bluuebunny/crossref_metadata_2025_split huggingface dataset:
Elevated TRIM23 expression predicts cisplatin resistance in lung adenocarcinoma
Authors: Zhang Youwei , Du He , Li Yang , Yuan Yuan , Chen Bi , Sun Sanyuan 
Published: November 2019

AbstractThe tripartite motif containing 23 (TRIM23) gene is a member of the tripartite motif (TRIM) family that participates in many pathophysiological processes. However, the role of TRIM23 in lung adenocarcinoma (LUAD) remains unclear. In the present study, TRIM23 was first screened by next‐generation sequencing between the cisplatin (DDP)‐resistant A549/DDP cell line and the parental A549 cell line, combined with integrated analysis of the Gene Expression Omnibus (GEO) data (E‐GEOD‐43493 and E‐GEOD‐43494). The expression of TRIM23 was then verified to be upregulated in the DDP‐resistant LUAD cells and tissues. The knockdown of TRIM23 expression in A549/DDP cells caused increased apoptosis, decreased IC50 values of DDP, NF‐κB nuclear translocation, inhibition of cell proliferation in vitro and in vivo, inhibition of GLUT1/3 expression, glucose uptake, and lactate and ATP production. TRIM23 overexpression resulted in the opposite effects in A549 cells. In addition, the inhibition of proliferation in A549 cells caused by NF‐κB signaling inhibitor PTDC or glycolysis inhibitor 3‐BrPA could be weakened by TRIM23 overexpression. Furthermore, immunohistochemical analysis revealed that TRIM23 was upregulated in 46.1% (70/152) of LUAD cases, and elevated TRIM23 expression was correlated with high expression of NF‐κB, poor cellular differentiation, and adverse overall survival (OS) and disease‐free survival (DFS). In conclusion, our study demonstrates that TRIM23 acts as an oncogene in LUAD and promotes DDP resistance by regulating glucose metabolism via the TRIM23/NF‐κB/ GLUT1/3 axis.
Effect of clomiphene citrate treatment on the Sertoli cells of dysmetabolic obese men with low testosterone levels
Authors: Pelusi Carla , Fanelli Flaminia , Baccini Margherita , Triggiani Vincenzo , Bartolomeo Nicola , Carbone Matteo Domenico , De Pergola Giovanni , Di Dalmazi Guido , Pagotto Uberto , Pasquali Renato , Giagulli Vito Angelo 
Published: November 2019

AbstractBackgroundClomiphene citrate (CC) has been shown to restore the hypothalamic‐pituitary‐gonadal (HPG) axis by increasing testosterone (T) levels to physiological levels in patients with dysmetabolic conditions such as obesity, metabolic syndrome and type 2 diabetes mellitus (T2DM). However, the data are unclear regarding the effects on Sertoli cell (SC) function.AimTo study SC function by assessing Inhibin B (IB) and anti‐Mullerian hormone (AMH) levels at baseline and after 3 months of CC treatment.Materials and methodsThis is an ancillary study of a cross‐over, randomised, double‐blind, placebo‐controlled trial performed to evaluate androgen response to CC treatment in dysmetabolic obese subjects with low T levels treated with metformin. We evaluated SC function by assessing IB and AMH levels at baseline and after 3 months of each treatment in ten dysmetabolic obese subjects with low T levels. In all subjects, the influence of the clinical characteristics, metabolic and hormonal baseline parameters on SC and Leydig (LC) function, evaluated respectively with AMH, IB, follicle‐stimulating hormone (FSH) and T levels, was tested.ResultsNo significant changes were observed for IB and AMH concentrations after each treatment period. Whereas T and oestradiol (E2) levels were shown to be significantly higher in the CC plus metformin phase (CC/Met) only. No clinical, metabolic or hormonal parameters showed significant effects on serum AMH at baseline or after treatments. However, baseline T, dihydrotestosterone (DHT) and E2 positively affected IB levels during CC/Met therapy (P = .003, P = .038 and P = .049, respectively). Baseline leptin and FSH had a negative (P = 031) and positive (P = .048) respectively role on T levels during CC/Met, as they were statistically significant compared to the placebo period (Plac/Met).ConclusionUnlike the LC activity, CC was unable to influence SC function, as shown by the lack of IB and AMH serum modifications, thus suggesting an intrinsic nonreversible defect of SC cells in patients with dysmetabolic conditions.
Reduced perforin release from T cells as a mechanism underlying hypothermia‐mediated neuroprotection
Authors: Matsui Tomohiro , Yoshida Yusuke 
Published: November 2019

