bluuebunny/crossref_metadata_2025_split huggingface dataset:
REALIDAD E IMAGINARIO EN LA RELACIÓN DE GASPAR DE CARVAJAL SOBRE EL DESCUBRIMIENTO DE LA AMAZONÍA
Authors: Rosário Jocenilda Pires de Sousa do , Almeida Carlos Henrique Lopes de 
Published: June 2020

El presente trabajo se ocupa de la expedición conquistadora de Francisco de Orellana y Gonzalo Pizarro que cruzaron el Río Amazonas en 1541/1542 en busca del mítico reino de El Dorado como del País de la Canela. El artículo objetiva identificar la realidad y el imaginario presentes en la relación escrita por Gaspar de Carvajal, fray de la orden de Santo Domingo de Guzmán, que fue designado a acompañar los expedicionarios y registrar todos los acontecimientos y hechos importantes en el viaje sobre el descubrimiento de la Amazonía, como la presencia del mito de las mujeres guerreras, lasAmazonas, que acompañaba a los conquistadores desde los primeros viajes en busca de nuevos mundos.
SOSTENIBILIDAD Y CADENAS AGROPRODUCTIVAS DE CACAO EN EL PERÚ PERSPECTIVAS DESDE LAS REGIONES PIURA Y SAN MARTÍN
Authors: Acero Lourdes Amparo Lares 
Published: June 2020

La sostenibilidad de la cadena de valor de cacao en el Perú resulta importante, porque se encuentra relacionada directamente con el crecimiento económico de las familias productoras de cacao y pueden tener la posibilidad de salir de la pobreza. Los factores identificados en las cooperativas APPROCAP en Piura y ACOPAGRO en San Martín, que favorecen la sostenibilidad de esta cadena, son la diversidad de material nativo y su alta calidad, respectivamente. Ambas características son reconocidas en mercados especializados que pagan mejores precios por estos cacaos, lo que ha favorecido además su producción continua. El desarrollo de capacidades a los socios productores, así como la asociatividad son también factores importantes en ambas cadenas que han favorecido su sostenibilidad. Las políticas nacionales sobre cacao en el Perú deben poner más atención a sus diferentes cacaos a pesar de que muchos de ellos, los nativos, tengan menos volumen productivo pero mucho valor de biodiversidad y a la vez económico.
Analisis Kuat Tarik dan Elongasi Film Gelatin – Khitosan
Authors: Wafi Abdul , Atmaja Lukman , Ni’mah Yatim Lailun 
Published: June 2020

<p>Gelatin and chitosan have been widely used in various industries such as pharmaceuticals, textiles, cosmetics, food processings, and others. Gelatin has different mechanical properties compared to chitosan, especially its tensile strength and elongation. In this study, gelatin (Ge) was obtained commercially, while chitosan (Ch) was obtained from the skin of windu shrimp via deproteination, demineralization, and deacetylation of chitin into chitosan. The results of this study indicated that the degree of deacetylation of chitosan was 52.06%. Afterward, various films including Ge, Ch, Ge 4%-Ch 1.5%, Ge 4%-Ch 3%, Ge 4%-Ch 4% were prepared by the casting method. The film of Ge 4%-Ch 4% exhibited the best tensile strength and elongation with values of 0.6 MPa and 21.53%, respectively.</p><p> </p><p>Gelatin dan kitosan merupakan biopolimer alam yang selama ini banyak digunakan di berbagai industri seperti farmasi, tekstil, kosmetik, pengolahan pangan dan lain sebagainya. Gelatin memiliki sifat mekanik yang berbeda dibandingkan dengan kitosan terutama kuat tarik dan elongasinya. Pada penelitian ini, gelatin (Ge) diperoleh secara komersial, sedangkan kitosan (Ch) diperoleh dari kulit udang windu melalui proses deproteinasi, demineralisasi, dan deasetilasi kitin menjadi kitosan. Hasil penelitian ini menunjukkan derajat deasetilasi kitosan sebesar 52,06%. Selanjutnya, dilakukan proses pembuatan film Ge, Ch, Ge 4%-Ch 1,5%; Ge 4%-Ch 3%, Ge 4%-Ch 4% dengan menggunakan metode <em>casting</em>. Film Ge 4%-Ch 4% memiliki kuat tarik dan elongasi yang paling baik yaitu 0,6 MPa dan 21,53%, berturut-turut.</p><p> </p>
NTCP gene polymorphisms and Hepatitis B virus infection status in a Ghanaian population
Authors: Nyarko Eric , Obirikorang Christian , Owiredu W.K.B.A. , Asamoah Evans Adu , Acheampong Emmanuel , Aidoo Freeman , Ofori Emmanuel , Addy Bright Selorm , Asare-Anane Henry 
Published: June 2020

