bluuebunny/crossref_metadata_2025_split huggingface dataset:
RAF conformational autoinhibition and 14-3-3 proteins promote paradoxical activation
Authors: Mendiratta Gaurav , Abbott Kodye , Li Yao-Cheng , Yu Jingting , Huang Jianfeng , Shokhirev Maxim N. , McFall Thomas , Wahl Geoffrey M. , Stites Edward C. 
Published: November 2019

AbstractRAF kinase inhibitors can, in some conditions, increase RAF kinase signaling. This process, which is commonly referred to as “paradoxical activation” (PA), is incompletely understood. RAF kinases are regulated by autoinhibitory conformational changes, and the role of these conformational changes in PA is unclear. Our mathematical investigations reveal that a dynamic equilibrium between autoinhibited and non-autoinhibited forms of RAF, along with the RAF inhibitor stabilization of the non-autoinhibited form, can be sufficient to create PA. Using both computational and experimental methods we demonstrate that 14-3-3 proteins, which stabilize both RAF autoinhibition and RAF dimerization, potentiate PA. Our model led us to hypothesize that increased 14-3-3 expression would amplify PA for the third generation RAF inhibitors that normally display minimal to no PA. Our subsequent experiments find that 14-3-3 overexpression increases PA, increases RAF dimerization, and promotes resistance to these inhibitors, effectively “breaking” these “paradox breaker” and pan-RAF inhibitors. Overall, this work reveals a robust mechanism for PA based solely on equilibrium dynamics of canonical interactions in RAF signaling and identifies conditions which allow PA to occur.
A latent profile analysis of work passion: structure, antecedent, and outcomes
Authors: Li Jingjing , Zhang Jian , Shao Bo , Chen Chunxiao 
Published: November 2019

Purpose
Previous research draws on the dualistic model of passion (harmonious and obsessive passion) overlooks how the different two types of passion interact within individuals using a variable-centered approach. The purpose of this paper is to identify work passion profiles and their antecedent and consequences adopting a person-centered approach, and to explain inconsistences in previous studies.

Design/methodology/approach
This paper conducts three studies (n=2,749 in total) using a latent profile analysis. Study 1 identifies three work passion profiles, namely, dual passion, pro harmonious passion and pro obsessive passion; study 2 examines dialectical thinking as an antecedent to work passion profile membership; study 3 examines how each profile relates to work performance and well-being.

Findings
This paper finds that the participants with a dual passion profile showed higher task performance and subjective well-being than the participants with the other two profiles; the participants with a pro obsessive passion profile were higher in task performance, interpersonal performance and psychological well-being than the participants with a pro harmonious profile.

Originality/value
This paper is the first that uses a latent profile analysis approach to examining work passion configurations. It provides a unique perspective to investigate how different types of passion configure and interact within individuals; it explores an antecedent (i.e. dialectical thinking) and outcomes (i.e. performance and well-being) of the three work passion profiles.
HRM reforms and job-related well-being of academics
Authors: Xia Jie , Zhang Mingqiong Mike , Zhu Jiuhua Cherrie , Fan Di , Samaratunge Ramanie 
Published: November 2019

PurposeThe purpose of this paper is to examine the influence of human resource management (HRM) reforms on job-related well-being of academics in Chinese universities. It also tests the mediating effect of work intensification (WI) and affective commitment (AC), and the moderating effect of perceived organizational justice (OJ) on the HRM‒well-being relationship to understand the influence mechanisms and boundary conditions.Design/methodology/approachA questionnaire survey was conducted in 25 Chinese universities, obtaining 638 usable questionnaires. Structural equation modeling (SEM) was used as the analytical technique to examine the model fit and test hypotheses.FindingsThe findings reveal that the relationship of HRM and well-being is neither direct nor unconditional, and a win‒win scenario for both management and employee well-being is possible when organizations pursue HRM innovations.Research limitations/implicationsThe limitations of this study are that data were collected at once and at a defined time, with no time lag being involved. In addition, all variables were self-reported.Practical implicationsCommitment-oriented HRM practices can create a win‒win scenario; when control-oriented HRM practices are necessary, managers should ensure OJ to offset their negative influence on employees.Originality/valueThis study is among the first to examine the impact of HRM on employee well-being in the context of Chinese higher education, contributing to the limited studies on HRM in Chinese public sector and the on-going debate on the nature of HRM in China.
Human influences on antipredator behaviour in Darwin’s finches
Authors: Gotanda Kiyoko M. 
Published: November 2019

Abstract

Humans exert dramatic influences upon the environment, creating novel selective pressures to which organisms must adapt. On the Galapagos, humans have established a permanent presence and have altered selective pressures through influences such as invasive predators and urbanization, affecting iconic species such as Darwin's finches.

