bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparison of Proteomic Assessment Methods in Multiple Cohort Studies
Authors: Raffield Laura M. , Dang Hong , Pratte Katherine A. , Jacobson Sean , Gillenwater Lucas A. , Ampleford Elizabeth , Barjaktarevic Igor , Basta Patricia , Clish Clary B. , Comellas Alejandro P. , Cornell Elaine , Curtis Jeffrey L. , Doerschuk Claire , Durda Peter , Emson Claire , Freeman Christine M. , Guo Xiuqing , Hastie Annette T. , Hawkins Gregory A. , Herrera Julio , Johnson W. Craig , Labaki Wassim W. , Liu Yongmei , Masters Brett , Miller Michael , Ortega Victor E. , Papanicolaou George , Peters Stephen , Taylor Kent D. , Rich Stephen S. , Rotter Jerome I. , Auer Paul , Reiner Alex P. , Tracy Russell P. , Ngo Debby , Gerszten Robert E. , O'Neal Wanda K. , Bowler Russell P. ,   NHLBI Trans‐Omics for Precision Medicine (TOPMed) Consortium
Published: May 2020

AbstractNovel proteomics platforms, such as the aptamer‐based SOMAscan platform, can quantify large numbers of proteins efficiently and cost‐effectively and are rapidly growing in popularity. However, comparisons to conventional immunoassays remain underexplored, leaving investigators unsure when cross‐assay comparisons are appropriate. The correlation of results from immunoassays with relative protein quantification is explored by SOMAscan. For 63 proteins assessed in two chronic obstructive pulmonary disease (COPD) cohorts, subpopulations and intermediate outcome measures in COPD Study (SPIROMICS), and COPDGene, using myriad rules based medicine multiplex immunoassays and SOMAscan, Spearman correlation coefficients range from −0.13 to 0.97, with a median correlation coefficient of ≈0.5 and consistent results across cohorts. A similar range is observed for immunoassays in the population‐based Multi‐Ethnic Study of Atherosclerosis and for other assays in COPDGene and SPIROMICS. Comparisons of relative quantification from the antibody‐based Olink platform and SOMAscan in a small cohort of myocardial infarction patients also show a wide correlation range. Finally, cis pQTL data, mass spectrometry aptamer confirmation, and other publicly available data are integrated to assess relationships with observed correlations. Correlation between proteomics assays shows a wide range and should be carefully considered when comparing and meta‐analyzing proteomics data across assays and studies.
Characterization of the Myofibrillar Proteome as a Way to Better Understand Differences in Bovine Meats Having Different Ultimate pH Values
Authors: Fuente‐Garcia Claudia , Sentandreu Enrique , Aldai Noelia , Oliván Mamen , Sentandreu Miguel Ángel 
Published: May 2020

AbstractInfluence of ultimate pH (pHu) on the occurrence of defective meats known as dark, firm, and dry (DFD) meats has been studied through a proteomic approach at early post‐mortem times. The myofibrillar sub‐proteome of longissimus thoracis et lumborum muscle from twelve loin samples from Asturiana de los Valles x Friesian yearling bulls, previously classified into two groups of six samples according to their pH values (normal, pHu < 6.0 and high, pHu ≥ 6.0), is analyzed at 24 h post‐mortem. Fractionation/enrichment of muscle samples is carried out by combining OFFGEL fractionation in the pH range 4–7 followed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS‐PAGE) of the retrieved liquid fractions. Four protein bands satisfactorily discriminate between meat samples with normal and high pHu. These bands are quantified by image analysis, and further identified by liquid chromatography‐mass spectrometry as desmin, pyruvate kinase, myosin light chain, and myosin heavy chain‐1 and ‐2. Coupling OFFGEL and SDS‐PAGE separation with MS provides detailed and reproducible myofibrillar protein profiles enabling comparison among the sample groups assayed. This makes feasible to identify biomarkers capable to better understand pre‐slaughter stress condition susceptible to give DFD meats with high pHu values.
Chaperone action of a cofactor in protein folding
Authors: Chen Chen , Park Chiwook 
Published: May 2020

