bluuebunny/crossref_metadata_2025_split huggingface dataset:
Blockade of BDNF signalling attenuates chronic visceral hypersensitivity in an IBS‐like rat model
Authors: Fan Fei , Tang Ying , Dai Hengfen , Cao Yang , Sun Pei , Chen Yu , Chen Aiqin , Lin Chun 
Published: January 2020

AbstractBackgroundIrritable bowel syndrome (IBS) is a common functional disease characterized by chronic abdominal pain and changes in bowel movements. Effective therapy for visceral hypersensitivity in IBS patients remains challenging. This study investigated the roles of brain‐derived neurotrophic factor (BDNF) and tyrosine kinase receptor B (TrkB) and the effect of ANA‐12 (a selective antagonist of TrkB) on chronic visceral hypersensitivity in an IBS‐like rat model.MethodsAn IBS‐like rat model was established through neonatal maternal separation (NMS), and visceral hypersensitivity was assessed by electromyographic (EMG) responses of the abdominal external oblique muscles to colorectal distention (CRD). Different doses of ANA‐12 were injected intrathecally to investigate the effect of that drug on visceral hypersensitivity, and the open field test was performed to determine whether ANA‐12 had side effects on movement. Thoracolumbar spinal BDNF, TrkB receptor and Protein kinase Mζ (PKMζ) expression were measured to investigate their roles in chronic visceral hypersensitivity. Whole‐cell recordings were made from thoracolumbar superficial dorsal horn (SDH) neurons of lamina II.ResultsThe expression of BDNF and TrkB was enhanced in the thoracolumbar spinal cord of the NMS animals. ANA‐12 attenuated visceral hypersensitivity without side effects on motricity in NMS rats. PKMζ expression significantly decreased after the administration of ANA‐12. The frequency of spontaneous excitatory postsynaptic currents (sEPSCs) increased in the thoracolumbar SDH neurons of lamina II in NMS rats. The amplitude and frequency of sEPSCs were reduced after perfusion with ANA‐12 in NMS rats.ConclusionsNeonatal maternal separation caused visceral hypersensitivity and increased synaptic activity by activating BDNF‐TrkB‐PKMζ signalling in the thoracolumbar spinal cord of adult rats. PKMζ was able to potentiate AMPA receptor (AMPAR)‐mediated sEPSCs in NMS rats. ANA‐12 attenuated visceral hypersensitivity and synaptic activity by blocking BDNF/TrkB signalling in NMS rats.SignificanceANA‐12 attenuates visceral hypersensitivity via BDNF‐TrkB‐PKMζ signalling and reduces synaptic activity through AMPARs in NMS rats. This knowledge suggests that ANA‐12 could represent an interesting novel therapeutic medicine for chronic visceral hypersensitivity.
If you want to be one of us, then become like us: The evaluation of naturalization applicants by host nationals
Authors: Politi Emanuele , Roblain Antoine , Gale Jessica , Licata Laurent , Staerklé Christian 
Published: January 2020

AbstractImmigrant naturalization is a rite of passage, making assimilationist attitudes particularly pronounced among host nationals. Three experimental studies investigate whether heritage culture maintenance violates expectations that citizenship should be deserved by proving strong attachment to the host nation (i.e., neoliberal communitarianism). Study 1 (N = 293) demonstrates that naturalization applicants’ high degree of heritage culture maintenance impairs application evaluations. Perceived attachment to the host nation and citizenship deservingness mediated this effect. Study 2 (N = 220) replicates results across two national contexts and reveals that heritage culture maintenance impairs evaluations only among naturalization applicants from devalued countries. Study 3 (N = 117) manipulates attachment to the host nation and shows that perceived citizenship deservingness mediates the negative effects of naturalization applicants' low attachment to the host nation on application evaluations. Overall, assimilationist attitudes among host nationals are best explained by the combination of neoliberal and communitarian criteria of evaluation.
First application of CE‐ICP‐MS for monitoring the formation of cisplatin targeting delivery systems with gold nanocarriers
Authors: Wróblewska Anna , Matczuk Magdalena 
Published: January 2020

