bluuebunny/crossref_metadata_2025_split huggingface dataset:
Secernin 1 promotes cell invasion and migration by activating the TGF-β/Smad3 pathway in oral squamous cell carcinomas
Authors: Xiao Li , Wen Shuqiong , Zhang Qianyu , Peng Yanshuang , Zheng Kaiyue , Yang Lianjie , Wang Zhi , Liu Ying 
Published: January 2020

Abstract
Background Secernin-1 (SCRN1) is a regulator of exocytosis in mast cells. Recently, SCRN1 has been reported to be correlated with the prognosis of colorectal cancer and gastric cancer but its functional effects on oral squamous cell carcinoma (OSCC) remains unclear. Our aim was to explore the expression pattern and the migration and invasion effects of the newly identified SCRN1 in OSCC. Methods Western blotting (WB) was used to measure SCRN1 expression in human OSCC tissue samples and OSCC cell lines. Then, the effects of SCRN1 on OSCC cell proliferation, invasion and metastasis were analyzed by cell counting kit-8 (CCK-8) and transwell assays. The secretion of matrix metalloproteinase (MMP)-2 and MMP-9 in SCRN1 knockdown OSCC cells and vector cells was investigated by enzyme-linked immunosorbent assay (ELISA). The expression levels of TGF-β, Smad3 and phosphorylated Smad3 (p-Smad3) in SCRN1 knockdown OSCC cells and vector cells were measured by WB. Results The expression of SCRN1 was significantly elevated in the OSCC tissues and cell lines. Furthermore, SCRN1 knockdown attenuated the proliferation, migration and invasion of SCC15 and HSC3 cells. SCRN1 knockdown reduced the secretion of MMP-9 from HSC3 and SCC15 cells, but the secretion of MMP-2 did not obviously change. Additionally, SCRN1 knockdown reduced the expression of TGF-β and p-Smad3 in HSC3 and SCC15 cells. Conclusions Our study demonstrated that SCRN1 is upregulated in OSCC. Further studies demonstrated that SCRN1 promotes proliferation, invasion and metastasis of OSCC cells via TGF-β/Smad3 signaling.
Prevalence and Risk Factors of Periodontal Disease among Pregnant Women in a Rural Area of China
Authors: Chen Mengqi , Su Yi , Xiong Xu , Peng Jinxiang , Zhu Xiaoling , Wang Jinhua , Jiang Hong , Qian Xu 
Published: January 2020

Abstract
BackgroundPeriodontal disease is one of the most important common oral diseases contributing to the global burden of chronic disease. Systematic reviews have demonstrated that poor periodontal condition during pregnancy was significantly associated with adverse pregnancy outcomes such as preterm birth, low birth weight, preeclampsia, etc. Oral health care is often a neglected area in low- and middle- income countries, particularly in rural areas due to the limited health human resources and poor infrastructures resources. Very few studies have explored the risk factors of periodontal disease among pregnant women in rural areas of China.MethodsA cross-sectional study was conducted among 648 women between 2 and 28 weeks of gestation at an antenatal clinic in Maternal and Child Health Hospital of Leping, Jiangxi Province, China. Periodontal Screening & Recording (PSR) was used to determine periodontal disease. Data on respondents’ demographic characteristics, gestational age, regular oral health habits, and mental health condition were collected using a self-administered questionnaire. ResultsThe prevalence of periodontal disease in pregnant women was 79.78% (95% CI: 76.7%~82.7%). Periodontal disease was significantly associated with increased maternal age (aOR =1.13, 95%CI: 1.06~1.21, P<0.001) and gum bleeding during tooth brushing (aOR =3.76, 95%CI: 2.05~7.00, P<0.001). The severity of periodontal disease advanced with the increased maternal age and gum bleeding during pregnancy. ConclusionsPregnant women with increased maternal age and gum bleeding during tooth brushing were at a higher risk of periodontal disease and needed to have the access to oral health care.
Oral impacts mediate the association between reduced dentition and self-perceived need for complete dentures
Authors: Campos Fernanda Lamounier , Kawachi Ichiro , Rhodes Gabriela Aparecida Caldeira , Sampaio Aline Araujo , Chalub Loliza Luiz Figueiredo Houri , Ferreira Raquel Conceicao 
Published: January 2020

