bluuebunny/crossref_metadata_2025_split huggingface dataset:
Seafloor incubation experiment with deep-sea hydrothermal vent fluid reveals effect of pressure and lag time on autotrophic microbial communities
Authors: Fortunato Caroline S. , Butterfield David A. , Larson Benjamin , Lawrence-Slavas Noah , Algar Christopher K. , Allen Lisa Zeigler , Holden James F. , Proskurowski Giora , Reddington Emily , Stewart Lucy C. , Topçuoğlu Begüm D. , Vallino Joseph J. , Huber Julie A. 
Published: November 2020

AbstractDepressurization and sample processing delays may impact the outcome of shipboard microbial incubations of samples collected from the deep sea. To address this knowledge gap, we developed an ROV-powered incubator instrument to carry out and compare results fromin situand shipboard RNA Stable Isotope Probing (RNA-SIP) experiments to identify the key chemolithoautotrophic microbes and metabolisms in diffuse, low-temperature venting fluids from Axial Seamount. All the incubations showed microbial uptake of labelled bicarbonate primarily by thermophilic autotrophic Epsilonbacteraeota that oxidized hydrogen coupled with nitrate reduction. However, thein situseafloor incubations showed higher abundances of transcripts annotated for aerobic processes suggesting that oxygen was lost from the hydrothermal fluid samples prior to shipboard analysis. Furthermore, transcripts for thermal stress proteins such as heat shock chaperones and proteases were significantly more abundant in the shipboard incubations suggesting that hydrostatic pressure ameliorated thermal stress in the metabolically active microbes in the seafloor incubations. Together, results indicate that while the autotrophic microbial communities in the shipboard and seafloor experiments behaved similarly, there were distinct differences that provide new insight into the activities of natural microbial assemblages under near-native conditions in the ocean.
Zona Incerta subpopulations differentially encode and modulate anxiety
Authors: Li Zhuoliang , Rizzi Giorgio , Tan Kelly R. 
Published: November 2020

AbstractDespite recent clinical observations linking the Zona Incerta (ZI) to anxiety, little is known about whether and how the ZI processes anxiety. Here, we subject mice to anxious experiences and observe an increase in ZI cfos-labelled neurons and single-cell calcium activity as well as an efficient effect of ZI infusion of diazepam a classical anxiolytic drug. We further identify that somatostatin-(SOM), calretinin-(CR), and vesicular glutamate transporter-2 (Vglut2)-expressing cells display unique electrophysiological profiles; yet they similarly respond to anxiety-provoking stimuli and to diazepam. Interestingly, optogenetic manipulations reveals that each of these ZI neuronal population triggers specific anxiety-related behavioral phenotypes. Activation of SOM-expressing neurons induced anxiety while in stark contrast, photo-activation of CR-positive cells and photo-inhibition of Vglut2-expressing neurons produce anxiolysis. Furthermore, activation of CR- and Vglut2-positive cells provokes rearing and jumps; respectively. Our findings provide the first experimental evidence that ZI subpopulations encode and modulate different components of anxiety.
Cortical network underlying speech production during delayed auditory feedback
Authors: Ozker Muge , Doyle Werner , Devinsky Orrin , Flinker Adeen 
Published: November 2020

