bluuebunny/crossref_metadata_2025_split huggingface dataset:
Schizophrenia Risk ZNF804A Interacts with its Associated Proteins to Modulate Dendritic Morphology and Synaptic Development
Authors: Dong Fengping , Mao Joseph , Chen Miranda , Yoon Joy , Mao Yingwei 
Published: November 2020

Abstract
Schizophrenia (SZ) is a devastating brain disease that affect about 1% of world population. Among the top genetic associations, zinc finger protein 804A (ZNF804A) gene encodes a zinc finger protein, associated with SZ and biolar disorder (BD). Copy number variants (CNVs) of ZNF804A have been observed in patients with autism spectrum disorders (ASDs), anxiety disorder, and BD, suggesting that ZNF804A is a dosage sensitive gene for brain function. However, its molecular functions have not been fully determined. Our previous interactomic study revealed that ZNF804A interacts with multiple proteins to control protein translation and neural development. ZNF804A is localized in the cytoplasm and neurites in the human cortex and is expressed in various types of neurons, including pyramidal, dopaminergic, GABAergic, and Purkinje neurons in mouse brain. To further examine the effect of gene dosage of ZNF804A on neurite morphology, both knockdown and overexpression of ZNF804A in primary neuronal cells significantly attenuates dendritic morphology and synaptogenesis. To determine the effectors mediating these phenotypes, interestingly, three binding proteins of ZNF804A, galectin 1 (LGALS1), fasciculation and elongation protein zeta 1 (FEZ1) and ribosomal protein SA (RPSA), show different effects on reversing the deficits. LGALS1 and FEZ1 stimulate neurite outgrowth at basal level but RPSA shows no effect. Intriguingly, FEZ1 and RPSA but not LGALS1, can ameliorate ZNF804A-mediated dendritic abnormalities. Thus, our results uncover a critical post-mitotic role of ZNF804A in neurite and synaptic development relevant for neurodevelopmental pathologies.
Dissecting Immune Cell Stat Regulation Network Reveals Biomarkers to Predict ICB Therapy Responders in Melanoma
Authors: Wang Jingwen , Li Feng , Xu Yanjun , Zheng Xuan , Zhang Chunlong , Hu Congxue , Xu Yingqi , Mi Wanqi , Li Xia , Zhang Yunpeng 
Published: November 2020

Abstract
BackgroundImmunotherapy is a revolutionary strategy in cancer therapy，but the resistance of which is one of the important challenges. Detecting the regulation of immune cells and biomarkers concerning immune checkpoint blockade (ICB) therapy is great significance.MethodsHere, we investigated the CD45+ immune cell regulation networks and immunotherapy response features by integrating biological pathway data and single cell sequencing data in metastatic melanoma with or without ICB therapy. First, we constructed regulation networks for 11 predefined CD45+ cell stats in context with biology pathway. Furthermore, we identified differently expressed genes between responders and non-responders. Finally, we trained and validated a logistics regression model based on ligands and receptors in the regulation network to predict ICB therapy response. ResultsWe discovered the regulation of genes across various immune cell stats. Functional analysis indicated that these stat-specific networks consensually enriched in immune response corrected pathways and highlighted antigen processing and presentation as a core pathway in immune cell regulation. Furthermore, some famous ligands and receptors like SIRPA, CD14, IL2, HLA-G, ITGAM and CD247 were differently expression between cells of responders and non-responders before and (or) during course of treatment. Several gene sets containing differently expressed ligands and receptors performed accuracy prediction with AUCs in validation datasets above 0.7 suggesting that they may be server as biomarkers for predicting immunotherapy response.Conclusions﻿In summary, our study provided single cell-based regulation network in context of melanoma model with or without ICB therapy. Our analysis revealed several gene sets consist of therapy response biomarkers which is efficient for construction of ICB therapy response classifier.
miR-22/TET2/p53-Feedback Loop Regulates Cell Proliferation and Apoptosis of k562 cells
Authors: Yao Hongxia , Fu Xiangjun , Chen Yueqing , Duan Mengling , Guo Li , Chen Wenting , Pan Yanping , Ding Yipeng 
Published: November 2020

