bluuebunny/crossref_metadata_2025_split huggingface dataset:
Measuring the Concentration of Protein Nanoparticles Synthesized by Desolvation Method: Comparison of Bradford Assay, BCA Assay, hydrolysis/UV Spectroscopy and Gravimetric Analysis
Authors: Khramtsov Pavel , Kalashnikova Tatyana , Bochkova Maria , Kropaneva Maria , Timganova Valeria , Zamorina Svetlana A. , Rayev Mikhail B. 
Published: November 2020

Research paper on sunthesis of protein nanoparticlesAbstractThe desolvation technique is one of the most popular methods for preparing protein nanoparticles for medicine, biotechnology, and food applications. We fabricated 11 batches of BSA nanoparticles and 2 batches of gelatin nanoparticles by desolvation method. BSA nanoparticles from 2 batches were cross-linked by heating at +70 °C for 2 h; other nanoparticles were stabilized by glutaraldehyde. We compared several analytical approaches to measuring their concentration: gravimetric analysis, bicinchoninic acid assay, Bradford assay, and alkaline hydrolysis combined with UV spectroscopy. We revealed that the cross-linking degree and method of cross-linking affect both Bradford and BCA assay. Direct measurement of protein concentration in the suspension of purified nanoparticles by dye-binding assays can lead to significant (up to 50-60%) underestimation of nanoparticle concentration. Quantification of non-desolvated protein (indirect method) is affected by the presence of small nanoparticles in supernatants and can be inaccurate when the yield of desolvation is low. The reaction of cross-linker with protein changes UV absorbance of the latter. Therefore pure protein solution is an inappropriate calibrator when applying UV spectroscopy for the determination of nanoparticle concentration. Our recommendation is to determine the concentration of protein nanoparticles by at least two different methods, including gravimetric analysis.
Surto de raiva em morcegos frugívoros no município de Niterói, RJ, 2018
Authors: Moutinho Flavio Fernando Batista , Nunes Viviane Moura Azevedo , Fernandes Paulo Mafra , Borges Fábio Villas Boas , Faria Neto Francisco De 
Published: November 2020

A raiva é uma doença viral gravíssima que pode afetar todos os mamíferos. Trata-se de um estudo exploratório descritivo documental desenvolvido a partir dos dados do Centro de Controle de Zoonoses do município de Niterói, RJ. No mês de julho de 2018 ocorreram três casos de raiva em morcegos, diagnosticados por imunofluorescência direta e inoculação em camundongos, todos envolvendo Artibeus sp. e localizados numa pequena área do bairro São Francisco. O Centro de Controle de Zoonoses desenvolveu uma série de ações visando impedir a transmissão da doença para humanos e outras espécies de animais. Os resultados sugerem um possível surto de raiva em morcegos no bairro São Francisco, em julho de 2018. A ocorrência de um evento desse em um bairro da área urbana de uma metrópole pode ser considerado um fator preocupante pela possibilidade de ocorrência de casos da doença em humanos ou animais domésticos, principalmente tendo em vista a existência dos demais fatores condicionantes e determinantes da ocorrência da enfermidade encontrados no município. Nesse sentido, esforços devem ser envidados pelo poder público no sentido de eliminar ou mitigar tais fatores, visando salvaguardar a saúde da população.
Criptococose cutânea canina: relato de caso
Authors: Herculano Lícia Flávia Silva , Galindo Victor Reis , Cavalcante Neto Tobias Saraiva , Santos Lúcia De Fátima Lopes dos 
Published: November 2020

