bluuebunny/crossref_metadata_2025_split huggingface dataset:
MicroRNA-325 targets lipocalin 15 to suppress proliferation, migration and invasion of breast cancer cells
Authors: Liu Zhuangkai , Xu Hong , Li Xiang , Zhang Ruishan , Bai Jinghui , Zhang Xinfeng 
Published: February 2020

IntroductionRecent studies have proved the diverse roles of miRs in different cancer-related processes. This study was undertaken to determine the therapeutic implications of miR-325-3p in breast cancer.Material and methodsExpression analysis was carried out by qRT-PCR. Transfections were performed by Lipofectamine 2000 reagent. MTT assay was used for cell viability. Transwell assays were used for cell migration and invasion. Western blot analysis was used for protein expression analysis.ResultsGene expression analysis revealed miR-325 to be significantly suppressed in breast cancer tissues and cell lines. Nonetheless, ectopic expression of miR-325 resulted in suppression of the growth and colony development potential of the SK-BR-3 and CAMA-1 cells. Transwell assays showed that miR-325 overexpression also resulted in the decline of the migration and invasion of the SK-BR-3 and CAMA-1 cells. Bioinformatic analysis showed that miR-325 targets lipocalin 15 (LNC15) in breast cancer cells. LNC15 was also overexpressed in the breast cancer tissues and cell lines. However, overexpression of miR-325 caused a significant decline in the LNC15 expression in SK-BR-3 cells. Additionally, silencing of LNC15 resulted in inhibition of the growth, migration and invasion of the SK-BR-3 cells. Rescue assay showed that overexpression of LNC15 could promote the growth, migration and invasion of the miR-325 overexpressing effects.ConclusionsTaken together, the evidence shows that miR-325 acts as a tumor suppressor in breast cancer and may be used in the treatment of breast cancer.
The impact on health-related quality of life of mixed mental and physical multimorbidity in adults aged 60 years and older: secondary analysis of primary care data
Authors: Prazeres Filipe , Santiago Luiz , Simões José 
Published: February 2020

IntroductionGiven the number of patients with mental conditions who receive treatment within the primary care (PC) context, and the high prevalence of multimorbidity (especially in older people), there is a need to study mental-physical multimorbidity (MPM) in this population and context. This study sought to identify the impact on health-related quality of life (QoL) of MPM in adults aged 60 years and older.Material and methodsSecondary analysis of data derived from 251 primary health individuals. Data were collected via a sociodemographic and clinical questionnaire. Health-related QoL was assessed using the SF-12 instrument. Multiple linear regressions were performed for physical and mental health in MPM patients and in patients with physical-only multimorbidity.ResultsMean age of participants was 70.6 years; 57.8% were female. Quali¬ty of life was lower in MPM patients than in those with physical-only multimorbidity. Regarding MPM patients, female sex, 75 years and over, and low income were associated with worse physical health. Female sex was also associated with worse mental health.ConclusionsThis study contributes to the global knowledge of MPM in older people, illuminates health-related QoL differences among MPM and physical- only multimorbidity patients, and highlights the importance of non-modi-fiable characteristics associated with deterioration of health-related QoL. Team collaboration between primary care physicians, psychiatrists (and other mental health providers), and social workers may be necessary to assess psychiatric and physical symptoms and provide for the care needs of older people with MPM.
Twelve-month clinical results from the new cobalt-chromium sirolimus-eluting dedicated bifurcation stent BiOSS LIM C Registry
Authors: Gil Robert J. , Kern Adam , Pawłowski Tomasz , Bil Jacek 
Published: February 2020

