bluuebunny/crossref_metadata_2025_split huggingface dataset:
Using multimodal MRI to investigate alterations in brain structure and function in rats with type 2 diabetes.
Authors: Lawson Christopher M , Rentrup Kilian FG , Cai Xuezhu , Kulkarni Praveen P , Ferris Craig 
Published: March 2020

Abstract
Objectives This is an exploratory study using multimodal magnetic resonance imaging (MRI) to interrogate the brain of rats with type 2 diabetes (T2DM) as compared to controls. It was assumed there would be changes in brain structure and function that reflected the human disorder, thus providing a model system by which to follow disease progression with non-invasive MRI. Methods The transgenic BBZDR/Wor rat, an animal model of T2MD, and age-matched controls were studied for changes in brain structure using voxel-based morphometry, alteration in white and gray matter microarchitecture using diffusion weighted imaging with indices of anisotropy, and functional coupling using resting state BOLD functional connectivity. Images from each modality were registered to, and analyzed, using a 3D MRI rat atlas providing site-specific data on over 168 different brain areas. Results There was an overall reduction in brain volume focused primarily on somatosensory cortex, cerebellum and white matter tracts. The putative changes in white and gray matter microarchitecture were pervasive affecting much of the brain and not localized to any region. There was a general increase in connectivity in T2DM rats as compared to controls. The cerebellum presented with strong functional coupling to pons and brainstem in T2DM rats but negative connectivity to hippocampus. Conclusion Are the neuroradiological measures collected in BBBKZ/Wor rats using multimodal imaging methods common to the clinic, similar to those reported in T2DM patents? In comparison to the clinical findings, the data would suggest the BBBKZ/Wor rat is not an appropriate imaging model for T2DM.
Acacetin exert antioxidant potential against atherosclerosis through Nrf2 pathway in apoE-/- mice
Authors: Wu Yao , Song Fei , Li Yunda , Li Jingzhou , Cui Yukai , Hong Yixiang , Han Weimin , Wu Weiyin , Lakhani Ishan , Li Gang , Wang Yan 
Published: March 2020

Abstract
Background : Oxidative stress has a considerable influence on endothelial cell dysfunction and atherosclerosis. Acacetin, an anti-inflammatory and antiarrhythmic, is frequently used in the treatment of myocarditis, albeit its role in managing atherosclerosis is currently unclear. Thus, we evaluated the regulatory effects of acacetin in maintaining endothelial cell function and further investigated whether the flavonoid could attenuate atherosclerosis in apolipoprotein E deficiency (apoE -/- ) mice. Methods : Different concentrations of acacetin were tested on EA.hy926 cells, either induced or non-induced by human oxidized low density lipoprotein (oxLDL), to clarify its influence on cell viability, cellular reactive oxidative stress (ROS) level, apoptotic ratios and other regulatory effects. In vivo, apoE -/- mice were fed either a western diet or a chow diet. Acacetin prodrug (15mg/kg) was injected subcutaneously two times a day for 12weeks. The effects of acacetin on the atherosclerotic process, plasma inflammatory factors and lipid metabolism were also investigated. Results : Acacetin significantly increased EA.hy926 cell viability by reducing the ratios of apoptotic and necrotic cells at 3μM. Moreover, 3μM Acacetin clearly decreased ROS levels and enhanced reductase protein expression through MsrA and Nrf2 pathway through phosphorylation of Nrf2 and degradation of Keap1. In vivo , acacetin treatment remarkably attenuated atherosclerosis by increasing reductase levels in circulation and aortic roots, decreasing plasma inflammatory factor levels as well as accelerating lipid metabolism in Western diet-fed apoE -/- mice. Conclusions : Our findings demonstrate the anti-oxidative and anti-atherosclerotic effects of acacetin, in turn suggesting its potential therapeutic value in atherosclerotic-related cardiovascular diseases (CVD).
Flexor hallucis longus tendon transfer through a single incision in the treatment of chronic Achilles tendon rupture with large defect
Authors: Xu Yangbo , Li Cui , Xiang Feifan , Deng Yong , Lu Xiaobo , Wei Daiqing 
Published: March 2020

