bluuebunny/crossref_metadata_2025_split huggingface dataset:
Functional and transcriptional profiling of non-coding RNAs in yeast reveal context-dependent phenotypes and widespreadin transeffects on the protein regulatory network
Authors: Balarezo-Cisneros Laura Natalia , Parker Steven , Fraczek Marcin G , Timouma Soukaina , Wang Ping , O’Keefe Raymond T , Millar Catherine B , Delneri Daniela 
Published: April 2020

AbstractNon-coding RNAs (ncRNAs), including the more recently identified Stable Unannotated Transcripts (SUTs) and Cryptic Unstable Transcripts (CUTs), are increasingly being shown to play pivotal roles in the transcriptional and post-transcriptional regulation of genes in eukaryotes. Here, we carried out a large-scale screening of ncRNAs inSaccharomyces cerevisiae, and provide evidence for SUT and CUT function. Phenotypic data on 372 ncRNA deletion strains in 23 different growth conditions were collected, identifying ncRNAs responsible for significant cellular fitness changes. Transcriptome profiles were assembled for 18 haploid ncRNA deletion mutants and 2 essential ncRNA heterozygous deletants. Guided by the resulting RNA-seq data we analysed the genome-wide dysregulation of protein coding genes and non-coding transcripts. Novel functional ncRNAs, SUT125, SUT126, SUT035 and SUT532 that actin transby modulating transcription factors were identified. Furthermore, we described the impact of SUTs and CUTs in modulating coding gene expression in response of different environmental conditions, regulating important biological process such as respiration (SUT125, SUT126, SUT035, SUT432), steroid biosynthesis (CUT494, SUT530, SUT468) or rRNA processing (SUT075 and snR30). Overall, this data captures and integrates the regulatory and phenotypic network of ncRNAs and protein coding genes, providing genome-wide evidence of the impact of ncRNAs on cellular homeostasis.Author SummaryThe yeast genome contains 25% of non-coding RNA molecules (ncRNAs), which do not translate into proteins but are involved in regulation of gene expression. ncRNAs can affect nearby genes by physically interfering with their transcription (cismode of action), or they interact with DNA, proteins or others RNAs to regulate the expression of distant genes (transmode of action). Examples ofcis-acting ncRNAs have been broadly described, however genome-wide studies to identify functionaltrans-acting ncRNAs involved in global gene regulation are still lacking. Here, we used the ncRNA yeast deletion collection to score their impact on cellular function in different environmental conditions. A group of 20 ncRNAs mutants with broad fitness diversity were selected to investigate their effect on the protein and ncRNA expression network. We showed a high correlation between altered phenotypes and global transcriptional changes, in an environmental dependent manner. We confirmed the widespreadtransacting expressional regulation of ncRNAs in the genome and their role in affecting transcription factors. These findings support the notion of the involvement on ncRNAs in fine tuning the cellular expression via regulations of TFs, as an advantageous RNA-mediated mechanism that can be fast and cost-effective for the cells.
Temporal analysis of localization and trafficking of glycolipids
Authors: Arai Kenta , Kabayama Kazuya , Kanie Yoshimi , Kanie Osamu , Fukase Koichi 
Published: April 2020

AbstractGlycolipid metabolism occurs in the Golgi apparatus, but the detailed mechanisms have not yet been elucidated. We used fluorescently labeled glycolipids to analyze glycolipid composition and localization changes and shed light on glycolipid metabolism. In a previous study, the fatty chain of lactosyl ceramide was fluorescently labeled with BODIPY (LacCer-BODIPY) before being introduced into cultured cells to analyze the cell membrane glycolipid recycling process. However, imaging analysis of glycolipid recycling is complicated because of limited spatial resolution. Therefore, we examined the microscopic conditions that allow the temporal analysis of LacCer-BODIPY trafficking and localization. We observed that the glycolipid fluorescent probe migrated from the cell membrane to intracellular organelles before returning to the cell membrane. We used confocal microscopy to observe co-localization of the glycolipid probe with endosomes and Golgi markers, demonstrating that it recycles mainly through the trans-Golgi network (TGN). Here, a glycolipid recycling pathway was observed that did not require the lipids to pass through the lysosome.
AthCNV: A Map of DNA Copy Number Variations in the Arabidopsis Genome
Authors: Zmienko Agnieszka , Marszalek-Zenczak Malgorzata , Wojciechowski Pawel , Samelak-Czajka Anna , Luczak Magdalena , Kozlowski Piotr , Karlowski Wojciech M. , Figlerowicz Marek 
Published: April 2020

