bluuebunny/crossref_metadata_2025_split huggingface dataset:
SARS-CoV-2 and the Eye: Implications for the Retina Specialist From Human Coronavirus Outbreaks and Animal Models
Authors: Kennedy Alasdair , Shantha Jessica G. , Olivia Li Ji-Peng , Faia Lisa J. , Hartley Caleb , Kuthyar Sanjana , Albini Thomas A. , Wu Henry , Chodosh James , Ting Daniel S.W. , Yeh Steven 
Published: July 2020

Purpose: The current severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has escalated rapidly since December 2019. Understanding the ophthalmic manifestations in patients and animal models of the novel coronavirus may have implications for disease surveillance. Recognition of the potential for viral transmission through tear film has ramifications for patients, physicians, and the public. Methods: Information from relevant published journal articles was surveyed using a computerized PubMed search and public health websites. We summarize knowledge of ophthalmic manifestations of SARS-CoV-2 infection in patients and animal models, risk-mitigation measures for patients and providers, and implications for retina specialists. Results: SARS-CoV-2 is efficiently transmitted among humans, and although the clinical course is mild in most infected patients, severe complications including pneumonia, acute respiratory distress syndrome, and death can ensue, most often in elderly patients and individuals with comorbidities. Conjunctivitis occurs in a minority of patients with COVID-19, and SARS-CoV-2 RNA has been identified primarily with conjunctivitis. Uveitis has been observed in animal models of coronavirus infection, and cotton-wool spots have been reported recently. Conclusions: SARS-CoV-2 and other coronaviruses have been rarely associated with conjunctivitis. Identification of SARS-CoV and SARS-CoV-2 RNA in the tear film of patients and its highly efficient transmission via respiratory aerosols supports eye protection, mask, and gloves as part of infection prevention and control recommendations for retina specialists. Disease surveillance during the COVID-19 pandemic may also include ongoing evaluation for uveitis and retinal disease given prior findings from animal models and a recent report of retinal manifestations.
Pulmonary arterial hypertension-associated changes in gut pathology and microbiota
Authors: Sharma Ravindra K. , Oliveira Aline C. , Yang Tao , Kim Seungbum , Zubcevic Jasenka , Aquino Victor , Lobaton Gilberto O. , Goel Ruby , Richards Elaine M. , Raizada Mohan K. 
Published: July 2020

Emerging evidence implicates an interplay among multiple organs such as brain, vasculature, gut and lung in the development of established pulmonary arterial hypertension (PAH). This has led us to propose that activated microglia mediated-enhanced sympathetic activation contributes to PAH pathophysiology. Since enhanced sympathetic activity is observed in human PAH and the gut is highly innervated by sympathetic nerves that regulate its physiological functions, we hypothesized that PAH would be associated with gut pathophysiology.A monocrotaline rat model of PAH was utilized to investigate the link between gut pathology and PAH. Haemodynamics, histology, immunocytochemistry and 16S RNA gene sequencing were used to assess cardiopulmonary functions, gut pathology and gut microbial communities respectively.Monocrotaline treatment caused increased right ventricular systolic pressure, haemodynamics and pathological changes associated with PAH. PAH animals also showed profound gut pathology that included increased intestinal permeability, increased muscularis layer, decreased villi length and goblet cells. These changes in gut pathology were associated with alterations in microbial communities, some unique to PAH animals. Furthermore, enhanced gut–neural communication involving the paraventricular nucleus of the hypothalamus and increased sympathetic drive were observed.In conclusion, our data show the presence of gut pathology and distinct changes in gut microbiota and increased sympathetic activity in PAH. They suggest that dysfunctional gut–brain crosstalk could be critical in PAH and considered a future therapeutic target for PAH.
Amyloidosis of the respiratory system: 16 patients with amyloidosis initially diagnosedante mortemby pulmonologists
Authors: Yamada Masami , Takayanagi Noboru , Yamakawa Hideaki , Ishiguro Takashi , Baba Tomohisa , Shimizu Yoshihiko , Okudela Koji , Takemura Tamiko , Ogura Takashi 
Published: July 2020

