bluuebunny/crossref_metadata_2025_split huggingface dataset:
Research on Multi-Factor Forest Fire Prediction Model Using Machine Learning Method in China
Authors: Li yudong , Feng Zhongke , Zhao Ziyu , Chen Shilin , Zhang Hanyue 
Published: August 2020

Abstract
Forest fires can cause serious harm in many ways. Studying the scientific prediction of forest fires is an important basis for preventing such fires. At present, there is little research on the prediction of long time series forest fires in China. Choosing a suitable forest fire prediction model is of great importance to China’s forest fire prevention and control work. Based on data on fire hotspots, meteorology, terrain, vegetation, infrastructure, and socio-economics collected from 2003 to 2016, we used a random forest model as a feature-selection method to determine 13 major drivers of forest fires in China (such as temperature, terrain etc.). The forest fire prediction models developed in this study are based on four machine-learning algorithms: an artificial neural network, a radial basis function network, a support-vector machine, and a random forest. The models were evaluated using the five performance indicators of accuracy, precision, recall, f1 value, and area-under-the-curve value. We used the optimal model to obtain the probability of forest fire occurrence in various provinces in China and create a spatial distribution map of the areas with high incidences of forest fires. The results show that the prediction accuracy of the four forest fire prediction models is between 75.8% and 89.2%, and the area-under-the-curve value is between 0.840 and 0.960. The random forest model has the highest accuracy (89.2%) and area-under-the-curve value (0.96). It is used as the optimal model to predict the probability of forest fire occurrence in China. The prediction results indicate that the areas with high incidences of forest fires are mainly concentrated in northeastern China (Heilongjiang Province and northern Inner Mongolia Autonomous Region), southeastern China (including Fujian Province and Jiangxi Province) etc. In those areas at high risk of forest fires, the management departments can improve the forest fire prevention and control by establishing watch towers and using other monitoring equipment. This study not only helps in understanding the main drivers of forest fires in China, but it also provides a reference for the selection of high-precision forest fire prediction models and provides a scientific basis for China’s forest fire prevention and control work.
MYCN Fish combined with immunohistochemistry is a more prognostic factor for neuroblastoma
Authors: Yang Yi , Zhao Jie , Zhang Yingwen , Yv Bo , Wang Jing , Feng Haizhong , Gao Yijin , Yin Minzhi , Tang Jingyan , Li Yanxin 
Published: August 2020

Abstract
Background: MYCN is an identified driver and important prognosticator of neuroblastoma and performs biological function at protein level. At present, most detection methodologies focus on MYCN DNA level and fail to detect MYCN protein expression for poor antibody, especially in China.Results：In the present study, through the analysis of neuroblastoma gene expression profile data, we found MYCN expression is closely related with prognosis of neuroblastoma, especially in stage I-III and IVs at RNA level in current medical conditions. FISH was almost accurate as WES to test MYCN DNA level based on our hospital data. In 56 neuroblastoma cases, IHC analysis by a newfound antibody (#84406s, Cell Signaling Technology) demonstrated a 75%-85% concordance with FISH. Further analyzing those cases with inconsistent results, we found patients with FISH+ but IHC score<9 all had no adverse events, while 63.6% patients with FISH+ and IHC score≥9 tended to have poor outcome. As to those MYCN FISH- patients, once their tumor showed MYCN protein expression, all of them were poor prognosis, while 81% of the rest patients with neither MYCN gene nor protein expression had ideal prognosis.Conclusions: The results suggest that this newfound antibody is reliable in IHC, and IHC combined with FISH performs better in evaluating prognosis and guiding treatment.
Comprehensive O-glycosylation analysis of the SARS-CoV-2 spike protein
Authors: Dong Xuefang , Chen Cheng , Yan Jingyu , Li Xiuling , Liang Xinmiao 
Published: August 2020

