bluuebunny/crossref_metadata_2025_split huggingface dataset:
Quantitative Proteomic Study of Peripheral Blood Monocytes Identified Novel Genes Involved in Osteoporosis
Authors: Xu Qing , Xu Li , He Pei , Sun Yang-Hua , Lu Xin , Lei Shu-Feng , Deng Fei-Yan 
Published: November 2020

Objective:
Osteoporosis (OP) is mainly characterized by low Bone Mineral Density (BMD)
and microarchitectural deterioration of bone tissue. We performed label-free quantitative proteomics
to discover novel proteins involved in the pathogenesis of osteoporosis.

Methods:
We employed an extreme sampling study design to collect subjects with low BMD (Z-score<-
1.30±0.47) and high BMD (Z-score>1.06±0.49). Liquid Chromatography and Mass Spectrometry
(LC-MS) technologies were used to identify Peripheral Blood Monocyte (PBM)-expressed
proteins significant for OP in Chinese elderly women (Study Sample 1) and men (Study
Sample 2), respectively.

Results:
A total of 131 Differentially Expressed Proteins (DEPs) and 200 DEPs were identified in
subjects with low vs. high BMD from the study samples 1 and 2, respectively. Interestingly, three
DEPs (WNK1, SHTN1, and DPM1) were significantly and consistently regulated with BMD in
both genders. GO analysis showed that these DEPs were significantly enriched in “extracellular exosome”,
“protein binding” and “cell-cell adherens junction” (p<0.05). Pathway enrichment results
showed that these DEPs were significantly enriched in “protein ubiquitination”, “ER-Phagosome
pathway” and “antigen processing” (p<0.05). Protein-Protein Interaction (PPI) networks were constructed,
pointing out key node proteins, including HSPA8, PKM, AKT1, and ABI1. Mining data
from independent -omics studies highlighted that 174 DEPs, as identified from above, were significant
for OP in Caucasians as well, including WNK1 and DPM1.

Conclusion:
The study identified known and novel proteins significant for OP in both genders
and/or across ethnicities in both Chinese and Caucasians. Our findings may provide clues for further
research on the underlying pathogenic mechanism of OP.
Iatrogenic Right Atrial Thrombus Complicated by Pulmonary Embolism: Management and Outcomes
Authors: Battisha Ayman , Madoukh Bader , Sawalha Khalid , Patel Brijesh 
Published: November 2020

Abstract:
Right atrial thrombus can originate from distal venous sources or can be iatrogenic, secondary
to the placement of central venous catheters, atrial devices, or surgeries. One of the most
common complications of Central Venous Catheters (CVCs) is thromboembolism, which can be either
fixed to the right atrium or can be free-floating. Device-related Right Atrial Thrombosis
(RAT) can result in catheter occlusion, vascular occlusion, infection, and pulmonary embolism.
The true incidence of these complications is unknown because the diagnosis may not be considered
in asymptomatic patients, and it might be missed by Transthoracic Echocardiography (TTE). In
this literature review, we discuss iatrogenic etiologies of RAT that is complicated by pulmonary
embolism. We highlight the importance of maintaining a high index of suspicion of iatrogenic
RAT, possible complications, and its management.
Synthesis, Aromatase Inhibitory, Antiproliferative and Molecular Modeling Studies of Functionally Diverse D-Ring Pregnenolone Pyrazoles
Authors: Banday Abid H. , Saeed Bahjat A. , Al-Masoudi Najim A. 
Published: November 2020

Background:
Aromatase, a cytochrome P450 hemoprotein that is responsible for estrogen
biosynthesis by conversion of androgens into estrogens, has been an attractive target in the treatment of hormonedependent
breast cancer. Design of new steroidal aromatase inhibitors becomes imperative.

Objective:
Synthesis and biological evaluation of two classes of structurally and functionally diverse D-ring pregnenolone pyrazoles as type I aromatase inhibitors and antiproliferative agents.

Methods:
Pregnenolone (1) was converted to 3β-hydroxy-21-hydroxymethylidenepregn-5-en-20-one (2), which
upon cyclization with phenylhydrazine generated regioisomeric pairs of pyrazoles 4 and 5. Further, Knoevenagel
condensation of pregnenolone (1) with 3-oxo-3-phenylpropanenitrile (6) produced 2-benzoyl-3-(3b-hydroxyandrostan-
5-ene-20-ylidene)-but-2-enenitrile (7), which upon cyclization with hydrazine or phenylhydrazine
generated the pyrazoles 8 and 9. All new steroidal derivatives were tested for their aromatase inhibition activity
using Dibenzylfluorescein (DBF) based fluorescence assay developed by Stresser et al. Antiproliferative
activities were measured using Sulforhodamine B assay. The activities were promising and there was a coherence
between aromatase inhibitory and antiproliferative activities.

