bluuebunny/crossref_metadata_2025_split huggingface dataset:
Trabeculae microstructure parameters serve as effective predictors for marginal bone loss of dental implant in the mandible
Authors: Zhang Hengguo , Shan Jie , Zhang Ping , Chen Xin , Jiang Hongbing 
Published: October 2020

AbstractMarginal bone loss (MBL) is one of the leading causes of dental implant failure. This study aimed to investigate the feasibility of machine learning (ML) algorithms based on trabeculae microstructure parameters to predict the occurrence of severe MBL. Eighty-one patients (41 severe MBL cases and 40 normal controls) were involved in the current study. Four ML models, including support vector machine (SVM), artificial neural network (ANN), logistic regression (LR), and random forest (RF), were employed to predict severe MBL. The area under the receiver operating characteristic (ROC) curve (AUC), sensitivity, and specificity were used to evaluate the performance of these models. At the early stage of functional loading, severe MBL cases showed a significant increase of structure model index and trabecular pattern factor in peri-implant alveolar bone. The SVM model exhibited the best outcome in predicting MBL (AUC = 0.967, sensitivity = 91.67%, specificity = 100.00%), followed by ANN (AUC = 0.928, sensitivity = 91.67%, specificity = 93.33%), LR (AUC = 0.906, sensitivity = 91.67%, specificity = 93.33%), RF (AUC = 0.842, sensitivity = 75.00%, specificity = 86.67%). Together, ML algorithms based on the morphological variation of trabecular bone can be used to predict severe MBL.
Salinomycin induces autophagic cell death in salinomycin-sensitive melanoma cells through inhibition of autophagic flux
Authors: Liu Yajing , Hao Yinghua , Li Yuxia , Zheng Yadan , Dai Jiajing , Zhong Fubo , Wei Wei , Fang Zhengyu 
Published: October 2020

AbstractSeveral literature has shown that salinomycin (Sal) is able to kill various types of cancer cells through different signaling pathways. However, its effect on melanoma has seldom been reported. We examined the anti-cancer efficacy of Sal in melanoma cell lines, and found six of eight cell lines were sensitive to Sal. Given the fact that the roles of Sal are diverse in different cancer types, we were eager to figure out the mechanism involved in the current study. We noticed the most sensitive line, SK-Mel-19, showed a typical morphological change after Sal treatment. The autophagy inhibitor, 3-MA, could effectively suppress Sal-induced cell death. It could also facilitate the increase of autophagic markers and reduce the turnover of autophagosomes, which resulted in an aberrant autophagic flux. On the other hand, Sal could stimulate endoplasmic reticulum stress and cause an accumulation of dysfunctional mitochondria. We also discovered a potential correlation between LC3B mRNA level and its sensitivity to Sal in 43 clinical melanoma samples. Overall, our results indicated that Sal could have multiple effect on melanoma cells and induce autophagic cell death in certain kinds of cells, which provided a new insight into the chemotherapy for melanoma.
Endovascular treatment and cognitive outcome after anterior circulation ischemic stroke
Authors: Lattanzi Simona , Coccia Michela , Pulcini Alessandra , Cagnetti Claudia , Galli Federica Lucia , Villani Laura , Campa Serena , Dobran Mauro , Polonara Gabriele , Ceravolo Maria Gabriella , Silvestrini Mauro 
Published: October 2020

