bluuebunny/crossref_metadata_2025_split huggingface dataset:
Engineering of The Bacillus Subtilis PhoD Signal Peptide To Direct High-Level, Tat-Specific Export of A Single-Chain Antibody Fragment To The Periplasm in Eschericha Coli
Authors: Jawara Chillel , Richards Kirsty L , Peswani Amber R , Walker Kelly L , Nascimento Lara , Robinson Colin 
Published: August 2020

Abstract
Background: Numerous high-value proteins have been produced in E. coli, and a favoured strategy is to export the protein of interest to the periplasm by means of an N-terminal signal peptide. While the Sec pathway has been extensively used for this purpose, the Tat pathway has potential because it transports fully-folded heterologous proteins. Most studies on the Tat pathway have used the E. coli TorA signal peptide to direct export, because it is highly Tat-specific, unlike many Tat signal peptides which can also function as Sec signal peptides. However, the TorA signal peptide is prone to degradation in the cytoplasm, leading to reduced export rates in some cases. Here, we have tested a range of alternative signal peptides for their ability to direct Tat-dependent export of a single-chain antibody fragment (scFv). Results: We show that the signal peptides of E. coli AmiC, MdoD and YcbK direct efficient export of the scFv by both the Tat and Sec pathways, which may be a disadvantage when Tat-specific export is required. The same applies to the Tat signal peptide of Bacillus subtilis PhoD, which likewise directs efficient export by Sec. We engineered the PhoD signal peptide by introduction of a Lys or Asn residue in the C-terminal domain of the signal peptide, and we show that this substitution renders the signal peptide Tat-specific. These signal peptides, designated PhoDk and PhoDn, direct efficient export of scFv in shake flask and fed-batch fermentation studies, reaching export levels that are well above those obtained with the TorA signal peptide. Culturing in ambr250 bioreactors was used to fine-tune the growth conditions, and the net result was export of the scFv by the Tat pathway at levels of approximately 1g protein/L culture. Conclusions: The new PhoDn and PhoDk signal peptides have significant potential for the export of heterologous proteins by the Tat system.
Circulating IgA1 Complex Induces Endothelial Injury through Mesangial Cell Activation in IgA Nephropathy
Authors: Huang Meichun , Lu Ying , Zhao Liping , Ding Huideng , Liu Jun , Yan Xiaoqian , Zhu Li , Shi Sufang 
Published: August 2020

Abstract
Background: Renal arteriolar microangiopathic (MA) lesions were common in IgA nephropathy (IgAN) and their presence was an independent risk factor for kidney failure. Endothelial injury is regarded as the most important cause of MA lesions. The deposition of circulating IgA1 complex (cIgA1) in the glomerular mesangium has been regarded as the most important mechanism to induce the injury of podocyte and tubular epithelium. Whether cIgA1 can induce vascular endothelial injury is not clear.Methods: Blood of 59 IgAN patients and 19 healthy controls was collected, pending the measurement of Willebrand factor (vWF) and soluble vascular cell adhesion molecule 1 (sVCAM1) and Heparin-binding EGF-like growth factor (HB-EGF) by ELISA. cIgA1 was isolated from 12 primary IgAN patients who had not been treated with steroids or immunosuppressants and 10 healthy controls. Then, IgA-HMC medium (IgA-HMCM) was prepared by collecting the cell culture supernatants of growth-arrested HMCs grown in medium containing cIgA1. Human umbilical vein endothelial cells (HUVECs) were cultured with the IgA-HMCM in the presence or absence of Panax notoginseng saponins (PNS), which are anti-inflammatory bioactive components of the Chinese medicinal herb Panax notoginseng. The protein levels of interleukin-6 (IL-6) and chemokine (C-X-C motif) ligand 1 (CXCL1) in IgA-HMCM and vWF, sVCAM1 and HB-EGF in HUVEC medium were determined by ELISA. Results: IgAN patients had higher levels of vWF, sVCAM-1 and HB-EGF than that in healthy controls. cIgA1 from IgAN patients (IgAN-cIgA1) induced significantly higher expression levels of IL-6 and CXCL1 in HMCM than cIgA1 from healthy controls. vWF, sVCAM1 and HB-EGF levels induced by conditioned IgAN-cIgA1-treated HMCM (IgAN-HMCM) were significantly higher than the levels induced by conditioned cIgA1-treated HMCM from healthy controls (HC-HMCM). After HUVECs were cultured in PNS and IgAN-HMCM, the levels of vWF, sVCAM1 and HB-EGF were significantly lower in the culture supernatants of cells cultured in PNS and IgAN-HMCM than in that of cells cultured in IgAN-HMCM alone. Conclusion: cIgA1 could activate mesangial cells and, in turn, induce the injury of endothelial cells by inflammation factors in vitro.
A Collagen Membrane Influences Bone Turnover Marker in Vivo After Bone Augmentation With Xenogenic Bone
Authors: Staedt Henning , Dau Michael , Schiegnitz Eik , Thiem Daniel G.E. , Tagadiuc Olga , Palarie Victor , Ottl Peter , Al-Nawas Bilal , Kammerer Peer W 
Published: August 2020

