bluuebunny/crossref_metadata_2025_split huggingface dataset:
The T-pod is as stable as supraacetabular fixation using 1 or 2 Schanz screws in partially unstable pelvic fractures: a biomechanical study
Authors: Zeckey Christian , Cavalcanti Kußmaul Adrian , Suero Eduardo M. , Kammerlander Christian , Greiner Axel , Woiczinski Matthias , Braun Christian , Flatz Wilhelm , Boecker Wolfgang , Becker Christopher A. 
Published: July 2020

Abstract
Introduction
Unstable fractures of the pelvis remain the predominant cause of severe hemorrhage, shock and early death in severely injured patients. The use of pelvic binders has become increasingly popular, particularly in the preclinical setting. There is currently insufficient evidence available about the stability of the pelvic binder versus supraacetabular fixation using 1 or 2 Schanz screws. We aimed to analyze the stability of the pelvic binder and supraacetabular fixateurs using either 1 or 2 Schanz screws in a cadaver model of an induced pelvic B-type fracture.

Materials and methods
The study was undertaken in 7 human fresh-frozen cadaveric pelvises with induced AO-type B fractures. Three stabilization techniques were compared: T-POD (pelvic bandage), supraacetabular external fixator with 1 pin on each side and external fixator with 2 pins on each side. Stability and stiffness were analyzed in a biomechanical testing machine using a 5-step protocol with static and dynamic loading, dislocation data were retrieved by ultrasound sensors at the fracture sites.

Results
No significant differences in fracture fragment displacement were detected when using either the T-POD, a 1-pin external fixator or a 2-pin external fixator (P > 0.05). The average difference in displacement between the three methods was < 1 mm.

Conclusions
Pelvic binders are suitable for reduction of pelvic B-type fractures. They provide stability comparable to that of supraacetabular fixators, independently of whether 1 or 2 Schanz screws per side are used. Pelvic binders provide sufficient biomechanical stability for transferring patients without the need to first replace them with surgically applied external fixators. However, soft tissue irritation has to be taken into consideration and prolonged wear should be avoided.

Level of evidence
Level III
Fine-tuning the metabolic rewiring and adaptation of translational machinery during an epithelial-mesenchymal transition in breast cancer cells
Authors: Fernández-Calero Tamara , Davyt Marcos , Perelmuter Karen , Chalar Cora , Bampi Giovana , Persson Helena , Tosar Juan Pablo , Hafstað Völundur , Naya Hugo , Rovira Carlos , Bollati-Fogolín Mariela , Ehrlich Ricardo , Flouriot Gilles , Ignatova Zoya , Marín Mónica 
Published: July 2020

Abstract

Background
During breast cancer progression, the epithelial to mesenchymal transition has been associated with metastasis and endocrine therapy resistance; however, the underlying mechanisms remain elusive. To gain insight into this process, we studied the transition undergone by MCF7-derived cells, which is driven by the constitutive nuclear expression of a MKL1 variant devoid of the actin-binding domain (MKL1 ΔN200). We characterized the adaptive changes that occur during the MKL1-induced cellular model and focused on regulation of translation machinery and metabolic adaptation.

Methods
We performed a genome-wide analysis at the transcriptional and translational level using ribosome profiling complemented with RNA-Seq and analyzed the expression of components of the translation machinery and enzymes involved in energy metabolism. NGS data were correlated with metabolomic measurements and quantification of specific mRNAs extracted from polysomes and western blots.

Results
Our results reveal the expression profiles of a luminal to basal-like state in accordance with an epithelial to mesenchymal transition. During the transition, the synthesis of ribosomal proteins and that of many translational factors was upregulated. This overexpression of the translational machinery appears to be regulated at the translational level. Our results indicate an increase of ribosome biogenesis and translation activity. We detected an extensive metabolic rewiring occurring in an already “Warburg-like” context, in which enzyme isoform switches and metabolic shunts indicate a crucial role of HIF-1α along with other master regulatory factors. Furthermore, we detected a decrease in the expression of enzymes involved in ribonucleotide synthesis from the pentose phosphate pathway. During this transition, cells increase in size, downregulate genes associated with proliferation, and strongly upregulate expression of cytoskeletal and extracellular matrix genes.

