bluuebunny/crossref_metadata_2025_split huggingface dataset:
Immunohistochemical Expression of Vitamin D Receptor and Forkhead Box P3 in Classic Hodgkin Lymphoma: Correlation with Clinical and Pathologic Findings
Authors: Gupta Gaurav , Agrawal Tanupriya , Pilichowska Monika 
Published: April 2020

Abstract
Background Expression of forkhead box P3 (FOXP3), a key regulator of T-cell function, in the tumor immune microenvironment is related to survival in classic Hodgkin lymphoma (CHL). Vitamin D receptor (VDR), a transcription factor , agonists have been shown to induce FOXP3 expression in T-cells and enhance recruitment of these cells to the inflammatory sites. VDR expression is CHL has beenis described. However, there is no data on expression of VDR in context of quantity of FOXP3 positiveexpressing cells in CHL. Methods We examined and correlated immunohistochemical expression of VDR and FOXP3 along with clinical and pathology findings in 29 cases of CHL. Results VDR was expressed in Hodgkin Reed-Sternberg (HRS) cells and background lymphocytes and FOXP3 was expressed in background lymphocytes. 82% of CHL cases, regardless of the subtype, expressed VDR and in majority of the cases, VDR expression was directly proportional to the quantity of FOXP3 expressing lymphocytes in the tumor microenvironment. In cases with higher clinical stage (III/IV), only 28.5% of cases diffusely expressed VDR and FOXP3 compared to 71.4% showing focal positivity. Whereas in cases with lower clinical stages (I/II), the expression pattern of VDR and FOXP3 was almost similar (41.6% diffuse versus 33.3% focal). Interestingly, focal VDR and FOXP3 expression pattern was significantly higher among males. Mixed cellularity cases showed predilection for focal VDR and FOXP3 expression (80% cases); whereas nodular sclerosis subtype had focal and diffuse VDR and FOXP3 expression patterns in similar proportion. Cases with diffuse VDR and FOXP3 expression were less likely to have bone marrow involvement. Epstein Barr virus- encoded small RNA (EBER) positive cases were predominantly focally positive (80%) for VDR and FOXP3. Conclusions In summary, quantity of FOXP3 positive T-cells in CHL microenvironment seems to correlate with VDR expression. Clinical stage show a trend of inverse correlation with expression of VDR and quantity of FOXP3 positive T-cells. These findings suggest that VDR could be a possible prognostic and therapeutic target in CHL.
LRIG1 gene copy number analysis by ddPCR and correlations to clinical factors in breast cancer
Authors: Faraz Mahmood , Tellström Andreas , Ardnor Christina Edwinsdotter , Grankvist Kjell , Huminiecki Lukasz , Tavelin Björn , Henriksson Roger , Hedman Håkan , Ljuslinder Ingrid 
Published: April 2020

Abstract
Background Leucine-rich repeats and immunoglobulin-like domains 1 ( LRIG1 ) copy number alterations and unbalanced gene recombination events have been reported to occur in breast cancer. Importantly, LRIG1 loss was recently shown to predict early and late relapse in stage I-II breast cancer. Methods We developed droplet digital PCR (ddPCR) assays for the determination of relative LRIG1 copy numbers and used these assays to analyze LRIG1 in twelve healthy individuals, 34 breast tumor samples previously analyzed by fluorescence in situ hybridization (FISH), and 423 breast tumor cytosols. Results Four of the LRIG1 /reference gene assays were found to be precise and robust, showing copy number ratios close to 1 (mean, 0.984; standard deviation, +/-0.031) among the healthy control population. The correlation between the ddPCR assays and previous FISH results was low, possibly because of the different normalization strategies used. One in 34 breast tumors (2.9%) showed an unbalanced LRIG1 recombination event. LRIG1 copy number ratios were associated with the breast cancer subtype, steroid receptor status, ERBB2 status, tumor grade, and nodal status. Both LRIG1 loss and gain were associated with unfavorable metastasis-free survival; however, they did not remain significant prognostic factors after adjustment for common risk factors in the Cox regression analysis. Furthermore, LRIG1 loss was not significantly associated with survival in stage I and II cases. Conclusions Although LRIG1 gene aberrations may be important determinants of breast cancer biology, and prognostic markers, the results of this study do not verify an important role for LRIG1 copy number analyses in predicting the risk of relapse in early-stage breast cancer.
Development of a clinical prediction rule for sepsis in primary care: protocol for the TeSD-IT study
Authors: Loots Feike J. , Hopstaken Rogier , Jenniskens Kevin , Frederix Geert W.J. , de Pol Alma C. van , Bruel Ann Van den , Oosterheert Jan Jelrik , Zanten Arthur R.H. van , Smits Marleen , Verheij Theo J.M. 
Published: April 2020

