bluuebunny/crossref_metadata_2025_split huggingface dataset:
Integrative proteomics reveals exceptional diversity and versatility of mammalian humoral immunity
Authors: Xiang Yufei , Sang Zhe , Bitton Lirane , Xu Jianquan , Liu Yang , Schneidman-Duhovny Dina , Shi Yi 
Published: August 2020

SummaryThe humoral immune response is essential for the survival of mammals. However, we still lack a systematic understanding of the specific serologic antibody repertoire in response to an antigen. We developed a proteomic strategy to survey, at an unprecedented scale, the landscapes of antigen-engaged, serum-circulating repertoires of camelid heavy-chain antibodies (hcAbs). The sensitivity and robustness of this technology were validated using three antigens spanning orders of magnitude in immune response; thousands of divergent, high-affinity hcAb families were confidently identified and quantified. Using high-throughput structural modeling, cross-linking mass spectrometry, mutagenesis, and deep learning, we mapped and analyzed the epitopes of > 100,000 antigen-antibody complexes. Our results revealed a surprising diversity of high-affinity hcAbs for specific antigen binding on a variety of dominant epitopes. hcAbs perfect both shape and charge complementarity to target challenging antigens specifically; they can rapidly evolve to recognize a conserved, promiscuous cellular protein interaction interface, unraveling the convergent force that drives protein-protein interactions.
Cryo-EM Structures of HIV-1 trimer bound to CD4-mimetics M48U1 and BNM-III-170 adopt a CD4-bound open conformation
Authors: Jette Claudia A. , Barnes Christopher O. , Kirk Sharon M. , Melillo Bruno , Smith Amos B. , Bjorkman Pamela J. 
Published: August 2020

AbstractHuman Immunodeficiency Virus-1 (HIV-1), the causative agent of AIDS, impacts millions of people. Entry into target cells is mediated by the HIV-1 envelope (Env) glycoprotein interacting with host receptor CD4, which triggers conformational changes allowing binding to a coreceptor and subsequent membrane fusion. Small molecule or peptide CD4-mimetic drugs mimic CD4’s Phe43 interaction with Env by inserting into the conserved Phe43 pocket on Env subunit gp120. Here, we present single-particle cryo-EM structures of CD4-mimetics BNM-III-170 and M48U1 bound to a BG505 native-like Env trimer plus the CD4-induced antibody 17b at 3.7Å and 3.9Å resolution, respectively. CD4-mimetic-bound BG505 exhibits canonical CD4-induced conformational changes including trimer opening, formation of the 4-stranded gp120 bridging sheet, displacement of the V1V2 loop, and formation of a compact and elongated gp41 HR1C helical bundle. We conclude that CD4-induced structural changes on both gp120 and gp41 Env subunits are induced by binding to the gp120 Phe43 pocket.
Emergence of Non-Canonical Parvalbumin-Containing Interneurons in Hippocampus of a Murine Model of Type I Lissencephaly
Authors: Ekins Tyler G. , Mahadevan Vivek , Zhang Yajun , D’Amour James A. , Petros Timothy , McBain Chris J. 
Published: August 2020

ABSTRACTType I lissencephaly is a neuronal migration disorder caused by haploinsuffiency of theLIS1 geneand is characterized in humans by agyria, mislamination of brain structures, developmental delays, and epilepsy. Here, we investigate the impact ofLIS1mutation on the cellular migration, morphophysiology, microcircuitry and genomics of mouse hippocampal CA1 parvalbumin-containing inhibitory interneurons (PV+INTs). We find that WT PV+INTs consist of two physiological subtypes (80% fast-spiking (FS), 20% non-fast-spiking (NFS)) and four morphological subtypes (basket, axo-axonic, bistratified, radiatum-targeting). We also discover that cell-autonomous mutations within interneurons disrupts morphological development of PV+INTs and results in the emergence of a non-canonical “intermediate spiking (IS)” subset of PV+INTs. In the GlobalLis mutant, IS/NFS cells become the dominant PV+INT subtypes (56%) and the percentage of FS cells shrinks to 44%. We also find that IS/NFS cells are prone to entering depolarizing block, causing them to temporarily lose the ability to initiate action potentials and control network excitation, potentially promoting seizures. Finally, single-cell nuclear RNAsequencing of PV+INTs revealed several misregulated genes related to morphogenesis, cellular excitability, and synapse formation.
Stable isotope informed genome-resolved metagenomics uncovers potential trophic interactions in rhizosphere soil
Authors: Starr Evan P. , Shi Shengjing , Blazewicz Steven J. , Koch Benjamin J. , Probst Alexander J. , Hungate Bruce A. , Pett-Ridge Jennifer , Firestone Mary K. , Banfield Jillian F. 
Published: August 2020

