bluuebunny/crossref_metadata_2025_split huggingface dataset:
si-SNHG5-FOXF2 inhibits TGF-β1-induced fibrosis in human primary endometrial stromal cells by the Wnt/β-catenin signalling pathway
Authors: Liu Limin , Chen Guobin , Chen Taoliang , Shi Wenjuan , Hu Haiyan , Song Kaijing , Huang Ruichun , Cai Huihua , He Yuanli 
Published: October 2020

Abstract
Background: Intrauterine adhesions (IUAs) are manifestations of endometrial fibrosis characterized by inflammation and fibrinogen aggregation in the extracellular matrix (ECM). The available therapeutic interventions for IUA are insufficiently effective in the clinical setting for postoperative adhesion recurrence and infertility problems. In this study, we investigated whether si-SNHG5-FOXF2 can serve as a molecular mechanism for the inhibition of IUA fibrosis ex vivo.Methods: FOXF2, TGF-β1 and collagen expression levels were measured by microarray sequencing analysis in three normal endometrium groups and six IUA patients. We induced primary human endometrial stromal cells (HESCs) into myofibroblasts (MFs) to develop an IUA cell model with various concentrations of TGF-β1 at various times. Downstream target genes of FOXF2 were screened by chromatin immunoprecipitation combined with whole-genome high-throughput sequencing (ChIP-seq). We investigated ECM formation, cell proliferation and Wnt/β-catenin signalling pathway-related proteins in primary HESCs with FOXF2 downregulation by quantitative reverse transcription-polymerase chain reaction (qRT-PCR), western blotting (WB), immunohistochemistry (IHC), flow cytometry, ethylenediurea (EdU) and CCK8 assays. We identified lncRNA SNHG5 as the upstream regulatory gene of FOXF2 through RNA immunoprecipitation (RIP), RNA pulldown and fluorescence in situ hybridization (FISH). Finally, we examined FOXF2 expression, ECM formation, cell proliferation and Wnt/β-catenin signalling pathway-related proteins in primary HESCs upon FOXF2 downregulation.Results: FOXF2 was highly expressed in the endometrium of patients with IUA. Treatment of primary HESCs with 10 ng/ml TGF-β1 for 72 h was found to be most effective for developing an IUA cell model. FOXF2 regulated multiple downstream target genes, including collagen, vimentin (VIM) and cyclin D2/DK4, by ChIP-seq and ChIP-PCR. FOXF2 downregulation inhibited TGF-β1-mediated primary HESC fibrosis, including ECM formation, cell proliferation and Wnt/β-catenin signalling pathway-related protein expression. We identified lncRNA SNHG5 as an upstream gene that directly regulates FOXF2 by RIP-seq, qRT-PCR, WB, and FISH. SNHG5 downregulation suppressed FOXF2 expression in the IUA cell model, resulting in synergistic repression of the Wnt/β-catenin pathway, thereby altering TGF-β1-mediated ECM aggregation in endometrial stromal cells ex vivo. Conclusions: Regulation of the Wnt/β-catenin signalling pathway and ECM formation by si-SNHG5-FOXF2 effectively inhibited the profibrotic effect of TGF-β1 on primary HESCs. This finding can provide a molecular basis for antagonizing TGF-β1-mediated fibrosis in primary HESCs.
Adapted Murine Sepsis Score: Improving The Research In Experimental Sepsis Mice Model
Authors: Sulzbacher Maicon Machado , Sulzbacher Lucas Machado , Passos Felipe Rafael , Bilibio Bruna Letícia Endl , Oliveira Kauana de , Althaus Wellington Felipe , Frizzo Matias Nunes , Ludwig Mirna Stela , Cruz Ivana Beatrice Mânica Da , Heck Thiago Gomes 
Published: October 2020

Abstract
Objective

The Murine Sepsis Score (MSS) is used to assess the severity of sepsis in rats and mice based on observational characteristics. The quantitative variables of blood glucose, body weight and temperature are predictors of severity in experimental models of sepsis. Therefore, our study sought to adapt the MSS with the same variables to indicate earlier the severity of the disease in murine models of the disease.
Results

