bluuebunny/crossref_metadata_2025_split huggingface dataset:
The usefulness of re-attachability of anti-adhesive cross-linked gelatin film and the required physical and biological properties
Authors: Horii Tsunehito , Tsujimoto Hiroyuki , Kageyama Susumu , Yoshida Tetsuya , Kobayashi Kenichi , Takamori Hideki , Minato Hiroshi , Ueda Jo , Hagiwara Akeo , Ichikawa Hiroshi , Kawauchi Akihiro 
Published: November 2020

BACKGROUND: To overcome the unfavorable issues associated with conventional anti-adhesive HA/CMC film, we developed an anti-adhesive thermally cross-linked gelatin film. OBJECTIVE: We tried to clarify the re-attachability of the film and the required properties concerning the film thickness, stiffness and anti-adhesion effect. METHODS: To determine the optimal thickness, 5 kinds of the thickness of gelatin film and the conventional film were analyzed by the tensile test, shearing test, buckling test and tissue injury test. Finally, using the optimal film thickness, we tried to clarify the anti-adhesion effect of the reattached film. RESULTS: The tensile and shearing test showed gelatin films ≥30 μm thick had greater tensile strength and a smaller number of film fractures, than the conventional film. The buckling and tissue injury test showed gelatin films ≥60 μm thick had higher buckling strength and worse injury scores than the conventional film. The anti-adhesive effect of re-attached gelatin film using optimal thickness (30–40 μm) found the anti-adhesion score was significantly better than that of the control. CONCLUSIONS: Provided it has an optimal thickness, gelatin film can be reattached with enough physical strength not to tear, safety stiffness not to induce tissue injury, and a sufficient anti-adhesion effect.
Some aspects of the occurrence and development of chronic aseptic pododermatitis in highly pregnant cows
Authors: Khomyn N. M. , Mysak A. R. , Tsisinska S. V. , Pritsak V. V. , Nazaruk N. V. , Lenjo Yu. M. 
Published: November 2020

Pododermatitis in tall cows that occur on the background of secondary osteodystrophy can lead to significant economic losses, consisting of forced culling of animals, reduced milk yield, reproductive function and not enough receiving of calves. Therefore, the question of studying the peculiarities of origin and development, in particular chronic aseptic pododermatitis in these animals is quite actual. It is defined that with the same type of highly concentrated feeding, hypodynamics and insufficient insolation in the organism of highly pregnant cows, the process of assimilation of feed minerals is disrupted (develops, as is known, for dysfunction of the endocrine glands due to prolonged ketosis), which is appeared by resorption of the hoof bone, low rates of mass-volume extraction analysis of bone biopsy and testifies a systemic violation of mineral metabolism in animals and is confirmed by biochemical parameters, in particular, the concentration in the hoof horn of individual macro-and trace elements, as well as biophysical parameters of the epidermis of the hooves. Thus, the concentration of Calcium in the hoof horn is 16.8 % lower than in clinically healthy animals, Sulfur – by 20.3 %, Copper – by 16.2, Zinc – by 15.0, Cobalt – by 13.0 with increasing concentration of sulfhydryl groups by 34.3 % and moisture, respectively – by 5.5 %, which causes a decrease in the density and hardness of the epidermis of the hooves by 5.4 % and 19.0 %, respectively, and contributes to the deterioration of the quality of the hooves of highly pregnant cows. Such changes lead to deformation of the hooves, violation of the uniform load on their soles and the development of aseptic inflammation of the skin base of the hooves of a chronic nature, because the process of metabolic disorders occurs over a long period of time.
Morphological composition of blood and clinical indicators in ball fattening on the effect of b vitamins (В1, В2, В5, В6, В10, В12)
Authors: Golovach P. I. , Zmiya M. M. , Pokotylo S. O. 
Published: November 2020

