bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nutritive values of double-low rapeseed expellers and rapeseed meal with or without supplementation of multi-enzyme in pigs
Authors: Li Peili , Lyu Zhiqian , Wang Lu , Huang Bingbing , Lai Changhua 
Published: November 2020

Two experiments were conducted to evaluate the effects of adding multi-enzyme on the available energy concentration and standardized ileal digestibility (SID) of amino acids of double-low rapeseed expellers (RSE) and rapeseed meal (RSM) fed to growing pigs. In Experiment 1, a total of 36 growing barrows were fed six diets in a replicated 3 × 2 factorial design. Diets included a corn–soybean meal basal diet, RSE diet, and RSM diet with or without multi-enzyme (1000 U g−1 of cellulase, 10 000 U g−1 of xylanase, 2000 U g−1 of glucanase, and 10 000 U g−1 of protease). In Experiment 2, a total of 30 crossbred pigs were randomly allotted to five diets consisting of a nitrogen-free diet and four cornstarch-based diets containing RSE or RSM with or without multi-enzyme. Multi-enzyme supplementation significantly improved (P < 0.05) the metabolizable energy (ME) value and apparent total tract digestibility (ATTD) of ether extract, neutral detergent fiber, and acid detergent fiber, and it tended to increase (P < 0.10) the ATTD of gross energy and digestible energy in ingredients. With the exception of phenylalanine, multi-enzyme supplementation increased (P < 0.05) the SID of crude protein (CP) and all amino acids (AA) in ingredients. Multi-enzyme supplementation enhanced fiber degradation and increased the ME value and SID of CP as well as most AA in double-low rapeseed co-products fed to pigs.
Atlantic bluefin tuna (Thunnus thynnus) in Greenland — mixed-stock origin, diet, hydrographic conditions, and repeated catches in this new fringe area
Authors: Jansen Teunis , Nielsen Einar Eg , Rodriguez-Ezpeleta Naiara , Arrizabalaga Haritz , Post Søren , MacKenzie Brian R. 
Published: November 2020

Based on collaboration with the Greenlandic fishing fleet, we document the presence of Atlantic bluefin tuna (Thunnus thynnus) in most years from 2012 to 2018 in the waters east of Greenland (northern Irminger Sea). In total, 84 individuals have been registered as bycatch in the commercial fisheries in Greenland waters, which indicates that the first catch of three individuals in 2012 was not a single extreme observation, but that East Greenland waters have become a new outer limit of an expanded tuna habitat. Genetic analyses indicate that specimens from this region are mostly of Mediterranean origin with a small proportion originating from the Gulf of Mexico stock. Stomach content analysis suggests that the main prey is Atlantic mackerel (Scomber scombrus). The tunas ranged in size from 140 to 270 cm, corresponding to an estimated age range of 5–16 years; most were probably mature. The wide size–age range suggests that many year classes are participating in the migration to this region. Sea temperatures during summer have been above the long-term average in recent years of interest. Summer residence of bluefin tuna in the region could be due to a combination of increasing temperatures and higher overall abundances of both bluefin tuna and a key prey species (Atlantic mackerel).
Eph Receptors in the Immunosuppressive Tumor Microenvironment
Authors: Janes Peter W. , Vail Mary E. , Ernst Matthias , Scott Andrew M. 
Published: November 2020

Abstract
The tumor microenvironment (TME) promotes tumor development via complex intercellular signaling, aiding tumor growth and suppressing immunity. Eph receptors (Eph) and their ephrin ligands control cell interactions during normal development, and reemerge in tumors and the TME, where they are implicated in invasion, metastasis, and angiogenesis. Recent studies also indicate roles for Ephs in suppressing immune responses by controlling tumor interactions with innate and adaptive immune cells within the TME. Accordingly, inhibiting these functions can promote immune response and efficacy of immune checkpoint inhibition. This research highlights Ephs as potential targets to enhance efficacy of immune-based therapies in patients with cancer.
Rapid Induction of the Unfolded Protein Response and Apoptosis by Estrogen Mimic TTC-352 for the Treatment of Endocrine-Resistant Breast Cancer
Authors: Abderrahman Balkees , Maximov Philipp Y. , Curpan Ramona F. , Fanning Sean W. , Hanspal Jay S. , Fan Ping , Foulds Charles E. , Chen Yue , Malovannaya Anna , Jain Antrix , Xiong Rui , Greene Geoffrey L. , Tonetti Debra A. , Thatcher Gregory R.J. , Jordan V. Craig 
Published: November 2020

