bluuebunny/crossref_metadata_2025_split huggingface dataset:
Three multi-allelic gene pairs are responsible for self-sterility in the ascidian Ciona intestinalis
Authors: Sawada Hitoshi , Yamamoto Kazunori , Yamaguchi Akira , Yamada Lixy , Higuchi Arata , Nukaya Haruhiko , Fukuoka Masashi , Sakuma Tetsushi , Yamamoto Takashi , Sasakura Yasunori , Shirae-Kurabayashi Maki 
Published: February 2020

AbstractMany hermaphroditic organisms possess a self-incompatibility system to avoid inbreeding. Although the mechanisms of self-incompatibility in flowering plants are well known, little is known about the mechanisms of self-sterility in hermaphroditic marine invertebrates. Ascidians are hermaphroditic sessile marine invertebrates that release sperm and eggs into the surrounding seawater. Several species, includingCiona intestinalistype A(Ciona robusta), exhibit strict self-sterility. In a previous study, we found that the candidate genes responsible for self-sterility inCionareside in chromosome 2q (locus A) and chromosome 7q (locus B). Two pairs of multi-allelic genes, nameds(sperm)-Themis-Aandv(vitelline-coat)-Themis-Ain locus A ands-Themis-Bandv-Themis-Bin locus B, are responsible for self-sterility. In this study, we identified a third multi-allelic gene pair,s-Themis-B2andv-Themis-B2, within locus B that is also involved in this system. Genetic analysis revealed that the haplotypes ofs/v-Themis-A, s/v-Themis-Bands/v-Themis-B2play essential roles in self-sterility. When three haplotypes were matched betweens-Themisandv-Themis, fertilization never occurred even in nonself crossing. Interestingly, gene targeting of eithers/v-Themis-B/B2ors/v-Themis-Aby genome editing enabled self-fertilization. These results indicate thats/v-Themis-A, -Band-B2areS-determinant genes responsible for self-sterility in the ascidianC. intestinalistype A.
Distinct pressure half-time values by transthoracic echocardiography for grading of paravalvular regurgitation after transcatheter aortic valve replacement
Authors: Schröder Joerg , Almalla Mohammad , Saad Mohammed , Mezger Matthias , Keszei Andras , Frick Michael , Lotfi Shahram , Hoffmann Rainer , Becker Michael , Altiok Ertunc 
Published: February 2020

AbstractPostprocedural aortic regurgitation (AR) has negative impact on patient outcome after transcatheter aortic valve replacement (TAVR). Standard assessment of AR severity by echocardiography is hampered after TAVR. Measurement of pressure half-time (PHT) by echocardiography is not limited in these patients but it may be affected by concomitant left ventricular hypertrophy (LVH). This study sought to evaluate distinct cut-off values of PHT differentiating between patients without and with more than mild LVH for grading of AR after TAVR with cardiac magnetic resonance (CMR) as the reference method for comparison. 71 patients (age 81 ± 6 years) with severe aortic stenosis undergoing TAVR were included into the study. Transthoracic echocardiography (TTE) and CMR were performed after TAVR. Left ventricular mass index was calculated by TTE. PHT was measured by continuous-wave Doppler echocardiography of aortic regurgitation jet. In 18 patients (25%) PHT could not be obtained due to no or very faint Doppler signal. Aortic regurgitant volume and regurgitant fraction were calculated by CMR by flow analysis of the ascending aorta. In 14 of 53 patients (26%) AR after TAVR was moderate or severe as categorized by CMR analysis. More than mild LVH was present in 27 of 53 patients (51%). PHT correlated inversely less to regurgitant fraction by CMR analysis in patients with LVH (r = −0.293; p = 0.138) than in patients without LVH (r = −0.455; p = 0.020). In patients without relevant LVH accuracy of PHT to predict moderate or severe paravalvular regurgitation AUC was 0.813 using a cut-off value of 347 ms and AUC was 0.729 in patients with more than mild LVH using a cut-off value of 420 ms. Analysis of PHT by TTE with distinct cut-off values for patients without and with more than mild LVH allows detection of moderate or severe AR after TAVR as defined by CMR. In none of the patients in which PHT could not be measured AR was categorized as more than trace by CMR analysis.
Superparamagnetic state in La0.7Sr0.3MnO3 thin films obtained by rf-sputtering
Authors: Ramírez Camacho M. C. , Sánchez Valdés C. F. , Curiel M. , Sánchez Llamazares J. L. , Siqueiros J. M. , Raymond Herrera O. 
Published: February 2020

