bluuebunny/crossref_metadata_2025_split huggingface dataset:
STUDY PROTOCOL The prevalence and risk factors of work-related musculoskeletal disorders among adults in Ethiopia: a study protocol for extending a systematic review with meta-analysis of observational studies
Authors: Gebreyesus Tsiwaye , Nigussie Kalkidan , Getnet Moges Gashaw , Janakiraman Balamurugan 
Published: May 2020

Abstract
Background: Work-related musculoskeletal disorders impose a significant and most often underappreciated burden to the individual, nation, healthcare system, and society as a whole. To the best of our knowledge, there is a lack of reliable estimates on the prevalence of work-related musculoskeletal disorders in Ethiopia. The objective of this study will be to assess the existing literature on the prevalence rates and determinant factors of work-related musculoskeletal disorders in Ethiopia.Method: We will search PubMed/MEDLINE, Embase, SCOPUS, PsycINFO, PEDro and Ebsco (from January 2000 onwards). Grey literature will be identified through searching Google Scholar and dissertation databases. Observational studies reporting on the prevalence of work-related musculoskeletal disorders among adult Ethiopians will be included. The primary outcome will be the prevalence of work-related musculoskeletal disorders. Secondary outcomes will be the prevalence of any risk factors in association with work-related musculoskeletal disorders. Two reviewers will independently screen all citations, full-text articles, and abstract data. The study methodological quality (or bias) will be appraised using an appropriate tool. If feasible, we will conduct random effects meta-analysis of observational data. Subgroup analyses will be conducted to explore the potential sources of heterogeneity (e.g. gender, sample size, type of occupation).Publication bias and heterogeneity between the included studies will also be assessed and reported.Discussion: This systematic review will provide a synthesis of the literature on the prevalence of work-related musculoskeletal disorders and their risk factors in Ethiopia. The results of this review could help the policymakers in occupational health and health care sectors in identifying priority areas for interventions in work-related musculoskeletal disorders and will also serve as a baseline for the decision-making processes of musculoskeletal health promotion, work exposure implementations, and prevention programs in workplaces. Systematic review registration: PROSPERO CRD42020164240
Therapeutic abortion in Iran: an epidemiologic study of legal abortion in 2 years
Authors: Mahdavi Seyed Amirhosein , Jafari Asieh , Azimi Khadijeh , Dehghanizadeh Nikoo , Bazegar Abdolrazagh 
Published: May 2020

Abstract
Objectives: Unsafe abortion is one of the most important causes of death and disability among mothers in countries where abortion is illegal. These conditions have changed since then. The present study has investigated the cases who were referred to the legal medicine organization to receive abortion permission. This country level secondary patient data analysis, investigated all the cases who were referred to the legal medicine centers of Iran for abortion permission during 2015 to 2017.Results: From 21477 applicants, 15617 (72.71%) received permission including 14367 (91.99%) for fetal abnormalities and 1250 (8.01%) for maternal diseases. The most common fetal abnormalities/disorders were nervous system malformations (26.4%), chromosomal abnormalities (18.4%) and of maternal diseases were circulatory system diseases (43.9%), neoplasms (13.4%) and genitourinary system diseases (9.9%). The most common reasons for not permission were lack of supplementary documents to prove (38.8%), not competency with the criteria (33.9%), and gestational age of more than 19 weeks (25.8%).
Correlation of Clinicopathological Features and IL6 Expression in Tumor Budding of Colon Adenocarcinoma
Authors: Sato Koichi , Uehara Takeshi , Iwaya Mai , Nakajima Tomoyuki , Tobe Yosuke , Miyagawa Yusuke , Ota Hiroyoshi 
Published: May 2020

