bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Impact of an Audience Response System on a Summative Assessment, a Controlled Field Study
Authors: Schmidt Thorsten , Gazou Anastasia , Riess Angelika , Riess Olaf , Grundmann-Hauser Kathrin , Falb Ruth , Schadeck Malou , Heinrich Tilman , Abeditashi Mahkameh , Schmidt Jana , Mau-Holzmann Ulrike , Schnabel Kai 
Published: March 2020

Abstract
Background: Audience response systems allow to activate the audience and to receive a direct feedback of participants during lectures. Modern systems do not require any proprietary hardware anymore. Students can directly respond on their smartphone. Several studies reported about a high level of satisfaction of students when audience response systems are used, however their impact on learning success is still unclear.Methods: In order to evaluate the impact of an audience response system on the learning success we implemented the audience response system eduVote into a seminar series and performed a controlled crossover study on its impact on assessments. 154 students in nine groups were taught the same content. In five groups, eduVote was integrated for the first topic while four groups were taught this topic without the audience response systems. For a second topic, the groups were switched: Those groups who were taught before using eduVote were now taught without the audience response system and vice versa. We then analyzed the impact of the audience response system on the students’ performance in a summative assessment and specifically focused on questions dealing with the topic, for which the audience response system was used during teaching. We further assessed the students’ perception on the use of eduVote using questionnaires.Results: In our controlled crossover study we could not confirm an impact of the audience response system eduVote on long-term persistence i.e. the students’ performance in the summative assessment. Our evaluation revealed that students assessed the use of eduVote very positively, felt stronger engaged and better motivated to deal with the respective topics and would prefer their integration into additional courses as well. In particular we identified, that “shy students” profit from the use of an audience response system during teaching.Conclusions: Audience response systems motivate and activate students and increase their engagement during classes. However, their impact on long-term persistence and summative assessments may be limited. Audience response systems, however, specifically allow activating students which cannot be reached by the traditional way of asking questions without such an anonymous tool.
Use of profession-role exchange in an interprofessional student team-based community health service-learning experience
Authors: Wang Jun , Guo Jie , Wang Yubin , Yan Dan , Liu Juan , Zhang Yinghong , Hu Xianmin 
Published: March 2020

Abstract
Background: During interprofessional clinical practice, compared to understanding of one’s own professional role and function, it might be more difficult to clarify the roles and contributions of the other health-care team members because of the inter-professional barrier. In order to provide students the opportunity for real experience with other professions in team environments and enhance their perceptions of other professions’ role, this study developed a comprehensive and multi-dimension extracurricular interprofessional education (IPE) model through designing and integrating a profession-role exchange component, that was medical students as pharmacists or nurses, pharmacy students as physicians or nurses, and nursing students as physicians or pharmacists in the interprofessional health-care student team, into the service learning experience in a real community setting.Methods: In this pre/post-intervention study, the effect of integrated profession-role exchange experiences on the students’ attitudes towards interprofessional collaboration and their role clarification was evaluated among 60 student volunteers (20 medical students, 20 pharmacy students and 20 nursing students). All involved students were divided into the profession-role exchange intervention group and the control group. Subjects in the control group did not participate the profession-role exchange experiences, the other IPE procedures were the same for both groups. Three survey instruments for attitudes toward interprofessional clinical collaboration were respectively used to measure the students’ attitudes toward physician-pharmacist, physician-nurse and nurse-pharmacist collaborations. “Roles and responsibilities” subscale of Readiness for Interprofessional Learning Scale was used to evaluate the overall role clarification during IPEResults: Compared to the control IPE activity, the addition of profession-role exchange component resulted in the significant increase in students’ positive attitudes towards interprofessional collaboration, and the enhancement of students’ role awareness.Conclusions: The profession-role exchange might be more effective and better initiate students to the practice of interprofessional collaboration, and could be used as an effective IPE tool for improving the role awareness of health-care students.
LncRNA Testis-specific transcript, Y-linked 15 (TTTY15) promotes proliferation, migration and invasion of colorectal cancer cells via regulating miR-29a-3p/DVL3 axis
Authors: Cao Ming-zheng , Ba Ying , Li Yue-feng 
Published: March 2020

