bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prognostic Factors of Hilar Cholangiocarcinoma Patients After Surgical Resection
Authors: Liu Gangshan , Wu Shuodong , Zhao Jiannan , Li Xuecheng , Fan Ying 
Published: May 2020

Abstract
Background

Hilar cholangiocarcinoma (HC) is one of the most common malignancies in China with poor prognosis and its incidence rate is increasing. Surgical resection is the most possible curative treatment option. However, comprehensive knowledge of prognostic markers is less or not accurate. So, the aim of this study was to analyze the prognostic factors in the surgical resection of HC patients.
Methods

A retrospective analysis of 85 cases of HC patients attending our hospital between January 2014 and December 2018 were included in the study. The patients diagnosed as HC and treated with radical surgery were included.Excluded the patients undergoing non-radical surgery, with tumors in other organs, with perioperative death,with death not caused by this disease and with lost follow-up.The clinical data were collected from the Hospital Information System.The patients was follow-up history at July 2019.The end point was that the patient died of recurrence of HC. Cox proportional hazards model analysis was performed to identify indexes of prognosis. All indicators were analyzed by univariate and multivariate analysis.
Results

The significantly related prognostic factors are imaging staging,blood loss during surgery, lymphatic metastasis, tumor size, Tumor Node Metastasis(TNM) stage, surgical margin and level of carbohydrate antigen 19–9(CA19-9) in blood. Among them, TNM stage, surgical margin and level of CA19-9 in blood are independent prognostic factors.
Conclusions

Good prognosis in HC patients is indicated by early stages of TNM staging, no resection margin invaded and low level of CA19-9.
Stem cell application in rotator cuff repair: Interposition stem cell sheet versus overlaid stem cell sheet
Authors: Choi Jae hee , Kang Michael Seungcheol , Shin Myung Jin , Kim Dong Min , Lee Yu Na , Shim In Kyoung , Koh Kyoung Hwan 
Published: May 2020

Abstract
Background

Stem cells are an effective method of biologic healing and can be used to enhance the natural enthesis of the tendon-to-bone junction in rotator cuff repair. The purpose of this study was to investigate if the application of engineered stem cell sheets using adipose-derived cells (ADSCs) was effective in regeneration of natural enthesis and if there was a difference in the result of repair depending on the applied location
Methods

A chronic rotator cuff tear model was induced for 2 weeks, and cell sheets made using ADSCs isolated from rats were transplanted into the tendon-to-bone junction during surgical repair. Depending on the transplant location of the cell sheet, the difference in rotator cuff healing level between the overlaid group and the interposition group was compared to the surgical repair only group. The samples were obtained based on the tendon-to-bone junction and analysis of gross morphology, histology staining, and biomechanical analysis were performed.
Results

The differentiation potentials of ADSCs as stem cells were confirmed, as was the potential for tenogenic differentiation by growth factors. ADSCs were prepared as a sheet form to maintain the shape at the target site and to be easily attached. GFP-expressing ADSCs were proliferated in vivo and observed at the transplantation site. The overall healing level was better in the cell sheet transplanted group than in the control group that surgical repair only. Additionally, differences in healing level were shown depending on the cell sheet location by morphological, histological, and biomechanical perspectives. Histological results showed that the interposition transplantation group (1.75 ± 0.43, P = 0.004) showed better fibrocartilage formation and collagen orientation at the junction than the overlaid transplantation group (0.86 ± 0.83).
Conclusion

In the chronic rotator cuff repair model, the engineered stem cell sheets enhanced the regeneration of the tendon-to-bone junction. This regeneration was more effective when the stem cell sheet was interpositioned at the tendon-to-bone interface.
Trial registration:
Not applicable
The clinical significance of left inferior phrenic vein in retroperitoneal laparoscopic adrenalectomy
Authors: Wang Fang-Ming , Zhang Gui-Ming , Liu Yong 
Published: May 2020

Abstract
Background

The clinical significance of left inferior phrenic vein (IPV) during left retroperitoneal laparoscopic adrenalectomy (RLA) for adrenal tumors is unclear. We aimed to investigate the surgical feasibility and value of IPV as a landmark during RLA.
Methods

Between September 2016 and November 2018, 92 consecutive left RLAs were performed by the same surgeon. All the operations were performed through the approach of three relative avascular planes༎Then we searched for IPV between superior margin of renal artery and anterior aspect of psoas major muscle. The left IPV was used as an anatomical landmark to identify and ligate the central adrenal vein (CAV).
Results

