bluuebunny/crossref_metadata_2025_split huggingface dataset:
Long non-coding RNA MIR4300HG polymorphisms are associated with postoperative nausea and vomiting: a genome-wide association study
Authors: Sugino Shigekazu , Konno Daisuke , Kawai Yosuke , Nagasaki Masao , Endo Yasuhiro , Hayase Tomo , Yamazaki-Higuchi Misako , Kumeta Yukihiro , Tachibana Shunsuke , Saito Katsuhiko , Suzuki Jun , Kido Kanta , Kurosawa Nahoko , Namiki Akiyoshi , Yamauchi Masanori 
Published: April 2020

Abstract
Background: Genetic factors such as single nucleotide polymorphisms (SNPs) play a key role in the development of postoperative nausea and vomiting (PONV). However, previous findings are not widely applicable to different populations because of population-specific genetic variation. We developed a Japanese-specific DNA microarray for high-throughput genotyping. The aim of the current study is to identify causal SNPs associated with PONV on a genome-wide scale using this microarray in a sample of Japanese surgical patients. Methods: Genomic DNA was obtained from 256 surgical patients. We first determined associations between 659,636 SNPs and the incidence of PONV 24hrs after surgery in a limited sample of 24 female patients using the microarray. After imputation of genotypes at 24,330,529 SNPs, 78 SNPs were found to be associated with the incidence of PONV. We chose 4 of the 78 SNPs to focus on by in silico functional annotation. Finally, we genotyped these 4 candidate SNPs in 255 patients using real-time PCR to verify association with the incidence of PONV. Results: The T > C variant of rs11232965 in the long non-coding RNA MIR4300HG was significantly associated with reduced incidence of PONV among genotypes and between alleles (p = 0.01 and 0.007). Conclusions: We identified a novel causal SNP (rs11232965) in the long non-coding RNA MIR4300HG that is associated with PONV. The rs11232965-SNP variant (T > C) is protective against the incidence of PONV. Trial registration: This study was registered at the UMIN Clinical Trials Registry (Identifier: UMIN000022903, date of registration: June 27th, 2016, retrospectively registered, https://upload.umin.ac.jp/cgi-open-bin/ctr_e/ctr_view.cgi?recptno=R000026392).
Leveraging Family Dynamics to Increase the Effectiveness of Incentives for Physical Activity: The FIT-FAM Randomized Controlled Trial
Authors: Finkelstein Eric , Lim Robyn Su May , Ward Dianne Stanton , Evenson Kelly R. 
Published: April 2020

Abstract
Background In Singapore, 37% of adults are insufficiently active despite widespread opportunities for physical activity (PA). Research shows that PA can be increased by use of group-based extrinsic incentives. This study evaluates whether a novel incentive design linking children’s incentives to both their own and their parent’s PA (measured via pedometers) can increase parents’ PA without compromising children’s PA. Methods We conducted a two-arm, parallel, open-labelled randomized controlled trial where parent-child dyads were randomly assigned to either (1) rewards to the child contingent on child’s PA (child-based) or (2) rewards to the child contingent on both child’s and parent’s PA (family-based). Parents had to be English-speaking, computer-literate, non-pregnant, full-time employees, aged 25–65 years, and with a participating child aged 7–11 years. Parent-child dyads were randomized within strata (self-reported low vs high weekly PA) into study arms in a 1:1 ratio. Participants were given pedometers to assess daily steps. The primary outcome was the between-arm difference in the change from baseline in parent’s mean steps/day measured by accelerometry at months 6 and 12. Results 159 and 157 parent-child dyads were randomized to the child-based or family-based arms, respectively, and all with outcomes evaluated an intent-to-treat perspective. At month 6, there was a statistically significant 613 steps/day (95% CI: 54–1171) differential in favour of family-based parents. At month 12, the differential was reduced to 369 steps/day (95% CI: -88–1114) and no longer statistically significant. Conclusions Our findings provide evidence that family-based incentive strategies have the potential to improve outcomes at no additional cost but also indicate that more research is needed to identify strategies that encourage long-term behavior change for both parents and children.
Upregulated Expression of LncRNA Nicotinamide Nucleotide Transhydrogenase Antisense RNA 1 is Correlated with Unfavorable Clinical Outcomes in Cancers
Authors: Zhang Chenghao , Ren Xiaolei , Liu Zhongyue , Tu Chao 
Published: April 2020

