bluuebunny/crossref_metadata_2025_split huggingface dataset:
An Electrochemically Synthesized Nanoporous Copper Microsensor for Highly Sensitive and Selective Determination of Glyphosate
Authors: Regiart Matias , Kumar Abhishek , Gonçalves Josué M. , Silva Junior Gilberto J. , Masini Jorge César , Angnes Lúcio , Bertotti Mauro 
Published: February 2020

AbstractA nanoporous copper (NPC) film was electrodeposited on a copper microelectrode, and the generated platform was investigated for electrochemical sensing of glyphosate (Glyp). The as‐deposited NPC film was highly pure and crystalline according to results of energy dispersive spectroscopy and X‐ray diffraction experiments, respectively. Scanning electron microscopy images confirm the NPC films possess a highly porous morphology containing dendrite fractals, and the electrodeposition parameters, particularly potential (Ed) and time (td), exert a remarkable influence on the structure of the films. Such changes in the NPC morphology with Ed and td were also correlated with the electrochemical behavior investigated by cyclic voltammetry. In the presence of Glyp, the anodic oxidation is facilitated because copper ions diffuse easily through the pores of the NPC film and form a complex with the analyte at the electrode interface. On the other hand, as the amount of copper oxides decreases due to the formation of soluble Cu(II) complex with Glyp, less current is obtained during the reverse scan, allowing a relationship between the decrease in the cathodic current and the Glyp concentration to be established. The optimized NPC‐modified Cu microelectrode showed very high sensitivity (14 nA nmol−1 L), low detection limit (4 nmol L−1), excellent reproducibility, and selective response for Glyp. The applicability of the sensor was demonstrated by detecting Glyp in river water samples.
Strategies for the Controlled Covalent Double Functionalization of Graphene Oxide
Authors: Vacchi Isabella A. , Guo Shi , Raya Jésus , Bianco Alberto , Ménard‐Moyon Cécilia 
Published: February 2020

AbstractGraphene oxide (GO) is a versatile platform with unique properties that have found broad applications in the biomedical field. Double functionalization is a key aspect in the design of multifunctional GO with combined imaging, targeting, and therapeutic properties. Compared to noncovalent functionalization, covalent strategies lead to GO conjugates with a higher stability in biological fluids. However, only a few double covalent functionalization approaches have been developed so far. The complexity of GO makes the derivatization of the oxygenated groups difficult to control. The combination of a nucleophilic epoxide ring opening with the derivatization of the hydroxyl groups through esterification or Williamson reaction was investigated. The conditions were selective and mild, thus preserving the structure of GO. Our strategy of double functionalization holds great potential for different applications in which the derivatization of GO with different molecules is needed, especially in the biomedical field.
Phosphorene‐Based Electrocatalysts
Authors: Dinh Khang Ngoc , Zhang Yu , Zhu Jixin , Sun Wenping 
Published: February 2020

AbstractPhosphorene, generally defined as two‐dimensional (2D) black phosphorus (BP) with monolayered or few‐layered structure, has emerged as a promising member of the family of 2D materials. Since its discovery in 2014, extensive research has been focused on broadening its applications, covering the biological, photoelectric, and electrochemical fields, owing to the unique physicochemical and structural properties. As a single‐elemental material, phosphorene has demonstrated its applicability for the preparation of efficient electrocatalysts for hydrogen evolution reaction (HER), oxygen evolution reaction (OER), nitrogen reduction reaction (NRR), and other electrocatalytic applications. In this Minireview, a summary of the very recent research progresses of phosphorene in electrocatalysis is offered, with a special focus on the effective synthetic strategies towards performance improvement. In the concluding section, challenges and perspectives are also discussed.
Organizational buying behaviour for perishable‐food packaging in grocery retail
Authors: Afif Karima , Rebolledo Claudia , Roy Jacques 
Published: February 2020

AbstractThis qualitative study investigates the structure of the buying centre, the buying process, and the factors that influence buying decisions for perishable‐products packaging. This empirical investigation is based on the case study of a major Quebec food retailer. We show that the purchase of packaging changes significantly depending on whether the product is considered more or less strategic for the organization, which determines the required level of packaging customization. Buying centre structure becomes more complex as packaging customization increases. Given the multi‐functionality and the interdisciplinarity of packaging, we propose a multi‐criteria analysis grid to improve buying decision efficiency and to streamline communication between the various levels of the buying centre.
Extracapsular extension of neck nodes and absence of human papillomavirus 16‐DNA are predictors of impaired survival in p16‐positive oropharyngeal squamous cell carcinoma
Authors: Freitag Josefine , Wald Theresa , Kuhnt Thomas , Gradistanac Tanja , Kolb Marlen , Dietz Andreas , Wiegand Susanne , Wichmann Gunnar 
Published: February 2020

