bluuebunny/crossref_metadata_2025_split huggingface dataset:
circ-ANKS1B facilitates osteosarcoma cell proliferation and invasiveness via upregulating Ki-67 expression by sponging miR-149-5p
Authors: Yang Hao , Liu Yujie , Li Chao , Liu Yilin , Zhao Liang , Wang Yuqiang , Zhang Yang , Zhang Min , Wang Limin 
Published: February 2020

Abstract
Background: Mounting evidence has shown that Circular RNAs (circRNAs) are associated with initiation and progression of human cancers. However, the expression and function of circRNAs in the development of osteosarcoma (OS) remain unclear.Methods: In this study, the expression profiles of circRNA circ-ANKS1B in OS were identified through qRT-PCR and in situ hybridization (ISH). The relationships between expression of circ-ANKS1B and clinicopathological features of OS patients was analyzed. Cell proliferation potential, migration and invasion ability of OS cells were evaluated through CCK8, colony formation, transwell and wound healing assays in vitro. Xenograft nude mouse experiment was performed to investigate tumor formation ability in vivo. The downstream regulated microRNA of circ-ANKS1B was proved via qRT-PCR and dual-luciferase reporter.Results: We found expression of circ-ANKS1B was markedly overexpressed in OS cell lines and tumor tissues, and high expression of circ-ANKS1B was correlated with advanced TNM stage and poor prognosis of OS patients. The results of functional experiments showed that depletion of circ-ANKS1B could inhibit proliferation and invasion ability of OS cells in vitro, and tumor formation ability in vivo. Further mechanistic studies revealed that circ-ANKS1B could sponge endogenous miR-149-5p and partially reversed the suppressive effect of miR-149-5p in OS cells. Furthermore, we demonstrated that circ-ANKS1B regulated Ki-67 expression by sponging miR-149-5p. Conclusions: In summary, our data showed that circ-ANKS1B accelerated cell growth and invasion in OS by sponging miR-149-5p and regulating Ki-67.
Double diapering facilitates hip maturation in newborns: a prospective randomised control study
Authors: Lee Wei C. , Kao Hsuan K. , Chen Mei C. , Yang Wen E , Chu Shih M. , Chang Chia H. 
Published: February 2020

Abstract
Background Maintaining the hips in flexion and abduction posture is a common recommendation to facilitate hip maturation in infants. Double diapering helps to maintain a baby’s hips in flexion and abduction posture, but the efficacy of double diapers in hip development has never been verified. We investigated whether double diapering results in greater improvement of the alpha angle in the first months of life. Methods This prospective randomised control study enrolled newborns with Graf type IIa hips. Babies were assigned to the double-diaper or single-diaper group. Parents were instructed on proper hip positioning, except for diapering. Change in the alpha angle from newborn to 1 month after birth, rate of improvement to bilateral Graf type I hips in 1 month, and number of ultrasound examinations and orthopaedic clinic visits in the first year were compared between the two groups. For babies with bilateral IIa hips, we used the averaged data from both hips, and for babies with unilateral IIa hips, we used the data from the IIa hips. Results Seventy newborns with 102 type IIa hips were included from January to December 2017. They were allocated to the double-diaper group (N=33) and single-diaper group (N=37). With a comparable sex ratio, gestational age, and newborn alpha angle, the double-diaper group had a greater increase of alpha angles in 1 month than the single-diaper group (+7.9° vs. +5.2°, t-test, p=0.011). Twenty-eight babies in the double-diaper group (84.8%) and 20 babies in the single-diaper group (54.1%) improved to having bilateral Graf type I hips (chi-square test, p=0.006). Subsequent clinical visits and hip ultrasounds before 1 year were significantly reduced in the double-diaper group. Conclusions Our results support double diapering for enhancing hip maturation in babies with Graf type IIa hips. The amount of improvement may not indicate double diapering as a treatment for hip dysplasia, but its effects in immature hips are noteworthy in reducing further clinical and ultrasound follow-ups.
Adipogenic differentiation of human adipose-derived mesenchymal stem cells regulated by microRNA-26b-5p/TCF-4
Authors: Luo Yadong , Ji Huan , Cao Yan , Ding Xu , Li Meng , Song Haiyang , Li Sheng , Wang Chenxing , Wu Heming , Meng Jian , Du Hongming 
Published: February 2020

