bluuebunny/crossref_metadata_2025_split huggingface dataset:
Serglycin-Mediated Selective Reactivation of the Novel YAP/CRISPLD2 Developmental Axis Promotes Metastasis and Sorafenib Resistance in Hepatocellular Carcinoma
Authors: HU HAO , Xu Liang , Huang Tie-Jun , Luo Fei-Fei , Wang Jing , Mei Yan , Zheng Li-Sheng , Peng Li-Xia , Meng Dong-Fang , Zhang Zhan-Fei , Zhang Shi-Jun , Qian Chao-Nan , Huang Bi-Jun 
Published: December 2020

Abstract
Background: The extracellular matrix (ECM) component serglycin promotes the epithelial-to-mesenchymal transition (EMT) and metastasis in an autocrine manner. However, the detailed mechanism underlying the roles of serglycin in Hepatocellular carcinoma (HCC) metastasis progression remains to be clarified.Methods: Analyzing 123 patients’ serum by ELISA to investigate the association between serglycin expression and HCC metastasis and prognosis. Serglycin, CRISPLD2, and YAP were overexpressed or knocked down with vector or RNAi. The RNA - sequence was used to screen serglycin downstream effectors, followed by bioinformatics, ChIP-PCR, luciferase, and promoter site mutation to identify novel target genes. The metastatic abilities of serglycin were demonstrated by a series of in vitro and in vivo experiments. Immunofluorescence, flow cytometry, and western blotting were carried out to demonstrate the potential mechanisms of serglycin.Results: We observed that serglycin was overexpressed in HCC tissues and serum, and its level was associated with metastasis and poor prognosis. By gain- and loss-of-function approaches, we found serglycin affects migratory motility and metastatic capacity in vitro and in vivo, and it's significantly associated with the stemness-like properties. Interestingly, serglycin activated the effector YAP of Hippo pathway, and further study verified the crucial role of serglycin/YAP in tumorigenesis by the DEN-induced HCC mouse model. Furthermore, the CRISPLD2 was selectively upregulated by serglycin, the axis of serglycin / YAP / CRISPLD2 regulated metastatic capacity in HCC cells, it revealed CRISPLD2 was the direct target gene of serglycin-mediated YAP/TEAD1 complex. Besides, serglycin-mediated YAP pathway enhanced cell sorafenib resistance, sorafenib combined with verteporfin reversed serglycin-mediated cellular insensitivity to sorafenib therapy.Conclusions: Autocrine ECM serglycin plays a crucial role in the process of stemness maintenance, tumorigenesis, and metastasis via selective reactivation of the novel YAP/CRISPLD2 developmental axis promotes metastasis and sorafenib resistance in Hepatocellular carcinoma, YAP-TEAD1 inhibitor verteporfin reversed serglycin-mediated cellular sorafenib resistance.
MeDIP-Seq and RNA-Seq Releaved TGFB2 Associated with Disease Resistance to ALV-J Infection in Chickens
Authors: Yan Yiming , Zhang Huihua , Gao Shuang , Zhang Huanmin , Zhang Xinheng , Chen Weiguo , Lin Wencheng , Xie Qingmei 
Published: December 2020

Abstract
BackgroundAvian leukosis virus subgroup J (ALV-J) is an oncogenic virus that causes serious economic losses in the poultry industry; unfortunately, there is no effective vaccine. Therefore, ALV-J-susceptible and -resistant chickens had been screened in this study. DNA methylation plays a crucial role in several biological processes, and an increasing number of diseases were proven to be related to alterations of DNA methylation. Thus, we generated and provide the genome wide genome-expression and DNA-methylation profiles by MeDIP-Seq and RNA-Seq in ALV-J-susceptible and -resistant chickens.Results8304 DMRs were identified by MeDIP-Seq analyses and 515 differentially expressed genes were identified by RNA-Seq analysis. Through the results of integration analysis, we screened six candidate genes for screening ALV-J-resistant chickens that were aberrant methylation in the promoter region. Furthermore, functional analyses showed that overexpression of TGFB2 promoted ALV-J replication, which suggested TGFB2 played an important role in disease resistance to ALV-J infection in chickens.ConclusionsThe present study provides new insights into understanding epigenetic changes in ALV-J-susceptible and -resistant chickens, and results provide a basis for breeding disease-resistant-chicken lines.
MK801 Regulates the Expression of Key Osteoarthritis Factors in Osteoarthritis Synovial Fibroblasts Through Complement C5
Authors: Huang Zhi , Feng Yuanyu , Zhu Xiaoxi , Wang Lin , Lu Wei 
Published: December 2020

