bluuebunny/crossref_metadata_2025_split huggingface dataset:
KLF13 suppresses the proliferation and growth of colorectal cancer cells through transcriptionally inhibiting HMGCS1-mediated cholesterol biosynthesis
Authors: Yao Weilong , Jiao Yue , Zhou Yanhua , Luo Xiaoya 
Published: June 2020

Abstract
Background
Colorectal cancer (CRC) is the fourth most deadly malignancy throughout the world. Extensive studies have shown that Krüppel-like factors (KLFs) play essential roles in cancer development. However, the function of KLF13 in CRC is unclear.

Methods
The Cancer Genome Atlas database was applied to analyze the expression of KLF13 in CRC and normal tissues. Lentivirus system was used to overexpress and to knock down KLF13. RT-qPCR and Western blot assays were performed to detect mRNA and protein expression. CCK-8, colony formation, cell cycle analysis and EdU staining were used to assess the in vitro function of KLF13 in CRC cells. Xenografter tumor growth was used to evaluate the in vivo effect of KLF13 in CRC. Cholesterol content was measured by indicated kit. Transcription activity was analyzed by luciferase activity measurement. ChIP-qPCR assay was performed to assess the interaction of KLF13 to HMGCS1 promoter.

Results
KLF13 was downregulated in CRC tissues based on the TCGA database and our RT-qPCR and Western blot results. Comparing with normal colorectal cells NCM460, the CRC cells HT-26, HCT116 and SW480 had reduced KLF13 expression. Functional experiments showed that KLF13 knockdown enhanced the proliferation and colony formation in HT-29 and HCT116 cells. Opposite results were observed in KLF13 overexpressed cells. Furthermore, KLF13 overexpression resulted in cell cycle arrest at G0/G1 phase, reduced EdU incorporation and suppressed tumor growth of HCT116 cells in nude mice. Mechanistically, KLF13 transcriptionally inhibited HMGCS1 and the cholesterol biosynthesis. Knockdown of HMGCS1 suppressed cholesterol biosynthesis and the proliferation of CRC cells with silenced KLF13. Furthermore, cholesterol biosynthesis inhibitor significantly retarded the colony growth in both cells.

Conclusions
Our study reveals that KLF13 acts as a tumor suppressor in CRC through negatively regulating HMGCS1-mediated cholesterol biosynthesis.
The effects of park-based interventions on health: a systematic review protocol
Authors: Wallace Deshira D. , Derose Kathryn P. , Han Bing , Cohen Deborah A. 
Published: June 2020

Abstract
Background
Public parks serve as spaces within neighborhoods for encouraging a variety of physical and mental health-related behaviors. Over the past decade, there have been a number of interventions conducted in public parks, often aimed at improving an aspect of mental or physical health. A common type of park-based interventions is aimed at increasing physical activity among adults and children.

Methods
We will conduct a systematic review of peer-reviewed articles on the effects of park-based interventions on physical and mental health outcomes of adults and youth (children and adolescents). An electronic search will be conducted in four electronic databases: Web of Science, PubMed/MEDLINE, Cochrane Library, and Scopus. Manual hand-searching of reference lists from studies identified as relevant by experts and of systematic reviews resulting from the search strategy will be conducted to further identify articles of interest. Inclusion criteria are peer-reviewed, quantitative studies, studies detailing an intervention conducted in a park setting, which was at the person-level or place-level, and studies published in English or Spanish. A three-stage approach will be used to screen title and abstracts and full-text articles against the inclusion and exclusion criteria and, lastly, extract data from eligible studies. Study quality will be assessed by the Cochrane Risk of Bias and the Community Guide’s Guide to Community Preventive Services tools. Extracted data will be summarized narratively and meta-analysis will be conducted, if appropriate.

Discussion
We aim to find relevant studies proving evidence for park-based intervention studies and their effects on health-related outcomes for youth and adults. The evidence obtained from the included studies will help guide future studies on park-based interventions. The study results will be submitted to a peer-reviewed journal for electronic dissemination.

