bluuebunny/crossref_metadata_2025_split huggingface dataset:
Lipoplex-based targeted gene therapy for the suppression of tumours with VEGFR expression by producing anti-angiogenic molecules
Authors: Ho Shu-Yi , Chen Pin-Rong , Chen Chia-Hung , Tsai Nu-Man , Lin Yu-Hsin , Lin Chen-Si , Chuang Cheng-Hsun , Huang Xiao-Fan , Chan Yi-Lin , Liu Yen-Ku , Chung Chen-Han , Weng Shun-Long , Liao Kuang-Wen 
Published: April 2020

Abstract
Background
The anti-angiogenic fusion protein RBDV-IgG1 Fc (RBDV), which comprises the receptor-binding domain of vascular endothelial growth factor-A (VEGF-A), has shown antitumour effects by reducing angiogenesis in vivo. This study used the cationic lipoplex lipo-PEG-PEI-complex (LPPC) to simultaneously encapsulate both the RBDV targeting protein and the RBDV plasmid (pRBDV) without covalent bonds to assess VEGFR targeting gene therapy in mice with melanoma in vivo.

Results
LPPC protected the therapeutic transgene from degradation by DNase, and the LPPC/RBDV complexes could specifically target VEGFR-positive B16-F10 cells both in vitro and in vivo. With or without RBDV protein-targeting direction, the pRBDV-expressing RBDV proteins were expressed and reached a maximal concentration on the 7th day in the sera after transfection in vivo and significantly elicited growth suppression against B16-F10 melanoma but not IgG1 control proteins. In particular, LPPC/pRBDV/RBDV treatment with the targeting molecules dramatically inhibited B16-F10 tumour growth in vivo to provide better therapeutic efficacy than the treatments with gene therapy with IgG1 protein targeting or administration of a protein drug with RBDV.

Conclusions
The simultaneous combination of the LPPC complex with pRBDV gene therapy and RBDV protein targeting might be a potential tool to conveniently administer targeted gene therapy for cancer therapy.
Identification of novel human adenovirus candidates using the coxsackievirus and adenovirus receptor for cell entry
Authors: Mese Kemal , Bunz Oskar , Schellhorn Sebastian , Volkwein Wolfram , Jung Dominik , Gao Jian , Zhang Wenli , Baiker Armin , Ehrhardt Anja 
Published: April 2020

Abstract
Background
There are over 100 known human adenovirus (HAdV) types, which are able to cause a broad variety of different self-limiting but also lethal diseases especially in immunocompromised patients. Only limited information about the pathogenesis and biology of the majority of these virus types is available. In the present study, we performed a systematic screen for coxsackievirus and adenovirus receptor (CAR)-usage of a large spectrum of HAdV types.

Methods
To study receptor usage we utilized a recombinant HAdV library containing HAdV genomes tagged with a luciferase and GFP encoding transgene. We infected CHO-CAR cells stably expressing the CAR receptor and control cells lacking the CAR receptor with tagged viruses (HAdV3, 14, 16, 50, 10, 24, 27, 37 and 69) and measured luciferase expression levels 26 and for some viruses (AdV10, − 24 and − 27) 52 h post-infection. As positive control, we applied human adenovirus type 5 (HAdV5) known to use the CAR receptor for cell entry. For viruses replication studies on genome level we applied digital PCR.

Results
Infection of CHO-CAR and CHO-K1 cells at various virus particle numbers per cell (vpc) revealed that HAdV10, 24, and 27 showed similar or decreased luciferase expression levels in the presence of CAR. In contrast, HAdV3, 14, 16, 50, 37 and 69 resulted in increased luciferase expression levels in our initial screening experiments. CAR usage of HAdV3, 14, 50, and 69 was not studied before, and therefore we experimentally confirmed CAR usage for these HAdV as novel viruses utilizing CAR as a receptor. To rule out that replication of HAdV in transduced CHO cells is responsible for increased transduction rates we performed replication assays on virus genome level, which revealed that there is no HAdV replication.

