bluuebunny/crossref_metadata_2025_split huggingface dataset:
The future of carbon accounting research: “we’ve pissed mother nature off, big time”
Authors: Gibassier Delphine , Michelon Giovanna , Cartel Mélodie 
Published: March 2020

Purpose
The purpose of this paper is to review the contributions of the special issue papers while presenting four broad research avenues.

Design/methodology/approach
The paper is based on a review of current literature on climate change and carbon accounting.

Findings
The authors propose four broad avenues for research: climate change as a systemic and social issue, the multi-layered transition apparatus for climate change, climate vulnerability and the future of carbon accounting.

Practical implications
The authors connect this study with the requested institutional changes for climate breakdown, making the paper relevant for practice and policy. The authors notably point to education and professions as institutions that will request bold and urgent makeovers.

Social implications
The authors urge academics to reconsider climate change as a social issue, requiring to use new theoretical lenses such as emotions, eco-feminism, material politics and “dispositifs” to tackle this grand challenge.

Originality/value
This paper switches the authors’ viewpoint on carbon accounting to look at it from a more systemic and social lens.
Exendin‐4 protects the hearts of rats from ischaemia/reperfusion injury by boosting antioxidant levels and inhibition of JNK/p66Shc/NADPH axis
Authors: Eid Refaat A. , Zaki Mohamed Samir Ahmed , Alaa Eldeen Muhammad , Alshehri Majed M. , Shati Ayed A. , El‐kott Attalla Farag 
Published: March 2020

AbstractExendin‐4, a glucagon‐like peptide‐1 receptor agonist, was shown to protect against cardiac ischaemia/reperfusion (I/R) injury by suppressing oxidative stress. p66Shc, a pro‐oxidant and an apoptotic protein, is activated in the infarcted left ventricles (LVs) after induction of I/R. This study investigated if the cardiac protective effect of Exendin‐4 against I/R injury in rats involves inhibition of p66Shc and to determine the underlying mechanisms behind this. Adult male rats (n = 12/group) were divided into four groups as a sham, a sham + Exendin‐4, an I/R, and an I/R + Exendin‐4. Exendin‐4 was administered to rats 7 days before the induction of I/R. Ischaemia was induced by ligating the left anterior descending (LAD) coronary artery for 40 minutes followed by reperfusion for 10 minutes. The infarct myocardium was used for further analysis. Exendin‐4 significantly reduced infarct area (by 62%), preserved LV function and lowered serum levels of LDH and CK‐MB in I/R‐induced rats. Also, it significantly reduced LV levels of ROS and MDA and protein levels of cytochrome‐c and cleaved caspase‐3 but significantly increased levels of glutathione (GSH) and manganese superoxide dismutase (MnSOD) in LVs of I/R rats indicating antioxidant and anti‐apoptotic effects. Furthermore, it inhibited JNK and p66Shc activation and downregulated protein levels of p66Shc and NADPH oxidase with no effect on protein levels/activity of p53 and PKCβII. Of note, Exendin‐4 also increased GSH and MnSOD in LVs of control rats. In conclusion, Exendin‐4 cardioprotective effect in I/R hearts is mediated mainly by antioxidant effect and inhibition of JNK/P66Shc/NADPH oxidase.
Reduced store‐operated Ca2+ entry impairs mesenteric artery function in response to high external glucose in type 2 diabetic ZDF rats
Authors: Schach Christian , Wester Michael , Leibl Florian , Redel Andreas , Gruber Michael , Maier Lars S. , Endemann Dierk , Wagner Stefan 
Published: March 2020

AbstractDiabetes is a major risk factor for cardiovascular disease, affecting both endothelial and smooth muscle cells. Store‐operated Ca2+ channels (SOCCs) have been implicated in many diabetic complications. Vascular dysfunction is common in patients with diabetes, but the role of SOCCs in diabetic vasculopathy is still unclear. Our research aimed to investigate the effects of high glucose (HG) on store‐operated Ca2+ entry (SOCE) in small arteries. Small mesenteric arteries from type 2 diabetic Zucker fatty rats (ZDF) versus their non‐diabetic controls (Zucker lean, ZL) were examined in a pressurized myograph. Vascular smooth muscle cells (VSMC) were isolated and intracellular Ca2+ was measured (Fura 2‐AM). A specific protocol to deplete intracellular Ca2+ stores and thereby open SOCCs, as well as pharmacological SOCE inhibitors (SKF‐96365, BTP‐2), were used to artificially activate and inhibit SOCE, respectively. High glucose (40 mmol/L) relaxed arteries in a SKF‐sensitive manner. Diabetic arteries exhibited reduced HG‐induced relaxation, as well as reduced contraction after Ca2+ replenishment. Further, the rise in intracellular Ca2+ on account of SOCE is diminished in diabetic versus non‐diabetic VSMCs and was insensitive to HG in diabetic VSMCs. The expression of SOCC proteins was measured, detecting a downregulation of Orai1 in diabetes. In conclusion, diabetes leads to a reduction of SOCE and SOCE‐induced contraction, which is unresponsive to HG‐mediated inhibition. The reduced expression of Orai1 in diabetic arteries could account for the observed reduction in SOCE.
Quality evaluation of different varieties of Zanthoxylum bungeanum Maxim. peels based on phenolic profiles, bioactivity, and HPLC fingerprint
Authors: Yu Li , Wu Wenli , Pan Yiying , Wang Wei , Sun Leiwen , Liu Yi , Wang Dongmei , Li Dengwu 
Published: March 2020

