bluuebunny/crossref_metadata_2025_split huggingface dataset:
GM2 ganglioside accumulation causes neuroinflammation and behavioral alterations in a mouse model of early onset Tay-Sachs disease
Authors: Demir Seçil Akyıldız , Timur Zehra Kevser , Ateş Nurselin , Martínez Luis Alarcón , Seyrantepe Volkan 
Published: September 2020

Abstract
Background
Tay-Sachs disease (TSD), a type of GM2-gangliosidosis, is a progressive neurodegenerative lysosomal storage disorder caused by mutations in the α subunit of the lysosomal β-hexosaminidase enzyme. This disease is characterized by excessive accumulation of GM2 ganglioside, predominantly in the central nervous system. Although Tay-Sachs patients appear normal at birth, the progressive accumulation of undegraded GM2 gangliosides in neurons leads to death. Recently, an early onset Tay-Sachs disease mouse model, with genotype Hexa−/−Neu3−/−, was generated. Progressive accumulation of GM2 led to premature death of the double KO mice. Importantly, this double-deficient mouse model displays typical features of Tay-Sachs patients, such as cytoplasmic vacuolization of nerve cells, deterioration of Purkinje cells, neuronal death, deceleration in movement, ataxia, and tremors. GM2-gangliosidosis is characterized by acute neurodegeneration preceded by activated microglia expansion, macrophage, and astrocyte activation, along with the production of inflammatory mediators. However, the mechanism of disease progression in Hexa−/−Neu3−/− mice, relevant to neuroinflammation is poorly understood.

Method
In this study, we investigated the onset and progression of neuroinflammatory changes in the cortex, cerebellum, and retina of Hexa−/−Neu3−/− mice and control littermates by using a combination of molecular genetics and immunochemical procedures.

Results
We found elevated levels of pro-inflammatory cytokine and chemokine transcripts, such as Ccl2, Ccl3, Ccl4, and Cxcl10 and also extensive microglial and astrocyte activation and proliferation, accompanied by peripheral blood mononuclear cell infiltration in the vicinity of neurons and oligodendrocytes. Behavioral tests demonstrated a high level of anxiety, and age-dependent loss in both spatial learning and fear memory in Hexa−/−Neu3−/− mice compared with that in the controls.

Conclusion
Altogether, our data suggest that Hexa−/−Neu3−/− mice display a phenotype similar to Tay-Sachs patients suffering from chronic neuroinflammation triggered by GM2 accumulation. Furthermore, our work contributes to better understanding of the neuropathology in a mouse model of early onset Tay-Sachs disease.
Durability of Structural Lightweight Concrete Containing Expanded Perlite Aggregate
Authors: Ibrahim Mohammed , Ahmad Aftab , Barry Mohammed S. , Alhems Luai M. , Mohamed Suhoothi A. C. 
Published: September 2020

AbstractThis study focuses on the development of durable structural lightweight concrete (LWC) by incorporating expanded perlite aggregate (EPA) in the range of 0 to 20% by weight. In order to ensure its durability when exposed to chloride environment, concrete was produced with low water-to-cement ratio and ordinary Portland cement (OPC) was replaced with 50% and 7% ground granulated blast furnace slag (GGBFS) and silica fume (SF), respectively. The mechanical properties and durability of concrete were assessed by determining the unit weight, compressive strength, flexural strength, drying shrinkage, chloride permeability and migration, as well as resistance of concrete to corrosion of reinforcing steel. Very importantly, thermal insulation properties were determined using a hot guarded plate. In addition, a finite element model (FEM) was prepared to study the behavior of EPA-modified concrete under seismic loading. The results showed that the unit weight of concrete was reduced by 20% to 30% when compared with the normal weight concrete (NWC). The compressive strength of the developed LWC was sufficient to be used as structural concrete, particularly of those mixtures containing 10% and 15% perlite aggregate. The durability of LWC was comparable to NWC in terms of chloride diffusion and resistance of concrete to corrosion of reinforcing steel. The tangible outcomes also include the superior thermal insulation properties of LWC compared to NWC. The greater incorporation of EPA in the concrete resulted in better behavior under seismic loading.
Verification Testing to Confirm V˙O2max in a Hot Environment
Authors: PRYOR J. LUKE , LEIJA ROBERT GARCIA , MORALES JACOBO , POTTER ADAM W. , LOONEY DAVID P. , PRYOR RIANA R. , HOSTLER DAVID , COCHRANE-SNYMAN KRISTEN C. 
Published: September 2020

ABSTRACT
Verification tests to confirm graded exercise test (GXT) V˙O2max are growing in popularity, but the validity and reliability of such testing in the heat remains unknown.