IntroductionPercutaneous coronary interventions (PCI) in bifurcations are still challenging and are associated with higher risks of periprocedural complications as well as restenosis and stent thrombosis. The aim of this paper was to summarize 12 months of clinical results of the prospective, first-in-man registry assessing the BiOSS LIM C stent (Balton, Poland).Material and methodsIn the prospective two-center registry we enrolled patients with non-ST-segment elevation acute coronary syndrome (NSTE-ACS) and stable coronary artery disease. Provisional T-stenting was the default treatment strategy. The primary endpoint was defined as the rate of cardiac death, myocardial infarction (MI) and clinically driven target lesion revascularization (TLR) in 12-month follow-up.ResultsThe study population consisted of 95 patients (mean age: 66.8 ±9.8 years, 17.9% were females). A BiOSS LIM C stent was implanted in the left main (LM) in 53 (55.8%) cases. There were 25.2% of patients with NSTE-ACS, 33.7% with diabetes, 90.5% with hypertension, and 53.7% had previous MI. The device success rate was 100%. An additional regular drug-eluting stent was deployed in the side branch in 18.9% of cases. Proximal optimization technique and final kissing balloon (FKB) technique were used in 53.7% and 30.5% of cases, respectively. MI type 4a was registered in 4 (4.2%) cases. At 12 months the MACE rate was 9.5%, cardiac death 1.1%, MI 2.1% and clinically driven TLR 6.3%. All incidents, apart from one TLR, appeared in the LM subgroup.ConclusionsOur registry might suggest that PCI using the BiOSS LIM C in coronary bifurcations is feasible and might be an option for percutaneous revascularization.
Enoxolone suppresses apoptosis in chondrocytes and progression of osteoarthritis via modulating the ERK1/2 signaling pathway
Authors: Hong Gang 
Published: February 2020

IntroductionOsteoarthritis (OA) is an inflammatory disorder of synovial joints which is mainly treated with therapeutic agents showing side effects associated with the gastrointestinal (GI) and metabolic system. Consequently, there is urgent need for a potent, safe and novel agent for treating OA and related disorders. Enoxolone is a pentacyclic triterpenoid obtained from the herb liquorice. Based on earlier findings, we postulated that enoxolone may produce chondroprotective activity by exerting anti-inflammatory, anti-catabolic and oxidative stress-decreasing effects.Material and methodsThe chondrocytes were extracted from the femoral head articular cartilage of healthy rats. Immunofluorescence staining was done for identification of chondrocytes. Cell viability and proliferation studies were done using Cell Counting Kit-8. Apoptotic cells were identified by TUNEL assay and flow cytometry analysis. Autophagy was assessed by monodansylcadaverine assay. Western blot analysis was done for expression of proteins.ResultsIn the present study we investigated the protective effect of enoxolone on interleukin 1β (IL-1β) treated Iry chondrocytes in vitro. Treatment with IL-1β resulted in a significant reduction in cell viability of cells in increasing dose and time. Treatment with enoxolone along with IL-1β caused a significant decrease in growth inhibition. Also, enoxolone inhibited the IL-1β mediated apoptosis and activation of caspase-3 in cells. We also observed that enoxolone elevated the levels of p-ERK1/2, light chain 3 (LC3)-II and Beclin-1 (autophagy markers) in chondrocytes. The expression of (LC3)-II and Beclin-1 was decreased when the cells were treated with U0126 (ERK1/2 inhibitor).ConclusionsOur findings demonstrate that enoxolone could suppress inflammatory signaling and apoptosis via the ERK1/2 pathway in chondrocytes.
mRNA-103 inhibition attenuates autophagy and inflammation in myocardial infarction by regulating the TLR4 pathway
Authors: Li Fan , Ma XiaoHui , Liu Tong , Wang Yang , Xie Xin , Xin Yi , Cheng Kang 
Published: February 2020

IntroductionThis investigation determined the cardioprotective activity of mRNA-103 inhibitor against myocardial infarction (MI) and also evaluated its molecular mechanism.Material and methodsMI was induced in rats by inducing myocardial ischaemia/reperfusion (I/R), and left ventricular (LV) mRNA-103 (1 × 10<sup>7</sup> TU) was injected into the myocardium around the infarcted area. The effect of mRNA-103 inhibitor was assessed by determining the levels of myocardial enzymes and cytokines in the serum, the myeloperoxidase (MPO) activity, and the levels of Toll-like receptor 4 (TLR4), nuclear factor κ-light-chain enhancer of activated B cells (NF-κB), and MyD88 mRNAs in the myocardial tissues of MI rats. Immunocytochemical analysis and a histopathology study were also performed.ResultsThe levels of myocardial enzymes and cytokines were lower in the mRNA-103 inhibitor-treated group than in the group in which the only treatment was the induction of MI. There was a lower percentage of infarcted area and a lower apoptosis index in the mRNA-103 inhibitor-treated group compared to the MI-only. The levels of TLR4, NF-B, and MyD88 mRNAs were lower in the myocardial tissues of the mRNA-103 inhibitor-treated group than in the MI-only group. Immunohistochemical analysis revealed that treatment with mRNA-103 inhibitor ameliorated the expression of TLR4 in the myocardial tissues of MI rats.ConclusionsThe data revealed that inhibition by mRNA-103 protects against myocardial injury in MI rats by regulating the inflammasome pathway.
Neuroprotective Potentials of Natural Vitamin E for Cerebral Small Vessel Disease
Authors: Mustapha Muzaimi , Mohd Nasril Che Mohd Nassir Che , Kah Hay Yuen , Wai Yee Fung , Abdul Hamid Hafizah 
Published: February 2020

