bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Comprehensive Overview of the COVID-19 Literature: Machine Learning–Based Bibliometric Analysis
Authors: Abd-Alrazaq Alaa , Schneider Jens , Mifsud Borbala , Alam Tanvir , Househ Mowafa , Hamdi Mounir , Shah Zubair 
Published: November 2020

BackgroundShortly after the emergence of COVID-19, researchers rapidly mobilized to study numerous aspects of the disease such as its evolution, clinical manifestations, effects, treatments, and vaccinations. This led to a rapid increase in the number of COVID-19–related publications. Identifying trends and areas of interest using traditional review methods (eg, scoping and systematic reviews) for such a large domain area is challenging.ObjectiveWe aimed to conduct an extensive bibliometric analysis to provide a comprehensive overview of the COVID-19 literature.MethodsWe used the COVID-19 Open Research Dataset (CORD-19) that consists of a large number of research articles related to all coronaviruses. We used a machine learning–based method to analyze the most relevant COVID-19–related articles and extracted the most prominent topics. Specifically, we used a clustering algorithm to group published articles based on the similarity of their abstracts to identify research hotspots and current research directions. We have made our software accessible to the community via GitHub.ResultsOf the 196,630 publications retrieved from the database, we included 28,904 in our analysis. The mean number of weekly publications was 990 (SD 789.3). The country that published the highest number of COVID-19–related articles was China (2950/17,270, 17.08%). The highest number of articles were published in bioRxiv. Lei Liu affiliated with the Southern University of Science and Technology in China published the highest number of articles (n=46). Based on titles and abstracts alone, we were able to identify 1515 surveys, 733 systematic reviews, 512 cohort studies, 480 meta-analyses, and 362 randomized control trials. We identified 19 different topics covered among the publications reviewed. The most dominant topic was public health response, followed by clinical care practices during the COVID-19 pandemic, clinical characteristics and risk factors, and epidemic models for its spread.ConclusionsWe provide an overview of the COVID-19 literature and have identified current hotspots and research directions. Our findings can be useful for the research community to help prioritize research needs and recognize leading COVID-19 researchers, institutes, countries, and publishers. Our study shows that an AI-based bibliometric analysis has the potential to rapidly explore a large corpus of academic publications during a public health crisis. We believe that this work can be used to analyze other eHealth-related literature to help clinicians, administrators, and policy makers to obtain a holistic view of the literature and be able to categorize different topics of the existing research for further analyses. It can be further scaled (for instance, in time) to clinical summary documentation. Publishers should avoid noise in the data by developing a way to trace the evolution of individual publications and unique authors.
ANTIMICROBIAL EVALUATION OF ETHANOLIC EXTRACT OF SELECTED SEED SHELLS
Authors: BAIDYA MOUSHUMI , J. ANBU , AKHTAR SEMIMUL , SARKAR SIPRA , MANDAL SUDIP KUMAR 
Published: November 2020

Objective: The study was undertaken to evaluate the antimicrobial activity of ethanolic extract of polyherbal seed shells.
Methods: The seed of Momordica charantia, Manikara zapota, Emblica officinalis, Syzygium cumini, collected from the local market, Mathikere, Bangalore, India. Ethanolic extract was prepared from the dried seed powders using solvent 80% ethanol. Initially, antimicrobial activity of the extract was performed by agar well diffusion method against two bacterial strains (Escherichia coli, and Staphylococcus aureus) and two fungal pathogens (Aspergillus niger and Candida albicans).
Results: The antimicrobial study results revealed that the test extract was strongly inhibited the growth of bacteria, whereas it was not inhibited the growth of fungal organisms used in this study.
Conclusion: The results suggest that ethanolic extract of seeds possess antimicrobial properties which can be used for the treatment of infectious diseases.
“MYCO SYNTHESIS OF SILVER NANOPARTICLES USING GLIOCLADIUM ROSEUM (CLONOSTACHYS ROSEA (LINK) SCHROERS, SAMUELS) AND ITS ANTIMICROBIAL EFFICACY AGAINST SELECTED PATHOGENS”
Authors: NAIR BHARATH , ABRAHAM DELMY , DINESH AMRITA , SWAMY G. E. MALLIKARJUNA 
Published: November 2020

