bluuebunny/crossref_metadata_2025_split huggingface dataset:
The decrease of intraflagellar transport impairs sensory perception and metabolism in ageing
Authors: Zhang Yincong , Zhang Xiaona , Dai Yumin , Song Mengjiao , Zhou Yifei , Zhou Jun , Yan Xiumin , Shen Yidong 
Published: July 2020

Abstract
Sensory perception and metabolic homeostasis deteriorate with ageing, impairing the health of aged animals. However, the mechanisms underlying their deterioration remain poorly understood. The potential interplay between the declining sensory perception and the impaired metabolism during ageing is also barely explored. Here, we report that the intraflagellar transport (IFT) in the cilia of sensory neurons is impaired in the aged nematode Caenorhabditis elegans due to a daf-19/RFX-modulated decrease of IFT components. The impaired IFT in sensory cilia in turn causes the deterioration of sensory perception with ageing. Moreover, whereas the IFT-dependent decrease of sensory perception in aged worms has a mild impact on the insulin/IGF-1 signalling, it remarkably suppresses AMP-activated protein kinase (AMPK) signalling across tissues. Upregulating daf-19/RFX effectively enhances IFT, sensory perception, AMPK activity and autophagy, promoting metabolic homeostasis and longevity. Our studies have uncovered an ageing pathway causing IFT decay and sensory perception deterioration, which in turn disrupts metabolism and healthy ageing.
Transdiaphragmatic Single-Port Video-Assisted Thoracoscopic Surgery; a Novel Approach for Pulmonary Metastasectomy through Laparotomy Incision – Case Series
Authors: Jalil Riad Abdel , Chaar Mohamad K. Abou , Shihadeh Omar M. , Al-Qudah Obada , Gharaibeh Azza , Dimashki Loulia Al , Dabous Ali , Ghanem Rami , Al-Edwan Ahed 
Published: July 2020

Abstract
Background: Pulmonary metastasectomy was performed in the early twentieth century and ever since, it has evolved to be one of the main treatment options for certain metastatic malignancies. The advancement of minimally invasive procedures enabled new techniques to minimize morbidity and improve patient quality of care and overall outcome. Cases presentation: Herein we present three patients, aged 53, 48, and 27 years, known to have sigmoid, rectal, and non-seminomatous germ cell tumors respectively. All patients were diagnosed to have metastatic lung nodules and underwent laparotomy to excise abdominal tumors followed by trans-diaphragmatic single-port video-assisted thoracoscopic pulmonary metastasectomy. All patients achieved complete surgical tumor excision, and none had pulmonary related complications on follow-up.Conclusion: Our prescribed novel trans-diaphragmatic single-port video-assisted thoracoscopic surgery (VATS) technique for synchronous pulmonary metastasectomy and intra-abdominal tumor resection is safe and can achieve complete resection with negative margins.
Gene Risk Signature of m6A Regulators, KIAA1429, YTHDF1, YTHDF2, METTL3 and Its Relationship with Immune Infiltration in Hepatocellular Carcinoma
Authors: Guo Jingjing , Wang Liwei , Xu Hanfeng , Zhu Chuandong , Wang Jianning , Zheng Qin 
Published: July 2020

Abstract
Background: Hepatocellular carcinoma (HCC) is a tumor of the digestive system with high mortality. N6-methyladenosine (m6A) is one of the most common forms of mRNA modification. Tumor neoantigens play an important role in anti-tumor immune response and predict the clinical response of immunotherapy. There are few studies on the relationship between m6A regulators and immune infiltrating cells. The objective of this study was to determine the gene expression and prognostic value of m6A regulators in hepatocellular carcinoma. Further, we explored the relationship between m6A regulators and immune-infiltrating cells.Methods: 13 m6A regulators expressions in 374 cancer tissues and 50 normal tissues were analyzed using RNA-seq data and clinical information in the TCGA database. Survival package was used to analyze the survival of patients in the two groups, and the corresponding clinical characteristics and gene expression were analyzed using univariate and multivariate Cox regression. We evaluated the expression of KIAA1429, YTHDF1, YTHDF2, and METTL3 in HCC and its correlation with TIICs using TIMER and CIBERSORT.Results: Most m6A regulators had higher expression levels in 374 cancer tissues. We confirmed two groups of HCC patients using a consensus clustering method. The prognosis of the cluster 1 group was poor compared with that of the cluster 2 group. The m6A regulators, KIAA1429, YTHDF1, YTHDF2, and METTL3 are highly expressed in the high-risk group of HCC. Furthermore, it was found that four m6A regulators (KIAA1429, YTHDF1, YTHDF2, and METTL3) are closely related to the clinicopathological characteristics and poor prognostic marker of hepatocellular carcinoma. We analyzed the correlation between KIAA1429, YTHDF1, YTHDF2, and METTL3 expression and the level of immune invasion of HCC.Conclusion: KIAA1429, YTHDF1, YTHDF2, and METTL3 as m6A regulators may regulate the tumor microenvironment through tumor immune infiltration cells to exert immune anti-tumor effects. KIAA1429, YTHDF1, YTHDF2, and METTL3 as molecular markers providing a new target for therapy of HCC in the further.
Hassle Faced with Induced Mania in Elderly Female While Treating for OC Symptoms. A Case Report.
Authors: BHARTI POONAM , singh Angad harshbir , Gupta Parul 
Published: July 2020

