bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hybrid liquid biphasic system for cell disruption and simultaneous lipid extraction from microalgae Chlorella sorokiniana CY-1 for biofuel production
Authors: Yew Guo Yong , Chew Kit Wayne , Malek Marlinda Abdul , Ho Yeek-Chia , Chen Wei-Hsin , Ling Tau Chuan , Show Pau Loke 
Published: October 2019

AbstractBackgroundThe extraction of lipids from microalgae requires a pretreatment process to break the cell wall and subsequent extraction processes to obtain the lipids for biofuels production. The multistep operation tends to incur high costs and are energy intensive due to longer process operations. This research work applies the combination of radicals from hydrogen peroxide with an organic solvent as a chemical pretreatment method for disrupting the cell wall of microalgae and simultaneously extracting lipids from the biomass in a one-step biphasic solution.ResultSeveral parameters which can affect the biphasic system were analyzed: contact time, volume of solvent, volume ratio, type of organic solvent, biomass amount and concentration of solvents, to extract the highest amount of lipids from microalgae. The results were optimized and up to 83.5% of lipid recovery yield and 94.6% of enhancement was successfully achieved. The results obtain from GC-FID were similar to the analysis of triglyceride lipid standard.ConclusionThe profound hybrid biphasic system shows great potential to radically disrupt the cell wall of microalgae and instantaneously extract lipids in a single-step approach. The lipids extracted were tested to for its comparability to biodiesel performance.
Whole genome analysis of Black Bengal goat from Savar Goat Farm, Bangladesh
Authors: Chowdhury Shah Md. Ziqrul Haq , Nazir K. H. M. Nazmul Hussain , Hasan Saam , Kabir Ajran , Mahmud Md. Muket , Robbani Mahdi , Tabassum Tahmina , Afroze Tamanna , Rahman Aura , Islam Md. Rafiqul , Hossain Maqsud 
Published: October 2019

Abstract

Objectives
Single nucleotide polymorphisms (SNPs) play critical roles in genetic diversity and disease. Many traits and diseases are linked with exonic SNPs that are significant for gene function, regulation or translation. This study focuses on SNPs that potentially act as the genetic basis for desirable traits in the Black Bengal Goat. This variety of goat is native to South Asia, and is identified as one of the most commercially important meat producing animals in the world. The aim of this study was to sequence the genome of Black Bengal Goats and identify SNPs that might play a significant role in determining meat quality in the organism. The study focuses on exonic SNPs for their greater likelihood of affecting the final translated protein product.

Results
Approximately 76,000 exonic variants were identified in the study. After filtration using a Wilcoxon test based score, the number came down to 49, 965 which were found to be distributed in 11,568 genes. The functional pathways affected by these variations included fatty acid metabolism and degradation, which are important processes that influence meat quality.
Comprehensive anatomic ontologies for lung development: A comparison of alveolar formation and maturation within mouse and human lung
Authors:   On behalf of the Ontology Subcommittee, Pan Huaqin ,   NHLBI Molecular Atlas of Lung Development Program Consortium, Deutsch Gail H. , Wert Susan E. 
Published: October 2019

AbstractBackgroundAlthough the mouse is widely used to model human lung development, function, and disease, our understanding of the molecular mechanisms involved in alveolarization of the peripheral lung is incomplete. Recently, the Molecular Atlas of Lung Development Program (LungMAP) was funded by the National Heart, Lung, and Blood Institute to develop an integrated open access database (known as BREATH) to characterize the molecular and cellular anatomy of the developing lung. To support this effort, we designed detailed anatomic and cellular ontologies describing alveolar formation and maturation in both mouse and human lung.DescriptionWhile the general anatomic organization of the lung is similar for these two species, there are significant variations in the lung’s architectural organization, distribution of connective tissue, and cellular composition along the respiratory tract. Anatomic ontologies for both species were constructed as partonomic hierarchies and organized along the lung’s proximal-distal axis into respiratory, vascular, neural, and immunologic components. Terms for developmental and adult lung structures, tissues, and cells were included, providing comprehensive ontologies for application at varying levels of resolution. Using established scientific resources, multiple rounds of comparison were performed to identify common, analogous, and unique terms that describe the lungs of these two species. Existing biological and biomedical ontologies were examined and cross-referenced to facilitate integration at a later time, while additional terms were drawn from the scientific literature as needed. This comparative approach eliminated redundancy and inconsistent terminology, enabling us to differentiate true anatomic variations between mouse and human lungs. As a result, approximately 300 terms for fetal and postnatal lung structures, tissues, and cells were identified for each species.ConclusionThese ontologies standardize and expand current terminology for fetal and adult lungs, providing a qualitative framework for data annotation, retrieval, and integration across a wide variety of datasets in the BREATH database. To our knowledge, these are the first ontologies designed to include terminology specific for developmental structures in the lung, as well as to compare common anatomic features and variations between mouse and human lungs. These ontologies provide a unique resource for the LungMAP, as well as for the broader scientific community.
Feasibility of radioguided occult lesion localization of clip-marked lymph nodes for tailored axillary treatment in breast cancer patients treated with neoadjuvant systemic therapy
Authors: Hellingman Daan , Donswijk Maarten L. , Winter-Warnars Gonneke A. O. , de Koekkoek-Doll Petra , Pinas Marilyn , Budde-van Namen Yvonne , Westerga Johan , Vrancken Peeters Marie-Jeanne T. F. D. , Kimmings Nikola , Stokkel Marcel P. M. 
Published: October 2019

