bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of IL-1β-induced ADAMTS-4/-5 and TAK1 on the degradation of cartilage aggrecan in the progression of human osteoarthritis
Authors: Zhang Qiqi , Xu Shenglin , Fang Yinglei , Hua Changcheng , Wang Xiaohe , Hu Yong 
Published: May 2020

Abstract
Objective

Interleukin-1β (IL-1β) is a potentially important cytokine involved in several pathological processes of osteoarthritis (OA). It is well known that a disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS-4/-5) and transforming growth factor-β activated kinase 1 (TAK1) contribute to the degradation of human OA cartilage aggrecan. The purpose of this study was to investigate the mechanism by which IL-1β induced the expression of ADAMTS-4/-5 and TAK1 in human OA cartilage aggrecan degradation.
Methods

The pathological changes of cartilage tissues and chondrocytes were observed by histomorphological analysis. OA chondrocytes were isolated from human articular cartilage tissues and stimulated with 10 ng/ml of IL-1β at the per-designed times (24 h, 48 h, 72 h). Expression of ADAMTS-4/-5 and TAK1 in cartilage tissues and chondrocytes were measured using immunohistochemistry (IHC) and Western blot (WB).
Results

The level of IL-1β in synovial fluid in the normal group was significantly lower than that in OA group. The expression of ADAMTS-4/-5 and TAK1 were simultaneously upregulated in human OA cartilage tissues,and ADAMTS-4 was more positively correlated with TAK1 than ADAMTS-5. Furthermore, the expression of ADAMTS-4/-5 and TAK1 were increased by stimulation with IL-1β in human OA chondrocytes, but no positive correlation was found.
Conclusion

It is proposed that reducing the expression of ADAMTS-4/-5 in prevention of OA may involve the inhibition of TAK1 activity.
Maintenance Efficacy of Low Dose Imiglucerase for Gaucher Disease
Authors: Qiu Zhengqing , Zhang Baohui , Song Yuhang , Zhang Hongbing , Wu Yuting 
Published: May 2020

Abstract
Background

Imiglucerase is the recommended treatment for Gaucher disease (GD), a hereditary metabolic disease. In high risk adults and all children, the minimum recommended dose for long-term maintenance is 30 U/kg/2 weeks. However, the extremely high cost of this enzyme largely hinders its clinical use. The minimal maintenance dose of imiglucerase has thus been a subject of debate. We aimed to analyze the long-term maintenance outcomes of imiglucerase at dosage < 20 U/kg/2 weeks after standard dose.
Methods

Seventeen patients with GD type 1 or GD type 3 were enrolled for analysis. We evaluated maintenance efficacy of imiglucerase on hemoglobin, platelet, visceral volumes and bone conditions during the 7-year follow-up.
Results

Parameters on hemoglobin, platelet, liver, and spleen volumes of all patients were stabilized or improved. Seven out of 14 patients showed bone mineral density improvement. Three out of 16 patients showed worse bone pain; 6 out of 15 patients showed worse Erlenmeyer flask; 6 out of 15 patients showed worse bone infarction; 1 out of 16 patients showed worse marrow infiltration and 3 out of 15 patients showed worse osteonecrosis.
Conclusions

Imiglucerase less than 20 U/kg/2 weeks is enough to maintain blood and visceral parameters, but is not sufficient to stabilize skeletal conditions.
Is Current Depressive Symptoms Associated with the Perception of Poor Neighborhood Quality in Childhood? A Population-based Study
Authors: Guo Jing , Zhu Yingxue , Fang Liming , Fu Mingqi , Li Min 
Published: May 2020

