bluuebunny/crossref_metadata_2025_split huggingface dataset:
Short-term Outcomes of Robotic- versus Laparoscopic-Assisted Total Gastrectomy for Advanced Gastric Cancer: A Propensity Score Matching Study
Authors: Yang Changdong , Shi Yan , Xie Shaohui , Chen Jun , Zhao Yongliang , Qian Feng , Hao Yingxue , Tang Bo , Yu Pei-Wu 
Published: January 2020

Abstract
Background: Few studies have been designed to evaluate the short-term outcomes for advanced gastric cancer (AGC) between robotic-assisted total gastrectomy (RATG) and laparoscopy-assisted total gastrectomy (LATG) alone. The purpose of this study was to assess short-term outcomes of RATG compared with LATG for AGC.
Methods: We retrospectively evaluated 116 and 244 patients who underwent RATG or LATG respectively. Besides, we performed a propensity score matching (PSM) analysis between RATG and LATG for clinicopathological characteristics to reduce bias and compared short-term surgical outcomes.
Results: After PSM, the RATG group had longer operation time (291.09±58.41 vs. 271.99±48.41min, p=0.007), less intraoperative bleeding (151.98±92.83 vs. 172.59±97.01ml, p=0.032) and more N2 tier retrieved lymph nodes (RLNs) (9.33±5.46 vs. 7.50±3.86, p=0.018) than the LATG group. Besides, the total RLNs of RATG was more but not statistically significant compared to LATG (35.09±12.93 vs.32.34±12.05, p=0.062). However, no significant differences were found between the two groups in terms of length of incision, proximal resection margin, distal resection margin, postoperative hospital stay. The conversion rate was 4.92% and 8.61% in the RATG and LATG groups, respectively, with no significant difference (p=0.198). The ratio of splenectomy was 1.7% and 0.4% respectively (p=0.503). There was no significant difference in overall complication rate between RATG and LATG groups after PSM (24.1% vs. 33.6%; p=0.102)and the grade II complications accounted for most of all complications in the two cohorts. The mortality was 0.9% and 0% respectively (p=0.322).
Conclusion : This study demonstrates that RATG is comparable to LATG in terms of short-term surgical outcomes.
Evaluation of radioactive 125I seed implantation for the treatment of refractory malignant tumors based on CT-guided 3D template assisted technique: efficacy and safety
Authors: Zhao Guang Sheng , Liu Song , Yang Liang , Li Chuang , Wang Ruo Yu , Zhou Jun , Zhang Yue Wei 
Published: January 2020

Abstract
Background: To observe the medium-and long-term clinical efficacy and safety of radioactive 125I seed implantation for refractory malignant tumors based on a CT-guided 3D template-assisted technique.
Methods: Twenty-five patients with refractory malignant tumors who received radioactive 125I seed implantation based on a CT-guided 3D template-assisted technique were selected. The post-operative adverse reactions were recorded; the number of puncture needles and particles used in the operation, dosimetric parameters, post-operative physical strength scores, and tumor response were statistically analyzed. The overall survival time and survival rate were counted, and the effect and prognosis were assessed.
Results: 125I seed implantation was successful in all patients and no serious complications occurred. The average number of implanted puncture needles was 17 (19.12±13.00) and the median number of particles was 52 (55.12±32.97). The D90 in the post-operative clinical target volume (CTV) (93.24±15.70 Gy) was slightly lower than pre-operative CTV (93.92±17.60 Gy; P>0.05). The D90 in the postoperative planning target volume (PTV) (142.16±22.25 Gy) was lower than the pre-operative PTV (145.32±23.48 Gy; P>0.05). The tumor responses at 6 months post-operatively: complete remission (CR), 20%(5/25); partial remission (PR), 48%(12/25); stable disease (SD), 24%(6/25); progressive disease (PD), 8%(2/25); CR+PR, 68%(17/25); and local control rate, 92%(23/25). The 6-, 12-, and 24-month survival rates were 100%, 88%, and 52%, respectively. The post-operative physical strength score (Karnofsky performance score, KPS) exhibited a gradual trend towards recovery, which rose to the highest value 12 months after seed implantation, then decreased slightly, but the average score was still > 90 points. There was one intra-operative pneumothorax and two patients with superficial malignant tumors developed skin ulcerations. Multivariate analysis of prognosis showed that tumor sites and types were independent risk factors affecting survival. The number of needles and particles, and template type were not factors that affected prognosis.
Conclusions: 3D template combined with CT-guided radioactive 125I seed implantation was shown to improve the rational distribution of radiation dose in the tumor target area because accurate radioactive 125I particle implantation was achieved. It has fewer complications and can further extend the overall survival and quality of life in patients with refractory malignant tumors.
Identification and Analysis of the FAD Gene Family in Walnuts (Juglans regia L.) Based on Transcriptome Data
Authors: Liu Kai , Zhao Shugang , Wang Shuang , Wang Hongxia , Zhang Zhihua 
Published: January 2020

