bluuebunny/crossref_metadata_2025_split huggingface dataset:
An integrated multi-omics approach identifies the landscape of interferon-α-mediated responses of human pancreatic beta cells
Authors: Colli Maikel L. , Ramos-Rodríguez Mireia , Nakayasu Ernesto S. , Alvelos Maria I. , Lopes Miguel , Hill Jessica L. E. , Turatsinze Jean-Valery , Coomans de Brachène Alexandra , Russell Mark A. , Raurell-Vila Helena , Castela Angela , Juan-Mateu Jonàs , Webb-Robertson Bobbie-Jo M. , Krogvold Lars , Dahl-Jorgensen Knut , Marselli Lorella , Marchetti Piero , Richardson Sarah J. , Morgan Noel G. , Metz Thomas O. , Pasquali Lorenzo , Eizirik Décio L. 
Published: May 2020

AbstractInterferon-α (IFNα), a type I interferon, is expressed in the islets of type 1 diabetic individuals, and its expression and signaling are regulated by T1D genetic risk variants and viral infections associated with T1D. We presently characterize human beta cell responses to IFNα by combining ATAC-seq, RNA-seq and proteomics assays. The initial response to IFNα is characterized by chromatin remodeling, followed by changes in transcriptional and translational regulation. IFNα induces changes in alternative splicing (AS) and first exon usage, increasing the diversity of transcripts expressed by the beta cells. This, combined with changes observed on protein modification/degradation, ER stress and MHC class I, may expand antigens presented by beta cells to the immune system. Beta cells also up-regulate the checkpoint proteins PDL1 and HLA-E that may exert a protective role against the autoimmune assault. Data mining of the present multi-omics analysis identifies two compound classes that antagonize IFNα effects on human beta cells.
Kinesin-14 motors drive a right-handed helical motion of antiparallel microtubules around each other
Authors: Mitra Aniruddha , Meißner Laura , Gandhimathi Rojapriyadharshini , Renger Roman , Ruhnow Felix , Diez Stefan 
Published: May 2020

AbstractWithin the mitotic spindle, kinesin motors cross-link and slide overlapping microtubules. Some of these motors exhibit off-axis power strokes, but their impact on motility and force generation in microtubule overlaps has not been investigated. Here, we develop and utilize a three-dimensional in vitro motility assay to explore kinesin-14, Ncd, driven sliding of cross-linked microtubules. We observe that free microtubules, sliding on suspended microtubules, not only rotate around their own axis but also move around the suspended microtubules with right-handed helical trajectories. Importantly, the associated torque is large enough to cause microtubule twisting and coiling. Further, our technique allows us to measure the in situ spatial extension of the motors between cross-linked microtubules to be about 20 nm. We argue that the capability of microtubule-crosslinking kinesins to cause helical motion of overlapping microtubules around each other allows for flexible filament organization, roadblock circumvention and torque generation in the mitotic spindle.
Vesicular transport mediates the uptake of cytoplasmic proteins into mitochondria in Drosophila melanogaster
Authors: Chen Po-Lin , Huang Kai-Ting , Cheng Chu-Ya , Li Jian-Chiuan , Chan Hsiao-Yen , Lin Tzu-Yang , Su Matthew P. , Yang Wei-Yuan , Chang Henry C. , Wang Horng-Dar , Chen Chun-Hong 
Published: May 2020

AbstractMitochondrial aging, which results in mitochondrial dysfunction, is strongly linked to many age-related diseases. Aging is associated with mitochondrial enlargement and transport of cytosolic proteins into mitochondria. The underlying homeostatic mechanisms that regulate mitochondrial morphology and function, and their breakdown during aging, remain unclear. Here, we identify a mitochondrial protein trafficking pathway in Drosophila melanogaster involving the mitochondria-associated protein Dosmit. Dosmit induces mitochondrial enlargement and the formation of double-membraned vesicles containing cytosolic protein within mitochondria. The rate of vesicle formation increases with age. Vesicles originate from the outer mitochondrial membrane as observed by tracking Tom20 localization, and the process is mediated by the mitochondria-associated Rab32 protein. Dosmit expression level is closely linked to the rate of ubiquitinated protein aggregation, which are themselves associated with age-related diseases. The mitochondrial protein trafficking route mediated by Dosmit offers a promising target for future age-related mitochondrial disease therapies.
Gestational alcohol exposure disrupts cognitive function and striatal circuits in adult offspring
Authors: Cuzon Carlson Verginia C. , Gremel Christina M. , Lovinger David M. 
Published: May 2020