Abstract
Background: Osteoarthritis is currently one of the most common chronic diseases. As life expectancy increases, its prevalence and incidence are expected to rise. At present, more and more evidences prove the correlation between the complement system and OA. This study aims to investigate complement C5's influence on the effect of MK801 on osteoarthritis synovial fibroblasts (OA-SFs).Methods: We used IL-1b to induce OA-SFs derived from mice to obtain OA-SFs. And we performed RT-PCR and Western Blot assays to evaluate the expression levels of associated mRNA and protein. The alteration of MAC expression on OA-SFs cell membrane was evaluated by immunofluorescence assay. The expression of related inflammatory factors of OA-SFs was evaluated by ELISA experiment.Results: MK801 could significantly inhibit the expression of osteoarthritis (OA) marker factors, such as: membrane attack complex (MAC), tumor necrosis factor-α (TNF-α) and matrix metalloproteinase-13 (MMP13). Meanwhile, MK801 can significantly inhibit the expression of complement C5 (C5) in OA-SFs. Immunofluorescence assay showed that MAC expression on OA-SFs cell membrane was significantly inhibited by MK801. The nucleo-plasmic separation experiment demonstrated that MK801 could significantly inhibit the activation of Nuclear factor-κB (NF-κB) signaling pathway in OA-SFs. Futhermore, koncking down the expression of C5 reversed the inhibition MK801 on the expression of OA-SFs inflammatory factors.Conclusions: These results illustrated two points: first, MK801 inhibited the generation of MAC and the release of inflammation factors in OA-SFs through C5; second: MK801 inhibited the activation of NF-κB signaling pathway in OA-SFs.
Normal Sudanese Spirometeric Values for Police Men
Authors: Bakhit Barakat Mohazab , Musa Omer Abd_elaziz , Malla Mohanad Hassan 
Published: December 2020

Abstract
BackgroundSpirometric values for police officers in the police hospital, Khartoum, Sudan were observed to show higher values than predicted.1 Reference values are important for diagnosis of asthma and chronic bronchitisMethodsFor deriving a new reference value for adult police men, a cross- sectional study was performed on 161 adult male police officers, aged 20–50 years in (June- December 2012) in Khartoum state in different police units. Data were obtained through a questionnaire, pulmonary function testing and taking anthropometric measurements, forced vital capacity (FVC), forced expiratory volume in first second (FEV1) and peak expiratory flow rate (PEFR) were measured using a microplus spirometer and a peak flow meter. Lung function values and anthropometric measurements were correlated and regression equations were derivedResultsAdult police males had a significantly higher FVC, FEV1and PEFR [3.96 ± 0.56 L , 3.61 ± 0.52 L and 564.1 ± 70.4 L/min respectively] than those of normal adult Sudanese males [3.68 ± 0.22 L , 3.35 ± 0.23 L and 505.9 ± 19.1L/min respectively]. A positive correlation was found between lung function and height and a negative correlation with age. Regression equations of normal predicted values were derived for police officer FVC (L) [0.027 X (cm) – 0.039 X A (years) +0.345], FEV1 (L) [0.0264 X (cm) – 0.036 X A (years) +0.0105] and PEFR (L/min) [1.599 X (cm) – 4.207 X A (years) + 329.349.Conclusionsit seems that the continuous training of police officers produced higher lung volumes and a separate reference values tables should be used for them
Comparison of Hospitalized Patients with Severe Pneumonia Caused by COVID-19 and Highly Pathogenic Avian Influenza (H7N9): A Retrospective Study from A Designated Hospital
Authors: Gu Binbin , Yao Lin , Zhu XinYun , Tang Pei-jun , Chen Cheng 
Published: December 2020

