bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bibliometric analysis and visualization of the research on the relationship between RNA methylation and immune cell infiltration in tumors
Authors: Meng Sibo , Yang Guanghui , Yu Enhao , Li Jiaxin 
Published: December 2024

BackgroundThis research endeavors to delve into the research hotspots and trends concerning RNA methylation and tumor immune cells through the application of bibliometric analysis and visualization techniques.MethodsA comprehensive search in WoSCC (2014-2023) for RNA methylation and tumor immune cell articles/reviews was conducted. Bibliometric analysis and visualization employed CiteSpace, Bibliometric, and VOSviewer tools.ResultsA total of 3295 articles were included in the analysis, with a continuously increasing number of publications linking RNA methylation to tumoral immune cells. Chinese authors and research institutions have demonstrated a sustained growth trend in both the number of publications and author influence. SUN YAT SEN UNIVERSITY, a Chinese institution, has published the highest number of articles in this field, while also demonstrating extensive international and inter-institutional collaborations. Meanwhile, HARVARD UNIVERSITY has also achieved impressive results. For instance, Frontiers in Immunology has published the largest number of articles in this category. Nature Communications has published articles that are most influential in this field, playing a pivotal role in disseminating research findings. The sustained vitality of this field is attributed to its solid research foundation, including the groundbreaking work published by Professor Chiappinelli KB in Cell and the widely cited paper by Professor Han DL in Nature. Analysis of research trend topics reveals that m5C, immunotherapy, and the immune microenvironment are current research focuses.ConclusionFuture investigative efforts at the juncture of RNA methylation and tumor immune cells are anticipated to concentrate on domains including m5C, n7-methylguanosine, cuproptosis, prognosis assessment, immunotherapeutic strategies, and the tumor microenvironment.
Burden and clinical characteristics of recurrent Plasmodium vivax infections, and impact of primaquine for radical cure: a systematic scoping review in India
Authors: Kojom Foko Loick Pradel , Singh Vineeta 
Published: December 2024

BackgroundIndia accounts for the bulk of Plasmodium vivax burden in South East Asia. Primaquine (PQ) is the only currently available drug for treating relapses in P. vivax malaria. MethodsHere, we provide an overview of the epidemiology and clinical characteristics of P. vivax recurrent infections in India and discuss current knowledge gaps and priority research areas for further investigations, with emphasis on relapses and their radical cure with PQ. Results and discussionA total of 27 studies involving ~27,000 P. vivax infected patients were finally included. Recurrent infections with P. vivax malaria are common, especially in young males. The burden of P. vivax relapse greatly varies across Indian regions, with a proportion range of 1.47 to 6% based on the included studies which all used low (very low) PQ dose. There is a need for more empirical data on the effectiveness and safety of weekly administration of PQ at 0.75 mg/Kg for eight weeks in G6PD–deficiency patients in India, especially in children. Further research priorities should also be focused on the epidemiology of confounding factor, such as CQ-resistance, mixed infections, or Pv genetic diversity are needed. The clinical impact of P. vivax relapses (e.g., severe malaria, mortality) is also of valuable interest in upcoming studies. More studies addressing the above-mentioned missing links should be implemented to inform malariologists, clinicians, populations, and policy makers on real situation of P. vivax relapses and the clinical impact of PQ in India. All taken together, these would have important implications for P. vivax malaria control and elimination in endemic areas.
Myopia prevalence, refractive status and uncorrected myopia among primary and secondary school students in Germany
Authors: Hönekopp Astrid , Tommes Lisa-Marie , Doebler Philipp , Weigelt Sarah 
Published: December 2024