All 92 operations were performed successfully with one conversion to open surgery. We identified the CAV following left IPV in all cases. The left IPV drained into the CAV in 100% of cases, and variant venous anatomy (2 CAVs) was encountered in 2 pheochromocytoma cases (2.17%). Peritoneum perforation occurred in one case in operation (1.09%). Postoperative complications (fever) occurred in 3 patients (3.26%). The mean operative time was 75.82 ± 21.55 minutes༎The average postoperative hospital stay was 3.57 ± 0.76 days༎Pathological examination revealed: cortical adenoma in 72 cases (78.26%), adrenal cyst in 11 cases (11.96%), pheochromocytoma in 6 cases (6.52%), cortical hyperplasia in 1 case (1.09%), medullary hyperplasia in 1 case (1.09%), and hemangioma in 1 case (1.09%).
Conclusions

Using left IPV as a landmark to search for and handle CAV has important clinical significance in left RLA.
Reliability assessment of cutting tools based on zero-failure data
Authors: Yang Bin , Guo Kai , Feng Bin , Zhou Chang'an , Sun Chao , Sun Jie 
Published: May 2020

Abstract
Titanium alloys are widely utilized in aeronautical monolithic components due to their excellent mechanical properties. However, the low machinability of titanium alloys results in an uncertain lifetime of cutting tools. Reliability assessment on cutting tools has become more and more significant to the effectiveness and stability of machining systems. Considering that cutting tools are often replaced well before the end of their useful lifetime to avoid failures and thus few tools failure data can be obtained during the machining process of titanium alloys, a reliability assessment model based on zero-failure data is developed to evaluate the reliability of cutting tools. A Weibull distribution model is chosen to describe the life distribution of cutting tools for reliability evaluation. Matching distribution curve method and weighted least squares method are used to estimate the distribution parameters. A novel approach using hierarchical Bayesian method for estimating the prior distribution of failure probability with zero-failure data is proposed by combining the characteristics of Weibull distribution and Incomplete Beta distribution. Reliability analysis for cutting tools with zero-failure data is performed using the proposed method. By comparing with the experience of experts and adopting stochastic simulation, the results of point estimation show plausibility of the proposed approach.
Comparison of topical breast milk versus dry cord care on umbilical cord separation, complications and cord colonization
Authors: Armanian Amir-Mohammad , Barekatain Behzad , Salehimehr Nima , Sharifian Atefeh , Shirani Zahra , Javid Asma 
Published: May 2020

Abstract
Introduction:

Neonatal death due to infections is still a great concern worldwide. Umbilical cord related infections play significant role in the neonatal mortality specially in very low birth weight infants, mother chorioamnionitis and premature rupture of membranes. It is associated with umbilical colonization, delayed separation and poor hygiene. In the current study, effect of topical breast milk usage with dry cord care on separation time, complications and colonization were compared.
Methods

This is a randomized clinical-trial on 150 very low birth weight neonates in 2016-18. Patients' were divided in two groups; Group-M (N = 73) (3–4 drops of maternal breast milk were dropped on the umbilical cord stump within eight hours of birth and went on every eight hours until 48-hours after cord separation) and Group-D (N = 77) (the umbilical cord was drought gradually in open air). Umbilical cord culturing was performed from cord stump within 2–4 hours after delivery, three days after birth and at the day of separation. Cords were assessed considering omphalitis, bad smell, exudates formation and granulation tissue.
Results

Neonates of two groups were not statistically different regarding separation time (P = 0.09), incidence of omphalitis (P = 0.16), bad odor (P > 0.99), exudative secretions (P = 0.97), granulation tissue (P = 0.96) and sepsis (P = 0.13). In addition, hospitalization duration (P = 0.71), time to reach full enteral feeding (P = 0.31) and death (P > 0.99) were not statistically different. Three times cultures derived from two groups presented no significant difference (P > 0.05).
Conclusion

Based on findings of the current study, neither topical breast milk nor dry cord care was superior considering separation, associated complications and colonization. As neonatal death is still a serious concern worldwide and lacking of unanimous approach for prevention of cord related complications, further studies are strongly recommended.
Overexpressed lncRNA AC068039.4 contributes to proliferation and cell cycle progression of pulmonary artery smooth muscle cells via sponging miR-26a-5p/TRPC6 in hypoxic pulmonary arterial hypertension
Authors: Qin Yuhan , Zhu Boqian , Li Linqing , Yan Gaoliang , Wang Dong , Qiao Yong , Liu Bo , Luo Erfei , Hou Jiantong , Tang Chengchun 
Published: May 2020

