bluuebunny/crossref_metadata_2025_split huggingface dataset:
High-Dose Melatonin and Ethanol Excipient Combined with Therapeutic Hypothermia in a Newborn Piglet Asphyxia Model
Authors: Robertson Nicola J. , Lingam Ingran , Meehan Christopher , Martinello Kathryn A. , Avdic-Belltheus Adnan , Stein Liane , Tachrount Mohamed , Price David , Sokolska Magdalena , Bainbridge Alan , Hristova Mariya , Fleiss Bobbi , Kramer Boris W. , Gressens Pierre , Golay Xavier 
Published: March 2020

AbstractWith the current practice of therapeutic hypothermia for neonatal encephalopathy, disability rates and the severity spectrum of cerebral palsy are reduced. Nevertheless, safe and effective adjunct therapies are needed to optimize outcomes. This study’s objective was to assess if 18 mg/kg melatonin given rapidly over 2 h at 1 h after hypoxia-ischemia with cooling from 1–13 h was safe, achieved therapeutic levels within 3 h and augmented hypothermic neuroprotection. Following hypoxia-ischemia, 20 newborn piglets were randomized to: (i) Cooling 1–13 h (HT; n = 6); (ii) HT+ 2.5% ethanol vehicle (HT+V; n = 7); (iii) HT + Melatonin (HT+M; n = 7). Intensive care was maintained for 48 h; aEEG was acquired throughout, brain MRS acquired at 24 and 48 h and cell death (TUNEL) evaluated at 48 h. There were no differences for insult severity. Core temperature was higher in HT group for first hour after HI. Comparing HT+M to HT, aEEG scores recovered more quickly by 19 h (p < 0.05); comparing HT+V to HT, aEEG recovered from 31 h (p < 0.05). Brain phosphocreatine/inorganic phosphate and NTP/exchangeable phosphate were higher at 48 h in HT+M versus HT (p = 0.036, p = 0.049 respectively). Including both 24 h and 48 h measurements, the rise in Lactate/N-acetyl aspartate was reduced in white (p = 0.030) and grey matter (p = 0.038) after HI. Reduced overall TUNEL positive cells were observed in HT+M (47.1 cells/mm2) compared to HT (123.8 cells/mm2) (p = 0.0003) and HT+V (97.5 cells/mm2) compared to HT (p = 0.012). Localized protection was seen in white matter for HT+M versus HT (p = 0.036) and internal capsule for HT+M compared to HT (p = 0.001) and HT+V versus HT (p = 0.006). Therapeutic melatonin levels (15–30mg/l) were achieved at 2 h and were neuroprotective following HI, but ethanol vehicle was partially protective.
Difference in post-stress recovery of the gut microbiome and its altered metabolism after chronic adolescent stress in rats
Authors: Xu Mengyang , Wang Chen , Krolick Kristen N. , Shi Haifei , Zhu Jiangjiang 
Published: March 2020

AbstractThe human gut microbiome plays a central role in human health, and has been implicated in the development of a number of chronic gastrointestinal and systemic diseases. For example, microorganisms can serve as microbial endocrine mediators and can respond to stimuli and produce neurochemicals, ultimately influencing the brain-gut-microbiome axis of their host, a bidirectional communication system between the central nervous system and the gastrointestinal tract, especially during developmental stages. To begin to explore potential dynamic changes of the gut microbiome, we characterized gut microbiota in adolescent rats that underwent a fixed period of restraint stress, examined whether the gut microbial population and their metabolic functions were changed by stress, and if such changes during adolescence persist or recover in young adulthood. Integrated 16S ribosomal DNA sequencing and liquid chromatography coupled tandem mass spectrometry (LC-MS/MS) based metabolic profiling were utilized to discover any significant differences in gut microbial genus and microbial metabolites immediately at the end of the chronic restraint stress and three weeks after the stress treatment, compared to control rats that did not receive stress treatment. Interestingly, while adolescent chronic stress-induced differences in relative microbial abundance (i.e., microbial species and distribution) disappeared three weeks after the stress treatment ended, the differences in microbial metabolic profiles persisted into adulthood. In addition, a number of significantly altered metabolites and their correlated gut microbes detected in our study facilitated a possible connection between gut microbiota and host stress response, which can be further investigated in the future to study the causal relationship between gut microbial metabolites and their impact on human health.
Auxin perception in Agave is dependent on the species’ Auxin Response Factors
Authors: Cancino-García Víctor J. , Ramírez-Prado Jorge H. , De-la-Peña Clelia 
Published: March 2020

