bluuebunny/crossref_metadata_2025_split huggingface dataset:
Gravitational and Finite‐Size Effects On Pressure Saturation Curves During Drainage
Authors: Ayaz Monem , Toussaint Renaud , Schäfer Gerhard , Måløy Knut Jørgen 
Published: September 2020

AbstractWe experimentally and numerically study the influence of gravity and finite‐size effects on the pressure‐saturation relationship in a given porous medium during slow drainage. The effect of gravity is systematically varied by tilting the system relative to the horizontal configuration. The use of a quasi two‐dimensional porous media allows for direct spatial monitoring of the saturation. Exploiting the fractal nature of the invasion structure, we obtain a relationship between the final saturation and the Bond number
using percolation theory. Moreover, the saturation, pressure, and Bond number are functionally related, allowing for pressure‐saturation curves to collapse onto a single master curve, parameterized by the representative elementary volume size and by the Bond and capillary numbers. This allows to upscale the pressure‐saturation curves measured in a laboratory to large representative elementary volumes used in reservoir simulations. The large‐scale behavior of these curves follows a simple relationship, depending on Bond and capillary numbers, and on the flow direction. The size distribution of trapped defending fluid clusters is also shown to contain information on past fluid flow and can be used as a marker of past flow speed and direction.
PolInSAR Coherence and Entropy‐Based Hybrid Decomposition Model
Authors: Shafai Shahid Shuja , Kumar Shashi 
Published: September 2020

AbstractTarget characterization is an essential aspect of polarimetric decomposition. This technique is capable of categorizing polarimetric signatures for different types of targets based on the scattering mechanisms they follow, enabling straightforward physical interpretation of the targets. The geometric anomalies associated with human‐made targets escalate the degree of randomness in the scattering process, which causes scattering ambiguity for such targets. The second‐order model descriptors do not relate to the actual physical structure and yield predominant volume scattering power. Such urban targets are decomposed as natural targets leading to irrelevant decomposition results. The methods developed to curb the problem are unable to maintain the consistency in the decomposition modeling as they underestimate volume scattering powers for natural landcover. A hybrid decomposition model is proposed herein to solve the problem of predominant volume scattering observed from urban targets by preserving volume scattering powers for natural targets. The model uses eigenvalue‐based decomposition parameters and polarimetric interferometric synthetic aperture radar (PolInSAR) coherence to decompose ambiguous targets. The proposed model has been tested on NISAR UAVSAR PolInSAR data acquired over the Greenville region, MS, USA. The proposed model has increased the double‐bounce scattering from the urban targets and enhanced the volume scattering from natural landcover as well. By comparing the results with existing decomposition models, it is observed that the proposed model gives a more robust representation of the landcover than the compared decomposition models.
What Controls Maximum Magnitudes of Giant Subduction Earthquakes?
Authors: Muldashev Iskander A. , Sobolev Stephan V. 
Published: September 2020

AbstractGiant earthquakes with magnitudes above 8.5 occur only in subduction zones. Despite the developments made in observing large subduction zone earthquakes with geophysical instruments, the factors controlling the maximum size of these earthquakes are still poorly understood. Previous studies have suggested the importance of slab shape, roughness of the plate interface contact, state of the strain in the upper plate, thickness of sediments filling the trenches, and subduction rate. Here, we present 2‐D cross‐scale numerical models of seismic cycles for subduction zones with various geometries, subduction channel friction configurations, and subduction rates. We found that low‐angle subduction and thick sediments in the subduction channel are the necessary conditions for generating giant earthquakes, while the subduction rate has a negligible effect. We suggest that these key parameters determine the maximum magnitude of a subduction earthquake by controlling the seismogenic zone width and smoothness of the subduction interface. This interpretation supports previous studies that are based upon observations and scaling laws. Our modeling results also suggest that low static friction in the sediment‐filled subduction channel results in neutral or moderate compressive deformation in the overriding plate for low‐angle subduction zones hosting giant earthquakes. These modeling results agree well with observations for the largest earthquakes. Based on our models we predict maximum magnitudes of subduction earthquakes worldwide, demonstrating the fit to magnitudes of all giant earthquakes of the 20th and 21st centuries and good agreement with the predictions based on statistical analyses of observations.
When There Is No Offset: A Demonstration of Seismic Diffraction Imaging and Depth‐Velocity Model Building in the Southern Aegean Sea
Authors: Preine J. , Schwarz B. , Bauer A. , Hübscher C. 
Published: September 2020