BackgroundHuman papillomavirus (HPV)‐driven oropharyngeal squamous cell carcinomas (OPSCCs) demonstrate superior outcome compared with HPV‐negative OPSCCs. The eighth edition of the American Joint Committee on Cancer/Union for International Cancer Control (AJCC/UICC) tumor, lymph node, metastasis (TNM) classification (TNM 2017) modifies OPSCC staging based on p16 positivity as a surrogate for HPV‐driven disease. In p16‐negative OPSCCs, lymph node (N) categories include extracapsular/extranodal extension (ECE); and, in p16‐positive OPSCCs, N categories are based on the number of positive neck lymph nodes omitting ECE status. The objective of the current study was to assess the prognostic impact of positive ECE status and the detection of HPV16 DNA in patients with p16‐positive OPSCC.MethodsIn a cohort of 92 patients with p16‐positive, lymph node (N)‐positive (stage III‐IVB) OPSCC who underwent surgery and neck dissection, allowing for a pathologic examination of positive lymph nodes, 66 of 92 patients (71.4%) were p16‐positive/HPV16 DNA‐positive, 62 of 92 (67%) were ECE‐positive, and 45 of 62 (72.6%) were ECE‐positive, p16‐positive, and HPV16 DNA‐positive. Differences in outcome were assessed using Kaplan‐Meier plots and Cox proportional hazard regression (CoxR) for tumor‐specific survival and overall survival (OS).ResultsThe mean numbers of positive lymph nodes in ECE‐positive patients (5.0 positive lymph nodes; 95% CI, 3.8‐6.4 positive lymph nodes) and ECE‐negative patients (2.4 positive lymph nodes; 95% CI, 1.8‐2.9 positive lymph nodes) were different (P = .0007). ECE affected OS and tumor‐specific survival in p16‐positive patients (P = .007 and P = .047, respectively) and in p16‐positive/HPV16 DNA‐positive patients (P = .013 and P = .026, respectively). Related to the unequal distributions of ECE‐positive/HPV16 DNA‐negative tumors, the TNM 2017 failed to discriminate OS in patients with UICC stage I, II, and III disease (mean OS, 54.5, 73.4, and 45 months, respectively; median OS, 64.7 months, not reached, and 41.1 months, respectively). According to a univariate CoxR, the presence of ECE predicted impaired OS in patients with p16‐positive OPSCC (hazard ratio, 3.40; 95% CI, 1.17‐9.89; P = .025) and even greater impaired OS in those with p16‐positive/HPV16 DNA‐positive OPSCC (HR, 8.64; 95% CI, 1.12‐66.40; P = .038). Multivariate CoxR confirmed ECE and HPV16 DNA detection as independent predictors.ConclusionsECE and HPV16 DNA status should be included in the prognostic staging of patients with p16‐positive OPSCC because several lines of evidence demonstrate their impact on survival.
A Dual Plasmonic Photoelectrode System for Visible‐Light Photocatalysis
Authors: Nishi Hiroyasu , Miyake Koji , Kao Kun‐Che , Tatsuma Tetsu 
Published: February 2020

AbstractPlasmonic photocathodes based on plasmon‐induced charge separation (PICS) were prepared by depositing an Au nanoparticle (AuNP) ensemble on a transparent electrode and coating it with compact TiO2. Its PICS efficiency was improved by coating it further with porous TiO2 or introducing a NiO blocking layer as an underlayer of the AuNP ensemble. The former photocathode was modified further with Pt co‐catalyst nanoparticles, and combined with a PICS photoanode fabricated by depositing a AuNP ensemble on compact TiO2 and coating it with a Ni(OH)2 charge accumulation layer. The dual plasmonic photoelectrode system thus obtained exhibited a higher efficiency than the sum of the efficiencies of the single photoanode and single photocathode.
Effectiveness of motor imagery and action observation training on musculoskeletal pain intensity: A systematic review and meta‐analysis
Authors: Suso‐Martí Luis , La Touche Roy , Angulo‐Díaz‐Parreño Santiago , Cuenca‐Martínez Ferran 
Published: February 2020

