bluuebunny/crossref_metadata_2025_split huggingface dataset:
The impact of COVID-19 epidemic on adult Myasthenia Gravis patients
Authors: Peng Yuyao , Li Zhibin , Zeng Qiuming , Yang Huan 
Published: April 2020

Abstract
Objects: To investigate the impact of COVID-19 epidemic on adult myasthenia gravis (MG) patients, in order to provide better medical advice for special patient population during the quarantine period.Methods: Adult MG patients were randomly recruited to participate. The survey consisted of self-designed questionnaires and the revised 15-item Myasthenia Gravis Quality of Life Questionnaire (MGQOL15r). Participation was strictly voluntary and remained anonymous.Results: A total of 214 adult MG patients (84 males and 130 females) gave valid replies. There were 49.53% patients reported they had the fear of COVID-19 outbreak at different levels. And female patients had a significant higher level of fear to COVID outbreak (p=0.009). It was found that the adult MG patients are more likely to be influenced by the COVID-19 epidemic compared to the general population. During the COVID-19 outbreak, 36.45% patients reported they got an improvement of MG disease severity. 57.01% patients reported they felt no change. 6.54% patients reported their condition got worse. The average of MGQOL15r score was 7.38±6.22. The higher level of the fear of COVID-19 outbreak, the higher score of MGQOL15r (p=0.029), the poorer quality of life. 96.73% patients hoped the specialist physicians could provide online consult. In addition, 64.95% patients would go to the tertiary hospital without hesitation if the Outpatients Department was opened even during the COVID-19 epidemic.Conclusions: The COVID-19 epidemic has significant impact on the quality of life and psychological status in the adult MG patients. Compared to the general population, the adult MG patients are more likely to be influenced by the COVID-19 epidemic. Hence, it is important for health care organizations to provide professional therapeutic advice and psychosocial support in time.*, Contributed to this work equally.
Multicenter randomized controlled trial comparing digital and traditional chest drain in a VATS pulmonary lobectomy cohort: interim analysis
Authors: Mendogni Paolo , Tosi Davide , Marulli Giuseppe , Comacchio Giovanni Maria , Pieropan Sara , Rossi Veronica , Brascia Debora , Andriolo Luigi Gaetano , Imbriglio Giovanna , Bonitta Gianluca , Lopez Camillo , Rea Federico , Nosotti Mario 
Published: April 2020

Abstract
Background The usefulness of digital chest drain is still discussed. We are carrying out a study to determine if the use of a digital system compared with a traditional system reduces the duration of chest drainage. To evaluate safety, benefit, or futility of this trial we planned the current interim analysis. Methods An interim analysis on preliminary data from ongoing investigator-initiated, multicenter, interventional, prospective randomized trial. Original protocol number: (NCT03536130). The interim main endpoint was overall complications; secondary endpoints were the concordance between the two primary endpoints of the RCT (chest tube duration and length of hospital stay). We planned the interim analysis when half of the patients have been randomised and completed the study. Data were described using mean and standard deviation or absolute frequencies and percentage. T-test for unpaired samples, Chi-square test, Poisson regression and absolute standardized mean difference (ASMD) were used. P-value <0.05 was considered significant. Results From April 2017 to November 2018, out of 317 patients were enrolled by 3 centers, 231 fulfilled inclusion criteria and were randomized. Twenty-two of them dropped out after randomization. Finally, 209 patients were analyzed: among them 94 used the digital device and 115 the traditional one. The overall postoperative complications were 35 (16.8%) including prolonged air leak (1.9%). Mean chest tube duration was 3.6 days (SD=1.8), with no differences between two groups (p=0.203). The overall difference between hospital stay and chest tube duration was 1.4 days (SD=1.4). Air leak at first postoperative day detected by digital and traditional devices predicted increasing in tube duration of 1.6 day (CI 95% 0.8-2.5, p<0.001) and 2.0 days (CI 95% 1.0-3.1, p<0.001), respectively. Conclusions This interim analysis supported the authors’ will to continue with the enrollment and to analyze data once the estimated sample size will be reached.
Investigating the healthcare pathway through patient’s experience and profiles: implications for the breast cancer healthcare providers
Authors: Cherif Emna , Verdier Elisabeth Martin , Rochette Corinne 
Published: April 2020

