bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Agonist of JWA Gene JAC1 Suppresses Proliferation of Breast Cancer Through the JWA/p38/SMURF1/HER2 Signaling
Authors: Ren Yanlin , Chen Dongyin , Chen Junjie , Zhai Zurong , Li Aiping , Liang Yan , Zhou Jianwei 
Published: June 2020

Abstract
Background

The overexpression of HER2 is associated with malignant proliferation and invasiveness in breast cancer. Although HER2-targeting drugs have been clinically applied for cancer treatment, none of them could reduce overexpressed HER2. In this study, we reported that JAC1 could suppress proliferation of breast cancer cells via degrading HER2.
Methods

JWA-HER2 association was analyzed by IHC in 90 paired cases of breast cancer and adjacent non-cancerous tissues. Regulatory effect of JAC1, the agonist of JWA gene, on HER2-positive breast cancer cells was studied using colony formation assay. The effect of JAC1 on the localization of HER2 was detected by immunofluorescence microscopy assay. Western blotting, RT-PCR and immunoprecipitation assay were utilized to investigate the mechanisms of JWA on regulating HER2. Finally, xenograft mouse models were established in nude mice using BT474 cells to confirm the effect of JAC1 in vivo.
Results

JAC1, a small molecule agonist of JWA gene, dose-dependently suppressed proliferation in HER2-positive breast cancer in vitro and in vivo through degrading HER2. The mechanistic evidences showed that JAC1 increased the ubiquitination of HER2 at the K716 through the E3 ubiquitin ligase SMURF1. Furthermore, SMURF1 was activated due to reduced expression of NEDD4, an E3 ubiquitin ligase for SMURF1 through the JWA-p38-GATA-1-NEDD4 axis.
Conclusions

JAC1 suppresses the proliferation in HER2-positive breast cancer through the JWA/p38/GATA-1/NEDD4/SMURF1/HER2 signaling. JAC1 may serve as a novel therapeutic agent to breast cancer.
Inhibition of Carnitine Palmitoyl Transferase 1A-induced Fatty Acid Oxidation Suppresses Cell Progression in Gastric Cancer
Authors: Wang Liqiang , Li Changfeng , Song Yumei , Yan ZhenKun 
Published: June 2020

Abstract
Background: Gastric cancer (GC) has a high rate of metastasis which thereason leading to death. Carnitine palmitoyltransferase 1a (CPT1A) has been reported to play a critical obstacle to various types of cancer progression, which is an attractive focus in anti-cancer therapy. However, the underlying molecular mechanisms of CPT1A involved in GC have not been clarified unclear.Methods: To determine the expression of CPT1A in human GC tissues and cells and illustrate whether it is correlated with the clinical pathologic characteristics and prognosis in GC patients. Its roles and potential mechanisms in regulating tumor growth and invasion were evaluated by CPT1A knockdown/overexpression of GC cells in vitro.Results: Marked upregulation of CPT1A protein expression was observed in GC cells and tissues, which was associated with grade, pathological stage, lymph node metastasis and poor prognosis in patients with GC. CPT1A overexpression also promoted the proliferation, invasion, EMT process of GC cells. In addition, CPT1A upregulation activated GC cell FAO via increasing NADP+/NADPH ratio, whereasinhibiting of FAO abolished the effects of CPT1A on GC cell proliferation and migration.Conclusion: Our results examine that CPT1A-mediated FAO activation increases GC cell proliferation and migration, supporting that CPT1A is a useful prognostic biomarker and an attractive focus for GC.
Evaluation of in vitro and in vivo Antiplasmodial Activity of the Leaf Latex of Aloe Weloensis (Aloaceae)
Authors: Amare Gedefaw Getnet , Awgichew Tadesse , Ahmed Solomon , Kifle Zemene Demelash 
Published: June 2020

