bluuebunny/crossref_metadata_2025_split huggingface dataset:
UPLC-Q-TOF/MS based Untargeted Metabolite and Lipid Analysis on Premature Ovarian Insufficiency Plasma Samples
Authors: Taşcı Yasemin , Fındık Rahime Bedir , Pekcan Meryem Kuru , Kaplan Ozan , Celebier Mustafa 
Published: January 2020

Background:
Metabolomics is one of the main areas to understand cellular process at molecular
level by analyzing metabolites. In recent years metabolomics has emerged as a key tool to understand
molecular basis of diseases, to find diagnostic and prognostic biomarkers and develop new
treatment opportunities and drug molecules.

Objective:
In this study, untargeted metabolite and lipid analysis were performed to identify potential
biomarkers on premature ovarian insufficiency plasma samples. 43 POI subject plasma samples were
compared with 32 healthy subject plasma samples.

Methods:
Plasma samples were pooled and extracted using chloroform:methanol:water (3:3:1 v/v/v)
mixture. Agilent 6530 LC/MS Q-TOF instrument equipped with ESI source was used for analysis. A
C18 column (Agilent Zorbax 1.8 μM, 50 x 2.1 mm) was used for separation of the metabolites and lipids.
XCMS, an “R software” based freeware program, was used for peak picking, grouping and comparing
the findings. Isotopologue Parameter Optimization (IPO) software was used to optimize XCMS parameters.
The analytical methodology and data mining process were validated according to the literature.

Results:
83 metabolite peaks and 213 lipid peaks were found to be in semi-quantitatively and statistically
different (fold change >1.5, p <0.05) between the POI plasma samples and control subjects.

Conclusion:
According to the results, two groups were successfully separated through principal component
analysis. Among the peaks, phenyl alanine, decanoyl-L-carnitine, 1-palmitoyl lysophosphatidylcholine
and PC(O-16:0/2:0) were identified through auto MS/MS and matched with human metabolome
database and proposed as plasma biomarker for POI and monitoring the patients in treatment period.
Cytotoxic Sesquiterpenes from Sonchus oleraceus
Authors: Zhang Hongying , Wang Yiding , Wang Shaojiu , Wang Qian , Wang Tianjing , Zhu Jie , Liu Bing 
Published: January 2020

Background:
Sonchus oleraceus is a large and widespread plant in the world. It is edible to humans
as a leaf vegetable and is also used as a folklore medicinal herb in the treatment of infections and inflammatory
disease, but limited research on its chemical constituents has been done.

Objective:
To isolate and identify the bioactive ingredients from S. oleraceus.

Methods:
20kg of S. oleraceus was extracted twice with 75% alcohol. The concentrated extract was suspended
in H2O and partitioned with petroleum ether, dichloromethane, ethyl acetate and n-butanol, respectively. The
ethyl acetate phase was subjected to repeated normal chromatography on a silica gel column chromatography
and eluted with a gradient of CH2Cl2-MeOH to give 12 crude fractions. Fraction 6 was subjected to ODS silica
gel column chromatography, Sephadex LH-20 and HPLC to yield 1 and 2. Cell viability of 1 and 2 on A549,
H292 and Caco2 cell lines were assayed by MTT method. Apoptosis analysis and apoptosis related proteins
were detected subsequently.

Results:
Two new sesquiterpenes were isolated from S. oleraceus and identified by NMR spectra and HR-ESIMS.
1 selectively suppressed the viability of A549 and H292 cells with IC50 values of 14.2, and 19.5μM respectively,
while possessing no cytotoxicity against Caco2 cells (IC50 > 100μM). 2 did not exhibit cytotoxicity
against A549, H292 and Caco2 cells (IC50 > 100μM). 1 significantly decreased the density of live cells and
could cause cell apoptosis at 10 and 20μM in a dose-dependent manner. After treatment of 1 for 24h, the level of
cleaved caspase-3 was increased accompanied by the reduction in procaspase-3 expression, and the downregulation
of Bcl-2 was associated with the enhancement of Bax expression. 1 could lead to the up-regulation of
cytochrome c and activation of caspase-9.

