bluuebunny/crossref_metadata_2025_split huggingface dataset:
Abkehr von der Depressionspraxis
Authors: Steiger-Sackmann Sabine 
Published: July 2020

Mit seinem Urteil vom 30. November 2017 kehrt das Bundesgericht von seiner Praxis ab, bei Versicherten mit leicht- bis mittelgradigen Depressionen zusätzlich zu den Leistungsvoraussetzungen für eine IV-Rente zu verlangen, dass eine Therapieresistenz vorliegt. Diese Änderung der Rechtsprechung ist zu begrüssen, wenn auch einzelne Ausführungen der Urteilsbegründung Anlass zu kritischen Einwänden geben.
Staphylococcus aureus and methicillin-resistant Staphylococcus aureus (MRSA) in drinking water fountains in urban parks
Authors: Santos Geyse A. C. , Dropa Milena , Rocha Solange M. , Peternella Francisca A. S. , Razzolini Maria Tereza Pepe 
Published: July 2020

AbstractThe presence of Staphylococcus aureus in drinking water is a concern because of its potential to cause human infection and also because of its multiple antimicrobial resistance. This study evaluated the water quality of drinking water fountains and mist makers in four municipal parks of São Paulo for 13 months. Although all samples met bacteriological water quality criteria according to Brazilian regulations, the absence of residual chlorine (&lt;0.1 mg/L) was observed. These data were significantly correlated with the frequency of S. aureus that was found in 25.2% of the samples. The mecA gene was detected in 36.7% of the isolates demonstrating its potential for resistance to several antimicrobials. Furthermore, 27.3% isolates carrying the mecA gene had methicillin-resistant Staphylococcus aureus (MRSA) phenotypic potential. The presence of S. aureus with characteristics of microbial resistance in water for human consumption is an unprecedented finding. Hence, conducting surveillance for opportunistic bacteria, such as staphylococci in drinking water, is reasonable to take control measures and to protect human health, especially in public places with high attendance.
Optimal allocation model of the water resources in Harbin under representative concentration pathway scenarios
Authors: Meng Fanxiang , Li Linqi , Li Tianxiao , Fu Qiang 
Published: July 2020

AbstractWater conservation is a strategic choice for sustainable societal development. Rational planning and allocation of water resources is an effective way to improve the efficiency of water resource utilization. Taking Harbin as an example, this paper constructs a linear fractional programming model based on chance-constrained programming. The model can reflect the randomness of water inflow under different climatic conditions while taking into account the interests of different decision makers at the upper and lower levels and the two contradictory objectives of maximizing economic benefits and minimizing water demands to improve water distribution efficiency and reduce the complexity of water resource distribution systems. The results showed that under the three climatic scenarios of RCP4.5, RCP6.0, and RCP8.5, the agricultural water supply accounted for 90.04%, 87.66%, and 84.15% of the total regional water supply, respectively. In the process of building the model, considering the importance of sewage treatment in water safety evaluations, the cost of sewage treatment is included in the upper-level benefits of the model. The sustainable development of water resources should be guaranteed while rationally allocating water resources and pursuing economic benefits.
Integrative Model of Building the Education System as a Tool of Providing Well-Balanced Transforming Labour Market
Authors: Kuzminov A. N. , Tyaglov S. G. , Arhipova L. S. , Sedova N. V. 
Published: July 2020

The article provides a new approach to building a system of continuous education as interdisciplinary synthesis of theoretical, methodological and instrumental tools of analyzing, forecasting, projecting and state governance. These problems are discussed in the context of labour market stability. The research object is the processes of developing the fundamental institutions of labour market in their interconnection with education and economic systems of mezzo-level, whose complication could hinder the use of classical instrumental means of analysis and promote the attraction of new models, including those based on cognitive and cenology approaches. The key lines in academic research in the field are being studied with special focus on approaches to shaping a new institutional model of the education system. To attain the set goal tools are used, which take into account complexity of all sub-systems being investigated, including versatile nature of processes taking place on labour market and their interconnection, which leads to impossibility to single out and research in detail separate phenomena. The theoretical and methodological approach to forecast and governance of well-balanced labour market at the expense of coordination between key stakeholders’ interests and principle institutions relies on laws of imitation and cenology modeling, which can ensure realization of strategic goals of sustainable development through building the optimum supply within the frames of continuous education.
Optimization, Purification and Antitumor Activity of Kodamaea ohmeri ANOMY L-Asparaginase Isolated from Banana Peel
Authors: Shabana Ahmed M.I. , Shetaia Yousseria M. , Abdelwahed Nayera A.M. , Esawy Mona A. , Alfarouk Omar R. 
Published: July 2020

Objective:
L-Asparaginase is an important enzyme that converts L-asparagine to L-aspartate
and ammonia. Microbial L-asparaginase has important applications as anticancer and food processing
agents.

