bluuebunny/crossref_metadata_2025_split huggingface dataset:
Poaceae Orthologs of Rice OsSGL, DUF1645 Domain-Containing Genes, Positively Regulate Drought Tolerance, Grain Length and Weight in Rice
Authors: Liu Kai , Li Mingjuan , Zhang Bin , Cui Yanchun , Yin Xuming , Xia Xinjie , wang manling 
Published: November 2020

Abstract
BackgroundGrain yield is a polygenic trait influenced by environmental and genetic interactions at all growth stages of the cereal plant. However, the molecular mechanisms responsible for coordinating the trade-off or cross-talk between these traits remain elusive.ResultsWe characterized the hitherto unknown function of four STRESS_tolerance and GRAIN_LENGTH (OsSGL) Poaceae ortholog genes, all encoding DUF1645 domain-containing proteins, in simultaneous regulation of grain length, grain weight, and drought stress-tolerance in rice. In normal growth conditions, the four ortholog genes were mainly expressed in the developing roots and panicles of the corresponding species. Over-expressing or heterologous high-level expressing Poaceae OsSGL ortholog genes conferred remarkably increased grain length, weight, and seed setting percentage, as well as significantly improved drought-stress tolerance in transgenic rice. Microscopical analysis also showed that the transgene expression promoted cell division and development. RNA-seq and qRT-PCR analyses revealed 73.8% (18,711) overlapped DEGs in all transgenic plants. Moreover, GO and KEGG analyses of different comparisons revealed that the key DEGs participating in drought stress-response belonged to hormone (especially auxin and cytokinin) pathways, and signaling processes were apparently affected in the young panicles. ConclusionTogether, these results suggest the four OsSGL orthologs perform a conserved function in regulating stress-tolerance and cell growth by acting via a hormone biosynthesis and signaling pathway. It may also induce a strategy for tailor-made crop yield improvement.
Molecular Docking, 3D-QSAR, Fingerprint-Based 2D-QSAR, Analysis of Pyrimidine, and Analogs of ALK (Anaplastic Lymphoma Kinase) Inhibitors as an Anticancer Agent
Authors: Yadav Vivek , Tonk Rajiv Kumar , Khatri Ramchander 
Published: November 2020

Background:
ALK inhibitors have become a plausible option for anticancer therapy with
the availability of several FDA-approved molecules and clinical trial candidates. Hence, the design
of new ALK inhibitors using computational molecular docking studies on the existing inhibitors, is
an attractive approach for anticancer drug discovery.

Methods:
We generated six types of independent models through structural based molecular docking
study, three-dimensional quantitative structure-activity relationship (3D-QSAR) study, and 2DQSAR
approaches using different fingerprints, such as dendritic, linear, 2D molprint, and radial.

Results:
Comparison of the generated models showed that the hinge region hydrogen bond interacted
with amino acids ASP1206, MET1199, and LYS1150 in docking analysis and the hydrophobic
interacted with amino acids GLU1210, ARG1209, SER1206, and LYS1205 residues are responsible
for the ALK inhibition. In the 3D-QSAR study, the hydrogen bond donor features of 2,4-
diaryl aminopyrimidine substituents, isopropyl phenyl ring groups in hydrophobic features, and
electron-withdrawing groups matched the generated contour plots. The 2D-QSAR fingerprint studies
indicated that higher potency was associated with the 2-hydroxy-5-isopropyl benzamide functional
group and substituted phenylamine at the second position of the pyrimidine group.

