bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Drug Rediscovery Protocol (DRUP trial): A Dutch National Study on behalf of the Center for Personalized Cancer Treatment (CPCT) to Facilitate Patient Access to Commercially Available, Targeted Anti-cancer Drugs to determine the Potential Efficacy in Treatment of Advanced Cancers with a Known Molecular Profile
Authors: Voest Emile , Verheul Henk , Gelderblom Hans , Velden Daphne van der , Hoes Louisa , Wijngaart Hanneke van der , Henegouwen Maxime van Berge 
Published: March 2020

Abstract
This is a prospective, non-randomized clinical trial that aims to describe the efficacy and toxicity of commercially available, targeted anticancer drugs* prescribed for treatment of patients with advanced cancer with a potentially actionable variant as revealed by a genomic or protein expression test. The study also aims to simplify patient access to approved targeted therapies that are contributed to the program by collaborating pharmaceutical companies and to perform next generation sequencing on tumor biopsies for biomarker analyses. Eligible patients have an advanced solid tumor, multiple myeloma or non-Hodgkin lymphoma for which standard treatment options are no longer available and acceptable performance status and organ function. A genomic or protein expression test must have been performed on the tumor and the results must identify at least one potentially actionable molecular variant as defined in the protocol. Results from the molecular profiling test will be used to determine an appropriate drug(s) from among those available in the protocol. The choice of drug will be supported by a list of potential profiles, a molecular tumor board, a knowledge library and by study coordinators for review and approval of the match. The protocol-specified treatment will be administered to the patient once any drug-specific eligibility criteria are confirmed and a fresh pre-treatment biopsy is performed for future biomarker studies. All patients who receive treatment with a drug available in the protocol will be followed for standard efficacy outcomes including tumor response, progression-free and overall survival as well as duration of treatment. In addition, treatment related toxicity will be evaluated.
Optogenetic control of mRNA localization and translation in live cells
Authors: Kim Na Yeon , Lee Sangkyu , Heo Won Do 
Published: March 2020

Abstract
Numerous efforts have been made toward the goal of visualizing the spatiotemporal dynamics of single mRNA molecules, yet our capacity for precisely controlling their functions lags behind. Here, we present an optogenetic approach for manipulating the localization and translation of specific mRNAs in live cells. Our technique combines blue light-responsive protein-protein interactions with mRNA visualization modules to robustly and reversibly generate protein clusters that can trap specific mRNA molecules. This sequestration reduces the binding chance of mRNAs with ribosomes, thereby dramatically attenuating protein synthesis
Novel T7-modified pH-responsive targeted nanosystem for co-delivery of docetaxel and curcumin in the treatment of esophageal cancer
Authors: Deng Lian , Zhu Xiongjie , Yu Zhongjian , Li Ying , Qin Lingyu , Liu Zhile , Feng Longbao , Guo Rui , Zheng Yanfang 
Published: March 2020

Abstract
Although single-drug chemotherapy is still an effective treatment for esophageal cancer, its long-term application is limited by severe side effects. Nanomedicines have increasingly attracted attention because of their good biological safety, targeting and high-efficiency loading of multiple drugs. Herein, we have developed a pH-responsive nanocarrier that has high affinity for the transferrin receptor, which is overexpressed by tumor cells. The system is capable of simultaneous delivery of the chemotherapy drug, docetaxel, and the Chinese Medicine, curcumin, for treatment of esophageal cancer. This novel T7-modified targeting nanosystem releases loaded drugs when exposed to the acidic microenvironment of the tumor, and exerts a synergistic anti-tumor effect, and T7-NP-DC with docetaxel and curcumin loading of 10% and 6.1%, respectively. In vitro and in vivo studies showed that improved anti-tumor efficacy could be obtained by loading docetaxel and curcumin into the T7-modified nanocarrierwithout obvious toxicity or side effects, compared to drug without nanocarrier. Furthermore, the nanocarriers conjugated with T7 short peptides were more readily taken up by esophageal cancer cells compared with normal cells.Together, our findings indicate that the materials can safely exert synergistic anti-tumor effects and provide an excellent therapeutic platform for combination therapy of esophageal cancer.
Biomechanical assessment and finite element analysis of a simulated axial-loading experiment on a wrist protector model
Authors: He Jian Ying , Zhang Li 
Published: March 2020

Abstract
Objective

To evaluate the biomechanical analysis and effect of the wrist protector and provide a theoretical basis for wrist fractures and the optimal design of wrist protectors.

