bluuebunny/crossref_metadata_2025_split huggingface dataset:
H2A.Z is dispensable for both basal and activated transcription in post-mitotic mouse muscles
Authors: Belotti Edwige , Lacoste Nicolas , Simonet Thomas , Papin Christophe , Padmanabhan Kiran , Scionti Isabella , Gangloff Yann-Gaël , Ramos Lorrie , Dalkara Defne , Hamiche Ali , Dimitrov Stefan , Schaeffer Laurent 
Published: February 2020

Abstract
While the histone variant H2A.Z is known to be required for mitosis, it is also enriched in nucleosomes surrounding the transcription start site of active promoters, implicating H2A.Z in transcription. However, evidence obtained so far mainly rely on correlational data generated in actively dividing cells. We have exploited a paradigm in which transcription is uncoupled from the cell cycle by developing an in vivo system to inactivate H2A.Z in terminally differentiated post-mitotic muscle cells. ChIP-seq, RNA-seq and ATAC-seq experiments performed on H2A.Z KO post-mitotic muscle cells show that this histone variant is neither required to maintain nor to activate transcription. Altogether, this study provides in vivo evidence that in the absence of mitosis H2A.Z is dispensable for transcription and that the enrichment of H2A.Z on active promoters is a marker but not an active driver of transcription.
A novel transcriptional cascade is involved in Fzr-mediated endoreplication
Authors: Qian Wenliang , Li Zheng , Song Wei , Zhao Tujing , Wang Weina , Peng Jian , Wei Ling , Xia Qingyou , Cheng Daojun 
Published: February 2020

AbstractEndoreplication, known as endocycle, is a variant of the cell cycle that differs from mitosis and occurs in specific tissues of different organisms. Endoreplicating cells generally undergo multiple rounds of genome replication without chromosome segregation. Previous studies demonstrated that Drosophila fizzy-related protein (Fzr) and its mammalian homolog Cdh1 function as key regulators of endoreplication entrance by activating the anaphase-promoting complex/cyclosome to initiate the ubiquitination and subsequent degradation of cell cycle factors such as Cyclin B (CycB). However, the molecular mechanism underlying Fzr-mediated endoreplication is not completely understood. In this study, we demonstrated that the transcription factor Myc acts downstream of Fzr during endoreplication in Drosophila salivary gland. Mechanistically, Fzr interacts with chromatin-associated histone H2B to enhance H2B ubiquitination in the Myc promoter and promotes Myc transcription. In addition to negatively regulating CycB transcription, the Fzr-ubiquitinated H2B (H2Bub)-Myc signaling cascade also positively regulates the transcription of the MCM6 gene that is involved in DNA replication by directly binding to specific motifs within their promoters. We further found that the Fzr-H2Bub-Myc signaling cascade regulating endoreplication progression is conserved between insects and mammalian cells. Altogether, our work uncovers a novel transcriptional cascade that is involved in Fzr-mediated endoreplication.
A posteriori dietary patterns and their association with systemic low-grade inflammation in adults: a systematic review and meta-analysis
Authors: Norde Marina M , Collese Tatiana S , Giovannucci Edward , Rogero Marcelo M 
Published: February 2020

Abstract

Context
A posteriori dietary patterns are promising ways of uncovering potential public health strategies for the prevention of systemic, low-grade, inflammation-related, chronic noncommunicable diseases.

Objective
To investigate and summarize the current evidence on the association between a posteriori dietary patterns and systemic, low-grade inflammation in adults.

Data sources
MEDLINE, EMBASE, Web of Science, and LILACS were searched.

Data extraction
Data screening, extraction, and quality assessment were performed independently by 2 investigators. Meta-analysis with random effects was conducted. Differences and similarities between reduced rank regression–derived dietary patterns were assessed.

Results
Healthy dietary patterns are inversely and the Western dietary pattern is positively associated with inflammation (r = −0.13, 95% confidence interval −0.20 to −0.06; and r = 0.11, 95% confidence interval, 0.09–0.12, respectively). Reduced rank regression–derived anti-inflammatory dietary patterns are consistently characterized by high intake of fresh fruits and inflammatory dietary patterns are consistently characterized by high intake of red and processed meat and low intake of vegetables.

