bluuebunny/crossref_metadata_2025_split huggingface dataset:
Kaiso regulates DNA methylation homeostasis
Authors: Kaplun D , Filonova G , Lobanova Y. , Mazur A , Zhenilo S 
Published: December 2020

ABSTRACTGain and loss of DNA methylation in cells is a dynamic process that tends to achieve an equilibrium. Many factors are involved in maintaining the balance between DNA methylation and demethylation. Previously, it was shown that methyl-DNA protein Kaiso may attract NcoR, SMRT repressive complexes affecting histone modifications. On the other hand, the deficiency of Kaiso resulted in slightly reduced methylation of ICR in H19/Igf2 locus and Oct4 promoter in mouse embryonic fibroblasts. However, nothing is known whether Kaiso may attract DNA methyltransferase to influence DNA methylation level. The main idea of this work is that Kaiso may lead to DNA hypermethylation attracting de novo DNA methyltransferases. We demonstrated that Kaiso regulates TRIM25 promoter methylation. It can form a complex with DNMT3b. BTB/POZ domain of Kaiso and ADD domain of DNA methyltransferase are essential for complex formation. Thus, Kaiso can affect DNA methylation.
Characterization of protease activity of Nsp3 from SARS-CoV-2 and itsin vitroinhibition by nanobodies
Authors: Armstrong Lee A. , Lange Sven M. , de Cesare Virginia , Matthews Stephen P. , Nirujogi Raja Sekar , Cole Isobel , Hope Anthony , Cunningham Fraser , Toth Rachel , Mukherjee Rukmini , Bojkova Denisa , Gruber Franz , Gray David , Wyatt Paul G. , Cinatl Jindrich , Dikic Ivan , Davies Paul , Kulathu Yogesh 
Published: December 2020

AbstractOf the 16 non-structural proteins (Nsps) encoded by SARS CoV-2, Nsp3 is the largest and plays important roles in the viral life cycle. Being a large, multidomain, transmembrane protein, Nsp3 has been the most challenging Nsp to characterize. Encoded within Nsp3 is the papain-like protease PLpro domain that cleaves not only the viral protein but also polyubiquitin and the ubiquitin-like modifier ISG15 from host cells. We here compare the interactors of PLpro and Nsp3 and find a largely overlapping interactome. Intriguingly, we find that near full length Nsp3 is a more active protease compared to the minimal catalytic domain of PLpro. Using a MALDI-TOF based assay, we screen 1971 approved clinical compounds and identify five compounds that inhibit PLpro with IC50s in the low micromolar range but showed cross reactivity with other human deubiquitinases and had no significant antiviral activity in cellular SARS-CoV-2 infection assays. We therefore looked for alternative methods to block PLpro activity and engineered competitive nanobodies that bind to PLpro at the substrate binding site with nanomolar affinity thus inhibiting the enzyme. Our work highlights the importance of studying Nsp3 and provides tools and valuable insights to investigate Nsp3 biology during the viral infection cycle.
TRIM28 regulates Kaiso SUMOylation
Authors: Filonova G. , Lobanova Y. , Kaplun D , Zhenilo S 
Published: December 2020

AbstractTripartite motif protein 28 (TRIM28), a universal mediator of Krüppel-associated box domain zinc fingers (KRAB-ZNFs), is known to regulate DNA methylation of many repetitive elements and several imprinted loci. TRIM28 serves as a scaffold unit that is essential for the formation of stable repressor complexes. In the present study we found that TRIM28 is a binding partner for methyl-DNA binding protein Kaiso. Kaiso is a transcription factor that belongs to the BTB/POZ -zinc finger family. Recent data suggest that deficiency of Kaiso led to reduction of DNA methylation within the imprinting control region of H19/IGF2. Thus, we hypothesized that Kaiso and TRIM28 may cooperate to control methylated genes. We demonstrated that Kaiso interacts with TRIM28 via its two domains: BTB/POZ and three zinc finger domains. When bound to Kaiso’s zinc finger domains TRIM28 weakens their interactions with methylated DNAin vitro. Specific association of TRIM28 with BTB/POZ domain causes Kaiso hyperSUMOylation. Altogether our data describe a putative role of TRIM28 as a regulator of Kaiso activity.
Nanodisc, amphipol or detergent belts in cryoEM reconstructions of membrane proteins are similar and correspond to a common ordered solvent layer
Authors: Zampieri Veronica , Gobet Alexia , Robert Xavier , Falson Pierre , Chaptal Vincent 
Published: December 2020

