bluuebunny/crossref_metadata_2025_split huggingface dataset:
RhB‐Embedded Zirconium–Naphthalene‐Based Metal–Organic Framework Composite as a Luminescent Self‐Calibrating Platform for the Selective Detection of Inorganic Ions
Authors: Zhang Zhinan , Wei Zihao , Meng Fanyu , Su Jiali , Chen Dashu , Guo Zhifen , Xing Hongzhu 
Published: December 2019

AbstractA series of dye@MOF composites were synthesized through in situ encapsulation of luminous rhodamine B (RhB) molecules into a blue‐emissive zirconium–naphthalene‐based metal–organic framework (Zr‐MOF). The fabricated RhB@Zr‐MOF composites exhibit tunable dual‐emissive characteristics due to the process of resonant energy transfer from Zr‐MOF to RhB. Notably, one of the RhB@Zr‐MOF composites (R@D3) exhibited a weak emission at 420 nm and a strong emission at 607 nm, for which the two emissions possess large distinctions in location and intensity and can be referenced with each other in sensing analytes. By using relative fluorescence intensity instead of their absolute fluorescence intensity as the detection signals, R@D3 served as a built‐in self‐calibrated platform to selectively detect Fe3+ and Cr2O72− ions in water. Compared with the pristine Zr‐MOF, the R@D3 composite shows enhanced sensing selectivity to Fe3+ and higher sensibility to Cr2O72−. This study displays the advantages of combining organic dyes with robust Zr‐MOFs in tuning fluorescence and sensing performance.
Kemp Elimination Reaction Catalyzed by Electric Fields
Authors: Acosta‐Silva Carles , Bertran Joan , Branchadell Vicenç , Oliva Antoni 
Published: December 2019

AbstractThe Kemp elimination reaction is the most widely used in the de novo design of new enzymes. The effect of two different kinds of electric fields in the reactions of acetate as a base with benzisoxazole and 5‐nitrobenzisoxazole as substrates have been theoretically studied. The effect of the solvent reaction field has been calculated using the SMD continuum model for several solvents; we have shown that solvents inhibit both reactions, the decrease of the reaction rate being larger as far as the dielectric constant is increased. The diminution of the reaction rate is especially remarkable between aprotic organic solvents and protic solvents as water, the electrostatic term of the hydrogen bonds being the main factor for the large inhibitory effect of water. The presence of an external electric field oriented in the direction of the charge transfer (z axis) increases it and, so, the reaction rate. In the reaction of the nitro compound, if the electric field is oriented in an orthogonal direction (x axis) the charge transfer to the NO2 group is favored and there is a subsequent increase of the reaction rate. However, this increase is smaller than the one produced by the field in the z axis. It is worthwhile mentioning that one of the main effects of external electric fields of intermediate intensity is the reorientation of the reactants. Finally, the implications of our results in the de novo design of enzymes are discussed.
Influence of the Synthesis Route on the Properties of Hybrid NiO–MnCo2O4–Ni6MnO8 Anode Materials and their Electrochemical Performances
Authors: Zhu Jie , Cao Guolin , Zhou Yuan , Li Yunjiao , Zheng Junchao , Zhang Dianwei 
Published: December 2019

AbstractNew materials with different morphologies, nanostructures, and components can have structural advantages for application in materials science. Multicomponent‐active hybrid nanostructured materials are among the best candidates for application in electrode materials. Spray pyrolysis and solvothermal synthesis are two popular methods for the preparation of multicomponent‐active hybrid nanostructured materials. In this study, the two types of NiO‐MnCo2O4‐Ni6MnO8 hybrid anode materials for use in lithium‐ion batteries were synthesized by two different methods (spray pyrolysis and solvothermal synthesis), and the differences in their physical and electrochemical properties were compared. The two types of anode material exhibited the same hierarchical hybrid composition, but some different physical characteristics, which affected their electrochemical performance.
Compact memristor‐based ultra‐wide band chirp pulse generator
Authors: Barraj Imen , Bahloul Mohamed , Fouda Mohamed E. , Masmoudi Mohamed 
Published: December 2019

