bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of Combined Warmed Preoperative Forced-air and Warmed Perioperative Intravenous Fluids on Maternal Temperature During Cesarean Section: a prospective, randomized, controlled clinical trial
Authors: Ni tingting , Zhou Zhen-feng , He Bo , Zhou Qing-he 
Published: February 2020

Abstract
Background: Preventing the frequent perioperative hypothermia incidents that occur during elective caesarean deliveries would be beneficial. This trial aimed at evaluating the effect of preoperative forced-air warming alongside perioperative intravenous fluid warming in women undergoing cesarean sections under spinal anesthesia. Methods: We randomly allocated135 women undergoing elective cesarean deliveries to either the intervention group (preoperative forced-air and intravenous fluid warmings, n = 69) or the control group (no active warming, n = 66). The primary outcome measure was the change from the core baseline temperature to that at the end of the procedure. Secondary outcomes included thermal comfort scores, the incidences of shivering and hypothermia (<36℃), the core temperature on arrival at the post-anesthesia care unit, neonatal axillary temperature at birth, and Apgar scores. Results: Two-way repeated measures ANOVA revealed significantly different core temperature changes (from the pre-spinal temperature to that at the end of the procedure) between groups ( F = 13.022, P <0.001). The thermal comfort scores were also higher in the intervention group than in the control group ( F = 9.847, P = 0.002). The overall incidence of perioperative hypothermia was significantly lower in the intervention group than in the control group (20.6% vs. 51.6%, P <0.0001). Conclusions: Warming preoperative forced-air and perioperative intravenous fluids may prevent maternal hypothermia, reduce maternal shivering, and improve maternal thermal comfort for patients undergoing cesarean sections under spinal anesthesia. Trial registration: The study was registered with the Chinese Clinical Trial Registry (registration number: ChiCTR1800019117) on October26, 2018. Keywords: Cesarean section, Spinal anesthesia, Warming
Associations of leptin and adiponectin with incident type 2 diabetes and interactions among African Americans: the Jackson Heart Study
Authors: Bidulescu Aurelian , Dinh Paul C. , Sarwary Shabir , Forsyth Emily , Luetke Maya C. , King David B. , Liu Jiankang , Davis Sharon K. , Correa Adolfo 
Published: February 2020

Abstract
Background: Growing evidence suggests that leptin is critical for glycemic control. Impaired leptin signaling may also contribute to low adiponectin expression in obese individuals. We assessed the association of leptin and adiponectin with incident type 2 diabetes (T2D), their interactions with sex and obesity status, and mediation by insulin resistance.Methods: Study participants from the Jackson Heart Study, a prospective cohort of adult African Americans in Jackson, Mississippi, included those free of T2D at the baseline Exam 1. Incident T2D was defined as new cases at Exam 2 or Exam 3. We created separate Cox regression models (hazard ratios per log-transformed ng/mL of leptin and adiponectin) with and without insulin resistance, HOMA-IR. Mediation by insulin resistance was analyzed. Several interactions were assessed, including by sex, HbA1c, and obesity.Results: Among 3,363 participants (mean age 53 years, 63% women), 584 developed incident T2D. Leptin was associated with incident T2D when modeled without HOMA-IR (HR=1.29, 95% CI=1.05-1.58). Among men, this positive association between leptin and T2D was significant (HR=1.33, 95% CI=1.05-1.69), but it was nonsignificant among women (HR=1.24, 95% CI=0.94-1.64); statistical interaction with sex was nonsignificant (p=0.65). The associations in all participants and in men were nullified by HOMA-IR (HR=0.99, 95% CI=0.80-1.22; HR=1.00, 95% CI=0.78-1.28, respectively), indicating mediation through insulin resistance (proportion mediated: 1.04), and were not observed in abdominally obese participants. Adiponectin was inversely associated with T2D even after adjustment for HOMA-IR in women (HR=0.68, 95% CI=0.55-0.84), but not in men (HR=0.80, 95% CI=0.62-1.04). The inverse association was present only among abdominally obese participants, and persisted after adjustment for HOMA-IR.Conclusions: Among African Americans in the Jackson Heart Study the association of leptin with incident T2D was explained by HOMA-IR. The association of leptin and incident T2D was mediated by insulin resistance and observed only among abdominally non-obese. Differences by sex appeared: men showed a significant association mediated by insulin resistance. Among abdominally obese participants, adiponectin was inversely associated with incident T2D even after adjustment for HOMA-IR.
Immunogenicity analysis of conserved fragments in Plasmodium ovale species merozoite surface protein 4
Authors: Juliette Uwase , Chu Ruilin , Kassegne Kokouvi , Lei Yao , Shen Feihu , Fu Haitian , Sun Yifan , Xuan Yinghua , Cao Jun , Cheng Yang 
Published: February 2020