Abstract
Copy number variations (CNVs) greatly contribute to intraspecies genetic polymorphism and phenotypic diversity. Recent analyses of sequencing data for &gt;1000 Arabidopsis (Arabidopsis thaliana) accessions focused on small variations and did not include CNVs. Here, we performed genome-wide analysis and identified large indels (50 to 499 bp) and CNVs (500 bp and larger) in these accessions. The CNVs fully overlap with 18.3% of protein-coding genes, with enrichment for evolutionarily young genes and genes involved in stress and defense. By combining analysis of both genes and transposable elements (TEs) affected by CNVs, we revealed that the variation statuses of genes and TEs are tightly linked and jointly contribute to the unequal distribution of these elements in the genome. We also determined the gene copy numbers in a set of 1060 accessions and experimentally validated the accuracy of our predictions by multiplex ligation-dependent probe amplification assays. We then successfully used the CNVs as markers to analyze population structure and migration patterns. Finally, we examined the impact of gene dosage variation triggered by a CNV spanning the SEC10 gene on SEC10 expression at both the transcript and protein levels. The catalog of CNVs, CNV-overlapping genes, and their genotypes in a top model dicot will stimulate the exploration of the genetic basis of phenotypic variation.
Determinant factors of climate change adaptation by pastoral/agro-pastoral communities and smallholder farmers in sub-Saharan Africa
Authors: Menghistu Habtamu Taddele , Abraha Amanuel Zenebe , Tesfay Girmay , Mawcha Gebrehiwot Tadesse 
Published: April 2020

PurposeThe purpose of this systematic review was to assess the determinant factors of climate change adaptation (CCA) in sub-Saharan Africa (SSA).Design/methodology/approachStudies that focused on determinant factors of CCA by crop–livestock farmers and pastoralists in SSA and written in English were reviewed from five major databases using the applications of Endnote and NVivo. The review process followed a sequence of steps to reach into the final selection.FindingsA total of 3,028 papers were recovered from the databases and screened for duplicates (777) and publications before 2000 (218). The titles and abstracts of 2,033 papers were reviewed, and 1,903 of them were excluded owing to preliminary exclusion criteria. Finally, 130 papers were selected for full-text review and more detailed assessment, where 36 papers qualified for the final review. The most important determinant factors of CCA by pastoralists were household income, access to information, access to extension services, government support and access to market. In the case of agro-pastoralists, access to information, household income, age and land/livestock ownership were found as the major determinant factors. Household income, land ownership, access to information, farm size, household size and access to extension services were the determinant factors found for CCA by smallholder farmers.Research limitations/implicationsThis systematic review identified the major determinant factors according to production systems and highlights the importance of considering specific factors in designing CCA strategies.Originality/valueAfter clearly stating the research question, a literature search was conducted from the major databases for climate-related research, and a comprehensive search was performed by two independent researchers.
Institutional change of cloud ERP implementation in the public sector
Authors: Alsharari Nizar Mohammad 
Published: April 2020

Purpose
This study aims to explain the transformation process from using regular enterprise resource planning (ERP) system into implementing Cloud ERP system in the UAE public sector.

Design/methodology/approach
The study uses a qualitative case study and analyzes the primary evidences from in-depth interviews with the case organization’s personnel. It conducts a thematic analysis of the interviews’ findings. Furthermore, the study uses secondary and tertiary resources from published sources comprising the case organization’s website and previous studies.

Findings
The findings demonstrated that the transformation process to Cloud ERP could result in different practical benefits in an organization’s controlling system, cost reduction and profitability. Conversely, results revealed that the effectiveness of implementing cloud ERP is dependable on the provider’s professionalism; hence resulting in issues related to minimized organizational independence. In addition, the paper’s findings provide evidenced clarifications about the controversial misconceptions of Cloud ERP’s privacy issues.

Research limitations/implications
The paper is exposed to the regular case studies limitations, as the study is a qualitative research studying one case company. Thus, the study findings should be strengthened by future quantitative researches on more companies with implying more statistical analysis.

Practical implications
The paper has important implications for practitioners and decision-makers, as it presents significant and reality-based information about Cloud ERP implementation’s benefits and drawbacks. It thus enhances decision-makers’ ability to make an appropriate and suitable decision about adopting the Cloud ERP in the public sector.

