bluuebunny/crossref_metadata_2025_split huggingface dataset:
A randomized double-blind trial of 3 aspirin regimens to optimize antiplatelet therapy in essential thrombocythemia
Authors: Rocca Bianca , Tosetto Alberto , Betti Silvia , Soldati Denise , Petrucci Giovanna , Rossi Elena , Timillero Andrea , Cavalca Viviana , Porro Benedetta , Iurlo Alessandra , Cattaneo Daniele , Bucelli Cristina , Dragani Alfredo , Di Ianni Mauro , Ranalli Paola , Palandri Francesca , Vianelli Nicola , Beggiato Eloise , Lanzarone Giuseppe , Ruggeri Marco , Carli Giuseppe , Elli Elena Maria , Carpenedo Monica , Randi Maria Luigia , Bertozzi Irene , Paoli Chiara , Specchia Giorgina , Ricco Alessandra , Vannucchi Alessandro Maria , Rodeghiero Francesco , Patrono Carlo , De Stefano Valerio 
Published: April 2020

Abstract
Essential thrombocythemia (ET) is characterized by abnormal megakaryopoiesis and enhanced thrombotic risk. Once-daily low-dose aspirin is the recommended antithrombotic regimen, but accelerated platelet generation may reduce the duration of platelet cyclooxygenase-1 (COX-1) inhibition. We performed a multicenter double-blind trial to investigate the efficacy of 3 aspirin regimens in optimizing platelet COX-1 inhibition while preserving COX-2–dependent vascular thromboresistance. Patients on chronic once-daily low-dose aspirin (n = 245) were randomized (1:1:1) to receive 100 mg of aspirin 1, 2, or 3 times daily for 2 weeks. Serum thromboxane B2 (sTXB2), a validated biomarker of platelet COX-1 activity, and urinary prostacyclin metabolite (PGIM) excretion were measured at randomization and after 2 weeks, as primary surrogate end points of efficacy and safety, respectively. Urinary TX metabolite (TXM) excretion, gastrointestinal tolerance, and ET-related symptoms were also investigated. Evaluable patients assigned to the twice-daily and thrice-daily regimens showed substantially reduced interindividual variability and lower median (interquartile range) values for sTXB2 (ng/mL) compared with the once-daily arm: 4 (2.1-6.7; n = 79), 2.5 (1.4-5.65, n = 79), and 19.3 (9.7-40; n = 85), respectively. Urinary PGIM was comparable in the 3 arms. Urinary TXM was reduced by 35% in both experimental arms. Patients in the thrice-daily arm reported a higher abdominal discomfort score. In conclusion, the currently recommended aspirin regimen of 75 to 100 once daily for cardiovascular prophylaxis appears to be largely inadequate in reducing platelet activation in the vast majority of patients with ET. The antiplatelet response to low-dose aspirin can be markedly improved by shortening the dosing interval to 12 hours, with no improvement with further reductions (EudraCT 2016-002885-30).
Investigation of donor KIR content and matching in children undergoing hematopoietic cell transplantation for acute leukemia
Authors: Verneris Michael R. , Miller Jeffrey S. , Hsu Katherine C. , Wang Tao , Sees Jennifer A. , Paczesny Sophie , Rangarajan Hemalatha , Lee Dean A. , Spellman Stephen R. , Lee Stephanie J. 
Published: April 2020

Abstract
Multiple models of donor killer immunoglobulin receptor (KIR) alloreactivity or KIR genotype have been reported to be protective against leukemia relapse after allogeneic transplantation. However, few studies have addressed this topic in the pediatric population. Here, we assessed the outcomes of allogeneic transplantation in children with acute lymphoblastic leukemia (ALL; n = 372) or acute myeloid leukemia (AML; n = 344) who received unrelated donor (URD) transplantation and were reported to the Center for International Blood and Marrow Transplant Research (CIBMTR) from 2005 to 2016. As expected in this pediatric population, most patients underwent myeloablative conditioning while in remission and with bone marrow as a stem cell source. We tested KIR ligand mismatch, KIR gene content (centromeric [Cen] B), KIR2DS1 mismatching, and Cen B/telomeric A using Cox regression models and found that none were significantly associated with either relapse or disease-free survival when considering the entire cohort of patients (ALL and AML), AML, or ALL separately. Moreover, there was no significant association with outcomes in the in vivo T-cell–depleted (ie, serotherapy) cohort. This study, which is the largest analysis of donor KIR in the pediatric acute leukemia population, does not support the use of KIR in the selection of URDs for children undergoing T-replete transplantation.
LAG3: a novel immune checkpoint expressed by multiple lymphocyte subsets in diffuse large B-cell lymphoma
Authors: Keane Colm , Law Soi C. , Gould Clare , Birch Simone , Sabdia Muhammed B. , Merida de Long Lilia , Thillaiyampalam Gayathri , Abro Emad , Tobin Joshua W. , Tan Xiaohong , Xu-Monette Zijun Y. , Young Ken H. , Gifford Grace , Gabreilli Sara , Stevenson William S. , Gill Anthony , Talaulikar Dipti , Jain Sanjiv , Hernandez Annette , Halliday Sarah-Jane , Bird Robert , Cross Donna , Hertzberg Mark , Gandhi Maher K. 
Published: April 2020