AbstractPreviously, we have reported that ATP accelerates the folding and unfolding of Escherichia coli glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH), which is a glycolytic enzyme utilizing NAD+ as a cofactor. Because ATP and NAD+ share the ADP moiety, we hypothesized that NAD+ also accelerates the folding of GAPDH and that the common structural motif between ATP and NAD+ is responsible for the chaperone activity. After confirming that NAD+ indeed accelerates the folding of GAPDH, we examined the chaperone activity of the structural fragments of NAD+ (ADP, AMP, adenosine, and nicotinamide monophosphate). Our finding showed that ADP and AMP significantly speed up the folding of GAPDH, while adenosine and nicotinamide monophosphate do not. ADP and AMP also dramatically speed up the unfolding of GAPDH by selectively stabilizing a transition state in which GAPDH has a partially unfolded conformation. Similar to the previously reported effect of ATP on the equilibrium unfolding of GAPDH, a partially unfolded intermediate also accumulates in the presence of ADP and AMP. Based on the effect of the structural fragments of NAD+ on the folding of GAPDH, we identified that AMP is the structural determinant of the chaperone activity of ATP and NAD+. Also, we propose a plausible model to explain how NAD+ accelerates the folding of GAPDH through a stepwise development of molecular interactions with the protein.
Identification of environmental factors that influence the likelihood of off‐target movement of dicamba
Authors: Oseland Eric , Bish Mandy , Steckel Larry , Bradley Kevin 
Published: May 2020

AbstractBACKGROUNDCommercialization of dicamba‐resistant soybean and cotton and subsequent post‐emergence applications of dicamba contributed to at least 1.4 and 0.5 million hectares of dicamba‐injured soybean in the United States in 2017 and 2018, respectively. This research was initiated to identify environmental factors that contribute to off‐target dicamba movement. A survey was conducted following the 2017 growing season to collect information from dicamba applications that remained on the target field and those where dicamba moved. Weather and environmental data surrounding applications were collected and used to identify factors that reduce the likelihood of off‐target movement. Soil pH was one factor identified in the model, and field experiments were conducted in 2018 and 2019 to validate the model. Three commercially‐available dicamba formulations and one formulation currently in development were applied to soil at five distinct pH values. Sensitive soybean was used as a bioassay plant to detect dicamba volatilization.RESULTSWind speeds the day of and following application, nearest water source to the field, soybean production acreage in the county, and soil pH were identified as factors that influence the likelihood for off‐target movement. In the field study, when dicamba was applied to pH‐adjusted soil and placed under low tunnels for 72 h, dicamba volatility increased when soil pH decreased as the model predicted. Dicamba choline, which is not commercially available, had reduced volatility compared to other formulations tested.CONCLUSIONResults of this study identified specific factors that contribute to successful and unsuccessful dicamba applications and should be considered prior to applications. © 2020 Society of Chemical Industry
Effect of fenugreek consumption on serum lipid profile: A systematic review and meta‐analysis
Authors: Heshmat‐Ghahdarijani Kiyan , Mashayekhiasl Neda , Amerizadeh Atefeh , Teimouri Jervekani Zahra , Sadeghi Masoumeh 
Published: May 2020

