bluuebunny/crossref_metadata_2025_split huggingface dataset:
A low complexity heuristic to solve a learning objects recommendation problem
Authors: Falci Samuel Henrique , Dorça Fabiano Azevedo , Andrade Alessandro Vivas , Mourão Falci Daniel Henrique 
Published: September 2020

AbstractThe recommendation of learning objects in virtual learning environments has become the focus of research to improve online learning experience. Several approaches have been presented in an attempt to model the individual characteristics of the students and offer learning objects that best suit their particularities. Most of them, though, are impractical in real-world scenarios due to the high computational cost as a huge number of repositories offering learning objects such as Youtube, Wikipedia, Stackoverflow, Github, discussion forums, social networks and many others are available and each has a large amount of learning objects that can be retrieved. In this work, we propose a low complexity heuristic to solve this problem, comparing it to a classical mixed-integer linear programming model and classical genetic algorithm in varying dataset sizes that contain from 2000 to 1360000 learning objects. Performance and optimality were analyzed. The results showed that the proposed technique was only slightly suboptimal, while its computational cost was considerably smaller than the one presented by the linear optimization approach.
Spatiotemporal behavior of a large-scale landslide at Mt. Onnebetsu-dake, Japan, detected by three L-band SAR satellites
Authors: Takada Youichiro , Motono George 
Published: September 2020

AbstractWe applied differential InSAR analysis to the Shiretoko Peninsula, northeastern Hokkaido, Japan. All the interferograms of long temporal baseline (~ 3 years) processed from SAR data of three L-band satellites (JERS-1, ALOS, ALOS-2) commonly indicate remarkable phase changes due to the landslide movement at the southeastern flank of Mt. Onnebetsu-dake, a Quaternary stratovolcano. The area of interferometric phase change matches to known landslide morphologies. Judging from the timing of the SAR image acquisitions, this landslide has been moving at least from 1993 to the present. Successive interferograms of 1-year temporal baseline indicate the temporal fluctuation of the landslide velocity. Especially for the descending interferograms, the positive line-of-sight (LOS) length change, which indicates large subsidence relative to the horizontal movement, is observed in the upslope section of the landslide during 1993–1998, while the negative LOS change is observed in the middle and the downslope section after 2007 indicating less subsidence. The landslide activity culminates from 2014 to 2017: the eastward and the vertical displacement rates reach ~ 6 and ~ 2 cm/yr, respectively. Utilizing high spatial resolution of ALOS and ALOS-2 data, we investigated velocity distribution inside the landslide. During 2007–2010, the eastward component of surface displacement increases toward the east, implying that the landslide extends toward the east. During 2014–2017, the vertical displacement profile exhibits spatially periodic uplift and subsidence consistent with surface gradient, which indicates the ongoing deformation driven by gravitational force. Heavy rainfall associated with three typhoons in August 2016 might have brought about an increase in the landslide velocity, possibly due to elevated pore-fluid pressure within and/or at the base of the landslide material. Also, annual rainfall would be an important factor that prescribes the landslide velocity averaged over 3 years.
A mock circulation loop to test extracorporeal CO2 elimination setups
Authors: Schwärzel Leonie S. , Jungmann Anna M. , Schmoll Nicole , Seiler Frederik , Muellenbach Ralf M. , Schenk Joachim , Dinh Quoc Thai , Bals Robert , Lepper Philipp M. , Omlor Albert J. 
Published: September 2020