Abstract
Background: Our study was designed to investigate the role of miR-26b-5p on TCF-4, affecting the adipogenic differentiation of human adipose-derived mesenchymal stem cells (hADMSCs). METHODS: The adipogenic differentiation of hADMSCs was induced by adipogenic medium for 6 days (d). Bioinformatic and dual-luciferase analyses were used to confirm the relationship between TCF-4 and miR-26b-5p. Immunofluorescence was used to detect the effect of miR-26b-5p on TCF-4 and β-catenin in hADMSCs transfected with miR-26b-5p mimic and inhibitor. Mimic, inhibitor, and small interfering RNA (siRNA) transfected in hADMSCs to against LEF1 and β-catenin. Quantitative real-time PCR and western blotting were used to examine the adipogenic markers and Wnt/β-catenin pathway at the mRNA and protein levels, respectively. Immunofluorescence was performed to locate β-catenin. RESULTS: hADMSCs could differentiate toward adipocytes by the adipogenic medium. The results of bioinformatic and dual-luciferase analyses show that TCF-4 is a potential target of miR-26b-5p. The immunofluorescence intensity of TCF4 and β-catenin were inhibited by miR-26b-5p in hADMSCs. Overexpression of miR-26b-5p promotes the adipogenic differentiation of hADMSCs. Overexpression of TCF-4 and β-catenin inhibits the adipogenic differentiation of hADMSCs. The adipogenic differentiation of hADMSCs that promoted by knocking down TCF4 could be weakened by low-expression of miR-26b-5p. The stimulative effect of β-catenin low-expression in adipogenic differentiation was inhibited by miR-26b-5p inhibitor. Conclusions: miR-26b-5p is a negative regulator to inhibit TCF-4 directly, and then inactivated Wnt/β-catenin pathway, which promotes the adipogenic differentiation of hADMSCs in vitro.
Transcriptome analysis of miRNAs expression reveals novel insights into root formation under Root restriction cultivation in grapevine (Vitis vinifera L.)
Authors: Li Hui , Gao Zhen , Zahid Muhammad Salman , Li Dong Mei , Javed Hafiz Umer , Wang Lei , Song Shi ren , Zhao Li Ping , Xu Wen Ping , Zhang Cai Xi , Ma Chao , Wang Shi Ping 
Published: February 2020

Abstract
Background: Root restriction cultivation not only promotes maturation and quality of fruit, but optimizes the architecture of root, especially in enhancing the occurrence of adventitious and lateral roots. While the molecular mechanism of this phenotype is still unknown. Results: In this study, the development of roots was observed at 12 different time points under Root restriction and normal cultivations (control). Root phenotype showed a significantly different feature start from the seventh sampling, mainly in the increasing of adventitious roots numbers, degeneration of root tip and subsequent mass of lateral roots development. The 7th and 12th sampling of two different cultivations, named nR7, nR12, RR7, and RR12, were selected for small RNA sequencing. 214,439,588 raw reads were obtained and 168,741,687 clean reads were remained after the quality control steps, and finally got a total of 153 known miRNAs, and 119 predicted novel miRNAs. The predicted novel miRNAs blasted with the miRVIT and miRBase databases simultaneously. 96 new family members of grapevine miRNA and 23 grapevine-specific novel miRNAs were obtained. Differentially expressed miRNAs (DEMs) analysis showed that 26 and 33 miRNAs were differentially expressed in two different cultivation models (RR7 vs nR7; RR12 vs nR12), while 26 and 32 DEMs were obtained in different root development stages (nR12 vs nR7; RR12 vs RR7). MiRNA expression levels analysis found that conserved miRNA s were in higher expression level than novel miRNAs. The predicted target genes of DEMs were annotated on a variety of biological processes, and 24 participated in root development. An analysis of vvi-miR160 family members revealed that vvi-miR160c was highly expressed in grapevine root, indicating the potential role of miR160c in root development, which in accordance with the previous report in Arabidopsis . Conclusions: Multiple miRNAs were jointly to regulate root development on Root restriction condition, mainly in lateral root development. And the specific expression of vvi-miR160c in the apex may be the main cause of apical degradation. Moreover, there were multiple miRNAs related to biotic or abiotic stresses, which indicated that multiple minor stresses exist in root development after Root restriction cultivation.
Does serum follicle-stimulating hormone during controlled ovarian hyperstimulation predict to ovarian response and reproduction potential in IVF/ICSI clinical practice?
Authors: Ma Yujia , Sun Bo , Hu Linli , Wang Fang , Sun Ying-Pu 
Published: February 2020

