bluuebunny/crossref_metadata_2025_split huggingface dataset:
miR‐511 inhibits proliferation and metastasis of breast cancer cells by targeting FGF4
Authors: Zhang Chao , Yang Ting , Jiang Hongchuan 
Published: February 2020

AbstractBackgroundThe present study aimed to explore the functions and molecular mechanisms of miR‐511 in breast cancer.MethodsA quantitative real‐time polymerase chain reaction (qRT‐PCR) was used to detect miR‐511 levels in breast cancer tissues; a chi‐squared test was used to analyze the relationship between miR‐511 expression level and pathological parameters of breast cancer patients; the proliferation of breast cancer cell lines MDA‐MB‐231 and MCF‐7 was determined by the cell counting kit‐8 (CCK‐8) assay; migration was determined by scratch wound healing assay and transwell assay; TargetScan was used to predict the binding site between the 3'‐untranslated region (3'‐UTR) of fibroblast growth factor 4 (FGF4) and miR‐511; and qRT‐PCR, western blot and a luciferase reporter gene assay were conducted to further validate the targeting relationship between miR‐511 and FGF4.ResultsThe expression level of miR‐511 was lower in breast cancer tissues than that in adjacent normal tissues. Low expression of miR‐511 was associated with larger tumor size, lymph node metastasis and short survival time. In vitro experiments showed that miR‐511 modulated the proliferation and metastasis of breast cancer cells. It was also confirmed that miR‐511 directly targeted 3'‐UTR of FGF4 and reduced its expression, and FGF4 overexpression reversed the effect of miR‐511 on the malignant phenotypes of breast cancer cells.ConclusionsThe results obtained in the present study demonstrate that miR‐511 inhibits breast cancer proliferation and metastasis by down‐regulating FGF4 expression, which may be helpful in the development of new treatment strategies for breast cancer.
Bacterial cell leakage potential of newly synthesized quinazoline derivatives of 1,5‐benzodiazepines analogue
Authors: Misra Apoorva , Dwivedi Jaya , Shukla Shruti , Kishore Dharma , Sharma Swapnil 
Published: February 2020

AbstractNovel quinazoline embellished analogues of 1,5‐benzodiazepine (6, 9, 13, 17, 21 and 24) were synthesized using one‐pot domino approach. Structure of the compounds synthesized, were established with the help of IR, 1H NMR, 13C NMR and mass spectral data. Further, antibacterial activity, FE‐SEM imaging and cell leakage study was also performed.
TEG011 persistence averts extramedullary tumor growth without exerting off-target toxicity against healthy tissues in a humanized HLA-A*24:02 transgenic mice
Authors: Johanna Inez , Hernández-López Patricia , Heijhuurs Sabine , Bongiovanni Laura , de Bruin Alain , Beringer Dennis , van Dooremalen Sanne , Shultz Leonard D. , Ishikawa Fumihiko , Sebestyen Zsolt , Straetemans Trudy , Kuball Jürgen 
Published: February 2020

Abstract
γδT cells play an important role in cancer immunosurveillance and are able to distinguish malignant cells from their healthy counterparts via their γδTCR. This characteristic makes γδT cells an attractive candidate for therapeutic application in cancer immunotherapy. Previously, we have identified a novel CD8α-dependent tumor-specific allo-HLA-A*24:02-restricted Vγ5Vδ1TCR with potential therapeutic value when used to engineer αβT cells from HLA-A*24:02 harboring individuals. αβT cells engineered to express this defined Vγ5Vδ1TCR (TEG011) have been suggested to recognize spatial changes in HLA-A*24:02 present selectively on tumor cells but not their healthy counterparts. However, in vivo efficacy and toxicity studies of TEG011 are still limited. Therefore, we extend the efficacy and toxicity studies as well as the dynamics of TEG011 in vivo in a humanized HLA-A*24:02 transgenic NSG (NSG-A24:02) mouse model to allow the preparation of a first-in-men clinical safety package for adoptive transfer of TEG011. Mice treated with TEG011 did not exhibit any graft-versus-host disease-like symptoms and extensive analysis of pathologic changes in NSG-A24:02 mice did not show any off-target toxicity of TEG011. However, loss of persistence of TEG011 in tumor-bearing mice was associated with the outgrowth of extramedullary tumor masses as also observed for mock-treated mice. In conclusion, TEG011 is well tolerated without harming HLA-A*24:02+ expressing healthy tissues, and TEG011 persistence seems to be crucial for long-term tumor control in vivo.
The first two cases of 2019‐nCoV in Italy: Where they come from?
Authors: Giovanetti Marta , Benvenuto Domenico , Angeletti Silvia , Ciccozzi Massimo 
Published: February 2020

