bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development of high-lycopene tomato hybrids using conventional breeding techniques and molecular markers
Authors: Ignatova S. I. , Babak O. G. , Bagirova S. F. 
Published: October 2020

Relevance. High lycopene fruit content has been regarded as a very important genetic trait in tomato breeding. Use lycopene molecular markers in combination with conventional breeding techniques allowed us to create hybrids with high lycopene accumulation, excellent organoleptic qualities, high yield production and resistance to pathogens, and to effectively optimize our breeding programmes for commercial greehouses production.Material and Methods. In this study tomato samples including selected lines and hybrids with various allelic combinations of genes determining carotene accumulation, and other genetic traits, such as disease resistance and yield production were tested. Introgression of spontaneous and induced mutations was used to increase carotenoid levels (og and hp) and improve fruit technological qualities (nor, alc, rin). The research material was tomato collection, mutants, breeding lines and hybrids listed in the State Register Russian Federation tomato hybrids of breeding SS Agrofirm "Ilyinichna" VNIIO branch of the All-Russian Scientific Research Institute of Vegetable Growing – Branch of the FSBSI Federal Scientific Vegetable Center. DNA typing of fruit quality genes was performed at the Institute of Genetics and Cytology of the National Academy of Sciences of Belarus.Results. New domestic hybrids for industrial greenhouses, which characterised by improved organoleptic qualities and technological traits were developed with the help of phasedcross-breeding that allowed to combine the genes nor, rin, alc, leading to an extension of the shelf life with the genes B, og, hp1, etc., contributing to an increase in carotenoid content in fruits. It was established that for targeted selection and hybridization, despite the negative influence of the nor, rin, alc genes it is possible to raise the level of carotenoids to average values. Correlation between lycopene concentration in fruits and high temperature and level of insolation was confirmed. It was shown that pink-fruited forms contain significantly more lycopenethanred-fruitedones. Different all eliccombinations of structural genes involved in carotenoids biosynthesis and regulatory genes that provided maximal accumulation of lycopene in hybrid swithred and pink fruits were revealed. Hybrids with the combination of high concentrations of sugar (° Brix), dry matter and maximal lycopene values, combined defining excellent taste were selected: Prekrasnaiya lady, Olya, Quadrille, Victoria. New F1 hybrids one for industrial greenhouses: G950, G956, G960, Magistral and pink fruited G12897, surpassed the Dutch standard in productivity up to 21%, and in tastes/organoleptic qualities for 1-1.8 points.
General combinative ability of parental lines of watermelon in terms of yield and dry matter content
Authors: Varivoda E. A. , Baybakova N. G. , Varivoda G. V. 
Published: October 2020

Relevance. The creation of heterotic hybrids of watermelon is one of the modern breeding trends for solving the problem of providing the population of the Russian Federation with melon production. The process of creating heterosis hybrids can be accelerated by the assessment of the total combination ability (ОCS) of parental lines.Materials and methods. The studies were carried out in the zone of the Volgograd Trans-Volga region at the Bykovskaya melon selection experimental station, in dry conditions in 2018 and 2019. The object of research was 9 paternal lines and 3 maternal lines of watermelon. The assessment of the general combining ability was carried out according to two indicators: yield and dry matter content.Results. In the course of the research, the obtained F1 watermelon hybrids were assessed for a set of economic characteristics in comparison with the standards: Zenit, Sinchevsky and Barkhan. Based on the results of the work, F1 hybrids were identified according to the length of the growing season, yield, and dry matter content. By selecting valuable genetic sources with a high GCS, it is possible to successfully conduct breeding to improve the economically valuable indicators of F1 watermelon hybrids. According to the results of studies on yield, both in 2018 and in 2019, heterotic hybrids were distinguished, obtained from crossing with the paternal lines Favorit, Stimul, Ikar, the average effect over two years ranged from 3.5-10.6 t/ha. Among the studied maternal lines, the line Chms-4 has a positive effect, the average effect was 1.2 t/ha. In terms of dry matter content, a positive effect was found in the paternal lines Stimul – 1.8%, Medunok – 1.6%, Favorit – 0.8%, and in the maternal line Chms – 41.8%Conclusions. Based on the results of the analysis of the combining ability in terms of yield and dry matter content, we identified two promising paternal lines for creating heterotic watermelon hybrids: Favorite, Stimul. Also, one maternal line Chms-4 was distinguished. When using these lines in heterotic breeding of watermelon, in most crossing combinations, heterosis will be manifested in one or two characters.
Rooting technique of double haploids obtained in culture of microspore <i>in vitro</i> for European radish
Authors: Kozar E. V. , Kozar E. G. , Soldatenko A. V. , Domblides E. A. 
Published: October 2020