Abstract
Background: Nature has gifted a variety of plants having potential effect against plasmodium parasites. The present study was aimed to determine in vitro and in vivo antimalarial activity of the leaf latex of Aloe weloensis.Methods: In vitro antimalarial activity of the leaf latex of A. weloensis was determined against 3D7 strain of P. falciparum. Antimalarial activity of the three doses the latex was evaluated in 4 day-suppressive and curative models against P. berghei infected mice. Antioxidant activity of the leaf latex of A. weloensis was assessed in 2,2- diphenyl 1- picrylhydrazine assay model. Results: Antioxidant activity of the latex was concentration dependent; the strongest inhibition was measured at 400 μg/mL (73.54%). The leaf latex of A. weloensis was demonstrated inhibitory activity against 3D7 malarial strain (IC50 = 9.14 μg/ml). Suppressive and curative effect of the latex was found to be dose dependent. Parasitemia reduction was significant (200 mg/kg, p<0.01, 400 and ,600 mg/kg, p<0.001) in 4-day suppressive test compared to vehicle control. Parasitemia level of the mice treated with 200, 400 and 600 mg/kg doses of the latex significantly (p<0.001) reduced with suppression of 36%, 58% and 64% respectively in curative test. Administration of the leaf latex of A. weloensis significantly (p<0.01) improved mean survival time, pack cell volume, rectal temperature and body weight of P. berghei infected mice. Conclusion: The finding showed that the leaf latex of Aloe weloensis endowed prominent antimalarial and antioxidant activities. The result can serve as a step towards the development of safe and effective herbal therapy against plasmodium parasites.
Risk Prediction and Treatment of Hemorrhagic Chronic Radiation Intestinal Injury Patients With LE-DVT
Authors: Huang Xiaoyan , Kuang Yingyi , Yuan Zixu , Zhu Miaomiao , He Yanjiong , Wang Huaiming , Zhong Qinghua , Guan Qi , Wang Hui , Ma Tenghui 
Published: June 2020

Abstract
Background: After pelvic malignancy radiation, chronic radiation intestinal injury (CRII) is an unavoidable complication, and bleeding is one of the most common symptoms of CRII. Lower extremity deep venous thrombosis (LE-DVT) is another severe complication.Once hemorrhagic CRII patients suffer from LE-DVT, hemostasis and anticoagulation therapy will be adopted simultaneously, which is a therapeutic paradox, extremely intractable and serious. This study was aimed to investigate prevalence and risk factors for LE-DVT in CRII patients, and explore treatment for hemorrhagic CRII patients with LE-DVTMethods: This is a retrospective study,a total of 608 CRII hospitalized patients from November 2011 to October 2018 after pelvic malignancy radiation in our hospital were included.Univariate and multivariate analysis were conducted to investigate the associated risk factors for LE-DVT in CRII patients. Receiver operating characteristic (ROC) curve analysis was performed to investigate the independent risk factors and determine their clinically valid cut-off points. Furthermore, treatment of hemorrhagic CRII patients with LE-DVT was explored.Results: Of the 608 included CRE patients, there were 94 (15.5%) CRII patients with suspicious symptoms of LE-DVT in the lower limbs, and 32 (5.3%) patients were diagnosed with LE-DVT. Among the CRII patients with LE-DVT, 65.6% (21/32) patients were with bleeding simultaneously, 29 (90.6%) patients were anemic, with 17 (53.1%) patients having moderate anemia and 7 (21.9%) having severe anemia. Multivariate analysis showed recent surgical history (≤6 Months) (OR=0.480, 95% CI: 1.430~9.377, p=0.007) and hemoglobin (Hb) (OR=0.965, 95% CI: 0.945~0.986, p=0.001) significantly associated with development of LE-DVT. ROC curve analysis showed optimal cut-off values of Hb were 82.5 g/L (AUC=0.756, 95% CI: 0.688~0.824, sensitivity=71.9%, specificity=75.5%). After colostomy, obvious bleeding remission was found in 84.6% of hemorrhagic CRII patients with LE-DVT rapidly. And LE-DVT of the patients was obvious improved or disappeared following anticoagulation therapy or with vena cava filter or stent placement.Conclusions: Prevalence of LE-DVT in hospitalized CRII patients was 5.3%. Recent surgical history and lower Hb were independently associated with LE-DVT developing in CRII patients. And colostomy could be a good choice for intractable hemorrhagic CRII patients with LE-DVT.
Synthesis of 7, 8 –dihydroxyflavone functionalized gold nanoparticles and its mechanism of action against Leishmania donovani
Authors: Prasanna Pragya , Kumar Prakash , Mandal Saptarshi , Payal Tanvi , Kumar Saurabh , Das Prolay , Ravichandiran V , mandal debabrata 
Published: June 2020

