bluuebunny/crossref_metadata_2025_split huggingface dataset:
The impact of tourists’ perceptions on halal tourism destination: a structural model analysis
Authors: Rahman Mahfuzur , Moghavvemi Sedigheh , Thirumoorthi Thinaranjeney , Rahman Muhammad Khalilur 
Published: February 2020

PurposeThough halal-related services have been discussed in tourism services literature, exploratory study in this area is not enough, particularly in non-Muslim tourists’ perspectives. This study aims to investigate the impact of non-Muslim tourists’ perceptions of halal products or services on their loyalty of intention for halal tourism destination.Design/methodology/approachStructured questionnaires are used for collecting data from non-Muslim tourists. SmartPLS is used in this study for data analysis.FindingsThe results find that banning non-halal services and implementing halal food product services are significantly related to trip experience and trip value. General halal services are positively related to trip value, whereas trip experience is not associated with it. In addition, trip experience and trip value have a significant impact on satisfaction. The results also find that tourists’ loyalty intention is attributed to satisfaction of non-Muslim tourists. `Originality/valueThe study reveals new insight into halal tourism in the perspective of non-Muslim tourists’ halal products or service toward their future travel to halal destination. The contribution of this study is important for tourism operators, managers and marketers of destination tourism with a direct/indirect leadership to improve the tourism destination for non-Muslim tourists’ perceptions.
Ecological strategies begin at germination: Traits, plasticity and survival in the first 4 days of plant life
Authors: Larson Julie E. , Anacker Brian L. , Wanous Sara , Funk Jennifer L. 
Published: February 2020

Abstract

We commonly use trait variation to characterize plant function within and among species and understand how vegetation responds to the environment. Seedling emergence is an especially vulnerable window affecting population and community dynamics, yet trait‐based frameworks often bypass this earliest stage of plant life. Here we assess whether traits vary in ecologically meaningful ways when seedlings are just days old. How do shared evolutionary history and environmental conditions shape trait expression, and can traits explain which seedlings endure drought?

We measured seedling traits in the first 4 days of life for 16 annual plant species under two water treatments, exploring trait trade‐offs, species‐level plasticity and the ability of traits to predict duration of survival under drought.

Nearly half of traits showed the imprint of evolutionary history (i.e. significant phylogenetic signal), often reflecting differences between grasses and forbs, two groups separated by a deep evolutionary split. Water availability altered trait expression in most cases, though species‐level plastic responses also reflected evolutionary history.

On average, new seedlings exhibited substantial trait variation structured as multiple trade‐offs like those found in mature plants. Some species invested in thick roots and shoots, whereas others invested in more efficient tissues. Separately, some invested in tougher roots and others in deeper roots. We also observed trade‐offs related to growth rates (fast or slow) and biomass allocation (above‐ or below‐ground). Drought survival time was correlated most strongly with seed mass, root construction and allocation traits, and phylogeny (grasses vs. forbs).

Synthesis. Our results show that seed and seedling trait variation among annual species is substantial, and that a few attributes could capture major dimensions of ecological strategies during emergence. With seedling survival times ranging twofold among annuals (from 7.5 to 14.5 days), these strategies could mitigate recruitment responses to more frequent or longer dry spells. Multivariate trait and plasticity strategies should be further explored in studies designed to assess trait‐fitness linkages during recruitment.

A free Plain Language Summary can be found within the Supporting Information of this article.
Dynamic Inefficiency and Spatial Spillovers in Dutch Dairy Farming
Authors: Skevas Ioannis , Oude Lansink Alfons 
Published: February 2020

AbstractThis article examines the presence of spatial spillovers in farms’ dynamic technical inefficiency scores using Data Envelopment Analysis and a second‐stage spatial truncated bootstrap regression model. Dynamic inefficiency is measured in terms of variable input contraction and gross investment expansion, while the second‐stage model allows an individual’s dynamic inefficiency to be influenced by both own and neighbours’ characteristics. The empirical application focuses on the panel data of specialised Dutch dairy farms observed over the period 2009–2016 and for which exact geographical coordinates of latitude and longitude are available. The results confirm the existence of spatial spillovers in farmers’ dynamic technical inefficiency levels. Although changes in neighbours’ subsidies do not significantly influence an individual’s inefficiency, an increase in neighbours’ age reduces an individual’s performance, while an increase in neighbours’ levels of intensification improves an individual’s dynamic efficiency.
Nm23‐H1 inhibits lung cancer bone‐specific metastasis by upregulating miR‐660‐5p targeted SMARCA5
Authors: Ai Cheng , Ma Guangzhi , Deng Yunfu , Zheng Qiangqiang , Gen Yingcai , Li Wen , Li Yang , Zu Lingling , Zhou Qinghua 
Published: February 2020

