bluuebunny/crossref_metadata_2025_split huggingface dataset:
Histological analysis of morphostructural changes in bronchial and lung tissues in cattle fetuses during chlamydia infection in Perm Krai and Tyumen Oblast of the Russian Federation
Authors: Tatarnikova N A , Kostarev S N , Sereda T G 
Published: September 2020

Abstract
Between 2017 and 2019, 543 cattle (cows` aborted fetuses, stillborn calves) were studied in Perm Krai and Tyumen Oblast of the Russian Federation for the existence of chlamydia infection. Blood serum samples and fingerprints were analyzed from the animals, and a positive result was registered in 48 animals, which is about 9% of the population. Chlamydia was diagnosed on the basis of clinical signs of disease, passive hemagglutination test and microbiological studies. From 23 aborted and stillborn fetuses, biological material was taken for electron microscopic examination. In order to study pulmonary tissue injuries under the influence of chlamydia, a histological analysis of bronchi and lung aborted and stillborn calves was carried out. All three components of the mother-placenta-fertility system were found to be affected by bovine chlamydia. A characteristic pathological-anatomical sign of chlamydia in fetal death is the formation of common swelling, both tissue and cavity localization. Histological examination of organs and tissues traces changes in the general pathological nature of the developing vascular level, alternative processes, immunopathological reactions, systemic inflammatory changes in histochemical barriers. Morphological changes in the pulmonary tissue of the fetuses of different gestation terms indicate damage to the aero-hematic barrier with the possibility of respiratory disorders in the uterine period.
Nutritional medium for differentiation of malignant anthrax
Authors: Pavlenko Igor V , Gryn Svetlana A , Markova Evgenia V , Matveeva Irina N. , Skotnikova Tatyana A , Eremets Vladimir I. , Smolentsev Sergey Yu 
Published: September 2020

Abstract
The purpose of the work is to develop a nutrient medium for differentiation of bacillus anthracis from soil aerobic bacilli. In order to achieve the set goal, we used the method of introduction into the environment of cultivation of microorganisms separated from animals and objects of external environment (water, soil, feed, air, scrapes from different surfaces suspected of contamination by their bacillus anthracis) of nutrient substrate - sucrose, used by bacteria of Bacillus genus for synthesis of levan - product of their metabolism, a sign absent in bacillus anthracis. This feature is essential for identification and differentiation of bacillus anthracis from closely related saprophytes. To identify and differentiate the bacillus anthracis, the microbes isolated from the external environment were cultivated in a nutrient medium consisting of meat-and-peptone agar (MPA) and stimulator (inductor) synthesis of levan sucrose in the amount of 10% to 100 ml of melted agar. The proposed nutrient media was prepared as follows. Meat-peptonic agar (500 ml) was melted at 100°C, 10 g of sucrose was added per 100 ml of medium and after the complete dissolution of sucrose, the nutrient medium was poured into Petri dishes and used for sowing the studied material for identification and differentiation of grown crops. The efficiency of the method has been tested in production experiments with positive evaluation. For this purpose, soil samples taken from the territory of old cattle cemeteries were fractionally sown on MPA and thermostatted for 16-18 hours at 37°C and examined crops for the presence of matte and rough (R-form) colonies.
Studying the metabolism of horses when feeding them zeolite-sapropel feed additives in the conditions of Yakutia
Authors: Grigorev M F , Sidorov A A , Grigoreva A I , Sysolyatina V V 
Published: September 2020

Abstract
The article presents the results of study on the effect of zeolite-sapropel feed additives on the metabolism of mares in Central Yakutia. Three groups of horses were formed to conduct scientific experiments. These groups were selected based on the principle of analogues, which took into account age, body weight and fatness. The control group got a basic ration. The experimental groups got a zeolite-sapropel feed additives to the basic ration in doses of 0.5 g of zeolite per kg of body weight with 200 and 300 g of sapropel. The study of the absorption of nutrients determined the effect of zeolite-sapropel feed additives on the intensity of nutrient uptake. Animals of the I and II experimental groups digest better: dry matter by 0.89% and 1.10%, organic matter by 0.90% and 1.17%, crude protein by 1.07% and 1.48%, crude fat by 2.16% and 5.08%, crude fiber by 1.70% and 2.43%, nitrogen-free extractives by 0.63% and 0.77%. Thus, the studies have shown that mares of the experimental groups fed with zeolite-sapropel additives, better use nitrogen, calcium and phosphorus. The studies have proved the effectiveness of using zeolite-sapropel feed additives when feeding mares in Yakutia.
Evaluation of the effect of fish yersiniosis on the course of antioxidant protection processes in trout
Authors: Droshnev A E 
Published: September 2020

