bluuebunny/crossref_metadata_2025_split huggingface dataset:
Gradient based first- and second-order filters for the demarcation of continental–oceanic boundaries using satellite gravity data
Authors: Maurya Ved P , Fontes Sergio L , Oliveira Jr Vanderlei C , La Terra Emanuele F 
Published: February 2020

SUMMARY
Demarcating the continental–oceanic boundary (COB) is extremely important as it contributes to an understanding of the tectonic environments of marginal basins through plate reconstructions. In general, the COB is estimated by jointly interpreting the features of the basement geomorphology, bathymetry and geophysical datasets, including gravity, magnetic, seismic and geochemical data. We propose a novel methodology named the tilt-theta angles correlation (TTAC), a second-order filtering approach to estimate the COB using high-resolution satellite gravity data. This method computes the angular difference or correlation angle between the vectors computed from the gradients of the tilt and theta derivative-based first-order filters. Oceanic regions generally exhibit small correlation angles (∼0°), thereby indicating a direct correlation; in contrast, continental regions depict large correlation angles (∼180°) and thus display an inverse correlation. The TTAC method marks the COB at the spatial position where the correlation angles abruptly shift from inverse to direct. Tests using synthetic data and the larger spectral energy content of TTAC for long wavelengths over tilt, theta and minus of the sign of vertical derivative (-SiVD) filters attest the effectiveness of the new methodology over first-order derivative based filters. Additionally, tests utilizing real data over both the West African Transform Margin (WATM) and across the South Atlantic Conjugate Margin (SACM) provide favourable results compared with the previous methodologies used to demarcate the COB. The COB estimates derived using the new methodology are consistent with the COB estimates obtained during recent plate-reconstruction studies. We also compare our results with an earlier interpretation along a conjugate margin encompassing magma-poor and magma-rich regions. In the complex rifted environments of the SACM, in which volcanic rocks are dominant, the TTAC methodology substantially improves the previous COB estimates, thereby establishing it as an efficient edge marker by simultaneously reducing small-scale geological noise and enhancing regional-scale geological contributions.
Adhesion of freshwater sponge cells mediated by carbohydrate–carbohydrate interactions requires low environmental calcium
Authors: Vilanova Eduardo , Ciodaro Priscilla J , Bezerra Francisco F , Santos Gustavo R C , Valle-Delgado Juan J , Anselmetti Dario , Fernàndez-Busquets Xavier , Mourão Paulo A S 
Published: February 2020

AbstractMarine ancestors of freshwater sponges had to undergo a series of physiological adaptations to colonize harsh and heterogeneous limnic environments. Besides reduced salinity, river-lake systems also have calcium concentrations far lower than seawater. Cell adhesion in sponges is mediated by calcium-dependent multivalent self-interactions of sulfated polysaccharide components of membrane-bound proteoglycans named aggregation factors. Cells of marine sponges require seawater average calcium concentration (10 mM) to sustain adhesion promoted by aggregation factors. We demonstrate here that the freshwater sponge Spongilla alba can thrive in a calcium-poor aquatic environment and that their cells are able to aggregate and form primmorphs with calcium concentrations 40-fold lower than that required by marine sponges cells. We also find that their gemmules need calcium and other micronutrients to hatch and generate new sponges. The sulfated polysaccharide purified from S. alba has sulfate content and molecular size notably lower than those from marine sponges. Nuclear magnetic resonance analyses indicated that it is composed of a central backbone of non- and 2-sulfated α- and β-glucose units decorated with branches of α-glucose. Assessments with atomic force microscopy/single-molecule force spectroscopy show that S. alba glucan requires 10-fold less calcium than sulfated polysaccharides from marine sponges to self-interact efficiently. Such an ability to retain multicellular morphology with low environmental calcium must have been a crucial evolutionary step for freshwater sponges to successfully colonize inland waters.
Impact of the ‘Seguro Médico Siglo XXI’ medical insurance programme on neonatal and infant mortality in Mexico, 2006–14: an ecological approach to estimation
Authors: Urquieta-Salomón José , Lamadrid-Figueroa Héctor , Angeles Gustavo , Montoya Alejandra , Rojas-Martínez Rosalba , Martínez-Nolasco Alejandro , Torres-Pereda Pilar , O’Shea Gabriel , Villagrán Victor M , Halley Elizabeth , Delgado-Sánchez Verónica , Lazcano-Ponce Eduardo 
Published: February 2020

