bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fidelity Assessment of a Social Work–Led Intervention Among Patients With Firearm Injuries
Authors: Lyons Vivian H. , Benson Lina R. , Griffin Elizabeth , Floyd Anthony S. , Kiche Sharon W. , Haggerty Kevin P. , Whiteside Lauren , Conover Sarah , Herman Daniel B. , Rivara Frederick P. , Rowhani-Rahbar Ali 
Published: March 2020

Purpose: To support future development and refinement of social work–led intervention programs among patients with firearm injuries and to demonstrate how a fidelity assessment can be used to adjust and refine intervention delivery in an ongoing trial. Method: We conducted a fidelity assessment of a randomized controlled trial of a social work–led intervention among patients with a firearm injury. Results: We found that our study intervention was well implemented, meeting 70% of the fidelity assessment score items, however, noted lower fidelity with client-based items. Discussion: As a result of fidelity assessment findings, we refined intervention delivery to improve implementation fidelity including beginning to review cases of all patients each month rather than focusing on patients in crisis. Our fidelity assessment process and findings offer insight into the challenges of implementing an intervention among patients with firearm injuries and highlight the value of monitoring intervention fidelity during an ongoing trial.
Utstein recommendation for emergency stroke care
Authors: Rudd AG , Bladin C , Carli P , De Silva DA , Field TS , Jauch EC , Kudenchuk P , Kurz MW , Lærdal T , Ong MEH , Panagos P , Ranta A , Rutan C , Sayre MR , Schonau L , Shin SD , Waters D , Lippert F 
Published: March 2020

Background Recent advances in treatment for stroke give new possibilities for optimizing outcomes. To deliver these prehospital care needs to become more efficient. Aim To develop a framework to support improved delivery of prehospital care. The recommendations are aimed at clinicians involved in prehospital and emergency health systems who will often not be stroke specialists but need clear guidance as to how to develop and deliver safe and effective care for acute stroke patients. Methods Building on the successful implementation program from the Global Resuscitation Alliance and the Resuscitation Academy, the Utstein methodology was used to define a generic chain of survival for Emergency Stroke Care by assembling international expertise in Stroke and Emergency Medical Services (EMS). Ten programs were identified for Acute Stroke Care to improve survival and outcomes, with recommendations for implementation of best practice. Conclusions Efficient prehospital systems for acute stroke will be improved through public awareness, optimized prehospital triage and timely diagnostics, and quick and equitable access to acute treatments. Documentation, use of metrics and transparency will help to build a culture of excellence and accountability.
Changing trends in the use of novel oral anticoagulants and warfarin for treating non-valvular atrial fibrillation
Authors: Birkinshaw Alexander , Fry Christopher H , Fluck David , Sharma Pankaj , Han Thang S 
Published: March 2020

Background Prevention of thromboembolism by novel oral anticoagulants is increasing, whilst use of vitamin K antagonists is on the decline. We assessed changes in the use of these anticoagulants in treating non-valvular atrial fibrillation between 2014 and 2018. Methods One-hundred and sixty-two consecutive patients (95 men, 67 women) with non-valvular atrial fibrillation, mean age 72.3 years (standard deviation = 11.0), underwent cardiac assessment in a single cardiac unit. Use of anticoagulants at the time of investigation was documented: overall 83 (51.2%) patients were prescribed novel oral anticoagulants and 79 (48.8%) warfarin treatment. Trends in treatment rates with either anticoagulant class over time were characterised by calculating the average annual percentage change using a Joinpoint Regression Program 4.7.0.0. Results There were diverging trends in anticoagulant treatment from 2014 to 2018 without join points: yearly increase in novel oral anticoagulant treatment (41.9, 45.5, 53.7, 53.1 and 72.7%, average annual percentage change = 16.2%, 95% confidence interval = 5.8% to 27.5%, p < 0.001), and decrease in warfarin treatment (57.1, 54.5, 46.3, 46.9 and 27.3%, average annual percentage change = −14.4%, 95% confidence interval = −25.2% to −2.1%, p < 0.001). Conclusions Changing trends in treatment with anticoagulants for patients with non-valvular atrial fibrillation observed within less than two years provide important information to healthcare services to estimate future pharmaco-economic costs for such treatments.
Suppurative arthritis of hip in a walking child: Effect of patient’s age, delay in surgical drainage, and organism virulence
Authors: Agarwal Anil , Kumar KH Vimal 
Published: March 2020