Objective: Antimicrobial efficacy of silver nanoparticles from Gliocladium roseum, culture filtrate (C. F.) and mycelial mat extract (M. E.) against selected pathogens.
Methods: Culture filtrate (C. F.) and Mycelial mat extract (M. E.) of Gliocladium roseum were subjected to 10 Mm silver nitrate solution for the synthesis of silver nanoparticles. Formed silver nanoparticles were evaluated via UV-vis spectroscopy and the structural elucidation was done by FT-IR and TEM. Antimicrobial efficacy was tested against bacterial (Salmonella typhi and Klebsiella pneumonia) and fungal (Cladosporium cladosporioides and Alternaria alternata) pathogens. Different nanoparticle concentrations-50, 100, 150 and 200 µl were checked via disc diffusion method.
Results: Gliocladium roseum (C. F. and M. E.) on interaction with silver nitrate solution effectively reduced metallic silver exhibiting a colour change from yellow to dark brown within 24 h due to the formation of silver nanoparticles. The UV-vis spectrum of C. F. and M. E. showed maximum absorption peaks at 350-400 nm and 400-450 nm respectively and FT-IR and TEM showed strong N-H bonding and spherical shaped silver nanoparticles with the size of 11-19 nm (C. F.) and 25-38 nm (M. E.). Antimicrobial analysis resulted in efficient inhibitory activity against Salmonella typhi, Klebsiella pneumonia and also showed moderate inhibitory activity against Alternaria alternata and Cladosporium cladopsorioides.
Conclusion: The synthesis of silver nanoparticles from fungus Gliocladium roseum is simple, cheap, safe and eco-friendly thus emphasising on large scale scientific application.
EFFECT OF MYOPIA ON PRIMARY OPEN ANGLE GLAUCOMA
Authors: REDDY MENAKURU SREYA , N. DIVYA , VEERAMANI PANIMALAR A. , BHASKARAN BINDU 
Published: November 2020

Objective: It is a retrospective study to evaluate the effect of myopia on primary open-angle glaucoma by classifying the eyes into NMG (non-myopic glaucoma), HMG (highly myopic glaucoma) And MMG (mild to moderate myopic glaucoma).
Methods: The study was performed on 120 patients with primary open-angle glaucoma who were medically treated. Any participant who had surgery done was excluded from the study. The relation between glaucoma and different myopia and progression were assessed on the basis of age, gender, risk factors.
Results: Out of the 120 cases assessed, 46 [38 percent] were female and 74 [62 percent] were males. On the basis of age there were 9 cases between 31-40 y, 30 cases between the ages 41-50, 40 cases between 51-60, 32 cases between 61-70 and 9 cases between 71-80, on the basis of myopia 59 [49 percent] were NMG 47 [39 percent] were MMG and 14 [12 percent] were HMG. On the basis of risk factors,12 of them had Diabetes Mellitus, 7 had Hypertension, 7 had a history of steroid use, 3 had a history of migraines and 6 of them had a family history of glaucoma. In the observed one year period 73 percent if the cases were not progressive while 27 percent were progressive. In this study, it has been observed that the males are more commonly affected and the age group with the most cases was the 51 to 60 age group. The majority of the cases showed no risk factors though Diabetes Mellitus is the most common. The progression of the disease is seen more frequently in cases associated with Diabetes Mellitus and Hypertension. In MMG 12 out of the 47 cases were progressive and in NMG 15 out of the 59 cases were progressive.
Conclusion: Though high myopia is important in the pathogenesis of glaucoma there was no evidence that high myopia increases the progression of the disease of the 14 cases, only 5 were progressive.
Научно-инновационный комплекс как один из базовых факторов трансформации каркаса расселения на примере СТП Свердловской области. Предпосылки включения научно-инновационного комплекса в базовые факторы трансформации региональной системы расселения
Authors: Kuleshova Galina 
Published: November 2020