In realization of the genetic productivity potential of different species of farm animals, an important place is given to full feeding. Insufficient supply of farm animals with individual vitamins has negative impact on the activity of the relevant enzyme systems, hormonal status, metabolism of nutrients, the state of the natural resistance of the various organs and organ systems, the processes of adaptation and productivity level. Numerical searches have shown that farm animals need in different vitamins depends on the type, age, sex, physiological state, the season, the level of productivity and others. According to some reports ruminants have been providing with water-soluble B vitamins by their rumen microbial synthesis accordingly it was recommended to rations setting for cattle, sheep and goats, along with nutrients and minerals only by carotene and vitamins D and E. Specific features of the effect of different doses of complex B vitamins (thiamine hydrochloride, riboflavin, niacin, pyridoxine hydrochloride, folic acid, ciankobalamin in venous blood) on morphological composition (erythrocytes, leukocytes, platelets, hemoglobin, hematocrit, erythrocyte sedimentation rate) and clinical indicators (heart rate, respiratory rate, type temperature) at the final stage bull fattening. Studies have shown that the addition to the diet of calves for fattening balanced nutrients and minerals and fat-soluble vitamins A, D, E complex of B vitamins (В1, В2, В5, В6, В10, В12) in appropriate doses generally positive effect on erythrocytes, leukocytes, hemoglobin, hematocrit, dose-dependent additionally introduced into the diet of B vitamins (В1, В2, В5, В6, В10, В12). The basis for our dosage of various B vitamins for Bovine experimental groups on fattening is the corresponding percentage of the recommended doses of certain B vitamins for fattening pigs (10 % – D1, 20 % – D2, 40 % – D3, 60 % – D4 group). The biggest change in terms on morphological composition in animals 3rd ((В1 – 0,040; В2 – 0,06; В5 – 1.2; В6 – 0.25; В10 – 0.0030; В12 – 0.0006 mg/kg body weight) and 4th (В1 – 0.070; В2 – 0.10; В5 – 2.0; В6 – 0.40; В10 – 0.0050; В12 – 0.0010 mg/kg body weight) research groups, and smallest – in bull 1st (В1 – 0.015; В2 – 0.03; В5 – 0.5; В6 – 0.10; В10 – 0.0012; В12 – 0.0002 mg/kg body weight) and 2 th (В1 – 0.025; В2 – 0.04; В5 – 0.8; В6 – 0.15; В10 – 0.0020; В12 – 0.0004 mg/kg body weight) research group, which is associated with dose introduced to the diet of calves during the final fattening phase of B vitamins (В1, В2, В5, В6, В10, В12).
The effectiveness of methods for laboratory diagnosis of otodectosis in dogs
Authors: Korchan L. , Zamaziy A. 
Published: November 2020

Otodectosis in domestic dogs is caused by ectoparasites of the species Otodectes cynotis (Hering, 1838), which parasitize on the inner surface of the auricles, the external auditory canal and the eardrum. The invasion is accompanied by severe itching, otitis, anxiety of animals, and sometimes – their death due to complications. The effectiveness of measures to combat otodectosis in dogs largely depends on the correct and accurate diagnosis of the invasion. At the same time, existing laboratory methods have different diagnostic efficiency. The aim of the study was to establish the effectiveness of mortal and vital methods of lifelong diagnosis of otodectosis in dogs. The effectiveness of modern methods of laboratory diagnosis of live and dead ticks of the species O. cynotis in dogs has been established. The most effective methods have been identified, the use of which will improve the quality of acarological research. Studies have shown that the most effective method of mortal methods was A. M. Titarenko, where a mixture of 50% aqueous solution of glycerin, dimethyl sulfoxide and 10% sodium hydroxide solution (the intensity of the invasion was 5.4 specimens of mites) was used as an illuminator. This method exceeded the intensity of invasion using the compressor method – 2 times (2.7 specimens of mites, P < 0.001), the flotation method – 1.2 times (4.5 specimens of mites). At the same time, the flotation method for otodectosis in dogs was 1.7 times more effective (P < 0.001) than the use of the compressor method. Of the vital methods of laboratory diagnosis of otodectosis in dogs, the most effective was the method using vegetable oil as a scraper clarifier, the intensity of the invasion was 5.9 copies. ticks. The use of this technique in terms of the number of detected otodectes exceeded the method of A.V. Alfimova (influence of elevated temperature) – 2.1 times (2.8 specimens of mites, P < 0.001) and the method of M.G. Khatin (centrifugation under the influence of elevated). temperature) – 1.3 times (4.6 specimens of mites, P < 0.001). In addition, the congratulatory method of M. G. Khatin in the diagnosis of otodectosis was 1.6 times more effective (P < 0.001) than the method of A. V. Alfimova.
Physical and Chemical Properties and Quality Control Methods of Hyaluronic Acid (Review)
Authors: Amandusova A. Kh. , Savelyeva K. R. , Morozov A. V. , Shelekhova V. A. , Shestakov V. N. , Persanova L. V. , Polyakov S. V. 
Published: November 2020

