bluuebunny/crossref_metadata_2025_split huggingface dataset:
Vortex and Biogeochemical Dynamics for the Hypoxia Formation Within the Coastal Transition Zone off the Pearl River Estuary
Authors: Li Dou , Gan Jianping , Hui Rex , Liu Zhiqiang , Yu Liuqian , Lu Zhongming , Dai Minhan 
Published: August 2020

AbstractSatellite‐derived chlorophyll data, in situ measurements, and time series buoy monitoring revealed the persistent and deteriorating seasonal eutrophication and hypoxia with two distinct centers in the coastal transition zone (CTZ) between the Pearl River Estuary and the adjacent continental shelf off Hong Kong. We used a process‐oriented coupled physical‐biogeochemical numerical model to investigate the intrinsic physical and biogeochemical dynamics under the extrinsic forcing of wind, river discharge, and tides for the hypoxia formation in the CTZ. We found that the convergence induced by cyclonic vortices in the CTZ, which were formed by the buoyancy‐driven currents and wind‐driven shelf currents, created the stable water column with weak mixing and long residence time and accumulated nutrients and organic matter for the eutrophication and hypoxia development. The biophysical responses to spring‐neap cycle further demonstrated the coherent linkages of oceanic currents, vortex formation, and convergence of organic matter within the CTZ and indicated the critical role of mixing in the dissolved oxygen (DO) budgets during the spring‐neap tidal cycle. The study found that biogeochemical substances and processes are necessary conditions for eutrophication and hypoxia formation, and when these necessary conditions are combined with the favorable hydrodynamic conditions, they form a sufficient condition for eutrophication and hypoxia formation in the CTZ.
Interannual Variability of the Mid‐Atlantic Bight Cold Pool
Authors: Chen Zhuomin , Curchitser Enrique N. 
Published: August 2020

AbstractThe Mid‐Atlantic Bight (MAB) Cold Pool is a bottom‐trapped, cold (temperature below 10°C) and fresh (practical salinity below 34) water mass that is isolated from the surface by the seasonal thermocline and is located over the midshelf and outer shelf of the MAB. The interannual variability of the Cold Pool with regard to its persistence time, volume, temperature, and seasonal along‐shelf propagation is investigated based on a long‐term (1958–2007) high‐resolution regional model of the northwest Atlantic Ocean. A Cold Pool Index is defined and computed in order to quantify the strength of the Cold Pool on the interannual timescale. Anomalous strong, weak, and normal years are categorized and compared based on the Cold Pool Index. A detailed quantitative study of the volume‐averaged heat budget of the Cold Pool region (CPR) has been examined on the interannual timescale. Results suggest that the initial temperature and abnormal warming/cooling due to advection are the primary drivers in the interannual variability of the near‐bottom CPR temperature anomaly during stratified seasons. The long persistence of temperature anomalies from winter to summer in the CPR also suggests a potential for seasonal predictability.
Impacts of Protected Area Deforestation on Dry‐Season Regional Climate in the Brazilian Amazon
Authors: De Sales Fernando , Santiago Thais , Biggs Trent Wade , Mullan Katrina , Sills Erin O. , Monteverde Corrie 
Published: August 2020

AbstractRainforest in protected areas in the Brazilian Amazon is at risk due to increasing economic pressures and recent weakening of environmental agencies and legislation by the federal administration. This study examines the impacts of deforestation in protected areas on dry‐season precipitation in the Brazilian state of Rondônia located in the southwestern Brazilian Amazon. Regional‐climate model simulations indicate that clearing protected forests in Rondônia would result in substantial changes to the surface energy balance, including increased sensible and decreased latent heat flux. Consequent changes to low‐level wind circulation would enhance moisture flux convergence and convection over the newly deforested areas, leading to enhanced rainfall in those areas. However, deforestation of protected areas would decrease dry season rainfall up to 30% in the existing agricultural region, with potentially important negative impacts on agricultural production. Additionally, our results indicate that following deforestation, the newly degraded areas will experience warmer and drier afternoons that could place the remaining natural vegetation under vapor deficit stress.
Emergency response strategy for containing COVID-19 within a psychiatric specialty hospital in the epicenter of the COVID-19 epidemic in China
Authors: Ma Jun , Zhong Hao , Jiang Min , Zeng Kuan , Zhong Baoliang , Liu Lianzhong , Liu Xuebing 
Published: August 2020

