bluuebunny/crossref_metadata_2025_split huggingface dataset:
Treadmilling FtsZ polymers drive the directional movement of sPG-synthesis enzymes via a Brownian ratchet mechanism
Authors: McCausland Joshua W. , Yang Xinxing , Squyres Georgia R. , Lyu Zhixin , Bruce Kevin E. , Lamanna Melissa M. , Söderström Bill , Garner Ethan C. , Winkler Malcolm E. , Xiao Jie , Liu Jian 
Published: November 2019

AbstractFtsZ, a highly conserved bacterial tubulin GTPase homolog, is a central component of the cell division machinery in nearly all walled bacteria. FtsZ polymerizes at the future division site and recruits greater than 30 proteins to assemble into a macromolecular complex termed the divisome. Many of these divisome proteins are involved in septal cell wall peptidoglycan (sPG) synthesis. Recent studies found that FtsZ polymers undergo GTP hydrolysis-coupled treadmilling dynamics along the circumference the division site, driving the processive movement of sPG synthesis enzymes. How FtsZ’s treadmilling drives the directional transport of sPG enzymes and what its precise role is in bacterial cell division are unknown. Combining theoretical modeling and experimental testing, we show that FtsZ’s treadmilling drives the directional movement of sPG-synthesis enzymes via a Brownian ratchet mechanism, where the shrinking end of FtsZ polymers introduces an asymmetry to rectify diffusions of single sPG enzymes into persistent end-tracking movement. Furthermore, we show that the processivity of this directional movement is dependent on the binding potential between FtsZ and the enzyme, and hinges on the balance between the enzyme’s diffusion and FtsZ’s treadmilling speed. This interplay could provide a mechanism to control the level of available enzymes for active sPG synthesis both in time and space, explaining the distinct roles of FtsZ treadmilling in modulating cell wall constriction rate observed in different bacterial species.
Targeting DDR1 and DDR2 overcomes matrix-mediated melanoma cell adaptation to BRAF-targeted therapy
Authors: Berestjuk Ilona , Lecacheur Margaux , Diazzi Serena , Rovera Christopher , Prod’homme Virginie , Mallavialle Aude , Larbret Frédéric , Pisano Sabrina , Audebert Stéphane , Passeron Thierry , Gaggioli Cédric , Girard Christophe A. , Deckert Marcel , Tartare-Deckert Sophie 
Published: November 2019

AbstractResistance to BRAF and MEK inhibitors in BRAFV600Emutant melanomas remains a major obstacle that limits patient benefit. Microenvironment components including the extracellular matrix (ECM) can support tumor cell adaptation and tolerance to targeted therapies, however the underlying mechanisms remain poorly understood. Here, we investigated the process of matrix-mediated drug resistance (MM-DR) in response to BRAF inhibition in melanoma. We demonstrate that physical and structural cues from fibroblast-derived ECM abrogate anti-proliferative responses to BRAF/MEK inhibition. MM-DR is mediated by the drug-induced clustering of DDR1 and DDR2, two tyrosine kinase collagen receptors. Genetic depletion and pharmacological inhibition of DDR1 and DDR2 overcome ECM-mediated resistance to BRAF inhibition. In melanoma xenografts, targeting DDRs by Imatinib enhances BRAF inhibitor efficacy, counteracts drug-induced collagen remodeling and delays tumor relapse. Mechanistically, DDR-mediated MM-DR fosters a targetable pro-survival NIK/IKKα/NF-κB2 pathway. Our study reveals a novel role of collagen-rich matrix and DDRs in tumor cell adaptation and therapy resistance, thus providing important insights into environment-mediated drug resistance and a pre-clinical rationale for targeting DDR1/2 signaling in combination with BRAF-targeted therapy in melanoma.
Regulator of Gene Silencing-Calmodulin associates with mRNA granules and the autophagy protein ATG8
Authors: Craig Conner W. , Lokdarshi Ansul , Roberts Daniel M. 
Published: November 2019

