bluuebunny/crossref_metadata_2025_split huggingface dataset:
Quantitative Differences in Levels of Immunohistochemical Biomarkers between Squamous Cell Carcinoma and Adenocarcinoma of the Uterine Cervix: Implications for Treatment Outcomes after Chemoradiotherapy
Authors: Chen RUI-YUN CHEN , LIN YING-CHUN , LIANG JI-AN , Hung Yao-Chin , YEH LIAN-SHUNG , CHANG WEI-CHUN , LIN WU-CHOU , CHANG YIN-YI , Chen Shang-Wen 
Published: June 2020

Abstract
Introduction: This study compared the quantitative differences in immunohistochemical markers between uterine cervical squamous cell carcinoma (SCC) and adenocarcinoma (AC) and assessed the impact of these biomarkers on outcomes in patients treated with chemoradiotherapy (CRT).Method: This retrospective study included 118 patients (SCC in 76, AC in 42) who received definitive CRT. According to the International Federation of Gynecology and Obstetrics staging system, 14, 34, and 70 patients were classified as having stage IB3, II, and III disease, respectively. Baseline immunohistochemical biomarkers, including hypoxia, cell proliferation, cell adhesion, immunogenicity, inflammatory, and evasion of apoptosis biomarkers, were analyzed using tissue microarrays from biopsy specimens. The Mann-Whitney U test was carried out for quantitative analysis between SCC and AC. Cox regression analysis was used to examine the effects of the biomarkers and clinical parameters on treatment outcomes.Results: Using the H-scores of the biomarkers for SCC as a reference, increased expression of E-cadherin, calretinin, CAIX, and c-Myc and decreased levels of HIF-1α, VEGF, tumor necrosis factor-α, galectin-9, chemokine ligand 5, Bax, EGFR, and insulin-like growth factor 1 receptor were found in the patients with AC. A high E-cadherin (P = 0.002) and low Bax (P = 0.001) H-score were associated with inferior pelvic relapse-free survival. Conclusion: Cervical SCC exhibited strong expression of baseline immunohistochemical inflammatory, hypoxic, and angiogenesis biomarkers whereas the intensity of cell adhesion markers was more distinct in cervical AC. A high E-cadherin and a low Bax H-score were associated with a high rate of local relapse.
Contribution to a Mixed Assessment of Patient Safety Culture
Authors: Fourar Youcef Oussama , Djebabra Mebarek , Benhassine Wissal , Boubaker Leila 
Published: June 2020

Abstract
Purpose: The evaluation of patient safety culture is conducted using quantitative methods based on the use of questionnaires and qualitative ones focused on the deployment of cultural maturity models. These methods are known to suffer from certain major limits. This article aims to overcome the difficulties encountered by both methods and to propose a novel approach to the assessment of PSC. Methodology: The approach proposed in this article consists of applying a combined method, based on Principal Component Analysis (PCA) and K-means algorithm, to group together PSC dimensions into macro-dimensions whose exploitation allows to overcome the difficulties encountered with dimensional analysis of PSC and then, serve as a basic support for the development of a patient safety culture maturity model. Findings: The results of the combined method PCA / k-means shows that PSC dimensions can be grouped into three macro-dimensions that were capitalized in a first place using factors related to the development of PSC and in a second place to develop a quantitative maturity matrix that helped in the identification of PSC maturity levels.Originality: The merit of our proposal is to work towards a quali-quantitative evaluation of safety culture recommended by a good number of researchers but, to our knowledge, few or no studies are devoted to this hybrid or systematic evaluation of safety culture. Thus, the results can also be projected to implicate PSC actors and to frame the evaluation pf PSC maturity by international standards.
G6PD deficiency is associated with renal impairment in T2DM
Authors: Basnet Nabin Bahadur , Pradhan Santosh , Kafle Rishi Kumar , Gautam Keyoor 
Published: June 2020

