bluuebunny/crossref_metadata_2025_split huggingface dataset:
Inequality, Chronic Undernutrition, Maternity, and Diabetes Mellitus as the Determinant of Anemia among Ever-Married Women in Bangladesh.
Authors: Islam GM 
Published: May 2020

Abstract
Purpose: This study aims todelineate the association between household economic inequalities, along with other confounders, and anemia among ever married women in Bangladesh.Methods: A cross-sectional population sample comprising of 5920 ever-married women aged between 15–49 years were selected for this Analysis were performed through the application of proportional odds models.Results: The respondents from low and medium socioeconomic status (SES) households vs. richest households were 1.90 (95% CI, 1.65-2.17; p<.01) and 1.41 (95 % CI, 1.12-1.78; p<.01) times more likely to suffer from anemia. The likelihood of being anemic reduces for the lowand medium SES groups vs. high SES group when sociodemographic variables are added (OR,1.69, 95% CI, 1.43-1.99 and OR, 1.35, 95% CI, .07-1.70;p<0.01 respectively). After adding the variables of diabetes and maternity status, the association betweenhaving anemia belongingto low and medium SES vs. high SESbecomes weaker (OR,1.36, 95% CI,0.85-2.15 and OR,1.15, 95% CI, 0.6-2.19; p>.05 respectively). Moreover, the strength of the association between anemia and the subjects with pregnant and lactating vs. the subjectswithout these conditionswas reduced after factoring body mass index (BMI).Conclusions: BMI, pregnancy, and diabetes mellitus were observed to be significantly associated with anemia and the strength of the association was low with anemia and socioeconomic inequality.
Cyclosporin A promotes invasion and migration of extravillous trophoblast cells derived from human induced pluripotent stem cells and human embryonic stem cells
Authors: Wang Jiaxing , Long Ping , Tian Shengnan , Zu Weihua , Liu Jing , Wu Bangyong , Mao Jilong , Li Dan , Ma Yanlin , Huang Yuanhua 
Published: May 2020

Abstract
Background

Extravillous trophoblast (EVT) cells play an essential role in the maternal-fetal interaction. Although abnormal development and function of EVT cells, including impaired migration and invasion capability, are believed to be etiologically linked to severe pregnancy disorders including pre-eclampsia (PE), the associated molecular mechanisms are not clear ascribed to the lack of an appropriate cell model in vitro. Cyclosporine A (CsA) is a macrolide immunosuppressant and is also used in clinic to improve pregnancy outcomes. However, whether CsA has any effects on the function of EVT cells has not been well investigated.
Methods

In this study, we induced differentiation of human induced pluripotent stem cells (hiPSCs) and human embryonic stem cells (hESCs) into EVT cells (hiPSC-EVT and hESC-EVT cells, respectively) by Y27632, NRG1, A83-01 and matrigel, and collected these derived EVT cells by flow cytometry for sorting cells positive for double HLA-G and KRT7, which are EVT markers. We then investigated the effects of CsA on the invasion and migration of these derived EVT cells.
Results

We found that the hiPSC-EVT and hESC-EVT cells expressed high levels of the EVT markers such as KRT7, ITGA5 and HLA-G but low levels of OCT4, a stem cell marker, and that CsA significantly promoted the invasion and migration of hiPSC-EVT and hESC-EVT cells.
Conclusions

We successfully generated hiPSC/hESC-derived human EVT cells, which may be applicable for investigating the remodeling process of spiral arteries remodeling and the possible mechanisms of EVT-related diseases in vitro. Furthermore, our findings provide direct evidence that CsA regulates the function of EVT cells and molecular basis by which CsA may be used to treat pregnancy complications in clinic associated with deficient EVT function.
Healthy Protection Of Bergamot Is Linked To The Modulation Of Microbiota
Authors: Riva Antonella , Longo Valeria , Berlanda Davide , Allegrini Pietro , Masetti Giulia , Botti Sara , Petrangolini Giovanna 
Published: May 2020

