bluuebunny/crossref_metadata_2025_split huggingface dataset:
Translation of Evidence Into Practice With Teduglutide in the Management of Adults With Intestinal Failure due to Short‐Bowel Syndrome: A Review of Recent Literature
Authors: Pironi Loris 
Published: December 2019

AbstractChronic intestinal failure (CIF) due to short‐bowel syndrome (SBS) is characterized by failure to achieve optimal intestinal adaptation required to maintain oral/enteral autonomy. The conventional management strategy relies heavily on home parenteral support (PS; parenteral nutrition and/or intravenous fluids). Teduglutide, an analog of the hormone glucagon‐like peptide‐2, facilitates intestinal adaptation, as evidenced by reductions in PS volume in patients with SBS‐associated CIF. In 2016, the European Society for Clinical Nutrition and Metabolism (ESPEN) developed guidelines for the management of adult patients with CIF, consisting of a comprehensive list of recommendations. Owing to the limited number of studies at the time of the finalization of the GRADE‐method review of the available literature, teduglutide received a moderate grade of evidence (GOE) as the first choice for growth‐factor treatment in patients with SBS‐CIF. The GOE was also low for 7 points of recommended information to be discussed with the candidate patients. This review summarizes findings from recent studies that fill some gaps identified in the 2016 ESPEN guidelines regarding the use of teduglutide in the management of SBS‐CIF. Collectively, these studies provide useful information about the probability and timing of clinical response in the individual patient. Also, recent studies report longer‐term safety findings with teduglutide. These results can help physicians better manage patients with SBS‐CIF by aligning clinical decision making with specific disease characteristics, setting the right expectations, and encouraging treatment adherence.
NMR spectroscopy: a powerful tool for the analysis of polyphenols in extra virgin olive oil
Authors: Olmo‐Cunillera Alexandra , López‐Yerena Anallely , Lozano‐Castellón Julián , Tresserra‐Rimbau Anna , Vallverdú‐Queralt Anna , Pérez Maria 
Published: December 2019

AbstractExtra virgin olive oil (EVOO), a key component of the Mediterranean diet, has aroused interest in recent years due to its health properties. Nuclear magnetic resonance (NMR) spectroscopy is an appropriate tool for the accurate quantification of minor compounds in complex food matrices, such as polyphenols in olive oil. Flavonoids, lignans, secoiridoids and phenolic acids and alcohols in EVOO have been identified and quantified by NMR. This review provides an overview of the major developments in the structural elucidation of polyphenol compounds in EVOO. © 2019 Society of Chemical Industry
Stable isotope signatures (δ2H‐, δ13C‐, δ15N‐values) of walnuts (Juglans regia L.) from different regions in Germany
Authors: Krauß Stephanie , Vieweg Annika , Vetter Walter 
Published: December 2019

AbstractBACKGROUNDWalnuts (Juglans regia L.) are considered of high value for human nutrition and are cultivated worldwide. With globalization, however, the demand for regional products has increased. Isotopic fingerprints (δ2H‐, δ15N‐ and δ13C‐values (‰)) created by means of stable isotope ratio mass spectrometry provide the possibility of tracing the geographical provenance of plant foods.RESULTSAuthentic walnut samples from different geographical regions in Germany could be separated from each other by means of their bulk δ2H‐values (‰) with samples from northern regions being more enriched in 2H than samples collected from southern Germany. Next to precipitation data, the topography of the surrounding landscape of the sampling sites was taken into account for data interpretation by the creation of approximate elevation profiles. Moreover, German walnuts could be separated from French walnuts which showed significantly higher δ2H‐values (‰). Variations in annual total precipitation was reflected by the shift in δ2H‐ranges (‰) depending on the harvesting year. Additionally, organic walnuts could be well separated from conventionally produced walnuts due to their distinct δ15N‐values (‰) which could be ascribed to a cultivation practice common for organic farming.CONCLUSIONSThe data presented here outlined the potential of determining isotopic fingerprints of walnuts from different sites within one country for tracing their regional origin and revealing their cultivation conditions. Including topographic characteristics between the sampling locations as well as precipitation data helped to even differentiate walnuts from two adjoining countries. © 2019 The Authors. Journal of the Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
Potential authentication of various meat‐based products using simple and efficient DNA extraction method
Authors: Khairil Mokhtar Nur Fadhilah , El Sheikha Aly Farag , Azmi Nur Izzah , Mustafa Shuhaimi 
Published: December 2019