Abstract
Background: The nicotinamide nucleotide transhydrogenase antisense RNA 1 (NNT-AS1) is a long non-coding RNA aberrantly expressed in human malignancies. We aimed to analyze available data to evaluate the correlation between NNT-AS1 expression and cancer prognosis.Methods: Literature retrieval was performed by systematic searching related databases from inception to April 2, 2020. Studies regarding correlation between NNT-AS1 expression, survival outcomes and clinical characteristics of cancer patients were collected and pooled to calculate the the hazard ratios (HRs) or odds ratios (ORs) with 95% confidence intervals (95% CIs).Results: Ten studies comprising 690 patients were included. Overexpression of NNT-AS1 had a significant association with unfavorable overall survival (OS) (HR=2.08, 95% CI: 1.84-2.36, P<0.001). Stratified analysis showed that tumor type, sample size, follow-up months, and survival analysis approach did not change the predictive value of NNT-AS1 on OS. Furthermore, elevated NNT-AS1 level had significant association with distant metastasis (DM) (OR=2.45, 95% CI: 1.39-4.30), lymph node metastasis (LNM) (OR=3.92, 95% CI: 1.35-11.41), TNM stage (OR=4.25, 95% CI: 1.71-10.56), and vascular invasion (OR=3.98, 95% CI: 2.06-7.71), but was not associated with age and gender. The TCGA dataset showed the NNT-AS1 expression was strongly associated with poor OS, but not disease-free survival.Conclusions: high expression of NNT-AS1 could predict unfavorable survival and clinicopathologic outcomes, indicating NNT-AS1 may serve as a novel biomarker for prognosis and therapeutic target for patients.
The effectiveness of multi-component interventions targeting physical activity or sedentary behaviour amongst office workers: a 3-arm cluster randomised controlled trial
Authors: Nooijen Carla F. J. , Blom Victoria , Ekblom Örjan , Heiland Emerald G. , Larisch Lisa-Marie , Bojsen-Møller Emil , Ekblom Maria M. , Kallings Lena V 
Published: April 2020

Abstract
Background: Interventions to increase physical activity or reduce sedentary behaviour within the workplace setting have shown mixed effects. This cluster randomised controlled trial assessed whether multi-component interventions, focusing on changes at the individual, environmental, and organisational levels, either increased physical activity or reduced sedentary behaviour, compared to a passive control group.Methods: Teams of office-workers from two companies participated in two interventions (iPA: targeting physical activity; iSED: targeting sedentary behaviour), or a waiting list control group. Exclusion criteria was a very high physical activity level. Randomisation was done on a cluster level, and groups were randomly allocated (1:1) with stratification for company and cluster size Personnel involved in data collection and processing were blinded for group allocation. Both interventions included five sessions of cognitive behavioural therapy counselling for 6-months. iPA included counselling focused on physical activity, access to a gym, and encouragement to exercise and go for lunch walks. iSED included counselling on sedentary behaviour and encouragement to reduce sitting and increase engagement in standing- and walking-meetings. At baseline and the 6-month mark accelerometers were worn on the hip and thigh for 7 days. The primary outcomes were group differences in time spent in moderate-to-vigorous physical activity (%MVPA) and in sedentary behaviour (%), analysed for those with complete data using Bayesian multilevel modelling.Results: 263 office-workers (73% women, mean age 42±9 years, education 15±2 years) in 23 cluster teams were randomised (iPA n=84, 8 clusters; iSED n=87, 7 clusters; C n=92, 7 clusters). No significant group differences (posterior mean ratios: 95% credible interval) were found after the intervention for %MVPA or for %Sedentary. %MVPA: iPA vs C (0·04: -0·80 – 0·82); iSED vs C (0·47: -0·41 – 1·32); iPA vs iSED (0·43: -0·42 – 1·27). %Sedentary: iPA vs C (1·16: -1·66 – 4·02); iSED vs C (-0·44: -3·50 – 2·64); iPA vs iSED (-1·60: -4·72 – 1·47).Conclusions: The multi-component interventions focusing on either physical activity or sedentary behaviour were unsuccessful at increasing device-measured physical activity or reducing sedentary behaviour compared to control.Trial registration: ISRCTN92968402
Herd-level risk factors for chronic pleuritis in finishing pigs: a case-control study
Authors: Hälli Outi , Haimi-Hakala Minna , Oliviero Claudio , Heinonen Mari 
Published: April 2020

