bluuebunny/crossref_metadata_2025_split huggingface dataset:
Stability of serum precipitins to Aspergillus fumigatus for the diagnosis of allergic bronchopulmonary aspergillosis
Authors: Lau Kevin S. K. , Yeung Chantane , Carlsten Chris 
Published: September 2020

Abstract
Background
Allergic bronchopulmonary aspergillosis (ABPA) reflects hypersensitivity and an exaggerated immune response to Aspergillus fumigatus. ABPA typically occurs in individuals with airway diseases such as asthma or cystic fibrosis and is associated with worse outcomes for individuals with these conditions. Each year, physicians across the province of British Columbia submit over 2600 diagnostic testing requests to a centralized location in Vancouver, requiring specimen collection, storage, and shipment from different clinics across the province. Timely and reliable testing of Aspergillus precipitins is critical to optimizing diagnosis and management of ABPA. At our centre, we analyzed sample stability in varying storage conditions to provide guidance to those using this routine diagnostic test.

Methods
To determine temperature and time stability, 31 serum specimens positive for Aspergillus fumigatus precipitins from routine clinical testing were each aliquoted and incubated at 4 and 37 °C. Samples were repeatedly assayed for precipitins to Aspergillus fumigatus via agarose gel double immunodiffusion (AGID) at 7, 14, and 28 days post-incubation.
To determine freeze–thaw stability, 39 serum specimens submitted for routine clinical testing for Aspergillus precipitins were randomly selected. Each specimen was aliquoted and stored at 4 or −20 °C. 4 °C samples were maintained at 4 °C while −20 °C samples were split into three groups corresponding to one, two, or three freeze–thaw cycles. −20 °C samples were thawed at room temperature in the morning and then immediately frozen overnight for up to a total of three freeze–thaw cycles.

Results
Regarding temperature and time stability, median stability time was 47 and 34 days at 4 and 37 °C, respectively. The log-rank model indicates no statistically significant difference between the two temperature storage conditions (p = 0.14) with a hazard ratio of 0.61 (95% CI, 0.31–1.2).
In terms of freeze–thaw stability, no indication of serum degradation with regards to Aspergillus fumigatus precipitins was found with repeated freeze–thaw cycles as compared to refrigerated storage.

Conclusions
The stability of serum precipitins to Aspergillus fumigatus was found to be dependent on time, but not temperature and freeze–thaw cycles. Specimens for Aspergillus fumigatus precipitins testing should be shipped at ambient temperature and tested within 2 weeks from collection.
The anti-fibrotic effect of human fetal skin-derived stem cell secretome on the liver fibrosis
Authors: Yao Xia , Wang Jing , Zhu Jiajing , Rong Xiaoli 
Published: September 2020

Abstract
Background
Liver fibrosis resulting from chronic liver injury is one of the major causes of mortality worldwide. Stem cell-secreted secretome has been evaluated for overcoming the limitations of cell-based therapy in hepatic disease, while maintaining its advantages.

Methods
In this study, we investigated the effect of human fetal skin-derived stem cell (hFSSC) secretome in the treatment of liver fibrosis. To determine the therapeutic potential of the hFSSC secretome in liver fibrosis, we established the CCl4-induced rat liver fibrosis model and administered hFSSC secretome in vivo. Moreover, we investigated the anti-fibrotic mechanism of hFSSC secretome in hepatic stellate cells (HSCs).

Results
Our results showed that hFSSC secretome effectively reduced collagen content in liver, improved the liver function and promoted liver regeneration. Interestingly, we also found that hFSSC secretome reduced liver fibrosis through suppressing the epithelial-mesenchymal transition (EMT) process. In addition, we found that hFSSC secretome inhibited the TGF-β1, Smad2, Smad3, and Collagen I expression, however, increased the Smad7 expression.