AbstractPatients with long-term estrogen-deprived breast cancer, after resistance to tamoxifen or aromatase inhibitors develops, can experience tumor regression when treated with estrogens. Estrogen's antitumor effect is attributed to apoptosis via the estrogen receptor (ER). Estrogen treatment can have unpleasant gynecologic and nongynecologic adverse events; thus, the development of safer estrogenic agents remains a clinical priority. Here, we study synthetic selective estrogen mimics (SEM) BMI-135 and TTC-352, and the naturally occurring estrogen estetrol (E4), which are proposed as safer estrogenic agents compared with 17β-estradiol (E2), for the treatment of endocrine-resistant breast cancer. TTC-352 and E4 are being evaluated in breast cancer clinical trials. Cell viability assays, real-time PCR, immunoblotting, ERE DNA pulldowns, mass spectrometry, X-ray crystallography, docking and molecular dynamic simulations, live cell imaging, and Annexin V staining were conducted in 11 biologically different breast cancer models. Results were compared with the potent full agonist E2, less potent full agonist E4, the benchmark partial agonist triphenylethylene bisphenol (BPTPE), and antagonists 4-hydroxytamoxifen and endoxifen. We report ERα's regulation and coregulators’ binding profiles with SEMs and E4. We describe TTC-352′s pharmacology as a weak full agonist and antitumor molecular mechanisms. This study highlights TTC-352′s benzothiophene scaffold that yields an H-bond with Glu353, which allows Asp351-to-helix 12 (H12) interaction, sealing ERα's ligand-binding domain, recruiting E2-enriched coactivators, and triggering rapid ERα-induced unfolded protein response (UPR) and apoptosis, as the basis of its anticancer properties. BPTPE's phenolic OH yields an H-Bond with Thr347, which disrupts Asp351-to-H12 interaction, delaying UPR and apoptosis and increasing clonal evolution risk.
Implementation of Risk-reducing Strategies for Breast Cancer is Long Overdue
Authors: Vogel Victor G. 
Published: November 2020

Abstract
Despite strong evidence that it is efficacious, chemoprevention has been underused in eligible women. Reasons offered not to adopt and initiate strategies to reduce the risk of breast cancer include the fear of adverse effects, medication costs, lack of reasonably accurate and feasible methods for assessing an individual's personal risk, and lack of established risk thresholds that maximize benefit and minimize harms. The article by Macdonald and colleagues remind us that the problem of lack of uptake of risk-reducing medications for breast cancer remains a worldwide clinical challenge despite endorsements from national and international organizations that recommend the use of risk-reducing medications for breast cancer with level I evidence. Several strategies are suggested to improve uptake and utilization of safe and effective chemoprevention medications with high therapeutic indices.
See related article by Macdonald et al., p. 131
Effect of Empagliflozin on Cardiovascular and Renal Outcomes in Patients With Heart Failure by Baseline Diabetes Status
Authors: Anker Stefan D. , Butler Javed , Filippatos Gerasimos , Khan Muhammad Shahzeb , Marx Nikolaus , Lam Carolyn S.P. , Schnaidt Sven , Ofstad Anne Pernille , Brueckmann Martina , Jamal Waheed , Bocchi Edimar A. , Ponikowski Piotr , Perrone Sergio V. , Januzzi James L. , Verma Subodh , Böhm Michael , Ferreira João Pedro , Pocock Stuart J. , Zannad Faiez , Packer Milton ,   On behalf of the EMPEROR-Reduced Trial Committees and Investigators
Published: November 2020

Background:
Sodium-glucose cotransporter 2 inhibitors improve outcomes in patients with heart failure with reduced ejection fraction, but additional information is needed about whether glycemic status influences the magnitude of their benefits on heart failure and renal events.

Methods:
Patients with Class II–IV heart failure and a left ventricular ejection fraction ≤40% were randomized to receive empagliflozin (10 mg daily) or placebo in addition to recommended therapy. We prespecified a comparison of the effect of empagliflozin in patients with and without diabetes.

