bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fundus Photography, Fundus Fluorescein Angiography, and Optical Coherence Tomography of Healthy Cynomolgus Monkey, New Zealand Rabbit, Sprague Dawley Rat, and BALB/c Mouse Retina
Authors: Xian Bikun , Zhao Minglei , Peng Yuting , Wang Wencong , Li Zhiquan , Zhang Henig , Li Weihua , Huang Bing 
Published: May 2020

Abstract
Background

A variety of experimental animal models are used in basic ophthalmological research to elucidate physiological mechanisms of vision and disease pathogenesis. The choice of animal model is based on the measurability of specific parameters or structures, the applicability of clinical measurement technologies, and the similarity to human eye function. Studies of eye pathology usually compare optical parameters between a healthy and altered state, so accurate baseline assessments are critical, but few reports have comprehensively examined the normal anatomical structures and physiological functions in these models.
Methods

Three cynomolgus monkeys, six New Zealand rabbits, ten Sprague Dawley (SD) rats, and BALB/c mice were examined by fundus photography (FP), fundus fluorescein angiography (FFA), and optical coherence tomography (OCT).
Results

Most retinal structures of cynomolgus monkey were anatomically similar to the corresponding human structures as revealed by FP, FFA, and OCT. New Zealand rabbits have large eyeballs, but they have large optic disc and myelinated retinal nerve fibers in their retina, and the growth pattern of retinal vessels were also different to the human retina. Unlike monkeys and rabbits, the retinal vessels of SD rats and BLAB/c mice were widely distributed and clear. And the OCT preformance of them were similar with human beings.
Conclusion

Monkey is a good model to study changes in retinal structure associated with fundus disease, rabbits are not suitable for studies on retinal vessel diseases and optic nerve diseases, and rats and mice are good models for retinal vascular diseases. These measures will help guide the choice of model and measurement technology and reduce the number of experimental animals required.
Diet Quality and Mental Health Amongst Acute Inpatient Psychiatric Patients
Authors: Gill Raveena , Tyndall Sean F , Vora Darshini , Hassan Rashedul , Megna James , Leontieva Luba 
Published: May 2020

Abstract
BackgroundPoor diet quality has been found to be prevalent in people with mental illness [71] and even contribute to mental illness [71]. The purpose of this exploratory study is to examine the relationship between diet quality, mental health problems, socioeconomic variables, physical activity level, and body mass index amongst acute psychiatric inpatients.MethodThe study sample were 100 adult inpatients. Tools administered were Eating Habit Questionnaires (Dana Farber Institute) and the KIDMed index to assess food compliance in accordance with a Mediterranean diet. The Index marks all individuals as either having an “unhealthy score” or “healthy score”. The study utilized a descriptive correlation research design.ResultsThe results showed that 75% of the patients displayed an unhealthy score as per the KIDMed index. The findings displayed an inverse relationship of BMI and the KIDMed score. There was a significant positive correlation between the level of education and the KIDMed score. There is a positive correlation towards increased consumption of sugar in schizophrenic patients. There was no statistical significance between age, gender, income and physical activity with regards to the KIDMed score.ConclusionPsychiatric inpatients display unhealthy eating habits which is likely both an effect of and a contributing factor to the various mental illnesses. Prospective interventional randomized controlled studies are necessary for clarification of cause-effect relationships.
Cross-Calibration of Bone Mineral Densities and Body Composition Between GE-Lunar Prodigy and Osteosys Primus
Authors: Ha Yong-Chan , YOO JUN-IL 
Published: May 2020