Results:
The study reveals the immense potential of pregnenolone pyrazoles as aromatase inhibitors for the
treatment of breast cancer. Molecular docking studies proved efficient binding of the new steroidal analogs on
human placental aromatase.

Conclusions:
n the overall study, most of the compounds exhibited potential activity for the treatment of
hormone dependent breast cancer. Compounds 4c and 4d were found to be the most promising pharmacons.
Furthermore, compounds 4c and 4d were applied for their molecular docking study on human placental
aromatase to predict their possible binding modes with the enzyme. These studies revealed that such molecules
have high scope and potential for further investigation towards the treatment of estrogen dependent breast cancer.
Research Status of Subsequent Machining of Laser Cladding Layers
Authors: Li Lei , Cai Yujun , Li Guohe , Liu Meng 
Published: November 2020

Background:
As an important method of remanufacturing, laser cladding can be used to obtain the parts with
specific shapes by stacking materials layer by layer. The formation mechanism of laser cladding determines the “Staircase
effect”, which makes the surface quality can hardly meet the dimensional accuracy of the parts. Therefore, the subsequent
machining must be performed to improve the dimensional accuracy and surface quality of cladding parts.

Methods:
In this paper, chip formation, cutting force, cutting temperature, tool wear, surface quality, and optimization of
cutting parameters in the subsequent cutting of laser cladding layer are analyzed. Scholars have expounded and studied
these five aspects but the cutting mechanism of laser cladding need further research.

Results:
The characteristics of cladding layer are similar to that of difficult to machine materials, and the change of
parameters has a significant impact on the cutting performance.

Conclusion:
The research status of subsequent machining of cladding layers is summarized, mainly from the aspects of
chip formation, cutting force, cutting temperature, tool wear, surface quality, and cutting parameters optimization. Besides,
the existing problems and further developments of subsequent machining of cladding layers are pointed out. The efforts are
helpful to promote the development and application of laser cladding remanufacturing technology.
The Polymorphism of miR-146a (rs2910164) and Breast Cancer Risk: A Meta-Analysis of 17 Studies
Authors: Moossavi Maryam , Shojaee Maryam , Musavi Mahsa , Ibrahimi Reza , Ibrahimi Mostafa , Khorasani Milad 
Published: November 2020

Background:
Single-Nucleotide Polymorphisms (SNPs) in genes responsible for coding
microRNAs (miRNAs) are shown to be crucial in progression of Breast Cancer (BC).

Objective:
The purpose of this meta-analysis is to obtain more definitive and reliable results due to the
ambiguity and inconsistency of the previous findings in this regard. This study aimed at clarifying the
association of mir14a polymorphisms with breast cancer.

Methods:
We searched PubMed, EMBASE, Web of Science and Google Scholar databases for papers
published before August 10, 2019. Afterward, genotypes’ distribution, genotyping methods and ethnicity
groups were extracted and Overall analyses were conducted. A total number of seventeen researches
on 7676 subjects and 7476 controls were found to meet our criteria in this meta-analysis.

Results:
Our observations confirmed the increased risk in breast cancer with rs 2910164 polymorphism
in three genetic models: allele contrast fixed genetic model, Recessive fixed genetic model and
CC vs. GG genetic model (P value 0.0109, 0.0404 and 0.0019, respectively).

Conclusion:
The rs2910164 polymorphism is associated with increased breast cancer risk. We suggest
that more multicenter studies with larger samples investigate this matter to further clarify the association
and verify our findings.
Esaxerenone (CS-3150) in Patients with Type 2 Diabetes and Microalbuminuria (ESAX-DN)
Authors: Ito Sadayoshi , Kashihara Naoki , Shikata Kenichi , Nangaku Masaomi , Wada Takashi , Okuda Yasuyuki , Sawanobori Tomoko 
Published: November 2020

Background and objectives
Diabetic kidney disease is an important complication of type 2 diabetes. In a phase 2b study, adding esaxerenone to renin-angiotensin system inhibitors dose dependently reduced the urinary albumin-to-creatinine ratio in patients with type 2 diabetes and microalbuminuria. This 52-week phase 3 study further investigated the effects of esaxerenone on the urinary albumin-to-creatinine ratio in this patient group.