Abstract
Background: Hypoxic pulmonary hypertension (HPH) is a devastating and incurable disease characterized by pulmonary vascular remodeling, resulting to right heart failure and even death. Accumulated evidence has confirmed long coding RNAs (lncRNAs) are involved in hypoxia induced pulmonary vascular remodeling in HPH. The exact mechanism of lncRNA in hypoxic pulmonary hypertension remains unclear. Methods：Microarray analysis was applied to investigate the profiles of lncRNA expression in pulmonary artery smooth muscle cells (PASMCs) cultured under hypoxia and normoxia condition. qRT-PCR was performed for the expression of lncRNAs, miRNA and mRNAs, western blot analysis was employed for detection the expression of proteins. CCK-8 and transwell chamber assay were applied for assessment of PASMC proliferation and migration, respectively. Besides, flow cytometry was performed for assessments of cell cycle progression. The binding between AC068039.4 and miR-26a-5p, miR-26a-5p and TRPC6 3’UTR were detected by dual luciferase reporter assay.Results：A total of 1211 lncRNAs (698 up-regulated and 513 down-regulated) were differently expressed in hypoxia induced PASMCs. Consistent with microarray analysis, quantitative PCR verified that AC068039.4 was obviously up-regulated in hypoxia induced PASMCs. Knocking down AC068039.4 alleviated proliferation and migration of PASMCs and regulated cell cycle progression through inhibiting cells entering the G0/G1 cell cycle phase. Further experiment indicated AC068039.4 promoted hypoxic PASMCs proliferation via sponging miR-26-5p. In addition, transient receptor potential canonical 6 (TRPC6) was confirmed to be a target gene of miR-26a-5p. Conclusion: In conclusion, downregulation of lncRNA AC068039.4 inhibited pulmonary vascular remodeling through AC068039.4/miR-26a-5p/TRPC6 axis, providing new therapeutic insights for the treatment of HPH.
Functional Compensation Dominates Plant Rhizosphere Microbiota Assembly
Authors: Ren Yi , Xun Weibing , Yan He , Ma Aiyuan , Xiong Wu , Shen Qirong , Zhang Ruifu 
Published: May 2020

Abstract
Background: Rhizospheremicrobiota plays a key role in plant-soil feedbacks, yet the intrinsic principles governing rhizospheremicrobiota assembly remain unclear. To understand the principles, we studied taxonomical and functional characteristics of re-assembled maize rhizospheremicrobiota during transplanting between soils. The composition of rhizospheremicrobiota were measured by High-throughput amplicon-based IlluminaMiSeq sequencing of 16S ribosomal RNA (rRNA) gene V4-region. The rhizosphere functional profiles were assessed using 16S rRNA data through PICRUSt (Phylogenetic Investigation of Communities by Reconstruction of Unobserved State) software. Results: The re-assembled rhizospheremicrobiota was recruited from both soil and endosphere communities. Soil nutrient availability dominatedmicrobiota assembly and functional profile in rhizosphere. The most important rhizospheric functions were stress tolerance, while the related taxa were in low abundance and derived primarily from bulk soil. Nutrient excessive soil marginalized rhizosphere nutrient cycling functions, while nutrient deficient soil sacrificed stress tolerance functions and enhanced compensatory colonization of nutrient cycling related endophytes in rhizosphere. Conclusions: Our results demonstrate soil condition mediates rhizospheremicrobiota assembly to satisfy plant required functions. This assembly principle is helpful for manipulating plant root microbiota to improve agricultural sustainability.
Effectiveness of A 24-h Access Outpatient Clinic for Patients with Chronic Conditions in Hospital Outpatient Follow-Up: A Registry-Based Controlled Cohort Study of Healthcare Utilisation and Mortality
Authors: Schlünsen Anders Damgaard Møller , Christiansen David Høyrup , Fredberg Ulrich , Vedsted Peter 
Published: May 2020