Introduction: We undertook a retrospective study to stratify the effect of patient’s age, delay in surgical drainage, and Staphylococcus aureus (SA) virulence on expected clinicoradiological outcomes following suppurative arthritis of hip joint in a walking child. Materials and methods: The study was conducted in 18 children over 2 years of age who suffered from joint fluid culture-positive SA arthritis of hip joint and treated with open arthrotomy. Outcomes were defined on the following criteria: age of child (≥5 years or <5 years), duration of symptoms elapsed before surgical intervention (≥5 days or <5 days), and virulence of SA (methicillin-resistant Staphylococcus aureus (MRSA)/methicillin-sensitive Staphylococcus aureus)). The final clinical results were graded according to Moon’s clinical criteria and radiologically by types described by Choi et al. Results: The mean patient age was 6.22 years at the time of index procedure. The average delay in surgery was 17.06 days. Follow-up averaged 15.28 months. Five patients had associated with osteomyelitis femur in preoperative radiographs. MRSA culture was grown in 8 hips (44.4%) of 18 hips. Unsatisfactory clinical results according to Moon’s criteria were obtained in 55.6% patients with 38.9% being MRSA infected. Unsatisfactory Choi’s radiological results were present in 33.3% patients, 27.8% under MRSA category. Our study did not find age to be a significant factor either in clinical or radiological outcome. The clinical results were unsatisfactory in 81.8% patients with surgical delays when compared to early surgery ( p < 0.05). Conclusions: The clinicoradiological outcome in suppurative hip arthritis of walking children was unsatisfactory when there was delay in treatment beyond 5 days, and the causative organism was virulent (MRSA). The presence of preoperative joint incongruency or osteomyelitis of proximal femur was another risk factor.
Glenoidplasty With Posterior Labral Reattachment for Posterosuperior Glenoid Impingement
Authors: Nourissat Geoffroy , Provost Julien , Vigan Marie , Cammas Claire 
Published: March 2020

Background: Posterosuperior glenoid impingement (PSGI), also known as shoulder internal impingement, is a cause of shoulder pain in athletes involved in overhead-throwing sports. PSGI is a condition mostly treated by rehabilitation. Surgery is indicated after unsatisfactory nonoperative management. However, with most of the surgical techniques proposed, the shoulder remains persistently painful during sport activity. Hypothesis: We aimed to evaluate the efficiency of adding posterior labral reattachment to glenoidplasty as a surgical treatment for PSGI. Our hypothesis was that posterior labral reattachment would have a positive effect on shoulder pain. To our knowledge, this combined procedure has not been previously described. Study Design: Case series; Level of evidence, 4. Methods: We retrospectively reviewed 11 male patients with PSGI treated with glenoidplasty and posterior labral reattachment at a single institution during a 7-year period and with a minimum 30-month follow-up after surgery. Most patients were recreational athletes, with 5 of them practicing at the national level and 1 being a professional athlete. Patients completed an online questionnaire consisting of the Simple Shoulder Test (SST), the Kerlan-Jobe Orthopaedic Clinic (KJOC) shoulder and elbow questionnaire, and a specific PSGI survey. Pre- and postoperative shoulder pain were analyzed using the paired t test, with significance set at P < .05. Results: All 11 patients investigated sought surgery because of persistent shoulder pain while practicing their sport. The patients underwent glenoidplasty and posterior labral fixation, and data for 9 patients were available for analysis (mean age, 29 years; range, 24-42 years). At a minimum 30-month follow-up, most patients (77.8%) showed decreased pain, and 8 patients were able to return to their previous sports activities, with 7 returning at the same or higher level. There was 1 patient who had to change sports because of a lack of force, while a second patient temporarily changed sport and then returned to his sport but at an inferior level. Pre- and postoperative pain differed significantly ( P < .05). Surgery did not impair daily life in all patients. Conclusion: Glenoidplasty associated with posterior labral reattachment significantly diminished shoulder pain in athletes involved in throwing sports. Most patients were able to return to their previous sport at the same or higher level. Posterior labral fixation may improve the benefit of glenoidplasty by decreasing pain.
Establishing User Error on the Patient-Reported Component of the American Shoulder and Elbow Surgeons Shoulder Score
Authors: Bourget-Murray Jonathan , Frederick Ariana , Murphy Lisa , French Jacqui , Barwood Shane , LeBlanc Justin 
Published: March 2020