AbstractFetal alcohol exposure (FAE) is the leading preventable developmental cause of cognitive dysfunction. Even in the absence of binge drinking, alcohol consumption during pregnancy can leave offspring deficient. However, the mechanisms underlying these deficiencies are unknown. Using a mouse model of gestational ethanol exposure (GEE), we show increased instrumental lever-pressing and disruption of efficient habitual actions in adults, indicative of disrupted cognitive function. In vivo electrophysiology reveals disrupted action encoding in dorsolateral striatum (DLS) associated with altered habit learning. GEE mice exhibit decreased GABAergic transmission onto DLS projection neurons, including inputs from parvalbumin interneurons, and increased endocannabinoid tone. Chemogenetic activation of DLS parvalbumin interneurons reduces the elevated lever pressing of GEE mice. Pharmacologically increasing endocannabinoid tone mimics GEE effects on cognition and synaptic transmission. These findings show GEE induces long-lasting deficits in cognitive function that may contribute to human FAE, and identify potential mechanisms for future therapeutic targeting.
Methylation of a CGATA element inhibits binding and regulation by GATA-1
Authors: Yang Lu , Chen Zhiliang , Stout Elizabeth S. , Delerue Fabien , Ittner Lars M. , Wilkins Marc R. , Quinlan Kate G. R. , Crossley Merlin 
Published: May 2020

AbstractAlterations in DNA methylation occur during development, but the mechanisms by which they influence gene expression remain uncertain. There are few examples where modification of a single CpG dinucleotide directly affects transcription factor binding and regulation of a target gene in vivo. Here, we show that the erythroid transcription factor GATA-1 — that typically binds T/AGATA sites — can also recognise CGATA elements, but only if the CpG dinucleotide is unmethylated. We focus on a single CGATA site in the c-Kit gene which progressively becomes unmethylated during haematopoiesis. We observe that methylation attenuates GATA-1 binding and gene regulation in cell lines. In mice, converting the CGATA element to a TGATA site that cannot be methylated leads to accumulation of megakaryocyte-erythroid progenitors. Thus, the CpG dinucleotide is essential for normal erythropoiesis and this study illustrates how a single methylated CpG can directly affect transcription factor binding and cellular regulation.
Mott gap collapse in lightly hole-doped Sr2−xKxIrO4
Authors: Nelson J. N. , Parzyck C. T. , Faeth B. D. , Kawasaki J. K. , Schlom D. G. , Shen K. M. 
Published: May 2020

AbstractThe evolution of Sr2IrO4 upon carrier doping has been a subject of intense interest, due to its similarities to the parent cuprates, yet the intrinsic behaviour of Sr2IrO4 upon hole doping remains enigmatic. Here, we synthesize and investigate hole-doped Sr2−xKxIrO4 utilizing a combination of reactive oxide molecular-beam epitaxy, substitutional diffusion and in-situ angle-resolved photoemission spectroscopy. Upon hole doping, we observe the formation of a coherent, two-band Fermi surface, consisting of both hole pockets centred at (π, 0) and electron pockets centred at (π/2, π/2). In particular, the strong similarities between the Fermi surface topology and quasiparticle band structure of hole- and electron-doped Sr2IrO4 are striking given the different internal structure of doped electrons versus holes.
Selective C-H trifluoromethoxylation of (hetero)arenes as limiting reagent
Authors: Deng Zhijie , Zhao Mingxin , Wang Feng , Tang Pingping 
Published: May 2020

AbstractMethods for direct C-H trifluoromethoxylation of arenes and heteroarenes are rare, despite the importance of trifluoromethoxylated compounds for pharmaceuticals, agrochemicals, and material sciences. Especially selective C-H trifluoromethoxylation of pyridines remains a formidable challenge. Here we show a general late-stage C-H trifluoromethoxylation of arenes and heteroarenes as limiting reagent with trifluoromethoxide anion. The reaction is mediated by silver salts under mild reaction conditions, exhibiting broad substrate scope and wide functional-group compatibility. In addition, ortho-position selective C-H trifluoromethoxylation of pyridines is observed. The method is not only applicable to the gram-scale synthesis of trifluoromethoxylated products but also allows efficient late-stage C-H trifluoromethoxylation of marketed small-molecule drugs, common pharmacophores and natural products.
Activation of allylic esters in an intramolecular vinylogous kinetic resolution reaction with synergistic magnesium catalysts
Authors: Li Dan , Yang Yuling , Zhang Minmin , Wang Linqing , Xu Yingfan , Yang Dongxu , Wang Rui 
Published: May 2020