AbstractBackground and ObjectiveMovement representation techniques such as motor imagery (MI) and action observation (AO) could play an important role in the field of rehabilitation of patients with musculoskeletal pain; however, the effects of these tools on clinical pain remain unclear. Our objective is therefore to develop a systematic review and meta‐analysis of the effects of MI and AO regarding the pain intensity on patients with musculoskeletal pain.Databases and Data TreatmentMEDLINE, EMBASE, CINAHL and Google Scholar were searched. Last search was run on July 2019. Meta‐analysis was conducted to determine the effectiveness on pain intensity in patients with post‐surgical pain or chronic pain, and GRADE was used to rate the quality, certainty and applicability of the evidence.ResultsA total of 10 studies were included. AO or MI were compared versus usual rehabilitation care. The meta‐analysis showed statistically significant differences in MI and AO interventions in patients with pain after surgery in six studies, with a large clinical effect (n = 124; SMD = −0.84; 95% CI: −1.47 to −0.21). In patients with chronic pain, results showed statistically significant differences in MI and AO interventions in three studies, with a large clinical effect (n = 83; SMD = −2.14; 95% CI: −3.81 to −0.47). Risk of bias, imprecision and inconsistency decreased the GRADE level of evidence.ConclusionsThere was very low‐quality evidence regarding the effects of movement representation techniques intervention (AO and MI) on pain intensity in patients with post‐surgical pain. In addition, there was very low‐quality evidence regarding the effects of MI and AO interventions on chronic pain.SignificanceMovement representation techniques in combination with usual care are capable of producing a decrease in pain intensity compared with conventional treatment, in both post‐surgical and chronic pain. However, the very low‐quality evidence found regarding these techniques showed that more research is needed for their application in a clinical context.
Photoelectrochemical Detection of L‐Cysteine with a Covalently Grafted ZnTAPc‐Gr‐based Probe
Authors: Peng Jinyun , Huang Qing , Liu Yuxia , Huang Yingying , Zhang Cuizhong , Xiang Gang 
Published: February 2020

AbstractL‐cysteine plays a vital role in organisms, and is an important biomarker for many pathological diseases that seriously affect human health. In the study, a photoelectrochemical (PEC) probe with zinc‐tetramine phthalocyanine covalently grafted to graphene oxide (ZnTAPc‐Gr) was developed for L‐cysteine detection. Graphene oxide (GO) with carboxyl was used to immobilize zinc‐tetramine phthalocyanine (ZnTAPc) with amidogen (a graft structure formed by an amide covalent bond), which could firmly immobilize ZnTAPc, thereby improving the photoelectrochemical performance and stability. L‐cysteine molecule, an electron acceptor, was specifically recognized by the PEC probe, exhibiting a decrease in the photocurrent signal. Under the optimal experimental conditions, the fabricated PEC probe exhibited excellent performance in L‐cysteine analysis within a linear range of 0.25–113 μM and a detection limit of 11.4 nM. The PEC probe showed high sensitivity, selectivity, and stability. The method described herein provides an effective strategy for PEC probe construction for L‐cysteine detection, and can also serve as a promising PEC platform for the analyses of other small molecules.
A free amino acid‐based diet partially prevents symptoms of cow's milk allergy in mice after oral sensitization with whey
Authors: van Sadelhoff Joris H. J. , Hogenkamp Astrid , Wiertsema Selma P. , Harthoorn Lucien F. , Loonstra Reinilde , Hartog Anita , Garssen Johan 
Published: February 2020

AbstractBackgroundAmino acid‐based formulas (AAFs) are used for the dietary management of cow's milk allergy (CMA). Whether AAFs have the potential to prevent the development and/or symptoms of CMA is not known.ObjectiveThe present study evaluated the preventive effects of an amino acid (AA)‐based diet on allergic sensitization and symptoms of CMA in mice and aimed to provide insight into the underlying mechanism.MethodsC3H/HeOuJ mice were sensitized with whey protein or with phosphate‐buffered saline as sham‐sensitized control. Starting 2 weeks before sensitization, mice were fed with either a protein‐based diet or an AA‐based diet with an AA composition based on that of the AAF Neocate, a commercially available AAF prescribed for the dietary management of CMA. Upon challenge, allergic symptoms, mast cell degranulation, whey‐specific immunoglobulin levels, and FoxP3+ cell counts in jejunum sections were assessed.ResultsCompared to mice fed with the protein‐based diet, AA‐fed mice had significantly lower acute allergic skin responses. Moreover, the AA‐based diet prevented the whey‐induced symptoms of anaphylaxis and drop in body temperature. Whereas the AA‐based diet had no effect on the levels of serum IgE and mucosal mast cell protease‐1 (mMCP‐1), AA‐fed mice had significantly lower serum IgG2a levels and tended to have lower IgG1 levels (P = .076). In addition, the AA‐based diet prevented the whey‐induced decrease in FoxP3+ cells. In sham‐sensitized mice, no differences between the two diets were observed in any of the tested parameters.ConclusionThis study demonstrates that an AA‐based diet can at least partially prevent allergic symptoms of CMA in mice. Differences in FoxP3+ cell counts and serum levels of IgG2a and IgG1 may suggest enhanced anti‐inflammatory and tolerizing capacities in AA‐fed mice. This, combined with the absence of effects in sham‐sensitized mice indicates that AAFs for the prevention of food allergies may be an interesting concept that warrants further research.
Role of the interferons in CD64 and CD169 expressions in whole blood: Relevance in the balance between viral‐ or bacterial‐oriented immune responses
Authors: Bourgoin Pénélope , Biéchelé Géraldine , Ait Belkacem Inès , Morange Pierre‐Emmanuel , Malergue Fabrice 
Published: February 2020

