bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bioinformatics Analysis Reveals Novel Hub Gene Pathways Associated With IgA Nephropathy
Authors: Jiang xue , Xu Zhijie , Du Yuanyuan , Chen Hongyu 
Published: June 2020

Abstract
Background: Immunoglobulin A nephropathy (IgAN) is the most common primary glomerulopathy worldwide. However, the molecular events underlying IgAN remain to be fully elucidated. This study aimed to identify novel biomarkers of IgAN through bioinformatics analysis and elucidate the possible molecular mechanism. Methods: Based on the microarray datasets GSE93798 and GSE37460 downloaded from the Gene Expression Omnibus database, the diﬀerentially expressed genes (DEGs) between IgAN samples and normal controls were identified. Using the DEGs, we further performed a series of functional enrichment analyses. Protein-protein interaction (PPI) networks of the DEGs were constructed using the STRING online search tool and were visualized using Cytoscape. Next, hub genes were identified and the most important module among the DEGs, Biological Networks Gene Oncology tool (BiNGO), was used to elucidate the molecular mechanism of IgAN.Results: In total, 148 DEGs were identified, comprising 53 upregulated genes and 95 downregulated genes. Gene Oncology (GO) analysis indicated that the DEGs for IgAN were mainly enriched in extracellular exosome, region and space, fibroblast growth factor stimulus, inflammatory response, and innate immunity. Module analysis showed that genes in the top 1 significant module of the PPI network were mainly associated with innate immune response, integrin-mediated signaling pathway and inflammatory response. The top 10 hub genes were constructed in the PPI network, which could well distinguish the IgAN and control group in monocyte and tissue samples. We finally identified the integrin subunit beta 2 (ITGB2) and Fc fragment of IgE receptor Ig (FCER1G) genes that may play important roles in the development of IgAN.Conclusions: We identified key genes along with the pathways that were most closely related to IgAN initiation and progression. Our results provide a more detailed molecular mechanism for the development of IgAN and novel candidate gene targets of IgAN.
L1 Gene Molecular Variation of Human Papilloma Virus Type 16 from Cervical Cancer Patient
Authors: Savira Maya , Putra Andani Eka , S Donel , Mutia Levina , Ovinita Sarah , Yusrawati  , Lipoeto Nur Indrawaty 
Published: June 2020

Abstract
Background: Cervical cancer is the second most cancer in the world after breast cancer, this cancer is caused by infection of high risk Human Papillomavirus (HPV) type 16. It is often found in cervical cancer whose genome structure is composed of L1 proteins. L1 protein makes up the viral capsid that has an important role to infect the cervical epithelium. Several studies have found the differences in HPV nucleotides variants that lead to changes in amino acids that can disrupt the structure, the nature function of the virus itself, and ultimately lead to changes in biological functions including host immunological recognition. Variation of the L1 gene will also affect the effectiveness of existing vaccines. Methods: This research is a descriptive study conducted at the laboratory of microbiology at the Faculty of Medicine at Andalas Padang University from February to August 2018 which aims to look at the molecular variations of the L1 HPV type 16 gene and see phylogenic kinship. Results: This study obtained SNPs (Single Nucleotide Polymorphism) on all HPV 16 samples in the form of C / G (6240), A / G (6432), T / G (6686), C / T (6823) and insertion of nucleotide bases ACT (6901) ) and followed by GAT base deletions (6953) variations occurring along the observed sample isolate sequences.Conclusion: There are molecular variations of the L1 HPV type 16 gene which can cause different host immune responses. Phylogenic kinship of HPV type 16 isolate in Riau is close to Asian-American isolate.
Use of systematic reviews to justify the conduct of urology clinical trials
Authors: Shepard Samuel , Wise Audrey , Johnson Bradley S. , Vassar Matt 
Published: June 2020