Conclusions
Our study reveals multiple regulatory events associated with metabolic and translational machinery adaptation during an epithelial mesenchymal-like transition process. During this major cellular transition, cells achieve a new homeostatic state ensuring their survival. This work shows that ribosome profiling complemented with RNA-Seq is a powerful approach to unveil in-depth global adaptive cellular responses and the interconnection among regulatory circuits, which will be helpful for identification of new therapeutic targets.
Treatment quality and outcome for multidrug-resistant tuberculosis patients in four regions of China: a cohort study
Authors: Zheng Xu-Bin , Diwan Vinod K. , Zhao Qi , Hu Yi , Bruchfeld Judith , Jiang Wei-Li , Hoffner Sven , Xu Biao 
Published: July 2020

Abstract
Background
China incurs an extremely low treatment coverage of multidrug-resistant tuberculosis (MDR-TB). This study aimed to understand the experience of MDR-TB patients on quality of health care, and the clinical impact through an up to six-year follow-up.

Methods
Cohorts of MDR-TB patients were built in TB/MDR-TB designated hospitals in four regions of China from 2014 to 2015. Patients were followed up during treatment course, and yearly confirmation afterward until 2019. Delay in MDR-TB diagnosis and treatment was calculated upon bacteriological confirmation and treatment initiation. Risk factors for unfavourable outcomes were identified by multivariate logistic regression.

Results
Among 1168 bacteriological-positive TB patients identified from a 12-million population, 58 (5.0%) MDR-TB cases were detected. The median delay for MDR-TB diagnosis was 90.0 days, with 13.8% having a delay above 180.0 days. MDR-TB treatment was only recommended to 19 (32.8%) participants, while the rest continued with regimen for drug-susceptible TB. In MDR-TB treatment group, 36.8% achieved treatment success, while the others had incomplete treatment (21.1%), loss to follow-up (36.8%) and TB relapse (5.3%). For non-MDR-TB treatment group, 33.3% succeeded, 25.6% relapsed, 2.6% failed, 23.1% died, and 15.4% were lost to follow-up. Overall, only 35.7% (20/56) of detected MDR-TB patients had favourable outcomes and higher education level was positively associated with it (adjusted odds ratio [aOR]: 3.60, 95% confidence interval [CI]: 1.04–12.5).

Conclusions
A large proportion of patients did not receive MDR-TB treatment and had unfavourable outcomes. Delayed MDR-TB diagnosis resulted in poor quality of MDR-TB care. Rapid diagnosis, regulated patient management and high-quality MDR-TB treatment should be enhanced in China.
Designing and validating a potential assessment inventory for assessing ELTs’ assessment literacy
Authors: Nikmard Fateme , Mohamadi Zenouzagh Zohre 
Published: July 2020

AbstractEnglish teachers' assessment literacy has always been considered as an important factor in their performance. However, no instrument has ever been developed to assess this construct among Iranian EFL teachers. To fill this gap, in the first phase of the present study a theoretical framework for the main four components of teacher assessment literacy, named validity, reliability, interpretability of the results, and efficiency, was developed through extensive review of the related literature and conducting interviews with PhD candidates of TEFL. In the second phase, a questionnaire was developed and piloted with 150 participants who took part in the study through the rules of convenience sampling. More specifically, the 30 items of the newly-developed “ELTs’ Assessment Literacy” questionnaire were subjected to factor analysis which revealed the presence of all the four components consisting of different number of items. These phases led to the development of a questionnaire with four components and 25 items on the basis of a five point Likert scale that measured: (1) “Validity” including six items, (2) “Reliability” including ten items, (3) “Interpretability of the Results” including eight item, and (4) “Efficiency” including five items. The findings of this study may shed lights on this subject and help researchers and teaching practitioners assess EFL teachers’ assessment literacy and make principled decisions as far as assessment is concerned.
CT features associated with EGFR mutations and ALK positivity in patients with multiple primary lung adenocarcinomas
Authors: Han Xiaoyu , Fan Jun , Gu Jin , Li Yumin , Yang Ming , Liu Tong , Li Nan , Zeng Wenjuan , Shi Heshui 
Published: July 2020

Abstract
Background
In multiple primary lung adenocarcinomas (MPLAs), the relationship between imaging and gene mutations remains unclear. This retrospective study aimed to identify the correlation of epidermal growth factor receptor (EGFR) mutations and anaplastic lymphoma kinase (ALK) status with CT characteristics in MPLA patients.

