bluuebunny/crossref_metadata_2025_split huggingface dataset:
Humic acids trigger the weak acids stress response in maize seedlings
Authors: Baía Daiane Carvalho , Olivares Fábio L. , Zandonadi Daniel B. , de Paula Soares Cleiton , Spaccini Riccardo , Canellas Luciano P. 
Published: December 2020

AbstractBackgroundPlants primed by humic acids showed physiological and molecular response against different abiotic stresses without the presence of stressor agents (salinity, drought, heavy metal toxicity). It is plausible that humic acids themselves can act as chemical priming substances in plants. We hypothesized that humic acids can trigger the weak acids stress response in cell plants acidifying the cytosol and thus eliciting the transduction signalling response cascade.MethodsThe dose–response curves of maize seedlings roots with different concentrations of humic, acetic and salicylic acids determined the most active and inhibitory concentration. These data were further used to evaluate changes on intracellular pH using BCECF-AM probe (2,7-bis(2-carboxyethyl)-5(and 6)-carboxyfluorescein, acetoxymethyl ester) and differential transcription level of genes related to weak stress response in plants by qPCR real time.ResultsHumic acids like short chain organic acids decrease the intracellular pH showed by the increased fluorescence of BCECF probe. The drop in cytosolic pH promoted by humic acids was not transient. We observed a high level of protein kinases related to cell energy-sensing and transcription factors associated to transduction of stress signalling.ConclusionThe humic acids can be considered as a chemical priming agent, since in the appropriate concentration they can induce the typical plant abiotic stress response of weak acids inducing plant acclimation and enhancing the abiotic stress tolerance.
Campbell biology (edited by Lisa Urry, Michael Cain, Steven Wasserman, Peter Minorsky and Jane Reece)
Authors: Shen Gangxu 
Published: December 2020

AbstractCampbell Biology is divided into eight units and 56 chapters. The organization and size of this book are appropriate and easy for first-year university students and help them to learn and digest the content. Campbell Biology is currently among the best biology books and it is listed with the best shelling textbooks. Campbell Biology is mainly for first-year university students, but it is also an important book for postgraduate medical examinations. Moreover, some high school students may use it as an essential reference book. In its current edition, the latest information in various fields has been added, such as the basal body, which was previously called the 9*3 type microtube arrangement but now has been renamed as the 9 + 0 type in Chapter 6. The updates in molecular biology are closer to the current situation, such as the addition of information on next-generation sequencing and CRISPR/Cas9 in Chapter 20. This content can enable readers to acquire the latest knowledge. Reading this book and understanding the information presented in its pages is very helpful for the future life science professionals. Thus, Campbell Biology is very valuable textbook in the field of biology.
A case of colorectal large cell neuroendocrine carcinoma accompanied by disseminated peritoneal leiomyomatosis
Authors: Suga Kunihiko , Ogawa Hiroomi , Sohda Makoto , Katayama Chika , Ozawa Naoya , Osone Katsuya , Okada Takuhisa , Shiraishi Takuya , Katoh Ryuji , Sano Akihiko , Sakai Makoto , Yokobori Takehiko , Shirabe Ken , Saeki Hiroshi 
Published: December 2020

Abstract
Background
Neuroendocrine carcinomas (NECs) of the colon are among the rarest types of colorectal cancers. Among these, large cell type neuroendocrine carcinoma (LCNEC) is particularly rare. Colorectal NEC is an aggressive disease, and there are few reports of long-term survivors. Here, we report a case of LCNEC accompanied by disseminated peritoneal leiomyomatosis that was difficult to diagnose.

Case presentation
The case involves a 62-year-old female found to be positive for fecal occult blood by medical examination. An endoscopy revealed a tumor in the ascending colon, and the biopsy revealed poorly differentiated cancer. Abnormal FDG accumulation with peritoneal thickening was visible on 18F-fluorodeoxyglucose positron-emission tomography (FDG-PET) and suspected to be peritoneal dissemination. Laparoscopic ileocecal resection was performed for the tumor of the ascending colon with abdominal wall invasion. At that time, numerous intra-abdominal nodules were observed, indicating peritoneal dissemination. The pathological diagnosis of the primary lesion was LCNEC, and the patient requested to undergo total peritoneal resection. After one course of chemotherapy with irinotecan plus cisplatin, she underwent total peritoneal resection, uterine annex resection, left inguinal lymph node resection, and intra-abdominal hyperthermic intraperitoneal chemotherapy with mitomycin C. Because a postoperative pathological examination revealed that the intra-abdominal nodules were leiomyomas, we diagnosed the patient with disseminated peritoneal leiomyomatosis. The left inguinal lymph node was diagnosed with a metastatic tumor. In summary, the final diagnosis was LCNEC in the ascending colon with inguinal lymph node metastasis. Postoperative chemotherapy has been administered to date. She is currently 18 months post-primary surgery and 15 months post-peritonectomy without apparent recurrence or metastatic findings.

