bluuebunny/crossref_metadata_2025_split huggingface dataset:
Examining the use of Cannabinoid-based Medicine among older residential care recipients diagnosed with dementia: A study protocol for a double blind randomised cross-over trial
Authors: Timler Amanda , Bulsara Caroline , Bulsara Max , Vickery Alistair , Smith Jill , Codde Jim 
Published: November 2019

Abstract
Background: Dementia is a neurological condition that affects the cognitive and functional ability of the brain and is the leading cause of disability among those aged 65-years and above. More effective ways to manage dementia symptoms are needed as current treatment options (anti-depressant and antipsychotic) can be ineffective and associated with substantial side-effects, including increased rate of mortality. Cannabinoid-based medicines (CBM) has shown to inhibit some symptoms associated with dementia and the adverse effects are often minimal, yet little research has explored the use of CBM among this population. Aim. To monitor the safety of a purified dose of CBM oil (3:2 THC:CBD) on behaviour symptoms, Quality of life (QOL), and discomfort caused by pain. Methods/Design: We will carry out an 18-week randomised, double-blinded, crossover trial that consists of a 2-week eligibility period, two, 6-week treatment cycles and two, 2-week washout periods (between both cycles, and after the second treatment cycle). We aim to recruit 50 participants with dementia who are living in residential aged-care facilities. The participants will be randomised into two groups, and receive a dose of either CBM oil or placebo for the first treatment cycle and receive the opposite medication for the second. The Neuropsychiatric Inventory Questionnaire, Cohen Mansfield Agitation Inventor, Quality of Life-Alzheimer’s Disease questionnaire, and the Abbey Pain Scale will be collected on seven occasions, and completed by the participants, aged-care staff, and nominated next of kin or family member. The participant’s heart rate and blood pressure will be monitored weekly and their body composition and weight will be monitored fortnightly by a research nurse to assess individual dose response and frailty. In addition, pre- and post-surveys will be administered to aged-care staff and family members to understand their perceptions towards CBM, and to inform proposed focus groups consisting of the aged care staff and next of kin. Discussion: The study design has been informed by medical professionals and key stakeholders including those working in the residential aged-care industry to ensure patient safety, collection of non-invasive measures, and methodological rigor and study feasibility. Trial registration: ACTRN12619000474156 Registered 21 March 2019, https://www.anzctr.org.au/Trial/Registration/TrialReview.aspx?id=375128&isClinicalTrial=False
Separation of the Epitopes in a Multi-Epitope Chimera: Helical or Flexible Linkers
Authors: Kabiri Mona , Tafaghodi Mohsen , Saberi Mohammad Reza , Moghadam Maliheh , Rezaee Seyed Abdolrahim , Sankian Mojtaba 
Published: November 2019

Background:
The engineered chimeric peptides including functional multi-epitope
structures fused by various peptide linkers are widely applied in biotechnological research to
improve the expression level and biological activity of chimera.

Objective:
The aim of our study was to evaluate the effect of helical and flexible linkers on
solubility, expression level and folding of multi-epitope chimera containing four epitopes of Human
T Lymphotropic Virus Type 1 (HTLV-1).

Methods:
For this purpose, the chimera sequences connected by the helical or flexible linker were
inserted into different plasmid vectors and expressed in E. coli strains. The expressed products were
analyzed using SDS-PAGE and Western blot techniques. Additionally, the molecular modeling
study of the chimera with helical or flexible linker was performed using iterative threading
assembly refinement (I-TASSER) to attain their three-dimensional structures.

Results:
Comparison of the chimera expression indicated that the insertion of a flexible
(GGGGS)3 linker among chimera epitopes could significantly enhance the level of expression,
whereas, the low-level of chimera expression was observed for chimera containing the contiguous
helical (EAAAK)5 linker. According to the results of sequence alignment and plasmid stability test,
the structure and function of a consecutive helical linker among chimera epitopes were similar to
porins as the outer-membrane pore-forming proteins. The molecular modeling results confirmed
our experimental study.

