bluuebunny/crossref_metadata_2025_split huggingface dataset:
Tracking the Features of CIMP-positive Glioma: A Systematic Review and Meta-Analysis
Authors: Xiao Huashi , Xu Yingying , Shen He-chun , Liu Wanjun , Tan Siyuan , Chen Ping , Yu Guo , Ren Chuanli , Sun Liangdan , Yu Duonan , Zong Liang 
Published: March 2020

Abstract
Backgrounds Controversy surrounding CpG island methylator phenotype (CIMP) in glioma exists with regard to its prognostic value.Methods PubMed, EMBASE, and Cochrane Library databases were searched for studies describing molecular and clinicopathological features, and overall survival of gliomas stratified by CIMP status. Associations of CIMP with outcome parameters were estimated using odds ratio (OR) or hazard ratios (HRs) with a 95% confidence interval (CI) using a fixed or random effects model.Results A total of 12 studies involving 2386 gliomas (1051 CIMP-positive and 1335 CIMP-negative) were included. Molecular analysis showed that CIMP is more frequent in IDH1-mutated gliomas (OR 229.07; 95% CI 138.72–378.26) and 1p19q LOH gliomas (OR 5.65; 95% CI 2.66–12.01), though CIMP was not associated with EGFR mutation (OR 0.14; 95% CI 0.05–0.43) or MGMT promoter methylation (OR 3.01; 95% CI 0.79–11.48). Clinicopathological analysis showed that CIMP is more frequent in oligodendroglioma (OR 5.51; 95% CI 3.95–7.70), but less frequent in glioblastoma (OR 0.14; 95% CI 0.10–0.19). However, CIMP was not associated with anaplastic oligoastrocytomas (OR 1.57; 95% CI 1.24-2.00) or oligoastrocytoma (OR 0.79; 95% CI 0.35–1.76). We found that CIMP-positive glioma was associated with a longer overall survival (HR -0.57; 95% CI -0.97– -0.16).Conclusions A CIMP-positive glioma has better prognosis and its own molecular features (such as IDH1 and 1p19q LOH mutations) and clinicopathological features. These conditions suggest CIMP could be used as an independent prognostic marker for glioma.
Intratumoral cycling hypoxia inhibits expression level of estrogen receptor to promote the formation of heterogeneous sub-clones in luminal A breast cancer
Authors: Song Bing , Zhang Gaofeng , Li Jianyi , Kang Ye , Kong Linna , Ma Yan , Shu Hong , Zhang Yang , Jia Shi , Wang Zhenrong 
Published: March 2020

Abstract
Background The expression level of estrogen receptor (ER) is positively correlated with chemoresistance in patients with Luminal A tumor (LAT). ER expression level alters frequently after neoadjuvant chemotherapy which may be related with hypoxia. The spatial and temporal heterogeneity of tumor sub-clones is one of the major contributors to treatment failure. It is essential to investigate how the intratumoral heterogeneous sub-clones survive hypoxic microenvironment in LAT. Material and Methods LAT with largest cross section was divided into micro-sections, the expressions of hypoxia inducible factor-1 (HIF-1) and ER were then detected by immunohistochemistry. The intratumoral distribution of micro-vessels was assessed by 3D reconstruction and stained CD34; the cycling hypoxia model of MCF-7 was established in a step fashion to investigate the changes in HIF-1 and ER expressions. All statistical analyses were performed using SPSS software (version 17.0 for Windows). Results There was a negative correlation between the expressions of ER-alpha and HIF-1alpha (c=-2.40; p=0.044) as assessed by mean optical density values in the maximal cross section of tumor. As shown by 3D ultrasound image, the center of tumor had less functional micro-vessels compared with the periphery (P<0.05). From the periphery to the center of tumors in the large sections from 8 patients with LAT, the expressions of ER, progesterone receptor and CD34 were gradually decreased with HIF-1alpha expression showing an opposite direction. There was a negative correlation between expressions of ER-alpha and HIF-1alpha, and between expressions of CD34 and HIF-1alpha. No significant difference was noted in the outcomes of cytometry between the hypoxia and control groups. ER-alpha expression gradually decreased with the time course of cycling hypoxia with HIF-1alpha showing an opposite direction. Conclusion LAT is composed of heterogeneous sub-clone cells which can be distinguished by ER-alpha and HIF-1alpha. The distribution of heterogeneous sub-clone cells was closely related with heterogeneous microenvironment of hypoxia / reoxygenation. Keywords: Luminal A breast cancer, cycling hypoxia, estrogen receptor, heterogeneous sub-clones.
Verteporfin Inhibits Cell Proliferation and Induces Apoptosis in Different Subtypes of Breast Cancer Cell Lines Without Light Activation
Authors: Wei Changran , Li Xiangqi 
Published: March 2020

