bluuebunny/crossref_metadata_2025_split huggingface dataset:
A 360-Degree Examination of the Placement Fit of Medical Clowns
Authors: Efrat-Treister Dorit , Altman Daniel , Friedmann Enav , Margalit Dalit Lev-Arai , Teodorescu Kinneret 
Published: July 2020

Abstract
Background – Most existing research on medical clowns in health care service has investigated their usefulness among child health consumers. In a 360-degree research stream, we aim to identify the optimal audience (adults or children health consumers), for which medical clowns are most useful in enhancing health consumers’ satisfaction and, in turn, reducing their aggressive tendencies.Methods – We conducted three studies, which examined the placement fit of medical clowns from a different point of view: medical staff (Study 1a, n = 88), medical clowns (Study 1b, n = 20), and health consumers (Study 2, n = 397).Results – Studies 1a and 1b demonstrate that both medical staff and clowns believe that child health consumers profit most from the clowns. In Study 2, data from health consumers in seven different hospital wards showed that clowns are useful in mitigating the effect of negative affectivity on satisfaction, thereby reducing aggressive tendencies among children. Surprisingly, the effect of medical clowns on adults is not only weaker, but reversed, such that interactions with medical clowns decrease adults’ satisfaction and increase their aggressive tendencies.Discussion - The medical clowns are most useful in elevating satisfaction and reducing aggressive tendencies of children. However, older adults show lower satisfaction and higher aggressive tendencies following the performance of the medical clown. The main limitation of the study is investigating aggressive tendencies rather than actual aggression. Future research should examine actual aggression.Conclusion – Medical clowns should be placed in children’s wards. This conclusion can guide health care service policy makers by indicating the optimal placement of clowns, thereby benefitting most from the clowns’ efforts, elevating health consumer satisfaction, and reducing aggressive tendencies.Trial registration – article doesn’t report a health care intervention on human participants.
Wdr47, Camsaps, and Katanin cooperate to generate ciliary central microtubules
Authors: Liu Hao , Zheng Jianqun , Zhu Lei , Chen Yawen , Zhang Yirong , xie Lele , Zhang Wei , Zhou Jun , Yan Xiumin , Zhu Xueliang 
Published: July 2020

Abstract
The axonemal central pair (CP) are non-centrosomal microtubules critical for planar ciliary beat. How they form, however, is poorly understood. Here, we show that mammalian CP formation requires cooperative activities of Katanin, Camsaps, and Wdr47. Katanin severs peripheral microtubules to produce central microtubule seeds in nascent cilia. Camsaps stabilize minus ends of the seeds to facilitate MT outgrowth, whereas Wdr47 concentrates Camsaps into the axonemal central lumen to properly position the central microtubules. Wdr47 deficiency in mouse multicilia results in complete loss of CP, rotatory beat, and primary ciliary dyskinesia. Overexpression of Camsaps induces central microtubules in Wdr47-/- ependymal cells but at the expense of low efficiency, abnormal numbers, and wrong location, whereas overexpression of a dominant inhibitor of Katanin impairs the CP formation. We propose that Wdr47, Camsaps, and Katanin constitute a general cooperative work team for the generation of non-centrosomal MT arrays in polarized subcellular compartments.
Identification of cold stress responsive microRNAs in cold tolerant and susceptible Hemerocallis fulva by high throughput sequencing
Authors: Huang Changbing , Jiang Chun , Jin limin , Zhang Huanchao 
Published: July 2020

