bluuebunny/crossref_metadata_2025_split huggingface dataset:
Insights on bio-degumming of kenaf bast based on metagenomic and proteomics
Authors: Duan Shengwen , Cheng Lifeng , Feng Xiangyuan , Yang Qi , Liu Zhiyuan , Zheng Ke , Peng Yuande 
Published: December 2019

Abstract
Background: Microbial play important roles in kanef-degumming. This study aims at identifying the key candidate microbial and proteins responsible for the degumming of kenaf bast ( Hibiscus cannabinus ). Kenaf bast was cut into pieces and immersed into microbial fermentation liquid collected from different sites. Fermentation liquid samples were collected at 0, 40, 110 and 150 h and then subjected to the 16S/18S rRNA sequencing analysis and isobaric tag for relative and absolute quantitation (iTRAQ) analysis. The microbial (bacterial and fungal) diversity and the differentially expressed proteins/peptides (DEPs) were identified. Results: With the prolonged degumming time, the weight loss rate increased, the bacterial diversity was decreased. [Weeksellaceae], Enterobacteriaceae and Moraxellaceae were rapidly increased at 0~40 h, and then decreased and were gradually replaced by Bacteroidaceae from 40 h to 150 h. Similarly, Chryseobacterium and Dysgonomonas were gradually increased at 0~110 h and then decreased; Acinetobacter and Lactococcus were increased at 0~40 h, followed by decrease. Bacteroides was the dominant genus at 150 h. 18S rRNA-seq showed the gradually decreased Wallemia_hederae and increased Codosiga_hollandica during degumming. iTRAQ data analysis showed Rds1, and pyruvate kinase I was decreased and increased in the kanef-degumming, respectively. Other DEPs of ferredoxin I, superoxide dismutase and aconitatehydratase were identified to be related to the Glyoxylate and dicarboxylate metabolism (ko00630). Conclusions : Bacteria including Chryseobacterium , Dysgonomonas , Acinetobacter , Lactococcus and Bacteroides and fungi like Wallemia_hederae and Codosiga_hollandica are key candidate microbial for kanef degumming.
Effect of pregnancy induced hypertension on adverse perinatal outcomes in Tigray regional state, Ethiopia: a prospective cohort study
Authors: Berhe Abadi Kidanemariam , ILESANMI Abiodun O. , AIMAKHU Christopher O. , Mulugeta Afework 
Published: December 2019

Abstract
Background: The prevalence of pregnancy-induced hypertension in Ethiopia ranges from 2.2 to 18.3%. However, so far little is known about the adverse perinatal outcomes of pregnancy-induced hypertension in Tigray regional state, Ethiopia. Therefore, the objective of this study was to assess the effect of pregnancy-induced hypertension on adverse perinatal outcomes in Tigray Regional State, Ethiopia. Methods: a prospective cohort study was conducted on a total sample of 782 pregnant women attending antenatal care in hospitals of Tigray regional state, Ethiopia. Pregnant mothers diagnosed with PIH during the data collection period in the selected hospitals were included as exposed group and normotensive women were also enrolled as a control group. Data were collected using an interviewer-administered questionnaire and review of their medical records from February 2018, to February 2019. The adverse perinatal outcome event includes low birth weight, birth asphyxia, small for gestational age, preterm delivery, admission to neonatal intensive care unit and perinatal death. A modified Poisson regression model with robust standard errors was used to analyze relative risk. Results: In this study, the overall incidence of adverse perinatal outcome was higher among women with pregnancy-induced hypertension than normotensive women (66.4% vs 22.2%). After adjusted for confounders women with pregnancy-induced hypertension were born babies with a higher risk of low birth weight (adjusted RR(95%CI)= 5.1(3.4,7.8)), birth asphyxia (aRR=2.6(1.9,3.8)), small for gestational age (aRR=3.3(2.3,4.6)), preterm delivery (aRR=5.2(3.4,7.9)), stillbirth (aRR=3.46(1.40,8.54)), admission to neonatal intensive care unit (aRR=5.1(3.1,8.4)) and perinatal death (aRR=3.6(1.8,7.4)) compared to normotensive pregnant women. Conclusions: Higher incidences of adverse perinatal outcomes occurred among women pregnancy-induced hypertension in Tigray regional state, Ethiopia. Hence health care providers should strengthen prevention, early diagnosis and prompt management of pregnancy-induced hypertension to reduce adverse perinatal outcomes of pregnancy-induced hypertension. Keywords: Adverse perinatal outcomes, pregnancy-induced hypertension, Tigray, Ethiopia
Gene expression identifies metabolic and functional differences between intramuscular and subcutaneous adipocytes in cattle
Authors: Hudson Nicholas James , Reverter Antonio , Griffiths William J. , Yutuc Eylan , Wang Yuqin , Jeanes Angela , McWilliam Sean , Pethick David W. , Greenwood Paul L. 
Published: December 2019

