bluuebunny/crossref_metadata_2025_split huggingface dataset:
Temperature‐mediated shifts in salamander transcriptomic responses to the amphibian‐killing fungus
Authors: Ellison Amy , Zamudio Kelly , Lips Karen , Muletz‐Wolz Carly 
Published: December 2019

AbstractLife processes of ectothermic vertebrates are intimately linked to the temperature of their environment, influencing their metabolism, reproduction, behaviour and immune responses. In amphibians infected by the generalist chytrid pathogen Batrachochytrium dendrobatidis (Bd), host survival, infection prevalence and infection intensity are often temperature‐ and/or seasonally dependent. However, the transcriptional underpinnings of thermal differences in infection responses remain unknown. Measuring the impact of temperature on host responses to infection is a key component for understanding climatic influences on chytrid disease dynamics. The Bd‐responsive gene pathways in frogs are well documented, but our understanding of salamander immune expression profiles during infection with chytrids remains limited. Here we characterize the transcriptomic responses of Plethodon cinereus using RNA sequencing by comparing skin and splenic gene expression of individuals uninfected, succumbing to Bd infection and naturally cleared of Bd infection at three temperatures. We suggest that amphibian temperature‐dependent susceptibility to Bd is probably driven by shifts in expression of the innate and adaptive immune axes. Our study shows increased expression of transcripts associated with inflammation at lower temperatures and a shift towards increased expression of adaptive immune genes, including MHC (major histocompatibility complex), at higher temperatures. In the face of climate change, and as concerns for the spread of emergent chytrid pathogens increase, our results provide important functional genomic resources to help understand how these pathogenic fungi may continue to affect amphibian communities globally in the future.
Disruption of miRNA sequences by TALENs and CRISPR/Cas9 induces varied lengths of miRNA production
Authors: Bi Honghao , Fei Qili , Li Riqing , Liu Bo , Xia Rui , Char Si Nian , Meyers Blake C. , Yang Bing 
Published: December 2019

SummaryMicroRNAs (miRNAs) are 20‐24 nucleotides (nt) small RNAs functioning in eukaryotes. The length and sequence of miRNAs are not only related to the biogenesis of miRNAs but are also important for downstream physiological processes like ta‐siRNA production. To investigate these roles, it is informative to create small mutations within mature miRNA sequences. We used both TALENs (transcription activator‐like effector nucleases) and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR‐associated protein 9 (Cas9) to introduce heritable base pair mutations in mature miRNA sequences. For rice, TALEN constructs were built targeting five different mature miRNA sequences and yielding heritable mutations. Among the resulting mutants, mir390 mutant showed a severe defect in the shoot apical meristem (SAM), a shootless phenotype, which could be rescued by the wild‐type MIR390. Small RNA sequencing showed the two base pair deletion in mir390 substantially interfered with miR390 biogenesis. In Arabidopsis, CRISPR/Cas9‐mediated editing of the miR160* strand confirmed that the asymmetric structure of miRNA is not a necessary determinant for secondary siRNA production. CRISPR/Cas9 with double‐guide RNAs successfully generated mir160a null mutants with fragment deletions, at a higher efficiency than a single‐guide RNA. The difference between the phenotypic severity of miR160a mutants in Col‐0 versus Ler backgrounds highlights a diverged role for miR160a in different ecotypes. Overall, we demonstrated that TALENs and CRISPR/Cas9 are both effective in modifying miRNA precursor structure, disrupting miRNA processing and generating miRNA null mutant plants.
Validation of the Japanese Transition Readiness Assessment Questionnaire
Authors: Sato Yuki , Ochiai Ryota , Ishizaki Yuko , Nishida Toshihiko , Miura Kenichiro , Taki Atsuko , Tani Yumi , Naito Mariko , Takahashi Yoshimitsu , Yaguchi‐Saito Akiko , Hattori Motoshi , Nakayama Takeo 
Published: December 2019

