bluuebunny/crossref_metadata_2025_split huggingface dataset:
Non-cuttable material created through local resonance and strain rate effects
Authors: Szyniszewski Stefan , Vogel Rene , Bittner Florian , Jakubczyk Ewa , Anderson Miranda , Pelacci Manuel , Chinedu Ajoku , Endres Hans-Josef , Hipke Thomas 
Published: July 2020

AbstractWe have created a new architected material, which is both highly deformable and ultra‐resistant to dynamic point loads. The bio-inspired metallic cellular structure (with an internal grid of large ceramic segments) is non-cuttable by an angle grinder and a power drill, and it has only 15% steel density. Our architecture derives its extreme hardness from the local resonance between the embedded ceramics in a flexible cellular matrix and the attacking tool, which produces high-frequency vibrations at the interface. The incomplete consolidation of the ceramic grains during the manufacturing also promoted fragmentation of the ceramic spheres into micron-size particulate matter, which provided an abrasive interface with increasing resistance at higher loading rates. The contrast between the ceramic segments and cellular material was also effective against a waterjet cutter because the convex geometry of the ceramic spheres widened the waterjet and reduced its velocity by two orders of magnitude. Shifting the design paradigm from static resistance to dynamic interactions between the material phases and the applied load could inspire novel, metamorphic materials with pre-programmed mechanisms across different length scales.
Long-term effects of medical management on growth and weight in individuals with urea cycle disorders
Authors: Posset Roland , Garbade Sven F. , Gleich Florian , Gropman Andrea L. , de Lonlay Pascale , Hoffmann Georg F. , Garcia-Cazorla Angeles , Nagamani Sandesh C. S. , Baumgartner Matthias R. , Schulze Andreas , Dobbelaere Dries , Yudkoff Marc , Kölker Stefan , Zielonka Matthias ,   Urea Cycle Disorders Consortium (UCDC), Ah Mew Nicholas , Berry Susan A. , McCandless Shawn E. , Coughlin Curtis , Enns Gregory , Gallagher Renata C. , Burrage Lindsay C. , Seminara Jennifer , Harding Cary O. , Burgard Peter , Le Mons Cynthia , Merritt J. Lawrence , Stricker Tamar , Bedoyan Jirair K. , Berry Gerard T. , Diaz George A. , Wong Derek , Tuchman Mendel , Waisbren Susan , Weisfeld-Adams James D. ,   European registry and network for Intoxication type Metabolic Diseases (E-IMD), Burlina Alberto B. , Leão Teles Elisa , Pedrón-Giner Consuelo , Lund Allan M. , Dionisi-Vici Carlo , Williams Monique , Mütze Ulrike , Karall Daniela , Blasco-Alonso Javier , Couce Maria L. , Sykut-Cegielska Jolanta , Augoustides-Savvopoulou Persephone , Ruiz Gomez Angeles , Barić Ivo , Schiff Manuel , Chien Yin-Hsiu , Lindner Martin , Chabrol Brigitte , Skouma Anastasia , Zeman Jiri , Sokal Etienne , Santer René , Eyskens Francois , Freisinger Peter , Peña-Quintana Luis , Roland Dominique , Cortès-Saladelafont Elisenda , Djordjevic Maja 
Published: July 2020

AbstractLow protein diet and sodium or glycerol phenylbutyrate, two pillars of recommended long-term therapy of individuals with urea cycle disorders (UCDs), involve the risk of iatrogenic growth failure. Limited evidence-based studies hamper our knowledge on the long-term effects of the proposed medical management in individuals with UCDs. We studied the impact of medical management on growth and weight development in 307 individuals longitudinally followed by the Urea Cycle Disorders Consortium (UCDC) and the European registry and network for Intoxication type Metabolic Diseases (E-IMD). Intrauterine growth of all investigated UCDs and postnatal linear growth of asymptomatic individuals remained unaffected. Symptomatic individuals were at risk of progressive growth retardation independent from the underlying disease and the degree of natural protein restriction. Growth impairment was determined by disease severity and associated with reduced or borderline plasma branched-chain amino acid (BCAA) concentrations. Liver transplantation appeared to have a beneficial effect on growth. Weight development remained unaffected both in asymptomatic and symptomatic individuals. Progressive growth impairment depends on disease severity and plasma BCAA concentrations, but cannot be predicted by the amount of natural protein intake alone. Future clinical trials are necessary to evaluate whether supplementation with BCAAs might improve growth in UCDs.
Persistence of coastal upwelling after a plunge in upwelling-favourable wind
Authors: Jung Jihun , Cho Yang-Ki 
Published: July 2020