Design, setting, participants, & measurements
In this multicenter, randomized, double-blind study, patients with type 2 diabetes and a urinary albumin-to-creatinine ratio of 45 to <300 mg/g creatinine treated with renin-angiotensin system inhibitors were randomized to esaxerenone or placebo for 52 weeks (n=455). Esaxerenone was initiated at 1.25 mg/d and titrated to 2.5 mg/d on the basis of serum potassium monitoring. The primary endpoint was the proportion of patients achieving urinary albumin-to-creatinine ratio remission (<30 mg/g creatinine and ≥30% reduction from baseline on two consecutive occasions).

Results
Overall, 49 (22%) and nine (4%) patients in the esaxerenone and placebo groups, respectively, achieved urinary albumin-to-creatinine ratio remission (absolute difference 18%; 95% confidence interval, 12% to 25%; P<0.001). The percent change in urinary albumin-to-creatinine ratio from baseline to end of treatment was significantly higher with esaxerenone versus placebo (−58% versus 8%; geometric least-squares mean ratio to placebo 0.38, 95% confidence interval, 0.33 to 0.44). There was a significant improvement with esaxerenone versus placebo in time to first remission (hazard ratio, 5.13; 95% confidence interval, 3.27 to 8.04) and time to first transition to urinary albumin-to-creatinine ratio ≥300 mg/g creatinine (hazard ratio, 0.23; 95% confidence interval, 0.11 to 0.48). More patients had a serum potassium level ≥6.0 or ≥5.5 mEq/L on two consecutive measurements in the esaxerenone group (20 [9%]) versus placebo (5 [2%]); these events were asymptomatic and resolved after dosage reduction or treatment discontinuation.

Conclusions
Adding esaxerenone to existing renin-angiotensin system inhibitor therapy in patients with type 2 diabetes and microalbuminuria increased the likelihood of albuminuria returning to normal levels, and reduced progression of albuminuria to higher levels.
Health Leadership Lessons From a Fine Detective
Authors: Arya Dinesh 
Published: November 2020

Objective: To explore lessons on leadership from interactions and success of two very fine detectives – Sherlock Holmes and Dr. Watson Methods: From the review of Sir Arthur Conan Doyle's depiction of Sherlock Holmes, some clear and useful lessons have been drawn for health leaders. An erratic, unsystematic Sherlock Holmes and an analytical, systematic and introspective Dr. Watson provide useful lessons for health leaders on trust, teamwork and how complementary abilities enable the achievement of a great outcome. Conclusions: It is really important for health leaders to not forget the elementary message to remain focused on care provision, enable the provision of care that the community wishes to receive and avoid of the temptation of making decisions without appropriate information set to inform those decisions.
Terapia ocupacional para atendimento odontológico de pacientes especiais
Authors: Spezzia Sérgio 
Published: November 2020

A terapia ocupacional pode ser aplicada no setor odontológico, modificando o ambiente dos consultórios e ambulatórios no que tange à decoração, além de adotar a música no ambiente com o objetivo de minimizar a tensão dos indivíduos no transcorrer dos procedimentos. Com o recurso da terapia ocupacional pode-se ainda fazer emprego do mobiliário, objetivando melhorar a postura dos pacientes nas cadeiras odontológicas. Nesse contexto, torna-se extremamente importante tecer orientações e embasamento à respeito das medidas adotadas e de seus benefícios, orientando tanto o cirurgião dentista como a família dos pacientes. No âmbito odontológico, pacientes especiais podem ser inseridos em nove grupos, são eles: doenças sistêmicas crônicas; doenças infectocontagiosas; condições sistêmicas; anomalias congênitas; deficiência física; deficiência mental; transtornos psiquiátricos; distúrbios comportamentais e distúrbios sensoriais e de comunicação. O terapeuta ocupacional faz uso dos conhecimentos médicos e sociais para dar andamento aos recursos terapêuticos e para atuar na assistênciaodontológica aos pacientes especiais. Busca-se um melhor posicionamento desses pacientes na cadeira do equipo odontológico que se acha instalado para utilização nos atendimentos no decorrer dos procedimentos dentários a serem executados; inibição de movimentos involuntários e a humanização do ambiente, procurando adaptações que sejam condizentes com as necessidades presentes em particular. O objetivo do presente artigo foi verificar a possível inter-relação existente entre terapia ocupacional e a assistência à saúde prestada em odontologia voltada aos pacientes especiais. Realizou-se revisão narrativa de literatura com busca nas bases de dados: Google Acadêmico, PubMED envolvendo levantamento de estudos e artigos que versavam sobre a utilização da terapia ocupacional no atendimento odontológico aos pacientes especiais. No Google Acadêmico buscou-se dar ênfase às pesquisas realizadas entre 2014 e 2019. No PubMED considerou-se todo o acervo disponível na base de dados quando da realização da pesquisa, independentemente da data de publicação dos trabalhos. A utilização da terapia ocupacional a nível odontológico age favoravelmente nos indivíduos, podendo melhorar a qualidade de vida dos pacientes especiais em tratamento.
Monolog Drama ”Matinya Toekang Kritik” Karya Agus Noor (Kajian Teori Reaksi)
Authors: Suprihatien Suprihatien , Damayanti Rini 
Published: November 2020