Abstract
Background Elderly depressive symptoms are an increasing important issue worldwide. Poor neighborhood quality in childhood may increase the risk of depressive symptoms in old age from the perspective of life span theory. The aims of this study were to examine the association between the perception of neighborhood quality during childhood and depressive symptoms in older age.Methods Data was taken from the China Health and Retirement Longitudinal Study (CHARLS), and a total of 7207 individuals aged 60 years or older were included. Robust multivariable linear regression analysis was applied to estimate the association between the perception of childhood community quality and depressive symptoms, and to examine the interaction effects of education and childhood community quality on depressive symptoms.Results This study suggested that individuals who perceived the childhood community as unsafe, deficient in close relationship, unclean demonstrated higher risk in suffering from depression. Furthermore, a significant gender difference has been found. However, no significant interaction effect of education revealed.Conclusion This study proposed that the perception of neighborhood quality during childhood is an important factor associated with depressive symptoms in old age. We urge that older adults’ mental health issues could be examined from a childhood neighborhood quality perspective, and call for further steps to promote neighborhood quality lived by Chinese citizens.
Modulation of Plasma Sphingosine-1-Phosphate Levels via Dietary Salt Intervention in Chinese Adults:an Intervention Trial
Authors: Ma Qiong , Chu Chao , Xue Yan-bo , Hu Jia-wen , Zheng Wen-ling , Yan Yu , Wang Ke-ke , Wang Yang , Yuan Yue , Liao Yue-yuan , Chen Chen , Mu Jianjun 
Published: May 2020

Abstract
Background Sphingosine-1-phosphate (S1P), a pleiotropic bioactive sphingolipid metabolite, is involved in various pathophysiological processes,including blood pressure regulation. Salt is a crucial factor for blood pressure modulation,especially in salt-sensitive individuals who may develop earlier, more severe subclinical target organ damage than salt-resistant individuals.However, the relationships among salt intake, circulating S1P levels, and blood pressure changes are unknown. Thus, we conducted this intervention trial to explore the effect of dietary salt intake on plasma S1P levels and examine the relationship between S1P and blood pressure in Chinese adults.Methods Forty-two participants (aged 18–65 years) were recruited from a rural community in Shaanxi, China. All participants first maintained their normal diet for 3 days, then sequentially ate a low-sodium diet (3.0 g/day NaCl) for 7 days, followed by a high-sodium diet (18.0 g/day NaCl) for 7 days. We assessed their plasma S1P concentrations on the last day of each intervention phase by liquid chromatography–tandem mass spectrometry. We classified the subjects who demonstrated at least a 10% increase in mean arterial pressure upon transitioning from a low-salt to a high-salt diet as salt-sensitive and the others as salt-resistant. Differences in repeated measures were analyzed by repeated-measures analysis of variance.Results Plasma S1P levels decreased significantly from the baseline to low-salt diet period and increased from the low-salt tohigh-salt diet period. We observed this response in both salt-sensitive and salt-resistant individuals. Plasma S1P levels positively correlated with 24-hour urinary sodium excretion, but not 24-hour urinary potassium excretion. In line with plasma S1P level responses to salt intervention, systolic blood pressure and mean arterial pressure decreased from the baseline to low-salt diet period and increased from the low-salt to high-salt period.Systolic blood pressure positively correlated with plasma S1P; the correlation was stronger in salt-sensitive individuals than in salt-resistant individuals.Conclusion Low-salt intervention decreased plasma S1P levels, whereas high-salt intervention reversed this changein Chinese adults. This finding provides evidence that salt moderation may be a high-efficiency, low-cost intervention for regulating circulating S1P levels, with implications for salt-induced blood pressure modulation.Trial registration:NCT02915315.Registered 27 September,2016,http://www.clinicaltrials.gov
Relationship between physical strength measurements and anthropometric measurements: examples from Santal labourer population of Birbhum District, West Bengal, India
Authors: MALAKAR BISWANATH , Roy Subrata K. , Pal Baidyanath 
Published: May 2020