AbstractVarious studies have shown that Trigonella foenum‐graecum (fenugreek) supplementation has lipid‐lowering activity. This meta‐analysis was performed to evaluate the effect of fenugreek supplementation on human serum lipid profile. Data sources were PubMed, EMBASE, Scopus, and Coherence library which were searched systematically from January 2000 up to December 2019. Inclusion criteria were randomized clinical trial (RCT) study design, at least one of lipid profile components (triglyceride [TG], total cholesterol [TC], low‐density lipoprotein cholesterol, and high‐density lipoprotein cholesterol) levels measured before fenugreek use and one of the lipid components level reported as result. The pooled weighted mean difference (MD) and its 95% confidence interval (CI) were calculated and pooled using a random‐effect model. Only articles published in English were considered. Fifteen RCTs involving 281 cases consumed fenugreek and 255 control cases in controlled group (11 articles) and 136 cases in uncontrolled group (4 articles) were analyzed in our study. Pooled data of indicated a significant impact of fenugreek supplementation on lowering TC (−1.13 [−1.88, −0.37]; p = .003), low‐density lipoprotein (LDL) (−1.26 [−2.09, −0.43]; p = .003), and TG (−1.07 [−1.82, −0.33]; p = 0.005) and increasing the high‐density lipoprotein (HDL) level (0.70 [0.07, 1.34]; p = .03) compared with the control group. There were no significant differences in TC, TG, and LDL between pre‐ and post‐fenugreek studies in the noncontrolled studies however, the result of combination of four studies without control group showed a significant increase in mean HDL (0.81 [0.33,1.29]; p‐value = .001). The results of subgroup analysis showed that the fenugreek reduced the TG and LDL and increases HDL levels in diabetic subjects more effectively. Fenugreek supplementation significantly improved lipid profile (LDL, TG, TC, and HDL). It could be considered as an effective lipid‐lowering medicinal plant. Further high‐quality studies are needed to firmly establish the clinical efficacy of the plant.
Processable N‐Substituted Polybenzimidazole; Direct Synthesis
Authors: Olvera‐Mancilla Jessica , Aguilar‐Lugo Carla , Fernández‐Gijón César Augusto , Palacios‐Alquisira Joaquín , Zolotukhin Mikhail , Alexandrova Larissa 
Published: May 2020

AbstractIn this work, the preparations of N‐phenyl substituted polybenzimidazoles by direct polycondensation between N‐phenyl substituted aromatic tetramine, 4,6‐di(N‐phenylamine)‐1,3‐diaminebenzene, and dicarboxylic acids in Eaton's reagent are reported. It is shown that the factors of concentration and temperature are very important for synthesis of soluble linear polymer structures. The high temperatures promote strong crosslinking reactions resulting in insoluble gel fraction. Only decreasing the reaction temperature to 90 °C it is possible to obtained soluble polymer of high molecular weights with inherent viscosity of 1.6 dL/g. Although the FTIR and 1H NMR analyses demonstrated incomplete cyclization of thus obtained polymers, but they are readily soluble in organic solvents and the good quality films were prepared by solution casting. In order to complete the cyclization films were undergone by thermal treatment at 320 °C during 1 h. The N‐phenyl substituted polybenzimidazoles demonstrated good thermal and mechanical properties, which were compared to their analog unsubstituted polybenzimidazoles obtained from 1,2,4,5‐Benzenetetramine tetra‐hydrochloride using similar synthetic conditions.
Synthesis, Spectroscopic Characterizations of Novel Norcantharimides, Their ADME Properties and Docking Studies Against COVID‐19 Mpr°
Authors: Özkan Hamdi , Adem Şevki 
Published: May 2020

AbstractA series of novel Norcantharimide derivatives were synthesized and their structures were characterized by FTIR, 1H and 13C NMR spectroscopy as well as elemental analyses. The absorption, distribution, metabolism and excretion (ADME) properties of the synthesized molecules were investigated. The results obtained in silico demonstrated that these molecules can be considered as orally active drug candidates due to their physicochemical properties. Also, docking studies demonstrated that all derivatives exhibit a good theoretical affinity with MolDock Score in between 124–138 against the main protease of Coronavirus Disease 2019 (COVID‐19 Mpr°) that caused worldwide epidemics. We believe that newly synthesized norcantharimide derivatives can guide many future studies in organic synthesis, medicine and pharmaceutical applications.
Low‐Temperature Synthesis of a Sulfur‐Polyacrylonitrile Composite Cathode for Lithium‐Sulfur Batteries
Authors: Páez Jerez Ana L. , Chemes Doly M. , Sham Edgardo L. , Davies Lilian E. , Tesio Alvaro Y. , Flexer Victoria 
Published: May 2020

