bluuebunny/crossref_metadata_2025_split huggingface dataset:
Exercise intensity and physical fitness modulate lipoproteins profile during acute aerobic exercise session
Authors: Antunes B. M. , Rossi F. E. , Oyama L. M. , Rosa-Neto J. C. , Lira F. S. 
Published: March 2020

AbstractPhysical inactivity has emerged as an important cardiometabolic risk factor; however, the beneficial impacts of physical exercise according physical fitness status are still unclear. To analyze the lipoproteins and immune-endocrine response to acute aerobic exercise sessions performed at different intensities according physical fitness status and evaluated the gene expression in monocyte cells. Twelve individuals, divided into Low and High VO2max, performed three randomized acute exercise sessions at low (<60% VO2max), moderate (60–75% VO2max), and high (>90% VO2max) intensities. Blood samples were collected pre, immediately post, and 60 minutes post-exercise to analyze NEFA, triacylglycerol, non-HDL-c, HDL-c, PAI-1, leptin and adiponectin concentrations. Blood samples were collected from another set of twelve individuals for use in monocyte cell cultures to analyze L-CAT, CETP, and AMPK gene expressions. Low VO2max group pre-exercise exhibited higher postprandial leptin and total cholesterol concentrations than High VO2max group (p < 0.05). Exercise performed in high-intensity promoted a decreased leptin and NEFA levels (p < 0.05, for both), but for PAI-1 levels was decreased (p < 0.05) only for the Low VO2max group. Triacylglycerol levels decreased after all exercise sessions (p < 0.05) for both groups, and HDL-c exhibited decrease during moderate-intensity (p < 0.05), but this scenario was attenuated in Low VO2max group. Low VO2max individuals exhibit some metabolic-endocrine disruption, and acute aerobic exercise sessions performed at low, moderate, and high intensities are capable of modulating metabolic-endocrine parameters, mainly at high-intensity, in a physical fitness-dependent way, given that Low VO2max group was more responsive and seem to be able to appropriate more exercise-related benefits.
Evidence of trans-generational developmental modifications induced by simulated heat waves in an arthropod
Authors: Walzer A. , Formayer H. , Tixier M.-S. 
Published: March 2020

AbstractHeat waves are considered to pose a greater risk to arthropods with their limited thermoregulation abilities than the increase of mean temperatures. Theoretically, within- and trans-generational modifications may allow populations to keep pace with rapidly occurring heat waves. Here, we evaluated this assumption using individuals of predatory miteAmblydromalus limonicusfrom the F1 and F2 generation, which were exposed to summer or simulated heat wave conditions during juvenile development. Independent of generation, survival and male body size were insensitive to heat waves. Heat stress elongated juvenile development of F1 males and females, and lowered the F1 female size at maturity indicating non-adaptive within-generational effects. Trans-generational modifications speeded up the development of F2 males and females and resulted in larger body size of F2 females deriving from the heat wave-experienced F1 generation. Faster F2 development should be adaptive, because it reduces the exposure time to heat waves and promotes an early beginning of mating activities. Being large at extreme high temperatures maybe a benefit for the F2 females, because large individuals are less vulnerable to dehydration and overheating. Thus, the potential fitness loss from reduced F1 growth should be compensated by increased fitness in the F2 indicating adaptive trans-generational modifications.
Adapting SureSelect enrichment protocol to the Ion Torrent S5 platform in molecular diagnostics of craniosynostosis
Authors: Bukowska-Olech Ewelina , Popiel Delfina , Koczyk Grzegorz , Sowińska-Seidler Anna , Socha Magdalena , Wojciechowicz Bartosz , Dawidziuk Adam , Larysz Dawid , Jamsheer Aleksander 
Published: March 2020

AbstractObtaining reliable and high fidelity next-generation sequencing (NGS) data requires to choose a suitable sequencing platform and a library preparation approach, which both have their inherent assay-specific limitations. Here, we present the results of successful adaptation of SureSelect hybridisation-based target enrichment protocol for the sequencing on the Ion Torrent S5 platform, which is designed to work preferably with amplicon-based panels. In our study, we applied a custom NGS panel to screen a cohort of 16 unrelated patients affected by premature fusion of the cranial sutures, i.e. craniosynostosis (CS). CS occurs either as an isolated malformation or in a syndromic form, representing a genetically heterogeneous and clinically variable group of disorders. The approach presented here allowed us to achieve high quality NGS data and confirmed molecular diagnosis in 19% of cases, reaching the diagnostic yield similar to some of the published research reports. In conclusion, we demonstrated that an alternative enrichment strategy for library preparations can be successfully applied prior to sequencing on the Ion Torrent S5 platform. Also, we proved that the custom NGS panel designed by us represents a useful and effective tool in the molecular diagnostics of patients with CS.
Expansion of the Sahara Desert and shrinking of frozen land of the Arctic
Authors: Liu Ye , Xue Yongkang 
Published: March 2020