AbstractUnprecedented coastal upwelling off the southern coast of the Korean Peninsula was reported during the summer of 2013. The upwelling continued for more than a month after a plunge in upwelling-favourable winds and had serious impacts on fisheries. This is a rare phenomenon, as most coastal upwelling events relax a few days after the wind weakens. In this study, observational data and numerical modelling results were analysed to investigate the cause of the upwelling and the reason behind it being sustained for such an extended period. Coastal upwelling was induced by an upwelling-favourable wind in July, resulting in the dynamic uplift of deep, cold water. The dynamic uplift decreased the steric sea level in the coastal region. The sea level difference between the coastal and offshore regions produced an intensified cross-shore pressure gradient that enhanced the surface geostrophic current along the coast. The strong surface current maintained the dynamic uplift due to geostrophic equilibrium. This positive feedback between the dynamic uplift and geostrophic adjustment sustained the coastal upwelling for a month following a plunge in the upwelling-favourable wind.
High expression of TCN1 is a negative prognostic biomarker and can predict neoadjuvant chemosensitivity of colon cancer
Authors: Liu Guang-jie , Wang Yao-jie , Yue Meng , Zhao Lian-mei , Guo Yong-Dong , Liu Yue-ping , Yang Hui-chai , Liu Fang , Zhang Xue , Zhi Liang-hui , Zhao Jing , Sun Yan-Hua , Wang Gui-ying 
Published: July 2020

AbstractTranscobalamin (TCN1) is a vitamin B12 (cobalamin)-binding protein that regulates cobalamin homeostasis. Recent studies and bioinformatic analyses have found that TCN1 is highly expressed in cancer tissues and is associated with tumour aggressiveness and poor prognosis. The present study aimed to detect TCN1 as a novel biomarker for prognosis and chemosensitivity of colon cancer. Next-generation sequencing showed that TCN1 was one of several upregulated mRNAs in colon cancer, which was verified by further bioinformatics analyses. Western blotting (n = 9) and quantitative real time polymerase chain reaction (qRT-PCR, n = 30) revealed that TCN1 was highly expressed in colon cancer tissues at both the protein and mRNA level. A total of 194 cases of colon cancer were examined by immunohistochemistry and revealed that TCN1 expression level was related to advanced stages (P < 0.005). Kaplan–Meier analysis verified that patients with lower TCN1 expression usually had longer overall survival (P = 0.008). In addition, TCN1 was highly expressed in pulmonary metastatic tumour tissues (n = 37, P = 0.025) and exhibited higher levels in right-sided colon cancer than in left-sided colon cancer (P = 0.029). TCN1 expression in specimens that had received neoadjuvant chemotherapy decreased compared with that in colonoscopy biopsy tissues (n = 42, P = 0.009). Further bioinformatics analyses verified that apoptosis pathways might have a role in high TCN1 expression. All the studies revealed that TCN1 expression in colon cancer was significantly associated with malignant biological behaviour. Therefore, TCN1 could be used as a novel biomarker for colon cancer aggressiveness and prognosis and might also be a potential biomarker for predicting neoadjuvant chemosensitivity.
Neuronal activity remodels the F-actin based submembrane lattice in dendrites but not axons of hippocampal neurons
Authors: Lavoie-Cardinal Flavie , Bilodeau Anthony , Lemieux Mado , Gardner Marc-André , Wiesner Theresa , Laramée Gabrielle , Gagné Christian , De Koninck Paul 
Published: July 2020

AbstractThe nanoscale organization of the F-actin cytoskeleton in neurons comprises membrane-associated periodical rings, bundles, and longitudinal fibers. The F-actin rings have been observed predominantly in axons but only sporadically in dendrites, where fluorescence nanoscopy reveals various patterns of F-actin arranged in mixed patches. These complex dendritic F-actin patterns pose a challenge for investigating quantitatively their regulatory mechanisms. We developed here a weakly supervised deep learning segmentation approach of fluorescence nanoscopy images of F-actin in cultured hippocampal neurons. This approach enabled the quantitative assessment of F-actin remodeling, revealing the disappearance of the rings during neuronal activity in dendrites, but not in axons. The dendritic F-actin cytoskeleton of activated neurons remodeled into longitudinal fibers. We show that this activity-dependent remodeling involves $$\text {Ca}^{2+}$$Ca2+ and NMDA receptor-dependent mechanisms. This highly dynamic restructuring of dendritic F-actin based submembrane lattice into longitudinal fibers may serve to support activity-dependent membrane remodeling, protein trafficking and neuronal plasticity.
Microdomains form on the luminal face of neuronal extracellular vesicle membranes
Authors: Matthies Doreen , Lee Nathanael Y. J. , Gatera Ian , Pasolli H. Amalia , Zhao Xiaowei , Liu Hui , Walpita Deepika , Liu Zhe , Yu Zhiheng , Ioannou Maria S. 
Published: July 2020

