bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Wnt-mediated transformation of the bone marrow stromal cell identity orchestrates skeletal regeneration
Authors: Matsushita Yuki , Nagata Mizuki , Kozloff Kenneth M. , Welch Joshua D. , Mizuhashi Koji , Tokavanich Nicha , Hallett Shawn A. , Link Daniel C. , Nagasawa Takashi , Ono Wanida , Ono Noriaki 
Published: January 2020

AbstractBone marrow stromal cells (BMSCs) are versatile mesenchymal cell populations underpinning the major functions of the skeleton, a majority of which adjoin sinusoidal blood vessels and express C-X-C motif chemokine ligand 12 (CXCL12). However, how these cells are activated during regeneration and facilitate osteogenesis remains largely unknown. Cell-lineage analysis using Cxcl12-creER mice reveals that quiescent Cxcl12-creER+ perisinusoidal BMSCs differentiate into cortical bone osteoblasts solely during regeneration. A combined single cell RNA-seq analysis demonstrate that these cells convert their identity into a skeletal stem cell-like state in response to injury, associated with upregulation of osteoblast-signature genes and activation of canonical Wnt signaling components along the single-cell trajectory. β-catenin deficiency in these cells indeed causes insufficiency in cortical bone regeneration. Therefore, quiescent Cxcl12-creER+ BMSCs transform into osteoblast precursor cells in a manner mediated by canonical Wnt signaling, highlighting a unique mechanism by which dormant stromal cells are enlisted for skeletal regeneration.
Cooperative excitonic quantum ensemble in perovskite-assembly superlattice microcavities
Authors: Zhou Chun , Zhong Yichi , Dong Hongxing , Zheng Weihao , Tan Jiqing , Jie Qi , Pan Anlian , Zhang Long , Xie Wei 
Published: January 2020

AbstractPerovskites—compounds with the CaTiO3-type crystal structure—show outstanding performance in photovoltaics and multiparameter optical emitters due to their large oscillator strength, strong solar absorption, and excellent charge-transport properties. However, the ability to realize and control many-body quantum states in perovskites, which would extend their application from classical optoelectronic materials to ultrafast quantum operation, remains an open research topic. Here, we generate a cooperative quantum state of excitons in a quantum dot ensemble based on a lead halide perovskite, and we control the ultrafast radiation of excitonic quantum ensembles by introducing optical microcavites. The stimulated radiation of excitonic quantum ensemble in a superlattice microcavity is demonstrated to not be limited by the classical population-inversion condition, leading to a picosecond radiative duration time to dissipate all of the in-phase dipoles. Such a perovskite-assembly superlattice microcavity with a tunable radiation rate promises potential applications in ultrafast, photoelectric-compatible quantum processors.
The epichaperome is a mediator of toxic hippocampal stress and leads to protein connectivity-based dysfunction
Authors: Inda Maria Carmen , Joshi Suhasini , Wang Tai , Bolaender Alexander , Gandu Srinivasa , Koren III John , Che Alicia Yue , Taldone Tony , Yan Pengrong , Sun Weilin , Uddin Mohammad , Panchal Palak , Riolo Matthew , Shah Smit , Barlas Afsar , Xu Ke , Chan Lon Yin L. , Gruzinova Alexandra , Kishinevsky Sarah , Studer Lorenz , Fossati Valentina , Noggle Scott A. , White Julie R. , de Stanchina Elisa , Sequeira Sonia , Anthoney Kyle H. , Steele John W. , Manova-Todorova Katia , Patil Sujata , Dunphy Mark P. , Pillarsetty NagaVaraKishore , Pereira Ana C. , Erdjument-Bromage Hediye , Neubert Thomas A. , Rodina Anna , Ginsberg Stephen D. , De Marco Garcia Natalia , Luo Wenjie , Chiosis Gabriela 
Published: January 2020

