bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reconstruction of Paprosky type III Acetabular Defects by the Three-dimensional Printed Porous Augment: Techniques and Clinical Outcomes of 18 Consecutive Cases
Authors: Fu Jun , Ni Ming , Li Xiang , Chai Wei , Hao Libo , Zhou Yonggang , Zhang Guoqiang , Chen Jiying 
Published: October 2020

Abstract
Background and Purpose: A major challenge posed by primary and revision total hip arthroplasty (THA) is the management of severe acetabular bone defect. Previous surgical techniques have certain limitations in the anatomical reconstruction and accurate match of severe acetabular defects. Until now, reports are scanty on the clinical outcomes of acetabular reconstruction by the three-dimensional (3D) printed porous augments in bone defect patients. This study reported the clinical outcomes of reconstruction of Paprosky type III acetabular defects by 3D printed porous augments.Methods: 18 patients with Paprosky type III acetabular defects receiving reconstructive surgery by 3D printed porous augments were included in current study. Their data, including general information, intra-operative findings, imaging results, functional scores and complications were retrospectively analyzed.Results: The mean follow-up time lasted 33.3 ± 2.0 (24-56) months. The average limb-length discrepancy (LLD) was 31.7 ± 4.2 (3-59) mm preoperatively, 7.7 ± 1.4 (1-21) mm postoperatively (p<0.0001) and 7.5 ± 1.2 (0-18) mm at the latest follow-up. The mean vertical position of hip center of rotation (HCOR) from the inter teardrop line changed from preoperative 50.7 ± 3.9 (23.3-75.3) mm to postoperative 22.9 ± 1.9 (10.1-40.3) mm (p<0.0001), with the latest follow-up revealing an HCOR of 22.3 ± 1.7 (11.0-40.5) mm. Follow-up study showed that no hip had radiolucencies and radiological loosening of the acetabular components and augment. The average HHS improved from 40.3 ± 4.5 (10.5-71) before operation to 88.4 ± 1.9 (75-97) at the last follow-up (p<0.0001). Moreover, follow-up exhibited that no periprosthetic joint infection, hip dislocation, fracture and re-revision occurred. Conclusion: Surgical treatment of Paprosky type III acetabular defect with 3D printed porous augment was simple, achieved good match between porous augment and the defect bone surface and the acetabular component, ideally restored LLD and HCOR after operation, significantly improved HHS score and attained good early clinical outcomes. It is a promising personalized solution for patients with severe acetabular bone defect.
Septin4 Aggravates Hypoxia-Induced Cardiomyocytes Injury by Promoting HIF-1α Ubiquitination and Degradation through VHL
Authors: Wu Shaojun , Zhang Ying , You Shilong , Lu Sien , Zhang Naijin , Sun Yingxian 
Published: October 2020

Abstract
Backgrounds: Myocardial ischemia, characterized by insufficient nutrients and oxygen supply, is the most common cause of ischemic heart disease. Hypoxia-induced cardiomyocytes injury is the pathogenic feature of myocardial ischemia. Although previous studies have reported that the proapoptotic protein Septin4 contributes to prevent some cancers by promoting tumor cells apoptosis mainly through X-linked inhibitors of apoptosis (XIAPs), little is known its role in hypoxia-induced cardiomyocytes injury and its other new interacting partner. Results: In the current study, Septin4 is found to be involved in cardiomyocytes injury to hypoxia. The overexpression of Septin4 significantly aggravated hypoxic cardiomyocytes apoptosis assessed by cell viability assay and flow cytometry analysis. Mechanistically, hypoxia-inducible factor 1 alpha (HIF-1α) is confirmed as a novel protein mainly binding with GTPase domain of Septin4 by co-immunoprecipitation. Additionally, we found that the protective factor HIF-1α is down-regulated by Septin4 and the underlying mechanism is the von Hippel-Lindau protein (VHL)-mediated ubiquitin-proteasome degradation. Conclusions: These findings suggest that Septin4 aggravates hypoxia-induced cardiomyocytes injury by promoting HIF-1α ubiquitination and degradation by targeting to VHL, which may be beneficial to provide effective strategies for clinical treatment of myocardial ischemia and ischemic heart disease.
Genomic Variations in SARS-CoV-2 Strains at the Target Sequences of Nucleic Acid Amplification Tests
Authors: Cao Canhui , Yu Ruidi , Zeng Shaoqing , Liu Dan , Gong Wenjian , Li Ruyuan , Wang Siyuan , Yuan Yuan , Chi Jianhua , Liu Jiahao , Yu Yang , Jiao Xiaofei , Cai Guangyao , Jin Ning , Ye Fei , Gao Qinglei 
Published: October 2020

