bluuebunny/crossref_metadata_2025_split huggingface dataset:
Checkpoint inhibitor-induced myocarditis and myasthenia gravis in a recurrent/metastatic thymic carcinoma patient: a case report
Authors: Szuchan Charles , Elson Leah , Alley Evan , Leung Kevin , Camargo Antonio Lewis , Elimimian Elizabeth , Nahleh Zeina , Sadler Diego 
Published: April 2020

Abstract

Background
Pembrolizumab, an immune checkpoint inhibitor (ICI), is an IgG4 antibody that blocks interaction between programmed cell death protein 1 and programmed death-ligand 1. Myocarditis, an immune-related adverse event, has been reported in thymic epithelial tumours. Pembrolizumab has also been associated with development/exacerbation of myasthenia gravis (MG).

Case summary
A 70-year-old woman with metastatic thymic cancer presented to the hospital with shortness of breath, 21 days after initiation of pembrolizumab. She was diagnosed with ICI-related myocarditis and was subsequently intubated due to respiratory failure. A dual-chamber pacemaker was placed due to complete heart block with asystole. Her troponin levels were elevated, an electrocardiogram was suspicious for myocardial infarction, but coronary angiogram revealed normal coronary arteries and endomyocardial biopsy confirmed the presence of myocarditis. Treatment was started with high-dose intravenous methylprednisolone and cardiovascular status improved. However, the patient was unable to be weaned from mechanical ventilation and tested positive for acetylcholine receptor binding/blocking antibodies due to de novo MG. After 50 days of hospitalization, she was discharged home in stable condition. A computed tomography scan was performed 6 weeks after pembrolizumab; results showed significant decrease/resolution of all measurable sites of metastatic disease in the lungs.

Discussion
This is the first reported case of a patient developing single-agent pembrolizumab-induced myocarditis concomitant with new-onset MG after treatment for advanced thymic malignancy. Additional studies are needed to explore the association between myocarditis, MG, and ICI therapy.
Might renin–angiotensin system blockers play a role in the COVID-19 pandemic?
Authors: Battistoni Allegra , Volpe Massimo 
Published: April 2020

AbstractSince December 2019, a new coronavirus, named SARS-CoV-2, has spread globally, affecting &gt;200 000 people worldwide with the so-called COVID-19 disease. The scientific community is actively and constantly working to identify the mechanisms involved in the diffusion of this virus and the pathogenesis of the infection, with its most frequent and severe complication, namely interstitial pneumonia. To date, SARS-CoV-2 is known to enter the host cells via the angiotensin-converting enzyme 2 protein. For this reason, the hypothesis that drugs capable of increasing the expression of this protein may have a role in the spread of the virus and in the symptomatology of affected patients has taken hold. The purpose of this Editorial is to briefly show the evidence currently available in this regard and to provide ideas for future research.
Distinct Patterns of Selective Sweep and Polygenic Adaptation in Evolve and Resequence Studies
Authors: Barghi Neda , Schlötterer Christian 
Published: April 2020

AbstractIn molecular population genetics, adaptation is typically thought to occur via selective sweeps, where targets of selection have independent effects on the phenotype and rise to fixation, whereas in quantitative genetics, many loci contribute to the phenotype and subtle frequency changes occur at many loci during polygenic adaptation. The sweep model makes specific predictions about frequency changes of beneficial alleles and many test statistics have been developed to detect such selection signatures. Despite polygenic adaptation is probably the prevalent mode of adaptation, because of the traditional focus on the phenotype, we are lacking a solid understanding of the similarities and differences of selection signatures under the two models. Recent theoretical and empirical studies have shown that both selective sweep and polygenic adaptation models could result in a sweep-like genomic signature; therefore, additional criteria are needed to distinguish the two models. With replicated populations and time series data, experimental evolution studies have the potential to identify the underlying model of adaptation. Using the framework of experimental evolution, we performed computer simulations to study the pattern of selected alleles for two models: 1) adaptation of a trait via independent beneficial mutations that are conditioned for fixation, that is, selective sweep model and 2) trait optimum model (polygenic adaptation), that is adaptation of a quantitative trait under stabilizing selection after a sudden shift in trait optimum. We identify several distinct patterns of selective sweep and trait optimum models in populations of different sizes. These features could provide the foundation for development of quantitative approaches to differentiate the two models.
Nudivirus Remnants in the Genomes of Arthropods
Authors: Cheng Ruo-Lin , Li Xiao-Feng , Zhang Chuan-Xi 
Published: April 2020