Conclusion:
We conclude that the incorporation of these functional groups in the design of new
molecules may result in more potent ALK inhibitors.
Current Evidence Regarding Low-carb Diets for The Metabolic Control of Type-2 Diabetes
Authors: Sousa Adriana Andrade , Renke Guilherme , Leal Jr Aluysio , Rassi Marcela Mascarenhas Braga , Feuser Cristianne Serafim , Cunha Heber , Silva Flávia Moraes , Arbex Alberto 
Published: November 2020

:
The management of diabetes requires a medical nutritional therapy as an essential part of this treatment. There
should be no "one-size-fits-all" eating pattern for different patient´s profiles with diabetes. It´s clinically complex to
suggest an ideal percentage of calories from carbohydrates, protein and lipids recommended for all patients with diabetes.
Among the eating patterns that have shown beneficial effects on metabolic control of patients with type 2 diabetes is the
Low-Carb diet, since the carbohydrate ingestion is viewed as the most important determinant of postprandial glucose and
insulin response. In this context, theoretically it could make sense to reduce the daily amount of carbohydrates ingested,
willing to achieve lower levels of HbA1c. There could be associated risks to this approach. The adherence to a Low-Carb
Diet is here also discussed. This narrative review works on the current evidence for answering these questions regarding
Low-Carb Diet as a possible alternative eating pattern for type 2 diabetes.
Formulation Development and Evaluation of Novel Vesicular Carrier for Enhancement of Bioavailability of Poorly Soluble Drug
Authors: Sharma Sumit , Bhatt Shailendra , Saini Vipin 
Published: November 2020

Background::
Niosomes are a vesicular carrier system comprised of a Nonionic surfactant
bilayer surrounding an aqueous compartment. Niosomes are presumed to raise the intake of
the poorly water-soluble drugs by M cells of Peyer's patches present in the intestine's lymphatic tissues,
thereby avoiding the first-pass metabolism and increasing its oral bioavailability. Biodegradability,
nonimmunogenic nature, minimal side effects, low cost, good stability, and flexibility to incorporate
hydrophilic and lipophilic drugs are other advantages of niosomes.

Objective::
To formulate and evaluate a novel vesicular carrier system of a poorly soluble drug
Lurasidone hydrochloride for the enhancement of its solubility and bioavailability.

Methods::
The thin-film hydration technique used to prepare Lurasidone hydrochloride loaded niosomes
using different grades of nonionic surfactants like Brij, Span, and Tween. They evaluated
for particle size, zeta potential, percent entrapment efficiency, in-vitro drug release, and in-vivo
study.

Results::
Niosomes comprised of Brij S-100 in drug: cholesterol: surfactant (1:1:1) showed particle
size (1.15 ± 0.21 μm) and percent entrapment efficiency (97.02 ± 0.21%) and was selected for further
studies. Various pharmacokinetic parameters like Cmax (281.27ng/ml), Tmax (5 h), and AUC
(2640.197) were found to be significantly improved compared to plain drug solution.

Conclusion::
The Niosomal formulation could be the promising drug delivery system for the controlled
and sustained release of Lurasidone.
Feasibility of Bentonite as an Internal Curing Agent in Concrete
Authors: Singh Abhishek , Phaneendra Ch. L. , Saiveer G.S. , Chandravanshi Saurabh , Sharma Udit , Deo Shirish V. 
Published: November 2020

Background:
There is a requirement for internal curing, especially in areas where water is
scarce and where it is difficult to cure concrete due to inaccessibility of the site. About five times higher
water is consumed in curing than the water used in making concrete. Furthermore, the quality of water
that is to be used for these activities is required to be of a certain standard. If the requirement of water
can be reduced, it becomes both economically and ecologically viable.

Objective:
The present research is carried out to determine the technical and economic feasibility of
bentonite as an internal curing agent with properties of concrete to be used in the field.

Methods:
This paper compares the compressive and flexural strength parameters for standard and bentonite
infused concrete and whether this can be considered as a suitable addition in concrete. The flexural
strength is measured using four-point test. Both the tests are carried out in accordance with IS codes.

Results:
There is an increase in compressive strength for both intermittent and air curing of internally
cured concrete in comparison to control concrete. Furthermore, air curing of internally cured concrete is
comparable to intermittent curing of control concrete. Also, the compressive strength of intermittent curing
concrete with bentonite is comparable with the strength obtained by pond curing of control concrete.

