bluuebunny/crossref_metadata_2025_split huggingface dataset:
112 Effect of an Oral Joint Supplement on Gait Kinematics and Biomarkers of Cartilage Metabolism and Inflammation in Mature Riding Horses
Authors: Much Mattea L , Leatherwood Jessica L , Martinez Rafael E , Silvers Brittany L , Basta Casey , Gray Lydia 
Published: November 2020

Abstract
Twenty stock-type horses (589 ± 126 kg BW; 13 ± 8 yr) were utilized in a completely randomized design for a 28-d trial to evaluate a joint supplement on gait kinematics, inflammation and cartilage metabolism. Horses were stratified by age, sex, body weight (BW), and initial lameness score and were randomly assigned to 1 of 2 dietary treatments consisting of either a 100 g placebo top-dressed daily to 0.6% BW (as-fed) commercial concentrate (CON; n = 10), or an oral joint supplement (SmartPak Equine LLC) containing glucosamine, chondroitin sulfate, hyaluronic acid, methylsulfonylmethane, turmeric, resveratrol, collagen, silica, and boron (TRT; n = 10). Horses had ad libitum access to coastal bermudagrass hay (Cynodon dactylon) and exercised progressively 4 d/wk, for 45 min/d. On d 13 and 27, blood was harvested following a 19.3 km exercise stressor. Every 14 d, blood was collected for plasma prostaglandin E2 (PGE2), and serum collagenase cleavage neopeptide (C2C), carboxypropeptide of type II collagen (CPII), and chondroitin sulfate 846 epitope (CS846). Gait kinematics were analyzed every 14 d (Kinovea v.0.8.15) to determine stride length (SL) and range of motion (ROM) of the knee and hock at the walk and trot. Data were analyzed using PROC MIXED of SAS. Hock ROM increased in TRT (P ≤ 0.02) at the walk and tended to increase at the trot (P = 0.09). At the walk, SL and knee ROM increased (P ≤ 0.01) in all horses. C2C and CPII increased over time (P ≤ 0.05) and no effect was observed for CS846 or PGE2 (P &gt; 0.12). In response to the exercise stressor, CPII and PGE2 decreased (P ≤ 0.05) from d 13 to 14, and CS846 and PGE2 tended to decrease (P ≤ 0.10) from d 27 to 28, independent of diet. In conclusion, hock ROM at the walk and trot was the most sensitive to TRT, but biomarker concentration of collagen metabolites and systemic inflammation was not altered in 28 d.
111 Grazing behavior of horses managed on legume-grass mixed pastures
Authors: Vasco Ana Caroline Cerqueira de Melo , Arias-Esquivel Ana Margarita , Seals Emma , da Rosa Fabiane Quevedo , Wallau Marcelo , Warren Lori K , Wickens Carissa 
Published: November 2020

Abstract
Intercropping legume into grass pastures improves pasture quality and decreases the need for nitrogen (N) fertilizer, while providing a more heterogenic grazing environment. This study aimed to evaluate the effect of rhizoma peanut (RP, Arachis glabrata) intercropped into bahiagrass (Paspalum notatum) pasture on horse grazing performance. Unfertilized bahiagrass (UNF) with no N fertilizer, bahiagrass with 120 kg N ha-1 (FER), and bahiagrass with RP and 30 kg N ha-1 (RP) pastures were evaluated using twelve mature Quarter Horses continuously stocked for 84 days in a randomized block design with two blocks. Grazing, activity and leisure behaviors were recorded every 10 minutes for 24 hours on day 35 and 70 using scan sampling. Chew (CR, chew/min) and bite (BR, bite/min) rates were counted for five consecutive 1-min intervals on days 36 and 71. Data were analyzed using a repeated measure mixed model ANOVA. Pasture did not affect (P &gt; 0.05) total time spent on grazing (13.57 ± 1.81 h, mean ± SD), leisure (9.64 ± 1.64 h), and activity (0.79 ± 0.60 h). Pasture x time of day interaction (P &lt; 0.05) was significant for grazing, leisure and activity. Horses grazing FER spent more time grazing between 1100 and 1700 h, whereas horses grazing RP spent more time grazing between 2300 and 500 h when compared to horses grazing FER. Horses grazing FER spent more time in leisure between 2300 and 700 h. No day or pasture effects were observed for BR (30.8 ± 6.3 bites/min, mean ± SD), but a pasture x day interaction was observed for CR. On day 36, CR of horses grazing FER was similar to RP (50.8 and 53.8 chews/min), but lower than RP on day 71 (41.4 and 61.3 chews/min). Intercropping RP into bahiagrass pastures does not affect total grazing, leisure and activity time of horses.
128 Growth performance, blood urea nitrogen and gut morphology of nursery pigs fed a novel, plant-based protein
Authors: Holt Jonathan P , Sundstrom Ylva , Hash Sara 
Published: November 2020