Abstract
BackgroundChronic myeloid leukemia (CML) is a major global health threat due to its low cure rate and high fatality rate. Studies have reported that miR-22, TET2, and p53 play a vital role in the progression of leukemia. However, it is unclear whether there is feedback regulation between them.MethodsTransfection efficiency of miR-22 and TET2 was detected by qRT-PCR. CCK-8 assay was applied to measure the proliferation ability of K562 cells. Flow cytometry was used for evaluation the cell cycle and apoptosis rate of K562 cells after transfection. Moreover, the interaction between miR-22, TET2 and P53 was analyzed by luciferase reporter gene assay.ResultsExperiments indicated that the TET2 expression was decreased and apoptosis was increased in the miR-22 mimic group, and cell cycle was arrested in G0/G1phase, the proliferation was markedly inhibited. Meanwhile we found that TET2 can affect the expression of p53. Then, we directly proved p53 inhibition miR-22 transcription, whereas miR-22 as a transcriptional repressor, TET2 expression is negatively regulated to form a feedback loop.ConclusionsmiR-22, TET2 and p53 can form a feedback loop and thus affect cell proliferation and apoptosis of chronic myeloid leukemia cells.
Schirmer strip and conjunctival swab for viral detection on the ocular surface of adults: a scoping review
Authors: Sabage Luís Expedito , Mazzo Alessandra , Sabage Josmar , Olivo Taylor Endrigo Toscano , Lourençone Luiz Fernando Manzoni 
Published: November 2020

Abstract
Purpose During the COVID-19 pandemic, one of the biggest challenges is accurate diagnosis, and it is known that, in some cases, ocular manifestations are one of the first symptoms. In this context, this study has the objective of raising scientific evidence that highlights the use of Schirmer strips and conjunctival swabs as a method of sample collection for viral analysis to support future research on this theme. Methods A literature search was developed in the PubMed, Web of Science and BVS databases and followed the Scoping Review protocol defined by Joana Brigs Institute (JBI) after the guiding question “Is it possible to detect viruses on the ocular surface with Schirmer Test and/or conjunctival swab?”. Results A total of 418 studies were identified, and after discerning analysis, 36 studies published in English were selected. Three researchers analyzed studies after virus research, collection methods, and sample analysis. Publications were mainly on adenovirus, herpes simplex virus and SARS-CoV-2, but there is evidence of ocular detection on more viruses. Discussion Studies have generally been conducted to understand viral infection, to develop accurate diagnostic methods and to follow the patient’s response to treatment. Most studies were developed on a small number of patients and lacked clear definitions of collection time and viral persistence since the onset of diseases. Viruses can be detected on the ocular surface through the analysis of Schirmer strips and conjunctival swabs. However, additional studies with larger populations and time permanence are necessary to develop more assertive conclusions on the theme.
Exploring eating and exercise-related indicators during COVID-19 quarantine: Concerns and routine changes in women with different BMI
Authors: Coimbra Maria , Paixão Carolina , Ferreira Cláudia 
Published: November 2020

Abstract
Purpose. The present study aimed to understand how quarantine associated to COVID-19 was experienced by women from different BMI groups (underweight, normal weight, pre-obesity, and obesity), by exploring the changes introduced in eating and exercise routines and the differences between BMI groups regarding shape concern, weight concern and binge eating behaviours reported during this time.Methods. This study comprised 580 women aged between 18 and 65 years old, who completed self-report measures, during the mandatory confinement in Portugal due to COVID-19. The total sample was divided into four groups, according to their BMI.Results. Results revealed that overall, there were no significant differences between the groups regarding the eating and exercise routine changes introduced during quarantine. However, significant differences between the four groups were found in the reported body and eating-related difficulties (shape concern, weight concern and binge eating behaviours), with the pre-obesity and obesity groups presenting significant higher levels. Conclusions. Our study suggests that, even though the changes introduced to the eating and exercise routine are independent from BMI, the pre-obesity and obesity group represent a major vulnerability group for the development of eating disorders. We hope this study allows professionals to understand the need to develop different guidelines and strategies for the pre-obesity and obesity female population during the deconfinement period. Level of evidence. Level V - Descriptive study.
Effectiveness of mobile applications in diabetic patients’ healthy lifestyles: review of systematic reviews.
Authors: Carrera Francisco Jesús Represas , Ques Ángel Alfredo Martínez , Fontán Ana Clavería 
Published: November 2020

