bluuebunny/crossref_metadata_2025_split huggingface dataset:
Curdione induces G2/M phage arrest, apoptosis and autophagy via COX2 mediate IDO1 expression through PKCδ/ GSK3β/β-catenin pathway in human uterine leiomyosarcoma
Authors: wei Chao , Li Donghua , Wang Wenna , Liu yu , Qiu tiantian 
Published: August 2020

Abstract
Background Curdione, one of active ingredients of a traditional Chinese herb medicine-Curcuma zedoary, has been reported with beneficial therapeutic effects in lots of cancer types. However, the potential anti-cancer effect in uterine leiomyosarcoma (uLMS) and the underlying mechanisms are still unclear.Methods The aim of this study was to explore the potential effect and mechanisms of curdione on uLMS in vitro and vivo with uLMS cell lines and mouse xenograft tumor model respectively.Results Curdione triggered anti-proliferation effect in uLMS cell lines by inducing cell cycle arrest at G2/M phase, caspase-mediated cell apoptosis, and pro-death cell autophagy. Indoleamine-2,3-dioxygenase-1 (IDO1), which was dependent on cyclooxygenase-2 (COX2), mediated the anti-proliferation effect of curdione. Curdione down-regulated IDO1 expression and promoted the dephosphorylation of protein kinase c (PKC), glycogen synthase kinase-3 beta (GSK3β) and β-catenin in cell lines. PKC/GSK3β/β-catenin pathway responsible for constitutive IDO1 expression in uLMS. COX2 mediate the promotion effect of curdione on the PKC/GSK3β/β catenin pathway activity, which was detected by COX2 inhibitor, and further confirmed by COX2 siRNA and COX2 overexpression. The pathway activity was inhibited by COX2-siRNA and enhanced by COX2 overexpression. In turn, the pathway inhibitor suppressed IDO1 expression. The anti-proliferation effect of curdione on uterine leiomyosarcoma were further confirmed in vivo. Echoing to the results in vitro, the underline mechanism involved in COX2-mediated IDO1 down-regulation via PKCδ/GSK3β/β-catenin pathway.Conclusion COX2-mediated IDO1 down-regulation via PKCδ/GSK3β/β-catenin pathway involved in the anti-proliferation effect of curdione on uterine leiomyosarcoma in vitro and vivo.
Proteomic Analysis to Explore the Effect of Zishen Jiangtang Pills on Diabetic Osteoporosis Rats
Authors: Chu Shufang , Liu Deliang , Zhao Hengxia , Shao Mumin , Chen Jianping , Liu Xuemei , Qu Xin , Li Zengying , Li Jinhua , Li Huilin 
Published: August 2020

Abstract
Context Zishen Jiangtang Pill (ZJP) is a Chinese herbal compound that could play a positive role in the treatment of Diabetic osteoporosis (DOP) by regulating glucose and bone metabolism. However, the specific mechanisms are still unclear.Background To explore the effect and mechanism of ZJP on DOP rats by proteomic analysis.Materials and methods After the establishment of diabetes model by 0.2% STZ, 40 Wistar rats were equally divided into normal group, model group (diabetic rats), high dose group (3.0 g/kg/d ZJP) and low dose group (1.5 g/kg/d ZJP), and received 3 months of treatment. Histological changes in bone and pancreas tissues were observed by Hematoxylin and eosin staining, electron microscopy, and immunofluorescence. Proteomic and bioinformatics analyses were performed to identify the differentially expressed proteins. The fingerprint and active ingredients of ZJP were identified via HPLC.Results Compared with the model group, ZJP could rescue the weight, fasting blood glucose and fasting insulin of rats in both high and low dose group. ZJP could also improve the micro-structures of pancreatic islet cells, and bone mass, trabecular and marrow cavities in DOP rats. Bioinformatic analysis suggested that ZJP might influence DOP via multiple pathways, mainly including ribosomes, vitamin digestion and absorption, and fat digestion and absorption. The primary active ingredients, including notoginsenoside R1, ginsenoside Rg1, ginsenoside Re, icariin, and ginsenoside Rb1 were detected.Conclusion ZJP could significantly improve the histomorphology and ultrastructure of bone and islets tissues, and might serve as an effective alternative medicine for the treatment of DOP.
Elevated level of N-terminal pro B-type natriuretic peptide is associated with myocardial fibrosis in hypertrophic cardiomyopathy patients with preserved ejection fraction
Authors: Shi Heshui , Li Yumin , Liu Jia , Cao Yukun , Zhou Xiaoyue , Han Xiaoyu , Gu Jin , Shao Guozhu , Han Tongtong , Cui Yue 
Published: August 2020

