bluuebunny/crossref_metadata_2025_split huggingface dataset:
Integrative proteomic and functional analyses provide novel insights into the action of the repurposed drug candidate nitroxoline in AsPC-1 cells
Authors: Veschi Serena , Ronci Maurizio , Lanuti Paola , De Lellis Laura , Florio Rosalba , Bologna Giuseppina , Scotti Luca , Carletti Erminia , Brugnoli Federica , Di Bella Maria Cristina , Bertagnolo Valeria , Marchisio Marco , Cama Alessandro 
Published: February 2020

AbstractWe recently identified nitroxoline as a repurposed drug candidate in pancreatic cancer (PC) showing a dose-dependent antiproliferative activity in different PC cell lines. This antibiotic is effective in several in vitro and animal cancer models. To date, the mechanisms of nitroxoline anticancer action are largely unknown. Using shotgun proteomics we identified 363 proteins affected by nitroxoline treatment in AsPC-1 pancreatic cancer cells, including 81 consistently deregulated at both 24- and 48-hour treatment. These proteins previously unknown to be affected by nitroxoline were mostly downregulated and interconnected in a single highly-enriched network of protein-protein interactions. Integrative proteomic and functional analyses revealed nitroxoline-induced downregulation of Na/K-ATPase pump and β-catenin, which associated with drastic impairment in cell growth, migration, invasion, increased ROS production and induction of DNA damage response. Remarkably, nitroxoline induced a previously unknown deregulation of molecules with a critical role in cell bioenergetics, which resulted in mitochondrial depolarization. Our study also suggests that deregulation of cytosolic iron homeostasis and of co-translational targeting to membrane contribute to nitroxoline anticancer action. This study broadens our understanding of the mechanisms of nitroxoline action, showing that the drug modulates multiple proteins crucial in cancer biology and previously unknown to be affected by nitroxoline.
Does sediment composition sort kinorhynch communities? An ecomorphological approach through geometric morphometrics
Authors: Cepeda Diego , Trigo Dolores , Pardos Fernando , Sánchez Nuria 
Published: February 2020

AbstractEcomorphology studies the relationship between organisms’ morphology and environment features. To better understand whether the shape of the body and the appendages involved in the movement is correlated to sediment composition in meiofaunal organisms, we study the evolved morphological adaptations to environment in selected taxa of the phylum Kinorhyncha: the allomalorhagid families Dracoderidae and Pycnophyidae, and the cyclorhagid genus Echinoderes. The selected taxa include the most diverse groups of Kinorhyncha worldwide, representing the 75.5% of the total phylum diversity. Widened, plump bodies and lateral terminal spines may be adaptive for species living in coarse, more heterogeneous sediments, as they could maintain a more powerful musculature to actively displace the sediment grains applying a greater force. Conversely, slender, vermiform bodies and lateral terminal spines would represent an adaptation of species inhabiting fine, more homogeneous sediments where there would not be much need to exert a high force to displace the sediment particles, and a more vermiform shape would even favour the burrowing of the animal through the smaller interstices. The studied kinorhynch taxa would also be adapted to the higher velocity of the sea-water and the intense erosion and transportation of heterogeneous sediments by possessing more robust bodies, avoiding getting laid off substratum under these conditions. These findings provide evolutionary evidence that body shape in the studied kinorhynch groups is adapted to environment.
Inhibition of cyclooxygenase-1 by nonsteroidal anti-inflammatory drugs demethylates MeR2 enhancer and promotes Mbnl1 transcription in myogenic cells
Authors: Huang Kun , Masuda Akio , Chen Guiying , Bushra Samira , Kamon Masayoshi , Araki Toshiyuki , Kinoshita Masanobu , Ohkawara Bisei , Ito Mikako , Ohno Kinji 
Published: February 2020