AbstractThis work aims to evaluate the quality of seven varieties of Zanthoxylum bungeanum peels based on phenolic compounds, bioactivity, and HPLC fingerprint combined with chemometrics analysis, and pick out the key chemical compounds. As a result, S2 (Fengxian Dahongpao) showed the highest content of total phenolic and rutin, as well as the strongest antioxidant activity, followed by S6 (Hancheng Shizitou). From HPLC fingerprint, eight common characteristic peaks were selected and proved to be effective in controlling the quality of Z. bungeanum peels with the total contribution of 92.775%. The similarity values of each sample ranged between 0.696 and 0.970. In addition, hyperoside was identified as another key chemical indicator for quality evaluation of Z. bungeanum peels by PCA. Accordingly, seven varieties of Z. bungeanum were classified into four groups, among which group 4 (Fengxian Dahongpao, S2) was considered as an outstanding variety, followed by group 1 (S5, S6, and S7) and group 3 (S3 and S4), and group 2 (Fugu late‐maturing, S1) was the last one. These results will be helpful to establish an effective and comprehensive evaluation system of Z. bungeanum.Practical ApplicationThis study not only provides another index for quality evaluation of Z. bungeanum peels, but it also expects to be a theoretical basis for high‐quality germplasm cultivation and the development of Z. bungeanum peels as food additives, pharmaceutical, or health care products.
Long non‐coding RNA SNHG6 promotes the growth and invasion of non‐small cell lung cancer by downregulating miR‐101‐3p
Authors: Li Ke , Jiang Yongxin , Xiang Xudong , Gong Quan , Zhou Chunyan , Zhang Lijuan , Ma Qianli , Zhuang Li 
Published: March 2020

BackgroundThe aim of this study was to determine the function of long non‐coding RNA small nucleolar RNA host gene 6 (SNHG6) in non‐small cell lung cancer (NSCLC) and its underlying mechanisms.MethodsThe association of SNHG6 or miR‐101‐3p with clinicopathological characteristics and prognosis in patents with NSCLC was assessed by TCGA dataset. Cell proliferation and invasion were evaluated by MTT and Transwell assays and SNHG6‐specific binding with miR‐101‐3p was verified by bioinformatic analysis, luciferase gene report and RNA immunoprecipitation assays. qRT‐PCR and Western blot was used to assess the effects of SNHG6 on the expression of miR‐101‐3p and chromodomain Y like (CDYL) in NSCLC cells. A xenograft tumor model in vivo was established to observe the effects of SNHG6 knockdown on tumor growth.ResultsWe found that increased expression of SNHG6 was associated with pathological stage and lymph node infiltration, and acted as an independent prognostic factor of tumor recurrence in patients with NSCLC. Silencing SNHG6 expression repressed cell growth and invasion in vitro and in vivo, but overexpression of SNHG6 reversed these effects. Furthermore, SNHG6 was identified to act as a sponge of miR‐101‐3p, which could reduce cell proliferation and attenuate SNHG6‐induced CDYL expression. Low expression of miR‐101‐3p or high expression of CDYL was related to poor survival in patients with NSCLC.ConclusionsOur findings demonstrated that lncRNA SNHG6 contributed to the proliferation and invasion of NSCLC by downregulating miR‐101‐3p.
Effects of simple active referrals of different intensities on smoking abstinence and smoking cessation services attendance: a cluster‐randomized clinical trial
Authors: Weng Xue , Luk Tzu Tsun , Suen Yi Nam , Wu Yongda , Li Ho Cheung William , Cheung Yee Tak Derek , Kwong Antonio Cho Sshing , Lai Vienna Wai Yin , Chan Sophia Siu Chee , Lam Tai‐Hing , Wang Man Ping 
Published: March 2020

