bluuebunny/crossref_metadata_2025_split huggingface dataset:
Determination of PAHs, PCBs, Minerals, Trace Elements, and Fatty Acids in Rapana thomasiana as an Indication of Pollution
Authors: Namiesnik Jacek , Szefer Piotr , Sliwka-Kaszynska Magdalena , Moncheva Snejana , Arancibia-Avila Patricia , Toledo Fernando , Ham Kyung-Sik , Kang Seong-Gook , Gorinstein Shela 
Published: February 2020

Abstract
The aim of this investigation was to introduce several analytical methods for determination of polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs), minerals, trace elements, and fatty acids in Rapana thomasiana as a marine pollution indicator organism. The chemical differences of the gastropod Ra. thomasiana from polluted and nonpolluted sites of the Black Sea on the Bulgarian coast were investigated. Chromatography and high-resolution inductively coupled plasma/MS analyses were used for evaluation of PAHs, PCBs, fatty acids, minerals, and trace elements. These methods can be applied to other marine products.
Quantitative Analysis of Closantel and Rafoxanide in Bovine and Ovine Muscles by High-Performance Liquid Chromatography with Fluorescence Detection
Authors: Yeung Hoi-Sze , Ching Wing-Hong , Lai Shirley Sau-Ling , Lee Wai-On , Wong Yiu-Tung 
Published: February 2020

Abstract
An HPLC method with a fluorescence detector (HPLC-FLD) was described for the quantitative determination of closantel and rafoxanide in bovine and ovine muscles. A structural analog closely related to rafoxanide, viz., N-[4-(4-chlorophenoxy) phenyl]-2-hydroxy-3,5-diiodobenzamide, was synthesized as an internal standard. Bovine and ovine muscles were extracted with acetonitrileacetone (60 + 40, v/v) followed by cleanup on mixed mode anionic exchange SPE cartridges. After evaporation and reconstitution with the mobile phase, the sample was analyzed by HPLC-FLD using internal standard calibration. The method was validated by using fortified bovine and ovine muscles at 15, 30, and 60 µg/kg. The accuracy and RSD were 70110% and 10%, respectively.
Determination of Trace Amounts of Pd(II) Ions in Water and Road Dust Samples by Flame Atomic Absorption Spectrometry After Preconcentration on Modified Organo Nanoclay
Authors: Afzali Daryoush , Mostafavi Ali , Afzali Zahra 
Published: February 2020

Abstract
This paper describes the application of organo nanoclay, an easily prepared and stable solid sorbent, to the preconcentration of trace amounts of palladium ions in aqueous solution. The organo nanoclay was prepared by adding tetradecyldimethylbenzylamonium chloride onto montmorillonite, which was then modified with 1-(2-pyridylazo)-2-naphthol. The modified nanoclay was used as a solid sorbent for separation and preconcentration of trace amounts of Pd(II) ions, and a simple, sensitive, and economical method was developed for determination of trace amounts of palladium by flame atomic absorption spectrometry. The sorption of Pd(II) ions was quantitative in the pH range of 1.55.0, whereas quantitative desorption occurred with 5.0 mL of a mixture containing 1.0 M thiourea and 1.0 M HCl. The RSD of the method was 2.1 (n = 10; concn = 0.5 g/mL), and the LOD (3bl; = SD and bl = blank) was 0.1 ng/mL. The calibration curve was linear for concentrations of 0.58.0 g/mL in the initial solution, and the preconcentration factor was 140. The maximum capacity of the sorbent was 2.4 mg Pd(II)/g modified organo nanoclay. The influences of the experimental parameters, including sample pH, eluant volume, eluant type, sample volume, and interfering ions, on the recoveries of the palladium ion were investigated. The proposed method was applied to the preconcentration and determination of palladium in different samples.
A Fast, Inexpensive, and Safe Method for Residue Analysis of Meptyldinocap in Different Fruits by Liquid Chromatography/Tandem Mass Spectrometry
Authors: Banerjee Kaushik , Dasgupta Soma , Jadhav Manjusha R , Naik Dattatraya G , Ligon Axel Patrick , Oulkar Dasharath P , Savant Rahul H , Adsule Pandurang G 
Published: February 2020