Вызовы, вставшие перед страной в связи с переходом экономики страны на инновационный путь, выводят на новый уровень градоформирующую роль научно-инновационного комплекса страны.
Системы расселения регионов РФ вкупе с террито­риальной структурой экономики формировались пре­имущественно на индустриальном этапе развития страны в тесном взаимодействии с производством. При переходе к рыночным принципам организации хозяйства структура экономики регионов, в значительной мере разрушенная в 90-е годы прошлого века, претерпела существенные измене­ния, что обусловило высокую степень пространственной не­однородности экономического роста в регионах, изменение демографической ситуации в муниципальных образованиях, стало причиной деградации части внутрирегиональных систем расселения. Инновационный путь развития меняет роль различных типов поселений в экономике, предъяв­ляя новые требования и к качеству жизни на территории, и к качеству трудовых ресурсов на местном рынке труда. Трансформация экономики, ориентированной на модерниза­цию и инновационный путь развития, должна осуществлять­ся с учётом обеспечения условий для диффузии инноваций на пространственном уровне. Вопросы интенсификации инновационной деятельности приобретают важное значение для повышения качества устойчивого развития как в целом Свердловской области, так и её промышленно-производ­ственного каркаса. Основным драйвером преобразований является научно-инновационный комплекс региона, а местом активизации - территории его преимущественного размещения и развития[1].

[1] Настоящая статья написана на основе раздела «Научно-инновационный комплекс Свердловской области» НИР «Внесение изменений в схему террито­риального планирования Свердловской области» (ОАО «ГИПРОГОР», Москва, 2020 год. Заказчик: Министерство строительства и развития инфраструктуры Свердловской области, госконтракт № ОКЭФ-2019-07 от 28.10.2019).
Morphological evaluation of the distal medial striate artery. A study with cadaveric material.
Authors: García Natalia , Forero Porras Pedro Luis , Ballesteros Acuña Luis Ernesto 
Published: November 2020

SUMMARY:
Background: The distal medial striated artery (DMSA) is a small, constant branch that emerges from the base of the anterior cerebral artery (ACA) and is directed backwards to irrigate internal structures such as the nucleous of the base.
Objective: This study proposes to evaluate the morphology of the DMSA, useful for clinical and surgical management that compromise this vascular structure.
Methods: The DMSA of 71 unclaimed male bodies, who underwent necropsy at the Institute of Legal Medicine and Forensic Sciences of Bucaramanga-Colombia, were evaluated using the perfusion technique of vascular structures with polyester resin. Results: The DMSA was presented in four cases (2.8%) duplicated and with agenesis in 2.1% of the hemispheres. Its origin was 43.9% of the post-communicating segment of the ACA (A2), 10.8% of the pre-communicating segment of the ACA (A1) and 44.6% of the ACA junction site with the anterior communicating artery (AComA). The diameter of the DMSA was 0.5 ± 0.2 mm and its total length was 20.3 ± 4.1 mm. Sinuous trajectory was observed in 73 cases (51.4%). There was a superolateral relationship with the ACA in 48% of the samples. The DMSA was observed in 129 cases (90.9%) as a single trunk while in 6 cases (4.2%) it presented with a common trunk with the orbitofrontal artery (OF). The completion of the DMSA in the anterior perforated substance was given by a single trunk in 129 cases (90.9%) bifurcation in 9 cases (6.3%) and trifurcation in four cases (2.8%). The incidence of sinuous presentation and hypoplasia of the DMSA is considerably higher than previously reported.
Conclussions: The importance of the DMSA lies in its involvement with the surgical procedures and interventions of the anterior segment of the arterial cerebral circle, and in the complex clinical management that determine its injury in clinical practice.
Alpha-synuclein gene mRNA and protein level in cd45+ cells in patients with multiple systemic atrophy
Authors: Николаев М.А. , Усенко Т.С. , Безрукова А.И. , Богданова Д.А. , Сенкевич К.А. , Милюхина И.В. , Пчелина С.Н. 
Published: November 2020