Abstract
Background: Walnut fatty acids, the main component of walnut kernels, contain a large amount of unsaturated fatty acids, such as linoleic acid and linolenic acid, which are essential fatty acids in humans and have important effects on human growth and health. Fatty acid desaturase (FAD) is widely distributed throughout the biological world. Its main function is to remove hydrogen from carbon chains in the biosynthesis of unsaturated fatty acids to synthesize C=C double bonds.

Results: In the current research, 24 members of the JrFAD gene family were identified by bioinformatics analysis; the expression of fatty acid synthesis genes in walnut kernels at different developmental stages was analysed by transcriptome sequencing, and the expression of JrFAD3-1 , an enzyme gene for linolenic acid synthesis, was particularly prominent. The results showed that the relative expression level of FAD3-1 changed dramatically with the kernel development stage, and the expression changes showed a "bell shape". There was a significant positive correlation between the expression of JrFAD3-1 from 90-100 DAF (days after flower) and the content of alpha-linolenic acid from 100-130 DAF, with a correlation coefficient of 0.991. JrFAD3-1 can be considered closely related to Betula pendula and Corylus heterophylla .

Conclusion: 24 members of the JrFAD gene family consist of four subfamilies: the SAD desaturase subfamily, Δ7/Δ9 desaturase subfamily, Δ12/ω-3 desaturase subfamily and "front-end" desaturase subfamily. A key gene Jr FAD3-1 was obtained based on transcriptome data and there was a significant positive correlation between the expression of JrFAD3-1 from 90-100 DAF and the content of alpha-linolenic acid from 100-130 DAF. JrFAD3-1 can be considered closely related to Betula pendula and Corylus heterophylla .
Does photobiomodulation therapy combined to static magnetic field (PBMT-sMF) promote ergogenic effects even when the non-exercised leg is irradiated? A randomized, triple-blind, placebo-controlled trial.
Authors: Machado Caroline dos Santos Monteiro , Casalechi Heliodora Leão , Vanin Adriane Aver , de Azevedo Jônatas Bezerra , de Carvalho Paulo de Tarso Camillo , Leal-Junior Ernesto Cesar Pinto 
Published: January 2020

Abstract
Background The direct application of photobiomodulation therapy (PBMT) combined with a static magnetic field (sMF) (PBMT-sMF) to target tissues is shown to improve muscle performance and recovery. Studies have reported possible PBMT induced systemic effects. Notably, the extent of these effects on musculoskeletal performance and the optimal site of application remain unclear, although this information is clinically important because these factors directly affect the magnitude of the effect. We investigated the effects of PBMT-sMF on musculoskeletal performance and post-exercise recovery of muscles in exercised and non-exercised legs before the implementation of an exercise protocol.Methods This randomized, triple-blind placebo-controlled study included 30 healthy men randomly assigned to the placebo, exercised-leg, and non-exercised leg groups. Active or placebo PBMT-sMF was applied to 6 sites of the quadriceps muscle of both legs. An eccentric exercise protocol was used to induce fatigue. The following parameters were analyzed to evaluate exercise performance: peak torque assessed by maximal voluntary contraction (MVC), delayed muscle pain assessed by the visual analogue scale (VAS), muscle injury assessed by serum creatine kinase (CK), and fatigue assessed by serum lactate levels. Evaluations were performed before implementation of the eccentric (baseline) exercise protocol, as well as immediately after and 1, 24, 48, and 72 hours upon protocol completion. The Bonferroni post-hoc ANOVA test was used considering the level of statistical significance p <0.05.Results Compared to the placebo and systemic groups, irradiation with PBMT-SMF led to statistically significant improvement (p <0.05) with regard to all variables in the exercised-leg group. Results of irradiation in the non-exercised leg group were similar to those in the placebo group with regard to all variables.Conclusion Our results support the current evidence that irradiation of all exercised muscles produces ergogenic effects. PBMT-sMF improved performance and reduced muscle fatigue only when applied locally to muscles involved in physical activity.
The effects of exergaming on pain, postural control, technology acceptance and flow experience, in older people with chronic musculoskeletal pain: a randomised controlled trial
Authors: Ditchburn Jae-Llane , Schaik Paul van , Dixon John , MacSween Alasdair , Martin Denis 
Published: January 2020

