bluuebunny/crossref_metadata_2025_split huggingface dataset:
WITHDRAWN: Interplay between cancer cells and M2 macrophages is necessary for miR-550a-3-5p down-regulation-mediated HPV-positive OSCC progression
Authors: Cao Mingxin , Zhang Weilong , Yu Xianghua , Wu Jiashun , Qiao Xinwei , Huang Meichang , Wang Ke , Wu Jingbiao , Tang Yajie , Jiang Jian , Liang Xinhua , Tang Ya-ling 
Published: May 2020

Abstract

Background: Human papillomavirus (HPV)-positive oral squamous cell carcinoma (OSCC) is increasing worldwide with typically higher grade and stage, while better prognosis. microRNAs (miRNAs) has been shown to play a critical role in cancer, however, their role in HPV-positive OSCC progression remains unclear.Methods: miRNA microarray was performed to identify differentially expressed miRNAs. qRT-PCR and FISH were performed to determine the relative expression of miR-550a-3-5p. CCK-8, Flow cytometry, Wound healing, Cell invasion assays and xenograft experiments were conducted to analyze the biological roles of miR-550a-3-5p. Tumor-associated macrophages (TAMs) generation, co-culturing of cancer cells with TAMs, Western blot, Dual-luciferase reporter gene assay, Immunohistochemistry and animal studies were performed to explore the mechanisms underlying the functions of miR-550a-3-5p.Results: We identified 19 miRNAs differentially expressed in HPV-positive OSCC specimens and miR-550a-3-5p was down-regulated. The low expression of miR-550a-3-5p correlated with higher tumor size and nodal metastasis of HPV-positive OSCC patients. Then, we found that miR-550a-3-5p suppressed the migration, invasion and EMT of HPV-positive OSCC cells dependent on decreasing M2 macrophages polarization. Moreover, miR-550a-3-5p, down-regulated by E6 oncoprotein, inhibited M2 macrophages polarization by YAP/CCL2 signaling, which in turn abrogating EMT program in HPV-positive OSCC cells. In addition, in both xenografts and clinical HPV-positive OSCC samples, miR-550a-3-5p levels were inversely associated with YAP, CCL2 expressions and the number of M2 macrophages.Conclusions: E6/miR-550a-3-5p/YAP/CCL2 signaling induces M2 macrophages polarization to enhance EMT and progression, revealing a novel crosstalk between cancer cells and immune cells in HPV-positive OSCC microenvironment.
LncRNA sequencing analysis of cervical cancer identifies radiotherapy sensitivity markers
Authors: Zhang Xinqiang , Ma Changsheng , Dong Xinyu , Liu Cuihua , Jin Shunzi , Wang Zhicheng , Sun Baosheng , Shen Yannan 
Published: May 2020

Abstract
Background

Cervical cancer is one of the most common human cancers worldwide. However, the treatment of cervical cancer does not meet patient expectations. In recent years, long noncoding RNAs (lncRNAs), which are nonprotein-coding transcripts, have been shown to play important roles in tumorigenesis and tumor progression.
Methods

The function and mechanism of lncRNAs in cervical carcinoma are still rarely reported. We selected 15 patients with cervical cancer and a good prognosis (RS) and 15 patients with cervical cancer and a poor prognosis (RR), and we then performed lncRNA Sequencing analysis to detect differentially expressed lncRNAs. Subsequently, we used online bioinformatics databases to explore the potential function of these dysregulated lncRNAs. Finally, quantitative real-time PCR was performed to confirm the expression levels of these dysregulated lncRNAs in sub-RR and RS samples.
Results

We used lncRNA Sequencing technology to reveal differentially expression between the lncRNA profiles of the RR and RS tissues, and we found 1097 upregulated and 1189 downregulated lncRNAs in cervical cancer (fold change > 2.0), 247 upregulated and 189 downregulated lncRNAs in cervical cancer (fold change > 5.0), and 94 upregulated and 95 downregulated lncRNAs in cervical cancer (fold change > 10.0).
Conclusions