Sepsis mice presented hypoglycemia, weight loss, and hypothermia. Therefore, these variables were included in the Adapted Murine Sepsis Score (A-MSS). The A-MASS presented 100% specificity and 87.5% sensibility been able to differentiate the early sepsis symptoms and its severity. The A-MSS allows an early and more complete diagnosis of sepsis in mice and might be considered as a procedure to improve the analysis of systemic sepsis dysfunction in murine experimental models.
Precise microwave ablation in hepatic neoplasms adjacent to high-risk structures: a prospective pilot study
Authors: Yang Liping , Li Qinying , Dong Gang , Sun Lulu , Xia Yan , Wu Longjun , Qi Gaoang , Wang Jianhua , Song Yanjun , Yu Jie , Liang Ping 
Published: October 2020

Abstract
Objective

To evaluate the safety and efficiency of ultrasound (US)-guided precise MWA assisted by artificial pleural effusion and/or ascites in hepatic neoplasms adjacent to high-risk structures based on a 3D preoperative planning system.
Methods

Twenty-five patients with hepatic neoplasms adjacent to high-risk structures were enrolled. CT images of all patients were reconstructed with 3D visualization software for preoperative planning. The puncture path and needle layout were estimated. US-guided precise MWA assisted by artificial pleural effusion and/or ascites was performed. Patients were followed by clinical and imaging examinations at 3, 6, and 12 months after the MWA. Study outcomes including complications, liver function, AFP level, and ablation lesion volumes were evaluated.
Results

Thirty-two tumors in 25 patients underwent precise MWA. Except for 4 patients with tumors near the macrovascular, 21 patients received artificial pleural effusion and/or ascites, achieving a complete separation. Based on preoperative 3D planning, patients with tumors near the diaphragmatic dome underwent administration of artificial pleural effusion and ascites, while patients with tumors near the gastrointestinal tract or gallbladder received artificial ascites. Of the 32 tumors, 30 achieved complete ablation in the first MWA session, and 29 achieved conformal ablation with a safe boundary of 0.5-1 cm. No severe complications were observed. Only one tumor exhibited local progression. Moreover, serum levels of ALT, AST and AFP were significantly decreased during the follow-up period.
Conclusion

Based on the 3D preoperative planning system, US-guided precise MWA assisted by artificial pleural effusion and ascites, is a safe and effective tool to treat hepatic neoplasms adjacent to high-risk structures.
Primary Immunodeficiencies Diseases (Pid) In Senegalese Children: A Cross Sectional Observational Study
Authors: Kane Awa , Deme-Ly Indou , Gueye Mame Sokhna , Niang Babacar , Mbaye-Dieyla Aminata , Diagne Guilaye , Coundoul Aliou Mar , Dieng Yaye Joor , Cissé Diénaba Fafa , Ba Idrissa Demba , Thiongane Aliou , Ba Abou , Nakoulima Aminata , Diouf Aminata , Keita Younoussa , Faye Pape Moctar , Fall Amadou Lamine , Sylla Assane , Diagne Ibrahima , Ndiaye Ousmane , Dièye Tandakha Ndiaye 
Published: October 2020

Abstract
Objective:

Primary immunodeficiencies are rarely related in sub-Saharan Africa. The aim of this study was to identify their different clinical patterns, to describe their biological phenotypes and to analyse treatment. A cross-sectional observational study over a period of 4 years and 2 months in 5 Senegalese paediatric departments was conducted. Children were recruited according to the recommendations of African society for immunodeficiencies (ASID) or the 10 warning signs for PID's diagnosis. Biological explorations were performed in these patients.
Results:

30 cases of PID were recorded. The sex ratio was 1:1; with a median age at diagnosis of 24 months. The most common clinical patterns were respiratory infections (18 patients) including tuberculosis pneumonia in almost one-third of cases (22%), followed by digestive and then cutaneous manifestations. This signs occurs in a specific context; as inbreeding (36, 7% of cases), death in sibling (30% of cases), or previous hospitalizations (66, 7%). Biological exploration made it possible to obtain immunological confirmation in 10 children, including one genetically confirmed. The most common category of PID was syndromic PID. Treatment was based mainly on antibiotic prophylaxis. PID are a reality in Senegal, but are certainly underestimated. Continue recruitment will enable advocacy for access to certain treatment.
Schistocytes Evaluation in Iron Deficiency: An Assessment Adopted From ICSH Nomenclature Guideline
Authors: Ratnaningsih Tri , Martani Galih R , Putri Dhia C , Sukorini Usi 
Published: October 2020