Abstract
Background Given the increased amount of research being funded in the field of urology, reducing the amount of research waste is vital. Systematic reviews are an essential tool in aiding in reducing waste in research; they are a comprehensive summary of the current data on a clinical question. The aim of this study is to evaluate the use of systematic reviews as justification in conducting randomized clinical trials (RCT) in high impact urology journals. Methods On December 13, 2019, one of us (BJ) conducted a PubMed search for randomized controlled trials published in the top four urology journals according to their Google Scholar h5-index. Using a masked data extraction process each RCT was searched for systematic reviews. Then each review was evaluated for if it was justification for conducting the trial based on the context the systematic review was used.Results Of the 566 articles retrieved 281 were included. Overall 60.5% (170/281) trials cited a systematic review. We found only 47.6% (134/281) studies cited a systematic review as “verbatim” justification for conducting the trial. Regression analysis yielded a finding of statistical significance in showing a correlation of studies over medical devices were more likely to cite a systematic review than other study topics ( adjusted odds ratio 2.01, 95% CI, 1.08 - 3.73) A total of 409 different systematic review citations were recorded in the 281 trials.Conclusion Less than half of clinical trials cited a systematic review as justification for conducting the trial. If clinical trials were required to support their studies with systematic reviews we believe this would greatly reduce the amount of research waste within clinical research.
LPWAN-Based Hybrid Backhaul Communication for Intelligent Transportation Systems: Architecture and Performance Evaluation
Authors: Shahgholi Taghi , Sheikhahmadi Amir , Khamforoosh Keyhan , Azizi Sadoon 
Published: June 2020

Abstract
There are more than 1.3 billion vehicles around the world and rapidly growing which causing worldwide challenges such as congestion, huge fuel consumption, and emissions. The solution to these issues could be expansion of infrastructure or making efficient use of the current infrastructure using current technological advances by implementing Intelligent Transportation Systems (ITSs). In this paper, we proposed and explored the possibility of using cellular-based Low-Power Wide-Area Network (LPWAN) communications, LTE-M and Narrowband

Internet of Things (NB-IoT), for ITS applications. LTE-M and NB-IoT are designed to provide long-range, low power, and lowcost communication infrastructure and can be a viable promising option for immediate implementation in the real world. In order to understand the feasibility of using LPWAN for ITS, we investigated two applications with low and high delay requirements:

road traffic monitoring and emergency vehicle management and preemption. Then, the performance of using LTE-M and NB-IoT for providing backhaul communication infrastructure has been evaluated in a realistic simulation environment and compared for these two scenarios in terms of end to end latency per user. SUMO traffic simulator has been used for realistic traffic generation and a Python-based program with the ability to live data exchange with SUMO has been developed for communication performance evaluations. The simulation results demonstrate the feasibility of using LPWAN for ITS backhaul infrastructure where it was in favor of the LTE-M over NB-IoT.
Repellent effect of the caraway Carum carvi L. on the rice weevil Sitophilus oryzae L. (Coleoptera, Curculionidae)
Authors: Kłyś Małgorzata , Izdebska Aleksandra , Malejky-Kłusek Natalia 
Published: June 2020

Abstract
ObjectiveThe aim of the study was to check whether Carum carvi L. essential oil and L-carvone act on Sitophilus oryzae L. as repellents and/or insecticides, in what concentrations and after what time. ResultsCaraway essential oil and L-carvone the highest repellency showed not in the highest concentrations used in the tests (1%), but in lower concentrations, respectively 0.5% and 0.1%. Caraway essential oil in all used concentrations showed repellent effects on S. oryzae. The highest repellency (60-98%) caused caraway essential oil in concentration 0.5% after 1, 2, 3, 4 and 5 h of the research. The highest repellence of L-carvone (16-100%) resulted in concentration 0.1%. The highest mortality of S. oryzae caused 0.5% caraway essential oil.
Protease Inhibitors: Strategy to Disrupt COVID 19 Contagion
Authors: Paul Dhiman C , bhattacharjee minakshi , Sarma Manash P 
Published: June 2020

Abstract
Background:

The COVID 2019 (Corona Virus Disease) has been currently going pandemic as per WHO (World Health Organisation) situation reports. The major differentiating point in this virus is the presence of a unique furin cleavage site. Our insilico study points out to the effectiveness of a potent plant origin furin inhibitor. We exploited the aspect of the cleavage machinery of furin which is critical and indispensible for the entry of SARS-CoV-2 (Severe Acute Respiratory Syndrome –Corona Virus 2) in human cells and subsequent massive contagion. Most of the drugs are presently targeting ACEII (Angiotensin Converting Enzyme 2) receptor. But these options have injurious effects in diabetics and cardiac patients. Repurposing of old drugs are the current undergoing therapeutic measures as per the EUA (Emergency Usage Administration) of Food and Drug Administration. These aspects could be avoided if we develop a better and safer strategized drug .
Methods

