bluuebunny/crossref_metadata_2025_split huggingface dataset:
Digital contact tracing
Authors: Trivedi Amee , Vasisht Deepak 
Published: October 2020

Since the start of the COVID-19 pandemic, technology enthusiasts have pushed for digital contact tracing as a critical tool for breaking the COVID-19 transmission chains. Motivated by this push, many countries and companies have created apps that enable digital contact tracing with the goal to identify the chain of transmission from an infected individual to others and enable early quarantine. Digital contact tracing applications like AarogyaSetu in India, TraceTogether in Singapore, SwissCovid in Switzerland, and others have been downloaded hundreds of millions of times. Yet, this technology hasn't seen the impact that we envisioned at the start of the pandemic. Some countries have rolled back their apps, while others have seen low adoption [12, 17].
Therefore, it is prudent to ask what the technology landscape of contact-tracing looks like and what are the missing pieces. We attempt to undertake this task in this paper. We present a high-level review of technologies underlying digital contact tracing, a set of metrics that are important while evaluating different contact tracing technologies, and evaluate where the different technologies stand today on this set of metrics. Our hope is two fold: (a) Future designers of contact tracing applications can use this review paper to understand the technology landscape, and (b) Researchers can identify and solve the missing pieces of this puzzle, so that we are ready to face the rest of the COVID-19 pandemic and any future pandemics. A majority of this discussion is focused on the ability to identify contact between individuals. The questions of ethics, privacy, and security of such contact tracing are briefly mentioned but not discussed in detail.
STAT3-Mediated Astrocyte Reactivity Associated with Brain Metastasis Contributes to Neurovascular Dysfunction
Authors: Sarmiento Soto Manuel , Larkin James R. , Martin Chris , Khrapitchev Alexandre A. , Maczka Melissa , Economopoulos Vasiliki , Scott Helen , Escartin Carole , Bonvento Gilles , Serres Sébastien , Sibson Nicola R. 
Published: October 2020

Abstract

Astrocytes are thought to play a pivotal role in coupling neural activity and cerebral blood flow. However, it has been shown that astrocytes undergo morphologic changes in response to brain metastasis, switching to a reactive phenotype, which has the potential to significantly compromise cerebrovascular function and contribute to the neurological sequelae associated with brain metastasis. Given that STAT3 is a key regulator of astrocyte reactivity, we aimed here to determine the impact of STAT3-mediated astrocyte reactivity on neurovascular function in brain metastasis. Rat models of brain metastasis and ciliary neurotrophic factor were used to induce astrocyte reactivity. Multimodal imaging, electrophysiology, and IHC were performed to determine the relationship between reactive astrocytes and changes in the cerebrovascular response to electrical and physiological stimuli. Subsequently, the STAT3 pathway in astrocytes was inhibited with WP1066 to determine the role of STAT3-mediated astrocyte reactivity, specifically, in brain metastasis. Astrocyte reactivity associated with brain metastases impaired cerebrovascular responses to stimuli at both the cellular and functional level and disrupted astrocyte–endothelial interactions in both animal models and human brain metastasis samples. Inhibition of STAT3-mediated astrocyte reactivity in rats with brain metastases restored cerebrovascular function, as shown by in vivo imaging, and limited cerebrovascular changes associated with tumor growth. Together these findings suggest that inhibiting STAT3-mediated astrocyte reactivity may confer significant improvements in neurological outcome for patients with brain metastases and could potentially be tested in other brain tumors.

Significance:
These findings demonstrate that selectively targeting STAT3-mediated astrocyte reactivity ameliorates the cerebrovascular dysfunction associated with brain metastasis, providing a potential therapeutic avenue for improved patient outcome.
RNA sequencing and Immunohistochemistry Reveal ZFN7 as a Stronger Marker of Survival than Molecular Subtypes in G-CIMP–negative Glioblastoma
Authors: Esteve-Codina Anna , Alameda Francesc , Carrato Cristina , Pineda Estela , Arpí Oriol , Martinez-García Maria , Mallo Mar , Gut Marta , Dabad Marc , Tortosa Avelina , Del Barco Sonia , Capellades Jaume , Puig Josep , Gallego Oscar , Pujol Teresa , Oleaga Laura , Gil-Gil Miquel , de Quintana-Schmidt Cristian , Valduvieco Izaskun , Martinez-Cardús Anna , Bellosillo Beatriz , Muñoz-Marmol Ana Maria , Esteve Anna , Domenech Marta , Camins Angels , Craven-Bartle Jordi , Villa Salvador , Marruecos Jordi , Domenech Sira , de la Iglesia Nuria , Balana Carmen 
Published: October 2020

Abstract

Purpose:
Glioblastoma is the most aggressive brain tumor in adults and has few therapeutic options. The study of molecular subtype classifications may lead to improved prognostic classification and identification of new therapeutic targets. The Cancer Genome Atlas (TCGA) subtype classification has mainly been applied in U.S. clinical trials, while the intrinsic glioma subtype (IGS) has mainly been applied in European trials.