Abstract
Background Diabetes and its complications including renal impairment are the consequences of increased oxidative stress. Glucose-6-phosphate dehydrogenase (G6PD) is responsible for protection from harmful oxidative metabolites. G6PD deficiency hence leaves these individuals at high risk of oxidative stress and at high risk of diabetes and its complications including renal impairment. If any association between G6PD deficiency and damage to kidney exists in diabetic population then screening for G6PD deficiency in this population will help identify the susceptible patient who may be at higher risk of diabetic complications due to G6PD deficiency and can prevent them with early interventions to help decrease patients’ morbidity in the long run. Methods Healthy control volunteers and diabetic with or without renal impairment were selected after counseling. Body Mass Index and blood pressure were recorded. Blood tests to measure the G6PD activity in fresh whole blood, hemoglobin, glycated hemoglobin, uric acid, and creatinine were done. Urine was tested for the presence of proteinuria. The activity of G6PD was measured. Glomerular filtration rate (eGFR) was estimated with the Modification of Diet in Renal Disease Study (MDRD) Equation. Calculations and statistical analysis were done with open software LibreOffice 6.3.3 and SPSS version 22.0.Results The mean G6PD level of healthy control was 84.27 ± 9 and that of diabetic patient without renal impairment and diabetic patients with renal impairment were 65.05 ± 5.35 and 46.64 ± 6.56 respectively. There was only one participant (4.55%) with G6PD deficiency among healthy controls whereas the proportion of participants having normal, mild to moderate, and severe degree of G6PD deficiency were 77.27%, 13.64%, and 9.09%, respectively for diabetic patient without renal impairment while 40.91%, 22.73%, and 36.36% for those with renal impairment. The proportion of participants with renal impairment had more severe degree of G6PD deficiencies than those without renal impairment (p=0.03).Conclusion The prevalence of G6PD deficiency was higher in diabetics with renal impairment than those with diabetes alone suggesting the relation between G6PD activity and diabetes with renal impairment.
An adaptive nonsingular fast terminal sliding mode control for yaw stability control of bus based on STI tire model
Authors: Sun xiaoqiang , Wang Yujun , Cai Yingfeng , Wong PakKin , Chen Long 
Published: June 2020

Abstract
In this paper, a novel adaptive nonsingular fast terminal sliding mode (ANFTSM) control scheme for yaw stability control (YSC) is proposed to improve the bus curve driving stability and safety on slippery roads. There are three major contributions in the design process of the bus YSC system. The first contribution is that the STI (Systems Technologies Inc.) tire model, which can effectively reflect the coupling relationship between the tire longitudinal force and lateral force, is established based on experimental data and firstly adopted in the bus YSC system design. The second contribution is a novel YSC strategy based on ANFTSM, which has the merits of fast transient response, finite time convergence and high robustness against uncertainties and external disturbances. The third contribution is that the robust least-squares allocation method is used to solve the optimal allocation problem of the tire forces, whose objective is to achieve the desired direct yaw moment through the effective distribution of the brake force of each tire. To verify the feasibility, effectiveness and practicality of the proposed bus YSC approach, the TruckSim-Simulink co-simulation results are finally provided.
Clinic Correlation and Prognostic Value of P4HB and GRP78 Expression in Gastric Cancer
Authors: Zhang Chenfei , Zhao Wenjing , Zhang Xingsong , Wu Tongyu , Zhang Xunlei , Song Li , Xu Weiwei , Chen Xudong , Yang Lei 
Published: June 2020

Abstract
Background: Prolyl 4-hydroxylase, beta polypeptide (P4HB) and Glucose‑regulated protein 78 (GRP78) are represent for poor prognosis of various cancers. However, rare research investigate the correlation of them in cancer. This study aimed to investigate the correlation and prognostic value of P4HB and GRP78 expression along with clinical features in gastric cancer (GC).Methods: A total of 150 GC tissue samples separately evaluated P4HB and GRP78 protein expression by immunohistochemistry. Association of P4HB and GRP78 expression with clinicopathological features was analyzed. Bioinformatics analyses were performed to find correlation between mRNAs and pathways. Kaplan-Meier analyses were taken to compare survival curves. Univariate and multivariate Cox regression models used to analyze the impact of prognostic factors on overall survival (OS). A prognostic nomogram was constructed based on the multivariate Cox regression model, and compared to TNM stage in discrimination ability and clinical usefulness.Results: P4HB and GRP78 expression were both associated with tumor invasion and lymph node metastasis. P4HB protein positively correlated with GRP78, as well as the same tendency of mRNAs in both GC tissue and cell databases. Bioinformatics analyses indicated that P4HB and GRP78 may be associated with protein folding and response to ER stress, and the protein processing in ER pathway. High single or co-expression of P4HB and GRP78 represented for a shorter OS. When stratified by postoperative adjuvant chemotherapy, the high co-expression was only related to unfavorable prognosis of with chemotherapy group, especially in advanced stage. Multivariate Cox regression analysis identified co-expression of P4HB and GRP78 as an independent prognostic factor for OS. The nomogram including the co-expression was better than TNM stage in discrimination ability and clinical usefulness through the receiver operating characteristic (ROC) and decision curve analysis (DCA) curves.Conclusion: P4HB was positively correlated with GRP78 expression, co-expression of them was an independent prognostic factor, could serve as a predictive marker for GC patients with postoperative adjuvant chemotherapy in advanced stage.
MiR-221-3p-Mediated Downregulation of MDM2 Reverses the Paclitaxel Resistance of Non-Small Cell Lung Cancer in Vitro and in Vivo
Authors: Ni Liwei , Xu Jianhao , Zhao Fenglun , Dai Xiaoxiao , Tao Jialong , Pan Jie , Shi Aiming , Shen Zhu , Su Cunjin , Zhang Yusong 
Published: June 2020