AbstractCisplatin is a drug frequently used in chemotherapy of various types of tumors due to its strong cytostatic activity against cancer cells. However, this therapy is not free from severe side effects related to the nonselective action of the drug. The solution to this problem could be the application of drug‐targeted delivery systems (DTDSs). Gold nanoparticles can be used in such systems as selective drug carriers, ensuring its transportation through the bloodstream to destination tissue. The method of DTDSs analysis providing qualitative and quantitative information about the formation of this conjugation is crucial to establish the kinetics of reaction and stoichiometry of reagents, which ensures the best drug binding rate. Moreover, the status of so far proposed techniques/methods dedicated to elaborating the course of DTDSs formation is preliminary and in majority guarantee only the confirmation of drug‒carrier conjugate formation. In this paper, we demonstrate the procedures of reagents’ preparation and cisplatin‒gold nanoparticles DTDS formation, which have a significant influence on the rate and stoichiometry of the reaction. We also present the novel application of CE‐ICP‐MS hyphenation for effective separation and online monitoring of all components of the reaction mixture.
Facial functional outcomes analysis after reconstruction by vascularized thoracodorsal nerve free flap following radical parotidectomy with facial nerve sacrifice
Authors: Bedarida Vincent , Qassemyar Quentin , Temam Stéphane , Janot François , Kolb Frédéric 
Published: January 2020

AbstractBackgroundParotid spread tumor may occasion wide defect with facial nerve sacrifice. We report our one time reconstruction experience of this defect using a thoracodorsal artery perforator and nerve flap (TAPN).MethodsEight patients underwent a radical parotidectomy with facial nerve sacrifice between February 2010 and June 2016. A single time reconstruction was performed using a thoracodorsal artery perforator and nerve flap, with skin or fat paddle. The thoracodorsal nerve vascularized was harvested and used to reconstruct the facial nerve from the trunk to four until six distal branches. Patients underwent physiotherapy for 3 months at least. Facial outcomes were assessed using House‐Brackmann scale and eFACE application.OutcomesMean follow‐up was 30 months. No complication occurred on donor site. All patients recovered a complete soft eye closure. No Frey syndrome occurred.ConclusionTAPN is adapted to wide and complex parotid defects.
Prognostic value of post‐ablation 131I scintigraphy in children with thyroid cancer
Authors: Zhang Xinyue , Liu Lina , Chen Yu , Huang Rui , Liu Bin 
Published: January 2020

AbstractBackgroundTo evaluate the prognostic value of post‐ablation whole‐body scintigraphy (PAS) in children with thyroid cancer following 131I therapy.MethodsA retrospective analysis of children with thyroid cancer who underwent remnant ablation was performed. PAS results were correlated with clinical and histopathologic characteristics of the primary tumor and compared with the follow‐up data.ResultsOne hundred and sixty‐eight children were eligible for the analysis. The median age was 17 years, and the majority were female (81.5%). In multivariate analysis, N1b classification and 131I ablative dose were associated with PAS positivity. After a median follow‐up of 49 months, 89 (71.8%) of 124 children with normal PAS achieved complete remission. Fifteen of 44 (34.1%) children with positive PAS had complete remission. Kaplan‐Meier analysis showed that positive PAS was negatively associated with cumulative complete remission rates (P < .001).ConclusionsPAS could provide prognostic information in children with thyroid cancer undergoing 131I therapy.
Pd‐Catalyzed C−C, C−N, and C−O Bond‐Forming Difunctionalization Reactions of Alkenes Bearing Tethered Aryl/Alkenyl Triflates
Authors: White Derick R. , Bornowski Evan C. , Wolfe John P. 
Published: January 2020

AbstractOver the past few years our group has described a new type of alkene difunctionalization reaction in which aryl or alkenyl triflates bearing tethered alkenes are coupled with various nucleophiles to afford carbocyclic products. The products are formed in moderate to good chemical yield, with generally high levels of stereoselectivity. Our progress to date in this area, which includes reactions of amine, alcohol, enolate, and indole nucleophiles, is described in this review.
Women's reluctance for pregnancy: Experiences and perceptions of Zika virus in Medellin, Colombia
Authors: Tirado Veronika , Morales Mesa Santiago A. , Kinsman John , Ekström Anna Mia , Restrepo Jaramillo Berta N. 
Published: January 2020