Abstract
Background: Interleukin-6 (IL6) is one of the main cytokines produced by cancer-associated fibroblasts (CAFs). IL6 is linked with cancer progression and poor prognosis by activating cancer cells and modifying the cancer microenvironment. However, little is known about the expression of IL6 in tumor budding (TB) and its association with TB in colon adenocarcinoma (CA). Methods: The clinicopathological and prognostic significance of IL6 in TB was examined using a tissue microarray consisting of 36 patient samples of TB in CA. IL6 mRNA was detected by RNAscope kit. Patients were stratified into negative and positive IL6 expression groups.Results: IL6 expression was overwhelmingly observed in CAFs but was negligible in cancer cells. In the IL6-positive group in CAFs, TB grade was higher than in the IL6-negative group (P=0.0161). There was a significant difference in overall survival (OS) between CA cases in the IL6-positive group and the IL6-negative group (log rank test, P=0.0367). Cox proportional hazard regression model revealed that the IL6-negative group (OR = 0.25; 95% CI: 0.05–0.96; P=0.0440) had better OS for CA than the IL6-positive group. Conclusions: TB may be affected by IL6 expression, and IL6 expression in CAFs at TB may make IL6 an important prognostic marker.
Characteristics of Endocrine Metabolism in AL Amyloidosis Patients
Authors: Cao Changchun , Pan Binbin , Cao Feifei , Wan Xin 
Published: May 2020

Abstract
Background To explore characteristics of thyroid gland and glucose metabolism in light chain (AL) amyloidosis patients.Methods A total of 20 amyloidosis patients were recruited. Thyroid function and biochemical indices were tested. Intravenous glucose tolerance test was performed in all patients. Thyroid ultrasound was also determined.Results 1.There was 7 patients with hypothyroidism (35%) and 4 patients with subclinical hypothyroidism (20%). Additionally, a total of 6 patients manifested as euthyroid sick syndrome (30%); 2. A number of 15 patients showed thyroid nodule (75%); 3. The fast blood glucose was 4.85±0.8 mmol/l. The level of blood glucose after intravenous glucose tolerance test showed 8.03±1.88 mmol/l at 30 minutes and 7.1±3.22 mmol/l at 120 minutes. 4. The blood insulin levels in fasting, 30 minutes and 120 minutes were 5.2±2.59 mIU/L, 308.3±199.4 mIU/Land 245.66±176.44 mIU/L. Further, the values of C-peptide showed 2.47±1.43 ng/ml, 6.74±3.16 ng/ml and 8.98±4.83 ng/ml. Homeostasis model assessment of insulin resistance and Homeostasis model assessment of β cells of the patients were 1.2±0.62 and 88.45±46.17, respectively. There were 2 patients diagnosed with diabetes and 1 patient with impaired glucose tolerance.Conclusions AL amyloidosis patients revealed high prevalence of hypothyroidism, subclinical hypothyroidism and euthyroid sick syndrome. However, glucose metabolism showed no influence in amyloidosis patients.
Pistils may be required for anthocyanin synthesis —— the whole-transcriptome analysis of mutant and normal capitula of Chrysanthemum morifolium
Authors: Liu Hua , Luo Chang , Chen Dongliang , Wang Yaqin , Guo Shuang , Chen Xiaoxi , Bai Jingyi , Li Mingyuan , Huang Xinlei , Cheng Xi , Huang Conglin 
Published: May 2020

Abstract
Background Chrysanthemum morifolium is one of the most economically important and popular floricultural crops in Asteraceae. Chrysanthemums have many different flower colors and shapes. However, the molecular mechanism controlling the development of chrysanthemum floral colors and shapes is still an enigma. We obtained a cut chrysanthemum variety with mutant capitula in which the ray florets became green and the inside pistils became vegetative buds, while normal capitula have many rounds of purple ray florets and few disc florets. ﻿Results We conducted whole-transcriptome analysis of differentially expressed genes (DEGs) between the mutant and normal capitula using third-generation and second-generation sequencing techniques. We identified DEGs between the mutant and normal capitula to reveal important regulators underlying their differential development. Regulatory genes involved in the photoperiod pathway and the control of floral organ identification as well as important functional genes in the anthocyanin synthesis pathway were also identified. Therefore, a list of candidate genes for studying flower development and anthocyanin synthesis in chrysanthemums was generated. Qualitative analysis of pigments in the florets of normal and mutant capitula revealed anthocyanins were synthesized and accumulated in the florets of normal capitula, but not in the florets of mutant capitula. It was indicated that pistils may be required for anthocyanin synthesis in chrysanthemums. Conclusions These results will help to elucidate the molecular mechanisms of floral organ development and will contribute to the development of techniques for studying flower shape and color regulation to promote breeding in chrysanthemum.
Incomplete Staging in Ovarian Cancer
Authors: Beltman Jogchum , Laven P , Bense JE , Aa Maaike van der , Vos MC , Boll D , Arts HGJ , Reesink N , Trimbos JB , Kruitwagen RFPM 
Published: May 2020

