bluuebunny/crossref_metadata_2025_split huggingface dataset:
Risk of climate-induced damage in historic parchment
Authors: Krzemień Leszek , Czyżewska Agata , Soboń Magdalena , Kozłowski Roman , Bratasz Łukasz 
Published: February 2020

Abstract
Moisture adsorption and related dimensional change were examined in several samples of historic and contemporary parchment. The tensile behaviour was determined for contemporary parchment and two selected historic materials. The moisture-related data for most parchments are close to the contemporary material while aging and past treatments may lower adsorption of moisture and, in consequence, the dimensional change induced by changes in the moisture content. Contemporary parchment exhibited larger water vapour adsorption and moisture-related response compared to most historic materials and, therefore, can be regarded as the worst-case material in terms of the climate-induced risks to parchment. Tensile parameters of parchment varied significantly with increasing relative humidity (RH). Elasticity modulus declined from on average 1200 to 400 MPa and strain at failure doubled when RH increased from 30 to 85%. Parchment’s critical strain at which permanent deformation occurred decreased dramatically with increasing RH reaching zero at 80%. Irreversible curling produced by variations in RH to which flat parchment specimens were subjected were measured by scanning the specimen surface with the use of a laser triangulation sensor. The degree of curling was expressed quantitatively as standard deviation of local curvatures in the parchment sheet. The study opens a perspective of using the relationship between degree of curling and magnitude of RH variations to derive categories of risk to parchment from indoor climate variations, under the condition that quantitative loss of aesthetical/display value of parchment objects resulting from increased curling is agreed. Historical parchment documents generally demonstrating considerable curling engendered by uncontrolled storage conditions in the past are not vulnerable to further distortion when subjected to variations in RH even of considerable magnitude.
RFA measurements of survival midpalatal orthodontic mini-implants in comparison to initial healing period
Authors: Nienkemper Manuel , Willmann Jan H. , Becker Kathrin , Drescher Dieter 
Published: February 2020

Abstract
Background
In dental implantology, the development of stability over time is a well-investigated topic. In case of orthodontic mini-implants, quantitative data for long-term stability is not available yet. This study aims to clinically investigate the long-term stability of mini-implants inserted in the midsagittal suture of the anterior palate. Moreover, the influence of the length of implants was elucidated. The stability of 2 × 9 and 2 × 11 mm mini-implants after orthodontic treatment (9 mm, 2.84 years ± 1.25 years; 11 mm, 3.17 years ± 0.96 years) was assessed by resonance frequency analysis (RFA). The obtained long-term pieces of data were compared with each other (9 mm vs 11 mm), as well as with the data from the matched early stability groups, to assess the initial and early secondary stability after the insertion from previous clinical trials.

Results
For both lengths, the long-term stability (2 × 9 mm, 25.12 ± 7.11, n = 21; 2 × 11 mm, 24.39 ± 5.82, n = 18) was significantly lower than primary stability (2 × 9 mm, 36.14 ± 6.08, n = 19; 2 × 11 mm, 33.35 ± 3.53, n = 20). The differences within the groups disappeared over the initial healing period: after 4 weeks for the 2 × 9 mm implants and after 2 weeks for the 2 × 11 mm implants. Also, the 2 × 9 mm and 2 × 11 mm implants showed comparable long-term stability values.

Conclusion
The stability of midpalatal mini-implants does not change in the long term after the initial healing period. Moreover, 2 × 9 mm mini-implants seem to be appropriate for orthodontic anchorage, as the stability of 2 × 11 mm implants is not higher. Therefore, owing to lower invasiveness, 2 × 9 mm implants should be preferred.
LncRNA SNHG7/miR-34a-5p/SYVN1 axis plays a vital role in proliferation, apoptosis and autophagy in osteoarthritis
Authors: Tian Feng , Wang Junhu , Zhang Zhanhua , Yang Jie 
Published: February 2020

Abstract
Background
Osteoarthritis (OA) is one of the most common rheumatic diseases of which clinical symptoms includes swelling, synovitis and inflammatory pain, affect patients’ daily life. It was reported that non-coding RNAs play vital roles in OA. However, the regulation mechanism of ncRNA in OA pathogenesis has not been fully elucidated.

