bluuebunny/crossref_metadata_2025_split huggingface dataset:
Wound hematoma after anterior cervical spine surgery: A retrospective study of risk factors analysis
Authors: Zhou Kai , He Zhongyuan , Tang Ke , Quan Zhengxue 
Published: January 2020

Abstract
Background: The postoperative wound hematoma after anterior cervical spinal surgery is a very critical complication. We aim to explore the risk factors independently associated with postoperative wound hematoma in patients who have undergone anterior cervical spine surgery. Methods: Clinical data of patients with cervical spondylosis or cervical disc herniation who underwent anterior cervical spine surgery by the senior author from January 2011 to December 2017 were evaluated. A multivariate logistic regression was conducted to compare the hematoma group and the non-hematoma group in order to find out which factors that were independently associated with hematoma formation. Mann-Whitney U test was conducted to compare the Neck disability Index score in two groups. Results: A total of 678 patients met the criteria who underwent anterior cervical spine surgery. 13 patients were identified as wound hematoma who have undergone hematoma evacuation, in which hemorrhage sites were found. Multivariate logistic regression identified that history of hypertension (p=0.039 OR=4.42 95%CI 1.08-18.07) and therapeutic heparin use (p=0.020 OR=4.58 95%CI 1.27-16.59) were independent risk factors of hematoma formation. The t-test shows no significant differences between the hematoma group and no hematoma group in APTT and PT levels (p>0.05). Mann-Whitney U test indicates that there was no difference in NDI scores between the two groups(p>0.05). Conclusion: History of hypertension and therapeutic heparin use are risk factors for hematoma formation. Meticulous hemostasis, moderate muscle subtraction, and perioperative airway management are critical in avoiding hematoma development. Hematoma evacuation as quickly as possible is necessary. Key word: wound hematoma, logistic models, risk factors, hypertension, heparin use, hemostasis.
Reverse reaming distraction for acetabular reconstruction of chronic pelvic discontinuity
Authors: Sun Jing-yang , Ni Ming , Ma Hai-yang , Du Yin-qiao , Shen Jun-min , Chen Ji-ying , Zhou Yong-gang , Zhang Guo-qiang 
Published: January 2020

Abstract
Introduction The acetabular distraction technique demonstrates encouraging radiographic and clinical outcomes in treating chronic pelvic discontinuity. The aim of this study is to describe a modified distraction technique and to show our results. Methods This study identified 12 cases of chronic pelvic discontinuity undergoing primary or revision total hip arthroplasty (THA) with the technique of reverse reaming distraction between July 2015 and November 2018. All 12 patients had a minimum followup of 12 months. Radiographs were reviewed to inspect for component loosening. Clinical assessment included the Harris hip score (HHS) and an ambulatory scoring system. Results At the time of final follow-up, no patient was revised. One patient had up to 1 cm migration of the cup in a horizontal or vertical direction and more than 20° change in the abduction angle but was asymptomatic. In the remaining 11 patients, no migration of the component was detected. Both the HHS and ambulatory score showed improvement in all patients. There were no perioperative complications. No postoperative dislocation occured. Conclusions Reverse reaming distraction is a feasible technique in treatment of chronic pelvic discontinuity. Sometimes it can work with the aid of augment or cup-cage construct. When both the superior and inferior portions of the pelvis achieve osteointegration into the porous metal cup, a long-term durability can be expected.
Association between neoangiogenesis after first and second indirect bypass in moyamoya disease
Authors: Ge Peicong , Zhang Qian , Ye Xun , Liu Xingju , Deng Xiaofeng , Wang Jia , Wang Rong , Zhang Yan , Zhang Dong , Zhao Jizong 
Published: January 2020