AbstractThe functioning, health, and productivity of soil is intimately tied to a complex network of interactions, particularly in plant root-associated rhizosphere soil. We conducted a stable isotope-informed, genome-resolved metagenomic study to trace carbon fromAvena fatuagrown in a13CO2atmosphere into soil. We collected paired rhizosphere and non-rhizosphere soil at six and nine weeks of plant growth and extracted DNA that was then separated by density using gradient centrifugation. Thirty-two fractions from each sample were grouped by density, sequenced, assembled, and binned to generate 55 unique microbial genomes that were >70% complete. The complete 18S rRNA sequences of several micro-eukaryotic bacterivores and fungi were enriched in13C. We generated several circularized bacteriophage (phage) genomes, some of which were the most labelled entities in the rhizosphere. CRISPR locus targeting connected one of these phage to a Burkholderiales host predicted to be a plant pathogen. Another highly labeled phage is predicted to replicate in aCatenulispora sp., a possible plant growth-promoting bacterium. We searched the genomes for traits known to be used in interactions involving bacteria, micro-eukaryotes and plant roots and found that heavily isotopically-labeled bacteria have the ability to modulate plant signaling hormones, possess numerous plant pathogenicity factors, and produce toxins targeting micro-eukaryotes. Overall,13C stable isotope-informed genome-resolved metagenomics revealed that very active bacteria often have the potential for strong interactions with plants and directly established that phage can be important agents of turnover of plant-derived carbon in soil.
Estimating the density of small population of leopardPanthera pardususing multi-session photographic□sampling data
Authors: Farhadinia Mohammad S. , Behnoud Pouyan , Hobeali Kaveh , Mousavi Seyed Jalal , Hosseini-Zavarei Fatemeh , Gholikhani Navid , Akbari Hasan , Eslami Morteza , Moghadas Peyman , Macdonald David W. 
Published: August 2020

AbstractWest Asian drylands host a number of threatened large carnivores, including the leopard(Panthera pardus)which is limited to spatially scattered landscapes with generally low primary productivity. While conservation efforts have focused on these areas for several decades, reliable population density estimates are missing. Spatially-explicit capture-recapture (SECR) methodology, incorporating animal movement in density estimates, is widely used to monitor populations of large carnivores. We employed multi-session SECR modeling to estimate the density of a small population of leopard (Panthera pardus) in a mountainous stretch surrounded by deserts in central Iran. During 6724 camera trap nights, we detected eight and five independent leopards in 2012 and 2016 sessions, respectively. The top performing model demonstrated density estimates of 1.6 (95% CI = 0.9-2.9) and 1.0 (95% CI = 0.6-1.6) independent leopards/100 km2in 2012 and 2016, respectively. Both sex and season had substantial effects on spatial scale (σ), with larger movements for males and during winter. Currently available estimates in arid regions represent some of the lowest densities across the leopard global range. These small populations are vulnerable to demographic stochasticity. Monitoring temporal changes in population density and composition can inform conservation priorities.
DNA-RNA hybrid (R-loop): from a unified picture of the mammalian telomere to the genome-wide
Authors: Rassoulzadegan Minoo , Sharifi-Zarchi Ali , Kianmehr Leila 
Published: August 2020

