bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pendampingan Manajemen Pengelolaan Sampah di Masyarakat Desa Gagaksipat Kecamatan Ngemplak Kabupaten Boyolali
Authors: Winarto Winarto , Mahfiana Layyin , Rosyidah Zaidah Nur , Wicaksono Andi 
Published: February 2020

<p>This fostering program on waste management to the community of Gagaksipat is to provide the knowledge on how to manage waste properly. Further, it also aims not only to reduce waste but also to get beneficiat profit from the waste. The participants for this programm were 60 residents of Gagaksipat Village, Ngemplak, Boyolali through purposive sampling. As the partners for the programm is Team from the Kopen Tourism and Education Village (KWEK) Kopen 1/7 Ngadirejo Kartasura Sukoharjo. The technique carried out was in the form of training and continued with monitoring and evaluating at the end of the activity. The theoretical basis of this activity is UU No. 18 of 2008 concerning waste processing. The seriousness and necessity of waste management starts from upstream to downstream with the implementation of concepts such as 3R (Reuse, Reduse, Recycle) and then up to 5R (Revalue and Recovery). The results of this fostering progamm will make the community more aware of and contribute to good and healthy waste management; provide community supplies to manage organic waste properly through biopori activities and provide supplies to the community to manage inorganic waste through waste management skills.</p><p> </p><p>Program pendampingan ini bertujuan untuk memberikan pelatihan managemen pengelolaan sampah kepada masyarakat agar sampah dikelola dengan baik, tidak menumpuk yang mengakibatkan pencemaran lingkungan dan membahayakan kehidupan terutama bagi kesehatan masyarakat. Pendampingan ini mengupas tentang pengelolaan sampah dengan baik agar tidak membuat petaka, tetapi mendatangkan manfaat secara ekonomi bagi masyarakat.Subyek pendampingan ini adalah 60 warga Desa Gagaksipat Kecamatan Ngemplak Kabupaten Boyolali yang diperoleh melalui <em>purposive sampling</em>. Adapun mitra pendamping dalam pelatihan ini adalah Tim dari Kampung Wisata dan Edukasi Kopen (KWEK) Kopen 1/7 Ngadirejo Kartasura Sukoharjo.Teknik yang dilakukan dalam pendampingan ini berbentuk pelatihan dan dilanjutkan dengan monitoring dan evaluasi diakhir kegiatan. Landasan teori kegiatan ini adalah UU No. 18 Tahun 2008 tentang pengolahan sampah. Keseriusan dan keharusan pengelolaan sampah mulai diperhatikan dari hulu sampai hilir dengan implementasi konsep seperti 3R (<em>Reuse, Reduse, Recycle)</em> kemudian sampai 5R (<em>Revalue</em> dan <em>Recovery)</em>. Hasil dari pendampingan ini membuat masyarakat semakin menumbuhkan kesadaran dan peranan terhadap pengelolaan sampah yang baik dan sehat; memberikan bekal masyarakat untuk mengelola sampah organik dengan baik melalui kegiatan biopori dan memberikan bekal kepada masyarakat untuk mengelola sampah anorganik melalui keterampilan pengelolaan sampah.</p>
Optimization and scale-up of an LED-illuminated microalgal photobioreactor for wastewater treatment
Authors: Silva L. M. L. , Santiago A. F. , Silva G. A. , Castro A. L. P. , Bastos L. S. , Periard L. , Vassoler F. 
Published: February 2020

Abstract
The use of light-emitting diode (LED)-illuminated photobioreactors with microalgae has been extensively studied for wastewater treatment. Most studies have used isolated microalgae species; however, this practice does not match the reality of conditions in wastewater treatment plants. Operational conditions that promote greater growth of algal biomass and that remove pollutants most effectively are disputed in the literature. In this context, LED-illuminated photobioreactors with microalgae were evaluated using multivariate analysis in order to optimize removal of pollutants (nitrogen, phosphorus, and carbonaceous organic matter). Three variables were evaluated: operating time, LED wavelength, and luminous flux intensity. A microalgae consortium was used in the photobioreactor. In addition to the LED-illuminated photobioreactors, control photobioreactors illuminated by sunlight were also operated. Using the results obtained in the optimization, a scaled-up reactor approximately 8.5 times larger in volume was operated to evaluate if the behavior would be maintained. The best operational conditions for the removal of pollutants were observed in LED-illuminated photobioreactors operated under a light intensity of 700 μmol·m−2s−1 for 15 days. Under these conditions, it was possible to remove 89.97% of carbonaceous organic matter, 86.50% of nitrogen, and 30.64% of phosphorus. The scaled-up photobioreactor operated with similar performance.
SOX4 Serves an Oncogenic Role in the Tumourigenesis of Human Breast Adenocarcinoma by Promoting Cell Proliferation, Migration and Inhibiting Apoptosis
Authors: Zhang Junhe , Chai Shujie , Ruan Xinyu 
Published: February 2020