Abstract
Background: Diabetes mellitus (DM) is currently a major public health problem worldwide. It is traditionally approached by a clinical inpatient relationship between patients and healthcare professionals. However, the rise in of the use of new technologies, particularly mobile applications, is revolutionizing the traditional healthcare model with the introduction of telehealthcare.Objective: (1) Examine the mobile applications that address lifestyles to improve the metabolic control of adult patients with Diabetes Mellitus. (2) Describe the characteristics of the used mobile applications, identify the healthy lifestyles they target, and describe any of their adverse effects.Methods: Review of systematic reviews following Cochrane Collaboration and Joanna Briggs Institute guidelines. We included studies that used any mobile application to help patients improve DM self-management by focusing on healthy lifestyles. Studies needed to include a control group receiving regular care without using mobile devices. In May 2018, Medline, Embase, Cochrane, LILACS, PsychINFO, Cinahl and Science Direct were searched, updated in May 2019. The methodological quality of the studies was assessed by the Amstar-2 tool.Results: Seven systematic reviews of 798 articles were initially selected for the analysis. Interventions lasted 1-12 months. Twenty-three different mobile applications were identified. They were all related to eating and physical activity. Significant changes were found in HbA1c values, body weight and BMI, but no clear improvement was observed in others like lipid profile, quality of life or blood pressure. No significant adverse effects were identified.Conclusions: Clearly evidence appeared for using mobile applications to improve glycemic control in diabetic patients in the short term, but not for long-term benefits. Thus carrying out further studies is necessary to learn about the long-term effectiveness of mobile applications to promote DM patients’ healthy lifestyles. PROSPERO Register: CRD42019133685
Comparative analysis, applications, and interpretation of electronic health record-based stroke phenotyping methods
Authors: Thangaraj Phyllis , Kummer Benjamin R , Lorberbaum Tal , Elkind Mitchell S.V. , Tatonetti Nicholas P 
Published: November 2020

Abstract
Background: Accurate identification of acute ischemic stroke (AIS) patient cohorts is essential for a wide range of clinical investigations. Automated phenotyping methods that leverage electronic health records (EHRs) represent a fundamentally new approach cohort identification without current laborious and ungeneralizable generation of phenotyping algorithms. We systematically compared and evaluated the ability of machine learning algorithms and case-control combinations to phenotype acute ischemic stroke patients using data from an EHR.Materials and Methods: Using structured patient data from the EHR at a tertiary-care hospital system, we built and evaluated machine learning models to identify patients with AIS based on 75 different case-control and classifier combinations. We then estimated the prevalence of AIS patients across the EHR. Finally, we externally validated the ability of the models to detect AIS patients without AIS diagnosis codes using the UK Biobank.Results: Across all models, we found that the mean AUROC for detecting AIS was 0.963±0.0520 and average precision score 0.790±0.196 with minimal feature processing. Classifiers trained with cases with AIS diagnosis codes and controls with no cerebrovascular disease codes had the best average F1 score (0.832±0.0383). In the external validation, we found that the top probabilities from a model-predicted AIS cohort were significantly enriched for AIS patients without AIS diagnosis codes (60-150 fold over expected). Conclusions: Our findings support machine learning algorithms as a generalizable way to accurately identify AIS patients without using process-intensive manual feature curation. When a set of AIS patients is unavailable, diagnosis codes may be used to train classifier models.
The pathological features of hip abductor tendon tears – a cadaveric study
Authors: Zhu Mark , Smith Brittany , Krishna Sanjeev , Musson David S. , Riordan Peter R. , McGlashan Sue R. , Cornish Jillian , Munro Jacob T. 
Published: November 2020

