bluuebunny/crossref_metadata_2025_split huggingface dataset:
Molecular Detection of blaoxa-23 Gene from Carbapenem Resistant Acinetobacter Baumannii Isolated from a Tertiary Care Hospital of Nepal
Authors: Neupane Laxmi , Shah Anil Kumar , Rayamajhee Binod , Singh Anjana 
Published: September 2020

Abstract
Background: Infections associated with Acinetobacter baumannii are increasing in many parts of the world especially the healthcare associated infections (HAIs). Antibiotics resistance is a great concern of public health which is either an inherent or adaptation property of microorganisms to resist the action of antibiotics. Carbapenem resistance, mainly among gram-negative bacteria is an ongoing problem that causes serious infections and dramatically limits the treatment alternatives. The prospective cross-sectional study was designed to detect the blaOXA-23 gene from carbapenem-resistant A. baumannii isolates in a tertiary care hospital of Nepal. Methods: A total of 380 clinical specimens (tracheal aspirate, urine, sputum, blood and wound samples, Foleys tips, and catheter tips) were collected from the study population and were examined by microbiological procedures including Gram’s staining, culture, and various biochemical tests. Antibiotic susceptibility testing (AST) was done as per the protocol of Kirby-Bauer disk diffusion technique and the CLSI guidelines while screening of carbapenemase production was checked through Modified Hodge Test (MHT) using Meropenem (10µg) disc. All the phenotypically positive results for carbapenemase production were further analysed by PCR and agarose gel electrophoresis for molecular detection of the blaOXA-23 gene. Results: Among 380 specimens analysed, 55.3% (210/310) of samples were positive for bacterial growth where 15.7% (33/210) of bacterial isolates were A. baumannii and 69.7% (23/33) were carbapenem-resistant. High prevalence (21.2%) of A. baumannii was among the patients of age group 51-60 followed by 41-50 years (18.2%) years but the result was statistically insignificant (P>0.05) and more isolates were from ICU (60.6%) followed by post-operative patients (18.2%) The blaOXA-23 carbapenemase gene was found in 82.6% (19/23) of meropenem resistant isolates while 97% isolates of A. baumannii were susceptible to colistin. Conclusion: The high rate of antibiotic resistance is funnelling the therapeutic options for the treatment of infections associated with A. baumannii which clearly shows a need for rational use of antibiotics. Systematic network surveillance should be established for monitoring and controlling the spread of the antibiotic-resistant gene of pathogenic bacteria especially in a resource-limited clinical setting like Nepal.
Left Atrial Dysfunction in Cardiac Amyloidosis and Hypertrophic Cardiomyopathy
Authors: Cui Shanshan , Wang Yueheng , Bai Hui 
Published: September 2020

Abstract
This study aims to explore the changes of left atrial function in patients with cardiac amyloidosis (CA) by speckle tracking echocardiography (STE) and identify the difference of left atrial properties between patients with CA and those with hypertrophic cardiomyopathy (HCM). In this study, 16 patients with CA, 16 patients with HCM, and 16 age-matched healthy controls were enrolled. The time-dependent strain parameters (LASr, LAScd, and LASdt) and strain rate parameters (m-SRs, m-SRe, and m-SRa) of left atrial function were measured by STE in patients with CA, then were compared with that in patients with HCM. Compared with the control group, CA group showed significantly reduced LVEDV/BSA, LVESV/BSA, A, and GLS of the left ventricle, and significantly increased heart rate, IVSd, IVPWd, E, E/A, E/e', LAd and LAV/BSA. The left atrial reserve (LASr and m-SRs), conduit (LAScd and m-SRe) and pump (LASdt and mSRa) functions of CA group were significantly reduced compared with that of controls (P<0.05). The left atrial reserve and pump functions of CA group were significantly reduced compared with that of HCM group. In the CA group, the left atrial reserve function (LASr and m-SRs), conduit function (LAScd and m-SRe) and pump function (LASct and m-SRa) were all related to left ventricular GLS and E/e'. The reserve function, conduit function and pump function of left atrial in the CA group decreased significantly than that in the health control group. When comparing with HCM group, CA group showed reduced reserve function and pump function of left atrial. STE is a qualified technique for the diagnosis of CA.
Biweekly Raltitrexed in Combination with Irinotecan (RIR) as Second-Line Therapy for Patients with Metastatic Colorectal Cancer: A Non-Randomized, Open-Label Phase II Trial.
Authors: Cheng Ke , Zhou Yu-Wen , Chen Ye , Li Zhi-Ping , Qiu Meng , Liu Ji-Yan 
Published: September 2020