AbstractObjectiveThe underlying mechanisms of therapeutic hypothermia, which protects neurons after severe brain damage, are partially understood. T cells infiltrate the brain within days after cerebral ischemia and play essential roles in exacerbating the delayed phase of brain injury by producing cytotoxic factors, which were also systematically shown to be involved in brain damage. Periphery brain abnormalities are interesting, because the periphery might constitute a pathway to the central nervous system and therefore could also be a target of therapeutic hypothermia. We elucidated the effects of hypothermia and hyperthermia on peripheral T cell‐derived perforin (Pfn), and the underlying mechanism of Pfn‐mediated neurotoxicity and death of brain microvascular endothelial cells (BMVECs).MethodsWe determined the levels of Pfn produced by activated CD4+ and CD8+ T cells obtained from healthy humans under hypothermic, normothermic and hyperthermic conditions. The viability in response to Pfn treatment was assessed in neuronal Neuro‐2a and brain microvascular endothelial bEnd.3 cells.ResultsCompared with normothermia, Pfn release in both CD4+ and CD8+ T cells was reduced by hypothermia, but augmented by hyperthermia. Pfn caused the death of Neuro‐2a cells, and induced both apoptosis and necrosis in bEnd.3 cells; both effects were concentration‐dependent.ConclusionsHypothermia reduces T cell‐derived release of Pfn, which mediates neuronal cell death and apoptosis/necrosis of BMVECs, suggesting that therapeutic hypothermia elicits protection in the neurons and BMVECs in the delayed phase of brain injury by directly reducing the release of T cell‐derived Pfn. BMVECs protection can aid in reduced vascular permeability and blood–brain barrier protection.
Performance of bone‐level implants with conical connections in the anterior maxilla: A 5‐year prospective cohort study
Authors: Meijndert Caroliene M. , Raghoebar Gerry M. , Santing Hendrik J. , Vissink Arjan , Meijer Henny J. A. 
Published: November 2019

AbstractObjectivesTo assess clinical, radiographic and aesthetic outcomes, and satisfaction of patients treated with single implant restorations in the maxillary aesthetic region 5 years after final restoration.Material and methodsSixty patients (29 male/31 female, mean age 37 years) with a missing anterior tooth in the maxilla (39 central incisors/10 lateral incisors/5 cuspids/6 first premolars) received a bone‐level implant with conical connection. In 29 patients, a bone augmentation procedure was necessary before implant placement (autogenous bone grafts mixed with spongiosa granules). All implants (12 with 3.3 mm diameter/ 48 with 4.1 mm diameter) were loaded after 3 months of submerged healing. The restoration consisted of an individually designed full‐zirconia abutment veneered with porcelain. Follow‐up with clinical and radiographic assessment was conducted until 60 months after the final restoration. Aesthetic outcome of the restoration was determined with the Pink Esthetic Score–White Esthetic Score (PES‐WES). Patient satisfaction was assessed with a VAS scale and satisfaction questionnaire.ResultsFifty patients completed the 5‐year follow‐up. Implant survival was 100%, restoration survival 98%. Mean bone‐level change was −0.13 ± 0.66 mm with a median (IQR) pocket probing depth of 2.75 [2.25; 3.25]. The mean PES and WES scores were 6.6 ± 1.7 and 7.8 ± 1.5, respectively. Patient satisfaction was high (92.1 ± 7.8 on 100 mm VAS scale). There were no differences between patients with or without a bone augmentation procedure.ConclusionBone‐level implants with a conical connection are a reliable treatment option in single‐tooth replacements in the maxillary aesthetic zone.
Prognosis of oral squamous cell carcinoma with perineural invasion: A comparative study of classification types
Authors: Park Jae , Megow Anna , Swalling Adam , Hodge John‐Charles , Foreman Andrew , Boase Samuel , Valentine Rowan , Krishnan Suren , Ooi Eng H. 
Published: November 2019

