bluuebunny/crossref_metadata_2025_split huggingface dataset:
Foxi1inactivation rescues loss of principal cell fate selection inHes1-deficient kidneys but does not ensure maintenance of principal cell gene expression
Authors: Mukherjee Malini , DeRiso Jennifer , Janga Madhusudhana , Fogarty Eric , Surendran Kameswaran 
Published: November 2019

AbstractThe distal nephron and collecting duct segments of the mammalian kidney consist of intercalated cell types intermingled among principal cell types. Notch signaling ensures that a sufficient number of cells select a principal instead of an intercalated cell fate. However, the precise mechanisms by which Notch signaling patterns the distal nephron and collecting duct cell fates is unknown. Here we observed thatHes1, a direct target of Notch signaling pathway, is required within the mouse developing collecting ducts for repression ofFoxi1expression, an essential intercalated cell specific transcription factor. Interestingly, inactivation ofFoxi1inHes1-deficient collecting ducts rescues the deficiency in principal cell fate selection, overall urine concentrating deficiency, and reduces the occurrence of hydronephrosis. However,Foxi1inactivation does not rescue the reduction in expression of all principal cell genes in the Hes1-deficient kidney collecting duct cells that select the principal cell fate. Additionally, suppression of Notch/Hes1 signaling in mature principal cells reduces principal cell gene expression without activatingFoxi1. We conclude that Hes1 is a Notch signaling target that is essential for normal patterning of the collecting ducts with intermingled cell types by repressingFoxi1, and for maintenance of principal cell gene expression independent of repressingFoxi1.
Retinoic acid organizes the vagus motor topographic map via spatiotemporal regulation of Hgf/Met signaling
Authors: Isabella Adam J. , Barsh Gabrielle R. , Stonick Jason A. , Moens Cecilia B. 
Published: November 2019

SUMMARYThe topographic map, in which the positions of neuron cell bodies correspond with the positions of their synaptic targets, is a major organizational motif in the nervous system. To understand how topographic axon targeting is controlled during development, we examine the mechanism underlying topographic innervation of the pharyngeal arches by the vagus motor nerve in zebrafish. We reveal that Retinoic Acid organizes the topographic map by specifying anterior-posterior identity in post-mitotic vagus motor neurons. We then show that chemoattractant signaling between hepatocyte growth factor (Hgf) and the Met receptor is required for pharyngeal arch innervation by the vagus motor nerve. Finally, we find that Retinoic Acid controls the spatiotemporal dynamics of Hgf/Met signaling to coordinate axon targeting with the developmental progression of the pharyngeal arches and show that experimentally altering the timing of Hgf/Met signaling is sufficient to redirect axon targeting and disrupt the topographic map. These findings establish a new mechanism of topographic map development in which regulation of chemoattractant signaling in both space and time guides axon targeting.
A genome-wide almanac of co-essential modules assigns function to uncharacterized genes
Authors: Wainberg Michael , Kamber Roarke A. , Balsubramani Akshay , Meyers Robin M. , Sinnott-Armstrong Nasa , Hornburg Daniel , Jiang Lihua , Chan Joanne , Jian Ruiqi , Gu Mingxin , Shcherbina Anna , Dubreuil Michael M. , Spees Kaitlyn , Snyder Michael P. , Kundaje Anshul , Bassik Michael C. 
Published: November 2019

SUMMARYA central remaining question in the post-genomic era is how genes interact to form biological pathways. Measurements of gene dependency across hundreds of cell lines have been used to cluster genes into ‘co-essential’ pathways, but this approach has been limited by ubiquitous false positives. Here, we develop a statistical method that enables robust identification of gene co-essentiality and yields a genome-wide set of functional modules. This almanac recapitulates diverse pathways and protein complexes and predicts the functions of 102 uncharacterized genes. Validating top predictions, we show thatTMEM189encodes plasmanylethanolamine desaturase, the long-sought key enzyme for plasmalogen synthesis. We also show thatC15orf57binds the AP2 complex, localizes to clathrin-coated pits, and enables efficient transferrin uptake. Finally, we provide an interactive web tool for the community to explore the results (coessentiality.net). Our results establish co-essentiality profiling as a powerful resource for biological pathway identification and discovery of novel gene functions.
The α1 subunit of the Na,K-ATPase acts upstream of PI(4,5)P2 facilitating unconventional secretion of Fibroblast Growth Factor 2 from tumor cells
Authors: Legrand Cyril , Saleppico Roberto , Sticht Jana , Lolicato Fabio , Müller Hans-Michael , Wegehingel Sabine , Dimou Eleni , Steringer Julia P. , Ewers Helge , Vattulainen Ilpo , Freund Christian , Nickel Walter 
Published: November 2019