Abstract
Background Chronic pleuritis is a common finding in slaughtered pigs in post-mortem meat inspection. The prevalence of pleuritis has been increasing during the last decade also in Finland. The aim of this prospective case-control study was to search for environmental, infectious and management-related herd-level risk factors for pleuritis in the slaughterhouse. Altogether 46 Finnish pig herds, including 25 control (low pleuritis prevalence in meat inspection) and 21 case (high pleuritis) herds, were enrolled in the study and visited during the tenth week of the rearing period of finishing pigs. Herd personnel were asked about basic herd information, management and environmental factors. Selected pigs were examined clinically, environmental parameters were measured and 15 blood samples per herd were taken during herd visits. Antibodies against Actinobacillus pleuropneumonia serotype 2 (APP2) and ApxIV toxin and swine influenza virus were measured. After the slaughter of study pigs, meat inspection results of the batch were gathered from slaughterhouses. Multivariate logistic regression models were built to identify possible risk factors for a herd to be a case herd ( i.e. having high pleuritis values).Results Finishing herd type, herd size and APP2 seropositivity were observed to act as risk factors. In addition to these, general herd-level factors, flank biting and high APP2 antibody prevalence of the herd tended to be associated with the risk of the herd being a case herd. None of the other clinical signs of pigs, management-related factors or environmental measurements were associated with herd status.Conclusions As previously known, in endemic and subclinical infections such as APP, herd and management-related factors are important in building up infection pressure, but single risk factors seem to be difficult to identify. However, as flank biting was more common in high pleuritis herds, part of disease susceptibility is likely mediated via stress.
The clinical, immunological and genetic features of 12 Chinese patients with STAT3 mutation
Authors: Lin Li , Wang Ying , Sun Bijun , Liu Luyao , Ying Wenjing , Wang Wenjie , Zhou Qinhua , Hou Jia , Yao Haili , Sun Jinqiao , Wang Xiaochuan 
Published: April 2020

Abstract
Background: Loss-of-function (LOF) mutation in signal transducer and activator of transcription 3 (STAT3) was one of the causes of the hyper immunoglobulin E (IgE) syndrome (HIES), while gain-of-function mutation (GOF) in STAT3 leads to immune dysregulation diseases. We retrospectively report 11 LOF STAT3 patients and 1 GOF STAT3 patient and illustrate their onset age, common clinical symptoms, immunologic and molecular manifestation.

Methods: 12 patients were enrolled in our study. Serum immunoglobulins, lymphocyte subset detection and whole-exome sequencing were performed.

Results: The median onset age of STAT3-deficient patients was 1.89 years. Eczema, recurrent respiratory infection, apparent fever, abscesses and Staphylococcus aureus infection was the classical manifestation. Elevated serum IgE level is not entirely unanimous with high eosinophils counts. Moderate viral DNA was also measured in peripheral blood mononuclear cells. We noticed that c. 1144C>T was the most common spot, followed by c.1311C>A. Additionally, c.1311C>A and c. 1826G>C are two novel mutations. Eight patients obtained a notable improvement after received intravenous immunoglobulin (IVIG).

Conclusion: We believe IVIG may help reduce the opportunity of infection in STAT3-deficient patients. Significant variance at onset age probably is a great challenge for clinicians and urgently needs early diagnosis and treatment.
The role of tumor infiltrating lymphocytes (TILs) and the ratios between different subsets serve as prognostic factors in hypopharyngeal squamous cell carcinoma
Authors: Wang Jie , Tian Shu , Sun Ji , Zhang Jiahao , Lin Lan , hu chunyan 
Published: April 2020