Background: The American Shoulder and Elbow Surgeons (ASES) score is a patient-reported outcome (PRO) questionnaire developed to facilitate communication among international investigators and to allow comparison of outcomes for patients with shoulder disabilities. Although this PRO measure has been deemed easy to read and understand, patients may make mistakes when completing the questionnaire. Purpose: To evaluate the frequency of potential mistakes made by patients completing the ASES score. Study Design: Cross-sectional study; Level of evidence, 3. Methods: A prospective cross-sectional study was performed for 600 ASES questionnaires completed by patients upon their first visit to 1 of 2 clinic locations (Australian vs Canadian site). Two categories of potential errors were predefined, and then differences in error rates were compared based on demographics (age, sex, and location). To determine whether these methods were reliable, an independent, third reviewer evaluated a subset of questionnaires separately. The interrater reliability was evaluated through use of the Cohen kappa. Results: The mean patient age was 49.9 years, and 63% of patients were male. The Cohen kappa was high for both evaluation methods used, at 0.831 and 0.918. On average, 17.9% of patients made at least 1 potential mistake, while an additional 10.4% of patients corrected their own mistakes. No differences in total error rate were found based on baseline demographics. Canadians and Australians had similar rates of error. Conclusion: To ensure the accuracy of the ASES score, this questionnaire should be double checked, as potential mistakes are too frequently made. This attentiveness will ensure that the ASES score remains a valid, reliable, and responsive tool to be used for further shoulder research.
Revision Arthroscopic Bankart Repair in the Military
Authors: Slaven Sean E. , Tardif Robert , Foley Kevin , Cameron Kenneth L. , Posner Matthew A. , Dickens Jonathan F. 
Published: March 2020

Objectives: There is no consensus on the optimal method of stabilization (arthroscopic or open) for revision anterior shoulder stabilization. The purpose of this study was to determine the success of revision arthroscopic stabilization at preventing further recurrence in active duty military patients. Methods: 53 revision arthroscopic stabilizations were performed at our institution between 2005-2016 for recurrent anterior shoulder instability after an arthroscopic Bankart index procedure. Shoulders with glenoid bone loss >20% were excluded from the study. The primary outcome of interest was the ability to return to activity/duty without subsequent instability. Patients were followed for time to a subsequent instability event and repeat revision arthroscopic stabilization following return to duty/activity. Results: Patient age at revision surgery averaged 22.9 ± 4.3 years. Mean follow up was 6.1 years (range 0.4-12.9). 34 out of 53 patients (64%) returned to duty without recurrent instability following revision arthroscopic anterior stabilization. 19 patients (36%) experienced recurrent instability following return to duty after revision arthroscopic stabilization. Glenoid bone loss averaged 7.8% ± 8.0% in the successful group and 6.5 % ± 6.5% in the failure group (p=0.573). Durability of the index surgery was significantly longer in the successful group (38.1 ± 31.3 months vs. 20.5 ± 17.8 months, p=.029). There was no difference between groups in patient age or number of anchors used in the index or revision stabilization procedures. Conclusion: Revision arthroscopic stabilization of failed primary arthroscopic Bankart repair has a failure rate of 36% in a young active duty military population, which is substantially higher than primary arthroscopic Bankart repair in this population and higher than revision arthroscopic Bankart repair in other patient populations. The similar amounts of bone loss between groups indicates that bone loss is not the primary determinant of failure in revision arthroscopic stabilization.
Quantitative proteomics analysis of differentially expressed proteins induced by astragaloside IV in cervical cancer cell invasion
Authors: Xia Chenglai , He Zhihong , Cai Yantao 
Published: March 2020

Abstract
Background
Cervical cancer remains the second leading cause of mortality in women in developing countries. While surgery, chemotherapy, radiotherapy, and vaccine therapy are being applied for its treatment, individually or in combination, the survival rate in advanced cervical cancer patients is still very low. Traditional Chinese medicine has been found to be effective in the treatment of cervical cancer. Astragaloside IV (AS-IV), a compound belonging to Astragalus polysaccharides, shows anticancer activity through several cell signaling pathways. However, the detailed molecular mechanism governing the anticancer activity of AS-IV remains unknown.