SummaryIn this letter, a memristor‐based chirp pulse generator circuit is introduced for ultra‐wideband transceivers for the first time. The proposed generator is built using memristor‐controlled ring oscillator. Memristor is used to replace the bulky components in the chirp pulse generation such as surface acoustic wave filters and reactive components (capacitor and inductors), which reflects a huge reduction in area and power. The chirp pulse frequency of the proposed circuit varies linearly with time over the pulse duration due to the change in the memristance. The proposed circuit is mathematically analyzed and designed using 65‐nm complementary metal‐oxide‐semiconductor (CMOS) technology. The 500‐MHz bandwidth is demonstrated using a 32‐ns pulse width complying with the FCC regulations. The power consumption is 84 μW with a 10‐MHz pulse repetition frequency (PRF).
Integrating dynamic environmental predictors and species occurrences: Toward true dynamic species distribution models
Authors: Milanesi Pietro , Della Rocca Francesca , Robinson Robert A. 
Published: December 2019

AbstractWhile biological distributions are not static and change/evolve through space and time, nonstationarity of climatic and land‐use conditions is frequently neglected in species distribution models. Even recent techniques accounting for spatiotemporal variation of species occurrence basically consider the environmental predictors as static; specifically, in most studies using species distribution models, predictor values are averaged over a 50‐ or 30‐year time period. This could lead to a strong bias due to monthly/annual variation between the climatic conditions in which species' locations were recorded and those used to develop species distribution models or even a complete mismatch if locations have been recorded more recently. Moreover, the impact of land‐use change has only recently begun to be fully explored in species distribution models, but again without considering year‐specific values. Excluding dynamic climate and land‐use predictors could provide misleading estimation of species distribution. In recent years, however, open‐access spatially explicit databases that provide high‐resolution monthly and annual variation in climate (for the period 1901–2016) and land‐use (for the period 1992–2015) conditions at a global scale have become available. Combining species locations collected in a given month of a given year with the relative climatic and land‐use predictors derived from these datasets would thus lead to the development of true dynamic species distribution models (D‐SDMs), improving predictive accuracy and avoiding mismatch between species locations and predictor variables. Thus, we strongly encourage modelers to develop D‐SDMs using month‐ and year‐specific climatic data as well as year‐specific land‐use data that match the period in which species data were collected.
Nrf2 expression driven by Foxp3 specific deletion of Keap1 results in loss of immune tolerance in mice
Authors: Klemm Patricia , Rajendiran Anandhi , Fragoulis Athanassios , Wruck Christoph , Schippers Angela , Wagner Norbert , Bopp Tobias , Tenbrock Klaus , Ohl Kim 
Published: December 2019

AbstractThe transcription factor Nrf2 regulates oxidative stress responses. However, the specific function of Nrf2 in Tregs, the central regulators of immune homeostasis, is unclear. Here, we report an unexpected but important role of Nrf2 in Tregs. Nrf2 expression driven by Foxp3 specific deletion of Keap1 resulted in an autoinflammatory phenotype with enhanced effector T cell activation and immune cell infiltrates in the lung. While early postnatal death of mice with Foxp3 specific deletion of Keap1 was most probably due to ectopic Foxp3cre expression and subsequent Keap1 deletion in epithelial cells, bone marrow chimeras suggest that Nrf2 activation intrinsically in Tregs contributes to a loss of Treg cells and diminished peripheral tolerance. Moreover, Nrf2 activation was associated with a loss of Foxp3 expression, but an enhanced glucose uptake and mTOR activity in Tregs, thus mimicking a metabolic phenotype that is associated with impaired lineage stability and cell functioning.
1,4‐Benzoquinone Derivatives for Enhanced Bioelectrocatalysis by Fructose Dehydrogenase from Gluconobacter Japonicus: Towards Promising D‐Fructose Biosensor Development
Authors: Voitechovič Edita , Vektarienė Aušra , Vektaris Gytis , Jančienė Regina , Razumienė Julija , Gurevičienė Vidutė 
Published: December 2019