Conclusion:
1 and 2 are new sesquiterpenes from S. oleraceus. 1 could induce apoptosis in A549 and H292
cells through Bax/caspase-9 pathway.
Torsional Vibration Suppression of Electric Vehicle Power Transmission System Based on Parameter Optimization and Fuzzy Control
Authors: Chen Guoqiang , Yang Zhifei 
Published: January 2020

Background:
The torsional vibration of the power transmission system has heavy effect on the
ride comfort and safety of the vehicle, which has attracted plenty of research. Therefore, aiming at the torsional
vibration problem of the electric vehicle power transmission system, enough study on how to suppress
the torsional vibration is clearly of great benefit.

Objective:
The goal of the work is to explore the new method to suppress the torsional vibration. The main
contribution is that the parameter of the power transmission system is optimized and the adaptive fuzzy PID
controller is proposed to be utilized and optimized to suppress the torsional vibration.

Methods:
An optimization objective function including the angular acceleration of the motor shaft, the decelerator
and differential assembly and the half shaft is established as the output of the genetic algorithm
fitness value. The error combination of the square root of the angular acceleration of the motor shaft, the
decelerator and differential assembly and the half shaft is adopted as the input of the fuzzy inference controller
and the PID controller, which can significantly simplify the fuzzy rule and the structure of the controllers.

Results:
The proposed method significantly reduces the torque amplitude of the motor shaft, the decelerator
and differential assembly, the transmission half shaft, and the overall vibration amplitude. The maximum
reduced vibration amplitude change is up to 41.22% and 87.04% respectively after parameter optimization
and fuzzy PID control in the example.

Conclusion:
The comprehensive utilization of parameter optimization and adaptive fuzzy PID control
can successfully suppress the torsional vibration phenomenon of the power transmission system,
and the torsional amplitude of the angular velocity and angular acceleration decreases significantly.
Therefore, the vehicle noise can be reduced and the stability can be improved. The study lays a foundation
for solving the torsional vibration problem and the mechanical optimal design of the vehicle
transmission system.
Role of Lacosamide in Preventing Pentylenetetrazole Kindling-Induced Alterations in the Expression of the Gamma-2 Subunit of the GABAA Receptor in Rats
Authors: Gáll Zsolt , Kelemen Krisztina , Mihály István , Salamon Pál , Miklóssy Ildikó , Zsigmond Brigitta , Kolcsár Melinda 
Published: January 2020

Background:
Epilepsy remains challenging to treat still no etiologic treatment has been identified,
however, some antiepileptic drugs (AEDs) are able to modify the pathogenesis of the disease.
Lacosamide (LCM) has been shown to possess complex anticonvulsant and neuroprotective actions,
being an enhancer of the slow inactivation of voltage-gated sodium channels, and it has the potential to
prevent epileptogenesis. Recent evidence has shown that LCM indirectly improves the function of
GABAA receptors. Receptors at most GABAergic synapses involve the gamma-2 subunit, which contributes
to both phasic and tonic inhibition, and its presence assures benzodiazepine sensitivity. Moreover,
mutant gamma-2 subunits were associated with generalized epilepsy syndromes. In animal models,
the expression of the gamma-2 subunit of the gamma-aminobutyric acid A receptor (GABAAg2) was
shown to be increased in pentylenetetrazole (PTZ)-induced chemical kindling in Wistar rats.

Objective:
This study hypothesized that LCM might affect the kindling process by influencing the
expression of GABAA receptors in the hippocampus.

Methods:
The gene and protein expression levels of the GABAAg2 were studied using RT-qPCR and
immunofluorescent staining.

Results:
It was found that LCM treatment (10 mg/kg i.p. daily for 57 days) reduced the maximal
intensity of the PTZ-induced seizures but did not prevent kindling. On the other hand, LCM treatment
reverted the increase of mRNA expression of GABAAg2 in the hippocampus and prevented the
decrease of GABAAg2 protein in the hippocampal CA1 region.