Conclusion
We experienced a case of Stage IVa colorectal LCNEC accompanied by disseminated peritoneal leiomyomatosis. Although the prognosis is generally poor, multidisciplinary treatment for advanced colorectal LCNEC may result in a favorable outcome for some patients. If peritoneal dissemination is suspected during operation, sampling of the nodule to confirm the pathological diagnosis is advisable.
A bio-economic simulation study on the association between key performance indicators and pluck lesions in Irish farrow-to-finish pig farms
Authors: Calderón Díaz Julia Adriana , Rodrigues da Costa Maria , Shalloo Laurence , Niemi Jarkko K. , Leonard Finola Catherine , Crespo-Piazuelo Daniel , Gasa Josep , García Manzanilla Edgar 
Published: December 2020

Abstract
Background
Pluck lesions are associated with decreased performance in grower-finisher pigs, but their economic impact needs to be further investigated. This study aimed to identify the main pluck lesions and the cut-off value for their prevalence, associated with changes in average daily gain (ADG) during the wean-to-finish period, to simulate their effects on economic performance of farrow-to-finish farms. Pigs (n = 162 ± 51.9 per farm) from 56 farrow-to-finish farms were inspected at slaughter and the prevalence of enzootic pneumonia-like lesions, pleurisy, lung scars, abscesses, pericarditis, and liver milk spots was estimated. For each farm, annual performance indicators were obtained. Regression trees analysis (RTA) was used to identify pluck lesions and to estimate cut-off values for their prevalence associated with changes in ADG. Different scenarios were simulated as per RTA results and economic and risk analyses were performed using the Teagasc Pig Production Model. Risk analysis was performed by Monte Carlo sampling using the Microsoft Excel add-in @Risk with 10,000 iterations.

Results
Pleurisy and lung scars were the main lesions associated with changes in ADG. Three scenarios were simulated based on RTA results: a 728 sow farrow-to-finish farm with prevalence of i) pleurisy < 25% and lung scars < 8% (LPLSC; ADG = 760 g); ii) pleurisy < 25% and lung scar ≥8% (LPHSC; ADG = 725 g) and iii) pleurisy ≥25% (HP; ADG = 671 g). The economic analysis showed increased feed and dead animals for disposal costs, and lower sales in the HP and LPHSC scenarios than in the LPLSC scenario; thereby reducing gross margin and net profit. Results from the risk analysis showed lower probability of reaching any given level of profit in the HP scenario compared with the LPHSC and LPLSC scenarios.

Conclusion
Under the conditions of this study, higher prevalence of pleurisy and lung scars were associated with decreased ADG during the grower-finisher period and with lower economic return in the simulated farms. These results highlight the economic benefits and importance of preventing and/or controlling respiratory disease.
A century of Hereditas: from local publication to international journal
Authors: Tunlid Anna , Kristoffersson Ulf , Åström Fredrik 
Published: December 2020

Abstract
Background
The Mendelian Society of Lund launched Hereditas in 1920. The purpose of this article is to give an overview of Hereditas’s hundred-year existence, focusing on the conditions for a learned society to publish a scientific journal, and the journal’s importance for the publication and dissemination of genetic research. The article focuses on the historical development of the journal and analyses how the content and orientation of research published in Hereditas have changed over the years.

Methods
The historical study is based on the collation and interpretation of archival material, mainly held in the Mendelian Society’s archive, which includes the Hereditas archive. The bibliometric analyses are based on bibliographic metadata from Web of Science (WoS). Together with descriptive statistics, co-citation analyses were performed by using BibExcel, in combination with the clustering and visualisation tool VOSviewer. Journals with articles citing Hereditas articles were identified as a complement to the co-citation analyses.