Abstract
Background: The present study aimed to evaluate the effects of a new food-grade bioavailable delivery system of bergamot on human gut microbiota, in order to demonstrate the potential correlation of microbiota modulation in cardiovascular health.The identification of human gut microbiota modification was performed after ex-vivo incubation with bergamot phytosome (Vazguard™) of individual faecal slurries from healthy women (45–53 years) as follows: after incubation at 37°C in anaerobic condition, DNA was extracted and a 16S Metagenomic Sequencing Analysis performed. Results: Twenty-five different phyla were identified, among which 4 were modulated: Firmicutes, Proteobacteria, Bacteroidetes, Actinobacteria. The decreased Firmicutes/Bacteroidetes ratio and the increase of Proteobacteria were observed indicating a positive modulation of microbiota possibly linked to cardiovascular health. 418 different genera were also identified, among which several of them were mildly modulated.Conclusions: For the first time, a gut microbiome modulation was associated to the new delivery system of bergamot phytosome, supporting its clinical efficacy for cardiovascular health. New potential applications in weight control and gastrointestinal benefits were suggested.
First molecular insights into the infection process provoked by Neofusicoccum parvum in Liquidambar styraciflua and the identification of new cysteine rich proteins in both organisms
Authors: Vázquez-Avendaño Rebeca , Rodríguez-Haas José Benjamín , Velázquez-Delgado Hugo , Rosas-Saito Greta Hanako , Hernández-Domínguez Eric Edmundo , Sánchez-Rangel Diana 
Published: May 2020

Abstract
Background Neofusicoccum parvum belongs to Botryosphaeriaceae family that groups endophytic and latent pathogens of woody plants responsible of diseases such as cankers, dieback and blight. Is a widespread pathogen in a broad host range including agricultural, horticultural and forestry plants, therefore is relevant to characterize molecular mechanisms involved in the disease. This work, report for first time a N. parvum as a pathogen of Liquidambar styraciflua. We established an in vitro pathosystem using foliar tissue in order to characterize the infection process through scanning electron microscopy. Because cysteine rich proteins (CysRPs) are well described for their important functions under plant-pathogen interaction, new CysRPs were identified for these organisms, and mRNAs expression of these proteins was analyzed at early times during the interaction.Results Since the first 24 hours post infection, the pathogen caused visible symptoms and the microscopic analysis at 16 days post infection revealed the presence of N. parvum pycnidium immersed in L. styraciflua leaf tissue. For both organisms, two databases with transcriptomic and genomic information were analyzed and new five CysRPs were identified for each organism, the amino acid length varied between 95 and 204 and in silico analysis revealed that all proteins are potentially secreted. The search of conserved domains and phylogenetic analyses bring to light that all proteins are novel including two of N. parvum that present the well-known CFEM domain. RT-qPCR analysis was conducted at 24- and 72-hours post infection and the results showed change levels of CysRPs mRNAs for both the plant and the fungus at early times during the interaction.ConclusionsIt is recognized for first time that N. parvum is a pathogen of L. styraciflua and this work represents an approach to begin to deeply understand the molecular mechanisms involved in this interaction highlighting the potential involvement of CysRPs of both organism during this biotic stress.
Outcomes in patients with lacrimal gland carcinoma treated with definitive radiotherapy or eye-sparing surgery followed by adjuvant radiotherapy
Authors: Lin Yun-Hsuan , Huang ShihMing , Yap Wing-Keen , Yang Ju-Wen , Yeung Ling , Tsan Din-Li , Chang Joseph Tung-Chieh , Chen Lung-Chien 
Published: May 2020

Abstract
The authors have withdrawn this preprint due to erroneous posting.
Flexible screen-printed temperature sensor based on Mn-Co-Ni metal oxide powder filled PVB polymer
Authors: Zhou Junyi , Chen Xuan , Zhang H.M. , Chang Aimin , Liu Ting 
Published: May 2020

Abstract
There has recently been renewed interest in wearable devices and electronic skin because of the demand in real-time monitoring of human body temperature. This work developed a flexible paper-based temperature sensor by screen printing technology. The sensing layer is composed of Mn-Co-Ni metal oxide powders filled with Polyvinyl butyral (PVB). The flexible temperature sensor shows extremely high sensitivity (3.14%° C− 1) at human body temperature (25 to 45° C). It also exhibits excellent durability (less than 0.25%) during the long-term aging tests, which indicates that the flexible temperature sensor has great potential in wearable devices and electronic skin.
Forest Fire Area Detection by Using Landsat-8 and Sentinel-2 Satellite Images: A Case Study in Mugla, Turkey
Authors: Kurnaz Bahadir , Bayik Caglar , Abdikan Saygin 
Published: May 2020

