bluuebunny/crossref_metadata_2025_split huggingface dataset:
MCTR1 alleviates lipopolysaccharide‐induced acute lung injury by protecting lung endothelial glycocalyx
Authors: Li Hui , Hao Yu , Yang Li‐Li , Wang Xin‐Yang , Li Xin‐Yu , Bhandari Suwas , Han Jun , Liu Yong‐Jian , Gong Yu‐Qiang , Scott Aaron , Smith Fang Gao , Jin Sheng‐Wei 
Published: February 2020

AbstractEndothelial glycocalyx degradation, critical for increased pulmonary vascular permeability, is thought to facilitate the development of sepsis into the multiple organ failure. Maresin conjugates in tissue regeneration 1 (MCTR1), a macrophage‐derived lipid mediator, which exhibits potentially beneficial effects via the regulation of bacterial phagocytosis, promotion of inflammation resolution, and regeneration of tissue. In this study, we show that MCTR1 (100 ng/mouse) enhances the survival of mice with lipopolysaccharide (LPS)‐induced (15 mg/kg) sepsis. MCTR1 alleviates LPS (10 mg/kg)‐induced lung dysfunction and lung tissue inflammatory response by decreasing inflammatory cytokines (tumor necrosis factor‐α, interleukin‐1β [IL‐1β], and IL‐6) expression in serum and reducing the serum levels of heparan sulfate (HS) and syndecan‐1. In human umbilical vein endothelial cells (HUVECs) experiments, MCTR1 (100 nM) was added to the culture medium with LPS for 6 hr. MCTR1 treatment markedly inhibited HS degradation by downregulating heparanase (HPA) protein expression in vivo and in vitro. Further analyses indicated that MCTR1 upregulates sirtuin 1 (SIRT1) expression and decreases NF‐κB p65 phosphorylation. In the presence of BOC‐2 or EX527, the above effects of MCTR1 were abolished. These results suggest that MCTR1 protects against LPS‐induced sepsis in mice by attenuating pulmonary endothelial glycocalyx injury via the ALX/SIRT1/NF‐κB/HPA pathway.
A novel role of SIRT2 in regulating gap junction communications via connexin‐43 in bovine cumulus‐oocyte complexes
Authors: Xu Dejun , He Huanshan , Liu Dingbang , Geng Guoxia , Li Qingwang 
Published: February 2020

AbstractSIRT2, the predominantly cytosolic sirtuin, plays important role in multiple biological processes, including metabolism, stress response, and aging. However, the function of SIRT2 in gap junction intercellular communications (GJICs) of cumulus‐oocyte complexes (COCs) is not yet known. The purpose of the present study was to evaluate the effect and underlining mechanism of SIRT2 on GJICs in COCs. Here, we found that treatment with SIRT2 inhibitors (SirReal2 or TM) inhibited bovine oocyte nuclear maturation. Further analysis revealed that SIRT2 inactivation disturbed the GJICs of COCs during in vitro maturation. Correspondingly, both the Cx43 phosphorylation levels and MEK/MER signaling pathways were induced by SIRT2 inhibition. Importantly, SIRT2‐mediated Cx43 phosphorylation was completely abolished by treatment with MEK1/2 inhibitor (Trametinib). Furthermore, treatment with SIRT2 inhibitors resulted in the high levels of MEK1/2 acetylation. Functionally, downregulating the MER/ERK pathways with inhibitors (Trametinib or SCH772984) could attenuate the closure of GJICs caused by SIRT2 inactivation in partly. In addition, inhibition of SIRT2 activity significantly decreased the membrane and zona pellucida localization of Cx43 by upregulating the levels of Cx43 acetylation. Taken together, these results demonstrated a novel role that SIRT2 regulates GJICs via modulating the phosphorylation and deacetylation of Cx43 in COCs.
Antagonistic relationship between the unfolded protein response and myocardin‐driven transcription in smooth muscle
Authors: Zhu Baoyi , Daoud Fatima , Zeng Shaohua , Matic Ljubica , Hedin Ulf , Uvelius Bengt , Rippe Catarina , Albinsson Sebastian , Swärd Karl 
Published: February 2020

