bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pilot‐scale open‐channel raceways and flat‐panel photobioreactors maintain well‐mixed conditions under a wide range of mixing energy inputs
Authors: Quiroz‐Arita Carlos , Blaylock Myra L. , Gharagozloo Patricia E. , Bark David , Prasad Dasi Lakshmi , Bradley Thomas H. 
Published: January 2020

AbstractTurbulent mixing in pilot‐scale cultivation systems influences the productivity of photoautotrophic cultures. We studied turbulent mixing by applying particle image velocimetry and acoustic doppler velocimetry to pilot‐scale, flat‐panel photobioreactor, and open‐channel raceway. Mixing energy inputs were varied from 0.1 to 2.1 W·m−3. The experimental results were used to quantify turbulence and to validate computational fluid dynamics models, from which Lagrangian representations of the fluid motion in these reactors were derived. The results of this investigation demonstrated that differences in mixing energy input do not significantly impact the structure of turbulence and the light/dark cycling frequencies experienced by photoautotrophic cells within the reactors. The experimental and computational results of our research demonstrated that well‐mixed conditions exist in pilot‐scale, flat‐panel photobioreactors and open‐channel raceways, even for relatively low mixing energy inputs.
The role of oxytocin on self‐serving lying
Authors: Sindermann Cornelia , Luo Ruixue , Becker Benjamin , Kendrick Keith M. , Montag Christian 
Published: January 2020

AbstractIntroductionThe effects of intranasal administration of the neuropeptide oxytocin on social cognition and behavior are highly specific. Potentially situational and personal variables influence these effects. The aim of the present study was to investigate effects of oxytocin administration on self‐serving lying, including situational effects.MethodsA total of 161 adult males participated in a randomized double‐blind placebo‐controlled between‐subject intranasal oxytocin administration (24 international units) study. Self‐serving lying was assessed using three subsequent rounds of the die‐in‐a‐cup paradigm, in which different degrees of lying can be implemented by the participants that can be determined on group level.ResultsOxytocin administration seemed to promote self‐serving lying, particularly in the third (last) round and only to a certain degree (not to the maximum possible).ConclusionsOur findings demonstrate that oxytocin administration can promote self‐serving lying when given repeated opportunities to lie. Moreover, exploratory results presented in the Supplementary Material indicate that the sensitivity to the effects of intranasal oxytocin in this domain might be moderated by individual differences in the oxytocin receptor gene.
Effective temperature shift strategy development and scale confirmation for simultaneous optimization of protein productivity and quality in Chinese hamster ovary cells
Authors: McHugh Kyle P. , Xu Jianlin , Aron Kathryn L. , Borys Michael C. , Li Zheng Jian 
Published: January 2020

AbstractTemperature shifts to lower culture temperatures are frequently employed in the manufacturing of protein therapeutics in mammalian cells to improve productivity, viability, or quality attributes. The direction and extent to which a temperature shift affects productivity and quality may vary depending on the expression host and characteristics of the expressed protein. We demonstrated here that two Chinese hamster ovary (CHO) clones expressing different human monoclonal antibodies responded differently to a temperature shift despite sharing a common parental CHO cell line. Within a single CHO line, we observed a nonlinear response to temperature shift. A moderate shift to 35°C significantly decreased final titer relative to the unshifted control while a larger shift to 32°C significantly increased final titer by 25%. Therefore, we proposed a systematic empirical approach to assess the utility of a temperature shift for faster implementation during process development. By testing multiple shift parameters, we identified optimum shift conditions in shake flasks and successfully translated findings to benchtop bioreactors and 1,000‐L bioreactor scale. Significant differences in final antibody titer and charge variants were observed with temperature shift increments as small as Δ1.5°C. Acidic charge variants decreased monotonically with decreasing shift temperature in both cell lines; however, final antibody titer required simultaneous optimization of shift day and temperature. Overall, we were able to show that a systematic approach to identify temperature shift parameters at small scales is useful to optimize protein production and quality for efficient and confident translation to large‐scale production.
Generation of keratinocyte stem‐like cells from human fibroblasts via a direct reprogramming approach
Authors: Zhao Andong , Yang Yi , Pan Xiaohua , Pan Yu , Cai Sa 
Published: January 2020