SummaryTo maintain membrane proteins soluble in aqueous solution, amphipathic compounds are used to shield the hydrophobic patch of their membrane insertion, which forms a belt around the protein. This hydrophobic belt is seldom looked at due to the difficulty to visualize it. Cryo-EM is now offering this possibility, where belts are visible in 3D reconstructions. We investigated membrane proteins solved in nanodiscs, amphipols or detergents to analyze whether the nature of the amphipathic compound influences the belt size in 3D reconstructions. We identified belt boundaries in map-density distributions and measured distances for every reconstruction. We showed that all the belts create on average similar reconstructions, whether they originate from the same protein, or from protein from different shapes and structures. There is no difference among detergents or types of nanodisc used. These observations illustrate that the belt observed in 3D reconstructions corresponds to the minimum ordered layer around membrane proteins.
ABPP-HT - high-throughput activity-based profiling of deubiquitylating enzyme inhibitors in a cellular context
Authors: Jones Hannah , Heilig Raphael , Fischer Roman , Kessler Benedikt M , Pinto-Fernandez Adan 
Published: December 2020

AbstractThe potency and selectivity of a small molecule inhibitor are key parameters to assess during the early stages of drug discovery. In particular, it is very informative for characterizing compounds in a relevant cellular context in order to reveal potential off-target effects and drug efficacy. Activity-based probes (ABPs) are valuable tools for that purpose, however, obtaining cellular target engagement data in a high-throughput format has been particularly challenging. Here, we describe a new methodology named ABPP-HT (high-throughput-compatible activity-based protein profiling), implementing a semi-automated proteomic sample preparation workflow that increases the throughput capabilities of the classical ABPP workflow approximately ten times while preserving its enzyme profiling characteristics. Using a panel of deubiquitylating enzyme (DUB) inhibitors, we demonstrate the feasibility of ABPP-HT to provide compound selectivity profiles of endogenous DUBs in a cellular context at a fraction of time as compared to previous methodologies.
The influence of personality and social traits on the importance of brand design of luxury brands and brand loyalty
Authors: Konjkav Monfared Amirreza , Mansouri Arefeh , Jalilian Negar 
Published: December 2020

PurposeBuyers of luxury clothing products usually place great importance on design because they can satisfy their personal needs. However, the underlying motivation for buying luxury products has not been fully understood. Therefore, identifying the factors influencing the choice of luxury products and brand loyalty can provide useful information to a better understanding of the customers' needs of these brands. In fact, in this article, we are trying to determine how personality traits (including the need for uniqueness and self-monitoring) and social traits (including self-expression and self-presentation) influence the importance of design and brand loyalty.Design/methodology/approachA questionnaire was used to collect data. The questionnaire was answered by 386 buyers of luxury clothing brands in Iran. Structural equation modeling was also used for data analysis. Data were analyzed by SPSS 19.0 and AMOS 24.0 software.FindingsThe results of this study show that people who need to be unique pay more attention to the specific designs by expressing their self-expression in their surrounding community, while self-monitors seek acceptance in the community by using common designs. Finally, the results show that the importance of design reduces customer loyalty to the brand.Originality/valueTo the best of the authors' knowledge, this study is the first one to investigate the effect of personality and social traits on the importance of luxury clothing design and brand loyalty using statistical data analysis tools in Iran.
Walkability evaluation of building circulation based on user preference
Authors: Fu Meiqing , Liu Rui , Hon Carol K.H. 
Published: December 2020