Introduction. This review describes the physicochemical properties that determine the use of hyaluronic acid in ophthalmology. We have studied methods for determining hyaluronic acid using various analytical methods.Text. Hyaluronic acid is a high molecular weight glycosaminoglycan that consists of repeating disaccharides of N-acetylglucosamine and D-glucuronic acid. Carboxyl, hydroxyl and acetoamide groups give hydrophilic properties to the molecule of this anionic heteropolysaccharide. Depending on how the hyaluronic acid is obtained, its molecular weight varies over a wide range. Researchers developed methods for controlling hyaluronic acid, which include the turbidimetric titration method, the method of high-performance capillary electrophoresis and high-performance liquid chromatography and IR spectroscopic method.Conclusion. Due to its properties, hyaluronic acid is widely used as an active ingredient in pharmaceutical preparations. Today, there are a number of methods for the determination of hyaluronic acid, including the method of turbidimetric titration, the method of capillary electrophoresis. High performance liquid chromatography (HPLC) and IR spectroscopy methods are presented in the Japanese Pharmacopoeia and the European Pharmacopoeia. These techniques are widely used due to their high reproducibility, accuracy, and relative simplicity.
N-nitrosamine Impurities in Medicines: Toxicity, Formation Pathways, Methods of Determination, and Limits (Review)
Authors: Shchetinin P. P. , Senchenko S. P. , Gordeev K. K. 
Published: November 2020

Introduction. N-nitrosamine compounds are potent genotoxic agents in animal species and some are classified as probable human carcinogens. This group of genotoxic impurities was found in drugs such as angiotensin II receptor blockers, histamine H1 receptor antagonists, and synthetic antidiabetic drugs. This discovery caused a flurry of alarm in the global pharmaceutical industry and resulted in a series of international investigations trying to determine root causes of nitrosamine formation in medicinal products and to find ways to minimize risks associated with nitrosamine contamination.Text. This paper provides an overview of the current state of the problem. It summarises the main pathways of N-nitrosamine formation in medicinal products at the stages from synthesis of active pharmaceutical ingredients to storage of finished pharmaceutical products. The paper describes the main mechanism responsible for the toxic effect of this group of impurities in human body. It also describes methods of extraction and analysis of N-nitrosamines found in medicinal products. It was demonstrated that high-performance liquid chromatography and gas chromatography-mass spectrometry are a golden standard for the detection of these contaminants. The paper also touches upon the main principles of setting limits for nitrosamine impurities in medicinal products.Conclusion. The data presented give a picture of the root causes of N-nitrosamine formation in medicinal products, as well as current detection and control methods used worldwide. Meanwhile, the paper raises a key issue about the need to develop Russian standards that would control the purity of medicinal products in terms of N-nitrosamine impurities. For that end, it will be necessary to draw on the experience of the leading USA and EU regulatory authorities.
Neutral Lipids of Oats Fruit (Avena Sativa L.)
Authors: Yuldasheva N. K. , Gusakova S. D. , Nurullaeva D. Kh. , Farmanova N. T. , Zakirova R. P. , Kurbanova E. R. 
Published: November 2020

Introduction. Lipids are a widespread group of biologically active substances in nature, making up the bulk of the organic substances of all living organisms. They accumulate in plants in seeds, as well as in fruits and perform a number of vital functions: they are the main components of cell membranes and the energy reserve for the body.Aim. Study of neutral lipids of sown oats (Avena sativa L.).Materials and methods. The objects of the study were fruits (grains) of oats of the sown variety "Tashkent 1," harvested in the Republic of Uzbekistan. Results and discussions. Neutral lipids of oat grains have been found to contain 13 fatty acids with a predominance of the sum of oleic, linolenic and linoleic acids. The total degree of unsaturation was almost 78%. Absorption bands characteristic of these substances were observed in the IR spectrum of MEGC.Conclusion. According to the results of the NL analysis, oat grains consisted of triacylglycerides and free LCDs, which were accompanied by hydrocarbons, phytosterols, triterpenoids and tocopherols.
Development and Validation of Dimeric Macrocyclic Tannin Assay Method in Dosage Forms
Authors: Ignаteva E. V. , Yartseva I. V. , Shprakh Z. S. , Prosalkova I. R. , Sasov S. A. , Orlova O. V. 
Published: November 2020

