bluuebunny/crossref_metadata_2025_split huggingface dataset:
Treatment of asymptomatic pyuria in psychiatric patients discharged from the emergency department following antimicrobial stewardship implementation
Authors: Ellena Kamah , Wolf Lauren , Dumkow Lisa E. , VanLangen Kali M. 
Published: July 2020

AbstractIntroductionAbnormal urinalysis (UA) results, such as pyuria or bacteriuria, increase the risk for antibiotic treatment even in the absence of urinary symptoms. In the emergency department (ED), patients presenting with psychiatric emergencies commonly undergo a medical assessment which includes a UA. We hypothesized that the rate of inappropriate antibiotic treatment for asymptomatic pyuria in the psychiatric medical population would decrease following implementation of an antimicrobial stewardship program (ASP) in the ED.MethodsThis retrospective cohort study compared the treatment of adult patients presenting to the ED for medical assessment during a psychiatric emergency with noted pyuria between three time periods following implementation of an ASP: early ASP (2014), established ASP (2016), and mature ASP (2018). The ASP provided education and routine audit‐and‐feedback focused on reducing urine testing and eliminating treatment of asymptomatic pyuria. The primary end point was to compare rates of inappropriate treatment of asymptomatic pyuria between groups. Secondary end points included comparing appropriate agent selection and duration for patients who were prescribed antibiotics, prescriber documentation, and patient outcomes between groups.ResultsA total of 180 patients with pyuria were included; 60 within each study group with 88.3% being asymptomatic. Inappropriate prescribing of antibiotics for asymptomatic patients at ED discharge decreased over time following ASP implementation (2014 = 40.4%, 2016 = 31.4%, 2018 = 17.6%; P = .036). Additionally, documentation noting a positive UA requiring antibiotic treatment decreased (2014 = 46.7%, 2016 = 40%, 2018 = 26.7%). Of the 62 patients treated with antibiotics, selection of a first‐line agent increased with ASP maturity (2014 = 15.4%, 2016 = 26.1%, 2018 = 69.9%; P = .002).ConclusionInappropriate treatment of asymptomatic pyuria in patients presenting to the ED with psychiatric emergencies decreased significantly over time following implementation of an ASP. EDs may benefit from ASP interventions aimed at decreasing reliance on UA interpretation in this patient population.
Peri‐tumoural stroma collagen organization of invasive ductal carcinoma assessed by polarized light microscopy differs between OncotypeDX risk group
Authors: Jones Blake , Thomas Georgia , Sprenger Jillian , Nofech‐Mozes Sharon , Khorasani Mohammadali , Vitkin Alex 
Published: July 2020

AbstractA commercially available genomic test, OncotypeDX has emerged as a useful postsurgical treatment guide for early stage breast cancer. Despite widespread clinical adoption, there remain logistical issues with its implementation. Collagenous stromal architecture has been shown to hold prognostic value that may complement OncotypeDX. Polarimetric analysis of breast cancer surgical samples allows for the quantification of collagenous stroma abundance and organization. We examine intratumoural collagen abundance and alignment along the tumor‐host interface for 45 human samples of invasive ductal carcinoma categorized as low or higher risk by OncotypeDX. Furthermore, we probe the separatory power of collagen alignment patterns to classify unlabeled samples as low or higher OncotypeDX risk group using a linear discriminant (LD) model. No significant difference in mean collagen abundance was found between the two risk groups. However, collagen alignment along the tumor boundary was found to be significantly lower in higher risk samples. The LD model achieved a 71% total accuracy and 81% sensitivity to higher risk samples. Prognostic information extracted from the stromal morphology has potential to complement OncotypeDX as an easy‐to‐implement prescreening methodology.
An alternative procedure to leukapheresis for peripheral hematopoietic progenitor cell collection in very‐low‐weight children: A single pediatric center experience
Authors: Becherucci Valentina , Ermini Stefano , Piccini Luisa , Bisin Silvia , Gori Valentina , Gentile Francesca , Ceccantini Riccardo , De Rienzo Elena , Bindi Barbara , Pavan Paola , Cunial Vanessa , Allegro Elisa , Brugnolo Francesca , Maggio Daniela , Calzolari Daniela , Maccarelli Erika , Galli Silvia , Muricci Sonia , Berchielli Marco , Tintori Veronica , Sardi Iacopo , Bambi Franco 
Published: July 2020