Conclusions
In conclusions, our results suggest that hFSSC secretome treatment could reduce CCl4-induced liver fibrosis via regulating the TGF-β/Smad signal pathway.
Mesenchymal stem cell-based cell-free strategies: safe and effective treatments for liver injury
Authors: Hu Chenxia , Zhao Lingfei , Zhang Lingjian , Bao Qiongling , Li Lanjuan 
Published: September 2020

AbstractVarious hepatoxic factors, such as viruses, drugs, lipid deposition, and autoimmune responses, induce acute or chronic liver injury, and 3.5% of all worldwide deaths result from liver cirrhosis, liver failure, or hepatocellular carcinoma. Liver transplantation is currently limited by few liver donors, expensive surgical costs, and severe immune rejection. Cell therapy, including hepatocyte transplantation and stem cell transplantation, has recently become an attractive option to reduce the overall need for liver transplantation and reduce the wait time for patients. Recent studies showed that mesenchymal stem cell (MSC) administration was a promising therapeutic approach for promoting liver regeneration and repairing liver injury by the migration of cells into liver sites, hepatogenic differentiation, immunoregulation, and paracrine mechanisms. MSCs secrete a large number of molecules into the extracellular space, and soluble proteins, free nucleic acids, lipids, and extracellular vesicles (EVs) effectively repair tissue injury in response to fluctuations in physiological states or pathological conditions. Cell-free-based therapies avoid the potential tumorigenicity, rejection of cells, emboli formation, undesired differentiation, and infection transmission of MSC transplantation. In this review, we focus on the potential mechanisms of MSC-based cell-free strategies for attenuating liver injury in various liver diseases. Secretome-mediated paracrine effects participate in the regulation of the hepatic immune microenvironment and promotion of hepatic epithelial repair. We look forward to completely reversing liver injury through an MSC-based cell-free strategy in regenerative medicine in the near future.
Quality and best practice in medical laboratories: specific requests for autoimmunity testing
Authors: Sack Ulrich , Bossuyt Xavier , Andreeva Hristina , Antal-Szalmás Péter , Bizzaro Nicola , Bogdanos Dimitrios , Borzova Elena , Conrad Karsten , Dragon-Durey Marie-Agnes , Eriksson Catharina , Fischer Katarzyna , Haapala Anna-Maija , Heijnen Ingmar , Herold Manfred , Klotz Werner , Kozmar Ana , Tesija Kuna Andrea , López Hoyos Marcos , Malkov Vladimir A. , Musset Lucile , Nagy Eszter , Rönnelid Johan , Shoenfeld Yehuda , Sundic Tatjana , Tsirogianni Alexandra , Uibo Raivo , Rego Sousa Maria José , Damoiseaux Jan ,   on behalf of the European Autoimmunity Standardisation Initiative
Published: September 2020

AbstractSpecial conditions associated with laboratory autoimmune testing are not well compatible with recent developments in regulatory frameworks such as EN/ISO 15189 accreditation or in vitro diagnostic medical device regulation (IVD-R). In addition, international recommendations, guidelines and disease criteria are poorly defined with respect to requirements on autoantibody testing. Laboratory specialists from Austria, Belgium, Croatia, Estonia, Finland, France, Germany, Greece, Hungary, Italy, Norway, Poland, Portugal, South Africa, Spain, Sweden, Switzerland, and The Netherlands collected information, reported national experience, and identified quality issues in relation to autoantibody testing that require consensus on interpretation of the regulatory frameworks and guidelines. This process has been organized by the European Autoimmunity Standardisation Initiative (EASI). By identifying the critical items and looking for a consensus, our objective was to define a framework for, in particular, EN/ISO accreditation purposes. Here, we present a review of current publications and guidelines in this field to unify national guidelines and deliver in this way a European handout on quality control and accreditation for laboratories involved in autoantibody testing. We focus on quality items that can be checked during accreditation visits. Despite various local varieties, we encountered an overwhelming dedication to quality assurance in all contributing countries.
Canine Parvovirus is diagnosed and neutralized by chicken IgY-scFv generated against the virus capsid protein
Authors: Ge Shikun , Xu Long , Li Ben , Zhong Fagang , Liu Xiang , Zhang Xiaoying 
Published: September 2020