Abstract
The COVID-19 pandemic caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is a serious public health threat. Most vaccines being developed against SARS-CoV-2 target the highly glycosylated spike protein (S). A good knowledge of the glycosylation profile of this protein is key for successful vaccine development. Unlike the 22 confirmed N-glycosylation sites (NGSs) on the SARS-CoV-2 S, only a few O-glycosylation sites (OGSs) on this protein have been reported. This difference is mainly ascribed to an extremely low O-glycosylation stoichiometry. Herein, we comprehensively analyzed the O-glycosylation profile of recombinant SARS-CoV-2 S employing biomimetic polymer consisting of Trp-Arg monomer. Twenty-six OGSs and 33 O-linked glycans (OLGs) in the SARS-CoV-2 S were unambiguously identified, among which 24 OGSs and 25 OLGs are novel to the SARS-CoV-2 S. Our study reveals the comprehensive O-glycosylation profile of the SARS-CoV-2 S, which might shed light on viral pathobiology and assist in vaccine development.
Consistent Use of Condom and Associated Factors Among Sero-discordant Couples in Northeast Ethiopia, 2020:&nbsp;Qualitative Design Embedded
Authors: Ayele Wolde Melese , Tegegne Tesfaye Birhane , Damite Yitayish , Chane Muluken Genetu , Molla Asnakew 
Published: August 2020

Abstract
Background: Despite significant progress over the past decades to reduce transmission, HIV is continuing a public health problem. Although, consistent condom use has paramount help in reducing HIV transmission, its prevalence, and associated factors are not well studied. Therefore, this study was aimed to assess the proportion and factors associated with condom use among discordant couples in Northeast Ethiopia.Methods: An institution-based cross-sectional study by embedding qualitative design was conducted from October 1 2019 to June 2020. A total of 417 HIV discordant couples were included. A pre-tested structured and interviewer-administered questionnaire was used to collect the quantitative data. An interview guide unstructured questions were used to collect qualitative data. Binary logistic regression was employed to identify factors associated with the outcome variable. Variable those that had a P-value less than 0.05 in multivariable logistic regression were considered as statistical predictors of condom use. Results: A total of 401 HIV serodiscordant couples were participated, making a 94.8% response rate. The proportion of consistent condom use was 58.4%(95%CI: 53.1-63.1%). Unmarried partners AOR=0.44(95%CI: 0.229-0.877), students and employees AOR=0.33(95%CI 0.130-0.846), AOR=0.39(95%CI: 0.165-0.939) respectively, couples live together AOR=1.86(95%CI: 1.197-2.195), receiving counseling about condom use AOR=1.90(95%CI: 1.182-3.076), and knowledgeable participants AOR=1.61(95%CI: 1.031-2.525) were the independent predictors of consistent condom use. Conclusions: Despite its importance, the proportion of consistent condom use among serodiscordant couples was significantly low. Therefore, planners, policymakers, and health care practitioners should consider the aforementioned factors to improve consistent condom use. Also, special intervention on students and employees should be started. The authors recommend the researcher to quantify the new variable explored by qualitative design. Therefore, these results have high importance in designing tailored intervention method.
Immediate effects of using insoles with various wedges on plantar pressure measurements and comfort level during marching.
Authors: Shirvani Hossein , Shamsoddini Alireza , Ghasemi Mohamad Hosein 
Published: August 2020

Abstract
Background: Orthotic devices like insoles are widely used to improve performance. This study investigated whether immediate effects of using insoles with various wedges influence on plantar pressure measurements and comfort level during marching.Methods: Twenty-five (n=25) able-bodied males participated in this study voluntarily. Repeated measures analysis of variance test with a Bonferroni correction (P<0.0014) was used to compare dependent variables among nine insole wedge conditions.Results: The result of present study showed that the comfort level was non-significantly higher in the anterior and medial wedges compared to the posterior-lateral and anterior-lateral wedges during marching (P>0.0014). The peak of plantar pressure and impulse in the first to third metatarsal regions decreased in the anterior, medial and anterior-medial wedges compared to the lateral and posterior-lateral wedges (P<0.0014). In addition, a significant negative relation between the comfort level and plantar pressure measurements was observed in the second and third metatarsals and midfoot regions during marching (P<0.05).Conclusion: It seems that using anterior, medial and anterior-medial wedge for insole may have some advantage through increasing comfort sense and decreasing the risk of stress fracture injuries in the second and third metatarsal regions. These findings should be considered during designation of shoes or insoles for military environments.
Inflammaging: Analysis of a Risk Profile for Gerontocide by COVID-19 in Brazil
Authors: Neto Leônidas de Oliveira , Tavares Vagner Deuel de Oliveira , Agrícola Pedro Moraes Dutra , Oliveira Larissa Praça de , Sales Márcia Cristina , Sena-Evangelista Karine Cavalcanti Maurício de , Gomes Igor Conterato , Galvão-Coelho Nicole Leite , Pedrosa Lúcia Fátima Campos , Lima Kenio Costa de 
Published: August 2020

