bluuebunny/crossref_metadata_2025_split huggingface dataset:
SWATH-MS analysis of cerebrospinal fluid to generate a robust battery of biomarkers for Alzheimer’s disease
Authors: Park Sun Ah , Jung Jin Myung , Park Jun Sung , Lee Jeong Ho , Park Bumhee , Kim Hyung Jun , Park Jeong-Ho , Chae Won Seok , Jeong Jee Hyang , Choi Seong Hye , Baek Je-Hyun 
Published: May 2020

AbstractCerebrospinal fluid (CSF) Aβ42 and tau protein levels are established diagnostic biomarkers of Alzheimer’s disease (AD). However, their inadequacy to represent clinical efficacy in drug trials indicates the need for new biomarkers. Sequential window acquisition of all theoretical fragment ion spectra (SWATH)-based mass spectrometry (MS) is an advanced proteomic tool for large-scale, high-quality quantification. In this study, SWATH-MS showed that VGF, chromogranin-A, secretogranin-1, and opioid-binding protein/cell adhesion molecule were significantly decreased in 42 AD patients compared to 39 controls, whereas 14-3-3ζ was increased (FDR < 0.05). In addition, 16 other proteins showed substantial changes (FDR < 0.2). The expressions of the top 21 analytes were closely interconnected, but were poorly correlated with CSF Aβ42, tTau, and pTau181 levels. Logistic regression analysis and data mining were used to establish the best algorithm for AD, which created novel biomarker panels with high diagnostic value (AUC = 0.889 and 0.924) and a strong correlation with clinical severity (all p < 0.001). Targeted proteomics was used to validate their usefulness in a different cohort (n = 36) that included patients with other brain disorders (all p < 0.05). This study provides a list of proteins (and combinations thereof) that could serve as new AD biomarkers.
Multiplex bioimaging of single-cell spatial profiles for precision cancer diagnostics and therapeutics
Authors: Allam Mayar , Cai Shuangyi , Coskun Ahmet F. 
Published: May 2020

AbstractCancers exhibit functional and structural diversity in distinct patients. In this mass, normal and malignant cells create tumor microenvironment that is heterogeneous among patients. A residue from primary tumors leaks into the bloodstream as cell clusters and single cells, providing clues about disease progression and therapeutic response. The complexity of these hierarchical microenvironments needs to be elucidated. Although tumors comprise ample cell types, the standard clinical technique is still the histology that is limited to a single marker. Multiplexed imaging technologies open new directions in pathology. Spatially resolved proteomic, genomic, and metabolic profiles of human cancers are now possible at the single-cell level. This perspective discusses spatial bioimaging methods to decipher the cascade of microenvironments in solid and liquid biopsies. A unique synthesis of top-down and bottom-up analysis methods is presented. Spatial multi-omics profiles can be tailored to precision oncology through artificial intelligence. Data-driven patient profiling enables personalized medicine and beyond.
Whole exome sequencing of a family revealed a novel variant in the CHM gene, c.22delG p.(Glu8Serfs*4), which co-segregated with choroideremia
Authors: Dan Handong , Li Tuo , Lei Xinlan , Huang Xin , Xing Yiqiao , Shen Yin 
Published: May 2020

Abstract
Choroideremia is a complex form of blindness-causing retinal degeneration. The aim of the present study was to investigate the pathogenic variant and molecular etiology associated with choroideremia in a Chinese family. All available family members underwent detailed ophthalmological examinations. Whole exome sequencing, bioinformatics analysis, Sanger sequencing, and co-segregation analysis of family members were used to validate sequencing data and confirm the presence of the disease-causing gene variant. The proband was diagnosed with choroideremia on the basis of clinical manifestations. Whole exome sequencing showed that the proband had a hemizygous variant in the CHM gene, c.22delG p. (Glu8Serfs*4), which was confirmed by Sanger sequencing and found to co-segregate with choroideremia. The variant was classified as likely pathogenic and has not previously been described. These results expand the spectrum of variants in the CHM gene, thus potentially enriching the understanding of the molecular basis of choroideremia. Moreover, they may provide insight for future choroideremia diagnosis and gene therapy.
Application of TOPSIS Method Based on Entropy Weight -Delphi in the Evaluation of Teachers’ “double-qualified” Ability
Authors: Wang Weixia , Jun He 
Published: May 2020