AbstractObjectiveTo investigate the histological location and extent of perineural invasion (PNI) as prognostic factors.DesignRetrospective review of medical records and histological analysis of 116 patients with oral squamous cell carcinoma (OSCC).SettingTwo major public tertiary hospitals treating head and neck cancer, Royal Adelaide Hospital and Flinders Medical Centre, in South Australia.ParticipantsPatients diagnosed with OSCC who underwent primary surgical treatment with curative intent at these two centres from January 1, 2005 through December 31, 2015.Main Outcome MeasuresThe primary end points were disease‐free survival (DFS) and disease‐specific survival (DSS).ResultsThe presence of PNI as a binary factor alone did not significantly influence the clinical outcomes. Extratumoural (ET) PNI as measured from the tumour edge was associated with worse DFS on multivariate analyses. Multifocal PNI was associated with worse DFS and DSS. DFS in multifocal PNI was worse irrespective of whether adjuvant therapy was administered.ConclusionsThe presence of multifocal and ET PNI in OSCC is associated with poor clinical outcomes. Patients with multifocal PNI were associated with worse DFS even with adjuvant therapy.
Matrix metalloproteinases MMP‐1, MMP‐2, and MMP‐13 are overexpressed in primary nodular melanoma
Authors: Zamolo Gordana , Grahovac Maja , Žauhar Gordana , Vučinić Damir , Kovač Leo , Brajenić Nika , Grahovac Blaženka 
Published: November 2019

AbstractBackgroundThe spread and invasion of malignant melanoma cells involve degradation and reorganization of the extracellular matrix by the activation of several matrix metalloproteinases (MMPs). This study analyzed the expression of MMP‐1, MMP‐2, and MMP‐13 proteins in primary nodular melanoma (NM) and dysplastic nevi (DN) as a significant risk factor for melanoma development. The secondary goal was to analyze the correlation of MMPs protein expression in NM with tumor invasion, BRAF V600 mutation status, and overall survival.MethodsImmunohistochemistry for MMP‐1, MMP‐2, and MMP‐13 was performed on nodular melanoma (n = 52) and dysplastic nevi (n = 28) on tissue microarray (TMA). BRAF V600 mutation analysis on NM samples was performed by the Sanger sequencing method.ResultsA high level of MMPs expression in NM samples (>30%) compared with DN (<8%) was statistically significant (P < 0.001). BRAF V600 mutations were detected in 15 of 39 (38.5%) NM samples. This study revealed an interesting finding that MMP‐1 and MMP‐13 protein expression in the BRAF V600 mutated melanomas were significantly lower than in the BRAF V600 wild type (P < 0.05).ConclusionCox analysis revealed that Clark categories, Breslow thickness, and MMP‐1 high protein expression are predictive factors for shorter overall survival (P < 0.05).
Intercropping impacts the host location behaviour and population growth of aphids
Authors: Mansion‐Vaquié Agathe , Ferrer Aurélie , Ramon‐Portugal Felipe , Wezel Alexander , Magro Alexandra 
Published: November 2019

AbstractIncreasing intrafield plant diversity has been shown to regulate pest populations in various agroecosystems. Among the suggested mechanisms for this bottom‐up pest control, the disruptive crop hypothesis states that herbivores' abilities to locate and colonize their host plants are reduced by the presence of non‐host plants. Under laboratory conditions, we evaluated how intercropping wheat and legumes modifies the behaviour of apterous cereal aphids,Sitobion avenae(Fabricius) (Hemiptera: Aphididae), in terms of host plant location and population growth. We compared two intercropping systems – soft winter wheat,Triticum aestivumL. (Poaceae), associated with winter pea,Pisum sativumL., or with white clover,Trifolium repensL. (both Fabaceae) – and sole stands of soft winter wheat. Aphids needed more time to locate their wheat host plant and then spent less time on wheat when it was intercropped with clover. At the population level, and accounting for host plant biomass, only intercropping wheat with clover significantly reduced aphid densities on wheat, as this was particularly disruptive toS. avenaebehaviour and population growth.Our laboratory study points out that the species used as non‐host plants and their density are important parameters that should be taken into account in field studies on intercropping systems.
Intercropping oilseed rape as a potential relay crop for enhancing the biological control of green peach aphids and aphid‐transmitted virus diseases
Authors: Lai Rongquan , Hu Hanqing , Wu Xiaoting , Bai Jingjing , Gu Gang , Bai Jianbao , Zhou Ting , Lin Tianran , Zhong Xiujin 
Published: November 2019

