bluuebunny/crossref_metadata_2025_split huggingface dataset:
Combining ability and testcross performance of low N tolerant intermediate maize inbred lines under low soil nitrogen and optimal environments
Authors: Ribeiro P.F. , Badu Apraku B. , Gracen V. , Danquah E.Y. , Afriyie-Debrah C. , Obeng-Dankwa K. , Toyinbo J.O. 
Published: September 2020

AbstractLow soil nitrogen (low N) threatens maize production in sub-Sahara Africa (SSA). We examined the mode of gene action conditioning grain yield of intermediate maturing inbreds and evaluated lines in hybrid combinations for high yield, stability and tolerance to low N. Thirty-two sets of inbreds were crossed to three elite testers (87036, 1368 and 9071) to generate 96 F1 hybrids. The testcrosses plus four hybrid checks were evaluated under low (30 kg/ha) and high (90 kg/ha) N environments at three locations for 2 years in Ghana. Significant general combining ability (GCA) and specific combining ability (SCA) effects were detected for grain yield and most measured traits across test environments, indicating that both additive and non-additive gene action governed the inheritance of the traits. GCA effects were greater than SCA effects, indicating that most traits were controlled predominantly by additive gene action and that inbreds with positive significant GCA effects for grain yield and other traits would contribute favourable alleles to progenies across environments. Hybrid CZL 0001 × 9071 possessed high GY, increased EPP, desirable EHT and PLHT and was the highest yielding under each of two research conditions. Significant genetic correlations were observed between GY and PLHT, EPP, EHT, CA and PA implying that improvement of these traits would lead to significant gains in grain yield under low-N conditions. Hybrids CLWN 247 × 9071, ZM523B-29-2-1-1-B*6 × 9071, TZD II 68 × 1368 and P43SCRq Fs100-1-1-8 × 9071 were high-yielding, stable and low-N tolerant and should be tested on-farm and commercialized.
Portfolio Choice: Familiarity, Hedging, and Industry Bias
Authors: Che Xin , Liebenberg Andre P. , Lynch Andrew A. 
Published: September 2020

AbstractInvestors may underdiversify their portfolios by overweighting securities in which they perceive an informational advantage or by underweighting securities to hedge risks outside the portfolio. We investigate underdiversification in institutional portfolio construction by examining the under/overweighting of industries in U.S. property–liability (PL) insurers’ equity portfolios. We find that PL insurers underweight both their own industry and highly correlated industries in their portfolios. This underweighting is larger for PL insurers exposed to higher underwriting risk. Although PL insurers have an informational advantage in investing in their peers, their underwriting risk drives them to underweight stocks in their industry.
‘Sometimes you gotta get out of your comfort zone’: retirement migration and active ageing in Cuenca, Ecuador
Authors: Hayes Matthew 
Published: September 2020

AbstractThe article extends notions of ‘active’ and ‘successful’ ageing by exploring the narratives of ageing in the retirement migration of Canadian and American older adults in Cuenca, Ecuador. The article is based on 83 semi-structured qualitative interviews (11 of which are follow-up interviews), most conducted in the first half of the 2010s. I explore how notions of finite time and imaginaries of a fourth age of decline and death inform the migration decisions and imaginaries of Canadian and American retirement migrants. I argue that their desire to seek self-expansive, new experiences through migration and contact with cultural difference dialogues with an increasingly competitive neoliberal culture of ageing, that emphasises success through activity, youthfulness and consumption. While there are certainly other ideals that help inform North American migration to Ecuador, I argue that these particular ideals illustrate how discourses of ‘active ageing’ have been taken up ‘from below’, by ageing North American adults, many of whom identify with the aspirations of policy and corporate discourses of activity and success, but who find themselves ageing into material conditions that preclude them. Migration to a lower-cost country, like Ecuador, helps them to experience these aspirations more positively, but may have uneven effects on lower-income workers and their ability to remain in place in the communities marketed for this type of migration.
Two Patients: Professional Formation before “Narrative Medicine”
Authors: FINS JOSEPH J. 
Published: September 2020