BackgroundAnte mortemdiagnosis of amyloidosis of the respiratory system is rare. Few data are available regarding clinical presentation, precursor proteins, diagnostic procedures, comorbidities, complications, and outcome. We assessed clinical features of a series of patients with amyloidosis of the respiratory system in two Japanese centres.MethodsMedical records of 16 patients with amyloidosis of the respiratory system were retrospectively analysed. Amyloid was diagnosed by polarisation microscopy using Congo red-stained tissue specimens and classified immunohistochemically.ResultsMedian patient age was 71 years, and median follow-up period was 5 years. Immunoglobulin light-chain (AL)-λ amyloidosis was found in eight and AL-κ in five patients. Two patients harboured wild-type transthyretin and one harboured serum amyloid A-derived amyloid. Five different forms of amyloidosis of the respiratory system were observed: nodular pulmonary amyloidosis (seven patients), diffuse alveolar-septal amyloidosis (five), mediastinal lymph node amyloidosis (three), tracheobronchial amyloidosis (one), and pleural amyloidosis (one). One patient had diffuse alveolar-septal amyloidosis and mediastinal lymph node amyloidosis. Three of five patients with diffuse alveolar-septal amyloidosis were diagnosed by transbronchial lung biopsy as having concurrent diffuse alveolar haemorrhage or pneumocystis pneumonia. Two of three patients with mediastinal lymph node amyloidosis were diagnosed by endobronchial ultrasound-guided transbronchial needle aspiration.ConclusionsNot only nodular pulmonary amyloidosis, diffuse alveolar-septal amyloidosis, and tracheobronchial amyloidosis but also mediastinal lymph node amyloidosis and pleural amyloidosis should be considered in the differential diagnosis of amyloidosis of the respiratory system. Useful diagnostic methods include transbronchial lung biopsy for diffuse alveolar-septal amyloidosis and endobronchial ultrasound-guided transbronchial needle aspiration for mediastinal lymph node amyloidosis.
Influence of age, sex and hospitalisation on the administration of tuberculosis medication: an evaluation of routine data from a German health insurer
Authors: Kersten Jan F. , Wobbe-Ribinski Stefanie , Diel Roland , Nienhaus Albert , Schablon Anja 
Published: July 2020

BackgroundSo far, there is no reliable information on the drugs actually taken by tuberculosis patients. With billing data from a large German health insurance company, valid data from practice will be used for analysis. The objective here is to use the claims data of a health insurer to gain an insight into the prescriptions issued to patients with tuberculosis in Germany.MethodsThe study design encompasses a longitudinal, analytical observational study of selected insurance holders. Descriptive analyses of the outpatient drug supply of pulmonary tuberculosis patients are determined for 6 payroll years. We have studied whether different doses of tuberculosis medication are associated with age, sex, inpatient status and comorbidity. Quantile regression is used as a method to identify subgroups or characteristic dosages.ResultsThe number of defined daily doses prescribed per patient varies among insurance holders and encompasses widely differing timeframes. Higher doses are observed with increasing age, as well as in patients with tuberculosis-related hospitalisations. The sex of the patient has no identifiable effect on the prescribed doses for any of the first-line tuberculosis drugs. Comorbidity partially has a significant impact on the duration and intensity of tuberculosis drug prescriptions.
Spontaneous hyphema associated with ocular syphilis
Authors: Deibert Brent , Wark Kellie , Diaz Rocio , Blodi Christopher 
Published: July 2020