Abstract
Background: SLC10A1 gene codes NTCP, a receptor through which the hepatitis B virus (HBV) gets access into hepatocytes - a stage of the viral cycle necessary for replication. Polymorphism variants of SLC10A1 play roles in HBV infection, viral clearance, treatment outcome, and complications, in diverse ethnic groups and countries. However, no such study has been conducted in the Ghanaian population, a country with HBV endemicity. Therefore, an exploratory study was conducted to investigate the presence of three (3) single nucleotide polymorphisms (SNPs) in the SLC10A1 gene (rs2296651, rs61745930, and rs4646287) and assessed the risk of HBV infection among the Ghanaian population. Method: Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was used to determine the presence of the SNPs among 292 participants comprising 146 HBV infected persons as case-subjects and 146 HBV non-infected persons as control-subjects. Results: The minor allele frequency (T) of rs2296651 was present in a significantly high proportion of cases compared with the control group (11.6% vs. 3.1%, p<0.0001). The homozygote recessive variant of rs61745930 was present in 2.7% of the control group and 5.5% of the case group. Moreover, the minor allele frequencies of rs4646287 were 9.3% and 8.2% among the control and the case group, respectively (p =0.767). Under the dominant (CC) genetic model of inheritance, rs2296651 was found to be protective of HBV infection [OR= 0.18 (0.07-0.44)], whereas under the co-dominant and additive model, rs2296651 was a potential risk factor for HBV infection [OR= 5.2 (95%CI: 2.1 -12.8); 3.5 (95%CI: 1.6 -7.6], respectively. Variants of rs61745930 and rs4646287 were not associated with HBV infection (p > 0.05). Polymorphisms in SLC10A1, however, did not show any significant association with HBV infectivity (p >0.05). Conclusion: The study highlights some polymorphism proof that variants rs2296651, rs61745930, and rs4646287 exist in HBV-infected individuals in Ghana. Although variant rs2296651 was found to be associated with HBV infection, this association warrants more studies. Polymorphisms in SLC10A1 were not associated with HBV infectivity among the Ghanaian population. Further investigation is warranted to assess the offensive role of the relationship between rs2296651 and HBV infectivity.
Moderate rate of transmitted resistance mutations to antiretrovirals and genetic diversity in newly HIV-1 patients diagnosed in Benin.
Authors: TCHIAKPE EDMOND , Keke Rene K , Vidal Nicole , Ahoussinou Clément , Sekpe Olga , Dagba Hermione G , Gbaguidi Eric , Tonoukouen Conrad , Afangnihoun Aldric , Bachabi Moussa , Gangbo Flore A , Ndiaye Halimatou Diop , Kane Coumba Toure 
Published: June 2020

Abstract
Objective Seventeen years after the start of the IBAARV (Beninese initiative for access to antiretrovirals), transmitted drug resistance mutations in ARV-naïve patients and HIV-1 genetic diversity were investigated in Benin. Results Drug resistance mutations were detected in (27/248; 10.9%) according to the WHO SDRM 2009 list, with a predominance of mutations directed against NNRTIs drugs (24/248; 10%). Phylogenetic and recombination analyses showed a predominance of CRF02_AG strains (165/248; 66.5%) and a high genetic diversity with five other variants and 39 URFs (15.7%) which contained portions of strains that co-circulate in Benin. Eight recent transmission chains revealed active ongoing transmission of HIV-1 strains among ARV-naïve patients.Our study showed a moderate primary drug resistance mutations rate and also provided recent data on the HIV-1 variants that circulate in Benin. Regular monitoring of primary drug resistance is required to adapt HIV-1 treatment strategies and adoption of new WHO recommendations in Benin.
Electroacupuncture preconditioning alleviates myocardial injury through regulating mitochondrial function.
Authors: Wang Chunai , Liang Xi , Yu Yan , Li Yulan , Wen Xiaohui , Liu Min 
Published: June 2020