Abstract
An analytical method is reported for residue analysis of the fungicide meptyldinocap in different fruit matrixes that involves extraction with ethyl acetate, hydrolysis of the residues with ethanolamine, and determination by LC/MS/MS. The method involves extraction of 10 g sample with 10 mL ethyl acetate; evaporation of the ethyl acetate phase to dryness, and subsequent hydrolysis of the residues to 4,6-dinitro-2-(1-methylheptyl) phenol on reaction with 1 ethanolamine. The pH of this hydrolyzed product was neutralized with formic acid and analyzed by LC/MS/MS. The hydrolysis reaction followed pseudo-first-order kinetics, and the reaction product was spectroscopically confirmed as 2-(1-methylheptyl)-4,6-dinitrophenol. The method offered &gt;80 recoveries at an LOQ of 10 ng/g for grape and mango, 25 ng/g for pomegranate with intralaboratory Horwitz ratio &lt;0.5, and measurement uncertainties &lt;10 at LOQ levels. Considering first-order rate kinetics, activation energy, enthalpy of activation, and entropy of activation varied as solvent &gt; mango &gt; grape &gt; pomegranate. Free energy of activation at 298 K was higher than at 280 K and was similar for solvent and three matrixes at both temperatures.
Evaluation of VIDAS® Listeria species Xpress (LSX) Immunoassay Method for the Detection of Listeria species in Foods: Collaborative Study
Authors: Johnson Ronald L , Jechorek Robert P ,   Collaborators:, Andrews H , Bautista P , Bird P , Blamey S , Connell E , Cooper C , Cooper W D , Crowley E , Doane C , Elton S , Falkenberg R , Fernandes-Monteiro C , Gharst T , Gonzalez E , Hawes B , Hemming B , High E , Hsu D , Iannucci C , Kora L , Lara A , Lee M , Masanz G , Mattson D , Okolo C , Parra G , Ryan E , Torontali M , Vega H 
Published: February 2020

Abstract
In a multilaboratory study, the effectiveness of an alternative method for rapid screening of Listeria species compared to traditional reference methods was demonstrated in a variety of food products. A collaborative study was conducted to compare the VIDAS® Listeria species Xpress (LSX) method and the standard cultural methods for the detection of Listeria species in foods. Six food types were tested: vanilla ice cream, cheddar cheese, raw ground beef, frozen green beans, deli turkey, and cooked shrimp. Each food, inoculated with a different Listeria strain at two levels and uninoculated test portions, was analyzed by each method. A total of 15 laboratories representing government and industry participated. In this study 1134 tests were analyzed in the statistical analysis. There were 490 positives by the VIDAS LSX method using the sample boiling step, 483 positives by the VIDAS LSX method using the Heat and Go system, and 439 positives by the standard culture methods. Overall, the Chi-square result for the VIDAS LSX method with boiling for all foods was 7.25, indicating a significant statistical difference between the VIDAS method and the standard methods at the 5% confidence. For the VIDAS LSX method with the Heat and Go system, the Chi-square result for all foods was 5.37, indicating a significant statistical difference between the VIDAS LSX assay with the Heat and Go system and the standard methods at the 5% level of significance. In both cases, the VIDAS method was more sensitive than the standard methods. The LSX method detects Listeria species in foods with negative or presumptive positive results in a minimum of 30 h compared to at least 5 days for the cultural methods. Based on the results of this collaborative study, it is recommended that the VIDAS LSX method be adopted as an AOAC Official MethodSM for the detection of Listeria species in dairy products, vegetables, seafood, raw meats and poultry, and processed meats and poultry.
HPLC Analysis of Kaempherol and Quercetin Derivatives Isolated by Different Extraction Techniques from Plant Matrix
Authors: Skalicka-Woźniak Krystyna , Szypowski Janusz , Głowniak Kazimierz 
Published: February 2020

Abstract
Soxhlet extraction, ultrasound extraction, and accelerated solvent extraction (ASE), followed by RP-HPLC with a photodiode array detector was used for the determination of flavonoids in fruits of Peucedanum alsaticum. Three compounds were identified: a kaempherol derivative (astragalin), and two quercetin derivatives (quercitrin and hiperoside). The highest extraction yields of the selected compounds were obtained by use of exhaustive ASE.
A GC-ECD Method for Estimation of Free and Bound Amino Acids, γ-Aminobutyric Acid, Salicylic Acid, and Acetyl Salicylic Acid from Solanum lycopersicum (L.)
Authors: Meher Hari Charan , Gajbhiye Vijay T , Singh Ghanendra 
Published: February 2020

