bluuebunny/crossref_metadata_2025_split huggingface dataset:
Delayed Intrathoracic Gastric Perforation after G-POEM for Treating Post-Operative Refractory Gastroparesis：A Case Report and Review of Literature
Authors: Dai Shenglan , Sun Zhiyue , Shi Yan , Wang Tao , Xu Yaping , Yao Jun 
Published: September 2020

Abstract
Background: Gastric peroral endoscopic pyloromyotomy(G-POEM) is introduced as a strategy to treat post-operative refractory gastroparesis. An intrathoracic gastric perforation is a rare complication of G-POEM. Case presentation: This report described the case of a patient who suffered with an intrathoracic gastric perforation about 10 days after a G-POEM procedure. A review of the PUBMED literature indexed in English with the key words of perforation and G-POEM was performed and the results were considered. The results indicated that delayed perforation was a rare complication after G-POEM. Overeating may cause the severe intrathoracic gastric dilation and perforation. Furthermore, successful nonsurgical management after G-POEM complicated by delayed perforation could be a highly feasible option, if intensive conservative treatments were used. Conclusions: Delayed perforation after G-POEM treating post-operative refractory can lead to severe mediastinal and thoracic infections. Fully prepared before the G-POEM procedure and effective suture instrument during the procedure may reduce the incidence of perforations. If perforation occurs after G-POEM, conservative treatment can be chosen if a patient’s general situation allows. However, the choice of surgical treatment must not be ignored if the patient's overall condition deteriorates.
WITHDRAWN: Assessment of Knowledge, Attitude and Associated Factors toward COVID-19 among Nurses Who Work in South Gondar Zone, Hospitals, Northwest Ethiopia 2020, Institution-based cross-sectional Study
Authors: Feleke Dejen Getaneh , Tassew Sheganew Fetene , Chanie Ermiase Sisay 
Published: September 2020

Abstract
BackgroundCoronavirus disease 2019 is an emerging respiratory disease that is caused by a novel coronavirus and was first detected in December 2019 in Wuhan, China. The world is affected by the Corona Virus Disease in 2019. In sub-Saharan Africa Including Ethiopia there is no study conducted on level of Knowledge, Attitude and Associated Factors toward Coronavirus disease in 2019 among Health care workers Specifically Nurses.ObjectiveThis study aims to assess the level of Knowledge, Attitude and Associated Factors toward Coronavirus disease in 2019 among Nurses Who Work in South Gondar Zone, Hospitals, Northwest Ethiopia, 2020.MethodsAn Institution-based cross-sectional study was conducted among 166 Nurses in South Gondar Zone, Ethiopia, From June 1st -30/2020. To select study participants after proportional allocation of study subjects to each Hospital, simple random sampling technique were to be used. The Data was entered into Epi info version 7.2.0.1, and exported to Statistical Package for Social Sciences window version 24 for analysis. Binary and Multivariable logistic regression wasl used to see the association between dependent and independent variables. Adjusted odds ratio with 95% confidence interval was computed. P-value < 0.05 was used to declare association. Finally; the result is presented in the form of texts, tables, and graphs.ResultsOf 166 Nurses, 166 (100% response rate) responded to the online interview questionnaire. From 166 nurses, 57.2% of the participants were Females and 42.8% were males, 41.6 % of the respondents were between the ages of 20 and 29 years. About 84.9 % had good knowledge and 63.3% favorable attitude of COVID-19. Wearing general medical masks can prevent one from acquiring infection by the co vid-19 virus (AOR = 0.44, 95% CI =0.005-0.362 were factors of knowledge about COVID-19. Whereas, strongly agree Medical staffs are ready to participate in antiepidemic in the community (AOR =0.08, 95% CI = (0.003-1.76 Were factors of attitude about COVID-19.ConclusionIn this study, most of the nurses had good knowledge and favorable attitude regarding COVID-19. Wearing general medical masks can prevent one from acquiring infection by the covid-19 virus were the factors in association with knowledge OF nurses on COVID-19. Similarly, Medical staffs are ready to participate in antiepidemic in the community factors association with attitudes of nurses on COVID-19.
Molecular Detection and Identification of “Candidatus Ehrlichia hainanensis”, A novel Ehrlichia Species in Rodents from Hainan Province, China
Authors: Liu Peipei , Zhao Hongqing , Xue Feng , Gao Yunfeng , Lu Miao , Li Kun 
Published: September 2020

