bluuebunny/crossref_metadata_2025_split huggingface dataset:
Using Wireline Logging and Thin Sections to Identify Igneous Contact Metamorphism and Hydrothermal Influence on Presalt Limestone Reservoirs in Libra Block, Santos Basin
Authors: Ren Kangxu , Oliveira Maria José Resende , Zhao Junfeng , Zhao Jian , Oliveira Leonardo Costa , Rancan Cristiano Camelo , Carmo Isabela Oliveira , Deng Qicai 
Published: October 2019

A methodology to confirm the direct contact metamorphism of intrusive rocks and related hydrothermal modification on presalt limestone reservoirs, in Libra Block of Santos Basin, is presented. The approach is based on systemic study of cores, sidewall cores, traditional wireline logging, ECS and NMR of boreholes. Type and characteristics of metamorphism in contact aureoles between intrusive bodies and limestones are confirmed. NMR was used to evaluate porosity change of limestone. Thin sections and ECS were used to identify the precipitant types and to confirm the modification of hydrothermal fluids influences on surrounding limestones.
Intrusive rocks are observed in several wells of presalt layers in Libra Block and show contact metamorphic aureoles in the surrounding limestones, characterized by a significant change on the limestone mineral size, rock color, and reservoir pore after marbleization. By combining conventional logging and NMR logging, the thicknesses of contact metamorphic aureoles are determined in multiple wells. They conform to a binomial regression relation with the thicknesses intrusive bodies. The NMR data reveals that igneous contact metamorphism exerts negative influence on pores development for carbonate reservoirs. Reservoir physical properties decrease sharply after thermal baking, generally by 4 to 8 times. The study also confirms that related hydrothermal fluids pervasively develop and have multi-types of influences on limestones, mainly due to silicification, dolomitization and calcification. They mainly brought minerals precipitation in fractures and pores that made limestone reservoir tight. In Libra presalt layers, hydrothermal modification related to intrusive bodies mainly developed at the middle-upper intervals of BVE Fm.
Combined with wells correlation sections and seismic data, a state-of-the-art model of igneous contact metamorphism and hydrothermal modification on limestone reservoirs has been developed in this paper. It can help to quantitatively predict the thickness of contact metamorphism aureole around intrusive rocks. The study also clarifies the understanding of the type of hydrothermal effects on the reservoir porosity and how to map the spatial distribution of hydrothermal deposition in the reservoir.
UBASH3A Regulates the Synthesis and Dynamics of TCR–CD3 Complexes
Authors: Ge Yan , Paisie Taylor K. , Chen Sixue , Concannon Patrick 
Published: October 2019

Abstract
The TCR–CD3 complex is a multicomponent membrane receptor, the expression of which is tightly regulated in thymocytes, as well as in mature T cells both at steady state and upon stimulation. In this study, we report novel roles for UBASH3A in TCR–CD3 synthesis and turnover. UBASH3A is a negative regulator of T cell function and plays a broad role in autoimmunity. We show that modulation of UBASH3A levels in unstimulated Jurkat cells leads to altered amounts of total cellular CD3 chains and of cell-surface TCR–CD3 complexes; in contrast, UBASH3A does not affect the level of cell-surface CD28, an important T cell costimulatory receptor. Upon TCR engagement, UBASH3A enhances the downmodulation of cell-surface TCR–CD3. Mass spectrometry and protein–protein interaction studies uncover novel associations between UBASH3A and components of several cellular pathways involved in the regulation of TCR–CD3 turnover and dynamics, including endoplasmic reticulum–associated protein degradation, cell motility, endocytosis, and endocytic recycling of membrane receptors. Finally, we demonstrate that the SH3 domain of UBASH3A mediates its binding to CBL-B, an E3 ubiquitin ligase that negatively regulates CD28-mediated signaling and, hence, T cell activation. In summary, this study provides new mechanistic insights into how UBASH3A regulates T cell activation and contributes to autoimmunity. The interaction between UBASH3A and CBL-B may synergistically inhibit T cell function and affect risk for type 1 diabetes, as both genes have been shown to be associated with this autoimmune disease.
Downregulation of MHC Class II by Ubiquitination Is Required for the Migration of CD206+ Dendritic Cells to Skin-Draining Lymph Nodes
Authors: Majdoubi Abdelilah , Lee Jun Seong , Balood Mohammad , Sabourin Antoine , DeMontigny Auriane , Kishta Osama A. , Moulefera Mohamed Abdelwafi , Galbas Tristan , Yun Tae Jin , Talbot Sébastien , Ishido Satoshi , Cheong Cheolho , Thibodeau Jacques 
Published: October 2019