Abstract
BackgroundHealthcare systems are facing many changes. Particularly, patients are more engaged in the care process. The medical perspective of the process is insufficient to provide patients with high care quality and service personalization. This research presents an attempt to complete this medical perspective through an experiential perspective, especially for chronic diseases such as cancer. We investigated patients’ experiences and profiles to reach a deeper understanding of their needs and expectations when they confront the disease.The objectives of this research were to model the key stages underling the patient pathway and to identify the challenging touchpoints of the interactions between the patients and the healthcare providers. Bringing together findings of patient experience, pathway, and profiles would help all the stakeholders involved to develop better practices for the healthcare process.MethodsA qualitative observational nethnography on a French specialized forum for breast cancer patients “les Impatientes” was conducted. A total of 967 reviews were collected over a complete year period from all over France. Thematic and lexicometric content analysis were performed according to the experience dimensions, the pathway stages and touchpoints, as well as the patients’ profiles.ResultsData analysis shows that the healthcare pathway experienced by the patients is built around three stages. The discovery stage is closely related to the emotional dimension regarding the patient and physician relationship. The examination stage is characterized by a more technical and informational needs for the types of treatments. The follow-up and control stage illustrates the patients’ need to assess the treatments’ effectiveness and the quality of the follow-up. Moreover, three profiles of patients were identified. The newcomers, the altruists and the autonomous are characterized by different attitudes depending on the stage of the healthcare pathway they are living.ConclusionsOur research presents an original modelling of the patient pathway and profiles beyond the medical process. It gives practical tracks to improve of the healthcare pathway. Patients expect healthcare providers to integrate and strengthen several challenging touchpoints in order to create satisfactory patient experiences and high service quality.
FRET-based Tau seeding assay does not represent prion-like templated assembly of Tau filaments
Authors: Kaniyappan Senthilvelrajan , Tepper Katharina , Biernat Jacek , Chandupatla RamReddy , Hübschmann Sabrina , Irsen Stephan , Bicher Sandra , Klatt Christoph , Mandelkow Eva-Maria , Mandelkow Eckhard 
Published: April 2020

Abstract
Tau aggregation into amyloid fibers based on the cross-beta structure is a hallmark of several Tauopathies, including Alzheimer Disease (AD). Trans-cellular propagation of Tau with pathological conformation has been suggested as a key disease mechanism. This is thought to cause the spreading of Tau pathology in AD by templated conversion of naive Tau in recipient cells into a pathological state, followed by assembly of pathological Tau fibers, similar to the mechanism proposed for prion pathogenesis. In cell cultures, the process is usually monitored by a FRET assay where the recipient cell expresses the Tau repeat domain (Tau RD ) with a pro-aggregant mutation, fused to GFP-based FRET pairs. Since the diameter of the reporter GFP (~3 nm) is ~6.5 times larger than the β-strand distance (0.47nm), this points to a potential steric clash. Hence, we investigated the influence of the GFP tag on Tau aggregation. Using biophysical methods (light scattering, atomic force microscopy (AFM), and scanning-transmission electron microscopy (STEM)), we found that the assembly of Tau-GFP was severely inhibited and incompatible with that of Alzheimer filaments. These observations argue against the hypothesis that the propagation of Tau pathology in AD is caused by the prion-like templated aggregation of Tau protein, transmitted via cell-to-cell spreading of Tau. As a consequence, the observed local increase of FRET in recipient cells must be caused by alternative processes.
The Effects of Mechanical Insufflation-Exsufflation on Lung Function and Complications in Cardiac Surgery Patients
Authors: Wu Meng-Fang , Wang Tsai-Yu , Chen Da-Shen , Shiao Shou-Fong , Hu Han-Chuang , Chung Fu-Tsai , Lin Ting-Yu , Lin Shu-Min 
Published: April 2020

