bluuebunny/crossref_metadata_2025_split huggingface dataset:
Performance Liquid Chromatography/Tandem Mass Spectrometry
Authors: Chen Wen-Ling , Hsu Tzu-Fang , Chen Chia-Yang 
Published: February 2020

Abstract
A sensitive method was developed using ultra-high-performance liquid chromatography (UHPLC)/MS/MS with positive electrospray ionization for determining aflatoxin M1 (AFM1) in milk and milk powder. A 50 mL quantity of low-fat liquid milk containing 100 ng/L AFM1 was prepared using immunoaffinity columns with a mean recovery rate of 79% (n = 3). UHPLC columns (BEH C18, BEH HILIC, and HSS T3) greatly reduced the chromatographic time and lowered the instrumental detection limits (IDLs) 16 to 58 times compared to an HPLC column (Betabasic C18). The HSS T3 column was chosen because it provided a low IDL (0.11 pg) and the lowest ion suppression of signal intensity (63.4%) among the tested columns. Matrix-fortified calibration curves were used for quantification and showed good linearity (r &gt; 0.997) at 0.05–500 ng/mL. The LOD was 0.18 ng/kg for milk and 2.08 ng/kg for milk powder, based on the signal intensity of the confirmatory product ion (m/z 259.1), which was less abundant than the quantitative product ion (m/z 273.1). Certified reference materials of milk powder
Validation Study of a Rapid ELISA for Detection of Histamine in Tuna
Authors: Lupo Anthony , Mozola Mark 
Published: February 2020

Abstract
Neogen Corp. has developed an improved Veratox histamine test kit for the detection of histamine in tuna tissue. The purpose of this study was to validate the method under the requirements of AOAC Research Institute (RI) Performance Tested MethodsSM. Three AOAC Official MethodsSM for histamine (954.04, 957.07, and 977.13) and one ELISA method have been performance tested by the AOAC RI. The most popular is AOAC Official MethodSM977.13, the fluorometric method, which is considered the reference method, but is complicated and time-consuming. The need for a rapid ELISA test kit to be validated by the AOAC RI exists.
Development of Extraction Methods for Speciation Analysis of Selenium in Aqueous Extracts of Medicinal Plants
Authors: Kolachi Nida F , Kazi Tasneem G , Afridi Hassan I , Khan Sumaira , Baig Jameel A , Wadhwa Sham K , Shah Abdul Q , Shah Faheem 
Published: February 2020

Abstract
Advanced extraction methods have been developed for direct speciation of dissolved inorganic and organic selenium (Se) species in aqueous extracts of medicinal plants (MPs). The inorganic species of Se (SeIV and SeVI) were separated from organic forms by adsorbing inorganic Se on alumina, while the organic Se was not retained. The retained inorganic Se species was eluted with 10 mL 0.2 M HCl. The total inorganic Se species was determined after prereduction of SeVI into SeIV with concentrated HCl. The SeIV in the eluent and total inorganic Se species were then complexed with diethyldithiocarbamate. The resultant complexes were entrapped in the nonionic surfactant Triton X-114. The total Se, organic Se, total inorganic Se, and SeIV species were determined by electrothermal atomic absorption spectrometry with a modifer. The SeVI concentration was obtained by subtracting SeIV from total inorganic Se contents. The main factors affecting the methodologies were investigated in detail. Under the optimized experimental conditions, the LOD for SeIV was 50 µg/L. Among dissolved inorganic and organic Se species in aqueous extracts of MPs, organic Se species were present in the range of 74–84%, SeIV 3.62–7.47%, and SeVI 12.4–18.57% of total Se contents.
Specific PCR Detection of Tiger, Leopard, and Lion Ingredients from Test Samples
Authors: Cao Jijuan , Xu Junyi , Liu Ran , Yu Ke , Wang Changwen 
Published: February 2020

Abstract
A PCR method was developed for specifc detection of tiger, leopard, and lion DNA from test specimens for inspection and quarantine or for law-enforced animal protection. Three pairs of specifc primers were designed based on the mitochondrial cytochrome b gene of tiger, leopard, and lion and used in the PCR testing. To mimic the effect of food processing on the sensitivity of the test, the tiger muscle and bovine bonemeal powder samples were treated at 133°C for 30 min. At this processing condition, the method was sensitive enough to detect as low as 0.05% of tiger-derived ingredients from the mixed bonemeal powders. The data demonstrate that our PCR method is convenient and economic, with high sensitivity and repeatability, and can be used to detect and identify tiger, leopard, and lion ingredients from various test samples.
Eleutherosides in Aerial Parts of Eleutherococcus Species Cultivated in Poland
Authors: Załuski Daniel , Smolarz Helena Danuta , Szpilewska Monika 
Published: February 2020

