bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Congo Basin ethnographic analogue of pre-Columbian Amazonian raised fields shows the ephemeral legacy of organic matter management
Authors: Rodrigues Leonor , Sprafke Tobias , Bokatola Moyikola Carine , Barthès Bernard G. , Bertrand Isabelle , Comptour Marion , Rostain Stéphen , Yoka Joseph , McKey Doyle 
Published: July 2020

AbstractThe functioning and productivity of pre-Columbian raised fields (RFs) and their role in the development of complex societies in Amazonian savannas remain debated. RF agriculture is conducted today in the Congo Basin, offering an instructive analogue to pre-Columbian RFs in Amazonia. Our study of construction of present-day RFs documents periodic addition of organic matter (OM) during repeated field/fallow cycles. Field investigations of RF profiles supported by spectrophotometry reveal a characteristic stratigraphy. Soil geochemistry indicates that the management of Congo RFs improves soil fertility for a limited time when they are under cultivation, but nutrient availability in fallow RFs differs little from that in uncultivated reference topsoils. Furthermore, examination of soil micromorphology shows that within less than 40 years, bioturbation almost completely removes stratigraphic evidence of repeated OM amendments. If Amazonian RFs were similarly managed, their vestiges would thus be unlikely to show traces of such management centuries after abandonment. These results call into question the hypothesis that the sole purpose of constructing RFs in pre-Columbian Amazonia was drainage.
Nitrative DNA damage in lung epithelial cells exposed to indium nanoparticles and indium ions
Authors: Ahmed Sharif , Kobayashi Hatasu , Afroz Tahmina , Ma Ning , Oikawa Shinji , Kawanishi Shosuke , Murata Mariko , Hiraku Yusuke 
Published: July 2020

AbstractIndium compounds have been widely used in manufacturing displays of mobile phones, computers and televisions. However, inhalation exposure to indium compounds causes interstitial pneumonia in exposed workers and lung cancer in experimental animals. 8-Nitroguanine (8-nitroG) is a mutagenic DNA lesion formed under inflammatory conditions and may participate in indium-induced carcinogenesis. In this study, we examined 8-nitroG formation in A549 cultured human lung epithelial cells treated with indium compounds, including nanoparticles of indium oxide (In2O3) and indium-tin oxide (ITO), and indium chloride (InCl3). We performed fluorescent immunocytochemistry to examine 8-nitroG formation in indium-exposed A549 cells. All indium compounds significantly increased 8-nitroG formation in A549 cells at 5 ng/ml after 4 h incubation. 8-NitroG formation was largely reduced by 1400 W, methyl-β-cyclodextrin (MBCD) and monodansylcadaverine (MDC), suggesting the involvement of nitric oxide synthase and endocytosis. 8-NitroG formation in A549 cells was also largely suppressed by small interfering RNA (siRNA) for high-mobility group box-1 (HMGB1), receptor for advanced glycation and end products (AGER, RAGE) and Toll-like receptor 9 (TLR9). These results suggest that indium compounds induce inflammation-mediated DNA damage in lung epithelial cells via the HMGB1-RAGE-TLR9 pathway. This mechanism may contribute to indium-induced genotoxicity in the respiratory system.
Spittlebug damage on tropical grass and its impact in pasture-based beef production systems
Authors: Congio Guilhermo Francklin Souza , de Almeida Pedro Castro , Barreto Tadeu Ruzza , Tinazo Vitor Afonso , da Silva Tiago Alves Corrêa Carvalho , Costa Diogo Fleury Azevedo , Corsi Moacyr 
Published: July 2020

AbstractSpittlebugs are the main pest of tropical pastures and Marandu palisade grass (Urochloa brizantha cv. Marandu) is the most representative cultivated pasture in the tropics. Our objective was to characterize Marandu palisade grass responses subjected to Mahanarva (Hemiptera: Cercopidae) attack and to estimate the losses in terms of beef production from pasture-based systems. A set of five experiments were carried out. Three consecutive years of monitoring showed that Mahanarva spittlebugs increased their abundance after first rains with three to four peaks throughout the wet season. A decrease of 66% on herbage yield was observed in the greenhouse trial, with an average decrease of 61% on pools of calcium, magnesium, phosphorus, sulfur, potassium, crude protein, neutral-detergent fiber and in vitro digestible dry matter of Marandu palisade grass. Results from field experiments corroborated with greenhouse trial showing decreases on herbage yield varying from 31 to 43% depending on level of fertilization and grazing severity of Marandu palisade grass. Finally, an unprecedented 154-ha field experiment indicated that Mahanarva decreases 74% the beef productivity (i.e. kg body weight ha−1) of Nellore heifers grazing Marandu palisade grass.
Disorganization of chondrocyte columns in the growth plate does not aggravate experimental osteoarthritis in mice
Authors: Lamuedra Ana , Gratal Paula , Calatrava Lucía , Ruiz-Perez Víctor Luis , Largo Raquel , Herrero-Beaumont Gabriel 
Published: July 2020

