bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ecological stoichiometric characteristics of Carbon (C), Nitrogen (N) and Phosphorus (P) in leaf, root, stem, and soil in four wetland plants communities in Shengjin Lake, China
Authors: Dibar Dagne Tafa , Zhang Kun , Yuan Suqiang , Zhang Jinyu , Zhou Zhongze , Ye Xiaoxin 
Published: February 2020

AbstractEcological stoichiometric should be incorporated into management and nutrient impacted ecosystems dynamic to understand the status of ecosystems and ecological interaction. The present study focused on ecological stoichiometric characteristics of different macrophyte plants soil, leave, stem and root after the removal of seine fishing since 2000 from Shengjin Lake. For C, N and P analysis from leaves, stems, roots and soil to explore their stoichiometric ratio and deriving environmental forces here four dominant plant communities (Zizania caduciflora, Vallisineria natans, Trapa quadrispinosaandCarex schmidtii) were collected. C, N, P and C: N: P ratio in leafs, stems, roots and soil among the plant communities vary and the studied plant communities had significant effect on the measured variables. There was high C: N inC.schmidtiisoil (7.08±1.504) but not vary significantly (P>0.05), and N: P ratio measured high inV. natans(13.7±4.05) and C: P inT.quadrispinosa soil(81.14±43.88) and showed significant variation (P<0.05) respectively. High leaf C: N and N: P ratio was measured inC. schmidtiiandV. natansrespectively. Nevertheless, high leaf C: P ratio was measured inZ.caduciflora. From the three studied organs leafs C: N, N: P ratio showed high values compared to root and stems. The correlation analysis result showed that, at 0-10cm depth ranges SOC correlated negatively with stem total phosphorus (STP), and RTN (P<0.05) but positively strongly with LTP and LTN (P<0.01) respectively. Soil total nitrogen at 0-10cm strongly positively correlated with LTP (P<0.01) and positively with RN: P and LTC (P<0.05). Soil basic properties such as SMC.BD and pH positively correlated with soil ecological stoichiometric characteristics. Redundancy analysis (RDA) result showed pH and available phosphorus were the potential determinant of soil stoichiometry.
Inflammatory state of lymphatic vessels and miRNA profiles associated with relapse in ovarian cancer patients
Authors: Johnson Sarah C , Chakraborty Sanjukta , Drosou Anastasios , Cunnea Paula , Tzovaras Dimitrios , Nixon Katherine , Zawieja David C , Muthuchamy Mariappan , Fotopoulou Christina , Moore James E 
Published: February 2020

AbstractLymphogenic spread is associated with poor prognosis in epithelial ovarian cancer (EOC), yet little is known regarding roles of non-peri-tumoural lymphatic vessels (LVs) outside the tumour microenvironment that may impact relapse. The aim of this feasibility study was to assess whether inflammatory status of the LVs and/or changes in the miRNA profile of the LVs have potential prognostic and predictive value for overall outcome and risk of relapse. Samples of macroscopically normal human lymph LVs (n=10) were isolated from the external iliac vessels draining the pelvic region of patients undergoing debulking surgery. This was followed by quantification of the inflammatory state (low, medium and high) and presence of cancer-infiltration of each LV using immunohistochemistry. LV miRNA expression profiling was also performed, and analysed in the context of high versus low inflammation, and cancer-infiltrated versus non-cancer-infiltrated. Results were correlated with clinical outcome data including relapse with an average follow-up time of 13.3 months. The presence of a high degree of inflammation correlated significantly with patient relapse (p=0.033). Cancer-infiltrated LVs showed a moderate but non-significant association with relapse (p=0.07). Differential miRNA profiles were identified in cancer-infiltrated LVs and those with high versus low inflammation. In particular, several members of the let-7 family were consistently down-regulated in highly inflamed LVs (>1.8-fold, p<0.05) compared to the less inflamed ones. Down-regulation of the let-7 family appears to be associated with inflammation, but whether inflammation contributes to or is an effect of cancer-infiltration requires further investigation.
The Intergenic Small Non-Coding RNAittAis Required for Optimal Infectivity and Tissue Tropism inBorrelia burgdorferi
Authors: Medina-Pérez Diana N. , Wager Beau , Troy Erin , Gao Lihui , Norris Steven J. , Lin Tao , Hu Linden , Hyde Jenny A. , Lybecker Meghan , Skare Jon T. 
Published: February 2020