Abstract
Indicators that characterize the state of lipid peroxidation and antioxidant protection in rainbow trout grown in the North-West of the country in normal conditions have been determined. It was found that the amount of malondialdehyde, antioxidant enzymes and total phospholipids had no significant changes in the samples taken in the farms of the Leningrad region and the Republic of Karelia, while the concentration level depended on the age of the fish. The maximum content of the final products of lipid peroxidation malondialdehyde was recorded in fish weighing 600-720 g, which is associated with a high fat content in the diet and the inability of the body to metabolize them completely. The developing pathological process in the body of fish when infected with Yersinia accelerated the intensity of lipid peroxidation, as a result, toxic lipid peroxides accumulated in organs and tissues, and the concentration of malondialdehyde increased from 65.4 to 95.2 %. There were also decreases in the activity of antioxidant protection: the value of catalase decreased from 6.7 to 23.7 %, peroxidase 7.1-19.5 %, the maximum drop in the activity of ceruloplasmin 9.5 %.
Adaptability of new varieties of seed peas in the Krasnoyarsk forest-steppe
Authors: Kozhukhova E V , Baykalova L P , Savinich E A 
Published: September 2020

Abstract
In the process of breeding work with the Pisum sativum culture, its manufacturability increases due to the introduction of new economically valuable features, as a result of the influence of abiotic factors on pea varieties is transformed. The work is relevant in timely identification of changes in influences and analysis of these transformations on the reconstructed genotype are important in breeding evolution. The material for the study was 12 samples of five varieties of Pisum sativum. The adaptability and stability indices of the samples – Indicator of the stability level of the variety, stability index, stress tolerance, breeding value, and the dependence of the adaptive properties of the culture on the complexity of its genotype were determined. The stress tolerance of the varieties was chaotic; its relationship with the evolution of the genotype is very weak. A strong positive relationship was revealed between the complication of the genotype and the coefficient of variation; a strong negative relationship was revealed between the resistance and stability index of the varieties. Strong negative dependencies were revealed between the evolution of culture and the stability index, as well as breeding value.
Expression of receptors in cow’s oviductal epithelial cells to prostaglandins E2, D2 and F2α
Authors: Kusheev Ch , Pavlov S , Cao J , Lomboeva S , Abidueva L 
Published: September 2020

Abstract
The significance of studying a condition of the reproductive system in productive animals, especially in dairy cattle, is directly proportional to the population’s needs in dairy, lactic acid, and other livestock products. The article presents the results of studies conducted at the Agricultural university of Hohhot city, China. The aim of the study was to identify the expression of receptors for commercial prostaglandins. The object of research was the biological material obtained from slaughter cattle, namely, the section of the cow’s oviducts. The subject of research was isolated epithelial cells. Expression was assessed by the level of intracellular Ca2+ and cAMP when exposed to PGE2, PGD2 and PGF2 of various concentrations from 10−5 to 10−9, also was using an agonist Butaprost. As a result, the concentration of PGE2 at 10−5 mol/L, PGF2α at 10−5 mol/L and 10−6 mol/L significantly contributed to an increase in the level of intracellular calcium (Ca2+) in the epithelial cells of the cow’s oviducts. The increase in the concentration of PGE2, PGD2, and Butaprost led to an increase in the level of cAMP concentration in epithelial cells of cow’s oviducts. EP1, EP2, EP4, FP, DP, TP receptors are available on cow’s oviductal epithelial cells. EP3 and IP receptors were not found on cow’s oviductal epithelial cells. Expression of the EP2, EP4, DP, FP receptor genes is well expressed, unlike EP1 and TP.
Anatomical structure of the Alhagi pseudalhagi stem
Authors: Ochirova K S , Dordzhieva V I , Voloshina T V , Geldieva Z A 
Published: September 2020

