bluuebunny/crossref_metadata_2025_split huggingface dataset:
Precise Regulation and Site-specifically Covalent Labeling of NSUN2 Enable by Genetic Encoding Expansion
Authors: Zhao Jizhong , Hu Hongmei , Wang Sheng , Wang Li , Wang Rui 
Published: December 2020

ABSTRACTRNA plays a critical role in gene expression regulation, cell migration, differentiation, cell death in living organism. 5-Methylcytosine is a post transcriptional RNA modification identified across wide ranges of RNA species including message RNAs. It is reported the addition of m5C to RNA cytosines is enabled by use of NSUN family enzyme, NSUN2 is identified as a critical RNA methyltransferase for adding m5C to mRNA. We demonstrated here that natural lysines modified with special groups were synthesized via chemical synthesis. Through two rounds of positive screening and one round of negative screening, MbPylRS-tRNAcua unnatural lysine substitution system which can specifically recognize lysine with defined group was evaluated and identified. Non-natural lysine substitution at C271 of NSUN2 active site and subsequently fluorescent labeling was realized via so-called click reaction. The function of NSUN2 mutant and its upregulated CDK1 gene and its effect on cell proliferation were also evaluated.
In Silico Design and Analysis of Genetic Circuit-Based Whole-Cell Biosensors for Detecting Groundwater Contaminants
Authors: Fajemilua Samuel , Bada Solomon , Islam M. Ahsanul 
Published: December 2020

AbstractContaminants of emerging concern (CEC) such as tetracycline, erythromycin, and salicylic acid in groundwater can seriously endanger the environment and human health due to their widespread and everlasting harmful effects. Thus, continuous monitoring of various CEC concentrations in groundwater is essential to ensure the safety, security, and biodiversity of natural habitats. CECs can be detected using whole-cell biosensors for environmental surveillance and monitoring purposes, as they provide a cheaper and more robust alternative to traditional and expensive analytical techniques. In this study, various genetic circuit designs are considered to model three biosensors using the genetic design automation (GDA) software, iBioSim. The genetic circuits were designed to detect multiple CECs, including atrazine, salicylic acid, and tetracycline simultaneously to produce quantitative fluorescent outputs. The biosensor responses and the viability of the genetic circuit designs were further analysed using ODE-based mathematical simulations in iBioSim. The designed circuits and subsequent biosensor modelling presented here, thus, not only show the usefulness and importance of GDA tools, but also highlight their limitations and shortcomings that need to overcome in the future; thereby, providing a practical guidance for further improvement of such tools, so that they can be more effectively and routinely used in synthetic biology research.
Investigating stewardship behavior in megaprojects: An exploratory analysis
Authors: Ma Tianyu , Wang Zhuofu , Skibniewski Miroslaw Jan , Ding Jiyong , Wang Ge , He Qinghua 
Published: December 2020

PurposeThis research aims to analyzes how megaproject top managers engaged in stewardship behaviors. Studying megaprojects from the micro-foundations rooted in individual action and interaction, this research examines the gaps between literature and top managers' positive behavior to challenge the current theoretical underpinnings of megaproject governance research and practice.Design/methodology/approachAn extensive literature review was performed in the initial phase. Then, a case study of South-to-North Water Diversion project was conducted based on following this project and on access to its top executives. Data was collected from multiple sources and analyzed by Nvivo (version 12). Further analysis was then carried out in two stages to identify megaproject stewardship behavior and related governance patterns.FindingsResults show that stewardship behavior is prevalently existing and is possibly to be identified through psychological, situational, relational dimensions. Also, 16 factors have been found to describe the precise nature of megaproject stewardship behavior. Further explorative findings were discussed from three perspectives: possible theoretical development, self-actualization motivation and temporalities of megaprojects.Originality/valueBuilding upon the ideas on how to extend steward theory towards project field, this research conducts a first exploration of stewardship behavior in megaprojects. This study contributes to complement the research into top-level organizational behavior in megaprojects, and it provides helpful implications for how to govern top managers in the following megaprojects with the cooperative spirit that can be valued by megaproject stakeholders.
Analysing reader behaviours in self-service library stations using a bibliomining approach
Authors: Tu Yun-Fang , Chang Shao-Chen , Hwang Gwo-Jen 
Published: December 2020

Purpose
The present study aims, by adopting bibliomining, to analyse the borrowing and collection records in self-service libraries at mass rapid transit stations in northern Taiwan to discover reader borrowing preferences and patterns.

