bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dynamical Roles of Mixed Rossby–Gravity Waves in Driving Convective Initiation and Propagation of the Madden–Julian Oscillation: General Views
Authors: Takasuka Daisuke , Satoh Masaki 
Published: October 2020

AbstractMotivated by the previous case study, this work shows that dynamical variations of mixed Rossby–gravity waves with tropical depression–type circulations (MRGTDs) are possible drivers of convective initiation and propagation of the Madden–Julian oscillation (MJO) by performing statistical analysis. MJO events initiated in the Indian Ocean (IO) in boreal winter are objectively identified solely using outgoing longwave radiation data. The lagged-composite analysis of detected MJO events demonstrates that MJO convection is initiated in the southwestern IO (SWIO), where strong MRGTD–convection coupling is statistically found. Further classification of MJO cases in terms of intraseasonal convection and MRGTD activities in the SWIO suggests that 26 of 47 cases are related to more enhanced MRGTDs, although they can also be secondarily affected by Kelvin waves. For those MRGTD-enhanced MJO events, MJO convective initiation is primarily triggered by low-level MRGTD circulations that develop via the enhancement of downward energy dispersion in accordance with upper-tropospheric baroclinic conversion. This is supported by the modulation of MRGTD structure associated with zonal wave contraction due to upper-tropospheric zonal convergence, and plentiful moisture advected into the western IO. Following this MRGTD-induced MJO triggering and midtropospheric premoistening in the IO contributed by MRGTD shallow circulations as well as intraseasonal winds during the MJO-suppressed phase, low-level MRGTD winds with eastward group velocity successively trigger convection to the east, which helps MJO convective propagation over the IO. The interannual atmospheric variability may affect whether the presented MRGTD-related processes are effective or not.
Smoke with Induced Rotation and Lofting (SWIRL) in the Stratosphere
Authors: Allen Douglas R. , Fromm Michael D. , Kablick III George P. , Nedoluha Gerald E. 
Published: October 2020

AbstractThe Australian bushfires of 2019/20 produced an unusually large number of pyrocumulonimbus (pyroCb) that injected huge amounts of smoke into the lower stratosphere. The pyroCbs from 29 December 2019 to 4 January 2020 were particularly intense, producing hemispheric-wide aerosol that persisted for months. One plume from this so-called Australian New Year (ANY) event evolved into a stratospheric aerosol mass ~1000 km across and several kilometers thick. This plume initially moved eastward toward South America in January, then reversed course and moved westward passing south of Australia in February and eventually reached South Africa in early March. The peculiar motion was related to the steady rise in plume potential temperature of ~8 K day−1 in January and ~6 K day−1 in February, due to local heating by smoke absorption of solar radiation. This heating resulted in a vertical temperature anomaly dipole, a positive potential vorticity (PV) anomaly, and anticyclonic circulation. We call this dynamical component of the smoke plume “smoke with induced rotation and lofting” (SWIRL). This study uses Navy Global Environmental Model (NAVGEM) analyses to detail the SWIRL structure over 2 months. The main diagnostic tool is an anticyclone edge calculation based on the scalar Q diagnostic. This provides the framework for calculating the time evolution of various SWIRL properties: PV anomaly, streamfunction, horizontal size, vertical thickness, flow speed, and tilt. In addition, we examine the temperature anomaly dipole, the SWIRL interaction with the large-scale wind shear, and the ozone anomaly associated with lofting of air from the lower to the middle stratosphere.
The Influence of Turbulence Memory on Idealized Tornado Simulations
Authors: Wang Aaron , Pan Ying , Markowski Paul M. 
Published: October 2020