AbstractEndogenous viral elements (EVEs), derived from all major types of viruses, have been discovered in many eukaryotic genomes, representing “fossil records” of past viral infections. The endogenization of nudiviruses has been reported in several insects, leading to the question of whether genomic integration is a common phenomenon for these viruses. In this study, genomic assemblies of insects and other arthropods were analyzed to identify endogenous sequences related to Nudiviridae. A total of 359 nudivirus-like genes were identified in 43 species belonging to different groups; however, none of these genes were detected in the known hosts of nudiviruses. A large proportion of the putative EVEs identified in this study encode intact open reading frames or are transcribed as mRNAs, suggesting that they result from recent endogenization of nudiviruses. Phylogenetic analyses of the identified EVEs and inspections of their flanking regions indicated that integration of nudiviruses has occurred recurrently during the evolution of arthropods. This is the first report of a comprehensive screening for nudivirus-derived EVEs in arthropod genomes. The results of this study demonstrated that a large variety of arthropods, especially hemipteran and hymenopteran insects, have previously been or are still infected by nudiviruses. These findings have greatly extended the host range of Nudiviridae and provide new insights into viral diversity, evolution, and host–virus interactions.
Unprecedented Intraindividual Structural Heteroplasmy in Eleocharis (Cyperaceae, Poales) Plastomes
Authors: Lee Chaehee , Ruhlman Tracey A , Jansen Robert K 
Published: April 2020

AbstractPlastid genomes (plastomes) of land plants have a conserved quadripartite structure in a gene-dense unit genome consisting of a large inverted repeat that separates two single copy regions. Recently, alternative plastome structures were suggested in Geraniaceae and in some conifers and Medicago the coexistence of inversion isomers has been noted. In this study, plastome sequences of two Cyperaceae, Eleocharis dulcis (water chestnut) and Eleocharis cellulosa (gulf coast spikerush), were completed. Unlike the conserved plastomes in basal groups of Poales, these Eleocharis plastomes have remarkably divergent features, including large plastome sizes, high rates of sequence rearrangements, low GC content and gene density, gene duplications and losses, and increased repetitive DNA sequences. A novel finding among these features was the unprecedented level of heteroplasmy with the presence of multiple plastome structural types within a single individual. Illumina paired-end assemblies combined with PacBio single-molecule real-time sequencing, long-range polymerase chain reaction, and Sanger sequencing data identified at least four different plastome structural types in both Eleocharis species. PacBio long read data suggested that one of the four E. dulcis plastome types predominates.
Use of Informal Support as a Predictor of Home- and Community-Based Services Utilization
Authors: Robinson Kristen N , Menne Heather L , Gaeta Raphael 
Published: April 2020

Abstract

Objectives
Home- and community-based services (HCBS) help older adults remain living safely in their homes by delaying or preventing the need for institutionalization. This analysis is guided by the Andersen Behavioral Model of Health Services Use to examine the association between informal support and use of HCBS.

Method
Health and Retirement Study data from 2011 and 2012 are used in the bivariate analyses and multivariate logistic regression models to examine differences in HCBS utilization among extremely vulnerable older adults who have informal caregivers and those who do not.

Results
For extremely vulnerable older adults who report difficulties with any instrumental or basic activities of daily living, use of HCBS is not strongly associated with access to informal caregivers. However, for this same population of extremely vulnerable older adults, those who live alone have roughly 3 times the odds of using any HCBS compared to those who do not live alone.