Conclusion
Favoring the substitution of a Westernized diet for a healthy diet may lower inflammation, which might improve the prevention of some chronic noncommunicable diseases.
The Politics of Musical Standardization in Nineteenth-Century France and Britain
Authors: Gillin Edward , Gribenski Fanny 
Published: February 2020

Abstract
This article examines mid-nineteenth-century Anglo-French relations through the prism of musical standardization. Bringing together perspectives from musicology, history of science, and political history, it demonstrates the holistic value of musical practices for the study of processes of political integration. In 1859, Napoléon III's government determined a national pitch to which musicians should tune their instruments. The following year, Britain's Society of Arts attempted to emulate this standard. Amid tense Anglo-French relations, British audiences interpreted the French pitch as a measure of the country's autocracy, and these political anxieties materialized through a redefinition of the standard. The challenges of introducing a musical pitch within a liberal political framework encountered in 1859 were subsequently echoed in debates over the reform of weights and measures following the 1860 free trade treaty between Britain and France. Both the economic and artistic integration of these countries involved the problem of how to regulate society within a laissez-faire state. Musical standardization has received little historiographical attention, but the regulation of this art offers insights into mid-nineteenth-century Anglo-French political culture. Entangled within complex network of industrial, institutional, and social structures, musical pitch demonstrates how problems of economic and social integration were inseparable from international and socio-political contexts.
Exploring Acceptance and Commitment Therapy for parents of preterm infants
Authors: Esser Kayla , Barreira Lesley , Miller Doug , Church Paige , Major Nathalie , Cohen Eyal , Orkin Julia 
Published: February 2020

Abstract
The start of a parenting journey in the neonatal intensive care unit (NICU) presents many stressors to parents. Previous research has shown parents of infants admitted to the NICU experience heightened stress, anxiety, and depression. Mental health support varies across Canadian NICUs with mixed results. One promising intervention that has not been explored in the NICU is Acceptance and Commitment Therapy (ACT), a behavioural therapy that has had positive mental health-related outcomes in similar parental populations. ACT differs from previous mental health interventions such as traditional Cognitive Behavioural Therapy (CBT) as it involves mindfulness and acceptance to increase psychological flexibility. Increased psychological flexibility is linked to greater emotional well-being, a higher quality of life, and decreased stress, anxiety, and depression. There is a need for research investigating the utility of ACT in improving mental health outcomes for parents of preterm infants.
Out-of-Sample Performance of Mutual Fund Predictors
Authors: Jones Christopher S , Mo Haitao 
Published: February 2020

Abstract
We analyze the out-of-sample performance of variables shown to forecast future mutual fund alphas. The degree of predictability, as measured by alpha spreads from quintile sorts or cross-sectional regression slopes, falls by at least half post-sample. These declines appear to be primarily the result of changes in the level of arbitrage activity in the market, with mutual fund competition appearing to play a secondary role. We find no evidence that the declines are the result of data snooping or learning by investors or fund managers. Finally, we show that corporate bond fund performance exhibits similar dependence on measures of bond market arbitrage activity.
Lack of Multidrug Resistance-associated Protein 4 Prolongs Partial Hepatectomy-induced Hepatic Steatosis
Authors: Donepudi Ajay C , Smith Gregory J , Aladelokun Oladimeji , Lee Yoojin , Toro Steven J , Pfohl Marisa , Slitt Angela L , Wang Li , Lee Ji-Young , Schuetz John D , Manautou José E 
Published: February 2020

Abstract
Multidrug resistance-associated protein 4 (Mrp4) is an efflux transporter involved in the active transport of several endogenous and exogenous chemicals. Previously, we have shown that hepatic Mrp4 expression increases following acetaminophen overdose. In mice, these increases in Mrp4 expression are observed specifically in hepatocytes undergoing active proliferation. From this, we hypothesized that Mrp4 plays a key role in hepatocyte proliferation and that lack of Mrp4 impedes liver regeneration following liver injury and/or tissue loss. To evaluate the role of Mrp4 in these processes, we employed two-third partial hepatectomy (PH) as an experimental liver regeneration model. In this study, we performed PH-surgery on male wildtype (C57BL/6J) and Mrp4 knockout mice. Plasma and liver tissues were collected at 24, 48, and 72 h postsurgery and evaluated for liver injury and liver regeneration endpoints, and for PH-induced hepatic lipid accumulation. Our results show that lack of Mrp4 did not alter hepatocyte proliferation and liver injury following PH as evaluated by Ki-67 antigen staining and plasma alanine aminotransferase levels. To our surprise, Mrp4 knockout mice exhibited increased hepatic lipid content, in particular, di- and triglyceride levels. Gene expression analysis showed that lack of Mrp4 upregulated hepatic lipin1 and diacylglycerol O-acyltransferase 1 and 2 gene expression, which are involved in the synthesis of di- and triglycerides. Our observations indicate that lack of Mrp4 prolonged PH-induced hepatic steatosis in mice and suggest that Mrp4 may be a novel genetic factor in the development of hepatic steatosis.
Stochastic discrete epidemic modeling of COVID-19 transmission in the Province of Shaanxi incorporating public health intervention and case importation
Authors: Tang Sanyi , Tang Biao , Bragazzi Nicola Luigi , Xia Fan , Li Tangjuan , He Sha , Ren Pengyu , Wang Xia , Peng Zhihang , Xiao Yanni , Wu Jianhong 
Published: February 2020