AbstractBackground and aimsProactive brief cessation advice by a lay counsellor combined with a referral to a smoking cessation service (active referral) is effective in increasing service use and quitting in community smokers. We compared the effect of two modified approaches to referrals on the cessation outcomes in community smokers.DesignThree‐arm cluster‐randomized trial.SettingGeneral community in Hong Kong.ParticipantsDaily cigarette smokers (n = 1163; 77.7% male).InterventionsParticipants were randomized to receive on‐site active referral (OSR, n = 395), where lay counsellors helped participants make appointments with a smoking cessation service of their choice plus tailored reminders; mobile text messaging referral (TMR, n = 385), where participants were encouraged to use a smoking cessation service via text messages; or brief cessation advice only (control, n = 383).MeasurementsThe primary outcome was a self‐reported 7‐day point‐prevalence abstinence at 6 months post‐treatment initiation. Secondary outcomes included 7‐day point‐prevalence abstinence at 3 and 18 months, biochemically validated abstinence, smoking reduction and the use of cessation services at 3, 6 and 18 months.FindingsUsing intention‐to‐treat analysis, the OSR (17.7%) and TMR (17.1%) groups had significantly higher self‐reported abstinence than the control (12.0%) group at 6 months [odds ratio (OR) for OSR versus control = 1.58, 95% confidence interval (CI) = 1.06–2.36; OR for TMR versus control = 1.52, 95% CI = 1.01–2.28; both P < 0.05]. The corresponding validated abstinence rates at 6 months were 7.6, 7.8 and 3.9% (OR for TMR versus control = 2.02, 95% CI = 1.07–3.81; OR for TMR versus control = 2.07, 95% CI = 1.10–3.92; both P < 0.05). Self‐reported and validated abstinence were similar at 18 months. OSR groups had higher rates of smoking cessation service use than the control group at all follow‐ups (all P < 0.001). The smoking reduction rates were similar in continuing smokers.ConclusionsSimple active referrals (in person or via text messaging) to smoking cessation services increased abstinence rates among smokers in Hong Kong compared with general brief cessation advice. On‐site active referral increased the use of smoking cessation services compared with general brief cessation advice.
From ‘neurotic’ to ‘managing’ mother: The ‘medical career’ experienced by mothers of a child diagnosed with Juvenile Idiopathic Arthritis
Authors: Waite‐Jones Jennifer M. , Swallow Veronica , Madill Anna 
Published: March 2020

ObjectiveDespite increased research into how caring for a child diagnosed with juvenile idiopathic arthritis (JIA) affects mothers, more needs to be known about ways in which such experiences transform their lives. Insight into the experiences of such mothers was sought through analysis of interviews with eight mothers and one father caring for a child with JIA.DesignThe study is situated within a larger project involving families with a child with JIA. A social constructionist approach was adopted and grounded theory including a ‘negative case,’ guided gathering and analysing data. Individual, semi‐structured interviews were conducted based on the research question: ‘What is it like to be the mother of a child with juvenile idiopathic arthritis?’ResultsFindings suggest that mothers find difficulty living up to the ‘ideal mother’ expected within Western society when forced to provide competing demands of age‐related, yet illness‐relevant care. The unpredictable nature of JIA means mothers face a lack of understanding from professionals so become hyper‐vigilant, ‘battling’ on behalf of their ill child. A self‐perpetuating loop develops if this is misperceived as being overprotective, leaving mothers vulnerable to being judged ‘neurotic’. However, with experience, often at an emotional cost, such mothers’ confidence in managing the competing demands of caring for their ill child increases such they can navigate a positive journey from ‘neurotic’ to ‘managing’ mother.ConclusionsUnderstanding this process could help health care professionals reduce stressful experiences faced by mothers when caring for a chronically ill child.
Statement of contribution

What is already known on this subject?

Mothers of chronically ill children are often diagnosed with anxiety and depression. Such mothers will have taken over managing their child's condition, and this can include negative experiences within the health care system. Qualitative methods enable deeper understanding of the experiences of mothers of chronically ill children.

What does the study add?

Potentially gendered position of mothers of children with chronic conditions.

Taken for granted assumptions implicitly underpin interactions between mothers and some professionals.