Abstract

Background
Selective removal of initially tumor-positive axillary lymph nodes in breast cancer patients who underwent neoadjuvant systemic treatment (NST) improves the accuracy of nodal staging and provides the opportunity for more tailored axillary treatment. This study evaluated whether radioguided occult lesion localization (ROLL) of clip-marked lymph nodes is feasible in clinical practice.

Methods
Prior to NST, a clip marker was placed inside a proven tumor-positive lymph node in all breast cancer patients (cTis-4N1-3 M0). After NST, technetium-99m-labeled macroaggregated albumin was injected in the clip-marked lymph nodes. The next day, these ROLL-marked nodes were selectively removed at surgery to evaluate the pathological response of the axilla.

Results
Thirty-seven patients (38 axillae) underwent clip insertion. After NST, the clip was visible by ultrasound in 36 procedures (95%). In the other two patients, the ROLL-node injection was performed in a sonographically suspicious unclipped node (1), and near the clip under computed tomography guidance (1). Initial surgery successfully identified the ROLL-marked node with clip in 33 procedures (87%). Removed specimens in the other five procedures contained only the sonographically suspicious tumor-positive unclipped node (1), a node with signs of complete response but no clip (2), a clip without node (1), and tissue without node nor clip, and a second successful ROLL-node procedure was performed (1). Overall, 10 ROLL-marked nodes had no residual disease.

Conclusions
This study demonstrates that the ROLL procedure to identify clip-marked lymph nodes is feasible. This facilitates selective removal at surgery and may tailor axillary treatment in patients treated with NST.
Customized one-step preparation of sgRNA transcription templates via overlapping PCR Using short primers and its application in vitro and in vivo gene editing
Authors: Hu Zheng , Wang Li , Shi Zhaoying , Jiang Jing , Li Xiangning , Chen Yonglong , Li Kai , Luo Dixian 
Published: October 2019

Abstract
Overlap extension polymerase chain reaction (PCR) is a powerful technology for DNA assembly. Based on this technology, we synthesized DNA templates, which were transcribed into sgRNA in vitro, and further detected their efficiency of purified sgRNAs with Cas9 nuclease. The sgRNAs synthesized by this approach can effectively cleave the DNA fragments of interest in vitro and in vivo. Compared with the conventional method for generating sgRNA, it does not require construction of recombinant plasmids and design of primers to amplify sgRNA core fragment. Only several short primers with overlapped sequences are needed to assemble a DNA fragment as the template of sgRNA. This modified and simplified method is highly applicable and less time-consuming.
Short-acting β2-agonists (SABA) bronchodilator sales and outdoor mould in central France
Authors: Caillaud D. M. , Martin S. , Ségala C. , Dupuy N. , Thibaudon M. , Muti D. 
Published: October 2019

Background
Temporal ecological studies have shown that outdoor moulds are associated with severe asthma exacerbations, as emergency department visits or hospitalisations. The aim of this was to assess the associations between daily sales of short-acting β2-agonists (SABA), a specific and frequent treatment for control of mild asthma exacerbations in children and young adults, and outdoor mould concentrations in the central France area over a 5 year period.

Methods
The relationship between daily changes in mould (25 species) concentrations and daily SABA sales within a population of patients aged 6 to 39 years in a middle-size town of central France (approximately 127,000) was obtained from social security database and analysed with generalized additive models, taking into account confounding factors (air pollution, weather conditions, pollen counts and trend).

Results
Daily SABA sales (mean, SD) rose from 17.3 (9.7) in 2010 to 22.7 (12) in 2015. The relative risk (RR [95% CI]) of SABA sales associated with an interquartile increase in mould concentration was significant in the whole population for Alternaria 1.06 [1.002–1.12]. When the influence of age and sex was accounted for, the relationship was significant only in 6–12 years old males for Alternaria 1.21 [1.04–1.41] and Aspergillus–Penicillium 1.08 [1.04–1.12].