Abstract
Background : Previous studies of exergaming for older people have reported mixed findings, but, overall, there is a broad indication of potential benefit. The purpose of this study was to assess the effects of exergaming, comparing the Interactive Rehabilitation and Exercise System (IREX®) with traditional gym-based exercise with no virtual stimuli (TGB), on pain, postural control, technology acceptance and flow experience in older people with chronic musculoskeletal pain. Methods : 54 older adults (age: 71 ± 5 years) with chronic musculoskeletal pain were randomised (stratified, blind card) to one of two groups: (a) completed exergaming using IREX® and (b) completed TGB exercise. Both groups completed two, 40-minute, exercise sessions (matched for intensity, duration and movement patterns) a week for six weeks. The sensory, emotional and motivational dimension of pain was measured using the Multidimensional Affect and Pain Survey (MAPS) questionnaire and intensity, with a numeric rating scale. Postural Control was measured as sway using a Kistler™ force platform. Technology acceptance was measured with the Unified Theory of Acceptance and Use of Technology (UTAUT) and flow experience with the Flow State Scale (FSS) questionnaires. Rating of Perceived Exertion (BORG RPE) and HR were also recorded during all sessions. Results : There were no significant differences in objective or subjective measures of physical demand and hence the exercise groups were matched. There were significant improvements in pain and balance in the exergaming group compared to the TGB group. Although significant intervention effects on technology acceptance were found in social influence and behavioural intention in the TBG group, both groups demonstrated significant increases in all of the technology acceptance variables over time. In terms of flow experience, concentration at task was significantly influenced in the TGB group and significant increases in flow experience variables were observed in both groups (TGB and exergaming). Conclusion : Exergaming has potential to alleviate pain and improve balance in older people with chronic musculoskeletal pain. Both forms of exercise are acceptable, intrinsically motivating and show evidence of benefit to older people with chronic musculoskeletal pain.
Genetic polymorphism of the CYP2C19 gene in the Foshan area of Guangdong Province
Authors: yuan Xiao wen , Yuan Shi-yun , Qiu Quan , Luo Zi-yuan , Zhao Xue-feng 
Published: January 2020

Abstract
Background: The CYP2C19 gene is highly polymorphic, and CYP2C19 is involved in the broad interindividual variability of the clinical efficacy of certain clinical medications, such as clopidogrel. However, data on the CYP2C19 genotype in the Chinese population of the Foshan area of Guangdong Province are scarce. The purpose of this study was to determine CYP2C19 genetic polymorphisms in patients in the Foshan area and to compare the CYP2C19 genotype frequencies in different populations to determine the allele distribution pattern to identify the most appropriate prescription. Methods: The CYP2C19 gene was detected in 1231 patients on a gene chip platform, and the genotype frequencies of CYP2C19 in Foshan populations from different populations were compared. Results: The frequencies of CYP2C19*1, *2 and *3 in the Foshan population were 63.89%, 30.46% and 5.65%, respectively. For the three metabolic types, the frequency associated with the rapid metabolism type (*1/*1) was 41.51 [95% confidence interval (CI) 40.11 to 42.91%]; that for the intermediate metabolism type (*1/*2, *1/*3) was 44.76% (95% CI 43.34 to 46.18and that for the slow metabolism type (*2/*2, *2/*3, *3/*3) was 13.73% (95% CI 12.75 to 14.71%). In the Foshan population, the frequencies of the CYP2C19 *2 and *3 alleles were similar to those previously reported for Chinese and other Asian populations. Conclusion: Our study the genetic basis of CYP2C19 polymorphism in the Foshan population. Our results will potentially contribute to the improvement of pharmacotherapy effectiveness by providing personalized medicine for the Foshan population. Key words: Polymorphism; Genetic; Pharmacogenetics
Emerging high-risk ST101 and ST307 carbapenem-resistant Klebsiella pneumoniae clones from bloodstream infections in Southern Italy
Authors: Loconsole Daniela , Accogli Marisa , Lisa De Robertis Anna , Loredana Capozzi , Angelica Bianco , Anna Morea , Rosanna Mallamaci , Michele Quarto , Antonio Parisi , Chironna Maria 
Published: January 2020

