bluuebunny/crossref_metadata_2025_split huggingface dataset:
Psychological Distress in Nepalese Residents during COVID-19 Pandemic: A Community Level Survey
Authors: Shrestha Dhan Bahadur , Thapa Bikash Bikram , Katuwal Nagendra , Shrestha Bikal , Pant Chiran , Basnet Bina , Mandal Pankaj , Gurung Amol , Agrawal Ankita , Rouniyar Ramhari 
Published: August 2020

Abstract
Background: COVID-19 pandemic has created unprecedented health and economic impact. Psychological stress, anxiety and depression are affecting not only COVID-19 patients but also health professionals, and general population. Restrictive measures; fear of contracting COVID-19; hardship to carry out livelihood is causing traumas to mental health. Nepal is a developing country from South Asia where the COVID-19 pandemic is yet to reach at peak. This online survey has been carried out to understand impact of COVID 19 on mental health of Nepalese community dwellers.Methods: The COVID-19 Peritraumatic Distress Index (CPDI) questionnaire adapted from the Shanghai Mental Health Centre was used for collecting data in this online survey. Data were collected from 11 April-17 May 2020. Collected data extracted to Microsoft excel-13 and imported and analyzed using SPSS (Statistical Package for Social Sciences) version-22. An initial univariate analysis was conducted for all variables to assess the distribution and logistic regression analyses were done to estimate the odds ratios of relevant predicting variables.Results: A total of 410 participants completed the whole self-rated questionnaires. Mean age of study participants was 34.8 (± 11.7) with male preponderance. Overall 88.5% of the respondents were not in distress (score less than 28) while, 11% had mild to moderate distress and 0.5% had severely distress. The prevalence of distress is higher among age > 45 years age group, female gender, and post-secondary educated. Health professional were more likely to get distressed. Respondents with post-secondary education higher odds (OR = 3.32 (p = 0.020) of developing distress as compared to respondents with secondary education or lower.Conclusion: There is lower rate of psychological distress in city dwellers and people with low education. Adequate and timely focus should be directed to mental health awareness, psychosocial support with extra focus on vulnerable groups like health care workers, female and elderly individuals.
Integrative analysis of mRNA and miRNA proﬁles identifies miR-193 and miR-210 as potential regulatory biomarkers in diﬀerent molecular subtypes of breast cancer
Authors: Evangelista Adriane Feijo , Oliveira Renato J , Silva Viviane A O , Vieira Rene A D C , Reis Rui M , Marques Marcia M C 
Published: August 2020

Abstract
Introduction: Breast cancer is the most frequently diagnosed malignancy among women. However, the role of microRNA expression in breast cancer progression is not fully understood. In this study we examined predictive interactions between differentially expressed miRNAs and mRNAs in breast cancer cell lines representative of the common molecular subtypes. Integrative bioinformatics analysis identified miR-193 and miR-210 as potential regulatory biomarkers of mRNA in breast cancer. Several recent studies have investigated these miRNAs in a broad range of tumors, but the mechanism of their involvement in cancer progression has not previously been investigated. Methods: The miRNA-mRNA interactions in breast cancer cell lines were identified by parallel expression analysis and miRNA target prediction programs. The expression profiles of mRNA and miRNAs from luminal (MCF-7, MCF-7/AZ and T47D), HER2 (BT20 and SK-BR3) and triple negative subtypes (Hs578T e MDA-MB-231) could be clearly separated by unsupervised analysis using HB4A cell line as a control. Breast cancer miRNA data from TCGA patients were grouped according to molecular subtypes and then used to validate these findings. Expression of miR-193 and miR-210 was investigated by miRNA transient silencing assays using the MCF7, BT20 and MDA-MB-231 cell lines. Functional studies included, xCELLigence system, ApoTox-Glo triplex, flow cytometry and transwell assays were performed to determine cell proliferation, cytotoxicity, apoptosis, migration and invasion, respectively. Results: The most evident effects were associated with cell proliferation after miR-210 silencing in triple negative subtype cell line MDA-MB-231. Using in silico prediction algorithms, TNFRSF10 was identified as one of the potential downstream targets for both miRNAs. The TNFRSF10C and TNFRSF10D mRNA expression inversely correlated with the expression levels of miR-193 and miR210 in breast cell lines and breast cancer patients, respectively. Other potential regulated genes whose expression also inversely correlated with both miRNAs were CCND1, a mediator on invasion and metastasis, and the tumor suppressor gene RUNX3. Conclusion: In summary, our findings identify miR-193 and miR-210 as potential regulatory miRNA in different molecular subtypes of breast cancer and suggest that miR-210 may have specific role in MDA-MB-231 proliferation. Our results highlight important new downstream regulated targets that may serve as promising therapeutic pathways for aggressive breast cancers.
The Food, Feelings, and Family Study: Comparison of the efficacy of traditional methods, social media, and broadcast email to recruit pregnant women to an observational, longitudinal nutrition study.
Authors: Smith Rebecca , Alvarez Crystal , Crixell Sylvia , Lane Michelle A 
Published: August 2020