Abstract
Background To evaluate the medium-to-long term clinical outcome of flexor hallucis longus tendon transfer through a single incision in the treatment of chronic Achilles tendon rupture with a defect greater than 5 cm. Methods Clinical data of patients treated with flexor hallucis longus tendon transfer due to chronic Achilles tendon rupture between January 2009 and December 2016 were reviewed retrospectively. All cases were presented with Achilles tendon rupture for more than 4 weeks after injury. The defect of the Achilles tendon was greater than 5 cm after debridement in all patients. Clinical outcomes were assessed with AOFAS Ankle-Hindfoot Scale (AOFAS-AH), Achilles tendon Total Rupture Score (ATRS) and AOFAS Hallux Metatarsophalangeal-lnterphalangeal Scale (AOFAS-HM). Results 18 patients were followed successfully for 24-83 months (mean follow-up time of 43.2 months) and 5 were lost. According to the complete datasets obtained from 18 patients, none of the tendons re-ruptured during the follow-up. The AOFAS-AH and ATRS at last follow-up visit was 94.4 ± 3.2 (87-100) and 89.6 ± 5.9 (72-98) respectively, which revealed statistically significant improvement from the preoperative score of 58.2 ± 6.3 (49-70) and 53.3 ± 7.3(42-68). The AOFAS-HM at last follow-up visit was 90.1 ± 5.2 (77-97). Conclusion The flexor hallucis longus tendon transfer through a single incision is a safe and simple method for chronic Achilles tendon rupture with minimal morbidity and complications.
Water quality analysis of municipality public water supply in the case of Asella town at the source of Ashebeka River, a cross-sectional study design.
Authors: Eshete Amde Eshete 
Published: March 2020

Abstract
Background Drinking water quality is the main concern because in developing countries like Ethiopia, where contaminated water was one of the main vehicles for the transmission of water borne diseases. Therefore, the objective of this research was to assess the physicochemical & bacteriological water quality in Asella town, Ethiopia. Methods A cross-sectional study was carried out from November, 2015 to April, 2016 G.C. 8 liter composite water samples in plastic bottles packed cold chain had been taken by the investigator from eight different sites with two round totally 192 parameters were analyzed. A standard instrument like AAS for heavy metal analysis was using APAH procedures. Statistical analysis was done by the use of software for data entry Epi info 7 & Minitab 19.2. Results The first round of laboratory had showed that mean± SD TH 59.5± 7.4 mg/L, COD 30± 25.8mg/L, calcium 15± 4.3 mg/L, magnesium 5.3± 0.9 mg/L, BOD5 3± 2.6 mg/L and Nitrate 1.5± 0.1mg/L. The result of microbial analysis was 180CFU/100ml for TF & FC, 40CFU/100ml was E.coli detected in the first study site. The second round physical analysis had showed that municipality water was colourles, odourles & tasteless. But the Mean ± SD turbidity was 32.6± 63.4 mg/L. The mean± SD pH 4.5±0.8,EC 101.8±23.1,TH 24± 2.5 mg/L, calcium 6.9± 0.8 mg/L, magnesium 1. ± 0.6 mg/L, Fluoride 0.4± 0.2mg/L, aluminum 0.3± 0.2 mg/L, DO 4.6± 1.2 mg/L, nitrate 3.3± 2.5 mg/L, nitrite 0.9± 2/5 mg/L, zinc 0.3± 0.4 mg/L, CO32- 7. ± 6 mg/l, chloride 6± 1.3 mg/L, alkalinity 6± 4.9 mg/L, total solid 96± 50.3 mg/L, TDS 62± 14.8 mg/L, sodium 3.9± 2.4 mg/L, potassium 1.7± 0.1 mg/L, iron 0. ± 1mg/L and ammonia 0.9± 0.3 mg/L. There was strong pair wise statistical correlation among water quality parameters with p value < 0.05 Conclusions The water quality analysis was below the limit of WHO standard, but some chemical, turbidity & microbes need a critical attention. So, efficient water treatment must be monitored by the municipality & homemade water treatment by urban community
Effect of intravitreal antibiotic injections on culture-negative keratitis-induced endophthalmitis
Authors: Sim Ha Eun , Kang Min Ji , Park Jae Yong , Kim Jae Suk , Hwang Je Hyung 
Published: March 2020

