bluuebunny/crossref_metadata_2025_split huggingface dataset:
Initial pig developmental stage influences intestinal organoid growth but not cellular composition
Authors: Duchesne Camille , Randuineau Gwénaëlle , Le Normand Laurence , Romé Véronique , Laraqui Samia , Arnaud Alexis Pierre , Boudry Gaëlle 
Published: December 2024

AbstractBackgroundIntestinal organoids are promising tools in the context of animal experiment reduction but a thorough characterization of the impact of the origin of intestinal stem cells (ISC) on organoid phenotype is needed to routinely use this cellular model. Our objective was to evaluate the effect of ISC donor age on the growth, morphology and cellular composition of intestinal organoids derived from pig.MethodsOrganoids were derived from jejunal and colonic ISC obtained from 1‐, 7‐, 28‐, 36‐ and 180‐day‐old pigs and passaged three times.ResultsWe first confirmed by qPCR that the expression of 18% of the >80 studied genes related to various intestinal functions differed between jejunal and colonic organoids after two passages (p < 0.05). Growth and morphology of organoids depended on intestinal location (greater number and larger organoids derived from colonic than jejunal ISC, p < 0.05) but also pig age. Indeed, when ISC were derived from young piglets, the ratio of organoids to spheroids was greater (p < 0.05), spheroids were larger during the primary culture but smaller after two passages (p < 0.05) and organoids were smaller after one passage (p > 0.05) compared to ISC from older pigs. Finally, no difference in cellular composition, evaluated by immunostaining of markers of the major intestinal cell types (absorptive, enteroendocrine and goblet cells) was observed between organoids originating from 7‐ or 180‐day‐old pigs, but differences between intestinal site origins were noticed.ConclusionIn conclusion, while the age of the tissue donor affected organoid growth and morphology, it did not influence the phenotype.
Association of Therapies for Axial Spondyloarthritis on the Risk of Hip and Spine Fractures
Authors: Driscoll Devin , George Navya , Peloquin Christine , Jafarzadeh S. Reza , Liew Jean W. , Dubreuil Maureen 
Published: December 2024

ObjectivePeople with axial spondyloarthritis (axSpA) have increased fracture risk relative to the general population, possibly related to those with chronic inflammation. We assessed the impact of treatment with receiving tumor necrosis factor inhibitors (TNFis) and nonbiologic conventional synthetic disease‐modifying antirheumatic drugs (csDMARDs) on hip and spine fractures in patients with axSpA, relative to receiving nonsteroidal anti‐inflammatory drugs (NSAIDs).MethodsWe conducted a nested case–control study using 2006 to 2021 data from the Merative MarketScan Database. We included adults 18 to 65 years old with at least one inpatient or at least two outpatient axSpA International Classification of Diseases, Ninth Revision (ICD‐9), or International Classification of Diseases, Tenth Revision (ICD‐10), diagnosis codes separated by at least seven days. The primary outcome was hip and/or spine fracture, defined by ICD‐9 or ICD‐10 diagnosis or procedure codes. For each patient with fracture (cases), we selected up to 10 controls without fracture. We evaluated medication exposure (TNFis, csDMARDs, NSAIDs [referent], or none) hierarchically using pharmacy claims and procedure codes. We assessed the relation of medication exposure with hip and spine fracture risk using unconditional logistic regression with confounder adjustment.ResultsOur main analysis included 13,519 individuals with axSpA, comprising 1,229 patients with fracture and 12,290 controls. Individuals receiving TNFis had 29% lower odds of fracture compared to those receiving NSAIDs (odds ratio [OR] 0.71, 95% confidence interval [CI] 0.59–0.85), accounting for age, sex, and diagnosis year. Results were similar in the fully adjusted model (OR 0.75, 95% CI 0.62–0.91) and when stratified by sex.ConclusionUsing a large US insurance claims database, we found evidence for a protective effect of receiving TNFis on fracture risk in patients with axSpA underscoring a potential impact of TNFis in diminishing comorbidities linked with axSpA.
Disentangling the perceptual underpinnings of autism: Evidence from a face aftereffects experiment
Authors: Hennig Julius , Doose Arne , Breier Clara Marie , Soutschek Alexander , Beyer Nicole , Schweinberger Stefan , Kamp‐Becker Ingeborg , Poustka Luise , Albertowski Katja , Roessner Veit , Ehrlich Stefan 
Published: December 2024

