bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Ups and Downs of Daily Life During COVID-19: Age Differences in Affect, Stress, and Positive Events
Authors: Klaiber Patrick , Wen Jin H , DeLongis Anita , Sin Nancy L 
Published: July 2020

Abstract

Objectives
Past research has linked older age with greater emotional well-being and decreased reactivity to stressors, but it is unknown whether age-related advantages in emotional well-being are maintained in the wake of COVID-19. We examined age differences in exposure and affective reactivity to daily stressors and positive events in the first several weeks of the COVID-19 outbreak.

Methods
In March and April 2020, 776 adults from Canada and the United States aged 18–91 (mean age 45) years reported COVID-19 threats at baseline, then completed nightly surveys for 1 week about their daily stressors, positive events, and affect.

Results
Younger age predicted more concerns about the threat of COVID-19 across multiple domains, in addition to lower positive affect, higher negative affect, and less frequent positive events. Younger adults had more non-COVID-19 daily stressors and higher perceived control over stressors, but lower perceived coping efficacy than older adults. There were no age differences in the frequency of COVID-19 daily stressors nor perceived stressor severity. Younger adults had greater reductions in negative affect on days when more positive events occurred and greater increases in negative affect on days when non-COVID-19 stressors occurred. Age moderation was attenuated for negative affective reactivity to COVID-19 stressors. Age did not moderate positive affective reactivity to daily events.

Discussion
In the early weeks of the pandemic, older adults showed better emotional well-being and less reactivity to stressors but did not differ from younger adults in their exposure to COVID-19 stressors. Additionally, younger adults benefited more from positive events.
Effect of human papilloma virus vaccination on sexual behaviours among adolescent women in Rwanda: a regression discontinuity study
Authors: Hategeka Celestin , Ogilvie Gina , Nisingizwe Marie Paul , Rulisa Stephen , Law Michael R 
Published: July 2020

Abstract
Increasing human papilloma virus (HPV) vaccination coverage is one of the key approaches to preventing cervical cancer globally. However, some argue that HPV vaccine recipients may engage in risky compensatory sexual behaviours because of perceived protection afforded by the vaccine. Therefore, we investigated the impact of a wide-scale HPV vaccination programme on sexual behaviours among adolescent women in Rwanda—the first African country to implement a national HPV vaccination. We identified a cohort of women who were eligible for the HPV vaccination and those who were not eligible from the most recent Rwanda Demographic and Health Survey. We used a quasi-experimental regression discontinuity design, exploiting the quasi-random change in HPV vaccination eligibility in 2011, to compare sexual behaviours among vaccinated and unvaccinated adolescent women. We studied the impact of the vaccination on reported sexual intercourse, average number of sexual partners and teenage pregnancy across the vaccination eligibility threshold. Our analysis included 3052 adolescent women (mean age: 18.6 years), of whom 58% were eligible for HPV vaccination. Nearly one in five adolescents reported having had sexual intercourse (18.5%). The average reported lifetime number of sexual partners was 1.41. The proportion of teenage pregnancy was 5.3%. We found no evidence that HPV vaccination was associated with any significant changes across the eligibility threshold in reported sexual behaviours we studied: no significant increase in the proportion of having sexual intercourse [odds ratio (OR): 0.80, 95% confidence interval (CI): 0.57–1.12; P = 0.19], in lifetime number of sexual partners (rate ratio 0.99, 95% CI: 0.83–1.17, P = 0.91) and in the proportion of teen pregnancies (OR 1.05, 95% CI: 0.50 to 2.20, P = 0.89) at the eligibility threshold. The Rwandan national HPV vaccination programme did not increase sexual behaviours among adolescent women, assuaging concerns of engaging in risky compensatory sexual behaviours some have feared.
Catching up through green windows of opportunity in an era of technological transformation: Empirical evidence from the Chinese wind energy sector
Authors: Dai Yixin , Haakonsson Stine , Oehler Lars 
Published: July 2020