Abstract
Background: Ehrlichia is a genus of Rickettsiales bacteria closely related to human/animal disease and it plays an important role in public health. Naturally they are hosted by mammals such as canines, bovines, wild rodents and vectored by ticks. Results: In this study, we collected 121 rodent samples including 67 Niviventer fulvescens, 27 Rattus tanezumi, 24 Chiromyscus sp., 2 Rattus nitidus and 1 Leopoldamys edwardsi from Hainan province, which consists of the second largest island in China. The presence and genetic diversity of Ehrlichia was evaluated and characterized by amplifying and sequencing the 16S rRNA, groEL and gltA genes. An Ehrlichia was detected in 5 of the 67 Niviventer fulvescens samples (7.46%). The 16S rRNA, groEL and gltA genes show highest 99.20%, 89.87% and 83.86% identity to known Ehrlichia sequences. In the phylogenetic trees they form a distinct cluster from any other species.Conclusions: We propose that this Ehrlichia present a putative novel species nominated as ‘Candidatus Ehrlichia hainanensis’. Its pathogenicity to human is still to be further researched and molecular surveillance in local populations is needed.
An evaluation of the effectiveness of a telephone assessment and advice service within an ED Physiotherapy clinic: A single-site cohort study
Authors: Kelly Marie , Higgins Anna , Murphy Adrian , McCreesh Karen 
Published: September 2020

Abstract
BackgroundIn response to issues with timely access for musculoskeletal physiotherapy, telephone assessment and advice services have been evaluated in primary care settings. It is unclear whether this service model can reduce wait times and non-attendance rates for Emergency Department (ED) physiotherapy, compared to usual care. A secondary aim was to evaluate service user acceptability.MethodsThis was a single-site cohort study that compares data on non-attendance rates, wait time to first physiotherapy contact and participant satisfaction between patients that opted for a service based on initial telephone assessment and advice, versus routine face-to-face appointments. 116 patients were referred for ED physiotherapy over the 3-month pilot at the ED and out-patient physiotherapy department, X, Ireland. 91 patients (78%) opted for the telephone assessment and advice service, with 40% (n=36) contacting the service. 25 patients (22%) opted for the face-to-face service. Data on non-attendance rates and wait time was gathered using the hospital data reporting system. Satisfaction data was collected on discharge using a satisfaction survey adapted from the General Practice Assessment Questionnaire. Independent-samples t-test or Mann Whitney U Test was utilised depending on the distribution of the data. For categorical data, Chi-Square tests were performed. A level of significance of p ≤ 0.05 was set for this study.ResultsThose that contacted the telephone assessment and advice service had a significantly reduced wait time (median 6 days; 3 – 8 days) compared to those that opted for usual care (median 35 days; 19 – 39 days) (p ≤ 0.05). There was no significant between-group differences for non-attendance rates or satisfaction.ConclusionGiven the faster access to ED physiotherapy, without compromising on service user satisfaction, this telephone assessment and advice service, which can be introduced rapidly, could be helpful in triaging referrals and minimising face-to-face consultations in line with COVID-19 recommendations. However, a large scale study is warranted to confirm these findings.
Unmet Need for Family Planning and Associated Factors Among Married Women of Reproductive Age in Shebedino District, Southern Ethiopia, 2020
Authors: Wonago Matewos Wotango , Hibstu Desalegn Tsegaw 
Published: September 2020