Abstract
The ‘Seguro Médico Siglo XXI’ (SMSXXI), a universal coverage medical insurance programme for children under 5 years of age, started in 2006 to help avoid catastrophic health expenditures in poor families without social security in Mexico. The study used information from the National Health Information System for the 2006–14 period. An ecological approach was followed with a panel of the 2457 municipalities of Mexico as the units of analysis. The outcome variables were the municipality-level neonatal mortality and infant mortality rates in population without access to social security. The programme variable was the coverage of the SMSXXI programme at the municipality level, expressed as a proportion. Demographic and economic variables defined at the municipality level were included as covariates. Impact was estimated by fitting a fixed-effects negative binomial regression model. Results reveal that the SMSXXI significantly reduced both infant and neonatSal mortality in the target population, although in a non-linear fashion, with minimum mortality levels found around the 70% coverage range. The effect is mostly given by the transition from the first quintile to the fourth quintile of coverage (&lt;13% vs 70.5–93.7% coverage), and it is attenuated significantly at coverage levels very close to or at 100%. The observed risk reduction amounted to an estimated total of 11 358 infant deaths being avoided due to the SMSXXI during the 2006–14 period, of which 48% were neonatal. In conclusion, we found a significant impact of the SMSXXI programme on both infant mortality and neonatal mortality. An attenuation of the effect of the insurance on mortality rates at levels close to 100% coverage may reflect the saturation of health units in detriment of the quality of care.
CHCHD2 harboring Parkinson’s disease-linked T61I mutation precipitates inside mitochondria and induces precipitation of wild-type CHCHD2
Authors: Cornelissen Tom , Spinazzi Marco , Martin Shaun , Imberechts Dorien , Vangheluwe Peter , Bird Matthew , De Strooper Bart , Vandenberghe Wim 
Published: February 2020

Abstract
The T61I mutation in coiled-coil-helix-coiled-coil-helix domain containing 2 (CHCHD2), a protein residing in the mitochondrial intermembrane space (IMS), causes an autosomal dominant form of Parkinson’s disease (PD), but the underlying pathogenic mechanisms are not well understood. Here, we compared the subcellular localization and solubility of wild-type (WT) and T61I mutant CHCHD2 in human cells. We found that mitochondrial targeting of both WT and T61I CHCHD2 depended on the four cysteine residues in the C-terminal coiled-coil-helix-coiled-coil-helix (CHCH) domain but not on the N-terminal predicted mitochondrial targeting sequence. The T61I mutation did not interfere with mitochondrial targeting of the mutant protein but induced its precipitation in the IMS. Moreover, T61I CHCHD2 induced increased mitochondrial production of reactive oxygen species and apoptosis, which was prevented by treatment with anti-oxidants. Retention of T61I CHCHD2 in the cytosol through mutation of the cysteine residues in the CHCH domain prevented its precipitation as well as its apoptosis-inducing effect. Importantly, T61I CHCHD2 potently impaired the solubility of WT CHCHD2. In conclusion, our data show that the T61I mutation renders mutant CHCHD2 insoluble inside mitochondria, suggesting loss of function of the mutant protein. In addition, T61I CHCHD2 exerts a dominant-negative effect on the solubility of WT CHCHD2, explaining the dominant inheritance of this form of PD.
The Contribution of Cognitive Linguistics to the Acquisition of Polysemy: A Dictionary Entry-Based Study with Chinese Learners of English
Authors: Lu Huaguo , Zhang Ya , Hao Xia 
Published: February 2020

AbstractThis paper reported on a quasi-experimental study into the effectiveness of two types of entries – entries enhanced with cognitive linguistic insights (CL entries) and linearly ordered entries (LO entries) – in promoting L2 learners’ acquisition of English polysemes. Sixty-five college students majoring in English were divided into an experimental group (EG) and a reference group (RG). EG received the CL entries for lift and break in and RG were presented with the corresponding LO entries. Both groups were given twenty minutes to read the entries and to learn the twenty-seven senses. To examine the effectiveness of the two types of entries, an English-Chinese translation test was administered before the learning session (as Test 1), immediately after it (as Test 2) and two weeks later (as Test 3). The results showed that: (1) EG outperformed RG in the comprehension of the target senses on both Test 2 and Test 3. (2) The CL entries were more effective in promoting short-term retention than the LO entries, but had no advantage over the latter in long-term retention. (3) The CL entry for lift facilitated the learning of the target senses more than the LO entry did, but the CL entry for break in was no more effective than the LO entry in helping L2 learners acquire the target senses. The study suggests that the cognitive linguistic view can and should be further applied to treating polysemy in learner’s dictionaries, but the model for CL entries is not always recommendable and the knowledge gained from reading the CL entries needs consolidating.
Postvaccination Serum Antirotavirus Immunoglobulin A as a Correlate of Protection Against Rotavirus Gastroenteritis Across Settings
Authors: Baker Julia M , Tate Jacqueline E , Leon Juan , Haber Michael J , Pitzer Virginia E , Lopman Benjamin A 
Published: February 2020