Abstract
Background The research on neoangiogenesis after indirect bypass for moyamoya disease (MMD) evaluated by using digital subtraction angiography (DSA) is limited. Our study objective was to investigate association between neoangiogenesis after first indirect bypass and second indirect bypass in MMD.Methods All consecutive inpatients with MMD who received indirect bypass at Beijing Tiantan Hospital, Capital Medical University from January 2011 through December 2017 were screened. Bilateral neoangiogenesis was evaluated on lateral views and anteroposterior views by using DSA .Results Twenty-two patients (44 hemispheres) were included in this study. After a median 7.5 months DSA follow-up, On lateral views, 9 (40.9%) hemispheres had grade A, 8 (36.4%) hemispheres had grade B, and 5 (22.7%) hemispheres had grade C after the first procedures; 11 (50.0%) hemispheres had grade A, 7 (31.8%) hemispheres had grade B, and 4 (18.2%) hemispheres had grade C after the second surgery. On anteroposterior views of ECA, 2 (9.1%) hemispheres had level 0, 3 (13.6%) had level 1, 6 (27.3%) had level 2, and 11 (50.0%) had level 3 after the first procedures; 2 (9.1%) hemispheres had level 0, 2 (9.1%) had level 1, 6 (27.3%) had level 2, and 12 (54.6%) had level 3 after second operation. Neovascularization after second operation was strongly associated with the neovascularization after first operation on lateral views (r s =0.770; p=0.000) and the anteroposterior views (r s =0.548; p=0.008).Conclusion Neovascularization after second indirect bypass was strongly associated with neovascularization of first indirect bypass.
The TAND checklist: a useful screening tool in children with tuberous sclerosis and neurofibromatosis type 1
Authors: Cervi Francesca , Saletti Veronica , Turner Katherine , Peron Angela , Bulgheroni Sara , Taddei Matilde , Briola Francesca La , Canevini Maria Paola , Vignoli Aglaia 
Published: January 2020

Abstract

Background: Tuberous Sclerosis Complex (TSC) and Neurofibromatosis type 1 (NF1) are neurocutaneous disorders commonly characterized by neuropsychiatric comorbidities. The TAND (Tuberous Sclerosis Associated Neuropsychiatric Disorders) Checklist is currently used to quickly screen for behavioural, psychiatric, intellectual, academic, neuropsychological and psychosocial manifestations in patients with TSC. We administered the authorized Italian version of the TAND Checklist to 42 TSC patients and to 42 age- and sex-matched NF1 patients, for a total of 84 individuals, aged 4-20 years. Aims of this study: - to test the overall usability of the TAND Checklist in NF1, -to compare the results between children and adolescents with TSC and NF1 and –to assess whether neuropsychiatric manifestations change in relation to the severity of their phenotype. Results: TSC cohort: 35.6% had Intellectual Disability (ID), 11.9% Specific Learning Disorders (SLD), 50.0% Attention Deficit Hyperactivity Disorder (ADHD) and 16.6% anxious/mood disorder. 33.3% had formal diagnosis of Autistic Spectrum Disorder (ASD). Paying attention and concentrating (61.9%), impulsivity (54.8%), temper tantrums (54.8%), anxiety (45.2%), overactivity/hyperactivity (40.5%), aggressive outburst (40.5%), absent or delayed onset of language (40.5%), repetitive behaviors (35.7%), academic difficulties (> 40%), deficits in attention (61.9%) and executive skills (50.0%) were the most commonly reported problems. NF1 cohort: 9.5% had ID, 21.4% SLD, 46.6% ADHD, and 33.3% anxious/mood disorder. No one had a diagnosis of ASD. Commonly reported issues were paying attention and concentrating (59.5%), impulsivity (52.4%), anxiety (50.0%), overactivity/hyperactivity (38.1%), temper tantrums (38.1%), academic difficulties (> 40%), deficits in attention (59.5%), and executive skills (38.1%). Neuropsychiatric features in TSC vs NF1: Aggressive outburst and ASD features were reported significantly more frequently in TSC than in NF1. Conclusion: The TAND Checklist used for TSC is acceptable and feasible to complete in a clinical setting, and is able to detect the complexity of neuropsychiatric involvement in NF1 as well. NF1 is mainly characterized by an ADHD profile, anxiety problems and SLD, while ASD features are strongly associated with TSC. In conclusion, the TAND-Checklist is a useful and reliable screening tool, in both TSC and NF1.
Antimicrobial Susceptibility Profile of Extended Spectrum Beta- lactamases Producing Enterobacteriaceae isolated from clinical samples refereed to the National Bacteriology and Mycology Reference Laboratory, Ethiopia
Authors: Negeri Abebe Aseffa , Seyoum Eyasu Tigabu , Taklu Dejenie Shiferaw , Tsige Estifanos , Assefa Dawit , Ayenew Zeleke , Abdeta Abera , Beyene Degefu , Ebrahim Semira , Addis Tesfa , Mekonnen Yonas , Yitagesu Yohannis , Seyoum Elias , Adamu Etsehiwot , Fentaw Surafel 
Published: January 2020