Abstract
Background: There is an urgent need for an effective vaccine to control and eradicate malaria, one of the most serious global infectious diseases. Plasmodium merozoite surface protein 4 (MSP4) has been listed as a blood-stage subunit vaccine candidate for malaria. Plasmodium ovale species (spp.) infection is also a source of malaria burden in tropical regions where it is sometimes mixed with other Plasmodium species. However, little is known about P. ovale spp. MSP4.
Methods: The msp4 gene was amplified through polymerase chain reaction using genomic DNA extracted from blood samples of 46 patients infected with P. ovale spp. and amplified products were sequenced. Open reading frames predicted as immunogenic peptides consisting of 119 and 97 amino acids of P. ovale curtisi MSP4 (PocMSP4) and P. ovale wallikeri MSP4 (PowMSP4), respectively, were selected for protein expression. Recombinant proteins (rPoMSP4) were expressed in Escherichia coli, purified, analyzed, and immunized in BALB/c mice. The specificity of anti-MSP4-immunoglubulin (Ig) G antibodies was evaluated by Western blot and enzyme-linked immunosorbent assays, and cellular immune responses were analyzed via lymphocyte proliferation assays.
Results: Full peptide sequences of PocMSP4 and PowMSP4 were completely conserved in all clinical isolates, except in the epidermal growth factor-like domain at the carboxyl terminus where only one mutation was observed in one P. ovale wallikeri isolate. Furthermore, we successfully expressed the truncated pocmsp4 and powmsp4 and purified as ~32 kDa proteins. Our results showed that PocMSP4 and PowMSP4 induced high antibody responses with end-point titers ranging from 1:10,000 to 1:2,560,000 in all immunized mouse groups and with high IgG avidity to PocMSP4 (80.5%) and PowMSP4 (92.3%). Furthermore, rPocMSP4 and rPowMSP4 cross-reacted with anti-PowMSP4-specific or anti-PocMSP4-specific antibodies. Additionally, anti-PoMSP4 IgG antibodies showed broad immuno-specificity in reacting against rPoMSP1 and rPoAMA1. Lastly, PocMSP4- and PowMSP4-immunized mice induced cellular immune responses with PocMSP4 (36%) and PowMSP4 cells (15.8%) as observed through splenocyte proliferation assays.
Conclusion: Our study suggested conservation in PoMSP4 protein sequences and high immunogenicity was observed in rPoMSP4. Furthermore, PocMSP4- and PowMSP4-immunized mice induced cellular immune responses, suggesting that both humoral and cellular immune responses play crucial roles in the protection against any antigen.
Network-based identification of biomarkers for colon adenocarcinoma
Authors: Hu Fuyan , Wang Qing , Yang Zhiyuan , Zhang Zeng , Liu Xiaoping 
Published: February 2020

Abstract
As one of the most common cancers with high mortality in the world, we are still facing a huge challenge in the prevention and treatment of colon cancer. In this study, we firstly constructed a functional network for colon adenocarcinoma (COAD) by using a sample-specific network (SSN) method which can construct individual-specific networks based on gene expression profiles of a single sample. The results of classification and enrichment analysis showed that the identified genes could be used as diagnosis biomarkers. Finally, 12 functional genes and 13 functional interactions in the functional network could serve as prognosis biomarkers since they were associated with the survival rate of COAD.
Collaborative subcellular compartmentalization to improve GPP utilization and boost sabinene accumulation in Saccharomyces cerevisiae
Authors: Jia Hongjie , Chen Tianhua , Qu Junze , Yao Mingdong , Xiao Wenhai , Wang Ying , Li Chun , Yuan Yingjin 
Published: February 2020