AbstractThe impact of reperfusion therapies on cognition has been poorly explored and little knowledge exists. We explored the influence of endovascular treatment (EVT) on cognitive outcome in patients with anterior circulation ischemic stroke. Patients presenting with ischemic stroke due to anterior large vessel occlusion who underwent intravenous thrombolysis (IVT) alone or EVT plus IVT were recruited. Cognitive abilities were evaluated at 6 months from stroke through a neuropsychological test battery. A total of 88 patients with a mean age of 66.3 ± 12.9 years were included, of which 38 treated with IVT alone and 50 with IVT plus EVT. Compared to patients treated with IVT alone, patients who received EVT plus IVT performed significantly better at the neuropsychological tests exploring executive functions, attention, abstract reasoning, visuospatial ability, visual and verbal and memory. At multivariable regression analysis, the EVT was independently associated with the 6-month cognitive performance after the adjustment for age, sex, admission National Institutes of Health Stroke Scale score, systolic blood pressure, glucose level, Alberta Stroke Program Early CT score, side of stroke, site of occlusion, and Back Depression Inventory score [Stroop Test Word Reading: adjβ = 13.99, 95% confidence interval (CI) 8.47–19.50, p < 0.001; Stroop Test Colour Naming: adjβ = 6.63, 95% CI 2.46–10.81, p = 0.002; Trail Making Test-A: adjβ = − 92.98, 95% CI − 153.76 to − 32.20, p = 0.003; Trail Making Test-B: adjβ = − 181.12, 95% CI − 266.09 to − 96.15; p < 0.001; Digit Span Test Forward: adjβ = 1.44, 95% CI 0.77–2.10, p < 0.001; Digit Span Test Backward: adjβ = 1.10, 95% CI 0.42–1.77, p = 0.002; Coloured Progressive Matrices: adjβ = 5.82, 95% CI 2.71–8.93, p < 0.001; Rey Complex Figure Test-Copy: adjβ = 6.02, 95% CI 2.74–9.30, p < 0.001; Rey Complex Figure Test-Immediate recall: adjβ = 6.00, 95% CI 2.34–9.66, p = 0.002; Rey Complex Figure Test-Delayed recall: adjβ = 5.73, 95% CI 1.95–9.51, p = 0.003; Rey Auditory Verbal Learning Test-Immediate recall: adjβ = 12.60, 95% CI 6.69–18.52, p < 0.001; Rey Auditory Verbal Learning Test-Delayed recall: adjβ = 1.85, 95% CI 0.24–3.45, p = 0.025]. Patients treated with EVT plus IVT had better cognitive performance than patients treated with IVT alone at 6 months from anterior circulation ischemic stroke.
Predictors of new-onset heart failure and overall survival in metastatic breast cancer patients treated with liposomal doxorubicin
Authors: Szmit Sebastian , Grela-Wojewoda Aleksandra , Talerczyk Małgorzata , Kufel-Grabowska Joanna , Streb Joanna , Smok-Kalwat Jolanta , Iżycki Dariusz , Chmielowska Ewa , Wilk Michał , Sosnowska-Pasiarska Barbara 
Published: October 2020

AbstractCardiovascular diseases (CVDs) are the major cause of morbidity/mortality among breast cancer (BC) patients. Observation of the daily practice in eight experienced Polish oncology centers was conducted to find all possible predictors of new cases of heart failure (HF) and overall survival (OS) of metastatic BC patients treated with liposomal doxorubicin, taking into account the impact of pre-existing CVDs. HF was the cause of premature discontinuation of liposomal doxorubicin therapy in 13 (3.2%) of 402 patients. The probability of developing HF was higher in women with pre-existing CVDs (HR 4.61; 95%CI 1.38–15.38). Independent of CVDs history, a lower risk of HF was observed in those treated with a cumulative dose of liposomal doxorubicin > 300 mg/m2 (HR 0.14; 95% CI 0.04–0.54) and taxane-naive (HR 0.26; 95% CI 0.07–0.96). Multivariate analysis including the presence of pre-existing CVDs and occurrence of new HF, revealed a liposomal doxorubicin in cumulative doses of > 300 mg/m2 as a beneficial predictor for OS (HR 0.61; 95% CI 0.47–0.78) independently of subsequent chemotherapy (HR 0.72; 95% CI 0.57–0.92) or endocrine therapy (HR 0.65; 95% CI 0.49–0.87). Higher doses of liposomal doxorubicin can decrease mortality in metastatic BC without increasing the risk of HF. The clinical benefit is achieved regardless of pre-existing CVDs and subsequent anticancer therapy.
Persistent abnormal muscle response after microvascular decompression for hemifacial spasm
Authors: Xu Li , Xu Wu , Wang Jing , Chong Yulong , Liang Weibang , Jiang Chengrong 
Published: October 2020

AbstractTo explore the causes of persistent abnormal muscle response (AMR) after microvascular decompression (MVD) for hemifacial spasm (HFS) and the clinical outcomes of these patients. MVDs performed in Nanjing Drum Tower Hospital in 2017 were retrospectively studied, and 326 patients with HFS were classified into two groups based on whether AMR disappeared or persisted following MVD. The clinical features, treatment efficacy and postoperative complications were compared between the two groups. 305 patients with disappeared AMR after decompression were classified as Group A. In Group B, the 21 patients exhibited persistent AMR after successful MVD. The preoperative duration of symptoms in Group B was significantly longer than that in Group A (P < 0.001), and no significant difference was identified between the two groups in terms of gender, side, age and offending vessels (P > 0.05). The immediate postoperative cure rate of Group A (88.9%)was significantly higher than that in Group B (28.6%,P < 0.001), furthermore, the two groups were not different in the long-term outcome and the incidence of surgical complications (P > 0.05). The long preoperative duration of HFS patients may account for persistent AMR after successful decompression, and it is more likely for these patients to get delayed cured, the long-term outcomes showed no difference compared to those in patients with disappeared AMR after MVD.
Masking terminal neo-epitopes of linear peptides through glycosylation favours immune responses towards core epitopes producing parental protein bound antibodies
Authors: Pon Robert , Marcil Anne , Chen Wangxue , Gadoury Christine , Williams Dean , Chan Kenneth , Zhou Hongyan , Ponce Amalia , Paquet Eric , Gurnani Komal , Chattopadhyay Anindita , Zou Wei 
Published: October 2020