AbstractA novel superparamagnetic state has been observed in high quality La0.7Sr0.3MnO3 (LSMO) thin films directly grown by rf-sputtering on SiOx/Si(100) substrates. The films are nanostructured without grain boundaries, constituted by locally epitaxial nanoregions grown layer-by-layer with out-of-plane (012) preferential orientation, induced by the constrain of the native silicon oxide. Low magnetic field ZFC-FC magnetization curves show a cross-over from superparamagnetic to ferromagnetic state dependent of the thickness. The thicker film (140 nm) exhibits typical ferromagnetic order. The thinner films (40 and 60 nm) exhibit superparamagnetic behavior attributed to interacting ferromagnetic monodomain nanoregions with critical size, random in-plane oriented, where the inter-monodomain boundaries with surface spin-glass structure regulate the blocking of magnetization depending on the magnetic field intensity. M(H) hysteresis loops showed noticeable coercive fields in all samples, larger than those reported for LSMO. Such properties of half-metal LSMO film foresee potential integration in new Si-technology nanodevices in Spintronics.
Full annual monitoring of Subantarctic Emiliania huxleyi populations reveals highly calcified morphotypes in high-CO2 winter conditions
Authors: Rigual-Hernández A. S. , Trull T. W. , Flores J. A. , Nodder S. D. , Eriksen R. , Davies D. M. , Hallegraeff G. M. , Sierro F. J. , Patil S. M. , Cortina A. , Ballegeer A. M. , Northcote L. C. , Abrantes F. , Rufino M. M. 
Published: February 2020

AbstractOcean acidification is expected to have detrimental consequences for the most abundant calcifying phytoplankton speciesEmiliania huxleyi. However, this assumption is mainly based on laboratory manipulations that are unable to reproduce the complexity of natural ecosystems. Here,E. huxleyicoccolith assemblages collected over a year by an autonomous water sampler and sediment traps in the Subantarctic Zone were analysed. The combination of taxonomic and morphometric analyses together within situmeasurements of surface-water properties allowed us to monitor, with unprecedented detail, the seasonal cycle ofE. huxleyiat two Subantarctic stations.E. huxleyisubantarctic assemblages were composed of a mixture of, at least, four different morphotypes. Heavier morphotypes exhibited their maximum relative abundances during winter, coinciding with peak annual TCO2and nutrient concentrations, while lighter morphotypes dominated during summer, coinciding with lowest TCO2and nutrients levels. The similar seasonality observed in both time-series suggests that it may be a circumpolar feature of the Subantarctic zone. Our results challenge the view that ocean acidification will necessarily lead to a replacement of heavily-calcified coccolithophores by lightly-calcified ones in subpolar ecosystems, and emphasize the need to consider the cumulative effect of multiple stressors on the probable succession of morphotypes.
Radiogenomics predicts the expression of microRNA-1246 in the serum of esophageal cancer patients
Authors: Hoshino Isamu , Yokota Hajime , Ishige Fumitaka , Iwatate Yosuke , Takeshita Nobuyoshi , Nagase Hiroki , Uno Takashi , Matsubara Hisahiro 
Published: February 2020

AbstractRadiogenomics is a new field that provides clinically useful prognostic predictions by linking molecular characteristics such as the genetic aberrations of malignant tumors with medical images. The abnormal expression of serum microRNA-1246 (miR-1246) has been reported as a prognostic factor of esophageal squamous cell carcinoma (ESCC). To evaluate the power of the miR-1246 level predicted with radiogenomics techniques as a predictor of the prognosis of ESCC patients. The real miR-1246 expression (miR-1246real) was measured in 92 ESCC patients. Forty-five image features (IFs) were extracted from tumor regions on contrast-enhanced computed tomography. A prediction model for miR-1246real was constructed using linear regression with selected features identified in a correlation analysis of miR-1246real and each IF. A threshold to divide the patients into two groups was defined according to a receiver operating characteristic analysis for miR-1246real. Survival analyses were performed between two groups. Six IFs were correlated with miR-1246real and were included in the prediction model. The survival curves of high and low groups of miR-1246real and miR-1246pred showed significant differences (p = 0.001 and 0.016). Both miR-1246real and miR-1246pred were independent predictors of overall survival (p = 0.030 and 0.035). miR-1246pred produced by radiogenomics had similar power to miR-1246real for predicting the prognosis of ESCC.
Mysterious long-living ultrahigh-pressure or secondary impact crisis
Authors: Shumilova T. G. , Zubov A. A. , Isaenko S. I. , Karateev I. A. , Vasiliev A. L. 
Published: February 2020