Abstract
Background: This study aimed to evaluate treatment outcomes of culture-negative keratitis-induced endophthalmitis (CNKE) . Methods: We reviewed the medical records of 13 patients with CNKE, who underwent treated from January 2011 to December 2017. All patients were referred from the emergency room or outpatient department of local clinics. Outcome evaluation included the assessment of visual acuity and any requirements of evisceration/enucleation. Results: Thirteen eyes of 13 patients were included in this study. The mean age of the patients was 75.0 ± 13.3 years. All patients used antibiotic eye drops before the initial visit. Visual acuity values at the initial visit ranged from no light perception to 2/100, while final visual acuity values ranged from no light perception to 2/100. All patients underwent a vitreous tap and intravitreal ceftazidime/vancomycin injection. Two eyes underwent evisceration after intravitreal injection. Conclusions: Intravitreal antibiotic injections demonstrated favorable results for the treatment of patients with CNKE .
Improving geographical accessibility modeling for operational use by local health actors
Authors: Ihantamalala Felana Angella , Herbreteau Vincent , Révillion Christophe , Randriamihaja Mauricianot , Commins Jérémy , Andréambeloson Tanjona , Rafenoarivamalala Feno H , Randrianambinina Andriamihaja , Cordier Laura F , Bonds Matthew H , Garchitorena Andres 
Published: March 2020

Abstract
Background Geographical accessibility to health facilities remains one of the main barriers to access care in rural areas of the developing world. Although methods and tools exist to model geographic accessibility, the lack of basic geographic information prevents their widespread use at the local level for targeted program implementation. The aim of this study was to develop very precise, context-specific estimates of geographic accessibility to care in a rural district of Madagascar to help with the design and implementation of interventions that improve access for remote populations. Methods We used a participatory approach to map all the paths, residential areas, buildings and rice fields on OpenStreetMap (OSM). We estimated shortest route from every household in the District to the nearest primary health care center (PHC) and community health site (CHS) with the Open Source Routing Machine (OSMR) tool. Then, we used remote sensing methods to obtain a high resolution land cover map, a digital elevation model and rainfall data to model travel speed. Travel speed models were calibrated with field data obtained by GPS tracking in a sample of 168 walking routes. Model results were used to predict travel time to seek care at PHCs and CHSs for all the shortest route estimated earlier. Finally, we integrated geographical accessibility results into an e-health platform developed with R Shiny. Results We mapped over 100,000 buildings, 23,000 km of footpaths, and 4,925 residential areas throughout Ifanadiana district; this data is freely available on OSM. We found that over three quarters of the population lived more than one hour away from a PHC, and 10-15% lived more than one hour away from a CHS. Moreover, we identified areas in the North and East of the district where the nearest PHC was further than 5 hours away, and vulnerable populations across the district with poor geographical access (>1 hour) to both PHCs and CHSs. Conclusion Our study demonstrates how to improve geographical accessibility modeling so that results can be context-specific and operationally actionable by local health actors. The importance of such approaches is paramount for achieving universal health coverage in rural areas throughout world.
Diversity and distribution of marine heterotrophic bacteria from a large culture collection
Authors: Sanz-Sáez Isabel , Salazar Guillem , Lara Elena , Royo-Llonch Marta , Sà Elisabet L. , Vaqué Dolors , Duarte Carlos M. , Gasol Josep M. , Pedrós-Alió Carlos , Sánchez Olga , Acinas Silvia G. 
Published: March 2020

