bluuebunny/crossref_metadata_2025_split huggingface dataset:
Anti-obesity Effect of Gold Nanoparticles fromDendropanax morbifera Léveilleby Suppression of Triglyceride Synthesis and Downregulation of PPARγ and CEBPα Signaling Pathways in 3T3-L1 Mature Adipocytes and HepG2 Cells
Authors: Yi Myoung Hi , Simu Shakina Yesmin , Ahn Sungeun , Aceituno Verónica Castro , Wang Chao , Mathiyalagan Ramya , Hurh Joon , Batjikh Indra , Ali Hashmoonah , Kim Yeon-Ju , Kim Sunoh , Yang Deok-Chun 
Published: January 2020

Background:Biosynthesis of gold nanoparticles from medicinal plants has become an interesting strategy in biomedical research due to its exclusive properties including less toxic cellular level through its ecofriendly biological function.Objective:To examine the anti-lipid accumulation effect of spherical gold nanoparticles (size 10-20 nm) synthesized from Dendropanax morbifera Léveille (D-AuNPs) in both 3T3-L1 and HepG2 cells.Method:3T3-L1 preadipocytes and HepG2 hepatocytes were stimulated with cocktail media to generate obese and fatty liver disease models. Cell cytotoxicity and cell proliferation assays were performed in adipocytes at different stages of growth. An anti-lipid accumulation assay was performed in 3T3-L1 obese and HepG2 fatty liver models using different doses of D-AuNPs. Expression of adipogenic genes of PPARγ, CEBPα, Jak2, STAT3, and ap2 and hepatogenic genes PPARα, FAS, and ACC was measured by real-time PCR. In addition, protein expression of PPARγ and CEBPα was evaluated by immunoblotting assay.Result:We found that D-AuNPs (size 10–20 nm) at concentrations up to 100 µg/ml were nontoxic to 3T3-L1 and HepG2 at post-confluent and mature stages. In addition, pretreatment of D-AuNPs at post-confluent stage reduced triglyceride content. In addition, the adipogenesis process was negatively controlled by D-AuNPs, with downregulated PPARγ, CEBPα, Jak2, STAT3, and ap2 expression in 3T3-L1 cells and FAS and ACC levels in HepG2 cells.Conclusion:These data indicated that D-AuNPs exert antiadipogenic properties. We hypothesize that Dendropanax contains a large amount of phenolic compound that coats the surface of gold nanoparticles and has the ability to reduce the excess amount of lipid in both cell lines.
Comparison of the Demographics, Clinical Presentations, Seasonality, and Outcome between Adult Patients with Influenza A and B in Two Seasons (2014-2015 and 2015-2016) with Emphasis on H1N1 Infection Outcome
Authors: Khalil Abdalla , Elhadry Ahmed , Talo Mohamed , Elwakeel Mohamed , Ahmed Tamer , Alshehail Shams , Atteiya Mohamad , Batal Farouk , Sakha Zainab , Azzo Majduldeen , Nazzal Alaa 
Published: January 2020

Background:
Studies comparing Influenza A and B in our community are extremely limited.

Methods:
Adult patients (n=1708) with ILI who visited the outpatient clinics or emergency department with FIA-positive nasal swab in two seasons were included in the analysis.
Data on demographics, clinical presentation, comorbidities, and prolonged illness (revisit after 48 hours with same presentation and no admission), hospital admissions, death, and LOS were collected.
Data on patients tested for H1N1 (309) by Cepheid Xpert Flu Assay (H1N1 positive, 143 [46%]; H1N1 negative, 166 [54%]) were also collected.

Results:
Prolonged illness was more frequent in patients with influenza B than those with influenza A (15.21% and 10.18%, respectively; P=0.002).
There was no significant difference in frequency of total admissions, medical unit or intensive care unit admissions, LOS, and death between patients with influenza A and B.
Total admissions were more frequent in H1N1-positive patients than H1N1-negative patients (23.1% and 13.3%, respectively; P=0.024).
Prolonged illness was more frequent in H1N1-positive patients (23.1%) than H1N1-negative patients (4.8%) (P < 0.001).
There was no statistically significant difference in admission to the medical unit or ICU, death, and LOS between H1N1-positive and H1N1-negative patients.
The peak months of influenza A were December in the first season and October in the second season. April was the peak month of influenza B in both seasons.
There was no statistically significant difference in the outcome of pregnant patients with influenza (either A or B) compared to non-pregnant women with influenza within the same childbearing age (maternal outcome was not studied).

