bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of a complex mixture prepared from agrimonia, houttuynia, licorice, peony, and phellodendron on human skin cells
Authors: Lee Kyung-Ha , Lee Jeong Pyo , Kim Wanil 
Published: December 2020

AbstractActive ingredients derived from natural sources are widely utilized in many industries. Cosmetic active ingredients are largely derived from various plants. In this study, we examined whether a mixture of plant extracts obtained from agrimonia, houttuynia, licorice, peony, and phellodendron (hereafter AHLPP), which are well-known for their effects on skin, could affect skin barrier function, inflammation, and aging in human skin cells. We also determined whether AHLPP extracts sterilized using γ-irradiation (to avoid preservatives) retained their skin cell regulating activity. The AHLPP mixture could downregulate representative pro-inflammatory cytokines including IL 1-β and IL 7. Procollagen peptide synthesis was also increased by AHLPP treatment along with mRNA upregulation of barrier proteins such as filaggrin and desmoplakin. The AHLPP mixture showed an anti-aging effect by significantly upregulating telomerase activity in human keratinocytes. We further observed TERT upregulation and CDKN1B downregulation, implying a weakening of pro-aging signal transduction. Co-cultivation of a hydrogel polymer containing the AHLPP mixture with human skin cells showed an alteration in skin-significant genes such as FLG, which encodes filaggrin. Thus, the AHLPP mixture with or without γ-irradiation can be utilized for skin protection as it alters the expression of some significant genes in human skin cells.
Phylogenetic comparisons reveal mosaic histories of larval and adult shell matrix protein deployment in pteriomorph bivalves
Authors: Zhao Ran , Takeuchi Takeshi , Koyanagi Ryo , Villar-Briones Alejandro , Yamada Lixy , Sawada Hitoshi , Ishikawa Akito , Iwanaga Shunsuke , Nagai Kiyohito , Che Yuqi , Satoh Noriyuki , Endo Kazuyoshi 
Published: December 2020

AbstractMolluscan shells are organo-mineral composites, in which the dominant calcium carbonate is intimately associated with an organic matrix comprised mainly of proteins and polysaccharides. However, whether the various shell matrix proteins (SMPs) date to the origin of hard skeletons in the Cambrian, or whether they represent later deployment through adaptive evolution, is still debated. In order to address this issue and to better understand the origins and evolution of biomineralization, phylogenetic analyses have been performed on the three SMP families, Von Willebrand factor type A (VWA) and chitin-binding domain-containing protein (VWA-CB dcp), chitobiase, and carbonic anhydrase (CA), which exist in both larval and adult shell proteomes in the bivalves,Crassostrea gigasandPinctada fucata. In VWA-CB dcp and chitobiase, paralogs for larval and adult SMPs evolved before the divergence of these species. CA-SMPs have been taken as evidence for ancient origins of SMPs by their presumed indispensable function in biomineralization and ubiquitous distribution in molluscs. However, our results indicate gene duplications that gave rise to separate deployments as larval and adult CA-SMPs occurred independently in each lineage after their divergence, which is considerably more recent than hitherto assumed, supporting the “recent heritage and fast evolution” scenario for SMP evolution.
Identification and validation of a miRNA-based prognostic signature for cervical cancer through an integrated bioinformatics approach
Authors: Qi Yumei , Lai Yo-Liang , Shen Pei-Chun , Chen Fang-Hsin , Lin Li-Jie , Wu Heng-Hsiung , Peng Pei-Hua , Hsu Kai-Wen , Cheng Wei-Chung 
Published: December 2020

AbstractCervical cancer is the fourth most common cancer in women worldwide. Increasing evidence has shown that miRNAs are related to the progression of cervical cancer. However, the mechanisms that affect the prognosis of cancer are still largely unknown. In the present study, we sought to identify miRNAs associated with poor prognosis of patient with cervical cancer, as well as the possible mechanisms regulated by them. The miRNA expression profiles and relevant clinical information of patients with cervical cancer were obtained from The Cancer Genome Atlas (TCGA). The selection of prognostic miRNAs was carried out through an integrated bioinformatics approach. The most effective miRNAs with synergistic and additive effects were selected for validation through in vitro experiments. Three miRNAs (miR-216b-5p, miR-585-5p, and miR-7641) were identified as exhibiting good performance in predicting poor prognosis through additive effects analysis. The functional enrichment analysis suggested that not only pathways traditionally involved in cancer but also immune system pathways might be important in regulating the outcome of the disease. Our findings demonstrated that a synergistic combination of three miRNAs may be associated, through their regulation of specific pathways, with very poor survival rates for patients with cervical cancer.
T-cell receptor repertoire of cytomegalovirus-specific cytotoxic T-cells after allogeneic stem cell transplantation
Authors: Toya Takashi , Taguchi Ayumi , Kitaura Kazutaka , Misumi Fumi , Nakajima Yujiro , Otsuka Yuki , Konuma Ryosuke , Adachi Hiroto , Wada Atsushi , Kishida Yuya , Konishi Tatsuya , Nagata Akihito , Yamada Yuta , Marumo Atsushi , Noguchi Yuma , Yoshifuji Kota , Mukae Junichi , Inamoto Kyoko , Igarashi Aiko , Najima Yuho , Kobayashi Takeshi , Kakihana Kazuhiko , Ohashi Kazuteru , Suzuki Ryuji , Nagamatsu Takeshi , Doki Noriko 
Published: December 2020

