bluuebunny/crossref_metadata_2025_split huggingface dataset:
Glial cell line‐derived neurotrophic factor alters lipid composition and protein distribution in MPP+‐injured differentiated SH‐SY5Y cells
Authors: Mu Peipei , Liu Yuting , Jiang Shimin , Gao Jin , Sun Shen , Li Li , Gao Dianshuai 
Published: May 2020

AbstractParkinson's disease (PD) is a neurodegenerative disease characterized by progressive loss of dopaminergic neurons in the substantia nigra and striatum. Glial cell line‐derived neurotrophic factor (GDNF) can effectively promote the differentiation and survival of many types of neurons, especially dopaminergic neurons, suggesting it could be a treatment for PD. Lipid rafts are highly dynamic cell membrane domains that contain numerous signal protein receptors, providing an important platform for signal transduction. Compelling evidence indicates that alterations in lipid rafts are associated with PD, and some studies have reported that GDNF can regulate the expression of caveolin‐1, a lipid raft‐marker protein. However, the precise effects of GDNF on lipid rafts remain unknown. We developed a cellular PD model, purified detergent‐resistant membranes (membrane rafts), and performed proteomic and lipid metabolomics analyses to examine changes in lipid rafts after GDNF treatment. The results showed considerable protein and lipid alterations in response to GDNF, especially altered levels of dopamine‐β‐hydroxylase, heat shock 70 kDa protein, neural cell adhesion molecule, cytoskeletal proteins, and long‐chain polysaturated/unsaturated fatty acids. These findings reveal a new avenue to explore the relationships between GDNF, lipid rafts, and PD and support the hypothesis that GDNF may be a useful treatment for PD.
p120‐catenin suppresses proliferation and tumor growth of oral squamous cell carcinoma via inhibiting nuclear phospholipase C‐γ1 signaling
Authors: Li Lusha , Ji Shangli , Shrestha Chandrama , Jiang Yi , Liao Liyan , Xu Feng , Liu Zhenming , Bikle Daniel D. , Xie Zhongjian 
Published: May 2020

Abstractp120‐catenin (p120) serves as a stabilizer of the calcium‐dependent cadherin‐catenin complex and loss of p120 expression has been observed in several types of human cancers. The p120‐dependent E‐cadherin‐β‐catenin complex has been shown to mediate calcium‐induced keratinocyte differentiation via inducing activation of plasma membrane phospholipase C‐γ1 (PLC‐γ1). On the other hand, PLC‐γ1 has been shown to interact with phosphatidylinositol 3‐kinase enhancer in the nucleus and plays a critical role in epidermal growth factor‐induced proliferation of oral squamous cell carcinoma (OSCC) cells. To determine whether p120 suppresses OSCC proliferation and tumor growth via inhibiting PLC‐γ1, we examined effects of p120 knockdown or p120 and PLC‐γ1 double knockdown on proliferation of cultured OSCC cells and tumor growth in xenograft OSCC in mice. The results showed that knockdown of p120 reduced levels of PLC‐γ1 in the plasma membrane and increased levels of PLC‐γ1 and its signaling in the nucleus in OSCC cells and OSCC cell proliferation as well as xenograft OSCC tumor growth. However, double knockdown of p120 and PLC‐γ1 or knockdown of PLC‐γ1 alone did not have any effect. Immunohistochemical analysis of OSCC tissue from patients showed a lower expression level of p120 and a higher expression level of PLC‐γ1 compared with that of adjacent noncancerous tissue. These data indicate that p120 suppresses OSCC cell proliferation and tumor growth by inhibiting signaling mediated by nuclear PLC‐γ1.
Silica‐immobilized acid ionic liquid: An efficient catalyst for pentanal self‐condensation
Authors: Wang Wenhao , Wang Di , Yang Qiusheng , An Hualiang , Zhao Xinqiang , Wang Yanji 
Published: May 2020