Abstract
Background Early recognition and treatment of sepsis is crucial to prevent detrimental outcomes. General practitioners (GPs) are often the first healthcare providers to encounter seriously-ill patients. The aim of this study is to assess the value of clinical information and additional tests to develop a clinical prediction rule to support early diagnosis and management of sepsis by GPs. Methods We will perform a diagnostic study in the setting of out-of-hours home visits in four GP cooperatives in the Netherlands. Acutely ill adult patients suspected of a serious infection will be screened for eligibility by the GP. The following candidate predictors will be prospectively recorded: 1) age; 2) body temperature; 3) systolic blood pressure; 4) heart rate; 5) respiratory rate; 6) peripheral oxygen saturation; 7) mental status; 8) history of rigors and 9) rate of progression. After clinical assessment by the GP, blood samples will be collected in all patients to measure C-reactive protein, lactate and procalcitonin. All patients will receive care as usual. The primary outcome is presence or absence of sepsis within 72 hours after inclusion, according to an expert panel. The need for hospital treatment for any indication will be assessed by the expert panel as a secondary outcome. Multivariable logistic regression will be used to design an optimal prediction model first, and subsequently derive a simplified clinical prediction rule that enhances feasibility of using the model in daily clinical practice. Bootstrapping will be performed for internal validation of both the optimal model and simplified prediction rule. Performance of both models will be compared to existing clinical prediction rules for sepsis. Discussion This study will enable us to develop a clinical prediction rule for the recognition of sepsis in a high-risk primary care setting to aid in the decision which patients have to be immediately referred to a hospital and who can be safely treated at home. As clinical signs and blood samples will be obtained prospectively, near complete data will be available for analyses. External validation will be needed before implementation in routine care and to determine in which pre-hospital settings care can be improved using the prediction rule. Trial registration The study is registered in the Netherlands Trial Registry (registration number NTR7026).
Reduced serum VGF levels are linked with suicide risk in Chinese Han patients with major depressive disorder
Authors: Li Xingxing , Ge Huifei , Zhou Dongsheng , Wu Xiangping , Qi Gangqiao , Chen Zan , Yu Chang , Zhang Yuanyuan , Yu Haihang , wang chuang 
Published: April 2020

Abstract
Background: VGF (nonacronymic) is a neuropeptide that plays an important role in the pathogenesis of major depressive disorder (MDD). However, no studies have yet investigated VGF levels in patients with MDD who are at risk of suicide. The purpose of the present study was to determine whether serum VGF levels are related to suicide risk in patients with MMD.Methods: A total of 107 patients with MDD and 40 normal control participated in the present study. The risk of suicide was assessed using the Nurses Global Assessment of Suicide Risk (NGASR). On this basis, 60 patients were assigned to a high-risk group (NGASR≥9) and 47 were assigned to a low-risk group (NGASR<9). The severity of depression was measured using the 17-item Hamilton Depression Rating Scale (HDRS). Levels of serum VGF were determined using a double antibody sandwich enzyme-linked immunosorbent assay.Results: Serum VGF levels in the high-risk group (883.34±139.67 pg/mL) were significantly lower than in the low-risk group (1020.56±131.76 pg/mL) and in the control group (1107.00±155.38 pg/mL) (F=31.90, p<0.001). In patients with MDD, suicide risk was significantly negatively correlated with VGF levels (r = -0.55, p=0.001). Conclusions: Reduced serum VGF levels are related to risk of suicide in patients with MDD, so VGF may be a biomarker of suicide risk in MDD.
Intralesional curettage versus Prosthetic replacement,which approach is suitable for bone tumors ?------ A finite element analysis of limb salvage simulation in biomechanics
Authors: PENG Matthew Jian-Qiao , SHEN ChuLong , CHEN Hai-Yan , HU Yong , HE Er-Xing , LAU YongQiang 
Published: April 2020