Conclusion:
Prolonged illness was more frequent in flu B than in flu A patients.
Patients with positive H1N1 had more frequent prolonged illness, and total hospital admissions than those with H1N1-negative patients.
There was a different peak month(s) of patients with flu A compared to flu B.
Green-synthesized Zinc Oxide Nanoparticles from Aqueous Root Extract of Dicoma anomala (Sond.) Mitigates Free Radicals and Diabetes-linked Enzymes
Authors: Balogun F.O. , Ashafa A.O.T. 
Published: January 2020

Background:
The emergence of eco-friendly methods for the synthesis of metallic
nanostructures has continued to receive wider acceptance.

Objective:
The study investigated the effect of biologically-synthesized ZnO nanoparticles on free
radicals and carbohydrate-hydrolyzing enzymes.

Methods:
The characterized nanoparticles, DaZnONPs (Dicoma anomala zinc oxide nanoparticles)
were obtained using ultraviolet-visible spectroscopy, transmission electron microscopy, Fourier-
transform infrared spectroscopy (FTIR), energy dispersive spectroscopy, and X-ray diffraction
technique (XRD). The activity of the synthesized nanostructures against 1,1-diphenyl-2-picrylhydrazyl
(DPPH), 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), metal chelating,
alpha-amylase and alpha-glucosidase was determined using standard methods.

Results:
DaZnONPs were observed to be stable, mostly cubical in shape and within the nanometre
size range. Optimum absorption of DaZnONPs was observed at 386 nm. The FTIR analysis indicated
the presence of functional groups arising from alkaloids, flavonoids, tannins, and saponins
(detected in earlier reports) and indicate potential nucleation and stability of the ZnONPs. XRD
result depicted similar patterns of DaZnONPs and standard ZnO spectra, revealing a hexagonal
and crystalline nature of the particles in nanometre range as shown by the obtained peaks.
DaZnONPs inhibited DPPH (0.54 μgmL-1) and alpha-amylase (104.34 μgmL-1) better than quercetin
(349.98 μgmL-1) and acarbose (594. 54 μgmL-1). Meanwhile, the metal chelating effect of
DaZnONPs (30.41 μgmL-1) was observed to be insignificantly (p>0.05) at par with quercetin
(27.81 μgmL-1). The kinetics of alpha-amylase and alpha-glucosidase enzymes by DaZnOnPs was
observed to be non-competitive inhibitions.

Conclusion:
DaZnONPs (as against the bulk extract) could be explored as possible antioxidative
and antihyperglycaemic agents mitigating the adverse effects of free radicals and hyperglycaemia.
Solving the Job Shop Scheduling Problem with an Enhanced Artificial Bee Colony Algorithm through Local Search Heuristic
Authors: Asadzadeh Leila 
Published: January 2020

Aims:
In this paper, we propose an enhanced artificial bee colony (ABC) algorithm for
the job shop scheduling problem.

Background:
Meta-heuristic approaches are widely applied to solve job shop scheduling problem
and find the near optimal solutions in polynomial time.

Objective:
Improving performance of ABC algorithm to solve job shop scheduling problem.
Methods: Using local search heuristics in ABC algorithm.

Results:
The experimental results showed that the proposed algorithm improves the efficiency.

Conclusion:
Proposed algorithm improves efficiency.
Hackathons as Stepping Stones in Health Care Innovation: Case Study With Systematic Recommendations
Authors: Poncette Akira-Sebastian , Rojas Pablo-David , Hofferbert Joscha , Valera Sosa Alvaro , Balzer Felix , Braune Katarina 
Published: January 2020

Background
Until recently, developing health technologies was time-consuming and expensive, and often involved patients, doctors, and other health care professionals only as passive recipients of the end product. So far, users have been minimally involved in the ideation and creation stages of digital health technologies. In order to best address users’ unmet needs, a transdisciplinary and user-led approach, involving cocreation and direct user feedback, is required. In this context, hackathon events have become increasingly popular in generating enthusiasm for user-centered innovation.