AbstractRecent transformations in the global wind energy industry have considerable implications for firms to catch up as the sectoral frontier advances from on- and offshore wind turbines towards digital/hybrid systems. These technological shifts potentially precipitate new green windows of opportunity. This article finds that latecomer firms show different capabilities in responding to technological transformation at the global level, which explains variations in catch-up trajectories under the same framework conditions.
Evaluating acoustic-trawl survey strategies using an end-to-end ecosystem model
Authors: Holmin Arne Johannes , Mousing Erik A , Hjøllo Solfrid S , Skogen Morten D , Huse Geir , Handegard Nils Olav 
Published: July 2020

AbstractFisheries independent surveys support science and fisheries assessments but are costly. Evaluating the efficacy of a survey before initiating it could save costs. We used the NORWECOM.E2E model to simulate Northeast Atlantic mackerel and Norwegian spring spawning herring distributions in the Norwegian Sea, and we ran vessel transects in silico to simulate acoustic-trawl surveys. The simulated data were processed using standard survey estimation software and compared to the stock abundances in the ecosystem model. Three existing real surveys were manipulated to demonstrate how the simulation framework can be used to investigate effects of changes in survey timing, direction, and coverage on survey estimates. The method picked up general sources of biases and variance, i.e. that surveys conducted during fish migrations are more vulnerable in terms of bias to timing and changes in survey direction than during more stationary situations and that increased effort reduced the sampling variance.
How Concrete Language Shapes Customer Satisfaction
Authors: Packard Grant , Berger Jonah 
Published: July 2020

Abstract
Consumers are often frustrated by customer service. But could a simple shift in language help improve customer satisfaction? We suggest that linguistic concreteness—the tangibility, specificity, or imaginability of words employees use when speaking to customers—can shape consumer attitudes and behaviors. Five studies, including text analysis of over 1,000 real consumer–employee interactions in two different field contexts, demonstrate that customers are more satisfied, willing to purchase, and purchase more when employees speak to them concretely. This occurs because customers infer that employees who use more concrete language are listening (i.e., attending to and understanding their needs). These findings deepen understanding of how language shapes consumer behavior, reveal a psychological mechanism by which concreteness impacts person perception, and provide a straightforward way that managers could help enhance customer satisfaction.
A rare case of duplicated appendix presenting as perforated appendicitis
Authors: Raja A Ashwin , Vasanthakumaran Tamilarasy 
Published: July 2020

Abstract
Anomalies of the appendix have been reported mostly in adults as an incidental finding during surgery. The incidence of duplication of the vermiform appendix is reported to be 0.004% and &lt;10 cases of appendicitis have been reported in duplicated appendix. This is a case report of an 18-year-old male who was diagnosed to have perforated appendicitis by imaging, and on open surgical exploration was found to have appendiceal duplication. Histopathological examination of both specimens revealed a perforated necrotic first appendix and an acutely inflamed second appendix. Surgeons must always bear in mind a high degree of suspicion regarding the possibility of duplicated appendices in all cases that are clinically and radiologically suspected to have acute appendicitis or perforated appendicitis. A thorough intraoperative examination of caecum is mandatory to avoid missing the duplication; thus, preventing post-operative complications and medicolegal issues.
Titanium clip migration after cholecystectomy: original technique for laparoscopic transcystic extraction when endoscopic retrograde cholangiopancreatography fails
Authors: Pantoja Pachajoa Diana A , Bruno Marco A , Doniquian Alejandro M , Alvarez Fernando A 
Published: July 2020