AbstractCytomegalovirus (CMV) infection is a major complication during allogeneic stem cell transplantation (allo-SCT). However, mechanisms of adaptive immunity that drive this remain unclear. To define early immunological responses to CMV after transplantation, we using next-generation sequencing to examine the repertoire of T-cell receptors in CD8+/CMV pp65 tetramer+ cells (CMV-CTLs) in peripheral blood samples obtained from 16 allo-SCT recipients with HLA-A*24:02 at the time of CMV reactivation. In most patients, TCR beta repertoire of CMV-CTLs was highly skewed (median Inverse Simpson’s index: 1.595) and, 15 of 16 patients shared at least one TCR-beta clonotype with ≥ 2 patients. The shared TCRs were dominant in 12 patients and, two clonotypes were shared by about half of the patients. Similarity analysis showed that CDR3 sequences of shared TCRs were more similar than unshared TCRs. TCR beta repertoires of CMV-CTLs in 12 patients were also analyzed after 2–4 weeks to characterize the short-term dynamics of TCR repertoires. In ten patients, we observed persistence of prevailing clones. In the other two patients, TCR repertoires became more diverse, major clones declined, and new private clones subsequently emerged. These results provided the substantive clue to understand the immunological behavior against CMV reactivation after allo-SCT.
Upregulation of 5′-terminal oligopyrimidine mRNA translation upon loss of the ARF tumor suppressor
Authors: Cottrell Kyle A. , Chiou Ryan C. , Weber Jason D. 
Published: December 2020

AbstractTumor cells require nominal increases in protein synthesis in order to maintain high proliferation rates. As such, tumor cells must acquire enhanced ribosome production. How the numerous mutations in tumor cells ultimately achieve this aberrant production is largely unknown. The gene encoding ARF is the most commonly deleted gene in human cancer. ARF plays a significant role in regulating ribosomal RNA synthesis and processing, ribosome export into the cytoplasm, and global protein synthesis. Utilizing ribosome profiling, we show that ARF is a major suppressor of 5′-terminal oligopyrimidine mRNA translation. Genes with increased translational efficiency following loss of ARF include many ribosomal proteins and translation factors. Knockout of p53 largely phenocopies ARF loss, with increased protein synthesis and expression of 5′-TOP encoded proteins. The 5′-TOP regulators eIF4G1 and LARP1 are upregulated in Arf- and p53-null cells.
Deposited ultra-thin titanium nitride nanorod array as a plasmonic near-perfect light absorber
Authors: Jen Yi-Jun , Yang Kai-Bin , Lin Po-Chun , Chung Meng-Hsun 
Published: December 2020

AbstractThe transmittance, reflectance, and extinctance that correspond to the localized plasmonic resonance within TiN nanorods were investigated. The obliquely deposited TiN nanorod array shows polarization-independent admittance matching to air. Unlike noble metal nanorods, the near-field localized longitudinal and transverse plasmonic resonance of TiN nanorod arrays present polarization-dependent light extinction in the far field. The longitudinal plasmonic mode presents stronger extinction than transverse plasmonic mode. In order to have high efficient light absorption, an ultra-thin two-layered TiN nanorod array was fabricated with orthogonal deposition planes for upper layer and bottom layer to absorb different polarized light energy. The measured spectrum shows broadband and wide-angle light extinction.
The Efficacy of an Explicit Intervention Approach to Improve Past Tense Marking for Early School-Age Children With Developmental Language Disorder
Authors: Calder Samuel D. , Claessen Mary , Ebbels Susan , Leitão Suze 
Published: December 2020

Purpose
The aim of the study was to evaluate the efficacy of a theoretically motivated explicit intervention approach to improve regular past tense marking for early school-age children with developmental language disorder (DLD).

