bluuebunny/crossref_metadata_2025_split huggingface dataset:
Far Infrared Technology (FIT) Therapy Patches, Protects from Inflammation, Oxidative Stress and Promotes Cellular Vitality
Authors: Pastore Donatella , Pacifici Francesca , Ciao Giampaolo , Bedin Valentina , Pasquantonio Guido , Della-Morte David 
Published: April 2020

Background:
It is known from the most recent literature that far-infrared (FIR) radiations promote a
broad spectrum of therapeutic benefits for cells and tissues.

Objective:
To identify molecular mechanisms by which FIT patches, as a far infrared technology, protects against
damage caused by inflammatory process and oxidative stress.

Methods:
Endothelial cells (HUVEC, Human Umbilical Vein Endothelial Cells) were used as in vitro experimental
model. HUVEC were stimulated with a pro-inflammatory cytokine, TNF-α, or hydrogen peroxide (H2O2) to
induce oxidative stress. As markers of inflammation were evaluated: VCAM1 (Vascular Cell Adhesion Molecule
1), ICAM1 (Intercellular Adhesion Molecule 1) and E-Selectin by Western Blot analysis. Oxidative stress was
assessed by cytofluorimetric analysis. The experiments were performed on control cells (no patch) or in cells
treated with the FIT infrared technology applied on the basis of the culture plate.

Results:
HUVEC stimulated with TNF-α and treated with FIT patches had significant reduction of the expression
of VCAM1, ICAM1 and E-Selectin (p<0.05). HUVEC stimulated with H2O2 and treated with FIT patches were
significantly protected from oxidative stress (p <0.01) when compared to control cells. We measured cell viability
and proliferation in HUVEC and HEK-293 (Human embryonic kidney cells) cells by MTT assay. HEK-293 and
HUVEC treated with FIT patches showed a significantly higher percentage of basal vitality compared to control
cells (p<0.0001 for HEK-293, p<0.05 for HUVEC).

Conclusion:
FIT therapy patches - infrared technology, through these protective mechanisms, could be used in all
pathologies where an increase in inflammation, oxidative stress and degenerative state are present.
Analysis of Inter-Chromosomal Distribution of Disease-Related Genes in Human Genome
Authors: Sun Xiaochao , Yang Bin , Zhang Qunye 
Published: April 2020

:
Many studies have shown that the spatial distribution of genes within a single chromosome
exhibits distinct patterns. However, little is known about the characteristics of inter-chromosomal distribution
of genes (including protein-coding genes, processed transcripts and pseudogenes) in different
genomes. In this study, we explored these issues using the available genomic data of both human and
model organisms. Moreover, we also analyzed the distribution pattern of protein-coding genes that
have been associated with 14 common diseases and the insert/deletion mutations and single nucleotide
polymorphisms detected by whole genome sequencing in an acute promyelocyte leukemia patient. We
obtained the following novel findings. Firstly, inter-chromosomal distribution of genes displays a nonstochastic
pattern and the gene densities in different chromosomes are heterogeneous. This kind of
heterogeneity is observed in genomes of both lower and higher species. Secondly, protein-coding
genes involved in certain biological processes tend to be enriched in one or a few chromosomes. Our
findings have added new insights into our understanding of the spatial distribution of genome and disease-
related genes across chromosomes. These results could be useful in improving the efficiency of
disease-associated gene screening studies by targeting specific chromosomes.
The Key Role of Akt Protein Kinase in Metabolic-Inflammatory Pathways Cross-Talk: TNF-α Down-Regulation and Improving of Insulin Resistance in HepG2 Cell Line
Authors: Alipourfard Iraj , Bakhtiyari Salar , Gheysarzadeh Ali , Di Renzo Laura , De Lorenzo Antonio , Mikeladze David , Khamoushi Atefeh 
Published: April 2020

Background:
Elevation of plasma free fatty acids as a principal aspect of
type 2 diabetes maintains etiologically insulin insensitivity in target cells. TNF-α inhibitory
effects on key insulin signaling pathway elements remain to be verified in insulinresistant
hepatic cells. Thus, TNF-α knockdown effects on the key elements of insulin
signaling were investigated in the palmitate-induced insulin-resistant hepatocytes. The
Akt serine kinase, a key protein of the insulin signaling pathway, phosphorylation was
monitored to understand the TNF-α effect on probable enhancing of insulin resistance.

Methods:
Insulin-resistant HepG2 cells were produced using 0.5 mM palmitate
treatment and shRNA-mediated TNF-α gene knockdown and its down-regulation
confirmed using ELISA technique. Western blotting analysis was used to assess the Akt
protein phosphorylation status.