AbstractDendritic cells (DCs) are critical players in skin homeostasis. A subset of mannose receptor (CD206)–expressing monocyte-derived DCs was found in skin, and their migratory counterpart is present in skin-draining lymph nodes (sdLNs). Skin CD206+ DCs were shown to upregulate MHC class II (MHCII) progressively, raising the question of whether this feature affects their biology. In this study, we assessed the role of MHCII regulation in the development and migration of these cells in mouse models expressing differential MHCII levels. Using CD206 as a surrogate marker, we found that skin CD206+ DCs develop in an MHCII-independent manner. However, their migration to sdLNs was affected by overexpression rather than absence or lower expression of MHCII. Accordingly, B16 tumor growth was exacerbated in mice overexpressing MHCII in the absence of ubiquitination. Mechanistically, CD206+ DCs from these mice showed decreased IRF4 and CCR7 expression. LPS, which is known to promote monocyte-derived DC recruitment to sdLNs, partially improved these defects. However, GM-CSF delivery restored CD206+ DC migration by promoting IRF4 expression. Collectively, these data show that MHCII downregulation is crucial for IRF4-dependent migration of CD206+ DCs to sdLNs in health and disease.
Racialized Osteology and Athletic Aptitude, or “Black” Bones as Red Herrings
Authors: Schultz Jaime 
Published: October 2019

AbstractResearch about “black” bones generated within the fields of craniometry, forensic anthropology, and sport science affects ideas about the suitability of “black” bodies for collision and aquatic sports. The belief that people of African descent have thicker, denser bones presupposes an attribute that allegedly guards against fracture but impedes buoyancy: “black” bones are less likely to break and more likely to sink. The mythology of strong black bones stokes ideas about black “hardiness, “ an imaginary that at once dehumanizes groups and individuals as it holds them up as superhuman beings. Ultimately, the unqualified and uncritical ossification of racial categories in sport studies and allied fields perpetuate pernicious racial stereotypes.
Toekomstverkenningen in het beleidsonderzoek
Authors: Benschop Hans Peter , Veenma Klaas 
Published: October 2019

Toekomstverkenningen in het beleidsonderzoek
Wij betogen dat er een aantal goede redenen is om rekening te houden met onzekerheid in het beleidsonderzoek. Centraal staat daarbij het uitgangspunt dat de toekomst open is, maar niet leeg. In beleidsonderzoek is het zaak om die open, maar niet lege toekomst voor beslissers in beeld te brengen. Door met verschillende mogelijke toekomstbeelden te werken kan het beleid robuuster worden vormgegeven. Is dit beleid nog steeds een goede keuze als zaken in de toekomst toch anders lopen dan we nu denken? Door onzekerheden zichtbaar te maken wordt de bestuurder bewust gemaakt van de keuzes die hij maakt, worden nieuwe alternatieven zichtbaar en daarmee zijn geest 'opgerekt'. Ook uit maatschappelijk oogpunt is het nadrukkelijker rekening houden met onzekerheid over de toekomst wenselijk: mogelijke overinvesteringen kunnen worden voorkomen. We presenteren niet alleen het 'klassieke' scenariodenken, maar ook relatief nieuwe ontwikkelingen als radicale onzekerheid ('black swans'), normatieve scenario's en adaptief beleid ('adaptive policymaking').
Techniek: goed voor u!
Authors: de Koning Jaap 
Published: October 2019