Objective
This case study describes preparatory steps and the performance of a health hackathon directly involving patients and health care professionals at all stages. Feasibility and outcomes were assessed, leading to the development of systematic recommendations for future hackathons as a vehicle for bottom-up innovation in health care.

Methods
A 2-day hackathon was conducted in February 2017 in Berlin, Germany. Data were collected through a field study. Collected field notes were subsequently discussed in 15 informal meetings among the research team. Experiences of conducting two further hackathons in December 2017 and November 2018 were included.

Results
In total, 30 participants took part, with 63% (19/30) of participants between 25 and 34 years of age, 30% (9/30) between 35 and 44 years of age, and 7% (2/30) younger than 25 years of age. A total of 43% (13/30) of the participants were female. The participation rate of medical experts, including patients and health care professionals, was 30% (9/30). Five multidisciplinary teams were formed and each tackled a specific health care problem. All presented projects were apps: a chatbot for skin cancer recognition, an augmented reality exposure-based therapy (eg, for arachnophobia), an app for medical neighborhood connectivity, a doctor appointment platform, and a self-care app for people suffering from depression. Patients and health care professionals initiated all of the projects. Conducting the hackathon resulted in significant growth of the digital health community of Berlin and was followed up by larger hackathons. Systematic recommendations for conducting cost-efficient hackathons (n≤30) were developed, including aspects of community building, stakeholder engagement, mentoring, themes, announcements, follow-up, and timing for each step.

Conclusions
This study shows that hackathons are effective in bringing innovation to health care and are more cost- and time-efficient and potentially more sustainable than traditional medical device and digital product development. Our systematic recommendations can be useful to other individuals and organizations that want to establish user-led innovation in academic hospitals by conducting transdisciplinary hackathons.
Analisis Karakteristik Penderita Restless Legs Syndrome pada Pasien Chronic Kidney Disease di Ruang Hemodialisa
Authors: Rahayu Gusri , Malini Hema , Oktarina Elvi 
Published: January 2020

<p><em>Hemodialysis is one of the management of Chronic Kidney Disease (CKD. Hemodialysis can cause various complications, such is Restless Legs Syndrome (RLS). RLS conditions occur due to increased uremic toxic, causing complaints in the form of pain in the upper or lower extremities, burning sensation, movement that can not be controlled or numbness.The purpose of this study was to determine the characteristics of patients with Restless Legs Syndrome in Chronic Kidney Disease patients in the Hemodialysis Room.The design of this study was descriptive with a purposive sampling. The subjects in this study were 32 respondents. The results showed that the characteristics of RLS sufferers are on average in the late adult age range, most genders are male, most High School educators, the average length of dialysis undergoing above 4 years, many RLS sufferers do not work, most comorbidities are diabetes mellitus and hypertension and the average urea level of respondents 105 gr / dl. RLS condition identification needs to be done so that it can be known early so that the severity of RLS can be corrected immediately</em></p><p><em><br /></em></p><p><em><em>Hemodialisismerupakan salah satu penanganan penyakit ginjal kronik atau Chronic Kidney Disease (CKD. Hemodialisis dapat menimbulkan berbagai komplikasi salah satunya adalah Restless Legs Syndrome (RLS). Kondisi RLS terjadi diakibatkan uremic toxic yang meningkat sehingga menimbulkan keluhan berupa rasa nyeri pada ekstremitas atas maupun bawah, rasa terbakar, terjadi pergerakan yang tidak dapat dikontrol maupun rasa kebas. Tujuan penelitian ini adalah untuk mengetahui karakteristik penderita Restless Legs Syndrome pada pasien Chronic Kidney Disease di Ruang Hemodialisa. Desain penelitian ini adalah deskriptif dengan teknik pengambilan sampel yaitu purposive sampling.Subjek dalam penelitian ini berjumlah 32 responden. Hasil penelitian</em> m<em>enunjukkan bahwa karakteristik penderita RLS rata-rata berada dalam rentang usia dewasa akhir, jenis kelamin terbanyak yaitu laki-laki, pendidilkan terbanyak Sekolah Menengah Atas (SMA), rata-rata lama menjalani HD terbanyak diatas 4 tahun, penderita RLS banyak yang tidak bekerja, penyakit penyerta terbanyak yaitu diabetes melitus dan hipertensi dan rata-rata kadar ureum responden 105 gr/dl. Identifikasi kondisi RLS perlu dilakukan sehingga dapat diketahui lebih dini dengan demikian keparahan RLS dapat segera diperbaiki.</em></em></p>
Evaluación <i>in vitro</i> de bacterias marinas para potencial biocontrol en cultivo de moluscos bivalvos
Authors: De la Fuente Mery , Faúndez Víctor 
Published: January 2020