Abstract
Objective The diagnostic validity of schistocyte count in diagnosing iron deficiency in the microcytic population is critical to evaluate. The purpose of this study is to identify the correlation between schistocyte count and iron parameters, and the performance of schistocyte count in diagnosing iron deficiency in the microcytic population.Results Out of 805 general check-up participants, 65 subjects consisting of 17 males and 48 females aged 18–56 years had Mean Corpuscular Value (MCV) < 80 fL. Serum ferritin examination showed 25 patients with iron deficiency and the other 40 subjects without iron deficiency. There was a significant difference in the schistocyte count between the two groups (p < 0,001). Correlation analysis obtained a significant relationship between schistocyte count and serum ferritin (r=-0,67, p < 0,001). The Receiver Operating Characteristic (ROC) curve analysis provided Area Under the Curve (AUC) of schistocyte count is 0.827 with Youden Index (YI) 55% for a cut-off of ≥0.75%.
The efficacy of the combination of exhaled nitric oxide and blood eosinophil count in the diagnosis of asthma in China
Authors: Li Jiang-hua , Han Rui , Wang Yu-bo , Cheng Min , Chen Heng-yi , Lei Wen-hui , Li Li , Gao Chen , Zhao Na-na , Nie Nai-fu , Deng Bo , Yin Guo-qing , Huang Shuai , He Yong 
Published: October 2020

Abstract
BackgroundTests to identify reversible airflow limitation are important in asthma diagnosis, but they are time-consuming and may be difficult for patients to cooperate. We aim to evaluate the predictive value of fractional exhaled nitric oxide (FeNO) and blood eosinophil (B-Eos) count in asthma diagnosis, and to distinguish patients who could avoid reversibility testing.MethodsWe screened 7463 suspected asthma cases between January 2014 and December 2019 in Chongqing, China, and identified 2349 patients with complete FeNO, B-Eos count, and spirometry data. Of these, 824 were diagnosed with asthma via a positive bronchial-provocation or bronchodilation test.ResultsWhen FeNO and B-Eos counts were used in combination, the area under the receiver operating characteristic curve (AUC) for diagnosing asthma increased (0.768 vs. 0.745 or 0.728; both P < 0.001). The odds ratio for having asthma increased progressively with a gradual increase in FeNO or B-Eos count (both P < 0.001). Further analysis of in-series combinations of different threshold values for these biomarkers indicated that moderately elevated biomarker levels (FeNO > 40 ppb and B-Eos > 300 cells/μl) support a diagnosis of asthma because diagnostic specificity was > 95% and the positive likelihood ratio (PLR) was > 10. This conclusion was verified when selecting the data from 2017 to 2019 as the verification cohort.ConclusionThe combination of FeNO and B-Eos count can improve diagnostic efficacy for asthma. Patients with moderately elevated biomarkers (FeNO > 40 ppb and B-Eos > 300 cells/μl) could be diagnosed with asthma.
The Deficiency of Maged1 Attenuates Parkinson's Disease Progression by Inhibiting Apoptosis and Enhancing Autophagy in Mice
Authors: Wang Jie , You Wei-Yan , Ye Qing , Zhang Jia-Qi , He Chuan , Qi Cui , Liu Rui , Gao Xiang , Lu Ming , Hu Gang , Gao Jun 
Published: October 2020

Abstract
Background: Melanoma-associated antigen D1 (Maged1) is expressed in most adult tissues, predominantly in the brain, and has critical functions in the central nervous system in both developmental and adult stages. Loss of Maged1 in mice has been linked to depression, cognitive disorder, circadian rhythm, and drug addiction. However, the role of Maged1 in Parkinson’s disease (PD) remains unclear.Methods: Immunostaining was performed to investigate the expression of Maged1 in the samples from mice and human. To make the acute mice model of PD, C57BL/6 mice and Maged1 knockout mice were injected with 20 mg/kg 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) four times, every 2-hour intervals. SY5Y cells were treated by 200 μM 1-Methyl-4-phenylpyridinium iodide (MPP+). To examine motor balance and coordination, the rotarod test and pole test were used. Then we further investigated the role of Maged1 deficiency in DA neurons by high-performance liquid chromatography, immunohistochemistry, western blot, CCK8 assay, and gene transfection in vivo or in vitro.Results: Maged1 was expressed in DA neurons of samples from mice and human. And the expression of Maged1 was time-dependently upregulated by the treatment with MPTP or MPP+ in vivo or in vitro. Knockout of Maged1 in mice partly rescued the motor deficits and the reduced levels of striatal dopamine and its metabolites by MPTP treatment. Moreover, Maged1 deficiency protected primary DA neurons and differentiated ReNcell VM cells from MPP+ toxicity. Furthermore, along with the overexpression or downregulation of Maged1 in cultured SH-SY5Y cells, the reduced the cell viability by MPP+ treatment was relatively aggerated or attenuated. The effect of Maged1 deficiency may be attributed to the upregulated Akt signaling pathway and the downregulated mTOR signaling pathway, which further attenuated the MPTP or MPP+ -induced cell apoptosis and impairment of autophagy. Consistent with the above data, the degeneration of midbrain and striatum among 15-m Maged1 knockout mice was relatively mild compared to those in 15-m wild-type mice under physiological conditions.Conclusions: Maged1 deficiency-mediated apoptosis inhibition and autophagy enhancement may be a potential pro-survival mechanism during the progression of PD.
Identification of INHBA as a Potential Biomarker for Gastric Cancer by Comprehensive Analysis
Authors: LIU Guang-jie , HU Xiaojie , Jia Yuxuan , Huo Bingjie , Yang Lei , Liu Fang 
Published: October 2020