PurposeBuilding circulation has an important impact on human comfort of buildings and is one of the critical factors in building design. A quantitative walkability evaluation of building circulation can benefit both building design and operation. However, indoor walkability of building circulation is determined not only by objective path features but also by subjective user preference. How to incorporate the preference from a large group of users into the design process is still a challenging issue.Design/methodology/approachThis study proposes a participatory framework of indoor path walkability evaluation based on user preference. Hierarchical indicators are developed to objectively measure indoor path features. Furthermore, group decision-making theory is adopted to aggregate individual user preference into user common preference for determining the relative indicator weights. Finally, integrated walkability scores (IWSs) are calculated to evaluate indoor path walkability quantitatively.FindingsA total of three case scenarios demonstrate that the proposed evaluation framework provides an efficient way for designers and owners to measure user preference quantitatively, analyze building circulations based on user preference and compare the walkability of different building design schemes.Practical implicationsThe developed methods provide an efficient way for designers and owners to measure user preference quantitatively, analyze building circulations based on user preference and compare the walkability of different building design schemes.Originality/valueThis study develops a comprehensive and quantitative walkability evaluation approach that considers both objective path features and subjective user preference derived from user characteristics and walking purposes, which provides an effective way to incorporate user feedback into the building design process and operation.
Development of pedagogical competencies of the vocational teachers in Italy and Lithuania: implications of competence-based VET curriculum reforms
Authors: Tacconi Giuseppe , Tūtlys Vidmantas , Perini Marco , Gedvilienė Genute 
Published: December 2020

PurposeThe present study aims to reveal common and diverging trends in the development of pedagogical competences of vocational education and training (VET) teachers and trainers in Italy and Lithuania.Design/methodology/approachA structured survey was administered to Italian and Lithuanian teachers. The collected data were analysed and compared.FindingsResults show that there are many common challenges and problems in the development of pedagogical competencies of the VET teachers in both countries; e.g.: the marginalisation of the VET teacher's work and working conditions, especially the dissatisfying wages and poor career opportunities, and the absent or weak institutionalisation of the VET teacher's qualifications and training.Originality/valueThe emerged results can be useful for directors of VET-centres and VET-schools to manage training and pedagogical growth of teachers both in Italy and in Lithuania. Moreover, the outputs can be considered as a set of suggestions also by the policymakers both at national and European level.
Transient disturbances in a nonlocal functionally graded thermoelastic solid under Green–Lindsay model
Authors: Kumar Rajesh , Thakran Seema , Gunghas Ankush , Kalkal Kapil Kumar 
Published: December 2020

PurposeThe purpose of this study is to analyze the two-dimensional disturbances in a nonlocal, functionally graded, isotropic thermoelastic medium under the purview of the Green–Lindsay model of generalized thermoelasticity. The formulation is subjected to a mechanical load. All the thermomechanical properties of the solid are assumed to vary exponentially with the position.Design/methodology/approachNormal mode technique is proposed to obtain the exact expressions for the displacement components, stresses and temperature field.FindingsNumerical computations have been carried out with the help of MATLAB software and the results are illustrated graphically. These are also calculated numerically for a magnesium crystal-like material and illustrated through graphs. Theoretical and numerical results demonstrate that the nonlocality and nonhomogeneity parameters have significant effects on the considered physical fields.Originality/valueInfluences of nonlocality and nonhomogeneity on the physical quantities are carefully analyzed for isothermal and insulated boundaries. The present work is useful and valuable for analysis of problems involving mechanical shock, nonlocal parameter, functionally graded materials and elastic deformation.
Organisational justice and innovative behaviour: Is knowledge sharing a mediator?
Authors: K. Jnaneswar , Ranjit Gayathri 
Published: December 2020

Purpose
Encouraging employees to exhibit innovative behaviour at the workplace is the need of the hour. Prior studies reported the impact of organisational justice on innovative behaviour; however, the majority of these studies are from the Western context. Moreover, the underlying mechanism linking these variables, namely, the role of knowledge sharing, is sparse. The purpose of this paper is to examine the mediating role of knowledge sharing in the relationship between organisational justice and innovative behaviour through the lens of social exchange theory in the Indian context.