Criptococose é uma doença multissistêmica que acomete humanos e animais. É transmitida pela inalação de esporos de um fungo da espécie Cryptococcus neoformans que se desenvolve em matéria orgânica, principalmente em fezes de aves e madeira em decomposição. Esta enfermidade é, em geral, de caráter oportunista sendo mais comum em gatos do que em cães. As manifestações clínicas dessa doença variam e podem causar distúrbios oftálmicos, respiratórios, cutâneos e neurológicos. O diagnóstico vai além da avaliação física e anamnese, podendo o médico veterinário solicitar exames micológicos (cultura fúngica e esfregaço de material da lesão), citológicos, histopatológicos, entre outros para especificar qual realmente é a causa do problema e tratar de modo mais adequado possível. O tratamento é baseado no uso de antifúngicos sistêmicos, no entanto, a literatura não relata o tempo mínimo adequado de tratamento, indicando manter o protocolo terapêutico por mais 30 ou 60 dias após melhora do quadro clínico e resultados de exames negativos para criptococose. O presente trabalho tem o objetivo de relatar um caso de criptococose cutânea em uma cadela de nove anos da raça Pastor Alemão. A paciente apresentava lesões ulcerativas no membro torácico esquerdo, que causavam dor quando palpadas. O diagnóstico foi confirmado por cultura fúngica e a terapia foi realizada com itraconazol na dose de 10mg/kg, via oral (VO), a cada 24h (q24h) inicialmente, fazendo ajustes posteriores para 20mg/kg VO, q24h totalizando cinco meses de terapia, resultando em melhora da doença e sem efeitos adversos, para a paciente relatada. Assim, é necessário que exames complementares e diagnósticos mais precisos sejam realizados para contribuírem para uma terapia adequada objetivando a cura clínica.
Pengembangan Modul Pembelajaran Kimia Berbasis Augmented Reality
Authors: Ramadani Reski , Ramlawati Ramlawati , Arsyad Muhammad 
Published: November 2020

The study is research and development that aims to develop augmented reality-based chemistry learning module which is valid, practical, and effective. Augmented reality-based chemistry learning module refers to the 4-D development model by Thiagarajan et. al., which includes the stages of defining, designing, developing and disseminating. This learning modules had been conducted the validation stage by the assessors or experts and gone through field trial, revised by the developer to produce learning module which is valid, effective, and practical. The results of the study reveal that: (1) the assessments of feasibility or validity of the media expert and a material expert are in the very valid category; (2) in the field trial stage to 33 students of grade XI MIA 1 at SMAN 10 Makassar obtained the mean score of 80.00 learning outcomes from an ideal score of 100 with a grade completeness of 82%; (3) the responses of students and teachers respectively with a percentage of 80.38% and 100%, so the module is stated as practical. Therefore, the conclusion of the study is the augmented reality-based chemistry learning module which had been developed using 4D model is valid, practical, and effective to be applied in learning.
Comparison of the Accuracy of Item Response Theory Models in Estimating Studentâ€™s Ability
Authors: Falani Ilham , Akbar Makruf , Naga Dali Santun 
Published: November 2020

This study aims to determine the item response theory model which is more accurate in estimating students' mathematical abilities. The models compared in this study are Multiple Choice Model and Three-Parameter Logistic Model. Data used in this study are the responses of a mathematical test of 1704 eighth-grade junior high school students from six schools in the Depok City, West Java. The Sampling is done by using a purposive random sampling technique. The mathematics test used for research data collection consisted of 30 multiple choice format items. After the data is obtained, Research hypotheses were tested using the variance test method (F-test) to find out which model is more accurate in estimating ability parameters. The results showed that Fvalue is obtained 1.089, andÂ Ftable is 1.087, the value of Fvalue > Ftable, so it concluded that Ho rejected. That means Multiple Choice Model is more accurate than Three-Parameter Logistic Model in estimating the parameters of students' mathematical abilities. This makes the Multiple-Choice Model a recommended model for estimating mathematical ability in MC item format tests, especially in the field of mathematics and other fields that have similar characteristics.
Distributed Practice Learning Model Using Audiovisual Media for Teaching Basic Skills of Badminton
Authors: Kamaruddin Ilham , Nur Muhammad , Sufitriyono Sufitriyono 
Published: November 2020