Methods
The expression of SNHG7, miR-34a-5p and SYVN1 was detected using qRT-PCR in tissues, serum and cells. The protein expression of SYVN1, PCNA, cleavage-caspase 3, beclin1 and LC3 were measured using western blot. The RNA immunoprecipitation (RIP), RNA pulldown, and luciferase reporter assays were used to verify the relationship between SNHG7, miR-34a-5p and SYVN1. The MTT and flow cytometry assay was performed to detected cell proliferation and cell apoptosis respectively.

Results
In this study, SNHG7 and SYVN1 expression were down-regulated, but miR-34a-5p was up-regulated in OA tissues and IL-1β treated cells compared with normal tissues and chondrocyte. Functional investigation revealed that up-regulated SNHG7 or down-regulated miR-34a-5p could promote cell proliferation and inhibit cell apoptosis and autophagy in OA cells. More than that, RIP, pulldown and luciferase reporter assay was applied to determine that miR-34a-5p was a target miRNA of SNHG7 and SYVN1 was a target mRNA of miR-34-5p. Rescue experiments showed that overexpression of miR-34a reversed high expression of SNHG7-mediated suppression of apoptosis and autophagy as well as promotion of proliferation, while its knockdown inhibited cell apoptosis and autophagy and promoted cell proliferation which could be impaired by silencing SYVN1. In addition, SNHG7 regulated SYVN1 through sponging miR-34a-5p.

Conclusion
SNHG7 sponged miR-34a-5p to affect cell proliferation, apoptosis and autophagy through targeting SYVN1 which provides a novel sight into the pathogenesis of OA.
Determinants of stunting among children aged 6 to 59 months in pastoral community, Afar region, North East Ethiopia: unmatched case control study
Authors: Kahssay Molla , Woldu Etsay , Gebre Abel , Reddy Surender 
Published: February 2020

Abstract
Background
Stunting is defined as a child with a height for-age Z-score less than minus two standard deviations. Globally, 162 million less than 5 years were stunted. In Ethiopia, Nationally the prevalence of stunting among under five children was 38.4% and in Afar it is above the national average (41.1%). This study was aimed to identify determinants of stunting among children aged 6 to 59 months in rural Dubti district, Afar region, North East Ethiopia, 2017.

Methods
Community based unmatched case-control study design was conducted among 322 (161 cases and 161 controls) children aged 6 to 59 months from March 2–30/ 2017. Simple random method was used to select 5 kebelles from 13 kebelles. Training was given for data collectors and supervisors. Data were entered to EPI data version 3.02 and exported to SPSS version 20 for analysis. Binary logistic regression analysis was used and variables with p-value < 0.25 on univariable binary logistic regression analysis were further analyzed on multivariable binary logistic regression analysis and statistical significance was declared at 95% CI.

Results
Being from a mother with no education (AOR = 4.92, 95%CI (1.94, 12.4), preceding birth interval less than 24 months (AOR = 4.94, 95% (2.17, 11.2), no ANC follow-up (AOR = 2.81, 95% (1.1.46, 5.38), no access to latrine (AOR =3.26, 95% CI (1.54–6.94), children born from short mother < 150 cm (AOR = 3.75, 95%CI (1.54, 9.18), not fed colostrum (AOR = 4.45, 95% CI (1.68, 11.8), breast fed for less than 24 months (AOR = 3.14, 95% CI (1.7, 5.79) and non-exclusive breast feeding (AOR = 6.68, 95% (3.1, 14.52) were determinants of stunting at 95% CI.

