bluuebunny/crossref_metadata_2025_split huggingface dataset:
A data privacy protection scheme for Internet of things based on blockchain
Authors: Gong Jing , Mei Yurong , Xiang Feng , Hong Hanshu , Sun Yibo , Sun Zhixin 
Published: July 2020

AbstractIn recent years, the Internet of things (IoT) equipment has grown rapidly, and the scale of the IoT has also expanded. The IoT is deployed in the system in a centralized manner. At the same time that massive data has put a certain amount of pressure on the storage, the open network environment has not fully protected the privacy of the IoT data, which has become one of the important factors restricting the development of the IoT. Blockchain is a point‐to‐point distributed ledger technology based on cryptographic algorithms. The characteristics of decentralization, tamper resistance, anonymity, and public verifiability can alleviate data security issues in IoT. Ring signature and proxy reencryption are common encryption technologies in the field of privacy protection. Therefore, this article combines blockchain technology with ring signature and proxy reencryption to propose a privacy protection solution for the IoT. Through this solution, the data authorized for sharing in the IoT is transmitted in the system in the form of ciphertext, the identity information of the data sender is protected, and the distributed ledger eases the pressure of mass data storage on a centralized server. The correctness and safety of the proposed scheme are also analyzed.
Introduction: Psychotherapy through the lens of cinema
Authors: Geller Jesse D. 
Published: July 2020

AbstractThe primary aim of this issue of the Journal of Clinical Psychology: In Session is to demonstrate the myriad ways in which movies, their viewing and discussion, can be used to serve therapeutic and educational purposes. It collects together eight essays, an empirical study, and a film script that offer shifting perspectives on questions that are of interest to all therapists, irrespective of their theoretical persuasions: What can be learned about the transformative powers of psychotherapy by studying how filmmakers inspire deeply felt and memorable experiences in their audiences? What are the beneficial and detrimental effects of the ways in which psychotherapists and their patients have been portrayed in films and TV programs? What psychological functions can be served by recommending that a patient watch a particular film? When is it advisable to inquire about the characters in films who have served as the role models that played an important role in the formation of a patient's sense of self? Can films that portray what is best about human beings inspire life‐changing forms of imitative learning? What roles can the viewing and discussion of films play in the education of therapists?
Excavating SARS‐coronavirus 2 genome for epitope‐based subunit vaccine synthesis using immunoinformatics approach
Authors: Chauhan Varun , Rungta Tripti , Rawat Manmeet , Goyal Kapil , Gupta Yash , Singh Mini P. 
Published: July 2020

AbstractSince the outbreak of severe acute respiratory syndrome‐coronavirus 2 (SARS‐CoV‐2) in December 2019 in China, there has been an upsurge in the number of deaths and infected individuals throughout the world, thereby leading to the World Health Organization declaration of a pandemic. Since no specific therapy is currently available for the same, the present study was aimed to explore the SARS‐CoV‐2 genome for the identification of immunogenic regions using immunoinformatics approach. A series of computational tools were applied in a systematic way to identify the epitopes that could be utilized in vaccine development. The screened‐out epitopes were passed through several immune filters, such as promiscuousity, conservancy, antigenicity, nonallergenicity, population coverage, nonhomologous to human proteins, and affinity with human leukocyte antigen alleles, to screen out the best possible ones. Further, a construct comprising 11 CD4, 12 CD8, 3 B cell, and 3 interferon‐γ epitopes, along with an adjuvant β‐defensin, was designed in silico, resulting in the formation of a multiepitope vaccine. The in silico immune simulation and population coverage analysis of the vaccine sequence showed its capacity to elicit cellular, humoral, and innate immune cells and to cover up a worldwide population of more than 97%. Further, the interaction analysis of the vaccine construct with Toll‐like receptor 3 (immune receptor) was carried out by docking and dynamics simulations, revealing high affinity, constancy, and pliability between the two. The overall findings suggest that the vaccine may be highly effective, and is therefore required to be tested in the lab settings to evaluate its efficacy.
Maternal separation affects the electrophysiological properties of Aδ‐fibres and nociceptive behaviours in male and female mice
Authors: Paniagua Nancy , Girón Rocío , Goicoechea Carlos , Martín‐Fontelles Mª Isabel , Bagues Ana 
Published: July 2020