Discussion
Among already vulnerable older adults, this study revealed that living alone is an important enabling factor of the Andersen Behavioral Model as applied in HCBS research. Further investigation is needed to see if more resources should be allocated to senior centers and local providers to identify vulnerable older adults who live alone and may have unmet needs.
Ground instability of sinkhole areas indicated by elastic moduli and seismic attributes
Authors: Wadas S H , Tschache S , Polom U , Krawczyk C M 
Published: April 2020

SUMMARY
Elastic moduli derived from vertical seismic profiles (VSPs) and 2-D SH-wave reflection seismic profiles are used to characterize mechanical properties of rocks in sinkhole areas. VP and VS were used to calculate the Poisson’s ratio and the dynamic shear modulus. The study shows that 2-D shear wave reflection seismics is suited to depict the heterogeneities of the subsurface induced by subsurface erosion. Low shear wave velocities of ca. 120–350 m s–1 and low shear strength values between 25 and 250 MPa are identified for the subsurface erosion horizon that consists of soluble Permian evapourites and the disturbed overlying deposits. These low values are a result of cavities and fractures induced by dissolution, creating unstable zones. In compliance with the shear modulus the Poisson’s ratio derived from the VSPs shows values of 0.38–0.48 for both the presumed subsurface erosion horizon, and the deposits above. This is a further indicator of reduced underground stability. In the VSPs, anomalies of the shear modulus and the Poisson’s ratio correlate with low electrical resistivities of less than 10 Ωm from borehole logs, indicating high conductivity due to fluid content. Further investigation reveals a conversion of S-to-P wave for the subsurface erosion horizon, which is probably the result of dipping layers and an oriented fracture network. Seismic attribute analysis of the 2-D sections shows strong attenuation of high frequencies and low similarity of adjacent traces, which correlate with the degree of subsurface erosion induced wave disturbance of the underground.
Magnetic anisotropy reveals Acadian transpressional fabrics in an Appalachian ophiolite (Thetford Mines, Canada)
Authors: Di Chiara Anita , Morris Antony , Anderson Mark W , Menegon Luca , Tremblay Alain 
Published: April 2020

SUMMARY
Magnetic anisotropy has proved effective in characterizing primary, spreading-related magmatic fabrics in Mesozoic (Tethyan) ophiolites, for example in documenting lower oceanic crustal flow. The potential for preservation of primary magnetic fabrics has not been tested, however, in older Palaeozoic ophiolites, where anisotropy may record regional strain during polyphase deformation. Here, we present anisotropy of magnetic susceptibility results from the Ordovician Thetford Mines ophiolite (Canada) that experienced two major phases of post-accretion deformation, during the Taconian and Acadian orogenic events. Magnetic fabrics consistent with modal layering in gabbros are observed at one locality, suggesting that primary fabrics may survive deformation locally in low strain zones. However, at remaining sites rocks with different magmatic origins have consistent magnetic fabrics, reflecting structurally controlled shape preferred orientations of iron-rich phases. Subhorizontal NW-SE-oriented minimum principal susceptibility axes correlate with poles to cleavage observed in overlying post-obduction, pre-Acadian sedimentary formations, indicating that the magnetic foliation in the ophiolite formed during regional NW-SE Acadian shortening. Maximum principal susceptibility axes plunging steeply to the NE are orthogonal to the orientation of regional Acadian fold axes, and are consistent with subvertical tectonic stretching. This magnetic lineation is parallel to the shape preferred orientation of secondary amphibole crystals and is interpreted to reflect grain growth during Acadian dextral transpression. This structural style has been widely reported along the Appalachian orogen, but the magnetic fabric data presented here provide the first evidence for transpression recorded in an Appalachian ophiolite.
Functional and recreational dog walking practices in the UK
Authors: Westgarth Carri , Christley Robert M , Marvin Garry , Perkins Elizabeth 
Published: April 2020