Abstract
Background: Myocardial fibrosis assessed by late gadolinium enhancement (LGE) on cardiovascular magnetic resonance (CMR) has been reported to be significantly correlated with cardiovascular outcomes in hypertrophic cardiomyopathy (HCM) patients. However, data regarding non-invasive markers for detecting myocardial fibrosis were inconsistent and, not systematically evaluated in HCM patients with preserved ejection fraction (EF).Methods: In this study, 86 HCM patients with preserved EF and 33 controls were enrolled. The left ventricular function, end-diastolic maximum wall thickness (MWT), global systolic strains and extent of LGE (% LGE) were assessed. The biochemical indices were also recorded before the CMR examination.Results: Serum high-sensitivity cardiac troponin I (hs-cTnI) and N-terminal pro b-type natriuretic peptide (Nt-proBNP) levels were elevated in LGE-positive patients compared with LGE-negative patients (p < 0.05 for all). The LGE-positive patients had lower global longitudinal (GLS) and circumferential (GCS) strains than the LGE-negative group and the healthy controls (p < 0.05 for all). The LGE% was independently associated with the Nt-proBNP levels (standardized β = 0.627, p < 0.001), beta-blocker treatment (standardized β = -0.372, p = 0.01), MWT (standardized β = 0.481, p = 0.001) and GCS (standardized β = 0.406, p = 0.013). In the receiver operating characteristic (ROC) curve analysis, the combined parameters of Nt-proBNP ≥ 108 pg/mL and MWT ≥ 17.3 mm had good diagnostic performance for LGE, with a specificity of 81.3% and sensitivity of 70.0%.Conclusions: This study suggests that Nt-proBNP may be a potential biomarker associated with LGE% and, combined with MWT, was useful in detecting myocardial fibrosis in HCM patients with preserved EF. Additionally, LV GCS may be a more sensitive indicator for reflecting the presence of myocardial fibrosis than GLS.
Molecular and Pathotypic Characterization of Fowl Adenovirus Associated with Inclusion Body Hepatitis in Indian Chickens
Authors: Shinde Dasharath B. , Thormoth Anil L , Koratkar Santosh S. , Sharma Neeti , Rajguru Ashok , Rale Vinay , Wagh Prasad , Prajitno Teguh Y. , Tongaonkar Satish S. 
Published: August 2020

Abstract
Inclusion body hepatitis (IBH) is a disease of chickens characterized by acute mortality with severe anemia caused by fowl adenoviruses (FAdV). IBH is one of the most important re-emerging diseases world-wide. In India earlier IBH/ Hydropericardium Syndrome (HPS) outbreaks were mainly due to FAdV) type 4; however, in recent outbreaks, other serotypes are involved, despite regular vaccination in breeders and young chicks. The aim of the present study was isolation and molecular characterization of FAdV associated with IBH in India. A total of 193 liver samples of IBH suspected broiler chickens were collected from different regions of India. Liver samples were initially screened by histopathology and further, a total of 127 samples which microscopically showed basophilic, intra-nuclear inclusion bodies were processed for FAdV detection. A total of 69 samples were found to be positive for Hexon gene in PCR, of which 28 were sequenced. These samples showed more than 94% sequence homology with FAdV2 and 97% with FAdV11, which was confirmed by Restriction Enzyme Analysis (REA). Tissue Culture Infective Dose 50 (TCID50) and Egg Infective Dose50 (EID50) titer of isolated FAdV was found to be 106.5/ml. Pathogenicity study in SPF chicks showed 100% mortality up to Post Infection Day (PID)-6. These findings conclude that the prevalence of FAdV-11 is currently causing IBH outbreaks India. However, further genetic and pathogenic analysis of FAdV is required, which would provide useful information for the development of the efficient commercial IBH vaccine.
Association of rs3746444 in MiRNA-499 and rs3787016 in Long Non-coding RNAs POLR2E with Prostate Cancer: an updated meta-analysis and systematic review
Authors: Liu Xuebin , Liu Yan , Wang Zhaozhang , Li Yuwu , Li Yubin , Zou Zihao , Zhong Jianfeng , Deng Nan , Zhou Gaorui , Liu Ping 
Published: August 2020

