bluuebunny/crossref_metadata_2025_split huggingface dataset:
Validation of the Italian Version of the Revised Prenatal Coping Inventory (NuPCI) and its Correlations with Pregnancy-Specific Stress.
Authors: Penengo Chiara , Colli Chiara , Garzitto Marco , Driul Lorenza , Cesco Maddalena , Balestrieri Matteo 
Published: June 2020

Abstract
Background This cross-sectional study aimed to assess the psychometric properties of the Revised Prenatal Coping Inventory (NuPCI) in an Italian sample and to investigate how coping strategies were associated to pregnancy-specific stress.Methods Low-risk pregnant women (N = 211) were assessed with NuPCI, NuPDQ (Revised-Prenatal Distress Questionnaire), Brief-COPE (Coping Orientation to the Problems Experienced), and STAI (State-Trait Anxiety Inventory). The reliability of NuPCI was evaluated by assessing its internal consistency and factor structure. The concurrent validity between NuPCI and Brief-COPE and NuPDQ and STAI was investigated. Lastly, the relationship between NuPCI and NuPDQ was analyzed, as well as the ability of these scales to predict Apgar score at birth.Results Internal consistency of NuPCI scales was good for Planning-Preparation (ɑC=0.84) and Spiritual-Positive Coping (ɑC=0.81), acceptable for Avoidance (ɑC=0.76). The original three-factor structure was confirmed, using a promax solution. NuPCI and NuPDQ showed good concurrent validity. The NuPCI scales predicted NuPDQ score (Avoidance and Planning-Preparation positively, Spiritual-Positive Coping negatively). Also, Avoidance and Spiritual-Positive Coping scales were associated respectively to increasing and to decreasing Apgar score, with the moderation of NuPDQ.Conclusions Italian NuPCI has sound psychometric properties and it is a useful coping measure. NuPDQ showed also a good validity.
Analyzing Trend of Tropical Cyclone Activity Along Odisha Coast, India
Authors: Mishra Manoranjan , Pattnaik Namita , Dash Manoj Kumar , Kattel Dambaru Ballab , Mishra Shailendra Kumar 
Published: June 2020

Abstract
A general perception among scientific community and policy makers of developing countries exists that intensity and frequency of tropical cyclones are increasing over time due to climate change and global warming. In the present study, we have tried to investigate the trend and variability of tropical cyclone landfall along the Odisha coast, India. The data pertaining to landfall of the tropical cyclones were collected from Indian Metrological Department and were further filtered using Gaussian filter. The results were plotted in graphical form. Correlation and linear regressions analyses were carried out. The results highlight that the frequency and intensity of tropical cyclone exhibit a decreasing trend. It can be thus be inferred that growing economic loss and loss of lives are due to growing exposure of unplanned population settlements and business investments to extreme events. Therefore socio-economic landscapes need to be adjusted to the landfall of cyclone than blaming natural forces for the loss of lives and properties. Findings of the present study may help policy makers devising future policies in coastal zones.
Orofacial Manifestations of Chronic Renal Failure at Ahmed Gasim Hospital and Dr. Salma Center for Kidney Diseases. Khartoum
Authors: Abouna Arwa Mohamed 
Published: June 2020