AbstractObjectiveTo explore how being infected with the Zika virus during pregnancy was experienced by affected women, and how it influenced their family relationships and future family planning.MethodsWe conducted a qualitative study, including 19 semistructured interviews with women of reproductive age and confirmed Zika infection during 2015–2018 in Medellin, Colombia. Purposeful sampling was applied, and participants were identified through National Public Health Surveillance System records. Interviews were recorded, transcribed verbatim, and analyzed using content analysis with inductive and deductive approaches.ResultsOf 19 women interviewed, eight women identified the pregnancy as unexpected and two women had undergone permanent sterilization. Women had mixed views about decision‐making related to family planning, and not having an abortion in a future pregnancy was influenced by religious beliefs. Women knew about vector‐borne transmission but were not well informed about sexual transmission of the virus. Women desired better support and guidance to ease concerns about Zika virus.ConclusionAll interviewed women expressed a need for more information about Zika virus and continuous support, specifically after delivery, from healthcare professionals. Communication strategies to enhance culturally sensitive messages and for accurate perception of information are recommended during Zika outbreaks.
Analysis of the spatial distribution of cases of Zika virus infection and congenital Zika virus syndrome in a state in the southeastern region of Brazil: Sociodemographic factors and implications for public health
Authors: Mocelin Helaine J.S. , Catão Rafael C. , Freitas Paula S.S. , Prado Thiago N. , Bertolde Adelmo I. , Castro Marcia C. , Maciel Ethel L.N. 
Published: January 2020

AbstractObjectiveTo perform spatial distribution analysis of reported cases of Zika virus and congenital Zika syndrome (CZS) in the state of Espírito Santo, Brazil, by neighborhood, and relate the results to sociodemographic indicators and implications for the health process.MethodsAn ecological study using data from the 2016 National Notifiable Diseases Surveillance System, epidemiological records, and information on neighborhoods of families confirmed with CZS from qualitative field research.ResultsSociodemographic indicators were analyzed in three distinct groups: general population with Zika virus, pregnant women with Zika virus, and cases of CZS visited. For the three groups, average literacy rates were 71.1%, 71.0%, and 68.3%; the average income per minimum wage was 1.4, 1.1, and 1.4; sanitary sewage coverage was 75.6%, 76.1%, and 71.4%; garbage coverage was 90.8%, 91.2%, and 89.2%; and water supply was 93.8%, 94.1% and 93.8%, respectively. Socioeconomic indicators showed no significant differences between groups, although they were above the national average. A nonsignificant variation of 68.3%–71.1% was seen in the average literacy level above 15 years of age.ConclusionSocioeconomic and demographic indicators of cases of Zika virus infection and CZS may indicate that the outbreak had different impacts according to class, social group, or gender, reflecting the persistence and social geography of inequality in Brazil.
Neurological complications associated with emerging viruses in Brazil
Authors: Angelo Jussara R. , Fuller Trevon L. , Leandro Bianca B.S. , Praça Heitor L.F. , Marques Renata D. , Ferreira João M.C. , Pupe Camila C.B. , Perez Olívia C. , Nielsen‐Saines Karin , Nascimento Osvaldo J.M. , Sabroza Paulo C. 
Published: January 2020

AbstractObjectiveTo test the hypotheses that emerging viruses are associated with neurological hospitalizations and that statistical models can be used to predict neurological sequelae from viral infections.MethodsAn ecological study was carried out to observe time trends in the number of hospitalizations with inflammatory polyneuropathy and Guillain‐Barré syndrome (GBS) in the state of Rio de Janeiro from 1997 to 2017. Increases in GBS from month to month were assessed using a Farrington test. In addition, a cross‐sectional study was conducted analyzing 50 adults hospitalized for inflammatory polyneuropathies from 2015 to 2017. The extent to which Zika virus symptoms explained GBS hospitalizations was evaluated using a calibration test.ResultsThere were significant increases (Farrington test, P<0.001) in the incidence of GBS following the introduction of influenza A/H1N1 in 2009, dengue virus type 4 in 2013, and Zika virus in 2015. Of 50 patients hospitalized, 14 (28.0%) were diagnosed with arboviruses, 9 (18.0%) with other viruses, and the remainder with other causes of such neuropathies. Statistical models based on cases of emerging viruses accurately predicted neurological sequelae, such as GBS.ConclusionThe introduction of novel viruses increases the incidence of inflammatory neuropathies.
In vitro effect of vanadyl sulfate on cultured primary astrocytes: cell viability and oxidative stress markers.
Authors: Ścibior Agnieszka , Szychowski Konrad A. , Zwolak Iwona , Dachowska Klaudia , Gmiński Jan 
Published: January 2020

