bluuebunny/crossref_metadata_2025_split huggingface dataset:
Variation among 532 genomes unveils the origin and evolutionary history of a global insect herbivore
Authors: You Minsheng , Ke Fushi , You Shijun , Wu Zhangyan , Liu Qingfeng , He Weiyi , Baxter Simon W. , Yuchi Zhiguang , Vasseur Liette , Gurr Geoff M. , Ward Christopher M. , Cerda Hugo , Yang Guang , Peng Lu , Jin Yuanchun , Xie Miao , Cai Lijun , Douglas Carl J. , Isman Murray B. , Goettel Mark S. , Song Qisheng , Fan Qinghai , Wang-Pruski Gefu , Lees David C. , Yue Zhen , Bai Jianlin , Liu Tiansheng , Lin Lianyun , Zheng Yunkai , Zeng Zhaohua , Lin Sheng , Wang Yue , Zhao Qian , Xia Xiaofeng , Chen Wenbin , Chen Lilin , Zou Mingmin , Liao Jinying , Gao Qiang , Fang Xiaodong , Yin Ye , Yang Huanming , Wang Jian , Han Liwei , Lin Yingjun , Lu Yanping , Zhuang Mousheng 
Published: May 2020

AbstractThe diamondback moth, Plutella xylostella is a cosmopolitan pest that has evolved resistance to all classes of insecticide, and costs the world economy an estimated US $4-5 billion annually. We analyse patterns of variation among 532 P. xylostella genomes, representing a worldwide sample of 114 populations. We find evidence that suggests South America is the geographical area of origin of this species, challenging earlier hypotheses of an Old-World origin. Our analysis indicates that Plutella xylostella has experienced three major expansions across the world, mainly facilitated by European colonization and global trade. We identify genomic signatures of selection in genes related to metabolic and signaling pathways that could be evidence of environmental adaptation. This evolutionary history of P. xylostella provides insights into transoceanic movements that have enabled it to become a worldwide pest.
Spider venom-derived peptide induces hyperalgesia in Nav1.7 knockout mice by activating Nav1.9 channels
Authors: Zhou Xi , Ma Tingbin , Yang Luyao , Peng Shuijiao , Li Lulu , Wang Zhouquan , Xiao Zhen , Zhang Qingfeng , Wang Li , Huang Yazhou , Chen Minzhi , Liang Songping , Zhang Xianwei , Liu Jing Yu , Liu Zhonghua 
Published: May 2020

AbstractThe sodium channels Nav1.7, Nav1.8 and Nav1.9 are critical for pain perception in peripheral nociceptors. Loss of function of Nav1.7 leads to congenital insensitivity to pain in humans. Here we show that the spider peptide toxin called HpTx1, first identified as an inhibitor of Kv4.2, restores nociception in Nav1.7 knockout (Nav1.7-KO) mice by enhancing the excitability of dorsal root ganglion neurons. HpTx1 inhibits Nav1.7 and activates Nav1.9 but does not affect Nav1.8. This toxin produces pain in wild-type (WT) and Nav1.7-KO mice, and attenuates nociception in Nav1.9-KO mice, but has no effect in Nav1.8-KO mice. These data indicate that HpTx1-induced hypersensitivity is mediated by Nav1.9 activation and offers pharmacological insight into the relationship of the three Nav channels in pain signalling.
Large mass-independent sulphur isotope anomalies link stratospheric volcanism to the Late Ordovician mass extinction
Authors: Hu Dongping , Li Menghan , Zhang Xiaolin , Turchyn Alexandra V. , Gong Yizhe , Shen Yanan 
Published: May 2020

AbstractVolcanic eruptions are thought to be a key driver of rapid climate perturbations over geological time, such as global cooling, global warming, and changes in ocean chemistry. However, identification of stratospheric volcanic eruptions in the geological record and their causal link to the mass extinction events during the past 540 million years remains challenging. Here we report unexpected, large mass-independent sulphur isotopic compositions of pyrite with Δ33S of up to 0.91‰ in Late Ordovician sedimentary rocks from South China. The magnitude of the Δ33S is similar to that discovered in ice core sulphate originating from stratospheric volcanism. The coincidence between the large Δ33S and the first pulse of the Late Ordovician mass extinction about 445 million years ago suggests that stratospheric volcanic eruptions may have contributed to synergetic environmental deteriorations such as prolonged climatic perturbations and oceanic anoxia, related to the mass extinction.
Machine-learning-revealed statistics of the particle-carbon/binder detachment in lithium-ion battery cathodes
Authors: Jiang Zhisen , Li Jizhou , Yang Yang , Mu Linqin , Wei Chenxi , Yu Xiqian , Pianetta Piero , Zhao Kejie , Cloetens Peter , Lin Feng , Liu Yijin 
Published: May 2020