Abstract
Background: Muscular/ physical strength are fundamental in order to satisfy basic needs for survival and productivity, especially among labourer population in developing countries. Research suggests physical body dimension is one of the key determinants of strength; however, the relationship between overall physical body dimension and strength is yet to explored. Aims: to explore the relationship between strength measurements and anthropometric profile of Santal labourer population.Subjects and methods: Cross sectional data on strength (handgrip and back strength) and anthropometric measurements were taken from 536 healthy adult Santal labourers (Male=251, Female=285) aged between 18 to 65 years of Birbhum district, West Bengal. Statistical analysis including Pearson’s correlation and canonical correlation analysis (CCA) were used. Results: Strength negatively correlated with age and positively correlated with most of anthropometric measurements. CCA between strength and anthropometry were 0.765 (p<0.001, explained 85.53% total variance) and 0.611 (p<0.001, explained 71.41% total variance) for males and females respectively. Cross-loading value of CCA indicate fat-free-mass, forearm circumference and weight for male and fat-free mass, chest circumference and wrist breadth for female were most important predictors of strength.Conclusion: Fat-free mass, forearm circumference, weight, chest circumference, wrist breadth were the key measurements for overall strength of Santal labourer population.
Midwives’ expectations from pregnant women regarding their participation in safe delivery: a qualitative study
Authors: Bayrami Roghieh , Baghaei Rahim 
Published: May 2020

Abstract
Background : Pregnant women’s participation in safe delivery is associated with safety assurance and improvement. Safety and care quality improvement in developed countries has so far been based on research and clinical evidence. Nonetheless, stakeholders’ preferences and expectations were not much taken into account. This study aimed to explore midwives’ expectations from pregnant women regarding their participation in safe delivery. Methods : This qualitative study was conducted in 2019 in three public hospitals in Urmia, Iran. Data were collected through 24 in-depth semi-structured interviews with 24 midwives. Sampling was done purposively and continued up to data saturation. Data were analyzed through Graneheim and Lundman’s qualitative content analysis. Results: Midwives’ expectations from pregnant women regarding their participation in safe delivery were grouped into the two main themes of effective communication (with two main categories) and empowerment of pregnant women (with three categories). The five main categories of these two themes were client’s interaction with midwife, confidence in midwife, readiness for delivery, health literacy improvement, and participation in safe behaviors, respectively. Conclusions : Understanding midwives’ expectations regarding pregnant women participation in safe delivery is important to the development of policies for care quality and safety improvement in perinatal care. Keywords : Patient participation, Safe delivery, Qualitative study
Where is the best cup position for Crowe III hips with the presence of a false acetabulum in total hip arthroplasty?
Authors: Shen Junmin , Sun Jingyang , Du Yinqiao , Ma Haiyang , Zhang Bohan , Zhou Yonggang 
Published: May 2020

Abstract
Background

We aimed to evaluate the clinical and radiological results of three different cup positions for Crowe III hips with a false acetabulum.
Methods

From January 2008 to December 2018, we retrospectively evaluated 61 hips (57 patients) which were classified as Crowe type III and presented a false acetabulum. Based on the position of cup on postoperative radiographs, all hips were divided into three groups: anatomic group (A group), false acetabular group (F group) and middle group (M group).
Results

There were no significant differences in comparison of anteversion, inclination and leg length discrepancy among three groups. The cup size of A group was significantly smaller than other two groups. In the A group, autograft was utilized in 5 hips (33.3%) and a S-ROM stem was used more frequently. By the comparison of horizontal distance, the F group showed an overmuch lateralization of rotation center. At the last follow-up, the mean Harris hip scores were significantly improved in three groups. There were 2 patients from the M group with both a moderate limp and positive Trendelenburg sign. During the follow-up period, one hip of the M group performed an acetabular revision by the reason of acetabular fracture.
Conclusions

All three cup positions for Crowe III hips with a false acetabulum showed good clinical outcomes and no significant difference. However, compared with the anatomical position, the mid-position allows for larger acetabular cups and liners. Further, the mid-position allows for more mediazation compared with the false acetabular position.
Long Non-coding RNA PTCSC3 Promotes Apoptosis of Human Papillary Thyroid Carcinomas by Regulating S100A4
Authors: Wu Qiong , Jiang Liang , Wu Jiang , Dong HaiFang , Zhao Yaping 
Published: May 2020

