bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impact of Oral Health Education for Migrants and Refugees by Community Health Workers: What do Participants Retain?
Authors: Ponce-Gonzalez Ileana , Cheadle Allen , Parchman Michael 
Published: August 2020

Abstract
Objectives. An important question for oral health education is whether knowledge gained during a workshop is retained and applied in daily practice. This study assessed the knowledge retention and changes in oral health practices several months after a lay-led education workshop for migrant farmworkers. Methods. Follow-up interviews were conducted with a random sample of participants 2-12 months post participation. Three Community Health Workers conducted the interviews using a structured Interview guide that included open-ended questions asking about both knowledge and behavior change.Results. A total of 33 interviews were conducted with participants. The results showed a fairly high degree of retention, in such areas as general oral health knowledge, and brushing/flossing frequency and technique.Conclusions. An interactive, lay-led oral health education program can be an effective way to increase oral health knowledge and change daily oral health practice in migrant populations.
Study on the Application of GE-E10 Ultrasound Equipment to Estimate Fetus Weight and Compare Its Accuracy With Birth Weight
Authors: Zhang Maochun , Chen Jiao , Zhang Hongwei , Guo Yuanyuan , Zhou Honggui 
Published: August 2020

Abstract
Background: To explore the application of GE-E10 four-dimensional color ultrasound equipment for estimation of fetus weight and compare its accuracy with birth weight. Methods: A total of 160 singleton and full-term pregnant women who delivered successfully in our department of obstetrics and gynecology from March 2017 to October 2019 were randomly selected as the study objects. The fetus weight was estimated by using clinically used two-parameter formula and GE-E10 four-dimensional color ultrasound multi-parameter. The accuracy of the two methods in estimating fetal weight was compared and analyzed. Results: The 160 puerpera enrolled: average age (23.64±6.12) years, average gestational weeks (38.56±2.34), average gravidity (2.35±1.02) times. The accuracy of GE-E10 multi-parameter ultrasound in estimating fetal weight and birth weight was 92.50% (148/160), which was significantly higher than that estimated by two-parameter formula: 72.50% (116/160), (P<0.05). The area under the ROC curve of fetus weight estimated by GE-E10 multi-parameter ultrasound was 0.842 (0.024-0.768), which is higher than that estimated by two-parameter formula was 0.717 (0.103-0.832), (P<0.05). Conclusion: The accuracy of GE-E10 four-dimensional color ultrasound equipment in estimation of fetus weight is high and effective, which can be used for estimating fetal macrosomia effectively and providing guidance and reference for delivery of puerpera.
Clinical Impact of Digital and Conventional PET Control Databases for Semi-Quantitative Analysis of Brain 18F-FDG Digital PET Scans
Authors: Mairal Elise , Doyen Matthieu , Rivasseau-Jonveaux Thérèse , Malaplate Catherine , Guedj Eric , Verger Antoine 
Published: August 2020

Abstract
Purpose: Digital PET cameras markedly improve sensitivity and spatial resolution of brain 18F-FDG PET images compared to conventional cameras. Our study aimed to assess whether specific control databases are required to improve the diagnostic performance of these recent advances.Methods: We analysed twenty-seven Alzheimer's Disease (AD) patients and twenty-two healthy subjects. These two groups underwent a brain 18F-FDG PET on a digital camera (Vereos, Philips®) with a Semi-Quantitative Analysis (SQA), i.e. comparisons to two age and sex matched controls acquired with a digital PET (Vereos, Philips®) and a conventional PET (Biograph 6, Siemens®) camera, at group and individual levels. Moreover, individual visual interpretation of SPM T-maps was provided for the positive diagnosis of AD by 3 experienced raters.Results: At group level, SQA using digital controls detected more marked hypometabolic areas in AD (+34 mm3 at p<0.001 uncorrected for the voxel, corrected for the cluster) than SQA using conventional controls. At the individual level, accuracy of SQA using digital controls was higher than SQA using conventional controls (82 vs. 63%, p<0.01, at p<0.005 uncorrected for the voxel, corrected for the cluster), with lower specificity (73 vs. 95%) but much higher sensitivity (89 vs. 37%). These results were confirmed by visual analysis (accuracies of 78% and 65% for digital and conventional controls respectively).Conclusion: There is an urgent need to establish specific digital PET control databases for SQA of brain 18F-FDG PET images as such databases improve the accuracy of AD diagnosis.
Construction of Immune-Related Prognostic Signatures in Acute Myeloid Leukemia
Authors: Xu Ruyi , Li Yi , Liu Yang , Qu Jianwei , Cao Wen , Zhang Enfan , He Jingsong , Cai Zhen 
Published: August 2020