Abstract
Background: Cancer cells induce the infiltration of various immune cells located or distributed in different sites and playing multiple roles, which have recently been proposed to predict clinical outcomes. We therefore studied the prognostic significance based on the presence of tumor infiltrating lymphocytes (TILs) and the ratios between different types of immune cells in hypopharyngeal squamous cell carcinoma (HPSCC).Methods: We retrospectively analysed 132 consecutive patients diagnosed with HPSCC in 2013-2017. Tumoral parenchyma were immunohistochemically counted manually for the number of CD8, CD4 and Foxp3. The ratios of CD8/Foxp3 and CD4/CD8 were calculated for each specimen, and analyzed with respect to patient clinicopathologic variables and prognosis. Results: HPSCC patients with high levels of TILs showed evidently correlations with well differentiated tumors (P < 0.05). Increased Foxp3+ TIL is also significantly associated with Stage and T stage (P =0.048 and P= 0.046, respectively). Kaplan-Meier analysis showed that high CD8 and FoxP3 infiltration correlated with favorable overall survival (OS, P = 0.019 and P = 0.001), disease-free survival (DFS, P = 0.045 and P = 0.028) and distant metastasis-free survival (DMFS, P = 0.034 and P = 0.009), respectively, but only FoxP3 displayed prognostic significance for DMFS in multivariate analysis (MVA). In lymphocyte ratios analysis, CD8/FoxP3 appeared to play a pivotal role, patients with high CD8/FoxP3 ratio had a superior 3-year DFS and DMFS compared with the low value of this parameter in both univariate analysis (UVA) and MVA (P = 0.015 and P=0.001). Meanwhile, high CD4/CD8 ratio had significantly better DFS and local relapse-free survival (LRFS) in UVA, and exhibited an independent prognostic factor for improved LRFS in MVA (P = 0.040).Conclusion: Although high TILs were determined to be prognostically significant in HPSCC, the ratios of these subsets may be more informative. Particularly, higher ratio of CD8/Foxp3 accurately predict prognosis for improved DFS and DMFS, and increment of CD4/CD8 ratio was an independent predictor for favorable LRFS.
Intervention fidelity and its determinants of focused antenatal care package implementation, in South Wollo, Northeast Ethiopia.
Authors: Tessema Asressie Molla , Gebeyehu Abebaw , Mekonnen Solomon , Alemu Kassahun , Tigabu Zemene 
Published: April 2020

Abstract
Introduction Evidences show that intervention packages at home-community setting are found to reduce neonatal mortality significantly. But millions of neonates are still dying by preventable causes with the implementation of these effective interventions. This might be due to evidence-practice gap. Therefore, this study aimed at assessing the intervention fidelity and factors influencing focused antenatal care package. Methods Cross-sectional study design was employed in this study. In the selected kebeles’, a total of 898 mothers who gave birth within six months before data collection, ten health posts with their health extension workers were employed. Interview-administered, self-administered and facility audit checklist were used for collecting data from all mothers, health extension workers and health posts respectively. Mothers were asked whether or not the required level of care were provided to them. Health extension workers & health posts were assessed for the competency and organizational drivers. Multilevel linear regression model were used to identify the possible moderating factors at individual and organizational level for intervention fidelity. Result The overall weighted average focused antenatal care package intervention fidelity was 49.78% (95% CI: 47.73 – 51.83). The weighted average focused antenatal care package intervention fidelity by health extension workers was 62.02% (95% CI: 59.71 – 64.32) and skilled providers were 56.57% (95% CI: 53.94 – 59.19). The overall antenatal care coverage were 83.7% (95% CI: 81.28 – 86.12) with 34.97% (95% CI: of 31.56 – 38.39) of mothers received at least four antenatal visit and 6.12% (95% CI: 4.40-7.83) of them received all the recommended components of focused antenatal care. Previous maternal problem, paternal education and implementation of supportive/facilitation strategy were significant facilitators for focused antenatal care package intervention fidelity. Conclusion In this study, focused antenatal care package intervention fidelity was low. This implies that the stagnation of neonatal mortality reduction might be partly due to the low focused antenatal care packages intervention fidelity. Paternal education and implementation of supportive/facilitation strategies were statistically significant facilitators of focused antenatal care intervention package fidelity. Therefore, improvement of implementation of facilitation strategy is recommended.
Extracts of Rhodiola rosea L. and Scutellaria galericulata L. in functional dairy products
Authors: Zaushintsena Alexandra , Bruhachev Evgeny , Belashova Olga , Asyakina Lyudmila , Kurbanova Marina , Vesnina Anna , Fotina Natalya 
Published: April 2020