Techniek: goed voor u!
Het aandeel van de industrie in de Nederlandse economie neemt structureel af. Deze ontwikkeling wordt versterkt door een tekort aan binnenlands aanbod van technici: de belangstelling voor technische opleidingen is sterk teruggelopen. In de toekomst dreigt het tekort aan technici verder op te lopen, waardoor de positie van de Nederlandse industrie verder onder druk komt te staan. Voor een open economie als die van Nederland is de industrie, die het overgrote deel van de export voor zijn rekening neemt, echter van grote betekenis. Ook biedt de industrie relatief goed betaalde banen voor werknemers die anders aangewezen zouden zijn op laag betaalde arbeid of geen baan zouden kunnen krijgen. Om de dalende trend te keren moeten meer jongeren geïnteresseerd worden in een technische opleiding. In het artikel komt uitvoerig aan de orde hoe dat kan.
Modeling of the limiting digging force of hydraulic excavator based on resistance characteristics
Authors: REN Zhigui , WANG Junli , ZOU Zhihong , WANG Yanyan , ZHU Haojie 
Published: October 2019

Based on the resistance characteristics, a model of theoretical digging force was proposed in this paper, taking the tangential force, the normal force, and the bending moment into account simultaneously. Utilizing the relation among the normal resistance, the resistance moment, and the tangential resistance in practical digging process, three independent unknown quantities are transformed into only one variable. Afterwards, according to different digging patterns and complete machine limiting conditions, this research derived the constraint inequalities of the limiting digging force (LDF) and established the calculation models for LDF. Then, based on the value distribution laws of the digging resistance coefficient and the resistance moment coefficient, the calculation process and corresponding method of LDF under a given digging posture were obtained. Taking the digging resistance obtained by testing for 35 t hydraulic excavator with backhoe attachment as the reference, this paper compared the calculation results of the theoretical digging force for complete machine with those of the LDF model proposed in this research. The comparative results indicate that the LDF is consistent with the fact that the theoretical digging force is larger than or at least equal to the actual digging resistance. So, the LDF can exactly show the real limiting digging ability of the excavator more accurately. In this way, it can provide basis for mechanism optimization, structural strength design, trajectory planning, and control automation of the excavator.
Revision of Chondrocyclus s.l. (Mollusca: Cyclophoridae), with description of a new genus and twelve new species
Authors: Cole Mary L. 
Published: October 2019

Chondrocyclus Ancey, 1898 is a genus of nine species of African operculate land snails restricted to indigenous forest and mesic thicket. Worn specimens (i.e., without a periostracum or operculum), on which some species descriptions and records were based, appear to be indistinguishable morphologically. A comprehensive revision of Chondrocyclus s.l. is provided here based on comparative morphological examinations of the shell, protoconch, periostracum, operculum, radula and penis, and on mitochondrial genes cytochrome c oxidase subunit I and 16S rRNA. Two genus-level lineages are recognised, Chondrocyclus s.s. and Afrocyclus gen. nov. Revised species descriptions are given for seven species. Two species, C. meredithae Bruggen, 1983 and C. chirindae Bruggen, 1986 both from north of South Africa, are removed from Chondrocyclus. Twelve new species are described: C. herberti sp. nov., C. silvicolus sp. nov., C. amathole sp. nov., C. pondoensis sp. nov., C. devilliersi sp. nov., C. pulcherrimus sp. nov., C. cooperae sp. nov., C. langebergensis sp. nov., C. kevincolei sp. nov., A. oxygala gen. et sp. nov., A. potteri gen. et sp. nov. and A. bhaca gen. et sp. nov. This is the first detailed systematic revision of an Afrotropical cyclophorid group to include morphological and molecular data. This study complements research on other taxa of low-vagility forest-dwelling habitat specialists by providing comparative distribution data for an independent, widespread group. Such evidence is urgently needed for conservation of South Africa’s threatened forest biome.
An Osteopathic Physician's Approach to the Esports Athlete
Authors: Zwibel Hallie , DiFrancisco-Donoghue Joanne , DeFeo Amanda , Yao Sheldon 
Published: October 2019