Conclusion:
The results indicate that bentonite can be considered as a viable addition to concrete, especially
in cases of air curing. The use of bentonite is thus recommended for air curing and intermittent
curing conditions.
Innovation in Pain Rehabilitation Using Co-Design Methods During the Development of a Relapse Prevention Intervention: Case Study
Authors: Elbers Stefan , van Gessel Christa , Renes Reint Jan , van der Lugt Remko , Wittink Harriët , Hermsen Sander 
Published: November 2020

BackgroundMany intervention development projects fail to bridge the gap from basic research to clinical practice. Instead of theory-based approaches to intervention development, co-design prioritizes the end users’ perspective as well as continuous collaboration between stakeholders, designers, and researchers throughout the project. This alternative approach to the development of interventions is expected to promote the adaptation to existing treatment activities and to be responsive to the requirements of end users.ObjectiveThe first objective was to provide an overview of all activities that were employed during the course of a research project to develop a relapse prevention intervention for interdisciplinary pain treatment programs. The second objective was to examine how co-design may contribute to stakeholder involvement, generation of relevant insights and ideas, and incorporation of stakeholder input into the intervention design.MethodsWe performed an embedded single case study and used the double diamond model to describe the process of intervention development. Using all available data sources, we also performed deductive content analysis to reflect on this process.ResultsBy critically reviewing the value and function of a co-design project with respect to idea generation, stakeholder involvement, and incorporation of stakeholder input into the intervention design, we demonstrated how co-design shaped the transition from ideas, via concepts, to a prototype for a relapse prevention intervention.ConclusionsStructural use of co-design throughout the project resulted in many different participating stakeholders and stimulating design activities. As a consequence, the majority of the components of the final prototype can be traced back to the information that stakeholders provided during the project. Although this illustrates how co-design facilitates the integration of contextual information into the intervention design, further experimental testing is required to evaluate to what extent this approach ultimately leads to improved usability as well as patient outcomes in the context of clinical practice.
The Influence of Average Temperature and Relative Humidity on New Cases of COVID-19: Time-Series Analysis
Authors: He Zonglin , Chin Yiqiao , Yu Shinning , Huang Jian , Zhang Casper J P , Zhu Ke , Azarakhsh Nima , Sheng Jie , He Yi , Jayavanth Pallavi , Liu Qian , Akinwunmi Babatunde O , Ming Wai-Kit 
Published: November 2020

BackgroundThe influence of meteorological factors on the transmission and spread of COVID-19 is of interest and has not been investigated.ObjectiveThis study aimed to investigate the associations between meteorological factors and the daily number of new cases of COVID-19 in 9 Asian cities.MethodsPearson correlation and generalized additive modeling (GAM) were performed to assess the relationships between daily new COVID-19 cases and meteorological factors (daily average temperature and relative humidity) with the most updated data currently available.ResultsThe Pearson correlation showed that daily new confirmed cases of COVID-19 were more correlated with the average temperature than with relative humidity. Daily new confirmed cases were negatively correlated with the average temperature in Beijing (r=–0.565, P<.001), Shanghai (r=–0.47, P<.001), and Guangzhou (r=–0.53, P<.001). In Japan, however, a positive correlation was observed (r=0.416, P<.001). In most of the cities (Shanghai, Guangzhou, Hong Kong, Seoul, Tokyo, and Kuala Lumpur), GAM analysis showed the number of daily new confirmed cases to be positively associated with both average temperature and relative humidity, especially using lagged 3D modeling where the positive influence of temperature on daily new confirmed cases was discerned in 5 cities (exceptions: Beijing, Wuhan, Korea, and Malaysia). Moreover, the sensitivity analysis showed, by incorporating the city grade and public health measures into the model, that higher temperatures can increase daily new case numbers (beta=0.073, Z=11.594, P<.001) in the lagged 3-day model.ConclusionsThe findings suggest that increased temperature yield increases in daily new cases of COVID-19. Hence, large-scale public health measures and expanded regional research are still required until a vaccine becomes widely available and herd immunity is established.
PELAYANAN MASYARAKAT DI BIDANG PERALATAN OLAHRAGA ANAK UNTUK KELOMPOK KERJA GURU PENDIDIKAN FISIK GURU SD DI KOTA TEBING TINGGI TAHUN 2020
Authors: Akhmad Imran , Dewi Rahma , Supriadi Amir 
Published: November 2020

