bluuebunny/crossref_metadata_2025_split huggingface dataset:
Breast cancer metabolic subtypes analysis and risk prognostic models construction based on glycolysis metabolism genes
Authors: Liu Longbi , Zhen Wang , Cai Yufei , Liu Longxia , Zhang Yidan , LI Yuemeng , Liu Jintao 
Published: December 2024

Abstract

Glycolysis metabolism is an essential part of cancer research due to its role in cancer initiation and progression. However, its characteristics and prognostic value in breast cancer (BRCA) have not been systematically evaluated.We collected glycolysis metabolism gene expression profiles and clinical information of BRCA patients from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases. After excluding individuals lacking clinical information and the presence of genetic mutations, we performed consistent clustering of the remaining patients based on glycolysis metabolism gene expression profiles and selected stable clustering results to group patients. Differentially expressed genes (DEGs) and gene set enrichment analysis (GSEA) were compared between subgroups, while metabolic signature identification and decoding the tumor microenvironment were performed. In addition, we explored the survival status of patients among different subgroups and identified signature genes affecting survival by Least Absolute Shrinkage and Selection Operator (LASSO) regression. Finally, we selected signature genes to construct risk prognostic models by multivariate Cox regression.By consensus clustering, patients were distinguished into two stable subpopulations, GSEA and metabolic signature identification effectively defined two completely different subtypes of glycolysis metabolism: glycolysis hyperactive subtype and glycolysis hypoglycemia subtype. Among them, patients with the glycolysis hyperactive subtype had a poorer prognosis, with a significantly lower proportion of Macrophages M1 infiltration within the tumor microenvironment than others. Eight key genes, DEP domain containing 1(DEPDC1), Ras related GTP binding D(RRAGD),Phosphoglycerate kinase 1(PGK1),Secreted Frizzled-related Protein 2(STC2),Syndecan-1(SDC1), Lactate Dehydrogenase A(LDHA) ,Calpain 5(CAPN5),calcium channel, voltage-dependent, alpha 1H subunit(CACNA1H), were selected by multivariate Cox regression, which constructed a risk prognostic model.Our study revealed the heterogeneity of glycolysis metabolism in BRCA patients, defined two completely distinct subtypes of glycolysis metabolism, and finally established a novel glycolysis metabolism-related risk prognostic model. The study contributes to the early risk assessment and monitoring of individual prognosis and provides data to support individualized patient treatment.
Do Atypical Femoral Fractures Have Atypical Blood Tests? A Comparison of Atypical vs Typical Femoral Fractures
Authors: Brolchain Aongus O , Engelbrecht Zander , Steer Richard , Phillips Alfred , Lin Chen-i 
Published: December 2024

Abstract

Purpose: Atypical femoral fractures (AFFs) are a rare but important complication of anti-resorptive therapy (ART). The pathophysiology of these fractures remains poorly understood. AFFs are associated with low serum alkaline phosphatase (ALP) and other bone turnover markers, but the diagnosis is established on radiological grounds. This study investigated differences in serum biochemistry at time of hospitalisation AFFs and ‘typical’ femoral fractures (TFFs).
Methods: : Retrospective observational study across two centers in Queensland, Australia between 2012 – 2022. All femoral shaft fractures presenting across two hospitals between 2012 – 2022 were screened for inclusion. Two groups comprising AFFs and TFFs were identified for comparison. Blood results at the time of hospital admission were reviewed from the electronic medical record.
Results: 143 fractures were included, with 41 patients in the AFF group and 102 in the TFF group. Significant differences were observed between AFF and TFF groups in median serum ALP (56IU/L vs 83IU/L, p=<0.001), vitamin D level (86.8nmol/L vs 69.5nmol/L, p = 0.01), albumin (38g/L vs 36g/L, p = 0.015), globulin (27g/L vs 29g/, p = 0.02), creatinine (68 vs 77, p = 0.05) and B12 (360pg/ml vs 250pg/ml, p = 0.03). In the AFF group, 22.5% patients had a serum ALP <40IU/L compared with 3.9% in the TFF group. There was no significant difference observed in the remaining markers of liver function, electrolytes, blood group or coagulation profile.
Conclusion: Most datapoints were similar between groups. These data demonstrate that AFFs are associated with low serum ALP levels. Lower creatinine levels in the AFF group may reflect reduced muscle mass and increased frailty in these patients.
Association Between Estimated glucose disposal rate and Erectile Dysfunction in US Adults：a cross- sectional analysis of the NHANES 2001-2004
Authors: Xu+ Ningning , Wang+ Kexin , Xu Huanying , Zou Haoxi , Chen Yanfen , Wu Suzhen 
Published: December 2024

