bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sequencing of 185 Streptococcus thermophilus and identification of fermentation biomarkers
Authors: Liu Wenjun , Wu Linjie , Zhao Jie , Li Weicheng , Wang Yu , Zheng Huijuan , Sun Tiansong , Zhang Heping , Xi Ruibin , sun Zhihong 
Published: September 2020

Abstract
Streptococcus (S.) thermophilus is an important dairy starter in the production of fermented dairy products has important significance, from natural fermentation in the past to industrial production today. While the genetic architecture underlying S. thermophilus traits and phenotypes is largely unknown. Here, we sequenced 185 S. thermophilus strains, which isolated from natural fermented dairy products of China and Mongolia and using comparative genomic and genome wide association study to provide novel point for genetic architecture underlying its traits and phenotypes. Genome analysis of S. thermophilus showed association of phylogeny with environmental and phenotypic features and revealed clades with high acid production potential or with substantial genome decay. A few S. thermophilus isolated from areas with high chloramphenicol emissions had a chloramphenicol-resistant gene CatB8. Most importantly, we defined a growth score and identified a missense mutation G1118698T located at the gene AcnA that were both predictive of acidification capability of S. thermophilus. Our findings provide novel insight in S. thermophilus genetic traits, antibiotic resistant and predictive of acidification capability which both may had huge help in culture starter screening.
Heme relieves the inhibition of proliferation, migration and angiogenesis of HMEC-1 under hyperoxia by inhibiting BACH1
Authors: Jian Lan , Mei Yang , Rongdi Yuan 
Published: September 2020

Abstract
Background
The relatively hyperoxia inhibited vascular endothelial growth factor (VEGF) in retina is the main cause of angiogenesis retardation in phase I retinopathy of prematurity (ROP). Human retinal angiogenesis is related to the proliferation, migration and angiogenesis of microvascular endothelial cells. Previous studies have confirmed that BTB and CNC homology l (BACH1) can inhibit VEGF and angiogenesis, while heme can specifically degrade BACH1. However, the effect of heme on endothelial cells and ROP remains unknown.
Methods
In this report, we established a model of human microvascular endothelial cells (HMEC-1) induced by 40% hyperoxia to simulate the relatively hyperoxia of phase I ROP. Meanwhile, heme was added to investigate the effects on the growth and viability of HMEC-1. Cell counting kit 8 (CCK8) and 5-ethynyl-2′-deox-yuridine (EDU) methods were used to detect the proliferation ability. Cell scratch test and matrigel matrix glue were used to detect the migration or angiogenesis ability. Western blot and immunofluorescence methods were used to detect the relative protein expression of BACH1 and VEGF.
Results
The proliferation, migration and angiogenesis of HMEC-1 were inhibited under hyperoxia. Moderate heme can promote endothelial cell proliferation, while excessive can inhibit, 20 µM heme could inhibit the expression of BACH1, promote the expression of VEGF, and relieve the inhibition of proliferation, migration and angiogenesis induced by hyperoxia in HMEC-1.
Conclusions
20 µM heme can relieve the inhibitory effects induced by hyperoxia, via the mechanism of promoting VEGF by inhibiting BACH1 in HMEC-1, and maybe a potential medicine for retinal angiogenesis retardation induced by relatively hyperoxia in phase I ROP.
Autistic Traits Are Associated With the Functional Connectivity of Between- but Not Within-attention Systems in the General Population
Authors: Yoshimura Sayaka , Kobayashi Kei , Ueno Tsukasa , Miyagi Takashi , Oishi Naoya , Murai Toshiya , Fujiwara Hironobu 
Published: September 2020