AbstractThe microstructure of a composite electrode determines how individual battery particles are charged and discharged in a lithium-ion battery. It is a frontier challenge to experimentally visualize and, subsequently, to understand the electrochemical consequences of battery particles’ evolving (de)attachment with the conductive matrix. Herein, we tackle this issue with a unique combination of multiscale experimental approaches, machine-learning-assisted statistical analysis, and experiment-informed mathematical modeling. Our results suggest that the degree of particle detachment is positively correlated with the charging rate and that smaller particles exhibit a higher degree of uncertainty in their detachment from the carbon/binder matrix. We further explore the feasibility and limitation of utilizing the reconstructed electron density as a proxy for the state-of-charge. Our findings highlight the importance of precisely quantifying the evolving nature of the battery electrode’s microstructure with statistical confidence, which is a key to maximize the utility of active particles towards higher battery capacity.
Interface enhanced functionalities in oxide superlattices under mechanical and electric boundary conditions
Authors: Wang Hongwei , Tang Fujie , Dhuvad Pratikkumar H. , Wu Xifan 
Published: May 2020

AbstractIn recent years, the inverse design of artificial materials, in the format of thin-films and superlattices, has been an active sub-field in material science. From a joint effort from both experiment and theory, scientists are searching for new engineering methods or design rules so that the materials can be custom designed with desired functionalities in theory before the materials are actually synthesized by epitaxial growth technique in laboratory. In this article, we provide a short summary of the recently proposed epitaxial strain and interface design approaches for the functional artificial oxide heterostructures. The underlying physical mechanism enabling the enhanced functional properties, such as ferroelectricity and multiferroics, are briefly reviewed. In particular, focused discussions are made on the proper treatments of both mechanical and electric boundary conditions when the oxide thin-films and superlattices are theoretically modeled by first-principles computer simulations.
Transcript profiling provides insights into molecular processes during shoot elongation in temperature-sensitive peach (Prunus persica)
Authors: Lian Xiaodong , Tan Bin , Yan Liu , Jiang Chao , Cheng Jun , Zheng Xianbo , Wang Wei , Chen Tanxing , Ye Xia , Li Jidong , Feng Jiancan 
Published: May 2020

AbstractPlant growth caused by ambient temperature is thought to be regulated by a complex transcriptional network. A temperature-sensitive peach (Prunus persica) was used to explore the mechanisms behind shoot internode elongation at elevated temperatures. There was a significantly positive correlation between the length of the terminal internode (TIL) and the maximum temperature three days prior to the measuring day. Four critical growth stages (initial period and initial elongation period at lower temperature, rapid growth period and stable growth period at higher temperature) were selected for comparative RNA-seq analysis. About 6.64G clean bases were obtained for each library, and 88.27% of the data were mapped to the reference genome. Differentially expressed gene (DEG) analysis among the three pairwise comparisons resulted in the detection of several genes related to the shoot elongation in temperature-sensitive peach. HSFAs were up-regulated in response to the elevated temperature, while the up-regulated expression of HSPs might influence hormone signaling pathways. Most of DEGs involved in auxin, abscisic acid and jasmonic acid were up-regulated, while some involved in cytokinin and brassinosteroid were down-regulated. Genes related to ethylene, salicylic acid and circadian rhythm were also differentially expressed. Genes related to aquaporins, expansins, pectinesterases and endoglucanase were up-regulated, which would promote cell elongation. These results lay a foundation for further dissection of the regulatory mechanisms underlying shoot elongation at elevated temperatures.
The relation of Bleomycin Delivery Efficiency to Microbubble Sonodestruction and Cavitation Spectral Characteristics
Authors: Maciulevičius Martynas , Tamošiūnas Mindaugas , Venslauskas Mindaugas S. , Šatkauskas Saulius 
Published: May 2020

