bluuebunny/crossref_metadata_2025_split huggingface dataset:
Implications for tetraspanin-enriched microdomain assembly based on structures of CD9 with EWI-F
Authors: Oosterheert Wout , Xenaki Katerina T. , Neviani Viviana , Pos Wouter , Doulkeridou Sofia , Manshande Jip , Pearce Nicholas M. , Kroon-Batenburg Loes M. J. , Lutz Martin , van Bergen en Henegouwen Paul M. P. , Gros Piet 
Published: June 2020

AbstractTetraspanins are ubiquitous eukaryotic membrane proteins that contribute to a variety of signaling processes by spatially organizing partner-receptor molecules in the plasma membrane. How tetraspanins bind and cluster partner receptors into so-called tetraspanin-enriched microdomains is unknown. Here we present crystal structures of the large extracellular loop of CD9 in complex with nanobodies 4C8 and 4E8; and, the cryo-EM structure of 4C8-bound CD9 in complex with its prototypical partner EWI-F. The CD9 - EWI-F complex displays a tetrameric arrangement with two centrally positioned EWI-F molecules, dimerized through their ectodomains, and two CD9 molecules, one bound to each EWI-F single-pass transmembrane helix through CD9-helices h3 and h4. In the crystal structures, nanobodies 4C8 and 4E8 bind CD9 at the C and D loop, in agreement with 4C8 binding at the ends of the CD9 - EWI-F cryo-EM complex. Overall, the 4C8 - CD9 - EWI-F - EWI-F - CD9 - 4C8 complexes varied from nearly two-fold symmetric (i.e. with the two CD9 - 4C8 copies in nearly anti-parallel orientation) to ca. 50° bent arrangements. Since membrane helices h1 and h2 and the EC2 D-loop have been previously identified as sites for tetraspanin homo-dimerization, the observed linear but flexible arrangement of CD9 - EWI-F with potential CD9 - CD9 homo-dimerization at either end provides a new ‘concatenation model’ for forming short linear or circular assemblies, which may explain the occurrence of tetraspanin-enriched microdomains.
An alpaca nanobody neutralizes SARS-CoV-2 by blocking receptor interaction
Authors: Hanke Leo , Perez Laura Vidakovics , Sheward Daniel J. , Das Hrishikesh , Schulte Tim , Moliner-Morro Ainhoa , Corcoran Martin , Achour Adnane , Hedestam Gunilla B. Karlsson , Hällberg B. Martin , Murrell Ben , McInerney Gerald M. 
Published: June 2020

AbstractSARS-CoV-2 is the etiologic agent of COVID-19, currently causing a devastating pandemic for which pharmacological interventions are urgently needed. The virus enters host cells through an interaction between the spike glycoprotein and the angiotensin converting enzyme 2 (ACE2) receptor. Directly preventing this interaction presents an attractive possibility for suppressing SARS-CoV-2 replication. Here we report the isolation and characterization of an alpaca-derived single domain antibody fragment, Ty1, that specifically targets the receptor binding domain (RBD) of the SARS-CoV-2 spike, directly preventing ACE2 engagement. The nanobody binds with high affinity in the low nM range to the RBD, occluding ACE2. A cryo-electron microscopy structure of the bound complex at 2.9 Å resolution reveals that Ty1 binds to an epitope on the RBD accessible in both the ‘up’ and ‘down’ conformations and that Ty1 sterically hinders RBD-ACE2 binding. This 12.8 kDa nanobody does not need an Fc domain to neutralize SARS-CoV-2, and can be expressed in high quantities in bacteria, presenting opportunities for manufacturing at scale. Ty1 is therefore an excellent candidate as an intervention against COVID-19.
A Rab Escort Protein Regulates the MAPK Pathway That Controls Filamentous Growth in Yeast
Authors: Jamalzadeh Sheida , Cullen Paul J. 
Published: June 2020