AbstractSkin repair and reconstruction are important after severe wound and trauma. Keratinocyte stem cells (KSCs) in the basal layer of the epidermis can regrow the stratified epidermis but are almost depleted after skin injury. Thus, generating enough KSCs is indispensable for skin regeneration. Pluripotent stem cells such as ESC and iPSC can differentiate into KSCs, but their applications are challenged by ethical issues and risks of tumor formation. Lineage reprogramming from one cell type into another one makes it feasible to generate the desired cell type. Here, we develop a method to convert human fibroblasts into induced keratinocyte stem‐like cells (iKSC) by coupling transient expression of reprogramming factors with a chemically defined culture medium, without the formation of iPSC. iKSC resemble normal KSC in the morphological and phenotypic features and can differentiate in vitro and regenerate stratified epidermis after transplantation in vivo. Therefore, iKSC may provide abundant cellular sources for skin repair and regeneration.
Why did I buy this? Consumers' post‐impulse‐consumption experience and its impact on the propensity for future impulse buying behaviour
Authors: Spiteri Cornish Lara 
Published: January 2020

AbstractThis paper advances research into impulse buying by examining how this behaviour is reinforced/curtailed, highlighting the primary role of the post‐purchase experience in encouraging future impulse buying behaviour. Extant research emphasizes the role of the purchase experience, that is, psychological benefits attained from the impulse purchase experience itself, as a reinforcer of impulse buying. Conversely, this paper uses experiments to demonstrate that it is the post‐purchase experience, not the purchase experience, that reinforces or curtails future impulse buying. Indeed, irrespective of the valence of the purchase experience, a negative post‐purchase experience (whereby a product or service is found to have limited use/does not deliver expected benefits) results in post‐purchase regret, which in turn curtails future impulse buying. In contrast, a positive post‐purchase experience reinforces impulse buying. This research also demonstrates that consumers utilize three types of coping mechanisms to mitigate post‐purchase regret, that is, planful problem solving, positive reinterpretation, or mental disengagement. However, although the use of planful problem solving curtails future impulse buying, use of the other two mechanisms results in behaviour reinforcement. These findings have several important implications for both marketers and consumers, which the authors discuss in detail.
The role of storytelling in advertising: Consumer emotion, narrative engagement level, and word‐of‐mouth intention
Authors: Kang Jin‐Ae , Hong Sookyeong , Hubbard Glenn T. 
Published: January 2020

AbstractThis experimental study with a national online sample (n = 300) tested the effects of storytelling in radio advertisements on participants' positive emotional responses and intentions to share information about the product, depending on audiences' narrative engagement level. Treatments included a commercial for a fictitious brand of luggage using a story told by the founder of the company, another version of the same commercial manipulated so the speaker was a customer of the company, and a control stimulus consisting only of information about the product. Results showed that narrative transportation and narrative preference are positively associated with favorable responses toward ad. Stories elicited more favorable emotional responses and had some effect on participants' intention to share information about the product by word‐of‐mouth. This was especially true among participants hearing the founder's story. Results support previous assumptions about the power of storytelling in advertising, including distinctions regarding the identity of the speaker (founder vs. customer).
Outcomes of intravascular brachytherapy for recurrent drug‐eluting in‐stent restenosis
Authors: Megaly Michael , Glogoza Matthew , Xenogiannis Iosif , Vemmou Evangelia , Nikolakopoulos Ilias , Willson Laura , Monyak David J. , Sullivan Patsa , Stanberry Larissa , Sorajja Paul , Chavez Ivan , Mooney Michael , Traverse Jay , Wang Yale , Garcia Santiago , Poulose Anil , Burke Martin Nicholas , Brilakis Emmanouil S. 
Published: January 2020