Abstract
Background: Considerable attention has been focused on clinical features of Coronavirus Disease 2019 (COVID-19), it is also important for clinicians to differentiate it from influenza virus infections.Methods: The clinical data of 23 cases of H7N9 and 23 cases of COVID-19 with severe pneumonia were collected. The comparisons were performed with the t test, Mann-Whitney U test, Fisher exact test or the chi-squared test, and multivariable logistic regression analysis.Results: All of the cases were under the circumstance of sufficient medical staff and medical supplies. Radiologically, severe COVID-19 patients had less consolidation and pleural effusion, but more crazy-paving pattern than severe H7N9 patients (p<0.05). Clinically, compared to severe H7N9, severe COVID-19 patients were more inclined to surfer to relative better disease severity score, less secondary bacterial infection, a shorter time to beginning absorption on CT, but a longer duration of viral shedding from the admission (p<0.05). Although more severe H7N9 patients needed non-invasive respiratory support, these two groups ultimately yielded comparable mortality. Based on multiple logistic regression analysis, severe COVID-19 infection was associated with a lower risk of the presence of severe ARDS (OR 0.964, 95% [CI] 0.931-0.998, p=0.040), but exhibited longer duration of viral shedding (OR 0.734, 95% [CI] 0.550-0.980, p=0.036) than severe H7N9 infection.Conclusion: Although the conditions of severe H7N9 patients seemed to be more critical than those of severe COVID-19 patients, the relatively lower mortality of these two severe cases is to be expected in context of sufficient medical supplies.
Prenatal Whole Exome Sequencing Reveals a Novel CDH1 Mutation Associated With Blepharo-Cheilo-Dontic Syndrome
Authors: Peng Ying , Shu Li , Xi Hui , Luo Yingchun , Wang Xiaofeng , Pang Jialun , Yang Shuting , Liang Changbiao , Tang Chengyuan , Wang Hua 
Published: December 2020

Abstract
Background: The cleft lip with or without palate (CL/P) is the most prevalent congenital craniofacial abnormality. This study aims to provide molecular diagnosis for patients with CL/P in a Chinese family, and then offer suggestions for future pregnancy for this family.Methods: Karyotyping, single nucleotide polymorphism array analysis, whole-exome sequencing (WES), and Sanger sequencing were applied to identify the underlying genetic cause for the clinical phenotypes. The functional effect of the identified mutation was evaluated through immunofluorescent analysis of the expression of wild or mutant protein that was transiently expressed. Non-invasive prenatal testing (NIPT) and ultrasound testing were conducted for a new pregnancy of this family. Results: A novel missense mutation (c.1418T>A/p.(Val473Asp)) in CDH1 was identified in the family members affected with CL/P. Functional analysis showed that this mutation changed the subcellular localization of the protein. The NIPT and ultrasound testing respectively revealed the new pregnancy carried no CDH1 mutation and facial dysmorphic features, and a healthy baby was delivered.Conclusion: The affected family members were diagnosed with a syndromic CL/P called Blepharo-Cheilo-Dontic(BCD) Syndrome. This study identified a novel CDH1 mutation for CL/P, expanded the mutation spectrum, and contributed to the genetic diagnosis and counseling of this disorder. This study also provided an example of the application of NIPT for BCD Syndrome.
Psychometric Properties of Postpartum Quality of Life Questionnaires: a Systematic Review and Meta-analysis Protocol
Authors: Mokhtarian-Gilani Tahereh , kariman Nourossadat , Sharif-Nia Hamid , Ahmadi-Doulabi Mahbobeh , Nasiri Malihe 
Published: December 2020

Abstract
Background:The postpartum quality of life refers to women's understanding of their standing in the postpartum crisis that differs depending on their health status, social support, cultural status and values, attitudes, goals and standards. The present systematic review will identify, describe, and critically assess the psychometric properties of postpartum quality of life questionnaires.Methods/Design:A systematic review will be conducted in databases including PubMed, Embase, Scopus, Web of Science, PsycINFO, and CINAHL from January 2000 to January 2020. The psychometric properties (validity and reliability) of the instruments used in the primary studies will be assessed, and the selection, methodological quality assessment and data extraction processes of the studies will be independently assessed by two reviewers with expertise in conducting systematic reviews, so as to minimize potential personal bias. Eligible resources are selected after any lack of consensus is put to debate.The risk of bias is assessed using the COSMIN RISK of Bias checklist, and to evaluate the quality of the studies, the protocol is written based on the PRISMA-P1 standards. The results of the studies will be judged based on good measurement properties, and the results of all the studies are qualitatively summarized to produce a reference for the general quality of the results. The general quality of the evidence will be determined using a modified GRADE method.Discussion:This study assessed the psychometric properties of questionnaires used for assessing postpartum quality of life and its results can be used to identify the most appropriate tool for health applications in measuring postpartum quality of life. Systematic review registration: reference number in PROSPRO CRD42020166301
Effects of Different Integrase Inhibitors on Body Weight in Patients with HIV/AIDS: A Network Meta-Analysis
Authors: Bai Ruojing , Lv Shiyun , Wu Hao , Dai Lili 
Published: December 2020