Abstract
Introduction: The increase in inflammatory cytokines associated with a reduction in the bioavailability of zinc has been used as a marker for inflammation. Despite the high inflammatory state found in institutionalized elderly individuals, few studies have proposed verifying the factors associated with this condition in this population to pandemic COVID-19.Objective: To verify the factors associated with inflamm-aging in institutionalized elderly.Methodology: A total of 187 elderly individuals (≥ 60 years old) living in the nursing homes of Natal/RN were included in the study. After cluster analysis was used to identify 3 groups according to their inflammatory state, an analysis of sarcopenia and anthropometric, biochemical, sociodemographic and health-related variables was performed. In sequence, an ordinal logistic regression was performed for a confidence level of 95% in those variables with p <0.20 in the bivariate analysis.Results: IL-6, TNFα and Zinc. Low-density lipids (LDL), high-density lipids (HDL) and triglycerides were associated with inflamm-aging. The increase of 1 unit of measurement of LDL, HDL and triglycerides increased the chance of inflammation-aging by 1.5%, 4.1% and 0.9%, respectively, while oldest old (≥80 years old) had 84.9% chance of presenting inflamm-aging in relation to no oldest old (<80 years).Conclusion: The association between biochemical markers and inflamm-aging demonstrates a relationship between endothelial injury and the inflammatory state. In addition, the presence of a greater amount of fat in the blood may present a higher relative risk of death from COVID-19.
Coagulopathy and damage control resuscitation during transfer due to massive hemorrhage –a single-center retrospective study–
Authors: Takahashi Hiroyuki , Shoko Tomohisa , Kojima Mitsuaki , Nara Satoshi 
Published: August 2020

Abstract
Background: To assess medical procedures, particularly damage control resuscitation, at the time of transfer between hospitals for the purpose of treating massive hemorrhage.Methods: This study used a single-center retrospective observational design, enrolling patients referred to Teine Keijinkai Hospital from another hospital between April 2012 and March 2019 for the treatment of massive hemorrhage. We excluded patients who entered cardiac arrest before arriving at our center. Qualitative or categorical variables were compared using the χ2 or Fisher’s exact test, as appropriate. Quantitative continuous variables were compared using Mann-Whitney nonparametric tests, as appropriate. Risk factors associated with coagulopathy from univariate analyses (fibrinogen level £150 mg/dl; prothrombin time-international normalized ratio ³1.5) were entered into stepwise logistic regression analysis. Significance was defined for values of p < 0.05.Results: Multiple logistic regression analysis revealed trauma (odds ratio (OR) 4.800; 95% confidence interval (CI) 2.016–11.433; p < 0.001) and volume of crystalloid solution (OR 1.001; 95%CI 1.000–1.001; p = 0.008) as independent factors associated with coagulopathy. Patients with coagulopathy showed higher 24-h mortality rates (10.9%) than patients without coagulopathy (1.2%; p = 0.021), and cause of death was hemorrhagic shock for all cases of death within 24 h.Conclusion: In our area, withholding intravenous fluid to achieve permissive hypotension, early administration of fresh frozen plasma, and use of fibrinogen concentrate may improve the prognosis of patients with massive hemorrhage undergoing transfer between hospitals.Trial registration: UMIN, UMIN000041201. Registered 24 July 2020 – Retrospectively registered, https://upload.umin.ac.jp/cgi-open-bin/icdr_e/ctr_view.cgi?recptno=R000047048
An analysis of medical malpractice lawsuits and its relationship to the growth of private hospitals
Authors: Sun Yu , Liu Gordon G. , Wang Mengxiao , Liu Sijia 
Published: August 2020