Relevance. Doubled haploids (DH-plants) are excellent material for genetic research and breeding due to their complete homozygosity. The genus Raphanus from the Brassicaceae family is the toughest to produce doubled haploid plants through isolated microspore culture in vitro (IMC). The study of the causes of disturbed root formation and the development of elements of this stage of technology will significantly increase the effectiveness of the IMC technology for European radish.Methods. The study included three varieties from the collection of the Federal State Budgetary Scientific Institution Federal Scientific Vegetable Center (FSBSI FSVC): Teplichny Gribovsky, Rozovo-krasniy s belim konchikom and Rhodes. The experiments used a standard protocol for obtaining DH plants using IMC technology in a standard form and with a modification of the rooting stage. The solid MS medium (with agar 7g/L): MS without hormones, MS medium supplemented with IAA at concentrations of 0.5; 1 and 2 mg / L and liquid MSm medium supplemented with 0.1 mg / L kinetin were used for rooting of regenerated plants. All media were supplemented with 20 g/L sucrose. We used three types of techniques for transplanting plant explants onto a solid hormonefree MS medium: planting micro-shoots with their basal part immersed by 2-3 mm into the medium; planting in a well made in a nutrient medium using tweezers under sterile conditions; and landing on the surface of the medium without embedment.Results. In this work, we studied the features of the stage of rooting of regenerated European radish plants in vitro conditions. The transplant technique has been proven to be important for the successful establishment of radish micro-shoots. Plant explants must be planted strictly on the surface of a solid hormone-free nutrient medium MS, without embedment. The use of tubes with bridges made of filter paper and MSm liquid medium with the addition of 0.1 mg/L kinetin for the induction of root formation also showed high efficiency. For plants prone to the formation of root-like structures (RLS) with secondary tumors (ST), multiple dissection of abnormal formations with successive transplants is necessary. Modification at the rooting stage of micro-shoots growing has increased the percentage of successfully adapted DH plants in vivo conditions from 0-14% to 95-98%.
Efficiency of <i>Verbascum densiflorum</i> Bertol. bioregulators in stimulation of vegetable growth and development
Authors: Borovskaya A. D. , Mashchenko N. E. , Ivanova R. A. , Gumanyuk A. V. 
Published: October 2020

Relevance. Increasing the resistance of vegetable plants to unfavorable growing conditions with the use of growth regulators that actively affect the seed quality, increase the survival rate of seedlings, stimulate the setting, formation and maturation of the crop is a significant reserve for increasing productivity with the already achieved level of intensification of their production.Material and methods. The effect of verbascosides obtained by exhaustive extraction with 60% aqueous ethyl alcohol from the aboveground part of Verbascum densiflorum Bertol. (Scrophulariaceae) was studied on the germination, growth, development and yield of tomatoes, cucumbers, onions and white cabbage. Before sowing, the seeds were soaked in an aqueous solution of verbascosides for 15-20 minutes, followed by drying to flowability at the rate of 0.5-0.8 liters of solution per 1 kg of seeds.Results. It is shown, that possessing phytoregulatory activity and influencing the metabolic and synthetic reactions of plants, these compounds, applied exogenously, stimulate the seed germination energy and total germination of vegetable crops, provide simultaneous appearance of seedlings and optimal plant density, accelerate the growth dynamics of plants assimilation surface and fruit mass. Reducing the consequences of stresses arising in the process of plant growth and development, they thereby contribute to increasing plant resistance, productivity and improving the commercial quality of the products. The ability of these compounds to improve the production process of vegetable crops, depending on the type and variety of vegetables was established. Their highest stimulating effect was determined on cabbage variants, where the yield exceeded the control by 34.3%. The performed studies and obtained results allow to recommend the pre-sowing seed treatment with a 0.01% solution of biological active substances from Verbascum densiflorum Bertol. as an element in technology of growing vegetable.
The influence of sodium selenite and 6-benzylaminopurine on the production process of red currant
Authors: Prudnikov P. S. , Golyayeva O. D. 
Published: October 2020

