bluuebunny/crossref_metadata_2025_split huggingface dataset:
The experience of Hamad General Hospital collaborative anticoagulation clinic in Qatar during the COVID-19 pandemic
Authors: Abdallah Ibtihal , Eltahir Asma , Fernyhough Liam , El-Bardissy Ahmed , Ahmed Rana , Abdulgelil Mohamed , Elgaily Dina , Mohammed AbdulMoqeeth , Jassim Ameena , Barakat Loluwa , Al-Ansari Mazin , Javed Mehak , Alkhawaja Raja , Elzouki Abdel-nasser 
Published: August 2020

Abstract
Hamad General Hospital Anticoagulation Clinic is one of the largest collaborative-practice clinics of its type in Qatar. The patients being followed at this clinic are typically complex and vulnerable. During the coronavirus disease 2019 pandemic, measures were implemented at the clinic to minimize the exposure of patients and healthcare providers to the acute respiratory syndrome coronavirus-2 and to promote social distancing. These measures included extending INR-recall period, switching to direct oral anticoagulant drugs whenever feasible, home visits to elderly and immunocompromised patients for INR testing, establishing an anticoagulation hotline, and relocation of warfarin dispensing from the main pharmacy to the anticoagulation clinic. In addition, the clinic shifted its multidisciplinary team meetings onto an online platform using Microsoft Teams. Telehealth consultations were extensively utilized to closely follow up with the patients and ensure that anticoagulation efficacy and safety remained optimal. The aim of this paper is to share our experience and describe the measures adopted by the clinic as part of the Hamad Medical Corporation response to the emerging situation.
Discovery of novel SSR markers from transcriptome data of Astronium fraxinifolium Schott, a threatened tree species in Brazil
Authors: Cornacini Maiara , Manoel Ricardo Oliveira , Alcantara Marcelo , Moraes Mário , Silva Edvaldo , Neto Leonel Pereira , Sebbenn Alexandre , Rossini Bruno , Marino Celso 
Published: August 2020

Abstract
Background

Astronium fraxinifolium is an endangered tree species from Brazil. Due to its high importance for environmental reforestation, as well as for the use of its wood, it is necessary to implement management programs for conservation of this species. Simple sequence repeats (SSR) or microsatellite markers have been widely used in population genetic studies across diverse organisms. In this study, we reported for the first time SSR markers for A. fraxinifolium as well as its frequency and distribution from transcriptome data.
Results

More than 125 thousand RNA-seq sequences derived microsatellites, with predominant distribution of trinucleotides repeats. From the initial screening, we selected 20 microsatellite loci, validated and evaluated genetic indexes in two natural populations. All loci were polymorphic, ranging from four to eleven alleles per locus. The observed and expected heterozygosities ranged from 0 to 1.0 and from 0.533 to 1.0, respectively. Genetic differentiation between populations (= 0.363) showed higher diversity within than among populations.
Conclusions

The developed SSR loci from RNA-seq consists in a base for future studies of genetic diversity and population structure, mating system and gene flow in A. fraxinifolium populations as well as related species, aiming the conservation and management of the species.
New insights towards disinfecting viruses – short notes
Authors: Ghernaout Djamel , Elboughdiri Noureddine , Al Arni Saleh 
Published: August 2020

Abstract
Water treatment specialists need more and more to understand how viruses behave in potable water pipes and wastewater setups. This work discusses the late advances in dealing with viruses present in water treatment processes. Activated carbon adsorption (ACA) remains one of the most efficient and credible physicochemical methods. Nanoparticles have been utilized to turn activated carbon into a more efficient sorbent. Membrane filtration could lead to total elimination of viruses and ensure the safety of drinking water plants. As a feasible utilization for disinfecting potable water, solar disinfection (SODIS) remains a green and cost-efficient technology with its optical and thermal pathways and deserves more interest in its large and industrial implementation. Identically, solar distillation remains a viable solution for disinfecting and treating water. The water treatment techniques that are currently utilized for surface water treatment are appropriate for eliminating viruses like influenza A viruses, as proved by the literature. More strict precautions have to be taken to secure viruses' total elimination from water and wastewater as for influenza A and H5N1 in terms of advanced oxidation processes, ACA, and membrane processes application. Before reaching surface water, pathogens have to be removed efficiently from hospital and municipal wastewaters.
Obesity and Insulin Resistance: Associations with Chronic Inflammation, Genetic and Epigenetic Factors
Authors: Gasmi Amin , Noor Sadaf , Menzel Alain , Doşa Alexandru , Pivina Lyudmila , Bjørklund Geir 
Published: August 2020

Background:
Obesity is known to be a multifactorial disease. In its pathogenesis,
different factors such as chronic inflammation, oxidative stress, insulin resistance, genetic factors,
environmental effects, vegetative disturbance, and unbalanced nutrition play a significant
role.