Abstract
Background: It is well known that recruitment is a challenging aspect of any study involving human subjects. This challenge is exacerbated when the population sought is reticent to participate in research as is the case with pregnant women and individuals with depression. This paper compares recruitment methods used for the Food, Feelings, and Family Study, an observational, longitudinal study concerning how diet and bisphenol A exposure affect maternal mood and cognitive function during and after pregnancy.Methods: Pregnant women were recruited to this study using traditional methods, social media including paid and unpaid posts, and emails broadcast to the university community. Contingency analysis using the Pearson’s Chi-square test was used to determine if recruitment method was associated with likelihood of participation. T-tests were used to analyze Facebook advertisement success. ANOVAs and contingency analysis using the maximum likelihood ratio Chi-square test were used to determine if recruitment method was related to continuous and categorical demographics, respectively.Results: Social media resulted in the largest number of recruits, followed by traditional methods and broadcast email. Women recruited through social media were less likely to participate, in part, because they were less likely to meet eligibility criteria. In contrast, use of broadcast email resulted in a smaller pool of recruits but these recruits were more likely to be eligible for and complete the study. Most women recruited via social media were the result of unpaid posts to the study’s Facebook page. Paid posts lasting at least 4 days were the most successful. Recruitment method was not associated with participant demographics.Conclusions: Social media has the potential to recruit a large pool of potential subjects; however, when studies require a large time investment such as the case here, women recruited through social media are less likely to participate and complete the study than women recruited through other means.Trial registration: N/A. This study does not describe a health care intervention.
The Analysis of Harmful Factors Affecting on Mental Health and Cognitive Function Among Workers of Steel Industry (Using the ISO9612 Approach)
Authors: alimoradi Hadi , Madvari Rohollah Fallah , Nazari Mahsa , sakhvidi Mohammad javad zare , Nodoushan Reza Jafari 
Published: August 2020

Abstract
Background: In most industrial environments, workers are exposed to noise on a daily basis. The present study dealt with a set of cognitive factors to evaluate the negative emotional states in depression, anxiety, and stress in a noisy work environment. Methods: The research was a case -study that examined in 1000 male workers of steel industry. The sampling method was random sampling. The workers filled out questionnaire about, Depression, Anxiety, Stress (DASS), Cognitive Processing Inventory (CPI), and personality type tests in the study, and the ISO 9612:2009 standards was used to obtain external noise. Data was collected and analyzed using SPSS version 22 and exact test with 0.05 significant levels. Results: According to the results, age showed a significant effect on depression, marital status on anxiety, and the shift on employee stress (p <0.05). Conclusion: Finally one can conclude that given the positive and significant relationship between noise pressure level and cognitive and subjective components in the case group, it is necessary to take effective preventive measures to prevent psychological damage and to maintain workers' health in the industry. Trial registration: There is no clinical trials code in this article and only have an ethical code.
Effects of the COVID-19 Pandemic on Medical Students: A Multicenter Quantitative Study
Authors: Harries Aaron , Lee Carmen , Jones Lee , Rodriguez Robert , Davis John , Boysen-Osborn Megan , Kashima Kathleen , Krane N. Kevin , Rae Guenevere , Kman Nicholas , Langsfeld Jodi , Juarez Marianne 
Published: August 2020

