bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of serelaxin in patients admitted for acute heart failure: a meta‐analysis
Authors: Teerlink John R. , Davison Beth A. , Cotter Gad , Maggioni Aldo P. , Sato Naoki , Chioncel Ovidiu , Ertl Georg , Felker G. Michael , Filippatos Gerasimos , Greenberg Barry H. , Pang Peter S. , Ponikowski Piotr , Edwards Christopher , Senger Stefanie , Teichman Sam L. , Nielsen Olav Wendelboe , Voors Adriaan A. , Metra Marco 
Published: December 2019

AbstractAimsThe effectiveness and safety of 48 h intravenous 30 μg/kg/day serelaxin infusion in acute heart failure (AHF) has been studied in six randomized, controlled clinical trials.Methods and resultsWe conducted a fixed‐effect meta‐analysis including all studies of intravenous serelaxin initiated within the first 16 h of admission for AHF. Endpoints considered were the primary and secondary endpoints examined in the serelaxin phase III studies. In six randomized controlled trials, 6105 total patients were randomized to receive intravenous serelaxin 30 μg/kg/day and 5254 patients to control. Worsening heart failure to day 5 occurred in 6.0% and 8.1% of patients randomized to serelaxin and control, respectively (hazard ratio 0.77, 95% confidence interval 0.67–0.89; P = 0.0002). Serelaxin had no statistically significant effect on length of stay, or cardiovascular death, or heart or renal failure rehospitalization. Serelaxin administration resulted in statistically significant improvement in markers of renal function and reductions in both N‐terminal pro‐B‐type natriuretic peptide and troponin. No significant adverse outcomes were noted with serelaxin. Through the last follow‐up, which occurred at an average of 4.5 months (1–6 months), serelaxin administration was associated with a reduction in all‐cause mortality, with an estimated hazard ratio of 0.87 (95% confidence interval 0.77–0.98; P = 0.0261).ConclusionsAdministration of intravenous serelaxin to patients admitted for AHF was associated with a highly significant reduction in the risk of 5‐day worsening heart failure and in changes in renal function markers, but not length of stay, or cardiovascular death, or heart or renal failure rehospitalization. Serelaxin administration was safe and associated with a significant reduction in all‐cause mortality.
Salt‐effect enhanced hollow‐fiber liquid‐phase microextraction of glutathione in human saliva followed by miniaturized capillary electrophoresis with amperometric detection
Authors: Luo Dan , Fang Zhonghui , Zhao Xiaoshuang , Ma Yaolu , Ye Jiannong , Chu Qingcui 
Published: December 2019

AbstractA hollow‐fiber liquid‐phase microextraction (HF‐LPME) method was established for purification and enrichment of glutathione (GSH) in human saliva followed by a miniaturized capillary electrophoresis with amperometric detection system (mini‐CE‐AD). Based on regulating isoelectric point and increasing salt effect to modify donor phase, HF‐LPME could provide high enrichment efficiency for GSH up to 471 times, and the extract was directly injected for mini‐CE‐AD analysis. The salt‐effect enhanced HF‐LPME/mini‐CE‐AD method has been successfully applied to saliva analysis, and acceptable LOD (0.46 ng/mL, S/N = 3) and recoveries (92.7–101.3%) could be obtained in saliva matrix. The sample pretreatment of this developed method was simple and required no derivatization, providing a potential alternative for non‐invasive fluid analysis using portable instrument.
Dynamic response and impact energy loss in controlled rocking members
Authors: Kalliontzis Dimitrios , Sritharan Sri 
Published: December 2019

SummaryUnbonded posttensioning anchors a rocking structural member to its foundation and produces its controlled rocking response when the member undergoes seismic action. Unlike rocking of free‐standing bodies, little attention has been given to the dynamic behavior of these controlled rocking members. This paper utilizes experiments of concrete structural members with unbonded posttensioning, varying member geometries, and levels of initial posttensioning force to (a) characterize the associated impact energy loss and (b) improve modeling of controlled rocking motions. Experimental results show that impact energy loss in controlled rocking members can be captured accurately using the coefficient of restitution (r) approach of the modified simple rocking model (MSRM). Based on the MSRM, a controlled rocking model (CRM) is developed that additionally accounts for the variations in contact length at the member‐to‐foundation (rocking) interface. The CRM reproduces the experimental responses of controlled rocking members with good accuracy and is used to investigate controlled rocking motions under horizontal base excitations.
Antibodies raised against a Sunn bug (Eurygaster integriceps Put.) recombinant protease, rGHP3p2, can inhibit gluten‐hydrolyzing activity
Authors: Dolgikh Vyacheslav , Tsarev Alexander , Timofeev Sergey , Zhuravlyov Vladimir , Senderskiy Igor , Lovegrove Alison , Konarev Alexander 
Published: December 2019

