bluuebunny/crossref_metadata_2025_split huggingface dataset:
Controls on the Isotopic Composition of Nitrite (δ15N and δ18O) during Denitrification in Freshwater Sediments
Authors: Sebilo Mathieu , Aloisi Giovanni , Mayer Bernhard , Perrin Emilie , Vaury Véronique , Mothet Aurélie , Laverman Anniet M. 
Published: December 2019

AbstractThe microbial reduction of nitrate, via nitrite into gaseous di-nitrogen (denitrification) plays a major role in nitrogen removal from aquatic ecosystems. Natural abundance stable isotope measurements can reveal insights into the dynamics of production and consumption of nitrite during denitrification. In this study, batch experiments with environmental bacterial communities were used to investigate variations of concentrations and isotope compositions of both nitrite and nitrate under anoxic conditions. To this end, denitrification experiments were carried out with nitrite or nitrate as sole electron acceptors at two substrate levels respectively. For experiments with nitrate as substrate, where the intermediate compound nitrite is both substrate and product of denitrification, calculations of the extent of isotope fractionation were conducted using a non-steady state model capable of tracing chemical and isotope kinetics during denitrification. This study showed that nitrogen isotope fractionation was lower during the use of nitrite as substrate (ε = −4.2 and −4.5‰ for both treatments) as compared to experiments where nitrite was produced as an intermediate during nitrate reduction (ε = −10 and −15‰ for both treatments). This discrepancy might be due to isotopic fractionation within the membrane of denitrifiers. Moreover, our results confirmed previously observed rapid biotic oxygen isotope exchange between nitrite and water.
Experimental interference of uncorrelated photons
Authors: Kim Heonoh , Kwon Osung , Moon Han Seb 
Published: December 2019

AbstractThe distinguishing of the multiphoton quantum interference effect from the classical one forms one of the most important issues in modern quantum mechanics and experimental quantum optics. For a long time, the two-photon interference (TPI) of correlated photons has been recognized as a pure quantum effect that cannot be simulated with classical lights. In the meantime, experiments have been carried out to investigate the classical analogues of the TPI. In this study, we conduct TPI experiments with uncorrelated photons with different center frequencies from a luminescent light source, and we compare our results with the previous ones of correlated photons. The observed TPI fringe can be expressed in the form of three phase terms related to the individual single-photon and two-photon states, and the fringe pattern is strongly affected by the two single-photon-interference fringes and also by their visibilities. With the exception of essential differences such as valid and accidental coincidence events within a given resolving time and the two-photon spectral bandwidth, the interference phenomenon itself exhibits the same features for both correlated and uncorrelated photons in the single-photon counting regime.
The CC′ loop of IgV domains of the immune checkpoint receptors, plays a key role in receptor:ligand affinity modulation
Authors: Kundapura Shankar V. , Ramagopal Udupi A. 
Published: December 2019

AbstractAntibodies targeting negative regulators of immune checkpoints have shown unprecedented and durable response against variety of malignancies. While the concept of blocking the negative regulators of the immune checkpoints using mAbs appears to be an outstanding approach, their limited effect and several drawbacks, calls for the rational design of next generation of therapeutics. Soluble isoforms of the negative regulators of immune checkpoint pathways are expressed naturally and regulate immune responses. This suggests, affinity-modified versions of these self-molecules could be effective lead molecules for immunotherapy. To obtain better insights on the hotspot regions for modification, we have analysed structures of 18 immune receptor:ligand complexes containing the IgV domain. Interestingly, this analysis reveals that the CC′ loop of IgV domain, a loop which is distinct from CDRs of antibodies, plays a pivotal role in affinity modulation, which was previously not highlighted. It is noteworthy that a ~5-residue long CC′ loop in a ~120 residue protein makes significant number of hydrophobic and polar interactions with its cognate ligand. The post-interaction movement of CC′ loop to accommodate the incoming ligands, seems to provide additional affinity to the interactions. In silico replacement of the CC′ loop of TIGIT with that of Nectin-2 and PVR followed by protein docking trials suggests a key role of the CC′ loop in affinity modulation in the TIGIT/Nectin pathway. The CC′ loop appears to be a hotspot for the affinity modification without affecting the specificity to their cognate receptors.
Interrater and intrarater agreement and reliability of ratings made using the Zaidi–Dayal and Richards–Jabbour scales for the shape of the foramen magnum
Authors: Amarin Justin Z. , Alzraikat Sayel H. , Suradi Haya H. , Omari Rand Y. , Ghafel Afnan N. , Badran Darwish H. , Samara Osama A. 
Published: December 2019