AbstractBACKGROUNDThe growth of halal food consumption worldwide has resulted in an increase in the request for halal authentication. DNA‐based detection using powerful real‐time polymerase chain reaction (PCR) technique has been shown to be highly specific and sensitive authentication tool. The efficient DNA extraction method in terms of quality and quantity is a backbone step to obtain successful real‐time PCR assays. In this study, different DNA extraction methods using three lysis buffers were evaluated and developed to recommend a much more efficient method as well as achieve a successful detection using real‐time PCR.RESULTSThe lysis buffer 2 (LB2) has been shown to be the best lysis buffer for DNA extraction from both raw and processed meat samples comparing to other lysis buffers tested. Hence, the LB2 has been found to be ideal to detect meat and porcine DNAs by real‐time PCR using pairs of porcine specific primers and universal primers which amplified at 119 bp fragment and 93 bp fragment, respectively. This assay allows detection as low as 0.0001 ng of DNA. Higher efficiency and sensitivity of real‐time PCR via a simplified DNA extraction method using LB2 have been observed, as well as a reproducible and high correlation coefficient (R2 = 0.9979) based on the regression analysis of the standard curve have been obtained.CONCLUSIONThis study has established a fast, simple, inexpensive and efficient DNA extraction method that is feasible for raw and processed meat products. This extraction technique allows an accurate DNA detection by real‐time PCR and can also be implemented to assist the halal authentication of various meat‐based products available in the market. © 2019 Society of Chemical Industry
3D Network Binder via In Situ Cross‐Linking on Silicon Anodes with Improved Stability for Lithium‐Ion Batteries
Authors: Li Pengcheng , Chen Gen , Lin Yifan , Chen Fashen , Chen Long , Zhang Ning , Cao Yijun , Ma Renzhi , Liu Xiaohe 
Published: December 2019

AbstractSilicon (Si) has attracted intensive academic and commercial attention due to its extremely high theoretical capacity. However, it is still far away from practical application because of its fast capacity fading, which is caused by the huge volume change. Here, a novel network polymer binder is synthesized through in situ thermal crosslinking of water‐soluble carboxymethyl cellulose (CMC) and maleic anhydride (MAH). The as‐obtained polymer binder network can effectively restrict the huge volume change of Si anodes upon lithiation. Because of the significantly enhanced structural stability, the Si anodes with network polymer binder deliver enhanced electrochemical performance, with a capacity of 996 mAh g−1 at a high current density of 1 A g−1 after 120 cycles under high mass loading. Most importantly, a high average Coulomb efficiency (CE) of 99.4% is obtained, which is superior over the average CE (98.7%) of Si only using CMC as binder. It is considered that this novel 3D network cross‐linking binder can be used for high‐capacity anode materials in next‐generation Li‐ion batteries.
An inline waveguide‐to‐microstrip transition for wideband millimeter‐wave applications
Authors: Li Cun Long , Jin Cheng , Ma Han Qing , Shi Xiao Wei 
Published: December 2019

AbstractA low‐cost wide Ka‐band inline waveguide‐to‐microstrip transition is proposed, which facilities the integration of planar transmission lines with waveguide structures. By employing a circular patch above a wedge‐shaped cavity, the quasi‐TEM mode wave in the microstrip line can be transformed into the TE10 mode wave in the rectangular waveguide. A transition prototype in a back‐to‐back configuration is manufactured and experimentally tested. Measured results show that the return loss is greater than 13 dB from 28 to 38 GHz and the insertion loss remains less than 0.4 dB for the proposed single‐side transition. With the features of wideband, compact structure and easy integration, the proposed transition can be applied in the low‐loss and high‐integration millimeter‐wave systems.
Metabolomic profiling in neuroblastoma
Authors: Quintás Guillermo , Yáñez Yania , Gargallo Pablo , Juan Ribelles Antonio , Cañete Adela , Castel Victoria , Segura Vanessa 
Published: December 2019