Abstract
Background

Breast cancer (BC) can be separated into four molecular subclassifications including Lumina1 A, Lumina1 B, HER-2 overexpression and Basal-like subtype. These classifications are based on estrogen receptor (ER), progesterone receptor (PR), human epidermal growth factor receptor-2 (HER-2) and cell proliferation antigen (Ki-67). The Hippo signaling pathway plays an indispensable role in BC. The YAP1 gene is a terminal effector of Hippo pathway, and hyperactivation of YAP mediates tumorigenesis. As an inhibitor of YAP, non-photoactivated verteporfin (VP) can inhibit YAP-mediated tumor proliferation and angiogenesis by eliminating its interaction with TEAD. This study set out to determine the effect and molecular mechanisms of VP-mediated inhibition of YAP in different subtypes of BC.

Methods

Luminal A, Luminal B and Basal-like BC cells were cultivated in vitro in order to study effect of VP on proliferation and apoptosis on these three molecular BC subtypes.

Results

Our experimental results show that VP inhibits cell proliferation, YAP-TEAD interaction and its downstream target expression. VP also induces tumor cell apoptosis, and promotes the cleavage of Caspase-9 and PARP in various molecular subtypes of BC cells.

Conclusion

These findings provide a basis for VP as a potential anti-tumor therapeutic for BC by targeting the Hippo pathway effector YAP.
HIV understanding, experiences and perceptions of HIV-positive men who have sex with men in Amazonian Peru: A qualitative study.
Authors: Tattsbridge Jasmine , Wiskin Connie , De Wildt Gilles , Llavall Anna Clavé , Ramal-Asayag César 
Published: March 2020

Abstract
Background HIV-related incidence and mortality is increasing across Peru, with highest mortality rates recorded in Amazonian Loreto. This epidemic is concentrated in MSM, a population with 14% HIV treatment adherence despite free national provision. This study investigates barriers and facilitators to following healthcare advice through experiences and perceptions of HIV-positive MSM and healthcare professionals in Loreto. Methods Twenty qualitative interviews with HIV-positive MSM and one focus group with HIV-specialist healthcare professionals were conducted in Loreto, January-February 2019. Thematic content analysis was used. Results A culture of isolation and discrimination was identified, propagated by poor public knowledge surrounding HIV transmission and treatment. Employment potential was hampered and 7/20 patients had suicidal thoughts post-diagnosis. Barriers to care included: shame, depression, travel cost/times, a preference for traditional plant-based medicine and side-effects of ART. Facilitators included: education, family and clinic support, disease acceptance and lifestyle changes.Conclusion More targeted community education and workplace discrimination investigations are recommended.
Measuring Character Strengths as Possible Protective Factors against Suicidal Ideation in Older Chinese Adults: A Cross-Sectional Study
Authors: Cheng Xinfeng , Bu He , Duan Wenjie , He Along , Zhang Yaping 
Published: March 2020

