bluuebunny/crossref_metadata_2025_split huggingface dataset:
Long-term associations and insights on social structure of the Humpback whales in Prince William Sound, Alaska
Authors: von Ziegesar Olga , Gill Shelley , Goodwin Beth 
Published: March 2020

AbstractHumpback whale (Megaptera novaeangliae) social structure is more complex than previously thought. Because of the fluid “fission-fusion” nature of their relationships: individuals foraging, traveling, and socializing with a number of animals, where associations form and are broken numerous times, little has been confirmed about their long-term associations. Humpback whales of the North Pacific Ocean migrate annually between tropical breeding areas to northern latitudes where they congregate and feed. The purpose of this study was to explore the social and feeding habits of the summer population of humpback whales returning to Prince William Sound (PWS) in the south central coast of Alaska. Fluke photographs of pigmentation patterns were used to document individual whales between the years 1983 and 2009 to determine, population characteristics, reproductive rates, long-term associations, feeding habits and spatial partitioning. During the 27 year study period there were 3,017 encounters with 405 unique whales. Forty of these whales (9.88%) had long sighting histories, showing strong site fidelity. Association indices for all pairs of whales were calculated. Long-lasting associations were found between thirty-two of the forty whales. Two distinct groups were determined by the highest association coefficients. Although the overall ranges of the two groups overlapped, they did not often mingle and offspring did not join their maternal group. All but two females had enduring bonds with at least one male. Associate males were sometimes found at a distance from others of their “clan” and would rejoin periodically. Two whales from one of these clans were found together in Hawaiian waters, a male escorting a female with a newborn calf, suggesting these long lasting associations endure through migration and into the southern breeding areas. Optimal observation conditions of a small population of humpback whales in sheltered waters allowed the discovery of two social groups enduring almost three decades.
A 3K Axiom®SNP array from a transcriptome-wide SNP resource sheds new light on the genetic diversity and structure of the iconic subtropical conifer treeAraucaria angustifolia(Bert.) Kuntze
Authors: Silva Pedro I.T. , Silva-Junior Orzenil Bonfim , Resende Lucileide V. , Sousa Valderes A. , Aguiar Ananda V. , Grattapaglia Dario 
Published: March 2020

AbstractHigh-throughput SNP genotyping has become a precondition to move to higher precision and wider genome coverage genetic analysis of natural and breeding populations of non-model species. We developed a 44,318 annotated SNP catalog forAraucaria angustifolia, a grandiose subtropical conifer tree, one of the only two native Brazilian gymnosperms, critically endangered due to its valuable wood and seeds. Following transcriptome assembly and annotation, SNPs were discovered from RNA-seq and pooled RAD-seq data. From the SNP catalog, an Axiom®SNP array with 3,038 validated SNPs was developed and used to provide a comprehensive look at the genetic diversity and structure of 15 populations across the natural range of the species. RNA-seq was a far superior source of SNPs when compared to RAD-seq in terms of conversion rate to polymorphic markers on the array, likely due to the more efficient complexity reduction of the huge conifer genome. By matching microsatellite and SNP data on the same set ofA. angustifoliaindividuals, we show that SNPs reflect more precisely the actual genome-wide patterns of genetic diversity and structure, challenging previous microsatellite-based assessments. Moreover, SNPs corroborated the known major north-south genetic cline, but allowed a more accurate attribution to regional versus among-population differentiation, indicating the potential to select ancestry-informative markers. The availability of a public, user-friendly 3K SNP array forA. angustifoliaand a catalog of 44,318 SNPs predicted to provide ∼29,000 informative SNPs across ∼20,000 loci across the genome, will allow tackling still unsettled questions on its evolutionary history, toward a more comprehensive picture of the origin, past dynamics and future trend of the species’ genetic resources. Additionally, but not less importantly, the SNP array described, unlocks the potential to adopt genomic prediction methods to accelerate the still very timid efforts of systematic tree breeding ofA. angustifolia.
Citric Acid Water as an Alternative to Water Restriction for High-Yield Mouse Behavior
Authors: Urai Anne E , Aguillon-Rodriguez Valeria , Laranjeira Inês C , Cazettes Fanny ,   The International Brain Laboratory, Mainen Zachary F , Churchland Anne K 
Published: March 2020

