bluuebunny/crossref_metadata_2025_split huggingface dataset:
WITHDRAWN: Mig-6 Promotes the Apoptosis and Inhibits of the Flux of Autophagy in HCC Cell Lines Through Modulating Mig-6/miR-193a-3p/TGF-β2 Axis
Authors: Li Zixuan , Tian Yulong , Hong Duo , Wang Fan , Zhong Hongshan 
Published: July 2020

Abstract
Background:Mitogen-inducible gene 6 (Mig-6) is a tumor suppressor gene that plays an important role in many types of cancers by interacting with EGFR. Thus far, little is known about the molecular mechanism of Mig-6 in hepatocellular carcinoma (HCC). Also, the relationship between Mig-6 and miRNAs needs to be elucidated. Therefore, this study first aimed to find whether Mig-6 could promote apoptosis and inhibition of the flux of autophagy by its downstream miRNA in HCC cell lines. Methods: Two cell lines, HepG2and HLE, were used in this study. Mig-6 overexpression and knockdown models , miR-193a mimics and inhibitors models,were established. MiRNA microarray profiling were used to verified Mig-6 regulated- miRNA. Real-time PCR, Western blot analysis were carried out to detect RNA and protein expression.Evaluation of fluorescent LC3 puncta and cell fow cytometer assay were used to test the autophagy and apoptosis respectively. Results: Mig-6 induced the apoptosis and reduced the autophagy of HCC cell lines. MiR-193a-3p is a Mig-6-regulated miRNA in Mig-6 overexpression model, and miR-193a-3p affected the apoptosis and autophagy of HCC cells by regulating the expression of TGF-β2. Additionally, the relationship between Mig-6 and transforming growth factor TGF-β2 was explored in depth for the first time.Conclusion:These findings revealed an important regulatory axis Mig-6/miR-193a-3p/TGF-β2 in the apoptosis and autophagy of HCC cells, providing a novel insight into the therapeutic target in HCC.
Impact of Educational Self-efficacy Enhancement Program on Quality of Life, Pain Management, Self-efficiency Behaviors, and Its Impact of Satisfaction Among Adult Diabetic Patients With Peripheral Neuropathy Pain
Authors: Abu-Shennar Jawad.A AHMAD , Bayraktar Nurhan Bayraktar 
Published: July 2020

Abstract
Aims: This study aims to investigate the impact of the educational self-efficacy enhancement program on quality of life, pain management, self-efficiency behaviors, and its impact satisfaction among adult diabetic patients with peripheral neuropathy pain.Materials and methods: This randomized controlled study was conducted at the Jordanian Ministry of Health. during the period from October - March 2020 with 72 adult diabetic patients with peripheral neuropathy pain, after the ethical approval. Study group participants have implemented an educational self-efficacy enhancement program.Demographic data from, Diabetes Self-Efficacy Scale, Quality of Life Questionnaire(EQ-5D), Pain Intensity Scale, and Patient Satisfaction Questionnaire were used in data collection. Results: This study found that the self-efficacy enhancement program had a significantly positive impact on diabetic peripheral neuropathy pain patients’ QoL, pain levels, self-efficiency behaviors, and satisfaction. Conclusion:It is crucial to improve their self-efficacy behaviors and modification of lifestyle and behavioral changes such as appropriate diet, exercise, reduce weight and encouragement stop smoking, and regularly visit treating physicians.
COVID-19: The Changing Trend of Tele-consultations in a Private Tertiary Level Health Facility in Tanzania
Authors: Adebayo Philip B , Jusabani Ahmed Mussa , Mukhtar Murtaza , Zehri Ali Akbar 
Published: July 2020

Abstract
Introduction: The current COVID-19 pandemic has occasioned the increased adoption of telemedicine. This study reports the uptake and trend of a new teleconsultation service in a Tanzanian hospital.Methods: This is a retrospective observational study that profiled requests for teleconsultations and uptake of the service between April 1, 2020, and June 30, 2020.Results: Two hundred and eighteen telephone inquiries were received over the 3 months. One hundred and sixteen (53.2%) individuals followed through with the teleconsultations. Paediatric (38.8%) and Internal medicine (32.8%) were the subspecialties with the highest number of teleconsultations. In a frame of 3 months, teleconsultation uptake was highest in May and lowest in June.Conclusion: There was a steady rise and a rapid fall in requests and uptake of teleconsultation services over the period under evaluation. Lack of insurance coverage for teleconsultations was a significant barrier. We propose a re-education and reiteration of the benefits of telemedicine to all stakeholders. This is important for the current era and beyond.
Body Fat Distribution Measured by Bioelectrical Impedance Analysis is Associated with Occurrence of Colorectal Adenoma: A Case Control Study
Authors: Liu Zhong-Hui , Zhang Gui-Xi , Zhang Hao , Jiang Li , Deng Yang , Liu Jian-Wen , Yang Xue-Fei , Chan Fion Siu-Yin , Fan Joe King-Man 
Published: July 2020