Abstract
Background: Long non-coding RNA TTTY15 is oncogenic in prostate cancer, however its expression and function in colorectal cancer remain largely unknown.Methods: Paired colorectal cancer samples/adjacent tissues were collected, and the expression levels of TTTY15, miR-29a-3p and disheveled segment polarity protein 3 (DVL3) were examined by quantitative real-time polymerase chain reaction (qRT-PCR). TTTY15 shRNAs were transfected into HT-29 and HCT-116 cell lines using lipofectamine reagent; the proliferation and colony formation were detected by CCK-8 assay and plate colony formation assay. qRT-PCR and western blot were used to analyze the changes of miR-29a-3p and DVL3; luciferase reporter gene assay was used to determine the regulatory relationship between miR-29a-3p and TTTY15, miR-29a-3p and DVL3.Results: TTTY15 was significantly up-regulated in cancerous tissues of colorectal cancer samples, positively correlated with the expression of DVL3, while negatively correlated with miR-29a-3p. After TTTY15 shRNAs were transfected into colorectal cancer cells, the proliferation and metastasis of cancer cells were significantly inhibited. TTTY15 shRNAs could reduce the expression of DVL3 on both mRNA and protein levels, and the luciferase activity of TTTY15 sequence was also inhibited by miR-29a-3p. DVL3 was also validated as a target gene of miR-29a-3p.Conclusion: TTTY15 is abnormally upregulated in colorectal cancer tissues, and it can modulate the proliferation and metastasis of colorectal cancer cells. It function as the ceRNA to regulate the expression of DVL3 by sponging miR-29a-3p.
Identification of potential biomarkers and candidate small molecule drugs in glioblastoma
Authors: Lu Wei-cheng , Xie Hui , Yuan Ce , Li Jin-jiang , Li Zhao-yang , Wu An-hua 
Published: March 2020

Abstract
Background and aioms: Glioblastoma (GBM) is a common and aggressive primary brain tumor, and the prognosis for GBM patients remains poor. This study aimed to identify the key genes associated with GBM and to further analyze the prognostic factors and small molecular drugs. Methods: Three microarray datasets (GSE111260, GSE103227, and GSE104267) were selected to identify the differential expressed genes (DEGs) between GBM and normal tissues. Then, prognosis related DEGs were screened by survival analysis, followed by functional enrichment analysis was performed. The protein-protein interaction (PPI) network was constructed to explore the hub genes associated with GBM. The prognostic of these hub genes in GBM patients were analyzed using The Cancer Genome Atlas (TCGA) database, and the protein expression level of hub genes were validated using the Human Protein Atlas (HPA) databases. Subsequently, the small molecule drugs of GBM were predicted by Connectivity Map (CMAP) database. Results: A total of 78 DEGs related to GBM prognosis were identified, and 10 hub genes with high degree were obtained. The mRNA expression and protein expression levels of CETN2, MKI67, ARL13B, and SETDB1 were overexpressed in GBM tissues, while the expression levels of CALN1, ELAVL3, ADCY3, SYN2, SLC12A5, and SOD1 were down-regulated in GBM tissues. Additionally, these genes were significantly associated with the prognosis of GBM. A total of 98 small molecular drugs were predicted, among these, adiphenine and podophyllotoxin were considered as the potential drugs to treat GBM.Conclusions: Our study provided 10 key genes for diagnosis, prognosis, and therapeutic target for GBM. These findings might contribute to a better comprehension of molecular mechanisms of GBM development, and provide new perspective for further GBM research. However, specific regulatory mechanism of these genes needed further elaboration.
An untargeted investigation into the skin surface lipidome in the West Highland white terrier dog, with and without atopic dermatitis
Authors: Orbell Helen Louise , Cave Nick J , Parry Katharina , Griffin Craig E 
Published: March 2020