Abstract
Background: Although serum basal follicle stimulating hormone (FSH) is widely used to evaluate the ovarian response, the necessity of levels of serum FSH during the controlled ovarian hyperstimulation (COH) is controversy. When the ovarian response to COH is suboptimal due to the insufficient dose of FSH, which is often adjusted in subsequent treatment accordingly, we could detect serum FSH levels and considering that exogenous FSH is inadequate to optimal FSH threshold. We, therefore, aim to evaluate the association between the ovarian response and the difference value of serum FSH concentration in the first five days of ovarian stimulation. Methods: In this retrospective single-center study, patients were enrolled for first IVF/ICSI during the period from August 2015 to December 2017. The COH only included gonadotrophin-releasing hormone agonist (GnRH-a) protocols in which endogenous serum FSH values were suppressed, and stimulated with 150IU fixed-dose recombinant FSH (rFSH) during the first five days. Patients met all inclusion criteria were selected: age ≤ 40 years, body mass index (BMI) ≤ 32 kg/m2, regular menstruation cycle of 21-35 days and non-ovarian factor infertility. Groups were divided by the amount of oocytes collection as follows: (A) poor responders (n=27), (B) normal responders (n=638), (C) hyper responders (n=205). A multivariable logistic regression model was performed to evaluate the relationship between the ovarian response and difference value of serum FSH levels during the first five days of ovarian stimulation.Result(s): The difference value of serum FSH level (ΔFSH) between the sixth day and the first day during ovarian stimulation was measured as the primary outcome. Mean serum ΔFSH levels between groups B and C were 7.45 and 6.87, which had significant differences (p=0.0259). ΔFSH was stratified in quartiles as below: (a) ΔFSH≤5.16, (b) ΔFSH 5.16-7.11, (c) 7.11-9.09, (d) ΔFSH˃9.09. After adjusted by potential confounding factors, there was no relationship exists between ΔFSH levels and ovarian response.Conclusion(s): There is no relevance between the ovarian response and ΔFSH in the 150 IU fixed dose rFSH treatment protocol during COH. Serum FSH might not be used as an effective predictor for ovarian response and reproduction potential in IVF/ICSI clinical practice.
Teaching a DISCHARGE Framework to Hospital Teams to Improve Transitions of Care
Authors: Trivedi Shreya P. , Lipkin Mack , Schwartz Mark D. 
Published: February 2020

Abstract
Background: Residents typically learn about managing transitions of care as part of the informal curriculum in an ad-hoc, reactive manner. Learning may be enhanced by using a framework to proactively practice addressing key domains for a patient soon-to-be-discharged from the hospital. We developed such an evidence-based framework, DISCHARGE , as a cognitive aid. Using this framework, we implemented and evaluated a workshop designed for hospital teams to learn addressing key components of discharging a patient.Methods: All members of 8 Internal Medicine teams across 4 rotations were invited to attend an hour-long workshop ranging from September 2017 - February 2018. Participants completed a retrospective, pre-post survey on their perceived change in discharge-related behaviors. We evaluated the perceived effectiveness of the workshop with a retrospective pre-post questionnaire. We used Wilcoxon signed-rank tests for pairwise comparisons to access perceived changes in discharge behaviors.Results: A total of 90 of 140 team members (64%) attended the workshop and 79 of the 90 (87%) completed the questionnaire. The session was effective in increasing the likelihood of addressing patient-centered behaviors at discharge (mean 1.4 improvement on a 5-point scale, P<0.001, R>0.5). In addition, senior residents and attendings projected they were more likely to discuss the importance of discharge planning with the team early in a rotation. Interns noted they were more comfortable asking the team for help in carrying out a discharge plan (p<0.001, R>0.5).Conclusions: Teaching teams a cognitive aid to practice managing hospital discharges may increase the likelihood of addressing important domains for their patients. Incorporating the team allows for explicit alignment for priorities and communication. Further study is needed to document how such learning is translated into discharge practice.
Statistical methods to support diagnosing rare diseases
Authors: Pilz Guenter F. , Weber Frank , Mueller Werner G. , Schaefer Juergen R. 
Published: February 2020