AbstractRegulator-of-gene-silencing calmodulins (rgsCaM) represent a phylogenetic subfamily of calmodulin-like calcium sensors that are targets of viral induced suppression of posttranscriptional gene silencing by secondary siRNAs. The present work shows that a stress (hypoxia) that induces mRNP granule formation also induces the relocalization of rgsCaM to cytosolic granule-like foci that interact with the surface of stress granule and processing body structures. Co-expression of rgsCaM and its binding protein Suppressor of Gene Silencing 3 causes re-localization and integration of rgsCaM into stress granule structures. RgsCaMs contain a conserved topology that consists for four EF hand like domains (three functional and one divergent) that are separated into two calcium binding lobes with an extended amino terminal region. RgsCaM also contains an “ATG8 family interacting motif” (AIM) within its amino-terminal domain that is characteristic of selective autophagy cargo receptors. Co-localization experiments and ratiometric BiFC analyses inNicotiana benthamianasupport the hypothesis that rgsCaM binds directly to ATG8e through this conserved AIM domain, and the two proteins co-localize with mRNP granule markers. Previous reports show that rgsCaM mediates the suppression of gene silencing, at least in part, via turnover of SGS3 via autophagy. A model is proposed for rgsCaM-like proteins as potential mediators of selective autophagy of RNA granules in response to biotic and abiotic stresses.
CD300lf is the primary physiologic receptor of murine norovirus but not human norovirus
Authors: Graziano Vincent R. , Walker Forrest C. , Kennedy Elizabeth A. , Wei Jin , Ettayebi Khalil , Simões Madison S. , Filler Renata B. , Hassan Ebrahim , Hsieh Leon L. , Kolawole Abimbola O. , Wobus Christiane E. , Lindesmith Lisa C. , Baric Ralph S. , Estes Mary K. , Orchard Robert C. , Baldridge Megan T. , Wilen Craig B. 
Published: November 2019

AbstractMurine norovirus (MNoV) is an important model of human norovirus (HNoV) and mucosal virus infection more broadly. Viral receptor utilization is a major determinant of cell tropism, host range, and pathogenesis. Thebona fidereceptor for HNoV is unknown. Recently, we identified CD300lf as a proteinaceous receptor for MNoV. Interestingly, its paralogue CD300ld was also sufficient for MNoV infectionin vitro. Here we explored whether CD300lf is the sole physiologic receptorin vivoand whether HNoV can use a CD300 ortholog as an entry receptor. We report that both CD300ld and CD300lf are sufficient for infection by diverse MNoV strainsin vitro. We further demonstrate that CD300lf is essential for both oral and parenteral MNoV infection and to elicit anti-MNoV humoral responsesin vivo. In mice deficient in STAT1 signaling, CD300lf is required for MNoV-induced lethality. However, after high dose intraperitoneal challenge with MNoV inCd300lf−/−Stat1−/−mice a single amino acid mutation in the MNoV capsid protein emerged. This substitution did not alter receptor utilizationin vitro. Finally, we demonstrate that human CD300lf (huCD300lf) is not essential for HNoV infection, nor does huCD300lf inhibit binding of HNoV virus-like particles to glycans. Thus, we report huCD300lf is not a receptor for HNoV.Author SummaryHuman norovirus is the leading cause of non-bacterial gastroenteritis causing up to 200,000 deaths each year. How human norovirus enters cells is unknown. Because human norovirus is difficult to grow in the laboratory and in small animals, we use mouse or murine norovirus as a model system. We recently discovered that murine norovirus can use the either CD300ld or CD300lf as a receptorin vitro. We also showed that CD300lf deficient mice were resistant to oral challenge with a single virus strain. Here we determined that CD300lf is essential for infection of diverse murine norovirus strains in cell lines and in mice with normal immune systems demonstrating it’s the primary physiologic receptor for diverse murine norovirus strains independent of infection route. However, in immunodeficient mice injected with high dose virus directly into the abdominal cavity, we observed a norovirus mutant that enabled CD300lf-independent infection. Finally, we demonstrated that human CD300lf is not the elusive receptor for human norovirus.
Implementing evaluation for positive organizational changes: IBA-public school Sukkur, Pakistan
Authors: Umrani Waheed Ali , Solangi Rukhman , Ali Memon Mumtaz , Hadeesa Asmaa , Khoso Soonhan 
Published: November 2019

Learning outcomes
Learning outcomes are as follows: Understand performance appraisal process and tools; apply theory X and Theory Y in managing resistance to performance evaluation; identify the causes and symptoms of resistance; identify and apply managing resistance approaches.