Results:

Of the 3730 patients enrolled, 1856 (50%) had diabetes, 1268 (34%) had prediabetes (hemoglobin A1c [HbA1c] 5.7–6.4%), and 606 (16%) had normoglycemia (HbA1c <5.7%). The risks of the primary outcome (cardiovascular death or hospitalization for heart failure), total hospitalizations for heart failure, and adverse renal outcomes were higher in patients with diabetes, but were similar between patients with prediabetes and normoglycemia. Empagliflozin reduced the risk of the primary outcome in patients with and without diabetes (hazard ratio, 0.72 [95% CI, 0.60–0.87] and 0.78 [95% CI, 0.64–0.97], respectively,
P
-interaction=0.57). Patients with and without diabetes also did not differ with respect to the effect of empagliflozin on total hospitalizations for heart failure, on the decline in estimated glomerular filtration rate over time, and on the risk of serious adverse renal outcomes. Among these end points, the effects of the drug did not differ in patients with prediabetes or normoglycemia. When analyzed as a continuous variable, baseline HbA1c did not significantly modify the benefits of empagliflozin on the primary outcome (
P
-interaction=0.40). Empagliflozin did not lower HbA1c in patients with prediabetes or normoglycemia and was not associated with increased risk of hypoglycemia.

Conclusions:
In EMPEROR-Reduced (Empagliflozin Outcome Trial in Patients With Chronic Heart Failure With Reduced Ejection Fraction), empagliflozin significantly improved cardiovascular and renal outcomes in patients with heart failure and a reduced ejection fraction, independent of baseline diabetes status and across the continuum of HbA1c.

Registration:

URL:
https://www.clinicaltrials.gov
; Unique identifier: NCT03057977.
Contrastive Pragmatics and Second Language (L2) Pragmatics: Approaches to Assessing L2 Speech Act Production
Authors: Taguchi Naoko , Li Shuai 
Published: November 2020

Abstract
This state-of-the-art paper discusses common approaches to the assessment of pragmatic competence. Two approaches have dominated the assessment practice in the field of second language (L2) pragmatics. One approach, rooted in the tradition of contrastive pragmatics, involves comparing and contrasting L2 learners’ pragmalinguistic forms with those of native speakers to determine whether L2 forms approximate native speaker forms. The other approach, rooted in the tradition of performance-based assessment, involves using a rating scale to evaluate L2 pragmatic performance based on multiple criteria (e.g., clarity of intention, formality level of expressions, and interaction abilities). Focusing on the area of speech act assessment, this paper presents an overview of these two approaches, highlighting their advantages and disadvantages. By doing so, the paper intends to illustrate the interface between contrastive pragmatics and L2 pragmatics. The paper concludes with critical insights in terms of what is missing in these approaches under the current trend of globalization and intercultural communication.
Expected values for gastrointestinal and pancreatic hormone concentrations in healthy volunteers in the fasting and postprandial state
Authors: Meek Claire L , Lewis Hannah B , Burling Keith , Reimann Frank , Gribble Fiona 
Published: November 2020

Background Gastrointestinal hormones regulate intestinal transit, control digestion, influence appetite and promote satiety. Altered production or action of gut hormones, including glucagon-like peptide-1 (GLP-1), glucose-dependent insulinotropic polypeptide (GIP) and peptide YY (PYY), may contribute to the biological basis of obesity and altered glucose homeostasis. However, challenges in analytical methodology and lack of clarity on expected values for healthy individuals have limited progress in this field. The aim of this study was to describe expected concentrations of gastrointestinal and pancreatic hormones in healthy volunteers following a standardized meal test (SMT) or 75 g oral glucose tolerance test (OGTT). Methods A total of 28 healthy volunteers (12 men, 16 women; mean age 31.3 years; mean body mass index 24.9 kg/m2) were recruited to attend a hospital clinic on two occasions. Volunteers had blood sampling in the fasting state and were given, in randomized order, an oral glucose tolerance test (OGTT) and standardized mixed liquid meal test with venepuncture at timed intervals for 4 h after ingestion. Analytical methods for gut and pancreatic hormones were assessed and optimized. Concentrations of gut and pancreatic hormones were measured and used to compile ranges of expected values. Results Ranges of expected values were created for glucose, insulin, glucagon, GLP-1, GIP, PYY and free fatty acids in response to a standardized mixed liquid meal or OGTT. Intact proinsulin and C-peptide levels were also measured following the OGTT. Conclusions These ranges of expected values can now be used to compare gut hormone concentrations between healthy individuals and patient groups.
Posterior Pharyngeal Wall Tuberculosis—A Forgotten Entity Mimicking Malignancy
Authors: Kairo Arvind Kumar , Kajal Smile , Shamim Shamim Ahmed , Barwad Adarsh 
Published: November 2020