Abstract
Background Monoterpenes and their derivatives play an important role as flavorings, perfume additives, pharmaceuticals and advanced biofuels. GPP (geranyl diphosphate) is the direct precursor to biosynthesize this class of products. The present study for monoterpenes synthesis in yeast focused on manipulation of metabolic flux to improve GPP supply. However, if the subcellular distribution of GPP and monoterpene synthase were not coincided with each other, it would be hard to well utilize GPP, leading to a waste of carbon sources.Results Herein, we took sabinene production in Saccharomyces cerevisiae as an instance, and confirmed the location of N-truncated sabinene synthase (t34SabS1) in yeast cytosol (C). We also revealed the existence of GPP pools in organelles [such as lipid monolayer membrane-bound peroxisomes (P) and bilayer membrane-bound mitochondria (M)] beside cytosol. In order to minimize the loss of GPP, an engineering strategy was proposed to coordinated compartmentalization of sabinene synthase. Initially, expression of t34SabS1 in an ERG20-downregulated host only obtained 19.4 mg/L sabinene. Combined targeting t34SabS1 into CM (cytosol and mitochondria) increased sabinene production to 64.6 mg/L. This titer was significantly higher than those generated by harnessing other combinations of subcellular locations ( i.e. CP, PM and even CPM). Further overexpression of the genes involved in mitochondria morphology uncovered four novel molecular targets ( i.e. FIS1 , LSB3 , MBA1 and AIM25 ) associating with sabinene output. Especially, overexpression of AIM25 enhanced the sabinene production to 90.4 mg/L. Eventually, integrating all above engineered genes into host chromosome achieved 154.9 mg/L of sabinene.Conclusions The engineering approaches of this study improve GPP utilization and boost sabinene accumulation almost 60-fold of the original titer. This research highlights the strategy of organelle engineering to improve precursor utilization and to enhance the compartmentalized pathway. It also sets a good reference to synthesize other valuable monoterpenes and their derivatives in eukaryotic hosts.
Information communication texnolgy for monitoring of a prenosological state of the population health&nbsp;
Authors: Abdumanonov Akhrorjon , Karabaev Muhammadjon 
Published: February 2020

Abstract
Background: The article describes the development of an uninterrupted functioning technology for remote automated monitoring of the prenosological level of individual health, which allows optimizing the medical non-invasive mass examination of the population. Methods:In order to provide their functioning by using information and communication technologies, mobile communications, automated remote exchange methods, accumulation, storage of information, as well as involving medical knowledge, mathematical models and algorithms for their intellectual analysis, the opportunities have been created for the development and implementation of preventive measures that are effective mechanisms to ensure a healthy lifestyle. Results: According to the stated goal, a comprehensive monitoring of the physical, functional and clinical-somatic status of population health indicators is carried out. It provides the implementation of the functions of a comprehensive prenosological examination of citizens and the results of the surveys assess the risk of developing certain diseases; assisting in the implementation of measures to form a healthy lifestyle and reduce the spread of risk factors for noncommunicable diseases among the attached population; dynamic monitoring of the contingent of individuals at increased risk of developing the disease. The practical use of this technology helps to conduct mass prenosological examinations of the population and improve the quality of targeted, personalized prevention of risk factors and improve their health.
Therapeutic effect of acetabular fractures using the Pararectus approach combined with 3D printing technique
Authors: Zou Ruyi , Wu Min , Guan Jianzhong , Xiao Yuzhou , Chen Xiaotian 
Published: February 2020

Abstract
Background To explore the clinical efficacy of pararectus approach combined with 3D printing technique for the surgical treatment of partial complex acetabular fractures. Methods We retrospectively evaluated 18 (11 males and 7 females) patients with complicated acetabular fractures in the period of June 2017 to December 2018; According to judet-letenneal classification: 11 cases were of double column fracture, 6 fractures were of the anterior column with posterior half transverse, and 1 case was of "T" fracture. For all cases, 3D printing is used to print the acetabular model. Pre-bent reconstruction plates from the model were placed to fixate fractures after reduction via the pararectus approach. Results The average time of surgery was 203 min (range:135-245 min)and mean intraoperative blood loss was 1030ml(range:450-1400ml);18 patients were followed up for 12-18 months (average,14 months);One patient (5.6%) developed postoperative wound infection, and the wound completely improved by secretion culture, enhanced dressing and effective antibiotics. One patient (5.6%) developed postoperative ossifying myositis, and there are no obvious symptoms at present; all patients underwent pelvic films and pelvic CT+ three-dimensional reconstruction after surgery, suggesting that fractures reduction as well. All the acetabular fractures united after 12 to 16 weeks(average,13 weeks);According to the modified Merle d’Aubigne and Postel scoring system to assess the hip function: excellent in 13 cases (72.2%), good in 3 cases(16.7%) and fair in 2 cases(11.1%). Conclusions In the treatment of partial complex acetabular fractures, the pararectus approach combined with 3D printing technique can achieve effective reduction and fixation, decrease intraoperative hemorrhage, shorten operation time and the internal fixation position can be properly adjusted during the operation by looking directly at the model.
Preliminary clinical and radiographic outcomes of oblique lateral interbody fusion combined with percutaneous transforaminal endoscopic discectomy for degenerative lumbar disease accompanied by prolapsed disc herniation
Authors: Lou Chao , Liu Shijie , Yu Weiyang , Liu Feijun , Chen Zhenzhong , Gao Jiawei , Pan Bin , He Dengwei 
Published: February 2020