AbstractCanine parvovirus (CPV) can cause acute and highly contagious bloody enteritis in dog. To obtain antibodies against CPV, hens were immunized with virus-like particles (VLP) of CPV-VP2. The IgY single chain fragment variables (scFv) were generated by T7 phage display system and expressed in E. coli system. The titer of the primary scFv library reached to 1.5 × 106 pfu/mL, and 95% of the phages contained the target fragments. The CPV-VLP and CPV-VP2 protein showed similar reaction values to the purified scFv in the ELISA test, and the results of ELISA analysis using IgY-scFv toward CPV clinical samples were consistent with commercial immunochromatographic assay (ICA) and PCR detection, the scFv did not show cross reactivity with canine distemper virus (CDV) and canine coronavirus (CCV). IgY-scFv was successfully expressed in CRFK cells, and in the virus suppression assay, 55% of CPV infections were eliminated within 24 h. Docking results demonstrated that the number of amino acids of the binding sides between scFv and VP2 were AA37 and AA40, respectively. This study revealed the feasibility of a novel functional antibody fragment development strategy by generating diversified avian IgY-scFv libraries towards the pathogenic target of interest for both detection and therapeutic purposes in veterinary medicine.
On a fractional q-differential inclusion on a time scale via endpoints and numerical calculations
Authors: Samei Mohammad Esmael , Rezapour Shahram 
Published: September 2020

AbstractBy using an endpoint result for set-valued maps, we study the existence of solutions for a fractional q-differential inclusion with sum and integral boundary value conditions on the time scale $\mathbb{T}_{t_{0}}= \{t_{0} q, t_{0} q^{2},\ldots \} \cup \{0\}$

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, where $t_{0}$

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is a real number and $q \in (0,1)$
q
∈
(
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. We provide an example involving some graphs and algorithms via numerical calculations to illustrate our main result.
On weak and strong convergence results for generalized equilibrium variational inclusion problems in Hilbert spaces
Authors: Rezapour Shahram , Zakeri Seyyed Hasan 
Published: September 2020

AbstractWe introduce a new iterative method for finding a common element of the set of fixed points of pseudo-contractive mapping, the set of solutions to a variational inclusion and the set of solutions to a generalized equilibrium problem in a real Hilbert space. We provide some results about strongly and weakly convergent of the iterative scheme sequence to a point $p\in \varOmega $
p
∈
Ω
which is the unique solution of a variational inequality, where Ω is an intersection of set as given by ${\varOmega }=F(S)\cap (A+B)^{-1}(0) \cap N^{-1}(0)\cap \operatorname{GEP}(F,M)\neq \emptyset $
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. This gives us a common solution. Also, We show that our results extend some published recent results in this field. Finally, we provide an example to illustrate our main result.
Extreme geomagnetic activities: a statistical study
Authors: Kataoka Ryuho 
Published: September 2020

AbstractStatistical distributions are investigated for magnetic storms, sudden commencements (SCs), and substorms to identify the possible amplitude of the one in 100-year and 1000-year events from a limited data set of less than 100 years. The lists of magnetic storms and SCs are provided from Kakioka Magnetic Observatory, while the lists of substorms are obtained from SuperMAG. It is found that majorities of events essentially follow the log-normal distribution, as expected from the random output from a complex system. However, it is uncertain that large-amplitude events follow the same log-normal distributions, and rather follow the power-law distributions. Based on the statistical distributions, the probable amplitudes of the 100-year (1000-year) events can be estimated for magnetic storms, SCs, and substorms as approximately 750 nT (1100 nT), 230 nT (450 nT), and 5000 nT (6200 nT), respectively. The possible origin to cause the statistical distributions is also discussed, consulting the other space weather phenomena such as solar flares, coronal mass ejections, and solar energetic particles.
Severe proteinuria during the administration of bevacizumab plus mFOLFOX6 in a colorectal cancer patient after kidney transplantation: a case report
Authors: Onodera Ren , Nihei Satoru , Kimura Toshimoto , Tomita Takashi , Kudo Kenzo 
Published: September 2020

Abstract
Background
Bevacizumab (BEV) leads to proteinuria and renal damage. It is not clear whether the administration of immunosuppressive drugs after renal transplantation affects the safety of BEV administration. We report a case of severe proteinuria caused by BEV plus 5-fluorouracil, levofolinate, and oxaliplatin (mFOLFOX6) in a patient who had previously undergone kidney transplantation and the administration of tacrolimus.

Case presentation
The patient was a 67-year-old man with a history of diabetes and hypertension. He developed chronic renal failure 14 years earlier and underwent right kidney transplantation from a living donor followed by the administration of tacrolimus and mycophenolate mofetil for immunosuppression. After kidney transplantation, the patient was diagnosed with colorectal cancer with multiple lung and liver metastases and received BEV plus mFOLFOX6. After 5 cycles, proteinuria was observed, with a urinary protein concentration of > 300 mg/dL (urine protein creatinine ratio: 3.5), and after 16 cycles, the urinary protein concentration was > 1000 mg/dL (urine protein creatinine ratio: 7.1). Subsequently, BEV was discontinued, and only mFOLFOX6 administration was continued. Tacrolimus continued to be administered during chemotherapy. There was no association between serum tacrolimus concentration and proteinuria.