AbstractSmooth muscle cells (SMCs) are characterized by a high degree of phenotypic plasticity. Contractile differentiation is governed by myocardin‐related transcription factors (MRTFs), in particular myocardin (MYOCD), and when their drive is lost, the cells become proliferative and synthetic with an expanded endoplasmic reticulum (ER). ER is responsible for assembly and folding of secreted proteins. When the load on the ER surpasses its capacity, three stress sensors (activating transcription factor 6 [ATF6], inositol‐requiring enzyme 1α [IRE1α]/X‐box binding protein 1 [XBP1], and PERK/ATF4) are activated to expand the ER and increase its folding capacity. This is referred to as the unfolded protein response (UPR). Here, we hypothesized that there is a reciprocal relationship between SMC differentiation and the UPR. Tight negative correlations between SMC markers (MYH11, MYOCD, KCNMB1, SYNPO2) and UPR markers (SDF2L1, CALR, MANF, PDIA4) were seen in microarray data sets from carotid arterial injury, partial bladder outlet obstruction, and bladder denervation, respectively. The UPR activators dithiothreitol (DTT) and tunicamycin (TN) activated the UPR and reduced MYOCD along with SMC markers in vitro. The IRE1α inhibitor 4μ8C counteracted the effect of DTT and TN on SMC markers and MYOCD expression. Transfection of active XBP1s was sufficient to reduce both MYOCD and the SMC markers. MRTFs also antagonized the UPR as indicated by reduced TN and DTT‐mediated induction of CRELD2, MANF, PDIA4, and SDF2L1 following overexpression of MRTFs. The latter effect did not involve the newly identified MYOCD/SRF target MSRB3, or reduced production of either XBP1s or cleaved ATF6. The UPR thus counteracts SMC differentiation via the IRE1α/XBP1 arm of the UPR and MYOCD repression.
Two insulin‐like peptides may regulate egg production in opposite directions via juvenile hormone signaling in the queenless ant Pristomyrmex punctatus
Authors: Araki Marina , Miyakawa Misato O. , Suzuki Tomohiro , Miyakawa Hitoshi 
Published: February 2020

AbstractIn the major eusocial species of Hymenoptera, the regulatory mechanisms controlling queen/worker differentiation and exclusive reproduction by queens have been studied extensively. These studies have shown that insulin/insulin‐like growth factors and juvenile hormones (JHs) act as key endocrine factors. However, although considerable knowledge has accumulated in this area, large disparities in the regulatory mechanisms governing caste differentiation have been observed in different hymenopteran taxa to date. We focused on the queenless ant Pristomyrmex punctatus (Hymenoptera: Formicidae), which exhibits the simplest type of sociality and in which reproductive tasks (egg production) are distributed among morphologically and genetically identical workers. To elucidate the molecular mechanisms underlying reproduction in P. punctatus, we analyzed the correlations between the gene expression profiles of a reproductive marker gene, vitellogenin (PripuVTG1), and candidate regulatory genes comprising the major components of the JH and insulin/insulin‐like growth factor signaling pathways that are involved in the regulation of reproduction upstream of JH signaling. Expression of insulin‐like peptide 1 (PripuILP1) and JH signaling‐related genes was negatively correlated with PripuVTG1 expression. On the contrary, insulin‐like peptide 2 (PripuILP2a) was positively correlated with PripuVTG1. These findings suggest that an equilibrium perhaps controlled by switches in JH signaling exists between these two ILP paralogs, and that these interactions are important for regulating reproduction. Our findings are expected to be useful for understanding how various modes of sociality have evolved in insects.
Updated Australian diagnostic reference levels for adult CT
Authors: Lee Kam L. , Beveridge Toby , Sanagou Masoumeh , Thomas Peter 
Published: February 2020

AbstractIntroductionIn 2018, ARPANSA published updated national DRLs for adult CT, which were first published in 2012, and augmented the national DRL categories. This paper presents the updated national DRLs and describes the process by which they were produced.MethodsExamine patient survey data submitted to the Australian Radiation Protection and Nuclear Safety Agency (ARPANSA) National Diagnostic Reference Level Service (NDRLS). Determine the quartiles of the distributions of median survey dose metrics with categorisation by procedure type. Engage a liaison panel representing the radiology professions to review procedure categories and recommend revised national DRLs. The revised NDRL procedure categories are: head (non‐contrast brain (trauma/headache)), cervical spine (Non‐contrast (trauma)), soft‐tissue neck (post‐contrast (oncology)), chest (post‐contrast (oncology)), abdomen–pelvis (post‐contrast (oncology)), kidney–ureter–bladder (non‐contrast (suspected renal colic)), chest–abdomen–pelvis (post‐contrast (oncology)) and lumbar spine (non‐contrast (degenerative pain)).ResultsThe existing six procedure categories were revised and refined. Updated Australian national diagnostic reference levels for adult CT were recommended and endorsed for eight procedure categories: head (52 mGy/880 mGycm), cervical spine (23 mGy/470 mGycm),soft‐tissue neck (17 mGy/450 mGycm), chest (10 mGy/390 mGycm), abdomen–pelvis (13 mGy/600 mGycm), kidney–ureter–bladder (13 mGy/600 mGycm), chest–abdomen–pelvis (11 mGy/940 mGycm) and lumbar spine (26 mGy/670 mGycm). The updated national DRLs are between 12 and 26% lower than the previous DRLs for dose‐length product and between 13 and 63% lower for volume computed tomography dose index.ConclusionsAustralian national DRLs for adult CT have been reviewed and revised. The revised national DRLs are lower, better reflecting current practice among imaging facilities in Australia. The revised Australian national DRLs are similar to those in other developed countries.
Differential Resource Use between Native and Introduced Gray Squirrels
Authors: Johnston Aaron N. , Vander Haegen W. Matthew , West Stephen D. 
Published: February 2020