Abstract
Background: Postoperative positive pressure lung expansion is associated with decreased pulmonary complications and improved clinical outcomes. The aim of the present study was to compare the differences in post-operative pulmonary complications and clinical outcomes between two groups of study subjects who underwent cardiac surgery; one included subjects who received mechanical insufflation-exsufflation (MI-E) and the other included subjects who received intermittent positive pressure breathing (IPPB) therapy.Methods: This retrospective study included 48 subjects, who underwent cardiac surgery in an intensive care unit of a tertiary hospital during the time period from June 2017 to February 2018. After liberation from mechanical ventilation, the subjects received lung expansion therapy by means of two type of positive pressure devices, MI-E (n=20) or IPPB (n=28). The pulmonary complications, lung function, and clinical outcomes were compared between the two groups.Results: Subjects in both groups displayed similar baseline characteristics and underwent similar types of surgical procedures. Compared to subjects who received non-oscillatory therapy, those who received MI-E therapy had higher post-operative force vital capacity (58.9±4.96 % vs. 45.21±3.60 %, p=0.026), forced expiratory volume in one second (63.35±5.4 % vs. 45.48±3.63 %, p=0.007), and peak flow rate (68.5±5.53 % vs. 54.75±4.11 %, p=0.047). However, the incidence of chest pain was higher in the MI-E group (n=13, 65%) than in the IPPB group (n=4, 14.3%; odds ratio, 11.14, 95% confidence interval, 2.74-45.26; p=0.001). The length of hospital and ICU stay, development of atelectasis, pneumonia, and pleural effusion were similar in both the groups.Conclusion: Both IPPB and MI-E therapies have similar effects on preventing post-operative complications in cardiac surgery patients. However, compared to IPPB therapy, MI-E therapy was associated with improved pulmonary function and higher incidence of chest pain.
Potential Molecular Mechanism of the NPPB Gene in Post-Ischemic Heart Failure with and without T2DM
Authors: Guan Yao-Zong , Yin Rui-Xing , Deng Guo-Xiong , Zheng Peng-Fei , Liu Chun-Xiao , Wei Bi-Liu 
Published: April 2020

Abstract
Background: This study aimed to investigate natriuretic peptide B (NPPB) co-expression genes and the pathways involved in post-ischemic heart failure (HF) among patients both with and without type 2 diabetes mellitus (T2DM). Methods: The microarray dataset of GSE26887 was examined to detect the genes that co-expressed with NPPB from 19 post-ischemic HF patients’ peripheral blood samples (7 with T2DM and 12 without T2DM). NPPB co-expression genes were then screened using the R packet. Further, using online analytical tools, we determined the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis, Gene Ontology (GO) annotation, and protein-protein interaction (PPI) network of the co-expression genes. The modules and hub genes of the PPI network were then identified using the Cytoscape software. Results: In patients with T2DM, a total of 41 biological processes (BP), 20 cellular components (CC), 13 molecular functions (MF), and 41 pathways were identified. Further, a total of 61 BPs, 16 CCs, 13 MFs, and 22 pathways in patients without T2DM were identified. In both groups of patients, 17 BPs, 10 CCs, 6 MFs, and 13 pathways were enriched. We also identified 173 intersectional co-expression genes (63 positive, 106 negative, and 4 differently co-expressed in patients with and without T2DM, respectively) in both types of patients, which enriched in 16 BPs, 8 CCs, 3 MFs, and 8 KEGG pathways. Moreover, the PPI network (contained 237 edges and 170 nodes) with the top module significantly enriched in 4 BPs (the tricarboxylic acid metabolic process, citrate metabolic process, tricarboxylic acid cycle, and aerobic respiration) and 3 pathways (the citrate cycle, malaria parasite metabolic pathway, and AGE-RAGE signaling pathway in diabetic complications) was constructed. DECR1, BGN, TIMP1, VCAN and CTCF are the top hub genes. Conclusions: This study used genome-wide co-expression genes to identify the potential functions and mechanisms of the NPPB gene in post-ischemic HF with and without T2DM. Our findings may elucidate the functions and roles of NPPB in patients with post-ischemic HF and facilitate HF management. Key words: Co-expression genes, NPPB, Heart failure, Diabetes mellitus, Microarray dataset
Identification of an Immune-Related Prognostic Signature Associated with Immune Infiltration in Melanoma
Authors: Liu Nian , Liu Zijian , Liu Xinxin , Duan Xiaoru , Huang Yuqiong , Jin Zilin , Zhang Liling , Chen Hongxiang 
Published: April 2020