Abstract
Background Irinotecan-based doublet chemotherapy strategy was standard second-line backbone treatment for patients with oxaliplatin‑refractory metastatic colorectal cancer(mCRC). The aim of this study was to evaluate tolerability and efficacy of raltitrexed combined with irinotecan biweekly administered as the second-line therapy for mCRC patients.Methods The study was a single-center, non-randomized, open-label phase II trial. Patients with mCRC after failure with first-line treatment of oxaliplatin and fluoropyrimidine or its derivatives were enrolled. Irinotecan (180 mg/m2) and raltitrexed (2.5 mg/m2) were given intravenously on day 1. Cycles were repeated every 2 weeks. The primary endpoint was progression-free survival, and the secondary endpoints included overall response rate, disease control rate, overall survival and treatment related adverse events. Results Between December 2012 and October 2016, 35 patients were enrolled. 33 and 35 patients were assessed for response and safety, respectively. The overall response rate (ORR) was 8.6 %, and the disease control rate (DCR) was 71.4%. The median progression-free survival (PFS) was 4.5 months (95% CI 3.8–5.2). The median overall survival was 12.0 months (95% CI 8.5–15.5). Four patients received conversion therapy to no evidence of disease (NED), and 2 patients were still alive with beyond 24 months survival. The most common grade 3/4 hematological adverse events were leukopenia (8.6%), neutropenia (5.7%). The most common grade 3/4 nonhematological adverse events were anorexia (14.3%), vomiting (14.3%), nausea (11.4%) and fatigue (8.6%). Two patients discontinued the protocol treatment because of treatment-related gastrointestinal adverse events. No one died from treatment-related events. The incidence and severity of toxicity was irrelevant to UGT1A1 status.Conclusions The combination of irinotecan with raltitrexed is an active, convenient and acceptable toxic regimen for second-line treatment for mCRC patients, which needs further study as a chemotherapy backbone to be combined with targeted agents in mCRC.Trial registration No. ChiCTR-ONC-12002767. The study was registered with the Chinese Clinical Trial Registry at 29 Octorber 2012, http://www.chictr.org.cn/index.aspx.
Blood Cultures and Broad-Spectrum Antimicrobials are Essential for Patients with Out-of-Hospital Cardiac Arrest
Authors: Lee Ching-Chi , Chen Po-Lin , Hong Ming-Yuan , Lin Chih-Hao , Ko Wen-Chien 
Published: September 2020

Abstract
Background: Etiologies of out-of-hospital cardiac arrest (OHCA) have been majorly focused on cardiac origins. Little is known regarding the role of bloodstream infections (BSIs) in OHCA episodes. Our aim was to disclose clinical features and incidences of BSIs and the survival benefit of prompt administration of appropriate antimicrobial therapy (AAT) in adults with OHCA.Methods: In the 10-year multicenter cohort, clinical information was retrospectively collected and causative microorganisms were prospectively stored for species identification and susceptibilities. The effect of delayed AAT administration on 30-day mortality was examined after adjustment for independent predictors of mortality, recognized by a multivariate regression analysis.Results: Of 1,021,177 emergency department encounters, OHCA visits had a higher BSI incidence than non-OHCA visits (413/3,429, 12.0% vs. 7,429/242,302, 3.1%; P<0.001). Compared with the matched non-OHCA (2,478) patients, OHCA (413) patients experienced more bacteremic episodes due to lower respiratory tract infections, fewer urosepsis events, fewer Escherichia coli bacteremia, and more streptococcal and anaerobes bacteremia. More antimicrobial-resistant organisms, such as methicillin-resistant Staphylococcus aureus and extended-spectrum beta-lactamase-producing Enterobacteriaceae, were evident in OHCA patients. Notably, each hour delay in AAT administration was associated with an average increase of 0.8% in crude 30-day mortality rates (adjusted odds ratio [AOR], 1.008; P = 0.04) in OHCA patients, 7% (AOR, 1.007; P < 0.001) in critically ill patients without OHCA, and 3% (AOR, 1.003; P < 0.001) in less critically ill patients.Conclusions: BSIs should be considered in patients experiencing OHCA; bacteremia sources, causative microorganisms, and antimicrobial susceptibilities differed between the OHCA and non-OHCA patients. The incorporation of blood culture samplings and rapid initiation of broad-spectrum antimicrobial therapy as the first-aids is essential for OHCA patients.
The new approach to the assessment of hydrological design limits in engineering prospecting of the forest zone
Authors: Белоногова Н.А. , Виноградов А.Ю. , Генсиоровский Ю.А. , Казаков Н.А. , Кузьмин Н.А. , Минаев А.Н. , Никифоровский А.А. , Салминен Э.О. 
Published: September 2020