Conclusion:
This investigation illustrated the key role of linker design in determining the
expression level of multi-epitope chimera and conformational folding.
Antibiotics Application Strategies to Control Biofilm Formation in Pathogenic Bacteria
Authors: Khan Fazlurrahman , Pham Dung T.N. , Oloketuyi Sandra F. , Kim Young-Mog 
Published: November 2019

Background:The establishment of a biofilm by most pathogenic bacteria has been known as one of the resistance mechanisms against antibiotics. A biofilm is a structural component where the bacterial community adheres to the biotic or abiotic surfaces by the help of Extracellular Polymeric Substances (EPS) produced by bacterial cells. The biofilm matrix possesses the ability to resist several adverse environmental factors, including the effect of antibiotics. Therefore, the resistance of bacterial biofilm-forming cells could be increased up to 1000 times than the planktonic cells, hence requiring a significantly high concentration of antibiotics for treatment.Methods:Up to the present, several methodologies employing antibiotics as an anti-biofilm, antivirulence or quorum quenching agent have been developed for biofilm inhibition and eradication of a pre-formed mature biofilm.Results:Among the anti-biofilm strategies being tested, the sub-minimal inhibitory concentration of several antibiotics either alone or in combination has been shown to inhibit biofilm formation and down-regulate the production of virulence factors. The combinatorial strategies include (1) combination of multiple antibiotics, (2) combination of antibiotics with non-antibiotic agents and (3) loading of antibiotics onto a carrier.Conclusion:The present review paper describes the role of several antibiotics as biofilm inhibitors and also the alternative strategies adopted for applications in eradicating and inhibiting the formation of biofilm by pathogenic bacteria.
Prediction of Epitope-Based Peptide Vaccine Against the Chikungunya Virus by Immuno-informatics Approach
Authors: Anwar Saeed , Mourosi Jarin T. , Khan Md. Fahim , Hosen Mohammad J. 
Published: November 2019

Background:
Chikungunya is an arthropod-borne viral disease characterized by abrupt onset
of fever frequently accompanied by joint pain, which has been identified in over 60 countries in Africa,
the Americas, Asia, and Europe.

Methods:
Regardless of the availability of molecular knowledge of this virus, no definite vaccine or
other remedial agents have been developed yet. In the present study, a combination of B-cell and T-cell
epitope predictions, followed by molecular docking simulation approach has been carried out to design
a potential epitope-based peptide vaccine, which can trigger a critical immune response against the viral
infections.

Results:
A total of 52 sequences of E1 glycoprotein from the previously reported isolates of
Chikungunya outbreaks were retrieved and examined through in silico methods to identify a potential
B-cell and T-cell epitope. From the two separate epitope prediction servers, five potential B-cell
epitopes were selected, among them “NTQLSEAHVEKS” was found highly conserved across strains
and manifests high antigenicity with surface accessibility, flexibility, and hydrophilicity. Similarly,
two highly conserved, non-allergenic, non-cytotoxic putative T-cell epitopes having maximum population
coverage were screened to bind with the HLA-C 12*03 molecule. Molecular docking simulation
revealed potential T-cell based epitope “KTEFASAYR” as a vaccine candidate for this virus.

Conclusion:
A combination of these B-cell and T-cell epitope-based vaccine can open up a new skyline
with broader therapeutic application against Chikungunya virus with further experimental and
clinical investigation.
Ligand-Based Pharmacophore Modeling and Virtual Screening to Discover Novel CYP1A1 Inhibitors
Authors: Tahir Rana Adnan , Hassan Farwa , Kareem Abdul , Iftikhar Umer , Sehgal Sheikh Arslan 
Published: November 2019