Results:
Palmitate-induced insulin resistance caused TNF-α protein overexpression
1.2-, 2.78, and 2.25- fold as compared to the control cells at post-treatment times of 8 h,
16 h, and 24 h, respectively. In the presence of palmitate, TNF-α expression showed
around 30% reduction in TNF-α knockdown cells as compared to normal cells. In the
TNF-α down-regulated cell, Akt phosphorylation was approximately 62% more than
control cells after treatment with 100 nM insulin in conjugation with 0.5 mM palmitate.

Conclusions:
The obtained data demonstrated that TNF-α protein expression reduction
improved insulin-stimulated Akt phosphorylation in the HepG2 cells and decreased lipidinduced
insulin resistance of the diabetic hepatocytes.
Vaccine Plasmid Topology Monitoring by Capillary Gel Electrophoresis
Authors: Cook K. Steven , Luo Jane , Guttman Andras , Thompson Lawrence 
Published: April 2020

Background:
Plasmid DNA has been widely used in vaccination as well as in
cell and gene therapy. It exists in multiple isoforms, including supercoiled, nicked or
open circular and linear forms. Regulatory agencies recommend having more than 80%
of the supercoiled isoform for the bulk release of plasmid products; thus, it should be
analyzed accordingly.

Methods and Results:
The traditional analysis method for plasmid DNA is agarose gel
electrophoresis. However, due to time-consuming manual sample loading, visualization,
and data analysis, it has limitations in obtaining consistently quantitative results. In this
short communication, we introduced a fast, sensitive, and robust plasmid analysis
method using capillary gel electrophoresis with laser-induced fluorescence detection
(CGE-LIF). CGE-LIF analysis of the supercoiled isoform and its open circular
counterpart was completed in 20 minutes with excellent sensitivity by using a common
fluorescent DNA binding dye. The advantage of the method was demonstrated by the
purity analysis of two large plasmids (7 kb and 10 kb). The fully automated sample
loading, separation and data analysis featured enhanced assay repeatability and ease of
quantitation over agarose gel electrophoresis.

Conclusion:
As a worked example, analysis of plasmid samples treated at elevated
temperature during an accelerated stability test also demonstrated the applicability of
CGE-LIF to monitor plasmid topology and possible degradation.
Evaluation of Antidiabetic Activity of Rosmarinus officinalis var. prostratus Growing in Syria in Alloxan Diabetic Rats
Authors: Malek Azhar , M. Sadaka M. Waleed , Hamo Sameh , Al-Mahbashi Hassan M. 
Published: April 2020

Background:
The genus of Rosmarinus officinalis (Rosemary) is found in many parts of the world.
Traditionally, Rosemary has been used in traditional medicine due to its therapeutic virtues and its ability to
treat some diseases.
Rosmarinus officinalis var. prostrates grows in the Al Kalamoon region of Syria but only a limited amount of
scientific research on the medicinal uses of this plant has been done. This study focused on the medicinally
active substances found in ethanolic extract of the leaves and flowers of this plant and their antidiabetic
effect in alloxan-induced diabetic rats. First, an acute toxicity study was carried out to detect the optimal
dose of the Rosmarinus officinalis var. prostrates extract. Then, the effect of the optimal dose of 300mg/kg
was measured over 36 days.

Method:
During the study, diabetic rats were treated with different doses of extract (100, 200, 300, 400, 500,
and 600 mg/kg). The percentage of fasting blood glucose (FBG) was measured immediately after
administration of the extract and at 2h, 4h and 8h after administration. The dose of 300mg/kg was then used
in the second part of the study, which involved four groups of rats: a healthy group, a diabetic group without
treatment (the control group), a diabetic group treated with 300mg/kg of extract, and a diabetic group treated
by metformin (50mg/kg). The treatment continued for 36 days.

Results:
The results showed that the extract was rich with flavonoids, phenols and tannins. Levels of FBG
and DPP-4 were significantly lower in the extract-treated group in comparison with the control group;
however, the level of insulin was significantly elevated in the extract-treated group compared to the control
group. This effect may be caused by two factors. First, the antioxidant effects of flavonoids which protect
pancreatic beta cells from damage caused by alloxan, supports regeneration of pancreatic beta cells, and
therefore insulin production. Second, the inhibition of DPP-4 activity, which in turn leads to increased
secretion of insulin.