AbstractExposure to vanadium has been associated with deleterious effects on the central nervous system in animals and humans. Although vanadium‐derived pro‐oxidant species were reported to be involved in vanadium‐mediated neurotoxicity, the ability of this metal to induce oxidative stress markers in glial cells remains to be elucidated. In this study, we investigated the cytotoxicity and the generation of reactive oxygen species (ROS) and nitric oxide (NO) by mouse primary astrocytes after treatment with vanadyl sulfate (VOSO4) at concentrations of 20, 50, 100, 200, and 500 μM. The resazurin assay revealed that treatment with VOSO4 for 24 and 48 h at concentrations of 50 and 100 μM, respectively, or higher substantially induced astrocytic cytotoxicity. Intracellular ROS increased after 6‐h exposure to the lowest concentration tested (20 μM VOSO4) and tended to intensify after 24‐ and 48‐h treatments reaching significant values for 20 and 500 μM VOSO4. In turn, NO production in the examined cells was elevated after exposure to all concentrations at the 6‐, 24‐, and 48‐h incubation periods. Our study demonstrated the ability of VOSO4 to induce H2O2 generation in cell‐free DMEM/F12 medium. The H2O2 levels were in the micromolar range (up to 5 μM) and were detected mostly during the first few minutes after VOSO4 addition, suggesting that the generated H2O2 could not induce toxic effects on the cells. Taken together, these results show VOSO4 induced cytotoxicity in primary astrocyte cells, which may have resulted from vanadyl‐stimulated intracellular ROS and NO generation in these cells.
Ameliorating effects of agomelatine on testicular and epididymal damage induced by methotrexate in rats
Authors: Aslankoc Rahime , Ozmen Ozlem , Ellidag Hamit Yasar 
Published: January 2020

AbstractThe aim of this study was to investigate the possible prophylactic effects of agomelatine (AGO) against testicular and epididymal damage induced by methotrexate (MTX) in rats. Twenty‐four male Wistar albino rats were divided into three groups: Group I (control group), Group II (MTX group: 20 mg/kg MTX, i.p, single dose), and Group III (MTX+AGO group: 20 mg/kg MTX, i.p, single dose+40 mg/kg AGO; gavage, 7 days). The rats were killed under anesthesia 24 hours after the last AGO application. Testicular and epididymal tissues were bilaterally removed for morphometric, biochemical, pathological, and immunohistochemical analyses. Body, testicular, and epididymal weights were measured. Malondialdehyde (MDA), superoxide dismutase, catalase, and glutathione peroxidase levels were measured in testes. Sperm count, hyperemia, edema, inflammatory reaction, degenerated and necrotic cells were evaluated by histopathological analysis. In addition, inducible nitric oxide synthase (iNOS), granulocyte colony‐stimulating factor (G‐CSF), osteopontin (OPN), and heat shock protein‐70 (HSP70) immune reactions were analyzed in testes and epididymides. Decreased epididymal weights, increased MDA levels, decreased sperm count, hyperemia, edema, inflammatory reaction, and degenerated and necrotic cells were observed in the MTX group. In addition, iNOS, HSP70, G‐CSF, and OPN immune reactions were increased. AGO improved morphometric, biochemical, histopathological, and immunohistochemical findings. The present study confirms that MTX induces testicular and epididymal damage both biochemically and immunohistochemically. However, AGO demonstrated ameliorative effects on both biochemical and pathological findings of the current study.
Lansoprazole enhances the antidiabetic effect of dapagliflozin in fortified diet‐fed streptozotocin‐treated diabetic rats
Authors: Gamil Noha M. , Abd El Fattah Mai A. , Ahmed Maha A. E. , Maklad Yousreya A. , Gamal el Din Amina A. , Eid Nihad I. 
Published: January 2020

AbstractDapagliflozin (DAPA) is used for treating type 2 diabetes, whereas lansoprazole (LPZ) is used as a traditional antiulcer drug. The present study investigated the possible antidiabetic effects of LPZ on fortified diet‐fed streptozotocin (FDF/STZ)‐induced insulin‐resistant diabetic rats. On the basis of the current results, it can be concluded that LPZ could be used as an add‐on drug along with the conventional treatment for T2D as it showed beneficial effects in the current experimental model of insulin resistance.
Three‐site and five‐site fixed‐charge water models compatible with AMOEBA force field
Authors: Pan Cong , Liu Chengwen , Peng Junhui , Ren Pengyu , Huang Xuhui 
Published: January 2020