Abstract
ObjectivesSome studies have reported that visceral fat volume measured by computed tomography (CT) was a risk factor for colorectal adenoma (CRA). However, CT is not a good method for risk stratification in clinical use for its radiation. Bioelectrical impedance analysis (BIA) is a non-invasive method for assessment of body composition. The aim of current study was to evaluate the association of body fat measured by BIA with occurrence of CRA.Patients and methods:A total of 410 participants who had underwent screening colonoscopy from Jul 2017 to Dec 2019 in our center were recruited, including 230 with adenoma and 180 without adenoma detected. Body fat of participants were measured by BIA, including body fat mass (BFM), body fat percent (BFP), and waist-to-hip fat ratio. Parameters of metabolic syndrome (MetS) were also measured, including waist circumference, blood pressure, fasting blood glucose, blood level of triglyceride, cholesterol and high-density lipoprotein.ResultsAccording to univariate analysis, age, male proportion, BMI, waist circumference, body fat mass, waist-to-hip fat ratio, blood pressure, and FBG were higher in adenoma group than adenoma-free group, p < 0.05. On multivariate analysis (adjusted for age, sex, smoking, drinking, family history of CRC, etc.), High waist-to-hip fat ratio measured by BIA was associated with occurrence of CRA (compared with normal waist-to-hip fat ratio, OR 1.84; 95% CI, 1.09–3.09; p = 0.02). High waist circumference, as a component of MetS, was also independently associated with CRA (OR 1.90; 95% CI 1.17–3.08, p = 0.01).ConclusionBody fat distribution measured by BIA is associated with occurrence of CRA. Central obesity is a risk factor for CRA.Trial registration: This study was prospectively registered in Chinese Clinical Trials Registry (www.chictr.org.cn; registry number: ChiCTR-RRC-17010862).
Amyloid-β Protein and Neuroglobin Protein as Biomarkers on Brainstem Following Traumatic Brain Injury in Rats
Authors: Li Wenhe , Zhu Haijun , Liang Yue , Tong Fang , Zhou Yiwu 
Published: July 2020

Abstract
Background: Biomarkers play an important role in accurate diagnosis of traumatic brain injury (TBI). Due to the complexity and diversity of TBI, it is likely that a single biomarker will not be used for exactly diagnose. Amyloid-beta (Aβ) protein is generated by sequential cleavage of amyloid precursor protein (APP) by β- and γ-secretase, which may exert its toxic effects by increasing reactive oxygen species and neuroinflammation in the brain as damage factor of TBI. Its use in diagnosis for TBI is becoming more widespread. Neuroglobin (NGB) protein is great potential to diminish neuronal damage. Most epidemiological evidence suggested that Aβ and NGB may be used as biomarkers on brainstem (BS) following TBI. The aim of this study was to investigate the trend of Aβ and NGB on BS of rats with TBI and to analyze comprehensively them as potential biomarkers. Methods: Adult male Sprague-Dawley rats were subjected to the modified weight-drop model of closed TBI. Biologic behavior observation, histopathological assessments and western blot assay were performed. Aβ and NGB expression indicated temporal changes in BS after TBI. Their accuracy and efficiency of performing these tasks were calculated and statistical comparisons performed.Results: The results of Aβ enable us to speculate that the time points of 3 h, 6 h and 12 h may be crucial points for the diagnosis of TBI. NGB expression in the injured had obvious difference versus the control, the points of 1 h and 3 h were apparently higher than the control, and the groups of 12 h and 48 h were two peaks in the present study. Furthermore, the immunofluorescence assay results supported that Aβ and NGB co-localization in the neuros of BS, and the NGB specific expression in the BS of neurons.Conclusions: Therefore, the expression and change rules of Aβ and NBG in the BS may provide an important foundation for the diagnosis of TBI, damage assessment and therapeutic intervention.
Analysis of the interconnection of the GSTP1, CYP1A2, CYP1A1 genes in children with congenital heart diseases
Authors: Tsepokina A. V. , Ponasenko A. V. , Shabaldin A. V. 
Published: July 2020