AbstractIntroductionCD64 expression increases on neutrophils during bacterial infections. Recently an increase in CD169 expression has been discovered on monocytes during viral infections. Generally, interferons α (IFNsα) and IFNsγ are key drivers of the infectious host immune response. The purpose of this study was to explore if a link exists between these IFNs and both biomarkers.MethodsWhole blood samples from healthy volunteers were stimulated with either IFNs, interleukins, or infectious extracts, to mimic an infectious state. Expressions of CD64 and CD169 were assessed in these samples by multiple flow cytometry methods, over precise kinetics.ResultsThe expression of CD64 was statistically higher in samples stimulated with IFNγ, and CD169 in those stimulated with IFNα (and all other type I IFNs). Surface expressions are directly induced by their respective IFNs via Janus kinase/signal transducer and activator of transduction pathways within 6 to 8 hours of incubation. Mixing both types of IFNs seemed to indicate that they partially inhibit each other.ConclusionsThe induction of CD169 on monocytes and CD164 on neutrophils by type I and type II IFNs confirms the relevance of these markers for assessing between a viral‐ vs bacterial‐oriented immune response.
Derivation of cancer no significant risk levels and screening safety assessment for 2‐nitropropane in spray products
Authors: Gadagbui Bernard , Moore Justin , Parker Ann , McCready David , Monnot Andrew D. , Garnick Lindsey , Vincent Melissa , Spencer Pamela , Maier Andrew 
Published: February 2020

AbstractTwo proposition 65 no‐significant‐risk level (NSRL)‐type values were derived for 2‐nitropropane (2‐NP), in the absence of a Californian published NSRL. In addition, a safety assessment was performed based on estimated typical consumer inhalation and dermal exposure to 2‐NP during indoor application of paint from a spray can containing the solvent 1‐nitropropane. For the NSRL derivation, benchmark dose (BMD) modeling was performed using hepatocellular carcinoma incidence data from 2‐NP single exposure inhalation studies in Sprague‐Dawley rats. Several BMD models provided an acceptable fit for the male rat hepatocellular carcinoma incidence data (gamma, log‐probit, log‐logistic and multistage); therefore, the mean of the BMD lower limits from each model were used as the point of departure to derive the inhalation cancer potency. The oral human cancer potency was derived from the inhalation human cancer potency based on the ratio of the uptake factors for inhalation vs. oral routes. The derived inhalation and oral NSRLs are 67 μg/day and 32 μg/day, respectively. For the inhalation and dermal exposure assessment, three key factors were analyzed: the 2‐NP residual concentration in the spray paint product, the mass of spray paint used and the frequency of use. Based on the screening exposure assessment, potential consumer inhalation and dermal exposure to 2‐NP from indoor application of paint from a spray can does not exceed our proposed NSRLs, and a warning label is therefore not required for spray can products containing the solvent 1‐nitropropane where 2‐NP is a minor contaminant.
MicroRNA‐1 facilitates hypoxia‐induced injury by targeting NOTCH3
Authors: Xu Jinjin , Cao Dandan , Zhang Daping , Zhang Yuan , Yue Yuxia 
Published: February 2020

