bluuebunny/crossref_metadata_2025_split huggingface dataset:
m6A Methylation Regulators Are Predictive Biomarkers for Tumour Metastasis in Prostate Cancer
Authors: Wu Xiaobo , Ge Qianwen , Yang Chen , Wu Yishuo , Hu Mengbo , Ma Chenkai , Jiang Haowen , Hu Jimeng 
Published: October 2020

Abstract
BackgroundProstate cancer is one of the most common cancers in men. Usually, most prostate cancers are localized in initial diagnoses and grow slowly. Patients with localized prostate cancers have a nearly 100% 5-year survival rate; however, the 5-year survival rate of metastatic or progressive prostate cancer is still dismal. N6-methyladenosine (m6A) is the most common post-transcriptional mRNA modification and is dynamically regulated by m6A regulators. A few studies have shown that the abnormal expression of m6A regulators is significantly associated with cancer progression and immune cell infiltration, but the roles of these regulators in prostate cancer remain unclear. MethodsHere, we comprehensively examined the patterns of 21 m6A regulators across 494 prostate cancers and systematically correlated m6A regulators with prostate cancer progression and immune cell infiltration. Consensus clustering was utilized for the subtype identification of m6A regulators for prostate cancers. Each subtype signature genes were obtained by the pairwise differentially expressed genes. Featured pathways of m6A subtypes were predicted consequently. The m6A score was constructed to predict the m6A activation. The association of m6A score with patients’ survival, metastasis and immune cell infiltration were also investigated. ResultsWe identified three distinct clusters in prostate cancer based on the expression profiles of 21 m6A regulators by consensus clustering. The differential expression and pathway analyses on the three clusters uncovered the m6A regulators involved in metabolic processes and immune responses in prostate cancer. Moreover, we established an m6A score to perceive the m6A regulator activation for prostate cancer. The m6A score is significantly associated with Gleason scores and metastasis in prostate cancer. The predictive capacity of m6A score on prostate cancer metastasis was also validated in another independent cohort. ConclusionOur study revealed the critical role of m6A regulators in prostate cancer progression and m6A score is promising predictive biomarker for prostate cancer metastasis.
The circRNA DENND4C/miR-145-5p/HOXA9 Pathway Regulates Tumor Growth and Bone Metastasis in Breast Cancer
Authors: Zhu Menghai , Lian Chong , Chen Gang , Zou Peng 
Published: October 2020

Abstract
BackgroundCircular RNAs (circRNAs) are involved in the occurrence and development of breast cancer bone metastasis. This study aims to identify whether the circRNA DENND4C/ miR145-5p/HOXA9 axis is involved in the regulation of cell invasion and migration and breast cancer progression.MethodscircRNA DENND4C, miR-145-5p and HOXA9 expression was measured in serum samples from healthy volunteers, breast cancer patients without bone metastasis and breast cancer patients with bone metastasis. Moreover, we analyzed the levels of circRNA DENND4C, miR‑145-5p and HOXA9 according to different conditions of differentiation, tumor volume and lymph node metastasis. The online software starBases and the dual-luciferase reporter gene assay were used to predict the relationship miR-145-5p, circRNA DENND4C and HOXA9 mRNA. MTT assays were performed to assess the effect of circRNA DENND4C on proliferation. To assess the proliferation of breast cancer cells among different groups. Statistical significance was determined byStudent's t‑test which was used for comparisons between two groups and one‑way analysis of variance followed by Tukey's post hoc test for comparisons between more than two groups.ResultsThe expression patterns of circRNA DENND4C, miR145-5p and HOXA9 were altered in patients with breast cancer bone metastasis. Notably, the stimulatory effects of circRNA DENND4C overexpression on HOXA9 were eliminated by miR-145-5p upregulation. circRNA DENND4C overexpression promotes proliferation, migration and invasiveness by regulating the miR-145-5p/HOXA9 axis. circRNA DENND4C downregulation suppresses proliferation, cell viability and invasiveness by regulating the miR-145-5p/HOXA9 axis. ConclusionOur study suggest that circRNA DENND4C/miR-145-5p/HOXA9 pathway was involved in tumor growth and bone metastasis in breast cancer. This findings may facilitate the development of potential therapeutic agents to improve the prognosis of patients with breast cancer bone metastasis.
Actor-Critic Learning Based Energy Optimization for UAV Access-and-Backhaul Networks
Authors: Yuan Yaxiong , Lei Lei , Vu Thang X. , Chatzinotas Symeon , Sun Sumei , Ottersten Björn 
Published: October 2020