Abstract
Background: To evaluate the direct association and oral impact-mediated association between a reduced dentition and the self-perceived need for complete dentures (CD) in dentate adults. Methods: Data from the Brazilian National Oral Health Survey (2010) were analyzed. Self-perceived need for complete dentures was investigated by the question "Do you think you need to wear CD?". The hierarchical dental functional classification defined reduced dentitions: at least one tooth in each arch, 10 teeth in each arch, 12 anterior teeth, posterior occluding pairs (POPs) of premolars (≥3) and molars (≥1 bilateral). Presence of all these criteria were defined as functional dentition (FDClassV). Oral impacts were assessed using the Oral Impacts on Daily Performances scale. Associations were investigated using logistic regression and structural equations. Results: FD(ClassV) was associated with less self-perceived need for CD both directly (p<0.001) and mediated by oral impacts (p=0.003). Dentitions without molar POPs were associated with the outcome mediated by oral impacts (p=0.002). Individuals with 10 teeth in each arch (with or without anterior teeth and POPs) had similar frequencies of self-perceived denture needs. Individuals with <10 teeth in each arch and 12 posterior teeth did not have a higher frequency of self-perceived denture need. Conclusions: Oral impacts mediated the association between reduced dentitions and self-perceived denture needs. Clinical relevance: Individuals with tooth loss may report need for CD, even when they have dental configurations compatible with functionality. The dentist must taken into account patient-centered measures to make decision about oral health prevention and treatment.
Evaluation of vascular cell adhesion molecule-1, intracellular cell adhesion molecule-1 and E-selectin levels in preeclampsia: A Systematic Review and Meta-Analysis.
Authors: Li Xueli , Tang Qian , Liu Shiguo , Xu Yinglei , Li Miaomiao , Cao Caixia , Zhang Lu 
Published: January 2020

Abstract
BackgroundSoluble adhesive molecules are potential mediators of endothelial dysfunction in preeclampsia. The purpose of this study was to examine the relationship between pre-eclampsia and maternal circulation vascular cell adhesion molecule-1 (VCAM-1), Intracellular cell adhesion molecule-1(ICAM-1) and E-selectin.MethodsVCAM-1, ICAM-1 and E-selectin studies of maternal circulation in preeclampsia and normal pregnant women were reported by systematic literature search in PubMed (Medline), with 21 eligible reports. The standardized mean difference (MD) and their 95% confidence intervals (CIs) were calculated as random effect analysis of association among studies.ResultsPreeclampsia is associated with elevated VCAM-1 [mean difference (MD) = 1.17 ng/ml, 95% confidence interval (CI) 0.85-1.50 ng/ml], and E-Selectin (MD = 2.18ng/ml, 95% CI 1.16-3.20ng/ml), but not associated with ICAM-1 (MD= 0.43ng/ml, 95% CI -0.03-0.90ng/ml). In the subgroup analysis of mild and severe preeclampsia, VCAM-1, ICAM-1 and E-selectin all showed increased levels compared with normal pregnancy women.ConclusionThe meta-analyses have shown elevated VCAM-1 and E-selectin levels in the circulation of pregnant women with preeclampsia.
The DPP-4 inhibitor sitagliptin alters the gut microbiota and prevents neuronal damage of hippocampus in STZ induced type 2 diabetic rats
Authors: Chang Ying , Xiang Ting , Wang Hongyan , Zhou Fanlin , Liang Jie , Deng Wuquan , Li Shengbing , Chen Wenwen , Li Ke , Li Yu , Liu Dongfang , Yang Gangyi , Tian Mingyuan 
Published: January 2020

