bluuebunny/crossref_metadata_2025_split huggingface dataset:
Longitudinal spinous-splitting laminoplasty with coral bone for the treatment of cervical adjacent segment degenerative disease: A 5-year follow-up study
Authors: He Wei , Wang Qilong , Tian Wei , Han Bing , Wang Yumei , Liu Mingming 
Published: March 2020

Abstract
Background: To analyze the causes of cervical adjacent segment degenerative disease (ASDis), explore the surgical results of longitudinal spinous-splitting laminoplasty with coral bone (SLAC) during cervical reoperation, and accumulate data on reoperation with SLAC in a primary hospital.Methods: We conducted a retrospective study. From 1998 to 2014, 52 patients underwent cervical reoperation for ASDis using SLAC at our hospital. Among them, 39 were treated with anterior cervical fusion and internal fixation in the first operation (anterior cervical corpectomy with fusion [ACCF], n=24; anterior cervical discectomy and fusion [ACDF], n=11; and cervical disc arthroplasty [CDA], n=4).Results: In patients who underwent an anterior cervical approach in the first instance, ASDis was significantly higher in the C3/4 gap than in other gaps. In the ACCF group, the lateral radiograph of the cervical spine revealed that the distance between the anterior cervical plate and adjacent segment disc in15 cases (62.5%) was <5 mm, and five cases (12.8%) had internal fixation screws that broke into the annulus of the adjacent segment. After the first SLAC, ASDis occurred in C2/3 and C3/4 in four (30.8%) and eight cases (61.5%), respectively. Post-reoperation, all cases were follow-up for >5 (average, 6.2) years. Comparing pre-reoperation and last follow-up values, the mean Japanese Orthopedic Association score was 10.2±1.5 versus15.5±0.7 (P=0.03), neck disability index was 26.2 versus13.6 points (P=0.01), upper-limb visual analog scale (VAS) score was 6.1 versus2.6 points (P=0.04), and neck and shoulder VAS score was 6.6 versus 2.1 points (P=0.03).Conclusions: ASDis was primarily caused by 1) a distance of <5 mm between the anterior cervical plate and adjacent segment disc and 2) the screw breaking through the adjacent segmental annulus. SLAC proved to be a simple technique, with clear local anatomy and satisfactory clinical results.
The human arthritic hip joint is a source of mesenchymal progenitor cells (MPCs) with extensive multipotent differentiation potential
Authors: Wagenbrenner Mike , Heinz Tizian , Horas Konstantin , Jakuscheit Axel , Arnholdt Joerg , Herrmann Marietta , Rudert Maximilian , Holzapfel Boris , Steinert Andre , Weißenberger Manuel 
Published: March 2020

Abstract
Background: While multiple in vitro studies examined mesenchymal progenitor cells (MPCs) derived from bone marrow or hyaline cartilage, there is little to no data about the presence of MPCs in the joint capsule or the ligamentum capitis femoris (LCF) of the hip joint. Therefore, this in vitro study examined the presence and compared the differentiation potential of MPCs isolated from the bone marrow, arthritic hyaline cartilage, the LCF and full-thickness samples of the anterior joint capsule of the hip joint. Methods: MPCs were isolated and multiplied in adherent monolayer cell culture. Osteogenesis and adipogenesis was induced in monolayer cell cultures for 21 days using a differentiation medium containing specific growth factors, while chondrogenesis in the presence of TGF-ß1 was performed using pellet-culture for 27 days. Control cultures were maintained for comparison over the same duration of time. The differentiation process was analyzed using histological and immunohistochemical stainings as well as semiquantitative RT-PCR for measuring the mean expression levels of tissue-specific genes.Results: This in vitro research showed that the isolated cells from all four donor tissues grew plastic adherent and showed similar adipogenic and osteogenic differentiation capacity as proven by the histological detection of lipid droplets or deposits of extracellular calcium and collagen type I. After 27 days of chondrogenesis proteoglycans accumulated in the differentiated MPC-pellets from all donor tissues. Immunohistochemical staining revealed vast amounts of collagen type II in all differentiated MPC-pellets, except for those from the LCF. Interestingly all differentiated MPCs still showed a clear increase in mean expression of adipogenic, osteogenic and chondrogenic marker genes. In addition the examination of an exemplary donor sample revealed that cells from all four donor tissues were clearly positive for the surface markers CD44, CD73, CD90 and CD105 by flow cytometric analysis.Conclusions: This study proved the presence of MPCs in all four examined donor tissues of the hip joint. No significant differences were observed during osteogenic or adipogenic differentiation depending on the source of MPCs used. Further research is necessary to fully determine the chondrogenic differentiation potential of MPCs isolated from the LCF and capsule tissue of the hip joint.
Study on the effect of DCGS on planned dose with 3DVH-γ analysis and bio-mathematical model for cervical cancer
Authors: Bai Han , Zhu Sijin , Wu Xingrao , Liu Xuhong , Chen Feihu , Yan Jiawen 
Published: March 2020