AbstractAuxins are one of the most important and studied phytohormones in nature. Auxin signaling and perception take place in the cytosol, where the auxin is sensed. Then, in the nucleus, the auxin response factors (ARF) promote the expression of early-response genes. It is well known that not all plants respond to the same amount and type of auxins and that the response can be very different even among plants of the same species, as we present here. Here we investigate the behavior of ARF in response to various auxins inAgave angustifoliaHaw.,A.fourcroydesLem. andA.tequilanaWeber var. Azul. By screening the available database ofA.tequilanagenes, we have identified 32ARFgenes with high sequence identity in the conserved domains, grouped into three main clades. A phylogenetic tree was inferred from alignments of the 32 Agave ARF protein sequences and the evolutionary relationship with other species was analyzed.AteqARF4, 15, 21, and 29 were selected as a representative diverse sample coming from each of the different subclades that comprise the two main clades of the inferred phylogenetic reconstruction. TheseARFs showed differential species-specific expression patterns in the presence of indole-3-acetic acid (IAA) and 2,4-dichlorophenoxyacetic acid (2,4-D). Interestingly,A.angustifoliashowed different phenotypes in the presence and absence of auxins. In the absence of auxin,A.angustifoliaproduces roots, while shoots are developed in the presence of IAA. However, in the presence of 2,4-D, the plant meristem converts into callus. According to our results, it is likely thatAteqARF15participates in this outcome.
64-Channel Carbon Fiber Electrode Arrays for Chronic Electrophysiology
Authors: Guitchounts Grigori , Cox David 
Published: March 2020

AbstractA chief goal in neuroscience is to understand how neuronal activity relates to behavior, perception, and cognition. However, monitoring neuronal activity over long periods of time is technically challenging, and limited, in part, by the invasive nature of recording tools. While electrodes allow for recording in freely-behaving animals, they tend to be bulky and stiff, causing damage to the tissue they are implanted in. One solution to this invasiveness problem may be probes that are small enough to fly under the immune system's radar. Carbon fiber (CF) electrodes are thinner and more flexible than typical metal or silicon electrodes, but the arrays described in previous reports had low channel counts and required time-consuming manual assembly. Here we report the design of an expanded-channel-count carbon fiber electrode array (CFEA) as well as a method for fast preparation of the recording sites using acid etching and electroplating with PEDOT-TFB, and demonstrate the ability of the 64-channel CFEA to record from rat visual cortex. We include designs for interfacing the system with micro-drives or flex-PCB cables for recording from multiple brain regions, as well as a facilitated method for coating CFs with the insulator Parylene-C. High-channel-count CFEAs may thus be an alternative to traditional microwire-based electrodes and a practical tool for exploring the neural code.
Annotation and cluster analysis of long noncoding RNA linked to male sex and estrogen in cancers
Authors: Liu Shouping , Lai Weiwei , Shi Ying , Liu Na , Ouyang Lianlian , Zhang Ziying , Chen Ling , Wang Xiang , Qian Banglun , Xiao Desheng , Yan Qin , Cao Ya , Liu Shuang , Tao Yongguang 
Published: March 2020

AbstractThe sex difference in cancer occurrence is a consistent finding in cancer epidemiology. Several solid tumors, including lung cancer, colorectal cancer, hepatic carcinoma, and renal carcinoma, are generally more common in males. Although sexual dimorphism is attributed to hormonal or behavioral differences, evidence for the function of lncRNA is lacking in sex-specific cancers. We show here that LINC00263 is one of the most dysregulated lncRNAs in lung adenocarcinomas and is upregulated in lung adenocarcinoma, colorectal cancer, and renal carcinoma, especially in male patients compared to females. LINC00263 functions as an oncogene by promoting translocation of p65 into the nucleus to activate the NF-κB-signaling pathway through interaction with IKKα in the cytoplasm. The expression of LINC00263 is strongly correlated with ESR1, and it is decreased after treatment with estrogen. Ligand-activated ER could inhibit the function of LINC00263 by inhibiting NF-κB from cytoplasmic translocation into the nucleus. The inhibitory effect of estrogen on LINC00263 indicates its differential expression in male and female patients. Our findings indicate that LINC00263 is linked to male sex and estrogen as an oncogene, and these findings might help in the exploration of the mechanisms of differential gene regulation in sex-specific cancers.
Comprehensive comparisons between frequency‐domain analysis and time‐domain analysis for LLC resonant converter
Authors: Wei Yuqi , Luo Quanming , Mantooth Alan 
Published: March 2020