Methods

6 cadaveric wrist models were axially loaded 600 N stress, and the stress magnitude and distribution of the experimental group (wearing wrist protectors) and control group were obtained. Furthermore, a three-dimensional finite element analysis was conducted to verify the scientificity and effectiveness of the models.

Results

The stresses on the radial distal palmar, ulnar distal palmar, radial distal dorsal, ulnar distal dorsal, radial proximal palmar and ulnar proximal palmar units in the experimental group were lower than those in the control group (P < 0.05). However, the stresses on the radial proximal dorsal and ulnar proximal dorsal units were higher than those in the control group (P>0.05).

Conclusion

The stress on the radioulnar palmar unit was high, while the radioulnar dorsal unit one was relatively low. Within the range of physiological loads, wearing wrist protectors can significantly reduce the stress on the radioulnar distal palmar, radioulnar proximal palmar and radioulnar distal dorsal units.
“Universal test and treat” program reduced TB incidence by 75% among a cohort of adults taking antiretroviral therapy (ART) in Gurage zone, South Ethiopia
Authors: Girum Tadele , Yasin Fedila , Dessu Samuel , Zeleke Bereket , Geremew Mulugeta 
Published: March 2020

Abstract
Background: Tuberculosis (TB) remains the leading cause of morbidity and mortality in peoples living with HIV. At least twenty five percent of deaths are attributed to TB. It is believed that, Universal test and treat (UTT) program for HIV reduces incidence of TB and most countries implement the program. However, there is no study conducted to evaluate the impact of UTT on TB incidence. Therefore, by recruiting a cohort of ART users in the “UTT” and “differed treatment/CD4 based” programs we aimed to measure the effect of the UTT program on incidence of TB. Objective: To measure the effect of “UTT” program on TB incidence among a cohort of adults taking antiretroviral therapy (ART) in Gurage zone, South Ethiopia. Methods: Health facility based retrospective cohort study through record review of 5 year (2014-2019) cohort was conducted in public facilities of Gurage Zone. Randomly selected 384 records were reviewed by using standardized structured checklist by trained professionals. Data was entered by Epi info version 7 and analyzed by STATA. Generalized Linear Model with binomial link function was fitted to measure adjusted incidence density/Incidence rate ratio and identify predictors of incidence difference between the two programs. Results: During the follow up period, 39 incident TB cases were occurred, and making the overall incidence rate of 4.79/100 person-year. It is significantly lower in the UTT (IR=2.10/100 person-year) than the differed program (IR=6.23/100 person-year). The adjusted Incidence Rate Ratio (AIRR) of TB among patients enrolled in the UTT program was; 0.25 (95% CI=0.08-0.70) compared to patients enrolled in the differed program. Thus, UTT program reduce TB incidence by 75%. In addition to the program, IPT use (AIRR= 0.35 (95% CI=0.22-0.48)), WHO Stage I and II (AIRR=0.70 (95% CI=0.61-0.94)) and higher Base line CD4 count (AIRR=0.96 (95% CI=.94-0.99)) significantly reduce incidence of TB. Whereas, treatment failure increase the incidence (AIRR=5.8 (95% CI=1.93-8.46)). Conclusion: TB incidence was significantly reduced by 75% after UTT. Therefore, intervention to further reduce the incidence has to focus on strengthening UTT program and IPT.
Prevalence and associated factors of depression among people with epilepsy in Mekelle, Tigray, Ethiopia, 2019.
Authors: Seid Jemal , Mebrahtu Kalayu 
Published: March 2020