В область интересов лесного хозяйства входят задачи обеспечения безопасности природных территорий, промышленных и жилых объектов, что может быть решено за счет безаварийного функционирования расположенных на них инженерных и гидротехнических сооружений. Затраты на строительство и эксплуатацию дорожных водопропускных сооружений напрямую зависят от результатов предпроектных расчетов возможной максимальной водности пересекаемых рек. Рассматриваются проблемы существующих гидрологических проектных ограничений и расчетных методик. Ставится под сомнение правомочность использования соответствующего математического аппарата, дается обоснование альтернативной системы таких ограничений. Приводится конкретный пример неоправданного завышения расчетных расходов воды для сооружения первого уровня ответственности, что является наиболее наглядным с точки зрения сравнительной оценки существующего и предлагаемого подходов к системе проектных ограничений. В качестве возможной альтернативы существующему подходу предложена расчетная схема. Установлено, что для малых водосборов, расположенных в центральной части зоны смешанных лесов, отношение максимального измеренного расхода каждой реки за период наблюдений к среднему максимальному не превышает 4. Отношение расчетного максимального вероятного паводка к среднему максимальному для рассматриваемой природной зоны - 10. Следовательно, в пределах, ограниченных этими значениями коэффициентов, в зависимости от ответственности лесохозяйственного сооружения и должна меняться расчетная величина максимального расхода.
The interests of forestry management include the tasks of security ensuring of natural areas, industrial and residential properties. This requirement can be achieved through the trouble-free operation of hydraulic engineering constructions located in this territory. The costs of construction and operation of road culverts depend on the results of calculations of the maximum water content of the crossed rivers. The article deals with the problems of the existing hydrological design constraints and calculation methods. The legitimacy of using the appropriate mathematical apparatus is under doubt. We justify an alternative system of such restrictions. There is a specific example of an unjustified overstatement of calculated water flow for the construction of the first level of responsibility. It is evident from the perspective of comparative assessment of the existing and proposed approaches to system of design constraints. The maximum measured river flow divided by the average of the maximum flow rate of less than 4. The estimated value of the probable maximum river flow divided by the average of the maximum flow rate of less than 10. The calculated value of the maximum flow rate should vary within these limits.
Organization of heterogeneous LAN with terminal access between its nodes
Authors: Хабаров С.П. 
Published: September 2020

Предложен подход к построению виртуальных гетерогенных ЛВС на базе физически однородных ЛВС, при котором на каждом узле физической ЛВС устанавливается среда виртуализации, а на сервере размещаются файлы виртуальных машин с необходимыми для работы в составе ЛВС гостевыми ОС. Показана возможность организации работы с удаленными рабочими столами в системах Ubuntu-Windows и Ubuntu-Ubuntu. Отмечено, что рабочий стол узла на базе ОС Windows блокируется при входе на его удаленный рабочий стол, а узел с ОС Ubuntu позволяет одновременно работать как на основном, так и на нескольких удаленных рабочих столах. Использование протокола RDP позволяет в системе Ubuntu-Windows удаленно конфигурировать и настраивать множество узлов ЛВС, а в ОС Ubuntu Server 14.04 - организовывать одновременный доступ нескольких пользователей на один сервер, используя для каждого из них свой удаленный рабочий стол. Это позволит в компьютерном классе на базе ОС Windows организовать работу в среде ОС Ubuntu
This article proposes an approach to the construction of heterogeneous virtual LAN on the basis of physically homogeneous LAN. To do this on each node physical LAN installs the virtualization environment and the server are downloaded files of virtual machines with guest operating systems that are required to work as part of the LAN. In addition, we show the ability to work with remote desktop in Ubuntu-Windows and Ubuntu-Ubuntu systems. It is noted that a desktop of Windows is blocked for working when the other user connects to his remote desktop. Unlike Windows, LAN node with OS Ubuntu can work simultaneously as a main desktop, so multiple remote desktops. The use of the RDP protocol in LAN allows Ubuntu-Windows systems remotely configure and customize the set of LAN nodes, at the same time OS Ubuntu Server 14.04 allows to organize simultaneous access multiple users to a single server, using for each of them the own remote desktop. This allows the computer class based on Windows, use for organizing the work in an environment OS Ubuntu.
TRASDISCIPLINARIDADE NO ENSINO E PESQUISA DA GERAÇÃO DE FORMAS ARQUITETÔNICAS
Authors: Períes Lucas , Silvina Barraud 
Published: September 2020