AbstractExisting literature has documented diminished norm‐based adaptation (aftereffects) across several perceptual domains in autism. However, the exact underlying mechanisms, such as sensory dominance possibly caused by imprecise priors and/or increased sensory precision, remain elusive. The “Bayesian brain” framework offers refined methods to investigate these mechanisms. This study utilized both model‐free (frequentist statistics) and model‐based (hierarchical Drift Diffusion Modeling) analytical approaches to compare gender face aftereffects in male adolescents with autism (n = 29) to neurotypical controls (n = 39) using a behavioral choice experiment. Contrary to our initial hypotheses, our analyses did not find support for imprecise priors or increased sensory precision within the autistic group. Instead, we observed generally decreased drift rates towards male but not female stimuli in the autistic group. Thus, our findings suggest a lack of own‐gender bias in face processing among the autistic participants. These findings align with more recent behavioral and neurophysiological research observing intact priors in individuals with autism, suggesting that other mechanisms may better explain the perceptual challenges in autism. Our study contributes to the ongoing discourse on perceptual processing in autism, emphasizing the necessity for more nuanced analytical approaches in order to unravel the complexity of this condition.
Business Models for Strong Circularity—The Role of Informative Policy Instruments Promoting Repair
Authors: Bergmann Marcus , Ntsondé Joël , Beulque Rémi , Micheaux Helen 
Published: December 2024

ABSTRACTTo promote the circular economy and change unsustainable business practices, policymakers are increasingly implementing novel policy instruments. Fostering repair is increasingly seen as a concrete strategy towards strong circularity, involving disruptive business model (BM) transformations.This study explores the impact of the recently implemented mandatory repairability index in France on BMs, utilizing a case study methodology with an embedded design.Our findings reveal 19 emerging company practices that affect all main BM dimensions. We identify three ways retailers and manufacturers adopt the index to shift towards repair‐oriented BMs.While the literature on the interplay between public policies and circular BMs has mainly focused on command‐and‐control policies for circular economy strategies other than repair, we show how an informative policy instrument transcends its role as mere consumer information. Instead, it emerges as a versatile management tool, facilitating the adoption or up scaling of diverse repair activities. However, informative instruments do not ensure widespread diffusion of repair‐based BMs. Instead, we emphasize the imperative of promoting repair through a comprehensive and evolving policy mix.
Assessment and Prediction of Salivary Gland Function After Head and Neck Radiotherapy: A Systematic Review
Authors: Le Guevelou J. , Palard‐Novello X. , Kammerer E. , Baty M. , Perazzi M. , Larnaudie A. , De Crevoisier R. , Castelli J. 
Published: December 2024

ABSTRACTBackgroundModern imaging techniques with magnetic resonance imaging (MRI) or positron emission tomography/computed tomography (PET/CT) have recently been developed to assess radiation‐induced damage to salivary structures. The primary aim of this review was to summarize evidence on the imaging modalities used for the assessment and prediction of xerostomia after head and neck radiotherapy (RT).MethodsA systematic review of the literature was performed using successively the MeSH terms “PET,” “MRI,” “scintigraphy,” “xerostomia,” and “radiotherapy.”ResultsSalivary excretion flow following head and neck RT is correlated with the dose delivered to both parotid and submandibular glands. Salivary gland standardized uptake value extracted from PET/CT following RT has been shown to be correlated with SEF. Models including early SUV decline or ADC increase during RT and clinical parameters can help predict the loss of salivary function after RT.ConclusionsModern imaging parameters appear to be correlated with salivary gland scintigraphy parameters. Models including functional parameters extracted from either PET/CT or MRI unveil new possibilities for adaptive treatment in a selected population of patients.
Roles of Copper Transport Systems Members in Breast Cancer
Authors: Chen Yichang , Li Chen , Li Mengxin , Han Bing 
Published: December 2024