Abstract
Objective: We investigated the effect of papillary thyroid carcinoma susceptibility candidate 3 (PTCSC3) on the apoptosis of human papillary thyroid carcinomas cells by regulating S100A4.Methods: Normal cells Nthy-ori 3-1, cancer cells SW579 and TPC-1 were used to study. Proliferation of human papillary thyroid carcinomas cells was detected by CCK8, clone formation and transwell. Tube formation assay was used to observe the formation of in vitro tubes. Western blot was applied to detect the expressions of S100A4. After S100A4 overexpressed or down expressed, the above experiments were repeated. Tumor volume and weight was measured. Ki67 expression and tumor microvascular density (MVD) were detected by immunohistochemistry. Apoptosis of tissues was detected by TUNEL. Expression of apoptosis-related proteins, VEGF and MMP-9 were detected.Results: PTCSC3 overexpression and S100A4 lowexpression had significantly decreased proliferation, invasion, migration and tube formation of cancer cells. Meanwhil, the expression of Ki67 and MVD in tumor tissues were significantly decreased (p<0.05).Conclusion: Up-regulation of PTCSC3 can inhibit the expression of S10044, and promoted cell apoptosis and tube formation of papillary thyroid carcinomas cells.
Clinical-like cryotherapy in acute arthritis of the knee improves inflammation signs, pain, joint swelling and motor performance in mice
Authors: Castro Paula Aiello Tomé de Souza , Machanocker Dafiner Helen , Perez Raphael Sanches , Cunha Thiago Mattar , Barbosa Germana Medeiros , Cunha Jonathan Emanuel , Oliveira Francisco Fábio Bezerra de , Ramalho Fernando Silva , Russo Thiago Luiz , Cunha Fernando Queiroz , Salvini Tania de Fátima 
Published: May 2020

Abstract
Background

Assess the effect of clinical-like cryotherapy on inflammatory signs (in vivo neutrophil migration, cytokines and joint inflammation), pain, and joint swelling in mice with knee arthritis. Balance and motor coordination were also evaluated.
Methods

Young C57BL/6 mice were randomly divided into 3 groups (n = 10/group): control group: animals were not immunized with antigen-induced arthritis (AIA) and no intervention occurred; AIA group: animals immunized with methylated bovine serum albumin (mBSA) for knee arthritis; AIA + cryotherapy group: animals immunized with mBSA and submitted to cryotherapy. After 21days, the study was divided into 6-hour experimental analysis periods after injection-induced joint inflammation with mBSA (100 ug/joint) and a cryotherapy protocol was applied (ice pack, two 20-minute sessions). Number of synovial fluid neutrophils, cytokine levels, joint histology, pain, joint swelling and motor performance were also analyzed.
Results

Our results showed that the application of cryotherapy in mice with acute knee arthritis reduced inflammatory signs and improved the symptoms of pain and joint swelling, as well as motor coordination and balance in the animals.
Conclusions

Clinical-like cryotherapy used in the acute phase of arthritis reduced inflammatory signs and improved the symptoms of pain, joint swelling, motor coordination and balance in the mice.
A multi-lncRNA signature for suvival prediction in esophageal squamous cell carcinoma (ESCC) patients
Authors: zhang Lan , Li Pan , Xing Chenju , Zhu Di , Zhang Jianying , Wang Weiwei , Jiang Guozhong 
Published: May 2020

Abstract
Background: The aim of this study was to identify prognostic long non-coding RNAs (lncRNAs) and develop a multi-lncRNA signature for suvival prediction in esophageal squamous cell carcinoma (ESCC) patients.Methods: The clinical and gene expression data from 301 ESCC patients were downloaded, including a corhort used as training set from Gene Expression Omnibus database (GSE53624, n=119), another cohort of 98 paired ESCC tumor and normal tissues as test set and an independent validation cohort including 84 ESCC tissues. Survival analyses, Cox regression and Kaplan–Meier analysis were used.Results: we screened a prognostic marker of ESCC from the GSE53624 dataset and named it as the five-lncRNA signature including AC007179.1, MORF4L2-AS1, RP11-488I20.9, RP13-30A9.2, RP4-735C1.6, which could classify patients into high- and low-risk groups with significantly different survival(median survival: 1.75 years vs. 4.01 years, log rank P<0.05). Then test dataset and validation dataset confirmed that the five-lncRNA signature can determine the prognosis of ESCC patients. Predictive independence of the prognostic marker was proved by multivariable Cox regression analyses in the three datasets (P<0.05). In addition, the signature was found to be better than TNM stage in terms of prognosis.Conclusion: The five-lncRNA signature could be a good prognostic biomarker for ESCC patients and has important clinical value.
Repression of BET activity sensitizes oral squamous cell carcinoma to PARP inhibition via inhibiting DNA homologous recombination and immune inhibition
Authors: Liu Xiaojing , Zhang Fenghe , Zhang Xin 
Published: May 2020

