bluuebunny/crossref_metadata_2025_split huggingface dataset:
Selective dysregulation of ROCK2 activity promotes aberrant transcriptional networks in ABC diffuse large B-cell lymphoma
Authors: Ricker Edd , Verma Akanksha , Marullo Rossella , Gupta Sanjay , Ye Chao , Pannellini Tania , Manni Michela , Tam Wayne , Inghirami Giorgio , Elemento Olivier , Cerchietti Leandro , Pernis Alessandra B. 
Published: August 2020

AbstractActivated B-cell-like diffuse large B-cell lymphoma (ABC-DLBCL) is an aggressive subtype of lymphoma usually associated with inferior outcomes. ABC-DLBCL exhibits plasmablastic features and is characterized by aberrancies in the molecular networks controlled by IRF4. The signaling pathways that are dysregulated in ABC-DLBCL are, however, not fully understood. ROCK2 is a serine-threonine kinase whose role in lymphomagenesis is unknown. Here we show that ROCK2 activity is constitutively dysregulated in ABC-DLBCL but not in GCB-DLBCL and BL. We furthermore show that ROCK2 phosphorylates IRF4 and that the ROCK2-mediated phosphorylation of IRF4 modulates its ability to regulate a subset of target genes. In addition to its effects on IRF4, ROCK2 also controls the expression of MYC in ABC-DLBCL by regulating MYC protein levels. ROCK inhibition furthermore selectively decreases the proliferation and survival of ABC-DLBCL in vitro and inhibits ABC-DLBCL growth in xenograft models. Thus, dysregulated ROCK2 activity contributes to the aberrant molecular program of ABC-DLBCL via its dual ability to modulate both IRF4- and MYC-controlled gene networks and ROCK inhibition could represent an attractive therapeutic target for the treatment of ABC-DLBCL.
Ruminal volatile fatty acid absorption is affected by elevated ambient temperature
Authors: Bedford Andrea , Beckett Linda , Harthan Laura , Wang Chong , Jiang Ning , Schramm Hollie , Guan Le Luo , Daniels Kristy M. , Hanigan Mark D. , White Robin R. 
Published: August 2020

AbstractThe objective of this study was to investigate the effect of short-term elevated ambient temperature on ruminal volatile fatty acid (VFA) dynamics and rumen epithelium gene expression associated with the transport and metabolism of VFA. Eight ruminally cannulated Holstein heifers (200 kg) were used in a factorial, repeated measures experiment with two treatments and two periods. During the first period, animals were provided with feed ad libitum and housed at 20 °C. During the second period, one group (HS) was housed at 30 °C and fed ad libitum. The other group (PF) was housed at 20 °C and pair-fed to match the intake of the HS group. During each period, animals were kept on treatment for 10 day, with sample collection on the final day. In the second period, indicators of heat stress were significantly different between PF and HS animals (P < 0.05). There was a thermal environment effect on butyrate production (P < 0.01) that was not associated with feed intake (P = 0.43). Butyrate absorption decreased in HS animals (P < 0.05) but increased in PF animals (P < 0.05) from period 1 to period 2. There was a feed intake effect on BHD1 expression (P = 0.04) and a tendency for a thermal environment effect (P = 0.08), with expression increasing in both cases. Expression of MCT4 was affected by feed intake (P = 0.003) as were all NHE genes (NHE1, NHE2, and NHE3; P < 0.05). These results indicate that with low feed intake and heat stress, there are shifts in rumen VFA dynamics and in the capacity of the rumen epithelium to absorb and transport VFA.
Systematized reporter assays reveal ZIC protein regulatory abilities are Subclass-specific and dependent upon transcription factor binding site context
Authors: Ahmed Jehangir N. , Diamand Koula E. M. , Bellchambers Helen M. , Arkell Ruth M. 
Published: August 2020