This study provides a map of lncRNA expression in RR and RS tissues and provides a database for further study of the function and mechanisms of action of key lncRNAs in cervical cancer. LncRNA TCONS_00081487 regulate CD55 and increase the radiation sensitivity of cervical cancer.
Formulation a modern chemotherapeutic drug of Ziziphora clinopodides Lam leaf aqueous extract conjugated cadmium nanoparticles and analysis of their anti-acute myeloid leukemia potentials in comparison to Daunorubicin in leukemic mouse and cellular models: Antidiabetic and Anticholinergics Potentials
Authors: Mahdavi Behnam , Zangeneh Mohammad Mahdi , Paydarfar Sogand , Rezaeiseresht Esmaeil , Zangeneh Akram , Sadeghian Nastaran , Taslimi Parham , Alkac Ismail Mert , Şen Fatih 
Published: May 2020

Abstract
In this study, the anti-acute myeloid leukemia (AML) effects of Ziziphora clinopodioides Lam leaf aqueous extract conjugated cadmium nanoparticles (CdNPs) were compared with daunorubicin as a common chemotherapeutic drug. To synthesize CdNPs, Z. clinopodioides aqueous extract was combined with Cd(NO 3 ) 2 .4H 2 O. For analyzing CdNPs, SEM, UV-Vis. spectroscopy, X-ray powder diffraction and Energy dispersing X-ray spectroscopy, Fourier Transform Infrared techniques were used. In FE-SEM images of CdNPs, the diameter of particle dimension was on means of 26.78 nm. To survey the cytotoxicity and anti-AML effects of Cd(NO 3 ) 2 , Z. clinopodioides, Daunorubicin, CdNPs and MTT assay was used on the human AML cell lines. The IC50 of the cadmium nanoparticles was 168, 205 and 210 µg/mL against Murine C1498, 32D/FLT3-ITD and Human HL-60/vcr cell lines, respectively. In vivo study, CdNPs like daunorubicin ameliorated importantly (P≤0.01) the biochemical, WBC, RBC, inflammatory, platelet, stereological, histopathological and cellular-molecular parameters compared to the other groups. As mentioned, the cadmium nanoparticles had significant anti-AML effects. After approving the above eventuate in the clinical trial studies, these cadmium nanoparticles could be used in humans as a chemotherapeutic medicament for the treatment of AML. Additionally, the novel nanoparticles (Cd(NO 3 ) 2 and CdNPs) were good inhibitors of the α-glycosidase, and cholinesterase enzymes.
New Laboratory Criterion for the Differential Diagnosis of Sarcoidosis and Pulmonary Tuberculosis
Authors: Starshinova Anna , Malkova Anna , Zinchenko Yulia , Basantsova Natalia , Kudryavtsev Igor , Lapin Sergey , Mazing Alexandra , Surkova Elena , Pavlova Maria , Belyaeva Ekaterina , Stepanenko Tatiana , Yablonskiy Piotr 
Published: May 2020

Abstract
Take-Home Message.This study provides a new diagnostic index based on the antibodies to mutated citrullinated vimentin concentration and the level of different B subsets. Determination of index over 5 Units proved to be characteristic of sarcoidosis, what might help to differentiate SD from TB.Abstract. In some cases there is a problem of differential diagnosis of sarcoidosis(SD) and tuberculosis(TB)because of the similarities in clinical, X-ray and laboratory features. The aim of this study was to search for new differential diagnostic criteria for sarcoidosis and tuberculosis by calculating the index, based on the level of autoantibodies to modified citrullinated vimentin (anti-MCV) and the level of B-cell subpopulations. These parameters were measured in patients with sarcoidosis (n = 93), tuberculosis (n = 40) and healthy donors (n = 40) using the ELISA and cytometry. The absence of a statistically significant difference when comparing the level of anti-MCV, the number of B-cells in SD and TB suggests that these changes may be characteristic of granulomatous diseases. The use of the formula Ds=([B-naïve%]\[B-memory%])*([B-CD38%]+[B-CD5%])/[anti-MCV] might allow to differentiate SD with an increase in the calculated index of more than 5 units with a sensitivity of 80.00% and specificity of 93.10% (AUC = 0.926).
Differential Expression of Gluconeogenic Enzymes in Early- and Late- Stage Diabetes: The Effect of Citrullus Colocynthis Seed Extract on Hyperglycemia and Hyperlipidemia
Authors: Afshari Arezoo , Salimi Fouzieh , Babaie Masumeh , Bakhtiyari Salar , Hassanzadeh Gholamreza , Shabani Maryam , Nowrouzi Azin 
Published: May 2020