AbstractCell proliferation, apoptosis, and autophagy have been reported to be related to myocardial ischemia injury. MicroRNAs have attracted wide attention on regulating cell proliferation, apoptosis, and autophagy. miR‐1 expression has been reported to be dysregulated in cardiac tissue or cells with hypoxia, while the exact roles as well as underlying mechanism remain poorly understood. In this study, we investigated the potential roles of miR‐1 in cell proliferation, apoptosis, and autophagy in hypoxia‐treated cardiac injury and explored the underlying mechanism using H9c2 cells. Results showed that hypoxic stimulation inhibited cell proliferation and the expression of miR‐1 but promoted cell apoptosis in H9c2 cells. Moreover, overexpression of miR‐1 promoted cell apoptosis and inhibited cell proliferation and autophagy in H9c2 cells treated with hypoxia, while its knockdown played an opposite effect. In addition, bioinformatics, luciferase reporter, and RNA immunoprecipitation analyses indicated that NOTCH3 was a direct target of miR‐1 and its upregulation reversed the effects of miR‐1 on cell proliferation, apoptosis, and autophagy in hypoxia‐treated H9c2 cells. Taken together, our data suggested that miR‐1 promoted hypoxia‐induced injury by targeting NOTCH3, indicating novel therapeutic targets for treatment of myocardial ischemia injury.
NCALD affects drug resistance and prognosis by acting as a ceRNA of CX3CL1 in ovarian cancer
Authors: Dong Caihua , Yin Fuqiang , Zhu Dan , Cai Xiangxue , Chen Cuilan , Liu Xia 
Published: February 2020

AbstractDrug resistance, an impenetrable barrier in the treatment of ovarian cancer (OC), is often associated with poor outcomes. Hence, it is urgent to discover new factors controlling drug resistance and survival. The association between neurocalcin delta (NCALD) and cancer drug resistance is poorly understood. Here, we reveal that NCALD messenger RNA expression, probably regulated by DNA methylation and microRNAs, was significantly downregulated in at least three independent microarrays covering 633 ovarian carcinomas and 16 normal controls, which includes the Cancer Genome Atlas (TCGA) ovarian cohort. In the sub‐groups of the TCGA cohort, NCALD was suppressed in 90 platinum‐resistant tissues vs in 197 sensitive tissues. It is consistent with the quantitative reverse transcription polymerase chain reaction results revealing gene downregulation in carboplatin‐resistant SKOV3 and HeyA8 OC cells as compared with that in controls. Low expression of NCALD predicted poor overall survival (OS) in sub‐groups of 1656 patients, progression‐free survival (PFS) in 1435 patients, and post‐progression survival (PPS) in 782 patients according to Kaplan‐Meier plotter covering 1815 OC patients. Comprehensive bioinformatic analyses strongly implicated NCALD in the regulation of drug resistance, probably via competing for endogenous RNA (ceRNA) interactions with CX3CL1 and tumor immune‐microenvironment. NCALD acted as a ceRNA for CX3CL1 in 21 different cancers includes OC according to Starbase. These two genes negatively correlated with tumor purity and positively correlated with infiltration levels of neutrophils and dendritic cells in OC. The combined low expression of NCALD and CX3CL1 showed better prognosis potential for OS, PFS, and PPS in the 1815 OC patients than any of the individually tested genes. In summary, NCALD acts as a ceRNA for CX3CL1, and its downregulation may affect drug resistance and prognosis in OC. Thus, NCALD could be a new therapeutic target for anticancer therapy and a new biomarker for survival prediction in OC.
Identification immunophenotyping of lung adenocarcinomas based on the tumor microenvironment
Authors: Tan Qilong , Huang Yue , Deng Kui , Lu Mingliang , Wang Liuying , Rong Zhiwei , Zhao Weiwei , Li Shuang , Xu Zhenyi , Fan Lijun , Li Kang , Li Zhenzi 
Published: February 2020

AbstractThe tumor immune microenvironment is heterogeneous, and its impact on treatment responses is not well understood. It is still a challenge to analyze the interaction between malignant cells and the tumor microenvironment to apply suitable immunotherapy in lung adenocarcinoma. We performed the nonnegative matrix factorization method to 513 messenger RNA expression profiles of lung adenocarcinomas (LUADs) from The Cancer Genome Atlas (TCGA) to obtain an immune‐related expression pattern. Subsequently, we characterized the immune‐related gene signatures and clinical and survival characteristics. We used 576 patients from Gene Expression Omnibus to confirm our findings. Of the patients in the training cohort, 51% had a high immune enrichment score, high expression of immune cell signaling, cytolytic activity, and interferon (IFN)‐related signatures (all P < .05). We denoted these as the Immune Class. We further subdivided the Immune Class into two subclasses based on the tumor microenvironment. These were denoted the Active Immune Class and Exhausted Immune Class. The former showed significant IFN, T‐cells, M1 macrophage signatures, and better prognosis (all P < .05), while the latter presented an exhausted immune response with activated stromal enrichment, M2 macrophage signatures, and immunosuppressive factors such as WNT/transforming growth factor‐β (all P < .05). Furthermore, we predicted the response of our immunophenotypes to immunological checkpoint inhibitors (P < .05). Our findings provide a novel insight into the immune‐related state of LUAD and can identify the patients who will be receptive to suitable immunotherapeutic treatments.