Abstract
Background: Nucleic acid amplification is the main method used to detect infections of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). However, the false-negative rate of nucleic acid tests cannot be ignored. Methods: Herein, we demonstrated genomic variations at the target sequences for the tests and the geographical distribution of the variations across countries by analyzing the whole-genome sequencing data of SARS-CoV-2 strains from the 2019 Novel Coronavirus Resource (2019nCoVR) database. Results: Among the 21 pairs of primer sequences in regions ORF1ab, S, E, and N, the total length of primer and probe target sequences was 938bp, with 131(13.97%) variant loci in 2415 (38.96%) isolates. Primer targets in the N region contained the most variations that were distributed among the most isolates, and the E region contained the least. Single nucleotide polymorphisms were the most frequent variation, with C to T transitions being detected in the most variant loci. G to A transitions and G to C transversions were the most common and had the highest isolate density. Genomic variations at the three mutation sites N: 28881, N: 28882, and N: 28883 were the most commonly detected, including in 608 SARS-CoV-2 strains from 33 countries, especially in the United Kingdom, Portugal, and Belgium. Conclusions: Our work comprehensively analyzed genomic variations on the target sequences of the nucleic acid amplification tests, offering evidence to optimize primer and probe target sequence selection, thereby improving the performance of the SARS-CoV-2 diagnostic test.
Chenopodium album is a weed host of Meloidogyne incognita (Nematoda: Meloidogynidae) in Peru
Authors: Gómez-Chatata Jorge Airton , Casa-Ruiz Teodocia Gloria , Tamo-Zegarra Juan José , Bellé Cristiano 
Published: October 2020

Abstract

Chenopodium album plants showing symptoms caused by root-knot nematodes were detected in the La Joya, Arequipa, Peru. Based on the morphological, esterase phenotypes, and molecular analyses of the mitochondrial DNA region between the cytochome oxidase subunit II and 16S rRNA genes (mtDNA) and species-specific characterized amplified region, the causal agent of the observed symptoms was identified as Meloidogyne incognita. Pathogenicity was confirmed by fulfilling a modified version of Koch’s postulates. To our knowledge, this is the first report of M. incognita parasitizing C. album in Peru.
Derechos Humanos como factor para la humanización de la ciencia jurídica.
Authors: Villalba Bernié Pablo Darío 
Published: October 2020

El artículo de reflexión abordado destaca la transformación de las ciencias jurídicas que en las últimas décadas ha sido irradiado por el factor de humanización del derecho, que indica el derrotero a seguir en pos de la modernidad. En un avance gradual, pero avasallante, se han venido imponiendo los criterios hermenéuticos a la luz de las doctrinas de los derechos humanos, que conducen a una mutación significativa de la juridicidad universal. En América Latina el impacto es aún mayor como resultado de la incidencia del SistemaInteramericano de Derechos Humanos y de la doctrina del control de convencionalidad, marcando un novel sendero al ámbito jurídico. La idea de este trabajo es apuntalar los hitos orientadores que deberán analizarse para comprender las razones, con el fin de generar elrescate del ser humano como centro de la protección jurídica.Palabras clavesDerechos humanos, humanización, ius filosofía, modernidad jurídica, paradigmas.
Pluralismo jurídico en el departamento del Putumayo.
Authors: Saidiza Peñuela Juan Pablo 
Published: October 2020

Se presenta un análisis sobre el pluralismo jurídico en el departamento del Putumayo a partir de nuevas postulaciones teóricas basadas en la crisis del derecho moderno y su incapacidad para resolver la totalidad de los litigios. Este escrito está sustentado en un estudio de caso con el pueblo indígena murui muina, con quienes se ha establecido una relación de trabajo e investigación para fortalecer las formas de administración de justicia en espacios locales, que impliquen una mirada constructiva más apropiada en la construcción de la autonomía de los pueblos indígenas en el sur del país.
Palabras clave:Pluralismo jurídico, Justicia propia indígena, Barreras de acceso a la justicia, Interculturalidad, Norma social.
Política Editorial
Authors: Espinel Torres Yeilor Rafael , Camelo Zamudio Jenny Astrid , Silva Serna Juan Sebastián , Molina Orjuela Douglas Eduardo , Rojas Alonso Valeria , Reyes Guzmán Sebastián Camilo 
Published: October 2020