AbstractBACKGROUNDPentanal self‐condensation to 2‐propyl‐2‐heptenal is an important process of carbon chain extension for the industrial manufacture of 2‐propylheptanol, which is an important plasticizer alcohol. The existing industrial processes all use liquid alkali catalysts, so they suffer from problems such as difficult recovery and high costs for treatment of alkali‐containing wastewater. In the present work, three immobilized acid ionic liquids were prepared separately with SiO2, MCM‐41, and SBA‐15 as support for pentanal self‐condensation.RESULTSThe structures of the as‐prepared immobilized acid ionic liquids were confirmed with the help of FT‐IR, TG, SEM‐EDS, and N2 adsorption–desorption techniques. The characterization and activity evaluation results indicate that there are positive correlations among the number of hydroxyl groups on the support surface, ionic liquid loading, and the catalytic activity. Among the three immobilized acid ionic liquids, silica‐immobilized acid ionic liquids (SiO2‐IL‐SO3H) showed an extraordinary catalytic performance for pentanal self‐condensation because of its higher ionic liquid loading. The yield and selectivity of 2‐propyl‐2‐heptenal could respectively reach 73.9% and 91.5% under suitable reaction conditions and a high turnover number (TON) of 179.1 was obtained.CONCLUSIONPyridine adsorption infrared spectroscopy and density functional theory (DFT) simulation were employed to investigate the structure and acidity of SiO2‐IL‐SO3H. The optimized structures were obtained, and the calculation results indicate that the acidity of SiO2‐IL‐SO3H is higher than the corresponding homogeneous ionic liquid system, which may be the main reason for its excellent catalytic performance and catalytic efficiency. © 2020 Society of Chemical Industry
Liquefaction of corn husks and properties of biodegradable biopolyol blends
Authors: Briones Rodrigo , Rodriguez Jesús , Labidi Jalel , Cunningham Eoin , Martin Peter 
Published: May 2020

AbstractBACKGROUNDA valorization route of corn husks from agrarian practices was performed by liquefaction using glycerol as liquefaction solvent to obtain biopolyols used as bioadditives in starch blends with the aim to find a useful industrial application in polymer processing.RESULTSLow‐molecular‐weight liquefied products obtained from a practically total conversion reaction can be used for composite formulations (OH number, 310 mg KOH g−1; viscosity, 3.4 Pa s; molecular weight, 549 g mol−1). Concurrently, starch thermoplastic blends using various proportions of biopolyol (40, 30 and 20 wt%) as plasticizer were produced with maximum torque and plasticization energy ranging from 3.4 to 15.1 Nm and from 1.7 to 9.1 Nm min−1. The starch/biopolyol (70/30) sheets obtained by thermopressing showed properties similar to those of controls (starch/glycerol: 70/30), such as sensitivity to environment, retrodegradation, biodegradability and density; however, mechanical properties exhibited better performance compared to controls (Young’s modulus, 14 MPa; strain at break, 33%; tensile strength, 1.2 MPa), which indicates a material with major mechanical balance.CONCLUSIONSThe adequacy in the conversion of corn husks into biopolyols that could be used as improved biobased plasticizers to obtain biodegradable blends is revealed. Hence, this study demonstrates that liquefied corn husk residues are sustainable resources with suitable properties for polymer processing, which can be applied in bioplastics and be considered as a value‐added feature. © 2020 Society of Chemical Industry
Point‐of‐care ultrasound in the diagnosis of neonatal cerebral sinovenous thrombosis
Authors: Oulego‐Erroz Ignacio , Ocaña‐Alcober Carlos , Jiménez‐González Aquilina 
Published: May 2020

AbstractCerebral sinovenous thrombosis (CSVT) mostly affects sick neonates in the neonatal intensive care unit (NICU) with predisposing or underlying conditions. The clinical presentation is nonspecific which often leads to a delayed or missed diagnosis. Point‐of‐care ultrasound (POCUS) use in the NICU is rapidly increasing. One of the main uses of neonatologist‐performed POCUS is cranial ultrasound which permits diagnosis and monitoring of neurological disease at the bedside. We present the case of a neonate with a complex clinical situation where cranial POCUS permitted a prompt diagnosis and treatment of severe CSVT by imaging the transverse sinuses through the mastoid fontanelle.
Peroxisome proliferator‐activated receptor activity correlates with poor survival in patients resected for hepatocellular carcinoma
Authors: Pommergaard Hans‐Christian , Preuss Hasselby Jane , Linno Willemoe Gro , Ralbovska Adela , Arendtsen Rostved Andreas , Rasmussen Allan , Aagaard Schultz Nicolai , Hillingsø Jens , Nørgaard Larsen Peter , Kugler Jan‐Michael 
Published: May 2020