Here, I ask two key questions: (a) Does antipredator behaviour (e.g., flight initiation distance – FID) change depending on whether invasive predators are historically absent, present, or eradicated? and (b) To what degree does urbanization affect antipredator behaviour? This study is one of the first to quantify antipredator behaviour in endemic species after the eradication of invasive predators. This will help to understand the consequences of invasive predator eradication and inform conservation measures.

I quantified FID, an antipredator behaviour, in the small ground finch, across multiple islands in the Galapagos that varied in the presence, absence, or successful eradication of invasive predators. On islands with human populations, I quantified FID in urban and non‐urban populations of finches.

FID was higher on islands with invasive predators compared to islands with no predators. On islands from which invasive predators were eradicated ~11 years previously, FID was also higher than on islands with no invasive predators. Within islands that had both urban and non‐urban populations of finches, FID was lower in urban finch populations, but only above a threshold human population size. FID in larger urban areas on islands with invasive predators was similar to or lower than FID on islands with no history of invasive predators.

Overall, these results suggest that invasive predators can have a lasting effect on antipredator behaviour, even after eradication. Furthermore, the effect of urbanization can strongly oppose the effect of invasive predators, reducing antipredator behaviour to levels lower than found on pristine islands with no human influences. These results improve our understanding of human influences on antipredator behaviour which can help inform future conservation and management efforts on islands.
Harpellales (Kickxellomycotina: Zygomycetes) fungi associated with the digestive tract of larval black flies (Diptera: Simuliidae) in Korea
Authors: Kim Sam‐kyu 
Published: November 2019

AbstractHarpellales fungi associated with larval black flies were studied for the first time in Korea. They are a common, widespread group of filamentous fungi that, as obligate symbionts, inhabit the digestive tracts of many terrestrial and aquatic arthropods. Total of 842 ultimate/penultimate instar larvae of black flies from 53 stream were dissected to detect trichomycetes colonization. As a result of the study, seven species of Harpellales including single undetermined Pennella species was found from eight species of black fly hosts: Harpella melusinae, Pennella angustispora, Pennella hovassi, Pennella KNU‐1, Simuliomyces microsporus, Smittium simulii, and Smittium megazygosporum. Descriptions for general features including size and shape of trichospore and zygospores (if available) of each species and their host larval black flies were provided. A key to all known Harpellales fungi found in larval black flies from Korea was also provided.
Flood Risk Reduction from Agricultural Best Management Practices
Authors: Antolini Federico , Tate Eric , Dalzell Brent , Young Nathan , Johnson Kris , Hawthorne Peter L. 
Published: November 2019

AbstractBest management practices (BMPs) play an important role in improving impaired water quality from conventional row crop agriculture. In addition to reducing nutrient and sediment loads, BMPs such as fertilizer management, reduced tillage, and cover crops could alter the hydrology of agricultural systems and reduce surface water runoff. While attention is devoted to the water quality benefits of BMPs, the potential co‐benefits of flood loss reduction are often overlooked. This study quantifies the effects of selected commonly applied BMPs on expected flood loss to agricultural and urban areas in four Iowa watersheds. The analysis combines a watershed hydrologic model, hydraulic model outputs, and a loss estimation model to determine relationships between hydrologic changes from BMP implementations and annual economic flood loss. The results indicate a modest reduction in peak discharge and economic loss, although loss reduction is substantial when urban centers or other high‐value assets are located downstream in the watershed. Among the BMPs, wetlands, and cover crops reduce losses the most. The research demonstrates that watershed‐scale implementation of agricultural BMPs could provide benefits of flood loss reduction in addition to water quality improvements.
The association of husbands’ smoking with wives' dysglycemia status: A cross‐sectional study among over 10 million Chinese women aged 20‐49
Authors: Zhang Minjin , Yang Ying , Liu Fangchao , Jia Jiajing , Xu Qin , Wang Long , Wang Yuanyuan , He Yuan , Zhang Ya , Peng Zuoqi , Wang Qiaomei , Shen Haiping , Xu Zongyu , Zhang Yiping , Yan Donghai , Zhang Hongguang , Ma Xu 
Published: November 2019