Abstract
Objective: To investigate the preliminary clinical and radiographic outcomes of oblique lateral interbody fusion (OLIF) combined with percutaneous transforaminal endoscopic discectomy (PTED) for the degenerative lumbar disease accompanied by prolapsed disc herniation.Methods: From March 2016 to December 2018, 15 patients with degenerative lumbar spondylolisthesis, lumbar spine instability or lumbar spinal stenosis accompanied by prolapsed disc herniation were underwent OLIF combined with PTED in our spine surgery center, including 6 males and 9 females, the mean age was 61.4±7.1 years. Clinical results, radiological parameters, and related complications were collected and analyzed.Results: All patients firstly received PTED with local anesthesia and then underwent OLIF with general anesthesia. All patients were followed up for an average of 14.6±3.7 months. Mean preoperative visual analog scale scores and Oswestry Disability Index scores were significantly improved postoperatively (P<0.05). The radiographic results include the intervertebral disc height, vertical diameter of intervertebral foramen, spinal canal anteroposterior diameter, segment angle and lumbar lordosis angle were significantly improved postoperatively, respectively (P<0.05). None major implant-related and other severe complications were happened.Conclusions: OLIF combined with PTED can successfully enables direct neural decompression without posterior decompressive procedures and might be an alternative minimally invasive surgical option for properly selected patients.
Identifying the novel key genes in renal cell carcinoma by bioinformatics analysis and cell experiments
Authors: Chen Yeda , Gu Di , Wen Yaoan , Yang Shuxin , Duan Xiaolu , Lai Yongchang , Yang Jianan , Yuan Daozhang , Khan Aisha , Wu Wenqi , Zeng Guohua 
Published: February 2020

Abstract
Background: Although major driver gene have been identified, the complex molecular heterogeneity of renal cell cancer (RCC) remains unclear. Therefore, more relevant genes need to be identified to explain the pathogenesis of renal cancer.Methods: Microarray datasets GSE781, GSE6344, GSE53000 and GSE68417 were downloaded from Gene Expression Omnibus (GEO) database. The differentially expressed genes (DEGs) were identified by employing GEO2R tool, and function enrichment analyses were performed by using DAVID. The protein-protein interaction network (PPI) was constructed and the module analysis was performed using STRING and Cytoscape. Survival analysis was performed using GEPIA. Differential expression was verified in Oncomine. Cell experiments (cell viability assays, transwell migration and invasion assays, wound healing assay, flow cytometry) were utilized to verify the roles of the hub genes on the proliferation of kidney cancer cells (A498 and OSRC-2 cell lines). Results: A total of 215 DEGs were identified from four datasets. Six hub gene (SUCLG1, PCK2, GLDC, SLC12A1, ATP1A1, PDHA1) were identified and the overall survival time of patients with RCC were significantly shorter. The expression levels of these six genes were significantly decreased in six RCC cell lines(A498, OSRC-2, 786-O, Caki-1, ACHN, 769-P) compared to 293t cell line. The expression level of both mRNA and protein of these genes were downregulated in RCC samples compared to those in paracancerous normal tissues. Cell viability assays showed that overexpressions of SUCLG1, PCK2, GLDC significantly decreased proliferation of RCC. Transwell migration, invasion, wound healing assay showed overexpression of three genes(SUCLG1, PCK2, GLDC) significantly inhibited the migration, invasion of RCC. Flow cytometry analysis showed that overexpression of three genes(SUCLG1, PCK2, GLDC) induced G1/S/G2 phase arrest of RCC cells. Conclusion: Based on our current findings, it is concluded that SUCLG1, PCK2, GLDC may serve as a potential prognostic marker of RCC.
Identification of genes associated with lymph node metastasis in papillary thyroid carcinoma by weighted gene co-expression network analysis
Authors: Liu Zhen , Guo Mingyue , Wang Lidong , Liu Chenxi , Huang Yonglian 
Published: February 2020