AbstractAimEarly life adverse effects have been associated with an increased risk of suffering pain syndromes in adulthood. Although animal models are of great importance to study modifications of pain sensitivity, up to date the results obtained are contradicting due to the varied methodologies used. Therefore, the aim of the present study was to characterise, as a whole, possible modifications in visceral and somatic nociceptive responses in male and female ICR mice, submitted to two different protocols of maternal separation (MS), and possible modifications in the electrophysiological properties of peripheral nociceptive Aδ‐primary afferents.Main methodsMale and female mice were submitted to 3 or 4–8 hr of daily MS from postnatal day (PND) 2–17 and early weaned. On PND 67 von Frey, hot plate and writhing tests were performed. Afterwards electrophysiological recordings were carried out, using the in vitro skin‐saphenous nerve preparation in males.Key findingsThe short separation protocol of MS did not modify nociceptive sensitivity; but when mice were separated from their dams for the long separation, mechanical pain thresholds were modified in male and female mice and visceral nociception was increased in female mice. Electrophysiological recordings showed that cutaneous Aδ‐fibres were sensitised and their mechanotransduction properties were altered in both MS protocols.SignificanceAlthough MS increases the activity and the mechanosensitivity of cutaneous Aδ‐afferent fibres at both short and long periods of separation, only the longer interval of time induces nociceptive sensitivity alterations during adulthood. These results highlight the possible influence of a stress free environment during childhood to reduce nociceptive alterations in adulthood.
Comparative foliar anatomical and pollen morphological studies of Acanthaceae using light microscope and scanning electron microscope for effective microteaching in community
Authors: Raza Jamil , Ahmad Mushtaq , Zafar Muhammad , Athar Mohammad , Sultana Shazia , Majeed Salman , Yaseen Ghulam , Imran Muhammad , Nazish Moona , Hussain Amir 
Published: July 2020

AbstractIn this study, foliar anatomy and pollen morphology of 10 species of Acanthaceae has been investigated using light and scanning electron microscopy. The study was aimed to highlight the role of microscopy in microteaching at community for proper characterization of plants using palyno‐anatomical characters including pollen type, exine sculpturing, shape of epidermal cells, pattern of anticlinal wall, type and size of stomata, and trichome. Most of the species have polygonal cell shapes but some species have irregular, tetragonal, and pentagonal shape of epidermal cells. The largest epidermal cell length on adaxial and abaxial surface were observed in Asystasia gangetica 66.95 and 87.40 μm whereas least was observed on adaxial surface in Justicia adhatoda 36.9 μm and on abaxial surface in Barleria cristata 35.65 μm. In anatomy, species have diacytic type of stomata, whereas stomata of paracytic type observed in two species, while in A. gangetica cyclocytic type of stomata are present. Quantitively on abaxial surface, largest stomata length 29.9 μm and width 24.30 μm was noted in B. cristata. While shortest stomata length was observed in Ruellia prostrata 25.95 μm whereas minimum width of stomata was examined in Barleria acanthoides 2.05 μm. The diversity of trichomes are present in all species except in Ruellia brittoniana. Acanthaceae can be characterized by exhibiting different pollen morphology having five types of pollen shapes, prolate, spheroidal, perprolate, subprolate, and oblate spheroidal. Exine peculiarities showing variations such as reticulate, granulate, coarsely reticulate, lophoreticulate, perforate tectate, and granulate surface were examined.
A thematic analysis of health information technology use among cancer genetic counselors
Authors: Allen Caitlin G. , Ritchie Jordon B. , Morrison Heath , Lauzon Steven D. , Nichols Michelle , Schiffman Joshua D. , Hughes Halbert Chanita , Welch Brandon M. 
Published: July 2020

AbstractAs precision medicine becomes a mainstay in health care, the use of health information technology (IT) platforms will play an important role in the delivery of services across the cancer care continuum. Currently, there is both limited understanding about perceptions of health IT tools and barriers to their use among cancer genetic counselors. We assessed open‐ended responses from a survey conducted among 128 board‐certified cancer genetic counselors in the United States. We evaluated the utility of ten health IT tools and perceived barriers to adoption. Responses about characteristics of health IT tools that influence current use (i.e., technology‐specific challenges) were deductively analyzed using the diffusion of innovations (DOI) characteristics. Responses about cancer genetic counselors' perceived challenges to adopting health IT tools (i.e., discipline‐specific challenges) were inductively coded using a thematic approach. DOI innovation characteristics included mixed perceptions about the relative advantage, complexity, compatibility, trialability, and observability of tools based on the type of tool and perceived end‐user. One‐third of participants indicated that they were considering adopting or switching health IT tools. Common barriers to adoption included no perceived need for change, lack of organizational infrastructure, cost, and lack of decision‐making power. Our findings indicate that addressing barriers to use and adoption of health IT may allow for expansion of these tools among cancer genetic counselors. Integrating health IT is critical for enhancing cancer genetic counselors’ capacity to address patient needs and realizing the potential of precision medicine.
Clinical exacerbation of SARS‐CoV2 infection after fingolimod withdrawal
Authors: Gomez‐Mayordomo Victor , Montero‐Escribano Paloma , Matías‐Guiu Jordi A. , González‐García Nuria , Porta‐Etessam Jesús , Matías‐Guiu Jorge 
Published: July 2020