Background: Tuberculosis (TB) of posterior pharyngeal wall (PPW), either primary or secondary, is extremely rare and can mimic malignancy in elderly patients. Case Report: A 36-year-old female patient presented with complains of nonhealing ulcer at the back of her throat for 4 months. On examination, there was an ulceroproliferative growth covered with slough over PPW. Positron emission tomography scan showed uptake in PPW. There was no uptake in any other part of body. A biopsy from PPW lesion showed epithelioid cell granulomas with Langhans type giant cells. Auramine-rhodamine staining showed few beaded bacilli consistent with TB. Conclusion: Tuberculosis of PPW is an extremely uncommon entity and can mimic malignancy. It should be kept as one of the differential diagnosis as it can be cured solely by giving anti-tubercular therapy.
ECMO implantation training: Needle penetration in 3D printable materials and porcine aorta
Authors: Salewski Christoph , Spintzyk Sebastian , von Steuben Thore , Sandoval Boburg Rodrigo , Nemeth Attila , Schille Christine , Acharya Metesh , Geis-Gerstorfer Jürgen , Wendel Hans-Peter , Popov Aron-Frederik , Schlensak Christian 
Published: November 2020

Aim Patients with cardiogenic shock or ARDS, for example, in COVID-19/SARS-CoV-2, may require extracorporeal membrane oxygenation (ECMO). An ECLS/ECMO model simulating challenging vascular anatomy is desirable for cannula insertion training purposes. We assessed the ability of various 3D-printable materials to mimic the penetration properties of human tissue by using porcine aortae. Methods: A test bench for needle penetration and piercing in sampled porcine aorta and preselected 3D-printable polymers was assembled. The 3D-printable materials had Shore A hardness of 10, 20, and 50. 17G Vygon 1.0 × 1.4 mm × 70 mm needles were used for penetration tests. Results: For the porcine tissue and Shore A 10, Shore A 20, and Shore A 50 polymers, penetration forces of 0.9036 N, 0.9725 N, 1.0386 N, and 1.254 N were needed, respectively. For piercing through the porcine tissue and Shore A 10, Shore A 20, and Shore A 50 polymers, forces of 0.8399 N, 1.244 N, 1.475 N, and 1.482 N were needed, respectively. ANOVA showed different variances among the groups, and pairwise two-tailed t-tests showed significantly different needle penetration and piercing forces, except for penetration of Shore A 10 and 20 polymers (p = 0.234 and p = 0.0857). Significantly higher forces were required for all other materials. Conclusion: Shore A 10 and 20 polymers have similar needle penetration properties compared to the porcine tissue. Significantly more force is needed to pierce through the material fully. The most similar tested material to porcine aorta for needle penetration and piercing in ECMO-implantation is the silicon Shore A 10 polymer. This silicon could be a 3D-printable material in surgical training for ECMO-implantation.
Survivorship and Patient-Reported Outcomes After Comprehensive Arthroscopic Management of Glenohumeral Osteoarthritis: Minimum 10-Year Follow-up
Authors: Arner Justin W. , Elrick Bryant P. , Nolte Philip-C. , Haber Daniel B. , Horan Marilee P. , Millett Peter J. 
Published: November 2020