AbstractBackgroundNm23‐H1 gene has been found to be an inhibitor of tumor metastasis in lung cancer. MicroRNAs (miRNAs) play key roles in tumor metastasis through multiple signaling pathways. This study explored whether the nm23‐H1 gene could inhibit invasion and metastasis of lung cancer cells by regulating miRNA‐660‐5p targets.MethodsQuantitative real‐time PCR (qRT‐PCR) and western blots were used to measure the expression of nm23‐H1 and miR‐660‐5p of various human lung cancer cell lines. Cell counting kit‐8 (CCK‐8), wound‐healing and transwell assay were carried out to assess cell proliferation, migration and invasion of each cell line. Xenograft were applied to determine in vivo effects of miR‐660‐5p among nude mice. Luciferase assay and western blot were performed to determine the target gene of miR‐660‐5p.ResultsWe found that high expression of nm23‐H1 correlated with decreased miRNA‐660‐5p expression. Inhibiting miR‐660‐5p suppressed lung cancer cells progression significantly in vitro, whereas overexpression of miR‐660‐5p facilitated tumor growth and bone metastasis in vivo. In addition, as the potential target gene of miR‐660‐5p, SMARCA5 overexpression in vitro suppressed tumor progression and osteolytic metastasis associated RANKL signaling, which is congruent with the effect of nm23‐H1 on the lung cancer cells.ConclusionNm23‐H1 inhibits tumor progression and bone‐specific metastasis of lung cancer by regulating miR‐660‐5p/SMARCA5/RANKL axis, which indicates the related genes may serve as potential targets for the treatment of human lung cancer.Key pointsSignificant findings of the studyHigh expression of nm23‐H1 correlated with decreased miRNA‐660‐5p expression. Further, downregulation of miR‐660‐5p significantly suppressed the tumor progression and bone‐specific metastasis of lung cancer cells.What this study addsThis is the first study to show an inverse association between nm23‐H1 and miR‐660‐5p, and confirm that nm23‐H1 inhibits tumor progression and bone‐specific metastasis of lung cancer by regulating miR‐660‐5p/SMARCA5/RANKL axis.
Characterisation of a novel Emaravirus identified in mosaic‐diseased Eurasian aspen (Populus tremula)
Authors: von Bargen Susanne , Al Kubrusli Rim , Gaskin Thomas , Fürl Stephanie , Hüttner Florian , Blystad Dag‐Ragnar , Karlin David G. , Jalkanen Risto , Büttner Carmen 
Published: February 2020