Systematic review registration
Systematic review registration: PROSPERO CRD42018109165.
Changes in soil biochemical properties following replacement of Banj oak forest with Chir pine in Central Himalaya, India
Authors: Manral Vijyeta , Bargali Kiran , Bargali S. S. , Shahi Charu 
Published: June 2020

Abstract
Introduction
In Central Himalaya, anthropogenic activities have led to the widespread replacement of Banj oak (Quercus leucotrichophora) forest by Chir pine (Pinus roxburghii) for decades. This study was conducted to determine how natural Banj oak, Chir pine, and mixed oak-pine forest would differ in soil microbial biomass and soil nutrients. Soil microbial biomass nitrogen (SMBN) and phosphorus (SMBP), soil organic carbon (SOC) total nitrogen (TN), and total phosphorus (TP) in the 0 to 15 cm soil layer were investigated in the Central Himalayan region in the stands of Banj oak, mixed oak-pine, and Chir pine forest.

Results
The SMBN and SMBP were significantly higher in Banj oak and mixed oak-pine forest as compared to Chir pine forest. The ratios of SMBN to TN (SMBN/TN) and SMBP to TP (SMBP/TP) were significantly higher in the Chir pine forest, indicating that in this forest, the proportion of microbial biomass N and P to total soil N and P was higher as compared to Banj oak forest. A similar pattern of variation was found in relation to season across the forests, all with an apparent peak in the rainy season.

Conclusion
These results indicate that low microbial biomass N and P may be one of the reasons to create a nutrient poor site in Chir pine forest. The collection of pine litter by local people also impairs the return of nutrients to the soil and makes it difficult for Banj oak to re-invade areas occupied by Chir pine. This calls for cautions in large-scale conversions of the Banj oak forests to coniferous plantations as a forest management practice on concerns of sustaining soil productivity.
Re-evaluating the relationship between missing heritability and the microbiome
Authors: Douglas Gavin M. , Bielawski Joseph P. , Langille Morgan G. I. 
Published: June 2020

AbstractHuman genome-wide association studies (GWASs) have recurrently estimated lower heritability estimates than familial studies. Many explanations have been suggested to explain these lower estimates, including that a substantial proportion of genetic variation and gene-by-environment interactions are unmeasured in typical GWASs. The human microbiome is potentially related to both of these explanations, but it has been more commonly considered as a source of unmeasured genetic variation. In particular, it has recently been argued that the genetic variation within the human microbiome should be included when estimating trait heritability. We outline issues with this argument, which in its strictest form depends on the holobiont model of human-microbiome interactions. Instead, we argue that the microbiome could be leveraged to help control for environmental variation across a population, although that remains to be determined. We discuss potential approaches that could be explored to determine whether integrating microbiome sequencing data into GWASs is useful.
Review of cellular biophysics and modeling: a primer on the computational biology of excitable cells
Authors: Chaudhry Qasim Ali 
Published: June 2020

Book detailsGreg Conradi SmithWilliam & Mary, Williamsburg, VACellular Biophysics and Modeling: A Primer on the Computational Biology of Excitable CellsCambridge University Press 2019© Greg Conradi Smith 2019DOI: 10.1017/9780511793905ISBN 978-1-107-00536-5 HardbackISBN 978-0-521-18305-5 Paperback
Reviewing assessment of student learning in interdisciplinary STEM education
Authors: Gao Xiaoyi , Li Peishan , Shen Ji , Sun Huifang 
Published: June 2020

AbstractSTEM education has received increasing attention in recent years. However, developing valid and reliable assessment of interdisciplinary learning in STEM has been a challenge. This study is a comprehensive review of assessment of interdisciplinary STEM education during the last two decades. A total of 49 empirical research articles were collected from an initial library of 635 articles focusing on interdisciplinary STEM education. A two-dimensional framework was developed to examine the literature. The first dimension concerns the nature of disciplines being assessed and includes three categories: monodisciplinary, interdiscipline, and transdiscipline. The second dimension concerns the learning objectives and includes four aspects: knowledge, skill, practice, and affective domain. The results show that most assessments focused on assessments of monodisciplinary knowledge, monodisciplinary affective domains, and transdisciplinary affective domains. Although many programs aimed to improve students’ interdisciplinary understanding or skills, their assessments did not align with their aims. Based on the review, future directions are proposed for developing assessments for interdisciplinary STEM educational programs.
Vault-housed extensometers recorded a rapid initial pulse before precursory magma reservoir inflation related to the 2011 eruption of Shinmoe-dake, Japan
Authors: Yamazaki Ken’ichi , Yamashita Yusuke , Komatsu Shintaro 
Published: June 2020

