bluuebunny/crossref_metadata_2025_split huggingface dataset:
Brain Stimulation in Attention Deficits after Traumatic Brain Injury: A Literature Review and Feasibility Study
Authors: Boissonnault Ève , Higgins Johanne , LaGarde Geneviève , Barthélemy Dorothy , Lamarre Céline , Dagher Jehane H. 
Published: October 2020

Abstract
Background: After a traumatic brain injury, disturbances in the attentional processes have a direct negative effect on functional recovery and on return to complex activities. To date, there is no good attention remediation treatment available. The primary objective of this review and pilot study is to get a comprehensive analysis and to evaluate the feasibility of implementing a tDCS protocol to improve attention disorders in patients with mild complicated to severe subacute TBI, hospitalized in an inpatient rehabilitation facility. Our secondary objective is to extract preliminary data and observational information on participants’ response to treatment. Methods: Participants were recruited from a consecutive series of patients admitted to the TBI unit of a subspecialized regional rehabilitation center. They received a 20-minute tDCS stimulation 3 times a week for 3 weeks. A neuropsychological evaluation was performed before and after the intervention. We collected participants’ socio-demographic and clinical characteristics as well as information about satisfaction, tolerability and adverse effects.Results: 164 patients were admitted between September 2018 and January 2020. 158 were excluded and 6 patients with presumed attentional deficits were enrolled. None completed the protocol as intended. No major side effects occurred. Conclusion: Non-invasive brain neurostimulation is promising to enhance attention deficits in patients with TBI. Implementation of a tDCS protocol to fulfill this purpose in an intensive inpatient rehabilitation center has its limitations. We made recommendations to facilitate the implementation of similar projects in the future. Trial registration: ISRCTN, ISRCTN55243064. Registered 14 October 2020 - Retrospectively registered, http://www.isrctn.com/ISRCTN55243064.
Electric Signals Counterbalanced Posterior vs Anterior PTEN Signaling in Directed Migration of Dictyostelium
Authors: Song Bing , Gu Yu , Jiang Wenkai , Li Ying , Ayre Wayne Nishio , Liu Zhipeng , Yin Tao , Janetopolous Christopher , Iijima Miho , Devreotes Peter , Zhao Min 
Published: October 2020

Abstract
Background: Cells show directed migration response to electric signals, namely electrotaxis or galvanotaxis. PI3K and PTEN jointly play counterbalancing roles in this event via a bilateral regulation of PIP3 signaling. PI3K has been proved essential in anterior signaling of electrotaxing cells, whilst the role of PTEN remains elusive.Methods: Dictyostelium cells with different genetic backgrounds were treated with direct current electric signals to investigate the genetic regulation of electrotaxis.Results: We demonstrated that electric signals promoted PTEN phosphatase activity and asymmetrical translocation to the posterior plasma membrane of the electrotaxing cells. Electric stimulation produced a similar but delayed rear redistribution of myosin II, immediately before electrotaxis started. Actin polymerization is required for the asymmetric membrane translocation of PTEN and myosin. PTEN signaling is also responsible for the asymmetric anterior redistribution of PIP3 / F-actin, and a biased redistribution of pseudopod protrusion in the forwarding direction of electrotaxing cells. Conclusions: PTEN controls electrotaxis by coordinately regulating asymmetric redistribution of myosin to the posterior, and PIP3/F-actin to the anterior region of the directed migration cells.
Development and validation of a scale evaluating pregnant women's breastfeeding competency in third trimester: Breastfeeding Competency Scale(BCS)
Authors: Wu Yu , Wang Ying , Hu Jiazhen , Dang Yan , Zhang Yuanyuan , Qi Xiumei , Tian Qingxiu , Wang Aihua , Li Yunfeng 
Published: October 2020

Abstract
Background: Breastfeeding plays an important role in the early stages of humans and throughout the development process. Breastfeeding competency is a self-assessment of pregnant women's overall competency to breastfeeding which could predict behaviors of pregnant women’ breastfeeding. However, a valid and reliable scale to assess the breastfeeding competency has not yet been developed and validated. This study was designed to develop and validate an assessment scale designed to assess the pregnant women's breastfeeding competency in third trimester: Breastfeeding Competency Scale(BCS).Methods: The BCS was developed and validated over three phases between September 2018–September 2019 which include item statistics, exploratory factor analysis(EFA), content validation, internal consistency assessment, split-half reliability and confirmatory factor analysis.Results: Item statistics and exploratory factor analysis resulted in 38 items, 4 factors that explained 66.489% of total variance. The Cronbach’s α coefficient in total scale and 4 factors were 0.970, 0.960, 0.940, 0.822, 0.931 respectively. The split-half reliability of BCS was 0.894, 0.890. Confirmatory factor analytic model showed the 4-factor model matching the data well.Conclusions: The BCS was a new instrument with certain validity and reliability for assessing the breastfeeding competency of pregnant women in third trimester.
Minimally invasive closure of transthoracic ventricular septal defect: postoperative complications and risk factors
Authors: Ren Chunnian , Wu Chun , Pan Zhengxia , Li Yonggang 
Published: October 2020