Originality/value
As Cloud ERP is still emerging, this study is one of the very few case studies that discuss and present some experienced benefits and issues related to Cloud ERP implementation in UAE Government. This study also uses transformation theory to analyze the data.
Antecedents to buyer-supplier coordination in the pharmaceutical supply chain
Authors: Jambulingam Thanigavelan , Kathuria Ravi 
Published: April 2020

PurposeThe purpose of this study is to understand the antecedents that influence supply chain coordination in the pharmaceutical supply chain using the transaction cost analysis framework.Design/methodology/approachData from 156 retail pharmacies on their relationship with the pharmaceutical wholesalers are used to test the hypotheses.FindingsThe findings of this paper show the importance of antecedents that are based on the transactional cost theory, such as asset specificity and environmental uncertainty. These antecedents impact the supply chain process coordination at different levels – transactional, operational and strategic.Research limitations/implicationsFuture research may investigate additional antecedents using other theoretical lenses.Practical implicationsPharmaceutical wholesalers are dependent on pharmaceutical manufacturers for the supply of products and face intense competition that results in lower profit margins. Given that the pharmaceutical industry is strictly regulated, the wholesaler facilitates regulatory compliance of the manufacturers in the distribution process by coordinating with them. But the wholesalers do also face a constant threat from the manufacturers, who could potentially bypass the wholesalers (disintermediation) and go directly to the pharmacies. To counterbalance the dependence, the wholesalers strive to achieve loyalty with the retail pharmacies. Through supply chain coordination, the wholesalers achieve efficiency in procurement for the pharmacies, thus reducing cost and improving their competitive advantage.Social implicationsSupply chain coordination in the pharmaceutical supply chain improves the safety and security of the pharmaceutical distribution system.Originality/valueThis paper contributes to the supply chain coordination stream of literature. To the best of the authors’ knowledge, this is the first study to develop the three levels of process coordination in the pharmaceutical supply chain context. This paper shows how process coordination can be achieved between the dyad without vertical integration.
Social capital affects job performance through social media
Authors: Yen Yung-Shen , Chen Mei-Chun , Su Chun-Hsiung 
Published: April 2020

PurposeThis study aims to explore the impact of social capital on job performance when workers interact with coworkers through social media in organizations.Design/methodology/approachStructural equation modeling was conducted, and a sample of 230 workers in Taiwan was investigated.FindingsThis study found that bonding social capital has a greater impact on job performance than bridging social capital for interactions among coworkers through social media in organizations. Moreover, bridging social capital affects job performance more strongly for male workers than for female workers, but bonding social capital affects job performance more strongly for female workers than for male workers.Research limitations/implicationsThis study extended social capital theory by adding the mediating effects of job satisfaction and relational satisfaction and the moderating effect of gender into the model.Practical implicationsThis study suggests that company managers need to train workers how to use social media to appropriate their affordances and consider the work team relationship to position adequate strategies for male and female workers.Originality/valueThis study advances the previous knowledge of social capital theory for workers interacting with coworkers through social media in organizations.
OKC-enabled online knowledge integration: role of group heterogeneity and group interaction process
Authors: Qiu Jiangnan , Xu Liwei , Zuo Min , Wang Jingxian , Helen Weadon 
Published: April 2020

PurposeOnline knowledge integration has been an important concern of the online knowledge community as it can lead to various positive outcomes of online knowledge coproduction. This paper identifies online knowledge integration factors by considering group heterogeneity and group interaction process.Design/methodology/approachBased on the categorization-elaboration model (CEM) and interactive team cognition (ITC) theory, a research model that reflects the antecedent's factors and mediating factors of online knowledge integration was developed and empirically examined based on data collected from 2,339,836 data extracted from Wikipedia.FindingsGroup interaction process plays an essential mediator role in online knowledge integration. Group knowledge heterogeneity negatively influences online knowledge integration and group experience heterogeneity positively, and they both positively promote online knowledge integration through group interaction process with different paths.Research limitationsOur research concerns the OKC context in one setting (Wikipedia). We expect that the results will generalize to other OKC platforms.Practical implicationsThe findings of the study could assist the online knowledge community's organizers to understand the motivational mechanisms of online knowledge integration. Group interaction process could be regarded as the key role to promote group wisdom and maintain group independence.Social implicationsWe advance the understanding of the online knowledge integration and gain a richer understanding of the importance of group interaction independence for online knowledge integration based on the agreement of group wisdom. It suggested keeping group interaction independence is an important aspect for highly online knowledge integration among heterogeneity groups.Originality/valueThis study extends CEM and ITC theory to the domain of knowledge integration context and finds the mechanism between group heterogeneity and online knowledge integration by introducing the group interaction process.
The impact of telematics on the insurability of risks
Authors: Eling Martin , Kraft Mirko 
Published: April 2020

Purpose
The purpose of this paper is to analyze the use of telematics in insurance and its consequences for the insurability of risks. Empirical results on monitoring policyholders or insured objects and its consequences for asymmetric information, as well as claims frequency and severity are discussed. Furthermore, potential future research questions that arise from the use of telematics in risk management and insurance are outlined.