Methods:
This study reported the isolation, screening of a local yeast isolate from banana peel for
L-asparaginase production using submerged fermentation, optimization of the production, purification,
and anticancer assay of L-asparaginase. The yeast isolate was identified as Kodamaea ohmeri ANOMY
based on the analysis of nuclear large subunit (26S) rDNA partial sequences. It was a promising
L-asparaginase producer with a specific activity of 3059±193 U/mg in a non-optimized medium. The
classical one-variable-at-a-time method was used to optimize the production medium components, and
it was found that the elimination of K2HPO4 from the medium increased L-asparaginase specific activity
(3100.90±180 U/mg).

Results:
Statistical optimization of L-asparaginase production was done using Plackett-Burman and
Box-Behnken designs. The production medium for the maximum L-asparaginase specific activity
(8500±578U/mg) was as follows (g/L): L-asparagine (7.50), NaNO3 (0.50), MgSO4.7H2O (0.80), KCl
(0.80) associated with an incubation period of 5 days, inoculum size of 5.60 %, and pH (7.0). The optimization
process increased L-asparaginase production by 2.78-fold compared to the non-optimized
medium. L-Asparaginase was purified using ammonium sulphate precipitation followed by gel filtration
on a Sephadex G-100 column. Its molecular weight was 66 KDa by SDS-PAGE analysis.

Conclusion:
The cell morphology technique was used to evaluate the anticancer activity of
L-asparaginase against three different cell lines. L-Asparaginase inhibited the growth of HepG-2,
MCF-7, and HCT-116 cells at a concentration of 20, 50, and 60 μL, respectively.
Glycyrrhetinic Acid and TAT Peptide Modified Dual-functional Liposomes for Treatment of Hepatocellular Cancer
Authors: Huang Sixi , Ren Di , Wu Xinrong , Li Ming , Yu Xuesong , Nie Xiaoling , Wang Ying , Wang Yan 
Published: July 2020

Background:
Surgery remains the front-line therapeutic strategy to treat early hepatocellular
carcinoma (HCC). However, the 5-year recurrence rates of HCC patients are high. 10-
Hydroxycamptothecin (10-HCPT) is a known anti-HCC agent but its poor solubility and bioavailability
have limited its clinical use.

Objective:
In this study, we developed a novel nanoliposome encapsulated 10-hydroxycamptothecin
modified with glycyrrhetinic acid (GA) and TAT peptide (GA/TAT-HCPT-LP) for the treatment of
HCC. Dual modified GA and TAT can enhance tumor targeting and tumor penetration.

Methods:
The GA/TAT-HCPT-LP NPs were synthesized using the thin-film dispersion method.
GA/TAT-HCPT-LP were characterized for particle size, zeta potential and morphology. Drug release
from the GA/TAT-HCPT-LP liposomes was measured by dialysis. Cell-uptake was assessed by microscopy
and flow cytometry. Cell proliferation, migration and apoptosis were measured to evaluate in vitro
antitumor activity of GA/TAT-HCPT-LP via CCK-8 assays, Transwell assays, and flow cytometry, respectively.
The in vivo distribution of GA/TAT-HCPT-LP was evaluated in HCC animal models. Tumor-
bearing mouse models were used to assess the in vivo therapeutic efficacy of GA/TAT-HCPT-LP.

Results:
The mean particle size and mean zeta potential of GA/TAT-HCPT-LP were 135.55 ± 2.76 nm
and -4.57 ± 0.23 mV, respectively. Transmission electron micrographs (TEM) showed that the
GA/TAT-HCPT-LP had a near spherical shape and a double-membrane structure. GA/TAT-HCPT-LP
led to slow and continuous drug release, and could bind to HepG2 cells more readily than other groups.
Compared to control groups, treatment with GA/TAT-HCPT-LP had a significantly large effect on inhibiting
cell proliferation, tumor cell migration and cell apoptosis. In vivo assays showed that GA/TATHCPT-
LP selectively accumulated in tumor tissue with obvious antitumor efficacy.