Tujuan menganalisis monolog drama ini untuk mengetahui sampai sejauh mana suatu ironi dan parodi yang ada pada Monolog Drama ”Matinya Toekang Kritik” Karya Agus Noor dalam Kajian Teori Reaksi. Metode deskriptif kualitatif digunakan untuk pembahasan monolog drama ini dengan objek penelitian berupa naskah drama karya Agus Noor yang berjudul “Matinya Toekang Kritik”. Metode kepustakaan digunakan dalam penelitian ini karena yang diteliti berupa naskah tertulis. Teknik yang digunakan pada penelitian ini berupa teknik baca dan teknik catat. Dasar kajian berupa teori reaksi dalam naskah monolog drama ini. Monolog drama atau lakon ini berani menyampaikan kritik berbagai fenomena kebudayaan, dari mulai masalah ekonomi, sejarah, politik, sampai masalah sosial budaya. Dari kritikan para kritikus ini dapat tersampaikan suatu sindiran mulai yang nakal sampai bernada konyol. Berbagai ironi dan parodi dapat dijumpai pada naskah monolog drama ini.
Molecular characterization of gene encoding halo tolerant amylase of Bacillus paralicheniformis isolated from Jazan region
Authors: Ghazouani Abdullah , El-Gayar Khaled , Abada Emad 
Published: November 2020

Objectives:
This study aims to characterize the gene encoding halo tolerant amylase of bacteria isolated from Jazan region.

Materials and Methods:
Soil samples were collected from several area of Jazan region, KSA. The samples were serially diluted and plateted on starch agar plates. The amylase producing bacteria were detected by iodine test. To determine the halophilic amylase producing bacteria, several colonies were tested for their ability to grow at higher concentrations of NaCl ranging from 7 to 16%. The bacteria was identified by 16S rRNA and the full length amylase gene was fully identified by sequencing using specific primers.

Results:
One bacterial halophilic isolate was able to grow on starch agar medium up to 14% NaCl. The Gram stain of the isolate indicated that it is Gram-positive, bacilli. The 16S rRNA gene homology study showed that the bacterial isolate was identified as Bacillus paralicheniformis. Two specific primers were designed named S1F, S1R, to amplify the amylase gene (AMY) region using PCR and the PCR product was sequenced. The sequencing results showed that the full-length amy gene of B. paralicheniformis was of 1452 encoding 483 amino acids. The expected M.Wt. of the protein expressed is of 55 KDa.

Conclusion:
We report the isolation, identification, and characterization of an isolate of halophilic bacterium isolated from Jazan region. Based on molecular identification, this isolate was identified as Bacillus paralicheniformis. This bacterial strain has an α-amylase gene in its genome and is able to produce extracellular α-amylase. Based on the findings of this work we propose that Bacillus paralicheniformis amy gene could be cloned into expression vector for large scale production.
Leber's hereditary optic neuropathy plus, case report
Authors: Короткова Д.Г. , Карпова М.И. , Буянова Г.В. , Кашко Т.Н. 
Published: November 2020

Наследственная оптическая невропатия Лебера (LHON) - митохондриальное заболевание с атрофией зрительного нерва. Хотя в большинстве случаев LHON других ассоциированных неврологических отклонений нет, сообщалось о случаях LHON plus. В статье представлен анализ клинического случая с проявлением неврологических симптомов в подростковом возрасте.
Leber’s hereditary optic neuropathy (LHON) is a mitochondrial disorder with optic nerve atrophy. Although there are no other associated neurological abnormalities in most cases of LHON, cases of “LHON plus” have been reported. The article presents an analysis of clinical case with the manifestation of neurological symptoms in adolescence.
Sign language prevalence as a source of data for genetic epidemiology of inherited forms of hearing loss
Authors: Романов Г.П. , Пшенникова В.Г. , Лашин С.А. , Соловьев А.В. , Терютин Ф.М. , Сазонов Н.Н. , Хуснутдинова Э.К. , Посух О.Л. , Федорова С.А. , Барашков Н.А. 
Published: November 2020