Conclusion:
LCM could exhibit modulatory effects on the GABAergic system of the hippocampus that
may be independent of the anticonvulsant action.
A QoS Metric Approach for Web Service Pertinence for the Cloud
Authors: Singh Jayraj , Dhasarathan Chandramohan 
Published: January 2020

Background:
Cloud computing is a very emerging technology today and playing an important role to provide the services through internet. Users are getting enormous benefits like high accessability of available application resources, faster time to market, fast development, deployment, lower start-up, operations cost and much more. Cloud offers us to utilize the benefits through web services.

Objective:
The degrading factors such as low Quality of Service, security factors, trustless and less resource management in cloud leads to service suitability issue. In service oriented computing finding an efficient QoS based web service on the web is very challenging task.

Methods:
A quality of service metric approach is proposed to identify the suitability of web services
and ensuring the deployability in the cloud era. The suitability of web service is examined for
its suitability cases (simple suitable, average suitable and best suitable) with respect to the quality of
service attribute.

Results:
The experimental setup, first the suitability scores calculated with the essential non-functional quality of service attribute information is identified from the set of services. However, the verification process expressed using the state transition diagram.

Conclusion:
The proposed system is simulated using JFLAP-a java package tool and the result shows the service suitability for the cloud. In this context the efficiency of identifying service suitability for deploying in the cloud gets verified using automated theory without affecting the performance of the cloud service.
POTENTIAL HOLISTIC PREVENTIVE AND THERAPEUTIC EFFECTS OF GARCINIA MANGOSTANA EXTRACT OR ISOLATES IN TYPE 2 DIABETES MELLITUS: A REVIEW
Authors: HANRAHAN JOANNA ERIN , SUBROTO PRISCILLA AYA MAHESWARI , PRIAWAN RAHARDI PRASETIA , KRISNAMURTI DESAK GEDE BUDI 
Published: January 2020

Objective: The need for long-term medication in diabetes mellitus has led to a search for herbal medicines as alternative treatments. Several studies have shown that extract or isolates of Garcinia mangostana can help prevent and treat type 2 diabetes mellitus (T2DM).
Methods: This review was conducted by searching various databases, including PubMed, ClinicalKey, ScienceDirect, and EBSCOhost. We analyzed papers published within the previous 10 y.
Results: All in vitro, in vivo, and clinical studies that evaluated the pharmacological effects of extract or isolates of G. mangostana in T2DM were reviewed. G. mangostana was found to suppress adipogenesis and regulate lipid homeostasis, thus improving lipid profiles and preventing T2DM. G. mangostana also demonstrated hypoglycemic properties, including the ability to decrease fasting blood glucose and mildly increase pancreatic β-cell numbers and activity. The mangosteen-treated group in one study showed a decrease in Homeostatic Model Assesment for Insulin Resistance (HOMA-IR), indicating improved insulin sensitivity, along with a significant decrease in the high-sensitivity CRP (hs-CRP) levels. Histopathology showed that the α-mangostin-treated group had less damage to pancreatic β cells, healthier hepatocytes and central veins, and less glomerular and tubular epithelial necrosis than the diabetic control group. Moreover, the antioxidant effect of G. mangostana was shown to protect against the micro-and macrovascular damage caused by T2DM.
Conclusion: Extract or isolates of G. mangostana possess strong potential to prevent and treat T2DM. Further research evaluating long-term outcome biomarkers in humans is needed to confirm the drug’s glycemic control capacity.
EFFECT OF LUNASIN-RICH SOYBEAN EXTRACT UPON TNF-α EXPRESSION ON COLONIC EPITHELIAL CELLS OF MICE INDUCED BY AZOXYMETHANE/DEXTRAN SODIUM SULFATE
Authors: KUSMARDI KUSMARDI , TAMARA RENATA , ESTUNINGTYAS ARI , TEDJO ARYO 
Published: January 2020