Purpose
This study aimed to evaluate the validity and reliability of a verification test to confirm GXT V˙O2max in a hot environment.

Methods
Twelve recreationally trained cyclists completed a two-test protocol that included a GXT progressing 20 W·min−1 followed by a biphasic supramaximal-load verification test (1 min at 60% increasing to 110% maximal GXT wattage until failure) in a hot environment (39°C, 32% relative humidity). Rest between tests occurred in a thermoneutral room and was anchored to the duration required for gastrointestinal temperature to return to baseline.

Results
Mean verification test V˙O2max (51.3 ± 8.8 mL·kg−1·min−1) was lower than GXT (55.9 ± 7.6 mL·kg−1·min−1, P = 0.02). Verification tests confirmed GXT V˙O2max in 92% of participants using individual analysis thresholds. Bland–Altman analysis revealed a sizable mean bias (−4.6 ± 4.9 mL·kg−1·min−1) with wide 95% limits of agreement (−14.0 to 5.0 mL·kg−1·min−1) across a range of V˙O2max values. The high coefficient of variation (9.6%) and typical error (±3.48 mL·kg−1·min−1) indicate potential issues of test–retest reliability in the heat.

Conclusions
Verification testing in a hot condition confirmed GXT V˙O2max in virtually all participants, indicating robust utility. To enhance test–retest reliability in this environment, protocol recommendations for work rate and recovery between tests are provided.
Mud Pack With Menthol and Arnica Montana Accelerates Recovery Following a High-Volume Resistance Training Session for Lower Body in Trained Men
Authors: Bartolomei Sandro , Nigro Federico , D'Amico Alessio , Cortesi Matteo , Di Michele Rocco 
Published: September 2020

Abstract
Bartolomei, S, Nigro, F, D'Amico, A, Cortesi, M, and Di Michele, R. Mud pack with menthol and Arnica montana accelerates recovery following a high-volume resistance training session for lower body in trained men. J Strength Cond Res 36(7): 1909–1915, 2022—The aim of this study was to compare the effects of a mud pack, containing menthol and Arnica montana, on the recovery responses following a high-volume resistance protocol. Ten resistance-trained men (mean ± SD: age = 25.3 ± 6.1 years; body mass = 79.1 ± 10.6 kg; height = 178.9 ± 7.5 cm) performed a high-volume resistance workout for lower body squat and leg extension, (5 sets of 10 reps at 70% of one repetition maximum for both exercises). All the subject used mud (MUD) or a placebo (PL) in randomized counterbalanced crossover design. MUD or PL were applied 4 times: 3, 19, 27, and 45 hours after the workout, on the skin surface above the quadriceps muscle of both legs. Muscle performance (countermovement jump power [CMJP], isokinetic leg press at 75 cm·s−1 and 25 cm·s−1 [ISOK75 and ISOK25, respectively], isometric squat [ISQ]), and morphology (muscle thickness of vastus lateralis [VLMT]), were measured before exercise (baseline [BL]), and 15 minutes (15P), 24 hours (24P), and 48 hours (48P) postexercise. In addition, muscle soreness was assessed at the same time points using a visual analog scale (VAS). No significant interactions (p > 0.05) between the trials were detected for CMJP, ISOK75, ISQ, and VLMT. A significant interaction between trials was noted for ISOK25 (p = 0.022) and for VAS (p = 0.001). ISOK25 was significantly (p < 0.01) reduced from BL at 15P, 24P and 48P in PL, whereas changes were significant (p < 0.05) at 15P and 24P only in MUD. The present findings indicate that MUD may enhance the recovery rate of strength and reduce muscle soreness after high-volume exercise. Muscle morphology do not seem to be influenced by mud packs.
Bodyweight BOSU Ball Exercises
Authors: Snarr Ronald Lee , Wirbiezcas Michelle , Colbert Alesondra , Langford Emily , Hogan Gina 
Published: September 2020

ABSTRACT
A growing trend in strength and conditioning is the execution of traditional exercises on instability devices. With the addition of these implements, there is a greater challenge to maintain stability and balance. Implementing a device, such as the BOSU ball, may provide benefits to individuals rehabilitating from an injury, older individuals, and those looking to increase overall health benefits. Thus, this column provides detailed descriptions of the proper technique, advanced progressions, and exercise prescription for 4 bodyweight BOSU ball exercises.
KELALAIAN MANUSIA (HUMAN ERROR) DALAM KECELAKAAN LALU LINTAS: ANALISIS BERDASARKAN PEMROSESAN INFORMASI
Authors: Guritnaningsih Guritnaningsih , Tjahjono Tri , Maulina Dewi 
Published: September 2020