AbstractSince Emaraviruses have been discovered in 2007 several new species were detected in a range of host plants. Five genome segments of a novel Emaravirus from mosaic‐diseased Eurasian aspen (Populus tremula) have been completely determined. The monocistronic, segmented ssRNA genome of the virus shows a genome organisation typical for Emaraviruses encoding the viral RNA‐dependent RNA polymerase (RdRP, 268.2 kDa) on RNA1 (7.1 kb), a glycoprotein precursor (GPP, 73.5 kDa) on RNA2 (2.3 kb), the viral nucleocapsid protein (N, 35.6 kDa) on RNA3 (1.6 kb), and a putative movement protein (MP, 41.0 kDa) on RNA4 (1.6 kb). The fifth identified genome segment (RNA5, 1.3 kb) encodes a protein of unknown function (P28, 28.1 kDa). We discovered that it is distantly related to proteins encoded by Emaraviruses, such as P4 of European mountain ash ringspot‐associated virus. All proteins from this group contain a central hydrophobic region with a conserved secondary structure and a hydrophobic amino acid stretch, bordered by two highly conserved positions, thus clearly representing a new group of homologues of Emaraviruses. The virus identified in Eurasian aspen is closely associated with observed leaf symptoms, such as mottle, yellow blotching, variegation and chloroses along veins. All five viral RNAs were regularly detectable by RT‐PCR in mosaic‐diseased P. tremula in Norway, Finland and Sweden (Fennoscandia). Observed symptoms and testing of mosaic‐diseased Eurasian aspen by virus‐specific RT‐PCR targeting RNA3 and RNA4 confirmed a wide geographic distribution of the virus in Fennoscandia. We could demonstrate that the mosaic‐disease is graft‐transmissible and confirmed that the virus is the causal agent by detection in symptomatic, graft‐inoculated seedlings used as rootstocks as well as in the virus‐infected scions used for graft‐inoculation. Owing to these characteristics, the virus represents a novel species within the genus Emaravirus and was tentatively denominated aspen mosaic‐associated virus.
OPRM1 Moderates Daily Associations of Naltrexone Adherence With Alcohol Consumption: Preliminary Evidence From a Mobile Health Trial
Authors: Hendershot Christian S. , Dermody Sarah S. , Wardell Jeffrey D. , Zaso Michelle J. , Kennedy James L. , Stoner Susan A. 
Published: February 2020

BackgroundInitial evidence that OPRM1 genotype moderates the clinical response to naltrexone has not been replicated in prospective clinical trials. However, the use of traditional statistical analyses and clinical endpoints might limit sensitivity for studying pharmacogenetic associations, whereas the use of intensive daily assessments and person‐centered analytic methods might increase sensitivity. This study leveraged person‐centered analyses and daily measures of alcohol use, craving, and medication adherence to investigate OPRM1 as a moderator of changes in clinical outcomes during naltrexone treatment.MethodsTreatment‐seeking participants with alcohol use disorder (n = 58; Mage = 38 years; 71% male) provided daily cell phone reports of craving and consumption while taking naltrexone as part of a mobile health trial. Daily medication adherence was measured remotely using electronic pill cap recordings. Multilevel modeling and multilevel structural equation modeling analyses evaluated the hypotheses that OPRM1 genotype would moderate prospective reductions in daily alcohol use and craving, and would also moderate within‐person associations of daily adherence with same‐day craving and consumption.ResultsOPRM1 genotype moderated the association of daily adherence with reduced same‐day consumption (p = 0.007) and craving (p = 0.06), with these associations being stronger for participants with the 118G variant. OPRM1 genotype did not moderate changes in craving and consumption over time.ConclusionsThese findings suggest that high‐density assessments and person‐centered analytic approaches, including modeling within‐person variation in medication adherence, could be advantageous for pharmacogenetic studies.
A large multicentre, randomized, double‐blind, cross‐over study in healthy volunteers to compare pharmacokinetics, pharmacodynamics and safety of a pegfilgrastim biosimilar with its US‐ and EU‐reference biologics
Authors: Bellon Anne , Wang Jessie , Skerjanec Andrej , Velinova Maria , Dickerson Daniel , Sabet Ahad , Ngo Ly , O'Reilly Terry , Tomek Charles , Schussler Steven , Schier‐Mumzhiu Stefanie , Gattu Sreekanth , Koch Sven D. , Schelcher Celine , Dobreva Miryana , Boldea Anca , Nakov Roumen , Otto Gordon P. 
Published: February 2020

AimsRecombinant PEGylated human granulocyte colony‐stimulating factor (pegfilgrastim) is indicated for the reduction of chemotherapy‐induced neutropenia and prevention of febrile neutropenia. Biosimilar pegfilgrastim is expected to reduce the financial burden of this complication of chemotherapy. The aim of this study was to demonstrate biosimilarity between Sandoz biosimilar pegfilgrastim and its US‐ and EU‐approved reference biologics.MethodsPhase I, randomized, double‐blind, single‐dose, 3‐period, 6‐sequence cross‐over, multicentre study to evaluate the pharmacokinetics, pharmacodynamics, safety and immunogenicity of Sandoz biosimilar pegfilgrastim with US‐ and EU‐references in healthy adults.ResultsPharmacokinetic and pharmacodynamic similarity was demonstrated between the 3 biologics, as the 90% confidence interval for all primary pharmacokinetic and pharmacodynamic endpoint comparisons were contained within the predefined similarity margins of 0.80–1.25. Safety, immunogenicity and tolerability were also similar.ConclusionsSandoz biosimilar pegfilgrastim demonstrated pharmacokinetic and pharmacodynamic similarity to both US‐ and EU‐reference biologics. No meaningful differences in safety, local tolerability and immunogenicity were identified.
A 3‐year prospective study of a multidisciplinary early proactive therapeutic drug monitoring programme of infliximab treatments in inflammatory bowel disease
Authors: Sánchez‐Hernández José Germán , Rebollo Noemí , Martin‐Suarez Ana , Calvo M. Victoria , Muñoz Fernando 
Published: February 2020