Abstract
BackgroundInhibin subunit beta A (INHBA) is a member of the TGF-beta (transforming growth factor-beta) superfamily proteins, which plays a fundamental role in various cancers. However, there is little systematical analysis on the exact role of INHBA in patients with gastric cancer (GC). Herein, we explored the exact role and the underlying mechanisms of INHBA regarding GC using multiple bioinformatic approaches. MethodsThe expression levels of INHBA in GC were analyzed in TIMER, GEPIA2, GEO, Oncomine and UALCAN databases. Protein and PCR test were performed to verify the expression states of INHBA in GC tissues. The correlation of INHBA and prognosis of GC was analyzed based on Kaplan Meier plotter database. Besides, the relationship between INHBA expression and immune infiltration levels and the type markers of immune cells in GC was explored in the TIMER database. What’s more, we studied INHBA mutations, promoter methylation, functional enrichment analysis in GC patients based on cBioportal, MEXPRESS, Metascape and LinkedOmics databases. Besides, we performed immunohistochemistry (IHC) and polymerase chain reaction (qRT-PCR ) verification in tissues from patients with gastric cancerResultsINHBA was elevated in GC and high expression level of INHBA in GC was significantly related to the unfavorable prognosis. Protein and PCR test verified the highly expression states of INHBA in GC tissues. Further analysis showed that INHBA was negatively correlated with B cell while positively correlated with the marker type of CD8+ T cells, macrophage, neutrophil and dendritic cell infiltration. High INHBA expression level had a poor prognosis in different enriched immune cells subgroups in GC. And there is week significant methylation level change between tumor and normal tissues. Moreover, INHBA mainly enriched cancer-related signaling pathways, including TGF-beta signaling pathway, ECM-receptor signaling pathway, PID ALK1/2 pathway, and AGE-RAGE signaling pathway. ConclusionsThe present study implies that INHBA may serve as a potential biomarkers for predicting prognosis in GC patients. High INHBA expression in GC may affect prognosis through immune infiltration.
Burnout and Stress Among Emergency Physicians in the Kingdom of Bahrain Using the Copenhagen Burnout Inventory (CBI) and Short Form-stress Overload Scale (SOS-S)
Authors: Abuzeyad Feras , Bashmi Luma , Das Priya , Ansari Abrar Al , Hsu Stephanie , Qasim Ghada Al , Mansoor Naser Mohamed Ali , Almusalam Abdulla 
Published: October 2020