ABSTRACTPost-transcriptional regulation via small regulatory RNAs (sRNAs) has been implicated in diverse regulatory processes in bacteria, including virulence. One class of sRNAs, termedtrans-acting sRNAs, can affect the stability and/or the translational efficiency of regulated transcripts. In this study, we utilized a collaborative approach that employed data from infection with theBorrelia burgdorferiTn library, coupled with Tn-seq, together with borrelial sRNA and total RNA transcriptomes, to identify an intergenictrans-acting sRNA, which we designate here asittAforinfectivity-associated andtissue-tropic sRNA locusA. The genetic inactivation ofittAresulted in a significant attenuation in infectivity, with decreased spirochetal load in ear, heart, skin and joint tissues. In addition, theittAmutant did not disseminate to peripheral skin sites or heart tissue, suggesting a role forittAin regulating a tissue-tropic response. RNA-Seq analysis determined that 19 transcripts were differentially expressed in theittAmutant relative to its genetic parent, includingvraA, bba66,ospDandoms28(bba74). Subsequent proteomic analyses also showed a significant decrease of OspD and Oms28 (BBA74) proteins. To our knowledge this is the first documented intergenic sRNA that alters the infectivity potential ofB. burgdorferi.AUTHOR SUMMARYLyme disease is a tick-borne infection mediated by the spirochetal bacterium,Borrelia burgdorferi, that is responsible for greater than 300,000 infections in the United States per year. As such, additional knowledge regarding how this pathogen modulates its regulatory armamentarium is needed to understand howB. burgdorferiestablishes and maintains infection. The identification and characterization of small, non-coding RNA molecules in living systems, designated as sRNAs, has recalibrated how we view post-transcriptional regulation. Recently, over 1,000 sRNAs were identified inB. burgdorferi. Despite the identification of these sRNAs, we do not understand how they affect infectivity orB. burgdorferipathogenesis related outcomes. Here, we characterize theittA B. burgdorferisRNA and show that it is essential for optimal infection using murine experimental infection as our readout. We also track the effect of this sRNA on the transcriptional and proteomic profile as the first step in providing mechanistic insight into how this important sRNA mediates its regulatory effect.
ERECTA family signaling constrainsCLAVATA3andWUSCHELto the center of the shoot apical meristem
Authors: Zhang Liang , DeGennaro Daniel , Lin Guangzhong , Chai Jijie , Shpak Elena D. 
Published: February 2020

AbstractThe shoot apical meristem (SAM) is a reservoir of stem cells that gives rise to all post-embryonic aboveground plant organs. The size of the SAM remains stable over time due to a precise balance of stem cell replenishment versus cell incorporation into organ primordia. The WUSCHEL (WUS)/CLAVATA (CLV) negative feedback loop is central to SAM size regulation. Its correct function depends on accurate spatial expression ofWUSandCLV3. A signaling pathway, consisting of ERECTA family (ERf) receptors and EPIDERMAL PATTERNING FACTOR LIKE (EPFL) ligands, restricts SAM width and promotes leaf initiation. While ERf receptors are expressed throughout the SAM, EPFL ligands are expressed in its periphery. Our genetic analysis demonstrated that ERfs and CLV3 synergistically regulate the size of the SAM, andwusis epistatic toerfs. Furthermore, activation of ERf signaling with exogenous EPFLs resulted in a rapid decrease ofCLV3andWUSexpression. ERf-EPFL signaling inhibits expression ofWUSandCLV3in the periphery of the SAM, confining them to the center. These findings establish the molecular mechanism for stem cell positioning along the radial axis.Summary statementERf signaling restricts the width of the shoot apical meristem, a structure which generates aboveground plant organs, by inhibiting expression of two principal regulators,CLV3andWUS, at its periphery.
Single-molecule sequencing of long DNA molecules allows high contiguityde novogenome assembly for the fungus fly,Sciara coprophila
Authors: Urban John M. , Foulk Michael S. , Bliss Jacob E. , Coleman C. Michelle , Lu Nanyan , Mazloom Reza , Brown Susan J. , Spradling Allan C. , Gerbi Susan A. 
Published: February 2020