Indonesia face a serious problem of high number of death caused by traffic accidents. All this time human error or human negligence are considered as the cause of traffic accidents. The aim ot this study is to analyze the factors of causes and the dynamics of the traffic accidents from the drivers’ cognitive factor. Four wheel vehicles drivers and motorcyclist who were the prepetrators of traffic accidents were interviewed. Result of this study shows that negligence in recognizing situations as well as in making decision while driving becomes dominant factors in human error. The implication of this study is to re-evaluate the policy of driving license requirements, and the socialization program about safety on the road.
Tingginya angka kematian akibat kecelakaan di jalan raya merupakan masalah serius yang dihadapi oleh Indonesia. Selama ini human error atau kelalaian manusia seringkali ditunjuk sebagai penyebab terjadinya kecelakaan lalu lintas. Penelitian ini bertujuan untuk menganalisis faktor penyebab dan dinamika terjadinya kecelakaan lalu lintas ditinjau dari faktor kognitif yang ada pada diri pengemudi/pengendara. Wawancara dilakukan terhadap pengemudi kendaraan roda empat dan pengendara sepeda motor yang berperan sebagai pelaku terjadinya kecelakaan lalu lintas. Hasil penelitian menunjukkan bahwa kelalaian dalam mengenali situasi dan kelalaian dalam membuat keputusan bertindak dalam mengemudi/mengendara menjadi faktor dominan dalam human error. Implikasi dari peneltian ini adalah dapat dikembangkannya kebijakan bagi persyaratan perolehan Surat Ijin Mengemudi dan program sosialisasi keselamatan di jalan raya.
PENENTUAN BIAYA KECELAKAAN LALU LINTAS: STUDI KASUS LOMBOK TIMUR
Authors: Kusuma Andyka , Tjahjono Tri , Achjar Nuzul 
Published: September 2020

Traffic accident cost analysis is needed to predict potential losses of nation due to the emergence of traffic accident victims. The cost of traffic accidents in Indonesia is based on the characteristics of the City of Bandung in 2003, and it is shown that the cost of traffic accidents can vary according to regional characteristics. This study tries to compare the analytical approach in Bandung in 2003 with the Consumer Price Index and Human Development Index approaches for an area with medium accessibility, namely East Lombok, West Nusa Tenggara Province. The location of traffic accidents greatly influences the cost of traffic accidents, because the costs on inter-city roads are relatively higher compared to those on roads in the city. This study shows that the cost of traffic accidents is related to accessibility of health facilities. This analysis can be developed for all regions in Indonesia, so that it can be used as a reference for traffic stakeholders in assessing the performance of the traffic safety programs implemented.
Analisis biaya kecelakaan lalu lintas diperlukan untuk memprediksi potensi kerugian negara akibat timbulnya korban kecelakaan lalu lintas. Biaya kecelakaan lalu lintas di Indonesia dibangun berdasarkan karakteristik kota Bandung tahun 2003, dan diperlihatkan bahwa biaya kecelakaan lalu lintas dapat bervariasi sesuai dengan karakteristik wilayah. Penelitian ini mencoba membandingkan pendekatan analisis di Bandung tahun 2003 dengan pendekatan Indeks Harga Konsumen dan Indeks Pembangunan Manusia untuk suatu wilayah dengan aksesibilitas menengah, yakni Lombok Timur, Provinsi Nusa Tenggara Barat. Lokasi kecelakaan lalu lintas sangat mempengaruhi biaya kecelakaan lalu lintas, karena biaya di jalan antar kota relatif lebih tinggi dibandingkan dengan biaya di jalan dalam kota. Penelitian ini meperlihatkan bahwa biaya kecelakaan lalu lintas terkait dengan aksesibilitas fasilitas kesehatan. Analisis ini dapat dikembangkan untuk semua wilayah di Indonesia, sehingga dapat dijadikan acuan bagi pemangku kepentingan lalu lintas dalam menilai kinerja program keselamatan lalu lintas yang dijalankan.
FAKTOR PENYEBAB KECELAKAAN LALU LINTAS: STUDI KASUS BEBERAPA JALAN DI KOTA SURABAYA
Authors: Widyastuti Hera , Utami Adita 
Published: September 2020

