bluuebunny/crossref_metadata_2025_split huggingface dataset:
Preoperative circulating tumor cells to predict microvascular invasion and dynamical detection indicate the prognosis of hepatocellular carcinoma
Authors: Zhou Jiangmin , Zhang Zhiwei , Zhou Honghao , Leng Chao , Hou Bingwu , Zhou Chenyang , Hu Xinsheng , Wang Jinlin , Chen Xiaoping 
Published: September 2020

Abstract
Background: This study explored the diagnostic power of preoperative circulating tumor cells (CTCs) for the presence of microvascular invasion (MVI) and the relationship between dynamic changes in postoperative CTCs and prognosis. Methods: A total of 137 patients were recruited for the study. Preoperative blood samples of all patients were collected to detect CTC. The time points for blood collection were before the operation, during operation, and at one week, one month, two months, three months, six months, and one year after surgery. The power of predicting the presence of MVI was analyzed by the receiver operating characteristic curve (ROC). According to the survival status, 137 patients were divided into three groups: no recurrence, early recurrence, and non-early recurrence. Results: A threshold CTC value of 5 showed the most significant power to predict the existence of MVI. In a multivariate analysis, the parameters of preoperative CTCs, alpha-fetoprotein (AFP) and tumor diameter were independent predictors of MVI ( P < 0.05). A CTC value greater than or equal to 5 has better predictive value than AFP > 400μg/L or tumor diameter > 5 cm. The number of intraoperative CTCs in the three groups did not increase compared to before surgery ( P > 0.05). The number of CTC in the non-recurrent group and the non-early recurrent group decreased significantly one week after surgery compared with intraoperative values ( P < 0.001), although there was no statistical significance in the group with early recurrence ( P = 0.95). Patients with mean CTC ≥ 5 had significantly worse long-term outcome than those with mean CTC < 5 ( P <0.001).Conclusion: The preoperative CTC counts in peripheral blood of patients with HCC is closely correlated to MVI. Intraoperative manipulation for the lesion by the surgeon does not increase the number of CTC in peripheral blood. Surgical removal of the tumor decreases the number of CTC. The persistence of CTC at a high level (≥ 5) after surgery suggests a risk of early recurrence.
Reactive Attachment Disorder Symptoms and Prosocial Behavior in Middle Childhood: The Role of Secure Base Script Knowledge&nbsp;
Authors: Cuyvers Bien , Vervoort Eleonore , Bosmans Guy 
Published: September 2020

Abstract
Background: Children with attachment disorder show prosocial behavior problems. Children with a reactive attachment disorder show inhibited and emotionally withdrawn behavior. Consequently, these children typically display prosocial behavior problems. However, the underlying mechanism between reactive attachment disorder and prosocial behavior problems is still unclear and findings in literature are mixed. Methods: The current study investigated the role of children’s attachment representations in this association. Attachment representations reflect knowledge about a cognitive script regarding the attachment figure as a source for support (Secure Base Script). We tested whether secure base script knowledge 1) mediates or 2) moderates the link between reactive attachment disorder and prosocial behavior problems in 83 children (6-11 years; 83.1% boys) recruited from special education schools for children with behavioral problems. Children completed a pictorial Secure Base Script Test. Their reactive attachment disorder symptoms were assessed during an interview with the primary caregivers. Primary caregivers and teachers filled out a prosocial behavior questionnaire about the child. Results: Results did not support the mediation hypothesis, but evidence for the moderation hypothesis was found. Secure base script knowledge attenuated the negative association between attachment disorder symptoms and prosocial behavior. Conclusions: These findings contribute to the discussion about the link between attachment representations and attachment disorders.
WRN modulates translation by influencing nuclear mRNA export in HeLa cancer cells
Authors: Iglesias-Pedraz Juan Manuel , Jara Diego Matia Fossatti , Felice Valeria Del Carmen Valle-Riestra , Visalaya Sergio Rafael Cruz , Felix Jose Antonio Ayala , Comai Lucio 
Published: September 2020