AbstractOptimal functioning of neuronal networks is critical to the complex cognitive processes of memory and executive function that deteriorate in Alzheimer’s disease (AD). Here we use cellular and animal models as well as human biospecimens to show that AD-related stressors mediate global disturbances in dynamic intra- and inter-neuronal networks through pathologic rewiring of the chaperome system into epichaperomes. These structures provide the backbone upon which proteome-wide connectivity, and in turn, protein networks become disturbed and ultimately dysfunctional. We introduce the term protein connectivity-based dysfunction (PCBD) to define this mechanism. Among most sensitive to PCBD are pathways with key roles in synaptic plasticity. We show at cellular and target organ levels that network connectivity and functional imbalances revert to normal levels upon epichaperome inhibition. In conclusion, we provide proof-of-principle to propose AD is a PCBDopathy, a disease of proteome-wide connectivity defects mediated by maladaptive epichaperomes.
Genomic programming of IRF4-expressing human Langerhans cells
Authors: Sirvent Sofia , Vallejo Andres F. , Davies James , Clayton Kalum , Wu Zhiguo , Woo Jeongmin , Riddell Jeremy , Chaudhri Virendra K. , Stumpf Patrick , Nazlamova Liliya Angelova , Wheway Gabrielle , Rose-Zerilli Matthew , West Jonathan , Pujato Mario , Chen Xiaoting , Woelk Christopher H. , MacArthur Ben , Ardern-Jones Michael , Friedmann Peter S. , Weirauch Matthew T. , Singh Harinder , Polak Marta E. 
Published: January 2020

AbstractLangerhans cells (LC) can prime tolerogenic as well as immunogenic responses in skin, but the genomic states and transcription factors (TF) regulating these context-specific responses are unclear. Bulk and single-cell transcriptional profiling demonstrates that human migratory LCs are robustly programmed for MHC-I and MHC-II antigen presentation. Chromatin analysis reveals enrichment of ETS-IRF and AP1-IRF composite regulatory elements in antigen-presentation genes, coinciding with expression of the TFs, PU.1, IRF4 and BATF3 but not IRF8. Migration of LCs from the epidermis is accompanied by upregulation of IRF4, antigen processing components and co-stimulatory molecules. TNF stimulation augments LC cross-presentation while attenuating IRF4 expression. CRISPR-mediated editing reveals IRF4 to positively regulate the LC activation programme, but repress NF2EL2 and NF-kB pathway genes that promote responsiveness to oxidative stress and inflammatory cytokines. Thus, IRF4-dependent genomic programming of human migratory LCs appears to enable LC maturation while attenuating excessive inflammatory and immunogenic responses in the epidermis.
Neural circuits underlying auditory contrast gain control and their perceptual implications
Authors: Lohse Michael , Bajo Victoria M. , King Andrew J. , Willmore Ben D. B. 
Published: January 2020

AbstractNeural adaptation enables sensory information to be represented optimally in the brain despite large fluctuations over time in the statistics of the environment. Auditory contrast gain control represents an important example, which is thought to arise primarily from cortical processing. Here we show that neurons in the auditory thalamus and midbrain of mice show robust contrast gain control, and that this is implemented independently of cortical activity. Although neurons at each level exhibit contrast gain control to similar degrees, adaptation time constants become longer at later stages of the processing hierarchy, resulting in progressively more stable representations. We also show that auditory discrimination thresholds in human listeners compensate for changes in contrast, and that the strength of this perceptual adaptation can be predicted from physiological measurements. Contrast adaptation is therefore a robust property of both the subcortical and cortical auditory system and accounts for the short-term adaptability of perceptual judgments.
United States wildlife and wildlife product imports from 2000–2014
Authors: Eskew Evan A. , White Allison M. , Ross Noam , Smith Kristine M. , Smith Katherine F. , Rodríguez Jon Paul , Zambrana-Torrelio Carlos , Karesh William B. , Daszak Peter 
Published: January 2020

AbstractThe global wildlife trade network is a massive system that has been shown to threaten biodiversity, introduce non-native species and pathogens, and cause chronic animal welfare concerns. Despite its scale and impact, comprehensive characterization of the global wildlife trade is hampered by data that are limited in their temporal or taxonomic scope and detail. To help fill this gap, we present data on 15 years of the importation of wildlife and their derived products into the United States (2000–2014), originally collected by the United States Fish and Wildlife Service. We curated and cleaned the data and added taxonomic information to improve data usability. These data include >2 million wildlife or wildlife product shipments, representing >60 biological classes and >3.2 billion live organisms. Further, the majority of species in the dataset are not currently reported on by CITES parties. These data will be broadly useful to both scientists and policymakers seeking to better understand the volume, sources, biological composition, and potential risks of the global wildlife trade.
Epidemiological profile of mucosal melanoma in Brazil
Authors: Cohen Goldemberg Daniel , de Melo Andreia Cristina , de Melo Pino Livia Cristina , Thuler Luiz Claudio Santos 
Published: January 2020