Abstract
Background: Global antiretroviral therapy has entered the era of integrase strand transfer inhibitor (INSTI). Because INSTIs have the advantages of high antiviral efficacy, rapid virus inhibition, and good tolerance, they have become the first choice in international acquired immunodeficiency syndrome (AIDS) treatment guidelines. However, they may also increase the risk of obesity. There are differences in the effects of different INSTIs on weight gain in Human immunodeficiency virus (HIV) infection / AIDS patients, but there is no evidence-based medical evidence. This study aimed to assess the effect of different INSTIs on body weight in HIV/AIDS patients.Methods: PubMed, Embase, Cochrane Library, China National Knowledge Infrastructure (CNKI), Chinese Biomedical Literature Database (CBM), China Science and Technology Journal Database, and Wanfang databases were searched by computer to screen the relevant literature on INSTI treatment of HIV/AIDS patients, extract the data on weight changes in the literature, and perform network meta-analysis using Stata16.0 software.Results: Eight articles reported weight changes in HIV/AIDS patients, and weight gain was higher after treatment with dolutegravir (DTG) than with elvitegravir (EVG) in HIV/AIDS patients, and the difference was statistically significant [MD = 1.13, (0.18, 2.07)]. The network meta-analysis's consistency test results showed no overall and local inconsistency, and there was no significant difference in the results of the direct and indirect comparison (P > 0.05). The rank order of probability was DTG (79.2%) > Bictegravir (BIC) (77.9%) > Raltegravir (RAL) (33.2%) > EVG (9.7%), suggesting that DTG may be the INSTI drug that causes the most significant weight gain in HIV/AIDS patients.Conclusion: According to the literature data analysis, among the existing INSTIs, DTG may be the drug that causes the highest weight gain in HIV/AIDS patients, followed by BIC.
Clinical Characteristics of Mechanically Ventilated Patients Treated in Non-ICU Settings in Japan: A Retrospective Analysis
Authors: Iwashita Yoshiaki , Morimoto Shinnosuke , Koyabu Sukenari , Maruyama Kazuo , Imai Hiroshi 
Published: December 2020

Abstract
Background:The number of patients requiring mechanical ventilation (MV) is increasing worldwide. Patients requiring MV are commonly managed in an intensive care unit (ICU); however, Japan is unique in that many of these patients are treated in non-ICU settings. The characteristics of these patients, nevertheless, are unknown. We sought to identify disease severity and MV settings of patients in non-ICU settings in Japan.Methods: We retrospectively analyzed the clinical data of Kinan Hospital and Owase General Hospital, where there are no ICUs or ICU physicians. Data for adult patients who required MV for more than 3 d from January through December 2018 in those hospitals were collected.Results:A total of 171 patients received MV; 29 patients were treated for more than 3 d. Of that subset, the mortality rate was 44.8% (13 patients). Thirteen patients survived to discharge, and three were transferred to a higher-level facility. The median age of patients treated for more than 3 d was 80 (72–84) years. The mean Acute Physiologic Assessment and Chronic Health Evaluation II score was 20.9 ± 8.1, and predicted mortality was 0.42 ± 0.25. Tidal volume per predicted body weight was 8.8 ± 2.1 mL/kg; set inspiratory time was 1.6 ± 0.3 sec. Preventable non-ICU death (patients who might have been saved if treated in an ICU setting), was 10.3%.Conclusions:The overall mortality of patients treated with MV in a non-ICU setting in the East Kishu area was not inferior to the mortality if they had been treated in an ICU; however, MV settings should be improved.
Non- Specific Low Back Pain among Nurses in Qassim, Saudi Arabia: A Cross-Sectional Study
Authors: Elmannan Abeer Abuzeid Atta , AlHindi Hajar A. , AlBaltan Reema I. , AlSaif Mariah S. , AlMazyad Nouf S. , AlZurayer Ruba K. , AlRumayh Shouq S. 
Published: December 2020