AbstractSurface friction contributes to tornado formation and maintenance by enhancing the convergence of angular momentum. The traditional lower boundary condition in atmospheric models typically assumes an instant equilibrium between the unresolved stress and the resolved shear. This assumption ignores the physics that turbulent motions are generated and dissipated at finite rates—in effect, turbulence has a memory through its lifetime. In this work, a modified lower boundary condition is proposed to account for the effect of turbulence memory. Specifically, when an air parcel moves along a curved trajectory, a normal surface-shear-stress component arises owing to turbulence memory. In the accompanying large-eddy simulation (LES) of idealized tornadoes, the normal surface-shear-stress component is a source of additional dynamic instability, which provides an extra pathway for the development of turbulent motions. The influence of turbulence memory on the intensity of quasi-steady-state tornadoes remains negligible as long as assumptions employed by the modified lower boundary condition hold over a relatively large fraction of the flow region of interest. However, tornadoes in a transient state may be especially sensitive to turbulence memory.Significance StatementFriction between the wind and the ground can influence atmospheric phenomena in important ways. For example, surface friction can be a significant source of rotation in some thunderstorms, and it can also help to intensify rotation when rotation is already present. Unfortunately, the representation of friction’s effects in atmospheric simulations is especially error-prone in phenomena characterized by rapid temporal evolution or strong spatial variations. Our work explores a new framework for representing friction to include the effect of the so-called turbulence memory. The approach is tested in idealized tornado simulations, but it may be applied to a wide range of atmospheric vortices.
Can Organizations Leverage Employer Branding to Gain Employee Engagement and Reduce Turnover Intention? An Empirical Study with Organizational Trust as a Mediator
Authors: Yadav Lalit Kumar , Kumar Ashish , Mishra Shubhra 
Published: October 2020

Employee engagement (EE) and turnover intention (TI) are significant goals to pursue for all vibrant organizations. To pursue these goals, organizations need to differentiate itself from other organizations. One important concept that leads organization to a different pedestal is employer branding (EB). This work studies the influence of EB on EE and TI. It also attempts to explain the mechanism through which EB impacts these two variables. This is done by incorporating organizational trust (OT) as a mediating variable. he sample size of the study is 308, and the respondents were from various organizations spread across India. The statistical tools to get the result were correlation and hierarchical regression in SPSS. The study finds that EB is positively related to EE and negatively aligned with TI. OT partially mediated and fully mediated the relation with EE and turnover intention, respectively. After discussing the result, implications, both practical and theoretical, are laid out. Limitations and further scope of research are discussed in the end.
The cost-effectiveness of azithromycin in reducing exacerbations in uncontrolled asthma
Authors: O'Neill Ciaran , Gibson Peter G. , Heaney Liam G. , Upham John W. , Yang Ian A. , Reynolds Paul N. , Hodge Sandra , Jenkins Christine R. , Peters Matthew , Marks Guy B. , James Alan L. , Simpson Jodie L. 
Published: October 2020

Add-on azithromycin (AZM) results in a significant reduction in exacerbations among adults with persistent uncontrolled asthma. The aim of this study was to assess the cost-effectiveness of add-on AZM in terms of healthcare and societal costs.The AMAZES trial randomly assigned 420 participants to AZM or placebo. Healthcare use and asthma exacerbations were measured during the treatment period. Healthcare use included all prescribed medicine and healthcare contacts. Costs of antimicrobial resistance (AMR) were estimated based on overall consumption and published estimates of costs. The value of an avoided exacerbation was based on published references. Differences in cost between the two groups were related to differences in exacerbations in a series of net monetary benefit estimates. Societal costs included lost productivity, over the counter medicines, steroid induced morbidity and AMR costs.Add-on AZM resulted in a reduction in healthcare costs (mean (95% CI)) including nights in hospital (AUD 433.70 (AUD 48.59–818.81) or EUR 260.22 (EUR 29.15–491.29)), unplanned healthcare visits (AUD 20.25 (AUD 5.23–35.27) or EUR 12.15 (EUR 3.14–21.16)), antibiotic costs (AUD 14.88 (AUD 7.55–22.21) or EUR 8.93 (EUR 4.53–13.33)) and oral corticosteroid costs (AUD 4.73 (AUD 0.82–8.64) or EUR 2.84 (EUR 0.49–5.18)); all p<0.05. Overall healthcare and societal costs were lower (AUD 77.30 (EUR 46.38) and AUD 256.22 (EUR 153.73) respectively) albeit not statistically significant. The net monetary benefit of add-on AZM was estimated to be AUD 2072.30 (95% CI AUD 1348.55–2805.23) or (EUR 1243.38 (EUR 809.13–1683.14) assuming a willingness to pay per exacerbation avoided of AUD 2651 (EUR 1590.60). Irrespective of the sensitivity analysis applied, the net monetary benefit for total, moderate and severe exacerbations remained positive and significant.Add-on AZM therapy in poorly controlled asthma was a cost-effective therapy. Costs associated with AMR did not influence estimated cost-effectiveness.
MapGL: inferring evolutionary gain and loss of short genomic sequence features by phylogenetic maximum parsimony
Authors: Diehl Adam G. , Boyle Alan P. 
Published: October 2020