Abstract
Background The Werner syndrome protein (WRN) belongs to the RecQ family of helicases and its loss of function results in the premature aging disease Werner syndrome (WS). We previously demonstrated that an early cellular change induced by WRN depletion is a posttranscriptional decrease in the levels of enzymes involved in metabolic pathways that control macromolecular synthesis and protect from oxidative stress. This metabolic shift is tolerated by normal cells but causes mitochondria dysfunction and acute oxidative stress in rapidly growing cancer cells, thereby suppressing their proliferation.Results To identify the mechanism underlying this metabolic shift, we examined global protein synthesis and mRNA nucleocytoplasmic distribution after WRN knockdown. We determined that WRN depletion in HeLa cells attenuates global protein synthesis without affecting the level of key components of the mRNA export machinery. We further observed that WRN depletion affects the nuclear export of mRNAs and demonstrated that WRN interacts with mRNA and the Nuclear RNA Export Factor 1 (NXF1).Conclusions Our findings suggest that WRN influences the export of mRNAs from the nucleus through its interaction with the NXF1 export receptor thereby affecting cellular proteostasis. In summary, we identified a new partner and a novel function of WRN, which is especially important for the proliferation of cancer cells.
The influence of gingival phenotype on the morphology of the maxillary central papilla
Authors: Belák Šimon , Žižka Radovan , Starosta Martin , Zapletalová Jana , Šedý Jiří , Štefanatný Michal 
Published: September 2020

Abstract
Background Preservation of the interdental papilla is an essential part of the functional and esthetic rehabilitation of dental treatment. It has been described that thicker gingival tissues are more resistant to recession. The main objective of this investigation was to analyze whether a thin gingival phenotype represents a potential risk factor affecting interdental papilla fill, height, or width in an esthetic region between maxillary central incisors. The secondary goals were: 1) to analyze parameters describing the papilla – fill, height, width, and effect of papilla base width on the vertical papillary dimension; 2) to determine correlation between different non-invasive measurements of gingival thickness; 3) to compare both sexes.Methods A total of 54 periodontally healthy students (20–30 years old) were included in the study. Gingival thickness was measured using Pirop Ultrasonic Biometer. Gingival phenotype was also assessed by gingival probe transparency. Papilla height and width were measured, and the degree of papilla recession was classified.Results No significant correlation between papilla fill, height, width and gingival probe transparency or gingival thickness was found. Gingival thickness and gingival probe transparency showed a significant relationship (P < 0.001). There was a significant correlation between papilla height and papilla fill (P = 0.028). A papilla which filled the interdental space completely seemed to be shorter. A strong positive association between papilla height and papilla width was found (P <0.0001). The papilla between maxillary central incisors was significantly higher in males (P = 0.01).Conclusion The appearance of the interdental papilla may be influenced by various factors. Within the limitations of this study, the results showed that the thin gingival phenotype alone is no potential risk factor affecting interdental papilla fill, height, or width. There seems to be an effect of papilla base width on its vertical dimension. Gingival probe transparency is a simple reliable method of assessment of gingival thickness with a threshold value of 1-mm gingival thickness between the thick and thin phenotypes.
Dental caries in association with viral load in children living with HIV in Phnom Penh, Cambodia: a cross-sectional study
Authors: Kikuchi Kimiyo , Yasuoka Junko , Tuot Sovannary , Okawa Sumiyo , Yem Sokunthea , Chhuon Pheak , Murayama Makoto , Huot Chantheany , Yi Siyan 
Published: September 2020