AbstractThe concurrent assessment of principal sonoporation factors has been accomplished in a single systemic study. Microbubble sonodestruction dynamics and cavitation spectral characteristics, ultrasound scattering and attenuation, were examined in relation to the intracellular delivery of anticancer drug, bleomycin. Experiments were conducted on Chinese hamster ovary cells coadministered with Sonovue microbubbles. Detailed analysis of the scattering and attenuation temporal functions culminated in quantification of metrics, inertial cavitation dose and attenuation rate, suitable for cavitation control. The exponents, representing microbubble sonodestruction kinetics were exploited to derive dosimetric, microbubble sonodestruction rate. High intracorrelation between empirically-attained metrics defines the relations which indicate deep physical interdependencies within inherent phenomena. Subsequently each quantified metric was validated to be well-applicable to prognosticate the efficacy of bleomycin delivery and cell viability, as indicated by strong overall correlation (R2 > 0.85). Presented results draw valuable insights in sonoporation dosimetry and contribute towards the development of universal sonoporation dosimetry model. Both bleomycin delivery and cell viability reach their respective plateau levels by the time, required to attain total microbubble sonodestruction, which accord with scattering and attenuation decrease to background levels. This suggests a well-defined criterion, feasible through signal-registration, universally employable to set optimal duration of exposure for efficient sonoporation outcome.
Disorder by design: A data-driven approach to amorphous semiconductors without total-energy functionals
Authors: Limbu Dil K. , Elliott Stephen R. , Atta-Fynn Raymond , Biswas Parthapratim 
Published: May 2020

AbstractX-ray diffraction, Amorphous silicon, Multi-objective optimization, Monte Carlo methods. This paper addresses a difficult inverse problem that involves the reconstruction of a three-dimensional model of tetrahedral amorphous semiconductors via inversion of diffraction data. By posing the material-structure determination as a multiobjective optimization program, it has been shown that the problem can be solved accurately using a few structural constraints, but no total-energy functionals/forces, which describe the local chemistry of amorphous networks. The approach yields highly realistic models of amorphous silicon, with no or only a few coordination defects (≤1%), a narrow bond-angle distribution of width 9–11.5°, and an electronic gap of 0.8–1.4 eV. These data-driven information-based models have been found to produce electronic and vibrational properties of a-Si that match accurately with experimental data and rival that of the Wooten-Winer-Weaire models. The study confirms the effectiveness of a multiobjective optimization approach to the structural determination of complex materials, and resolves a long-standing dispute concerning the uniqueness of a model of tetrahedral amorphous semiconductors obtained via inversion of diffraction data.
Biochemical characteristics of the chondrocyte-enriched SNORC protein and its transcriptional regulation by SOX9
Authors: Jaiswal Prashant Kumar , Aljebali Latifa , Gaumond Marie-Hélène , Oh Chun-do , Yasuda Hideyo , Moffatt Pierre 
Published: May 2020

AbstractSnorc (Small NOvel Rich in Cartilage) has been identified as a chondrocyte-specific gene in the mouse. Yet little is known about the SNORC protein biochemical properties, and mechanistically how the gene is regulated transcriptionally in a tissue-specific manner. The goals of the present study were to shed light on those important aspects. The chondrocyte nature of Snorc expression was confirmed in mouse and rat tissues, in differentiated (day 7) ATDC5, and in RCS cells where it was constitutive. Topological mapping and biochemical analysis brought experimental evidences that SNORC is a type I protein carrying a chondroitin sulfate (CS) attached to serine 44. The anomalous migration of SNORC on SDS-PAGE was due to its primary polypeptide features, suggesting no additional post-translational modifications apart from the CS glycosaminoglycan. A highly conserved SOX9-binding enhancer located in intron 1 was necessary to drive transcription of Snorc in the mouse, rat, and human. The enhancer was active independently of orientation and whether located in a heterologous promoter or intron. Crispr-mediated inactivation of the enhancer in RCS cells caused reduction of Snorc. Transgenic mice carrying the intronic multimerized enhancer drove high expression of a βGeo reporter in chondrocytes, but not in the hypertrophic zone. Altogether these data confirmed the chondrocyte-specific nature of Snorc and revealed dependency on the intronic enhancer binding of SOX9 for transcription.
Incidental learning of group trust: Predictive gaze cue matters
Authors: Sun Zhongqiang , He Zhihui , Zhang Guochao , Li Xinyu , Yu Wenjun 
Published: May 2020