Abstract
Background - Epilepsy is the world’s most common neurological disorder, affecting approximately 50 million people worldwide and contributed to different psychiatric illness. Depression is one of the most frequent co morbid psychiatric disorders that affects the life of the patients. This comorbidity in people with epilepsy impose significant burdens on patients and their families. The study aimed to determine the prevalence of depression and associated factors among peoples with epilepsy in Mekelle , Ethiopia. Methods – An institutional based cross-sectional study was conducted among systematically selected 296 people with epilepsy from April 15, 2019 – May 30, 2019. Depression was assessed by using the Patient health questionnaire (PHQs). The data were entered & clean by using Epi Info 4.4 version. Statistical Package for Social Sciences version 25 was used for analysis. Factors with a p-value < 0.30 in the bivariate logistic regression were entered into the multivariate model. A p-value of less than 0.05 was considered statistically significant. Result - The prevalence of depression was found to be 34.8%. Unable to read & write (AOR= 0.400, 95% CI: 0.162, 0.986), long duration of the medication intake at least for 11 years (AOR= 3.715, 95% CI: 1.498, 9.212), absence of improvement with medication (AOR= 0.216, 95% CI: 0.101, 0.460), feeling of perceived stigma (AOR= 0.244, 95% CI: 0.129, 0.462), stress symptoms( AOR= 0.452, 95% CI: 0.220, 0.928), were significantly associated with depression. Conclusion – Nowadays depression among peoples with epilepsy is high. Keywords:­­- Comorbidity, Depression, Epilepsy, Outpatient, Mekelle.
The impact of cervical sagittal parameters on axial neck pain in patients with cervical kyphosis
Authors: Li Jia , Zhang Di , Shen Yong 
Published: March 2020

Abstract
Background:Cervical sagittal alignment is closely related with cervical disc degeneration and impacts the spinal function and quality of life, especially for cervical kyphosis (CK). The purpose of this study was to evaluate the influence of cervical sagittal parameters on the axial neck pain (ANP) in patients with CK.Methods: A retrospective analysis was performed of data from 263 patients who underwent to the authors’ department of this institution between January 2012 and December 2018. Radiographic evaluations were performed at authors’ department, including CK types, C2-7 sagittal vertical axis (SVA), thoracic inlet angle (TIA), T1 slope, neck tilt (NT), cranial tilt and cervical tilt. Sagittal alignment of the cervical spine was classified into 2 types: global and regional type. Multivariate logistic regression analysis was performed to evaluate these parameters as the risk factors for ANP.Results: Patients in this study were divided into 2 groups according to ANP. There were 92 patients in ANP group and 171 patients in Non-ANP group. There was no significant difference in age (P=0.196), gender (P=0.516), TIA(P=0.139), NT (P=0.676), CK types (P=0.533), cranial tilt (P=0.332), cervical tilt (P=0.585) and cervical disc degeneration (P=0.695) between the two groups. T1 slope, C2-7 SVA in the ANP group was significantly higher than in the Non-ANP group (P < 0.05). After multivariate logistic regression analysis, C2-7 SVA (P=0.003, OR 2.318, 95% CI 1.373-4.651) and T1 slope (P=0.028, OR 2.563, 95% CI 1.186-4.669) were the two risk factors for the ANP.Conclusions: Based on this study, cervical sagittal parameters have a significant effect on the happening of axial neck pain in patients with CK. A higher T1 slope and larger C2-7 SVA are closely lead to the development of neck pain.
Development and characterization of Protein Kinase B/AKT isoform-specific Nanobodies
Authors: Merckaert Tijs , Zwaenepoel Olivier , Gevaert Kris , Gettemans Jan 
Published: March 2020

Abstract
Background The serine/threonine protein kinase AKT is frequently over-activated in cancer and is associated with poor prognosis. As a central node in the PI3K/AKT/mTOR pathway, which regulates various processes considered to be hallmarks of cancer, this kinase has become a prime target for cancer therapy. However, AKT has proven to be a highly complex target as it comes in three isoforms (AKT1, AKT2 and AKT3) which are highly homologous, yet non-redundant. The isoform-specific functions of the AKT kinases can be dependent on context (i.e. different types of cancer) and even opposed to one another. To date, there is no isoform-specific inhibitor available and no alternative to genetic approaches to study the function of a single AKT isoform. Results We have developed and characterized nanobodies that specifically interact with the AKT1 or AKT2 isoforms. These new tools should enable future studies of AKT1 and AKT2 isoform-specific functions. Furthermore, for both isoforms we obtained a nanobody that interferes with the AKT-PIP3-interaction, an essential step in the activation of the kinase. Conclusions The nanobodies characterized in this study, which can be expressed in mammalian cells, are a new stepping stone towards unravelling AKT isoform-specific signalling and can lead to the first isoform-specific AKT inhibitor.
Focal Maculopathy in the Senile Cataract Population
Authors: zhang mei , Yuan Kun , Liu Jingwen , Zheng Haoran 
Published: March 2020