AbstractAnatomists and radiologists use the Zaidi–Dayal and Richards–Jabbour scales to study the shape of the foramen magnum. Our aim is to measure the interrater and intrarater agreement and reliability of ratings made using the two scales. We invited 16 radiology residents to attend two sessions, four weeks apart. During each session, we asked the residents to classify the shape of the foramen magnum in 35 images using both scales. We used Fleiss’ κ to measure interrater reliability and Cohen’s κ to measure intrarater reliability. The interrater reliability of ratings made using the Zaidi–Dayal scale was 0.34 (0.26–0.46) for session one and 0.30 (0.24–0.39) for session two, and the intrarater reliability was 0.39 (0.34–0.44). The interrater reliability of ratings made using the Richards–Jabbour scale was 0.14 (0.10–0.19) for session one and 0.12 (0.09–0.17) for session two, and the intrarater reliability was 0.11 (0.07–0.15). In conclusion, the interrater and intrarater agreement and reliability of ratings made using the Zaidi–Dayal and Richards–Jabbour scales are inadequate. We recommend an objective method by Zdilla et al. to researchers interested in studying the shape of the foramen magnum.
Influence of age and gender on sex steroid receptors in rat masticatory muscles
Authors: Galhardo Alessandra Pucci Mantelli , Mukai Márcio Katsuyoshi , Mori Matsuyoshi , Carvalho Katia Candido , Baracat Maria Cândida Pinheiro , Simões Manuel de Jesus , Soares José Maria , Baracat Edmund Chada 
Published: December 2019

AbstractThe temporomandibular muscle dysfunction is characterized by myofascial pain and is more prevalent in women of reproductive age. Sex steroid hormones are hypothetically involved in the dysfunction, but few are the studies of steroid receptors in masticatory and mastication-related muscles. Our aim was to determine estrogen and testosterone receptor expression in rat masticatory and mastication-related muscles within the context of age and gender. Twelve rats were equally divided into four groups: (a) 10-month-old females; (b) 10-month-old males; (c) 24-month-old females; and (d) 24-month-old males. Euthanasia of the females was performed in the proestrous phase (vaginal smears) and the masticatory and accessory muscles were removed for immunohistochemical analysis. Statistical analysis was performed with ANOVA and the Tukey test. Estrogen receptor expression was similarly low in all muscles and groups. Testosterone receptor expression in the Masseter muscle of the 24-month-old male rats was higher than that in the other groups and significantly superior to its expression in the Posterior Digastric muscle. In short, testosterone receptor expression was highest in old male rats. If we generalize to humans, this fact could indicate age- and sex-related hormonal influence on temporomandibular muscle dysfunction. Further studies, however, are necessary to strengthen this hypothesis.
Low genotypic diversity and long-term ecological decline in a spatially structured seagrass population
Authors: Alotaibi Nahaa M. , Kenyon Emma J. , Cook Kevan J. , Börger Luca , Bull James C. 
Published: December 2019

AbstractIn isolated or declining populations, viability may be compromised further by loss of genetic diversity. Therefore, it is important to understand the relationship between long-term ecological trajectories and population genetic structure. However, opportunities to combine these types of data are rare, especially in natural systems. Using an existing panel of 15 microsatellites, we estimated allelic diversity in seagrass, Zostera marina, at five sites around the Isles of Scilly Special Area of Conservation, UK, in 2010 and compared this to 23 years of annual ecological monitoring (1996–2018). We found low diversity and long-term declines in abundance in this relatively pristine but isolated location. Inclusion of the snapshot of genotypic, but less-so genetic, diversity improved prediction of abundance trajectories; however, this was spatial scale-dependent. Selection of the appropriate level of genetic organization and spatial scale for monitoring is, therefore, important to identify drivers of eco-evolutionary dynamics. This has implications for the use of population genetic information in conservation, management, and spatial planning.
Changes in hemodynamics associated with metabolic syndrome are more pronounced in women than in men
Authors: Kangas Pauliina , Tikkakoski Antti , Kettunen Jarkko , Eräranta Arttu , Huhtala Heini , Kähönen Mika , Sipilä Kalle , Mustonen Jukka , Pörsti Ilkka 
Published: December 2019