AbstractOsteoarthritis (OA) is a multifactorial joint disease mainly affecting articular cartilage (AC) with a relevant biomechanical component. During endochondral ossification growth plate (GP) chondrocytes arrange in columns. GPs do not ossify in skeletally mature rodents. In neonatal mice, an altered joint loading induces GP chondrocyte disorganization. We aimed to study whether experimental OA involves GP disorganization in adult mice and to assess if it may have additional detrimental effects on AC damage. Knee OA was induced by destabilization of the medial meniscus (DMM) in wild-type (WT) adult mice, and in Tamoxifen-inducible Ellis-van-Creveld syndrome protein (Evc) knockouts (EvccKO), used as a model of GP disorganization due to Hedgehog signalling disruption. Chondrocyte column arrangement was assessed in the tibial GP and expressed as Column Index (CI). Both DMM-operated WT mice and non-operated-EvccKO showed a decreased CI, indicating GP chondrocyte column disarrangement, although in the latter, it was not associated to AC damage. The most severe GP chondrocyte disorganization occurred in DMM-EvccKO mice, in comparison to the other groups. However, this altered GP structure in DMM-EvccKO mice did not exacerbate AC damage. Further studies are needed to confirm the lack of interference of GP alterations on the analysis of AC employing OA mice.
The metasomatic enrichment of Li in psammopelitic units at San José-Valdeflórez, Central Iberian Zone, Spain: a new type of lithium deposit
Authors: Pesquera Alfonso , Roda-Robles Encarnación , Gil-Crespo Pedro P. , Valls David , Ruiz José Torres 
Published: July 2020

AbstractA new type of Li mineralisation in hard rock has been found to occur in the Valdeflórez area (Cáceres, Spain), where there is 111.3 Mt of resources and a mean value of 0.61 wt% of Li2O. Lithium is mainly held by very fine-grained micas, important constituents of Ordovician psammopelitic rocks belonging to the Palaeozoic metasedimentary sequence of the Cáceres syncline. The mineralised zone has an elliptical surface shape with a dimension of ~ 700 × 500 m. Lithium-bearing rocks show a characteristic layered appearance, in which light grey quartz-micaceous laminae < 1 mm to some centimetres in thickness, with a variable ratio of quartz to mica, alternate with fine to very fine-grained, dark grey to black tourmalinite laminae parallel to the regional foliation. Subvertical quartz + (montebrasite)-veins that cut the regional foliation at an extremely high angle are also common in this area. Mineralisation and the associated veins are likely to be linked to the intrusion of the nearby Cabeza de Araya pluton. The infiltration of granite-derived Li-, F-, B- and P-rich aqueous fluids into the host rocks through fractures related to shearing processes is considered to be the cause of the formation of Li-rich micas and intense tourmalinisation at the expense of pre-existing phyllosilicates.
Lysophosphatidic acid modulates ovarian cancer multicellular aggregate assembly and metastatic dissemination
Authors: Klymenko Yuliya , Bos Brandi , Campbell Leigh , Loughran Elizabeth , Liu Yueying , Yang Jing , Kim Oleg , Stack M. Sharon 
Published: July 2020