Abstract
Background Doctor-patient relationship has become an intense concern in public health in recent years. Medical malpractice lawsuits have presented an increasing trend over past decade. This study aims to describe distributions of medical malpractice lawsuits across different provinces and to investigate its relationship to the growth of private hospitals.Methods Data was drawn from China Judgments Online, the China Health Statistic Yearbooks and China Statistical Yearbooks between January 1, 2010 to December 31, 2016. Private hospitals expansion was measured by the number and proportion of private hospitals, the ratio of outpatient visits from private to public hospitals and the ratio of discharges from private to public hospitals. Fixed-effects negative binomial regressions were utilized to estimate the association between the growth of private hospitals and the number of medical malpractice lawsuits.Results In total, we identified a total of 18220 lawsuits relevant to medical malpractices across China between 2010 and 2016. The regression results revealed that more private hospitals, larger proportion of private hospitals and higher ratio of discharges from private to public hospitals were significantly associated with fewer medical malpractice lawsuits. An increase of 100 private hospitals in health care market was associated with a decrease in the number of medical malpractice lawsuits by 21%. The ratio of outpatient visits from private to public hospitals was not significantly associated with the number of malpractice lawsuits.Conclusions There was great disparities of incidences of medical malpractice lawsuits across different provinces. Greater expansion of private hospitals was associated with fewer medical malpractice lawsuits at province level. This negative association indicated that more private hospitals in health care market might provide more incentives for hospitals to contain the incidences of medical malpractice cases.
What are the Survival Factors in Surgically Resected Synchronous Multiple Primary Lung Cancers: A Retrospective Study
Authors: Li Haichao , Wang Kai , Zhang Xingxing , Chen Rong , Zhao Jian 
Published: August 2020

Abstract
Background: With the popularization of high-resolution computed tomography (HRCT), the detection rate of synchronous multiple primary lung cancer (SMPLC) is increasing. We retrospectively analyzed the surgical results of SMPLC patients in our hospital to determine the best treatment for SMPLC.Methods: A total of 90 SMPLC patients met the diagnostic criteria underwent complete resection and lymph node dissection or sampling without any preoperative induction therapy in the Department of Thoracic Surgery, Qilu Hospital, Cheeloo College of Medicine, Shandong University. We analyzed the postoperative survival rate, and further studied the relationship between survival rates and sex, age, preoperative symptoms, tumor location, tumor number, tumor size, surgical type, surgical frequency, histopathologic types, vascular infiltration, visceral pleural invasion and postoperative therapy.Results: Among 90 patients, the 1- and 3-year disease free survival (DFS) rates were 97.0% and 76.7% while the 1- and 3-year overall survival (OS) rates were 98.81% and 82.35%. Vascular infiltration (HR=402.46, p=0.005) and postoperative chemotherapy (HR>1000, p<0.001) were independent risk factors for DFS, while only postoperative chemotherapy (HR=184.10, p=0.002) was an independent risk factor for OS.Conclusions: First, SMPLC is different from intrapulmonary metastasis and its clinical stage is also different from the 8th (2015) edition TNM classification for lung cancer. Second, when pulmonary function permits, surgery (complete resection and lymph node dissection) is a significantly beneficial treatment for patients with SMPLC. Third, for early stage SMPLC patients, vascular infiltration and postoperative chemotherapy are harmful to the survival.
Assessment of Hydropower Potential Using Geospatial Technology in a Case Study of Guna-Tana Landscape Upper Abay Basin, Ethiopia
Authors: Teshome Asirat , Tibebu Yonas , Addis Endalkachew 
Published: August 2020

Abstract
Background: In this study, geospatial technology was used to assess potential sites for hydroelectric potential in the Ribb and Gumara Rivers of the Guna-Tana landscape of the upper Abay basin in Ethiopia. The important parameters used in this study were the Digital Elevation Model, the stream network, the stream elevation; the stream slope, the height difference, and the stream flow were analysed. In addition, the results obtained from the geospatial model, satellite data and GIS tools were used to identify the hydroelectric potential in the landscape. Results: Twenty sites with hydroelectric potential were identified within the 3528.16 km2 of the Guna-Tana landscape. The maximum power in the Ribb River was 48,389.98 kW, while in the Gumara River it was 41,984.01 kW. Therefore, the development of strategies to improve the decision-making process for hydroelectric power planning and construction is of utmost importance to support renewable energy production with minimal negative environmental social impacts. Conclusion: Therefore, this study revealed that the hydroelectric potential of a river basin could be correctly assessed using a digital elevation model, stream network data, within a GIS framework.
HOTAIR Promotes Cisplatin Resistance of Nasopharyngeal Carcinoma Cells by Regulating Cell Proliferation, Invasion, and Apoptosis via miR-106a-5p/SOX4 Axis
Authors: Cao Wei , Sun Yi , Liu Long , Yu Junwei , Ji Jiabiao , Wang Yatang , Yang Jianming 
Published: August 2020