In order to improve the rationality and fairness of Teachers’ “Double-qualified” Ability, the article establishes an evaluation model based on 14 evaluation indexes of teachers’ “double-qualified” ability. it adopts Delphi - entropy weight method to weight the evaluation index, and then combines TOPSIS method to evaluate the evaluation object. In the evaluation of TOPSIS method, the traditional TOPSIS weight method was improved, and the entropy weight-delphi method was used to determine the index weight, which was a combination of subjective and objective, making the evaluation system more objective, scientific and reasonable.It not only avoids the subjectivity of decision makers and limitations, but also eliminates the phenomenon of indexes in common impact assessment results and finally applies it to a university teacher “Double division and triple energy” evaluation system, to provide theoretical basis and feasibility analysis for the “double type” teachers team construction.
Chinese library classification number: O224 Document identification code: A
L’innovation thérapeutique, à quel prix ?
Authors: Fischer Alain , Dewatripont Mathias , Goldman Michel 
Published: May 2020

Comment préserver l’accessibilité aux nouveaux médicaments pour tous les patients qui en ont besoin ? La question se pose à la lumière des prix exorbitants auxquels les thérapies géniques, mais aussi les biothérapies et de nouvelles molécules chimiques, sont commercialisées. L’analyse montre qu’à court/moyen terme, l’arrivée sur le marché d’un nombre croissant de nouveaux traitements à haute valeur ajoutée mais à des prix très élevés deviendra insoutenable pour les organismes payeurs qui sous-tendent notre système de santé. Les arguments de rentabilité invoqués par les sociétés pharmaceutiques pour justifier les prix proposés sont à tout le moins contestables, d’autant plus qu’ils ne sont pas documentés de façon transparente et que cette industrie jouit déjà de marges bénéficiaires considérables, en comparaison de celles d’autres secteurs du monde économique. Quant aux promesses de guérison définitive assurée par certains traitements, il faudra encore attendre quelques années pour en vérifier le fondement. À l’aube d’une réforme qui apparaît incontournable, nous formulons plusieurs propositions pour faire avancer le débat, en particulier : (1) mettre en œuvre la résolution avancée par plusieurs états membres de l’Organisation mondiale de la santé (OMS) pour assurer la transparence des coûts de développement des nouveaux médicaments ; (2) imposer une clause de prix raisonnable dans les accords qui régissent le transfert de technologies entre les institutions académiques soutenues par des fonds publics et les entreprises privées ; (3) instituer une instance européenne commune de négociation des prix des médicaments adaptée aux conditions socioéconomiques de chaque pays ; (4) conditionner le remboursement des traitements innovants à leur commercialisation par des entreprises - ou des filiales de celles-ci - répondant à des normes certifiant leur responsabilité sociétale et (5) développer en Europe des partenariats public-privé à but non lucratif, pour la création de quelques unités de production de biothérapies et thérapies géniques. L’objectif général de ces mesures serait de fixer un cadre qui permette de définir des prix qui garantissent un juste retour de l’investissement public vers le système de santé tout en assurant pour les entreprises un profit d’un niveau conforme au marché et une incitation suffisante à investir dans les domaines prioritaires pour la santé publique.
BLACK HIP-A CASE OF OCHRONOTIC ARTHROPATHY
Authors: Mathias Lawrence J. , Mathias Michelle , Shetty Padma , Rai Ravindranath 
Published: May 2020

Abstractalkaptonuria an autosomal recessive disorder in which a deficiency of homogentessic acid oxidase results in the accumulation of homogentessic acid which is deposited in connective tissues notably in the intervertebal disc,articular cartilage and tendons leading to their early degeneration.Here we describe a case of ochronotic arthropathy
MICROFILARIA IN PERICARDIAL EFFUSION COEXISTING WITH SPINDLE CELL THYMOMA-A RARE CASE REPORT
Authors: Permi Harish S , N. Samaga B , K. Subramanyam , K. Jayaprakash Shetty , S. Teerthanath , Baikunje Vidya , Ninan Kurian 
Published: May 2020