AbstractThe role of disease‐modifying therapies in patients with autoimmune disorders during severe acute respiratory syndrome coronavirus 2 (SARS‐CoV2) infection is controversial. Immunocompromised patients could have a more severe coronavirus disease‐2019 (COVID‐19) due to the absence of an adequate immune response against the SARS‐CoV‐2. However, therapies that act on immune response could play a protective role by dampening the cytokine‐release syndrome. Fingolimod is a drug used for immune therapy in patients with multiple sclerosis (MS) through the sequestration of activated lymphocytes in the lymph nodes. We report the case of a 57‐year‐old man with relapsing‐remitting MS treated with fingolimod that showed a reactivation of COVID‐19 with signs of hyperinflammation syndrome after fingolimod withdrawal. Our case suggests that discontinuation of fingolimod during COVID‐19 could imply a worsening of SARS‐CoV2 infection.
Early use of tocilizumab in the prevention of adult respiratory failure in SARS‐CoV‐2 infections and the utilization of interleukin‐6 levels in the management
Authors: Antony Suresh J. , Davis Michelle A. , Davis Monique G. , Almaghlouth Nouf K. , Guevara Roberto , Omar Fahad , Del Rey Fernando , Hassan Ali , Arian Muhammad U. , Antony Nishaal , Prakash Bharat V. 
Published: July 2020

AbstractRespiratory failure in severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) infection appears related to cytokine release syndrome that often results in mechanical ventilation (MV). We investigated the role of tocilizumab (TCZ) on interleukin‐6 (IL‐6) trends and MV in patients with SARS‐CoV‐2. In this longitudinal observational study, 112 patients were evaluated from 1 February to 31 May 2020. TCZ was administered followed by methylprednisolone to patients with >3L oxygen requirement and pneumonia severity index score ≤130 with computed tomography scan changes. IL‐6, C‐reactive protein (CRP), ferritin, lactate dehydrogenase (LDH), D‐dimer, and procalcitonin were monitored on days 0, 3, and 6 of therapy. Statistical analyses were performed with significance ≤0.05. Eighty out of 112 SARS‐CoV‐2‐positive patients (45 males, 56.96%; 34 females, 43.04%) were included in this study. Seven patients expired (8.75%) and nine patients required MV (11.25%). Median IL‐6 levels pre‐administration of TCZ was 342.50 (78.25‐666.25) pg/mL compared with post‐administration on day 3 (563; 162‐783) pg/mL (P < .00001). On day 6, the median dropped to 545 (333.50‐678.50) pg/mL compared with day 3 (P = .709). CRP, ferritin, LDH, and D‐dimer levels were reduced after TCZ therapy. Early use of TCZ may reduce the need for MV and decrease CRP, ferritin, LDH, and D‐dimer levels. The sequential use of methylprednisolone for 72 hours seems to potentiate the effect and prolong the suppression of the cytokine storm. IL‐6 levels may be helpful as a prognostic tool.
Determination of levetiracetam in human plasma by online heart‐cutting liquid chromatography: Application to therapeutic drug monitoring
Authors: Önal Cem , Kul Aykut , Ozdemir Murat , Sagirli Olcay 
Published: July 2020

