bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ferroptosis contributes to developmental cell death in rice blast
Authors: Shen Qing , Liang Meiling , Yang Fan , Deng Yi Zhen , Naqvi Naweed I. 
Published: November 2019

AbstractWe identified that ferroptosis, an iron-dependent non-apoptotic cell death process, occurs in the rice blast fungusMagnaporthe oryzae, and plays a key role in infection-related development therein. Ferroptosis in the blast fungus was confirmed based on the four basic criteria. We confirmed the dependence of ferroptosis on ferric ions, and optimized C11-BODIPY581/591as a key sensor for subcellular detection and quantification of lipid peroxides that mediate ferroptotic cell death during the pathogenic growth phase ofM. oryzae.In addition, we uncovered an important regulatory function for reduced glutathione and the NADPH oxidases in generating/modulating the superoxide moieties for ferroptotic cell death inMagnaporthe. Ferroptosis was found to be necessary for the specific developmental cell death in conidia during appressorium maturation in rice blast. Such ferroptotic cell death initiated first in the terminal cell and progressed sequentially to the entire conidium. Chelation of iron or chemical inhibition of ferroptosis caused conidial cells to remain viable and led to strong defects in host invasion byM. oryzae.Precocious induction of ferroptosis in a blast-susceptible rice cultivar led to resistance againstM. oryzaeinvasion. Interestingly, ferroptosis and autophagy were found to play inter-reliant or codependent roles in contributing to such precise cell death inM. oryzaeconidia during pathogenic differentiation. Our study provides significant molecular insights into understanding the role of developmental cell death and iron homeostasis in infection-associated morphogenesis and in fungus-plant interaction in the blast pathosystem.
Proprioception is subject-specific and improved without performance feedback
Authors: Wang Tianhe , Zhu Ziyan , Kana Inoue , Yu Yuanzheng , He Hao , Wei Kunlin 
Published: November 2019

AbstractAccumulating evidence indicates that the human’s proprioception map appears subject-specific. However, whether the idiosyncratic pattern persists across time with good within-subject consistency has not been quantitatively examined. Here we measured the proprioception by a hand visual-matching task in multiple sessions over two days. We found that people improved their proprioception when tested repetitively without performance feedback. Importantly, despite the reduction of average error, the spatial pattern of proprioception errors remained idiosyncratic. Based on individuals’ proprioceptive performance, a standard convolutional neural network classifier could identify people with good accuracy. We also found that subjects’ baseline proprioceptive performance could not predict their motor performance in a visual trajectory-matching task even though both tasks require accurate mapping of hand position to visual targets in the same workspace. Using a separate experiment, we not only replicated these findings but also ruled out the possibility that performance feedback during a few familiarization trials caused the observed improvement in proprioception. We conclude that the conventional proprioception test itself, even without feedback, can improve proprioception but leave the idiosyncrasy of proprioception unchanged.
Switch from translation initiation to elongation needs Not4 and Not5 collaboration
Authors: Allen George E , Panasenko Olesya O , Villanyi Zoltan , Zagatti Marina , Weiss Benjamin , Polte Christine , Ignatova Zoya , Collart Martine A 
Published: November 2019

AbstractNot4 and Not5 are crucial components of the Ccr4-Not complex with pivotal functions in mRNA metabolism. Both associate with ribosomes but mechanistic insights on their function remain elusive. Here we determine that Not5 and Not4 synchronously impact translation initiation and Not5 alone alters translation elongation. Deletion of Not5 causes elongation defects in a codon-dependent fashion, increasing and decreasing the ribosome dwelling occupancy at minor and major codons, respectively. This larger difference in codons’ translation velocities alters translation globally and enables kinetically unfavorable processes such as nascent chain deubiquitination to take place. In turn, this leads to abortive translation and favors protein aggregation. These findings highlight the global impact of Not4 and Not5 in controlling the speed of mRNA translation and transition from initiation to elongation.SummaryNot4 and Not5 regulate translation synchronously but distinguishably, facilitating smooth transition from initiation to elongation
EMIn-depth profiling of calcite precipitation by environmental bacteria reveals fundamental mechanistic differences with relevance to application
Authors: Reeksting Bianca J. , Hoffmann Timothy D. , Tan Linzhen , Paine Kevin , Gebhard Susanne 
Published: November 2019

