bluuebunny/crossref_metadata_2025_split huggingface dataset:
GR, Sgk1, and NDRG1 in esophageal squamous cell carcinoma: their correlation with therapeutic outcome of neoadjuvant chemotherapy
Authors: Ueki Shunsuke , Fujishima Fumiyoshi , Konno Takuro , Ishida Hirotaka , Okamoto Hiroshi , Takaya Kai , Sato Chiaki , Taniyama Yusuke , Kamei Takashi , Sasano Hironobu 
Published: January 2020

Abstract
Background
Esophageal squamous cell carcinoma (ESCC) is a highly malignant neoplasm. Glucocorticoid(GC)-Glucocorticoid receptor (GR) pathway plays pivotal roles in cellular response to various stresses of tumor cells including chemotherapy. However, the status of GC-GR pathway in ESCC, including its correlation with chemotherapeutic responses has remained largely unknown.
Method
GR, serum-and glucocorticoid-regulated kinase 1(Sgk1), and N-myc down regulation gene 1(NDRG1) were immunolocalized in 98 ESCC patients who had undergone esophagectomy following neoadjuvant chemotherapy(NAC) with 2 courses of 5-Fluorouracil(5-FU) + Cisplatin (CDDP). We also examined biopsy specimens before NAC in 42 cases and compared the results between those before and after NAC.
Results
Overall survival (OS) of the patients treated with surgery following NAC was significantly shorter in the group with high GR than that with low GR ( P = 0.0473). Both OS and disease-free survival (DFS) were significantly shorter in both Sgk1- and NDRG1-high groups than low groups (OS: Sgk1, P = 0.0055; NDRG1, P = 0.0021; DFS: Sgk1, P = 0.0240; NDRG1, P = 0.0086). When evaluating the findings in biopsy specimens before NAC, DFS was significantly shorter in the high Sgk1 group ( P = 0.0095), and both OS and DFS was shorter in high NDRG1 group (OS, P = 0.0233; DFS, P = 0.0006) than respective low groups. Among high NDRG1 group of biopsy specimens before NAC, the tumor reduction rate by NAC was significantly attenuated ( P = 0.021).
Conclusions
High status of GR, Sgk1, and NDRG1 in ESCC after NAC was significantly associated with over all worse prognosis and there were no significant changes in the status of those above before and after NAC. Therefore , increased activity of GC-GR pathway with enhanced induction of Sgk1 and NDRG1 in carcinoma cells plays pivotal roles in tumor progression and development of chemoresistance in ESCC patients undergoing NAC.
In Vitro Biomechanical Testing of Equine Metacarpophalangeal Collateral Ligaments and Collateral Ligaments Repair Using Suture Anchors.
Authors: Grzeskowiak Remigiusz Maciej , Rifkin Rebecca E. , Mulon Pierre Yves , Harper David P. , Adair H. Steve , Anderson David E. 
Published: January 2020

Abstract

Background The disruption of equine metacarpophalangeal (MCPCL) and metatarsophalangeal collateral ligament (MTPCL) occurs as a result of traumatic events and it has been associated with guarded to poor prognosis for the return to athletic performance before the injury. Rupture of the MCPCL induces joint laxity which can, directly and indirectly, lead to a cartilage damage of the metacarpophalangeal joint (MCPJ). The literature describes several methods of the surgical management of those injuries, including the suture anchors. However, the knowledge about their biomechanical performance in the equine MCPCL repair model is lacking. This study describes the method of suture anchor placement within the metacarpal bone as well as reveals data of the biomechanical testing. The first objective was to perform bending tests on equine metacarpophalangeal joints with intact metacarpophalangeal collateral ligaments. The second objective was to perform similar tests on the metacarpophalangeal joints, having completely severed metacarpophalangeal collateral ligaments which were reattached to the suture anchors. Results The results of the biomechanical testing of 50 specimens showed that the suture anchor repairs obtained 15% of the intact metacarpophalangeal collateral ligaments’ maximum load, 34% of their bending stiffness, 14% of their load at maximum flexure extension, and 82% of the maximal flexure extension. The suture anchor placement within the bone can be performed quick and in the feasible way. The results of the biomechanical testing did not vary significantly between the tested types of the suture anchors used in this study. Conclusions The repairs with the suture anchors achieved lower biomechanical testing results compared with the intact metacarpophalangeal collateral ligaments; however, the anatomical position of the joint could be easily restored with the repair. The use of anchors may anatomically restore the ligament and provide additional mechanical support for the injured metacarpophalangeal collateral ligaments.
Standardised neonatal parenteral nutrition formulations – Australasian Neonatal Parenteral Nutrition Consensus update 2017
Authors: Bolisetty Srinivas , Osborn David , Schindler Tim , Sinn John , Deshpande Girish , Wong Chee Sing , Jacobs Susan , Phad Nilkant , Pharande Pramod , Tobiansky Rodney , Luig Melissa , Trivedi Amit , McIntosh Joanne , Jozsa Eszter , Opie Gillian , Downe Lyn , Anderson Chad , Bhatia Vineesh , Kumar Prasanna , Malinen Katri , Birch Pita , Simmer Karen , McLeod Gemma , Suzanne Quader , Rajadurai Victor Samuel , Hewson Michael , Nair Arun , Williams Megan , Xiao Jing , Ravindranathan Hari , Broadbent Roland , Lui Kei 
Published: January 2020

