bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mindfulness and psychological health in practitioners of Japanese martial arts: a cross-sectional study
Authors: Miyata Hiromitsu , Kobayashi Daisuke , Sonoda Akifumi , Motoike Hibiki , Akatsuka Saki 
Published: December 2020

Abstract
Background
Empirical data have suggested that mind-body practices that originated in Eastern traditions can cause desirable changes to psychological traits, the brain, somatic physiological functions, etc. Martial arts in Japan refer to the physical/mental practices that were developed based on historical combat techniques. Today, martial arts are considered activities that seek embodiment and/or mind-body unity, as well as sports. Empirical studies involving practitioners of Japanese martial arts to date remain scarce.

Methods
We conducted a questionnaire survey using a cross-sectional design to examine whether the practice of martial arts based on Japanese traditions are associated with mindfulness and psychological health. Participants included a population of practitioners of martial arts with a practice period of 0.6–35.0 years, and non-practitioners matched for demographic variables.

Results
Compared with the non-practitioners, the practitioners of martial arts had significantly higher scores for mindfulness and subjective well-being and lower scores for depression. Among the practitioners of martial arts, a longer period of practice or a higher frequency of daily practice significantly predicted higher mindfulness and psychological health.

Conclusions
The results obtained are consistent with those previously obtained for other populations of Japanese contemplatives, and support the view that practice of multiple Eastern mind-body practices might be associated with similar desirable psychological outcomes. A cross-sectional design has limitations in that it is difficult to determine the effect of continued practice, so that a longitudinal study that follows the same practitioners over time is desired in the future enquiry.
[18F]-Fluciclovine PET discrimination of recurrent intracranial metastatic disease from radiation necrosis
Authors: Parent Ephraim E. , Patel Dhruv , Nye Jonathon A. , Li Zhuo , Olson Jeffrey J. , Schuster David M. , Goodman Mark M. 
Published: December 2020

Abstract
Background
Stereotactic radiosurgery (SRS) is often the primary treatment modality for patients with intracranial metastatic disease. Despite advances in magnetic resonance imaging, including use of perfusion and diffusion sequences and molecular imaging, distinguishing radiation necrosis from progressive tumor remains a diagnostic and clinical challenge. We investigated the sensitivity and specificity of 18F-fluciclovine PET to accurately distinguish radiation necrosis from recurrent intracranial metastatic disease in patients who had previously undergone SRS.

Methods
Fluciclovine PET imaging was performed in 8 patients with a total of 15 lesions that had previously undergone SRS and had subsequent MRI and clinical features suspicious for recurrent disease. The SUVmax of each lesion and the contralateral normal brain parenchyma were summated and evaluated at four different time points (5 min, 10 min, 30 min, and 55 min). Lesions were characterized as either recurrent disease (11 of 15 lesions) or radiation necrosis (4 of 15 lesions) and confirmed with histopathological correlation (7 lesions) or through serial MRI studies (8 lesions).

Results
Time activity curve analysis found statistically greater radiotracer accumulation for all lesions, including radiation necrosis, when compared to contralateral normal brain. While the mean and median SUVmax for recurrent disease were statistically greater than those of radiation necrosis at all time points, the difference was more significant at the earlier time points (p = 0.004 at 5 min–0.025 at 55 min). Using a SUVmax threshold of ≥ 1.3, fluciclovine PET demonstrated a 100% accuracy in distinguishing recurrent disease from radiation necrosis up to 30 min after injection and an accuracy of 87% (sensitivity = 0.91, specificity = 0.75) at the last time point of 55 min. However, tumor-to-background ratios (TBRmax) were not significantly different between recurrent disease and radiation necrosis at any time point due to variable levels of fluciclovine uptake in the background brain parenchyma.