Method

Twenty-one children with DLD (ages 5;9–6;9 [years;months]) were included in a crossover randomized controlled trial (intervention,
n
= 10; waiting control,
n
= 11). Intervention included once-weekly sessions over 10 weeks using the SHAPE CODING system, in combination with a systematic cueing hierarchy to teach past tense marking. Once the first group completed intervention, the waiting control group crossed over to the intervention condition. The primary outcome was criterion-referenced measures of past tense marking with standardized measures of expressive and receptive grammar as the secondary outcome. Ancillary analyses on extension and behavioral control measures of morphosyntax were also conducted.

Results

There was a significant Time × Group interaction (
p
< .001) with a significant difference in pre–post intervention improvement in favor of the intervention group (
p
< .001,
d
= 3.03). Further analysis once both groups had received the intervention revealed no improvement for either group on past tense production during the 5-week pre-intervention period, significant improvement pre–post intervention (
p
<
.
001,
d
= 1.22), with gains maintained for 5 weeks postintervention. No significant differences were found on pre- to postintervention standardized measures of grammar, or on extension or control measures.

Conclusions
The efficacy of the theoretically motivated explicit grammar intervention was demonstrated. Results contribute to the evidence base supporting this intervention to improve past tense production in early school-age children with DLD, suggesting it is a viable option for clinicians to select when treating morphosyntactic difficulties for this population.

Supplemental Material

https://doi.org/10.23641/asha.13345202
High stability perovskite solar cells under ambient conditions
Authors: Aslan Emre , Ates Turkmen Tulin , Alturk Elif 
Published: December 2020

Though perovskite solar cells have outstanding commercialisation potential, perovskite materials are very vulnerable to moisture and oxygen. So the main issue for perovskite solar cell is very fast degradation. In order to elaborate on this concern, the stability of perovskite solar cells were investigated in this study. The effect of oxygen and moisture on perovskite solar cells decreased with encapsulation of the cells. Encapsulation has been carried out with ultraviolet cured epoxy resin. Stability tests were taken place in laboratory conditions under solar simulator and also shelf‐life tests. Perovskite solar cells showed the highest power conversion efficiency (PCE) of 9.77% with a short circuit current density of 22.5 mA/cm2, the open‐circuit voltage of 0.94 V and fill factor of 0.46. After 2800 h, perovskite solar cells were still 85% stable in atmospheric conditions. Perovskite solar cells exhibited distinctive durability for shelf‐life test in laboratory conditions. The PCE of perovskite solar cells were decreased only 15% of its initial value after 2800 h. In order to elaborate the degradation of perovskite solar cells, the cells are exposed to solar irradiation AM1.5G. Perovskite solar cells degraded gradually with time under solar irradiation; however, this stability test indicated that the solar cells were durable 20% after 500 h exposure to solar irradiation.
Impact of a Heutagogical, Multimedia-Based Teaching Concept to Promote Self-Determined, Cooperative Student Learning in Clinical Radiology
Authors: Teichgräber Ulf , Ingwersen Maja , Mentzel Hans-Joachim , Aschenbach Rene , Neumann Rotraud , Franiel Tobias , Herzog Aimée Barbara , Böttcher Joachim , Pfeil Alexander , Mensel Birger , Kühnel Christian , Freesmeyer Martin , Fischer Martin R. , Zottmann Jan 
Published: December 2020

Purpose To date, didactic lecturing is a common method of university medical training. However, higher levels of competence to solve complex issues are hardly to be achieved with a largely passive learning style. We established and evaluated a heutagogical blended learning concept to investigate self-determined learning with a multimedia-based, interactive approach in the lecture room to teach clinical radiology.
Materials and Methods In the 2019/2020 winter semester, we included 266 medical students in their fourth academic year in our prospective, observational study. Students participated in a series of 11 radiological lectures given by 10 lecturers. They were requested to prepare for lectures by watching learning videos. During the lecture, students had to answer key-feature questions (KFQ) in small groups and to jointly submit their answers by means of an audience response system (ARS). After each lecture and the exam, we conducted surveys and compared results with a historical control group. A focus group interview with lecturers was performed after conclusion of the lecture series.
Results The students’ overall impression of the “flipped classroom” concept and their examination grades were superior to historical controls (overall impression: 1.5 [95 % CI 1.4–1.6] vs. 2.7 [95 % CI 2.5–2.9] rated on a scale from 1 to 6, p < 0.001; examination grades: 1.8 [95 % CI 1.7–1.9] vs. 2.0 [95 % CI 1.9–2.0] rated on a scale from 1 to 5, p < 0.001). Most students agreed that learning videos (76.6 %), ARS (88.5 %), KFQ (76.5 %), and solution-oriented small group discussions (83.7 %) were useful. Lecturers stated an improved convergence of demands on learning and clinical competence. However, they also emphasized an increased initial effort for implementation.
Conclusion Students rated the overall benefit from the heutagogical “flipped classroom” concept as high. Examination grades improved. According to lecturers, the “flipped classroom” concept better matched later professional demands than traditional lectures.
Key Points:
Citation Format
Neue Klassifikation des Diabetes mellitus – Anforderungen an Labormessgrößen
Authors: Hörber Sebastian , Kaiser Patricia , Achenbach Peter , Schleicher Erwin , Peter Andreas 
Published: December 2020