Abstract
The present research focuses on studying the anatomical structure of annual shoots of Alhagi pseudalhagi, growing in the Karakum Desert in Turkmenistan. The Camelthorn plays a huge role in the desert biogeocenosis stability, restrains the advance of sand dunes, and is the main component of the country’s natural pastures. The non-tufted type of structure of Alhagi pseudalhagi offshoots was established in the work; the degree of development of the photosynthetic bark parenchyma of the stem was identified. Diagnostic features of the offshoots were determined - the presence of a two-layer epidermis, unicellular receptacles, a continuous ring of conductive tissues, and a filled core of the stem. The nature of the distribution of reservoirs in the stem and the degree of development of mechanical and conductive elements in wood and bast are considered. The results of the study can be used in further studies of the Alhagi genus in the field of botany, physiology, ecology of desert biogeocenoses.
Technical efficiency of spring rapeseed protection scheme
Authors: Baikalova L P , Bobrovsky A V , Kryuchkov A A 
Published: September 2020

Abstract
The species composition of weeds and pests in the crops of spring rape of the variety Nadezhny 92 was determined, the technical effectiveness of the protection scheme of the Syngenta company was evaluated. The results of the study showed that the prevailing species were cereal weeds of 17.9 units / m2. Dicotyledonous weeds amounted to 13.6 units / m2. A decrease in the moisture supply of the vegetation period entailed an increase in weediness. The highest weediness was recorded in 2019, when the number of weeds was higher than in 2017 and in 2018 on 23.5 - 18.0 pcs / m2. Of the pests, cruciferous fleas, rapeseed beetle, rape sawfly, and caterpillars of cabbage were found in the crop. Spring rape protection scheme: presowing treatment with Cruiser Rapeseed, KS, Zellek Super, KE Galera Super, BP, Karate Zeon, MKS showed high technical efficiency. Technical efficiency against cereal weeds was 84.4%, against dicotyledons - 90.4%. On average, the protection effect on spring rape crops was 86.3%. The use of an insecticide allowed reducing the number of pests in crops below the economic threshold of harmfulness or completely destroying them.
Toxicological, antibacterial and anti-quorum activity of extracts of medicinal plants Betula spp., Hypericum spp. and Angelica spp.
Authors: Inchagova K S , Kosyan D B , Rusakova E A , Duskaev G K 
Published: September 2020

Abstract
Quorum sensing plays an important role in the infectious process development in plants, animals and humans. Inhibiting this phenomenon may be a new way to treat bacterial infections in therapeutic practice. Similar studies are being conducted all over the world and several approaches to inhibiting quorum-dependent processes are currently being presented. Folk remedies, which include, in particular, medicinal plants, can become an alternative tool for fighting bacterial pathogens to traditionally used antibacterial substances that have lost their former effectiveness, due to the widespread spread of antibiotic resistance. In this work, the toxicological, antibacterial and anti-quorum properties of the extracts of Betula spp. leaf, Hypericum spp. herb and Angelica spp. root were studied. Testing the extracts of Betula spp. leaf, Hypericum spp. herb and Angelica spp. root showed a minor toxicological effect on the recombinant luminescent strain Escherichia coli, which is manifested at high concentrations of the substance being tested. Similar results were obtained on another test object Stylonychia mytilus, for which the maximum toxicological effect was observed in the concentration range of 5.0-10.0 mg / ml for Betula spp. leaf and Hypericum spp. herb, and 10.0 mg / ml for Angelica spp. root. Against this background, quorum-inhibiting activity of the studied plant extracts was registered, increasing in the series Angelica spp. → Hypericum spp. → Betula spp.
Immunomorphological changes in bird staphylococcus
Authors: Pavlova A V , Pimenov N V , Konstantinov A V , Bordyugova S S 
Published: September 2020

Abstract
Staphylococcosis in birds is everywhere and belongs to the number of ubiquitous bacterioses. The etiology and pathogenesis of this infectious disease are directly related to the immune status of susceptible individuals. In a practical aspect, the evaluation of the immune system is based on the characterization of immunocompetent organs. A special place in solving the problem of treatment of staphylococcal infection is the study of age-related morphology of immunocompetent organs. The article presents the data of histological studies of the thymus, spleen, and bursa of Fabricius during critical periods of broiler chickens’ cultivation when exposed to them with immunocorrection drugs of plant origin. Morphological changes in the immunocompetent organs when using the antibiotic florfenicol and the echinacea purpurea root against the background of the antigenic effect of the chickens Staphylococcus aureus on the body indicate the immunotropic effect of the antibiotics of the amphenicol group, as well as the immunomodulating properties of the drug based on echinacea purpurea.
Structure peculiarities of Pinus sylvestris L. consortium
Authors: Turmukhametova N V , Bedova P V , Vorobeva I G 
Published: September 2020