Case overview/synopsis
After attaining the height of success in terms of imparting quality education and contributing to the creation of many learned persons of the society, Public school Sukkur was facing the downward trending success for many reasons. After the takeover of management control by Sukkur IBA University, the school was upward trending for quality education, state of the art infrastructure, advanced educational lab, modern teaching methodologies. With such a change, resistance was a must. Both Active and Passive resistance from the stakeholders was impeding the success of newly named IBA-Public School Sukkur. Particularly, the resistance against the implementation of the Performance Appraisal tool and its administration. With the resistance from employees, Chang, Principal IBA Public School Sukkur had to come up a solution for the smooth administration and implementation of Performance Appraisal and manage the resistance from the employees and ensure the continuous improvement through performance appraisal.

Complexity academic level
Case study is applicable for the MBA students.

Supplementary materials
Teaching Notes are available for educators only. Please contact your library to gain login details or email support@emeraldinsight.com to request teaching notes.

Subject code
CSS 7: Management Science.
Bike Wala – A To Zee Distribution
Authors: Shaikh Abdul Rehman , Qazi Asad Ali 
Published: November 2019

Learning outcomes
Learning outcomes are as follows: to understand the influence of external factors in operations planning; to understand the importance of contingency planning in new initiatives; to analyze financial and non-financial gains of the new project; to analyze and visualize the unexpected challenges in post-implementation of project; and to critically analyze the ethical consideration in decision-making.

Case overview/synopsis
After joining A to Zee Distribution, Shaikh had brought up several improvements to distribution operations and had suggested various cost-saving initiatives. He had also developed and implemented the distribution KPIs for the first time in the organization. One of the recent initiatives that he took was the start of a new project named ‘Bike Wala’. This project not only increased the market base for A to Zee but also significantly reduced their operations expenses. However, after around two months of the project, the delivery officers reported several incidents and accidents. These accidents included several injuries due to unbalancing bikes and excess load/weight in delivery boxes. The owner has asked Shaikh to close the project on humanitarian grounds and revert back to delivery vans. However, Shaikh is confused and willing for the project to continue. Shaikh had to choose from either his own career and initiative or safety and security of employees and the company’s assets.

Complexity academic level
BBA and MBA.

Supplementary materials
Teaching Notes are available for educators only. Please contact your library to gain login details or email support@emeraldinsight.com to request teaching notes.

Subject Code
CSS 9: Operations and Logistics.
Estimating the impact of crime risk on housing prices in Malaysia
Authors: Wong Woei-Chyuan , Azhari Adilah , Abdullah Nur Adiana Hiau , Yip Chee Yin 
Published: November 2019

Purpose
The purpose of this study is to examine the impact of crime risk on housing prices at a national level in Malaysia during the period from 1988 to 2016.

Design/methodology/approach
A hedonic regression approach was used to estimate the Malaysian households’ valuation for crime risk. Specifically, the state-level property index on the state-level reported crime rate was regressed while controlling for state-level socioeconomic variables. The macroeconomic panel nature of the data set provides the merit to use a panel dynamic model instead of the traditional static panel data techniques (fixed effects or first difference).

Findings
Panel dynamic estimators consistently show a negative impact of crime risks on housing prices. The estimated elasticity of housing prices with respect to crime risks ranges from −0.141 to −0.166, in line with existing literature using micro level data. In fact, householders in crime hotspot states are willing to pay more for crime reduction compared to householders in non-hotspot states. The willingness to pay has also increased since the implementation of nationwide crime reduction plans in 2010.