AbstractObjectivesTo examine the outcomes of vascular brachytherapy (VBT) for recurrent drug‐eluting stents (DES) in‐stent restenosis (ISR).BackgroundRecurrent DES‐ISR can be challenging to treat. VBT has been used with encouraging results.MethodsWe report the long‐term outcomes of patients with recurrent DES‐ISR treated with VBT between January 2014 and September 2018 at a tertiary care institution. The main outcome was target lesion failure (TLF), defined as the composite of clinically driven target lesion revascularization (TLR), target lesion myocardial infarction (MI), and target lesion‐related cardiac death. Cox proportional hazards analysis was performed to identify variables associated with recurrent TLF.ResultsDuring the study period, 116 patients (143 lesions) underwent VBT. Median follow‐up was 24.7 (14.5–35.4) months. The incidence of TLR, target‐lesion MI, and TLF was 18.9%, 5.6%,and 20.1% at 1 year, and 29.4%, 10.5%, and 32.9% at 2 years.Initial presentation with acute coronary syndrome (ACS) was independently associated with TLF (hazard ratio = 1.975, 95% CI [1.120, 3.485], p = .019). Lesions treated with intravascular ultrasound (IVUS) guidance had a lower incidence of TLR (14.3% vs. 39.6%, log‐rank p = .038), and a trend toward lower incidence of TLF (19% vs. 42.6%, log‐rank p = .086).ConclusionsVBT can improve the treatment of recurrent DES‐ISR, but TLF occurs in approximately one in three patients at 2 years. Initial presentation with ACS was associated with higher TLF and the use of IVUS with a trend for lower incidence of TLF.
The importance of the Heart Team evaluation before transcatheter aortic valve replacement: Results from the BRAVO‐3 trial
Authors: Camaj Anton , Claessen Bimmer E. , Mehran Roxana , Yudi Matias B. , Power David , Baber Usman , Hengstenberg Christian , Lefevre Thierry , Van Belle Eric , Giustino Gennaro , Guedeney Paul , Sorrentino Sabato , Kupatt Christian , Webb John G. , Hildick‐Smith David , Hink Hans U. , Deliargyris Efthymios N. , Anthopoulos Prodromos , Sharma Samin K. , Kini Annapoorna , Sartori Samantha , Chandrasekhar Jaya , Dangas George D. 
Published: January 2020

AbstractBackground/ObjectivesClinicians use validated scores to risk‐stratify patients undergoing transcatheter aortic valve replacement (TAVR). However, evaluation by the Heart Team often deems patients to be at higher risk than their formal scores suggest. We sought to assess clinical outcomes of TAVR patients defined as high‐risk by the Heart Team's assessment versus the patient's logistic EuroSCORE (LES).MethodsThe BRAVO‐3 trial randomized patients at high risk (LES ≥ 18, or deemed inoperable by the Heart Team) to TAVR with periprocedural anticoagulation with unfractionated heparin versus bivalirudin. Endpoints included net adverse cardiac events (NACE: the composite of all‐cause mortality, MI, stroke, or bleeding), major adverse cardiovascular events (MACE: death, MI, or stroke), the individual components of MACE, major vascular complications, BARC ≥ 3b bleeding and VARC life‐threatening bleeding at 30 days. We compared patients deemed high‐risk based on LES ≥ 18 versus high‐risk by the Heart Team despite lower LES.ResultsA total of 467/800 (58.4%) patients were deemed high‐risk by the Heart Team despite LES < 18. After multivariable analysis, there were no differences in the odds of endpoints between groups (NACE, ORLES≥18: 1.32, 95% CI 0.86‐2.02, p = .21; MACE, ORLES≥18: 1.27, 95% CI 0.72‐2.25, p = .41; major vascular complications, ORLES≥18: 0.97, 95% CI 0.65‐1.44, p = .88; BARC ≥3b, ORLES≥18: 1.38, 95% CI 0.82‐2.33, p = .23; and VARC life‐threatening bleeding, ORLES≥18: 0.99, 95% CI 0.69‐1.41, p = .95).ConclusionPatients undergoing TAVR and labeled high‐risk by LES ≥ 18 or Heart Team assessment despite LES < 18 have comparable short‐term outcomes. Assignment of high‐risk status to over 50% of patients is attributable to Heart Team's clinical assessment.
Catalytic Dry Reforming of Methane: Insights from Model Systems
Authors: Wittich Knut , Krämer Michael , Bottke Nils , Schunk Stephan Andreas 
Published: January 2020