AbstractBefore the lock-down of Wuhan/Hubei/China, on January 23rd 2020, a large number of individuals infected by COVID-19 moved from the epicenter Wuhan and the Hubei province due to the Spring Festival, resulting in an epidemic in the other provinces including the Shaanxi province. The epidemic scale in Shaanxi was comparatively small and with half of cases being imported from the epicenter. Based on the complete epidemic data including the symptom onset time and transmission chains, we calculate the control reproduction number (1.48-1.69) in Xi’an. We could also compute the time transition, for each imported or local case, from the latent, to infected, to hospitalized compartment, as well as the effective reproduction number. This calculation enables us to revise our early deterministic transmission model to a stochastic discrete epidemic model with case importation and parameterize it. Our model-based analyses reveal that the newly generated infections decay to zero quickly; the cumulative number of case-driven quarantined individuals via contact tracing stabilize at a manageable level, indicating that the intervention strategies implemented in the Shaanxi province have been effective. Risk analyses, important for the consideration of “resumption of work”, show that a large second outbreak is expected if the level of case importation remains at the same level as between January 10th and February 4th 2020. However, if the case importation decreases by 30%, 60% and 90%, the second outbreak if happening will be of small-scale assuming contact tracing and quarantine/isolation remain as effective as before. Finally, we consider the effects of intermittent inflow with a Poisson distribution on the likelihood of multiple outbreaks. We believe the developed methodology and stochastic model provide an important model framework for the evaluation of revising travel restriction rules in the consideration of resuming social-economic activities while managing the disease control with potential case importation.
Genomic Sequencing for Newborn Screening: Results of the NC NEXUS Project
Authors: Roman Tamara S. , Crowley Stephanie B. , Roche Myra I. , Foreman Ann Katherine M. , O’Daniel Julianne M. , Seifert Bryce A. , Lee Kristy , Brandt Alicia , Gustafson Chelsea , DeCristo Daniela M. , Strande Natasha T. , Ramkissoon Lori , Milko Laura V. , Owen Phillips , Roy Sayanty , Xiong Mai , Paquin Ryan S. , Butterfield Rita M. , Lewis Megan A. , Souris Katherine J. , Bailey Donald B. , Rini Christine , Booker Jessica K. , Powell Bradford C. , Weck Karen E. , Powell Cynthia M. , Berg Jonathan S. 
Published: February 2020

AbstractNewborn screening (NBS) was established as a public health program in the 1960’s and is crucial for facilitating detection of certain medical conditions in which early intervention can prevent serious, life-threatening health problems. Genomic sequencing can potentially expand the screening for rare hereditary disorders, but many questions surround its possible use for this purpose. We examined the use of exome sequencing (ES) for NBS in theNorthCarolinaNewbornExome Sequencing forUniversalScreening (NC NEXUS) project, comparing the yield from ES used in a screening versus a diagnostic context. We enrolled healthy newborns and children with metabolic diseases or hearing loss (106 participants total). ES confirmed the participant’s underlying diagnosis in 15 out of 17 (88%) children with metabolic disorders, and in 5 out of 28 (∼18%) children with hearing loss.We discovered actionable findings in 4 participants that would not have been detected by standard NBS. A subset of parents was eligible to receive additional information for their child about childhood-onset conditions with low or no clinical actionability, clinically actionable adult-onset conditions, and carrier status for autosomal recessive conditions. We found pathogenic variants associated with hereditary breast and/or ovarian cancer in 2 children, a likely pathogenic variant in the gene associated with Lowe syndrome in one child, and an average of 1.8 reportable variants per child for carrier results. These results highlight the benefits and limitations of using genomic sequencing for NBS and the challenges of using such technology in future precision medicine approaches.
Evaluation of the clinical characteristics of suspected or confirmed cases of COVID-19 during home care with isolation: A new retrospective analysis based on O2O
Authors: Xu Hui , Huang Sufang , Liu Shangkun , Deng Juan , Jiao Bo , Ai Ling , Xiao Yaru , Yan Li , Li Shusheng 
Published: February 2020