AbstractBackgroundFor patients with childhood‐onset chronic illnesses, the transition to adult care requires an understanding of transition readiness and the effectiveness of evaluation methods. However, no such psychometrically verified scales exist in Japan. This study aimed to develop a Japanese version of the Transition Readiness Assessment Questionnaire (TRAQ) and verify its validity and reliability.MethodsThe Japanese TRAQ was developed in accordance with international guidelines, followed by a preliminary survey to verify face validity among six participants who fulfilled the inclusion criteria. For the main survey 107 patients who fulfilled the same inclusion criteria were asked to complete the questionnaire and provide basic information. After descriptive statistics analysis, the construct validity of the Japanese TRAQ was tested using the t‐test and Pearson's correlation coefficients. Cronbach's alpha coefficients were calculated to assess reliability.ResultsIn the main survey, 76 participants with no missing data were included in the complete data analysis (40 males 36 females; mean age, 17.8 and 18.2 years, respectively). The mean total Japanese TRAQ score was 3.9. Cronbach's alpha coefficients were 0.94 overall and 0.8–0.96 for each of the four domains. The known‐groups analysis revealed that older participants (r = 0.23, P = 0.044), those having knowledge of the disease name (yes [4.0] vs no [3.4]; P < 0.001), and making unaccompanied hospital visits (with parent/others [3.7] vs alone [4.4]; P < 0.001) had significantly higher total TRAQ scores.ConclusionWe confirmed preliminarily the validity and reliability of the Japanese TRAQ.
Assessment of rejection risk following subtherapeutic calcineurin inhibitor levels after pediatric heart transplantation
Authors: Kerr Sarah M. , Jorgensen Neal W. , Hong Borah J. , Friedland‐Little Joshua M. , Albers Erin L. , Newland David M. , Law Yuk M. , Kemna Mariska S. 
Published: December 2019

AbstractCNIs are the mainstay of immunosuppressive therapy after pediatric HTx. While regular laboratory surveillance is performed to ensure blood levels are within targeted range, the risk of acute rejection associated with subtherapeutic CNI levels has never been quantified. This is a retrospective single‐center review of 8413 CNI trough levels in 138 pediatric HTx recipients who survived >1 year after HTx. Subtherapeutic CNI levels were defined as <50% of the lower limit of target range. The risk of acute, late (>12 months post‐transplant) rejection following recipients' subtherapeutic CNI levels was assessed using time‐varying multivariable Cox proportional hazards analysis. We found that 79 of 138 recipients (57%) had at least one subtherapeutic CNI level on routine surveillance laboratories during a mean follow‐up of 5.5 ± 3.6 years. Following an episode of subtherapeutic levels, 17 recipients (22%) had biopsy‐proven rejection within the next 3 months; the majority (9/17) within the first 2 weeks. After presenting with subtherapeutic CNI levels, recipients incurred a 6.1 times increased risk of acute rejection in the following 3 months (HR = 6.11 [2.41, 15.51], P = <.001). Age at HTx, HLA sensitization, or positive crossmatch were not associated with acute late rejection, but rejection in the first post‐transplant year was (HR 2.61 [1.27, 5.35], P = .009). Thus, maintaining therapeutic CNI levels is the most important factor in preventing acute rejection in recipients who are >12 months after pediatric HTx. Recipients who present with subtherapeutic CNI levels on surveillance monitoring are 6.1 times more likely to develop rejection in the following 3 months.
Tacrolimus blood concentration increase depends on administration route when combined with voriconazole in pediatric stem cell transplant recipients
Authors: Utano Tomoyuki , Kato Motohiro , Osumi Tomoo , Shioda Yoko , Kiyotani Chikako , Terashima Keita , Tomizawa Daisuke , Matsumoto Kimikazu , Yamatani Akimasa 
Published: December 2019

AbstractBackgroundUnderstanding of TAC pharmacokinetics is required to avoid both overdosing and underdosing. VRCZ is known to increase the TAC blood concentration by inhibiting CYP3A4; however, detailed, practical information on pediatric cases is still scarce. Herein, we investigated the association between the TAC blood concentration and dosage focusing on the administration route and concomitant use of VRCZ in children.MethodsIn total, 38 children who received TAC during stem cell transplantation at our hospital between January 2013 and April 2018 were included. The ratio of the TAC blood concentration (ng/mL) to dosage (mg/kg/day) (C/D) was calculated at the last continuous intravenous infusion (C/Div) and after switching to oral administration (C/Dpo).ResultsPatients with VRCZ (n = 14) showed a higher C/D regardless of administration route (median C/Div: with VRCZ/without VRCZ = 832/643, median C/Dpo: with VRCZ/without VRCZ = 339/45). Additionally, the (C/Div)/(C/Dpo) was about one‐fourth in cases with VRCZ; the median (C/Div)/(C/Dpo) was 3.3 for cases with VRCZ and 13.5 for cases without VRCZ. Interestingly, the increase in the TAC blood concentration due to VRCZ was higher when TAC was administered orally, especially in adolescent patients.ConclusionsTo obtain an optimal TAC blood concentration, dose adjustment based on multiple factors, such as administration route, concomitant use of VRCZ, and age, is required.
André Naud: From Vatican II to Simone Weil
Authors: Schmidt Lawrence 
Published: December 2019

