bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identifification of Key Genes Involved in Colorectal Cancer: A Bioinformatics Analysis
Authors: Lu Dongxue , Yan Jing , Sun Zhiguang , Li Xuan , Liu Feng , Zou Kun , Ding Pinpin , Liu Haixia , Zhu Li 
Published: October 2020

Abstract
BackgroundColorectal cancer is the life-threatening tumor with both high prevalence and mortality worldwide. However, the molecular mechanism behind it remains unknown. Methods: Herein, the potential prognostic candidate biomarkers for colorectal cancer were tested by Bioinformatical analysis combined with the CRC clinical samples. Three data sets (GSE32323, GSE44076 and GSE43078) were collected from the gene expression omnibus (GEO). The limma and clusterProfifiler packages were used to identify differentially expressed genes (DEGs) and conduct functional enrichment analysis, respectively. To retrieve Interacting Genes (STRING) database, protein–protein interaction (PPI) network was built up using Search Tool, and Cytoscape was applied to carry out the module analysis. Subsequently, the online tool GEPIA was employed to conduct overall survival analysis (http://gepia.cancer-pku.cn/index.html), and the Oncomine database was used to analyze prognostic candidate biomarkers. Finally, 4 key hub genes were selected for validation of their expression levels in 9 patients newly diagnosed with CRC via reverse transcription‑quantitative PCR (RT‑qPCR). To evaluate the accuracy of prediction, time-dependent receiver operating characteristic (ROC) was applied.ResultsIn total, 547 DEGs got identifified, inclusive of 475 downregulated and 72 upregulated genes with a signifificant enrichment in the cellular response to hypoxia, the positive control of ERK1 and ERK2 cascade and the positive control of apoptotic process. The enhanced pathways were Pathways in cancer,PI3K-Akt signaling pathway,cGMP-PKG signaling pathway. Through the extraction of critical modules from the PPI network, 10 hub genes got removed. These 10 hub genes are all up-regulated genes and are highly expressed in colorectal cancer. Survival analysis shows that only CCNB1 and CCNA2 are associated with the survival prediction of colorectal cancer. Moreover, consistence is show between the TCGA data sets and the expression levels of the 4 hub genes. Receiver Operating Characteristic(ROC) curves showed that all CCNB1,CCNA2,AURKA and BUB1B have potential predictive value.Briely, new hub genes identifified can shed light on the underlying mechanism behind CRC carcinogenesis and development, which is coducive to detecting and treating CRC timely.
Total and High Density Lipoprotein Cholesterol Ratio is Associated With Metabolic Syndrome Among Very Elderly in Chengdu, China
Authors: Huang Gang , Xu Junbo , Deng Xiaoqi , Wu Jing , Xie Jiang , Cai Lin , Liu Hanxiong , Yu Xiuqiong , Zhang Tingjie 
Published: October 2020

Abstract
Backgroundmetabolic syndrome (MetS) is currently a major public health challenge worldwide. This study was to investigate the potential association between total and high density lipoprotein cholesterol ratio (THR) and MetS in very elderly population in Chengdu. Methodstotally 1056 very elderly (aged ≥ 80 years ) in Chengdu community were enrolled in this cross-sectional study. Geographic characteristics of participants were collected and laboratory measurement were performed. Metabolic syndrome (MetS) was defined according to Chinese and international diabetes federation (IDF) criteria respectively. Logistic analysis was used to investigate the potential association between THR and MetS. The receiver operating characteristic curve (ROC) analysis was used to evaluate the efficiency of THR in predicting MetS. ResultsFinally 1038 participants were included in statistical analysis. The mean age was 83.6±3.4 years and 52.6% was men and 21.6% of which suffered from MetS. Participants with MetS had relatively higher waist circumference, body weight, blood pressure, fast plasma glucose, non-high density lipoprotein cholesterol and THR. The logistic analysis revealed that THR was associated with MetS according to both Chinese (odds ratio(OR): 3.053, 95% confidence interval(CI): 2.464-3.782, P<0.001) and IDF criteria (OR: 2.458, 95%CI: 2.016-2.995, P<0.001). And the ROC analysis found that the area under curve of THR was 0.800 (95%CI: 0.749-0.852, P<0.001) and 0.727 (95%CI: 0.669-0.786, P<0.001) for predicting MetS according to Chinese and IDF criteria, respectively.ConclusionsTHR is associated with MetS in this community very elderly population in Chengdu.
Common Microbial Agents in Asthmatic Children With Respiratory Infection and Productive Cough
Authors: Sabet Faezeh , Mirhosseini Ali , Basharkhah Samira , Mohereri Mehran , Mohammadi Fatemeh Sadat , Ahanchian Hamid , Rezaee SA Rahim 
Published: October 2020