Abstract
Background: The first consensus standardised neonatal parenteral nutrition formulations were implemented in many neonatal units in Australia in 2012. The current update involving 49 units from Australia, New Zealand, Singapore, Malaysia and India was conducted between September 2015 and December 2017 with the aim to review and update the 2012 formulations and guidelines. Methods: A systematic review of available evidence for each parenteral nutrient was undertaken and new standardised formulations and guidelines were developed. Results: Five existing preterm Amino acid-Dextrose formulations have been modified and two new concentrated Amino acid-Dextrose formulations added to optimise amino acid and nutrient intake according to gestation. Organic phosphate has replaced inorganic phosphate allowing for an increase in calcium and phosphate content, and acetate reduced. Lipid emulsions are unchanged, with both SMOFlipid (Fresenius Kabi, Australia) and ClinOleic (Baxter Healthcare, Australia) preparations included. The physicochemical compatibility and stability of all formulations have been tested and confirmed. Guidelines to standardise the parenteral nutrition clinical practice across facilities have also been developed. Conclusions: The 2017 PN formulations and guidelines developed by the 2017 Neonatal Parenteral Nutrition Consensus Group offer concise and practical instructions to clinicians on how to implement current and up-to-date evidence based PN to the NICU population.
Reanalysis of the patients who were diagnosed with hystero-epilepsy by Charcot
Authors: Komagamine Tomoko , Kokubun Norito , Hirata Koichi 
Published: January 2020

Abstract
Background: The term “hysteria” is now recognized to refer to non-organic and functional disorders. Jean-Martin Charcot once attempted to identify the lesions responsible for hysteria as an organic disorder in his later years, but his effort failed. He classified hysteria as usual and grand. Grand hysteria was also called hystero-epilepsy, which became obsolete after Charcot’s death and has been an enigma in modern neurology. After anti-NMDA receptor (NMDAR) encephalitis was established in the twenty-first century, a paradigm shift emerged in the interpretation of acute onset of neuropsychiatric symptoms. Patients with anti-NMDAR encephalitis demonstrate a characteristic multistage progression and stereotypical movements, including opisthotonic posture, which are analogous to the staging progressions and ‘ arc de cercle’ observed in Charcot’s hystero-epilepsy. Methods: To elucidate the full picture of Charcot’s hystero-epilepsy, we reviewed the patients presented in “the Tuesday Lessons”.Results: Eleven patients were shown to have hystero-epilepsy by Charcot himself. Of the 11 patients, 4 patients had acute-onset neuropsychiatric symptoms, and 2 patients had a condition that resembled anti-NMDAR encephalitis. Although the clinical courses were similar to those of anti-NMDAR encephalitis, we could not make conclusive diagnoses of anti-NMDAR encephalitis without examinations due to the lack of evidence of teratomas. One patient with alcohol withdrawal syndrome and one patient with chronic pain and morphine withdrawal syndrome were also classified as having hystero-epilepsy by Charcot. The other patients, who manifested chronic courses or varying hypnosis-induced neurological symptoms, could be diagnosed with conversion and dissociative disorders today.Conclusions: Charcot’s hystero-epilepsy has been suspected of being a syndrome encompassing conversion and dissociative disorders, drug withdrawal syndrome, and anti-NMDAR encephalitis-like manifestations. Charcot’s observations combined with the current progress in molecular biology suggest that a common dysfunctional basis exists, such as glutamate/NMDAR system dysfunction, among these conditions.
Anticoccidial effect of “Shi Ying Zi” Powder in chickens infected with Eimeria tenella
Authors: Song Xu , Li Yunhe , Chen Shufan , Jia Renyong , Huang Yongyuan , Yong Ting , Zou Yuanfeng , Li Lixia , Zhao Xinxin , Yin Zhongqiong 
Published: January 2020