Abstract
Background: Chronic renal failure is defined as kidney damage, or a reduction in the glomerular filtration rate, for three or more months. Few studies were performed regarding the oral health status of chronic renal failure patients in Sudan.Objective: Study aims to investigate the pattern of orofacial manifestations in patients with chronic renal failure in Sudan and how the disease affecting their oral health. Methods: The present study is a hospital based study conducted at Dr. Salma Center for Treatment of Kidney diseases, University of Khartoum, Sudan and Department of Nephrology at Ahmed Gasim Hospital for Heart Surgery and Kidney Diseases –khartoum Bahri, Sudan, between January to June 2019. Two hundred and twenty six patients participated in the study. The frequency and pattern of orofacial manifestations among those patient in addition to presence of additional systemic diseases ,duration of dialysis and mean levels of urea ,creatinine and hemoglobin were all investigated. Results: Oral lesions were present in 103 out of 226 (45.6%) patients with chronic renal failure .Xerostomia was the most manifestation encountered [145 (64.2%)], followed by orofacial abcess , ulcers and candidiasis . Hypertention was the commonest systemic disease encountered .No significant difference was found regarding the orofacial manifestations with duration of dialysis ,urea ,hemoglobin and creatinine mean levels.Conclusions: Prevalence of orofacial manifestations was significantly high among CRF patients .Xerostomia was the main complaint recognized. The findings of the present study emphasize the importance of dental practitioners in monitoring the oral health of patients with chronic renal faliure, and the need for regular clinical examinations to ensure early diagnosis and management of patients.
Craniofacial Morphology/Phenotypes Influence on Mandibular Range of Movement in the Design of a Mandibular Advancement Device
Authors: Sanz Pedro Mayoral , Reyes Mariano Garcia , Torras Alex Bataller , Castillo JA Cabrera , Lagravere Manuel 
Published: June 2020

Abstract
Background. The mandibular opening path movements have different directions according to the craniofacial morphology of the patient but always downward and backward, therefore increasing the collapse of the upper airway. The aim of this work is to determine if there is a relationship between the craniofacial morphology and the mandibular movement to help understand the impact on the mandibular position. Methods. 52 students with full permanent dentition aged 19 to 23 years (mean 21.3 SD 1.7; 29 females and 23 males), participated in the study. Each subject had a lateral cephalometric radiograph taken. The opening angle was determined for two levels of vertical openings at 5 and 10 mm.Results. The opening angle showed a greater variability between subjects ranging from 63,15 to 77,08 for 5 mm angle and from for 61,65 to 75,72 for the 10 mm angle. Differences of facial phenotypes was evident when comparing the individual dissoccluding angle of the low angle horizontal pattern and high angle vertical pattern.Conclusions. The opening angle is related to craniofacial morphology with higher vertical anterior and shorter anteroposterior faces having a more horizontal path of mandibular movement than shorter vertical anterior and longer anteroposterior subjects who have a more vertical path.
Diversity and Composition of Gut Microbiome of Cervical Cancer Patients by 16S rRNA and Whole-Metagenome Sequencing
Authors: Biegert Greyson , Karpinets Tatiana , Wu Xiaogang , Alam Molly B. El , Sims Travis T. , Yoshida-Court Kyoko , Lynn Erica J. , Yue Jingyan , Medrano Andrea Delgado , Petrosino Joseph , Mezzari Melissa P. , Ajami Nadim J. , Solley Travis , Ahmed-Kaddar Mustapha , Colbert Lauren E , Klopp Ann H. 
Published: June 2020

Abstract
Background Next generation sequencing has progressed rapidly, characterizing microbial communities beyond culture-based or biochemical techniques. 16S ribosomal RNA gene sequencing (16S) produces reliable taxonomic classifications and relative abundances, while shotgun metagenome sequencing (WMS) allows higher taxonomic and functional resolution at greater cost. The purpose of this study was to determine if 16S and WMS provide congruent information for our patient population from paired fecal microbiome samples. Results Comparative indices were highly congruent between 16S and WMS. The most abundant genera for 16S and WMS data did not overlap. Overlap was observed at the Phylum level, as expected. However, relative abundances correlated poorly between the two methodologies (all p>0.05). Hierarchical clustering of both sequencing analyses identified overlapping enterotypes. Both approaches were in agreement with regard to demographic variables.Conclusion Diversity, evenness and richness are comparable when using 16S and WMS techniques, however relative abundances of individual genera are not. Clinical associations with diversity and evenness metrics were similarly identified with WMS or 16S.
Clinical Design for Phase II/III Clinical Trials for Testing Therapeutic Interventions in COVID-19 Patients
Authors: Rai Shesh , Qian Chen , Pan Jianmin , Seth Anand , Srivast Deo Kumar , Bhatnagar Aruni 
Published: June 2020