Abstract
Background:Hemerocallis fulva is a perennial herb belonging to Hemerocallis of Hemerocallis. Because of the large and bright colors, it is often used as a garden ornamental plant. But most varieties of H. fulva on the market will wither in winter, which will affect their beauty. It is very important to study the effect of low temperature stress on the physiological indexes of H. fulva and understand the cold tolerance of different H. fulva. MiRNA is a kind of endogenous non coding small molecular RNA with length of 21-24nt. It mainly inhibits protein translation by cutting target genes, and plays an important role in the development of organisms, gene expression and biological stress. Low temperature is the main abiotic stress affecting the production of H. fulva in China, which hinders the growth and development of plants. A comprehensive understanding of the expression pattern of microRNA in H. fulva under low temperature stress can improve our understanding of microRNA mediated stress response. Although there are many studies on miRNAs of various plants under cold stress at home and abroad, there are few studies on miRNAs related to cold stress of H. fulva. It is of great significance to explore the cold stress resistant gene resources of H. fulva, especially the identification and functional research of miRNA closely related to cold stress, for the breeding of excellent H. fulva.Results A total of 5619 cold-responsive miRNAs, 315 putative novel and 5 304 conserved miRNAs, were identified from the leaves and roots of two different varieties ‘Jinyan’ (cold-tolerant) and ‘Lucretius ’ (cold-sensitive), which were stressed under -4 oC for 24 h. Twelve conserved and three novel miRNAs (novel-miR10, novel-miR19 and novel-miR48) were differentially expressed in leaves of ‘Jinyan’ under cold stress. Novel-miR19, novel-miR29 and novel-miR30 were up-regulated in roots of ‘Jinyan’ under cold stress. Thirteen and two conserved miRNAs were deferentially expressed in leaves and roots of ‘Lucretius’ after cold stress. The deferentially expressed miRNAs between two cultivars under cold stress include novel miRNAs and the members of the miR156, miR166 and miR319 families. A total of 6 598 target genes for 6 516 known miRNAs and 82 novel miRNAs were predicted by bioinformatic analysis, mainly involved in metabolic processes and stress responses. Ten differentially expressed miRNAs and predicted target genes were confirmed by quantitative reverse transcription PCR(q-PCR), and the expressional changes of target genes were negatively correlated to differentially expressed miRNAs. Our data indicated that some candidate miRNAs (e.g., miR156a-3-p, miR319a, and novel-miR19) may play important roles in plant response to cold stress.﻿Conclusions Our study indicates that some putative target genes and miRNA mediated metabolic processes and stress responses are significant to cold tolerance in H. fulva.
Comparison of the central human and mouse platelet signaling cascade by systems biological analysis
Authors: Balkenhol Johannes , Kaltdorf Kristin V. , Mammadova-Bach Elmina , Braun Attila , Nieswandt Bernhard , Dittrich Marcus , Dandekar Thomas 
Published: July 2020

Abstract
Background
Understanding the molecular mechanisms of platelet activation and aggregation is of high interest for basic and clinical hemostasis and thrombosis research. The central platelet protein interaction network is involved in major responses to exogenous factors. This is defined by systemsbiological pathway analysis as the central regulating signaling cascade of platelets (CC).
Results
The CC is systematically compared here between mouse and human and major differences were found. Genetic differences were analysed comparing orthologous human and mouse genes. We next analyzed different expression levels of mRNAs. Considering 4 mouse and 7 human high quality proteome data sets, we identified then those major mRNA expression differences (81%) which were supported by proteome data. CC is conserved regarding genetic completeness, but we observed major differences in mRNA and protein levels between both species. Looking at central interactors, human PLCB2, MMP9, BDNF, ITPR3 and SLC25A6 (always uniprot notation) show absence in all murine datasets. CC interactors GNG12, PRKCE and ADCY9 occur only in mice. Looking at the common proteins, TLN1, CALM3, PRKCB, APP, SOD2 and TIMP1 are higher abundant in human, whereas RASGRP2, ITGB2, MYL9, EIF4EBP1, ADAM17, ARRB2, CD9 and ZYX are higher abundant in mouse. Pivotal kinase SRC shows different regulation on mRNA and protein level as well as ADP receptor P2RY12.
Conclusions
Our results highlight species-specific differences in platelet signaling and points of specific fine-tuning in human platelets as well as murine-specific signalling differences.
The Need for Additional Mental Health Support for Women in the Postpartum Period in the Times of Epidemic Crisis
Authors: Chrzan-Dętkoś Magdalena , Walczak-Kozłowska Tamara Zofia , Lipowska Małgorzata 
Published: July 2020