Abstract

Background
A correlate of protection for rotavirus gastroenteritis would facilitate rapid assessment of vaccination strategies and the next generation of rotavirus vaccines. We aimed to quantify a threshold of postvaccine serum antirotavirus immunoglobulin A (IgA) as an individual-level immune correlate of protection against rotavirus gastroenteritis.

Methods
Individual-level data on 5074 infants in 9 GlaxoSmithKline Rotarix Phase 2/3 clinical trials from 16 countries were pooled. Cox proportional hazard models were fit to estimate hazard ratios (HRs) describing the relationship between IgA thresholds and occurrence of rotavirus gastroenteritis.

Results
Seroconversion (IgA ≥ 20 U/mL) conferred substantial protection against any and severe rotavirus gastroenteritis to age 1 year. In low child mortality settings, seroconversion provided near perfect protection against severe rotavirus gastroenteritis (HR, 0.04; 95% confidence interval [CI], .01–.31). In high child mortality settings, seroconversion dramatically reduced the risk of severe rotavirus gastroenteritis (HR, 0.46; 95% CI, .25–.86). As IgA threshold increased, risk of rotavirus gastroenteritis generally decreased. A given IgA threshold provided better protection in low compared to high child mortality settings.

Discussion
Postvaccination antirotavirus IgA is a valuable correlate of protection against rotavirus gastroenteritis to age 1 year. Seroconversion provides an informative threshold for assessing rotavirus vaccine performance.
Thinking outside the Box: Globalization, Labor Rights, and the Making of Preferential Trade Agreements
Authors: Wang Zhiyuan 
Published: February 2020

AbstractStates can seemingly defy the dictates of globalization. In practice, although being pressured by their competitors, states rarely engage in the race to the bottom by downgrading labor rights laws that are politically costly to pursue. I argue that states’ resistance is made possible by adopting more viable policy alternatives, i.e., concluding preferential trade agreements (PTAs). PTAs can generate considerable economic gains in a less politically costly way than does reducing legal labor protection. As a result, it is expected that a pair of states is more likely to form a PTA in the face of policy pressure to lower legal labor protection. I also argue that facing such pressure, these states are more likely to include strong labor provisions in PTAs. Finally, in the face of the policy pressure, states may feel that signing a PTA is a bit less urgent when they are able to diminish practical labor protection. Applying structural equivalence technique to a new global labor rights dataset to capture the policy pressure to lower legal labor protection, I find robust evidence in support of these conjectures.
Inactivation of thymidine kinase as a cause of resistance to zidovudine in clinical isolates of Escherichia coli: a phenotypic and genomic study
Authors: Peyclit Lucie , Ben Khedher Maryem , Zerrouki Lotfi , Diene Seydina M , Baron Sophie Alexandra , Rolain Jean-Marc 
Published: February 2020

AbstractObjectivesThe antiviral zidovudine has been recently identified as an active drug against resistant Enterobacteriaceae, but prevalence of resistance to this compound remains unknown. The aim was to estimate the prevalence of clinical Escherichia coli isolates resistant to zidovudine and to decipher the mechanism of zidovudine resistance.MethodsWe screened 537 isolates on zidovudine-containing agar plates and studied their thymidine kinase (tdk) gene sequences, the putative target involved in zidovudine resistance. Moreover, sequence analysis of 633 complete genomes of E. coli was performed to investigate mutation in the tdk gene. A comparative genomic analysis was done on an in vitro zidovudine-resistant mutant.ResultsAfter screening on our medium containing 2.7 mg/L (10 μM) zidovudine, nine strains had a zidovudine MIC &gt;26.7 mg/L. The gene was absent in three isolates, inactivated by an IS (IS1X2 and ISApl1) in two isolates and mutated in four isolates. A genomic analysis of 633 E. coli genomes showed heterogeneity of the tdk gene sequence, with 27 different sequences. Among them, three genomes showed an inactivation of the gene (IS, stop codon and no tdk gene sequence). The in vitro mutant E. coli had 27 SNPs in eight genes of the core genome compared with the initial strain.ConclusionsOur study reports zidovudine-resistant clinical isolates of E. coli, presumably related to tdk inactivation. Diversity of Tdk in bacterial genomes can be large. Other mechanisms need to be considered in zidovudine resistance. The use of zidovudine in antibiotic-resistant infections needs to be in combination and should be tested before clinical administration.
BL30SEC Method for Detection of Beta-Lactams in Raw Commingled Cow Milk: AOAC Performance Tested MethodSM 061902
Authors: Markovsky Robert J , Douglas David W , Sullivan Ryan , Tran Alan C , Legg David R , McRobbie Lindsey W , Schwartz Janine A , Salter Robert S , Klass Nicole , Knoop Sarah , Bastin Benjamin , Crowley Erin , Agin James , Goins David , Kalinowsky Emily 
Published: February 2020