Abstract
Objective Optical Coherence tomography (OCT) was employed to screen for maculopathy in the senile cataract population, investigate its incidence, and establish a healthy mode of management for maculopathy. Methods A visual examination, slit-lamp microscope examination, direct ophthalmoscopic fundus examination, and a macular OCT examination were performed on 102 people with senile cataracts who were over 60 years in our hospital from January 1, 2019, to July 31, 2019. The demographic characteristics such as sex, age, physical examination mode: organization/individual, and routine physical examination items: presence or absence of hypertension, body mass index (BMI), blood biochemistry, total cholesterol, triglyceride (TG), fasting blood glucose and others. Results Of the 102 subjects in the study, 28 were positive for maculopathy according to the OCT examination. Univariate analysis found that there were statistical differences between sexes and the presence of maculopathy (p<0.05). There were no significant differences in age, visual acuity, hypertension, BMI, fasting blood glucose, TG, total cholesterol, and cataract type ( P >0.05). Multivariate logistic regression analysis, including all factors that might affect maculopathy, indicated that positivity for maculopathy and age (OR =2.549, 95%CI: 1.129–5.756, p<0.05) and gender (OR=3.907, 95%CI: 1.241–12.302, p<0.05) were related. Conclusion The incidence of maculopathy in the senile cataract population was higher than that in the elderly population without cataract disease, and the proportion is 27.45%. Screening for maculopathy in the senile cataract population, especially among the elderly and females, should be improved.
Nosocomial Tuberculosis Transmission from 2006 to 2018 in Hospital, China
Authors: XIE ZHONGYAO , ZHOU NING , CHI YUQING , HUANG GUOFANG , WANG JINGPING , GAO HUI , XIE NA , MA QIANHUI , YANG NAN , Nie Wenjuan , CHU NAIHUI 
Published: March 2020

Abstract
Background: Strong evidence is lacking to support effectiveness of currently implemented tuberculosis infection prevention control (TB-IPC) measures for preventing nosocomial tuberculosis (TB) transmission. This 13-year analysis is the longest follow-up investigation to date to identify risk factors underlying nosocomial TB transmission. Methods: We monitored all staff of Beijing Chest Hospital each year from 2006 to 2018. Age, gender, duration, department, education, income, respirator, ultraviolet, and ventilation were chosen as variables. Univariate cox regression, correlation analysis, and multivariate cox regression were analyzed sequentially.Results: Using multivariable cox regression analysis, variables of income, ultraviolet germicidal irradiation (UVGI), natural ventilation and mechanical ventilation conferred significant protective effects, with odds ratios (ORs) of 0.499, 0.058, 0.003, and 0.015, respectively (P<0.05). Medical N95 respirator conferred an excellent protective effect, with an associated TB infection rate of 0%. Notably, inadequately maintained mechanical ventilation systems were less protective than natural ventilation systems.Conclusions: UVGI, adequate ventilation, and medical N95 respirator use may be protective factors against nosocomial TB transmission. Adequate ventilator system training, monitoring, and regular maintenance are critical for achieving effective TB-IPC.Corresponding author equally to this paper: Naihui Chu: Tuberculosis Department, Beijing Chest Hospital affiliated to Capital Medical University, No 9, Beiguan street, Tongzhou District, Beijing 101149, P.R.China. Email: chunaihui1994@sina.com
Effects of supplemental β-carotene on growth performance, immune function and intestinal mucin in weaned piglets
Authors: Lang Wuying , Gong Haizhou , Li Ruonan , Wu Min , Chu Qianran , Wang Taiping , Zheng Xin 
Published: March 2020