SummaryFibroblast Growth Factor 2 (FGF2) is a tumor cell survival factor that is exported from cells by an unconventional secretory pathway. This process is based on direct translocation of FGF2 across the plasma membrane. FGF2 membrane translocation depends on PI(4,5)P2-induced formation of membrane-inserted FGF2 oligomers followed by extracellular trapping of FGF2 at the outer leaflet mediated by cell surface heparan sulfate proteoglycans. Beyond the well-characterized core mechanism of FGF2 membrane translocation, the Na,K-ATPase has been proposed to play a so far unknown role in unconventional secretion of FGF2. Here, we define a direct physical interaction of FGF2 with a subdomain of the cytoplasmic part of the α1 subunit of the Na,K-ATPase. Employing NMR spectroscopy and molecular dynamics simulations, we identified two lysine residues on the molecular surface of FGF2 that are shown to be essential for its interaction with α1. In intact cells, the corresponding lysine-to-glutamate variants of FGF2 were characterized by inefficient secretion and reduced recruitment to the inner plasma membrane leaflet as shown by single molecule TIRF microscopy. Our findings suggest that α1 acts upstream of PI(4,5)P2 facilitating efficient membrane translocation of FGF2 to the cell surface of tumor cells.
Does directors’ experience positively moderate the impact of board busyness on firm efficiency? Evidence from Asia-Pacific
Authors: Tan Khar Mang , Bany-Ariffin A.N. , Kamarudin Fakarudin , Abdul Rahim Norhuda 
Published: November 2019

Purpose
The purpose of this paper is to examine the impact of board busyness on firm efficiency in the context of directors’ experience, specifically on directors’ experience that moderates the impact of board busyness on firm efficiency. Directors’ experience is examined by exploring both depth (board tenure) and breadth (number of former listed directorship) of experience.

Design/methodology/approach
This paper employs data envelopment analysis (DEA) to examine firm efficiency. Then, fixed effect panel regression analysis is applied to test the direct and moderating effect based on a sample of firms in the selected Asia-Pacific countries.

Findings
Significant positive evidence for the moderating effect of directors’ experience on the impact of board busyness on firm efficiency is documented.

Practical implications
Findings are essential for managers, country policymakers and potential investors as inputs to improve the current company practices, laws and policies through the notion that directors’ experience does enable the busy board to contribute to improved firm efficiency.

Originality/value
This paper contributes to the debated perspectives on board busyness by providing initial evidence that directors’ experience positively moderates the impact of board busyness on firm efficiency.
The importance of cross-border cooperation in the quality assurance of TNE
Authors: Trifiro Fabrizio 
Published: November 2019

Purpose
The purpose of this paper is to provide evidence on the importance of cross-border cooperation in the quality assurance of transnational education (TNE) by offering a comparative overview of how TNE is quality assured by both sending and receiving countries. Through this comparative analysis, it will be possible to appreciate the diversity of approaches to TNE. The paper also shows that for the foreseeable future, progress towards effective and effiicient quality assurance of TNE cannot rest on the hope of developing an internationally agreed framework, but it must rest on strengthening cooperation between quality assurance agencies operating within different national frameworks.

Design/methodology/approach
This paper looks at four key sending countries of TNE, the UK, Australia, the USA and Germany, considering the main drivers for out-going TNE, its main features and how it is quality assured. It then considers the same aspects from the perspective of receiving countries of TNE, looking at four key receiving locations, China, Dubai, Singapore and Hong Kong. It also offers an overview of some recent international initiatives aimed at fostering cross-border cooperation in the quality assurance of TNE.

Findings
In the backdrop of this overview of different approaches to TNE, this paper will conclude that for the foreseeable future it is not possible to develop an internationally agreed view and approach to TNE and its quality assurance. This conclusion allows the author to focus on the importance of cross-border cooperation amongst quality assurance agencies in sending and receiving countries for the effective and efficient quality assurance of TNE.

Originality/value
This is the first comparative study of different countries’ approaches to TNE and its quality assurance, taking into account both sending and receiving countries. It allows pointing to the key different features in different countries’ approaches and illustrates how these often relate to the underlining national strategic priorities and drivers for TNE (either in-bound or out-bound). It also allows the international community to realise that in the absence of a viable shared and agreed international framework for the regulation and quality assurance of TNE, it is of paramount importance to focus efforts on inter-agency cross-border cooperation in order to ensure that TNE continues to be of good quality and relevant to the respective communities.
From co-discovery to co-capture: co-innovation in themusic business
Authors: Saragih Harriman Samuel , Simatupang Togar , Sunitiyoso Yos 
Published: November 2019

Purpose
Previous work has asserted that the co-innovation process in the music business is composed of four stages, i.e. co-discovery, co-creation, co-delivery and co-capture. This study aims to re-examine and validate this proposed conceptualisation by gathering and interviewing additional respondents, specifically academics and professional event organisers, who were not formerly involved. By gaining more insight from different stakeholders, this study expects to gain more reliable results regarding the proposed concept derived from the previous study.