Design/methodology/approach
The current study used data mining to analyse two years of book-borrowing information from self-service library stations; it made use of an association rule mining model and the bibliomining process to identify readers’ preferred books and to explore reader borrowing behaviours. In addition, the librarians’ perceptions of the proposed approach were also investigated.

Findings
The findings indicated that readers often borrowed books in the bibliographical classifications of Home economics; Medical sciences; Psychology; Commerce: administration and management; and Education in the self-service library stations. Based on the bibliomining results, 23 reader borrowing patterns as well as potential books favoured by readers were uncovered. In addition, the challenges of bibliomining and data mining applied to library operations are reported.

Originality/value
Among the studies on the application of self-service technologies in libraries, most examined the integration of the self-service system and investigated users’ opinions. The present study used borrowing records and collection records in self-service library stations to conduct bibliomining and to explore reader borrowing preferences and behaviours as references for collection development and book recommendation services.
Some like it hot? Elite female athlete perspectives on competing in extreme climatic conditions at world championship sports events
Authors: Zeiler Pauline , Shipway Richard 
Published: December 2020

PurposeThis paper explores perspectives of elite female athletes competing at world championship sports events, hosted in extreme climatic conditions. From the athlete perspective, it examines the implications of decisions by global sports federations when selecting host cities and the subsequent impacts upon elite athletes competing in unfavourable climates.Design/methodology/approachUsing an exploratory case study approach at the 2019 IAAF World Athletics Championships in Doha, Qatar, this paper explores insider perspectives of four elite female participants competing in the Marathon. Adopting the “elite interviewing” technique, and based on event participant experiences, the paper prioritises the perspective and “voice” of the athlete.FindingsFour key themes emerged from the data. These were (1) the importance of training and preparation; (2) unique challenges of competition day; (3) elite athlete retrospection; and (4) prioritising the perspectives of elite athletes at international sports events. The findings highlight the challenges of managing athlete preparation and performance at world championship events hosted in extreme climatic conditions.Research limitations/implicationsThe sample size for this study whilst relatively small is unique. This reflects challenges accessing elite athletes, their reluctance to be interviewed, and the small pool of elite athlete talent available. Given these practical barriers, this represents a good sample size.Originality/valueThis paper secures unique insider access to elite level female endurance athletes competing at world championship events. The study makes a contribution to knowledge in event studies by introducing concepts of “elite event interviewing” and the “athlete-event paradox”.
Assessing the telemedicine acceptance for adults in Brazil
Authors: Serrano Karina M. , Mendes Glauco H.S. , Lizarelli Fabiane L. , Ganga Gilberto M.D. 
Published: December 2020

PurposeThis study aimed to find factors influencing the acceptance of telemedicine for adults in Brazil. Moreover, it investigates the moderating role of disease complexity and the generational digital divide phenomenon on the intention to use the telemedicine service.Design/methodology/approachA quasi-experiment was employed. Primary data were collected using a survey research method considering two different scenarios based on disease complexity and symptom severity. A total of 248 responses were collected using a structured questionnaire. The authors also tested these two scenarios in three generations (X, Y and Z) of adults. Partial least squares structural equation modeling (PLS-SEM) was used to analyze the collected data and test the research hypotheses.FindingsThe results indicated that performance expectancy and perceived security and reliability are two predictors of the behavioral intention to use telemedicine, whereas effort expectancy and social influence showed no statistical significance. Furthermore, the results demonstrated that adults tend to adopt telemedicine regardless of the level of disease complexity. Finally, this study does not support the existence of a digital divide in the three generations.Originality/valueThe study applies the UTAUT model to assess the telemedicine acceptance for younger generations. It examines patient risk perception (security and reliability) as one antecedent of telemedicine acceptance.
Home Care Providers in Queensland: Exploratory Data Analysis Using My Aged Care Platform
Authors: (Michelle) Tran My , Gannon Brenda 
Published: December 2020