AbstractCoronavirus disease 2019 (COVID-19) has been recognized as a global pandemic, and psychiatric institutions located in the epicenter of the epidemic in China are facing severe challenges in fighting the epidemic. This article presents the accumulated experience of the authors during the process of combating COVID-19 in a psychiatric hospital. The aim of this article is to provide a reference for psychiatric specialty hospitals and institutions that treat large populations of chronically ill patients in other parts of the world.
Molecular design principles of Lysine-DOPA wet adhesion
Authors: Li Yiran , Cheng Jing , Delparastan Peyman , Wang Haoqi , Sigg Severin J. , DeFrates Kelsey G. , Cao Yi , Messersmith Phillip B. 
Published: August 2020

AbstractThe mussel byssus has long been a source of inspiration for the adhesion community. Recently, adhesive synergy between flanking lysine (Lys, K) and 3,4-Dihydroxyphenylalanine (DOPA, Y) residues in the mussel foot proteins (Mfps) has been highlighted. However, the complex topological relationship of DOPA and Lys as well as the interfacial adhesive roles of other amino acids have been understudied. Herein, we study adhesion of Lys and DOPA-containing peptides to organic and inorganic substrates using single-molecule force spectroscopy (SMFS). We show that a modest increase in peptide length, from KY to (KY)3, increases adhesion strength to TiO2. Surprisingly, further increase in peptide length offers no additional benefit. Additionally, comparison of adhesion of dipeptides containing Lys and either DOPA (KY) or phenylalanine (KF) shows that DOPA is stronger and more versatile. We furthermore demonstrate that incorporating a nonadhesive spacer between (KY) repeats can mimic the hidden length in the Mfp and act as an effective strategy to dissipate energy.
Multiplexed Optical Sensors in Arrayed Islands of Cells for multimodal recordings of cellular physiology
Authors: Werley Christopher A. , Boccardo Stefano , Rigamonti Alessandra , Hansson Emil M. , Cohen Adam E. 
Published: August 2020

AbstractCells typically respond to chemical or physical perturbations via complex signaling cascades which can simultaneously affect multiple physiological parameters, such as membrane voltage, calcium, pH, and redox potential. Protein-based fluorescent sensors can report many of these parameters, but spectral overlap prevents more than ~4 modalities from being recorded in parallel. Here we introduce the technique, MOSAIC, Multiplexed Optical Sensors in Arrayed Islands of Cells, where patterning of fluorescent sensor-encoding lentiviral vectors with a microarray printer enables parallel recording of multiple modalities. We demonstrate simultaneous recordings from 20 sensors in parallel in human embryonic kidney (HEK293) cells and in human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs), and we describe responses to metabolic and pharmacological perturbations. Together, these results show that MOSAIC can provide rich multi-modal data on complex physiological responses in multiple cell types.
Low temperature synthesis of plasmonic molybdenum nitride nanosheets for surface enhanced Raman scattering
Authors: Guan Haomin , Yi Wencai , Li Tao , Li Yahui , Li Junfang , Bai Hua , Xi Guangcheng 
Published: August 2020

AbstractMolybdenum nitride (δ–MoN) is an important functional material due to its impressive catalytic, energy storage, and superconducting properties. However, the synthesis of δ–MoN usually requires extremely harsh conditions; thus, the insight into δ−MoN is far behind that of oxides and sulfides of molybdenum. Herein, we report that ultrathin δ−MoN nanosheets are prepared at 270 °C and 12 atm. WN, VN, and TiN nanosheets are also synthesized by this method. The δ−MoN nanosheets show strong surface plasmon resonance, high conductivity, excellent thermal and chemical stability as well as a high photothermal conversion efficiency of 61.1%. As a promising surface enhanced Raman scattering substrate, the δ−MoN nanosheets exhibit a 8.16 × 106 enhanced factor and a 10−10 level detection limit for polychlorophenol.
Mammalian histones facilitate antimicrobial synergy by disrupting the bacterial proton gradient and chromosome organization
Authors: Doolin Tory , Amir Henry M. , Duong Leora , Rosenzweig Rachel , Urban Lauren A. , Bosch Marta , Pol Albert , Gross Steven P. , Siryaporn Albert 
Published: August 2020

