bluuebunny/crossref_metadata_2025_split huggingface dataset:
Antibiotic Resistance Genes in Different Animal Manure and their Derived Organic Fertilizer
Authors: Xu Yan , Li Houyu , Shi Rongguang , Lv Jiapei , Li Bihan , Yang Fengxia , Zheng Xiangqun , Xu Jian 
Published: May 2020

Abstract
Background: The prevalence of antibiotic resistance genes (ARGs) in animal manure poses threats to the environmental safety. Organic fertilizers fermented by livestock and poultry manure are directly applied to farmland, which would cause the potential outbreak of bacterial resistance in agricultural environment. This study investigated the composition of ARGs in different animal manure and their derived organic fertilizers. Results: Results showed that the abundance of several ARGs, such as sul 2, Tet B-01, Tet G-01 and Tet M-01 in organic fertilizer samples was 12%~96% lower than in animal manure. However, there was an increasing of Tet K and erm C abundance from animal manure to the organic fertilizers. No correlation between ARGs and environmental factors such as pH, TN, antibiotics was observed by Redundancy analysis (RDA). Procrustes analysis revealed the significant correlation between bacterial community structures and the ARGs abundance (r=0.799, p<0.01). Non-metric multidimensional scaling (NMDS) analysis suggested that microorganisms in organic fertilizer may be derived from animal manure. Additional, pathogenic bacteria (especially Actinomadura ) would proliferate rather than decrease from manure to organic fertilizer. Conclusion: Overall, this research suggests that the composting treatment of manure could effectively reduce these ARGs and pathogens,even cause partial ARGs and pathogens proliferation. It also shows that the microorganism might significantly influence ARGs profiles in composting.
Food Groups Intake in Relation to Stunting Among Exceptional Children
Authors: Nachvak Seyyed Mostafa , Moradi Shima , Sadeghi Omid , Esmailzadeh Ahmad , Mostafai Roghayeh 
Published: May 2020

Abstract
Background Although several studies have examined the link between different food groups intake and stunting among children, no study, to our knowledge, was done on exceptional children. The aim of this study was assessed the association of dietary intake and stunting in Iranian exceptional children.Methods This cross-sectional study was conducted on 470 exceptional children (226 mentally retarded, 182 deaf and 62 blind children), aged 5–15 years. Height was measured using standard tool. Stunting was defined as height-for-age z-score of <-1. A validated dietary habit questionnaire was applied to assess dietary intakes.Results Mean age of children was 10.02 ± 2.04 years. Stunting was prevalent among 50.6% of children. Compared with children in the lowest category of dairy consumption, those in the highest category had lower odds of stunting. This association remained significant even after adjusting for covariates (OR: 0.50, 95% CI: 0.29–0.87). In addition, moderate consumption of egg (1–3 time/wk) was inversely associated with stunting either before or after controlling for potential confounders (OR: 0.36, 95% CI: 0.21–0.64). Such finding was also seen among mentally retarded children (OR: 0.38, 95% CI: 0.16–0.89). No other significant association was seen between intakes of other food groups (including meat, fruits and vegetables) and stunting.Conclusion We found that higher intake of dairy products and egg was associated with lower risk of stunting. However, intakes of other food groups including meat, fruits and vegetables were not significantly related to stunting.
Virulence factors and molecular characteristics of Shigella flexneri isolated from calves with diarrhea
Authors: Zhu Zhen , Wang Weiwei , Cao Mingze , Liu Guanhui , Zhou Xuzheng , Li Bing , Shi Yuxiang , Zhang Jiyu 
Published: May 2020