AbstractThe My Aged Care website, established by the Australian Government, is the starting point for aged care consumers and carers to find information on aged care providers. Using information published on this platform, we compile a unique dataset of 588 approved home care providers in Queensland. This article presents a brief introduction to this dataset, including how they are extracted and what variables are available. We propose some current and future applications of this data with a focus on how it can contribute to evidence‐based policymaking in Australia.
Debt and Deficits—A Modern Monetary Theory Perspective
Authors: Mitchell William F. 
Published: December 2020

AbstractCOVID‐19 has triggered the most severe economic crisis since the 1930s. The resulting policy shift is diametric to what mainstream macroeconomists have been advocating for decades. We argue that their framework underestimates the fiscal space available to governments and cannot provide an understanding of the consequences of these policy extremes. We introduce modern monetary theory (MMT), which disabuses us of the claims that deficits and debt are to be avoided. MMT defines fiscal space in functional terms, in relation to available real resources that can be brought back into productive use, rather than focusing on irrelevant questions of government insolvency.
Ethical and professionalism issues in dermatopathology: A cross‐sectional survey of American Society of Dermatopathology Members
Authors: Brahmbhatt Meera , Yeung Howa , Vong Gerard , Allbritton Jill , Amin Bijal , Dulaney Eugene D. , Fullen Douglas R. , LeBlanc Robert E. , Ruben Beth S. , Seykora John T. , Vyas Nikki S. , Stoff Benjamin K. 
Published: December 2020

AbstractBackgroundData regarding ethical/professional issues affecting dermatopathologists are lacking despite their importance in establishing policy priorities and educational content for dermatopathology.MethodsA 14‐item cross‐sectional survey about ethical/professional issues in dermatopathology was distributed over e‐mail to members of the American Society of Dermatopathology from June to September 2019.ResultsTwo hundred sixteen surveys were completed, with a response rate of 15.3%. Respondents ranked appropriate and fair utilization of healthcare resources (n = 83 or 38.6%) as the most often encountered ethical/professional issue. Conflict of interest was ranked as the most urgent or important ethical/professional issue (n = 83 or 39.3%). One hundred thirty‐three (61.6%) respondents felt “somewhat” or “not at all” well equipped to handle ethical dilemmas in practice and 47 (22.8%) respondents identified a major or extreme burden (eg, have considered resigning/retiring) due to ethical challenges.ConclusionsAreas of priority in ethics and professionalism issues can guide future policy and educational content in dermatopathology.
Different pre‐analytical techniques and the results of 50 g oral glucose challenge tests
Authors: Eldem Sinem , Oskovi‐Kaplan Z. Asli , Engin‐Ustun Yaprak , Yilmaz Canan , Caglar Ali Turhan , Ozgu‐Erdinc A. Seval 
Published: December 2020