AbstractCatalytic dry reforming under industrially relevant conditions of high pressures and high temperatures poses severe challenges towards catalyst materials and process engineering. The demanding conditions under which the reaction is performed lead to a coupling of reactions occurring in the gas phase and reactions which are catalyzed by the material employed as catalyst. A profound analysis of the mechanisms occurring in gas phase and resulting products from gas phase reactions is key to understanding part of the challenges that any catalyst material, irrespective of its nature, will have to cope with. The deposition of coke on an active catalyst is as well one of the most limiting factors for catalyst lifetime and catalyst activity in dry reforming. Therefore, an understanding of the thermodynamics behind coke formation and an intricate description of the mechanisms driving the evolution of coke is a vital piece of the picture. Acid‐base properties of the catalyst material and the role and nature of the active metal do also need to be considered. A large part of the review deals with mechanisms which are relevant for coke gasification and insights into materials properties, which are relevant to allow for reaction pathways along these lines. The review article focusses on research results which have been achieved using model systems – typically the analysis of model systems is a more rewarding exercise compared to fully formulated industrial catalyst systems, as here more elucidating structure‐property relationships can be drawn. Additionally the article discusses dry methane reforming in the context of alternative syngas generation technologies and attempts to create an application perspective for the reader in the context of a sustainable approach towards carbon capture and storage.
An Inexpensive, Efficient Alternative to NADPH to Support Catalysis by Thermostable Cytochrome P450 Enzymes
Authors: Strohmaier Silja J. , Baek Jong Min , De Voss James J. , Jurva Ulrik , Andersson Shalini , Gillam Elizabeth M. J. 
Published: January 2020

AbstractCytochrome P450 enzymes (P450s) are highly desirable catalysts for the regio‐ and stereo‐selective, late‐stage functionalization of pharmaceuticals and other fine chemicals. Recently, the resurrected ancestors of drug‐metabolizing P450s were shown to be highly thermostable and expressed in high yield, while retaining similar substrate specificity to the extant forms. However, they still rely on NADPH and cytochrome P450 reductase (CPR) to enable catalysis of oxidative transformations. To identify an alternative support system, we screened 10 oxygen surrogates (OSs) for the ability to support P450 ancestors from three different families. Of the 23 ancestors examined, 17 were supported by at least one OS as well as, or better than, by CPR. Using two candidate P450s we showed that OS‐dependent P450 catalysis can be optimized in a few steps, boosting product yield from ∼2.2 % with CPR to 88–100 % with an OS. The principles applied here will facilitate faster evaluation and optimization of OS‐supported P450 catalysis versus redox partner‐dependent P450 catalysis.
Poly(vinyl alcohol)/Carboxyl Graphene Membranes for Ethanol Dehydration by Pervaporation
Authors: Han Guanglu , Chen Zhe , Cai Lifang , Zhang Yonghui , Tian Junfeng , Ma Huanhuan , Fang Shaoming 
Published: January 2020

AbstractCarboxyl graphene (CG) with two functions of hybridization and crosslinking was incorporated into poly(vinyl alcohol) (PVA) matrix to form PVA/CG mixed‐matrix membranes (MMMs). The membranes demonstrated excellent mechanical properties and thermal stability. The improved hydrophilicity and formed crosslinking structure led to moderate swelling. The membrane crystallinity decreased and the free volume was promoted with increasing CG loading amount. The pervaporation (PV) separation performance for ethanol dehydration indicated that both permeation flux and separation factor were enhanced simultaneously at the optimum CG loading. Subsequently, the permeation flux continued to increase while the separation factor declined at higher CG loadings.
Electrochemiluminescence of Glutathione‐Stabilized Au Nanoclusters Fractionated by Gel Electrophoresis in Water
Authors: Kang Yunjeong , Kim Joohoon 
Published: January 2020

AbstractWe report the electrochemiluminescence (ECL) of water‐soluble individual Au nanoclusters (Au NCs) fractionated by polyacrylamide gel electrophoresis of Au NCs synthesized using glutathione. The individual Au NCs demonstrated unique and significant differences in ECL. Interestingly, they exhibited near‐infrared (near‐IR) ECL that became dominant when the ratio of Au(I) to Au(0) decreased in the individual Au NCs. This indicated that the oxidation states of Au NCs primarily affected the ECL wavelengths of the clusters because the Au(0)‐glutathionate motif in the Au NCs was responsible for the near‐IR ECL emission.
Azobenzene Isomerization‐Induced Photomodulation of Electronic Properties of N‐Heterocyclic Carbenes
Authors: Islam Sk Aminul , Kundu Kshama , Kundu Pintu K. 
Published: January 2020

AbstractAzobenzene‐based protonated N‐heterocyclic carbenes (NHCs), N,N’‐bis(azobenzene)imidazolium chlorides (IAz‐X⋅HCl; X=OMe, Br, H) were successfully synthesized and switching abilities of the attached azobenzene units along with the concomitant photoinduced generation of geometric isomers were studied. Upon irradiation with 365 nm UV light, a p‐methoxy‐azobenzene derivative get transformed from all‐trans isomer to nearly all‐cis isomer at the photostationary state. The extent of photomodulation of electronic properties in the NHC ring of the p‐methoxy‐azobenzene derivative is determined through the Tolman Electronic Parameter (TEP). Iridium complex, [(IAz‐OMe)IrCl(CO)2] was synthesized and infrared spectra were recorded in dichloromethane solution as film in NaCl crystals and by drop‐casting in an ATR crystal. Comparison in averaged carbonyl stretching frequency between all‐trans isomer (