AbstractBackground Extended-spectrum beta-lactamases (ESBL) producing Enterobacteriaceae are prevalent worldwide and they are unique challenges for treatment and control of bacterial infectious diseases. ESBL genes not only confer resistance to oximino-cephalosporins and aztreonum but also, they are multidrug-resistant to other commonly available antimicrobial agents used in clinical practice.Objective To determine the prevalence and antimicrobial susceptibility profile of ESBL producing Enterobacteriaceae isolated from clinical samples referred to the national clinical bacteriology and mycology reference laboratory.Materials and Methods A cross-sectional study was conducted on Enterobacteriaceae culture- positive clinical samples that were referred to the national bacteriology and mycology reference laboratory from August 2018 to July 2019. Bacterial isolation was performed according to the inoculation and incubation conditions of each clinical specimen and identifications of the isolates were performed using standardized biochemical tests for gram-negative bacteria. Antimicrobial susceptibility profiles of these cultures were determined using the disk diffusion method on Muller Hinton agar according to the recommendation by Clinical and Laboratory Standard Institute (CLSI). ESBL production was detected using CLSI Screening and confirmation test. A double-disk synergy test was used for confirmation.Results Out of 371 culture positive for Enterobacteriaceae , 240 (64.7%) were positive for ESBL production, and the most prevalent species were Klebsiella sp 131(54.6%) followed by E. coli 79 (32.9%). Of 131 ESBL positive Klebsiella spp, 95 (72.5%) were obtained from blood samples and among 79 E. coli isolates, 51 (64.6%) of the strains were isolated from urine samples. All ESBL positive isolates were resistant to ampicillin and all generation of cephalosporins. In addition, 100% of them were multidrug resistant. There were also high proportions of resistant ESBL positive isolates to other classes of antimicrobial agents. Less resistance rates were documented for carbapenems drugs and amikacin from the class of aminoglycosides.Conclusion ESBL producing Enterobacteriaceae we reported in this study was not only highly prevalent but also they are multidrug resistant to most clinically available antimicrobial agents including carbapenems. Therefore, public awareness and regular monitoring
Dissection of the mutation accumulation process during bacterial range expansions
Authors: Bosshard Lars , Peischl Stephan , Ackermann Martin , Excoffier Laurent 
Published: January 2020

Abstract
Background Recent experimental work has shown that the evolutionary dynamics of bacteria expanding across space can differ dramatically from what we expect under well-mixed conditions. During spatial expansion, deleterious mutations can accumulate due to inefficient selection on the expansion front, potentially interfering with and modifying adaptive evolutionary processes.Results We used whole genome sequencing to follow the genomic evolution of 10 mutator Escherichia coli lines during 39 days (∼1650 generations) of a spatial expansion, which allowed us to gain a temporal perspective on the interaction of adaptive and non-adaptive evolutionary processes during range expansions. We used elastic net regression to infer the positive or negative effects of mutations on colony growth. Even though the colony size, measured after three day of growth, decreased at the end of the experiment in all 10 lines, and mutations accumulated at a nearly constant rate over the whole experiment. We find evidence that beneficial mutations accumulate primarily at an early stage of the experiment, leading to a non-linear change of colony size over time. Indeed, colony size remains almost constant at the beginning of the experiment and then decreases after ∼12 days of evolution. We also find that beneficial mutations are enriched in flagella genes, genes encoding transport proteins, and genes coding for the membrane structure, whereas deleterious mutations show no enrichment for any biological process.Conclusions Our experiment shows that beneficial mutations target specific biological functions mostly involved in inter or extra membrane processes, whereas deleterious mutations are randomly distributed over the whole gnome. It thus appears that the interaction between genetic drift and the availability or depletion of beneficial mutations determines the change in fitness of bacterial populations during range expansion.
The effects of intermittent fasting on liver physiology and metabolism in mice
Authors: Ma Jianbo , Cheng Yan , Su Qiang , Ai Wen , Gong Ling , Wang Yueying , Li Linhao , Ma Zhongren , Pan Qiuwei , Qiao Zilin , Chen Kan 
Published: January 2020