Abstract
Background: Suicide is a global issue among the elderly. The number of older people committing suicide is proliferating, and the elderly suicide rate is the highest among all age groups in China. The possible protective factors against suicidal ideation should be investigated for prevention and intervention efforts. The objectives of the present study are as follows: (1) to examine the psychometric properties of the three-dimensional inventory of character strengths (TICS) with a sample of older adults; (2) to investigate correlations among suicide ideation, wellbeing, and character strengths; and (3) to explore the possible protective roles of the three character strengths and wellbeing in explaining suicidal ideation among older adults. Methods: A cross-sectional study comprising 308 older adults aged at least 50 years old from nursing homes was conducted. Four questionnaires, namely, the TICS, the Geriatric Suicide Ideation Scale—10 items, the Brief Inventory of Thriving, and the Satisfaction with Life Scale, were used. Exploratory structural equation modeling, intraclass correlation coefficients, partial correlations, and sets of hierarchical regressions were adopted to estimate and report the results. Results: TICS could be used to assess the character strengths (i.e., caring, inquisitiveness, and self-control) among older adults with an acceptable goodness-of-fit (chi square = 157.30, df = 63, p < 0.001, CFI = 0.94, TLI = 0.90, RMSEA = 0.07, 90% CI = [0.06, 0.08]). Wellbeing (life satisfaction: B = −0.14, t = −2.55, p = 0.01; thriving: B = −0.52, t = −9.64, p < 0.001) and character strengths (caring: B = −0.45, t = −11.84, p < 0.001; inquisitiveness: B = −0.33, t = −7.89, p < 0.001; self-control: B = −0.45, t = − 10.83, p < 0.001) have exhibited a significant association with suicidal ideation among older adults. Moreover, character strengths have shown an independently cross-sectional relationship with suicidal ideation, explaining 65.2% of the variance of suicidal ideation after controlling for the wellbeing and demographics. Conclusion: This study indicated that character strengths were associated with low levels of suicidal ideation. Therefore, the protective factors against suicidal ideation among older adults should be given additional attention.
Rapid development of a synthetic DNA vaccine for COVID-19
Authors: Smith Trevor R.F. , Patel Ami , Ramos Stephanie , Elwood Dustin , Zhu Xizhou , Yan Jian , Yang Maria , Chokkalingam Neethu , Gary Ebony N. , Schultheis Katherine , Pezzoli Patrick , Walters Jewell , Walker Susanne N. , Parzych Elizabeth , Reuschel Emma L. , Doan Arthur , Tursi Nicholas , Vasquez Miguel , Choi Jihae , Maricic Igor , Bah Mamadou A. , Wu Yuanhan , Amante Dinah , Park Daniel , Dia Yaya , Ali Ali Raza , Zaidi Faraz I. , Generotti Alison , Kim Kevin Y. , Herring Timothy A. , Reeder Sophia , Brown Ami Shah , Khoshnejad Makan , Wang Nianshuang , Chu Jacqueline , Wrapp Daniel , McLellan Jason S , Muthumani Kar , Kim J Joseph , Boyer Jean , Kulp Daniel W. , Humeau Laurent M.P.F. , Weiner David B. , Broderick Kate E. 
Published: March 2020

Abstract
The coronavirus family member, SARS-CoV-2 has been identified as the causal agent for the outbreak of viral pneumonia disease, COVID-19 which first emerged in mid-December 2019 in the city of Wuhan in central China. As of February 25, 2020 there are 80,994 people infected and 2,760 deaths, and documented human-to-human transmission across multiple continents. At this time, no vaccine is available to control further dissemination of the disease. We have previously developed a synthetic DNA vaccine targeting the MERS coronavirus Spike (S) protein that was deployed in response to the MERS outbreak in South Korea. This vaccine induced potent antibody and CTL responses, and provided protection in a NHP challenge model. In the clinic, the vaccine generated humoral immunity including neutralizing antibody responses, as well as T cell immunity. Here we build on this prior work and report on the rapid development of a synthetic DNA-based vaccine targeting the major surface antigen Spike protein of SARS-CoV-2. The engineered construct, INO-4800 induced robust expression of the Spike protein in vitro, and generated antibody and T cell responses following a single immunization in mice and guinea pigs. This preliminary dataset identifies INO-4800 as a potential COVID-19 vaccine candidate, supporting further study for mobilization against this emerging disease threat.
Effects of Probiotics on Diabetic Nephropathy: A Systematic Review
Authors: Vlachou Eugenia , Ntikoudi Anastasia , Govina Ourania , Lavdaniti Maria , Kotsalas Nikolaos , Tsartsalis Athanasios , Dimitriadis George 
Published: March 2020