ABSTRACTMAPK pathways regulate different responses yet can share a subset of common components. In this study, a genome-wide screen was performed to identify genes that, when overexpressed, induce a growth reporter (FUS1-HIS3) that responds to ERK-type MAPK pathways (Mating/filamentous growth or fMAPK) but not p38-type MAPK pathways (HOG) in yeast. Approximately 4,500 plasmids overexpressing individual yeast genes were introduced into strains containing theFUS1-HIS3reporter by high-throughput transformation. Candidate genes were identified by measuring the degree of growth, which was a reflection of reporter activity. Of fourteen genes identified and validated by re-testing, two were metabolic controls (HIS3andATR1), five had established roles in regulating ERK-type pathways (STE4, STE7, BMH1, BMH2, MIG2) and seven represent potentially new regulators of MAPK signaling (RRN6, CIN5, MRS6, KAR2, TFA1, RSC3, RGT2).MRS6encodes a Rab escort protein and effector of the TOR pathway that plays an established role in nutrient signaling.MRS6overexpression stimulated filamentous/invasive growth and phosphorylation of the ERK-type fMAPK, Kss1. Overexpression ofMRS6reduced the osmotolerance of cells and phosphorylation of the p38/HOG pathway MAPK, Hog1. Mrs6 interacted with the PAK kinase Ste20 and MAPKK Ste7 by two-hybrid analysis. Collectively, the data indicate that Mrs6 may function to selectively propagate an ERK-dependent signal. Generally speaking, the identification of new MAPK pathway regulators by genetic screening in yeast may be a useful resource for understanding signaling pathway regulation.
STAT3 Serine phosphorylation is required for TLR4 metabolic reprogramming and IL-1β expression
Authors: Balic Jesse , Albargy Hassan , Luu Kevin , Kirby Francis J , Jayasekara W. Samantha N. , Mansell Finbar , Gamara Daniel J , De Nardo Dominic , Baschuk Nikola , Louis Cynthia , Humphries Fiachra , Fitzgerald Katherine , Latz Eicke , Gough Daniel J , Mansell Ashley 
Published: June 2020

ABSTRACTDetection of microbial components such as lipopolysaccharide (LPS) by Toll-like receptor (TLR)-4 expressed on macrophages induces a robust pro-inflammatory response which has recently been shown to be dependent on metabolic reprogramming1, 2, 3, 4. These innate metabolic changes have been compared to the Warburg effect (also known as aerobic glycolysis) described in tumour cells5, 6. However, the mechanisms by which TLR4 activation leads to mitochondrial and glycolytic reprogramming remain unknown. Here we show that TLR4 activation induces a signalling cascade recruiting TRAF6 and TBK-1, while TBK-1 phosphorylates STAT3 on S727. Using a genetically engineered mouse model incapable of undergoing STAT3 Ser727 phosphorylation, we show bothex vivoandin vivothat STAT3 Ser727 phosphorylation is critical for LPS-induced glycolytic reprogramming, the production of the central immune-metabolite succinate and inflammatory cytokine production in a model of LPS-induced inflammation. Our study identifies non-canonical STAT3 activation as the crucial signalling intermediary for TLR4-induced glycolysis, macrophage metabolic reprogramming and inflammation.
How antiporters exchange substrates across the cell membrane? An atomic-level description of the complete exchange cycle in NarK
Authors: Feng Jiangyan , Selvam Balaji , Shukla Diwakar 
Published: June 2020

AbstractMajor facilitator superfamily (MFS) proteins operate via three different mechanisms: uniport, symport, and antiport. Despite extensive investigations, molecular understanding of antiporters is less advanced than other transporters due to the complex coupling between two substrates and the lack of distinct structures. We employ extensive (~300 μs) all-atom molecular dynamics simulations to dissect the complete substrate exchange cycle of the bacterial antiporter, NarK. We show that paired basic residues in the binding site prevent the closure of unbound protein and ensure the exchange of two substrates. Conformational transition only occurs in the presence of substrate, which weakens the electrostatic repulsion and stabilizes the transporter by ~1.5 ± 0.1 kcal/mol. Furthermore, we propose a state-dependent substrate exchange model, in which the relative spacing between the paired basic residues determines whether and bind simultaneously or sequentially. Overall, this work presents a general working model for the antiport mechanism within MFS family.
Experimental study of wake steadiness of an airfoil in pitch–hold–return motion
Authors: Masdari Mehran , Ghorbani Maryam , Tabrizian Arshia 
Published: June 2020