ABSTRACTThe lower Dipteran fungus fly,Sciara coprophila, has many unique biological features. For example,Sciaraundergoes paternal chromosome elimination and maternal X chromosome nondisjunction during spermatogenesis, paternal X elimination during embryogenesis, intrachromosomal DNA amplification of DNA puff loci during larval development, and germline-limited chromosome elimination from all somatic cells. Paternal chromosome elimination inSciarawas the first observation of imprinting, though the mechanism remains a mystery. Here, we present the first draft genome sequence forSciara coprophilato take a large step forward in aiding these studies. We approached assembling theSciaragenome using multiple sequencing technologies: PacBio, Oxford Nanopore MinION, and Illumina. To find an optimal assembly using these datasets, we generated 44 Illumina assemblies using 7 short-read assemblers and 50 long-read assemblies of PacBio and MinION sequence data using 6 long-read assemblers. We ranked assemblies using a battery of reference-free metrics, and scaffolded a subset of the highest-ranking assemblies using BioNano Genomics optical maps. RNA-seq datasets from multiple life stages and both sexes facilitated genome annotation. Moreover, we anchored nearly half of theSciaragenome sequence into chromosomes. Finally, we used the signal level of both the PacBio and Oxford Nanopore data to explore the presence or absence of DNA modifications in theSciaragenome since DNA modifications may play a role in imprinting inSciara, as they do in mammals. These data serve as the foundation for future research by the growing community studying the unique features of this emerging model system.
Functional insights from biophysical study of TREM2 interactions with ApoE and Aβ1-42
Authors: Kober Daniel L. , Stuchell-Brereton Melissa D. , Kluender Colin E. , Dean Hunter B. , Strickland Michael R. , Steinberg Deborah F. , Nelson Samantha S. , Baban Berevan , Holtzman David M. , Frieden Carl , Alexander-Brett Jennifer , Roberson Erik D. , Song Yuhua , Brett Tom J. 
Published: February 2020

AbstractINTRODUCTIONTREM2 is an innate immune receptor expressed on myeloid cells including microglia in the brain. How TREM2 engages different ligands remains poorly understood.METHODSWe used comprehensive BLI analysis to investigate the TREM2 interactions with ApoE and monomeric amyloid beta (mAβ42).RESULTSTREM2 binding did not depend on ApoE lipidation, and there were only slight differences in affinity observed between ApoE isoforms (E4 > E3 > E2). Surprisingly, disease-linked TREM2 variants within a “basic patch” minimally impact ApoE binding. Instead, TREM2 has a unique hydrophobic surface that can bind to ApoE. This direct engagement requires the hinge region of ApoE. TREM2 directly binds mAβ42 and can potently inhibit Aβ42 polymerization, suggesting a potential mechanism for soluble TREM2 (sTREM2) in preventing AD pathogenesis.DISCUSSIONThese findings demonstrate that TREM2 has at least two separate surfaces to engage ligands and uncovers a potential function for sTREM2 in directly inhibiting Aβ polymerization.
Territory-based knowledge management in international marketing processes – the case of “Made in Italy” SMEs
Authors: Festa Giuseppe , Rossi Matteo , Kolte Ashutosh , Situm Mario 
Published: February 2020

Purpose
This study aims to analyze the territory as a distinctive factor through which the concept and practice of “Made in Italy” operates. Specifically, the study considers the role of local and sub-national entrepreneurial collaborations that preserve and enhance factors such as history, style and talent as the essence of Italian “quality” and as the pillar of Italian territorial capitalism.

Design/methodology/approach
The research examines this Italian phenomenon by investigating small and medium enterprises (SMEs) that successfully compete abroad (and also in the domestic market) with a “glocal” approach, adopting the entrepreneurial formula of industrial districts.

Findings
The results indicate that international expansion is becoming increasingly more complex (as is every growth/development strategy) but that “glocalism” could represent a potential driver for the success of internationalization strategies. Specifically, for SMEs operating in industrial districts, territorial capitalism could emerge as a unique competitive factor, because it is a component of local structural capital and global reputational capital, as in the case of “Made in Italy.”