AbstractLymphatic filariasis is common in tropical countries and is endemic in South India. Filariasis presenting with pericardial effusion is rare, coexisting with spindle cell thymoma is still very rare. We report a case of a 53-year-old male who was admitted to cardiac intensive care unit with severe breathless since two days. Cardiac Echocardiogram showed massive pericardial effusion with cardiac tamponade and large anterior mediastinal mass. Pericardial fluid aspiration drained 1.5 litres of hemorrhagic fluid. Cytology examination revealed microfilaria of Wutchereria bancrofti. FNAC and trucut biopsy from anterior mediastinal mass showed features of spindle cell thymoma. He was treated with diethyl carbamazine thrice daily for one month. He refused for excision of thymoma. This case report is a rare coexistence of microfilaria in pericardial effusion with spindle cell thymoma. We stress the need to consider filariasis as differential diagnosis in the evaluation of hemorrhagic pericardial effusion especially in endemic areas.
ELLIS VAN CREVELD SYNDROME WITH PARTIAL ATRIOVENTRICULAR CANAL DEFECT, PERSISTENT LEFT SUPERIOR VENA CAVA, DUAL CORONARY SINUS OSTIAE - A CASE REPORT
Authors: Gopalakrishnan Mundayat , Kamath Manjunath , Patla Krishna Prasad , Subramanyam Kodangala 
Published: May 2020

AbstractEllis van Creveld syndrome (EVCS) is a congenital defect with skeletal and cardiac anomalies. We have operated on a 30 year old female with EVCS having single atrium, severe mitral and tricuspid regurgitation, bilateral superior venacavae, double coronary sinus ostiae .Anterior Mitral cleft repair, a tricuspid annuloplasty, rerouting of left superior venacava and septal patching were done. She made a smooth recovery
ORAL AND CRANIOFACIAL CHARACTERISTICS OF UNTREATED ADULT UNILATERAL CLEFT LIP AND PALATE INDIVIDUALS
Authors: Ravi M. S. 
Published: May 2020

Abstract
Introduction: Cleft lip and palate anomaly being the common congenital defect having significant effects on the facial morphology, function and growth and development of the individual, requires a detailed study of the Dental and craniofacial characteristics.
Design and setting: The study is conducted in a hospital set up and the subjects were selected at random as per the inclusion criterion. Objectiveof study was to evaluate the craniofacial and Dental morphology in untreated unilateral cleft lip and palate (UCLP) individuals.
Materials and Methods: A total of 64 individuals in the age group of 15-28 yrs were selected and grouped in to two groups; Cleft group consisting of 13 males and 19 females having untreated UCLP and another group of 16 male and 16female non cleft individuals. Study cast, lateral cephalogram and frontal cephalogram analysis were carried out .The obtained data were analyzed using ANOVA to compare the values between the groups.
Result: Significant differences in cranio- facial characteristics were noted between the cleft and non cleft individuals. These differences were more pronounced in sagittal plane and less in transverse plane.
Conclusion: Significant differences exist in the cranio- facial morphology between the untreated UCLP individuals and non cleft individuals.
ANCIENT SCHWANNOMA OF THE CHEST WALL DIAGNOSED BY FINE NEEDLE ASPIRATION CYTOLOGY- A RARE CASE REPORT
Authors: Bhat Shubha P. , Permi Harish S. , Shetty Rohan , Shenoy Jayaram , Prasad Kishan , Hegde Panna 
Published: May 2020

AbstractSchwannomas are benign peripheral nerve sheath tumors. Ancient schwannoma is an uncommon variant of schwannoma, which is usually located in the retroperitoneum. Those located in the chest wall are extremely rare. We report a case of ancient schwannoma in a 34 yr old male who presented with left sided chest pain, found to have a mass lesion in the chest wall radiologically. CT guided fine needle aspiration cytology showed features of ancient schwannoma which was confirmed on histopathology. Cytological findings in correlation with long standing nature and well defined borders on radiological examination of the lesion will aid in the preoperative diagnosis and proper management.
INTEROCCLUSAL RECORDS IN PROSTHODONTIC REHABILITATIONS - MATERIALS AND TECHNIQUES - A LITERATURE REVIEW
Authors: D. Krishna Prasad , Prasad B. Rajendra , D. Anupama Prasad , Mehra Divya 
Published: May 2020