Conclusion
No maternal education, preceding birth interval less than 24 months, no ANC follow-up, no access to latrine, short maternal height, not feeding colostrum, duration of breast feed less than 24 months and non- exclusive breast feeding were determinants of stunting at 95% CI.
Strategic intent of OBOR: enhancing energy supply resilience
Authors: Tang Loon Ching , Low Joyce M.W. 
Published: February 2020

AbstractSince the launch of the One Belt, One Road (OBOR) initiative in 2015, China has announced its intention to invest in major infrastructure projects to promote trade and co-operation with its trading partners along OBOR. This paper examines the current level of trading and key projects underway along OBOR so as to provide insights to understand its strategic intent. In particular, a network model is constructed to analyze the impact to China current and future demand for energy under various conditions especially during prolonged periods of supply uncertainties. Since these key projects are closely connected to the current and proven oil and natural gas reserves locations, the findings propound the OBOR initiative is more than trade and a key motivation is to enhance China’s energy supply resilience amongst other imperatives.
Carbon dioxide angiography during peripheral angioplasty procedures significantly reduces the risk of contrast-induced nephropathy in patients with chronic kidney disease
Authors: Diamantopoulos Athanasios , Patrone Lorenzo , Santonocito Serafino , Theodoulou Iakovos , Ilyas Shazhad , Dourado Renato , Game David , Karunanithy Narayan , Patel Sanjay , Zayed Hany , Katsanos Konstantinos 
Published: February 2020

Abstract
Background
Iodinated contrast media are amongst the most frequently prescribed medications, however, their use is not without complications. With contrast-induced nephropathy constituting a major concern, alternative non-iodine based approaches have been explored such as carbon dioxide angiography. The purpose of this study is to report the incidence of contrast-induced nephropathy following carbon dioxide angiography in patients with impaired renal function that underwent peripheral angioplasty compared with a historical cohort of patients that underwent angioplasty with use of solely iodine contrast medium. The historical cohort of patients treated with iodinated contrast was used as control. Baseline demographics and renal function tests were recorded. Primary outcome was incidence of contrast-induced nephropathy within 48–72 h post intervention. Receiver-Operating-Characteristic curve analysis was used to correlate the volume of iodinated contrast with the risk of contrast-induced nephropathy.

Results
Carbon Dioxide was used as an alternative to iodinated contrast media in patients with impaired renal function (eGFR<60mls/min/1.73 m2) undergoing peripheral angioplasty procedures. Fifty, consecutive patients (baseline eGFR = 38.6 ± 13.2mls/min/1.73 m2) were included in a prospective clinical audit. These were matched (1:2) with a historical cohort of patients (baseline eGFR = 43.3 ± 12.2mls/min/1.73 m2) treated with Iodinated contrast media. The incidence of contrast-induced nephropathy was 14% (n = 7/50) in case of carbon dioxide vs. 29% (n = 29/100) in the matched cohort group (p = 0.045). Receiver-Operating-Characteristic analysis showed that use of >25mls of contrast was 94.4% (95% CI:81–99%) sensitive in predicting contrast-induced nephropathy.

Conclusion
Carbon dioxide imaging during peripheral angioplasty procedures protects against contrast-induced nephropathy. Use of >25mls of iodinated contrast media in high-risk patients is a predictor of contrast-induced nephropathy.
Culture and PCR based detection of bacteria causing urinary tract infection in urine specimen
Authors: Pirkani Ghulam Sarwar , Awan Mohammad Arif , Abbas Ferhat , Din Mohammad 
Published: February 2020

Objectives: Urinary tract infections are the second most common bacterial infections occurring at all ages and both sexes. The increasing rate of antibiotic resistance is a global concern. The use of routinely used antibiotics is resulting in treatment failure. The objective of this study was to diagnose the urinary tract infections by routine culture sensitivity test and by molecular methods.
Methods: This study was conducted in Microbiology laboratory, Bolan Medical Complex Hospital, Quetta, from July 1st to 31st March 2019. Isolates were identified biochemically by API20E & API20NE. Antibiogram was performed using disc diffusion Kirby Bauer technique. The 16S rDNA gene approach was used for molecular identification of bacterial isolates. The presence of the blaNDM-1 gene was identified by polymerase chain reaction (PCR).
Results: We isolated 146 bacterial isolates namely Escherichia coli (n=99) 67.80%, Klebsiella pneumoniae (n=33) 22.60%, Pseudomonas aeruginosa (n=11) 7.53% and Proteus mirabilis (n=3) 2.05% from 2032 urine samples. The resistance pattern was dominated by Multi Drug Resistance (MDR). Remarkably, four isolates of Escherichia coli (n=3) and Klebsiella pneumoniae (n=1) were displaying resistance against a range of antibiotics used in the study, including carbapenems but sensitive to tigecycline and polymyxins only, suggesting extensive drug resistance having blaNDM-1 gene.
Conclusion: This is the first report on direct molecular detection of bacterial pathogens from urinary tract infected patients in Balochistan. The presence of blaNDM-1 in different bacterial species and their extensive drug resistance pattern poses a significant clinical threat. Molecular detection of bacteria and resistant gene may reduce the diagnostic time of patients.
doi: https://doi.org/10.12669/pjms.36.3.1577
How to cite this:Pirkani GS, Awan MA, Abbas F, Din M. Culture and PCR based detection of bacteria causing urinary tract infection in urine specimen. Pak J Med Sci. 2020;36(3):391-395. doi: https://doi.org/10.12669/pjms.36.3.1577
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Diabetes Registry of Pakistan
Authors: Basit Abdul , Fawwad Asher , Baqa Kulsoom 
Published: February 2020