AbstractGlycosylation of hydrophobic peptides at one terminus effectively increases their water-solubility, and conjugation through the opposing end to a carrier protein, renders them more immunogenic. Moreover, the glycosylation minimizes antibody responses to potentially deleterious, non-productive terminal neo-epitope regions of the peptides, and consequently shifts peptide immunogenicity towards the core amino acid residues. As proof of concept, glycopeptide-protein conjugates related to influenza hemagglutinin (HA), neuraminidase (NA), and the dimerization loop region of human epidermal growth factor receptor 2 (Her2), demonstrated a favorable production of core peptide specific antibodies as determined by ELISA studies. Furthermore, glycosylated Her2 peptide conjugate antisera were also shown to recognize full length Her2 protein by ELISA and at the cell surface through flow cytometry analysis. In contrast, unmasked peptide conjugates generated significant antibody populations that were specific to the terminal neo-epitope of the peptide immunogen that are notably absent in parental proteins. Antibodies generated in this manner to peptides in the dimerization loop of Her2 are also functional as demonstrated by the growth inhibition of Her2 expressing SKBR3 carcinoma cells. This method provides a technique to tailor-make epitope-specific antibodies that may facilitate vaccine, therapeutic and diagnostic antibody development.
Total saponins from Rubus parvifolius L. inhibits cell proliferation, migration and invasion of malignant melanoma in vitro and in vivo
Authors: Cao Jinfeng , Zhao Xue , Ma Yan , Yang Jian , Li Fuqiang 
Published: October 2020

Abstract
Background: Total saponins from Rubus parvifolius L. (TSRP) are the main bioactive fractions responsible for the anti-tumor activities. The work was aimed to evaluate the anti-tumor effect of TSRP in malignant melanoma (MM) in vitro and in vivo.
Methods and results: Anti-melanoma cell proliferation, invasion and migration effect of TSRP were detected in human MM A375 cells under the indicated time and dosages. In vivo anti-tumor effect of TSRP was measured in A375 xenograft immunodeficient nude mice. Sixty A375 xenografts were randomly divided into five groups: Vehicle, cyclophosphamide (CTX, 20 mg/kg), TSRP (25 mg/kg), TSRP (50 mg/kg) and TSRP (100 mg/kg) groups for 14 days’ treatment. In addition, the melanoma metastasis in lung in vivo of TSRP was detected in A375 tail vein injection mice, and the histopathalogical analysis of the lung metastasis was detected by Hematoxylin–Eosin (H&E) staining. TSRP significantly inhibited the cell proliferation, invasion and migration of A375 in vitro at the indicated time and dosages. TSRP treatment effectively blocked the tumor growth in immunodeficient nude mice. In addition, TSRP also significantly inhibited the lung metastasis of melanoma.
Conclusion: The present study indicated that the TSRP has a remarkable anti-MM effect, which mainly through the inhibition of the cell invasion, migration and tumor metastasis.
Capturing a Moment in Time: A Survey of School-Based Speech-Language Pathologists' Experiences in the Immediate Aftermath of the COVID-19 Public Health Emergency
Authors: Sylvan Lesley , Goldstein Erica , Crandall Megan 
Published: October 2020

Purpose
Data collected by the American Speech-Language-Hearing Association in the spring of 2020 showed that most speech-language pathologists (SLPs) experienced a moderate to major impact on their work practices as a result of the COVID-19 public health emergency. This study focused on gaining a deeper understanding of the experiences of school-based SLPs at this unique moment in time.

Method
This study involved a survey of 280 school-based SLPs working across the United States in May 2020. The results of the survey were analyzed both qualitatively and quantitatively to identify key themes and trends related to participants' confidence levels, workload changes, and personal challenges.

Results
Results indicated that SLPs faced variation in the guidance, communication, and resources they were provided by employers and variations regarding the timelines and expectations for the pivot from traditional face-to-face school services to remote services. Few SLPs had previous experience with teletherapy, and many reported low levels of self-confidence and large increases in their workload. Additionally, SLPs faced high levels of stress in their personal lives.