Conclusion:
The ethanolic extract of Rosmarinus officinalis var. prostrates has an antidiabetic effect.
A Review on Sustainable Development of Geological Exploration Technology and Risk Management
Authors: Liang Rui , Tang Panke , Xiong Guoxiang , Liu Ziyang , Yu Dandan 
Published: April 2020

Background::
Environmental protection is increasingly being emphasized upon, and
higher requirements are put forward for the development of the geological prospecting industry.
Researchers have been trying their best to explore the mineral resources in the deep part of the earth
and the areas with poor working conditions, and avoid the damage of mining development to the environment,
so as to achieve the goal of green prospecting and sustainable geological exploration.

Methods:
This greatly increases the uncertainty of ore prospecting and requires the traditional
technical means to be improved. With the great progress of computer hardware and software, the
application of geophysical exploration, geochemical exploration, remote sensing, and other methods
that have little damage to the environment, have been improved. A reasonable technology mix
can be used more efficiently and explored in a cost-effective way.

Conclusion:
According to the requirements of green and sustainable exploration, geophysical exploration,
geochemical exploration and remote sensing can acquire surface data of a certain area
more quickly with less economic cost and lower environmental impact, so as to provide decision
for the next step of prospecting and avoid the risk to a greater extent. The prospecting information
obtained by the three methods cannot directly control mine. The specific method selection and
combination need to consider the actual demand, in order to complete the geological exploration
work better and with rational use of funds.
HPTLC Analysis with the Effect ofBacopa monnieri, Evolvulus alsinoidesandTinospora cordifoliaagainst Scopolamine-Induced Amnesic Rats
Authors: Gupta Avneet , Singh Manish P. , Sisodia Siddhraj S. 
Published: April 2020

Background and Objective:Bacopa monnieri, Evolvulus alsinoides, and Tinospora cordifolia are well known herbal Ayurvedic medicinal plants with a memory enhancing property. The aim of this study was HPTLC analysis for evaluating the effect of ethanolic extract of Bacopa monnieri (BME), Evolvulus alsinoides (EAE) and Tinospora cordifolia (TCE) alone and in combination of equal proportion (CEPs) against scopolamine-induced amnesic rats.Methods:HPTLC analysis was done to evaluate the presence of phytoconstituents in these plants. Morris water maze and passive avoidance tests were also used to evaluate the effect on memory function. All pretreatment with BME, EAE, TCE, and CEPs was done at 200 mg/kg. The observation was done on the basis of the effect on mean latency time (in seconds) in the Morris water maze test and latency to reach shock free zone (SFZ), mistakes in 15 minutes on the Passive-avoidance test.Results:It was observed that after HPTLC analysis, Bacoside A (Rf value; 0.82) found in BME, flavonoid glycoside (Rf value; 0.31) found in EAE and unknown compounds (Rf value; 0.11, 0.39) observed in TCE. BME, EAE, TCE and their combinations of equal proportion [CEP-1 (BME+EAE), CEP-2 (BME+TCE), CEP-3 (EAE+TCE) and CEP-4 (BME+EAE+TCE)] produced significant effects against scopolamine-induced amnesia in rats.Conclusion:These findings suggested that the content of extracts of these compounds has an antioxidant property in the brain. Also, BME, EAE, and TCE in the CEPs provide a synergistic effect on behavioral assessment.
A Holistic Survey on Disaster and Disruption in Optical Communication Network
Authors: Kumar Dinesh , Sharma Ashutosh , Kumar Rajiv , Sharma Neeru 
Published: April 2020

Background:
This paper is a review of optical fiber communications and also presents
the different types of disasters and different protection schemes to combat these disasters. In the
optical communication network, the failure of a network caused by disasters (e.g. predictable disaster,
unpredictable disaster, Intentional attacks) leads to the failure of several optical communication
channels and huge data loss. Most of the previous works on optical network survivability assume
that the failures are going to occur in future, and the network is made survivable to ensure connectivity
in events of failures. With the advancements in technology, the predictions of tornados, cyclone
and landslides are becoming more accurate by using some early warning systems.

Objective:
Significant improvement in optical communication network survivability can be
achieved using our proposed protection scheme in the event of a major earthquake, cloud burst and
landslides.

