bluuebunny/crossref_metadata_2025_split huggingface dataset:
Spatiotemporal Variability in Transport and Reactive Processes Across a First‐ to Fifth‐Order Fluvial Network
Authors: Gootman Kaylyn S. , González‐Pinzón Ricardo , Knapp Julia L. A. , Garayburu‐Caruso Vanessa , Cable Jaye E. 
Published: April 2020

AbstractFluvial networks integrate, transform, and transport constituents from terrestrial and aquatic ecosystems. To date, most research on water quality dynamics has focused on process understanding at individual streams, and, as a result, there is a lack of studies analyzing how physical and biogeochemical drivers scale across fluvial networks. We performed tracer tests in five stream orders of the Jemez River continuum in New Mexico, USA, to quantify reach‐scale hyporheic exchange during two different seasonal periods to address the following: How do hyporheic zone contributions to overall riverine processing change with space and time? And does the spatiotemporal variability of hyporheic exchange scale across fluvial networks? Combining conservative (i.e., bromide) and reactive (i.e., resazurin) tracer analyses with solute transport modeling, we found a dominance of reaction‐limited transport conditions and a decrease of the contributions of hyporheic processing across stream orders and flow regimes. Our field‐based findings suggest that achieving knowledge transferability of hyporheic processing within fluvial networks may be possible, especially when process variability is sampled across multiple stream orders and flow regimes. Therefore, we propose a shift in our traditional approach to investigating scaling patterns in transport processes, which currently relies on the interpretation of studies conducted in multiple sites (mainly in headwater streams) that are located in different fluvial networks, to a more cohesive, network‐centered investigation of processes using the same or readily comparable methods.
The Sensitivity of Hyporheic Exchange to Fractal Properties of Riverbeds
Authors: Lee Anzy , Aubeneau Antoine F. , Cardenas M. Bayani 
Published: April 2020

AbstractHyporheic exchange in riverbeds is driven by current‐bed topography interactions. Because riverbeds exhibit topographic roughness across scales, from individual grains to bedforms and bars, they can exhibit fractal patterns. This study analyzed the influence of fractal properties of riverbed topography on hyporheic exchange. A set of synthetic fractal riverbeds with different scaling statistics was used as inputs to sequentially coupled numerical simulations of turbulent channel flow and hyporheic flow. In the analysis, the maximum power spectrum (dune size) and the fractal dimension (topographic complexity) were considered as independent variables and we then investigated how interfacial fluxes and hyporheic travel times are functionally related to these variables. As the maximum power spectrum increases (i.e., dune height to flow depth ratio), the average interfacial flux increases logarithmically whereas it increases exponentially with an increase in fractal dimension. Hyporheic exchange is more sensitive to additional roughness (larger fractal dimensions) than to bedform size (larger maximum power). Our results imply that fractal properties of riverbeds are crucial to predicting hyporheic exchange. The predictive relationships we propose could be integrated with reduced complexity, large‐scale models. They can also be used to design artificial topographies that target hyporheic ecosystem services.
FBXO22 degrades nuclear PTEN to promote tumorigenesis
Authors: Ge Meng-Kai , Zhang Na , Xia Li , Zhang Cheng , Dong Shuang-Shu , Li Zhan-Ming , Ji Yan , Zheng Min-Hua , Sun Jing , Chen Guo-Qiang , Shen Shao-Ming 
Published: April 2020