AbstractA novel Coronavirus, 2019‐nCoV, has been identified as the causal pathogen of an ongoing epidemic, with the first cases reported in Wuhan, China, last December 2019, and has since spread to other countries worldwide, included Europe and very recently Italy. In this short report, phylogenetic reconstruction was used to better understand the transmission dynamics of the virus from its first introduction in China focusing on the more recent evidence of infection in a couple of Chinese tourists arrived in Italy on 23rd January 2020 and labeled as Coronavirus Italian cases. A maximum clade credibility tree has been built using a dataset of 54 genome sequences of 2019‐nCoV plus two closely related bat strains (SARS‐like CoV) available in GenBank. Bayesian time‐scaled phylogenetic analysis was implemented in BEAST 1.10.4. The Bayesian phylogenetic reconstruction showed that 2019‐2020 nCoV firstly introduced in Wuhan on 25 November 2019, started epidemic transmission reaching many countries worldwide, including Europe and Italy where the two strains isolated dated back 19 January 2020, the same that the Chinese tourists arrived in Italy. Strains isolated outside China were intermixed with strains isolated in China as evidence of likely imported cases in Rome, Italy, and Europe, as well. In conclusion, this report suggests that further spread of 2019‐nCoV epidemic was supported by human mobility and that quarantine of suspected or diagnosed cases is useful to prevent further transmission. Viral genome phylogenetic analysis represents a useful tool for the evaluation of transmission dynamics and preventive action.
Full‐mouth disinfection effects on gingival fluid calprotectin, osteocalcin, and N‐telopeptide of Type I collagen in severe periodontitis
Authors: Afacan Beral , Çınarcık Serhat , Gürkan Ali , Özdemir Güven , İlhan Harika Atmaca , Vural Caner , Köse Timur , Emingil Gülnur 
Published: February 2020

AbstractBackgroundTo compare the effects of full‐mouth disinfection (FMD) and full‐mouth ultrasonic debridement (FMUD) on clinical, microbiological and biochemical parameters with conventional quadrant‐wise scaling and root planning (Q‐SRP) in severe chronic periodontitis.MethodsIn the present prospective randomized controlled clinical trial with three parallel arms (#NCT04038801), 60 chronic periodontitis patients were randomly assigned to three study groups by a consecutive number in ascending order: FMD (n = 20), FMUD (n = 20), and Q‐SRP (n = 20). All measurements and treatments were performed by the same investigator. At baseline, gingival crevicular fluid (GCF) and subgingival plaque were collected and clinical periodontal parameters were recorded. Ultrasonic debridement was completed within 24 hours in FMD and FMUD groups. Chlorhexidine gluconate was used for FMD. Q‐SRP was performed by hand instruments per quadrant at 1‐week‐intervals. Clinical measurements and sampling were repeated at 1, 3, and 6 months after treatment. Real‐time PCR was used for quantitative analysis of Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythia, Fusobacterium nucleatum, and total bacteria count. GCF Calprotectin, osteocalcin, and N‐telopeptide of type I collagen (NTx) levels were analyzed by ELISA. The changes of GCF biomarker levels after treatment between groups were the primary outcomes.ResultsNo harm was observed. All treatment strategies resulted in significant improvements in all clinical parameters (P < 0.05), with no significant differences between study groups at all time‐points (P ˃ 0.05). Aggregatibacter actinomycetemcomitans was significantly decreased in FMD compared to FMUD and Q‐SRP at 6 months (P < 0.05). Although GCF NTx total amounts increased in all groups during the study period, this increase was less prominent in full‐mouth groups at three time points after treatment (P < 0.05).ConclusionsPresent results represent the short‐term effects. Full‐mouth treatment approaches offered limited beneficial effects on microbiological and biochemical parameters over quadrant‐wise approach. All three treatment strategies can be recommended in the management of severe chronic periodontitis.
Investigation of the Stokes to anti‐Stokes ratio for germanium nanowires obtained by electrochemical deposition
Authors: Pavlikov Alexander V. , Forsh Pavel A. , Kashkarov Pavel K. , Gavrilov Sergey A. , Dronov Alexey A. , Gavrilin Ilya M. , Volkov Roman L. , Borgardt Nikolay I. , Bokova‐Sirosh Sofia N. , Obraztsova Elena D. 
Published: February 2020

