bluuebunny/crossref_metadata_2025_split huggingface dataset:
Stilling and Recovery of the Surface Wind Speed Based on Observation, Reanalysis, and Geostrophic Wind Theory over China from 1960 to 2017
Authors: Zhang Zhengtai , Wang Kaicun 
Published: February 2020

AbstractSurface wind speed (SWS) from meteorological observation, global atmospheric reanalysis, and geostrophic wind speed (GWS) calculated from surface pressure were used to study the stilling and recovery of SWS over China from 1960 to 2017. China experienced anemometer changes and automatic observation transitions in approximately 1969 and 2004, resulting in SWS inhomogeneity. Therefore, we divided the entire period into three sections to study the SWS trend, and found a near-zero annual trend in the SWS in China from 1960 to 1969, a significant decrease of −0.24 m s−1 decade−1 from 1970 to 2004, and a weak recovery from 2005 to 2017. By defining the 95th and 5th percentiles of daily mean wind speeds as strong and weak winds, respectively, we found that the SWS decrease was primarily caused by a strong wind decrease of −8% decade−1 from 1960 to 2017, but weak wind showed an insignificant decreasing trend of −2% decade−1. GWS decreased with a significant trend of −3% decade−1 before the 1990s; during the 1990s, GWS increased with a trend of 3% decade−1 whereas SWS continued to decrease with a trend of 10% decade−1. Consistent with SWS, GWS demonstrated a weak increase after the 2000s. After detrending, both SWS and GWS showed synchronous decadal variability, which is related to the intensity of Aleutian low pressure over the North Pacific. However, current reanalyses cannot reproduce the decadal variability and cannot capture the decreasing trend of SWS either.
Age Differences and Similarities in Associated Stressors and Outcomes Among Young, Midlife, and Older Adult Family Caregivers
Authors: Koumoutzis Athena , Cichy Kelly E. , Dellmann-Jenkins Mary , Blankemeyer Maureen 
Published: February 2020

Background and Objectives Few studies have simultaneously compared caregivers in all stages of the adult life course. This study examined age differences in associations among primary stressors (caregiver burden which includes hours of provided care and number of activities of daily living and instrumental activities of daily living performed), secondary stressors (financial and employment strains), and caregiver outcomes (emotional strain and physical strain). Research Design Using Pearlin’s Stress Process Model (1990) and the Caregiving in the United States 2015 dataset, 1,156 caregivers were identified (including 278 young adults aged 18–39 years, 464 midlife adults aged 40–59 years, and 414 older adults aged 60–80 years). Results Post hoc analyses revealed that compared to older adults, young adults reported less caregiver burden, less physical strain, and greater financial strain. Linear regression analyses revealed associations between caregiver burden and financial strain with emotional and physical strain for all respondents. Discussion and Implications Findings emphasize the need for age-specific interventions.
Health technology assessment–based approach to flow cytometric immunophenotyping of acute leukemias: a literature classification
Authors: Gaipa Giuseppe , Erba Eugenio , Danova Marco , Mazzini Giuliano , Venditti Adriano , Buldini Barbara , Specchia Giorgina , Maglia Oscar , Kunkl Annalisa , Ciriello Maria Matilde , Arpinati Mario , Mannelli Francesco , Lanza Francesco , Riccioni Roberta , Pistotti Vanna , Apolone Giovanni ,   on behalf of the Società Italiana di Citometria (GIC)
Published: February 2020

Objective: Acute leukemia (AL) is a broad, heterogeneous group of malignant diseases. The diagnostic workup of AL is based on several clinical and laboratory findings, including flow cytometric immunophenotyping. However, the role of this assay in the diagnosis of AL has not been systematically investigated. The aim of this study was to determine the accuracy and utility of flow cytometric immunophenotyping in the identification, characterization, and staging of AL. Methods: We performed a systematic selection and classification of the literature since 1980, focused on flow cytometric immunophenotyping of AL. We applied a 6-variables model to cover both the technical capabilities and the clinical value of flow cytometric immunophenotyping in the diagnosis of AL. Results: Using 3 key words (acute leukemia, immunophenotyping, flow cytometry), we screened the literature from January 1985 to April 2015 in PubMed and Embase databases and found 1010 articles. A total of 363 were selected and submitted to the expert panel, which selected a final data set of 248 articles to be analyzed. Of these, 160 were focused on clinical and biological issues, 55 were technical articles, and 31 were reviews. These 248 articles were then analyzed according to the 6-variables model and definitively classified. Conclusions: We assessed the literature on flow cytometric immunophenotyping of AL over 3 decades as the first step toward an evidence-based analysis of the impact of this technology on the clinical management of patients with AL.
Participatory Water Resource Management in the Bhutan Himalayan Foothill Environment of Baksa District, Assam
Authors: Saha Sourav , Deka Nityananda , Bhagabati Abani Kumar 
Published: February 2020