Abstract
Abstract
Background – The synthesis of gold nanoparticles (GNPs) using drugs, synthetic and natural compounds, proteins, nucleic acids have become beneficial due to improved biological activity coupled with reduced cytotoxicity. In this regard, green synthesis of GNPs using plant extract enriched with flavonoids has shown increased attention due to improved antimicrobial efficacy, greater solubility, and better bioavailability of the flavonoid conjugated with GNPs. We have used 7, 8 dihydroxyflavone (DHF), a flavonoid that is enriched in plants and known for neurotropic and antioxidant activities, for the synthesis of GNP. In this report, we have investigated synthesis, characterization, and biological activity of DHF synthesized GNP against parasite Leishmania donovani .
Results – Synthesized DHF functionalized GNPs (DHF-GNPs) are ~35 nm in size with zeta potential values of -34.1 mV, as observed from DLS studies. UV-Visible spectroscopy and FT-IR analysis confirms successful conjugation of DHF over GNP. TEM imaging shows uniform size and spherical distribution of NPs. Against L. donovani promastigotes IC 50 for DHF and DHF-GNP is ~140 µM and ~40 µM respectively. In ex vivo amastigote model, IC 50 for DHF and DHF-GNP is ~40 µM and ~22 µM respectively. Even with 1000 µM of DHF-GNP, cytotoxicity is only ~30% on THP1 cells indicating its high biocompatibility. In DHF-GNP treated parasites, activity of arginase decreases in a dose-dependent manner as evident from gene expression and enzyme-based studies. Supplementation of treated cells with ornithine, metabolite of arginase, shows the highest recovery from death. This indicates inhibition of arginase as the main reason for parasite death. Induction of IFN-γ and reduction IL-12 cytokine response shows a possible T h 1/T h 2-mediated cell death. Also, DHF and DHF-GNP are equally effective against sensitive and drug-resistant strains of L. donovani .
Conclusion – Low cytotoxicity and high biological activity may provide an alternative but improved delivery of DHF whose solubility increases due to conjugation with GNP. Further efficacy against drug-resistant strains could be beneficial instead of conventional chemotherapy for leishmaniasis.

Keywords : Functionalized gold nanoparticle, DHF, DHF-GNP, arginase inhibition, drug-resistant
Parasite, iNOS activation
Days away from Work Injury and Associated Factors among Waste Collectors in Mekelle City, Northern Ethiopia: A Cross Sectional Study
Authors: Abrha Mesele Bahre , Messelle Yisak Arbise , Asgedom Akeza Awealom , Meressa Beyene 
Published: June 2020