Abstract
This experiment was conducted to determine a recommended inclusion level of a novel protein source, MrFeed® Pro50, for nursery pigs. 192 pigs (BW 6.41 kg ± 0.11) were blocked by weight and placed four pigs per pen for 35 d. Dietary treatments included a commercial diet with antibiotics (AB) or diets containing 0 (CON), 5%, 10%, or 15% Pro50, fed in three phases. Pigs and feeders were weighed every 7 d. On d 0, 14 and 35, blood samples were collected via vena cava puncture. On d 35, ileum samples were collected from 48 pigs. From d 0 to d 7, ADG was greater (P &lt; 0.001) for pigs fed AB (0.15 kg/d) compared to pigs fed other treatments (: 0.09 kg, 5%: 0.09 kg/d, 10%: 0.1 kg/d, 15%: 0.09 kg/d). This is due to a greater (P = 0.002) ADFI for pigs fed AB (0.31 kg/d) compared to CON (0.26 kg/d), 5% (0.25 kg/d), 10% (0.27 kg/d) and 15% (0.27 kg/d) during the same time period. From d 28 to d 35, ADG for pigs fed AB (0.67 kg/d) or CON (0.69 kg/d) was greater than pigs fed 10% (0.56 kg/d) or 15% (0.57 kg/d), with 5% being intermediate (0.65 kg/d). At d 35, AB fed pigs (23.35 kg) weighed more than all other treatments (CON: 21.4 kg, 5%: 21.0 kg, 10%: 19.4 kg, 15%: 18.6 kg; P = 0.002). On d 35, BUN was greater (P = 0.005) for CON (14.5 mg/dL) and AB (16.5 mg/dL) fed pigs compared to 5%, 10% and 15% fed pigs (5.66 mg/dL, 6.33 mg/dL, 7.75 mg/dL, respectively). No differences (P &gt; 0.05) in villi height or crypt depth were observed. The optimal inclusion level of MrFeed® Pro50 is 5% for nursery pig diets.
146 Prematurity Blunts the Protein Synthetic Response of Skeletal Muscle to Insulin and Amino Acids
Authors: Davis Teresa A , Rudar Marko , Naberhuis Jane , Suryawan Agus , Fiorotto Marta 
Published: November 2020