Participatory water resource management assumes great significance in the changing context of fresh water availability and use. The forces of market economy, in most cases, have transformed the rural social scenario in such a way that community participation in resource management has become almost a far cry. But the Himalayan foothill area in Assam sets a good example of management and sharing of water resources by the communities living in the area in eco-friendly and socially just ways. This study is an attempt to analyse the traditional management practices called dong-bandh system evolved by the local people to harvest and share the stream water for agricultural and domestic purposes. Based on data collected from secondary sources and generated through primary survey, the study focuses on the organizational set-up of the community participation, water distribution policies, conflict resolution and perception of the people on dong–bandh governance. The challenges faced by this traditional system during the recent period have also been discussed.
From Streets and Squares to Radical Political Emancipation? Resistance Lessons from Athens during the Crisis
Authors: Hadjimichalis Costis 
Published: February 2020

The current crisis with imposed austerity measures hit Greece in 2009. People in large cities such as Athens were the first victims. Resistance took various forms including mobilizations and fights in the streets and squares of Athens. Although to some extent spontaneous in the beginning, these mobilizations were not without political preparation (at least for some participants) and this partially explains both their intensity and stability and the violent police reaction. Resistance and anti-austerity mobilizations were outcomes of non-politicized people coming together with various more organized political forces, such as unions, non-unionized temporary workers, anti-racist and anti-global movements, members of the European Social Forum-Greek section, small leftist and anarchist groups, plus larger political parties of the left such as SYRIZA, which succeeded in forming a wider radical alliance. In this alliance the role of radical social movements, including urban movements, has been decisive. During the past 10 to 15 years dozens of urban grass-roots movements emerged in the Athens metropolitan region. Partly as a response to projects related to the 2004 Olympic Games, and partly in response to chronic socio-spatial inequalities and injustices in various neighborhoods, these movements were radical in nature, multi-class in social base and quite militant in terms of tactics. And since 2009, the crisis acted as catalyst, and radical social movements made the crucial link between sectoral/local struggles and those arising from wider socio-spatial contradictions and injustices aiming at political change. The paper critically evaluates these events, putting them in theoretical and comparative perspective, trying to understand the limits and the lessons-so-far from such an experience and asks whether they point to a wider radical political emancipation.
Improvements in patients’ quality of life following treatment with intravesical hyaluronic acid (Cystistat 40 mg) for bladder pain syndrome and recurrent UTIs
Authors: Brophy Thomas D , Fowler Sue , Clarke Louise , Thompson Andy 
Published: February 2020