Material and methods
In our study, we performed tumor xenograft analysis, transwell cell migration and invasion assay, Western blot analysis, and iTRAQ combination by parallel reaction monitoring (PRM) analysis to study the molecular mechanism of AS-IV in the suppression of cervical cancer cell invasion.

Results
Our results showed that AS-IV suppressed cervical cancer cell invasion and induced autophagy in them, with the tumor growth curve increasing slowly. We also identified 32 proteins that were differentially expressed in the SiHa cells when treated with AS-IV, with 16 of them involved in the upregulation and 16 in the downregulation of these cells. These differentially expressed proteins, which were predominantly actin–myosin complexes, controlled cell proliferation and cell development by steroid binding and altering the composition of the cell cytoskeleton. DCP1A and TMSB4X, the two proteins regulating autophagy, increased in cervical cancer cells when treated with AS-IV.

Conclusions
We conclude that AS-IV could inhibit cervical cancer invasion by inducing autophagy in cervical cancer cells. Since iTRAQ combination by PRM has been observed to be useful in identifying macromolecular target compounds, it may be considered as a novel strategy in the screening of anticancer compounds used in the treatment of cervical cancer.
Mixture toxicity in the Erft River: assessment of ecological risks and toxicity drivers
Authors: Markert Nele , Rhiem Stefan , Trimborn Michael , Guhl Barbara 
Published: March 2020

Abstract
Background
A vast number of chemical substances are released into the aquatic environment, leading to complex chemical mixtures in surface waters. Current water quality assessments, however, are based on the risk assessment of single substances. To consider potential mixture effects in water quality assessments, the North Rhine Westphalian State Agency for Nature, Environment and Consumer Protection (LANUV), Germany started a project assessing mixture toxicity in surface waters. This article summarises the mixture evaluation of chemical data collected by the Erftverband during a water sampling campaign in the Erft River in 2016/2017. Altogether, 153 substances were included in the analysis, of which 98 were detected. Two different approaches based on the concept of concentration addition were used to analyse the data. The results were compared to findings based on datasets from LANUV surveillance monitoring according to the EU Water Framework Directive.

Results
Acute and chronic mixture risk calculations indicated risks for 32% and up to 90% of the samples, respectively. The greatest acute toxic pressure was identified for the aquatic flora due to continuous exposure to varying pesticides, whereas the greatest chronic mixture risk was identified for fish as result of a ubiquitous presence of the pharmaceuticals diclofenac and ibuprofen. Overall, only a limited number of substances significantly contributed to the calculated mixture risks. However, these substances varied seasonally and regionally. When mixture risks were calculated based on different datasets, the monitoring design markedly affected the outcome of the mixture risk assessment. Data gaps of both ecotoxicological and exposure data lead to high uncertainties in the mixture risk assessment.

Conclusions
Ecotoxicological effects on aquatic organisms caused by chemical mixtures can be expected along the Erft River throughout the year. Both mixture risk assessment approaches can be used for a conservative assessment of mixture risks and characterise the aquatic pollution in the river more realistically than single substance assessments. For the integration of mixture risk evaluations into the assessment and management of the chemical water quality, a two-staged assessment combining both approaches is suggested. To improve future risk assessments, the accessibility and exchange of high-quality ecotoxicological data should be enhanced.
Effect of coronary artery bypass grafting on blood pressure response to head-up tilting
Authors: Hori Shinnosuke , Kamijo Yoshi-ichiro , Yuzaki Mitsuru , Kawabe Tetsuya , Minami Kohei , Umemoto Yasunori , Yokoyama Mao , Uenishi Hiroyasu , Nishimura Yoshiharu , Kouda Ken , Mikami Yukio , Tajima Fumihiro 
Published: March 2020