Purpose
The purpose of this paper is to analyze experimentally subsonic wake of a supercritical airfoil undergoing a pitch–hold–return motion. The focus of the investigation has been narrowed to concentrate on the steadiness of the flow field in the wake of the airfoil and the role of reduced frequency, amplitude and the hold phase duration.

Design/methodology/approach
All experiments were conducted in a low sub-sonic closed-circuit wind tunnel, at a Reynolds number of approximately 600,000. The model was a supercritical airfoil having 10% thickness and wall-to-wall in ground test facilities. To calculate the velocity distribution in the wake of the airfoil, total and static pressures were recorded at a distance of one chord far from the trailing edge, using pressure devices. The reduced frequency was set at 0.012, 0.03 and the motion pivot was selected at c/4.

Findings
Analysis of the steadiness of the wake flow field ascertains that an increase in reduced frequency leads to further flow time lag in the hold phase whereas decreases the time that the wake remains steady after the start of the return portion. Also, the roles of amplitude and stall condition are examined.

Practical implications
Examination of a pitch–hold–return motion is substantial in assessment of aerodynamics of maneuvers with a rapid increase in angle of attack. Moreover, study of aerodynamic behavior of downstream flow field and its steadiness in the wake of the airfoil is vital in drag reduction and control of flapping wings, dynamic stability and control of aircrafts.

Originality/value
In the present study, to discuss the steadiness of the flow field behind the airfoil some statistical methods and concept of histogram using an automatic algorithm were used and a specific criterion to characterize the steadiness of flow field was achieved.
Evolution of financial inclusion in Latin America
Authors: Martinez Lisana B. , Scherger Valeria , Guercio M. Belén , Orazi Sofía 
Published: June 2020

PurposeThis paper analyses the evolution of the financial inclusion and its main determinants in seven Latin American countries.Design/methodology/approachThe database used is the Global Findex from the World Bank for the latest data released that includes the years 2011 and 2014. The variables used are formal financial accounts, formal savings and formal credit as proxies of financial inclusion for the years of study. Moreover, the use of debit and credit cards is considered. The methodologies applied are the mean difference tests, in order to contrast the hypotheses of the inclusion evolution and binary probit regressions models.FindingsThe results of the analysis show that there is a positive evolution in the use of financial instruments in the countries of the sample, especially in the use of formal accounts. On the other hand, considering the characteristics of the individuals, age, level of education and income positively affect their financial inclusion.Originality/valueThere are no similar works for the region of study that allow us to evaluate the evolution of financial inclusion considering the variables selected in the literature. It is possible to clearly fulfil the proposed objective, highlighting the importance of implementing financial inclusion policies in view of the low percentage of use of the instruments in the analyzed countries.
As you sow, so shall you reap: finding customer-based outcomes of socially responsible coffee cafés
Authors: Ahmed Ishfaq , Islam Talat , Abdul Rasid Siti Zaleha , Anwar Farooq , Khalid Arooj 
Published: June 2020