Conclusions
Fluciclovine PET may play an important role in distinguishing active intracranial metastatic lesions from radiation necrosis in patients previously treated with SRS but needs to be validated in larger studies.
The effect of preoperative chemotherapy on liver regeneration after portal vein embolization/ligation or liver resection in patients with colorectal liver metastasis: a systematic review protocol
Authors: Pavel Mihai-Calin , Casanova Raquel , Estalella Laia , Memba Robert , Llàcer-Millán Erik , Achalandabaso Mar , Julià Elisabet , Geoghegan Justin , Jorba Rosa 
Published: December 2020

AbstractIntroductionLiver resection (LR) in patients with liver metastasis from colorectal cancer remains the only curative treatment. Perioperative chemotherapy improves prognosis of these patients. However, there are concerns regarding the effect of preoperative chemotherapy on liver regeneration, which is a key event in avoiding liver failure after LR. The primary objective of this systematic review is to assess the effect of neoadjuvant chemotherapy on liver regeneration after (LR) or portal vein embolization (PVE) in patients with liver metastasis from colorectal cancer. The secondary objectives are to evaluate the impact of the type of chemotherapy, number of cycles, and time between end of treatment and procedure (LR or PVE) and to investigate whether there is an association between degree of hypertrophy and postoperative liver failure.MethodsThis meta-analysis will include studies reporting liver regeneration rates in patients submitted to LR or PVE. Pubmed, Scopus, Web of Science, Embase, and Cochrane databases will be searched. Only studies comparing neoadjuvant vs no chemotherapy, or comparing chemotherapy characteristics (bevacizumab administration, number of cycles, and time from finishing chemotherapy until intervention), will be included. We will select studies from 1990 to present. Two researchers will individually screen the identified records, according to a list of inclusion and exclusion criteria. Primary outcome will be future liver remnant regeneration rate. Bias of the studies will be evaluated with the ROBINS-I tool, and quality of evidence for all outcomes will be determined with the GRADE system. The data will be registered in a predesigned database. If selected studies are sufficiently homogeneous, we will perform a meta-analysis of reported results. In the event of a substantial heterogeneity, a qualitative systematic review will be performed.DiscussionThe results of this systematic review may help to better identify the patients affected by liver metastasis that could present low regeneration rates after neoadjuvant chemotherapy. These patients are at risk to develop liver failure after extended hepatectomies and therefore are not good candidates for such aggressive procedures.Systematic review registrationPROSPERO registration number:CRD42020178481(July 5, 2020).
KVarPredDB: a database for predicting pathogenicity of missense sequence variants of keratin genes associated with genodermatoses
Authors: Ying Yuyi , Lu Lu , Banerjee Santasree , Xu Lizhen , Zhao Qiang , Wu Hao , Li Ruiqi , Xu Xiao , Yu Hua , Neculai Dante , Xi Yongmei , Yang Fan , Qin Jiale , Li Chen 
Published: December 2020

AbstractBackgroundGermline variants of ten keratin genes (K1,K2,K5,K6A,K6B,K9,K10,K14,K16, andK17) have been reported for causing different types of genodermatoses with an autosomal dominant mode of inheritance. Among all the variants of these ten keratin genes, most of them are missense variants. Unlike pathogenic and likely pathogenic variants, understanding the clinical importance of novel missense variants or variants of uncertain significance (VUS) is the biggest challenge for clinicians or medical geneticists. Functional characterization is the only way to understand the clinical association of novel missense variants or VUS but it is time consuming, costly, and depends on the availability of patient’s samples. Existing databases report the pathogenic variants of the keratin genes, but never emphasize the systematic effects of these variants on keratin protein structure and genotype-phenotype correlation.ResultsTo address this need, we developed a comprehensive database KVarPredDB, which contains information of all ten keratin genes associated with genodermatoses. We integrated and curated 400 reported pathogenic missense variants as well as 4629 missense VUS. KVarPredDB predicts the pathogenicity of novel missense variants as well as to understand the severity of disease phenotype, based on four criteria; firstly, the difference in physico-chemical properties between the wild type and substituted amino acids; secondly, the loss of inter/intra-chain interactions; thirdly, evolutionary conservation of the wild type amino acids and lastly, the effect of the substituted amino acids in the heptad repeat. Molecular docking simulations based on resolved crystal structures were adopted to predict stability changes and get the binding energy to compare the wild type protein with the mutated one. We use this basic information to determine the structural and functional impact of novel missense variants on the keratin coiled-coil heterodimer. KVarPredDB was built under the integrative web application development framework SSM (SpringBoot, Spring MVC, MyBatis) and implemented in Java, Bootstrap, React-mutation-mapper, MySQL, Tomcat. The website can be accessed throughhttp://bioinfo.zju.edu.cn/KVarPredDB. The genomic variants and analysis results are freely available under the Creative Commons license.ConclusionsKVarPredDB provides an intuitive and user-friendly interface with computational analytical investigation for each missense variant of the keratin genes associated with genodermatoses.
Collagenous Alzheimer amyloid plaque component impacts on the compaction of amyloid-β plaques
Authors: Hashimoto Tadafumi , Fujii Daisuke , Naka Yasushi , Kashiwagi-Hakozaki Mayu , Matsuo Yuko , Matsuura Yusuke , Wakabayashi Tomoko , Iwatsubo Takeshi 
Published: December 2020