AbstractNuclear localization of PTEN is essential for its tumor suppressive role, and loss of nuclear PTEN is more prominent than cytoplasmic PTEN in many kinds of cancers. However, nuclear PTEN-specific regulatory mechanisms were rarely reported. Based on the finding that nuclear PTEN is more unstable than cytoplasmic PTEN, here we identify that F-box only protein 22 (FBXO22) induces ubiquitylation of nuclear but not cytoplasmic PTEN at lysine 221, which is responsible for the degradation of nuclear PTEN. FBXO22 plays a tumor-promoting role by ubiquitylating and degrading nuclear PTEN. In accordance, FBXO22 is overexpressed in various cancer types, and contributes to nuclear PTEN downregulation in colorectal cancer tissues. Cumulatively, our study reports the mechanism to specifically regulate the stability of nuclear PTEN, which would provide the opportunity for developing therapeutic strategies aiming to achieve complete reactivation of PTEN as a tumor suppressor.
Microbial Diversity and Metabolic Potential in the Stratified Sansha Yongle Blue Hole in the South China Sea
Authors: He Peiqing , Xie Linping , Zhang Xuelei , Li Jiang , Lin Xuezheng , Pu Xinming , Yuan Chao , Tian Ziwen , Li Jie 
Published: April 2020

AbstractThe Sansha Yongle Blue Hole is the world’s deepest (301 m) underwater cave and has a sharp redox gradient, with oligotrophic, anoxic, and sulfidic bottom seawater. In order to discover the microbial communities and their special biogeochemical pathways in the blue hole, we analyzed the 16S ribosomal RNA amplicons and metagenomes of microbials from seawater depths with prominent physical, chemical, and biological features. Redundancy analysis showed that dissolved oxygen was the most important factor affecting the microbial assemblages of the blue hole and surrounding open sea waters, and significantly explained 44.7% of the total variation, followed by silicate, temperature, sulfide, ammonium, methane, nitrous oxide, nitrate, dissolved organic carbon, salinity, particulate organic carbon, and chlorophyll a. We identified a bloom of Alteromonas (34.9%) at the primary nitrite maximum occurring in close proximity to the chlorophyll a peak in the blue hole. Genomic potential for nitrate reduction of Alteromonas might contribute to this maximum under oxygen decrease. Genes that would allow for aerobic ammonium oxidation, complete denitrification, and sulfur-oxidization were enriched at nitrate/nitrite-sulfide transition zone (90 and 100 m) of the blue hole, but not anammox pathways. Moreover, γ-Proteobacterial clade SUP05, ε-Proteobacterial genera Sulfurimonas and Arcobacter, and Chlorobi harbored genes for sulfur-driven denitrification process that mediated nitrogen loss and sulfide removal. In the anoxic bottom seawater (100-300 m), high levels of sulfate reducers and dissimilatory sulfite reductase gene (dsrA) potentially created a sulfidic zone of ~200 m thickness. Our findings suggest that in the oligotrophic Sansha Yongle Blue Hole, O2 deficiency promotes nitrogen- and sulfur-cycling processes mediated by metabolically versatile microbials.
Alleviation of migraine symptoms by application of repetitive peripheral magnetic stimulation to myofascial trigger points of neck and shoulder muscles – A randomized trial
Authors: Renner Tabea , Sollmann Nico , Heinen Florian , Albers Lucia , Trepte-Freisleder Florian , Klose Birgit , König Helene , Krieg Sandro M. , Bonfert Michaela V. , Landgraf Mirjam N. 
Published: April 2020