Abstract
BackgroundMicrobes can influence on the development and somehow on triggering of asthma attacks. Therefore, in this study the more significant microbial infections which trigger the attacks in children were evaluated.MethodsA total of 41 nasopharyngeal and oro-pharyengeal swabs were obtained from the Pediatric Allergy Clinic of two educational hospitals, and sent to a Molecular Laboratory for evaluation of 21 bacterial and viral respiratory pathogens using qPCR-TaqMan method.ResultsThe main bacterial infections were S.aur 18/41 (43.9%), S.pneu 16/41 (39%), C.pneu 12 /41 (29.3%), and HIB 17/41 (41.5%) while the most viral infections were and HRSV 3/41 (7.3%) and FluB, HRV, HMPVA.B, HPIV-2,3,4, HcoV-63 and HcoV-229 in 2 cases (4,9%), in asthmatic children. Although, bacterial infections were more common in both gender, the frequency of those agents were statistically difference between girls and boys population (P=0.02). There were positive correlations between S. pneu infection with asthma attack and bronchitis (P= 0.02 and P= 0.001, respectively). Furthermore, a positive correlation was found between AV and RSVA.B infections with allergic rhinitis (P= 0.02 and P= 0.001, respectively).ConclusionIn conclusion, it is more likely that in this region with a population of 6.2 million and more than 25 million tourists, the bacterial respiratory infections, particularly, HIB, S.aur and S. pneu were more common, however, and consistence with other studies HRSV and Flu.B have been dominant viral infections in asthmatic attacks.
Right Coronary Artery May Grow Even Better than Normal After Arterial Switch Operation for Complete Transposition of the Great Arteries
Authors: Machida Daisuke , Isomatsu Yukihisa , Goda Motohiko , Suzuki Shinichi , Kasama Keiichiro , Minami Tomoyuki , Yasuda Shota , Choh Tomoki , Goda Masami , Tominaga Norihisa , Kaneko Shotaro , Masuda Munetaka 
Published: October 2020

Abstract
Background: It is unclear if coronary arteries properly grow in patients who underwent arterial switch operation for complete transposition of the great arteries. The purpose of this study was to clarify the mode of coronary growth and size in these patients. Methods: Eighteen patients who underwent arterial switch operation for complete transposition of the great arteries from 2000 to 2012 in our institution, and in whom coronary angiography was performed in late operative phase, were enrolled in this study. Growth of coronary arteries was evaluated by cubage of coronary arteries based on analyses with coronary angiography. Coronary arteries were divided into small segments and each segment was approximated by a truncated right circular cone. The sum of the cubage of each truncated cone in one coronary artery was approximated as total cubage of the coronary. the coronary cubage index was then calculated by dividing total cubage of a coronary artery by the patient’s body surface area. The coronary cubage indexes of the enrolled patients were compared with that of control patients with healed Kawasaki disease.Results: The left coronary cubage indexes of the complete transposition of the great arteries group and the control group were 1.05 ± 0.34 and 0.94 ± 0.34 (p=0.598), respectively, and no significant deference was found between groups. On the contrary, the right cubage index of the complete transposition of great arteries group was significantly larger than the control group (1.08 ± 0.44 and 0.54 ± 0.37, respectively; p=0.007), and total coronary cubage index (left coronary index + right coronary index) of the complete transposition of the great arteries group was also larger than the control group as well (2.13 ± 0.7 and 1.47 ± 0.6, respectively; p=0.026).Conclusion: The left coronary arteries after arterial switch operation for complete transposition of great arteries grow as large as normal; however, the right coronary arteries possibly grow even larger.
Modified Clavien-Dindo Classification to Analyze the Risk Factors of Early Complications after Radical Gastrectomy and the Impact of Complications on Long-Term Prognosis
Authors: Ma Jun , Wang Ying , Yu Shui-hong , Zhou Chao-pin , Wang Da-tian , Tang Dai-bin , Jiang Guoqin , Zhang Yaming 
Published: October 2020