AbstractPrevious studies of the major eruption at Shinmoe-dake volcano, Japan, in January 2011 suggested that gradual injection of magma from a deep source into a shallow reservoir began in December 2009 and led to the major eruption. To investigate the initial phase of this injection event, we examined extensometer data from the Isa Observatory, ~ 18.5 km from the summit of Shinmoe-dake, and discovered a strain change event that spanned about 3 days in December 2009. The size of the strain change is comparable to those observed during each sub-Plinian eruption in 2011. The source of the rapid strain change appears to be deeper than the estimated location of the magma reservoir that directly supplied magma to the 2011 eruption sequence. These observations suggest that rapid injection of magma from the deep magmatic plumbing system in December 2009 triggered the continuous ascent of additional magma from depth, which in turn drove the climactic eruptions in January 2011. Extensometers also recorded two rapid strain change events of the same order of magnitude and with similar characteristics in December 2006 and August 2008; however, noticeable inflation of the edifice was not detected immediately following either event. This suggests that transient injection of magma into a shallow reservoir is not always followed immediately by a gradual recharge process.
Patient-specific instrumentation does not improve tibial component coronal alignment for medial UKA compared to conventional instrumentation
Authors: Kalache Houssam , Müller Jacobus H. , Saffarini Mo , Gancel Evrard 
Published: June 2020

Abstract
Background
Patient-specific instrumentation (PSI) may potentially improve unicompartmental knee arthroplasty (UKA) implant positioning and alignment. The purpose of this study was to compare early radiographic coronal alignment of medial UKA performed using PSI versus conventional instrumentation (CI) for tibial resections.

Methods
A consecutive series of 47 knees (47 patients) received medial UKA, with the tibial resections performed using CI (first 22 knees) or PSI (next 25 knees), while femoral resections were performed with CI in both groups. The target mechanical medial proximal tibial angle (mMPTA) was 87° ± 3°, and the target hip-knee-ankle (HKA) angle was 177° ± 2°. The postoperative mMPTA and HKA were evaluated from postoperative radiographs at a follow-up of 2 months.

Results
Differences in postoperative mMPTA (p = 0.509) and HKA (p = 0.298) between the two groups were not statistically significant. For the mMPTA target, 24% of knees in the PSI group (85.6° ± 2.1°) and 32% of the CI group (85.0° ± 3.6°) were outliers. For the HKA target, 44% of knees in the PSI group (176.3° ± 2.8°) and 18% of the CI group (177.1° ± 2.3°) were outliers. Considering the two criteria simultaneously, 60% of knees in the PSI group and 45% of knees in the CI group were outside the target zone (p = 0.324), whereas 28% of knees in the PSI group and 41% of knees in the CI group were outside the target zone by more than 1° (p = 0.357).

Conclusions
The results of the present study revealed no statistically significant difference in radiographic coronal alignment of UKA performed using PSI versus CI for tibial resections.
School health education program in Pakistan (SHEPP)—a threefold health education feasibility trial in schoolchildren from a lower-middle-income country
Authors: Almas Aysha , Iqbal Romaina , Sabir Sania , Ghani Abdul , Kazmi Khawar 
Published: June 2020

Abstract
Background
The school environment plays an essential role in promoting health education and physical activity for children and adolescence, and they are more likely to adapt it into their adulthood. School health education program has been endorsed and emphasized by the World Health Organization has not been implemented in true spirit in Pakistan yet. We aim to test feasibility of threefold health education program in children and its potential efficacy on physical activity and diet and cardiometabolic risk factors by including BP, BMI, and waist circumference.