Abstract
Background Weaning stress causes intestinal immune system disorders. β-carotene displays anti-inflammatory,which can prevent the development of inflammatory diseases. The aim of this study was to determine the effect of supplemental β-carotene on growth performance, immune function and intestinal mucin in weaned piglets.Results A total of 45 piglets with the average body weight of 4.62 ± 0.06 kg (14 d of age) were randomly assigned to 3 treatments with 5 replicate pens per treatment and 3 pigs per pen and weaned at 21 d. The control group (CG) fed the basal diets from 14 d to 24 d of age. The low-dose group (LG) and the high-dose group (HG) fed the basal diets supplemented with 40 or 80 mg/kg β-carotene from 14 d to 24 d of age, respectively. Compared with the CG, t he average daily gain (ADG) and average daily feed intake (ADFI) of piglets in the HG were increased (p<0.05). And the IgA and IL-10 levels in serum in the HG were also higher than those in the CG (p<0.05). The IgM, TNF-α and IL-6 levels in serum in the β-carotene groups were lower than those in the CG (p<0.05). Periodic Acid-Schiff stain results showed that the content of mucopolysaccharide and the number of goblet cells in the intestine in the β-carotene groups were more than those in the CG (p<0.05). The results of immunofluorescence showed that the expression of MUC1 in the intestine in the β-carotene groups was lower than that in the CG (p<0.05). And the expression of mucin1 mRNA in the intestine in the β-carotene groups was also lower than that in the CG (p<0.05). However, compared with the CG, the expression of mucin2 mRNA in the intestine in the HG was increased (p<0.05).Conclusion Supplemental β-carotene could improve growth performance, immune function and intestinal mucin in weaned piglets. It is suspected that our pre-protection of β-carotene in weaned piglets may help weaned piglets safely through the weaning period.
Associations of Aminotransferases with Adverse Outcomes after Acute Ischemic Stroke: Results from China National Stroke Registry
Authors: Zong Lixia , Wang Xianwei , Li Zixiao , Zhao Xingquan , Liu Liping , Li Hao , Meng Xia , Wang Yilong , Wang Yong-Jun 
Published: March 2020

Abstract
Background The relationship between aminotransferases and cardiovascular outcomes has been inconsistent in previous studies. We aimed to investigate the association of aminotransferases with clinical outcomes after acute ischemic stroke (AIS) or transient ischemic attack (TIA). Methods 17,178 AIS or TIA patients with serum alanine aminotransferase (ALT) levels < 120 U/L were included from the China National Stroke Registry (CNSR) for current analysis. Composite endpoint is comprised of recurrent stroke and all-cause mortality. Poor functional outcome is defined as modified Rankin scale of 3-6. Multivariable logistic regression was used to evaluate the risks of one-year all-cause mortality, recurrent stroke, composite endpoint and poor functional outcome according to increasing sex-specific quintiles of ALT/ aspartate aminotransferase (AST) respectively. Results One-year incidences of all-cause mortality, recurrent stroke, composite endpoint and poor functional outcome were 11.9%, 6.0%, 13.7% and 28.2% respectively in patients with the lowest quintile of ALT, and 7.4%, 3.6%, 9.0% and 17.9% respectively in the highest quintile. Compared with the lowest ALT quintile, the adjusted odds ratios with 95% confidence interval of the highest quintile were 0.55 (0.43-0.70) for all-cause mortality, 0.61 (0.45-0.83) for stroke recurrence, 0.62 (0.49-0.77) for composite endpoint, and 0.67 (0.56-0.80) for poor functional outcome. There was no significant interaction of ALT with age, sex, diabetes, dyslipidemia and alcohol consumption for all outcomes (p for interaction ≥ 0.10). Conclusions Low serum ALT may serve as an independent predictor for all-cause mortality, stroke recurrence, composite endpoint and poor functional outcome after stroke.
Association between sitting time and non-alcoholic fatty liver disease in South Korean population: a cross-sectional study: a cross-sectional study
Authors: Joo Jae Hong , Kim Hwi Jun , Park Eun-Cheol , Jang Sung-In 
Published: March 2020

Abstract
Background: To examine the association between sitting time and non-alcoholic fatty liver disease among South Koreans aged ≥20 years. Methods : Data from the 2016–2018 Korea National Health and Nutrition Examination Survey were used for the analysis. Non-alcoholic fatty liver disease was diagnosed according to a hepatic steatosis index of >36. Sitting time was categorized into as < 5, 5 -7, 8 – 10, and > 10 hours. Multiple logistic regression analysis was used to examine the association between sitting time and non-alcoholic fatty liver disease in South Korean population. Results : A total of 13,518 participants were enrolled. The odds for having NAFLD in 5 – 7, 8 – 10, and > 10 sitting hours were 1.04 (CI: 0.85-1.28), 1.23(CI: 1.01-1.51), and 1.33(CI: 1.10-1.62), respectively. The odds ratio increased in magnitude with longer hours of sitting time (test for trend: P-value = 0.0009). Conclusion : Advising physical exercises and discouraging sedentary activities may help to alleviate NAFLD among the South Korean population.