Conclusion
In the present study, we screened a HAdV library and identified novel human HAdV using the CAR receptor. To our knowledge, this is the first description of CAR usage for HAdV 3, 14, 50, and 69.
Practice on pressure ulcer prevention among nurses in selected public hospitals, Tigray, Ethiopia
Authors: Berihu Hagos , Wubayehu Tewolde , Teklu Tewolde , Zeru Teklay , Gerensea Hadgu 
Published: April 2020

Abstract
Objectives
Pressure ulcers are localized cellular damages to the skin and underlying tissues caused by pressure, shearing and frictional force. The aim of this study is to assess practices towards pressure ulcer prevention among nurses in the Central Zone of Tigray, Ethiopia, from September 10, 2017 to June 15, 2018. This study has also identified the major barriers that hamper nurses from preventing pressure ulcers. These barriers were heavy workload, inadequate training, and lack of universal guideline and shortage of resource. 17.2% of the participants had a good practice and 82.2% of the respondents had a poor practice of pressure ulcer prevention.

Result
Finding of this study showed that respondents have inadequate knowledge which may have led to their poor practice towards pressure ulcer prevention. Immediate intervention should be done on public hospitals of central Tigray to improve nurses’ practice towards pressure ulcer prevention.
Mindfulness is not associated with dissonant attitudes but enhances the ability to cope with them
Authors: Muschalik Carolin , Crutzen Rik , Elfeddali Iman , de Vries Hein 
Published: April 2020

AbstractBackgroundExplicit and implicit attitudes have been studied extensively, but there is less attention to reducing dissonance between them. This is relevant because this dissonance (IED) results in distress and has inconsistent effects on behavior, e.g. less physical activity but more smoking. Mindfulness decreases dissonance between self-related explicit and implicit constructs. This study investigates if, and which, specific mindfulness subskills are associated with decreased dissonance between explicit and implicit attitudes, and whether mindfulness subskills moderate the relationship between IED and intention/behavior.MethodAt baseline and one and three months thereafter, participants’ (N = 1476) explicit attitudes, implicit attitudes, red meat consumption (RMC), intention to reduce RMC as well as levels of trait mindfulness were assessed.ResultsMindfulness subskills were not associated with decreased IED. IED was associated with lower RMC and a higher intention to reduce RMC. The mindfulness subskill acceptance buffered the effect of IED on intention, seemingly offering a skill to deal with dissonant attitudes, which was unidentified until now.ConclusionThe mindfulness subskill accepting without judgment functions as a way to deal with dissonance. Future research should use this novel finding and investigate whether mindfulness can be used as a buffer in contexts where dissonance results in maladaptive behaviors.
Bone strain index in the prediction of vertebral fragility refracture
Authors: Ulivieri Fabio Massimo , Piodi Luca Petruccio , Rinaudo Luca , Scanagatta Paolo , Cesana Bruno Mario 
Published: April 2020

AbstractDual-energy x-ray absorptiometry (DXA) can provide quantitative (bone mineral density, BMD) and qualitative (trabecular bone score, TBS) indexes of bone status, able to predict fragility fractures in most osteoporotic patients. A new qualitative index of bone strength, based on finite element analysis and named bone strain index (BSI), has been recently developed from lumbar DXA scan. We present the preliminary results about the BSI ability to predict a refracture in patients with fragility fractures. A total of 143 consecutive fractured patients with primary osteoporosis (121 females) performed a spine x-ray examination for the calculation of spine deformity index (SDI) and a DXA densitometry for BMD, TBS, and BSI at basal time and in the follow-up. A refracture was considered as a one-unit increase in SDI. For each unit increase of the investigated indexes, the hazard ratio of refracture, 95% confidence interval, p value, and proportionality test p value were for BSI 1.201, 0.982−1.468, 0.074, and 0.218; for lumbar BMD 0.231, 0.028−1.877, 0.170, and 0.305; and for TBS 0.034, 0.001−2.579, 0.126, and 0.518, respectively. BSI was the index predictive of refracture nearest to statistical significance. If confirmed, it may be used for a better risk assessment of osteoporotic patients.
Can the Pain Attitudes and Beliefs Scales be adapted for use in the context of osteoarthritis with general practitioners and physiotherapists?
Authors: O’Brien Daniel W. , Bassett Sandra , Clair Valerie Wright-St , Siegert Richard J. 
Published: April 2020