Design/methodology/approach
The paper systematically reviews existing studies and then investigates the consequences of telematics using Berliner’s insurability criteria. The results are based on 52 academic studies and industry papers published from 2000 to 2019.

Findings
The findings emphasize the effects of new information on information asymmetry and risk pooling, the implications of new technologies on loss frequency and severity, legal restrictions and ethical consequences of the use of telematics in the insurance field. Problems with the insurability impede the market development of innovations such as telematics tariffs.

Originality/value
Despite its increasing relevance for businesses at present, research on telematics in insurance is limited. Some papers can be found in the IT domain, but relatively little research has been done in the business and economics literature. The authors illustrate where the research stands currently and outline directions for future research.
AI voice bots: a services marketing research agenda
Authors: Klaus Phil , Zaichkowsky Judy 
Published: April 2020

Purpose
This paper aims to document how AI has changed the way consumers make decisions and propose how that change impacts services marketing, service research and service management.

Design/methodology/approach
A review of the literature, documentation of sales and customer service experiences support the evolution of bot-driven consumer decision-making, proposing the bot-driven service platform as a key component of the service experience.

Findings
Today the focus is on convenience, the less time and effort, the better. The authors propose that AI has taken convenience to a new level for consumers. By using bots as their service of choice, consumers outsource their decisions to algorithms, hence give little attention to traditional consumer decision-making models and brand emphasis. At the moment, this is especially true for low involvement types of decisions, but high involvement decisions are on the cusp of delegating to AI. Therefore, management needs to change how they view consumers’ decision-making-processes and how services are being managed.

Research limitations/implications
In an AI-convenience driven service economy, the emphasis needs to be on search ranking or warehouse stock, rather than the traditional drivers of brand values such as service quality. Customer experience management will shift from interaction with products and services toward interactions with new service platforms such as AI, bots. Hence, service marketing, as the authors know it might be in decline and be replaced by an efficient complex attribute computer decision-making model.

Originality/value
The change in consumer behavior leads to a change in the service marketing approach needed in the world of AI. The bot, the new service platform is now in charge of search and choice for many purchase situations.
Sustainability intention: mediator of sustainability behavioral control and sustainable entrepreneurship
Authors: Kimuli Saadat Nakyejwe Lubowa , Orobia Laura , Sabi Humphrey Muki , Tsuma Clive Katiba 
Published: April 2020

PurposeThe purpose of this paper is to report results of a study carried out to establish the mediation effect of sustainability intention in the relationship between sustainability behavioral control and sustainable entrepreneurship.Design/methodology/approachThis study is cross sectional and correlational. Data were collected through a questionnaire survey of 384 small businesses. Data were analyzed through correlation coefficients and linear regression using Statistical Package for Social Sciences and the Medgraph program.FindingsThe results suggest that sustainability intention partially mediates the relationship between sustainability behavioral control and sustainable entrepreneurship. Results further indicate that sustainability behavioral control and sustainability intention are significant predictors of sustainable entrepreneurship.Originality/valueThis study provides an initial empirical evidence on the mediation effect of sustainability intention in the relationship between sustainability behavioral control and sustainable entrepreneurship. To the researcher's knowledge, no study had been conducted on such an interesting topic using evidence from a developing country such as Uganda.
Sex‐specific differences in the response of prey to predation risk
Authors: Donelan Sarah C. , Trussell Geoffrey C. 
Published: April 2020

Abstract

The non‐consumptive effects of predation risk can strongly affect prey behaviour and fitness with emergent effects on community structure and ecosystem functioning. Prey may respond differently to predation risk based on key traits such as sex, but the influence of sex‐specific variation is typically explored in species with strong sexual dimorphism. However, sex‐specific responses to predation risk may arise even in prey species lacking sexual dimorphisms based on differences in the relative cost of reproduction.

Using a rocky intertidal food chain, we conducted a laboratory mesocosm experiment to explore sex‐specific responses of morphologically similar, reproductively mature prey (the snail Nucella lapillus) to predation risk and whether risk affected female fecundity.

We found that predation risk suppressed prey growth only in males via effects on growth efficiency, suggesting that sex‐specific disparities may arise due to differences in the energy required for reproduction and/or the costs of mounting a physiological stress response. Moreover, while risk did not affect overall female fecundity, it eliminated the positive relationship between female size and fecundity observed in the absence of risk.

We hypothesize that these sex‐specific disparities arise due to differences in the energy required for reproduction and/or the costs of mounting a physiological stress response. Reproduction is likely more energetically costly for females than males, so females may display weaker antipredator responses in order to maintain energetic reserves needed for reproduction. Our results suggest that sex‐specific responses may be an important component of inter‐individual differences in prey responses to risk and influence prey population growth and demography even in species lacking sexual dimorphism.

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