Na pesquisa apresentada neste artigo, é abordada a relação específica entre as ordens geométricas e a geração da forma arquitetônica contemporânea, com a intenção de fornecer ferramentas generativas que participem ativamente durante o processo de ensino e aprendizagem da morfologia. A disciplinaridade cruzada ou unidirecional é apresentada como uma condição das relações entre disciplinas e como uma abordagem que apela à procura de estruturas conceituais e técnicas em outros campos do conhecimento, aquelas com potencial para a sua transferência para a arquitetura. Neste contexto, a mistura é produzida em: leis, princípios, ferramentas e técnicas externas para a produção de conhecimento disciplinar integral.
A atenção é colocada na: física, matemática aplicada, hidrografia, ceraunología (parte da meteorologia que estuda os raios e seus fenômenos), neurologia e botânica, caracterizadas por favorecer a identificação de padrões com potencial de ensaio e sistematização através de práticas de indagação que contribuem para a geração de formas arquitetônicas. A natureza da pesquisa é qualitativa e o seu desenho metodológico é bibliográfico, exploratório e experimental. Numa primeira fase, é realizada a pesquisa documental e é especificada a classificação dos casos multidisciplinares. Numa segunda instância, o trabalho é colocado no âmbito da pesquisa projetual, e é experimentado a fim de produzir conhecimentos através da utilização do projeto e do seu processo de construção.
Recombinant Expression and Characterization of Lemon (Citrus limon) Peroxidase
Authors: Pandey Veda P. , Tyagi Apoorvi , Ali Shagoofa , Yadav Kusum , Yadav Anurag , Shasany Ajit K. , Dwivedi Upendra N. 
Published: September 2020

Background:
Class III plant peroxidases play important role in a number of physiological
processes in plants such as lignin biosynthesis, suberization, cell wall biosynthesis, reactive oxygen
species metabolism and plant defense against pathogens. Peroxidases are also of significance
in several industrial applications. In view of this, the production and identification of novel peroxidases
having resistance towards temperature, pH, salts is desirable.

Objective:
The objective of the present work was to clone and characterize a novel plant peroxidase
suitable for industrial application.

Methods:
A full length cDNA clone of lemon peroxidase was isolated using PCR and RACE approaches,
characterized and heterologously expressed in Escherichia coli using standard protocols.
The expressed peroxidase was purified using Ni-NTA agarose column and biochemically characterized
using standard protocols. The peroxidase was also in-silico characterized at nucleotide as well
as protein levels using standard protocols.

Results:
A full length cDNA clone of lemon peroxidase was isolated and expressed heterologously
in E. coli. The expressed recombinant lemon peroxidase (LPRX) was activated by in-vitro refolding
and purified. The purified LPRX exhibited pH and temperature optima of pH 7.0 and 50°C, respectively.
The LPRX was found to be activated by metal ions (Na+, Ca2+, Mg2+ and Mn2+) at lower
concentration. The expressional analysis of the transcripts suggested involvement of lemon peroxidase
in plant defense. The lemon peroxidase was in silico modelled and docked with the substrates
guaiacol, and pyrogallol and shown the favourability of pyrogallol over guaiacol, which is in agreement
with the in-vitro findings. The protein function annotation analyses suggested the involvement
of lemon peroxidase in the phenylpropanoid biosynthesis pathway and plant defense mechanisms.

Conclusion:
Based on the biochemical characterization, the purified peroxidase was found to be resistant
towards the salts and thus, might be a good candidate for industrial exploitation. The in-silico
protein function annotation and transcript analyses highlighted the possible involvement of the
lemon peroxidase in plant defense response.
FADS1 is a Prognostic Biomarker in Bladder Cancer: A Study Based on TCGA Data
Authors: Lu Ying , Shao Jing , Shu Xu , Jiang Yaofei , Rong Jianfang , Lai Yongkang , Liu Ji 
Published: September 2020

Aim and Objective:
Fatty acid desaturase 1 (FADS1) has been reported to be a potential biomarker in various
cancers. However, no study has explored the relationship between FADS1 expression and bladder cancer. Our study aimed
to investigate the role of FADS1 in bladder cancer prognosis via The Cancer Genome Atlas (TCGA).