Abstract
Back Ground: The Ethiopia Demographic and Health Survey, report indicates that high level with slight decrement of unmet need for family planning among married women that is, 37%, 25%, and 22% in 2000, 2011 and 2016 respectively. One of the consequences of unmet need is unwanted pregnancy with its serious squeal of induced abortion, and ultimately results in high maternal morbidity and mortality. The risk would increase in developing countries considerably (10). Therefore, this study focused on assessing magnitude of unmet need for FP and associated factors among married women of reproductive age group in Shebedino district, Sidama Zone, South Ethiopia, 2019.Methods: Community-based cross-sectional study was conducted from April 12 to 28, 2019. Data on married reproductive age groups women were collected using a pre-tested and interviewer administered structured questionnaire from 447 women using systematic random sampling technique. The data were entered using Epi Data version 3.1 and then exported in to SPSS version 20.0 Software for analysis. The result of bi-variable and multi-variable was reported by Adjusted Odd Ratio with 95% confidence. Result: A total of 447 married reproductive age group (15-49 years) women were included in the study with 100 % response rate. Fifty Six (12.5%) 95% CI: 9.5%-15.5%) women had unmet need for family planning [42 (9.4%) for spacing and 14 (3.1%) for limiting]. Age of the respondents [AOR= 6.02, CI: 2.73, 13.27], Age at first marriage [AOR= 9.3, CI: 1.9, 45.42], birth interval, [AOR= 2.9, CI, 1.3, 6.3], Number of alive children, [AOR= 9.3, CI: 1.9, 45.42], and Age at first start to use family planning to control birth (AOR= 3.69, CI: 1.44, 9.45) were identified as a positively associated factors.Conclusion: The level of unmet need for family planning in this study (12.5%) was lower than both the national (22%) and South Regional State (21%). Thus, spacing among births for at least two or more years, enforcing the law on minimum age for the first marriage, involvement of men in shaping reproductive preferences, reducing early marriage and participating relevant stakeholders to minimize mistimed and unwanted pregnancies and unmet need for family planning are recommended.
WITHDRAWN: No Increase in Rate and Severity of Flares During Pregnancy and Post-Partum Period in Pregnant Patients with Systemic Lupus Erythematosus. A Sex, Age and Disease Duration Matched Controlled Study
Authors: Louthrenoo Worawit , Trongkamolthum Thananant , Kasitatnon Nuntana , Wongthanee Antika 
Published: September 2020

Abstract
Background: Flares in pregnant patients with systemic lupus erythematosus (SLE) have shown conflicting results. This study aimed to determine the disease activity, and rate and severity of flares in pregnant SLE patients compared with the non-pregnant SLE controls. Methods: The medical records of pregnant patients in the SLE cohort were identified. Controls were non-pregnant female SLE patients who were matched for age at diagnosis and disease duration prior to conception. Disease activity was determined by mSLEDAI-2K. The definition and severity of flares followed the SELANA-SLEDAI Flare Index. The disease activity was measured from 6 months prior to conception (-6M) until termination of pregnancy or delivery.Results: Ninety pregnancies occurred from 77 patients, of whom 36.67% had active disease at the time of conception. The pregnancy group was slightly, but significantly, younger than the control group at diagnosis (21.63 ± 5.89 years vs. 24.05 ± 7.27 years, p = 0.015). The SLE disease activity (mSLEDAI-2K) score was comparable in both groups from -6M to the post-partum period, with that in the control group being slightly but significantly higher than that in the pregnancy group at conception (3.57 ± 4.28 vs. 1.91 ± 3.44, p = 0.003). Of the 90 pregnancies, the overall incidence of flares in both groups was similar during pregnancy (39 vs. 26, p = 0.070). There also was no difference in the incidence of flare during each trimester, with 19 vs.7 flares among 90 pregnancies (p = 0.588) in the 1st, 12 vs. 12 among 82 (p = 0.682) in the 2nd, and 6 vs. 7 among 71 (p = 0.266) in the 3rd trimester. The incidence of flare during the post-partum period also was similar (42 vs. 30 flares among 90 pregnancies, p = 0.091). There was no difference in the severity of flare in each trimester, overall flare during pregnancy or post-partum flare. Conclusions: This study found no difference in flare rate or severity of flares between pregnant SLE patients and non-pregnant SLE controls, who were matched by sex, age at diagnosis and disease duration prior to pregnancy.
Mesenchymal Stem Cell-Derived Extracellular Vesicles Reduce Disease Severity and Immune Responses in Inflammatory Arthritis
Authors: Kay Alasdair G , Treadwell Kane , Roach Paul , Morgan Rebecca , Lodge Rhys , Hyland Mairead , Piccinini Anna M , Forsyth Nicholas R , Kehoe Oksana 
Published: September 2020