ABSTRACTDifferences in habitat requirements and the spatial distribution of habitat for native and introduced species can determine outcomes of biological invasions. Introduced eastern gray squirrels (Sciurus carolinensis) have displaced native red squirrels (S. vulgaris) in Europe and have been implicated as a contributing factor to the decline of western gray squirrels (S. griseus) in North America. Eastern and western gray squirrels are associated with oak (Quercus spp.), but little is known about how these species interact. From April 2007 to April 2012, we radio‐tracked sympatric eastern and western gray squirrels in western Washington to compare habitat use and evaluate competitive interactions. We developed resource utilization functions for each species and evaluated distribution of habitat on Joint Base Lewis‐McChord, Washington, USA. Both species shared affinity for closed canopy forests and oaks; however, important differences in relationships with riparian areas and shrub cover resulted in low overlap in habitat distribution, which likely limited potential competitive interactions. Eastern gray squirrels appeared restricted to areas around wetlands likely because they supported the deciduous tree species that comprise habitat for this species in its native range. Use by western gray squirrels, but not eastern gray squirrels, significantly decreased with increasing shrub cover. Forestry practices that promote mixed oak‐conifer with little shrub cover in uplands can benefit western gray squirrels and minimize interactions with eastern gray squirrels. © 2020 The Wildlife Society.
The Relationship between Croup and Gastroesophageal Reflux: A Systematic Review and Meta‐Analysis
Authors: Coughran Alanna , Balakrishnan Karthik , Ma Yifei , Vaezeafshar Reza , Capdarest‐Arest Nicole , Hamdi Osama , Sidell Douglas R. 
Published: February 2020

ObjectiveThe mechanism by which recurrent croup occurs is unknown. Gastroesophageal reflux is commonly implicated, although this relationship is only loosely documented. We conducted a systematic review with a meta‐analysis component to evaluate the relationship between recurrent croup and gastroesophageal reflux disease (GERD), and to assess for evidence of improvement in croup symptoms when treated.Style DesignSystematic Review and Meta Analysis.MethodsWe searched five separate databases. Studies were included if they discussed the relationship between croup and GERD in children, >5 subjects, and available in English. Literature retrieved was assessed according to pre‐specified criteria. Retrieved articles were reviewed by two independent authors and decisions mediated by a third author. If there was a difference of opinion after first review, a second review was performed to obtain consensus. Heterogeneity was calculated and summarized in forest plots.ResultsOf 346 initial records, 15 met inclusion criteria. These were two retrospective cohort and 13 cross‐sectional studies. Thirteen of 15 articles support an association between recurrent croup and GERD. Although heterogeneity is high among studies that reported prevalence of GERD, there is less uncertainty in results for improvement to recurrent croup after GERD treatment. Most studies lacked a control group and all carry a moderate‐to‐high risk of bias.ConclusionThere is limited evidence linking GERD to recurrent croup; Further research is needed to assess for causality as most studies are retrospective, lack a control group, and have a study design exposing them to bias. Patients treated with reflux medication appear to demonstrate a reduced incidence of croup symptoms.Level of Evidence1 Laryngoscope, 131:209–217, 2021
No Shortage of Decibels in Music City: Evaluation of Noise Exposure in Urban Music Venues
Authors: Tittman Sarah M. , Yawn Robert J. , Manzoor Nauman , Dedmon Matthew M. , Haynes David S. , Rivas Alejandro 
Published: February 2020