AbstractMigraine is a burdensome disease with an especially high prevalence in women between the age of 15 and 49 years. Non-pharmacological, non-invasive therapeutic methods to control symptoms are increasingly in demand to complement a multimodal intervention approach in migraine. Thirty-seven subjects (age: 25.0 ± 4.1 years; 36 females) diagnosed with high-frequency episodic migraine who presented at least one active myofascial trigger point (mTrP) in the trapezius muscles and at least one latent mTrP in the deltoid muscles bilaterally prospectively underwent six sessions of repetitive peripheral magnetic stimulation (rPMS) over two weeks. Patients were randomly assigned to receive rPMS applied to the mTrPs of the trapezius (n = 19) or deltoid muscles (n = 18). Whereas the trapezius muscle is supposed to be part of the trigemino-cervical complex (TCC) and, thus, involved in the pathophysiology of migraine, the deltoid muscle was not expected to interfere with the TCC and was therefore chosen as a control stimulation site. The headache calendar of the German Migraine and Headache Society (DMKG) as well as the Migraine Disability Assessment (MIDAS) questionnaire were used to evaluate stimulation-related effects. Frequency of headache days decreased significantly in both the trapezius and the deltoid group after six sessions of rPMS (trapezius group: p = 0.005; deltoid group: p = 0.003). The MIDAS score decreased significantly from 29 to 13 points (p = 0.0004) in the trapezius and from 31 to 15 points (p = 0.002) in the deltoid group. Thus, rPMS applied to mTrPs of neck and shoulder muscles offers a promising approach to alleviate headache frequency and symptom burden. Future clinical trials are needed to examine more profoundly these effects, preferably using a sham-controlled setting.
Synaptic ribbons foster active zone stability and illumination-dependent active zone enrichment of RIM2 and Cav1.4 in photoreceptor synapses
Authors: Dembla Ekta , Dembla Mayur , Maxeiner Stephan , Schmitz Frank 
Published: April 2020

AbstractRod photoreceptor synapses use large, ribbon-type active zones for continuous synaptic transmission during light and dark. Since ribbons are physically connected to the active zones, we asked whether illumination-dependent changes of ribbons influence Cav1.4/RIM2 protein clusters at the active zone and whether these illumination-dependent effects at the active zone require the presence of the synaptic ribbon. We found that synaptic ribbon length and the length of presynaptic Cav1.4/RIM2 clusters are tightly correlated. Dark-adaptation did not change the number of ribbons and active zone puncta. However, mean ribbon length and length of presynaptic Cav1.4/RIM2 clusters increased significantly during dark-adaptation when tonic exocytosis is highest. In the present study, we identified by the analyses of synaptic ribbon-deficient RIBEYE knockout mice that synaptic ribbons are (1) needed to stabilize Cav1.4/RIM2 at rod photoreceptor active zones and (2) are required for the darkness-induced active zone enrichment of Cav1.4/RIM2. These data propose a role of the ribbon in active zone stabilization and suggest a homeostatic function of the ribbon in illumination-dependent active zone remodeling.
Non-Invasive Photoacoustic Imaging of In Vivo Mice with Erythrocyte Derived Optical Nanoparticles to Detect CAD/MI
Authors: Liu Yonggang , Hanley Taylor , Chen Hao , Long Steven R. , Gambhir Sanjiv S. , Cheng Zhen , Wu Joseph C. , Fakhri Georges El , Anvari Bahman , Zaman Raiyan T. 
Published: April 2020

AbstractCoronary artery disease (CAD) causes mortality and morbidity worldwide. We used near-infrared erythrocyte-derived transducers (NETs), a contrast agent, in combination with a photoacoustic imaging system to identify the locations of atherosclerotic lesions and occlusion due to myocardial-infarction (MI). NETs (≈90 nm diameter) were fabricated from hemoglobin-depleted mice erythrocyte-ghosts and doped with Indocyanine Green (ICG). Ten weeks old male C57BL/6 mice (n = 9) underwent left anterior descending (LAD) coronary artery ligation to mimic vulnerable atherosclerotic plaques and their rupture leading to MI. 150 µL of NETs (20 µM ICG,) was IV injected via tail vein 1-hour prior to photoacoustic (PA) and fluorescence in vivo imaging by exciting NETs at 800 nm and 650 nm, respectively. These results were verified with histochemical analysis. We observed ≈256-fold higher PA signal from the accumulated NETs in the coronary artery above the ligation. Fluorescence signals were detected in LAD coronary, thymus, and liver. Similar signals were observed when the chest was cut open. Atherosclerotic lesions exhibited inflammatory cells. Liver demonstrated normal portal tract, with no parenchymal necrosis, inflammation, fibrosis, or other pathologic changes, suggesting biocompatibility of NETs. Non-invasively detecting atherosclerotic plaques and stenosis using NETs may lay a groundwork for future clinical detection and improving CAD risk assessment.
Achieving affective human–virtual agent communication by enabling virtual agents to imitate positive expressions
Authors: Numata Takashi , Sato Hiroki , Asa Yasuhiro , Koike Takahiko , Miyata Kohei , Nakagawa Eri , Sumiya Motofumi , Sadato Norihiro 
Published: April 2020