AbstractHigh-pressure glass has attracted interest in terms of both its fundamental state under extreme conditions and its possible applications as an advanced material. In this context, natural impact glasses are of considerable interest because they are formed under ultrahigh-pressure and high-temperature (UHPHT) conditions in larger volumes than laboratory fabrication can produce. Studying the UHPHT glasses of the unique giant Kara astrobleme (Russia), we found that the specific geological position of the UHPHT melt glass veins points to an origin from a secondary ultrahigh-pressure (UHP) melt according to the characteristics of the host suevites, which suggest later bottom flow. Here, we propose a fundamentally novel model involving an upward-injected UHP melt complex with complicated multi-level and multi-process differentiation based on observations of the UHP silica glass, single-crystal coesite and related UHP smectite that crystallized from an impact-generated hydrous melt. This model proposes a secondary UHP crisis during the modification stage of the Kara crater formation. The results are very important for addressing fundamental problems in fields as diverse as condensed matter states under extreme pressure and temperature (PT) conditions, material and geological reconstructions of impact structures, water conditions in mineral substances under UHP conditions in the deep Earth, and the duration and magnitude of the catastrophic effects of large asteroid impacts.
Chronic circadian misalignment accelerates immune senescence and abbreviates lifespan in mice
Authors: Inokawa Hitoshi , Umemura Yasuhiro , Shimba Akihiro , Kawakami Eiryo , Koike Nobuya , Tsuchiya Yoshiki , Ohashi Munehiro , Minami Yoichi , Cui Guangwei , Asahi Takuma , Ono Ryutaro , Sasawaki Yuh , Konishi Eiichi , Yoo Seung-Hee , Chen Zheng , Teramukai Satoshi , Ikuta Koichi , Yagita Kazuhiro 
Published: February 2020

AbstractModern society characterized by a 24/7 lifestyle leads to misalignment between environmental cycles and endogenous circadian rhythms. Persisting circadian misalignment leads to deleterious effects on health and healthspan. However, the underlying mechanism remains not fully understood. Here, we subjected adult, wild-type mice to distinct chronic jet-lag paradigms, which showed that long-term circadian misalignment induced significant early mortality. Non-biased RNA sequencing analysis using liver and kidney showed marked activation of gene regulatory pathways associated with the immune system and immune disease in both organs. In accordance, we observed enhanced steatohepatitis with infiltration of inflammatory cells. The investigation of senescence-associated immune cell subsets from the spleens and mesenteric lymph nodes revealed an increase in PD-1+CD44high CD4 T cells as well as CD95+GL7+ germinal center B cells, indicating that the long-term circadian misalignment exacerbates immune senescence and consequent chronic inflammation. Our results underscore immune homeostasis as a pivotal interventional target against clock-related disorders.
Butterfly-shaped magnetoresistance in triangular-lattice antiferromagnet Ag2CrO2
Authors: Taniguchi Hiroki , Watanabe Mori , Tokuda Masashi , Suzuki Shota , Imada Eria , Ibe Takashi , Arakawa Tomonori , Yoshida Hiroyuki , Ishizuka Hiroaki , Kobayashi Kensuke , Niimi Yasuhiro 
Published: February 2020

AbstractSpintronic devices using antiferromagnets (AFMs) are promising candidates for future applications. Recently, many interesting physical properties have been reported with AFM-based devices. Here we report a butterfly-shaped magnetoresistance (MR) in a micrometer-sized triangular-lattice antiferromagnet Ag2CrO2. The material consists of two-dimensional triangular-lattice CrO2 layers with antiferromagnetically coupled S = 3/2 spins and Ag2 layers with high electrical conductivity. The butterfly-shaped MR appears only when the magnetic field is applied perpendicularly to the CrO2 plane with the maximum MR ratio (≈15%) at the magnetic ordering temperature. These features are distinct from those observed in conventional magnetic materials. We propose a theoretical model where fluctuations of partially disordered spins with the Ising anisotropy play an essential role in the butterfly-shaped MR in Ag2CrO2.
Promoter polymorphisms in the lncRNA-MIAT gene associated with acute myocardial infarction in Chinese Han population: a case–control study
Authors: Ma Ruchao , He Xiaohui , Zhu Xiaoyun , Pang Shuchao , Yan Bo 
Published: February 2020