AbstractKinetic resolution (KR) of racemic starting materials is a powerful and practical alternative to prepare valuable enantiomerically enriched compounds. A magnesium-catalyzed kinetic resolution based on a designed intramolecular vinylogous Michael reaction is disclosed. Here we show a synergistic catalytic strategy based on the development of chiral ligands. Substrates containing linear allylic ester structures are designed and synthesized to construct key [6.6.5]-tricyclic chiral skeletons via this kinetic resolution process. Detailed mechanistic studies reveal a rational mechanism for the current intramolecular vinylogous KR reaction. The desired direct intramolecular asymmetric vinylogous Michael reaction of linear allylic esters is realized in high efficiency and enantioselectivity with the synergistic catalytic system.
Continuous monitoring of suspended sediment concentrations using image analytics and deriving inherent correlations by machine learning
Authors: Ghorbani Mohammad Ali , Khatibi Rahman , Singh Vijay P. , Kahya Ercan , Ruskeepää Heikki , Saggi Mandeep Kaur , Sivakumar Bellie , Kim Sungwon , Salmasi Farzin , Hasanpour Kashani Mahsa , Samadianfard Saeed , Shahabi Mahmood , Jani Rasoul 
Published: May 2020

AbstractThe barriers for the development of continuous monitoring of Suspended Sediment Concentration (SSC) in channels/rivers include costs and technological gaps but this paper shows that a solution is feasible by: (i) using readily available high-resolution images; (ii) transforming the images into image analytics to form a modelling dataset; and (iii) constructing predictive models by learning inherent correlation between observed SSC values and their image analytics. High-resolution images were taken of water containing a series of SSC values using an exploratory flume. Machine learning is processed by dividing the dataset into training and testing sets and the paper uses the following models: Generalized Linear Machine (GLM) and Distributed Random Forest (DRF). Results show that each model is capable of reliable predictions but the errors at higher SSC are not fully explained by modelling alone. Here we offer sufficient evidence for the feasibility of a continuous SSC monitoring capability in channels before the next phase of the study with the goal of producing practice guidelines.
Fleeting Perceptual Experience and the Possibility of Recalling Without Seeing
Authors: Jones William , Pincham Hannah , Gootjes-Dreesbach Ellis Luise , Bowman Howard 
Published: May 2020

AbstractWe explore an intensely debated problem in neuroscience, psychology and philosophy: the degree to which the “phenomenological consciousness” of the experience of a stimulus is separable from the “access consciousness” of its reportability. Specifically, it has been proposed that these two measures are dissociated from one another in one, or both directions. However, even if it was agreed that reportability and experience were doubly dissociated, the limits of dissociation logic mean we would not be able to conclusively separate the cognitive processes underlying the two. We take advantage of computational modelling and recent advances in state-trace analysis to assess this dissociation in an attentional/experiential blink paradigm. These advances in state-trace analysis make use of Bayesian statistics to quantify the evidence for and against a dissociation. Further evidence is obtained by linking our finding to a prominent model of the attentional blink – the Simultaneous Type/Serial Token model. Our results show evidence for a dissociation between experience and reportability, whereby participants appear able to encode stimuli into working memory with little, if any, conscious experience of them. This raises the possibility of a phenomenon that might be called sight-blind recall, which we discuss in the context of the current experience/reportability debate.
Expression of genes of the Pho regulon is altered in Streptomyces coelicolor
Authors: Millan-Oropeza Aaron , Henry Céline , Lejeune Clara , David Michelle , Virolle Marie-Joelle 
Published: May 2020