Conclusions:
In conclusion, the synthesized GA/TAT-HCPT-LP could effectively target tumor cells and
enhance cell penetration, highlighting its potential for hepatocellular cancer therapy.
Heck Cross-Coupling Reactions in Ionic Liquid mediated Pd Nanocatalysts
Authors: Gautam Prashant , Srivastava Vivek 
Published: July 2020

:
We straightforwardly synthesized 18 different types of palladium nanoparticles by using a
series of palladium metal precursors and ionic liquids. All the materials went for XRD, TEM, and ICPOES
analysis, before going to Heck cross-coupling reaction as a catalyst. We evaluated the catalytic
performance of our developed IL#Pd MNP catalyst over Heck cross-coupling reaction between different
terminal olefins with various 3-iodo-benzopyrones, including sterically hindered, electron-rich,
electron neutral and electron-deficient systems. We obtained the Heck cross-coupling reaction product
in good to average yield under phosphine free reaction condition with an added advantage of 6 times
catalyst recycling.
Neuroprotective Potential of Adenyl Cyclase/cAMP/CREB and Mitochondrial CoQ10 Activator in Amyotrophic Lateral Sclerosis Rats
Authors: Alam Mohmmad M. , Minj Elijabeth , Yadav Rajeshwar K. , Mehan Sidharth 
Published: July 2020

Aim:
To investigate neuroprotective potential of Forskolin (FSK) in combination with
Solanesol (SNL) along with clinically proven drugs (riluzole, baclofen, and citalopram) on behavioral,
molecular and neurochemical alterations in methyl mercury-induced Amyotrophic Lateral Sclerosis
(ALS) rats.

Background:
ALS is a motor neuron disease in which oxidative stress is the principal mechanism
of neuronal death which can be mimicked by the dominant mutations in an antioxidant enzyme
SOD-1. Due to MeHg neurotoxicity, behavioral and neurochemical alterations occur in rats. During
ALS, mitochondrial CoQ10 dysfunctioning and downregulation of adenyl cyclase/CREB are
major pathological hallmark for neurodegeneration in ALS. Clinically proven drug therapy comes
with limited therapeutic involvement and is used as approachable therapy in ALS patients.

Objective:
Therefore, current research explores the up-regulation of adenyl cyclase/cAMP/CREB
by FSK 30, 60 mg/kg in combination with mitochondrial ETC-coenzyme-Q10 precursor SNL 15,
30 mg/kg can be a preventive therapeutic approach to overcome the ALS like symptoms.

Methods:
MeHg (5 mg/kg) is a neurotoxic compound that leads to ALS like behavioral & neurochemical
alterations.

Results:
Chronic treatment with the combination of FSK 30,60 mg/kg and SNL 15,30 mg/kg alone
and along with standard drugs citalopram (5 mg/kg), riluzole (5 mg/kg) and baclofen (3 mg/kg) increased
the adenyl cyclase and mitochondrial CoQ10 and ETC-complexes enzyme levels and
shows the neuroprotective potential by significantly improving the cognitive deficitslocomotion,
grip strength, and restoration of neurochemicals alterations along with reducing the level of inflammatory
mediators and oxidative stress in ALS rats.

Conclusion:
Thus, we concluded that FSK in combination with SNL along with standard drugs
can be a possible therapeutic approach for the treatment of ALS.
A New 1,2-Benzisoxazolin-3-one Synthetized from Eugenol Shows Anti- Candida Spp. Activity, Specially Against Opportunistic Candida Glabrata
Authors: Fregnan Antonio M. , Júnior Antonio A. Fernandes , Alvarenga Dalila J. , Oliver Josidel C. , Silva Naiara C. , Coelho Luiz F.L. , Dias Amanda L. Tranches , Dias Danielle F. , de Souza Thiago B. , Carvalho Diogo T. 
Published: July 2020

Background:
In view of the need of discovering new antifungal agents, the synthesis of
compounds comprising new structural scaffolds is an important approach, since they may act by
new mechanisms of action and overcome resistance issues.

Objective:
Synthesis of new 1,2-benzisoxazolin-3-ones inspired in eugenol and ebselen as new antifungal
agents against Candida spp.

Methods:
A series of 1,2-benzisoxazolin-3-ones, based on eugenol and analogs, was synthesized
by the cyclization of the respective hydroxamic acids under Mitsunobu conditions. IR, NMR, and
MS spectroscopies characterized the synthesized compounds. The final products were screened for
their antifungal activity against five Candida spp., using microdilution assays and cytotoxicity
against peripheral blood mononuclear cells by the MTT method.