Abstract
Background: Coccidiosis is one of the most economically important diseases affecting the poultry industry. Currently, anticoccidial drugs used in veterinary clinical showed many deficiencies, and new control measures are urgently needed. Many plant-derived drugs, such as herbal extracts, showed effects with less drug residues and resistance. Development of herbal medicine as the anticoccidial drug is one of the most prospective method for treating coccidiosis.Methods:“Shi Ying Zi” is the herbal powder that consisted of Cnidium monnieri (L.) Cuss, Taraxacum mongolicum Hand-Mazz and salt with the proportion of 55: 40: 5 (g/g/g). 200 1-day-old broiler chicks free of coccidiosis were equally divided into 10 groups: normal group, untreated group, 4 protective groups (positive control group and three “Shi Ying Zi” powder groups) and 4 therapeutical groups (positive control group and three “Shi Ying Zi” powder groups). At 11 th day of age, the chicks in the four protective groups were prophylactically supplemented with “Shi Ying Zi” powder at the doses of 5g/kg, 10g/kg and 15g/kg and monensin (100mg/kg), respectively. At 14 th day of age, chicks except those in blank group were inoculated with 2×10 4 sporulated oocysts of E. tenella . When the first hemorrhagic fecal was found, therapeutical drugs started administration. The low (5g/kg), medium (10g/kg) and high doses (15g/kg) of “Shi Ying Zi” powder were added into the diet, respectively. Sulfachloropyrazine sodium (positive control) was added into water (1g/L). The anti-coccidial index, biochemical parameters and tissue lesions were determined from 4 d post infection to 8 d post infection. Results: Supplement with “Shi Yin Zi” powder for 3d prior to infection or after infection could improve the survival rate and relative growth rate and alleviate the pathological changes in the cecum, liver and kidney. “Shi Yin Zi” powder could recover the contents of glutamic-pyruvic transaminase, creatinine, albumin and triglycerides in serum. The hemorrhage and total number of oocysts in feces were reduced. The anti-coccidial index reaches to 165. The anti-coccidial effects were equal to positive controls (monensin and sulfamlopyrazine). Conclusions: “Shi Ying Zi” powder possesses potent anticoccidial effect and exhibits the potential to control E.tenella infection.
Analysis of the Influencing Factors of the Medical Service Capacity in Key Clinical Specialties at the County Level Based on TOPSIS
Authors: Wang Kaixuan , Guo Jin-Ling , Wang Wei , Zhao Shui-Chang , Li Miao-Jun , Wang Zhi-Long , Sun Ning , Bi Chun-Xiao , Ma Xin-Sheng 
Published: January 2020

Abstract
To acknowledge the medical service capacity of the first batch of county-level key clinical specialties in Henan province, analyze the influencing factors, and make targeted suggestions. TOPSIS method was used to evaluate the medical service capacity comprehensively, and the influencing factors were analyzed by simple linear regression and multiple linear regression. In the multiple linear regression model, the basic conditions, including the total investment funds during the construction period, net usable area of beds and the ratio of bed to nurse, the quality of care—the compliance rate of major clinical diagnosis and pathological diagnosis, and the personnel training—the number of technical promotion trainees, of the first county-level clinical specialties, have a significant influence upon the medical service capacity; while, the actual number of beds, the ratio of doctors and nurses, the proportion of annual expert visits, the number of technical promotion training and sending of learners, percentage of drug expenditure and core journal articles have limited effect. On the basis of the ensured allocation of resources and capital investment, more attention should be paid to personnel training and appropriate technology promotion, and the medical care quality is supposed to be always insisted on, to promote the overall improvement of medical service capacity in county hospitals.
Medication adherence and Survival prospects among hospitalized heart failure patients in a tertiary hospital in Tanzania: A Prospective Cohort Study.
Authors: Pallangyo Pedro , Millinga Jalack , Bhalia Smita , Mkojera Zabella , Misidai Nsajigwa , Swai Happiness J , Hemed Naairah R , Kaijage Alice , Janabi Mohamed 
Published: January 2020