Methods
Sixty-seven patients (135 lesions) with MPLAs confirmed by pathology were selected from our institution. All subjects were tested for EGFR mutations and ALK status and underwent chest CT prior to any treatment. The criteria for MPLA definitions closely adhered to the comprehensive histologic assessment (CHA).

Results
Among MPLA patients, EGFR mutations were more common in females (p = 0.002), in those who had never smoked (p = 0.010), and in those with less lymph node metastasis (p < 0.001), and the tumours typically presented with ground-glass opacity (GGO) (p = 0.003), especially mixed GGO (p < 0.001), and with air bronchograms (p = 0.012). Logistics regression analysis showed that GGO (OR = 6.550, p = 0.010) was correlated with EGFR mutation, while air bronchograms were not correlated with EGFR mutation (OR = 3.527, p = 0.060). A receiver operating characteristic (ROC) curve yielded area under the curve (AUC) values of 0.647 and 0.712 for clinical-only or combined CT features, respectively, for prediction of EGFR mutations, and a significant difference was found between them (p = 0.0344). ALK-positive status was found most frequently in MPLA patients who were younger (p = 0.002) and had never smoked (p = 0.010). ALK positivity was associated with solid nodules or masses in MPLAs (p < 0.004) on CT scans. Logistics regression analysis showed that solid nodules (OR = 6.550, p = 0.010) were an independent factor predicting ALK positivity in MPLAs. For prediction of ALK positivity, the ROC curve yielded AUC values of 0.767 and 0.804 for clinical-only or combined CT features, respectively, but no significant difference was found between them (p = 0.2267).

Conclusion
Among MPLA patients, nonsmoking women with less lymph node metastasis and patients with lesions presenting GGO or mixed GGO and air bronchograms on CT were more likely to exhibit EGFR mutations. In nonsmoking patients, young patients with solid lesions on CT are recommended to undergo an ALK status test.
Cerebellum and micturition: what do we know? A systematic review
Authors: Bastide Laure , Herbaut Anne-Geneviève 
Published: July 2020

Abstract
Aims
Micturition depends on a complex voluntary and involuntarily neuronal network located at various levels of the nervous system. The mechanism is highly dependent on the hierarchical organization of central nervous system pathways. If the role of the cortex and brainstem centres is well established, the role of other subcortical areas structures, such as the cerebellum is poorly understood. We are interested in discussing the current knowledge on the role of cerebellum in micturition.

Methods
A systematic search is performed in the medical literature, using the PubMed database with the keyword « cerebellum ». The latter is combined with «urination » OR « micturition » OR « urinary bladder ».

Results
Thirty-one articles were selected, focussing on micturition and describing the role of the cerebellum. They were grouped in 6 animal experimental studies, 20 functional brain imaging in micturition and 5 clinical studies.

Conclusions
Although very heterogeneous, experimental and clinical data clearly indicate the cerebellum role in the micturition control. Cerebellum modulates the micturition reflex and participates to the bladder sensory-motor information processing. The cerebellum is involved in the reflex micturition modulation through direct or indirect pathways to major brainstem or forebrain centres.
Goblet cell carcinoid of the rectum: a case report
Authors: Inoue Yoshiyuki , Horie Hisanaga , Homma Yuko , Sadatomo Ai , Tahara Makiko , Koinuma Koji , Yamaguchi Hironori , Mimura Toshiki , Kihara Atsushi , Lefor Alan Kawarai , Sata Naohiro 
Published: July 2020

Abstract
Background
Goblet cell carcinoid (GCC) is a neuroendocrine tumor usually found in the appendix. GCCs exhibit characteristic findings with mixed endocrine-exocrine features such as staining positive for neuroendocrine markers and producing mucin. The primary GCC of the rectum is exceedingly rare.