AbstractIn this essay, the author reflects on his development as a physician by recounting two patient narratives of patients he cared for as a third year medical student. In the process of telling these stories of sickness, the author also provides a window on medical practice in the 1980’s in an academic medicine center and how practices have changed. Decades before what has been dubbed “narrative medicine,” the author learned the power of words to shape relationships and promote professional formation.
Altering MYC phosphorylation in the epidermis increases the stem cell population and contributes to the development, progression, and metastasis of squamous cell carcinoma
Authors: Wang Xiaoyan , Langer Ellen M. , Daniel Colin J. , Janghorban Mahnaz , Wu Vivian , Wang Xiao-Jing , Sears Rosalie C. 
Published: September 2020

AbstractcMYC (MYC) is a potent oncoprotein that is subject to post-translational modifications that affect its stability and activity. Here, we show that Serine 62 phosphorylation, which increases MYC stability and oncogenic activity, is elevated while Threonine 58 phosphorylation, which targets MYC for degradation, is decreased in squamous cell carcinoma (SCC). The oncogenic role of MYC in the development of SCC is unclear since studies have shown in normal skin that wild-type MYC overexpression can drive loss of stem cells and epidermal differentiation. To investigate whether and how altered MYC phosphorylation might affect SCC development, progression, and metastasis, we generated mice with inducible expression of MYCWT or MYCT58A in the basal layer of the skin epidermis. In the T58A mutant, MYC is stabilized with constitutive S62 phosphorylation. When challenged with DMBA/TPA-mediated carcinogenesis, MYCT58A mice had accelerated development of papillomas, increased conversion to malignant lesions, and increased metastasis as compared to MYCWT mice. In addition, MYCT58A-driven SCC displayed stem cell gene expression not observed with MYCWT, including increased expression of Lgr6, Sox2, and CD34. In support of MYCT58A enhancing stem cell phenotypes, its expression was associated with an increased number of BrdU long-term label-retaining cells, increased CD34 expression in hair follicles, and increased colony formation from neonatal keratinocytes. Together, these results indicate that altering MYC phosphorylation changes its oncogenic activity—instead of diminishing establishment and/or maintenance of epidermal stem cell populations like wild-type MYC, pS62-MYC enhances these populations and, under carcinogenic conditions, pS62-MYC expression results in aggressive tumor phenotypes.
Exosome-transferred LINC01559 promotes the progression of gastric cancer via PI3K/AKT signaling pathway
Authors: Wang Liyan , Bo Xiaotong , Yi Xiaoyuan , Xiao Xuhua , Zheng Qinghua , Ma Lei , Li Bin 
Published: September 2020

AbstractIncreasing evidence indicates that long non-coding RNAs (lncRNAs) are associated with the progression of human cancers. However, the expression level and function of LINC01559 (long intergenic non-protein coding RNA 1559) in gastric cancer (GC) are rarely reported. Here we found that LINC01559 was upregulated in GC tissues based on GEPIA (Gene Expression Profiling Interactive Analysis) and TCGA (The Cancer Genome Atlas) databases. Also, LINC01559 was expressed at a lower level in GC cells than in mesenchymal stem cells (MSCs). In vitro experiments revealed that silencing LINC01559 remarkably hindered GC cell proliferation, migration and stemness. Then, we identified that LINC01559 was transmitted form MSCs to GC cells via the exosomes. Immunofluorescence staining and electron microscope validated the existence of exosomes in GC cells. Mechanistically, LINC01559 sponged miR-1343-3p to upregulate PGK1 (phosphoglycerate kinase 1), therefore activating PI3K/AKT pathway. Moreover, LINC01559 recruited EZH2 (enhancer of zeste 2 polycomb repressive complex 2 subunit) to PTEN (phosphatase and tensin homolog) promoter, inducing the methylation of PTEN promoter and finally resulting in PTEN repression. Of note, LINC01559 targeted both PGK1 and PTEN to promote GC progression by activating PI3K/AKT pathway. Taken together, our study demonstrated that LINC01559 accelerated GC progression via upregulating PGK1 and downregulating PTEN to trigger phosphatidylinositol 3-kinase/AKT serine/threonine kinase (PI3K/AKT) pathway, indicating LINC01559 as a potential biomarker for GC treatment.
Flavin dependency undermines proteome stability, lipid metabolism and cellular proliferation during vitamin B2 deficiency
Authors: Martínez-Limón Adrían , Calloni Giulia , Ernst Robert , Vabulas R. Martin 
Published: September 2020