AbstractFirst proposed as antimicrobial agents, histones were later recognized for their role in condensing chromosomes. Histone antimicrobial activity has been reported in innate immune responses. However, how histones kill bacteria has remained elusive. The co-localization of histones with antimicrobial peptides (AMPs) in immune cells suggests that histones may be part of a larger antimicrobial mechanism in vivo. Here we report that histone H2A enters E. coli and S. aureus through membrane pores formed by the AMPs LL-37 and magainin-2. H2A enhances AMP-induced pores, depolarizes the bacterial membrane potential, and impairs membrane recovery. Inside the cytoplasm, H2A reorganizes bacterial chromosomal DNA and inhibits global transcription. Whereas bacteria recover from the pore-forming effects of LL-37, the concomitant effects of H2A and LL-37 are irrecoverable. Their combination constitutes a positive feedback loop that exponentially amplifies their antimicrobial activities, causing antimicrobial synergy. More generally, treatment with H2A and the pore-forming antibiotic polymyxin B completely eradicates bacterial growth.
MGMT genomic rearrangements contribute to chemotherapy resistance in gliomas
Authors: Oldrini Barbara , Vaquero-Siguero Nuria , Mu Quanhua , Kroon Paula , Zhang Ying , Galán-Ganga Marcos , Bao Zhaoshi , Wang Zheng , Liu Hanjie , Sa Jason K. , Zhao Junfei , Kim Hoon , Rodriguez-Perales Sandra , Nam Do-Hyun , Verhaak Roel G. W. , Rabadan Raul , Jiang Tao , Wang Jiguang , Squatrito Massimo 
Published: August 2020

AbstractTemozolomide (TMZ) is an oral alkylating agent used for the treatment of glioblastoma and is now becoming a chemotherapeutic option in patients diagnosed with high-risk low-grade gliomas. The O-6-methylguanine-DNA methyltransferase (MGMT) is responsible for the direct repair of the main TMZ-induced toxic DNA adduct, the O6-Methylguanine lesion. MGMT promoter hypermethylation is currently the only known biomarker for TMZ response in glioblastoma patients. Here we show that a subset of recurrent gliomas carries MGMT genomic rearrangements that lead to MGMT overexpression, independently from changes in its promoter methylation. By leveraging the CRISPR/Cas9 technology we generated some of these MGMT rearrangements in glioma cells and demonstrated that the MGMT genomic rearrangements contribute to TMZ resistance both in vitro and in vivo. Lastly, we showed that such fusions can be detected in tumor-derived exosomes and could potentially represent an early detection marker of tumor recurrence in a subset of patients treated with TMZ.
The Human LL-37(17-29) antimicrobial peptide reveals a functional supramolecular structure
Authors: Engelberg Yizhaq , Landau Meytal 
Published: August 2020

AbstractHere, we demonstrate the self-assembly of the antimicrobial human LL-37 active core (residues 17–29) into a protein fibril of densely packed helices. The surface of the fibril encompasses alternating hydrophobic and positively charged zigzagged belts, which likely underlie interactions with and subsequent disruption of negatively charged lipid bilayers, such as bacterial membranes. LL-3717–29 correspondingly forms wide, ribbon-like, thermostable fibrils in solution, which co-localize with bacterial cells. Structure-guided mutagenesis analyses supports the role of self-assembly in antibacterial activity. LL-3717–29 resembles, in sequence and in the ability to form amphipathic helical fibrils, the bacterial cytotoxic PSMα3 peptide that assembles into cross-α amyloid fibrils. This argues helical, self-assembling, basic building blocks across kingdoms of life and points to potential structural mimicry mechanisms. The findings expose a protein fibril which performs a biological activity, and offer a scaffold for functional and durable biomaterials for a wide range of medical and technological applications.
Association of low-dosage systemic corticosteroid use with disease burden in asthma
Authors: Matsunaga Kazuto , Adachi Mitsuru , Nagase Hiroyuki , Okoba Tomoko , Hayashi Nobuya , Tohda Yuji 
Published: August 2020

