bluuebunny/crossref_metadata_2025_split huggingface dataset:
Production of Basil (Ocimum Basilicum L.) Under Different Soilless Cultures
Authors: Khater El-Sayed , Bahnasawy Adel , Abass Wael , Morsy Osama , El-Ghobashy Hossam , Shaban Yousry , Egela Mohsen 
Published: December 2020

Abstract
Soilless cultivation systems that provide plant management in soilless conditions in which the supply of water and of minerals is carried out by nutrient solution, with or without a growing medium. The main aim of this paper was to investigate the possibility of growing basil under three soilless systems (aeroponic, hydroponic and peatmoss slab systems). A model was developed to predict the nutrients consumption by basil plants. Shoot and root height, fresh and dry mass of whole plant, nutrients uptake, and oil content were studied during the growth period (after 4 and 7 weeks from transplanting). The results indicated that the shoot lengths of basil plants were 62.00 ± 2.65, 57.83 ± 7.42 and 48.77 ± 2.89 cm after 4 weeks and 71.67 ± 2.89, 65.67 ± 1.15 and 62.33 ± 2.31cm after 7 weeks from transplanting for aeroponic, hydroponic and peatmoss slabs, respectively. The highest value of root height of basil plants was 37.67 ± 6.66 cm for aeroponic system. The dry mass of shoot of basil plants ranged from 28.48 ± 0.91 to 44.77 ± 0.97 and 72.98 ± 0.83 to 117.93 ± 1.40 g plant-1 after 4 and 7weeks from transplanting, respectively. The highest values of the N, P, K, Ca and Mg uptakes were 262.50 ± 6.84, 74.34 ± 2.90, 195.13 ± 4.09, 132.41 ± 1.54 and 41.81 ± 0.83 mg plant-1 and 753.99 ± 5.65, 224.88 ± 3.05, 449.75 ± 4.59, 529.12 ± 6.63 and 112.44 ± 1.67 mg plant-1 after 4 and 7 weeks from transplanting, respectively. The basil oil content ranged from 1.129 ± 0.020 to 2.520 ± 0.021 and 2.664 ± 0.291 to 6.318 ± 0.375 g plant-1 after 4 and 7weeks from transplanting, respectively at the same pervious order. The production costs of basil plant were 2.93, 5.27 and 6.24 EGP kg-1 of plant. The model results were in a reasonable agreement with the experimental ones.
LncRNA HITT Overexpression in Gastric Cancer Promotes Cell Apoptosis by Suppressing the Maturation of&nbsp;miR-602
Authors: Chen Yun , Ouyang Canhui , Liao Lingyun , Zhou Yun , Meng Fan , Liu Yao , Ye Jing 
Published: December 2020

Abstract
Background: HITT inhibits colon cancer. This study explored its role in gastric cancer (GC).Methods: HITT, mature miR-602 and premature miR-602 expression in paired GC and normal tissues (62 patients) was studied by RT-qPCR. RNA pull-down assay was performed to analyze the direct interaction between HITT and mature miR-602. The subcellular location of HITT was analyzed by nuclear fractionation assay. The role of HITT in regulating miR-602 maturation was analyzed by overexpression assay. Cell apoptosis was analyzed by flow cytometry.Result: Our assays illustrated that HITT was highly expressed in GC and mature miR-602 was lowly expressed in GC. No alteration in premature miR-602 in GC was observed. HITT was located to both nucleus and cytoplasm, and it can directly interact with miR-602. HITT overexpression in GC cells increased the expression of mature miR-602 but not premature miR-602. HITT overexpression increased GC cell apoptosis and suppressed the role of miR-602 in inhibiting GC cell apoptosis. Conclusion: Therefore, HITT may promote GC cell apoptosis by suppressing the maturation of miR-602.
Medical and Economics Benefits of Tele-expertise in Dermatology: Case study in eight Health Centers in Mali.
Authors: Bagayoko Cheick Oumar , Cissé Mahmoud , Aka Joseph , Dicko Adama , Anne Abdrahamane , Traoré Dramane , Cissé Lamissa , Niang Mahamoudane , Karabinta Yamoussa , Gassama Mamadou , Sidibé Modibo , Faye Ousmane 
Published: December 2020