Abstract
Background
Comparative genomics studies are growing in number partly because of their unique ability to provide insight into shared and divergent biology between species. Of particular interest is the use of phylogenetic methods to infer the evolutionary history of cis-regulatory sequence features, which contribute strongly to phenotypic divergence and are frequently gained and lost in eutherian genomes. Understanding the mechanisms by which cis-regulatory element turnover generate emergent phenotypes is crucial to our understanding of adaptive evolution. Ancestral reconstruction methods can place species-specific cis-regulatory features in their evolutionary context, thus increasing our understanding of the process of regulatory sequence turnover. However, applying these methods to gain and loss of cis-regulatory features historically required complex workflows, preventing widespread adoption by the broad scientific community.

Results
MapGL simplifies phylogenetic inference of the evolutionary history of short genomic sequence features by combining the necessary steps into a single piece of software with a simple set of inputs and outputs. We show that MapGL can reliably disambiguate the mechanisms underlying differential regulatory sequence content across a broad range of phylogenetic topologies and evolutionary distances. Thus, MapGL provides the necessary context to evaluate how genomic sequence gain and loss contribute to species-specific divergence.

Conclusions
MapGL makes phylogenetic inference of species-specific sequence gain and loss easy for both expert and non-expert users, making it a powerful tool for gaining novel insights into genome evolution.
Genome-wide identification and expression analysis of AP2/ERF transcription factors in sugarcane (Saccharum spontaneum L.)
Authors: Li Peiting , Chai Zhe , Lin Pingping , Huang Chaohua , Huang Guoqiang , Xu Liangnian , Deng Zuhu , Zhang Muqing , Zhang Yu , Zhao Xinwang 
Published: October 2020

Abstract
Background
APETALA2/ETHYLENE RESPONSIVE FACTOR (AP2/ERF) transcription factors play essential roles in plant growth, development, metabolism, and responses to biotic and abiotic stresses. However, few studies concerning AP2/ERF genes in sugarcane which are the most critical sugar and energy crops worldwide.

Results
A total of 218 AP2/ERF genes were identified in the Saccharum spontaneum genome. Phylogenetic analysis showed that these genes could be divided into four groups, including 43 AP2s, 160 ERFs and Dehydration-responsive element-binding (DREB) factors, 11 ABI3/VPs (RAV), and four Soloist genes. These genes were unevenly distributed on 32 chromosomes. The structural analysis of SsAP2/ERF genes showed that 91 SsAP2/ERFs lacked introns. Sugarcane and sorghum had a collinear relationship between 168 SsAP2/ERF genes and sorghum AP2/ERF genes that reflected their similarity. Multiple cis-regulatory elements (CREs) present in the SsAP2/ERF promoter were related to abiotic stresses, suggesting that SsAP2/ERF activity could contribute to sugarcane adaptation to environmental changes. The tissue-specific analysis showed spatiotemporal expression of SsAP2/ERF in the stems and leaves of sugarcane at different development stages. In ten sugarcane samples, 39 SsAP2/ERFs were not expressed, whereas 58 SsAP2/ERFs were expressed in all samples. Quantitative PCR experiments showed that SsERF52 expression was up-regulated under salt stress, but suppressed under dehydration stress. SsSoloist4 had the most considerable upregulation in response to treatment with the exogenous hormones ABA and GA. Within 3 h of ABA or PEG6000 treatment, SsSoloist4 expression was up-regulated, indicating that this gene could play a role in the responses to ABA and GA-associated dehydration stress. Analysis of AP2/ERF gene expression patterns under different treatments indicated that SsAP2/ERF genes played an essential role in dehydration and salt stress responses of S. spontaneum.