AbstractThe ZIC proteins are a family of transcription regulators with a well-defined zinc finger DNA-binding domain and there is evidence that they elicit functional DNA binding at a ZIC DNA binding site. Little is known, however, regarding domains within ZIC proteins that confer trans-activation or -repression. To address this question, a new cell-based trans-activation assay system suitable for ZIC proteins in HEK293T cells was constructed. This identified two previously unannotated evolutionarily conserved regions of ZIC3 that are necessary for trans-activation. These domains are found in all Subclass A ZIC proteins, but not in the Subclass B proteins. Additionally, the Subclass B proteins fail to elicit functional binding at a multimerised ZIC DNA binding site. All ZIC proteins, however, exhibit functional binding when the ZIC DNA binding site is embedded in a multiple transcription factor locus derived from ZIC target genes in the mouse genome. This ability is due to several domains, some of which are found in all ZIC proteins, that exhibit context dependent trans-activation or -repression activity. This knowledge is valuable for assessing the likely pathogenicity of variant ZIC proteins associated with human disorders and for determining factors that influence functional transcription factor binding.
Fast Periodic Visual Stimulation indexes preserved semantic memory in healthy ageing
Authors: Milton Alex , Rowland Alesi , Stothart George , Clatworthy Phil , Pennington Catherine M. , Kazanina Nina 
Published: August 2020

AbstractBehavioural studies investigating the preservation of semantic memory in healthy ageing have reported mixed findings. One suggested reason for this discrepancy is that the processes underpinning lexical access to semantic knowledge may be sensitive to ageing. It is therefore necessary to assess semantic memory utilising tasks that are not explicitly linguistic. In this study, a fast periodic visual stimulation (FPVS) paradigm coupled with EEG was used to assess the ability of younger and older adults to automatically distinguish between images by their semantic category. Participants were presented with a 6 Hz stream of images drawn from one semantic category except every fifth image (occurring at a rate of 1.2 Hz) which was drawn from an alternate semantic category. For both younger and older adults, results demonstrate successful and comparable semantic categorisation. This was detectable at the individual level for 71% and 72% of older and younger adults, respectively. Given the rapid presentation rate and absence of explicit instruction to categorise images, the task is unlikely to utilise linguistic strategies and suggests the maintenance of semantic memory in healthy ageing. Moreover, this study utilised mobile EEG equipment and short presentation times that would be suitable for practical application outside a research setting.
Porcine reproductive and respiratory syndrome virus infection induces endoplasmic reticulum stress, facilitates virus replication, and contributes to autophagy and apoptosis
Authors: Chen Quangang , Men Yanjuan , Wang Dang , Xu Deqin , Liu Suyan , Xiao Shaobo , Fang Liurong 
Published: August 2020

AbstractDuring viral infection, the host cell synthesizes high amounts of viral proteins, which often causes stress to the endoplasmic reticulum (ER). To manage abnormal ER stress, mammalian cells trigger a response called the unfolded protein response (UPR). Previous studies have indicated that porcine reproductive and respiratory syndrome virus (PRRSV), an Arterivirus that has been devastating the swine industry worldwide, can induce ER stress and activate UPR, however, the activation pathways and the biological significance requires further investigation. In this study, we demonstrated that, among the three types of UPR pathways, PRRSV infection induced PERK and IRE1 pathways, but not the ATF6 pathway. Furthermore, the induction of UPR promoted PRRSV replication. We also found that PRRSV-induced UPR, particularly the PERK pathway, was involved in the induction of autophagy, a cellular degradation process that can alleviate cell stress. Besides, we also provided insights into the ER stress-mediated apoptosis in response to PRRSV infection. PRRSV infection induced the expression of the transcription factor CHOP, which activated caspase 3 and PARP led to ER stress-mediated apoptosis. Using 3-Methyladenine (3-MA) to inhibit autophagy, the increased ER stress and cell apoptosis were observed in the PRRSV infected cell. Taken together, our results revealed the associations of ER stress, autophagy, and apoptosis during PRRSV infection, helping us to further understand how PRRSV interacts with host cells.
Differential effects of ethanol on behavior and GABAA receptor expression in adult zebrafish (Danio rerio) with alternative stress coping styles
Authors: Goodman Alexander C. , Wong Ryan Y. 
Published: August 2020

