bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of a New Carbon Dioxide System for Autonomous Surface Vehicles
Authors: Sabine Christopher , Sutton Adrienne , McCabe Kelly , Lawrence-Slavas Noah , Alin Simone , Feely Richard , Jenkins Richard , Maenner Stacy , Meinig Christian , Thomas Jesse , van Ooijen Erik , Passmore Abe , Tilbrook Bronte 
Published: July 2020

AbstractCurrent carbon measurement strategies leave spatiotemporal gaps that hinder the scientific understanding of the oceanic carbon biogeochemical cycle. Data products and models are subject to bias because they rely on data that inadequately capture mesoscale spatiotemporal (kilometers and days to weeks) changes. High-resolution measurement strategies need to be implemented to adequately evaluate the global ocean carbon cycle. To augment the spatial and temporal coverage of ocean–atmosphere carbon measurements, an Autonomous Surface Vehicle CO2 (ASVCO2) system was developed. From 2011 to 2018, ASVCO2 systems were deployed on seven Wave Glider and Saildrone missions along the U.S. Pacific and Australia’s Tasmanian coastlines and in the tropical Pacific Ocean to evaluate the viability of the sensors and their applicability to carbon cycle research. Here we illustrate that the ASVCO2 systems are capable of long-term oceanic deployment and robust collection of air and seawater pCO2 within ±2 μatm based on comparisons with established shipboard underway systems, with previously described Moored Autonomous pCO2 (MAPCO2) systems, and with companion ASVCO2 systems deployed side by side.
Facilitators and barriers to utilization of medications for opioid use disorder in primary care in South Carolina
Authors: Oros Sarah M , Christon Lillian M , Barth Kelly S , Berini Carole R , Padgett Bennie L , Diaz Vanessa A 
Published: July 2020

Objective Utilization of medications for opioid use disorder (MOUD) has not been widely adopted by primary care providers. This study sought to identify interprofessional barriers and facilitators for use of MOUD (specifically naltrexone and buprenorphine) among current and future primary care providers in a southeastern academic center in South Carolina. Method Faculty, residents, and students within family medicine, internal medicine, and a physician assistant program participated in focus group interviews, and completed a brief survey. Survey data were analyzed quantitatively, and focus group transcripts were analyzed using a deductive qualitative content analysis, based upon the theory of planned behavior. Results Seven groups ( N = 46) completed focus group interviews and surveys. Survey results indicated that general attitudes towards MOUD were positive and did not differ significantly among groups. Subjective norms around prescribing and controllability (i.e., beliefs about whether prescribing was up to them) differed between specialties and between level of training groups. Focus group themes highlighted attitudes about MOUD (e.g., “opens the flood gates” to patients with addiction) and perceived facilitators and barriers of using MOUD in primary care settings. Participants felt that although MOUD in primary care would improve access and reduce stigma for patients, prescribing requires improved provider education and an integrated system of care. Conclusions The results of this study provide an argument for tailoring education to specifically address the barriers primary care prescribers perceive. Results promote the utilization of active, hands-on learning approaches, to ultimately promote uptake of MOUD prescribing in the primary care setting in South Carolina.
The migraine landscape on YouTube: A review of YouTube as a source of information on migraine
Authors: Saffi Hillah , Do Thien Phu , Hansen Jakob Møller , Dodick David W , Ashina Messoud 
Published: July 2020

Introduction YouTube is the most widely used video hosting website in the world; however, the quality and reliability of information is unknown. The aim of this study is to evaluate the content and distribution of the most popular videos on YouTube about migraine. Methods We searched for migraine-related videos on the online video hosting resource YouTube ( http://youtube.com/ ). Two authors screened the titles and video descriptions independently for all videos with a view count of ≥ 10,000 views. For each video we recorded descriptive data, the source/author and the primary purpose/content. Results We identified 351 eligible videos. In total, there was more than 3 days of content viewed more than 163 million times. Only 9% of these videos were authored by healthcare professionals. The majority (44%) of videos focused on complementary and alternative medicine. Discussion YouTube provides a wide array of easily accessible information on migraine, ranging from authoritative sources to potentially questionable content. If used uncritically, this may result in inadequate clinical management. Peer-reviewed information on migraine mechanisms and treatment is needed to provide the best available evidence for the public and patients. Ideally, a professional society or foundation such as the International Headache Society would develop, curate, and distribute content.
Arterial percutaneous angioplasty in hemodialysis access: Endovascular treatment of hand ischemia
Authors: Teixeira Gabriela , Almeida Paulo , Loureiro Luís , Antunes Inês , Rego Duarte , Teixeira Sérgio , Mendes Daniel , Sousa Clemente N , Queirós José , de Matos Norton , Almeida Rui 
Published: July 2020

