bluuebunny/crossref_metadata_2025_split huggingface dataset:
Factors affecting color stability of maxillofacial prosthetic silicone elastomer: A systematic review and meta-analysis
Authors: Rashid Farah , Barman Aparna , Farook Taseef Hasan , Jamayet Nafij Bin , Yhaya Mohd Firdaus Bin , Alam Mohammad Khursheed 
Published: August 2020

Purpose: This systematic review and meta-analysis explored the factors involved in the color stability and degradation of Maxillo-Facial Prosthetic Silicone Elastomer (MFPSE). Further exploration was done to analyze past literature discussing the potential benefits to color stability when nano-particles were combined with pigmented MFPSE. Method: The search for the articles was done according to PRISMA guidelines. Articles were searched from “Scopus” and “Web of Sciences” from the year 1970 to 2019. Searches were carried out by two reviewers until November 2019. Articles for systematic review were selected based on predefined eligibility criteria. Information regarding weathering conditions, pigments and filler-particle inclusion were extracted as appropriate. Further screening was done for meta-analyze case-control studies of red, blue and yellow pigments according to predefined scoring criteria. Meta-analysis was conducted on case-control studies that incorporated 5%,10% and 15% TiO2 in MFPSE with the said pigments and was carried out using Cochrane Review Manager 5.3. Results: 30 studies were selected for systematic review and 6 studies were eligible for meta-analysis. The most prominent influencers of color stability were nano-fillers and the type of color used in the mixing. Furthermore, experimental conditions, weathering, color of investment plaster and the method of color detection all affected the degree of degradation. There was an overall significant difference found when TiO2 was incorporated with the pigmented silicone. There is significant difference when 10% ( P = 0.0004) TiO2 is incorporated with the red pigment, 5% ( P = 0.03) TiO2 with the yellow and 10% ( P < 0.0001) and 15% ( P = 0.02) TiO2 with the blue pigment. Conclusion: Type of pigment and nano-filler incorporated into the silicone play a role in influencing color stability. Incorporation of 10% TiO2 with red pigment,5% with yellow pigment and 10% or 15% TiO2 with blue pigment provided some protection to the silicone elastomer from color degradation.
Management Options for Patients With Nasopharyngeal Carcinoma Undergoing Cochlear Implantation
Authors: Lee Chee-Yee , Lin Wei-Chieh , Lin Chung-Ching , Hsu Chuan-Jen , Wu Hung-Pin 
Published: August 2020

Objectives: Cochlear implantation (CI) has been successfully used to restore hearing in patients with severe to profound hearing loss. In addition to hearing loss, irradiated patients with nasopharyngeal carcinoma (NPC) also often have chronic otitis media and Eustachian tube (ET) dysfunction. These features may result in difficulties in CI. We conducted this study to review the management options for irradiated patients with NPC undergoing CI. Furthermore, we investigated the results of CI in irradiated patients with NPC with chronic otitis media and obstructive ET dysfunction, accordingly to different treatment strategies. Methods: We retrospectively reviewed the data of patients with NPC who underwent CI in our hospital between 2006 and 2020. All subjects were found to be eligible according to the following inclusion criteria: had NPC, received irradiation, underwent cochlear implant surgery, and had chronic otitis media with obstructive ET dysfunction. Results: A total of 9 patients with obstructive ET dysfunction and chronic otitis media underwent cochlear implant surgery. Seven patients with an inflamed ETs underwent a 2-stage operation. Two patients with obliterated ETs, we performed a single-stage CI surgery. Only 1 patient had otitis media effusion after the surgery. Conclusion: With the proper management of chronic otitis media and ET dysfunction, cochlear implant is an effective treatment for irradiated patients with NPC when conventional hearing aids failed.
Frequency of fruit juice consumption and association with nutrient intakes among Canadians
Authors: Murphy Mary M , Barraj Leila M , Brisbois Tristin D , Duncan Alison M 
Published: August 2020