AbstractThe increase in cardiovascular risk associated with metabolic syndrome (MS) seems higher in women than in men. We examined hemodynamics during head-up tilt in 252 men and 250 women without atherosclerosis, diabetes, or antihypertensive medication, mean age 48 years, using whole-body impedance cardiography and radial pulse wave analysis. MS was defined according to Alberti et al. 2009. Men and women with MS presented with corresponding elevations of systolic and diastolic blood pressure (10-14%, p ≤ 0.001) versus controls. Supine pulse wave velocity (16–17%, p < 0.001) and systemic vascular resistance (7–9%, p ≤ 0.026), and upright cardiac output (6–11%, p ≤ 0.008) were higher in both MS groups than controls. Elevation of supine aortic characteristic impedance was higher in women than in men with MS (16% vs. 8%, p = 0.026), and in contrast to men, no upright impedance reduction was observed in women. When upright, women but not men with MS showed faster return of reflected pressure wave (p = 0.036), and smaller decrease in left cardiac work (p = 0.035) versus controls. The faster upright return of reflected pressure, lower upright decrease in left cardiac work, and higher elevation of aortic characteristic impedance may contribute to the greater increase in MS-related cardiovascular risk in women than in men.
Chaotic Dynamics Enhance the Sensitivity of Inner Ear Hair Cells
Authors: Faber Justin , Bozovic Dolores 
Published: December 2019

AbstractHair cells of the auditory and vestibular systems are capable of detecting sounds that induce sub-nanometer vibrations of the hair bundle, below the stochastic noise levels of the surrounding fluid. Furthermore, the auditory system exhibits a highly rapid response time, in the sub-millisecond regime. We propose that chaotic dynamics enhance the sensitivity and temporal resolution of the hair bundle response, and we provide experimental and theoretical evidence for this effect. We use the Kolmogorov entropy to measure the degree of chaos in the system and the transfer entropy to quantify the amount of stimulus information captured by the detector. By varying the viscosity and ionic composition of the surrounding fluid, we are able to experimentally modulate the degree of chaos observed in the hair bundle dynamics in vitro. We consistently find that the hair bundle is most sensitive to a stimulus of small amplitude when it is poised in the weakly chaotic regime. Further, we show that the response time to a force step decreases with increasing levels of chaos. These results agree well with our numerical simulations of a chaotic Hopf oscillator and suggest that chaos may be responsible for the high sensitivity and rapid temporal response of hair cells.
Proteasome inhibition disrupts the metabolism of fumarate hydratase- deficient tumors by downregulating p62 and c-Myc
Authors: Sourbier Carole , Ricketts Christopher J. , Liao Pei-Jyun , Matsumoto Shingo , Wei Darmood , Lang Martin , Railkar Reema , Yang Youfeng , Wei Ming-Hui , Agarwal Piyush , Krishna Murali , Mitchell James B. , Trepel Jane B. , Neckers Len , Linehan W. Marston 
Published: December 2019

AbstractHereditary leiomyomatosis and renal cell carcinoma (HLRCC) is characterized by germline mutations of the FH gene that encodes for the TCA cycle enzyme, fumarate hydratase. HLRCC patients are at risk for the development of an aggressive form of type 2 papillary renal cell carcinoma. By studying the mechanism of action of marizomib, a proteasome inhibitor able to cross the blood-brain barrier, we found that it modulates the metabolism of HLRCC cells. Marizomib decreased glycolysis in vitro and in vivo by downregulating p62 and c-Myc. C-Myc downregulation decreased the expression of lactate dehydrogenase A, the enzyme catalyzing the conversion of pyruvate to lactate. In addition, proteasomal inhibition lowered the expression of the glutaminases GLS and GLS2, which support glutamine metabolism and the maintenance of the redox balance. Thus, in HLRCC cells, proteasome inhibition disrupts glucose and glutamine metabolism, restricting nutrients and lowering the cells’ anti-oxidant response capacity. Although the cytotoxicity induced by proteasome inhibitors is complex, the understanding of their metabolic effects in HLRCC may lead to the development of effective therapeutic strategies or to the development of markers of efficacy.
Synthesis and Characterization of Zirconium Nitride Nanopowders by Internal Gelation and Carbothermic Nitridation
Authors: Zhao Shijiao , Ma Jingtao , Xu Rui , Lin Xuping , Cheng Xing , Hao Shaochang , Zhao Xingyu , Deng Changsheng , Liu Bing 
Published: December 2019