Abstract
Background The modified Clavien-Dindo classification system was employed to investigate the occurrence of early complications along with the related risk factors following a radical gastrectomy procedure, with the view of conducting an analysis into the impact of complications on long-term prognosis. Methods The clinical data of 525 patients who had previously undergone a radical gastrectomy procedure for gastric cancer were analyzed in a retrospective fashion. Results Postoperative hospital stay: Complication group (17.88±8.472) days, severe complications group (23.10±7.594) days, significantly higher than non-complication group (10.26±1.973) days and non-severe complications group (11.47±4.712) days (P=0.000<0.05).Multivariate analysis: age (OR = 1.781, P = 0.013), preoperative comorbidity (OR = 1.765, P = 0.020), blood loss (OR = 2.153, P = 0.001), surgical approach (OR = 3.137, P = 0.000) were identified as an independent risk factor associated with early complications. Blood loss (OR=13.053, P=0.013), type of resection (OR=7.936, P=0.047) and nerve involvement (OR=3.670, P=0.009) were identified to be independent risk factors for severe complications.Severe postoperative complications (HR=1.595, P=0.107) and postoperative complications (HR=1.078, P=0.670) were not independent risk factors affecting the 5-year over survival rate. Conclusion Complications following radical gastrectomy were closely related to age, preoperative comorbidity, blood loss, and surgical approach; severe complications were closely related to blood loss, total gastrectomy, and nerve involvement; complications and severe complications were not found to be independent risk factors associated with long-term survival, that being said, they were significantly prolonged postoperative hospital stay.
Medical Electives in Sub-saharan Africa: A 14-years Student/NGO-driven Initiative
Authors: Quaglio Gianluca , Bortolozzo Marta , Benedetto Chiara Di , Lupato Alice , Cavagna Chiara , Putoto Giovanni 
Published: October 2020

Abstract
Background: This article sets out the medical experience on clinical electives in Sub-Saharan Africa driven by a collaboration between a student organisation and a Nongovernmental Organization (NGO). Preclinical medical students spent 4 weeks as part of a multidisciplinary medical team in Africa. Post-elective questionnaires were administered.Results: Of all, 141 students responded to the questionnaire. The participants came from 30 Italian universities. The main difficulties reported are due to the lack of resources for the exercise of the medical activity, and difficulties related to language and communication. The African experience had a positive impact on the progress of the studies upon return, with an increase in determination and motivation. The experience had also positive influences on the future professional choices and carriers. The experience seems to contribute not only to the professional growth, but also to the personal development.Conclusions: A well-structured, mentored experience in international health can have a positive impact on preclinical students’ attitudes, including their compassion, volunteerism, and interest in serving underserved populations. Italian medical schools should incorporate changes in their curricula to train socially responsible physicians.
CENPL, ISG20L2, LSM4&nbsp;and MRPL3&nbsp;Are Four Novel Hub Genes Involved in Breast Cancer Development and Progression
Authors: Yin Jinbao , Lin Chen , jiang Meng , Tang Xinbing , Xie Danlin , Chen Jingwen , Ke Rongqin 
Published: October 2020

Abstract
BackgroundAs a highly prevalent tumor disease worldwide, Further elucidation of the molecular mechanisms of the occurrence, development and prognosis of breast cancer remain an urgent need. Identifying hub genes involved in these pathogenesis and progression can potentially help to unveil its mechanism and provide novel diagnostic and prognostic markers for breast cancer.MethodsIn this study, we systematically integrated multiple bioinformatic methods, including robust rank aggregation (RRA), functional enrichment analysis, protein-protein interaction (PPI) networks construction and analysis, weighted gene co-expression network analysis (WGCNA), ROC and Kaplan-Meier analyses, DNA methylation analyses and genomic mutation analyses, GSEA and GSVA, based on ten mRNA datasets to identify and investigate novel hub genes involved in breast cancer. In parallel, RNA in situ detection technology was applied to validate those novel hub gene.ResultsEZH2 was recognized as a key gene by PPI network analysis. CENPL, ISG20L2, LSM4 and MRPL3 were identified as four novel hub genes through the WGCNA analysis and literate search. Among those five hub genes, many studies on EZH2 gene in breast cancer have been reported, but no studies are related to the roles of CENPL, ISG20L2, MRPL3 and LSM4 in breast cancer. These novel four hub genes were up-regulated in breast cancer tissues and associated with tumor progression. ROC and Kaplan-Meier indicated these four hub genes all showed good diagnostic performance and prognostic values for breast cancer. The preliminary analysis revealed those novel hub genes are four potentially candidate genes for further exploring the molecular mechanism of breast cancer.ConclusionWe identify four novel hub genes (CENPL, ISG20L2, MRPL3, and LSM4) that are likely playing key roles in the molecular mechanism of occurrence and development of breast cancer. Those hub genes are four potentially candidate genes served as promising candidate diagnostic biomarkers and prognosis predictors for breast cancer, and their exact functional mechanisms in breast cancer deserve further in-depth study.
Microarthropod population dynamics in a subtropical deciduous forest of the Eastern-Ghat hill range in India in response to variation in edaphic factors
Authors: Acharya Pratik , Samal Suryasikha , Mishra C.S.K. 
Published: October 2020