Abstract
The authors have withdrawn this preprint due to author disagreement.
Orange Peel As Low-cost Adsorbent In The Elimination Of Cd(II) Ions: Kinetics, Isotherm, Thermodynamic And Optimization Evaluations
Authors: Akinhanmi Fola Temilade , Ofudje Edwin Andrew , Adeogun Idowu Abideen , Peter Aina , Ilo Joseph Mayowa 
Published: April 2020

Abstract
The presence of heavy metals in polluted water is known not only to cause stern harm to marine organisms but also to terrestial plants and animals including human beings. This research applied low-cost and environmental benign adsorbent primed from waste orange peel (OP) in the removal of Cd(II) ions from aqueous solution via the batch process. The surface properties of the orange peel powder was studied using scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (XRD) and Fourier transform infrared spectroscopy FT-IR). Operational conditions like temperature, contact time, sorbent dosage, solution pH and initial adsorbate concentration were investigated. The utmost uptake of Cd(II) ion was obtained at the contact time of 120 min, initial metal concentration of 240 mg/L, adsorbent dosage of 0.04 g/L, temperature of 45 °C and solution pH of 5.5 on the adsorption of Cd(II) ion. Equilibrium results showed that the orange peel adsorbent has an adsorption capacity of 27.916 mg/g as obtained from the Langmuir isotherm. The adsorption kinetics data followed a Pseudo-first-order kinetic model with correlation coefficient (R2) > 0.9 and low standard % error values. The adsorption process was found to be spontaneous, feasible and with enthalpy of 0.0046 kJ mol− 1 and entropy of -636.865 Jmol− 1K− 1 respectively. Results from the optimization study indicated that higher adsorbent dosage and lower Cd(II) ion concentration increased the percentage of Cd (II) ion removal. Thus, orange peel could be used in the removal of Cd(II) ion from aqueous solutions.
Minimal evisceration combined with orbital implantation
Authors: Zhang Haitao , Shen Shuhao , Yang Jiajun , Xu Zhe 
Published: April 2020

Abstract
Background: To evaluate the minimal evisceration technique, which aims to reduce surgical damage and complications and achieve better cosmetic outcomes.Methods: Eighty patients were enrolled from January 2013 to January 2019. All of the patients underwent a minimal evisceration and hydroxyapatite (HA) implant procedure, including making a lateral incision on the sclera only, transecting the optic nerve, and placing an HA implant behind the posterior sclera in the muscular cone. The implant was covered by two layers of the sclera. The main outcome measures were surgery time, blood loss, days of hospitalization, time interval for placement of prosthesis after surgery, complications, such as implant extrusion or exposure, enophthalmos, conjunctival sac stenosis, and cosmetic outcome.Results: All of the patients received minimal evisceration combined with an orbital implant. The average surgery time was 30 min, blood loss was 3 ml, days of hospitalization were 3, and the time interval for placement of the prosthesis after surgery was 4 week. No complications were recorded. The cosmetic appearance and implant mobility were satisfactory.Conclusions: This technique has advantages such as less damage, fast recovery, good effects and fewer complications. It appears to be a perfect modification for anophthalmic socket reconstruction.
Metrics of Perineal Support (MOPS) Study
Authors: Kalis Vladimir , Rušavŷ Rusavy , HAVELKOVA Linda , ZITKA Tomas , TOLAR David , Ismail Khaled M.K. 
Published: April 2020