Results
The history of the journal falls into three main periods: a local period, 1920–1959, when Hereditas was primarily intended for Swedish geneticists; a Scandinavian period, 1960–1988, when Hereditas was the official journal of the Scandinavian Association of Geneticists; and an international period from 1989 onwards. The original decision that Hereditas should cover genetic research with no particular specialisation was retained throughout. Its publications demonstrate the continuing presence of genetic research on plants and animals, albeit with a shifting focus, while human genetics emerged slowly and reached its peak in the period 1960–1988.

Conclusion
In the hundred years of Hereditas’s existence, the publishing landscape has changed dramatically, including a far greater number of specialist journals, changes to the academic merit system, new commercial models for publishing, and digitalisation. Over the years, the journal’s survival has therefore been dependent on the strong commitment of its owners and an ability to adapt to changing conditions.
A new insight into the treatment of renal anemia with HIF stabilizer
Authors: Kuriyama Satoru , Maruyama Yukio , Honda Hirokazu 
Published: December 2020

AbstractThe long-term clinical experiences with recombinant human erythropoietin (rHuEPO) and its analog derivatives have clearly proven that correction of anemia with erythropoiesis stimulating agent (ESA) not only reduces blood transfusion and improves patients’ QOL but has multiple benefits for the concurrent complications of CKD such as Cardio-Renal–Anemia (CRA) syndrome and/or malnutrition-inflammation-atherosclerosis (MIA) syndrome.Unlike ESA, the newly available agent, hypoxia-inducible factor (HIF) stabilizer, stimulates endogenous erythropoietin (EPO) by mimicking hypoxia with HIF prolyl hydroxylase domain enzyme (HIF-PHD) inhibition. The phase 2 and 3 clinical studies have shown that HIF stabilizers are as efficacious as ESA in ameliorating renal anemia. Whether the same clinical benefits on CRA and MIA syndrome hold true in patients given HIF stabilizers is a matter for future debate. Given that HIF stabilizers act on the multiple target genes, the use of this novel agent may lead to unwanted adverse events.Launching HIF stabilizers into the treatment of renal anemia provokes a concern about how this alternative treatment will be taken up in the daily clinical practice. However, guideline-oriented strategies on how to use HIF stabilizer is not available at this limited point due to scant clinical information. Nevertheless, this opinion-based review provides a future insight into the management of renal anemia with HIF stabilizer by reference to the past experiences with ESA. HIF stabilizers can preferably be indicated for CRA syndrome at pre-dialysis stage, ESA resistant anemia at advanced CKD stage, and perhaps for dysregulated iron metabolism akin to MIA syndrome in patients on dialysis.
Widest geographic distribution of a shallow and mesophotic antipatharian coral (Anthozoa: Hexacorallia): Antipathes grandis VERRILL, 1928 – confirmed by morphometric and molecular analyses
Authors: Gress Erika , Opresko Dennis M. , Brugler Mercer R. , Wagner Daniel , Eeckhaut Igor , Terrana Lucas 
Published: December 2020

AbstractWe provide the first record of the shallow and mesophotic (< 150 m depth) antipatharian coral Antipathes grandis VERRILL, 1928 from the Indian Ocean. First described from Hawaii, A. grandis was recently found on a mesophotic coral reef in SW Madagascar. Its identity was confirmed by means of morphological and molecular analyses (cytochrome c oxidase subunit I). These findings not only expand our knowledge of the potential geographic range of antipatharian corals, but also challenge the belief that only deep-sea (> 200 m depth) benthic taxa have such wide geographic distributions.
The effectiveness of inhaled Cannabis flower for the treatment of agitation/irritability, anxiety, and common stress
Authors: Stith Sarah S. , Li Xiaoxue , Diviant Jegason P. , Brockelman Franco C. , Keeling Keenan S. , Hall Branden , Vigil Jacob M. 
Published: December 2020

Abstract
Background
An observational research design was used to evaluate which types of commonly labeled Cannabis flower product characteristics are associated with changes in momentary feelings of distress-related symptoms.

