bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fabrication of Polythiourea-Copper Complex Composite Membrane and its Anti-fouling Property
Authors: Margarito M T , Beltran A B , Promentilla MA , Orbecido A , Basilia B , Damalerio R , Bigol U 
Published: May 2020

Abstract
A Composite Flat Sheet Membrane Containing Polythiourea-Copper (PTU-Cu) Complex was fabricated through a two-step phase separation involving complexation and/or crosslinking of the polymer by copper ions (Cu2+) on the first step and non-solvent induced phase separation on the second step. The surface topography of the membrane was analysed using Atomic Force Microscope (AFM) in non-contact mode. The incorporation of copper at the surface was confirmed through Scanning Electron Microscope-Energy Dispersive X-ray (SEM-EDX) mapping wherein other elements such as Sulfur (S), Carbon (C) and Oxygen (O) of the polymer were conducted. The fabricated membrane was rigid as shown by high value (about 2.15 GigaPascal) of measured Young’s modulus using the Pinpoint Nanomechanical Analysis Mode of AFM. In addition, the surface charge and hydrophobicity were measured using the Electrostatic Force Microscope (EFM) and water contact angle respectively. The antifouling characteristics of the membrane was evaluated through antimicrobial membrane surface contact test wherein E. coli was used as test microorganism. Other membrane properties such as pore size distribution and pure water flux were measured using a porometer and a filtration apparatus.
Functional evaluation of prevascularization in one-stage versus two-stage tissue engineering approach of human bio-artificial muscle
Authors: Gholobova D , Terrie L , Mackova K , Desender L , Carpentier G , Gerard M , Hympanova L , Deprest J , Thorrez L 
Published: May 2020

AbstractA common shortcoming of current tissue engineered constructs is the lack of a functional vasculature, limiting their size and functionality. Prevascularization is a possible strategy to introduce vascular networks in these constructs. It includes among others co-culturing target cells with endothelial (precursor) cells that are able to form endothelial networks through vasculogenesis. In this paper, we compared two different prevascularization approaches of bio-artificial skeletal muscle tissue (BAM) in vitroandin vivo. In a one-stage approach, human muscle cells were directly co-cultured with endothelial cells in 3D. In a two-stage approach, a one week old BAM containing differentiated myotubes was coated with a fibrin hydrogel containing endothelial cells. The obtained endothelial networks were longer and better interconnected with the two-stage approach. We evaluated whether prevascularization had a beneficial effect onin vivoperfusion of the BAM and improved myotube survival by implantation on the fascia of thelatissimus dorsimuscle of NOD/SCID mice for 5 or 14 d. Alsoin vivo, the two-stage approach displayed the highest vascular density. At day 14, anastomosis of implanted endothelial networks with the host vasculature was apparent. BAMs without endothelial networks contained longer and thicker myotubesin vitro, but their morphology degradedin vivo. In contrast, maintenance of myotube morphology was well supported in the two-stage prevascularized BAMs. To conclude, a two-stage prevascularization approach for muscle engineering improved the vascular density in the construct and supported myotube maintenancein vivo.
Reactive cavitation erosion as a technique for production of functionalized copper hydroxychloride nanomaterials
Authors: Wright Jeremy W , Mitchell Brian S 
Published: May 2020

Abstract
The production of functionalized clinoatacamite (Cu2(OH)3Cl) nanoparticles from Reactive Cavitation Erosion (RCE) of copper in 1 M aqueous guanidine hydrochloride (GHCl) solution was investigated for applications as potential quantum magnet materials. As-synthesized nanomaterial was characterized by Diffuse-Reflectance Infrared Fourier-Transform Spectroscopy (DRIFTS), XRD, and TEM. These analyses were compared to nanoparticles produced from RCE of Cu in KCl and RCE of Cu in GHCl in oxygen-depleted (Ar) and oxygen-rich (compressed air, CA) solutions to identify possible reaction pathways.
Anti-arbitration injunctions: walking the tightrope
Authors: Garnett Richard 
Published: May 2020