Abstract
Background: Emergency physicians (EPs) are exposed to various stressors that lead to burnout, and these factors have never been examined in the emergency departments (EDs) of the Kingdom of Bahrain. The study identifies the prevalence rates and associated variables for burnout and stress among EPs in Bahrain’s EDs.Methods: A cross sectional study involving 134 EPs working in the ED for the three major tertiary hospitals completed the Copenhagen Burnout Inventory (CBI) and Stress Overload Scale-Short Form (SOS-S).Results: The survey had a response rate of 86.7% (n=116). The prevalence rate was 81.0% for personal burnout (M: 63.0, SD: 22.4; CI: 67.0% - 83.3%), 69.8% for work-related burnout (M: 60.3, SD: 21.6; CI: 60.6%- 78.0%), and 40.5% for patient-related burnout (M: 43.1, SD: 25.4; CI: 31.5% - 50.0%). Approximately 23.9% EPs were at high risk for illness. The CBI and SOS-S subscales had statistically significant correlations, illustrating that higher personal, work-related, and patient-related burnout was associated with higher personal vulnerability, event load, and stress overload. Being female, Bahraini, Chief Resident or Consultant, working >50 hours per week, taking at least one sick leave in the last year, and experiencing sleep disturbances and workplace violence were all contributing factors to higher burnout and stress levels. Conclusion: The prevalence of burnout and stress among the EPs in Bahrain is high and reflects a significant problem. Several demographic and occupational factors are closely related to burnout and stress, and need to be addressed to higher authorities in order to implement protective measures.
Rice Root-Associated Diazotrophic Community Succession is Driven by Growth Period Combined With Fertilization
Authors: Cui Zhongli , Luo Xue , Ye Xianfeng , Wang Wenhui , Chen Yang , Dong Chaonan , Li Zhoukun , Qiao Yan , Zhao Yuqiang , Huang Yan , Ran Wei , Huan Minghui , Fang Xiaodong , Han Jian , Cao Hui 
Published: October 2020

Abstract
BackgroundBiological nitrogen fixation (BNF) and nitrogenous fertilizers are two crucial ways for nitrogen input in the rice paddy system. Little is known about the effect of nitrogenous fertilizers on root-associated diazotrophs. Here, we investigated the succession of total and active diazotrophs in rhizosphere soil and rice roots under four fertilization treatments (control, NPK, NPK + pig manure, and NPK + rice straw) during three key growth periods (tillering, heading and mature).ResultsThe 15N isotope dilution experiment verified that root-associated diazotrophs could supply N for rice under low nitrogen nutrition by BNF (%Ndfa=11.51). Niche differentiation of diazotrophs existed at the rhizosphere soil-root interface. At both DNA and RNA levels, growth period had stronger effects on the community composition of endophytic diazotrophs than rhizosphere diazotrophs. The Chao1 and Shannon indices of total endophytic diazotrophs dramatically increased at the vegetative stage, and underwent relatively minor changes at the reproductive stage. Furthermore, the community structures of total endophytic diazotrophs were more stabilized in the reproductive stage than the vegetative stage. The number of OTUs shared by rhizosphere soil and roots increased during rice growth. Compared with CK, NPK reduced the relative abundances of Pelobacter and increased the relative abundances of Azoarcus. Pig manure not only improved soil nutrients (OM, TN, TP, AN, AP and NO3-N), but reduced the effect of chemical fertilizers on the community composition of natural rhizosphere diazotrophs. ConclusionsGrowth period demonstrated a stronger influence on the root-associated diazotrophs than fertilization practices. The community structures of total endophytic diazotrophs were more stabilized in the reproductive stage than the vegetative stage, and the alpha-diversity indices and complex of network structures of endophytic diazotrophs increased at the vegetative stage. Replacing chemical fertilization with pig manure not only increased soil nutrients, but regulated rhizosphere diazotrophic community structures. Understanding the combined effects of growth period and fertilization on root-associated diazotrophs presents the basis towards the sustainable crop production.
Insights into the Mechanism of Bovine Spermiogenesis Based on Comparative Transcriptomic Studies
Authors: Li Xin , Duan Chenying , Li Ruyi , Wang Dong 
Published: October 2020

Abstract
To reduce the reproductive loss caused by semen quality and provide theoretical guidance for the eradication of human male infertility, differential analysis of the bovine transcriptome among round spermatids, elongated spermatids, and epididymal sperm was carried out with the reference of the mouse transcriptome, and the homology trends of gene expression to the mouse were also analysed. First, to explore the physiological mechanism of spermiogenesis that profoundly affects semen quality, homological trends of differential genes were compared during spermiogenesis in dairy cattle and mice. Next, the Gene ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment, protein-protein interaction network (PPI network), and bioinformatics analysis uncovered the regulation network of acrosome formation during the transition from round to elongated spermatids. In addition, processes that regulate gene expression during spermiogenesis from elongated spermatid to epididymal sperm, such as ubiquitination, acetylation, deacetylation, glycosylation, and the functional gene ART3 may play an important role during spermiogenesis. Therefore, its localisation in the seminiferous tubule was investigated by immunofluorescent analysis, and its structure and function were also predicted. This study provides important data for revealing the mystery of life during spermiogenesis resulting from acrosome formation, histone replacement, and the fine regulation of gene expression.