AbstractZirconium compounds has been widely attention over the last decades due to its excellent physical and chemical properties. Zirconium nitride nanopowders were synthesized via a simple direct carbothermic nitridation process of internal gel derived zirconia in the presence of nano-sized carbon black. The effects of reaction temperature, dwell time and molar ratio of carbon black to Zr (C/Zr) on the phase composition, grain size and crystal parameters of products were studied. Based upon the analysis of crystallite phase evolution and microstructure characterization, it was found that zirconium oxynitride is intermediate product and then O atoms in oxynitride were extracted by oxygen getter, carbon black. Anion sites were directly replaced by N atoms to form rock-salt type nitride in carbothermic nitridation process.
Tailored modern GERD therapy – steps towards the development of an aid to guide personalized anti-reflux surgery
Authors: Nikolic Milena , Schwameis Katrin , Paireder Matthias , Kristo Ivan , Semmler Georg , Semmler Lorenz , Steindl Ariane , Mosleh Berta O. , Schoppmann Sebastian F. 
Published: December 2019

AbstractAs the incidence of gastroesophageal reflux disease (GERD) is rising, surgical treatment is continuously advancing in an effort to minimize side effects, whilst maintaining efficacy. From a database of patients that underwent anti-reflux surgery at our institution between 2015 and 2018, the last 25 consecutive patients that underwent electrical stimulation (ES), magnetic sphincter augmentation (MSA) and Nissen fundoplication (NF), following a personalized treatment decision aid, were included in a comparative analysis. After preoperative evaluation each patient was referred for an ES, MSA or NF based on esophageal motility, hiatal hernia (HH) size and the patients’ preferences. Postoperative gastrointestinal symptoms and GERD-Health-related-Quality-of-Life were assessed. Preoperatively the median DCI (299 ES vs. 1523.5 MSA vs. 1132 NF, p = 0.001), HH size (0.5 cm ES vs. 1 cm MSA vs. 2 cm NF, p = 0.001) and presence of GERD-related symptoms differed significantly between the groups. The highest rate of postoperative dysphagia was seen after MSA (24%, p = 0.04), while the median GERD HRQL total score was equally distributed between the groups. The positive short-term postoperative outcome and patient satisfaction indicate that such an aid in treatment indication, based on esophageal motility, HH size and patient preference, represents a feasible tool for an ideal choice of operation and an individualized therapy approach.
Genome-wide human brain eQTLs: In-depth analysis and insights using the UKBEC dataset
Authors: Sng Letitia M. F. , Thomson Peter C. , Trabzuni Daniah 
Published: December 2019

AbstractUnderstanding the complexity of the human brain transcriptome architecture is one of the most important human genetics study areas. Previous studies have applied expression quantitative trait loci (eQTL) analysis at the genome-wide level of the brain to understand the underlying mechanisms relating to neurodegenerative diseases, primarily at the transcript level. To increase the resolution of our understanding, the current study investigates multi/single-region, transcript/exon-level andcisversustrans-acting eQTL, across 10 regions of the human brain. Some of the key findings of this study are: (i) only a relatively small proportion of eQTLs will be detected, where the sensitivity is under 5%; (ii) when an eQTL is acting in multiple regions (MR-eQTL), it tends to have very similar effects on gene expression in each of these regions, as well as beingcis-acting; (iii)trans-acting eQTLs tend to have larger effects on expression compared tocis-acting eQTLs and tend to be specific to a single region (SR-eQTL) of the brain; (iv) the cerebellum has a very large number of eQTLs that function exclusively in this region, compared with other regions of the brain; (v) importantly, an interactive visualisation tool (Shiny app) was developed to visualise the MR/SR-eQTL at transcript and exon levels.
Zika virus infection in pregnancy and infant growth, body composition in the first three months of life: a cohort study
Authors: Soares Fernanda , Abranches Andrea D. , Villela Letícia , Lara Sarah , Araújo Daniele , Nehab Sylvia , Silva Leila , Amaral Yasmin , Junior Saint Clair G. , Pone Sheila , Lobkowicz Ludmila , Clemente Nuria Sanchez , Brasil Patricia , Nielsen-Saines Karin , Pone Marcos , Brickley Elizabeth , Moreira Maria Elisabeth 
Published: December 2019