La Revista Criterios es un proyecto humano pensado para nacer y madurar de acuerdo con el ritmo cambiante de los tiempos. Aspiramos a que nuevas plumas envíen sus disertaciones, que nos critiquen, que propongan, que vean en nuestras páginas la posibilidad de un dialogo académico genuino y de gran altura.
Ferulic Acid Activity in Topical Formulations: Technological and Scientific Prospecting
Authors: Cavalcanti Gabriela R. , Duarte Fernanda I.C. , Converti Attilio , de Lima Ádley A.N. 
Published: October 2020

Introduction:
Ferulic acid is a phenolic compound widely distributed in monocotyledons, with several
applications, especially in pharmaceutical and dermo-cosmetic industries. It has proven antioxidant and anti-inflammatory
activities, among others, which are mainly ascribed to its molecular structure. The main factor that can lead to serious skin
damages like inflammation, dryness, wrinkles, and cancer is the exposure to UV radiation that is responsible for an increased
level of radical oxygen species.

Objective:
This review aims to evaluate the application of ferulic acid in topical formulations and the technologies used to
enhance its bioavailability and stability, as well as to get a clearer picture of its effects by in vivo and in vitro studies.

Methods:
It covers technological publications in the WIPO, EPO, INPI, and USPTO databases and scientific publications
in the PubMed, Web of Sciences, and Science Direct databases, exploring the trend and application of this compound by
country and year of publication.

Results:
Both the scientific and technological analysis showed the importance and tendency in the association of the Ferulic
Acid and other vitamins and actives. The synergic effect certainly provides a better result, performance and stability of the
compounds. Witch cleared the great spectrum and applicability of the Ferulic Acid in topical formulations.

Conclusion:
The present literature survey revealed that ferulic acid exerts an important activity in several formulations for
topical application and improved stability and bioavailability when combined with new technologies and methods, showing
an open path to target the treatment of skin disorders.
Exploring the Mechanism of Swertia mussotii Franch. for Hepatoprotective Effects with iTRAQ LC-MS/MS
Authors: Yun Haixia , Wu Xinyu , Ding Yiwei , Xiong Wendou , Duan Xianglan , Gao Hui , Yang Yingzhong , Chen Zhi 
Published: October 2020

Background and Objective:
A Tibetan traditional herb named Swertia mussotii Franch.,
also called “Zangyinchen” by the local people of the Qinghai-Tibet area, has been used to protect
the liver from injury for many years. However, the curative effect and molecular mechanism of the
herb have not been demonstrated clearly.

Materials and Methods:
In our study, serum alanine aminotransferase, aspartate aminotransferase,
and total bilirubin levels were examined after S. mussotii Franch. treatment of the acute liver injury
on the carbon tetrachloride-induced rat model. Then, proteome analysis was conducted to explore
the potential mechanism of SMT for hepatoprotective effects after iTRAQLC-MS/MS analysis
(isobaric tag for relative and absolute quantification-liquid chromatography-mass spectrometer
with tandem mass spectrometry).

Results:
Serum results showed that alanine aminotransferase, aspartate aminotransferase, and total
bilirubin levels of rats with acute liver injury were all improved with SMT treatment. Moreover,
proteome analysis suggested that, with S. mussotii Franch. treatment, the levels of lipid catabolic
process and lipid homeostasis were all enhanced. Furthermore, the results of protein-protein
interaction (PPI) analysis illustrated that the proteins assembled in PPI networks were found to be
significantly enriched in response to lipid, negative regulation of lipase activity, and in response to
lipopolysaccharides. Furthermore, the down-regulated MRP14 and MRP8 proteins were found to
be involved in the lipid metabolism, which may indicate the mechanism of SMT for the protection
of the liver from ALI induced by carbon tetrachloride.

Conclusion:
SMT herb could play a role in hepatoprotection and alleviating the acute liver injury
by impacting the lipid metabolism associated with the biological process.
The Influence of Diabetes Mellitus on Rotator Cuff Repair: A Systematic Review and Meta-Analysis
Authors: Lu Xiaomin , Sun He , Xu YiSheng , Cao Xuewei 
Published: October 2020

Background:
Clinical outcomes after rotator cuff repair associated with diabetes
mellitus (DM) are generally favorable, but no study has attempted to establish the influence of DM
on outcomes after rotator cuff repair.

Purpose:
To conduct a meta-analysis of clinical studies evaluating patient outcomes between
people with DM and people without DM after rotator cuff repair.

Study Design:
Meta-analysis.

Methods:
A literature search of the Embase, PubMed, and Cochrane Library databases was
performed according to the PRISMA (Preferred Reporting Items for Systematic Reviews and
Meta-Analyses) guidelines. Cohort studies and case-control studies about clinical outcomes after
rotator cuff repair comparing people with DM and people without DM were included. Statistical
analysis was performed with RevMan (v 5.3.3).