Indonesia on the fifth ranks in the world considering of accident number, after China, India, Afghanistan, and Nigeria, where East Java is the province that has the highest accident in Indonesia. The city of Surabaya as the Capital of East Java Province automatically became the centre of government activities, giving rise to transportation activities that indirectly had an impact on accidents. Therefore, it is necessary to conduct research to obtain the accident rate and the location of black site in the city of Surabaya. In addition, considering that productive age including junior high school (SMP) is the highest contributor on the accidents, the level of understanding of junior high school students on traffic regulations needs to be analysed. From the data obtained at IRSMS, it can be seen that five roads in Surabaya were the highest contributors to accidents in Surabaya, namely Mastrip, Diponegoro, A. Yani, Kenjeran, and Ir. Soekarno, where the five roads are inter-city connecting roads that blend with the road in the city. In addition, from the interview data, there was a relationship between awareness of traffic behaviour and willingness to protect themselves.
Indonesia menduduki peringkat kelima, setelah Tiongkok, India, Afghanistan, dan Nigeria. Dan Jawa Timur adalah propinsi yang mempunyai kecelakaan tertinggi di Indonesia. Kota Surabaya sebagai Ibukota Provinsi Jawa Timur secara otomatis menjadi pusat kegiatan pemerintahan, sehingga menimbulkan kegiatan transportasi yang secara tidak langsung berdampak pada terjadinya kecelakaan. Oleh karenanya perlu dilakukan penelitian untuk memperoleh nilai accident rate, serta black site di Kota Surabaya. Selain itu mengingat usia produktif termasuk anak sekolah tingakat Sekolah Menengah Atas (SMP) adalah penyumbang kecelakaan tertinggi, maka tingkat pemahaman para pelajar SMP terhadap peraturan lalu lintas perlu di analisa. Dari data yang didapat pada IRMS, terlihat bahwa lima jalan di kota Surabaya menjadi penyumbang kecelakaan tertinggi di Surabaya, yaitu jalan A. Yani, Mastrip, Diponegoro, Kenjeran dan Ir. Soekarno, dimana kelima jalan tersebut adalah jalan-jalan penghubung antar kota yang berbaur dengan jalan dalam kota. Selain itu dari data wawancara diperoleh hubungan antara niat dengan kepatuhan terhadap peraturan lalu lintas.
STUDI KOMPARASI PEMILIHAN ANGKA KECEPATAN KENDARAAN DI PURWOKERTO DAN SEMARANG
Authors: Hardini Probo , Hidayati Nurul , I Eva Wahyu 
Published: September 2020

Driver speed that exceeds the limit is one of many factors that trigger road accidents. This study aims to analyze the differences in driver perception in choosing the speed of the vehicle. This research was conducted in two cities with characteristics assumed to have different socio-cultural aspects, namely Semarang City representing the big city and Purwokerto City as the medium city. The study uses questionnaire data distributed to respondents of vehicle users and analyzed using quantitative descriptive methods. It is known that motorists or drivers in the two cities above have the same perception related to the reason for speeding, namely travel time and punctuality to the destination. However, there are differences in perceptions on the negative effect that underlie the decision making, namely self-actualization for the release of anger and praise. Referring to several reasons in the questionnaire, the answers of drivers in Semarang lead to the poles agree, while in Purwokerto, they lead to disagreeing. Also, it was found that the driver's knowledge of speed limits can affect the choice of speed values taken. This can be seen in the high percentage of respondents who do not know the speed limit of urban roads and the high percentage of motorists who do the speeding.
Kecepatan kendaraan pengendara yang melebihi batas menjadi salah satu faktor pencetus terjadinya kecelakaan di jalan raya. Penelitian ini bertujuan untuk menganalisis perbedaan persepsi masyarakat dalam berkendara. Penelitian ini dilakukan di dua kota dengan karakteristik yang diasumsikan mempunyai sosial budaya yang berbeda, yaitu Kota Semarang merepresentasikan kota besar dan Kota Purwokerto sebagai kota sedang. Penelitian ini menggunakan data kuisioner yang dibagikan kepada responden pengguna kendaraan, dan dianalisis dengan metode deskriptif kuantitatif. Berdasarkan hasil analisis dapat diketahui bahwa pengendara di kedua kota di atas mempunyai persepsi yang sama terkait dengan alasan berkendara melebihi kecepatan, yaitu waktu tempuh dan ketepatan waktu sampai di tujuan. Meskipun demikian, ada perbedaan persepsi pada pengaruh negatif yang mendasari pengambilan keputusan tersebut, yaitu aktualisasi diri untuk pelampiasan marah dan mendapat pujian. Berdasarkan beberapa alasan yang ada di kuisioner, jawaban pengendara dan pengemudi di Kota Semarang mengarah pada kutub setuju, sedangkan di Kota Purwokerto mengarah tidak setuju. Selain itu diketahui bahwa pengetahuan pengendara tentang batas kecepatan dapat mempengaruhi pemilihan nilai kecepatan yang diambil. Hal ini terlihat pada tingginya persentase responden yang tidak mengetahui batas kecepatan jalan perkotaan dan tingginya persentase pengendara yang mengemudi di atas batas kecepatan.
Developing a Process for the Analysis of User Journeys and the Prediction of Dropout in Digital Health Interventions: Machine Learning Approach
Authors: Bremer Vincent , Chow Philip I , Funk Burkhardt , Thorndike Frances P , Ritterband Lee M 
Published: September 2020

