bluuebunny/crossref_metadata_2025_split huggingface dataset:
A novel circular RNA circ-LRIG3 facilitates the malignant progression of hepatocellular carcinoma by modulating the EZH2/STAT3 signaling
Authors: Sun Suofeng , Gao Jing , Zhou Shen , Li Yuan , Wang Yu , Jin Li , Li Jian , Liu Bowei , Zhang Bingyong , Han Shuangyin , Ding Hui , Li Xiuling 
Published: November 2020

Abstract
Background: Circular RNA (circRNA) is emerging as an important player in human diseases, especially cancer. In our previous study, we identified a series of deregulated circRNAs in hepatocellular carcinoma (HCC) by performing circRNA microarray expression profile. Here, we aimed to explore the role of circ-LRIG3 (hsa_circ_0027345) in HCC.Methods: qRT-PCR and western blot were used to asses gene and protein expression, respectively. CCK-8, EdU and Transwell assays were used to detect cell proliferation, migration and invasion. GSEA software was applied to analyze the pathway related to circ-LRIG3. Co-IP, RIP and ChIP assays were used to identify the positive feedback axis of circ-LRIG3/EZH2/STAT3. Animal study was carried to test the role of circ-LRIG3 in vivo.Results: Circ-LRIG3 was notably upregulated in HCC and promoted HCC cell proliferation, migration, invasion and reduced apoptosis. Circ-LRIG3 formed a ternary complex with EZH2 and STAT3, facilitating EZH2-induced STAT3 methylation and subsequent phosphorylation, resulting in the activation of STAT3 signaling. In turn, activated STAT3 could directly bind to circ-LRIG3 promoter to increase circ-LRIG3 transcription activity, thus forming a positive feedback loop. The animal models showed that exogenous expression of circ-LRIG3 enhanced tumorigenicity and metastasis in vivo, whereas these effects were blocked after treatment with C188-9, a specific STAT3 small-molecule inhibitor. Clinically, high circ-LRIG3 was closely linked with aggressive clinicopathological features and was identified as an independent risk prognostic factor of overall survival. Importantly, plasma circ-LRIG3 was found to be a highly sensitive and specific non-invasive diagnostic indicator for HCC.Conclusions: Our study reveals the carcinogenic role of circ-LRIG3 in HCC, which may provide a new therapeutic target for HCC patients.
Loss of MIG-6 results in endometrial progesterone resistance via ERBB2
Authors: Yoo Jung-Yoon , Kim Tae Hoon , Shin Jung-Ho , Marquardt Ryan , Mueller Ulrich , Fazleabas Asgerally , Young Steven , Lessey Bruce , Yoon Ho-Geun , Jeong Jae-Wook 
Published: November 2020

Abstract
Female subfertility is highly associated with endometriosis. Although the exact etiology of endometriosis-related infertility remains to be determined, endometrial progesterone resistance has recently been suggested as a crucial element in the development of endometrial diseases. Here, we report that MIG-6, a progesterone-induced gene, is downregulated in the endometrium of infertile women with endometriosis and in a non-human primate model of endometriosis. In an endometriosis mouse model with a fluorescent reporter used to identify lesions, an increase of endometriosis development and implantation failure were observed in mice with Mig-6 deficient endometrium compared to controls. MIG-6 is known to inhibit ERBB2, which we found overexpressed in the endometrium from uterine-specific Mig-6 knock-out mice (Pgrcre/+Mig-6f/f; Mig-6d/d). To investigate the effect of ERBB2 targeting on endometrial progesterone resistance, fertility, and endometriosis, we introduced Erbb2 ablation in Mig-6d/d mice (Mig-6d/dErbb2d/d mice). The additional knockout of Erbb2 rescued all phenotypes seen in Mig-6d/d mice including endometrial progesterone resistance, infertility, and endometriosis lesion development. Transcriptomic analysis showed that genes differentially expressed in Mig-6d/d mice reverted to their normal expression amounts in Mig-6d/dErbb2d/d mice. Together, our results demonstrate that MIG-6-induced ERBB2 overexpression causes endometrial progesterone resistance and a nonreceptive endometrium in endometriosis-related infertility and that ERBB2 targeting reverses these effects.
Exploring the impact of soil and water salinity on dietary behavior and health risk of coastal communities in Bangladesh
Authors: Shuvo Suvasish Das , Zahid Md. Ashrafuzzaman , Rahman Mohammad Mahfuzur , Parvin Rashida 
Published: November 2020