AbstractHuman gaze is a subtle cue to deliver information and helps impression formation in social interactions. People automatically follow the gaze direction of others and shift their attention accordingly, as well as determine the trustworthiness of others based on the predictable validity of their gaze behavior, yet it remains unclear how this works at the collective level. Therefore, the current study is the first to explore the incidental learning of trust from a group’s gaze behavior. To simulate different patterns of perceiving collective information in real life, two ways of presenting group member gazes were used in the object categorization task, the simultaneous way in Experiment 1 and the sequential way in Experiment 3, and a sampling strategy was ruled out in Experiment 2. Converging findings in experiments demonstrated a typical gaze-cueing effect, and more importantly, the Predictive-valid group obtained more trust compared to the Predictive-invalid group. To enrich and expand the applicability of the incidental trust learning effect from gazes, the current study provides supportive evidence at the collective level, confirming that humans have an efficient capability to process gaze information of groups.
The pivot point arginines identified in the β-pinwheel structure of C-terminal domain from Salmonella Typhi DNA Gyrase A subunit
Authors: Sachdeva Ekta , Kaur Gurpreet , Tiwari Pragya , Gupta Deepali , Singh Tej P. , Ethayathulla Abdul S. , Kaur Punit 
Published: May 2020

AbstractThe essentiality of DNA Gyrase in basic cellular processes in bacterial pathogens makes it an ideal drug target. Though the Gyrase has a conserved mechanism of action, the complete DNA wrapping and binding process is still unknown. In this study, we have identified six arginine residues R556, R612, R667, R716, R766, and R817 in the DNA GyraseA – C-terminal domain from Salmonella enterica serovar Typhi (StGyrA-CTD) to be essential for DNA wrapping and sliding by a sequence and structure analysis. Through site-directed mutagenesis and EMSA studies, we observed that the substitution of R667 (blade 3) and R716 (blade 4) in StGyrA-CTD led to loss of DNA binding. Whereas, upon mutation of residue R612 (blade2), R766 (blade5) and R817 (blade6) along with supporting residue R712 (blade 4) a decrease in binding affinity was seen. Our results indicate that R667 and R716 act as a pivot point in DNA wrapping and sliding during gyrase catalytic activity. In this study, we propose that the DNA wrapping mechanism commences with DNA binding at blade3 and blade4 followed by other blades to facilitate the DNA sliding during supercoiling activity. This study provides a better understanding of the DNA binding and wrapping mechanism of GyrA-CTD in DNA Gyrase.
Body-size Scaling is Related to Gut Microbial Diversity, Metabolism and Dietary Niche of Arboreal Folivorous Flying Squirrels
Authors: Liu Po-Yu , Cheng An-Chi , Huang Shiao-Wei , Lu Hsiao-Pei , Oshida Tatsuo , Liu Wenhua , Yu Hon-Tsen 
Published: May 2020

AbstractThermal homeostasis of mammals is constrained by body-size scaling. Consequently, small mammals require considerable energy to maintain a high mass-specific metabolic rate (MSMR) and sustain target body temperature. In association with gut microbiota, mammalian hosts acquire absorbable molecules and fulfill their metabolic requirements. Our objective was to characterize gut microbes in wild mammals and relate those findings to host body-size scaling. Two large (Petaurista philippensis grandis and P. alborufus lena), one medium (Trogopterus xanthipes) and one small (Pteromys volans orii) species of flying squirrels (FS) were studied. Using 16S rRNA genes, 1,104 OTUs were detected from four FS, with 1.99% of OTUs shared among all FS. Although all FS gut microbiota were dominated by Firmicutes, they were constituted by different bacterial families. Moreover, Bacteroidetes accounted for up to 19% of gut microbiota in small FS, but was absent in large FS. Finally, based on metagenome predictions, carbohydrate and amino acid metabolism genes were enriched in small body-size FS. In conclusion, gut microbiota compositions and predictive metabolic functions were characteristic of body-size in FS, consistent with their adaptations to folivorous dietary niches.
Local biases drive, but do not determine, the perception of illusory trajectories
Authors: Gheorghes Tamara N. , Richardson Paul , Reidy John 
Published: May 2020

AbstractWhen a dot moves horizontally across a set of tilted lines of alternating orientations, the dot appears to be moving up and down along its trajectory. This perceptual phenomenon, known as the slalom illusion, reveals a mismatch between the veridical motion signals and the subjective percept of the motion trajectory, which has not been comprehensively explained. In the present study, we investigated the empirical boundaries of the slalom illusion using psychophysical methods. The phenomenon was found to occur both under conditions of smooth pursuit eye movements and constant fixation, and to be consistently amplified by intermittently occluding the dot trajectory. When the motion direction of the dot was not constant, however, the stimulus display did not elicit the expected illusory percept. These findings confirm that a local bias towards perpendicularity at the intersection points between the dot trajectory and the tilted lines cause the illusion, but also highlight that higher-level cortical processes are involved in interpreting and amplifying the biased local motion signals into a global illusion of trajectory perception.
Repeated evolution of durophagy during ichthyosaur radiation after mass extinction indicated by hidden dentition
Authors: Huang Jian-dong , Motani Ryosuke , Jiang Da-yong , Ren Xin-xin , Tintori Andrea , Rieppel Olivier , Zhou Min , Hu Yuan-chao , Zhang Rong 
Published: May 2020