Conclusions
In this study, a total of 218 members of the AP2 / ERF superfamily were identified in sugarcane, and their genetic structure, evolution characteristics, and expression patterns were studied and analyzed. The results of this study provide a foundation for future analyses to elucidate the importance of AP2/ERF transcription factors in the function and molecular breeding of sugarcane.
Transcriptional landscape of the embryonic chicken Müllerian duct
Authors: Roly Zahida Yesmin , Godini Rasoul , Estermann Martin A. , Major Andrew T. , Pocock Roger , Smith Craig A. 
Published: October 2020

AbstractBackgroundMüllerian ducts are paired embryonic tubes that give rise to the female reproductive tract in vertebrates. Many disorders of female reproduction can be attributed to anomalies of Müllerian duct development. However, the molecular genetics of Müllerian duct formation is poorly understood and most disorders of duct development have unknown etiology. In this study, we describe for the first time the transcriptional landscape of the embryonic Müllerian duct, using the chicken embryo as a model system. RNA sequencing was conducted at 1 day intervals during duct formation to identify developmentally-regulated genes, validated by in situ hybridization.ResultsThis analysis detected hundreds of genes specifically up-regulated during duct morphogenesis. Gene ontology and pathway analysis revealed enrichment for developmental pathways associated with cell adhesion, cell migration and proliferation, ERK and WNT signaling, and, interestingly, axonal guidance. The latter included factors linked to neuronal cell migration or axonal outgrowth, such as Ephrin B2, netrin receptor, SLIT1 and class A semaphorins. A number of transcriptional modules were identified that centred around key hub genes specifying matrix-associated signaling factors;SPOCK1, HTRA3andADGRD1. Several novel regulators of the WNT and TFG-β signaling pathway were identified in Müllerian ducts, includingAPCDD1andDKK1,BMP3andTGFBI. A number of novel transcription factors were also identified, includingOSR1, FOXE1, PRICKLE1, TSHZ3andSMARCA2. In addition, over 100 long non-coding RNAs (lncRNAs) were expressed during duct formation.ConclusionsThis study provides a rich resource of new candidate genes for Müllerian duct development and its disorders. It also sheds light on the molecular pathways engaged during tubulogenesis, a fundamental process in embryonic development.
Integrated sRNAome and RNA-Seq analysis reveals miRNA effects on betalain biosynthesis in pitaya
Authors: Chen Canbin , Xie Fangfang , Hua Qingzhu , Tel-Zur Noemi , Zhang Lulu , Zhang Zhike , Zhang Rong , Zhao Jietang , Hu Guibing , Qin Yonghua 
Published: October 2020

Abstract
Background
MicroRNAs (miRNAs) and their regulatory functions in anthocyanin, carotenoid, and chlorophyll accumulation have been extensively characterized in many plant species. However, the miRNA regulatory mechanism in betalain biosynthesis remains mostly unknown.

Results
In this study, 126 conserved miRNAs and 41 novel miRNAs were first isolated from Hylocereus monacanthus, among which 95 conserved miRNAs belonged to 53 miRNA families. Thirty-four candidate miRNAs related to betalain biosynthesis were differentially expressed. The expression patterns of those differential expressed miRNAs were analyzed in various pitaya tissues by RT-qPCR. A significantly negative correlation was detected between the expression levels of half those miRNAs and corresponding target genes. Target genes of miRNAs i.e. Hmo-miR157b-HmSPL6-like, Hmo-miR160a-Hpcyt P450-like3, Hmo-miR6020-HmCYP71A8-like, Hmo-novel-2-HmCYP83B1-like, Hmo-novel-15-HmTPST-like, Hmo-miR828a-HmTT2-like, Hmo-miR858-HmMYB12-like, Hmo-miR858-HmMYBC1-like and Hmo-miR858-HmMYB2-like were verified by 5′RACE and transient expression system in tobacco.