Abstract
The purpose of this study was to evaluate the impact of soil and water salinity on dietary behavior and health risk in the coastal people of Bangladesh. This study was conducted among 240 respondents in rural coastal sub-districts in Khulna and Patuakhali of Bangladesh using a simple random sampling technique. To evaluate the association between health risk and salinity exposure categories, a multinomial logit regression analysis was conducted and statistical signiﬁcance was declared at p ≤ 0.05. A significantly higher amount of salinity (NaCl) level was found in radish, potato, bean, bitter gourd, rice, shallow tube-well, and pond water from Patuakhali than Khulna. Males and those aged 36–50 years (RRR:1.89, SE:0.58) and 51–65 years (RRR:4.51, SE:1.81) were associated with hypertension compared with the females (RRR:0.57, SE:0.18) and age group 20–35 years. Consumption of shallow tube-well water (RRR:3.12, SE:1.46), salt content rice (RRR:1.36, SE:0.50), salt content vegetables (RRR:1.09, SE:0.09), salt content fish (RRR:2.77, SE:0.47), and intake of table salt (RRR:1.05, SE:0.03) were signiﬁcantly associated with risk factors of hypertension (p &lt; 0.01). A sustainable policy for salt reduction through dietary interventions along with the promotion of low saline foods and drinking water must be a priority with special emphasis on coastal areas.
Study on the dynamic characteristics of shallow groundwater level under the influence of climate change and human activities in Cangzhou, China
Authors: Yan Baizhong , Li Xiaomeng , Hou Junliang , Bi Pan , Sun Fengbo 
Published: November 2020

Abstract
Based on the monitoring data of groundwater level, mining data and meteorological data from 1986 to 2015 in Cangzhou City, the change characteristics of depth of groundwater level were studied. On this basis, the nonparametric statistical test method (Mann–Kendall method) was used to study the trend of depth of groundwater level. Besides In addition, the principal component analysis method and grey correlation method were used to study groundwater influence factors. Finally, the multivariable time series model was used to predict the depth of groundwater level. The results showed that in the past 30 years, influenced by human activities and meteorological changes, the groundwater flow field in 1986–2015 was generally move from southwest to northeast, the depth of groundwater level was increasing from the initial 3.26 to 4.06 m, with an annual increase rate of 0.027 m/a; the contribution rate of exploitation factor, precipitation factor, evaporation factor were 40, 20 and 40%; the fitting figure of the observed values and the predicted values were very good, with an average relative error of 7.73%. According to the prediction schemes, when the evaporation increases by 5%, and the agricultural exploitation decreases by 5%, the depth of groundwater will reach 3.39 m; when the evaporation increases by 10%, and the agricultural exploitation decreases by 10%, the depth of groundwater will reach 3.32 m. This study had important reference significance for regional groundwater treatment and rational utilization.
How anatase TiO2 with {101} {001} and {100} surfaces affect the photooxidation process of roxithromycin
Authors: Wei Zhigang , Chen Shiyun , Fang Yangfei , Wang Zhenrui , Liang Kai , Amakanjaha Anselem C. , Zhong Yuanhui 
Published: November 2020