AbstractMarine tetrapods quickly diversified and were established as marine top predators after the end-Permian Mass extinction (EPME). Ichthyosaurs were the forerunner of this rapid radiation but the main drivers of the diversification are poorly understood. Cartorhynchus lenticarpus is a basal ichthyosauriform with the least degree of aquatic adaptation, holding a key to identifying such a driver. The unique specimen appeared edentulous based on what was exposed but a CT scanning revealed that the species indeed had rounded teeth that are nearly perpendicular to the jaw rami, and thus completely concealed in lateral view. There are three dental rows per jaw ramus, and the root lacks infoldings of the dentine typical of ichthyopterygians. The well-developed and worn molariform dentition with three tooth rows supports the previous inference that the specimen is not of a juvenile. The premaxilla and the corresponding part of the dentary are edentulous. Molariform dentition evolved three to five times independently within Ichthyosauriformes in the Early and Middle Triassic. Convergent exploitation of hard-shelled invertebrates by different subclades of ichthyosauriforms likely fueled the rapid taxonomic diversification of the group after EPME.
Proteomic analysis upon peach fruit infection with Monilinia fructicola and M. laxa identify responses contributing to brown rot resistance
Authors: Papavasileiou Antonios , Tanou Georgia , Samaras Anastasios , Samiotaki Martina , Molassiotis Athanassios , Karaoglanidis George 
Published: May 2020

AbstractBrown rot, caused by Monilinia spp., is a major peach disease worldwide. In this study, the response of peach cultivars Royal Glory (RG) and Rich Lady (RL) to infection by Monilinia fructicola or Monilinia laxa, was characterized. Phenotypic data, after artificial inoculations, revealed that ‘RL’ was relatively susceptible whereas ‘RG’ was moderately resistant to Monilinia spp. Comparative proteomic analysis identified mesocarp proteins of the 2 cultivars whose accumulation were altered by the 2 Monilinia species. Functional analysis indicated that pathogen-affected proteins in ‘RG’ were mainly involved in energy and metabolism, while, differentially accumulated proteins by the pathogen presence in ‘RL’ were involved in disease/defense and metabolism. A higher number of proteins was differentiated in ‘RG’ fruit compared to ‘RL’. Upon Monilinia spp. infection, various proteins were-down accumulated in ‘RL’ fruit. Protein identification by mass spectrometric analysis revealed that several defense-related proteins including thaumatin, formate dehydrogenase, S-formylglutathione hydrolase, CBS domain-containing protein, HSP70, and glutathione S-transferase were up-accumulated in ‘RG’ fruit following inoculation. The expression profile of selected defense-related genes, such as major latex allergen, 1-aminocyclopropane-1-carboxylate deaminase and UDP-glycoltransferase was assessed by RT-PCR. This is the first study deciphering differential regulations of peach fruit proteome upon Monilinia infection elucidating resistance responses.
Strain-induced structure and oxygen transport interactions in epitaxial La0.6Sr0.4CoO3−δ thin films
Authors: Ivanov Yurii P. , Kubicek Markus , Siebenhofer Matthäus , Viernstein Alexander , Hutter Herbert , Fleig Jürgen , Chuvilin Andrey , Zhang Zaoli 
Published: May 2020

AbstractThe possibility to control oxygen transport in one of the most promising solid oxide fuel cell cathode materials, La0.6Sr0.4CoO3−δ, by controlling lattice strain raises questions regarding the contribution of atomic scale effects. Here, high-resolution transmission electron microscopy revealed the different atomic structures in La0.6Sr0.4CoO3−δ thin films grown under tensile and compressive strain conditions. The atomic structure of the tensile-strained film indicated significant local concentration of the oxygen vacancies, with the average value of the oxygen non-stoichiometry being much larger than for the compressive-strained film. In addition to the vacancy concentration differences that are measured by isotope exchange depth profiling, significant vacancy ordering was found in tensile-strained films. This understanding might be useful for tuning the atomic structure of La0.6Sr0.4CoO3−δ thin films to optimize cathode performance.