This study investigated the effectiveness of implementation of distributed practice learning model by audio visual media to improve the results of learning basic techniques of badminton through. This research was a experimental methods one group Pretest-posttest design. The research object is students a 4th semester in grade 2018, amounting to 40 people obtained through purposive sampling techniques. Samples were given treatment for 8 times the meeting, in a week 3 times meeting.Â Data collection techniques of badminton skills test in the form of Wall Volley test, short service, long service and clear test. Data analysis techniques using T-test.Â The results of this study were a significant effects on the implementation of the distributed practice learning model using audio visual media to improve the basic technical skills of badminton students.Â The audio-visual media in the application of distributed practice learning models is very effective in improving the learning outcomes of badminton basic engineering skills.Â Besides being able to facilitate students in understanding the movements learned, also facilitate teachers in delivering learning materials taught at the time, because students can evaluate the movement directly to be perfect through video observation.
ANALISIS SPASIAL TINGKAT KEKERINGAN SEBAGAI UPAYA MITIGASI KEGAGALAN PANEN DI KABUPATEN TAKALAR SULAWESI SELATAN
Authors: Badwi Nasiah , Maru Rosmini , Abbas Ibrahim 
Published: November 2020

Penelitian ini bertujuan untuk menggambarkan karakteristik curah hujan, evapotransirasi, agihan tingkat kekeringan, dan upaya mitigasi kegagalan panen di Kabupaten Takalar Penelitian ini dilakukan dengan metode neraca air (Water Balance) oleh Thornthwaite – Mather (1957), dengan kriteria indeks kekeringan ILACO, B.V. (1981). Hasil penelitian menunjukkan karakterisitk curah hujan bulanan di wilayah Kabupaten Takalar berkisar antara 9,5 mm hingga 835,5 mm. Curah hujan bulanan terbesar di stasiun Bonto Mangape dan terkecil di stasiun Palomo Selatan. Curah hujan tahunan berkisar antara 2282, 8 mm hingga 3546,69 mm/tahun. Karakteristik Evapotranspirasi potensial bulanan di wilayah Kabupaten Takalar berkisar antara 112,89 mm hingga 170,76 mm. Evapotranspirasi potensial rerata tahunan sebesar 1428,76 mm di Stasiun Palomo Selatan hingga 1576,28 mm di Stasiun Patalassang. Tingkat kekeringan tinggi dan merata di seluruh wilayah Kabupaten Takalar yaitu pada bulan Juli. Agihan tingkat kekeringan tahunan tinggi tersebar di wilayah timur ke selatan yaitu; Kecamatan Polongbangken Utara, Polongbangken Selatan dan Mangngarabombang. Mitigasi kegagalan panen dengan cara mengelola tanaman sesuai dengan ketersediaan air. Pada tingkat keketingan tinggi ditanam tanaman Semangka, Mentimun dan jagung.
POTENSI NANOPARTIKEL ALGINAT- KITOSAN - EKSTRAK DAUN KEDONDONG HUTAN (SPONDIAS PINNATA (L.f.) Kurz.) DALAM PENATALAKSANAAN TUBERKULOSIS DAN MULTI DRUG RESISTANCE TUBERCULOSIS (MDR-TB)
Authors: Gitari Ni Made , Bagus I Gusti Ngurah , Putra Ida bagus Nyoman 
Published: November 2020