Abstract
Surgical clip migration into the common bile duct (CBD) is a rare complication after laparoscopic cholecystectomy (LC). Few cases of surgical clip migration have been reported in the literature, and most of them have been successfully treated with endoscopic retrograde cholangiopancreatography (ERCP). We present a 71-year-old woman with 48 h of abdominal pain, jaundice and fever 6 years after laparoscopic cholecystectomy. She was diagnosed with common bile duct obstruction from surgical clip migration. After failure of ERCP, the patient was successfully treated with an innovative approach by laparoscopic transcystic extraction using endoscopic hose-type biopsy forceps. The presented technique was feasible and safe in expert hands, representing a valuable alternative to avoid the need of a choledochotomy in patients with unsuccessful ERCP.
Corynebacterium diphtheriae Infection in Mahajanga, Madagascar: First Case Report
Authors: Rakotomalala Rivo Solotiana , Andrianirina Zo Zafitsara , Ratsima Elisoa , Randrianandraina Patrick , Randrianirina Frédérique , Edosoa Glenn Torrencelli , Rabenandrianina Tahirimalala , Badell Edgar , Toubiana Julie , Andrianarimanana Diavolana , Brisse Sylvain , Rasamindrakotroka Andry 
Published: July 2020

Abstract
Diphtheria is an infection that has been unreported for more than two decades in Mahajanga. A child, aged 4, presented with a pseudomembranous pharyngitis was associated with a dysphagia. He was from a rural municipality of Ambato Boeny at Mahajanga province and was admitted to the Pediatric Unit of the University Hospital Center. The child was not immunized against diphtheria. A throat swab was performed and cultured, from which Corynebacterium diphtheriae was identified. The strain, of biovar Mitis, was confirmed as diphtheria toxin (DT)-gene positive and produced DT (Elek test). Unfortunately, the child developed cardiac and neurological complications and died of respiratory and heart failure.
The impact of global selection on local adaptation and reproductive isolation
Authors: Bisschop Gertjan , Setter Derek , Rafajlović Marina , Baird Stuart J. E. , Lohse Konrad 
Published: July 2020

Despite the homogenizing effect of strong gene flow between two populations, adaptation under symmetric divergent selection pressures results in partial reproductive isolation: adaptive substitutions act as local barriers to gene flow, and if divergent selection continues unimpeded, this will result in complete reproductive isolation of the two populations, i.e. speciation. However, a key issue in framing the process of speciation as a tension between local adaptation and the homogenizing force of gene flow is that the mutation process is blind to changes in the environment and therefore tends to limit adaptation. Here we investigate how globally beneficial mutations (GBMs) affect divergent local adaptation and reproductive isolation. When phenotypic divergence is finite, we show that the presence of GBMs limits local adaptation, generating a persistent genetic load at the loci that contribute to the trait under divergent selection and reducing genome-wide divergence. Furthermore, we show that while GBMs cannot prohibit the process of continuous differentiation, they induce a substantial delay in the genome-wide shutdown of gene flow.
This article is part of the theme issue ‘Towards the completion of speciation: the evolution of reproductive isolation beyond the first barriers’.
The limits to parapatric speciation 3: evolution of strong reproductive isolation in presence of gene flow despite limited ecological differentiation
Authors: Blanckaert Alexandre , Bank Claudia , Hermisson Joachim 
Published: July 2020

Gene flow tends to impede the accumulation of genetic divergence. Here, we determine the limits for the evolution of postzygotic reproductive isolation in a model of two populations that are connected by gene flow. We consider two selective mechanisms for the creation and maintenance of a genetic barrier: local adaptation leads to divergence among incipient species due to selection against migrants, and Dobzhansky–Muller incompatibilities (DMIs) reinforce the genetic barrier through selection against hybrids. In particular, we are interested in the maximum strength of the barrier under a limited amount of local adaptation, a challenge that many incipient species may initially face. We first confirm that with classical two-locus DMIs, the maximum amount of local adaptation is indeed a limit to the strength of a genetic barrier. However, with three or more loci and cryptic epistasis, this limit holds no longer. In particular, we identify a minimal configuration of three epistatically interacting mutations that is sufficient to confer strong reproductive isolation.This article is part of the theme issue ‘Towards the completion of speciation: the evolution of reproductive isolation beyond the first barriers’.
The importance of intrinsic postzygotic barriers throughout the speciation process
Authors: Coughlan Jenn M. , Matute Daniel R. 
Published: July 2020