AbstractDog walking is a popular everyday activity known to contribute considerably to human health through motivating substantial additional physical activity. However, despite recognition that walking with a dog is substantially different from walking without a dog, little is known sociologically about the practices of dog walking. This study used in-depth interviews with 38 dog owners, combined with autoethnographical observation of dog walking. The aim was to investigate the types of dog walks that occur and the implications of this for the promotion of dog walking to increase human and animal wellbeing. Two distinct types of dog walking were found that had differing influencers and resulting experiences. Functional walks were purposed through feelings of guilt to provide the dog with a convenient form of exercise but were less pleasurable for the owner. In contrast, recreational walks provided significant owner stress-relief and were longer, typically during pleasant weather and at weekends, in less urban environments, and involved more members of the household. Limitations on time availability, conducive weather or accessibility of desirable physical environments for dog walking, generated functional rather than recreational dog walks. These findings have implications for interventions aiming to promote dog walking and for policy relating to the availability of safe and suitable green spaces for encouraging dog walking.
Prevention of premature death and seizures in a Depdc5 mouse epilepsy model through inhibition of mTORC1
Authors: Klofas Lindsay K , Short Brittany P , Zhou Chengwen , Carson Robert P 
Published: April 2020

Abstract
Mutations in DEP domain containing 5 (DEPDC5) are increasingly appreciated as one of the most common causes of inherited focal epilepsy. Epilepsies due to DEPDC5 mutations are often associated with brain malformations, tend to be drug-resistant, and have been linked to an increased risk of sudden unexplained death in epilepsy (SUDEP). Generation of epilepsy models to define mechanisms of epileptogenesis remains vital for future therapies. Here, we describe a novel mouse model of Depdc5 deficiency with a severe epilepsy phenotype, generated by conditional deletion of Depdc5 in dorsal telencephalic neuroprogenitor cells. In contrast to control and heterozygous mice, Depdc5-Emx1-Cre conditional knockout (CKO) mice demonstrated macrocephaly, spontaneous seizures and premature death. Consistent with increased mTORC1 activation, targeted neurons were enlarged and both neurons and astrocytes demonstrated increased S6 phosphorylation. Electrophysiologic characterization of miniature inhibitory post-synaptic currents in excitatory neurons was consistent with impaired post-synaptic response to GABAergic input, suggesting a potential mechanism for neuronal hyperexcitability. mTORC1 inhibition with rapamycin significantly improved survival of CKO animals and prevented observed seizures, including for up to 40 days following rapamycin withdrawal. These data not only support a primary role for mTORC1 hyperactivation in epilepsy following homozygous loss of Depdc5, but also suggest a developmental window for treatment which may have a durable benefit for some time even after withdrawal.
Experimental and numerical investigation of a novel photovoltaic/thermal system using micro-channel flat loop heat pipe (PV/T-MCFLHP)
Authors: Chen Fucheng , Hu Menglong , Badiei Ali , Yu Min , Huang Zicong , Wang Zhangyuan , Zhao Xudong 
Published: April 2020

Abstract
In this paper, a novel photovoltaic/thermal system using micro-channel flat loop heat pipe (PV/T-MCFLHP) is proposed, and the thermal and electrical performance of the system is investigated theoretically and experimentally. The variations of temperatures were analysed, and the efficiency of the system was calculated under different conditions, i.e. simulated solar radiation, water flow rate and refrigerant filling ratio. The maximum overall efficiency of the system was found to be 51.3%, the thermal efficiency 43.8% and the electrical efficiency 7.5% with the refrigerant filling ratio of 25%, simulated solar radiation of 800 W/m2 and water flow rate of 400 L/h. Test results were compared with simulation results, and the recorded average error was 10.2%.
What antimicrobial stewardship strategies do NHS commissioning organizations implement in primary care in England?
Authors: Allison Rosalie , Lecky Donna M , Beech Elizabeth , Costelloe Céire , Ashiru-Oredope Diane , Owens Rebecca , McNulty Cliodna A M 
Published: April 2020

Abstract

Objectives
To identify and explore strategies that English NHS commissioning organizations implemented to improve antimicrobial stewardship (AMS) within primary care.

