bluuebunny/crossref_metadata_2025_split huggingface dataset:
Injury Burden in the United States: Accurate, Reliable, and Timely Surveillance Using Electronic Health Care Data
Authors: Min Jae , Gurka Kelly K. , Kalesan Bindu , Bian Jiang , Prosperi Mattia 
Published: October 2019

Current injury surveillance systems in the United States, including the National Electronic Injury Surveillance System (NEISS), are unable to draw reliable subnational and subannual incidence estimates. Compared with the International Classification of Diseases (ICD), the clinical ontology system currently used widely in health care, NEISS’s coding structure lacks specificity and consistency. In parallel, the quality of ICD codes depends on accurate and complete documentation by health care providers and skillful translation into ICD codes in electronic health care data. Additionally, there is no national mandate to collect external cause of injury data. Electronic health care data, such as health records and claims, with updated codes and uniform adherence to recommendations for coding external cause of injury, have the potential to be used for a more robust and timely surveillance of injury to accurately and reliably reflect the injury burden in the United States.
Increasing Influenza and Pneumococcal Vaccination Uptake in Seniors Using Point-of-Care Informational Interventions in Primary Care in Singapore: A Pragmatic, Cluster-Randomized Crossover Trial
Authors: Ho Hanley J. , Tan Yi-Roe , Cook Alex R. , Koh Gerald , Tham Tat Yean , Anwar Eve , Hui Chiang Grace Shu , Lwin May O. , Chen Mark I. 
Published: October 2019

Objectives. To evaluate the effectiveness of point-of-care informational interventions in general practitioner clinics to improve influenza and pneumococcal vaccination uptake among elderly patients. Methods. We conducted a pragmatic, cluster-randomized crossover trial in 22 private general practitioner clinics in Singapore, from November 2017 to July 2018. We included all patients aged 65 years or older. Clinics were assigned to a 3-month intervention (flyers and posters encouraging vaccination) plus 1-month washout period, and a 4-month control period (usual care). Primary outcomes were differences in vaccination uptake rates between periods. Secondary outcomes were identification of other factors associated with vaccination uptake. Results. A total of 4378 and 4459 patients were included in the intervention and control periods, respectively. Both influenza (5.9% vs 4.8%; P = .047) and pneumococcal (5.7% vs 3.7%; P = .001) vaccination uptake rates were higher during the intervention period compared with the control period. On multilevel logistic regression analysis, follow-up for hypertension, diabetes mellitus, hyperlipidemia, or any combination of the 3 was associated with uptake of both vaccines. Conclusions. Point-of-care informational interventions likely contributed to increased influenza and pneumococcal vaccination uptake. Patients on follow-up for hypertension, diabetes mellitus, hyperlipidemia, or any combination of the 3 were more likely to receive influenza and pneumococcal vaccination and should be actively engaged by physicians. Trial Registration. ClinicalTrials.gov Identifier: NCT03445117.
Impact of the Terms “Regular” or “Pasable” as Spanish Translation for “Fair” of the Self-Rated Health Question Among US Latinos: A Randomized Experiment
Authors: Lee Sunghee , Alvarado-Leiton Fernanda , Vasquez Elizabeth , Davis Rachel E. 
Published: October 2019

Objectives. To examine measurement comparability of a Spanish version of self-rated health (SRH) with pasable as an alternative to regular for the response category “fair” in the English version.Methods. We translated “fair” into 2 Spanish versions: regular and pasable. We implemented a split-half experiment in 3 surveys independently conducted from October 2015 to January 2016, from April to November 2016, and from August to November 2017. Within each survey, we randomly assigned Spanish-interviewed Latino respondents to 1 of the 2 SRH versions. The total sample included 3261 Latino and 738 non-Latino White adults in the United States.Results. Spanish-interviewed Latinos reported substantively more favorable health on SRH with pasable than with regular. When pasable instead of regular was used for SRH, we observed a larger difference between respondents reporting positive versus negative SRH on objective health measures, including the frequency of doctor’s visits. Furthermore, when we accounted for correlates of health, Latino–White disparities were attenuated with pasable.Conclusions. We recommend using pasable instead of regular for SRH Spanish translations to improve measurement equivalence in cross-lingual and cross-cultural research.
Planejamento e implantação de uma trilha interpretativa na Mata Atlântica para atividades de educação ambiental no Instituto Federal Catarinense – Campus Rio do Sul
Authors: Milano Marja Zattoni , Krug Alessandra , Pezenti Marcelo , Funfgelt Karla , Campestrini Sergio , Fróes Eliane Henkel 
Published: October 2019