IntroductionThe increasing prevalence of myopia worldwide is problematic because myopia can result in severe secondary pathologies, and is associated with considerable financial burden. With plenty of prevalence data available for some regions, current data for Europe remain sparse. Yet, information on myopia prevalence and associations is essential for monitoring, preventive and interventive purposes. Likewise, uncorrected refractive errors are also critical, as they can, e.g., affect educational outcomes, making information on uncorrected myopia valuable for diagnostics and health education.MethodsWe performed non-cycloplegic autorefraction on two samples in Germany. The younger sample included 489 primary school students (grades 3–4, mean age: 9.30 ± 0.78 years), the older sample 1,032 secondary school students (grades 8–10, mean age 14.99 ± 1.12 years). These samples mark the limits of the age range during which school myopia usually emerges.ResultsMyopia (spherical equivalent ≤ −0.75D) prevalence was 8.4% in the younger sample and 19.5% in the older sample. The prevalence was generally higher in higher grade levels, with the most notable difference between grades 8 and 9. Females were more myopic than males in all grades except grade 3, with the largest gender difference in grade 10. The older sample also exhibited a more myopic spherical equivalent than the younger sample. In the older sample, spherical equivalent was more myopic in females than in males, and in grade 9 and 10 participants more than in grade 8 participants. Rates of uncorrected myopia were extremely high: 51.2% in the younger sample and 43.3% in the older sample.DiscussionThe obtained myopia prevalence rates are generally consistent with other European studies, as is the higher prevalence in female than male adolescents, accelerating with age. The high rates of uncorrected myopia warrant further investigation and should inform public health policies, including the implementation of regular refractive screenings.
Tuberous sclerosis: a survey in the canton of Vaud, Switzerland
Authors: Hagon-Nicod Olivia , Fellmann Florence , Novy Jan , Lebon Sébastien , Wider Christian , Lazor Romain , Bonny Olivier 
Published: December 2024

Aim of the studyTuberous sclerosis complex (TSC) is a genetic and multisystemic disorder that affects between 1/6’000 and 1/10’000 of newborns. Clinical criteria and/or genetic analysis establish the diagnosis. The mechanistic target of rapamycin (mTOR) inhibitors everolimus or sirolimus reduce the severity of several TSC-related clinical traits. We report here on the epidemiology and management of TSC patients in a large Swiss canton: the canton of Vaud.MethodWe extracted patient files containing the diagnostic code TSC in 2015 at the Lausanne University hospital (tertiary reference center for a population of about 755’000 people) and in specialized neurological institutions of the same region.ResultsWe identified 52 patients with a diagnosis of TSC. The majority of the patients with a pathologic result in genetic testing were positive for a pathogenic variant in the TSC2 gene, including five cases of contiguous gene deletion syndrome of TSC2 and PKD1 causing both polycystic kidney disease and TSC. The most frequent clinical manifestations encountered were affecting the skin (87% of patients), the brain (83%), the heart (46%) and the kidneys (46%). Neuropsychiatric disorders were described in 56% of cases. At the time of data collection (2015), there were 2 patients using systemic mTOR inhibitors and 16 patients using topical mTOR inhibitors for dermatological features. Next, we compared this data with those of large published cohorts. While we found fewer cases than expected for the screened population, demographic as well as genetic data were overall similar to the literature. However, we observed that some clinical manifestations (renal, lung and neuropsychiatric disorders) were less frequently described in our cohort.ConclusionThis work indicates that TSC and some of its clinical manifestations is under-reported. It raises concern that patients with mild manifestations are often not referred to reference centers with dedicated multidisciplinary group. The follow-up by expert board is instrumental in offering systematic screening of all putatively affected organs and to assess the eligibility for targeted treatment.
Identification and characterization of a novel chromosome-encoded aminoglycoside O-nucleotidyltransferase gene, ant(9)-Id, in Providencia sp. TYF-12 isolated from the marine fish intestine
Authors: Yu Yan , Zhang Runzhi , Pan Wei , Sheng Xinyi , Chen Susu , Wang Junjun , Lu Junwan , Bao Qiyu , Hu Yunliang , Jiang Pengfei , Huang Dawei 
Published: December 2024