AbstractExpansion of the Sahara Desert (SD) and greening of the Arctic tundra-glacier region (ArcTG) have been hot subjects under extensive investigations. However, quantitative and comprehensive assessments of the landform changes in these regions are lacking. Here we use both observations and climate-ecosystem models to quantify/project changes in the extents and boundaries of the SD and ArcTG based on climate and vegetation indices. It is found that, based on observed climate indices, the SD expands 8% and the ArcTG shrinks 16% during 1950–2015, respectively. SD southern boundaries advance 100 km southward, and ArcTG boundaries are displaced about 50 km poleward in 1950–2015. The simulated trends based on climate and vegetation indices show consistent results with some differences probably due to missing anthropogenic forcing and two-way vegetation-climate feedback effect in simulations. The projected climate and vegetation indices show these trends will continue in 2015–2050.
Revealing the Hidden Details of Nanostructure in a Pharmaceutical Cream
Authors: Ahmadi Delaram , Mahmoudi Najet , Li Peixun , Ma Kun , Doutch James , Foglia Fabrizia , Heenan Richard K. , Barlow David , Lawrence M. Jayne 
Published: March 2020

AbstractCreams are multi-component semi-solid emulsions that find widespread utility across a wide range of pharmaceutical, cosmetic, and personal care products, and they also feature prominently in veterinary preparations and processed foodstuffs. The internal architectures of these systems, however, have to date been inferred largely through macroscopic and/or indirect experimental observations and so they are not well-characterized at the molecular level. Moreover, while their long-term stability and shelf-life, and their aesthetics and functional utility are critically dependent upon their molecular structure, there is no real understanding yet of the structural mechanisms that underlie the potential destabilizing effects of additives like drugs, anti-oxidants or preservatives, and no structure-based rationale to guide product formulation. In the research reported here we sought to address these deficiencies, making particular use of small-angle neutron scattering and exploiting the device of H/D contrast variation, with complementary studies also performed using bright-field and polarised light microscopy, small-angle and wide-angle X-ray scattering, and steady-state shear rheology measurements. Through the convolved findings from these studies we have secured a finely detailed picture of the molecular structure of creams based on Aqueous Cream BP, and our findings reveal that the structure is quite different from the generic picture of cream structure that is widely accepted and reproduced in textbooks.
Investigation of glass forming ability of Al-based metallic glasses by measuring vaporization enthalpy
Authors: Min Byeong-uk , Lee Jun-ho , Park Ho-jun , Jeon Gyu-tae , Jeong Jae Im , Lee Sung Hyuk , Kim Suk Jun 
Published: March 2020

AbstractThe analysis of the enthalpy changes for vaporization (ΔHvap) of Al-based metallic glass (MG) can provide insight into the origin of the MG’s glass forming ability (GFA). The ΔHvap of three Al-based MGs, Al84.5 ± x(Y10Ni5.5)15.5 ± x, Al85 ± x(Y8Ni5Co2)15 ± x, and Al86 ± x(Y4.5Ni6Co2La1.5)14 ± x, (hereafter referred to as AYNx, AYNCx, and AYNCLx, respectively), is analyzed by measuring their weight losses below their glass transition temperatures. The relationship between ΔHvap and aluminum concentration exhibit minimum values in the range of 83–85 at.% of Al, and the ΔHvap increases, becoming saturated at 320–350 kJ/mol, as the percentage of Al deviates from this range. The depth of the enthalpy well, referring to the bottom of the parabolic graph of ΔHvap against the Al concentration, is proportional to the viscosity of clusters showing liquid-like behavior. The amount of weight loss is proportional to the concentration of these clusters. The cluster viscosity and concentration influences the overall viscosity of the MGs, and thus determines the GFA.
Genetic and phenotypic landscape of the mitochondrial genome in the Japanese population
Authors: Yamamoto Kenichi , Sakaue Saori , Matsuda Koichi , Murakami Yoshinori , Kamatani Yoichiro , Ozono Keiichi , Momozawa Yukihide , Okada Yukinori 
Published: March 2020