Abstract
Background: In 2015, Regional Hospital Silkeborg in Denmark introduced a 24-h telephone access hospital outpatient clinic (24-h access clinic). The 24-h access clinic allowed patients to call the hospital outpatient clinic in case of an exacerbation of symptoms. The aim of this study was to evaluate the effectiveness of a 24-h access clinic in terms of healthcare utilisation and mortality in patients with chronic conditions.Methods: This study was designed as a registry-based controlled cohort study. The 24-h access clinic was established at Silkeborg Regional Hospital in Central Denmark Region, and the five other regional hospitals served as comparison hospitals. We included patients in hospital outpatient follow-up with chronic obstructive pulmonary disease, congestive heart failure, atrial fibrillation/flutter, inflammatory bowel disease and chronic liver disease. Outcomes were hospital admissions, length of stay (LOS), intensive care unit assistance and outpatient visits, contacts to general practice, municipal home nursing and all-cause mortality. Follow-up was 18 months.Results: The study included 992 24-h access patients and 3,878 usual care patients. For the five conditions combined, the 24-h access patients had fewer all-cause admissions (incidence rate ratio (IRR) = .81, 95% confidence interval (Cl) = .71 to .92), general practice out-of-hours contacts (IRR = .81, CI = .71 to .92) and shorter LOS (IRR = .71, CI = .57 to .88), but the rate of all-cause outpatient visits was higher (IRR = 1.07, CI = .99 to 1.15). General practice daytime contacts were similar between the groups, and there was no significant difference in mortality.Conclusions: A 24-h access clinic reduced acute admissions and LOS compared to usual outpatient care. Planned outpatient visits increased and substituted unplanned acute care, thus enhancing integration of care by allowing for access to the correct level of care and ensuring continuity of care.
Molecular Characterization of Some Equine Vector-Borne Pathogens and Identification of Their Vectors in Egypt
Authors: Abdullah Hend H.A.M. , Aboelsoued Dina , Farag Tarek K , Megeed Kadria N Abdel , Abdel-Shafy Sobhy , Parola Philippe , Raoult Didier , Mediannikov Oleg 
Published: May 2020

Abstract
Background Equine vector borne diseases (EVBD) have been considered emerging and reemerging diseases transmitted by arthropods and most of these diseases have zoonotic concern. This study was designed to screen EVBD in equines and their vectors using molecular analyses and identify vectors by MALDI-TOF and molecular techniques.Methods A total of 335 blood samples were collected from apparently healthy equines (320 from horses and 15 from donkeys) from Cairo and Beni-Suef provinces in Egypt. A total of 166 arthropods (105 sucking flies and 61 ticks) were collected from the same animals. MALDI-TOF and molecular techniques were used to confirm the findings of morphological identification of vector. Quantitative PCR and Standard PCR coupled with sequencing were performed in equines and vectors DNA for screening multiple pathogens.Results MALDI-TOF and molecular techniques confirmed that Hippoposca equina (louse fly), Rhipicephalus annulatus (Rh. annulatus) and Rh. microplus ixodid ticks were found. In vectors, we identified Anaplasma marginale (A. marginale; 1.6%), A. platys-like (1.6%) and a new Ehrlichia sp. (4.9%) in Rh. microplus, while Ehrlichia rustica (E. rustica) was found in Rh. microplus and Rh. annulatus. Likewise, Borrelia theileri was identified in Rh. microplus (3.3%). For H. equina, Anaplasma and Borrelia sp. DNA were detected by qPCR only. In equines, A. marginale (0.6%), A. ovis (0.6%) and Theileria ovis (T. ovis; 0.6%) were found in donkeys. In horses, T. equi (1.2%) and a new Theileria sp. Africa (2.7%) were identified.Conclusions For the first time, we reported here the presence of Rh. microplus as a competent tick for Rh. annulatus in Egypt using MALDI-TOF and molecular identification. To the best of our knowledge, we provided the first detection of different pathogens as A. marginale, A. platys-like, E. rustica, new Ehrlichia sp., B. theileri in Rh. microplus, A. marginale, A. ovis and T. ovis in donkeys and a new Theileria sp. Africa in horses in Egypt.
Effect of gut microbiota modulation on feeding tolerance of enterally-fed critically ill adult patients: a systematic review
Authors: Seifi Najmeh , Esfahani Ali Jafarzadeh , Sedaghat Alireza , Rezvani Reza , Khadem-Rezaiyan Majid , Nematy Mohsen , Safarian Mohammad 
Published: May 2020