AbstractMuscleblind-like 1 (MBNL1) is a ubiquitously expressed RNA-binding protein, which is highly expressed in skeletal muscle. Abnormally expanded CUG-repeats in the DMPK gene cause myotonic dystrophy type 1 (DM1) by sequestration of MBNL1 to nuclear RNA foci and by upregulation of another RNA-binding protein, CUG-binding protein 1 (CUGBP1). We previously reported that a nonsteroidal anti-inflammatory drug (NSAID), phenylbutazone, upregulates MBNL1 expression in DM1 mouse model by demethylation of MeR2, an enhancer element in Mbnl1 intron 1. NSAIDs inhibit cyclooxygenase (COX), which is comprised of COX-1 and COX-2 isoforms. In this study, we screened 29 NSAIDs in C2C12 myoblasts, and found that 13 NSAIDs enhanced Mbnl1 expression, where COX-1-selective NSAIDs upregulated Mbnl1 more than COX-2-selective NSAIDs. Consistently, knockdown of COX-1, but not of COX-2, upregulated MBNL1 expression in C2C12 myoblasts and myotubes, as well as in myotubes differentiated from DM1 patient-derived induced pluripotent stem cells (iPSCs). Luciferase assay showed that COX-1-knockdown augmented the MeR2 enhancer activity. Furthermore, bisulfite sequencing analysis demonstrated that COX-1-knockdown suppressed methylation of MeR2. These results suggest that COX-1 inhibition upregulates Mbnl1 transcription through demethylation of the MeR2 enhancer. Taken together, our study provides new insights into the transcriptional regulation of Mbnl1 by the COX-1-mediated pathway.
Sweet potato viromes in eight different geographical regions in Korea and two different cultivars
Authors: Jo Yeonhwa , Kim Sang-Min , Choi Hoseong , Yang Jung Wook , Lee Bong Choon , Cho Won Kyong 
Published: February 2020

AbstractThe sweet potato in the family Convolvulaceae is a dicotyledonous perennial plant. Here, we conducted a comprehensive sweet potato virome study using 10 different libraries from eight regions in Korea and two different sweet potato cultivars by RNA-Sequencing. Comprehensive bioinformatics analyses revealed 10 different virus species infecting sweet potato. Moreover, we identified two novel viruses infecting sweet potato referred to as Sweet potato virus E (SPVE) in the genus Potyvirus and Sweet potato virus F (SPVF) in the genus Carlavirus. Of the identified viruses, Sweet potato feathery mottle virus (SPFMV) was the dominant virus followed by Sweet potato virus C (SPVC) and SPVE in Korea. We obtained a total of 30 viral genomes for eight viruses. Our phylogenetic analyses showed many potyvirus isolates are highly correlated with geographical regions. However, two isolates of SPFMV and a single isolate of Sweet potato virus G (SPVG) were genetically distant from other known isolates. The mutation rate was the highest in SPFMV followed by SPVC and SPVG. Two different sweet potato cultivars, Beni Haruka and Hogammi, were infected by seven and five viruses, respectively. Taken together, we provide a complete list of viruses infecting sweet potato in Korea and diagnostic methods.
Effects of Compound Sand Barrier for Habitat Restoration on Sediment Grain-size Distribution in Ulan Buh Desert
Authors: Pan Xia , Wang Zhenyi , Gao Yong 
Published: February 2020

AbstractWind erosion is a huge challenge for ecologists to stabilize sand dunes and to change them into stable productive ecosystems. In order to better understand its role in the process of ecological restoration, the sediment grain-size characteristics of compound sand barrier were evaluated through field experimental observation. The results indicated that the compound sand barrier was mainly composed of extremely fine sand and fine sand, and the fine sand and extremely fine sand in the inner side were higher than the east and west sides of the compound sand barrier. Due to the blocking effect of compound sand barrier, the Sorting Coefficient became better, the Skewness belonged to the positive deviation and the Kurtosis presented leptokurtosis distribution. Moreover, while the cumulative frequency distribution curve in the inner side became steeper, the slope increased and reached the top of the curve ahead of time. The effect of wind environment and vegetation coverage on the surface sediments showed that the average annual wind velocity and vegetation coverage was negatively correlated with the average grain-size, but positively correlated with the Sorting Coefficient. There was a significant correlation among the annual wind speed, vegetation coverage, average grain-size and Sorting Coefficient, which indicated that vegetation coverage and wind environment was the key factor leading to the difference of surface sediments in this area. Collectively, the establishment of compound sand barrier is one of the most effective methods of sand-fixing with engineering measure in the arid desert regions. Therefore, given the complexities of agricultural systems, stubble retention and black film covered during harvesting and incorporation of the stubble into soil in the next spring appears to be the best choice in the dry northern China where farmlands suffer serious wind erosion.
Using genomics to understand the origin and dispersion of multidrug and extensively drug resistant tuberculosis in Portugal
Authors: Perdigão João , Gomes Pedro , Miranda Anabela , Maltez Fernando , Machado Diana , Silva Carla , Phelan Jody E. , Brum Laura , Campino Susana , Couto Isabel , Viveiros Miguel , Clark Taane G. , Portugal Isabel 
Published: February 2020