AbstractMost currently used antibiotics originate from Streptomycetes and phosphate limitation is an important trigger of their biosynthesis. Understanding the molecular processes underpinning such regulation is of crucial importance to exploit the great metabolic diversity of these bacteria and get a better understanding of the role of these molecules in the physiology of the producing bacteria. To contribute to this field, a comparative proteomic analysis of two closely related model strains,Streptomyces lividansandStreptomyces coelicolorwas carried out. These strains possess identical biosynthetic pathways directing the synthesis of three well-characterized antibiotics (CDA, RED and ACT) but onlyS. coelicolorexpresses them at a high level. Previous studies established that the antibiotic producer,S. coelicolor, is characterized by an oxidative metabolism and a reduced triacylglycerol content compared to the none producer,S. lividans, characterized by a glycolytic metabolism. Our proteomic data support these findings and reveal that these drastically different metabolic features could, at least in part, due to the weaker abundance of proteins of the two component system PhoR/PhoP inS. coelicolorcompared toS. lividans. In condition of phosphate limitation, PhoR/PhoP is known to control positively and negatively, respectively, phosphate and nitrogen assimilation and our study revealed that it might also control the expression of some genes of central carbon metabolism. The tuning down of the regulatory role of PhoR/PhoP inS. coelicoloris thus expected to be correlated with low and high phosphate and nitrogen availability, respectively and with changes in central carbon metabolic features. These changes are likely to be responsible for the observed differences betweenS. coelicolorandS. lividansconcerning energetic metabolism, triacylglycerol biosynthesis and antibiotic production. Furthermore, a novel view of the contribution of the bio-active molecules produced in this context, to the regulation of the energetic metabolism of the producing bacteria, is proposed and discussed.
First evidence of a palaeo-nursery area of the great white shark
Authors: Villafaña Jaime A. , Hernandez Sebastian , Alvarado Alonso , Shimada Kenshu , Pimiento Catalina , Rivadeneira Marcelo M. , Kriwet Jürgen 
Published: May 2020

AbstractShark nurseries are essential habitats for shark survival. Notwithstanding the rich fossil record of the modern great white shark (Carcharodon carcharias, GWS), its use of nursery areas in the fossil record has never been assessed before. Here, we analysed the fossil record of the GWS from three South American Pliocene localities, assessed body size distributions and applied previously established criteria to identify palaeo-nurseries. We found that juveniles dominate the Coquimbo locality (Chile), whereas subadults and adults characterize Pisco (Peru) and Caldera (Chile), respectively. These results, summed to the paleontological and paleoenvironmental record of the region, suggest that Coquimbo represents the first nursery area for the GWS in the fossil record. Our findings demonstrate that one of the top predators in today’s oceans has used nursery areas for millions of years, highlighting their importance as essential habitats for shark survival in deep time.
A new approach to prevent cervical stenosis in postmenopausal women after loop electrosurgical excision procedure: a randomized controlled trial
Authors: Lin Jing , Meng Yu , Chen Yi , Li Zhunan , Xu Ying , Wu Dan 
Published: May 2020

AbstractTo determine whether regular cervical dilatation is effective for preventing cervical stenosis, and to identify the associated risk factors, in postmenopausal women after LEEP. This was a prospective randomized clinical trial in postmenopausal women who underwent LEEP at our hospital between August 2018 and May 2019. Patients who met the study criteria were randomly allocated to three groups: control group (without any intervention), intervention group A (underwent cervical dilatation at the 3rd, 5th, and 8th week after LEEP) and intervention group B (underwent cervical dilatation at the 4th, 8th, and 12th week after LEEP). A colposcopic follow-up examination was conducted at 6 months after LEEP to determine the incidence of cervical stenosis. A total of 404 postmenopausal women were found to be finally eligible for the study. The rate of cervical stenosis in the control group was significantly higher than that in the intervention group, and the rate in group A was significantly lower than that in group B. We found regular dilatation after LEEP in postmenopausal women can prevent cervical stenosis. Further, the 3rd, 5th, and 8th weeks after LEEP are optimal time points. Finally, LEEP frequency and resection depth are significant risk factors and can be used to screen postmenopausal women at risk for cervical stenosis after LEEP.
Non-mantle-plume process caused the initial spreading of the South China Sea
Authors: Yu Xun , Liu Zhifei 
Published: May 2020

AbstractThe mantle plume process is thought to be the prevailing dynamic mechanism for the South China Sea opening, but controversy persists due to the lack of critical evidence of magma in the initial seafloor spreading. International Ocean Discovery Program (IODP) Expedition 367 successfully recovered at Site U1500 the mid-ocean ridge basalt (MORB) representing the magma activity of the initial spreading of the South China Sea during the earliest Oligocene. Here we present the whole-rock and olivine phenocryst geochemistry of the basalts to constrain the potential influence of the Hainan mantle plume on the evolution of the South China Sea. Major and trace elemental compositions indicate that the basalts were mainly influenced by fractional crystallization of olivine and formed by melting of a spinel peridotite source without any pyroxenite in mantle source. The calculated mantle potential temperature of those most primitive basalts is much lower than plume-related MORB of Iceland, but similar to normal MORB elsewhere. Both lithological composition and mantle potential temperature clearly contradict with the mantle plume model, signifying that the mantle plume didn’t exist at the earliest Oligocene. Therefore, the initial spreading of the South China Sea should be caused by non-plume processes, most likely by the westward subduction of the Pacific Plate.
Influence of garlic and pepper powder on physicochemical and sensory qualities of flavoured rice noodle
Authors: Utama-ang Niramon , Cheewinworasak Thosaphon , Simawonthamgul Natthawut , Samakradhamrongthai Rajnibhas Sukeaw 
Published: May 2020