Abstract
Background:The aim was to compare early biochemical and histological osseous healing of chronic mandibular defects regenerated with bovine bone substitute with and without collagen membranein vivo. Methods:Eight weeks after formation of a lateral defect in the mandible of 40 rabbits, bovine bone substitute with (“+”;n=20) and without (“-“;n=20) collagen membrane was applied. Blood and bone was collected 24, 72 hours, 7, 14 and 21 days after surgery. Total acid phosphatase, bone acid phosphatase, total alkaline phosphatase and bone alkaline phosphatase activities were compared between groups. Formation of new bone was quantified histologically for all time points.Results:24 hours after surgery, bone alkaline phosphatase was significantly elevated in “+” group when compared to “-“ (p=0.012). After 72 hours, all bone turnover markers except for total acid phosphatase (p=0.078) where significantly elevated in “+” (all p<0.05). 14 days after surgery, the significant highest values for all bone turnover markers were detected in “-“ (all p<0.05). A significant difference in favor of group “-“ could also be detected after 3 weeks in terms of both acid phosphatases (p<0.05). In histology, no significant differences could be detected.Conclusion: Bone regeneration with bovine bone substitute material and collagen membrane shows a significantly earlier bone remodeling activity but does not seem to influence formation of new bone in histological samples.
MiR-124-3p/ZC3H15 Regulates Gastric Cancer Progression by Blocking FBXW7 Mediated Degradation of c-Myc
Authors: Hou Jianbing , Liu Yudong , Yan Du , Huang Pan , Wang Zhongze , Gu Hongyu , Lan Chao , Geng Shengjun , Cui Hongjuan 
Published: August 2020

Abstract
BACKGROUND: Zinc finger CCCH-type containing 15 (ZC3H15), a highly conserved eukaryotic protein, was involved in tumorigenesis and may be a potential biomarker in hepatocellular carcinoma (HCC) and acute myeloid leukemia (AML). However, the biological role of ZC3H15 in gastric cancer (GC) is unclear.METHODS: The potential correlation between ZC3H15 expression and GC prognosis was assessed based on the patient data analysis. The biological role of ZC3H15 in regulating cell proliferation and metastasis was evaluated in vitro and in vivo. In addition, the potential mechanism of ZC3H15 was investigated. RESULTS: we found that ZC3H15 expression was positively correlated with GC progression, including cell growth, metastasis and cancerogenesis. Through further investigations, we found that ZC3H15 could modulate c-Myc protein stability via suppressing the transcription of FBXW7, which was mainly responsible for c-Myc degradation. In addition, we revealed that miR-124-3p, a tumor suppressor of GC, was negatively associated with ZC3H15. We revealed that miR-124-3p was a critical upstream modulator of ZC3H15 in GC.CONCLUSIONS: Taken together, our studies unearth the important roles of ZC3H15 in GC development and suggest that miR-124-3p/ZC3H15/c-Myc axis may be a potential target for the treatment of GC.
Environmental and Nutritional Challenges With Down's Syndrome
Authors: Aljerf Loai , Aljurf Mazen 
Published: August 2020

Abstract
Background

Down's syndrome patients are suffering from such gastrointestinal and metabolic complications which affect their prolongation for survival and this could be attributed to the malnutrition system. To reduce the risk factors for mortality, the paper has focused on the assessment of the socioeconomic, clinical, physical, biophysiological, and biochemical characteristics of them which can be affected by the type of nutrition system, toxicity, and ecological footprint.
Methods

Patients were males with trisomy 21 diagnosed by karyotype test and assessed by clinical examinations. The clinical observations, medical interventions, and oral diseases associated with DS have been defined and oral treatment is explored. Samples collected from different biofluids. The physicochemical analyses of the biomatrix samples were performed and these properties had compared to findings of healthy males and age-matched controls. In specific, trace elements which could be originated from environmental resources were assessed in saliva, blood, urine, and hair.
Results