Abstract
BackgroundNovel biological therapies have revolutionised the management of Rheumatoid Arthritis (RA) but no cure currently exists. Mesenchymal stem cells (MSCs) immunomodulate inflammatory responses through paracrine signalling via growth factors, cytokines, chemokines and extracellular vesicles (EVs) in the cell secretome; however, MSCs are still not available in the clinic. We evaluated the therapeutic potential of MSCs-derived EVs in an antigen-induced model of arthritis (AIA). MethodsEVs isolated from MSCs in normal (21% O2, 5% CO2) or hypoxic (2% O2, 5% CO2) culture or from MSCs pre-conditioned with a pro-inflammatory cytokine cocktail were applied into the AIA model. Disease pathology was assessed 3 days post arthritis induction through histopathological analysis of knee joints. Spleens and lymph nodes were collected and assessed for T cell polarisation within the immune response to AIA. Activated naïve CD4+ T cells from spleens of healthy mice were cultured with EVs or MSCs to assess deactivation capabilities. ResultsAll EV treatments significantly reduced knee-joint swelling and histopathological signs of AIA with enhanced responses to normoxic and pro-inflammatory primed EVs. Polarisation of T cells towards CD4+ helper cells expressing IL17a (Th17) was reduced when EV treatments from MSCs cultured in hypoxia or pro-inflammatory priming conditions were applied. ConclusionsHypoxically cultured EVs present a priming methodology that is as effective in reducing swelling, IL-17a expression, Th17 polarisation and T cell proliferation as pro-inflammatory priming. EVs present an effective novel technology for cell-free therapeutic translation in treating inflammatory arthritis and autoimmune disorders such as RA.
Role of GPX4-Mediated Ferroptosis in the Sensitivity of Triple Negative Breast Cancer Cells to Gefitinib
Authors: Song Xiang , Wang Xinzhao , Liu Zhaoyun , Yu Zhiyong 
Published: September 2020

Abstract
Background: Gefitinib exhibits antitumor activity in the patients with breast cancer, but the resistance to gefitinib in triple negative breast cancer (TNBC) is a new concern. Glutathione peroxidase 4 (GPX4) is a leading regulator of ferroptosis, which is of importance for the survival of TNBC cells. This study investigated GPX4-mediated ferroptosis in gefitinib sensitivity in TNBC.Methods: Gefitinib resistant TNBC cells MDA-MB-231/Gef and HS578T/Gef were constructed, and treated with lentivirus sh-GPX4 and ferroptosis inhibitor ferrostatin-1. GPX4 expression, cell viability and apoptosis were detected. Malondialdehyde (MDA), glutathione (GSH), reactive oxygen species (ROS) levels were evaluated. The levels of ferroptosis-related proteins ACSL4, PTGS2, NOX1 and FTH1 were detected. Subcutaneous tumor model was established in nude mice, and gefitinib was intraperitoneally injected. Apoptosis was detected by TUNEL staining and Ki-67 expression was detected by immunohistochemistry.Results: GPX4 was increased in gefitinib-resistant cells. After silencing GPX4, the inhibition rate of cell viability increased, the limitation of colony formation ability reduced, apoptosis rate increased, and the sensitivity of cells to gefitinib was improved. After silencing GPX4, MDA level and ROS production were significantly increased, while GSH level was decreased. Silencing GPX4 promoted ferroptosis. After inhibition of ferroptosis by ferrostatin-1, it revealed that inhibition of GPX4 promoted gefitinib sensitivity by promoting cell ferroptosis. In vivo experiments also showed that inhibition of GPX4 enhanced the anticancer effect of gefitinib through promoting ferroptosis.Conclusion: Inhibition of GPX4 stimulated ferroptosis and thus enhanced TNBC cell sensitivity to gefitinib.
Construction of An Immune-Related CeRNA Network as a Prognostic Signature in Bladder Cancer
Authors: Zhou Ran-ran , Tian Hu , Yang Cheng , Peng Fan , Yuan Hao-yu , Zhou Jun-hao , Zhi Xi , liu cundong 
Published: September 2020