AbstractExtracellular vesicles (EVs) are important mediators of cell-to-cell communication and have been implicated in several pathologies including those of the central nervous system. They are released by all cell types, including neurons, and are highly heterogenous in size and composition. Yet much remains unknown regarding the biophysical characteristics of different EVs. Here, using cryo-electron microscopy (cryoEM), we analyzed the size distribution and morphology of EVs released from primary cortical neurons. We discovered massive macromolecular clusters on the luminal face of EV membranes. These clusters are predominantly found on medium-sized vesicles, suggesting that they may be specific to microvesicles as opposed to exosomes. We propose that these clusters serve as microdomains for EV signaling and play an important role in EV physiology.
Injection of seminal fluid into the hemocoel of honey bee queens (Apis mellifera) can stimulate post-mating changes
Authors: Jasper W. Cameron , Brutscher Laura M. , Grozinger Christina M. , Niño Elina L. 
Published: July 2020

AbstractHoney bee queens undergo dramatic behavioral (e.g., reduced sexual receptivity), physiological (e.g., ovary activation, ovulation, and modulation of pheromone production) and transcriptional changes after they complete mating. To elucidate how queen post-mating changes are influenced by seminal fluid, the non-spermatozoa-containing component of semen, we injected queens with semen or seminal fluid alone. We assessed queen sexual receptivity (as measured by likelihood to take mating flights), ovary activation, worker retinue response (which is influenced by queen pheromone production), and transcriptional changes in queen abdominal fat body and brain tissues. Injection with either seminal fluid or semen resulted in decreased sexual receptivity, increased attractiveness of queens to workers, and altered expression of several genes that are also regulated by natural mating in queens. The post-mating and transcriptional changes of queens receiving seminal fluid were not significantly different from queens injected with semen, suggesting that components in seminal fluid, such as seminal fluid proteins, are largely responsible for stimulating post-mating changes in queens.
Sequence analysis of nonulosonic acid biosynthetic gene clusters in Vibrionaceae and Moritella viscosa
Authors: Halsør Marie-Josée Haglund , Altermark Bjørn , Ræder Inger Lin Uttakleiv 
Published: July 2020

AbstractNonulosonic acid (NulO) biosynthesis in bacteria is directed by nab gene clusters that can lead to neuraminic, legionaminic or pseudaminic acids. Analysis of the gene content from a set mainly composed of Aliivibrio salmonicida and Moritella viscosa strains reveals the existence of several unique nab clusters, for which the NulO products were predicted.
This prediction method can be used to guide tandem mass spectrometry studies in order to verify the products of previously undescribed nab clusters and identify new members of the NulOs family.
Recurrent probabilistic neural network-based short-term prediction for acute hypotension and ventricular fibrillation
Authors: Tsuji Toshio , Nobukawa Tomonori , Mito Akihisa , Hirano Harutoyo , Soh Zu , Inokuchi Ryota , Fujita Etsunori , Ogura Yumi , Kaneko Shigehiko , Nakamura Ryuji , Saeki Noboru , Kawamoto Masashi , Yoshizumi Masao 
Published: July 2020