Background:Breast cancer is among the most common malignant cancers worldwide, and breast adenocarcinoma in glandular tissue cells has excessive metastasis and invasion capability. However, little is known on the molecular process by which this disease develops and progresses.Objective:In this study, we explored the effects of sex-determining region Y-box 4 (SOX4) protein on proliferation, migration, apoptosis and tumourigenesis of breast adenocarcinoma and its possible mechanisms.Methods:The SOX4 overexpression or knockdown Michigan Cancer Foundation-7 (MCF-7) cell lines were established. Among the SOX4 overexpression or MCF-7 knockdown cell lines, proliferation, migration ability and apoptosis rate were detected. The expression levels of apoptosis-related proteins (Bax and Cleaved caspase-3) were analysed using Western blot. The effect of SOX4 on tumourigenesis was analysed using the clone formation assay in vitro and tumour xenograft experiment in nude mice.Results:Compared with the overexpression of control cells, proliferation and migration ability of SOX4 overexpression cells significantly increased, the apoptosis rate significantly decreased in addition to the expression levels of Bax and Cleaved caspase-3 (P < 0.05). Compared with the knockdown of control cells, proliferation and migration ability of SOX4 knockdown cells significantly decreased, and the apoptosis rate and expression levels of Bax and Cleaved caspase-3 significantly increased (P < 0.05). Clone formation and tumour growth abilities of SOX4 overexpression cells were significantly higher than those of the control cells (P < 0.05), whereas SOX4 knockdown cells had the opposite effect.Conclusion:SOX4 plays an oncogenic role in breast adenocarcinoma tumourigenesis by promoting cell proliferation, migration and inhibiting apoptosis. It can be used as a potential molecular target for breast cancer gene therapy.
Microwave-assisted Cobalt-copper Dual Catalyzed Ligand Free C-Se Cross-coupling
Authors: Kundu Debasish , Roy Anup , Panja Subir , Singh Raj K. 
Published: February 2020

Background:
Organoselenides are important building blocks of several biologically important
molecules and natural products. Several protocols have been developed by chemists for their synthesis.
Transition metal-catalyzed cross-coupling is a powerful tool for this purpose in the last two decades. Various
transition metal catalysts e.g. Pd, Ni, Cu, In etc. have been used for performing C-Se cross-coupling
in the presence or absence of ligands.

Objective:
Development of a sustainable protocol for transition metal-catalyzed C-Se cross-coupling is
the main objective of this research. Recently, Cobalt has been applied as a cheap and sustainable transition
metal catalyst in several organic reactions. This protocol is focused on applying cobalt salt as a catalyst
for performing C-Se cross-coupling for the first time.

Methods:
Co(acac)2 has been successfully employed for performing Se-arylations in the presence of CuI,
which acts as a co-catalyst under microwave irradiation. NMP was used as solvent and KOH as a reductant
in this reaction.

Results:
Both iodo-and bromoarenes have been used to perform C-Se cross-coupling with diaryl
diselenide under this Co/Cu dual catalytic system. The reaction was successful with both electrondonating
and withdrawing groups in ortho-, meta-, and para-positions in the aromatic ring of Bromo and
iodoarenes.

Conclusion:
This is an effective protocol for the preparation of organoselenides, catalyzed by cobalt in
the presence of copper. The mechanism has been established by several experimental techniques.
Biomimicry: An Overview of Structures, Designs and Materials Inspired from Nature
Authors: Vasanthanathan Arunachalam , Siddharth Uthirakumar , Vignesh Manivannan , Pravin Radhakrishnan 
Published: February 2020

Background:
Nature has always played a vital role in the evolution of life forms.
The design of products in accordance with nature’s design, popularly known as biomimicry,
had played a vital role in pushing the technology and product effectiveness to the next level.
Humans have long sought to mimic not just the design, but also the methodology adopted by
certain animals. For example, the walking technique of vertebrates has been effectively
mimicked for a quadruped robot to make a system more efficient by consuming less power.
Thus indirectly, nature acts as a driving factor in pushing technological growth.

Methods:
The principle objective of this paper is to provide an overview of popular bio
mimicked products inspired by nature. This paper emphasizes a wide variety of products
developed in the field of materials inspired by nature.