Conclusion:
The review concludes that a large amount of data could be lost even if there is a very
short disruption in the optical backbone network.
OS BENEFÍCIOS E DIFICULDADES NA GESTÃO DA QUALIDADE DE VIDA NO TRABALHO
Authors: CARRERA Luiz Carlos Marque , GOMES Roberto Kern , CLAUMANN Patrícia Del Castanhel , ZANARDI Eduardo 
Published: April 2020

RESUMO Vive-se atualmente a Era do Conhecimento, em que as organizações necessitam investir de maneira sistemática em seus recursos humanos, essencialmente pelo fato de que são as pessoas quem podem fazer a diferença nas organizações. Neste contexto, a Qualidade de Vida no Trabalho (QVT) adquire cada vez mais protagonismo no seio destas organizações visando conciliar expectativas pessoais aos objetivos e metas organizacionais. Neste contexto, este trabalho faz uma abordagem ampla sobre o atual estado de conhecimento da QVT, começando pela síntese de suas conceituações e do relato de seu desenvolvimento histórico, passando pela análise crítica de seus principais modelos, com ênfase no modelo de QVT de Walton, um dos mais difundidos no desenvolvimento dos Programas de Qualidade de Vida nas organizações. Assim, através de uma pesquisa bibliográfica, pretende-se identificar quais são estas principais dificuldades e quais os benefícios de se implantar um programa de qualidade de vida no trabalho. Finalmente, conclui-se que o sucesso destes programas se deve principalmente à incorporação de seus principais elementos à cultura organizacional da empresa. Palavras-Chave: Comportamento Organizacional. Qualidade de Vida no Trabalho. Satisfação no Trabalho. ABSTRACT We are now living the Knowledge Age, where organizations need to invest systematically in its human resources, first of all becouse it is people who can make difference in organizations. In this context, Quality of Work Life (QWL) acquires increasing function within these organizations aimed at reconciling personal expectations to organizational goals and objectives. Thus, this paper makes a comprehensive approach on the current state of knowledge of QWT, beginning with the synthesis of its concepts and shows its historical development, through critical analysis of its key models, with emphasis on the QWT model of Walton, one of the most widespread in the development of the Quality of Life Programs in organizations. Thus, through a literature search, we intend to identify what these major difficulties and the benefits of deploying a quality program of work life. Finally, it is concluded that the success of these programs is mainly due to the incorporation of its key elements to the organizational culture of the company. Keywords: Organizational Behavior. Quality of Work Life. Job Satisfaction.
AS QUOTAS PREFERENCIAIS NA SOCIEDADE LIMITADA. CONSIDERAÇÕES SOBRE A PARTIR DA MUDANÇA DE ENTENDIMENTO DA INSTRUÇÃO NORMATIVA Nº 38 DO DREI
Authors: Costa Ilton Garcia da , Vellozo Lucaski Fernando Cesar 
Published: April 2020

Considerando como marco as mudanças introduzidas pela Instrução Normativa nº 38 do Departamento de Registro Empresarial e Integração – DREI que passou a permitir expressamente que as sociedades limitadas adotem institutos próprios das sociedades por ações, que até então eram vedados, o presente trabalho tem como objetivo analisar a adequação desse entendimento em face da realidade dos princípios e institutos jurídicos aplicáveis as sociedades limitadas. Ao longo do estudo, pretende-se revisitar alguns tradicionais institutos jurídicos do direito societário, como o capital social, para então analisarmos a possibilidade de as sociedades limitadas instituírem quotas preferenciais, criando vantagens e restrições, bem como os limites e adequação aos preceitos do Código Civil, a partir da nova interpretação do DREI e das posições doutrinárias sobre o tema e princípios societários aplicáveis. Palavras-Chave: Quotas preferenciais. Instrução Normativa nº 38 do Departamento de Registro Empresarial e Integração – DREI. Sociedade limitada. Sociedade por ações.
CONTRIBUIÇÃO SINDICAL E SUA NATUREZA JURÍDICA
Authors: MACHADO Telma Regina 
Published: April 2020