Case presentation
A 77-year-old Japanese male presented with hematochezia. Anal tenderness and a hard mass in the anal canal were found on the digital rectal examination, and colonoscopy was performed. Colonoscopy showed an irregularly shaped mass in the anal canal. Biopsy showed mixed features including adenocarcinoma in situ, well-differentiated adenocarcinoma, and mucinous carcinoma with invasive proliferation. No metastatic lesions were found on the computed tomography scan. Pelvic magnetic resonance imaging scan showed extramural growth of a tumor on the ventral side of the rectum without invasion to the prostate. Laparoscopic abdominoperineal resection was performed. The final diagnosis was well-differentiated adenocarcinoma in the mucosa and goblet cell carcinoid from the submucosa to the adventitia of the rectum. The patient was discharged from the hospital on postoperative day 16. Six months after resection, a computed tomography scan revealed multiple metastatic lesions in the liver. Several chemotherapy regimens were given, and the patient has stable disease 27 months after surgery.

Conclusion
We present a patient with rectal GCC with metachronous liver metastases. Since GCC grows intramurally and is biologically aggressive compared to typical carcinoid lesions, the disease is usually diagnosed at an advanced stage. The development of optimal adjuvant chemotherapy is needed for those patients.
Primary monophasic synovial sarcoma of the cervical esophagus confirmed by detection of the SS18-SSX2 fusion transcripts: case report and literature review
Authors: Sasaki Ken , Noda Masahiro , Tsuruda Yusuke , Uchikado Yasuto , Omoto Itaru , Kita Yoshiaki , Arigami Takaaki , Mori Shinichiro , Kurahara Hiroshi , Nakajo Akihiro , Higashi Michiyo , Ohtsuka Takao 
Published: July 2020

Abstract
Background
Synovial sarcoma (SS) of the esophagus is extremely rare. Because of the microscopic features of SS, the monophasic type can easily be misdiagnosed as other spindle cell tumors. Here, we present the first case of a primary SS of the esophagus in the presence of SS18-SSX2 fusion transcripts.

Case presentation
A 47-year-old Japanese woman was initially diagnosed with thyroid papillary carcinoma in the left lobe and leiomyoma of the cervical esophagus and subsequently underwent left thyroid lobectomy and enucleation of the esophageal tumor. Four years after the first surgery, the esophageal tumor recurred. Endoscopic biopsy of the tumor revealed atypical cell proliferation with spindle cell features and mitoses. Immunohistochemistry showed focal positivity for bcl-2 and HHF35. Furthermore, the presence of SS18-SSX2 fusion transcripts was confirmed by reverse transcription-polymerase chain reaction analysis, using a paraffin-embedded tumor specimen. Therefore, the tumor was diagnosed as monophasic SS of the cervical esophagus. We re-evaluated the surgical specimen enucleated 3 years previously, which was initially diagnosed as leiomyoma, and the diagnosis of SS was confirmed. The patient underwent cervical esophagectomy with isolated jejunal interposition reconstruction. Three years after the second surgery, SS recurred in the distal anastomotic site between the jejunum and the esophagus, and the patient underwent thoracoscopic esophagectomy with gastric conduit reconstruction. The pathological grade of the lesion worsened with every recurrence.