Conclusions
In this case, BEV administration caused severe proteinuria without affecting blood levels of tacrolimus. Patients with risk factors for renal impairment should be carefully evaluated for the risks and benefits of BEV administration.
Cardiometabolic health during early adulthood and risk of miscarriage: a prospective study
Authors: Magnus Maria C. , Ferreira Diana D. S. , Borges Maria Carolina , Tilling Kate , Lawlor Deborah A. , Fraser Abigail 
Published: September 2020

Background: Several studies have found that women who are overweight or obese have an increased risk of miscarriage. There is also some evidence of associations of other aspects of cardiometabolic health, including blood pressure and lipids, with miscarriage risk, although these have not been examined to the same extent as body-mass index (BMI). Methods: Our objective was to investigate the risk of miscarriage according to pre-pregnancy cardiometabolic health. We examined pre-pregnancy levels of BMI, blood pressure, fasting insulin and metabolites profile at age 18 and risk of miscarriage by age 24. The study included adult female offspring in the Avon Longitudinal Study of Parents and Children with a pregnancy between 18 and 24 years of age (n=434 for BMI and blood pressure; n=265 for metabolites). We used log-binomial regression to calculate adjusted associations between cardiometabolic health measures and miscarriage. Results: The overall risk of miscarriage was 22%. The adjusted relative risks for miscarriage were 0.96 (95% CI: 0.92-1.00) for BMI (per unit increase), 0.98 (0.96-1.00) for systolic blood pressure, and 1.00 (0.97-1.04) for diastolic blood pressure (per 1 mmHg increase). Total cholesterol, total lipids and phospholipids in HDL-cholesterol were associated with increased likelihood of miscarriage, but none of the p-values for the metabolites were below the corrected threshold for multiple testing (p-value ≤0.003). Conclusions: Pre-pregnancy cardiometabolic health in late adolescence was not associated with miscarriage risk in young, healthy women who became pregnant before age 24.
LEADER ENDORSEMENT: THE ROLE OF LEADER PROTOTYPICALITY, LEADER EFFECTIVENESS AND EMPLOYEE’S ORGANIZATIONAL IDENTIFICATION
Authors: Rochman Taufik Nur , Samian Samian , Riantoputra Corina D. 
Published: September 2020

Leadership occurs when the individuals claimed capable of leading, received endorsements from their subordinates. Without endorsements (i.e., subordinates’ willingness to support and be directed by the leader), no leader will be able to direct and influence their subordinates. Using the Social Identity Theory of leadership, this research aimed to investigate the factors influencing leader endorsement. Through an online survey with good internal consistency (i.e., coefficient reliability ranging from .7 to .9), this study was able to collect data from 186 private employees across Indonesia. The moderated multiple regression analysis showed 1) subordinates tend to endorse prototypical leaders; 2) organizational identification is positively related to leader endorsement; and 3) leader effectiveness weakens the positive relationship between leader prototypicality and leader endorsement. This study contributes to the social identity theory of leadership by demonstrating that low prototypical leaders may still get endorsed, as long as they are showing effective behavior. This new finding sheds light on what kind of leadership behaviors may win the endorsement from their subordinates.
THE ROLE OF MINDFUL PARENTING TO THE PARENTING STRESS IN MOTHERS WITH CHILDREN AT EARLY AGE
Authors: Kumalasari Dewi , Fourianalistyawati Endang 
Published: September 2020