AbstractEpithelial ovarian cancer (EOC) metastasis occurs by exfoliation of cells and multicellular aggregates (MCAs) from the tumor into the peritoneal cavity, adhesion to and retraction of peritoneal mesothelial cells and subsequent anchoring. Elevated levels of lysophosphatidic acid (LPA) have been linked to aberrant cell proliferation, oncogenesis, and metastasis. LPA disrupts junctional integrity and epithelial cohesion in vitro however, the fate of free-floating cells/MCAs and the response of host peritoneal tissues to LPA remain unclear. EOC MCAs displayed significant LPA-induced changes in surface ultrastructure with the loss of cell surface protrusions and poor aggregation, resulting in increased dissemination of small clusters compared to untreated control MCAs. LPA also diminished the adhesive capacity of EOC single cells and MCAs to murine peritoneal explants and impaired MCA survival and mesothelial clearance competence. Peritoneal tissues from healthy mice injected with LPA exhibited enhanced mesothelial surface microvilli. Ultrastructural alterations were associated with restricted peritoneal susceptibility to metastatic colonization by single cells as well as epithelial-type MCAs. The functional consequence is an LPA-induced dissemination of small mesenchymal-type clusters, promoting a miliary mode of peritoneal seeding that complicates surgical removal and is associated with worse prognosis.
Nusinersen ameliorates motor function and prevents motoneuron Cajal body disassembly and abnormal poly(A) RNA distribution in a SMA mouse model
Authors: Berciano María T. , Puente-Bedia Alba , Medina-Samamé Almudena , Rodríguez-Rey José C. , Calderó Jordi , Lafarga Miguel , Tapia Olga 
Published: July 2020

AbstractSpinal muscular atrophy (SMA) is a devastating autosomal recessive neuromuscular disease characterized by degeneration of spinal cord alpha motor neurons (αMNs). SMA is caused by the homozygous deletion or mutation of the survival motor neuron 1 (SMN1) gene, resulting in reduced expression of SMN protein, which leads to αMN degeneration and muscle atrophy. The majority of transcripts of a second gene (SMN2) generate an alternative spliced isoform that lacks exon 7 and produces a truncated nonfunctional form of SMN. A major function of SMN is the biogenesis of spliceosomal snRNPs, which are essential components of the pre-mRNA splicing machinery, the spliceosome. In recent years, new potential therapies have been developed to increase SMN levels, including treatment with antisense oligonucleotides (ASOs). The ASO-nusinersen (Spinraza) promotes the inclusion of exon 7 in SMN2 transcripts and notably enhances the production of full-length SMN in mouse models of SMA. In this work, we used the intracerebroventricular injection of nusinersen in the SMN∆7 mouse model of SMA to evaluate the effects of this ASO on the behavior of Cajal bodies (CBs), nuclear structures involved in spliceosomal snRNP biogenesis, and the cellular distribution of polyadenylated mRNAs in αMNs. The administration of nusinersen at postnatal day (P) 1 normalized SMN expression in the spinal cord but not in skeletal muscle, rescued the growth curve and improved motor behavior at P12 (late symptomatic stage). Importantly, this ASO recovered the number of canonical CBs in MNs, significantly reduced the abnormal accumulation of polyadenylated RNAs in nuclear granules, and normalized the expression of the pre-mRNAs encoding chondrolectin and choline acetyltransferase, two key factors for αMN homeostasis. We propose that the splicing modulatory function of nusinersen in SMA αMN is mediated by the rescue of CB biogenesis, resulting in enhanced polyadenylated pre-mRNA transcription and splicing and nuclear export of mature mRNAs for translation. Our results support that the selective restoration of SMN expression in the spinal cord has a beneficial impact not only on αMNs but also on skeletal myofibers. However, the rescue of SMN expression in muscle appears to be necessary for the complete recovery of motor function.
Cochlea-inspired design of an acoustic rainbow sensor with a smoothly varying frequency response
Authors: Karlos Angelis , Elliott Stephen J. 
Published: July 2020

AbstractA number of physical arrangements for acoustic rainbow sensors have been suggested, where the aim is to separate different frequency components into different physical locations along the sensor. Although such spatial discrimination has been achieved with several designs of sensor, the resulting frequency responses at a given position along the sensor are generally not smoothly varying. In contrast, the cochlea provides an interesting natural example of a rainbow sensor, which has an exponential frequency distribution and whose response does vary smoothly with frequency. The design of a rainbow sensor is presented that has a number of discrete resonators and an exponential frequency distribution. We discuss the conditions for a smoothly varying frequency response in such a sensor, as part of a broader design strategy. It is shown that the damping within the resonators determines the trade-off between the frequency resolution and the number of elements required to achieve a smooth response. The connection is explained between this design and that of an effective acoustic absorber. The finite number of hair cells means that the cochlea itself can be thought of as being composed of discrete units and the conditions derived above are compared with those that are observed in the cochlea.
Wheat Myo-inositol phosphate synthase influences plant growth and stress responses via ethylene mediated signaling
Authors: Sharma Naveen , Chaudhary Chanderkant , Khurana Paramjit 
Published: July 2020