Abstract
Background: MicroRNAs (miRNAs) are involved in the initiation and development of cancer, and participate in drug resistance. Paclitaxel (PTX) is first-line chemotherapy drug for advanced non-small cell lung cancer (NSCLC). The abnormal miRNA expression in NSCLC and its association with chemotherapy drug resistance remains largely unknown. The study aimed to investigate the aberrant expression of miR-221-3p in NSCLC and to elucidate its molecular mechanisms in relation to PTX resistance.Methods: Quantitative polymerase chain reaction (qPCR) was used to examine miRNA and messenger RNA (mRNA) in NSCLC tissues and cell lines. The roles of miR-221-3p in NSCLC progression and drug resistance were assessed by Western blot analysis, colony formation assay, and CCK-8. Dual-luciferase reporter assay was conducted to evaluate the interaction between miR-221-3p and MDM2. Xenograft tumor models were established by subcutaneously injecting PTX-resistant A549 cells (A549/Taxol) to assess the effect of miR-221-3p on tumor growth. Data regarding miRNAs and target proteins from 20 NSCLC tissues and paired non-cancerous matched tissues were also obtained for correlation analysis. Results: PTX increased miR-221-3p expression, which regulated MDM2/P53 expression in the PTX-sensitive NSCLC strain (A549). Meanwhile, miR-221-3p was rarely expressed and not interfered by PTX in A549/Taxol. Dual luciferase reporter assay confirmed that miR-221-3p specifically binds to MDM2 mRNA. MiR-221-3p down-regulation reduced the sensitivity of A549 cells to PTX, whereas its up-regulation partially reversed the A549/Taxol cells resistance to PTX and increased the chemosensitivity of A549/Taxol cells to PTX in xenograft models. QPCR analysis revealed that miR-221-3p expression increased, whereas the MDM2 level decreased in NSCLC tumor tissues. Furthermore, the result of Western bolt analysis showed that P53 was lowly expressed in tumor tissues with MDM2 overexpression.Conclusions: MiR-221-3p overexpression could regulate MDM2/p53 signaling pathway to reverse the PTX resistance of NSCLC and induce apoptosis in vitro and vivo.
WITHDRAWN: The LncRNA XIST Promotes Colorectal Cancer Cell Growth Through Regulating miR-497-5p/FOXK1 Axis
Authors: Wang Nan , He Jia-Xing , Jia Guo-Zhan , Wang Ke , Zhou Shuai , Wu Tao , He Xian-Li 
Published: June 2020