Background: In Canada, studies on consumption of 100% fruit juice and nutrient intakes are limited. Aim: This study aimed to evaluate nutrient intakes and adequacy of intake by frequency of fruit juice consumption. Methods: Individuals ≥1 year ( n = 34,351) participating in the Canadian Community Health Survey, 2004 with a 24-hour dietary recall and reported usual frequency of fruit juice consumption (assumed to be 100% juice) were categorized by frequency of consumption as <0.5, ≥0.5 to <1.5, or ≥1.5 times/day. Results: More frequent consumption of fruit juice (≥0.5 times/day) was associated with higher intakes of total fruits and vegetables, whole fruits, energy, total sugars, vitamin C and potassium. More frequent consumption of fruit juice was associated with improved intake adequacy of vitamin C for adults. Conclusions: Fruit juice consumption contributes to increased intakes of vitamin C and potassium as well as energy and total sugars, thus presenting a trade-off for consumers to balance.
Evaluation of the In Vitro Uridine Uptake Inhibition Assay in Comparison with the In Vivo Eye Irritation Test as Prescribed by the EEC
Authors: Jacobs Guido A. , Dierickx Paul J. , Martens Mark A. 
Published: August 2020

In vivo eye irritation tests were carried out on 17 substances (mainly solvents) according to the experimental protocols laid down in the European Community legislation on dangerous substances and the OECD Guidelines. Erythema, chemosis, corneal opacity and iritis were observed and evaluated according to the interpretation rules laid down in EEC legislation. The uridine uptake inhibition assay was carried out on Human Hep G2 cells according to the method of Shopsis & Sathe (1), modified by Dierickx & Martens (2). Inhibition of uridine uptake was expressed as the UI50 (Hep G2 cells, mM). Comparison of the results of our two studies revealed only a poor relationship between the UI50 values and the mean scores obtained for corneal opacity (Spearman rank correlation; r=0.57, p<0.02). Consideration of just the monoalcohols, together with the ketones and the formamides, gave a good linear correlation between the UI50 (Hep G2 cells) and the <<Se>> (r=0.93). If a UI50 value of 50mM is accepted as a limit for classification of irritancy for the eye, no false positives or false negatives are obtained. The UI50 can only be used within strictly defined chemical classes, as a first screening method for detecting possible irritants.
Kinetic Studies of Cultured Human Keratinocytes: A Comparison between 5-Bromodeoxyuridine and Tritiated Thymidine Incorporation
Authors: Benassi Luisa , Zambruno Giovanna , Massimi Paola , Fantini Fabrizio , Gianetti Alberto 
Published: August 2020

5-Bromodeoxyuridine (5-BrdU), a pyrimidine analogue of thymidine, is incorporated into the DNA which is actively synthesised during the S-phase of the cell cycle. In the present study, the labelling indices of cultured human keratinocytes, marked by monoclonal antibodies to 5-BrdU and by tritiated thymidine (3H-TdR), were compared. The proportion of cells found to be labelled by the two methods was similar. The immunological method has advantages over 3H-TdR autoradiography, because it does not require radioactive material, and is both faster and less expensive.
An In Vitro Toxicity Assay for Human Endothelial Cells
Authors: Sbarbati Rosella , Schinetti Maria Luisa , Scarlattini Maria 
Published: August 2020

Cultured human endothelial cells can replace living animals in studying the toxic role of noxious agents in the pathogenesis of vascular diseases and in the elucidation of the mechanism of action of protective drugs. Preliminary data are presented which examine the effects that oxidative stress produces on human endothelial cells in vitro. Human umbilical vein endothelial cells were subjected to an anoxia-re-oxygenation treatment and tested for the production of Super Oxide Dismutase (SOD)-inhibitable superoxide radicals. The results show that under our experimental conditions endothelial cells produce oxygen-free radicals and that the generation reaches a maximum after an anoxic challenge of 20 minutes. We conclude that the in vitro system presented in this paper could be a suitable tool for further studies on the effects of oxidative stress on the vascular endothelium, which mimics the in vivo conditions of re-perfusion after heart ischemia.
An Approach to Pain in Research Animals
Authors: Adams David B. 
Published: August 2020