Abstract
Background: This study aimed to identify possible intensification of mental health difficulties among women seeking support in the postpartum period during the epidemic state in Poland. We assumed that the epidemic crisis, social isolation and restrictions in hospitals which affect pregnant and postpartum women - lack of family labours, lack of the possibility to be with the newborn when he/she is hospitalized, may increase fear and reduce psychosocial resources of women, hinder their normal process of transition to motherhood and thus contribute to the intensified severity of depressive symptoms.Methods: The study participants were women seeking support at the on-line platform of the project ‘Next Stop: MUM’, which is a part of the postpartum depression prevention’s program implemented by the Ministry of Health in Poland, and enables remote self-screening for the severity of the postpartum depression symptoms with the Edinburgh Postpartum Depression Scale developed by Cox and collaborators. The analyzed data in this study were obtained from 139 women: 61 filled forms from October 1 - November 10, 2019 (non-epidemic period), and 78 filled forms from February 20 - March 30 (beginning of the epidemic), 2020.Results: A statistically significant difference in the severity of postpartum depression symptoms was observed among women making a self-assessment with EPDS scale at the beginning of the covid-19 epidemic in Poland (M = 15.71; SD = 6.23), compared to the pre-epidemic neutral period (M = 13.56; SD = 6.46).Conclusions: The results of this study indicate that the epidemic crisis may be associated with an increased need for additional caution and support of women's mental health in the postpartum period. We believe that recommendations for medical staff, policy and families of women struggling with postpartum depression symptoms during crisis should be widespread as the second wave of covid-19 disease may develop in autumn 2020.
Natural Hybridization between Two Butterfly Bushes in Tibet: Dominance of F1 Hybrids Promotes Strong Reproductive Isolation
Authors: Liao Rongli , Sun Weibang , Ma Yongpeng 
Published: July 2020

Abstract
Background: F1 hybrids acting as a bridgehead for producing later generation hybrids can have evolutionary significance through strengthening reproductive isolation or facilitating gene flow between parental species, depending on whether backcrossing can occur. It had been suggested that the Tibetan plant Buddleja wardii was a hybrid species between B. alternifolia and B. crispa based on their sympatric distributions and the morphological characters in last century. Till now however, we still have limited evidence to prove key issues to B. wardii, like if it is of hybrid origin indeed and whether it is currently a true hybrid species already.Results: In the present study, two sympatric populations of these three taxa were examined and compared using four nuclear genes and three chloroplast intergenic spacers, as well as with 10 morphological characters. Our results suggest that at both sites B. × wardii was likely to be a hybrids between B. alternifolia and B. crispa, and moreover, most of the hybrids present were confirmed to be F1s. This was further supported by morphology as no transgressive characters were detected. B. crispa was found to be the maternal parent in one population (BH), while in the second population (TJ), it was difficult to distinguish the hybridization direction due to shared haplotypes of cpDNA between B. alternifolia and B. crispa. Conclusions: These results provide evidence that the natural hybrids between B. alternifolia and B. crispa mainly comprise F1 hybrids, which have subsequently been given the name B. wardii. The F1 hybrids have also contributed to strong reproductive isolation between parental species.
Comparative transcriptomic analysis provides an insight into floral organ petaloid in lotus (Nelumbo nucifera)
Authors: Lin Zhongyuan , Cao Dingding , Damaris Rebecca Njeri , Yang Pingfang 
Published: July 2020

Abstract
Background: Lotus (Nelumbo nucifera) is a famous flower with high ornamental value. Flower color and flower morphology are two main factors for flower lotus breeding. Petaloid is a universal phenomenon in lotus flowers. However, the genetic regulation of floral organ petaloid in lotus still remains unclear. Result: In this study, transcriptomic analysis was performed among five organs, including petal, stamen petaloid, stamen, carpel petaloid, and carpel in lotus. Using WGCNA analysis, 37 candidate genes were found to be related to carpel petaloid. Additionally, one floral homeotic gene encoded MADS box transcription factor, AGAMOUS (AG), was identified as candidate gene for petaloid in lotus. Conclusion: The above results explored the candidate genes related to petaloid, setting a theoretical basis for the molecular regulation of petaloid.
Assessment of Indoor Microbiological Air Contamination in Research Facility at University of Malaysia
Authors: Mazlan Sytty Mazian , Hamzah Ainon , Noor Wan Syaidatul Aqma Wan Mohd , Abas Azlan 
Published: July 2020