AbstractBlockade of the PD-1 axis has modest efficacy in diffuse large B-cell lymphoma (DLBCL), but data regarding LAG3 are sparse. The impact of LAG3 digital gene expression was tested in 309 patients with DLBCL treated with standard chemoimmunotherapy. Cellular distribution of LAG3 protein was determined by immunohistochemistry and flow cytometry. In tumor-infiltrating lymphocytes (TILs), LAG3 expression was highest on CD4+ regulatory T cells (Tregs) and was also highly expressed on CD8+ T cells compared with CD4+ non-Tregs (both P = .008). LAG3high TILs were enriched in PD-1 and TIM-3. LAG3 was also expressed on a proportion of malignant B cells, and these patients had significantly higher LAG3 messenger RNA in their biopsies (P = .03). LAG3high gene expression was associated with inferior survival in discovery/validation cohorts, independent of cell of origin and the international prognostic index. Patients who were PD-L1high were fivefold more likely to be LAG3high (P &lt; .0001). Patients who were LAG3high/PD-L1high had an inferior progression-free survival (P = .011) and overall survival (P = .005) compared with patients who were LAG3low/PD-L1high. Digital spatial protein analysis confirms LAG3 expression on T cells and, surprisingly, tumor-associated macrophages (TAMs) at higher levels than found on CD20+ B cells in the tumor microenvironment. LAG3 is frequently expressed on CD4+ Tregs and CD8+ TILs, typically with other immune checkpoints, and is also present in a proportion of malignant B cells in DLBCL and in areas enriched for TAMs. LAG3high expression is associated with poor outcome independent of conventional prognosticators.
Low red/far-red ratio as a signal promotes carbon assimilation of soybean seedlings by increasing the photosynthetic capacity
Authors: Yang Feng , Liu Qinlin , Cheng Yajiao , Feng Lingyang , Wu Xiaoling , Fan Yuanfang , Raza Muhammad Ali , Wang Xiaochun , Yong Taiwen , Liu Weiguo , Liu Jiang , Du Junbo , Shu Kai , Yang Wenyu 
Published: April 2020

Abstract
Background
Shading includes low light intensity and varying quality. However, a low red/far-red (R/Fr) ratio of light is a signal that affects plant growth in intercropping and close- planting systems. Thus, the low R/Fr ratio uncoupling from shading conditions was assessed to identify the effect of light quality on photosynthesis and CO2 assimilation. Soybean plants were grown in a growth chamber with natural solar radiation under four treatments, that is, normal (N, sunlight), N + Fr, Low (L) + Fr, and L light.

Results
Low R/Fr ratio significantly increased the total biomass, leaf area, starch and sucrose contents, chlorophyll content, net photosynthetic rate, and quantum efficiency of the photosystem II compared with normal R/Fr ratio under the same light level (P < 0.05). Proteomic analysis of soybean leaves under different treatments was performed to quantify the changes in photosynthesis and CO2 assimilation in the chloroplast. Among the 7834 proteins quantified, 12 showed a > 1.3-fold change in abundance, of which 1 was related to porphyrin and chlorophyll metabolism, 2 were involved in photosystem I (PS I), 4 were associated with PS II, 3 proteins participated in photosynthetic electron transport, and 2 were involved in starch and sucrose metabolism. The dynamic change in these proteins indicates that photosynthesis and CO2 assimilation were maintained in the L treatment by up-regulating the component protein levels compared with those in N treatment. Although low R/Fr ratio increased the photosynthetic CO2 assimilation parameters, the differences in most protein expression levels in N + Fr and L + Fr treatments compared with those in N treatment were insignificant. Similar trends were found in gene expression through quantitative reverse transcription polymerase chain reaction excluding the gene expression of sucrose synthase possible because light environment is one of the factors affecting carbon assimilation.