Abstract
In unmanned aerial vehicle ( UAV )-assisted networks, UAV acts as an aerial base station to serve ground users ( GUs ) through an access network, and meanwhile, the requested data traffic is acquired through the backhaul link. In this paper, we investigate an energy minimization problem with limited power supply for both backhaul and access links. The difficulties for solving such a non-convex and combinatorial problem lie at the high computational complexity/time. In solution development, we consider the approaches from both actor-critic deep reinforcement learning (AC- DRL ) and optimization perspectives. Firstly, two offline non-learning algorithms, i.e., an optimal and a heuristic algorithms, based on piecewise linear approximation and relaxation are developed as benchmarks. Secondly, towards real-time decision making, we improve the conventional AC- DRL and propose two learning schemes: AC-based user group scheduling and backhaul power allocation ( ACGP ), and joint AC-based user group scheduling and optimization-based backhaul power allocation ( ACGOP ). Numerical results show that the computation time of both ACGP and ACGOP are reduced tenfold to hundredfold compared to the offline approaches, where ACGOP is better than ACGP in energy savings. The results also verify the superiority of proposed learning solutions in terms of guaranteeing the feasibility and minimizing the system energy compared to the conventional AC- DRL .
Impact of Exports and Imports on Economic Growth in Rwanda: 1961-2018
Authors: Ruranga Charles , Ruturwa Daniel S. , Rwema Valens 
Published: October 2020

Abstract
The aim of this paper is to investigate the impact of trade on economic growth in Rwanda. This paper uses exports and imports for trade and gross domestic product for economic growth. Research questions were formulated as (1) Are exports, imports and economic growth cointegrated? (2) Is there a long or short run relationship between those Variables? (3) Are there any causal relationships between factors (4) what the direction of the causality is it? Annual time series data from World Development Indicators for the period from 1961 to 2018 have been used. The methods of linear regression for estimation of Vector Auto regressions models have been used. Our findings established that VAR was appropriate model, and GDP, Exports were stationary at first differences while Imports was stationary at second difference but not at levels. Hence the two series were integrated of order one and the third one was integrated of order two. Tests of cointegration indicates that the three variables were not cointegrated, implying there was no long run equilibrium relationship between the three series. The causality test indicated that exports and imports influenced GDP. On the other hand, we found that there was a strong evidence of unidirectional causality from exports to economic growth. However, there was bidirectional causality between GDP and imports. These results provide evidence that exports and imports, thus, were seen as the source of economic growth in Rwanda.
The Impact of Tilt Angle and Flowrate on Performance of Nanofluid Based Photovoltaic/ Thermal (PV/T) Solar Collectors
Authors: Naderi Aliasghar , Gazori Heshmatollah , Bozegi Mahan 
Published: October 2020

Abstract
Background Today, by the arrival of new sustainable energy technology, the provision of energy for the global population has turned into a significant issue for societies. Meanwhile, photovoltaic/thermal (PV/T) solar collectors, as one of the most advanced types to produce electricity and heat simultaneously, can be applied with nanofluid as the working fluid. Methods In this research, PVP coated silver nanofluid was prepared in three volume concentrations being 250, 500 and 1000 ppm by two-step method to determine the stability and thermal conductivity, experimentally. Then, the performance of PV/T solar collector is analyzed by TRNSYS software to study electrical and thermal efficiency and also output electrical and thermal energy in different months, flowrates (25, 50, 75, 100, 150 and 200 l/h) and nanofluid’s concentration. Results Based on the results, the optimum flowrate and nanofluid’s concentration are obtained 50 l/h and 1000 ppm PVP coated silver nanofluid. At last, the effect of tilt angle on the output thermal and electrical energy is determined. According to the results, by changing tilt angle in different months, the performance of PV/T solar collector can be ameliorated. Conclusion This paper can be heeded as a novel approach to overcome the lack of solar radiation in winters by improving the performance of PV/T solar collectors.
Comparative Transcriptome and WGCNA&nbsp;Reveal Candidate Genes Involved in Petaloid Stamens in Paeonia Lactiflora
Authors: Fan Yongming , Zheng Yanyi , Silva Jaime A. Teixeira da , Yu Xiaonan 
Published: October 2020