The article presents data on the study of gene interconnections between the xenobiotics detoxification genes in various phases.Materials and methods.The study involves 131 children with congenital heart diseases (CHD) and 103 conditionally healthy children. The genotyping was performed by RT-PCR method using TaqMan probes. Intergenic bonds were analyzed via MDR v.3.0.2.Results and conclusion.We discovered no statistically significant differences in the genotype distribution in children with CHD and conditionally healthy donors. The analysis of intergenic interactions helped to develop a five-locus model characterized by the highest reproducibility, sensitivity and specificity: GSTP1 rs1793068 – GSTP1 rs6591256 – GSTP1 rs1871042 – CYP1A1 rs1048943 – CYP1A2 rs762551. This model was used to determine a number of protective and risky combinations of congenital heart defects-associated genotypes in children.
Diagnostic value of reticulocyte hemoglobin equivalent to confirm iron deficiency in full-term infants
Authors: Balashova E. A. , Mazur L. I. , Persteneva N. P. 
Published: July 2020

Diagnostics of Iron deficiency anemia (IDA) in outpatient pediatric practice is often based on decreased hemoglobin level. Latent iron deficiency diagnostic is not a part of current routine practice.Objective. To study the diagnostic value of red blood cell indices and reticulocyte hemoglobin equivalent in diagnostics of iron deficiency in full-term infants.Children characteristics and research methods. A prospective cohort study of healthy full-term children aged from 6 to 12 months at the children hospitals of Samara and Tolyatti. The laboratory examination included a general blood test to determine the concentration of hemoglobin, the number of red blood cells, red blood cell indices, and reticulocyte hemoglobin equivalent (Ret-He); to determine serum ferritin and C-reactive protein. The AUC (area under the curve) was used to determine the diagnostic value of quantitative indicators. The children with anemia without iron deficiency and children who received iron supplements within 1 month prior to laboratory examination were excluded from the analysis.Results. The study involved 207 children. When diagnosing iron deficiency in children, the highest AUC was found in Ret-He: 0.747 [0.679; 0.816] in 6-months-old children and 0.790 [0.708; 0.871] in 1-year-old children. The Ret-He diagnostic value was higher in children with iron deficiency: AUC 0.826 [0.754; 0.898] in 6- months-old children and 0.865 [0.809; 0.920] in 1-year-old children.Conclusion. Ret-He is a better predictor of iron deficiency in children under 1 year as compared to the red blood cell indices. The diagnostic value of red blood cell indices and Ret-He is higher in case of iron deficiency anemia than in case of iron deficiency conditions.
Causes, frequency and avoidance of diagnostic errors in newborns and children of the first year of life
Authors: Kirilochev O. K. 
Published: July 2020

The article presents research methods to detect the frequency of diagnostic errors.Objective: to compare clinical and pathological diagnoses in order to determine the frequency, causes and ways of avoiding diagnostic errors in children with infectious pathology specific to the perinatal period. The authors studied 234 death cases in the intensive care unit for newborns in 2006–2018, and they found diagnostic errors in 18,3% of cases. 53,4% of the diagnostic errors were associated with unrecognized infectious diseases specific to the perinatal period. The authors found that the correct intravital diagnosis was impossible for objective reasons in 65% of cases. Those objective reasons were mainly caused by diagnostic difficulties due to the lack of characteristic clinical data or the atypical course. Almost in every third patient the diagnostic errors were caused by subjective reasons and were associated with the diagnosis of congenital cytomegalovirus infection and neonatal sepsis. The subjective errors were often caused by the so-called doctor’s bona fide delusion due to a lack of knowledge, skills, experience. As the judgment error was the most common reason for misdiagnosis we need to improve the clinicians’ cognitive condition. Based on the audit results, the authors proposed the additional diagnostic approaches for certain diseases. One way to reduce errors is to improve educational initiatives for doctors.
Assessment of exposure of children swimmers to trihalomethanes in an indoor swimming pool
Authors: Wang Xiaoshuang , Dong Shaoxia 
Published: July 2020