Abstract
Background: Non-specific Low back pain (LBP) is a complex and multifactorial health problem. Evidence has shown that LBP is an important occupational hazard and nurses are particularly at high risk. While several studies have addressed the prevalence of LBP worldwide, the prevalence of LBP in Saudi Arabia remains unclear. In this study we aimed to estimate the prevalence and associated factors of LBP among nurses in Qassim region, Saudi Arabia. Methods: This was a multicenter cross-sectional study carried out in four major public hospitals in Qassim region. A total of 323 nurses were recruited through a two-stage sampling method. A previously validated questionnaire was used to gather data. The main outcome measures were; LBP prevalence during working life, demographic factors, life-style factors, work-related factors and psychological factors. Multivariable logistic regression analysis was used to determine factors independently associated with LBP. Results: The study showed that LBP prevalence was 65.6%. Over one third of study participants sought treatment for LBP (38.7%). Age and the type of ward were found significantly associated with LBP (aOR: 0.39; 95% CI: 0.19, 0.77; p value=0.007) & (aOR: 0.36; 95%CI: 0.15, 0.86; p value =0.02), respectively. However, gender, working hours, number of patients, stress and smoking were not identified as LBP risk factors in this study.Conclusion: The findings of this study suggest that LBP is a highly prevalent occupational health problem among nurses in Qassim. The highest LBP prevalence in this study was found among nurses aged 20-30 year. Nurses working in the general surgery wards showed a lower risk for LBP. On-the-job training is essential particularly for new and young nurses on proper body mechanics when mobilizing patients or lifting heavy equipment. In addition, there is a need for evidence –based interventions to improve the work-place environment for nurses in hospitals in order to lower LBP prevalence.
KETERLIBATAN AYAH DALAM PENGASUHAN ANAK DENGAN SEPARATION ANXIETY DISORDER
Authors: Siron Yubaedi 
Published: December 2020

Father involvement in parenting is an important factor in the process of child development. However, the involvement of fathers in childcare is very low. The objective of this study is to explore and find out how fathers are involved in parenting of children with separation anxiety disorder. This research uses a qualitative approach. Collecting data in this study using structured interviews. Participants were selected using purposive sampling. Participants in this study were 3 fathers who have children with Separation Anxiety Disorder (ASD). The results showed that the involvement of fathers in childcare plays an important role in the development of children with ASD. Fathers have done a good role in parenting for ASD children, as well as a good relationship between father and children with ASD. Fathers have varied activities to stimulate their ASD children, as well as positive communication by fathers to their children. Fathers give good understanding to their children and provide follow-up on their parenting involvement.
Assessing the impact of PET estimation methods on hydrologic model performance
Authors: Jayathilake Dilhani Ishanka , Smith Tyler 
Published: December 2020

Abstract
Evapotranspiration is a necessary input and one of the most uncertain hydrologic variables for quantifying the water balance. Key to accurately predicting hydrologic processes, particularly under data scarcity, is the development of an understanding of the regional variation of the impact of potential evapotranspiration (PET) data inputs on model performance and parametrization. This study explores this impact using four different potential evapotranspiration products (of varying quality). For each data product, a lumped conceptual rainfall–runoff model (GR4J) is tested on a sample of 57 catchments included in the MOPEX data set. Monte Carlo sampling is performed, and the resulting parameter sets are analyzed to understand how the model responds to differences in the forcings. Test catchments are classified as energy- or water-limited using the Budyko framework and by eco-region, and the results are further analyzed. While model performance (and parameterization) in water-limited sites was found to be largely unaffected by the differences in the evapotranspiration inputs, in energy-limited sites model performance was impacted as model parameterizations were clearly sensitive to evapotranspiration inputs. The quality/reliability of PET data required to avoid negatively impacting rainfall–runoff model performance was found to vary primarily based on the water and energy availability of catchments.
A systematic review of microorganisms as indicators of recreational water quality in natural and drinking water systems
Authors: Lugo José Luis , Lugo Elkyn Rafael , Puente Mario de la 
Published: December 2020

Abstract
The purpose of this review was to highlight the most frequent biological indicators used to estimate the microbiological quality of drinking and recreational water. It was observed that the incorporation of other microbiological indicators should be considered to strengthen the decision-making process on water quality management and guarantee its safe consumption in recreational activities.