Abstract
Background: Intermittent fasting, as an emerging diet concept, has been widely practiced in the global population. A broad spectrum of health benefits has been reported in animal models and in humans for intermittent fasting. However, the underlying mechanisms remain largely elusive. In this study, we aimed to explore the effects and potential mode-of-action of intermittent fasting in mouse models, with focus on the liver.Methods: C57BL/6 mice were divided into five groups of 8-14 each, including 30-days ad libitum group, 30-days intermittent fasting group, 60-days ad libitum group, 60-days intermittent fasting group and refeeding group (30-days intermittent fasting followed by 30-days ad libitum). The food intake, body weight, liver weight and the blood biochemical parameters were detected. Targeted metabolic profiling of liver was performed.Results: We found that daily 12-hour intermittent fasting for one or two months significantly reduced the cumulative food intake, compared with the mice fed ad libitum. Fasting resulted in significantly reduced liver weight, with minimal effect on body weight. This effect on the liver by one month fasting could not be reversed by following one-month ad libitum feeding. Among the measured blood biochemical parameters, glucose level was decreased, while alkaline phosphatase was increased in the fasting mice. Surprisingly, targeted metabolic profiling revealed the global elevation of metabolites in the livers of fasting mice. These metabolic molecules include ATP, NADP, NADPH and succinate that are essentially involved in the citric acid cycle and oxidative phosphorylation. Conclusion: Daily 12-hour intermittent fasting for one to two months significantly reduced liver weight in mice, which is associated with enhanced liver metabolism.
Sedentary behavior, physical activity and renal function in middle-aged and older adults: isotemporal substitution modelling
Authors: Kosaki Keisei , Tanahashi Koichiro , Matsui Masahiro , Akazawa Nobuhiko , Osuka Yosuke , Tanaka Kiyoji , Dunstan David W. , Owen Neville , Shibata Ai , Oka Koichiro , Maeda Seiji 
Published: January 2020

Abstract
Background: Both physical inactivity and sedentary behavior (too much sitting) can contribute to renal dysfunction. However, the potential benefits of behavior change (e.g., replacing sedentary behavior with physical activity) on renal function are not fully understood. We used isotemporal substitution to model potential impacts on renal function of replacing time from one behavior to another in middle-aged and older adults.
Methods: For 174 Japanese adults (aged 50 – 83 years; 76 % women), the time spent in sedentary behavior, light-intensity physical activity (LPA), and moderate to vigorous-intensity physical activity (MVPA) were assessed using uniaxial accelerometers. Renal function was evaluated by the estimated glomerular filtration rate (eGFR) from serum creatinine and cystatin C levels.
Results: In univariate analyses, eGFR was significantly, albeit weakly, correlated with time spent in sedentary behavior ( r s = – 0.229), LPA ( r s = 0.265) and with MVPA ( r s = 0.353). In the isotemporal substitution models, replacement of 30 min/day of sedentary behavior with equivalent LPA time was not significantly associated with eGFR ( β = 2.25, p = 0.111); however, replacement with the same amount of MVPA was beneficially associated with eGFR ( β = 5.51, p < 0.05).
Conclusions: These cross-sectional findings suggest that both sedentary behavior (detrimentally) and physical activity (beneficially) may be contributors to maintaining renal function and that replacing sedentary behavior with MVPA may benefit renal health in middle-aged and older adults.
Identification of key genes of papillary thyroid carcinoma by integrated bioinformatics analysis
Authors: Xue Gang , Lin Xu , Wu Jingfang , Pei Da , Wang Dong-Mei , Zhang Jing , Zhang Wen-Jing 
Published: January 2020

Abstract
Background:Papillary thyroid carcinoma (PTC) is one of the fastest-growing malignant tumor types of thyroid cancer. Therefore, identifying the interaction of genes in PTC is crucial for elucidating its pathogenesis and finding more specific molecular biomarkers. Methods:In this study, 4 pairs of PTC tissues and adjacent tissues were sequenced using RNA-Seq, and 3745 differentially expressed genes (DEGs) were screened. The results of GO and KEGG enrichment analysis indicate that the vast majority of DEGs may play a positive role in the development of cancer. Then, the significant modules were analysed using Cytoscape software in the protein-protein interaction (PPI) network. Survival analysis, TNM analysis, and immune infiltration analysis of key genes are all analyzed. And the expression of ADORA1, APOE and LPAR5 genes was verified by qPCR in papillary thyroid carcinoma compared to their matching adjacent tissues.Results: A total of 25 genes were identified as hub genes with nodes greater than 10. The expression of 25 key genes in PTC were verified by the GEPIA database, and the overall survival and disease free survival analyses of these key genes were conducted with Kaplan–Meier plots. We found that only three genes were confirmed with our validation and were statistically significant in PTC, namely ADORA1, APOE, and LPAR5. Further analysis found that the mRNA levels and methylation degree of these three genes are significantly correlated with the TNM staging of PTC, and these three genes are related to PTC immune infiltration. Verification of the expression of these three genes by RT-qPCR further confirmed the reliability of our results. Conclusion: Our study identified three genes that may play key regulatory roles in the development, metastasis, and immune infiltration of papillary thyroid carcinoma.Key words :RNA-Seq, papillary thyroid carcinoma, key gene, bioinformatics
The new K, Pb-bearing uranyl-oxide mineral kroupaite: Crystal-chemical implications for the structures of uranyl-oxide hydroxy-hydrates
Authors: Plášil Jakub , Kampf Anthony R. , Olds Travis A. , Sejkora Jiří , Škoda Radek , Burns Peter C. , Čejka Jiří 
Published: January 2020