AimsTherapeutic drug monitoring (TDM) of trough serum infliximab concentrations has been mainly used in case of loss of response in patients with inflammatory bowel disease (IBD). The aim of this study was to evaluate the effectiveness and safety of a multidisciplinary early proactive TDM (mep‐TDM) programme for dose adjustment.MethodsA 3‐year prospective study was conducted based on a sample of 81 patients who started treatment and were subsequently subjected to mep‐TDM with the first control at week 14. Data of a historical control group of 72 patients treated with infliximab and managed with empirical dosing were included. Effectiveness variables were treatment failure, IBD‐related surgery and IBD‐related hospitalization. Safety variables were serious infusion reactions (SIRs) and adverse reactions. Cox regression was used for survival analysis.ResultsIn the mep‐TDM study group, compared to the control group, there was a significant reduction in the risk of treatment failure (hazard ratio [HR]: 0.51; 95% confidence interval [CI]: 0.27–0.92; P = .037), IBD‐related surgery (HR: 0.14; 95% CI: 0.03–0.65; P = .012) and hospitalization (HR: 0.38; 95% CI: 0.17–0.87; P = .022). SIRs were lower in the mep‐TDM group (2.5% vs 10.4%; P < .050); the incidence of adverse reactions was similar (3.7% vs 3.9%; p > .999).ConclusionThis study found that compared to empirical dosing, mep‐TDM is associated with improved efficacy and safety of infliximab therapy, reduced IBD‐related hospitalization and surgery and incidence of SIRs, and increasing long‐term durability of treatment effects.
Designing mate choice experiments
Authors: Dougherty Liam R. 
Published: February 2020

ABSTRACTThe important role that mate choice plays in the lives of animals is matched by the large and active research field dedicated to studying it. Researchers work on a wide range of species and behaviours, and so the experimental approaches used to measure animal mate choice are highly variable. Importantly, these differences are often not purely cosmetic; they can strongly influence the measurement of choice, for example by varying the behaviour of animals during tests, the aspects of choice actually measured, and statistical power. Consideration of these effects are important when comparing results among studies using different types of test, or when using laboratory results to predict animal behaviour in  natural populations. However, these effects have been underappreciated by the mate choice literature to date. I focus on five key experimental considerations that may influence choice: (i) should mating be allowed to occur, or should a proxy behavioural measure of preference be used instead? (ii) Should subjects be given a choice of options? (iii) Should each subject be tested more than once, either with the same or different stimuli? (iv) When given a choice, how many options should the subject choose between? (v) What form should the experimental stimuli take? I discuss the practical advantages and disadvantages of common experimental approaches, and how they may influence the measurement of mate choice in systematic ways. Different approaches often influence the ability of animals to perceive and compare stimuli presented during tests, or the perceived costs and benefits of being choosy. Given that variation in the design of mate choice experiments is likely unavoidable, I emphasise that there is no single ‘correct’ approach to measuring choice across species, although ecological relevance is crucial if the aim is to understand how choice acts in natural populations. I also highlight the need for quantitative estimates of the sizes of potentially important effects, without which we cannot make informed design decisions.
Prognostic value of serum soluble interleukin‐23 receptor and related T‐helper 17 cell cytokines in non‐small cell lung carcinoma
Authors: Liu Dan , Xing Shan , Wang Wan , Huang Xianzhang , Lin Haibiao , Chen Yonghe , Lan Kai , Chen Lin , Luo Fudong , Qin Sheng , Liang Rongliang , Bai Caiying , Xu Jianhua , Liu Wanli 
Published: February 2020