ZusammenfassungDie bislang etablierte Klassifikation des Diabetes mellitus hat sich in der Praxis zwar bewährt, spiegelt aber die Heterogenität der Erkrankung nicht wider. In einer Clusteranalyse einer schwedischen Arbeitsgruppe wurde eine neue Klassifikation vorgeschlagen, die den Diabetes mellitus in 5 Subgruppen unterteilt. Diese unterscheiden sich maßgeblich in der Prädiktion und Therapie von Folgeerkrankungen von Menschen mit Diabetes. Zur Klassifikation wurden unter anderem GAD-Autoantikörper, HbA1c, HOMA2-B und HOMA2-IR verwendet, wobei die Berechnung der HOMA-Indizes auf den Messgrößen Glukose und Insulin beziehungsweise C-Peptid beruht. Die Messverfahren der dabei verwendeten Labormessgrößen müssen notwendigerweise standardisiert und damit vergleichbar sein. Erst dann kann diese Klassifikation weltweit angewendet werden. Unabhängig davon ist die Vergleichbarkeit von Laborergebnissen auch für die Diagnosestellung, Prädiktion und Therapiesteuerung von Menschen mit Diabetes zwingend notwendig. In den letzten Jahrzehnten wurden daher bei den diabetesrelevanten Labormessgrößen große Anstrengungen unternommen, um eine Standardisierung dieser Parameter zu erreichen. Für Glukose und HbA1c-Wert konnte dies inzwischen erreicht werden, sodass diese Parameter international vergleichbar gemessen werden. Der Prozess der Standardisierung der C-Peptid-Bestimmung soll 2020 fertiggestellt sein und anschließend umgesetzt werden. Dagegen ist die Standardisierung der Insulinbestimmung bisher nur unzureichend fortgeschritten. Die Bestimmung von GAD-Autoantikörpern kann aufgrund der biologischen Heterogenität von Autoantikörpern nicht standardisiert werden, jedoch ist in den letzten Jahren eine weitgehende Harmonisierung erzielt worden. Durch eine weltweite Standardisierung dieser Parameter können fehlerhafte Diagnosen und falsche klinische Entscheidungen, die auf Labormessgrößen beruhen, zum Nutzen der Patienten reduziert werden.
Enseñanza en Investigación en Urología. Análisis Bibliométrico
Authors: Roa Veronica Tobar , López Beatriz Johanna Vega , Solano Catalina , Carvajal Luisana Castillo , Pulido Joan Stefany Duque , Yepes Catalina Gonzalez , Barbosa Pamela Perdomo 
Published: December 2020

Resumen
Objetivo Caracterizar el panorama de la educación en investigación en urología mediante un análisis bibliométrico.
Métodos Realizamos un análisis bibliométrico, utilizando artículos publicados entre 1955- 2019, sin restricción en el idioma. Se utilizaron las herramientass estadísticass PubMed, FABUMED y PubReminer para la obtención de la información de las variables y realización del análisis bibliométrico. Analisis de mapeo utilizando el software: VOSviewer,. Para la obtención del factor de impacto (FI), se utilizó el Journal Citation Reports 2017/2018.
Resultados Desde 1955 hasta 2019 se publicaron 718 referencias en 245 revistas. A partir del 2000 encontramos un aumento significativo en el número de publicaciones con un punto de corte en el año 2009. Las revistas con mayores publicaciones fueron: J Urol (8.6%), BJU Int (6.8%) y Urology (6.5%). De las 20 revistas con mayor número de publicaciones, sólo desolo 5 un FI mayor a 3. Los paises con mayor publicacion fueron: Estados Unidos (23,6%), Reino Unido (20,2%). El país de América Latina con mayor publicacion fué Brasil (0,8%).
Conclusiones El estado de la educación en investigación en urología demuestra un crecimiento en la producción científica , con una baja contribución por parte de los países latinoamericanos. Para poder desarrollar una creación intelectual de calidad se deberá invertir tiempo y recursos en un adecuado entrenamiento en investigación en los programas de formación en urología.
How Docket Control Shapes Judicial Behavior: A Comparative Analysis of the Norwegian and Danish Supreme Courts
Authors: Skiple Jon Kåre , Bentsen Henrik Litleré , McKenzie Mark Jonathan 
Published: December 2020