O município de Rio do Sul é o principal polo na região conhecida como Alto Vale do Itajaí, no Estado de Santa Catarina. O Alto Vale foi colonizado a partir do século XX principalmente por imigrantes alemães e italianos. Após menos de 100 anos desta ocupação, mais de 80% das florestas originais foram destruídas e hoje a região sofre com assoreamento dos rios, infertilidade dos solos, enchentes frequentes e uso abusivo de agrotóxicos. A população da região é majoritariamente urbana e, embora sofra as consequências destes problemas ambientais, parece ser incapaz de estabelecer relações diretas entre suas práticas e a crise ambiental que vivencia. Nesta realidade, o presente projeto foi implantado para apoiar a educação ambiental que vinha sendo conduzida pelas escolas do Alto Vale, buscando promover a reconexão dos cidadãos com o ambiente natural e a avaliação crítica das questões ambientais. Uma trilha interpretativa foi implantada em um remanescente de Mata Atlântica localizado no campus do Instituto Federal Catarinense de Rio do Sul. Bolsistas do projeto foram preparados para atuar como guias e educadores ambientais, atendendo turmas do ensino fundamental da região. Assim, por meio da vivência na natureza e da observação de aves, foram desenvolvidas ações voltadas à sensibilização para a conservação de florestas e da água.
Clinical and ultrasonographic evaluation of shoulder in elite waterpolo players a cross-sectional study
Authors: Ruberti Andrea , Ronga Mario , Callegari Leonardo 
Published: October 2019

Abstract
Background: Rotator cuff structural changes are common in overhead throwing athletes, such as baseball, javelin, swimming, volleyball, by increasing the risk of shoulder pain and injury. The structural alterations in elite waterpolo players are not well known. In elite waterpolo athletes there are structural alterations of rotato cuff tendons due to overuse of the dominant shoulder compared to the non-dominant, like in oth er overhead disciplines, identifiable by ultrasound (US). Methods: Asymptomatic elite men waterpolo players were selected. All athletes were evaluated with standardized scales of shoulder pain and function (Oxford Shoulder Score, Constant Shoulder Score) and provided data of training, injury and shoulder pain history. Each athlete underwent clinical evaluation of shoulders followed by US of rotator cuff, biceps and bursas. Results : 23 athletes met the inclusion criteria, mean age 24.2±3.3. 14 athletes (60.9%) presented higher degeneration grade at the dominant supraspinatus compared to the contralateral, always in the same area in outfield pla yers: anterior, lateral, pre-insertional. In 22/23 (95.6%) the biceps tendon was damaged: 20/23 athletes (87%) bilaterally, 2/23 (8.7%) at the only dominant limb. The site was always at the bicipital groove in its insertion on the humerus. Power Doppler (PD) evidenced neovascularization in only 5/23 (21.7%) players at supraspinatus: 4 bilaterally, 1 only at the non-dominant. No statistical correlation was found between tendon degeneration and age, career years. Conclusion: Outfield waterpolo players have peculiar changes in supraspinatus at preinsertional level, as seen in other overhead throwing athletes. We found changes also in biceps, bilaterally, like in shoulders of elite swimmers. We suppose that these changes are predictive of shoulder pain and injury.
Long noncoding RNA DPP10-AS1 promotes malignant processes through epigenetically activating its cognate gene DPP10 to predict poor prognosis in lung cancer patients
Authors: Tian Haihua , Pan Jinchang , Fang Shuai , Zhou Chengwei , Tian Hui , He Jinxian , Shen Weiyu , Meng Xiaodan , Jin Xiaofeng , Gong Zhaohui 
Published: October 2019