Introduction. Quantitative assessment of the active substance is necessary and perhaps the most significant part of the drug quality control. Validation of the analytical methods of quantitative assessment ensures their compliance with high requirements. The present study describes the development and validation of a spectrophotometry method for the quantitative evaluation of the active substance in the drug form of the national antitumor and antiangiogenic drug «Dimeric macrocyclic tannin (DMT) lyophilizate for solution for injection, 100 mg».Aim. The development and validation of the assay method for the standardization of «DMT lyophilizate for solution for injection, 100 mg».Materials and metods. The study used «DMT lyophilizate for solution for injection, 100 mg» and the active substance DMT. Method – spectrophotometry.Results and discussion. The methodof the quantitative assessment of the active substance in the DMT lyophilized drug by direct UV spectrophotometry was developed and the validation characteristics of the method were defined as a result of the study.Conclusion. The validation results showed that the assay method of DMT in the drug form has the appropriate accuracy, precision and linearity. The obtained results correspond to the approved criteria that allow the use of the developed methodology for evaluating the quality of the drug.
O que falar para a família? A predisposição para o câncer infantil hereditário
Authors: Cebulski Jenifer Nascimento da Silva , Couto Leila Leontina do 
Published: November 2020

Objetivo: Identificar o conhecimento de familiares sobre a doença infantil com predisposição hereditária ao câncer e analisar como a informação da doença é difundida no cenário intrafamiliar. Metodologia: Pesquisa qualitativa, exploratória. A coleta de dados foi desenvolvida com 16 familiares de crianças matriculadas no ambulatório de aconselhamento genético, através de entrevista individual, semi-estruturada com análise temática. Resultados: O desconhecimento sobre a doença com predisposição hereditária esteve presente no discurso dos familiares. Foram identificadas algumas estratégias de comunicação desenvolvida pelos familiares para a criança e familiares distantes. As informações obtidas no aconselhamento genético influenciaram na identificação de familiares de risco hereditário e cuidados com gerações futuras. Conclusões e implicações para a prática: A comunicação esteve presente no ambiente familiar com a criança e familiares diante da doença com predisposição hereditária ao câncer. Os profissionais de saúde necessitam se capacitar na área de oncologia genética para contribuir na identificação de crianças e famílias afetadas. As informações adquiridas no ambulatório de aconselhamento genético contribuem para o rastreamento de casos familiares, difusão da informação e na qualidade de vida dos afetados.
Transtorno de processamento sensorial e os prejuízos no processo de aprendizagem de alunos com transtornos do espectro autista: Um recado para os professores
Authors: Bacaro Paula Edicléia França , Mori Nerli Nonato Ribeiro 
Published: November 2020

A aprendizagem e o desenvolvimento da criança com Transtorno do Espectro do Autismo (TEA) estão atrelados a diversas situações que envolvem as funções psíquicas, físicas, emocionais e estruturais do desenvolvimento humano. Uma delas está relacionada ao Transtorno de Processamento Sensorial que pode prejudicar a aprendizagem das crianças com TEA. Para que a aprendizagem ocorra, com qualidade esperada, além de outros fatores, é necessário a harmonização das informações que chegam ao cérebro e transformam-se em respostas adaptativas esperadas. Quando esse processo não ocorre com qualidade, são geradas respostas insatisfatórias; há, então, a disfunção sensorial, cuja característica é o prejuízo na detecção, na modulação ou na interpretação dos estímulos sensoriais. Esse prejuízo pode provocar comportamentos inadequados, os quais reduzem e impedem a participação qualitativa da criança nas atividades pedagógicas. A fim de entender essa problemática foi realizada uma intervenção pedagógica com cinco crianças com TEA, em processo de aquisição da leitura e escrita, em situações de carga sensorial baixa e alta. Os resultados da pesquisa, qualitativa do tipo intervenção pedagógica, demonstraram que é possível reduzir a carga sensorial e consequentemente favorecer a aprendizagem das crianças. Assim, esse artigo tem o objetivo de enfatizar a importância do professor considerar o Transtorno de Processamento Sensorial em alunos com TEA, na elaboração das atividades pedagógicas e na sua prática de ensino, a fim de amenizar os prejuízos causados por ele na aprendizagem. Neste contexto destaca-se que cada criança com TEA apresenta uma condição específica de aprendizagem que exige do professor o conhecimento de novas práticas.
Atividade larvicida frente Aedes aegypti do óleo essencial das folhas secas de Syzygium cumini (L.) Skeels (Myrtaceae)
Authors: Everton Gustavo Oliveira , Rosa Paulo Victor Serra , Araújo Neto Ari Pereira de , Farias Wilma Karlla dos Santos , Conceição Francilidia Oliveira Vitorino de Assunção , Soares Liane Batista da Cruz , Lages Maria Gizelda Gomes , Pinheiro Feliciana Santos , Sousa Ricardo Teixeira de , Neves Ione Rocha , Lopes Thayson Rodrigues , Mouchrek Filho Victor Elias 
Published: November 2020