Abstract
Background Far too often, one meets patients who went for years or even decades from doctor to doctor, without getting a valid diagnosis. This brings pain to millions of patients and their families, not to speak of the enormous costs. Often patients do not know well enough which factors (or combinations thereof) trigger their problems. Results If conventional methods fail, we propose the use of statistics and algebra to give doctors much more precise inputs from patients. We propose statistical regression for independent triggering factors for medical problems, and “balanced incomplete block designs” for non-independent factors. These methods might change a useless statement like “I feel very tired after meals” to a much more valuable “After a meal with many carbohydrates, but few vegetables, a moderate physical activity will usually force me into a wheel-chair”. In order to show that these methods do work, we briefly describe a real case in which these methods helped to solve a 60 year old problem in a patient, and give some more examples where these methods might be very useful. Discussion In this paper, we present a way of getting medical diagnoses when the methods in medicine are insufficient, too time consuming, or very expensive. By asking the patient to conduct tests (often at home) according to a very well-prepared schedule, statistics can use regression analysis to identify the factors (or combinations thereof) which trigger or worsen the patient’s problems. This (very inexpensive) analysis can often give the patient’s doctor(s) a very good and precise input for their diagnosis. Conclusions While regression is used in clinical medicine, it seems to be widely unknown among diagnosing doctors. In finding the reason(s) of rare diseases, doctors face very tough problems. So they deserve to know all tools which could offer some help. This can save the health systems much money, and the patients also a lot of pain.
Availability and Uptake of Community-Based Rehabilitation and Inclusive Development Services in Southwestern Uganda: Experiences of Children with Spina Bifida, Caretakers and Health Professionals
Authors: Ganshanga Ambrose , Asasira Justus 
Published: February 2020

Abstract
It is over a decade since services for children with Spina bifida were established in Uganda which has increased the survival rate after spina bifida repair through follow ups. Such children have significant disability and other complications which damage the central nervous system. The consequences of this are more profound in low-income countries if post-operative and long-term care interventions are not adhered to. This study explored the availability and uptake of community-based rehabilitation and inclusive development services to children with Spina bifida in Southwestern Uganda.
STAT3 signaling pathway could be involved in the progress of cognitive dysfunction caused by ligature-induced periodontitis
Authors: Hu Yi , Zhang Xu , Zhang Jing , Li Huxiao , Xia Xinyi , Qiu Che , Liao Yue , Chen Huiwen , Song Zhongchen , Zhou Wei 
Published: February 2020

Abstract
Background: Increasing evidence suggests a causal link between periodontitis and Alzheimer’s disease (AD). Systemic inflammation initiated by periodontitis may mediate the development of AD. Our study aims to investigate the effect of ligature-induced periodontitis on cognitive function and the role of STAT3 in this process.Materials and Methods: Ligature-induced periodontitis was established, and the rats were treated intraperitoneally with/without the pSTAT3 inhibitor cryptotanshinone (CTS, 5 mg/kg). Alveolar bone resorption and periodontal inflammation were detected by micro-computed tomography (micro-CT) analysis and histopathological evaluation. The expression of cytokines (IL-1β, IL-6, IL-8, IL-21) in the periphery was evaluated by RT-PCR and ELISA. Locomotor activity and cognitive function were evaluated by the open field test and the Morris water maze test, respectively. The activation of microglia and astrocytes was assessed by immunohistochemistry. In the cortex, the cytokines mentioned above were also detected, while the activation of the STAT3 signalling pathway (JAK2, STAT3, and pSTAT3) was assessed by Western blotting. The expression of amyloid precursor protein (APP) and its key secretase enzymes was evaluated by RT-PCR, while the total Aβ and the ratio of Aβ1-40/1-42 were measured via ELISA.Results: In periodontal ligature rats, significant alveolar bone resorption and local inflammatory cell infiltration were present. Apparent increases in inflammatory cytokines (IL-1β, IL-6, IL-8 and IL-21) were detected; this change was relieved by the pSTAT3 inhibitor CTS. Additionally, spatial learning and memory function was impaired, while locomotor activity was not affected. Activated microglia and astrocytes were found in the cortex and hippocampus. In the cortex, these inflammatory cytokines showed similar changes when the STAT3 signalling pathway was activated. The processing of APP by β- and γ-secretases was promoted, and both the total Aβ and the ratio of Aβ1-40/1-42 were upregulated. Changes in the brain were also attenuated by CTS.Conclusions: Ligature-induced periodontitis in rats resulted in systemic inflammation and further impairments in cognitive function, leading to AD-like pathology. The activation of the STAT3 signalling pathway may play an important role in this process by increasing inflammatory load in both the periphery and brain, promoting neuroinflammation and modulating APP processing. This study may provide novel insights into periodontal intervention strategies and potential targets for AD prevention.
Evaluation the influence of core stability exercise on standing stability of hemiplegic cerebral palsy
Authors: Karimi mohammad 
Published: February 2020