Abstract
Background: Melanoma is the leading cause of cancer-related death among skin tumors, with an increasing incidence worldwide. Few studies have effectively investigated the significance of an immune-related genes (IRGs) signature for melanoma prognosis. Methods: Here, we constructed an IRGs prognostic signature using bioinformatics methods and evaluated and validated its predictive capability. Then, immune cell infiltration and tumor mutation burden (TMB) landscapes associated with this signature in melanoma were analyzed comprehensively. Results: With the 10-IRG prognostic signature, melanoma patients in the low-risk group showed better survival with distinct features of high immune cell infiltration and TMB. Importantly, melanoma patients in this subgroup were significantly responsive to MAGE-A3 in the validation cohort. Conclusions: This immune-related prognostic signature is thus a reliable tool to predict melanoma prognosis; as the underlying mechanism of this signature is associated with immune infiltration and mutation burden, it might reflect the benefit of immunotherapy to patients.
Assessing longitudinal and cross-sectional effects of age on adult obesity in an Iranian population: results from a large population-based cohort study
Authors: Alhossaini Mahsa Rafiee , Kazemnejad Anoshirvan , Zayeri Farid , Sadeghi Masoumeh 
Published: April 2020

Abstract
BackgroundObesity is a significant risk factor for Noncommunicable diseases, and it is related to many adverse health consequences. The risk of obesity commonly changes with age, which is called a longitudinal or aging effect. Also, individuals born or enter to the study of the same age have similar living conditions that may influence their obesity risk in a particular way; this is a cross-sectional effect. In the current study, an advanced statistical model is used to distinguish between longitudinal and cross-sectional effects of age on the risk of obesity for men and women.MethodsParticipants are a group of 6504 Iranian adults over 35 years of age in 2001, who live in the central region of Iran. They were followed up for 12 years in a large community-based study. Various medical indexes, including Body Mass Index, were collected in 2001, 2007, and 2013. The Marginal Logistic Regression model, which includes linear and quadratic effects of the Baseline Age and its difference with current age, is used.ResultsBetween 2001 and 2013, the prevalence of obesity raised from 13% to 18% in men and from 31% to 44% in women. The odds of obesity for women was approximately three times the odds of obesity for men on average adjusting for the age effects. Both cross-sectional and longitudinal effects of age were significantly associated with the odds ratio of obesity. There was a rise in the prevalence of obesity for individuals with Baseline Age 35 to 55 and a decline thereafter. Also, the odds ratio of obesity across one’s life course, had about 3% increase, on average, by each year aging, regardless of the age at baseline.ConclusionsThe high rate of obesity and its fast growth is a serious public health issue among Iranians, especially in adults age 35-55, and women. In the present study, Baseline Age was more strongly associated with the risk of obesity than aging. Considering both cross-sectional and longitudinal effects of age, helps us to understand the effect of age on obesity better and to identify the related factors.
Three-dimensional printing simulated operation combined with robot-aided minimally invasive lumbopelvic fixation in treatment of unstable bilateral sacral fractures: technical notes and 8 case series
Authors: liu Zhaojie , Tian Wei , Jin Xin , Qi Haotian , Sun Yuxi , Hu Yongcheng , Jia Jian 
Published: April 2020