Background: Hemodialysis access–induced distal ischemia consists of symptomatic extremity malperfusion after vascular access creation. It is usually caused by discordant vascular resistance, with arteriovenous shunting of a high blood volume from arterial into venous system and subsequent hand hypoperfusion. Less often, hemodialysis access–induced distal ischemia is caused by arterial stenosis. In these cases, access frequently has normal/low flow, radial pulse is usually absent and not recoverable with vascular access digital compression, diabetes is often present, and percutaneous transluminal angioplasty can be critical for access and limb salvage. Methods: Retrospective study conducted between June 2011 and February 2018 of patients with vascular access submitted to arterial percutaneous transluminal angioplasty for limb-threatening ischemia. Results: Twenty-nine patients were referred for arterial angiography after hemodialysis access–induced distal ischemia diagnosis and physical examination or ultrasound findings suggestive of arterial disease. In 11 patients, percutaneous transluminal angioplasty was not technically feasible. Among 18 treated patients, 83.3% had diabetes and 60% had skin ulcerations. Target arteries were radial (11), brachial (7), axillar (2), ulnar (2), and subclavian (1). Clinical success, defined as arteriovenous maintenance and wound healing/pain resolution, was observed in 12 patients (66.7%). Concomitant procedures included adjuvant banding ( n = 2) and finger amputation ( n = 1), and one reintervention was performed. No intra- or postoperative complications were reported. Conclusion: Hemodialysis access–induced distal ischemia is a serious complication of hemodialysis vascular access, with multifactorial etiology. Correct and timely diagnosis is crucial for maintaining access and limb salvage. Percutaneous transluminal angioplasty is a minimally invasive procedure that may be effective and long-lasting in carefully selected patients with ischemic complaints.
Efficacy of Next-Generation EGFR-TKIs in Patients With Non-Small Cell Lung Cancer: A Meta-Analysis of Randomized Controlled Trials
Authors: Qi Yi-Tian , Hou Yi , Qi Liang-Chen 
Published: July 2020

Background: The efficacy of next-generation epidermal growth factor receptor-tyrosine kinase inhibitors in patients with advanced non-small cell lung cancer who have failed first-generation epidermal growth factor receptor-tyrosine kinase inhibitors still remains under investigation. Objective: The aim of this meta-analysis was to systematically assess the efficacy and safety profiles of next-generation epidermal growth factor receptor-tyrosine kinase inhibitors in patients with advanced non-small cell lung cancer who failed first-generation epidermal growth factor receptor-tyrosine kinase inhibitors. Methods: We performed a comprehensive search of several electronic databases up to September 2018 to identify clinical trials. The primary end point was overall survival, progression-free survival, disease controlled rate, objective response rate, and adverse events. Epidermal growth factor receptor-tyrosine kinase inhibitor emergent severe adverse events (grade ≥ 3) were analyzed. Odds ratio along with 95% confidence interval were utilized for main outcome analysis. Results: In total, we had 3 randomized controlled trials in this analysis. The group of next-generation epidermal growth factor receptor-tyrosine kinase inhibitors had significantly improved progression-free survival (odds ratio = 0.34, 95% confidence interval = 0.29-0.40, P < .00001), as well as objective response rate (odds ratio = 10.48, 95% confidence interval = 3.87-28.34, P < .00001) and disease controlled rate (odds ratio = 6.03, 95% confidence interval = 4.41-8.25, P < .00001). However, there was no significant difference in overall survival with next-generation epidermal growth factor receptor-tyrosine kinase inhibitors (odds ratio = 1.05, 95% confidence interval = 0.85-1.31, P = .66). Meanwhile, the odds ratio for treatment-emergent severe adverse events (diarrhea, rash/acne, nausea, vomiting, anemia) between patients who received next-generation epidermal growth factor receptor-tyrosine kinase inhibitors and those who received first-generation epidermal growth factor receptor-tyrosine kinase inhibitors did not show safety benefit ( P > .05). Conclusions: Next-generation epidermal growth factor receptor-tyrosine kinase inhibitors were shown to be the better agent to achieve higher response rate and longer progression-free survival in patients with non-small cell lung cancer as the later-line therapy for previously treated patients with first-generation epidermal growth factor receptor-tyrosine kinase inhibitors. Meanwhile, they did not achieve benefit in overall survival and safety compared with the chemotherapy group. Further research is needed to develop a database of all EGFR mutations and their individual impacts on the various treatments.
Assembly of alternative prothrombinase by extracellular histones initiates and disseminates intravascular coagulation
Authors: Abrams Simon T. , Su Dunhao , Sahraoui Yasmina , Lin Ziqi , Cheng Zhenxing , Nesbitt Kate , Alhamdi Yasir , Harrasser Micaela , Du Min , Foley Jonathan H. , Lillicrap David , Wang Guozheng , Toh Cheng-Hock 
Published: July 2020