Introduction. Modern scientific research into the biochemical composition and medicinal value of plants makes it possible to use them as functional ingredients in food technology. The research objective was to test rose root (Rhodiola rosea L.) and scullcap (Scutellaria galericulata L.) for biologically active substances and their potential use in functional dairy products.
Study objects and methods. The research featured biologically active substances (BAS) obtained from rose root and scullcap that grow in mountain areas or on rock outcrops along Siberian rivers. The BAS content was determined using high performance liquid chromatography (HPLC). The biologically active substances were screened and identified using HPLC, thin-layer chromatography (TLC), and infra-red identification (IR). The new functional products were based on whey and cottage cheese made from processed whole milk.
Results and discussion. The analysis of Rhodiola rosea rhizomes and roots showed the following BAS content (mg/g): rosavin – 16.9, salidroside – 14.3, rosin – 5.04, rosarin – 2.01, and methyl gallate – 6.8. The roots of Scutellaria galericulata had the following BAS content (mg/g): scutellarein – 22.27, baicalin – 34.37, baicalein – 16.30, apigenin – 18.80, chrysin – 6.50, luteolin – 5.40, and vogonin – 3.60. Whey served as a basis for a new functional whey drink fortified with BAS isolated from Rhodiola rosea 100 mL of the drink included 50 mL of whey, 20 mL of apple juice, 0.1 mL of rose root concentrate, 3 g of sugar, 0.5 g of apple pectin, 04 g of citric acid, and 30 mL of ionized water. The content of phytochemical elements ranged from 0.11 ± 0.001 to 0.49 ± 0.08 mg/100 g. Cottage cheese served as a basis for another dairy product fortified with BAS obtained from Scutellaria galericulata. The formulation included 81 g of cottage cheese, 10 mL of cherry jam, 9 g of sugar, and 0.025 mL of scullcap concentrate. The content of biologically active substances in the finished product varied from 0.09 ± 0.02 for luteolin to 0.48 ± 0.11 for baicalin. The whey drink fortified with the BAS extracted from Rhodiola rosea and the cottage cheese product fortified with the BAS isolated from Scutellaria galericulata satisfied 40–45% and 55–60% of the reference daily intake for phenolic compounds, respectively. The obtained data made it possible to recommend the new functional foods for commercial production.
Conclusion. A set of experiments was performed to isolate biologically active substances from Rhodiola rosea and Scutellaria galericulata. The research developed and tested formulations of two new functional products based on whey and cottage cheese.
Antioxidant activity of aqueous and alcohol extracts of Salvia leriifolia L. and Linum usitalissmum L. subjected to a pulsed electric field
Authors: Shirazi Shohreh Arab , Nia Ahmad Pedram , Asl Mohammad Reza Saeidi , Naghipour Fariba , Tavakolipour Hamid 
Published: April 2020

Introduction. More attention has been paid in recent decades to extracts and essential oils from various plants as natural antioxidant sources due to their positive effects on food oxidation reactions. Our study aimed to compare the antioxidant activity of aqueous and alcoholic extracts from Salvia leriifolia L. and Linum usitalissmum L.The extracts were subjected to a pulsed electric field with intensities of zero (without pretreatment), 3 and 6 kV·cm–1, and a constant pulse number of 30. For this purpose, parameters such as total phenolic compounds and antioxidant activity were investigated by DPPH and TEAC methods.
Results and discussion. Our results showed that a higher intensity of a pulsed electric field pretreatment and the use of an alcoholic solvent significantly raised total phenolic compounds in the extracts and their antioxidant activity at a 95% confidence level. We found significant effects of the plant source (Linum usitalissmum and Salvia leriifolia), pretreatment (pulse electric field at intensities of 0.3 and 6 kV·cm–1), and a solvent (aqueous and alcohol) on the extracts’ antioxidant activity (P < 0.05). In addition, there was a significant correlation between the results of the DPPH and the TEAC antioxidant activities (P < 0.01 and r = 0.932).
Conclusion. The total antioxidant activity (based on both TEAC and DPPH methods) and total phenolic compounds extracted from Salvia leriifolia were higher than those from Linum usitalissmum (P < 0.05). Based on the results, the extract obtained from Salvia leriifolia with an alcoholic solvent and a pulsed electric field pretreatment (at 6 kV·cm–1 and 30 pulses) was selected as possessing desired antioxidant properties.
Effect of treated sewage characteristics on duckweed biomass production and microbial communities
Authors: Iwano Hiroshi , Hatohara Syo , Tagawa Tadashi , Tamaki Hideyuki , Li Yu-You , Kubota Kengo 
Published: April 2020

Abstract
Duckweed biomass production in a duckweed pond fed with three differently treated sewage (i.e. sewage treated by primary sedimentation (PS); conventional activated sludge process (CAS); and downflow hanging sponge process (DHS)) was evaluated in order to assess the effects of water quality on biomass yield. Higher and stable biomass production was observed when the duckweed pond was fed with PS or DHS-effluent than with CAS-effluent, evidently due to the difference in nutrient loads. Availability of nutrients, especially phosphorus, affected the biomass production rate: higher the nutrient, faster the production. Microbial community analysis revealed that the members of Rhizobiales were likely to contribute to stable and high biomass growth. From the results of the study, a sewage treatment system consisting of a primary sedimentation followed by a duckweed pond and a tertiary treatment unit can be proposed to maximize biomass production without compromising treatment objectives. Size and operational parameters of the duckweed pond should be determined primarily based on the nutrient availability in the influent water to maximize duckweed growth.