Introduction: Intravesical hyaluronic acid (Cystistat) is indicated for a variety of chronic cystitis conditions including bladder pain syndrome, recurrent bacterial urinary tract infections and radiation or chemical cystitis. Previously published studies have predominantly assessed the outcome in terms of bladder symptoms (frequency/urgency/nocturia/pain) or in the case of recurrent bacterial urinary tract infection, by microbiological response. The aim of this study was to assess improvement in patients’ quality of life following treatment with intravesical Cystistat. Materials and methods: Patients’ referred for treatment with intravesical Cystistat for either bladder pain syndrome or recurrent bacterial urinary tract infection completed the King’s health questionnaire. Patients were treated with a 6-week course of Cystistat by one of two specialist urology nurses. If treatment was effective further instillations were given every 2–4 weeks for up to 6 months. King’s health questionnaire scores were repeated after 6 weeks and 6 months and were analysed. Results: Twenty patients (18 women, 2 men) were included: 8 bladder pain syndrome, 12 recurrent bacterial urinary tract infections. There were four treatment failures within 6 weeks, of whom three were being treated for recurrent bacterial urinary tract infections. Of the 16 patients who continued with treatment beyond 6 weeks, 12 have 6-month scores available. The average initial King’s health questionnaire score was 500.8 (534.1 for the recurrent bacterial urinary tract infection group, 450.8 for the bladder pain syndrome group). After six treatments average King’s health questionnaire scores improved to 426.3 (457.7 for recurrent bacterial urinary tract infections, 372.4 for bladder pain syndrome). After 6 months, average scores significantly improved to 278 overall (303.2 ( P<0.05) for the recurrent bacterial urinary tract infections group, 252.8 ( P>0.05) for the bladder pain syndrome group). Conclusion: Intravesical Cystistat should be considered in all patients with bladder pain syndrome and recurrent bacterial urinary tract infections. We have shown a significant, clinically important, improvement in patients’ quality of life in refractory bladder pain syndrome and recurrent bacterial urinary tract infections. In future Cystistat could be considered as an alternative to long-term low-dose antibiotic prophylaxis. Level of evidence: 4
SLC20A1 at the Interface between Macrophages and Red Blood Cells
Authors: Quattrone Federica , Gawish Riem , Martins Rui , Gorki Anna-Dorothea , Watzenboeck Martin , Hladik Anastasiya , Lakovits Karin , Rebsamen Manuele , Girardi Enrico , Superti-Furga Giulio , Knapp Sylvia 
Published: February 2020

Red blood cells (RBCs) comprise 84% of our body's cells and have the essential function of transporting oxygen from the lungs to all organs. The daily production rate of RBCs is enormous and the pathways mediating this process are quite complex. One important aspect hereby is the synthesis of hemoglobin and heme, the iron-containing prosthetic group that enables hemoglobin to bind oxygen.RBCs differentiate within the erythropoietic niche, which consists of erythroid precursors and specialized macrophages. These so-called nursing macrophages provide nutrients and iron, a fundamental component of heme.
The precise mechanisms of heme transport within a cell and the potential of heme transfer between cells is not completely understood. To get insights into heme transport we took advantage of the cytotoxic capacity of heme and performed a CRISPR-Cas9 loss of function screen by focusing on SLC transporters. We identified SLC20A1 as an essential protein mediating heme toxicity and verified that intracellular heme levels as well as heme-induced downstream gene inductions were reduced in the absence of Slc20a1. Based on these evidences we hypothesized that SLC20A1 might be involved in the trafficking of heme, possibly important during erythropoiesis, since mice lacking Slc20a1 exhibit embryonic lethality due to liver apoptosis and anemia (Beck L. et al., PLoS One 2013).
To test the biological role of Slc20a1 in adult mice, we conditionally deleted Slc20a1 using tamoxifen in ERT2-Cre Slc20a1fl animals and observed the spontaneous development of severe anemia and splenomegaly within 2 weeks after tamoxifen administration. We further discovered that the anemia-related expansion of red pulp macrophages (RPM) consisted predominantly of non-recombined wild type cells, whereas successfully recombined (Slc20a1 deficient) cells seemed stuck in the monocytic stage. These data suggest that Slc20a1 might be involved in the differentiation and maturation of monocytes to RPMs. When we performed a specific Slc20a1 deletion in nursing macrophages (CD169 Cre), mice neither showed signs of anemia, nor experienced impaired recovery upon anemia induction following phlebotomy or induction of hemolysis. However, deleting Sc20a1 specifically in the erythroid compartment using Slc20a1fl mice crossed to erythropoietin receptor-cre (EpoR-Cre) animals, resulted in embryonic lethality. Mice died around E12.5 due to severe anemia. Analysis of E11.5 animals disclosed erythroid precursors to be arrested in the pro-erythroblast stage.These data suggest that SLC20A1 is a protein involved in heme-mediated toxicity and possibly in the trafficking of heme with strong impact on fetal and adult erythropoiesis.

