bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of Cladosporium sp. Fungi by in- silico RFLP-PCR
Authors: Khalil Mohammad Ibrahim 
Published: March 2020

Cladosporium sp. plays an important role in human health, it is one of the pathogenic fungi which cause allergy and asthma and most frequently isolated from airborne spores. In this study, a couple of universal PCR primers were designed to identify the pathogenic fungi Cladosporium sp. according to conserved region 5.8S, 18S and 28S subunit ribosomal RNA gene in Cladosporium species. In silico RFLP-PCR were used to identify twenty-four Cladosporium strains. The results showed that the universal primer has the specificity to amplify the conserved region in 24 species as a band in virtual agarose gel. They also showed that the RFLP method is able to identify three Cladosporium species by specific and unique restriction enzymes for each one. These species are Cl. halotorenas by the two unique enzymes BsaXI and MobII, the other species is Cl. colrandse by two enzymes BccI and BtsCI, while the third species is Cl. aciculare by one enzyme BceAI. Each enzyme forms two bands in virtual agarose gel as a results of cutting the DNA by the enzyme, where the rest twenty – two species share more than one restriction enzymes. This method is active and rapid for identifying Cladosporium genus and three species by computational bases methods before applying it in the lab for more accuracy, efficiency, and specificity of designed primer to get good results in a short time.
Representaion of Algebraic Integers as Sum of Units over the Real Quadratic Fields
Authors: Baddai Saad abood 
Published: March 2020

In this paper we generalize Jacobsons results by proving that any integer in is a square-free integer), belong to . All units of are generated by the fundamental unit having the forms
Our generalization build on using the conditions
This leads us to classify the real quadratic fields into the sets Jacobsons results shows that and Sliwa confirm that and are the only real quadratic fields in .
Solutions and Recent Challenges Related to Energy in Wireless Body Area Networks with Integrated Technologies: Applications and Perspectives
Authors: S.aldeen Yousra abdul alsahib , Qureshi Kashif Naseer 
Published: March 2020

In this paper, we have investigated some of the most recent energy efficient routing protocols for wireless body area networks. This technology has seen advancements in recent times where wireless sensors are injected in the human body to sense and measure body parameters like temperature, heartbeat and glucose level. These tiny wireless sensors gather body data information and send it over a wireless network to the base station. The data measurements are examined by the doctor or physician and the suitable cure is suggested. The whole communication is done through routing protocols in a network environment. Routing protocol consumes energy while helping non-stop communication in a wireless environment. Because of the very tiny size, energy consumption matters a lot. We have focused on the protocols, which provide energy efficiency and improve network lifetime, through put and minimal path loss. We will be discussing some of the most recent energy efficient routing protocols in this paper; later their comparison with an appropriate table is presented. In addition, recent challenges, possible application and perspectives are discussed.
Software Defined Network of Video Surveillance System Based on Enhanced Routing Algorithms
Authors: Salman et al. Mustafa I. 
Published: March 2020

Software Defined Network (SDN) is a new technology that separate the ‎control plane from the data plane. SDN provides a choice in automation and ‎programmability faster than traditional network. It supports the ‎Quality of Service (QoS) for video surveillance application. One of most ‎significant issues in video surveillance is how to find the best path for routing the packets ‎between the source (IP cameras) and destination (monitoring center). The ‎video surveillance system requires fast transmission and reliable delivery ‎and high QoS. To improve the QoS and to achieve the optimal path, the ‎SDN architecture is used in this paper. In addition, different routing algorithms are ‎used with different steps. First, we evaluate the video transmission over the SDN with ‎Bellman Ford algorithm. Then, because the limitation of Bellman ford ‎algorithm, the Dijkstra algorithm is used to change the path when a congestion occurs. Furthermore, the Dijkstra algorithm is used with two ‎controllers to reduce the time consumed by the SDN controller. ‎ POX and Pyretic SDN controllers are used such that POX controller is ‎responsible for the network monitoring, while Pyretic controller is responsible for the ‎routing algorithm and path selection. Finally, a modified Dijkstra algorithm is further proposed and evaluated with two ‎controllers to enhance the performance. The results show that the modified Dijkstra algorithm outperformed the other approaches in the aspect of QoS parameters.
Adapting a nurse-led primary care initiative to cardiovascular disease control in Ghana: A qualitative study
Authors: Haykin Leah A. , Francke Jordan A. , Abapali Aurelia , Yakubu Elliasu , Dambayi Edith , Jackson Elizabeth , Aborigo Raymond , Awuni Denis , Nonterah Engelbert A. , Oduro Abraham R. , Bawah Ayaga A , Phillips James F. , Heller David J 
Published: March 2020