PurposeIn the realm of increased competition among coffee café, the need for customer citizenship behavior (CCB) has increased. But the question of how a café can win CCB through firm-level and individual dispositional factors have not been gained due attention. Against this backdrop, this study aims to investigate a moderated-mediation model to predict CCB through CSR, affective commitment and service quality.Design/methodology/approachThe data were collected from 797 coffee café visitors between April–June 2019. The respondents were approached through a convenience sampling technique.FindingsUsing both Structural Equation Modeling and Hayes Macros, data analysis proved that CSR significantly predicts affective commitment and CCB. Moreover, it is also witnessed that the presence of high service quality strengthens the mediation mechanism; thus, a moderated-mediation model is supported.Research limitations/implicationsThe study is cross-sectional, although the design did not cause a common method variance. The model tested in the study offers a novel mechanism explaining the linking between CSR and CCB through affective commitment and service quality. The findings are useful for the management of café as the large sample size may offer generalizable results.Originality/valueThe study offers a novel way of predicting CCB, the desired customer behavior in the present competitive environment in the coffee market. The usefulness of the findings for management is yet another contribution offered by this study.
Receptacle interacts with consumers’ need for touch to influence tea-drinking experience
Authors: Wang Chujun , Peng Yubin , Spence Charles , Wan Xiaoang 
Published: June 2020

PurposeThis study was designed to investigate how the material properties of the tea-drinking receptacle interact with a participant's motivation and preference for extracting and using information obtained via haptic perception, namely the need for touch (NFT), to influence his or her tea-drinking experience.Design/methodology/approach72 blindfolded participants were instructed to sample room temperature tea beverages served in a cup that was made of ceramic, glass, paper or plastic. They were then asked to rate how familiar they were with the taste of the beverage, to rate how pleasant the taste was and to specify how much they would like to pay for it (i.e. willingness-to-pay ratings).FindingsThe material of the receptacles used to serve the tea exerted a significant influence over the pleasantness ratings of the tea and interacted with the participants' NFT, exerting a significant influence over their willingness to pay for the tea. Specifically, high-NFT participants were willing to pay significantly more for the same cup of tea when it was served in a ceramic cup rather than in a paper cup, whereas the low-NFT participants' willingness to pay for the tea was unaffected by the material of the receptacles.Originality/valueOur findings suggest that consumers may not be equally susceptible to the influence of the receptacle in which tea, or any other beverage, is served. Our findings also demonstrate how the physical properties of a receptacle interact with a consumer's motivation and preference to influence his or her behavior in the marketplace.
A product-technology portfolio alignment approach for food industry: a multi-criteria decision making with z-numbers
Authors: Garousi Mokhtarzadeh Nima , Amoozad Mahdiraji Hannan , Jafari-Sadeghi Vahid , Soltani Arash , Abbasi Kamardi AliAsghar 
Published: June 2020

PurposeTo design a novel hybrid approach to illustrate a reciprocal alignment to integrate future products and technologies. This mixed qualitative-quantitative method aims to optimize the final product portfolio and production technologies alignment in the food industry.Design/methodology/approachA list of products and technologies is extracted and evaluated by experts employing Market Attractiveness and Ease of Implementations Matrix (MA-EI) for products and attractiveness and technological Capability Matrix (A-C) for technologies. Weights of high-scored alternatives are attained applying the Z-number extension of Best Worst Method (ZBWM). After the product-technology matrix is formed and the alignment scores of each pair are determined by experts. Subsequently, final scores are computed, and a framework is proposed by electing high-ranked products and technology of each cluster to form the aligned product and technology portfolios of a food and hygiene industry company.FindingsBy employing an uncertain multicriteria decision-making approach besides product and technology matrices in a food industry corporation, among 40 technology and product, 13 products by 6 technologies are proposed. Thus, only six technology are necessary to manufacture the highly important and effective products.Originality/valueThe combination of product and technology analysis matrixes with an uncertain decision-making approach is considered as a novel approach in this research. Moreover, the distinctness between the present study and other researches is the concurrent unified aspect of product portfolio and technology optimization and its implementation in the planning discussion, especially in the food industry.
High pressure processing of apple juice: the most effective parameters to inactivate pathogens of reference
Authors: Rodrigues Petrus Rodrigo , Joseph Churey John , William Worobo Randy 
Published: June 2020