Disclosures
No relevant conflicts of interest to declare.
Efficacy of Mesenchymal Stem Cells in Bronchiolitis Obliterans Syndrome after Allogeneic HSCT: A Multicenter Prospective Cohort Study
Authors: Chen Shan , Zhao Ke , Lin Ren , Wang Shunqing , Fan Zhiping , Huang Fen , Chen Xiaoyong , Nie Danian , Du Xin , Guo Ziwen , Lin Dongjun , Xuan Li , Xu Na , Sun Jing , Xiang Andy Peng , Liu Qifa 
Published: February 2020

Background:
Bronchiolitis obliterans syndrome (BOS) after allogeneic hematopoietic stem cell transplantation (allo-HSCT) is a devastating complication with limited therapeutic options. We aimed to assess the efficacy and safety of mesenchymal stem cells (MSCs) in BOS after allo-HSCT.
Methods:
Eighty-one allo-HSCT recipients with BOS diagnosed within 6 months were enrolled in this multicenter prospective cohort study. The choice of prednisone and azithromycin combined with or without MSCs was based on patient preferences (MSC n=49, non-MSC n=32). The primary endpoint was response rate at 3 months, defined as the proportion of patients achieving FEV1 improvement or steroid sparing. This trial was registered at ClinicalTrials.gov, NCT02543073.
Results:
Response rate was 35/49 patients (71%, 95% confidence intervals [CI] 59 to 84%) (FEV1 improvement n=10, steroid sparing n=25) and 14/32 (44%, 95% CI 27 to 61%) (FEV1 improvement n=3, steroid sparing n=11) in the MSC and non-MSC group, respectively (p=0.013). The adjusted odds ratio of response in the MSC group compared with the non-MSC group was 3.32 (95% CI 1.21-9.11; p=0.02). The addition of MSCs was associated with a better difference for change in FEV1 rate of decline, compared to non-MSC group (53 ml/months, 2 to 103; p=0.040). The 3-year overall survival post-diagnosis was 70.6% (95% CI 55.9 to 85.3%) and 58.2% (95% CI 36.1 to 78.5%) in the MSC and non-MSC group, respectively (p=0.21). Clinical improvement was accompanied by a significant increase of interleukin (IL)-10-producing CD5+ B cells. The incidences of infection and leukemia relapse were no significant difference between two groups. Multiple infusions of MSCs were well-tolerated with no serious adverse events.
Interpretation:
MSCs might be an effective and safe therapy for BOS patients after allo-HSCT. Our study strengthens evidence for clinical practice of MSC therapy in BOS. These data also offer insight into potential biological mechanisms of MSC treatment and support further investigation in larger randomized controlled trials.

Disclosures
No relevant conflicts of interest to declare.
Effect of Dasatinib Vs Imatinib in the Treatment of Pediatric Philadelphia Chromosome-Positive Acute Lymphoblastic Leukemia: A Randomized, Open-Label, Multicenter Study of the Chinese Children's Cancer Group
Authors: Shen Shuhong , Chen Xiaojuan , Cai Jiaoyang , Jie Yu , Gao Ju , HU Shaoyan , Zhai Xiaowen , Liang Changda , Ju Xiuli , Jiang Hua , Jin Runming , Wu Xuedong , Wang Ningling , Tian Xin , Pan Kaili , Jiang Hui , SUN Lirong , Fang Yongjun , Li Chi Kong , Hu Qun , Yang Minghua , Zhu Yiping , Li Chunfu , Yang Jun J. , Zhang Hui , Tang Jing-Yan , Zhu Xiaofan , Pui Ching-Hon 
Published: February 2020