Relevance. In modern climatic conditions, the suppression of production process occurs against the background of short-term or long-term exposure to unfavorable environmental factors on agricultural plants, and, therefore, yield decreases. In this regard, there is a need for the use of growth regulators with protective and stimulating effects on many physiological processes.The aim of the work was to study the influence of the element selenium and 6-benzylaminopurine on the production process of red currant.Methods. Foliar treatments with solutions of sodium selenite and 6-benzylaminopurine (6-BAP) were made after flowering plants of the Dana and Asya varieties of the VNIISPK. The currant leaves were taken from annual shoots for determine the content of abscisic acid (ABA), the amount of pigments, and the rate of light reactions of photosynthesis. The parameters of the fruit brush of plants were recorded.Results. It was shown that treatments with sodium selenite and 6-benzylaminopurine contribute to a decrease of 2.1-3.5 times in the leave tissues of the endogenous ABA level, increase the chlorophyll biosynthesis by 27.5-48.0% and accelerate by 19.3-45.8% electron transfer rate on the level of light photosynthesis reactions. Treatment with selenium and 6-BAP of Dana plants increased the average mass of the fruit brush by 19.30% and 24.5%, respectively, Asya varieties by 20.7 and 35.8%. The increase in the average mass of the fruit brush occurred due to the enlargement of the average mass of one berry in it. It was shown that selenium and 6-BAP in the Dana variety increased the weight of the berry by 27.3% and 47.3%, in Asya by 33.3% and 45.8%. The treatment did not have a significant effect on the amount of sugars. Thus, for increase the production process, it is advisable to make foliar treatments with sodium selenite and 6-BAP during the growing season on red currant plants.
Comparative evaluation of several biologically active compounds content in <i>Anthriscus sylvestris</i> (L.) Hoffm. and <i>Anthriscus cerefolium</i> (L.) Hoffm.
Authors: Kharchenko V. A. , Moldovan A. I. , Golubkina N. A. , Gins M. S. , Shafigullin D. R. 
Published: October 2020

Relevance. A rare representative of the Apiaceae family the garden chervil is widely used abroad in nutrition and in folk and traditional medicine.Methods. Evaluation of nutritional potential of Anthriscus cerefolium (L.) Hoffm and Anthriscus sylvestris Hoffm grown in Moscow region is achieved using the parameters of ascorbic acid, polyphenols, flavonoids, total antioxidant activity of ethanolic extracts.Results. Garden chervil (Anthriscus cerefolium) was characterized by lower concentrations of ascorbic acid (1.6-1.8 times), phenolics (2.2-2.5 times), flavonoids (1.8-2.2 times), total antioxidant activity (1-1.54 times) and dry matter content (2.4-4.9 times) than wild chervil (Anthriscus sylvestris) but demonstrated significantly higher levels of total dissolved solids. Significantly higher content of dry matter in leaves and especially stems of wild chervil correlates with low nutritional value of stems despite large total mass of plants compared to garden chervil. Contrary to wild chervil, Anthriscus cerefolium recorded unusually high concentrations of carotene in leaves and significantly lower levels of chlorophyll b. Seeds of garden chervil were also characterized by low antioxidant status contrary to wild chervil and seeds of curly parsley. High yield and significant nutritional value of garden chervil indicate important prospects of its wide-scale cultivation in Russia while Anthriscus sylvestris plants seem to be important in breeding processes.
Comparative evaluation of several Dynamics of accumulation of lactic and acetic acids in the process of directed fermentation of white cabbage of the Parus variety
Authors: Kondratenko V. V. , Posokina N. E. 
Published: October 2020