Множественная системная атрофия (МСА) - редкое нейродегенеративное заболевание, относящееся к группе заболеваний, ассоциированных с агрегацией белка альфа-синуклеина. Самой распространенной синуклеинопатией является болезнь Паркинсона (БП). Молекулярный механизм МСА и БП остается неизвестным, в связи с чем неизвестны диагностические биомаркеры, способные дифференцировать эти заболевания на ранней стадии. В данном исследовании был оценен уровень мРНК и белка альфа-синуклеина в CD45+ клетках периферической крови у пациентов с МСА, БП и в контроле. Выявленное значительное повышение экспрессии гена альфа-синуклеина у пациентов с МСА по сравнению с пациентами с БП (p<0,0001) и контрольной группой (p<0,0001). Уровень мРНК гена альфа-синуклеина CD45+ клеток крови может рассматриваться как потенциальный биомаркер МСА.
Multiple system atrophy (MSA) is a rare neurodegenerative disorder that belongs to a group of neurodegenerative diseases called synucleinopathies. The most common synucleinopathy is Parkinson’s disease (PD). The molecular mechanism of MSA and PD remains unknown, and therefore diagnostic biomarkers that can differentiate these diseases at an early stage are unknown. The aim of this study was to estimate the level of mRNA and alpha-synuclein protein in CD45+ peripheral blood cells of patients with MSA, PD, and control. The significant increase of alpha-synuclein expression in MCA patients compared to patients with PD (p<0.0001) may be considered as a potential biomarker of this disease.
The role of presynaptic cytomatrix protein Piccolo and inflammatory genes in the development of depressive behavior
Authors: Давыдова Ю.Д. , Казанцева А.В. , Еникеева Р.Ф. , Мустафин Р.Н. , Лобаскова М.М. , Малых С.Б. , Гилязова И.Р. , Хуснутдинова Э.К. 
Published: November 2020

Целью настоящего исследования являлась оценка основных эффектов полиморфных локусов генов IL1B (rs16944), TNF (rs1041981), CRP (rs3093077) и PCLO (rs2715157), а также ген-средовых взаимодействий в фенотипических вариациях уровня депрессивности у 999 психически здоровых индивидов с учётом половой и этнической принадлежности. Статистический анализ выявил ассоциации аллеля rs1041981*A гена TNF с пониженным уровнем депрессивности в группе индивидов русской этнической принадлежности (р=0,04) и аллеля rs2715157*A гена PCLO с повышенными показателями в этнической группе татар (рFDR=0,03). В результате стратификационного анализа был выявлен значимый модулирующий эффект таких средовых факторов, как место жительства и сезон рождения, в случае ассоциации полиморфных локусов rs16944, rs1041981 и rs2715157 с уровнем депрессивности.
The present study aimed to assess the main effects of IL1B (rs16944), TNF (rs1041981), CRP (rs3093077) and PCLO (rs2715157) gene polymorphisms together with G×E effects on individual differences in depression level in 999 mentally healthy individuals with sex and ethnicity as covariates. Statistical analysis revealed an association of TNF rs1041981 A-allele with a decreased depression level in Russians (p=0.04), while PCLO rs2715157 A-allele was associated with an increased depression level in Tatars (pFDR=0.03). As a result of stratification analysis we observed that place of residence and birth season significantly affected association of rs16944, rs1041981 and rs2715157 and depression level.
Atypical manifestation of familial laminopathy with simultaneous damage to the cardiac, muscle and peripheral nervous systems
Authors: Фомичева Ю.В. , Вершинина Т.Л. , Семенов А.П. , Костарева А.А. 
Published: November 2020