AbstractIn this paper, we propose a novel method for predicting acute clinical deterioration triggered by hypotension, ventricular fibrillation, and an undiagnosed multiple disease condition using biological signals, such as heart rate, RR interval, and blood pressure. Efforts trying to predict such acute clinical deterioration events have received much attention from researchers lately, but most of them are targeted to a single symptom. The distinctive feature of the proposed method is that the occurrence of the event is manifested as a probability by applying a recurrent probabilistic neural network, which is embedded with a hidden Markov model and a Gaussian mixture model. Additionally, its machine learning scheme allows it to learn from the sample data and apply it to a wide range of symptoms. The performance of the proposed method was tested using a dataset provided by Physionet and the University of Tokyo Hospital. The results show that the proposed method has a prediction accuracy of 92.5% for patients with acute hypotension and can predict the occurrence of ventricular fibrillation 5 min before it occurs with an accuracy of 82.5%. In addition, a multiple disease condition can be predicted 7 min before they occur, with an accuracy of over 90%.
Everyday bodily movement is associated with creativity independently from active positive affect: a Bayesian mediation analysis approach
Authors: Rominger Christian , Fink Andreas , Weber Bernhard , Papousek Ilona , Schwerdtfeger Andreas R. 
Published: July 2020

AbstractPrevious (predominantly) laboratory studies reported positive relations of physical activity (or everyday bodily movement) with executive functioning, some even showed effects on creative thinking. Furthermore, positive-activated affect was found to be positively associated with everyday bodily movements and creativity. The mechanisms, however, underlying these relationships are poorly understood. The aim of this study was twofold: Firstly, we investigated whether everyday bodily movement was associated with creative performance. Secondly, we examined if positive-activated affect may mediate the association between bodily movement and creative performance. In a sample of 79 participants everyday bodily movement was recorded during five consecutive days using accelerometers. Creativity in the figural and verbal domain was assessed with performance tests, along with self-reported positive-activated affect as a trait. Findings revealed that creativity, positive-activated affect, and everyday bodily movement were associated with each other. However, positive-activated affect did not mediate the association between everyday bodily movement and creative performance. The pattern of findings argues for shared variance between bodily movement and creativity (fluency and originality) that is largely independent from variations in positive-activated affect.
Salt templated and graphene nanoplatelets draped copper (GNP-draped-Cu) composites for dramatic improvements in pool boiling heat transfer
Authors: Rishi Aniket M. , Kandlikar Satish G. , Gupta Anju 
Published: July 2020

AbstractWe demonstrate a novel technique to achieve highly surface active, functional, and tunable hierarchical porous coated surfaces with high wickability using a combination of ball milling, salt-templating, and sintering techniques. Specifically, using ball-milling to obtain graphene nanoplatelets (GNP) draped copper particles followed by salt templated sintering to induce the strength and cohesiveness to the particles. The salt-templating method was specifically used to promote porosity on the coatings. A systematic study was conducted by varying size of the copper particles, ratio of GNP to copper particles, and process parameters to generate a variety of microporous coatings possessing interconnected pores and tunnels that were observed using electron microscopy. Pool boiling tests exhibited a very high critical heat flux of 289 W/cm2 at a wall superheat of just 2.2 °C for the salt templated 3 wt% GNP draped 20 µm diameter copper particles with exceedingly high wicking rates compared to non-salt-templated sintered coatings. The dramatic improvement in the pool boiling performance occurring at a very low surface temperature due to tunable surface properties is highly desirable in heat transfer and many other engineering applications.
FKS1 mutation associated with decreased echinocandin susceptibility of Aspergillus fumigatus following anidulafungin exposure
Authors: e Silva Ana Pinto , Miranda Isabel Marcos , Branco Joana , Oliveira Patricia , Faria-Ramos Isabel , Silva Raquel M. , Rodrigues Acácio Gonçalves , Costa-de-Oliveira Sofia 
Published: July 2020

AbstractInvasive aspergillosis (IA) is a potentially lethal infection that affects mostly immunocompromised patients caused by Aspergillus fumigatus. Echinocandins are a second-line therapy against IA, used as a salvage therapy as well as for empirical or prophylactic therapy. Although they cause lysis of growing hyphal tips, they are considered fungistatic against molds. In vivo echinocandins resistance is uncommon; however, its wide clinical use could shortly lead to the emergence of A. fumigatus resistance. The aims of the present work was to assess the development of reduced echinocandins susceptibility phenotype by a A. fumigatus strain and to unveil the molecular mechanism underlying such phenotype. We induced in vitro cross-resistance to echinocandins following exposure of A. fumigatus to anidulafungin. Stability of the resistant phenotype was confirmed after removal of anidulafungin pressure. The FKS1 gene was partially sequenced and a E671Q mutation was found. A computational approach suggests that it can play an important role in echinocandin resistance. Given the emerging importance of this mechanism for clinical resistance in pathogenic fungi, it would be prudent to be alert to the potential evolution of this resistant mechanism in Aspergillus spp infecting patients under echinocandins therapeutics.
Role of internal tide mixing in keeping the deep Andaman Sea warmer than the Bay of Bengal
Authors: Jithin A. K. , Francis P. A. 
Published: July 2020