Mitra kegiatan Program Kemitraan Masyarakat adalah kelompok kerja guru pendidikan jasmani kota Tebing Tinggi yang berjarak 60 km dari Universitas Negeri Medan. Guru Pendidikan Jasmani Olahraga Kesehatan tingkat Sekolah Dasar di Kota Tebing Tinggi memiliki beberapa permasalahan proses pembelajaran sehingga kecenderungan mutu pempelajaran Pendidikan Jasmani Olahraga Kesehatan belum maksimal. Adapun permasalahan mitra yang dihadapi selama ini adalah : (1) Kurangnya pengetahuan dan keterampilan Kelompok Kerja Guru Pendidikan Jasmani Olahraga Kesehatan Sekolah Dasar dalam memodifikasi Peralatan Olahraga bagi siswa dan atletik kids, penggunaannya dalam permainan dan analisis data hasil permainan, dan (2) Belum tersedia Peralatan Olahraga disekolah-sekolah di kecamatan mitra yang dapat digunakan dalam rangka membantu proses pembelajaran Pendidikan Jasmai Sekolah Dasar. Kedua permasalahan tersebut menjadi dasar bahwa pembelajaran Pendidikan Jasmani Olahraga Kesehatan belum optimal. Metode yang akan digunakan pada kegiatan ini adalah adalah Pendampingan dan pemberdayaan. Adapun tahapan kegiatan sebagai berikut : 1) Penyediaan Permainan Olahraga Anak, (2) Pelatihan Pelaksanaan Atletik Kids dan teknik penilaian (3) Pelatihan Modifikasi Permainan Olahraga Anak, (4) Pelatihan penggunaan modifikasi Permainan Olahraga Anak dan (5) Perlombaan Atletik Kids. Hasil kegiatan : (1) Permainan Olahraga Anak telah diterima oleh ketua Kelompok Kerja Guru Pendidikan Jasmani Olahraga Kesehatan Sekolah Dasar Kota Tebing Tinggi, (2) Ketercapaian pelatihan penggunaan Permainan Olahraga Anak 92,5%, (3) Ketercapaian indikator penilaian Permainan Olahraga Anak sebesar 89,5%, dan (4) Ketercapaian modifikasi Permainan Olahraga Anak 93%. Program kegiatan Pengabdian Kepada Masyarakat ini menyimpulkan bahwa ; (1) seluruh target capaian terpenuhi, (2) tingkat pengetahuan dan keterampilan modifikasi Permainan Olahraga Anak 90%, (3) dan tingkat kepuasan pelaksanaan kegiatan sebesar 88,5%. Keywords: Modification, Elementry School, Athletic Kids
Memahami perilaku kemandirian belajar Siswa melalui perspektif Human Agency: Sintesis perspektif Human Agency
Authors: Alfaiz Alfaiz , Kadafi Asroful , Yuzarion Yuzarion , Aulia Rahmadianti , Suarja Septya , Mulyani Rila Rahma , Chandra Yasrial , Adison Joni 
Published: November 2020