Design/methodology/approach
This study uses the case study method by carrying out qualitative interview data collection from 11 respondents. Narrative analysis is used in examining the findings. Pattern matching is used as the basis of the analysis using the proposed conceptualisation from co-discovery to co-capture of co-innovation as the rival analysis to the empirical findings discovered in this study. This paper also discusses how the validity and reliability of the qualitative analysis carried out are ensured.

Findings
This study supports the notion that the co-innovation process in the music industry follows the four stages of co-discovery, co-creation, co-delivery and co-capture. The respondents, from different professional backgrounds, interviewed in this study indicated and validated that the proposed framework aligns with their actual practices, expectations and realities, along with their specific roles in the music industry’s ecosystems.

Practical implications
The results of this study can be used as a reference in developing guidelines or policies for co-innovation practices in the music business, which previous studies have not explored, e.g. focusing only on preconditions for positive collaboration, open license and music for co-creation or discussions that are merely conceptual.

Originality/value
This study validates the co-innovation process in the music business proposed by the previous works, which integrates the value chain thinking concept within the analysis.
Sham project compliance behaviour
Authors: Darling Eric John , Whitty Stephen Jonathan 
Published: November 2019

PurposeThe purpose of this paper is to examine a case of sham compliance performance reporting through the lens of Goffman’s dramaturgy to reveal its dramaturgical structure. It makes a methodological contribution to comprehending “lived experience” accounts of project work, and adds knowledge concerning the behind-the-scenes motivators to sham behaviour in project work.Design/methodology/approachUsing an ethnographic lived experience account, an aspect of project work is reconceptualised as a collection of dramaturgical scenes. These scenes disclose issues beyond the bounds of the traditional project management discourse, and increase knowledge and appreciation of sham and performative behaviour in project work.FindingsSham progress reporting can emerge in an environment where senior management’s ignorance of project work creates unworkable binds for project staff. Moreover, the sham behaviour succeeds at its objective because senior management are vulnerable to false impressions. This situation raises ethical issues for those involved, and creates an overhead in dealing with the reality of project work.Research limitations/implicationsLimitations to this study are due to the inherent nature of the ethnographic method, where it is difficult to recruit willing participants, particularly in terms of sham behaviour cases. This study has implications for research on sham and performativity behaviour in project work, as studies can benefit from the dramaturgical analysis and Goffmanesque scene illustration techniques that help give focus to particular aspects of social performance, and remove complexity from the narrative.Practical implicationsThe research provides practitioners with a way of discussing superfluous compliance process using additional lived experience vocabulary. This could reduce the undue pressure to behave unethically, and reduce the burden to create the extra impression management work.Originality/valueThis study brings a voice to sham behaviour in project work. Continued ignorance of sham behaviour results in unnecessary work and unprofitable projects. Individuals could pay a price in terms of stress and well-being, not discussed.
Optimization of the simultaneous pickup and delivery vehicle routing problem based on carbon tax
Authors: Qin Gaoyuan , Tao Fengming , Li Lixia , Chen Zhenyu 
Published: November 2019

Purpose
In order to reduce logistics transportation costs and respond to low-carbon economy, the purpose of this paper is to study the more practical and common simultaneous pickup and delivery vehicle routing problem, which considers the carbon tax policy. A low-carbon simultaneous pickup and delivery vehicle routing problem model is constructed with the minimum total costs as the objective function.

Design/methodology/approach
This study develops a mathematical optimization model with the minimum total costs, including the carbon emissions costs as the objective function. An adaptive genetic hill-climbing algorithm is designed to solve the model.

Findings
First, the effectiveness of the algorithm is verified by numerical experiments. Second, the research results prove that carbon tax mechanism can effectively reduce carbon emissions within effective carbon tax interval. Finally, the research results also show that, under the carbon tax mechanism, the effect of vehicle speed on total costs will become more obvious with the increase of carbon tax.

Research limitations/implications
This paper only considers the weight of the cargo, but it does not consider the volume of the cargo.