OBJECTIVE To determine whether dasatinib given at 80 mg/m2 is more effective than imatinib at 300 mg/m2 to improve event-free survival of children with Philadelphia chromosome-positive ALL, in the context of intensive chemotherapy without prophylactic cranial irradiation.
DESIGN, SETTING, AND PARTICIPANTS This open-label phase III randomized study was conducted at 20 hospitals in China. Enrollment began in January 2015 and randomization was stopped in October 2018 when the early stopping criterion of the trial was met. Patients aged between 0 and 18 years were recruited. Of the 225 patients with the diagnosis, 35 declined and 1 died before treatment.
INTERVENTIONS Patients were randomized to receive daily dasatinib (n=92) or imatinib (n=97) continuously for the entire duration of ALL therapy from the time of diagnosis made during remission induction to the end of continuation therapy.
MAIN OUTCOMES AND MEASURES The primary outcome was event-free survival, analyzed by intent-to-treat. The secondary outcomes were relapse, toxic death, and overall survival.
RESULTS With a median follow-up of 26.1 months (IQR 16.3-34.1), the 4-year event-free survival rate was 71.0% (95% CI 56.2-89.6) in the dasatinib group and 48.9% (95% CI 32.0-74.5, p=0.005) in the imatinib group (hazard ratio 2.36, 95% CI 1.27-4.40, p=0.007). The 4-year cumulative risk of any relapse was 19.8% (95% CI 4.2-35.4) in the dasatinib group and 34.4% (95% CI 15.6-53.2) in the imatinib group (p=0.01), while the 4-year cumulative risk of an isolated central-nervous-system relapse was 2.7% (95% CI 0.0-8.1) in the dasatinib group and 8.4% (95% CI -1.2-15.6) in the imatinib group (p=0.06). There were no significant differences in the frequency of severe toxicities between the two treatment groups.
CONCLUSION AND RELEVANCE Intensive chemotherapy including dasatinib at 80 mg/m2 per day yielded superior results in the treatment of Philadelphia chromosome-positive ALL compared to imatinib at 300 mg/m2 per day and provided excellent control of central-nervous-system leukemia without the use of prophylactic cranial irradiation.

Disclosures
No relevant conflicts of interest to declare.
Stat3-/- Alloreactive Donor T Cells Reduce FAO but Increase Leakage of ROS in Response to PD-1 Signaling Triggered By Host-Tissue PD-L1, Leading to Prevention of Gvhd While Preserving GVL Effect
Authors: Wang Xiaoqi , Yang Shijie , Wang Yating , Wu Xiwei , Salas Martha , Song Qingxiao , Qin Hanjun , Nasri Ubaydah , Riggs Arthur D. , Martin Paul J , Zhang Xi , Zeng Defu 
Published: February 2020

The GVHD pathogenicity of alloreactive T cells is associated with enhanced glycolysis or enhanced fatty acid oxidation (FAO). PD-1 signaling in T cells inhibits glycolysis but augments FAO. Host tissue PD-L1 interaction with PD-1 on activated alloreactive T cells is not able to effectively prevent acute GVHD mediated by donor CD4+ and CD8+ T cells together, although it can reduce the severity of the disease. Using a murine HCT model of C57BL/6 donor to BALB/c recipient, we show that although WT T cells (CD4+ and CD8+ T cells together) induced lethal GVHD at 1-5 x106/mouse, the same dose of Stat3-/- donor T cells induced little signs of acute or chronic GVHD because of induction of T cell anergy, exhaustion and apoptosis, but with retention of a strong GVL effect. This induction of tolerance depends on donor T cell PD-1 interaction with host-tissue PD-L1, because Stat3-/- T cells induce severe lethal GVHD in the PD-L1-/- recipients and in WT recipients treated with blocking anti-PD-1 mAb. The tolerance of Stat3-/- donor T cells induced by PD-1 signaling is associated with lower FAO and mitochondria membrane potential (MMP) but higher ROS leakage, with little difference in glycolysis, as compared to non-tolerant WT T cells. In the absence of PD-1 signaling, Stat3-/- T cells manifest markedly enhanced glycolysis and loss of tolerance. These results indicate that activated donor T cell PD-1 interaction with host-tissue PD-L1 reduces glycolysis and reduces their GVHD capacity. In addition, absence of mitochondrial pStat3 in Stat3-/- donor T cells leads to reduced FAO and MMP but increased ROS leakage, resulting in effective anergy, exhaustion, and apoptosis of tissue infiltrating donor T cells and prevention of GVHD. At the same time, the reduced expression of PD-L1 in recipient lymphoid tissues allows donor T cell expansion and strong GVL activity. Our studies have revealed novel roles of mitochondria pStat3 in regulating PD-1 signaling, T cell metabolism and tolerance. (Grant support: R01AI066008 to Zeng) .

Disclosures
No relevant conflicts of interest to declare.