Experimental Design:
From paraffin-embedded tumor samples of 432 patients with uniformly treated, newly diagnosed glioblastoma, we built tissue microarrays for IHC analysis and applied RNA sequencing to the best samples to classify them according to TCGA and IGS subtypes.

Results:
We obtained transcriptomic results from 124 patients. There was a lack of agreement among the three TCGA classificatory algorithms employed, which was not solely attributable to intratumoral heterogeneity. There was overlapping of TCGA mesenchymal subtype with IGS cluster 23 and of TCGA classical subtype with IGS cluster 18. Molecular subtypes were not associated with prognosis, but levels of expression of 13 novel genes were identified as independent prognostic markers in glioma-CpG island methylator phenotype–negative patients, independently of clinical factors and MGMT methylation. These findings were validated in at least one external database. Three of the 13 genes were selected for IHC validation. In particular, high ZNF7 RNA expression and low ZNF7 protein expression were strongly associated with longer survival, independently of molecular subtypes.

Conclusions:
TCGA and IGS molecular classifications of glioblastoma have no higher prognostic value than individual genes and should be refined before being applied to clinical trials.
Cellular Functions of HPV16 E5 Oncoprotein during Oncogenic Transformation
Authors: Gutierrez-Xicotencatl Lourdes , Pedroza-Saavedra Adolfo , Chihu-Amparan Lilia , Salazar-Piña Azucena , Maldonado-Gama Minerva , Esquivel-Guadarrama Fernando 
Published: October 2020

AbstractThe human papillomavirus (HPV) is recognized as the main etiologic agent associated with cervical cancer. HPVs are epitheliotropic, and the ones that infect the mucous membranes are classified into low-risk (LR) and high-risk (HR) types. LR-HPVs produce benign lesions, whereas HR-HPVs produce lesions that may progress to cancer. HR-HPV types 16 and 18 are the most frequently found in cervical cancer worldwide. E6 and E7 are the major HPV oncogenic proteins, and they have been profusely studied. Moreover, it has been shown that the HPV16 E5 (16E5) oncoprotein generates transformation, although the molecular mechanisms through which it carries out its activity have not been well defined. In contrast to E6 and E7, the E5 open reading frame is lost during the integration of the episomal HPV DNA into the cellular genome. This suggests that E5 acts at the early stages of the transformation process. In this review, we focused on the biochemical characteristics and functions of the HPV E5 oncoprotein, mainly on its association with growth factor receptors and other cellular proteins. Knowledge of the HPV E5 biology is important to understand the role of this oncoprotein in maintaining the viral cycle through the modulation of proliferation, differentiation, and apoptosis, as well as the alteration of other processes, such as survival, adhesion, migration, and invasion during early carcinogenesis. Finally, we summarized recent research that uses the E5 oncoprotein as a therapeutic target, promising a novel approach to the treatment of cervical cancer in its early stages.
Avifaunal diversity along the riverine habitats of Papikonda National Park, Andhra Pradesh, India
Authors: Ray Paromita , Malla Giridhar , Manral Upma , J.A. Johnson  , K. Sivakumar  
Published: October 2020

This study was carried out to record the avifaunal diversity of the riverine habitats along the Godavari River in Papikonda National Park, during a short survey conducted from 2017 to 2018. A total of 63 bird species belonging to 25 families were recorded during the survey. The study resulted in the recording of eight globally threatened avian species including the Endangered Black-bellied Tern Sterna acuticauda, and seven Near Threatened species, viz.: Painted Stork Mycteria leucocephala, Black-headed Ibis Threskiornis melanocephalus, Great Thick Knee Esacus recurvirostris, River Lapwing Vanellus duvaucelli, Malabar Pied Hornbill Anthracoceros coronatus, Grey-headed Fish Eagle Haliaeetus ichthyaetus, and Oriental Darter Anhinga melanogaster. Species including Black-bellied Tern Sterna acuticauda, Grey-headed Fish Eagle Haliaeetus ichthyaetus, Indian Eagle-owl Bubo bengalensis, and Black Eagle Ictinaetus malaiensis were recorded for the first time along the riverine habitats of Papikonda National Park. Sand mining of the riverbed and discharge of effluents into the Godavari River has already negatively impacted the associated habitats and avifaunal diversity.
A highway to hell: a proposed, inessential, 6-lane highway (NH173) that threatens the forest and wildlife corridors of the Western Ghats, India
Authors: Sagar H.S. Sathya Chandra , Mrunmayee  
Published: October 2020