El cultivo de moluscos bivalvos en sistemas controlados requiere un adecuado control de mortalidades en estados larvales debido a infecciones bacterianas. En este trabajo se investigó la actividad de bacterias marinas provenientes de zonas costeras de la provincia de Arauco, Chile para ser usadas como potencial biocontrolador en sistemas artificiales de cultivo. Se realizó el muestreo de diferentes matrices comúnmente asociadas a moluscos bivalvos en su estado natural y se procedió al aislamiento de bacterias marinas. Se evaluó la capacidad de estas bacterias para inhibir 3 bacterias patógenas asociadas normalmente al cultivo de moluscos bivalvos, Vibrio anguillarum, Vibrio splendidus y Vibrio parahaemolyticus. Además, se realizó una caracterización fenotípica de las cepas con actividad inhibitoria, considerando sus propiedades de inocuidad y crecimiento, para la utilización práctica de estas cepas en sistemas de cultivo. De las 70 bacterias aisladas, 3 cepas fueron capaces de inhibir al menos un patógeno bacteriano, logrando crecer en agua marina. La caracterización fenotípica de estas 3 cepas permitió catalogarlas como pertenecientes a los géneros Vibrio y Pseudomonas. Una cepa marina fue totalmente inocua según las características evaluadas in vitro. Por tanto, es factible seleccionar bacterias con propiedades inhibitorias y de inocuidad para un potencial biocontrol en cultivos de moluscos bivalvos en la provincia de Arauco.
MODIFIKASI PATI SUKUN (Artocarpus altilis) MENGGUNAKAN ANHIDRIDA ASAM ASETAT DAN APLIKASINYA PADA PEMBUATAN MIE
Authors: Nurhaeni Nurhaeni , Dwiasmukti Pratiwi , Prismawiryanti Prismawiryanti 
Published: January 2020

Penelitian tentang modifikasi pati sukun (Artocarpus altilis) menggunakan anhidrida asam asetat dan aplikasinya pada pembuatan mie telah dilakukan. Penelitian ini bertujuan untuk mengetahui konsentrasi anhidrida asam asetat terbaik dalam menghasilkan kadar asetil dan derajat substitusi tinggi. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) yaitu konsentrasi anhidrida asam asetat terdiri atas 5 taraf (0,05%; 0,10%; 0,15%; 0,20% dan 0,25%) dilakukan sebanyak 2 kali pengulangan untuk masing-masing taraf sehingga didapatkan 10 unit percobaan. Hasil penelitian menunjukan konsentrasi anhidrida asam asetat terbaik diperoleh pada 0,25% dengan kadar asetil 5,75%, dan derajat substitusi 0,23. Hasil uji organoleptik pada parameter rasa dan tekstur, mie pati sukun asetat lebih disukai dibandingkan dengan mie pati alami. Kata Kunci : Sukun, pati sukun asetat, kadar asetil,derajat substitusi.
PEMBUATAN MEMBRAN SELULOSA ASETAT DARI SELULOSA PELEPAH POHON PISANG
Authors: Husni Dina Apriana Putri , Rahim Erwin Abdul , Ruslan Ruslan 
Published: January 2020