Pain can either signal the threat of tissue-damage (nociception) or can result from tissue damage itself. The physiology and “pathology” of pain, in the second instance, suggest that it can be diagnosed (not “measured”, “assessed”, etc.) on the basis of its association, but not equality, with tissue damage and by its coincidence with changes in behaviour. Pain will be present as part of a syndrome and cannot occur without cause or association. Pain may occur in experiments: a) coincidentally and unrelated to any experimental procedure, b) accidentally, when a procedure goes amiss, and c) as part of the experimental design. Where pain is intrinsic to the aims of an experiment (for example in the testing of analgesics), “escape routes” and “limits” must be formulated for the benefit of experimental subjects. In addition, the empirical value of the experiment must be assessed. There is insufficient information on the use and efficacy of analgesic drugs in animals. These drugs, however, are neither the only nor the most effective means of alleviating physical pain in animals. Other forms of treatment are considered. All depend on sound diagnosis and prognosis. Decisive action against the cause of pain is necessary.
Monoclonal Antibodies: In Vivo and In Vitro Production on a Laboratory Scale, with Consideration of the Legal Aspects of Animal Protection
Authors: Kuhlmann Ingrid , Kurth Waltraud , Ruhdel Irmela 
Published: August 2020

Antibodies in the serum of immunised animals are polyclonal. That is, they react with all determinants of an antigen. Monoclonal antibodies, however, are produced by cells which are all derived from a single antibody-producing cell; hence they are highly specific and react with only one antigenic determinant. Monoclonal antibodies are valuable tools in medical and biological research and can be used for identifying, characterising and purifying medically and biologically important substances. Due to their high specificity, monoclonal antibodies are increasingly used in the diagnosis of infectious disease and neoplasia. Large amounts of antibodies are needed for use in these areas and this necessitates mass production (in the g–kg range); so many authors have described production systems and possibilities for optimising mass production. In contrast, at universities and other research institutions, the production of monoclonal antibodies on a laboratory scale (in the mg range) is still carried out, mostly in the ascites mouse. Certain research areas, require only {minimum amounts μg range) of monoclonal antibodies, so maximising production of antibodies is unnecessary, and as a rule, the yield of a stationary cell culture will suffice. This article summarises the in vivo and in vitro methods described up to now, as well as our own experiences and results. Taking the German animal protection law (22.8.86) as a basis, the legal aspects of animal protection in the production of monoclonal antibodies are discussed. In addition, the legal regulations in Switzerland and the Netherlands are considered.
In Vitro Cytotoxicity of 26 Coded Chemicals to HEp-2 Cells: A Validation Study
Authors: Mazziotti Isabella , Stammati Anna-Laura , Zucco Flavia 
Published: August 2020

Twenty-six chemicals supplied coded by FRAME, were tested on HEp-2 cells. Three endpoints were used to measure the cytotoxicity of these compounds: total protein content, LDH release and total acid phosphatase content. The sensitivity of these parameters is discussed. The results are compared with those obtained by other groups participating in the validation scheme organised by FRAME.
Animal Pain Scales in Public Policy
Authors: Orlans F. Barbara 
Published: August 2020

Pain scales classify the severity of pain inflicted on laboratory animals from little or none up to severe. A pain scale as part of public policy serves beneficial purposes that promote animal welfare. It can be used to educate people about the two alternatives of refinement and replacement, and the need to reduce animal pain. Furthermore, a pain scale has practical applications: 1) in review procedures for animal welfare concerns; 2) in developing policies on the use of animals in education; and 3) as a basis for collecting national data on animal experimentation, so that meaningful data can be collected on trends in reduction and control in animal pain. So far, only a few countries (including Sweden, the Netherlands, Canada and New Zealand) have adopted pain scales as part of their public policy. Most countries, including the United States, have not yet done so. The history of the development and adoption of pain scales by various countries is described and the case is presented for wider adoption of a pain scale in countries not currently using one.
ELISA for Guinea-pig IgG1: An Alternative to the PCA Test for Relative Allergenicity
Authors: Prentø Annette 
Published: August 2020

