bluuebunny/crossref_metadata_2025_split huggingface dataset:
Novel role of hesperidin improve obesity in HFD mice by modulating the composition of the gut microbiota
Authors: Liu Zhihua , Liu Ting , Lei Chao , Song Weiqi , Fang Rong , Chen Hui , Li Chen , Li Xiaomei , Liang Xue , Huang Qinghong , Ti Huihui , Sun Ning , Ma Yanlei 
Published: February 2020

Abstract
Background: Hesperidin is a plant-derived dihydroflavone derivative with multiple pharmacological functions. Obesity is associated with low-grade chronic inflammation and intestinal dysbiosis. We examined the possibility that hesperidin may prevent diet-induced obesity by modulating the composition of the gut microbiota. High-fat diet (HFD)-fed mice were treated with hesperidin. Its effects on the gut microbiota were assessed by horizontal faecal microbiota transplantation (FMT) and 16S rRNA gene amplicon sequencing-based microbiota analysis.
Results: Gut microbiota analysis revealed that hesperidin selectively promoted the growth of beneficial Lactobacillus salivarius and harmful Staphylococcus sciuri and Desulfovibrio C21_c20 and inhibited that of beneficial Bifidobacterium pseudolongum and Mucispirillumschaedleri and harmful Helicobacter ganmani and Helicobacter hepaticus . However, hesperidin reversed obesity and inflammation and improved gut integrity in HFD-fed mice. The anti-obesity effects and hesperidin-modulated Lactobacillus salivarius, Desulfovibrio C21_c20, Mucispirillumschaedleri and Helicobacter hepaticus were transmissible via horizontal faeces transfer from hesperidin-treated mice to HFD-fed mice.
Conclusions: Hesperidin plays a dual role in both beneficial and harmful microbes. However, its overall effects reduce body weight and reverse HFD-related disorders in HFD-fed mice.
A pioneer usage of nanogold sandwich ELISA in the detection of hydatid antigen in humans as a tool for improvement of serodiagnostic testing.
Authors: Elawamy Waleed E , Rashed Samia M , Nassr Mona E , Shalash Ibrahim R A , Ali Nagwa S , Elghanam Amera S , Nageeb Marwa M 
Published: February 2020

Abstract
The authors have withdrawn this preprint from Research Square.
Predictors of Poor Postoperative Outcomes in Pediatric Surgery Patients in Rural Sub-Saharan Africa
Authors: Peiffer Sarah , Ssentongo Anna E. , Keeney Laura , Amponsah-Manu Forster , Yeboako Richard , Ofosu-Akromah Richard , Arkorful Temitope Ebenezer , Agyemang Eric , Tsai Anthony , Oh John , Ssentongo Paddy 
Published: February 2020

Abstract
Background/Purpose Perioperative complications cause significant pediatric morbidity and mortality in low- and lower middle -income countries. This study investigates factors associated with prolonged length of stay, 90-day readmission and in-hospital mortality among pediatric patients in eastern Ghana. Methods This is a retrospective review of perioperative morbidity and mortality in children <18 years at Eastern Regional Hospital (ERH) in Koforidua, Ghana. All pediatric surgeries performed between January 2015 and December 2017 were included in this study and secondary analysis was performed. Results We analyzed 468 patients <18 years of age with a median length of stay (LOS) of 3 days. The readmission and in-hospital mortality rates were 138 and 17 per 1000 patients, respectively. The most common procedures were herniorrhaphy (19%) and appendectomy (15%). Major procedures, gastrointestinal surgery, surgical trauma, surgical infection and having insurance were significantly associated with prolonged LOS. Age and male gender were significantly associated with in-hospital mortality. Malaria was significantly associated with 90-day readmission. Conclusions Malaria infection is a significant and actionable risk factor for readmission in the pediatric surgical population in sub-Saharan Africa. Preventing readmission in patients with malaria could reduce readmission rates by 74%, leading to potential cost-savings and reductions in morbidity.
Does the self-employment status affect the rural migrants’ health in China: the transmission channel of social health insurance?
Authors: Wang Zicheng , Wu Qiushi , Ming Juan 
Published: February 2020