Abstract
Background: Soil microarthropods are considered as major groups of soil fauna which facilitate the decomposition of organics in soil. In forests, the sustenance of nutrient pool is dependent on the density and diversity of these animals. Edaphic factors of habitat play vital role in species distribution of any region. Any changes in population structure of microarthropod may affect the ecosystem adversely. This study reports the seasonal variation of microarthropod population of the orders Collembola, Acari and Hymenoptera in five sampling zones, degraded (DF), dense mixed (DMF), open mixed (OMF), bamboo (BF) and wet land (WL) in a subtropical deciduous forest (Chandaka-Dampara) of Eastern India. Results: Seven species of Collembola and four species each of Acari and Hymenoptera were identified. Ecological indices did not show noticeable species diversity in different sampling zones of the forest. Heatmap analysis indicated high relative abundance of Collembola in WL irrespective of season. The abundance of Acari was high in OMF and DF, Hymenoptera in DMF and OMF for dry and wet season respectively. Wet season indicated significantly higher microarthropod population irrespective of species. The correlation colour matrix and principal component analysis (PCA) showed significant positive correlation of arthropod population with soil moisture and organic carbon. Significant population variation in the animal population were observed between dry and wet seasons. Conclusion: The forest floor was dominated by Collembola order of microarthropod species irrespective of sampling zone and season. Soil moisture and carbon contents in different seasons were found to be most sensitive growth regulators of microarthropod populations In Chandaka forest of Eastern India.
Epidemiological aspects of tuberculosis in HIV infected children
Authors: Vasilieva E. B. , Lozovskaya M. E. , Klochkova L. V. , Yarovaya Yu. A. , Noskova O. M. 
Published: October 2020

The objective: to assess the methods and time of detection of tuberculosis and HIV infection in children with the co-infection, epidemiological risk factors to develop tuberculosis and effectiveness of preventive measures.Subjects and methods. 75 children in the age from 0 to 14 years old were enrolled in the study. The TB/HIV Group included 25 children with TB/HIV co-infection. The TB Group included 50 HIV negative children with tuberculosis, they made a comparison group.Results: out of 25 children with TB/HIV, only 18 (72%) were aware of perinatal exposure to HIV. HIV infection was confirmed in 11/25 (44%) children during the first months of life, in the remaining 14/25 (56%) it was confirmed later, the latest at 13 years old. In 4 children, HIV infection was detected during examination for tuberculosis. In the TB/HIV Group, in 14/25 (56%) children were exposed to tuberculosis in their families as well as 35/50 (70%) in the TB Group. The positive result of a sputum test of the index case was registered in 7/14 (50%) children of the TB/HIV Group and 19/35 (54.2%) children of the TB Group. Of 25 children of the TB/HIV Group, 17 children (68%) were vaccinated with BCG, and in 9 of them the vaccination was not done properly.
Clinical and economic aspects of enhanced treatment efficacy of multiple drug resistant tuberculosis
Authors: Markelov Yu. M. , Lesonen A. S. 
Published: October 2020