Abstract
TiO2 crystals are widely used in photocatalytic processes due to their low cost and fabulous catalytic performance. As described in our previous study, three types of TiO2 with the main surfaces of {101}, {001} and {100} were synthesized. In this study, the three types of TiO2 are used to investigate roxithromycin (ROX) photocatalytic degradation kinetics and the pH effect. For photocatalytic degradation, the obtained data have shown that the overall order of optimal degradation is shown as {101} &gt; {001} &gt; {100}. The photooxidation kinetics for {101} facet conforms to first-order kinetics at from pH 5 to pH 10, and most of the photooxidation kinetics for {001} and {100} facets are fitted well with the zero-order and second-order kinetics, respectively. The pH effects are varied to the three types of TiO2, of which {101} has the best degradation effect at pH values 4, 7 and 8, while {001} works best at pH 5 or pH 6, and {100} has a relatively obvious effect at pH 4 and pH 9. The relation between adsorption and oxidation has been tested and proved that the strong adsorption corresponds to the fast oxidation.
A Urinary Metabolomics Analysis Based on UPLC-MS and Effects of Moxibustion in APP/PS1 Mice
Authors: He Rui , Liu Juntian , Huang Chang , Liu Jinyi , Cui Herong , Zhao Baixiao 
Published: November 2020

Background:Alzheimer's disease (AD) is a common neurodegenerative disorder with the symptoms of cognitive impairment and decreased learning and memory abilities. Metabolomics can reflect the related functional status and physiological and pathological changes in the process of AD. Moxibustion is a unique method in traditional Chinese medicine, which has been used in the treatment and prevention of diseases for thousands of years.Methods:A total of 32 APP/PS1 mice were randomly divided into the model group, moxibustion group, moxa smoke group and smoke-free moxibustion group (n=8/group), using the random number table method, while eight C57BL/6 mice were used as the control group. The five groups were measured for 20 min/day, 6 days/week, for 4 weeks. After 4 weeks’ experiment, all the mice were placed in metabolic cages to collect urine continuously for 24 hours, for UPLC-MS analysis.Results:Principal component analysis (PCA) was used to identify the different metabolites among the five groups, and partial least squares discriminant analysis (PLS-DA) was performed to reveal the effects on the metabolic variance. Sixteen potential biomarkers were identified among the five groups, primarily related to amino acid metabolism, starch metabolism, sucrose metabolism, interconversion of pentose and glucuronate, and aminoacyl biosynthesis. There were 17 differences in the potential metabolites between the control and model groups, involving the metabolism of amino acid, purine, pyrimidine, nicotinic acid and nicotinamide, and biosynthesis of pantothenate and coenzyme A. Fifteen potential biomarkers were identified between the model and moxibustion groups, related to starch metabolism, sucrose metabolism, interconversion of pentose and glucuronate, glyoxylate, dicarboxylate anions and some amino acid metabolism.Conclusion:Moxibustion can regulate the metabolism of substance and energy by improving the synthesis and decomposition of carbohydrates and amino acids in APP/PS1 transgenic AD model mice.
APOE Genotypes and Brain Imaging Classes in Normal Cognition, Mild Cognitive Impairment, and Alzheimer’s Disease: A Longitudinal Study
Authors: Camarda Cecilia , Torelli Paola , Pipia Carmela , Sottile Gianluca , Cilluffo Giovanna , Camarda Rosolino 
Published: November 2020

Objective:
To evaluate in 419 stroke-free cognitively normal subjects (CN) aged 45-82 years
covering during a long prospective study (11.54 ± 1.47 years) the preclinical to dementia spectrum: 1)
the distribution of small vessel disease (V) and brain atrophy (A) aggregated as following: V−/A−,
V−/A+, V+/A−, V+/A+; 2) the relationship of these imaging classes with individual apolipoprotein E
(APOE) genotypes; 3) the risk of progression to Alzheimer Disease (AD) of the individual APOE genotypes.

Methods:
Participants underwent one baseline (t0), and 4 clinical and neuropsychological assessments
(t1,t2,t3, and t4). Brain MRI was performed in all subjects at t0, t2, t3 and t4.. White matter hyperintensities
were assessed through two visual rating scales. Lacunes were also rated. Subcortical and global
brain atrophy were determined through the bicaudate ratio and the lateral ventricle to brain ratio, respectively.
APOE genotypes were determined at t0 in all subjects. Cox proportional hazard model was used
to evaluate the risk of progression to AD.

Results:
The imaging class of mixed type was very common in AD, and in non amnestic mild cognitive
impaired APOE ε4 non carriers. In these subjects, frontal and parieto-occipital regions were most affected
by small vessel disease.