Abstract
Background : Cardiovascular Disease (CVD) is a growing cause of morbidity and mortality in Ghana, where rural primary health care is provided mainly by the Community-based Health Planning and Services (CHPS) initiative. CHPS locates nurses in community-level clinics for basic curative and preventive health services and provides home and outreach services. But CHPS currently lacks capacity to screen for or treat CVD and its risk factors. Methods : In two rural districts, we conducted in-depth interviews with 21 nurses and 10 nurse supervisors to identify factors constraining or facilitating CVD screening and treatment. Audio recordings were transcribed, coded for content, and analyzed for key themes. Results : Respondents emphasized three themes: community demand for CVD care; community access to CVD care; and provider capacity to render CVD care. Nurses and supervisors noted that community members were often unaware of CVD, despite high reported prevalence of risk factors. Community members were unable to travel for care or afford treatment once diagnosed. Nurses lacked relevant training and medications for treating conditions such as hypertension. Respondents recognized the importance of CVD care, expressed interest in acquiring further training, and emphasized the need to improve ancillary support for primary care operations. Conclusions : CHPS staff expressed multiple constraints to CVD care, but also cited actions to address them: CVD-focused training, provision of essential equipment and pharmaceuticals, community education campaigns, and referral and outreach transportation equipment. Results attest to the need for trial of these interventions to assess their impact on CVD risk factors such as hypertension, depression, and alcohol abuse.
The clinical significance of IL-6s and IL-27s in Bronchoalveolar lavage fluids from children with mycoplasma pneumoniae pneumonia
Authors: Zhao Jie , Li Yuyun , Zhang Wen 
Published: March 2020

Abstract
Backgrounds: IL-6 is associated with the severity of mycoplasma pneumoniae pneumonia (MPP). But the relationship between IL-27 and MPP was unknown. Methods: 98 patients with MPP who were under 14 years old, were enrolled in this study and divided into mild cases and severe cases, Mycoplasma pneumoniae (MP) single infection group and MP mixed infection group, and low MP DNA loads group and high MP DNA loads group, by severity, infection types and DNA loads respectively. 15 children with foreign bodies for bronchus were also enrolled as control. IL-6s and IL-27s in bronchoalveolar lavage fluids (BALFs) from these children were measured by ELISA. Results: There were significant differences in IL-6s of BALFs from patients between mild cases and severe cases, MP single infection group and MP mixed infection group, and low MP DNA loads group and high MP DNA loads group, respectively (P﹤0.05). Compared with IL-6s of BALFs from control, IL-6 in BALFs from the 5 patient groups were significantly higher (P﹤0.05) except that from low MP DNA loads group. IL-27s in BALFs from MP mixed infection group were significantly lower than those from MP single infection group and control (P﹤0.05) respectively. Conclusion: IL-6 was firmly associated with MPP and had potential application in clinical practice while IL-27 was not related to MP infection.
The difficulties experienced during the preparation and administration of oral drugs by parents at home: a cross-sectional study from Palestine
Authors: Ali Ra'fat , Shadeed Abdullah , Fitian Hasan , Zyoud Sa'ed 
Published: March 2020

Abstract
Background: Failure to properly administer drugs to children at home may cause adverse events, which makes it a challenging job for parents or caregivers. The main goal of this study was to investigate the problems and difficulties that parents or caregivers faced when administering oral drugs to their children at home.

Methods: A cross-sectional study was conducted using a questionnaire consisting of ‘yes/no’ and multiple-response questions to assess parents' experiences and problems with administering medication to their children at home. Data was collected from parents who visited primary health care centres in Nablus. Descriptive analysis was conducted to describe the characteristics of the sample.

Results: We interviewed 420 participants. 91.9% of the participants used drugs without prescription from a doctor, and the most commonly used non-prescription medicines were antipyretics (n=386, 100%), influenza drugs (n=142, 36.8%), cough drugs (n=109, 28.2%) and antibiotics (n= 102, 26.4%). The study showed that 21.7% of parents used teaspoon and 7.1% used tablespoon in administering liquid medications to their children. When the children refused taking liquid medications, almost two-thirds of the participants (65.7%) insisted their children take them, 21.5% mixed it with juice, 5.2% mixed it with food and 4.7% mixed it with milk. 12.4% of the participants reported that they gave drugs in doses higher than prescribed by the doctor to treat their children more quickly. Also, our study revealed that 80.5% of the parents gave medications at incorrect intervals.

Conclusions: This study has shown that there is a proportion of caregivers or parents who administer oral drugs to their children wrongly, which may involve giving them at the wrong intervals or doses, using incorrect instruments, or mixing them with food, juice or milk. The development of educational programs that will provide parents with education about medication administration is therefore recommended.
The frequency of reduction loss after arthroscopic fixation of acute acromioclavicular dislocations using a double-button device, and its effect on clinical and radiological results
Authors: Çarkçı Engin , polat ayşe e , Gürpınar Tahsin 
Published: March 2020

Abstract
Background

The aim of this study was to investigate the effect of reduction loss of more than 3 mm on clinical and radiological results after at least 2 years of follow-up after arthroscopic fixation of acute acromioclavicular joint dislocations using a double-button device.