Abstract
Background This study used a genome-wide screen of gene expression to better understand the metabolic and functional differences between commercially valuable intramuscular fat (IMF) and commercially wasteful subcutaneous (SC) fat depots in Bos taurus beef cattle.Results We confirmed many findings previously made at the biochemical level and made new discoveries. The fundamental lipogenic machinery, such as ACACA and FASN encoding the rate limiting Acetyl CoA carboxylase and Fatty Acid synthase were expressed at 1.6-1.8 fold lower levels in IMF, consistent with previous findings. The FA elongation pathway including the rate limiting ELOVL6 was also coordinately downregulated in IMF compared to SC as expected. A 2-fold lower expression in IMF of ACSS2 encoding Acetyl Coenzyme A synthetase is consistent with utilisation of less acetate for lipogenesis in IMF compared to SC as previously determined using radioisotope incorporation. Reduced saturation of fat in the SC depot is reflected by 2.4 fold higher expression of the SCD gene encoding the Δ9 desaturase enzyme. Surprisingly, CH25H encoding the cholesterol 25 hydroxylase enzyme was ~36 fold upregulated in IMF compared to SC. Moreover, its expression in whole muscle tissue appears representative of the proportional representation of bovine marbling adipocytes. This suite of observations prompted quantification of a set of oxysterols (oxidised forms of cholesterol) in the plasma of 8 cattle exhibiting varying IMF. Using LC-MS we found the levels of several oxysterols were significantly associated with multiple marbling measurements across the musculature, but (with just one exception) no other carcass phenotypes.Conclusions These data build on our molecular understanding of ruminant fat depot biology and suggest oxysterols represent a promising circulating biomarker for cattle marbling.
Impact of Lesinurad and Allopurinol on Experimental Hyperuricemia in mice: Biochemical, Molecular and Immunohistochemical Study
Authors: Alghamdi Youseef , Soliman Mohamed Mohamed , Nasan Mohamed 
Published: December 2019

AbstractBackground : Hyperuricemia is an abnormal increase in uric acid levels in the blood. It is the cause of gout that manifested by inflammatory arthritis and painful disable. Therefore, current study evaluated the potential ameliorative impact of Lesinurad and Allopurinol on the kidneys of hyperuricemic mice at the biochemical, molecular and cellular levels. Methods : Lesinurad and allopurinol alone or in combination were orally administered to hyperuricemic and control mice for seven consecutive days. Levels of uric acid and blood urea nitrogen, along with antioxidants and inflammatory cytokines (IL-1β and TNF-a) were measured in the serum. The mRNA expression of mouse urate anion transporter-1, glucose transporter 9, organic anion transporters, in renal tissues were examined using quantitative real time PCR (qRT-PCR). Simultaneously, the immunoreactivity of transforming growth factor-beta 1 was examined immunohistochemically. Results : Lesinurad and allopurinol administration resulted in significant decrease in serum levels of uric acid, blood urea nitrogen, xanthine oxidase activity, catalase, glutathione peroxidase and inflammatory cytokines (IL-1β and TNF-a) reported in hyperuricemic mice. Both partially reversed oxonate-induced alterations in renal mURAT-1, mGLUT-9, mOAT-1 and mOAT-3 expressions, as well as alterations in the immunoreactivity of TGF- β1, resulting in the increase of renal uric acid secretion and excretion. The combined administration of lesinurad and ALP restored all altered parameters in a synergistic manner, improving renal function in the hyperuricemic mouse model employed. Conclusion : This study confirmed synergistic ameliorative hypouricemic impact of both lesinurad and allopurinol in the treatment of hyperuricemia in mice at the biochemical, molecular and cellular levels.
Pregnant women with gestational diabetes mellitus exhibit unique features in oral microbiome
Authors: Li Xiaoqing , Zheng Jiayong , Ma Xiuling , Zhang Bing , Zhang Jinyang , Wang Wenhuan , Sun Congcong , Wang Yeping , Zheng Jianqiong , Chen Haiying , Tao Jiejing , Wang Hai , Zhang Fengyi , Wang Jinfeng , Zhang Hongping 
Published: December 2019

