstract
Background: The objective of this work was to analyse the prevalence trends of multimorbidity among European community-dwelling adults.Methods: A temporal series study based on waves 1, 2, 4, 5, 6 and 7 of the Survey of Health, Ageing and Retirement in Europe (SHARE) was conducted, and community-dwelling participants aged 50+ (n=274,614) from 15 European countries were selected for the period 2004-2017. Prevalence, adjusted by age, Average Annual Percentage Change (APC) and 95% confidence interval (95% CI) were all calculated. Trend analyses were realised by period, age groups and groups of diseases.Results: The results showed a large variability in the prevalence of multimorbidity in adults aged 50 and over among European countries. Increase in the prevalence of multimorbidity in the countries of central Europe (Austria, Belgium, Czech Republic, France, Germany and Switzerland) and Spain in both sexes, and in the Netherlands among men. Stability was observed in northern and eastern European countries. Musculoskeletal and neurodegenerative groups showed more significant changes in the trend analyses.Conclusions: This information can be useful for policy makers when planning health promotion and prevention policies addressing modifiable risk factors in health.
Comparing the reliability and validity of the SF-36 and SF-12 in measuring quality of life among adolescents in China: a large sample cross-sectional study
Authors: Lin Yanwei , Yu Yulan , Zeng Jiayong , Zhao Xudong , Wan Chonghua 
Published: October 2020

Abstract
Objective: We compare the reliability and validity of the Short Form 36 (version 1, SF-36) and the Short Form 12 (version 1, SF-12) in adolescence, the period of life when a child develops into an adult, i.e., the period from puberty to maturity terminating legally at the age of majority (10-19 years), thus supplying evidence for the selection of instruments measuring the quality of life (QOL) and decision-making processes of adolescents in China. Methods: Stratified cluster random sampling was adopted according to geographical location, and the SF-36 was administered to assess QOL. The Pearson correlation coefficient was used to show correlation. Cronbach’s alpha and construct reliability (CR) were used to evaluate the reliability of SF-36 and SF-12, while criterion validity and average variance extracted (AVE, convergence validity) were used to evaluate validity. Confirmatory factor analysis was used to calculate the load factors for the items of the SF-36 and SF-12, then to obtain the CR and AVE. The Semejima grade response model (logistic two-parameter module) in item response theory was used to estimate item discrimination, item difficulty, and item average information for the items of the SF-36 and SF-12. Results: 19,428 samples were included in the study. The mean age of respondents was 14.78 years (SD = 1.77). Reliability of each domain of the SF-36 was better than for the corresponding domain of the SF-12. The domains of PF, RP, BP, and GH in SF-36 had good construct reliability (CR > 0.6). The criterion validities of some domains of the SF-36 were a little higher in some corresponding dimensions of the SF-12, except for PCS. The convergence validities of the SF-12 were higher than the SF-36 in PF, RP, BP, and PCS. The items of BP, SF, RP, and VT in the SF-12 had acceptable discrimination of items that were higher than in the SF-36. The items’ average amounts of information on BP, VT, SF, RE, and MH in the SF-36 and SF-12 were poor. Conclusion: Two component (PCS and MCS) measurements of the SF-12 appeared to perform at least as well as the SF-36 in cross-sectional settings in adolescence, but the reliability and validity of the 8 domains of the SF-36 were better than those of the SF-12. Some domains, for instance SF and BP, were not suitable for adolescents or need to be studied further.