AbstractGermanium nanowires fabricated by the electrochemical liquid–liquid–solid deposition from water solutions on titanium coated silicon substrate were studied by Raman spectroscopy in order to estimate the heating effect of exciting radiation. The Stokes to anti‐Stokes ratio was determined for crystalline Ge wafer and for nanowires with different mean diameters at two different excitation wavelengths. It was demonstrated that local heating by exciting laser radiation can lead to an increase in temperature sufficiently high for crystallization of initially amorphous Ge nanowires. The results presented may be of great use for fabricating devices, such as metal ion batteries or thermoelectric elements, based on Ge nanowire.
Effects of Tetragenococcus halophilus and Candida versatilis on the production of aroma‐active and umami‐taste compounds during soy sauce fermentation
Authors: Zhang Lijie , Zhang Ling , Xu Yan 
Published: February 2020

AbstractBACKGROUNDSoy sauce is a well‐known condiment worldwide. However, the high salt content in soy sauce contributes to the high intake of sodium salt, which usually results in high blood pressure. High salt soy sauce usually has the better quality (aroma and taste) than low salt. Tetragenococcus halophilus and Candida versatilis are important starters for soy sauce fermentation. It is of urgent need to explore what the effect of these two strains on the aroma‐ and taste‐attributes of soy sauce to achieve high quality fermentation with low salt.RESULTSIn this study, aroma‐active and taste compounds in soy sauce were reviewed and listed. Then, soy sauce fermentation inoculated with different combinations of T. halophilus (at different inoculated time) and C. versatilis were completed. Aroma‐active and taste compounds in different samples were quantified. Multivariate analysis was used to analyze these data. The aroma‐active compounds which were highly related to the inoculation of T. halophilus and C. versatilis were found. Meanwhile, the addition time of T. halophilus could also be highly related to the production of aroma‐active compounds. More importantly, T. halophilus was highly correlated with the production of umami‐taste compounds in soy sauce, including aspartic acid, glutamic acid, alanine and N
‐succinyl‐glutamic acid.CONCLUSIONThese results will provide a better understanding of the effects of T. halophilus and C. versatilis on the formation of significant aroma‐active and umami‐taste constituents in soy sauce. Furthermore, it will be helpful to realize fermentative control of soy sauce with high quality at low salt. © 2020 Society of Chemical Industry
Changes in microbial, physiochemical, and functional properties of pasteurized liquid whole egg during refrigerated storage
Authors: Wang Jing , Gu Luping , Su Yujie , Chang Cuihua , Xu Lilan , Yang Yanjun , Li Junhua 
Published: February 2020

AbstractBACKGROUNDLiquid eggs have the advantages of high hygiene security, easy use, and convenient transportation, but their shelf life is only limited to 2~3 weeks. The microbial, physiochemical, and functional properties of pasteurized LWE were investigated in this study to evaluate the quality of pasteurized liquid whole egg (LWE) during refrigerated storage.RESULTSThe tested shelf life of the pasteurized LWE was 16 days when stored at 4 °C. During refrigerated storage, Pseudomonas gradually became the dominant bacterium in LWE following lactic acid bacteria, although the initial number of Pseudomonas after pasteurization was relatively limited (< 10 CFU mL−1). A total of 23 strains, including six Pseudomonas strains, were obtained. The pH of pasteurized LWE decreased with the growth of microorganisms, while the content of total volatile basic nitrogen (TVB‐N) increased curvilinearly. The average particle size increased almost continuously until the sample reached its shelf life. The functional properties of pasteurized LWE were also reduced after a week of refrigerated storage at 4 °C when the microorganisms in pasteurized LWE entered an exponential growth period and the TVB‐N content of pasteurized LWE reached its first peak.CONCLUSIONDuring refrigerated storage, Pseudomonas was the dominant bacterium in LWE next to lactic acid bacteria. After a week of refrigerated storage at 4 °C, the particle size of LWE increased, while the functional properties of LWE reduced. This study provides a basis for extending the shelf life of liquid egg products in future research. © 2020 Society of Chemical Industry
Molecular action mechanism of anti‐inflammatory hydrolysates obtained from brewers' spent grain
Authors: Cian Raúl E , Hernández‐Chirlaque Cristina , Gámez‐Belmonte Reyes , Drago Silvina R , Sánchez de Medina Fermín , Martínez‐Augustin Olga 
Published: February 2020