AbstractBackground and objectivesPrevious studies on several cancer types show that metabolomics provides a potentially useful noninvasive screening approach for outcome prediction and accurate response to treatment assessment. Neuroblastoma (NB) accounts for at least 15% of cancer‐related deaths in children. Although current risk‐based treatment approaches in NB have resulted in improved outcome, survival for high‐risk patients remains poor. This study aims to evaluate the use of metabolomics for improving patients’ risk‐group stratification and outcome prediction in NB.Design and methodsPlasma samples from 110 patients with NB were collected at diagnosis prior to starting therapy and at the end of treatment if available. Metabolomic analysis of samples was carried out by ultra‐performance liquid chromatography‐time of flight mass spectrometry (UPLC‐MS).ResultsThe metabolomic analysis was able to identify different plasma metabolic profiles in high‐risk and low‐risk NB patients at diagnosis. The metabolic model correctly classified 16 high‐risk and 15 low‐risk samples in an external validation set providing 84.2% sensitivity (60.4‐96.6, 95% CI) and 93.7% specificity (69.8‐99.8, 95% CI). Metabolomic profiling could also discriminate high‐risk patients with active disease from those in remission. Notably, a plasma metabolomic signature at diagnosis identified a subset of high‐risk NB patients who progressed during treatment.ConclusionsTo the best of our knowledge, this is the largest NB study investigating the prognostic power of plasma metabolomics. Our results support the potential of metabolomic profiling for improving NB risk‐group stratification and outcome prediction. Additional validating studies with a large cohort are needed.
Capture of biologic and biosimilar dispensings in a consortium of U.S.‐based claims databases: Utilization of national drug codes and Healthcare Common Procedure Coding System modifiers in medical claims
Authors: Zhang Jie , Haynes Kevin , Mendelsohn Aaron B. , Marshall James , Barr Charles E. , McDermott Cara , Brown Jeffrey , Kline Annemarie , Kenney James , King Katelyn J. , Holmes Cynthia , Yeung Kai , Barron John , Yun Huifeng , Lockhart Catherine M. 
Published: December 2019

AbstractPurposeTo assess the capture of biologics (originator and biosimilar) in the Biologics and Biosimilars Collective Intelligence Consortium (BBCIC) Distributed Research Network (DRN), with a focus on medical claim National Drug Code (NDC), a new data field, and Healthcare Common Procedure Coding System (HCPCS) modifier.MethodsWe conducted a repeated cross‐sectional study among patients with medical and pharmacy benefits enrolled in insurance plans participating in the BBCIC DRN between 1 January 2013 and 30 September 2017. We calculated the proportion of medical claims with ≥1 NDC and identified select biologics using four different approaches: (a) specific HCPCS alone, (b) specific HCPCS and NDC, (c) non‐specific HCPCS with NDC, and (d) HCPCS with modifiers (applicable to biosimilars). Numbers of dispensings were calculated for each biologic by approach and select patient and claim characteristics.ResultsMore than 1.5 million eligible participants contributed approximately 4 million person‐years of data, including 1.2 billion medical claims. The proportion of medical claims with ≥1 NDC increased from 1.2% in 2013 to 3.0% in 2017. Medical claim NDCs identified 39% and 28% of vedolizumab dispensed in 2014 and 2015 and 30% of Epogen/Procrit dispensed overall. Out of 26,381 filgrastim biosimilar dispensings identified, 51% had a HCPCS modifier and 12% had a medical claim NDC for Zarxio. HCPCS modifiers and medical claim NDCs were present for 38% and 3% of all infliximab biosimilars dispensed (total n = 1,244).ConclusionsMedical claim NDC and HCPCS modifier improves identification of select biologics without product‐specific HCPCS code, thereby facilitating product‐specific biologic research.
Nanosecond laser scribing for see‐through CIGS thin film solar cells
Authors: Kuk Seungkuk , Wang Zhen , Yu Hyeonggeun , Nam Chang‐Yong , Jeong Jeung‐hyun , Hwang David 
Published: December 2019