Abstract
Background: Cerebral palsy (CP) is one of the most common neurological disorders influences the abilities of the subjects to stand and walk. Various kinds of exercise are recommended to improve the stability of CP subjects during quiet standing. However, there is no evidence regarding the effects of core stability exercise on stability of CP subjects. Therefore, the aim of this research was to determine the efficiency of this exercise on stability of CP subjects.Method: Two group of normal and CP children were recruited in this study (10 subjects). The stability of the subjects was determined by use of a Kistler forceplate. The stability of the subjects was measured by Approximate entropy (ApEn) based on COP sways. The dynamic stability was evaluated by Berge balance scale. Ashworth scale was used to determine the spasticity of lower limb muscles groups. Results: The mean value of ApEn of CP patients in the anteroposterior and mediolateral directions were 0.465±0.11 and 0.426±0.99, respectively compared to 1.02±0.11 and 0.426±0.099 for normal subjects. There was a significant increase in ApEn of CP subjects before and after exercise (p-value<0.05). The mean value of Berge scale was 46.2±5.77 and 51.87±3.9 before and after exercise, respectively.Conclusion: As lower value of ApEn associated with decrease in complexity, higher rigidity and unstability, it can be concluded that CP subjects were unstable than normal subjects. Use of core stability exercise improves complexity of the system and improves the stability of the subjects due to its effect on muscular spasm.
Endoscopic assisted key-hole surgery for subacute subdural hematoma evacuation in emergency situations
Authors: Sun guo-chen , Li Chong , Tang Hao , Yi Ke-fan , Li Fang-ye , Xu Bai-nan , yu xin-guang 
Published: February 2020

Abstract
Background Endoscopic removal of subacute subdural hematoma is seldom performed, but there is no standard technique till date. We investigated whether a simple endoscopic method is effective for the evacuation of subacute subdural hematoma. Methods A total of 51 patients with subacute subdural hematoma requiring surgery were enrolled in this study. Endoscopic hematoma evacuation was performed through a small bone window for 22 patients. Hematoma evacuation by open surgery was performed in 29 patients. Pre- and postoperative Glasgow coma scale (GCS), operation time, displacement of midline, and intraoperative blood loss were recorded and analyzed. Results The median time from incision to suture completion was 40 min (range: 31.25–43.75 min) for endoscopic group, and 70 min (range: 65–80 min) for open surgery group (P<0.01). The average total blood loss was 50(30-50) ml for endoscopic group, and 250(200-300) ml for open surgery group (P<0.01). No patient showed post-operative re-bleeding in both groups. The mean preoperative mid-line displacement for the two groups was 11.51±3.51mm for study group vs 10.47±2.93mm for control group (P>0.05). Mid-line displacement showed significant improvement on the day after surgery to 5.29±1.91mm for study group vs 6.75±1.37mm for control group (P<0.01). At 1-month follow-up, the midline was normal in both groups. Computed tomography revealed almost no residual hematoma, representing an average evacuation rate of 100% in both groups. The mean preoperative GCS score was 13(12.25,15) for study group vs 13(12,14) for control group(P>0.05). GCS score on the day after the operation was improved to 15 for each patient in endoscopic surgery group, while 15(14,15)in the open surgery group (P<0.01). Conclusions Endoscopic subacute subdual hematoma removal through a small bone window achieved a satisfactory hematoma removal with minimally invasive method when compared to open surgery.