SummaryBackgroundThe recent outbreak of the novel coronavirus in December 2019 (COVID-19) has activated top-level response nationwide. We developed a new treatment model based on the online-to-offline (O2O) model for the home isolated patients, because in the early stages the medical staff were insufficient to cope with so many patients.MethodsIn this single-centered, retrospective study, we enrolled 48 confirmed/suspected COVID-19 patients who underwent home isolation in Wuhan between January 6 and January 31, 2020. By WeChat and online document editing all patients were treated with medical observation scale. The clinical indications such as Fever, Muscle soreness, Dyspnea and Lack of strength were collected with this system led by medical staff in management, medicine, nursing, rehabilitation and psychology.FindingsThe mean age of 48 patients was 39·08±13·88 years, 35(72·9%) were women. Compared with non-hospitalized patients, inpatients were older(≥70years, 2·4% vs 33·3%, P<0·04). All inpatients had fever, 50% inpatients had coughs and showed infiltration in both lungs at the time of diagnosis. 33·3% inpatients exhibited negative changes in their CT results at initial diagnosis. The body temperature of non-hospitalized patients with mild symptoms returned to normal by day 4-5. While dyspnea peaked on day 6 for non-hospitalized patients with mild symptoms, it persisted in hospitalized patients and exacerbated over time. The lack of strength and muscle soreness were both back to normal by day 4 for non-hospitalized patients.InterpretationMonitoring the trends of symptoms is more important for identifying severe cases. Excessive laboratory data and physical examination are not necessary for the evaluation of patients with mild symptoms. The system we developed is the first to convert the subjective symptoms of patients into objective scores. This type of O2O, subjective-to-objective strategy may be used in regions with similar highly infectious diseases to minimize the possibility of infection among medical staff.
Rapid Molecular Detection of SARS-CoV-2 (COVID-19) Virus RNA Using Colorimetric LAMP
Authors: Zhang Yinhua , Odiwuor Nelson , Xiong Jin , Sun Luo , Nyaruaba Raphael Ohuru , Wei Hongping , Tanner Nathan A. 
Published: February 2020

ABSTRACTThe ability to detect an infectious agent in a widespread epidemic is crucial to the success of quarantine efforts in addition to sensitive and accurate screening of potential cases of infection from patients in a clinical setting. Enabling testing outside of sophisticated laboratories broadens the scope of control and surveillance efforts, but also requires robust and simple methods that can be used without expensive instrumentation. Here we report a method to identify SARS-CoV-2 (COVID-19) virus RNA from purified RNA or cell lysis using loop-mediated isothermal amplification (LAMP) using a visual, colorimetric detection. This test was additionally verified using RNA samples purified from respiratory swabs collected from COVID-19 patients in Wuhan, China with equivalent performance to a commercial RT-qPCR test while requiring only heating and visual inspection. This simple and sensitive method provides an opportunity to facilitate virus detection in the field without a requirement for complex diagnostic infrastructure.
Modelling the coronavirus disease (COVID-19) outbreak on the Diamond Princess ship using the public surveillance data from January 20 to February 20, 2020
Authors: Zhao Shi , Cao Peihua , Gao Daozhou , Zhuang Zian , Chong Marc KC , Cai Yongli , Ran Jinjun , Wang Kai , Lou Yijun , Wang Weiming , Yang Lin , He Daihai , Wang Maggie H 
Published: February 2020

AbstractThe novel coronavirus disease 2019 (COVID-19) outbreak on the Diamond Princess ship has caused over 634 cases as of February 20, 2020. We model the transmission process on the ship with a stochastic model and estimate the basic reproduction number at 2.2 (95%CI: 2.1-2.4). We estimate a large dispersion parameter than other coronaviruses, which implies that the virus is difficult to go extinction. The epidemic doubling time is at 4.6 days (95%CI: 3.0-9.3), and thus timely actions were crucial. The lesson learnt on the ship is generally applicable in other settings.
The Effects of “Fangcang, Huoshenshan, and Leishenshan” Hospitals and Temperature on the Mortality of COVID-19
Authors: Cai Yuwen , Huang Tianlun , Liu Xin , Xu Gaosi 
Published: February 2020