The objective of the study: To evaluate the clinical and economic efficacy of chemotherapy in patients with newly diagnosed multiple drug resistant tuberculosis (MDR).Subjects and methods. The number and structure of MDR tuberculosis patients living on the territory of the Republic of Karelia from 2008 to 2017 were studied. The clinical and economic efficacy of treatment of MDR tuberculosis patients was assessed.Results. In 2011-2017, decrease in the number of MDR patients in the Republic of Karelia was mainly due to enhanced treatment efficacy of new MDR tuberculosis cases. The introduction of social support methods (food parsels) and express drug sensitivity testing to rifampicin using the Gene Xpert MTB/RIF was associated with higher treatment efficacy – from 37.2% (2013) to 58% (2014), the lower rate of treatment defaults, and significant decrease in mortality, as well as reduction of costs for curing of one MDR tuberculosis patient from1.68 million rubles in 2012 up to 0.77 million rubles in 2014.
Xanthosoma sagittifolium is resistant to Meloidogyne spp. and controls Meloidogyne enterolobii by soil biofumigation
Authors: Gomes Vanessa Alves , de Jesus Silva Fabíola , Baquião Eunice Maria , Faria Luana Viana , Ferreira Júlio César Antunes , Pedroso Marcio Pozzobon , Broetto Fernando , Wilcken Silvia Renata Siciliano 
Published: October 2020

Abstract

Meloidogyne is a relevant plant-parasitic nematode that causes enormous damage. It is very challenging to control, and there are not many chemicals available on the market for that. As an alternative method of nematode control, biofumigation is increasingly gaining space. This research aimed to study the reaction of Xanthosoma sagittifolium to Meloidogyne enterolobii, M. incognita, and M. javanica and soil biofumigation with X. sagittifolium leaves for M. enterolobii control. The reaction test was performed in the populations 0 (control), 333, 999, 3,000, 9,000, 27,000 eggs and eventual juveniles. X. sagittifolium did not host the Meloidogyne species studied, even in a high population. X. sagittifolium leaves incorporated in soil at concentrations 0 (control), 0.45, 0.9, 1.8, 3.6 g were also studied to control M. enterolobii, and they were able to reduce galls and eggs. The number of galls and egg masses was reduced to a concentration of 1.8 g. In the maximum concentration, the number of galls was less than 15 galls, and the eggs were also reduced to less than 200 eggs. As these macerates emitted nematicidal volatile organic compounds (VOCs) against M. enterolobii, it reduced the infectivity and reproduction of nematodes.
DEPRESSÃO E ANSIEDADE NA ENFERMAGEM EM UNIDADE DE TERAPIA INTENSIVA
Authors: Bhenson Tavares Barbosa Malom , Barbosa de Lima Nascimento Daniela , Larissa Nepomuceno Torres Rebeca , Patrícia Pereira de Moraes Carla , Cristiane Soares da Silva Erika , Windysa de Sousa Silva Manayra , Gomes de Melo Michel 
Published: October 2020

Introdução: A depressão é definida como uma síndrome, composta de diversos sintomas físicos e emocionais, com implicações sobre as capacidades dos indivíduos em suas diversas esferas. Numa UTI, é quesito que o profissional possua agilidade na tomada de decisões, um cuidado livre de danos, conhecimento e controle emocional. Esses elementos, agregados aos hábitos de vida, conflitos e estresse no ambiente laboral podem desencadear nesses profissionais transtornos como depressão e ansiedade. Objetivo: Identificar a prevalência de depressão e ansiedade em profissionais de enfermagem que atuam em Unidade de terapia intensiva adulto. Metodologia: Pesquisa descritiva, transversal com abordagem quantitativa. Por tratar-se de um censo, a população foi composta por toda equipe de enfermagem que atua em Unidade de terapia intensiva Adulto e que atendiam aos critérios de elegibilidade. Para a coleta de dados utilizou-se um questionário sociodemográfico e os inventários de depressão e ansiedade de Beck. Adotou-se estatística descritiva para a análise dos dados. Resultados: A média de idade dos profissionais foi de 35 anos, onde 86% são do gênero feminino e sua maioria são casados (46%). Observou-se que 77% da amostra não apresenta sintomas depressivos. Já no que se refere aos sintomas ansiosos, 85% apresentaram grau mínimo de ansiedade. Conclusões: Os resultados desse estudo evidenciaram uma baixa prevalência de depressão e ansiedade. Tais resultados trazem aos gestores de saúde a possibilidade de atentar-se e rever as práticas adotadas nas instituições hospitalares. Recomenda-se que outros estudos, com análises mais robustas, sejam realizados no intuito de se ampliar o conhecimento sobre a temática.
Palavras-Chave: Depressão; Ansiedade; Saúde mental; Equipe de enfermagem; Unidades de Terapia Intensiva.