bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of Potential Genes in Her2+ Breast Cancer by Co-expression Network Analysis
Authors: Li Xinyang , Wang Yukun , Wang Ziming , Yao Ge , Fan Jiahao , Liang Gaofeng , Wang Xinshuai 
Published: May 2020

Abstract
Background

Breast cancer (BC) is one of the most common malignancies in women all over the world. For patients with human epidermal growth factor receptor 2 positive (Her2+) BC, although the widespread use of anti-Her2 therapy has make for survival increased, improved survival has led to an increased demand for better management and diminish toxic side effects of anticancer treatments. This study aimed to identify the potential biomarkers associated Her2 + BC in order to improve the overall management of BC treatment.
Results

Our research downloaded GSE54140 gene expression datasets from the Gene Expression Omnibus data sets, and used weighted gene co-expression network analysis (WGCNA) to developed a scale-free gene co-expression network to explore the associations between gene sets and clinical features. A total of 60 modules were analyzed, and found that the skyblue3 module was significantly related to Her2 + BC. The function of 93 genes in the skyblue3 module was annotated by DAVID bioinformatics tool, and it was demonstrated that the function of the module was mainly related to nuclear-transcribed mRNA catabolic process, cytosol, and oxidoreductase activity. Based on the WGCNA and Cytoscape software analysis, 9 hub genes (PGAP3, PPP1R1B, PNMT, ERBB2, CISD3, CRKRS, TCAP, STARD3, and NEUROD2) were identified. The Human Protein Atlas database detected that the protein level of PGAP3, PPP1R1B, PNMT, ERBB2, CISD3, CRKRS, TCAP, and STARD3 in tumor tissues were different from normal tissues. And survival analysis shows that PGAP3, PNMT, ERBB2, TCAP, and STARD3 were negatively associated with the overall survival (P < 0.05).
Conclusions

In total, 9 candidate biomarkers were identified by comprehensive analysis, among which, the co-expansion of PGAP3, CRKRS, STARD3 and NEUROD2 related to ERBB2 may be associated with the occurrence of Her2 + BC. In addition, PPP1R1B, CRKRS and TCAP are related to drug resistance and adverse reactions in the treatment of Her2 + BC.
Novel Water-Soluble Photosensitizer for Photodynamic Inactivation of Gram-Positive Bacteria Using Nontoxic Dose of UV Light
Authors: Yang Zihuayuan , Qiao Ying , Li Junying , Wu Fu-Gen , Lin Fengming 
Published: May 2020

Abstract
Background Antimicrobial photodynamic therapy (APDT) is a promising alternative to traditional antibiotics for bacterial infections, which inactivates a broad spectrum of bacteria. However, it has some disadvantages including poor water solubility and easy aggregation of hydrophobic photosensitizers (PS), and poor tissue penetration and cytotoxicity when using UV as the light source, leading to undesired photodynamic therapy efficacy.Results In this study, we develop a novel water-soluble natural PS (sorbicillinoids) obtained by microbial fermentation using recombinant filamentous fungus Trichoderma reesei (T. reesei). Sorbicillinoids could effectively generate singlet oxygen (1O2) under ultraviolet (UV) light irradiation, and ultimately display photoinactivation activity on Gram-positive bacteria, but not Gram-negative ones. Staphylococcus aureus (S. aureus) treated with sorbicillinoids and UV light displayed high levels of intracellular reactive oxygen species (ROS), notable DNA photocleavage, and compromised cell semi-permeability without overt cell membrane disruption. Moreover, the dark toxicity, phototoxicity or hemolysis activity of sorbicillinoids is negligible, showing its excellent biocompatibility.Conclusion Sorbicillinoids obtained from T. reesei display photoinactivation activity on Gram-positive bacteria using nontoxic dose of UV light irradiation and have an excellent biocompatibility Therefore, sorbicillinoids, a type of secondary metabolite from fungus, has a promising future as a new PS for APDT.
Masticatory Function In Elderly Compared To Young Adults
Authors: Ramos Verônica , Silva Anderson , Picinato-Pirola Melissa 
Published: May 2020

Abstract
Background

The performance of masticatory function in the elderly tends to be reduced due to dental absences, reduction of tone and muscle strength and incoordination of the orofacial musculature.
Aims

To characterize the masticatory function of the elderly and to compare total amount of time, masticatory strokes and total mastication score among the elderly and young adults.
Methods