Results:
Nine clinical studies that met the inclusion criteria were identified and included a total of
314 patients treated with DM and 1092 patients without DM. The failure rate was significantly
higher in the DM group than in Non-DM group (23.97% compared with 16.60%, OR: 2.39; 95%
CI, 1.69–3.37; p < 0.001). The postoperative retear rate and showed a significant difference
between the two groups (24.5% and 13.7%; OR: 2.41; 95% CI, 1.57–3.71; p<0.001). The DM
group showed a higher rate of postoperative unhealed cuff than the Non-DM group(41.81% and
25.23%; OR: 2.14; 95% CI, 1.69–3.37; p=0.01).Postoperative Range of motion(ROM) at 12
months after surgery show a significant difference in the range of external rotation between two
groups (WMD: -6.02; 95% CI, -7.54 to -4.50; p<0.001). The preoperative Japanese Orthopaedic
Association (JOA) score, the comparison of pre- and post-operative JOA scores showed a
significant difference in the DM and Non-DM group(p<0.001). The postoperative JOA score, the
pre- and post-operative muscle strength, the pre- and post-operative visual analog scale (VAS)
show significant difference between the DM and Non-DM group(p<0.001).

The postoperative infection rates, the rates of postoperative shoulder stiffness, the preoperative
ROM, the postoperative ROM at 6 months, the postoperative ROM at 12 months of forward
flexion and abduction, the American Shoulder and Elbow Surgeons score, the University of
California, Los Angeles scores, and the preoperative Constant-Murley scores show no significant
difference between the two groups.

Conclusion:
This meta-analysis indicates that DM may be relative to a higher rate of shoulder
retear and cuff unhealing. However, patients with DM can achieve great clinical outcomes after
cuff repair, compared to patients without DM.
Antibacterial Potential of a Novel Peptide from the Consensus Sequence of Dermaseptin Related Peptides Secreted by Agalychnis annae
Authors: Ajingi Ya’u Sabo , Muhammad Auwal , Khunrae Pongsak , Rattanarojpong Triwit , Pattanapanyasat Kovit , Sutthibutpong Thana , Jongruja Nujarin 
Published: October 2020

Background:The consistently increasing reports of bacterial resistance and the reemergence of bacterial epidemics have inspired the health and scientific community to discover new molecules with antibacterial potential continuously. Frog-skin secretions constitute bioactive compounds essential for finding new biopharmaceuticals. The exact antibacterial characterization of dermaseptin related peptides derived from Agalychnis annae, is limited. The resemblance in their conserved and functionally linked genomes indicates an unprecedented opportunity to obtain novel bioactive compounds.Objective:In this study, we derived a novel peptide sequence and determined its antibacterial potentials.Method:Consensus sequence strategy was used to design the novel and active antibacterial peptide named 'AGAAN' from skin secretions of Agalychnis annae. The In-vitro activities of the novel peptide against some bacterial strains were investigated. Time kill studies, DNA retardation, cytotoxicity, beta-galactosidase, and molecular computational studies were conducted.Results:AGAAN inhibited P. aeruginosa, E. faecalis, and S. typhimurium at 20 µM concentration. E. coli and S. aureus were inhibited at 25 µM, and lastly, B. subtilis at 50 µM. Kinetics of inactivation against exponential and stationary growing bacteria was found to be rapid within 1-5 hours of peptide exposure, depending on time and concentration. The peptide displayed weak hemolytic activity between 0.01%–7.31% at the antibacterial concentrations. AGAAN efficiently induced bacterial membrane damage with subsequent cell lysis. The peptide's DNA binding shows that it also targets intracellular DNA by retarding its movement. Our in-silico molecular docking analysis displayed a strong affinity to the bacterial cytoplasmic membrane.Conclusion:AGAAN exhibits potential antibacterial properties that could be used to combat bacterial resistance.
Biosynthesis of Silver Nanoparticles from Citrobacter freundii as Antibiofilm Agents with their Cytotoxic Effects on Human Cells
Authors: Shakhatreh Muhamad A.K. , Al-Rawi Omar F. , Swedan Samer F. , Alzoubi Karem H. , Khabour Omar F. , Al-Fandi Mohamed 
Published: October 2020

Background:
Nanomaterials have recently been identified for their potential benefits in the areas of medicine
and pharmaceuticals. Among these nanomaterials, silver nanoparticles (Ag-NPs) have been widely utilized in the fields of
diagnostics, antimicrobials, and catalysis.