Abstract
Background: Thyroid cancer (TC) is the most common endocrine malignant tumor, in which thyroid papillary carcinoma has the highest incidence of (PTC). However, some patients will relapse or even die because of tumor metastasis. Therefore, for the treatment of PTC, it is extremely important to find gene targets and their potential biomarkers. We obtained the RNA sequence matrix of PTC and the clinical information of the corresponding samples in the TCGA database. Methods: The weighted gene co-expression network analysis was utilized to construct the co-expression network, and the gene modules with high correlation among genes and the relationship between genes and clinical traits were established. Results: We utilized the screened 5271 genes for WGCNA analysis and constructed 19 gene modules. Genes in the magenta module with the highest correlation with lymph node metastasis were selected for enrichment analysis to construct a protein interaction network. After that, we selected 12 hub genes of this module by using the intersection of the differential genes in the data set of cytoscape and GEO database. 12 genes were visualized in GEPIA. After that, the gene expression was verified on the oncomine page, and 8 genes with high correlation with TC lymph metastasis (ARNTL, CDH3, CLDN1, COL8A1, COL8A2, IL1RAP, LPAR5) were screened. Conclusion: In this paper, the differential genes are screened by combining the datasets of TCGA, GEO and Oncomine in TC for the first time, which makes the screened differential genes have more biological significance. These genes may offer new insights for the treatment of PTC lymph node metastasis.
Novel cyclic peptides from lethal Amanita species through a genomic approach, and major peptide toxins in the ectomycorrhizal association
Authors: Luo Hong , Zhou Shengwen , Li Xincan , Li Xuan , Chen Zuo H. , Yang Zhu L. , Li Guohong 
Published: February 2020

Abstract
Background: Most species in the genus Amanita are ectomycorrhizal fungi, and the cyclic peptide toxins that some species produce are notoriously deadly. In total, around 25 of these peptides were found in the fruiting bodies over the past 82 years, and whether any of them are present in the mycorrhizae is unknown. Reportedly, sequenced lethal Amanita genomes harbor a significant number of precursor genes of MSDIN family, indicating there could be a much larger capacity for cyclic peptide production in these mushrooms. However, it is largely unknown that to what extent these genes are transcribed, and further, translated into true cyclic peptides.Method: In this study, three poisonous Amanita species, A. rimosa, A. exitialis and A. subjunquillea, were sequenced through PacBio and Illumina techniques. For expression analysis, one strain of A. subjunquillea was sequenced through RNA-Seq. A genome-guided approach was adopted to identify cyclic peptides by coupling predicted toxin-biosynthetic genes with mass spectrometry (MS and MS/MS). To investigate whether any of the toxins were express in the microbiome, profiling of known major toxins was conducted on A. subjunquillea mycorrhizae via HRMS and gene cloning.Results: The resultant genomes showed significant potential to produce known and unknown cyclic peptides. Together with our 2 previously sequenced genomes, in total 37 unknown MSDIN genes were discovered. Expression of over 90% of the MSDIN genes was demonstrated in two strains of A. subjunquillea. Through the genome-guided approach, 12 MSDIN genes were found to produce true, novel cyclic peptides with no additional posttranslational modifications. When the ectomycorrhizae of A. subjunquillea were analyzed by MS, all major toxins were detected. The corresponding MSDINs for these cyclic peptides were successfully cloned directly from the mycorrhizae.Conclusions: The genome-guided approach provided a speedy method to identify cyclic peptides both in Amanita mushrooms and in the ectomycorrhizae. In this study, a significant number of novel MSDIN genes were discovered, most of which were found to be expressed in the tested species. The identification of the 12 novel cyclic peptides strongly suggests that Amanita species possess a much larger reservoir of these peptides than previously thought. This is the first report to demonstrate that the cyclic peptides in Amanita species are expressed in the mycorrhizal association. All four major toxins, α-amanitin, β-amanitin, phallacidin and phalloidin, are found to be present in the symbiosis, offering new clues to their biological function(s).