AbstractPortugal is a low incidence country for tuberculosis (TB) disease. Now figuring among TB low incidence countries, it has since the 1990s reported multidrug resistant and extensively drug resistant (XDR) TB cases, driven predominantly by two strain-types: Lisboa3 and Q1. This study describes the largest characterization of the evolutionary trajectory of M/XDR-TB strains in Portugal, spanning a time-period of two decades. By combining whole-genome sequencing and phenotypic susceptibility data for 207 isolates, we report the geospatial patterns of drug resistant TB, particularly the dispersion of Lisboa3 and Q1 clades, which underly 64.2% and 94.0% of all MDR-TB and XDR-TB isolates, respectively. Genomic-based similarity and a phylogenetic analysis revealed multiple clusters (n = 16) reflecting ongoing and uncontrolled recent transmission of M/XDR-TB, predominantly associated with the Lisboa3 and Q1 clades. These clades are now thought to be evolving in a polycentric mode across multiple geographical districts. The inferred evolutionary history is compatible with MDR- and XDR-TB originating in Portugal in the 70’s and 80’s, respectively, but with subsequent multiple emergence events of MDR and XDR-TB particularly involving the Lisboa3 clade. A SNP barcode was defined for Lisboa3 and Q1 and comparison with a phylogeny of global strain-types (n = 28 385) revealed the presence of Lisboa3 and Q1 strains in Europe, South America and Africa. In summary, Portugal displays an unusual and unique epidemiological setting shaped by >40 years of uncontrolled circulation of two main phylogenetic clades, leading to a sympatric evolutionary trajectory towards XDR-TB with the potential for global reach.
ACTsmart – development and feasibility of digital Acceptance and Commitment Therapy for adults with chronic pain
Authors: Gentili Charlotte , Zetterqvist Vendela , Rickardsson Jenny , Holmström Linda , Simons Laura E. , Wicksell Rikard K. 
Published: February 2020

AbstractAccessibility of evidence-based behavioral health interventions is one of the main challenges in health care and effective treatment approaches are not always available for patients that would benefit from them. Digitization has dramatically changed the health care landscape. Although mHealth has shown promise in addressing issues of accessibility and reach, there is vast room for improvements. The integration of technical innovations and theory driven development is a key concern. Digital solutions developed by industry alone often lack a clear theoretical framework and the solutions are not properly evaluated to meet the standards of scientifically proven efficacy. On the other hand, mHealth interventions developed in academia may be theory driven but lack user friendliness and are commonly technically outdated by the time they are implemented in regular care, if they ever are. In an ongoing project aimed at scientific innovation, the mHealth Agile Development and Evaluation Lifecycle was used to combine strengths from both industry and academia in the development of ACTsmart – a smartphone-based Acceptance and Commitment Therapy treatment for adult chronic pain patients. The present study describes the early development of ACTsmart, in the process of moving the product from alpha testing to a clinical trial ready solution.
Cassiosomes are stinging-cell structures in the mucus of the upside-down jellyfish Cassiopea xamachana
Authors: Ames Cheryl L. , Klompen Anna M. L. , Badhiwala Krishna , Muffett Kade , Reft Abigail J. , Kumar Mehr , Janssen Jennie D. , Schultzhaus Janna N. , Field Lauren D. , Muroski Megan E. , Bezio Nick , Robinson Jacob T. , Leary Dagmar H. , Cartwright Paulyn , Collins Allen G. , Vora Gary J. 
Published: February 2020

AbstractSnorkelers in mangrove forest waters inhabited by the upside-down jellyfish Cassiopea xamachana report discomfort due to a sensation known as stinging water, the cause of which is unknown. Using a combination of histology, microscopy, microfluidics, videography, molecular biology, and mass spectrometry-based proteomics, we describe C. xamachana stinging-cell structures that we term cassiosomes. These structures are released within C. xamachana mucus and are capable of killing prey. Cassiosomes consist of an outer epithelial layer mainly composed of nematocytes surrounding a core filled by endosymbiotic dinoflagellates hosted within amoebocytes and presumptive mesoglea. Furthermore, we report cassiosome structures in four additional jellyfish species in the same taxonomic group as C. xamachana (Class Scyphozoa; Order Rhizostomeae), categorized as either motile (ciliated) or nonmotile types. This inaugural study provides a qualitative assessment of the stinging contents of C. xamachana mucus and implicates mucus containing cassiosomes and free intact nematocytes as the cause of stinging water.
Deletion of a conserved Gata2 enhancer impairs haemogenic endothelium programming and adult Zebrafish haematopoiesis
Authors: Dobrzycki Tomasz , Mahony Christopher B. , Krecsmarik Monika , Koyunlar Cansu , Rispoli Rossella , Peulen-Zink Joke , Gussinklo Kirsten , Fedlaoui Bakhta , de Pater Emma , Patient Roger , Monteiro Rui 
Published: February 2020