AbstractTo achieve a successful prosthesis it is important to achieve harmony between the maxillomandibular relationship and anatomy of patient. This relationship is not simple opening or closing, but a complex relationship which exists in 3 dimensions. Variations may occur in any direction – vertical, anteroposterior, or mediolateral. Thus, it is essential to record this relationship with the least possible error to obtain a successful prosthesis. However when relating the maxillary and mandibular dental casts, the ultimate accuracy depends on accuracy and dimensional stability of the material and the technique used to record the maxillomandibular relationship.
A STUDY TO ASSESS THE PREVALENCE AND ATTITUDE OF USE OF TOBACCO PRODUCTS AMONG ADOLESCENT BOYS
Authors: Fernandes Sarita , S. Suchithra B. 
Published: May 2020

AbstractA simple descriptive design was adopted for the study to assess the attitude among the adolescent regarding tobacco consumption. The sample consisted of 100 adolascents boys, Convenient sampling method was used to select the samples. The data collection for the study was done by using demographic performa, questionnaire and rating scales. The analysis of the data was based according to the objectives and hypothesis. The results showed that- Majority of the consumers 57 (57%) were between the age group of 16-19 years of age , 39 (39%) were have secondary education, Attitude of adolescent boys towards tobacco consumption is negative (32%). There was no association between attitude and demographic variable like age, education and economic status etc (p<0.05).Smoking of cigarettes , particularly of “beedies” (which consist of about 0.2 to 0.3g of tobacco rolled up in the leaf of another plant (tambourine) has been widespread for many decades among men in India. There have, however, been no substantial studies on the prevalence of tobacco use among youth in India. The tobacco prevalence among girls is alarming. The results of survey show the need to increase awareness about health hazards of tobacco use among students.
Time between Diagnosis and Treatment of Hypopharynx and Larynx Cancer: Are Longer Delays Associated with Higher Discrepancy between Clinical and Pathological Staging?
Authors: Santos Mariline , Monteiro Eurico 
Published: May 2020

Abstract
Introduction At the time of diagnosis, treatment strategies for cancer are largely based upon clinical staging. However, discrepancy between clinical and pathological staging has been reported.
Objective To assess the rate of staging discrepancy in Laryngeal and Hypopharyngeal Squamous Cell Carcinoma (LHSCC), the potential influence of higher interval of time from diagnosis to primary surgical treatment, and whether this has any impact on survival outcomes.
Methods Retrospective study of patients with LHSCC proposed for primary surgical treatment.
Results The study population included 125 Caucasian patients with LHSCC. The level of agreement between clinical and pathological tumor staging was moderate (Cohen’s Kappa: 0.400; p < 0.001) and similar result was found for node staging (Cohen’ Kappa: 0.520; p < 0.001). The mean time between diagnosis and surgical treatment was 26.66 days and no statistically significant influence was found with staging discrepancy. The sample presented a 5-year Overall Survival (OS) of 58.2% and a Disease-specific survival (DSS) of 72.6%. No statistically significant impact of staging discrepancy on survival was found.
Conclusion For advanced LHSCC, based on the findings of physical examination, endoscopy and imaging, is possible to achieve a moderate accuracy between clinical and pathological staging which allows a reliable counselling and treatment planning. Interval of time under 3–4 weeks between diagnosis and surgical treatment does not influence the rate of discrepancy. However, almost 30% of staging discrepancy is expected due to false negatives of imaging and limitations of physical exams.
Management of Acute Variceal Bleed by Esophageal Variceal Ligation in an Unconventional Position—An Urban-Center Insight
Authors: Jalihal Umesh , Shivalingaiah Prithvipriyadarshini , Bale Abhijith 
Published: May 2020