Abstract
Background There are growing attentions paid on rural migrants’ health. Previous studies found that rural migrants are more exposed to disease risks. The present study aims to explore the causal effect of self-employment behavior on the rural migrants’ health status, two issues are addressed to discuss: Does self-employment status affect the health of rural migrants? What is the potential mechanism linking the self-employment behavior and health status? Methods The dataset from the 2017 National Migrants Population Dynamic Monitoring Survey (NMPDMS-2017) is applied to explore the causal effect; the Logit regression is performed to make baseline estimation, while the IV-LPM estimation is applied to correct the endogeneity of self-employment. Additionally, the Logit regression is conducted to explore the transmission channel. Results The self-employed are more susceptible to sub-health status (OR= 1.042; 95% CI= 1.001, 1.084) and chronic disease (OR= 1.394; 95% CI= 1.317, 1.476), even when correcting the endogeneity, the causal effect estimation also demonstrates that the self-employed are more vulnerable to suffer sub-health status (Coefficient= 0.067; 95% CI= 0.050, 0.084) and chronic disease (Coefficient= 0.020; 95% CI= 0.008, 0.032). The self-employed are less likely to participate in social health insurance (OR= 0.057; 95% CI= 0.053, 0.061). Conclusion The self-employed are more likely to suffer sub-health status and chronic disease, the self-employment behavior take harmful effect on the rural migrants’ health. Social health insurance may serve as transmission channel linking self-employment and rural migrants’ health, that is, the self-employed are less prone to participate in the urban health insurance program, which induce to an insufficient health service to maintain health.
WITHDRAWN: Correlates of highly caffeinated beverage consumption among Korean adolescents
Authors: Kwak Ho-Kyung , Sa Jaesin , Choe Siyoung , Chaput Jean-Philippe , Chung Joon , Cummings Gayle , Lee Jounghee 
Published: February 2020

Abstract
Background : Adolescents in South Korea may be particularly vulnerable to highly caffeinated beverage consumption, which may lead to psychological and physiological problems. However, correlates of highly caffeinated beverage consumption have not been rigorously examined for Korean adolescents. This study investigated socio-demographic and behavioral factors related to highly caffeinated beverage consumption, using a nationally representative sample of Korean adolescents.

Methods : We used 267,907 middle and high school students from the Korea Youth Risk Behavior Web-based Survey collected from year 2014 to 2017 for the present cross-sectional study. We employed multiple logistic regression to examine the associations between highly caffeinated beverage consumption and socio-demographic and behavioral factors.

Results : Highly caffeinated beverage consumption was higher in 2017 than 2014 (23.9% vs. 12.0%) among Korean adolescents. Drinkers of highly caffeinated beverages were more likely to be boys, to be overweight or obese, to overestimate their body weight, to drink soda > 1 time per week, and to drink sweetened beverages > 1 time per week ( p s < .05). Additionally, drinkers of highly caffeinated beverages were more likely to feel stressed, to feel sadness and depression, to seriously consider suicide, and to attempt suicide during last 12 months ( p s < .05). Among male participants, drinkers of highly caffeinated beverages were less likely to meet the recommended 8 to 10 hours of sleep per night ( p < .05).

Conclusions : Highly caffeinated beverage consumption is a potential health concern, as drinkers of highly caffeinated beverages were more likely to report health and behavioral problems. New strategies are needed to effectively curb highly caffeinated beverage consumption in Korea.
Buccolingual Inclination of Canine and First and Second Molar Teeth and The Curve of Wilson in Different Sagittal Skeletal Patterns of Adults Using Cone-Beam Computed Tomography
Authors: Golshah Amin , Rezae Navid , Heshmati Sara 
Published: February 2020

Abstract
Background: This study aimed to assess the buccolingual inclination of canine and first and second molar teeth and the curve of Wilson in different sagittal skeletal patterns in untreated adults using cone-beam computed tomography (CBCT). Methods: Sixty-six CBCT scans of adults (mean age: 28.74±5.25 years) were evaluated in this cross-sectional study. The images were standardized using the Frankfurt horizontal plane and the interorbital line. The sagittal skeletal pattern was determined using the ANB angle and Wits appraisal. Inclination angles were measured by NNT Viewer and Mimics software. The curve of Wilson was measured by connecting the tips of mesiobuccal and mesiolingual cusps of maxillary first and second molars along the buccal groove and measuring the formed angle. Data were analyzed using ANOVA. Results: The intraobserver agreement was 0.969. The mean inclination of maxillary first and second molars in class I and III patients was significantly higher than that in class II patients (P<0.05). The mean inclination of mandibular first and second molars in class II patients was significantly higher than that in class I and III patients (P<0.05). The difference in inclination of maxillary and mandibular canine teeth was not significant (P>0.05). The mean curve of Wilson in second molars of class II patients was significantly higher than that in class I patients (P<0.05). Conclusion: In different sagittal skeletal patterns, a compensatory relationship exists between the opposing teeth, which along with the standards of crowns, can be used to determine the appropriate position of teeth in dental arch.
Drug users’ perceptions, demands, and suggestions for non-occupational post-exposure prophylaxis (nPEP) services in China: a qualitative study
Authors: Jiao Kedi , Li Haochu , Zhang Dapeng , Jiang Zhenxia , Lin Yuxi , Liu Xueyuan , Xu Hengmin , Yan Xuemei , Tang Haoqing , Ma Wei 
Published: February 2020