Abstract
Background

It has been proved that a significant proportion of prostate cancer diagnoses may be associated with a strong hereditary component. Micro-RNAs and Long Non-coding RNAs are a group of a noncoding small molecule, which play critical roles in signalling pathways, metabolism, apoptosis and cancer development. Exercising a meta-analysis to examine the relationship between rs3746444, rs3787016 and prostate cancer (PCa) risk was the main objective of our study.
Results

10 case-control studies were included, while 6 on rs3787016 and 4 on rs3746444. On the whole, the genotypes carrying the T allele, in which were verified an increased risk between rs3787016 and PCa risk.( T vs C: odds ratio(OR) = 1.802, 95% CI 1.015–3.198, P value of heterogeneity(PH) = 0.00%; CT vs CC: OR = 1.179,95% CI 1.091–1.275, PH = 0.41; TT vs CC: .OR = 1.418, 95% CI 1.229–1.636, PH= 0.961; TT + CT vs CC: OR = 1.216, 95% CI 1.129–1.310, PH= 0.408; TT vs CT + CC: OR = 1.328, 95%CI 1.159–1.521, PH = 0.987). A vital relevance was performed by this meta-analysis in three models between rs3746444 and PCa risk, in which a tendency of increased PCa risk could be found (C vs T: OR = 1.197, 95% CI 1.035–1.384, PH= 0.837; CC vs TT: OR = 1. 137, 95% CI 0.813–1.590, PH = 0.392; CC + CT vs TT: OR = 1.426, 95% CI 1.166–1.743, PH= 0.406.).
Conclusion

Our findings proposed that rs3746444 in MiRNA-499 and rs3787016 in Long Non-coding RNAs POLR2E polymorphisms increased the risk of developing PCa. Further functional studies are warranted to reveal the role of the polymorphism in carcinogenesis.
MiR-217 Promotes Osteogenic Differentiation of Periodontal Ligament Stem Cells Though EZH2-mediated Epigenetically Regulating Wnt Pathway
Authors: Jiang Hao , Jia Peizeng 
Published: August 2020

Abstract
Background: This study aimed to investigate the potential roles of miR-217 and EZH2 in the osteogenic differentiation of PDLSCs.Methods: The expression pattern of miR-217 and EZH2 in PDLSCs during osteogenesis was detected by qRT-PCR. Then gain-and loss-of-function was performed to confirm the roles of miR-217 and EZH2 in the osteogenic differentiation of PDLSCs. Alkaline phosphatase and alizarin red S staining were used to detect the activity of osteoblasts and mineral deposition. Western blot and qRT-PCR were performed to detected osteogenic markers to conform osteogenesis phenotype. Furthermore, dual luciferase reporter assay was conducted to analyze the binding of miR-217 to EZH2. Chromatin immunoprecipitation analysis were performed to explore the mechanism of EZH2 and miR-217 on Wnt pathway.Results: miR-217 was significantly upregulated during the osteogenic differentiation, whereas EZH2 was significantly downregulated. Moreover, knockdown of miR-217 and overexpression of EZH2 inhibited the ALP activity, ARS staining, and expression of osteogenic genes. Furthermore, overexpression of EZH2 partially reversed the effects of miR-217 overexpression on osteoblast differentiation. Finally, miR-217 overexpression promoted the expression of Wnt genes, resulting in the activation of the Wnt/β-catenin signaling pathway by targeting EZH2.Conclusion: Our results demonstrated that miR-217 is induced by osteogenic stimuli and promotes osteogenic differentiation partly by targeting the EZH2/Wnt1/β-Catenin signaling pathway. This study provides a novel understanding of the mechanisms of osteogenic differentiation, and suggests a potential method for promoting bone formation.
Clinical Application of the Spatial&nbsp;QRS-T Angle for the Prediction of Pulmonary Arterial Hypertension&nbsp;in Patients with Acute Pulmonary Embolism
Authors: Chang Qinghua , Li Changjun , Chai Wenshu , Fan Huangyuan , Zhang Chenchen 
Published: August 2020