Abstract

Background
Erectile dysfunction (ED) is a sexual health issue affecting approximately 152 million men worldwide. Diabetes is a significant risk factor for ED, with insulin resistance playing a pivotal role in its pathogenesis. The Estimated Glucose Disposition Rate (eGDR), has been validated as a convenient and non-invasive indicator for assessing insulin resistance in diabetic patients. However, no studies have yet explored the relationship between eGDR and ED.
Objective
The purpose of this study was to determine the association between eGDR and ED in U.S. men from the National Health and Nutrition Examination Survey (NHANES).
Design:
The study analyzed data from 1,844 men aged 20 years and older in the 2001–2004 NHANES. Multivariate logistic regression with sampling weights assessed the association between eGDR and ED. Smoothed curve fitting were employed to explore the linearity of the eGDR-ED relationship. Subgroup analyses and interaction tests were performed to ensure the robustness of the result.
Result
We found a negative connection between eGDR and ED in men. Each unit increase in eGDR is associated with a 22% reduction in the risk of ED [odds ratio (OR) = 0,78 ,95% confidence interval (95% CI): 0.72–0.85, P < 0.0001].
Conclusion
Estimated glucose disposal rate was related to risk of Erectile Dysfunction in the United State adults, increase in eGDR is associated with a reduction in the risk of ED.
Methyl acrylate Functionalized Hyper-Crosslinked Polymers and Their Efficient Adsorptive Removal of Aromatic Small Molecule Compounds from Water
Authors: Huang Xiaobei , Wang Xiaolin , Zhu Shibao , Zhang Qingyuan , Wang Yutong , Fu Yuli , Xiao Zisheng , Xu Mancai , Zhong Shihua 
Published: December 2024

Abstract

Herein, methyl acrylate functionalized hyper-crosslinked polymers (HSDMs) were synthesized using the suspension polymerization method and they were employed to adsorb aromatic small molecule compounds. Low-crosslinked precursor polymers were prepared using styrene (St) and divinylbenzene (DVB) as crosslinking agents and different ratios of methyl acrylate (MA, 20, 15, 10, 5%) as functional monomers. Subsequently, the Friedel-Crafts reaction was conducted at 313 K to yield products characterized by hyper-crosslinked interpenetrating polymer networks abundant in rigid methylene bridges. The resulting polymers were promising for the adsorption of aromatic small molecule compounds, and the maximum capacities (qmax) for aniline, phenol and salicylic acid arrived at 103.79, 98.75 and 229.98 mg/g. The kinetic experiments demonstrated that adsorption aniline, phenol and SA reached the equilibrium within 60, 60 and 150 min, and the kinetic results were accurately described by the pseudo-second-order (PSO) rate model, exhibiting kinetic rates of 8.34×10-4, 1.01×10-3, and 5.00×10-4 g/(mg·min), respectively. Dynamic adsorption aniline capacity of 50.06 mg/g on HSDM-5 and desorption efficiency of 90.74 %. The adsorption mechanism discovered that pore-filling, hydrogen bonding, hydrophobic interaction and π-π stacking were important for the adsorption.
Working Conditions and Environment of Maternal and Neonatal Healthcare Providers in the Context of Armed Conflicts and Humanitarian Crises: The Case of Eastern DR Congo
Authors: Lembebu Corneille , Ngaboyeka Gaylord , Bisimwa Ghislain , Ndokabilya Eustache , Makali Samuel , Zalinga Christian Chiribagula , Bugugu Bony , Byamungu Christian Mugisho , Murhima’alika Christine Chimanuka , Bigirinama Rosine N. , Dramaix Michèle , Hermans Michel P , Mwene-Batu Pacifique 
Published: December 2024