Originality/value
In an increasingly globalized market environment, many companies look to foreign markets to maintain and expand competitive advantage and business performance. Once the companies embark on this endeavor, organizations are involved in governing and managing these networks of finance, production and communication and the distribution-related relationships that constitute globalization. The push to engage in international development is currently imperative for SMEs, which need to extend their business engagement beyond conventional local markets and identify and exploit their distinctive competitive advantage to be able to succeed. One possible way of achieving this is the close interaction with the local territories in which these enterprises reside.
Validation of corporate environmental citizenship measurement in Malaysia
Authors: Tay Lee Chin , Tan Fee Yean , Kirana Yahya Khulida , Rasli Amran 
Published: February 2020

Purpose
The purpose of this paper is to validate the corporate environmental citizenship measurement originally developed by Banerjee (2002) in the Malaysian setting.

Design/methodology/approach
A systematic sampling technique was used, with a total of 251 responses. The measurement was tested using content validity, convergent validity and discriminant validity.

Findings
The study finds that all four dimensions are highly suited for measuring corporate environmental citizenship in the construction companies in Malaysia.

Research limitations/implications
The study uses a single respondent to report on the organization’s corporate environmental citizenship. The perceptions among the respondents may differ.

Practical implications
Organizations can use the measurement for benchmarking current levels of organizations’ environmental degradation as well as identify which business areas are in need to improve environmental preservation.

Social implications
This study theoretically conceptualized corporate environmental citizenship as a multidimensional construct containing four dimensions.

Originality/value
This study contributes to the body of knowledge by validating corporate environmental citizenship measurement in the Malaysian context as measurement validation studies are scarcely found.
Sustainable fashion: current and future research directions
Authors: Mukendi Amira , Davies Iain , Glozer Sarah , McDonagh Pierre 
Published: February 2020

PurposeThe sustainable fashion (SF) literature is fragmented across the management discipline, leaving the path to a SF future unclear. As of yet, there has not been an attempt to bring these insights together or to more generally explore the question of “what is known about SF in the management literature and where could the SF field go from there?”. The purpose of this paper is to bring together the field to identify opportunities for societal impact and further research.Design/methodology/approachA systematic literature review was conducted from the first appearances of SF in the management literature in 2000 up to papers published in June 2019, which resulted in 465 included papers.FindingsThe results illustrate that SF research is largely defined by two approaches, namely, pragmatic change and radical change. The findings reveal seven research streams that span across the discipline to explore how organisational and consumer habits can be shaped for the future.Research limitations/implicationsWhat is known about SF is constantly evolving, therefore, the paper aims to provide a representative sample of the state of SF in management literature to date.Practical implicationsThis review provides decision makers with insights that have been synthesised from across the management field.Originality/valueThis review identifies knowledge gaps and informs managerial decision making in the field, particularly through serving as a foundation for further research.
Performance drivers of women-owned microcredit funded enterprises in Tanzania
Authors: Tundui Charles Stephen , Tundui Hawa Petro 
Published: February 2020

PurposeThe purpose of this paper is to investigate performance drivers of women-owned businesses that are funded primarily through microcredit. It draws on Storey’s theory of small business growth and family embeddedness axiom to examine the factors that drive the performance of businesses that are funded primarily through microcredit.Design/methodology/approachThe paper uses a cross-sectional survey that covered 208 women business owners who had access to microcredit. The authors use a logistic regression analysis to model the relationship between independent variables and enterprise performance.FindingsThe paper demonstrates that microcredit plays a significant role in business performance. The credit amount has the most significant influence on the enterprise capital base, whereas the effect on profits is insignificant. Also, owners are more likely to report growth in profits if they possess skills in business management. In addition, younger business owners and necessity entrepreneurs are more likely to report success in their businesses. Other factors that have a significant effect on business performance are product cycle, loan use and family support.Originality/valueMany women in Tanzania are entering business ownership and depend on microcredit as their primary source of capital for starting and growing their businesses. However, just a few businesses grow into small and medium-sized enterprises. For informed policy decisions, it is important that the factors influencing the performance of funded businesses are known and well understood. This understanding will help the government and development practitioners assist women in achieving business growth rates that could warrant their empowerment and poverty reduction prospects.
Minor sex trafficking of girls with disabilities
Authors: Franchino-Olsen Hannabeth , Silverstein Hannah A. , Kahn Nicole F. , Martin Sandra L. 
Published: February 2020