Abstract
Background – The skin barrier is important in the pathogenesis of atopic dermatitis and stratum corneum lipids have a critical role. Skin surface lipids have been largely overlooked but also contribute to barrier function. An untargeted approach was used to compare the skin surface lipids from atopic and non-atopic West Highland White terrier dogs. The primary hypothesis was that a difference in the lipidome of atopic and non-atopic dogs would be found and the secondary hypothesis was that affected and unaffected skin would differ in lipid profile.Results – Thirty-nine dogs were classified into one of four disease status groups based on strict criteria. Samples for lipid analysis were collected from affected and unaffected skin, and liquid chromatography/mass spectrometry found 421 lipid soluble features. Ten lipids were positively identified. Statistical analysis could not distinguish between non-atopic and atopic dogs. Partial least squares-discriminant analysis revealed a difference in the lipid profiles from affected and non-affected skin irrespective of disease status. Conclusions – An untargeted approach found a large array of unidentified lipids from the skin surface. There was a difference in the lipidome between affected and unaffected skin that was not related to disease status. Investigation into the lipidome of the skin surface in health and disease is an emerging area of research which could have clinical and therapeutic applications.
Nurses’ Perception Of Leadership Styles, Job Satisfaction, And Contextual Performance
Authors: SEYMEN SEFANUR , YILDIRIM Aytolan 
Published: March 2020

Abstract
Background: This study, which is done using 606 nurses working in state hospitals under the Department Of Institutions For In-Patient Treatment Affiliated To Turkish Republic Of Northern Cyprus Ministry Of Health, aims to investigate the effect of manager-employee relationship on job satisfaction and contextual performance of perceived leadership style.Methods: To determine the effect of leadership and job satisfaction on the contextual performance of the research, a screening model was used from descriptive research methods and a questionnaire consisting of four parts was prepared as data collection tool.Results: Results show that age, gender, duration of work and working position were found to affect job satisfaction and contextual performance in general (p <0,05).Conclusions: This study concludes that personal profiles of nurses are essential determinants of how leadership, job satisfaction, and contextual performance is perceived.
in vitro Chemical and physical toxicity of polystyrene microplastics in human-derived cells
Authors: Choi Daheui , Bang Junah , Kim Taeho , Oh Yoogyeong , Hwang Youngdeok , Hong Jinkee 
Published: March 2020

Abstract
Background With the increase of plastics production, a variety of toxicological studies regarding the microplastics have been reported since the microplastics could be ingested by the human body and cause serious diseases. However, the previous studies have been mainly focused on the toxicity of sphere type microbeads, which may be different from that of the randomly-shaped microplastics in real environment. Here, we have conducted the in vitro toxicology for randomly-shaped microplastics following the hypothesis that (1) physical cytotoxicity is affected from nano-/micro-size roughness in polystyrene (PS) microplastics and (2) chemical toxicity is caused by chemical reagents from microplastics.Results To prepare random shape of PS microplastics, we produced microfragments by ball mill grinding, then analyzed them via various toxicity tests in chemical and physical aspects with various kinds of human-derived cells. Ground PS microplastics were sorted in 3 ranges: 5-25 μm, 25-75 μm and 75-200 μm, and treated up to 1 mg/mL to cells based on weekly human intake of microplastics. We have confirmed that the PS microfragments induced 20 times increased acute inflammation for immune cells, production of reactive oxygen species and cell-death for fibroblasts and cancer cells by release of chemical reagents from microplastics. In addition, when the PS microfragments were in direct contact with the fibroblast and red blood cells, they lead to the lactose dehydrogenase release caused by a cell membrane damage and hemolysis by physical stress of microfragments. This phenomenon was amplified as microfragments concentration and roughness increases, we quantitatively analyzed roughness differences between microplastics, demonstrating that there are strong relationship physical damage of cells and roughness of microplastics.Conclusion We found that the PS microfragments have chemical toxicity. Furthermore, the physical toxicity by PS resulted in cellular membrane damage and correlated with statistically quantified-shape roughness. Therefore, we newly suggested the additional physical toxicity of random shape of microplastics. This provides the evidence of environmental and biological risks on random shape of microplastics.
Diesel exhaust particulates induce neutrophilic lung inflammation by modulating endoplasmic reticulum stress-mediated CXCL1/KC expression in alveolar macrophages
Authors: Kim Dong Im , Song Mi-Kyung , Lee Kyuhong 
Published: March 2020