AbstractIn a typical biomolecular simulation using Atomic Multipole Optimized Energetics for Biomolecular Applications (AMOEBA) force field, the vast majority molecules in the simulation box consist of water, and these water molecules consume the most CPU power due to the explicit mutual induction effect. To improve the computational efficiency, we here develop two new nonpolarizable water models (with flexible bonds and fixed charges) that are compatible with AMOEBA solute: the 3‐site AW3C and 5‐site AW5C. To derive the force‐field parameters for AW3C and AW5C, we fit to six experimental liquid thermodynamic properties: liquid density, enthalpy of vaporization, dielectric constant, isobaric heat capacity, isothermal compressibility and thermal expansion coefficient, at a broad range of temperatures from 261.15 to 353.15 K under 1.0 atm pressure. We further validate our AW3C and AW5C water models by showing that they can well reproduce the radial distribution function g(r), self‐diffusion constant D, and hydration free energy from the AMOEBA03 water model and the experimental observations. Furthermore, we show that our AW3C and AW5C water models can greatly accelerate (>5 times) the bulk water as well as biomolecular simulations when compared to AMOEBA water. Specifically, we demonstrate that the applications of AW3C and AW5C water models to simulate a DNA duplex lead to a threefold acceleration, and in the meanwhile well maintain the structural properties as the fully polarizable AMOEBA water. We expect that our AW3C and AW5C water models hold great promise to be widely applied to simulate complex bio‐molecules using the AMOEBA force field.
Portuguese version of the Posttraumatic Stress Disorder Checklist for DSM‐5 (PCL‐5): Comparison of latent models and other psychometric analyses
Authors: Carvalho Teresa , da Motta Carolina , Pinto‐Gouveia José 
Published: January 2020

AbstractObjectiveThis psychometric study explores the Portuguese version of the Posttraumatic Stress Disorder (PTSD) Checklist (PCL‐5). It aims to clarify the best‐fitting latent structure among competing PTSD models (Diagnostic and Statistical Manual of Mental Disorders‐fifth edition [DSM‐5], Dysphoria, Dysphoric Arousal, Anhedonia, Externalizing Behavior, And Hybrid models) and its implications for PTSD measurement.MethodPsychometric analyses were conducted in a sample from the general population of firefighters (N = 446), except the temporal stability, which was tested in a subsample of 100 participants.ResultsThe models presented significant differences in a global fit. The Hybrid model presented the best‐fitting structure, but the DSM‐5 model showed more favorable reliability and convergent validity in Confirmatory Factor Analyses. The DSM‐5 model also proved to be internally consistency, temporally stable, and presented convergent validity.ConclusionThe Portuguese version of PCL‐5 is reliable and valid. The findings suggest the appropriateness of the DSM‐5 model to assess PTSD symptomatology, encouraging its use in clinical, and research settings.
Eukaryotic initiation Factor 4AIII facilitates hepatocellular carcinoma cell proliferation, migration, and epithelial‐mesenchymal transition process via antagonistically binding to WD repeat domain 66 with miRNA‐2113
Authors: Zhang Li , Chen Yangzong , Bao Chunchun , Zhang Xiuxing , Li Haiying 
Published: January 2020

AbstractHepatocellular carcinoma (HCC) is one of the malignant cancers with high incidence and mortality rates worldwide. RNA‐binding protein eukaryotic initiation Factor 4A‐III (eIF4AIII) is a carcinogene in the biological process of tumors and microRNA (miRNA)‐2113 has rarely been studied in cancers, not to mention in HCC. The regulation mechanism between eIF4AIII and miR‐2113 involved in HCC is yet to be explored. The purpose of this research is to probe the function role and associated underlying mechanism of eIF4AIII participated in HCC. The results revealed that eIF4AIII was overexpressed in HCC. Lost‐of‐function assays found that eIF4AIII knockdown, WD (Trp‐Asp [tryptophan and asparaginic acid]) repeat domain 66 (WDR66) silence or miR‐2113 promotion repressed cell proliferation, migration, and epithelial‐mesenchymal transition (EMT) process in HCC. Furthermore, eIF4AIII could interact with WDR66 and further stabilize WDR66 messenger RNA. In addition, WDR66 was a target gene of miR‐2113. Besides, WDR66 was antagonistically regulated by eIF4AIII and miR‐2113. Rescue assays verified that eIF4AIII promoted HCC cell proliferation, migration, and EMT process via antagonistically binding to WDR66 with miR‐2113. Taken together, these findings indicated an important role and a novel mechanism of eIF4AIII in HCC, providing an optional therapy for HCC patients.