AbstractMucosal melanomas are primary malignant neoplasias originated from melanocytes within mucous membranes in any part of mucosal surface lining, more commonly, in the nasal cavity and accessory sinuses, oral cavity, lips, pharynx, vulvar, vaginal, cervix and anorectal mucosa. Epidemiology data regarding mucosal melanomas in Brazil is scarce, hence the motivation to conduct this research paper. The χ2 test was used to compare categorical variables. Forward stepwise logistic regression method was used in the multivariate analysis to identify independent predictors of early death. A total of 801 patients were included in the analysis. Surgical resection is frequently the first approach to primary tumours (65.3%), even though the utility of lymph node surgery and radiation therapy is not well established. Advanced stage was observed in more than two thirds of patients. Early death was observed in 28.3%. MM cases with regional or distant metastases as well as those located in unusual locations had almost 4 times more risk for early death. Besides that, MM located in lips, oral cavity and pharynx and those receiving chemotherapy had 2 times more risk of early death.
Identification of plasmalogens in Bifidobacterium longum, but not in Bifidobacterium animalis
Authors: Mawatari Shiro , Sasuga Yasuhiro , Morisaki Tomomi , Okubo Mika , Emura Takako , Fujino Takehiko 
Published: January 2020

AbstractPlasmalogens are glycerophospholipids that contain a vinyl ether bond at the sn-1 position of glycerol backbone instead of an ester bond. Plasmalogens are indicated to have many important functions in mammalian cells. On the other hand, it is suggested that some gut microbiota plays many probiotic functions to human health. Presence of plasmalogens in Clostridium strains in gut microbiota is well-known, but presence of plasmalogens in Bifidobacterium longum (B. longum) strain, one of the most important probiotic gut microbiota, has not been reported. We identified plasmalogens in lipid extract from some B. longum species, but not from Bifidobacterium animalis (B. animalis) species which are another important strain of probiotic bifidobacteria. Major phospholipid classes of plasmalogens in B. longum species were cardiolipin, phosphatidylglycerol and phosphatidic acid. Almost all of the phospholipids from B. longum examined were indicated to be plasmalogens. Although major phospholipid classes of plasmalogens in human brain and major phospholipid classes of plasmalogens in B. longum are different, it is interesting to note that many reported functions of microbiota-gut-brain axis on human neurodegenerative diseases and those functions of plasmalogens on neurodegenerative diseases are overlapped. The presence of plasmalogens in B. longum species may play important roles for many probiotic effects of B. longum to human health.
Induction of E6AP by microRNA-302c dysregulation inhibits TGF-β-dependent fibrogenesis in hepatic stellate cells
Authors: Kim Ji Young , Kim Kyu Min , Yang Ji Hye , Cho Sam Seok , Kim Seung Jung , Park Su Jung , Ahn Sang‐Gun , Lee Gum Hwa , Yang Jin Won , Lim Sung Chul , Kang Keon Wook , Ki Sung Hwan 
Published: January 2020