AbstractVertical profiles of temperature obtained from various hydrographic datasets show that deep waters (below 1,200 m) in the Andaman Sea are warmer (about 2 °C) than that of the Bay of Bengal. As a result, the biochemical properties in the deep waters also exhibit significant differences between these two basins. Higher temperature in the deep waters of Andaman Sea compared to the BoB had been widely attributed to the enclosed nature of the Andaman Sea. In this study, we show that strong tidal energy dissipation in the Andaman Sea also plays an important role in maintaining the higher temperatures in the deep waters. Dissipation rates inferred from the hydrographic data and internal tide energy budget suggests that the rate of vertical mixing in the Andaman Sea is about two-orders of magnitude larger than that in the Bay of Bengal. This elevated internal tide induced vertical mixing results in the efficient transfer of heat into the deeper layers, which keeps the deep Andaman Sea warm. Numerical experiments conducted using a high-resolution setup of Regional Ocean Modelling System (ROMS) further confirm the effect of tidal mixing in the Andaman Sea.
The complete mitochondrial genome of Indian gaur, Bos gaurus and its phylogenetic implications
Authors: Kamalakkannan Ranganathan , Bhavana Karippadakam , Prabhu Vandana R. , Sureshgopi Dhandapani , Singha Hijam Surachandra , Nagarajan Muniyandi 
Published: July 2020

AbstractThe gaur is the largest extant cattle species and distributed across South and Southeast Asia. Around 85% of its current global population resides in India, however there has been a gradual decrease in the gaur population over the last two decades due to various anthropogenic activities. Mitochondrial genome is considered as an important tool for species identification and monitoring the populations of conservation concern and therefore it becomes an obligation to sequence the mitochondrial genome of Indian gaur. We report here for the first time 16,345 bp mitochondrial genome of four Indian gaur sequenced using two different approaches. Mitochondrial genome consisted of 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes, and a control region. Among the 37 genes, 28 were positioned on the H-strand and 9 were positioned on the L-strand. The overall base composition appeared to be 33.5% A, 27.2% T, 25.9% C and 13.4% G, which yielded a higher AT content. The phylogenetic analysis using complete mitochondrial genome sequences unambiguously suggested that gaur is the maternal ancestor of domestic mithun. Moreover, it also clearly distinguished the three sub species ofB. gaurusi.e.B. gaurus gaurus, B. gaurus readeiandB. gaurus hubbacki. Among the three sub species,B. gaurus gauruswas genetically closer toB. gaurus readeias compared toB. gaurus hubbacki. The findings of our study provide an insight into the genetic structure and evolutionary history of Indian gaur.
Simple liquid chromatography-electrospray ionization ion trap mass spectrometry method for the quantification of galacto-oxylipin arabidopsides in plant samples
Authors: Genva Manon , Andersson Mats X. , Fauconnier Marie-Laure 
Published: July 2020

AbstractA simple and sensitive method to quantify five different arabidopsides by HPLC—ion trap mass spectrometry in complex plant samples was developed and validated. Arabidopsides are oxidized galactolipids first described in Arabidopsis thaliana but also produced by other plant species under stress conditions. External calibration was performed using arabidopsides purified from freeze-thawed Arabidopsis leaves. Lipids were extracted and pre-purified on an SPE silica column before HPLC–MS analysis. Arabidopsides were separated on a C18 column using a gradient of mQ water and acetonitrile:mQ water (85:15) supplemented with formic acid (0.2%) and ammonium formate (12 mM). The method was validated according to European commission decision 2002/657/CE. LOD, LOQ, linearity, intra-day and inter-day precision and accuracy, selectivity, matrix effects and recoveries were determined for the five metabolites. The established method is highly selective in a complex plant matrix. LOD and LOQ were, respectively, in the range 0.098–0.78 and 0.64–1.56 µM, allowing the arabidopside quantification from 25.6–62.4 nmol/g fresh weight. Calibration curve correlation coefficients were higher than 0.997. Matrix effects ranged from -2.09% to 6.10% and recoveries between 70.7% and 109%. The method was successfully applied to complex plant matrixes: Arabidopsis thaliana and Nasturtium officinale.