Diabetes registries can be used to monitor the prevalence and incidence of diabetes. Diabetes registries are used in many countries for population management of diabetes, outcomes management, and development of Clinician finding Support Structure, for example, National Diabetes Registry (NDR) in Sweden and Singapore diabetes registry. According to 2nd National Diabetes Survey of Pakistan (NDSP) 2016-2017, overall 26.3% adult (≥20) suffer from diabetes (27.4 million people). Health Research Advisory Board (HRAB) of Pakistan has initiated the mission of developing disease registries countrywide. Diabetes Registry of Pakistan (DROP) under the supervision of Prof. Abdul Basit and Dr. Asher Fawwad to enumerate the degree of the national disease burden. For type 1 diabetes, Diabetes Registry of Pakistan for type 1 (DROP-1) has already started. DROP-1 can be a good pilot arm for developing the robust methodology for DROP. The upcoming and the ongoing research is periodically being linked with the registry to ensure its modernization. This registry is a useful tool for tracking the status of patient, in order to limit the burden of data collection. A web-based data entry system and automated random sampling has enabled useful data collection and tracking with relatively minimal effort.
doi: https://doi.org/10.12669/pjms.36.3.1877
How to cite this:Basit A, Fawwad A, Baqa K. Diabetes Registry of Pakistan. Pak J Med Sci. 2020;36(3):578-580. doi: https://doi.org/10.12669/pjms.36.3.1877
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
The diversity of Anopheles blood feeding patterns suggests different malaria protection strategies in different localities
Authors: Chakim Irfanul , Pumpaibool Tepanata 
Published: February 2020

Background: Malaria is a significant health burden for many countries worldwide. Insecticide-treated bed nets and mosquito repellent are considered effective methods for preventing Anopheles bites. However, changes in the biological properties of the vector have led to a reduction in their effectiveness. Most published studies have only investigated the human population factor, not the dynamics of vector behavior. Therefore, this study aims to investigate the importance of primary vector activity for selecting an appropriate malaria protection strategy. Methods: Initially, active case detection (ACD) was carried out in western and eastern parts of Indonesia, Jambi and Sumba, to confirm their endemicity level. According to the 2016 national health report of Indonesia, Jambi has an annual parasite index (API) of 0.14 and Sumba has an API of 5.41. A series of entomological observations were carried out to compare the biting activity of Anopheles vectors in two localities, with a total of 216 houses and 216 catchers (108 at each study site). Results: The results indicated that endemicity at the sub-district level is higher than that at the provincial level. Only Anopheles balabacensi was found to be exophagic. Multiple comparisons found different biting times between the sites, suggesting that early evening (18.00-20.00) is most likely to be the time when mosquitoes transmit the Plasmodium parasite in Jambi, while during sleeping hours (21.00-01.00) is the peak biting time of Anopheles mosquitoes in Sumba. Conclusions: The study demonstrates the importance of Anopheles species blood feeding patterns in selecting an appropriate malaria protection strategy.
Peptide arrays of three collections of human sera from patients infected with mosquito-borne viruses
Authors: Martinez Viedma Maria del Pilar , Kose Nurgun , Parham Leda , Balmaseda Angel , Kuan Guillermina , Lorenzana Ivette , Harris Eva , Crowe Jr. James E. , Pickett Brett E. 
Published: February 2020