AbstractHepatic stellate cells (HSCs) are essential for liver fibrosis. E6 associated protein (E6AP) is one of the E3-ubiquitin-protein ligase and has been studied in proliferation and cellular stress. Currently, no information is available on the role of E6AP on transforming growth factor-β (TGF-β) signaling and hepatic fibrogenesis. This study examined whether E6AP is overexpressed in activated HSCs, and if so, its effect on hepatic fibrogenesis and the molecular mechanism. E6AP was expressed higher in HSCs than hepatocytes, and was up-regulated in activated HSCs, HSCs from the livers of carbon tetrachloride-injected mice, or TGF-β-treated LX-2 cells. The TGF-β-mediated E6AP up-regulation was not due to altered mRNA level nor protein stability. Thus, we performed microRNA (miRNA, miR) analysis and found that miR-302c was dysregulated in TGF-β-treated LX-2 cells or activated primary HSCs. We revealed that miR-302c was a modulator of E6AP. E6AP overexpression inhibited TGF-β-induced expression of plasminogen activator inhibitor-1 in LX-2 cells, albeit it was independent of Smad pathway. Additionally, E6AP inhibited TGF-β-mediated phosphorylation of mitogen-activated protein kinases. To conclude, E6AP overexpression due to decreased miR-302c in HSCs attenuated hepatic fibrogenesis through inhibition of the TGF-β-induced mitogen-activated protein kinase signaling pathway, implying that E6AP and other molecules may contribute to protection against liver fibrosis.
Incidence of breast cancer attributable to breast density, modifiable and non-modifiable breast cancer risk factors in Singapore
Authors: Ho Peh Joo , Lau Hannah Si Hui , Ho Weang Kee , Wong Fuh Yong , Yang Qian , Tan Ken Wei , Tan Min-Han , Chay Wen Yee , Chia Kee Seng , Hartman Mikael , Li Jingmei 
Published: January 2020

AbstractIncidence of breast cancer is rising rapidly in Asia. Some breast cancer risk factors are modifiable. We examined the impact of known breast cancer risk factors, including body mass index (BMI), reproductive and hormonal risk factors, and breast density on the incidence of breast cancer, in Singapore. The study population was a population-based prospective trial of screening mammography - Singapore Breast Cancer Screening Project. Population attributable risk and absolute risks of breast cancer due to various risk factors were calculated. Among 28,130 women, 474 women (1.7%) developed breast cancer. The population attributable risk was highest for ethnicity (49.4%) and lowest for family history of breast cancer (3.8%). The proportion of breast cancers that is attributable to modifiable risk factor BMI was 16.2%. The proportion of breast cancers that is attributable to reproductive risk factors were low; 9.2% for age at menarche and 4.2% for number of live births. Up to 45.9% of all breast cancers could be avoided if all women had breast density <12% and BMI <25 kg/m2. Notably, sixty percent of women with the lowest risk based on non-modifiable risk factors will never reach the risk level recommended for mammography screening. A combination of easily assessable breast cancer risk factors can help to identify women at high risk of developing breast cancer for targeted screening. A large number of high-risk women could benefit from risk-reduction and risk stratification strategies.
An Experimental Investigation of Ultraweak Photon Emission from Adult Murine Neural Stem Cells
Authors: Esmaeilpour Tahereh , Fereydouni Esmaeil , Dehghani Farzaneh , Bókkon Istvan , Panjehshahin Mohammad-Reza , Császár-Nagy Noemi , Ranjbar Mehdi , Salari Vahid 
Published: January 2020

AbstractNeurons like other living cells may have ultraweak photon emission (UPE) during neuronal activity. This study is aimed to evaluate UPE from neural stem cells (NSC) during their serial passaging and differentiation. We also investigate whether the addition of silver nanoparticles (AgNPs) or enhancement of UPE (by AgNPs or mirror) affect the differentiation of NSC. In our method, neural stem and progenitor cells of subventricular zone (SVZ) are isolated and expanded using the neurosphere assay. The obtained dissociated cells allocated and cultivated into three groups: groups: I: cell (control), II: cell + mirror, and III: cell + AgNPs. After seven days, the primary neurospheres were counted and their mean number was obtained. Serial passages continuous up to sixth passages in the control group. Differentiation capacity of the resulting neurospheres were evaluated in vitro by immunocytochemistry techniques. Measurement of UPE was carried out by photomultiplier tube (PMT) in the following steps: at the end of primary culture, six serial cell passages of the control group, before and after of the differentiation for 5 minutes. The results show that neither mirror nor AgNPs affect on the neurosphere number. The UPE of the NSC in the sixth subculturing passage was significantly higher than in the primary passage (P < 0.05). AgNPs significantly increased the UPE of the NSC compared to the control group before and after the differentiation (P < 0.05). Also, the treatment with AgNPs increased 44% neuronal differentiation of the harvested NSCs. UPE of NSC after the differentiation was significantly lower than that before the differentiation in each groups, which is in appropriate to the cell numbers (P < 0.0001). The mirror did not significantly increase UPE, neither before nor after the differentiation of NSC. As a conclusion, NSC have UPE-properties and the intensity is increased by serial passaging that are significant in the sixth passage. The AgNPs increases the UPE intensity of NSC that pushes more differentiation of NSC to the neurons. The mirror was not effective in enhancement of UPE. As a result, UPE measurement may be suitable for assessing and studying the effects of nanoparticles in living cells and neurons.
Convergence of physiological responses to pain during face-to-face interaction
Authors: Murata Aiko , Nishida Hiroshi , Watanabe Katsumi , Kameda Tatsuya 
Published: January 2020