AbstractThe signaling of interleukin (IL)‐23 and its receptor (IL‐23R) play a crucial role in the development of cancers. However, the clinical significance of human serum soluble IL‐23R (sIL‐23R) and its relationship with IL‐23 are still not explored in non‐small cell lung cancer (NSCLC). In our study, sIL‐23R was first identified in the serum of NSCLC patients, but not in healthy controls, by proteomics. The IL‐23R mRNA and protein were upregulated in NSCLC cell lines and tissues tested by quantitative PCR, western blot analysis and immunohistochemistry. The levels of sIL‐23R, IL‐23, and IL‐17 in 195 NSCLC patients’ serum were determined by ELISA, and high levels of sIL‐23R were significantly associated with advanced N stage (P = .039), clinical stage (P = .007), and poor 5‐year survival rate. In vitro, sIL‐23R was shown binding to IL‐23 and the balance could affect patients’ N and T stage, overall survival, and downstream cytokine IL‐17 in a potential antagonistic relationship. Although sIL‐23R, IL‐23, and IL‐17 were all associated with poor prognosis, only the sIL‐23R/IL‐23 ratio (hazard ratio, 1.945; 95% confidence interval, 1.147‐3.299; P = .014) was found to be an independent factor for prognosis. Therefore, we identified fragments of soluble cytokine receptor of IL‐23R with affinity ability to its natural ligand IL‐23 in NSCLC patients’ serum. The balance between the 2 antagonists can work as a potential prognostic serum marker.
Linking children's social care data to information about their care proceedings to understand the use of care proceedings and their effects on parents, children and local authorities.
Authors: Masson Judith , Garside Ludivine , Jenney Sean 
Published: February 2020

AbstractCare proceedings are the most powerful child protection intervention; local authorities make applications for over 20,000 children each year in England and Wales. The Department for Education holds two administrative databases of children who receive services, for children looked after and children in need. These databases do not record the use of care proceedings. Data linkage is a powerful tool for revealing patterns not visible from a single data set; the Outcomes for children before and after care proceedings reform study linked these administrative records with a research database of care proceedings to examine children's care and service journeys associated with care proceedings. The researchers also devised a simplified method for use by local authorities with their own records and tested this with North Yorkshire County Council. Analysis of the linked data reveals how care proceedings are used and the impact of care proceedings on care demand. It provides the basis for analysis of cohorts of children subject to proceedings and feedback to social workers, children's guardians, and the judges and lawyers on their decisions and children's outcomes.
The role of the cancer testis antigen PRAME in tumorigenesis and immunotherapy in human cancer
Authors: Xu Yichi , Zou Ruanmin , Wang Jing , Wang Zhi‐wei , Zhu Xueqiong 
Published: February 2020

AbstractPreferentially expressed antigen in melanoma (PRAME), which belongs to the cancer/testis antigen (CTA) gene family, plays a pivotal role in multiple cellular processes and immunotherapy response in human cancers. PRAME is highly expressed in different types of cancers and is involved in cell proliferation, apoptosis, differentiation and metastasis as well as the outcomes of patients with cancer. In this review article, we discuss the potential roles and physiological functions of PRAME in various types of cancers. Moreover, this review highlights immunotherapeutic strategies that target PRAME in human malignancies. Therefore, the modulation of PRAME might be useful for the treatment of patients with cancer.
Primary cutaneous osteosarcoma of the scalp in an immunosuppressed individual: A case report and review of the literature
Authors: Hunjan Manrup Kaur , Brockley Julia , Zanetto Ulises , Maheshwari M.B. , Díaz Camilo 
Published: February 2020

AbstractWe report a case of a 76‐year‐old man presenting with a 12‐month history of a solitary lesion on his scalp. The histopathology was consistent with a grade 2/3 osteosarcoma extending to the subcutis. Full‐body imaging excluded any involvement of the underlying bony tissue or solid organ malignancy, thus a diagnosis of primary cutaneous osteosarcoma (PCO) was made. Given the exceedingly rare nature of PCO, we discuss the clinico‐pathological features of this case and those previously reported in the literature.