Abstract
Background- Obsessive-compulsive disorder (OCD) is one of the most frequently associated comorbidities in bipolar disorder (BD). While this presents a challenge in understanding the phenomenology and also the treatment aspect of co occurrence of mania with OCD. Case history- The index case is of an elderly female who presented with OC symptoms and while on treatment had mania episodes. The mania episodes presented challenges while managing underlying OC symptoms. Conclusion- The common neurobiological mechanism for the co-morbid illness and treatment lacunae are discussed. The serotonin, dopamine, and glutamate having important role in BD-OCD were evaluated to understand the neurobiological basis of BD-OCD. The index case presented with the challenge of understanding the phenomenology of the illness but also presented with opportunity to learn and successfully manage patients with such co-morbidity. Divalproex and risperidone combo was found to be effective in controlling mania in OCD patients.
An Affordance-based Approach for The Design of Customized Product Non-standard Part
Authors: Lemiao QIU , Heng LI , Guodong YI , Xiaojian LIU , Peng ZHANG , Shuyou ZHANG , Jianrong TAN 
Published: July 2020

Abstract
In this paper we develop an affordance-integrated approach to support design of the customized product non-standard part from conceptual period to detailed period. Firstly, the part affordances are identified by geometrical correlation analysis and shown by liaison graph. Secondly, a force transmission based method is developed to identify the main design parameters of a part, at the same time the design parameters and user requirements both can be mapped into the affordances. In turn, these affordances can be used as a bridge to construct the mapping relationship between the user requirements and the design parameters. In the end, both the force transmission method and the mapping relations are used to construct the detailed model of the non-standard part. A rear platen from the injection molding machine is employed to demonstrate the proposed approach and the result shows that this method can be feasible and useful in product design.
Community-Based Survey On Reported Increased Mortality In Kano State
Authors: Gambo Yusuf Yau 
Published: July 2020

Abstract
Objectives: The main objectives of the study were to use data to corroborate the reported mysterious deaths being recorded in Kano state of Nigeria and find possible explanations to the causes. Study design: This was a cross-sectional study. Methods: With total lockdown in force at the time of conducting the survey, it was not possible to get data through one-on-one interview. Instead, an online survey form was developed and shared widely among residents in the state. The form captured quantitative data about symptoms and circumstances for the deceased and the respondents were only the state residents that witnessed the death cases in their respective communities. Results: A total of 260 responses from various local government areas (LGAs) were received within a period of four days beginning from April 21, 2020. About half of the respondents affirmed that the death toll in their communities within the last two weeks before the survey were in multiples and most of the deaths started to occur from April 13 2020. Moreover, since then, the rate of deaths reported did not decline during the period of the research. Conclusion: There was a sudden increase in the number of deaths due to acute illness suggestive of a disease. Highest number of deaths was recorded among senior adults, affecting males than females. The most affected area was Kano Municipal which was one of the populous LGA in the state. Although it was hard to point out the possible cause of death based on the data, however, the possibility of linking the situation to the current COVID-19 pandemic could not be ruled out. This was because the data strongly aligned with the pandemic in terms of symptoms, incubation period, age group affected and other demographic characteristics.
MiR-125b-5p Inhibited The Progression of Hepatoblastoma As A Shared miRNA of The lncRNA NEAT1 and YES1
Authors: Jin Zhu , Chen Yutong , Mao Yuchen , Gao Mingjuan , Zheng Zebing , Tang Chengyan , Huang Lu , Qu Yan , Liu Yuanmei 
Published: July 2020

