bluuebunny/crossref_metadata_2025_split huggingface dataset:
Expression of microRNA in follicular fluid in women with and without PCOS
Authors: Butler Alexandra E. , Ramachandran Vimal , Hayat Shahina , Dargham Soha R. , Cunningham Thomas Keith , Benurwar Manasi , Sathyapalan Thozhukat , Najafi-Shoushtari S. Hani , Atkin Stephen L. 
Published: November 2019

AbstractSeveral studies have shown the expression of small non-coding microRNA (miRNA) changes in PCOS and their expression in follicular fluid has been described, though the number of studies remains small. In this prospective cohort study, miRNA were measured using quantitative polymerase chain reaction (qPCR) in 29 weight and aged matched anovulatory women with PCOS and 30 women without from follicular fluid taken at the time of oocyte retrieval who were undergoingin vitrofertilization(IVF); miRNA levels were determined from a miRNA data set. 176 miRNA were detected, of which 29 differed significantly between normal women and PCOS women. Of these, the top 7 (p < 0.015) were miR-381-3p, miR-199b-5p, miR-93-3p, miR-361-3p, miR-127-3p, miR-382-5p, miR-425-3p. In PCOS, miR-382-5p correlated with age and free androgen index (FAI), miR-199b-5p correlated with anti-mullerian hormone (AMH) and miR-93-3p correlated with C-reactive protein (CRP). In normal controls, miR-127-3p, miR-382-5p and miR-425-3p correlated with the fertilisation rate; miR-127-3p correlated with insulin resistance and miR-381-3p correlated with FAI. Ingenuity pathway assessment revealed that 12 of the significantly altered miRNA related to reproductive pathways, 12 miRNA related to the inflammatory disease pathway and 6 were implicated in benign pelvic disease. MiRNAs differed in the follicular fluid between PCOS and normal control women, correlating with age, FAI, inflammation and AMH in PCOS, and with BMI, fertilization rate (3 miRNA), insulin resistance, FAI and inflammation in control women, according to Ingenuity Pathway Analysis.
Gut microbiome of treatment-naïve MS patients of different ethnicities early in disease course
Authors: Ventura R. E. , Iizumi T. , Battaglia T. , Liu Menghan , Perez-Perez G. I. , Herbert J. , Blaser M. J. 
Published: November 2019

AbstractAlthough the intestinal microbiome has been increasingly implicated in autoimmune diseases, much is unknown about its roles in Multiple Sclerosis (MS). Our aim was to compare the microbiome between treatment-naïve MS subjects early in their disease course and controls, and between Caucasian (CA), Hispanic (HA), and African American (AA) MS subjects. From fecal samples, we performed 16S rRNA V4 sequencing and analysis from 45 MS subjects (15 CA, 16 HA, 14 AA) and 44 matched healthy controls, and whole metagenomic shotgun sequencing from 24 MS subjects (all newly diagnosed, treatment-naïve, and steroid-free) and 24 controls. In all three ethnic groups, there was an increased relative abundance of the same single genus, Clostridium, compared to ethnicity-matched controls. Analysis of microbiota networks showed significant changes in the network characteristics between combined MS cohorts and controls, suggesting global differences not restricted to individual taxa. Metagenomic analysis revealed significant enrichment of individual species within Clostridia as well as particular functional pathways in the MS subjects. The increased relative abundance of Clostridia in all three early MS cohorts compared to controls provides candidate taxa for further study as biomarkers or as etiologic agents in MS.
Geodynamic monitoring and its maintenance using modeling by numerical and similar materials methods
Authors: Morozov Konstantin , Shabarov Arkady , Kuranov Anton , Belyakov Nikita , Zuyev Boris , Vlasenko Dmitriy , Demekhin Dmitriy , Bakhtin Eugene 
Published: November 2019

The paper describes the fundamental issues of deformation monitoring systems and instrumental methods for measuring the stress-strain state of the rock massif based on the use of three-component strain sensors developed by specialists from the University of Mines and Avangard OJSC.
One of the main tasks of the developed systems is the prediction and prevention of possible dynamic manifestations of rock pressure in rockburst-hazardous deposits.
Simulation of precious metals recovery from nickel smelter slag under high-pressure oxidative acid leaching (HPOXAL)
Authors: Cahyari Khamdan , Kholik  
Published: November 2019