Abstract
This study aimed to understand the exposure levels of trihalomethanes (THMs) in an indoor swimming pool and calculate the risks of exposure to THMs, based on the presence of each THM species, of children swimmers aged 6–17, in Beijing, China. We obtained exposure factors for the children through questionnaires and measured THM concentrations through laboratory tests, and we combined the results with an exposure model to calculate the risks, with consideration of different exposure routes (oral ingestion, inhalation and dermal absorption). In terms of exposure factors for the swimmers aged 6–17, the average body weight, exposure duration, exposure frequency, swimming time, shower time, changing time, warm-up exercise and rest time, skin surface area and ingestion rate of pool water were 40.46 kg, 2.70 years, 96 events/year, 64.03 min/event, 17.04 min/event, 15.31 min/event, 12.71 min/event, 1.37 m2 and 48.93 ml/event, respectively. The THM concentrations in swimming pool water, shower water, swimming pool air and locker room air were 67.17 μg/L, 12.64 μg/L, 358.66 μg/m3 and 40.98 μg/m3, respectively. The average cancer risk of THMs was 5.44 × 10−6, which is an unacceptable risk according to the United State Environmental Protection Agency (USEPA) Guidelines. The average hazard index was 0.007, i.e., less than 1, indicating that the noncancer risk was acceptable. Chloroform (TCM) was the main substance in four species of THMs and inhalation exposure was the main exposure pathway. The risk of cancer and noncancer from inhalation exposure to THMs accounts for 97–99% of the total risk. As a result, the disease control authorities and administrative department should pay attention to the health and safety of swimming facilities and, at the same time, establish standards for THMs in the air through further research.
PRESCRIPTION ANALYSIS, DRUG INTERACTION CHECKING AND IMPACT OF PATIENT COUNSELING BY A CLINICAL PHARMACIST IN TYPE II DIABETES MELLITUS PATIENTS IN A TERTIARY CARE HOSPITAL-A PROSPECTIVE STUDY
Authors: KRISHNARAJAN D. , RANJINI B. SHIVA , M. S. ARYA , SUNNY SMINU 
Published: July 2020

Objective: To evaluate the clinical pharmacist role in the prescription analysis, drug interaction and the impact of patient counseling in type 2 diabetes mellitus patients.
Methods: A prospective study was conducted in 203 type II Diabetes Mellitus patients for a period of 6 mo in the Diabetology department. Prescriptions were analyzed and self-care assessment for good health practices were collected using a questionnaire and the adherence scores were calculated. Patient counseling was provided to the patient and a follow up was done using the same self care assessment questionnaire.
Results: Out of 203 patients, 86 multiple therapy, 68 dual therapy and 49 monotherapy were observed. Glimepiride+Metformin (54), a combination of short acting and intermediate-acting insulin (41) was the most commonly prescribed drugs. Out of 1102 drugs, 488 were anti diabetic drugs, 35 were antibiotics, 579 were other drug classes prescribed. The drug interactions were reported. The adherence score shows a highly significant impact after counseling.
Conclusion: The results of the current study helps to understand the changes in prescription pattern, drug interactions and the impact of patient counseling by a clinical pharmacist.
Pengelolaan Obat Dengan E-Purchasing Untuk Pasien Program Rujuk Balik Di Fasilitas Kesehatan Tingkat Pertama
Authors: Maryani Herti , Kristiana Lusi , Andarwati Pramita , Paramita Astridya , Aimanah Ira Ummu 
Published: July 2020

PRB is a health service provided to people with chronic diseases. The implementation of PRB has been runningsince 2014, but until now it is still not optimal, one of which is the procurement and availability of medicines. The aim of the study was to study drug management for PRB patients. The research was conducted in Surabaya 2018. This is descriptive research with cross-sectional design. Data collection by in-depth interviews with pharmacy department managers in two FKTP units and pharmacies in Surabaya. Data were analyzed descriptively. The results of the study show that FKTP doesn’t buy medicine with e-purchasing, because the drug is given by the pharmacy according to the BPJS mapping list. The pharmacy has many obstacles to ordering drugs with e-purchase, so the order is done conventionally. The pharmacy orders drugs in several ways using the Order Letter, calling PBF and ordering via the WhatsApp (WA) application. The Guidelines for Procurement of Medicines with E-Purchasing Procedures Based on E-Catalogs already exist, but socialization must continue to be carried out, especially at the level of Puskesmas and pharmacies. Periodic evaluations must be carried out so that problems and defi ciencies that occur in the fi eld can be immediately resolved.Cooperation and good intentions are needed between various parties so that all involved can benefi t from this program, especially PRB patients.
Abstrak
Program Rujuk Balik (PRB) adalah pelayanan kesehatan yang diberikan kepada penderita penyakit kronis. PRBsudah berjalan sejak tahun 2014, namun masih belum optimal, salah satunya perihal pengelolaan obat. Tujuan penelitian adalah mengkaji pengelolaan obat untuk pasien PRB. Penelitian dilakukan di Surabaya tahun 2018. Jenis penelitian adalah deskriptif dengan desain potong lintang. Pengumpulan data dilakukan dengan wawancara mendalam kepada pengelola bagian farmasi di 2 unit Puskesmas dan 2 Apotek di Surabaya. Data dianalisis secara deskriptif. Hasil penelitian menunjukkan bahwa Puskesmas tidak melakukan pengadaan obat secara e-purchasing, karena obat diberikan oleh apotek sesuai daftar mapping BPJS. Apotek mempunyai banyak kendala dalam melakukan pemesanan obat dengan e-purchase, sehingga pemesanan dilakukan secara konvensional. Apotek melakukan pemesanan obat dengan beberapa cara yaitu menggunakan Surat Pemesanan (SP), menelpon PBF (Perusahaan Besar Farmasi) dan melalui aplikasi WhatsApp (WA).Petunjuk pelaksanaan pengadaan obat dengan prosedur E-Purchasing, berdasarkan E-Catalogue, sudah ada namun sosialisasi harus terus dilakukan terutama di tingkat Puskesmas dan apotek. Evaluasi berkala harus dilakukan agar permasalahan dan kekurangan yang terjadi di lapangan dapat segera diselesaikan. Perlunya kerja sama dan komitmen antar berbagai pihak sehingga semua yang terlibat dapat merasakan manfaat akan program ini, terutama pasien PRB.
The Observing Learning Activity Assisted by Concrete Media Improves Student's Conceptual Knowledge
Authors: Tegeh I Made , Parwata I Gusti Lanang Agung , Ostaviani Bernadeth Grace 
Published: July 2020