BackgroundThe mechanisms underlying the resistance of the genus Providencia to aminoglycosides are complex, which poses a challenge for the efficient treatment of infectious diseases caused by these pathogens. To help clinicians treat infections more effectively, a more comprehensive understanding of antibiotic resistance mechanisms is urgently needed.MethodsPlates were streaked to isolate bacteria from the intestinal contents of fish. The standard agar dilution method was used to determine the minimum inhibitory concentrations (MICs) of the antimicrobial agents. Molecular cloning was carried out to study the function of the novel antibiotic inactivation gene ant(9)-Id. The kinetic parameters of ANT(9)-Id were measured by a SpectraMax multifunctional microplate reader. Whole-genome sequencing and bioinformatic analysis were conducted to elucidate the sequence structure and evolutionary relationships of similar genes.ResultsThe novel aminoglycoside O-nucleotidyltransferase gene ant(9)-Id was encoded on the chromosome of a species-unclassified isolate designated Providencia sp. TYF-12, which was isolated from the intestine of a marine fish. Among the 11 aminoglycosides tested, ant(9)-Id was resistant to only spectinomycin. The MIC of spectinomycin for the recombinant strain carrying ant(9)-Id (pUCP20-ant(9)-Id/DH5α) increased 64-fold compared with that of the control strain (pUCP20/DH5ɑ). ANT(9)-Id shares the highest amino acid (aa) identity of 46.70% with the known drug resistance enzyme ANT(9)-Ic. Consistent with the MIC results, ANT(9)-Id showed high affinity and catalytic efficiency for spectinomycin, with a Km of 8.94 ± 2.50 μM and a kcat/Km of 26.15 μM−1·s−1. This novel resistance gene and its close homologs are conserved in Providencia strains from various sources, including some of clinical significance. No mobile genetic elements (MGEs) surrounding the ant(9)-Id(−like) genes were identified.ConclusionThis work revealed and characterized a novel spectinomycin resistance gene, ant(9)-Id, along with its biological features. Identifying novel resistance genes in pathogens can assist in rational medication use and the identification of additional antimicrobial resistance mechanisms in microbial populations.
Identification of key factors for malnutrition diagnosis in chronic gastrointestinal diseases using machine learning underscores the importance of GLIM criteria as well as additional parameters
Authors: Rischmüller Karen , Caton Vanessa , Wolfien Markus , Ehlers Luise , van Welzen Matti , Brauer David , Sautter Lea F. , Meyer Fatuma , Valentini Luzia , Wiese Mats L. , Aghdassi Ali A. , Jaster Robert , Wolkenhauer Olaf , Lamprecht Georg , Bej Saptarshi 
Published: December 2024

IntroductionDisease-related malnutrition is common but often underdiagnosed in patients with chronic gastrointestinal diseases, such as liver cirrhosis, short bowel and intestinal insufficiency, and chronic pancreatitis. To improve malnutrition diagnosis in these patients, an evaluation of the current Global Leadership Initiative on Malnutrition (GLIM) diagnostic criteria, and possibly the implementation of additional criteria, is needed.AimThis study aimed to identify previously unknown and potentially specific features of malnutrition in patients with different chronic gastrointestinal diseases and to validate the relevance of the GLIM criteria for clinical practice using machine learning (ML).MethodsBetween 10/2018 and 09/2021, n = 314 patients and controls were prospectively enrolled in a cross-sectional study. A total of n = 230 features (anthropometric data, body composition, handgrip strength, gait speed, laboratory values, dietary habits, physical activity, mental health) were recorded. After data preprocessing (cleaning, feature exploration, imputation of missing data), n = 135 features were included in the ML analyses. Supervised ML models were used to classify malnutrition, and key features were identified using SHapley Additive exPlanations (SHAP).ResultsSupervised ML effectively classified malnourished versus non-malnourished patients and controls. Excluding the existing GLIM criteria and malnutrition risk reduced model performance (sensitivity -19%, specificity -8%, F1-score -10%), highlighting their significance. Besides some GLIM criteria (weight loss, reduced food intake, disease/inflammation), additional anthropometric (hip and upper arm circumference), body composition (phase angle, SMMI), and laboratory markers (albumin, pseudocholinesterase, prealbumin) were key features for malnutrition classification.ConclusionML analysis confirmed the clinical applicability of the current GLIM criteria and identified additional features that may improve malnutrition diagnosis and understanding of the pathophysiology of malnutrition in chronic gastrointestinal diseases.
Ovarian reserve modulates the impact of vitamin D deficiency on assisted reproductive outcomes in patients undergoing controlled ovarian hyperstimulation
Authors: He Lina , Xu Qing , Hao Li , Ran Xu , Qiu Yamin , Lin Jie , Chen Wei 
Published: December 2024