Abstract
The nickel smelter slag as a solid waste of pyrometallurgical operations can be harmful to the environment especially against the ground that will be contaminated by hazardous components contained the smelter slag. Nickel smelter slag has the content of valuable metals as the following: Nickel (Ni), Cobalt (Co), Copper (Cu), Iron (Fe) and Zinc (Zn). Those valuable metals were carried out by high-pressure oxidative acid leaching to recover all the valuable metals suitable with the high measures of recovery. High-pressure oxidative acid leaching (HPOXAL) is a suitable process for the nickel smelter slag with the parameters of the leaching process as the following: the particle size about – 150 + 74 μm, sulfuric acid solution with the concentration of 0.3 mol in 1 liter solution, Ratio of Liquid to Solid (L/S) with 7 ml of liquid to 1 gram of solid components, oxygen overpressure at 600 kPa, operating temperature about 180 - 200 °C and the retention time of 80 minutes. The measures recovery of this leaching process for each valuable metal in percent of initial weight such as nickel and cobalt about 97%, copper about 95%, 94% of zinc and all iron can be separated, respectively. At the separation section, LIX 984N as extractant extracts copper from the product (leaching solution) of the reactor, limestone was used to capture iron in the leaching solution, versatic 10 and decyl-4PC were conducted to remove undesirable mineral that was generated from the leaching process, and cyanex 272 extractants to extract such as nickel, cobalt, and zinc, respectively. This simulation used the feed flowrate with 150 tons per day. The results of the precious metals product were generated each the mass rate such as copper, nickel, cobalt, zinc, and iron with 927 kg/day, 2147 kg/day, 875 kg/day, 199 kg/day and 71,000 kg/day, respectively.
Enhancing the yield and quality of Kemiri Sunan crude oil by preliminary extraction of feedstock
Authors: Supriyadi S , Purwanto P P , Hermawan H 
Published: November 2019

Abstract
Biodiesel is an alternative fuel produced by chemical reaction between vegetable oil or animal fat with alcohol and catalyst. The preliminary treatment in extraction of biodiesel feedstock is an important process to obtain maximum yield of crude oil. This study aims to find optimal preliminary treatment on extraction of Kemiri Sunan seed in order to produce crude oil with maximum yield and quality. The research investigated the effects of screw press machine rotation and duration of steaming process. Three levels of duration of steaming used were 30, 60 and 90 minutes. Observations of crude oil yield, moisture content, free fatty acid content, kinematic viscosity, density and color were done. The result suggested that the rotation of screw press machine affected the crude oil yield. The duration of steaming process affected the oil yield, free fatty acid content, kinematic viscosity, density and color. The best oil yield result of 70.3% was obtained from the combination of heating duration of 60’ and screw rotation of 25 RPM.
Effect of Sago Starch Concentrations, Stirring Speeds, and Lemongrass Oil Concentration for Edible Film Production Using Solvent Casting Method
Authors: Santosa H , Djaeni M. , Ratnawati  , Rokhati N , Setiatun A P , Afriyanti  
Published: November 2019

Abstract
Foods such as meat, fillet, fruit and vegetable tend to have short shelf life when it is stored outside without packaging. Recently, edible film and coating become a potential alternative for food packaging. Edible film brings a lot of advantages from environmental aspect because it is easier to be decomposed rather than the synthetic film. Furthermore, edible film might be produced from both natural raw material and waste materials. Edible film from natural raw material gives a rigid structure. Another additive agent must be used to make a better quality product, such as glycerol and lemon grass oil as plasticizer and antimicrobial agent, respectively. These materials are commonly used in edible film production due to its capability for extending the food shelf-life by inhibiting bacterial growth. The objectives of this study are to investigate the effect of starch concentration (3% to 10% w/v), stirring speed (350, 700, 1100 rpm) and lemon grass oil concentration (1%, 5% v/v) on tensile strength, water vapor permeability, and antimicrobial activity. Results show that 4% (w/v) sago starch gives the best edible film mechanical properties and least water transfer. Edible film with 4% (w/v) sago starch, 8 rpm constant stirring, and 5% (v/v) lemongrass oil gives the best result in tensile strength, water vapor permeability and bacterial growth. In addition, variables of starch concentration, stirring speed and lemon grass oil concentration are interrelated to produce the best edible film.
Comparison of the effectiveness electrocoagulation of dye (batik waste water) using iron and zinc as an anodes
Authors: Suhartana S. , Purwanto P. , Darmawan Adi 
Published: November 2019