Originality/value
Few studies focus on environmental issues in the simultaneous pickup and delivery problem. Thus, this paper constructs a green path optimization model, combining the carbon tax mechanism for the problem. This paper further analyzes the impact of carbon tax value on total costs and carbon emission; at the same time, the effect of vehicle speed on total cost is also analyzed.
The impact of collaboration network on new product development
Authors: Chen Peizhen 
Published: November 2019

PurposeResearchers agree that collaboration networks can be an important implement in a firm’s innovation process, but there is limited empirical evidence on actually how they facilitate the new product development (NPD). The paper aims to discuss these issues.Design/methodology/approachUsing longitudinal and multisource data on a sample of firms engaged in the Chinese automobile industry, the authors examine the structural properties of collaboration networks and their possible influences on firms’ NPD performance.FindingsThe results indicate that the structural features of the technology-based collaboration networks in the automobile industry have a low degree of collaborative integration and they influence firms’ NPD performance in diverse ways. The authors find that the direct ties, indirect ties and structural holes of the collaboration networks are all positively associated with firms’ number of new products. However, the authors have not found the evidence that the number of direct ties can moderate the relationship between the indirect ties and the NPD performance.Originality/valueFirst, previous researches concerning the network mainly focused on their influence on technology innovation, few scholars studied the relationship between collaboration network and NPD. Second, the data used in this paper are true and valid, they are all from relevant departments of the Chinese government. Third, the empirical research of new products in China’s manufacturing industry is relatively new.
A comparison of statistical and decision-making techniques in marketing mix evaluation
Authors: Shokrani Mahsa , Haghighi Mohammad , Paricheh Morteza , Shokrani Mina 
Published: November 2019

Purpose
Although customer satisfaction (CS) has been evaluated through using statistical and decision-making techniques so far, no research, to the best of the authors’ knowledge, has been conducted for implementing both groups simultaneously and clarifying the different or similar aspects of the results given by these techniques. The purpose of this paper is to compare the techniques and clarify these unknown aspects.

Design/methodology/approach
First, the effect of the elements related to service marketing mix on CS was examined by using structural equation model (SEM). Then, the statistical methods such as Friedman test (FT) and SEM, and decision-making technique such as Analytical Hierarchy Process (AHP) were systematically compared for prioritizing the elements of service marketing mix. The sample included 159 special customers of an anonymous bank in Bojnurd, Iran. The reliability of the questionnaire was confirmed by using Cronbach’s α (r=0.934). In addition, SPSS, Expert Choice and Smart PLS software were used.

Findings
Based on the results, FT and AHP method had exactly the same ranking for the elements of the marketing mix, as well as almost identical relative weights. The ranking included people, process, product, physical evidence, place, price and promotion, respectively, while the SEM technique had very different outcomes. Finally, none of the methods could assure the marketer to come to a reliable decision separately.

Originality/value
In this study, the authors’ contribution is the understanding of the role of an effective marketing mix evaluation technique selection on marketing strategy. Different techniques had different and in some cases even contradicting outcomes.
The prevalence of depression and post-traumatic stress disorder among internally displaced persons in Maiduguri, Nigeria
Authors: Aluh Deborah Oyine , Okoro Roland Nnaemeka , Zimboh Adamu 
Published: November 2019

PurposeThe purpose of this paper is to assess the prevalence of depression and post-traumatic stress disorder (PTSD) among internally displaced persons (IDPs) in Maiduguri.Design/methodology/approachThe study was a cross-sectional study that took place among the six IDP camps located in Maiduguri metropolis in Borno State. A non-randomized technique was used to sample 1,200 respondents. Face-to-face interviews with selected members of households were carried out confidentially. The study used the Patient Health Questionnaire (PHQ-9) and Impact of Event Scale-6 which were translated to Kanuri. Descriptive and inferential statistics were employed using SPSS version 21.FindingsThe response rate was 100 percent. In total, 96.1 percent (1,153) of the respondents were depressed, while 78 percent (936) of the respondents were symptomatic for PTSD. The prevalence rate of comorbid PTSD with depression was 68.1 percent (817). About one-third of the respondents had moderately severe depression (29.6 percent,n=355) while about one in ten of them were severely depressed (11.3 percent,n=136). The odds of being depressed was 3.308 higher in people aged 51–60 years compared to people between 18 and 20 years. Significant predictors of depression in the sampled population were screening positive for PTSD and being unemployed.Practical implicationsThe high prevalence of depression and PTSD among the sampled population calls for structured interventions to deal with mental health problems. The study findings suggest the need for more research (preferably qualitative) on the mental health issues in this population.Originality/valueThis study contributes to the sparse available literature on the mental health of IDPs in Nigeria.