Abstract
Background: The natural hosts of Shigella are conventionally humans and other primates; however, the host range of Shigella has been shown to expand to many animals. Although Shigella is becoming a huge threat to animals, there is limited information on the genetic background of local strains. The purpose of this study was to assess the presence of virulence factors and the molecular characteristics of S. flexneri isolated from calves with diarrhea.Methods: From 2014 to 2016, 54 S. flexneri isolates were collected from diarrhea, and their biochemical characteristics were determined according to API20E and virulence factors via PCR. The molecular characteristics of the isolates were studied by MLST, MLVA and PFGE.Results: Fifty-four S. flexneri isolates possessed four typical biochemical characteristics of Shigella. The prevalences of ipaH, virA, ipaBCD, ial, sen, set1A, and set1B were 100%, 100%, 77.78%, 79.63%, 48.15% and 48.15%, respectively. None of the studied strains possessed the stx gene. Regarding the differences in virulence factor distributions, the 54 S. flexneri isolates fell into seven gene profile types. Among these VTs, VT4 and VT6 were the most common, accounting for 74.07% of all VTs. MLVA based on 8 VNTR loci discriminated the isolates into 39 different MTs, PFGE based on NotI digestion divided the 54 isolates into 31 PTs, and MLST based on 15 housekeeping genes differentiated the isolates into 7 STs, with 1 ST (ST227) being novel.Conclusion: Our findings provide baseline information on the distribution of virulence genes in and the molecular characteristics of S. flexneri collected from diarrheal calves, which is a potential threat to public safety. These data will be important for addressing clinical and epidemiological issues regarding Shigellosis.
Arterial and vein related catheters thrombosis in a patient with COVID-19 : a case report.
Authors: Haoudar Amal , Kantri Aziza , Ziati Jihane , Aidaoui Karim , Elkettani Chafik 
Published: May 2020

Abstract
Patients with acute respiratory distress syndrome due to infection with the novel coronavirus SARS-COV2 are currently considered at high risk of developing thromboembolic complications in both venous and arterial vessels. The use of anticoagulants for preventive or curative purposes should be considered to reduce the risk of thromboembolic events. We report a case of a patient with severe COVID-19 acute respiratory distress syndrome who consecutively developed a right femoral deep vein thrombosis related to the femoral central line and acute ischemia of the left upper limb related to a radial arterial line. He was under a therapeutic dose of low molecular weight heparin twice a day three days before. The femoral vein was free of thrombosis while the central line was placed under a duplex ultrasound.Thromboembolic events can occur in patients with severe COVID-19 despite therapeutic anticoagulants. Close monitoring of vascular access with duplex ultrasound may be required.
Forecasting COVID-19 Pandemic: A Data-Driven Analysis
Authors: Nabi Khondoker Nazmoon 
Published: May 2020

Abstract
In this paper, a new Susceptible-Exposed-Symptomatic Infectious-Asymptomatic Infectious-Quarantined-Hospitalized-Recovered-Dead (SEIDIUQHRD) deterministic compartmental model has been proposed and calibrated for interpreting the transmission dynamics of the novel coronavirus disease (COVID-19). The purpose of this study is to give a tentative prediction of the epidemic peak for Russia, Brazil, India and Bangladesh which could become the next COVID-19 hotspots in no time by using a Trust-region-reflective (TRR) algorithm which one of the well-known real data fitting techniques. Based on the publicly available epidemiological data from late January until 10 May, it has been estimated that the number of daily new symptomatic infectious cases for the above mentioned countries could reach the peak around the beginning of June with the peak size of 15, 774 (95% CI, 13,814-17,734) symptomatic infectious cases in Russia, 26, 449 (95% CI, 23,489-29,409) cases in Brazil, 9, 504 (95% CI, 8,378-10,630) cases in India and 2,209 (95% CI, 1,878-2,540) cases in Bangladesh. As of May 11, 2020, incorporating the infectiousness capability of asymptomatic carriers, our analysis estimates the value of the basic reproduction number (R0) as of May 11, 2020 was found to be 4.234 (95% CI, 3.764-4.7) in Russia, 5.347 (95% CI, 4.737-5.95) in Brazil, 5.218 (95% CI, 4.56-5.81)in India, 4.649 (95% CI, 4.17-5.12) in the United Kingdom and 3.53 (95% CI, 3.12-3.94) in Bangladesh. Moreover, Latin hypercube sampling-partial rank correlation coeffcient (LHS-PRCC) which is a global sensitivity analysis (GSA) method is applied to quantify the uncertainty of our model mechanisms, which elucidates that for Russia, the recovery rate of undetected asymptomatic carriers, the rate of getting home-quarantined or self-quarantined and the transition rate from quarantined class to susceptible class are the most influential parameters, whereas the rate of getting home-quarantined or self-quarantined and the inverse of the COVID-19 incubation period are highly sensitive parameters in Brazil, India, Bangladesh and the United Kingdom which could signicantly affect the transmission dynamics of the novel coronavirus. Our analysis also suggests that relaxing social distancing restrictions too quickly could exacerbate the epidemic outbreak in the above-mentioned countries.
Correlation between Land Surface Temperature and Urban Heat Island with COVID-19 in New Delhi, India
Authors: Mukherjee Semonti , Debnath Aniruddha 
Published: May 2020