Abstract
This study aims to obtain dye (batik waste water) treatment by electrocoagulation with optimal results. The performance of electrocoagulation process was carried out in batch reactor. Four variables were taken which thought to greatly influence the electrocoagulation process, such as: distance between electrodes, voltage, acidity (pH) and electrolysis time. In this process, samples of 20 mL were taken out from the batch at 5, 10, 15, 20, 25, 30, 35, 40, 45 dan 50 minutes of contact time. Results obtained show that the most effective removal efficiency could be achieved at alkaline condition. Research that has been done shows that the percentage of wastewater treatment results is strongly influenced by the four variable changes. The study demonstrated that the lowest degradation efficiency occours at acid pH, while an increase temperature has only a slight effect on the electrocoagulation process. The maximum results from the electrocoagulation processes was obtained, with determine by measurement of chemical oxygen demand (COD). The results showed that, iron is better used as an anode than zinc. The effectiveness electrocoagulation reached optimal at 96.54% (at Fe anode) and 81.65% (at Zn anode), at alkaline condition (pH > 9), electrolysis time of 30 minutes, electrode distance of 1 cm and temperature at 70°C.
The Ultrasound-Assisted Extraction of Rice Bran Oil with n-Hexane as a Solvent
Authors: Djaeni M , Listyadevi Y L 
Published: November 2019

Abstract
Rice bran oil (RBO) is one of edible oil which widely used in chemical, pharmaceutical, and food industries due to its unique properties and high medicinal value. RBO obtained by ultrasound-assisted extraction using n-hexane as solvent. This present study aims to determine the ideal bran to solvent ratio to obtain the highest extraction yield using optimal temperature. Rice bran which 90% passable through a sieve with 700 μm opening, was used for the extraction process. The extraction process was done at various temperature (40, 50, 60°C), and various bran to solvent ratio (1:5, 1:10, 1:15 w/v) for 2 hours at 40 kW. The highest yield ultrasound-assisted extraction was 20.35% with ideal bran to solvent ratio 1:5 w/v using 60°C. The RBO yield increased with increasing bran to solvent ratio and temperature at the isobaric condition.
Fructose Syrup Production from Tapioca Solid Waste (Onggok) By Using Enzymatic Hydrolysis in Various pH And Isomerization Process
Authors: Yulistiani F , Saripudin  , Maulani L , Ramdhayani W S , Wibisono W , Permanasari A R 
Published: November 2019

Abstract
Tapioca solid waste (onggok) from cassava contains high carbohydrate (about 76%). The carbohydrate contains amylose and amylopectin which are the raw material of glucose syrup. Fructose is isomer of glucose which has higher dextrose equivalent. It is potential to produce fructose syrup from onggok by using enzymatic hydrolysis and through isomerization process. This research aimed to produce fructose syrup from cassava solid waste in various pH of enzymatic hydrolysis (liquefaction and saccharification) and isomerizing the glucose to be fructose syrup. Onggok in particle size 63 μm was dissolved in distilled water to make substrate concentration of 25%, 30%, and 35% (w/v). Each of the substrate concentration was hydrolyzed with α-amylase as the liquefaction process and was continued with addition of glucoamylase in saccharification process. The hydrolysis process was set in various pH, 5.5, 6, and 6.5. In liquefaction, the substrate suspension was added by 0.067% α-amylase (v/v) with heating temperature 90°C for 60 min. Then, it was continued for saccharification by adding glucoamylase in the same ratio with α-amylase for 120 minutes in 55°C. During the hydrolysis process, the sample was taken from each solution in 10 minutes to analyze the reducing sugar concentration by DNS reagent. After the hydrolysis process, the suspension was filtered and evaporated to reduce the water content. Filtrate with the highest reducing sugar concentration was isomerized by adding 1% of glucose isomerase and heated in 60°C, pH 8.2 for 43 hours. The fructose syrup concentration was then analyzed by resorcinol reagent and the result was 229.3 g/l, yield 9.298%, and conversion 24.587%.
Betaine-based deep eutectic solvents with diol, acid and amine hydrogen bond donors for carbon dioxide absorption
Authors: Mulia K , Krisanti E , Nasruddin  , Libriandy E 
Published: November 2019