Duraphat application was proved effective for their oral treatment and saliva was the optimum biomarker for detecting malnutrition. The patients were hypersensitive to Cu while the Mn content in blood and hair was considered an expression to the degree of epileptic condition and chronic seizure development. The ecological footprint was 5.6 gha and carbon footprint was recognised in food poverty habits. These can be reduced by eating more plant-based proteins and fibre-rich foods with low saturated fats and sodium.
Conclusion

The current findings provide an up-to-date reference for expected developmental outcomes in children with DS in terms of biophysicochemistry. The genetically sensitive intervention is affected by heredity factor and sensitivity to toxics. Down's syndrome is encouraged to live green-hipster life. Besides, doctors are recommended to order the physicochemical analyses for early detection of this special intervention.
Membrane and lipid metabolism plays an important role in desiccation resistance in the yeast Saccharomyces cerevisiae
Authors: Ren Qun , Brenner Rebecca , Boothby Thomas C. , Zhang Zhaojie 
Published: August 2020

Abstract
Background: Anhydrobiotes, such as the yeast Saccharomyces cerevisiae , are capable of surviving almost total loss of water. Desiccation tolerance requires an interplay of multiple events, including preserving the protein function and membrane integrity, preventing and mitigating oxidative stress, maintaining certain level of energy required for cellular activities in the desiccated state. Many of these crucial processes can be controlled and modulated at the level of organelle morphology and dynamics. However, little is understood about what organelle perturbations manifest in desiccation-sensitive cells as a consequence of drying or how this differs from organelle biology in desiccation-tolerant organisms undergoing anhydrobiosis. Results: In this study, electron and optical microscopy was used to examine the dynamic changes of yeast cells during the desiccation process. Dramatic structural changes were observed during the desiccation process, including the diminishing of vacuoles, and increase and then decrease of lipid droplets as well as a decrease in mitochondria and mitochondrial cristae and an increase of ER membrane, which is likely caused by ER stress and unfolded protein response. The survival rate was significantly decreased in mutants that are defective in lipid droplet biosynthesis, or cells treated with cerulenin, an inhibitor of fatty acid synthesis. Conclusion: Our study suggests that the metabolism of lipid droplets and membrane may play an important role in yeast desiccation tolerance by providing cells with energy and possibly metabolic water. Additionally, the decrease in mitochondria and cristae coupled with an initial increase and then drop in lipid droplets number is indicative of a cellular response to reduce the production of reactive oxygen species.
Producción de forrajes de avena y trigo bajo sistemas hidropónico y convencional
Authors: Morales Sinchire Dayana Beatriz , Jiménez Álvarez Leticia Salomé , Burneo Valdivieso Juan Ignacio , Capa Mora Edwin Daniel 
Published: August 2020

En la Región Sur del Ecuador se comparó la producción de avena (Avena sativa L.) y trigo (Triticum vulgare L.) obtenida a partir de un sistema de forraje verde hidropónico (FVH), frente al sistema de producción convencional o a campo abierto (CA). El experimento para cada sistema y cultivo se estableció bajo un diseño de bloques al azar, instalando cuatro tratamientos (dos en FVH y dos a CA), con tres repeticiones cada uno. Se evaluó la altura de la planta (AP) de cada cultivo para cada sistema productivo, mientras que para cada sistema y cultivo se determinó la producción de biomasa, la proteína cruda (PC) (p < 0,05) y el cociente de la relación beneficio-costo (B/C). La mayor altura se obtuvo en trigo FVH con 17,67 cm y en avena CA con 82,78 cm. En relación con la producción de biomasa, el trigo FVH alcanzó 23,57 kg/m2 de materia verde (MV) o 3,10 kg/m2 de materia seca (MS); el resto de tratamientos estuvo por debajo de 13 kg/m2 de MV (1,39 kg/m2 de MS); el porcentaje de proteína en el trigo CA fue mayor con 19,90 %, seguido de avena CA, mientras que el trigo y la avena FVH tuvieron valores de alrededor del 10 %; en beneficio-costo, el trigo FVH obtuvo un puntaje mayor de 1,46 frente a la avena FVH (0,67), el trigo CA (0,26) y la avena CA (1,00). Por esta razón, la complementariedad de los dos sistemas de producción, especialmente en el cultivo de trigo, podría ser una opción para la alimentación animal.
Tingkat Tutur Pengisi Fungsi Subjek Bahasa Korea dan Bahasa Jawa
Authors: Hanani Iva , Nugroho Suray Agung 
Published: August 2020