Background:
Diabetic Nephropathy is a frequent complication of diabetes mellitus due
to functional and structural modifications in multiple kidney compartments. Probiotics have risen
lately as a forthcoming therapeutic intervention but they have not been systematically evaluated in
diabetic nephropathy so far. The aim of this systematic review was to evaluate randomized controlled
trials and experimental studies assessing the effect of probiotic supplements on diabetic
nephropathy.

Methods:
An extensive literature search was conducted through electronic databases (PubMed,
Scopus, Cinahl and Medline) with the Medical Subject Headings and entry terms of “diabetic nephropathy”,
“diabetic renal disease” and “probiotics”. The search yielded 116 results, 9 of which met
the inclusion criteria for this systematic review.

Results:
Most of the microorganisms used in the studies belonged to the Lactobacillus and Bifidobacterium
genus. The dosage ranged from 2×107 to 6×1010 CFU/ g. The form of the probiotics
varied across the studies (capsules, sachets, soy milk, kefir and honey). The majority of the studies
demonstrated the benefits of probiotic supplementation on the reduction of inflammation, oxidative
stress and on the amelioration of renal function biomarkers in subjects with diabetic nephropathy. No
major gastrointestinal adverse events were observed during the intervention time with probiotics.

Conclusion:
: Findings of this systematic review demonstrate the positive impact of probiotics on
Diabetic Nephropathy without any major adverse events. Moreover, future larger randomized controlled
trials with bigger samples and longer follow-up time are deemed necessary for further valid
results on the effectiveness of probiotic supplementation on Diabetic Nephropathy.
Characterization of a New Positive Allosteric Modulator of AMPA Receptors - PAM-43: Specific Binding of the Ligand and its Ability to Potentiate AMPAR Currents
Authors: Vyunova Tatiana V. , Andreeva Lioudmila A. , Shevchenko Konstantin V. , Grigoriev Vladimir V. , Palyulin Vladimir A. , Lavrov Mstislav I. , Bondarenko Ekaterina V. , Kalashnikova Elena E. , Myasoedov Nikolay F. 
Published: March 2020

Background:
Currently, the most dynamic areas in the glutamate receptor system neurobiology
are the identification and development of positive allosteric modulators (PAMs) of glutamate
ionotropic receptors. PAM-based drugs are of great interest as promising candidates for the treatment of
neurological diseases, such as epilepsy, Alzheimer's disease, schizophrenia, etc. Understanding the
molecular mechanisms underlying the biological action of natural and synthetic PAMs is a key point for
modifying the original chemical compounds as well as for new drug design.

Objective:
We are trying to elaborate a system of molecular functional screening of ionotropic glutamate
receptor probable PAMs.

Methods:
The system will be based on the radioligand - receptor method of analysis and will allow
rapid quantification of new AMPAR probable PAMs molecular activity. We plan to use a tritiumlabeled
analogue of recently elaborated ionotropic GluR probable PAM ([3H]PAM-43) as the main
radioligand.

Results:
Here, we characterized the specific binding of the ligand and its ability to potentiate ionotropic
GluR currents. The existence of at least two different sites of [3H]PAM-43 specific binding has been
shown. One of the above sites is glutamate-dependent and is characterized by higher affinity. “Patchclamp”
technique showed the ability of PAM-43 to potentiate ionotropic GluR currents in rat cerebellar
Purkinje neurons in a concentration-dependent manner.