Parenting is an important process, and the processes in each phase are challenging, particularly during the phase of early childhood. The challenges in parenting could trigger parenting stress. The ability to give full attention to the conducted parenting process can help parents in coping the parental stress. The process of giving full attention to parenting activities is known as the mindful parenting concept. This study aims to determine the role of mindful parenting to parenting stress of mothers with a child at an early age. This study used a quantitative method. A Mindfulness in Parenting Questionnaire (28 items, α = .915) and Parental Stress Scale (18 items, α = .705) were given to the respondents. The data was collected from 281 respondents (ages 21-54 years, M = 32.75, SD = 5), which was selected through incidental sampling methods. Based on the regression analysis, the results show that mindful parenting has a significant contribution to the low parenting stress level in mothers with child at early age (R = -.260, R2 = .068, β = -0.119, F (1, 280) = 20.153, p < 0.01). This result suggests that mindful parenting skills can be an alternative way to reduce parental stress on mothers with a child in early childhood.
Distinct Corticospinal and Reticulospinal Contributions to Voluntary Control of Elbow Flexor and Extensor Muscles in Humans with Tetraplegia
Authors: Sangari Sina , Perez Monica A. 
Published: September 2020

Humans with cervical spinal cord injury (SCI) often recover voluntary control of elbow flexors and, to a much lesser extent, elbow extensor muscles. The neural mechanisms underlying this asymmetrical recovery remain unknown. Anatomical and physiological evidence in animals and humans indicates that corticospinal and reticulospinal pathways differentially control elbow flexor and extensor motoneurons; therefore, it is possible that reorganization in these pathways contributes to the asymmetrical recovery of elbow muscles after SCI. To test this hypothesis, we examined motor-evoked potentials (MEPs) elicited by transcranial magnetic stimulation over the arm representation of the primary motor cortex, maximal voluntary contractions, the StartReact response (a shortening in reaction time evoked by a startling stimulus), and the effect of an acoustic startle cue on MEPs elicited by cervicomedullary stimulation (CMEPs) on biceps and triceps brachii in males and females with and without chronic cervical incomplete SCI. We found that SCI participants showed similar MEPs and maximal voluntary contractions in biceps but smaller responses in triceps compared with controls, suggesting reduced corticospinal inputs to elbow extensors. The StartReact and CMEP facilitation was larger in biceps but similar to controls in triceps, suggesting enhanced reticulospinal inputs to elbow flexors. These findings support the hypothesis that the recovery of biceps after cervical SCI results, at least in part, from increased reticulospinal inputs and that the lack of these extra inputs combined with the loss of corticospinal drive contribute to the pronounced weakness found in triceps.SIGNIFICANCE STATEMENTAlthough a number of individuals with cervical incomplete spinal cord injury show limited functional recovery of elbow extensors compared with elbow flexor muscles, to date, the neural mechanisms underlying this asymmetrical recovery remain unknown. Here, we provide for the first time evidence for increased reticulospinal inputs to biceps but not triceps brachii and loss of corticospinal drive to triceps brachii in humans with tetraplegia. We propose that this reorganization in descending control contributes to the asymmetrical recovery between elbow flexor and extensor muscles after cervical spinal cord injury.
Los naufragios de Maqroll: alcances y límites de la conciencia trágica en la narrativa de Mutis
Authors: Cáceres Delgadillo Carolina 
Published: September 2020

Ante la paradoja trágica que implica para el hombre existir -vivir en la conciencia de los límites y la absoluta contingencia-,Maqroll opta por una forma particular de enfrentarse a ella y así, desarro llar una cosmovisión propia y coherente, que, de alguna manera, dirige sus actos. Lo trágico, entendido como valor simbólico inserto en el fundamento de la conciencia, no es una "contemplación indiferente, preocupada exclusivamente por entender los límites de la naturaleza humana" (Jaspers, 1960). Lo trágico, no sólo es un conocimiento racional o una reflexión abstracta, sino también un reconocimiento afectivo de lo otro, en el que se logra plenamente el ‘yo' en otras palabras, un reconocimiento en el cual el personaje de ficción deja de ser la encarnación de un estado puro de conciencia, para convertirse en reflejo de un ser humano en acto, con todas sus dimensiones (emocional, ética y espiritual).En estudios anteriores se ha hablado de esos valores externos (at­ mósfera, argumento y lenguaje), que acercan la creación artística de Alvaro Mutis a una manifestación de las formas trágicas, o a la estética de lo trágico; sin embargo, lo trágico va más lejos todavía: desborda la miseria fáctica a través de la sublimación intelectiva del personaje y su actitud frente a un mundo en continuo deterioro, no sólo es aceptación, sino también es lucha, culpa compartida, redención asimilada. Ahora bien, el interrogante que se intenta responder en el presente artículo es: ¿hasta dónde Maqroll, paradigma de la creación mutisiana, encarna una verdadera axiología trágica?