AbstractThrough a novel synthesis process, a novel sulfurized polyacrylonitrile (SPAN) composite, noted as DH‐150‐300‐SPAN, was prepared and tested as cathode material for lithium sulfur (Li−S) batteries. The reported process involves two heating steps at low temperature. The first plateau at 150 °C melts elemental sulfur, while the second, at 300 °C, produces the cycling of polyacrylonitrile, and incorporates sulfur to the cyclic polymeric structure. A fully morphological and electrochemical characterization was carried out. The SPAN composite contains (38.3 ± 0.2) % S. Li−S batteries assembled using these cathodes have shown good performance, reaching high and stable capacity values, above 1000 mAh
after 150 cycles at 0.1 C. This is the first time that stable cycling is achieved for a SPAN composite synthesized at such low temperatures and for short heating treatments. Our work describes an easy route to prepare a promising candidate for cathode material of Li−S batteries by using a low‐cost process and low‐cost materials.
The influence of casting position and disturbance induced by reinforcement on the structural concrete strength
Authors: Moccia Francesco , Kubski Xavier , Fernández Ruiz Miguel , Muttoni Aurelio 
Published: May 2020

AbstractIt is well known that control specimens used to assess the concrete strength of new structures have different casting and curing conditions than those of actual structures. Notably, after pouring of the concrete and before its hardening, a number of phenomena such as concrete bleeding and plastic settlement occur, influencing the in‐situ strength with respect to that of small and homogeneous control specimens (cubes or cylinders). In addition, the development of these phenomena and their structural implications are influenced by the presence of reinforcing bars, disturbing the settlement and bleeding of fresh concrete. In this paper, these aspects, with particular emphasis on the effective structural strength, are investigated by means of a testing program performed with refined measurement techniques such as tomography and Digital Image Correlation. On that basis, consistent design rules are derived to correct the strength of control specimens in order to calculate the resistance of a structural concrete member.
Neotypification of Manettia reclinata (Rubiaceae)
Authors: Kirkbride Joseph H. , Wiersema John H. 
Published: May 2020

AbstractThe nomenclature of neotropical Manettia reclinata L., type of the genus Manettia Mutis, is reviewed. Without access to a specimen or illustration, Linnaeus compiled the descriptions of the genus and species entirely from correspondence received from Mutis in present‐day Colombia; thus, no original material exists, and application of the name can only be fixed by neotypification. An earlier attempt to neotypify this name on a Brazilian collection was not effectively published. Therefore, an image of this taxon prepared during the Real Expedición Botánica del Nuevo Reino de Granada under the direction of Mutis is more appropriately designated as neotype.
Rigorous analysis of Arnol'd tongues from a manifold piecewise linear circuit
Authors: Le Viet Duc , Tsubone Tadashi , Inaba Naohiko 
Published: May 2020

AbstractThis report presents rigorous analysis of Arnol'd tongues generated by a manifold piecewise linear circuit. The circuit comprises an LC oscillator, a conductive switch that produces the manifold, and a voltage source controlled by the current value through the inductor when the voltage across the capacitor exceeds a threshold. From the circuit dynamics, the Poincaré return map is explicitly derived as a piecewise linear circle map, and the generation of Arnol'd tongues is precisely explained. We attempt to derive the border–collision bifurcation boundaries of the Arnol'd tongues, some of which are obtained explicitly. Furthermore, we experimentally verify these theoretical results. © 2020 Institute of Electrical Engineers of Japan. Published by John Wiley & Sons, Inc.
Doppler‐based predictive model for methotrexate resistance in low‐risk gestational trophoblastic neoplasia with myometrial invasion: prospective study of 147 patients
Authors: Qin J. , Zhang S. , Poon L. , Pan Z. , Luo J. , Yu N. , Wang L. , Wu X. , Cheng X. , Xie X. , Lu Y. , LU W. 
Published: May 2020