AbstractThe implications of Zika Virus exposure in pregnancy for early infant growth remains poorly described. The main goal of this study is to compare the growth, body composition, and feeding modality of infants in the first three months of life by prenatal Zika Virus exposure status. We selected an analytical cohort of 115 infants born without microcephaly, comprising 56 infants with qRT-PCR confirmed exposure to ZIKV during gestation and 59 infants born to women with presumptively no evidence of ZIKV in pregnancy. Infants were evaluated at birth, 1 and 3 months of age in terms of anthropometrics, body composition All the results were adjusted by maternal age, maternal BMI and gestational age. We observe no differences between anthropometric measurements at birth. Mothers in exposed group showed higher BMI. At 1 month and 3 months of age there were differences in mid arm circumference, arm muscle circumference and fat free mass. Weight and length was less in the ZIKV exposed in pregnancy infants and statistically different at 3 month of age. The findings of this investigation provide new evidence that ZIKV exposure in pregnancy may be associated with differences in body composition.
Perioperative multidisciplinary implementation enhancing recovery after hip arthroplasty in geriatrics with preoperative chronic hypoxaemia
Authors: He Liang , Zhang Ruqiang , Yin Jianwei , Zhang Han , Bu Wuming , Wang Fang , Zhang Furong 
Published: December 2019

AbstractWe investigated risk factors for postoperative serious adverse events (SAEs) in elderly patients with preoperative chronic hypoxaemia undergone total hip arthroplasty (THA) or hemiarthroplasty and performed an implementation to modify and improve clinical outcome. A retrospective medical record review was performed to identify geriatric patients who receiving THA or hemiarthroplasty at a single university teaching hospital, Kunming, Yunnan, China between January 2009 and August 2017. Total of 450 elderly patients were included in the study. Data were collected on baseline characteristics, detailed treatments, and adverse events. Univariate and multivariate logistic regression analysis were used to identify risk factors for SAEs. In multivariate regression analysis, a higher occurrence of general anaesthesia and multiple episodes of hypotension were associated with higher risk of SAEs (general anesthesia: odds ratio [OR] 5.09, 95% confidence interval [CI] 1.96–13.24, P = 0.001; hypotension time: OR 4.29, 95% CI 1.66–11.10, P = 0.003). After the multidisciplinary implementation, the postoperative length of stay was decreased from 15 days to 10 days (P < 0.0001); incidence of SAEs was decreased from 21.1% to 7.0% (P = 0.002), and the all-cause mortality rate within 30 days decreased from 4.6% to 1.0% (P = 0.040). Our observational study demonstrated that an increasing application of general anaesthesia and longer time of hypotension were associated with an increased risk of postoperative SAEs in patients after THA or hemiarthroplasty. Additionally, optimizing stable haemodynamics under higher application of combined-spinal epidural anaesthesia was associated with improved outcome up to 30 days after THA or hemiarthroplasty.
The C-terminal domain of EFA6A interacts directly with F-actin and assembles F-actin bundles
Authors: Macia Eric , Partisani Mariagrazia , Wang Hong , Lacas-Gervais Sandra , Le Clainche Christophe , Luton Frederic , Franco Michel 
Published: December 2019

AbstractThe Arf6-specific exchange factor EFA6 is involved in the endocytic/recycling pathway for different cargos. In addition EFA6 acts as a powerful actin cytoskeleton organizer, a function required for its role in the establishment of the epithelial cell polarity and in neuronal morphogenesis. We previously showed that the C-terminus of EFA6 (EFA6-Ct) is the main domain which contributes to actin reorganization. Here, by in vitro and in vivo experiments, we sought to decipher, at the molecular level, how EFA6 controls the dynamic and structuring of actin filaments. We showed that EFA6-Ct interferes with actin polymerization by interacting with and capping actin filament barbed ends. Further, in the presence of actin mono-filaments, the addition of EFA6-Ct triggered the formation of actin bundles. In cells, when the EFA6-Ct was directed to the plasma membrane, as is the case for the full-length protein, its expression induced the formation of membrane protrusions enriched in actin cables. Collectively our data explain, at least in part, how EFA6 plays an essential role in actin organization by interacting with and bundling F-actin.