Background: Global outbreaks caused by emerging or re-emerging arthropod-borne viruses (arboviruses) are becoming increasingly more common. These pathogens include the mosquito-borne viruses belonging to the Flavivirus and Alphavirus genera. These viruses often cause non-specific or asymptomatic infection, which can confound viral prevalence studies. In addition, many acute phase diagnostic tests rely on the detection of viral components such as RNA or antigen. Standard serological tests are often not reliable for diagnosis after seroconversion and convalescence due to cross-reactivity among flaviviruses. Methods: In order to contribute to development efforts for mosquito-borne serodiagnostics, we incubated 137 human sera on individual custom peptide arrays that consisted of over 866 unique peptides in quadruplicate. Our bioinformatics workflow to analyze these data incorporated machine learning, statistics, and B-cell epitope prediction. Results: Here we report the results of our peptide array data analysis, which revealed sets of peptides that have diagnostic potential for detecting past exposure to a subset of the tested human pathogens including Zika virus. These peptides were then confirmed using the well-established ELISA method. Conclusions: These array data, and the resulting peptides can be useful in diverse efforts including the development of new pan-flavivirus antibodies, more accurate epitope mapping, and vaccine development against these viral pathogens.
Pathogenic FAM83G palmoplantar keratoderma mutations inhibit the PAWS1:CK1α association and attenuate Wnt signalling.
Authors: Wu Kevin Z.L. , Jones Rebecca A. , Tachie-Menson Theresa , Macartney Thomas J. , Wood Nicola T. , Varghese Joby , Gourlay Robert , Soares Renata F. , Smith James C. , Sapkota Gopal P. 
Published: February 2020

Background: Two recessive mutations in the FAM83G gene, causing A34E and R52P amino acid substitutions in the DUF1669 domain of the PAWS1 protein, are associated with palmoplantar keratoderma (PPK) in humans and dogs respectively. We have previously reported that PAWS1 associates with the Ser/Thr protein kinase CK1α through the DUF1669 domain to mediate canonical Wnt signalling. Methods: Co-immunoprecipitation was used to investigate possible changes to PAWS1 interactors caused by the mutations. We also compared the stability of wild-type and mutant PAWS1 in cycloheximide-treated cells. Effects on Wnt signalling were determined using the TOPflash luciferase reporter assay in U2OS cells expressing PAWS1 mutant proteins. The ability of PAWS1 to induce axis duplication in Xenopus embryos was also tested. Finally, we knocked-in the A34E mutation at the native gene locus and measured Wnt-induced AXIN2 gene expression by RT-qPCR. Results: We show that these PAWS1A34E and PAWS1R52P mutants fail to interact with CK1α but, like the wild-type protein, do interact with CD2AP and SMAD1. Like cells carrying a PAWS1F296A mutation, which also abolishes CK1α binding, cells carrying the A34E and R52P mutants respond poorly to Wnt signalling to an extent resembling that observed in FAM83G gene knockout cells. Consistent with this observation, these mutants, in contrast to the wild-type protein, fail to induce axis duplication in Xenopus embryos. We also found that the A34E and R52P mutant proteins are less abundant than the native protein and appear to be less stable, both when overexpressed in FAM83G-knockout cells and when knocked-in at the native FAM83G locus. Ala34 of PAWS1 is conserved in all FAM83 proteins and mutating the equivalent residue in FAM83H (A31E) also abolishes interaction with CK1 isoforms. Conclusions: We propose that mutations in PAWS1 cause PPK pathogenesis through disruption of the CK1α interaction and attenuation of Wnt signalling.
Root Caries Part 1: an Overview of the Challenges
Authors: Burrows Stephen 
Published: February 2020

The elderly population is increasing and they have a higher retention of natural teeth than previous cohorts. The increased prevalence of root caries in this group presents challenges to all those concerned in its management. In this article, aetiology, risk factors and diagnosis of root caries are discussed with the aim of implementing effective approaches in its management. CPD/Clinical Relevance: Caries control should, when possible, be non-operative.