AbstractThrombin generation is pivotal to both physiological blood clot formation and pathological development of disseminated intravascular coagulation (DIC). In critical illness, extensive cell damage can release histones into the circulation, which can increase thrombin generation and cause DIC, but the molecular mechanism is not clear. Typically, thrombin is generated by the prothrombinase complex, comprising activated factor X (FXa), activated cofactor V (FVa), and phospholipids to cleave prothrombin in the presence of calcium. In this study, we found that in the presence of extracellular histones, an alternative prothrombinase could form without FVa and phospholipids. Histones directly bind to prothrombin fragment 1 (F1) and fragment 2 (F2) specifically to facilitate FXa cleavage of prothrombin to release active thrombin, unlike FVa, which requires phospholipid surfaces to anchor the classical prothrombinase complex. In vivo, histone infusion into mice induced DIC, which was significantly abrogated when prothrombin F1 + F2 were infused prior to histones, to act as decoy. In a cohort of intensive care unit patients with sepsis (n = 144), circulating histone levels were significantly elevated in patients with DIC. These data suggest that histone-induced alternative prothrombinase without phospholipid anchorage may disseminate intravascular coagulation and reveal a new molecular mechanism of thrombin generation and DIC development. In addition, histones significantly reduced the requirement for FXa in the coagulation cascade to enable clot formation in factor VIII (FVIII)– and FIX-deficient plasma, as well as in FVIII-deficient mice. In summary, this study highlights a novel mechanism in coagulation with therapeutic potential in both targeting systemic coagulation activation and correcting coagulation factor deficiency.
Molecular disparity in human leukocyte antigens is associated with outcomes in haploidentical stem cell transplantation
Authors: Zou Jun , Ciurea Stefan O. , Kongtim Piyanuch , Yi Min , Carmazzi Yudith , Rondon Gabriela , Srour Samer , Partlow David , Champlin Richard E. , Cao Kai 
Published: July 2020

Abstract
Haploidentical donors are increasingly used for patients requiring hematopoietic stem cell transplantation (HSCT). Although several factors have been associated with transplant outcomes, the impact of HLA disparity in haploidentical HSCT (haplo-HSCT) remains unclear. We investigated the impact of HLA disparity quantified by mismatched eplets (ME) load of each HLA locus on the clinical outcome of 278 consecutive haploidentical transplants. Here, we demonstrated that the degree of HLA molecular mismatches, at individual HLA loci, may be relevant to clinical outcome in the haplo-HSCT. A significantly better overall survival was associated with higher ME load from HLA-A (hazard ratio [HR], 0.97; 95% confidence interval [CI], 0.95-0.99; P = .003) and class I loci (HR, 0.99; 95% CI, 0.97-0.99; P = .045) in the host-versus-graft direction. The apparent survival advantage of HLA-A ME was primarily attributed to reduced risk in relapse associated with an increase in HLA-A ME load (subdistribution HR, 0.95; 95% CI, 0.92-0.98; P = .004). Furthermore, we have identified an association between the risk of grade 3-4 acute graft-versus-host disease (GVHD) and a higher ME load at HLA-B and class I loci in graft-versus-host (GVH) direction. Additionally, GVH nonpermissive HLA-DPB1 mismatch defined by T-cell epitope grouping was significantly associated with relapse protection (subdistribution HR, 0.19; 95% CI, 0.06-0.59; P = .004) without a concurrent increase in GVHD. These findings indicate that alloreactivity generated by HLA disparity at certain HLA loci is associated with transplant outcomes, and ME analysis of individual HLA loci might assist donor selection and risk stratification in haplo-HSCT.
Dynamic Stiffness Analysis and Experimental Verification of Axial Magnetic Bearing Based on Air Gap Flux Variation in Magnetically Suspended Molecular Pump
Authors: Sun Jinji , Wei Wanting , Tang Jiqiang , Wang Chun-E 
Published: July 2020