AbstractGata2 is a key transcription factor required to generate Haematopoietic Stem and Progenitor Cells (HSPCs) from haemogenic endothelium (HE); misexpression of Gata2 leads to haematopoietic disorders. Here we deleted a conserved enhancer (i4 enhancer) driving pan-endothelial expression of the zebrafish gata2a and showed that Gata2a is required for HE programming by regulating expression of runx1 and of the second Gata2 orthologue, gata2b. By 5 days, homozygous gata2aΔi4/Δi4 larvae showed normal numbers of HSPCs, a recovery mediated by Notch signalling driving gata2b and runx1 expression in HE. However, gata2aΔi4/Δi4 adults showed oedema, susceptibility to infections and marrow hypo-cellularity, consistent with bone marrow failure found in GATA2 deficiency syndromes. Thus, gata2a expression driven by the i4 enhancer is required for correct HE programming in embryos and maintenance of steady-state haematopoietic stem cell output in the adult. These enhancer mutants will be useful in exploring further the pathophysiology of GATA2-related deficiencies in vivo.
Carbohydrate antigen-125, calcium, and hemoglobin as predictive clinical indicator for ocular metastasis in male liver cancer patients
Authors: Xu Qian-Hui , Zhu Pei-Wen , Li Biao , Shi Wen-Qing , Lin Qi , Min You-Lan , Ge Qian-Min , Yuan Qing , Shao Yi 
Published: February 2020

Abstract
Background Primary liver cancer (PLC) is a common type of cancer among men worldwide. Little is known regarding the relationship of liver cancer with ocular metastasis (OM). Drinking has been also reported to be related not only to the occurrence of liver cancer but also to the causes of some ocular lesions.
Purpose A diagnostic standard for the levels of serum biomarkers associated with OM derived from liver cancer in men is urgently needed.
Material and methods We examined the association between OM in liver cancer and its serum biomarkers. A total of 1254 male patients with liver cancer were recruited in this retrospective study between July 2002 and December 2012. We assessed the relationship between drinking preference and OM in male patients with liver cancer, and aimed to identify an independent prognostic factor or establish a quantitative indicator for OM.
Results By assessing the potential indicators, carbohydrate antigen-125 (CA-125), calcium, and hemoglobin (Hb) were found to be most valuable in the diagnosis of OM in male patients with liver cancer.
Conclusion CA-125, calcium, and Hb are independent risk factors of OM in patients with liver cancer who consume alcohol.
Einfluss der Imitation von Bewegungen im Vergleich zur verbalen oder autodidaktischen Übungsanleitung
Authors: Keller Aileen 
Published: February 2020

ZusammenfassungFür genaue Bewegungsausführungen und bessere Behandlungsergebnisse ist bei der Kommunikation mit Patienten eine präzise Übungsanleitung nötig. Bisher existieren
die imitative, verbale und autodidaktische Übungsanleitung.Die vorliegende empirische Studie untersuchte diese drei Formen der Übungsanleitung am Beispiel des Deep Squat aus dem „Functional Movement Screen“, um die
Anleitung mit dem höchsten Nutzen für Patienten herauszufinden und Empfehlungen für die physiotherapeutische Behandlung zu geben.Die in die Studie eingeschlossenen 51 gesunden Probanden wurden gleichermaßen auf eine Interventionsgruppe (imitative Übungsanleitung in Form eines Videos) und
zwei Vergleichsgruppen (verbale bzw. autodidaktische Anleitung) aufgeteilt.Die Übungsanleitung via Imitation erzielte die höchste Präzision der Bewegungsausführung.
Testsequenzen für den Praktiker
Authors: Kubat Heike , Schulze Elke 
Published: February 2020

ZusammenfassungWas macht eine „gute Sensomotorik“ in der kraniozervikalen Region aus? Bei Patienten mit kraniozervikalen Symptomen (Nacken-, Kopf- und Gesichtsschmerzen,
Schwindel, Gleichgewichtsstörungen oder Ohrsensationen) ist häufig die sensomotorische Leistungsfähigkeit gestört.Dieser 3. Teil der Artikelreihe beschreibt spezifische sensomotorische Tests zur Überprüfung der notwendigen HWS-Funktionen wie den Joint-Position-Error- und
modifizierten Fly-Test sowie sequenzielle Kopf- und Augenbewegungen.
Diagnostik der pAVK – arterielle Verschlussdruckmessung
Authors: Mühlberger Dominic , Ahrendt Johann W. , Mumme Achim , Hummel Thomas 
Published: February 2020