Conclusions
Hmo-miR157b, Hmo-miR160a, Hmo-miR6020 Hmo-novel-2, Hmo-novel-15, Hmo-miR828a and Hmo-miR858 play important roles in pitaya fruit coloration and betalain accumulation. Our findings provide new insights into the roles of miRNAs and their target genes of regulatory functions involved in betalain biosynthesis of pitaya.
Spatial and temporal patterns of Ross River virus in south east Queensland, Australia: identification of hot spots at the rural-urban interface
Authors: Murphy Amanda K. , Clennon Julie A. , Vazquez-Prokopec Gonzalo , Jansen Cassie C. , Frentiu Francesca D. , Hafner Louise M. , Hu Wenbiao , Devine Gregor J. 
Published: October 2020

Abstract
Background
Ross River virus (RRV) is responsible for the most common vector-borne disease of humans reported in Australia. The virus circulates in enzootic cycles between multiple species of mosquitoes, wildlife reservoir hosts and humans. Public health concern about RRV is increasing due to rising incidence rates in Australian urban centres, along with increased circulation in Pacific Island countries. Australia experienced its largest recorded outbreak of 9544 cases in 2015, with the majority reported from south east Queensland (SEQ). This study examined potential links between disease patterns and transmission pathways of RRV.

Methods
The spatial and temporal distribution of notified RRV cases, and associated epidemiological features in SEQ, were analysed for the period 2001–2016. This included fine-scale analysis of disease patterns across the suburbs of the capital city of Brisbane, and those of 8 adjacent Local Government Areas, and host spot analyses to identify locations with significantly high incidence.

Results
The mean annual incidence rate for the region was 41/100,000 with a consistent seasonal peak in cases between February and May. The highest RRV incidence was in adults aged from 30 to 64 years (mean incidence rate: 59/100,000), and females had higher incidence rates than males (mean incidence rates: 44/100,000 and 34/100,000, respectively). Spatial patterns of disease were heterogeneous between years, and there was a wide distribution of disease across both urban and rural areas of SEQ. Overall, the highest incidence rates were reported from predominantly rural suburbs to the north of Brisbane City, with significant hot spots located in peri-urban suburbs where residential, agricultural and conserved natural land use types intersect.

Conclusions
Although RRV is endemic across all of SEQ, transmission is most concentrated in areas where urban and peri-urban environments intersect. The drivers of RRV transmission across rural-urban landscapes should be prioritised for further investigation, including identification of specific vectors and hosts that mediate human spillover.
The psychosocial determinants of quality of life in breast cancer survivors: a scoping review
Authors: Culbertson Michael G. , Bennett Kathleen , Kelly Catherine M. , Sharp Linda , Cahir Caitriona 
Published: October 2020

Abstract
Background
Breast cancer care today involves state-of-the-art biomedical treatment but can fail to address the broader psychosocial and quality-of-life (QoL) issues associated with the transition to breast cancer survivorship. This scoping review examines the evidence on the influence of psychosocial determinants on QoL in breast cancer survivors.

Methods
Scoping review methodology was used to: (1) identify the research question(s); (2) identify relevant studies; (3) undertake study selection; (4) extract data; (5) collate, summarise and report the results.

Results
A total of 33 studies met the inclusion criteria. The majority of studies were conducted in the US (n = 22, 67%) and were mainly cross-sectional (n = 26, 79%). Sixteen psychosocial determinants of QoL were identified. Social support (n = 14, 42%), depression (n = 7, 21%) and future appraisal and perspective (n = 7, 21%) were the most frequently investigated determinants. Twelve different QoL measures were used. A range of different measurement tools were also used per psychosocial determinant (weighted average = 6). The 14 studies that measured the influence of social support on QoL employed 10 different measures of social support and 7 different measures of QoL. In general, across all 33 studies, a higher level of a positive influence and a lower level of a negative influence of a psychosocial determinant was associated with a better QoL e.g. higher social support and lower levels of depression were associated with a higher/better QoL. For some determinants such as spirituality and coping skills the influence on QoL varied, but these determinants were less commonly investigated.

Conclusion
Consensus around measures of QoL and psychological determinants would be valuable and would enable research to determine the influence of psychosocial determinants on QoL adequately. Research in other healthcare settings beyond the US is required, in order to understand the influence of organisation and follow-up clinical and supportive care on psychosocial determinants and QoL and to improve the quality of care in breast cancer survivors.