SUMMARYMicrobial-induced calcite precipitation (MICP) has not only helped to shape our planet’s geological features, but is also a promising technology to address environmental concerns in civil engineering applications. However, limited understanding of the biomineralization capacity of environmental bacteria impedes application. We therefore surveyed the environment for different mechanisms of precipitation across bacteria. The most fundamental difference was ureolytic ability, where urease-positive bacteria caused rapid, widespread increases in pH, while non-ureolytic strains produced such changes slowly and locally. These pH shifts correlated well with patterns of precipitation on solid media. Strikingly, while both mechanisms led to high levels of precipitation, we observed clear differences in the precipitate. Ureolytic bacteria produced homogenous, inorganic fine crystals, whereas the crystals of non-ureolytic strains were larger with a mixed organic/inorganic composition. When representative strains were tested in application for crack healing in cement mortars, non-ureolytic bacteria gave robust results, while ureolytic strains showed more variation. This may be explained by our observation that urease activity varied between growth conditions, or by the different nature and therefore material performance of the precipitate. Our results shed light on the breadth of biomineralization activity among environmental bacteria, an important step towards the rational design of bacteria-based engineering solutions.
Multiplexed biosensors for precision bacteria tropism in vivo
Authors: Chien Tiffany , Harimoto Tetsuhiro , Kepecs Benjamin , Gray Kelsey , Coker Courtney , Pu Kelly , Azad Tamjeed , Danino Tal 
Published: November 2019

AbstractThe engineering of microbes spurs biotechnological innovations, but requires control mechanisms to confine growth within defined environments for translation. Here we engineer bacterial growth tropism to sense and grow in response to specified oxygen, pH, and lactate signatures. Coupling biosensors to drive essential gene expression reveals engineered bacterial localization within upper or lower gastrointestinal tract. Multiplexing biosensors in an AND logic-gate architecture reduced bacterial off-target colonization in vivo.
A thermophilic phage uses a small terminase protein with a fixed helix-turn-helix geometry
Authors: Hayes Janelle A. , Hilbert Brendan J. , Gaubitz Christl , Stone Nicholas P. , Kelch Brian A. 
Published: November 2019

SUMMARYTailed bacteriophage use a DNA packaging motor to encapsulate their genome during viral particle assembly. The small terminase (TerS) component acts as a molecular matchmaker by recognizing the viral genome as well as the main motor component, the large terminase (TerL). How TerS binds DNA and the TerL protein remains unclear. Here, we identify the TerS protein of the thermophilic bacteriophage P74-26. TerSP76-26 oligomerizes into a nonamer that binds DNA, stimulates TerL ATPase activity, and inhibits TerL nuclease activity. Our cryo-EM structure shows that TerSP76-26 forms a ring with a wide central pore and radially arrayed helix-turn-helix (HTH) domains. These HTH domains, which are thought to bind DNA by wrapping the helix around the ring, are rigidly held in an orientation distinct from that seen in other TerS proteins. This rigid arrangement of the putative DNA binding domain imposes strong constraints on how TerSP76-26 can bind DNA. Finally, the TerSP76-26 structure lacks the conserved C-terminal β-barrel domain used by other TerS proteins for binding TerL, suggesting that a well-ordered C-terminal β-barrel domain is not necessary for TerS to carry out its function as a matchmaker.
High-efficiency multi-site genomic editing (HEMSE) ofPseudomonas putidathrough thermoinducible ssDNA recombineering
Authors: Aparicio Tomas , Nyerges Akos , Martínez-García Esteban , de Lorenzo Víctor 
Published: November 2019