Tuberkulosis (TB) merupakan penyakit infeksi akibat M.tuberculosis yang mengakibatkan morbiditas dan mortalitas yang tinggi. Merebaknya strain M. tuberculoisis yang resisten terhadap obat anti tuberkulosis membuat penanganan TB menjadi semakin sulit dan beban ekonomi yang tinggi. Tujuan dari penulisan paper gagasan tulis ini untuk mendeskripsikan potensi nanopartikel alginat-kitosan-ekstrak daun kedondong hutan sebagai penatalaksanaan TB dan MDR-TB. Dari 87 jurnal yang ditelaah, 50 jurnal ditemukan sesuai dengan topik bahasan dan digunakan sebagai referensi karya ini. Kombinasi penyalutan alginat-kitosan merupakan kombinasi yang paling tepat karena dapat meningkatkan bioavailabilitas dan pelepasan berkelanjutan dari obat antibakteri. Nanopartikel alginat-kitosan-ekstrak daun kedondong hutan dapat menghambat sintesis asam mikolat M. tuberculosis, proteasom dan mengakibatkan lisis dinding sel M. tuberculosis. Ekstrak daun kedondong hutan mengandung senyawa flavonoid dan triterpenoid yang berfungsi sebagai antituberkulosis. Flavonoid dapat berikatan dengan situs aktif HadB sehingga menghambat aktivitas enzim β-hydroxyacyl-ACP dehydratase yang berfungsi dalam elongasi rantai meromycolic. Selain itu, flavonoid dapat menghambat proteasom M. tuberculosis yang dalam keadaan dorman. Triterpenoid memiliki gugus sehingga mampu mengakibatkan lisis dinding sel M. tuberculsosis. Nanopartikel alginat-kitosan-ekstrak daun kedondong hutan memiliki potensi sebagai terapi baru untuk TB dan MDR-TB. Belum terdapat penelitian mengenai kombinasi modalitas ini sehingga diperlukan penelitian lebih lanjut untuk mengetahui potensi pasti nanopartikel alginat-kitosan-ekstrak daun kedondong hutan.
Model Regresi Spasial dan Aplikasinya pada Kasus Tingkat Kemiskinan Kabupaten Soppeng
Authors: Putri Anggi Ananda , Sanusi Wahidah , Sukarna Sukarna 
Published: November 2020

Poverty is one of the major problem that frequently faced by human. Begin from poverty, consequently emerged several social issues, such as homeless, beggar, defendant, and prostitution. On this research were conducted modeling poverty degree in Soppeng with using number of poor household as the dependent variable. Modeling were done by using area approach which is a Spatial Autoregressive (SAR) model and Spatial Error Model (SEM). As for the independent variable used on this research is the number of health services, school facility, population density, social well being disable, and the distance on village and centre of Soppeng. Regarding to the analysis of Spatial Autoregressive (SAR) and Spatial Error Model (SEM) shows that there is a spatial dependency lag and error on number of poor household variable. As for the independent variable which have the significancy account for 5% on Spatial Autoregressive (SAR) and Spatial Error Model (SEM) are every variables with a number R2= 90,9% on SAR and R2= 90,1% on SEM.
Association between Oral Error Corrections of University Teacher and English Majorsâ€™ Language Anxiety in Philippine Higher Education Context
Authors: Guibangguibang Herford Rei Biscayno 
Published: November 2020

This study sought to answer the question on the level of English language anxiety in the ESL and mainstream classrooms of 61 total number of English major students utilizing the 20-item self-assessment questionnaire of English Language Anxiety Scale (ELAS) developed by Pappamihiel (2002). Actual one-hour footage of classroom was documented through a MONACORR audio-recorder, thrice with each teacher. The recorder was given to one of the teachersâ€™ students without knowledge for the authenticity of their oral corrections. After having identified the existing oral error corrections by the English teachers through audio-recording, a self-made questionnaire was answered by the students to find what is the rate of occurrence of the identified oral error correction styles. The frequency count, weighted mean, and Chi-square tests were the statistical tools used to answer the problems posed in this study. Findings divulged after the transcription, teachers were only utilizing elicitation, explicit correction, recast, and repetition. Moreover, students perceived that elicitation oral error correction type is often used while explicit correction, recast, and repetition are only used sometimes by their English teachers.Â Meanwhile, ELAS results indicate levels of language anxiety in the ESL classes and mainstream, although language anxiety is significantly higher in ESL classes. As to correlation, it was found out that the rate of occurrence of explicit correction, recast, and repetition as perceived by ESL English major students of their English teachersâ€™ oral error correction types have no significant relationships to their English language anxiety. This study suggests that oral error correction has nothing to do with the English language anxiety of English major students and that English teachers shall retain the practice of the oral error correction for it does not give high level of learnersâ€™ anxiety in learning English.Â
Residents Social Behavior in The Implementation of Denpasar City Waste Management Policy
Authors: Elele Emmanuel Chinedu , Subanda I Nyoman 
Published: November 2020