AbstractA vast majority of marine geological research is based on academic seismic data collected with single‐channel systems or short‐offset multichannel seismic cables, which often lack reflection moveout for conventional velocity analysis. Consequently, our understanding of Earth processes often relies on seismic time sections, which hampers quantitative analysis in terms of depth, formation thicknesses, or dip angles of faults. In order to overcome these limitations, we present a robust diffraction extraction scheme that models and adaptively subtracts the reflected wavefield from the data. We use diffractions to estimate insightful wavefront attributes and perform wavefront tomography to obtain laterally resolved seismic velocity information in depth. Using diffraction focusing as a quality control tool, we perform an interpretation‐driven refinement to derive a geologically plausible depth‐velocity model. In a final step, we perform depth migration to arrive at a spatial reconstruction of the shallow crust. Further, we focus the diffracted wavefield to demonstrate how these diffraction images can be used as physics‐guided attribute maps to support the identification of faults and unconformities. We demonstrate the potential of this processing scheme by its application to a seismic line from the Santorini Amorgos Tectonic Zone, located on the Hellenic Volcanic Arc, which is notorious for its catastrophic volcanic eruptions, earthquakes, and tsunamis. The resulting depth image allows a refined fault pattern delineation and, for the first time, a quantitative analysis of the basin stratigraphy. We conclude that diffraction‐based data analysis has a high potential, especially when the acquisition geometry of seismic data does not allow conventional velocity analysis.
Tropical Montane Forest Conversion Is a Critical Driver for Sediment Supply in East African Catchments
Authors: Stenfert Kroese Jaqueline , Jacobs Suzanne R. , Tych Wlodek , Breuer Lutz , Quinton John N. , Rufino Mariana C. 
Published: September 2020

AbstractLand use change is known to affect suspended sediment fluxes in headwater catchments. There is however limited empirical evidence of the magnitude of these effects for montane catchments in East Africa. We collected a unique 4‐year high‐frequency data set and assessed seasonal sediment variation, water pathways, and sediment response to hydrology in three catchments under contrasting land use in the Mau Forest Complex, Kenya's largest tropical montane forest. Annual suspended sediment yield was significantly higher in a smallholder agriculture‐dominated catchment (131.5 ± 90.6 t km−2 yr−1) than in a tea‐tree plantation catchment (42.0 ± 21.0 t km−2 yr−1) and a natural forest catchment (21.5 ± 11.1 t km−2 yr−1) (p < 0.05). Transfer function models showed that in the natural forest and the tea‐tree plantations subsurface flow pathways delivered water to the stream, while in the smallholder agriculture shallow subsurface and surface runoff were dominant. There was a delayed sediment response to rainfall for the smallholder agriculture and the tea‐tree plantations. A slow depletion in sediment supply suggests that the wider catchment area supplies sediment, especially in the catchment dominated by smallholder farming. In contrast, a fast sediment response and depletion in sediment supply in the natural forest suggests a dominance of temporarily stored and nearby sediment sources. This study shows that the vegetation cover of a forest ecosystem is very effective in conserving soil, whereas catchments with more bare soil and poor soil conservation practices generated six times more suspended sediment yield. Catchment connectivity through unpaved tracks is thought to be the main explanation for the difference in sediment yield.
OXPHOS-dependent metabolic reprogramming prompts metastatic potential of breast cancer cells under osteogenic differentiation
Authors: Hu Yangling , Xu Weimin , Zeng Hui , He Zilong , Lu Xiao , Zuo Daming , Qin Genggeng , Chen Weiguo 
Published: September 2020

Abstract
Background
Microcalcification is one of the most reliable clinical features of the malignancy risk of breast cancer, and it is associated with enhanced tumour aggressiveness and poor prognosis. However, its underlying molecular mechanism remains unclear.