Abstract
Background Drug users are regarded as a high-risk population of HIV infection. Non-occupational post-exposure prophylaxis (nPEP) is internationally regarded as an effective biomedical prevention against HIV but still a small-scale pilot project in China at present. The aim of this study was to understand drug users’ perceptions, demands and suggestions for nPEP service in China. Methods Nineteen eligible drug users were recruited in this qualitative study. Semi-structured in-depth interviews were conducted by trained interviewers. All of the interviews were audio-taped, transcribed verbatim and analyzed using thematic framework analysis. Results Most of participants had not heard of nPEP and none of them had used this service. After being informed basic knowledge of nPEP, about half of the participants reported willingness to use it. The facilitators included scare of HIV infection and regarding nPEP as a remedial measure in emergency. The barriers included low willingness for fatigue and lethargy after taking drugs, high price and side effects of nPEP medication, long course of nPEP treatment, and fear of privacy disclosure. Most of the participants thought the availability of nPEP would not increase the occurrence of unprotected intercourse and supported the routinization of nPEP services. Most participants chose social media for nPEP propaganda and pharmacies for providing nPEP. Conclusions It is imperative to strengthen propaganda and education of nPEP among drug users, and to encourage them to proactively use nPEP after exposure. Price regulation, side effect management and privacy protection need to be managed well when nPEP is routinized.
New heritable ATRX mutation identified by whole exome sequencing and review
Authors: Shahbazi Zahra , Rostami Golale , Hamid Mohammad 
Published: February 2020

Abstract
Background

ATRX gene encodes a member of the SWI2/SNF2 family of proteins that may act as a transcriptional factor and plays a significant role in the epigenetic regulation of gene expression. The mutations in the ATRX gene have been shown to cause two types of disorders: inherited mutations lead to alpha thalassemia X-linked mental retardation (ATR-X) syndrome and acquired somatic mutations cause alpha thalassemia myelodysplastic syndrome (ATMDS). Here we report a case of ATRX gene mutation without completely features of ATR-X or ATMDS syndromes. Moreover we review previous reports of ATRX gene mutations in both ATR-X syndrome and ATMDS.

Methods

Patient was a 29-year-old male of Arab ancestry with normocytic anemia, low mean corpuscular hemoglobin (MCH), haematocrit percent and red blood cell (RBC) count. He had no sign of mental retardation and facial dysmorphism. The whole exome sequencing method was used to find the disease-causing variants. Moreover we searched HGMD, Ensembl, OMIM and COSMIC databanks for all mutation reported in ATRX gene so far, and Pubmed, WOS, Science direct and springer link for articles that reported ATRX gene mutations.

Results

We identified a hemizygous missense ATRX gene mutation ( ATRX, c.2388A>C, p. K796N) as a new disease-causing variant in the patient.

Conclusion

According to previous findings, inherited ATRX mutations are associated with a broad spectrum of clinical presentations. Therefore a person with a mild α-thalassemia phenotype may also have mutation in ATRX gene. Accordingly, it is critical for geneticist and physicians to increase awareness in molecular diagnosis of α-thalassemia patients.
A generic emergency protocol for patients with inborn errors of metabolism causing fasting intolerance: a retrospective, single-center chart review of 128 patients
Authors: Hoogeveen Irene J. , Amerongen Tessa van , Weynschenk Frouke J. , Lubout Charlotte M. A. , Boer Foekje de , Fokkert-Wilts Marieke J. , Rodenburg Iris L. , Dam Esther van , Grünert Sarah C. , Martinelli Diego , Spronsen Francjan J. van , Derks Terry 
Published: February 2020