Abstract
Background:The aim of this study was to investigate the correlation between bone mineral density (BMD) and body composition measured by the Osteosys Primus® and the GE Lunar Prodigy® and to calculate the conversion formula between the two devices.Methods:The forty subjects were men and women in aged 20-29 years old. Study subjects with a body mass index (BMI) greater than or equal to 17 kg / m2 and less than or equal to 35 kg / m2with a negative pregnancy test at the time of screening, and participants who voluntarily participated and agreed to provide written consent were included in the study. The study exclusion criteria were patients with scoliosis, osteoarthritis, rigid vertebral osteomalacia, or other clinical vertebral deformities, adverse events after previous DXA or radiography, pregnant or lactating women, those with artificial pacemakers, or implanted cardiac pacemakers, and those with mental illnesses, such as severe depression. All participants were scanned twice on both the Osteosys Primus (OsteoSys, Seoul, Korea) and the GE-Lunar Prodigy (Madison, WI, USA) DXA systems using the manufacturers’ standard scanning and positioning protocols. Results:Compared to the GE Lunar device, the mean Osteosys fat mass was overestimated to be 12.1 % (1776.9 g) in the whole body, 5.1 % (163.9 g) in gynoid, and 6.7 % (87.2 g) in android. Compared with the GE Lunar device, the mean BMDs of the Osteosys Primus were underestimated to be 2.3 % (0.023 g/cm2) in the whole body and 3.1 % (0.035 g/cm2) in L1-4. Compared with the GE Lunar device, the mean lean mass derived by the Osteosys Primus were underestimated to 2.3 % (1045.3 g) in the total body, 3.8 % (179.4 g) in arms, and 7.7 % (1104.8 g) in legs, respectively. There were a strong correlation of BMD and body composition between both groups.Conclusion:There was a very high correlation of BMD and muscle mass between theOsteosys Primus and the GE Lunar Prodigy.
Giving women WOICE postpartum: Prevalence of maternal morbidity using the WHO-WOICE instrument
Authors: Lamus Martha Narvaez , Lozano Stephanie , MPoca Charles , Guida Jose Paulo , Parpinelli Mary Angela , Cecatti Jose Guilherme , Vidarte Maria Escobar , Say Lale , Chou Doris , Costa Maria Laura 
Published: May 2020

Abstract
BackgroundThere are no accurate estimates of the prevalence of non-severe maternal morbidities. Given the lack of instruments to fully assess these morbidities, the World Health Organization (WHO) developed an instrument called WOICE.ObjectiveTo evaluate the prevalence of non-severe maternal morbidities in puerperal women and analyses factors associated to impaired clinical, social and mental health conditions.Method﻿A cross-sectional study with 519 postpartum women in a single encounter 6 to 12 weeks postpartum. The WOICE questionnaire included three sections: the first with maternal and obstetric history, sociodemographic data, risk and environment factors, violence and sexual health; The second considers functionality and disability, general symptoms and mental health; and the third includes data on physical and laboratory tests. Data collection was supported by Tablets with REDCAP software. Initially, a descriptive analysis was performed, with general prevalence of all variables contained in the WOICE, including scales on anxiety and depression (GAD-7 and PHQ-9- altered if ≥ 10), functionality (WHODAS- altered when ≥ 37.4) and data on violence and substance use. Subsequently, an evaluation of cases with alterations was performed, with a logistic regression to investigate factors associated to impaired non-clinical and clinical conditions.Results517 women were included, majority (54.3%) multiparous, ages between 20 and 34 years (65.4%) and with partner (75,6%). Over a quarter had (26.2%) preterm birth, however good perinatal outcomes. Around a third (30.2%) reported health problems informed by the physician, although more than 80% considered having good or very good health. About 10% reported any substance and 5.9% suffered violence. Anxiety was identified in 19.8% of cases, depression in 36.9% and altered functioning in 4.4% of women. Logistic regression identified that poor overall health rating was associated to increased anxiety/depression and impaired functioning. Having a partner reduces the perception of women on the presence of clinical morbidities.ConclusionDuring postpartum care, women presented high frequency of anxiety and depression and relevant frequency of substance use and violence. These aspects of women´s health need further evaluation and specific interventions to improve quality of care.
Absence of False Acetabulum is a Potent Indicator for Predicting the Use of Subtrochanteric Osteotomy in Total Hip Arthroplasty for High Dislocated Hips
Authors: Sun Jing-yang , Zhang Guo-qiang , Du Yin-qiao , Shen Jun-min , Zhou Yong gang , Wang Yan 
Published: May 2020

Abstract
Introduction: This study aimed to evaluate the predictive values of false acetabulum for assessment of whether to use subtrochanteric osteotomy.Materials and Methods: We retrospectively included a total of 182 patients (232 hips) affected by Crowe type IV developmental dysplasia who underwent primary THA with modular cementless stem from April 2008 to May 2019 in our institution. Based on radiographs and operative notes, we found 175 hips were performed with subtrochanteric osteotomy and 57 without subtrochanteric osteotomy, which was named (subtrochanteric osteotomy) STO group and non-STO group, respectively. The predictive values of absence of false acetabulum and distalization of greater trochanter were analyzed using receiver operating characteristic (ROC) curves.Results: ROC curves showed that absence of false acetabulum and distalization of greater trochanter had the AUCs of 0.957 and 0.987, respectively. And there was no statistical significance in the difference of these two AUCs (P=0.392). The optimal threshold for the distalization of greater trochanter was 4.83 cm, which resulted in a sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) of 92.6%, 100%, 100%, and 81.4%, respectively. For the absence of false acetabulum, the sensitivity, specificity, PPV, and NPV were 94.9%, 96.5%, 98.8%, and 85.9%, respectively.Conclusions: The absence of false acetabulum may be a potent indicator in predicting the use of subtrochanteric osteotomy in high dislocated hips.
Comparison of Sagittal Spinal Alignment on Standing Plain X-Rays and Supine MRI in Degenerative Lumbar Disease
Authors: Wang Yulu , Bai Jinwu , Pan Zhijun , Xue Deting 
Published: May 2020