A total of 50 individuals participated, 25 elderly (mean age 66 years) and 25 young adults (mean age 22 years). The evaluation of mastication was performed by standardized filming of the usual mastication of a wheat flour biscuit. The masticatory type (alternated bilateral, simultaneous bilateral, preferential unilateral, chronic and anterior), masticatory score, total masticatory time and the total number of masticatory strokes were verified and compared between the elderly and young adults.
Results

The predominant masticatory pattern in the young adults was the alternated bilateral mastication (52%), while, in the elderly, the simultaneous bilateral mastication predominated (48%). The use of dental prostheses had a significant influence on the total mastication score; elderly presented greater masticatory time and greater amount of masticatory strokes; however, the total masticatory score was lower for this group.
Conclusions

The use of dental prosthesis has a significant influence on masticatory function. The elderly had a greater amount of time and masticatory strokes and a lower total mastication score.
Identification and characteristics of postnatal development-related long non-coding RNAs under microbiota dependent condition
Authors: Zheng Miaomiao , Zhang Binbin , Zhang Yidan , Sun Tingting , Hu Baozhong 
Published: May 2020

Abstract
BackgroundThe interplay of long-non coding RNAs (lncRNAs) and the intestinal microbiota may serve as an essential role in intestinal development and homeostasis. Microbiota could regulate a large numbers of lncRNAs expression in intestinal epithelial cells. However, the associations between lncRNAs and microbiota during early postnatal development stages are still need to understand. MethodsIn present study, the microbial effects on lncRNA of intestinal epithelial cells (IECs) during postnatal development stage were investigated. ResultsWe identified gut microbiota-specific lncRNAs in diverse postnatal development stages including week 1, week 4 and week 12/16 of mice. A large proportion of gut microbiota-specific lncRNAs only were differential expressed in a single postnatal development stage. Up- and down-regulated gut microbiota-specific lncRNAs both showed consistent expression pattern. We also constructed gut microbiota-specific lncRNAs and coding genes interacted co-expressed networks. Functional analysis indicated that gut microbiota-specific lncRNAs were associated with ABC transporters. ConclusionsIn summary, the present study characterizes the landscape of lncRNAs associated with gut microbiota in different postnatal development stages. It provide assistance for exploring the relationships among lncRNAs, gut microbiota and postnatal development stages.
Inhibiting roles of FOXA2 in hepatocellular carcinoma cell migration and invasion by transcriptionally suppressing microRNA-103a-3p and activating the GREM2/LATS2 axis
Authors: Ma Guangzhen , Chen Jirong , Wei Tiantian , Wang Jia , Chen Wenshan 
Published: May 2020

Abstract
Background Forkhead box A2 (FOXA2) is a transcriptional activator for liver-specific genes. Hepatocellular carcinoma (HCC) is a prevalent fetal malignancy across the globe. This work focused on the role of FOXA2 in HCC cell migration and invasion and the involving molecules. Methods FOXA2 expression in HCC tissues and cells was determined using RT-qPCR. Altered expression of FOXA2 was introduced to identify its role in HCC cell migration and invasion using Transwell assays. The potential target microRNA (miRNA) of FOXA2 was predicted via online prediction and validated through a ChIP assay, and the mRNA target of miRNA-103a-3p was predicted and confirmed through a luciferase assay. The roles of miR-103a-3p and GREM2 in HCC cell invasion and migration were determined, and the downstream molecules mediated by GREM2 were analyzed. Results FOXA2 and GREM2 were poorly expressed while miR-103a-3p was abundant in HCC tissues and cells. Overexpression of FOXA2 or GREM2 suppressed migration and invasion of HepG2 and SK-HEP-1 cells, while up-regulation of miR-103a-3p led to reverse trends. FOXA2 transcriptionally suppressed miR-103a-3p to increase GREM2 expression, and silencing of GREM2 partially blocked the inhibitory effects of FOXA2 on cell migration and invasion. GREM2 increased LATS2 activity and YAP phosphorylation and degradation. Conclusion This study evidenced that FOXA2 inhibits migration and invasion potentials of HCC cell lines through suppressing miR-103a-3p transcription. The following upregulation of GREM2 plays key roles in migration inhibition by promoting LATS2 activity and YAP phosphorylation. This study may offer new insights into HCC treatment.
Alcohol drinking as a mediator of the influence of smoking prevalence on second-hand smoke exposure in workplaces: A mediation analysis
Authors: LIN Haoxiang , Chang Chun , LIU Zhao 
Published: May 2020