AbstractPowerful neural measurement and perturbation tools have positioned mice as an ideal species for probing the neural circuit mechanisms of cognition. Crucial to this success is the ability to motivate animals to perform specific behaviors. One successful strategy is to restrict their water intake, rewarding them with water during a behavioral task. However, water restriction requires rigorous monitoring of animals’ health and hydration status and can be challenging for some mice.We present an alternative that allows mice more control over their water intake: free home-cage access to water, made slightly sour by a small amount of citric acid (CA). In a previous study, rats with free access to CA water readily performed a behavioral task for water rewards, although completing fewer trials than under water restriction (Reinagel, 2018). We here extend this approach to mice and confirm its robustness across multiple laboratories.Mice reduced their intake of CA water while maintaining healthy weights. Continuous home-cage access to CA water only subtly impacted their willingness to perform a decision-making task, in which they were rewarded with sweetened water. When free CA water was used instead of water restriction only on weekends, learning and decision-making behavior were unaffected. CA water is thus a promising alternative to water restriction, allowing animals more control over their water intake without interfering with behavioral performance.SignificanceStatementHigh-throughput, reliable behavioral training is a key requirement for the use of mice in behavioral and systems neuroscience, but depends crucially on ability to motivate animals to perform specific behaviors. Here, we present an alternative method to commonly used methods of water restriction: free home-cage access to water, made slightly sour by a small amount of citric acid. This non-labor-intensive, low-error option benefits animal health without hindering behavioral training progress. Citric acid water can serve as a reliable and standardized strategy to achieve high quality task behavior, further facilitating the use of mice in high-throughput behavioral studies.
Real-time 3D Single Molecule Tracking
Authors: Hou Shangguo , Exell Jack , Welsher Kevin 
Published: March 2020

AbstractTo date, single molecule studies have been reliant on tethering or confinement to achieve long duration and high temporal resolution measurements. Here, we present a 3D single-molecule active real-time tracking method (3D-SMART) which is capable of “locking on” to single fluorophores in solution for minutes at a time with photon limited temporal resolution. As a demonstration, 3D-SMART was applied to actively track single Atto 647N fluorophores in 90% glycerol solution with an average duration of ~16 s at count rates of ~10 kHz. Active feedback tracking was further applied to single proteins and nucleic acids, directly measuring the diffusion coefficient of various lengths (99 to 1385 bp) of single DNA molecules at speeds up to 10 μm2/s. In addition, 3D-SMART was able to quantify to occupancy of single Spinach2 RNA aptamers and capture active transcription on single freely diffusing DNA. 3D-SMART represents a critical step towards the untethering of single molecule spectroscopy.
The K-Cl co-transporter 2 is a point of convergence for multiple autism spectrum disorder and epilepsy risk gene products
Authors: Smalley Joshua L. , Kontou Georgina , Choi Catherine , Ren Qiu , Albrecht David , Abiraman Krithika , Rodriguez Santos Miguel A. , Bope Christopher E. , Deeb Tarek Z. , Davies Paul A. , Brandon Nicholas J. , Moss Stephen J. 
Published: March 2020