Greater partnership between mothers and professionals ultimately improves the care of the ill child.
How often are outcomes other than change in substance use measured? A systematic review of outcome measures in contemporary randomised controlled trials
Authors: Bjornestad Jone , McKay James R. , Berg Henrik , Moltu Christian , Nesvåg Sverre 
Published: March 2020

AbstractIssuesRecovery is a theoretical construct and empirical object of inquiry. The aim was to review whether outcome measures used in randomised controlled trials of drug treatment reflect a comprehensive conceptualisation of recovery.ApproachSystematic review using the following databases: Cochrane Database of Systematic Reviews, Cochrane Controlled Register of Trials, Database of Abstracts of Reviews of Effect, Web of Science, MEDLINE, Embase and PsycINFO. Search returned 6556 original articles and 504 met the following inclusion criteria: randomised controlled trial in English‐language peer‐reviewed journal; sample meets criteria for drug dependence or drug use disorder; reports non‐substance use treatment outcomes. Review protocol registration: PROSPERO (CRD42018090064).Key Findings3.8% of the included studies had a follow up of 2 years or more. Withdrawal/craving was present in 31.1% of short‐term versus 0% of long‐term studies. Social functioning in 8% of short‐term versus 36.8% of long‐term studies. Role functioning (0.9 vs. 26.3%), risk behaviour (15.6 vs. 36.8%) and criminality (3.8 vs. 21.1%) followed a similar pattern. Housing was not examined short‐term and unregularly long‐term (2.0%). ‘Use of health‐care facilities’, clinical psychological, behavioural factors were frequently reported. Physiological or somatic health (15.2 vs. 10.5%), motivation (14.2 vs. 15.8%) and quality of life (7.1 vs. 0%) were less frequently reported.ConclusionThe short time interval of the follow up and lack of information on relevant factors in recovery prevents the development of evidence‐based approaches to improve these factors. Particularly, measures of social and role functioning should be added to reflect an adequate conceptualisation of recovery.
Interaction of Bacillus subtilis GabR with the gabTD promoter: role of repeated sequences and effect of GABA in transcriptional activation
Authors: Nardella Caterina , Barile Anna , di Salvo Martino L. , Milano Teresa , Pascarella Stefano , Tramonti Angela , Contestabile Roberto 
Published: March 2020

Bacillus subtilis is able to use γ‐aminobutyric acid (GABA) found in the soil as carbon and nitrogen source, through the action of GABA aminotransferase (GabT) and succinic semialdehyde dehydrogenase (GabD). GABA acts as molecular effector in the transcriptional activation of the gabTD operon by GabR. GabR is the most studied member of the MocR family of prokaryotic pyridoxal 5′‐phosphate (PLP)‐dependent transcriptional regulators, yet crucial aspects of its mechanism of action are unknown. GabR binds to the gabTD promoter, but transcription is activated only when GABA is present. Here, we demonstrated, in contrast with what had been previously proposed, that three repeated nucleotide sequences in the promoter region, two direct repeats and one inverted repeat, are specifically recognized by GabR. We carried out in vitro and in vivo experiments using mutant forms of the gabTD promoter. Our results showed that GABA activates transcription by changing the modality of interaction between GabR and the recognized sequence repeats. A hypothetical model is proposed in which GabR exists in two alternative conformations that, respectively, prevent or promote transcription. According to this model, in the absence of GABA, GabR binds to DNA interacting with all three sequence repeats, overlapping the RNA polymerase binding site and therefore preventing transcription activation. On the other hand, when GABA binds to GabR, a conformational change of the protein leads to the release of the interaction with the inverted repeat, allowing transcription initiation by RNA polymerase.
Association between 5‐hydroxytryptamine gene polymorphism rs140700 and primary insomnia in Chinese population
Authors: Hui Peilin , Yang Jun , Wang Jing , Zhao Lijun , Wang Xubin , Su Xiaoyan , Wang Jinfeng , Ma Wei , Fan Jie , Chen Wenjuan , Zhao Yuan , Guo Bin , Liu Yu , Zhang Shangli , Zheng Ning , Zhou Liyan , Xie Yuping 
Published: March 2020