Abstract
Many Eleutherococcus species grow in Siberia, China, Korea, Japan, and the Philippines. The most well known is Eleutherococcus senticosus, which contains pharmacologically active compounds, such as eleutherosides, flavonoids, vitamins, and complex polysaccharides. E. senticosus owes its medicinal properties mainly to eleutherosides. The objective of this study was to determine eleutherosides B, E, and E1 in the aerial parts of different Eleutherococcus species. The eleutherosides were extracted with ethanol, and the extracts were cleaned by SPE on C18 columns, and then analyzed by HPTLC. Silica gel plates with fluorescence indicator, designated F254, were used with chloroform–methanol–water (70 + 30 + 4, v/v/v) and chloroform–methanol–toluene–ammonium hydroxide (9 + 6 + 3 + 2, v/vv/v) mobile phases. Two-step elution with these mobile phases was used for the development of chromatograms. Eleutherosides were visualized by derivatization with Liebermann-Burchard reagent. This reagent was used for the first time to detect eleutherosides. Eleutherosides B, E, and E1 were detected in the fruits of the investigated species. E. senticosus contained three of the investigated compounds, and E. sessiliflorus, E. gracilistylus, and E. divaricatus two compounds each.
Method for the Rapid Determination of Protein in Meats Using the CEM Sprint™ Protein Analyzer: Collaborative Study
Authors: Moser Cindy , Herman Kathy ,   Collaborators:, Barnhardt K , Ceizyk M , Chriscoe T , Conch M , Haneklaus A , Herman K , Hetzler M , Holton P , Hughes V , Kalinska H , Martin J , Steiner G , Temples M , Trumble D , Vargus A , Weber M , Weidemann J , Zielke G 
Published: February 2020

Abstract
A collaborative study was conducted to determine the protein content of raw and processed meat products by a protein-tagging and colorimetric technique. Meat products were prepared following AOAC Official MethodSM 983.18 and analyzed using CEM Corporation's Sprint Rapid Protein Analyzer. Sprint provides protein results by combining an accurately weighed test portion with a known amount of dye-binding agent. The dye-binding agent binds with the lysine, histidine, and arginine, as well as the n-terminus of the proteins commonly found in raw meat and processed meat products. Results are displayed and reported by the Sprint as a percentage (g/100 g) of protein. Ten blind duplicate study samples were sent to 10 collaborating laboratories in the United States. The within-laboratory (repeatability) relative standard deviation (RSDr) ranged from 0.91 to 3.04%, and between-laboratories (reproducibility) relative standard deviation (RSDR) ranged from 1.50 to 3.41% for protein. The method is recommended for Official First Action.
Determination of Rufinamide in the Presence of 1-[(2,6-Difluorophenyl)Methyl]-1H-1,2,3-Triazole-4 Carboxylic Acid Using RP-HPLC and Derivative Ratio Methods as Stability Indicating Assays to Be Applied on Dosage Form
Authors: Hassib Sonia T , Hashem Hanaa M A , Mahrouse Marianne A , Mostafa Eman A 
Published: February 2020

Abstract

Background
Rufinamide is a triazole derivative that is structurally dissimilar to other marketed antiepileptic drugs, has been assumed a marketing authorization, by the European Union and FDA, for use as a complementary therapy for seizures associated with Lennox-Gastaut syndrome.

Objective
This work is concerned with development of two methods for determination of rufinamide (RUF) in presence of 1-[(2,6-difluorophenyl)methyl]-1H-1,2,3-triazole-4 carboxylic acid as its alkaline degradation product in dosage form.

Methods
The first method was capable of determing RUF in the presence of its alkaline degradation product and in dosage form. Kromasil C8 column and mobile phase consisting of acetonitrile–water (50:50, v/v) were used and UV detection at 210 nm. In the second method, first derivative ratio spectrophotometry, RUF was determined by measuring peak amplitude at 269.5 nm over 5–30 μg/mL.

Results
The linearity range of RUF was 10–90 μg/mL for HPLC method covering its therapeutic range with r2 = 0.9999. Forced degradation under alkaline conditions was carried out, the degradation product was isolated and its structure was confirmed. Both methods were validated in accordance to ICH guidelines. Statistical analysis revealed no significant difference between obtained results and reported ones.