Penelitian ini bertujuan untuk mengetahui penambahan rasio polietilen glikol pada selulosa asetat yang menghasilkan karakteristik membran selulosa asetat terbaik. Selulosa diperoleh dari proses ekstraksi pelepah pohon pisang. Selulosa asetat diperoleh dari sintesis selulosa. Pencetakan membran dilakukan dengan melarutkan selulosa asetat ke dalam pelarut aseton 1:45 (b/v) dan ditambahkan pemlastis PEG dengan variasi 0; 2; 4; 6; dan 8 %. Membran diuji ketebalannya menggunakan micrometer sedangkan untuk kuat tarik dan elongasi diuji dengan Universal Testing Machine. Rendemen selulosa yang diperoleh adalah 62,5 %. Rendemen selulosa asetat dari hasil sintesis adalah 50 %. Berat molekul selulosa asetat 24.961,48 g/mol. Karakteristik membran terbaik diperoleh pada penambahan PEG 8% yang memiliki ketebalan 0.0206 mm, kuat tarik sebesar 20,29 MPa, dan elongasi sebesar 3%.Kata Kunci : Selulosa, Selulosa Asetat, Membran, PEG
PENERAPAN METODE EKSTRAKSI PELARUT DALAM PEMISAHAN MINYAK ATSIRI JAHE MERAH (Zingiber officinale Var.Rubrum)
Authors: Rahmadani Nina , Ruslan Ruslan , Satrimafitrah Pasjan 
Published: January 2020

Telah dilakukan penelitian tentang penerapan metode ekstraksi pelarut dalam pemisahan minyak atsiri jahe merah (Zingiber officinale Var.rubrum). Tujuan penelitian ini adalah untuk mengetahui rasio pelarut n-heksan terhadap serbuk jahe merah yang menghasilkan ekstrak minyak atsiri jahe merah dengan rendemen yang tinggi dan komponen senyawa minyak atsiri jahe merah. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL). Perlakuan yang diterapkan adalah pengaruh rasio pelarut n-heksan terhadap serbuk jahe merah yang terdiri atas lima perbandingan rasio yaitu 1:7, 1:8, 1:9, 1:10, dan 1:11 (b/v) dengan dua kali pengulangan. Parameter yang diamati adalah rendemen ekstrak minyak atsiri jahe merah. Hasil penelitian menunjukkan bahwa rendemen ekstrak minyak atsiri jahe merah yang tertinggi tardapat pada rasio 1:11 sebesar 6,695%. Analisis GC-MS minyak atsiri jahe merah menunjukkan bahwa terdapat 6 komponen senyawa yang dihasilkan. Kata Kunci : Minyak Atsiri Jahe, Ekstraksi, Gas Cromatography-Mass Spektrofotometri (GC-MS)
APLIKASI TEKNIK DIFFUSIVE GRADIENT IN THIN FILM PADA PENENTUAN KONSENTRASI LOGAM BERAT MERKURI (Hg)
Authors: Khairuddin Khairuddin , Ruslan Ruslan 
Published: January 2020

Konsentrasi logam Hg yang tinggi di lingkungan akuatik dapat menyebabkan meningkatnya toksisitas dalam ekosistem air. Oleh karena itu, penentuan distribusi konsentrasi logam berat merkuri (Hg) perlu dilakukan. Salah satu metode efektif yang saat ini digunakan dalam penentuan Hg di lingkungan akuatik adalah menggunakan teknik Diffusive Gradient in Thin Films (DGT). Tujuan penelitian ini adalah membuat dan mengaplikasikan teknik Diffusive Gradient in Thin Film pada kajian logam labil merkuri (Hg) dari lingkungan perairan. Teknik ini merupakan metode passive sampling yang dapat mengakumulasi spesi labil merkuri (Hg) yang berada di lingkungan akuatik. Perangkat DGT terdiri dari bahan plastik berbentuk bulat dengan diameter 2,5 cm yang dapat diisi dengan gel lapisan pengkelat logam, gel poliakrilamida sebagai gel lapisan difusi, serta membran filter 0,45 µm. Hasil penelitian menunjukkan peningkatan jumlah massa logam merkuri yang terikat pada gel lapisan pengikat logam Hg pada variasi waktu kontak. Sementara hasil pengujian dengan variasi ketebalan gel lapisan difusi tidak menghasilkan perbedaan yang signifikan terhadap pengukuran massa Hg yang terikat pada binding gel Aplikasi metode DGT pada penetapan konsentrasi logam merkuri dalam perairan Teluk Palu terukur pada kisaran 3,502 - 3,507 µg/L.Kata Kunci: Merkuri, DGT, Chelex-100, Gel Difusi Poliakrilamida.
KADAR LIKOPEN EKSTRAK KASAR BUAH TOMAT DALAM VCO (VIRGIN COCONUT OIL) PADA PERENDAMAN SUHU RUANG
Authors: Indriani Enni , Ruslan Ruslan , Prismawiryanti Prismawiryanti , Satrimafitrah Pasjan 
Published: January 2020