Abstract
Background Electroacupuncture is well known for its advantageous neuroanalgesic and therapeutic effects on myocardial ischemia-reperfusion injury. The purpose of the present research was to verify whether electroacupuncture can alleviate bupivacaine-induced myocardial injury. Methods Specific-pathogen-free Wistar rats were used to establish the bupivacaine-induced myocardial injury model. Western blot, PCR, transmission electron microscope and enzyme-linked immunosorbent (ELISA) methods were used to evaluate bupivacaine-induced structure injury and dysfunction of the mitochondria as well as the alleviating effects of lipid emulsion, acupoint injection, and electroacupuncture pre-treatment of the oxidase stress response. Results Bupivacaine caused structural damage, degradation, and swelling of mitochondria. Furthermore, it reduced adenosine triphosphate (ATP) synthesis and impaired energy metabolism in the mitochondria. Structural and functional impairment of the mitochondria was alleviated via lipid emulsion injection, acupoint injection, and electroacupuncture pre-treatment. Electroacupuncture pre-treatment of PC6 yielded a greater alleviating effect than others approaches. Following electroacupuncture pre-treatment of PC6 point, the number of mitochondria increased; apoptosis was reduced, enzymatic activity of cytochrome C oxidase (COX) and superoxide dismutase and expression of uncoupling protein 2, voltage-dependent anion channel 1, and Bcl 2 were upregulated and SLC25A6, MDA levels were downregulated. Additionally, our findings indicated that electroacupuncture pre-treatment of PC6 point exerted an effect on the mitochondria via the mitochondrial-transcription-factor-A / nuclear-respiratory-factor-1 / proliferator-activated-receptor-gamma-coactivator -1 pathway. Conclusion The present study revealed that electroacupuncture pre-treatment of PC6 could effectively alleviate bupivacaine-induced myocardial mitochondrial damage, thereby providing a theoretical basis for clinical studies and applications of this treatment method.
Whole exome sequencing identifies a novel mutation in spermine synthase gene (SMS) associated with Snyder-Robinson Syndrome
Authors: Qazi Talal J. , Wu Qiao , Aierken Ailikemu , Lu Daru , Bukhari Ihtisham , Hussain Hafiz M. J. , Yang Jingming , Mir Asif , Qing Hong 
Published: June 2020

Abstract
Background: Loss of function mutations in the spermine synthase gene (SMS) have been reported to cause a rare X-linked intellectual disability known as Snyder-Robinson Syndrome (SRS). Besides intellectual disability, SRS is also characterized by reduced bone density, osteoporosis and facial dysmorphism. SRS phenotypes evolve with age from childhood to adulthood. Methods: Whole exome sequencing was performed to know the causative gene/pathogenic variant. Later we confirmed the pathogenic variant through Sanger sequencing. Furthermore, we also performed the mutational analysis through HOPE SERVER and SWISS-MODEL. Also, radiographs were also obtained for affected individual to confirm the disease features. Results: In this article, we report the first Pakistani family consisting of three patients with SRS and a novel missense pathogenic variant in the SMS gene (c.905 C>T p.(Ser302Leu)). In addition to the typical phenotypes, one patient presented with early-onset seizures. Clinical features, genetic and in-silico analysis linked the affected patients of the family with Snyder-Robinson and suggest that this novel mutation affects the spermine synthase activityConclusion: A novel missense variant in the SMS, c.905C >T p. (Ser302Leu), causing Snyder- Robinson Syndrome (SRS) is reported in three members of Pakistani Family.
Variations In Trim5α And Cyclophilin A Genes Among HIV-1 Elite Controllers and Non Controllers In Uganda; A Laboratory-Based Cross-sectional study
Authors: Amanya Sharon Bright , Nyiro Brian , Waswa Francis , Obura Bonniface , Nakaziba Rebecca , Nabulime Eva , Katabazi Ashaba Fred , Nabatanzi Rose , Bayiyana Alice , Mboowa Gerald , Kayongo Alex , Wayengera Misaki , Sande Obondo J. 
Published: June 2020