Abstract
Background: Acute Myeloid Leukemia (AML) is characterized as a type of hematological malignancy with poor survival. Accumulated evidence showed that dysregulated immune activities contribute to the pathogenesis of AML and accelerate the development of chemotherapy resistance. Thus, we aimed to construct prognostic signatures based on patients’ immune features to sort out the high-risk group and to identify survival-related checkpoint molecules as potential therapeutic targets.Methods: In the current study, we developed two prognostic signatures based on immune genes and infiltrated fraction of immune cells, respectively, using a least absolute shrinkage and selection operator model, and Cox regression analysis on 415 samples obtained from TCGA and GEO databases. Results: We found the optimum strategy for predicting patients’ survival is combined using these two prognostic immune-related signatures. Through our established signatures, we classified patients into Favorable Risk group and Poor Risk group, who showed significantly different OS and DFS. We further demonstrated the checkpoint molecules’ profile in different risk groups. Conclusions: we constructed a powerful prognostic tool here to help classify high-risk patients in early-stage, who may benefit from additional immune therapies by targeting identified checkpoint molecules.
Survey of Perceptions and Educational Needs of Primary Care Providers Regarding Management of Patients with Class II and III Obesity in Ontario, Canada
Authors: Zevin Boris , Martin Mary Imelda , Dalgarno Nancy , Chan Linda , Sivapalan Nardhana , Houlden Robyn , Birtwhistle Richard , Smith Karen , Barber David 
Published: August 2020

Abstract
Background: Primary care providers (PCPs) are typically the primary contact for patients with obesity seeking medical and surgical weight loss interventions; however, previous studies suggest that fewer than 7% of eligible adult patients are referred to publically funded medical and surgical weight loss interventions (MSWLI).Methods: We performed an anonymous survey study between October 2017 and June 2018 to explore the knowledge, experiences, perceptions, and educational needs of PCPs in Southeastern Ontario in managing patients with class II and III obesity. Results: Surveys were distributed to 591 PCPs (n=538 family physicians; n=53 nurse practitioners) identified as practicing in the Southeastern Ontario and 103 (17.4%) participated. PCPs serving a rural population estimated that 14.2±10.9% of patients would qualify for MSWLI compared to 9.9±8.5% of patients of PCPs serving an urban population (p=.049). Overall, 57.5% of respondents did not feel competent prescribing MSWLI to patients with class II/III obesity, while 69.8% stated they had ‘good’ knowledge of the referral criteria for MSWLI. 22.2% of respondents were hesitant to refer patients for bariatric surgery (BS) due to concerns about postoperative surgical complications and risks associated with surgery. Only 25% of respondents were comfortable providing long-term follow up after BS, and only 39.1% had participated in continuing education on management of patients with class II/III obesity in the past five years. Conclusion: The majority of PCPs believe there is a need for additional education about MSWLI for patients with class II/III obesity. Future studies are needed to develop and compare the effectiveness of additional education and professional development around risks of contemporary BS, indications to consider referral for MSWLI, management and long-term follow-up of patients after BS.
Development and Validation of the Sorting Non-trauMatIc adoLescent knEe Pain (SMILE) Tool – a Development and Validation Study
Authors: Guldhammer Clara , Holden Sinead , Sørensen Marina Elmelund , Olesen Jens Lykkegaard , Jensen Martin Bach , Rathleff Michael Skovdal 
Published: August 2020