Abstract
Background: Forests have an extremely important place in the ecosystem in terms of ensuring social and environmental balance. The biggest danger for forests that have this importance is forest fires due to various reasons. It is extremely important to estimate the formation and behavior characteristics of fires in terms of combating forest fires. Using the satellite images obtained with the developing technology for this purpose provides great convenience in the detection of the fire areas and the severity of the fire affected. In this study, forest fire that occurred in the Zeytinköy region of Muğla province was investigated using remotely sensed images. According to the reference data provided by the General Directorate of Forestry (GDF), 425 hectares of area was destroyed by fire. In this study, it is aimed to extract burn scar by applying seven vegetation indexes on Sentinel-2 and Landsat-8 satellite images. Additionally, forest fire areas have been determined with the object-based classification technique. Results: As a result of the study, when the obtained results are compared with the values obtained from GDF, it is determined that object based analysis of Sentinel-2 provided the highest accuracy with 98.36% overall accuracy and 0.976 kappa statistics. Comparing the results of spectral indices of Sentinel-2 and Landsat-8, Sentinel-2 resulted better results in all indices. Among the indices RdNBR and dNDVI obtained better results than other indices with Sentinel-2 and Landsat-8, respectively. Conclusions: In general, it has been determined that Sentinel-2 data is more suitable than Landsat-8 satellite images for determining Turkish red pine forest fired areas. Red and near infrared based images can be used for rapid mapping of fired areas. The results also indicated that the indices provided by multi-temporal Sentinel-2 data can assist forest management for rapid monitoring of fire scars and also for evolution of reforestation after fire.
Time-varying gene expression network analysis for evolution of human prefrontal cortex
Authors: Cui Yuehua , Wang Huihui , Wu Yongqing , Sa Jian , Li Zhi , Cao Hongyan 
Published: May 2020

Abstract
Background : The prefrontal cortex (PFC) constitutes a large part of the human central nervous system and is essential for the normal social affection and executive function of humans and other primates. Despite ongoing research in this region, the evolution of interactions between PFC genes is still unknown, and there is a need to better understand changes in expression of age-related genes over the lifespan. To investigate the evolution of PFC gene interaction networks and further identify hub genes, we obtained time-series gene expression data of human PFC tissues from the Gene Expression Omnibus (GEO) database. A statistical model, loggle, was used to construct time-varying networks and explore the evolution of PFC gene networks over time. Several common network attributes were used to explore the evolution of PFC gene networks over time. The hub genes of different evolutionary stages were identified. At the same time, we explored several known KEGG pathways in PFC and the corresponding development patterns of central genes. Results : Network similarity analysis showed that the development of human PFC is divided into three stages, namely, fast development period, deceleration to stationary period, and destructive recession period. We identified some genes related to PFC evolution at these different stages, including genes involved in neuronal differentiation or synapse formation, genes involved in nerve impulse transmission, and genes involved in the development of myelin around neurons. Some of these genes are consistent with findings in previous reports. Pathway evolution analysis suggests that the axon guidance pathway has been most responsive during the evolution of PFC. Conclusions : This study clarified the evolutionary trajectory of the interaction between PFC genes, and proposed a set of candidate genes related to PFC development, which helps further study of human brain development at the genomic level supplemental to regular anatomical analyses. The analytical process used in this study, involving the loggle model, similarity analysis, and central analysis, provides a comprehensive strategy to gain novel insights into the evolution and development of brain networks in other organisms.
Prediction and Evolution of B Cell Epitopes of Surface Protein in SARS-CoV-2
Authors: Lon Jerome Rumdon , Bai Yunmeng , Zhong Bingxu , Cai Fuqaing , Du Hongli 
Published: May 2020

Abstract
Background The discovery of epitopes is helpful to the development of SARS-CoV-2 vaccine.Methods The sequences of the surface protein of SARS-CoV-2 and its proximal sequences were obtained by BLAST, the sequences of the whole genome of SARS-CoV-2 were obtained from the GenBank. Based on the NCBI Reference Sequence: NC_045512.2, the conformational and linear B cell epitopes of the surface protein were predicted separately by various prediction methods. Furthermore, the conservation of the epitopes, the adaptability and other evolutionary characteristics were also analyzed.Results 7 epitopes were predicted, including 5 linear epitopes and 2 conformational epitopes, one of the linear and one of the conformational were coincide. The epitope D mutated easily, but the other epitopes were very conservative and the epitope C was the most conservative. It is worth mentioning that all of the 6 dominated epitopes were absolutely conservative in nearly 1000 SARS-CoV-2 genomes, and they deserved further study.Conclusion The findings would facilitate the vaccine development, had the potential to be directly applied on the treatment in this disease, but also have the potential to prevent the possible threats caused by other types of coronavirus.
A novel prophylactic parachute ankle brace CPAB
Authors: Zhou Xi , Wu Di , Li Zhengyao , Wu Xiangdong , Yan Bin , Liang Leilei , He Yu , Liu Yong 
Published: May 2020