AbstractThere is an ongoing debate about the benefit–risk balance of systemic corticosteroids (SCS) in asthma treatment. We investigated the associations between SCS use and disease burden in a database cohort of asthmatics, categorized into SCS and non-SCS prescription at baseline and quartiles (Q) by cumulative SCS dosage. Of the 10,579 patients, the SCS cohort comprised 3103 patients (29.3%). Mean SCS dosages at baseline were 0.08, 0.29, 0.79, and 4.58 mg/day in Q1, Q2, Q3, and Q4, respectively. Similar SCS dosages were used within each quartile throughout the study period. No remarkable changes in asthma severity or control status were observed. All SCS cohorts had a higher risk of intermittent SCS exposure during the observation period. SCS use was associated with osteoporosis, diabetes, anxiety/neurosis, and depression. SCS-dependent treatment does not necessarily lead to the future improvement of asthma control; rather, it may negatively impact systemic health, even at mean dosages <5 mg/day.
Transcriptome analysis of blood and spleen in virulent and avirulent mouse malaria infection
Authors: Zhao Yuancun , Hosking Caroline , Cunningham Deirdre , Langhorne Jean , Lin Jing-wen 
Published: August 2020

AbstractMalaria is a devastating infectious disease and the immune response is complex and dynamic during a course of a malarial infection. Rodent malaria models allow detailed time-series studies of the host response in multiple organs. Here, we describe two comprehensive datasets containing host transcriptomic data from both the blood and spleen throughout an acute blood stage infection of virulent or avirulent Plasmodium chabaudi infection in C57BL/6 mice. The mRNA expression profiles were generated using Illumina BeadChip microarray. These datasets provide a groundwork for comprehensive and comparative studies on host gene expression in early, acute and recovering phases of a blood stage infection in both the blood and spleen, to explore the interaction between the two, and importantly to investigate whether these responses differ in virulent and avirulent infections.
Laser cavitation rheology for measurement of elastic moduli and failure strain within hydrogels
Authors: Luo Justin C. , Ching Herman , Wilson Bryce G. , Mohraz Ali , Botvinick Elliot L. , Venugopalan Vasan 
Published: August 2020

AbstractWe introduce laser cavitation rheology (LCR) as a minimally-invasive optical method to characterize mechanical properties within the interior of biological and synthetic aqueous soft materials at high strain-rates. We utilized time-resolved photography to measure cavitation bubble dynamics generated by the delivery of focused 500 ps duration laser radiation at λ = 532 nm within fibrin hydrogels at pulse energies ofEp = 12, 18 µJ and within polyethylene glycol (600) diacrylate (PEG (600) DA) hydrogels atEp = 2, 5, 12 µJ. Elastic moduli and failure strains of fibrin and PEG (600) DA hydrogels were calculated from these measurements by determining parameter values which provide the best fit of the measured data to a theoretical model of cavitation bubble dynamics in a Neo-Hookean viscoelastic medium subject to material failure. We demonstrate the use of this method to retrieve the local, interior elastic modulus of these hydrogels and both the radial and circumferential failure strains.
Mebendazole is unique among tubulin-active drugs in activating the MEK–ERK pathway
Authors: Andersson Claes R. , Selvin Tove , Blom Kristin , Rubin Jenny , Berglund Malin , Jarvius Malin , Lenhammar Lena , Parrow Vendela , Loskog Angelica , Fryknäs Mårten , Nygren Peter , Larsson Rolf 
Published: August 2020

AbstractWe recently showed that the anti-helminthic compound mebendazole (MBZ) has immunomodulating activity in monocyte/macrophage models and induces ERK signalling. In the present study we investigated whether MBZ induced ERK activation is shared by other tubulin binding agents (TBAs) and if it is observable also in other human cell types. Curated gene signatures for a panel of TBAs in the LINCS Connectivity Map (CMap) database showed a unique strong negative correlation of MBZ with MEK/ERK inhibitors indicating ERK activation also in non-haematological cell lines. L1000 gene expression signatures for MBZ treated THP-1 monocytes also connected negatively to MEK inhibitors. MEK/ERK phosphoprotein activity testing of a number of TBAs showed that only MBZ increased the activity in both THP-1 monocytes and PMA differentiated macrophages. Distal effects on ERK phosphorylation of the substrate P90RSK and release of IL1B followed the same pattern. The effect of MBZ on MEK/ERK phosphorylation was inhibited by RAF/MEK/ERK inhibitors in THP-1 models, CD3/IL2 stimulated PBMCs and a MAPK reporter HEK-293 cell line. MBZ was also shown to increase ERK activity in CD4+ T-cells from lupus patients with known defective ERK signalling. Given these mechanistic features MBZ is suggested suitable for treatment of diseases characterized by defective ERK signalling, notably difficult to treat autoimmune diseases.
Utility of modified vascular corrosion casting technique in the diagnosis of fetal ductus arteriosus abnormalities
Authors: Wang Yu , Zhang Jiaqi , Zeng He , Cao Haiyan , Si Ziyi , Feng Wei , Xie Mingxing 
Published: August 2020