Abstract
Background
Conservative, first-line treatments (exercise, education and weight-loss if appropriate) for hip and knee joint osteoarthritis are underused despite the known benefits. Clinicians’ beliefs can affect the advice and education given to patients, in turn, this can influence the uptake of treatment. In New Zealand, most conservative OA management is prescribed by general practitioners (GPs; primary care physicians) and physiotherapists. Few questionnaires have been designed to measure GPs’ and physiotherapists’ osteoarthritis-related health, illness and treatment beliefs. This study aimed to identify if a questionnaire about low back pain beliefs, the Pain Attitudes and Beliefs Scale for Physiotherapists (PABS-PT), can be adapted to assess GP and physiotherapists’ beliefs about osteoarthritis.

Methods
This study used a cross-sectional observational design. Data were collected anonymously from GPs and physiotherapists using an online survey. The survey included a study-specific demographic and occupational characteristics questionnaire and the PABS-PT questionnaire adapted for osteoarthritis. All data were analysed using descriptive statistics, and the PABS-PT data underwent principal factor analysis.

Results
In total, 295 clinicians (87 GPs, 208 physiotherapists) participated in this study. The principal factor analysis identified two factors or subscales (categorised as biomedical and behavioural), with a Cronbach’s alpha of 0.84 and 0.44, respectively.

Conclusions
The biomedical subscale of the PABS-PT appears appropriate for adaptation for use in the context of osteoarthritis, but the low internal consistency of the behavioural subscale suggests this subscale is not currently suitable. Future research should consider the inclusion of additional items to the behavioural subscale to improve internal consistency or look to develop a new, osteoarthritis-specific questionnaire.

Trial registration
This trial was part of the primary author’s PhD, which began in 2012 and therefore this study was not registered.
Life's hard and then you die: the end-of-life priorities of people experiencing homelessness in the UK
Authors: Webb Wendy Ann , Mitchell Theresa , Snelling Paul , Nyatanga Brian 
Published: April 2020

Background: People experiencing homelessness often die young and without adequate support. In the UK, they fail to access palliative care services and their end-of-life priorities remain poorly understood. Aims: To explore the end-of-life concerns, fears, preferences and priorities of a sample of people experiencing homelessness in the UK. Methods: This is an interpretive phenomenology. Data collected through semi-structured, audio-recorded, face-to-face interviews with 21 homeless participants in the UK were analysed iteratively using thematic analysis. Findings have been interpreted through the lens of Merleau-Ponty's philosophy. Results: Eight themes are reported: spiritual concerns; practical concerns; fear of needing care; fear of being forgotten; preference for dying suddenly; preference for being somewhere comfortable where people know me; prioritising autonomy and self-determination; and prioritising authenticity. Conclusion: A strengths-based, trauma-informed, person-centred, collaborative ‘compassionate community’ approach to care is recommended for people experiencing homelessness at end of life.
MXene-Ti3C2Tx for watt-level high-efficiency pulse generation in a 2.8  μm mid-infrared fiber laser
Authors: Wei Chen , Zhou Liqiang , Wang Dongsheng , Chi Hao , Huang Hua , Zhang Han , Liu Yong 
Published: April 2020

We report a watt-level passively

Q

-switched 2.8 μm mid-infrared multi-mode fiber laser by employing multi-layered two-dimensional MXene-