AbstractBackgroundExtracorporeal carbon dioxide removal (ECCO2R) is a promising yet limited researched therapy for hypercapnic respiratory failure in acute respiratory distress syndrome and exacerbated chronic obstructive pulmonary disease. Herein, we describe a new mock circuit that enables experimental ECCO2R research without animal models. In a second step, we use this model to investigate three experimental scenarios of ECCO2R: (I) the influence of hemoglobin concentration on CO2removal. (II) a potentially portable ECCO2R that uses air instead of oxygen, (III) a low-flow ECCO2R that achieves effective CO2clearance by recirculation and acidification of the limited blood volume of a small dual lumen cannula (such as a dialysis catheter).ResultsWith the presented ECCO2R mock, CO2removal rates comparable to previous studies were obtained. The mock works with either fresh porcine blood or diluted expired human packed red blood cells. However, fresh porcine blood was preferred because of better handling and availability. In the second step of this work, hemoglobin concentration was identified as an important factor for CO2removal. In the second scenario, an air-driven ECCO2R setup showed only a slightly lower CO2wash-out than the same setup with pure oxygen as sweep gas. In the last scenario, the low-flow ECCO2R, the blood flow at the test membrane lung was successfully raised with a recirculation channel without the need to increase cannula flow. Low recirculation ratios resulted in increased efficiency, while high recirculation ratios caused slightly reduced CO2removal rates. Acidification of the CO2depleted blood in the recirculation channel caused an increase in CO2removal rate.ConclusionsWe demonstrate a simple and cost effective, yet powerful, “in-vitro” ECCO2R model that can be used as an alternative to animal experiments for many research scenarios. Moreover, in our approach parameters such as hemoglobin level can be modified more easily than in animal models.
Prevalence, antimicrobial susceptibility pattern, and associated factors of Salmonella and Shigella among food handlers in Adigrat University student’s cafeteria, northern Ethiopia, 2018
Authors: Legese Haftom , Kahsay Tsega , Gebrewahd Aderajew , Berhe Brhane , Fseha Berhane , Tadesse Senait , Gebremariam Guesh , Negash Hadush , Mardu Fitsum , Tesfay Kebede , Adhanom Gebre 
Published: September 2020

AbstractBackgroundFood handlers play a significant role in the transmission of foodborne infections.SalmonellaandShigellaare the most common foodborne pathogens and their infections are a major public health problem globally. Thus, this study aimed to determine the prevalence, antimicrobial susceptibility patterns, and associated factors ofSalmonellaandShigellacolonization among food handlers.MethodologyA cross-sectional study was conducted from March to August 2018 at Adigrat University student cafeteria, Northern Ethiopia. Data on socio-demographic and associated factors were collected using a structured questionnaire. Fresh stool samples were collected from 301 food handlers and transported to Adigrat University Microbiology Laboratory. Bacterial isolation and antimicrobial susceptibility test were performed using standard bacteriological methods. Data analysis was performed using SPSS version 22 andP < 0.05 where a corresponding 95% confidence interval was considered statistically significant.ResultsA total of 301 food handlers were included in this study. The majority of study participants were females 265 (88.0%). About 22 (7.3%) and 11 (3.7%) of food handlers were found to be positive forSalmonellaandShigellarespectively. Hand washing after using a bathroom with water only, no hand washing after using the bathroom, no hand washing after touching dirty materials, no hand washing before food handling, and untrimmed fingernails were significant associated factors identified. None of theSalmonellaandShigellaisolates were sensitive to ampicillin, yet low resistance against chloramphenicol, ceftriaxone, and ciprofloxacin was found.ConclusionThe present study revealed that the prevalence ofSalmonellaandShigellaamong food handlers was 22 (7.3%) and 11 (3.7%) respectively. Such colonized food handlers can contaminate food, and drinks and could serve as a source of infection to consumers. This indicates that there is a need for strengthened infection control measures to preventSalmonellaandShigellatransmission in the students’ cafeteria.
Expression of ACE2 and a viral virulence-regulating factor CCN family member 1 in human iPSC-derived neural cells: implications for COVID-19-related CNS disorders
Authors: Kase Yoshitaka , Okano Hideyuki 
Published: September 2020

AbstractIt has been reported that coronavirus disease 2019 (COVID-19) causes not only pneumonia but also systemic inflammations including central nervous system (CNS) disorders. However, little is known about the mechanism that triggers the COVID-19-associated CNS disorders, due to the lack of appropriate experimental systems. Our present study showed that angiotensin-converting enzyme-2 (ACE2), a cellular receptor for SARS-CoV-2, is expressed in human induced pluripotent stem cell (iPSC)-derived neural stem/progenitor cells (hiPSC-NS/PCs) and young neurons. Furthermore, together with database analysis, we found that a viral virulent factor CCN family member 1 (CCN1), which is known to be induced by SARS-CoV-2 infection, is expressed in these cells at basal levels. Considering the role of CCN1 which is known to be involved in viral toxicity and inflammation, hiPSC-NS/PCs could provide an excellent model for COVID-19-associated CNS disorders from the aspect of SARS-CoV-2 infection-ACE2-CCN1 axis. In addition, we identified compounds that reduce CCN1 expression. Collectively, our study using hiPSC-NS/PCs may aid in the development of a therapeutic target for COVID-19-related CNS disorders.
Gender-specific temporal trends in overweight prevalence among Chinese adults: a hierarchical age-period-cohort analysis from 2008 to 2015
Authors: Yang Yinmei , Kelifa Mohammedhamid Osman , Yu Bin , Herbert Carly , Wang Yongbo , Jiang Junfeng 
Published: September 2020

Abstract
Background
As a key health risk, the prevalence of overweight has been strikingly increasing worldwide. This study aimed to disentangle the net age, period, and cohort effects on overweight among Chinese adults by gender.