ABSTRACTBackgroundThe occurrence and progression of breast cancer are closely linked to copper ion homeostasis. Both copper deficiency and excess can inhibit breast cancer growth, while copper transport systems may contribute to its progression by regulating copper ion transport and the activity of associated proteins. However, a comprehensive review of the roles and applications of copper transport systems in breast cancer remains limited. In this study, we summarize the workflow of copper transport systems and the dual role of copper in cancer, highlighting the contributions of specific members of the copper transport system to breast cancer.MethodsA comprehensive search of the PubMed database was conducted to identify articles published over the past 30 years that focus on the relationship between copper transport system members and breast cancer. The findings were synthesized to elucidate the roles and mechanisms of these transporters in the onset and progression of breast cancer.ResultsWe identified 13 members of the copper transport system associated with the occurrence, progression, and mortality of breast cancer, including SLC31A1, DMT1, ATP7A, ATP7B, MTs, GSH, ATOX1, CCS, COX17, SCO1, SCO2, and COX11. Our findings revealed that, apart from STEAP, the remaining 12 members were overexpressed in breast cancer. These members influence the onset, progression, and cell death of breast cancer by modulating biological pathways such as intracellular copper ion levels and ROS. Notably, we observed for the first time that depletion of the copper storage protein GSH leads to increased copper ion accumulation, resulting in cuproptosis in breast cancer cells.ConclusionBy integrating the members of the copper transport system in breast cancer, we offer novel insights for the treatment of breast cancer and copper‐related therapies.
In Silico Analysis of Curcumin's Targeted Cancer Therapy: Folate Receptor Pathways and Molecular Interaction Insights
Authors: Rajimon K. J. , Almeer Rafa , Thangaiyan Pooventhiran , Khairbek Ali , Thomas Renjith 
Published: December 2024

ABSTRACTThis study explores the therapeutic potential of curcumin (CUR) in cancer therapy, specifically examining its targeted transport through folate receptors and its interaction with certain proteins in breast cancer cell lines. We employed molecular docking technique to assess the binding affinities of CUR with proteins 1H1Q, 1UOM, 4JDD, 5U2D and MCF10A normal breast epithelial cell line protein 5UGB. Out of these, the CUR–1H1Q complex exhibited the greatest binding affinity. To assess the stability of this complex in a biological setting, we conducted molecular dynamics simulations of the 1H1Q–CUR complex for a duration of 100 ns. The simulations demonstrated an extremely stable Cα‐backbone, exhibiting a consistently low root mean square deviation. The radius of gyration measurements suggested a condensed structure with specific areas of flexibility. The simulation revealed a consistent hydrogen bond between CUR and 1H1Q, indicating a robust and long‐lasting interaction between the two molecules. The results indicate that the cytotoxicity of curcumin on MCF7 cancer cell lines is mainly affected by its interactions with several proteins found in these cancer cells. Among the four proteins tested, 1H1Q has the greatest influence. The high affinity of these proteins for curcumin, which results in the creation of stable complexes, seems to trigger cell death. Curcumin's biocompatibility and toxicological effects were investigated in both normal and cancerous cell lines. The study revealed enhanced biocompatibility and potential toxicity in cancerous cell lines, while demonstrating reduced toxicity in normal cell lines.
Synthesis of 1,2,3‐Triazole‐Methyl‐Menadione Derivatives: Evaluation of Electrochemical and Antiparasitic Properties against two Blood‐Dwelling Parasites
Authors: Dupouy Baptiste , Karpstein Tanja , Häberli Cécile , Cal Monica , Rottmann Matthias , Mäser Pascal , Keiser Jennifer , Elhabiri Mourad , Davioud‐Charvet Elisabeth 
Published: December 2024

AbstractThis study explores the synthesis and evaluation of novel 1,2,3‐triazole‐methyl‐1,4‐naphthoquinone hybrids, focusing on their electrochemical properties and antiparasitic efficacies against two human blood‐dwelling parasites Plasmodium falciparum and Schistosoma mansoni. Using copper‐catalyzed azide‐alkyne cycloaddition (CuAAC), a well‐established tool in click chemistry, two synthetic routes were assessed to develop α‐ and β‐[triazole‐methyl]‐menadione derivatives. By optimizing the CuAAC reaction conditions, yields were significantly improved, reaching up to 94 % for key intermediates and resulting in the formation of a library of approximately 30 compounds. Biological evaluation of the compounds in antiparasitic drug assays demonstrated notable antischistosomal potencies, while no significant activity was observed for the same series against P. falciparum parasites. Electrochemical and ‘benzylic’ oxidation studies confirmed that the active ‘benzoyl’ metabolite responsible for the antiplasmodial activity of plasmodione cannot be generated. These findings highlight the potential of triazole‐linked menadione hybrids as promising early candidates for antischistosomal drug development, and provides insights into structure‐activity relationships crucial for future therapeutic strategies.
Association mapping of drought stress response for yield and quality traits in barley
Authors: Sapkota Suraj , Mndolwa Eninka , Hu Gongshe , Fiedler Jason , Nandety Raja Sekhar , Carlson Craig H. , Klos Kathy Esvelt 
Published: December 2024