PurposeHigh-acid liquid foods are a substrate in which foodborne pathogens can maintain their viability. In this research an experimental design was conducted to optimize the parameters for high pressure processing (HPP) of apple juice (pH 3.76).Design/methodology/approachJuice was inoculated with cocktails of Escherichia coli O157:H7, Salmonella enterica and Listeria monocytogenes. Pressures ranging from 139 to 561 MPa and dwell times between 39 and 181 s were challenged.FindingsPressures above 400 MPa achieved a greater than 5-log reduction in all pathogen cocktails regardless of the dwell time. L. monocytogenes was more sensitive to HPP at a pressure of 350 MPa and dwell times equal to or beyond 110 s. E. coli O157:H7 and S. enterica exhibited similar resistance; the number of log reductions in the central point (350 MPa/110 s) ranged from 2.2 to 3.7. The first-order mathematical model better fitted experimental data for E. coli O157:H7 and S. enterica. In regard to L. monocytogenes, the second-order model better fitted this pathogen's reduction.Practical implicationsFruit juices are usually high pressure processed at approximately 600 MPa. For pathogenic reduction, the use of milder parameters may save energy and maintenance costs. The results herein exhibited could assist the apple juice industry with more effective applications of HPP.Originality/valueThe findings of this study demonstrate that relatively moderate pressures can be successfully used to assure the safety of apple juice.
A simulation model for managing customer waiting time in restaurants: scenarios and beyond
Authors: Tasar Bahar , Ventura Keti , Cicekli Ural Gokay 
Published: June 2020

PurposeThe purpose of this paper is to investigate the effects of capacity decisions regarding the number of servers/chefs and tables on identifying a change in the number of wait-related anxious customers, customer losses and customers served to meet the waiting time standards of an actual upscale restaurant.Design/methodology/approachThe authors applied a simulation model to present the consequences of restaurant capacity decisions based on waiting time standards. Arena Simulation Software, licensed by Rockwell Automation, was used for modeling and identifying distributions of the data set provided by the restaurant. An experiment was designed for an upscale restaurant with existing five servers/chefs and 50 tables by changing these resources to measure the changes in customers' wait-related anxiety and other service performance indicators.FindingsThe results showed that an additional server/chef on weekends decreases the daily average number of anxious customers by nearly 33% and increases the daily average number of customers served by nearly 3% and has a little positive effect of decreasing customer losses. Table insertion for high- and low-requested seating areas had an only positive effect on decreasing customer losses.Originality/valueIn this study, the service capacity is dependent on waiting time, and it is addressed to study the relationship with customers' wait-related anxiety, which is a subjective metric. This study developed a point of view for identifying anxious customers whose waiting times are much longer than their cooking and delivery duration expectations regarding their meal preferences in the cooking stage and waiting experiences in the service entry.
An assessment of e-book collection development practices among Romance language librarians
Authors: Reyes Betsaida M. , Devlin Frances A. 
Published: June 2020

Purpose
The purpose of this paper is to describe the collection development practices regarding e-books among librarians who manage French, Italian, Portuguese and Spanish (Romance) materials. The authors aim to describe factors that influence acquisition of e-books for Romance language collections to confirm librarians’ perception that humanities researchers prefer print and library administrators’ attitudes toward e-books.

Design/methodology/approach
This study collected data using a mixed-method approach of a survey and focus groups.

Findings
This study confirms that user preference is the primary consideration of Romance librarians in selecting e-books. Contrary to librarians’ perceptions, this study found that humanities faculty and students are not averse to using e-books for specific purposes such as searching, targeted reading and course materials. While restrictions on lending e-books are a concern, Romance librarians are focused primarily on serving the needs of their core constituencies.

Research limitations/implications
The practice of adding call numbers to individual e-books varies among institutions. Individual e-book titles in large packages do not necessarily get added to the catalog, thus making it very difficult to compare e-book collections between institutions.

Originality/value
This study endeavors to unify the anecdotal narratives and factors that influence the acquisition of e-books by Romance librarians.