Abstract
Background : Type 2 Diabetes mellitus is characterized by impaired glucose metabolism and insulin resistance with increasing risk of specific complication, including central neuropathy. Dipeptidyl peptidase 4 (DPP-4) inhibitors are agents designed to regulate hyperglicemia through increasing the half-life of incretins. Although they are administered orally, their effects on central neuropathy and gut microbiota remain unclear. Our objective was aimed to identify the impact of the DPP-4 inhibitor sitagliptin on gut microbiota and central neuropathy in a rat model of streptozotocin (STZ)-induced diabetes
Methods: Diabetic rats were induced by a high-fat diet and streptozotocin injection. Diabetic rats were orally administered a dose of sitagliptin (10mg/kd/d), and normal diet for 12 weeks. Fecal DNA extraction and sequencing based on analysis of 16S rRNA genes was used to determine the overall structure of microbiota in fecal samples. Then rats were killed, of which the brain tissue sections were observed for changes of neurons in the hippocampus by HE staining.
Results: Sequencing of the V3-V4 regions of 16S rRNA genes revealed that sitagliptin significantly altered the gut microbiota. Compared to the diabetic group, Shannon index and the abundance of phylum Bacteroidetes decreased significantly, whereas the proportion of phylum Firmicutes increased significantly in the sitagliptin-treated rats. Moreover, higher abundance of a few putative short-chain fatty acid-producing bacteria, including Clostridium and Fusobacterium was observed remarkably after sitaglitin treatment. Genera Lactobacillus , Streptococcus and Clostridium, which could produce some neurotransmitters, were also found increased substantially in the sitagliptin-treated rats. Notably, we found that sitagliptin treatment could ameliorate the abnormal changes of pyramidal neurons in hippocampus with HE staining.
Conclusions: Our study demonstrated that sitagliptin prevented the neuronal damage of hippocampus in type 2 diabetic rats, accompanied by changes of gut microbiome. It indicated that sitagliptin might alleviate diabetic central neuropathy through triggering “microbiota-gut-brain” axis.
Developing a Machine Learning prediction model for bedside decision support by predicting readmission or death following discharge from the Intensive Care unit
Authors: Thoral Patrick J. , Fornasa Mattia , Bruin Daan P. de , Hovenkamp Hidde , Driessen Ronald H. , Girbes Armand R.J. , Hoogendoorn Mark , Elbers Paul W.G. 
Published: January 2020

Abstract
Background Unexpected ICU readmission is associated with longer length of stay and an increase in mortality. Real time support systems could prevent untimely discharge from the ICU. We aim to develop a machine learning model for implementation at the bedside by predicting the risk of ICU readmission or death at time of potential discharge, showing feature importance and visualizing day-to-day changes in risk. Methods Data from adult patients, admitted to our mixed surgical-medical ICU between 2004 and 2016, were used in the analysis. Patient characteristics, clinical observations, (automated) physiological measurements, laboratory studies and treatment data were considered as model features. Different supervised learning algorithms were trained to predict ICU readmission and/or death, both within 7 days from ICU discharge, using 10-fold cross-validation. Feature importance was determined using SHapley Additive exPlanations. We constructed readmission probability-time curves to identify subgroups. Results Our dataset included 14,105 admissions. The combined readmission/mortality rate within seven days of ICU discharge was 5.3%. Using Gradient Boosting, the model achieved a Receiver Operating Characteristic AUC of 0.802 (95% CI 0.789-0.816) and a Precision-Recall AUC of 0.198 (95% CI 0.185-0.211). The most predictive features were well-known parameters, including physiological parameters, as well as less apparent features like nutritional support. Impact analysis using probability-time curves identified specific patients groups, that might lead to a change in discharge management with a relative risk reduction of 17%. Conclusions We developed a model that can accurately predict readmission and mortality after ICU discharge. Impact analysis showed that a relative risk reduction of 17% could be achievable. Given the large and increasing number of ICU admissions worldwide, this modest reduction may have significant impact for patients and society.
Structural equation modelling the ‘control of gut overgrowth’ in the prevention of ICU acquired Gram-negative infection.
Authors: Hurley James C 
Published: January 2020

Abstract
Background: Conceptually, the ‘control of gut overgrowth’ (COGO), including ‘abnormal Gram-negative bacilli’ (AGNB), is key to the mediation of infection prevention by Selective Digestive Decontamination (SDD). However, the relative importance of the SDD components; topical (TAP), enteral (EAP) and protocolized parenteral antibiotic prophylaxis (PPAP), versus other methods of infection prevention and versus other contextual exposures cannot be resolved within individual studies. Methods: Generalized structural equation models (GSEM) based on COGO concepts were confronted with data derived from >200 infection prevention studies reporting incidences of overall, Pseudomonas and Acinetobacter bacteremia as well as ventilator associated pneumonia (VAP) data including the following group level exposures; TAP, EAP and PPAP use versus antiseptic versus non-decontamination mode of infection prevention; proportion receiving mechanical ventilation (MV); trauma ICU; mean length of ICU stay and concurrency versus non-concurrency of TAP study control groups. Results: In GSEM modelling of Pseudomonas and Acinetobacter gut overgrowth (GO) as latent variables, anti-septic interventions had the strongest negative effect against Pseudomonas GO but no intervention was significantly negative against Acinetobacter GO. Strikingly, PPAP and concurrency each have positive effects in the model, EAP is neutral and Acinetobacter bacteremia incidences are high within TAP studies, moreso with PPAP exposure. Paradoxically, TAP (moreso with PPAP) appears to provide the strongest summary prevention effects against bacteremia and VAP overall. Conclusions: GSEM modelling of published data provides novel insights into the COGO concept and the complex and profoundly paradoxical relationships between various interventions, concurrency and other exposures in relation to infection with AGNB.
Designing a tool for measuring the awareness of predisposing factors and dimensions of low back pain in operating room personnel and evaluating its psychometric properties
Authors: Tavakkol Reza , Eslami Jamshid , Zarshenas Ladan , Amiri Azadeh 
Published: January 2020