Methodology:
This study describes the association of obesity and insulin resistance with
chronic inflammation, genetic, and epigenetic factors. Previous literature has been reviewed
to explain the relation of obesity with those factors involved in chronic low-grade inflammation
and insulin.

Results:
Obesity is associated with a decrease in ghrelin secretion, elevated plasma leptin levels,
oxidative stress, increased macrophage phagocytic activity, and the induction of proinflammatory
synthesis of cytokines and interferon-gamma. Obesity is linked to decreased
levels of cytochrome P450 (CYP) enzymes and impaired detoxification processes. Deficiency
of vitamins and minerals can also play a significant role in the development of oxidative stress
and chronic inflammation in obesity. There is evidence of associations between a genetic predisposition
to obesity in children with elevated levels of certain miRNAs.

Conclusion:
The purpose of the present review is an analysis of the multiple factors associated
with obesity.
Effect of Capsinoids Supplementation on Fat Oxidation and Muscle Glycogen Restoration During Post-exercise Recovery in Humans
Authors: Liao Su-Fen , Korivi Mallikarjuna , Tsao Jung-Piao , Huang Chun-Ching , Chang Chia-Chen , Cheng I-Shiung 
Published: August 2020

Background:
Capsinoids (CSN), the novel non-pungent capsaicin analogs have been reported to promote
metabolic health and exercise tolerance. However, the effect of CSN on fat oxidation and changes in skeletal
muscle glycogen levels during post-exercise recovery has not been investigated in humans.

Purpose:
We examined the effect of CSN supplementation on energy reliance, glycogen resynthesis and molecular
proteins in the skeletal muscle of young adults during post-exercise recovery.

Methods:
In this crossover-designed study, nine healthy adult male volunteers (aged 21.4±0.2 years, BMI
21.9±1.3 kg/m2) completed a 60-min cycling exercise at 70% VO2max. Participants consumed either CSN (12 mg,
single dosage) or placebo capsules with a high-carbohydrate meal (2 g carb/kg bodyweight) immediately after
exercise. Biopsied muscle samples (vastus lateralis), blood, and gaseous samples were obtained during 3h postexercise
recovery period.

Results:
We found that oral CSN supplementation right after exercise significantly altered the energy reliance on
fat oxidation during recovery. This was evidenced by lower respiratory exchange ratio (RER) and higher fat oxidation
rate in CSN trial. Despite this, acute CSN dosage does not contribute in enhancing the glycogen replenishment
in skeletal muscle during 3h recovery. We identified no significant differences in postprandial glucose
and insulin area under the curve in both trials. Western blot data showed an increased muscle GLUT4 expression,
but no significant response of p-Akt/Akt ratio with CSN during post-exercise recovery.

Conclusion:
Our findings conclude that acute CSN intake could change energy reliance on fat oxidation but is
unable to enhance muscle glycogen resynthesis during post-exercise recovery. Thus, ergogenic properties of CSN
in relevance to muscle glycogen restoration following exercise needs to be further investigated in young adults.
On the Cytotoxicity of Chiral Ruthenium Complexes Containing Sulfur Amino Acids against Breast Tumor Cells (MDA-231 and MCF-7)
Authors: Leite Celisnolia M. , de Araujo-Neto João Honorato , Corrêa Rodrigo S. , Colina-Vegas Legna , Martínez-Otero Diego , Martins Paulo R. , Silva Cristiane G. , Batista Alzir A. 
Published: August 2020

Background:
Breast cancer is one of the most common types among women. Its incidence progressively
increases with age, especially after age 50. Platinum compounds are not efficient in the treatment of breast cancer,
highlighting the use of other metals for the development of new chemotherapeutic agents.

Objective:
This paper aims to obtain three new ruthenium compounds that incorporate sulfur amino acids in their
structures and to investigate their cytotoxic activity in breast tumor cell lines.