Abstract
Background:The purpose of present study is to examine the possible correlation between standing plain X-rays and supine MRI for evaluating spinal sagittal alignment in degenerative lumbar disease.Methods: The characteristics and images of 64 patients with degenerative lumbar diseases were reviewed retrospectively. The thoracolumbar junction kyphosis (TJK), lumbar lordosis (LL), and sacral slope (SS) were measured on lateral plain X-rays and by magnetic resonance imaging (MRI). Inter- and intra-observer reliability were tested using intra-class correlation coefficients. Results: The results suggested that TJK measurements obtained from MRI tended to underestimate the radiographic measures by 2°, whereas SS measurements obtained from MRI tended to overestimate the radiographic measures by 2°. The LL measurements obtained from MRI were approximately equal to the radiographic measures. The X-ray and MRI measurements were linearly related. Conclusions:In conclusion, supine MRI can be directly translated into sagittal alignment angle measurements obtained from standing X-rays with an acceptable degree of accuracy. This can avoid the impaired view caused by the overlapping ilium, while reducing the patient’s exposure to radiation.
Evaluating drug prescription patterns in children according to World Health Organisation's indicators, Kigali, Rwanda: A cross-sectional study
Authors: HABIMANA Gaspard , Nyandwi Theogene , Kabayiza Jean Claude , Kayumba Pierre Claver 
Published: May 2020

Abstract
Background Irrational drug use is a worldwide problem at all levels of health care, especially in hospitals. Prescribers in community tend to omit hospital prescribing practices, thus this problem is present at all levels of health care. Use of medicines can be greatly improved and wastage reduced if simple principles of drug management are followed. To help in solving this problem, World Health Organization (WHO) has designed prescribing core indicators that were used to evaluate drug prescription patterns in Rwanda Social Security Board (RSSB) affiliated patients.Material and Methods A retrospective cross-sectional study with quantitative analysis was carried out for a period of six months. Among the 1000 prescriptions collected, 18 of them were rejected for not fulfilling the inclusion criteria, the remaining 982 prescriptions were almost distributed equally from either private and public hospital or clinics. The study analyzed prescriptions from health facilities located in Kigali City, Rwanda. The results were presented in table with comparisons with the WHO prescription indicators.Results The average number of drugs per prescription is 2.4 which is higher than 1.8 recommended by WHO. The percentages of drugs prescribed with a generic name and those from National Essential drug List are 28.5% and 61.1% respectively, which are very low compared to WHO target of 100% for both core prescribing indicators. The percentage of encounters with an antibiotic prescribed is 42.5% and the encounters who received an injections account 1.2%.Conclusion This study revealed that there is a big gap in implementing WHO core indicators of prescription and concerned stakeholders should take measures to address this issue.
Atorvastatin Promotes Bone Formation in Aged apoE–/– Mice through the Sirt1–Runx2 Axis
Authors: Hong Wei , Wei Zhanying , Qiu Zhaohui , Li Zheng , Fu Chensheng , Ye Zhibin , xu xiaoya 
Published: May 2020