SUMMARYWhile single-stranded DNA recombineering is a powerful strategy for higher-scale genome editing, its application to species other than enterobacteria is typically limited by the efficiency of the recombinase and the action of native mismatch repair (MMR) systems. By building on [i] availability of the Erf-like single-stranded DNA-annealing protein Rec2, [ii] adoption of tightly-regulated thermoinducible device and [iii] transient expression of a MMR-supressingmutLallele, we have set up a coherent genetic platform for entering multiple changes in the chromosome ofPseudomononas putidawith an unprecedented efficacy and reliability. The key genetic construct to this end is a broad host range plasmid encoding co-transcription ofrec2andP. putida’smutLE36KPPat high levels under the control of the PL/cI857 system. Cycles of short thermal shifts ofP. putidacells followed by transformation with a suite of mutagenic oligos delivered different types of high-fidelity genomic changes at frequencies up to 10% per single change—that can be handled without selection. The same approach was instrumental to super-diversify short chromosomal portions for creating libraries of functional genomic segments—as shown in this case with ribosomal binding sites. These results enable the multiplexing of genome engineering ofP. putida, as required for metabolic engineering of this important biotechnological chassis.
Multi-functional antibodies are induced by the RTS,S malaria vaccine and associated with protection in a phase I/IIa trial
Authors: Kurtovic Liriye , Atre Tanmaya , Feng Gaoqian , Wines Bruce D. , Chan Jo-Anne , Boyle Michelle J. , Drew Damien R. , Mark Hogarth P. , Fowkes Freya J. I. , Bergmann-Leitner Elke S. , Beeson James G. 
Published: November 2019

ABSTRACTBackgroundRTS,S is the leading malaria vaccine candidate, but only confers partial efficacy against malaria in children. RTS,S is based on the majorPlasmodium falciparumsporozoite surface antigen, circumsporozoite protein (CSP). The induction of anti-CSP antibodies is important for protection, however, it is unclear how protective antibodies function.MethodsWe quantified the induction of functional anti-CSP antibody responses in healthy malaria-naïve adults (N=45) vaccinated with RTS,S/AS01. This included the ability to mediate effector functions via the fragment crystallizable (Fc) region, such as interacting with human complement proteins and Fcγ-receptors (FcγRs) that are expressed on immune cells, which promote various immunological functions.ResultsOur major findings were i) RTS,S-induced antibodies mediate Fc-dependent effector functions, ii) functional antibodies were generally highest after the second vaccine dose; iii) functional antibodies targeted multiple regions of CSP, iv) participants with higher levels of functional antibodies had a reduced probability of developing parasitemia following homologous challenge (p<0.05); v) non-protected subjects had higher levels of anti-CSP IgM.ConclusionsOur data suggests a role for Fc-dependent antibody effector functions in RTS,S-induced immunity. Enhancing the induction of these functional activities may be a strategy to improve the protective efficacy of RTS,S or other malaria vaccines.
Protein products of non-stop mRNA disrupt nucleolar homeostasis
Authors: Davis Zoe H. , Mediani Laura , Vinet Jonathan , Alberti Simon , Holehouse Alex S. , Carra Serena , Brandman Onn 
Published: November 2019

SummaryMutations that cause ribosome stalling or impair the cell’s protective response to stalling have been demonstrated to cause neurodegeneration, yet the mechanisms underlying these pathologies remain poorly understood. Here we investigated the fate of defective proteins translated from stall-inducing, nonstop mRNA that escape ubiquitylation by the Ribosome-associated Quality Control (RQC) E3 ligase LTN1. We found that nonstop protein products accumulated in nucleoli and this localization was driven by polylysine tracts produced by translation of the poly(A) tail of nonstop mRNA. Nucleolar sequestration increased the solubility of invading proteins but disrupted nucleoli, altering their dynamics, morphology, and resistance to stress. Changes in nucleolar morphology are consistent with a simple physical model in which LTN1 impairment enhances the inter-molecular interactions of nucleolar components. Our work elucidates how failure to degrade the protein products of stalled translation may affect distal cellular processes and will inform studies on the pathology of neurodegenerative disease.
ML2S-SVM: multi-label least-squares support vector machine classifiers
Authors: Xu Shuo , An Xin 
Published: November 2019