Conclusions
Despite the challenges faced, participants largely reported they were able to adapt with the support of the SLP community. Participants reported concerns regarding the relative success of their students under new service delivery models, especially for students from disadvantaged groups, and worries regarding future legal challenges. This study identifies key issues to consider with respect to speech-language services in schools as the COVID-19 public health emergency continues to unfold.
Differenzialdiagnose ausgedehnter ossärer Destruktionen
– ein Fallbericht
Authors: Engel Kristin , Kayser Marten , Unger Leonore 
Published: October 2020

ZusammenfassungDie Gicht ist die häufigste metabolische Arthropathie. Obwohl
Ätiologie und Pathogenese des Krankheitsbildes gut untersucht sind,
bereitet die Diagnosesicherung mittels des Nachweises von
Natriumuratkristallen aus einem Gelenkpunktat oder anderen betroffenen
Geweben mitunter große Schwierigkeiten. Wir berichten von einem
außergewöhnlich schweren Fall einer generalisierten
Gichtarthropathie mit atypischer Manifestation und kompliziertem Verlauf.
Dabei sollen wichtige Differenzialdiagnosen ausgedehnter ossärer
Destruktionen beleuchtet werden.
Osteoid Osteoma of the Atlas in a Boy: Clinical and Imaging Features–A Case Report and Review of the Literature
Authors: Valluzzi Adelaide , Donatiello Salvatore , Gallo Graziana , Cellini Monica , Maiorana Antonino , Spina Vincenzo , Pavesi Giacomo 
Published: October 2020

AbstractOsteoid osteoma is a benign osteoblastic tumor, quite uncommon in the spine. We report a case of an osteoid osteoma involving the atlas in a 6-year-old boy, who presented with suboccipital pain and torticollis. Initial radiological findings were ambiguous as magnetic resonance imaging showed mainly edema of upper cervical soft tissues. The subsequent computed tomography depicted a lesion of left lamina of C1. As conservative treatment failed, the lesion was surgically resected and the patient became pain free. To our knowledge, this is the first case of osteoid osteoma involving the atlas associated with abnormal soft tissue reaction reported in literature.
Development of a Taxonomy for Medication-Related Patient Safety Events Related to Health Information Technology in Pediatrics
Authors: Wyatt Kirk D. , Benning Tyler J. , Morgenthaler Timothy I. , Arteaga Grace M. 
Published: October 2020

AbstractBackground Although electronic health records (EHRs) are designed to improve patient safety, they have been associated with serious patient harm. An agreed-upon and standard taxonomy for classifying health information technology (HIT) related patient safety events does not exist.Objectives We aimed to develop and evaluate a taxonomy for medication-related patient safety events associated with HIT and validate it using a set of events involving pediatric patients.Methods We performed a literature search to identify existing classifications for HIT-related safety events, which were assessed using real-world pediatric medication-related patient safety events extracted from two sources: patient safety event reporting system (ERS) reports and information technology help desk (HD) tickets. A team of clinical and patient safety experts used iterative tests of change and consensus building to converge on a single taxonomy. The final devised taxonomy was applied to pediatric medication-related events assess its characteristics, including interrater reliability and agreement.Results Literature review identified four existing classifications for HIT-related patient safety events, and one was iteratively adapted to converge on a singular taxonomy. Safety events relating to usability accounted for a greater proportion of ERS reports, compared with HD tickets (37 vs. 20%, p = 0.022). Conversely, events pertaining to incorrect configuration accounted for a greater proportion of HD tickets, compared with ERS reports (63 vs. 8%, p < 0.01). Interrater agreement (%) and reliability (kappa) were 87.8% and 0.688 for ERS reports and 73.6% and 0.556 for HD tickets, respectively.Discussion A standardized taxonomy for medication-related patient safety events related to HIT is presented. The taxonomy was validated using pediatric events. Further evaluation can assess whether the taxonomy is suitable for nonmedication-related events and those occurring in other patient populations.Conclusion Wider application of standardized taxonomies will allow for peer benchmarking and facilitate collaborative interinstitutional patient safety improvement efforts.
Rehabilitation of a Patient with Temporomandibular Joint Disorder and Misocclusion Requiring Full Mouth Reconstruction, Based on the Multidisciplinary Approach: A Clinical Case
Authors: Dzalaeva Fatima , Chikunov Sergey , Utyuzh Anatoly , Mikhailova Maria , Budunova Marzhanat 
Published: October 2020