AbstractCurrent and displacement stiffness are important parameters of axial magnetic bearing (AMB) and are usually considered as constants for the control system. However, in actual dynamic work situations, time-varying force leads to time-varying currents and air gap with a specific frequency, which makes the stiffness of appear decrease and even worsens control performance for the whole system. In this paper, an AMB dynamic stiffness model considering the flux variation across the air gap due to frequency is established to obtain the accurate dynamic stiffness. The dynamic stiffness characteristics are analyzed by means of the dynamic equivalent magnetic circuit method. The analytical results show that the amplitude of current and displacement stiffness decreases with frequency increasing. Moreover, compared with the stiffness model without considering the variation of flux density across the air gap, the improved dynamic stiffness results are closer to the actual results. Through the dynamic stiffness measurement method of AMB, experiments of AMB in magnetically suspended molecular pump (MSMP) are carried out and the experimental results are consistent with theoretical analysis results. This paper proposes the dynamic stiffness model of axial magnetic bearing considering the variation of flux density across the air gap, which improves the accuracy of the AMB stiffness analysis.
Firing activity of locus coeruleus noradrenergic neurons decreases in necdin-deficient mice, an animal model of Prader–Willi syndrome
Authors: Wu Rui-Ni , Hung Wei-Chen , Chen Ching-Tsuey , Tsai Li-Ping , Lai Wen-Sung , Min Ming-Yuan , Wong Shi-Bing 
Published: July 2020

Abstract
Background
Prader–Willi syndrome (PWS) is a neurodevelopmental disorder characterized by multiple respiratory, cognitive, endocrine, and behavioral symptoms, such as central apnea, intellectual disabilities, exaggerated stress responses, and temper tantrums. The locus coeruleus noradrenergic system (LC-NE) modulates a diverse range of behaviors, including arousal, learning, pain modulation, and stress-induced negative affective states, which are possibly correlated with the pathogenesis of PWS phenotypes. Therefore, we evaluated the LC-NE neuronal activity of necdin-deficient mice, an animal model of PWS.

Methods
Heterozygous necdin-deficient mice (B6.Cg-Ndntm1ky) were bred from wild-type (WT) females to generate WT (+m/+p) and heterozygotes (+m/−p) animals, which were examined of LC-NE neuronal activity, developmental reflexes, and plethysmography.

Results
On slice electrophysiology, LC-NE neurons of Ndntm1ky mice with necdin deficiency showed significantly decreased spontaneous activities and impaired excitability, which was mediated by enhanced A-type voltage-dependent potassium currents. Ndntm1ky mice also exhibited the neonatal phenotypes of PWS, such as hypotonia and blunt respiratory responses to hypercapnia.

Conclusions
LC-NE neuronal firing activity decreased in necdin-deficient mice, suggesting that LC, the primary source of norepinephrine in the central nervous system, is possibly involved in PWS pathogenesis.
Proteomic approaches for characterizing renal cell carcinoma
Authors: Clark David J. , Zhang Hui 
Published: July 2020