Abstract
Background
Statins are the most widely used drugs in elderly patients, the most common clinical application of statins is in aged hyperlipemia patients. There are few studies on the effects and mechanisms of statins on bone in elderly mice with hyperlipemia. The study is to examine the effects of atorvastatin on bone phenotypes and metabolism in aged apolipoprotein E-deficient (apoE–/–) mice, and the possible mechanisms involved in these changes.
Methods
Twenty-four 60-week-old apoE–/– mice were randomly allocated to two groups. Twelve mice were orally gavaged with atorvastatin (10 mg/kg body weight/day) for 12 weeks; the others served as the control group. Bone mass and skeletal microarchitecture were determined using micro-CT. Bone metabolism was assessed by serum analyses, qRT-PCR, and Western blot. Bone marrow-derived mesenchymal stem cells (BMSCs) from apoE–/– mice were differentiated into osteoblasts and treated with atorvastatin and Sirt1 inhibitor EX-527.
Results
The results showed that long-term administration of atorvastatin increases bone mass and improves bone microarchitecture in trabecular bone but not in cortical bone. Furthermore, the serum bone formation marker osteocalcin (OCN) was ameliorated by atorvastatin, whereas the bone resorption marker tartrate-resistant acid phosphatase 5b (Trap5b) did not appear to be impacted by atorvastatin treatment. The mRNA expression of Sirt1, runt-related transcription factor 2 (Runx2), alkaline phosphatase (ALP) and OCN in bone tissue were increased after atorvastatin administration. Western blot showed same trend in Sirt1 and Runx2. The in vitro study showed that when BMSCs from apoE–/– mice were pretreated with EX527, the higher expression of Runx2, ALP and OCN activated by atorvastatin decreased significantly or showed no difference compared with the control. The protein expression of Runx2 showed same trend.
Conclusions
Accordingly, the current study validates the hypothesis that atorvastatin can increase bone mass and promote osteogenesis in aged apoE−/− mice by regulating the Sirt1–Runx2 axis.
Mirabilite with ice pack after Total Knee Arthroplasty: A Randomized Controlled Trial Study
Authors: Zhong Ying , Zheng Cheng , Du Wenxi , Zheng Jiahua , Xu Shanchun , Tong Peijian 
Published: May 2020

Abstract
Background
Total knee arthroplasty (TKA) is a well-established procedure for end-state arthritis of knee with complications such as swelling and pain. The aim of this study is to evaluate the effect of mirabilite with ice pack versus ice pack alone on relieving pain, reducing swelling, range of motion (ROM), and serum CRP level in patients after TKA.
Methods
Eighty patients undergoing primary unilateral TKA were randomly assigned to two groups (MIP group and WIP group). We used VAS to measure knee pain at 24 h, 48 h, and 72 h after the surgery respectively. Knee swelling degree was evaluated by measuring the girth of leg at the center of patella and 10 cm above and below it at the same frequency. The active ROM of the knee was measured by using a universal goniometer of plastic material at 72 h postoperatively. The serum CRP level was also measured at 72 h postoperatively.
Results
The MIP group showed statistically significant lower knee girth at 48 h (p༜0.05), 72 h (p < 0.05) postoperatively and VAS score at 72 h (P = 0.018) at postoperatively after TKA than WIP group. The ROM of MIP group were significantly wider than WIP group (P = 0.024). The CRP level (P = 0.036) and length of stay (LOS) (P = 0.037) of MIP group were significantly lower than WIP group.
Conclusions
Mirabilite with ice pack after TKA showed superiority in relieving pain, reducing swelling, and improving ROM than ice pack alone.
Trial registration:
Chinese Clinical Trial Registry (ChiCTR), ChiCTR-OCN-15006356. Registered 6 MAY 2015, http://www.chictr.org.cn/showproj.aspx?proj=10927
Application of magnesium sulphate as a " multimodal general anesthesia" protocol in patients undergoing hysteroscopy: a randomized controlled study
Authors: Gao Peng-fei , Lin Jing-yan , Wang Shun , Zhang Yun-feng , Wang Guo-qiang , Xu Qi , Guo Xiao 
Published: May 2020