Abstract
Background: The COVID-19 pandemic disrupted the United States (US) medical education system with the necessary, yet unprecedented Association of American Medical Colleges (AAMC) national recommendation to pause all student clinical rotations with in-person patient care. This study is a quantitative analysis investigating the educational and psychological effects of the pandemic on US medical students and their reactions to the AAMC recommendation in order to inform medical education policy.Methods: The authors sent a cross-sectional survey via email to medical students in their clinical training years at six medical schools during the initial peak phase of the COVID-19 pandemic. Survey questions aimed to evaluate students' perceptions of COVID-19's impact on medical education; ethical obligations during a pandemic; infection risk; anxiety and burnout; willingness and needed preparations to return to clinical rotations.Results: 741 (29.5%) students responded. Nearly all students (93.7%) were not involved in clinical rotations with in-person patient contact at the time the study was conducted, as per national recommendations. Reactions to being removed were mixed, with 75.8% feeling this was appropriate, 34.7% guilty, 33.5% disappointed, and 27.0% relieved. Most students (74.7%) agreed the pandemic had significantly disrupted their medical education, and believed they should continue with normal clinical rotations during this pandemic (61.3%). When asked if they would accept the risk of infection with COVID-19 if they returned to the clinical setting, 83.4% agreed. Students reported the pandemic had moderate effects on their stress and anxiety levels with 84.1% of respondents feeling at least somewhat anxious. Adequate personal protective equipment (PPE) (53.5%) was the most important factor to feel safe returning to clinical rotations, followed by adequate testing for infection (19.3%) and antibody testing (16.2%).Conclusions: The COVID-19 pandemic disrupted the education of US medical students in their clinical training years. The majority of students wanted to return to clinical rotations and were willing to accept the risk of COVID-19 infection. Students were most concerned with having enough PPE if allowed to return to clinical activities.
Identification of Key Genes and Long Non-Coding RNA Related to Bone Metastasis in Breast Cancer by Bioinformatics Analysis
Authors: Teng Xu , Huang Wei , Yang Tianshu , Li Weishi , Zhou Lin , Wang Zihang , Feng Yajuan , Zhang Jingyao , Yin Xin , Wang Pei , Li Gen , Yu Hefen , Chen Zhongqiang , Fan Dongwei 
Published: August 2020

Abstract
Background: The molecular mechanism of bone metastasis in breast cancer is largely unknown. We aimed to find key genes and long noncoding RNAs (lncRNAs) related to the bone metastasis of breast cancer using a bioinformatic approach. Methods: We downloaded breast cancer bone metastasis gene expression data (GSE66206) and probe annotation files from the public database Gene Expression Omnibus. Then, we analyzed the network nodes to find core driver genes. Weighted correlation network analysis was performed on the gene expression profile of breast cancer bone metastasis to identify potential pathogenic modules. We performed function and pathway enrichment analyses of the genes and lncRNAs in the breast cancer bone metastasis-related modules. Results: Based on our results, we constructed a differentially expressed lncRNA–mRNA network related to bone metastases in breast cancer and identified core driver genes, including BNIP3 and RP11-317-J19.1. Conclusions: Our findings provide a theoretical foundation for explaining the intrinsic molecular mechanism of breast cancer bone metastasis.
Why Market Orientation Matters for Agriculture and Fishery Workers? Unravelling the Association between Occupational Background and Caloric Deprivation in India
Authors: Kumar Smita , Rajpal Sunil , Lu Shiau-Yun , Joe William 
Published: August 2020