Ядерные ламины являются основными белками ядерной оболочки и обеспечивают прочность ядерной мембраны и взаимодействие внеядерных структур с компонентами ядра клетки. Несмотря на большое количество исследований в данной области, нет единого мнения о механизме, лежащем в основе патогенеза ламинопатий. Мутации в гене ламина A/C являются причиной развития нескольких клинических фенотипов, как с изолированным вовлечением кардиальной, мышечной, жировой и костной тканей, так и с их сочетанием. Наиболее характерными клиническими проявлениями ламинопатий являются нарушения проводимости, желудочковые и наджелудочковые нарушения ритма, а также систолическая дисфункция миокарда. Среди пациентов, перенесших трансплантацию сердца, примерно 10% составляют пациенты с кардиомиопатиями, вызванными мутациями в гене ламина A/C. Часто доминирующая патология сердечно-сосудистой системы (ССС) может маскировать субклиническое вовлечение других тканей. Хотя сочетание кардиомиопатии с аритмическим расстройством и патологией скелетных мышц не является редкостью, о сопутствующем поражение периферической нервной системы сообщается не часто. В статье мы представляем случаи ламинопатии с различным вовлечением сердечной, мышечной и периферической нервной систем у членов одной семьи.
Nuclear laminates are the main proteins of the nuclear membrane and provide the strength of the nuclear membrane and the interaction of extranuclear structures with the components of the cell nucleus. Despite the large number of studies in this area, there is no consensus on the mechanism underlying the pathogenesis of laminopathies. Mutations in the lamin A/C gene are the cause of the development of several clinical phenotypes, both with isolated involvement of cardiac, muscle, adipose and bone tissues, and with their combination. The most characteristic clinical manifestations of laminopathies are conduction disorders, ventricular and supraventricular rhythm disturbances, as well as systolic myocardial dysfunction. Among patients who underwent heart transplantation, approximately 10% are patients with cardiomyopathies caused by mutations in the lamin A/C gene. Often, the dominant pathology of the cardiovascular system (CVS) can mask the subclinical involvement of other tissues. Although a combination of cardiomyopathy with arrhythmic disorder and skeletal muscle disease is not uncommon, concomitant damage to the peripheral nervous system is not often reported. In this article, we present family cases of laminopathy with various involvement of the cardiac, muscle and peripheral nervous systems among members of the same family.
Intermediate alleles of HTT gene in patients with Parkinson’s disease
Authors: Гомбоева Д. Е. , Брагина Е.Ю. , Никитина М.А. , Жукова Н.Г. , Алифирова В.М. , Назаренко М.С. , Пузырев В.П. 
Published: November 2020

Болезнь Гентингтона (БГ) - нейродегенеративное заболевание, причиной которого является экспансия числа CAG-повторов в первом экзоне гена HTT. Превышение порога в 36 повторов приводит к БГ. Диапазон от 27 до 35 CAG-повторов составляют так называемые промежуточные аллели, которые, согласно последним данным, модифицируют клинические проявления нейродегенеративных заболеваний. В данном исследовании выявлено два случая носительства промежуточных аллелей с 27 CAG- повторами у пациентов с болезнью Паркинсона (БП). Анализ клинической картины выявил «нетипичность» клинического проявления БП. Таким образом, промежуточные аллели гена HTT оказывают модифицирующее влияние на течение БП.
Huntington’s disease (HD) is a neurodegenerative disease, caused by a CAG-repeat expansion in exon 1 of the HTT gene. The number of repeats more than 36 leads to HD. The range of 27-35 CAG-repeats is called as intermediate alleles (IAs). There is a growing evidence of importance of IAs for patients with other neurodegenerative diseases. In this study we have detected two cases of carriage of IAs in patients with Parkinson’s disease (PD). The analysis of clinical picture has revealed atypical clinical features of PD in these individuals. Thus, IAs of HTT gene may provide a modifying effect on clinical features of PD.
Analysis of mutations spectrum in the KCNQ1, KCNH2 and SCN5A genes in patients with long QT syndrome using massively parallel sequencing
Authors: Сивцев А.А. , Свинцова Л.И. , Плотникова И.В. , Жалсанова И.Ж. , Постригань А.Е. , Минайчева Л.И. , Джаффарова О.Ю. , Скрябин Н.А. 
Published: November 2020