AbstractThis case report involved the development of a rehabilitation system for patient with edentulism and an algorithm for diagnostic and therapeutic measures to be planned based on the patient’s individual anatomical and physiological features. The results of treatment and rehabilitation made it possible to achieve the optimal distribution of loads on the dentition. It was shown that it is necessary to analyze casts in an articulator to reproduce a three-dimensional model of mandibular movements taking into account the individual features of the patient to obtain a satisfactory assessment and treatment planning of patients with edentulism requiring full mouth reconstruction. This case confirms that the developed algorithm is anatomically and pathogenetically justified, as it considers all changes and interrelationships of structures of the dentition.
Providing Inpatient Mobilization with a Mobility Technician Constrains Cost in Primary Total Knee Arthroplasty
Authors: Novack Thomas A. , Mazzei Christopher J. , Patel Jay N. , Poletick Eileen B. , D'Achille Roberta , Wittig James C. 
Published: October 2020

AbstractSince the 2016 implementation of the comprehensive care for joint replacement (CJR) bundled payment model, our institutions have sought to decrease inpatient physical therapy (PT) costs by piloting a mobility technician program (MTP), where mobility technicians (MTs) ambulate postoperative total knee arthroplasty (TKA) patients under the supervision of nursing staff members. MTs are certified medical assistants given specialized gate and ambulation training by the PT department. The aim of this study was to examine the economic and clinical impact of MTs on the primary TKA postoperative pathway. We performed a retrospective review of TKA patients who underwent surgery at our institution between April 2018 and March 2019 and who were postoperatively ambulated by MTs. The control group included patients who had surgery during the same months of the prior year, preceding introduction of MTs to the floor. Inclusion criteria included: unilateral primary TKA for arthritic conditions and conversion to unilateral primary TKA from a previous knee surgery. Minitab Software (State College, PA) was used to perform the statistical analysis. There were 658 patients enrolled in the study group and 1,400 in the control group. The two groups shared similar demographics and an average age of 68 (p = 0.177). The median length of stay (LOS) was 2 days in both groups (p = 0.133) with 90.5% of patients in the study group discharged to home versus 81.5% of patients in the control group (p < 0.001). The ability of MTs to increase patient discharge to home without negatively impacting LOS suggest MTs are valuable both clinically to patients, and economically to the institution. Cost analysis highlighted the substantial cost savings that MTs may create in a bundled payment system. With the well-documented benefits of early ambulation following TKA, we demonstrate how MTs can be an asset to optimizing the care pathway of TKA patients.
Mechanisms Underlying Abnormal Expression of lncRNA H19 in Neonatal Hypoxic–Ischemic Encephalopathy
Authors: Zhu Hongwei , Wang Lei , Chen Juanjuan , Shen Huaiyun , Chen Zhenxiang 
Published: October 2020

Objective Hypoxic–ischemic (HI)-related brain injury, especially HI encephalopathy (HIE) is a leading cause of morbidity and disability in newborns. Long noncoding RNAs (lncRNAs) are implicated in the progress of HI brain damage. However, the mechanisms underlying the regulatory effects of lncRNA H19 on autophagy in HIE remain unknown. This study was designed to identify the potential mechanisms involving lncRNA H19 in HIE.
Study Design We selected three HIE newborns and three healthy newborns for neonatal behavioral neurological assessment and screened the differentially expressed lncRNAs by microarray analysis and detected H19 expression in serum. After that, neonatal HIE rats were established and injected with H19 overexpression lentivirus vector or autophagy activator Rapa. The structure and apoptotic levels of brain tissue were observed, and righting reflex and geotaxis reflex were utilized to evaluate the short-term neurological function of HIE rats. The Morris water maze was performed to measure the long-term neurological functions of HIE rats. The binding relationships among H19/miR-19b/protein kinase B3 (Akt3) were verified. Levels of Akt3- and autophagy-related proteins were measured.
Results H19 was upregulated in HIE newborns and rat models. The areas of cerebral infarction and apoptosis in neonatal HIE rats were increased, and the nerve functions were compromised. The overexpression of H19 alleviated nerve damage of neonatal HIE rats, and reduced autophagy of brain tissue. H19 upregulated Akt3 as a miR-29b sponge. The protective effects of overexpression of H19 on brain tissue and nerve functions of neonatal HIE rats were partially reversed by autophagy activator.
Conclusion H19 improved the brain tissue and alleviated nerve damage of neonatal HIE rats by upregulating the Akt3/mTOR pathway as a miR-29b sponge.
Key Points