AbstractKroupaite (IMA 2017-031), ideally KPb0.5[(UO2)8O4(OH)10]·10H2O, is a new uranyl-oxide hydroxylhydrate mineral found underground in the Svornost mine, Jáchymov, Czechia. Electron-probe micro-analysis (WDS) provided the empirical formula (K1.28Na0.07)Σ1.35(Pb0.23Cu0.14Ca0.05Bi0.03Co0.02Al0.01)Σ0.48 [(UO2)7.90(SO4)0.04O4.04(OH)10.00]·10H2O, on the basis of 40 O atoms apfu. Sheets in the crystal structure of kroupaite adopt the fourmarierite anion topology, and therefore kroupaite belongs to the schoepite-family of minerals with related structures differing in the interlayer composition and arrangement, and charge of the sheets. Uptake of dangerous radionuclides (90Sr or 135Cs) into the structure of kroupaite and other uranyl-oxide hydroxy-hydrate is evaluated based on crystal-chemical considerations and Voronoi-Dirichlet polyhedra measures. These calculations show the importance of these phases for the safe disposal of nuclear waste.
New insights into the nature of glauconite
Authors: López-Quirós Adrián , Sánchez-Navas Antonio , Nieto Fernando , Escutia Carlota 
Published: January 2020

AbstractGlauconite must be assessed as mica-rich mica-smectite R3 interstratified mineral, with the pure end-member mica also having intrinsic K-deficient chemical characteristics (K+ ~ 0.8 apfu). This assertion is in accordance with our X-ray diffraction (XRD) and high-resolution tranmission electron microscopy (HRTEM) studies and chemical analyses by electron probe microanalysis (EPMA) of mature glauconites in Cenozoic Antarctic sediments that indicate that: (1) It consists of a glauconite-smectite (R3 ordered) mixed-layer silicate, composed mainly of mica-type layers (&gt;90%), but displaying slightly different proportions of Fe(III)-smectite layers (&lt;10%). (2) More mature glaucony grains are characterized by major K+ and VIFe2+ (mica layers) and minor VIFe3+ (smectite layers) content in the interstratified glauconite-smectite. (3) Potassium is stabilized at the interlayer site by the octahedrally coordinated Fe2+. (4) Microtexture of the glauconite crystals are comparable with those of other micas and illite minerals, with straight, defect-free lattice fringes of ~10 Å spacings glauconite packets characteristic of mica with minor interstratified poorly crystalline smectite layers. In addition, our new findings give insights into the glauconitization process and at the same time investigate the potassium-deficient character of the dioctahedral mica “glauconite.” These findings show that glauconite crystallizes by a layer-growth mechanism at the expense of a poorly crystalline smectite precursor and that smectiteto-glauconite transformations are accompanied by a gradually higher octahedral charge deficiency (Fe2+/Fe3+) stabilized by K+ uptake into the interlayer sheet.
Role of Corporate Governance in the Voluntary Disclosure of Intellectual Capital
Authors: Kamat G Bharathi 
Published: January 2020

The research on intangible assets and its contribution to the value creation of the firms has received significant attention in recent times. This paper attempts to estimate and analyse the nature and extent of overall Intellectual Capital Disclosure (ICD) and its sub-components in the annual reports of Information Technology (IT) industry in India for the FY 2017-18. It also attempts to examine whether Corporate Governance Characteristics of these firms influence their ICD. Multiple linear regression analysis is used to evaluate the data on information technology Industry. IT firms in India disclose a very high extent of Human capital; whereas the nature of disclosure is high in case of Structural Capital. The empirical evidence suggests that board size, its independence and ownership pattern of the firm play a significant role in the extent of overall intellectual capital disclosures in Indian IT firms. The result on impact of corporate governance characteristics varies with respect to the IC sub-components.