Methods
We used data from 2306 patient-directed cannabis administration sessions among 670 people who used the real-time Cannabis effects recording software, Releaf App, between June 6, 2016, and February 23, 2019, for tracking the effects of Cannabis flower consumption. Fixed effects multivariable panel regression techniques were used to establish overall relief by symptom type and to determine which labeled product characteristics (e.g., subspecies/subtype, inhalation method, and major cannabinoid contents) showed the strongest correlation with changes in momentary feelings of agitation/irritability, anxiety, and stress, along with experienced side effects.

Results
In total, a decrease in symptom intensity levels was reported in 95.51% of Cannabis usage sessions, an increase in 2.32% of sessions, and no change in 2.16% of sessions. Fixed effects models showed, on average, respondents recorded a maximum symptom intensity reduction of 4.33 points for agitation/irritability (SE = 0.20, p < 0.01), 3.47 points for anxiety (SE = 0.13, p < 0.01), and 3.98 for stress (SE = 0.12, p < 0.01) on an 11-point visual analog scale. Fixed effects regressions showed that, controlling for time-invariant user characteristics, mid and high tetrahydrocannabinol (THC) levels were the primary independent predictor of increased symptom relief, and that when broken out by symptom type, this effect was only statistically significant for our largest sample of users, those reporting anxiety rather than agitation/irritability or stress. Cannabidiol (CBD) levels were generally not associated with changes in symptom intensity levels. In a minority of cannabis use sessions (< 13%), cannabis users reported anxiogenic-related negative side effects (e.g., feeling anxious, irritable, paranoid, rapid pulse, or restless), whereas in a majority of sessions (about 66%), users reported positive anxiolytic side effects (e.g., feeling chill, comfy, happy, optimistic, peaceful, or relaxed).

Conclusions
The findings suggest the majority of patients in our sample experienced relief from distress-related symptoms following consumption of Cannabis flower, and that among product characteristics, higher THC levels were the strongest predictors of relief.
Diffusion-thermo and thermal-diffusion effects with inclined magnetic field on unsteady MHD slip flow over a permeable vertical plate
Authors: Oyekunle T. L. , Agunbiade S. A. 
Published: December 2020

AbstractIn this study, various fluid physical quantities effects such as diffusion-thermo, thermal-diffusion, thermal radiation, viscous dissipation, inclined magnetic field on unsteady MHD slip flow over a permeable vertical plate are considered. The coupled and nonlinear partial differential governing equations consisting of momentum, energy and species equations are reduced to ordinary differential equations using perturbation technique. The resulted coupled, nonlinear ordinary differential equations are solved by using collocation method with the aid of assumed Legendre polynomial. The impacts of different physical parameters on fluid properties are discussed and presented both graphically and tabularly. Both Dufour and Soret have the tendency of enhancing velocity profiles.
Most promising solid dispersion technique of oral dispersible tablet
Authors: Nikam Vikrant K. , Shete Shubham K. , Khapare Jyoti P. 
Published: December 2020

AbstractBackgroundThe most common problem about conventional dosage form is dysphagia (difficulty in swallowing). So, we design a new approach in a conventional dosage form which is oral dispersible tablet. Oral dispersible tablet is also called as mouth dissolving tablet, fast dissolving tablet, or oral disintegrating tablet. Oral dispersible tablet has advantage as it quickly disintegrates into saliva when it is put on the tongue. The faster the drug disintegrates or is dissolved, the faster the absorption and the quicker the therapeutic effect of drug will be attained.Main textThis review article focuses on the progress in methods of manufacturing and various latest technologies involved in the development of oral disintegrating tablet. The solid dispersion technique is one of the novel techniques to manufacturing the oral dispersible tablet. Solid dispersion is basically a drug polymer two component system.ConclusionThis review article focuses on advantages, disadvantages, materials used as carrier for solid dispersions, methods of preparation of solid dispersion, classification of solid dispersion, promising drugs that can be incorporated into oral disintegrating tablet by solid dispersion techniques, and recent research in solid dispersion technique using polymers as carriers.
Characterization and toxicity evaluation of degradation products of febantel
Authors: Mahajan Anand A. , Marathe Amey M. , Jarande Suvarna S. , Pissurlenkar Raghuvir , Gawande Vandana T. 
Published: December 2020

Abstract
Background
The aim of the present work was to determine potential toxicity of degradation products of febantel generated under different stress conditions mentioned in guideline Q1A (R2) laid down by International Council for Harmonization (ICH). The stability behavior of febantel was studied by subjecting it to hydrolytic, oxidative, photolytic and thermal forced degradation conditions.