A globally, extensive road network combined with increasing vehicular traffic poses a significant threat to local wildlife, environment, economy, and socio-politics. India, with nearly 5.9 million kilometers of road, has the second-highest road network in the world; and has plans to exponentially increase its national highways. In this study, we use a combination of collation of official documents, literature review, and GIS mapping to outline the possible environmental and socio-economic impacts caused by a proposed 6-lane national highway (NH 173). This highway is set to cut through the low elevation evergreen forests of the central Western Ghats between Mudigere and Nelliyadi towns of Chikkamagaluru and Dakshina Kannada districts, of Karnataka State, respectively. We further outline the insignificance of the project and recommend workable alternatives that could be considered in the wider public’s interest.
Cirsium wallichii DC. (Asteraceae): a key nectar source of butterflies
Authors: Boruah Bitupan , Kumar Amit , Das Abhijit 
Published: October 2020

Abstract: Butterfly diversity and abundance depend on the diversity of foraging resources and nectar characteristics. Several wild plants of the family Asteraceae often considered as weeds, are important nectar sources for butterflies. The present communication reports Cirsium wallichii as a key nectar source for a large number of butterfly species during the summer season in Benog Wildlife Sanctuary, Mussoorie, western Himalaya. Of the total recorded species of butterflies (62), a maximum species (22) belongs to the Nymphalidae family followed by Hesperiidae (13), and Lycaenidae (11) that visits C. wallichii. Therefore, it is suggested that Cirsium wallichii has an important role in sustaining butterfly diversity.

Keywords: Mussoorie, Lepidoptera, .
Medetomidine may cause heart murmur in Cougars and Jaguars: case report
Authors: Thiago Cavalheri Luczinski  , Gediendson Ribeiro de Araújo  , Matheus Folgearini Silveira  , Murillo Daparé Kirnew  , Navarrete Roberto Andres , Salomão-Jr Jorge Aparecido , Letícia Alecho Requena  , Jairo Antonio Melo dos Santos  , Marcell Hideki Koshiyama  , Cristiane Schilbach Pizzutto  , Jorge-Neto Pedro Nacib 
Published: October 2020

We report heart murmur in Jaguars and Cougars found during reproductive procedures for semen and oocyte collection. Two male Cougars (n=2) and three female Jaguars (n=3) were examined. Anesthesia was performed with ketamine and medetomidine in males. Females also received propofol and were maintained with isoflurane. The animals were evaluated during anesthetic monitoring with multiparameter monitor alongside clinical examination, ambulatory electrocardiogram and echocardiogram. All animals presented mitral valve regurgitation under anesthesia, but without morphological changes in the cardiac structure or hemodynamic changes. Medetomidine may cause transitory heart murmur in healthy Jaguars and Cougars.
Association of Exhaled Carbon Monoxide With Ideal Cardiovascular Health, Circulating Biomarkers, and Incidence of Heart Failure in the Framingham Offspring Study
Authors: Tun Bradley , Ehrbar Rachel , Short Meghan , Cheng Susan , Vasan Ramachandran S. , Xanthakis Vanessa 
Published: October 2020

Background
Exhaled carbon monoxide (eCO) is directly associated with traditional cardiovascular disease risk factors and incident cardiovascular disease. However, its relation with the cardiovascular health score and incidence of heart failure (HF) has not been investigated.