ObjectiveThe association between vitamin D deficiency and ovarian reserve-specific outcomes of assisted reproductive technology (ART) remains uncertain. This study aimed to investigate the role of ovarian reserve in the association between basal serum vitamin D levels and ART outcomes in patients undergoing controlled ovarian hyperstimulation (COH).MethodsA total of 1,333 infertile women undergoing COH cycles were retrospectively analyzed. Patients were divided into a vitamin D deficiency group (serum vitamin D &lt; 20 ng/mL) and a normal vitamin D group. Univariate and multivariate logistic regression analyses were performed to estimate the relationship between vitamin D deficiency and pregnancy outcomes including biochemical pregnancy rate, clinical pregnancy rate, miscarriage rate, and live birth rate in the overall cohort and subgroups with diminished ovarian reserve (DOR) or polycystic ovary syndrome (PCOS).ResultsIn the entire participants’ cohort, no correlation between vitamin D deficiency and pregnancy results was observed (p &gt; 0.05). However, in the DOR subgroup, vitamin D deficiency was associated with a lower biochemical pregnancy rate (adjusted OR 0.27; 95% CI 0.08–0.90; p &lt; 0.01) and clinical pregnancy rate (adjusted OR 0.36; 95% CI 0.14–0.90; p &lt; 0.01). No significant differences were observed in pregnancy outcomes among patients with PCOS (p &gt; 0.05).ConclusionVitamin D deficiency does not affect pregnancy outcomes in the overall patient population, but it may negatively impact women with DOR potentially leading to poorer pregnancy outcomes. Further studies are needed to clarify the mechanisms involved and the potential use of vitamin D screening and supplementation in specific populations.
Inflammation and nutritional status in relation to mortality risk from cardio-cerebrovascular events: evidence from NHANES
Authors: Hou Chengzhi , Huang Xuanchun , Wang Jie , Chen Cong , Liu Chao , Liu Shuyuan , Li Hongping 
Published: December 2024

ObjectiveInflammation and nutritional status are closely associated with the mortality risk of survivors of cardio-cerebrovascular events. This study aims to evaluate the relationship between inflammation and nutritional indices and mortality among, identifying the most predictive indices.MethodsThis study included cohort data of the survivors of major adverse cardiovascular and cerebrovascular events (MACCE) from the National Health and Nutrition Examination Survey (NHANES) in 1999–2010. MACCE is defined as a composite of myocardial infarction, heart failure and stroke, and at least one of the three events occurs. The main outcomes were all-cause mortality and cardiovascular mortality. Kaplan–Meier analysis and receiver operating characteristic curves were used to compare the correlation between seven inflammatory nutritional indices (such as Advanced Lung Cancer Inflammation Index, ALI) and mortality among the survivors. A multivariable-adjusted Cox regression and restricted cubic splines analysis identified the most predictive index, with the optimal number of nodes determined by the Akaike information criterion. Subgroup and sensitivity analyses were conducted to assess model stability.ResultsA total of 2,045 MACCE survivors were included. The higher levels of ALI and serum albumin were significantly associated with lower risks of all-cause and cardiovascular mortality among these individuals. Increases in C-reactive protein to Lymphocyte Ratio, Neutrophil to Serum Albumin Ratio, Neutrophil-to-Lymphocyte Ratio, Systemic Immune-Inflammation Index (SII), and C-reactive protein were similarly correlated with higher mortality risk. ALI outperformed other indices, displaying a distinct L-shaped nonlinear relationship with both all-cause and cardiovascular mortality among MACCE survivors, with an inflection point at 90 indicating the lowest risk. To the left of this inflection, each unit increase in ALI was associated with a 1.3% decrease in all-cause and cardiovascular mortality risk among MACCE patients. To the right, the risk might increase by 0.2%, although the change was not statistically significant. Subgroup analyses and sensitivity analyses showed that the association between ALI and risk of mortality remained stable in most MACCE survivor populations.ConclusionRoutine and dynamic monitoring of ALI is helpful for clinicians to assess the mortality risk among MACCE survivors. Anti-inflammatory therapies and appropriate nutritional support are crucial for reducing mortality in these individuals.
Diagnosis and treatment experience of cecal diverticulitis in six pediatric patients
Authors: Li Xiao , Liu Wei , Zhang Shisong , Liu Hongzhen 
Published: December 2024

