bluuebunny/crossref_metadata_2025_split huggingface dataset:
Co-expression network analysis identified novel potential Signature Genes Associated with human left ventricle cardiomyopathies arises from different etiologies
Authors: Tian Jiao , Wu Zhengyuan , He Yingying , LIU SHUBAI 
Published: July 2020

Abstract
Background: Heat disease is worldwide pandemic and brings huge healthcare burden to global society. However, it is still illusive that the whole transcription disorder pattern of cardiomyopathies arises from different etiologies. We applied Weighted Gene Co-Expression Network Analysis (WGCNA) to construct and screen functional gene significantly related to 8 subtypes of cardiomyopathies. Through co-expression and protein-protein interaction (PPI) networks enrichment analysis, the hub genes and key pathways were identified, which highly correlated with pathologic traits. Compared with expression profile of healthy group, potential disease signature genes were validated through another independently investigations of cardiomyopathies via cardiovascular disease bioportal. Results: The novel potential disease signature genes were identified and assembled into three axes that shared among five cardiomyopathies groups, including idiopathic cardiomyopathy, familial cardiomyopathy, post-partum cardiomyopathy, Ischemic cardiomyopathy and viral cardiomyopathy. Four disease signature genes (MDM4, CFLAR, RPS6KB1, PKD1L2) were shared by ischemic and ischemic cardiomyopathy group. Eight signature genes (MAPK1, MAPK11, MAPK14, LMNA, RAC1, PECAM1, XIAP, CREB1) were overlapped by Ischemic Cardiomyopathy with Post. Partum/Familiar/Idiopathic Cardiomyopathy groups. The signature genes (TFAM, RHEB) were common genes among Viral Cardiomyopathy and Post. Partum / Familiar /Idiopathic Cardiomyopathy groups. These some novel signature genes were highlighted as potential biomarkers for cardiomyopathies. The majority disorder functions and pathways enriched in metabolic processes and concentrated on MAPK signaling pathway, protein processing in endoplasmic reticulum, regulation of actin cytoskeleton pathway. Conclusion: It strongly suggests that expression disorder of these signature genes may contribute the cardiac dysregulation and relapse into cardiomyopathies. Taken together, these novel signature genes could be utilized as diagnostic biomarkers or therapy targets and benefit the precise clinical diagnostics with better outcome. In summary, this study will attract great interest of clinical research scientists as well as medical scientists that work on heart diseases.
Impact of online Internet Training on the Prevention and Control of the Novel Coronavirus Pneumonia
Authors: Wang Xiaoli , Zhang Haiyan , Xu Hongmei , Wang Yao , Wang Ping 
Published: July 2020

Abstract
Background

To observe the impact of online internet training on the prevention and control of the novel coronavirus pneumonia, and to supply clinical evidence for an effective safety training model for emergent public safety situations.
Methods

A total of 2,080 medical technicians of the Third People’s Hospital of Yancheng, affiliated to Southeast University, were enrolled and divided into two groups based on the training mode (online vs. routine). They were evaluated based on the rate of excellence in five aspects: medical technicians’ prevention and control, patient management, disinfection, medical waste disposal, and hand hygiene. A questionnaire was used to check the theoretical knowledge of both groups of the prevention and control of the new coronavirus pneumonia.
Results

The online training group had a higher excellence rate for medical technicians’ prevention and control, patient management, disinfection, and medical waste disposal than the control group (p < 0.05). However, there was no statistically significant difference in hand hygiene assessment between the two groups. Based on the self-made questionnaire star-based survey, the online training group was more informed than the control group on the theoretical knowledge of the new coronavirus pneumonia.
Conclusion

Online training can effectively and safely improve the awareness regarding the infection-related knowledge and compliance of preventive measures. Thus, it should be implemented in hospitals to minimize the risk of cross-transmission among people.
Translation and Cross-Cultural Adaptation of the Questionnaire of Sleep Health for Using in Chinese Older
Authors: tang he , zhang min , liu yujin 
Published: July 2020