Methods
Data came from the Chinese General Social Survey from 2008 to 2015, which was a repeated cross-sectional survey (n = 55,726, aged 18 and older). χ2 or t tests were used to estimate the gender disparities in overweight and socioeconomic status (SES). A series of hierarchical age-period-cohort cross-classified random-effects models were performed using SAS version 9.4 to estimate the overall and gender-specific temporal trends of overweight, as well as the association between SES and overweight. Further, a series of line charts were used to present the age and cohort variations in overweight.

Results
After controlling for covariates, significant age and cohort effects were observed among adults in China (b = 0.0205, p < 0.001; b = 0.0122, p < 0.05; respectively). Specifically, inverted U-shaped age effects were identified for both genders, with a high probability of overweight occurring in middle age (b = –0.0012, p < 0.001). Overweight was more prevalent among men than women before 60 years old, and this trend reversed thereafter (b = –0.0253, p < 0.001). Moreover, men born during the war (before 1950) and reform cohorts (after the 1975s) demonstrated a substantial decline in overweight, while men born in 1950–1975 showed an increasing trend in overweight prevalence (b = 0.0378, p < 0.05). However, the cohort effect on women was not statistically significant. Additionally, a higher SES was related to an elevated probability of overweight.

Conclusion
Gender-specific age and cohort effects on the prevalence of overweight were observed among Chinese adults. Both China and other developing countries need to pay attention to the coming obesity challenge and related health inequality. Full life-cycle overweight prevention interventions should focus on middle-aged adults, men born in the war and reform eras, and adults with a higher SES.
Suitable methods for isolation, culture, storage and identification of wheat blast fungus Magnaporthe oryzae Triticum pathotype
Authors: Gupta Dipali Rani , Surovy Musrat Zahan , Mahmud Nur Uddin , Chakraborty Moutoshi , Paul Sanjoy Kumar , Hossain Md. Shaid , Bhattacharjee Pallab , Mehebub Md. Shabab , Rani Kanistha , Yeasmin Rumana , Rahman Mahfuzur , Islam Md Tofazzal 
Published: September 2020

AbstractWheat blast disease caused by a South American lineage of Magnaporthe oryzae Triticum (MoT) pathotype has emerged as a serious threat to wheat production in Bangladesh since its first emergence in 2016. Efficient and suitable methods for isolation, storage, inoculum production and molecular characterization of the pathogen can help in achieving the target of sustainable management of the disease in a relatively short period of time. In this study, we aimed to develop suitable methods for isolation, storage and morphological characterization and molecular identification of MoT isolates collected from the blast-infected wheat fields in Bangladesh. This process included modification of existing protocols that were available for a related fungal pathogen M. oryzae or de novo method development and validation. We developed suitable methods for isolation of MoT from field-infected plant samples using modified monoconidial isolation technique and produced abundant conidia from a single mycelial plate for in vivo pathogenicity assay in a reproducible manner. Cultural and morphological characterization of the isolates revealed that all Bangladeshi MoT isolates are of a single clonal lineage with similar cultural and morphological characters. Molecular detection of isolates with M. oryzae-specific primers Pot1 and Pot2 and MoT-specific primers MoT3F and MoT3R produced bands with the expected size from all wheat-infecting isolates. We also successfully established a PCR-based detection system based on a commercially available detection kit for field-infected leaf and seed samples by detecting Pot2- and MoT3-specific bands. Additionally, the simple method we developed in our study for producing abundant conidia in a very short period of time will be very helpful in studying biology of the wheat blast fungus. This method was also proven to be more user-friendly and cost-effective than previously available methods. Successful characterization of MoT isolates at morphological and molecular levels coupled with detection of the pathogen in infected field and seed lots should be useful for efficient surveillance and management of the fearsome wheat blast disease.
Phase I Study of Cabozantinib and Nivolumab Alone or With Ipilimumab for Advanced or Metastatic Urothelial Carcinoma and Other Genitourinary Tumors
Authors: Apolo Andrea B. , Nadal Rosa , Girardi Daniel M. , Niglio Scot A. , Ley Lisa , Cordes Lisa M. , Steinberg Seth M. , Sierra Ortiz Olena , Cadena Jacqueline , Diaz Carlos , Mallek Marissa , Davarpanah Nicole N. , Costello Rene , Trepel Jane B. , Lee Min-Jung , Merino Maria J. , Bagheri Mohammad Hadi , Monk Paul , Figg William D. , Gulley James L. , Agarwal Piyush K. , Valera Vladimir , Chalfin Heather J. , Jones Jennifer , Streicher Howard , Wright John J. , Ning Yangmin M. , Parnes Howard L. , Dahut William L. , Bottaro Donald P. , Lara Primo N. , Saraiya Biren , Pal Sumanta K. , Stein Mark N. , Mortazavi Amir 
Published: September 2020