The aim of this study is to explain Korean and Javanese subject honorific and identify its similarities and differences. To accomplish it, this research was conducted using literature review method. Data related to the Javanese language were taken from two Javanese drama scripts, namely Mak Ana Asu Mlebu Ngomah and Bantul Sangsaya Pinunjul, and Javanese drama titled Sri Ngilang by George Quinn. Korean language data was taken from television drama, Misaeng and Fight for My Way. Based on the analysis that has been carried out, despite the difference of the honorific system of Korean dan Javanese, Javanese has subject honorific as described in the Korean honorific system. Both languages use words that have honorific meaning to honor the subject and use words that are usually used for the animal to dishonor the subject of the sentence. The difference between both languages on subject honorific is Korean subject honorific is mainly realized grammatically, whereas in Javanese is realized lexically. In addition, there are rules from the Korean government regarding the use of one type of subject honorific, abjonbeob, whereas in Javanese there are no rules related to the use of speech level.
MODEL EMPIRIS ONLINE TRUST DAN REPEAT PURCHASE PELANGGAN TERHADAP SITUS E-COMMERCE
Authors: Hafni Roswita , Hasibuan Jasman Saripuddin , Muslih Muslih , Yusnandar Willy 
Published: August 2020

Penelitian ini bertujuan untuk mengetahui dan menganalisis model empiris online trust dan repeat purchase pelanggan Terhadap Situs E-Commerce (Studi Kasus pada Pelanggan E-Commerce di Kota Medan). Penelitian ini menggunakan pendekatan penelitian eksplanatori. Skala pengukuran variabel menggunakan Skala Ordinal dengan teknik pengukuran variable menggunakan skala Likert. Populasi dalam penelitian ini adalah masyarakat kota Medan. Metode pengambilan sampel dilakukan dengan metode Purposive sampling (judgmental) dan snowball sampling. Kriteria pemilihan sampel dalam penelitian ini adalah masyarakat kota Medan yang pernah berkunjung dan melakukan transaksi di e-commerce yang ada di Indonesia lebih dari 3 kali. Teknik pengumpulan data yang digunakan yaitu wawancara, kuesioner (angket) dan studi pustaka. Metode analisis data yang digunakan adalah Model Persamaan Struktural (Structural Equation Model). Hasil penelitian menunjukkan bahwa Web Quality, Perceived Risk, dan Online Trust berpengaruh signifikan terhadap Repeat Purchase, begitu juga Web Quality dan Perceived Risk berpengaruh signifikan terhadap online trust. Web Quality berpengaruh terhadap Repeat Puchase melalui variabel Online Trust, dan Perceived Risk berpengaruh terhadap Repeat Purchase melalui variabel Online Trust.
The Role of Islamic Education Teachers Competency in Improving the Quality of Education
Authors: Zuhaeriah Zuhaeriah , Ali Moh. , Yusra Yusra 
Published: August 2020

This study discusses the urgency of the competency of teachers on the subjects of Islamic religious education. This study used descriptive qualitative method and gather data through observation, interview, and documentation. Data analysis was carried out through reduction, data presentation, and data verification that ended with checking the validity of the data. The results of this study shows that competence of teachers in junior high school is adequate. The role of teachers in improving the quality of teaching, positions which directly determine the success, considering the teacher as a figure who is directly involved in learning in the classroom. All of these roles requires more concrete effort, and directly touch to the needs of students in order to get better the quality of learning. The efforts made by the Islamic religious education teachers in improving the quality of education including the provision of guidance to students, advice, supervision of students, providing motivation and rewards, using varied teaching methods, and personal approach to students. Supporting factors include the existence of good cooperation from principals, teachers, parents, staff, students and the local community, the infrastructure, the competent teachers, and safe and orderly environment. Inhibiting factors include the lack of instructional media; in this case the writer mean is computer and LCD, the lack of participation and support fromstudents’ parent. As for efforts to deal with is as follows: add computer and LCD and establish good coordination with parents.
Editorial Preface
Authors: Nurdin Nurdin 
Published: August 2020

International Journal of Contemporary Islamic Education volume 2 issue 1 offers six articles covering topics of Islamic education studies. Various issues relating to Islamic education interestingly presented to contribute to the body of knowledge and practices. Academia and practitioners in Islamic education studies may gain insight from reading the articles.