Objectives/HypothesisTo measure and report noise exposure at urban music venues and assess the risk of noise‐induced hearing loss by comparing these measures to the National Institute of Occupations Safety and Health (NIOSH) guidelines.Study DesignObservational study.MethodsA commercially available smartphone and external calibrated microphone were used to measure sound levels at urban music venues. The maximum sound level, equivalent continuous sound level, and statistical noise levels (L10, L50, L75, L90) were recorded.ResultsThe average equivalent continuous sound level was 112.0 (±4.9) dBA, and the average maximum sound level was 127.0 (±3.2) dBA. The L90 average (sound levels at or above this loudness for 90% of measured exposure time) was 101.1 (±5.5) dBA, and the L10 average was 115.2 (±5.0) dBA. Based off of NIOSH guidelines, noise exposure duration at the L10 average should not exceed 28 seconds, and those at the L90 average should not exceed 12 minutes.ConclusionsSmartphone applications using external calibrated microphones can provide useful sound measurements. Data show that randomly sampled music venues may have noise levels that place patrons without hearing protection at risk for noise‐induced hearing loss with prolonged exposure.Level of EvidenceNA Laryngoscope, 131:25–27, 2021
Gut microbial fatty acid metabolites (KetoA and KetoC) affect the progression of nonalcoholic steatohepatitis and reverse cholesterol transport metabolism in mouse model
Authors: Sofyana Neng Tanty , Zheng Jiawen , Manabe Yuki , Yamamoto Yuta , Kishino Shigenobu , Ogawa Jun , Sugawara Tatsuya 
Published: February 2020

AbstractNonalcoholic steatohepatitis (NASH) is a common liver disease that occurs in both alcoholics and nonalcoholics. Oxidative stress is a possible causative factor for liver diseases including NASH. Gut microorganisms, especially lactic acid bacteria, can produce unique fatty acids, including hydroxy, oxo, conjugated, and partially saturated fatty acids. The oxo fatty acid 10‐oxo‐11(E)‐octadecenoic acid (KetoC) provides potent cytoprotective effects against oxidative stress through activation of Nrf2‐ARE pathway. The aim of this study was to explore the preventive and therapeutic effects of gut microbial fatty acid metabolites in a NASH mouse model. The mice were divided into 3 experimental groups and fed as follows: (1) high‐fat diet (HFD) (2) HFD mixed with 0.1% KetoA (10‐oxo‐12(Z)‐octadecenoic acid), and (3) HFD mixed with 0.1% KetoC. After 3 weeks of feeding, plasma parameters, liver histology, and mRNA expression of multiple genes were assessed. There was hardly any difference in fat accumulation in the histological study; however, no ballooning occurred in 2/5 mice of KetoC group. Bridging fibrosis was not observed in the KetoA group, although KetoA administration did not significantly suppress fibrosis score (p = 0.10). In addition, KetoC increased the expression level of HDL related genes and HDL cholesterol levels in the plasma. These results indicated that KetoA and KetoC may partly affect the progression of NASH in mice models.
Tying Polarization‐Switchable Optical Vortex Knots and Links via Holographic All‐Dielectric Metasurfaces
Authors: Guo Xuyue , Li Peng , Zhong Jinzhan , Liu Sheng , Wei Bingyan , Zhu Wei , Qi Shuxia , Cheng Huachao , Zhao Jianlin 
Published: February 2020

AbstractTailoring the spatial structure of light field in multiple degrees of freedom is a research hotspot in recent years. The topology of light field, as one of the most fascinating structures, such as vortex knots and links associated with the phase singularities, is evoking increasing attention both in fundamental research and practical application. Here, an all‐dielectric metasurface device is proposed and experimentally demonstrated that can construct vortex knots and links in light field at the micro scale. These two distinct topological configurations can be switched by changing the incident polarization. Utilizing the digital holographic interference method, these topological configurations of vorticity lines are accurately characterized in three‐dimensions, and the topology‐preserving evolution of such ultra‐small fields is demonstrated. These two classical configurations exemplify the capability of multichannel manipulating topology by compact metadevice. The work may promote the application of structured light filed at the micro scale and even the creation of other physical fields with ultra‐small size.
Inhibition effect and theoretical investigation of dicarboxylic acid derivatives as corrosion inhibitor for aluminium alloy
Authors: Zhang Daquan , Yang Hanxue , Li Xiaohui , Chen ShiLin , Gao Lixin , Lin Tong 
Published: February 2020