Background:Cytochrome P450, family 1, subfamily A, polypeptide 1 (CYP1A1) is an imperative enzyme due to its immersion in the biotransformation of a wide range of drugs and other xenobiotics. The involvement of enzymes in drug metabolism indicates an effective drug target for the development of novel therapeutics. The discovery of CYP1A1 specific inhibitors would be of particular relevance for the clinical pharmacology.Method:In the current work, in silico approaches were utilized to identify the novel potential compounds through a diverse set of reported inhibitors against CYP1A1. A dataset of reported compounds against CYP1 belongs to 10 different classes (alkaloids, coumarins, flavonoids, natural compounds, synthetic inhibitors, drugs, MBI’s, PAHs, naphthoquinone and stilbenoids) was retrieved and utilized for the comparative molecular docking analyses followed by pharmacophore modeling. The total eleven novel compounds were scrutinized on the basis of the highest binding affinities and least binding energy values.Result:ZINC08792486 compound attained the highest gold fitness score of 90.11 against CYP1A1 among all the scrutinized molecules.Conclusion:It has been elucidated that the residues Phe-224, Gly-316 and Ala-317 were conserved in all ligand-receptor interactions and critical for the development of effective therapies. The ADMET property analyses also predict better absorption and distribution of the selected hits that may be used in the future for in vitro validations and drug development.
Anticancer Activity of Platinum (II) Complex with 2-Benzoylpyridine by Induction of DNA Damage, S-Phase Arrest, and Apoptosis
Authors: Li Yu-Lan , Gan Xin-Li , Zhu Rong-Ping , Wang Xuehong , Liao Duan-Fang , Jin Junfei , Huang Zhaoquan 
Published: November 2019

Objective:
To overcome the disadvantages of cisplatin, numerous platinum (Pt) complexes have been
prepared. However, the anticancer activity and mechanism of Pt(II) complexed with 2-benzoylpyridine [Pt(II)-
Bpy]: [PtCl2(DMSO)L] (DMSO = dimethyl sulfoxide, L = 2-benzoylpyridine) in cancer cells remain unknown.

Methods:
Pt(II)-Bpy was synthesized and characterized by spectrum analysis. Its anticancer activity and underlying
mechanisms were demonstrated at the cellular, molecular, and in vivo levels.

Results:
Pt(II)-Bpy inhibited tumor cell growth, especially HepG2 human liver cancer cells, with a halfmaximal
inhibitory concentration of 9.8±0.5μM, but with low toxicity in HL-7702 normal liver cells. Pt(II)-
Bpy induced DNA damage, which was demonstrated through a marked increase in the expression of cleavedpoly
(ADP ribose) polymerase (PARP) and gamma-H2A histone family member X and a decrease in PARP
expression. The interaction of Pt(II)-Bpy with DNA at the molecular level was most likely through an intercalation
mechanism, which might be evidence of DNA damage. Pt(II)-Bpy initiated cell cycle arrest at the S phase
in HepG2 cells. It also caused severe loss of the mitochondrial membrane potential; a decrease in the expression
of caspase-9 and caspase-3; an increase in reactive oxygen species levels; the release of cytochrome c and apoptotic
protease activation factor; and the activation of caspase-9 and caspase-3 in HepG2 cells, which in turn
resulted in apoptosis. Meanwhile, changes in p53 and related proteins were observed including the upregulation
of p53, the phosphorylation of p53, p21, B-cell lymphoma-2-associated X protein, and NOXA; and the downregulation
of B-cell lymphoma 2. Moreover, Pt(II)-Bpy displayed marked inhibitory effects on tumor growth in
the HepG2 nude mouse model.

Conclusion:
Pt(II)-Bpy is a potential candidate for cancer chemotherapy.
In Silico Target Identification and Molecular Docking Studies of Natural Cytotoxic Compound Borivilianoside H
Authors: Gupta S. Dutta , Kohli P. 
Published: November 2019

Background:Borivilianoside H is a naturally occurring anti-cancer compound with known cytotoxicity against human colorectal cancer cell line (HCT-116) and human adenocarcinoma cell line (HT-29). The present study describes the pharmacophore modelling, molecular docking, and molecular dynamics simulation approaches to predict the target proteins of borivilianoside H along with its binding affinity to the selected proteins.Methods:A 3-dimensional structure of borivilianoside H was constructed using Avogadro from its 2-D coordinates retrieved from the Pubchem Compound database. Target proteins associated with cancers were identified based on the 95% normalized fit score of PharmMapper. The crystal structures of the targets were retrieved from Protein Data Bank and molecular docking was performed with Autodock Vina 1.1.2. MD simulations were carried out via Google Cloud Platform. ADMET characteristics for borivilianoside H were determined using admetSAR web server.Results:Among the selected 7 top-ranked target proteins, fibroblast activation protein (FAP) exhibited the highest binding affinity followed by serum albumin (ALB), bone morphogenetic protein 2 (BMP2) and kinesin-like protein 11 (KIF11). However, the best fit was found with KIF11, where both the steroidal and oligosaccharide moieties of borivilianoside H were involved in interacting with the protein cavity. KIF11 was thus found to be the most suitable target for the anti-cancer effect of borivilianoside. ADMET analysis revealed its suitability as an intravenous drug.Conclusions:The targets predicted using this approach will serve as leads for the possible use of borivilianoside H, one of the active ingredients of Chlorophytum borivilianum as an anti-cancer drug.
INTERSECTING OPPRESSION OF GENDER AND RACE IN TONI MORRISON’S THE BLUEST EYE AND GOD HELP THE CHILD
Authors: Nurhayati Ari 
Published: November 2019