LLC resonant converter has been widely used in many applications due to its characteristics of high efficiency and high frequency characteristics. Analysis method plays great role in converter design and operation performance. For LLC resonant converter, the most frequently used analysis methods are frequency‐domain analysis (FDA) and time‐domain analysis (TDA). FDA assumes that only fundamental harmonics can deliver energy in the system, while TDA is obtained by solving circuit state equations. In this study, comprehensive and complete comparisons between FDA and TDA in terms of analysis methodology, parameter design, analysis accuracy, conduction loss, other power losses, and system efficiency are implemented. The analysis and comparison results show that under the same system specifications, by adopting TDA, a larger magnetising inductance value can be used when compared with FDA, which will reduce the resonant tank circulating current and increase the system efficiency. The comprehensive analysis can provide guidance for engineers when analysing and designing LLC resonant converter. Finally, a 200 W experimental prototype was built and tested to verify the theoretical analysis.
Structure and kinematics of the Virgo cluster of galaxies
Authors: Kashibadze Olga G. , Karachentsev Igor D. , Karachentseva Valentina E. 
Published: March 2020

Aims. This work considers the Virgo cluster of galaxies, focusing on its structure, kinematics, and morphological landscape. Our principal aim is to estimate the virial mass of the cluster. For this purpose, we present a sample of 1537 galaxies with radial velocities VLG <  2600 km s−1 situated within a region of ΔSGL = 30° and ΔSGB = 20° around M 87. About half of the galaxies have distance estimates.
Methods. We selected 398 galaxies with distances in a (17 ± 5) Mpc range. Based on their 1D and 2D number-density profiles and their radial velocity dispersions, we made an estimate for the virial mass of the Virgo cluster.
Results. We identify the infall of galaxies towards the Virgo cluster core along the Virgo Southern Extension filament. From a 1D profile of the cluster, we obtain the virial mass estimate of (6.3 ± 0.9)×1014 M⊙, which is in tight agreement with its mass estimate via the external infall pattern of galaxies.
Conclusions. We conclude that the Virgo cluster outskirts between the virial radius and the zero-velocity radius do not contain significant amounts of dark matter beyond the virial radius.
Diagnostik und Therapie unklarer Gallengangsstenosen
Authors: Krause Jenny , Ehlken Hanno , Schramm Christoph 
Published: March 2020

AbstractBiliary stenoses represent a differential diagnostic challenge. Diagnostic methods to clarify the underlying dignity are often invasive, and provide high specificity beside insufficient sensitivity. In many cases, an accurate diagnosis is only possible over time, and therefore limits curative treatment options. This article provides an overview of the diagnostic challenges and treatment options for unclear biliary stenosis.
Das Mukoepidermoidkarzinom – Bedeutung der Molekularpathologie
Authors: Nachtsheim Lisa , Arolt Christoph , Dreyer Thomas , Meyer Moritz F. , Brobeil Alexander , Gamerdinger Ulrike , Gattenlöhner Stefan , Grosheva Maria , Wittekindt Claus , Klußmann Jens Peter 
Published: March 2020

ZusammenfassungDas Mukoepidermoidkarzinom ist das häufigste primäre Speicheldrüsenmalignom im Kopf-Hals-Bereich. Das Tumorgrading hat eine erhebliche prognostische Bedeutung. Die 5-Jahres-Überlebensrate ist für Low-grade-Mukoepidermoidkarzinome deutlich höher als für Intermediate-grade- oder High-grade-Mukoepidermoidkarzinome. Für das Mukoepidermoidkarzinom scheint die Translokation von t(11;19)(q21;p13) mit der resultierenden CRTC1-MAML2-Transfusion ebenfalls von prognostischer Relevanz zu sein. Diese Translokation ist in 38–82 % aller Mukoepidermoidkarzinome nachweisbar. In Studien konnte für Patienten mit CRTC1-MAML2-positiven Mukoepidermoidkarzinomen eine deutlich bessere Prognose als für Patienten mit Translokations-negativen Mukoepidermoidkarzinomen nachgewiesen werden. Die t(11;19)(q21;p13) -Translokation wird deutlich häufiger bei Low- und Intermediate-grade-Mukoepidermoidkarzinomen detektiert als bei High-grade-Tumoren derselben Entität. Darüber hinaus wurden Translokations-positive Mukoepidermoidkarzinome häufiger bei jüngeren Patienten, kleineren Tumoren und niedrigeren Tumorstadien diagnostiziert sowie seltener Lymphknoten- und Fernmetastasierung gefunden. Bislang ist die Translokation nicht von therapeutischer Bedeutung. Jedoch sollte aufgrund der prognostischen Relevanz eine Diagnostik regelhaft erfolgen. Bei Grenzfällen kann der fehlende Nachweis der Translokation eine Entscheidungshilfe zu einer Therapieeskalation geben. Um den individuellen prognostischen und therapeutischen Wert des Fusionsgens genauer zu beurteilen, werden weitere Untersuchungen notwendig sein.
Sustained colonoscopy quality improvement using a simple intervention bundle
Authors: Neilson Laura J. , East James E. , Rajasekhar Praveen T. , Bassett Paul , Dunn Simon , Bevan Roisin , Paremal Shyju , Esmaily Shiran , Rees Colin J. 
Published: March 2020