Abstract
Background
Researchers around the world are urgently conducting clinical trials to develop new treatments for reducing mortality and morbidity related to COVID-19. However, due to unknown features of the disease and complexity of the patient population, traditional trial designs may not be optimal in such patients. We propose two independent clinical trials designs based on careful grouping of the expected characteristics of patient population. This could serve as a useful guide for researchers designing COVID-19 related Phase II/III trials.
Methods
Using the commonly utilized World Health Organization ordinal scale on patient status, we classify patients into three risk groups. In this approach, patients in Stages 3, 4 and 5 are categorized as the intermediate-risk group while patients in Stages 6 and 7 are categorized as the high-risk group. To ensure that an intervention, if deemed efficacious, is promptly made available to vulnerable patients, we propose a group sequential design with two interim analyses along with a final analysis and a toxicity monitoring rule for the intermediate-risk group. For the high-risk group, we propose a group sequential design with two interim analyses without toxicity monitoring.
Results
Based on different response rates, effect sizes, and power, required sample size and toxicity boundaries are calculated for each scenario. Sample size requirements for the designs with interim analyses are only marginally greater than the ones without. In addition, for both the intermediate-risk group and the high-risk group, conducting two interim analyses have identical required sample size compared with just one interim analysis. Additional issues that could potentially impact the trial are discussed.
Conclusions
We recommend using composite endpoints, with binary outcome for those in Stages 3, 4 and 5 with a power of 90% to detect an improvement of 20% in response rate, and 30 days mortality rate outcome for those in Stages 6 and 7 with a power of 90% to detect 15% (effect size) reduced mortality rate, in the COVID-19 trial design. For the intermediate-risk group, two interim analyses for efficacy evaluation along with toxicity monitoring are encouraged. For the high-risk group, two interim analyses without toxicity monitoring is advised.
Chronic Loss of Stag2 Leads to Altered Chromatin Structure Contributing to De-Regulated Transcription in Aml.
Authors: Smith James , Craig Stephanie G , Liberante Fabio G , Lappin Katrina M , Crean Clare M , McDade Simon , Thompson Alexander , Mills Kenneth , Savage Kienan 
Published: June 2020

Abstract
Background The cohesin complex plays a major role in folding the human genome into 3D structural domains. Mutations in members of the cohesin complex are known early drivers of myelodysplastic syndromes (MDS) and acute myeloid leukaemia (AML), with STAG2 the most frequently mutated complex member.Methods Here we use functional genomics (RNA-seq, ChIP-seq and HiChiIP) to investigate the impact of chronic STAG2 loss on three-dimensional genome structure and transcriptional programming in a clinically relevant model of chronic STAG2 loss.Results The chronic loss of STAG2 led to loss of smaller loop domains and the maintenance/formation of large domains that in turn led to altered genome compartmentalisation. These changes in genome structure lead to altered gene expression, including deregulation of the HOXA locus and the MAPK signalling pathway resulting in increased sensitivity to MEK inhibition.Conclusions The altered genomic architecture driven by the chronic loss of STAG2 results in altered gene expression that may contribute to leukaemogenesis which may be therapeutically targeted.
Factors associated with anemia among children 6-59 months in Ethiopia, using Ethiopia Demographic and Health Survey data, 2016
Authors: Woldegebreal Ataklity Gebretsadik , Gebrehiwot Gebremedhin Gebregziabiher , Desta Abraham Aregay , Ajemu Kiros Fenta , Berhe Asfawosen Aregay , Woldearegay Tewolde Wubayehu , Bezabih Nega Mamo 
Published: June 2020