AbstractAffective communication, communicating with emotion, during face-to-face communication is critical for social interaction. Advances in artificial intelligence have made it essential to develop affective human–virtual agent communication. A person’s belief during human–virtual agent interaction that the agent is a computer program affects social-cognitive processes. Whether this belief interferes with affective communication is an open question. We hypothesized that the imitation of a positive emotional expression by a virtual agent induces a positive emotion, regardless of the belief. To test this hypothesis, we conducted an fMRI study with 39 healthy volunteers, who were made to believe that a virtual agent was either a person or a computer. They were instructed to smile, and immediately afterwards, the virtual agent displayed a positive, negative, or neutral expression. The participants reported a positive emotion only when their smile was imitated by the agent’s positive expression regardless of their belief. This imitation activated the participants’ medial prefrontal cortex and precuneus, which are involved in anthropomorphism and contingency, respectively. These results suggest that a positive congruent response by a virtual agent can overcome the effect of believing that the agent is a computer program and thus contribute to achieving affective human–virtual agent communication.
Effect of step frequency on leg stiffness during running in unilateral transfemoral amputees
Authors: Hobara Hiroaki , Sakata Hiroyuki , Namiki Yuta , Hisano Genki , Hashizume Satoru , Usui Fumio 
Published: April 2020

AbstractSpring-like leg behavior is a general feature of mammalian bouncing gaits, such as running and hopping. Although increases in step frequency at a given running speed are known to increase the stiffness of the leg spring (kleg) in non-amputees, little is known about stiffness regulation in unilateral transfemoral amputees. In this study, we investigated stiffness regulation at different step frequencies at a given running speed in unilateral transfemoral amputees. We recruited nine unilateral transfemoral amputees wearing running-specific prostheses. They were asked to perform the action of running across a range of step frequencies (±20, ±15, ±10, ±5, and 0% of their preferred step frequency) at a given speed on an instrumented treadmill. The kleg values were calculated using ground reaction force data in both the affected and unaffected limbs. It was found that kleg increased with increasing step frequency for the unaffected limb, but not for the affected limb. Consequently, the unilateral transfemoral amputees attained the desired step frequency in the unaffected limb, but were unable to match the three highest step frequencies using their affected limbs. These results suggest that the stiffness regulation strategy during running differs between the affected and unaffected limbs.
Variable protein expression in marine-derived filamentous fungus Penicillium chrysogenum in response to varying copper concentrations and salinity
Authors: Lotlikar Nikita , Damare Samir , Meena Ram Murti , Jayachandran Saranya 
Published: April 2020

Abstract
Copper is one of the essential trace dietary minerals for all living organisms, but is potentially toxic at higher concentrations, mainly due to the redox reactions in its transition state. Tolerance of microbes towards copper is primarily attributed to chelation and biosorption. In this study, marine-derived filamentous fungi were evaluated for their ability to remove Cu(ii) from a culture medium. Further, the cellular response of a select isolate to salinity stress (0, 35 and 100 PSU) and Cu(ii) stress (0, 100, and 500 ppm) was studied using the peptide mass fingerprinting technique, which revealed expression of 919 proteins, of which 55 proteins were commonly expressed across all conditions. Housekeeping proteins such as citrate synthase, pyruvate carboxylase, ribosomal proteins, ATP synthases, and more were expressed across all conditions. Reactive oxygen species scavenging proteins such as glutaredoxin, mitochondrial peroxiredoxins and thioredoxins were expressed under Cu(ii) and salinity stresses individually as well as in combination. Up-regulation of glutaredoxin under Cu(ii) stress with fold change values of 18.3 and 13.9 under 100 ppm and 500 ppm of Cu(ii) indicated active scavenging of free radicals to combat oxidative damage. The common mechanisms reported were enzymatic scavenging of free radicals, activation of DNA damage and repair proteins and probable intracellular metal chelation. This indicated multiple stress mechanisms employed by the isolate to combat the singular and synergistic effects of Cu(ii) and salinity stress.
A study of the reactions of Ni+and NiO+ions relevant to planetary upper atmospheres
Authors: Bones David L. , Daly Shane M. , Mangan Thomas P. , Plane John M. C. 
Published: April 2020