SummaryLocal three-stranded DNA-RNA hybrid regions of the genomes (R-loops) have been detected either by binding of a monoclonal antibody (DRIP assay) or by enzymatic recognition by RNaseH. Such a structure has been postulated for the mouse and human telomeres, clearly suggested by the identification of the complementary RNA TERRA. It was, however, evidenced by DRIP exclusively in human cancer and not in normal human nor in any mouse cell. Based on the observation that in several fractionation procedures, DNA-RNA hybrids copurify with double-stranded DNA, we developed a preparative approach that allows detection of stable DNA-RNA triplexes in a complex genome, their physical isolation and their RNA nucleotide sequence. We then define in the normal mouse and human genomes the notion of terminal R-loop complexes including TERRA molecules synthesized from local promoters of every chromosome. In addition to the telomeric structures since, the finding of the RNA peak, applied in addition to the whole murine sperm genomes, has highlighted a reproducible profile of the R-loop complexes from entire genome suggesting a defined profile for a future generation.
Developing insights into the link between art and tourism through the value co-creation lens
Authors: Gökbulut Özdemir Özge , Fillis Ian , Baş Collins Ayşe 
Published: August 2020

PurposeThe aim of the study is to gain insight into the link between art and tourism from a value co-creation perspective. This link is discussed with the help of the arts marketing, art tourism and value co-creation literature. The role of art in tourism and the role of cultural places in arts marketing are also evaluated.Design/methodology/approachFocussing on two cultural heritage sites in Turkey, Zeugma and Göbeklitepe, a qualitative study was undertaken in order to determine the value creation and co-creation processes occurring from the art–tourism contexts based on comparative case study analysis. In-depth semi-structured interviews were conducted with three groups of actors. Motivation, expectation and stakeholder experiences were the main themes explored.FindingsThe findings of the study relate to the role of the co-creation process. Marketing art in alternative places creates value in closing the gap between art and society through the use of related fields such as culture and heritage. In terms of cultural value, the paper identifies the reconnection with cultural heritage through contemporary art. This is a way of looking at culture and its concepts in different time and place dimensions which make visitors more engaged with culture and its contemporary reflection through art.Research limitations/implicationsAlthough the research focusses on two Turkish art and tourism cases, future research can be extended to other countries, including the assessment of the longer-term role of similar activities.Practical implicationsAs art is a subset of culture, the people who are interested in culture and history also have the potential to be interested in art. While art impacts on cultural tourism, cultural heritage and tourism work as arts marketing tools in a co-supporting way. The coming together of art and culture has societal benefits. There are lessons for practice such as the opening of a space for contemporary art in cultural heritage museums in order to promote art to society. The museum audience is an important potential for the future of art from a market generation perspective.Originality/valueThe study contribute to arts tourism, arts marketing and value co-creation in theory and practice.
Towards developing a measure of disorganization
Authors: Herath Dinuka B. 
Published: August 2020

PurposeThis paper aims to test the hypothesized concave relationship between disorganization and individual financial performance using UK Workplace Employment Relations Study (WERS) datasets. Given there are no prior studies measuring disorganization we start with using scale items from currently validated scales, WERS, and try to determine the extent to which the current scales are applicable for measuring disorganization and subsequently highlight the limitations of current measures.Design/methodology/approachThis paper is based on the UK Workplace Employment Relations study (WERS) datasets of 2011 which is the largest publicly accessible dataset available. The datasets used were the financial performance survey (FPS) data and the management survey (MS) data with 545 unique records. Polynomial Regression was used to test the hypotheses. An aggregated index for disorganization (IV) was developed, and a production function was used to determine the individual financial performance per worker (DV).FindingsA significant linear relationship between disorganization and individual financial performance was discovered. However, this relationship was linear and did not exhibit the theorized concave relationship. The findings further indicated the need for more refined measures of disorganization and limitations of the current measures.Originality/valueWhile the study is exploratory in nature, this is the first study to date which attempts to measure disorganization in an applied setting. Thus, the work presented here is foundational to any future empirical studies on the topic. The limitations uncovered are of particular importance.
Gaining satisfaction: the role of brand equity orientation and failure type in service recovery
Authors: Ma Ke , Zhong Xin , Hou Guanghui 
Published: August 2020