ABSTRACTObjectivesThis prospective clinical study aimed to evaluate the vascularization characteristics of low‐risk gestational trophoblastic neoplasia (GTN) using Doppler imaging and to develop a predictive model for resistance to methotrexate (MTX).MethodsPatients with low‐risk GTN receiving primary MTX treatment were enrolled from the Women's Hospital, Zhejiang University School of Medicine, Hangzhou, China, from September 2012 to August 2018. The primary endpoint was to develop and internally validate a predictive model for resistance to MTX therapy in these patients. In the training set, clinical features and Doppler hemodynamic parameters before MTX therapy were analyzed using logistic regression to identify independent predictors of MTX resistance, which were integrated into the model. The predictive performance of the model was evaluated by leave‐one‐out cross‐validation in the training dataset and internal validation in an independent‐sample test dataset.ResultsThe entire imaging protocol was completed by 147 eligible patients, of which 110 comprised the training set and 37 the test set. In the training set, cases with myometrial invasion (81.8%; 90/110) showed vascular‐enriched areas in the myometrium and high velocity and low impedance ratios of the uterine artery (UtA) compared to cases without myometrial invasion (18.2%; 20/110). On multivariate logistic regression analysis, time‐averaged mean velocity in UtA (UtA‐TAmean) and the International Federation of Gynecology and Obstetrics (FIGO) score were identified as independent predictors (P = 0.009 and P = 0.043, respectively) of MTX resistance. The Doppler‐based predictive model, developed based on the 90 cases with myometrial invasion, was y = −2.95332 + 0.41696 × FIGO score + 0.03551 × UtA‐TAmean. The model showed an area under the curve of 0.757 (95% CI, 0.653–0.862) and the optimal cut‐off value was 0.50622, which had 45.2% sensitivity and 96.6% specificity. The model stratified patients with low‐risk GTN into low (< 10%), intermediate (10–90%) and high (> 90%) probability of MTX resistance, based on the threshold values of −1.59544 and 0.10046. The model had an accuracy of 74.4% (95% CI, 64.5–82.3%) in the cross‐validation and 72.7% (95% CI, 55.8–84.9%) in the internal validation.ConclusionsThe Doppler‐based predictive model, combining a non‐invasive marker of tumor vascularity with the FIGO scoring system, can differentiate cases with low from those with high probability of developing MTX resistance and therefore has the potential to guide treatment options in patients with low‐risk GTN and myometrial invasion. © 2020 Authors. Ultrasound in Obstetrics & Gynecology published by John Wiley & Sons Ltd on behalf of International Society of Ultrasound in Obstetrics and Gynecology.
Detection of human‐associated bacteria in water from Akiyoshi‐do Cave, Japan
Authors: Ando Kanato , Murakami Takashi 
Published: May 2020

AbstractAkiyoshi‐do Cave is one of the oldest and the largest show caves in Japan. Environmental alterations induced by tourism impacts have been suggested; however, only a few previous studies have investigated the impacts of tourism on the cave. In this study, enrichment culture procedures were applied to detect human‐associated bacteria (HAB) including Escherichia coli, Staphylococcus aureus, and thermo‐tolerant Bacillus spp. in cave water. Physical and bacterial parameters of water including total nitrogen, ammonia, phosphate, total number of bacteria, and total number of viable bacteria were collected as environmental factors. Escherichia coli was absent at all sites, but increased levels of total bacteria, viable bacteria, S. aureus, and thermo‐tolerant Bacillus spp. were present at high‐impact sites. Examination of the origin tracking of HAB suggested that cave tourists could be a source of HAB contamination, but other causes related to the surface land use could also contribute to HAB contamination. © 2020 Water Environment FederationPractitioner points

Detection of HAB was performed for the first time in Japanese show caves to consider tourism impacts.