AbstractSunn pest or Sunn bug, Eurygaster integriceps Put., salivary gland proteases are responsible for the deterioration of wheat flour quality during dough mixing, resulting from gluten hydrolysis. These proteases are highly heterogeneous and show low sensitivity to most types of proteinaceous inhibitors, meaning that such inhibitors cannot be used to prevent gluten damage. The present study describes the generation of a specific peptide antibody, raised against the active center of the recombinant gluten‐hydrolyzing protease (GHP3). The recombinant protein, encoding two repeats of the GHP3 sequence element involved in forming the S4 pocket and binding of substrate at position P4, was designed and expressed in Escherichia coli. The antibodies raised to this recombinant protein showed inhibitory activity against the GHP3 protease. The results indicate that it is possible to design specific antibodies to inhibit wheat‐bug gluten‐hydrolyzing proteases.
Conservative treatment of asymptomatic ectopic ureterocele: A report of two cases
Authors: Maruo Kazutaka , Nishinaka Kazuyuki 
Published: December 2019

IntroductionThere are no established treatments for asymptomatic ectopic ureteroceles, with completely duplicated ureters, during the neonatal period and infancy. However, conservative treatment is sometimes successful.Case presentationTwo patients were prenatally diagnosed, via ultrasonography, with left hydronephrosis. In each case, magnetic resonance imaging confirmed an ectopic ureterocele accompanying the left, completely duplicated ureter. Prophylactic antibiotics were administered and conservative treatment was started. Currently, one patient is 44 months old and the other is 49 months old; in neither patient has the ureterocele or hydronephrosis been exacerbated.ConclusionPatients with (i) a nonfunctional kidney, (ii) mild hydronephrosis or moderate vesicoureteral reflux, (iii) no bladder neck obstruction on urination, and (iv) a Churchill classification ≤Grade II (Churchill classification) may be able to select conservative treatment.
Reframing and Repositioning College Readers’ Assumptions About Reading Through Eye Movement Miscue Analysis
Authors: Porter Heather D. , Kim Koomi , Franzak Judith K. , MacDonald Katherine 
Published: December 2019

AbstractAs one of multiple ways to explore the reading process, eye movement miscue analysis is a tool that provides a continuous record of eye fixations and movements over an entire text, and a record of the oral reading of that text and the miscues (observed responses) that readers produce. The authors present profiles of two successful college readers who doubted their reading efficacy. Using data from eye tracking, miscue analysis, and the retelling, the authors invited the readers to examine their assumptions about reading and how they positioned themselves as readers. Data presented were drawn from the readers’ eye movements during the reading of two texts—one an informational text and the other a constructed text with embedded errors—and are discussed in relation to the readers’ perceptions of reader identity and processes. Implications for teachers include strategies for helping readers address common misconceptions about reading and reclaim their role as meaning makers.
Structural characterization, mechanical, and electrochemical studies of hydroxyapatite‐titanium composite coating fabricated using electrophoretic deposition and reaction bonding process
Authors: Maleki‐Ghaleh Hossein , Khalil‐Allafi Jafar , Horandghadim Nazila , Keikhosravani Pardis , Hosseini Mir Ghasem 
Published: December 2019