Conclusion:
The possibility of using PAM-43 as a model compound to study different allosteric effects
of potential regulatory drugs (AMPAR allosteric regulators) was shown. [3H]PAM-43 based screening
system will allow rapid selection of new AMPAR probable PAM structures and quantification of their
molecular activity.
Los Derechos Humanos en la jurisdicción española
Authors: Carrillo Marc 
Published: March 2020

<p>La posición de los derechos humanos en la jurisdicción española está integrada en el sistema europeo del constitucionalismo multinivel. La jurisdicción ordinaria es la sede habitual de protección de los derechos mientras que es una garantía excepcional. La regla interpretativa del margen de apreciación nacional que caracteriza a la justicia convencional de la Corte Europea de Derechos Humanos no puede ser utilizada para evitar los casos conflictivos. Por otra parte, el uso habitual procedimiento de la cuestión prejudicial ante el Tribunal de Luxemburgo ha provocado la descentralización del sistema de garantías de los derechos humamos en Europa. </p>
IMPLEMENTASI PEMBELAJARAN DAN PENILAIAN HOTS PADA SISWA KELAS 4 SEKOLAH INDONESIA BANGKOK THAILAND
Authors: Yayuk Erna , Deviana Tyas , Sulistyani Nawang 
Published: March 2020

ABSTRAK Implementasi kurikulum 2013 menuntut guru untuk melakukan penilaian HOTS. Pada dasarnya penilaian HOTS harus diawali dengan pembelajaran yang HOTS juga. Hasil yang didapatkan berdasarkan pengamatan di SIB Thailand bawasannya masih ditemukan adanya (1) beberapa guru yang masih memerlukan updating tentang pengetahuan terutama kurikulum 2013, (2) kurang lebih 45% siswa masih pasif ketika diajar dan keaktifan kelas sering didominasi oleh anak-anak yang pintar. (3) dalam proses pembelajaran soal-soal yang dibuat guru kebanyakan masih pada level C1-C3, dan (4) kemampuan siswa dalam berfikir kritis dan analitis sangat kurang. Berdasarkan analisis masalah tersebut, tujuan penelitian ini untuk meningkatkan kemampuan guru-guru terhadap pembelajaran dan penilaian HOTS. Metode penelitian yang digunakan yaitu penelitian kualitatif. Adapun hasil yang didapatkan meliputi (a) hasil contoh perangkat pembelajaran dan penilaian HOTS yang meliputi RPP, (b) Lembar Kegiatan Siswa (LKS), (c) evaluasi pembelajaran, (d) instrumen penilaian HOTS, dan (e) video pembelajaran dan penilaian HOTS. Kata kunci: Pembelajaran, Penilaian, HOTS ABSTRACTThe implementation of the 2013 curriculum requires teachers to conduct HOTS assessments. Basically the HOTS assessment must begin with HOTS learning as well. The results obtained based on observations at SIB Thailand are still found in (1) some teachers who still need updating of knowledge, especially the 2013 curriculum, (2) approximately 45% of students are still passive when taught and class activeness is often dominated by smart children. (3) in the learning process the questions made by the teacher are mostly still at levels C1-C3, and (4) the ability of students in critical and analytical thinking is very lacking. Based on the analysis of the problem, the purpose of this study was to improve the ability of teachers to learn and assess HOTS. The research method used is qualitative research. The results obtained include (a) the results of examples of HOTS learning and assessment devices which include lesson plans, (b) Student Activity Sheets (LKS), (c) learning evaluation, (d) HOTS assessment instruments, and (e) videos learning and assessment HOTS. Key words: Learning, Assessment, HOTS
PERANCANGAN TRAINER MIKROKONTROLER SEBAGAI MEDIA PEMBELAJARAN UNTUK MENINGKATKAN NILAI PEREKAYASAAN SISTEM KONTROL PADA SMK
Authors: Suryania Febriana , Sukardjo Moch. , Yusro M. 
Published: March 2020