ABSTRACTKCC2 plays a critical role in determining the efficacy of synaptic inhibition and deficits in its activity lead to epilepsy and neurodevelopmental delay. Here we use unbiased proteomic analyses to demonstrate that KCC2 forms stable protein complexes in the neuronal plasma membrane with 96 autism and/or epilepsy risk gene (ASD/Epi) products including ANKB, ANKG, CNTN1, ITPR1, NCKAP1, SCN2A, SHANK3, SPTAN1, and SPTBN1. Many of these proteins are also targets of Fragile-X mental retardation protein (FMRP), the inactivation of which is the leading monogenic cause of autism. Accordingly, the expression of a subset of these KCC2-binding partners was decreased in Fmr1 knockout mice. Fmr1 knockout compromised KCC2 phosphorylation, a key regulatory mechanism for transporter activity and the postnatal development of GABAergic inhibition. Thus, KCC2 is a point of convergence for multiple ASD/Epi risk genes and therapies targeting this transporter may have broad utility in alleviating these heterogeneous disorders and their associated epilepsies.
Pannexin 1 regulates spiny protrusion dynamics in cortical neurons
Authors: Sanchez-Arias Juan C. , Candlish Rebecca C. , Swayne Leigh Anne 
Published: March 2020

AbstractThe integration of neurons into networks relies on the formation of dendritic spines. These specialized structures arise from dynamic filopodia-like spiny protrusions. Recently, it was discovered that cortical neurons lacking the channel protein Pannexin 1 (Panx1) exhibited larger and more complicated neuronal networks, as well as, higher dendritic spine densities. Here, we expanded on those findings to investigate whether the increase in dendritic spine density associated with lack of Panx1 was due to differences in the rates of spine dynamics. Using a fluorescent membrane tag (mCherry-CD9-10) to visualize spiny protrusions in developing neurons (at 10days-in-vitro, DIV10) we confirmed that lack of Panx1 leads to higher spiny protrusion density while transient transfection of Panx1 leads to decreased spiny protrusion density. To quantify the impact of Panx1 expression on spiny protrusion formation, elimination, and motility, we used live cell imaging in DIV10 neurons (1 frame every 5 seconds for 10 minutes). We discovered, that at DIV10, lack of Panx1 KO stabilized spiny protrusions. Notably, re-expression of Panx1 in Panx1 knockout neurons resulted in a significant increase in spiny protrusion motility and turnover. In summary, these new data revealed that Panx1 regulates the development of dendritic spines by controlling protrusion dynamics.Significance statementCells in the brain form intricate and specialized networks -neuronal networks- in charge of processing sensations, executing movement commands, and storing memories. To do this, brain cells extend microscopic protrusions -spiny protrusions- which are highly dynamic and survey the local environment to contact other cells. Those contact sites are known as synapses and undergo further stabilization and maturation establishing the function and efficiency of neuronal networks. Our work shows that removal of Panx1 increases the stability and decreases the turnover of spiny protrusion on young neurons.
Accounting to ensure healthy lives: critical perspective from the Italian National Healthcare System
Authors: Pizzi Simone , Caputo Fabio , Venturelli Andrea 
Published: March 2020

Purpose
The aim of the paper is to understand the differences between “talking” and “walking” about sustainable development goals (SDGs) in state-owned enterprises (SOEs). Specifically, the authors have conducted an analysis on 202 entities that operate within the Italian National Healthcare System (INHS) to evaluate the overall degree of transparency in term of contribution to the SDG3.

Design/methodology/approach
The research evaluates the degree of contribution to SDG3 by INHS through the adoption of the theoretical framework proposed by Beck et al. (2010). Specifically, the authors assess the degree of contribution to this goal using an interpretive content analysis that combines the theoretical framework with the 13 targets that composed the SDG3. For the authors’ purposes, they analyze all INHS’s website to evaluate the presence/absence of social reports produced in the periods 2015-2018.

Findings
Although the great contribution to the SDG3, the INHS is characterized by a low degree of accountability. In fact, only 12.21 per cent of INHS’s entities disclosed at least one social report during the observed period. Moreover, the authors’ results denote how the approach of INHS’s entities to social reporting is different both in term of “quality” and “quantity.”

Research limitations/implications
The SOEs play a central role within the Agenda 2030 strategies. However, public managers are less oriented than private managers to adopt non-financial reporting tools. Furthermore, the authors’ results highlight the existence of asymmetric information between SOEs and citizens even if in presence of best practices such as the INHS. In this sense, the adoption of non-financial reports tool to engage in a more effective way with citizens could be a strategic driver for the achievement of highest degree of social legitimacy to operate.