Abstract
Background Tripartite Motif Containing 5 alpha (TRIM5α), a restriction factor produced ubiquitously in cells and tissues of the body plays an important role in the immune response against HIV. TRIM5α targets the HIV capsid for proteosomal destruction. Cyclophilin A, an intracellular protein has also been reported to influence HIV infectivity in a cell-specific manner. Accordingly, variations in TRIM5α and Cyclophilin A genes have been documented to influence HIV-1 disease progression. However, these variations have not been documented among Elite controllers in Uganda and whether they play a role in viral suppression remains largely undocumented. Our study focused on identifying the variations in TRIM5α and Cyclophilin A genes among HIV-1 Elite controllers and non-controllers in Uganda.Results From the sequence analysis, the rs10838525 G>A mutation in exon 2 of TRIM5α was only found among elite controllers (30%) while the rs3824949 in the 5’UTR was seen among 25% of the non-controllers. In the Cyclophilin A promoter, rs6850 was seen among 62.5% of the non-controllers and only among 10% elite controllers. Furthermore, rs17860048 in the Cyclophillin A promoter was predominantly seen among elite controllers (30%) and 12.5% non-controllers. From gene expression analysis, we noted that the respective genes were generally elevated among elite controllers, however, this difference was not statistically significant (TRIM5α p=0.6095; Cyclophilin A p=0.6389). Conclusion Variations in TRIM5α and Cyclophillin A promoter may influence HIV viral suppression. The rs10838525 SNP in TRIM5α may contribute to viral suppression among HIV-1 elite controllers. The rs6850 in the cyclophillin A gene may be responsible for HIV-1 rapid progression among HIV-1 non-controllers. These SNPs should be investigated mechanistically to determine their precise role in HIV-1 viral suppression.
Isolation, Identification and Antimicrobial Susceptibility Tests of E. Coli and Salmonella from Diarrheic Calves in and Around Sebeta Town
Authors: Merera Olana , Regassa Atnafu , Gizaw Fikru , Adugna Biyansa , Abera Shubisa 
Published: June 2020

Abstract
Background

Calves are important assets for replacement of cows for the future dairy and beef herd sustainability. However, calf hood diseases have a significant financial impact on dairies resulting from treatment costs, genetic loss, and impaired future performance.
Objective

The purpose of the present research is for isolation and identification of Salmonella and E. coli infection in diarrheic calves, assessing risk factors for occurrence of calf diarrhea and examining antimicrobial susceptibility of isolated bacteria’s.
Methodology:

A cross- sectional study on the isolation, identification and antimicrobial susceptibility of E. coli and Salmonella was carried out on calves up to two months of age from November, 2018 to March, 2019 in and around Sebeta town, Ethiopia. The detection of E. coli and Salmonella was done by conventional bacteriological method and biolog (semiautomatic machine). The data was analyzed by Stata 12.
Results

It was found that the detection rate of E. coli and Salmonella from 129 sampled diarrheic calves were 65 (50.3%) and 8 (6.2%) respectively. Age (E. coli, X2 = 9.429, P = 0.024; Salmonella, X2 = 8.161, P = 0.043), sex (E. coli, X2 = 11.225, P = 0.001, though not significant for Salmonella) and feeding time of colostrum (X2 = 7.510, P = 0.023; Salmonella, X2 = 6.678, P = 0.036) were risk factors associated with the incidence of E. coli and Salmonella. The antimicrobial study revealed that most of the E. coli was highly susceptible to sulphamethoxazole˖trimethoprim, chloramphenicol, gentamicin and ampicillin and highly resistant to tetracycline (29.23%) and followed by polymixin B (6.1%), and from isolates of Salmonella all showed 100% susceptibility for sulphamethoxazole˖trimethoprim and polymixin B, 87% to Ampicillin, chloramphenicol and tetracycline while 75% to gentamicin.
Conclusion

Based on those findings, it can be concluded that calf diarrhea was found to be high and could affect dairy production in the study area. The triggering ecological, social and bacterial genetic factors for the widespread distribution of multidrug resistant Salmonella and E.coli are not known, thus requires comprehensive study.
Does frailty predict postoperative outcomes in geriatric patients receiving surgery for colorectal cancer? A systematic review and meta-analysis
Authors: YANG XIAOBO , Xu Chenying , Cao Jiumei , Xie Li , Qiu Qingqing , Hu Jiaan , Yu Lifen , Woo Jean 
Published: June 2020

Abstract
Background

Surgery remains the mainstay of colorectal cancer (CRC) and substantially reduces cancer-related morbidity and mortality. Preoperative assessment for frailty in geriatric patients is critically important in risk stratification and clinical decision-making. In this systematic review and meta-analysis, we aimed to quantitatively summarise the effect of frailty on postoperative outcomes in geriatric patients receiving surgery for CRC.

Methods

A systematic literature search was conducted in MEDLINE, Cochrane and EMBASE from inception to 30 April 2020. Fully published articles reporting risk estimate(s) of frailty on postoperative complication(s), readmission and/ or mortality in patients aged ≥65 years who received surgery for CRC were eligible for qualitative and quantitative analyses.