Abstract
Background: The phenomenon of petaloid stamens in Paeonia lactiflora is an important cause of double flower formation. Although research on stamen development in model plants has progressed, the molecular mechanism of P. lactiflora petaloid stamens is still unclear. Results: In this study, a comparative transcriptomic analysis was performed on two cultivars of P. lactiflora (‘Fen Yu Nu’ and ‘Lian Tai’) with different stamen developmental patterns. Using transcriptome sequencing, 89,393 unigenes were identified in P. lactiflora. Trend analysis and weighted gene co-expression network analysis (WGCNA) indicated that 18 candidate genes were likely involved in petaloid stamens, including seven MADS-box genes PlAP3, PlDEFA, PlPI2, PlAG-1, PlSEP3, PlSEP1-1, and PlSEP1-2, and 11 transcription factors (TFs) PlTCP2, PlTCP4, PlTCP9, PlbHLH36, PlICE1, PlLBD38, PlNAC083, PlBLH11, PlPDF2, PlGBF1, and PlIIIA. Based on the selected candidate genes, a hypothetical model of gene expression network regulating petaloid stamens is proposed. Conclusions: Our results provide a collection of candidate genes for the analysis of P. lactiflora petaloid stamens, allowing for in-depth studies of the development pattern of P. lactiflora flower organs, and providing a theoretical basis for related research on petaloid stamens of other herbaceous flowers.
Estimation of Relative Source Locations From Seismic Amplitude: Application to Earthquakes and Tremors at Meakandake Volcano, Eastern Hokkaido, Japan
Authors: Ogiso Masashi , Yomogida Kiyoshi 
Published: October 2020

Abstract
Although seismic amplitudes can be used to estimate event locations for volcanic tremors and other seismic events with unclear phase arrival times, the precision of such estimates is strongly affected by site amplification factors. Therefore, reduction of the influence of site amplification will allow more precise estimation of event locations by this method. Here, we propose a new method to estimate relative event locations using seismic amplitudes. We use the amplitude ratio between two seismic events at a given station to cancel out the effect of the site amplification factor at that station. By assuming that the difference between the hypocentral distances of these events is much smaller than their hypocentral distances themselves, we derive a system of linear equations for the differences in relative event locations. This formulation is similar to that of a master event location method that uses differences in phase arrival times. We applied our new method to earthquakes and tremors at Meakandake volcano, eastern Hokkaido, Japan. Comparison of the hypocentral distributions of volcano-tectonic earthquakes obtained thereby with those obtained from phase arrival times confirmed the validity of our new method. Moreover, our method clearly identified source migration among three source regions in the tremor on 16 November 2008, consistent with previous interpretations of other geophysical observations in our study area. Our method will thus be useful for detailed analyses of seismic events whose onset times are ambiguous.
SnpRecode: A Versatile and Fast Genotype Recoding and Correlation Function
Authors: Marete Andrew , Bissonnette Nathalie 
Published: October 2020

Abstract
SnpRecode is a tool meant to be implemented with the Fimpute imputation software. SnpRecode allows for fast and seamless conversion of genotypes to and from a format acceptable by Fimpute software. It also implements a fast genotype correlation function to estimate and plot the imputation accuracy. SnpRecode is implemented in Python and its source code and Linux executable is freely available for download at https://github.com/amarete/fimpute-utils
Dietary Moutan Cortex Radicis Alters Serum Antioxidant Capacity, Intestinal Immunity and Colonic Microbiota in Weaned Piglets
Authors: Bai Miaomiao , Liu Hongnan , Wang Shanshan , Shu Qingyan , Xu Kang , Zhou Jian , Xiong Xia , Huang Ruilin , Deng Jinping , Yin Yulong , Liu zheng'an 
Published: October 2020