Abstract
Anti-arbitration injunctions are a controversial issue in the field of international arbitration. While some commentators decry them as an interference with the autonomy and independence of arbitrators, English and other common law courts steadfastly refuse to renounce them entirely. By reference to the framework established in recent decisions of the English Court of Appeal, the article seeks to forge a middle path between making anti-arbitration injunctions available on a discretionary, case management basis and prohibiting them in all cases. An approach that respects both the rights of arbitrators to determine their own jurisdiction and the rights of courts to protect parties from abuse of the arbitral process is advocated.
Loss of TMEM106B leads to myelination deficits: implications for frontotemporal dementia treatment strategies
Authors: Zhou Xiaolai , Nicholson Alexandra M , Ren Yingxue , Brooks Mieu , Jiang Peizhou , Zuberi Aamir , Phuoc Hung Nguyen , Perkerson Ralph B , Matchett Billie , Parsons Tammee M , Finch NiCole A , Lin Wenlang , Qiao Wenhui , Castanedes-Casey Monica , Phillips Virginia , Librero Ariston L , Asmann Yan , Bu Guojun , Murray Melissa E , Lutz Cathleen , Dickson Dennis W , Rademakers Rosa 
Published: May 2020

Abstract
Genetic variants that define two distinct haplotypes at the TMEM106B locus have been implicated in multiple neurodegenerative diseases and in healthy brain ageing. In frontotemporal dementia (FTD), the high expressing TMEM106B risk haplotype was shown to increase susceptibility for FTD with TDP-43 inclusions (FTD-TDP) and to modify disease penetrance in progranulin mutation carriers (FTD-GRN). To elucidate the biological function of TMEM106B and determine whether lowering TMEM106B may be a viable therapeutic strategy, we performed brain transcriptomic analyses in 8-month-old animals from our recently developed Tmem106b−/− mouse model. We included 10 Tmem106b+/+ (wild-type), 10 Tmem106b+/− and 10 Tmem106−/− mice. The most differentially expressed genes (153 downregulated and 60 upregulated) were identified between Tmem106b−/− and wild-type animals, with an enrichment for genes implicated in myelination-related cellular processes including axon ensheathment and oligodendrocyte differentiation. Co-expression analysis also revealed that the most downregulated group of correlated genes was enriched for myelination-related processes. We further detected a significant loss of OLIG2-positive cells in the corpus callosum of Tmem106b−/− mice, which was present already in young animals (21 days) and persisted until old age (23 months), without worsening. Quantitative polymerase chain reaction revealed a reduction of differentiated but not undifferentiated oligodendrocytes cellular markers. While no obvious changes in myelin were observed at the ultrastructure levels in unchallenged animals, treatment with cuprizone revealed that Tmem106b−/− mice are more susceptible to cuprizone-induced demyelination and have a reduced capacity to remyelinate, a finding which we were able to replicate in a newly generated Tmem106b CRISPR/cas9 knock-out mouse model. Finally, using a TMEM106B HeLa knock-out cell line and primary cultured oligodendrocytes, we determined that loss of TMEM106B leads to abnormalities in the distribution of lysosomes and PLP1. Together these findings reveal an important function for TMEM106B in myelination with possible consequences for therapeutic strategies aimed at lowering TMEM106B levels.
Focal Electrical Stimulation of Cortical Functional Networks
Authors: Hu Jia Ming , Qian Mei Zhen , Tanigawa Hisashi , Song Xue Mei , Roe Anna Wang 
Published: May 2020

Abstract
Traditional electrical stimulation of brain tissue typically affects relatively large volumes of tissue spanning multiple millimeters. This low spatial resolution stimulation results in nonspecific functional effects. In addition, a primary shortcoming of these designs was the failure to take advantage of inherent functional organization in the cerebral cortex. Here, we describe a new method to electrically stimulate the brain which achieves selective targeting of single feature-specific domains in visual cortex. We provide evidence that this paradigm achieves mesoscale, functional network-specificity, and intensity dependence in a way that mimics visual stimulation. Application of this approach to known feature domains (such as color, orientation, motion, and depth) in visual cortex may lead to important functional improvements in the specificity and sophistication of brain stimulation methods and has implications for visual cortical prosthetic design.
Optimization of Extraction Conditions of Continentalic and Kaurenoic Acids from Aralia continentalis by HPLC/UV and Their Validation
Authors: Lee Ju Sung , Quilantang Norman G , Hahm Dae-Hyun , Kang Ki Sung , Jacinto Sonia D , Choi Yu-Jin , Lee Sang Cheon , Lee Sanghyun 
Published: May 2020