An ELISA method to replace the PCA (passive cutaneous anaphylaxis) test has been developed for use in the evaluation of relative allergenicity in guinea-pigs (Dunkin-Hartley). The method detects antigen-specific IgG1 antibodies formed in the guinea-pig during sensitisation, and correlates well with the corresponding PCA test. The relative importance of IgG1 and IgE for relative allergenicity in the Dunkin-Hartley guinea-pig strain is discussed.
Two Procedures for the Prediction of Acute Toxicity (LD50) from Cytotoxicity Data
Authors: Halle Willi , Spielmann Horst 
Published: August 2020

Single linear regression analysis was used to characterise the relationship between cytotoxicity in a variety of mammalian cell culture systems and acute oral toxicity (LD50) in experimental animals. The following results were obtained. Firstly, in a cytotoxicity assay using the calf aortic endothelial cell line BKEz-7, IC50 values determined for 44 chemicals in culture showed significant correlation with the oral LD50 values for rat and mouse (computed correlation coefficient r=0.546). After eliminating three chemicals that were characterised by extreme lethality indices (LI = IC50/LD50), the correlation coefficient of the remaining 41 chemicals increased to a value of r=0.728. By using the linear regression model for these 41 chemicals, the oral LD50 for rat and mouse can be predicted correctly from the IC50 values for 83% of substances from a variety of chemical substance classes within a range of approximately one order of magnitude of dosage unit of LD50 for rat and mouse. Secondly, the mean IC50 values (IC50x¯) determined as the geometrical mean of two or more IC50 values per substance, which were generated in a wide spectrum of mammalian cell lines and collected in a “Registry of Cytotoxicity” (RC), gave similar results (r=0.644). Likewise, with the aid of this method, the oral LD50 for rat and mouse can be predicted for 74% of non-selected chemicals from structurally-different classes in the same dosage range, e.g., 1–25 millimoles per kg body weight. The prediction of LD50 values from in vitro cytotoxicity data may permit the calculation of a more precise dose range-finding and offers a new way for reducing the number of animals in acute toxicity testing.
Modification of the FRAME Kenacid Blue Method for Cytotoxicity Tests on Volatile Materials
Authors: Smith Lesley M. , Clothier Richard H. , Hillidge Sonia , Balls Michael 
Published: August 2020

In attempts to overcome the problem for cell culture cytotoxicity tests represented by highly volatile test materials, the wells of 96-well tissue culture plates were sealed individually, either by means of adhesive plastic film spread across the whole plate or by the addition of a small amount of paraffin oil to each well. Use of the adhesive film reduced the growth of control 3T3-L1 murine fibroblast-like cells by nearly 40%, whereas the addition of paraffin oil did not affect cell growth. The latter method was therefore used to compare the cytotoxicities of 27 liquid and 11 water-soluble solid test materials in sealed and unsealed wells, using the FRAME 72-hour kenacid blue method. Of the 27 liquids tested, acetaldehyde, acetone, acetonitrile, allyl alcohol, ethanol, ethyl acetate, methanol, pyrrole and propanol had significantly greater cytotoxic effects in oil-sealed than in unsealed wells. The cytotoxicities of acetic acid, aniline, butanol, 1,2-dibromomethane, dimethyl formamide, dimethyl sulphoxide, glycerol, hydrochloric acid, 2-methoxyethanol, octanol, pentanol, sulphuric acid, tributyltin chloride, trichlorobenzene, trifluoroacetic acid and Tween 20 were not affected by the layer of paraffin oil. Heptanol and hexanol showed significantly lower cytotoxic effects in oil-sealed than in unsealed wells, possibly because of interactions between these alcohols and the oil, since certain alcohols are more soluble in oil than in culture medium. Only one of the 11 solid materials tested, sodium hydroxide, showed greater cytotoxic effects in oil-sealed wells, possibly because of an effect on the culture medium buffering system. The use of paraffin oil as a partial well sealant in 96-well tissue culture plates promises to improve the applicability of in vitro cytotoxicity tests to volatile test materials, by improving confidence that their toxicities have not been underestimated.
The Use of Artemia Salina in Toxicity Testing
Authors: Lewan Lillemor , Andersson Marianne , Morales-Gomez Paloma 
Published: August 2020