Abstract
Background: Moutan cortex radicis (MCR), as a common traditional Chinese medicine, has been widely used as antipyretic, antiseptic and anti-inflammatory agent in China. However, few studies have evaluated the positive effects of MCR, as a new feed additives, on alleviating weaning stress and improving intestinal health and microbiom in pigs. This study aimed to investigate the effect of dietary MCR supplementation on serum antioxidant capacity, intestinal morphology, anti-inflammatory mechanism, and microbiota in weaned piglets.Results: Supplemental 2000 mg/kg and 4000 mg/kg MCR increased (P < 0.05) the final body weight, ADG and ADFI of weaned piglets, and 2000 mg/kg MCR diet significantly decreased (P < 0.05) the F/G ratio and increased (P < 0.05) serum catalase activity compared with CON group. Also, the villus height and crypt depth in the ileum and the concentrations of total SCFA, acetic acid, butyric acid and valeric acid in the colonic contents were higher (P < 0.05) in the 2000 mg/kg and 4000 mg/kg MCR diets than CON group. Dietary MCR supplementation at 4000 mg/kg MCR significantly increased (P < 0.05) total antioxidative capability and the crypt depth in the jejunum but decreased (P < 0.05) the mRNA expression levels of Interferon γ, tumor necrosis factor-α, interleukin-1β, inhibiting kappa B kinase β (IKKβ), inhibiting nuclear factor kappa-B (IκBα) and nuclear factor kappa-B (NF-κB) in the jejunum and ileum. Supplemental 8000 mg/kg MCR had the higher total antioxidative capability and catalase activity in the serum but decreased (P < 0.05) the villus height and crypt depth in the jejunum compared with the CON group. MCR addition reduced (P < 0.05) serum malondialdehyde content, and tended to increase the mRNA expression of zonula occludens-1 in the ileum (P = 0.066) compared to the CON group. Microbiota sequencing identified the microbial richness indices (Chao1, ACE, and observed species), the relative abundances of Firmicutes and Lactobacillus were increased (P < 0.05), and the relative abundances of Bacteroides, Parabacteroides, unidentified_Lachnospiraceae and Enterococcus were reduced (P < 0.05) by MCR supplemented. Microbial metabolic phenotypes analysis also showed that the richness of aerobic bacteria and facultative anaerobic bacteria, oxidative stress tolerance, and biofilm forming were significantly increased (P < 0.05), and the richness of anaerobic bacteria and pathogenic potential of gut microbiota were reduced (P < 0.05) by MCR treatment. Conclusions: In antibiotic-free diets, MCR supplementation improved growth performance and serum antioxidant capacity, alleviated intestinal inflammatory by inhibiting IKKβ/IκBα/NF-κB signaling pathway and affecting intestinal microbiota in weaned piglets.
The Landscape of Chromatin Accessibility in Skeletal Muscle During Embryonic Development in Pigs
Authors: Yue Jingwei , Hou Xinhua , Liu Xin , Wang Ligang , Gao Hongmei , Zhao Fuping , Shi Lijun , Shi Liangyu , Yan Hua , Deng Tianyu , Gong Jianfei , Wang Lixian , ZHANG Longchao 
Published: October 2020