AbstractThe genetic landscape of mitochondrial DNA (mtDNA) has been elusive. By analyzing mtDNA using the whole genome sequence (WGS) of Japanese individuals (n = 1928), we identified 2023 mtDNA variants and high-resolution haplogroups. Frequency spectra of the haplogroups were population-specific and were heterogeneous among geographic regions within Japan. Application of machine learning methods could finely classify the subjects corresponding to the high-digit mtDNA sub-haplogroups. mtDNA had distinct genetic structures from that of nuclear DNA (nDNA), characterized by no distance-dependent linkage disequilibrium decay, sparse tagging of common variants, and the existence of common haplotypes spanning the entire mtDNA. We did not detect any evidence of mtDNA–nDNA (or mtDNA copy number–nDNA) genotype associations. Together with WGS-based mtDNA variant imputation, we conducted a phenome-wide association study of 147,437 Japanese individuals with 99 clinical phenotypes. We observed pleiotropy of mtDNA genetic risk on the five late-onset human complex traits including creatine kinase (P = 1.7 × 10−12).
Fast room-temperature functionalization of silicon nanoparticles using alkyl silanols
Authors: van den Boom Alyssa F. J. , Pujari Sidharam P. , Bannani Fatma , Driss Hafedh , Zuilhof Han 
Published: March 2020

We report a fast, easy and efficient method for the functionalization of hydrogen-terminated silicon nanoparticles (H-Si NPs). Using silanol compounds, a range of functionalized Si NPs could be produced in only 1 h reaction time at room temperature.
Multi-omic analysis reveals HIP-55-dependent regulation of cytokines release
Authors: Jiang Yunqi , Xing Zihao , Zhu Baolin , Wang Wenjing , Sun Yang , Shi Zhi , Li Zijian 
Published: March 2020

Abstract
HIP-55 (HPK1 [hematopoietic progenitor kinase 1] -interacting protein of 55 kDa) contains an actin-depolymerizing factor homology (ADF-H) domain at the N-terminus and a src homology 3 (SH3) domain at the C-terminus, which plays an important role in the T cell receptor (TCR) and B-cell receptor (BCR) signaling and immune system. In our previous studies, HIP-55 was found to be highly expressed in several types of tumors and function as a novel oncogenic signaling hub that regulates tumor progression and metastasis through defined functional domains, actin-binding and SH3 modules. However, the wider functions and mechanisms of HIP-55 are still unclear. Here, multi-omic analysis revealed that one of the main biofunctions of HIP-55 is the regulation of cytokines release. Furthermore, to investigate the role of HIP-55 in the cytokine production, a series Cytokine Antibody Arrays were performed to detect differentially expressed cytokines between control and HIP-55 knockdown cells. A total of 97 differentially expressed cytokines were identified from 300 cytokines in A549 cell. Bioinformatics analysis showed these differentially cytokines were mainly enriched in cancer signal pathways and IL-6 is the most critical hub in the integrated network. Analysis of RNAseq data from lung cancer patients showed that there is a strong negative correlation between HIP-55 and interleukin-6 (IL-6) in samples from lung adenocarcinoma patients. Our data indicated that HIP-55 may participate in cancer progression and metastasis via regulating cytokines release.
Do We Have Effective Patient Education Materials for Age-Related Hearing Loss?
Authors: Squires Erika S. , Ou Hua 
Published: March 2020