Abstract
Background: Nasopharyngeal carcinoma (NPC) is a highly malignant neoplasm originating from nasopharyngeal mucosa, and the emergence of multi-drug resistance poses a huge challenge for clinical treatment of NPC. LncRNA HOTAIR (HOX antisense intergenic RNA) has been reported to be associated with many malignancies, including NPC. However, the underlying mechanisms of HOTAIR involved in drug resistance in NPC are obscure.Methods: Quantitative polymerase chain reaction (qPCR) was employed to determine the HOTAIR, miR-106a-5p and SOX4 expression in NPC tissues and cells. The target relationship between HOTAIR and miR-106a-5p or miR-106a-5p and SOX4 was determined using dual-luciferase reporter assay. Cell proliferation, apoptosis, migration and invasion were explored using Cell counting kit-8 (CCK-8), flow cytometer and Transwell assays. The protein levels were confirmed using western blot.Results: Our study showed that HOTAIR was upregulated in cisplatin (DDP)-resistant NPC tissues and cells. HOTAIR knockdown decreased the DDP resistance, drug resistance related gene expression, cell proliferation and invasion, and promoted apoptosis of C666-1/DDP and CNE2/DDP cells. Mechanism researches displayed that miR-106a-5p was down-regulated in DDP-resistant NPC tissues and cells. miR-106a-5p directly bound with HOTAIR and was regulated by HOTAIR. SOX4 was inhibited by miR-106a-5p at a posttranscriptional level, and the transfection of miR-106a-5p reversed the upregulation of SOX4 caused by HOTAIR overexpression. Increase or decrease of miR-106a-5p suppressed the effect of HOTAIR upregulation or downregulation on DDP resistance, cell proliferation, invasion and apoptosis of C666-1/DDP and CNE2/DDP cells. Moreover, the transfection of SOX4 siRNA reversed the decrease of DDP resistance, cell proliferation and invasion, and rescued the increase of apoptosis induced by miR-106a-5p inhibition. Conclusions: These data suggested that HOTAIR enhanced DDP resistance of C666-1/DDP and CNE2/DDP cells by affecting cell proliferation, invasion, and apoptosis via miR-106a-5p/SOX4 axis.
Screening of key genes and miRNAs involved in age-related hearing loss
Authors: song yin , zhang xue , sun wei jia , zhu ling jin , huang jin 
Published: August 2020

Abstract
Background

Age-related hearing loss (ARHL) is the most common sensory deficit and refers to the gradual loss of hearing function with age. We aimed to research the differential expression genes during the occur of ARHL and explore microRNAs that maybe regulate these genes.
Methods

Search the GEO data GSE6045, GSE35234, GSE62173, GSE45026 from NCBI's Gene Expression Omnibus database, and analyze in R. Normalize GEO data by RMA method in R, and use linear microarray data model (LIMMA) method in R to compare young and old mice to find differential genes and microRNAs.
Results

109 up-regulated and 121 down-regulated important genes were identified respectively. Functional enrichment shows that they are significantly enriched in protein digestion and absorption, neuroactive ligand-receptor interaction, and PI3K-Akt signaling pathway. Among the top 20 Hub genes, 7 down-regulated genes (Col3a1, Col1a2, Sparc, Col4a2, Col2a1, Lox, Sparc, Ctgf) have verified targeted miRNAs, which have interaction with differential miRNAs of GSE45026. Finally, we designated miR-29a-3p, miR-29b-3p, miR-29c-3p, and miR-124-3p as key miRNAs involved in the development of age-related hearing loss.
Conclusions

These low expression genes of Col3a1, Col1a2, Sparc, Col4a2, Col2a1,Lox, Sparc, and Ctgf maybe key genes of ARHL, and probably regulated by miR-29a-3p, miR-29b-3p, miR-29c-3p, and miR-124-3p.