AbstractL-myo-inositol phosphate synthase (MIPS; EC 5.5.1.4) is involved in abiotic stress tolerance, however its disruption and overexpression has also been associated with enhanced tolerance to pathogens. The molecular mechanism underlying the role ofMIPSin growth, immunity and abiotic stress tolerance remains uncharacterized. We explore the molecular mechanism ofMIPSaction during growth and heat stress conditions. We raised and characterized theTaMIPSover-expressing rice transgenics which showed a reduced reproductive potential. Transcriptome analysis of overexpression transgenics revealed the activation of ET/JA dependent immune response. Pull-down analysis revealed the interaction of TaMIPS-B with ethylene related proteins. Our results suggest an essential requirement ofMIPSfor mediating the ethylene response and regulate the growth. A model is proposed outlining how fine tuning ofMIPSregulate growth and stress tolerance of the plant.
Glucocorticoid receptor complexes form cooperatively with the Hsp90 co-chaperones Pp5 and FKBPs
Authors: Kaziales Anna , Barkovits Katalin , Marcus Katrin , Richter Klaus 
Published: July 2020

AbstractThe function of steroid receptors in the cell depends on the chaperone machinery of Hsp90, as Hsp90 primes steroid receptors for hormone binding and transcriptional activation. Several conserved proteins are known to additionally participate in receptor chaperone assemblies, but the regulation of the process is not understood in detail. Also, it is unknown to what extent the contribution of these cofactors is conserved in other eukaryotes. We here examine the reconstituted C. elegans and human chaperone assemblies. We find that the nematode phosphatase PPH-5 and the prolyl isomerase FKB-6 facilitate the formation of glucocorticoid receptor (GR) complexes with Hsp90. Within these complexes, Hsp90 can perform its closing reaction more efficiently. By combining chemical crosslinking and mass spectrometry, we define contact sites within these assemblies. Compared to the nematode Hsp90 system, the human system shows less cooperative client interaction and a stricter requirement for the co-chaperone p23 to complete the closing reaction of GR·Hsp90·Pp5/Fkbp51/Fkbp52 complexes. In both systems, hormone binding to GR is accelerated by Hsp90 alone and in the presence of its cofactors. Our results show that cooperative complex formation and hormone binding patterns are, in many aspects, conserved between the nematode and human systems.
Giant excitonic absorption and emission in two-dimensional group-III nitrides
Authors: Prete Maria Stella , Grassano Davide , Pulci Olivia , Kupchak Ihor , Olevano Valerio , Bechstedt Friedhelm 
Published: July 2020

AbstractAbsorption and emission of pristine-like semiconducting monolayers of BN, AlN, GaN, and InN are systematically studied by ab-initio methods. We calculate the absorption spectra for in-plane and out-of-plane light polarization including quasiparticle and excitonic effects. Chemical trends with the cation of the absorption edge and the exciton binding are discussed in terms of the band structures. Exciton binding energies and localization radii are explained within the Rytova-Keldysh model for excitons in two dimensions. The strong excitonic effects are due to the interplay of low dimensionality, confinement effects, and reduced screening. We find exciton radiative lifetimes ranging from tenths of picoseconds (BN) to tenths of nanoseconds (InN) at room temperature, thus making 2D nitrides, especially InN, promising materials for light-emitting diodes and high-performance solar cells.
Cystathionine β-synthase deficiency: different changes in proteomes of thrombosis-resistant Cbs−/− mice and thrombosis-prone CBS−/− humans
Authors: Sikora Marta , Lewandowska Izabela , Marczak Łukasz , Bretes Ewa , Jakubowski Hieronim 
Published: July 2020