Conclusions
Low R/Fr ratio (high Fr light) can increase the photosynthetic CO2 assimilation in the same light intensity by improving the photosynthetic efficiency of the photosystems.
Salicylic acid-primed defence response in octoploid strawberry ‘Benihoppe’ leaves induces resistance against Podosphaera aphanis through enhanced accumulation of proanthocyanidins and upregulation of pathogenesis-related genes
Authors: Feng Jun , Zhang Min , Yang Kang-Ning , Zheng Cai-Xia 
Published: April 2020

Abstract
Background
Podosphaera aphanis, a predominately biotrophic fungal pathogen, causes significant yield losses of strawberry. China is the largest strawberry producer in the world, and selecting for powdery mildew-resistant cultivars is desirable. However, the resistance mechanism against P. aphanis in the octoploid strawberry remains unclear.

Results
To understand possible mechanisms of disease resistance, we inoculated strawberry leaves with P. aphanis, and examined the expression profiles of candidate genes and the biochemical phenotypes in strawberry leaves of two groups. The unigenes obtained from ddH2O- and SA-pretreated leaves resulted in a total of 48,020 and 45,896 genes, respectively. KEGG enrichment showed that phenylpropanoid biosynthesis and plant hormone signal transduction pathways were enriched to a noticeable extent. DEG analysis showed that key TFs genes associated with the SA signaling pathway could play important role in the strawberry–P. aphanis interaction. In particular, FaWRKY70, FaJAZ1 and FaMYC2-like, involved in regulating the antagonistic effect of SA and JA signaling pathway, leading to increased expression of SA-responsive genes (in particular PR1, PR2, PR3, and PR5) compared to a decline in expression of JA-responsive genes (FaJAR1, FaAOS, and FaLOX2). Furthermore, SA pretreatment induced accumulation of PAs by activating the MBW complex and inhibit powdery mildew growth.

Conclusions
This study describes the role of the proanthocyanidins (PAs), pathogenesis-related (PR) genes, SA, and transcription factors in regulatory model against P. aphanis, which coincided with an early activation of defense, leading to the accumulation of PAs and the PR proteins.
New three-dimensional positioning algorithm through integrating TDOA and Newton’s method
Authors: Qu Junsuo , Shi Haonan , Qiao Ning , Wu Chen , Su Chang , Razi Abolfazl 
Published: April 2020

AbstractPassive time difference location is an important method for passive location. There are fuzzy positioning, no solution, and low positioning accuracy with the spherical coordinate conversion method in the four-station TDOA positioning algorithm. Focusing on these problems, we proposed a combination of TDOA and iterative Newton’s method. The positioning method uses the result obtained by the four-station TDOA location algorithm as the initial value of the iterative Newton’s method and solves the problem of no solution and fuzzy positioning caused by the four-station TDOA location algorithm by using the spherical coordinates conversion method. By simulating the target at a height of 5 km and traveling at a constant speed for 40 km, the positioning accuracy of the root mean square error is less than 45 m, which can achieve the same positioning accuracy of TDOA based on the least square algorithm. As the baseline length increases, the positioning accuracy is better than the least square algorithm.
Global attractivity of Leslie–Gower predator-prey model incorporating prey cannibalism
Authors: Lin Qifa , Liu Chulei , Xie Xiangdong , Xue Yalong 
Published: April 2020

AbstractLeslie–Gower predator prey model with Holling II type cannibalism term is proposed and studied in this paper. Local and global stability properties of the system are investigated. Our study indicates that cannibalism has no influence on the local stability property of the equilibrium, which is quite different to the known results. Numeric simulations are carried out to show the feasibility of the main results.
Bacterial cell division at a glance
Authors: Mahone Christopher R. , Goley Erin D. 
Published: April 2020

ABSTRACT
Bacterial cell division is initiated by the midcell assembly of polymers of the tubulin-like GTPase FtsZ. The FtsZ ring (Z-ring) is a discontinuous structure made of dynamic patches of FtsZ that undergo treadmilling motion. Roughly a dozen additional essential proteins are recruited to the division site by the dynamic Z-ring scaffold and subsequently activate cell wall synthesis to drive cell envelope constriction during division. In this Cell Science at a Glance article and the accompanying poster, we summarize our understanding of the assembly and activation of the bacterial cell division machinery. We introduce polymerization properties of FtsZ and discuss our current knowledge of divisome assembly and activation. We further highlight the intimate relationship between the structure and dynamics of FtsZ and the movement and activity of cell wall synthases at the division site, before concluding with a perspective on the most important open questions on bacterial cell division.
Do Corporate Social Responsibility Engagements Lead to Real Environmental, Social, and Governance Impact?
Authors: Li Jun , Wu Di (Andrew) 
Published: April 2020