Abstract
Background: Previous studies have demonstrated that individuals with autism spectrum disorder (ASD) exhibit dysfunction in the three attention systems (i.e., Alerting, Orienting, and Executive control) as well as atypical relationships among these systems. Additionally, other studies have reported that individuals with subclinical but high levels of autistic traits show similar attentional tendencies as those observed in ASD. Based on these findings, it was hypothesized that autistic traits would affect the functions and relationships of the three attention systems in a general population. Resting-state functional magnetic resonance imaging (fMRI) was performed in 119 healthy adults to investigate relationships between autistic traits and within- and between-system functional connectivity (FC) among the three attention systems. Twenty-six regions of interest that were defined as components of the three attention systems by a previous task-based fMRI study were examined in terms of within-and between-system FC. Autistic traits were assessed using the Autism-Spectrum Quotient.Result: Correlational analyses revealed significant correlations between autistic traits and between-system FC but not within-system FC. Conclusions: Together with findings of previous studies, these results suggest that high level of autistic traits, even if it is subclinical, affect relationships among the three systems and could possibly induce changes in the efficiency of attention.
Excretable, ultrasmall hexagonal NaGdF4:Yb50% Nanoparticles for bimodal imaging and radiosensitization
Authors: Damasco Jossana Abcede , Ohulchanskyy Tymish Y , Mahajan Supriya D , Chen Guanying , Singh Ajay , Kutscher Hilliard , Huang Haoyuan , Turowski Steven , Spernyak Joseph A , Singh Anurag , Lovell Jonathan F , Seshadri Mukund , Prasad Paras 
Published: September 2020

Abstract
Background
In this study, we report on the synthesis, imaging properties and radiosensitizing properties of β-NaGdF4:Yb50% nanoparticles as a multifunctional theranostic platform. The synthesized nanoparticles act as potent bimodal contrast agents with superior imaging properties compared to existing agents used for magnetic resonance imaging (MRI) and computed tomography (CT). Clonogenic assays demonstrated that these nanoparticles can act as effective radiosensitizers, provided the nanoparticles are taken up intracellularly.
Results
A 2 Gy dose of X-ray induced ~ 20% decrease in colony survival when C6 rat glial cells were incubated with non-targeted nanoparticles (NaGdF4:Yb50%), whereas the same X-ray dose resulted in a ~ 60% decrease in colony survival with targeted nanoparticles conjugated to folic acid (NaGdF4:Yb50%-FA). Intravenous administration of nanoparticles resulted in clearance through urine and feces within a short duration, based on the ex vivo analysis of Gd3+ ions via ICP-MS.
Conclusion
These biocompatible and in vivo clearable ultra-small NaGdF4:Yb50% are promising candidates for further evaluation in image-guided radiotherapy applications.
Spatial Distribution of multielements including technology critical elements in sediments of the Danube River
Authors: Culicov Otilia Ana , Trtic-Petrovic Tatjana , Balvanović Roman , Petković Anđelka , Ražić Slavica 
Published: September 2020

Abstract
Background

The pollution of the second-longest European river (the river Danube) has been under monitoring and focused on various contaminants including metals/metalloids (Hg, As, Ni, Zn, Cu, Cr, Pb, and Cd), personal care products, technical additives, pesticides, pharmaceuticals, etc. Recent studies show that technology critical elements (TCE) – elements with a high supply risk and economic importance – are becoming emerging pollutants due to their wide application in new technologies. According to the European Union Water Framework Directive, sediments are one of the three major sources of river pollution. This study aims to determine major and trace elements including some TCEs in the Danube River sediment. The concentrations of the targeted elements in the surface sediments were discussed in the sense of the effect of building hydropower dam Iron Gate I and increasing the quantity of sediments in the Iron Gate gorge.
Results

The surface sediments were collected on the Danube River-km 1141 to 864 and three tributaries along this waterway. Two samples of deep sediments were used for comparison. Instrumental Neutron Activation Analysis was applied for quantification of 36 elements, with special attention to selected TCE belonging to lanthanides (La, Ce, Nd, Sm, Eu, Gd, Tb, Dy, Tm, and Yb). Spatial distribution is discussed (i) in the total pool of all analyzed elements and (ii) only lanthanides. For better understanding and to highlight a hidden relationship between targeted elements, multivariate statistical techniques (cluster analysis and principal component analysis) were applied to analyze the analytical data and to identify possible pollution sources. The obtained results of the targeted elements in the surface sediments were discussed in relation to the influence of hydropower dam Iron Gates I and the increasing quantity of sediments.
Conclusion

Overall results show increasing concentration of almost all investigated elements in the surface sediments from the Danube River-km 1112 to the dam. Sediment od the River Pek was separated as a location with extreme anthropogenic influence due to close vicinity of the copper mining site.
Improving Smoke Alarm Self-Report Via a Prompted Questionnaire
Authors: Shields Wendy C , Omaki Elise C , McDonald Eileen M , Gielen Andrea C. 
Published: September 2020