Design/methodology/approach
Mediation analysis using PROCESS macro was performed on a sample of 288 employees using three structured questionnaires. Participants were recruited from various manufacturing organisations in India. The validity of the hypothesised model was established using AMOS software.

Findings
Organisational justice impacted both knowledge sharing and innovative behaviour. It was also illustrated that knowledge sharing influenced employees’ innovative behaviour. The most important finding is the partial mediation of knowledge sharing in the organizational justice–innovative behaviour relationship.

Originality/value
This study tries to demystify the organisational justice–innovative behaviour relationship by highlighting knowledge sharing as an underlying mechanism. The existing theoretical framework that describes the effects of organisational justice is enriched.
Research progress in tourism, leisure and hospitality in Europe (1969–2018)
Authors: Mulet-Forteza Carles , Lunn Erika , Merigó José M. , Horrach Patricia 
Published: December 2020

PurposeThis study aims to present a bibliometric overview of articles published in the field of tourism, leisure and hospitality and analyzed by researchers mainly affiliated with European institutions.Design/methodology/approachThe authors conducted a bibliometric study of journals included in the Web of Science related to the field of tourism, leisure and hospitality in 2019. The review incorporates various techniques to determine the field’s structure from a scientific and intellectual perspective.FindingsThe results are valuable for several reasons. First, they will support researchers in identifying those topics with the greatest potential for advancing research in this field. Second, they will constitute an important aid in the design of new policies for journal publishers.Practical implicationsThis study can lead to advances in the tourism, leisure and hospitality field, as it identifies the publication trends of researchers who are mainly affiliated with European institutions. It also offers useful information for practitioners and academics in their endeavor to identify gaps in the extant literature and future trends.Originality/valueNo other studies have analyzed this field for a period of this length.
The role of green logistics management practices, supply chain traceability and logistics ecocentricity in sustainability performance
Authors: Agyabeng-Mensah Yaw , Afum Ebenezer , Acquah Innocent Senyo Kwasi , Dacosta Essel , Baah Charles , Ahenkorah Esther 
Published: December 2020

PurposeThe priority giving to green practices in today's competitive market has made green logistics management practices (GLMPS) a significant driver of organizational performance. The purpose of this study is to explore the influence of GLMPS, logistics ecocentricity and supply chain traceability on sustainability performance.Design/methodology/approachThe study uses structured questionnaires to gather data from 274 managers of manufacturing firms in Ghana. The partial least square structural equation modeling approach is used to analyze the data to test the proposed hypotheses.FindingsThe results obtained from the analysis indicate that GLMPS positively influence social sustainability and environmental sustainability. However, GLMPS negatively influence business performance. The results further reveal that logistics ecocentricity and supply chain traceability augment GLMPS to achieve significant improvement in both business performance and environmental sustainability through the mediation effect approach.Originality/valueThe study proposes a conceptual framework that tests the combined effect of GLMPs, logistics ecocentricity and supply chain traceability on environmental sustainability, social sustainability and business performance from the Ghanaian perspective.
Leader-member exchange and frontline employees' innovative behaviors: the roles of employee happiness and service climate
Authors: Bani-Melhem Shaker , Al-Hawari Mohd Ahmad , Quratulain Samina 
Published: December 2020

