bluuebunny/crossref_metadata_2025_split huggingface dataset:
Molecular characterization and serodiagnostic potential of two serpin proteins in Psoroptes ovis var. cuniculi
Authors: Gu Xiaobin , Chen Yuhang , Zhang Chongyang , Xie Yue , Shen Nengxing , Wang Ce , Zhou Xuan , Yang Guangyou , He Ran , Peng Xuerong , Yang Deying , He Zhi , Zhong Zhijun 
Published: December 2020

Abstract
Background: Psoroptes ovis var. cuniculi is a global common ectoparasite of wild and domestic rabbits and causes an economically devastating loss and serious welfare issues of commercial rabbit husbandry. Serine proteinase inhibitor (serpin) is present in almost all organisms that are involved in host-pathogen interactions, inflammatory responses, and reproductive development, etc. However, the research on P. ovis var. cuniculi serpins is still limited.Methods: In this study, two serpin genes of P. ovis var. cuniculi (Pso c 27 and PsoSP2 cDNAs) were cloned, and the molecular characterization was analyzed. The transcriptional profiles and tissue localization of these two serpins in P. ovis var. cuniculi were investigated by quantitative real-time PCR and immunohistochemistry, respectively. The potential function of recombinant Pso c 27 and PsoSP2 (rPso c 27and rPsoSP2) in the serodiagnosis of P. ovis var. cuniculi infestation in rabbits were evaluated by indirect enzyme-linked immunosorbent assay (iELISA). Results: Both of the 523 residue Pso c 27 and the 240 residue PsoSP2 proteins contained typical serpin domains and signatures. Both Pso c 27and PsoSP2 cDNAs expressed throughout the life-cycle, more specifically, significantly higher expression in female mites than the larva, nymph, and male mites (Pso c 27, F(3, 8) = 1935.953, P < 0.0001; PsoSP2, F(3, 8) = 660.669, P < 0.0001). The native Pso c 27 and PsoSP2 localized in ovary and mouthpart of adult female mites, respectively. Compared to rPsoSP2, the rPso c 27 displayed better diagnostic efficiency with higher values of sensitivity, specificity and the area under the receiver operating characteristic curve (AUC) (rPso c 27 vs rPsoSP2: 96.0 vs 90.0%; 90.91 vs 78.18%; 0.988 vs 0.964, respectively). Moreover, the rPso c 27 showed seropositive in 80% rabbits as early as the 2 weeks post-infestation (p.i.), prior to visible clinical signs and microscopy-positive of skin scrapings. Conclusions: These results suggested that these two serpins may play essential roles in reproductive development, serum-feeding, and pathogenicity of P. ovis var. cuniculi. Compared to PsoSP2, Pso c 27 appeared as a potential antigen for serodiagnosis of P. ovis var. cuniculi infestation in rabbits, especially at the early stage of infestation.
Expanding enhanced recovery protocols for cardiac surgery to include the patient voice: A scoping review protocol
Authors: Oravec Nebojša , Arora Rakesh C. , Bjorklund Brian , Gregora April , Monnin Caroline , Duhamel Todd A. , Kent David E. , Schultz Annette S.H. , Chudyk Anna Maria 
Published: December 2020

Abstract
BackgroundCardiac surgery is becoming increasingly common in older, more vulnerable adults. A focus on timely and complete medical and functional recovery has led to the development of enhanced recovery protocols (ERPs) for a number of surgical procedures and subspecialties, including cardiac surgery (ERAS® Cardiac). An element that is often overlooked in the development and implementation of ERPs is the involvement of key stakeholder groups, including surgery patients and caregivers (e.g., family and/or friends). The aim of this study is to describe a protocol for a scoping review of cardiac patient and caregiver preferences and outcomes relevant to cardiac surgery ERPs. MethodsUsing Arskey and O’Malley’s six-stage framework for scoping review methodologies with adaptions from Levac et al., a scoping review of existing literature describing patient- and caregiver-identified preferences and outcomes as they relate to care received in the perioperative period of cardiac surgery will be undertaken. The search for relevant articles will be conducted using electronic databases (i.e., the Cochrane Library, Medline, PsycINFO, Scopus, and Embase), as well as through a search of the grey literature (e.g., CPG Infobase, Heart and Stroke Foundation, ProQuest Theses and Dissertations, Google Advanced and Prospero). Published and unpublished full-text articles written in English, published after the year 2000, and that relate to the research question will be included. Central to the design of this scoping review is our collaboration with two patient partners who possess lived experience as cardiac surgery patients.DiscussionThis review will identify strategies that can be integrated into ERPs for cardiac surgery which align with patient- and caregiver-defined values. Broadly, it is our goal to demonstrate the added value of patient engagement in research to aid in the success of system change processes.
Morphological characterization and Genetic diversity analysis of a Tunisian durum wheat (Triticum turgidum var. durum) collection
Authors: Ouaja Maroua , Bahri Bochra Amina , Aouini Lamia , Ferjaoui Sahbi , Medini Maher , Marcel Thierry , Hamza Sonia Mihed 
Published: December 2020