AbstractThe corrosion inhibition ability of l‐malic acid (MaA) and l‐aspartate acid (AsA) against corrosion of the AA5052 alloy in 4 M sodium hydroxide–ethylene glycol solution is investigated. The presence of MaA and AsA in corrosion solution shows a remarkable inhibition of hydrogen evolution of the AA5052 alloy. AsA has the better inhibition effect for the self‐corrosion of the AA5052 alloy and its max protection efficiency is 82.1%. This shows that the Mulliken charges of the nitrogen atom of the amine group on the AsA is lower than the Mulliken charge of the oxygen atom of the hydroxyl group on the MaA; the total Mulliken charge of AsA is lower than MaA, and AsA has a higher EHOMO and a lower energy gap, ΔN. The amine group and carboxyl group on the AsA is easier to coordinate with Al3+ ions to form a stable complex.
A Transparent, High Refractive, Shape‐Memory, Healable, and Recyclable Phenolic Polyurethane Thermoset: Unexpected Roles of Bromine Substituents and Tertiary Amine Catalysts
Authors: Bai Lu , Zheng Junping 
Published: February 2020

AbstractThe applications of polyurethane (PU) thermosets are limited by the lack of plasticity after curing due to permanent crosslinks. Besides, the low refractive index is insufficient for optical devices. Here, a transparent, high refractive, recyclable, and healable PU thermoset is developed based on dynamic bromined phenyl‐carbamate bonds (BPCB). It exhibits tensile strength of ≈52 MPa, good shape‐memory ability, and can be reprocessed or healed nearly completely at 110 °C in 30 min. Tertiary amine catalysts and bromine substituents jointly play unexpected roles to promote the forward and reverse reactions significantly, leading to high bond‐exchange efficiency of BPCB. Furthermore, the introducing of BPCB can endow the material with higher refractive index (nD = 1.5850) simultaneously. In summary, this material is of potential as dynamic PU thermoset in various fields, especially in advanced optical devices.
Comprehensive analysis of somatic copy number alterations in clear cell renal cell carcinoma
Authors: Tsuyukubo Takashi , Ishida Kazuyuki , Osakabe Mitsumasa , Shiomi Ei , Kato Renpei , Takata Ryo , Obara Wataru , Sugai Tamotsu 
Published: February 2020

AbstractSomatic copy number alterations (SCNAs) are important biological characteristics that can identify genome‐wide alterations in renal cell carcinoma (RCC). Recent studies have shown that SCNAs have potential value for determining the prognosis of RCC. We examined SCNAs using the Affymetrix platform to analyze samples from 59 patients with clear cell RCCs (ccRCCs) including first cohort (30 cases) and second cohort (validation cohort, 29 cases). We stratified SCNAs in the ccRCCs using a hierarchical cluster analysis based on SCNA types, including gain, loss of heterozygosity (LOH), copy neutral LOH, mosaic, and mixed types. In this way, the examined two cohorts were categorized into two subgroups (1 and 2). Although the frequency of mixed type was higher in subgroup 1 than in subgroup 2 in the two cohorts, the association did not reach statistical significance. There was a significant difference in the frequency of metachronous metastasis between subgroups 1 and 2 (subgroup 2 > 1). In addition, subgroup 2 was retained in multivariate analysis of both cohorts. We examined whether there were specific alleles differing between subgroups 1 and 2 in both cohorts. We found that there was indeed a statistically significant difference in the 3p mixed types. Among the 3p mixed type, we found that 3p24.3 mixed type was inversely correlated with the presence of metachronous metastasis in ccRCC. The association was also retained in multivariate analysis in second cohort. We suggest that the 3p24.3 mixed type may be a novel marker to predict a favorable prognosis in ccRCC.
Spotlighting the hypoxia‐centrosome amplification axis
Authors: Mittal Karuna , Aneja Ritu 
Published: February 2020

AbstractThe abysmal success rate of anticancer drugs in clinical trials, is in part, attributable to discordance between cultured cancer cells and patient tumors. While tumors in vivo, display a lower mitotic index, patient tumors portray much higher centrosomal aberrations, relative to in vitro cultured cells. The microenvironment too differs considerably between the in vitro and in vivo scenarios. Notably, another hallmark of cancer, hypoxia, is not recapitulated in cell lines cultured under normoxic conditions. These observations raise the possibility that hypoxia may be the missing link that explains the discordance between cell biological phenomena in vitro versus physiological conditions. Further, the interplay between hypoxia and centrosome amplification (CA) is relatively understudied. Recent research from our laboratory, geared toward examining the biological link between the two, has uncovered that hypoxia induces the expression of proteins (Plk4, Aurora A, Cyclin D) implicated in CA, in a hypoxia‐inducible factor 1α (HIF‐1α)‐dependent context. Our studies evidence that hypoxia fuels CA that underlie intratumoral heterogeneity and metastatic potential of cancer cells. Given the advent of HIF‐1α inhibitors, this research has ramifications in aiding patient risk stratification and designing new cancer drug therapies to facilitate clinical decision‐making.