AbstractTumor cells adapt their metabolism to meet the energetic and anabolic requirements of high proliferation and invasiveness. The metabolic addiction has motivated the development of therapies directed at individual biochemical nodes. However, currently there are few possibilities to target multiple enzymes in tumors simultaneously. Flavin-containing enzymes, ca. 100 proteins in humans, execute key biotransformations in mammalian cells. To expose metabolic addiction, we inactivated a substantial fraction of the flavoproteome in melanoma cells by restricting the supply of the FMN and FAD precursor riboflavin, the vitamin B2. Vitamin B2 deficiency affected stability of many polypeptides and thus resembled the chaperone HSP90 inhibition, the paradigmatic multiple-target approach. In support of this analogy, flavin-depleted proteins increasingly associated with a number of proteostasis network components, as identified by the mass spectrometry analysis of the FAD-free NQO1 aggregates. Proteome-wide analysis of the riboflavin-starved cells revealed a profound inactivation of the mevalonate pathway of cholesterol synthesis, which underlines the manifold cellular vulnerability created by the flavoproteome inactivation. Cell cycle-arrested tumor cells became highly sensitive to alkylating chemotherapy. Our data suggest that the flavoproteome is well suited to design synthetic lethality protocols combining proteostasis manipulation and metabolic reprogramming.
Mutual modulation between surface chemistry and bulk microstructure within secondary particles of nickel-rich layered oxides
Authors: Li Shaofeng , Jiang Zhisen , Han Jiaxiu , Xu Zhengrui , Wang Chenxu , Huang Hai , Yu Chang , Lee Sang-Jun , Pianetta Piero , Ohldag Hendrik , Qiu Jieshan , Lee Jun-Sik , Lin Feng , Zhao Kejie , Liu Yijin 
Published: September 2020

AbstractSurface lattice reconstruction is commonly observed in nickel-rich layered oxide battery cathode materials, causing unsatisfactory high-voltage cycling performance. However, the interplay of the surface chemistry and the bulk microstructure remains largely unexplored due to the intrinsic structural complexity and the lack of integrated diagnostic tools for a thorough investigation at complementary length scales. Herein, by combining nano-resolution X-ray probes in both soft and hard X-ray regimes, we demonstrate correlative surface chemical mapping and bulk microstructure imaging over a single charged LiNi0.8Mn0.1Co0.1O2 (NMC811) secondary particle. We reveal that the sub-particle regions with more micro cracks are associated with more severe surface degradation. A mechanism of mutual modulation between the surface chemistry and the bulk microstructure is formulated based on our experimental observations and finite element modeling. Such a surface-to-bulk reaction coupling effect is fundamentally important for the design of the next generation battery cathode materials.
A stabilized glycomimetic conjugate vaccine inducing protective antibodies against Neisseria meningitidis serogroup A
Authors: Enotarpi Jacopo , Tontini Marta , Balocchi Cristiana , van der Es Daan , Auberger Ludovic , Balducci Evita , Carboni Filippo , Proietti Daniela , Casini Daniele , Filippov Dmitri V. , Overkleeft Hermen S. , van der Marel Gijsbert A. , Colombo Cinzia , Romano Maria Rosaria , Berti Francesco , Costantino Paolo , Codeé Jeroen D. C. , Lay Luigi , Adamo Roberto 
Published: September 2020

AbstractNeisseria meningitidis serogroup A capsular polysaccharide (MenA CPS) consists of (1 → 6)-2-acetamido-2-deoxy-α-D-mannopyranosyl phosphate repeating units, O-acetylated at position C3 or C4. Glycomimetics appear attractive to overcome the CPS intrinsic lability in physiological media, due to cleavage of the phosphodiester bridge, and to develop a stable vaccine with longer shelf life in liquid formulation. Here, we generate a series of non-acetylated carbaMenA oligomers which are proven more stable than the CPS. An octamer (DP8) inhibits the binding of a MenA specific bactericidal mAb and polyclonal serum to the CPS, and is selected for further in vivo testing. However, its CRM197 conjugate raises murine antibodies towards the non-acetylated CPS backbone, but not the natural acetylated form. Accordingly, random O-acetylation of the DP8 is performed, resulting in a structure (Ac-carbaMenA) showing improved inhibition of anti-MenA CPS antibody binding and, after conjugation to CRM197, eliciting anti-MenA protective murine antibodies, comparably to the vaccine benchmark.
Integrin-alpha-6+ Candidate stem cells are responsible for whole body regeneration in the invertebrate chordate Botrylloides diegensis
Authors: Kassmer Susannah H. , Langenbacher Adam D. , De Tomaso Anthony W. 
Published: September 2020