AbstractLevetiracetam is an antiepileptic drug for the treatment of psychiatric patients. In this study, a selective, straightforward, and rapid online heart‐cutting liquid chromatography method was developed for the therapeutic drug monitoring of levetiracetam. This method allows for the determination of levetiracetam in human plasma without complex sample preparation. The mobile phases consisted of 30 mM aq. orthophosphoric acid solution/methanol (70:30) at a flow rate of 1 mL/min for the first system and 10 mM aq. orthophosphoric acid solution/methanol (55:45) at a flow rate of 1 mL/min for the second system. The first separation was carried out on a GL Sciences Intersil ODS‐3 column (4.6 mm × 150 mm, 3 µm) and the second separation was carried out on a Restek Ultra PFPP column (4.6 mm × 150 mm, 5 µm). The detection was carried out at 205 nm for both systems. The method was validated for selectivity and linearity, which were in the 6–60 µg/mL range. Intra‐ and interassay accuracies were <112.6%, and the intra‐ and interassay precisions were <6.4% for all quality control samples. The lower limit of quantitation was 6 µg/mL. The developed method was successfully applied for therapeutic drug monitoring of plasma samples from patients.
Cure or curd: Modification of lipid profiles and cardio‐cerebrovascular events after hepatitis C virus eradication
Authors: Huang Chung‐Feng , Dai Chia‐Yen , Yeh Ming‐Lun , Huang Ching‐I , Lee Hsiang‐Chun , Lai Wen‐Ter , Liang Po‐Cheng , Lin Yi‐Hung , Hsieh Ming‐Yen , Hou Nai‐Jen , Lin Zu‐Yau , Chen Shinn‐Cherng , Huang Jee‐Fu , Chuang Wan‐Long , Yu Ming‐Lung 
Published: July 2020

AbstractHepatitis C virus (HCV) eradication deteriorates lipid profiles. Although HCV eradication may reduce the risk of vascular events as a whole, whether deteriorated lipid profiles increases the risk of cardio‐cerebral disease in certain patients is elusive. Serial lipid profiles were measured before, during, at and 3 months after the end of direct‐acting antivirals (DAAs) therapy, and annually thereafter in chronic hepatitis C patients who achieved a sustained virological response (SVR, undetectable HCV RNA at posttreatment week 12). The primary end‐point was the occurrence of the events. A total of 617 patients were included, with a mean follow‐up period of 26.8 months (range: 1‐65 months). The total cholesterol and low‐density lipoprotein cholesterol (LDL‐C) levels increased significantly from treatment week 4 to 2 years after treatment. Logistic regression analysis revealed that the factors independently associated with a significant cholesterol increase included age (odds ratio [OR]/95% confidence intervals [CIs]:1.02/1.006‐1.039, P = .007) and smoking (OR/CI:3.21/1.14‐9.02, P = .027). Five patients developed cardio‐cerebral diseases during 1376 person‐years follow‐up period. Compared to patients without vascular events, a significantly higher proportion of those with vascular events experienced an LDL‐C surge >40% (80% vs 19.9%, P = .001). Cox‐regression analysis revealed that an LDL‐C surge >40% was the only factor predictive of vascular events (HR/CI: 15.44/1.73‐138.20, P = .014). Dyslipidemia occurred after HCV eradication, and it was associated with the risk of cardio‐cerebrovascular diseases. Attention should also be paid to the extrahepatic consequence beyond liver‐related complications in the post‐SVR era.
Dopamine D1 Receptors Regulate Spines in Striatal Direct‐Pathway and Indirect‐Pathway Neurons
Authors: Suarez Luz M. , Solis Oscar , Sanz‐Magro Adrian , Alberquilla Samuel , Moratalla Rosario 
Published: July 2020

AbstractBackgroundDopamine transmission is involved in the maintenance of the structural plasticity of direct‐pathway and indirect‐pathway striatal projection neurons (d‐SPNs and i‐SPNs, respectively). The lack of dopamine in Parkinson's disease produces synaptic remodeling in both types of SPNs, reducing the length of the dendritic arbor and spine density and increasing the intrinsic excitability. Meanwhile, the elevation of dopamine levels by levodopa recovers these alterations selectively in i‐SPNs. However, little is known about the specific role of the D1 receptor (D1R) in these alterations.MethodsTo explore the specific role of D1R in the synaptic remodeling of SPNs, we used knockout D1R mice (D1R−/−) and wild‐type mice crossed with drd2‐enhanced green fluorescent protein (eGFP) to identify d‐SPNs and i‐SPNs. Corticostriatal slices were used for reconstruction of the dendritic arbors after Lucifer yellow intracellular injection and for whole‐cell recordings in naïve and parkinsonian mice treated with saline or levodopa.ResultsThe genetic inactivation of D1R reduces the length of the dendritic tree and the spine density in all SPNs, although more so in d‐SPNs, which also increases their spiking. In parkinsonian D1R−/− mice, the spine density decreases in i‐SPNs, and this spine loss recovers after chronic levodopa.ConclusionsD1R is essential for the maintenance of spine plasticity in d‐SPNs but also affects i‐SPNs, indicating an important crosstalk between these 2 types of neurons. © 2020 International Parkinson and Movement Disorder Society
Perinatal exposure to maternal obesity: Lasting cardiometabolic impact on offspring
Authors: Kislal Sezen , Shook Lydia L. , Edlow Andrea G. 
Published: July 2020