Abstract
Background: Despite the commonality of adolescent knee pain, there are no tools to support medical doctors to correctly diagnose knee pain. Aim: This study aimed to develop and evaluate a support tool for diagnosing the most common types of non-traumatic adolescent knee pain.Design and setting: Development and validation study in participants recruited from the general community. Method: A systematic search on Medline identified the literature on clinical tests and diagnoses of adolescent knee pain. The search was supplemented by textbooks and transformed into a diagnostic flowchart based on onset, symptoms, and pain localisation. This tool was revised based on feedback from general practitioners and experts in sports medicine. The tool was evaluated on two separate days with blinded assessors. Overall, 27 participants (aged 10-17 years) with non-traumatic knee pain were included. All participants were diagnosed by medical doctors or medical students, without and with the use of the tool. Diagnoses were compared to a golden standard (expert clinician). An interview to inform optimisations of the tool was performed with the assessors. Percentage agreement with the golden standard, and Kappa statistic for interrater reliability were calculated.Results: The final tool improved diagnostic agreement with the golden standard from 22.7% (95% CI 10.3-35.1) to 77.3% (95% CI 64.9-89.7). Inter-rater reliability increased from poor agreement k=-0.04 (95% CI, -0.12-0.04) moderate agreement k=0.56 (95% CI, 0.40-0.72).Conclusion: This simple diagnostic tool is quick to use and may assist doctors in diagnosing non-traumatic knee pain in adolescents.Trial registration: Not applicable.
Diversity and Plant Growth-Promoting Potential of (un)culturable Bacteria in the Hedera Helix Phylloplane
Authors: Stevens Vincent , Thijs Sofie , Vangronsveld Jaco 
Published: August 2020

Abstract
BackgroundAn abundant and diverse community of microorganisms naturally exists on the phylloplane, the surface of leaves. It is one of the most prevalent microbial habitats on earth and bacteria are by far the most abundant members, living in a community that is highly dynamic. To increase our knowledge about the diversity and function of microbial communities living in the phylloplane, culture-dependent and -independent approaches help us a great deal.ResultsHere we isolated bacteria from the phylloplane of Hedera helix (common ivy), a widespread evergreen, using five different growth media. We further included a comparison with the uncultured phylloplane, which we show to contain the highest intra-sample diversity. Inter-sample bacterial diversity shifts from growth media most rich in nutrients to those which are more selective. The four major phyla Proteobacteria, Actinobacteria, Bacteroidetes and Firmicutes comprised the vast majority of phyla in the uncultured H. helix phylloplane, which furthermore were fully represented within growth medium samples. The plant growth promotion (PGP) profile we obtained by testing 200 isolates can help to select candidates with advantageous traits within various microbe-assisted approaches. Our isolation effort also resulted in a significant collection of bacterial strains underrepresented in public databases, mostly from the phylum Actinobacteria. ConclusionsThis study contributes as a case study of bacterial culturability and its relation with functional characteristics such as PGP potential which also is an important step towards understanding the ecological and functional role of microbial members living in the H. helix phylloplane.
Identification and Screening of Host Proteins Interacting with ORFV-ORF047&nbsp;Protein
Authors: Chen Guohua , He Xiaobing , jia Huaijie , Fang Yongxiang , Wang Xiaoxia , Lou Zhongzi , Li Weike , Jing zhizhong 
Published: August 2020