AbstractObjectiveWe aimed to analyse the pre‐analytical process and its effect of 50 g of oral glucose challenge test results for screening gestational diabetes mellitus.Research Design and MethodsThe 50 g oral glucose challenge test was performed to 30 pregnant women, and the blood was collected as two samples for three tubes containing; serum separating jell (SSJ), sodium fluoride‐potassium oxalate (NaF – KOx) and sodium citrate‐containing tube. The first samples of the three tubes were centrifuged within 30 minutes, and second samples were centrifuged after 60 minutes and were analysed. One sample in SSJ tube and was analysed in the same day according to hospitals routine practice. The results were compared.ResultsAmong the 30 samples, the mean decrease in glucose levels was highest in the SSJ tube (0.38 mmol/L), followed by 0.16 mmol/L in Na citrate tube and 0.14 mmol/L in NaF‐KOx tube. The hospital routine assessment with SSJ was 6.36 ± 1.90 mmol/L. The <30 and >60 minutes glucose results were 6.80 ± 1.88 mmol/L vs 6.42 ± 1.97 mmol/L for SSJ, 5.95 ± 1.60 mmol/L vs 5.78 ± 1.51 mmol/L for Na Citrate and 6.90 ± 1.86 mmol/L vs 6.75 ± 1.90 mmol/L for NaF‐KOx mg/dL groups, respectively, and both the changes within time and the results between the tubes showed a statistically significant difference (P < .001).ConclusionIn cases with longer assessment time and with different blood sample tubes, the clinician should also keep in mind that, especially with results under but close to the cut‐off levels, an underdiagnosed gestational diabetes might be present.
Bloodmeal‐stealing in wild‐caught Mepraia spinolai (Hemiptera: Reduviidae), a sylvatic vector of Trypanosoma cruzi
Authors: Garrido Rubén , Campos‐Soto Ricardo , Quiroga Nicol , Botto‐Mahan Carezza 
Published: December 2020

1. Blood‐feeding bugs in the Triatominae are the vectors of Trypanosoma cruzi, the etiological agent of Chagas disease. Mepraia is a sylvatic genus endemic to Chile that transmits T. cruzi in the wild cycle.2. Bloodmeal‐stealing (or ‘cleptohaematophagy’) is the stealing of a bloodmeal from one bug's gut by another, usually conspecific bug. Bloodmeal‐stealing can result in horizontal transmission of T. cruzi between triatomines; so far, it has been reported only in laboratory‐reared bugs.3. We performed short laboratory experiments to test whether bloodmeal‐stealing occurs between wild‐caught Mepraia spinolai nymphs. Successful bloodmeal‐stealing was observed in one out of 17 trials (2/102 bugs). Even though bloodmeal‐stealing was not frequent in wild‐caught M. spinolai, this behaviour might contribute to explaining the maintenance of wild T. cruzi cycles.
The natural persistence and distribution of the proposed biological control agent Verticillium nonalfalfae on Ailanthus altissima in Virginia, USA
Authors: Brooks Rachel K. , Baudoin Anton , Salom Scott M. 
Published: December 2020

AbstractReports of Ailanthus altissima stand declines in south‐central Pennsylvania resulted in the identification of the causal agent, a vascular wilt fungus, Verticillium nonalfalfae. Additional surveys throughout North America and Europe found that this disease outbreak was not an isolated event. One of these surveys identified and monitored six naturally diseased A. altissima stands in Virginia, USA, between 2009 and 2012. To help predict the long‐term outcome of infested stands, these naturally infested sites were monitored again from 2017 to 2018. While A. altissima were still present at all six sites, a reduction in the number of living A. altissima per hectare (from 604 to 66 overall) and A. altissima basal area per hectare (from 16.1 to 2.9 m2/ha overall) was consistently seen. At all but one of these sites, V. nonalfalfae was still causing disease on the remaining A. altissima. This reduction in A. altissima and persistence of V. nonalfalfae highlight the effectiveness of the pathogen at controlling this tree over long periods of time. A walking survey looking closely at all A. altissima stands along roads within 1 km of these sites found additional V. nonalfalfae infections around four sites. Since no correlation existed between tree infection status and distance from the known site, the patchy disease occurrence may indicate this pathogen is able to spread over longer distances or is more abundant than the previous survey suggested. Soil collected at these sites did not infect any A. altissima seedlings in a greenhouse study, suggesting that soil inoculum may not play an important role in V. nonalfalfae persistence. This research further supports the use of V. nonalfalfae as an effective biopesticide that can reduce A. altissima numbers consistently over many years.
Diagnostic markers for Teratosphaeria destructans and closely related species
Authors: Havenga Minette , Wingfield Brenda D. , Wingfield Michael J. , Dreyer Léanne L. , Roets Francois , Aylward Janneke 
Published: December 2020