AbstractD‐fructose amount in food and beverages should be carefully controlled in order to avoid its overconsumption by human, which can lead to various metabolic diseases. Thus, the development of a low‐cost and portable (bio)sensors, which realize the on‐site monitoring of D‐fructose, is still highly required. In this work, we proposed several reagentless bioelectrochemical systems (BioESs) based on fructose dehydrogenase EC1.1.99.11 from Gluconobacter japonicus (FDH) and on 2‐arylamine‐1,4‐benzoquinone (ABQ) derivatives as a platform for the development of D‐fructose electrochemical biosensor. To design a reliable and easily reproducible BioES, we used the simple physical sorption as the electrode modification strategy for ABQs and FDH immobilization that is suitable for low‐cost mass production of the biosensors by standard microfabrication techniques. To choose the most suitable ABQ compounds for BioESs design, we proposed a novel theoretical approach of potential ET mediator evaluation through its electrochemical properties. A set of newly synthesized and of previously applied ABQs was characterized both electrochemically and using the density functional theory (DFT). It was shown that results obtained by quantum chemical analysis were in a good agreement with the ABQ characteristics obtained in electrochemical measurements. We suggest that the calculated local ionization energy values and theoretical redox potential obtained by DFT are a powerful tool for prediction of ABQs electrochemical properties. The performance of the BioESs with FDH under study was determined by electrochemical and electronic properties of ABQ derivatives and FDH orientation and stabilization on the electrode surface. The most promising BioES was based on carbon paste electrodes and 2‐(3‐nitro(phenyl)amino)‐ cyclohexa‐2,5‐dien‐1,4‐dione as ET mediator, which accelerated FDH catalysis and improved its stability better, then other studied ABQs. The bioelectrocatalytic process was characterized by initial apparent maximum current density of 7.1 μA cm−2 at the optimal conditions (+400 mV vs. Ag/AgCl, McIlvaine's buffer, pH 5.0 and 20 °C). The findings of this research open new possibilities for development of cost‐effective biosensors with FDH and, potentially, with other redox enzymes.
An assessment of the interaction for three Chrysanthemum indicum flavonoids and α‐amylase by surface plasmon resonance
Authors: Li Pao , Huang Zhao , She Yin , Qin Si , Gao Wanru , Cao Yanan , Liu Xia 
Published: December 2019

AbstractThis study evaluated the interaction of Chrysanthemum indicum (CI) flavonoids (luteolin, acacetin, and buddleoside) with α‐amylase. Surface plasmon resonance (SPR) assay showed their equilibrium dissociation constants (KD) are 1.9695 ± 0.12, 2.9240 ± 0.20, and 3.2966 ± 0.08 mM at pH 6.0, respectively. Furthermore, their binding affinities were influenced by KCl, MgCl2, and CaCl2. Enzymatic kinetic studies revealed that three flavonoids exhibited noncompetitive α‐amylase inhibitory activity. The inhibitory sequence is luteolin > acacetin > buddleoside, which was in accordance with the results of binding affinity from SPR. 1,1‐diphenyl‐2‐picryl hydrazyl radical assay demonstrated that antioxidant activities of three flavonoids were inhibited significantly with α‐amylase. Meanwhile, the study reveals that hydroxyl on C′‐4, C′‐5, and C‐7 of flavonoids play an important role on the interaction of three flavonoids with α‐amylase. Also, SPR could be used as sensor for rapid screening inhibitors of α‐amylase and provide useful information for the application of C. indicum flavonoids in food and pharmaceutical area.
Preventive effect of ursolic acid derivative on particulate matter 2.5‐induced chronic obstructive pulmonary disease involves suppression of lung inflammation
Authors: Li Cuini , Chen Junxian , Yuan Weiwei , Zhang Wei , Chen Hong , Tan Hongtao 
Published: December 2019