Abstract
Background Unacceptable variation in colonoscopy quality exists. The Quality Improvement in Colonoscopy (QIC) study in 2011 improved quality by introducing an evidence-based “bundle” of measures into routine colonoscopy practice. The QIC bundle included: minimal cecal withdrawal time of ≥ 6 minutes; hyoscine butylbromide use; supine patient position for transverse colon examination; rectal retroflexion. Colonoscopy quality was measured by adenoma detection rate (ADR). The current study measured whether these effects led to a sustained change in practice 3 years following implementation.
Methods This observational study collected data from eight hospital trusts (sites) in the United Kingdom for a 6-month period, 3 years following QIC bundle implementation. Use of the antispasmodic, hyoscine butylbromide, was measured as a marker of bundle uptake. Bundle effectiveness was measured by ADR change. Comparisons were made between data before and immediately after implementation of the bundle.
Results 28 615 colonoscopies by 188 colonoscopists were studied. Hyoscine butylbromide use increased from 15.8 % pre-implementation to 47.4 % in the sustainability phase (P < 0.01) indicating sustained engagement with QIC measures. ADR was higher in the sustainability period compared with pre-intervention, but only reached statistical significance among the poorest-performing colonoscopists.
Conclusions The introduction of a simple, inexpensive, pragmatic intervention significantly changed practice over a sustained period, improving colonoscopy quality as measured by ADR, particularly in poorer performers. QIC demonstrates that an easy-to-implement quality improvement approach can deliver a sustained change in practice for many years post intervention.
Bildgebende Diagnostik des Innenohrs vor Cochleaimplantation
Authors: Mack Martin G. , Suckfüll Markus M. 
Published: March 2020

AbstractIf sufficient speech understanding can no longer be achieved with conventional hearing aids, the indication for a cochlear implant is very often present. Imaging plays an important preoperative role, as it is the only way to decide whether a cochlear implant is possible or not. The preoperative evaluation of the temporal bone for the precise selection of the candidates for cochlear implant is including CT (Computed Tomography) and MRI (Magnetic Resonance Imaging).
Cook–Swartz Doppler Probe Surveillance for Free Flaps—Defining Pros and Cons
Authors: Paprottka Felix J. , Klimas Dalius , Krezdorn Nicco , Schlarb Dominik , Trevatt Alexander E. J. , Hebebrand Detlev 
Published: March 2020

Abstract
Introduction The main postoperative complication of free flaps is perfusion compromise. Urgent intervention is critical to increase the chances of flap survival. Invasive flap perfusion monitoring with direct blood flow feedback through the Cook–Swartz Doppler probe could enable earlier detection of perfusion complications.
Materials and Methods Between 2012 and 2016, 35 patients underwent breast reconstruction or defect coverage after trauma with a deep inferior epigastric perforator, anterolateral thigh, transverse musculocutaneous gracilis, gracilis, or latissimus dorsi flap in our department. All flaps were monitored with a Cook–Swartz probe for 10 days postoperatively. The 20 MHz probe was placed around the arterial–venous anastomosis. A flap monitoring protocol was established for standardized surveillance of postoperative perfusion. In the event of probe signal loss, immediate surgical revision was initiated.
Results Signal loss was detected in 8 of the 35 cases. On return to the operating room, six were found to be true positives (relevant disruption of flap perfusion) and two were false positives (due to Doppler probe displacement). There were also two false negatives, resulting in a slowly progressive partial flap loss. Flap perfusion was restored in three of the six cases (50%) identified by the probe. Following surgical intervention, three of the six cases had persistent problems with perfusion, resulting in two total flap losses and one partial flap necrosis leading to an overall 5.7% total flap loss.
Conclusion Postoperative flap perfusion surveillance is a complex matter. Surgical experience is often helpful but not always reliable. The costs, false-positive, and false-negative rates associated with invasive perfusion monitoring with Cook–Swartz probe make it most appropriate for buried flaps.
Level of Evidence This is an original work.
Human Dirofilariasis Due to D.Repens Presenting as a Chest Wall Swelling : A Rare Case Report
Authors: Permi Harish S. , D'Souza Pretty , Bhagavan K.R. , Raju Mary , Sarda Pooja 
Published: March 2020