Abstract
Testing milk for antibiotics before acceptance into dairies is required by the U.S. Pasteurized Milk Ordinance. Technological advances in tests have reduced screening times and improved detection accuracy. This work describes the validation of the Charm Rapid One Step Assay Beta-Lactam 30 Second Test according to the U.S. Food and Drug Administration Center for Veterinary Medicine protocol for raw commingled milk. Milk is added to the lateral flow test strip in an incubator/reader to deliver a 30 second result. Independent laboratory validation followed sensitivity, interference, and incurred residue protocols. Sensitivity, in parts per billion (ppb = µg/kg), using a probit curve determined 90% percent detection with 95% confidence, which met National Conference of Interstate Milk Shipments (NCIMS) specifications. Six U.S. approved beta-lactam drugs were detected below, but within 50% of, target/tolerance levels for penicillin G 2.9 ppb, ampicillin 5.9 ppb, amoxicillin 5.8 ppb, cephapirin 13 ppb, cloxacillin 8.1 ppb, and ceftiofur metabolites 73 ppb. No interferences were observed from 33 animal drugs at 100 ppb, somatic cells at 1.2 million/mL, or bacterial levels of &gt;300 000 CFU/mL. Incurred residue detection levels were similar to levels determined with the spiked parent compound. The data support NCIMS that the BL30SEC method met U.S. criteria for testing milk for beta-lactams.
Expansion of the Distribution of Aedes albopictus (Diptera: Culicidae): New Records in Northern Argentina and Their Implications From an Epidemiological Perspective
Authors: Goenaga S , Chuchuy A , Micieli M V , Natalini B , Kuruc J , Kowalewski M 
Published: February 2020

Abstract
Since the last yellow fever (YF) outbreak was detected in Argentina in 2009, vector surveillance and studies of arbovirus infections are carried out intermittently specifically in areas where nonhuman primates of the Alouatta genus are present. We report in these areas of Corrientes province the detection of Haemagogus leucocelaenus (Dyar and Shannon) (Diptera: Culicidae) and Sabethes albiprivus (Theobald) (Diptera: Culicidae), both species involved in the forest YF cycle, and also the presence of Aedes albopictus (Skuse) in new areas in Argentina, which represents the southernmost citation for this species in South America. Aedes albopictus, a mosquito species native to Asia, was reported for the first time in Argentina in 1998, in Misiones province. Since then, no other report has indicated the extension of the distribution of this mosquito. This report shows the importance of performing continual entomological and arboviruses surveillance and highlights the impact that could result from the expansion of Ae. albopictus across Argentina.
Characterization of the Volatiles’ Profiles of the Eggs of Forensically Relevant Lucilia sericata and Phormia regina (Diptera: Calliphoridae) Blow Flies by SPME-Facilitated GC-MS
Authors: Giffen-Lemieux Justine E , Okuda Koji , Rosati Jennifer Y , Musah Rabi A 
Published: February 2020