Abstract
BackgroundAnemia is the most common nutritional problem and a wide spread micronutrient deficiency disorder even at global scale. In Ethiopia, child hood anemia is the most prevalent and a serious public health problem. The aim of this study is to assess determinants of anemia among children 6–59 months in Ethiopia, Ethiopia Demographic and Health Survey, 2016.MethodThis study was based on the 2016 Ethiopian Demographic Health Survey (EDHS).A community-based cross-sectional with a two-stage stratified cluster sampling technique was implemented at national level. Total of 7689 children were included in the analysis. Descriptive, bivariate and multivariable logistic regression analysis was employed.ResultThe prevalence of anemia among 6–59 months old children was 56.6% and highest among 6–11 months age groups. Child age 6–23 months, maternal age 20–29 and 30–39 years, poorest wealth index, mothers not currently living with husband, Birth order of the child eighth and, birth interval, one times, stunted children and underweight were significantly associated with anemiaConclusionsThe prevalence of anemia in this study was the highest from all EDHS reports. Itwas skyrocketed from the preceding report (EDHS 2011) and remains the main public health problem in Ethiopia. Comprehensive intervention strategies should be in place tailored to different government hierarchies (national, regional and district level) including household and individual level interventions for combating child hood anemia by giving an emphasis on the identified variables.
Expansion or Incubation? Which is Better to Manipulating Stem Cells for Improving the Effects of Umbilical Cord Blood Transplantation
Authors: Lei Yang-Yang , Wang Lu , Deng Lei , Yang Yang , Zhang Ang , Chang Shao-Hong , Hua Wen-Ming , Rong Bin , Guo Mei , Ai Hui-Sheng , Hu Kai-Xun 
Published: June 2020

Abstract
Background: Delayed hematopoietic recovery was one of the factors that limited the clinic application of umbilical cord blood (UCB) transplantation. Two different methods of overcoming the limitation were evaluated by this systematic review and meta-analysis. One strategy was to expand UCB unit ex vivo for increasing cell dose and another strategy was to incubate UCB unit with small molecules in vitro for enhancing bone marrow (BM) homing ability. Methods: Relevant literatures were collected from 2000 to 2020 by searching the databases of PubMed, Medline, and EMBASE. A total of 15 studies that met the inclusion criteria were included in the meta-analysis. Results: Similar clinical effects including neutrophil recovery (P=0.308), platelet recovery (P=0.4754), and 100-day survival (P=0.4216) between two methods were observed. Faster neutrophil recovery was associated with higher infused total nucleated cells (TNCs) (R2=0.4526 P<0.05) and higher infused CD34+ cells (R2=0.9777 P=0.095) in patients of expansion group and incubation group, respectively. Conclusion: Although the treatment outcomes by using expanded stem cells were getting better all these years with fast development of cell culture techniques according to our cumulative meta-analysis. It seems the application of incubation procedure was safer and more convenient than expansion regarding to their similar clinic effects. This study was registered at RROSPERO (Registration no. CRD42020149750).
CircRNA CDR1as Promotes Glioma Progression by Regulating miR-514a-3p/STAT3 Signaling Pathway
Authors: Cao Yiqun , Yang Wentao , Li Deheng , Li Liangdong , Chen Xin , Chen Lei , Hao Bin , IW Lao , Wang Qifeng 
Published: June 2020

Abstract
Background The pathogenesis of glioma is very complicated and the molecular mechanisms have not been clearly demonstrated so far. CircRNA CDR1as as non-coding RNA was highly expressed in multiple human cancers and promoted tumorigenesis and cancer progression. However, there were no reports to address the roles of CDR1as in glioma.Methods In this study, we used glioma cell lines to investigate the function of CDR1as. Cell viability, colony formation, migration, invasion, gene expression, luciferase activity, miRNA-circRNA interaction, protein-circRNA interaction, and tumor formation in vivo were assessed.Results We found that CDR1as is up-regulated in human glioma tissues and cell lines, and its expression is significantly associated with the prognosis of patients with glioma. Loss-of-function studies revealed that CDR1as increases glioma cell proliferation, migration, and invasion in vitro and tumor growth in vivo. We showed that CDR1as can bind miR-514a-3p and act as a sponge of miR-514a-3p. Furthermore, we identified that the expression of STAT3 is regulated by miR-514a-3p and CDR1as as the direct target of miR-514a-3p. Additionally, the decreased glioma cell malignancy by CDR1as knockdown can be reversed by miR-514a-3p inhibitor and overexpression of STAT3. CDR1as knockdown can result in the down-regulated expression of PCNA, Ki67, N-cadherin, and MMP9 and up-regulated expression of E-cadherin. Moreover, the aberrant expression of these genes by CDR1as knockdown also can be reversed by miR-514a-3p inhibitor and overexpression of STAT3.Conclusions Taken together, our findings uncover the molecular mechanisms of CDR1as-mediated the progression of glioma through the miR-514a-3p/STAT3 signaling pathway. These results will provide a theoretical basis for further understanding of the molecular mechanisms of glioma tumorigenesis and developing new therapeutic targets.
Association Between Micronutrient Dietary Intake and Semen Quality: A Cross-Sectional Study
Authors: Bazarganipour Fatemeh , Akbarzadeh Gouhar , Asemi Zatollah , Kutenaee Maryam Azizi , Sadeghi Hossein , Khashavi Zahra , Taghavi Seyed Abdolvahab 
Published: June 2020