AbstractEvidence from epidemiological, clinical, and animal model studies clearly demonstrates that prenatal and lactational maternal obesity and high‐fat diet consumption are associated with cardiometabolic morbidity in offspring. Fetal and offspring sex may be an important effect modifier. Adverse offspring cardiometabolic outcomes observed in the setting of maternal obesity include an increased risk for obesity, features of metabolic syndrome (hypertension, hyperglycemia and insulin resistance, hyperlipidemia, increased adiposity), and non‐alcoholic fatty liver disease. This review article synthesizes human and animal data linking maternal obesity and high‐fat diet consumption in pregnancy and lactation to adverse cardiometabolic outcomes in offspring. We review key mechanisms underlying skeletal muscle, adipose tissue, pancreatic, liver, and central brain reward programming in obesity‐exposed offspring, and how such malprogramming contributes to offspring cardiometabolic morbidity.
A Deep Learning‐Based Tumor Classifier Directly Using MS Raw Data
Authors: Dong Hao , Liu Yi , Zeng Wen‐Feng , Shu Kunxian , Zhu Yunping , Chang Cheng 
Published: July 2020

AbstractSince the launch of Chinese Human Proteome Project (CNHPP) and Clinical Proteomic Tumor Analysis Consortium (CPTAC), large‐scale mass spectrometry (MS) based proteomic profiling of different kinds of human tumor samples have provided huge amount of valuable data for both basic and clinical researchers. Accurate prediction for tumor and non‐tumor samples, as well as the tumor types has become a key step for biological and medical research, such as biomarker discovery, diagnosis, and monitoring of diseases. The traditional MS‐based classification strategy mainly depends on the identification and quantification results of MS data, which has some inherent limitations, such as the low identification rate of MS data. Here, a deep learning‐based tumor classifier directly using MS raw data is proposed, which is independent of the identification and quantification results of MS data. The potential precursors with intensities and retention times from MS data as input is first detected and extracted. Then, a deep learning‐based classifier is trained, which can accurately distinguish between the tumor and non‐tumor samples. Finally, it is demonstrated the deep learning‐based classifier has a good performance compared with other machine learning methods and may help researchers find the potential biomarkers which are likely to be missed by the traditional strategy.
Spectroscopic, X‐ray Crystal, DFT and In Vitro Analysis of 3‐(2,4‐Dimethoxy‐3‐(3‐methylbut‐2‐en‐1‐yl)phenyl) Acrylic Acid
Authors: Rather Zubaid‐ul‐khazir , Banday Javid A. , Chisti Hamida‐Tun‐Nisa 
Published: July 2020

Abstract7‐Methoxy‐8‐(3‐methylbut‐2‐enyl)‐2‐chromenone (osthol) (OL) was modified to its acid derivative; 3‐(2,4‐Dimethoxy‐3‐(3‐methylbut‐2‐en‐1‐yl)phenyl)acrylic acid (OAD) via base catalysed ring opening mechanism. The structural properties, electronic spectra, single crystal analysis, with the support of density functional theory (DFT), have been investigated extensively. Furthermore, in vitro cell viability was carried out on different cancer cell lines.
Mechanochemical and Conventional Synthesis of Copper(II) Coordination Polymers Bearing Arylhydrazone of Acetoacetanilide and Their Catalytic Activity in Conversion of Acetone to Acetic Acid
Authors: Gurbanov Atash V. , Martins Luísa M. D. R. S. , Kopylovich Maximilian N. , Sutradhar Manas , Zubkov Fedor I. , Mahmudov Kamran T. , Pombeiro Armando J. L. 
Published: July 2020

AbstractCopper(II) coordination polymers, [Cu(μ2‐HL)]n⋅n(CH3)2C=O (1), [Cu(μ1‐HL)(CH3OH)]n (2) and [Cu(μ1‐HL)(H2O)]n (3) were isolated by recrystallization (in acetone, methanol and dimethylformamide, respectively) of an intermediate produced by a solid state ball milling reaction of CuCl2⋅2H2O with 2‐(2‐(1,3‐dioxo‐1‐(phenylamino)butan‐2‐ylidene)hydrazinyl)benzoic acid (H3L). Reaction under conventional fully solution‐based conditions of CuCl2⋅2H2O with H3L in acetone and dimethylformamide leads to 3, and in methanol to the known monomeric compound [Cu(HL1)(H2O)(CH3OH)] (4). All the coordination polymers 1−3 act as homogenous catalysts for the peroxidative (H2O2) oxidation of acetone to acetic acid with yields up to 50% and at room temperature.