Abstract
The structure of Pinus sylvestris L. consortium of various ontogenetic conditions in the regions of the Republic of Mari El and the city of Yoshkar-Ola in the territory of Russia is described. Based on modern methods of collecting arthropod animals in tree crowns and studying the structure of consortium, data analysis has been performed. With P. sylvestris coenopopulations, 73 species of Insecta and Arachnida are associated. The dominant species diversity is Coleoptera. The trophic groups of Insecta and Aranei, the groups in the breadth of the range of nutrition and harmfulness are characterized. It is shown that the diversity of Insecta and Aranei complexes in P. sylvestris coenopopulations is determined by the age features of the tree and the ecological conditions of the study areas. Consideration of the ontogenetic state of the determinant of the consortium and analysis of the formation of different types of integumentary tissue contributes to the prediction of the stages of colonization and damage to trees by harmful Insecta. It was suggested that for the detailed characterization of the consortium, it is necessary to organize a long-term monitoring of the state of tree stands and the composition of their consorts using the population-ontogenetic approach.
Identification of relationship of polymorphic variants of lactoferrin gene (LTF) in cows with milk production indicators depending on their lineage
Authors: Zinnatov Farit F , Zinnatova Farida F , Akhmetov Takhir M , Volkov Renat A , Hairullin Damir D , Bikchantaev Irek T , Valieva Elvira A , Yu Smolentsev Sergey 
Published: September 2020

Abstract
Dairy farming is striving to achieve positive results both in milk production increase and quality improvement. Unfortunately, the conventional breeding selection system does not consider the genotypic identity that reflects the level and direction of the processes occurring in the organism. The modern breeding selection is based on the selection by a set of features. In breeding, those animals that have more valuable characteristics are considered valuable. Animal husbandry can achieve great success only when analyzing the biology of the animal body and productivity. In order to increase the genetic potential of cows in Russia, it is necessary to take into account genetics and milk productivity. Analysis of milk productivity of cows with different LTF genotype in their pedigrees showed that the maximum milk yield was in cows of the Ayvengo line with the LTFAB genotype. These cows were distinguished from other cows by their high protein content, and these animals had the lactoferrin gene. And the animals of the Ayvengo line differed in the level of fat in milk. The comparative PCR-RFLP analysis of the blood DNA samples of the first-calf Holstein cows showed the benefit virtually by all milk production indicators (milk yield, milk protein and fat), including by somatic cell content in milk of the Ayvengo and Sovereign line cows with the LTFAB genotype. The Aydial line stock with the LTFAB genotype is worthy of notice regarding the selection activity.
Characterization of high virulent strains of equine rhinopneumonitis virus
Authors: Alexeyenkova S V , Yurov K P 
Published: September 2020

Abstract
This article presents results of screening samples collected from horses during acute outbreaks of abortion and neurological disorders recorded in 2019-2020 in Russia. The signs of disease were characterized by severe hemorrhagic vasculitis. Massive hemorrhages were observed in organs of aborted fetuses and dead horses. Equine herpesvirus type one was isolated in cell culture. Testing to identify genes encoding glycoprotein B and polymerase catalytic subunit of certain viral isolates were carried out. Virus isolated from horses with signs of encephalomyelitis from the Northwest region had a high degree of phylogenetic relationship with the strain “89c25” of equine herpesvirus type one from Japan, 1989. The same virus was found in horses with signs of rhinopneumonia from the Ural region. The virus isolated after equine abortion from the North Caucasus region had a high degree of phylogenetic relationship with the strain “IT19m10” of equine herpesvirus type one from Italy, 2019. It was established that all viral isolates belong to non-neuropathogenic genotype - A2254.
Evaluating the UV radiation effectiveness in industrial aquaculture
Authors: Bychkova L I , Nikiforov-Nikishin D L , Ponomarev A K , Bugaev O G 
Published: September 2020