AbstractBackgroundPrimary insomnia is a worldwide problem and it has a considerable negative impact on one's physical and mental health. Studies have shown that non‐synonymous Single‐nucleotide polymorphisms in 5‐hydroxytryptamine (serotonin or 5‐HT) are related to primary insomnia. Previous studies have shown that 5‐HT polymorphism (rs140700) is related to depression, and insomnia is often accompanied by depression and anxiety. The relationship between this site and primary insomnia is unknown. We speculated that this site may be related to primary insomnia, so we investigated the relationship between rs140700 and primary insomnia.AimsTo explore the relationship between the 5‐HT gene polymorphism rs140700 and primary insomnia.MethodsIn this study, we included 57 patients with primary insomnia and 54 age‐ and gender‐matched normal controls. The subjects who belonged to the Chinese population were subjected to polysomnography for three consecutive nights. Their sleep quality was assessed, and the genotypes of the 5‐hydroxytryptamine (5‐HT) gene polymorphism rs140700 were determined by the flight mass spectrometry.ResultsThe genotype distributions of the 5‐HT gene polymorphism rs140700 were in Hardy–Weinberg equilibrium in both patients and controls (P > 0.05). The allele and genotype distributions of this variant were comparable between the patients and controls in all subjects and between genders (all P > 0.05). The influence of rs140700 on percentage of stage 1 (P = 0.015) change and arousal index (P = 0.028) of primary insomnia was statistically significant. The logistic multi‐factor regression analysis results revealed that 5‐HT gene polymorphism rs140700 was not a risk factor for primary insomnia in the Chinese population (P = 0.589).ConclusionsThe 5‐HT gene polymorphism rs140700 may not be a susceptibility locus for primary insomnia in the Chinese population.
Pro‐environmental health behaviour and educational needs among pregnant women: A cross‐sectional survey
Authors: Jeong Geum Hee , Kim Hyun Kyoung 
Published: March 2020

AbstractAimTo investigate pro‐environmental health perceptions, behaviour and educational needs among pregnant women in Korea.BackgroundBased on concerns about the effects of environmental hazards and pollution on their babies and themselves, pregnant women behave in ways that protect their health. The framework of this study was based on Rogers' protection motivation theory.DesignCross‐sectional survey using questionnaires.MethodThe sample comprised 358 pregnant women recruited from July – August 2018 in South Korea. Participants were recruited from prenatal classes at two healthcare centres and patients receiving prenatal check‐ups at two women's hospitals.ResultsPerceived educational needs were ranked in the following order: particulate matter (23.7%), electromagnetic waves (11.7%), instant food (food additives) (9.0%) and environmental hormones (8.3%). Age (β = 0.17, p = .001), perceived severity (β = 0.19, p = .001) and response efficacy (β = 0.28, p < .001) affected pro‐environmental behaviour, which explained 23.0% of the variance.ConclusionEducational programs are necessary for mitigating environmental risks during pregnancy. Pregnant women engage in pro‐environmental behaviour influenced by pro‐environmental perceptions, especially perceptions of severity regarding environmental diseases and the efficacy of health behavioural responses. Nursing professionals can use these results to promote pro‐environmental health in pregnant women.ImpactThis study demonstrated that pregnant women need environmental health programs in prenatal education. Pro‐environmental perceptions regarding the severity of the environmental diseases and behavioural efficacy preceded the formation of environmental health behaviours. Nurses should support pregnant women's environmental health through educational interventions including air, electromagnetic, food pollution and endocrine disruptors.
Down‐regulated lncRNA AGAP2‐AS1 contributes to pre‐eclampsia as a competing endogenous RNA for JDP2 by impairing trophoblastic phenotype
Authors: Xu Yetao , Xia Xi , Jiang Ying , Wu Dan , Wang Sailan , Fu Shilong , Yang Nana , Zhang Yuanyuan , Sun Lizhou 
Published: March 2020

AbstractRecently, growing evidence has shown that aberrant long non‐coding RNA (lncRNA) expression in conjunction with an impaired trophoblastic phenotype could implicate the pathological process of pre‐eclampsia (PE). However, only a small portion of lncRNAs has been characterized with regard to the function and molecular mechanisms involved in PE. There are still gaps in the available knowledge; as a result, there are currently only a few applicable treatments for PE in the context of lncRNA. Here, we found that lncRNA AGAP2‐AS1 is abnormally down‐regulated in severe PE placenta tissues. Using human trophoblasts, we established that AGAP2‐AS1 knockdown could inhibit trophoblasts proliferation and invasion and promote cell apoptosis. Further, we showed that overexpression of AGAP2‐AS1 substantially stimulated the development of the trophoblastic phenotype. Through high‐throughput sequencing analysis, we demonstrated that silencing of AGAP2‐AS1 favourably regulated various genes which are relevant to trophoblastic growth and invasion. Mechanistically, AGAP2‐AS1 promoted the suppressor protein, Jun dimerization protein 2 (JDP2), by sponging miR‐574‐5p. Resultantly, further impairment of the trophoblastic phenotype was achieved by way of inhibiting cell growth, apoptosis and invasion. We also determined that the expression of AGAP2‐AS1 could be mediated by FOXP1. Our results showed that the down‐regulated expression of lncRNA AGAP2‐AS1 might serve as a key suppressor in PE via inhibition of JDP2 at the post‐transcriptional level by competing for miR‐574; thus, this presents a novel therapeutic strategy for PE.