Abstract
Background: Oral health status is known to be associated with overall health among people living with HIV. However, it is unclear whether dental caries is associated with viral load among this population. In particular, dental caries among children living with HIV needs to be better understood, as this can affect their overall health and future well-being. This study assessed the association between dental caries and viral load among children living with HIV in Phnom Penh, Cambodia.Methods: This cross-sectional study was conducted at the National Paediatric Hospital as a baseline survey of a randomized controlled trial. The study population included children living with HIV aged 3–15 years and their primary caregivers. We collected data on the children’s oral health status by oral examination and the latest HIV viral load data stored in the patients’ information system at the hospital. We also conducted a questionnaire-based interview of the children and their primary caregivers. Multiple logistic regression analysis was conducted to assess the association between dental caries and viral load. The cut-off point for undetectable viral load was set at <40 copies/mL.Results: Data from 328 children were included in the analysis; 68.3% had an undetectable viral load. The mean number of permanent or deciduous teeth with caries was 7.7 (standard deviation, 5.0). In the regression analysis, dental caries in permanent or deciduous teeth was positively associated with detectable viral load (adjusted odds ratio: 1.07, 95% confidence interval: 1.01–1.14). Conversely, antiretroviral therapy of ≥1 year and self-reported better adherence to antiretroviral drugs were negatively associated with detectable viral load. Among children with detectable viral load, dental caries in permanent or deciduous teeth was also positively associated with non-suppression of viral load (>1000 copies/mL) (adjusted odds ratio: 1.12, 95% confidence interval: 1.03–1.23).Conclusions: Dental caries was associated with viral load status detection among children living with HIV. This finding suggests that oral health status may affect the immune status of children. The oral health of children living with HIV should be strengthened, and further research is needed to clarify the causal relationship between viral load and oral health status.
Impact of relative dose intensity of oxaliplatin in adjuvant therapy among stage III colon cancer patients on early recurrence: a retrospective cohort study
Authors: Żok Jolanta , Bieńkowski Michał , Radecka Barbara , Korniluk Jan , Adamowicz Krzysztof , Duchnowska Renata 
Published: September 2020

Abstract
Background: Oxaliplatin-based therapy with FOLFOX-4 or CAPOX administered over 6 months remains the standard adjuvant treatment for stage III colon cancer (CC) patients. However, many patients experience dose reduction or early termination of chemotherapy due to oxaliplatin toxicity, which may increase the risk of early recurrence. The objective of this study was to analyze the relationship between the relative dose intensity of oxaliplatin (RDI-O) and early recurrence among stage III CC patients. Methods: The study included 365 patients treated at five oncology centers in Poland between 2000 and 2014.Survival analysis was performed using the Kaplan-Meier method. Univariate analysis was performed using the Cox proportional hazard model; multivariate analysis was performed with the stepwise forward approach. For all analyses the αlevel of 0.05 was employed. Results: The median follow-up was 51.8 months (range 8.2-115.1). Early recurrence <36 months after surgery occurred in 130 patients (37.8%). In this group 51 (39.2%) and 87 (66.9%) of patients were low and high-risk, respectively. Receipt <60% of RDI-O was associated with early recurrence within 18 months after surgery (OR=2.05; 95%CI: 1.18-3.51; p=0.010),especially in low-risk group (HR=1.56 (95%CI: 0.96-2.53), p=0.07). In the multivariate analysis early recurrence was correlated with grade (OR=2.47; 95% CI: 1.25-4.8; p=0.008), pN (OR=2.63; 95% CI: 1.55-4.54; p<0.001), the number of lymph nodes harvested (OR=0.51; 95% CI: 0.29-0.86; p=0.013) and RDI-O (OR=1.91; 95%CI: 1.06-3.39; p=0.028). The early vs. late recurrence negatively correlated with OS regardless of the RDI-O (HR=22.9 (95%CI: 13.9-37.6; p<0.001). Conclusions: RDI-O <60% in adjuvant therapy among stage III CC (especially in low-risk group) increases the risk of early recurrence within 18 months of surgery. Patients with early recurrence showed worse overall survival regardless of the RDI-O.
A full-length transcriptome and gene expression analysis reveal genes and molecular elements expressed during seed development in Gnetum luofuense
Authors: deng Nan , Hou Chen , He Boxiang , Ma Fengfeng , Song Qingan , Shi Shengqing , Liu Caixia , Tian Yuxin 
Published: September 2020