Abstract
This study aims to analyses the correlation between Urban Heat Island (UHI), Land Surface Temperature (LST) with COVID-19 (Coronavirus disease) pandemic in New Delhi, India. This study engaged a secondary data analysis of surveillance data of COVID-19 from Ministry of Health and Family Welfare Government of India and temperature data from MODIS (MOD11A1 & MOD11A2). The significant relation in between COVID-19 confirmed cases and LST in day time postulate a positive significance (p value <0.05) with R2=81% and for night time this is R2= 86%.
Antioxidant Activity of Uric Acid and its Correlation with Oxidative Stress and Endothelial Dysfunction in Type 2 Diabetes Mellitus
Authors: Prasad P. Siva , Prasad P. Hari , Sudhakar T. , Rajkumar B. , Raj Adithya , Bheem K. Jai 
Published: May 2020

Abstract
Objective: Uric acid is an end product of purine metabolism and it has two different functions such as pro-oxidant and anti-oxidant. Where, pro-oxidant and anti-oxidants are opposite in action. Oxidative stress and endothelial dysfunction are a foremost cause of complications in diabetes mellitus, where uric acid may play a major role in this process. Hence, the present study has been designed to evaluate antioxidant activity of uric acid and its correlation with oxidative stress and endothelial dysfunction in type 2 diabetic subjects. Methods: We included 120 subjects in this study with age group of 39 -60 years. Among these 60 were type 2 diabetic subjects and 60 were healthy controls. The estimation of biochemical parameters such as blood sugar, lipid profile, uric acid, and homocysteine are measured in fully auto-analyzer with well recognized methods. MDA measured by TBARS method, total antioxidant capacity as FRAP and NO estimated by Kinetic cadmium method in spectrophotometer. Results: The study was found significant elevation of triglyceride, LDL and MDA and significant lower level of FRAP and NO in T2DM than healthy control. Uric acid was insignificant in T2DM compared to healthy control. However, uric acid has significant correlation with FRAP (r=0.2116, p=0.02) and moderate correlation with triglyceride (r=0.1736, p=0.0579) and homocysteine (r=0.1779, p=0.0519). MDA was negatively and NO was positively correlated with uric acid but statistically insignificant. Conclusion: We have found antioxidant activity of uric acid where it was determined by significant positive correlation with FRAP in type 2 diabetes mellitus.
STUDY OF MESH REFINEMENT ON THE AERODYNAMIC COEFFICIENTS FOR NACA2412 PROFILE WITH DIFFERENT ANGLE OF ATTACK AND k - w TURBULENCE MODEL
Authors: Oliveira Nícolas Lima , Loureiro Eric Vargas , Hallak Patrícia Habib 
Published: May 2020

This work presents the studies obtained using OpenFOAM OpenSource Computational Fluid Dynamics (CFD) Software. Experiments were performed to predict lift coefficient and drag coefficient curves for the NACA2412 profile. Subsequently, the results obtained were compared with the results of the bibliography and discussed.
Cyanobacteria – insidious foe of the skin?
Authors: Drobac Backović Damjana , Tokodi Nada , Nikolin Borislava , Svirčev Zorica 
Published: May 2020

AbstractCyanobacteria are ancient photosynthetic microorganisms that shaped today's atmosphere. Anthropocentric and irresponsible activities are changing the atmosphere which favor the frequent occurrence and mass development of cyanobacteria. Extensive cyanobacterial blooming causes numerous problems, including negative effects on human skin. Climate change, depletion of ozone layer, and the increased ultraviolet radiation also affect the skin and lead to more frequent occurrence of skin cancer. This research, for the first time, attempts to establish a connection between these two factors, or whether, in addition to ultraviolet radiation, cyanobacteria can influence the incidence of melanoma. With this objective in mind, an epidemiological investigation was conducted in Vojvodina, Serbia. It was observed that the incidence of melanoma was higher in municipalities where water bodies used for recreation, irrigation and fishing are blooming; however, results could be considered as inconclusive, because of the restrictions in the cancer database. Nevertheless, results gathered from the reviewed literature support the hypothesis that cyanobacteria could be a new potential risk factor for melanoma, while climate change could be a catalyst that converts these potential risk factors into cofactors, which act synergistically with the main risk factor – ultraviolet radiation – and induce an increase of melanoma incidence.
Forecasting water demand under climate change using artificial neural network: a case study of Kathmandu Valley, Nepal
Authors: Shrestha Manish , Manandhar Sujal , Shrestha Sangam 
Published: May 2020