With the increasing level of waste generation in Denpasar city reaching above 1000 tons per day in the midst of rapid population growth and a surge in tourism activities, residents’ adherence to waste management procedures constitutes a vital component in the government policy strategy towards tackling waste management challenges related to health , hygiene and environmental sustainability. This study aims to ascertain and analyze, implementation of Denpasar City waste management policy, to ascertain and analyze the social behavior of Denpasar city residents in the implementation of waste management policy, and to find out and analyze factors influencing the implementation of Denpasar city waste management policy. Data were obtained through in-depth interviews with research informants selected through purposive sampling technique, observation, and review of documents. Triangulation method was used for data validation while Miles-Huberman's interactive data analysis model was used analysis. Conclusion drawn from this research is that residents' parochial and non-compliance behavior to waste management standard operational procedures hinder effective implementation of waste management policy in Denpasar city..
The Effectiveness of Interpersonal Communication on Employees at Vocational High School YPLP PGRI 1 Makassar
Authors: Sazwani Nurul , Jamaluddin Jamaluddin , Niswaty Risma 
Published: November 2020

As human social beings in everyday life always want to relate to other humans. He wants to know the surrounding environment, even wants to know what is happening in him. This curiosity forces humans to communicate. This study aims to determine the effectiveness of interpersonal communication among employees at Vocational High School YPLP PGRI 1 Makassar. Therefore, to achieve these objectives researchers used data collection techniques with observation, questionnaires, interviews, and documentation with a population of 24 people. And the data is processed using the quantitative descriptive analysis to determine the extent of the effectiveness of interpersonal communication among employees at the Vocational High School YPLP PGRI 1 Makassar. The results of this study indicate that the Effectiveness of Interpersonal Communication among Vocational High School YPLP PGRI 1 Makassar staff with indicators namely: openness with a percentage reaching 90.83 percent, empathy with a percentage reaching 90.56 percent, supportiveness with a percentage of 83.13 percent, positiveness with a percentage reaching 89.44 percent and equality with a percentage reaching 92.08 percent of all the indicators are in the very good category. This is demonstrated through overall data analysis which shows the percentage reached 89.08 percent, the value is in line with related observations made by researchers in the field.
KEMAMPUAN MENDESAIN MOTIF BATIK MAHASISWA PROGRAM STUDI PENDIDIKAN SENI RUPA JURUSAN SENI RUPA FAKULTAS SENI DAN DESAIN UNIVERSITAS NEGERI MAKASSAR
Authors: MUSTAKIM NUR JANNAH , Hasnawati Hasnawati , Mallabasa Yabu 
Published: November 2020

Penelitian ini bertujuan untuk memperoleh data yang akurat mengenai kemampuan Mahasiswa Angkatan 2017 Program Studi Pendidikan Seni Rupa Fakultas Seni dan Desain Univesitas Negeri Makassar dalam Mendesain Motif Batik. Jenis penelitian ini adalah penelitian evaluatif pada taraf deskriptif kuantitatif yang menjelaskan mengenai kemampuan mahasiswa dalam mendesain motif batik. Variabel dalam penelitian ini adalah kemampuan mahasiswa pendidikan seni rupa dalam mendesain motif batik. Sumber data diperoleh dari Dosen dan Mahasiswa. Populasi pada penelitian ini sebanyak 2 kelas sebanyak 69 orang mahasiswa. Seluruh populasi dijadikan sampel dalam penelitian ini, sehingga teknik pengambilan sampel adalah sampel total. Teknik pengumpulan data digunakan dalam penelitian ini yaitu, observasi, dan dokumentasi. Hasil penelitian menunjukkan bahwa kemampuan mahasiswa angkatan 2017 Program Studi Pendidikan Seni Rupa Fakultas Seni dan Desain Univesitas Negeri Makassar dalam Mendesain Motif Batik termasuk dalam kategori cukup.
Appropriating the Ethos of Confucius for the Rebuilding of the Nigerian Commonwealth
Authors: Udoh Emmanuel Williams 
Published: November 2020