Abstract
Background: microRNAs have been studied widely in hepatoblastoma. However, the role of miR-125b-5p and its relationship with the lncRNA sNEAT1 and YES1 in hepatoblastoma have not been reported previously. We aimed to reveal the role of NEAT1/miR-125b-5p/YES1 in the progression of hepatoblastoma.Methods: We collected tumor tissues and their adjacent tissues from 12 hepatoblastoma patients. qRT-PCR was applied to detect the expression of miR-125b-5p, and the relationship of miR-125b-5p with clinicopathological characteristics was analyzed. Dual luciferase reporter assays and RNA pull down assays were used to identify the relationships among NEAT1, miR-125b-5p and YES1. CCK8, Transwell assays and wound healing assays were used to examine cell viability, invasion and migration. In vivo experiments were also applied to detect the effect of miR-125b-5p on hepatoblastoma.Results: miR-125b-5p was significantly downregulated in hepatoblastoma tissue and cells. The higher the PRETEXT grade, the lower the miR-125b-5p level. NEAT1 could bind to miR-125b-5p and inhibit its expression. miR-125b-5p could target YES1 and inhibit its expression. Overexpression of miR-125b-5p decreased the proliferation, invasion, and migratory ability of hepatoblastoma cells. YES1 could rescue the above effects. At the same time, overexpression of miR-125b-5p resulted in decreased YES1 and tumor growth inhibition in vivo.Conclusion: miR-125b-5p acted as a shared miRNA of NEAT1 and YES1 in hepatoblastoma. Overexpression of miR-125b-5p could target YES1 and inhibit its expression, therefore inhibiting the progression of hepatoblastoma.
Prognostic Significance of COVID-19 Receptor ACE2 and Recommendation For Anti-Hypertensive Drug in Renal Cell Carcinoma
Authors: Kim Kihun , Ko Yeji , Ko Dai Sik , Kim Yun Hak 
Published: July 2020

Abstract
Background: Owing to its worldwide spread, the coronavirus disease (COVID-19) epidemic was declared a pandemic by the World Health Organization on March 11, 2020. Angiotensin-converting enzyme 2 (ACE2) is the outer surface protein of the cell membrane that is abundantly distributed in the heart, lungs, and kidneys, and plays an important role in molecular docking of the severe acute respiratory syndrome coronavirus 2. In this study, we aimed to analyze the difference in the survival rate according to ACE2 expressions in pan-cancer. Methods: The clinical and genomic data of pan-cancer patients were accessed from The cancer Genome Atlas. To identify the prognostic significance of ACE2, we used Kaplan-Meier with log-rank test, and the Cox proportional hazards regression to analyze prognostic significance. Results: In the Kaplan-Meier curve, clear cell renal cell carcinoma (ccRCC), uveal melanoma, and prostate adenocarcinoma showed statistically significant. In the Cox regression, thyroid carcinoma and glioblastoma multiforme, and ccRCC showed significant results. Only ccRCC had statistically significant, and high ACE2 expression is related to good prognosis. Conclusions: It is known that ACE inhibitor, a primary antihypertensive agent, increases ACE2 expression. Based on these results, we believe that the ACE inhibitor will be important to increase the lifespan of ccRCC patients. This study is the first research to offer a recommendation on the use of anti-hypertensive drugs to ccRCC patients.
Biological characteristics of rt181T/sW172* HBV are similar among distinct genotypes
Authors: Huaqiang Wang , Jun Ai , Jiajuan Zhang , Wenkai Zheng , Maorong Wang , Li Ping 
Published: July 2020

Abstract
Background: The influence of genotypes on disease progression and clinical outcome of HBV infection is noted. The impact of HBV genotypes on rtA181T/sW172* mutation remains unclear. Patients and Methods: Clinical characteristics of 69 patients with rtA181T/sW172* mutation and 39 patients with rtA181V mutation were analyzed in this study. Fifteen serum specimens with rtA181T/sW172* mutation from different genotypes were collected for cloning and sequence analysis. HBV markers in HepG2 cells encoding different proportions of rtA181T/sW172* mutation and wild type strains were detected in genotype A, B, C and D, respectively.Results: No statistically difference was detected between rtA181T and rtA181V group regarding mean age, sex ratio, liver functions, HBV DNA load and HBeAg positive rate, except for HBsAg level (rtA181T group = 3.07±0.54 lg IU/ml vs rtA181V group = 3.29±0.43 lg IU/ml, P=0.037). Among 69 patients with rtA181T/sW172* mutation, HBV genotypes B and C accounted for 17.0% and 83.0%, respectively. The rate of genotype B was lower in rtA181T group (10.1%) than that in rtA181V group (29.7%), whereas genotype C was higher in rtA181T group (89.9%) than that in rtA181V group (70.3%). HBV rtA181T/sW172* mutant coexisted with wild type strains, accounted for 25% to 90% in all HBV strains. The distribution proportion showed no statistical difference between genotype B and C (59.2%±24.3% vs. 61.2%±19.1% , P=0.86). In transfection experiments, the level of HBV DNA was the highest for genotype B, while HBsAg was expressed in the highest level for genotype A. HBsAg and virus particle were barely detected in the supernatants of rtA181T/sW172* HBV clones in all genotypes. As the proportion of wild type HBV plasmid increased, deficiency of rt181T/sW172* mutation was complemented in all genotypes. No significant difference of the relative expression was found among distinct genotypes (P >0.05).Conclusions: HBV rtA181T/sW172* mutational pattern may be influenced by genotypes, but biological characteristics of this mutation is similar among distinct genotypes.
Boosting effects of MiR-4262 on acute myeloid leukemia advancement via governing KLF6
Authors: Qu Hong , Chen Yongfang , Zeng Wenjing , Huang Xiaohua , Cheng Shuqin 
Published: July 2020