Abstract
Background - Patients with inborn errors of metabolism causing fasting intolerance are at risk of acute metabolic decompensations. Disease specific emergency protocols are widely available, but long-term data on safety and efficacy outcomes are lacking. We hypothesized that a generic emergency protocol can be safe and effective in patients with inborn errors of metabolism causing fasting intolerance. Results - We retrospectively evaluated our generic emergency protocol in 128 patients with a hepatic glycogen storage disease or fatty acid oxidation defect between February 1, 2014 and April 24, 2019. In total, 127 hospital admissions were documented in 54 out of 128 patients (42%). Hypoglycemia (glucose concentration <3.9 mmol/l) was reported in 15% of admissions. Hypoglycemia at admission was uncommon in patients with ketotic glycogen storage disease and verbal patients with a fatty acid oxidation defect. Convulsions, coma or death were not reported. Conclusions - Generic emergency protocols can be safe and effective for home management by the caregivers and the first hour in-hospital management of metabolic emergencies in patients with hepatic glycogen storage disease and medium-chain Acyl CoA dehydrogenase deficiency.
Bone collagen particles combined with hUC-MSCs to repair alveolar cleft in rabbits
Authors: Sun Xue-Cheng , Wang Hu , Li Jian-Hui , Zhang Dan , Ma Xu , Xia Hong-Fei 
Published: February 2020

Abstract
Background: Alveolar cleft is a kind of cleft lip and palate, which seriously affects the physical and mental health of patients. In this study, a similar model of human alveolar cleft phenotype was established in rabbits to evaluate the effect of bone collagen particles combined with human umbilical cord mesenchymal stem cells (hUC-MSCs) on the repair of alveolar cleft. Materials &Methods : In this study, 24 adult Japanese white rabbits (JWRs) were selected and randomly divided into 4 groups. Including normal group, control group, materials group and MSCs group. The model of alveolar cleft was established by removing the incisors on the left side of the upper jaw. The normal group did not receive any treatment. In the control group, the incisors were removed and sutured directly. In the material group, the incisor were removed, then filled with bone collagen particles, and finally sutured. In the MSCs group, the incisors were first removed, then filled with bone collagen granules incubated by hUC-MSCs, and then stitched. Blood biochemical analysis was performed 3 months after the operation. Skull tissues were collected for gross observation, and micro-focus computerized tomography (micro-CT) analysis. Paraffin sections were prepared for histological and immunohistochemical staining. Results: The bone collagen particles and hUC-MSCs are not biotoxic and can promote alvenlus regeneration. Bone collagen particles combined with hUC-MSCs were much better than those used alone in inducing bone repair and regeneration. Conclusions: HUC-MSCs can be used as a bone generation inducer combined with bone materials for bone regeneration and repair. Keywords: alveolar cleft，Bone collagen particle, hUC-MSCs (human umbilical cord mesenchymal stem cells),micro-focus computerized tomography (micro-CT)
Prediction and analysis of human-herpes simplex virus type 1 protein-protein interactions by integrating multiple methods
Authors: Lian Xianyi , Yang Xiaodi , Shao Jiqi , Hou Fujun , Yang Shiping , Pan Dongli , Zhang Ziding 
Published: February 2020

Abstract
Background: Herpes simplex virus type 1 (HSV-1) is a ubiquitous infectious pathogen that widely affects human health. To decipher the complicated human-HSV-1 interactions, a comprehensive protein-protein interaction (PPI) network between human and HSV-1 is highly demanded. Results: To complement experimental identification of human-HSV-1 PPIs, we developed an integrative strategy to predict proteome-wide PPIs between human and HSV-1. For each human-HSV-1 protein pair, four popular PPI inference methods, including interolog mapping, the domain-domain interaction-based method, domain-motif interaction-based method and machine learning-based method, were optimally implemented and employed to generate four interaction probability scores, and then these four scores were further integrated into a final probability score. As a result, a comprehensive high-confidence PPI interaction network between human and HSV-1 was established, which covers 10,432 interactions between 4,546 human proteins and 72 HSV-1 proteins. Functional and network analyses of the HSV-1 targeting proteins in the context of human interactome can recapitulate the known knowledge regarding the HSV-1 replication cycle, supporting the overall reliability of the predicted PPI network. Considering that HSV-1 infections are implicated in encephalitis and neurodegenerative diseases, we focused on exploring the biological significance of brain-specific human-HSV-1 PPIs. In particular, the predicted interactions between HSV-1 proteins and Alzheimer's-disease-related proteins were intensively investigated. Conclusions: The current work can provide testable hypotheses to assist mechanistic understanding of the human-HSV-1 relationship as well as the discovery of anti-HSV-1 pharmaceutical targets. To make the predicted PPI network and the datasets freely accessible to the scientific community, a user-friendly database browser has been released at http://www.zzdlab.com/HintHSV/index.php. Keywords: Human-virus interaction, Protein-protein interaction, Prediction, Herpes simplex virus type 1, Alzheimer's disease
Late complications of radiosurgery for cerebral arteriovenous malformations: report of 5 cases of chronic encapsulated intracerebral hematomas and review of the literature
Authors: Finitsis Stephanos , Anxionnat René , Bernier Valerie , Buccheit Isabelle , Klein Olivier , Bracard Serge 
Published: February 2020