AbstractThis research aimed to investigate the effect of dried garlic powder (DGP) and dried white pepper powder (DWPP) on physicochemical and sensory properties and to develop a garlic-pepper flavoured purple rice noodle (GPFRD). The garlic-pepper powder (GPP) aroma found to be comprised of pine, garlic, onion, citrus and woody characteristic. The 78 g of DGP and 20 g of DWPP provided high sensory rating score of pepper aroma (5.9 ± 0.1) and overall aroma (5.6 ± 0.2) with a high content of beta-caryophyllene (0.101 ± 0.04 mg/g powder), limonene (0.069 ± 0.02 mg/g powder), allicin (10.48 ± 0.18 mg/g powder) and piperine (0.71 ± 0.11 mg/g powder). The cooked GPFRD mixed with garlic-pepper at 2% possessed the good quality of physical and chemical properties with sensory rating score. The GPFRD using 2% of GPP provided preference rating score in the range of 6.0–6.7 with consumer acceptance at 82.0% and purchase intention at 74.0%. Consequently, the optimum ratio of DGP and DWPP provided a better spice mix for aroma, flavour, with some bioactive compound aspects. A suitable amount of GPP can provide the preferable properties of flavoured purple rice noodles.
Independent and combined effects of age, body mass index and gestational weight gain on the risk of gestational diabetes mellitus
Authors: Yong Heng Yaw , Mohd Shariff Zalilah , Mohd Yusof Barakatun Nisak , Rejali Zulida , Tee Yvonne Yee Siang , Bindels Jacques , van der Beek Eline M. 
Published: May 2020

AbstractThis study aimed to identify the independent and combined effects of age, BMI at first prenatal visit and GWG on the risk of GDM. A retrospective cohort study of 1,951 pregnant women in Seremban district, Negeri Sembilan, Malaysia. GDM was defined as fasting plasma glucose (FPG) ≥5.6 mmol/l and/or 2-hour postprandial plasma glucose (2hPPG) ≥7.8 mmol/l. A higher percentage of women with GDM had 2 risk factors (29.0%) or >2 risk factors (8.6%) compared to non-GDM women (2 risk factors: 25.5%; >2 risk factors: 5.0%). In general, women with ≥2 risk factors were respectively 1.36–2.06 times more likely to have GDM compared to those without risk factors. Older maternal age and being overweight/obese were significantly associated with risk of GDM. Overweight/obese women with age ≥35 years had 2.45 times higher risk of GDM and having excessive GWG at second trimester further increased the risk of GDM. Age and BMI are independent risk factors for GDM but not GWG in the first and second trimester. The findings emphasize the need to focus on a healthy BMI before pregnancy and optimal GWG during pregnancy to improve pregnancy outcomes.
Effect of SWCNT and MWCNT on the flow of micropolar hybrid nanofluid over a curved stretching surface with induced magnetic field
Authors: Al-Hanaya A. M. , Sajid Farrah , Abbas Nadeem , Nadeem S. 
Published: May 2020

AbstractWe considered the magnetized micro polar fluid with hybrid nanomaterial flow over a curved stretching surface. We discussed the effects of single wall carbon nanotube and multiwall carbon nanotube with base fluids (water and propylene glycol). Under the flow assumptions, we developed the mathematical model and applied the boundary layer approximations to reduce the system of partial differential equations. Further, the suitable similarity transformations are applied on the partial differential equations to make dimensionless system. The dimensionless system solved by means of numerical scheme via bvp4c shooting methods. Involving the dimensionless physical parameters effects are highlighted in the form of graphs and tables. Additionally, significant physical quantities i.e. Nusselt number, Couple stress coefficient and Skin friction coefficient are also presented and evaluated numerically. These results are more important which may use in the field of engineering and industrial.