AbstractBackground: Gestational diabetes mellitus (GDM) leads to a series of adverse pregnancy outcomes, seriously endangering the health of mothers and infants. Oral glucose tolerance test (OGTT) is the gold standard test for GDM diagnosis, but largely increases the discomfort of pregnant women, and is inconvenient to follow-up and detection. Considering that oral sampling is convenient, rapid, safe and non-invasive, the feasibility of distinguishing GDM via biomarkers from oral microbiota was evaluated in this study. Here, the 16S rRNA gene sequencing was used to compare the microbiome of saliva and dental plaque samples of 111 pregnant women, and to analyze the structure of oral microbiota in patients with GDM as well as to find effective biomarkers. Results: The results showed that the microbiota of both types of oral samples in patients with GDM changed, and it was quite different from that of neither periodontitis nor dental caries. By using bacterial biomarkers from oral microbiota, GDM classification models based on SVM and random forest algorithms were constructed. For the SVM algorithm, the AUC value of the classification model constructed by the combination of dental plaque Lautropia , Neisseria and saliva Veillonella achieved 0.83. For the random forest algorithm, the maximum AUC value of the model constructed by the dental plaque Streptococcus , Eikenella , Anoxybacillus and saliva Leptotrichia , Kingella was larger than 0.90. Conclusions: These findings revealed that certain bacteria of either saliva or dental plaque can accurately distinguish GDM from healthy pregnant women, which provides a potential non-invasive approach for GDM diagnosis with oral microbial markers.
The Effect of Intermittent Kangaroo Mother Care on Breastfeeding Practice of Late Preterm Infants in China
Authors: Zhang Bo , Duan Zhiying , Zhao Yingxi , Williams Sarah , Wall Stephen , Huang Limin , Zhang Xiaoqin , Wu Wenli , Yue Jieya , Zhang Lin , Liu Jun , Zhao Gengli 
Published: December 2019

Abstract
Background China has an extremely low exclusive breastfeeding rate. Kangaroo mother care (KMC) has been shown to increase the exclusive breastfeeding rate of babies born extremely or very preterm. However, there is limited evidence of intermittent KMC’s effect on exclusive breastfeeding in late preterm. Using hospital discharge and follow-up data collected from four postnatal wards, we investigated the association between intermittent KMC and breastfeeding practice for late preterm babies in China.Methods Intermittent KMC was recommended to the mothers of all preterm babies admitted between March 2018 and March 2019. Those who agreed to practice KMC were enrolled in the “KMC group”, those who did not in the “no-KMC group”. Basic maternal socio-demographic information was collected, feeding outcome and method were recorded daily whilst in hospital and a follow-up survey of feeding practice was conducted 42 days post-discharge. Calculations for feeding practice were performed separately for both groups. Logistics regression was used to analyze the association between KMC and feeding outcome and method, adjusting for socio-demographic covariates.Results Among the 844 participants in the analysis, 627 (74.3%) chose to perform KMC. More mothers who provided KMC were exclusively breastmilk feeding their newborn in the 24 hours before discharge (54.6%) and at follow-up (57.3%), compared with mothers who did not provide KMC (34.6% at discharge and 33.2% at follow-up,). Mothers in the KMC group were more likely to be breastfeeding (method) than mothers in the no-KMC group (65.3% vs. 52.1% at discharge, and 83.1% vs. 67.3% at follow up). Logistic regression indicated that compared with the no-KMC group, mothers who provided KMC were twice as likely to be providing their newborns with exclusive breastmilk feeding at discharge (OR=2.15 (1.53-3.02)), use breast feeding method at discharge (OR=1.61 (1.15-2.25)), provide exclusive breastmilk feeding at follow-up (OR=2.55 (1.81-3.61)), and use breast feeding method at follow-up (OR=2.09 (1.44-3.02)).Conclusions Intermittent KMC was associated with a nearly doubled increase in exclusive breastmilk feeding (outcome) and breast feeding (method) at both discharge and 42 days after discharge for late preterm babies. Intermittent KMC has the potential to increase exclusive breastfeeding rates amongst late preterm infants.
Leaving no one behind: lessons from implementation of policies for universal HIV treatment to universal health coverage
Authors: Assefa Yibeltal , Hill Peter S , Damme Wim Van , Dean Judith , Gilks Charles F 
Published: December 2019