Results

Across 10 articles of 9 unique studies (n = 69332) that were eventually included in the systematic review and meta-analysis, overall prevalence of frailty was 23.0% (95% CI: 11–43%, I 2 = 100%). Odds ratios (ORs) on overall and severe postoperative complications were respectively increased by 2.36- (95% CI: 1.66–3.35, P <0.01; I 2 = 12%) and 2.35-fold (95% CI: 1.30–4.27, P <0.01; I 2 = 72%) in frail patients compared to non-frail counterparts. On pooled analysis, frailty was significantly associated with an increased risk of postoperative readmission (OR:1.91; 95% CI: 1.35–2.70, P <0.01; I 2 = 6%). Whilst a significantly higher risk of frailty on mortality during 12 months after CRC surgery was observed (OR: 5.52; 95% CI:4.40–6.92, P <0.01; I 2 = 89%), the summary OR on 30-day/ inpatient mortality crossed the null line (OR: 1.65; 95% CI: 0.56–4.93, P = 0.37; I 2 = 55%). Funnel plot and Duval-Tweedie’s trim and fill test did not reveal significant publication bias.

Conclusions

In the studies reviewed, frailty appeared to be associated with increased risks for postoperative complications, readmission and mortality during 12 months in patients aged ≥65 years who received surgery for CRC. Nevertheless, no significant association between frailty and 30-day/ inpatient postoperative mortality was observed.
Who suffers from pharmaceutical poverty and what are their needs? Evidence from a Spanish region.
Authors: Rubio-Valera Maria , Marqués-Ercilla Silvia , Peñarrubia-Maria Maria Teresa , Urbanos-Garrido Rosa , Borrell Carme , Bosch Jordi , Sánchez-Viñas Alba , Aznar-Lou Ignacio 
Published: June 2020

Abstract
Background

Pharmaceutical poverty occurs when a patient cannot afford the cost of prescribed medication and/or medical products. Non-profit organisations are covering the cost of medication to those patients in some contexts. The aim of the study was to describe the population of beneficiaries of the PB, a non-governmental organization based on Primary Healthcare system, which provides free of charge access to medicines, and their utilization pattern of medicines and healthcare products.
Methods

This was an observational study using PB beneficiary data collected between November 2017 and December 2018 in Catalonia. The Catalan Health Service provided information from the general population. A descriptive analysis of the beneficiaries’ characteristics was conducted and compared to general population.
Results

The beneficiaries (N = 1206) were mainly adults with a low level of education, unemployed, with functional disability and with ≥ 1 children. Compared with the general population, the beneficiaries were older, had a lower level of education, showed a higher prevalence of functional disability, were less likely to be Spanish, and more likely to be divorced and unemployed. The beneficiaries were polymedicated and most were using medication related to the nervous (79%), musculo-skeletal (68%) and cardiovascular system (56%) and alimentary tract and metabolism (68%). Almost 19% of beneficiaries used healthcare products. Female beneficiaries were older and more likely to be divorced or widowed, employed and with children. Compared to men, women were more likely to use medicines for pain and mental disorders. The paediatric group used medications for severe, chronic conditions (heart diseases, autoimmune diseases, conduct disorders and attention deficit hyperactivity disorder).
Conclusions

Patients with severe, chronic and disabling conditions are affected by pharmaceutical poverty. While the system of co-payment remains unchanged, family physicians and paediatricians should explore economic barriers to treatment and direct their patients to resources that help to cover the cost of treatment.
Zimbabwe’s Indigenous SME Policy Framework, A Tool for Black Empowerment
Authors: Musabayana Godfrey Tambudzayi , Mutambara Emmanuel 
Published: June 2020

Abstract
Zimbabwe embarked on the policy to empower its local people through enacting into law a policy that targeted the empowerment of its citizens. The ‘Indigenisation and Empowerment Act’ (IEP) of Zimbabwe was made into law when the state President, Robert Mugabe signed the Act on March 7, 2008. It is stated in the (IEP) that the foreign-owned companies should offer at least 51 percent of their shares to indigenous Zimbabweans, and also provide for the creation of an empowerment fund to finance the acquisition of working capital, shares, and other forms of finance for indigenous people. The indigenisation process mainly focus on Small and Medium Enterprises (SMEs) as the key drivers of the new economic dispensation hence their performance is of great significance to this cause. This development was viewed by the citizens as the best strategy to implement in order to empower the nation to participate in the economic development of the country. Methodologically, the study critically analysed the literature of indigenization policies that were applied by some regions of the world and how they impacted on the disadvantaged groups. The researcher went on to analyse the Zimbabwean indigenous policies and how they impacted the SMEs and drew insights from the findings of other researchers.