Depistage De Lâ€™anemie En Urgence Au Chu Campus : Validation Du Degre De Concordance Entre Les Resultats De Lâ€™hemocueÂ® Hb 301 Et Ceux Du Sysmex Xn-1000Â®
Authors: Codjo. Kouassi Kafui Codjo. Kouassi , A .H. N Tettekpoe Micheline , Katawa Gnatoulma , Hounogbe Agboka , S. Karou Damintoti 
Published: December 2020

Introduction: Un dÃ©pistage fiable de lâ€™anÃ©miÃ© dans un contexte dâ€™urgence grÃ¢ce Ã un hÃ©moglobinomÃ¨tre de biologie dÃ©localisÃ©e demeure un dÃ©fis en Afrique. Le but de cette Ã©tude Ã©tait dâ€™Ã©valuer le degrÃ© de concordance des rÃ©sultats dâ€™hÃ©moglobinÃ©mie obtenus sur lâ€™HemoCueÂ® Hb 301 par rapport Ã ceux de lâ€™analyseur Sysmex XN-1000Â®. MÃ©thodes : Il sâ€™est agi dâ€™une Ã©tude rÃ©trospective et transversale descriptive rÃ©alisÃ©e du 08 Mai 2017 au 23 FÃ©vrier 2018 au CHU Campus du Togo. La performance du Sysmex XN-1000Â® a Ã©tÃ© confirmÃ©e grÃ¢ce Ã lâ€™Ã©valuation de lâ€™exactitude et de la fidÃ©litÃ© de mesure. La performance de lâ€™HemocueÂ® Hb 301 a Ã©tÃ© Ã©valuÃ©e au moyen dâ€™une rÃ©gression linÃ©aire et du diagramme de Bland et Altman vis-Ã -vis du Sysmex XN-1000Â®. Les critÃ¨res de performances utilisÃ©s Ã©taient ceux du Clinical Laboratory Improvement Amendments (CLIA) et de RICOS et al. RÃ©sultats: Le niveau dâ€™exactitude selon CLIA du Sysmex XN-1000Â® Ã©tait de 100%. Lâ€™Ã©quation de la droite de rÃ©gression Ã©tait: Y (HemoCueÂ® Hb 301) = 0,99x (Symex XN-1000Â®) + 0,54 g/dl. Les valeurs mÃ©dianes des 100 Ã©chantillons sur le Sysmex et lâ€™Hemocue Ã©taient respectivement de 12,55 g/dl et 13,00 g/dl avec un biais mÃ©dian de 3,58%, qui Ã©tait infÃ©rieur au biais limite de Â±7,0% (CLIA). Conclusion: Le Sysmex XN-1000Â® fournit des rÃ©sultats dâ€™hÃ©moglobinÃ©mie interchangeables avec ceux de lâ€™HemoCueÂ® Hb 301, sans altÃ©ration de la capacitÃ© diagnostique pour le patient. Il reste donc performant pour un quelconque dosage dâ€™hÃ©moglobinÃ©mie en urgence.
Introduction: Reliable anemia screening in an emergency setting, using a Point-of-care hemoglobinometer remains a challenge in Africa. The purpose of this study was to assess the degree of agreement between hemoglobin results obtained on the HemoCueÂ® Hb 301 compared to those of the Sysmex XN-1000Â® Analyzer. Methods: This was a retrospective and cross-sectional descriptive study carried out from May 08 2017 to February 23, 2018 at the Campus teaching hospital of Togo. The performance of the Sysmex XN-1000Â® was confirmed by evaluating the measurement accuracy and precision. The performance of HemocueÂ® Hb 301 was assessed using linear regression and a Bland and Altman plot against Sysmex XN-1000Â®. The performance criteria used were those of Clinical Laboratory Improvement Amendments (CLIA) and RICOS et al. Results: The CLIA level of accuracy of the Sysmex XN-1000Â® was 100%. The equation for the regression line was: Y (HemoCueÂ® Hb 301) = 0.99x (Symex XN-1000Â®) + 0.54 g / dl. The median values of the 100 samples on the Sysmex and Hemocue were 12.55 g / dl and 13.00 g / dl respectively, with a median bias of 3.58%, which was less than the borderline bias of Â± 7.0 % of CLIA. Conclusion: The Sysmex XN-1000Â® provides interchangeable hemoglobin results with those of HemoCueÂ® Hb 301, without altering the diagnostic ability for the patient. It therefore remains effective for any emergency dosage of hemoglobinemia.