Abstract
Background Despite their seldom appearance, unstable bilateral sacral fractures are severe injuries and always cause surgical management difficulties. Lumbopelvic fixation is reliable for rigid method, but wound-related complications with open procedure have been relatively common. Methods Data of 8 patients with unstable bilateral sacral fractures who were treated surgically in our institution from March 2016 to April 2019 were retrospectively analyzed. There were 5 men and 3 women with an average age of 38.5 years (range, 19-60years). According to the sharp of sacral fractures, there were one case with simple bilateral vertical fracture lines, 6 cases with “U” and one case with “H”. According to Roy-Camille classification, 7 of 8 sacral fractures involving sacral canal were classified with type I 2 cases, type II 4 cases and type III 1 case. Three-dimensional(3D) printing pelvis were performed to simulate lumbopelvic and sacroiliac screw fixation for preoperative planning. Eight bilateral sacral fractures were treated with minimally invasive lumbopelvic fixation under robotic guidance. Results The screws inserted with robotic assistance were exposed to radiation with an average of 41.6±10.2 times (range, 27–53 times) intraoperatively. The total fluoroscopy time was 32–59 s, and the average fluoroscopy time for each screw was 4.2±0.6s. According to modified Gras classification of screw position, there were Grade I in 7 cases and Grade II in one case (left S1 screw). The average operation time was 150.6 min (range, 95-220 min), and intraoperative blood loss was 87.5 ml (range, 60-120 ml). Eight patients were followed up consecutively for at least 12 months, with an average of 17.0 months (range, 12–24months). No patient suffered a neurovascular injury intraoperatively. There were no incision-related complications. All fractures healed with an average time of 4.2 months (range, 3–10 months). According to Majeed functional assessment investigation, the mean score was 88.4 points (range, 78–98 points), which were graded as follows: 5, excellent and 3, good. Conclusion Under robot guidance, minimally invasive lumbopelvic fixation for unstable bilateral sacral fractures is a feasible option with the advantages of accuracy, less radiation and safety. Simulated operation with 3D printing for preoperative planning can simplify the actual surgery.
Factors Related to Sexual Satisfaction in Women with Gestational Diabetes in the Third Trimester
Authors: zare ommolbanin , simbar masoumeh , ozgoli giti , bahar adaleh , majd hamid alavi 
Published: April 2020

Abstract
Background: Sexual satisfaction is important for maintaining pregnant women’s self-esteem and interpersonal relationships, and serves as a major indicator of health. Affecting a woman's sexual issues will indirectly affect her quality of life. The present study aimed to examine the factors related to sexual satisfaction in women with gestational diabetes in the third trimester. Method: This cross-sectional descriptive-analytic study was conducted in 2019 on 120 women with gestational diabetes visiting the clinics affiliated with Mazandaran University of Medical Sciences. Multistage random cluster sampling was performed, and the sample was selected in a non-probability and convenience manner. The data collection instruments included a demographic and midwifery-related information questionnaire, Hudson’s Index of Sexual Satisfaction, the Female Sexual Function Index, ENRICH Marital Satisfaction Scale (short version), the Female Sexual Distress Scale, The World Health Organization Quality of Life questionnaire, the Parental Distress Questionnaire, and the Depression, Anxiety, and Stress Scale. Data were analyzed in SPSS 23 by using descriptive statistics, chi-squared test, t-test, ANOVA, Pearson’s correlation coefficient, Kruskal-Wallis test, Mann-Whitney U test, and Spearman correlation coefficient. Results: Results showed that about 50% of women with gestational diabetes had low and moderate sexual satisfaction. In women with gestational diabetes, sexual satisfaction was positively correlated with sexual function (β = 0.215, p = 0.02) and marital satisfaction (β = 0.298, p = 0.003), and negatively correlated with depression (β = -0.269, p = 0.05). Conclusion: Half of the women with gestational diabetes had low and moderate sexual satisfaction. Marital satisfaction, sexual function, and depression significantly predicted sexual satisfaction in women with gestational diabetes. Examination of sexual function, marital satisfaction, and depression must be part of the routine evaluation for women with gestational diabetes. To increase the sexual satisfaction of these women, it is essential to offer interventions for reducing depression and improving sexual function, marital satisfaction and, thus, their quality of life. Keywords: Sexual satisfaction, gestational diabetes, pregnancy