Abstract
Aralia continentalis (AC) is a perennial herb that has long been used as a traditional medicine for many diseases. Continentalic acid (CA) and kaurenoic acid (KA) are major diterpenoids in AC, which are known to exert various pharmacological activities. This study focuses on the optimization of the extraction of CA and KA from dried AC roots by evaluating the influence of different extraction conditions on their yield. Five extraction variables were examined: sample weight, solvent concentration, extraction time, solid matrix and the number of repeated extractions. The analytical method used in this study was also validated in terms of linearity, limit of detection, limit of quantification, precision and accuracy. The CA and KA yields were measured by high-performance liquid chromatography analysis. The results show that CA and KA were the highest when unpulverized samples (3.75 g) were subjected to a single extraction for 5 h using 50% ethanol (300 mL) as the solvent. These conditions are proposed for the optimization of the extraction of CA and KA from AC.
Economic Considerations in Infectious Diseases Emergency Response Preparedness: It’s All About the Point of View
Authors: Branch-Elliman Westyn , Safdar Nasia , Nelson Richard 
Published: May 2020

Abstract
Outbreaks and emergence of novel pathogens present a challenge in economic evaluations of prevention strategies, due to unusually high levels of risk aversion and uncertainty. Here, we discuss cost-effectiveness investigations and interpretation of economic analyses in the context of outbreak planning and containment, and outline considerations for providers, administrators, patients, and policy makers for infection emergency preparedness response.
Pulsed Levosimendan in advanced heart failure due to congenital heart disease: a case series
Authors: Cranley James , Hardiman Antonia , Freeman Leisa J 
Published: May 2020

Abstract

Background
Levosimendan is a non-adrenergic calcium-sensitizing agent with positive inotropic and vasodilatory effects. Its use in acute decompensation of heart failure is established. Good evidence now exists for repetitive infusions of Levosimendan to improve symptoms and reduce hospitalization in advanced heart failure (AdHF) populations. Its use in heart failure resulting from congenital heart disease is not yet commonplace.

Case summary
We present three cases in which pulsed Levosimendan was used in the management of AdHF secondary to underlying congenital heart disease. There was symptomatic and biomarker evidence of improvement.

Discussion
Intermittent Levosimendan may represent a valuable therapy to reduce hospitalization and improve quality of life in adults with congenital heart conditions.
The Social Costs of Side Trading
Authors: Attar Andrea , Mariotti Thomas , Salanié François 
Published: May 2020

Abstract
We study resource allocation under private information when the planner cannot prevent bilateral side trading between consumers and firms. Adverse selection and side trading severely restrict feasible trades: each marginal quantity must be fairly priced given the consumer types who purchase it. The resulting social costs are twofold. First, second-best efficiency and robustness to side trading are in general irreconcilable requirements. Second, there actually exists only one budget-feasible allocation robust to side trading, which deprives the planner from any capacity to redistribute resources between different types of consumers. We discuss the relevance of our results for insurance and financial markets.
C. Wilfred Jenks and the Futures of International Organizations Law
Authors: Sinclair Guy Fiti 
Published: May 2020

Abstract
This article situates C. Wilfred Jenks as a central figure in the emergence and development of the law of international organizations. Deeply informed by his work as a legal advisor at the International Labour Organization (ILO), Jenks’ scholarly writings during and immediately after World War II established a basis for, and elaborated the details of many aspects of, classical international organizations law. Moreover, the article argues that Jenks’ oeuvre also articulated a number of insights and approaches that, in retrospect, may be read as suggesting a series of alternative futures for international organizations law. By examining Jenks’ foundational works on international organizations law, therefore, the article seeks to recover aspects of Jenks’ thinking that might have led – and might still lead – the field to explore different paths.
Substrate-dependent CO2 fixation in heterotrophic bacteria revealed by stable isotope labelling
Authors: Spona-Friedl Marina , Braun Alexander , Huber Claudia , Eisenreich Wolfgang , Griebler Christian , Kappler Andreas , Elsner Martin 
Published: May 2020