Abstract
ObjectivesTo summarize and analyze the clinical characteristics of postoperative complications after minimally invasive closure of transthoracic ventricular septal defect, and to explore the risk factors for its occurrence.MethodsRetrospectively analyzed the clinical data of 209 patients underwent transthoracic ventricular septal defect closure performed in the Department of Cardiothoracic Surgery, Children’s Hospital of Chongqing Medical University from January 2018 to January 2020, obtained relevant clinical data from the electronic medical record system and summarized their postoperative complications. And used univariate logistics regression and multivariate logistics regression to analyze the risk factors of its occurrence.ResultsThe postoperative hospital stay of 27 patients was longer than 9 days. Residual shunt occurred in 33 patients recently after operation. One patient underwent surgical treatment again because of mechanical hemolysis after the operation. Two patients were re-operated 1 month and 10 months after surgery because of persistent moderate to severe aortic regurgitation. After surgery, 3 patients underwent pericardiocentesis due to a large amount of pericardial effusion, and 2 patients developed a new atrioventricular block after the operation. No other serious adverse events occurred. Multivariate logistic regression analysis showed that the size of VSD defect (OR: 1.494, 95% Cl: 1.108-2.013, P value: 0.008) was related to long postoperative hospitalization. The residual shunt is related to the size of the occluder (OR: 1.452, 95%Cl: 1.164-1.810, P value: 0.001). In the univariate logistics regression analysis, no risk factors related to serious adverse events were found.ConclusionsThe minimally invasive closure of transthoracic ventricular septal defect is very effective, with no mortality and low incidence of serious adverse events after surgery. The size of the defect is related to the long postoperative hospitalization, and the size of the occluder is related to the residual shunt in the early postoperative period. No risk factors related to the occurrence of serious adverse events after the operation were found.
Meta-Analysis of Economic Evaluation Studies: Data Harmonisation and Methodological Issues
Authors: Bagepally Bhavani Shankara , Chaikledkaew Usa , Chaiyakunapruk Nathorn , Attia John , Thakkinstian Ammarin 
Published: October 2020

Abstract
In the context of ever-growing health expenditure and limited resources, economic evaluations aid in making evidence-informed policy decisions. Cost utility analyses (CUA) are often used in this context, but limitations include pairwise contrasts, missing contrasts, and different sources or quality of data. Synthesis of CUA data from multiple studies is therefore desirable to assist policy makers, but there are many challenging methodological issues including: inconsistent reporting of results using different economic parameters, and multiple sources of heterogeneity including: setting, time horizon, perspective, modelling approaches and assumptions, currency, willingness to pay (WTP) threshold, level of country income, and input parameters. In this paper, we provide a step by step description of the methods for data harmonisation and synthesis of aggregated data from CUA studies, as well as a framework for handling heterogeneity; we demonstrate these methods using the example of agents for type 2 diabetes. These meta-analytic methods for the synthesis of economic evidence synthesis should be useful for policy makers.
Adapting the Wheelchair Skills Program for Pediatric Rehabilitation: Recommendations from Key Stakeholders
Authors: Daoust Geneviève , Rushton Paula W. , Racine Marissa , Leduc Karolann , Assila Najoua , Demers Louise 
Published: October 2020