Abstract
BackgroundCarbapenem-resistant Klebsiella pneumoniae (CR-KP) is an urgent public health issue in Italy. The pattern of resistance is due mainly to dissemination of carbapenemase genes. Molecular characterization of carbapenem-resistant Klebsiella pneumoniae (CR-KP) strains was performed over a three-year period. In-depth analysis was performed on a subset of emerging CR-KP ST101 and ST307 clones.MethodsA prospective study was performed on 691 patients with CR-KP bloodstream infections hospitalized in 19 hospitals located in three large provinces in Southern Italy. Carbapenemase genes were identified via genotyping methods. Multi-locus sequence typing (MLST) and Whole Genome Sequencing (WGS) were carried out on ST101 and ST307 isolates.ResultsAmong the CR-KP isolates, blaKPC was found in 95.6%, blaVIM was found in 3.5%, blaNDM was found in 0.1% and blaOXA-48 was found in 0.1%. The blaKPC-3 variant was identified in all 104 characterized KPC-KP isolates. MLST of 231 representative isolates revealed ST512 in 45.5%, ST101 in 20.3% and ST307 in 18.2% of the isolates. cgMLST of ST307 and ST101 isolates revealed presence of more than one beta-lactam resistance gene. Amino acid substitution in the chromosomal colistin-resistance gene pmrB was found in two ST101 isolates.ConclusionsST512 is widespread in Southern Italy, but ST101 and ST307 are emerging since they were found in a significant proportion of cases. Aggressive infection control measures and a continuous monitoring of these high-risk clones are necessary to avoid rapid spread of CR-KP, especially in hospital settings.
Research priorities for rehabilitation and aging with HIV: a framework from the Canada-International HIV and Rehabilitation Research Collaborative (CIHRRC)
Authors: O'Brien Kelly K , Ibáñez-Carrasco Francisco , Solomon Patricia , Harding Richard , Brown Darren , Ahluwalia Puja , Carusone Soo Chan , Baxter Larry , Emlet Charles , Restall Gayle , Casey Alan , Ahluwalia Amrita , Quigley Adria , Terpstra Alex , Iku Nkem 
Published: January 2020

AbstractBackgroundPeople living with HIV are living longer, and can experience physical, mental and social health challenges associated with aging and multimorbidity. Rehabilitation is well positioned to address disability and maximize healthy aging. An international collaborative network, called the Canada-International HIV and Rehabilitation Research Collaborative (CIHRRC), works to guide this emerging field.In this article, we report findings from CIHRRC’s aim to identify emerging research priorities in HIV, aging and rehabilitation from the perspectives of people living with HIV, clinicians, researchers, representatives from community organizations and policy stakeholders.MethodsWe conducted a multi-stakeholder mixed-method international consultation with people living with HIV, researchers, clinicians and representatives of community-based organizations to identify research priorities in HIV, aging and rehabilitation. Stakeholders identified research priorities during a one-day International Forumcomprised of presentations and facilitated discussion. We collated and analyzed data using content analytical techniques, resulting in a framework of research priorities.ResultsSixty-nine stakeholders from countries including Canada (n=62;90%), the United Kingdom (n=5;7%), United States (n=1;1%) and Australia (n=1;1%) attended the International Forum on HIV, Aging and Rehabilitation. Stakeholders represented community-based organizations (n=20;29%), academic institutions (n=18;26%), community or institutional healthcare organizations (n=11;16%), research or knowledge production organizations (n=10;14%), and organizations representing government or industry (n=10;14%). TheFramework of Research Priorities in HIV, Aging and Rehabilitationincludes seven research priorities: 1) nature, extent and impact of disability, concurrent health conditions and chronic inflammation with HIV; 2) prevalence, severity and impact of frailty; 3) community and social participation aging with HIV; 4) strategies for chronic disease management and healthy aging with HIV; 5) facilitators and barriers to access to and engagement in, rehabilitation; 6) effectiveness of rehabilitation interventions for healthy aging with HIV; and 7) advancing development and use of patient reported outcome measures in HIV and aging. The Framework highlights methodological considerations to approach the priorities and the importance of knowledge translation and exchange to apply research knowledge into practice, programs and policy.ConclusionsThese priorities offer a foundation for collaboration among international and multidisciplinary teams to advance the field of HIV, aging and rehabilitation in order to promote healthy aging with HIV.
Etoricoxib decreases mouse subchondral bone mass and biomechanical properties in early osteoarthritis
Authors: Liu Bo , Ji Chen-Chen , Shao Yi-Jie , Liang Ting , He Jia-Heng , Jiang Hua-Ye , Chen Guang-Dong , Luo Zong-Ping 
Published: January 2020