AbstractBarley (Hordeum vulgare L.) is a major cereal crop grown worldwide for human consumption, malting, and animal feed. Drought is one of the major abiotic stresses that reduce grain yield and quality in barley. This study was conducted to evaluate a set of 250 barley lines grown under irrigated, water‐stressed, and rainfed conditions and to identify genomic regions associated with 10 traits related to grain yield and quality across eight independent field environments. Variability was observed among barley lines for tolerance to water‐stressed conditions in all tested environments. Genotype and environment both contributed to the phenotypic variation of the barley lines. Population structure analysis identified two subpopulations using 20,700 single nucleotide polymorphism (SNP) markers. Genome‐wide association mapping detected 74 significant SNPs (p ≤ 6.5 × 10−6), representing 14 quantitative trait loci (QTLs), on all barley chromosomes, except 3H. The QTL, QBG.ARS.7H, associated with beta‐glucan (BG), was consistently detected across environments and explained 13.93% of phenotypic variation. Carriers of the minor allele for the BG‐associated SNP, JHI‐Hv50k‐2016‐488035, exhibited up to 14.65% higher BG content, on average, compared with carriers of the common allele. This study advances our understanding of the genetics of barley response to water‐stress conditions and suggests molecular markers for QTL, which may be used in barley improvement.
Physiological changes and molecular regulation in sweetpotato responses to low‐temperature stress
Authors: Mu Fangfang , Zheng Hao , Zhao Qiaorui , Li Zongyun 
Published: December 2024

AbstractSweetpotato (Ipomoea batatas [L.] Lam) is highly adaptable to different soils and climates, but it is more sensitive to cold due to its tropical origin. Low‐temperature stress is a key factor affecting storage and has a significant impact on sweetpotato quality. During sweetpotato storage, prolonged exposure to low temperatures causes chilling damage to the root system, altering its physiological functions. This is manifested by wilting of the tuberous roots, nutrient loss, and reduced antioxidant enzyme activity. At the same time, it leads to a significant downregulation of genes associated with cold signaling pathways. Understanding the physiological and molecular mechanisms of sweetpotato's response to low‐temperature stress, which is crucial for improving its quality during storage. In addition, methods such as high‐voltage alternating electric field, controlled atmosphere, hot water treatment, and hot air treatment can better preserve the nutrients of sweetpotato and maintain their high commercial quality during low‐temperature storage. This article reviews and summarizes key studies on the nutrient and physiological changes, as well as the molecular regulatory mechanisms of sweetpotato during low‐temperature storage, and identifies unresolved questions in this field. It provides insights for further research on low‐temperature stress in sweetpotato.
Creation and identification of diploid and tetraploid interspecific hybrids between Oryza sativa and Oryza barthii
Authors: Song Zhaojian , Du Chaoqun , Lv Pincang , Qiao Zhenhua , Liu Keyi , Zhang Xianhua , Wang Wei , He Yuchi , Cai Detian 
Published: December 2024

AbstractWild rice species are invaluable resources for genetic improvement of cultivated rice. “Breeding super rice using double advantages of wide cross and polyploidization” is a novel pathway in rice breeding. To exploit the favorable genes of wild rice fully, a technical system composed of hormone treatment, repeated pollination, hybrid embryo rescue, and chromosome doubling was established for efficient creation of distant hybrids and allopolyploids. Using the technical system, the diploid and tetraploid interspecific hybrids between Oryza sativa L. and Oryza barthii A. Chev. were successfully established. Morphological traits such as lemma tip color, stigma color and exsertion, awn color and length of interspecific hybrids exhibited the characters of their wild rice parents. Some traits such as plant type and grain length were intermediate their parents. Overall, the fertility of hybrids was very low. The results of simple sequence repeat molecular marker detection indicated that the interspecific hybrids possessed the bands of both parents. After chromosome doubling, the grain size, and the seed‐set rates of the tetraploid interspecific hybrids increased significantly, but the tiller number per plant, panicle length, and the total grain number per panicle decreased significantly. The results showed the efficiency and importance of the technical system in establishment of distant hybrids and allopolyploids. The study provides novel germplasm resources for rice breeding and theoretical research.
Predictors of Outcome in Cognitive‐Behavioral Therapy for Avoidant/Restrictive Food Intake Disorder
Authors: Palmer Lilian P. , Kambanis P. Evelyna , Stern Casey M. , Rossman Setareh M. , Mancuso Christopher J. , Andrea Alexandrea M. , Burton‐Murray Helen , Becker Kendra R. , Breithaupt Lauren , Freid Cathryn , Asanza Elisa , Lawson Elizabeth A. , Eddy Kamryn T. , Thomas Jennifer J. 
Published: December 2024