Abstract
Livestock animals are important dual-purpose models that benefit both agricultural and biomedical research. The neonatal pig is an appropriate model for the human infant to assess long-term effects of early life nutrition on growth and metabolic outcomes. Previously we have demonstrated that prematurity blunts the feeding-induced stimulation of translation initiation and protein synthesis in skeletal muscle of neonatal pigs. The objective of this study was to determine whether reduced sensitivity to insulin and/or amino acids drives this blunted response. Pigs were delivered by caesarean section at preterm (PT, 103 d gestation) or at term (T, 112 d gestation) and fed parenterally for 4 d. On day 4, pigs were subject to euinsulinemic-euaminoacidemic-euglycemic (FAST), hyperinsulinemic-euaminoacidemic-euglycemic (INS), or euinsulinemic-hyperaminoacidemic-euglycemic (AA) clamps for 120 min, yielding six treatments: PT-FAST (n = 7), PT-INS (n = 9), PT-AA (n = 9), T-FAST (n = 8), T-INS (n = 9), and T-AA (n = 9). A flooding dose of L-[4-3H]Phe was injected into pigs 30 min before euthanasia. Birth weight and relative body weight gain were lower in PT than T pigs (P &lt; 0.001). Plasma insulin concentration was increased from ~3 to ~100 µU/mL in INS compared to FAST and AA pigs (P &lt; 0.001); plasma BCAA concentration was increased from ~250 to ~1,000 µmol/L in AA compared to FAST and INS pigs (P &lt; 0.001). Despite achieving similar insulin and amino acid levels, longissimus dorsi AKT phosphorylation, mechanistic target of rapamycin (mTOR)·Rheb abundance, mTOR activation, and protein synthesis were lower in PT-INS than T-INS pigs (Table 1). Although amino-acid induced dissociation of Sestrin2 from GATOR2 was not affected by prematurity, mTOR·RagA abundance, mTOR·RagC abundance, mTOR activation, and protein synthesis were lower in PT-AA than T-AA pigs. The impaired capacity of premature skeletal muscle to respond to insulin or amino acids and promote protein synthesis likely contributes to reduced lean mass accretion. Research was supported by NIH and USDA.
144 Proof of concept for bilateral twin pregnancies in Trio allele carriers
Authors: Madureira Guilherme , Gomez-León Victor , Grillo Gustavo Fernandes , Andrade João Paulo Nascimento , Lett Beth , Moghbeli Sadrollah Molaei , Wiltbank Milo C , Kirkpatrick Brian W 
Published: November 2020

Abstract
Bovine twin birth is associated with detriments including increased embryo/fetal losses, malpresentation and dystocia. Incidence of these is lessened in bilateral versus unilateral twin pregnancy. This study was undertaken to assess use of follicular ablation by aspiration to create bilateral twin pregnancies in females with genetic potential for ~3.5 ovulations per cycle (Trio allele carriers). In Experiment 1, carriers (n = 30) and non-carriers (n = 10) were synchronized for ovulation and timed artificial insemination (TAI). Follicles (&gt; 5 mm) in excess of one per ovary were aspirated ~16h preceding TAI. Follicle count for females with follicles on only one ovary was reduced to two. Blood was sampled 2 weeks post-TAI to assess progesterone (P4) concentrations; fetal count was determined by ultrasound 6 weeks post-TAI. P4 concentration post-TAI was significantly (P &lt; 0.001) associated with both genotype and subsequent pregnancy status (pregnant non-carriers, 7.06 ± 0.68 ng/mL; pregnant carriers, 5.54 ± 0.55 ng/mL; non-pregnant non-carriers, 5.22 ± 1.05 ng/mL; non-pregnant carriers, 3.13 ± 0.42 ng/mL). Experiment 2 was undertaken to offset negative effects of follicular aspiration on subsequent P4 concentration observed in Experiment 1. Carriers (n = 38) and non-carriers (n = 32) were submitted to fixed-TAI and follicle ablation as described. Additionally, accessory corpora lutea were induced by administration of hCG (carriers) at d6 post-TAI. Consequently, P4 concentration post-TAI was significantly (P &lt; 0.05) associated with subsequent pregnancy status (pregnant, 8.48 ± 0.61 ng/mL; non-pregnant, 6.70 ± 0.63 ng/mL) but not with genotype (carrier, 8.01 ± 0.59 ng/mL; non-carrier, 7.17 ± 0.64 ng/mL). Fetus number was greater in carriers (Exp1, 1.64 ± 0.81; Exp2, 1.45 ± 0.09) versus non-carriers (1.00 ± 0.00, both Experiments). Singles, twins, and triplets occurred in carriers in Experiment 1, whereas multiples in Experiment 2 were limited to twins. Genotype effects on pregnancy rate were not significant (P &gt; 0.10) in either experiment. Results suggest follicular ablation to create bilateral twin pregnancies in Trio carriers is feasible but requires induction of accessory corpora lutea to offset the negative effects of follicular aspiration on subsequent P4 concentration.
374 Early embryonic loss is an important mechanism for maximizing litter size in the pig
Authors: Geisert Rodney D , Meyer Ashley E , Pfeiffer Caroline A , Johns Destiny N , Prather Randall S , Spencer Thomas E 
Published: November 2020