AbstractBackgroundPBSC collection using a blood cell separator in very low weight patients can be frequently complicated by severe adverse effects and technical difficulties.Material and MethodsFrom March 2013 to January 2017, 14 PBSC collections were performed in 12 children weighing less than 10 kg, affected by different solid tumours. PBSC collection was performed with a “homemade” aseptically assembled circuit. The circuit is composed by a 150 mL collection bag connected with a 4 stopcock ramp, perfused with ACD. This circuit allows collection of a specific total blood amount from CVC, depending on CD34+/kg target.ResultsMean CD34+cell performance per collection was 9.3 × 106/kg. Tolerance to the procedure was very good as none of the patients experienced complications, with the exception of a patient who showed mild cyanosis and pallor after collection. Moreover, no bleeding or thrombotic complications have been observed. To date, 16 PBSC reinfusions have been performed in 7 children with a mean CD34+cells viability of 98.1% ± 2.7 and mean WBC viability of 57% ± 10. Cell recovery after thawing was 87% ± 10.8. A rapid graft intake for both neutrophils and platelets, between day 7 and 20 after reinfusion was observed.DiscussionThe procedure of total blood collection without the use of a cell separator is feasible and allows a good PBSC collection without significant side effects in very low‐weight children. Moreover, this method could represent a valid and safe alternative to leukapheresis in patients where classic procedure could be difficult to apply.
Severe renal impairment as an adverse prognostic factor for survival in newly diagnosed multiple myeloma patients
Authors: Chen Xuduan , Luo Xiaofeng , Zu Yanping , Issa Hajji Ally , Li Linlin , Ye Hong , Yang Ting , Hu Jianda , Wei Lixin 
Published: July 2020

AbstractBackgroundRenal impairment (RI) is associated with poor survival in newly diagnosed multiple myeloma (MM) patients. Renal function recovery has been one of the main therapeutic goals in those patients.MethodsThe records from 393 newly diagnosed MM patients in our hospital between January 2012 and December 2016 were retrospectively analyzed. RI was defined as an eGFR < 40 mL/min according to the novel IMWG criteria. RI patients were categorized based on their renal function at diagnosis: severe RI: eGFR < 30 mL/min, and mild RI: 30 mL/min ≤ eGFR <40 mL/min. We explored whether RI, and particularly severe RI, was an adverse prognostic factor for survival, and investigated the impact of renal function recovery on survival.ResultsSevere RI, hemoglobin <100 g/L, LDH ≥ 245 U/L, hyperuricemia, 1q21 amplification, and lack of novel agent treatment were associated with decreased overall survival (OS). Severe RI patients with renal response had a median OS of 27 months compared with 18 months for those patients without renal response (P = .030), but their median OS was still significantly lower than that for patients without severe RI, which was 51 months. In severe RI patients, the overall renal response rate in bortezomib‐based regimens was significantly higher than that in nonbortezomib‐based regimens.ConclusionOur results suggest that severe RI is an adverse prognostic factor for survival in newly diagnosed MM patients, restoration of renal function may improve survival, and bortezomib‐based regimens may be the preferred treatment in patients with severe RI.
The association of miR‐27a rs895819 polymorphism with colorectal cancer risk in Chinese population
Authors: Zhang Shulong , Han Qi , Zhu Kaihua , Wang Quan 
Published: July 2020

AbstractBackgroundBesides environment and living habits, such as a sedentary lifestyle, smoking and drinking, genetic variation also plays an important role in the development of colorectal cancer (CRC). This study was aimed to investigate the role of miR‑27a rs895819 polymorphism on CRC risk in Chinese population.MethodsIn a case‐control study including 208 CRC and 312 age‐ and gender‐matched healthy control subjects, the rs895819 polymorphism was genotyped using the TaqMan allelic discrimination assay. Furthermore, a pooled analysis based on eligible studies was performed by using the STATA software.ResultsLogistic regression analysis showed that the rs895819 polymorphism was not associated with CRC risk. However, a pooled analysis based on five studies from Chinese population showed a statistically significant association between the rs895819 polymorphism and CRC risk (GG vs AA: OR = 1.56, 95% CI = 1.27‐1.92, Pz < .01; (AG + GG) vs AA: OR = 1.14, 95% CI = 1.01‐1.30, Pz = .04; GG vs (AG + AA): OR = 1.54, 95% CI = 1.27‐1.88, Pz < .01; G vs A: OR = 1.20, 95% CI = 1.09‐1.33, Pz < .01).ConclusionOur study suggests that miR‑27a rs895819 polymorphism plays an important role in CRC risk in Chinese population and may serve as a valuable biomarker for predicting an individual's susceptibility to CRC.
Bioinformatic identification of key pathways, hub genes, and microbiota for therapeutic intervention in Helicobacter pylori infection
Authors: Chen Zhenhui , Chen Huijuan , Yu Lu , Xin Hongjie , Kong Jingjing , Bai Yang , Zeng Weisen , Zhang Jumei , Wu Qingping , Fan Hongying 
Published: July 2020