Abstract
Background Long noncoding RNAs (lncRNAs) play oncogenic or tumor-suppressive roles in various cancers. However, the epigenetic modification of lncRNA and its cognate sense gene in lung cancer remain largely unknown.Methods : qRT-PCR and Western blot were conducted to detect the expressions of DDP10-AS1 and DPP10 expression in lung cancer cell lines and tissues. The impact of DDP10-AS1 on DPP10 expression, cell growth, invasion, apoptosis and in vivo tumor growth were investigated in lung cancer cells by Western blot, rescue experiments, colony formation, flow cytometry and xenograft animal experiment.Results A novel antisense lncRNA, DPP10-AS1, is found to be highly expressed in cancer tissues and the upregulation of DPP10-AS1 predicts poor prognosis in lung cancer patients. Notably, DPP10-AS1 promotes lung cancer cell growth, colony formation, cell cycle progression and represses apoptosis in lung cancer cells by upregulating DPP10 expression. Additionally, DPP10-AS1 facilitates lung tumor growth via upregulation of DPP10 protein in xenograft mouse model. Importantly, DPP10-AS1 positively regulates DPP10 gene expression and they are coordinately upregulated in lung cancer tissues. Mechanically, DPP10-AS1 associates with DPP10 mRNA but does not enhance DPP10 mRNA stability. Hypomethylation of DPP10-AS1 and DPP10 contributes to their coordinate upregulation in lung cancer.Conclusions These findings indicate that the upregulated antisense lncRNA DPP10-AS1 promotes lung cancer malignant processes and facilitates tumorigenesis by epigenetically regulating its cognate sense gene DPP10, and DPP10-AS1 may act as a candidate prognostic biomarker and a potential therapeutic target in lung cancer.
Predicting Arteriovenous Fistula Non-Maturation in Hemodialysis Patients: Analytics of Inflammatory Markers and Serum Metabolic Values
Authors: Nakhaei Akram , Sepehri Mohammad Mehdi , Shadpour Pejman , Zadeh Morteza Khavanin 
Published: October 2019

Abstract
Background Population aging has brought a rise in the prevalence of diabetes and hypertension, leading to more cases of renal failure. Hemodialysis, as a method of renal replacement therapy, by far prevails over peritoneal dialysis (93.5% vs. 6.5%). Although arteriovenous fistula (AVF) is frequently chosen as the vascular access route for chronic hemodialysis; it has limitations including non-maturation. As maintenance of an AVF is much more costly than its creation, foreseeing maturation failure can lead to a wiser allocation of patients to AVF surgery or other alternatives, with potential for significant cost containment.Methods We investigated the relationship of routinely available systemic inflammatory markers and baseline metabolic values in 107 end-stage renal disease patients (over 35 years of age undergoing their first brachio-cephalic AVF access surgery at wrist level for chronic hemodialysis). In this study, for the first time to our knowledge, we applied predictive analytic tools such as Random Forest for retrospective analysis of prospectively collected data between 2011 and 2018.Results Our results showed that a combination of inflammatory markers and serum metabolic values can prognosticate AVF maturation outcomes with an accuracy of 0.723, by the 95% confidence interval of (0.715, 0.731) and AUC of 0.853. Also, a combination of inflammatory markers, including albumin, c-reactive protein, erythrocyte sedimentation rate, hemoglobin, lymphocytes, neutrophils, white blood cells, platelets, and red blood cell distribution width, can prognosticate AVF maturation outcomes with an accuracy of 0.674, by the 0.95 confidence interval of (0.665, 0.684) and AUC of 0.824.Conclusion Risk stratification of patients for AVF non-maturation before attempting the first AVF surgery may help prevent multiple surgical failures and costly endovascular interventions by allowing vascular surgeons to make an individualized choice of vascular access method for new patients.
Genome‐Wide Association Studies of Protein, Lutein, Vitamin C, and Fructose Concentration in Wild and Cultivated Chickpea Seeds
Authors: Karaca Nur , Ates Duygu , Nemli Seda , Ozkuru Esin , Yilmaz Hasan , Yagmur Bulent , Kartal Canan , Tosun Muzaffer , Ozdestan Ozgul , Otles Semih , Kahriman Abdullah , Chang Peter , Tanyolac Muhammed Bahattin 
Published: October 2019