We construct an event-based outcome measure of firm-level environmental, social, and governance (ESG) impact for public and private firms globally from 2007 to 2015 using data from RepRisk. Then we measure the societal impact of corporate social responsibility (CSR) engagements using participation in the United Nations Global Compact (UNGC) as a proxy. We demonstrate a robust and striking difference between public and private firms: whereas private firms significantly reduce their negative ESG incident levels after UNGC engagements, public firms fail to do so and are more likely to engage in decoupled CSR actions—actions with no subsequent real impact. We then conduct a series of in-depth analyses to examine possible economic mechanisms. Our results are most consistent with shareholder–stakeholder conflicts of interest being the main moderator of decoupling. The intensity of this conflict is further moderated by three factors: ownership type, proximity to final consumers on the value chain, and specific ESG incident types. Other possible mechanisms, such as selective entry into UNGC and heterogeneities in media exposure, country representation, and entry timing, do not survive our analysis. Our results suggest that existing CSR engagements and one-size-fits-all CSR policy mandates might not necessarily lead to better societal outcomes, and a multi-tiered policy targeting different ownership and industry types might be more efficient at maximizing ex post ESG benefits.This paper was accepted by Bruno Cassiman, business strategy.
A comparison of handover communication in NHS and military emergency care
Authors: Slope Rowena , Pope Catherine , Crouch Robert , Bernthal Elizabeth MM 
Published: April 2020

Background: There is a gap in the literature comparing communication during handover between military and NHS emergency care settings. Objectives: This study aimed to explore differences in handover communication in the NHS and the military, and to understand how paramedics manage the transition between settings. Design: This was a qualitative study for which 13 paramedics were interviewed. It focused on handover communication in NHS emergency care settings and Camp Bastion Hospital, Afghanistan. Methods: Interviews were conducted with regular and reservist paramedics serving in the Royal Air Force who had undertaken a deployment with the Medical Emergency Response Team. Semi-structured interviews were recorded, transcribed, coded and subjected to a thematic analysis. Results: Three principal themes were identified: differences between handover communication; standardisation; and the challenge of transition. Conclusion: Participants were most concerned about standardisation. Transition theory and resilience may account for the difficulties encountered when transitioning between different care settings.
Broadband mid-infrared (2.5–5.5  µm) emission from Co2+/Fe2+ codoped chalcogenide glass ceramics
Authors: Lu Xiaosong , Li Jianhui , Yang Lu , Ren Jing , Sun Mingyang , Yang Anping , Yang Zhiyong , Jain Ravinder Kumar , Wang Pengfei 
Published: April 2020

Compact, mechanically robust, and cost-effective mid-infrared (MIR) light sources are key components in portable and field-deployable gas sensors. Capitalizing on an efficient energy transfer mechanism between

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, we have demonstrated for the first time, to the best of our knowledge, that ultrabroadband 2.5–5.5 µm MIR emission can be achieved at room temperatures in chalcogenide (ChG) glasses that are pumped by a commercially available erbium-doped fiber amplifier emitting at 1.57 µm. These MIR-transparent ChG glass ceramics are embedded with

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codoped ZnSe nanocrystals, and show sufficient MIR emission intensities and bandwidths to enable gas sensing for multiple target analytes such as butane and carbon dioxide. We also describe, to the best of our knowledge, the first observation of a unique “anomalous” increase in the MIR luminescence intensity as a function of temperature.
Aberration-free 3D imaging via DMD-based two-photon microscopy and sensorless adaptive optics
Authors: Ren Mindan , Chen Jialong , Chen Dihan , Chen Shih-Chi 
Published: April 2020

In this Letter, we present a new, to our knowledge, aberration-free 3D imaging technique based on digital micromirror device (DMD)-based two-photon microscopy and sensorless adaptive optics (AO), where 3D random-access scanning and modal wavefront correction are realized using a single DMD chip at 22.7 kHz. Specifically, the DMD is simultaneously used as a deformable mirror to modulate a distorted wavefront and a fast scanner to maneuver the laser focus in a 3D space by designed binary holograms. As such, aberration-free 3D imaging is realized by superposing the wavefront correction and 3D scanning holograms. Compared with conventional AO devices and methods, the DMD system can apply optimal wavefront correction information to different imaging regions or even individual pixels without compromising the scanning speed and device resolution. In the experiments, we first focus the laser through a diffuser and apply sensorless AO to retrieve a corrected focus. After that, the DMD performs 3D scanning on a Drosophila brain labeled with green fluorescent protein. The two-photon imaging results, where optimal wavefront correction information is applied to