Abstract
Indoor air quality is a concept that applies to the nature of the environment in and around buildings and facilities in which it contributes to the safety and security of those in the house. The aim of this study was to identify bacterial and fungal species present in the room, determine indoor air quality and investigate consumer views on indoor air quality in the Biology Building, Faculty of Sciences and Technology, National University of Malaysia. This study was conducted at the Biology Building at 8 selected sampling sites. Microbes were sampled using nutrient agar (bacteria) and potato dextrose agar (fungus). All samples of microbes were classified using two approaches; i) morphological examination and ii) biochemical reaction experiments. CO2, temperature and relative humidity are registered using the Direct Sense Monitoring Kit. A survey on customer satisfaction with indoor air quality in the building was carried out and evaluated in order to collect empirical details. This study found that the presence of Bacillus cereus, Bacillus laterosporus, Bacillus sphaericus, Micrococcus luteus, Staphylococcus aureus, Staphylococcus epidermis, Enterobacter cloacae, Pseudomonas fluarescens, Pseudomonas stuzeri and Aeromonas hydrophila bacterial. The fungi species are Aspergillus niger, Aspergillus nidulans, Penicillium digitatum and Fusarium dimerum. The result also shows that the carbon dioxide, temperature and relative humidity concentrations for most sampling stations comply with the DOSH standards. Moreover, almost all participants reported that their level of health and comfort while in the building is good, while the ventilation system of the building is at a comfortable level. Whereas, the degree of knowledge for most respondents on the value of indoor air quality is high. Two of the recommendations included in this study to enhance indoor air quality are to insure that the air conditioning device is correctly controlled and to raise understanding of the value of indoor air quality among staff and students in the Biology Building.
Negative correlation between concentration of iron oxides and particulate matter in atmospheric dust: case study at industrial site during smoggy period
Authors: Petrovsky Eduard , Kapicka Ales , Grison Hana , Kotlik Bohumil , Miturova Hana 
Published: July 2020

Abstract
Background: Environmental magnetism, focusing on ferrimagnetic iron oxides, provides useful additional information on pollution of different environments. Magnetic methods have been applied to studies of atmospheric dust, namely PM10 (particulate matter smaller than 10 micrometers) in, e.g., industrial or urban areas. Until now, positive correlation was reported between concentration of iron oxides (expressed in terms of either magnetic susceptibility, saturation remanent or saturation induced magnetization) and concentration of PM10 or smaller. Purpose of this study was to verify the relationship between iron oxides and PM at monitoring site close to source of emissions rich in iron oxides during period of smoggy conditions. Results: We examined 24-hours PM10 and PM1 samples, collected during 10 days of smoggy winter period at a site close to steel plant, which represents a significant source of atmospheric emissions in industrial region of Northern Moravia (Czech Republic), known for generally high degree of air pollution. Magnetic hysteresis loops were measured in order to obtain parameters reflecting the concentration and grain-size distribution of iron oxides. Our data show unexpected negative correlation between saturation magnetization (concentration of ferrimagnetic iron oxides) and both PM1 and PM10 concentrations, to the best of our knowledge the trend not being reported yet. Conclusions: Our finding may seemingly disqualify magnetic methods as useful proxy in air pollution studies. However, we suggest that this is an exceptional case, specific to this region and monitoring site, as well as to synoptic conditions during the smoggy period. Although the significant dust emissions are presumably rich in iron oxides, the overall air quality at the monitoring site is determined by the general environment, controlled by many other sources of different character in the region, and by the specific climatic conditions. Thus, the nearby steel plant, presumably emitting dust rich in ferrimagnetic iron oxides, dominates the deposited dust at the nearby monitoring site only during very few days of suitable weather (namely wind speed and direction).
Noninvasive Evaluation of Intracranial Pressure by Transcranial Doppler Ultrasound in Patients With Traumatic Brain Injury
Authors: Chang Tao , Yang Yanlong , Qian Zhen , Guo Qingbao , Li Lihong 
Published: July 2020