Background
User dropout is a widespread concern in the delivery and evaluation of digital (ie, web and mobile apps) health interventions. Researchers have yet to fully realize the potential of the large amount of data generated by these technology-based programs. Of particular interest is the ability to predict who will drop out of an intervention. This may be possible through the analysis of user journey data—self-reported as well as system-generated data—produced by the path (or journey) an individual takes to navigate through a digital health intervention.

Objective
The purpose of this study is to provide a step-by-step process for the analysis of user journey data and eventually to predict dropout in the context of digital health interventions. The process is applied to data from an internet-based intervention for insomnia as a way to illustrate its use. The completion of the program is contingent upon completing 7 sequential cores, which include an initial tutorial core. Dropout is defined as not completing the seventh core.

Methods
Steps of user journey analysis, including data transformation, feature engineering, and statistical model analysis and evaluation, are presented. Dropouts were predicted based on data from 151 participants from a fully automated web-based program (Sleep Healthy Using the Internet) that delivers cognitive behavioral therapy for insomnia. Logistic regression with L1 and L2 regularization, support vector machines, and boosted decision trees were used and evaluated based on their predictive performance. Relevant features from the data are reported that predict user dropout.

Results
Accuracy of predicting dropout (area under the curve [AUC] values) varied depending on the program core and the machine learning technique. After model evaluation, boosted decision trees achieved AUC values ranging between 0.6 and 0.9. Additional handcrafted features, including time to complete certain steps of the intervention, time to get out of bed, and days since the last interaction with the system, contributed to the prediction performance.

Conclusions
The results support the feasibility and potential of analyzing user journey data to predict dropout. Theory-driven handcrafted features increased the prediction performance. The ability to predict dropout at an individual level could be used to enhance decision making for researchers and clinicians as well as inform dynamic intervention regimens.
Effect of Curcuma longa Aqueous Extract on Male Fertility in Aluminum Exposed Wistar Rats
Authors: BOUDOU Farouk , BENDAHMANE-SALMI Malika , BENABDERRAHMANE Mokhtar , BELAKREDAR Amal , BENALIA Abdelkrim , BEGHDADLI Benali , KANDOUCI Bader dine Abdelkrim 
Published: September 2020

To assess the effects of Curcuma longa aqueous extract (CE) on fertility in male rats exposed to Aluminum chloride (AlCl3), Twenty four male rats, 3 months old, divided into 4 groups (n=6) were used. Animal from the first group received, by the oral route, water-containing AlCl3 at the dose of 34 mg/kg body weight (BW). Rats from the second group received both AlCl3 at the same dose in combination with oral treatment of CE at a dose of 200 mg/kg BW. The third group received only an oral administration of CE with the same dose. Rats from the fourth group, without any treatment, served as control. After 4 weeks of experimentation, AlCl3 exposure showed a significant decrease in sperm concentration (4.58±0.65 × 106 cells /ml), and percentage of viability (61.53±23.63 %), and an increase of morphological abnormalities (26.11±17.84 %). A significant decreased in serum testosterone levels (0.31±0.26 ng/ml) and an increase of testicular malondialdehyde (MDA) level (0.16±0.015 μM/g) were also observed. Histological examination of the testes showed degeneration of the seminiferous tubules, germ line cells, and interstitial cells. However, CE treatment concomitant to AlCl3 showed that the rate of morphological abnormalities (19±2.65 %) is significantly decreased compared to AlCl3 group, with a significant increase in serum testosterone (1.17±0.24 ng/ml) and a significant decrease in MDA (0.11±0.003μM/g) level. Microscopic examination revealed a significant regeneration of seminiferous tubules and interstitial cells. This study demonstrated an ameliorative effect of Curcuma longa aqueous extract in testicular tissue and sperm quality.
Keywords: Curcuma longa, Aluminum, fertility, testosterone, malondialdehyde (MDA), sperm.