Abstract
Background: The objective of our study was to assess the clinical application of the spatial QRS-T angle for the prediction of pulmonary arterial hypertension (PAH) in patients with acute pulmonary embolism.Methods: The study group of 162 patients with acute pulmonary embolism was stratiﬁed on the basis of color doppler echocardiographic ﬁndings into two subgroups: non-PAH group (n = 92) and PAH group (n = 70). Then PAH group patients were stratified into mild, moderate, and severe PAH group. Independent t-test was used to compare the indexes of non-PAH group and PAH group. One-way ANOVA was used to compare the indexes of mild, moderate, and severe PAH group. Pearson correlation analysis was used to evaluate the signiﬁcance of the correlation between acute pulmonary arterial systolic pressure (PASP) and spatial QRS-T angle.Results: The spatial QRS-T angle was larger in patients with PAH than those with non-PAH (109.010±30.970° VS 68.098±18.010°, P < 0.05). Signiﬁcant differences of spatial QRS-T angle values were found between patients with mild, moderate and severe PAH (73.714±15.716°, 104.790±20.675°, 139.57±23.196°, respectively, P < 0.05). There was a positively linear correlation between the spatial QRS-T angle and PASP in patients with PAH.Conclusions: Spatial QRS-T angle are potentially useful for differentiation between acute pulmonary embolism patients with PAH from those without PAH, and possibly also for predicting PASP in acute pulmonary embolism patients.
Multidrug-Resistant Microorganisms Associated with Urinary Tract Infections Following Orthopaedic Patients: A Laboratory-Based Analysis of 5239 Patients
Authors: Ziółkowski Grzegorz , Pawłowska Iwona , Stasiowski Michał , Jachowicz Estera , Bielecki Tomasz 
Published: August 2020

Abstract
Background: The risk of healthcare associated infections (HAI) in surgical wards remains closely related to the type of surgery and procedures performed on patients. They also condition the risk of various, apart from the most common, surgical site infections, forms of clinical infections, especially urinary tract infections (UTIs). Their occurrence in orthopedic patients is most often – in about 70%-80%, associated with the use of the bladder catheter in the perioperative period. The aim of this study was the epidemiological and microbiological analysis of UTIs following orthopedic patients, especially MDR in 2013-2015.Methods: The study was conducted in 38-bed Department of Orthopedic-Traumatic Surgery in Sosnowiec, Poland. 5 239 patients surgery included in the study, the urinary catheter utilization rate was 30.7 %. Laboratory based study using the UTI definition of the HAI-Net program, MDR was defined as resistant to at least one antibiotic from 2< antibiotics groups. The results were analyzed using the statistical package PQStat ver. 1.6.0.428 using the chi-square test (chi ^ 2) or Fisher's exact test.Results The UTI incidence was 3.2% (168 cases), CA-UTI incidence density was 9.6/1 000 catheter-days. The highest risk of UTI was found in patients aged 75 years and older. Gram-negative flora prevailed among microorganisms: 76.1%. Predominantly isolated Enterobacterales were: Escherichia coli, Klebsiella pneumoniae. Isolated microorganisms were fully sensitive to carbapenems. Gram-negative bacilli showed the lowest sensitivity to extended substrate spectrum penicillins and fluoroquinolones: 37-64%, trimethoprim-sulfamethoxazole: 50%; MDR prevalence: 24.4%.Conclusions The presented data indicate that UTI incidence is a significant problem in studied population, as well as antimicrobialresistance, especially to quinolones and extended spectrum cephalosporins, as first-line therapy remains a major challenge. To reduce the problem of high UTI incidence and MDR prevalence, the priority should be the reduction of UTI risk – which exceeded the expected values.
Behavioral Effects of Different Contraceptive Methods and HIV Acquisition: An Ancillary Study of the ECHO* Randomized Trial
Authors: Singata-Madliki Mandisa , Lawrie Theresa , Balakrishna Yusentha , d’Hellencourt Florence Carayon-Lefebvre , Hofmeyr George Justus 
Published: August 2020