PURPOSE We assessed the safety and efficacy of cabozantinib and nivolumab (CaboNivo) and CaboNivo plus ipilimumab (CaboNivoIpi) in patients with metastatic urothelial carcinoma (mUC) and other genitourinary (GU) malignances. PATIENTS AND METHODS Patients received escalating doses of CaboNivo or CaboNivoIpi. The primary objective was to establish a recommended phase II dose (RP2D). Secondary objectives included objective response rate (ORR), progression-free survival (PFS), duration of response (DoR), and overall survival (OS). RESULTS Fifty-four patients were enrolled at eight dose levels with a median follow-up time of 44.6 months; data cutoff was January 20, 2020. Grade 3 or 4 treatment-related adverse events (AEs) occurred in 75% and 87% of patients treated with CaboNivo and CaboNivoIpi, respectively, and included fatigue (17% and 10%, respectively), diarrhea (4% and 7%, respectively), and hypertension (21% and 10%, respectively); grade 3 or 4 immune-related AEs included hepatitis (0% and 13%, respectively) and colitis (0% and 7%, respectively). The RP2D was cabozantinib 40 mg/d plus nivolumab 3 mg/kg for CaboNivo and cabozantinib 40 mg/d, nivolumab 3 mg/kg, and ipilimumab 1 mg/kg for CaboNivoIpi. ORR was 30.6% (95% CI, 20.0% to 47.5%) for all patients and 38.5% (95% CI, 13.9% to 68.4%) for patients with mUC. Median DoR was 21.0 months (95% CI, 5.4 to 24.1 months) for all patients and not reached for patients with mUC. Median PFS was 5.1 months (95% CI, 3.5 to 6.9 months) for all patients and 12.8 months (95% CI, 1.8 to 24.1 months) for patients with mUC. Median OS was 12.6 months (95% CI, 6.9 to 18.8 months) for all patients and 25.4 months (95% CI, 5.7 to 41.6 months) for patients with mUC. CONCLUSION CaboNivo and CaboNivoIpi demonstrated manageable toxicities with durable responses and encouraging survival in patients with mUC and other GU tumors. Multiple phase II and III trials are ongoing for these combinations.
p63-related signaling at a glance
Authors: Fisher Matthew L. , Balinth Seamus , Mills Alea A. 
Published: September 2020

ABSTRACT
p63 (also known as TP63) is a transcription factor of the p53 family, along with p73. Multiple isoforms of p63 have been discovered and these have diverse functions encompassing a wide array of cell biology. p63 isoforms are implicated in lineage specification, proliferative potential, differentiation, cell death and survival, DNA damage response and metabolism. Furthermore, p63 is linked to human disease states including cancer. p63 is critical to many aspects of cell signaling, and in this Cell science at a glance article and the accompanying poster, we focus on the signaling cascades regulating TAp63 and ΔNp63 isoforms and those that are regulated by TAp63 and ΔNp63, as well the role of p63 in disease.
Cell-to-cell and genome-to-genome variability of adenovirus transcription tuned by the cell cycle
Authors: Suomalainen Maarit , Prasad Vibhu , Kannan Abhilash , Greber Urs F. 
Published: September 2020