AbstractEuropean courts have responded to increasing caseloads by providing justices or other actors with a higher degree of discretionary docket control. Does docket type—mandatory or discretionary—shape judicial behavior? Using a most similar systems research design regarding tax decisions in the Norwegian and Danish supreme courts, we show that discretionary dockets are associated with higher dissent and reversal rates than mandatory dockets, that low-status litigants have a lower chance of winning under mandatory dockets, and that docket type conditions the effects of justices’ preferences. Our findings have implications for comparative judicial politics and for institutional design.
Inverse engineering of electromagnetically induced transparency in terahertz metamaterial via deep learning
Authors: Huang Wei , Wei Ziming , Tan Benying , Yin Shan , Zhang Wentao 
Published: December 2020

Abstract
In this paper, we apply the deep learning network to the inverse engineering of electromagnetically induced transparency (EIT) in terahertz metamaterial. We take three specific points of the EIT spectrum with six inputs (each specific point has two physical values with frequency and amplitude) into the deep learning model to predict and inversely design the geometrical parameters of EIT metamaterials. We propose this algorithm for the general inverse design of EIT metamaterials, and we demonstrate that our method is functional by taking one example structure. Our deep learning model exhibits a mean square error of 0.0085 in the training set and 0.014 in the test set. We believe that this finding will open a new approach for designing geometrical parameters of EIT metamaterials, and it has great potential to enlarge the applications of the THz EIT metamaterial.
Electrical conductivity enhancement of transparent silver nanowire films on temperature-sensitive flexible substrates using intense pulsed ion beam
Authors: Kaikanov Marat , Kemelbay Aidar , Amanzhulov Bauyrzhan , Demeuova Gulzat , Akhtanova Gulnur , Bozheyev Farabi , Tikhonov Alexander 
Published: December 2020

Abstract
Silver nanowire (AgNW) networks have attracted particular attention as transparent conductive films (TCF) due to their high conductivity, flexibility, transparency, and large scale processing compatible synthesis. As-prepared AgNW percolating networks typically suffer from high contact resistance, requiring additional post-synthetic processing. In this report, large area irradiation with 200 ns short intense pulsed ion beam (IPIB) was used to anneal and enhance the conductivity of AgNW network, deposited on temperature-sensitive polyethylene terephthalate (PET) substrate. A TCF sheet resistance shows irradiation dose dependence, decreasing by four orders of magnitude and reaching a value of 70 Ω/sq without damaging the polymer substrate, which retained a transparency of 94%. The IPIB irradiation fused AgNW network into the PET substrate, resulting in a great adhesion enhancement. Heat transfer simulations show that the heat originates at the near-surface layer of the TCF and lasts an ultra-short period of time. Obtained experimental and simulation results indicate that the irradiation with IPIBs opens new perspectives in the low-temperature annealing of nanomaterials deposited on temperature-sensitive substrates.
First-principles study of sulfur vacancy concentration effect on the electronic structures and hydrogen evolution reaction of MoS2
Authors: Zhou Wenyu , Dong Lichun , Tan Luxi , Tang Qing 
Published: December 2020

Abstract
Defect engineering has been widely used in experiments to modulate the electrocatalytic properties of molybdenum disulfide (MoS2). However, the effect of vacancy concentration on the vacancy distribution, electronic properties, and hydrogen evolution reaction (HER) activity remains elusive. Herein, we perform density functional theory (DFT) studies to investigate defective MoS2 with different numbers of sulfur vacancies. In the case of low S-vacancy concentration, the vacancies prefer to agglomerate rather than being dispersed, while at the higher-vacancy concentration, the combination of local point defect and clustered vacancy chain is preferred. The coupling between S-vacancies leads to decreased band gap and increased Mo–H adsorption strength with increasing vacancy concentration. The optimal HER activity is identified to occur below vacancy concentration of 12.50%. Our work provides an atomic-level understanding about the role of S-vacancies in the HER performance of MoS2, and offers useful guidelines for the design of defective MoS2 and other TMDs electrocatalysts.