AbstractRespiratory diseases like chronic obstructive pulmonary disease (COPD) are associated with the presence of particulate matter 2.5 (PM2.5) in the air. In the present study, the effect of synthesized ursolic acid derivatives on mice model of PM2.5‐induced COPD was investigated in vivo. The mice model of COPD was established by the administration of 25 μL of PM2.5 suspension through intranasal route daily for 1 week. The levels of oxidative stress markers and inflammatory cytokines like tumor necrosis factors‐α and interleukin‐6 in the mice bronchoalveolar fluids increased markedly on administration with PM2.5. However, treatment with ursolic acid derivative caused a significant suppression in PM2.5‐induced increase in oxidative stress markers and inflammatory cytokines in dose‐dependent manner. Hematoxylin and eosin staining showed excessive inflammatory cell infiltration in pulmonary tissues in mice with COPD. The inflammatory cell infiltration was inhibited on treatment of the mice with ursolic acid derivative. The ursolic acid derivative treatment increased level of superoxide dismutase in mice with COPD. The lung injury induced by PM2.5 in mice was also prevented on treatment with ursolic acid derivative. Thus, ursolic acid derivative inhibits pulmonary tissues damage in mice through suppression of inflammatory cytokine and oxidative enzymes. Therefore, ursolic acid derivative can be of therapeutic importance for treatment of PM2.5‐induced COPD.
Green and ecofriendly synthesis of indole‐condensed benzimidazole chalcones in water and their antimicrobial evaluations
Authors: Dasari Gopala K. , Sunkara Satyaveni , Gadupudi Purna C. R. 
Published: December 2019

AbstractAn ecofriendly synthesis of novel benzimidazole‐substituted indole derivatives catalyzed by ZnO nanoparticles has been developed from simple condensation of acetyl benzimidazole and indole‐3‐carbaldehyde followed by Michael addition of indoles. This ZnO Nano particles (NP)‐catalyzed reaction has been carried out using both step‐wise and one‐pot methods, and both these methods provide excellent yield of product and less reaction time in water. All the synthesized compounds were characterized by Infrared spectroscopy (IR), Proton Nuclear Magnetic Resonance spectroscopy (Proton NMR), Carbon‐13 Proton Nuclear Magnetic Resonance spectroscopy (13C‐NMR), and mass analysis and screened for their antibacterial activity against clinical strains that include Gram‐positive and Gram‐negative bacteria.
The characterization of column heating effect in nanoflow liquid chromatography mass spectrometry (nanoLC‐MS)–based proteomics
Authors: Li Bolin , Guo Fang , Hu Hao , Liu Ping , Tan Minjia , Pan Jianyi , Zhai Linhui 
Published: December 2019

AbstractColumn heating strategy is often applied in nano–high‐performance liquid chromatography–mass spectrometer (nanoHPLC‐MS) platform for enhancing the analytical efficiency of peptides or proteins. Nonetheless, the influence effects of column heating in peptides or proteins identification still lack of deep understanding. In this study, a systematic comparison of room temperature (RT) and column heating of nanoHPLC was done. Based on the data, under column heating condition, the backpressure of nanoHPLC can be decreased. Due to the increase of resolution, the peak widths of precursor ion were narrowed. As a result, in MS/MS data acquisition part, more time was spared for MS1 detecting and MS2 fragmenting, which eventually resulted in increased identification of peptides and proteins. Moreover, we also proposed the application scope of column heating by evaluating its influence on sample detection. On one hand, column heating significantly increased the identification of membrane proteins due to more efficient elution of highly hydrophobic peptides compared with RT. On the other hand, heating was not suitable for analyzing short or/and hydrophilic peptides with low retention time, which would be eluted out during sample loading process under high temperature and missed by mass spectrometric detection. In conclusion, our study provides a reference for rational application of column heating in proteomics research.
Mitoprotective therapy prevents rapid, strain‐dependent mitochondrial dysfunction after articular cartilage injury
Authors: Bartell Lena R. , Fortier Lisa A. , Bonassar Lawrence J. , Szeto Hazel H. , Cohen Itai , Delco Michelle L. 
Published: December 2019