The reactions between Ni+ and O3, O2, N2, CO2 and H2O; NiO+ and O, O3 and CO; and NiO2+ and O3 were studied at 294 K using the pulsed laser ablation of a nickel metal target in a fast flow tube, with mass spectrometric detection of Ni+ and NiO+.
LncRNA LINC00668 promotes cell proliferation, migration, invasion ability and EMT process in hepatocellular carcinoma by targeting miR-532-5p/YY1 axis
Authors: Xuan Wei , Zhou Chen , You Guangqiang 
Published: April 2020

AbstractLiver cancer is now one of the most lethal and commonest cancers in the world, among which over 90% is hepatocellular carcinoma (HCC). Recent studies have confirmed long non-coding RNAs (lncRNAs) are implicated in carcinogenesis. It has been reported lncRNA LINC00668 serves as an oncogene in several cancers. However, the mechanism where LINC00668 regulates HCC is still unclear. qRT-PCR analysis was adopted to detect the expression of relative RNAs. Cytoplasmic and nuclear RNA fraction analysis was conducted to verify the underlying molecular mechanism. Cell colony formation was carried out to test cell colony formation ability and transwell assays were performed to testify cell migratory and invaded abilities. Relevant protein expression level was measured by Western blot assay. LINC00668 was significantly up-regulated in HCC tissues and cell lines. LINC00668 knockdown inhibited cell proliferative, migratory and invasion abilities and slowed down the epithelial–mesenchymal transition (EMT) process. Mechanistically, LINC00668 positively modulates the expression of YY1 by competitively binding to miR-532-5p. It was revealed that LINC00668 up-regulation accelerated cell proliferation and motility in HCC and suggested LINC00668 could be a potential therapeutic target for HCC.
Structure of the outer Galactic disc with Gaia DR2
Authors: Chrobáková Ž. , Nagy R. , López-Corredoira M. 
Published: April 2020

Context. The structure of outer disc of our Galaxy is still not well described, and many features need to be better understood. The second Gaia data release (DR2) provides data in unprecedented quality that can be analysed to shed some light on the outermost parts of the Milky Way.
Aims. We calculate the stellar density using star counts obtained from Gaia DR2 up to a Galactocentric distance R = 20 kpc with a deconvolution technique for the parallax errors. Then we analyse the density in order to study the structure of the outer Galactic disc, mainly the warp.
Methods. In order to carry out the deconvolution, we used the Lucy inversion technique for recovering the corrected star counts. We also used the Gaia luminosity function of stars with MG <  10 to extract the stellar density from the star counts.
Results. The stellar density maps can be fitted by an exponential disc in the radial direction hr = 2.07 ± 0.07 kpc, with a weak dependence on the azimuth, extended up to 20 kpc without any cut-off. The flare and warp are clearly visible. The best fit of a symmetrical S-shaped warp gives zw ≈ z⊙ + (37 ± 4.2(stat.) − 0.91(syst.))pc ⋅ (R/R⊙)2.42 ± 0.76(stat.) + 0.129(syst.)sin(ϕ + 9.3° ±7.37° (stat.) + 4.48° (syst.)) for the whole population. When we analyse the northern and southern warps separately, we obtain an asymmetry of an ∼25% larger amplitude in the north. This result may be influenced by extinction because the GaiaG band is quite prone to extinction biases. However, we tested the accuracy of the extinction map we used, which shows that the extinction is determined very well in the outer disc. Nevertheless, we recall that we do not know the full extinction error, and neither do we know the systematic error of the map, which may influence the final result. The analysis was also carried out for very luminous stars alone (MG <  −2), which on average represents a younger population. We obtain similar scale-length values, while the maximum amplitude of the warp is 20 − 30% larger than with the whole population. The north-south asymmetry is maintained.
Dynamische OCT − was verraten uns die Gefäße?
Authors: Schuh S. , Berger M. , Brunnmeier G. , Welzel J. 
Published: April 2020