Abstract
Purpose The objective of this systematic review was to evaluate the effect of pre-, pro-, and synbiotics on feeding tolerance of enterally-fed critically ill adult patients. Methods Medline, Science Direct, Web of Knowledge, and the Cochrane Central Register of Controlled Trials were searched up to November 2019. English language randomized controlled trials reporting the effect of pre, pro or synbiotics on the feeding tolerance of enterally-fed critically ill adult patients were included. Results Among six studies reporting the energy intake, only two studies showed significantly higher energy intake in the prebiotic-receiving groups. Among four RCTs reporting frequency or time to achieve the target calorie, only one found a significant effect of probiotics to reduce the time to achieve a target dose of calorie. About the prevalence or duration of diarrhea, 7 out of 12 RCTs reported a beneficial effect. All but one study found no beneficial effects for gut microbiota manipulation on clinical endpoints including LOS in hospital and ICU. Conclusion It should be noticed that the heterogeneity in study designs, product format, and ICU patient populations makes it difficult to draw any general conclusion. Overall, it seems that pre, pro or synbiotics have not significant beneficial effect on feeding tolerance and clinical endpoints in critically ill adults, but they may reduce the prevalence or duration of diarrhea.
ZNF280A Promotes Lung Adenocarcinoma Development through Regulating the Expression of EIF3C
Authors: Liu Hongsheng , Qin Yingzhi , Zhou Na , Ma Dongjie , Wang Yingyi 
Published: May 2020

Abstract
Background: Lung cancer is the most commonly diagnosed malignant tumor worldwide. Lung adenocarcinoma (LUAD) is the most common histological subtype in non-small cell lung cancer (NSCLC). The relationship between ZNF280A and LUAD has not been demonstrated and remains unclear. Methods: In this study, it was demonstrated that ZNF280A was upregulated in LUAD tissues compared with the normal tissues. Further investigations indicated that the overexpression/knockdown of ZNF280A could promote/inhibit proliferation, colony formation and migration of LUAD cells, while inhibiting/promoting cell apoptosis. Moreover, knockdown of ZNF280A could also suppress tumorigenicity of LUAD cells in vivo. RNA-sequencing followed by Ingenuity pathway analysis (IPA) was performed for exploring downstream of ZNF280A and identified EIF3C as the potential target. Results: Furthermore, our study revealed that knockdown of EIF3C could inhibit development of LUAD in vitro, and alleviate the ZNF280A overexpression induced promotion of LUAD. Conclusions: In conclusion, our study showed, as the first time, ZNF280A as a tumor promotor for LUAD, whose function was carried out probably through the regulation of EIF3C.
Prevalence of Diabetic Retinopathy and Its Associated Factors Among Diabetic Patients at Debre Markos Referral Hospital, North-west Ethiopia, 2019.
Authors: Tilahun Melkamu , Gobena Teshome , Dereje Diriba , Wolde Mengistu , Yideg Getachew 
Published: May 2020

Abstract
Background

Diabetic retinopathy is a well-known sight–threatening microvascular complication of diabetes mellitus. Currently 93 million people live with diabetic retinopathy worldwide. There are insufficient studies addressing on the prevalence of diabetic retinopathy and underlying risk factors in Ethiopia.
Objective

To assess prevalence of diabetic retinopathy and its associated factors among diabetic patients on follow up at Debre Markos Referral Hospital, North-West Ethiopia, 2019.
Methods

Institutional based cross-sectional study was conducted among 302 participants at Debre Markos Referral Hospital. They were selected through systematic sampling technique and those with mature cataract and critically ill were excluded. The necessary data were extracted from medical records by using pre-tested checklist. Blood pressure, weight, height and visual acuity tests were assessed. Retinal examination was performed through slit lamp biomicroscope and binocular indirect ophthalmoscope. Data were entered to epi-data 3.1 version. The data were exported into Statistical Package for Social Science (SPSS version 20) program for analyses. Binary logistic regression with 95%CI was used for analyses. A variable had p-value < 0.25 in the bivariable logistic regression was entered to multivariate logistic regression. Finally, variables with p-value < 0.05 in the multivariable logistic regression were considered as statistically significant.
Result

There were 302 participants included in this study, out of which 57(18.9%) had diabetic retinopathy. Among DR patients, three-fourth (75.4%) had pre-proliferative type of diabetic retinopathy. Four in ten (37.7%) of diabetic patients had visual acuity problem. Poor glycaemic control (AOR(95% CI: 4.58(1.86,11.31), having more than 10 years diabetic duration (AOR(95% CI: 3.91(1.86,8.23), body mass index > 25 kg/m2(AOR(95% CI: 3.74(1.83,7.66), and hypertension (AOR(95% CI: 3.39(1.64,7.02) were significantly associated factors with diabetic retinopathy.
Conclusion

About one fifth of DM patients had diabetic retinopathy. Diabetic retinopathy was significantly associated with glycaemic control, hypertension, body mass index and duration of illness. Routine assessment and early control of those associated factors may be important to reduce both the prevalence and impact of diabetic retinopathy as evidenced in the current study.