The Nigerian commonwealth has been bastardized by our national political and economic managers. This has given rise to the current cry in search of unity, peace and patriotism of Nigerians. It can be said again that things have fallen apart and the centre seems not to hold any longer. The current wave of corruption, bloodshed, ethnic militia and communal and political subdivisions and carpet crossing at our political arena are clear signs of the deteriorating state of our Nigerian commonwealth. Good Nigerians desire to see Nigeria return back to her former glory of oneness, prosperity and pride. That is why this research explored the ethos of Confucius for the rebuilding of the Nigerian commonwealth. This work which is anchored on the sociological theory of “Structural Functionalism” propounded by Emile Durkheim (1850-1917) and adopted the qualitative or exploratory research method in gathering information. It employed the content analysis approach in examining available printed materials on the subject matter. The findings from the research showed that Nigerians have deviated from the ethical values that had initially been the source of strength and toe the path of selfishness, ethnicity and division. This new path became a path to corruption and disintegration. The research recommends an inclusiveness of human responsibility for improving life; adoption of an ideal political system founded on “ideals of personal conduct” rather than on formerly enacted laws; and adoption of a viable religious ethos that will revitalize the ailing economic, social and political status of the Nigerian commonwealth.
Hubungan Sosial Kognitif dan Life Skills Education terhadap Kemampuan Adaptasi Calon Guru pada Era Revolusi Industri 4.0 Mahasiswa S1 PTE Jurusan Teknik Elektro Universitas Negeri Malang
Authors: Wistiawati Artina Tri , Putro Setiadi Cahyono , Irianto Wahyu Sakti Gunawan 
Published: November 2020

Era revolusi industri 4.0 menuntut calon guru untuk dapat beradaptasi dengan baik (Y) karena terjadinya arus perubahan jaman terutama untuk calon guru SMK. Oleh sebab itu Mahasiswa sebagai calon guru harus memiliki Life Skills Education (X2) dalam pelatihan softskill maupun hardskill. Selain itu dalam beradaptasi calon guru (Y) diperlukan kemampupan berbaur dengan orang lain atau sosial kognitif (X1) sehingga adaptasi dapat dilakukan dengan baik. Penelitian ini bertujuan untuk mengetahui : (1) Deskripsi X1, X2, Y, (2) Hubungan X1 dengan Y, (3) Hubungan X2 dengan Y, (4) Hubungan X1 dan X2 dengan Y. Metode penelitian yang digunakan yaitu kuantitatif dengan rancangan penelitian deskriptif korelasional. Sampel penelitian sebanyak 84 siswa yang ditentukan dengan berpedoman pada teknik sampel jenuh. Variabel X1, X2 dan Y memiliki tingkat reliabilitas secara berurutan adalah 0.973, 0.985. dan 0.985. Secara Berurutan Nilai Sumbangan Efektif pada X1 dan X2 adalah 19.68% dan 50.05%. Secara Berurutan Nilai Sumbangan Relatif pada X1 dan X2 adalah 28.20% dan 71.80%. Kesimpulan hasil yang diperoleh dari penelitian ini yaitu (1) X1,X2, dan Y dalam kategori sedang; (2) terdapat hubungan positif dan signifikan antara X1 dan Y, X2 dan Y, dan X1 dan X2 secara simultan dengan Y.