ABSTRACTVirtually all heterotrophs incorporate carbon dioxide by anaplerotic fixation. Little explored, however, is the interdependency of pathways and rates of CO2fixation on the concurrent usage of organic substrate(s). Potentially, this could reveal which substrates out of a pool of dissolved organic carbon are utilised by environmental microorganisms. To explore this possibility, Bacillus subtilis W23 was grown in a minimal medium with normalised amounts of either glucose, lactate or malate as only organic substrates, each together with 1 g/L NaH13CO3. Incorporation of H13CO3− was traced by elemental analysis-isotope ratio mass spectrometry of biomass and gas chromatography-mass spectrometry of protein-derived amino acids. Until the late logarithmic phase, 13C incorporation into the tricarboxylic acid cycle increased with time and occurred via [4–13C]oxaloacetate formed by carboxylation of pyruvate. The levels of 13C incorporation were highest for growth on glucose and lowest on malate. Incorporation of 13C into gluconeogenesis products was mainly detected in the lactate and malate experiment, whereas glucose down-regulated this path. A proof-of-principle study with a natural groundwater community confirmed the ability to determine incorporation from H13CO3− by natural communities leading to specific labelling patterns. This underlines the potential of the labelling approach to characterise carbon sources of heterotrophic microorganisms in their natural environments.
Experimental assembly reveals ecological drift as a major driver of root nodule bacterial diversity in a woody legume crop
Authors: Ramoneda Josep , Le Roux Johannes J , Frossard Emmanuel , Frey Beat , Gamper Hannes Andres 
Published: May 2020

ABSTRACT
Understanding how plant-associated microbial communities assemble and the role they play in plant performance are major goals in microbial ecology. For nitrogen-fixing rhizobia, community assembly is generally driven by host plant selection and soil conditions. Here, we aimed to determine the relative importance of neutral and deterministic processes in the assembly of bacterial communities of root nodules of a legume shrub adapted to extreme nutrient limitation, rooibos (Aspalathus linearis Burm. Dahlgren). We grew rooibos seedlings in soil from cultivated land and wild habitats, and mixtures of these soils, sampled from a wide geographic area, and with a fertilization treatment. Bacterial communities were characterized using next generation sequencing of part of the nodA gene (i.e. common to the core rhizobial symbionts of rooibos), and part of the gyrB gene (i.e. common to all bacterial taxa). Ecological drift alone was a major driver of taxonomic turnover in the bacterial communities of root nodules (62.6% of gyrB communities). In contrast, the assembly of core rhizobial communities (genus Mesorhizobium) was driven by dispersal limitation in concert with drift (81.1% of nodA communities). This agrees with a scenario of rooibos-Mesorhizobium specificity in spatially separated subpopulations, and low host filtering of other bacteria colonizing root nodules in a stochastic manner.
Rhinovirus impairs the immune response of alveolar macrophages to facilitate Streptococcus pneumonia infection
Authors: Wei Huiping , Zhao Hui , Li Ruifang , Yang Feiyun , Wu Yan 
Published: May 2020