AbstractIn the present work, hydroxyapatite (HA)‐titanium (Ti, 20 wt%) composite coating was coated on NiTi alloy substrate by EPD (electrophoretic deposition) process. Before applying the coating, the HA powder was composed with Ti powder using a ball milling process. Influence of the ball milling time on morphology and phase structure of HA‐Ti powder was evaluated using TEM and XRD analysis. After composing the HA particles with Ti, the HA‐Ti composite powders were coated on the NiTi substrate by the EPD process in an n‐butanol medium for 2 min, with the applied voltage of 60 V. XRD and SEM analysis were utilized to evaluate the phase analysis and morphology of the coatings. Mechanical and electrochemical characteristic of the coatings were also assessed using the micro‐indentation, micro‐scratch, and polarization tests, respectively. The results revealed that the milling process time had a significant influence on reaction bonds and optimum mixing time was 4 hr. Micro‐hardness of the HA‐Ti composite coating (304 HV) was substantially higher than the HA coating (72 HV). Also, as the HA coating was composed with Ti particles, the amount of force (in the micro‐scratch test) required for detaching the coating from the NiTi substrate increased from 7.1 to 17.8 N. The polarization results showed that the HA‐Ti composite coating had a higher electrochemical resistance compared with the HA coating. Corrosion resistance of the NiTi alloy coated with HA increased from 133 kΩ.cm2 to 2,720 kΩ.cm2 after composed with the Ti particles.
Identification of genes related to low‐grade glioma progression and prognosis based on integrated transcriptome analysis
Authors: Jiang Yao , He Jimin , Guo Yongcan , Tao Hualin , Pu Fei , Li Yiqin 
Published: December 2019

AbstractGlioma is one of the most common types of human brain tumor, with high mortality in high‐grade gliomas (HGG). Low‐grade gliomas (LGG) can progress into HGG, leading to poor prognosis. However, it is unclear what factors affect the progression of LGG to HGG. This study aims to explore the function of the crosstalk genes on the progression and prognosis of LGG using bioinformatics analysis. Integrated transcriptome analysis was used to screen differentially expressed genes (DEGs). Then, gene ontology (GO) function enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were performed to investigate the association between DEGs and gene functions and pathways by ClusterProfiler package and ClueGO plug‐in. Protein‐protein interaction (PPI) network analysis was applied to explore the connection between genes and biological processes. Subsequently, the gene clusters were analyzed using the Centiscape and molecular complex detection (MCODE) plug‐in in Cytoscape software, where the crosstalk genes were identified for further study. Ultimately, the UALCAN website and Gene Expression Profiling Interactive Analysis (GEPIA) website were performed to visualize the expression levels and survival curves of genes, respectively. There were 74 DEGs identified in glioma, including 55 upregulated genes and 19 downregulated genes, which mainly were enriched in extracellular matrix (ECM)‐receptor interaction, focal adhesion, PI3K‐Akt signaling pathway, and so on. Then, six crosstalk genes were selected, including COL1A1, COL1A2, COL3A1, COL4A1, COL4A2, and COL5A2 genes. Overall survival (OS) analysis of crosstalk genes was conducted on the GEPIA website. High expression levels of crosstalk genes were closely related to the low survival rate of patients with LGG. The overexpressed crosstalk genes, such as COL1A1, COL1A2, COL3A1, COL4A1, COL4A2, and COL5A2 may participate in the progression and poor prognosis of LGG through the ECM‐receptor interaction pathway.
Polyvinyl alcohol modified polyvinylidene fluoride‐graphene oxide scaffold promotes osteogenic differentiation potential of human induced pluripotent stem cells
Authors: Azadian Esmaeel , Arjmand Bahar , Ardeshirylajimi Abdolreza , Hosseinzadeh Simzar , Omidi Meisam , Khojasteh Arash 
Published: December 2019

AbstractTissue engineering is fast becoming a key approach in bone medicine studies. Designing the ideally desirable combination of stem cells and scaffolds are at the hurt of efforts for producing implantable bone substitutes. Clinical application of stem cells could be associated with serious limitations, and engineering scaffolds that are able to imitate the important features of extracellular matrix is a major area of challenges within the field. In this study, electrospun scaffolds of polyvinylidene fluoride (PVDF), PVDF‐graphene oxide (GO), PVDF‐polyvinyl alcohol (PVA) and PVDF‐PVA‐GO were fabricated to study the osteogenic differentiation potential of human induced pluripotent stem cells (iPSCs) while cultured on fabricated scaffolds. Scanning electron microscopy study, viability assay, relative gene expression analysis, immunocytochemistry, alkaline phosphates activity, and calcium content assays confirmed that the osteogenesis rate of hiPSCs cultured on PVDF‐PVA‐Go is significantly higher than other scaffolds. Here, we showed that the biocompatible, nontoxic, flexible, piezoelectric, highly porous and interconnected three‐dimensional structure of electrospun PVDF‐PVA‐Go scaffold in combination with hiPSCs (as the stem cells with significant advantageous in comparison to other types) makes them a highly promising scaffold‐stem cell system for bone remodeling medicine. There was no evidence for the superiority of PVDF‐GO or PVDF‐PVA scaffold for osteogenesis, compared to each other; however both of them showed better potentials as to PVDF scaffold.
FFParam: Standalone package for CHARMM additive and Drude polarizable force field parametrization of small molecules
Authors: Kumar Anmol , Yoluk Ozge , MacKerell Alexander D. 
Published: December 2019