AbstractVariation in stress responses between individuals are linked to factors ranging from stress coping styles to sensitivity of neurotransmitter systems. Many anxiolytic compounds (e.g. ethanol) can increase stressor engagement through modulation of neurotransmitter systems and are used to investigate stress response mechanisms. There are two alternative suites of correlated behavioral and physiological responses to stressors (stress coping styles) that differ in exploration tendencies: proactive and reactive stress coping styles. By chronically treating individuals differing in stress coping style with ethanol, a GABA-acting drug, we assessed the role of the GABAergic system on the behavioral stress response. Specifically, we investigated resulting changes in stress-related behavior (i.e. exploratory behavior) and whole-brain GABAA receptor subunits (gabra1, gabra2, gabrd, & gabrg2) in response to a novelty stressor. We found that ethanol-treated proactive individuals showed lower stress-related behaviors than their reactive counterparts. Proactive individuals showed significantly higher expression of gabra1, gabra2, and gabrg2 compared to reactive individuals and ethanol treatment resulted in upregulation of gabra1 and gabrg2 in both stress coping styles. These results suggest that impacts of ethanol on stress-related behaviors vary by stress coping style and that expression of select GABAA receptor subunits may be one of the underlying mechanisms.
Differential proteomics study of postharvest Volvariella volvacea during storage at 4 °C
Authors: Zha Lei , Chen Mingjie , Yu Changxia , Guo Qian , Zhao Xu , Li Zhengpeng , Zhao Yan , Li Chuanhua , Yang Huanling 
Published: August 2020

AbstractThe postharvest storage of Volvariella volvacea is an important factor limiting the industry development. Low-temperature storage is the traditional storage method used for most edible fungi, but V. volvacea undergoes autolysis at low temperature. To understand the molecular mechanism underlying the low-temperature autolysis of V. volvacea after harvesting, fruiting bodies of V. volvacea strain V23 were stored at 4 °C. Based on our previous study, in which the changes of morphological and physiological indexes during storage for 0, 6, 12, 24, 30, 36, 48 and 60 h were measured; four time points, namely, 0, 12, 24 and 60 h, were selected for this differential proteomics study. The proteomic changes in the postharvest storage samples were studied by isobaric tags for relative and absolute quantification-coupled two-dimensional liquid chromatography-tandem mass spectrometry (2D LC–MS/MS). A total of 2,063 proteins were identified, and 192 differentially expressed proteins (DEPs), including 24 up-regulated proteins and 168 down-regulated proteins, were detected after 12 h of storage. After 24 h of storage, 234 DEPs, including 48 up-regulated and 186 down-regulated proteins, were observed, and after 60 h, 415 DEPs, including 65 up-regulated proteins and 350 down-regulated proteins, were observed. An in-depth data analysis showed that the DEPs participated in various cellular processes, particularly metabolic processes. In this study, we combined Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses, and the results focused on oxidative phosphorylation and ubiquitin mediated proteolysis pathways. In addition, sdh2, uba1 and ubc1 was confirmed by quantitative real-time polymerase chain reaction, and the results showed that the expression of these genes were consistent with their protein level. Based on the literature and our results, it is speculated that the identified DEPs, such as ATP1, SDH2, COR1, UBA1, COX4, UBC1 and SKP1 play a key role in the low-temperature autolysis of V. volvacea.
Extreme hyperthermia tolerance in the world’s most abundant wild bird
Authors: Freeman M. T. , Czenze Z. J. , Schoeman K. , McKechnie A. E. 
Published: August 2020

AbstractThe thermal tolerances of vertebrates are generally restricted to body temperatures below 45–47 °C, and avian and mammalian critical thermal maxima seldom exceed 46 °C. We investigated thermoregulation at high air temperatures in the red-billed quelea (Quelea quelea), an African passerine bird that occurs in flocks sometimes numbering millions of individuals. Our data reveal this species can increase its body temperature to extremely high levels: queleas exposed to air temperature > 45 °C increased body temperature to 48.0 ± 0.7 °C without any apparent ill-effect, with individual values as high as 49.1 °C. These values exceed known avian lethal limits, with tolerance of body temperature > 48 °C unprecedented among birds and mammals.
Integrated graphene oxide resistive element in tunable RF filters
Authors: Abunahla Heba , Gadhafi Rida , Mohammad Baker , Alazzam Anas , Kebe Mamady , Sanduleanu Mihai 
Published: August 2020

AbstractAdaptable communication systems are of great interest as they provide dynamic front end to accommodate the tunable spectrum management in advanced wireless systems. Memristor (acronym of memory resistor) is an emerging technology part of resistive RAM (RRAM) that has good potential for application in reconfigurable RF devices. The potentiality of using resistive switches for frequency tuning of high frequency RF filters is successfully explored in this article for the first time. Tunable RF filter is designed with detailed simulation using Ansys HFSS, and then correlated with measured results from experiment. As a proof of concept, a prototype of the tunable RF filter is fabricated by using a graphene oxide (GO) integrated with a conventional microstrip open stub notch filter. The resistor switching ability of the device is exploited for the frequency tuning. The resonating length of the notch filter is varied by changing the resistance of the active GO material between ‘HIGH’ (OFF) and ‘LOW’ (ON) resistance states. The measured results demonstrate the great potential of using RRAM in tunable RF devices. It also proves the possibility of tuning RF devices without any localized surface mount device (SMD) element or complex realization technique.
Neurological disorders-associated anti-glycosphingolipid IgG-antibodies display differentially restricted IgG subclass distribution
Authors: Lardone Ricardo D. , Irazoqui Fernando J. , Nores Gustavo A. 
Published: August 2020