AbstractPosttraumatic osteoarthritis (PTOA) involves the mechanical and biological deterioration of articular cartilage that occurs following joint injury. PTOA is a growing problem in health care due to the lack of effective therapies combined with an aging population with high activity levels. Recently, acute mitochondrial dysfunction and altered cellular respiration have been associated with cartilage degeneration after injury. This finding is particularly important because recently developed mitoprotective drugs, including SS peptides, can preserve mitochondrial structure and function after acute injury in other tissues. It is not known, however, if cartilage injury induces rapid structural changes in mitochondria, to what degree mitochondrial dysfunction in cartilage depends on the mechanics of injury or the time frame over which such dysfunction develops. Similarly, it is unknown if SS‐peptide treatment can preserve mitochondrial structure and function after cartilage injury. Here, we combined fast camera elastography, longitudinal fluorescence assays, and computer vision techniques to track the fates of thousands of individual cells. Our results show that impact induces mechanically dependent mitochondrial depolarization within a few minutes after injury. Electron microscopy revealed that impact causes rapid structural changes in mitochondria that are related to reduced mitochondrial function, namely, fission and loss of cristae structure. We found that SS‐peptide treatment prior to impact protects the mitochondrial structure and preserves mitochondrial function at levels comparable with that of unimpacted control samples. Overall, this study reveals the vital role of mitochondria in mediating cartilage's peracute (within minutes) response to traumatic injury and demonstrates mitoprotection as a promising therapeutic strategy for injury‐induced cartilage damage.
Oncoplastic breast‐conserving surgery using chest wall perforator flaps: Three‐dimensional quantitative analysis of the percentage of breast volume excised and changes over time in flap volume
Authors: Fujimoto Hiroshi , Shiina Nobumitsu , Nagashima Takeshi , Sangai Takafumi , Sakakibara Junta , Takada Mamoru , Teranaka Ryotaro , Takishima Haduki , Kawasaki Yohei , Ohtsuka Masayuki 
Published: December 2019

AbstractBackground and ObjectivesWe investigated the effects of oncoplastic breast‐conserving surgery (BCS) using chest wall perforator flaps (CWPFs) on the subsequent expected deformity and evaluated the longevity of flap volume.MethodsWe retrospectively reviewed oncological and cosmetic outcomes of 33 women who had undergone the above procedure. We calculated the percentage of breast volume excised (PBVE) from computed tomography volumetry and compared it between a historical BCS alone and the study (flap) group. We also sequentially evaluated flap volumes by magnetic resonance imaging volumetry.ResultsOncoplastic BCS using 25 lateral flaps and eight inferior flaps, depending on the site of the defect, was performed; mean PBVEs were 31.1% and 19.0%, respectively. No local and two distant recurrences occurred in a median follow‐up of 61 months. PBVE was 2.6 times larger in the flap than in the BCS alone group. Over half the patients in the BCS alone group had poor cosmetic results when PBVE exceeded 15%, whereas patients in the flap group achieved good cosmetic results with PBVE >25%. In most patients, 80% of flap volume was maintained 5 years after surgery.ConclusionsCWPF improves cosmetic outcomes in patients with predicted deformity after BCS alone and maintains its volume for at least 5 years.
Factors Affecting Glucocorticoid and Thyroid Hormone Production of Island Foxes
Authors: Kozlowski Corinne P. , Clawitter Helen , Guglielmino Angela , Schamel Juliann , Baker Stacy , Franklin Ashley D. , Powell David , Coonan Timothy J. , Asa Cheryl S. 
Published: December 2019

ABSTRACTThe island fox (Urocyon littoralis) is native to 6 of the 8 Channel Islands of California, USA. The species experienced a population decline in the 1990s but recovered after predatory golden eagles (Aquila chrysaetos) were relocated and feral pigs (Sus scrofa), a main food source for the eagles, were removed. As part of an ongoing conservation program, the National Park Service conducts yearly health surveys on foxes residing on Santa Rosa and San Miguel islands. In this study, we document non‐invasive measures of stress and nutritional status from fecal samples collected during surveys from 2009 to 2015. We collected samples defecated in traps overnight or during handling and measured concentrations of glucocorticoid (GC) and triiodothyronine (T3) metabolites using validated assays. We used generalized linear mixed models to assess the relationships between hormones, season, island, age class, sex, body condition, reproductive status, and ectoparasite presence. Overall, males had marginally lower fecal T3 concentrations than females. Concentrations of both hormones positively correlated with body condition. Fecal GC production varied seasonally; concentrations were highest from December to February and declined through the summer and fall. During summer, younger females and those with signs of recent reproduction had higher fecal GC concentrations than older females or those without evidence of reproduction. Fecal T3 concentrations did not vary in relation to season, age, or reproductive status, but on San Miguel Island were positively correlated with ectoparasite presence. There were no other significant differences between islands. Our results provide hormone data for island foxes and demonstrate that production varies in relation to seasonal and biological factors. These reference data will serve as a comparison for future health surveys and allow managers to identify factors associated with increased stress or reduced nutritional state. © 2019 The Wildlife Society.