Abstract
Background: Tunisia is a center of genetic diversity of durum wheat and has a large number of abandoned old local landraces. An accurate investigation and characterization of the morphological and genetic features of these landraces would allow their rehabilitation and use for practical and beneficial purposes. In this context, a collection of 304 local accessions of durum wheat, collected from five regions and three climatic zones of central and southern Tunisia, was studied. Results: Morphological characterization was carried out using 12 spike-related traits and rendered a mean Shannon-Weaver Index (H') of 0.80 indicating the presence of a high level of polymorphism among accessions. Based on these traits 11 local landraces, namely Mahmoudi, Azizi, Jneh Khotifa, Mekki, Biskri, Taganrog, Biada, Badri, Richi, Roussia and Souri were identified. Spike length (H’=0.98) and shape (H’=0.86) with grains size (H’=0.94), form (H’=0.87) and color (H’=0.86) were the most polymorphic morphological traits. The genetic diversity was assessed using 10 SSR markers, with a polymorphic information content (PIC) of 0.69. Levels of genetic diversity were generally high, with a Shannon's Information Index (I) of 0.62 and a gene diversity (He) of 0.35. In addition, population structure analysis distinguished 11 genetic subpopulations significantly correlated with the morphological identification. Analysis of molecular variance (AMOVA) showed high genetic variations within regions (81%) and within wheat subpopulations (41%) reflecting a considerable amount of admixture between landraces. The moderate (19%) and high (59%) genetic variations among regions and among wheat subpopulations observed highlighted farmers selection practices . Furthermore, Mahmoudi landrace showed spike densities significantly different between the center to the south of Tunisia; notably loose spikes with open glumes in the south and compact ones in the center, which may represent an adaptation form for tolerance to high temperature. Conclusion: Overall, this study underlined the genetic richness of local resources for better in situ or ex situ conservation and for their subsequent use in plant breeding programs.
Effect of novel coronavirus disease 2019 infection on chronic kidney disease G1-G5, G5 Dialysis and G5 Transplantation
Authors: Amiri Fateme Shamekhi 
Published: December 2020

Abstract
Background: The pneumonia caused by the 2019 novel coronavirus (SARS-CoV-2) is a highly infectious disease that causes lethal disease and multiorgan failure. The aim of this research is to investigate association between coronavirus disease 2019 (COVID-19) infection and kidney dysfunction.Methods and Materials: In this retrospective study, sixty-eight patients with kidney dysfunction and COVID-19 infection were investigated. Clinical features, laboratory data at initial presentation, management and outcomes were collected.The paper has written based on searching PubMed Central and Google Scholar to identify potentially relevant articles. Median, percentage, mean ± standard deviation (SD), two-tailed t and chi-square and Cohen᾽s-d tests were used for statistical analyses. Moreover, relative risk, odds ratio, pearson᾽s correlation for statistical analyses were used.Results: The average age of patients at time of diagnosis in COVID-19 nephropathy was 52.04 ± 14.42 years (ranging from 24 years to 88 years). There was not statistical significance correlation between lymphocytopenia and serum creatinine (SCr) in COVID-19 nephropathy (R2=0.063; p-value= 0.33). Effect size of elevated IL-6 on decreased estimated glomerular filtration rate (eGFR) in COVID-19 nephropathy was assessed 0.656 (medium effect size). Relative risk and odds ratio of acute kidney disease (AKD) in COVID-19 nephropathy were assessed 0.57 and 0.4, respectively (p-value: 0.422). Correlation between SCr changes and time of emergent acute kidney injury (AKI), AKD and chronic kidney disease (CKD) was assessed with R2 of 0.0003 and p-value of 0.94 (not significant). Conclusion: The present study revealed medium effect size of elevated IL-6 on decreased eGFR. Future clinical research is required for investigating novel unknown findings in COVID-19 nephropathy.
Abnormal Eye Movements in Spinocerebellar Ataxia Type 3
Authors: Lin Junyu , Zhang Lingyu , Cao Bei , Wei Qianqian , Ou Ruwei , Hou Yanbing , Xu Xinran , Liu Kuncheng , Gu Xiaojing , Shang Huifang 
Published: December 2020