Methods
It is a parallel-group feasibility intervention trial. It is being conducted in two schools from lower to middle-income areas, at different locations but having the same school curriculum under the Aga Khan Education Service, Pakistan (AKESP). All children aged 9-11 years enrolled from the schools mentioned above were included. Children with any physical disability were excluded. One school received threefold intervention (focused on children, parents, and teachers) of school health education program in Pakistan (SHEPP) while the other school continued routine activity. Intervention of SHEPP is directed towards educating children, parents, and teachers about healthy behaviors. Children will receive interactive educational sessions and specially designed physical activity sessions. A 3-h health education session focusing on same healthy behaviors as for children will be conducted for both parents and teachers. Primary outcome is feasibility of SHEPP in terms of recruitment, retention, and treatment fidelity. Secondary outcomes are physical activity levels, dietary intake (of fruits, vegetable), and cardiometabolic risk factors (blood pressure, BMI, and waist circumference (WC)). The total number of children recruited were 982 (82.5 %); 505 from school A and 477 from school B and 496 (50.5) were boys.

Conclusion
SHEPP is a unique health education program for children as it focuses on children while involving the parents and teachers in the behavior change process. If found feasible and demonstrating potential efficacy on physical activity, dietary behaviors, and cardiometabolic parameters, we will be able to replicate this on a larger scale in public sector schools also.

Trial registration
NCT03303287
The effect of the tongue in groove technique on the nasolabial angle and nasal tip projection
Authors: Khabir Fatemeh , Sezavar Mehdi , Bohluli Behnam , Mesgarzadeh Vahid , Tavakoli Hamidreza 
Published: June 2020

Abstract
Background
The tongue in groove technique (TIG) is a useful technique for the correction of the nasal tip projection and the nasolabial angle. The purpose of this study was to determine the utility of this technique for nasal tip rotation and projection correction in the Iranian society.

Methods
This is a retrospective clinical trial study of 20 patients undergoing open septo-rhinoplasty using TIG technique from January 2017 to August 2019 at the oral and maxillofacial unit of Bu Ali Hospital and private sector. Preoperative and postoperative profile view photographs were compared to assess the changes in tip projection and rotation.

Results
Fifteen patients (75%) had normal angular size, and 5 of them (25%) were not within the normal range after the surgery. The Fisher exact test showed that this success was statistically significant (P = 0.006). Ten patients (50%) had normal projection size, postoperatively. The Fisher exact test showed that this effect was statistically significant (P < 0.01)

Conclusion
The study demonstrated the benefit of TIG on the correction of nasal tip projection and rotation.
Difficult airway management of a patient with limited range of motion in the temporomandibular joint and cervical extension caused by psoriatic arthritis: a case report
Authors: Maeda Makishi , Chaki Tomohiro , Kawaguchi Ryoichi , Kimijima Tomohiko , Yamakage Michiaki 
Published: June 2020

Abstract
Background
Psoriasis vulgaris, a chronic inflammatory skin disease, rarely causes temporomandibular arthritis. We report a case of difficult airway management of a patient with limited range of motion in the temporomandibular joint and cervical extension caused by psoriatic arthritis.

Case presentation
A 33-year-old man was scheduled to undergo laparoscopic colectomy. On admission, he was diagnosed with psoriatic arthritis. After induction of general anesthesia, we attempted intubation using Pentax Airway Scope® with a thin intlock blade and using a bronchoscope, but it was impossible because of the limited oral space and mandibular elevation. Because of concerns about cannot intubate, cannot ventilate, we antagonized the neuromuscular block and he emerged from general anesthesia. Finally, we succeeded in awake intubation via the nasal cavity using a bronchoscope under spontaneous respiration.

Conclusions
Although psoriasis vulgaris is very rarely associated with temporomandibular arthritis, anesthesiologists should consider that it can cause perioperative difficult airways.
Radiological Society of North America (RSNA) 3D Printing Special Interest Group (SIG) clinical situations for which 3D printing is considered an appropriate representation or extension of data contained in a medical imaging examination: abdominal, hepatobiliary, and gastrointestinal conditions
Authors: Ballard David H. , Wake Nicole , Witowski Jan , Rybicki Frank J. , Sheikh Adnan ,   RSNA Special Interest Group for 3D Printing Abdominal, Hepatobiliary, and Gastrointestinal Conditions Voting Group, Ballard David H. , Sheikh Adnan M. , Weadock William J. , Ryan Justin R. , Matsumoto Jane S. , Souza Carolina , Wake Nicole , Levine Dimitry , Ghodadra Anish , Quigley Edward P. , Christensen Andy , Chepelev Leonid , Althobaithy Waleed , Jeyaraj Satheesh , Krivaniak April , Pietila Todd , Shorti Rami , Santiago Lumarie , Arribas Elsa , Decker Summer , Parthasarathy Jayanthi , Witowski Jan 
Published: June 2020