Abstract
Background: Purpose of this study was to explore the influence of miR-4262 on the progression of acute myeloid leukemia (AML) and its molecular mechanism.Methods: Quantitative real-time polymerase chain reaction (qRT-PCR) was carried out to assess the expression of miR-4262 in AML serum and cell lines. MTT, Transwell assays and flow cytometry were adopted to investigate the effect of miR-4262 on cell proliferation, invasion, migration and apoptosis abilities of HL-60 cells respectively. Luciferase reporter assay was conducted to reveal the target relationship of miR-4262 and KLF6. Western blot analysis was utilized to evaluate the expression level of proteins.Results: Relative expression of miR-4262 was up-regulated in AML serum and cell lines (P<0.05). miR-4262 expression was closely related to FAB classification (P=0.002) of AML patients. miR-4262 mimics could promotes the proliferation, invasion and migration of HL-60 cells, while miR-4262 inhibitor is obviously weakened these biological behaviors. Luciferase assay illustrated that miR-4262 can directly interact with KLF6 3’UTR. Up-regulation of miR-4262 could decrease KLF6 level, and increase EGFR level, while the down-regulation of miR-4262 showed the opposite results. Moreover, KLF6 gene knockdown reversed the results caused by miR-4262 inhibitor (P<0.05). Inhibition of KLF6 could significantly promoted the proliferation, invasion and migration of HL-60 cells which is caused by miR-4262 inhibitor.Conclusions: miR-4262 was obviously increased in AML serum and cells, it promotes the progression of AML by regulating KLF6.
Homozygous RRM2 Variant Might Lead to Early Embryo Developmental Arrest: A Case Report
Authors: Wang Xiong , Liu Zhen-teng , Sha Yan-wei , Liu Xiao-yan , Wang Hai-long , Hao Cui-fang , Wu Jian-feng , Wang Jiahui 
Published: July 2020

Abstract
Background: Early embryonic developmental stagnation is one of the reasons that affect the outcome of in vitro fertilization-embryo transfer, leading to the depletion of available embryos or failure after transplantation. It has been shown that defects in the ribonucleotide-reductase lead to cell cycle arrest, developmental delay, and high mutation rates.Case presentation: Two female patients, who were siblings from an inbreeding family, suffered primary infertility of unknown causes and early embryonic developmental arrest during IVF treatment. A total of 39 oocytes were obtained from the patients in collectively 5 IVF/ICSI cycles, of which 37 were mature eggs, only 2 transplantable embryos were formed, and no pregnancy was achieved. Whole genome sequencing and Sanger sequencing were adopted to identify and confirm variations that might cause early embryo developmental stagnation in this family. We identified a homozygous variant c.262C>T:p.His88Tyr in ribonucleotide-diphosphate reductase subunit M2 (RRM2) in both patients and their parents each carried a heterozygous allele. Pedigree analysis showed an autosomal recessive inheritance pattern. Function of this variant was predicted by online databases, which indicated it to be a potential pathogenic mutation. Conclusions: We identified RRM2 as a potential causative gene for early embryonic developmental stagnation. It was also suggested that RRM2 might be a maternal effect gene.
The incidence, risk factors and prognosis of acute kidney injury in severe and critically ill patients with COVID-19 in mainland China: a retrospective study
Authors: Sang Ling , Chen Sibei , Zheng Xia , Guan Weijie , Zhang Zhihui , Liang Wenhua , Zhong Ming , Jiang Li , Pan Chun , Zhang Wei , Xia Jiaan , Chen Nanshan , Wu Wenjuan , Wu Hongkai , Xu Yonghao , Liu Xuesong , Liu Xiaoqing , He Jianxing , Li Shiyue , Zhang Dingyu , Zhong Nanshan , Li Yimin 
Published: July 2020