Abstract
Deep eutectic solvents (DESs) are known as alternative green solvents due to their ease of synthesis, availability, biodegradability and negligible volatility. DESs consisted of betaine as the hydrogen bond acceptor with 1,2-propanediol, levulinic acid and methyl diethanolamine (MDEA) as the hydrogen bond donors, were prepared in 1:3 and 1:6 molar ratios. The carbon dioxide solubilities in these DESs were determined using a volumetric apparatus at 313.15 K and 6 bar. The results show that CO2 solubilities in betaine-based DESs are higher than the reported CO2 solubilities in choline chloride-based DESs, for DESs having the same hydrogen bonding donor and an identical or similar composition. The viscosity and polarity of hydrogen bond donors affected the ability of deep eutectic solvent to absorb carbon dioxide. The betaine-MDEA deep eutectic solvent, with molar ratio of 1:6, has the highest absorption capacity of 0.0814 mol CO2/mol DES, measured at 313.15 K and 6 bar.
The Reduction of COD Levels in Domestic Waste Water Using Combination of Activated Sludge Methode – Activated Carbon Continously
Authors: Mukhtar G. , Retno I , M T. , Fajar N , Satria A 
Published: November 2019

Abstract
Domestic waste water produced from Pujasera Politeknik Negeri Bandung has a relatively high organic compound. It can cause environmental pollution, so waste water treatment is required to reduce the concentration of organic compounds. The purpose of this study is to determine the efficiency of processing organic compounds in domestic wastewater using active sludge in combination with Activated carbon. The acclimation process is carried out in batches, while the waste water feed is continuously fed by varying the retention time and activated carbon concentration. Variation of retention time of 12, 18, 24 hours and variation of activated carbon concentration 50, 100, 150 ppm. The parameters used in aerobic wastewater treatment are COD, pH, and MLSS measurements. Based on the results of data analysis showed that the percentage decrease of COD from 861 mg / L to 160 mg / L with a pH range of 6.5 to 7 and MLSS averaged 2951 mg / L. The optimum retention time 24 hours with efficiency of COD effluent reduction without activated carbon was 74.91%. With the same operating conditions, at 24 hours of retention time using a combination of activated sludge - activated carbon of 150 ppm, the efficiency of COD effluent reduction was 89.97%.
Recycling and processing of solid waste into products of the cosmetic packaging industry
Authors: Purwanto P. , Permana-Citra A.D. 
Published: November 2019

Abstract
The waste generation of the plastic packaging industry for the cosmetic product is consisting of the hazardous and non-hazardous waste. The non-hazardous waste of cosmetic product includes product reject, plastic bags, cardboard, and paper. The content of hazardous waste of the cosmetic product is the part such as chemical packaging drum, paint sludge, and wastewater treatment sludge. On the other hand, there is a chance of the waste management that can be started from the segregation of both hazardous and non-hazardous waste, recycling of non-hazardous waste and utilization of waste into products by third parties. That is because the dangerous waste that is carried out according to the waste regulation can be used as a useful product to provide economic and environmental benefits. The research examines various opportunities to use waste as raw material and also to be processed into products. The study steps consist of identification, assessment, techno-economic analysis, and implementation approaches. The study results show that the hazardous solid waste can be used as fuel, filling materials for paving blocks, and ceramic raw materials. The utilization as raw material for paving blocks shows the most significant opportunity for economic and has environmental benefits.
Drying rate and efficiency energy analysis of paddy drying using dehumidification with zeolite
Authors: Djaeni M , Irfandy F , Utari F D 
Published: November 2019