Abstract
BackgroundIsolation of marine microorganisms is fundamental to gather information about their physiology, ecology and genomic content. To date, most of the bacterial isolation efforts have focused on the photic ocean leaving the deep ocean less explored. We have created a marine culture collection of heterotrophic bacteria (MARINHET) using a standard marine medium comprising a total of 1561 bacterial strains, and covering a variety of oceanographic regions from different seasons and years, from 2009 to 2015. Specifically, our marine collection includes isolates from both photic (817) and aphotic layers (744), including the oxygen minimum zone (OMZ, 362) and the bathypelagic (382), from the North Western Mediterranean Sea, the North and South Atlantic Ocean, the Indian, the Pacific, and the Arctic Oceans. With this study, we describe the taxonomy, the phylogenetic diversity and the biogeography of a fraction of the marine culturable microorganisms to enhance our knowledge about which heterotrophic marine isolates are recurrently retrieved across oceans and along all the water column. ResultsThe partial sequencing of the 16S rRNA gene of all isolates revealed that they mainly affiliate with the classes Alphaproteobacteria (35.9%), Gammaproteobacteria (38.6%), and phylum Bacteroidetes (16.5%). In addition, Alteromonas and Erythrobacter genera were found the most common heterotrophic bacteria in the ocean growing in solid agar medium. When comparing photic, OMZ, and bathypelagic isolates sequences, a 37% of them were 100% identical. This percentage increased up to 59% when the comparison was restricted between photic and aphotic sequences (OMZ and bathypelagic together) indicating the ubiquity of some bacterial isolates along the water column. Finally, we isolated three strains related with the genus Mesonia sp. that may represent new species since their whole 16S rRNA gene shared less than 95% sequence similarity with any other culture representative. ConclusionsOverall, this study highlights the relevance of culture dependent studies, with focus on marine isolated bacteria from different oceanographic regions and depths, to provide a more comprehensive view of marine microbial diversity.
Label-free quantitative proteomic analysis of the inhibition effect of Lactobacillus rhamnosus GG on Escherich coli biofilm formation in coculture
Authors: Song Huiyi , Lou Ni , Liu Jianjun , Xiang Hong , Shang Dong 
Published: March 2020

Abstract
Background: Escherich coli (E.coli) is the principal pathogen that causes biofilm formation; the latter is associated with infectious diseases and antibiotic resistance. In our previous work, we demonstrated that probiotic microcapsules have superior biofilm inhibition capacity compared to probiotic sterile culture supernatant. Herein, the mechanism of the inhibition effects was investigated using label-free quantitative proteomics analysis. Results: The proteomic analysis characterized a total of 1655 proteins in E.coli K12MG1655 and 1431 proteins in Lactobacillus rhamnosus GG (LGG). Among them, after coculture treatment, there were 262 and differentially expressed proteins that were specific for E.coli and 291 for LGG. The differentially expressed proteins after coculture were related to cellular metabolism, the stress response, transcription, and the cell membrane. In addition, we identified five strain-specific genes in E.coli and LGG, respectively, which were consistent with the proteomics results. Conclusions: These findings indicate that LGG microcapsules may inhibit E.coli biofilm inhibition by disrupting metabolic processes, particularly in relation to energy metabolism and stimulus responses, both of which are critical for the growth of LGG. Together, these findings increase our understanding of the interactions between bacteria under coculture conditions.
Treatment Effects of Reverse Total Shoulder Arthroplasty for Unilateral Cuff Tear Arthropathy – Outcomes at 6, 12, 24 and 60 Months and Confounders
Authors: Huber Jorg , Irlenbusch Ulrich , Kääb Max J , Reuther Falk , Kohut Georges , Judge Andy 
Published: March 2020

Abstract
Background Although shoulder arthroplasty is less common than knee or hip arthroplasty, the number of procedures being performed is increasing rapidly. The treatment effect is a simple method to measure outcome of joint replacement for each patient individually. The method was applied to measure outcome of total hip/knee arthroplasty but not yet for shoulder arthroplasty. Methods The patients with reversed total shoulder arthroplasty (RSA) of five European clinics were included in this prospective study. The treatment effects (TE) were calculated for each patient as complaint reduction/baseline complaints. 1 is the maximal treatment effect and corresponds to a patient without complaints. A positive number means amelioration, 0 = unchanged, a negative number means worse. The primary aim was to calculate the TE’s for RSA at 6, 12, 24, and 60 months postoperatively. The secondary aim was to analyze the influence of confounders (preoperative grade of cuff tear arthropathy, age, gender, dominance, side of the affected shoulder, general co-morbidities measured using ASA grade). Results 203 patients were included for this analysis of whom 183 patients had a complete 2 year follow up. Over the 24-months, the mean ASES score augmented significant from 20.5 to 78.7 (p<0.001). The 2 year treatment effects ranged from 1 to 0.09. We had no patient with a negative TE. A higher Hamada grade was associated with better TE’s (Hamada grade 4+ vs. 2, p-value 0.042). For age and dominant side there were weak associations where those aged 80+ and dominant side had better TE’s. The patients with higher ASA grade had lower TE’s (ASA grade 4+ vs. 1, p-value 0.013). The mean TE’s were 0.77 at 6-months, 0.81 12-months, 0.76 24-months and 0.73 at 60-months. Conclusions The treatment effects for reverse shoulder arthroplasty vary from 1 to 0.09. The treatment effects change little in the first five postoperative years (from 0.73 to 0.81). The confounders for better TE’s were: higher severity of cuff arthropathy (Hamada grade 3, 4 and 5), less co-morbidities (ASA Grade 1), higher age (80+) and dominant side. Gender did not influence the 2-year TE’s.
Th22 Cells Promote in vitro Proliferation and Cytokine Secretion in HaCaT and HRMC cells via Production of IL-22 and Stimulation of PI3K/AKT Signaling
Authors: Zhao Ling , Sun Dejun , Liu Tao , Jiang Zhenyu , Ye Zhuang 
Published: March 2020