Abstract
Objective Incomplete surgical staging of patients with early stage epithelial ovarian cancer (EOC) has been reported in up to 85% of cases, when based on the International Federation of Obstetrics and Gynaecology (FIGO) staging procedure. The aim of the present retrospective study was to clarify the reasons for incomplete staging.Methods The PRISMA (Prevention Recovery Information System for Monitoring and Analysis) technique was used to evaluate cases with FIGO I-IIa EOC based on incomplete staging from five gynecologic oncologic center hospitals in the Netherlands in the period 2010-2014.Results Fifty cases with an incomplete surgical staging of EOC according to national guidelines were included. The most common reasons for incomplete staging were insufficient random biopsies of the peritoneum (n=34, 68%), and less than ten lymph nodes being resected and/or found at pathology (n=16, 32%). The most mentioned reasons for not performing biopsies were forgetting to do so, and believing that after careful inspection and palpation, taking biopsies is irrelevant and/or already are being taken while performing a hysterectomy (cul-de-sac, bladder). The value of contralateral pelvic lymph node dissection in case of a unilateral ovarian malignancy was also doubted, influencing the number of lymph nodes resected.Conclusion The most important reasons for incomplete staging in EOC are, besides omitting elements by accident, questioning the importance of obligatory elements of the staging procedure. A structured list of staging steps during surgery and more evidence based consensus concerning these obligatory elements might increase the number of complete staging procedures in EOC.
Mitochondrial DNAs provide insight into trypanosome phylogeny and molecular evolution
Authors: Kay Christopher , Williams Tom A , Gibson Wendy 
Published: May 2020

Abstract
Background: Trypanosomes are single-celled eukaryotic parasites characterised by the unique biology of their mitochondrial DNA (mtDNA). African livestock trypanosomes impose a major burden on agriculture across sub-Saharan Africa, but are poorly understood compared to those that cause sleeping sickness and Chagas disease in humans. Here we explore the potential of trypanosome mtDNA to study the evolutionary history of trypanosomes and the molecular evolution of their mtDNAs.Results: We used long-read sequencing to completely assemble mtDNAs from four previously uncharacterized African trypanosomes, and leveraged these assemblies to scaffold and assemble a further 103 trypanosome mtDNAs from published short-read data. While synteny was largely conserved, there were repeated, independent losses of Complex I genes. Comparison of edited and non-edited genes revealed the impact of RNA editing on nucleotide composition, with non-edited genes approaching the limits of GC loss. African tsetse-transmitted trypanosomes showed high levels of RNA editing compared to other trypanosomes. Whole mtDNA coding regions were used to construct time-resolved phylogenetic trees, revealing deep divergence events among isolates of the pathogens Trypanosoma brucei and T. congolense .Conclusions: Our mtDNA data represents a new resource for experimental and evolutionary analyses of trypanosome phylogeny, molecular evolution and function. Molecular clock analyses yielded a timescale for trypanosome evolution congruent with major biogeographical events in Africa and revealed the recent emergence of Trypanosoma brucei gambiense and T. equiperdum , major human and animal pathogens.
Evaluation of Maternal Death Surveillance and Response System in Dewachefa Woreda, Oromia Zone, Amhara Region, Ethiopia, 2018
Authors: Tariku Mengistie 
Published: May 2020