AbstractTobacco viruses transmitted by green peach aphids, Myzus persicae (Sulzer) (Hemiptera: Aphididae), cause severe disease in flue‐cured tobacco, Nicotiana tabacum L. (Solanaceae), in China and throughout the world. Field experiments were conducted in 2016 and 2017 in Longyan City, Fujian Province, China, to determine whether M. persicae and aphid‐transmitted virus diseases are affected by intercropping of oilseed rape, Brassica napus L. (Brassicaceae), in tobacco fields. The results showed that, compared with those in monocultured fields, the densities of M. persicae and winged aphids in intercropped fields significantly decreased in both 2016 and 2017. In particular, the appearance of winged aphids was delayed by ca. 7 days. Moreover, the densities of Aphidius gifuensis Ashmead (Hymenoptera: Aphidiidae), a parasitoid of the aphid, significantly increased in 2016 and 2017. Accordingly, the incidence rates of aphid‐transmitted virus diseases (those caused by the cucumber mosaic virus, potato virus Y, and tobacco etch virus) significantly decreased in the intercropped fields in 2016 and 2017. Tobacco yields and monetary value significantly increased in 2016 (by 10–25 and 14–29%, respectively) and 2017 (by 17–22 and 22–34%, respectively). Consequently, our results suggest that intercropping oilseed rape in tobacco fields is a good approach to regulating and controlling aphids and tobacco mosaic viruses, for example potyvirus, and this intercropping can help control aphid‐transmitted virus diseases in tobacco.
Perceptions of interprofessional collaboration in education of dentists and dental hygienists and the impact on dental practice in the Netherlands: A qualitative study
Authors: Kersbergen Maria J. , Creugers Nico H. J. , Hollaar Vanessa R. Y. , Laurant Miranda G. H. 
Published: November 2019

AbstractIntroductionThe purpose of this study was to evaluate the perceptions of dentists and dental hygienists regarding their own and each other's roles in interprofessional collaboration following a clinical interprofessional educational programme (CIEP) as part of their graduate programme at Radboud university medical center (Radboudumc) and HAN University of Applied Sciences (HAN UAS), Nijmegen, the Netherlands, respectively. Perceptions were obtained at the end of their study (2014) and two years after their graduation (2016).MethodsA qualitative study was conducted using a questionnaire with open‐ended questions. Sixty‐two dentists and thirty‐eight dental hygienists were invited. Theory‐based analyses were applied in combination with principles of Grounded Theory to analyse and synthesise the collected data from the open‐ended questions.ResultsAt baseline, 100% responded. At follow‐up, thirty‐two dentists (51.6%) and twenty‐six dental hygienists (68.4%) responded; twenty‐seven dentists (43.5%) and eighteen dental hygienists (47.4%) were included. Many similarities were found between baseline and follow‐up regarding perceptions of each other's expertise and responsibility, learning from and with each other, and the behaviour of students and the “novice” professionals in interprofessional collaboration. Both dentists and dental hygienists experienced the CIEP as useful for interprofessional collaboration. The “novice” dentists and dental hygienists indicated that in dental practices interprofessional collaboration was less common.ConclusionThe CIEP resulted in more understanding amongst dentists and dental hygienists with regard to interprofessional roles, but in practice the “novice” dentists and dental hygienists face difficulties in applying the interprofessional roles.
Long‐lasting modifications of motoneuron firing properties by trans‐spinal direct current stimulation in rats
Authors: Bączyk Marcin , Drzymała‐Celichowska Hanna , Mrówczyński Włodzimierz , Krutki Piotr 
Published: November 2019

AbstractTrans‐spinal direct current stimulation (tsDCS) is a novel neuromodulatory technique that has been used during neurological rehabilitation and sports to modulate muscle activation. However, the physiological mechanisms that underly the long‐lasting functional effects of polarization are not yet fully understood, nor are their relationships with specific neuronal populations. The acute facilitatory and depressive effects of anodal and cathodal polarization on motoneurons have been recently demonstrated, and the aim of this study was to determine whether tsDCS‐evoked modulations of motoneuron properties are able to persist over several hours. Intracellular recordings from multiple antidromically identified rat motoneurons were performed both before and after the application of tsDCS (0.1 mA for 15 min), at various time points up to 180 min after the offset of anodal or cathodal tsDCS. The examined effects of anodal polarization included decreased rheobase, voltage threshold, the minimum and maximum currents necessary for rhythmic firing, increased rhythmic firing frequencies and the slope of the f–I relationship. The majority of these facilitatory changes to threshold and firing properties were sustained for 30–60 min after polarization. In contrast, the significant effects of cathodal polarization were absent, except the short‐lasting decreased ability for motoneurons to induce rhythmic activity. This study provides direct evidence that a single polarization session can alter the electrophysiological properties of motoneurons for at least one hour and provides a basis for the further use of tsDCS techniques under conditions where the sustained modification of motoneuron firing is desired.
Phase selective crystallization of Na2Mn0.9Fe0.1P2O7 glass by laser irradiation
Authors: Honma Tsuyoshi , Kumagai Yu , Komatsu Takayuki 
Published: November 2019