Abstract
Background: Comparing the effects of a self-designed and self-manufactured novel prophylactic ankle brace (Chinese parachute ankle brace, CPAB) and two ordinary ankle braces on the ankle joint during a half-squat parachute landing via biomechanical assessment.Methods: Twenty elite paratroopers were in four different conditions: no brace, elastic brace, semi-rigid brace, and CPAB. Each participant was instructed to jump off a platform with three different heights, 40 cm, 80 cm, and 120 cm, and land on the AMTI force plate in a half-squat posture. The vertical ground reaction forces (vGRF), joint angles, moments, powers, and works were calculated. After the experiment, every participant completed the questionnaires designed for this study.Result: Increasing the dropping height increased all of the parameters significantly (P<0.01), except for time to peak vGRF (T-PvGRF). Applying three braces can all slightly increase vGRF (P=0.237) and reduce T-PvGRF by 6-10 ms, as well as decrease the joint angles, velocities, and moments on the sagittal and coronal planes. Wearing CPAB and a semi-rigid brace more efficiently restricted dorsiflexion and inversion (P<0.05), and they both significantly reduced ankle work (t=5.107, P<0.01; t=3.331, P<0.01) and peak power (t=7.237, P<0.01; t=6.711, P<0.01) at 120 cm. The total scores from low-to-high were semi-rigid brace (19.20±2.99), elastic brace (21.91±3.25), and CPAB (23.37±3.08).Conclusion: The CPAB was more effective at restricting ankle joint motion on the coronal and sagittal planes than the other two prophylactic ankle braces. Therefore, the CPAB had the advantages of a novel appearance, high efficiency, and superior comfort, providing a reliable choice for parachute jumping and training in China.
Influence of graded levels of baobab oil seed cake on growth performance and enteric methane emissions in Savannah × Boer crossbreed yearling goats.
Authors: Saho Samkelo , Ikusika Olusegun O , Mpendulo Conference Thando , Mupangwa John , Moyo Davison 
Published: May 2020

Abstract
Background: The recent concern on the emission of methane from ruminant livestock that contributes to climate change has called for a search of feed ingredient that will mitigate the emission of this gas at the same time increase the growth performance of the animal. One of such feed ingredients could be baobab oil seed cake. This study therefore, investigate the effects of feeding graded levels of baobab oil seed cake (BOSC) on growth performance and enteric methane emissions of Savannah × Boer goats at yearling age.Methods: A total of 24 goats weighing 16.63 ± 3.639 kg were used in a completely randomized block design. The study was conducted for a period of 70 days, with an adaptation period of 14 days. The baobab oil seed cake was included in the diets at 0% (control diet), 15%, 30% and 45 % on a mass basis. The experimental diets were iso-nitrogenous and iso-caloric. The parameters measured include the average daily feed intake (ADFI), body weight gain (BWG), and average daily gain (ADG), feed conversion ratio (FCR), and enteric methane emissions using Laser methane detector (LMD).Results The inclusion of baobab oil seed cake (15%, 30%, and 45% BOSC) in goats’ diets significantly increased (P<0.05) (ADFI). Females in the current study gained more weight than males. It was observed that goats fed diets with 0% baobab oil cake emitted higher methane output (P<0.05). Methane emission decreased as the baobab inclusion levels in the diet increase (P<0.05). Female Savannah ´ Boer goat emitted more methane than male (P<0.05). Both sex and baobab oil cake inclusion levels contributed significantly to methane emissions individually and interactively. Conclusion: Inclusion of baobab oil seed cake in the diet improved growth performance and could also be used to mitigate methane production in Savannah × Boer crossbreed goats at a range of 15 to 45 % inclusion levels without causing any detrimental effects on the goats.’
Transcatheter Arterial Embolization with N-butyl-2 Cyanoacrylate Glubrans 2 for the Treatment of Acute Renal Hemorrhage Under Coagulopathic Condition
Authors: Gong Maofeng , He Xu , Zhao Boxiang , Kong Jie , Wang Tao , Gu Jianping , Su Haobo 
Published: May 2020