Background: Few long-term outcome studies exist evaluating glenohumeral osteoarthritis (GHOA) treatment with arthroscopic management. Purpose: To determine outcomes, risk factors for failure, and survivorship for the comprehensive arthroscopic management (CAM) procedure for the treatment of GHOA at minimum 10-year follow-up. Study Design: Case series; Level of evidence, 3. Methods: The CAM procedure was performed on a consecutive series of patients with advanced GHOA who opted for joint preservation surgery and otherwise met criteria for total shoulder arthroplasty. At minimum 10-year follow-up, postoperative outcome measures included change in the American Shoulder and Elbow Surgeons, Single Assessment Numeric Evaluation, 12-Item Short Form Health Survey (SF-12) Physical Component Summary, and visual analog scale for pain, along with the QuickDASH (shortened version of Disabilities of the Arm, Shoulder and Hand) and satisfaction score. Kaplan-Meier survivorship analysis was performed, with failure defined as progression to arthroplasty. Results: In total, 38 CAM procedures were performed with 10-year minimum follow-up (range, 10-14 years) with a mean patient age of 53 years (range, 27-68 years) at the time of surgery. Survivorship was 75.3% at 5 years and 63.2% at minimum 10 years. Those who progressed to arthroplasty did so at a mean 4.7 years (range, 0.8-9.6 years). For those who did not undergo arthroplasty, American Shoulder and Elbow Surgeons scores significantly improved postoperatively at 5 years (63.3 to 89.6; P < .001) and 10 years (63.3 to 80.6; P = .007). CAM failure was associated with severe preoperative humeral head incongruity in 93.8% of failures as compared with 50.0% of patients who did not go on to arthroplasty ( P = .008). Median satisfaction was 7.5 out of 10. Conclusion: Significant improvements in patient-reported outcomes were sustained at minimum 10-year follow-up in young patients with GHOA who underwent a CAM procedure. The survivorship rate at minimum 10-year follow-up was 63.2%. Humeral head flattening and severe joint incongruity were risk factors for CAM failure. The CAM procedure is an effective joint-preserving treatment for GHOA in appropriately selected patients, with sustained positive outcomes at 10 years.
Radiographic Reference Points Do Not Ensure Anatomic Femoral Fixation Sites in Medial Patellofemoral Ligament Reconstruction: A Quantified Anatomic Localization Method Based on the Saddle Sulcus
Authors: Chen Jiebo , Han Kang , Jiang Jia , Huangfu Xiaoqiao , Zhao Song , Zhao Jinzhong , Xie Guoming 
Published: November 2020

Background: Medial patellofemoral ligament (MPFL) reconstruction is one of the main treatments for lateral patellar translation. Based on intraoperative true lateral radiographs, the accepted methods for femoral MPFL tunnel location are potentially inaccurate. Direct assessment of anatomic characteristics during surgery through palpation of the anatomic landmarks involving the saddle sulcus might help eliminate tunnel malposition. Hypothesis: The saddle sulcus is a reliable osseous landmark where the MPFL attaches for tunnel placement. Study Design: Descriptive laboratory study. Methods: A total of 9 fresh-frozen unpaired human cadaveric knees were dissected; MPFL insertion point and relative osseous structures were marked. Three-dimensional images and transformed true lateral radiographs were obtained for analysis; 3 previously reported radiographic reference points for MPFL femoral tunnel placement were determined on all images and compared with the anatomic insertion. Results: A saddle sulcus consistently existed where the MPFL was attached, located at 11.7 ± 5.9 mm from the apex of the adductor tubercle (AT) to the medial epicondyle (ME), 62.8% of the average distance between the apexes of the AT and ME, and 5.6 ± 2.8 mm perpendicular-posterior to the border connecting the AT and ME. The reported radiographic reference points were located at average distances of 6.2 ± 3.2 mm (Schöttle method), 5.9 ± 2.3 mm (Redfern method), and 7.3 ± 6.6 mm (Fujino method) from the saddle sulcus center on the true lateral radiographs. Conclusion: The saddle sulcus was a reliable landmark where the MPFL was anatomically attached, located approximately 12 mm from the AT to the ME (approximately 60% along a line from the AT to the ME) and 6 mm perpendicular-posterior to the border connecting the apexes of the AT and ME. Additionally, the saddle sulcus position presented variability on the femoral aspect of different knees. All of the average direct distances from the sulcus to the reference radiographic points exceeded 5 mm, and tunnel localizations on a true lateral radiograph were inaccurate. Clinical Relevance: This study demonstrates the potential precise position of the saddle sulcus, according to the ME and AT, as a reliable anatomic landmark for MPFL femoral tunnel location. Radiographic reference points were not accurate during MPFL reconstruction. Direct palpation of the landmarks might be effective for femoral MPFL tunnel placement.
Harrodian Instability: An Unhelpful Analytical Concept
Authors: Trezzini Attilio 
Published: November 2020

By reconsidering the concept of normal degree of utilization in view of the fact that growth occurs through irregular fluctuations, the paper points out elements of weakness in the formalizations of the principles that underlie the notion of Harrodian instability. Since the latter was at the origin of the adoption of fully adjusted positions to represent long-term trends of the economy, such arguments call into question this way of addressing demand-led growth. JEL Classification: O40, E12