The purpose of this study was to examine the effect of observing learning activities assisted by concrete media in a scientific approach toward the conceptual knowledge of the fifth grade students. This study was a quasi-experiment with a post-test only control group design. The population of this study was all students of the fifth grade of Public Elementary School (SDN) 4 Cempaga and Public Elementary School (SDN) 4 Kawan. The samples were taken by purposive sampling. Conceptual knowledge data were collected by using multiple choice tests. The obtained data were analyzed by using a t-test. Based on the results of the t-test analysis, it was obtained tcount = 11. 36 while the ttable value was 2. 021. Based on tcount = 11. 36> ttable = 2. 021 then H0 was rejected. The results of the analysis prove that there are significant differences between a group of students who are taught by observing learning activities assisted by concrete media and a group of students who are taught by conventional learning. In addition, the average value of the experimental group learning outcomes is X̅ = 22. 30 which is higher than the average learning outcomes of the control group X̅ = 18. 40. From the results of the t-test analysis and the average learning outcomes, it can be concluded that the observing learning activities assisted by concrete media influence the conceptual knowledge of the fifth grade students. Therefore, observing learning activities assisted by concrete media are suitable to be applied to overcome the low conceptual knowledge of students. This finding will have implications, namely, to improve conceptual knowledge can be done by observing activities assisted by concrete media so the learning activities will be more meaningful and interesting.
Karma Yoga Dalam Kepemimpinan Organisasi
Authors: Wididana Gede Ngurah 
Published: July 2020

Leadership determines the success of an organization in achieving organizational goals. Karma yoga is a service attitude practiced by everyone as a leader for himself and organization to provide the best service to others and organization. The application of karma yoga as the service attitude of life taught in the Bhagavad Gita is really important to be studied relevant to the leadership of organization in modern era. The servant leadership described by Greenleaf is closely related to karma yoga as a service attitude by leader in organization, which aims to serve sincerely by dedicating service to god. Karma yoga is depicted in Mahabharata as a patient and loving attitude, courageus and responsible, diligent and learner, honest and loyal. Karma yoga constitutes the basic for the servant leadership to realize organizational goals.
Pentingnya Laku Semedi Bagi Pemangku dan Pinandhita
Authors: Poniman Romo 
Published: July 2020

<p><em>The highest desirable behavior for the followers of Kejawen is, among others, exercise, exercise which in this case requires a routine training. Having a high mysticism can be more sensitive to feelings in overcoming and accepting conditions. But to have a high level of mysticism requires a special discipline of life and behavior to achieve this situation. In practicing self-discipline, a follower of Kejawen should often do Semedi. By meditation in this case it is a special behavior to train oneself to join oneself with Gusti. Before doing Semedi, you should realize the concept of the existence of the Three Realms in the human body. After knowing it, the Semedi training can be carried out to find the peak of success that will eventually be trained to have a sensitive and holy mind so that peace is felt.</em></p><p><em> </em></p><p><strong><em><br /></em></strong></p>