Abstract
Background: To evaluate association between sperm parameters and micronutrient dietary intake in young Iranian men.Material and methods: This cross-sectional study includes 520 men referred to the Infertility Clinic. The quality of semen was measured by measuring the concentration, number, motility and morphology according to WHO guidelines. The micronutrient content of the diet was assessed using a valid food frequency questionnaire (FFQ).Results: After age and BMI adjustment, increased intake of saturated fatty acids, and decreased intake of linoleic acids and selenium were associated with count of sperm (P<0.05). Decreased dietary intake of selenium, zinc and vitamin E were also associated with sperm concentrations (P<0.05). In addition, zinc, vitamin E, selenium and vitamin D were associated with motility of sperm (P<0.05). Increased intake of saturated fatty acids, and decreased intake of linoleic acids and zinc were associated with morphology of sperm (P<0.05).Conclusions: In summary, we found a significant unfavorable association between sperm quality and increased dietary intake of saturated fatty acids, decreased intake of linoleic acids; selenium, zinc, vitamin E and vitamin D. Diets containing higher micronutrient antioxidants and lower saturated fats were associated with optimal sperm quality parameters.
Assessment of urinary biomarkers of mycotoxin exposure in adults from Cameroon
Authors: Abia Wilfred Angie , Tchana Angele N. , Djonabaye Doumani , Šarkanj Bojan , Mfopou Euloge Yiagnigni , Ezekiel Chibundu N. , Sulyok Micheal , Turner Paul C. , Elliott Christopher T. , Warth Benedikt , Krska Rudolf , Moundipa Paul F. 
Published: June 2020

Abstract
Background

In Cameroon dietary staples are contaminated with diverse toxic fungal metabolites, known as mycotoxins. Aflatoxins and fumonisins are of particular public health concern, particularly in relation to cancer and/or early life stunting. Mixtures of these toxins are predicted from food measures, and in this work, the levels and frequencies of urinary mycotoxin biomarkers are reported in Cameroonian adults.
Methods

A single first void urine sample was collected from 89 adults (aged range: 28–85, male 39, female 50) from the city of Yaoundé, Centre Region, Cameroon. Urines were tested for eight distinct mycotoxins using measures of both parent compounds and/or their metabolites by Liquid Chromatography tandem mass spectrometry (LC-MS/MS).
Results

Altogether seven distinct mycotoxins, aflatoxin, fumonisin, deoxynivalenol, zearalenone, nivalenol, ochratoxin A and citrinin, (or their metabolites) were observed in urine samples. At least one mycotoxin was detected in all of the urine samples, 87 (97.8%) of which were above the method’s quantification limit. Aflatoxin M1 was detected in 42% (n.d. − 0.21 µg L− 1) of samples of which about a quarter additionally contained fumonisin B1. Of the remaining toxins deoxynivalenol, zearalenone, ochratoxin A, nivalenol and citrinin were present in 78%, 99% 95%, 53%, and 87% of the samples respectively. Alternariol was not detected in any sample. Mixtures of mycotoxins in the samples were frequently observed with 64 samples (72%) containing more than five mycotoxins. Estimates of intake exceeded the TDIs for fumonisin (n = 4), deoxynivalenol (n = 1) and zearalenone (n = 2), no TDI is set for aflatoxin.
Conclusions

This study reveals frequent co-exposure of Cameroonian individuals to a complex mixture of toxic and carcinogenic mycotoxins, with mixtures of aflatoxin and fumonisin a particular priority from a public health standpoint.