Abstract
Objective: to make the translation, cross-cultural adaption and content and face validation of Questionnaire of sleep health for use in Chinese elder, as well as measuring psychometric properties of the questionnaire. Methods: A methodological study carried out in six steps: translation of the instrument, consensus of the translations, back-translation, analysis by an expert committee, pre-testing and then evaluation of psychometric properties. Psychometric properties, including item analysis (the extreme group comparison and item-total correlations), content validity (item-level content validity index (I-CVI) and scale-level content validity index (S-CVI)), construct validity confirmatory factor analysis (CFA) and internal consistency (Cronbach’s α and test–retest reliability) were measured.Results: The instrument was translated, transculturally adapted and its final version consisted of 12 items. The Q-USM has excellent internal consistency (Cronbach’s α=0.76). The test–retest reliability coefficient was 0.97.The I-CVI ranged from 0.80 to 1.00, and the S-CVI was 0.98. CFA showed that the three-factor model explained 56.54% of the total variance, with a good model fit (likelihood ratio χ2/df=3.75, incremental fit index=0.86, comparative fit index=0.86, goodness-of-fit index=0.89, adjusted goodness-of-fit index=0.84, standardized root mean square error of approximation=0.09 and root mean square residual=0.02).Conclusion: The Q-USM was translated and adapted to Chinese elder, and the psychometric properties of the Chinese version of the questionnaire presented satisfactory. It’s providing assessment instruments for community elderly health. As the questionnaire is applied for the first time in China, it needs to be continuously improved.
Immediate postpartum haemorrhage at the Libreville University Hospital Centre: Epidemiological profile of women
Authors: WOROMOGO SYLVAIN HONORE , Ambounda Nathalie Ledaga , Yagata-Moussa Felicite-Emma , Ossouka Liela Agnes Okoyi , Tekem Vicky Noel Simo , Ango Eliane Okira , Kouanang Alain Jepang 
Published: July 2020

Abstract
Background

In Gabon, the frequency of maternal deaths directly related to Immediate PostPartum Haemorrhage (IPPH) is 15 to 25%, despite the different means that World Health Organization (WHO) has made available to the providers of the Emergency Obstetrical and Neonatal Care (EmONC). The objective of this study was to determine the prevalence and epidemiological characteristics of IPPH in order to improve its management and reduce the rate of maternal deaths.
Methods

An analytical retrospective study involved 42,728 records, whose data were collected using a chart collection form on the basis of information contained in partograms and other patient records. The description of the sample is made using numbers and proportions. The relationship between the etiologies of IPPH and certain characteristics of the women was established using the ORs with their 95% confidence interval. The difference was significant if p < 0.05.
Results

The prevalence of IPPH was 1.6%. The 20–39 age group was the most represented. Multiparous women accounted for 67.2%. Delivery hemorrhages accounted for 65.5% of IPPH. The main factors associated with delivery hemorrhages were multiparity (p < 0.001), preterm and postterm (p < 0.001), and child weight > 4,000 g (p < 0.001). Those associated with genital track injuries were young maternal age, multiparity (p < 0.001).
Conclusions

The prevalence of IPPH was 1.6%. The most common etiology was hemorrhage from delivery. Multiparity remains the most common risk factor and the young age of the patients. It is important to improve management through better assessment of blood loss in the immediate postpartum as well as capacity building of health providers on EmONC.
Human-Pathogenic Isolations in Stagnant Water During Peruvian El Niño-Costero Phenomenon
Authors: Moya-Sala Jeel Jr , Contreras-Pulache Hans 
Published: July 2020

Abstract
Objective: To evaluate the microbiological quality of stagnant waters in Lambayeque region, Peru during the lockdown caused by El Niño-costero phenomenon. Results: We conducted a descriptive study in four Lambayeque’s districts: (Chiclayo’s downtown, Mocupe, San José, and Pimentel). Two simultaneous samples were taken from each evaluation districts and were transported to the Hospital San Bartolome in Lima Peru for their entire microbiological analysis. We isolate of human-pathogenic microorganisms (Trichuris trichiura, Entamoeba histolytica, Staphylococcus aureus, and Salmonella typhi) that showed patterns of r-esistance to conventional first-line antimicrobials (penicillin, nalidixic acid, nitrofurantoin, and chloramphenicol). Likewise, we showed evidence of microorganisms related to the sampling site (district) and with the degree of affectation by the phenomenon (p<0.05). Our results suggests that the stagnant waters of four districts of Lambayeque presented Human-pathogenic parasites and bacteria of high-medical importance by the sudden changes in the climate through El Niño-costero phenomenon
The influence of comorbidity on the severity of COVID-19 disease: systematic review and analysis
Authors: Zaki Nazar , Mohamed Elfadil Abdalla , Ibrahim Sahar , Khan Gulfaraz 
Published: July 2020