Abstract

Introduction
Healthcare worker (HCW) play a crucial role in reducing maternal and infant mortality. However, in contexts of endemic armed conflicts, such as in the Democratic Republic of Congo (DRC), these professionals must operate under extreme working conditions, which can affect the quality of care. This study aims to assess the working environment and challenges faced by maternal and neonatal HCW in the DRC.
Methodology
We conducted a cross-sectional study in the North and South Kivu provinces in Eastern DRC. The study primarily targeted healthcare facilities in eight health zones. An evaluation aligned with the structure/process dimensions (profile and perception of the work environment of HCW) according to Donabedian was conducted. A descriptive and comparative analysis of the HZs (using the Chi-square test and T test) was performed. Informed consent was obtained for participation in the study.
Results
A total of 200 HCW were enrolled in the study, 71% of whom were women, with an average age of 37.5 years. Nurses were the majority, followed by midwives, while general practitioners represented 10.67%. On average, HCW worked approximately 67 hours per week. A third of HCW did not feel well-prepared and are dissatisfied with their work environment, reporting gaps in discussions about interpersonal skills, promotion opportunities, and professional development. Disparities in treatment were observed, with notable inequalities in training, time off, work hours, function, and promotions, often exacerbated by gender. Less than 30% received bonuses, and 90% did not receive non-monetary incentives. Verbal threats and assaults, humiliations, and physical assaults from colleagues and patients were common, with a higher incidence in North Kivu. Finally, nearly a quarter of HCW felt exposed to insecurity in and around their facilities, a perception more pronounced in North Kivu than in South Kivu.
Conclusion
This study shows that HCW in the DRC, particularly in conflict zones, work under extremely difficult conditions, characterized by intensive hours, lack of training, professional inequalities, and high insecurity. These challenges may compromise quality of care and the well-being of providers, underscoring the urgency of strengthening their protection and support to maintain the resilience of the healthcare system.
Differential effects of NMDAR antagonists on working memory and gamma oscillations, and the mediating role of the GluN2D subunit
Authors: Hill Rachel , Vinnakota Chitra , Hudson Matthew , Ikeda Kazutaka , Ide Soichiro , Mishina Masayoshi , Sundram Suresh , Jones Nigel 
Published: December 2024

Abstract

Working memory relies on synchronised network oscillations involving complex interplay between pyramidal cells and GABAergic interneurons. NMDA receptor (NMDAR) antagonists influence both network oscillations and working memory, but the relationship between these two consequences has not been elucidated. This study aimed to determine the effect of NMDAR antagonists on network oscillations during a working memory task in mice, and the contribution of the GluN2D receptor subunit.
After training wildtype (WT) and GluN2D-knockout (KO) mice on the Trial-Unique-Non-match to Location (TUNL) touchscreen task of working memory, recording electrodes were implanted into the prefrontal cortex (PFC) and hippocampus. Mice were challenged with either (S)-ketamine (30mg/kg), (R)-ketamine (30mg/kg), phencyclidine (PCP, 1mg/kg), MK-801 (0.3mg/kg) or saline prior to TUNL testing while simultaneous local field potential recordings were acquired.
PCP disrupted working memory accuracy in WT (p=0.001) but not GluN2D-KO mice (p=0.79). MK-801 (p<0.0001), (S)-ketamine (p<0.0001) and (R)-ketamine (p=0.007) disrupted working memory accuracy in both genotypes. PCP increased baseline gamma (30-80Hz) power in the hippocampus in WT (p=0.0015) but not GluN2D-KO mice (p=0.92). All drugs increased baseline gamma power in the PFC in both genotypes (p<0.05). Low gamma was induced during the maintenance phase of the TUNL task and increased when mice correctly completed the task (p=0.024). MK-801 disrupted task-induced low gamma in both genotypes (p=0.04).
In summary, PCP action involves the GluN2D subunit of the NMDA receptor in the hippocampus to alter baseline gamma power and working memory. Task-induced low gamma activity during maintenance aligns with task performance, and is disrupted specifically by MK-801.
Risk Factors of Decisional Conflict in People Living with Chronic Pain: a pan-Canadian survey.
Authors: Naye Florian , Tousignant-Laflamme Yannick , Sasseville Maxime , Cachinho Chloé , Gérard Thomas , Toupin-April Karine , Dubois Olivia , Paquette Jean-Sébastien , LeBlanc Annie , Gaboury Isabelle , Poitras Marie-Eve , Li Linda C. , Hoens Alison , Poirier Marie-Dominique , Légaré France , Décary Simon 
Published: December 2024