Methods

Thirty-six patients who had acute (<3 weeks old), type III or V Acromioclavicular (AC) joint dislocations underwent arthroscopic fixation of the AC joint using a double-button device. Clinical and radiological evaluations were performed at preoperative, postoperative first day, 3 months and last follow-up. When the coracoclavicular (CC) distances of patients at the last follow-up were compared to the early postop CC distances, those with a difference of 3 mm or less were grouped as group A and those with a difference of more than 3 mm were grouped as group B.

Results

There was no statistically significant difference between the groups in terms of age, gender, follow-up time, time from injury to surgery, return to work, and distribution of Rockwood classification. Pre-operative CC distance was reduced from 18.7 ± 3.5 to 8.5 ± 0.6 in the early postoperative period. Anatomic reduction was achieved in all patients compared with the unaffected side (CC distance 8.6 ± 0.7). However, the CC distance increased to 9.9 ± 1.5 at the third month follow-up and increased to 11 ± 2.7 at the last follow-up. There were no significant Constant score differences between the groups in the preoperative and last follow-up periods (p>0.05). At the last follow-up, the mean Acromioclavicular Joint Instability (ACJI) score of group A was 84.4 ± 8, whereas it was 68.3 ± 8.3 for group B, and the difference was statistically significant (p<0.01). Furthermore, the subjective evaluation and aesthetic subjective satisfaction values of group B were lower than group A (p <0.01).

Conclusions

Reduction loss of more than 3 mm was observed in 25% of patients after arthroscopic fixation of acute acromioclavicular dislocations using a double-button device. Although this loss did not create a statistically significant difference in Constant scores, AC joint-specific tests such as ACJI, subjective evaluation, and aesthetic subjective satisfaction values were significantly impaired.
The toxicity of lithium to human cardiomyocytes
Authors: Shen Junwei , Li Xin , Shi Xiujuan , Wang Wei , Zhou Hua , Wu Jiawen , Wang Xin , Li Jue 
Published: March 2020

Abstract
Background: Lithium is widely used in the electronic consumer market and electric vehicles and has a great contribution in the world economy, resulting in large quantities of lithium waste in the environment. Yangtze River basin is one of the most developed areas in China. However, the environment influence of lithium in the Yangtze River basin and its roles in cardiomyocytes has not yet been clarified.
Results: Here we found that the concentration of lithium in the water environment is very high in Shanghai, as well as in tap water, which might be cause by the pollution of lithium batteries. Lithium inhibits not only cell viability of human cardiomyocytes, but also cell proliferation. Moreover, lithium promotes cell apoptosis of cardiomyocytes significantly. And we found that lithium controls cardiomyocytes' functions through regulating Gsk3β signaling.
Conclusions: This study reveals that the water environment of Shanghai might be polluted by the lithium batteries; and the enrichment of lithium may cause damage to human cardiomyocytes; and it is imperative to detect lithium concentration in the water environments (such as tap water and irrigation water) and effectively recycle lithium batteries in the future.
Multiplex and On-site PCR detection of swine diseases based on the microfluidic chip system
Authors: Jiang Yan , Jiang Shan , Wu Yue , Zhou Bin , Wang Kaiming , Jiang Luyan , Long Yunfeng , Chen Gan , Zeng Dexin 
Published: March 2020

Abstract
Background: At present, the process of inspection and quarantine starts with sampling at the customs port, continues with transporting the samples to the central laboratory for inspection experiments, and ends with the inspected results being fed back to the port. This process takes a rather long time, has the risks of degradation of biological samples and generation of pathogenic microorganisms, and does not meet the rapid on-site detection demand. Therefore, development of a technology for rapid and high-throughput detection of pathogenic microorganisms at the customs port is of great significance. This study was to develop a microfluidic chip to be applied to rapid high-throughput detection for swine disease with higher accuracy and lower risk of spreading pathogenic microorganisms during transportation. Results: PCR technology has the advantages of high accurate and sensitivity in disease detection, clinical testing and food quarantine, so it plays an important role in customs inspection. However, the traditional PCR detection instrument has a large size, is time-consuming and has strict requirements on the experimental environment, which greatly limit its application in on-site testing. In this paper, the positive nucleic acid of four swine diseases were detected by a portable and rapid microfluidic PCR system, which could achieve a on-site real-time quantitative PCR detection. Eight clinical samples were detected together on the microfluidic chip in the system, and the detection results were obtained in about an hour. The detection limit of this microfluidic PCR detection system was as low as 1 copies/μL. The results show that the high sensitivity and specificity of the microfluidic PCR detection system in disease detection will play an important role in customs inspection and quarantine during customs clearance. Conclusion: The microfluidic PCR detection system established in this study could meet the requirements for rapid detection of samples at the customs port The new method can avoid the risky process of transporting the samples from the sampling site to the testing lab, and drastically reducing the inspection cycle, and would enable parallel inspections on one chip which greatly raising the efficiency of inspection.
An effective frequency-domain feature of atrial fibrillation based on time-frequency analysis
Authors: Hu Yusong , Zhao Yantao , Liu Jihong , Pang Jin , Zhang Chen , Li Peizhe 
Published: March 2020