ObjectiveThis study aims to summarize the clinical characteristics, diagnostic methods, and treatment experience of cecal diverticulitis in children.MethodThe clinical data of six pediatric patients with cecal diverticulitis, treated at Children's Hospital Affiliated to Shandong University from November 2021 to May 2023, were retrospectively analyzed.ResultAll patients presented with abdominal pain primarily in the lower right abdomen. Two cases had fever with a maximum body temperature not exceeding 38.0°C. Three cases exhibited elevated inflammatory markers such as white blood cell count and C-reactive protein (CRP) upon admission. Three children were misdiagnosed with acute appendicitis based on preoperative color Doppler ultrasound. Two children were treated with third-generation cephalosporins and ornidazole for anti-inflammatory therapy and were cured after 6 and 9 days of hospitalization, respectively. Four children underwent laparoscopic surgery with excision of the cecal diverticulum and cecal repair, all of whom recovered well without postoperative complications. The operation duration ranged from 100 to 170 min, with an average of 140 min. Intraoperative blood loss ranged from 5 to 10 ml, averaging 6.75 ml. The overall length of hospital stay was 8–12 days, with an average stay of 9.5 days. All patients were followed up until December 2023, with no recurrences observed.ConclusionChildren with cecal diverticulitis, especially complex cecal diverticulitis, are easily misdiagnosed as acute appendicitis. Acute simple cecal diverticulitis can be treated with anti-inflammatory therapy. Laparoscopic cecal diverticulectomy combined with cecal repair is a feasible and effective method for treating acute complex cecal diverticulitis.
Cross-sectional and prospective associations between children's eating behavior and visceral fat area and trunk fat mass
Authors: Wang Youxin , Shen Fangjing , Zhang Pingping , Xu Miao , Qiu Danqi , Wang Hui , Li Li 
Published: December 2024

BackgroundUnderstanding children's eating behaviors is crucial for elucidating the etiology of childhood obesity. However, the relationship between these behaviors and abdominal fat accumulation remains poorly understood. This study aims to investigate this association in primary school children.MethodsThis study included 1,475 children aged 8–10 years in Ningbo, China. Body composition was measured both at baseline (September 2022) and after 9 months of follow-up (June 2023). Primary caregivers completed the Children's Eating Behavior Questionnaire (CEBQ). A mixed-effects linear model was applied to analyze the relationships between children's eating behaviors and body composition.ResultsGreater visceral fat area (VFA) was associated with greater slowness in eating [β = 0.25 (0.02, 0.49)], greater food responsiveness [β = 0.29 (0.14, 0.43)], increased emotional undereating [β = 0.35 (0.17, 0.54)], and more emotional overeating [β = 0.21 (0.02, 0.39)]. Children with greater trunk fat mass (TrFM) tended to have greater food responsiveness [β = 0.02 (0.01, 0.03)] and greater enjoyment of food [β = 0.01 (0.00, 0.03)], increased emotional undereating [β = 0.02 (0.01, 0.03)] and emotional overeating [β = 0.02 (0.00, 0.03)]. Prospectively, positive correlations were observed between VFA and food responsiveness [β = 0.18 (0.02, 0.35)], as were emotional undereating [β = 0.31 (0.10, 0.52)] and emotional overeating [β = 0.24 (0.03, 0.46)]. Similarly, TrFM was positively correlated with food responsiveness [β = 0.02 (0.00, 0.03)], enjoyment of food [β = 0.02 (0.00, 0.04)], emotional undereating [β = 0.02 (0.01, 0.04)] and emotional overeating [β = 0.02 (0.01, 0.04)].ConclusionsOur findings suggest that eating behaviors are significantly associated with abdominal fat accumulation in primary school children. Addressing specific eating behaviors may be crucial in mitigating abdominal fat and its related health risks.