Research limitations/implications
This is the first study that has examined the Malaysian people’s willingness to pay to reduce crime. This information is important in determining the optimal level of government expenditures for public safety.

Originality/value
This is the first study to examine the relationship between crime rates and housing prices in Malaysia. This study contributes to the literature by examining the impact of crime rates on housing prices at a national level by using panel dynamic models. The macro level data results are consistent and complement the existing literature based on micro level data.
Building conservation and the circular economy: a theoretical consideration
Authors: Huuhka Satu , Vestergaard Inge 
Published: November 2019

Purpose
The purpose of this paper is to discuss the relation between building conservation and circular economy (CE), which are often erroneously seen as inherently contradictory to one another.

Design/methodology/approach
The work draws from a comparative approach. The paper reviews a body of literature on architectural conservation and CE to establish an understanding on the state-of-the-art for both disciplines separately. Then, the relation between thereof is developed through a theoretical discourse.

Findings
Both architectural conservation and CE aim at safeguarding value, although they define “value” differently. Fabric-focused conservation and CE favor minimal intervention to material, albeit they arrive at this conclusion from different bases. Consequently, both approaches struggle with the low cost of virgin resource extraction and waste production and the high cost of human labor in contemporary Western societies. CE could be harnessed for building conservation by adopting its vocabulary and methodology, such as lifecycle assessment and material flow analysis. Transitioning toward CE can help increase the preservation of built heritage while redefining what is meant by “heritage” and “waste.”

Originality/value
Prior to this paper, there have been no articles addressing the relationship of the concepts explicitly and to this extent. The paper provides a theoretical basis for further discourse and outlines some implications of CE for the construction and built heritage disciplines.
Flow effect and resonance effect: group emotional contagion in service failure encounters
Authors: Du Jiangang , Yang Mengya , Liu Jianhua 
Published: November 2019

Purpose
The purpose of this paper is to explore the two effects (flow effect and resonance effect) during a group complaint based on the emotional contagion theory.

Design/methodology/approach
This study uses an experimental research design in which participants’ negative emotions dynamically change driven by group emotional interactions when they are experiencing a group complaint.

Findings
Flow effect and resonance effect can occur during the process of group emotional contagion. Specifically, when group customers’ negative emotional similarity is low in a group complaint, group emotional contagion leads to flow effect (i.e. negative emotions flow from customers with higher levels of negative emotions to those with lower levels of negative emotions). By contrast, when group customers’ negative emotional similarity is high in a group complaint, group emotional contagion leads to resonance effect (i.e. group customers’ negative emotions increase significantly).

Originality/value
Most of the previous research studies the process of emotional contagion from one with higher levels of emotional displays to the other with lower levels of emotional displays, which is named as the “flow effect” of emotional contagion. However, when two individuals with the same levels of negative emotional displays interact with each other, the flow effect of emotional contagion is very likely not to occur. It is interesting to find that both individuals’ negative emotions increase significantly during the process of emotional contagion. The authors propose the “resonance effect” of emotional contagion to explain this phenomenon.
Forklift truck performance simulation and fuel consumption estimation
Authors: d'Apolito Luigi , Hong Hanchi 
Published: November 2019

Purpose
Forklift trucks are generally operated with frequent accelerations and stops, reverse and operations of load handling. This way of operation increases the energy losses and consequently the need for reduction of fuel consumption from forklift customers. This study aims to build a model to replicate the performance of forklifts during real operations and estimate fuel consumption without building a real prototype.

Design/methodology/approach
AVL Cruise has been used to simulate forklift powertrain and hydraulic circuit. The driving cycles used for this study were in accordance with the standard VDI 2198. Artificial neural networks (ANNs), trained by the results of AVL Cruise simulations, have been used to forecast the fuel consumption for a large set of possible driving cycles.

Findings
The comparison between simulated and experimental data verified that AVL Cruise model was able to simulate the performance of real forklifts, but the results were only valid for the specified driving cycle. The ANNs, trained by the results of AVL Cruise for a certain number of driving cycles, have been found effective to forecast the fuel consumption of a larger number of driving cycles following the prescriptions of the standard VDI 2198.