AbstractBackgroundWe comprehensively evaluated the association between husbands’ smoking amount and wives' dysglycemia status.MethodsWe conducted a cross‐sectional study which recruited 12 023 714 reproductive couples who participated in the National Free Pre‐Pregnancy Checkups Project (NFPCP) between 2015 and 2016 in China. Multivariable logistic models were used to estimate odds ratio (OR) and 95% CI. Subgroup analysis and stratified analysis were further performed to investigate potential effect modification.ResultsCompared to the neither‐smoker group, the multivariable‐adjusted ORs for diabetes mellitus (DM) among women was 1.09 (95% CI, 1.07‐1.10), 1.04 (95% CI, 0.87‐1.23), and 1.28 (95% CI, 1.17‐1.41) in the husband‐smoker, wife‐smoker, and both‐smokers groups, respectively. An increased risk of DM was also observed for women whose husbands smoke 1 to 10 (OR, 1.06; 95% CI, 1.04‐1.07), 11 to 20 (OR, 1.13; 95% CI, 1.11‐1.15), and over 20 cigarettes per day (OR, 1.25; 95% CI, 1.17‐1.31). The prevalence of wives' DM showed significant linear trends with husbands’ smoking amount (Pfor trend < .001). A similar growth tendency was also observed between husband smoking amount and impaired fasting glucose (IFG) prevalence with ORs of 1.04 (95% CI, 1.04‐1.05), 1.05 (95% CI, 1.04‐1.06), and 1.09 (95% CI, 1.06‐1.11) for 1‐10, 11‐20, and over 20 cigarettes per day, respectively. The relationship between husbands’ smoking amount and wives’ IFG/DM prevalence appear to be modified by body mass index (BMI).ConclusionsThis study shows significant relationships between husbands’ smoking and wives' risk of IFG/DM, and this result exists in both a categorical and dose‐response manner. This association is modified by BMI. Family‐oriented smoking interventions may both reduce husbands’ active smoking and wives’ risk of DM.
Subcortical surface morphometry in substance dependence: An ENIGMA addiction working group study
Authors: Chye Yann , Mackey Scott , Gutman Boris A. , Ching Christopher R.K. , Batalla Albert , Blaine Sara , Brooks Samantha , Caparelli Elisabeth C. , Cousijn Janna , Dagher Alain , Foxe John J. , Goudriaan Anna E. , Hester Robert , Hutchison Kent , Jahanshad Neda , Kaag Anne M. , Korucuoglu Ozlem , Li Chiang‐Shan R. , London Edythe D. , Lorenzetti Valentina , Luijten Maartje , Martin‐Santos Rocio , Meda Shashwath A. , Momenan Reza , Morales Angelica , Orr Catherine , Paulus Martin P. , Pearlson Godfrey , Reneman Liesbeth , Schmaal Lianne , Sinha Rajita , Solowij Nadia , Stein Dan J. , Stein Elliot A. , Tang Deborah , Uhlmann Anne , van Holst Ruth , Veltman Dick J. , Verdejo‐Garcia Antonio , Wiers Reinout W. , Yücel Murat , Thompson Paul M. , Conrod Patricia , Garavan Hugh 
Published: November 2019