Abstract
Background: Abnormal eye movements are common in spinocerebellar ataxias Type 3 (SCA3). We conducted the research to explore the frequency of abnormal eye movements in Chinese patients with SCA3, to compare the demographic and clinical characteristics between SCA3 patients with and without each type of abnormal eye movement, and to explore the correlation between abnormal eye movements and the severity of ataxia.Methods: Seventy-four patients with SCA3 were enrolled in this cross-sectional study. Six types of abnormal eye movements including impaired smooth pursuit, increased square-wave jerks (SWJ), gaze-evoked nystagmus (GEN), slowing of saccades, saccadic hypo/hypermetria and supranuclear gaze palsy were evaluated by experienced neurologists. The severity of ataxia was evaluated by Scale for the Assessment and Rating of Ataxia (SARA). Results: The prevalence of impaired smooth pursuit, increased SWJ, GEN, slowing of saccades, saccadic hypo/hypermetria and supranuclear gaze palsy in Chinese SCA3 patients was 28.4%, 13.5%, 78.4%, 41.9%, 23.0%, and 5.4%, respectively. SCA3 patients with GEN had higher scores of International Cooperative Ataxia Rating Scale (ICARS-IV) and total ICARS, and longer length of CAG repeat than patients without GEN. SCA3 patients with slowing of saccades had a longer disease duration, higher scores of ICARS-I, ICARS-II, total ICARS and SARA than patients without slowing of saccades. SCA3 patients with saccadic hypo/hypermetria had higher scores of ICARS-III, ICARS-IV, and SARA than patients without saccadic hypo/hypermetria. The demographic and clinical characteristics did not differ significantly between SCA3 patients with and without impaired smooth pursuit, increased SWJ, or supranuclear gaze palsy. Multivariate linear regression showed that the number of abnormal eye movements (0-6), disease duration, Hamilton Depression Rating Scale-24 (HDRS-24) score, and CAG repeat length were positively correlated with SARA score, whereas Montreal Cognitive Assessment (MoCA) score was negatively correlated with SARA score in SCA3. Conclusions: An increased number of abnormal eye movement types correlated with the severity of ataxia in SCA3.
Czy istnieje sezonowy rytm rozwoju w warunkach tropikalnych? (Raport 1 z dwuletnich comiesięcznych badań na Jukatanie w Meksyku)
Authors: Siniarska Anna , Rojas Armando , Valentin Graciela , Wolański Napoleon , Dickinson Federico 
Published: December 2020

The main aim of this work concerns the answer a question whether the general regularity of seasonal differences in changes of height, weight, and body components during ontogeny in tropics exist. If so, whether this phenomenon depends on cyclic changes in nature or this rather is an adjustment to local conditions and mode of life. To answer this question the studies took place in the tropical climate of Yucatan and included 49 boys and 47 girls aged 11-12 years and being of the Maya, Mestizo, and Creole origin. The youths were attending two schools which were located in rather poor districts of Merida (the capital city of the Yucatan State, Mexico). The investigations started in February 2002 and ended in November 2003 and were continued monthly. The standard anthropometric methodology was applied to measure body height, weight, arm, waist, hip and calf circumferences, and five subcutaneous fat folds (biceps and triceps brachii, subscapular, suprailiac and calf). Bioimpedance techniques were used to estimate fat mass (FM ), fat-free mass (FFM ), and total body water (TBW). The results show that general regularities in monthly or longer rates of stature increments and increments or declines of body mass do not exist. There are not similar regularities of changes even in groups of coevals of the same gender and within the youths coming from the same district. Each variable shows a quite specific rate of changes.
Coronavirus (COVID-19) and Employee Health
Authors: Tavana Ali Mehrabi , Bahadori Mohamad Karim 
Published: December 2020

Background::
The coronavirus, which is now spreading around the world as a pandemic, has caused more than 30
million cases and nearly 1000,000 deaths worldwide. No vaccine has been used against the virus so far and there are no specific
drugs to cure the patients. This paper examines the impact of coronavirus COVID-19 pandemic on employees’ health,
either in governmental or private sector. The aim of this study was to protect staffs against disease in pandemic conditions
by identifying the main ways to keep employee healthy during COVID-19 pandemic in different scenarios.

Methods::
This paper is a perspective article and different scenario were assessed in order for consideration of employee
health.