Purpose
Image classification is becoming a supporting technology in several image-processing tasks. Due to rich semantic information contained in the images, it is very popular for an image to have several labels or tags. This paper aims to develop a novel multi-label classification approach with superior performance.

Design/methodology/approach
Many multi-label classification problems share two main characteristics: label correlations and label imbalance. However, most of current methods are devoted to either model label relationship or to only deal with unbalanced problem with traditional single-label methods. In this paper, multi-label classification problem is regarded as an unbalanced multi-task learning problem. Multi-task least-squares support vector machine (MTLS-SVM) is generalized for this problem, renamed as multi-label LS-SVM (ML2S-SVM).

Findings
Experimental results on the emotions, scene, yeast and bibtex data sets indicate that the ML2S-SVM is competitive with respect to the state-of-the-art methods in terms of Hamming loss and instance-based F1 score. The values of resulting parameters largely influence the performance of ML2S-SVM, so it is necessary for users to identify proper parameters in advance.

Originality/value
On the basis of MTLS-SVM, a novel multi-label classification approach, ML2S-SVM, is put forward. This method can overcome the unbalanced problem but also explicitly models arbitrary order correlations among labels by allowing multiple labels to share a subspace. In addition, the multi-label classification approach has a wider range of applications. That is to say, it is not limited to the field of image classification.
Supply chains under resource pressure
Authors: Kalaitzi Dimitra , Matopoulos Aristides , Bourlakis Michael , Tate Wendy 
Published: November 2019

PurposeThe purpose of this paper is to investigate the implications of supply chain strategies that manufacturing companies can use to minimise or overcome natural resource scarcity, and ultimately improve resource efficiency and achieve competitive advantage. The relationship between resource efficiency and competitive advantage is also explored.Design/methodology/approachThe proposed research model draws on resource dependence theory. Data were collected from 183 logistics, purchasing, sustainability and supply chain managers from various manufacturing companies and analysed by applying the partial least squares structural equation modelling technique.FindingsThe results indicate that both buffering and bridging strategies improve resource efficiency; however, only bridging strategies seem to lead to firm’s competitive advantage in terms of ownership and accessibility to resources. The relationship between resource efficiency and competitive advantage is not supported.Research limitations/implicationsFuture research could confirm the robustness of these findings by using a larger sample size and taking into account other supply chain members.Practical implicationsThis research provides guidance to managers faced with the growing risk of resource scarcity to achieve a resource efficient supply chain and an advantage over competitors.Originality/valueStudies have explored the appropriate strategies for minimising dependencies caused by the scarcity of natural resources in the field of supply chain management; however, there is limited empirical work on investigating the impact of these strategies on resource efficiency and competitive advantage.
Do recovery processes need empowered frontline employees?
Authors: Santos Juliana Bonomi , Hernandez José Mauro , Leão Wandick 
Published: November 2019