Abstract
Background: Mali, like many Sub-Saharan African countries, is experiencing an acute crisis with respect to the shortage of qualified health professionals. This crisis is even more acute when it comes to specialized medical fields such as dermatology. To address this shortage, a tele-dermatology project has been launched in Mali since 2015 in order to provide access to specialized care to the most remote populations.Objective: The aim of our study is to assess the medico-economic benefits of the pilot phase of this project.Methods: We conducted a retrospective cross-sectional study of all requests for expert advice sent to dermatology experts through the "Bogou" tele-expertise platform.The sample consisted of 52 patients at eight remote sites and ten health professionals, including 4 specialists.The economic study was done using a parameter of cost analysis and the professional evaluation of healthcare providers. It compared consultation and transportation fees (on-site and off-site), and used a questionnaire assessing items on a 5-level Lickert scale and open-ended questions to evaluate the satisfaction of health professionals. Data analysis was performed by SPSS v25.Results: During the period, 374 requests for tele-expertise were made for the benefit of 52 patients. 89.3% of requests were answered by specialists, with an average response time of 46 hours 59 minutes and extremes from 7 minutes to 415 hours 4 minutes. Eczema was the most common medical condition diagnosed. 98% of patients had never completed a dermatological consultation because of a lack of means to travel to the only specialized center in the country. Consequently, they were all very satisfied or satisfied with the tele-expertise service. The 52 participating patients in project have realized a great financial benefit. Together they saved an average of 5,824,500 XOF (9429 euros).All the health professionals surveyed also expressed satisfaction with the application. They confirmed that the project has enabled them to strengthen their skills in the management of dermatological pathologies.Conclusion: Based on the results of our study, we can say that tele-dermatology allows access to specialized care and a reduction in the costs of care for patients in remote areas.
Staffing and Patient-related Factors Affecting in-patient Fall-related Injury in a Psychiatric Hospital: A 5-year Retrospective Matched Case-control Study
Authors: Seeherunwong Acharaporn , Thunyadee Chanya , Vanichakit Wipa , thanabodeethumacharee Pavinee 
Published: December 2020

Abstract
Background: Incidence of fall-related injury of psychiatric in-patients was more frequent and the severity greater than in general units. Also, the risk factors were different and more complicated. The aim of this study was to investigate the staffing factors and patient-related factors that increase the likelihood of fall-related injury among psychiatric in-patients. Methods: A five-year retrospective matched case-control study was used to select cases of in-patient fall which were reported to the hospital risk management system. The total sample consisted of 240 patients. Conditional logistic regression was used to analyze the data. Results: Of the eighty fall-related injuries, 86.3% resulted in temporary harm and required intervention. The multivariate model showed that the three strongest predictors were having an acute psychotic condition (adjusted matched odds ratio (aOR)=5.40; 95%CI 4.22-6.90; p< 0.001), proportion of nurse staffing equal to nurse assistants (aOR=5.52; 95%CI 2.64-11.55; p<0.001), and taking atypical antipsychotic drug (aOR=3.92; 95%CI 3.22-4.76; p<0.001). In addition, the following factors all increased the risk of fall-related injury significantly: using more than four drugs, having a medical illness, having comorbid psychiatric disorder, taking lithium, anti-Parkinson, benzodiazepine, and anti-convulsant drugs. Regarding staffing factors, patient numbers in the unit of 25-50, and ≥ 51, also significantly increased risk for fall than having patient numbers of 25 or fewer. Nurse to patient ratios of 1:16-30, and 1: 31-45 significantly increased the fall risk compared to ratios of 1: 1-15. Conclusions: The risk factors found are expected to be of use for assessing fall risk and managing staff workload in psychiatric units.
Supervised Binary Classification Methods for Strawberry Ripeness Discrimination from Bioimpedance Data
Authors: Ibba Pietro , Tronstad Christian , Moscetti Roberto , Mimmo Tanja , Cantarella Giuseppe , Petti Luisa , Martinsen Ørjan , Cesco Stefano , Lugli Paolo 
Published: December 2020