PurposeThe purpose of this study was to explore and compare the readability and suitability of patient education materials (PEMs) on topics of age-related hearing loss (ARHL) supplied by electronic health record (EHR) systems and organizations specializing in communication sciences and disorders (CSD).MethodPEMs on ARHL were identified through a computerized search of EHR databases and CSD organization websites. Selected PEMs were assessed using three readability indices as well as the Suitability Assessment of Materials (SAM; Doak et al., 1996), which is a standardized tool to assess the content and design of written educational materials. Ten PEMs from EHR databases and 17 PEMs from CSD organizations were analyzed.ResultsOverall, 66.7% of PEMs were written above the eighth-grade readability target. PEMs from CSD organizations were significantly more difficult to read compared to those from EHR databases. In total, 85.2% of PEMs were classified as “adequate” using the SAM analysis. No significant SAM score differences were found between PEMs from CSD organizations and those from EHR databases. Common areas of weakness among PEMs were (a) failure to include a summary of key information, (b) reading level, (c) vocabulary (too advanced for the intended audience), and (d) limited subdivision of complex topics.ConclusionsThe readability and suitability of PEMs on topics of ARHL supplied by EHR providers and CSD organizations are not supportive of the health literacy skills of the average U.S. adult. It is critical to improve the readability, suitability, and comprehensibility of PEMs on ARHL to make information about hearing health care more accessible and usable.
Inner dusty envelope of the AGB stars W Hydrae, SW Virginis, and R Crateris using SPHERE/ZIMPOL
Authors: Khouri T. , Vlemmings W. H. T. , Paladini C. , Ginski C. , Lagadec E. , Maercker M. , Kervella P. , De Beck E. , Decin L. , de Koter A. , Waters L. B. F. M. 
Published: March 2020

Context. The asymptotic giant branch (AGB) marks the final evolutionary stage of stars with initial masses between ~0.8 and 8 M⊙. During this phase, stars undergo copious mass loss, which contributes significantly to the enrichment of the interstellar medium. The well-accepted mass-loss mechanism requires radiation pressure acting on dust grains that form in the density-enhanced and extended AGB stellar atmospheres. The details of the mass-loss process are not yet well understood, however. For oxygen-rich AGB stars, which are the focus of this study, the dust grains that drive the wind are expected to scatter visible light very efficiently because their sizes are relative large.
Aims. We study the distribution of dust in the inner wind of oxygen-rich AGB stars to advance our understanding of the wind-driving process.
Methods. We observed light scattered off dust grains that form around three oxygen-rich AGB stars (W Hya, SW Vir, and R Crt) with mass-loss rates between 10−7 and 10−6 M⊙ yr−1 using the extreme-adaptive-optics imager and polarimeter SPHERE/ZIMPOL with three filters centred at 0.65, 0.75 and 0.82 μm. We compared the observed morphologies and the spectral dependence of the scattered light between the three sources and determined the radial profile, per image octant, of the dust density distribution around the closest target, W Hya.
Results. We find the distribution of dust to be asymmetric for the three targets. A biconical morphology is seen for R Crt, with a position angle that is very similar to those inferred from interferometric observations of maser emission and of mid-infrared continuum emission. The cause of the biconical outflow cannot be inferred from the ZIMPOL data, but we speculate that it might be the consequence of a circumstellar disc or of the action of strong magnetic fields. The dust grains polarise light more efficiently at 0.65 μm for R Crt and SW Vir and at 0.82 μm for W Hya. This indicates that at the time of the observations, the grains around SW Vir and R Crt had sizes <0.1 μm, while those around W Hya were larger, with sizes ≳0.1 μm. The asymmetric distribution of dust around R Crt makes the interpretation more uncertain for this star, however. We find that polarised light is produced already from within the visible photosphere of W Hya, which we reproduce using models with an inner dust shell that is optically thick to scattering. We fit radiative transfer models to the radial profile of the polarised light observed around W Hya and find a steep dust density profile, with steepness varying considerably with direction. We find the wind-acceleration region of W Hya to extend to at least ~7 R⋆. This is in agreement with theoretical predictions of wind acceleration up to ~12 R⋆, and highlights that ZIMPOL observations probe the crucial region around AGB stars where dust forms and is accelerated.
Total Hip Arthroplasty for High Hip Dislocation
Authors: Hardt Sebastian , Hube Robert , Perka Carsten 
Published: March 2020