AbstractBackgroundIn December 2019, a novel coronavirus disease (COVID-19) broke out in Wuhan, China, however, the factors affecting the mortality remain unclear.MethodsThirty-two days of data that were shared by China National Health Commission and China Weather Net were collected using standard forms. The difference in the mortality of confirmed and severe cases before and after the use of “Fangcang, Huoshenshan, and Leishenshan” makeshift hospitals (MSHs) was tested using Mann-Whitney U test. We also studied whether air temperature (AT) could affect the above outcomes of COVID-19 cases by performing Spearman’s analysis.ResultsEight days after the use of MSHs, the mortality of confirmed cases was significantly decreased both in Wuhan (U = 1, P < 0.001) and Hubei (U = 0, P < 0.001), while in non-Hubei regions, as a contrast, the mortality of confirmed cases remained unchanged (U = 40, P = 0.139). However, another eight days later, changes in the mortality in non-Hubei regions also became significant (U = 73, P = 0.039). Mortality of confirmed cases was found to be significantly correlated with AT both in Wuhan (r = −0.441, P = 0.012) and Hubei (r = −0.440, P = 0.012).ConclusionsOur findings indicated that both the use of MSHs and the rise of AT were beneficial to the survival of COVID-19 cases.
Coordinated prefrontal state transition leads extinction of reward-seeking behaviors
Authors: Russo Eleonora , Ma Tianyang , Spanagel Rainer , Durstewitz Daniel , Toutounji Hazem , Köhr Georg 
Published: February 2020

ABSTRACTExtinction learning suppresses conditioned reward responses and is thus fundamental to adapt to changing environmental demands and to control excessive reward seeking. The medial prefrontal cortex (mPFC) monitors and controls conditioned reward responses. Usingin vivomultiple single-unit recordings of mPFC we studied the relationship between single-unit and population dynamics during different phases of an operant conditioning task. To examine the fine temporal relation between neural activity and behavior, we developed a model-based statistical analysis that captured behavioral idiosyncrasies. We found that single-unit responses to conditioned stimuli changed throughout the course of a session even under stable experimental conditions and consistent behavior. However, when behavioral responses to task contingencies had to be updated during the extinction phase, unit-specific modulations became coordinated across the whole population, pushing the network into a new stable attractor state. These results show that extinction learning is not associated with suppressed mPFC responses to conditioned stimuli, but is driven by single-unit coordination into population-wide transitions of the animal’s internal state.
Sex differences in clinical findings among patients with coronavirus disease 2019 (COVID-19) and severe condition
Authors: Li Jing , Zhang Yinghua , Wang Fang , Liu Bing , Li Hui , Tang Guodong , Chang Zhigang , Liu Aihua , Fu Chunyi , Gao Jing , Li Jing 
Published: February 2020

AbstractObjectiveTo compare the sex differences in the clinical findings among patients with severe coronavirus disease 2019 (COVID-19).MethodsWe retrospectively collected data of 47 patients diagnosed as severe type of COVID-19 from February 8 to 22, 2020, including demographics, illness history, physical examination, laboratory test, management, and compared differences between men and women.ResultsOf the 47 patients, 28 (59.6%) were men. The median age was 62 years, and 30 (63.8%) had comorbidities. The initial symptoms were mainly fever (34 [72.3%]), cough (36 [76.6%]), myalgia (5 [10.6%]) and fatigue (7 [14.9%]). Procalcitonin level was higher in men than in women (0.08 vs. 0.04ng/ml, p=0.002). N-terminal-pro brain natriuretic peptide increased in 16 (57.1%) men and 5 (26.3%) women (p=0.037). Five men (17.9%) had detected positive influenza A antibody, but no women. During 2-week admission, 5 (17.9%) men and 1 (5.3%) woman were reclassified into the critical type due to deterioration. Mortality was 3.6% in men and 0 in women respectively. Four (21.1%) women and one man (3.6%) recovered and discharged from hospital.ConclusionSex differences may exist in COVID-19 patients of severe type. Men are likely to have more complicated clinical condition and worse in-hospital outcomes as compared to women.