) and all‐cis isomer (

) indicates a significant decrement of Δtt–cc
av=2.7 cm−1 (film) and 3.8 cm−1 (ATR). Therefore, moderate to excellent enhancement of electron density (Δtt–cc TEP=2.3 cm−1 [film] and 3.2 cm−1 [ATR]) at the C‐2 position of the NHC is achieved through trans→cis isomerization of the remotely placed azobenzene units. This fine phototuning of electron‐donating ability at the catalytic center would pave the way to control NHC/NHC‐metal catalyzed organic transformations through external stimuli.
Short‐term mortality risks among patients with oropharynx cancer by human papillomavirus status
Authors: Fullerton Zoe H. , Butler Santino S. , Mahal Brandon A. , Muralidhar Vinayak , Schoenfeld Jonathan D. , Tishler Roy B. , Margalit Danielle N. 
Published: January 2020

BackgroundThere is substantial variation in head and neck cancer (HNC) mortality and competing mortality among patients with HNC. In this study, the authors characterize the causes and risks of short‐term mortality among patients with oropharynx cancer (OPC) and how these risks differ by human papillomavirus (HPV) status.MethodsA custom Surveillance, Epidemiology, and End Results (SEER) data set with HPV status was used to identify 4930 patients with OPC who were diagnosed with nonmetastatic (M0) disease from 2013 to 2014, including 3560 (72.2%) HPV‐positive patients and 1370 HPV‐negative patients. Causes of death and cumulative incidence estimates for HNC‐specific mortality, competing mortality, second‐cancer mortality, and noncancer mortality were analyzed by HPV status. Risk factors for mortality events were determined using multivariable competing risk regression models.ResultsCompared with HPV‐negative patients, HPV‐positive patients had a lower risk of 2‐year cumulative incidence of all‐cause mortality (10.4% vs 33.3%; P < .0001) and a lower risk of both HNC‐specific mortality (4.8% vs 16.2%; P < .0001) and competing‐cause mortality (5.6% vs 16.8%; P < .0001). Second‐cancer mortality was the most common cause of non‐HNC mortality among HPV‐negative patients. Both second‐cancer mortality and noncancer mortality were significantly higher among patients who had HPV‐negative OPC (10.8% and 6.1%, respectively) compared with those who had HPV‐positive OPC (2.4% and 3.2%, respectively; both P < .0001). The median follow‐up was 11 months (range 1‐23 months) in this cohort with known HPV‐status.ConclusionsPatients with HPV‐positive and HPV‐negative OPC have significantly different rates of both HNC mortality and competing mortality. HPV‐negative patients are at substantial risk of competing mortality, even within 2 years of cancer diagnosis. These differences can inform power calculations for clinical trials and patient management in the acute and survivorship settings.
Testing the differences between two color measurement probability distributions using Hotelling's T2 test and the permutation test
Authors: Gamm Brian M. , Gerard Patrick 
Published: January 2020

AbstractIt is common practice in statistics to test the equality of two population means using, for example, the Student's t test, in the univariate case, or the Hotelling's
T2 Test in the multivariate case. However, tests on the equality of population means are not well developed for testing the difference between two populations of color measurements. Methods for analyzing populations of spectral reflectance and L*a*b* measurements have been described for applications such as analyzing inter‐instrument agreement and repeatability. Methods have also been proposed for the analysis of color differences, but there are little written about techniques for testing whether two samples have the same probability distribution. This article focuses on testing the difference between color measurement probability distributions based on color difference. In addition, a metric is proposed called the threshold for color difference discrimination (TCDD, in units of ΔE), the color difference at which two populations can be considered to have different population distributions. A lower TCDD means smaller color differences between two samples can be resolved. Two parametric tests based on Hotelling's
T2 test and a nonparametric permutation test were used to determine the TCDD for populations of color measurements with different variances and sample sizes. The TCDD was found to be smaller by tests using the Hotelling's
T2 statistic, compared with a permutation test performed directly on color difference. It was also found, as expected, that larger sample sizes led to smaller TCDDs, as did smaller population variances.