Methods:
Complexes with general formula [Ru(AA)(dppb)(bipy)] (complexes 1 and 2) or [Ru(AA)(dppb)
(bipy)]PF6 (complex 3), where AA = L-cysteinate (1), D-penicillaminate (2), and L-deoxyalliinate (3), dppb =
1,4-bis(diphenylphosphino)butane and 2,2´-bipyridine, were obtained from the cis-[RuCl2(dppb)(bipy)] precursor.
The cytotoxicity of the complexes on MDA-MB-231 (triple negative human breast cancer); MCF-7 (double
positive human breast cancer) and V79 (hamster lung fibroblast) was performed by the MTT (4,5-
dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide) method. The control agent was the cisplatin, which is a
commercially available drug for cancer treatment.

Results:
In complexes (1) and (2), the ligands are coordinated to the metal center by nitrogen and sulfur atoms, while in
complex (3) coordination is through the oxygen and nitrogen atoms. These suggestions are based on the infrared and
31P1H NMR data. For complexes (1) and (2), their X-ray structures were determined confirming this suggestion. The
three complexes are stable in a mixture of DMSO (80 %) and biological medium (20 %) for at least 48 h and presented
cytotoxicity against the MDA-MB-231 and MCF-7 tumor cells with reasonable selectivity indexes.

Conclusion:
Our work demonstrated that ruthenium complexes containing sulfur amino acids, bipyridines and
bisphosphines showed cytotoxicity against the MDA-MB-231 and MCF-7 cancer cell lines, in vitro, and that they interact
weakly with the DNA (Deoxyribonucleic Acid) and the HSA (Human Serum Albumin) biomolecules.
Assessment of Male Students’ Basic Badminton Technique Development at High Schools
Authors: Chau Vinh Huy 
Published: August 2020

Aim:
Assessment of male students’ basic badminton technique development at high schools

Objective:
Physical education is an integral part of high school curriculums, contributing to students’ comprehensive development, and badminton is a sport of special interest at this level.

Methods:
However, due to different starting points, academic pressure and lack of frequent practice, students’ badminton training and performance are affected more or less. From this situation, we recognize the need to assess student’s basic techniques in high school badminton teams.

Results:
The study results demonstrate that 3 out of 7 tests to assess male students’ techniques in high school badminton teams show obvious progress and statistical significance.

Conclusion:

In particular, the moving and making straight drop shots has the highest growth rate with W = 13.5%, tcalculated=3.53.
The Technical Feasibility of Integrating Primary Eye Care Into Primary Health Care Systems in Nigeria: Protocol for a Mixed Methods Cross-Sectional Study
Authors: Aghaji Ada , Burchett Helen , Hameed Shaffa , Webster Jayne , Gilbert Clare 
Published: August 2020

BackgroundApproximately 90% of the 253 million blind or visually impaired people worldwide live in low- and middle-income countries. Lack of access to eye care is why most people remain or become blind. The World Health Organization Regional Office for Africa (WHO-AFRO) recently launched a primary eye care (PEC) package for sub-Saharan Africa—the WHO-AFRO PEC package—for integration into the health system at the primary health care (PHC) level. This has the potential to increase access to eye care, but feasibility studies are needed to determine the extent to which the health system has the capacity to deliver the package in PHC facilities.ObjectiveOur objective is to assess the technical feasibility of integrating the WHO-AFRO PEC package in PHC facilities in Nigeria.MethodsThis study has several components, which include (1) a literature review of PEC in sub-Saharan Africa, (2) a Delphi exercise to reach consensus among experts regarding the technical complexity of the WHO-AFRO PEC package and the capacities needed to deliver it in PHC facilities, (3) development of PEC technical capacity assessment tools, and (4) data collection, including facility surveys and semistructured interviews with PHC staff and their supervisors and village health workers to determine the capacities available to deliver PEC in PHC facilities. Analysis will identify opportunities and the capacity gaps that need to be addressed to deliver PEC.ResultsConsensus was reached among experts regarding the technical complexity of the WHO-AFRO PEC package and the capacities needed to deliver it as part of PHC. Quantitative tools (ie, structured questionnaires, in-depth interviews, and observation checklists) and topic guides based on agreed-upon technical capacities have been developed and relevant stakeholders have been identified. Surveys in 48 PHC facilities and interviews with health professionals and supervisors have been undertaken. Capacity gaps are being analyzed.ConclusionsThis study will determine the capacity of PHC centers to deliver the WHO-AFRO PEC package as an integral part of the health system in Nigeria, with identification of capacity gaps. Although capacity assessments have to be context specific, the tools and findings will assist policy makers and health planners in Nigeria and similar settings, who are considering implementing the package, in making informed choices.International Registered Report Identifier (IRRID)DERR1-10.2196/17263
Using Wearable Devices to Monitor Physical Activity in Patients Undergoing Aortic Valve Replacement: Protocol for a Prospective Observational Study
Authors: Lorenzoni Giulia , Azzolina Danila , Fraccaro Chiara , Di Liberti Alessandro , D'Onofrio Augusto , Cavalli Chiara , Fabris Tommaso , D'Amico Gianpiero , Cibin Giorgia , Nai Fovino Luca , Ocagli Honoria , Gerosa Gino , Tarantini Giuseppe , Gregori Dario 
Published: August 2020