Abstract
Background: Given the presumptions of low-productivity, developmental policies in low- and middle-income countries usually display a pro-manufacturing bias and largely undermine the potentials within the agriculture sectors in promoting sustainable growth and development. As a consequence, skill development is usually aligned with preferences of the manufacturing-led economic growth and is accorded high priorities in policymaking. However, the success of such skill development policies essentially hinges upon the assumption of a quick and successful structural transformation towards non-agricultural sector (manufacturing and services). But contrary to expectations, the pace of economic transition has been rather slow in India and almost one-half of the workforces continue to be engaged in agriculture and allied activities. Moreover, it is unclear whether such shift away from agriculture can necessarily lead to reductions in widespread deprivations in the form of food insecurity and under nutrition. In fact, it is also feasible that market orientation of skilled agricultural and fishery sector may display favorable impact on poverty and nutritional well-being of households. Methods: Therefore, drawing upon data from 68th round (2011-12) of nationally representative cross-sectional Household Consumer Expenditure Survey (HCES) of National Sample Survey (NSS), Government of India, this study aims to examine the association between occupational backgrounds and nutritional deprivation (average caloric consumption as well as low calorie intake) among Indian households. Results: Evidences show that agricultural and fishery labor households have lowest calorie intake (2086 kcal) across all the occupational groups. However, market oriented skilled agricultural and fishery workers’ (2261 kcal – rural, 2165 kcal - urban) have higher calorie intakes than those belonging to subsistence agricultural (2165 kcal – rural, 2149 kcal - urban) and fishery workers or agricultural and fishery laborer (2086 kcal – rural, 2071 kcal - urban). Further, the multilevel logistic regression estimates suggest that in rural areas, households engaged in skilled agricultural and fishery works have significantly (at 5% level) lower odds ratio (OR: 0.72, with 95% CI: 0.63; 0.82) of having insufficient calorie intake compared to the unskilled agricultural and fishery laborer households. Conclusion: These insights when combined with the occupation-specific random-effects suggests that policy focus to promote market oriented skilled agricultural and fishery workers can be an equally good option as direct investment in professional and managerial skills for manufacturing and services.
Global Transcriptome Changes of Elongating Internode of Sugarcane in Response to Mepiquat Chloride
Authors: Chen Rongfa , Fan Yegeng , Zhou Huiwen , Mo Shanping , Zhou Zhongfeng , Yan Haifeng , Luo Ting , Huang Xing , Weng Mengling , Lakshmanan Prakash , Li Yangrui , Qiu Lihang , Wu Jianming 
Published: August 2020

Abstract
Background: Mepiquat chloride (DPC) is an extensively used chemical to control internode growth and compact canopies in cultured plants. Previous studies suggested that DPC could inhibit gibberellin biosynthesis in sugarcane. Unfortunately, the molecular mechanism underlying the suppressive effects of DPC on plant growth is still largely unknown. Results: In the present study, we first obtained the high-quality long transcripts from internode of sugarcane by PacBio Sequel System. A total of 72,671 isoforms with N50 as 3,073 were generated. These long isoforms were used for the following RNA-seq as reference. Subsequently, short reads generated from Illumina Hiseq 4000 platform were used for comparing the differentially expressed genes in DPC and control groups. The transcriptome profiling showed the 6 days post DPC treatment had the most significant changed genes. These genes were related to plant hormone signal transduction and biosynthesis of several metabolites, indicating DPC affected multiple pathways beside depressed gibberellin biosynthesis. The network of DPC on the key stage was illustrated by weighted gene co-expression network analysis (WGCNA). Among the constructed 36 modules, the top positive correlated module with stage of 6 day post spraying DPC was sienna3. Stf0 sulphotransferase, cyclin-like F-box and HOX12 were the hub genes in sienna3 that had high correlation with other genes in this module. The qPCR demonstrated the high accuracy of RNA-seq result. Conclusion: Taken together, we demonstrated the key role of these genes in DPC-induced growth inhibition in sugarcane.
Emergent spin-liquid-like state in the bond-disordered breathing pyrochlore
Authors: Lee Suheon , Do Seunghwan , Lee W.-J. , Choi Y.-S. , Tol Johan Van , Reyes Arneil , Skourski Yurii , Chen Wei-tin , Choi K.-Y. 
Published: August 2020