Intrinsic postzygotic barriers can play an important and multifaceted role in speciation, but their contribution is often thought to be reserved to the final stages of the speciation process. Here, we review how intrinsic postzygotic barriers can contribute to speciation, and how this role may change through time. We outline three major contributions of intrinsic postzygotic barriers to speciation. (i)reduction of gene flow: intrinsic postzygotic barriers can effectively reduce gene exchange between sympatric species pairs. We discuss the factors that influence how effective incompatibilities are in limiting gene flow. (ii)early onset of species boundaries via rapid evolution: intrinsic postzygotic barriers can evolve between recently diverged populations or incipient species, thereby influencing speciation relatively early in the process. We discuss why the early origination of incompatibilities is expected under some biological models, and detail how other (and often less obvious) incompatibilities may also serve as important barriers early on in speciation. (iii)reinforcement: intrinsic postzygotic barriers can promote the evolution of subsequent reproductive isolation through processes such as reinforcement, even between relatively recently diverged species pairs. We incorporate classic and recent empirical and theoretical work to explore these three facets of intrinsic postzygotic barriers, and provide our thoughts on recent challenges and areas in the field in which progress can be made.This article is part of the theme issue ‘Towards the completion of speciation: the evolution of reproductive isolation beyond the first barriers’.
Can the genomics of ecological speciation be predicted across the divergence continuum from host races to species? A case study inRhagoletis
Authors: Meyers Peter J. , Doellman Meredith M. , Ragland Gregory J. , Hood Glen R. , Egan Scott P. , Powell Thomas H. Q. , Nosil Patrik , Feder Jeffrey L. 
Published: July 2020

Studies assessing the predictability of evolution typically focus on short-term adaptation within populations or the repeatability of change among lineages. A missing consideration in speciation research is to determine whether natural selection predictably transforms standing genetic variation within populations into differences between species. Here, we test whether and how host-related selection on diapause timing associates with genome-wide differentiation during ecological speciation by comparing ancestral hawthorn and newly formed apple-infesting host races ofRhagoletis pomonellato their sibling speciesRhagoletis mendaxthat attacks blueberries. The associations of 57 857 single nucleotide polymorphisms in a diapause genome-wide-association study (GWAS) on the hawthorn race strongly predicted the direction and magnitude of genomic divergence among the three fly populations at a field site in Fennville, MI, USA. The apple race andR. mendaxshow parallel changes in the frequencies of putative inversions on three chromosomes associated with the earlier fruiting times of apples and blueberries compared to hawthorns. A diapause GWAS onR. mendaxrevealed compensatory changes throughout the genome accounting for the earlier eclosion of blueberry, but not apple flies. Thus, a degree of predictability, although not complete, exists in the genomics of diapause across the ecological speciation continuum inRhagoletis. The generality of this result is placed in the context of other similar systems.This article is part of the theme issue ‘Towards the completion of speciation: the evolution of reproductive isolation beyond the first barriers'.
Evolutionary dynamics of pre- and postzygotic reproductive isolation in cichlid fishes
Authors: Rometsch Sina J. , Torres-Dowdall Julián , Meyer Axel 
Published: July 2020