White domination in America can make white ideology of beauty spread and influence the African-American society. Toni Morrison’s novels, The Bluest Eye and God Help the Child, depict the influence. This study attemps to uncover the intersecting oppression of race and gender in the novels and to explain how African-American women cope with the oppression. This study is descriptive qualitative research. The data sources are Morrison’s novels The Bluest Eyeand God Help the Child. The study has two findings. Firstly, African-American society experiences oppression as an impact of the white beauty hegemony. The most disadvantageous oppression is the internalization of white beauty values. Holding such values makes African-American women feel inferior and hate their own physical characteristics that are far from the white ideal of beauty. Meanwhile, African-American women who have darker skin colors experience the hardest oppression because they also become the victims of oppression committed by some circles of African-American society, which tend to consider them unequal. It reflects the complexity of oppression experienced by African-American women. Secondly,self-consciousness is the main factor of attempts to release them from the oppression. Without self-consciousness, African-American women can be trapped in values that deteriorate their self-pride of identity.Keywords: intersecting oppression, African-American women, skin color
Health Researchers’ Use of Social Media: Scoping Review
Authors: Dol Justine , Tutelman Perri R , Chambers Christine T , Barwick Melanie , Drake Emily K , Parker Jennifer A , Parker Robin , Benchimol Eric I , George Ronald B , Witteman Holly O 
Published: November 2019

Background
Health researchers are increasingly using social media in a professional capacity, and the applications of social media for health researchers are vast. However, there is currently no published evidence synthesis of the ways in which health researchers use social media professionally, and uncertainty remains as to how best to harness its potential.

Objective
This scoping review aimed to explore how social media is used by health researchers professionally, as reported in the literature.

Methods
The scoping review methodology guided by Arksey and O’Malley and Levac et al was used. Comprehensive searches based on the concepts of health research and social media were conducted in MEDLINE, EMBASE, CINAHL, PsycINFO, ERIC, and Web of Science databases, with no limitations applied. Articles were screened at the title and abstract level and at full text by two reviewers. One reviewer extracted data that were analyzed descriptively to map the available evidence.

Results
A total of 8359 articles were screened at the title and abstract level, of which 719 were also assessed at full text for eligibility. The 414 articles identified for inclusion were published in 278 different journals. Studies originated from 31 different countries, with the most prevalent being the United States (52.7% [218/414]). The health discipline of the first authors varied, with medicine (33.3% [138/414]) being the most common. A third of the articles covered health generally, with 61 health-specific topics. Papers used a range of social media platforms (mean 1.33 [SD 0.7]). A quarter of the articles screened reported on social media use for participant recruitment (25.1% [104/414]), followed by practical ways to use social media (15.5% [64/414]), and use of social media for content analysis research (13.3% [55/414]). Articles were categorized as celebratory (ie, opportunities for engagement, 72.2% [299/414]), contingent (ie, opportunities and possible limitations, 22.7% [94/414]) and concerned (ie, potentially harmful, 5.1% [21/414]).

Conclusions
Health researchers are increasingly publishing on their use of social media for a range of professional purposes. Although most of the sentiment around the use of social media in health research was celebratory, the uses of social media varied widely. Future research is needed to support health researchers to optimize their social media use.
A Mobile Health App to Improve HIV Medication Adherence: Protocol for a Pilot Randomized Controlled Trial
Authors: Ramsey Susan , Ames Evan , Uber Julia , Habib Samia , Clark Seth 
Published: November 2019

Background
Adherence to antiretroviral therapy (ART) is essential for allowing persons living with HIV to live longer, healthier lives. However, a large portion of this population has suboptimal adherence and are not virally suppressed. Conventional interventions aimed at improving ART adherence lack portability and scalability, and improvements in adherence are not often sustained. Mobile health (mHealth) ART interventions offer a low-cost and accessible method of improving adherence, but many have limited functionality and do not offer comprehensive support. The combination of an mHealth intervention with a face-to-face adherence intervention and interactive health coaching feature may offer sufficient support in a manner that is sensitive to resource limitations that are often found in HIV treatment settings. This paper details the protocol of a study designed to evaluate the potential of an enhanced mHealth intervention for improving ART adherence.