PurposeThe purpose of this paper is to investigate whether frontline employee empowerment (FEE) is necessary in the presence of streamlined recovery processes when customers attribute responsibility for the recovery process to the service provider.Design/methodology/approachThe hypotheses were tested through a survey conducted with 253 bank customers, combined with two laboratory experiments run with 354 undergraduate students to assess service recovery efforts by an online store and a clinical laboratory.FindingsCustomers who attribute more responsibility for the recovery process to service providers only become more satisfied with FEE when recovery processes are not streamlined. The presence of streamlined processes and FEE is not sufficient to raise post-recovery satisfaction levels in individuals who attribute little responsibility for the process to service providers.Originality/valueThe study extends the literature on contingencies that influence the design of recovery strategies by showing when FEE matters. It also highlights the risks of designing service recovery practices, such as FEE or streamlined recovery processes, without considering that different customers do not evaluate such efforts in the same fashion. Research on service recovery design needs to fully integrate concepts from marketing, operations and human resources when the goal is to evaluate the effectiveness of such practices. The outcomes also offer managers insights for designing recovery strategies.
The magnificent “I” in business education: evidence from Greece
Authors: Anninos Loukas N. , Chytiri Alexandra Paraskevi , Chytiris Leonidas 
Published: November 2019

Purpose
The purpose of this paper is twofold: first, to examine the level of narcissism and its individual traits in students who study business, in the particular context of a regional country such as Greece; and, second, to test how several demographic variables are related to narcissism levels.

Design/methodology/approach
The study consists of a theoretical part on narcissism in business education and an empirical part that was based on a survey conducted with the use of a questionnaire. The analysis includes hypothesis testing and basic statistical tests.

Findings
Findings suggest that sex, study levels, years of business experience and (personal/family) income do impact specific narcissistic dimensions, which may be a cause for concern both for employers and higher education providers.

Research limitations/implications
The study was conducted in a regional country, the participants were students of public higher education institutions only and the questionnaire was self-reported, which could lead to likely social desirability effects.

Practical implications
The investigation of narcissism in the Greek business education might be of interest to business education providers (for providing curriculum that help future managers/leaders to deploy the positive characteristics of narcissism and avoid or not to develop the negative ones) and to future employers to apply more effective human resource practices, i.e. selection, training, rewarding.

Originality/value
The study at hand aimed to investigate the presence of narcissism and its individual (narcissistic) behavioral dimensions in students studying business in Greece.
Impact of ethnicity and self-employment on initial financing and business performance in entrepreneurship in China
Authors: Appiah Bismark Kusi , Donghui Zhang , Majumder Shapan Chandra 
Published: November 2019

Purpose
The purpose of the current study is to analyze the influence that is caused by ethnicity and volunteer self-employment in entrepreneurship on the initial financing and business performance of Chinese enterprises. The increasing trend of entrepreneurship has raised certain ethnic challenges in entrepreneurship owing to globalization and now there are several minority groups that are running their households in China. So, the current study aims to assess whether the minority-operated and majority-operated firms have different access to initial financing and different business performance.

Design/methodology/approach
The data are collected from 25 Chinese enterprises about the current variables, and the relationships are tested. This study has used the ordinary least of square (OLS) regression model to examine the findings.

Findings
The purposive sampling is used in the current study because the purpose of the present research is to understand and study the role of ethnicity and self-employment in initial financing and business performance of Chinese enterprises and so the data was collected from related enterprises. This study has used the ordinary least of square (OLS) regression model to examine the findings.

Originality/value
The current findings have significant implications in theory and practice. This study will be a great addition to the literature because the self-employment has never been examined before in such models to assess the role of forceful or volunteer self-employment in entrepreneurship, and so, it will open new doors of research for future researchers.
Wine and wine tourism experience: a theoretical and conceptual review
Authors: Santos Vasco Ribeiro , Ramos Paulo , Almeida Nuno , Santos-Pavón Enrique 
Published: November 2019

Purpose
This paper aims to provide a theoretical and conceptual analysis of wine and wine tourism experiences evidencing the current state of the art and providing some directions for future research.

Design/methodology/approach
The paper provides an in-depth literature review and content analysis of prior work.

Findings
The experience focussed on wine andwine tourismrequires further exploration. The boundaries of the wine andwine tourism experience were identified, togetherwith highlights and strategic agenda for future actions.

Originality/value
Based on some key prior literature on the topic of wine and tourism experience, future research directions and approaches were proposed.