AbstractAccurate force‐field (FF) parameters are key to reliable prediction of properties obtained from molecular modeling (MM) and molecular dynamics (MD) simulations. With ever‐widening applicability of MD simulations, robust parameters need to be generated for a wider range of chemical species. The CHARMM General Force Field program (CGenFF, https://cgenff.umaryland.edu/) is a tool for obtaining initial parameters for a given small molecule based on analogy with the available CGenFF parameters. However, improvement of these parameters is often required and performing their optimization remains tedious and time consuming. In addition, tools for optimization of small molecule parameters in the context of the Drude polarizable FF are not yet available. To overcome these issues, the FFParam package has been designed to facilitate the parametrization process. The package includes a graphical user interface (GUI) created using Qt libraries. FFParam supports Gaussian and Psi4 for performing quantum mechanical calculations and CHARMM and OpenMM for MM calculations. A Monte Carlo simulated annealing (MCSA) algorithm has been implemented for automated fitting of partial atomic charge, atomic polarizabilities and Thole scale parameters. The LSFITPAR program is called for automated fitting of bonded parameters. Accordingly, FFParam provides all the features required for generation and analysis of CHARMM and Drude FF parameters for small molecules. FFParam‐GUI includes a text editor, graph plotter, molecular visualization, and text to table converter to meet various requirements of the parametrization process. It is anticipated that FFParam will facilitate wider use of CGenFF as well as promote future use of the Drude polarizable FF.
Stress responses in the chemistry and mRNA abundance of the peripheral blood in the American alligator
Authors: Kohno Satomi , Zhu Jianguo , Guillette Louis J. 
Published: December 2019

AbstractTo monitor physiological and toxicological conditions in an endangered species, noninvasive to minimally invasive sampling methods are needed. We analyzed peripheral blood cells to determine if we could monitor some physiological responses of the American alligator following capture stress. Juvenile American alligators were restrained for 16 h to examine the stress response in plasma and blood cells. Plasma corticosterone concentrations were increased by restraint as were plasma concentrations of aspartate aminotransferase (AST), creatine kinase (CK), uric acid, and glucose; a sexually dimorphic response was seen in AST and CK concentrations. The lapse time of restraint was associated with altered messenger RNA (mRNA) levels of the glucocorticoid receptor (GCR) in red blood cells and JUN proto‐oncogene in both white and red blood cells. A two‐way cluster analysis revealed that two major clusters of factors were associated with the responses seen: (a) mRNA levels of GCR and heat‐shock proteins in both blood cells were associated with plasma corticosterone concentration, whereas (b) androgen receptors and JUN mRNA levels in both blood cells were associated with cloacal temperature and body composition. Blood cells appear to be an excellent source to examine the cellular stress response to steroid hormone signals in mRNA levels. We propose that this approach, using blood cells, could provide essential insights into the molecular responses associated with stress in reptiles as well as many other nontraditional model species, including endangered species.
Domino synthesis of pyrimido and imidazoquinazolinones
Authors: Fathalla Walid , Nofal Eman Y. , El‐Moneim Mohamed Abd 
Published: December 2019

AbstractA simple method for the synthesis of N‐alkyl‐2‐arylquinazolin‐4‐amines, methyl 4‐((2‐arylquinazolin‐4‐yl)amino) butanoates, 6‐aryl‐2,3‐dihydro‐4H‐pyrimido[1,2‐c]quinazolin‐4‐ones, and 5‐arylimidazo[1,2‐c]quinazolin‐3(2H)‐ones has been described. It involves a simple reaction of N‐(2‐cyanophenyl)‐substitutedbenzimidoyl chlorides with alkylamine, γ‐aminobutyric acid, β‐alanine, l‐alanine, and glycine methyl esters hydrochloride in acetonitrile to afford the desired compounds after a series of instantaneous reactions that include Dimroth rearrangement. The reaction involves reflux for 12 hours, simple addition of reagents to an in situ generated benzimidoyl chloride, and simple workup, to form 21 examples of pure compounds in high yields. The active intermediate N‐(2‐cyanophenyl)‐substitutedbenzimidoyl chlorides were formed by the reaction of N‐(2‐cyanophenyl)‐substitutedbenzamides with thionyl chloride in a one‐pot strategy. The alternative method described for this preparation deals with an exhausting multistep reactions starting from anthranilic acid.
Thiazolidine‐4‐one clubbed pyrazoles hybrids: Potent α‐amylase and α‐glucosidase inhibitors with NLO properties
Authors: Kumar Parvin , Duhan Meenakshi , Sindhu Jayant , Kadyan Kulbir , Saini Sangeeta , Panihar Neeraj 
Published: December 2019