Human agency adalah konsep bahwa seorang individu memiliki kompetensi dalam perencanaan, disiplin, realisasi dan mengevaluasi perilaku mereka sendiri dalam keadaan hidup termasuk dalam pembelajaran. Ini telah dipelajari dalam pendidikan dengan empat sifat inti seperti sengaja, pemikiran, reaktivitas diri, dan reflektifitas diri membentuk individu sebagai aktor, bukan reaktor. Hal ini dapat digunakan untuk memahami pembelajaran mandiri siswa, karena konsep pembelajaran mandiri memiliki kesadaran diri secara sengaja. Jika individu selalu bergantung pada lingkungannya, itu karena ia tidak memiliki agen dalam keadaan hidupnya. Menurut sebuah penelitian terbaru yang dilakukan pada tahun 2012, telah menemukan bahwa seorang siswa memiliki kurang otonom dalam belajar, dan juga dari survei yang dilakukan pada 2017-2019 ditemukan bahwa 61,50% siswa di 4 sekolah menengah atas memiliki pembelajaran mandiri yang lebih rendah. Fenomena ini mempengaruhi perilaku selingkuh dan menunda-nunda mereka. Pada artikel ini akan membahas kondisi siswa dalam belajar dan memberikan rekomendasi baru dalam perspektif tentang intervensi alternatif dalam praktik bimbingan dan konseling tentang agensi manusia untuk membentuk dan memahami perilaku belajar mandiri dan juga sifat internalisasi agensi manusia dalam proses konseling dalam konteks dalam belajar.Abstract: Human agency is a concept that an individual has a competencies in planning, discipline, realization and evaluate their own behavior in life circumstanceÂ including in learning. It has been studied in education with four core properties such intentionally, forethought, self-reactiveness, and self-reflectivenessÂ shape an individual as an actor, notÂ a reactor. It can be usedÂ to understanding a student autonomous learning, because the concept of autonomous learningÂ has aÂ self-cognition purposely. If individual always dependsÂ on their environment, that because he does not haveÂ an agentic in his life circumstance. According a latestÂ research that conductsÂ in 2012, has found that a studentÂ has a lack of autonomous in learning, and also from survey that conduct in 2017-2019 itâ€™s found that 61,50% students in 4 senior high school has lower autonomous learning. This phenomena influence to their cheating and procrastination behavior. On this article will discuss a studentâ€™s condition in learning and gives a new recommendation in perspectiveÂ about alternative intervention in guidance and counseling practice about the humanÂ agency to shape and understanding an autonomous learning behaviorÂ and also internalization properties of human agency in counseling process in the contextÂ in learning.
Transcriptional profiling of iPSC-derived neurons with reciprocal monogenic CNV in 3p26.3 region
Authors: Лопаткина М.Е. , Фишман В.С. , Гридина М.М. , Скрябин Н.А. , Никитина Т.В. , Кашеварова А.А. , Назаренко Л.П. , Серов О.Л. , Лебедев И.Н. 
Published: November 2020

Проведен анализ генной экспрессии в нейронах, дифференцированных из индуцированных плюрипотентных стволовых клеток пациентов с идиопатическими интеллектуальными нарушениями и реципрокными хромосомными мутациями в регионе 3p26.3, затрагивающими единственный ген CNTN6. Для нейронов с различным типом хромосомных аберраций была показана глобальная дисрегуляция генной экспрессии. В нейронах с вариациями числа копий гена CNTN6 была снижена экспрессия генов, продукты которых вовлечены в процессы развития центральной нервной системы.
The gene expression analysis of iPSC-derived neurons, obtained from patients with idiopathic intellectual disability and reciprocal microdeletion and microduplication in 3p26.3 region affecting the single CNTN6 gene was performed. The global gene expression dysregulation was demonstrated for cells with CNTN6 copy number variation. Gene expression in neurons with CNTN6 copy number changes was downregulated for genes, whose products are involved in the central nervous system development.
Meiotic nonhomologous coorientation in man
Authors: Ковалева Н.В. 
Published: November 2020

Концепция мейотической коориентаци негомологичных хромосом (МКНХ) как модели мейоза, экспериментально подтвержденная на Drosophila, была предложена в 1971 г. для объяснения интерхромосомного эффекта у человека, но с тех пор не получила достаточных доказательств. Представленные в настоящей статье данные «неканонического» поведения половых хромосом в определенных ситуациях являются основанием для признания существования этого явления в сперматогенезе человека.
The concept of meiotic co-orientation of non-homologous chromosomes as a model of meiosis, experimentally confirmed in Drosophila, was proposed in 1971 to explain the interchromosomal effect in humans, but has not received sufficient evidence since. The data of the “non-canonical” behavior of sex chromosomes presented in this article are the basis for recognizing the existence of this phenomenon in human spermatogenesis.
Discordance of karyotypes in chorionic villus cells by autosome aneuploidy
Authors: Головатая Е.И. 
Published: November 2020