Relevance and methodology. Today, white cabbage is the most popular type of vegetable raw materials used for fermentation not only in Russia, but also in other countries. To obtain a variety of flavors and shades of flavor, various vegetables (carrots, sweet peppers), fruit (apples, cranberries) and spicy-aromatic additives (cumin, dill seed, etc.) are added to the cabbage during fermentation. During fermentation, the product contains not only the initial components of the raw material (vitamin C, macro-and micronutrients), but also significantly increases the number of lactic acid microorganisms – the main "participants" in the process.One of the main products of the metabolism of lactic acid microorganisms is lactic acid. At a concentration of 0.5%, it begins to inhibit the development of many microorganisms. When reaching a concentration of 1-2% microbiological enzymatic hydrolysis, as a rule, ceases. The accumulation of acetic acid allows us to judge the intensity of the heterofermentative stage of fermentation using strains of lactic acid microorganisms.Results. The L. plantarum + L. casei consortium has shown a synergistic interaction in the accumulation of lactic acid, since the amount of acid produced by the consortium significantly exceeds the amount of acid produced by each strain separately. This point is also noted for the accumulation of acetic acid. In the L. brevis + L. casei consortium, the opposite situation is observed: when using cultures separately, the accumulation of lactic acid occurs to a greater extent than when using two cultures simultaneously. There are no obvious differences between monocultures and the consortium in terms of acetic acid accumulation. In the case of the L. plantarum + L. brevis consortium, we observe a synergistic interaction, with the dominant role in this consortium played by L. plantarum, and the contribution of L. brevis to the accumulation of lactic acid is insignificant. At the same time, the total resulting accumulation of acetic acid is significantly less than the result of each strain separately, which may indicate a negative mutual effect of the participants in the process. Acid degradation of the polysaccharide matrix of cell walls does not occur during the entire fermentation process.
Study of the autonomous process of inversion of sugarose through thermal influence at ultrasonic processing of vegetable and fruit pures
Authors: Patsyuk L. K. , Fedosenko T. V. , Kondratenko V. V. 
Published: October 2020

Relevance. The article presents the results of a study of the increase in the amount of reducing sugars in the inversion of sucrose due only to thermal effects during ultrasonic processing of vegetable and fruit monocomponent purees (with or without added sucrose). The relevance of the study is to identify the quantitative thermal component of a separate process in the total inversion of sucrose at the specified parameters of ultrasonic action on the product.Material and methods. to perform this task, experimental samples of carrot, pumpkin and Apple puree with the addition of sucrose in an amount of 5% (by weight) and without it were previously made, which were then subjected to heat treatment at the same temperature and time exposure (at a temperature of 50 °C for 30 minutes). To study the dependence of the degree of sucrose inversion on the component composition of the processed product, the mass fraction of reducing sugars – fructose and glucosewas determined in the manufactured experimental samples of natural fruit and vegetable purees (without added sucrose) and in mixtures of purees with sucrose (according to a given recipe) before and after heat treatment at the specified parameters, depending on the recipe composition and pH of the medium.Results. The obtained results of the study of the carbohydrate composition before and after thermal exposure show that the amount of reducing sugars in products subjected to heat treatment has significantly increased, compared to the initial content. Thus, in Apple puree with added sucrose, the mass fraction of fructose after heat treatment of the mixture increased by 197 mg per 100 g, i.e. by 3.8%, and glucose – by 192 mg per 100 g, i.e. by 6.7%. The total invert sugar content of the Apple mixture increased by 4.8 %. In the carrot mixture of puree with sugar, the content of total reducing sugars after thermal exposure increased by 5.4%. At the same time, in carrot natural puree, the increase in reducing sugars was only 3.6%, which is 1.5 times lower. In the pumpkin puree mixture with sugar, the total content of reducing sugars increased by 3.9%, and in the natural puree, the amount of invert sugars increased by only 2.7%, which is 1.4 times lower than puree with added sugar.
ANALISANDO A TRANSIÇÃO DA ÁFRICA DO SUL À DEMOCRACIA: neoliberalismo, transformismo e restauração capitalista
Authors: Barbosa Filho Evandro Alves , Vieira Ana Cristina de Souza 
Published: October 2020