AbstractMolecular hybrids based on thiazolidin‐4‐one and pyrazolyl pharmacophore (THZP) as new antidiabetic agents were synthesized. Two sets of signals came into view in 1H NMR of THZP8‐THZP14 exhibited the presence of a configurational isomeric mixture of 2E,5Z (38.24%‐41.58%) and 2Z,5Z isomers (58.42%‐61.76%), which was further endorsed by density functional theory (DFT) studies. All the compounds exhibit promising nonlinear optical properties (NLO). Further, the biological potential of THZPs was explored in terms of α‐amylase and α‐glucosidase inhibition. DFT‐based descriptors were calculated to describe the reactivity, and a relationship was developed with biological activities. THZP9 and THZP14 showed remarkable inhibition of α‐amylase and α‐glucosidase with IC50 9.90μM and 4.84μM, respectively, as compared with standard drug acarbose.
Copulatory mechanics in the wolf spider Agalenocosa pirity reveals a hidden diversity of locking systems in Lycosidae (Araneae)
Authors: Poy Dante , Ramírez Martín J. , Michalik Peter , Piacentini Luis N. 
Published: December 2019

AbstractGenital traits are among the fastest to evolve, and the processes that drive their evolution are intensively studied. Spiders are characterized by complex genitalia, but the functional role of the different structures during genital coupling is largely unknown. Members of one of the largest spider groups, the retrolateral tibial apophysis (RTA) clade, are characterized by a RTA on the male palp, which is thought to play a crucial role during genital coupling. However, the RTA was lost in several families including the species‐rich wolf spiders (Lycosidae) leading to the hypothesis that the genital coupling is achieved by alternative mechanisms. Here, we investigate the genital interactions during copulation in the wolf spider Agalenocosa pirity (Zoicinae) on cryofixed mating pairs using electron, optical and X‐ray microscopy and compare our findings with other lycosids and entelegyne spiders. We found an unprecedented coupling mechanism for lycosid spiders involving the palea and a membranous cuticle folding adjacent to the epigynal plate. Additionally, we show an uncommon coupling between the median apophysis and the contralateral genital opening, and confirmed that the terminal apophysis acts as functional conductor, as previously hypothesized for males of Zoicinae. Phylogenetic mapping of RTA indicated that the basal tibial process found in Agalenocosa is a secondary acquisition rather than a modified RTA.
Reconstructive and prosthodontic outcomes after multiple palatomaxillary reconstructions
Authors: Sharif Kayvon F. , Sims John R. , Yue Lauren E. , Baik Fred M. , Kiplagat Kimberly J. , Buchbinder Daniel , Okay Devin J. , Chai Raymond L. , Urken Mark L. 
Published: December 2019

ObjectiveA subset of patients who undergo major palatomaxillary reconstruction do not initially achieve their intended oncologic and/or reconstructive goals and require additional surgery. We aim to detail the unique management considerations in this patient population.MethodsWe performed a retrospective review of patients who underwent palatomaxillary reconstruction by the senior author (m.l.u.) between 1998 and 2016.ResultsTwenty‐one patients required multiple reconstructions. The median time to second reconstruction was 17 months. The most common reason for a second reconstruction was for recurrent disease (10 of 21), followed by functional/aesthetic reasons (7 of 21) and osteoradionecrosis (4 of 21). Four patients went on to have a third reconstruction, and two underwent a fourth. A total of 27 reconstructions were performed, consisting of 20 soft tissue free flaps, four vascularized bone free flaps, and three locoregional flaps.ConclusionThis patient cohort represents unique oncologic and reconstructive challenges. With long‐term follow‐up, multiple reconstructions may be required to optimize oncologic and functional/aesthetic outcomes. This is the first series of its kind that details the reasons for, as well as the outcomes of, patients who required multiple reconstructive procedures following initial palatomaxillary reconstruction.Level of Evidence2B Laryngoscope, 130:2349–2353, 2020