Abstract
Bankground：Orf virus (ORFV) is a member of the genus parapoxvirus and family poxviridae. The virus has a worldwide distribution and infects sheep, goats, humans, and wild animals. However, due to the complex structure of the poxvirus, the underlying mechanism of the entry and infection by ORFV remains largely unknown. ORFV ORF047 encodes a protein named L1R. Poxviral L1R serves as the receptor-binding protein and blocks virus binding and entry independently of glycosaminoglycans (GAGs). The study aimed to identify the host interaction partners of ORFV ORF047.Methods: Yeast two-hybrid cDNA library of sheep testicular cells was applied to screen the host targets with ORF047 as the bait. ORF047 was cloned into a pBT3-N vector and expressed in the NMY51 yeast strain. Then, the expression of bait proteins was validated by Western blot analysis.Results: Sheep SERP1and PABPC4 were identified as host target proteins of ORFV ORF047, and a Co-IP assay further verified their interaction. Conclusions: New host cell proteins SERP1and PABPC4 were found to interact with ORFV ORF047 and might involve in the viral mRNA translation and replication.
Evidence for Implementation of Interventions to Promote Mental Health in the Workplace: A Systematic Scoping Review Protocol
Authors: Paterson Charlotte , Leduc Caleb , Maxwell Margaret , Aust Birgit , Amann Benedikt L. , Cerga-Pashoja Arlinda , Coppens Evelien , Couwenbergh Chrisje , O’Connor Cliodhna , Arensman Ella , Greiner Birgit A. 
Published: August 2020

Abstract
Background: Mental health problems are common in the working population and represent a growing concern internationally, with potential impacts on workers, organisations, workplace health and compensation authorities, labour markets and social policies. Workplace interventions that create mental health-supportive workplaces, promote mental health awareness, destigmatise mental illness and support those with mental disorders are likely to improve health and economical outcomes for employees and organisations. Identifying factors associated with successful implementation of these interventions can improve their quality and evaluation and facilitate the uptake and expansion. Therefore, we aim to review research reporting on the implementation of mental health promotion interventions delivered in workplace settings, in order to increase understanding of factors influencing successful delivery. Methods and analysis: A scoping review will be conducted incorporating a stepwise methodology to identify relevant literature reviews, primary research and grey literature. This review is registered with Review Registry (reviewregistry897). One reviewer will conduct the search to identify English language studies in the following electronic databases from 2008 through to July 1, 2020: Scopus, PROSPERO, Health Technology Assessments, PubMed, Campbell Collaboration, Joanna Briggs Library, PsycINFO, Web of Science Core Collection, CINAHL, Institute of Occupational Safety and Health (IOSH). Reference searching, Google Scholar, Grey Matters, IOSH and expert contacts will be used to identify grey literature. Two reviewers will screen title and abstracts, aiming for 95% agreement, and then independently screen full texts for inclusion. Two reviewers will assess methodological quality of included studies using the Mixed Methods Appraisal Tool, and extract and synthesize data in line with the RE-AIM framework, Nielson and Randall’s model of organisational-level interventions and Moore’s sustainability criteria, if the data allows. We will recruit and consult with international experts in the field to ensure engagement, reach and relevance of the main findings. Discussion: This will be the first systematic scoping review to identify and synthesise evidence of barriers and facilitators to implementing mental health promotion interventions in workplace settings. Our results will inform future evaluation studies and randomised controlled trials and highlight gaps in the evidence base. Registration: reviewregistry897
The Effect of Acupuncture&nbsp;for ATP Activity in Brain Diseases: Protocol of a Systematic Review and Meta-Analysis of Animal Experiments
Authors: Yang Luping , Xiao Xili , Jiang Yijing , Zheng Yaling , Shi Lihong , Zhang Huiling , Zhong Dongling , Li Yuxi , Li Juan , Guan Li , Jin Rongjiang 
Published: August 2020