Abstract
Paddy must be dried to prolong the storage life and maintain the quality. There was some researches about paddy drying system: bin dryer. The dehumidification process giving advantages in high efficiency energy, but it is taking the heat for regeneration. Based on the literature study, it is hard to find the literature about drying efficiency analysis during the drying of paddy. This research aims to observe the effect of drying temperatures and addition of zeolite in drying system. Paddy grains were dried from initial moisture content 22% (wet basis) under different temperature 40, 50, and 60°C for 90 minutes drying. Zeolite was used as adsorbent to decrease the relative humidity of air dryer. Results showed that use of zeolite lowering the air humidity and increasing driving force for drying. These made the drying rate increased. The higher drying temperature can also increase drying rate. Energy efficiency decrease during the drying process. Also, addition of zeolite in drying process affected the drying efficiency.
Effect of pH on Depolymerization of κ-Carrageenan by Ultrasound, Ozone and Their Combination
Authors: Prasetyaningrum A , Jos B , Dharmawan Y , Ratnawati R , Riyandita S , Scesario R 
Published: November 2019

Abstract
κ-carrageenan is sulphated linear polysaccharides of D-galactose and 3,6-anhydro-D-galactose extracted from certain red seaweeds of the Rhodophyceae class. They have been extensively used in the food and pharmaceutical industry. However, the use of the κ-carrageenan in the biomedical field is inhibited by its easy to form a gel and a high viscosity. Therefore, it is necessary to production of low molecular weight of carrageenan using depolymerization methods. The objective of this research is to study the effect of pH to reduced the molecular weight of κ-carrageenan with ozonation (O3), ultrasonication (US) and the combination of O3 and US (O3–US) methods. The experimental result shows that the O3–US treatment can decrease the molecular weight of κ-carrageenan until 80.77% at pH 7 during 20 minutes of treatment. Depolymerization of κ-carrageenan at pH 3 has the highest percentage compared to pH 7 and pH 10. The percentage depolymerization of κ-carrageenan using O3–US method at pH 3,7 and 10 are 94.22%; 80.77% and 75.85%, respectively.
The Effect of pH and Current Density on Electrocoagulation Process for Degradation of Chromium (VI) in Plating Industrial Wastewater
Authors: Prasetyaningrum A , Jos B , Dharmawan Y , Praptyana I R 
Published: November 2019

Abstract
The heavy metal of chromium is one of the most common pollutants of the plating industrial wastewater. Cr (VI) is one of toxic metal that cause serious threat to human health and the environment due to its cumulative effects and non-degradability. Among the technologies for removing these pollutants, electrocoagulation can be considered as an effective method. This method has some advantages such as fewer amounts of produced sludge and high efficiency in removal of pollutants. This research intended to study effect initial pH and current density on the degree of Cr (VI) removal from wastewater of plating industry by using the electrocoagulation method. The process is done at pH values of (3-9) at current density of 0.42, 0.63, 0.83, 1.04, and 1.25 mA/cm2. Synthetic chromium wastewater was prepared at the initial concentration of 50 mg L−1 during 30 minutes of electrocoagulation process. After electrocoagulation treatment, concentration of Cr (VI) analysed by UV-Vis spectrophotometer. The result revealed that the best removal was achieved at pH 7 and current density of 1.25mA/cm2.
The Effects of Rice Straw Weight Fraction and Particle Size on Thermal Conductivity and Mechanical Properties of Polypropylene Composite
Authors: Wahyuni N L E , Soeswanto B , Aulia H , Fadhilah R.A 
Published: November 2019

Abstract
Polypropylene is a widely used material for various types of equipment. As a recyclable thermoplastic polymer, polypropylene composite provides an attractive quality of thermal insulation due to its heat-retaining property. The use of rice straw for filler in thermal insulating material is based on its low thermal conductivity, renewable material, and low cost production techniques. The composite were manufactured by melt-mixing of neat polypropylene and filler of various weight fractions, pellet formation, followed by reprocessing with injection molding. The current work focused on determining the effect of filler’s weight fraction, particle size and pretreatment on the thermal conductivity and tensile strength of the composite. The result showed that an increase of filler’s weight fraction up to 30% decreased the composite’s thermal conductivity and tensile strength gradually. Composite of fine particles filler (325 mesh) gave a lower thermal conductivity and higher tensile strength than that of coarse particle (18-35 mesh). Composite with the lowest thermal conductivity of 1.39 W/mK was obtained at 30 w.% filler content and fine particles filler (325 mesh). Improvement on composite properties achieved by acetylated rice straw which enable to reduce straw’s hydophylicity and enhance adhesion between matrix and filler.