Abstract
Background: Coronary atherosclerotic disease (CAD) is one of the greatest causes of death and disability around the world, and has emerged as a major public health problem. Acute myocardial infarction (AMI) is the most serious type of CAD. Myocardial infarction (MI) association transcript (MIAT) has demonstrated that it plays an important role in AMI. Purpose: To investigate the association between MIAT promoter polymorphisms and AMI in Chinese Han population. Methods: A total of 212 AMI patients and 218 healthy controls were recruited. The long non-coding RNA (lncRNA)-MIAT promoter polymorphisms (single nucleotide polymorphisms (SNPs)) were obtained using polymerase chain reaction (PCR) and sequencing techniques. Chi-square test was used to analyze the allele and genotype frequencies of each SNP in two groups. Logistic regression analysis was used to analyze the association of each SNP with AMI. Linkage disequilibrium (LD) and haplotype analysis were performed using SHEsis software. A JASPAR database search predicts transcription factors transition of linked polymorphism in MIAT promoter. Results: Ten SNPs were found, including rs56371714, rs55892869, rs151057042, rs2157598, rs150465374, rs5761664, rs8142890, rs5752375, rs9608515 and rs1055293700, whereas rs1055293700 was found only in the control group. Single and logistic regression analysis showed that there was a significant correlation between rs5752375 and rs9608515 polymorphisms and AMI, while other sites had no relationship with AMI. These MI association polymorphisms may change the binding sites with transcription factor. Conclusions: The polymorphisms of lncRNA-MIAT promoter rs5752375 and rs9608515 were significantly associated with AMI in Chinese Han population. This result would be of clinical importance for the early diagnosis of AMI.
180 GHz high‐gain cascode power amplifier in a 130 nm SiGe process
Authors: Li Xingcun , Chen Wenhua , Wang Yunfan , Feng Zhenghe 
Published: February 2020

A positive feedback gain‐enhanced technique for power amplifiers has been introduced in this Letter. With the proposed circuit design method for optimal inductive feedback, a 180 GHz high‐gain single‐stage cascode power amplifier in a 130 nm SiGe process is implemented. The measurement results show a power gain of 10 dB and 20 GHz bandwidth with 110 mW dc power are achieved, and 6.5 dBm of output power referred to P1dB and 3.5% collector efficiency are attained. The chip area of the power amplifier is only 0.50 × 0.48 mm2.
Anämien und Erythrozytentransfusionen in der Neugeborenenperiode
Authors: Maier Rolf F. , Franz Axel R. 
Published: February 2020

ZusammenfassungIn kaum einer anderen Periode des Lebens gibt es so viele verschiedene Ursachen für eine Anämie wie in der Perinatalzeit. Neugeborene und insbesondere Frühgeborene mit ihrem geringen Blutvolumen zählen zu den Patientengruppen mit den meisten Erythrozytentransfusionen. Dieser Beitrag informiert über Besonderheiten bei der Indikation für und der Durchführung von Erythrozytentransfusionen in der Neonatalperiode sowie über Maßnahmen zu deren Vermeidung.
Phytoestrogens in NAFLD: Potential Mechanisms of Action
Authors: Li Hui , Jia Erna , Jiao Jian 
Published: February 2020

AbstractMany studies have shown that estrogen has a protective effect on premenopausal
women with metabolic disorders and non-alcoholic fatty liver disease. Estrogen
supplements may, at least in theory, prevent the development and progression of
NAFLD, while the possibility of inducing cancer limits its application in
practice. Phytoestrogen is extracted from plants, whose molecular structure and
biological activity are similar to those of mammals' estrogen,
therefore, could replace the role of estrogen and prevent the occurrence of
adverse reactions to estrogen. This article reviews the published literature
related to phytoestrogens and NAFLD as well as suggest the possible mechanisms
that may underlie the association between phytoestrogens and NAFLD. It is hoped
to provide basis for the treatment of NAFLD with phytoestrogen.
Interaction between Solvatochromic Dyes and Water Sampled from a Natural Source Treated with High Dilutions of Phosphorus
Authors: Aparicio Ana Carla C. , de Oliveira Larissa Helen S. , Silva Jefferson S. , Coelho Cideli P. , Pinheiro Sonia Regina , Souza Monica F. , Suffredini Ivana B. , Cartwright Steven J. , Bonamin Leoni Villano 
Published: February 2020