Cichlid fishes are exceptionally species-rich, speciated at explosive rates and, hence, are a model system in speciation research. Yet, their reproductive isolating barriers have, so far, not been comprehensively studied. Here, we review current knowledge on pre- and postzygotic mechanisms in cichlids. While premating isolation is the norm in cichlids, its strength varies across lineages and with the geographical setting. Moreover, manipulations of ambient conditions tended to reduce assortative mating among closely related species, suggesting that premating isolation in cichlids is often fragile and context dependent. The observed lack of complete reproductive isolation is supported by past and present hybridization events that have contributed to diversity by creating novel allelic combinations. On the other hand, our meta-analysis highlights that intrinsic postzygotic isolation might accumulate faster than assumed. Mild forms of genetic incompatibilities, such as sex ratio distortion, can already be observed among closely related species. Therefore, cessation of gene flow by strong reproductive isolation in cichlids requires a combination of premating prezygotic isolation supplemented with intrinsic and extrinsic postzygotic barriers. Further, we suggest crucial next steps to improve our knowledge about reproductive barriers in cichlids to understand the evolutionary dynamics of pre- and postzygotic isolation mechanisms during adaptive radiations.This article is part of the theme issue ‘Towards the completion of speciation: the evolution of reproductive isolation beyond the first barriers'.
The role of copy-number variation in the reinforcement of sexual isolation between the two European subspecies of the house mouse
Authors: North Henry L. , Caminade Pierre , Severac Dany , Belkhir Khalid , Smadja Carole M. 
Published: July 2020

Reinforcement has the potential to generate strong reproductive isolation through the evolution of barrier traits as a response to selection against maladaptive hybridization, but the genetic changes associated with this process remain largely unexplored. Building upon the increasing evidence for a role of structural variants in adaptation and speciation, we addressed the role of copy-number variation in the reinforcement of sexual isolation evidenced between the two European subspecies of the house mouse. We characterized copy-number divergence between populations ofMus musculus musculusthat display assortative mate choice, and those that do not, using whole-genome resequencing data. Updating methods to detect deletions and tandem duplications (collectively: copy-number variants, CNVs) in Pool-Seq data, we developed an analytical pipeline dedicated to identifying genomic regions showing the expected pattern of copy-number displacement under a reinforcement scenario. This strategy allowed us to detect 1824 deletions and seven tandem duplications that showed extreme differences in frequency between behavioural classes across replicate comparisons. A subset of 480 deletions and four tandem duplications were specifically associated with the derived trait of assortative mate choice. These ‘Choosiness-associated’ CNVs occur in hundreds of genes. Consistent with our hypothesis, such genes included olfactory receptors potentially involved in the olfactory-based assortative mate choice in this system as well as one gene,Sp110, that is known to show patterns of differential expression between behavioural classes in an organ used in mate choice—the vomeronasal organ. These results demonstrate that fine-scale structural changes are common and highly variable within species, despite being under-studied, and may be important targets of reinforcing selection in this system and others.This article is part of the theme issue ‘Towards the completion of speciation: the evolution of reproductive isolation beyond the first barriers’.
Multi-locus interactions and the build-up of reproductive isolation
Authors: Satokangas I. , Martin S. H. , Helanterä H. , Saramäki J. , Kulmuni J. 
Published: July 2020

All genes interact with other genes, and their additive effects and epistatic interactions affect an organism's phenotype and fitness. Recent theoretical and empirical work has advanced our understanding of the role of multi-locus interactions in speciation. However, relating different models to one another and to empirical observations is challenging. This review focuses on multi-locus interactions that lead to reproductive isolation (RI) through reduced hybrid fitness. We first review theoretical approaches and show how recent work incorporating a mechanistic understanding of multi-locus interactions recapitulates earlier models, but also makes novel predictions concerning the build-up of RI. These include high variance in the build-up rate of RI among taxa, the emergence of strong incompatibilities producing localized barriers to introgression, and an effect of population size on the build-up of RI. We then review recent experimental approaches to detect multi-locus interactions underlying RI using genomic data. We argue that future studies would benefit from overlapping methods like ancestry disequilibrium scans, genome scans of differentiation and analyses of hybrid gene expression. Finally, we highlight a need for further overlap between theoretical and empirical work, and approaches that predict what kind of patterns multi-locus interactions resulting in incompatibilities will leave in genome-wide polymorphism data.This article is part of the theme issue ‘Towards the completion of speciation: the evolution of reproductive isolation beyond the first barriers’.