Abstract
Background: Backed by over 20 years of research development, the Wheelchair Skills Program (WSP) has proven to be a safe and effective program to improving wheelchair skills for adult wheelchair users. However, evidence is lacking for the pediatric population, which may help to explain the limited use of the WSP in pediatric settings. While additional evidence specific to the pediatric population is needed, concurrent implementation of the WSP into pediatric clinical practice is equally prudent to allow those users to benefit from the years of accumulated WSP evidence. To facilitate implementation of evidence-based programs into practice, adaptation is also often required to improve the fit between the program and the local context. Therefore, the objective of this study was to understand what adaptations, if any, are required for the Wheelchair Skills Program (WSP) to be implementable in a pediatric setting. Methods: A deductive qualitative descriptive study design was used, guided by the Consolidated Framework for Implementation Research (CFIR). Occupational Therapist (OTs) from a pediatric rehabilitation center and two specialized schools in Montreal, Canada were invited to participate in a 90-min focus group. The Framework Method was followed for the data analysis. Results: One focus group in each site (n=3) was conducted with a total of 19 participants. From the OTs’ perspectives, our analysis revealed benefits of WSP use and various issues (e.g. some skills seem unrealistic) affecting its uptake in relation to the constructs of the CFIR Intervention Characteristics domain. The results provided guidance for the recommendations of adaptations (e.g. addition of a caregiver assistance score) to enhance implementation of the WSP in pediatric rehabilitation settings and helped to identify the need for the production of new knowledge and knowledge translation (KT) tools.Conclusions: Implementation of the WSP with the adaptations and KT tools proposed could allow pediatric manual wheelchair users to improve their wheelchair skills.
Body Fat Distribution in Trunk and Legs Are Associated With Cardiometabolic Risk Clustering Among Chinese Adolescents Aged 10-18 Years Old
Authors: Yang Qingmei , Ma Ping , Zhang Hao , Cai Rongrong , Dong Yangyang , Ding Wenqing 
Published: October 2020

Abstract
Background: The association between total fat, body fat distribution and cardiometabolic risk factors are highly controversial among adolescents. The aim of the present study was to evaluate the association of body fat distribution with cardiometabolic risk factors clustering among Chinese adolescents.Methods: In this cross-sectional study a total of 1175 adolescents aged 10 to 18 years underwent a comprehensive assessment of cardimetabolic risk factors. Body fat analysis was performed with Bioelectrical impedance analysis. Results: Individuals with the CVRFs ≥1 or CVRFs ≥2 had higher indices of body fat distribution such as body fat mass(BFM) compared to those with normal CVRFs (all P<0.001). The prevalence of CVRFs ≥1, CVRFs ≥2 increased with increasing of the quartile of BFM, TBFM, ABFM, LBFM, PBF, VFL compared to normal subjects. After adjusted for age and sex, the study indicated an linear relationship between TBFM(β=0.693, 95%CI:0.363,1.023), LBFM(β=-1.471, 95%CI:-2.768,-0.175) and CVRFs z-score. Logistic regression models suggested TBFM was associated with CVRFs≥1 and CVRFs≥2 by higher odds. Lower odds of LBFM was associated with CVRFs≥2. Conclusions A significant association between body fat mass of trunk(TBFM), body fat mass of leg(LBFM) and cluster of cardiometabolic risk factors was showed in the study. It suggested to reduce body fat mass of trunk and increase body fat mass of leg were essential for adolescents to prevent cardiovascular risk factors clustering.
Factors Associated With Practice of Chemical Pesticide Use and Acute Poisoning Experienced by Farmers in Chitwan District, Nepal
Authors: KAFLE SIMRIN , Vaidya Abhinav , Pradhan Bandana , Jørs Erik , Onta Sharad 
Published: October 2020

Abstract
Background: Irrational use and unsafe handling of pesticides in agriculture farming is a serious public health concern in developing countries including Nepal. This study was conducted to assess the practice of chemical pesticide use and associated factors along with the acute health symptoms experienced by farmers in Chitwan District, Nepal.Methods: This was a descriptive cross-sectional study using quantitative methods with 790 farmers from Chitwan district, Nepal selected through systematic random sampling. Scores for knowledge, attitude/perception and practice were calculated by aggregating variables where each variable was scored 1 or 0 based on a right or wrong answer. Further, the practice variable was segregated into three groups namely practice during purchasing, practice during mixing and spraying and practice during storage and disposal. Statistical analysis was performed with X2 test, T-test and Multiple Logistic Regression. Confounder controls were made for age, sex and education.Results: Out of the 790 farmers, 663 (84.0%) were found to use exclusively chemical pesticide. Among them, 62% had no idea about its label. Farmers with better knowledge on pesticide handling were 8.3 times more likely to practice safe purchasing (95% CI=5.0-13.8), four times more likely to practice safe mixing and spraying (95% CI=2.5-5.9) and two times more likely to safe storage and disposal (95% CI=1.6-3.6). Similarly, perception/attitude of farmers about chemical pesticide policy and market management was significantly associated with the practice of farmers during purchasing (95% CI=1.1-2.8), mixing and spraying (95% CI=2.0-4.5) and storage and disposal (95% CI=1.1-2.5). Farmers with unsafe practice of pesticide handling were two times more likely to suffer from acute poisoning (AOR = 2.1, 95% CI = 1.3-3.3).Conclusion: There was a high use of pesticide in agriculture farming in Chitwan District. Improving knowledge of farmers on safe handling of pesticides and developing strategies for proper market management would promote the safe handling practices among farmers and reduce the acute pesticide poisoning in Nepal.
New Inside Into Clinical Heterogeneity and Inheritance Diversity of FBLN5-Related Cutis Laxa
Authors: Gharesouran Jalal , Hosseinzadeh Hassan , Ghafouri-Fard Soudeh , Moghadam Yalda Jabbari , Heris Javad Ahmadian , Jafari-Rouhi Amir Hossein , Taheri Mohammad , Rezazadeh Maryam 
Published: October 2020