Abstract
Objective: Both alcohol drinking and second-hand smoke (SHS) exposure have shown a strong association with smoking behaviour. This study uses mediation analysis to clarify the process underlying the relationship between smoking prevalence and SHS exposure and the extent to which this relationship is mediated by alcohol use in the workplace.Method: A total of 14195 employees from 81 companies in China participated in this survey. Mediation analysis was used to establish the mediation effect of alcohol drinking. Structural equation modelling（SEM） was used to evaluate the role of alcohol drinking when a smoke-free (SF) workplace policy was imposed.Results: For males, approximately 6.3% of the effects on SHS exposure were mediated through the channel of alcohol use. For females, this channel mediated 14.1% of the total effects. SF policy was negatively associated with smoking prevalence and SHS exposure. The indirect effect of the SF policy on reducing SHS exposure by controlling smoking behaviour was significant. For smokers, worksite smoking bans were negatively associated with the smoking amount and drinking behaviour, and the indirect effect of this policy on promoting smokers’ intention to quit by reducing the smoking amount and controlling drinking behaviour was significant.Conclusion: This study confirmed that alcohol drinking is among the channels that mediate the association between smoking prevalence and SHS exposure in workplaces. Our results also find a spillover effect of SF workplace policy and call for effective interventions for alcohol use, which may influence the outcomes of SF policy implementation.
Price behavior of Turmeric in Sangali APMC
Authors: KOLEKAR PALLAVI LALASAHEB , shinde reshma , Shinde Shital Pravin 
Published: May 2020

Abstract
Prices of Agricultural commodity fluctuates time to time, in Sangali turmeric is one of the major crop grown, the prices of Turmeric are inversely related with arrivals. After harvesting arrivals are more in AMPC And prices are less. Seasonal indices and growth rate are used for study purpose.
Disruption of the CCL5/RANTES-CCR5 Pathway Restores Immune Homeostasis and Reduces Plasma Viral Load in Critical COVID-19
Authors: Pattterson Bruce , Seetthamraju Harish , Dhody Kush , Corley Michael , Kazempour Kazem , Lalezari Jay , Pang Alina , Sugai Christopher , Francisco Edgar , Pise Amruta , Rodrigues Hallison , Ryou Matthew , Wu Helen , Webb Gabriela , Park Byung , Kelly Scott , Pourhassan Nader , Lelic Alena , Kdouh Lama , Herrera Monica , Hall Eric , Aklin Enver , Ndhlovu Lishomwa , Sacha Jonah 
Published: May 2020

Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of coronavirus disease 2019 (COVID-19), is now pandemic with nearly three million cases reported to date1. Although the majority of COVID-19 patients experience only mild or moderate symptoms, a subset will progress to severe disease with pneumonia and acute respiratory distress syndrome (ARDS) requiring mechanical ventilation2. Emerging results indicate a dysregulated immune response characterized by runaway inflammation, including cytokine release syndrome (CRS), as the major driver of pathology in severe COVID-193,4. With no treatments currently approved for COVID-19, therapeutics to prevent or treat the excessive inflammation in severe disease caused by SARS-CoV-2 infection are urgently needed. Here, in 10 terminally-ill, critical COVID-19 patients we report profound elevation of plasma IL-6 and CCL5 (RANTES), decreased CD8+ T cell levels, and SARS-CoV-2 plasma viremia. Following compassionate care treatment with the CCR5 blocking antibody leronlimab, we observed complete CCR5 receptor occupancy on macrophage and T cells, rapid reduction of plasma IL-6, restoration of the CD4/CD8 ratio, and a significant decrease in SARS-CoV-2 plasma viremia. Consistent with reduction of plasma IL-6, single-cell RNA-sequencing revealed declines in transcriptomic myeloid cell clusters expressing IL-6 and interferon-related genes. These results demonstrate a novel approach to resolving unchecked inflammation, restoring immunologic deficiencies, and reducing SARS-CoV-2 plasma viral load via disruption of the CCL5-CCR5 axis, and support randomized clinical trials to assess clinical efficacy of leronlimab-mediated inhibition of CCR5 for COVID-19.
Anosmia, a Hidden Sign for COVID-19? A Case Report and Literature Review
Authors: Christelle Karen , Zulkifli Maryam Mohd , Draman Nani 
Published: May 2020