Objective: Colorectal cancer (CRC) contributes to 9.7% of all cancer, and its pathogenesis is related to chronic inflammation. However, current cancer therapy options are lacking, and peptide in food has become popular among researchers because it is cheap, easy to get, has a low toxicity, and is a promising cancer-preventing agent. This research aimed to investigate whether lunasin from soybeans can reduce the expression of pro-inflammatory cytokine TNF-α in colonic epithelial cells.
Methods: Thirty Swiss Webster mice were randomly allocated to six groups. One group was normal, and in five groups, carcinogenesis was induced using azoxymethane (AOM) and dextran sodium sulfate (DSS). The mice were then given nothing (negative control), aspirin (positive control), and lunasin-rich soybean extract (LSE) in three different doses (250, 300, and 350 mg/kgBW) for four weeks. Distal colon tissue was immunohistochemically stained and then observed under a light microscope with 400X magnification to count epithelial cells, based on their color. The index was calculated using optical density scores.
Results: LSE was shown to decrease the expression of tumor necrosis factor (TNF)-α. This decrease was statistically significant between the negative control and a dose of 300 mg/kgBW (p=0.016) and 350 mg/kgBW (p=0.009), yet it was not significant with a dose of 250 mg/kgBW (p=0.754).
Conclusion: a dose of 300 mg/kgBW or higher of LSE can reduce the expression of TNF-α.
ANTIOXIDATIVE ACTIVITY AND PHYTOCHEMISTRY PROFILE OF HIBISCUS SABDARIFFA HERB EXTRACTS
Authors: FITROTUNNISA QOTRUNNADA , ARSIANTI ADE , TEJAPUTRI NADZILA ANINDYA , QORINA FONA 
Published: January 2020

Objective: Hibiscus sabdariffa, known as Roselle, is a widely-cultivated herb in Indonesia and has been consumed as an herbal drink due to its medicinal properties. The purpose of this research is to identify the antioxidant activity and phytochemical profile of Hibiscus sabdariffa.
Methods: The Hibiscus sabdariffa samples were extracted and macerated with three different organic solvents: ethyl acetate, ethanol, and n-hexane. These extracts were then analyzed using thin layer chromatography (TLC) and phytochemical tests to identify the extracts’ secondary metabolites. The extracts’ antioxidant activity was evaluated using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method.
Results: The phytochemistry tests were positive for glycosides, alkaloids, steroids, triterpenoids, tannins, and flavonoids. The TLC analysis revealed that the extracts containing two to three organic compounds. The ethanol Hibiscus sabdariffa extracts with an IC50 value 103.63 ppm showed stronger antioxidant activity than the ethyl acetate extract.
Conclusion: Ethanol Hibiscus sabdariffa extracts may be a potential source of natural antioxidant.
EFFECT OF LUNASIN-ENRICHED SOY EXTRACT ON HISTONE DEACETYLASE EXPRESSION IN DISTAL COLON EPITHELIAL CELLS FROM AOM/DSS-INDUCED MICE
Authors: KUSMARD KUSMARDI , TARIGAN TIFFANY ROSA SUDARSO , ESTUNINGTYAS ARI , TEDJO ARYO 
Published: January 2020

Objective: Lunasin peptide, with its chemopreventive and chemotherapeutic abilities, is known to affect carcinogenesis via epigenetic regulation involving histone acetylation. This study investigated lunasin, which can be found in soy, and its effects towards histone deacetylase (HDAC] expression in a mouse model of carcinogenesis.
Methods: Thirty Swiss Webster mice were grouped into normal, positive control, negative control, and experimental groups. Except for the normal group, mice underwent carcinogenesis induction through azoxymethane (AOM] and dextran sodium sulfate (DSS] injection. Experimental mice received lunasin-enriched soy extract at a dosage of 250 mg/kg BW (kilogram body weight), 300 mg/kg BW, and 350 mg/kg BW for 4 w. Distal colon samples were stained by using immunohistochemistry (IHC]. HDAC expression was measured by IHC optical density score.
Results: Average HDAC expression was 202.4% in the normal group, 239.3% in the negative control, 175.25% in the positive control, 202.03% at 250 mg/kg BW dose, 219.53% at 300 mg/kg, and 166.68% at 350 mg/kg BW. There was no significant difference between HDAC expression at 250 mg/kg BW and 300 mg/kg BW soy extract. However, at 350 mg/kg BW soy extract there were significant changes in HDAC expression.
Conclusion: lunasin in soy extract at a 350 mg/kg BW dose can decrease HDAC expression in a colorectal cancer carcinogenesis model.
EFFICACY OF SILODOSIN VS TAMSULOSIN IN PATIENTS WITH BENIGN PROSTATIC HYPERPLASIA: A DOUBLE-BLIND RANDOMIZED CLINICAL TRIAL
Authors: ARIWICAKSONO S. T. CAHYO , PRASETYO ROBERTUS B , PRASADJA NINDRA , UTOMO NUGROHO B. 
Published: January 2020