Abstract

Background: Recently, accumulated numbers of studies have reported that long noncoding RNAs (lncRNAs) process an important role in tumorigenesis. As a new member found in lncRNAs, the role of lncRNA XIST (XIST) in colorectal cancer (CRC) was still elusive. The objective of this study was conducted to characterize a novel regulatory network involving XIST in CRC cells. Methods: The mRNAs of XIST, miR-497-5p, and forkhead box k1 (FOXK1) in CRC cells and tissues were detected using quantitative real-time polymerase chain reaction (qRT-PCR). And the proliferation and apoptosis of CRC cells were determined using cell counting kit-8 assay and flow cytometry. Moreover, we also detected the cell migration and invasion using Transwell assays. The relationships between XIST, miR-497-5p, and FOXK1 were predicted and then the dual-luciferase reporter assay was used to their relationships. The protein level of FOXK1 was quantitated using western blot. Results: In CRC tissues and cell lines, XIST expression was up-regulated, in which also existed miR-497-5p down-regulation and FOXK1 up-regulation. XIST knockdown suppressed CRC cell proliferation and migration as well as its invasion. Moreover, blocking the XIST expression could inhibit CRC tumor growth in vivo and the effects were antagonized by loss of miR-497-5p. miR-497-5p was identified as a sponge of XIST and also targeted FOXK1 in CRC cells. Besides, XIST silencing-mediated inhibitory activity against CRC progression reversed miR-497-5p down-regulation or FOXK1 up-regulation. Conclusion: In conclusion, XIST promotes the malignancy of colon cancer cells partly by competitively binding to miR-497-5p, which then led to increased FOXK1 expression. We conclude that targeting XIST may be a possible treatment for colon cancer.
Eight-Gene Metabolic Signature Related with Tumor-Associated Macrophages Predicting Overall Survival for Hepatocellular Carcinoma
Authors: Huo Junyu , Zang Yunjin , Dong Hongjing , Liu Xiaoqiang , He Fu , Zhang Xiao , Wu Liqun 
Published: June 2020

Abstract
Background: In recent years, the relationship between tumor associated macrophages (TAMs) and solid tumors has become a research hotspot. The study aims at exploring the close relationship of TAMs with metabolic reprogramming genes in hepatocellular carcinoma(HCC), in order to provide a new way of treatment for HCC.Materials and methods: The study selected 343 HCC patients with complete survival information(survival time >= 1month) in the Cancer Genome Atlas (TCGA) as the study objects. Kaplan-Meier survival analysis assisted in figuring out the relationship between macrophage infiltration level and overall survival (OS), and Pearson correlation test to identify metabolic reprogramming genes(MRGs) related to tumor macrophage abundance. Lasso regression algorithm were conducted on prognosis related MRGs screened by Univariate Cox regression analysis and Kaplan-Meier survival analysis to construct the riskscore, another independent cohort (including 228 HCC patients) from the International Cancer Genome Consortium (ICGC) were used for external validation regarding the prognostic signature.Results: A risk score composed of 8 metabolic genes can accurately predict the OS of training cohort(TCGA) and testing cohort(ICGC). It is important that the risk score could widely used for people with different clinical characteristics, and is an independent predictor independent of other clinical factors affecting prognosis. As expected, high-risk group exhibited an obviously higher macrophage abundance relative to low-risk group, and the risk score presented a positive relation to the expression level of three commonly used immune checkpoints(PD1,PDL1,CTLA4).Conclusion: Our study constructed and validated a novel eight‑gene signature for predicting HCC patients’ OS, which possibly contributed to making clinical treatment decisions.
Galectin-3: A Potential Biomarker of Rheumatic Diseases
Authors: Gruszewska Ewa , Cylwik Bogdan , Gińdzieńska-Sieśkiewicz Ewa , Kowal-Bielecka Otylia , Mroczko Barbara , Chrostek Lech 
Published: June 2020

Abstract
Background: The purpose of our study was to assess the serum galectin-3 level and its potential association with disease activity and severity indexes in patients with rheumatic diseases.Methods: Eighty-two patients with rheumatoid arthritis (RA), 49 patients with systemic sclerosis (SSc), and 18 patients with systemic lupus erythematosus (SLE) were enrolled in this study. The control group comprised 30 healthy controls. Galectin-3 concentration in serum was measured using immunochemical method. Results: The galectin-3 concentration were significantly elevated in the RA, SSc and SLE in comparison to the control group (P=0.000, P=0.000, P<0.001; respectively). But there were no significant differences in the serum galectin-3 levels between rheumatic diseases (H=0.395, P=0.821). In RA and SSc patients, galectin-3 positively correlated with erythrocyte sedimentation rate (R=0.332, P=0.004; R=0.384, P=0.009; respectively). The diagnostic accuracy (ACC) of galectin-3 was high in all rheumatic diseases (87.5% for RA, 83.1% for SSc, 81.1% for SLE). ROC analysis revealed that galectin-3 had an excellent diagnostic power in RA (AUC=0.911) and SSc (AUC=0.903) and very good for SLE (AUC=0.859).Conclusions: We concluded that serum galectin-3 due to the high diagnostic power can be a very good laboratory marker in RA and SSc patients and a useful tool in the diagnosis of SLE.
WITHDRAWN: Applicability of a Tool for Comprehensive Assessments: the Expanded Guide for Monitoring Child Development
Authors: Pekcici Emine Bahar Bingoler 
Published: June 2020