Abstract
Background: The hip abductors are crucial in maintaining pelvic stability. Tears in these tendons are common and often debilitating. There is uncertainty regarding both the histological and macroscopic features of hip abductor tears. This study aims to clarify both the macroscopic and microscopic features of the tendon and enthesis in hip abductor tendon tears.Methods: Thirty-six cadavers with an average age of 81 were dissected, and the hip abductor mechanisms removed en-bloc. The presence, location and size of the tears were recorded and analysed. The samples were processed into histological blocks and viewed using both transmitted and polarised light. Tendon histology was graded using the modified Movin’s score in three sections (deep, middle and superficial layers) and the enthesis graded separately using 5-point criteria. Analysis of variance was used to confirm histological features associated with tears.Results: Tears were found in 24 of 36 samples (67%). The most common finding was an isolated tear in the gluteus minimus (46%), followed by concurrent gluteus medius and gluteus minimus tears (33%). Histology revealed significantly more degeneration in both the tendon (p=0.0005) and enthesis (p=0.0011) when tears were present. Furthermore, these changes were concentrated in the deeper layers of the tendon (p=0.0002) and enthesis (p=0.003).Conclusion: This study demonstrated degeneration as the primary pathology underlying hip abductor tendon tears. Degenerative changes occur in both the tendon and enthesis, with the deeper layers predominantly affected. These findings are important for guiding surgical repair techniques and to aid the development of novel materials and biologics.
Prognostic significance of preoperative neutrophil-to-lymphocyte ratio in papillary renal cell carcinoma patients after receiving curative surgery based on a retrospective cohort
Authors: Zhang Zhilei , Yu Yongbo , Zheng Jilu , Zhang Mingxin , Niu Haitao 
Published: November 2020

Abstract
Background: Inflammatory response biomarkers have been studied as promising prognostic factors in renal cell carcinoma (RCC), but few studies have focused on papillary renal cell carcinoma (PRCC). This study was performed to evaluate the prognostic value of the preoperative neutrophil-to-lymphocyte ratio (NLR) in PRCC patients. Methods: In total, 90122 postoperative PRCC patients selected from 284 366 non-clear cell renal cell carcinoma (nccRCC) patients were enrolled from our institution between 2012 and 20192020. The optimal cutoff value of the NLR was assessed by receiver operating characteristic (ROC) curve analysis, and the Kaplan–Meier method and Cox's proportional hazards regression models were performed to analyze the association of the NLR with overall survival (OS). In addition, the potential of tumor-node-metastasis (TNM) stage, the NLR and an NLR-TNM system to predict survival were compared with ROC curves. Results: A threshold value of 2.39 for the NLR for OS analysis was determined by ROC curve analysis. An NLR≥2.39 was associated with a more advanced TNM stage (P<0.001) and larger tumors (P<0.05) than a low NLR, as well as pathological subtype Ⅱ (P<0.05), and the patients with a high NLR also exhibited significantly worse overall survival outcomes (P<0.05). The NLR was determined to be a significant independent prognostic indicator by univariable and multivariable analyses (HR = 5.557, P<0.05). Furthermore, TNM stage and the NLR were integrated, and the area under the curve (AUC) of for the NLR-TNM system was larger than that of for the TNM system when predicting overall survival (0.839 VS 0.731, P=0.036). Conclusion: A high preoperative NLR was associated with poor clinical and pathologic parameters in patients with PRCC; moreover, the NLR was also an independent prognostic factor for the OS of patients with PRCC. The NLR-TNM system, which was a model that integrated the NLR with TNM staging, could improve the ability to predict overall survival.
Parecoxib alleviates the inflammatory effect of leukocyte-rich platelet-rich plasma in normal rabbit tendons
Authors: Zhou Ming , Wang Ning , Wang Gang , Jia Zishan , Qi Xiaolei 
Published: November 2020