Methods
Clinical data were retrieved to analyse the association between calcification and bone metastasis in patients with breast cancer. Using multiple human breast cancer cell lines, the osteogenic cocktail model was established in vitro to demonstrate calcification-exacerbated metastasis. Migration and invasion characteristics were determined by wound healing and transwell migration. mRNA and protein expression were identified by quantitative PCR and western blotting. Metabolic alterations in breast cancer cells were evaluated using Seahorse Analyser.

Results
The osteogenic differentiation of human breast cancer cells activated the classical TGF-β/Smad signalling pathway and the non-canonical MAPK pathway, which, in turn, exacerbated the progression of epithelial–mesenchymal transition (EMT). The metabolic programme switched to enhancing mitochondrial oxidative phosphorylation (OXPHOS) upon osteogenic differentiation. Rotenone was used to inhibit the OXPHOS complex during osteogenesis to block mitochondrial function, consequently reversing the EMT phenotype.

Conclusions
This study provides important insights into the mechanisms involved in breast cancer bone metastasis, and outlines a possible strategy to intervene in OXPHOS for the treatment of breast tumours.
Scheduling of nitrogen fertilizer topdressing during panicle differentiation to improve grain yield of rice with a long growth duration
Authors: Liu Yang , Zhu Xinguang , He Xiaoe , Li Chao , Chang Tiangen , Chang Shuoqi , Zhang Haiqing , Zhang Yuzhu 
Published: September 2020

AbstractTopdressing at panicle differentiation (PF) according to soil fertility and regularity of rice nutrient absorption is an important agronomic practice used in cultivation of rice cultivars with a long growth duration. We studied the impacts of timing of nitrogen fertilizer application during PF on photosynthesis and yield-related agronomic traits in ‘Y-Liang-You 900’ and ‘Y-Liang-You 6’, which are representative rice cultivars with a long growth duration. Data for two years showed that timing of topdressing application during PF affected panicles per unit area, percentage grain set, spikelets per panicle, and leaf photosynthetic traits during the grain-filling period. Topdressing at the initial stage of flag-leaf extension resulted in higher grain yield (typically by 10.55–19.95%) than in plants without topdressing. Grain yield was significantly correlated with flag leaf photosynthetic rate and leaf SPAD value (r = 0.5640 and r = 0.5589, respectively; p < 0.01) at an advanced grain-filling stage (30 days after heading). Surprisingly, grain yield was not correlated with carbohydrate remobilization from the stem and sheath. For rice cultivars with a long growth duration, nitrogen-fertilizer topdressing must be applied at the initial stage of flag-leaf extension to delay leaf senescence during the grain-filling stage and realize the enhanced yield potential.
Activity-based protein profiling of rice (Oryza sativa L.) bran serine hydrolases
Authors: Dolui Achintya Kumar , Vijayakumar Arun Kumar , Rajasekharan Ram , Vijayaraj Panneerselvam 
Published: September 2020

AbstractRice bran is an underutilized agricultural by-product with economic importance. The unique phytochemicals and fatty acid compositions of bran have been targeted for nutraceutical development. The endogenous lipases and hydrolases are responsible for the rapid deterioration of rice bran. Hence, we attempted to provide the first comprehensive profiling of active serine hydrolases (SHs) present in rice bran proteome by activity-based protein profiling (ABPP) strategy. The active site-directed fluorophosphonate probe (rhodamine and biotin-conjugated) was used for the detection and identification of active SHs. ABPP revealed 55 uncharacterized active-SHs and are representing five different known enzyme families. Based on motif and domain analyses, one of the uncharacterized and miss annotated SHs (Os12Ssp, storage protein) was selected for biochemical characterization by overexpressing in yeast. The purified recombinant protein authenticated the serine protease activity in time and protein-dependent studies. Os12Ssp exhibited the maximum activity at a pH between 7.0 and 8.0. The protease activity was inhibited by the covalent serine protease inhibitor, which suggests that the ABPP approach is indeed reliable than the sequence-based annotations. Collectively, the comprehensive knowledge generated from this study would be useful in expanding the current understanding of rice bran SHs and paves the way for better utilization/stabilization of rice bran.
Nanoscaled eutectic NiAl-(Cr,Mo) composites with exceptional mechanical properties processed by electron beam melting
Authors: Förner Andreas , Giese S. , Arnold C. , Felfer P. , Körner C. , Neumeier S. , Göken M. 
Published: September 2020