Abstract
Background: Low back pain is the most common type of work-related musculoskeletal disorders and one of the costliest health problems. This study aimed to design a psychometric tool to measure the operating room personnel’s awareness of predisposing factors and dimensions of low back pain. Methods: This methodological study aimed to design a psychometric tool for assessing the operating room personnel’s awareness of predisposing factors and dimensions of low back pain in. In the first step, the main dimensions and concepts were developed by reviewing the literature and consulting with professors and experts. In the second step, the initial items of the questionnaire were formulated. Finally, the psychometric properties of the questionnaire were determined using face, content and construct validity, internal consistency, and test-retest reliability. Results: In the first phase of the study, the concept of low back pain and its risk factors were determined, and then the initial items of the tool were developed. In the next step, face and content validity of the instrument were determined both qualitatively and quantitatively (content validity ratio=0.91 and content validity index=0.95). Indeed, construct validity was assessed by convergent and divergent validity using chronic pain and general health questionnaires, respectively (correlation coefficient in convergent validity=0.70 and correlation coefficient in divergent validity=-0.37). Finally, the reliability of the instrument was determined using internal consistency (α=0.60) and test-retest reliability (0.77). Conclusion: Considering the design, validity, and reliability of the instrument for measuring the awareness of predisposing factors and dimensions of low back pain in operating room personnel, we suggest that it should be used for evaluation of the awareness and different dimensions of low back pain among the operating room personnel.
COMBINED HORMONAL CONTRACEPTION AND NOT ONLY…
Authors: Erofeeva L. V. 
Published: January 2020

Chlormadinone acetate-containing combined contraceptives are well researched, they have a high degree of reliability during prolonged use in different modes. They have a protective anti-atherogenic properties and is offset by the risk for cardiovascular system, because the existing data indicate that the risk of thromboembolism against the background of chlormadinone preparation is minimal to date among all COCs and is comparable with the «first-generation» progestins, like levonorgestrel. The undeniable advantage of CMA-containing COC is a neutral impact on carbohydrate metabolism and blood coagulation system. CMA COCs have anti-atherogenic effect, which makes them “the drugs of choice” in patients with sclerocystic ovaries, metabolic syndrome and systemic diseases associated with hypercoagulability.Russian scholars and Western specialists who studied the effect of CMA-containing combination drug, allow us to recommend COC Belara® to assign not only with the contraceptive purpose, but also as a product for the stability of the menstrual cycle, as the drug of choice in the hyperpolimenorrhea, al’go – and dysmenorrhea, hyperandrogenism, expressed in the form of acne, hirsutism, oily seborrhea, decrease in sexual activity because of dyspareunia, as well as to anxiolytic and sedative effect in PMS.
VITAMINS FOR PREGNANT, ACTIVE FOLATES WITH 100% ABSORPTION
Authors: Khilkevich E. G. , Yazykova O. I. 
Published: January 2020

Study objective. To study the efficacy of folic acid drugs, in particular metafolin, at the stage of pregravid preparation and during pregnancy to prevent congenital malformations and complications of pregnancy.Material and methods. We conducted a search of the literature sources by the keywords «folic acid», «folates», «metafolin», «pregnancy», «methyltetrahydrofolate» in domestic and foreign databases: elibrary, Medline/PubMed, Embase, CINAHL. 23 sources were selected.Study results. One of the main challenges that the obstetrician faces is the prevention of pregnancy complications and congenital malformations of the fetus. In the literature there is considerable amount of evidence about the effectiveness of folic acid. However, the natural food folates and folic acid preparations may not be absorbed to a sufficient degree by the body in women with defects in folate-converting enzymes, the incidence rate of which reaches 50%.Conclusion. All women at the stage of pregravid preparation and during pregnancy should take folates to prevent complications of pregnancy and congenital malformations in the fetus. Given the high prevalence of mutations in genes responsible for folateconverting enzymes, in clinical practice it is recommended to use folates able to be absorbed by the body regardless of genetic variants of the folate cycle enzymes.
MONITORING THE RESISTANCE OF CLINICAL STRAINS OF CANDIDA YEAST TO FLUCONAZOLE (A BRIEF LITERATURE REVIEW)
Authors: Zheltikova T. M. 
Published: January 2020