ZusammenfassungDie periphere arterielle Verschlusskrankheit (pAVK) ist ein häufiges Krankheitsbild mit einer zunehmenden Prävalenz im Alter. Ursache dafür ist in über 90 % der Fälle eine Arteriosklerose. Patienten mit einem erniedrigten Knöchel-Arm-Index (ankle-brachial-index, ABI) weisen, sowohl asymptomatisch als auch symptomatisch, eine erhöhte kardiovaskuläre Morbidität und Mortalität auf. Daher ist die Dopplerverschlussdruckmessung mit Erhebung des ABI von zentraler Bedeutung. Ein ABI von unter 0,9 gilt als Grenzwert für eine pAVK. Aber auch ein erhöhter ABI von über 1,3 ist, als Zeichen einer Mediasklerose, häufig bei Patienten mit einem Diabetes mellitus oder chronischen Nierenerkrankungen vorzufinden und ebenfalls als pathologisch zu werten.
New Technology of Thermoplastic Coating for Osmotic Pump Tablets: Study on in vivo Drug Release
Authors: Yuan Chun Ping , Hou Hui Min , Cheng Zhi Hong , Ge Qing Hua , Song Ding Zhong , Xi Jian Qiang 
Published: February 2020

Abstract
Aim The in vivo pharmacokinetics of thermoplastic-coated tablets prepared by a new technology of thermoplastic coating in Beagle dogs were studied, and the correlation between in vitro release and in vivo absorption was analyzed.
Methods The in vitro release profiles of metformin hydrochloride thermoplastic-coated tablets and nifedipine thermoplastic-coated tablets were investigated. The single-dose pharmacokinetic study of these tablets in Beagle dogs was performed, and the obtained results were separately compared with the data of conventional osmotic pump tablets reported in the literature.
Results Metformin hydrochloride thermoplastic-coated tablets and nifedipine thermoplastic-coated tablets displayed controlled drug-release characteristics and had a good in vivo–in vitro correlation in Beagle dogs, respectively. The literature-compared results further demonstrated that both thermoplastic-coated tablets had release characteristics of osmotic pump tablets in vivo.
Conclusion The thermoplastic-coated tablets could control drug release in vivo and it was further confirmed that the new thermoplastic coating technology could replace the spray coating of osmotic pump controlled-release tablets. This study provides a theoretical basis and practical support for the industrialization and clinical application of the new thermoplastic coating technology.
Pseudohypoparathyroidism with Ectopic Calcification and 22q11 Deletion Syndrome: A Rare Case
Authors: Diniz Bruna Lixinski , Glaeser Andressa Barreto , Deconte Desirée , Guaraná Bruna Baierle , Rosa Rafael Fabiano Machado , Zen Paulo Ricardo Gazzola 
Published: February 2020

AbstractEctopic calcification in soft tissue is associated with several disorders including pseudohypoparathyroidism (PHP), which is characterized by resistance or nonresponse to parathyroid hormone (PTH) function. Association between PHP and 22q11DS, also known as DiGeorge syndrome, is rare, especially in children. We describe a newborn girl diagnosed with 22q11DS, presenting ectopic calcifications in soft tissue and suspicion of PHP. PTH function showed values close to the upper limit of the reference value. Radiology showed bone callus in the right wrist. PHP can be a new clinical finding associated with 22q11DS. Parathyroid function investigation in individuals with 22q11DS, presenting bone dysmorphisms and/or calcium metabolism alterations, should be considered.
Übersetzung von Gerichtsdokumenten
Authors: Nauen Milana 
Published: February 2020

AbstractThe article examines the translation of court documents (indictments, penalty and arrest warrants, court decisions and court orders) and the challenges they pose. The activity of court translators is regulated by law. This unique position entrusts them with a special responsibility. Even if the actual addressees of the translation are unlikely to fully understand the text, the translation must be accurate and complete. This requires a thorough knowledge of both legal systems and an understanding of both legal languages. The present article discusses parts of a court document from a translator’s point of view, analysing the difficulties of translating court names, titles of laws, classification units and standardised formulations. The article contains many examples from the language pair German-Russian but is also comprehensible and of potential interest for translators from and into other languages.