Abstract
Background: Pediatricians globally are in critical need of tools enabling comprehensive information about children and families while also saving face-to-face time. This study examined the applicability of the expanded version of the Guide for Monitoring Child Development (GMCD), a free-of-charge tool used in over 30 countries.The Expanded GMCD is a written tool incorporating the biopsychosocial model of healthcare delivery, bioecological theory of child development, and two World Health Organization frameworks: International Classification of Functioning, Disability and Health, and Nurturing Care. Methods: Parents of children attending Ankara University Developmental Pediatrics Division, Turkey, were given or digitally sent the Expanded GMCD to complete before their first assessment. The completeness of the responses to the four Expanded GMCD domains and factors associated with full completion were ascertained using multivariate analyses. Results: Of 480 children, 57% were boys, median age was 17.0 (IQR: 9.0-27.0) months. Completion rates were 78%, 87% and 92% for all four, at least three and at least two domains, respectively. All four domains “body structures and functioning,” “health conditions,” “activities and participation,” and “environmental factors” had completion rates >80%. The Expanded GMCD provided holistic information: 87% of children had developmental difficulties and special needs; 60% had chronic conditions requiring follow-up; over 20% of parents reported stigmatization and maternal depression; over 10% unemployment and financial difficulties, not getting enough support from friends and relatives, paternal depression; and 5% intra-family conflict. In the logistic regression analyses, parents of children ≤ 6 months more likely completed <4 domains compared to parents of older children (OR: 2.24; 95% CI: 1.25-3.72); maternal education less than high school (OR: 1.38; 95% CI: 0.87-2.20) and having multiple children (OR: 1.48; 95% CI: 0.94-2.35) were not significantly associated with the full completion of the Expanded GMCD.Conclusion:The applicability of the Expanded GMCD in this study in children with diverse health conditions and families with different educational levels implies its potential for applicability in other settings. Using the free-of-charge Expanded GMCD in healthcare delivery including telemedicine may address the gaps in the implementation of the theory, model and framework-based comprehensive tools for children and their families.
The impact of COVID-19 pandemic on pediatric rheumatology patients under immunosuppressive therapy: A single-center experience
Authors: Koker Oya , Demirkan Fatma Gul , Kayaalp Gulsah , Cakmak Figen , Tanatar Ayse , Karadag Serife Gul , Sonmez Emine , Omeroglu Rukiye , Ayaz Nuray Aktay 
Published: June 2020

Abstract
Objective: The aim of the research was to further broaden current knowledge of whether severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) disease 2019 (COVID-19) entails a risk for children with rheumatic diseases regarding immunosuppressive treatment.Methods: Telephone-survey was administered by conducting interviews with the parents. A message containing a link to the actual questionnaire was sent to their phones simultaneously. The medical records of the patients were reviewed for gathering information about demographic data, clinical follow-up, and treatments.Results: Patients who were followed up with immunosuppressive treatment (n=439) were attempted to be contacted between 1 May 2020 and 15 May 2020. The diagnostic distribution of patients who were accessible and eligible for the study was as follows; juvenile idiopathic arthritis (JIA) (n=243, 58.7%), autoinflammatory diseases (n=109, 26.3%), autoimmune connective tissue diseases (n=51, 12.3%) and vasculitis (n=11, 2.7%). In the entire cohort, the mean age was 12 ± 4.7 years, and 54.1% (n=224) of the patients were female. One patient with seronegative polyarticular JIA, previously prescribed methotrexate and receiving leflunomide during pandemic has been identified to be diagnosed with COVID-19. None of the patients, including the patient diagnosed with COVID-19, had any severe symptoms. More than half of the patients with household contacts required hospitalization as they were asymptomatic.Conclusion: Although circumstances such as compliance in social distancing policy, transmission patterns, attitude following contact may influence the results, immunosuppressive treatment does not seem to pose additional risk in terms of COVID-19.