Practical implications
The paper is of use to public managers operating in countries characterized by a high level of contribution to SDGs. Specifically, the authors’ results suggest how the adoption of reporting tools could impact positively in terms of stakeholder’s awareness to SDG themes.

Originality/value
This paper contributes to the understanding of the central role covered by academics, practitioners and public sectors to SDGs through the adoption of social reporting tools.
Developing a framework to revolutionise the 4D BIM process: IPD-based solution
Authors: Elghaish Faris , Abrishami Sepehr 
Published: March 2020

Purpose
The integration of building information modelling (BIM) and integrated project delivery (IPD) is highly recommended for better project delivery. Although there is a methodology for this integration, the BIM requires some improvements to foster the adoption of IPD. The purpose of this paper is to present an innovative way to support 4D BIM automation/optimisation within the IPD approach. Similar to structural and architectural design libraries, this research proposes a planning library to enable automating the formulation of schedule, as well as embedding the multi-objective optimisation into the 4D BIM.

Design/methodology/approach
The literature review was used to highlight the existing attempts to support the automation process for 4D BIM and the multi-objective schedule optimisation for construction projects. A case study was done to validate the developed framework and measure its applicability.

Findings
The results show that there is a cost-saving of 22.86 per cent because of using the proposed automated multi-objective optimisation. The case study shows the significance of integrating activity-based costing into 4D BIM to configure the hierarchy level of overhead activities with the IPD approach; therefore, the maximum level of contribution in managing the IPD project is 33.33 per cent by the trade package level and the minimum contribution is around 8.33 per cent by the project level.

Originality/value
This research presents a new philosophy to develop the 4D BIM model – planning and scheduling – a BIM library of the project activities is developed to enable the automation of the creation of the project schedule with respect to the 3D BIM design sequence. The optimisation of the project duration is considered to be automated within the creation process by using the proposed genetic algorithm model.
Don’t you (forget about me)
Authors: Mo Ce (Jacky) , Yu Ting , de Ruyter Ko 
Published: March 2020

PurposeTo advance research on channel relationship management, this study aims to test for the impacts of a channel member’s perception of exclusion from a supplier’s distribution channel networks (i.e. out-of-the-channel-loop perceptions [OCLP]) on supplier–channel partner relationships. The authors also systematically develop and empirically validate a scale to measure OCLP.Design/methodology/approachThis paper reports two empirical studies. The first develops a new scale for OCLP, following established approaches. The second tests the hypotheses. Survey data from a sample of channel firms operating in four industries were subjected to partial least squares modelling in the test of the hypothesized main and moderating effects.FindingsThe authors developed the new scale, including eight items, that capture OCLP from both social and economic perspectives. The results also show that OCLP has negative impacts on channel members’ psychological and behavioural outcomes (satisfaction, information sharing, positive word of mouth), after controlling for the effect of perceived unfairness. Channel partner perceived peer support emerges as a boundary condition of the impact; perceived informational support attenuates, whereas emotional support amplifies, the impact of OCLP.Research limitations/implicationsThis study suggests new research opportunities for explaining business-to-business marketing relationships using newly conceptualized OCLP.Practical implicationsThis study highlights that suppliers must recognize the potential for negative consequences of OCLP and manage these perceptions to minimize the negative implications. For suppliers, this study also offers several tools for managing OCLP.Originality/valueThis study introduces ostracism concepts to marketing channel literature to study a potential detriment to channel relationships. The proposed scale captures channel partners’ sense of exclusion from supplier relationships. It provides initial insights into the direct impacts on channel relational outcomes and associated boundary conditions.
Future study of operations research based on scenario planning and soft systems methodology
Authors: Fathi Mohammad Reza , Maleki Mohammad Hasan , Sobhani Seyed Mohammad , Koksal Can Deniz 
Published: March 2020

Purpose
The purpose of this study is to formulate exploratory scenarios of Operations Research through the critical uncertainty approach and Soft Systems Methodology.