Abstract
Objective: The 3DVH-γ analysis and bio-mathematical model were used to analyze the effect of the dose calculation grid size (DCGS) on the planned dose (PD) for cervical cancer to explore the efficacy and sensitivity of the two methods in detecting dose changes.Methods：17 patients’ plans for cervical cancer were enrolled（Pinnacle TPS，VMAT）, and the DCGS was changed from 2.0mm to 5.0mm to calculate the planned dose respectively. The dose distribution calculated by DCGS = 2.0mm as the “ reference ” data set (RDS) , the dose distribution calculated by the rest DCGS as the“measurement”data set (MDS), the 3DVH-γ passing rates and the (N)TCPs of the all structures under different DCGS were abtained , and then analyze the influences of DCGS on the γ passing rate and (N)TCPs and the relationship between their changes.Results: The effect of DCGS on planned dose was obvious. When the γ-standard was 1.0mm, 1.0% and 10.0%, the difference of the results of the DCGS on dose effect could be detected by 3DVH-γ analysis (ps<0.05). With the decline of the standard, 3DVH-γ analysis’ ability to detect this difference was also declining. When the standard was 1.0mm, 3.0% and 10.0%, the p value of >0.05 accounted for the majority. With DCGS=2.0mm being RDS, ∆γ-passing-rate presented the same trend with ∆(N)TCPs of all structures except for the femurs only when the 1.0mm, 1.0% and 10.0% standards were adopted for the 3DVH-γ analysis.Conclusions: The 3DVH-γ analysis and bio-mathematical model can be used to analyze the effect of DCGS on the planned dose, and the former’s detection ability has a lot to do with the designed standard, and the latter’s capability is related to the parameters and calculated accuracy of the latter.
A Modified Management of Implant Associated Postoperative Osteomyelitis With Cierny-Mader Type
Authors: Tan Yanbin , Li Hang , Pan Zhijun , Zheng Qiang 
Published: March 2020

Abstract
Background: No standardized protocol has been suggested in the treatment of postoperative osteomyelitis. Our team evaluates the clinical efficacy of the modified tactics of implant associated postoperative osteomyelitis patients according to Cierny-Mader type. Methods: 95wounds were reviewed from March 2009 to February 2016 in our hospital. 61 wounds were treated by the modified algorithm as following: Stable hardware + bone not healed Cierny–Mader 1 type = remove hardware, temporary stabilize; Stable hardware + bone not healed Cierny–Mader 2 type = retain hardware ; Stable hardware + bone not healed Cierny–Mader for type 3 and type 4 = remove hardware, temporary stabilize/ Ilizarov technique; Unstable hardware + bone not healed = remove hardware, temporary stabilize/ Ilizarov technique; Stable hardware + bone healed = remove hardware. 34 wounds were treated by the conventional algorithm. Autodermoplasty, flap transfer, myocutaneous flap and other methods including antibiotic irrigation and drug delivery system were used in wound repair. Results: The patients treated with modified algorithm had a significantly reduced recurrence (P＜0.01) , and increased results of negative bacterial cultures (P＜0.01), but however observed a decrease in the number of retained hardware cases (P＜0.05) . For those treated with tissue reconstruction, there was no significantly (P＞0.05) comparing with the conventional group. Conclusions: The modified technique for the implant associated postoperative osteomyelitis according to the stability of the hardware and Cierny-Mader type represent a good clinical efficacy in the management of postoperative osteomyelitis. This procedure is simple and shows promising results, more clinical evidence is needed to confirm the existing findings and optimize the treatment of postoperative osteomyelitis. Key Words: Osteomyelitis, Postoperation, Implant
A Didactic Intervention to Improve Fertility Knowledge Among Resident Physicians
Authors: Roberts Leah May , Kudesia Rashmi , Dolan Shaliz , Rose Marisa 
Published: March 2020