Abstract
Objective : Management of heart failure is complex and multifaceted but adherence to medications remains the cornerstone of preventing avoidable readmissions, premature deaths, and unnecessary healthcare expenses. Despite of evidence-based efficacy on anti-failure drugs, poor adherence is pervasive and remains a significant barrier to improving clinical outcomes in heart failure population. Results : We enrolled 459 patients with established diagnosis of heart failure. The mean age was 46.4 years and participants aged ≤50 years constituted 55.4% of the cohort. There was a female predominance (56.5%), 67.5% resided in urban areas and 74.2% had primary education. Of the 419 participants eligible for assessment of medication adherence, 313 (74.7%) had poor adherence and 106 (25.3%) had good adherence. Possession of a health insurance was found to be the strongest associated factor for adherence (OR 8.7, 95% CI 4.7-16.0, P <0.001). Participants with poor adherence displayed a 70% increased risk for rehospitalization compared to their counterparts with good adherence (RR 1.7, 95% CI 1.2-2.9, p = 0.04). Poor adherence was found to be the strongest predictor of early mortality (HR 2.5, 95% CI 1.3-4.6, p<0.01). Poor medication adherence in patients with heart failure is associated with increased readmissions and mortality.
In vitro methodological standardization of MTT colorimetric assay for evaluation of formazan activity and drug dosage
Authors: Nepomuceno Leandro , Carvalho Luciana Silva de , Soares Nayane Peixoto , Cruz Vanessa de Sousa , Arnhold Emmanuel , Ferreira Jorge Luís , Araújo Eugênio Gonçalves de 
Published: January 2020

Abstract
Background Aiming to develop essential chemotherapeutic drugs to control various types of cancer, one of the primary tests is the tetrazolium reduction method. The present study aims to standardize the electromagnetic spectrum that best discriminates the absorbance and to evaluate the drug dosage of single and daily doses during the cell viability and toxicity analysis by the tetrazolium reduction method, using the ethanol extract of pequi peel in canine osteosarcoma cells. Results The wavelength of 532 nm showed the best results. We found that as the treatment time increased, the formazan conversion reduced. After 72 hours of treatment, we observed clear discrimination of dose-dependent data, with up to five discriminants within 72 hours with a change in absorbance from 0.554 to 0.064 A. The wavelength of 570 nm it was not ideal since we could not discern the difference in the spectral reflectance of the treatments and, therefore, show no statistical difference among treatments. We found no statistical difference for the coefficient of variation at wavelengths of 532 and 570nm, which were 12.77% and 8.80% respectively. Conclusions The wavelength of 532nm best discriminated the absorbance, as it presented better ability to congregate the treatment groups, greater variation between the discriminant and lower coefficient of variation, during the colorimetric test to evaluate the cellular metabolism. Moreover, the ethanol extract of pequi peel in canine osteosarcoma cells showed a statistically equal effect of a single dose administration to the dose reapplied every 24 hours.
Quantitative analysis of left atrial myocardial function and compliance in patients with pre-eclampsia
Authors: Sun Fei , Li Yong , Wang Wugang , Xu Lin , Zhao Min , Li Rong , He Xiangqin , Cong Juan 
Published: January 2020