Results:
Wall-climbing robots, Sonar, X-ray imaging, Sandwich and Honeycomb structures
are some of the popular products and designs inspired by nature. They have resulted in better
designs, better products with improved efficiency and thus have proven to be better alternatives.
Some products and designs such as Samara drone, Riblet surfaces, DSSCs, Biomimetic
Drills and Water turbines have plenty of scopes to replace conventional products and
designs.

Conclusion:
While plenty of products, structures and designs have successfully replaced
older alternatives, there is still a large scope for biomimicry where it could potentially replace
conventional products and designs to offer better efficiency.
Pemberantasan korupsi anggota DPRD di era otonomi daerah
Authors: Murdiono Mukhamad 
Published: February 2020

Since the implementation of decentralization, the drastic changes of politic and governmental system has been occurred in Indonesia. One of those changes is the growing significant of legislature role. In autonomy of district era, legislature was growing to be powerful marked by the function of its role, as aspiration channel and executive controller. The empowerment of legislature role in autonomy era brings some problems, not only connected with legislature themselves but also with executive. The problem connected with legislature can be cited such as bad behaviors (manipulation of graduation, money politics, corruption, etc.). Those bad behaviors have been caused the degradation of DPRD image and decadency moral. Therefore, it is necessary to rebuild politic morality and to change the image of legislative members. In order to change the image of DPRD the things can be done are reformation and radical change of DPRD by all element of society. Besides that, it is necessary to review the relationship between DPRD and people. The members of DPRD should compromise their views as well as their own needs with the people's aspirations and needs.
Association Between Web-Based Physician Ratings and Physician Disciplinary Convictions: Retrospective Observational Study
Authors: Liu Jessica Janine , Goldberg Hanna R , Lentz Eric JM , Matelski John Justin , Alam Asim , Bell Chaim M 
Published: February 2020

Background
Physician rating websites are commonly used by the public, yet the relationship between web-based physician ratings and health care quality is not well understood.

Objective
The objective of our study was to use physician disciplinary convictions as an extreme marker for poor physician quality and to investigate whether disciplined physicians have lower ratings than nondisciplined matched controls.

Methods
This was a retrospective national observational study of all disciplined physicians in Canada (751 physicians, 2000 to 2013). We searched ratings (2005-2015) from the country’s leading online physician rating website for this group, and for 751 matched controls according to gender, specialty, practice years, and location. We compared overall ratings (out of a score of 5) as well as mean ratings by the type of misconduct. We also compared ratings for each type of misconduct and punishment.

Results
There were 62.7% (471/751) of convicted and disciplined physicians (cases) with web-based ratings and 64.6% (485/751) of nondisciplined physicians (controls) with ratings. Of 312 matched case-control pairs, disciplined physicians were rated lower than controls overall (3.62 vs 4.00; P<.001). Disciplined physicians had lower ratings for all types of misconduct and punishment—except for physicians disciplined for sexual offenses (n=90 pairs; 3.83 vs 3.86; P=.81). Sexual misconduct was the only category in which mean ratings for physicians were higher than those for other disciplined physicians (3.63 vs 3.35; P=.003)

Conclusions
Physicians convicted for disciplinary misconduct generally had lower web-based ratings. Physicians convicted of sexual misconduct did not have lower ratings and were rated higher than other disciplined physicians. These findings may have future implications for the identification of physicians providing poor-quality care.
Віддалені результати корекції метаболічних порушень при синдромі полікістозних яєчників після лікування метформіном
Authors: Korytko O.O. , Pankiv I.V. 
Published: February 2020