Abstract
Background

Maternal death surveillance and response (MDSR) is the “litmus test” of the health system that provides evidence for accomplishment, links activities to results, makes maternal death visible at all levels, informs communities & health workers, increases country ownership of data, provides information in real time and allows improvement towards catching all maternal mortalities. The aim of study was to evaluate maternal death surveillance and response system in Dewachefa.
Methods

A cross sectional study design was conducted in two health centers, five health post, district health office and from these facilities 32 health workers were included. Data were collected through focal person, health worker and health extension worker interview by using checklist. Collected data were entered into Epi data version 3.1. These data were exported to statistical package for social science for analysis. Analyzed data were presented in the form of text, table and figures.
Result

The average completeness of weekly report form of the district was 77.4%. Twenty-eight (87.5%) of the health worker had not got Maternal death surveillance and response (MDSR) training. All visited health facilities and Woredas focal person were trained. The system had under notification of maternal death from the community, poor involvement of health facility staff, and discordance of data between public health emergency management, and maternal and newborn health unit report. Establish rapid response team that includes maternal and child health staff’s maternal death review committees in all health facilities.
Aortic Thrombus in patients with Severe Covid-19. Review of three cases
Authors: Carranza Maria , Salazar Danilo , Troya Jesús , Alcazar Roberto , Peña Cristina , Muñoz Nuria 
Published: May 2020

Abstract
Coronavirus disease 2019 (COVID-19) is suspected to predispose to both venous and arterial thromboembolism, in the context of an exaggerated immune response to the virus, especially in severe patients. Even though aortic thrombi are a rare entity, the new COVID-19 establishes the need to include them in the diagnosis, especially in patients with severe disease and no clinical improvement. Herein, we describe a series of three cases of aortic thrombi diagnosed by computerized tomography (CT) angiography in patients with confirmed SARS CoV-2 infection.
Comparative Transcriptomics of Metabolites in Wild and Cultivated Pinellia ternata
Authors: Wang Jue , Zhu Yufeng , Lv Xiangyu , Xiong Yue , Lu Jiawei , Jin Chao , Zhang Jian , Liu Shijia 
Published: May 2020

Abstract
Background: As a traditional Chinese medicine(TCM) used to treat cough, Pinellia ternata (PT) has been utilized for thousands of years. Studies focused on the genetic makeup underlying the differences between wild and cultivated PT are rare, while wild herb is quite effective and indispensable in the clinical use of TCM. There is still rare researches related to the cause of differences between wild and cultivated PT. With the application of transcriptome technology in the field of traditional Chinese medicine, High-throughput RNA sequencing (RNA-seq) could be employed to find out how environment affect the accumulation of active constituent in medicinal herbs.Result: Overall, 133 503 unigenes were assembled, and 1329 genes were differentially expressed in the cultivated PT compared to the wild PT. Of those, 70 DEGs (differentially expressed genes) were significantl[1]y downregulated and 9 were upregulated in the cultivated PT compared to the wild PT after filtering out genes involved in the biosynthesis and metabolism of amino acids, glutathione, phenylpropane and other constituents. These genes were assumed to be of special interest with respect to the better efficacy of the wild PT. Meanwhile, RT-qPCR was utilized to analysis the expression patterns of 9 selected genes and HPLC were applied to quantify the content of adenine and guanosine in PT which verified the RNA-Seq result.Conclusion: 79 DEGs were found in wild and cultivated PT, which played an important role in the synthesis and metabolism of the active ingredients of PT. And the expression of DEGs is higer in wild PT, indicated the importance of wild medicinal herb resources. The experiment produced robust resources for future investigations on the regulation of active ingredients in PT and Araceae and the promotion of the cultivation and clinical use of PT.
Clinical Analysis of Classification for Tibial Plateau Fractures and Popliteal Artery Injury
Authors: Yao Yilun , Wu Xiaoshu , Wu Lei , Yang Lei , Jiang Chunzhi , Yang Wengbo 
Published: May 2020