AbstractThe purpose of this case series is to illustrate a novel presentation of ocular syphilis. Two cases of ocular syphilis presenting with spontaneous hyphema are described, demonstrating that spontaneous hyphema can be caused by ocular syphilis. This association has not previously been reported.
The antibacterial activity and toxin production control of bee venom in mouse MRSA pneumonia model
Authors: Kong Ryong , Lee Young-Seob , Kang Dam-Hee , Wang Shu , Li Qianqian , Kwon Dong-Yeul , Kang Ok-Hwa 
Published: July 2020

Abstract
Background
The current antimicrobial therapy is still important for the treatment of pneumonia due to MRSA infection, but there are some limitations, including the route of administration, side effect profile, and increased microbial resistance patterns. Therefore, we investigated whether BV, which shows a strong antimicrobial effect against MRSA, would be effective in a pneumonia model.

Methods
In vitro, we checked MIC, qRT-PCR, western blot, ELISA, LDH-assay. In vivo, we checked survival rate, gross pathological change, histopathology, lung bacterial clearance assay, and the expression of inflammatory related gene.

Results
The minimum inhibitory concentration of BV against MRSA is 15.6 μg/ml by broth dilution method. The production of toxins and related gene were reduced by BV in MRSA. The secretion of cytokines were decreased by treatment with BV in 264.7 RAW macrophages stimulated by MRSA Also, BV protected A549 from pathogenicity of MRSA. Bee venom reduced the number of bacteria in the lungs and alleviated the symptoms of MRSA-induced pneumonia in mouse.

Conclusion
BV inhibited the virulence of the bacterium and the number of bacterial cells present in lung tissue, thereby alleviating the symptoms of pneumonia in mice. This study suggested that BV may be a candidate substance for the treatment of pneumonia caused by MRSA infection.
Surgical approach strategies for open reduction internal fixation of closed complex tibial Pilon fractures based on axial CT scans
Authors: Zhao Yu , Wu Jian , Wei Shijun , Xu Feng , Kong Changwang , Zhi Xiaosong , Huang Ming , Cai Xianhua 
Published: July 2020

Abstract
Background
To explore the application and clinical efficacy of surgical approach strategies in open reduction internal fixation of closed complex tibial Pilon fractures based on axial CT scans.

Methods
This retrospective cohort study included data of 25 patients with closed complex tibial Pilon fractures treated from October 2011 to March 2014, including 19 males and 6 females aged 18–54 years (average 39.5 years). According to classification criteria of the Association for Osteosynthesis/Orthopedic Trauma Association (AO/OTA), 4 patients were type 43C1, 10 type 43C2, and 11 type 43C3. Surgical approaches were selected based on fracture line distribution and bone displacement revealed by axial CT scans, and an open reduction method was adopted for internal fixation of the bone plates. Postoperatively, Burwell-Charnley radiographic criteria were used to determine fracture reduction quality. Functional evaluation was performed using the American Orthopedic Foot and Ankle Society (AOFAS) Ankle-Hindfoot Scale. Complications, fracture union time, and the AOFAS scores at last follow-up were recorded.

Results
The 25 included patients were followed for 22–60 months postoperatively (average follow-up 33.9 months). Of these, 19 patients achieved anatomical reduction of the articular surface, 5 achieved good reduction, and one achieved fair reduction. Two patients developed superficial infection on the anteromedial incision and delayed union but recovered well after local dressing change and oral administration of antibiotics. Another patient developed deep infection on the anterolateral incision, which was controlled by debridement, catheter irrigation, and intravenous antibiotic injection. All fractures healed well and average union time was 2.8 months (range, 2–3 months). No fracture malunion or internal fixation failures were found at last follow-up. All 25 patients had AOFAS scores ranging from 80 to 100 at last follow-up (average 88.4). Overall, 15 patients were excellent, 10 good, and 0 fair or poor, with excellent and good rates of 100%.