Актуальність. Синдром полікістозних яєчників (СПКЯ) — поліморфне захворювання з надзвичайно варіабельною клінічною картиною та різними фенотипами, що відзначається у 5–10 % жінок репродуктивного віку. Протягом життя у жінок із СПКЯ в 10 разів підвищується ризик розвитку цукрового діабету (ЦД) 2-го типу, в 7 разів — артеріальної гіпертензії та ішемічної хвороби серця, в тому числі інфаркту міокарда, більш ніж удвічі — онкологічних захворювань яєчників, ендометрію, молочної залози. Мета дослідження — проведення аналізу динаміки показників інсулінорезистентності, дисліпідемії, порушеної толерантності до глюкози після лікування метформіном у пацієнток із СПКЯ з використанням показників індексів Caro та НОМА-IR. Матеріали та методи. Проаналізовані 26 історій хвороби пацієнток із СПКЯ, яким за період з 2012 по 2018 р. проводили терапію метформіном протягом шести місяців з моменту постановки діагнозу. Всім пацієнткам в 2018 р. було проведене дослідження ліпідного спектра крові, стандартний глюкозотолерантний тест і дослідження вмісту імунореактивного інсуліну в плазмі венозної крові. Оцінку стану вуглеводного обміну проводили за допомогою розрахункових показників індексів Caro та НОМА-IR. Результати. Результатом застосування метформіну у 26 жінок із СПКЯ й інсулінорезистентністю стало вірогідне зниження рівня імунореактивного інсуліну натще (з 21,4 ± 2,3 до 6,9 ± 0,8 мкОД/мл; р = 0,005) через 6 місяців, в той час як значущих змін рівня глікемії натще не спостерігалося. Відповідно, показник НОМА-IR знизився з 4,38 ± 1,26 до 1,17 ± 0,18 (р = 0,01), а індекс Caro підвищився з 0,24 ± 0,05 до 0,58 ± 0,14 (р = 0,02). Оцінка показників ліпідного обміну через 6 місяців після початку лікування метформіном підтвердила вірогідне зниження рівня холестерину з 5,4 ± 0,3 до 3,6 ± 0,5 ммоль/л (р = 0,01) і рівня тригліцеридів з 1,7 ± 0,2 до 0,8 ± 0,2 ммоль/л (р = 0,03). Обстеження, проведене у 2018 р. в цих же пацієнток, засвідчило збільшення маси тіла не тільки порівняно з показниками через 6 місяців після лікування метформіном, але і з початковими. Спостерігалося вірогідне підвищення рівня холестерину при обстеженні в 2018 р. до 6,7 ± 0,4 ммоль/л (р = 0,01 порівняно з показником після лікування метформіном). Висновки. Жінки із СПКЯ та інсулінорезистентністю, дисліпідемією мають найбільший ризик розвитку ЦД і серцево-судинної патології. Застосування метформіну розглядається як потенційно ефективний метод для подолання інсулінорезистентності, поліпшення гормональних, біохімічних параметрів і можливого зниження кардіоваскулярного ризику при СПКЯ.
PREPARATION DIFFERENTIAL CULTURE MEDIUM FOR CRYPTOCOCCUS NEOFORMANS FROM AQUEOUS EXTRACT OF LEAVES AND FLOWERS OF CHRYSANTHEMUM CINERARIAEFOLIUM
Authors: OBIED JASIM NEERAN , DAKEL MEHDI NOOR 
Published: February 2020

Objective: A new medium was prepared to isolate and diagnose the yeast Cryptococcus neoformans from flower and leaves aqueous extract of chrysanthemum.
Methods: Prepared differential culture medium for C. neoformans from aqueous extract of leaves and flowers of Chrysanthemum cinerariaefolium and chemical, spectral tests of the extracts were tested, in addition of gas chromatography (GC)–mass was used to diagnose phenolic compounds in both leaves and flowers.
Results: Showed that the yeast was grow with typical colonies on the new medium compared with other media which using in diagnosed of this yeast such as Staib agar and Sabourauds dextrose agar and unlike the yeast Candida albicans (as a negative control), which appeared in cream to white on this medium. Furthermore, the colonies are dark brown in color on flower chrysanthemum medium and light brown color on leaves chrysanthemum medium. In addition, the results of the chemical and spectral tests of the extracts confirmed that the plant contains many active compounds such as alkaloids, turbines, tannins, and phenols. The analysis of the extracts of phenolic compounds using GC–mass led to the diagnosis of five compounds in the leaf extract and nine compounds in the flower extract of this plant.
Conclusions: The media was prepared is differential medium that use to diagnosis of Cryptococcus such as Staib agar. Moreover, low economic cost, which consists of leaves and flowers of a plant available, abundance and the method of preparation is very simple.
VALIDATED STABILITY-INDICATING HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY METHOD FOR THE ESTIMATION OF TORSEMIDE
Authors: KV LALITHA , RAVEENDRA REDDY J  , DEVANNA N  
Published: February 2020