Abstract
Background: Previous work has demonstrated discrepancies between self-reported and observed smoke alarm functionality. This study aimed to improve self-report of smoke alarm status. Methods: Participants were asked if they had: 1) any working smoke alarm; and 2) a working smoke alarm on every level of their home (Brief Form). Subsequently, parents answered an Extended Form prompting them to consider each level and how they knew the smoke alarms were functioning. Results: 554 participants completed both forms. On the Brief Form, 96% of participants reported having any smoke alarm and 92% reported having a working alarm on every level. On the Extended Form, 85% of parents reported having any smoke alarm and 73% reported having a working alarm on every level. Conclusion: Prompting residents to consider the presence and functionality of smoke alarms on each level of their home increased the opportunity to provided tailoired communication to improve smoke alarm coverage.
Sarcasm Detection Using Multi-Channel Attention Based BLSTM On News Headline
Authors: Azwar Azika Syahputra , Suharjito  
Published: September 2020

Abstract
Sarcasm is often used to express a negative opinion using positive or intensified positive words in social media. This intentional ambiguity makes sarcasm detection, an important task of sentiment analysis. Detecting a sarcastic tone in natural language hinders the performance of sentiment analysis tasks. The majority of the studies on automatic sarcasm detection emphasize on the use of lexical, syntactic, or pragmatic features that are often unequivocally expressed through figurative literary devices such as words, emoticons, and exclamation marks. In this paper, we introduce a multi-channel attention-based bidirectional long-short memory (MCAB-BLSTM) network to detect sarcastic headline on the news. Multi-channel attention-based bidirectional long-short memory (MCAB-BLSTM) proposed model was evaluated on the news headline dataset, and the results-compared to the CNN-LSTM and Hybrid Neural Network were excellent.
A Surfactant, Oxidant and Inhibitor Compatible Thermo-solvent-tolerant Amylase From a Novel Extrimophilic Bacillus Subtilis Strain Clb-34 Mk443366: Study on Purification and Characterization
Authors: Tiwari Soni , Gaur Rajeeva 
Published: September 2020

Abstract
It is the first time when thermo-tolerant, heavy metal resistance amylase producing strain Bacillus subtilis isolated from soil sample. Amylase was purified 3.8-fold with a specific activity of 11,305.0 U mg-1. The molecular weight of purified amylase was 67 kDa as revealed by SDS-PAGE and activity gel analysis. The amylase was active in broad pH and temperature range of 4.0-11.0 and 35-110°C, respectively, with maxima at pH 7.0 and 85°C temperature. The amylase has Km and Vmax value of 2.181 mg ml-1 and 909.09 µg ml-1min-1, respectively when starch used as substrate. The amylase was not only stable but also its activity enhanced in the presence of n-dodecane, iso-octane, n-decane, xylene, toluene, n-butanol, acetone, and cyclohexane, after prolonged incubation (7 days). Amylase activity and stability was inhibited in the presence of Hg2+, benzene, sodium perborate. The unique property of solvent tolerance and heavy metal resistance proves the potential candidature of this isolate not only for starch liquefaction for food application but also for bioremediation strategies involved in environmental cleanup.
Multi Potent Aromatic Nano Colloid: Synthesis, Characterization and Applications
Authors: S Ranjani , M Salman Al Farzi , p Shruthy Priya , Waseem Mohammad , K Ruckmani , S Hemalatha 
Published: September 2020

Abstract
In this study the aromatic nanocolloids (CANCs) are synthesized using the noble metal silver by using Citronella extract and confirmed through physio chemical analysis. The synthesised CANCs were evaluated for its antimicrobial activity and antibiofilm activity against pathogenic biofilm forming E. coli. In addition, synthesized CANCs were evaluated for the expression of virulent genes encoding AmpC and CTX-M-15. The results confirmed that CANCs showed effective antimicrobial activity through its bacteriostatic, bactericidal and quorum quencher activity and downregulated CTX-M-15 gene. CANCs were validated as alternate to the commercial fungicides to control plant pathogenic fungi such as A. niger MTCC (281), Fusarium graminearum MTCC (2089) and F. udum MTCC (2204). Furthermore, analysis of CANCs on breast cancer (MCF-7) cells under in vitro condition showed that the cytotoxicity of CANCs is dose dependent. Thus, the multifunctional CANCs can be utilized as potential antimicrobial, antifungal and anticancer agent.
Analysis of Clinical Characteristics of Patients with Critical COVID-19
Authors: Zhao Qiquan , Jian Li , Zhou Yuguo , Shang Jiandong , Ning Yan , Peng Fengying , Jia Bei 
Published: September 2020