ABSTRACTChickpea (Cicer arietinum L.) is one of the most important food legume crops consumed by human beings and can be used to counteract specific dietary deficiencies. Protein, lutein, vitamin C, and fructose are among the important nutritional value components of chickpea. The objectives of this study were to determine genetic variation in the concentrations of nutritional value components in the seeds of cultivated Cicer arietinum and its wild progenitor Cicer reticulatum Ladiz. and detect single nucleotide polymorphism (SNP) markers associated with these concentrations. A representative population of 180 Cicer genotypes from the two species was evaluated in two locations in Turkey in 2 yr, and the population was genotyped using 121,840 SNP markers obtained using the genotyping‐by‐sequencing analysis. The associations between the nutritional value components and loci were investigated in TASSEL software using the mixed linear model method through three different analyses. A considerable SNP stability was observed between the different year–site replicates, and 29, 10, 14, and 4 SNPs were significantly associated with protein, lutein, vitamin C, and fructose, respectively. This study provides an understanding of the genetic basis of variability in the seed concentrations of nutritional value components in chickpea. The identified markers can be used for marker‐assisted selection to improve the nutritional value components of chickpea in biofortification programs.
Controlled‐Release Nitrogen Fertilizer Improved Lodging Resistance and Potassium and Silicon Uptake of Direct‐Seeded Rice
Authors: Zhang Shugang , Yang Yuechao , Zhai Weiwei , Tong Zhaohui , Shen Tianlin , Li Yuncong C. , Zhang Min , Sigua Gilbert C. , Chen Jianqiu , Ding Fangjun 
Published: October 2019

ABSTRACTLodging is a severe problem for rice (Oryza sativa L.) because it interferes with mechanical harvesting, and potentially results total crop loss. This study investigated whether a controlled‐release urea (CRU) could reduce lodging, improve the uptake of K and Si, and yields of direct‐seeded rice. The 2‐yr field experiment included four rates of CRU (120, 180, 240, and 360 kg N ha−1), each provided in one application; a conventional urea fertilizer provided in four applications (360 kg N ha−1 total); and a control with no N fertilizer. Results indicated that the N release rates from the CRUs were matched well to N uptakes by rice plants; hence, N use efficiencies were improved. Concentrations of K and Si in rice tissues were much higher for treatments with the full and reduced rates of CRU than for the full rate urea treatment. Lodging of rice only appeared to occur in the urea treatment. Microscopic examination revealed that cells of rice stems provided with CRU treatments were more resistant to lodging than stem cells from plants in the urea treatment.
Recent Design and Structure-Activity Relationship Studies on the Modifications of DHFR Inhibitors as Anticancer Agents
Authors: Wróbel Agnieszka , Drozdowska Danuta 
Published: October 2019

Background:
Dihydrofolate reductase (DHFR) has been known for decades as a molecular
target for antibacterial, antifungal and anti-malarial treatments. This enzyme is becoming increasingly
important in the design of new anticancer drugs, which is confirmed by numerous studies including
modelling, synthesis and in vitro biological research. This review aims to present and discuss some
remarkable recent advances in the research of new DHFR inhibitors with potential anticancer activity.

Methods:
The scientific literature of the last decade on the different types of DHFR inhibitors has
been searched. The studies on design, synthesis and investigation structure-activity relationships were
summarized and divided into several subsections depending on the leading molecule and its structural
modification. Various methods of synthesis, potential anticancer activity and possible practical
applications as DHFR inhibitors of new chemical compounds were described and discussed.

Results:
This review presents the current state of knowledge on the modification of known DHFR
inhibitors and the structures and searches for about eighty new molecules, designed as potential anticancer
drugs. In addition, DHFR inhibitors acting on thymidylate synthase (TS), carbon anhydrase
(CA) and even DNA-binding are presented in this paper.

Conclusion:
Thorough physicochemical characterization and biological investigations highlight the
structure-activity relationship of DHFR inhibitors. This will enable even better design and synthesis
of active compounds, which would have the expected mechanism of action and the desired activity.
An Industry Perspective on Risks and Mitigation Strategies Associated with Post Conduct Phase of Clinical Trial
Authors: Bhagat Seema , Kapatkar Vaibhavi K. , Mane Ashish , Pinto Colette , Parikh Devang , Mittal Gaurav , Jain Rishi 
Published: October 2019

Aim/objective:
To discuss the potential risks and their mitigation strategies in the post-conduct
phase of the trial.

Background:
Risk management is very important for the clinical trial (CT) to ensure that the trial delivers
its desired outcome(s) in terms of achieving protocol objective and regulatory compliance.

Methodology:
Experienced members of the clinical operation team of a pharmaceutical major underwent
a series of sessions to identify risks associated with the post-conduct phase of CT and developed
mitigation strategies based on own experiences and guidance documents. The risks were categorized
into major, minor and critical risks.