Conclusion
The present study is useful for therapeutic drug monitoring and routine analysis of RUF in quality control laboratories.

Highlights
Kinetics of the alkaline degradation of RUF was studied by following the concentration of the remaining drug until complete degradation was achieved.
Pictures Do Influence the Decision to Transfer: Outcomes of a Telemedicine Program Serving an Eight-State Rural Population
Authors: Garber Rebecca N , Garcia Edwin , Goodwin Cleon W , Deeter Lyndsay A 
Published: February 2020

Abstract
Triaging burn patients is a daunting task because burn injuries are rare; this inexperience leads to uncertainty in treatment and referral algorithms. Our regional burn center’s catchment area includes eight states. Outlying facilities consult via telephone through the medical center’s transfer center. Referring provider assessments of depth or size of injury infrequently correlates with burn provider’s assessments. This causes over- and under-triage of patients managed outside of burn centers. A quality improvement telemedicine project was developed to allow burn providers to review photos with referring providers to determine best management, provide pertinent education, and initiate appropriate and timely resuscitation. Details tracked include date of service, consulting provider, follow-up education offered, and whether the image reviewed changed or confirmed the requested plan of care. Of the 155 cases between January 2017 and August 2018, 24.5% of patient images changed the initial transfer decision, and 75.5% confirmed the initial plan of care. Of the cases requiring change of plan, 60.5% were down-triaged to outpatient care and 39.5% were up-triaged to transfer. Implementation of this telemedicine program has increased efficiency of resource utilization, timely resuscitation, appropriate transfer of patients requiring admission, and real-time education. Findings suggest advanced practice providers’ assessments are similar to those of referring physicians. These observations may have significant implications on Emergency Medical Treatment and Labor Act (EMTALA) guidelines defining physician to physician consultation and support efficient use of available resources. Telemedicine facilitates access to specialized care and improves fiscal responsibility.
Evaluation of the face advance rate on ground control in the open face area associated with mining operations in Western China
Authors: Song Gaofeng , Wang Zhenwei , Ding Kuo 
Published: February 2020

Abstract
The modern longwall faces in coal bases in Western China are being mined at an increasingly faster rate, yet the consequences of the face advance rate on the ground control and strata movement require further investigation. In this study, two improved physical models with different advance rates are developed to evaluate the roof failure characteristics; the strata movement; the displacement of the strong, massive roof; and the roof movement velocity. The results show that: (i) regular falls of the immediate roof and major falls of the hard roof are observed with the progressive development of the longwall face. Massive fractures on the roof strata extending from the face to the ground surface develop on a major roof fall. (ii) Model I, which has a slower face advance rate, shows a major roof fall interval of 65 m, which is slightly less than the 70 m found by Model II, which advances at a faster rate. Larger strata fractures are observed in Model I, while the gob area of Model II is better filled with waste rock materials. (iii) The displacement and velocity of the hard roof are unnoticeable until a massive roof fall. Maximum displacement occurs on a major roof fall, which is 50 mm for Model I and 30 mm for Model II. The maximum roof movement velocity on a major roof fall is 4.6 cm per min and 5.9 cm per min for Models I and II, respectively.
Clinical experience of pelvic radiotherapy or chemoradiotherapy for postoperative uterine cervical cancer using intensity-modulated radiation therapy
Authors: Yamamoto Takaya , Umezawa Rei , Tokunaga Hideki , Kubozono Masaki , Kozumi Maiko , Takahashi Noriyoshi , Matsushita Haruo , Kadoya Noriyuki , Ito Kengo , Sato Kiyokazu , Tsuji Keita , Shimada Muneaki , Jingu Keiichi 
Published: February 2020