The article analyzes the global and national research on the resistance of Candida yeasts to fluconazole. The studies demonstrate that the formation of resistance is determined by many factors: type of yeast, choice of the antimycotic medication, geographical location, etc. In addition, one can not disregard the socio-economic and even political causes. The frequency of detection of drug-resistant strains of different species of Candida yeast to fluconazole varies across different regions, between countries of the same region, and may vary from year to year within a country. In other words, the formation of yeast resistance/ susceptibility to fluconazole, and to other antifungals alike, is dynamic and may be reversible.Therefore, both global and national studies conducted over the past decades and devoted to the formation of resistance of Candida yeast to azoles, in particular, fluconazole, have shown that it is still the medication of choice for the treatment of candidiasis, including acute vulvovaginal candidiasis, as well as for relief and prevention of exacerbations of recurrent vulvovaginal candidiasis. C. krusei was and remains one of the most fluconazole-resistant yeast species. Other species, such as C. inconspicua and C. norvegensis, the number and incidence of which is too low for the moment to make any statistically valid conclusions, may in the future be added to the list.
EVALUATION OF THE EFFECTIVENESS OF ALTERNATIVE TREATMENTS FOR MENOPAUSAL SYMPTOMS IN POSTMENOPAUSAL WOMEN
Authors: Yureneva S. V. , Ermakova E. I. 
Published: January 2020

Menopause is the final cessation of menstrual cycles due to loss of follicular activity of the ovaries. According to the results of a meta-analysis of 36 studies performed in 35 countries, the average age of menopause onset is 48.8 years (95% CI 48,3 – 49,2) with significant fluctuations of this indicator depending on the geographical region of residence of women: lower in Africa, Latin America and middle East countries (47,2–48.4 years) and later in Europe and Australia (50,5–51,2 year) [1]. The number of women in peri- and postmenopausal phase in connection with the increase in life expectancy is increasing. In Russia it is currently more than 21 million. The average age of the menopause onset in Russia ranges from 49 to 51 years, at the same time women live in conditions of estrogen deficit for 1/3 of their life [2, 3].
EARLY EXPERIENCE OF GPP-1 LIRAGLUTIDE 3.0 MG ANALOGUE IN RUSSIA AND ITS EFFECT ON METABOLIC RISK FACTORS IN OBESITY PATIENTS
Authors: Komshilova K. A. , Troshina E. A. , Ershova E. V. , Logvinova O. V. 
Published: January 2020

Obesity is a chronic disease associated with cardiometabolic risk factors of cardiovascular diseases and type 2 diabetes. In 2016, in Russia a new drug was registered for the treatment of patients with adiposity - liraglutide 3.0 mg, which is an analog of human glucagonoma peptide-1. The study purpose: to study the efficacy of liraglutide 3.0 mg/day and its impact on cardiometabolic risk factors in patients with obesity. We examined 30 patients aged 25-59 with obesity without diabetes. Patients received therapy with liraglutide 3.0 mg/day for 3 months. The examination included measurement of anthropometric parameters, blood sampling with the estimation of the parameters of lipid and carbohydrate metabolism.Results: after 3 months of treatment with liraglutide 3.0 mg a significant improvement of anthropometric parameters was observed: reduction of weight, BMI and waist circumference and a significant improvement in parameters of metabolic comorbidities.Conclusions: complex therapy with use of liraglutide 3.0 mg per day for 3 months is effective for therapy of obesity and correction of obesity-associated cardiometabolic disturbances.
DIABETES INSIPIDUS AND COMORBID VIOLATIONS OF FLUID-SALT BALANCE
Authors: Lukyanchikov V. S. 
Published: January 2020

Aim. To prove that formally low incidence and frequency of diabetes insipidus (ND) and other variants of syndrome poliuriapolydipsia (SPP) did not reflect the true prevalence and clinical significance of such disturbances.Material and methods. Demonstrated etiology and pathogenesis diversity of SPP, difficulties in its classification and diagnosis.Results and conclusions. Proposed diagnostic and therapeutic approaches.