Abstract
Background: The development of skeletal muscle during the embryonic stage in pigs is precisely regulated by transcriptional regulation, which depends on chromatin accessibility. However, how chromatin accessibility plays a regulatory role during embryonic skeletal muscle development in pigs has not been reported. To gain insight into the landscape of chromatin accessibility and the associated genome-wide transcriptome during embryonic muscle development, we performed ATAC-seq and RNA-seq on skeletal muscle of pig embryos at 45, 70 and 100 days post coitus (dpc). Results: In total, 21638, 35447 and 60181 unique regions (or peaks) were found across 45 dpc (LW45), 70 dpc (LW70) and 100 dpc (LW100) embryos, respectively. More than 91% of peaks were annotated within -1 kb to 100 bp of transcription start sites (TSSs). First, widespread increases in specific accessible chromatin regions (ACRs) from 45 to 100 dpc embryos suggested that the regulatory mechanisms became increasingly complicated during embryonic development. Second, the findings of integrated ATAC-seq and RNA-seq analyses showed that not only the numbers but also the peak intensities of ACRs could control the expression of associated genes. Finally, motif screening of stage-specific ACRs revealed some transcription factors that regulated muscle development-related genes, such as MyoD, Mef2c, Mef2d and Pax7. Several potential transcriptional repressors, including E2F6, GRHL2, OTX2 and CTCF, were identified among those genes that exhibited different change trends between the ATAC-seq and RNA-seq data. Conclusions: This work indicates that chromatin accessibility plays an important regulatory role in the embryonic muscle development of pigs and regulates the temporal and spatial expression patterns of key genes in muscle development by influencing the binding of transcription factors. Our results contribute to a better understanding of the regulatory dynamics of genes involved in pig embryonic skeletal muscle development.
Investigation on the Tribological Behaviors of as-sprayed Al2O3&nbsp;Coatings With the Effects of MoS2&nbsp;Lubricant and External Loads
Authors: Deng Wen , TANG Lin , QI Hui , ZHANG Changfu 
Published: October 2020

Abstract
The Al2O3-MoS2 ceramic-based lubricating coating was prepared via introducing MoS2 dry film lubricant into the pores and cracks of thermal-sprayed Al2O3 coating by facial paint spraying method. The microstructure, mechanical properties and tribological behaviors of the as-received Al2O3-MoS2 coating were thoroughly evaluated. The results illustrated that MoS2 was mainly concentrated on the shallow surface of the Al2O3 coating, and thus more uniform, more compact and smoother Al2O3-MoS2 coating was obtained. Meanwhile, the mechanical properties of the Al2O3 coating did not change significantly after the introduction of MoS2. The tribological experiments illustrated that compared with the Al2O3 coating, the friction coefficient and specific wear rate of the Al2O3-MoS2 coating under different loads were greatly reduced due to the generation of the lubricating layer. Especially under the load of 5 N, the friction coefficient was as low as 0.36, and the wear rate (1.49×10-5 mm3×(N×m)-1) was almost 17 times lower than that of Al2O3 coating (2.53×10-4 mm3×(N×m)-1). This research established a new and simple way to prepare ceramic-based self-lubricating coatings by using temperature-sensitive solid lubricants.
Bioprospecting of wild type ethanologenic yeast for ethanol fuel production from wastewater-grown microalgae.
Authors: Romero-Frasca Enrique , Velasquez-Orta Sharon , Escobar-Sánchez Viviana , Tinoco-Valencia Raunel , Ledesma María Teresa Orta 
Published: October 2020

Abstract
Background: Wild type yeasts have been successfully used to obtain food products, yet their full potential as fermenting microorganisms for large-scale ethanol fuel production has to be determined. In this study, wild type ethanologenic yeasts isolated from a secondary effluent were assessed for their capability to ferment saccharified microalgae sugars. Results: Yeast species in wastewater were identified sequencing the Internal Transcribed Spacers 1 and 2 regions of the ribosomal cluster. Concurrently, microalgae biomass sugars were saccharified via acid hydrolysis, producing 5.0 ± 0.3 g L-1 of fermentable sugars. Glucose consumption and ethanol production of yeasts in hydrolyzed-microalgae liquor were tested at different initial sugar concentrations and fermentation time. The predominant ethanologenic yeast species was identified as Candida sp., and glucose consumption for this strain and S. cerevisiae achieved 75% and 87% of the initial concentration at optimal conditions, respectively. Relatively similar ethanol yields were determined for both species, achieving 0.45 ± 0.05 (S. cerevisiae) and 0.46 ± 0.05 g ethanol per g glucose (Candida sp.). Conclusion: Overall, the results provide a first insight of the fermentation capacities of specific wild type Candida species, and their potential role in ethanol industries seeking to improve their cost-efficiency.