PurposeThe purpose of this paper is to investigate the associations between minor women’ (girls’) disability status and victimization via minor sex trafficking.Design/methodology/approachThis investigation used data from the National Longitudinal Study of Adolescent to Adult Health, a large, nationally-representative sample of in-school adolescents in the USA that began in 1994. The analysis included bivariate associations between physical disability status or low cognitive ability and minor sex trafficking among female survey respondents (n = 5,430).FindingsGirls with any disability had a higher prevalence of minor sex trafficking than their peers without disabilities. Odds of minor sex trafficking were significantly higher for those with severe physical disabilities (5.83) and for those with low cognitive abilities (4.86) compared to the odds of their peers without their respective disabilities. Results for girls with mild or moderate physical disabilities were not statistically significant compared to peers without disabilities.Social implicationsThese nationally-representative survey data reinforce the trends present in smaller populations and case study research: female adolescents with disabilities are at a heightened risk for sex trafficking. On both a national and global scale, the human rights gaps in policy and practice must be addressed to adequately reach, intervene and protect this vulnerable population.Originality/valueResearch about minor sex trafficking typically relies on small-scale surveys and/or convenience samples. This study used a nationally-representative survey to demonstrate the link between disability status and women’s experiences with minor sex trafficking.
Green human resource management
Authors: Shafaei Azadeh , Nejati Mehran , Mohd Yusoff Yusmani 
Published: February 2020

PurposeThe study aimed to provide insights on antecedent and outcome of green HRM at the organisational level and the outcome of green HRM at the individual level. It also sought to examine the mechanism through which green HRM would lead to employees’ positive outcome.Design/methodology/approachA quantitative study design using a two-study approach was employed to collect and analyse the data. For study 1, 206 hotels from Malaysia were included in analysis at the organisational level, while in study 2 at the individual level, 508 employees from different sectors provided insights through an online questionnaire. For both studies, partial least squares (PLS–SEM) was used to assess the research model.FindingsAll the proposed hypotheses were supported. Specifically, at the organisational level, organisational environmental culture is positively related to green HRM, and green HRM management positively associates with organisation's environmental performance. At the individual level, green HRM positively influences employees' job satisfaction, and meaningfulness through work is a strong mediator in this relationship.Originality/valueThis study is significant as it contributes to both theory and practice by providing fresh insights on green HRM and its antecedent and outcomes at two levels (organisational and individual) and across two economies (emerging and developed). It also sheds some light on the outcome of green HRM at the employee level which is an area that is still under-researched. By focusing on meaningfulness through work as an important factor, the study contributes to better understanding of green HRM and employees’ positive outcomes.
Agile vs traditional project management approaches
Authors: Zavyalova Elena , Sokolov Dmitri , Lisovskaya Antonina 
Published: February 2020

PurposeAgile project management methods gain increasing attention of practitioners while they often remain neglected by scholarly research. Specifically, there is little known about how performance factors of agile firms differ from those of traditional firms. Scholars argue that these factors often relate to a firm’s human resource management (HRM). This study aims to analyze and compare the HRM architectures in agile and traditional project-based organizations that lead to high firm performance.Design/methodology/approachThe authors apply fuzzy-set qualitative comparative analysis on data of 154 project-based organizations of diverse professional service industries in Russia.FindingsThis study’s findings suggest that HRM architectures of high-performance agile firms imply a broad use of ability-, motivation- and opportunity-enhancing practices and a high degree of HRM process centralization, while traditional firms adopt more diverse HRM architectures.Originality/valueBased on this study’s results, the authors stress the importance of ensuring a good fit between a company’s project management approach and HRM architecture. The revealed configurations may also provide guidance for practitioners on designing effective HRM architectures in project-based organizations.