Abstract
Background
As a noninvasive monitoring measure, transcranial Doppler ultrasound (TCD) has been widely used to monitor the secondary brain injury in patients with traumatic brain injury (TBI). There are different physiological theories on the noninvasive assessment of intracranial pressure by TCD parameters, including ONSD and PI, which may cause that the change of ONSD and PI is not always synchronous with that of ICP. Therefore, the objective of this study was to investigate the relationship between PI or ONSD and ICP at different levels or in different periods after the operation, and the ability of prediction intracranial hypertension with these parameters in patients with TBI.
Methods
The clinical data of 68 patients with TBI were retrospectively analyzed. The statistical correlation analysis was performed to investigate the relationship between the PI or ONSD and ICP one week after the operation. Besides, the area under the curve (AUC) of ONSD or PI alone or a combination of them was calculated to determine the ability of intracranial hypertension.
Results
1. There was a correlation between ONSD and ICP ≥ 20 mmHg (r = 0.665, p < 0.001), ICP < 20 mmHg (r = 0.358, p = 0.006). The correlation still remained at ONSD ≥ 5 mm (r = 0.644, p < 0.001), but no correlation at ONSD < 5 mm (p = 0.137). 2. There was a strong correlation between PI and ICP at ICP of 15–20 mmHg (r = 0.705, p < 0.001), and ICP ≥ 20 mmHg (r = 0.716, p < 0.001). Nevertheless, it revealed a weak correlation at PI < 1.2 (r = 0.271, p = 0.021), PI ≥ 1.2 (r = 0.350, p = 0.020). In different period after the operation, there was a moderate correlation between ICP and PI on days 3, 4, and 5 (r = 0.508, p < 0.001), a strong correlation on days 6 and 7 after the operation (r = 0.645, p < 0.001). 3. For prediction intracranial hypertension with PI ≥ 1.2 or ONSD ≥ 5 mm alone or a combination of ONSD ≥ 5 mm and PI ≥ 1.2, the AUC value was 0.729 (p < 0.001), 0.900 (p < 0.001), and 0.943 (p < 0.001), respectively.
Conclusion
The correlation between the parameters of TCD, including ONSD and PI, and invasive ICP vary at different levels of ICP and in different periods in patients with TBI post-operation. It could also allow for a more accurate prediction of elevated intracranial pressure with a combination of ONSD ≥ 5 mm and PI ≥ 1.2.
Unfolding the Hidden Message of Anode-Free Lithium Metal Battery
Authors: Huang Chen-Jui , Thirumalraj Balamurugan , Tao Hsien-Chu , Shitaw Kassie Nigus , Hagos Tesfaye Teka , Beyene Tamene Tadesse , Kuo Li-Ming , Wang Chun-Chieh , Wu She-huang , Su Wei-Nien , Hwang Bing Joe 
Published: July 2020

Abstract
Lithium metal batteries (LMBs) have been revisited and gained great attention due to significantly mitigated formation of Li dendrite in the past decade. Recently, anode-free lithium metal batteries (AFLMBs) are proposed and have been studied intensively to potentially outperform LMBs due to higher energy density and reduced safety hazards since the absence of Li metal during the fabrication process of the cell. In general, researchers compare capacity retention, reversible capacity, or rate capability of the cells to study the electrochemical performance of batteries. However, evaluating the behavior of batteries from limited aspects would easily overlook other information hidden deep inside the meretricious results or even lead to misguided data interpretation. In this work, an integrated protocol combining different types of cell configuration is proposed and validated for the first time to unravel the concealed messages in LMBs and AFLMBs. Irreversible coulombic efficiency (irr-CE) from various contributions including reductive electrolyte decomposition, dead Li formation, 1st intrinsic irreversible capacity of a cathode, and the subsequent irreversible reactions at cathode containing oxidative electrolyte decomposition and cathode degradation upon cycling are successfully determined separately by the integrated protocol for the first time. The decrypted information obtained from the proposed protocol provides an insightful understanding of behaviors of LMBs and AFLMBs, which promotes their development for practical applications.