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separate regions, demonstrate significantly improved resolution and image quality. The new DMD-based imaging solution presents a compact, low-cost, and effective solution for aberration-free two-photon deep tissue imaging, which may find important applications in the field of biophotonics.
A Mouse Model for Studying Stem Cell Effects on Regeneration of Hair Follicle Outer Root Sheaths
Authors: Guo Jingxu , Li Shuwei , Wang Hongyang , Wu Tinghui , Wu Zhenhui , Yu Lufei , Liang Meiyan 
Published: April 2020

AbstractObjectiveStem cells hold promise for treating hair loss. Here an in vitro mouse model was developed using outer root sheaths (ORSs) isolated from hair follicles for studying stem cell-mediated dermal papillary regeneration.MethodsUnder sterile conditions, structurally intact ORSs were isolated from hair follicles of 3-day-old Kunming mice and incubated in growth medium. Samples were collected daily for 5 days. Stem cell distribution, proliferation, differentiation, and migration were monitored during regeneration.ResultsCell proliferation began at the glass membrane periphery then spread gradually toward the membrane center, with the presence of CD34 and CD200 positive stem cells involved in repair initiation. Next, CD34 positive stem cells migrated down the glass membrane, where some participated in ORS formation, while other CD34 cells and CD200 positive cells migrated to hair follicle centers. Within the hair follicle matrix, stem cells divided, grew, differentiated and caused outward expansion of the glass membrane to form a dermal papillary structure containing alpha-smooth muscle actin. Neutrophils attracted to the wound site phagocytosed bacterial and cell debris to protect regenerating tissue from infection.ConclusionIsolated hair follicle ORSs can regenerate new dermal papillary structuresin vitro. Stem cells and neutrophils play important roles in the regeneration process.
User-centered feedback design in person-following robots for older adults
Authors: Olatunji Samuel , Oron-Gilad Tal , Sarne-Fleischmann Vardit , Edan Yael 
Published: April 2020

AbstractFeedback design is an important aspect in person-following robots for older adults. This paper presents a user-centered design approach to ensure the design is focused on users’ needs and preferences. A sequence of user studies with a total of 35 older adults (aged 62 years and older) was conducted to explore their preferences regarding feedback parameters for a socially assistive person-following robot. The preferred level of robot transparency and the desired content for the feedback was first explored. This was followed by an assessment of the preferred mode and timing of feedback. The chosen feedback parameters were then implemented and evaluated in a final experiment to evaluate the effectiveness of the design. Results revealed that older adults preferred to receive only basic status information. They preferred voice feedback over tone, and at a continuous rate to keep them constantly aware of the state and actions of the robot. The outcome of the study is a further step towards feedback design guidelines that could improve interaction quality in person-following robots for older adults.
Particularidades da educação física na pré-escola em três municípios da região do Grande ABC PaulistaIÃO DO GRANDE ABC PAULISTA
Authors: Moura Sobrinho Alex , Garcia Paulo Sergio 
Published: April 2020

A educação infantil é uma fase de extrema relevância para o desenvolvimento integral das crianças. É nessa etapa que elas podem vivenciar o maior número possível de experiências e, neste contexto, a Educação Física é fundamental. Todavia, essa disciplina apresenta grande variedade de propostas educacionais e pedagógicas. Este estudo analisa algumas particularidades – similaridades e diferenças – educacionais da Educação Física na pré-escola para o atendimento das crianças em três municípios da região do Grande ABC. Nesta pesquisa foi utilizada a metodologia qualitativa, a partir da análise de documentos e de entrevistas semiestruturadas com os diretores escolares. Os resultados revelaram preocupações e zelo das autoridades políticas e educacionais. As cidades possuíam diretrizes curriculares que orientavam o trabalho dos professores e os diretores possuíam argumentações sólidas quanto à importância da Educação Física. Todavia também foram encontrados elementos de descaso em relação à formação do professor que ministra a aula e à infraestrutura escolar. Os dados deste estudo podem ser utilizados no contexto da formação inicial e contínua de professores e, ao mesmo tempo, pelas secretarias de educação dos municípios brasileiros.