Conclusions
Surgical approach strategies for complex tibial Pilon fractures based on axial CT scans accurately reconstruct the articular surface and achieve solid internal fixation of assembled locking plates, while early postoperative functional exercises contribute to the functional recovery of affected limbs and reduce related complications.
Intimate partner violence during pregnancy and risk of still birth in hospitals of Tigray region Ethiopia
Authors: Gebreslasie Kahsay Zenebe , Weldemariam Solomon , Gebre Gelawdiyos , Mehari Mihret-Ab 
Published: July 2020

AbstractBackgroundPregnancy may represent a time of exceptional vulnerability to intimate partner violence because of changes in women’s conditions. Despite the fact that intimate partner violence during pregnancy confers considerable risk to the health of the woman and her fetus, data regarding to association of stillbirth and intimate partner violence is lacking in Tigray region. The objective of this study is to assess intimate partner violence during pregnancy and its association with still birth among postpartum mothers in hospitals in Tigray Region of Ethiopia.MethodsCross-sectional study design was used to assess 648 women about intimate partner violence during pregnancy and its association with still birth. Simple random sampling technique was employed to select health facilities and systematic sampling was used to select the study participants. Data was entered by using Epi info version 3.5.1 and analyzed using SPSS version 20. Logistic regression analysis was done to assess the association between exposure to intimate partner violence during pregnancy and stillbirth while adjusting for possible confounders.ResultsThe prevalence of still birth was 3.6%in this study population. There was a statistically significant association between exposure to intimate partner violence during pregnancy and still birth. Pregnant women who were exposed to intimate partner violence during pregnancy were three times more likely to have still birth 3.3(95% CI: 1.1–9.7) as compared to those who were not exposed. Another important factor associated with stillbirth was low birthweight 16.7(95% CI,6–46).ConclusionsThe prevalence of still birth in this study was high. Women who subjected to intimate partner violence during pregnancy had greater risk of having stillbirth baby.
Twenty years of transposable element analysis in the Arabidopsis thaliana genome
Authors: Quesneville Hadi 
Published: July 2020

AbstractTransposable elements (TEs) are mobile repetitive DNA sequences shown to be major drivers of genome evolution. As the first plant to have its genome sequenced and analyzed at the genomic scale,Arabidopsis thalianahas largely contributed to our TE knowledge.The present report describes 20 years of accumulated TE knowledge gained through the study of theArabidopsisgenome and covers the known TE families, their relative abundance, and their genomic distribution. It presents our knowledge of the different TE family activities, mobility, population and long-term evolutionary dynamics. Finally, the role of TE as substrates for new genes and their impact on gene expression is illustrated through a few selected demonstrative cases. Promising future directions for TE studies in this species conclude the review.
Uganda’s experience with debt and economic growth: an empirical analysis of the effect of public debt on economic growth—1980–2016
Authors: Ssempala Richard , Ssebulime Kurayish , Twinoburyo Enoch 
Published: July 2020

AbstractBackgroundEmpirical evidence on the effect of public debt on the economic growth of a country remains ambiguous. No theoretical convergence on the respective nexus has been attained. For the case of Uganda in particular, the public debt question remains critical in the country’s development trajectory. Under the Highly Indebted Poor Countries (HIPCs) initiative, Uganda was the first country to receive a debt relief of worth US$650 million in the 1990s and later in 2006, under the Multilateral Debt Relief Initiative (MDRI), the country generously received 100% debt forgiveness/cancelation which consequently reduced the stock of country’s debt to $1.6 billion. However, of recent, the debt stock has kept on increasing from UGX 14.257 trillion ($5.5 billion) in 2000 to the current UGX 35.3 trillion (9.8b) in July 2017 and it is projected to continue increasing in the short to medium term given the robust NDPII core projects and priorities which are set to attract more borrowing. The study employs the Auto Regressive Distributed Lag (ARDL)-bounds testing approach which is superior and suitable for our small sample.ResultsThe results reveal that public debt has a significant negative impact on economic growth in short run whereas in long-run debt has a mixed impact on Uganda’s economy. The total debt service has a negative impact whereas Gross debt as a share of GDP has a positive impact on the economy. The findings also reveal that Public debt has a negative effect on Uganda’s economic growth in the short run. The impact is however found to be positive in the long run. This result is in line with the study expectations and some findings by earlier researchers who found a negative impact of public debt on GDP and investment. The results suggest that the current trend of Uganda’s borrowing is to continue constraining the resources in the short run.ConclusionThe conclusion of the study in view of emerging findings especially on debt, various policy implications have emerged. At the current rate of borrowing, Uganda is likely to have deteriorating economic growth partly because such public borrowing adversely affects investment. The study thus recommends for policies geared toward efficient use of borrowed funds especially for such projects that have high potential to unlock the production capabilities of the country. There is a need for the government of Uganda to institute mechanisms to ensure efficient use of borrowed funds.
Compositional variations along the route of Chang’e-3 Yutu rover revealed by the lunar penetrating radar
Authors: Ding Chunyu , Xiao Zhiyong , Su Yan , Zhao Jiannan , Cui Jun 
Published: July 2020