Conclusions
Daily SABA sales are positively associated with Alternaria spores in the general population of children and young adults. The association between daily SABA sales and temporal changes to Alternaria and Aspergillus–Penicillium in male children indicate that outdoor moulds contribute to asthma morbidity.
Effects of physical exercise on outcomes of cardiac (dys)function in women with breast cancer undergoing anthracycline or trastuzumab treatment: study protocol for a systematic review
Authors: Antunes Pedro , Esteves Dulce , Nunes Célia , Amarelo Anabela , Fonseca-Moutinho José , Afreixo Vera , Costa Henrique , Alves Alberto , Joaquim Ana 
Published: October 2019

ABSTRACT

Background
Cardiotoxicity is a known complication and one of the most adverse effects from the use of conventional treatments such as anthracyclines and trastuzumab in breast cancer (BC) care. This phenomenon has been associated with the restriction of therapeutic options and the increase of cardiovascular complications, which may compromise the survival of patients. Implementation of preventive strategies is an important approach for the management of this issue. Physical exercise has been proposed as a non-pharmacological strategy to counteracting cardiotoxicity. The aim of this protocol is to describe the rationale and methods for a systematic review of published randomized controlled trials (RCTs) that have analysed the effects of physical exercise on outcomes of cardiac (dys)function in women with BC undergoing neoadjuvant or adjuvant treatment containing anthracyclines and/or trastuzumab.

Methods and analysis
This is a protocol for a systematic review reported according to the PRISMA-P 2015 checklist. Randomized controlled trials (RCTs) will be included. The literature will be screened on MEDLINE, EMBASE, the Cochrane Central Register of Controlled Trials, ISI Web of Science and Scopus. The risk of bias of the included RCTs will be assessed using the Cochrane Collaboration’s tool. The primary outcomes will be systolic function (left ventricular ejection fraction), diastolic function (E/A’ ratio, deceleration time of early left ventricular filling, isovolumetric relaxation time, E/E’ septal and lateral ratio) and myocardial deformation imaging outcomes (strain and strain rate [measured in longitudinal, radial, or circumferential directions]). Secondary outcomes will be cardiac biomarkers (troponin I or T, high-sensitivity troponin I or T, brain natriuretic peptide, amino terminal of B-type natriuretic peptide). Data will be descriptively reported, and quantitative synthesis will also be considered if the included studies are sufficiently homogenous.

Discussion
This systematic review will help to understand the effectiveness of physical exercise on counteracting cardiotoxicity related to anticancer therapies in women with BC.

Systematic review registration
PROSPERO CRD42018096060.
Variable retention harvesting: conceptual analysis according to different environmental ethics and forest valuation
Authors: Galetto Leonardo , Torres Carolina , Martínez Pastur Guillermo J. 
Published: October 2019

AbstractBackgroundConceptual clarity is important to attain precise communication of scientific knowledge and to implement appropriate technological and policy actions. Many concepts referring to forest management are widely used by decision-makers, regardless of their complexity. Although the scientific and methodological issues of forestry practices are frequently discussed in the literature, their normative dimensions are rarely treated. Thus, linguistic uncertainty increases when different environmentally ethical perspectives and ways of valuing forests are considered. The objective was to compare different conceptualizations on the silvicultural systems suggested for forest management and the implications they have for conservation. We have conceptually contrasted high-intensity forestry practices with variable retention harvesting, considering different environmentally ethical perspectives and forest valuation alternatives.ResultsClear boundaries between clear-cutting, selective logging, and variable retention harvesting can be evidenced when different ethical points of view and alternatives in the human-nature relationships are considered. We have found a variety of definitions of variable retention harvesting that can be analyzed under different ethical positions. Sharply contrasting views on variable retention harvesting can be evidenced if nature is considered to be purely at human’s service or if it is conceptualized as humans co-inhabiting with nature. The latter position implies that the maintenance of ecological, evolutionary, and historical processes supported by unmanaged forest stands is a crucial step for forest management proposals based on variable retention harvesting.ConclusionsForestry practices that are focused on forest yields and that misinterpret functional uncertainty of forest functioning would be risky. Moreover, forestry with variable retention harvesting could imply good yields with reasonable conservation management in some contexts, while it could be unacceptable in other socio-ecological contexts. The improvement of conceptual clarity on the different meanings of variable retention harvesting and the development of indicators for forest management based on the variations of this concept can reduce controversies.
What is the nature and extent of evidence on methodologies for monitoring and evaluating marine spatial management measures in UK and similar coastal waters? A systematic map protocol
Authors: O’Leary Bethan C. , Stewart Bryce D. , McKinley Emma , Addison Prue F. E. , Williams Chris , Carpenter Griffin , Righton David , Yates Katherine L. 
Published: October 2019