Abstract
Background: Manual perineal protection (MPP) is an intrapartum intervention suggested to protect perineal integrity during childbirth. Proper execution of MPP is complex and evaluation of its true contribution is difficult in the clinical setting because of the large number of obstetric variables, some of which are hardly quantifiable. In this study we aimed to gather initial data on the forces executed by the accoucheur's thumb, index and middle fingers during MPP at the time of fetal head expulsion, quantify the duration of the intervention and investigate the timely interaction of the different components of MPP.Methods: Two bespoke right-handed measurement gloves (MG), with built in sensors, were designed and produced. The MG allowed the electronic real-time measurement of applied forces during MPP and transferred this data wirelessly to an integrated computer system. Sterile gloves were worn over the MG when used at the time of birth. The study was undertaken between January and December 2019. Singleton, term pregnant women having their first vaginal birth who provided a valid written consent were enrolled into this prospective pilot study. All deliveries were undertaken by one of two obstetricians experienced in MPPResults: Twenty women were enrolled. The mean duration of execution of MPP during the last contraction was 13.6 s. In 20% it lasted < 5 s. The overall means of the mean and maximum forces of the thumb, index and middle fingers were 26.7 N; 25.5 N; 20.2 N and 34.3 N; 32.6 N; and 27.6 N respectively. The onset of fingers and thumb activity was simultaneous in 13 cases (65%), while in seven (35%) deliveries the middle finger's force activity was initiated later.Conclusions: MPP during fetal head expulsion happens over a short period of time. In the majority of cases the thumb and fingers actions started simultaneously. There were differences in the duration of application and the forces executed by the fingers and thumb between the two practitioners, however this was only significant for thumb measurements. The results obtained will aid in improving further MPP modeling studies to optimize the technique.
Transmitted drug resistance mutations and subtype diversity among HIV-1 sero-positive voluntary blood donors in Accra, Ghana
Authors: Obeng Billal Musah , Bonney Evelyn Yayra , Asamoah-Akuoko Lucy , Nii-Trebi Nicholas Israel , Mawuli Gifty , Abana Christopher Zaab-Yen , Sagoe Kwamena William Coleman 
Published: April 2020

Abstract
Background: Detection of HIV-1 transmitted drug resistance (TDR) and subtype diversity (SD) are public health strategies to assess current HIV-1 regimen and ensure effective therapeutic outcomes of ART among HIV-1 patients. Globally, limited data exist on TDR and SD among blood donors. In this study, drug resistance mutations and subtype diversity among HIV-1 sero-positive blood donors in Accra, Ghana was characterized.Methods: Purposive sampling method was used to collect 81 HIV sero-positive blood samples from the Southern Area Blood Center and confirmed by serology as HIV-1 and/or HIV-2. Viral RNA was only extracted from plasma samples confirmed as HIV-1 positive. Complementary DNA (cDNA) was synthesized using the RNA as a template and subsequently amplified by nested PCR with specific primers. The expected products were verified, purified and sequenced. Neighbor-joining tree with the Kimura’s 2-parameter distances was generated with the RT sequences using Molecular Evolutionary Genetic Analysis version 6.0 (MEGA 6.0).Results: Out of the 81 plasma samples, 60 (74%) were confirmed as HIV-1 sero-positive by INNO-LIA HIVI/II Score kit with no HIV-2 and dual HIV-1/2 infections. The remaining samples, 21 (26%) were confirmed as HIV sero-negative. Of the 60 confirmed positive samples, (32) 53% and (28) 50% were successfully amplified in the RT and PR genes respectively. Nucleotide sequencing of amplified samples revealed the presence of major drug resistance mutations in two (2) samples; E138A in one sample and another with K65R. HIV-1 Subtypes including subtypes A, B, CRF02_AG and CRF09_cpx were found. Conclusion: This study found major drug resistance mutations, E138A and K65R in the RT gene that confer high level resistance to most NNRTIs and NRTI respectively. CRF02_AG was most predominant, the recorded percentage of subtype B and the evolutionary relationship inferred by phylogenetic analysis suggest possible subtype importation. The data obtained would inform the selection of drugs for ART initiation to maximize therapeutic options in drug-naïve HIV-1 patients in Ghana.
Effect of ambient air pollutants and meteorological factors on COVID-19 transmission
Authors: Jiang Ying , Xu Xiao-Long , Dai Da-Wei , Wu Xiao-Jun , Wang Jun-Yu , Wang Yun-Kun , Huang Zhen-Yu , Wang Guang-Ming , Guan Yan-Jun 
Published: April 2020