Abstract
Background: In its nature, solid waste management is a labour intensive industry, which exposes workers to numerous occupational hazards. This study was aimed to investigate the magnitude of days away from work injuries and associated factors among organized waste collectors in Mekelle city, Northern Ethiopia. Method: A questionnaire and observation checklist based cross-sectional study design was employed from June 1 to 30, 2017. Data was analysed using SPSS for windows 20.0. Descriptive statistics and logistic regression methods were used to describe the study population and assess the association between dependent and independent variables, respectively. Result: From the total of 279 waste collectors involved as a study participant, ten percent(10%) of them reported at least one day away from work injuries during the last twelve months. Female were 96% less likely to be injured as compared to male (AOR = 0.04, 95%CI: 0.008 -0.204). Being married is 87% less likely to be injured as compared to being a single (AOR = 0.130 95% CI: 0.027-0.621). The odds of injury were 4.5 times higher among those who do not use personal protective equipment as compared to their counterparts (AOR = 4.514 95%CI: 1.684-12.095). Waste collectors, who had less than 1000 Birr income, were 3 times more likely to be injured than waste collectors who had greater than 1001 Birr per month income (AOR = 3.008 95%CI: 1.081 - 8.371.Conclusion: Days away from work injury among waste collectors is a public health problem and has an impact on the economic and social well-being of workers. Therefore, strengthening the provision of personal protective devices and insuring its utilization is highly recommended.
Metabolic Healthy Obesity is associated with higher incidence of mild decrease estimate glomerular rate in rural Northeast Chinese
Authors: Yu Shasha , Guo Xiaofan , Li GuangXiao , Yang Hongmei , Zheng Liqiang , Sun Yingxian 
Published: June 2020

Abstract
Background
Metabolic healthy obesity (MHO), as one phenotype of obesity, seems associate with lower risk of cardiovascular disease. However, MHO has close relationship with higher incidence of metabolic syndrome and diabetes. This study aims to investigate the prevalence of MHO at baseline, changes of obese metabolic phenotype at follow-up and its relationship with incidence of mildly reduced estimated glomerular filtration rate (eGFR) in rural Northeast Chinese.
Methods
The Chronic Kidney Disease Epidemiology (CKD-EPI) equation was used to calculate eGFR. 4903 participants aged ≥ 35 years with eGFR > 90 ml/min/1.73 m2 at baseline were enrolled and successfully followed up. All participants completed the questionnaires, anthropometric measurements, and blood test during baseline and follow-up. Mild renal dysfunction defined as mildly reduced eGFR between 60–90 ml/min/1.73 m2.
Results
The prevalence of MHO was 20.04% in baseline (18.97% for women and 21.11% for men) which was secondary to metabolic abnormal obesity (MAO) (24.4%, 27.2% for women and 21.5% for men). 38.4% of women and 38.90% of men experienced phenotype changes during follow-up. The cumulative incidence of mildly reduced eGFR in MHO was 20.1% (17.7% for women and 22.3% for men) which was also secondary to MAO (20.8%, 18.6% for women and 23.5% for men). After adjusted possible confounders, MHO was associated with higher incidence of mildly reduced eGFR among women [OR (95%CI) = 1.64 (1.18, 2.25)] and men [OR (95%CI) = 1.62 (1.24, 2.11)] whereas MAO was related with higher incidence of mildly reduced eGFR among men only [OR (95%CI) = 1.74 (1.32, 2.29)].
Conclusion
MHO was associated with higher incidence of mildly reduced eGFR in both gender; however, there was a specific relationship between MAO and mildly reduced eGFR in men only. Therefore, it is necessary to monitoring kidney function among both MHO and MAO subjects.
Healthy Lifestyle Behaviors Are Major Predictors of Mental Wellbeing During COVID-19 Pandemic Confinement: A Study on Adult Arabs in Higher Educational Institutions
Authors: Kilani hashem A. , Bataineh  , Al-Nawayseh Ali , Atyat Khaled , Obeid Omar , Abu-Hilal Maher M. , Mansi Taiysir , AlKilani Maher , Al-Kitani Mahfooda , Jaber Ruba M. , Sweidan Ahmad , Himsi Mawaheb , Yousef Iyad , Alzeer Faten , Nasrallah Monther , Dhaheri Ayesha S Al , Al-Za’abi Abdulsalam , Allala Osama , Al-Kilani Laila , El-Saleh Majed , Alhasan Asma M , Ghieda Mohamed , Najah Yasir , Alsheekhly Saad , Alhaifi Ahmad , Shukri Raghda , Adwani Jamal Al , Waly Mostafa , Kilani Laila H. , Kilani Leen H , Shareef Ahmad S Al , Kilani Areej H. 
Published: June 2020