Abstract
Esports is gaining acceptance in the world of professional, collegiate, and high school athletics. However, there is a lack of information for osteopathic physicians about the health concerns and appropriate treatment of esports athletes. Because of the sedentary nature of the sport and accompanying poor posture, esports athletes are likely to have musculoskeletal injuries of the neck, back, and upper extremities. Additionally, these athletes may have metabolic disturbances resulting from light-emitting diode computer monitors as well as mental health concerns regarding gaming addiction and social behavior disorders. The authors explore the osteopathic physician's role in promoting health and reducing injury in this new gaming phenomenon.
miR‐15a‐5p, miR‐15b‐5p, and miR‐16‐5p inhibit tumor progression by directly targeting MYCN in neuroblastoma
Authors: Chava Srinivas , Reynolds C. Patrick , Pathania Anup S. , Gorantla Santhi , Poluektova Larisa Y. , Coulter Don W. , Gupta Subash C. , Pandey Manoj K. , Challagundla Kishore B. 
Published: October 2019

Neuroblastoma (NB) is the most common extracranial solid malignancy in children. Despite current aggressive treatment regimens, the prognosis for high‐risk NB patients remains poor, with the survival of less than 40%. Amplification/stabilization of MYCN oncogene, in NB is associated with a high risk of recurrence. Thus, there is an urgent need for novel therapeutics. The deregulated expression of microRNA (miR) is reported in NB; nonetheless, its effect on MYCN regulation is poorly understood. First, we identified that miR‐15a‐5p, miR‐15b‐5p, and miR‐16‐5p (hereafter miR‐15a, miR‐15b or miR‐16) were down‐regulated in patient‐derived xenografts (PDX) with high MYCN expression. MiR targeting sequences on MYCN mRNA were predicted using online databases such as TargetScan and miR database. The R2 database, containing 105 NB patients, showed an inverse correlation between MYCN mRNA and deleted in lymphocytic leukemia (DLEU) 2, a host gene of miR‐15. Moreover, overexpression of miR‐15a, miR‐15b or miR‐16 significantly reduced the levels of MYCN mRNA and N‐Myc protein. Conversely, inhibiting miR dramatically enhanced MYCN mRNA and N‐Myc protein levels, as well as increasing mRNA half‐life in NB cells. By performing immunoprecipitation assays of argonaute‐2 (Ago2), a core component of the RNA‐induced silencing complex, we showed that miR‐15a, miR‐15b and miR‐16 interact with MYCN mRNA. Luciferase reporter assays showed that miR‐15a, miR‐15b and miR‐16 bind with 3’UTR of MYCN mRNA, resulting in MYCN suppression. Moreover, induced expression of miR‐15a, miR‐15b and miR‐16 significantly reduced the proliferation, migration, and invasion of NB cells. Finally, transplanting miR‐15a‐, miR‐15b‐ and miR‐16‐expressing NB cells into NSG mice repressed tumor formation and MYCN expression. These data suggest that miR‐15a, miR‐15b and miR‐16 exert a tumor‐suppressive function in NB by targeting MYCN. Therefore, these miRs could be considered as potential targets for NB treatment.
Methanothermococcus
Authors: Rother Michael , Whitman William B. 
Published: October 2019