AbstractHearing one’s own voice is critical for fluent speech production as it allows for the detection and correction of vocalization errors in real-time. This behavior known as the auditory feedback control of speech is impaired in various neurological disorders ranging from stuttering to aphasia, however the underlying neural mechanisms are still poorly understood. Computational models of speech motor control suggest that, during speech production, the brain uses an efference copy of the motor command to generate an internal estimate of the speech output. When actual feedback differs from this internal estimate, an error signal is generated to correct the estimate and subsequent motor commands to produce intended speech. We were able to localize these neural markers using electrocorticographic recordings from neurosurgical subjects during a delayed auditory feedback (DAF) paradigm. In this task, subjects hear their voice with a time delay as they produced words and sentences (similar to an echo on a conference call), which is well known to disrupt fluency by causing slow and stutter-like speech in humans. We observed a significant response enhancement in auditory cortex that scaled with the duration of feedback delay indicating an auditory speech error signal. Immediately following auditory cortex, dorsal precentral gyrus (dPreCG), a region that has not been implicated in auditory feedback processing before, exhibited a markedly similar response enhancement suggesting a tight coupling between the two regions. Critically, response enhancement in dPreCG occurred only when subjects profoundly slowed down their speech during articulation of long utterances due to a continuous mismatch between produced speech and reafferent feedback. These results suggest that dPreCG plays an essential role in updating the internal speech estimates to maintain fluency as well as coordinating the efference copy and auditory error signals during speech production.
The regulation of liver gene expression by carbohydrates is mouse strain specific
Authors: Chi Yuling , Youn Dou Yeon , Xiaoli Alus M. , Liu Li , Pessin Jacob B. , Yang Fajun , Pessin Jeffrey E. 
Published: November 2020

AbstractC57BL/6J and BALB/cJ mouse strains were analyzed by deep mRNA sequencing of the liver in the fasted state and following ingestion of standard laboratory mouse chow supplemented with plain drinking water or water containing 20% glucose, sucrose or fructose. Supplementation with these carbohydrates induced unique extents and temporal changes in gene expressions in a strain specific manner. Fructose and sucrose stimulated gene changes peaked at 3 h postprandial, whereas glucose effects peaked at 12 h postprandial in C57BL/6J mice and at 6 h postprandial in BABL/cJ mice. Network analyses revealed that fructose changed genes were primarily involved in lipid metabolism and were more complex in C57BL/6J than in BALB/cJ mice. These data demonstrate that there are qualitative and quantitative differences in the normal physiological responses of the liver between these two strains of mice and C57BL/6J is more sensitive to sugar intake than BALB/cJ.
The structural basis of fatty acid elongation by the ELOVL elongases
Authors: Nie Laiyin , Pike Ashley C. W. , Pascoa Tomas C. , Bushell Simon R. , Quigley Andrew , Ruda Gian Filippo , Chu Amy , Cole Victoria , Speedman David , Moreira Tiago , Shrestha Leela , Mukhopadhyay Shubhashish M.M. , Burgess-Brown Nicola A. , Love James D. , Brennan Paul E. , Carpenter Elisabeth P. 
Published: November 2020

AbstractVery long chain fatty acids (VLCFAs) are essential building blocks for synthesis of the ceramides and sphingolipids required for nerve, skin and retina function and 3-keto acyl-CoA synthases (ELOVL elongases) perform the first step in the FA elongation cycle. Although ELOVLs are implicated in common diseases including insulin resistance, hepatic steatosis and Parkinson’s, their underlying molecular mechanisms are unknown. Here we report the structure of the human ELOVL7 elongase, which includes an inverted transmembrane barrel structure surrounding a 35 Å long tunnel containing a covalently-attached product analogue. The structure reveals the substrate binding sites in the tunnel and an active site deep in the membrane including the canonical ELOVL HxxHH sequence. This indicates a ping-pong mechanism for catalysis, involving unexpected covalent histidine adducts. The unusual substrate-binding arrangement and chemistry suggest mechanisms for selective ELOVL inhibition, relevant for diseases where VLCFAs accumulate such as X-linked adrenoleukodystrophy.
Common rules underlying optogenetic and behavioral modulation of responses in multi-cell-type V1 circuits
Authors: Palmigiano Agostina , Fumarola Francesco , Mossing Daniel P. , Kraynyukova Nataliya , Adesnik Hillel , Miller Kenneth D. 
Published: November 2020