Abstract
Background: The extent of metabolic disruption and the usefulness of drugs and supplements, such as medicinal plants, in patients with diabetes may be impacted by the severity of the disease. Method: Nicotinamide + STZ together and STZ alone were used to induce early- and late-stage diabetes type 2 (ET2D and LT2D, respectively) in rats. Glucose tolerance test (GTT) was performed because the stage of disease was considered compatible with the magnitude of deviation from normal glucose tolerance test (GTT), as well as the level of FBS. Three main groups of the study were nondiabetic, early-stage diabetic, and late-stage diabetic rats. Each group was divided into two sub-groups, one of which received Citrullus colocynthis seed aqueous extract (CCAE, 90mg/kg BW) for 28 days. Weekly FBS and body weights were recorded during the study. At the end of 28 days, the serum levels of ALT, AST, ALP, TG, urea, creatinine, uric acid, cholesterol, HDL, LDL, c-peptide, and HbA1c were measured; the hepatic mRNA expression of several enzymes of glucose and fat metabolic pathways were also determined by Real-Time PCR. The accumulation of fat in the hepatic tissue was visualized by measuring the TG content and by H&E and Oil-Red staining and the degree of oxidative stress was measured by protein carbonyl content (PCC).Results: The LT2D rats showed maximal deviations from normal GTT. GTT for control and ET2D rats were similar, yet the area under curve (AUC) for ET2D rats was significantly higher. Urea, ALT, and ALP levels were high in diabetic rats compared to control and significantly different from each other.Serum TG dropped and ALT, ALP, HDL and LDL significantly improved after treatment with CCAE. Different patterns of G6Pase and PEPCK expression in ET2D and LT2D suggested their similarity to short- and long-term fasting states, respectively. While the reduction of FBS levels in ET2D could be explained by an inhibition of G6Pase expression and therefore glycogenolysis, the level of PEPCK expression was not significantly lowered by CCAE in LT2D. H&E staining of liver tissue showed steatoses of varying morphology in both ET2D and LT2D rats. CCAE led to up-regulated PPARα and down-regulated CPT1 expressions.Conclusion: As PEPCK activity is necessary for the continuation of the TG/FA cycle during fasting, it is possible that in LT2D, CCAE directed the PEPCK activity more towards glyceroneogenesis than gluconeogenesis to ensure the persistence of the TG/FA cycle. The enhanced glyceroneogenesis together with an up-regulated PPARa expression and down-regulated CPT1 expression probably led to lower blood and hepatic TG. More research is needed to establish the effect of CCAE on PEPCK expression and its course of activity.
The Gardening Activities to Reduce Postpartum Stress in Japanese Postpartum Women: Preliminary Evidence from Pilot Single-Arm Trial Study
Authors: Kotozaki Yuka 
Published: May 2020

Abstract
Objective

There is a need for safer and more effective programs to address postpartum women's mental health dysfunction. The objective of this study was to clarify the effects on mental health, including postpartum depression, by implementing Japanese postpartum women with gardening activities by a pilot single-arm trial.
Results