AbstractPrimary Dirofilariasis is caused by a Zoonotic filarial nematode. It is transmitted to humans by Culex, Aedes, or Anopheles mosquitoes, which ingest blood-containing microfilaria from affected dogs, cats, or raccoons. Chest wall tumors are uncommon lesions that originate from blood vessels, nerves, bone, cartilage, or fat. We report a case of Human Dirofilariasis due to D. Repens occurring in the chest wall in a 32 year old male. Clinical diagnosis of benign chest wall tumor was considered and it was excised. Histopathological examination confirmed it as Dirofilaria repens. On regular follow up he is doing fine.
PREVALENCE OF AUTISM AUTISTIC FEATURES AND ASSOCIATED RISK FACTORS IN SUBJECTS ATTENDING SPECIAL SCHOOLS IN OUR COMMUNITY
Authors: V. Sharda , K. Subbalakshmi N. , S. Narayana , K. Samal R. 
Published: March 2020

Abstract
Introduction: There is no one single unified theory to explain the etiology of autism. Autistic symptoms occur along a wide spectrum. Thus we studied the autistic symptoms according to gender, mental retardation and presence of epilepsy.
Methods: 500 children attending different special schools were screened for autism employing DSM-IV diagnostic criteria for autistic disorder. Data was analysed employing suitable statistical tests. P value less than 0.05 was considered significant.
Results: Display of autistic features was significantly different among the study subjects (p value less than 0.00001). But more than 90% of the subjects had distorted speech, repetitive speech and tantrum to minor changes symptoms. Autistic males were two times greater than autistic females (50 vs. 24). Proportion of autistic subjects with loneliness and poor interaction was significantly higher in 26-50 intelligence quotients group compared to 51-80 intelligence quotients (p value equal to 0.002, 0.03 respectively). 25.67% autistic subjects were epileptic. In them, prevalence of not identifying strangers, failing to make eye contact, not understanding humor symptoms were significantly higher compared to others (p value equal to 0.018, 0.044, 0.015, respectively). Epilepsy was associated with higher number of autism symptoms (p value equal to 0.023).
Conclusions: Tantrum to minor changes, distorted and repetitive speech are the most common autistic features. Risk of autism is 2 times higher in males compared to females. Aloofness and poor interaction symptoms are higher in autistic subjects with lower intelligence quotients. Epilepsy is associated with higher number of autism symptoms.
Comparative Study of Student-Led Objective Tutorials versus Traditional Tutorials in Undergraduate Pharmacology Teaching in an Indian Medical School
Authors: Adiga Manoor Narasimha Sachidananda , Acharya Swathi , Holla Rajendra 
Published: March 2020

Abstract
Introduction The drawback of conventional tutorials (CTs) stresses the need for a tutorial with more active participation from students. Student-led objective tutorial provides an opportunity for active learning and makes student responsible. Hence, the effectiveness of student-led objective tutorial (SLOT) with CT in teaching pharmacology for II MBBS students was evaluated in this study.
Materials and Methods In this study, 157 students were divided into three groups and further allocated to SLOT and CT subgroup equally. The effectiveness of the tutorial was evaluated objectively by comparing pretest and posttest scores as well as mean scores.
Results Pretest and posttest scores in SLOT were 3.343 ± 1.347 versus 4.8750 ± 1.750 and in CT were 3.2500 ± 1.690 versus 4.5781 ± 1.883, both type of the tutorials have shown the statistically significant improvement in the scores. The mean score in the SLOT group was 13.6711 ± 3.47712 compared with 12.1842 ± 3.50128 in the CT group showing statistically significantly (U = 2171.50, p = 0.008) higher score in SLOT. Feedback regarding the type of tutorial indicating SLOT was better and preferred among the students compared with CT. It is more interesting, helps in active learning of the subject, and encourages student to participate actively in learning process.
Conclusion SLOT and CT both improve the learning pharmacology in II MBBS students; however, SLOT being an innovative method will help in active participation of students and increases the focus of the student on particular topic in teaching.