ABSTRACT
In clonal cultures, not all cells are equally susceptible to virus infection, and the mechanisms underlying this are poorly understood. Here, we developed image-based single-cell measurements to scrutinize the heterogeneity of adenovirus (AdV) infection. AdV delivers, transcribes and replicates a linear double-stranded DNA genome in the nucleus. We measured the abundance of viral transcripts using single-molecule RNA fluorescence in situ hybridization (FISH) and the incoming 5-ethynyl-2′-deoxycytidine (EdC)-tagged viral genomes using a copper(I)-catalyzed azide–alkyne cycloaddition (click) reaction. Surprisingly, expression of the immediate early gene E1A only moderately correlated with the number of viral genomes in the cell nucleus. Intranuclear genome-to-genome heterogeneity was found at the level of viral transcription and, in accordance, individual genomes exhibited heterogeneous replication activity. By analyzing the cell cycle state, we found that G1 cells exhibited the highest E1A gene expression and displayed increased correlation between E1A gene expression and viral genome copy numbers. The combined image-based single-molecule procedures described here are ideally suited to explore the cell-to-cell variability in viral gene expression in a range of different settings, including the innate immune response.
This article has an associated First Person interview with the first author of the paper.
Postnatal development of diving physiology: implications of anthropogenic disturbance for immature marine mammals
Authors: Noren Shawn R. 
Published: September 2020

ABSTRACTMarine mammals endure extended breath-holds while performing active behaviors, which has fascinated scientists for over a century. It is now known that these animals have large onboard oxygen stores and utilize oxygen-conserving mechanisms to prolong aerobically supported dives to great depths, while typically avoiding (or tolerating) hypoxia, hypercarbia, acidosis and decompression sickness (DCS). Over the last few decades, research has revealed that diving physiology is underdeveloped at birth. Here, I review the postnatal development of the body's oxygen stores, cardiorespiratory system and other attributes of diving physiology for pinnipeds and cetaceans to assess how physiological immaturity makes young marine mammals vulnerable to disturbance. Generally, the duration required for body oxygen stores to mature varies across species in accordance with the maternal dependency period, which can be over 2 years long in some species. However, some Arctic and deep-diving species achieve mature oxygen stores comparatively early in life (prior to weaning). Accelerated development in these species supports survival during prolonged hypoxic periods when calves accompany their mothers under sea ice and to the bathypelagic zone, respectively. Studies on oxygen utilization patterns and heart rates while diving are limited, but the data indicate that immature marine mammals have a limited capacity to regulate heart rate (and hence oxygen utilization) during breath-hold. Underdeveloped diving physiology, in combination with small body size, limits diving and swimming performance. This makes immature marine mammals particularly vulnerable to mortality during periods of food limitation, habitat alterations associated with global climate change, fishery interactions and other anthropogenic disturbances, such as exposure to sonar.
Dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) for pretreatment prediction of neoadjuvant chemotherapy response in locally advanced hypopharyngeal cancer
Authors: Guo Wei , Zhang Ya , Luo Dehong , Yuan Huishu 
Published: September 2020

Objective: The aim of this study was to predict response to neoadjuvant chemotherapy (NAC) in patients with locally advanced hypopharyngeal cancer by dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI). Methods: A retrospective study enrolled 46 diagnosed locally advanced hypopharyngeal cancer. DCE-MRI were performed prior to and after two cycles of NAC. The volume transfer constant (Ktrans), extracellular extravascular volume fraction (Ve), and plasma volume fraction (Kep) were computed from primary tumors. DCE-MRI parameters were used to measure tumor response according to the Response Evaluation Criteria in Solid Tumors criteria (RECIST). Results： After 2 NAC cycles, 30 out of 46 patients were categorized into the responder group, whereas the other 16 were categorized into non-responder group. Compared with the pretreatment value, the post-treatment Ktrans and Kep was significantly lower (P < 0.05), but no significant change in Ve (P > 0.05). Compared with non-responders, a notably higher pretreatment Ktrans, Kep, lower post-treatment Ktrans, higher ΔKtrans and ΔKep were observed in responders (all P < 0.05). While the pretreatment Ve, post-treatment Ve, and ΔVe did not differ significantly (P>0.05) between the two groups. The receiver operating characteristic curve analysis revealed that pretreatment Ktrans of 0.202/min is the most optimal cut-off in predicting response to chemotherapy, resulting in an AUC of 0.837 and corresponding sensitivity and specificity of 76.7%, and 81.1%, respectively. Conclusion: DCE-MRI especially pretreatment Ktrans can potentially predict the treatment response to neoadjuvant chemotherapy for hypopharyngeal cancer. Advances in knowledge: Few studies of DCE-MRI on hypopharyngeal cancer treated with chemoradiation reported. The results demonstrate that DCE-MRI especially pretreatment Ktrans may be more potential value in predicting the treatment response to neoadjuvant chemotherapy for hypopharyngeal cancer.