AbstractMe.tha.no.ther.mo.coc'cus. N.L. neut. n.methanummethane; N.L. pref.methano‐, pertaining to methane; Gr. masc. adj. thermos hot; N.L. masc. n.coccus(from Gr. masc. n.kokkos, grain, seed)coccus; N.L. masc. n.Methanothermococcus, a coccus producing methane at thermophilic growth temperatures.Euryarchaeota / Methanococci / Methanococcales / Methanococcaceae / MethanothermococcusRegular to irregular cocci, 1.5 µm in diameter. Cells stain Gram‐negative and lyse rapidly in dilute solutions of sodium dodecyl sulfate. Motile, by means of a polar tuft of flagella. Obligately anaerobic. Thermophilic (temperature optimum: 60–65°C). pH optimum between 5.1 and 7.5. NaCl required for growth, optimal concentration 2–4% (w/v). Obligately methanogenic; H2and formate serve as electron donors. Acetate, methanol, and methylamines are not substrates for methanogenesis. Alcohols, including isopropanol, are not substrates for methanogenesis. Grows autotrophically in mineral medium. Organic carbon sources are not stimulatory. Nitrogen sources include ammonium, nitrate, and N2gas. Sulfur sources include sulfide, elemental sulfur, and other oxidized sulfur‐containing compounds such as thiosulfate, sulfite, and sulfate. Isolated from shallow, sandy, geothermally heated marine sediments and marine oil‐reservoir water.DNA G+C content (mol%): 31–34.Type species:Methanothermococcus thermolithotrophicus(Huber, Thomm, König, Thies and Stetter 1982) Whitman 2002, 687VP(Effective publication: Whitman 2001, 242) (basonymMethanococcus thermolithotrophicusHuber, Thomm, König, Thies and Stetter 1982, 50).
D
esulfocastanea gen. nov.
Authors: Galushko Alexander , Kuever Jan 
Published: October 2019

Abstract

De.su.fo.cas.ta'ne.a. L. pref.
de
‐ off, from; L. neut. n.
sulfur
sulfur; L. fem. n.
castanea
(from Gr. fem. n.
kastaneia
) chestnut; N.L. fem. n.
Desulfocastanea
a chestnut like sulfate‐reducing bacterium, pertaining to the bud formation on the surface.

Desulfobacterota / Desulfobulbia / Desulfobulbales / Desulfocapsaceae / Desulfocastanea gen. nov.

Oval‐ to lemon‐shaped cells, 1.3–1.8 µm × 2.2–2.8 µm. Occur singly or in pairs. Spore formation is not observed. Gram‐stain‐negative. Nonmotile. Strictly anaerobic, having a respiratory and a fermentative type of metabolism. Chemoorganoheterotroph and chemolithoautotroph, using H
2
, formate, acetate, dicarboxylic and short‐ and long‐chain fatty acids, other simple organic compounds, and various aromatic compounds as carbon sources and electron donors for anaerobic respiration. Organic substrates are completely oxidized to CO
2
via the anaerobic C
1
‐pathway (carbon monoxide dehydrogenase or Wood–Ljungdahl pathway), but intermediate formation of acetate can occur. For chemolithoautotrophic growth, H
2
can serve as the electron donor and CO
2
as the carbon source. Sulfate, sulfite, and thiosulfate serve as terminal electron acceptors and are reduced to H
2
S. In addition, nitrate can be used as terminal electron acceptor and is reduced to ammonia. Mesophilic. Optimal temperature for growth, 28°C. The optimal pH for growth is 6.9–7.1. Growth in freshwater medium with concentration of NaCl up to 5.0 g/l, and the optimal content of NaCl for growth is 1.0 g/l. Media containing a reductant and vitamins are necessary for cultivation. The genus currently accommodates a single species
Desulfocastanea catecholica
. The type strain of the genus was isolated from anoxic sediment of Delaware Bay, north‐east of Nelson, New Zealand.

DNA G + C content (mol%)
: 52.4 (
T
m
).

Type species
:
Desulfocastanea catecholica
(Szewzyk and Pfennig 1987) comb. nov. (basonym:
Desulfobacterium catecholicum
Szewzyk and Pfennig 1988, 136).
D
esulfocucumis
Authors: Watanabe Miho , Fukui Manabu , Kuever Jan 
Published: October 2019

Abstract

De.sul.fo.cu'cu.mis. L. pref.
de
from; L. neut. n.
sulfur
sulfur; L. masc. n.
cucumis
cucumber; N.L. masc. n.
Desulfocucumis
a sulfate‐reducing cucumber‐shaped bacterium.