Abstract
Background: Immunotherapy has been proved to be effective for bladder cancer (BLCA). However, the molecular network involved in BLCA tumor immune response remains unclear. This study aims to construct an immune-related ceRNA network and to identify the prognostic value. Methods: Based on The Cancer Genome Atlas (TCGA), we used single-sample gene set enrichment analysis (ssGSEA), weighted gene co-expression network analysis (WGCNA) to determine immune-related mRNA, lncRNA and miRNA. Then least absolute shrinkage, and selection operator (LASSO) and Cox regression were performed to identify the mRNAs with high prognostic value, and accordingly, the risk score was calculated. Internal and external validation were performed both in TCGA and GSE13507 with Kaplan-Meier (KM) survival and Receiver Operating Characteristic (ROC) curve analysis. Using the immune-related mRNA, lncRNA and miRNA, a ceRNA network was established via MiRcode, starBase, miRDB, miRTarBase and TargetScan. Besides, we also explore the relationship between the risk score and immune cell infiltration via CIBERSORT algorithm. Results: 5 mRNAs (PCGF3, FASN, DPYSL2, TGFBI and NTF3) were ultimately identified, and KM survival analysis displayed the 5-mRNA risk signature could predict the prognosis of BLCA with high efficacy both in TCGA (p = 1.006e-13) and GSE13507 (p = 7.759e-04). Using miRNA targeting molecular prediction database, an immune-related ceRNA network, including 5 mRNAs, 24 miRNAs and 86 lncRNAs, was constructed. Memory B cells, activated dendritic cells, and regulatory T cells infiltration into tumors were negatively correlated with risk score, while the infiltration levels of macrophages M0, M1 and M2 were positively correlated with risk score. Conclusion: This study helped to better understand the molecular mechanisms of tumor immune response from the view of ceRNA hypothesis, and provided a novel prognostic signature for bladder cancer.
Pathway-Specific Protein Domains (PSPD) Discrimination by Using a Hybrid Feature Space Based on Deep Neural Network(DNN)
Authors: Ghulam Ali , Lei XiuJuan , Zhang Yuchen , Wu Zhenqiang 
Published: September 2020

Abstract
The Pathway-specific protein domains (PSPDs) are important tools in examining drug growth as they provide a fast, reliable, and inexpensive way of estimating complex new molecular targets in specific diseases. The protein architecture prevents the formation of a direct correlation between signal transduction behavior and cellular structure. Accordingly, protein–tissue factor pathway inhibitor 2 isotypes 1 precursors have been used to encode peptide sequence information into specific feature structures. The measurable structure-activity classification model obtained by machine learning technology can predict pathway-specific protein interactions and new signaling peptides. We introduce deep neural network (DNN)-based PSPDs, abbreviated as DNNPSPDs, as the first pathway-specific protein domain that is built based on five extant models, namely, the AAindex, pseudo-amino acid composition, amino acid composition, composition mood of pseudoamino acids, and dipeptide composition. A total of 900 proteins with undetermined roles collected from the PDB data base are tested to evaluate the predictive power of this model. Various combinations of the available feature selection technologies are also combined to process a hybrid function space. DNNPSPDs predicts PSPDs by using features that are automatically learned from primary protein sequences. The sequences of pathway-associated proteins are sequentially fed into and decoded in neural network layers. Several classifications are also employed. DNNPSPDs achieves a prediction accuracy of 0.957 at a Matthew’s correlation coefficient (MCC) of 91.86%, with DPC, and 2nd achieve high prediction score 0.936 at Matthew’s correlation coefficient (MCC) of 88.02%, accuracy which is probably better. In terms of ROC–AUC, DNNPSPDs achieves a ROC–AUC curve of 0.982, which is larger than that of the other machine learning classifiers. A study using an alternative dataset reveals that our primary pathways, as pathway-specific protein domains, have accurate and reliable associations, thereby proving the viability of the proposed DNNPSPDs.
Rabbit thyroid extracellular matrix as a 3D bioscaffold for thyroid bioengineering
Authors: Weng Jie , Chen Bi , Xie Mengying , Wan Xinlong , Wang Peng , Zhou Xiaoming , Zhou Zhiliang , Mei Jin , Wang Liang , Huang Duping , Chen Chan , Wang Zhibin , Wang Zhiyi 
Published: September 2020

Abstract
Advances in regenerative medicine technologies have been strongly proposed in the management of thyroid diseases. Mechanistically, the adoption of thyroid bioengineering requires a scaffold that shares a similar three dimensional (3D) space structure, biomechanical properties, protein component, and cytokines to the native extracellular matrix (ECM). Herein, we prepared the 3D thyroid scaffold from the decellularization rabbit thyroid. Notably, through the imaging studies, it was distinctly evident that our protocol intervention minimized cellular materials and maintained the 3D spatial structure, biomechanical properties, ECM composition, and biologic cytokine. Consequently, the decellularization scaffold was seeded with human thyroid follicular cells, thus strongly revealing its potential in reinforcing cell adhesion, proliferation, and preserve important protein expression. Therefore, these findings revealed that the adoption of our protocol to generate a decellularized thyroid scaffold can potentially be utilized in transplantation to manage thyroid diseases through thyroid bioengineering.