PurposeThis research primarily aims to study the role of leader-member exchange (LMX) in frontline employees' (FLEs) innovative behaviors, whereby a mediating effect of employee happiness is proposed in this relationship. The moderating effect of service climate is also examined on the indirect effect of LMX on innovative behaviors through happiness.Design/methodology/approachThe study used a sample of 303 FLEs working in various service organizations in the United Arab Emirates (UAE). Structural equation modeling was used to test the proposed model.FindingsThe findings show that LMX has a positive and significant effect on FLEs' innovative behaviors and that employee happiness is an intervening variable. Service climate moderates the indirect effect of LMX on FLEs' innovative behaviors through happiness, and the effect is stronger in a low (unsupported) service climate.Practical implicationsThe findings of this research provide prescriptive insights into the critical role of supervisory behavior in FLEs' innovative service behaviors and how positive emotions contribute to employees' willingness to innovate. Thus, these findings make a unique contribution to research in service management.Originality/valueStudies examining how and when LMX can affect FLEs' innovative behaviors are limited. These findings offer new insights into the relative importance of supervisor and organizational support (service climate) in FLEs' innovative behaviors. The interaction effect of LMX and service climate has not been previously examined along with positive employee affect (happiness) and innovative behaviors.
A hybrid reliability-centered maintenance approach for mining transportation machines: a real case in Esfahan
Authors: Jafarpisheh Rana , Karbasian Mehdi , Asadpour Milad 
Published: December 2020

PurposeThe purpose of this study is to propose a hybrid reliability-centered maintenance (RCM) approach for mining transportation machines of a limestone complex, a real case in Esfahan, Iran.Design/methodology/approachCriteria for selecting critical machines were collected within literature and selected by decision-makers (DCs), and critical machines have been identified using the preference ranking organization method for enrichment of evaluations (PROMETHEE). Also, multi-criteria decision-making (MCDM) methods were used in addition to failure mode, effects and criticality analysis (FMECA) for selecting and prioritizing high-risk failures as well as optimizing the RCM performance. More specifically, the criteria of severity, detectability and frequency of occurrence were selected for risk assessment based on the previous studies, and were weighted using the analytic hierarchy process (AHP) method. Also, the technique for order of preference by similarity to ideal solution (TOPSIS) has been applied to prioritize failures' risk. Finally, the critical failures were inserted in the RCM decision-making worksheet and the required actions were determined for them.FindingsAccording to the obtained values from PROMEHTEE method, the machine with code 739-7 was selected as the first priority and the most critical equipment. Further, based on results of TOPSIS method, the failure mode of “Lubrication hole clogging in crankpin bearing due poor quality oil,” “Deformation of main bearing due to overwork” and “The piston ring hotness due to unusual increase in the temperature of cylinder” have the highest risks among failure modes, respectively.Originality/valueRCM has been deployed in various studies. However, in the current study, a hybrid MCDM-FMECA has been proposed to cope with high-risk failures. Besides, transportation machineries are one of the most critical equipment in the mining industry. Due to noticeable costs of this equipment, effective and continuous usage of this fleet requires the implementation of proper maintenance strategy. To the best of our knowledge, there is no research which has used RCM for transportation systems in the mining sector, and therefore, the innovation of this research is employment of the proposed hybrid approach for transportation machineries in the mining industry.
Mechanical and tribological properties of waste iron scale reinforced polypropylene composite
Authors: Erdogan Azmi , Gök Mustafa Sabri , Kurşuncu Bilal , Kiraz Tayfun , Demirel Bilal 
Published: December 2020

Purpose
In this study, waste iron scale, which occurs in high amounts during steel production and contains high amounts of iron element, was used as a reinforcing material in the polypropylene (PP) matrix.

Design/methodology/approach
In the PP matrix, 33 micron-sized iron scale was added at 5%, 10%, 15% and 20% ratios. The composites were subjected to mechanical and dry sliding wear tests. The wear mechanisms occurring on the wear surfaces were determined by SEM supported by EDS. Tensile testing was performed using a tensile tester. Hardness tests were performed using a Shore-D hardness tester with ASTM-D-22 standards.

Findings
Composite reinforced with 5% iron scale showed the highest tensile strength. The addition of higher amounts of iron scale particles reduced the tensile strength of the composites compared to PP. Hardness increased from 58 to 64 Shore-D with the increase in scale content. The reinforcement of PP with iron scale increased the dry sliding wear resistance.

Originality/value
According to the authors’ knowledge, in the literature review, there was no study found on the effect of iron scale reinforcement on PP.

Peer review
The peer review history for this article is available at: https://publons.com/publon/10.1108/ILT-08-2020-0316/