AbstractAndré Naud was a French‐Canadian Catholic theologian who served as a peritus or advisor to Cardinal Leger, the Archbishop of Montreal at the Second Vatican Council between 1962 and 1965. Naud’s entire theological career was informed by the teachings of the Council. This was the reason why during the Papacy of John Paul II after 1978 he became alarmed at the expansion and the distortion of the authority of the magisterium. Over the last fifteen years of his life, he wrote three remarkable books on this theme. In the last book (2002) entitled Les Dogmes et le Respect de l’Intelligence: Plaidoyer Inspiré par Simone Weil (The Dogmas and the Respect for Intelligence: An Argument Inspired by Simone Weil), he undertook an in‐depth analysis of Simone Weil, who had argued in the 1940s that Catholicism was in need of a philosophical cleaning up. This paper elaborates and explains the particular ways that Weil’s writings helped Naud to reformulate in the spirit of the Second Vatican Council the role of the magisterium with regard to dogmatic formulations, the manner of the reception of that teaching, the inspiration of scripture, the interpretation of miracles and the understanding of the Church itself.
Perception of audiovisual infant directed speech
Authors: Englund Nunne , Behne Dawn M. 
Published: December 2019

Infant perception often deals with audiovisual speech input and a first step in processing this input is to perceive both visual and auditory information. The speech directed to infants has special characteristics and may enhance visual aspects of speech. The current study was designed to explore the impact of visual enhancement in infant‐directed speech (IDS) on audiovisual mismatch detection in a naturalistic setting. Twenty infants participated in an experiment with a visual fixation task conducted in participants’ homes. Stimuli consisted of IDS and adult‐directed speech (ADS) syllables with a plosive and the vowel /a:/, /i:/ or /u:/. These were either audiovisually congruent or incongruent. Infants looked longer at incongruent than congruent syllables and longer at IDS than ADS syllables, indicating that IDS and incongruent stimuli contain cues that can make audiovisual perception challenging and thereby attract infants’ gaze.
“We're the Normal Ones Here”: Community Involvement, Peer Support, and Transgender Mental Health
Authors: Johnson Austin H. , Rogers Baker A. 
Published: December 2019

This article uses ethnographic methods to explore how peer support and community involvement influence the mental health and well‐being of transgender (or, trans) people in the southeastern United States. The study builds on existing research that suggests that trans community involvement and peer support among trans people enhance mental health experiences and moderate the effects of stigma and discrimination on health outcomes. Through qualitative analysis of 158 hours of participant observation and 33 in‐depth interviews with members of a trans community organization in the U.S. Southeast, this paper identifies three key processes through which peer support and community involvement enhance the mental health and well‐being of trans people: (1) the normalization of trans identities and experiences; (2) the creation of a social support network; and (3) the empowerment of trans people.
Frontline implementation of welfare conditionality in Norway: A maternalistic practice
Authors: Gjersøe Heidi Moen , Leseth Anne , Vilhena Susana 
Published: December 2019

AbstractWelfare conditionality is both ambitious and ambiguous for the frontline workers who put policy into practice. Since January 2017, the Norwegian frontline service should require social assistance benefit recipients under the age of 30 to participate in some sort of work‐related activation, so‐called mandatory activation. Drawing on qualitative interviews with frontline workers at local offices in the Norwegian Public Welfare Service (NAV), we investigate how the requirement is implemented in a context of a professionalised social welfare service. Mandatory activation is arguably a paternalistic measure. Drawing on Bernardo Zacka's concept of moral dispositions and Laura Specker Sullivan's concepts of maternalism, our findings indicate that at the frontline, mandatory activation policies are implemented by maternalistic decision making, emphasising the interpersonal relation between trained caseworkers and clients. The caseworkers use their discretionary powers in the implementation of conditionality and sanctions by emphasising care and support as embedded in the strict rules.
Combined molecular and morphological data provide insights into the evolution and classification of Chilocorini ladybirds (Coleoptera: Coccinellidae)
Authors: Li Wenjing , Łączyński Piotr , Escalona Hermes E. , Eberle Jonas , Huo Lizhi , Chen Xiaosheng , Huang Weidong , Chen Bingxu , Ahrens Dirk , Ślipiński Adam , Tomaszewska Wioletta , Wang Xingmin 
Published: December 2019