Likopen merupakan salah satu senyawa karotenoid yang dapat berperan sebagai antioksidan yang memiliki sifat larut dalam minyak karena bersifat lipofilik. Penelitian ini memproduksi likopen yang berasal dari ekstrak kasat buah tomat dengan merendamnya dalam VCO. Tujuan penelitian ini untuk mengetahui rasio terbaik dariekstrak kasar buah tomat terhadap VCO dalam menghasilkan likopen, dan juga waktu ekstraksi untuk menghasilkan konsentrasi likopen tertinggi dari ekstraksi atau perendaman ekstrak kasar buah tomat dalam VCO pada suhu ruang. Rasio antara ekstrak kasar buah tomat dan VCO adalah 1:40, 2:40, 3:40, 4:40, 5:40 (b/v) dengan masa ekstraksi mencapai 7 minggu. Hasil penelitian menunjukkan rasio ekstrak likopen kasar buah tomat terbaik pada penelitian ini yaitu 5:40 dan waktu ekstraksi terbaik adalah pada minggu pertama, dengan kadar likopen tertinggi sebesar 0,283 g.Kata kunci: Ekstrak tomat, likopen, VCO, waktu perendaman
KAJIAN EKTRAKSI GELATIN DARI TULANG IKAN KATOMBO (Selar crumenopthalmus)
Authors: Nurhaeni Nurhaeni , Rauf Rian S , Hardi Jaya 
Published: January 2020

Penelitian ini bermaksud untuk mengetahui waktu perendaman dan rasio tulang ikan katombo dengan larutan asam sitrat 9% terhadap rendemen dan mutu gelatin tulang ikan katombo yang dihasilkan. Hasil penelitian menunjukkan waktu terbaik pada perendaman tulang ikan katombo adalah 60 jam dengan rendemen 6,442%, kadar abu 4,50%, kadar air 8,70%, viskositas 2,93 cP dan kekuatan gel 44,40 mm/kg.s. Rasio terbaik antara tulang ikan katombo terhadap larutan asam sitrat 9% adalah 1:4 (b/v) dengan rendemen 6,938%, kadar abu 3,70%, kadar air 8,70%, viskositas 2,93 cP dan kekuatan gel sebesar 44,30 mm/kg.s.Kata kunci : Gelatin, ikan katombo, ekstraksi, viskositas, kekuatan gel
APLIKASI LIKOPEN TERSALUT MALTODEKSTRIN DARI BUAH TOMAT APEL (Lycopersicum pyriforme) DALAM PENGOLAHAN BUBUR JAGUNG MUDA INSTAN
Authors: Anita Anita , Mappiratu Mappiratu , Sumarni Ni Ketut 
Published: January 2020

Telah dilakukan penelitian tentang Aplikasi Likopen Tersalut Maltodekstrin Dari Buah Tomat Apel (Lycopersicum pyriforme) Dalam Pengolahan Bubur Jagung Muda Instan Fungsional. Tujuan penelitian adalah untuk menentukan masa simpan bubur jagung muda instan fungsional yang diberi likopen tersalut maltodekstrin pada berbagai konsentrasi serta untuk mengetahui pengaruh konsentrasi likopen tersalut maltodekstrin terhadap masa simpan bubur instan fungsional. Pencapaian tujuan digunakan likopen tersalut maltodekstrin pada pembuatan bubur jagung instan dengan konsentrasi 250 ppm, 500 ppm, 750 ppm, 100 ppm, dan 1250 ppm. Bubur instan fungsional disimpan pada suhu ruang selama 49 hari dengan selang waktu pengamatan retensi likopen 7 hari. Hasil yang diperoleh menunjukkan masa simpan bubur jagung muda instan fungsional meningkat dengan meningkatnya penggunaan konsentrasi likopen tersalut maltodekstrin. Masa simpan terlama (51,95 hari) ditemukan pada penggunaan likopen tersalut maltodekstrin 1250 ppm. Kata kunci: tomat, likopen, bubur jagung, masa kadaluarsa