Background
In last few decades, several tools have been developed to measure physical function objectively; however, their use has not been well established in clinical practice.

Objective
This study aims to describe the preoperative physical function and to assess and compare 6-month postoperative changes in the physical function of patients undergoing treatment for aortic stenosis with either surgical aortic valve replacement (SAVR) or transcatheter aortic valve replacement (TAVR). The study also aims to evaluate the feasibility of wearable devices in assessing physical function in such patients.

Methods
This is a prospective observational study. The enrollment will be conducted 1 month before patients’ SAVR/TAVR. Patients will be provided with the wearable device at baseline (activity tracker device, Garmin vívoactive 3). They will be trained in the use of the device, and they will be requested to wear it on the wrist of their preferred hand until 12 months after SAVR/TAVR. After baseline assessment, they will undergo 4 follow-up assessments at 1, 3, 6, and 12 months after SAVR/TAVR. At baseline and each follow-up, they will undergo a set of standard and validated tests to assess physical function, health-related quality of life, and sleep quality.

Results
The ethics committee of Vicenza in Veneto Region in Italy approved the study (Protocol No. 943; January 4, 2019). As of October 2020, the enrollment of participants is ongoing.

Conclusions
The use of the wearable devices for real-time monitoring of physical activity of patients undergoing aortic valve replacement is a promising opportunity for improving the clinical management and consequently, the health outcomes of such patients.

Trial Registration
Clinicaltrials.gov NCT03843320; https://tinyurl.com/yyareu5y

International Registered Report Identifier (IRRID)
DERR1-10.2196/20072
Representasi Penindasan Ganda dalam Novel Mirah Dari Banda; Perspektif Feminisme Poskolonial
Authors: Ilma Awla Akbar 
Published: August 2020

This study specifically examines the multiple forms of oppression that is found in the novel Mirah dari Banda by postcolonial feminist perspective. The analysis showed that the novel talk about inferior position of women as a result of the dominance of Dutch and Japanese colonial system and patriarchal domination both colonial and indigenous men. With the two forms of oppression Thus, indigenous women suffer and the lowest level in a colonial situation. Associated with the time of publication, the 1980 novel Mirah dari Banda include nostalgia novel of oppression and the strug gle against colonialism. Thus at the same nostalgic attitude can be regarded as a reflection on the possibility of the presence of the double oppression of women today which synonymous with the era of capitalism (imperialism) and still rooted patriarchal ideology.Penelitian ini secara khusus mengkaji bentuk-bentuk penindasan ganda yang terdapat dalam novel Mirah dari Banda berdasarkan perspektif feminisme poskolonial. Hasil analisis menunjukkan bahwa novel mewacanakan kedudukan inferior perempuan akibat dominasi sistem kolonialisme Belanda dan Jepang serta dominasi patriarki baik lelaki kolonial maupun pribumi. Melalui dua bentuk penindasan demikian, perempuan pribumi menderita dan berada di level terendah dalam situasi kolonial. Terkait dengan waktu penerbitannya, yakni tahun 1980 novel Mirah dari Banda termasuk novel nostalgia atas penindasan dan perjuangan melawan kolonialisme. Sikap nostalgia demikian sekaligus dapat dianggap sebagai refleksi atas kemungkinan hadirnya penindasan penindasan ganda terhadap perempuan saat ini yang identik dengan era kapitalisme (imperialisme) dan masih mengakarnya ideologi patriarki.
Efficacy and Safety of Expanded Hemodialysis with the Theranova 400 Dialyzer
Authors: Weiner Daniel E. , Falzon Luke , Skoufos Line , Bernardo Angelito , Beck Werner , Xiao Mengqi , Tran Ha 
Published: August 2020

Background and objectives
Expanded hemodialysis therapy enabled by medium cut-off membranes may promote greater clearance of larger middle molecules that comprise putative uremic solutes than conventional high-flux dialysis. This randomized trial evaluated the efficacy and safety of hemodialysis treatment with a medium cut-off dialyzer.