AbstractLadybirds of the cosmopolitan tribe Chilocorini prey mainly on coccids and include several important biocontrol agents. The phylogenetic relationships of Chilocorini are poorly known. In this paper, we provide a phylogenetic reconstruction of Chilocorini containing all 27 genera based on five molecular markers and 86 adult morphological characters. Morphological character states were mapped on the combined data tree from Bayesian inference to analyse morphological traits of each genus. Sixteen morphological characters were selected to reconstruct the ancestral states using maximum parsimony and maximum likelihood methods. Divergence times were estimated based on the relaxed molecular clock approach. Our results indicate that Chilocorini, excludingChilocorellusMiyatake, is monophyletic and closely related to Plotinini. The crown group Chilocorini was estimated to date back to the Middle Cretaceous.AnisorcusCrotch,EgiusMulsant,PhaenochilusWeise andSimmondsiusAhmad & Ghani are synonymized here withChilocorusLeach (syn.n.). The genusChilocorellusis excluded from Chilocorini. The split of current genera was estimated to have occurred during the Middle Paleogene to Late Paleogene.
Meiotic recombination profiling of interspecific hybrid F1 tomato pollen by linked read sequencing
Authors: Rommel Fuentes Roven , Hesselink Thamara , Nieuwenhuis Ronald , Bakker Linda , Schijlen Elio , van Dooijeweert Willem , Diaz Trivino Sara , de Haan Jorn R. , Sanchez Perez Gabino , Zhang Xinyue , Fransz Paul , de Jong Hans , van Dijk Aalt D. J. , de Ridder Dick , Peters Sander A. 
Published: December 2019

SummaryGenome wide screening of pooled pollen samples from a single interspecific F1 hybrid obtained from a cross between tomato, Solanum lycopersicum and its wild relative, Solanum pimpinellifolium using linked read sequencing of the haploid nuclei, allowed profiling of the crossover (CO) and gene conversion (GC) landscape. We observed a striking overlap between cold regions of CO in the male gametes and our previously established F6 recombinant inbred lines (RILs) population. COs were overrepresented in non‐coding regions in the gene promoter and 5′UTR regions of genes. Poly‐A/T and AT rich motifs were found enriched in 1 kb promoter regions flanking the CO sites. Non‐crossover associated allelic and ectopic GCs were detected in most chromosomes, confirming that besides CO, GC represents also a source for genetic diversity and genome plasticity in tomato. Furthermore, we identified processed break junctions pointing at the involvement of both homology directed and non‐homology directed repair pathways, suggesting a recombination machinery in tomato that is more complex than currently anticipated.
Evolutionary trends in RuBisCO kinetics and their co‐evolution with CO2 concentrating mechanisms
Authors: Iñiguez Concepción , Capó‐Bauçà Sebastià , Niinemets Ülo , Stoll Heather , Aguiló‐Nicolau Pere , Galmés Jeroni 
Published: December 2019

SummaryRuBisCO‐catalyzed CO2 fixation is the main source of organic carbon in the biosphere. This enzyme is present in all domains of life in different forms (III, II, and I) and its origin goes back to 3500 Mya, when the atmosphere was anoxygenic. However, the RuBisCO active site also catalyzes oxygenation of ribulose 1,5‐bisphosphate, therefore, the development of oxygenic photosynthesis and the subsequent oxygen‐rich atmosphere promoted the appearance of CO2 concentrating mechanisms (CCMs) and/or the evolution of a more CO2‐specific RuBisCO enzyme. The wide variability in RuBisCO kinetic traits of extant organisms reveals a history of adaptation to the prevailing CO2/O2 concentrations and the thermal environment throughout evolution. Notable differences in the kinetic parameters are found among the different forms of RuBisCO, but the differences are also associated with the presence and type of CCMs within each form, indicative of co‐evolution of RuBisCO and CCMs. Trade‐offs between RuBisCO kinetic traits vary among the RuBisCO forms and also among phylogenetic groups within the same form. These results suggest that different biochemical and structural constraints have operated on each type of RuBisCO during evolution, probably reflecting different environmental selective pressures. In a similar way, variations in carbon isotopic fractionation of the enzyme point to significant differences in its relationship to the CO2 specificity among different RuBisCO forms. A deeper knowledge of the natural variability of RuBisCO catalytic traits and the chemical mechanism of RuBisCO carboxylation and oxygenation reactions raises the possibility of finding unrevealed landscapes in RuBisCO evolution.
Evidence of sedimentation inequality along riparian areas colonised by Impatiens glandulifera (Himalayan balsam)
Authors: Greenwood P , Gange A C , Kuhn N J 
Published: December 2019