Abstract
Background: A novel form of coronavirus disease (SARS-CoV-2) has spread rapidly across the world. This disease, originating in Wuhan, China, has become a global pandemic. What risk factors influence the severity of the disease is of considerable importance.Aim: This research is intended to offer a systematic review/meta-analysis for assessing how common clinical conditions and comorbidities correlate with COVID-19.Methodology: Two independent researchers undertook searches using Europe PMC, Google Scholar, and PubMed. In addition, a search engine was created for screening another 59,000 articles in COVID-19 Open Research Dataset (CORD-19). Screening was undertaken for any article related to comorbidity and their influence on the progress of the disease. Random-effects modeling was used to pool 95% confidence intervals (CIs) and odds ratios (ORs). The significance of all comorbidities and clinical conditions in relation to the severity of the disease were evaluated by employing feature extraction methods and machine-learning. Publication bias was assessed by employing funnel plots, and heterogeneity was tested in relation to I2.Results: The meta-analysis incorporated 12 studies covering 4101 confirmed COVID-19 patients from Chinese hospitals. The findings demonstrate that the most common comorbidities with the disease were hypertension (22.07%, OR 2.43 [95% CI: 1.71-3.45], p <0.0001), diabetes (11.34%, OR 2.27, [95% CI: 1.46-3.53], p = 0.0003), cardiovascular disease (10.76%, OR 2.89 [95% CI: 1.90-4.40], p <0.0001), and COPD (2.53%, OR 3.24 [95% CI: 1.99-4.45], p< 0.0006). No significant associations were found for disease severity with the comorbidities of kidney disease, liver disease, or cancer.The most frequently exhibited clinical symptoms were fever (74.52%, OR 1.37, 95% CI: 1.01-1.86, p = 0.04), cough (62.15%, OR 1.25, 95% CI: 0.97-1.60, p = 0.0823), myalgia/fatigue (38.77%, OR 1.31, 95% CI: 1.11-1.55, p = 0.0018), dyspnea (33.9%, OR 3.61, 95% CI: 2.57-5.06, p = <0.0001), and respiratory failure/ARDS (20.6%, OR 11.46, 95% CI: 3.24-40.56, p = 0.0002). Meta-analysis also revealed that neither the duration of the incubation period nor current smoking status associated with disease severity.Conclusion: Existing comorbidities, including COPD, cardiovascular disease, coronary heart disease, diabetes, and hypertension represent a risk of increasing the severity of the disease in COVID-19 patients.
A Two-Stage Purkinje Network for More Accurate ECG Representations
Authors: Shalaby Noha , Aguib Heba , Badran Mohamed , Elkhodary Khalil 
Published: July 2020

Abstract
In this manuscript we propose a method to generate Purkinje networks that are anatomically and physiologically plausible, for use with in-silico modeling. Purkinje networks play a fundamental role in shaping cardiac electrical activation patterns and their corresponding clinical electrocardiograms (ECGs). Despite a known variability in ventricular activation sequences, certain sites of early activation within the left and right ventricles have been identified in the literature for normal electrical excitation patterns. Nevertheless, in-vivo imaging of Purkinje networks cannot at present yield detailed information on their structure, so there is a genuine need for in-silico models that can construct Purkinje networks that are both anatomically and physiologically plausible, in particular networks that can exhibit correctly situated early activation sites in the ventricles. Of special interest to this manuscript is the method of representation of Purkinje networks by line-like electrical elements that are generated by means of a fractal-tree algorithm (1–3) to overlay the irregular endocardial surfaces. A known drawback to a direct implementation of this approach in complex geometries relates to its incorrect modeling of clinically observed ECGs and electrical activation sequences for a human heart (4). Our aim was thus to correct this deficiency by generating Purkinje networks that leverage a pre-knowledge of the location of early activation sites. At every such site we first generate a Purkinje sub-network. These sub-networks are linked together and to the bundle of His, setting up our first stage of the Purkinje network. Subsequently, we spawn a second stage to the Purkinje network from one or more tips of any given sub-network, to cover the full endocardial surface with Purkinje elements. Our resulting activation sequences and ECGs compare favorably to those of a population of 39 healthy male individuals (the PTB diagnostic database), and our corresponding mechanical markers of cardiac function also match well with the literature.
Mesonephric Adenocarcinoma Arising From The Uterine Corpus：Case Reports and Literature Review
Authors: HU Qian , Xu Qin , Deng Yan Chun , Guo Tao , Wu Li Xiu 
Published: July 2020