AbstractAntibodies against several self-glycans on glycosphingolipids are frequently detected in different neurological disorders. Their pathogenic role is profusely documented, but the keys for their origin remain elusive. Additionally, antibodies recognizing non-self glycans appear in normal human serum during immune response to bacteria. Using HPTLC-immunostaining we aimed to characterize IgM and IgG subclass antibody responses against glycosphingolipids carrying self glycans (GM1/GM2/GM3/GD1a/GD1b/GD3/GT1b/GQ1b) and non-self glycans (Forssman/GA1/“A” blood group/Nt7) in sera from 27 randomly selected neurological disorder patients presenting IgG reactivity towards any of these antigens. Presence of IgG2 (p = 0.0001) and IgG1 (p = 0.0078) was more frequent for IgG antibodies against non-self glycans, along with less restricted antibody response (two or more simultaneous IgG subclasses). Contrariwise, IgG subclass distribution against self glycans showed clear dominance for IgG3 presence (p = 0.0017) and more restricted IgG-subclass distributions (i.e. a single IgG subclass,p = 0.0133). Interestingly, anti-self glycan IgG antibodies with simultaneous IgM presence had higher proportion of IgG2 (p = 0.0295). IgG subclass frequencies were skewed towards IgG1 (p = 0.0266) for “anti-self glycan A” subgroup (GM2/GM1/GD1b) and to IgG3 (p = 0.0007) for “anti-self glycan B” subgroup (GM3/GD1a/GD3/GT1b/GQ1b). Variations in players and/or antigenic presentation pathways supporting isotype (M-G) and IgG-subclass pattern differences in the humoral immune response against glycosphingolipids carrying non-selfversusself-glycans are discussed.
25-Hydroxyvitamin D status, vitamin D intake, and skin cancer risk: a systematic review and dose–response meta-analysis of prospective studies
Authors: Mahamat-Saleh Yahya , Aune Dagfinn , Schlesinger Sabrina 
Published: August 2020

AbstractSun exposure is a major environmental risk factor for skin cancers and is also an important source of vitamin D. However, while experimental evidence suggests that vitamin D may have a protective effect on skin cancer risk, epidemiologic studies investigating the influence of 25-hydroxyvitamin D (25(OH)D) level and/or vitamin D intake on skin cancer risk are conflicting. A systematic review and dose–response meta-analyses of prospective studies was conducted to clarify these associations. Relevant studies were identified by searching the PubMed database up to 30th August 2019. Random effects dose–response meta-analyses were used to estimate summary relative risks (SRRs) and 95% confidence intervals (CIs). Overall, thirteen prospective studies were included. Circulating level of 25(OH)D was associated with higher risks of melanoma (SRR (95% CI) per 30 nmol = 1.42 (1.17–1.72)) and keratinocyte cancer (KC) (SRR (95% CI) per 30 nmol/L = 1.30 (1.13–1.49)). The SRR (95% CI) per 30 nmol/L increase in 25(OH) D level was 1.41 (1.19–1.67), and 1.57 (0.64–3.86), for basal cell carcinomas (BCCs) and squamous cell carcinomas (SCCs), respectively. However, while we found that vitamin D intake (from diet, supplemental and total) was not associated with risks of melanoma and SCC, vitamin D intake was associated with slightly increased BCC risk, albeit with no heterogeneity across skin cancer type. This meta-analysis suggests positive associations between circulating 25(OH)D level and risk of melanoma and KC, however, this finding is most likely confounded by sun exposure. We found no associations between vitamin D intake skin cancers, except positive associations with BCC risk.
An early Pangaean vicariance model for synapsid evolution
Authors: Brikiatis Leonidas 
Published: August 2020