Abstract
Background The third Sustainable Development Goal (SDG-3) aims to ensure healthy lives and promote well-being for all at all ages. SDG-3 has a specific target on universal health coverage (UHC), which emphasizes the importance of all people and communities having access to quality health services without risking financial hardship. The objective of this study is to review progress towards UHC using antiretroviral treatment (ART) as a case study.Methods We used a mixed-methods design including qualitative and quantitative approaches. We reviewed and synthesised the evidence on the evolution of the WHO HIV treatment guidelines between 2002 and 2019. We calculated ART coverage over time by gender, age group, and location. We also estimated ART coverage differences and ratios.Findings ART guidelines have evolved from “treating the sickest” to “treating all”. ART coverage increased globally from under 7% in 2005 to 62% in 2018. There have been successes in increasing ART coverage in all populations and locations. However, progress varies by population and location in many regions. There is inequity in ART coverage: women (68%) versus men (55%), and adults (62%) versus children (54%). This inequity has widened over time, and with expanded ART eligibility criteria. On the other hand, data from at least one high-burden country (Ethiopia) shows that inequity among regions has narrowed over time due to the improvements in the primary health care systems and implementation of the public health approach in the country.Conclusion ART coverage has increased at global, regional and national levels to all population groups. However, the gains have not been equitable among locations and populations. Policies towards universality may widen the inequity in resource-limited settings. We argue that primary health care and public health approaches, with multi-sectoral actions, are vital to minimize inequity and achieve UHC.
Comparison of Methods for Handling Covariate Missingness in Propensity Score Estimation with a Binary Exposure
Authors: Coffman Donna , Zhou Jiangxiu , Cai Xizhen 
Published: December 2019

Abstract
Background Causal effect estimation with observational data is subject to bias due to confounding, which is often controlled for using propensity scores. One unresolved issue in propensity score estimation is how to handle missing values in covariates.Method Several approaches have been proposed for handling covariate missingness, including multiple imputation (MI), multiple imputation with missingness pattern (MIMP), and treatment mean imputation. However, there are other potentially useful approaches that have not been evaluated, including single imputation (SI) + prediction error (PE), SI+PE + parameter uncertainty (PU), and Generalized Boosted Modeling (GBM), which is a nonparametric approach for estimating propensity scores in which missing values are automatically handled in the estimation using a surrogate split method. To evaluate the performance of these approaches, a simulation study was conducted.Results Results suggested that SI+PE, SI+PE+PU, MI, and MIMP perform almost equally well and better than treatment mean imputation and GBM in terms of bias; however, MI and MIMP account for the additional uncertainty of imputing the missingness.Conclusions Applying GBM to the incomplete data and relying on the surrogate split approach resulted in substantial bias. Imputation prior to implementing GBM is recommended.
Technology as the Key to Women’s Empowerment: A Scoping Review
Authors: Mackey April , Petrucka Pammla 
Published: December 2019

Abstract
Background: The achievement of gender equity and equality has been a long-time goal of many international entities . The main indicator for the goal of women’s empowerment, as part of the Sustainable Development Goals (SDGs), has been to enable the use of technology by increasing the current access for women and girls across the globe. While information and communication technologies (ICTs) were initially thought to be neutral in terms of access and opportunity, emerging trends now indicate that the use of technology within society has significant social implications, specifically related to gender as a determinant of health. Methods : Using the Arksey and O’Malley methodology for scoping reviews, the following question was answered: What is the impact of information and communication technology (ICT) on the level of women’s empowerment worldwide? The primary objective of this scoping review was to identify the extent, range, and scope of evidence involving the impact and influence of ICTs on women’s empowerment. A total of 51 articles were analyzed using the scoping review framework. Results: The major themes that emerged from this review included: (a) the means in which ICTs have assisted in building the capacity and tools of women, (b) the manner in which ICTs have been used as an intervention in supporting empowerment; and (c) the approach in which ICTs can act as potential barriers and facilitators to women’s attainment of agency. Conclusions: The evidence from this scoping review supports the innovative use of current and emerging technologies within health care to connect with, engage, and empower women both within the acute and community settings. The extant evidence explores how ICT has played a role in the promotion and support of women ’s empowerment as well as supporting the development of health care policies and relevant programs.
On Sackin's original proposal: The variance of the leaves' depths as a phylogenetic balance index
Authors: Coronado Tomás Martínez , Mir Arnau , Rossello Francesc , Rotger Lucía 
Published: December 2019