AbstractCystathionine β-synthase (CBS)-deficient patients are prone to vascular thrombosis. In contrast, Cbs−/− mice show no abnormalities in blood coagulation. To identify molecular basis underlying these disparately different thrombotic phenotypes, we analyzed plasma proteomes of Cbs−/− vs. Cbs+/+ mice (8-month-old, 12/group, sex-matched) and CBS−/− vs. CBS+/+ humans (37 ± 7-year-old, 10–14/group, sex-matched) using label-free mass spectrometry. We identified 117 and 41 differentiating plasma proteins in Cbs−/− mice and CBS−/− humans, respectively. Twenty-one proteins were shared between CBS−/− humans and Cbs−/− mice, with sixteen changed in the opposite direction. Proteins involved in blood coagulation and complement/coagulation cascades represented a greater fraction of the differentiating proteins in CBS−/− patients (51%) than in Cbs−/− mice (21%). Top canonical pathways, identified by Ingenuity Pathways Analysis, such as LXR/RXR, FXR/RXR activation (− log[P-value] = 30–31) and atherosclerosis signaling (− log[P-value] = 10–11) were similarly affected in Cbs−/− mice and CBS−/− humans. The Coagulation System was affected stronger in CBS−/− humans than in Cbs−/− mice (− log[P-value] = 15 vs. 10, respectively) while acute phase response and complement system were affected stronger in Cbs−/− mice (− log[P-value] = 33 and 22, respectively) than in humans (− log[P-value] = 22 and 6, respectively). Other pathways, including IL-7 signaling and B cell development were affected only in Cbs−/− mice. Taken together, our findings suggest that differences in these processes, in particular in the Coagulation System, could account for the thrombotic phenotype in CBS−/− patients and the absence of thrombosis in Cbs−/− mice. Overall, our findings suggest that Cbs−/− mice have a better adaptive response to protect from prothrombotic effects of hyperhomocysteinemia than CBS−/− humans.
Broadband thin sound absorber based on hybrid labyrinthine metastructures with optimally designed parameters
Authors: Gao Yong-xin , Lin Yuan-peng , Zhu Yi-fan , Liang Bin , Yang Jing , Yang Jun , Cheng Jian-chun 
Published: July 2020

AbstractBroadband acoustic absorbers with thin thickness are highly desired in practical situations such as architectural acoustics, yet it is still challenging to achieve high absorption by using structure with limited thickness. Here we report the theoretical optimal design, numerical simulation and experimental demonstration of a planar acoustic absorber capable of producing broadband sound absorption with deep-subwavelength thickness. The mechanism is that, we use a hybrid design of individual unit cell comprising multiple resonators with a coiled configuration for expanding the working bandwidth and downscaling the resulting device, and, on the other hand, the geometries of the constituent resonance elements are optimally designed by using genetic algorithm. Based on an analytical formula we derive for an efficient prediction of the absorption efficiency, the optimization process is accelerated and gives rise to an optimally maximized amount of absorbed energy with limited device thickness. As a result, the proposed absorber features planar profile, broad bandwidth, wide absorbing angle (the absorber works well when the incident angle of sound wave reaches 60°) and thin thickness (< 1/25 wavelength). In addition, the proposed scheme does not rely on extra sound-absorptive materials or the type of constituent solid material, which significantly simplifies the sample fabrication and improves the application potential of resulting device. The measured data agree well with the theoretical predictions, showing high sound absorption in the prescribed frequency range. We envision our design to further improve the performance of acoustic absorbers and find applications in practical situations in need of elimination of broadband acoustic waves within limited spaces.
Effect of rice straw and swine manure biochar on N2O emission from paddy soil
Authors: Yang Zhanbiao , Yu Yi , Hu Rujing , Xu Xiaoxun , Xian Junren , Yang Yuanxiang , Liu Lixia , Cheng Zhang 
Published: July 2020

AbstractWe analyzed the effects of rice straw biochar (RSBC) and swine manure biochar (SMBC) on N2O emission from paddy soil. The biochars were added to soil at the rates of 1% and 5% (w/w), and N2O emission, soil properties and soil enzyme activities were determined at the elongation, heading and maturation stages of rice growth. The N2O flux started within 2 h of adding the biochar, and decreased significantly thereafter during the three growth stages. The cumulative N2O emission was suppressed by 45.14–73.96% following biochar application, and 5% SMBC resulted in the lowest cumulative emission. In addition, biochar application significantly increased soil pH, soil organic carbon (SOC), NO3− levels and urease activity, and decreased soil NH4+ and nitrate reductase activity. Regression analysis indicated that cumulative N2O emission was correlated positively to NH4+, and negatively to soil pH, SOC and NO3−. SEM further revealed that biochar application weakened the denitrification process, and the NH4+ level had the most significant impact on N2O emission. Taken together, RSBC and SMBC regulated the nitrogen cycle in paddy soil and mitigated N2O emission by increasing soil pH, decreasing nitrate reductase activity and NH4+ content.