AbstractWhile imaging studies have demonstrated volumetric differences in subcortical structures associated with dependence on various abused substances, findings to date have not been wholly consistent. Moreover, most studies have not compared brain morphology across those dependent on different substances of abuse to identify substance‐specific and substance‐general dependence effects. By pooling large multinational datasets from 33 imaging sites, this study examined subcortical surface morphology in 1628 nondependent controls and 2277 individuals with dependence on alcohol, nicotine, cocaine, methamphetamine, and/or cannabis. Subcortical structures were defined by FreeSurfer segmentation and converted to a mesh surface to extract two vertex‐level metrics—the radial distance (RD) of the structure surface from a medial curve and the log of the Jacobian determinant (JD)—that, respectively, describe local thickness and surface area dilation/contraction. Mega‐analyses were performed on measures of RD and JD to test for the main effect of substance dependence, controlling for age, sex, intracranial volume, and imaging site. Widespread differences between dependent users and nondependent controls were found across subcortical structures, driven primarily by users dependent on alcohol. Alcohol dependence was associated with localized lower RD and JD across most structures, with the strongest effects in the hippocampus, thalamus, putamen, and amygdala. Meanwhile, nicotine use was associated with greater RD and JD relative to nonsmokers in multiple regions, with the strongest effects in the bilateral hippocampus and right nucleus accumbens. By demonstrating subcortical morphological differences unique to alcohol and nicotine use, rather than dependence across all substances, results suggest substance‐specific relationships with subcortical brain structures.
Which adolescent factors predict alcohol misuse in young adulthood? A co‐twin comparisons study
Authors: Stephenson Mallory , Barr Peter , Ksinan Albert , Aliev Fazil , Latvala Antti , Viken Richard , Rose Richard , Kaprio Jaakko , Dick Danielle , Salvatore Jessica E. 
Published: November 2019

AbstractBackground and aimsResearch on adolescent predictors of later alcohol misuse is typically conducted on samples of singletons, and associations may be confounded by between‐family differences. To address potential confounding, we applied a co‐twin comparison design to evaluate whether differences between co‐twins in a wide array of adolescent risk factors predicted differences in young adult alcohol misuse.DesignLongitudinal study in which associations between characteristics of the sample as adolescents were used to predict young adult alcohol misuse in individual‐level analyses and co‐twin comparisons.SettingFinland.ParticipantsA total of 3402 individuals (1435 complete twin pairs; 36% monozygotic; 57% female) from the FinnTwin12 study.MeasurementsThe young adult alcohol misuse outcome was a composite score of alcohol use and intoxication frequency. Adolescent predictors included factor scores representing academic performance, substance use, externalizing problems, internalizing problems, peer environment, physical health and relationship with parents; and single measures tapping alcohol expectancies, life events and pubertal development.FindingsIn individual‐level analyses, individuals with higher adolescent substance use, externalizing problems, time with friends, peer deviance, sports involvement, sleeping difficulties, parental discipline, positive alcohol expectancies and difficulty of life events reported higher alcohol misuse in young adulthood (Ps < 0.019, R2 = 0.0003–0.0310%). Conversely, those with higher adolescent internalizing problems, parent–child relationship quality and time with parents reported lower alcohol misuse (Ps < 0021, R2 = 0.0018–0.0093%). The associations with adolescent substance use and alcohol expectancies remained significant in co‐twin comparisons (Ps < 0.049, R2 = 0.0019–0.0314%). Further, academic performance emerged as a significant predictor, such that individuals with higher grades compared with their co‐twin reported higher young adult alcohol misuse (Ps < 0.029, R2 = 0.0449–0.0533%).ConclusionsAdolescent substance use, positive alcohol expectancies and higher academic performance appear to be robust predictors of later alcohol misuse.
Structural and dynamic analysis of G558R mutation in chicken TSHR gene shows altered signal transduction and corroborates its role as a domestication gene
Authors: Grottesi A. , Gabbianelli F. , Valentini A. , Chillemi G. 
Published: November 2019

SummaryThe thyroid‐stimulating hormone receptor (TSHR) has been indicated as a putative domestication gene in chicken. Comparison of WGS identified a variant in residue 558 of the transmembrane domain (TM) of TSHR, where the domestic chicken (GGD) presents an arginine, whereas the red jungle fowl (RJF) shares a conserved glycine with other vertebrates. This variant has been demonstrated to be associated with phenotypes that are important for domestication and related to thyroid regulation, such as less fearful behavior, reduced aggressive behavior and reduced dependence on seasonal reproduction in GGD as compared with RJF. By means of molecular dynamics simulations, we highlighted the structural and dynamic differences of variant Gly558Arg in the TSHR TM domain. Alterations in TM helix flexibility, structure and protein overall motion are described. The so‐called ‘arginine snorkeling’ of residue 568 in GGD is observed and we hypothesize it as the originating force that produces the observed whole‐protein perturbation in the helix bundle dynamics, capable of altering the TSHR signal transduction. The results are discussed in the context of their implications for a better understanding of biological mechanisms in chicken under control of the thyroid, such as body metabolism, as well as for their usefulness in biomedical research.
Microbiology of pancreatoduodenectomy and recommendations for antimicrobial prophylaxis
Authors: Swan Christopher D. , Nahm Christopher , Samra Jaswinder S. , Mittal Anubhav , Figtree Melanie 
Published: November 2019