Abstract

Making decisions about chronic pain care is often challenging due to uncertainties, leading to decisional conflict when individuals do not receive the support and information they need. Shared decision-making interventions can help meet these needs; however, their effectiveness is inconsistent in the context of chronic pain. This study aimed to identify the decisional needs influencing decisional conflict among adults with chronic pain in Canada, to guide the development of more comprehensive interventions. In this pan-Canadian online survey, we measured decisional conflict related to the most difficult decision using the Decisional Conflict Scale (≥ 37.5 indicating clinically significant conflict) and assessed decisional needs based on the Ottawa Decision Support Framework. Of the 1,649 participants, 1,373 reported a Decisional Conflict Scale score. The mean age was 52 (SD = 16.4), with half of respondents being men (49.5%) and pain duration ranging from 3 months to 59 years. One-third (33.7%) experienced clinically significant decisional conflict. Seventeen risk factors were identified, including difficulty understanding healthcare information (OR = 2.43) and lack of prior knowledge of available options (OR = 2.03), while role congruence in decision-making was associated with reduced conflict (OR = 0.57). Future SDM interventions could be enhanced by targeting multiple risk factors of decisional conflict.
Digital chest drainage versus traditional chest drainage in children after pulmonary resection: A systematic review and meta-analysis
Authors: Ahmed Rakesh , Durr Anna , Healy David 
Published: December 2024

Abstract

Background
Pulmonary resection is commonly performed as curative treatment for congenital thoracic malformations. There is a plethora of high-quality evidence showing improved outcomes with digital chest drainage systems compared to traditional water-seal systems in adults. By contrast, there is a relative paucity of research with children and therefore much uncertainty in post-operative chest drain management in paediatrics.
Methods
A systematic review and meta-analysis was conducted to assess the effect of digital chest drainage systems in paediatric patients after pulmonary resection. Data sources included PubMed, Cochrane Central Register of Controlled Trials, EMBASE, and SCOPUS, with information from 1 January 2007 to 20 July 2024. The qualitative analysis included three observational studies. The quantitative analysis included 74 patients across two observational studies that compared digital and traditional chest drainage systems. A fixed effects model was used to produce a pooled estimate for a meta-analysis of means if the studies were homogenous, otherwise a random effects model was used.
Results
The meta-analysis showed a statistically significant reduction in hospital length of stay (SMD − 0.63 days, [95% CI: -1.1;-0.16, p = < 0.01]) and number of chest x-rays performed (SMD − 1.2 x-rays, [95% CI: -1.7;-0.7, p = < 0.0001]) in the digital group compared to the traditional group. Although a reduction in chest tube duration was seen in the digital group, this was not statistically significant (SMD − 2.2 days, [95% CI: -14.5;10.07, p = 0.2622]). There were no significant differences in development of pulmonary complications between the digital and traditional drainage groups (p = 0.8392).
Conclusion
The use of digital chest drainage systems demonstrated a shortened hospital length of stay and quantity of chest x-rays performed in paediatric patients following pulmonary resection. The overall certainty of these findings is limited by the low quality of the available evidence.
Investigation of the Effects of Galectin-1 on Enzalutamide Sensitivity and Cellular Functions in Prostate Cancer Cells and Its Clinical Diagnostic Value
Authors: Lee Ya-Che , Yang Kai-Wu , Lai Ya-Yan , Tung Chun-Liang , Shen Cheng-Huang , Chen Jui-Chieh 
Published: December 2024

Abstract

Prostate cancer (PCa) is the most common malignancy in men worldwide, with an increasing incidence rate, and many patients are diagnosed at an advanced stage with metastasis, where bone metastasis is the most frequent. Enzalutamide (ENZ) is the first-line treatment for castration-resistant prostate cancer (CRPC) and has shown efficacy in prolonging survival; however, some patients develop drug resistance. Additionally, benign prostatic hyperplasia (BPH) is a common prostate condition in aging men, with symptoms similar to PCa, highlighting the need for improved diagnostic and therapeutic approaches. In the present study, we integrated public databases, RNA sequencing (RNA-Seq), and bioinformatics tools, utilizing CRISPR-Cas9 technology to knock out galectin-1 (LGALS1) in PCa cells to comprehensively investigate its impact on ENZ sensitivity, cellular functions, and the underlying mechanisms. Clinical tissue samples were also analyzed to assess the clinical significance of LGALS family members. Our findings indicated that ENZ sensitivity depends on AR expression, and LGALS1 knockout in AR-expressing cells enhances ENZ sensitivity. RNA-Seq revealed that LGALS1 knockout suppresses energy metabolism and disrupts oxidative stress balance. Additionally, LGALS1 knockout in high-expression cells reduced proliferation, altered the cell cycle, and decreased migration and adhesion. Clinically, both AR and LGALS1 were overexpressed in bone-metastatic PCa, suggesting their potential as therapeutic targets and biomarkers.
Epidemiological features of hypertension in a high-altitude population in Tibet, China: a cross-sectional study
Authors: Yu Yue , Peng Jieru , Zhaxi Zongji , Jimei Quzhen , Shi Heng , Bai Guoxia , Yang Chunxia 
Published: December 2024