AbstractThe anatomy of ductus arteriosus (DA) can be varied in different congenital heart defects (CHDs), and it is difficult to fully discover the DA and other associated cardiac anomalies by prenatal ultrasound. This study was aimed to use the modified vascular corrosion casting technique to prepare fetal cardiovascular casts with DA anomalies, assess the casting effectiveness in evaluating the great vessels of the fetal heart and investigate the utility of cardiovascular casting for the demonstration of fetal DA abnormalities. This retrospective study enrolled fourteen fetuses (23 to 28+2 gestational weeks) with severe CHDs diagnosed by prenatal echocardiography and casting technique from January 2013 to July 2019. The sonographic features of DAs were carefully observed and other associated cardiovascular anomalies were also evaluated during the screening. The architectures of DAs and the whole cardiovascular system were observed and analyzed, and then the cast findings were compared with prenatal ultrasonic results. In fourteen cases, 18 ductal abnormities were indicated by prenatal echocardiography in fourteen cases, while 25 were revealed by casting. Cast findings included 4 cases of ductal stenosis, 1 case of ductal dilation, 6 cases of ductal circuity, 3 cases of right-sided ductus, 5 cases of anomalous ductal connection, 1 case of bilateral ductus and 5 cases of absent ductus. Cast findings consisted with ultrasound in 10 ductal abnormalities, revealed additional 15 ductal abnormalities miss-diagnosed by sonography, and corrected 6 abnormalities misdiagnosed prenatally. Meanwhile, 3 ductal abnormalities (reversed flow) could not be demonstrated by casts but only by ultrasound. Cast models can visually display the anatomical characteristics of ductus arteriosus, and could be successfully used in the demonstration of ductus abnormalities in fetuses with severe CHDs. Comparing with ultrasound, casting technique has its own superiority in exhibiting ductus abnormalities, especially in certain types such as course, origin and absence abnormalities of ductus.
Experimental realisation of tunable ferroelectric/superconductor $$({\text {B}} {\text {T}} {\text {O}}/{\text {Y}} {\text {B}}{\text {C}} {\text {O}})_{{\text {N}}}/{\text {S}}{\text {T}}{\text {O}}$$ 1D photonic crystals in the whole visible spectrum
Authors: González Luz E. , Ordoñez John E. , Melo-Luna Carlos A. , Mendoza Evelyn , Reyes David , Zambrano Gustavo , Porras-Montenegro Nelson , Granada Juan C. , Gómez Maria E. , Reina John H. 
Published: August 2020

AbstractEmergent technologies that make use of novel materials and quantum properties of light states are at the forefront in the race for the physical implementation, encoding and transmission of information. Photonic crystals (PCs) enter this paradigm with optical materials that allow the control of light propagation and can be used for optical communication, and photonics and electronics integration, making use of materials ranging from semiconductors, to metals, metamaterials, and topological insulators, to mention but a few. Here, we show how designer superconductor materials integrated into PCs fabrication allow for an extraordinary reduction of electromagnetic waves damping, making possible their optimal propagation and tuning through the structure, below critical superconductor temperature. We experimentally demonstrate, for the first time, a successful integration of ferroelectric and superconductor materials into a one-dimensional (1D) PC composed of $$({\text {B}}{\text {T}}{\text {O}}/{\text {Y}}{\text {B}}{\text {C}} {\text {O}})_{{\text {N}}}/{\text {S}}{\text {T}}{\text {O}}$$(BTO/YBCO)N/STO bilayers that work in the whole visible spectrum, and below (and above) critical superconductor temperature $$T_C=80\, {\hbox {K}}$$TC=80K. Theoretical calculations support, for different number of bilayers N, the effectiveness of the produced 1D PCs and may pave the way for novel optoelectronics integration and information processing in the visible spectrum, while preserving their electric and optical properties.