Abstract
Background
In previous times, infectious diseases affected the quality of human life during home confinement. This study investigated the influence of home confinement during the COVID-19 pandemic outbreak on lifestyle, mental wellbeing, nutritional status, and sleeping pattern.
Method:
An online multicategory questionnaire was distributed to collect Demographical information and combined the following tools: Food Frequency Questionnaire (FFQ), International Physical Activity Questionnaire (IPAQ), WHO-5 wellbeing score, and Pittsburgh Sleep Quality Index (PSQI). A snowball none-discriminate sampling procedure was followed to collect data from people attending or working at higher institution had covering the period between Mar/00/2020 and Apr/24/2020. A total of 1723 completed responses (917 Males 37.4 ± 13.4 yrs. old and (806 Females 32.2 ± 11.5 yrs. old) were collected.
Results
Female participants had significantly lower mental health scores as compared to males (53.9% vs 46.1%). on Mental wellbeing score was better among participants with medium and high Physical Activity Levels (p < 0.00). Additionally, mental wellbeing score was significantly improved by dietary quality and sleeping score (p < 0.001). However, physical activity was by far the major determinants of mental health score.
Conclusion
Factors such as PA, Diet, sleeping patterns were associated with mental wellbeing during COVID-19 confinement among Arab participants.
Accuracy of the chest x-ray in screening for tuberculosis in Uganda: A cross-sectional study.
Authors: Nalunjogi Joanitah , Mugabe Frank , Najjingo Irene , Lusiba Pastan , Olweny Francis , Mubiru Joseph , Kayongo Edward , Sekibira Rogers , Katamba Achilles , Kirenga Bruce 
Published: June 2020

Abstract
ABSTRACT

BACKGROUND: The WHO END TB strategy requires ≥90% case detection to combat tuberculosis (TB). Increased TB case detection requires a more sensitive and specific screening tool. Currently, the symptoms recommended for screening TB have been found to be sub-optimal. CXR as a screening tool for pulmonary TB was evaluated in this study, as well as factors related to its false positive results.

METHODS: A cross sectional study of 4441 records of consented/assented participants ≥15 years. Participants with a cough ≥2 weeks and/or any abnormality in the lung on CXR were included in the study. Löwenstein-Jensen (LJ) culture was used as the gold standard. The CXR were categorised as Abnormal meaning presence of any CXR abnormality suggestive of active tuberculosis. Symptoms were categorised as abnormal meaning presence of any of cough ≥ 2 weeks, fever, weightloss or night sweats.

RESULTS: The CXR had sensitivity 93%, specificity 65% compared to culture results while symptoms had sensitivity 76% and specificity 31%. The adjusted prevalence ratio (APR) of a false positive CXR result increased with age categories (years); 45 - 54, APR 1.18 (1.08, 1.29), 55 – 64 APR 1.18 (1.09, 1.29), 65+years APR 1.2 (1.10, 1.30).The APR was 0.93 (0.90, 0.96) among males and 0.86 (0.79, 0.93) among HIV positive individuals.

CONCLUSIONS: The CXR is a fair tuberculosis screening tool and performed better than symptoms in Uganda.
The Impact of COVID-19 on the Chiropractic Profession: A Cross-Sectional Survey on opinions, professional changes, and personal hardships of U.S. Chiropractors
Authors: Neff Shawn M , Deyo Rebecca L. , Auley Annabelle L. Mac , Lawrence Dana J. 
Published: June 2020