AbstractBackground/PurposeFew clinically useful biomarkers are known to predict prognosis in patients with hepatocellular carcinoma (HCC). The aim of this study was to investigate the correlation between PPAR activity and ALDH7A1 expression and their prognostic significance using RNA sequencing in patients undergoing liver resection for HCC.MethodsWe included patients undergoing liver resection for HCC at a tertiary referral center for hepato‐pancreato‐biliary surgery between May 2014 and January 2018. PPAR activity and ALDH7A1 expression were evaluated by RNA sequencing and correlated with overall survival, recurrence and histological features.ResultsWe included 52 patients with a median follow‐up of 20.9 months, predominantly males (88.5%) with a single tumor (84.6%) in a non‐cirrhotic liver (73.1%). Three‐year overall survival was 48.6% in patients with a specific PPAR target gene expression profile (cancer cluster 3) compared with 81.7% in controls (P = .04, Log‐rank test). This remained significant (odds ratio 14.02, 95% confidence interval 1.92‐102.22, P = .009) when adjusted for age, cirrhosis, microvascular invasion, number of tumors and free resection margins. ALDH7A1 expression was not correlated with PPAR or any outcomes.ConclusionPPAR activity in a subset of tumor samples was associated with reduced overall survival indicating that PPAR may be a valuable prognostic biomarker.
Development of a sensitive and specific nanoparticle‐assisted PCR assay for detecting HPV‐16 and HPV‐18 DNA
Authors: Ma Xingjie , Li Yucheng , Liu Ranran , Wei Wenping , Ding Changping 
Published: May 2020

AbstractCarcinoma precursor lesion caused by persistent infection of human papillomavirus (HPV) types 16 and 18 is known as a principal inducer of cervical cancer. Therefore, rapid and effective detection of HPV‐16 and HPV‐18 infection at early stage is an important strategy for preventing such disease. In this study, a novel duplex nanoparticle‐assisted polymerase chain reaction (nanoPCR) assay was developed to detect both of the two genotypes simultaneously. Two pairs of primers for nanoPCR were designed based on the conserved region within the early 6 (E6) gene of HPV‐16 and HPV‐18, respectively. After optimizing reaction conditions, the nanoPCR assay displayed 10‐fold more sensitive than that of conventional PCR and showed high specificity. The detection limit of nanoPCR was 1.7 × 101 copies/μL for HPV‐16, 1.2 × 102 copies/μL for HPV‐18, and no cross‐reaction was detected after using other viruses or HPV subtypes as templates. Of 209 clinical samples collected from patients, as also confirmed by sequencing, the nanoPCR method gave consistent results with conventional PCR assay: 7 positives for HPV‐16, 4 positives for HPV‐18, and no co‐infection. Here is the first report to introduce a reproducible nanoPCR assay for detecting HPV DNA with high sensitivity and specificity, which may point out a useful diagnostic tool for potential clinical application.
A model of supply‐chain decisions for resource sharing with an application to ventilator allocation to combat COVID‐19
Authors: Mehrotra Sanjay , Rahimian Hamed , Barah Masoud , Luo Fengqiao , Schantz Karolina 
Published: May 2020

AbstractWe present a stochastic optimization model for allocating and sharing a critical resource in the case of a pandemic. The demand for different entities peaks at different times, and an initial inventory for a central agency are to be allocated. The entities (states) may share the critical resource with a different state under a risk‐averse condition. The model is applied to study the allocation of ventilator inventory in the COVID‐19 pandemic by FEMA to different U.S. states. Findings suggest that if less than 60% of the ventilator inventory is available for non‐COVID‐19 patients, FEMA's stockpile of 20 000 ventilators (as of March 23, 2020) would be nearly adequate to meet the projected needs in slightly above average demand scenarios. However, when more than 75% of the available ventilator inventory must be reserved for non‐COVID‐19 patients, various degrees of shortfall are expected. In a severe case, where the demand is concentrated in the top‐most quartile of the forecast confidence interval and states are not willing to share their stockpile of ventilators, the total shortfall over the planning horizon (until May 31, 2020) is about 232 000 ventilator days, with a peak shortfall of 17 200 ventilators on April 19, 2020. Results are also reported for a worst‐case where the demand is at the upper limit of the 95% confidence interval. An important finding of this study is that a central agency (FEMA) can act as a coordinator for sharing critical resources that are in short supply over time to add efficiency in the system. Moreover, through properly managing risk‐aversion of different entities (states) additional efficiency can be gained. An additional implication is that ramping up production early in the planning cycle allows to reduce shortfall significantly. An optimal timing of this production ramp‐up consideration can be based on a cost‐benefit analysis.
Real‐time interaction of virtual and physical objects in mixed reality applications
Authors: Badías Alberto , González David , Alfaro Icíar , Chinesta Francisco , Cueto Elías 
Published: May 2020