Abstract
Background: Gnetum is an economically important tropical and subtropical gymnosperm genus with various dietary, industrial and medicinal uses. Many carbohydrates, proteins and fibers accumulate during the ripening of Gnetum seeds. However, the molecular mechanisms related to this process remain unknown.Results: We therefore assembled a full-length transcriptome from immature and mature G. luofuense seeds using PacBio sequencing reads. We identified a total of 5,726 novel genes, 9,061 alternative splicing events, 3,551 lncRNAs, 2,160 transcription factors, and we found that 8,512 genes possessed at least one poly(A) site. In addition, gene expression comparisons of six transcriptomes generated by Illumina sequencing showed that 14,323 genes were differentially expressed from an immature stage to a mature stage with 7,891 genes upregulated and 6,432 genes downregulated. The expression of 14 differentially expressed transcription factors from the MADS-box, Aux/IAA and bHLH families was validated by qRT-PCR, suggesting that they may have important roles in seed ripening of G. luofuense.Conclusions: These findings provide a valuable molecular resource for understanding seed development of gymnosperms.
Water Loss During the Handwashing During COVID-19 Pandemic in Bangladesh
Authors: Sayeed Abu , Rahman Md Hafizur , Bundschuh Jochen , Herath Indika , Hasan M. Tasdik , Ahmed Fahad , Tariq Mohammad Raihan , Rahman Faujhia , Joy Md. Tarikul Islam , Abid Mohammad Tazrian , Saha Nondo 
Published: September 2020

Abstract
Handwashing is one of the vital public health measures to prevent COVID-19 pandemic from spread. However, water loss from excessive handwashing may put enormous pressure on the already overstretched groundwater resources and households’ economic well-being. Therefore, this study aimed to determine the water loss during handwashing amid the COVID-19 pandemic with an emphasis on the waste of groundwater from essential hygiene activities at domestic scale. Sociodemographic data were collected using a web-based survey tool among 1980 participants and an experiment was conducted among 126 voluntarily interested participants to estimate the amount of water wasted during handwashing. A total of 80% of the participants washed their hands regularly after returning home from outside. About 57.27% participants generally did not turn off their tap when washing their hands. A single participant, who keeps his tap on throughout the handwashing process, wasted approximately 1.7 L of water per handwash and 14.9 L of water per day. Handwashing when a tap is on, raised the water loss at 13-fold during this pandemic compared to the non-pandemic situation which cost extra 224.95 BDT (2.65$) per day for 1980 participants. A large number (57.27%) of people keep their faucet on during lathering hands with soap and scrubbing, thus wasting a considerable amount of water (1179% more compared to pre COVID-19 situation). The loss of water during handwashing is an alarming but less attentional issue. Behavioral change interventions are needed to aware people to shut off the faucet at periods when water is not being used.
Genome-wide characterization and expression analysis of the heat shock transcription factor family in pumpkin (Cucurbita moschata)
Authors: Shen Changwei , Yuan Jingping 
Published: September 2020

Abstract
Background: Crop quality and yield are affected by abiotic and biotic stresses, and heat shock transcription factors (Hsfs) are considered to play important roles in regulating plant tolerance under various stresses. To investigate the response of Cucurbita moschata to abiotic stress, we analyzed the genome of C. moschata. Results: In this research, a total of 36 C. moschata Hsf (CmHsf) members were identified and classified into three subfamilies (I, II, and III) according to their amino acid sequence identity. The Hsfs of the same subfamily usually exhibit a similar gene structure (intron-exon distribution) and conserved domains (DNA-binding and other functional domains). Chromosome localization analysis showed that the 36 CmHsfs were unevenly distributed on 18 of the 21 chromosomes (except for Cm_Chr00, Cm_Chr08 and Cm_Chr20), among which 18 genes formed 9 duplicated gene pairs that have undergone segmental duplication events. The Ka/Ks ratio showed that the duplicated CmHsfs have mainly experienced strong purifying selection. High-level synteny was observed between C. moschata and other Cucurbitaceae species. Conclusions: The expression profile of CmHsfs in the roots, stems, cotyledons and true leaves revealed that the CmHsfs exhibit tissue specificity. The analysis of cis-acting elements and quantitative real-time polymerase chain reaction (qRT-PCR) revealed that some key CmHsfs were activated by cold stress, heat stress, hormones and salicylic acid. This study lays the foundation for revealing the role of CmHsfs in resistance to various stresses, which is of great significance for the selection of stress-tolerant C. moschata.
Generation and molecular characteristics of a highly attenuated GPV strain through adaptation in GEF cells
Authors: Shao Hongxia , Jiang Yuchen , Yuan Huisha , Ji Lifei , Jin Wenjie , Qian Kun , Ye Jianqiang , Qin Aijian 
Published: September 2020