Abstract
A gas chromatograph with electron capture detection method for estimation of selected metabolites—amino acids (free and bound), γ-aminobutyric acid (GABA), salicylic acid (SA), and acetyl salicylic acid (ASA) from tomato—is reported. The method is based on nitrophenylation of the metabolites by 1-fluoro-2,4-dinitrobenzene under aqueous alkaline conditions to form dinitrophenyl derivatives. The derivatives were stable under the operating conditions of GC. Analysis of bound amino acids comprised perchloric acid precipitation of protein, alkylation (carboxymethylation) with iodoacetic acid, vapor-phase hydrolysis, and derivatization with 1-fluoro-2,4-dinitrobenzene in that order. The metabolites were resolved in 35 min, using a temperature-programmed run. The method is rapid, sensitive, and precise. It easily measured the typical amino acids (aspartate, asparagine, glutamate, glutamine, alanine, leucine, lysine, and phenylalanine) used for identification and quantification of a protein, resolved amino acids of the same mass (leucine and isoleucine), satisfactorily measured sulfur amino acids (methionine, cystine, and cysteine), and quantified GABA, SA, and ASA, as well. The developed method was validated for specificity, linearity, and precision. It has been applied and recommended for estimation of 25 metabolites from Solanum lycopersicum (L.).
Cloud-Point Extraction of Rhodamine 6G by Using Triton X-100 as the Non-Ionic Surfactant
Authors: Duran Celal , Ozdes Duygu , Bulut Volkan Numan , Tufekcī Mehmet , Soylak Mustafa 
Published: February 2020

Abstract
Cloud-point extraction (CPE) using the non-ionic surfactant Triton X-100 was used successfully to remove a highly toxic dye, rhodamine 6G (R6G), from water and wastewater. The effects of different analytical parameters such as pH, concentration of Triton X-100 (TX-100) and salts, equilibrium temperature, and incubation time on the efficiency of the extraction of R6G were studied in detail, and optimum conditions for dye extraction were obtained. Thermodynamic parameters including changes in Gibbs free energy, enthalpy, and entropy were also calculated, and these parameters indicated that the CPE of R6G was feasible, spontaneous, and endothermic in the temperature range of 75–95°C. The equilibrium solubilization capacity of TX-100 was found to be 1.10 mmol/mol by using Langmuir isotherm models. No significant interference effects were observed in the presence of phenol and its derivatives, some acidic and basic dyes and most of the anions and cations. It was concluded that the CPE process described in this paper can be an alternative technique for removal of dyes and other pollutants from waters and wastewaters.
Determination of Polyphenols in Mentha longifolia and M. piperita Field-Grown and In Vitro Plant Samples Using UPLC-TQ-MS
Authors: Krzyzanowska Justyna , Janda Bogdan , Pecio Lukasz , Stochmal Anna , Oleszek Wieslaw , Czubacka Anna , Przybys Marcin , Doroszewska Teresa 
Published: February 2020

Abstract
Nine polyphenols in the aerial parts of Mentha longifolia have been separated by chromatographic techniques. Their structures have been confirmed by HPLC/electrospray ionization-MS/MS. The compounds identified included rosmarinic acid, salvianolic acid L, dedihydro–salvianolic acid, luteolin–glucuronide, luteolin–diglucuronide, luteolin–glucopyranosyl–rhamnopyranoside, and eriodictyol–glucopyranosyl–rhamnopyranoside. The extracts of M. longifolia and M. piperita field plants, in vitro plants, callus tissues, and cell suspension cultures were profiled, and their polyphenol composition was compared in different tissues and quantified using ultra-performance column liquid chromatography (UPLC)/triple-quadrupole-MS in the selected-ion recording detection mode. Determination of desired compounds was based on calibration curves obtained for standards, which were previously isolated from M. longifolia aerial parts. The UPLC profiles revealed considerable differences in the synthesis of secondary metabolites among samples coming from field plants, in vitro plants, callus tissues, and cell suspension cultures. Plant tissues coming from field cultivation (for both M. piperita and M. longifolia) contained several phenolic compounds (flavonoids and phenolic acids), whereas plants from in vitro conditions, callus tissues, and suspension cultures contained only a few of them. Rosmarinic acid dominated in all of these samples. These results show that under in vitro conditions, the metabolism of phenolics undergoes a fundamental change.
ELISA Kit for Mustard Protein Determination: Interlaboratory Study
Authors: Cuhra Petr , Gabrovská Dana , Rysová Jana , Hanák Petr , Štumr Frantiek ,   Collaborators:, Baranda A , Barov S , Brychta J , Bulawov H , eni A , Dvorsk P , Guisantes Del Barco J A , Iametti S , Jimenez E , Karulnov L , Kubk M , Langerholc T , Laukkanen M-L , Malmheden Yman; I , Martnez de Maraon I , Martnez Quesada J , Pardo M A , Piknov L , Plicka J , Postigo Resa I , Suen Pardo; E , Takkinen K , Tomkov K 
Published: February 2020