Abstract
Strawberry is one of the most popular fruits in the market. To meet the demanding consumer and market quality standards, there is a strong need for an on-site, accurate and reliable grading system during the whole harvesting process. In this work, a total of 923 strawberry fruit were measured directly on-plant at different ripening stages by means of bioimpedance data, collected at frequencies between 20Hz and 300kHz. The fruit batch was then splitted in 2 classes (i.e. ripe and unripe) based on surface color data. Starting from these data, six of the most commonly used supervised machine learning classification techniques, i.e. Logistic Regression (LR), Binary Decision Trees (DT), Naive Bayes Classifiers (NBC), K-NearestNeighbors (KNN), Support Vector Machine (SVM) and Multi-Layer Perceptron Networks (MLP), were employed, optimized, tested and compared in view of their performance in predicting the strawberry fruit ripening stage. Such models were trained to develop a complete feature selection and optimization pipeline, not yet available for bioimpedance data analysis of fruit. The classification results highlighted that, among all the tested methods, MLP networks had the best performances on the test set, with 0.72, 0.82 and 0.73 for the F1, F0.5 and F2-score, respectively, and improved the training results, showing good generalization capability, adapting well to new, previously unseen data. Consequently, the MLP models, trained with bioimpedance data, are a promising alternative for real–time estimation of strawberry ripeness directly on–field, which could be a potential application technique for evaluating the harvesting time management for farmers and producers.
The Effect of Rapid Maxillary Expansion on the Upper Airway’s Aerodynamic Characteristics
Authors: Feng Xin , Chen Yicheng , Halme Kristina , Cai Weihua , Shi Xie-Qi 
Published: December 2020

Abstract
Background: The effect of rapid maxillary expansion (RME) on the upper airway (UA) has been studied earlier but without a consistent conclusion. This study aims to evaluate the outcome of RME on the upper airway function in terms of airflow resistance by applying a computational fluid dynamics (CFD) simulation.Methods: This retrospective cohort study consists of seventeen cases with two consecutive CBCT scans obtained before (T0) and after (T1) RME. Patients were divided into two groups with respect to patency of the nasopharyngeal airway as expressed in the adenoidal nasopharyngeal ratio (AN): group 1 was comprised of patients with an AN ratio < 0.6 and group 2 encompassing those with an AN ratio ≥ 0.6. CFD simulation at inspiration and expiration were performed based on the three - dimensional (3D) models of the UA segmented from the CBCT images. The aerodynamic characteristics in terms of pressure drop, midsagittal maximum velocity, and maximum wall shear stress were compared by independent samples t-test between the two groups at T0 and T1. Results: At T0, the pressure drop at expiration was significantly higher in group 2 compared to group 1, whereas the difference between the two groups was not significant at T1. The midsagittal maximum velocity of the two groups decreased to some extent at expiration, but without significant difference. The midsagittal maximum velocity of group 2 was at inspiration significantly higher than that of group 1 at T0 and T1. Conclusion: RME had a positive effect on UA airflow resistance in patients with an AN ratio ≥ 0.6. The findings provide positive evidence of RME in airway function and should thus be recommended to patients with both a narrow maxilla and enlarged adenoid.
Synergistic effects of D-arginine, D-methionine and D-histidine on the inhibition and eradication of Porphyromonas gingivalis biofilm
Authors: Zhang Zhenyang , Li Baosheng , Cai Qing , Qiao Shuwei , Wang Dan , Wang Heling , Zhang Huiyan , Yang Yalan , Meng Weiyan 
Published: December 2020

Abstract
Porphyromonas gingivalis (P. gingivalis) biofilm is involved in peri-implantitis and periodontitis. Whether a mixture of D-AAs has synergistic effects on P. gingivalis biofilm remains unknown. The aim of this study was to investigate the effects of multiple D-AAs on P. gingivalis biofilm. D-arginine (R), D-methionine (M) and D-histidine (H) were used. The bacterial growth activity and minimum inhibitory concentrations (MICs) were determined. The effects of D-AAs mixture on biofilm biomass, extracellular polymeric substances (EPS), biofilm morphology, cytotoxicity, biofilm structure and bacterial membrane integrity were determined. D-AAs mixture delayed the proliferation of P. gingivalis below MICs. Mixtures of D-AAs displayed definite effects on the inhibition and disassembly of biofilm than single D-AAs. The EPS content increased with D-AAs concentrations. D-AAs mixture damaged the integrity of biofilm, while RMH showed the best effects. 8 mM RH and 4 mM RMH had no cytotoxicity. 4 mM RMH decreased biofilm thickness and altered bacteria membranes structure. Hence, the combined mixture of 4 mM RMH presents the best synergistic effects on the inhibition and eradication of P. gingivalis biofilm. Our study provides new insights of application of D-AAs for the treatment of peri-implantitis and periodontitis.
Anti-inflammatory diet consumption reduced fatty liver indices: Finding from a large cohort study in the Kurdish population
Authors: Darbandi Mitra , Hamzeh Behrooz , Ayenepour Azad , Rezaeian Shahab , Najafi Farid , Shakiba Ebrahim , Pasdar Yahya 
Published: December 2020