Abstract
Litter size of commercial dam lines of pigs has seen a significant increase over the last 20 years. Although the current large litter size of gilts and sows can be attributed to improved genetic selection and nutrition, much of the increase in total number born has occurred through the continued increase in ovulation rate over the last four decades. Ovulation rate has increased from 14 in the 80’s to today’s average of 20–25 in commercial dam lines. However, high ovulation rate not only causes increased competition between embryos (uterine crowding), but the number of potential piglets born can out strip the number available teats. In addition, fetal crowding can reduce fetal growth and comprise health and survivability of lighter weight piglets at birth. Since there is usually minimal embryo loss observed up to blastocyst formation and hatching, the majority (20 to 30%) of embryonic loss occurs during Days 10 to 30 of gestation, which encompasses a critical period of conceptus development and differentiation, pregnancy recognition signaling, and onset of implantation. This period of early embryo loss is actually essential for establishing adequate uterine space to support fetal survival for a large litter. The period of rapid conceptus elongation on Day 12 of pregnancy provides a key “selection point” for regulating potential litter size in the pig. Establishing the fundamental roles of conceptus- and uterine-secreted factors is an essential step to develop strategies to increase placental and fetal growth to improve overall health and survivability of piglets before and after birth. During the peri-implantation period of pregnancy, porcine conceptuses produce interleukin-1B2, estrogens, prostaglandins and interferons. CRISPR/Cas9 genomic engineering technology has provided a direct method to evaluate the role of key pig conceptus genes. Loss-of-function studies have increased our understanding of the multiplicity of uterine/conceptus factors that are involved with maintenance of pregnancy.
162 Effects of bedding application on feedlot receiving phase growth performance and estimated maintenance requirement in newly weaned beef steers
Authors: Smerchek Dathan , Smith Zachary K 
Published: November 2020

Abstract
The effect of bedding application on growth performance and estimated maintenance energy requirements during the feedlot receiving phase was evaluated in newly weaned beef steers (n = 162 Charolais-Red Angus cross steers; initial un-shrunk body weight [BW] = 278 ± 13.4 kg). Steers were housed in 18 concrete surface pens (n = 9 steers/pen) measuring 7.62 × 7.62 m at the Ruminant Nutrition Center in Brookings, SD. A corn silage based receiving diet was fed that contained approximately 1.74 Mcal/kg of NEm, approximately 1.12 Mcal/kg of NEg, and monensin sodium at 27.6 g/T. Pens were assigned to one of two treatments: 1) no bedding (NO), or 2) 1.0 kg (as-is basis) of wheat straw bedding/steer/d (BED). The study was conducted from October to December of 2019. Daily ambient temperature (n = 56) averaged -3.0°C [SD 5.5] and windchill averaged -5.1°C [SD 6.1] during the course of the study. Data were analyzed using the GLIMMIX procedure of SAS 9.4 (SAS Inst. Inc., Cary, NC) as a randomized complete block design with pen serving as the experimental unit. An α of 0.05 determined significance and tendencies were declared from 0.06 to 0.10. At study initiation, initial un-shrunk body weight did not differ (P = 0.69) between treatments. Bedding application did not influence (P ≥ 0.66) final body weight (shrunk 4%) or average daily gain. Dry matter intake tended to increase (P = 0.06) by 3.8% in NO compared to BED. Gain to feed was increased (P = 0.01) by 5.6% in BED compared to NO. Maintenance coefficient (MQ) was elevated (P = 0.03) 18.9% for NO compared to BED. These data indicate that bedding application improved feed efficiency and reduced estimated MQ in beef steers during the feedlot receiving phase.
160 Effects of cow-calf management strategies on environmental footprints of beef cattle production in the United States
Authors: Baber Jessica , Wickersham Tryon , Place Sara , Rotz Al 
Published: November 2020