15 women about a year after giving birth, recruited through newspaper advertisements, took part in this pilot study. I used the Japanese version of Edinburgh Postpartum Depression Scale (EPDS) score as the primary outcome measure. Secondary outcome measures were parenting stress, anxiety, and quality of life (QOL). Data collected at pre and post-test were analyzed using a paired t test. The EPDS, stress regarding parents, State-Trait Anxiety Inventory (STAI)-state, and STAI-trait of post-test were significant decreased than pre-test (p < 0.05). Also, physical health and psychological health of the World Health Organization Quality of life 26 (WHOQOL-26) of post-test were significantly increased than pre-test (p < 0.05). The gardening activity might be promising for mitigating depressive mood in postpartum women.
Effectiveness of Low-Frequency Stimulation in Proprioceptive Neuromuscular Facilitation Techniques for Post-Ankle-Sprain Balance and Proprioception in Adults- a Randomized controlled trial
Authors: ALAHMARI KHALID A , Samuel Paul Silvian , Ahmad Irshad , Reddy Ravi Shankar , Tedla Jaya Shanker , Kakaraparthi Venkata Nagaraj , Rengaramanujam Kanagaraj 
Published: May 2020

Abstract
BackgroundStretching is an important part of post-ankle-sprain rehabilitation, as well as an effective exercise for improving general ankle-joint performance. But the combination of stretching alongside muscle stimulation has not yet been extensively studied. Therefore the purpose of the present research is to compare the baseline, post- and follow-up effects of the proprioceptive neuromuscular facilitation (PNF) stretching technique combined with transcutaneous electrical nerve stimulation (TENS), as compared against the effects of the PNF stretching technique alone.MethodsSixty subjects with lateral ankle sprains were selected and randomly allocated to three groups: Experimental Group One (EG 1), Experimental Group Two (EG 2), and the Control Group (CG). Subjects in EG 1 received the PNF stretching technique combined with TENS. Subjects in EG2 received the PNF stretching technique alone. Both experimental groups received these treatments for 4 weeks (4 days/week); follow-up assessments were administered in the third and fifth weeks. CG received no treatment; outcome measures alone were assessed. Outcome measures comprised pain, flexibility, proprioception, range of motion, muscle strength, physical activity, and balance. A mixed-model ANOVA was used to analyze the effects of time factors and groups on these outcome measures.ResultsThere was significant interaction (time and group), and the time effect for all the outcome measures (p < 0.05). Physical activity, dorsiflexion, and balance in the medial, lateral, anterolateral, and anteromedial directions did not show a significant difference between the groups. EG 1 showed significant improvement for all the outcome variables between pre- and post-treatment and follow-up when compared to the other groups.ConclusionsThe present study showed that a 12-session treatment program of 3 weeks’ duration that combines PNF stretching with low-frequency TENS for post-ankle sprain subjects, compared against PNF stretching alone, produced significant improvements in balance, proprioception, strength, and range of motion. The study also showed that the treatment effect was sustained even after treatment was ceased after the follow-up assessment in the fifth week.Trial Registration:Human Research Ethics Committee approval for the trial (approval no.: (ECM#2019-26)Clinical trial was also registered in the Clinical Trials Registry – ISRCTN 18013941﻿
A Synergistic Bactericidal Effect of Low-Frequency and Low-Intensity Ultrasound Combined with Levofloxacin-Loaded PLGA Nanoparticles on M. Smegmatis in Macrophages
Authors: Xie Shuang , Li Gangjing , Hou Yuru , Yang Min , Li Fahui , Li Jianhu , Li Dairong , Du Yonghong 
Published: May 2020