Conclusion:
Our findings suggest that the APOE ε3 allele is probably linked to the brain vascular pathology.
Targeting Pathological Amyloid Aggregates with Conformation-Sensitive Antibodies
Authors: Bigi Alessandra , Loffredo Gilda , Cascella Roberta , Cecchi Cristina 
Published: November 2020

Background:
The pathogenesis of Alzheimer's disease (AD) is not directly caused by the
presence of senile plaques but rather by the detrimental effects exerted on neuronal cells by toxic soluble
oligomers. Such species are formed early during the aggregation process of the Aβ1-42 peptide or can be
released from mature fibrils. Nowadays, efficient tools for an early diagnosis, as well as pharmaceutical
treatments targeting the harmful agents in samples of AD patients, are still missing.

Objective:
By integrating in vitro immunochemical assay with in vivo neuronal models of toxicity, we
aim to understand and target the principles that drive toxicity in AD.

Methods:
We evaluated the specificity and sensitivity of A11 and OC conformational antibodies to target
a range of pathologically relevant amyloid conformers and rescue their cytotoxic effects in neuronal
culture models using a number of cellular readouts.

Results:
We demonstrated the peculiar ability of conformational antibodies to label pathologically relevant
Aβ1-42 oligomers and fibrils and to prevent their detrimental effects on neuronal cells.

Conclusion:
Our results substantially improve our knowledge on the role of toxic assemblies in neurodegenerative
diseases, thus suggesting new and more effective diagnostic and therapeutic tools for
AD.
An Overview on COVID-19 Pandemic: from Discovery to Treatment
Authors: Panati Kalpana , Tatireddygari Venkatramana Reddy Arva , Narala Venkata Ramireddy 
Published: November 2020

:
Recently, novel coronavirus infection (COVID-19) emerged in Wuhan, China has been
declared as pandemic by WHO. Until now, no evidence is documented regarding its wild animal
reservoir or intermediary host, but, human-to-human transmission, asymptomatic carriers were
very much observed. The number of confirmed cases and death toll have been increased almost all
over the world indicating its potential threat to public health. Though the phylogenetic analysis
shows some similarity of SARS-CoV2 to bat betacoronaviruses, it exhibited significant variation in
S1 domain of spike protein from bat-derived viruses. S1 domain plays an important role in receptor
binding and it can be a target for the development of therapeutics and vaccines. In this review, we
have discussed the updates on transmission, diagnosis, genome analysis and comparison, treatment
options and clinical trials of COVID-19.
Evaluation of a Nutraceutical Product with Probiotics, Vitamin D, Plus Banaba Leaf Extracts (Lagerstroemia speciosa) in Glycemic Control
Authors: Signorini Luca , Ballini Andrea , Arrigoni Roberto , De Leonardis Francesco , Saini Rajiv , Cantore Stefania , De Vito Danila , Coscia Maria Franca , Dipalma Gianna , Santacroce Luigi , Inchingolo Francesco 
Published: November 2020

Background:
Dysbiosis is related to changes in the composition and behaviour of intestinal
microbiota, which may contribute to an age-related decline in metabolic and immune system
functioning (immune-senescence).

Objective:
The microbiota-targeted dietary and probiotic interventions have been shown to favorably
affect the host health by an enhancement of antioxidant activity, improving immune homeostasis,
suppression of chronic inflammation, regulation of metabolism and prevention of insulin resistance.

Results:
In our study, the use of specific probiotics strains improved the serum concentration of glycemic
markers, thereby promoting better overall health.

Conclusion:
Probiotics may help correct defects in the gut microbial environment improving metabolic
parameters, such as blood sugar levels, glycosylated hemoglobin and a decrease in body weight.
Influence of long irregularities of the longitudinal profile on traffic safety under conditions of intensification of the transportation process
Authors: Pevzner V. O. , Chechel’nitskiy A. I. , Shapet’ko K. V. , Sidorova E. A. , Slastenin A. Yu. 
Published: November 2020