ZusammenfassungDie Weiterentwicklung der optischen Kohärenztomografie (OCT) zur dynamischen OCT (D-OCT) erlaubt die gleichzeitige Darstellung der oberflächlichen Blutgefäße. Blut ist das wichtigste Transportsystem unseres Körpers, und sowohl durch äußere als auch durch innere Einflüsse können physiologische Prozesse beeinträchtigt werden. Die D-OCT ermöglicht erstmals die Darstellung dieser Veränderungen anhand der Gefäßdichte und Durchblutung. Durch wiederholtes schnelles Messen der gleichen untersuchten Region können mithilfe einer speziellen Software Unterschiede in einer Bildserie erfasst werden und so sich bewegende Teilchen, die den Blutbestandteilen entsprechen, bis zu einer Tiefe von 0,5 mm vom statischen Hautgewebe differenziert werden. Die Software bildet den Blutfluss im D-OCT-Bild in roter Farbe als Überlagerung über das grau-weiß strukturelle Gewebe ab. Mithilfe der D-OCT konnten physiologische Einflüsse auf die Durchblutung, wie Vasokonstriktion und Positionsänderung, Unterschiede aufgrund der anatomischen Körperregionen, aber auch therapeutische Effekte, wie z. B. durch die lokale Behandlung mit vasokonstringierendem Brimonidin-Gel (einem selektiven α2-Adrenorezeptor-Agonisten) oder bei der Lasertherapie nachgewiesen werden. Weiterhin fanden sich bestimmte Blutgefäßformen nur bei chronisch venösen Ulzera (wie Knäuel am Wundrand oder geschlängelte Gefäße bei Dermatosklerose), während die Blutgefäße bei akuten Wunden eine Häufung von anderen Gefäßformen und Verteilungsmustern zeigten. Bei ausgewählten entzündlichen und autoimmunbedingten Erkrankungen konnten die damit einhergehenden vaskulären Prozesse exemplarisch dargestellt werden. Auch ließ sich mit der D-OCT feststellen, dass eine erhöhte Blutgefäßdichte und gewisse Formen erst mit einer größeren Tumordicke von Melanomen (Breslow-Index) einhergehen. Außerdem können die Blutgefäße zur Differenzierung sowie zur Therapieauswahl von nicht-melanozytärem Hautkrebs beitragen. Die D-OCT ist jedoch nicht nur ein diagnostisches Gerät, sondern eignet sich auch zur bildgeführten Therapie wie der D-OCT-geführten Lasertherapie.Die D-OCT bietet durch die Darstellung von Blutgefäßen zahlreiche diagnostische und therapeutische Einsatzmöglichkeiten. Die Blutgefäße verraten uns so viel über physiologische und pathologische Prozesse in unserer Haut, und ihre Darstellung wird in Zukunft eine individuellere, patienten-orientiertere Therapie erlauben, als es bisher möglich ist. So könnten z. B. Ulzera verschiedener Genese mittels D-OCT differenziert werden oder Melanome anhand der Gefäßmuster bereits präoperativ risikostratifiziert werden.