Abstract
Background: Knowledge of family planning and reproductive health is recommended as part of the Core Competencies for Internal Medicine (IM) trainees 1 , yet is rarely assessed. Objective: This pilot study explored the need for and efficacy of a didactic session to impact fertility knowledge among IM residents Methods: IM residents at a single institution were recruited via email to voluntarily attend a didactic session on fertility in March of 2018. Attendees of the session voluntarily completed a survey to assess demographics and fertility knowledge, measured by the validated Fertility & Infertility Treatment Knowledge Score (FIT-KS), both before (“pre-test”) and after (“post-test”) the intervention. Results: Of 108 possible residents, thirty residents attended the session (27%). Of those who attended eighteen completed the pre-test survey (60%), and ten completed a portion of the post-test survey (33%). There was no significant association between fertility knowledge and demographics. The mean pre-test FIT-KS score was 65.1% (+/- 9.1%). The post-test mean FIT-KS score was 70.3% (+/-8.8%). Seven participants (response rate of 6.5%) completed both the pre and post-test with their average matched score improving from 64% (+/- 8.2) to 72.4% (+/- 4.9) (p=0.047). In the paired analysis, the rates of feeling comfortable answering patient questions about fertility increased from 14% to 57%. Conclusions: Substantial gaps exist in fertility knowledge among IM residents, with understanding of male fertility being particularly limited. Despite a small sample size, the intervention significantly improved IM residents’ fertility knowledge and their comfort in discussing fertility with patients.
Analysis of clinical characteristics and laboratory findings of 95 cases of 2019 novel coronavirus pneumonia in Wuhan, China: a retrospective analysis.
Authors: Zhang Gemin , Zhang Jie , Wang Bowen , Zhu Xionglin , Wang Qiang , Qiu Shiming 
Published: March 2020

Abstract
Background: Since December 2019, 2019 novel coronavirus pneumonia emerged in Wuhan city and rapidly spread throughout China and even the world. We sought to analyse the clinical characteristics and laboratory findings of some cases with 2019 novel coronavirus pneumonia .Methods: In this retrospective study, we extracted the data on 95 patients with laboratory-confirmed 2019 novel coronavirus pneumonia in Wuhan Xinzhou District People's Hospital from January 16th to February 25th , 2020. Cases were confirmed by real-time RT-PCR and abnormal radiologic findings. Outcomes were followed up until March 2th , 2020. Results: Higher temperature, blood leukocyte count, neutrophil count, neutrophil percentage, C-reactive protein level, D-dimer level, alanine aminotransferase activity, aspartate aminotransferase activity, α - hydroxybutyrate dehydrogenase activity, lactate dehydrogenase activity and creatine kinase activity were related to severe 2019 novel coronavirus pneumonia and composite endpoint, and so were lower lymphocyte count, lymphocyte percentage and total protein level. Age below 40 or above 60 years old, male, higher Creatinine level, and lower platelet count also seemed related to severe 2019 novel coronavirus pneumonia and composite endpoint, however the P values were greater than 0.05, which mean under the same condition studies of larger samples are needed in the future. Conclusion: Multiple factors were related to severe 2019 novel coronavirus pneumonia and composite endpoint, and more related studies are needed in the future.
WITHDRAWN: Methylene blue photochemical treatment as a reliable SARS-CoV-2 plasma virus inactivation method for blood safety and convalescent plasma therapy for the COVID-19 outbreak
Authors: Jin Changzhong , Yu Bin , Zhang Jie , Wu Hao , Zhou Xipeng , Yao Hangping , Liu Fumin , Lu Xiangyun , Cheng Linfang , Jiang Miao , Wu Nanping 
Published: March 2020

Abstract

Background With the outbreak of unknown pneumonia in Wuhan, China in December 2019, a new coronavirus (SARS-CoV-2) attracted worldwide attention. Although coronaviruses typically infect the upper or lower respiratory tract, discovery of the virus in plasma is common. Therefore, the risk of transmitting coronavirus through transfusion of blood products remains. As more asymptomatic infections are found in COVID-19 cases, blood safety is shown to be particularly important, especially in endemic areas. Study Design and MethodsBX-1, an ‘AIDS treatment instrument’ based on methylene blue (MB) photochemical technology, developed by Boxin (Beijing) Biotechnology Development LTD, has proven that inactivation of lipid-enveloped viruses such as HIV-1 in plasma has high efficiency, without damage to other components in the plasma, and proved safe and reliable in clinical trials of HIV treatment. In order to confirm the inactivation effect of BX-1 in SARS-CoV-2, we used the SARS-CoV-2 virus strain isolated from Zhejiang University for plasma virus inactivation studies. Results and ConclusionBX-1 can effectively eliminate SARS-CoV-2 within 2 mins, and the virus titer decline can reach 4.5 log10 TCID50/mL. Faced with the expanding epidemic, BX-1 is safe for blood transfusion and plasma transfusion therapy in recovery patients, and the inactivated vaccine preparation has great potential for treatment in the current outbreak.
Molecular and morphological characterization of the alfalfa cyst nematode, Heterodera medicaginis, from Utah
Authors: Handoo Zafar A. , Skantar Andrea M. , Hafez Saad L. , Kantor Mihail R. , Hult Maria N. , Rogers Stephen A. 
Published: March 2020