Abstract
As baseline environmental footprints of beef cattle production are established, mitigation strategies through cow-calf dietary and production management can be evaluated. Our objective was to quantify environmental benefits obtained by beef cattle production through implementation of cow-calf management strategies. Ten cow-calf management strategies were identified related to animal performance, feed management, or pasture management. Each strategy was incorporated into 20 representative beef cattle production systems and simulated with the Integrated Farm System Model (IFSM) using local soil and climate data. A combined strategy was identified based upon the results of individual strategies, which was also simulated with IFSM. Farm-gate life cycle assessment was used to estimate carbon (C) footprint, fossil energy use, blue water use, and total reactive nitrogen (N) loss for all production systems and strategy combinations. Averages of each environmental metric for the cow-calf sector were based on weighted averages of regional cow inventory data. National estimates of environmental impacts were based upon number of cattle represented by each production system. Feed efficiency, terminal cross sires, and reduced cow body size strategies had greatest effect on C footprint (reductions of 1.31, 1.15, and 0.71 kg CO2e/kg CW, respectively from a baseline of 16.34 kg CO2e/kg CW). Calving season, reduced cow body size, and improved fiber digestion increased fossil energy use (7.8%), while improved feed efficiency and calf implant use reduced fossil energy use (7.4%). Blue water use was reduced by a greater extent from improved feed efficiency (4.6%) and reduced cow body size (5.3%) compared to other strategies (0.6%). Total reactive N loss was reduced by 7.0 and 6.9% through improved feed efficiency and reduced cow body size, respectively. Combining strategies reduced C footprint (18.2%), fossil energy use (18.6%), blue water use (18.9%), and reactive N loss (16.2%).
161 Effects of management strategies on environmental footprints of cow-calf production in the United States
Authors: Baber Jessica , Wickersham Tryon , Place Sara , Rotz Al 
Published: November 2020

Abstract
Estimates of beef cattle production’s national and regional cradle to farm grate environmental impacts have been quantified. As baseline footprints are now established, cow-calf dietary and production management strategies can be evaluated for their capacity to reduce environmental impacts. Accordingly, our objective was to quantify environmental changes from implementing management strategies in cow-calf production. Ten management strategies were identified, with each related to animal performance, feed management, or pasture management. Each strategy was incorporated into a representative cow-calf operation for each of 6 major regions of beef production in the United States and simulated with the Integrated Farm System Model (IFSM) using local soil and climate data. A combined strategy was also identified based on results of the individual strategies, which was simulated with IFSM. Farm-gate life cycle assessment was used to estimate carbon (C) footprint, fossil energy use, blue water use, and total reactive nitrogen (N) loss for all production systems and strategy combinations. Averages of each environmental metric for the cow-calf sector were based on weighted averages of regional cow inventory data. Averaged across all strategies, the reduction in C footprint was 4.1% and fossil energy use was reduced 3.5% for the cow-calf sector. Improved feed efficiency (8.0%) and terminal cross (7.1%) strategies reduced C footprint to a greater extent than other strategies simulated (2.0%). Fossil energy use was reduced by 8.3, and 6.5% from improved feed efficiency and terminal cross strategies, respectively. Early weaning increased C footprint, fossil energy use, blue water use, and reactive N loss by 13.8, 17.8, 20.2, and 12.7%, respectively. The combined strategy, including improved feed efficiency, improved fiber digestion, calf implant use, increased weaning rate, reduced cow body weight, and terminal cross strategies, reduced C footprint (18%), fossil fuel use (18%), blue water use (23%), and reactive N loss (15%).
172 Enteric methane emissions from pregnant beef heifers as affected by multiple mitigation strategies
Authors: Teranishi Rhea E , McGeough E J , Wittenberg Karin , Crow Gary , Ominski Kim 
Published: November 2020