Abstract
Background : Currently, there are few studies analyzing how COVID-19 has impacted the chiropractic profession. A recent paper, however, analyzed changes made to chiropractic practice by comparing the 7 different international regions represented by the World Federation of Chiropractic (WFC). In overall healthcare, there is limited research on specialty-based stress levels both before and during COVID-19. Our survey was conducted to collect self-reported data on how COVID-19 has affected U.S. doctors of chiropractic.
Methods : An electronic survey was sent to U.S. chiropractors nationwide via social media, email, and through Facebook groups. The survey collected personal and practice demographic information, practice protocols and changes made during COVID-19, chiropractic profession opinions/stances, information related to stress, and personal beliefs/opinions. Data was analyzed using descriptive statistics.
Results : 750 U.S. chiropractors responded. Just over half of respondents reported moderate levels of stress, and just over 30% reported severe levels due to a variety of reasons related to both personal and professional circumstances. The primary stressors were financial and business concerns. The highest stress levels were among employers responsible for others. A majority reported beliefs that the chiropractic profession should not advertise that spinal manipulation provides any immune system benefit. 13% of the respondents did believe chiropractors should be marketing immune benefits during this time. A shift toward telehealth was reported, with 18% adding it to their services.
Conclusion : Stress levels were high across the population. A range of opinions existed regarding spinal manipulation and immunity benefits. The majority reported there was not sufficient evidence to support such a belief; however, 13% of respondents believe that chiropractors should be marketing immune-enhancing benefits to the population. A logical next step would be to study why such beliefs persist. This information may be useful in better understanding how chiropractors have experienced the global effects of COVID-19 across the United States.
Key Words : Chiropractic; COVID-19; Stress; Practice Characteristics; Immune System
Antimicrobial resistance and epidemiology of ESBLs-producing Escherichia coli and Enterobacter cloacae isolates from the intensive care unit in an affiliated hospital of University, China
Authors: Chen Kun , Yang Guoliang , Li Wenping , Li Mingcheng 
Published: June 2020

Abstract
Background：Concerns are increasing over the importance of the hospital intensive care units (ICU) for the transmission of extended spectrum-β-lactamase (ESBLs) -producing Enterobacteriaceae. We reported the clinical characteristics and epidemiology of ESBLs isolates collected from a tertiary care hospital in China. Methods：Escherichia coli（E. coli）and Enterobacter cloacae （E. cloacae）isolates from ICU infection samples were isolated and identified. Antimicrobial susceptibility profiles and production of ESBLs were determined by using the disk diffusion method and the broth microdilution method. Clonality of isolates was determined by ERIC-PCR techniques. Results：From the included the 223 strains isolated from hospitalized patients with nosocomial infections in ICU during 2016 to 2018, the majority of isolates belonged to Gram-negative Enerobacteriaceae including E. coli (46.6% of all strains), and E. cloacae (46.2% of all strains). 63.25% of samples were separated from sputum or tracheal secretions. All of 207 isolates, ESBL-screen positive E. coli was 45.2% (47/104), and 44.7% (46/103) for E. cloacae. Resistance rates of ESBLs-producing E. coli and E. cloacae isolates were 95.5%-91.3% for ampicillin, 80.6%-76.1% for ampicillin/azobactam, 88.1%-28.3% for ciprofloxacin, 89.6%-15.2% for levofloxacin, 34.3%-45.7% for netilmicin, 82.1%-41.3% for compound sulfamethoxazole, 20.9%-43.5% for amikacin, 58.2%-37.0% for gentamicin, 20.9%-69.6% for piperacillin/tazobactam. All of ESBLs-producer isolates resistant to cefazolin, cefuroxime, ceftazidime, ceftriaxone, cefepime in additon to aztreonam were 100%, whereas the susceptibilities of isolates to imipenem and meropenem were 100%. Results of ERIC-PCR in all of ESBLs-producing E. coli isolates exhibited 11 distinct patterns using a similarity coefficient of 0.8. And one distinct ERIC profiles were observed amongst 46 strains of ESBLs-producing E. cloacae. ERIC profiles demonstrated an outbreak of nosocomial infection and ESBLs-producing E. coli and E. cloacae prevalent in the ICU of this hospital.Conclusions：Our data indicate that the ESBLs-producing E. coli and E. cloacae clones are circulating in the ICU and constitute a major source for further disseminating in this hospital. It is necessary to increase surveillance and development of adequate prevention strategies.