AbstractThe pathogenic mechanisms of Helicobacter pylori infection remain to be defined, and potential interventional microbiota are just beginning to be identified. In this study, gene‐set enrichment analysis (GSEA) was used to integrate three H. pylori infection microarray data sets from the gene expression omnibus database and identified ten hallmark gene sets and 35 Kyoto encyclopedia of genes and genomes (KEGG) pathways that differed between healthy and Helicobacter pylori‐infected individuals. Weighted gene co‐expression network analysis (WGCNA) performed on two of the data sets identified three key gene coexpression modules. These modules contained 54 enriched KEGG pathways, 25 of which overlapped with the GSEA analysis, suggesting potentially important roles in H. pylori‐infection. We selected 116 hub genes from the three key modules for in vitro validation at the transcriptional level using H. pylori Sydney Strain 1 and verified the upregulation of 80. WGCNA of the microbiomes based on 20 mucosal samples and a sequence read archive data set revealed four microbiota modules correlated with H. pylori infection. The negatively correlated modules contained 11 microbiome families. These findings provide new insight into the pathogenesis of H. pylori infection and systematically identify 25 key pathways, 80 upregulated hub genes, and 11 families of candidate interventional microbiota for further research.
Serum-borne lipids amplify TLR-activated inflammatory responses
Authors: Sharma Naveen , Akhade Ajay Suresh , Ismaeel Sana , Qadri Ayub 
Published: July 2020

Abstract
TLRs recognize conserved pathogen associated molecular patterns and generate innate immune responses. Several circulating and cell membrane associated proteins have been shown to collaborate with TLRs in sensing microbial ligands and promoting inflammatory responses. Here, we show that serum and serum-borne lipids including lysophosphatidylcholine (LPC) amplify inflammatory responses from intestinal epithelial cells and mononuclear phagocytes primed with microbial TLR ligands. Treatment with the inhibitors of G protein-coupled receptor (GPCR) signaling, suramin, or pertussis toxin (PT), the inhibitor of JNK-MAPK, or knockdown of LPC response-regulating GPCR, G2A, decreases the augmentation brought about by serum or LPC in TLR-induced inflammatory response. In vivo administration of PT or anti-G2A antibody reduces TLR2-activated cytokine secretion. The ability of host lipids to costimulate TLR-generated cellular responses represents a novel pathway for the amplification of innate immunity and inflammation.
Development of a new atmospheric pressure plasmaspray ionization for ambient mass spectrometry
Authors: Peng Zhen , Liu Ji‐Xing , Zhu Hui , Guo Teng , Xu Li , Dong Jun‐Guo , Cheng Ping , Zhou Zhen 
Published: July 2020

AbstractA new atmospheric pressure ionization method, plasmaspray ionization, termed as PSI, was developed to be an alternative ambient ion source for mass spectrometry. It comprises a plasma jet device and a sample spray part. While the nonthermal plasma jet strikes the surface of stainless steel tube out of the spray capillary, the sprayed sample will be ionized with the assistant of auxiliary gas. Although PSI is a little bit more complex than electrospray ionization (ESI) in instrument, it shows both better linearity and higher sensitivity for organic compounds. For protein samples, it presents wider distributions of multiply charged ions and higher mass resolution without sacrificing any sensitivity. For the mechanism of PSI, the charge build‐up process on the tip of capillary should play a key role for the ion formation, and the stimulated pulsed voltage on the flow tube will promote the ion aggregation speed until the charge density is high enough. PSI source contains the features of plasma ionization and ESI and can be considered as a novel combo bridging these techniques. These results reflect that this method of PSI can be applied and further developed as a versatile new ion source for a wild range of organic and biological samples.
Antibody responses to SARS‐CoV‐2 in healthy individuals returning to Shenzhen
Authors: Zhang Cantong , Lin Liancheng , Tang Donge , Liu Fuju , Li Meng , Li Qiongying , Deng Zhaoxiang , Chu Guohai , Gu Dayong , Dai Yong 
Published: July 2020