Materials and Methods:
RNA-Seq expression of 414 tumor tissues and 19 paired normal tissues, as well as corresponding
clinical data, were downloaded from TCGA database. Two cancer cases were excluded due to a lack of clinical information.
The association between FADS1 and the clinicopathological features of bladder cancer was analyzed. This study was conducted in October of 2019 in China.

Results:
The high expression of FADS1 in bladder cancer was significantly related to histological grade (OR = 0.155 for
low vs. high), clinical stage (OR=2.074 for III or IV vs. I or II), T classification (OR=2.326 for T3 or T4 vs. T1 or T2), lymphatic metastasis (OR=1.923 for N1 or N2 or N3 vs. N0) and distant metastasis (OR=4.883 for yes vs. no) (all p-values
<0.05). Bladder cancer with high FADS1 levels was related to a worse prognosis than bladder cancer with low FADS1 levels (p= 1.626*10-5
), according to median expression value 3.622. FADS1 was an independent factor of overall survival in
bladder cancer, with a hazard ratio of 1.048 (95%CI: 1.020–1.077, p = 0.001).

Conclusions:
Increased FADS1 expression in bladder cancer is associated with advanced clinical pathological features and
may be a potential biomarker for poor prognosis.
Clinical Manifestations of Periodontal Tissue Diseases in Patients with Rheumatoid Arthritis Who Live in Anthropogenically Loaded Areas
Authors: Kovalyshyn Khristina , Rozhko Mykola 
Published: September 2020

The objective of the study is to clinically assess the condition of periodontal tissues in patients with rheumatoid arthritis, living in anthropogenically loaded and environmentally friendly areas and in people with generalized periodontitis without concomitant somatic diseases.
Materials and methods. There were examined 137 patients, including 82 patients with generalized periodontitis of the I degree (subgroups A) and the II degree (subgroups B) with rheumatoid arthritis, living in anthropogenically loaded areas (group I), environmentally friendly areas (group II) and without concomitant somatic diseases living in environmentally friendly areas (group III). Control group – included 18 healthy people.
Periodontal tissues were evaluated according to the indices: Greene-Vermillion, PMA, periodontal index offered by Russel and the depth of periodontal pockets.
Results. Women (80%) dominated in each group, patients with generalized periodontitis of the II degree of severity dominated, too.
The highest depth of periodontal pockets was 5.02±0.11 mm in the IB subgroup and differed significantly from this figure in the IIB subgroup 1.07-fold (pIB-IIB<0.05) and from that in the IIIB subgroup 1.2-fold (pIB-IIIB<0.001). The value of the Greene-Vermilion index in patients with GP of the I degree of development in all subgroups A corresponded to “unsatisfactory”. In patients with GP of the II degree of development, the state of hygiene corresponded to “bad” in subgroups IB, IIB and was 3.04±0.11 points (pIB-IIB˂0.01, pIB-IIIB˂0.001, pIB-K˂0.001); 2,63 ± 0.07 points (pIIB-IB˂0.01, pIIB-IIIB˂0.001, pIIB-K˂0.001), in ІІІB – “unsatisfactory”. According to the indicators of the PMA index in patients with GP of the I degree of severity, we’ve found the average severity degree of gingivitis (within the range of 43.25±2.02 – 48.06±1.46%) and severe degree of gingivitis in patients with the GP of the II degree >50 %. The highest indicator of periodontal index was found in the IB subgroup – 6.16±0.10 points (pIB-IIB˂0.01, pIB-IIIB˂0.001, pIB-K˂0.001), which indicated a severe form of periodontitis.
Conclusion. Most often, GP in patients with RA was diagnosed in women, most of whom were patients with GP of the II degree (most in group I – 69.04%). The highest depth of periodontal pockets was 5.02±0.11 mm in the IB subgroup. The performed clinical and index assessment of periodontal tissues in patients of three groups indicates a more severe course of GP of the I and II degree in patients with rheumatoid arthritis living in anthropogenically loaded areas (IA, IB subgroups).