This study shows that the Artemia assay, which is usually performed by incubation for a 24-hour period in artificial sea water, can also be performed in phosphate buffered saline (PBS) at pH 7.2, or in a cell culture medium, both of which are used in toxicity assays employing mammalian cells. Thus, by using the equivalent media for incubation, toxic effects in the Artemia assay and toxic mechanisms can more accurately be compared with results obtained in mammalian cell toxicity. Survival of control animals is good for 72 hours, provided that infection can be avoided. A pH greater than 6 is essential for good survival of Artemia Salina, and a pH greater than 10.5 should be avoided. Because of the risk of infection at low saline concentrations, a decreased incubation time of 16 or 12 hours is recommended. The lethal concentrations (LC10 and LC50) in the 24-hour Artemia assay of the first ten chemicals in the MEIC programme were measured in PBS, and the results compared with those from a previous study of the effects in a 10-minute acute ATP leakage assay, specifically indicating lesions in the cell membrane. The EC10 and EC50 values for the alcohols in the Artemia assay were 35–75% of the corresponding values in the ATP leakage assay. For paracetamol and amitriptyline, the EC10 and EC50 values in the Artemia assay were 2–16% of the corresponding values in the ATP-leakage assay. The greatly increased toxicity of the two compounds in the animals may be related to systemic effects. FeSO4 was very toxic to Artemia salina at a concentration of lug/ml, but it did not cause leakage of ATP from cultured cells, even at a concentration of 8,000μg/ml, showing that widely different mechanisms of interaction with FeSO4 are measured by the two assays. A lower toxicity of polygodial, a sesquiterpenoid unsaturated dialdehyde, in cell culture medium, was obvious in the Artemia assay. Thus, some factors in cell culture medium must, by interacting with the toxic molecule, protect the animals against the toxicity of polygodial, as was previously found in cultured cells.
Contributions of Physicochemical Properties to the Evaluation of Ocular Irritation
Authors: Régnier Jean-François , Imbert Christophe 
Published: August 2020

International guidelines for the evaluation of ocular irritation advise that all the available information, structural and physicochemical, should be taken into account in order to minimise the testing of substances under conditions that are likely to produce severe reactions in laboratory animals. In order to determine whether the pH and the acidic/alkaline reserve would be suitable parameters for the prediction of ocular irritation, we have measured, under standard conditions, the pH and the acidic/alkaline reserve of 166 chemicals (90 non-irritant, 22 irritant, and 54 severely irritant for the eye, according to the EEC classification and labelling system). The combination of these parameters permitted the correct identification of 74% of the severely irritant chemicals and 97% of the non-severely irritant (non-irritant or irritant) chemicals. The predictive value for a severely irritant potential was 93%, and 88% for non-severely irritant chemicals. This physicochemical approach, used as a first line screen for ocular irritation, is very simple and inexpensive.
Evaluation of the EYTEX™ System as a Screening Method for Ocular Tolerance: Application to Raw Materials and Finished Products
Authors: Courtellemont Pascal , Hebert Patrice , Redziniak Gerard 
Published: August 2020

In vitro evaluation of ocular irritation requires the use of a battery of tests, for example cell culture assays and the HET-CAM test. In order to check the field applications of the different methods and their complementation, we have studied the EYTEX™ physicochemical model in an internal validation study. In this study, we compared the EYTEX™ results with the in vitro ocular irritation potencies of 52 formulations and 49 chemicals. The in vivo/in vitro correlation was highly significant, p < 0.01%, K = 0.8 (Kappa method). On the other hand, the concordance, sensitivity and specificity of this method were, 85%, 89% and 83% respectively. The compatability of 98% obtained showed the kit to be applicable to cosmetic formulations. The EYTEX™ system has also been evaluated during a comparative study of EYTEX™ human clinical tests, performed on 12 finished products; we have observed a concordance equal to 92%, indicating an excellent correlation between the two methodologies. These results suggest that the EYTEX™ test could be a reliable in vitro prescreening system for determining the eye irritation potential of cosmetic formulations, and that it should be accepted as a component of our battery of alternative methods.