Cerebral small vessel disease (CSVD) refers to a spectrum of clinical and neuroimaging findings resulting from pathological processes of various etiologies affecting cerebral arterioles, perforating arteries, capillaries, and venules. It is the commonest neurological problem that results in significant disability, but awareness of it remains poor. It affects over half of people over 65 years old and inflicts up to third of acute strokes, over 40% of dementia, and a significant decline in physical ability in otherwise asymptomatic, aging individuals. Moreover, the unifying theory for the pathomechanism of the disease remains elusive and hence the apparent ineffective therapeutic approaches. Given the growing literature for natural vitamin E (tocopherols and tocotrienols) as a potent antioxidant, this chapter attempts to consolidate the contemporary evidence to shed plausible insights on the neuroprotective potentials of natural vitamin E in addressing the heterogenous CSVD spectrum, in health and in disease.
Validación del Approaches and Study Skills Inventory for Students (ASSIST) en Universitarios de Buenos Aires, Argentina [Approaches and Study Skills Inventory for Students (ASSIST). Its validation for undergraduates from Buenos Aires, Argentina]
Authors: Freiberg Hoffmann Agustín , Romero Medina Agustín 
Published: February 2020

ResumenLos enfoques de aprendizaje permiten conocer las preferencias que manifiestan las personas para enfrentar una tarea de aprendizaje específica. Una de las herramientas más difundidas para evaluar los enfoques de aprendizaje es el Approaches and Study Skills Inventory for Students (ASSIST), que en su versión abreviada permite valorar mediante 18 ítems tres enfoques de aprendizaje (Profundo, Superficial, Estratégico). Dada la importancia que este instrumento tiene para evaluar los enfoques de aprendizaje en estudiantes universitarios, el objetivo de esta investigación es validar la versión española para el contexto argentino. Para ello se adecuó primero lingüísticamente el inventario a los modismos locales, para luego examinar evidencias de validez de constructo (análisis factorial exploratorio, confirmatorio, e invarianza factorial) y evidencias de validez concurrente. También se estudió la confiabilidad del instrumento mediante el análisis de consistencia interna. Se obtuvo una herramienta compuesta de 14 reactivos que posibilita evaluar los enfoques de aprendizaje Profundo, Superficial y Estratégico propuestos por la teoría. El modelo resultante de los procedimientos exploratorio y confirmatorio exhibió una adecuada invarianza factorial, y las dimensiones del inventario presentaron índices de consistencia interna superiores a .80. Además se obtuvieron evidencias de validez concurrente coherentes con la teoría y con resultados empíricos previos. AbstractThe study of approaches to learning enables the descrip­tion of preferences manifested when facing a specific learning task. One of the most widespread instruments for assessing learning approaches is the Approaches and Study Skills Inventory for Students (ASSIST). In its short version of 18 items, the ASSIST measures three learning approaches (Deep, Surface and Strategic). Given that the availability of this instrument would be of great value and needed to research learning approaches in college stu­dents, the objective of this research was to develop an Ar­gentinean version. To meet this end, the inventory under­went an initial linguistic adaptation to the local forms of expression and idioms. Then, construct validity was ex­amined (exploratory and confirmatory factorial analysis, and factorial invariance) together with analyses of concur­rent validity. Reliability was examined by means of anal­yses of internal consistency. The resulting 14-item instru­ment may be used to describe approaches to learning as proposed by the theory: Deep, Superficial and Strategic. The measure obtained by exploratory and confirmatory procedures showed an adequate factorial invariance. The dimensions of the inventory had internal consistency in­dexes greater than .80. Finally, evidences of concurrent validity were coherent with the theory and with previous empirical results.
Validation of the Morale Questionnaire for Military Operational Theaters [Validación del cuestionario de moral para zonas de Operaciones Militares]
Authors: Pastor Álvarez Alberto , Molero Alonso Fernando , Moriano León Juan Antonio 
Published: February 2020