Abstract
Objective To explore the association between the classification for tibial plateau fractures (TPF) and the popliteal artery injury (PAI).Methods 22 TPF patients accompanied by PAI who were treated from May 2012 to July 2019 were retrospectively analyzed. There were 19 males and 3 females with an average age of 49.43 years. The Schatzker classification and three-column classification were performed for TPF. The severity of extremity injury was evaluated using the mangled extremity severity score (MESS). Except 3 patients treated with amputation, the remaining patients underwent surgical repair of popliteal artery and fracture external fixation. The efficacy was evaluated using the Rasmussen score for tibial head fractures.Results There were 10 cases of Schatzker type IV fractures, 1 case of type V fractures and 11 cases of type VI fractures. Based on the three-column classification, the posterior column was involved in 22 cases, 2 columns in 15 cases and 3 columns in 6 cases. The MESS was 6-10 points, with an average of 7.59 points. Except 1 case directly receiving amputation, 3 cases of segment P1 injury was observed via preoperative DSA + intraoperative exploration, while segment P2 in 6 cases and segment P3 in 12 cases. Popliteal artery was found completely ruptured in 11 cases, partially ruptured in 1 case, and severely contused with thrombosis in 10 cases. The Rasmussen score was given to 19 patients at the last follow-up,except for the cases undergoing amputation. The efficacy was excellent in 8 cases, good in 7 cases, moderate in 3 cases and poor in 1 case.Conclusion: In patients with complex TPF, the risk of PAI becomes higher with the increase of Schatzker classification level. Knee CT scan is helpful in determining the severity of fractures and evaluating PAI. Based on the three-column classification, PAI should be suspected when the fractures involve the medial and posterior column.PAI is mainly in the segment P3, and artery rupture or severe contusion with extensive thrombosis may occur.The postoperative knee function of patients with complex TPF can be well restored by early active surgical repair of popliteal artery.
A new transpedicular lag-screw fixation for treatment of unstable Hangman’s fracture: a minimum 2-year follow-up study
Authors: Liu Yijie , Zhu Yi , Li Xuefeng , Chen Jie , Yang Sen , Yang Huilin , Jiang Weimin 
Published: May 2020

Abstract
Background
A new C2 transpedicular lag-screw designed by our team has been used in human cadaver spines for biomechanical testing, and the results showed that the biomechanical properties of the new C2 transpedicular lag-screw were better than ordinary screws. The objective of this study is to analyze the clinical efficacy and safety of the new C2 transpedicular lag-screw fixation for treatment of an unstable Hangman’s fracture.
Methods
From March 2013 to June 2017, 25 patients who had unstable Hangman’s fractures were operated on with a new C2 transpedicular lag-screw fixation. The patients included 18 males and 7 females whose ages ranged from 31–62 years (average 45.4 ± 9.3 years). The cause of the injury was a traffic accident in 17 patients and a fall from height in 8 patients. Other associated lesions included rupture of the spleen (1 patient) and rib fractures (2 patients). According to the Levine-Edwards classification, 17 patients were Type II and 8 patients were Type IIA, and according to the Frankel Neurological Performance scale, 8 cases and 17 cases were graded as spinal cord injury D and E, respectively. Of the cases, 23 cases received bilateral screw fixation and 2 cases had unilateral screw fixation because another pedicle was chipped. The whole procedure was accomplished with monitoring by“C”-arm fluoroscopy.
Results
The mean follow-up time was (36 ± 12) months and ranged from 24 to 60 months. No obvious symptomatic or radiologic postoperative complications were found during the follow-up period. 6 cases restored from D to E while 2 cases remained D according to American Spinal Injury Association (ASIA) grade. Osseous union was achieved in all cases, and the range of cervical motion recovered to normal level up to the last follow-up.
Conclusions
The primary clinical and radiographic efficacies of a new C2 transpedicular lag-screw fixation for treatment of an unstable Hangman’s fracture were satisfactory. This approach could be considered a simple, effective, reliable and economic surgical method for managing unstable Hangman’s fractures.