Results:
The products were obtained in good yields and the results showed that most of the synthesized
compounds were better antifungal agents than eugenol against the Candida species evaluated
and that the derivative IVd (IC50 53 μmol L-1) showed activity comparable to fluconazole against
C. glabrata. Moreover, IVd showed the best selectivity profile among the synthesized new compounds.

Conclusion:
A new 1,2-benzisoxazolin-3-one (IVd) was obtained as a promising candidate for the
development of new antifungal agents based on a new chemical scaffold.
Molecular Docking, QSAR and Microscopic Studies of Anti-trypanosomal Compounds from the Pathogen Box
Authors: Ogunleye Adewale J. , Olaolu Olushola S. , Ibrahim Nuraddeen B. , James Anthony A. 
Published: July 2020

Background:
Trypanosoma brucei (T. brucei) is the cause of the deadly human African
trypanosomiasis (HAT) with a case fatality ratio of 10%.

Objective:
Targeting the essential Trypanosomal glucose metabolism pathway through the inhibition
of phosphoglycerate kinase (PGK) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
is a valid strategy for anti-T. brucei drug development.

Methods:
Here, quantitative structure activity relationship, molecular docking and microscopic
studies were used to describe the mode of inhibition of selected compounds from the pathogen box
PGK and GAPDH.

Results:
We identified 4 hit compounds from the pathogen box with optimal binding and
chemical interactions. Notably, it was identified that interacting charge surface and atomic mass
were key aspects of both PGK and GAPDH inhibition. Also, novel anti-trypanosomal compounds
were identified from the pathogen box and their half maximal inhibitory concentrations
were described.

Conclusions:
Our study presents new anti-trypanosomal compounds with optimal pharmacological
profiles and an optimization strategy for improving target specificity in the rational design of novel
anti-trypanosomal compounds.
Quality-by-design Enabled Chitosan Nanoparticles for Antitubercular Therapy: Formulation, Statistical Optimization, and In Vitro Characterization
Authors: Chogale Manasi M. , Gaikwad Sujay S. , Kulkarni Savita P. , Patravale Vandana B. 
Published: July 2020

Background:
Tuberculosis (TB) continues to be among the leading causes of high mortality
among developing countries. Though a seemingly effective treatment regimen against TB is
in place, there has been no significant improvement in the therapeutic rates. This is primarily owing
to the high drug doses, their associated side-effects, and prolonged treatment regimen. Discontinuation
of therapy due to the severe side effects of the drugs results in the progression of the infection
to the more severe drug-resistant TB.

Objectives:
Reformulation of the current existing anti TB drugs into more efficient dosage forms
could be an ideal way out. Nanoformulations have been known to mitigate the side effects of toxic,
high-dose drugs. Hence, the current research work involves the formulation of Isoniazid (INH; a
first-line anti TB molecule) loaded chitosan nanoparticles for pulmonary administration.

Methods:
INH loaded chitosan nanoparticles were prepared by ionic gelation method using an anionic
crosslinker. Drug-excipient compatibility was evaluated using DSC and FT-IR. The formulation
was optimized on the principles of Quality-by-Design using a full factorial design.

Results:
The obtained nanoparticles were spherical in shape having an average size of 620±10.97
nm and zeta potential +16.87±0.79 mV. Solid-state characterization revealed partial encapsulation
and amorphization of INH into the nanoparticulate system. In vitro release study confirmed an extended
release of INH from the system. In vitro cell line-based safety and efficacy studies revealed
satisfactory results.

Conclusion:
The developed nanosystem is thus an efficient approach for antitubercular therapy.
Recent Patents on the Industrial Application of Alpha-amylases
Authors: Ferreira André V. F. , Silva Felipe F. , Silva Ana Amélia M. , Azevedo Lucas S. , da Fonseca Sara T. D. , Camilo Nathalia H. , dos Santos Keiza P. E. , de Carvalho Leiliane C. , Tarabal Vinícius S. , da Silva Jonatas O. , Machado Juliana M. , Nogueira Laís M. , Torres Fernando Araripe G. , Galdino Alexsandro S. 
Published: July 2020

Background:
Alpha-amylases are enzymes capable of degrading polysaccharides,
such as starch and glycogen. Found in various organisms, such as fungi and bacteria,
these enzymes have great biotechnological potential due to their insertion in several
industrial sectors ranging from food to biofuels.