AbstractBuilding‐integrated photovoltaic (BIPV), especially in a semitransparent and/or see‐through configuration, has attracted significant attention because of the extended surfaces available for the photovoltaic (PV) installation including roofs, facades, and windows. In this study, we examine the P4 scribing process for fabricating see‐through cells on a new Cu (In,Ga)Se2 (CIGS) architecture with indium tin oxide (ITO) bottom contact, using a nanosecond laser beam of 532‐nm wavelength illuminated from glass substrate side. Through parametric studies with the variations of laser beam spot size and pulse energy, we have identified that enlarged laser beam with the pulse energy near scribing threshold could suppress both damage in ITO and electrical shunt induced by molten CIGS. Scanning electron microscopy (SEM) and energy‐dispersive X‐ray spectroscopy (EDS) analyses unveil that the molten CIGS mediated shunt mechanism, wetting the scribing edge and forming Cu‐rich metallic phase. The P4 scribing process operated near threshold fluence of enlarged laser beam clearly suppressed unwanted shunt, also minimizing the fluctuation in the desired film removal trend. Thermal analysis supports that enlarged laser beam enables scribing at reduced CIGS‐ITO interface temperature assisted by buckling‐based film delamination mechanism and also suppresses CIGS melting at scribing edge and its neighborhood. See‐through cells fabricated for the areal fraction of approximately 15% using the optimal laser scribing parameters exhibited the short circuit current reduction rate of 16.8% enabled by the low shunt resistance reduction rate of approximately 8%. Further studies are underway to elucidate precise shunt‐related scribing mechanism on the basis of the cross‐sectional analyses and time‐resolved diagnostics and to fabricate the module level see‐through PV architectures.
Arabidopsis cytosolic acyl‐CoA‐binding proteins function in determining seed oil composition
Authors: Guo Ze‐Hua , Ye Zi‐Wei , Haslam Richard P. , Michaelson Louise V. , Napier Johnathan A. , Chye Mee‐Len 
Published: December 2019

AbstractAs plant seed oils provide animals with essential fatty acids (FAs), genes that regulate plant lipid metabolism have been used in genetic manipulation to improve dietary seed oil composition and benefit human health. Herein, the Arabidopsis thaliana cytosolic acyl‐CoA‐binding proteins (AtACBPs), AtACBP4, AtACBP5, and AtACBP6 were shown to play a role in determining seed oil content by analysis of atacbp (atacbp4, atacbp5, atacbp6, atacbp4atacbp5, atacbp4atacbp6, atacbp5atacbp6, and atacbp4atacbp5atacbp6) seed oil content in comparison with the Col‐0 wild type (WT). Triacylglycerol (TAG) composition in electrospray ionization‐mass spectrometer (ESI‐MS) analysis on atacbp6 seed oil showed a reduction (−50%) of C58‐TAGs in comparison with the WT. Investigations on fatty acid composition of atacbp mutants indicated that 18:2‐FA accumulated in atacbp6 and 18:3‐FA in atacbp4, both at the expense of 20:1‐FA. As TAG composition can be modified by acyl editing through phosphatidylcholines (PC) and lysophosphatidylcholines (LPC), total PC and LPC content in atacbp6 mature seeds was determined and ESI‐MS analysis revealed that LPC had increased (+300%) at the expense of PC. Among all the 14 tested PC species, all (34:1‐, 34:2‐, 34:3‐, 34:4‐, 34:5‐, 34:6‐, 36:2‐, 36:3‐, 36:5‐, 36:6‐, 38:2‐, 38:3‐, and 38:4‐PCs) but 36:4‐PC were lower in atacbp6 than the WT. In contrast, all LPC species (16:0‐, 18:1‐, 18:2‐, 18:3‐, and 20:1‐LPC) examined were elevated in atacbp6. LPC abundance also increased in atacbp4atacbp5, but not atacbp4 and atacbp5. Interestingly, when LPC composition in atacbp4atacbp5 was compared with atacbp4 and atacbp5, significant differences were observed between atacbp4atacbp5 and each single mutant, implying that AtACBP4 and AtACBP5 play combinatory roles by affecting LPC (but not PC) biosynthesis. Furthermore, PC‐related genes such as those encoding acyl‐CoA:lysophphosphatidylcholine acyltransferase (LPCAT1) and phospholipase A2 alpha (PLA2α) were upregulated in atacbp6 developing seeds. A model on the role of AtACBP6 in modulating TAG through regulating LPCAT1 and PLA2α expression is proposed. Taken together, cytosolic AtACBPs appear to affect unsaturated TAG content and are good candidates for engineering oil crops to enhance seed oil composition.
GenFam: A web application and database for gene family‐based classification and functional enrichment analysis
Authors: Bedre Renesh , Mandadi Kranthi 
Published: December 2019