AbstractThe visual cortex receives non-sensory inputs containing behavioral and brain state information. Here we propose a parallel between optogenetic and behavioral modulations of activity and characterize their impact on cell-type-specific V1 processing under a common theoretical framework. We infer cell-type-specific circuitry from large-scale V1 recordings and demonstrate that, given strong recurrent excitation, the cell-type-specific responses imply key aspects of the known connectivity. In the inferred models, parvalbumin-expressing (PV), but not other, interneurons have responses to perturbations that we show theoretically imply that their activity stabilizes the circuit. We infer inputs that explain locomotion-induced changes in firing rates and find that, contrary to hypotheses of simple disinhibition, locomotory drive to VIP cells and to SOM cells largely cancel, with enhancement of excitatory-cell visual responses likely due to direct locomotory drive to them. We show that this SOM/VIP cancellation is a property emerging from V1 connectivity structure.
Chromosome-length genome assembly and structural variations of the primal Basenji dog (Canis lupus familiaris) genome
Authors: Edwards Richard J. , Field Matt A. , Ferguson James M. , Dudchenko Olga , Keilwagen Jens , Rosen Benjamin D. , Johnson Gary S. , Rice Edward S. , Hillier LaDeanna , Hammond Jillian M. , Towarnicki Samuel G. , Omer Arina , Khan Ruqayya , Skvortsova Ksenia , Bogdanovic Ozren , Zammit Robert A. , Aiden Erez Lieberman , Warren Wesley C. , Ballard J. William O. 
Published: November 2020

AbstractBackgroundBasenjis are considered an ancient dog breed of central African origins that still live and hunt with tribesmen in the African Congo. Nicknamed the barkless dog, Basenjis possess unique phylogeny, geographical origins and traits, making their genome structure of great interest. The increasing number of available canid reference genomes allows us to examine the impact the choice of reference genome makes with regard to reference genome quality and breed relatedness.ResultsHere, we report two high qualityde novoBasenji genome assemblies: a female, China (CanFam_Bas), and a male, Wags. We conduct pairwise comparisons and report structural variations between assembled genomes of three dog breeds: Basenji (CanFam_Bas), Boxer (CanFam3.1) and German Shepherd Dog (GSD) (CanFam_GSD). CanFam_Bas is superior to CanFam3.1 in terms of genome contiguity and comparable overall to the high quality CanFam_GSD assembly. By aligning short read data from 58 representative dog breeds to three reference genomes, we demonstrate how the choice of reference genome significantly impacts both read mapping and variant detection.ConclusionsThe growing number of high-quality canid reference genomes means the choice of reference genome is an increasingly critical decision in subsequent canid variant analyses. The basal position of the Basenji makes it suitable for variant analysis for targeted applications of specific dog breeds. However, we believe more comprehensive analyses across the entire family of canids is more suited to a pangenome approach. Collectively this work highlights the importance the choice of reference genome makes in all variation studies.
A validation study of the Korean version of the thriving at work measurement
Authors: Lee Jae Young , Lee Yunsoo 
Published: November 2020

PurposeThe purpose of this study is to validate the Korean version of the thriving at work measurement by Porath et al. (2012).Design/methodology/approachAfter translating the thriving at work measurement into Korean, the researchers assessed the validity and reliability of the measurement in a Korean working context using two different samples. In Study 1, the study validated the translated measurement using Rasch’s (1960) model, exploratory factor analysis and a reliability test with a sample of 322 employees. In Study 2, the study conducted a confirmatory factor analysis, a reliability test and a convergent and discriminant validity test using a sample of 187 employees.FindingsBased on the analyses, this paper concluded that thriving at work has a two-factor construct and eight-item thriving at work measurement was better than the original 10-item measurement. The eight-item measurement demonstrated good discriminant and convergent validity.Originality/valueThis study validated the thriving at work measurement in a Korean context using Rasch’s (1960) model from the item response theory perspective.
Adapting CTF challenges into virtual cybersecurity learning environments
Authors: Karagiannis Stylianos , Magkos Emmanouil 
Published: November 2020

Purpose
This paper aims to highlight the potential of using capture the flag (CTF) challenges, as part of an engaging cybersecurity learning experience for enhancing skills and knowledge acquirement of undergraduate students in academic programs.