In silico analysis of plant based protein analogs to inhibit furin protein.
Results

Significant interactions of proposed analogs of protease inhibitor of plant origin against furin protein. Furin inhibitors would be incredible drug targets against cancers, MERS CoV (Middle East Respiratory Syndrome) and most urgently SARS- CoV-2.
Conclusion

We report the first of its kind of furin inhibitor and believe that this major finding would fast track arenas for drug trials.
Soluble P-Selectin, Von Willebrand Factor, And Adamts13 Levels As Risk Factors Of Deep Vein Thrombosis In Cancer Patients Undergoing Chemotherapy
Authors: Setiawan Budi , Permatadewi Cecilia Oktaria , de Samakto Baringin , Bugis Ashar , Pangarsa Eko Adhi , Santosa Damai , Suharti Catharina 
Published: June 2020

Abstract
Background
There is a high number of deep vein thrombosis (DVT) incidence among cancer patients undergoing chemotherapy. Chemotherapy-induced vascular endothelial cell activation (VECA) is marked with increasing plasma levels of von Willebrand Factor (VWF) and soluble P-selectin (sP-selectin) leading to activation of endothelial cells and coagulation cascade. The biological role of a disintegrin-like and metalloproteinase with thrombospondin type 1, motif 13 (ADAMTS13) is to control the activity of VWF. The objectives of this study is to investigate the role of sP-selectin, VWF, and ADAMTS13 as risk factors for the incidence of DVT in cancer patients undergoing chemotherapy.
Methods
This prospective cohort study was conducted in Dr. Kariadi hospital, Semarang Indonesia, on 40 cancer patients. Soluble P-selectin, VWF, and ADAMS13 plasma levels were determined with enzyme-linked immunosorbent assay (ELISA) method, examined before and after chemotherapy. These patients were observed for the possibility of developing DVT during three months.
Results
Deep vein thrombosis was confirmed in 5 patients (12.5%) after a median period of 8 weeks. In patients with DVT, sP-selectin and VWF were significantly higher, while ADAMTS13 were significantly lower compared in cancer patients without DVT. Pre- and post-chemotherapy concentration of sP selectin, VWF, and ADAMTS13 could effectively predict the incidence of DVT in cancer patients undergoing chemotherapy. The levels of sP-selectin, VWF and ADAMTS13 pre-chemotherapy with cut-off point >106.7 ng/mL, >2.99 U/mL and <0,80 U/mL, respectively, had relative risk (RR) for DVT incidence being 16 (95% CI 2,06-124,25, p=0,001); 36 (95% CI 5,21-248,65, p=0,000) and 10,5 (95% CI 1,31-84,28, p=0,015), respectively, whereas the levels of sP-selectin, VWF and ADAMTS13 post-chemotherapy with cut-off point >111.7 ng/mL, >3,06 U/mL and <0,49 U/mL, respectively, had RR for DVT incidence being 8.7 (95% CI 1,01-74,39, p=0,045); 20,4 (95% CI 2,60-159,94, p=0,004) and 26,25 (95% CI 3,50-196,48, p=0,002), respectively. Pre-chemotherapy vWF levels (cut-off value >2.99 U/mL) was found to be independently predict DVT incidence with RR 11.1 (95% CI, 1.95-62.74, p=0.007).
Conclusions
Plasma levels of VWF more than 2.99 U/mL pre-chemotherapy was an independent risk factor for DVT incidence, which could be performed early and helpful for thromboprophylaxis therapy.
A case-control association study of COMT rs4680 Polymorphism with Obsessive-Compulsive Disorder and Cognitive Functions: The role of sex
Authors: hamidian sajedeh , Pourshahbaz Abbas , moradkhani atefeh , Dolatshahi Behrooz , Ananloo Esmaeil Shahsavand , Ohadi Mina 
Published: June 2020

Abstract
Background

Previous studies have emphasized the role of genetic components in obsessive-compulsive disorder (OCD) and cognitive functions. One of the most controversial markers in this area is the COMT rs4680 polymorphism. In the present study, we aimed to investigate the effect of rs4680 on susceptibility to OCD and cognitive functions with respect to the moderating role of sex.
Methods