Abstract
The authors have withdrawn this preprint due to author disagreement.
Clinical observation and prognostic analysis of patients with Klebsiella pneumoniae bloodstream infection
Authors: sun tongwen , zhang shuguang , yang ziyue , sun limin , wang zhenhua , sun liutao , xu jinli , zeng li 
Published: April 2020

Abstract

Background and Purpose: The clinical prognosis of Klebsiella pneumoniae bloodstream infection is poor, and the prevalence of drug-resistant bacteria makes clinical anti-infective treatment more challenging. This retrospective study evaluated the epidemiological characteristics of patients with Klebsiella pneumoniae, the risk factors for drug-resistant bacterial infection and death, and analyzed treatment options. Methods: Clinical data of 297 patients diagnosed with Klebsiella pneumoniae bacteremia between June 2014 and June 2019 were collected.Results: Intensive care unit hospitalization history, operation history, recent antibiotic use history, mechanical ventilation, and number of days hospitalized before bloodstream infection were found to be independent risk factors for drug-resistant bacterial infection. The risk of death for carbapenem-resistant Klebsiella pneumoniae infection was 2.942 times higher than that for carbapenem-sensitive Klebsiella pneumoniae infection. For extensively drug-resistant Klebsiella pneumoniae bacteremia patients, the mortality rate of combined anti-infective therapy was lower.Conclusions: Clinicians should pay attention to patients with high-risk drug-resistant bacteria infection and administer timely anti-infection treatment. The findings of this study may provide some suggestions for early identification and standardized treatment of patients with Klebsiella pneumoniae bacteremia.
Real-world efficacy of the impulse oscillometry system for the management of bronchial asthma
Authors: Sugawara Hiroyuki , Saito Atsushi , Yokoyama Saori , Tsunematsu Kazunori , Chiba Hirofumi , Takahashi Hiroki 
Published: April 2020

Abstract
Background: Bronchial asthma (BA) has different phenotypes, and it requires a clinically effective subtype classification system. The impulse oscillometry system (IOS) is a novel device used in respiratory functional tests. However, its efficacy has not been validated. Therefore, this study aimed to assess the relationship between BA and the IOS parameters, and the difference in the therapeutic effects of inhaled corticosteroids (ICSs) among the subtype classifications was evaluated using the IOS.Methods: Of the 245 patients with bronchial asthma who were screened, 108 patients were enrolled in this study. These patients were divided based on three subtypes according to the IOS result: Central predominant type (n=34), Peripheral predominant type (n=58), and resistless type (n=16). Then, the following ICSs were randomly prescribed in daily medical care: coarse-particle ICS (fluticasone propionate [FP]), fine-particle ICS (mometasone furoate [MF]), and moderate-particle ICS (budesonide [BUD]). The treatment effects were assessed using the Asthma Health Questionnaire (AHQ) and the Asthma Control Test (ACT), and were compared among the three subtypes.Results: In the central predominant type, the AHQ score of the MF group was significantly higher than that of the FP group (15.4 vs. 3.6, p<0.01) and BUD group (15.4 vs. 8.8, p<0.05), and the ACT score of the FP group was significantly higher than that of the MF and BUD groups (24.3 vs 21.7, 22.3, respectively, p<0.05) at 4 weeks after treatment. In the peripheral predominant type, the AHQ score of the FP group was significantly higher than that of the MF group (14.1 vs. 3.4, p<0.05) and the ACT score of the FP group was lower than that of the MF and BUD groups (22.8 vs 24.6, 24.4, respectively, p<0.01) at 4 weeks after treatment. In the resistless type, no significant difference was observed in the both scores among the three groups. Conclusions: A strong association was observed between IOS subtype classification and ICS particle size in terms of therapeutic efficiency in BA. This result indicates that the ICS particle size must be identified using the IOS subtype classification before treatment.