Abstract
Background

The aim of this study was the assess association between of dietary inflammatory index (DII) and non-invasive markers of liver status in adults.
Methods

This cross-sectional study was performed on 8,520 individuals aged 35–65 years, recruited in Ravansar Non-Communicable Diseases (RaNCD) cohort study, the west of Iran. DII score was calculated based on participants’ dietary intakes obtained from Food Frequency Questionnaire (FFQ). Fatty Liver Index (FLI) score was calculated by anthropometric measurements and some non-invasive markers of liver status. Linear regression models were used to estimate the associations and adjust for possible confounding factors.
Results

The mean of age was 47.24 ± 8.31 years and 4275 (%50.18) were male. A greater DII score was significantly associated with higher energy intake, BMI (body mass index), BFM (body fat mass), blood pressure and FLI (P < 0.001). Participants with the highest DII score had a significantly higher consumption saturated fat, trans fat and red meat than those in the lowest quartile (P < 0.001). With one point increment in the physical activity, on average the risk of having FLI decreased by 5 (95% CI, -0.484 to -0.519). After adjustment for age and sex, participants in the highest quartile of DII score had a greater risk of FLI (β: 0.742, 95% CI: 0.254, 0.601).
Conclusion

The more pro-inflammatory diet in participants was associated with higher FLI; DII score was positively associated with non-invasive liver markers (ALT, AST and GGT). FLI had positive association with BMI, WC, BFM and energy intake. Thus having an anti-inflammatory diet can help balance liver enzymes, reduce obesity and fatty liver.
Il-1Β Primed Mesenchymal Stromal Cells Moderate Hemorrhagic Shock Induced Organ Injuries
Authors: Aussel Clotilde , Baudry Nathalie , Grosbot Marion , Caron Cécile , Vicaut Eric , Banzet Sébastien , Peltzer Juliette 
Published: December 2020

Abstract
BackgroundOrgan damages following hemorrhagic shock (HS) have been partly attributed to an immunological dysfunction. The current challenge in the management of HS patients is to prevent organ injury-induced morbidity and mortality which currently has not etiological treatment available. Mesenchymal stromal cells (MSC) are used in clinical cell therapy for immunomodulation and tissue repair. In vitro priming is often used to improve the immunomodulation efficiency of MSC before administration.ObjectiveAssess the effect of naive MSC (MSCn) or interleukin (IL)-1β primed (MSCp) treatment in a context of HS- induced organ injury.MethodsRats underwent fixed pressure HS and were treated with allogenic MSCn or MSCp. Liver and kidney injuries were evaluated 6h later by histological and biochemical analysis. Whole blood was collected to measure leukocytes phenotypes. Then, in vitro characterization of MSCn or MSCp was carried out.ResultsPlasma Creatinine, blood urea nitrogen and Cystatin C were decrease by MSCp infusion as well as kidney injury molecule (KIM)-1 on histological kidney sections. Transaminases, GGT and liver histology were normalized by MSCp. Systemic cytokines (IL-6 and IL-10) as well as CD80, 86 and PD-1/PDL-1 axis were decreased by MSCp on monocytes and T lymphocytes. In vitro, MSCp showed higher level of secreted immunomodulatory molecules than MSCn.ConclusionAn early administration of MSCp moderates HS-induced kidney and liver injury. IL-1β priming improves MSC efficiency by promoting their immunomodulatory activity. These data provide proof of concept that MSCp could be a therapeutic tool to prevent the appearance of organs injury following HS.
CDK5RAP3 as a Novel Biomarker Signature Predicting Survival and Adjuvant Chemotherapeutic benefit in Gastric Cancer
Authors: Wang Jia-Bin , Gao You-Xin , Lian Ning-Zi , Ma Yu-Bin , Li Ping , Lin Jian-Xian , Lu Jun , Chen Qi-Yue , Cao Long-Long , Lin Mi , Liu Li-Chao , Tu Ru-Hong , Lin Ju-Li , Huang Ze-Ning , Zheng Hua-Long , Xie Jian-Wei , Zheng Chao-Hui , Huang Chang-ming 
Published: December 2020