Abstract
Background: T helper (Th) 22 cells function in the pathogenesis of systemic lupus erythematosus (SLE), but their exact role remains unclear. We examined in vitro interactions of Th22 cells with different target cells.Methods: Flow cytometry was used to isolate Th22 cells from the peripheral blood mononuclear cells (PBMCs) of 5 healthy subjects. The optimal co-culture ratio of effector:target (E:T) cells and the optimal co-culture concentration of recombinant interleukin (rIL) -22 were determined. The levels of IL-22, proliferation of target cells, and the levels proinflammatory cytokines in different culture systems with human keratinocyte cells (HaCaT) and human renal mesangial cells (HRMC) were determined. Western blotting was used to assess changes in the levels of proteins in the PI3K/AKT pathway.Results: The levels of IL-22 were greater when HaCaT and HRMC cells were co-cultured with Th22 cells or rIL-22, and co-culturing with contact had a greater effect than co-culturing without contact. The levels of cytokines were also significantly greater when target cells were co-cultured with Th22 cells. IL-22mAb reversed the effect of IL-22. Th22 cells significantly activated PI3K/AKT signaling in HaCaT and HRMC cells; LY294002 (an inhibitor of this pathway) reversed this effect and also downregulated the levels of proinflammatory cytokines; IL-22mAb had a similar but weaker effect. Conclusions: Our results suggest that Th22 cells promote cell proliferation and secretion of pro-inflammatory cytokines of their target cells via IL-22-mediated stimulation of PI3K/AKT signaling.
Localization of pulp stem cells in chronic pulpitis
Authors: Wu Yan , Zhou Caixia , Tong Xueying , Li Shue , Liu Jiarong 
Published: March 2020

Abstract
Background: Our study aimed to observe the distribution of stem cells in chronic pulpitis and to investigate the expression of specific molecules.
Methods: Extracted third molar teeth were collected and divided into two groups. Experiment group was from patients with previous history of spontaneous pain and diagnosed as having chronic pulpitis. Noncarious third molars were used as control group. Dental pulp tissues were collected from these two groups. Real-time RT-PCR test was used to detect the expression of several embryonic and dentinogenic genes in dental pulp. The expression of the mesenchymal cell markers (STRO-1, CD146) and stromal cell-derived factor 1α (SDF-1α)/CXC chemokine receptor 4 (CXCR4) proteins were examined by immunohistochemical and triple immunofluorescence analysis.
Results: ThemRNA levels of most embryonic and dentinogenic genes were not statistically different (p > 0.05) between the two groups. Immunohistochemical analysis revealed that in inflamed pulp, STRO-1- and CD146-positive cells mainly reside in two specific niches, both adjacent to inflammatory sites: one in the pulp core, and another in odontoblast layer. In contrast, fewer STRO-1- and CD146- positive cells appeared in the vicinity of the root apex. SDF-1α- and CXCR4-positive cells increased in zone I and II, and decreased in zone III, showing a significant correlation with STRO-1. Triple immunofluorescence analysis indicated that most STRO-1-positive cells overlapped with SDF-1α- and CXCR4-positive cells around the perivascular areas near the inflammatory site.
Conclusions: This study gave a direct observation of stem cells distribution in chronic pulpitis for the first time and implies the important role of SDF-1α/CXCR4signaling in stem cells-based therapies for reparative dentinogenesis.