Abstract
BackgroundFBLN5-related cutis laxa (CL) is a rare disorder that involves elastic ﬁber-enriched tissues and is characterized by lax skin and variable systemic involvement such as pulmonary emphysema, arterial involvement, inguinal hernias, hollow viscus diverticula and pyloric stenosis. This type of CL follows mostly autosomal recessive (AR) and less commonly autosomal dominant patterns of inheritance. ResultsIn this study, we detected a novel homozygous missense variant in exon 6 of FBLN5 gene (c.G544C, p.A182P) by using whole exome sequencing in a consanguineous Iranian family with two affected members. Our twin patients showed some of the clinical manifestation of FBLN5-related CL but they did not present pulmonary complications, gastrointestinal and genitourinary abnormalities. The notable thing about this monozygotic twin sisters is that only one of them showed ventricular septal defect (VSD), suggesting that this type of CL has intrafamilial variability. Co-segregation analysis showed the patients’ parents and relatives were heterozygous for detected variation suggesting AR form of the CL. In silico prediction tools showed that this mutation is pathogenic and 3D modeling of the normal and mutant protein revealed relative structural alteration of fibulin-5 suggesting that the A182P can contribute to the CL phenotype via the combined effect of lack of protein function and partly misfolding-associated toxicity.ConclusionWe underlined the probable roles and functions of the involved domain of fibulin-5 and proposed some possible mechanisms involved in AR form of FBLN5-related CL. However, further functional studies and subsequent clinical and molecular investigations are needed to confirm our findings.
Transpedicular Bi-vertebrae Wedge Osteotomy in Treatment of Post-tubercular Spinal Deformity: A Retrospective Study
Authors: HUANG Yi , HU Wenhao , LI Jing , WANG Tianhao , LIU Huawei , ZHENG Guoquan , ZHANG Xuesong , WANG Yan 
Published: October 2020

Abstract
Background: In the late stage of spinal tuberculosis, the bony destruction and vertebral collapse often leads to significant kyphosis, presenting clinically as a painful gibbus deformity, with increased instability, vertebral body translations and increased risk of neurologic involvement. Spinal osteotomy is thought to be suitable for most patients with severe rigid kyphosis. The aim of this study was to evaluate the efficacy of transpedicular bi-vertebrae osteotomy technique in the patients with Pott’s kyphosis and other post-tubercular spinal deformity.Methods: Between January 2012 and December 2015, 18 patients with post-tubercular spinal deformity underwent the transpedicular bi-vertebrae wedge osteotomy, with a minimum follow up of 27.0 months. Preoperative and postoperative kyphotic angle, sagittal plane parameters (T1-T12 angle for thoracic deformity, T10-L2 angle for thoracolumbar and L1-L5 angle for lumbar deformity) and sagittal vertical axis (SVA) were measured. Oswestry Disability Index (ODI), Visual analog scale (VAS) and modified American Spinal Injury Association grading(ASIA) of preoperative and final follow-up were documented. Results:The average operation time was 455 minutes (range, 200-260 minutes) with a mean intraoperative blood loss of 425 mL (range, 200-700 mL). The kyphotic angles decreased from 80.3° (range, 28.5°-130.8°) preoperatively to 26.1° (range, 7.0°-63.3°) at the final follow-up (P<0.01). The mean VAS score was reduced from preoperative 5.2(range, 2-9) to 0.9(range, 0-2, P<0.01) and the ODI improved from 55.3% (range, 46%-76%) to 6.3% (range, 2%-18%, P<0.01). At final follow-up, there was radiographic evidence of solid fusion at the osteotomy site and fixed segments in all patients. Neurological function improved from ASIA scale D to E in 7 patients, C to D in 3 patients.Conclusions:Our results suggest that transpedicular bi-vertebrae wedge osteotomy is a safe and effective treatment option for post-tubercular spinal deformity. This technique achieves satisfying correction and fusion rates with adequate decompression of neurological elements.
Neodymium-doping Concentration Induced Face-shared to Corner-shared Transition in Strontium Cobaltite
Authors: Huang Haoliang , Chen Zezhi , Xie Liang , Wang Jianlin , Peng Ranran , Fu Zhengping , Lu Yalin 
Published: October 2020