Abstract
The purpose of this study was to reveal treatment outcomes and toxicity after pelvic intensity-modulated radiotherapy (IMRT) for postoperative uterine cervical cancer of Japanese patients. Consecutive patients who were treated with pelvic IMRT for postoperative cervical cancer in our institute were retrospectively analyzed. Relapse-free survival (RFS) and overall survival (OS) were calculated using the Kaplan–Meier estimator, and log-rank tests were used to compare differences. From the database, 62 patients were identified. The pathology was squamous cell carcinoma in 44 patients and other pathology in 18 patients. Of the 62 patients, 35 had high-risk prognostic factors and 27 patients had intermediate-risk prognostic factors. The prescribed radiation doses were 50 Gy in 25 fractions for 58 patients and 50.4 Gy in 28 fractions for 4 patients. One patient received a vaginal cuff boost. Chemotherapy was administered in 36 patients. During the median follow-up period of 50.9 months, there was no locoregional failure. Six patients in the high-risk group relapsed, but none of the patients in the intermediate-risk group relapsed (P = 0.02). The 3-year OS and RFS rates were 98.2% and 90.9%, respectively. Significant factors related to RFS were squamous cell carcinoma pathology (P = 0.02), pathological T stage (P = 0.04), surgical margin status (P &lt; 0.01) and multiple lymph nodes metastases (P &lt; 0.01). Grade 3 or more toxicity occurred in 6 patients. Four patients had obstruction of the intestine, and 2 patients had stenosis of the urinary tract. In clinical practice, the use of pelvic IMRT for postoperative cervical cancer of Japanese patients showed a low rate of toxicity without decreasing the efficacy.
Pioneers or Mere Labor Force? Post–World War II Italian Rural Migration to Brazil and the Legacies of Colonialism
Authors: Gaggio Dario 
Published: February 2020

Abstract
In the early 1950s, the Italian government experimented with organized rural settlement in Brazil and the rest of Latin America. Part of a general effort to ease Italy’s “overpopulation,” rural settlement abroad had special meanings for both Italian and Brazilian elites. These experiments resonated with the memories of mass emigration at the turn of the twentieth century and with the legacy of colonial settlement in Libya and East Africa. These projects also fulfilled ongoing racial and eugenic aspirations in Brazil. Italian rural settlement in Brazil took the form of both employment in the coffee fazendas (estates) and the foundation of relatively homogenous “colonies.” However unsuccessful on their own terms, these experiments provide evidence of the links between colonial knowledge and development practices, and they challenge the widespread assumption that Italians simply repressed their colonial past after the end of fascism and military defeat.
Rapid particle acceleration due to recollimation shocks and turbulent magnetic fields in injected jets with helical magnetic fields
Authors: Nishikawa Kenichi , Mizuno Yosuke , Gómez Jose L , Duţan Ioana , Niemiec Jacek , Kobzar Oleh , MacDonald Nicholas , Meli Athina , Pohl Martin , Hirotani Kouichi 
Published: February 2020

ABSTRACT
One of the key questions in the study of relativistic jets is how magnetic reconnection occurs and whether it can effectively accelerate electrons in the jet. We performed 3D particle-in-cell (PIC) simulations of a relativistic electron–proton jet of relatively large radius that carries a helical magnetic field. We focused our investigation on the interaction between the jet and the ambient plasma and explore how the helical magnetic field affects the excitation of kinetic instabilities such as the Weibel instability (WI), the kinetic Kelvin–Helmholtz instability (kKHI), and the mushroom instability (MI). In our simulations these kinetic instabilities are indeed excited, and particles are accelerated. At the linear stage we observe recollimation shocks near the centre of the jet. As the electron–proton jet evolves into the deep non-linear stage, the helical magnetic field becomes untangled due to reconnection-like phenomena, and electrons are repeatedly accelerated as they encounter magnetic-reconnection events in the turbulent magnetic field.
LY6K promotes glioblastoma tumorigenicity via CAV-1–mediated ERK1/2 signaling enhancement
Authors: Sastry Namratha G , Wan Xuechao , Huang Tianzhi , Alvarez Angel A , Pangeni Rajendra P , Song Xiao , James Charles David , Horbinski Craig M , Brennan Cameron W , Nakano Ichiro , Hu Bo , Cheng Shi-Yuan 
Published: February 2020

Abstract

Background
Lymphocyte antigen 6 complex, locus K (LY6K) is a putative oncogene in various cancers. Elevated expression of LY6K is correlated with poor patient prognosis in glioblastoma (GBM). The aim of this study is to advance our understanding of the mechanism by which LY6K contributes to GBM tumor biology.

Methods
Bioinformatic data mining was used to investigate LY6K expression in relation to GBM clinical outcome. To understand the role of LY6K in GBM, we utilized patient-derived glioma stemlike cells (GSCs) and U87 cells and employed immunoblotting, immunofluorescent staining, radiation treatment, and orthotopic GBM xenograft models.