The greater number of HAB was detected from the tourist area than the nontourist area in the cave.

It was suggested that the origin of HAB may not be limited to tourists, but may also include the surface.
Low-dose radiation therapy for COVID-19 pneumopathy: what is the evidence?
Authors: Rödel Franz , Arenas Meritxell , Ott Oliver J. , Fournier Claudia , Georgakilas Alexandros G. , Tapio Soile , Trott Klaus-Rüdiger , Gaipl Udo S. 
Published: May 2020

AbstractIn the current dismal situation of the COVID-19 pandemic, effective management of patients with pneumonia and acute respiratory distress syndrome is of vital importance. Due to the current lack of effective pharmacological concepts, this situation has caused interest in (re)considering historical reports on the treatment of patients with low-dose radiation therapy for pneumonia. Although these historical reports are of low-level evidence per se, hampering recommendations for decision-making in the clinical setting, they indicate effectiveness in the dose range between 0.3 and 1 Gy, similar to more recent dose concepts in the treatment of acute and chronic inflammatory/degenerative benign diseases with, e.g., a single dose per fraction of 0.5 Gy. This concise review aims to critically review the evidence for low-dose radiation treatment of COVID-19 pneumopathy and discuss whether it is worth investigating in the present clinical situation.
Kostenfaktor „ambulante Wundversorgung“ in der Notaufnahme
Authors: Schindler Cora R. , Lustenberger Thomas , Marzi Ingo , Verboket René D. 
Published: May 2020

Zusammenfassung
Hintergrund
Die Erstversorgung von Wunden und kleinere chirurgische Eingriffe gehören neben der hochspezialisierten Medizin zu den allgemein notwendigen Grundleistungen der Notfallversorgung in den Kliniken. Die Vergütung der ambulanten Notfallleistungen für gesetzlich Versicherte erfolgt derzeit nach dem Einheitlichen Bewertungsmaßstab (EBM), welchem die betriebswirtschaftliche Aufwandserfassung des niedergelassenen Sektors als Kalkulationsgrundlage dient. Krankenhäuser haben im Vergleich zu Arztpraxen wesentlich höhere Vorhaltungskosten.

Ziel der Arbeit
In dieser Arbeit wird das entstehende Kosten-Erlös-Verhältnis der ambulanten Wundversorgung in einer Notaufnahme durch die Vergütung nach EBM analysiert.

Material und Methoden
Die Daten wurden in der Notaufnahme des Universitätsklinikums Frankfurt am Main über 12 Monate erhoben. Eingeschlossen wurden alle Patienten, die in diesem Zeitraum eine Wundversorgung mittels Naht erhielten. Die Kosten wurden der Abrechnung nach EBM 01210 (bzw. 01212) mit der Zusatzpauschale für kleinchirurgische Eingriffe EBM 02301 gegenübergestellt.

Ergebnisse
Im Beobachtungszeitraum wurden 1548 Patienten versorgt; das entspricht 19,52 % aller unfallchirurgischen Fälle. Den Kosten einer Standardwundversorgung in Höhe von 45,40 € steht eine Vergütung von 31,83 € gegenüber. Die Berechnung des Gesamterlöses weist einen Defizitbetrag von 13,57 € pro ambulantem Fall auf; dies entspricht einem Jahresdefizit von 21.006,36 €.

Diskussion
Es konnte gezeigt werden, dass ohne Betrachtung der relevanten Vorhaltekosten in keinem Fall eine Kostendeckung erreicht werden kann.
Die bisherige Vergütung der ambulanten Wundversorgung nach EBM erscheint unzureichend. Eine Anpassung bzw. Zusatzvergütung scheint notwendig, um eine ausreichende Versorgungsqualität in Zukunft sicherstellen zu können.