Ti

3

C

2

T

x

as the saturable absorber (SA). The MXene-

Ti

3

C

2

T

x

is fabricated by selectively etching aluminum layers in

Ti

3

AlC

2

. The non-saturable loss, modulation depth, and saturable intensity of the SA at 2866 nm were measured to be 25.0%, 33.2%, and

0.043

GW
/

cm

2

, respectively. The maximum average output power of the

Ti

3

C

2

T

x

Q

-switched fiber laser reached 1.09 W at 28.23% slope efficiency. The pulse repetition rate, shortest pulse width, pulse peak power, and single-pulse energy were 78.12 kHz, 1.04 μs, 13.4 W, and 13.93 μJ, respectively. This is the first demonstration of watt-level pulse generation in a mid-infrared fiber laser using low dimensional materials, to the best of our knowledge. These results indicate that the

Ti

3

C

2

T

x

is a reliable and superior broadband SA for high power mid-infrared pulsed laser generation.
ANALISIS KEPUASAN PELANGGAN ABC LAUNDRY DENGAN MENGGUNAKAN METODE SERVICE QUALITY, IMPORTANCE PERFORMANCE ANALYSIS (IPA) DAN CUSTOMER SATISFACTION INDEX (CSI)
Authors: Hadining Aulia Fashanah 
Published: April 2020

Penelitian ini dilakukan dengan tujuan untuk mengetahui tingkat kepuasan pelanggan terhadap kualitas pelayanan ABC Laundry serta untuk mengetahui apakah ada perbedaan yang signifikan antara persepsi dan harapan pelanggan pada masing-masing dimensi kualitas. Penelitian yang dilakukan merupakan penelitian survei yang bersifat kuantitatif. Responden dalam penelitian ini adalah pelanggan ABC Laundry yang terletak di depan gerbang perumahan Griya Indah. Pengumpulan data yang dilakukan dengan menggunakan kuesioner, pengolahan data yang dilakukan dengan menggunakan metode Servqual, Importance Performance Analysis (IPA) dan Customer Satisfaction Index (CSI). Hasil penelitian menunjukkan bahwa pelanggan sudah merasa sangat puas terhadap pelayanan yang diberikan tetapi belum maksimal. Hal ini terlihat dari hasil pengujian tingkat kepuasan pelanggan dengan menggunakan metode Customer Satisfaction Index (CSI) yaitu sebesar 91%. Pada pengujian dengan metode Importance Performance Analysis (IPA) terdapat 5 atribut yang masuk ke dalam kuadran I yang artinya ada 5 atribut pelayanan yang belum maksimal. Berdasarkan pengujian dengan metode Servqual urutan perbaikan dimulai dari atribut E1 (pihak laundry mampu memberikan penyelesaian atas keluhan dari pelanggan) yang memiliki nilai negatif paling besar. Abstract[Title: Analysis of Customer Satisfaction ABC Laundry by Using Service Quality, Importance Performance Analysis and Customer Satisfaction Index Methode] This research was conducted with the aim to determine the level of customer satisfaction with the quality of ABC Laundry services and to determine whether are significant differences between customer perceptions and expectations in each dimension of quality. The research conducted is quantitative survey research. Respondents in this study were ABC laundry customers located in front of Griya Indah Housing Gate. Data collection is done by using questionnaire, data processing is done by using Servqual, Importance Performance Analysis (IPA) and Customer Satisfaction Index (CSI) methode. The result showed that customers were very satisfied with the service provided but not yet maximized. This can be seen the results of testing the index of customer satisfaction using Customer Satisfaction Index (CSI) methode that is equal to 91%. In testing by using Importance Performance Analyis (IPA) methode, there are 5 attributes that enter into the Quadran I, which means that there are 5 service attributes that have not been maximized. Based on the test by using Servqual methode is the sequence of improvement starts from E1 attribute (the laundry is able to provide settlement of complaints from customers) which has the greatest negative value.Keywords: Customer Satisfaction Index (CSI); Dimension of Service Quality; Importance Performance Analysis (IPA); Servqual
USABILITY TESTING PADA APLIKASI MOBILE JKN BPJS KESEHATAN MENGGUNAKAN DOMAIN SPECIFIC INSPECTION
Authors: Rahmawati Berty Dwi 
Published: April 2020