Abstract
This study was conducted to determine if enteric methane (CH4) emissions from pregnant beef heifers could be reduced by using multiple dietary mitigation strategies. The trial was designed as a 4 x 4 Latin square consisting of a 21-d adaptation phase followed by a 21-d data collection phase. Forty Aberdeen Angus cross pregnant beef heifers were randomly assigned to one of the four dietary treatments (n = 10): i) low protein grass hay (L; 6.1% CP; 1.9% fat); ii) adequate protein grass hay (AD; 10.8% CP; 1.9% fat); iii) AD supplemented with sunflower screenings (ADSS; 9.8% CP; 5.6% fat); and iv) above adequate protein legume grass mix hay (AAD; 12.8% CP; 1.7% fat). Total dry matter intake (DMI) was 32%, 27% and 39% greater (P &lt; 0.0001) for AD, ADSS and AAD respectively, relative to the L diet (6.5 kg d-1). Average daily gain (ADG; kg d-1) was influenced by dietary treatment (P &lt; 0.0001), as heifers offered L, AD, ADSS and AAD diets gained 0 ± 0.2, 0.6 ± 0.2, 0.5 ± 0.2 and 0.7 ± 0.2 kg d-1, respectively. Enteric CH4 emissions (L d-1), were influenced by dietary treatment (P &lt; 0.0001) with 184 ± 18.9, 214 ± 19.0, 204 ± 19.1 and 232 ± 19.1 L d-1 for heifers offered L, AD, ADSS and AAD diets, respectively. Further, heifers offered AD, ADSS and AAD diets emitted 19%, 22% and 14% less (P=0.03) enteric CH4 (% GEI) relative to diet L, respectively. This study demonstrates that supplementation to meet nutrient requirements for protein or increasing the fat content of forage-based diets using low-cost by-products such as sunflower screenings can decrease enteric CH4 emissions without adversely impacting total DMI and ADG.
166 Altering the ruminal microbiota in dairy calves using rumen contents dosing
Authors: Cox Madison S , Weimer Paul J , Steinberger Andrew J , Skarlupka Joseph H , Suen Garret 
Published: November 2020

Abstract
A major goal in dairy research is to improve milk production efficiency (MPE). With the advent of next-generation sequencing, efforts are underway to improve MPE by manipulating the rumen microbiota. MPE correlates to ruminal bacterial community composition (BCC), but the adult rumen microbiota is highly stable and returns to a baseline BCC after heavy perturbation. We seek to influence rumen BCC by early intervention in pre-weaning calves. Two cannulated Holstein donors of disparate MPE were selected. Three cohorts of 6 bull calves were established and dosed by gavage with a rumen inoculum sourced from the high-efficiency donor (HE), the low-efficiency donor (LE), or an autoclaved 50:50 mix as a microbe-free control (C). Dosing occurred at birth, then biweekly through 6 weeks. Feces were collected at each dosing. Daily preweaning intake of calf starter, which correlates to downstream feed efficiency, was greatest in HE calves and lowest in C calves (P &lt; 0.05), though preweaning average daily gain did not differ between cohorts (P = 0.210). Calves were sacrificed at 8 weeks to access rumen contents and rumen wall sections were collected to assess papillation. Fecal and rumen samples were subjected to 16S rRNA amplicon sequencing. BCC differed by cohort in fecal and rumen samples (P &lt; 0.05), with HE calf samples most similar to adult rumen samples and C calves least similar. Additionally, HE calves tended to have elongated papillae (P =0.062), the development of which is dependent on byproducts of microbial metabolism in the rumen and which points to differences in potential absorptive capacity of the epithelium. These data demonstrate that the rumen BCC can be influenced by early intervention. Future work includes expansion of this dosing protocol to a cohort of 60 female calves, following rumen BCC development and the impact on MPE in through the first lactation.