AbstractTo verify reliability of antibody detection and investigate population immunity to severe acute respiratory syndrome‐related coronavirus 2 (SARS‐CoV‐2) in the local Chinese population. A cross‐sectional study was conducted in Shenzhen to detect anti‐coronavirus antibodies including, immunoglobulin G (IgG), immunoglobulin M (IgM), and immunoglobulin A (IgA). In the COVID‐19 group, nine patients were enrolled after diagnosis. In the control group, 1589 individuals without clinical symptoms (cough, fever, and fatigue) and returning from outside Shenzhen were enrolled. The first study enrollment occurred at the end of February 2020; the final study visit was 18 March 2020. In the COVID‐19 group, the seven of nine patients were positive for IgM, IgG, and IgA. Meanwhile, six of the 1589 healthy individuals were found to be weakly positive for IgG. According to SARS‐CoV‐2 nucleic acid tests, the six individuals were all negative. Strong supplemental support for clinical information can be provided by antibody detection, especially for IgA. According to comparison with overseas reports, the infection rate of the Chinese population outside Shenzhen, China, is significantly low, so most of the population in China is still susceptible. Hence, social distancing measures are still inevitable until a vaccine is developed successfully.
Evaluation and ensemble projection of extreme high and low temperature events in China from four dynamical downscaling simulations
Authors: Guo Donglin , Zhang Ying , Gao Xuejie , Pepin Nick , Sun Jianqi 
Published: July 2020

AbstractExtreme climate events are often of great interest because of their close relationship with meteorological and hydrological disasters. In this study, four RCM simulations driven by different GCMs (CSIRO‐Mk3.6.0, EC‐EARTH, HadGEM2‐ES, and MPI‐ESM‐MR) are used as an ensemble to project changes in extreme high and low temperatures in China as represented by selected Expert Team on Climate Change Detection indices. Each simulation is performed at 25 km resolution from 1980 to 2099 using RegCM4.4 RCM. Results show the following: (a) All four reasonably reproduce climatological maximum daily maximum temperature (TX), warm spell duration, minimum daily minimum temperature (TN), and cold spell duration, but the majority shows relatively weak ability to capture climatological warm day and cold night frequencies. (b) Although the RCM homogenizes much of the variance originally shown between driving GCM simulations, marked dissimilarities are still shown in western China across the four downscaling simulations, implying a dependency on the individual GCM‐driven RCM simulation for this region. (c) All of China shows an increase in maximum TX, warm days, warm spell duration, minimum TN, and a decrease in cold nights and cold spell duration, during the near‐term, middle‐term, and long‐term. The Tibetan Plateau and its adjacent regions show more significant changes in extreme temperature, indicating stronger sensitivity to changes in extremes. RCM downscaling projects less significant change in extreme temperatures than the driving GCM. (d) The ensemble of RCM simulations projects that area‐averaged maximum TX, warm days, warm spell duration, and minimum TN increase over China as a whole by 2.5°C, 21.1%, 43.6 days, and 3.3°C, respectively, while cold nights and cold spell duration decrease by 6.8% and 2.0 days, respectively during the long‐term under the RCP4.5 scenario relative to 1986–2005. Future episodes of extreme high temperature are likely to require more research as they become more common.
New insights into the world's longest series of monthly snowfall (Parma, Northern Italy, 1777–2018)
Authors: Diodato Nazzareno , Bertolin Chiara , Bellocchi Gianni , de Ferri Lavinia , Fantini Paolo 
Published: July 2020