Results:
In the post-conduct phase of a clinical trial, critical risk identified were; failure to communicate
to the stakeholder about premature termination of the trial, mismatch of statistical analysis results
and protocol objective/ regulatory requirement, clinical study report (CSR) noncompliant with
regulatory requirements or delay in CSR preparation. Safety checks suggested to mitigate these risks
including the development of related checklist or shell of relevant documents and its review by clinical
development, quality assurance (QA) and regulatory affairs (RA) team prior to finalization. Additionally,
six major and five minor risk areas were identified and safety checks were suggested.

Conclusion:
Sponsors must act proactively to ensure a systematic approach to conduct various post
conduct trial activities and the plans to mitigate the risks that could affect the quality and outcome of
the clinical trial.

Future Perspective:
A close coordination with all stakeholders for timely anticipation of risks and
execution of mitigation strategies are required for successful CT outcomes.
Comparative Transcriptome Profiling of Disruptive Technology, Single- Molecule Direct RNA Sequencing
Authors: Pradeep Chaithra , Nandan Dharam , Das Arya A. , Velayutham Dinesh 
Published: October 2019

Background:
The standard approach for transcriptomic profiling involves high
throughput short-read sequencing technology, mainly dominated by Illumina. However, the short
reads have limitations in transcriptome assembly and in obtaining full-length transcripts due to the
complex nature of transcriptomes with variable length and multiple alternative spliced isoforms.
Recent advances in long read sequencing by the Oxford Nanopore Technologies (ONT) offered
both cDNA as well as direct RNA sequencing and has brought a paradigm change in the
sequencing technology to greatly improve the assembly and expression estimates. ONT enables
molecules to be sequenced without fragmentation resulting in ultra-long read length enabling the
entire genes and transcripts to be fully characterized. The direct RNA sequencing method, in
addition, circumvents the reverse transcription and amplification steps.

Objective:
In this study, RNA sequencing methods were assessed by comparing data from Illumina
(ILM), ONT cDNA (OCD) and ONT direct RNA (ODR).

Methods:
The sensitivity & specificity of the isoform detection was determined from the data
generated by Illumina, ONT cDNA and ONT direct RNA sequencing technologies using
Saccharomyces cerevisiae as model. Comparative studies were conducted with two pipelines to
detect the isoforms, novel genes and variable gene length.

Results:
Mapping metrics and qualitative profiles for different pipelines are presented to
understand these disruptive technologies. The variability in sequencing technology and the
analysis pipeline were studied.
Lewis Acid Promoted, One-Pot Synthesis of Fluoroquinolone Clubbed 1,3,4-Thiadiazole Motifs under Microwave Irradiation: Their Biological Activities
Authors: Patel Navin B. , Parmar Rahul B. , Soni Hetal I. 
Published: October 2019

Background:
A Lewis acid promoted efficient and facile procedure for one-pot synthesis
of a novel series of fluoroquinolone clubbed with thiadiazoles motifs under microwave irradiation is
described here. This technique has more advantages such as high yield, a clean procedure, low reaction
time, simple work-up and use of Lewis acid catalyst.

Objective:
Our aim is to generate a biologically active 1,3,4- thiadiazole ring system by using a onepot
synthesis method and microwave-assisted heating. High yield and low reaction time were the
main purposes to synthesize bioactive fluoroquinolone clubbed 1,3,4- thiadiazole moiety.

Methods:
Fluoroquinolone Clubbed 1,3,4-Thiadiazole Motifs was prepared by Lewis acid promoted,
one-pot synthesis, under microwave irradiation. All the synthesized molecules were determined by
IR, 1H NMR, 13C NMR, and Mass spectra. The antimicrobial activity of synthesized compounds was
examined against two Gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa), two
Gram-positive bacteria (Staphylococcus aureus, Streptococcus pyogenes), and three fungi (Candida
albicans, Aspergillus niger, Aspergillus clavatus) using the MIC (Minimal Inhibitory Concentration)
method and antitubercular activity H37Rv using L. J. Slope Method.

Results:
Lewis acid promoted, one-pot synthesis of Fluoroquinolone clubbed 1,3,4-Thiadiazole motifs
under microwave irradiation is an extremely beneficial method because of its low reaction time
and good yield. Some of these novel derivatives showed moderate to good in vitro antibacterial, antifungal,
and antitubercular activity.

Conclusion:
One-pot synthesis of 1,3,4-Thiadiazole by using Lewis acid catalyst gives a good result
for saving time and also getting more production of novel heterocyclic compounds with good antimicrobial
properties via microwave heating method.