Results
Five degradation products (DPs) were observed which were resolved using high-performance liquid chromatography (HPLC) and characterized by LC-MS/MS using positive mode of electrospray ionization. The chromatographic separation was carried out on Hypersil® BDS C18 (150 × 4.6 mm, 5 μm) column. Optimum resolution was obtained using ammonium formate buffer (10 mM, pH 3.5) and acetonitrile programmed in gradient elution mode at 281.0 nm using photodiode array detector.

Conclusion
The drug was found susceptible to degradation under all the stress conditions except thermal and oxidative stress. Five major unknown degradation products DP–I, DP–II, DP–III, DP–IV, and DP–V generated under photolytic, alkali, and acidic stress condition were identified and characterized by LC-MS/MS. The drug and identified degradation products were screened for prediction of in-silico toxicity using software viz. Swiss ADME, OSIRIS Property Explorer and Pro Tox II which indicated overall no toxicological concerns.

Graphical abstract
Upper gastrointestinal series in children: what surgeons need to know
Authors: Hamed Eman Ahmed , Mohammad Shaimaa Abdelsattar , Rawash Eldieb Leila M. , Shalaby Mennatallah Hatem 
Published: December 2020

Abstract
Background
Upper gastrointestinal (UGI) series is the standard imaging tool for diagnosis of structural and functional abnormalities affecting the esophagus, stomach, and proximal small bowel. The aim of this study was to delineate the clinical indications for UGI series in children that are associated with the presence of significant radiological abnormalities aiming for more standardized care for those patients.

Results
UGI series of 118 patients was analyzed with calculation of clinical score. Vomiting was the most frequent primary complaint (63.6%), followed by dysphagia and recurrent chest infection. Forty-nine patients had positive upper GI findings (42%). The most detected abnormality was related to the stomach and duodenum (21.4%). Variable degrees of idiopathic gastroesophageal reflux were detected in 16 patients (13.6%). Patients with clinical score 2 or more had significantly more prevalence of abnormal findings (p = .001). Vomiting (especially when bilious), underweight, aspiration, and recurrent chest infection were strong predictors of abnormal findings on upper GI series (p = 0.007, 0.001, 0.009, and 0.001, respectively) and increased the diagnostic yield of upper GI series for detection of abnormalities by 3.48, 9.6, 4, and 4.12 times, respectively.

Conclusion
Diagnostic yield of UGI series was relatively higher in patients having two or more symptoms (clinical score 2 or more) and in children with bilious vomiting, aspiration and underweight, or repeated chest infection.
A comparative study of the voice-related quality of life among Egyptian elderly with and without voice complaints
Authors: Mahmoud Nesreen Fathi , Zahran Huda , Abdelmonam Sherif 
Published: December 2020

Abstract
Background
This study focuses on the self-perception of the voice in the elderly as assessed by the Voice-Related Quality of Life (V-RQOL) questionnaire. This work aimed to compare differences in the voice-related quality of life outcomes between (1) elderly with and without voice disorders, (2) female and male elderly with voice disorders, and (3) different types of voice disorders, and to explore the correlation between the V-RQOL and perceptual analysis done by the clinician. Forty-three dysphonic and 44 non-dysphonic elderly filled out the Voice-Related Quality of Life (V-RQOL) protocol that analyzes the impact of dysphonia on life quality. Vocal perceptual assessment of each subject with dysphonia was made by three voice therapists, followed by a flexible nasofibrolaryngoscope.

Results
A significant statistical difference was found between the means of total V-RQOL scores and its subdomains for each group (dysphonic and non-dysphonic). No significant differences were found between male and female elderly with dysphonia. The statistical analysis showed a significant correlation with the vocal assessment made by the clinicians and the V-RQOL self-assessment made by the subjects.

Conclusions
This study provides valuable information regarding the risk factors that contribute to vocal quality in the elderly population. Our results revealed that different types of voice disorders are common among the elderly population with significant negative effects on quality of life. It was observed that the poorest score on the V-RQOL was for functional voice disorders, followed by neoplastic lesions, whereas MAPLs had the best score on the V-RQOL.