Penelitian menggunakan R&D dengan metode ADDIE dan Borg & Gall sebagai penelitian pendahuluan. Penelitian dilakukan di SMK Teknik Elektronika Industri, dengan jumlah responden 31 siswa yang akan mengambil mata pelajaran perekayasaan sistem kontrol. Penelitian ini merancang trainer mikrokontroler sebagai media pembelajaran pada kurikulum 2013 dengan melihat nilai kognitif dan psikomotor sebagai hasil peningkatan siswa. Penelitian ini dibuat karena perubahan kurikulum yang berdampak pada perubahan mata pelajaran praktikum yang mengakibatkan kebutuhan akan prasarana sebagai penunjang belajar praktikum. Penilaian menggunakan tes pilihan ganda yang sudah divalidasi terlebih dahulu dan kemudian diujikan kepada siswa dengan membandingkan antara nilai pretest dan posttest. Kemudian, penelitian ini menggunakan uji-t sebagai perhitungan dalam mengetahui tingkat efektifitas belajar baik secara kognitif maupun psikomotor.Kata kunci: ADDIE, Trainer Mikrokontroler, psikomotorik, efektifitas
Pengaruh Financial Leverage Terhadap Perataan Laba Dimoderasi Firm Size Di Perbankan Indonesia
Authors: Devi Heidy Paramitha 
Published: March 2020

One of the steps that can be taken to reduce earnings fluctuations is income smoothing, by moving high income in a certain year into an unfavorable period, this is done to create a stable company profit so that it is reflected in good company performance in the eyes of investors. The purpose of this study is to determine whether company size is able to moderate the effect of financial leverage on income smoothing. This research was conducted at companies listed in the 2013-2017 banking period. The number of samples in this study were 110 samples that were selected using the purposive sampling method. The data analysis technique used in this study is logistic regression analysis. The results of this study indicate that financial leverage has no effect on income smoothing, and company size is not able to moderate the effect of financial leverage on income smoothing.
Keywords: Income Smoothing, Financial leverage, Company Size, Banking Sector
JEL Classification:G21, G32, L25, M41
Pengaruh pemberian zat retardan terhadap pertumbuhan dan hasil ubi pada dua kultivar kentang (Solanum tuberosum L.) di dataran medium
Authors: Sumadi Sumadi , Suminar Erni , Murgayanti Murgayanti , Nuraini Anne 
Published: March 2020

Tujuan dari kegiatan penelitian ini adalah untuk mendapatkan informasi mengenai kulti-var kentang dan jenis serta konsentrasi zat retardan yang dapat mendukung pertumbuhan dan perkembangan serta kuantitas dan kualitas hasil ubi bibit kentang di dataran medium. Metode penelitian yang digunakan adalah Rancangan Petak terpisah. Perlakuan terdiri dari petak utama yaitu kultivar yaitu kultivar Nadia dan Granola, dan anak petak yaitu jenis dan konsentrasi zat retardan dengan penggunaan paklobutrazol ( 0; 40; 120 mg L-1 ) atau coumarin (45; 90; 135 mg L-1). Hasil percobaan menun-jukkan bahwa jenis kultivar dan zat retardan berpengaruh terhadap pertumbuhan dan per-kembangan bibit kentang. Terjadi interaksi pada peubah bobot ubi per tanaman, sedangkan peubah tinggi tanaman, kandungan klorofil, jumlah ubi per tanaman, bobot ubi per petak dan persentase jumlah ubi kentang per tanaman berdasarkan pengkelasan ubi bibit tidak terjadi interaksi antara kultivar dan zat retardan. Penggunaan zat retardan pada kultivar Nadia menghasilkan bobot ubi per tanaman lebih tinggi daripada kultivar Granola sedangkan untuk kelas ubi ukuran S dan M dihasilkan lebih tinggi pada kultivar Granola daripada kultivar Nadia. Kata kunci : Kentang ∙ Ubi ∙ Dataran medium ∙ Zat retardan