Objective: This assessment depicts the strength of exhibiting reverse-phase high performance liquid chromatography (RP-HPLC) method for the estimation of torsemide in pharmaceutical estimation structures.
Methods: In the present work, total protein-HPLC technique has been produced for the estimation of torsemide active pharmaceutical ingredient (API). Constrained degradation HPLC strategy was created with versatile mobile phase of methanol:water in the proportion of 90:10 v/v. The stream pace of 1 ml/min was utilized on Inertsil ODS 3V segment (250 mm×4.6 mm, 5 μm molecule size).
Results: The retention time of torsemide was seen at 8.267 min, method was validated for all validation parameters as per the International Council for Harmonization guidelines. The linearity range was 10–60 μg/ml, correlation coefficient was 0.9993, and percentage relative standard deviation in the precision studies was <2%, with percentage recovery 100.56–101.03 (within acceptable range of 98–102%). The assay result was found to be 100.88% (i.e., within 95–105%), passes the specifications for robustness parameters. Limit of detection of torsemide was found to be 0.0162 μg/ml and limit of quantitation of torsemide was found to be 0.0534 μg/ml.
Conclusion: The medication was exceptionally delicate to antacid pursued by at risk to corrosive, photolytic, warm, and oxidative conditions. The created and approved method showing HPLC technique is observed to be direct, exact, precise, explicit, and powerful. Henceforth, the technique can be utilized routinely for the estimation of torsemide API.
BIOCIDAL POTENTIALS OF STEM BARK EXTRACTS OF PSIDIUM GUAJAVA (LINN.) ON PANEL OF BACTERIAL STRAINS ASSOCIATED WITH DIARRHEA AND DYSENTERY
Authors: RASHEEDAT ABIDOYE OLADAYO , AYINDE AKINPELU DAVID , ALAYANDE KAZEEM ADEKUNLE , OLADELE AGUNBIADE MAYOWA 
Published: February 2020

Objective: Investigations were carried out on cidal effects of crude extract and fractions obtained from stem bark of Psidium guajava against a panel of 74 strains of Escherichia coli and Shigella dysenteriae implicated in diarrhea and dysentery infections.
Methods: Powdered sample of the stem bark was extracted in methanol/distilled water (3:2) and then partitioned into different organic solvents. The fractions obtained were subjected to antibacterial tests against a panel of bacterial strains. The minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) were determined using agar dilution method while the mode of action of the active fractions was investigated through time-kill dynamics and leakages of proteins and potassium ions from the cells.
Results: All partitioned fractions except the aqueous fraction exhibited antibacterial activities against the panel of bacterial strains at a final concentration of 10 mg/mL. The MIC exhibited by the crude extract against susceptible bacterial strains ranged between 1.56 mg/mL and 12.5 mg/mL, while the MIC for the four fractions ranged between 0.31 mg/mL and 5.0 mg/mL. The MBC ranged between 3.13–12.5 mg/mL and 0.63–5.0 mg/mL for the crude extract and fractions, respectively. The time-kill assay revealed that the percentage of the cells killed increase with an increase in the concentrations of the fractions as well as contact time intervals. Proteins and potassium ions leakages from the bacterial cells followed the same trend with that of time-kill assay.
Conclusion: The stem bark extracts of P. guajava exhibited appreciable bactericidal effects on bacterial strains associated with diarrhea and dysentery in humans.
IN VITRO ANTIMICROBIAL AND ANTI-INFLAMMATORY ACTIVITY OF METHANOL EXTRACT OF ERANTHEMUM CAPENSE
Authors: JOHN L ANOOPA , N KANNAPPAN , P MANOJKUMAR 
Published: February 2020

Objective: The present study was aimed to rationalize the scientific basis in traditional use of Eranthemum capense as an antibacterial, antifungal, and anti-inflammatory agent.
Methods: Agar well diffusion method is widely used to evaluate the antimicrobial activity of the E. capense aerial part of methanolic and ethyl acetate plant extracts. The same amount (15–20 mL) of Mueller-Hinton agar was poured on glass Petri plates of same size and allowed to solidify. E. capense aerial part of methanolic and ethyl acetate extracts was evaluated in vitro for their anti-inflammatory activities using the bovine serum albumin protein denaturation assay.
Results: The result of the study shows that methanolic exract (T3) of the plant, E. capense shows 16 mm zone of inhibition against Pseudomonas fluorescens, while the ethyl acetate extract of the same plant shows 14 mm zone of inhibition against P. fluorescens and E. coli. Hence the methanolic extract of T3 sample shows the antibacterial activity against gram negative bacteria, where as the ethyl acetate extract of T3 shows antibacterial activity against both gram positive and gram negative bacteria. The experimental report revealed that, the methanolic and ethyl acetate extract of the same plant produces zero percentage zone of inhibition against Aspergillus niger and Mucor, hence it does not show any antifungal activity.
Conclusion: It is observed that the EA and methanolic extract of E. capense can be used in the treatment of inflammation due to the significant percentage of inhibition of protein denaturation as well as its prove the good antimicrobial agent.