ABSTRACTObjectiveCognitive‐behavioral therapy for avoidant/restrictive food intake disorder (ARFID; CBT‐AR) shows promise in improving clinical outcomes in children/adolescents and adults. We aimed to identify predictors of outcomes in CBT‐AR. We hypothesized that younger age, non‐underweight status, and presence of the fear of aversive consequences profile of ARFID would predict greater likelihood of remission post–treatment, and that presence of the lack of interest in eating/food and sensory sensitivity profiles would predict greater likelihood of persistence post‐treatment. We included sex as an exploratory predictor.MethodIndividuals (N = 94, ages 10–55 years) were offered 20–30 outpatient sessions of CBT‐AR. We collected clinical and demographic data at pre‐treatment, and remission status at post‐treatment.ResultsConsistent with our hypothesis, presence (versus absence) of the fear profile predicted an almost three‐fold increased likelihood of remission. Presence of the sensory profile, lack of interest profile, age, weight status, and sex were not predictors of ARFID outcome.DiscussionThe fear of aversive consequences profile of ARFID may be more amenable to treatment with CBT‐AR. This is the first study to identify predictors of treatment outcome following CBT‐AR. Randomized controlled trials are needed to confirm these findings and examine moderators.
Empagliflozin to prevent worsening of left ventricular volumes and systolic function after myocardial infarction (EMPRESS‐MI)
Authors: Carberry Jaclyn , Petrie Mark C. , Lee Matthew M.Y. , Stanley Bethany , Brooksbank Katriona J.M. , Campbell Ross T. , Good Richard , Jhund Pardeep S. , Kellman Peter , Lang Ninian N. , Lindsay M. Mitchell , Mangion Kenneth , Gardner Roy S. , Mark Patrick B. , Meyer Barbara , O'Donnell Joanne , Orchard Vanessa , Shaukat Aadil , Watkins Stuart , McConnachie Alex , McMurray John J.V. , Welsh Paul , Sattar Naveed , Berry Colin , Docherty Kieran F. 
Published: December 2024

AbstractAimsPatients with a reduced left ventricular ejection fraction (LVEF) following an acute myocardial infarction (MI) are considered to be at risk of progressive adverse cardiac remodelling which can lead to the development of heart failure and death. The early addition of a sodium–glucose cotransporter 2 (SGLT2) inhibitor to standard treatment may delay or prevent progressive adverse remodelling in these patients.Methods and resultsWe performed a randomized, double‐blind, placebo‐controlled, multicentre trial using cardiovascular magnetic resonance imaging (MRI), in patients with left ventricular systolic dysfunction following MI. Eligible patients were those ≥12 h and ≤14 days following acute MI, with an LVEF <45% by MRI. Patients were randomized to empagliflozin 10 mg once a day or matching placebo. The primary outcome was the change in left ventricular end‐systolic volume indexed to body surface area (LVESVI) from baseline to 24 weeks. Secondary outcomes included measures of left ventricular and atrial volumes, left ventricular mass, LVEF, and high‐sensitivity troponin I (hs‐TnI) and N‐terminal prohormone of B‐type natriuretic peptide (NT‐proBNP) concentrations. From October 2022 to January 2024, 105 eligible patients were randomized. The mean age was 63 ± 11 years and 90 (87%) were male. The mean LVEF was 34.8 ± 6.0%. In the placebo group, LVESVI decreased by 7.8 ± 16.3 ml/m2, left ventricular end‐diastolic volume index (LVEDVI) did not change (−0.3 ± 18.7 ml/m2) and LVEF increased by 8.5 ± 7.4% at 24 weeks from baseline. Empagliflozin did not affect the change in LVESVI from baseline to 24 weeks (between‐group difference = 0.3 ml/m2, 95% confidence interval −5.2 to 5.8; p = 0.92). Compared with placebo, empagliflozin also had no effect on LVEDVI, LVEF, left atrial volume index, left ventricular mass index, NT‐proBNP, or hs‐TnI, but did increase haematocrit and reduced uric acid and weight.ConclusionsIn patients with left ventricular systolic dysfunction after an acute MI receiving contemporary standard of care, treatment with empagliflozin had no effect on cardiac volumes or LVEF compared with placebo. Progressive adverse cardiac remodelling did not occur in the majority of patients.