Abstract
Background: According to the data from the joint investigative team of the WHO and China, the fatality rate in Wuhan is 5.8%and 0.7% in the rest of China. Most of the patients who have died have had chronic diseases or advanced age, and patients with critical COVID-19 have the highest death rate. Patients with critical COVID-19 are the main focus of research on treatment. However, there are few reports on critical COVID-19 patients in China and worldwide. It will help other researchers and clinicians around the world for similar cases.Purpose: Coronavirus disease 2019 (COVID-19) is highly infectious and has a high fatality rate.This paper summarizes and analyses the clinical characteristics of patients with critical COVID-19 to improve clinicians' understanding of this disease. Methods: We selected 16 patients with critical COVID-19 who were treated in the ICU of a grade A hospital in Wuhan from February to March 2020. Then, we collected the clinical data and analysed their general conditions, clinical symptoms, blood tests, CT scans and treatments. Results: Patients with critical COVID-19 had comorbidities (87.5%), and the main symptoms were low or moderate fever (75%), cough and expectoration (68.75%), and multiple lesions in both lungs (100%). 2. Patients with critical COVID-19 were divided into the non-surviving and surviving groups, and the interleukin-6 (IL-6) level and CD4/CD8 ratio were significantly different (P<0.05). 3. On chest CT, multiple patchy lesions were observed in both lungs, mainly as patchy infected lesions, partial consolidation, ground-glass opacities, and interstitial changes. 4. There were 10 patients (62.5%) who survived and 6 (37.5%) who died. Conclusion: Critical COVID-19 is mainly characterized by low or moderate fever, cough and sputum and often occurs in people with chronic disease.Chest CT showed multiple patchy ground-glass opacities and consolidation. For critically ill patients, it is important to monitor interleukin-6 (IL-6) and CD4/CD8 ratio. Early treatment involves thymalfasin, immunoglobulin and other immune-enhancing treatments, and a large dose of ulinastatin can reduce plasma levels of inflammatory factors.
Inferences from dysregulated long non-coding RNA-mediated competing endogenous RNAs in various chemotherapy drugs and evaluation of drug response in breast cancer
Authors: Zhang Ziwen , Li Dongbo , Zhang Han , Qin Qi , Zhang Qingyuan 
Published: September 2020

Abstract
Backgroud: Differences in individual drug response, especially drug resistance, present an obstacle to the treatment of breast cancer (BRCA). Thus, the ability to predict drug response would contribute to developing novel treatment strategies. Accumulating evidence have suggested that tumor molecular profiles and drug response data provide opportunities and challenges for the discovery of new molecular characteristics and mechanisms of drug response in BRCA.
Methods: In the present study, an integrated pipeline was developed to explore drug response-related long non-coding RNA (lncRNA)-mediated competing endogenous RNAs (ceRNAs) motifs in BRCA.
Results: Drug response-specific ceRNAs indicated that lncRNAs play an essential role in various drug treatments for BRCA. Several key drug-resistant and -sensitive dysregulated ceRNAs were identified in Adriamycin, Cytoxan, and Tamoxifen. The interactions in these ceRNAs showed strong correlations in BRCA. Most drug response-related dysregulated ceRNAs were only present in one kind of drug. A number of drug response-related ceRNAs presented diverse dysregulation patterns. We also extracted some key drug response-related lncRNAs, such as HCP5 and FAM182A. These lncRNAs were associated with certain cancer hallmarks and survival in BRCA.
Conclusions: Ultimately, understanding the underlying lncRNA-mediated ceRNAs in drug responses will facilitate improved individual reactions to chemotherapy and overall outcomes of BRCA treatment.
Determinants of Growth Monitoring and Promotion Service Utilization Among Children 0-23 Months of Age in Northern Ethiopia: Unmatched Case Control Study
Authors: Dagne Samuel , Aliyu Jemal , Menber Yonatan , Wassihun Yosef , Fentahun Netsanet 
Published: September 2020