Abstract
In the spring of 2019, a cyst nematode was discovered from soil samples collected from an alfalfa field in Millard County, Utah. The soil samples were submitted to one of us (SH), who extracted the nematode cysts and sent them to the USDA-ARS, Mycology and Nematology Genetic Diversity and Biology Laboratory (MNGDBL), Beltsville, MD for morphological and molecular identification. Cysts and living nematode juveniles (J2) recovered from the cysts were examined morphologically and molecularly for species identification which indicated that the specimens were Heterodera medicaginis. This represents the first record of H. medicaginis in Utah and the second report of this nematode in North America.
Molecular approach to confirm traditional identification of Radopholus similis sampled in Tanzania
Authors: Mgonja Doreen M. , Temu Gladness E. , Ndunguru Joseph C. , Mziray Magreth F. , Lyantagaye Sylvester L. , Luambano Nessie D. 
Published: March 2020

Abstract
Banana (Musa spp. L.) is an important staple food and cash crop for about 30% of the population in Tanzania; however, the burrowing plant-parasitic nematode Radopholus similis causes black head disease and toppling in banana plants, which results in yield losses. We collected and identified 80 specimens of R. similis from four agro-ecological zones in Tanzania using morphological characters. We then used universal and specific R. similis primers to amplify the small subunit, internal transcribed spacer and large subunit of ribosomal DNA regions of these specimens. The amplicons were subsequently sequenced and analyzed using Bayesian inference. We identified two major clades, one that comprised all R. similis sequences derived from this study and another that included R. similis and Radopholus spp. sequences obtained from GenBank, indicating the separation of this species from congeneric sequences. Our findings provide a useful, simple and rapid method for identifying burrowing nematodes. This outcome could contribute to the development of permanent, integrated pest management strategies for the control of R. similis in banana and other crops in order to reduce associated yield losses in Tanzania. To our knowledge, this is the first study of nematodes to use combined morphological and molecular methods for the identification of R. similis in Tanzania.
Genome sequence of the root-knot nematode Meloidogyne luci
Authors: Susič Nik , Koutsovoulos Georgios D. , Riccio Cristian , Danchin Etienne G. J. , Blaxter Mark L. , Lunt David H. , Strajnar Polona , Širca Saša , Urek Gregor , Stare Barbara Gerič 
Published: March 2020

Abstract
Root-knot nematodes from the genus Meloidogyne are polyphagous plant endoparasites and agricultural pests of global importance. Here, we report the high-quality genome sequence of Meloidogyne luci population SI-Smartno V13. The resulting genome assembly of M. luci SI-Smartno V13 consists of 327 contigs, with an N50 contig length of 1,711,905 bp and a total assembly length of 209.16 Mb.
Actividad física en adolescentes y su relación con agresividad, impulsividad, internet y videojuegos
Authors: Chaín-Pinzón Nicolás , Briñez Blanca 
Published: March 2020

El presente estudio aborda la relación existente entre la frecuencia de actividad física con las variables agresividad, impulsividad, internet y videojuegos. En la investigación participaron 254 adolescentes colombianos en edades comprendidas entre los 12 y 17 años (Md=14.18; Dt = 1.47). Se aplicaron tres instrumentos a saber, la Escala de Impulsividad Barratt para Niños (Bis 11-c), el Cuestionario de Agresividad de Buss & Perry (AQ) y el Cuestionario CAGE para Detección de Problemas de Internet y Videojuegos en Adolescentes. Los resultados señalan que existe una relación significativa entre la actividad física con el componente cognitivo de la impulsividad (rs=1.92, p<.001) y el uso de internet y videojuegos (rs =.142, p<.05). Asimismo se confirma una vez más las diferencias de género a favor de los hombres en la realización de actividad física en adolescentes (U=5555.0, p=.000). No se encontraron diferencias significativas en los grupos de edad (χ² (5) =7.42, p = .191), tampoco se hallaron correlaciones significativas con la agresividad, ni con los demás componentes de la impulsividad.
Spatial interpolation of rain-field dynamic time-space evolution based on radar rainfall data
Authors: Liu Peng , Tung Yeou-Koung 
Published: March 2020