Design/methodology/approach
The approach involves integrating interactivity, gamification, self-directed and collaborative learning attributes using a CTF hosting platform for cybersecurity education. The proposed methodology includes the deployment of a pre-engagement survey for selecting the appropriate CTF challenges in accordance with the skills and preferences of the participants. During the learning phase, storytelling elements were presented, while a behavior rubric was constructed to observe the participants’ behavior and responses during a five-week lab. Finally, a survey was created for getting feedback from the students and for extracting quantitative results based on the attention, relevance, confidence and satisfaction (ARCS) model of motivational design.

Findings
Students felt more confident about their skills and were highly engaged to the learning process. The outcomes in terms of technical skills and knowledge acquisition were shown to be positive.

Research limitations/implications
As the number of participants was small, the results and information retrieved from applying the ARCS model only have an indicative value; however, specific challenges to overcome are highlighted which are important for the future deployments.

Practical implications
Educators could use the proposed approach for deploying an engaging cybersecurity learning experience in an academic program, emphasizing on providing hands-on practice labs and featuring topics from real-world cybersecurity cases. Using the proposed approach, an educator could also monitor the progress of the participants and get qualitative and quantitative statistics regarding the learning impact for each exercise.

Social implications
Educators could demonstrate modern cybersecurity topics in the classroom, closing further the gap between theory and practice. As a result, students from academia will benefit from the proposed approach by acquiring technical skills, knowledge and experience through hands-on practice in real-world cases.

Originality/value
This paper intends to bridge the existing gap between theory and practice in the topics of cybersecurity by using CTF challenges for learning purposes and not only for testing the participants’ skills. This paper offers important knowledge for enhancing cybersecurity education programs and for educators to use CTF challenges for conducting cybersecurity exercises in academia, extracting meaningful statistics regarding the learning impact.
A meta-analytic model on the role of organizational support in work-family conflict and employee satisfaction
Authors: Zhao Xinyuan (Roy) , Wang Jiale , Law Rob , Fan Xinping 
Published: November 2020

Purpose
This study aims to illustrate how organizational support can reduce work-family conflict (WFC) and improve job/life satisfaction by synthesizing the empirical findings among hospitality employees.

Design/methodology/approach
Previous empirical papers were searched through tourism and hospitality journals and 54 studies were ultimately selected. The correlation coefficients were coded and examined through meta-analysis, after which they were used to test the hypothesized model via meta-analytic structural equation modeling.

Findings
Findings demonstrated that organizational support plays a critical role in helping employees release WFC and improve life satisfaction but not job satisfaction. The number of children is a salient factor at the individual level on predicting WFC, whereas gender relates only to life satisfaction. The asymmetric permeable roles of WFC dimensions among work, family and life domains were also shown.

Practical implications
The findings can help hospitality managers be aware of the critical roles of organizational support in assisting employees to handle WFC and improve job and life satisfaction.

Originality/value
The relationships among organizational support, WFC and job/life satisfaction of frontline employees have been examined for the first time via meta-analytic SEM. In this manner, previous consistent and inconsistent findings can be synthesized for future theoretical development.
Legal risk management: Can the COVID-19 pandemic be included as a force majeure clause in a contract?
Authors: Januarita Ratna , Sumiyati Yeti 
Published: November 2020

Purpose
This study aims to investigate the legal consequences of the use of force majeure (FM) clause in a contract related to the prospect of business sustainability. In addition, this paper also examines the legal risk perspective toward the interpretation of FM clauses in contracts that incorporate the coronavirus disease 2019 (COVID-19) pandemic situation.

Design/methodology/approach
The research design in this paper uses the normative juridical method, which means that it is carried out with library research on secondary data in the field of law, in the form of primary and secondary legal materials and tertiary legal materials. Primary legal materials are in the form of regulations relating to the variable topics of this paper, namely, FM, COVID-19, contracts and legal risk management (LRM).

Findings
The study found that studies of FM are still limited in terms of the aspects of contract sustainability, and none has reviewed them using the LRM paradigm.