Methods and Results

We measured eCO in 3521 Framingham Heart Study Offspring participants attending examination cycle 6 (mean age 59 years, 53% women). We related the cardiovascular health score (composite of blood pressure, fasting plasma glucose, total cholesterol, body mass index, smoking, diet, and physical activity) to eCO adjusting for age, sex, and smoking. Higher cardiovascular health scores were associated with lower eCO (β=−0.02,
P
<0.0001), even among nonsmokers. Additionally, C‐reactive protein, plasminogen activator inhibitor‐1, fibrinogen, growth differentiation factor‐15, homocysteine, and asymmetrical dimethylarginine were positively associated with eCO (
P
≤0.003 for all). The age‐ and sex‐adjusted and multivariable‐adjusted heritabilities of eCO were 49.5% and 31.4%, respectively. Over a median follow‐up of 18 years, 309 participants (45% women) developed HF. After multivariable adjustment, higher eCO was associated with higher risk of HF (hazards ratio per SD increment: 1.39; 95% CI, 1.19–1.62 [
P
<0.001]) and with higher risk of HF with reduced ejection fraction (N=144 events; hazard ratio per SD increment in eCO: 1.43; 95% CI, 1.15–1.77 [
P
=0.001]).

Conclusions
In our community‐based sample, higher levels of eCO were associated with lower cardiovascular health scores, an adverse cardiovascular biomarker profile, and a higher risk of HF, specifically HF with reduced ejection fraction. Our findings suggest that carbon monoxide may identify a novel pathway to HF development.
MetOp/AVHRR Snow Detection Method for Meteorological Applications
Authors: Siljamo Niilo , Hyvärinen Otto , Riihelä Aku , Suomalainen Markku 
Published: October 2020

AbstractSnow cover plays a significant role in the weather and climate system by affecting the energy and mass transfer between the surface and the atmosphere. It also has far-reaching effects on ecosystems of snow-covered areas. Therefore, global snow-cover observations in a timely manner are needed. Satellite-based instruments can be utilized to produce snow-cover information that is suitable for these needs. Highly variable surface and snow-cover features suggest that operational snow extent algorithms may benefit from at least a partly empirical approach that is based on carefully analyzed training data. Here, a new two-phase snow-cover algorithm utilizing data from the Advanced Very High Resolution Radiometer (AVHRR) on board the MetOp satellites of the European Organization for the Exploitation of Meteorological Satellites (EUMETSAT) is introduced and evaluated. This algorithm is used to produce the MetOp/AVHRR H32 snow extent product for the Satellite Application Facility on Support to Operational Hydrology and Water Management (H SAF). The algorithm aims at direct detection of snow-covered and snow-free pixels without preceding cloud masking. Pixels that cannot be classified reliably to snow or snow-free, because of clouds or other reasons, are set as unclassified. This reduces the coverage but increases the accuracy of the algorithm. More than four years of snow-depth and state-of-the-ground observations from weather stations were used to validate the product. Validation results show that the algorithm produces high-quality snow coverage data that may be suitable for numerical weather prediction, hydrological modeling, and other applications.
The Detailed Dynamics of the Hadley Cell. Part II: December–February
Authors: Hoskins B. J. , Yang G.-Y. 
Published: October 2020

AbstractThis paper complements an earlier paper on the June–August Hadley cell by giving a detailed analysis of the December–February Hadley cell as seen in a 30-yr climatology of ERA-Interim data. The focus is on the dynamics of the upper branch of the Hadley cell. There are significant differences between the Hadley cells in the two solsticial seasons. These are particularly associated with the ITCZs staying north of the equator and with mean westerlies in the equatorial regions of the east Pacific and Atlantic in December–February. The latter enables westward-moving mixed Rossby–gravity waves to be slow moving in those regions and therefore respond strongly to upstream off-equatorial active convection. However, the main result is that in both seasons it is the regions and times of active convection that predominantly lead to upper-tropospheric outflows and structures that average to give the mean flow toward the winter pole, and the steady and transient fluxes of momentum and vorticity that balance the Coriolis terms. The response to active convection in preferred regions is shown by means of regressions on the data from the climatology and by synoptic examples from one season. Eddies with tropical origin are seen to be important in their own right and also in their interaction with higher-latitude systems. There is support for the relevance of a new conceptual model of the Hadley cell based on the sporadic nature of active tropical convection in time and space.
The Rapid Intensification of Hurricane Michael (2018): Storm Structure and the Relationship to Environmental and Air–Sea Interactions
Authors: Wadler Joshua B. , Zhang Jun A. , Rogers Robert F. , Jaimes Benjamin , Shay Lynn K. 
Published: October 2020