Abstract
Background: We previously demonstrated that CDK5RAP3 acts as a tumour suppressor in gastric cancer through negative regulation of the Wnt/β-catenin signalling pathway, but its function in chemotherapeutic responsiveness of gastric cancer has not been investigated. In this study, we aimed to examine the clinical significance of CDK5RAP3 to predict chemotherapeutic responsiveness in gastric cancer.Methods: A collection of 188 pairs of tumour tissue microarray specimens from Fujian Medical University were employed for the discovery set, and 310 tumour tissue samples of gastric cancer patients were employed for the internal validation set. Eight-five tumour tissue samples from Qinghai University Hospital were used as the external validation set 1. Transcriptomic and clinical data of 299 gastric cancer patients from TCGA were used as the external validation set 2. CDK5RAP3 expression, microsatellite instability (MSI) status, and tumour-infiltrating lymphocytes (TIL) were examined with immunohistochemistry. Clinical outcomes of patients were compared with Kaplan-Meier curves and the Cox model.Results: In a multi-centre evaluation, increased CDK5RAP3 indication of better prognosis depends mainly on MSI-L/MSS status or TILhigh. High CDK5RAP3 expression predicts sensitive therapeutic responsiveness to postoperative adjuvant chemotherapy in gastric cancer. In a stratification analysis based on CDK5RAP3 combined with TIL or MSI status, patients with CKD5RAP3low TILlow showed no significant difference in prognosis after receiving chemotherapy, whereas patients with CKD5RAP3low TILhigh, CKD5RAP3high TILlow, and CKD5RAP3high TILhigh had better responsiveness to chemotherapy. In addition, patients with CKD5RAP3high MSI-L/MSS status benefitted the most from adjuvant chemotherapy among all patients evaluated. Conclusions: CKD5RAP3 can be used as an effective marker to evaluate individualized chemotherapy regimens in gastric cancer patients dependent on their TIL and MSI status.
Predictors of modern contraceptive use among women in Meru and Arusha districts in Northern Tanzania
Authors: Msuya Sia Emmanuel , Amour Caroline , Mahande Michael J , Manongi Rachel N , Makuwani Ahmad , Farah Amina , Elewonibi Bilikisu , Shah Iqbal 
Published: December 2020

Abstract
Background: Contraceptive use reported to avert more than 1 million maternal deaths in Sub-Saharan Africa due to decline in fertility rate and thus help to achieve MDG 4 and 5. Therefore, this study aims to determine the prevalence of modern contraceptive use, describe common methods used, source of methods and factors that predict contraceptive use among women of reproductive age (WRA) in Arusha City and Meru District Council (DC) in northern Tanzania. Methodology: The study is based on data from a cross-sectional survey conducted from January – May 2018. The study enrolled women aged 16-44 years from five selected wards in each district. Interviews were conducted at participant’s homes using electronic data collection system. Descriptive and analytical statistics were used to analyze the data.Results: A total of 3,938 women were enrolled, of whom 3,658 were sexually active at the time of survey. The mean age of 3,658 women was 30.5 (SD 7.3) years. The prevalence of modern contraceptive use (mCPR) was 47%, with no significant difference between Arusha City (47%) and Meru DC (44%). Implants (26.9%), injectables (21.5%) and pills (13.0%) were three of the common contraceptives currently used among the users. Public health facilities were the main source (72.6%) of contraceptive methods. Married/cohabiting women were significantly using modern method of contraceptives compared to single women (Adjusted Prevalence Ratio [aPR = 2.1) as were women with two or more children than others (aPR=1.5). Cost, distance, and time due to busy schedules were key challenges to access family planning (FP) services as reported by the women. Conclusion: Nearly half of WRA in the two districts were using modern contraceptives. Implants have become the most common method of contraceptives than injectables and pills. Health management teams in the two districts will need to come with strategies for improving use of other modern FP methods and improving community-based availability of contraceptive services to contribute in improving mCPR to the 60% target.