Abstract
The attraction of necrophagous insects, particularly blow flies, to corpses and carrion is of ecological, economic, and agricultural importance, although the mechanisms by which it occurs are not well understood. Much of the published research on blow fly attractants has focused on volatiles emitted from carrion surrogates, but little attention has been given to the possibility that blow fly eggs themselves may emit chemical cues that are responsible for conspecific and heterospecific insect attraction. In this study, the headspace volatiles emitted from eggs representing two aggregated oviposition events that were collected 1 mo apart from two species of the Calliphoridae family (Order: Diptera), Lucilia sericata (Meigen), and Phormia regina (Meigen) were analyzed via solid-phase microextraction-facilitated GC-MS. The volatiles’ profiles were found to be consistent between samples representing the same species, but unique between the two species. Over 100 molecules covering a wide range of compound classes that included alcohols, aldehydes, esters, amines, ketones, and organosulfur compounds were identified. The profile of volatiles emitted from the L. sericata eggs contained several alkanes and aldehydes, whereas salient features of the P. regina headspace included numerous esters and ketones. Between the two species, 42 compounds were shared, several of which were carboxylic acids. Little overlap between the range of compounds detected and those reported to be emitted from decomposing remains was observed.
Immunohistochemical and nanoString-Based Subgrouping of Clinical Medulloblastoma Samples
Authors: D’Arcy Colleen E , Nobre Liana Figueiredo , Arnaldo Anthony , Ramaswamy Vijay , Taylor Michael D , Naz-Hazrati Lili , Hawkins Cynthia E 
Published: February 2020

Abstract
The diagnosis of medulloblastoma incorporates the histologic and molecular subclassification of clinical medulloblastoma samples into wingless (WNT)-activated, sonic hedgehog (SHH)-activated, group 3 and group 4 subgroups. Accurate medulloblastoma subclassification has important prognostic and treatment implications. Immunohistochemistry (IHC)-based and nanoString-based subgrouping methodologies have been independently described as options for medulloblastoma subgrouping, however have not previously been directly compared. We describe our experience with nanoString-based subgrouping in a clinical setting and compare this with our IHC-based results. Study materials included FFPE tissue from 160 medulloblastomas. Clinical data and tumor histology were reviewed. Immunohistochemical-based subgrouping using β-catenin, filamin A and p53 antibodies and nanoString-based gene expression profiling were performed. The sensitivity and specificity of IHC-based subgrouping of WNT and SHH-activated medulloblastomas was 91.5% and 99.54%, respectively. Filamin A immunopositivity highly correlated with SHH/WNT-activated subgroups (sensitivity 100%, specificity 92.7%, p &lt; 0.001). Nuclear β-catenin immunopositivity had a sensitivity of 76.2% and specificity of 99.23% for detection of WNT-activated tumors. Approximately 23.8% of WNT cases would have been missed using an IHC-based subgrouping method alone. nanoString could confidently predict medulloblastoma subgroup in 93% of cases and could distinguish group 3/4 subgroups in 96.3% of cases. nanoString-based subgrouping allows for a more prognostically useful classification of clinical medulloblastoma samples.
Comparison of Turbidimetric Inhibition Immunoassay, High-Performance Liquid Chromatography, and Capillary Electrophoresis Methods for Glycated Hemoglobin Determination
Authors: Gilani Mehwish , Aamir Mohammad , Akram Ammad , Haroon Zujaja Hina , Ijaz Aamir , Khadim Muhammad Tahir 
Published: February 2020

Abstract

Objective
The purpose of this study was to compare the performances of and evaluate the agreement among glycated hemoglobin values analyzed by using National Glycohemoglobin Standardization Program-certified and International Federation of Clinical Chemistry-standardized analyzers.

This cross-sectional study was conducted at the
Armed Forces Institute of Pathology, Department of Chemical Pathology from March 2019 to May 2019.

Methods
Glycated hemoglobin (HbA1c) was measured in the blood specimens from 100 patients on an ADVIA 1800 by a turbidimetric inhibitory immunoassay (TINIA), Sebia instrument by electrophoresis, and Bio-Rad Variant II Turbo system by high-performance liquid chromatography (HPLC). Quantitative variables were calculated as the mean ± standard deviation (SD). Precision and method comparisons were carried out according to Clinical and Laboratory Standards Institute recommendations. The results obtained from each analyzer were compared by correlation analysis. Method comparison was done by linear regression and Bland-Altman plots using the SPSS software version 24.

Results
The mean ± SD HbA1c values from TINIA, electrophoresis, and HPLC were 7.188% ± 1.89%, 7.164% ± 1.866%, and 7.160% ± 1.85%, respectively. The between-run coefficients of variation for TINIA, electrophoresis, and HPLC were 0.64%, 0.61%, and 0.60%, respectively. All 3 showed good correlation (TINIA, R2 = .994, P = .00; electrophoresis, R2 = .992, P = 0.00; and HPLC, R2 = .994, P = 0.00).

Conclusion
The good clinical agreements of HbA1c and strong correlations between analyzers indicate that these analyzers can be used interchangeably.