The subjects included 127 patients with OCD and 145 healthy controls. Genotyping was carried out by ARMS-PCR. The subjects underwent cognitive evaluations using the Wechsler Memory Scale-III. Data were analyzed using SPSS 22 and R package software.
Results

The results showed a significantly higher frequency of the AA genotype in the OCD group than in the healthy control group. Cognitive assessments showed weaker Immediate Memory and General Memory performance in the patients with OCD than in the control group. No significant association was found between rs4680 and memory dimensions in the total sample, whereas analysis by sex revealed a significant association of rs4680 with Working Memory only in females.
Conclusions

The results of this study confirm previous findings about the association of the AA genotype with OCD. These findings also corroborate previous assumptions about impaired episodic memory in patients with OCD. Moreover, the association of rs4680 with Working Memory only in females endorses the hypothesis of the sexual dimorphism of the effects of this COMT gene polymorphism.
Prioritization of Human physiology Topics for medical school, what to teach? Expert Teachers' views
Authors: Awooda Hiba A , El-Mardi Abdelmoniem S. 
Published: June 2020

Abstract
Background: There has been a sustained increase in physiology knowledge in the last century that created a growing problem for educationalists and we lack general agreement on the educational content of the physiology course for medical students. Methods: This was a descriptive cross-sectional study that involved physiology teachers with different categories of academic ranking including associate professors, assistant professors, and lecturers who were working at physiology departments in different faculties of medicine on the date of the study. A standardized self-administered questionnaire was developed, pre-tested, and used for data collection from the study population. The collected data was analyzed by the use of computerized statistical package Statistical Package for the Social Sciences (SPSS) version 23.0.Results: The respondents were asked to determine the importance of the topics using three criteria: frequency of encounter, burden, and seriousness, then based on the total score; topics were divided into core (need to know), recommended (good to know), proposed (nice to know). All the contents of physiology subjects were listed and topics were prioritized accordingly. Finally, we established a list of 53 core (need to know) topics. There was no significant correlation found between the current academic status of the participants and Important of the topics. These findings provided evidence of the presence of general agreement among physiologists on the core physiology educational content that need to be taught to medical students.
Medical Students’ Attitudes towards Careers in Primary Care in Singapore
Authors: Zainal Humairah , Smith Helen Elizabeth 
Published: June 2020

Abstract
Background: Singapore needs more family doctors to care for its ageing population and their chronic conditions. To boost the recruitment of doctors within primary care, we need to better understand medical students’ attitudes and experience of General Practice and Family Medicine. While many studies have explored the facilitators and barriers to teaching undergraduate medical students in this field of medicine from the perspectives of GP teachers and trainers, few have examined students’ exposure to primary care in medical schools. Although there are works on factors influencing students’ attitudes towards primary care careers, the roles of medical schools, professional bodies and state institutions tend to be discussed independently of one another. This article explores medical students’ perceptions towards careers in primary care and how different stakeholders might collaborate in strengthening the medical school experience. Methods: Six focus groups involving 54 students from three medical schools in Singapore were conducted. Discussions focussed on their primary care experience, their professional and career aspirations, and perceptions towards the opportunities and challenges of primary care careers. Thematic analysis was used to interpret the qualitative data. Results: 15 key themes emerged from the discussions; 10 reflected key concerns of pursuing primary care careers whereas 5 others highlighted their positive aspects. The former include society’s perceptions of primary care professions as sub-standard, specialists’ negative attitudes towards family doctors, the emphasis on the lifestyle benefits of primary care careers rather than their professional characteristics, mundane case mix, limited professional opportunities, lack of continuity of care, limited consultation time, low remuneration, need for business acumen, and conflicts created by business in clinical care. However, the respondents also articulated positive views, including its lifestyle benefits, autonomy of private practice and better patient care, opportunities for entrepreneurialism and a portfolio career, breadth of clinical problems presented, and an improved future for General Practice and Family Medicine. Conclusion: The findings demonstrate that coordinated initiatives from multiple stakeholders would help to increase the attractiveness of primary care as a career choice among students. Improvements in the medical school experience will significantly enhance the prestige of General Practice and Family Medicine.
Impact of day care services on physical and cognitive functions in old people with dementia
Authors: Lin Cheng-Fu , Wu Jia-Jyun , Huang Yu-Hui , Ju Li-Ying , Lin Shih-Yi , Lin Chu-Sheng , Chou Ying-Chyi 
Published: June 2020