Abstract
Background: Brain is an energy-consuming organ, which is highly depended on adenosine triphosphate (ATP) supplying energy to maintain the functions of memory, thinking, cognition etc. Some animal experiment findings have indicated that ATP depletion was correlated with multiple brain diseases [brain injury, Parkinson disease (PD), stroke, brain tumor, Alzheimer’s disease (AD), vascular dementia (VD), etc.]. Acupuncture has been identified that it has a therapeutic effect for brain diseases. However, the mechanism of acupuncture for brain diseases remains to be determined. Lack of high-quality evidence of underlying mechanism could result in a limitation of acupuncture in clinical practice. Therefore, we aim to conduct a systematic review (SR) and meta-analysis of animal experiments to identify the effect of acupuncture for ATP activity in brain diseases and hope to provide a robust evidence for acupuncture in brain disease. Methods: Six databases [PubMed, Embase, web of Science, the China National Knowledge Infrastructure (CNKI), the Chinese Science and Technology Periodical Database (VIP) and Wan Fang database] will be systematically searched from the inception dates to July, 2020. Two researchers will independently screen titles and abstracts for eligibility. Eligible articles will be restricted to animal studies correlated with acupuncture treatment for brain diseases. The primary outcomes are ATP activity, ATP content, Na+-k+-ATPase activity, and Ca2+ activity. Secondary outcomes are as following: the context of glucose (Glu) and mitochondrial respiratory enzyme activity including complex I, complex II, complex III, complex V and cytochrome c (Cyt c) enzyme activity etc. Data extraction and evaluation of risk of bias will be operated by two researchers independently. The SYstematic Review Centre for Laboratory animal Experimentation (SYRCLE) tool will be utilized to examine the risk of bias. Review Manager 5.3 and Stata.12 software will be used for data synthesis.Discussion: Numerous animal experiments have been conducted and investigated the underlying mechanism of acupuncture in brain diseases. We will retrieve the current animal experiments associated with the mechanism of acupuncture and perform a SR to provide a robust evidence for acupuncture improving ATP activity in brain diseases.Systematic review registration: The registration number is CRD 42020184971.
Noise2Atom: Unsupervised Denoising for Scanning Transmission Electron Microscopy Images
Authors: Wang Feng , Henninen Trond R , Keller Debora , Erni Rolf 
Published: August 2020

Abstract
We propose an effective deep learning model to denoise scanning transmission electron microscopy (STEM) image series, named Noise2Atom, to map images from a source domain S to a target domain C, where S is for our noisy experimental dataset, and C is for the desired clear atomic images. Noise2Atom uses two external networks to apply additional constraints from the domain knowledge. This model requires no signal prior, no noise model estimation, and no paired training images. The only assumption is that the inputs are acquired with identical experimental configurations. To evaluate the restoration performance of our model, as it is impossible to obtain ground truth for our experimental dataset, we propose consecutive structural similarity (CSS) for image quality assessment, based on the fact that the structures remain much the same as the previous frame(s) within small scan intervals. We demonstrate the superiority of our model by providing evaluation in terms of CSS and visual quality on different experimental datasets.
Perceptions&nbsp;and Behavioral Adoptions&nbsp;of the Preventive Strategies from Person-to-Person Transmission of COVID-19 among the Public in China: An Online Cross-Sectional Survey
Authors: Zhu Xian , Gan Yong , Wu Bo , Gou Qinyong , Zheng Daikun , Liu Chenglu , Xu Chang , Tao Zhou , Xu Hong , Long Qian 
Published: August 2020

Abstract
Background: The strategies adopted to prevent spreading of COVID-19 are quarantine, social distancing and isolation of infected cases. This study investigates perceptions and behavioral adoptions of COVID-19 prevention strategies among the Chinese public and identified factors predicting individual health behavior.Methods: We conducted a cross-sectional online survey between 22 February and 5 March, 2020. We approached to urban residents aged over 18 years through snowball sampling method using the Chinese social media. The Health Belief Model was adopted to guide the analysis. Bivariate and multivariate logistic regressions were used to examine impacts of modifying factors (including demographic and socio-economic characteristics) and individual beliefs on individual health behavior.Results: of 5675 valid questionnaires, 95.8% of the respondents well understood the preventive measures from COVID-19 transmission, while 79.9% of the respondents adopted the behavior advised. 45.7% of the respondents perceived severity of the disease, 75.6% of the respondents perceived benefits of social constraints measures and 62.7% reported anxiety during the epidemic. After adjusting for modifying factors and individual beliefs, those who were female, had better income and good knowledge on preventive measures, perceived benefits on social constraint measures and did not feel anxiety were more likely to adopt behaviors advised.Conclusions: The Chinese public highly accepted and adopted behaviors advised to slow down the COVID-19 epidemic. People with low income or feeling anxiety were less likely to adopt the behavior advised. The policy support should target on the social vulnerable groups. The psychological support should be disseminated through different means, and the consultation should be provided to those who are in need.