AbstractTo assess the morale of the troops has been a concern of military leaders throughout history, mainly because of the level of involvement that this factor could have in the resolution of conflicts on the battlefield. The purpose of this research was to obtain evidence of the validity of the internal structure of the Morale Questionnaire used by the Spanish Armed Forces in military operations overseas. Two subsamples of 250 Spanish soldiers deployed on an international mission in Lebanon participated in the study. The questionnaire was applied at the beginning and at the end of the mission, respectly. The results obtained throughExploratory and Confirmatory Factorial Analysis and Structural Equation Modelling, allowed to assess the questionnaire statistically obtaining a tool that consists of 26 items, agglutinated in six factors. The theoretical dimensions of the original tool were mostly maintained. This will permit Spanish Armed Forces to have a reliable measuring instrument that will facilitate specific predictions about morale and its consecuences on the battlefield.ResumenEvaluar la moral de las tropas ha sido una preocupación de los líderes militares a lo largo de la historia, principalmente por el nivel de participación que este factor podría tener en la resolución de conflictos en el campo de batalla. El objetivo de esta investigación fue obtener evidencias sobre la validez de la estructura interna del cuestionario de moral utilizado por las Fuerzas Armadas Españolas en operaciones militares en el exterior. Dos submuestras de 250 soldados españoles desplegados en una misión internacional en Líbano participaron en el estudio. El cuestionario se aplicó al principio y al final de la misión. Los resultados obtenidos a través del Análisis factorial exploratorio y confirmatorio, así como el Modelo de ecuaciones estructurales, permitieron validar el cuestionario obteniendo estadísticamente una herramienta que consta de 26 ítems, aglutinados en seis factores. Las dimensiones teóricas de la herramienta original se mantuvieron en su mayoría. Esto permitirá a las Fuerzas Armadas españolas tener un instrumento de medición confiable que facilitará predicciones específicas sobre la moral y sus consecuencias en el campo de batalla.
Crewing of Sea Vessels Taking into Account Project Risks and Technical Condition of Ship Equipment
Authors: Zakharchenko Oleg V. , Bedrii Dmytro I. , Bileha Olena V. , Savina Oksana Y. , Haidaienko Oksana V. 
Published: February 2020

Motivation: One of the main concepts in project management is the concept of â€œteamâ€ in the project, and in project management - the human resources management of the project, which includes the processes of planning, forming and creating a team, its development and support activities, transformation or disbandment of the team. Despite the great attention paid to the formation of project management teams, existing studies do not fully highlight the specifics and features of crew operations. Criteria for the quantitative optimization of the ship's crew should be consistent with the main objectives of the project.Novelty: The research paper proposes an approach that allows optimizing the quantitative composition of the crew of a ship by more accurately assessing the level of project risks and costs associated with the maintenance of ship equipment. The practical application of this approach will optimize the quantitative composition of the ship's crew, which will both satisfy the needs of managing the technical equipment and minimize the risks and costs of the shipowner.Methodology and Methods: Risk management tools were used to achieve the objective and test the hypotheses suggested in the research, namely: methodology for estimating the net present value of the project; the method of estimating internal rate of return for the project; the method of estimating the return on investment in the project; the method of estimation for the period of return on investment costs in the project; the method of estimating the discounted payback period for the project, as well as the tools of simulation modellingÂ (Monte Carlo simulation method). The method of identification and grouping in the process of classification of project risks in the sphere of marine transportation, methods of systematization, grouping and logical generalization were also applied for systematization of information, drawing conclusions and making scientific suggestions in the research.Policy Considerations: Shipping plays an important role in the trade and tourism industry; human factor is the most important aspect that determines the efficiency of shipping development; maintaining of technical and technological processes of the ship puts certain requirements to the quantitative and qualitative composition of the team, deviation from which leads to the occurrence of certain risk events; formation of an effective model of ship's crew manning is the main link in ensuring effective shipping project management.
A comparative study of machine learning algorithms for predicting acute kidney injury after liver cancer resection
Authors: Lei Lei , Wang Ying , Xue Qiong , Tong Jianhua , Zhou Cheng-Mao , Yang Jian-Jun 
Published: February 2020

Objective
Machine learning methods may have better or comparable predictive ability than traditional analysis. We explore machine learning methods to predict the likelihood of acute kidney injury after liver cancer resection.