AbstractAccurate and reliable measurement and prediction of the spatial and temporal distribution of rain field over a wide range of scales are important topics in hydrologic investigations. In this study, a geostatistical approach was adopted. To estimate the rainfall intensity over a study domain with the sample values and the spatial structure from the radar data, the cumulative distribution functions (CDFs) at all unsampled locations were estimated. Indicator kriging (IK) was used to estimate the exceedance probabilities for different preselected threshold levels, and a procedure was implemented for interpolating CDF values between the thresholds that were derived from the IK. Different probability distribution functions of the CDF were tested and their influences on the performance were also investigated. The performance measures and visual comparison between the observed rain field and the IK-based estimation suggested that the proposed method can provide good results of the estimation of indicator variables and is capable of producing a realistic image.
Molecular-level comparison of dissolved organic matter in 11 major lakes in Japan by Orbitrap mass spectrometry
Authors: Kasuga Ikuro , Yuthawong Vitharuch , Kurisu Futoshi , Furumai Hiroaki 
Published: March 2020

AbstractDissolved organic matter (DOM) causes organic pollution in lakes, resulting in the occurrence of off-flavour etc. when lake water is used as a drinking water source. In this study, DOM in 11 major lakes in Japan was characterised by high-resolution Orbitrap mass spectrometry. Molecular formulas were assigned to 845–1,451 components per sample. Among them, 555 components were commonly found in all lakes. The DOM compositions of the 11 lakes were clustered into four groups. Correlation analysis could extract specific components whose relative intensities were associated with water quality indices such as specific ultraviolet absorbance, specific dissolved chemical oxygen demand (DCODMn), and specific trihalomethane formation potential (R = 0.80–0.93, p &lt; 0.05). Although further molecular structural analyses of DOM components are necessary, these results could be informative for exploring key candidates related to specific water quality issues. Pre-treatment of samples with permanganate oxidation was applied to screen components which could contribute to DCODMn. DCODMn components accounted for only 7–30% of total peak intensities, indicating the limited performance of permanganate oxidation. Pre-treatment by permanganate coupled with Orbitrap MS revealed that components with higher molecular weight, higher oxygen-to-carbon ratios (O/C), and lower hydrogen-to-carbon ratios (H/C) could be responsible for DCODMn.
Setting New Routes for Antifungal Drug Discovery Against Pathogenic Fungi
Authors: Freitas e Silva Kleber S. , C. Silva Lívia , Gonçales Relber A. , Neves Bruno J. , Soares Célia M.A. , Pereira Maristela 
Published: March 2020

:Fungal diseases are life-threatening to human health and responsible for millions of deaths around the world. Fungal pathogens lead to a high number of morbidity and mortality. Current antifungal treatment comprises drugs, such as azoles, echinocandins, and polyenes and the cure is not guaranteed. In addition, such drugs are related to severe side effects and the treatment lasts for an extended period. Thus, setting new routes for the discovery of effective and safe antifungal drugs should be a priority within the health care system. The discovery of alternative and efficient antifungal drugs showing fewer side effects is time-consuming and remains a challenge. Natural products can be a source of antifungals and used in combinatorial therapy. The most important natural products are antifungal peptides, antifungal lectins, antifungal plants, and fungi secondary metabolites. Several proteins, enzymes, and metabolic pathways could be targets for the discovery of efficient inhibitor compounds and recently, heat shock proteins, calcineurin, salinomycin, the trehalose biosynthetic pathway, and the glyoxylate cycle have been investigated in several fungal species. HSP protein inhibitors and echinocandins have been shown to have a fungicidal effect against azole-resistant fungi strains. Transcriptomic and proteomic approaches have advanced antifungal drug discovery and pointed to new important specific-pathogen targets. Certain enzymes, such as those from the glyoxylate cycle, have been a target of antifungal compounds in several fungi species. Natural and synthetic compounds inhibited the activity of such enzymes and reduced the ability of fungal cells to transit from mycelium to yeast, proving to be promisor antifungal agents. Finally, computational biology has developed effective approaches, setting new routes for early antifungal drug discovery since normal approaches take several years from discovery to clinical use. Thus, the development of new antifungal strategies might reduce the therapeutic time and increase the quality of life of patients.