Abstract
Background: Goose parvoviruses (GPVs) spread globally and cause a huge economic loss to poultry industry. Although the attenuated GPV vaccines play a key role in preventing the disease caused by GPV, the molecular basis for the attenuation of GPV is barely known. Results: A highly attenuated GPV strain, GPV-CZM-142, was generated through blindly passaging of the highly pathogenic strain, GPV-CZM, in goose embryonic fibroblasts (GEF) for 142 generations. The virulence of the GEF-adapted GPV strain was 10000 times weaker than that of its wild type counterpart, GPV-CZM, based on the ELD50. By comparing with the wild type strain, genome sequencing analysis identified a novel insertion in ITR and several other mutations in both NS and VP1 of GPV-CZM-142.Conclusions: The highly attenuated GPV strain, GPV-CZM-142, provides a GPV vaccine candidate, and the identified virulence-related insertion/mutations give a novel insight into the molecular determinants of GPV virulence.
Ketamine-propofol (Ketofol) for Procedural Sedation and Analgesia in Children: A Systematic Review and Meta-Analysis
Authors: Foo Tze Yong , Noor Norhayati Mohd , Yazid Mohd Boniami , Fauzi Mohd Hashairi , Wahab Shaik Farid Abdull , Ahmad Mohammad Zikri 
Published: September 2020

Abstract
Objectives The aim of this review is to elucidate the efficacy and side effects of ketofol in comparison to other anaesthetic agents during procedural sedation and analgesia. Method The Cochrane Central Register of Controlled Trials (1996 to Feb 2019) and MEDLINE (1966 to Feb 2019) were searched, including the related randomised control trials and reviewed articles to find unpublished trials or trials not obtained via electronic searches. Inclusion criteria for the studies included comparing recovery time, recording clinician satisfaction, and assessing the adverse effects of ketofol. Results Eleven trials consisting of a total of 1274 patients met our criteria and were included in this meta-analysis. Five trials compared ketofol with a single agent, while six trials compared ketofol with combined agents. While comparing between ketofol and a single agent (either ketamine or propofol), ketofol showed significant effect on recovery time (MD: -9.88, 95% CI: -14.30 to -5.46; P=0.0003; I2=92%). However, no significant difference was observed while comparing ketofol with combined agents (RR: 0.75, 95% CI: -6.24 to 7.74; P<0.001; I2=98%). During single-agent comparison, ketofol showed no significant differences in terms of clinician satisfaction (RR: 2.86, 95% CI: 0.64 to 12.69; P=0.001; I2=90%), airway obstruction (RR: 0.72, 95% CI: 0.35 to 11.48; P=0.81; I2=0%), apnoea (RR: 0.9, 95% CI: 0.33 to 2.44; P=0.88; I2=0%), desaturation (RR: 1.11, 95% CI: 0.64 to 1.94; P=0.28; I2=21%), nausea (RR: 0.52, 95% CI: 0.91 to 1.41; P=0.2; I2=38%), and vomiting (RR: 0.63, 95% CI: 0.25 to 1.61; P=0.18; I2=42%). During comparison with combined agents, ketofol was more effective in reducing hypotension (RR: 4.2, 95% CI: 0.2 to 0.85; P=0.76; I2=0%), but no differences were observed in terms of bradycardia (RR: 0.70, 95% CI: 0.14 to 03.63; P=0.09; I2=53%), desaturation (RR: 1.9, 95% CI: 0.15 to 23.6; P=0.11; I2=61%), and respiratory depression (RR: 1.98, 95% CI: 0.18 to 21.94; P=0.12; I2=59%). Conclusion There is low certainty of evidence that ketofol improves recovery time and moderate certainty of evidence that it reduces the frequency of hypotension. There was no significant difference in terms of other adverse effects when compared to other either single or combined agents.Trial Registration PROSPERO CRD42019127278