Abstract
Background
Medical 3D printing has demonstrated value in anatomic models for abdominal, hepatobiliary, and gastrointestinal conditions. A writing group composed of the Radiological Society of North America (RSNA) Special Interest Group on 3D Printing (SIG) provides appropriateness criteria for abdominal, hepatobiliary, and gastrointestinal 3D printing indications.

Methods
A literature search was conducted to identify all relevant articles using 3D printing technology associated with a number of abdominal pathologic processes. Each included study was graded according to published guidelines.

Results
Evidence-based appropriateness guidelines are provided for the following areas: intra-hepatic masses, hilar cholangiocarcinoma, biliary stenosis, biliary stones, gallbladder pathology, pancreatic cancer, pancreatitis, splenic disease, gastric pathology, small bowel pathology, colorectal cancer, perianal fistula, visceral trauma, hernia, abdominal sarcoma, abdominal wall masses, and intra-abdominal fluid collections.

Conclusion
This document provides initial appropriate use criteria for medical 3D printing in abdominal, hepatobiliary, and gastrointestinal conditions.
Sliding mode approach applied to sensorless direct torque control of cage asynchronous motor via multi-level inverter
Authors: El Daoudi Soukaina , Lazrak Loubna , Ait Lafkih Mustapha 
Published: June 2020

AbstractTo improve the robustness and performance of the dynamic response of a cage asynchronous motor, a direct torque control (DTC) based on sliding mode control (SMC) is adopted to replace traditional proportional-integral (PI) and hysteresis comparators. The combination of the proposed strategy with sinusoidal pulse width modulation (SPWM) applied to a three-level neutral point clamped (NPC) inverter brings many advantages such as a reduction in harmonics, and precise and rapid tracking of the references. Simulations are performed for a three-level inverter with SM-DTC, a two-level inverter with SM-DTC and the three-level inverter with PI-DTC-SPWM. The results show that the SM-DTC method achieves better performance in terms of reference tracking, while adoption of the three-level inverter topology can effectively reduce the ripples. Applying the SM-DTC to the three-level inverter presents the best solution for achieving efficient and robust control. In addition, the use of a sliding mode speed estimator eliminates the mechanical sensor and this increases the reliability of the system.
Effect of dietary cassava peel meal supplemented with methionine and multienzyme on hemo-biochemical indices, digestibility, and antioxidants in rabbits
Authors: Oloruntola Olugbenga D. 
Published: June 2020

Abstract
Background
The effects of cassava peel meal (CPM) supplemented with methionine (MET) and multienzyme (ENZ) was studied using 240 5-week-old rabbits in a completely randomized design arranged in 2 × 2 × 2 factorial (2 CPM levels, 0 and 350 g/kg; 2 methionine levels, 5.6 and 8.3 g/kg; and 2 enzyme levels, 0 and 0.5 g/kg).

Results
The CPM inclusion reduced (P < 0.05) leucine, lysine, valine, and threonine digestibility, while the digestibility of leucine, lysine, and valine increased (P < 0.05) by high methionine level. Multienzyme increased leucine, lysine, and valine digestibility. CPM reduced (P < 0.01) red blood cell (RBC) count and the total serum protein. Higher methionine supplementation increased the serum total protein by 9.2%. The CPM inclusion increased bilirubin, alanine aminotransferase (ALT), and aspartate aminotransferase (AST). Methionine and enzyme supplementation increased albumin (ALB) but reduced creatinine (CRE). Superoxide dismutase, glutathione peroxidase, and catalase activity level increased (P < 0.01) with methionine and enzyme supplementation.

Conclusion
Cassava peel meal could be included in growing rabbits’ diets at 350 g/kg level with methionine at 32.53% higher than requirement and 0.5-g/kg multienzyme supplementation.