AbstractEutectic NiAl-(Cr,Mo) composites are promising high temperature materials due to their high melting point, excellent oxidation behavior and low density. To enhance the strength, hardness and fracture toughness, high cooling rates are beneficial to obtain a fine cellular-lamellar microstructure. This can be provided by the additive process of selective electron beam melting. The very high temperature gradient achieved in this process leads to the formation of the finest microstructure that has ever been reported for NiAl-(Cr,Mo) in-situ composites. A very high hardness and fracture toughening mechanisms were observed. This represents a feasibility study towards additive manufacturing of eutectic NiAl-(Cr,Mo) in-situ composites by selective electron beam melting.
Neurological disorder-associated genetic variants in individuals with psychogenic nonepileptic seizures
Authors: Leu Costin , Bautista Jocelyn F. , Sudarsanam Monica , Niestroj Lisa-Marie , Stefanski Arthur , Ferguson Lisa , Daly Mark J. , Jehi Lara , Najm Imad M. , Busch Robyn M. , Lal Dennis 
Published: September 2020

AbstractPsychogenic nonepileptic seizures (PNES) are diagnosed in approximately 30% of patients referred to tertiary care epilepsy centers. Little is known about the molecular pathology of PNES, much less about possible underlying genetic factors. We generated whole-exome sequencing and whole-genome genotyping data to identify rare, pathogenic (P) or likely pathogenic (LP) variants in 102 individuals with PNES and 448 individuals with focal (FE) or generalized (GE) epilepsy. Variants were classified for all individuals based on the ACMG-AMP 2015 guidelines. For research purposes only, we considered genes associated with neurological or psychiatric disorders as candidate genes for PNES. We observe in this first genetic investigation of PNES that six (5.88%) individuals with PNES without coexistent epilepsy carry P/LP variants (deletions at 10q11.22-q11.23, 10q23.1-q23.2, distal 16p11.2, and 17p13.3, and nonsynonymous variants in NSD1 and GABRA5). Notably, the burden of P/LP variants among the individuals with PNES was similar and not significantly different to the burden observed in the individuals with FE (3.05%) or GE (1.82%) (PNES vs. FE vs. GE (3 × 2 χ2), P = 0.30; PNES vs. epilepsy (2 × 2 χ2), P = 0.14). The presence of variants in genes associated with monogenic forms of neurological and psychiatric disorders in individuals with PNES shows that genetic factors are likely to play a role in PNES or its comorbidities in a subset of individuals. Future large-scale genetic research studies are needed to further corroborate these interesting findings in PNES.
Slow-growing broilers are healthier and express more behavioural indicators of positive welfare
Authors: Rayner Ann C. , Newberry Ruth C. , Vas Judit , Mullan Siobhan 
Published: September 2020

AbstractBroiler chicken welfare is under increasing scrutiny due to welfare concerns regarding growth rate and stocking density. This farm-based study explored broiler welfare in four conditions representing commercial systems varying in breed and planned maximum stocking density: (1) Breed A, 30 kg/m2; (2) Breed B, 30 kg/m2; (3) Breed B, 34 kg/m2; (4) Breed C, 34 kg/m2. Breeds A and B were ‘slow-growing’ breeds (< 50 g/day), and Breed C was a widely used ‘fast-growing’ breed. Indicators of negative welfare, behavioural indicators of positive welfare and environmental outcomes were assessed. Clear differences between conditions were detected. Birds in Condition 4 experienced the poorest health (highest mortality and post-mortem inspection rejections, poorest walking ability, most hock burn and pododermatitis) and litter quality. These birds also displayed lower levels of behaviours indicative of positive welfare (enrichment bale occupation, qualitative ‘happy/active’ scores, play, ground-scratching) than birds in Conditions 1–3. These findings provide farm-based evidence that significant welfare improvement can be achieved by utilising slow-growing breeds. There are suggested welfare benefits of a slightly lower planned maximum stocking density for Breed B and further health benefits of the slowest-growing breed, although these interventions do not offer the same magnitude of welfare improvement as moving away from fast-growing broilers.
Amelioration of cyclophosphamide-induced myelosuppression during treatment to rats with breast cancer through low-intensity pulsed ultrasound
Authors: Wang Wei , Luo Dong , Chen Junlin , Chen Jinyun , Xia Yi , Chen Wenzhi , Wang Yan 
Published: September 2020