AbstractThe spatial and temporal variation in multiscale structures during the rapid intensification of Hurricane Michael (2018) are explored using a coupled atmospheric–oceanic dataset obtained from NOAA WP-3D and G-IV aircraft missions. During Michael’s early life cycle, the importance of ocean structure is studied to explore how the storm intensified despite experiencing moderate vertical shear. Michael maintained a fairly symmetric precipitation distribution and resisted lateral mixing of dry environmental air into the circulation upshear. The storm also interacted with an oceanic eddy field leading to cross-storm sea surface temperature (SST) gradients of ~2.5°C. This led to the highest enthalpy fluxes occurring left of shear, favoring the sustainment of updrafts into the upshear quadrants and a quick recovery from low-entropy downdraft air. Later in the life cycle, Michael interacted with more uniform and higher SSTs that were greater than 28°C, while vertical shear imposed asymmetries in Michael’s secondary circulation and distribution of entropy. Midlevel (~4–8 km) outflow downshear, a feature characteristic of hurricanes in shear, transported high-entropy air from the eyewall region outward. This outflow created a cap that reduced entrainment across the boundary layer top, protecting it from dry midtropospheric air out to large radii (i.e., >100 km), and allowing for rapid energy increases from air–sea enthalpy fluxes. Upshear, low-level (~0.5–2 km) outflow transported high-entropy air outward, which aided boundary layer recovery from low-entropy downdraft air. This study underscores the importance of simultaneously measuring atmospheric and oceanographic parameters to understand tropical cyclone structure during rapid intensification.
Clinical effectiveness of peroneal nerve functional electrical stimulation in chronic stroke patients with hemiplegia (PLEASURE): A multicentre, prospective, randomised controlled trial
Authors: Hachisuka Kenji , Ochi Mitsuhiro , Kikuchi Takashi , Saeki Satoru 
Published: October 2020

Objective: To compare the effectiveness of gait training using a peroneal nerve stimulation device with the effectiveness of gait training without the device in improving gait ability and ankle-specific body functions. Design: Multicentre, prospective, randomised, open-label trial. Setting: Twenty-three hospitals. Subjects: In total, 119 stroke patients with foot drop were randomly assigned to the experimental (with the device) or control (without the device) group. Intervention: Subjects underwent 480-minute self-directed training over four weeks, followed by 260-minute physical therapist-assisted gait training with or without the device. Main measures: The primary endpoint was a change in the six-minute walk test (6MWT) without the device from baseline to after the four-week intervention. The secondary endpoints were changes in the 10-metre walk test (10MWT) without the device, Fugl-Meyer Assessment, range of motion, muscle strength, Modified Ashworth Scale, Stroke Impact Scale Japanese edition (J-SIS) and adverse events. Results: Fifty-six experimental and 59 control group participants, with an average age of 59 years (SD 12) completed the trial. The 6MWT distance changes (m) for the experimental and control groups were 14.7 (SD 37.6) and 22.2 (SD 49.3), respectively. The 10MWT speed changes (m/sec) for the experimental and control groups were 0.06 (SD 0.12) and 0.07 (SD 0.17), respectively. No significant differences were observed in these changes between both groups, and no differences were found in the other secondary endpoints, except for the J-SIS patient’s subjective assessment ( P = 0.048). Conclusion: The improvement in gait ability and body functions were equivalent with or without the use of the device.
Rare misdiagnosed case of penicilliosis marneffei in an immunocompetent patient
Authors: Cheng Dan , Ding Xuhong 
Published: October 2020

Background Penicilliosis marneffei is a rare disease caused by Talaromyces marneffei, which is endemic in Southeast Asian countries, and usually occurs in immunocompromised or immunodeficient hosts. We report an unusual misdiagnosed case of penicilliosis marneffei in an immunocompetent patient from a non-endemic area of China. Case presentation: A 59-year-old man presented to hospital with a cough and progressive hemoptysis for a 3-month period. Clinical characteristics, radiological abnormalities, and prognosis were analyzed. Detailed examinations, chest computed tomography, and bronchoscopy were performed. The patient was misdiagnosed as having lung cancer and provided anti-tumor treatment for 1 month in the early stage. Imaging and bronchoscopy showed a neoplasm in the basal segment in the left lung, but a tissue biopsy did not establish a diagnosis. He subsequently underwent lobectomy for the lesion. The final pathological diagnosis was penicilliosis marneffei based on immunohistochemical staining. He was then prescribed specific anti-fungal treatment of voriconazole 200 mg twice daily for 12 weeks. Conclusion Timely fungal culture and pathological examination of clinical specimens are critical for the diagnosis of penicilliosis marneffei, especially in non-endemic regions and immunocompetent patients.