AbstractThe emergence of decreasing trends in snowfall frequency and snow depth highlights the challenges arising from shifts in snow regimes. In particular, snow‐dependent southern Europe regions may be negatively impacted by such changes. Snow regimes strongly influence the water availability in reservoirs and groundwater. This study presents the world's longest series of monthly snowfall (fresh snow depth, snow days per year, days with snow on the ground) for the Parma Observatory, northern Italy (44°48′N and 10°19′E) between 1777 and 2018. These datasets were collected over the centuries using early rain gauges and recently, SIAP tipping bucket models. Sources of inhomogeneity were identified and analysed through the study of metadata and of nonparametric tests on datasets. Homogenized time series were obtained after correcting the observed snowfall data for the instruments response and localization characteristics, for the observing practices with the help of the standard normal homogeneity tests. The Buishand and the Mann–Kendall tests were further applied to check the correction and detect possible monotonic trends. Over the study period, days per year of snow decreased after the change point detected in 1897, in association with a rise in surface air temperature (including a distinct urban warming trend). In this study high numbers of snowy days per year (hereafter referred as snowfall frequency) and snow depth values during the latest phase of the LIA at Parma are consistent with a cycle of minimum solar activity, suggesting that enhanced, solar‐induced blocking activity dominated, with the arrival into the Mediterranean of large cold air masses developing over Siberia and northern Europe. Over the last century, snow appears to have resonance‐like characteristics as similar trends were observed over the Northern Hemisphere, where the extent of the snow cover has been reported to markedly decline in the transition spring and autumn seasons.
Spatio‐temporal patterns of rainfall trends and the 2015–2017 drought over the winter rainfall region of South Africa
Authors: Wolski Piotr , Conradie Stefaan , Jack Christopher , Tadross Mark 
Published: July 2020

AbstractWe analyse long‐term (1900–2017) rainfall data in the southern part of the winter rainfall region of southern Africa to understand the spatial patterns of recent and long‐term trends and contextualize the 2015–2017 rainfall anomalies which led to the so‐called “Day Zero” drought in Cape Town. Our analyses reveal cohesive spatial patterns and seasonal differences in rainfall trends across a range of timescales. These suggest that rainfall is subject to regional driving mechanisms, predominantly manifested at the 20–50 year timescale, but the influence of these mechanisms is modified by subregional and seasonally specific processes, frequently resulting in trends of different magnitudes and even sign. Trend patterns are consistent with multidecadal‐scale quasi‐periodicity, with only the most recent phase (post‐1981 drying) corresponding to the expected regional response to hemispheric processes linked to anthropogenic climate change. The spatial and seasonal patterns of drying observed since 1981 alone do not explain the pattern of 2015–2017 drought anomalies, although they share a strong autumn and weak mid‐winter signal. These results have implications to the interpretation of drought in the context of observed rainfall trends. Furthermore, we identify directions for improvement of the conceptual understanding of drivers of rainfall variability and the role of anthropogenic climate change in the winter rainfall region of South Africa.
Does the order of total hip replacement and lumbar spinal stenosis surgery influence patient‐reported outcomes: An observational register study
Authors: Eneqvist Ted , Bülow Erik , Nemes Szilárd , Brisby Helena , Fritzell Peter , Rolfson Ola 
Published: July 2020

AbstractPatients with degenerative hip and lumbar spine disorders requiring surgery in both locations is fairly common in clinical practice. We investigated if the order of total hip replacement (THR) and lumbar spinal stenosis surgery (LSSS) influences patient‐reported outcomes (PROs). We used data from the Swedish Hip Arthroplasty Register (SHAR) and the Swedish Spine Register (Swespine), on patients operated with THR and LSSS in years 2002 to 2012. To increase the probability of having symptomatic disorders in both locations at the time of the first surgery, we only included patients with both LSSS and THR performed within 2 years. Linear and logistic regression analyses adjusted for age, sex, preoperative PROs, and time between surgeries were used to investigate the association between order of surgeries and the generic PRO measurements EQ‐5D and EQ VAS. Eighty‐four patients had THR prior to LSSS and 171 patients LSSS prior to THR. Linear regression showed that LSSS prior to THR was associated with higher EQ‐5D index (B = 0.09, 95% confidence interval [CI] 0.03‐0.16) and EQ VAS (B = 5.6, 95% CI 0.4‐10.9) 1 year after the last surgery. Logistic regression showed that the odds ratio [OR] for not having any problems in the “pain” (OR = 3.0, 95% CI 1.5‐6.3) and “anxiety/depression” (OR = 2.3, 95% CI 1.3‐4.1) dimensions were higher for LSSS before THR. In our cohort, LSSS before THR was associated with better health‐related quality of life outcomes compared to the reverse order. The results from our cohort can be helpful in a clinical situation where the physician gives advice to an individual patient when choosing the order of procedures. However, further studies are necessary in order to confirm these results in other cohorts. At present, standard of care remains that order of surgery should be individualized for each patient, with guidance from the operating surgeons.