Abstract
Background: Since the clinical correlates, prognosis and determinants of AKI in patients with Covid-19 remain largely unclear, we perform a retrospective study to evaluate the incidence, risk factors and prognosis of AKI in severe and critically ill patients with Covid-19.Methods: We reviewed medical records of all adult patients (>18 years) with laboratory-confirmed Covid-19 who were admitted to the intensive care unit (ICU) between January 23rd 2020 and April 6th 2020 at Wuhan JinYinTan Hospital and The First Affiliated Hospital of Guangzhou Medical University. The clinical data, including patient demographics, clinical symptoms and signs, laboratory findings, treatment [including respiratory supports, use of medications and continuous renal replacement therapy (CRRT)] and clinical outcomes, were extracted from the electronic records, and we access the incidence of AKI and the use of CRRT, risk factors for AKI, the outcomes of renal diseases, and the impact of AKI on the clinical outcomes.Results: Among 210 subjects, 131 were males (62.4%). The median age was 64 years (IQR: 56-71). Of 92 (43.8%) patients who developed AKI during hospitalization, 13 (14.1%), 15 (16.3%) and 64 (69.6%) patients were classified as stage 1, 2 and 3, respectively. 54 cases (58.7%) received CRRT. Age, sepsis, Nephrotoxic drug, IMV and elevated baseline Scr were associated with AKI occurrence. The renal recover during hospitalization among 16 AKI patients (17.4%), who had a significantly shorter time from admission to AKI diagnosis, lower incidence of right heart failure and higher P/F ratio. Of 210 patients, 93 patients deceased within 28 days of ICU admission. AKI stage 3, critical disease, greater age and minimum P/F <150mmHg independently associated with it.Conclusions: Among patients with Covid-19, the incidence of AKI was high. age , sepsis, nephrotoxic drug, IMV and baseline Scr were strongly associated with the development of AKI. Time from admission to AKI diagnosis, right heart failure and P/F ratio were independently associated with the potential of renal recovery. Finally, AKI KIDGO stage 3 independently predicted the risk of death within 28 days of ICU admission.
Structure of the HLA-DR-DQ-genotype in children with coeliac disease
Authors: Dmitrieva Yu. A. , Roslavtseva E. A. , Kuryaninova V. A. , Zakharova I. N. , Borovik T. E. , Kasatkina E. N. , Klimov L. Ya. , Ivanova A. V. , Radchenko E. R. , Shuleshko O. V. 
Published: July 2020

Introduction. Coeliac disease is a genetically determined disease associated with the HLA-DQ2 and DQ8 genes. According to European data, these alleles are detected in more than 95% of patients. The results of earlier studies in various regions of the Russian Federation and the constituent republics have shown a lower incidence of HLA-DQ2/DQ8 in children with coeliac disease (60–80%). These data served as a basis for discussion about regional genetic features and the potential for disease development in a significant proportion of patients without DQ2/DQ8 alleles.Methods. Genetic screening was conducted on 275 children with coeliac disease. According to the results of the study, the patients were assigned to genetic risk groups.Results. HLA-DQ2/DQ8-alleles were detected in 274 children (99.6%), with 86.9% of patients having DQ2 molecule in genotype and 12.7% having DQ8 molecule. Among 239 DQ2-positive patients, 33.5% were DQ2 homozygous. The remaining DQ2-positive patients had the following HLA-DR-DQ allele distribution: DR3-DQ2/DR5-DQ7 – 8.7%, DR7-DQ2/DR5-DQ7 – 13.4%, DR3-DQ2/ DRx-DQx – 22.2%, DR7-DQ2/DRx-DQx – 2.2%. In five children the incomplete DQ2 molecule was determined, represented by DQA1*0501 allele, while in four patients DQA1*501 was associated with DQB1*0301 allele forming DR5-DQ7 molecule. We found high and significant risk alleles in 29.1% and 22.2% of cases, while moderate and low risk alleles were found in 27.3% and 21.4% of cases respectively.Conclusions: In Russian children with coeliac disease the prevalence of HLA-DQ2/DQ8 alleles corresponds to European data. Low detection frequency of HLA-DQ2/DQ8 molecules in Russian coeliac patients, which was reported earlier, may be due to peculiarities of the approach to the disease diagnostics, which do not correspond to modern Russian and international recommendations.