RESUMOO presente trabalho tem por escopo apresentar o instituto da contribuição sindical, outrora conhecida por “imposto sindical”. Verificar-se-á que especificamente sendo esta figura uma espécie do gênero tributo, foi denominada erroneamente com a nomenclatura “imposto”, mesmo nunca tendo sido enquadrada nos requisitos para sua criação. Será demonstrado os conceitos e definições de cada espécie tributária existente no ordenamento jurídico brasileiro, apontando qual o fato gerador determinante para sua existência. Ainda serão apresentados o fato histórico e a legislação embrionária da contribuição sindical, mesmo com a errônea denominação de “imposto sindical”. Ver-se-á como e porque ela surgiu de forma equivocada e foi tratada por nossos legisladores e doutrinadores. Com a transformação da figura tributária em contribuição sindical, será apresentado para conhecimento gerais, os aspectos dessa espécie e por fim qual a correta caracterização da regra matriz de incidência tributária da contribuição sindical e a concretização como figura de natureza jurídica tributária. PALAVRAS-CHAVE: Contribuição Sindical; Tributo; Natureza Jurídica. ABSTRACTThe present work has as scope to present the institute of the union contribution, formerly known as "union tax". It will be verified that specifically being this figure a kind of tribute genre, was erroneously denominated with the nomenclature "tax", never having been framed in the requirements for its creation. It will be demonstrated the concepts and definitions of each tax species existing in the Brazilian legal system, pointing out the determining factor for its existence. The historical fact and the embryonic legislation of the union contribution will also be presented, even with the erroneous denomination of "union tax". We will see how and why it came about in the wrong way and was treated by our legislators and doctrinaires. With the transformation of the tax figure into union contribution, the aspects of this kind will be presented for general knowledge, and finally, the correct characterization of the matrix rule of tax incidence of the union contribution and the concretization as tax legal nature. KEYWORDS: Union Contribution; Tribute; Legal Nature.
OS CONTRATOS COLIGADOS E SUA SISTEMÁTICA INTEPRETATIVA
Authors: BATISTA Neimar , AMORIM Ana Rosa Tenório de 
Published: April 2020

1 A COMPLEXIDADE DAS RELAÇÕES CONTRATUAIS E SUAS DIFICULDADES HERMENÊUTICASAs relações contratuais, a exemplo do que ocorre com as demais relações da pós-modernidade, tornam-se cada vez mais complexas e cheias de nuances. Os instrumentos contratuais de há muito deixaram de representar contratos típicos, com estipulações puras, para apresentarem um feixe de negócios jurídicos, estipulados num único ou em vários instrumentos contratuais. Assim, as simples relações de consumo passam a apresentar não apenas um contrato de compra, mas também operações financeiras atreladas, com estipulações para cessões de crédito. Nas relações entre as empresas, sobretudo em empreendimentos de grande escala, instrumentos financeiros sofisticados, usados em casos de Project finance, exigem a constituição de instrumentos vários, tais como o de financiamento propriamente dito, as garantias oferecidas, os contratos de seguro atrelados, todos tendo como norte o elemento econômico a conferir unidade aos negócios celebrados. [...]
O NOVO PERFIL DA ADVOCACIA EMPRESARIAL: COMO A TECNOLOGIA DE BLOCKCHAIN IRÁ COMPLEMENTAR DE MANEIRA POSITIVA O COMPLIANCE
Authors: DARIN Luiz Eduardo da Cunha , ASSUMPÇÃO Mariana Baptista de 
Published: April 2020

RESUMO No presente trabalho, através de uma metodologia bibliográfica investigativa, buscase apresentar como o novo perfil da advocacia contemporânea no Brasil, tem se moldado. É utilizado para isso, o marco teórico da aprovação da "Lei Anticorrupção" nesta "nova fase" do Direito brasileiro e como seus desdobramentos se compuseram para termos o que hoje chamamos de Instituto de Compliance. Em um segundo momento, é apresentada uma nova teoria, denominada de Blockchain, que através das considerações feitas durante o trabalho, é capaz de superar as expectativas que o Instituto de Compliance possui, eliminando o Fator Humano do instituto e chegando em uma segurança jurídica de 100%. Conclui-se então que o novo papel da advocacia no Brasil, tem feito um bom trabalho se apropriando de institutos internacionalmente famosos por seus resultados positivos, porém ainda é embrionário, necessitando de mais estudo e interesse. Desta forma teremos um advogado não tão conceitualmente raso, o que possibilitará um referencial positivo ao Profissional de Advocacia Brasileiro e uma maior segurança jurídica na realização de negócios. PALAVRAS-CHAVE: Blockchain; Compliance; Direito Empresarial; Advocacia; Tecnologia. ABSTRACTThe present work, comes through a bibliographic and research methode to present the new contour of brazilian advocacy and how it has been shaped at nowadays cenario. It’s our departure point the theoretical landmark of the “Anti- corruption law” at this new fase of brazilian law as the unfoldings that lead us to the Compliance Institute. Secondly it’s portrayed the new Blockchain theory, and the considerations made include the expectation of overcoming the Compliance Methode as the human factor is eliminated and reaches for the 100% juridical security. Thus, one may conclude that the new rol of advocacy in Brazil has duly appropriated international institutes although at a start-up phase, needing further development and study. This way we belive that it will lead the laywers to a new perspective and enhancement, creating a new referencial and further juridical security at business. KEYWORDS: Blockchain; Compliance; Business Law, Advocacy, Tecnology.