Abstract
An interlaboratory study in 12 laboratories was performed to prove the validation of the ELISA method developed for the quantitative determination of mustard protein in foods. The ELISA kit used for this study is based on rabbit polyclonal antibody. This kit did not produce any false-positive results or cross-reactivity with in-house validation for a broad range of food matrixes with no detectable mustard protein. All participants obtained the Mustard ELISA kit with standard operational procedures, a list of samples, samples, and a protocol for recording test results. The study included 15 food samples and two spiked samples. Seven food matrix samples of zero mustard content and four samples with mustard declared as an ingredient showed mustard protein content lower than that of the first standard (0.42 mg/kg). Four samples with mustard declared as an ingredient revealed mustard protein content above 12.5 mg/kg (the highest standard). The statistical tests (Cochran, Dixon, and Mandel) and analysis of variance were used to evaluate the interlaboratory study results. Repeatability and reproducibility limits, as well as an LOQ (1.8 mg mustard proteins/kg) and LOD (0.5 mg mustard proteins/kg), for the kit were calculated.
Development and Validation of Micellar Liquid Chromatographic Methods for the Determination of Antibiotics in Different Matrixes
Authors: Rambla-Alegre Maria , Esteve-Romero Josep , Carda-Broch Samuel 
Published: February 2020

Abstract
Antibiotics are the most important bioactive and chemotherapeutic compounds to be produced by microbiological synthesis, and they have proved their worth in a variety of fields, such as medicinal chemistry, agriculture, and the food industry. Interest in antibiotics has grown in parallel with an increasingly high degree of productivity in the field of analytical applications. Therefore, it is necessary to develop chromatographic procedures capable of determining various drugs simultaneously in the shortest possible time. Micellar liquid chromatography (MLC) is an RP-HPLC technique that offers advantages over conventional HPLC as far as sample preparation, selectivity, and versatility are concerned. Its main advantage is that samples can be injected directly into the chromatographic system with no previous preparation step. This paper mainly focuses on the results of the authors' own recent research and reports the chromatographic conditions for determination of various antibiotics (penicillins, quinolones, and sulfonamides) in different matrixes (pharmaceuticals, biological fluids, and food). The work of other authors on MLC-based antibiotic determination has been included.
Rapid Microwave-Assisted Extraction and HPTLC- Photodensitometric Method for the Quality Assessment of Boerhaavia diffusa L.
Authors: Bhope Shrinivas G , Ghosh Vivek K , Kuber Vinod V , Patil Manohar J 
Published: February 2020

Abstract
A rapid and cost-effective method for the extraction of rotenoids in Boerhaavia diffusa L., based on the use of microwave-assisted extraction (MAE), is proposed. The conventional reflux, soxhlet, and maceration extraction methods were also conducted to validate the reliability of the new method. Under the optimized conditions, two rotenoids (boeravinone B and E) were extracted and quantified by HPTLC. The yield of boeravinone B and E achieved by MAE was 0.15 and 0.32% (w/w), respectively. The result showed that MAE-HPTLC is a simple, rapid, and solvent-sparing method for the extraction and quantitation of boeravinone B and E from B. diffusa L.
Dietary Supplement Laboratory Quality Assurance Program: The First Five Exercises
Authors: Phillips Melissa M , Rimmer Catherine A , Wood Laura J , Lippa Katrice A , Sharpless Katherine E , Duewer David L , Sander Lane C , Betz Joseph M 
Published: February 2020

Abstract
The National Institute of Standards and Technology (NIST) has established a Dietary Supplement Laboratory Quality Assurance Program (DSQAP) in collaboration with the National Institutes of Health Office of Dietary Supplements. Program participants measure concentrations of active and/or marker compounds as well as nutritional and toxic elements in food and dietary supplements distributed by NIST. Data are compiled at NIST, where they are analyzed for accuracy relative to reference values and concordance among the participants. Performance reports and certificates of completion are provided to participants, which can be used to demonstrate compliance with current Good Manufacturing Practices as promulgated by the U.S. Food and Drug Administration. The DSQAP has conducted five exercises to date, with total participation including more than 75 different laboratories and many more individual analysts.
Simultaneous Determination of Benzimidazoles and Their Metabolites in Plasma Using High-Performance Liquid Chromatography/Tandem Mass Spectrometry: Application to Pharmacokinetic Studies in Rabbits
Authors: Zhu Xinle , Wang Shuhuai , Qi Liu , Xu Qian , Zhang Congmin , Xu Shixin , Wang Xia , Li Dan , Hu Haiyan 
Published: February 2020