Objective
The primary objective of this study is to assess the feasibility and acceptability of the Fitbit Plus app enhanced with a face-to-face LifeSteps session (Fitbit Plus condition) for improving ART adherence. In addition, we will determine the preliminary efficacy of the intervention by calculating treatment effect sizes.

Methods
This study will be conducted in 2 phases. The intervention will be developed and piloted with a small group of participants during phase 1. Pilot participants will provide feedback that will be used to refine the intervention for phase 2. In phase 2, a preliminary randomized controlled trial (RCT) comparing Fitbit Plus with a condition that approximates the standard of care (SOC) will be conducted with 60 persons living with HIV. Interviews will be conducted with RCT participants at baseline, and follow-up interviews will be conducted at 1, 3, 6, and 12 months. ART adherence is the primary outcome and will be monitored throughout the study via electronic pill boxes. Effect sizes will be generated using a fractional logit model estimated by generalized estimating equations.

Results
Phase 1 of this trial is complete; data collection for phase 2 is ongoing. Follow-ups with enrolled participants will conclude in January 2020.

Conclusions
This study will contribute to the literature on ART adherence and may produce an efficacious intervention. Owing to a small sample size, there may be insufficient power to detect statistically significant differences between Fitbit Plus and SOC. However, if Fitbit Plus is found to be acceptable and feasible and yields promising effect size estimates, this pilot study could serve as the foundation for a larger, fully powered trial of Fitbit Plus.

Trial Registration
ClinicalTrials.gov NCT02676128; https://clinicaltrials.gov/ct2/show/NCT02676128

International Registered Report Identifier (IRRID)
DERR1-10.2196/15356
Analisis penerimaan pemustaka terhadap electronic theses & dissertations di Perpustakaan Universitas Gadjah Mada
Authors: Kriswanto Y Rudi 
Published: November 2019

Introduction. Electronic theses & dissertations is one important service at Universitas Gadjah Mada (UGM) Library is to simplify the end-user reading service. However,it is still not understood whether the users are satisfied with the new service. An analysis is needed to examine the user acceptance of electronic theses & dissertations implemented by UGM Library.Data Collection Method. This paper used an acceptance analysis with a qualitative approach by interviewing three UGM students.Analysis Data. The paper applied Stuart Hall's theory of encoding-decoding.Results and Discussions. The results showed that the informants were categorises into two positions. One informant was in a dominant hegemonic position and the others were in negotiated positions. The background of the study program, the level of education and experiences, might influence the differences in the acceptance and interpretation of electronic theses & dissertations.Conclusions. There are various perspectives of how users examine to the electronic theses & dissertations at UGM Library.
Peran Perpustakaan Umum Kota Depok pada era teknologi digital
Authors: Rachman Margareta Aulia , Rachman Yeni Budi 
Published: November 2019

Introduction. The purpose of this study is to identify the roles of digital technology in the Depok City Public Library. The main challenge of the digital community is the increasing digital divide and the weakening of the identity of local communities which can result in low social participation and involvement in community issues. This study focuses on the characteristics of the people of Depok City to assess the role of public libraries that have the potential to be public spaces and places for social and physical meetings in the digital era.Data Collection Methods. This was a qualitative research with interviews involving librarians, library directors and users.Data Analysis. The data was analyzed by involving the process of data reduction, interpretation, and conclusion. The research setting at the chosen was the Depok City Public Library, close to the Capital of Indonesia.Results and Discussion. The results showed that Depok City public library of has several roles in the field of education, information, culture and recreation. These roles can be increased by involving technology as a means of library promotion.Conclusion and recommendations. The space and infrastructure in the library can be used optimally for various activities in order to enhance the role of the library as a public space and places for social and physical meetings in the digital era.