Abstract
With a water demand of 370 MLD, Kathmandu Valley is currently facing a water shortage of 260 MLD. The Melamchi Water Supply Project (MWSP) is an interbasin project aimed at diverting 510 MLD of water in three phases (170 MLD in each phase). Phase I of the project was expected to complete by 2018. Water demand forecasting is the first and important activity in managing water supply. Using the socio-economic factors of number of connections, water tariff and ratio of population to number of university students and climatic factor of annual rainfall, artificial neural network (ANN) was used to predict the water demand of Kathmandu Valley until the year 2040. The analysis suggests that, even after the completion of Phase I of MWSP, the water scarcity in the valley will be 160 MLD in 2020. Therefore, Phase II of MWSP should be completed within 2025 and Phase III should be completed by 2040. The result of this study aids KUKL for better management of the water system. In addition, this research can help in decision making to construct the second and third phase for MWSP, the construction date of which still has not been decided.
An optimal design strategy of decentralized storage tank locations for multi-objective control of initial rainwater quality
Authors: Li Huifeng , Lu Lijun , Huang Xiangfeng , Shangguan Haidong , Wei Zhongqing 
Published: May 2020

Abstract
In recent years, frequent non-point source pollution has raised serious challenges for urban water environmental management. The efficiency and cost of water quality storage tanks, which can prevent and control urban pollution effectively, are significantly affected by their locations. However, few studies have determined the location of decentralized storage tanks with consideration of the characteristics of initial rainwater quality, which has led to unsatisfactory or extravagant design. Therefore, a new design strategy is proposed to optimize the locations of water quality storage tanks using the InfoWorks ICM model in this study. It includes two basic steps. Firstly, the pollution severity of each node in the corresponding subcatchment is evaluated and ranked through the matter element analysis method and analytic hierarchy process. Secondly, all the nodes are precisely sorted by their excessive multiples using the single factor index method. Its application in the design of the decentralized storage tank locations in Fuzhou, China, proved that the proposed strategy can reduce the total volume of decentralized storage tanks to 0.38 times that of a terminal tank. The strategy presented in this study may also be useful in other research on storage tank design in urban pollution prevention and control systems.
Resveratrol Induces Apoptosis and Attenuates Proliferation of MCF-7 Cells in Combination with Radiation and Hyperthermia
Authors: Amini Peyman , Nodooshan Saeedeh Jafari , Ashrafizadeh Milad , Eftekhari Seyed-Mohammad , Aryafar Tayebeh , Khalafi Leila , Musa Ahmed Eleojo , Mahdavi Seyed Rabie , Najafi Masoud , Farhood Bagher 
Published: May 2020

Aim:
In the current in vitro study, we tried to examine the possible role of
resveratrol as a sensitizer in combination with radiotherapy or hyperthermia.

Background:
Breast cancer is the most common malignancy for women and one of the most
common worldwide. It has been suggested that using non-invasive radiotherapy alone
cannot eliminate cancer cells. Hyperthermia, which is an adjuvant modality, induces cancer
cell death mainly through apoptosis and necrosis. However, cancer cells can also develop
resistance to this modality.

Objective:
The objective of this study was to determine possible potentiation of apoptosis
when MCF-7 cells treated with resveratrol before hyperthermia or radiotherapy.

Methods:
MCF-7 cancer cells were treated with different doses of resveratrol to achieve
IC50%. Afterwards, cells treated with the achieved concentration of resveratrol were exposed
to radiation or hyperthermia. Proliferation, apoptosis and the expression of pro-apoptotic
genes were evaluated using flow cytometry, MTT assay and real-time PCR. Results for each
combination therapy were compared to radiotherapy or hyperthermia without resveratrol.

Results:
Both irradiation or hyperthermia could reduce the viability of MCF-7 cells.
Furthermore, the regulation of Bax and caspase genes increased, while Bcl-2 gene
expression reduced. Resveratrol potentiated the effects of radiation and hyperthermia on
MCF-7 cells.

Conclusion:
Results of this study suggest that resveratrol is able to induce the regulation of
pro-apoptotic genes and attenuate the viability of MCF-7 cells. This may indicate the
sensitizing effect of resveratrol in combination with both radiotherapy and hyperthermia.
New Serotoninergic Ligands Containing Indolic and Methyl Indolic Nuclei: Synthesis and In Vitro Pharmacological Evaluation
Authors: Magli Elisa , Severino Beatrice , Corvino Angela , Perissutti Elisa , Frecentese Francesco , Saccone Irene , Giordano Flavia , Castro Marián , Brea José , Loza María I. , Santagada Vincenzo , Caliendo Giuseppe , Fiorino Ferdinando 
Published: May 2020

Background:
Serotonin is an important biogenic amine and is implicated in wideranging
physiological and physiopathological processes. Pharmacological manipulation of the serotoninergic
system is believed to have a great therapeutic potential.