Abstract
Background: To investigate the root cause of therapeutic resistance underlying oral squamous cell carcinoma (OSCC) to the PARP inhibitor olaparib and explore the combination with the BET inhibitor JQ1 to augment its treatment effects.Material and Methods: Cell viability were detected in the study. We also evaluated the expression of a series of factors through quantitative real-time PCR, immunofluorescence and western blot. The effect of combining JQ1 and olaparib in xenograft OSCC mouse model was also examined.Results: The sensitivity of Cal27 cells to olaparib was better than Scc25. Functional assays demonstrated that olaparib induced HR repair and upregulated PD-L1 expression, which results in drug resistance of OSCC to olaparib. Variations of these factors in the two cell lines may explain different sensitivity to olaparib. Moreover, the JQ1 BET inhibitor and olaparib synergistically exhibited anti-cancer effects in OSCC in vitro and in vivo and inhibited essential HR repair factors RAD51, BRCA1, and TOPBP1 through the ATR/CHK1 pathway and immune suppression mediated by the PD-L1 pathway.Conclusions: Elevated HR and PD-L1 are involved in resistance mechanisms of OSCC to olaparib, attenuating its anti-tumor effects. Our results suggest that the strong synergistic anti-cancer activity of combining olaparib with BET inhibitor in OSCC may be via suppression of the ATR/CHK1-mediated DNA damage response and PD-L1-related immune escape, indicating this combination strategy as a possible therapy in OSCC.
Thoughts on Higher Medical Education Under Major Public Health Emergencies: Thinking Ahead After COVID-19 Outbreak—A case-control study
Authors: Lin Wei , Chen Yan , Shi Songchang , Liang Jixing , Huang Huibin , Li Liantao , Cai Liangchun , Zong Liyao , Wang Nengying , Wen Junping , Chen Gang 
Published: May 2020

Abstract
Background: The spread of coronavirus disease 2019 (COVID-19) has posed great threat to the global population’s health and nearly every medical school throughout the world suspended classes as a precaution against the virus. The aim of this research is to explore the most suitable teaching and learning pattern in medical school during COVID-19 Outbreak. Methods: This was a case-control study which conducted in a medical school. We applied a new blended teaching model based on 5G network that combined team-based learning (TBL) and online interaction to the students before the outbreak and when universities responded to the COVID-19 outbreak by closing campuses, they shifted to different forms of distance learning. Fifty fourth-year medical students receiving the “5+3” pattern courses regarding internal medicine were enrolled in the study. A five-point Likert scale questionnaire that contains 20 items was used to compare the effect of the two kinds of teaching patterns. It evaluated the indicators of core competencies of students including professionalism, attitude towards learning, knowledge and learning skills, teamwork skills, motivation in learning, adaptability and acceptance of the courses and network environment.Results: According to the descriptive statistical analysis of the first part of the questionnaire (question 1-16), the average score of adaptability and acceptance of the courses is 2.60 less than 3, indicating that students are more adapted to other forms of distance learning during COVID-19 outbreak; the average score of the rest of the questions is greater than 3, indicating that blended teaching model based on 5G network is superior to other forms of distance learning. The number of male students who are inclined to the blended teaching model based on 5G network is 0.13 times as much as that of female students (95%CI:0.028~0.602, p=0.009).Conclusion: The results that medical education based on 5G network that combined team-based learning (TBL) and online interaction is a more suitable option to teach medical students.