Results
Our results show that increased expression of LY6K inversely correlates with GBM patient survival. LY6K promotes tumorigenicity in GBM cells both in vitro and in vivo. The mechanism underlying this tumorigenic behavior is enhancement of extracellular signal-regulated kinase 1 and 2 (ERK1/2) signaling. Interestingly, we observed that tumor-promoting LY6K-ERK1/2 signaling is mediated by the interaction of LY6K with caveolin-1, rather than through oncogenic receptor tyrosine kinase–mediated signaling. Moreover, association of LY6K with the cell membrane is crucial for its tumorigenic functions. Finally, DNA methylation maintains LY6K silencing, and hypomethylation of the LY6K promoter increases its expression. In GSCs, ionizing radiation leads to demethylation of the LY6K promoter, thereby increasing LY6K expression and GSC resistance to radiation.

Conclusions
Our study highlights the importance of the contribution of LY6K to GBM tumor biology and suggests LY6K as a potential membrane target for treating GBM.
Laser Interstitial Thermal Therapy for Metastatic Melanoma After Failed Radiation Therapy: A Case Series
Authors: Palejwala Ali H , O’Connor Kyle P , Milton Camille K , Pelargos Panayiotis E , Glenn Chad A , Bohnstedt Bradley N , Algan Ozer , Sughrue Michael E 
Published: February 2020

Abstract

BACKGROUND
Laser interstitial thermal therapy (LITT) is a growing technology to treat a variety of brain lesions. It offers an alternative to treatment options, such as open craniotomy and stereotactic radiosurgery.

OBJECTIVE
To analyze our experience using LITT for metastatic melanoma.

METHODS
This is a retrospective chart review of the patients from our institution. Our case series involves 5 patients who had previously failed radiation treatment.

RESULTS
Our patients have low complication rates and short hospital stays. Both are considerably lower when compared to the literature for metastatic melanoma.

CONCLUSION
LITT is a safe therapy, with few complications and short hospital stays.
HRCT imaging features in representative imported cases of 2019 novel coronavirus pneumonia
Authors: Diao Kaiyue , Han Peilun , Pang Tong , Li Yuan , Yang Zhigang 
Published: February 2020

Abstract
With the spread of novel coronavirus (2019-nCoV) pneumonia, chest high-resolution computed tomography (HRCT) has been one of the key diagnostic tools. To achieve early and accurate diagnostics, determining the radiological characteristics of the disease is of great importance. In this small scale research we retrospectively reviewed and selected six cases confirmed with 2019-nCoV infection in West China Hospital and investigated their initial and follow-up HRCT features, along with the clinical characteristics. The 2019-nCoV pneumonia basically showed a multifocal or unifocal involvement of ground-glass opacity (GGO), sometimes with consolidation and fibrosis. No pleural effusion or lymphadenopathy was identified in our presented cases. The follow-up CT generally demonstrated mild to moderate progression of the lesion, with only one case showing remission by the reducing extent and density of the airspace opacification.
Exploration of Plastid Phylogenomic Conflict Yields New Insights into the Deep Relationships of Leguminosae
Authors: Zhang Rong , Wang Yin-Huan , Jin Jian-Jun , Stull Gregory W , Bruneau Anne , Cardoso Domingos , De Queiroz Luciano Paganucci , Moore Michael J , Zhang Shu-Dong , Chen Si-Yun , Wang Jian , Li De-Zhu , Yi Ting-Shuang 
Published: February 2020

Abstract
Phylogenomic analyses have helped resolve many recalcitrant relationships in the angiosperm tree of life, yet phylogenetic resolution of the backbone of the Leguminosae, one of the largest and most economically and ecologically important families, remains poor due to generally limited molecular data and incomplete taxon sampling of previous studies. Here, we resolve many of the Leguminosae’s thorniest nodes through comprehensive analysis of plastome-scale data using multiple modified coding and noncoding data sets of 187 species representing almost all major clades of the family. Additionally, we thoroughly characterize conflicting phylogenomic signal across the plastome in light of the family’s complex history of plastome evolution. Most analyses produced largely congruent topologies with strong statistical support and provided strong support for resolution of some long-controversial deep relationships among the early diverging lineages of the subfamilies Caesalpinioideae and Papilionoideae. The robust phylogenetic backbone reconstructed in this study establishes a framework for future studies on legume classification, evolution, and diversification. However, conflicting phylogenetic signal was detected and quantified at several key nodes that prevent the confident resolution of these nodes using plastome data alone. [Leguminosae; maximum likelihood; phylogenetic conflict; plastome; recalcitrant relationships; stochasticity; systematic error.]