PurposeThis study aims to examine the role played by brand equity orientation and failure type in service recovery. Specifically, through the lens of forgiveness, the way brand equity orientation and failure type influence consumer response to recovery is revealed.Design/methodology/approachThis research tests a novel model using data collected from 1,589 consumers in two scenario-based experimental studies. The statistical product and service solutions (SPSS) program with the PROCESS tool was used to test the mediation and moderated mediation effects.FindingsThe research findings suggest that forgiveness plays a mediation role in the relationship between recovery and satisfaction. Brand equity orientation moderates the mediation effect of forgiveness on the relationship between recovery and recovery satisfaction. In addition, failure type also plays an important role and there is a significant three-way interaction effect (service recovery × brand equity orientation × failure type) on recovery satisfaction under certain circumstances.Research limitations/implicationsBuilding on the extant literature which focuses on the cognitive process when investigating recovery and consumer reaction, this research advocates the significant role played by the psychological process, namely, the feeling of forgiveness, in explaining the effect of distinct recovery strategies on consumer satisfaction. This research also unveils the effects of brand equity orientation and failure type on recovery outcomes.Practical implicationsWhen addressing performance failure, brand equity orientation and failure type need to be identified. Businesses could develop recovery strategies to arouse consumer forgiveness, which would lead to increased recovery satisfaction. When designing recovery strategies, managers need to be mindful of the effects of brand equity orientation and failure type.Originality/valueThis research is one of the few which reveals the mediating role played by forgiveness on the effect of recovery. Taking brand equity orientation and failure type into consideration, the findings of this research provide new insights into the recovery literature.
Risk identification and modeling for blockchain-enabled container shipping
Authors: Nguyen Son , Chen Peggy Shu-Ling , Du Yuquan 
Published: August 2020

PurposeAlthough being considered for adoption by stakeholders in container shipping, application of blockchain is hindered by different factors. This paper investigates the potential operational risks of blockchain-integrated container shipping systems as one of such barriers.Design/methodology/approachLiterature review is employed as the method of risk identification. Scientific articles, special institutional reports and publications of blockchain solution providers were included in an inclusive qualitative analysis. A directed acyclic graph (DAG) was constructed and analyzed based on network topological metrics.FindingsTwenty-eight potential risks and 47 connections were identified in three groups of initiative, transitional and sequel. The DAG analysis results reflect a relatively well-connected network of identified hazardous events (HEs), suggesting the pervasiveness of information risks and various multiple-event risk scenarios. The criticality of the connected systems' security and information accuracy are also indicated.Originality/valueThis paper indicates the changes of container shipping operational risk in the process of blockchain integration by using updated data. It creates awareness of the emerging risks, provides their insights and establishes the basis for further research.
Effects of the political risk on Bitcoin return and volatility: evidence from the 2016 US presidential election
Authors: Ajmi Hechem , Arfaoui Nadia 
Published: August 2020

Purpose
This paper aims to investigate the effect of the political risk on Bitcoin return and volatility during the 2016 US pre-election and post-election periods.

Design/methodology/approach
A daily composite political risk index is calculated by using the principal component analysis and Google Trends. A quantile regression approach is adopted to assess the effect of the political risk index on Bitcoin return and volatility for both periods subject to market conditions.

Findings
Findings reveal that the political risk index tends to increase when moving from the pre-election period to the post-election one. This is mostly attributed to the new challenges faced by the new elected government. During the pre-election period, the quantiles regression shows that the political risk index negatively affects Bitcoin return when the market is bearish, whereas a positive impact on volatility is found in bearish and bullish markets. When the political situation becomes severer during the post-election period, the quantiles plots show that the increase of the political risk index leads to a significant increase of Bitcoin return, whereas Bitcoin volatility remains relatively stable. This means that Bitcoin can be adopted as a hedging tool when the political situation becomes severer.