Objective: Benign prostatic hyperplasia (BPH) is a nonmalignant enlargement of the prostate associated with aging. BPH can cause lower urinary tract syndrome (LUTS). Medical therapy for patients with moderate and severe LUTS symptoms comprises a-1 adrenergic receptor antagonists. This study aimed to determine whether there are differences in the international prostate symptom score (IPSS) and maximal flow rate (Qmax) of patients with BPH receiving either silodosin or tamsulosin over 12 w.
Methods: This study was a double-blind randomized clinical trial. Subjects were 50 men aged ³ 50 y diagnosed with BPH with an IPSS ³ 8 at the Gatot Soebroto Indonesian Army Hospital. The participants received either silodosin or tamsulosin. Their IPSS and Qmax were assessed at the initial assessment and after 4, 8, and 12 w of treatment.
Results: The initial median IPSS was 15 in the tamsulosin group and 17 in the silodosin group (P = 0.808). After 12 w of therapy, the median IPSS decreased to 9 in the tamsulosin group and 10 in the silodosin group (P = 0.186). The initial median Qmax was 10.1 ml/s in the tamsulosin group and 10.9 ml/s in the silodosin group (P = 0.290). After 12 w of therapy, the median Qmax increased to 12.1 ml/s in the tamsulosin group and 11.9 ml/s in the silodosin group (P = 0.969). Although the differences between groups were not significant, the initial and 12-week IPSS and Qmax values differed significantly within each group.
Conclusion: There were no significant between-group differences in the IPSS or Qmax after 12 w of therapy. However, both silodosin and tamsulosin produced significant differences between initial and 12-week assessments of IPSS and Qmax.
EFFECTIVENESS OF WET COMBING COMPARED WITH 1% PERMETHRIN LOTION FOR THE TREATMENT OF PEDICULOSIS CAPITIS
Authors: SUNGKAR SALEHA , DWINASTITI YASMINE A. , HASWINZKY ROSE A. , IRMAWATI FANNY P. , WARDHANA AJI W. , SUDARMONO PRATIWI , BUNTARAN STANLEY 
Published: January 2020

Objective: Pediculus humanus capitis is an ectoparasite that sucks blood from the patient’s scalp. Gamma-hexachlorocyclohexane is an effective treatment but its use has been discontinued because of its neurotoxicity. Although the replacement pediculicide is permethrin, this is not affordable for everyone, and thus the recommended treatment is wet combing. The aim of this study is to evaluate the effectiveness of permethrin compared with wet combing for eliminating pediculosis.
Methods: This study was a randomized controlled trial (RCT) conducted in a boarding school in Bogor District; the data were collected in July–August, 2018. Subjects were regarded as infested if lice were found during examination. Infested subjects were divided into two groups. In the permethrin group, the hair was wetted with permethrin lotion and left for 10 min. Afterwards, a fine-toothed comb was used to remove lice, and then the subjects were instructed to wash their hair with shampoo. On day 14, each subject’s head was reexamined to determine the cure rate. The wet-combing group was treated similarly but using conditioner instead of permethrin.
Results: Of 121 subjects, 88.4% were infested with head lice. The cure rate after one week of treatment was 66% in the permethrin group and 63% in the wet-combing group. After a two-week course of treatment, the cure rate increased to 94% in permethrin group and 89% in the wet-combing group. There was no significant difference in cure rate between permethrin and wet combing after a one-(P = 0.740) or two-week (P = 0.507) course of treatment.
Conclusion: The prevalence of pediculosis capitis in a boarding school in Bogor District was 88.4%. Wet combing treatment was as effective as permethrin for treating pediculosis capitis.
PHALERIA MACROCARPA LEAF EXTRACT-CHITOSAN NANOPARTICLES SUPRESS ANGIOGENESIS INDUCED BY DEXTRAN SODIUM SULFATE IN MICE COLON
Authors: KUSMARDI KUSMARDI , TUMPU NUR AFIAHUDDIN , ESTUNINGTYAS ARI 
Published: January 2020