Abstract
Background Acute variceal bleed is a fatal complication in patients with liver cirrhosis, and it is important to achieve hemostasis at the earliest. Endoscopic variceal ligation (EVL) is a preferred modality in controlling variceal bleed. The blood pool in esophagus and oozing of blood from varices cause hindrance for variceal ligation leading to failure of therapy. In sitting position, it is hoped that visualization of esophagus may be better. With this background, EVL of patients in sitting position was done and results were analyzed.
Methods Cirrhotics presenting with hematemesis and/or melena were included in the study. Clinical and endoscopic parameters were assessed. Among them, patients having hypotension and hepatic encephalopathy were excluded. Initial endoscopic assessment was done in left lateral position. In case of obscured visualization of esophagus due to flooding, if time taken to achieve hemostasis > 5 minutes, position was changed to sitting from left lateral decubitus. Patients with isolated gastric variceal and ulcer bleed were further excluded.
Results Among 78 acute bleed patients selected, 41fulfilled the inclusion criteria for the study. During endoscopy, in 20 patients, EVL was done in left lateral decubitus position with head end elevation. Twenty-one patients were shifted from conventional left lateral position to sitting posture. Study revealed that patients undergoing EVL in sitting position had better visibility aiding the procedure and no aspiration during the procedure. There was no difference in the left lateral with head raised or without it. Duration of EVL was comparable to that of nonbleeders and additional sclerotherapy was not required in any case in both the groups. Following the procedure, patients were observed for 5 days. There was no failure of therapy which was analyzed according to Baveno VI consensus. However, there was delayed complication in one case due to sepsis and metabolic acidosis causing death.
Conclusion This study suggests, in acute variceal bleed cases, EVL in sitting position aids in better visibility and helps in achieving hemostasis early with least complications.
Neurosurgical Emergencies at a Tertiary Referral Center in a Sub-Saharan African Country
Authors: Alihonou Thierry , Quenum Kisito , Padonou Adébola , Amossou François , Dossou Francis 
Published: May 2020

Abstract

Background The practice of neurosurgery in a teaching hospital requires modern diagnostic tools and a rigorous organization of care.

Objectives To present and discuss the management of neurosurgical emergencies in a teaching hospital in poor and low-income country.

Patients and Methods This is a retrospective and descriptive study from April 2015 to March 2017 and includes traumatic and nontraumatic neurosurgical emergencies. Epidemiological, diagnostic, operative, and outcome data were evaluated.

Results During the study period, 397 cases of neurosurgery were admitted. One hundred seventy-five of them were emergencies (43%), including 168 (96%) of traumatic origin. The average age was 32.5 years (1–80 years) with a male predominance: 149 men for 26 women, the sex ratio was 6.68. The cause of the neurotraumatological emergency was mostly road accidents with 143 cases (85.1%). The trauma was brain injury in 155 patients (92.3%) and spine injury in 13 patients (7.7%). In 64.3% of cases, diagnostic imaging was done beyond 48 hours. Surgery time was more than 48 hours when it was performed (21 cases). Outcome was good for 19 patients. Overall and postoperative mortality were, respectively, 34.5% (58 cases) and 9.5% (2 cases).

Conclusion Neurosurgical emergencies care at the Departmental Teaching Hospital of Ouémé–Plateau has become a common activity with encouraging operating results despite difficult practice conditions.
Implant Alignment and Patient Factors Affecting the Short-Term Patient-Reported Clinical Outcomes after Oxford Unicompartmental Knee Arthroplasty
Authors: Yamagami Ryota , Inui HIroshi , Taketomi Shuji , Kono Kenichi , Kawaguchi Kohei , Takagi Kentaro , Kage Tomofumi , Sameshima Shin , Tanaka Sakae 
Published: May 2020

AbstractThis study aims at clarifying implant alignment and other patient factors' influence on clinical outcomes, particularly on patient-reported outcomes (PRO), following Oxford unicompartmental knee arthroplasty (OUKA). A total of 142 patients after OUKA were divided into two groups according to the validated Japanese version of the knee injury and osteoarthritis outcome score (KOOS) for each subscale of pain, symptoms, and activities of daily living (ADL) at postoperative year 1 and 2: group 1 had ≥80 scores and group 2 had <80 scores. Postoperative clinical and radiographical findings were then compared among groups in each subscale. Using postoperative year 1 and 2 data, a multivariable logistic regression analysis was conducted to clarify factors for clinical outcomes' improvement. In the analysis of KOOS subscale of symptoms at postoperative year 1, gender distribution, preoperative body mass index, and postoperative maximum knee flexion angles differed significantly among groups. In the analysis for KOOS subscale of ADL, significant differences were observed in postoperative tibial component varus angles. Multivariable logistic regression analysis revealed that all of these parameters significantly related to ≥80 KOOS scores of each subscale at postoperative year 1. Additionally, at postoperative year 2, larger postoperative knee flexion angles and tibial component varus angles related to ≥80 KOOS subscale of symptoms and ADL were observed. In conclusion, tibial component's larger varus alignment was associated with the better PRO at both 1 and 2 years after OUKA. Importantly, larger postoperative knee flexion angle helped achieve good short-term PRO after OUKA.