AbstractColonial ascidians are the only chordates able to undergo whole body regeneration (WBR), during which entire new bodies can be regenerated from small fragments of blood vessels. Here, we show that during the early stages of WBR in Botrylloides diegensis, proliferation occurs only in small, blood-borne cells that express integrin-alpha-6 (IA6), pou3 and vasa. WBR cannot proceed when proliferating IA6+ cells are ablated with Mitomycin C, and injection of a single IA6+ Candidate stem cell can rescue WBR after ablation. Lineage tracing using EdU-labeling demonstrates that donor-derived IA6+ Candidate stem cells directly give rise to regenerating tissues. Inhibitors of either Notch or canonical Wnt signaling block WBR and reduce proliferation of IA6+ Candidate stem cells, indicating that these two pathways regulate their activation. In conclusion, we show that IA6+ Candidate stem cells are responsible for whole body regeneration and give rise to regenerating tissues.
Cyclin E expression is associated with high levels of replication stress in triple-negative breast cancer
Authors: Guerrero Llobet Sergi , van der Vegt Bert , Jongeneel Evelien , Bense Rico D. , Zwager Mieke C. , Schröder Carolien P. , Everts Marieke , Fehrmann Rudolf S. N. , de Bock Geertruida H. , van Vugt Marcel A. T. M. 
Published: September 2020

AbstractReplication stress entails the improper progression of DNA replication. In cancer cells, including breast cancer cells, an important cause of replication stress is oncogene activation. Importantly, tumors with high levels of replication stress may have different clinical behavior, and high levels of replication stress appear to be a vulnerability of cancer cells, which may be therapeutically targeted by novel molecularly targeted agents. Unfortunately, data on replication stress is largely based on experimental models. Further investigation of replication stress in clinical samples is required to optimally implement novel therapeutics. To uncover the relation between oncogene expression, replication stress, and clinical features of breast cancer subgroups, we immunohistochemically analyzed the expression of a panel of oncogenes (Cyclin E, c-Myc, and Cdc25A,) and markers of replication stress (phospho-Ser33-RPA32 and γ-H2AX) in breast tumor tissues prior to treatment (n = 384). Triple-negative breast cancers (TNBCs) exhibited the highest levels of phospho-Ser33-RPA32 (P < 0.001 for all tests) and γ-H2AX (P < 0.05 for all tests). Moreover, expression levels of Cyclin E (P < 0.001 for all tests) and c-Myc (P < 0.001 for all tests) were highest in TNBCs. Expression of Cyclin E positively correlated with phospho-RPA32 (Spearman correlation r = 0.37, P < 0.001) and γ-H2AX (Spearman correlation r = 0.63, P < 0.001). Combined, these data indicate that, among breast cancers, replication stress is predominantly observed in TNBCs, and is associated with expression levels of Cyclin E. These results indicate that Cyclin E overexpression may be used as a biomarker for patient selection in the clinical evaluation of drugs that target the DNA replication stress response.
International consensus recommendations on the diagnostic work-up for malformations of cortical development
Authors: Oegema Renske , Barakat Tahsin Stefan , Wilke Martina , Stouffs Katrien , Amrom Dina , Aronica Eleonora , Bahi-Buisson Nadia , Conti Valerio , Fry Andrew E. , Geis Tobias , Andres David Gomez , Parrini Elena , Pogledic Ivana , Said Edith , Soler Doriette , Valor Luis M. , Zaki Maha S. , Mirzaa Ghayda , Dobyns William B. , Reiner Orly , Guerrini Renzo , Pilz Daniela T. , Hehr Ute , Leventer Richard J. , Jansen Anna C. , Mancini Grazia M. S. , Di Donato Nataliya 
Published: September 2020