Design/methodology/approach
In this study, to formulate plausible scenarios, the discipline of operation research internal and external experts’ opinions of this field have been gathered through Delphi approach and uncertainty questionnaires. After use of the most important uncertainties, plausible scenarios of operations research have been mapped with the help of experts through co-thinking workshops.

Findings
Four scenarios are presented in this study. These scenarios include Solar System, Esfandiar's Eye, Rival’s Setraps and Legendary Simurgh. Naturally, the imagination of such a unitary future for all academic communities is an expectation far from reality, and given the conditions of each of these futures or any integration of them is imaginable.

Originality/value
Operations Research models have been faced with variously multiple changes since its emergence until now. Investigation into the future of operations research on the necessity for his planning has not received a reasonable notice in the literature. Sporadic activities that have been carried out are also lacking in the necessary methodology. Also, there has been no research about future study using the soft Operation Research tools (Soft Systems Methodology).
Preventing supplier non-conformance: extending the agency theory perspective
Authors: Shevchenko Anton , Pagell Mark , Lévesque Moren , Johnston David 
Published: March 2020

PurposeThe supply chain management literature and agency theory suggest that preventing supplier non-conformance—a supplier's failure to conform to the requirements of the buyer—requires monitoring supplier behavior. However, case studies collected to explore how buyers monitored suppliers revealed an unexpected empirical phenomenon. Some buyers believed they could prevent non-conformance by either trusting their suppliers or relying on a third party, without monitoring their behavior. The purpose of this article is to examine conditions when buyers should monitor supplier behavior to prevent non-conformance.Design/methodology/approachThis article employs a mixed-method design by formulating an agent-based simulation grounded in the case-study findings and agency theory to reconcile observed unexpected behaviors with scholarly suggestions.FindingsThe simulation results indicate that buyers facing severe consequences from non-conformance should opt to monitor supplier behavior. Sourcing from trusted suppliers should only be reserved for buyers that lack competence and have a small number of carefully selected suppliers. Moreover, buyers facing minor consequences from non-conformance should generally favor sourcing from trusted suppliers over monitoring their behavior. The results also suggest that having a third-party involved in monitoring suppliers is an effective path to preventing non-conformance.Originality/valueBy combining a simulation with qualitative case studies, this article examines whether buyers were making appropriate decisions, thereby offering contributions to theory and practice that would not have been possible using either methodological approach alone.
Savouring place: cheese as a food tourism destination landmark
Authors: Fusté-Forné Francesc 
Published: March 2020

PurposeCheeses convey the identity of a region. The origin of milk, the pastures, the land, the cheesemakers and the traditional recipes transmit the ways a cheese is produced and consumed. This background links to food tourism practices, particularly to cheese-oriented tourism. In this sense, people can buy cheese at a range of selling points, and markets are being one of the most appreciated social, leisure and experience spaces. From a tourist and marketing perspective, the purpose of this paper is to explore the relationship between cheese consumption and place identity, drawing from a study into the cheeses sold in a medium-sized European city.Design/methodology/approachDrawing on 17 semi-structured interviews with cheese vendors in markets and specialist food shops, the provenances of cheeses sold in the city of Reus – south-eastern Catalonia, northeastern Spain – were studied.FindingsLocal, national and international cheeses were analysed. Results show the type of identity that derives from cheese offer and its opportunities for food tourism planning and development.Practical implicationsOutcomes of this paper may lead cheese producers to explore new arenas of cheesemaking and cheese distribution. Also, the results inform food tourism stakeholders of what consumers – both locals and tourists – gather when they buy cheese in a medium-sized European city.Originality/valueThis research offers an innovative approach to the study of the links between food and place. Based on the understanding of the origin of products, this paper leads to further comprehension of specialist food tourisms from the offer perspective, which may also drive to the development of a more robust destination gastronomic identity – in this case, through the particular provision of cheeses.