Conclusions
Monophasic SS can be difficult to discriminate from other spindle cell tumors based on microscopy alone, and molecular analysis could be useful for confirming the precise diagnosis of monophasic SS.
Validation of a German version of the Caregiver Quality of Life Index-Cancer (CQOLC) in a sample of significant others of breast and gynaecologic cancer patients
Authors: Ehmann Anna Tamara , Mahler Cornelia , Klafke Nadja 
Published: July 2020

AbstractThere is no disease-specific instrument to measure the quality of life of significant others of cancer patients in Germany. In this study, we evaluated the reliability and construct validity of a German version of the Caregiver Quality of Life Index-Cancer (CQOLC) in a sample of 212 caregivers of breast and gynaecologic cancer patients. The CQOLC was administered along with the World Health Organization Quality of Life short version (WHOQOL-BREF) to caregivers of patients taking part in a randomized-controlled intervention study. Data of 212 caregivers were gained at the baseline of the study. Internal consistency was determined by Cronbach’s α. Construct validity was examined by conducting a confirmatory factor analysis (CFA) and hypothesis testing. Correlations between change scores with patients’ global health-related quality of life (HRQoL) were calculated for three time points to evaluate the responsiveness. The three subscales “burden”, “disruptiveness”, and “financial concerns” indicate to a good reliability of the instrument (Cronbach’s α ranged between 0.754 and 0.832), while the subscale “positive adaptation” demonstrated low reliability (α = 0.579). A CFA based on data from the whole set of CQOLC items resulted in CFI levels < .90, and a CFA without problematic items resulted in CFI levels also < .90.The construct validity of the CQOLC could be approved by a moderate to high convergence with close variables as the global HRQoL. Mean differences between caregivers of curatively or palliatively treated patients were nonsignificant (p = 0.959) at T1. Correlations for responsiveness were low with correlation coefficients ranging from 0.030 to 0.326. These data indicate that additional research is needed to further verify the validity of the instrument. The German scale of the CQOLC might be appropriate for clinical and research use, if the wording of some items is refined and if content validity is also assessed by caregivers themselves. The assessment of cancer patients’ caregiver’s quality of life can contribute to a better understanding of the effects of patient-oriented interventions including also closely involved next of kin’s around the cancer patients.
Inversion of surface vegetation water content based on GNSS-IR and MODIS data fusion
Authors: Pan Yalong , Ren Chao , Liang Yueji , Zhang Zhigang , Shi Yajie 
Published: July 2020

AbstractObtaining high-precision, long-term sequences of vegetation water content (VWC) is of great significance for assessing surface vegetation growth, soil moisture, and fire risk. In recent years, the global navigation satellite system-interferometric reflection (GNSS-IR) has become a new type of remote sensing technology with low cost, all-weather capability, and a high temporal resolution. It has been widely used in the fields of snow depth, sea level, soil moisture content, and vegetation water content. The normalized microwave reflectance index (NMRI) based on GNSS-IR technology has been proven to be effective in monitoring changes in VWC. This paper considers the advantages and disadvantages of remote sensing technology and GNSS-IR technology in estimating VWC. A point-surface fusion method of GNSS-IR and MODIS data based on the GA–BP neural network is proposed to improve the accuracy of VWC estimation. The vegetation index products (NDVI, GPP, LAI) and the NMRI that unified the temporal and spatial resolution were used as the input and output data of the training model, and the GA–BP neural network was used for training and modeling. Finally, a spatially continuous NMRI product was generated. Taking a particular area of the United States as a research object, experiments show that (1) a neural network can realize the effective fusion of GNSS-IR and MODIS products. By comparing the GA–BP neural network, BP neural network, and multiple linear regression (MLR), the three models fusion effect. The results show that the GA–BP neural network has the best modeling effect, and the r and RMSE between the model estimation result and the reference value are 0.778 and 0.0332, respectively; this network is followed by the BP neural network, in which the r and RMSE are 0.746 and 0.0465, respectively. MLR has the poorest effect, with r and RMSE values of 0.500 and 0.0516, respectively. (2) The spatiotemporal variation in the 16 days/500 m resolution NMRI product obtained by GA–BP neural network fusion is consistent with that in the experimental area. Through the testing of GNSS stations that did not participate in the modeling, the r between the estimated value of the NMRI and the reference value is greater than 0.87, and the RMSE is less than 0.049. Therefore, the method proposed in this paper is optional and effective. The spatially continuous NMRI products obtained by fusion can reflect the changes in VWC in the experimental area more intuitively.