Firmicutes / Clostridia / Clostridiales / Desulfocucumaceae / Desulfocucumis

Cells are curved rod to vibroid. Gram‐stain‐negative. Spherical spores occur at cell terminals without clear swelling sporangium. Motile with peritrichous flagella. Strictly anaerobic, having a respiratory type of metabolism. Mesophilic and neutrophilic. NaCl is not required for growth. Chemolithoautotroph or chemoorganoheterotroph using organic acids, yeast extract, and alcohols. The substrates are oxidized to CO
2
via the anaerobic C
1
‐pathway (carbon monoxide dehydrogenase or Wood–Ljungdahl pathway). Addition of yeast extract enhances the growth on acetate, but it is not required for growth. H
2
can serve as the electron donor and CO
2
as the carbon source. Sulfate, thiosulfate, and elemental sulfur are used as electron acceptors and reduced to sulfide; sulfite, nitrate, nitrite, and Fe (III) are not utilized. Grows fermentatively on organic acids but not on sugars. Desulfoviridin is absent. Major cellular fatty acids are iso‐C
15 : 0
, iso‐C
17 : 0
, and C
17 : 0
cyclopropane. Major menaquinone is MK‐7. Found in marsh soil.

DNA G + C content (mol%)
: 46.6 (LC); 49.1 (genome).

Type species
:
Desulfocucumis palustris
Watanabe, Kojima and Fukui 2017, 2681
VP
.
Salipaludibacillus
Authors: Sultanpuram Vishnuvardhan Reddy , Mothe Thirumala 
Published: October 2019

AbstractSa.li.pa.lu.di.ba.cil'lus. L. masc. n.sal salis, salt; L. fem. n.palus paludis, a swamp; L. masc. n.bacillus, a rod; N.L. masc. n.Salipaludibacillus, a rod from a salt marsh.Firmicutes / Bacilli / Bacillales / Bacillaceae / SalipaludibacillusGram‐stain‐positive, variable‐motile rods producing ellipsoidal endospores at subterminal or central position. Aerobic or facultatively anaerobic mode of growth. Positive for catalase, but nitrate reduction and oxidase activities are variable. The major isoprenoid quinone is MK‐7, sometimes with minor traces of MK‐6. The peptidoglycan is based onmeso‐diaminopimelic acid as the diagnostic diamino acid. The major polar lipids present are diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine. The major fatty acids are anteiso‐C15:0, C16:0, and iso‐C15:0. Mesophilic halophiles with a requirement of 2–5% NaCl for optimal growth. Phylogenetically, belongs to the orderBacillales, familyBacillaceae. Habitats include seasonal wetland, soil, bay, and marine wetland samples.DNA G + C content (mol%): 37.4–42.4 (HPLC and genome analysis).Type species:Salipaludibacillus aurantiacusSultanpuram and Mothe 2016, 2751VP.
Anaerocolumna
Authors: Ueki Atsuko , Kaku Nobuo , Ueki Katsuji 
Published: October 2019

AbstractAn.ae.ro.co.lum'na. Gr. prep.annot; Gr. n.aerair;anaeronot living in air; L. fem. n.columnacolumn; N.L. fem. n.Anaerocolumnaa column not living in air.Firmicutes / Clostridia / Clostridiales / Lachnospiraceae / AnaerocolumnaThe genusAnaerocolumnaincludes four species. Cells stain Gram‐positive. Motile. Strictly anaerobic, fermentative metabolism. Slightly curved to straight rods, occurring singly or in pairs. Produces terminal, spherical spores, swelling cells. Optimum temperature for growth is 30–35°C. Chemoorganotrophic, using a wide variety of compounds including sugars and organic acids as energy sources. Produces acetate, ethanol, and H2from glucose as fermentation end products. Decomposes cellulose or xylan. Catalase is absent. C16:0, C16:0dimethylacetal (DMA), C16:1ω7cDMA, or C18:1ω7cDMA are present as predominant cellular fatty acids (CFAs). The cell‐wall peptidoglycan containsmeso‐DAP as a diagnostic amino acid. Belongs to the familyLachnospiraceae. Members of the genus have been isolated mainly from methanogenic reactors or sewage sludge.DNA G + C content (mol%): 33–41 (HPLC,Tm, genome).Type species:Anaerocolumna cellulosilyticaUeki, Ohtaki, Kaku and Ueki 2016, 2942VP.