AbstractBackgroundThe microbiology of pancreatoduodenectomy is challenging and published guidelines regarding perioperative antimicrobial prophylaxis are variable with poor adherence.MethodsA retrospective analysis of the microbiological results of 294 consecutive patients who underwent pancreatoduodenectomy was performed. Intraoperative specimen culture results were available for 50 patients and their medical records were reviewed to determine the following demographics and factors; age; sex; tumour location, histopathology, grade and stage; neoadjuvant chemotherapy and radiotherapy; preoperative biliary stenting; surgeon; surgery type and antimicrobial prophylaxis coverage. Outcomes assessed included; post‐operative infections, mortality (all and 90‐day), and intensive care unit and hospital admission durations. Univariate analysis with chi‐squared testing was performed.ResultsIntraoperative specimen cultures were positive in 48 (96%) patients and polymicrobial in 45 (90%) patients with a predominance of Enterobacteriaceae (38/76%), Enterococcus species (27/54%), and Candida species (25/50%). Isolates were potentially susceptible to the current perioperative antimicrobial prophylaxis regimen of ceftriaxone with or without metronidazole in only six patients. However, only neoadjuvant radiotherapy was associated with statistically significant increased intensive care unit and hospital admission durations.ConclusionAlthough this study was probably underpowered to detect any statistically significant associations, perioperative antimicrobial prophylaxis coverage of the operative field microbiological milieu of pancreatoduodenectomy is logical and current guidelines may be inadequate.
Successful treatment of a cohort of infants with neonatal diabetes using insulin pumps including data on genetics and estimated incidence
Authors: Torbjörnsdotter Torun , Marosvari‐Barna Elisabeth , Henckel Ewa , Corrias Martino , Norgren Svante , Janson Annika 
Published: November 2019

AbstractAimNeonatal diabetes is rare, and treatment is challenging. We present aspects on treatment, genetics and incidence.MethodThis was a prospective cohort study including all cases in our study area in Sweden. We compared with data from the National Diabetes Registry, the Neonatal Quality Register and the National Patient Register.ResultsIn the 19‐year study period January 1, 1998 to December 31, 2016, we treated seven infants, five of them boys. Six patients used a subcutaneous insulin pump, and the smallest patient started at a weight of 938 g. Most important was for the pump to deliver minute doses of insulin and the design of cannulas and tubing. All patients could stop insulin treatment at 17‐145 days of age. One patient relapsed at age 4.5 years. Four patients used the insulin pump after discharge. A mutation was identified in five patients, and this included all patients born after 30 weeks of gestation. The incidence of neonatal diabetes was 2/1 00 000, higher than previously estimated for Europe. Similar but lower incidences were reported in the registries.ConclusionInsulin pumps were safe in neonatal diabetes. All seven cases were transient. Neonatal diabetes was more common in our area than reported from Europe.
The Effects of Ageing on Creative Performance Trajectories
Authors: Khan Mahreen , Minbashian Amirali 
Published: November 2019

Previous research has demonstrated that job performance initially increases, plateaus, and then eventually declines. A major factor that may be relevant for explaining these performance changes is the ageing process. This study examined the within‐person effect of ageing on changes in a specific type of job performance (creative performance), and whether the age of career onset is a moderator. These questions were investigated using two measures of creative performance data spanning several decades from 300 authors and analysed using hierarchical linear modelling (HLM) techniques and hierarchical generalised linear modelling techniques (HGLM). The results revealed an inverted U‐shaped relationship between time and creative performance, and an inverted U‐shaped relationship between ageing and creative performance when controlling for work experience. The age at which an individual starts their career was a significant moderator of the shape of performance trajectories. Findings are discussed in relation to how this study contributes to the literature on performance trajectories and the effects of ageing on performance.