AbstractMassive deposition of amyloid β peptides (Aβ) as senile plaques (SP) characterizes the brain pathology of Alzheimer’s disease (AD). SPs exhibit a variety of morphologies, although little is known about the SP components that determine their morphology. Collagenous Alzheimer amyloid plaque component (CLAC) is one of the major non-Aβ proteinaceous components of SP amyloid in AD brains. Here we show that overexpression of CLAC precursor (CLAC-P) in the brains of APP transgenic mice results in a significant remodeling of amyloid pathology, i.e., reduction in diffuse-type amyloid plaques and an increase in compact plaques laden with thioflavin S-positive amyloid cores. In vivo microdialysis revealed a significant decrease in Aβ in the brain interstitial fluid of CLAC-P/APP double transgenic mice compared with APP transgenic mice. These findings implicate CLAC in the compaction of Aβ in amyloid plaques and the brain dynamics of Aβ.
Effects of H3.3G34V mutation on genomic H3K36 and H3K27 methylation patterns in isogenic pediatric glioma cells
Authors: Huang Tina Yi-Ting , Piunti Andrea , Qi Jin , Morgan Marc , Bartom Elizabeth , Shilatifard Ali , Saratsis Amanda M. 
Published: December 2020

AbstractHistone H3.3 mutation (H3F3A) occurs in 50% of cortical pediatric high-grade gliomas. This mutation replaces glycine 34 with arginine or valine (G34R/V), impairing SETD2 activity (H3K36-specific trimethyltransferase). Consequently, reduced H3K36me3 is observed on H3.3G34V nucleosomes relative to wild-type, contributing to genomic instability and driving a distinct gene expression signature associated with tumorigenesis. However, it is not known if this differential H3K36me3 enrichment is due to H3.3G34V mutant protein alone. Therefore, we set to elucidate the effect of H3.3G34V mutant protein in pediatric glioma on H3K36me3, H3K27me3 and H3.3 enrichment in vitro. We found that the doxycycline-inducible shRNA knockdown of mutant H3F3A encoding the H3.3G34V protein resulted in loss of H3.3G34V enrichment and increased H3K36me3 enrichment throughout the genome. After knockdown, H3.3G34V enrichment was preserved at loci observed to have the greatest H3.3G34V and H3K36me3 enrichment prior to knockdown. Induced expression of mutant H3.3G34V protein in vitro was insufficient to induce genomic H3K36me3 enrichment patterns observed in H3.3G34V mutant glioma cells. We also observed strong co-enrichment of H3.3G34V and wild-type H3.3 protein, as well as greater H3K27me3 enrichment, in cells expressing H3.3G34V. Taken together, our study demonstrates the effects of H3.3G34V mutant protein on genomic H3K36me3, H3K27me3 and H3.3 enrichment patterns in isogenic cell lines.
Microglial replacement therapy: a potential therapeutic strategy for incurable CSF1R-related leukoencephalopathy
Authors: Han Jinming , Sarlus Heela , Wszolek Zbigniew K. , Karrenbauer Virginija Danylaité , Harris Robert A. 
Published: December 2020