Abstract
Back ground: Child malnutrition is one of the world’s public health problems. Promotion of child growth and development is one of the health priorities to control child mortality and poverty reduction. One of the strategies to promote child health is growth monitoring and promotion services. But, there is limited information on determinants of Growth Monitoring and Promotion service utilization. Objective: To identify determinants of growth monitoring and promotion (GMP) service utilization among children 0-23 months of age in Legambo district, South Wollo zone, Northern Ethiopia, 2020.Methods: Community based un-matched case control study was conducted on 363 (91 cases and 272 controls) study participants from March 15 to April 15, 2020. . Multi-stage sampling technique was employed to select the study participants. . Data were collected by using interviewer administered questionnaire and entered in to Epi data and exported to SPSS version 23 for analysis. Bivariable and multivariable logistic regressions were performed and adjusted odds ratio with 95% confidence intervals was estimated to identify determinants of GMP service utilization.Results: Good maternal knowledge (AOR) = 2.42; 95% CI: 1.23, 4.75), mother with favorable attitude (AOR = 2.45; 95% CI; 1.20, 4.98), counseling on GMP (AOR = 2.34; 95% CI; 1.19, 4.56), Attending ante natal care services (AOR = 2.46; 95% CI: 1.18, 5.16), index child age 12-17 months(AOR = 3.45; 95% CI: 1.26, 9.41) and 18-23 months (AOR = 4.38; 95% CI: 1.53, 12.49), and short distance to health facilities (AOR = 4.53; 95% CI; 1.99, 10.28) were determinants of GMP service utilization.Conclusions and recommendation: Index child age, good knowledge, favorable attitude, attending ante natal care services, receiving nutritional counseling and short distance to health facility were determinants of GMP service utilization. Nutritional interventions should emphasis nutritional counseling, utilization of ANC service and accessibility of growth monitoring and promotion service to improve growth monitoring and promotion service utilization and reduce malnutrition in Ethiopia
Chlorella Vulgaris Biomass Supplementation Increased the Abundance of Hepatic Antioxidant Proteins in Relation to Oxidative Stress Reduction in Rabbits
Authors: Sikiru Akeem Babatunde , Arunachalam Arangasamy , Egena Stephen Sunday Acheneje , Veerasamy Sejian , Reddy Ippala Janardhan , Bhatta Raghavendra 
Published: September 2020

Abstract
Background Chlorella vulgaris is a unicellular microalga that is rich in antioxidant, its supplementation has been reported to reduce oxidative stress via upregulations of antioxidant genes. However, there are scarce reports on its effect on antioxidant protein expressions in rabbits – a situation which necessitate an untargeted proteomic profile analysis due to its supplementation. This is because untargeted proteomics profiling is an approach suitable for assessing the effectiveness of genes code translation into polypeptide chains folded into functional proteins used for specific sub-cellular or extracellular physiological activities. It remains one of the comparative avenues for evaluating the efficacies of drugs and nutraceutical agents including antioxidants. In this study, the antioxidant efficacy of a microalga Chlorella vulgaris was evaluated at molecular levels using its hepatic protein expression in rabbit models. Results After 120 days of the microalga supplementation, protein was extracted from liver of the rabbits for untargeted proteomics profiling using LC-MS/Orbitrap Fusion Tribrid™ peptides quantifier and sequencer. There were five-hundred and eleven (511) proteins identified; and among the proteins, 191 were specific to the control group while 186 were specific to the Treatment group; and 134 were common to both groups. Independent samples t-test of the protein abundance indicated that there was a significant difference (p = 0.01) between the treatment and the control groups. There was also a significant reduction in the malondialdehyde concentrations (p = 0.01), higher total antioxidant capacities (p = 0.002), and increased antioxidant enzyme activities (p = 0.05) between the treatment and control groups.Conclusion The study concluded that one of the molecular mechanisms associated with Chlorella vulgaris intake reduction of the hepatic oxidative stress is increased abundances of antioxidant proteins and reduction of the lipid peroxidation and these led to a suggestion that the microalga is a potent antioxidant agent suitable for protecting against oxidative stress in rabbits and other domestic food producing animals.