Abstract

Background Exposure to particular matter (PM)2.5, including diesel exhaust particulates (DEP), has adverse effects on the respiratory system. Endoplasmic reticulum (ER) abnormalities contribute to respiratory disease pathogenesis such as lung inflammation. However, there is little published research on the relationship between DEP exposure and ER stress in the respiratory immune system and especially the alveolar macrophages (AM). Here, we examined ER stress and inflammatory responses in a DEP-induced murine lung inflammation model and in DEP-stimulated AM.Results DEP treatment increased relative lung weight and the number of total cells, neutrophils, and lymphocytes in mouse BALF. Histological examinations also showed that DEP exposure induced neutrophilic lung inflammation and increased the number of DEP-pigmented AM. Western blot analysis showed that BiP and CHOP were relatively upregulated in DEP-induced mouse lung tissues. DEP caused cell damage, increased intracellular reactive oxygen species (ROS), and upregulated the genes associated with inflammation (tumor necrosis factor-α, interleukin [IL]-1β, IL-6, interferon-γ, and toll-like receptor 4) and with ER stress (bound immunoglobulin protein [BiP], CCAAT/enhancer binding protein-homologous protein [CHOP], sXBP-1, and activating transcription factor 4) in AM. Furthermore, DEP stimulation upregulated the gene encoding the chemokine CXCL1/KC in AM.Conclusions DEP may contribute to neutrophilic lung inflammation pathogenesis by modulating ER stress-mediated CXCL1/KC expression in alveolar macrophages.
Clinical Outcomes and influence factors of differentiated thyroid carcinoma with negative preablative stimulated thyroglobulin and iodine-positive lymph nodes: A retrospective analysis
Authors: Wang Zenghua , Li Jiao , Wang Guoqiang , Liu Xinfeng , Xin Xiaotong , Zhang Yingying , Han Na , Wang Xufu , Lu Chenghui 
Published: March 2020

Abstract
Background To investigate the clinical outcome of postoperative differentiated thyroid carcinoma(DTC)patients with negative preablative-stimulated thyroglobulin (ps-Tg) and iodine-positive lymph node after the first radioablation. Methods A total of 136 DTC patients with ps-Tg<2ng/ml and iodine-positive lymph nodeswho were initially treated by 131I at our hospital from May 2015 to February 2018 were enrolled. These patients were followed up for 6-36 months, and then were classified into 3 groups according to the clinical outcomes as follows: excellent response (ER) group, indeterminate response (IDR) group, and structural incomplete response (SIR) group.Univariate and multivariate analyses were performed to assess thedifferences of factors (e.g. age, gender, extraglandular infiltration) between 3 groups. Results The N stage (P=0.001), sizes of the displayed lymph nodes (P<0.001), ps-Tg level (P=0.002), and TgAb level (P=0.001) were significantly different among the 3 groups. The gender (P=0.615), age (P=0.332), the primary tumor size (P=0.311), extrathyroidal invasion (P=0.549), T stage (P=0.944), rate of lymph node metastasis (P=0.170), locations of the displayed lymph nodes (P=0.099), and degree of recurrence risk (P=0.783) were not significantly different among the 3 groups.The corresponding sensitivity, specificity, positive predictive value, and negative predictive values were 93.3%, 82.6%, 86.4%, and 91.3% respectively when the cut-off value of the lymph node size was 7.25 mm. Conclusion For the DTC patients with negative ps-Tg and iodine-positive lymph nodes, postoperative N stage, size of the displayed lymph nodes, and ps-Tg and TgAb levels could be used as sensitive indicators for predicting clinical outcomes.
The three-dimensional printed template guided technique for S2AI screw placement and a comparison with freehand technique
Authors: Zhou Zhenhai , Zeng Zhimin , Yu Honggui , Xiong Jiachao , Liu Zhiming , Zhou Rongping , Wan Wenbing , Pan Zhimin , Chen Lu , Cao Kai 
Published: March 2020