AbstractEmpathy with another’s pain is an important social glue for maintaining interpersonal relationships. In most previous studies investigating the sharing of pain, a signal conveying a painful experience is presented by a target (“sender”) as a stimulus to a participant (“receiver”), and the emotional/physiological responses of the participant are measured. However, this unilateral “sender-receiver” paradigm does not adequately address the possible bidirectional experience of shared pain accruing from interaction. Our aim was therefore to investigate the bidirectional effects of sharing pain in social settings. Thirty-six unfamiliar pairs were simultaneously and repeatedly exposed to the same pain-provoking (thermal) stimuli, either in a face-to-face or a “shielded” condition where a partition prevented the partner’s responses from being fully observed. We recorded the blood volume pulse of each participant to measure the acute sympathetic response while a pair of participants experienced the stimuli simultaneously. The results revealed that participants with weaker reactions elevated their physiological reactivity to the stimulus in accordance with their partner’s reactions in the face-to-face condition. The pair-level physiological similarity was also higher compared to the shielded condition. Such a low-to-high physiological convergence may underlie the collective elevation of pain expressions, which is often observed in interactive settings.
Population based Air Pollution Exposure and its influence factors by Integrating Air Dispersion Modeling with GIS Spatial Analysis
Authors: Dong Xiaoya , Zhao Xiuge , Peng Fen , Wang Danlu 
Published: January 2020

AbstractAir pollution is a major environmental health problem. The study of interaction between air pollution and human will benefit to the human health and well-being of community. Both a model for assessing population relative risk of air pollution exposure (MAPRRAPE) and air pollution concentration methods were applied in a case study to determine the optimal method in evaluating risk of population exposure to Sulfur Dioxide (SO2). The framework for building the MAPRRAPE was described in detail. Then, the spatial patterns of population by demographic characteristics exposed to SO2from industrial, vehicle, and the mixture of industrial and vehicle pollution sources, as well as an in-depth quantitative investigation using correlation analysis were studied for further source appointment. The results showed that the MAPRRAPE was more reliable than air pollution concentration model in determining population exposure risks by demographic characteristics. The high risk areas of whites exposed to SO2were larger than blacks and the other races due to a large number of whites, and other age groups exposed to SO2were larger than children and the old people. In addition, the correlation analyses showed that the relative risks of population by demographic characteristics exposed to SO2had a more significant correlation with vehicle pollution source than industrial pollution source. The results of source appointment thus demonstrated that vehicle pollution source was the main pollution source. This study suggests that there is a clear need for the implementation of programs and services that will reduce population exposed to air pollution with focusing on densely populated areas for an ultimate improvement of community health status and the environmental conditions.
A Novel Biosorbent for Preconcentrations of Co(II) and Hg(II) in Real Samples
Authors: Ozdemir Sadin , Kılınç Ersin , Fatih Sen 
Published: January 2020

AbstractA new biosorbent, composed of Amberlite XAD-4 loaded with Anoxybacillus kestanboliensis, was developed and surface morphologies were investigated by SEM and FT-IR. It was used for solid phase column preconcentrations of Co(II) and Hg(II) before their measurements by ICP-OES. LODs were calculated as 0.04 and 0.06 ng mL−1 for Co(II) and Hg(II) respectively. The maximum biosorption capacities were determined as 24.3 and 27.8 mg g−1 for Co(II) and Hg(II) respectively. Preconcentration factors were achieved for Co(II) and Hg(II) as 80. The method validation was performed by analyzing certified reference materials. The new process was successfully utilized for the preconcentration of these metals in various food samples. It should be highlighted that the sensitivity of ICP-OES was critically improved by applying developed method. Hence, ICP-OES could be an effective alternative for ICP-MS and/or GF-AAS.