Abstract
Background The purpose of the study was to applicate magnesium sulphate as a " multimodal general anesthesia" protocol to reduce perioperative opioids requirements in patients undergoing hysteroscopy. Methods 70 patients scheduled for hysteroscopy were randomly divided into 2 groups. Patients in the Group M received intravenous magnesium sulfate 50 mg/kg in 100 ml of isotonic saline over 15 min before propofol injection and then 15 mg/kg per hour by continuous intravenous infusion. Patients in the control group received an equal volume of normal saline as placebo. All patients were anesthetized under a BIS guided monitored anesthesia care with propofol and fentanyl. Intraoperative anesthetic agent requirements and hemodynamic variables were recorded. Postoperative pain score was assessed with verbal numerical rating scale (VNRS) 1 min, 15 min, 30 min, 1 hour and 4 hours after recovery of consciousness. Results Postoperative serum magnesium concentrations in Group C were significantly declined than preoperative levels (0.86 ± 0.06 to 0.80 ± 0.08 mmol/L, P=0.001) while there was no statistical change in Group M (0.86 ± 0.07 to 0.89 ± 0.07 mmol/L, P=0.129). The total dose of fentanyl given to patients in Group M was less than the one administered to Group C (100 (75-150) vs 145 (75-175) μg, median (range); P < 0.001). Fewer patients in Group M required additional analgesic postoperatively. In addition, patients receiving magnesium displayed less VNRS scores at 15 min, 30 min, 1 hour and 4 hours postoperatively. Conclusions Intravenous infusion of magnesium sulphate significantly reduces perioperative opioids requirements. Meanwhile, it was beneficial to reduce postoperative pain and maintain stable of serum magnesium concentration after the procedure.
Generation of Dyskeratosis Congenita-like Hematopoietic Stem Cells through the Stable Inhibition of DKC1
Authors: Carrascoso-Rubio Carlos , Zittersteijn Hidde A. , Pintado-Berninches Laura , Fernández-Varas Beatriz , Lozano M. Luz , Manguan-Garcia Cristina , Sastre Leandro , Bueren Juan A. , Perona Rosario , Guenechea Guillermo 
Published: May 2020

Abstract
Dyskeratosis congenita is a rare telomere biology disorder, which results in different clinical manifestations, including severe bone marrow failure. To date, the only curative treatment for bone marrow failure in dyskeratosis congenita patients is allogeneic hematopoietic stem cell transplantation. However due to the toxicity associated to allogeneic hematopoietic stem cell transplantation in dyskeratosis congenita, new non-toxic therapies are recommended to improve the life expectancy of these patients. Since bone marrow biopsies are not routinely performed during the follow-up of dyskeratosis congenita patients, the availability of dyskeratosis congenita hematopoietic stem cells constitutes a major limitation in the development of new hematopoietic therapies for dyskeratosis congenita. Here we aimed at generating dyskeratosis congenita-like human hematopoietic stem cells in which the efficacy of new therapies could be investigated. X-linked dyskeratosis congenita is one of the most frequent variants of dyskeratosis congenita and is associated with an impaired expression of the DKC1 gene. In this study we thus generated dyskeratosis congenita-like hematopoietic stem cells based on the stable knock-down of DKC1 in human CD34+ cells, using lentiviral vectors encoding for DKC1 short hairpin RNAs. At a molecular level, DKC1-interfered CD34+ cells showed a decreased expression of TERC, as well as a diminished telomerase activity and increased DNA damage. Moreover, DKC1-interfered human CD34+ cells showed defective clonogenic ability and were incapable of repopulating the hematopoiesis of immunodeficient NSG mice. The development of dyskeratosis congenita-like hematopoietic stem cells will facilitate the understanding of the molecular and cellular basis of the bone marrow failure characteristic of dyskeratosis congenita patients, and will serve as a platform for the development of new hematopoietic therapies for dyskeratosis congenita patients.
Identification of a 9-Gene Prognostic signature for breast cancer
Authors: Tian Zelin , Tang Jianing , Liao Xing , Yang Qian , Wu Yumin , Wu Gaosong 
Published: May 2020

Abstract
Background Breast cancer (BRCA) is the most common cancer among women worldwide and results in the second leading cause of woman cancer death.Methods This study sought to develop a prognostic gene signature to predict the prognosis of patients with BRCA. Studies were performed using the genome-wide data of BRCA patients from the Gene Expression Omnibus dataset (GSE20685, GSE42568, GSE20711, GSE88770). Univariate COX regression analysis was used to determine the association between gene expression levels and overall survival(OS) in each dataset. Taking P value < 0.05 as the inclusion criterion, the common genes in all datasets were selected as prognostic genes, and a 9-gene prognostic signature was developed.Results The Kaplan-Meier survival curve was constructed using log-rank test to assess survival differences. The overall survival of patients in the low-risk group was significantly higher than that in the high-risk group. ROC analysis showed that this 9-gene signature showed good diagnostic efficiency both in overall survival(OS) and disease free survival(DFS). The 9-gene signature was further validated using GSE16446 dataset. In addition, multiple Cox regression analysis showed that this 9-gene signature was an independent risk factor. Finally, we established a nomogram that integrates conventional clinicopathological features and 9-gene signature. The analysis of the calibration plots showed that the nomogram has good performance.Conclusions This study has developed a reliable 9-gene prognostic signature, which is of great value in predicting the prognosis of BRCA and will help to make personalized treatment decisions for patients at different risk score.