Abstract
Background: Atrial fibrillation is a type of persistent arrhythmia that can lead to serious complications. Therefore, accurate and quick detection of atrial fibrillation by surface electrocardiogram has great importance on further treatment. The practical electrocardiogram signals contain various interferences in different frequencies, such as myoelectricity interference, power interference and so on. Detection speed and accuracy largely depend on the atrial fibrillation signal features extracted by the algorithm. But some of the discovered atrial fibrillation features are not well distinguishable, resulting in poor classification effect.
Methods: This paper proposed a high distinguishable frequency feature - the frequency corresponding to the maximum amplitude in the frequency spectrum. We used the R-R interval detection method optimized with the mathematical morphology method and combined with the wavelet transform method for analysis. According to the two features - the maximum amplitude in the frequency spectrum and R-R interval irregular, we could recognize atrial fibrillation signals in electrocardiogram signals by decision tree classification algorithm.
Results: The data used in the experiment come from the MIT-BIH database, which is publicly accessible via the web and with ethical approval and consent. Based on the input of time-domain and frequency-domain features, we classified sinus rhythm signals and AF signals using the decision tree generated by classification and regression tree (CART) algorithm. From the confusion matrix, we got the accuracy was 98.9%, sensitivity was 97.93% and specificity was 99.63%.
Conclusions: The experimental results can prove the validity of the maximum amplitude in the frequency spectrum and the practicability and accuracy of the detection method, which applied this frequency-domain feature. Through the detection method, we obtained good accuracy of classifying sinus rhythm signals and atrial fibrillation signals. And the sensitivity and specificity of our method were pretty good by comparison with other studies.
Application of preoperative assessment of pain induced by venous cannulation in predicting postoperative pain in patients under laparoscopic nephrectomy: a prospective observational study
Authors: Peng Fei , Li Yanshuang , Ai Yanqiu , Yang Jianjun , Wang Yanping 
Published: March 2020

Abstract
Background: Postoperative pain is the most prominent concern among surgical patients. It has previously been reported that venous cannulation-induced pain (VCP) can be used to predict postoperative pain after laparoscopic cholecystectomy within 90 mins in the recovery room. Its potential in predicting postoperative pain in patients with patient-controlled intravenous analgesia (PCIA) is worth establishing. The purpose of this prospective observational study was to investigate the application of VCP in predicting postoperative pain in patients with PCIA during the first 24 h after laparoscopic nephrectomy.Methods: 120 patients scheduled for laparoscopic nephrectomy were included in this study. A superficial vein on the back of the hand was cannulated with a standard-size peripheral venous catheter (1.1×3.2 mm) by a nurse in the preoperative areas.Then the nurse recorded the VAS score associated with this procedure estimated by patients, and dichotomized the patients into low response group (VAS scores < 2.0) or high response group (VAS scores ≥ 2.0). After general anesthesia and surgery, all the patients received the patient-controlled intravenous analgesia (PCIA) with sufentanil. The VAS scores at rest and on coughing at 2 h, 4 h, 8 h, 12 h, 24 h, the effective number of presses and the number of needed rescue analgesia within 24 h after surgery were recorded. Results: Peripheral venous cannulation-induced pain score was significantly correlated with postoperative pain intensity at rest (rs = 0.64) and during coughing (rs = 0.65), effective times of pressing (rs = 0.59), additional consumption of sufentanil (rs = 0.58). Patients with venous cannulation-induced pain intensity ≥ 2.0 VAS units reported higher levels of postoperative pain intensity at rest (P < 0.0005) and during coughing (P < 0.0005), needed more effective times of pressing (P < 0.0005) and additional consumption of sufentanil (P < 0.0005), and also needed more rescue analgesia (P = 0.01) during the first 24 h. The odds of risk for moderate or severe postoperative pain (OR 3.5, 95% CI 1.3-9.3) was significantly higher in patients with venous cannulation-induced pain intensity ≥ 2.0 VAS units compared to those < 2.0 VAS units. Conclusions: Preoperative assessment of pain induced by venous cannulation can be used to predict postoperative pain intensity in patients with PCIA during the first 24 h after laparoscopic nephrectomy.Trial registration: We registered this study in a Chinese Clinical Trial Registry (ChiCTR) center on July 6 2019 and received the registration number: ChiCTR1900024352.