Abstract
Background Mesonephric adenocarcinoma (MNAC) is a rare carcinoma which arises from the mesonephric remnant of the gynecologic tract. It mainly occurs in the uterine cervix, barely locates in the uterine corpus, ovarian and vagina. To date, only a few cases of MNAC arising from of the uterine body (UB-MNAC) have been reported, and the clinicopathologic and molecular characteristics of UB-MNAC remain limited. A recent report suggested that series of UB-MNAC should be defined as Mesonephric-like adenocarcinoma carcinomas (MLAC), for they exhibited the classic morphologic features and immunophenotype of mesonephric carcinoma, but occurring outside of the cervix and without convincing mesonephric remnants. Thus, the histogenesis of UB-MNAC is not yet clear, they may originate in Müllerian tissue and exhibits the mesonephric differentiation phenotype, or arise from the mesonephric remnants in the uterine wall.Case presentation To better understand the histogenesis of UB-MNAC, we presented three UB-MNAC cases from west china second university hospital, which exhibited typical morphologic, histologic as well as the immunohistochemical characteristics of MNAC. Notably, among the three cases, two cases arising from the myometrium layer of the uterine corpus found mesonephric remnants around the tumor. By reviewing the published UB-MNAC and UB-MLAC, we found that to our knowledge ,it is the first time finding mesonephric remnants around the MNAC cells in the reported literature, except one case that found mesonephric remnants in the cervix, and the tumors of the three cases were all arising from the myometrium layer, without endometrium involved. Then we compared the clinical characteristics of the UB-MNAC cases arising from the myometrium and endometrium, and the results showed that the two subgroups had most in common in the clinical characteristics except the myometrium subgroup had a higher elevated CA125 level, and this result was in consistent with the Kaplan-Meier survival analysis, which indicated that the myometrium subgroup had a poorer prognosis than the endometrium group. But this need more data and further study such as the molecular analysis.Conclusion Though the pathogenesis of MLAC or MNAC of the uterine corpus is still under debate, according to our cases and the published literatures, We hypothesize two different pathways involved: the MNAC arising from the myometrium not affecting the endometrium may directly develop from the mesonephric remnant, the one occurred in the endometrium may not real mesonephric adenocarcinoma, but more likely arising from mesonephric transformation of Müllerian adenocarcinoma, and is better referred as MLAC. Besides, the two kinds of adenocarcinomas may have different clinical prognosis, while the MNAC arising from the myometrium may have a poorer prognosis than the MLAC originating from the endometrium, although they have identical morphologic and histologic characteristics.
Epidemiological Features and Hotspot of COVID-19 in Isfahan Province of Iran: Results of A Cohort Study
Authors: Janani Majid , Beheshti-Nia Fereshte , Ahmadi Hamzeh , Khazeni Atefeh , Yadegarafar Ghasem 
Published: July 2020