Research limitations/implications
Given the fact that this pandemic is still ongoing and uncertain, the extent to which the broadening of the interpretation of FM in the contract by the parties and how much the designs offered above can help the parties, will greatly depend on the commitment of the parties. However, if the orientation is to maintain a long-term business relationship, it still fulfills the essence of a win–win solution that will greatly assist the parties in determining the continuity of the contract.

Practical implications
The results of this study are expected to provide benefit to the parties in an agreement affected by the COVID-19 outbreak and by regulators who wish to provide a legal basis in contract law.

Social implications
Long-term business relationships will create sound, peaceful and conducive environment for modern business. This kind of situation will sustain the business as expected.

Originality/value
This study concludes that the interpretation of FM can be extended to accommodate the interests of the parties to the contract by considering several principles in contract law and other relevant clauses. In addition, this study also produced four essential designs for LRM oriented to long-term business relationships in a win–win solution.
Researching navigation of project complexity using action design research
Authors: Mikkelsen Mogens Frank , Venable John , Aaltonen Kirsi 
Published: November 2020

PurposeProject complexity is becoming increasingly challenging for project managers. Much valuable research has been done on the concept of project complexity. The research reported in this paper aims to provide a new means (the “Complexity Navigation Window”) and guiding principles for the navigation of project complexity in practice.Design/methodology/approachThis paper applied action design research (a methodology for design science research) to design and evaluate the Complexity Navigation Window (CNW), which will serve as a representation of project complexity as a key component of the user interface for a decision support system (DSS) for managing project complexity.FindingsFormative evaluations of the CNW by 16 project management practitioners indicated that the artefact is relevant, comprehensible and heading in a promising direction to guide decision-making. The evaluation also highlighted project managers' difficulty in using the (conceptual) representation by itself to assess a project's current situation accurately, which in turn limits their ability to understand a project's current complexity and decide an appropriate course of strategy. A conceptual framework by itself is insufficient. This finding motivates further research to develop and evaluate a DSS that would partially automate the assessment process (by surveying stakeholders and automatically assessing and representing project complexity according to the CNW), which should aid in increasing the accuracy (and timeliness) of project complexity assessments and contribute to appropriate strategy formulation and timely revision.Practical implicationsThe formative evaluation of the CNW indicates relevance for practitioners and the further features of the DSS may still yield even higher perceived utility from the full artefact.Originality/valueThe paper provides improved understanding of practitioners' perceptions of project complexity and ability to assess it for a given project. The paper describes the design of a new visualisation for navigating and managing complexity. The paper further presents four strategies for managing project complexity. Finally, the paper also provides a methodological discussion on the potential of ADR in advancing project management research.
Remanufacturing supply chain: an analysis of performance indicator areas
Authors: Ansari Zulfiquar N. , Kant Ravi , Shankar Ravi 
Published: November 2020

PurposeRe-use of products in the supply chain has become a significant consideration in the last decade. It has resulted in the development of several product recovery alternatives. Remanufacturing in the supply chain is one such product recovery option that yields social, economic and environmental benefits. This study aims is to identify and evaluate the key performance indicators (KPIs) of the remanufacturing supply chain (RSC).Design/methodology/approachThe KPIs of RSC are classified along with the five primary management processes (plan, source, make, deliver and return) of the supply chain operations reference (SCOR) model. A grey decision-making trial and evaluation laboratory (DEMATEL) technique is applied to investigate the complex interrelationships amongst the identified KPIs and categorize them into cause and effect group. The applicability of the proposed framework is demonstrated through a case organization involved in remanufacturing business.FindingsThe KPIs are identified based on literature analysis and subsequent discussion with decision panel experts. The present research work results reveal that “consumer awareness program”, “technological compatibility” and skilled workforce' are the most influential indicators.Originality/valueThis research work provides a framework to evaluate the causal relationship between the RSC KPIs. The framework proposed in this study is empirically applied to a case organization. Based on the study findings some important recommendations are presented to the decision-makers/policy planners to help them develop an action plan. This would help the case organization reduce resource consumption, increase market share and enable sustainable development.