AbstractBACKGROUNDBrewers’ spent grain (BSG) is a relevant, protein‐rich by‐product of the brewing process. Protein hydrolysates from different sources exert immune‐regulatory actions activating toll‐like receptors (TLRs), nuclear factor kappa B (NFκB), and mitogen‐activated protein kinases (MAPKs). Effects of gastrointestinal digestion have been poorly studied. Here, we studied the immune‐regulatory effect of BSG hydrolysates, and their in‐vitro‐digested products, on rat splenocytes, macrophages, and T lymphocytesRESULTSIn primary cultures of rat spleen cells, BSG hydrolysates induced interleukin 10 and tumor necrosis factor production in basal conditions. Under stimulation with lipopolysaccharide or concanavalin A, hydrolysates further induced interleukin 10 production. Tumor necrosis factor and interferon‐γ were inhibited in lipopolysaccharide‑ and concanavalin‐A‐stimulated cells respectively. In vitro gastrointestinal digestion attenuated the observed effects. Splenic macrophages and T lymphocytes behaved in a similar fashion. In spleen cells from TLR2−/− and TLR4−/− mice, immune‐regulatory effects were greatly reduced or abrogated. The study of signal transduction pathways indicated a major involvement of NFκB, and the contribution of MAPKs p38, c‐Jun N‐terminal kinase, and extracellular signal‐regulated kinases 1 and 2.CONCLUSIONBSG hydrolysates, like those obtained from other food sources, regulate the immune response, involving TLR2 and TLR4 and the activation of NFκB and MAPKs, an effect partly maintained after in vitro gastrointestinal digestion. Our data support the hypothesis of a shared, rather unspecific, mechanism of action of protein hydrolysates. © 2020 Society of Chemical Industry
Complications after thigh sarcoma resection
Authors: Elswick Sarah M. , Curiel Daniel A. , Wu Peter , Akhavan Arya , Molinar Vanessa E. , Mohan Anita T. , Sim Frank H. , Martinez‐Jorge Jorys , Saint‐Cyr Michel 
Published: February 2020

AbstractBackground and ObjectivesStandard treatment for extremity sarcoma is limb‐sparing surgery often with radiation, but complications occur frequently. We sought to determine factors predictive of wound complications after thigh sarcoma resection and reconstruction while analyzing trends over time.MethodsWe reviewed all thigh defects requiring plastic surgeon reconstruction following sarcoma resection at our institution from 1997 to 2014. Patient demographics, comorbidities, operative characteristics, multi‐modality therapies, and complications were analyzed. Wound complications were: infection, dehiscence, seroma, hematoma, or partial/total flap loss.ResultsThere were 159 thigh reconstructions followed for 30 months on average. Eighty‐seven percent of patients underwent radiation and 42% had chemotherapy. Almost half (49.1%) had a complication. The most common wound complication was surgical site infection (23.3%) followed by dehiscence (19.5%), and seroma (10.7%). Less common were partial (2.5%) or total flap loss (0.6%). Reoperation was required in 21 patients (13.2%). Tobacco use, older patient age, cardiac disease, and higher body mass index were independently associated with wound complications. Complications trended towards decreasing over time, but this was not statistically significant.ConclusionsTobacco use, cardiac disease, and higher body mass index, but not the timing of reconstruction, appear to increase the risk of wound complications after thigh soft tissue sarcomas resection and plastic surgery reconstruction.
When does nutrient management sequester more carbon in soils and produce high and stable grain yields in China?
Authors: Waqas Muhammad Ahmed , Li Yu'e , Lal Rattan , Wang Xiaohan , Shi Shengwei , Zhu Yongchang , Li Jianling , Xu Minggang , Wan Yunfan , Qin Xiaobo , Gao Qingzhu , Liu Shuo 
Published: February 2020