AbstractRenal cell carcinoma is among the top 15 most commonly diagnosed cancers worldwide, comprising multiple sub-histologies with distinct genomic, proteomic, and clinicopathological features. Proteomic methodologies enable the detection and quantitation of protein profiles associated with the disease state and have been explored to delineate the dysregulated cellular processes associated with renal cell carcinoma. In this review we highlight the reports that employed proteomic technologies to characterize tissue, blood, and urine samples obtained from renal cell carcinoma patients. We describe the proteomic approaches utilized and relate the results of studies in the larger context of renal cell carcinoma biology. Moreover, we discuss some unmet clinical needs and how emerging proteomic approaches can seek to address them. There has been significant progress to characterize the molecular features of renal cell carcinoma; however, despite the large-scale studies that have characterized the genomic and transcriptomic profiles, curative treatments are still elusive. Proteomics facilitates a direct evaluation of the functional modules that drive pathobiology, and the resulting protein profiles would have applications in diagnostics, patient stratification, and identification of novel therapeutic interventions.
Investigating the impact of preselection on subsequent single-step genomic BLUP evaluation of preselected animals
Authors: Jibrila Ibrahim , ten Napel Jan , Vandenplas Jeremie , Veerkamp Roel F. , Calus Mario P. L. 
Published: July 2020

Abstract
Background
Preselection of candidates, hereafter referred to as preselection, is a common practice in breeding programs. Preselection can cause bias and accuracy loss in subsequent pedigree-based best linear unbiased prediction (PBLUP). However, the impact of preselection on subsequent single-step genomic BLUP (ssGBLUP) is not completely clear yet. Therefore, in this study, we investigated, across different heritabilities, the impact of intensity and type of preselection on subsequent ssGBLUP evaluation of preselected animals.

Methods
We simulated a nucleus of a breeding programme, in which a recent population of 15 generations was produced with PBLUP-based selection. In generation 15 of this recent population, the parents of the next generation were preselected using several preselection scenarios. These scenarios were combinations of three intensities of preselection (no, high or very high preselection) and three types of preselection (genomic, parental average or random), across three heritabilities (0.5, 0.3 or 0.1). Following each preselection scenario, a subsequent evaluation was performed using ssGBLUP by excluding all the information from the preculled animals, and these genetic evaluations were compared in terms of accuracy and bias for the preselected animals, and in terms of realized genetic gain.

Results
Type of preselection affected selection accuracy at both preselection and subsequent evaluation stages. While preselection accuracy decreased, accuracy in the subsequent ssGBLUP evaluation increased, from genomic to parent average to random preselection scenarios. Bias was always negligible. Genetic gain decreased from genomic to parent average to random preselection scenarios. Genetic gain also decreased with increasing intensity of preselection, but only by a maximum of 0.1 additive genetic standard deviation from no to very high genomic preselection scenarios.

Conclusions
Using ssGBLUP in subsequent evaluations prevents preselection bias, irrespective of intensity and type of preselection, and heritability. With GPS, in addition to reducing the phenotyping effort considerably, the use of ssGBLUP in subsequent evaluations realizes only a slightly lower genetic gain than that realized without preselection. This is especially the case for traits that are expensive to measure (e.g. feed intake of individual broiler chickens), and traits for which phenotypes can only be measured at advanced stages of life (e.g. litter size in pigs).
An Endostatin-lentivirus (ES-LV)-EPC gene therapy agent for suppression of neovascularization in oxygen-induced retinopathy rat model
Authors: Ai Jing , Ma Jian , Chen Zhi-Qing , Sun Jun-Hui , Yao Ke 
Published: July 2020

AbstractBackgroundTransplantation of gene transfected endothelial progenitor cells (EPCs) has provided novel methods for tumor neovascularization therapy but not for ocular disease therapy. This study aimed to investigate the efficacy of endostatin transfected EPCs in retinal neovascularization therapy.ResultsQuantitative reverse transcription-polymerase chain reaction (qRT-PCR) showed the high expression of endostatin in endostatin-lentivirus-EPCs. The neovascularization leakage area and the number of preretinal neovascular cell nuclei were significantly decreased in the endostatin-lentivirus and endostatin-lentivirus-EPC groups, and the effects of these two treatments on inhibiting retinal neovascularization were almost the same. These two groups also showed the greater retinal distribution of endostatin. Intravitreal injections of endostatin-lentivirus-EPCs inhibited retinal neovascularization, vascular endothelial growth factor (VEGF) and CD31 expression, and increased endostatin expression in vivo. Endostatin-lentivirus-EPCs targeted and prevented pathologic retinal neovascularization.ConclusionsGene-combined EPCs represent a potential new therapeutic agent for the treatment of neovascular eye diseases.