Pertumbuhan Internet dan teknologi memungkinkan setiap orang mendapatkan setiap informasi secara praktis dan efisien. Badan Penyelenggara Jaminan Sosial Kesehatan atau biasa disebut BPJS Kesehatan merupakan Badan Hukum yang dibentuk untuk menyelenggarakan program jaminan sosial nasional dalam bidang kesehatan untuk seluruh rakyat Indonesia. Perkembangan pesat dalam variabel jumlah peserta terus ditunjukkan oleh BPJS kesehatan setelah 5 tahun beroperasi. Hal ini dapat tercapai karena tingkat kepercayaan masyarakat yang semakin tinggi terhadap layanannya. Indeks kepuasan peserta terus mengalami peningkatan dari 75% menjadi 78,6% pada tahun 2016. Seiring dengan peningkatan indeks kepuasan tersebut, pada tahun 2017 BPJS Kesehatan meluncurkan aplikasi Mobile JKN agar pesertanya dapat secara mudah melakukan pengunduhan data peserta BPJS. Teknologi ini perlu terus dievaluasi dengan metode evaluasi kegunaan untuk mengukur efisiensi, efektivitas, dan menilai kepuasan pengguna. Evaluasi kegunaan ini juga bertujuan untuk mengidentifikasi masalah usabilitas yang muncul dalam pecahan kecil system aplikasi mobile JKN. Metode usabilitas yang digunakan adalah Domain Specific Inspection. Hasil dari penelitian ini adalah nilai efisiensi sebesar >75% pada repetisi kelima. Atribut efektifitas bernilai 3,91 dari skala 5 dan atribut satisfaction bernilai 3,39. Abstract[Title: Usability Testing on BPJS Kesehatan Mobile JKN Application Using Domain Specific Inspection] The growth of Internet and related technology enables everyone to get every information needed in a practical and efficient manner. The Health Social Security Organizing Agency (BPJS Kesehatan) is a legal entity established to organize a national social program in the health sector for all Indonesian. Rapid development in number of participants continued to be shown by health BPJS after 5 years of the operation. This can be achieved due to public trust escalation. Participant satisfaction index continued to increase from 75% to 78.6% in 2016. Along with the increase in the satisfaction index, in 2017 BPJS Kesehatan launched the Mobile JKN application so that participants could easily download BPJS service and personal data. This technology needs to be continually evaluated using Usability Testing methods to measure efficiency, effectiveness, and user satisfaction. This usability evaluation also aims to identify usability problems that arise in a minor fraction of the JKN mobile application system. Method used in this study is Domain Specific Inspection. The results of this study are obtained as follow, efficiency value is higher than 75% after five repetition, effectiveness value achieve 3.91 of scale 5, and user satisfaction value achieve 3.39.Keywords: Application, BPJS Kesehatan; Domain Specific Inspection; Usability Testing
Improvement of Oxidative Stress in Older Women Is Dependent on Resistance Training Volume: Active Aging Longitudinal Study
Authors: Cunha Paolo M. , Ribeiro Alex S. , Padilha Camila , Nunes João Pedro , Schoenfeld Brad J. , Cyrino Letícia T. , Tomeleri Crisieli M. , Nascimento Matheus A. , Antunes Melissa , Fernandes Rodrigo R. , Barbosa Décio S. , Venturini Danielle , Burini Roberto C. , Sardinha Luís B. , Cyrino Edilson S. 
Published: April 2020