AbstractIn agro‐ecosystems, fertilization practices must accomplish high and stable crop productivity, with maximum soil organic carbon (SOC) sequestration to address climate change and food security challenges. However, the impacts of these practices on SOC and crop yields are variable over the long‐term duration, and an improved understanding of the factors influencing SOC sequestration and sustainable productivity is still needed to provide evidence‐based management decisions. Therefore, we conducted a meta‐analysis to evaluate the impact of long‐term (≥10 years) application of different fertilizer management practices adapted across China on crop productivity, yield sustainability, and SOC sequestration. Results indicated that unbalanced mineral fertilizer (UMF), balanced mineral fertilizer (BMF), organic fertilizers (OF), combined unbalanced mineral and OF, and combined balanced mineral and organic fertilizers (BMOF) significantly enhanced the grain yield, and SOC sequestration compared with control (p < .05). For UMF, the increases in SOC and grain yields were least among fertilization practices. Comparing OF, BMF mostly produced more grain yields, but with a slight increase in C sequestration. Highest SOC sequestration rate of 0.43 Mg C ha−1 yr−1 was recorded in BMOF, among all the treatments. The data obtained indicated that SOC sequestration is highly time‐dependent. Irrespective of fertilization mode, SOC gradually increased and attained the peak of sequestration rate in the initial two decades rather than at later stages of fertilizer addition. The linear fitted model indicated that an increase in SOC sequestration benefits sustainable productivity. The available data indicate that crop yields can be improved by 143 kg ha−1 for rice (Oryza sativa), 255 kg ha−1 for maize (Zea mays), and 202 kg ha−1 for wheat (Triticum aestivum) with every 1 Mg ha−1 increase in SOC stock by fertilization in the root zone. In crux, BMOF can maintain and improve soil quality while producing high and stable crop yields.
Organic sulfur: A spatially variable and understudied component of marine organic matter
Authors: Longnecker Krista , Oswald Lisa , Kido Soule Melissa C. , Cutter Gregory A. , Kujawinski Elizabeth B. 
Published: February 2020

AbstractSulfur (S) is a major heteroatom in organic matter. This project evaluated spatial variability in the concentration and molecular‐level composition of organic sulfur along gradients of depth and latitude. We measured the concentration of total organic sulfur (TOS) directly from whole seawater. Our data reveal high variability in organic sulfur, relative to established variability in total organic carbon or nitrogen. The deep ocean contained significant amounts of organic sulfur, and the concentration of TOS in North Atlantic Deep Water (NADW) decreased with increasing age while total organic carbon remained stable. Analysis of dissolved organic matter extracts by ultrahigh resolution mass spectrometry revealed that 6% of elemental formulas contained sulfur. The sulfur‐containing compounds were structurally diverse, and showed higher numbers of sulfur‐containing elemental formulas as NADW moved southward. These measurements of organic sulfur in seawater provide the foundation needed to define the factors controlling organic sulfur in the global ocean.
All at once or one at a time? The effect of simultaneous versus sequential discount presentation on store patronage intentions
Authors: DelVecchio Devon , Wang Jessie J. , Brigden Neil 
Published: February 2020

AbstractRetailers often employ store flyers, be they in print or digital form, to drive store traffic. A fundamental difference in the presentation of multicomponent information, such as the multiple discounts presented in flyers, is whether the components are displayed simultaneously (all at once) of sequentially (one at a time). Yet a little extant research examines how these different presentations affect individuals' responses to retailer price promotions. Three experiments demonstrate that a sequential display of price discounts is associated with more positive store patronage intentions. Evidence, gleaned by both measuring and manipulating the process by which the discounts are evaluated, implicates a greater sense of accumulating benefit with each successive discount when presented sequentially as the driver of the cross‐format difference in patronage intentions.
Poly(ethylene glycol) Diacrylate Based Electro‐Active Ionic Elastomer
Authors: Rajapaksha Chathuranga Prageeth Hemantha , Feng Chenrun , Piedrahita Camilo , Cao Jinwei , Kaphle Vikash , Lüssem Björn , Kyu Thein , Jákli Antal 
Published: February 2020

AbstractPreparation and low voltage induced bending (converse flexoelectricity) of crosslinked poly(ethylene glycol) diacrylate (PEGDA), modified with thiosiloxane (TS) and ionic liquid (1‐hexyl‐3‐methylimidazolium hexafluorophosphate) (IL) are reported. In between 2µm PEDOT:PSS electrodes at 1 V, it provides durable (95% retention under 5000 cycles) and relatively fast (2 s switching time) actuation with the second largest strain observed so far in ionic electro‐active polymers (iEAPs). In between 40 nm gold electrodes under 8 V DC voltage, the film can be completely curled up (270° bending angle) with 6% strain that, to the best of the knowledge, is unpreceded among iEAPs. These results render great potential for the TS/PEGDA/IL based electro‐active actuators for soft robotic applications.