AbstractCSF1R-related leukoencephalopathy is an adult-onset leukoencephalopathy with axonal spheroids and pigmented glia caused by colony stimulating factor 1 receptor (CSF1R) gene mutations. The disease has a global distribution and currently has no cure. Individuals withCSF1R-related leukoencephalopathy variably present clinical symptoms including cognitive impairment, progressive neuropsychiatric and motor symptoms. CSF1R is predominantly expressed on microglia within the central nervous system (CNS), and thusCSF1R-related leukoencephalopathy is now classified as a CNS primary microgliopathy. This urgent unmet medical need could potentially be addressed by using microglia-based immunotherapies. With the rapid recent progress in the experimental microglial research field, the replacement of an empty microglial niche following microglial depletion through either conditional genetic approaches or pharmacological therapies (CSF1R inhibitors) is being studied. Furthermore, hematopoietic stem cell transplantation offers an emerging means of exchanging dysfunctional microglia with the aim of reducing brain lesions, relieving clinical symptoms and prolonging the life of patients withCSF1R-related leukoencephalopathy. This review article introduces recent advances in microglial biology andCSF1R-related leukoencephalopathy. Potential therapeutic strategies by replacing microglia in order to improve the quality of life ofCSF1R-related leukoencephalopathy patients will be presented.
Quantitative measurement of bubble textures in pumice clasts using a digital stereo microscope with low-angled ring illumination
Authors: Ohashi Masatoshi , Ichihara Mie , Maeno Fukashi , Kennedy Ben , Gravley Darren 
Published: December 2020

AbstractQuantitative analysis of bubble textures in a large number of volcanic pyroclasts is critical to investigating the eruption dynamics in a volcanic conduit. Here, we used a digital stereo microscope with low-angled ring illumination (DSM-LaRI) to measure bubble textures on unpolished cutting surfaces of pumice clasts. As the DSM-LaRI enhances brightness contrast between the bubbles (pores) and the matrix, we easily obtained the two-dimensional data on the size and shape of bubbles by image analysis. The DSM-LaRI imaging provided the distributions of size and shape of bubbles at least 50 µm across. We applied the DSM-LaRI to analyze more than 1000 pumice clasts from the 232 AD Taupo eruption and measured the mean bubble radius ($$\overline{R}$$

R
¯

) and the mean deformation degree ($$\overline{D}$$

D
¯

) in the individual clasts. The distribution of $$\overline{R}$$

R
¯

and $$\overline{D}$$

D
¯

in each layer showed a distinctive difference between the fallout and the flow deposits. These quantitative data are consistent with a qualitative classification in a previous study. Although the new DSM-LaRI method has the disadvantage of the low spatial resolution, it allows for the analysis of a large number of pumice clasts in a short time, which can address larger scale heterogeneity, by efficiently generating a large representative suite of bubble size and shape data to link bubble textures to conduit processes. This provides vital information for quantitatively modeling eruption dynamics.
Endotoxemia and circulating bacteriome in severe COVID-19 patients
Authors: Sirivongrangson Phatadon , Kulvichit Win , Payungporn Sunchai , Pisitkun Trairak , Chindamporn Ariya , Peerapornratana Sadudee , Pisitkun Prapaporn , Chitcharoen Suwalak , Sawaswong Vorthon , Worasilchai Navaporn , Kampunya Sarinya , Putcharoen Opass , Thawitsri Thammasak , Leelayuwatanakul Nophol , Kongpolprom Napplika , Phoophiboon Vorakamol , Sriprasart Thitiwat , Samransamruajkit Rujipat , Tungsanga Somkanya , Tiankanon Kanitha , Lumlertgul Nuttha , Leelahavanichkul Asada , Sriphojanart Tueboon , Tantawichien Terapong , Thisyakorn Usa , Chirathaworn Chintana , Praditpornsilpa Kearkiat , Tungsanga Kriang , Eiam-Ong Somchai , Sitprija Visith , Kellum John A. , Srisawat Nattachai 
Published: December 2020

Abstract
Background
When severe, COVID-19 shares many clinical features with bacterial sepsis. Yet, secondary bacterial infection is uncommon. However, as epithelium is injured and barrier function is lost, bacterial products entering the circulation might contribute to the pathophysiology of COVID-19.