SummarySoil loss from riparian areas supporting the annual invasive weed, Impatiens glandulifera (Himalayan balsam), was measured and compared with equivalent values recorded at nearby, topographically similar areas supporting perennial vegetation over a cumulative seven‐year period, along sections of two separate river systems; one in Switzerland, and one in the UK. Soil loss from colonised locations was significantly greater than from reference locations in four of the seven measurement periods. Despite contrasting results, standard deviations, based on soil losses and gains, were predominantly higher for colonised areas at both rivers over most monitoring periods. These findings indicated that areas colonised by Himalayan balsam experience higher sediment flux in comparison with areas free of invasion. Here, we test those original interpretations by reinterrogating the datasets using a more robust analysis of inequality. Nine datasets were tested, five of which (i.e. 56%) showed that sediment flux was significantly greater at Himalayan balsam‐invaded areas than at reference areas. Three datasets showed no difference in sediment flux between invaded and reference areas (33%), and one (11%) showed higher sediment flux at reference areas. Most results uphold our original interpretations and support our hypothesis that hydrochory probably dictates where colonisation initially occurs, by depositing Himalayan balsam seeds in slack or depressional areas along river margins. Once Himalayan balsam becomes established and sufficient perennial vegetation is displaced, seasonal die‐off and depleted vegetation cover may increase the risk that some areas will experience significantly higher sediment flux.
Reviewer Acknowledgements
Authors: Oscar William 
Published: December 2019

International Journal of Contemporary Education (IJCE) would like to acknowledge the following reviewers for their assistance with peer review of manuscripts for this issue. Many authors, regardless of whether IJCE publishes their work, appreciate the helpful feedback provided by the reviewers. Their comments and suggestions were of great help to the authors in improving the quality of their papers. Each of the reviewers listed below returned at least one review for this issue.Reviewers for Volume 2, Number 2Alexandra Ingram, University of Tennessee, USAÁlvaro Manzano Redondo, UCJC University, SpainAurora Q. Pestano, University of San Jose Recoletos, PhilippinesBlessing Dwumah Manu, Jiangsu University, GhanaDina Radeljas, Mohawk Valley Community College, USAFederica Cornali, University of Turin, ItalyFroilan Delute Mobo, Philippine Merchant Marine Academy, PhilippinesGiuseppe Maugeri, Ca' Foscari University, ItalyInaad M Sayer, University of Human Development, IraqIonel Bondoc, University of Agricultural Sciences and Veterinary Medicine Iasi, RomaniaIosif Fragkoulis, Hellenic Open University, GreeceJavier Fombona, Univ. Oviedo, SpainLi Li, Bath Spa University, UKMakrina Nina Zafiri, Aristotle University of Thessaloniki, GreeceMatthew Schatt, University of Florida, USAMs. Bruna Gabriela Augusto Marçal Vieira, Universidade Estadual Paulista, BrazilMurat Tezer, Near East University, CyprusNesrin Ozturk, Ege University, TurkeyNoelia Navarro Gómez, Universidad de Almería, SpainRaymond Aaron Younis, ACU Australia, AustraliaSaid K. Juma, State University of Zanzibar, TanzaniaSandro Sehic, Oneida BOCES, USASuriadi Samsuri, Institute of Islamic Religion Sultan Muhammad Syafiuddin Sambas, IndonesiaTeresa Pozo-Rico, University of Alicante, SpainVassiliki Pliogou, Metropolitan College of Thessaloniki, GreeceVassilios Papadimitriou, University of Thessaly, GreeceXiaojing Sun, Utrecht University, The Netherlands William OscarEditorial AssistantInternational Journal of Contemporary Education---------------------------------------------------------Redfame Publishing9450 SW Gemini Dr. #99416Beaverton, OR 97008, USATel: 1-503-828-0536 ext. 509Fax: 1-503-828-0537E-mail: ijce@redfame.comURL: http://ijce.redfame.com