Abstract
Background: Pre-eclampsia (PE) is a severe obstetric complication associated with many cardiovascular disorders. Left atrial (LA) function and its relevance to the cardiovascular adaptation changes associated with PE have not been adequately addressed. The aim of this study was to quantify LA myocardial deformation characteristics in women with PE using speckle tracking echocardiography (STE). Methods: Using STE, LA global longitudinal strain (LAGLS), phasic LA strain values and LASr/(E/e'), which was the value of LA reservoir strain (LASr) divided by the quotient of early mitral flow velocity (E) and diastolic mitral ring velocity (e'), were compared among nonpregnant controls (n=39), normotensive pregnant women (n=40), and PE (n=43) patients. Results: LAGLS, LASr and LA conduit strain reduced in PE group in spite of no difference of LA contraction strain among the three groups. LASr/(E/e'), the surrogate of LA compliance, was decreased during PE pregnancy. The multivariate regression analyses showed LAGLS was independently correlated with LASr/(E/e'), left ventricular global longitudinal strain and LA maximum volume. Conclusion: PE cases undergo impaired LA myocardial function and decreased atrial compliance. STE-derived LA strain may be a powerful diagnostic parameter to evaluate diastolic function with the progression of PE pregnancy.
Endocardial radiofrequency septal ablation for hypertrophic obstructive cardiomyopathy:two case reports
Authors: zhao ruohan , Xiong Feng , Wang Shuzhen , Liu Hanxiong , Luo Duan 
Published: January 2020

Abstract
Endocardial radiofrequency ablation of the septal hypertrophy (ERASH) is an alternative for hypertrophic obstructive cardiomyopathy (HOCM). Radiofrequency ablation could lead to regional myocardial akinesia, so as to relieve the left ventricular outflow tract (LVOT) gradient. We reported that two patients presented Left ventricular asymmetric hypertrophy in transthoracic echocardiography, and were diagnosed with HOCM. One had a gradient up to 210mmHg during the provocation. The other had a gradient of LVOT up to 108mmHg at rest. Both are symptomatic despite of the optimized medication. Due to the restriction of coronary anatomy, the patients lost the opportunity for the alcohol septal ablation (ASA). ERASH is programmed. The target site was determined by intracardial echocardiography and electroanatomic Carto-mapping system. ERASH caused the targeted myocardium hypokinesia precisely, aiming for interdicting mitral valve systolic anterior motion. 1-month follow-up showed a remarkable improvement of clinical state and a predominant reduction of LVOT gradient assessing by transthoracic echocardiography. ERASH comes up with a new solution for the patient who is not candidate for ventricular septal myectomy and ASA. Carto-mapping system and intracardiac echocardiography enable precise lesion placement and preservation of atrioventricular conduction.
A Scientometric Analysis of Neuroblastoma Research
Authors: Martynov Illya , Klima-Frysch Jessica , Schoenberger Joachim 
Published: January 2020

Abstract
Background: There are thousands of research articles in the field of neuroblastoma that have been published over the past few decades. However, the heterogeneity and variable quality of scholarly data may challenge scientists or clinicians to survey all published articles. However, holistic measuring and analyzing of neuroblastoma related literature by sophisticated mathematical methods can provide a unique opportunity to gain deep insights into the global research performance and collaborative architectonical structure within the neuroblastoma scientific community. In this scientometric study, we aim to determine the extent of the scientific output related to neuroblastoma research, focusing on the time period between 1980 and 2018. Methods: We apply novel scientometric tools, including Bibliometrix R package, biblioshiny, VOSviewer, and CiteSpace IV for comprehensive science mapping analysis of extensive bibliographic metadata which was retrieved from the Web of ScienceTM Core Collection database. Results: We demonstrate the enormous proliferation of neuroblastoma research during last the 38 years, including 12,435 documents published in 1,828 academic journals by 36,908 authors from 86 different countries. We determine the proportion of highly cited and never cited papers, “occasional” and “core” authors and journals. We identify the six most important clusters of authors and their interactions. Further, we show 10 (11.6%) of 86 countries were responsible for the three quarters of NB-related research output. Conclusions: These findings are crucial for researchers, clinicians, journal editors, and consortiums working in neuroblastoma area to understand the strengths and potential gaps in neuroblastoma research and to plan future investments in data collection and science policy. This first scientometric study of global neuroblastoma research performance provides valuable insight into the scientific landscape, co-authorship network architecture, international collaboration, and interaction within the neuroblastoma community.