SummaryWe present a real‐time method for computing the mechanical interaction between real and virtual objects in an augmented reality environment. Using model order reduction methods we are able to estimate the physical behavior of deformable objects in real time, with the precision of a high‐fidelity solver but working at the speed of a video sequence. We merge tools of machine learning, computer vision, and computer graphics in a single application to describe the behavior of deformable virtual objects allowing the user to interact with them in a natural way. Three examples are provided to test the performance of the method.
ProNetView‐ccRCC: A Web‐Based Portal to Interactively Explore Clear Cell Renal Cell Carcinoma Proteogenomics Networks
Authors: Kalayci Selim , Petralia Francesca , Wang Pei , Gümüş Zeynep H. 
Published: May 2020

AbstractTo better understand the molecular basis of cancer, the NCI's Clinical Proteomics Tumor Analysis Consortium (CPTAC) has been performing comprehensive large‐scale proteogenomic characterizations of multiple cancer types. Gene and protein regulatory networks are subsequently being derived based on these proteogenomic profiles, which serve as tools to gain systems‐level understanding of the molecular regulatory factories underlying these diseases. On the other hand, it remains a challenge to effectively visualize and navigate the resulting network models, which capture higher order structures in the proteogenomic profiles. There is a pressing need to have a new open community resource tool for intuitive visual exploration, interpretation, and communication of these gene/protein regulatory networks by the cancer research community. In this work, ProNetView‐ccRCC (http://ccrcc.cptac-network-view.org/), an interactive web‐based network exploration portal for investigating phosphopeptide co‐expression network inferred based on the CPTAC clear cell renal cell carcinoma (ccRCC) phosphoproteomics data is introduced. ProNetView‐ccRCC enables quick, user‐intuitive visual interactions with the ccRCC tumor phosphoprotein co‐expression network comprised of 3614 genes, as well as 30 functional pathway‐enriched network modules. Users can interact with the network portal and can conveniently query for association between abundance of each phosphopeptide in the network and clinical variables such as tumor grade.
Local integration and co‐evolution of internationalizing Chinese firms
Authors: Li Wei , Hendrischke Hans 
Published: May 2020

AbstractThis study investigates the integration of internationalizing Chinese firms into local host markets. We explore the market‐driven investment of a new wave of Chinese private and local state‐owned firms in Australia since 2012, which has replaced the initial large‐scale investment in resources by central state‐owned enterprises. Using an in‐depth analysis of nine Chinese firms operating in various sectors of the Australian market, we argue that market integration, adaptation, and bilateral institution‐building through co‐evolution and empowerment of local subsidiaries of Chinese multinational enterprises results in entrepreneurial autonomy and characterizes a new generation of Chinese investors. We propose that Chinese multinational subsidiaries have transferred domestic practices to the Australian market and have reconfigured domestic and host market resources to gain a competitive advantage in their original investment industry and new industries. Our study advances middle‐range theory building and provides a practical understanding of the strengths and weaknesses of Chinese investors, their potential to disrupt local markets, and their responsiveness to market‐oriented institutional guidance. The results of this study suggest that the bilateral institution‐building and resource reconfiguration capabilities of Chinese enterprises can be transferred to other developed and developing markets, including Belt and Road Initiative countries.
The role of bladder instillation in the treatment of bladder pain syndrome: Is intravesical treatment an effective option for patients with bladder pain as well as LUTS?
Authors: Digesu Giuseppe Alessandro , Tailor Visha , Bhide Alka A. , Khullar Vik 
Published: May 2020

AbstractThe aetiology of bladder pain syndrome/interstitial cystitis is still unknown. Numerous mechanisms have been proposed and treatments targeting various aspects of these are used. This review looks at the existing evidence on bladder instillations and whether they could be used in the treatment of lower urinary tract symptoms as well.
Comparison of Clinical Outcomes Between Surgical Gastrostomy and Percutaneous Endoscopic Gastrostomy with Introducer Technique in Patients with Upper Aerodigestive Malignancies: A Single‐Center Analysis
Authors: Chang Arunchai , Watcharamon Chomduan , Rattanasupa Attapon , Thongsonkleeb Kittikarn , Chowdok Bunlue , Khaimook Araya , Ovartlarnporn Bancha , Prachayakul Varayu 
Published: May 2020