Abstract
Background Highly diluted and succussed solutions interact with solvatochromic dyes, indicating that changes in solvent and solute polarity could be related to their mechanism of action. It is not known, however, how the activity associated with succussed high dilutions is transferred to untreated water and what the limits of this process are.
Aims The aims of the present study were to ascertain whether a succussed high dilution of phosphorus (1.5 × 1−59 M; Phos 30cH) seeded into a natural water source that fed a fjord and two connected lakes could propagate itself through the lake system (total volume 2200 m3) and, moreover, whether the process could be tracked using solvatochromic dyes.
Methods Samples of water were collected before and after seeding, at different times and places throughout the lake system. Controls comprised water taken from an untreated and adjacent, but independent, lake (1385 m3).
Results Water samples taken up to 72 hours after the source treatment produced significant increases (p ≤ 0.03) in the absorbance of the solvatochromic dye methylene violet (MV), while samples from the control lake produced no changes.
Conclusions The study indicates that activity associated with Phos 30c can propagate itself through large volumes of water, causing changes throughout a whole connected lake system, and that these changes can be tracked using the solvatochromic dye MV. This in turn means the use of homeopathic medicines in large volumes of drinking water, in farming and ecological contexts, now has the potential to be assessed with physico-chemical monitoring.
Orientation of Cone-Beam Computed Tomography Image: Pursuit of Perfect Orientation Plane in Three Dimensions—A Retrospective Cross-Sectional Study
Authors: Balachandran Rajiv , Kharbanda Om Prakash , Sennimalai Karthik , Neelapu Bala Chakravarthy 
Published: February 2020

Abstract
Objective This study aimed to evaluate the reproducibility of nine reference planes used in orientation of as-received cone-beam computed tomography (CBCT) images in all three dimensions.
Materials and Methods The study was conducted on CBCT images of 15 adult subjects (mean age 21.2 ± 5.8 years). The anonymized CBCT images were oriented using five different methods created from nine reference planes by two experienced orthodontists. For each subject, pitch, yaw, and roll changes with five orientation methods were recorded twice by each observer.
Statistical Analysis The inter- and intraobserver agreement was tested using intraclass correlation (ICC) and Bland–Altman plot. The intra- and interobserver error was analyzed using paired t-test. Analysis of variance and paired t-test were used to analyze the differences among the various pitch, roll, and yaw orientation planes.
Results Inter- and intraobserver agreement (ICC, 0.9) was excellent for all the nine reference planes. The interobserver reliability showed statistically significant differences for four planes namely Frankfort horizontal plane constructed on right side (p = 0.014) and left side (p = 0.000), transorbital plane (p = 0.001), and midsagittal plane on top view (p = 0.036); however, the mean differences were clinically insignificant.
Conclusion The landmark-based nine reference planes used in this study to orient CBCT images showed good reproducibility. Therefore, these reference planes can be used to orient CBCT images and can be incorporated into automated software.
Stereoselective Analysis of Methadone and EDDP in Laboring Women and Neonates in Plasma and Dried Blood Spots and Association with Neonatal Abstinence Syndrome
Authors: Metzger Ingrid F. , Thomas Anna E. , Evrard Cindy A. , Jones David R. , Masters Andrea R. , Haas David M. , Haneline Laura S. , Quinney Sara K. 
Published: February 2020

Abstract
Objective This pilot study evaluated the relationship between maternal and neonatal R- and S-methadone and R- and S-2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP) exposure and the severity of neonatal abstinence syndrome (NAS). The use of dried blood spots (DBS) as an alternative for plasma in assessing methadone and EDDP was also assessed.
Study Design Women receiving methadone for medication assisted treatment of opioid use disorder during pregnancy were eligible for recruitment. Plasma and DBS samples were collected from mothers during labor, from cord blood, and from newborns during genetic screen. R-/S-methadone and EDDP were measured by high-performance liquid chromatography tandem mass spectrometry (HPLC/MS/MS). Associations between methadone exposure, neonatal morphine requirements, and severity of NAS were examined.
Results Twenty women and infants completed the study. Maternal methadone dose at delivery was 112 mg/day (range = 60–180 mg/day). Sixteen neonates experienced NAS requiring morphine; three also required phenobarbital. Higher cord blood concentrations of R-methadone, R- and S-EDDP were associated with higher maximum doses of morphine (p < 0.05).
Conclusion Maternal methadone and cord blood concentration at delivery are variable and may be potential markers of neonatal abstinence syndrome.
Lymph Node Involvement in T1a Glottic Carcinoma: Controversial Case Series
Authors: Yalcinozan Eda Tuna , Safak Mustafa Asim 
Published: February 2020

AbstractThe treatment modality of laryngeal cancer is complete resection of the primary lesion and dissection of the cervical lymph nodes that is related to their staging. Following the patient without neck dissection is the usual expectation in T1 glottic carcinomas. However, a combination of elective neck dissection with surgical treatment for clinically N0 neck in early glottic tumors may be an effective treatment modality in the prevention of missed micrometastases. In these two case reports, elective neck dissection and re-examination of the treatment were discussed accordingly. We aimed to point out the importance of evaluation, examination, and, if it is uncertain, moreover the dissection of lymph nodes in early-stage glottic carcinoma since there are rarely any studies available.