AbstractGenetic isolation due to geographic separation (vicariance) is the best understood cause of vertebrate speciation. Nevertheless, it has never been demonstrated in the fossil record across a wide range of taxa. Here, by reviewing in-depth the available data of the Late Palaeozoic (~ 350–250 million years ago), I reconstructed an early Pangaean junction-disjunction palaeogeographic model and showed that it coincides strongly with time-calibrated cladograms of the Late Palaeozoic synapsids (the primitive ancestors of modern mammals). The temporal development of the vicariant topology seems to fit closely with the emergence rhythm of the recovered synapsid taxa, suggesting vicariance due to Pangaean separation as the cause of early amniote evolution. The inferred vicariant topology also accounts for the observed pattern in the North American marine biostratigraphic units. Accordingly, the model demonstrates the link between the evolution of life on Earth and palaeogeographic evolution and strongly supports allopatric speciation through vicariance as the prominent mode of amniote evolution. Furthermore, correlations between state-of-the-art biochronostratigraphic charts and this palaeogeographic model suggest that the arido-eustasy model can explain the mid-Permian biotic extinction event and depositional cycles, such as the pre-Zechstein of the Central European Basin.
Structural dynamics of CH3NH3+ and PbBr3− in tetragonal and cubic phases of CH3NH3PbBr3 hybrid perovskite by nuclear magnetic resonance
Authors: Lim Ae Ran , Kim Sun Ha , Joo Yong Lak 
Published: August 2020

AbstractUnderstanding the structural dynamics of lead-halide perovskites is essential for their advanced use as photovoltaics.
Here, the structural dynamics of the CH3NH3 cation and PbBr6 octahedra in the perovskite CH3NH3PbBr3 were studied via nuclear magnetic resonance (NMR) to determine the mechanism of the transition from the tetragonal to cubic phase. The chemical shifts were obtained by 1H, 13C, and 207Pb magic angle spinning NMR and 14N static NMR. The chemical shifts of the 1H nuclei in CH3 and NH3 remained constant with increasing temperature, whereas those of the 13C and 207Pb nuclei varied near the phase transition temperature (TC = 236 K), indicating that the structural environments of 13C and 207Pb change near TC. The spin–lattice relaxation time T1ρ values for 1H, 13C, and 207Pb nuclei increased with increasing temperature and did not exhibit an abrupt change near TC. In addition, the two lines in the 14N NMR spectra superposed into one line near TC, indicating the occurrence of a phase transition to a cubic phase with higher symmetry than tetragonal. Consequently, the main factor causing the phase transition from the tetragonal to cubic phase near TC is a change in the surroundings of the 207Pb nuclei in the PbBr6 octahedra and of the C–N groups in the CH3NH3 cations.
TLR4 896A/G and TLR9 1174G/A polymorphisms are associated with the risk of infectious mononucleosis
Authors: Jabłońska Agnieszka , Studzińska Mirosława , Szenborn Leszek , Wiśniewska-Ligier Małgorzata , Karlikowska-Skwarnik Monika , Gęsicki Tomasz , Paradowska Edyta 
Published: August 2020

AbstractToll-like receptors (TLRs) recognize pathogen-associated molecular patterns and activate innate and adaptive immune responses. Single nucleotide polymorphisms (SNPs) within the TLR genes may influence host–pathogen interactions and can have an impact on the progression of infectious diseases. The present study aimed to investigate the genotype distribution of TLR2 (2029C/T, rs121917864; 2258G/A, rs5743708), TLR4 (896A/G, rs4986790), and TLR9 (− 1237T/C, rs5743836; − 1486T/C, rs187084; 1174G/A, rs352139; and 2848C/T, rs352140) polymorphisms in 149 children and adolescents with infectious mononucleosis (IM) and 140 healthy individuals. The potential association of TLR SNPs with the clinical manifestations of EBV infection was also studied. The presence of TLR2, TLR4, and TLR9 SNPs was identified by polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP). EBV DNA loads were detected by quantitative real-time PCR assay. The TLR4 896 GG and the TLR9 1174 GA genotypes were associated with an increased risk of EBV-related IM in examined patients (p = 0.014 and p = 0.001, respectively). The heterozygous genotype of the TLR4 896A/G SNP was associated with an increased risk of elevated liver enzyme levels and leukocytosis (p < 0.05). Our preliminary study revealed that the TLR4 896A/G and the TLR9 1174G/A polymorphisms seem to be related to the course of acute EBV infection in children and adolescents.