AbstractBackgroundConventional percutaneous endoscopic gastrostomy (PEG) in patients with upper aerodigestive malignancies inevitably carries a risk of stomal metastasis that could be avoided by the direct insertion of the gastrostomy tube through the abdomen. This study compared the efficacy and safety between surgical gastrostomy (SG) and Introducer PEG in patients with upper aerodigestive malignancies.MethodsWe retrospectively reviewed patients with upper aerodigestive malignancies undergoing SG or Introducer PEG. Procedure data, postprocedural clinical outcomes and 30‐day mortality were assessed.ResultsIn total, 99 patients were feasible to analysis: 53 were in the SG group, and 46 were in the Introducer PEG group. The SG group had a higher incidence of (in‐hospital) major complications [28.3% VS 4.3%, p = 0.002], a longer procedure duration [52.02 ± 21.30 VS 21.46 ± 8.22 min, p < 0.001], higher pain scores at 24 h [median (interquartile range, IQR); 5(3–8) VS 1(0–5), p < 0.001] and longer length of hospitalization (LOH) [median (IQR); 5(4–6) days VS 3(2–4) days, p < 0.001)]. In‐hospital (3.8% VS 0%, p = 0.493) and 30‐day mortality (17.0% VS 13.0%, p = 0.586) were not different between the two groups. In univariate analysis, high BMI, anemia (hemoglobin <11 g/dL), normal nutritional status (serum albumin >3 g/dL) and procedural type were found to be predicting factors for complications. Procedural type (Introducer PEG) was an independent factor for major complications in multivariate analysis [OR = 0.12, 95% CI 0.02–0.61, p= 0.011].ConclusionsIn patients with upper aerodigestive malignancies, Introducer PEG was associated with lower rate of (in‐hospital) major complications, faster operative time, lower pain scores and shorter LOH.Clinical trials registry numberTCTR20181220004
Development and Pilot Test of a Virtual Reality Respiratory Biofeedback Approach
Authors: Blum Johannes , Rockstroh Christoph , Göritz Anja S. 
Published: May 2020

AbstractBreathing exercises with biofeedback have benefits over breathing exercises without biofeedback. However, the traditional measurement of respiratory signals that is required as part of feeding back the breath incurs high cost and effort. We propose a novel virtual reality (VR) based approach to respiratory biofeedback that utilizes the positionally tracked hand controllers integrated into modern VR systems to capture and feedback the respiration-induced abdominal movements. In a randomized controlled laboratory study, we investigated the feasibility and efficacy of the developed biofeedback algorithm. In total, 72 participants performed a short breathing exercise in VR with or without respiratory biofeedback. The feedback integration resulted in a satisfactory user experience, a heightened breath awareness, a greater focus on slow diaphragmatic breathing and an increased respiratory sinus arrhythmia. This evidences that the novel biofeedback approach is low-cost, unobtrusive, usable and effective in increasing breath awareness and promoting slow diaphragmatic breathing in the context of VR-based breathing exercises. Future studies need to investigate the broader applicability and long-term effects.
Entrepreneurial orientation and new venture resource acquisition: why context matters
Authors: Yin Miaomiao , Hughes Mathew , Hu Qilin 
Published: May 2020

AbstractResource acquisition is vital for new venture survival and growth. However, surprisingly little is known about how the entrepreneurial orientation (EO) of the new venture affects its resource acquisition. Drawing on the resource-based view of the firm, we articulate a theory and treatment of EO that address this oversight and remedy for the routine absence of context among studies of EO. Accounting for the simultaneous effect of environmental dynamism and an opportunistic orientation (OO), a tendency among Chinese new ventures to imitate technology and profit through market information asymmetry, as important contextual variables reflecting the Chinese business context, we provide insights on the contingency effects of contextual variables. Results from a quantitative study of 361 Chinese new ventures show that EO positively influences resource acquisition. However, this relationship is context sensitive. In a low dynamic environment, OO negatively moderates this relationship. However, in a highly dynamic environment, OO exhibits no effect on the relationship between EO and new venture resource acquisition. Our results contribute to a resource-based theory of EO and reveal its context sensitivity. Our study is a step in moving the scholarship of EO forward and away from the performance debate towards greater predictive accuracy of EO and its systems of effects.