The first article is titled “Active Learning Strategies Implementation in Arabic Teaching at Senior High School” written by Asmawati Asmawati, Asy’ari Asy’ari, and Malkan Malkan from Postgraduate studies Institut Agama Islam Negeri Palu. This paper discusses the implementation of active learning strategy in Arabic teaching at a senior high school in Palu city. The second article is titled “ Teachers’ Strategy in Improving Students’ Learning Achievement of Al-Qur’an and Hadits at Madrasah Tsanawiyah” written by Mardiah Mardiah, Askar Askar, and Rustina Rustina. The paper discussed the strategies of teachers of the Qur'an and hadith subjects in improving the learning achievement of students in Madrasah Tsanawiyah (MTs) Al-Khairaat Biromaru. The third article is titled “Contribution of Islamic Education Values in Marriage Tradition of Banggai Ethnic, Indonesia” written by Oyan D. Taufik, Sagaf S. Pettalongi, and Sidik Sidik. The paper discusses the contribution of Islamic education values in marriage according to the customs of the tribe in district of Banggai Bulagi. The fourth paper is tittled “The Role of Environment in Improving Learning Quality: A Case Study of Al-Istiqamah Modern Islamic Boarding School” written by Rara Pratiwi, Kamaruddin Kamaruddin, and Adawiyah Adawiyah. The paper discusses the role of learning environment in improving the quality of learning students in modern boarding school Al-Istiqamah Ngatabaru. The fifth article is titled “Effectiveness the Use of Audio Visual Media in Teaching Islamic Religious Education” written by Winarto Winarto, Ahmad Syahid, and Fatimah Saguni. The paper discussed the effectiveness of using audio visual as media in improving learning achievement of a Islamic Junior high school. The last paper is title “The Role of Islamic Education Teachers Competency in Improving the Quality of Education” written by Zuhaeriah Zuhaeriah, Moh. Ali, and Yusra Yusra. The paper discusses the urgency of the competency of teachers on the subjects of Islamic religious education.

I hope the articles presented in this issue add further empirical evidence to the growing body of research that examines various fields from Islam perspectives. The articles could trigger other research to study other field of study with Islam perspectives Islamic institutions in Indonesia.

Nurdin Nurdin
Editor-in-Chief
IJCIED: International Journal of Contemporary Islamic Education
Volume II, Issue 1
Sacubitril/valsartan use in a real-world population of patients with heart failure and reduced ejection fraction
Authors: Mapelli Massimo , Salvioni Elisabetta , de Martino Fabiana , Mattavelli Irene , Bonomi Alice , Sassi Valentina , Gugliandolo Paola , Vignati Carlo , Magini Alessandra , Rovai Sara , Paolillo Stefania , Agostoni Piergiuseppe 
Published: August 2020

Aims
Practice guidelines recommend sacubitril/valsartan for heart failure with reduced ejection fraction. The aim of our study was to describe the use of sacubitril/valsartan in real-world clinical practice to help identify patients best able to tolerate titration to higher doses.

Methods
We retrospectively analyzed clinical data for 201 patients with heart failure with reduced ejection fraction prescribed sacubitril/valsartan at our heart failure clinic (Centro Cardiologico Monzino) between September 2016/December 2018. Patients had a mean age of 67.2 years, mean left ventricular ejection fraction of 30.1%, New York Heart Association class II (65%), class III (35%), and poor cardiopulmonary exercise capacity. Median 2-year risk of death/urgent cardiac transplantation was 8.9% [Metabolic Exercise Cardiac Kidney Index (MECKI) score].

Results
After a median follow-up of 230 (interquartile interval: 105–366) days, 57 patients achieved higher-dose sacubitril/valsartan, 103 tolerated medium/low doses, nine died, and 20 interrupted treatment. The highest dose of sacubitril/valsartan was reached by younger patients with better hemoglobin (Hb) levels, renal function, and blood pressure (BP). Patients continuing on sacubitril/valsartan had significantly higher serum Hb and sodium, better BP, and lower MECKI scores than patients who discontinued treatment or died during follow-up. Our patients were older and frailer than those in the pivotal PARADIGM-HF trial.

Conclusion
In our experience, more than one-third of the patients were able to tolerate the higher dose of sacubitril/valsartan, and these patients were younger, had higher Hb, and better BP and renal function. MECKI score stratification was useful to discriminate patients who continued treatment from those who did not. Future prospective studies should test if these clinical variables can guide the up-titration of sacubitril/valsartan.