Abstract
BackgroundThe ECHO trial randomised 7829 women to depot medroxyprogesterone acetate (DMPA-IM), the copper intrauterine device (IUD) and the levonorgestrel (LNG) implant (1:1:1) and found no clear difference in HIV incidence between these three groups. We have previously hypothesized that oligo-amenorrhoea induced by DMPA-IM may have a protective effect on HIV acquisition. The aim of this ancillary study was to assess the effects of DMPA-IM, the IUD and the LNG implant on menstrual symptoms and sexual behavior and to correlate these with HIV acquisition.Methods At the Effective Care Research Unit (ECRU) in South Africa, women already randomised to DMPA-IM, the copper IUD and the LNG implant (1:1:1) were asked to participate. Participants completed a 28-day symptom and behavior diary following their one-month ECHO trial visit and returning it at their 3-month follow-up visit. HIV acquisition data were retrieved from ECHO trial records.ResultsOf 487 women enrolled on the ancillary study, 390 (80%) completed their daily diary; 130, 133, and 127 received DMPA-IM, IUD, and LNG implant, respectively. Thirty-three (8·4%) of these women acquired HIV. Women on the progestin-only contraceptives were more likely to experience amenorrhoea, as expected, and were less likely to have intra-menstrual coitus than IUD users (p < 0·001 for DMPA-IM vs IUD and P = 0·002 for implant vs IUD). Overall coital frequency was highest and condom usage lowest among DMPA-IM users. Intra-menstrual coitus correlated positively, and duration of menstruation correlated negatively, with HIV acquisition, although these effects were not statistically significant (p=0·09 and p=0·079, respectively).ConclusionsFindings support the hypothesis that oligo-amenorrhoea and the associated reduced intra-menstrual coitus may mitigate the potential for an increased biological risk of HIV acquisition with DMPA-IM but more evidence is needed. Study registration number: PACTR201706001651380
Investigation of Microstructure and Dielectric Behavior of Bi2/3Cu3-xMgxTi4O12(x=0, 0.05, 0.1 and 0.2) Ceramics Synthesized by Semi-Wet Route
Authors: Rai Vishnu Shankar , Pandey Santosh , Kumar Vinod , Verma Manish Kumar , Kumar Atendra , Singh Shruti , Prajapati Dinesh , Mandal Prof. K.D. 
Published: August 2020

Abstract
In this manuscript, We have reported the synthesis and characterization of Mg-doped and undoped BCTO ceramic (Bi2/3Cu3-xMgxTi4O12, x=0,0.05,0.1,0.2) sintered at 1173 K for 8 h, which have been prepared by the semi-wet path. Single-phase formation of ceramic was approved by the XRD pattern. The Microstructural properties were studied by TEM and AFM. The samples were characterized by dielectric and impedance spectroscopic properties. The dielectric constant (εr) was calculated to be 3024 for BCTO ceramics at 423 K and 100 Hz. The tangent loss (tan δ) value was calculated to be 0.45 for BCTO ceramic at 423 K and 10 kHz. The internal Barrier Layer Capacitance (IBLC) mechanism was responsible for the high value of dielectric constant. XPS spectroscopy confirmed the oxidation state of the ceramic. It was observed from Impedance and modulus studies that there was the existence of the Maxwell-Wagner form of relaxation in the ceramics.
Examining the News Media Reaction to a National Sugary Beverage Tax in South Africa: A Quantitative Content Analysis.
Authors: Essman Michael , Stoltze Fernanda Mediano , Carpentier Francesca Dillman , Swart Elizabeth C , Taillie Lindsey Smith 
Published: August 2020