На основе материалов тотальной переписи населения (2010 г.) проведен анализ распространенности языка жестов в России. Полученные результаты сопоставлены с имеющимися данными о частоте наследуемых форм потери слуха на территории России.
The paper presents the results of an analysis of the prevalence of sign language (SL) in Russia collected during the 2010 total census, as well as their comparison with data on the frequency of hereditary forms of hearing loss.
The frequency of heterozygous carriage of CFTR gene mutations causing the development of cystic fibrosis in a population-based cohort study (ESSE-Vologda)
Authors: Сотникова Е.А. , Климушина М.В. , Киселева А.В. , Скирко О.П. , Курилова О.В. , Дивашук М.Г. , Хлебус Э.Ю. , Козлова В.А. , Покровская М.С. , Сломинский П.А. , Мешков А.Н. , Драпкина О.М. 
Published: November 2020

Целью работы было создание панели для скрининга носительства мутаций, связанных с развитием муковисцидоза (МВ), и апробация панели в популяционной выборке ЭССЕ-Вологда. В исследование вошли 642 участника из популяционной выборки региона Вологды. Детекцию мутаций осуществляли с помощью системы QuantStudio 12K Flex Real-Time PCR. Создана скрининговая панель, включающая 60 мутаций гена CFTR. Среди 642 участников исследования выявлено 23 гетерозиготных носителя 6 мутаций, связанных с развитием МВ, частота носительства составила 3,58% (ДИ 95%: 2,28-5,33%) или 1:28 чеовек. Разработанная панель может быть использована для скрининга гетерозиготных носителей МВ.
The aim of the study was to create a panel for screening the carriers of mutations associated with the development of cystic fibrosis (CF), and testing the panel on 642 participants of the population-based cohort study (ESSE-Vologda). Variants were detected using the QuantStudio 12K Flex Real-Time PCR system. A custom panel that includes 60 CFTR variants was created. Among 642 participants in the study, 23 heterozygous carriers of 6 CFTR mutations were identified. The carrier frequency was 3.58% (CI95%: 2.28-5.33%) or 1:28. The custom panel can be used for screening heterozygous CF carriers.
Development and application targeted NGS panels in the selective screening algorithm for inborn errors of metabolism. An experience of the St. Petersburg Medical and Genetic Center
Authors: Чурюмова Ю.А. , Вохмянина Н.В. , Шляга С.В. , Вавилова Т.В. , Симакова Т.С. , Зайцева М.А. , Павлов А.Е. 
Published: November 2020

Наследственные болезни обмена веществ представляют собой обширный класс генетических заболеваний и вносят значительный вклад в детскую заболеваемость, при этом их диагностика с использованием биохимических методов зачастую вызывает затруднения. В СПбГКУЗ МГЦ были разработаны и внедрены три панели для секвенирования 88 генов, ответственных за развитие трех групп наследственных болезней обмена (НБО), и протестировано 84 ребенка, у которых данные заболевания были заподозрены по данным тандемной масс-спектрометрии (ТМС), либо по наличию клинических симптомов. У 6 детей методом NGS полностью установлена генетическая причина заболевания. Патогенные мутации выявлялись значительно чаще при повышении биохимических маркеров, демонстрируя ведущую роль предварительного биохимического скрининга в проведении NGS анализа. NGS значительно повышает результативность клинической диагностики НБО. Биохимическое тестирование и NGS играют взаимодополняющие роли, и их комплексное использование в алгоритме селективного скрининга позволяет повысить точность диагностики НБО.
Inborn errors of metabolism are an extensive class of genetic diseases and contribute significantly to childhood morbidity, and their diagnosis using biochemical methods is often difficult. Three panels for sequencing of 88 genes responsible for the development of three groups of inborn errors of metabolism (IEM) were developed and introduced in St.Petersburg Medical and Genetic Center and 84 children were tested for which these diseases were suspected by tandem mass-spectrometry or by the presence of clinical symptoms. In 6 children, the NGS method fully established the genetic cause of the disease. Pathogenic mutations were detected significantly more frequently with increased biochemical markers, demonstrating the leading role of pre-biochemical screening in performing NGS analysis. NGS significantly improves the clinical diagnostic effectiveness of IEM. Biochemical testing and NGS play complementary roles and their complex use in selective screening algorithm allows to increase accuracy of IEM diagnostics.