Abstract
Background Sacropelvic fixation continues to present challenges when involved in the adult spinal deformity correction. The S2 alar iliac (S2AI) fixation is commonly used in sacropelvic fixation. Several techniques, including intraoperative navigation and freehand technique, were used for S2AI screws placement. The aim of this study is to analyze the anatomic parameters for S2AI screw trajectory in Asian population and introduce a novel technique described as three-dimensional printed template guided technique (TGT). Meanwhile, the accuracy and safety of this technique were compared with conventional freehand technique. Methods The S2AI trajectory parameters were measured in100 Asian adult volunteers. Parameters were compared between different genders. Forty-eight adult patients who underwent S2AI screw placement were reviewed, 28 patients received freehand technique and 20 patients received TGT technique. Postoperative CT was used to assess the accuracy of screw trajectory and cortex violation related complications were recorded. Results The cephalocaudal angles (CA), maximal length of screw pathway (ML), narrowest width of pathway within the iliar teardrop (NW), distance from the center of teardrop to sciatic notch(SD) and distance of the start point distal to S1 dorsal foramen(DD) showed significant gender-related difference (p<0.05). All 48 patients were placed S2AI screws bilaterally (40 screws in TGT vs 56 screws in freehand). One screw penetrated iliac cortex in TGT group but 10 screws penetrated iliac cortex in freehand group (3% vs 17.9%) (p<0.05). Conclusion Approximately 30-35° of cephalocaudal angle and 39°mediolateral angle are appropriate for S2AI screw placement in Asian patients. Either freehand or TGT technique is safe for S2AI screw placement. TGT technique is more accurate compared with conventional freehand technique. Trial registration This is a retrospective study. Key words Sacropelvic fixation, Second sacral alar iliac (S2AI) screw, three-dimensional printed template, freehand technique
Anatomical Variation of the Psoas Valley: A Scoping Review
Authors: Kuroda Yuichi , Rai Ankit , Saito Masayoshi , Khanduja Vikas 
Published: March 2020

Abstract
Background: The aim of this scoping review was to investigate the literature on the anatomy of the psoas valley, an anterior depression on the acetabular rim, and propose a unified definition of the anatomical structure, describe its dimensions, anatomical variations and clinical implications. Methods: A systematic computer search of EMBASE, PubMed and Cochrane for literature related to the psoas valley was undertaken using Preferred Reporting Items for Systematic Reviews and Meta-analyses (PRISMA) guidelines. Clinical outcome studies, prospective/retrospective case series, case reports and review articles that described the psoas valley and its synonyms were included. Studies on animals as well as book chapters were excluded. Results: Of the 313 articles, the filtered literature search identified 14 papers describing the psoas valley and its synonyms such as iliopsoas notch, notch between anterior inferior iliac spine and the iliopubic eminence, Psoas-U and anterior wall depression. Most of these were cross-sectional studies that mainly analysed normal skeletal hips. In terms of anatomical variation, 4 different configurations of the anterior acetabular rim have been identified and it was found that the curved type was the most frequent while the straight type may be nonexistent. Additionally, the psoas valley tended to be deeper in males as compared with females. Several papers established the psoas valley, or Psoas-U in a consistent location at approximately the 3 o'clock on the acetabular rim which may have implications with labral pathology. Conclusion: This review highlights the importance of the anatomy of the psoas valley which is a consistent bony landmark. The anatomy and the anatomical variations of the psoas valley need to be well-appreciated by surgeons involved in the management of young adults with hip pathology and also joint replacement surgeons to ensure appropriate seating of the acetabular component.