Abstract
Background: South Africa was the first sub-Saharan African country to implement a sugar-sweetened beverage (SSB) tax called the Health Promotion Levy (HPL) in April 2018. Given news media can increase public awareness and sway opinions, this study analyzed how the media represented the HPL, including expressions of support or challenge, topics associated with the levy, and stakeholder views of the HPL.Methods: We performed a quantitative content analysis of online South African news articles related to the HPL published between January 1, 2017 and June 30, 2019. We coded the presence or absence of mentions related to health and economic effects of the HPL and HPL support or opposition. Prevalence of these mentions, overall and by source (industry, government, academics, other), were analyzed with Pearson χ2 and post-hoc Fisher exact tests. Results: Across all articles, 81% mentioned health, and 65% mentioned economics topics. 54% of articles expressed support, 26% opposition, and 20% a balanced view of the HPL. All sources except industry expressed majority support for the HPL. Health reasons were the most common justifications for support, and economic harms were the most common justifications for opposition. Statements that sugar intake is not related to obesity, the HPL will not reduce SSB intake, and the HPL will cause industry or economic harm were all disproportionately high in industry sources (92%, 80%, and 81% vs 25% prevalence in total sample) (p<0.001). Statements that sugar intake is related to obesity and non-communicable diseases were disproportionately high in both government (46% and 54% vs 31% prevalence in total sample) (p<0.001) and academics (33% and 38% vs 25% prevalence in total sample) (p<0.05). Statements that the HPL will improve health and the HPL will reduce health care costs were disproportionately high in government (47% vs 31% prevalence in total sample) (p<0.001) and academics (44% vs 25% prevalence in total sample) (p<0.05), respectively. Conclusions: Industry expressed no support for the HPL, whereas academics, government, and other sources mainly expressed support. Future studies would be improved by linking news media exposure to SSB intake data to better understand the effects news media may have on individual behavior change.
Community drug distributors’ experiences and challenges faced in the implementation of community-directed treatment with ivermectin programme for onchocerciasis control in Ulanga, Tanzania
Authors: Mushi Vivian 
Published: August 2020

Abstract
Background: Community drug distributors (CDDs) have a crucial role in distributing ivermectin for onchocerciasis control and prevention. Their experiences and challenges faced in the implementation of the community-directed treatment with ivermectin (CDTI) programme could potentially affect coverage, consequently leading to persistent transmission. Therefore, this study aimed to explore CDDs experiences and challenges faced in the implementation of the CDTI programme in Ulanga, Tanzania.Methods: A qualitative study was conducted in Ulanga, Tanzania, in 2018. An in-depth interview guide was used to collect data from 5 CDDs above 18 years who were selected purposively. The analysis was performed using a thematic framework approach to generate codes, categories and themes.Results: Out of the five CDDs interviewed, two had 15 to 20 years of experience in the implementation of the CDTI programme, while the remaining CDDs had less than 10 years of experience. The main challenges faced by CDDs in the implementation of the CDTI programme include the geographical location of the hamlets (hard to reach hamlets), long distances between houses, low compliance of community members to medication due to fear of side effects experienced before and mistrust of methods of dose calculation, short time of drug distribution and absence of people from their households as the exercise was conducted when community members were involved in agricultural activities.Conclusions: The use of CDDs in the implementation of the CDTI programme has been successful despite the challenges mentioned. It is now opportune time to address the challenges that CDDs are facing in the implementation of the CDTI programme to ensure effective control of onchocerciasis in the district.