The key directions of intensification of the transportation process are described and their influence on the infrastructure is indicated. The concept of long irregularities of the longitudinal profile is defined and a method is proposed that allows one to study the geometric parameters and the growth rate of long irregularities. An assessment of the influence of long irregularities on energy consumption during the traction of trains is given.In the article, much attention is paid to the influence of long irregularities of the longitudinal profile on traffic safety. The article presents the results of the state analysis of the railway track section, where there was a derailment of the freight cars. The results of calculations of the level of forces arising in the automatic coupling and the coefficients of the vertical dynamics of the first stage of suspension for cars in a train when moving uphill along long irregularities are presented. Based on the results of the research, the main conclusions are formulated and the direction of further research is proposed.
Scientific priorities for the use of alternative energy sources in railway transport
Authors: Kosarev A. B. , Rebrov I. A. , Naumenko S. N. , Barch A. V. 
Published: November 2020

Alternative energy sources, mostly related to “green” energy, are one of the promising trends in the development of energy generation, including for the global railway sector. This is due, firstly, to its desire to preserve its environmental advantages by reducing carbon dioxide emissions into the environment and increasing energy efficiency, and secondly, to a reduction in the use of such sources as oil and coal as fuel resources in the coming decades.In a world where 95 % of motorized vehicles use oil as fuel, the greenhouse gas emissions per passenger-kilometer for rail transport are five times less than for road transport and 20 times less than for air transport. Therefore, one of the key tasks in the development of railway transport is the creation of high-speed highways that will serve as an alternative to road and air communications over short distances.In addition to the well-known alternative energy technologies, the railway transport traditionally conducts scientific research to improve energy-saving technologies, which include the use of excess recuperation energy when rolling stock braking.Also, projects for the use of renewable energy sources — heat pump installations — have proved their effectiveness in railway transport: heating switches from the ground, use of heat from compressors of hill complexes to heat electrical centralization posts and heat transformers of traction substations for its own needs.The role of alternative energy sources in railway transport is constantly growing. The efficiency of using alternative energy sources at the Russian Railways is largely determined by a scientific approach that allows, based on own experience or experience in introducing innovations in related industries, to determine the place, time and degree of their implementation, taking into account technical and technological features of production processes inherent in a particular structural unit companies. Among the innovative technical solutions in railway transport, the most priority ones are solutions aimed at the development of high-speed lines, recuperation systems, as well as heat pump technologies.
Some aspects of the use of natural gas motor fuel in diesel locomotives
Authors: Kossov E. E. , Asabin V. V. , Silyuta A. G. , Zhuravlev A. N. , Kossova L. E. 
Published: November 2020

Due to the increase in the cost of diesel fuel, much attention is paid to the use of alternative types of fuel on diesel locomotives. Variants of using fuel obtained from coal, plants, gas fields and hydrogen are considered. Natural gas is the cheapest and most accessible today. The use of specially designed gas-piston engines on diesel locomotives, operating when the gas-air mixture is ignited from an external source, is the most attractive option. However, this approach has certain disadvantages:• it is necessary to create a new engine, since the modernization of existing engines requires serious structural changes;• gas piston engine operates essentially according to the Otto cycle and has lower efficiency and power indicators as compared to a diesel engine;• when modernizing existing diesel locomotives, switching to the Otto cycle excludes the possibility of using diesel fuel.Conversion of diesel locomotives to gas fuel must be carried out using the gas-diesel cycle. This approach is most acceptable for the modernization of diesel locomotives of the existing fleet, since it preserves the thermal performance of the engine and makes it possible to transfer diesel locomotives back to operation on diesel fuel. The main obstacle to the transfer of diesel locomotives to the gas-diesel cycle is the low degree of replacement of diesel fuel with gas. This circumstance is determined by the significant difficulties in ensuring the operation of the engine in the gas-diesel cycle at low loads and idling. It is necessary to ensure a stable supply of ignition fuel in these modes and guaranteed ignition of the gas-air mixture from it. The solution to this problem is ensured by maintaining a given stoichiometric ratio in the gas-air mixture and a temperature sufficient to ignite the ignition portion of the fuel.The main way to regulate the stoichiometric ratio is to reduce the amount of air entering the cylinders by throttling it at the engine inlet. This article discusses the methodology for calculating the performance of the engine when throttling the air inlet.