Design, setting, participants, & measurements
Clinically stable patients on maintenance hemodialysis were randomized to receive dialysis with either a medium cut-off dialyzer (Theranova 400) or a high-flux dialyzer (Elisio-17H) over 24 weeks of treatment. The primary safety end point was the predialysis serum albumin level after 24 weeks of treatment. The primary efficacy end point was the reduction ratio of free λ light chains at 24 weeks of treatment.

Results
Among 172 patients on maintenance hemodialysis, mean age was 59±13 years, 61% were men, 40% were Black, and mean dialysis vintage was 5±4 years. Of the 86 patients randomized to each dialyzer, 65 completed the trial in each group. The reduction ratio for the removal of free λ light chains was significantly higher in the Theranova 400 group compared with the Elisio-17H group after 4 weeks (39% versus 20%) and 24 weeks (33% versus 17%; both P<0.001). Among secondary end points, the Theranova 400 group demonstrated significantly larger reduction ratios at 4 and 24 weeks for complement factor D, free κ light chains, TNFα, and β2-microglobulin (P<0.001 for all), but not for IL-6. Predialysis serum albumin levels were similar between groups after 24 weeks (4 g/dl with the Theranova 400 and 4.1 g/dl with the Elisio-17H), consistent with noninferiority of the Theranova 400 dialyzer in maintaining predialysis serum albumin levels after 24 weeks of treatment.

Conclusions
Hemodialysis therapy with the Theranova 400 dialyzer provides superior removal of larger middle molecules, as exemplified by free λ light chains, compared with a similar size high-flux dialyzer, while maintaining serum albumin level.

Clinical Trial registry name and registration number
A Multi-Center, Prospective, Randomized, Controlled, Open-Label, Parallel Study to Evaluate the Safety and Efficacy of the Theranova 400 Dialyzer in End Stage Renal Disease (ESRD) Patients, NCT03257410.
Implementing a Patient-Reported Outcome Measure for Hemodialysis Patients in Routine Clinical Care
Authors: Evans Jenna M. , Glazer Alysha , Lum Rebecca , Heale Esti , MacKinnon Marnie , Blake Peter G. , Walsh Michael 
Published: August 2020

Background and objectives
The Edmonton Symptom Assessment System Revised: Renal is a patient-reported outcome measure used to assess physical and psychosocial symptom burden in patients treated with maintenance dialysis. Studies of patient-reported outcome measures suggest the need for deeper understanding of how to optimize their implementation and use. This study examines patient and provider perspectives of the implementation process and the influence of the Edmonton Symptom Assessment System Revised: Renal on symptom management, patient-provider communication, and interdisciplinary communication.

Design, setting, participants, & measurements
Eight in-facility hemodialysis programs in Ontario, Canada, assessed patients using the Edmonton Symptom Assessment System Revised: Renal every 4–6 weeks for 1 year. Screening and completion rates were tracked, and pre- and postimplementation surveys and midimplementation interviews were conducted with patients and providers. A chart audit was conducted 12 months postimplementation.

Results
In total, 1459 patients completed the Edmonton Symptom Assessment System Revised: Renal; 58% of eligible patients completed the preimplementation survey (n=718), and 56% of patients who completed the Edmonton Symptom Assessment System Revised: Renal at least once completed the postimplementation survey (n=569). Provider survey response rates were 71% (n=514) and 54% (n=319), respectively. Nine patients/caregivers from three sites and 48 providers from all sites participated in interviews. A total of 1207 charts were audited. Seven of eight sites had mean screening rates over 80%, suggesting that routine use of the Edmonton Symptom Assessment System Revised: Renal in clinical practice is feasible. However, the multiple data sources painted an inconsistent picture of the value and effect of the Edmonton Symptom Assessment System Revised: Renal. The Edmonton Symptom Assessment System Revised: Renal standardized symptom screening processes across providers and sites; improved patient and provider symptom awareness, particularly for psychosocial symptoms; and empowered patients to raise issues with providers. Yet, there was little, if any, statistically significant improvement in the metrics used to assess symptom management, patient-provider communication, and interdisciplinary communication.

Conclusions
The Edmonton Symptom Assessment System Revised: Renal patient-reported outcome measure may be useful to standardize symptom screening, enhance awareness of psychosocial symptoms among patients and providers, and empower patients rather than to reduce symptom burden.