Abstract
Background:The efficacy and safety of transcatheter arterial embolization (TAE) using the N-butyl-2 cyanoacrylate (NBCA) Glubran2 in the treatment of acute renal hemorrhage (RH) under coagulopathic conditionsarestill no consensus.Methods: Between February 2014 and June 2019, 8 patients underwent TAE with the NBCA Glubran2 for acute RH under coagulopathic conditions. Coagulopathy was defined as abnormal values of prothrombin time and activated partial thromboplastin time and/or a reduced platelet count.Angiograms and medical records were retrospectively reviewed to determine technical/clinical success, complications and recurrent hemorrhage after TAE, and follow-up outcomes were assessed.Results: Of note, one patient presented with severe coagulopathy, and three presented with severe RH and hemodynamic instability. The NBCA Glubran2 was employed as a sole embolic material in sixpatients. In the remaining two patients, it was employed for secondary embolization.Under coagulopathic conditions, due to the use of the NBCA Glubran2, both technical success and clinical success for acute RH were achieved in all patients. Duringa mean follow-up time of 30.1 months (range, 3-84 months), neither persistent nor recurrent active hemorrhage required repeat endovascular or surgical treatment for hemostasis. No Glubran2related complications occurred mid-TAE pro-cedure. In addition, renal function information was available for all patients, and there was no significant difference between the serum creatinine levels [(83.8 ± 15.5) vs (85.8 ± 32.2) μmol/L] before and one week after Glubran2 embolization (p=0.89; CI, -34.5 to 30.5).Conclusions: The present findings suggest that TAE with the NBCA Glubran2 may be a safe alternative treatment for the management of RH under coagulopathic conditions. In particular, this method appears to be a potentially attractive alternative when con-ventional embolic materials fail in patients with ongoing hemodynamic instability or even under severe coagulopathic conditions.
Correlation between quantitative dynamic contrast-enhanced MRI parameters and molecular typing and related immune proteins in gastric cancer
Authors: Yan Liangliang , Gao Jianbo , Qu Jinrong , Zhang Hongkai , Lu Yanan , Zhang He , Xia Qingxin 
Published: May 2020

Abstract
Background

To analyze the correlation between quantitative dynamic contrast-enhanced MRI (DCE-MRI) parameters and molecular typing and related immune proteins in gastric cancer.
Methods

Forty-three patients confirmed as gastric adenocarcinoma by histopathology were enrolled in this prospective study. DCE-MRI were performed before surgery, and quantitative DCE parameters (Ktrans, Kep, Ve) were measured. The specimens were stained with biomarkers EBER-ISH, MLH1, PMS2, E-Cadherin, P53 and HER-2. According to the different dyeing results, they were divided into five molecular types and four HER-2 expression grades. The quantitative DCE parameters among five molecular types was compared using the ANOVA or Kruskal-Wallis test, and pairwise comparison was performed using the LSD test. The quantitative DCE parameters between positive and negative expressions of related immune proteins were compared using the Mann-Whitney U test. The correlations between quantitative DCE parameters and HER-2 expression grades were evaluated using Spearman rank correlation test.
Results

Among 43 cases, 3 cases were EBV-positive groups, 9 cases were MSI groups, 2 cases were aberrant E-Cadherin groups, 23 cases were aberrant P53 groups, and 6 cases were normal P53 groups. There were significant differences of quantitative DCE parameters in Ktrans and Kep among five molecular types (P < 0.05). The Ktrans value of Aberrant P53 group was lower than that of EBV-positive group, MSI group and Normal P53 group, the Kep value of E-Cadherin group was lower than that of EBV-positive group, and the Kep value of Aberrant P53 group was lower than that of EBV-positive group and MSI group, and all differences were statistically significant (all P < 0.05). Aberrant E-Cadherin group has the lowest Ktrans value (0.28/min) and Kep value (0.26/min) in five groups, respectively. There were no significant differences in quantitative DCE parameters between positive and negative expressions of all related immune proteins (all P > 0.05). There were no significant correlation between HER-2 expression grades and Ktrans, Kep, Ve values (r=-0.08, -0.03, -0.16, P = 0.63, 0.84, 0.31, respectively).
Conclusion

Quantitative DCE-MRI parameters can assess some molecular types of gastric cancer in a certain extent, and not assess related immune protein expressions, and not related to HER-2 expression grades.