Abstract

Objectives To explore the present epidemiological status of hypertension in the high-altitude regions of Tibet, China, and offer insights for the prevention and control strategies of hypertension in this population.
Design A cross-sectional study was designed to be conducted in seven cities (localities) in the Tibet Autonomous Region (TAR).
Participants and settingThe survey employed a multi-stage stratified cluster sampling method and enrolled 8,992 residents aged ≥35 years from seven cities in Tibet.
Main outcome measures Present status of the epidemiology of hypertension.
Results The prevalence of hypertension in Tibet was 46.5 % and the weighted prevalence was 46.7 %. Hypertension at stage 2 and above accounted for 49.8% of all cases. Of the hypertensive subjects included in this study, 45.2 % had been diagnosed with hypertension before the study, 30.8 % were taking antihypertensive medication, and only 3.0 % had effective blood pressure control. Aging, urban population, alcohol consumption, overweight, obesity, diabetes mellitus and central obesity were risk factors for hypertension (all p < 0.05 ). Moreover, 94.2% of the individuals using antihypertensive drugs received treatment with only one medication, and calcium channel blockers were the most frequently prescribed antihypertensive drug for single-drug therapy.Women were more likely to use calcium channel blockers compared to men, while beta-blockers were less commonly used by women in comparison to men (p<0.05), nifedipine was the most commonly used calcium channel blocker drug in patients with hypertension.
Conclusion Hypertension is a key public health problem in Tibet, associated with multiple factors and with low rates of awareness, treatment and control. The government needs to strengthen basic medical care and health promotion for high-altitude populations in Tibet.
Optimising Caesarean section use in Nepal through hospital generated and population-based data on Caesarean section rates to inform policy and practice
Authors: Iang Maureen Dar , Rath Alison Dembo , Devkota Madhu Dixit , Sapkota Vishnu , Mahendra Shanti 
Published: December 2024

Abstract

Background:
The dual problem of lack of access to life-saving caesarean section (C-section) and the negative effects of over-intervention when it is not medically required, is well established. The health burden plus additional direct and indirect costs for households and the health system, create a ‘triple burden.’ In Nepal, whilst C-section rates have rapidly escalated, our data suggests an opportunity to avert a C-section epidemic and reduce unmet need for C-section among women with very low income.
Methods
We analysed C-section rates from 29 public and private hospitals across Nepal using the Robson Ten Group Classification System and assessed how each group compared with the Robson reference groups. Using the WHO C-Model we estimated the gap between observed and predicted facility C-section rates, adjusted for the obstetric case-mix at each facility. We generated estimates for four categories of hospitals: public with Aama (the government demand-side financing programme); public without Aama; private with Aama; private without Aama.
Results
We found that Robson groups 1 and 3 (nulliparous or multiparous women with single cephalic pregnancy, ≥ 37-week gestation, spontaneous onset of labour), together account for 66.5% of C-sections of total institutional deliveries. All four hospital categories had higher C-section rates than predicted rates. The percentage points difference between observed and predicted rates was greater for private hospitals (with Aama 18.6 percentage points and non-Aama 19.7 percentage points), than government hospitals (with Aama 10.8 percentage points and non-Aama 13.9 percentage points). The biggest gap was for private hospitals (non-Aama with observed C-section rate at 42% compared with 23% predicted.
Conclusions
Our hospital-based study data provides evidence that C-section rates are too high relative to need and are not just a function of late arrivals or complicated births, as is often claimed by facilities. C-section rates can be controlled by introducing measures in both public and private sectors. Policy needs to continue to focus on increasing uptake of the life-saving intervention among populations where C-section rates fall below optimal levels, while also ensuring that they are avoided if not indicated medically. Resources for maternal and newborn health need to be reallocated to achieve uptake of medically required C-sections equitably across population groups and steer Nepal towards optimal rates.
Clinical trial number: Not applicable.