AbstractLaser‐induced crystallization was performed to form zero‐dimensional (dot) and one‐dimensional (line) patterns on the surface of Na2Mn0.9Fe0.1P2O7 glass. X‐ray diffraction patterns of crystallized‐glass prepared by conventional heat treatment suggests that the formation of β‐Na2MnxFe1−xP2O7 phase is more likely to form than layered‐ (L‐) Na2MnxFe1−xP2O7, which is the other metastable polymorph. Using the continuous wave (CW) laser‐induced crystallization technique, a demonstration of phase selective crystal patterning was confirmed in Na2Mn0.9Fe0.1P2O7 glass. Depending on the laser power (P), micro‐Raman spectroscopy of dot pattern suggest that three types of structural modifications, that is, softening, crystallization of L‐phase structure polymorph, β‐phase, and a mixture of L‐ and β‐phase, were induced. Meanwhile, the line patterning of Na2Mn0.9Fe0.1P2O7 showed a slow growth rate of 2 µm s−1. At P = 0.73 W, even when scanning was started from the β‐dot, the growth of the β‐line was disturbed by the formation of L‐phase but stable line growth of β‐phase occur at P = 0.76 W. Although the formation rate was also slow, laser irradiation is possible to form metastable polymorph selectively.
Molar‐incisor malformation: Eight new cases and a review of the literature
Authors: Vargo Richard J. , Reddy Rekha , Da Costa Walmir B. , Mugayar Leda R.F. , Islam Mohammed N. , Potluri Anitha 
Published: November 2019

AbstractBackgroundMolar‐incisor malformation (MIM) is a recently described dental abnormality. While MIM mimics dentin dysplasia, it presents in a localized pattern. Furthermore, it is speculated that MIM is caused by significant early‐life medical history.AimThe purpose of this study is to present a series of MIM cases and compare the findings with the literature.DesignAn extensive search of all published cases of MIM in the English‐language literature was conducted. Additionally, an institutional review board‐approved retrospective search was performed within the University of Florida oral pathology biopsy service archives. Radiographic consultation cases were also included. Cases lacking radiographs were excluded.ResultsSeventy‐nine cases were identified in the literature, and eight cases were identified in our retrospective search. All but one case involved the permanent first molars. The average age at diagnosis was 9 years. Many patients reported significant early‐life medical histories.ConclusionsMIM usually affects the permanent first molars and may be linked to early‐life medical conditions or interventions. Oral healthcare providers, especially paediatric dentists, should be aware of MIM to avoid misdiagnosing it as dentin dysplasia. Long‐term follow‐up studies with thorough medical history documentation are essential to understand the pathogenesis and aetiology and to create treatment guidelines.
Clinical characteristics of insulin resistance syndromes: A nationwide survey in Japan
Authors: Takeuchi Takehito , Ishigaki Yasushi , Hirota Yushi , Hasegawa Yutaka , Yorifuji Tohru , Kadowaki Hiroko , Akamizu Takashi , Ogawa Wataru , Katagiri Hideki 
Published: November 2019

AbstractAims/IntroductionInsulin resistance syndrome (IRS) of type A or B is triggered by gene abnormalities of or autoantibodies to the insulin receptor, respectively. Rabson–Mendenhall/Donohue syndrome is also caused by defects of the insulin receptor gene (INSR), but is more serious than type A IRS. Here, we carried out a nationwide survey of these syndromes in Japan.Materials and MethodsWe sent questionnaires to a total of 1,957 academic councilors or responsible individuals at certified facilities of the Japan Diabetes Society, as well as at the department pediatrics or neonatology in medical centers with >300 beds.ResultsWe received 904 responses with information on 23, 30 and 10 cases of type A or B IRS and Rabson–Mendenhall/Donohue syndrome, respectively. Eight cases with type A IRS‐like clinical features, but without an abnormality of INSR, were tentatively designated type X IRS, with five of these cases testing positive for PIK3R1 mutations. Fasting serum insulin levels at diagnosis (mean ± standard deviation) were 132.0 ± 112.4, 1122.1 ± 3292.5, 2895.5 ± 3181.5 and 145.0 ± 141.4 μU/mL for type A IRS, type B IRS, Rabson–Mendenhall/Donohue syndrome and type X IRS, respectively. Type A and type X IRS, as well as Rabson–Mendenhall/Donohue syndrome were associated with low birthweight. Type B IRS was diagnosed most frequently in older individuals, and was often associated with concurrent autoimmune conditions and hypoglycemia.ConclusionsInformation yielded by this first nationwide survey should provide epidemiological insight into these rare conditions and inform better healthcare for affected patients.