Methods
Questionnaire sent to the medicines management teams (MMTs) of all 209 clinical commissioning groups (CCGs) in England, in 2017.

Results
A total of 89% (187/209) of all English CCGs responded to the questionnaire; 74% of responding CCGs (123/167) had a prescribing incentive/engagement scheme, with MMTs representing 88% (90/102) considering incentive schemes successful or very successful for prioritizing AMS in primary care, especially when linked to prescribing NHS Quality Premium indicators. AMS audits were considered successful or very successful by 91% (126/138) of responding CCGs, as they identify reasons for inappropriate prescribing and opportunities for future improvement. All responding MMTs (169/169 CCGs) reported feeding back local/national antimicrobial prescribing data to the general practices they commission, 85% (142/168) to their CCG/Commissioning Support Unit (CSU) board and only 33% (56/169) to out-of-hours services. Benchmarking prescribing data was reported as a powerful tool to engage practices, facilitating an element of competition and peer pressure.

Conclusions
National antimicrobial resistance improvement schemes, in particular the NHS England Quality Premium, have influenced CCG improvement priorities. Most CCGs now report successful improvement strategies including the use of both local and national antibiotic prescribing data to motivate improvements; these should be continued and extended to out-of-hours providers. As local audit data have helped to identify reasons for inappropriate prescribing and inform improvement planning, all organizations should adopt this strategy and include it in local quality improvement schemes, ensuring performance reporting to organizational board level.
Application of solid-phase DNA probe method with cleavage by deoxyribozyme for analysis of long non-coding RNAs
Authors: Arakawa Shizuka , Kamizaki Kohsuke , Kuwana Yusuke , Kataoka Naruki , Naoe Chieko , Takemoto Chie , Yokogawa Takashi , Hori Hiroyuki 
Published: April 2020

AbstractThe solid-phase DNA probe method is a well-established technique for tRNA purification. We have applied this method for purification and analysis of other non-coding RNAs. Three columns for purification of tRNAPhe, transfer-messenger RNA (tmRNA) and 16S rRNA from Thermus thermophilus were connected in tandem and purifications were performed. From each column, tRNAPhe, tmRNA and 16S rRNA could be purified in a single step. This is the first report of purification of native tmRNA from T. thermophilus and the purification demonstrates that the solid-phase DNA probe method is applicable to non-coding RNA, which is present in lower amounts than tRNA. Furthermore, if a long non-coding RNA is cleaved site-specifically and the fragment can be purified by the solid-phase DNA probe method, modified nucleosides in the long non-coding RNA can be analysed. Therefore, we designed a deoxyribozyme (DNAzyme) to perform site-specific cleavage of 16S rRNA, examined optimum conditions and purified the resulting RNA fragment. Sequencing of complimentary DNA and mass spectrometric analysis revealed that the purified RNA corresponded to the targeted fragment of 16S rRNA. Thus, the combination of DNAzyme cleavage and purification using solid-phase DNA probe methodology can be a useful technique for analysis of modified nucleosides in long non-coding RNAs.
Failure to Launch: Competing Institutional Logics, Intrapreneurship, and the Case of Chatbots
Authors: Belair-Gagnon Valerie , Lewis Seth C , Agur Colin 
Published: April 2020

AbstractThis article explores the institutional logics of intrapreneurial units, or groups within organizations that are designated to foster organizational innovation. Drawing on interviews with news intrapreneurs developing chatbots in news media organizations, this study shows that innovation can be stymied because of conflicting institutional logics. News intrapreneurs adopt a logic of experimentation, audience orientation, and efficiency-seeking, but that approach clashes with a journalistic logic prioritizing news workflows, formats, and associated autonomy for newsworkers. These clashing logics limit the adoption and influence of chatbots. This study illustrates the shaping influence of competing institutional logics and their negotiation in the development, deployment, and success or failure of intrapreneurial activities within organizations. The lesson is not that the existence of competing logics is, by default, a defeating proposition for innovation. Rather, this study advances scholarly understanding of the role of institutional logics in frustrating or facilitating technological adoption in organizations.