Abstract
Purpose: Tuberculosis (TB) is a highly infectious disease caused by Mycobacterium tuberculosis (Mtb), which often parasites in macrophages. This study is performed to investigate the bactericidal effect and underlying mechanisms of low-frequency and low-intensity ultrasound (LFLIU) combined with levofloxacin-loaded PLGA nanoparticles (LEV-NPs) on M. smegmatis (a surrogate of Mtb) in macrophages.Methods and results: The LEV-NPs were prepared using a double emulsification method. The average diameter, zeta potential, polydispersity index, morphology, and drug release efficiency in vitro of the LEV-NPs were investigated. M. smegmatis in macrophages was treated using the LEV-NPs combined with 42 kHz ultrasound irradiation at an intensity of 0.13 W/cm2 for 10 min. The results showed that ultrasound significantly promoted the phagocytosis of nanoparticles by macrophages (p < 0.05). In addition, further ultrasound combined with the LEV-NPs promoted the production of reactive oxygen species (ROS) in macrophage , and the apoptosis rate of the macrophages was significantly higher than that of the control (p < 0.05). The transmission electronic microscope showed that the cell wall of M. smegmatis was ruptured, the cell structure was incomplete, and the bacteria received severe damage in the ultrasound combined with the LEV-NPs group. Activity assays showed that ultrasound combined with the LEV-NPs exhibited a 10-fold higher antibacterial activity against M. smegmatis residing inside macrophages compared with the free drug.Conclusion: These data demonstrated that ultrasound combined with LEV-NPs has great potential as a therapeutic agent for TB.
Health-related quality of life after pelvic organ prolapse surgery in Ethiopia: A 6 month follow-up study
Authors: Belayneh Tadesse , Gebeyehu Abebaw , Adefris Mulat , Rortveit Guri , Gjerde Janne Lillelid , Awoke Tadesse 
Published: May 2020

Abstract
Background
Pelvic organ prolapse (POP) affects health-related quality of life (HRQoL). Patient-reported outcomes (PRO) measures the quality of care from the patient’s perspective. PROs are an important measure of surgical outcome and used to calculate health gains after surgical treatment. We assessed HRQoL in women undergoing surgical repair of POP.
Methods
Two hundred fifteen women with stage III or IV prolapse underwent surgical POP repair between February 2018 and May 2019. Pelvic Organ Prolapse Quality of Life (P-QoL-20) was administered at baseline, 3 and 6 months postoperatively to assess HRQoL. Depressive symptoms and body image also evaluated. Linear mixed-effect models were used to compare pre and postoperative HRQoL scores and investigate potential predictors.
Results
Participant’s mean age was 49.3 ± 9.4 years. Most (81.9%) had stage III prolapse and underwent a vaginal hysterectomy, although 40% preferred uterine preservation. No differences were seen between women follow-up and those lost to follow-up in HRQoL (p > 0.05). The P-QoL, depressive symptoms, and body image were improved 6 months post-operatively. The change in P-QoL significantly associated with body image scores. The type of surgery did not show a significant difference. Being married showed an improvement in the personal relationship subscale score (β = 5.8, p < 0.01).
Conclusions
Our results indicated a potential improvement of HRQoL after surgical treatment. The result could be useful for patient counseling on the expected HRQoL outcomes of surgical treatment. Surgical service should be accessible for women suffering from POP to improve HRQoL.
Characterizing sensitivity and coverage of clinical WGS as a diagnostic test for genetic disorders
Authors: Sun Yan , Liu Fengxia , Fan Chunna , Wang Yaoshen , Song Lijie , Fang Zhonghai , Han Rui , Wang Zhonghua , Wang Xiaodan , Yang Ziying , Xu Zhenpeng , Peng Jiguang , Shi Chaonan , Zhang Hongyun , Dong Wei , Huang Hui , Li Yun , Le Yanqun , Sun Jun , Peng Zhiyu 
Published: May 2020