Abstract
Background: The Sackin index S of a rooted phylogenetic tree, defined as the sum of its leaves' depths, is one of the most popular balance indices in phylogenetics, and Sackin's 1972 paper is usually cited as the source for this index. However, what Sackin actually proposed in his paper as a measure of the imbalance of a rooted tree was not the sum of its leaves' depths, but their "variation". This proposal was later implemented as the variance of the leaves' depths by Kirkpatrick and Slatkin, where moreover they posed the problem of finding a closed formula for its expected value under the Yule model. Nowadays, Sackin's original proposal seems to have passed into oblivion in the phylogenetics literature, replaced by the index bearing his name, which, in fact, was introduced a decade later by Sokal.Results: In this paper we study the properties of the variance of the leaves' depths, V, as a balance index. Firstly, we prove that the rooted trees with n leaves and maximum V value are exactly the combs with n leaves. But although V achieves its minimum value on every space BT_n of bifurcating rooted phylogenetic trees with n< 184 leaves at the so-called "maximally balanced trees" with n leaves, this property fails for almost every n>= 184. We provide then an algorithm that finds in O(n) time the trees in BT_n with minimum V value. Secondly, we obtain closed formulas for the expected V value of a bifurcating rooted tree with any number n of leaves under the Yule and the uniform models and, as a by-product of the computations leading to these formulas, we also obtain closed formulas for the variance of the Sackin index and the total cophenetic indexof a bifurcating rooted tree, as well as of their covariance, under the uniform model, thus filling this gap in the literature.Conclusions: The phylogenetics crowd has been wise in preferring as a balance index the sum S(T) of the leaves’ depths of a phylogenetic tree T over their variance V (T), because the latter does not seem to capture correctly the notion of balance of large bifurcating rooted trees. But for bifurcating trees up to 183 leaves, V is a valid and useful balance index.
Antibiotic treatment for bone infection after debridement: 2 weeks or 6 weeks?
Authors: Wang Xiaohua , Fang Li , Wang Shulin , Ma Huan , Zhao Hongwen , Xie Zhao 
Published: December 2019

Abstract
Purpose: Our aim was to investigate the clinical efficacy and complications of antibiotics treatment duration for the patients of bone infection. Methods: We retrospectively analyzed the patients with bone infection admitted to our hospital between March 2013 and October 2018. The surgical debridement was performed and the patients were divided into three groups: IV group (Intravenous antibiotics for 2 weeks); Oral group (Intravenous antibiotics for 2 weeks followed by oral antibiotics for 4 weeks); Rifampicin group (Intravenous antibiotics for 2 weeks followed by oral antibiotics plus rifampicin for 4 weeks). The infection control rate and complications were compared. Results ： A total of 902 patients were enrolled, the infection sites included 509 tibias, 228 femurs, 32 humeri, 23 radii and ulnae, 40 calcanei, 23 multiple-site infections and the other sites 47 cases. After at least 6 months of follow-up, 148 (16.4%) patients had recurrence of infection. The recurrence rate of IV group was 17.9%, which was no significant higher than that of Oral group (10.1%) or Rifampicin group (10.5%). The abnormal rate of Glutamic-pyruvic transaminase(ALT) in IV group was 15.1%, which was lower than that of Oral group (18.0%) and Rifampicin group (27.4%), P=0.026. The positive rates of proteinuria in the three groups were 3.2%, 4.5%, and 9.3%, respectively, P=0.020. Conclusion: After debridement of bone infection, the additional oral antibiotic treatment may increase the damage of liver and kidney, and can not significantly reduce the infection recurrence rate. Therefore, it is recommended to adopt short-term systemic antibiotic treatment after debridement.