ABSTRACT
Pneumonia is one important cause of mortality in neonates. However, the mechanism remains still unclear. Viral infection greatly enhances the morbidity of Streptococcus pneumonia. In this study, we tried to understand how human rhinovirus (HRV) would accelerate Streptococcus pneumonia infection. Alveolar macrophages (AMs) were isolated from neonatal mice. Cytokine concentrations were detected using ELISA. The phagocytosis of Streptococcus pneumonia by AMs was indicated by immunofluorescence. Toll-like receptor 3 (TLR3) and CD68 expression in isolated AMs or infected mice were determined by western blot or immunochemistry. The mortality was explored using Kaplan–Meier analysis. HRV infection enhanced cytokine release by AMs, and decreased Streptococcus pneumonia-induced TNF-α, IL-1β and IL-6 release by AMs, while has no influence on IL-10 release. HRV infection impaired phagocytosis of Streptococcus pneumonia in AMs. Mechanically, HRV infection up-regulated TLR3 expression in AMs. Mortality and pneumococcal burden decreased in TLR3−/− neonatal mice and inflammation and phagocytosis were restored in TLR3−/− AMs. Neonatal rhinovirus impairs the immune response of alveolar macrophages to facilitate Streptococcus pneumonia infection via TLR3 signaling.
The Protector within: Comparative Genomics of APSE Phages across Aphids Reveals Rampant Recombination and Diverse Toxin Arsenals
Authors: Rouïl Jeff , Jousselin Emmanuelle , Coeur d’acier Armelle , Cruaud Corinne , Manzano-Marín Alejandro 
Published: May 2020

Abstract
Phages can fundamentally alter the physiology and metabolism of their hosts. Although these phages are ubiquitous in the bacterial world, they have seldom been described among endosymbiotic bacteria. One notable exception is the APSE phage that is found associated with the gammaproteobacterial Hamiltonella defensa, hosted by several insect species. This secondary facultative endosymbiont is not necessary for the survival of its hosts but can infect certain individuals or even whole populations. Its infection in aphids is often associated with protection against parasitoid wasps. This protective phenotype has actually been linked to the infection of the symbiont strain with an APSE, which carries a toxin cassette that varies among so-called “types.” In the present work, we seek to expand our understanding of the diversity of APSE phages as well as the relations of their Hamiltonella hosts. For this, we assembled and annotated the full genomes of 16 APSE phages infecting Hamiltonella symbionts across ten insect species. Molecular and phylogenetic analyses suggest that recombination has occurred repeatedly among lineages. Comparative genomics of the phage genomes revealed two variable regions that are useful for phage typing. Additionally, we find that mobile elements could play a role in the acquisition of new genes in the toxin cassette. Altogether, we provide an unprecedented view of APSE diversity and their genome evolution across aphids. This genomic investigation will provide a valuable resource for the design and interpretation of experiments aiming at understanding the protective phenotype these phages confer to their insect hosts.
Magnitude distribution complexity and variation at The Geysers geothermal field
Authors: Leptokaropoulos Konstantinos 
Published: May 2020

SUMMARYEarthquake magnitude (size) distribution is a major component required for seismic hazard assessment and therefore, the accurate determination of its functional shape and variation is a task of utmost importance. Although often considered as stationary, the magnitude distribution at particular sites may significantly vary over time and space. In this study, the well-known Gutenberg–Richter (GR) law, which is widely assumed to describe earthquake magnitude distribution, is tested for a case study of seismicity induced by fluid injection at The Geysers (CA, USA) geothermal field. Statistical tests are developed and applied in order to characterize the magnitude distribution of a high quality catalogue comprising seismicity directly associated with two injection wells, at the north western part of The Geysers. The events size distribution variation is investigated with respect to spatial, temporal, fluid injection and magnitude cut-off criteria. A thorough spatio-temporal analysis is performed for defining seismicity Clusters demonstrating characteristic magnitude distributions which significantly differ from the ones of the nearby Clusters. The magnitude distributions of the entire seismic population as well as of the individual Clusters are tested for their complexity in terms of exponentiality, multimodal and multibump structure. Then, the Clusters identified are further processed and their characteristics are determined in connection to injection rate fluctuations. The results of the analysis clearly indicate that the entire magnitude distribution is definitely complex and non-exponential, whereas subsequent periods demonstrating significantly diverse magnitude distributions are identified. The regional seismicity population is divided into three major families, for one of which exponentiality of magnitude distribution is clearly rejected, whereas for the other two the GR law b-value is directly proportional to fluid injection. In addition, the b-values of these Families seem to be significantly magnitude dependent, a fact that is of major importance for seismic hazard assessment implementations. To conclude, it is strongly suggested that magnitude exponentiality must be tested before proceeding to any b-value calculations, particularly in anthropogenic seismicity cases where complex and time changeable processes take place.