Проведен поиск мутаций в генах KCNQ1, KCNH2 и SCN5A методом массового параллельного секвенирования (МПС) у 10 пациентов из 8 семей с диагнозом «синдром удлиненного интервала QT» (СУИQT). Для пробоподготовки использована методика целевого обогащения участков ДНК, относящихся к исследуемым генам. В результате проведенной работы выявлено 8 мутаций: 5 из них расположены в гене KCNQ1, 2 мутации - в гене KCNH2, 1 мутация - в гене SCN5A. Во всех случаях были найдены уникальные мутации, не повторяющиеся у неродственных пациентов. Результаты проведенной работы указывают на эффективность использования таргетных панелей для поиска генетических аномалий при СУИQT.
We searched for mutations in the KCNQ1, KCNH2 and SCN5A genes using mass parallel sequencing (MPS) in 10 patients from 8 families with a diagnosis of “long QT syndrome” (LQTS). For sample preparation, we used the targeted enrichment of DNA regions method related to the studied genes. As a result of the work, 8 mutations were revealed: 5 of them are located in the KCNQ1 gene, 2 mutations in the KCNH2 gene, 1 mutation in the SCN5A gene. In all cases, we found unique mutations that did not recur in unrelated patients. The results of this work indicate the effectiveness of using targeted panels to search for genetic abnormalities in LQTS.
Personalized approach to drug therapy for multimorbid patients with left ventricular non-compaction cardiomyopathy
Authors: Блохина А.В. , Ершова А.И. , Мешков А.Н. , Киселева А.В. , Мясников Р.П. , Куликова О.В. , Зятенкова Е.В. , Скирко О.П. , Климушина М.В. , Сотникова Е.А. , Курилова О.В. , Покровская М.С. , Драпкина О.М. 
Published: November 2020

Некомпактный миокард левого желудочка (НМЛЖ) характеризуется развитием ряда осложнений, что в сочетании с сопутствующими заболеваниями обычно приводит к полипрагмазии. Фармакогенетическое тестирование (ФГТ), направленное на выявление вариантов генов, ассоциированных с метаболизмом лекарственных препаратов (ЛП), может позволить подобрать наиболее эффективную и безопасную лекарственную терапию в каждом конкретном случае. В исследование было включено 16 больных с НМЛЖ, его осложнениями и другими заболеваниями. Всем пациентам проводилась оценка 60 однонуклеотидных полиморфизмов (SNP) с помощью полимеразной цепной реакции в реальном времени в амплификаторе QuantStudio 12KFlexReal-TimePCRSystem (ThermoFisherScientific, США). По результатам ФГТ выявлено 62,5% пациентов с НМЛЖ, генотипы которых ассоциированы с изменением метаболизма ЛП. У 12,5% пациентов в анамнезе прием ЛП, требующих коррекции дозы или замены на другой ЛП с учетом результатов ФГТ.
A left ventricular non-compaction cardiomyopathy (LVNC) is characterized by the development of a number of complications, which in combination with concomitant diseases usually leads to polypharmacy. Pharmacogenetic testing (PGT), aimed at identifying variants of genes associated with the metabolism of drugs, allows to choose the most effective and safe drug therapy in each case. The study includes 16 patients with LVNC, its complications and other diseases. All patients were assessed for 60 single nucleotide polymorphisms (SNPs) using real-time polymerase chain reaction in a QuantStudio 12KFlexReal-TimePCRSystem thermocycler (ThermoFisher Scientific, USA). According to the results of PGT 62.5% of the patients with LVNC and genotypes associated with a change in the metabolism of drugs were revealed. 12.5% of the patients had a history of taking drugs that required dose adjustment or replacement with another drug, taking into account the results of PGT.