AbstractMalformations of cortical development (MCDs) are neurodevelopmental disorders that result from abnormal development of the cerebral cortex in utero. MCDs place a substantial burden on affected individuals, their families and societies worldwide, as these individuals can experience lifelong drug-resistant epilepsy, cerebral palsy, feeding difficulties, intellectual disability and other neurological and behavioural anomalies. The diagnostic pathway for MCDs is complex owing to wide variations in presentation and aetiology, thereby hampering timely and adequate management. In this article, the international MCD network Neuro-MIG provides consensus recommendations to aid both expert and non-expert clinicians in the diagnostic work-up of MCDs with the aim of improving patient management worldwide. We reviewed the literature on clinical presentation, aetiology and diagnostic approaches for the main MCD subtypes and collected data on current practices and recommendations from clinicians and diagnostic laboratories within Neuro-MIG. We reached consensus by 42 professionals from 20 countries, using expert discussions and a Delphi consensus process. We present a diagnostic workflow that can be applied to any individual with MCD and a comprehensive list of MCD-related genes with their associated phenotypes. The workflow is designed to maximize the diagnostic yield and increase the number of patients receiving personalized care and counselling on prognosis and recurrence risk.
Development of a fast and user-friendly cryopreservation protocol for sweet potato genetic resources
Authors: Wilms Hannes , Fanega Sleziak Natalia , Van der Auweraer Maarten , Brands Martijn , Verleije Matthijs , Hardeman Dirk , Andre Edwige , Panis Bart 
Published: September 2020

AbstractSweet potato (Ipomoea batatas) is one of the ten most important staple crops and provides a livelihood for many people around the globe. To adapt to ever-changing circumstances farmers and breeders need to have access to a broad diversity of germplasm. This study focuses on the development of a cryopreservation protocol that allows the long term storage of different sweet potato cultivars. For this, a droplet vitrification protocol was optimized, comparing several parameters; preculture method (0.3 M sucrose vs no preculture); meristem position (axillary vs apical); plant age (3 to 9 weeks); regeneration medium (MS + 2.22 µM BA, Hirai and MS); and length of loading solution treatment (20 to 360 min). Two months after cryopreservation, the regeneration rates of the meristems were compared, which resulted in significant differences for the preculture method, meristem position and loading solution. With these new insights an optimized droplet vitrification protocol was developed with the following parameters: use of 3–9 week old axillary meristems, no preculture phase, 20 min LS treatment, 30 min PVS2 treatment, exposure to liquid nitrogen by droplet vitrification, warming treatment in RS for 15 min, 1 day 0.3 M sucrose recuperation culture, 1 month MS + 2.22 µM BA followed by 1 month of MS cultures. This protocol was subsequently tested on 10 representative accessions resulting in a post cryopreservation regeneration rate of more than 40% for 70% of the tested cultivars, showing that this protocol could be implemented for a large portion of existing sweet potato collections.
Assessment of predictive validity and feasibility of Edmonton Frail Scale in identifying postoperative complications among elderly patients: a prospective observational study
Authors: He Yingke , Li Lydia Weiling , Hao Ying , Sim Eileen Yilin , Ng Kai Lee , Lee Rui , Lim Mattheaus ShengJie , Poopalalingam Ruban , Abdullah Hairil Rizal 
Published: September 2020

AbstractFrailty is defined as diminished physiological reserve predisposing one to adverse outcomes when exposed to stressors. Currently, there is no standardized Frail assessment tool used perioperatively. Edmonton Frail Scale (EFS), which is validated for use by non-geriatricians and in selected surgical populations, is a candidate for this role. However, little evaluation of its use has been carried out in the Asian populations so far. This is a prospective observational study done among patients aged 70 years and above attended Preoperative Assessment Clinic (PAC) in Singapore General Hospital prior to major abdominal surgery from December 2017 to September 2018. The Comprehensive Complication Index (CCI) and Postoperative Morbidity Survey (POMS) were used to assess their postoperative morbidity respectively. Patient’s acceptability of EFS was measured using the QQ-10 questionnaire and the inter-rater reliability of EFS was assessed by Kappa statistics and Bland Altman plot. The primary aim of this study is to assess if frailty measured by EFS is predictive of postoperative complications in elderly patients undergoing elective major abdominal surgery. We also aim to assess the feasibility of implementing EFS as a standard tool in the outpatient preoperative assessment clinic setting. EFS score was found to be a significant predictor of postoperative morbidity. (OR 1.35, p < 0.001) Each point increase in EFS score was associated with a 3 point increase in CCI score. (Coefficient b 2.944, p < 0.001) EFS score more than 4 has a fair predictability of both early and 30-day postoperative complications. Feasibility study demonstrated an overall acceptance of the EFS among our patients with good inter-rater agreement.