Methods
This is a secondary analysis cohort study. We reviewed data from patients who had undergone resection of primary hepatocellular carcinoma between January 2008 and October 2015.

Results
The analysis included 1,173 hepatectomy patients, 77 (6.6%) of whom had AKI and 1,096 (93.4%) who did not. The importance matrix for the Gbdt algorithm model shows that age, cholesterol, tumor size, surgery duration and PLT were the five most important parameters. Figure 1 shows that Age, tumor size and surgery duration had weak positive correlations with AKI. Cholesterol and PLT also had weak negative correlations with AKI. The models constructed by the four machine learning algorithms in the training group were compared. Among the four machine learning algorithms, random forest and gbm had the highest accuracy, 0.989 and 0.970 respectively. The precision of four of the five algorithms was 1, random forest being the exception. Among the test group, gbm had the highest accuracy (0.932). Random forest and gbm had the highest precision, both being 0.333. The AUC values for the four algorithms were: Gbdt (0.772), gbm (0.725), forest (0.662) and DecisionTree (0.628).

Conclusions
Machine learning technology can predict acute kidney injury after hepatectomy. Age, cholesterol, tumor size, surgery duration and PLT influence the likelihood and development of postoperative acute kidney injury.
Novel association between TGFA, TGFB1, IRF1, PTGS2 and IKBKB single-nucleotide polymorphisms and occurrence, severity and treatment response of major depressive disorder
Authors: Bialek Katarzyna , Czarny Piotr , Watala Cezary , Wigner Paulina , Talarowska Monika , Galecki Piotr , Szemraj Janusz , Sliwinski Tomasz 
Published: February 2020

BackgroundActivation of the immune system might affect the severity of depressive episodes as well as response to the antidepressant treatment. The purpose of this study was to investigate whether the occurrence of variant alleles of analyzed SNPs are involved in prevalence and progression of depression. Moreover, selected genes and SNPs have not been investigated in context of the disease severity and treatment. Therefore, six polymorphisms were selected: g.41354391A>G-TGFB1(rs1800469), g.132484229C>A-IRF(rs2070729), g.186643058A>G-PTGS2(rs5275), g.186640617C>T-PTGS2(rs4648308), g.70677994G>A-TGFA(rs2166975) and g.42140549G>T–IKBKB(rs5029748).MethodsA total of 360 (180 patients and 180 controls) DNA samples were genotyped using TaqMan probes.ResultsWe observed that A/G of the rs2166975TGFA, A/C of rs2070729IRF1and G/T of rs5029748IKBKBwere associated with an increased risk of depression development while the T/T of rs5029748IKBKB, T/T of rs4648308PTGS2and G/G of rs2166975TGFAreduced this risk. We also stratified the study group according to gender and found that genotype A/G and allele G of the rs2166975TGFA, G/T of rs5029748IKBKBas well as C allele of rs4648308PTGS2, homozygote A/A and allele A of rs5275PTGS2were associated with increased risk of depression development in men while homozygote G/G of rs5275PTGS2decreased this risk. Moreover, C/T of rs4648308PTGS2and A/G of rs5275PTGS2was positively correlated with the risk of the disease occurrence in women. Furthermore, a gene–gene analysis revealed a link between studied polymorphisms and depression. In addition, A/A of rs1800469TGFB1was associated with earlier age of onset of the disease while G/G of this SNP increased severity of the depressive episode. Interestingly, A/C of rs2070729IRF1and T/T of rs5029748IKBKBmay modulate the effectiveness of selective serotonin reuptake inhibitors therapy. In conclusion, studied SNPs may modulate the risk of occurrence, age of onset, severity of the disease and response to the antidepressant treatment.