Abstract
An HPLC/MS/MS method was developed for the simultaneous determination of the following benzimidazole anthelmintics and metabolites in plasma: flubendazole, albendazole, fenbendazole, mebendazole, thiabendazole, hydrolyzed flubendazole, albendazole sulfoxide, albendazole sulfone, albendazole aminosulfone, oxfendazole, fenbendazole sulfone, aminomebendazole, hydroxymebendazole, and 5-hydroxythiabendazole. The sample preparation process involved a pH-dependent extraction of the analytes. Chromatographic separation was performed on a C18 column with a mobile phase gradient starting with methanol–water (20 + 80, v/v) containing 0.1% formic acid. The overall average recoveries of the analytes based on a matrix-matched calibration ranged from 75.0 to 120.0%, with RSD values of &lt;20.0%. The LODs ranged from 0.08 to 2.0 μg/kg and the LOQs from 0.3 to 5.0 μg/kg. The validated method was used in pharmacokinetic studies of benzimidazole compounds in rabbits, and the elimination of the metabolites was measured quantitatively.
Evaluation of TA10 Broth for Recovery of Heat- and Freeze-Injured Salmonella from Beef
Authors: -Horikoshi Naoko Kamisaki , Okada Yukio , Takeshita Kazuko , Sameshima Takashi , Kawasaki Susumu , Kawamoto Shinichi , Fratamico Pina M 
Published: February 2020

Abstract
The Bacteriological Analytical Manual (BAM) Salmonella pre-enrichment broth [lactose (LAC) broth], buffered peptone water, and universal pre-enrichment (UP) broth were compared with TA10 broth, developed in our laboratory, for recovery of heat- and freeze-injured Salmonella (55°C for 2–20 min and –20°C for 2 months, respectively) from beef. Beef samples were contaminated with single Salmonella serovars, and contamination levels of 0.44 to &lt;0.001 most probable number (MPN)/g and 0.74 to 0.14 MPN/g were used for heat- and freezing-induced injury studies, respectively. Twenty test portions (25 g) of the contaminated beef were pre-enriched in each broth, and the BAM Salmonella culture method was used thereafter. There was a significant difference (χ2 = 7.73) in recovery of heat-injured Salmonella between TA10 broth and LAC broth, 189 (67.5%) versus 156 (55.7%) positive samples, respectively, determined by plating onto selective agars and identification by biochemical tests. For the recovery of freeze-injured Salmonella, there was a significant difference (χ2 = 24.7) between TA10 and LAC broth, 189 (72.7%) versus 133 (51.2%) positive samples, respectively. TA10 broth was more effective than LAC broth and UP broth for recovery of freeze-injured Salmonella. The results indicate that TA10 broth should be used instead of LAC broth for testing of beef that may be contaminated with heat- and freeze-injured Salmonella spp.
An Efficient and Simultaneous Analysis of Caffeine and Paracetamol in Pharmaceutical Formulations Using TLC with a Fluorescence Plate Reader
Authors: Tavallali Hossein , Zareiyan J. S Faraneh , Naghian Maryam 
Published: February 2020

Abstract
A simple, rapid, and effcient method using TLC with a ﬂuorescence plate reader has been described for simultaneous determination of caffeine and paracetamol. Determination was carried out using the ﬂuorescencequenching action of caffeine and paracetamol on a TLC plate with a ﬂuorescent indicator at λ ex = 254 nm in the linear ranges of 0.2–1.9 and 0.03–1.5 µg/L, respectively. Separation of caffeine and paracetamol were performed on the TLC plate, and the best results were obtained using the optimized mobile phase n-hexane–ethyl acetate–ethanol (2.5 + 1.5 + 0.4, v/v). Some important parameters, such as solvent type and ratio of the mobile phase, the presence of other components, and instrumental parameters, were studied. Caffeine and paracetamol detection limits were 0.025 and 0.032 µg/L, and RSD values for 0.6 µg/L caffeine and 0.06 µg/L paracetamol (n = 5) were 1.93 and 2.06%, respectively. Using this technique, some pharmaceuticals containing caffeine and paracetamol were analyzed with satisfactory results.