Abstract
Background: Chronic encapsulated intracerebral hematomas (CEIHs) are a rare, late complication of radiosurgery for intracranial AVM. We present 5 cases treated mostly by surgical excision and review the literature. Methods: Patients (age 39, 42, 36, 31, 62) presented with headache, paresthesia, hemiparesis or were asymptomatic. CEIHs presented 10 to 13 years (median 12 years) post radiosurgery. Three patients had demonstrated early radiation induced changes post radiosurgery. Angiographic cure, assessed with DSA, was present in all cases except 1 case with a small nidus remnant. MRI demonstrated mixed lesions with a solid enhancing part, organized hematoma and extensive surrounding edema while three cases had also a cystic component. Results: Excision of the CEIHs with complete or partial removal of the capsule was perfomed in 4 patients and resulted in marked clinical improvement. On patient was managed conservatively with administration of steroids as surgery was judged excessively hazardous with eventual stabilization of his symptoms. Conclusions: CEIHs are rare, late complications of radiosurgery for cranial AVM. They may be asymptomatic or provoke symptoms and may be preceded by early radiation induced changes. Complete removal of CEIHS is an effective treatment. Because of the long latency period of CEIHs, patients who had radiosurgery for brain AVMs should be followed by MRI at least 10 years even after complete obliteration. Keywords: Arteriovenous Malformation, Radiosurgery, Complication, Chronic encapsulated intracerebral hematoma
Adiponectin Improves Therapeutic Efficacy of Mesenchymal Stem Cells by Enhancing Their Engraftment and Survival in Peri-infarct Myocardium Through AMPK Pathway
Authors: Tian Xiaqiu , Qian Xiao-Song , Wang Hong , Yang Yue-Jin 
Published: February 2020

Abstract
Background: Poor viability of transplanted mesenchymal stem cells (MSCs)within the ischemic heart has limited their therapeutic potential for cardiac repair. We have recently demonstrated that adiponectin (APN) inhibits the apoptosis of MSCs under hypoxia and serum-deprivation conditionsin vitro. This study investigated whether APN could promote the survival of MSCs in vivoand further contribute to cardiac repair after acute myocardial infarction (AMI), via the adenosine monophosphate-activated protein kinase(AMPK)pathway. Methods: Rats were randomized into six groups: the Sham, AMI control, and 4 other groups that were subjected to AMI followed by treatment with MSCs, APN, APN + MSCs and APN + MSCs + AMPK inhibitor. MSCs labeled with CM-Dil were injected through the jugular vein in 24 hours post AMI. At 4-week after AMI, engraftment of MSCs to the peri-infarct myocardium was evaluated. Cardiac function was assessed using echocardiography and left heart catheterization. Apoptosis and fibrosis were measured with TUNEL and Masson’s trichrome staining. H&E staining and immunohistochemistry against CD 68 and CD 206 were performed to assess the infiltration of inflammatory cells. Expressions of inflammatory cytokines were determined with ELISA. Immunostaining against smooth muscle cell marker α-smooth-muscle actin (α-SMA) and endothelial cell marker CD31 antibodies were performed to assess arteriogenesis and angiogenesis. Results: APN treatment significantly enhanced the engraftment and survival rate of transplanted MSCs accompanied by markedly improved cardiac function and decreased infarct size at 4-week after AMI. Combined administration of APN and MSCs markedly suppressed inflammatory response, specifically promoted shift of infiltrated macrophages to anti-inflammatory phenotype.Combined administration of APN and MSCs also significantly inhibited cardiomyocytes apoptosis, while increased arteriogenesis and angiogenesis in the peri-infarct myocardium when compared with MSCs transplantation alone. These protective effects of APN were associated with AMPK phosphorylation, which were almost completely reversed by AMPK pathway inhibitor. Conclusions: Our results demonstrated that APN could improve the survival and therapeutic efficacy of transplanted MSCs after AMI through AMPK activation.Our study suggests the potential application of APN for improvement of stem cell-based heart repair and regeneration.