AbstractGenome‐scale studies using high‐throughput sequencing (HTS) technologies generate substantial lists of differentially expressed genes under different experimental conditions. These gene lists need to be further mined to narrow down biologically relevant genes and associated functions in order to guide downstream functional genetic analyses. A popular approach is to determine statistically overrepresented genes in a user‐defined list through enrichment analysis tools, which rely on functional annotations of genes based on Gene Ontology (GO) terms. Here, we propose a new computational approach, GenFam, which allows annotation, classification, and enrichment of genes based on their gene family, thus simplifying identification of candidate gene families and associated genes that may be relevant to the query. GenFam and its integrated database comprises of three hundred and eighty‐four unique gene families and supports gene family analyses for sixty plant genomes. Four comparative case studies with plant species belonging to different clades and families were performed using GenFam which demonstrated its robustness and comprehensiveness over preexisting functional enrichment tools. To make it readily accessible for plant biologists, GenFam is available as a web‐based application where users can input gene IDs and export enrichment results in both tabular and graphical formats. Users can also customize analysis parameters by choosing from the various statistical enrichment tests and multiple testing correction methods. Additionally, the web‐based application, source code, and database are freely available to use and download. Website:https://www.mandadilab.com/genfam/. Source code and database:https://www.mandadilab.com/dload/.
Plasma deposition of collagen for cell‐culture applications
Authors: O'sullivan Denis , McArdle Hazel , O'Reilly Julie‐Ann , O'Kennedy Richard J. , Forster Robert , O'Neill Liam 
Published: December 2019

AbstractCollagen coating of polystyrene (PS) labware involves a multistep process with high costs and complexity. Nonthermal plasma processes have recently been found to deposit coagulated and biologically functional protein layers in a single process step. A series of investigations were, therefore, conducted to compare plasma‐deposited collagen to an equivalent commercially coated product. Chemical and microscopic analysis suggests that the plasma‐deposited collagen retains the surface morphology and functional chemistry of the traditional collagen coating. Cell‐culture studies using two separate cell lines confirmed that the plasma‐deposited materials replicated the cell viability, proliferation and activity of the traditional collagen surfaces and outperformed uncoated PS plates. The implications of this for the protein coating of plastic labware surfaces are discussed.
A hybrid human reliability assessment technique for the maintenance operations of marine and offshore systems
Authors: Islam Rabiul , Anantharaman Mohan , Khan Faisal , Abbassi Rouzbeh , Garaniya Vikram 
Published: December 2019

AbstractRegular maintenance is very important to ensure all the required types of machinery and equipment be kept 100% efficient for marine and offshore systems. Maintenance operations for marine and offshore systems are carried out by the seafarers/operators and it is they who are usually liable for any error. Previous studies have identified that about 80% of marine and offshore accidents occurred due to human error. Therefore, to address this concern human reliability assessment (HRA) is very important. However, an appropriate technique is required to estimate human error probability (HEP) for marine and offshore systems. Human error assessment and reduction technique (HEART) is applied to many industries to determine HEP. Recently, HEART was specifically developed for the maintenance operations of marine and offshore systems considering marine and offshore environmental and operational conditions. However, there is a deficiency in this technique as it does not provide a concrete method to determine the seafarers assessed proportion of effect (SAPOE) and it therefore, relies heavily on the judgment of a single expert. This study proposes a hybrid HEART to overcome the problem. The hybrid HEART utilizes the evidence theory to fuse an expert's opinion to determine APOA for each corresponding error producing condition (EPC). The proposed technique is applied to estimate HEP for the maintenance procedures of a condensate pump for an offshore oil and gas facility as a case study. The HEP values are calculated for each selected activity and comparison is provided. Based on the results, a performing pressure test and isolation leak test has the highest HEP 1.54E‐01 and depressurizing drain lines has the lowest 1.54E‐04. It is proposed that the application of this hybrid HEART will enable estimating HEP more accurately. Therefore, it will contribute to improving the overall safety level in the maintenance of marine and offshore systems.