AbstractTeratosphaeria foliar pathogens cause leaf and shoot blight on Eucalyptus trees in many parts of the world. Among them, T. destructans is one of the most aggressive pathogens causing defoliation of young Eucalyptus trees in tropic regions. Identification of T. destructans to species level is currently not possible based solely on morphological characteristics or ITS sequence data. The aim of this study was to assess T. destructans microsatellites and a newly developed T. epicoccoides microsatellite as a diagnostic tool to differentiate among T. destructans and several closely related foliar pathogens. Based on the number of markers that amplified, the T. destructans microsatellites allowed for the differentiation of T. destructans, T. epicoccoides,T. eucalypti,T. nubilosa,T. pseudoeucalypti and T. viscidus. These microsatellites provide a rapid and cost‐effective diagnostic tool that will enable the identification of a large number of isolates important in disease surveys and inoculation trials.
A critical appraisal on the recurrence of sandalwood spike disease and its management practices
Authors: Mondal Soma , Sundararaj Ramachandran , Yashavantha Rao H. C. 
Published: December 2020

AbstractSandal (Santalum album) is an important forest tree that has been utilized for centuries, valued for its wood and scented essential oil derived from the heartwood. Sandalwood spike disease (SSD) is one of the most destructive problems that affect Indian sandalwood. In 1969, three research groups confirmed that the causal agent of SSD was a phytoplasma. Although research literature on sandalwood spike disease has been published for over a century, many earlier publications reported only primarily descriptive macro‐morphological data. Identification of the causal agent was confirmed only recently, using nested PCR of 16S rRNA‐encoding gene sequences and restriction fragment length polymorphism (RFLP) analysis. The identified phytoplasma exhibited high sequence similarity to the aster yellows subgroup 16SrI‐B in NCBI BLAST search. The use of tissue culture in transgenic and disease‐free cloning strategies to produce disease‐resistant sandalwood tress might be the most realistic solution for SSD. The aim of this review is to increase understanding of SSD among phytopathologists in order to encourage research on SSD to discover best management practices. The increased cultivation of sandalwood plantations has significant importance, and it is essential to review the status of SSD.
L2 learners' language attitudes and their assessment
Authors: Artamonova Tatiana 
Published: December 2020

AbstractThis article describes the development of a new questionnaire for assessing L2 learners' language attitudes. Drawing on theoretical work in the fields of social psychology and applied linguistics, the author reviewed the concept of (language) attitudes and contrasted them with the concept of (language) motivation. This thorough literature review, including an examination of the currently available instruments, resulted in the generation of question items for the new questionnaire. The empirical part of the project consisted of data collection and analyses to establish the internal structure of the proposed instrument and to assess its reliability. The new Language Attitudes Questionnaire for Language Learners was found psychometrically sound and easily interpretable.
Non‐KIR NK cell receptors: Role in transplantation of allogeneic haematopoietic stem cells
Authors: Bogunia‐Kubik Katarzyna , Łacina Piotr 
Published: December 2020

AbstractNatural killer (NK) cells are of major significance in patients after allogeneic haematopoietic stem cell transplantation (HSCT). They are the first subset of lymphocytes to appear in peripheral blood after transplantation and play an important role in the immune responses against cancer and viral infections. The function of NK cells is controlled by various surface receptors, of which type I integral proteins with immunoglobulin‐like domains (killer‐cell immunoglobulin‐like receptors, KIRs) have been the most extensively studied. The present review focuses on less studied NK cell receptors, such as type II integral proteins with lectin‐like domains (CD94/NKG2, NKG2D), natural cytotoxicity receptors (NCRs), immunoglobulin‐like transcripts (ILTs) and their ligands. Their potential role in patients with haematological disorders subjected to HSC transplant procedure in the context of post‐transplant complications such as viral reactivation and acute graft‐versus‐host disease (GvHD) will be presented and discussed.