Results::
Two scenarios could be considered first, when, the vaccine against Covid-19 is not available for prevention and the
second scenario is that the vaccine is available for all in the world. In both cases, maintaining the health of the staff will be
different. If the coronavirus disease will continue to occur in humans as long as access to a safe vaccine is not possible, emphasis
on health standards, social distance and wearing the mask is the only way to deal with this dangerous disease. Even,
with preparation of vaccine the health standards, social distancing, and wearing of mask must be continued in any situations.

Conclusion::
The high level of health for employee must be considered by governments in different scenarios.
Les “Réfugiés clandestins”: hommes ou animaux?
Authors: Flores Maya Agustín 
Published: December 2020

Dans les principaux pays européens, les militants de la « cause animale » réexaminent la terminologie juridique, politique et philosophique relative aux animaux. Ils s’attaquent, en particulier, au caractère anthropocentriste de certaines notions justifiant et prolongeant l’exclusion des animaux de la sphère de considération morale. Tandis qu’on assiste à la revendication des vies animales, dans ces mêmes pays, l’absence de reconnaissance sociale et d’une protection juridique condamne les migrants au nomadisme et à la clandestinité. Ce travail souhaite démontrer comment l’absence des droits fondamentaux chez les « réfugiés clandestins » rabaisse l’homme à l’état animal. Un rabaissement démontrant que la vulnérabilité des vies humaines et des vies animales est au-delà des espèces. Un élément essentiel pour construire une nouvelle éthique considérant comme égaux les hommes et les animaux, une éthique humanimaliste.
Liberdade e determinismo Pós-Humano
Authors: Rocha Guilherme Massara , Da Silva Vanessa Guimarães 
Published: December 2020

O presente artigo examina processos, circunstâncias e contextos da obra da artista francesa contemporânea ORLAN. Privilegia-se aqui a hipótese de tratar-se de uma estética que se desenvolve a partir de matrizes convergentes com aquelas que forjam substancialmente o discurso pós-humanístico. Matrizes que, por um lado, se divorciam da normatividade em jogo na moralidade racional pós-humanística, mas que paradoxalmente a ela parecem se subordinar. Buscaremos compreender em que medida a força emancipatória de uma obra que critica as normas de gênero e identidade formais e estéticas se conecta, num certo momento, a um ideal edênico de pureza da corporeidade abstrata e bem-aventurada.
A distopia em tempos de pandemia
Authors: De Camargo Santos Taynnã 
Published: December 2020

No âmbito dos saraus temáticos online promovidos pelo coletivo Sinestéticas, a edição de 13 de maio de 2020 trouxe à pauta as Distopias. Sob a égide de uma pandemia global marcada por conflitos políticos e sociais em diversos países, observamos o retorno às listas dos livros mais vendidos de alguns clássicos distópicos como 1984, de George Orwell, Ensaio Sobre a Cegueira, de José Saramago e O Conto de Aia, de Margaret Atwood.
A escritora e tradutora brasileira Luisa Geisler, duas vezes ganhadora do Prêmio Sesc de Literatura e finalista do prêmio Jabuti, falou sobre a escrita coletiva e o lançamento em plena pandemia da distopia Corpos Secos (2020). Esta entrevista, conduzida por Taynnã de Camargo Santos, foi transcrita e expandida para publicação na edição nº 2 da Revista 2i.
ADHERENCE TO IRON SUPPLEMENTATION INFLUENCES ANEMIA IN PREGNANCY
Authors: Kusumawardani Nurul 
Published: December 2020