Abstract
Background The coronavirus disease 2019 (COVID-19) is an ongoing viral pandemic that is actively affecting 210 countries worldwide, with a total of more than 1.5 million cases and 106 000 deaths. Symptoms associated with COVID-19 are mainly fever, cough, dyspnea and sore throat. The current indication for COVID-19 testing includes presence of these symptoms with a positive history of travel to affected countries or contact with COVID-19 patients. Anosmia has been recently reported anecdotally over the past weeks as an emerging symptom of the COVID-19 but has yet to gain recognition as a symptom for COVID-19 by the World Health Organization (WHO) and Centre for Disease Control and Prevention (CDC). This case report highlights a case of isolated sudden onset of anosmia as a presenting symptom of COVID-19 and relevant literature review supporting the incidence of anosmia in COVID-19. This is a first case report of anosmia in COVID-19 occurring in pregnancy.Case Presentation A 30-year-old pregnant lady at 11 weeks of gestation presented with sudden onset of anosmia for one day with no other accompanying symptoms. She had just recovered from a mild cold a day prior to the development of anosmia. She had a history of travel by land to Singapore 14 days prior to onset of anosmia. There was no known close contact with a COVID-19 patient or attended any mass gatherings prior to development of her symptom. She underwent nasopharyngeal and oropharyngeal swab sampling which was then tested using reverse transcription polymerase chain reaction (RT-PCR) method and confirmed infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).Conclusion Clinicians should be aware regarding anosmia as a presenting symptom of COVID-19 especially in the presence of risk factors such as travel to affected countries and having close contact with COVID-19 positive patients. If testing is not done, these patients should be advised for home quarantine to reduce the risk of transmission. Healthcare workers must always adhere to infection control and prevention protocol as well as personal protective equipment.
Inhibition of papain-like protease PLpro blocks SARS-CoV-2 spread and promotes anti-viral immunity
Authors: Shin Donghyuk , Mukherjee Rukmini , Grewe Diana , Bojkova Denisa , Baek Kheewoong , Bhattacharya Anshu , Schulz Laura , Widera Marek , Mehdipour Ahmad Reza , Tascher Georg , Geurink Paul P. , Noort Gerbrand J. van der Heden van , Ovaa Huib , Knobeloch Klaus- Peter , Rajalingam Krishnaraj , Schulman Brenda A. , Cinatl Jindrich , Hummer Gerhard , Ciesek Sandra , Dikic Ivan 
Published: May 2020

Abstract
Main protease and papain-like protease (PLpro) are essential coronaviral enzymes required for polypeptide processing during viral maturation. PLpro additionally cleaves proteinous post-translational modifications from host proteins to evade anti-viral immune responses. Here, we provide biochemical, structural and functional characterizations of PLpro from SARS-CoV-2 (PLproCoV2) and reveal differences to that of SARS (PLproSARS) in controlling interferon (IFN) and NF-kB pathways. PLproCoV2 and PLproSARS share 83% sequence identity, yet they differ in their host substrate preferences: PLproCoV2 predominantly cleaves the ubiquitin-like protein ISG15 off from host proteins, while PLproSARS preferentially targets ubiquitin chains. The crystal structure of PLproCoV2 in complex with ISG15 explains the affinity and higher specificity through distinctive binding to ISG15’s unique amino-terminal ubiquitin-like domain, and enabled the identification of GRL-0617 as a non-covalent candidate inhibitor for PLproCoV2. In human cells, PLproCoV2 cleaves ISG15 from interferon responsive factor 3 (IRF3), blocks its nuclear translocation, and reduces type I interferon responses, whereas PLproSARS preferentially mediates deubiquitination of critical components of the NF-kB pathway. Pharmacological inhibition of PLproCoV2 blocks the virus-induced cytopathogenic effect upon infection with SARS-CoV-2, fosters the anti- viral interferon pathway and reduces viral release from infected cells. We propose that therapeutic targeting of PLproCoV2 can suppress SARS-CoV-2 infection and promote anti-viral immunity.
DELAÇÃO PREMIADA: O DESAFIO DA PONDERAÇÃO ENTRE A NECESSIDADE DE SE COMBATER O CRIME ORGANIZADO E A OBSERVÂNCIA DOS DIREITOS E GARANTIAS FUNDAMENTAIS DO ACUSADO
Authors: SILVA Lívia Maria Lopes e , BARBOSA André Luis Jardini 
Published: May 2020

O objetivo geral deste trabalho foi analisar a pertinência da Delação Premiada e os pressupostos para sua introdução na legislação pátria; a percepção da mesma pela sociedade; e as consequências do uso contemporâneo do instituto, com foco na questão da (in) compatibilidade da mesma com os direitos e garantias fundamentais dos acusados, verificando até que ponto isto é tolerável. A modernização da sociedade, com a evolução dos meios de comunicação e informação, o fenômeno da globalização, entre outros fatores, acarretou também, inevitavelmente, a sofisticação do crime. Diante desse cenário, a Delação Premiada se mostra como um instrumento eficaz no combate ao crime organizado. No entanto, o procedimento utilizado parece ir de encontro a certas garantias constitucionais dos acusados.
Anomalies, a mod 2 index, and dynamics of 2d adjoint QCD
Authors: Cherman Aleksey , Jacobson Theo , Tanizaki Yuya , Ünsal Mithat 
Published: May 2020