Abstract
Introduction Total hip arthroplasty in patients with high hip dislocation is a surgically demanding procedure. This is due to the congenital disorder of hip maturation and the resulting anatomical features. The aim of the arthroplasty is implantation of the cup prosthesis in the original centre of rotation, at the same time correcting femoral deformities and reducing the hip joint.
Indications The indications include advanced osteoarthritis in the secondary acetabulum, existing instability, severe contractures and secondary changes in adjacent joints.
Contraindications The contraindications include cerebrospinal dysfunction with impaired coordination, muscular dystrophies, overt metabolic bone disorders and complete or nearly complete absence of muscles that stabilise the hip/hip-stabilising musculature.
Operation Technique The operation is preferably performed through an anterolateral approach with the patient supine or through a posterolateral approach with the patient on his side. Sparing the pelvitrochanteric muscles is crucial for the functional outcome. Exposure of the original acetabulum is essential for correct and secure placement of the cup component. We perform planned shortening femoral osteotomy above an increase in length of more than 3.0 cm in patients without previous surgery and depending on the operative findings in previously operated patients. As a rule of thumb, the bone fragment to be removed should be approximately 60% of the distance between the planned and the preoperative position of the trochanter tip. Additional fixation is necessary only in the absence of primary stability between the parts of the femur divided by the osteotomy.
Postoperative Management In these patients, the postoperative management is determined individually depending on the stability of the prosthetic cup fixation, bone quality, rotational stability of the stem in both segments of the femur after the osteotomy, existing soft tissue contractures and the resulting postoperative leg length difference.
Complications The most frequent complications are nerve injuries, femoral fractures, malpositioning of the components, absence of integration of the components (usually due to inadequate primary stability), joint instability due to damage to the pelvitrochanteric muscles and therefore an increased risk of dislocation, pseudarthrosis of the femoral osteotomy and increased perioperative blood loss due to the prolonged operation time.
Results The currently published results show that subtrochanteric shortening osteotomy in patients with high hip dislocation with anatomic reconstruction of the original centre of rotation delivers good functional results with insignificantly increased 10-year loosening rates compared with standard management of primary hip osteoarthritis.
Optoakustische Bildgebung – Licht rein, Schall raus?
Authors: Hindelang B. , Schönmann C. , Aguirre J. , Ntziachristos V. , Biedermann T. , Darsow U. 
Published: March 2020

ZusammenfassungDie optoakustische Bildgebung ist eine neuartige Bildgebungsmodalität, welche auf der Aufzeichnung und Verarbeitung von Ultraschallsignalen basiert, die im Gewebe durch Absorption von Laserlicht entstehen. Die Methode vereint guten, auf Lichtabsorption beruhenden Kontrast mit hoher Eindringtiefe und ermöglicht sowohl morphologische als auch molekulare und funktionelle Bildgebung. Sie kann mit oder ohne Kontrastmittel eingesetzt werden und ist frei von ionisierender Strahlung. Darüber hinaus ist die Technik skalierbar und kann somit zur makroskopischen, mesoskopischen und mikroskopischen Bildgebung verwendet werden.In den letzten Jahren wurden eine Reihe verschiedener Systeme zur optoakustischen Bildgebung entwickelt und in vielfältigen Bereichen der präklinischen und klinischen Forschung eingesetzt. In der Dermatologie zeigten sich vielversprechende Anwendungsgebiete der Optoakustik insbesondere in der Untersuchung von Melanomen, Wächterlymphknoten und nicht-melanozytärem Hautkrebs sowie in der Charakterisierung von entzündlichen Hauterkrankungen.In diesem Review sollen die technischen Grundlagen der optoakustischen Bildgebung erläutert sowie der derzeitige Stand der Forschung hinsichtlich Anwendungsbereiche makroskopischer, mesoskopischer und mikroskopischer optoakustischer Systeme beschrieben und diskutiert werden.
Dermatofluoroskopie – Ist angeregtes Melanin anregend?
Authors: Fink C. 
Published: March 2020

ZusammenfassungDie Technologie der Dermatofluoroskopie beruht auf einer selektiven Anregung der Melaninfluoreszenz durch stufenweise 2-Photonen-Absorption. Hierbei wird das Melanin in einer Spektralregion angeregt, in der keine andere Hautkomponente absorbiert. Dadurch wird die Melaninfluoreszenz erstmals messbar. Unter Verwendung dieser Technik wird die Läsion Punkt für Punkt abgetastet. Die Fluoreszenzsignale werden dann mithilfe eines auf künstlicher Intelligenz basierten Algorithmus analysiert und das Ergebnis Score-basiert dargestellt. In Studien zeigte die Dermatofluoroskopie eine hohe diagnostische Genauigkeit mit guter Sensitivität und Spezifität. Das Medizinprodukt DermaFC® der Klasse IIa ist seit 2017 CE-zertifiziert und für den Einsatz als Diagnoseunterstützung bei melanozytären melanomverdächtigen Läsionen zugelassen. Nicht geeignet ist die Dermatofluoroskopie bei schwach pigmentierten Läsionen sowie schlecht zugänglichen Lokalisationen.