Este estudo objetivou avaliar a atividade larvicida do óleo essencial (OE) extraído das folhas secas de Syzygium cumini (L.) Skeels. Para extração do OE foi utilizada a técnica de hidrodestilação em um sistema extrator de Clevenger modificado. Os constituintes químicos foram obtidos por Cromatografia Gasosa acoplada a espectrometria de massas (CG/EM). Para atividade larvicida submeteu-se larvas de Aedes aegypti a soluções do OE em concentrações de 10-100 mg L-1, onde avaliou-se a mortalidade das larvas e determinou-se a CL50. O isocariofileno foi quantificado como majoritário no OE. Observou-se ação do OE como larvicida onde foi determinada a CL50 de 29,58 mg L-1. Os resultados obtidos permitiram concluir que o OE estudado apresentou atividade larvicida eficiente contra as larvas de Aedes aegypti, sendo importante e incentivado seu uso e aplicação.
Uma experiência interdisciplinar no ensino da Matemática: a construção de câmara escura no 9º ano do Ensino Fundamental
Authors: Nascimento Cláudia Brasil Coimbra , Lima Emanuelle São Leão de , Silva Thaís Deleprani Mansano da , Oliveira Alexandre Lopes de , Pereira Grazielle Rodrigues 
Published: November 2020

O presente artigo trata do relato de uma experiência realizada em sala de aula, durante a disciplina de Matemática em três turmas do 9º ano do Ensino Fundamental, de uma escola pública no Rio de Janeiro. A partir da construção de câmaras escuras pelos alunos, buscou-se associar o fenômeno físico ao ensino dos conceitos matemáticos de homotetia e semelhança de figuras. Após a etapa de construção e observação, os alunos fizeram relatórios para avaliarem as atividades. Ao longo da atividade experimental os participantes apresentaram diferentes questionamentos acerca do fenômeno observado, assim como perceberam a correlação entre as figuras semelhantes e os conceitos da óptica geométrica. Quanto a avaliação dos estudantes, nos relatórios eles destacaram a importância da atividade proposta como uma forma de fomentar o trabalho em equipe e a relevância da prática para o processo de ensino-aprendizagem. Por fim, nota-se que o interesse dos alunos pela atividade, assim como a construção do conceito a partir dos questionamentos demonstraram a importância da estratégia adotada para o ensino da Matemática.
DNA Repair-Based Gene Expression Signature and Distinct Molecular Subtypes for Prediction of Clinical Outcomes in Lung Adenocarcinoma
Authors: Hu Bin , Liu Di , Liu Yinqiang , Li Zhixi 
Published: November 2020

Objective: To conduct a robust prognostic gene expression signature and characterize molecular subtypes with distinct clinical characteristics for lung adenocarcinoma (LUAD).Methods: Based on DNA repair genes from the GSEA database, a prognostic signature was conducted in the TCGA-LUAD training set via univariate and multivariate cox regression analysis. Its prediction power was validated by overall survival analysis, relative operating characteristic (ROC) curves and stratification analysis in the GSE72094 verification set. Involved pathways in the high- and low-risk groups were analyzed by GSEA. A nomogram was built based on the signature and clinical features and its performance was assessed by calibration plots. LUAD samples were clustered via the ConsensusClusterPlus package. The differences in clinical outcomes, single nucleotide polymorphism (SNP) and sensitivity to chemotherapy drugs between molecular subtypes were analyzed.Results: A 13-DNA repair gene-signature was constructed for LUAD prognosis. Following validation, it can robustly and independently predict patients' clinical outcomes. The GSEA results exhibited the differences in pathways between high- and low- risk groups. A nomogram combining the signature and stage could accurately predict 1-, 3-, and 5-year survival probability. Two distinct molecular subtypes were characterized based on DNA repair genes. Patients in the Cluster 2 exhibited a worse prognosis and were more sensitive to common chemotherapy than those in the Cluster 1.Conclusion:This study proposed a 13-DNA repair gene-signature as a prognostic factor for LUAD patients, which can independently predict clinical outcomes by complement of the stage. Moreover, we characterized two LUAD subtypes with distinct clinical outcomes, somatic gene mutations, and drug sensitivity in cancer based on DNA repair genes.