Originality/value
A new method based on ANN, trained by AVL Cruise simulation results, has been introduced to forecast the forklift fuel consumption, reducing the computational time and the cost of experimental tests.
Personalized TMS: role of RNA genotyping
Authors: Chan Shawna , Bota Robert 
Published: November 2019

PurposeNoninvasive brain stimulation (NIBS) such a transcranial magnetic stimulation, intermittent theta burst stimulation, transcranial direct current stimulation and electroconvulsive therapy have emerged as an efficacious and well-tolerated therapy for treatment-resistant psychiatric disorders. While novel NIBS techniques are an exciting addition to the current repertoire of neuropsychiatric therapies, their success is somewhat limited by the wide range of treatment responses seen among treated patients.Design/methodology/approachIn this study, the authors will review the studies on relevant genetic polymorphisms and discuss the role of RNA genotyping in personalizing NIBS.FindingsGenome studies have revealed several genetic polymorphisms that may contribute for the heterogeneity of treatment response to NIBS where the presence of certain single nucleotide polymorphisms (SNPs) are associated with responders versus nonresponders.Originality/valueHistorically, mental illnesses have been arguably some of the most challenging disorders to study and to treat because of the degree of biological variability across affected individuals, the role of genetic and epigenetic modifications, the diversity of clinical symptomatology and presentations and the interplay with environmental factors. In lieu of these challenges, there has been a push for personalized medicine in psychiatry that aims to optimize treatment response based on one’s unique characteristics.
Management style in kibbutz industries
Authors: Moskovich Yaffa 
Published: November 2019

Purpose
This paper aims to develop a managerial style typology relevant to kibbutz industry analysis and applicable to all cooperative organizations.

Design/methodology/approach
This study applied qualitative methods to evaluate the organizational biographies of Factories five factories, using open interviews and document analysis.

Findings
The findings show that before privatization took place, these industries were managed according to socialistic democratic principles. Once they became global and capitalistic, some kibbutz industries adopted a business cooperative style that combines features of capitalism and socialism, while others underwent a crisis and opted for a stricter and more bureaucratic managerial style.

Research limitations/implications
This research is based on five case studies; further research is recommended to establish the current typology.

Practical implications
This study shows very clearly that the cooperative business style can be offered for businesses previously operated according to socialistic principles.

Originality/value
This study augments current literature by elucidating the speed with which business activity is conducted according to cooperative principles. It presents a typology relevant to kibbutz industry and cooperative organizations alike, addressing the cooperative managerial, cooperative business and bureaucratic styles, enabling maintenance of normative management that adapts itself to global and capitalistic environments.
Creditworthiness and access to finance: a study of SMEs in the Malaysian manufacturing industry
Authors: Wasiuzzaman Shaista , Nurdin Nabila , Abdullah Aznur Hajar , Vinayan Gowrie 
Published: November 2019

PurposeThe purpose of this study is to empirically assess the relationship between the dimensions of creditworthiness and access to finance of small and medium-sized enterprises (SMEs) in Malaysia, with creditworthiness as the mediating variable. The lack of empirical research on the relationship between creditworthiness and access to finance forms the motivation of this study.Design/methodology/approachQuestionnaires covering various characteristics of the firms, their access to finance and creditworthiness were distributed to a total of 456 SMEs in the Kuala Lumpur and Selangor region for this purpose. A total of 158 responses were returned, of which 145 were usable responses and the relationships are tested using SEM-PLS.FindingsThis study finds that an SME and its owner’s character have significant influences on access to finance. An SME’s condition and its ability to provide high quality collateral are found to be highly significant in influencing its access to finance. Capacity is significant but its significance is low, while capital is insignificant. Creditworthiness has a significant positive influence on access to finance.Originality/valueThis study contributes to the important yet under-researched issue of access to finance for SMEs. It highlights the issue of character of applicant as an important dimension of creditworthiness that can significantly influence access to finance for SMEs.