Abstract
To investigate the alleviating effects of low-intensity pulsed ultrasound (LIPUS) on myelosuppression of Sprague–Dawley rats with breast cancer induced by cyclophosphamide (CTX). Breast cancer in rats was triggered by intragastric gavage with 7,12-dimethylbenz[a]anthracene (150 mg/kg). Then, the rats with breast cancer were randomly allocated to the LIPUS group (n=50) and the control group (n=50). The LIPUS group was injected intraperitoneally with CTX (50 mg/kg) for 4 consecutive days and underwent LIPUS treatment at femoral metaphysis 20 min per day from the first day of injection for 7 consecutive days. The control group was injected with CTX (50 mg/kg) and treated with LIPUS without energy output. Blood, enzyme-linked immunosorbent assay (ELISA), real-time quantitative polymerase chain reaction, Hematoxylin and Eosin (H&E) staining, and scanning electron microscopy were applied to detect the changes. The results indicated that LIPUS significantly promoted the proliferation of bone marrow nucleated cells, white blood cells (WBCs), IgA, IgG, and IgM in the peripheral blood (P&lt;0.05) without the damage to liver and kidney function simultaneously. The mechanisms may result from the LIPUS alleviation effect on bone marrow hematopoietic function through regulating cytokines such as LIPUS can increase the expression of granulocyte colony-stimulating factor (G-CSF), stem cell factor, transforming growth factor-β, and intercellular cell adhesion molecule-1, meanwhile LIPUS will decrease the expression of interleukin-6, tumor necrosis factor-α, and vascular cell adhesion molecule-1. LIPUS has potential to be a new adjuvant therapy method in clinic for ameliorating chemotherapy-induced myelosuppression.
GTF2IRD1 overexpression promotes tumor progression and correlates with less CD8+ T cells infiltration in pancreatic cancer
Authors: Zhuang Hongkai , Zhang Chuanzhao , Hou Baohua 
Published: September 2020

Abstract
Background: General Transcription Factor II-I Repeat Domain-Containing Protein 1 (GTF2IRD1) is a member of the GTF21 gene family, which encodes a set of multifunctional transcription factors. However, the potential function of GTF2IRD1 in pancreatic cancer (PC) still remains unknown. Study on GTF2IRD1 might provide a new insight into the carcinogenesis and therapeutics of PC.
Methods: In the current study, the clinical significance and potential biological of GTF2IRD1 were evaluated by bioinformatics analysis. The oncogenic role of GTF2IRD1 in PC was also determined using in vitro studies. Possible associations between GTF2IRD1 expression and tumor immunity were analyzed using ESTIMATE algorithm and single-sample Gene Set Enrichment Analysis (ssGSEA).
Results: GTF2IRD1 expression was significantly up-regulated in tumor tissues, and positively associated with higher histologic grade, higher American Joint Committee on Cancer (AJCC) stage, and worse prognosis. Function enrichment analysis demonstrated that GTF2IRD1 may be involved in pancreatic adenocarcinoma pathway, TGF-β signaling pathway, and tumor-infiltrating lymphocyte (TIL) related biological functions, such as T-cell receptor signaling pathway, leukocyte transendothelial migration, resistin as a regulator of inflammation, and regulation of leukocyte-mediated cytotoxicity. Knockdown of GTF2IRD1 expression inhibited cancer cell proliferation, colony formation, and invasion in vitro. ESTIMATE algorithm and ssGSEA demonstrated that GTF2IRD1 expression negatively correlated with the infiltration and anti-tumor activity of TILs, especially for CD8+ T cells.
Conclusion: The study demonstrates that GTF2IRD1 overexpression promotes tumor progression and correlates with less CD8+ T cells infiltration in PC.