Abstract
A breathing pyrochlore system is predicted to host a variety of quantum spin liquids. However, perturbations beyond nearest-neighbor Heisenberg interaction are an obstacle to identifying such exotic states. Here, we utilize a bond-alternating disorder to tune a magnetic ground state in the Cr-based breathing pyrochlore. By combining thermodynamic and magnetic resonance techniques, we provide experimental signatures of a spin-liquid-like state in LiGa1-xInxCr4O8 (x=0.2), namely, a nearly T2-dependent magnetic specific heat and a persistent spin dynamics by muon spin relaxation (μSR). Moreover, 7Li NMR, ZF-μSR, and ESR unveil the dichotomic nature of both temporal and thermal spin fluctuations: slowly fluctuating tetramer singlets at high temperatures and a fast fluctuating spin-liquid-like state at low temperatures. Our results suggest that a bond disorder in the breathing pyrochlore offers a new route to achieve an unexplored state of matter.
Angiotensin-Converting Enzyme Inhibitors (ACEI) or Angiotensin Receptor Blockers (ARBs) May Be Safe for COVID-19 Patients
Authors: Wang Wenjun , Zhao Xiaohui , Wei Wei , Fan Weiwang , Gao Kai , He Shengxiu , Zhuang Xijing 
Published: August 2020

Abstract
Background: To investigate the effects of angiotensin-converting enzyme inhibitor (ACEI) and angiotensin receptor blockers (ARBs) administration to hypertension patients with the coronavirus disease 2019 (COVID-19) induced pneumonia.Methods: We recorded the recovery status of 67 inpatients with hypertension and COVID-19 induced pneumonia in the Leishenshan Hospital in Wuhan during February 12, 2020 and March 30, 2020. Patients treated with ACEI or ARBs were categorized in group A (n = 22), while patients who were not administered either ACEI or ARBs were categorized into group B (n = 45). We did a comparative analysis of various parameters such as the pneumonia progression, length-of-stay in the hospital, and the level of alanine aminotransferase (ALT), serum creatinine (Cr), and creatine kinase (CK) between the day when these patients were admitted to the hospital and the day when the treatment ended.Results: These 67 hypertension cases counted for 33.17% of the total COVID-19 patients. There was no significant difference in the usage of drug treatment of COVID-19 between groups A and B (p > 0.05). During the treatment, 1 case in group A and 3 cases in group B progressed from mild pneumonia into severe pneumonia. Eventually, all patients were cured and discharged after treatment, and no recurrence of COVID-2019 induced pneumonia occurred after the discharge. The length of stays was shorter in group A as compared with group B, but there was no significant difference (p > 0.05). There was also no significant difference in other general parameters between the patients of the groups A and B on the day of admission to the hospital (p > 0.05). The ALT, CK, and Cr levels did not significantly differ between groups A and B on the day of admission and the day of discharge (p > 0.05). Conclusions: To treat the hypertension patients with COVID-19 caused pneumonia, anti-hypertensive drugs (ACEs and ARBs) may be used according to the relative guidelines. The treatment regimen with these drugs does not need to be altered for the COVID-19 patients.
Dynamic methylation of histone H3K18 in differentiating Theileria parasites
Authors: Cheeseman Kevin , Jannot Guillaume , Lourenco Nelly , Villares Marie , BERTHELET Jeremy , Calegari-Silva Teresa , Hamroune Juliette , Letourneur Franck , Rodrigues-Lima Fernando , Weitzman Jonathan 
Published: August 2020

Abstract
Lysine methylation on histone tails impacts genome regulation and cell fate determination in many developmental processes. Apicomplexa intracellular parasites cause major diseases and they have developed complex life cycles with fine-tuned differentiation events. Yet, apicomplexa genomes have few transcription factors and little is known about their epigenetic control systems. Tick-borne Theileria apicomplexa species have relatively small compact genomes and a remarkable ability to transform leukocytes in their bovine hosts. Here we report enriched H3 lysine 18 monomethylation (H3K18me1) on the gene bodies of repressed genes in Theileria macroschizonts. Differentiation to merozoites (merogony) led to decreased H3K18me1 in parasite nuclei. Pharmacological manipulation of H3K18 acetylation or methylation impacted parasite differentiation and expression of stage-specific genes. Finally, we identified a parasite SET-domain methyltransferase (TaSETup1) that can methylate H3K18 and represses gene expression. Thus, H3K18me1 emerges as an important epigenetic mark which controls gene expression and stage differentiation in Theileria parasites.