Abstract
The topotactic connection style of oxygen octahedron/tetrahedron in transition metal oxides (TMOs) is an important feature that modulates their corresponding physical properties. Using a simple chemical doping technique, we obtained Sr1-xNdxCoO3-δ with a crystal structure transition from face-shared octahedron to corner-shared octahedron/tetrahedron. The Rietveld analyses of the x-ray diffraction (XRD) patterns show that the crystal structure changes from rhombohedral to cubic and the connection style transforms from face-shared to corner-shared with the increase neodymium (Nd) content. During this process, the ferromagnetic behavior is greatly improved due to the larger amount of the corner-shared cubic SrCoO3-δ phase. The synchrotron radiation x-ray absorption spectroscopies of the Co L-edge and O K-edge show that Nd doping mainly affects the electronic structure of oxygen rather than the valence state of Co. Thereby, the Nd changes the connection style of oxygen octahedron/tetrahedron, which then alters the magnetic interactions.
WGCNA Co-Expression Network Analysis Reveals LncRNA LINC00313 Functions as A ceRNA to Regulate PTEN-Mediated PI3K/AKT Signaling Pathway by Sponging miR-19a-3p in Docetaxel-Resistant Prostate Cancer Cells
Authors: Chen Bin shen , Xu Kai , Zhang Yiming , Xu Peng , Li Chaoming , Liu Jun , Xu Yawen 
Published: October 2020

Abstract
Background: Docetaxel is the preferred chemotherapeutic agent for patients with hormone-refractory PCa. LncRNAs play important roles in the development of docetaxel resistance in patients with PCa. We evaluated the effects of the lncRNA LINC00313 on growth and metastasis in docetaxel-resistant PCa cells, and the molecular mechanisms underlying these effects.Methods: The mRNA dataset (GSE102124 and GSE55945) and lncRNA dataset (GSE115414) were downloaded from the GEO database. The DEGs were identified using the “R package limma”. WGCNA were performed to identify the modules and genes associated with PCa. A PPI network was constructed using the STRING database and visualized using Cytoscape. The Cytohubba plug-in was used to identify the hub genes and cytoNCA plug-in was used to identify the highest linkage hub genes. Based on lncRNA-miRNA pairs and miRNA-mRNA pairs using StarBase and TargetScan databases, a ceRNA network was constructed. Functional analysis was performed via GO, GSEA, and GSVA analysis. Cell proliferation, apoptosis, cell cycle progression, migration, and invasion were evaluated by CCK-8, colony formation, flow cytometry, transwell, and wound healing assays. RT-PCR, western blot, and immunohistochemistry were used to evaluate mRNA and protein expression. The oncogenicity of LINC00313 in docetaxel-resistant PCa cells was determined by tumor transplantation in nude mice. Luciferase reporter and RNA pull-down assays were used to evaluate interactions between LINC00313 and miR-19a-3p as well as between miR-19a-3p and PTEN.Results: LINC00313-miR-19a-3p-PTEN was a core network of ceRNA network. Cox regression analysis identified LINC00313 as an independent prognostic variable for OS in patients with PCa. LINC00313 overexpression in docetaxel-resistant PCa cells suppressed proliferation, invasion, and migration, and induced apoptosis and cell cycle arrested at the G1 phase. These changes were related to changes in cyclinD1, p27, Ki67, Bax, Bcl-2, caspase-3/9, E-cadherin, N-cadherin, Vimentin, and MMP-2 expression. Animal experiments showed that LINC00313 silencing promoted tumor formation. Co-transfection with LINC00313‐WT and miR-19a-3p mimic reduced luciferase activity, and co-transfection with PTEN‐WT and LINC00313 increased luciferase activity. The effects of LINC00313, including increased PTEN, decreased PIP3 expression, and the inhibition of proliferation and metastasis in docetaxel-resistant PC cells, were abolished by miR-19a-3p mimic.Conclusion: LINC00313 overexpression suppressed the proliferation and metastasis of docetaxel-resistant PC cells by competitively binding to miR-19a-3p to regulate PTEN expression and inhibit the activation of the PI3K/Akt signaling pathway.