Methods
We studied 19 adults, severely ill patients with COVID-19 infection, who were admitted to King Chulalongkorn Memorial Hospital, Bangkok, Thailand, between 13th March and 17th April 2020. Blood samples on days 1, 3, and 7 of enrollment were analyzed for endotoxin activity assay (EAA), (1 → 3)-β-d-glucan (BG), and 16S rRNA gene sequencing to determine the circulating bacteriome.

Results
Of the 19 patients, 13 were in intensive care and 10 patients received mechanical ventilation. We found 8 patients with high EAA (≥ 0.6) and about half of the patients had high serum BG levels which tended to be higher in later in the illness. Although only 1 patient had a positive blood culture, 18 of 19 patients were positive for 16S rRNA gene amplification. Proteobacteria was the most abundant phylum. The diversity of bacterial genera was decreased overtime.

Conclusions
Bacterial DNA and toxins were discovered in virtually all severely ill COVID-19 pneumonia patients. This raises a previously unrecognized concern for significant contribution of bacterial products in the pathogenesis of this disease.
An empirical approach toward the SLCP reduction targets in Asia for the mid-term climate change mitigation
Authors: Akimoto Hajime , Nagashima Tatsuya , Tanimoto Hiroshi , Klimont Zbigniew , Amann Markus 
Published: December 2020

AbstractAlthough importance of co-control of SLCPs together with the emission reduction of CO2 has attracted much attention for the mid-term climate change mitigation, the contribution to radiative forcing (RF) is rather complex, and chemistry-climate model analysis for the future scenario tends to give a “black box” for the contribution of each species. In order to deliver a more straightforward message on the effect of the reduction of SLCPs to policymakers, we propose “top-down” reduction targets of CH4 and tropospheric O3 in reference to the historical levels of their RF. Although the RF increase due to the increasing CO2 concentration is inevitable in mid-term future (ca. 0.80 W m−2 in 2040), the RF of CH4 and O3 is expected to decrease from 0.48 to 0.41, 0.34, 0.27, and 0.22 W m−2, and from 0.40 to 0.29, 0.23, 0.19, and 0.15 W m−2, respectively, if their atmospheric concentrations decrease from the level of 2010 to those of 1980, 1970, 1960, and 1950, according to the IPCC 2013 database. Consequently, the sum of ΔRFx(CH4) and ΔRFx(O3) (the difference of RF between the target year of x and 2010 as the base year) are 0.18, 0.31, 0.42, and 0.51 W m−2 in 1980, 1970, 1960, and 1950, indicating that the increase of ΔRF2040(CO2) can be compensated by 23, 39, 53, and 64%, respectively. The policy target can be selected from the combination of different target years each for CH4 and O3. With this global reduction ratio, the necessary reductions in CH4, NOx, and NMVOC in Asia were estimated and compared with the GAINS model-based cost-beneficial reduction amount proposed by the Solution Report prepared under UN Environment Asia and the Pacific Office. In order to attain the targeted reduced emission level of CH4 and NOx, new technology/practice for the reduction of livestock emission of CH4 and energy transformation from fossil fuel to renewable energy is highly advantageous for NOx reduction from industrial/power plant sources.
Malignant phyllodes tumor of the breast with rapid progression: a case report
Authors: Abe Hajime , Teramoto Atsuko , Takei Yumiko , Tanaka Yoshihito , Yoneda Genichiro 
Published: December 2020

Abstract
Background
Malignant phyllodes tumors (PTs) of the breast occur infrequently and are difficult to treat with adjuvant therapy. Here, we present a case of a female patient with a huge malignant PT with rapid progression in a short period.

Case presentation
A 44-year-old woman presented to our hospital with a rapid growth mass in her right breast, measuring 20 cm. She was initially diagnosed as having a borderline phyllodes tumor by core needle biopsy and underwent total mastectomy and artificial dermis was grafted, 20 days later, latissimus dorsi muscle flap and free skin grafting were performed. Two courses of doxorubicin–ifosfamide therapy were administered because of recurrence, but the patient died 4 months after the mastectomy.

Conclusions
A standard therapeutic strategy for malignant PTs is needed in urgently to reduce the risk of tumor recurrence.