Abstract
Background and Aim: The aim of this study was to description of the epidemiological features and hotspot of COVID-19 in Isfahan province of Iran.Method: In this descriptive, retrospective cohort, multicenter study, all patients admitted to one of the hospitals or health networks of Isfahan province from 3rd February to 13th June 2020 due to RT-PCR (Reverse transcription-polymerase chain reaction) test were enrolled to study. Trained staff followed up participants for two weeks by a Telephone number, and the outcome was recorded.Result: Up to 13 June, 41,498 patients recruited and their data were analyzed; the incidence of COVID-19 was 27.5% (95% CI: 27.1, 28.2). Among the participants with the positive test, 93.2% of them, treated by outpatient basis or discharged, 2.1% were hospitalized, and the case fatality rate (CFR) was 4.8%. Khansar and Aradestan was the hotspot of COVID-19 and had the highest incidence among cities of Isfahan province. Najafabad, Khomeinishahr, and Shahinshahr&Meymeh had the highest imported cases of COVID-19 to the capital of Isfahan province, Isfahan city. Also, Charmahal & Bakhtiari, Khuzestan, and Fars provinces had the highest number of imported cases from other provinces to Isfahan city. We found that 77.3% of intra-province imported cases and about 83% of inter-province cases were imported after reducing lockdown.conclusion:The incidence and fatality of COVID-19 in Isfahan province is alarming. Inter-city and inter-provincial unnecessary travels have a high impact on the transmission and spread of the COVID-19. Applying more restrictions will prevent this from happening. It is advised to restrict the inter cities travels again by policy makers for the benefit of public health.
Reconsideration of the Source Model for the 1662 Hyuga-nada Earthquake
Authors: Yamashita Yusuke , Ioki Kei , Kase Yoshihiro 
Published: July 2020

Abstract
The 1662 Hyuga-nada Earthquake is one of the largest earthquakes to have occurred in the Hyuga-nada region, Southwest Japan, rupturing the western part of the Nankai Trough subduction zone. Strong ground motion and a large tsunami with an estimated height of at least 4–5 m were reported along the coast of Miyazaki Prefecture, Kyushu Island, with extensive damage reported across this region. Therefore, developing a more complete picture of the 1662 Hyuga-nada Earthquake will improve our understanding of tsunami risk along the Pacific coast of Southwest Japan. Here we use the most recent geophysical data from the region to propose a novel source model for the 1662 Hyuga-nada Earthquake that incorporates our current understanding of the interactions between slow earthquakes and great earthquakes. The source area in our proposed model extends from the focal region of recurrent M7-class interplate earthquakes to the region of slow earthquakes that occur at relatively shallow depths along the plate boundary. Tsunami simulations indicate that the predicted coastal tsunami heights are broadly compatible with previously reported tsunami heights. This new model suggests that the 1662 Hyuga-nada Earthquake was probably a M8-class earthquake, and that previous magnitude determinations may have underestimated the strength of this historic earthquake. However, the model uncertainty remains large, and the calculated tsunami heights for the southern coast of Miyazaki Prefecture are very high. We suggest that future verification, including an analysis of tsunami-related deposits along the coast, will be important to more accurately determine the source area of the 1662 Hyuga-nada Earthquake.
Metaproteomics reveals potential signatures of disease-specific alterations in the gut microbial proteolytic events in inflammatory bowel disease
Authors: Yan Zhixiang , Xiao Fei , He Huanhuan , Li Dan , Cong Li , He Feixiang , Lin Lu , Wu Yanyan , Yan Ru , Li Xiaofeng , Shan Hong 
Published: July 2020

Abstract
Background:

Proteolysis regulation allows gut microbes to respond rapidly to dynamic intestinal environments by fast degradation of misfolded proteins and activation of regulatory proteins. However, alterations of gut microbial proteolytic signatures under complex disease status such as inflammatory bowel disease (IBD, including Crohn’s disease (CD) and ulcerative colitis (UC)) have not been investigated. Here, we explored the microbial proteolysis landscape using two public datasets containing 623 metaproteomes from 447 fecal and 176 mucosal luminal interface (MLI) samples from IBD patients and healthy individuals using a semi-tryptic peptide centric mining approach.
Results:

Fecal metaproteome revealed a global alteration of gut microbial proteolysis signatures in IBD, while MLI metaproteome demonstrated remarkable disease-specific and location-specific alterations at taxonomic, functional, and cleavage site motif levels. The functional alterations in IBD mainly involved microbial carbohydrate transport and metabolism, oxidative stress, cell motility, protein synthesis and maturation. Altered microbial proteolysis signatures of CD and UC mainly occurred in terminal ileum and descending colon, respectively. Microbial proteolysis patterns exhibited low correlations with β-diversity and moderate correlations with microbial protease transcriptome, respectively. Human protease inhibitors and immunoglobulins were mainly negatively associated with microbial proteolysis patterns, suggesting the inhibitory effects of these host factors on gut microbial proteolysis events.
Conclusions:

Our findings highlight the complex and diverse proteolytic alterations of gut microbiome in IBD, providing a unique layer of information beyond taxonomic and proteomic abundance.