Abstract
Background: Platelet-rich plasma (PRP) is widely used to treat tendon injuries. Its therapeutic effect varies depending on the different cell components, and white blood cells (WBCs) may play an important role in this phenomenon. The purpose of this study was to evaluate how PRP with different concentrations of WBCs affect normal rabbit tendon and assess whether non-steroidal anti-inflammatory drugs (NSAIDs) can suppress the catabolic effects of WBCs.Methods: Sixteen adult New Zealand White rabbits were used. Blood samples were collected from each rabbit, and PRP was extracted following two different protocols to obtain leukocyte-poor PRP (LP-PRP) and leukocyte-rich PRP (LR-PRP). LP-PRP or LR-PRP was injected into the patellar tendon of each rabbit, while normal saline (NS) was injected as control. In LR-PRP + NSAID group, Parecoxib was administered after LR-PRP injection. For each group, 2 rabbits were euthanatized at day 5 and 14. The patellar tendons were collected and stained with hematoxylin and eosin. A semi-quantitative approach was used to assess the inflammatory response and tendon destruction based on the evaluation of the WBCs, vascularization, fiber structure, and fibrosis.Results: The LR-PRP group exhibited a higher total tendon score than the LP-PRP group at day 5 after PRP injection, but there was no significant difference between the two groups at day 14. For the NSAID group, the tendon score was lower than that of the LR-PRP group both at day 5 and 14.Conclusion: LR-PRP can promote a higher inflammatory response than LP-PRP in the normal rabbit patellar tendon, and this effect can be suppressed by NSAIDs.
Investigation of Awareness and Anxiety Levels of Pregnant Women During Pandemic Process. A Multi-Centric Descriptive Cross-Sectional Survey Study in Istanbul, Turkey
Authors: Palalioglu Rabia Merve , Karadeniz Ozan , Aytok Gokce Ipek , Palalioglu Batuhan , Koyan Gizem Nur , Erbiyik Halil Ibrahim , Muhcu Murat 
Published: November 2020

Abstract
Background:COVID-19 pandemic is an important and urgent public health problem. It is currently unknown that how pregnant women deal with the Coronavirus disease and its results. The aim of this study is to evaluate the psychological impact of the coronavirus pandemic on pregnant women and to determine whether pregnant women have sufficient knowledge and awareness for a healthy antenatal process.Methods: This prospective study was conducted at two centers. Regardless of the gestational age, a questionnaire was distributed to 1003 pregnant women in total, from 51 original questions that investigate the pregnant women’s demographic data, history of contact with COVID-19-positive patients, knowledge and concerns about COVID-19, precautions. 526 participants were included in the study. The questionnaire was delivered using the QR code method. The questionnaires were answered online by participants via SurveyMonkey in order to minimize contamination due to the pandemic.Results: The period when anxiety was highest was the 2nd trimester, whereas women in the 1st trimester had the lowest level of anxiety. High levels of awareness were observed in patients with heart disease, but patients with diabetes mellitus had a high level of anxiety. There was a statistically significant correlation between anxiety and awareness scores of the coronavirus disease.Conclusion:It is important to maintain the mental and physical health of pregnant women, who are in a more delicate condition than other individuals in the society. In this regard, healthcare professionals have important duties such as taking necessary precautions and explaining the seriousness of the situation to pregnant women.
Developing a scoring tool to estimate the risk of deterioration for normotensive patients with acute pulmonary embolism on admission&nbsp;
Authors: Gao Yizhuo , Ji Chao , Zhao Hongyu , Han Jun , Shen Haitao , Jia Dong 
Published: November 2020

Abstract
Background: It is important to identify deterioration in normotensive patients with acute pulmonary embolism (PE). This study aimed to develop a tool for predicting deterioration among normotensive patients with acute PE on admission.Methods: Clinical, laboratory, and computed tomography parameters were retrospectively collected for normotensive patients with acute PE who were treated at a Chinese center from January 2011 to May 2020 on admission into the hospital. The endpoint of the deterioration was any adverse outcome within 30 days. Eligible patients were randomized 2:1 to derivation and validation cohorts, and a nomogram was developed and validated by the aforementioned cohorts, respectively. The areas under the curves (AUCs) with 95% confidence intervals (CIs) were calculated. A risk-scoring tool for predicting deterioration was applied as a web-based calculator.Results: The 845 eligible patients (420 men, 425 women) had an average age of 60.05±15.43 years. Adverse outcomes were identified for 81 patients (9.6%). The nomogram for adverse outcomes included heart rate, systolic pressure, N-terminal-pro brain natriuretic peptide, and ventricle/atrial diameter ratios at 4-chamber view, which provided AUC values of 0.925 in the derivation cohort (95% CI: 0.900–0.946, p<0.001) and 0.900 in the validation cohort (95% CI: 0.883–0.948, p<0.001). A risk-scoring tool was published as a web-based calculator (https://gaoyzcmu.shinyapps.io/APE9AD/).Conclusions: We developed a web-based scoring tool that may help predict deterioration in normotensive patients with acute PE.