Objective:
To investigate the potential utility of Citrobacter freundii in the synthesis of silver nanoparticles (Ag-NPs), and
to determine the antimicrobial activities of the Ag-NPs produced.

Methods:
Aqueous Ag+ ions were reduced when exposed to C. freundii extract and sunlight, leading to formation of AgNPs. Qualitative microanalysis for the synthesized Ag-NPs was done using UV-vis spectrometry, energy dispersive X-ray
analysis (EDX), and scanning and transmission electron microscopy. The hydrodynamic size and stability of the particles
were detected using dynamic light scattering (DLS) analysis. The Ag-NPs’ anti-planktonic and anti-biofilm activities against
Staphylococcus aureus and Pseudomonas aeruginosa, which are two important skin and wound pathogens, were investigated. The cytotoxicity on human dermal fibroblast cell line was also determined.

Results:
Ag-NPs were spherical with a size range between 15 to 30 nm. Furthermore, Ag-NPs displayed potent bactericidal
activities against both S. aureus and P. aeruginosa and showed noticeable anti-biofilm activity against S. aureus biofilms.
Ag-NPs induced minor cytotoxic effects on human cells as indicated by a reduction of cell viability, a disruption of plasma
membrane integrity, and apoptosis induction.

Conclusion:
Ag-NPs generated in this study might be a future potential alternative to be used as antimicrobial agents in
pharmaceutical applications for wound and skin related infections.
A New Series of Triazolothiadiazines as Potential Anticancer Agents for Targeted Therapy of Non-Small Cell Lung and Colorectal Cancers: Design, Synthesis, In silico and In vitro Studies Providing Mechanistic Insight into Their Anticancer Potencies
Authors: Sever Belgin , Altıntop Mehlika Dilek , Çiftçi Gülşen Akalın , Özdemir Ahmet 
Published: October 2020

Background:
Targeted therapies acting on specific molecular targets in cancer cells with
better curative efficacy and lower toxicity have come into prominence for the management of nonsmall
cell lung cancer (NSCLC) and colorectal cancer (CRC). COX-2 stands out as a plausible target
for anticancer agents due to its pivotal role in tumor initiation, progression and invasion.

Objectives:
Due to the importance of triazolothiadiazine scaffold in targeted anticancer drug discovery,
the aim of this work is the design of new triazolothiadiazines as potential anticancer agents for
the targeted therapy of NSCLC and CRC.

Methods:
New triazolo[3,4-b]-1,3,4-thiadiazines (2a-g) were synthesized via the ring closure reactions
of 2-bromo-1-arylethanones with 4-amino-5-((5-methoxy-2-methyl-1H-indol-3-yl)methyl)-2,4-
dihydro-3H-1,2,4-triazole-3-thione (1), which was obtained via the solvent-free reaction of 5-
methoxy-2-methyl-3-indoleacetic acid with thiocarbohydrazide. MTT assay was performed to determine
their cytotoxic effects on A549 human lung adenocarcinoma, Caco-2 human colorectal adenocarcinoma
and CCD-19Lu human lung fibroblast cells. The most potent compounds were evaluated
for their effects on apoptosis, caspase-3, mitochondrial membrane potential, cell cycle, ultrastructural
morphological changes and COX-2 in A549 and Caco-2 cells. In silico docking and Absorption, Distribution,
Metabolism and Excretion (ADME) studies were also performed using Schrödinger’s
Maestro molecular modeling package.

Results:
6-(4-Chlorophenyl)-3-[(5-methoxy-2-methyl-1H-indol-3-yl)methyl]-7H-[1,2,4]triazolo[3,4-
b][1,3,4]thiadiazine (2e) was the most potent and selective anticancer agent in this series against
A549 and Caco-2 cell lines. Compound 2e induced early apoptosis, caused mitochondrial membrane
depolarization and arrested cell cycle at G0/G1 phase in A549 cells. On the other hand, compound 2e
triggered intrinsic apoptotic pathway involving caspase-3 activation in Caco-2 cells. Compound 2e
caused apoptotic morphological changes in both cancer cell lines. The cytotoxic and apoptotic effects
of this compound on CRC were found to be related to its selective COX-2 inhibitory activity. According
to molecular docking studies, compound 2e showed good affinity to the active site of COX-2
(PDB code: 4COX). Based on in silico ADME studies, the compound is predicted to possess a favorable
ADME profile.

Conclusion:
According to in vitro and in silico studies, compound 2e was identified as a potential
orally bioavailable anticancer agent for COX-2-targeted therapy of CRC.