Abstract
Background
With the reduce of cost and incomparable advantages, WGS is likely to change the way of clinical diagnosis of rare and undiagnosed diseases. However, the sensitivity and breadth of coverage of clinical WGS as a diagnostic test for genetic disorders has not been fully evaluated, especially for CNV detection. All the samples underwent WGS using MGISEQ-2000. The performance of NA12878, YH cell line, and the Chinese trios were measured for sensitivity, PPV, depth and breadth of coverage. We also compared the performance of WES and WGS using NA12878. The sensitivity and PPV were tested using family-based trio design in the Chinese trios. We also developed a systematic WGS pipeline for the analysis of 8 clinical cases with known disease-causing variants.
Results
In general, the sensitivity and PPV for SNV/indel detection increased with mean depth, and reached a plateau at a ~ 40X mean depth using down-sampling samples of NA12878. With a mean depth of 40X, the sensitivity of homozyous and heterozygous SNPs of NA12878 was > 99.25% and > 99.50% respectively, and PPVs were 99.97% and 98.96%. Homozygous and heterozygous indels showed lower sensitivity and PPV. The sensitivity and PPV is still not 100% even with a mean depth of ~ 150X. We also observed a substantial variation in the sensitivity of CNV detection across different tools, especially in CNVs with a size of less than 1 kb. In general, the breadth of coverage for disease-associated genes and CNVs increased with mean depth. The sensitivity and coverage of WGS (~ 40X) is better than WES (~ 120X). Among the Chinese trios with ~ 40X mean depth, the sensitivity in the offspring was > 99.48% and > 96.36% for SNP and indel detection, and PPVs were 99.86% and 97.93%. All the 12 previously validated variants in the 8 clinical cases were successfully detected by our WGS pipeline.
Conclusions
The current standard of a mean depth of 40X may be sufficient for SNV/indel detection and identification of most CNVs. Clinical scientists should know the range of sensitivity and PPV for different classes of variants for a particular WGS pipeline, which would be useful when interpreting and delivering clinical reports.
Visit-to-Visit Glycemic Variability is Associated with In-Stent Restenosis in Patients with Type 2 Diabetes after Percutaneous Coronary Intervention
Authors: Yang Chen Die , Shen Ying , Lu Lin , Ding Feng Hua , Yang Zhen Kun , Hu Jian , Zhang Rui Yan , Shen Wei Feng , Wang Xiao Qun 
Published: May 2020

Abstract
Background:Patients with type 2 diabetes are under substantially higher risk of in-stent restenosis (ISR) after coronary stent implantation. We sought to investigate whether visit-to-visit glycemic variability is a potential predictor of ISR in diabetic patients after stent implantation.Methods:Type 2 diabetic patients underwent elective percutaneous coronary intervention were consecutively enrolled and 1-year follow-up coronary angiography was performed. The incidence of ISR and its relationship with visit-to-visit HbA1c variability, expressed as coefficient of variation (CV), standard deviation (SD) and variability independent of the mean (VIM), were studied. Multivariable Cox proportional hazards models were constructed to analyze the predictive value of glycemic variability for ISR.Results:From September 2014 to July 2018 in Ruijin Hospital, a total of 420 diabetic patients (688 lesions) after stent implantation were included in the final analysis. During a mean follow-up of 12.8 ± 1.3 months, the incidence of ISR was 8.6%, which was significantly increased in patients with higher CV of HbA1c (P = 0.001). The mean diameter stenosis (DS), net luminal loss and net luminal gain were 22.9 ± 16.8%, 0.42 ± 0.88 mm and 1.66 ± 0.83 mm, respectively. Greater DS was observed in subjects with higher tertiles of CV of HbA1c (P < 0.001), and this trend was more prominent in patients with optimal glycemic control (HbA1c ≤ 7%). In multivariate analysis, HbA1c variability was independently associated with incidence of ISR after adjustment for traditional risk factors and mean HbA1c (HR: 3.00 [95% CI:1.14 ~ 7.92] for highest vs. lowest tertile). Inclusion of CV of HbA1c led to a better risk stratification accuracy. Assessing glycemic variability by SD or VIM yielded similar findings.Conclusions:This study suggests that visit-to-visit HbA1c variability is an independent predictor of incidence of ISR in patients with type 2 diabetes after stent implantation.