Abstract
Cunha, PM, Ribeiro, AS, Padilha, C, Nunes, JP, Schoenfeld, BJ, Cyrino, LT, Tomeleri, CM, Nascimento, MA, Antunes, M, Fernandes, RR, Barbosa, DS, Venturini, D, Burini, RC, Sardinha, LB, and Cyrino, ES. Improvement of oxidative stress in older women is dependent on resistance training volume: Active aging longitudinal study. J Strength Cond Res 36(4): 1141–1146, 2022—The purpose of the present study was to investigate the effects of resistance training (RT) performed with a higher versus lower training volume on oxidative stress (OS) biomarkers in older women. Thirty-eight older women (≥60 years) were randomly assigned to 1 of 2 groups: a group that performed 1 set per exercise (low volume [LV], n = 18) or 3 sets per exercise (high volume [HV], n = 20). The whole-body RT consisted of a 12-week RT program involving 8 exercises performed with sets of 10–15 repetitions maximum, 3 days per week. Advanced oxidation protein products (AOPP), total radical-trapping antioxidant parameter (TRAP), and ferrous oxidation-xylenol orange (FOX) were used as OS biomarkers. The composite Z-score of the percentage changes from pre- to posttraining of OS biomarkers according to groups was calculated. A significant main effect of time (p < 0.05) was found for AOPP (LV = −7.3% vs. HV = −12.2%) and TRAP (LV = +1.5% vs. HV = +15.5%) concentrations, without a statistical difference between the groups (p > 0.05). A significant group vs. time interaction (p < 0.001) was revealed for FOX (LV = +6.4% vs. HV = −8.9%). The overall analysis indicated higher positive changes for HV than LV (composed Z-score: HV = 0.41 ± 1.22 vs. LV = −0.37 ± 1.03; p < 0.05). Our results suggest that a greater volume of RT seems to promote superior improvements on OS biomarkers in older women.
Pacing During 200-m Competitive Masters Swimming
Authors: Breen Derek , Powell Cormac , Anderson Ross 
Published: April 2020

Abstract
Breen, D, Powell, C, and Anderson, R. Pacing during 200-m competitive masters swimming. J Strength Cond Res 34(7): 1903–1910, 2020—Pacing strategies are key to overall performance outcome, particularly in swimming given the large resistive properties of water. However, no studies examining how swimming stroke, gender, age, or performance level affect pacing strategies during 200-m races. This study aimed to examine masters athletes pacing strategies categorized by stroke, gender, age, and performance level. Data were retrieved from World and European masters swimming championships and contained data for 4,272 performances. Performances were coded for stroke, gender, age, and performance classification (PC). Performance classification was based on comparison to the appropriate masters world record. Performances were then normalized, with split times being expressed as a percentage faster or slower than average 50-m split time to determine relative pace. Coefficient of variation (CV) of 50-m time was examined across splits. The main effect for stroke was examined at each split, whereas gender, age, and PC were examined for split-1 pace and CV. An alpha level of 0.05 was set to denote statistical significance. A main effect for stroke was identified at each split (all p < 0.001;

JOURNAL/jscr/04.03/00124278-202007000-00012/inline-formula1/v/2023-08-19T002847Z/r/image-tiff

-split-1 = 0.292;

JOURNAL/jscr/04.03/00124278-202007000-00012/inline-formula2/v/2023-08-19T002847Z/r/image-tiff

-split-2 = 0.040;

JOURNAL/jscr/04.03/00124278-202007000-00012/inline-formula3/v/2023-08-19T002847Z/r/image-tiff

-split-3 = 0.058;

JOURNAL/jscr/04.03/00124278-202007000-00012/inline-formula4/v/2023-08-19T002847Z/r/image-tiff

-split-4 = 0.162). A main effect for PC was identified for split-1 pace and CV within all strokes (all p < 0.001), except for breaststroke (both p > 0.775). Masters athletes exhibit different pacing patterns across strokes, whereas lower ranked athletes also display less even pacing and a faster relative start compared with higher-ranked athletes. Individual analyses of pacing strategies may be necessary.