Objectives:
In order to identify selective ligands for 5-HT1A, 5-HT2A and 5-HT2C receptors two series
of 4-substituted piperazine derivatives, bearing indolic or methyl indolic nuclei, were synthesized.

Methods:
All the compounds, synthesized by standard solution methods, were evaluated for 5-
HT1A, 5-HT2A and 5-HT2C receptors. The highest affine and selective compounds have been
evaluated also on dopaminergic (D1 and D2) and adrenergic (α1A and α2A) receptors.

Results:
Several of the newly synthesized molecules showed affinity in the nanomolar range for 5-
HT1A, 5-HT2A and 5-HT2C receptors and moderate to no affinity for other relevant receptors (D1,
D2, α1A and α2A).

Conclusion:
Compounds 7f and 10a showed a nanomolar affinity towards 5-HT1A with an in vitro
pharmacologic profile compatible with antipsychotic drugs.
Exogenous Expression of WNT7A in Leukemia-Derived Cell Lines Induces Resistance to Chemotherapeutic Agents
Authors: Alvarez-Zavala Monserrat , Barreto-Vargas Christian , Torres-Reyes Luis A. , De la Peña-Castro Roberto F. , Aguilar-Lemarroy Adriana , Jave-Suarez Luis F. 
Published: May 2020

Background:
Dysregulations of the WNT pathway are implicated in the malignant transformation of
different types of neoplasia. WNT7A is expressed in normal peripheral lymphocytes, but is decreased in the
tumoral counterpart. Furthermore, the treatment of leukemic cells with recombinant WNT7A decreases proliferation,
suggesting its possible use as a therapeutic biomolecule. This study aimed to evaluate the concomitant
action of WNT7A and different chemotherapeutic agents over proliferation and cell death of leukemia/
lymphoma derived cell lines.

Methods:
Ectopic expression of WNT7A was induced in CEM and BJAB cell lines by using a lentiviral system.
RNA expression was analyzed by microarrays and qPCR, and protein expression was determined by Western
Blot. Cell proliferation was measured by cell counting, metabolic activity by WST-1 assay, cell death and DNA
content by flow cytometry.

Results:
WNT7A ectopic expression was shown to decrease cell proliferation, but the apoptosis rate of leukemic
cells was not altered. Moreover, these cells acquired resistance to doxorubicin, vincristine and MG-132. Cell
cycle analysis reveals a decrease in G1 and an increase in S and G2 phases with a further upregulation of senescence-
associated genes. Microarray analysis reveals that most gene expression changes were related to cancer
and metabolic associated pathways. All those changes appear to be independent of the WNT canonical pathway
regulation.

Conclusion:
WNT7A negatively regulates cell proliferation in leukemic cell lines and promotes resistance to
chemotherapeutic agents by inducing a senescence-like phenotype independently of the WNT canonical pathway.
EFFECT OF ADVERTISED POLYHERBAL (AYURVEDIC) APHRODISIAC FORMULATIONS ON POTENT MALE ALBINO RATS
Authors: G KANASE VANITA , KINI RIDDHI , MANIYAR DRASHTI , SHETTIGAR SHRUTI 
Published: May 2020

Objective: The objective of the study was to study aphrodisiac activity of polyherbals on albino Wistar rats.
Methods: Play Win and Ayurex are examples of the already marketed polyherbal formulations (PHFs), which are used in this study to determine their mating behavior and mating performance test activities in albino Wistar rats.
Results: Both the test drugs Play Win and Ayurex created noteworthy increase in the mounting behavior of animals. It was also observed that mounting behavior activity (number of mounts) in Play Win, in the 1st h and the 3rd h, was greater than Ayurex. Administration of suspension of a single dose of Play Win, Ayurex, and sildenafil citrate concluded in the increase in the mating performance of the rats. The result of Play Win was found to be less than that of Ayurex and nearly the same as that of sildenafil citrate.
Conclusion: From the research of the above-mentioned formulations with comparison to the standard drug (sildenafil), it is concluded that the PHFs are capable of producing aphrodisiac activity.