Objective:
The aim of this study was to analyze patents deposited in intellectual property
databases on alpha-amylases in the fields of food, beverages, detergents, animal feeds,
biofuels, pharmaceuticals and textiles, with the search period being 5 years.

Methods:
This study targeted the use of alpha-amylases in various industrial sectors, so
searches were carried out on the intellectual property database Espacenet website (European
Patent Office - EPO) which contains more than 90 million patents deposited in its
database.

Results:
During the search for patents filed in the last 5 years, 186 were found related to
the use of alpha-amylases. These were disturbed as follows: 84 (biofuel), 41 (drinks), 16
(pharmaceuticals), 15 (detergents), 11 (food), 10 (animal feed), 9 (textiles). From the total
number of patents found, we selected 6 from each area, except pharmaceutical products,
to discuss and provide information on the application of this enzyme.

Conclusion:
This study demonstrated that the sectors of beverages and animal feed have
preferences for thermostable alpha-amylases while sectors such as food, biofuels and textiles
only regarded the importance of enzymatic efficiency. The detergent sector presented
the greatest use of alpha-amylases which had distinct biochemical characteristics as solvent
resistance and thermostability. The pharmaceutical sector was the one that presented
less patents related to the application of alpha amylases. In addition, this work showed
that China is the country with the highest patent registration for the use of alpha-amylases
in the analyzed period.
A New Keeper Domino Logic Based Full Adder for High-Speed Arithmetic Circuits
Authors: Bansal Deepika , Nagar Bal Chand , Singh Brahamdeo Prasad , Kumar Ajay 
Published: July 2020

Objective:
A new efficient keeper circuit has been proposed in this article for achieving low
leakage power consumption and to improve the power delay product of the dynamic logic using carbon
nanotube MOSFET.

Methods:
As a benchmark, a one-bit adder has been designed and characterized with both technologies
Si-MOSFET and CN-MOSFET using proposed and existing dynamic circuits. Furthermore, a
comparison has been made to demonstrate the superiority of CN-MOSFET technology with Synopsys
HSPICE tool for multiple bit adders available in the literature.

Result:
The simulation results show that the proposed keeper circuit provides lower static and dynamic
power consumption up to 57 and 40%, respectively, as compared to the domino circuits using
32nm CN-MOSFET technology provided by Stanford University. Moreover, the proposed keeper configuration
provides better performance using Si-MOSFET and CN-MOSFET technologies.

Conclusion:
A comparison of the proposed keeper with previously published designs is also given in
terms of power consumption, delay and power delay product with the improvement up to 75, 18 and
50%, respectively. The proposed circuit uses only two transistors, so it requires less area and gives
high efficiency.
MicroRNA Expression Profiling of Epithelial Ovarian Cancer Identifies New Markers of Tumor Subtype
Authors: Rattanapan Yanisa , Korkiatsakul Veerawat , Kongruang Adcharee , Siriboonpiputtana Teerapong , Rerkamnuaychoke Budsaba , Chareonsirisuthigul Takol 
Published: July 2020

Background:
Epithelial Ovarian Cancer (EOC) is often challenging to diagnose, even
though histological examination. MicroRNA (miRNA or miRNA) is bound to the target messenger
RNA (mRNA) due to which the mRNA molecules are silenced. The identification of miRNA expression-
based EOC subtypes is considered a critical means of prognostication. So far, the studies on
EOC subtypes have not been well characterized.

Objective:
This study aimed to confirm the existence of miRNAs in EOC and to assess their potential
as clinical biomarkers for EOC.

Methods:
We sampled 25 ovarian tumor tissues from patients with human ovarian tumors (17 malignant;
12 serous EOC, five non-serous EOC, and eight benign ovarian tumors). miRNA microarray
detection was performed to identify EOC miRNAs. Real-time PCR was adapted for the validation of
differentially expressed miRNAs detected by microarray analysis related to hybridization intensity.

Results:
The results confirmed that miRNAs exist in EOC, relative expression of EOC miRNAs
demonstrated that the upregulation of miR-483-5p, and downregulation of miR-127-3p, and miR-
532-5p were significantly expressed in the malignant group than in the benign group at p < 0.05. Besides,
miR-483-5p could also distinguish EOC subtypes between serous EOC and non-serous EOC,
with a p < 0.05.

Conclusion:
A comprehensive miRNA expression profiling can help to refine subtype classification
in EOC, opening new opportunities for identifying clinically applicable markers for improved stratification
and diagnostics of ovarian tumors.