AbstractBlood pressure response to head-up tilt (HUT) in 7 healthy subjects and 9 patients before and after coronary artery bypass grafting (CABG) was measured during supine and 15-min 60° HUT. Stroke volume (SV) and ejection fraction (EF) were assessed by echocardiography. Baseline mean arterial pressure (MAP) and heart rate (HR) in patients before CABG were similar to healthy subjects. MAP in patients decreased by 6 (4–9) mmHg [median (1st–3rd quartiles)] during 7–12 mmHg of HUT with decreased cardiac output (CO = SV × HR) while HR remained unchanged. MAP in healthy subjects remained unchanged during HUT with increased HR. Body weight decreased by 3.5 (2.5–3.7) kg and MAP decreased by 6 (2–13) mmHg during the last 3-min HUT while HR increased after CABG. Decreases in SV and CO during HUT disappeared after CABG. Blood pressure decreased during HUT in patients before and after CABG regardless of HR response.
Robust high-throughput assays to assess discrete steps in ubiquitination and related cascades
Authors: Fenteany Gabriel , Gaur Paras , Sharma Gaurav , Pintér Lajos , Kiss Ernő , Haracska Lajos 
Published: March 2020

Abstract
Background
Ubiquitination and ubiquitin-like protein post-translational modifications play an enormous number of roles in cellular processes. These modifications are constituted of multistep reaction cascades. Readily implementable and robust methods to evaluate each step of the overall process, while presently limited, are critical to the understanding and modulation of the reaction sequence at any desired level, both in terms of basic research and potential therapeutic drug discovery and development.

Results
We developed multiple robust and reliable high-throughput assays to interrogate each of the sequential discrete steps in the reaction cascade leading to protein ubiquitination. As models for the E1 ubiquitin-activating enzyme, the E2 ubiquitin-conjugating enzyme, the E3 ubiquitin ligase, and their ultimate substrate of ubiquitination in a cascade, we examined Uba1, Rad6, Rad18, and proliferating cell nuclear antigen (PCNA), respectively, in reconstituted systems. Identification of inhibitors of this pathway holds promise in cancer therapy since PCNA ubiquitination plays a central role in DNA damage tolerance and resulting mutagenesis. The luminescence-based assays we developed allow for the quantitative determination of the degree of formation of ubiquitin thioester conjugate intermediates with both E1 and E2 proteins, autoubiquitination of the E3 protein involved, and ubiquitination of the final substrate. Thus, all covalent adducts along the cascade can be individually probed. We tested previously identified inhibitors of this ubiquitination cascade, finding generally good correspondence between compound potency trends determined by more traditional low-throughput methods and the present high-throughput ones.

Conclusions
These approaches are readily adaptable to other E1, E2, and E3 systems, and their substrates in both ubiquitination and ubiquitin-like post-translational modification cascades.
Exogenous supply of Hsp47 triggers fibrillar collagen deposition in skin cell cultures in vitro
Authors: Khan Essak S. , Sankaran Shrikrishnan , Llontop Lorena , del Campo Aránzazu 
Published: March 2020

Abstract
Background
Collagen is a structural protein that provides mechanical stability and defined architectures to skin. In collagen-based skin disorders this stability is lost, either due to mutations in collagens or in the chaperones involved in collagen assembly. This leads to chronic wounds, skin fragility, and blistering. Existing approaches to treat such conditions rely on administration of small molecules to simulate collagen production, like 4-phenylbutyrate (4-PBA) or growth factors like TGF-β. However, these molecules are not specific for collagen synthesis, and result in unsolicited side effects. Hsp47 is a collagen-specific chaperone with a major role in collagen biosynthesis. Expression levels of Hsp47 correlate with collagen deposition. This article explores the stimulation of collagen deposition by exogenously supplied Hsp47 (collagen specific chaperone) to skin cells, including specific collagen subtypes quantification.

Results
Here we quantify the collagen deposition level and the types of deposited collagens after Hsp47 stimulation in different in vitro cultures of cells from human skin tissue (fibroblasts NHDF, keratinocytes HaCat and endothelial cells HDMEC) and mouse fibroblasts (L929 and MEF). We find upregulated deposition of fibrillar collagen subtypes I, III and V after Hsp47 delivery. Network collagen IV deposition was enhanced in HaCat and HDMECs, while fibril-associated collagen XII was not affected by the increased intracellular Hsp47 levels. The deposition levels of fibrillar collagen were cell-dependent i.e. Hsp47-stimulated fibroblasts deposited significantly higher amount of fibrillar collagen than Hsp47-stimulated HaCat and HDMECs.

Conclusions
A 3-fold enhancement of collagen deposition was observed in fibroblasts upon repeated dosage of Hsp47 within the first 6 days of culture. Our results provide fundamental understanding towards the idea of using Hsp47 as therapeutic protein to treat collagen disorders.