Abstract
BackgroundEtoricoxib, a selective Cyclooxygenase-2 (COX-2) inhibitor, is commonly used in osteoarthritis (OA) for pain relief. The purpose of our study was to investigate the effects of Etoricoxib on mouse subchondral bone in early OA.MethodsOA was induced via destabilization of the medial meniscus (DMM) in C57BL/6J mice. After surgery, the mice were randomly and equally divided into five groups: a sham-operated control group (Sham group), an osteoarthritis (OA) group (DMM group), an OA treated with Etoricoxib 5mg/kg (DMM+E5) group, an OA treated with Etoricoxib 10mg/kg (DMM+E10) group, and an OA treated with Etoricoxib 20mg/kg (DMM+E20) group. Mice in the Sham group and DMM group were injected with a similar dose of vehicle (40% ethyl alcohol–saline solution). Four weeks after treatment, mice were euthanized. Micro computed tomography (Mirco-CT) analysis, Safranin O-Fast Green staining, hematoxylin and eosin (HE) staining were performed to evaluate morphological and structural changes. In addition, atomic force microscopy (AFM) analysis was performed to evaluate changes in the elastic modulus. Furthermore, changes in microstructure were detected by scanning electron microscopy (SEM).ResultsEtoricoxib inhibited osteophyte formation in the subchondral bone. However, it also reduced the bone volume fraction (BV/TV), lowered trabecular thickness (Tb.Th), and more microfractures and pores were observed in the subchondral bone. Moreover, Etoricoxib reduced the elastic modulus of subchondral bone. Furthermore, exposure to Etoricoxib further increased the empty/total osteocyte ratio of the subchondral bone. In cartilage and synovium, Etoricoxib did not significantly change the Osteoarthritis Research Society International (OARSI) score, the modified Mankin score, and the synovialitis-score versus the DMM group. Conclusion﻿Our results demonstrate that although Etoricoxib can relieve the pain induced by OA, it also has adverse effects on subchondral bone in early OA.
A Comparison of Global Surgery Tariffs and the Actual Cost of Bills at Hazrate Rasoole Akram Educational and Medical Center
Authors: Abutorabi Ali , Radinmanesh Maryam , Rezapour Aziz , Afshari Mahnaz , Taheri Ghasem 
Published: January 2020

Abstract
Backgroundthe health service tariff is an appropriate policymaking tool and the financial leverage of the health system control which affects quality, availability, cost, efficiency, equity and accountability of health services. Global surgeries include 91 common cases of general and specialized surgeries in hospitals; fixed tariffs are annually defined for these surgeries, and insurance companies must pay medical centers based on these tariffs. The aim of this study was to examine and compare hospital bills with global surgery tariffs at Hazrate Rasoole Akram Educational and Medical Center in 2017.MethodsThis descriptive-analytic study was conducted retrospectively and compared the global and actual costs of global surgeries performed in the third quarter of the year 2017 at Hazrate Rasoole Akram Educational and Medical Center. Required data on the actual costs of surgeries was collected through the Hospital Information System (HIS) and patients’ records. Information on the global costs was obtained from the Annual Circulars of Insurance Council for the studied period about the cost of global surgeries. Linear regression (STATA13 software) was used to investigate the effect of items on tariff and invoice differences; concerning other calculations, EXCEL software was used.ResultsThe highest frequency of global surgeries was related to ophthalmic surgery which accounted for approximately half of total surgeries performed at Hazrate Rasoole Akram Hospital. The most significant difference between global tariff and invoice was also related to ophthalmic surgery (6502876538 Rials a year).Overall, the actual hospital bills were much higher than the tariffs approved for global surgeries, and the total difference was 15913703073 Rials. The results revealed that there was a significant relationship between some of the items such as the cost of operating rooms, anesthesia and other services.ConclusionsReferral hospitals which are at the level three of referral networks usually treat more complex patients; this should be taken into account when defining surgery tariffs of these centers. On the other hand, hospitals need to control the costs and reduce the end cost of these surgeries by improving clinical management and cost management. In addition, prospective and case-based payment methods can control health costs.