Abstract
The paper presents an analysis of the bacterial purification effectiveness by UV radiation of wastewater in modern industrial aquaculture recirculation systems. the effectiveness of water purification in the ultrasound system by a set of filters for mechanical treatment and ultraviolet radiation is experimentally established. UV lamp allows to process water with rays that effectively destroy the organic matter formed in it. Pathogenic and conditionally pathogenic genera, such as: Aeromonas, Pseudomonas, Staphylococcus, Vibrio, Enterobacteria were differentiated.
Age-related changes in the anti-radical defense system in the tissues of Yesso scallop Mizuhopecten Yessoensis (JAY1857)
Authors: Zhukovskaya A F , Dovzhenko N V , Slobodskova V V , Chelomin V P 
Published: September 2020

Abstract
The glutathione level and the total oxyradical scavenging capacity in the digestive gland and the gills of the Yesso scallop Mizuhopecten yessoensis individuals of different ages (2-7 years) have been studied. The research revealed the tissue-specific high glutathione content in the gills of the scallop and the age-related decline tendency of glutathione level in the digestive gland. TOSC as a complex antioxidant capacity indicator is less tissue-specific but also depends on age.
Cytogenetic disturbances in the apical meristem cells of the urban plantations depending on the level of anthropogenic impact
Authors: Ladnova G G , Kurochitskaya M G , Chernikov N E 
Published: September 2020

Abstract
The scope of this research covered cytogenetic indexes of the apical meristem cells of the urban plantations of small-leaved linden (Tilia cordata L.) and silver poplar (Populus alba L.) growing in the areas of different anthropogenic pollution load, such as parks, public gardens, and urban plantation areas along the municipal motorways. The cytogenetic disturbances of the linden and poplar apical meristem cells were studied based on the incidence of micronuclei and nucleus protrusions of different forms. Buds for the analysis were collected from the tree height of 1.5-2 m in the spring season, in March-April. The collected buds were preserved in aceto-alcohol, then the apical meristem cell microslides were prepared and coloured with acetocarmine. The karyological characteristics were analysed and quoted according to the method of L. P. Sycheva. It was demonstrated that the cytogenetic disturbances with the greatest incidence of micronuclei and nucleus protrusion of various types are significantly more frequent in the apical meristem cells of the small-leaved linden and silver poplar buds collected from the plantations along the urban motorways compared to the same indexes of the trees growing in parks and public gardens. Compared to the same cells of silver poplar, the apical meristem cells of the small-leaved linden buds were found to be more sensitive to the technogenic pollution of the environment. The apical meristem cells of the buds of the studied trees and small-leaved linden in particular may serve as the genotoxicity indicators of the urban environment.
Productivity and grain quality of a new spring wheat variety Stolypinka depending on the rates of mineral fertilizers under conditions of the Pre-Baikal region
Authors: Sultanov F S , Yudin A A , Gabdrakhimov O B 
Published: September 2020

Abstract
The presented article reveals the extent of mineral fertilizers impact on plant growth and development, and the ripening periods of a new spring wheat (Triticum aestivum L.) cultivar Stolypinka. The research objectives included the study of the influence of mineral fertilizer rates on yield structure, productivity, grain quality and economic efficiency of the variety Stolypinka. Field observations and the laboratory analysis of the data obtained proved the positive effect of mineral fertilizers on the yield structure components (the amount of productive stems, the number of kernels in a spike, the weight of 1000 seeds) in the studied cultivar. As a result of the research it has been found that the application of fertilizers at the rate for the planned yield is optimal for obtaining the maximum yield 3.5 t/ha of good quality grain containing the crude protein 15.8 % and gluten 32.3 %. In terms of economic efficiency, at the rates of fertilizers application N30 and N60 we have got the highest payback output (8.2 and 9.0 thousand rub./ha, respectively) and the lowest prime cost of 1 t grain (4.6 and 4.7 thousand rub./ha, respectively). The highest level of profitability (92.3 %) was observed in the option with the application of sole nitrogen at the rate 30 kg of active substance per 1 ha. Of the variants with a complex mineral fertilizer, using N60P45К45 is the most expedient in case of the gross grain harvest in the amount recouping the invested production costs.