Abstract
Background
Anthropogenic degradation of marine ecosystems is widely accepted as a major social-ecological problem. The growing urgency to better manage marine ecosystems has led to the increasing application of ‘spatial management measures’ including marine protected areas, sectoral (e.g. fishery) closures, and marine spatial planning. However, the designation of varied spatial management regimes is just the first step; achievement of objectives relies upon effective implementation, monitoring, evaluation and adaptation. Despite spatial management being a core component of the marine management portfolio, to our knowledge, there is no systematic overview of the evidence on methodologies available, and employed, to monitor and evaluate their effectiveness across social, economic and ecological outcomes.

Methods
This systematic map will examine existing evidence describing methodologies for monitoring the effects, and evaluating the effectiveness, of marine spatial management across ecological, social and economic outcomes. Our aim is to provide a resource for decision-makers, primarily in the UK but also internationally, that supports effective marine management, and to describe the current evidence base. Identification and evaluation of relevant studies will therefore be restricted to coastal countries identified by our Stakeholder Group as being relevant to the UK, and searches will be restricted to the period 2009 to 2019 to align with the current UK policy context. Searches for relevant grey and academic literature, published in English, will be conducted in four bibliographic search engines, Google Scholar, 38 organisational websites and one specialist data repository. Eligibility screening will be conducted first at title and abstract level, and then at full text. Coding and meta-data extraction from eligible studies will include: bibliographic information, general information about the spatial management measure studied, and methodological information on the monitoring and evaluation undertaken. Consistency checking amongst reviewers will be undertaken during screening, coding and data extraction phases. The outcome of the systematic map will be a database that displays the meta-data of identified relevant studies. Findings will be presented in a descriptive report detailing the evaluation approaches and analytical methodologies employed, and data collection methods applied and/or data required by relevant studies to inform evaluations on the effectiveness of marine spatial management measures.
Effectiveness of an osteopathic treatment on the autonomic nervous system: a systematic review of the literature
Authors: Rechberger Verena , Biberschick Michael , Porthun Jan 
Published: October 2019

Abstract

Objective
The objective of this systematic review was to evaluate the effectiveness of an osteopathic treatment on the autonomic nervous system (ANS). For this purpose, published primary studies were analysed and critically evaluated.

Method
To generate this review, 15 electronic databases were systematically searched for studies. Randomized clinical controlled trials (RCT) and clinical controlled trials (CCT) are included in the review and evaluated with appropriate assessment tools (Downs and Black Checklist and the checklist from Kienle and Kiene).

Results
23 published studies (10 RCT, 1 clinic multi-centre study, 1 CCT, 5 randomized cross-over studies, 5 randomized pilot studies and 1 single case study) are included in this review. The studies were evaluated with the assessment tools according to their quality. 3 studies are graded as high quality, 11 as moderate and 8 as low-quality studies.

Conclusion
The included published studies represent a good level of evidence. Due to a small number of subjects and no follow-ups, the methodological quality is rated as moderate. A significant change on the ANS was shown in studies including High-Velocity Low-Amplitude Techniques (HVLAT). No statement could be drawn in studies in which they used cranial osteopathic techniques due to the lack of methodological quality. A significant change on the ANS is shown in the treatment of the suboccipital region. In studies which evaluated the effectiveness of mobilization in the cervical and thoracic region, no statement could be displayed due to a low level of evidence. None of the findings in these studies have given statements if ANS activation takes place in the sympathetic or parasympathetic system.
Developing elderly men’s footwear sizing system based on their foot shapes
Authors: Kim Namsoon , Do Wolhee 
Published: October 2019

AbstractThe purpose of this study was to develop a shoe sizing system for elderly men in Korea based on previous studies which analyzed aspects and shapes of the sole of those who aged 60 years or more. The sizing system creation process included natural log-transformation, principle component analysis, multivariate linear regression, size range determination, and measurements calculation. Measurement interval was set up based on Korea standard (KS). Cross analysis was conducted for basic items to select an interval which indicated frequency of more than 5% by type. A measurement system was then established by type. As results of this study, a shoe sizing system was developed with an interval of 5 mm for foot length, an interval of 3 mm for the circumference of the top of the foot, and an interval of 6 mm for the circumference of the top of the foot within the same foot length standard. To reflect characteristics by type, respective shoe sizing systems in accordance with type were developed. 12 types of shoe sizing systems were suggested for elderly men. Our results indicate that shoe sizing system for elderly men by foot type could improve the suitability of shoes. They could give wider range of shoe sizes and high satisfaction. They can help shoe makers produce shoes with various purposes and functions for seniors.