Abstract
Background

Day care service (DCS) provides various activities in a professionally environment to meet the old people with functional limitations. However, relatively little is known about the effects of DCS on physical and mental functions.
Methods

We used a comprehensive geriatric assessment to evaluate the changes before and after DCS among participants in a hospital-affiliated geriatric day care center. The burden of the participants’ families was also assessed.
Results

The 18 participants with a median age of 80.9 (interquartile range (IQR): 75.2–86.6 y/o) were enrolled and followed up for six months. Based on the clinical dementia rating (CDR), disease stage was very mild in 3 participants, mild in 10, moderate in 3, and severe in 2. The activities of daily living (ADL) scores of the participants improved significantly from 75 (IQR: 60.0–80.0) at baseline to 77.5 (IQR: 65.0–90.0) at the sixth month (p < 0.001), and mini-mental state examination (MMSE) scores from 15 (IQR: 11.5–20.0) to 18 (IQR: 15.8–24.0) (p = 0.026). There was a positive correlation of baseline mini-nutritional assessment-short form score and the 3-level version of the European Quality of Life-5 dimensions utility index with both ADL and MMSE scores at the six-month follow-up. In addition, the family burden scale was reduced from 22 to 15 (p = 0.002).
Conclusion

The physical and cognitive functions in old people with dementia who received DCS were improved, and their families’ stress burden was alleviated.
Nutritional status and birth outcomes among pregnant teenagers in Ghana
Authors: Gyimah Linda Afriyie , Annan Reginald Adjetey , Apprey Charles , Asamoah-Boakye Odeafo , Azanu Wisdom , Herman Lutterodt E. , Edusei Anthony Kwaku 
Published: June 2020

Abstract
Background: Inadequate nutritional status during pregnancy can lead to adverse birth outcomes but this is more so for pregnant adolescents as they require nutrients to meet their needs for growth and that of the foetus. The study examined the relationship between nutritional status and birth outcomes among Ghanaian teenagers in rural and urban districts of the Ashanti Region, Ghana.Methods In this prospective cohort study, 416 pregnant teenagers recruited at hospitals/health centres during antenatal care were followed until delivery. Data on weight, height (for body mass index), mid-upper arm circumference (MUAC), and nutrients intake using a repeated 24-hour dietary recall were determined. Blood samples were also taken and analysed for haemoglobin (Hb), serum levels of ferritin, prealbumin, vitamin A, total antioxidant capacity (TAC), C-reactive protein (CRP), and zinc protoporphyrin (ZPP). Birth weight, gestational age at birth, type of delivery, and outcome of delivery were collected as each teenager gave birth. Data were analysed using Chi-square (Fisher’s exact) analysis, t-test, two-way ANOVA and multiple linear regression. Results: 15.9% had low birth weight, 12.5% had preterm birth, 11.5% had a postpartum haemorrhage, and 3.1% neonatal deaths. Majority of the participants had inadequate intakes of energy (81.4%), protein (77.2%), vitamins A (97.1%) and E (97.7%), calcium (99.8%), iron (97.6%), folate (93.0%), zinc (83.7%), riboflavin (88.4%), thiamin (74.4%), and fiber (74.4%) intakes. Anaemia and wasting prevalence were 57.1% and 27.8%. Between-subject effects determined using Generalized Linear Modelling indicated MUAC (p=0.058), and gestational age at delivery (p=0.023) significantly affected birth weight. A multiple linear regression analysis indicated MUAC (β=0.283, p=0.002), BMI (β=0.221, p=0.015), gestational age (β=0.285, p<0.001), and ZPP (β=0.131, p=0.057) were significantly associated with birth weight (F(23, 223), P=0.008, R2=1.941).Conclusions: Inadequate nutrients intake, anaemia, and LBW were found in these pregnant teenagers. Maternal MUAC, gestational age, and serum ZPP were associated with birth weight. Interventions for improving the nutritional status of teenagers before and during pregnancy are urgently needed to reduce the risk of adverse birth outcomes.