Originality/value
Comparing to the existed studies in the field, this paper considers Google trends as a main source to assess the daily composite political risk index during the 2016 US presidential election.
The empirical reality of project management failures in the construction of social housing projects in South Africa
Authors: Amoah Christopher , Kajimo-Shakantu Kahilu , van Schalkwyk Tanya 
Published: August 2020

Purpose
The concept of government reconstruction development programme (RDP) social housing in South Africa was rolled out in 1994 after the African National Congress Government came to power when the apartheid rule was abolished. The main aim of the government was to enhance the lifestyles of the poor in society through the provision of houses that they could not afford in the open market. However, many concerns have been reported about the social housing project in terms of poor project implementation and the delivery of deliverables that do not befit the need of the end-users. This study aims to assess the flaws in the application of project management (PM) principles in the construction of these social houses.

Design/methodology/approach
A quantitative approach was adopted for the study by making use of closed- and open-ended questionnaires to collect data from 1,893 social housing inhabitants in Bloemfontein, Free State. Descriptive statistics and R programming language software were used to analyse the data collected.

Findings
The findings reveal that there was a profound failure in the application of PM principles in the construction of the social houses leading to the provision of deliverables that do not meet the needs of the beneficiaries. There are also poor project deliverables and lack of consultations that could have probably been prevented had proper PM systems been put in place by the government throughout the project lifecycle. This lack of proper PM philosophies has generated dissatisfaction among the beneficiaries leading to numerous complaints about the social housing programme.

Research limitations/implications
The survey was done in only RDP housing communities in Bloemfontein in the Free State Province of South Africa; however, the result may be applicable in other RDP housing programmes.

Practical implications
The empirical results indicate that the government has been providing houses with disregard to project objectives by not instituting an appropriate PM systems; hence, the main objective of providing befitting houses to the less privileged to enhance their living conditions has woefully failed, as the inhabitants do not see any improvement of their social standings after receiving the houses. This means the government might have wasted resources as a result of ineffective PM throughout the project implementation.

Originality/value
This study has identified PM flaws in the construction of the RDP houses, which have led to poor project deliverables. This study thus gives recommendations with regard to proper PM strategies for the implementation of the same or similar project in the future to achieve project objectives.
Perceived quality of built environment, service, satisfaction and value in use, in the context of residential buildings
Authors: Da Silva Marcelo Benetti Corrêa , Giacometti Valente Marina , Petroli Angela , Detoni Deonir , Milan Gabriel Sperandio 
Published: August 2020

PurposePost-occupancy evaluation (POE) demonstrates relevance nowadays. Because of the wide competition between construction companies, it is necessary to be assertive, so satisfied clients bring new clients through communication amongst the users. This paper aims to assist a construction company, evaluating apartment owner’s perceptions regarding the built environment, the perceived quality of services, the satisfaction and the value in use, in the context of multifamily buildings.Design/methodology/approachThis paper presents quantitative research of POE, applying confirmatory factor analysis and multiple linear regression. These methods analyse how the attributes behave in each theoretical dimension and evaluate the relationship between the dimensions of the built environment and services including customer satisfaction and value in use.FindingsThe results demonstrate that the constructs associated with management aspects such as service, cause higher levels of satisfaction and, therefore, higher value in use. The results become guidelines for constructors, supporting management and project decision-making.Research limitations/implicationsThis study’s limitation is related to the number of questionnaires applied. The survey was applied to the apartment owners in all five buildings from a constructor, considered as the entire population of the research. For that reason, there were not used a sample. Further studies are required to improve the results, even more, using the present research in a larger sample, seeking a better generalisation. Also, future studies might evaluate other types of constructions and environments.Originality/valueThis paper used marketing constructs related to satisfaction, value in use and service, applied in the construction field of multifamily residential buildings.