Представлены исходы беременностей и результаты цитогенетических исследований 50 случаев дискордантности кариотипов по трисомиям аутосом при получении нормального кариотипа в клетках трофобласта или мезенхимальной стромы ворсин.
Pregnancy outcomes and results of cytogenetic studies of 50 cases of autosome trisomy karyotype discordance in obtaining a normal karyotype in trophoblast cells or villi mesenchymal stroma are presented.
Prenatal diagnosis of oral-facial-digital type I syndrome in a fetus with partial monosomy of Xp22.2
Authors: Минайчева Л.И. , Сеитова Г.Н. , Филиппова М.О. , Кашеварова А.А. , Скрябин Н.А. , Лопаткина М.Е. , Яковлева Ю.С. , Назаренко Л.П. , Лебедев И.Н. 
Published: November 2020

Представлен первый случай пренатальной диагностики оро-фацио-дигитального синдрома I типа (OMIM 311200) у плода с частичной моносомией Хр22.2. Причиной оро-фацио-дигитального синдрома I типа являются мутации в гене OFDI, случаи заболевания вследствие хромосомных микроделеций, затрагивающих ген OFDI встречаются редко. При ультразвуковом исследовании плода во втором триместре беременности выявлены врожденный порок развития головного мозга и множественные эхографические маркеры хромосомной и синдромальной патологии. Биологический образец плода (пуповинная кровь) получен в 21 неделю гестации посредством кордоцентеза. Молекулярно-цитогенетический анализ ДНК, выделенной из лимфоцитов пуповинной крови плода, с применением матричной сравнительной геномной гибридизации выявил частичную моносомию региона Хр22.2, размером 2,6 млн п.н.: arr[hg19] Xp22.2(11135472_13798048)×1. Наличие перестройки, а также ее de novo происхождение, подтверждено количественной ПЦР в режиме реального времени.
The first case of prenatal diagnosis of type I oro-facio-digital syndrome (OMIM 311200) in fetus with partial monosomy Xp22.2 is presented. The cause of type I oro-facio-digital syndrome is mutations in the OFDI gene, cases of disease due to chromosomal microdeletions affecting the OFDI gene are rare. Ultrasound examination of the fetus in the second trimester of pregnancy revealed congenital defect of brain development and multiple echographic markers of chromosomal and syndrome pathology. An umbilical cord blood was obtained in 21 weeks of gestation by cordocentesis. aCGH revealed partial monosomy Xp22.2 at 2.6 Mb in size: arr [hg19] Xp22.2(11135472_13798048) × 1. The presence of microdeletion, as well as its de novo origin, was confirmed by the quantitative real time PCR. The use of high-resolution molecular cytogenetic analysis significantly expands the possibilities of syndrome monogenic pathology diagnosis in the prenatal period.
Сomparative genomic hybridization in prenatal diagnosis
Authors: Гнетецкая В.А. , Тарасова Ю.А. , Кузнецова Е.С. , Ермакова М.А. , Ижевская В.Л. 
Published: November 2020

Молекулярное кариотипирование в нашем исследовании показало увеличение выявляемости хромосомных аберраций на 6,4% по сравнению c классическими цитогенетическими методами, однако для определения и/или уточнения природы микроскопических или субмикроскопических аномалий и для выявления сбалансированных перестроек необходимо их совместное применение.
Molecular karyotyping in our study showed a 6.4% increase in the detectability of chromosomal aberrations compared to classical cytogenetic methods, but their combined application is necessary to determine and/or clarify the nature of microscopic or submicroscopic anomalies and to identify balanced rearrangements.