Objective: Although an anti-inflammatory effect of Phaleria macrocarpa (Mahkota Dewa in Indonesian) leaf extract has been reported, the extract shows toxicity as the dose increases. Chitosan nanoparticles are known to have transport properties that can enhance the targeting of active compounds to tissues at a lower dose. The present study sought to determine whether the extract in chitosan nanoparticles can suppress angiogenesis in the colon tissue of mice.
Methods: We determined the antiangiogenic effect in 6 groups Swiss Webster mice: normal (N) group, negative control (NC) administered drinking water containing DSS 2% w/v (7 d) and followed by water without DSS (7 d) in 3 cycles, 12.5 and 25 mg leaf extract of P. macrocarpa/mouse (EPM 12.5 and EPM 25) groups, and 6.25 and 12.5 mg leaf extract of P. macrocarpa in chitosan nanoparticles/mouse (NPPM 6.25 and NPPM 12.5) groups. Hematoxylin and eosin-stained samples was performed to determine the amount of angiogenesis in the colon tissue.
Results: The angiogenesis in the NPPM 12.5 (p = 0.105) and EPM 25 (p = 0.07) groups was not significantly different to that in the negative control group (administered DSS alone). By contrast, angiogenesis in the EPM 12.5 (p = 0.03) and NPPM 6.25 (p = 0.02) groups was significantly less than that in the DSS group.
Conclusion: Angiogenesis in the colon tissue of mice was reduced by the extract with or without chitosan nanoparticles. The greatest reduction was found for the 12.5 mg/mouse dose of P. macrocarpa leaf extract in chitosan nanoparticles.
EVALUATION OF [11C]MPC-6827 AS A MICROTUBULE TARGETING PET RADIOTRACER IN CANCER CELL LINES
Authors: KUMAR J. S. DILEEP , PRABHAKARAN JAYA , DAMUKA NARESH , HINES JUSTIN W. , KRIDEL STEVEN J. , MANN J. JOHN , MINTZ AKIVA , SOLINGAPURAM SAI KIRAN KUMAR 
Published: January 2020

Objective: The objective of this study was to evaluate the uptake and specificity of [11C]MPC-6827, a MT targeted PET ligand in prostate, glioblastoma and breast cancer cells.
Methods: [11C]MPC-6827 was synthesized by reacting corresponding desmethyl precursors with [11C]CH3I in a GE-FX2MeI/FX2M radiochemistry module. In vitro binding of [11C]MPC-6827 was performed in breast cancer MDA-MB-231, glioblastoma (GBM) patient-derived tumor (GBM-PDX), GBM U251 and prostate cancer 3 (PC3) cell lines at 37 °C in quadruplicate at 5, 15, 30, 60, and 90 minute incubation time. The nonspecific bindings were determined by incubation with unlabeled microtubule targeting agents MPC-6827, HD-800, colchicine, paclitaxel and docetaxel (5.0 mM).
Results: [11C]MPC-6827 provided the highest binding in the breast cancer cell, MDA-MB-231, among all the cells studied, with 90% specific binding. [11C]MPC-6827 binds to glioblastoma PDX and U251 cells with ~50% and 40% specific binding, whereas, prostate cancer cell line, PC3 cells showed 40% specific binding. [11C]MPC-6827 also exhibits binding to the taxane and colchicine binding sites of MTs, in MDA-MB-231 cells.
Conclusion: These data indicate that [11C]MPC-6827 can be a promising PET radiotracer for preclinical imaging of the brain and peripheral cancers.