We show that 22d
adjoint QCD, an SU(N)SU(N)
gauge theory with one massless adjoint Majorana fermion, has a variety
of mixed ’t Hooft anomalies. The anomalies are derived using a recent
mod 22
index theorem and its generalization that incorporates ’t Hooft flux.
Anomaly matching and dynamical considerations are used to determine the
ground-state structure of the theory. The anomalies, which are present
for most values of NN,
are matched by spontaneous chiral symmetry breaking. We find that
massless 22d
adjoint QCD confines for N >2N>2,
except for test charges of NN-ality
N/2N/2,
which are deconfined. In other words, \mathbb Z_NℤN
center symmetry is unbroken for odd NN
and spontaneously broken to \mathbb Z_{N/2}ℤN/2
for even NN.
All of these results are confirmed by explicit calculations on small
\mathbb{R}\times S^1ℝ×S1.
We also show that this non-supersymmetric theory exhibits exact
Bose-Fermi degeneracies for all states, including the vacua, when
NN
is even. Furthermore, for most values of NN,
22d
massive adjoint QCD describes a non-trivial symmetry-protected
topological (SPT) phase of matter, including certain cases where the
number of interacting Majorana fermions is a multiple of
88.
As a result, it fits into the classification of
(1+1)(1+1)d
SPT phases of interacting Majorana fermions in an interesting way.
HIBRIDIZAÇÃO CULTURAL E PAISAGENS SONORAS: INFLUÊNCIAS NA FORMAÇÃO DE JOVENS MÚSICOS
Authors: Bezerra Silvio Gleisson , Tauk Santos Maria Salett 
Published: May 2020

O objetivo desta investigação foi analisar o trabalho de formação de jovens músicos, desenvolvido pela Sociedade Musical 15 de Agosto na cidade de Aliança, Zona da Mata de Pernambuco, à luz da teoria da hibridização cultural de Canclini (2015) e dos estudos das paisagens sonoras de Schafer (2011). Desse modo, buscou-se compreender como as hibridizações se fazem presentes no processo de educação não formal de jovens, colaborando à construção de uma produção musical que faz parte de uma paisagem sonora local contemporânea sob as influências da cultura massiva. A investigação de caráter qualitativo, fundamentou-se na combinação de técnicas como a pesquisa bibliográfica, a análise documental, a observação direta com a utilização de diário de campo e a realização de entrevistas semiestruturadas com os sujeitos da pesquisa. Através da interpretação dos dados à luz das teorias escolhidas como indicadores, foram observados indícios de uma produção musical hibridizada, tendo os jovens como protagonistas do processo de formação musical. Além disso, os participantes da entidade identificaram através dos depoimentos, a presença de uma paisagem sonora híbrida, como parte de uma diversidade das culturas populares que comportam elementos da região e da cultura de massa transnacional.
A nonparametric statistical framework using a kernel density estimator to approximate flood marginal distributions – a case study for the Kelantan River Basin in Malaysia
Authors: Latif Shahid , Mustafa Firuza 
Published: May 2020

AbstractFloods are becoming the most challenging hydrologic issue in the Kelantan River basin in Malaysia. All three flood characteristics, i.e. peak flow, flood volume and flood duration, are important when formulating actions and measures to manage flood risk. Therefore, estimating the multivariate designs and their associated return periods is an essential element of making informed risk-based decisions in this river basin. In this paper, the efficacy of a kernel density estimator is tested by assessing the adequacy of kernel functions for capturing flood marginal density of 50 years (from 1961 to 2016) of daily streamflow data collected at Gulliemard Bridge gauge station in the Kelantan River basin. Tests for stationarity or the existence of serial correlation within the flood series is often a pre-requisite before introducing the random samples into a univariate or a multivariate framework. It was found that homogeneity existed within the flood vector series. It was concluded therefore that time series of the flood vectors do not exhibit any significant trend. Based on analytically based fitness measures, it was concluded that it is likely that Triweight kernel function is the best-fitted distribution for defining the marginal distribution of peak flows, flood volumes and flood durations in the Kelantan River basin.