AbstractUsing the high-frequency lunar penetrating radar data obtained by the Chang’e-3 mission, we apply the frequency-shift method to calculate the decay rate of the electromagnetic wave in the regolith-like ejecta deposits of the Ziwei crater. The radar data are divided into segments according to the navigation points along the traverse route of the Yutu rover. For each segment, we calculate the bulk loss tangent of materials within the top ~ 50 ns of the radar data based on the frequency decreasing rate of the electromagnetic wave. The loss tangent varies from ~ 0.011–0.017 along the route of Yutu, and it is within the range of the measured loss tangent of Apollo regolith samples. Using the empirical relationship between loss tangent and TiO2 + FeO content derived from the Apollo lunar samples, we estimate the TiO2 + FeO content for the bulk regolith along the route of Yutu, which is ~ 23–30 wt.%. This value is comparable with that estimated using both orbital reflectance spectral data and in situ observation made by the Yutu rover. The loss tangent derived along the route of Yutu is larger than the average value of returned lunar samples, which is mainly caused by the larger content of TiO2 + FeO at the landing site compared to the global average. Variations of the TiO2 + FeO content along the route of Yutu are mainly due to the excavation of the Ziwei crater. The TiO2 + FeO content map derived by the radar has a much higher spatial resolution compared to orbital observation, testifying the feasibility of this technique for regional geology study.
The Siberian moth (Dendrolimus sibiricus), a pest risk assessment for Norway
Authors: Flø Daniel , Rafoss Trond , Wendell Michael , Sundheim Leif 
Published: July 2020

Abstract
Background
The Siberian moth (Dendrolimus sibiricus) is a serious pest of conifers in Russia, Northern Kazakhstan, Mongolia and China. The western border of the pest’s distribution in Russia is disputed, but it is present west of the 60th meridian east. The pest has the potential to defoliate a wide range of conifers.

Results
The pest is not present in Norway or other European countries, except Russia. Natural spread and human mediated transport are potential pathways for the pest. Human mediated pathways considered are: Living trees for planting, coniferous wood in the rough and foliage and branches. There has been no import of living trees from Russia to Norway during the past 30 years, and there is currently no import of coniferous wood commodities containing bark from areas, where D. sibiricus occurs.

Conclusions
The probability of D. sibiricus entry by natural spread is unlikely, mainly because of the geographical distance and the partial sea barriers between Norway and the infested areas. The probability of entry by human mediated pathways is unlikely due to the very limited volume of the import. Should the pest enter Norway, the probability of establishment and spread is unlikely, due to the suboptimal climatic conditions, and the fact that the two dominant conifers in the country, Norway spruce (Picea abies) and Scots pine (Pinus sylvestris), are intermediate and poor hosts, respectively. The potential damage, should D. sibiricus enter Norway, is considered low.