<p><em>Women with pregnancy have a higher risk of developing anemia with iron deficiency.</em><em> </em><em>This condition is due to the maternal body requirements for a significant iron, but the intake and storage of iron in the body are inadequate.</em><em> </em><em>The aim of this study was to describe the compliance of pregnant women in consuming iron supplements and correlation with anemia in pregnancy.</em><em> </em><em>This purpose of the study to describe the compliance of pregnant women in consuming iron supplements and their relationship with the incidence of anemia in pregnancy. </em><em>This study is a descriptive correlation study with a case-control design.</em><em> </em><em>The sampling technique used was non-probability sampling with the consecutive sampling method, so that 68 respondents of pregnancy in their third trimester who routinely performed antenatal care (ANC) since the beginning of pregnancy at Puskesmas Jetis, Yogyakarta City in February 2019.</em><em> Chi-square statistical analysis (X</em><em><sup>2</sup></em><em> test) with Spearman's rank correlation coefficient (<span style="font-family: Arial;">ρ</span><span style="font-family: Calibri;">) to determine the strength and direction of the correlation between anemia and respondent compliance based on the pill count method. </span></em><em>The results showed that respondents who did not adhere with iron supplements had a significant relationship with the incidence of anemia (X</em><em><sup>2</sup></em><em> = 11.56; p-value= 0.001 (p</em><em>&lt;</em><em>0.050)), odds ratio (OR) = 10.79; 95% CI 2.25-51.66 and has a positive correlation direction with moderate strength (ρ-value= 0.412, p</em><em>-value</em><em> = 0.001), so it can be concluded that pregnant women who are not adherent will have 10 times the risk of experiencing anemia (Hb 11.5 g/dL) during pregnancy.</em></p>
Время приема блокаторов рецепторов ангиотензина II и их антигипертензивный эффект: собственный опыт хронотерапии
Authors: Rekovets O.L. , Torbas O.O. , Kushnir S.M. , Primak G.F. , Granich V.M. , Sidorenko P.I. , Polischuk S.A. , Ponomareva G.V. , Radchenko G.D. , Zhivilo I.O. , Sirenko O.Yu. , Krushynska N.A. , Sirenko Yu.M. 
Published: December 2020

Целью нашего исследования было оценить влияние блокаторов рецепторов к ангиотензину II (АТ II) — олмесартана, азилсартана и телмисартана при приеме их в утренние или вечерние часы на показатели артериального давления (АД) при суточном мониторировании. Материалы и методы. В исследование было включено 126 пациентов с мягкой и умеренной артериальной гипертензией, которые были отобраны для сравнения влияния на уровень АД медикаментозной хронотерапии блокаторами рецепторов к АТ II — олмесартаном, азилсартаном и телмисартаном при приеме их в утренние или вечерние часы и разделены на 6 подгрупп: 1-я — 20 пациентов, принимавших олмесартан 20–40 мг в утренние часы, 2-я — 20 пациентов, принимавших олмесартан 20–40 мг в вечерние часы, 3-я — 21 пациент, принимавшие азилсартан 40–80 мг в утренние часы, 4-я — 20 пациентов, принимавших азилсартан 40–80 мг в вечерние часы, 5-я — 22 пациента, принимавшие телмисартан 40–80 мг в утренние часы, 6-я — 23 пациента, принимавшие телмисартан 40–80 мг в вечерние часы. Пациенты проходили первичное и повторное обследование через 3 месяца терапии. Результаты. Вечерний прием олмесартана приводил к более выраженному снижению среднесуточного систолического АД (24САД) — 11,09 ± 2,30 мм рт.ст. против 4,06 ± 2,25 мм рт.ст., р < 0,01. Изменения среднесуточного диастолического АД (24ДАД) были недостоверными, хотя снижение при приеме в вечерние часы было более значимым по сравнению с утренним приемом — 8,38 ± 2,58 мм рт.ст. против 3,38 ± 2,31 мм рт.ст., НД. Изменения 24САД/24ДАД на фоне приема азилсартана в вечерние или утренние часы были достоверными, но не отличались между собой — 13,06 ± 2,65/9,76 ± 1,73 мм рт.ст. и 12,71 ± 1,62/7,00 ± ± 1,50 мм рт.ст., НД. Снижение 24САД/24ДАД на фоне приема телмисартана было достоверно более выраженное при утреннем приеме по сравнению с вечерним приемом — 16,48 ± 2,86/12,56 ± 2,80 мм рт.ст. против 4,93 ± 1,53/5,40 ± ± 1,89 мм рт.ст., р < 0,01. Таким образом, утренний прием более выражено снижал среднесуточный АД на фоне приема телмисартана, а вечерний прием — на фоне приема олмесартана. Азилсартан одинаково снижал АД при приеме как в вечерние часы, так и в утренние. Достижение целевого АД при суточном мониторировании на фоне приема олмесартана, азилсартана и телмисартана составило 71,80; 71,05 и 75,61 % соответственно. Выводы. Исследование различных блокаторов АТ II (олмесартана, азилсартана и телмисартана) продемонстрировало их различные свойства по воздействию на суточный уровень АД в зависимости от времени приема. Мы не выявили классового эффекта препаратов по времени их приема: олмесартан был более эффективным при приеме в вечерние часы, телмисартан — в утренние, а эффективность азилсартана не зависела от времени его приема. Достижение целевого АД на фоне приема всех трех препаратов по данным суточного мониторирования было одинаковым.