Desde 1994 a África do Sul pôs fim à sua estrutura oficial de segregação, baseada na ultra exploração da força de trabalho negra e na total segregação racial: o Apartheid. Embora esse sistema tenha acabado e o país seja governado pelo antigo movimento de libertação nacional, o African National Congress (ANC), as desigualdades sociais se aprofundaram. O objetivo deste artigo é analisar os processos políticos que condicionaram a transição Sul-africana do Apartheid à democracia. A pesquisa tem natureza qualitativa e foi realizada por meio de revisão bibliográfica da sociologia crítica sulafricana, da análise de documentos oficiais e na análise crítica de discurso. O estudo identificou que a transição à democracia foi tutelada pela mais rica fração da burguesia sul-africana e viabilizada pelo ANC, que aderiu às ideologias neoliberais.Palavras-chave: Apartheid. África do Sul. Transição. Neoliberalismo.ANALYZING SOUTH AFRICA'S TRANSITION TO DEMOCRACY: neoliberalism, transformism and capitalist restorationAbstractSince 1994 South Africa has put an end to its official segregation structure, based on the overexploitation of the black workforce and total racial segregation: The Apartheid. Although this system is over and the country is ruled by the former national liberation movement, the African National Congress (ANC), social inequalities have deepened. This paper aims to analyze the political processes that conditioned the South African transition from Apartheid to democracy. The research has a qualitative approach and It was conducted through a bibliographical review of South African critical sociology, analysis ofofficial documents and critical discourse analysis. The study found that the transition to democracy was led by the wealthiest fraction of the South African bourgeoisie and made possible by the ANC, which adopted the neoliberal ideologies.Keywords: Apartheid. South Africa. Transition. Neoliberalism.
MOBILIDADE HUMANA INTERNACIONAL, POLÍTICAS MIGRATÓRIAS E DIREITOS HUMANOS: avanços e recuos
Authors: Ramos Maria da Conceição Pereira 
Published: October 2020

O contexto atual de crescente e diversificada mobilidade humana a nível global, caracterizado pelo aumento dos fluxos migratórios e do número de refugiados, coloca questões aos níveis económico, social e político e novos desafios às políticas migratórias e à proteção dos direitos humanos. Este artigo pretende refletir sobre o fortalecimento de políticas públicas promotoras da inclusão e do trabalho digno e decente de populações em situação de migração e refúgio, assim como contribuir para uma melhor gestão da mobilidade humana e para a promoção de políticas migratórias que fomentem a coesão social, a defesa dos direitos humanos, a luta contra a discriminação e a desigualdade de oportunidades e a cooperação com os países de origem dos migrantes em favor do desenvolvimento.Palavras-chave: Migrações. Refugiados. Políticas migratórias. Políticas Públicas. Direitos Humanos.INTERNATIONAL HUMAN MOBILITY, MIGRATION POLICIES AND HUMAN RIGHTS: progress and setbacksAbstractThe present scenario of increasing and diversified human mobility at a global level, characterized by increasing migratory flows and refugee populations, raises questions at economic, social and political levels and poses new challenges to migration policies and human rights protection. In this article, we intend to reflect upon the strengthening of public policies that promote inclusion and proper, decent work for migrating and refugee populations, as well as to contribute to a better management of human mobility and the promotion of migration policies that foster social cohesion, the defense of human rights, the fight against discrimination and unequal opportunities, and the cooperation with migrants' countries of origin in favor of development.Keywords: Migration. Refugees. Migration policies. Public policies. Human rights.