bluuebunny/crossref_metadata_2025_split huggingface dataset:
Spatial analysis of confirmed Lassa fever cases in Edo State, Nigeria, 2008 – 2014
Authors: Owoicho Samuel Amifofum , Awosanya Emmanuel , Fadugba Dorcas , Balogun Muhammad Shakir , Nguku Patrick , Ajisegiri Simon , Eugene Odigie , Olugasa Babasola 
Published: November 2019

Abstract
Background: Lassa fever (LF) is endemic and poses public health threats in Edo State. Identification of primary clusters will help prioritize public health interventions in the state. We investigated retrospective cases of LF to identify primary cluster of the disease for household exposure management. Method: We reviewed retrospective data (n = 1400) of LF case-patients at a referral hospital in Edo State from 2008 to 2014 based on World Health Organization case definition for LF. We determined primary cluster of confirmed cases on Bernoulli model and evaluated environmental factors in the primary cluster: presence of rodent deterrents, proximity of households at 2 km radius to rice farm, rice post-harvest storage facility, refuse dump, forest, hospital and main road using Multi-criteria analysis at p < 0.05. Results: Of the reviewed cases, 171 (12.2%) were confirmed case-patients. The median age of confirmed case-patients was 30 years (Q1:16.0 and Q3: 43.5). Of the confirmed case-patients, 101 (59.1%) were male. A primary spatial cluster (4.45 km radius; geographic centre at 6.717900oN, 6.243500oE) was identified in Esan West LGA. Associated environmental factors included presence of rodent deterrents (p < 0.001), proximities of households to refuse dump (p < 0.001), rice post-harvest storage facility (p = 0.01) and rice farm (p = 0.03). Conclusion: Esan West LGA was identified as a primary cluster for Lassa fever. Presence of rodent deterrents, household proximity to refuse dump sites, rice storage facilities and rice farms were associated environmental factors. We recommend improved rice post-harvest storage and use of rodent deterrents in Edo State, Nigeria. Key words: Multi-criteria analysis, Lassa fever, environmental variables, Nigeria.
Analysis of deep sequencing Exosome-microRNA expression profile from Chicken Type Ⅱ Pneumocytes derived reveals potential role of gga-miRNA-451 in inflammation
Authors: Zhao Yabo , Fu Yali , Zou Mengyun , Sun Yingfei , Yin Xun , Niu Lumeng , Peng Xiuli 
Published: November 2019

Abstract
Background: Exosomes are nanosized extracellular vesicles secreted by multiple cells in the body, including those located in the respiratory tract and lungs. They are emerging as important inflammatory mediators and can release their contents, especially microRNAs (miRNAs), to both neighboring and distal cells. Mycoplasma gallisepticum (MG) can target host cell and cause chronic respiratory disease (CRD) in chickens. Although exosomal miRNAs have been demonstrated to produce an important effect on microbial pathogenesis and inflammatory response as crucial regulatory noncoding RNAs, the mechanism by which exosomal miRNAs regulate MG-induced inflammation remains to be elucidated. Methods: the expression of exosome-microRNA derived from MG-infected chicken type Ⅱ pneumocytes (CP-Ⅱ) was screened, and the target genes and function of differentially expressed miRNAs (DEGs) were predicted. To verify the inflammatory functions of exosomal gga-miR-451 via targeting YWHAZ, Western blot, ELISA, and RT-qPCR were used in this study. Results: A total of 722 miRNAs were identified from the two exosomal small RNA (sRNA) libraries, and 279 novel miRNAs were discovered; 30 miRNAs (9 up-regulated and 21 down-regulated) were significantly changed (P＜0.05). Function annotation analysis of DEGs showed that the target miRNAs were significantly enriched in treatment group, such as cell cycle, Toll-like receptor signaling pathway and MAPK signaling pathway, etc. The results have also confirmed that gga-miR-451-absent exosomes derived from MG-infected CP-Ⅱ cells increased inflammatory cytokine production in DF-1 (chicken embryo fibroblast) cells, and Wild Type CP-Ⅱ cells-derived-exosomes displayed protective effects. Conclusion: our work suggests that exosomes from MG-infected CP-Ⅱ cells alter the dynamics of the DF-1 cells, and may contribute to pathology of the MG infection via exosomal gga-miR-451 targeting YWHAZ involving in inflammation. This could potentially be used as a biomarker for diagnostics and treatment.
Pregnant women with gestational diabetes mellitus exhibit unique features in oral microbiome
Authors: Li Xiaoqing , Zheng Jiayong , Ma Xiuling , Zhang Bing , Zhang Jinyang , Wang Wenhuan , Sun Congcong , Wang Yeping , Zheng Jianqiong , Chen Haiying , Tao Jiejing , Wang Hai , Zhang Fengyi , Wang Jinfeng , Zhang Hongping 
Published: November 2019

Abstract
Background Gestational diabetes mellitus (GDM) leads to a series of adverse pregnancy outcomes, seriously endangering the health of mothers and infants. Oral glucose tolerance test (OGTT) is the gold standard test for GDM diagnosis, but largely increases the discomfort of pregnant women, and is inconvenient to follow-up and detection. Considering that oral sampling is convenient, rapid, safe and non-invasive, the feasibility of distinguishing GDM via biomarkers from oral microbiota was evaluated in this study. Here, the 16S rRNA gene sequencing was used to compare the microbiome of saliva and dental plaque samples of 111 pregnant women, and to analyze the structure of oral microbiota in patients with GDM as well as to find effective biomarkers.Results The results showed that the microbiota of both types of oral samples in patients with GDM changed, and it was quite different from that of neither periodontitis nor dental caries. By using bacterial biomarkers from oral microbiota, GDM classification models based on SVM and random forest algorithms were constructed. For the SVM algorithm, the AUC value of the classification model constructed by the combination of dental plaque Lautropia, Neisseria and saliva Veillonella achieved 0.83. For the random forest algorithm, the maximum AUC value of the model constructed by the dental plaque Streptococcus, Eikenella, Anoxybacillus and saliva Leptotrichia, Kingella was larger than 0.90.Conclusions These findings revealed that certain bacteria of either saliva or dental plaque can accurately distinguish GDM from healthy pregnant women, which provides a potential non-invasive approach for GDM diagnosis with oral microbial markers.
Silencing of FOXG1 confers radio-sensitivity through regulating autophagy in glioma cells
Authors: Liao Wenjun , Li Churong , Yin Jun , Huang Hui , Li Baisen , Zhang Shichuan , Zhang Peng , Yang Chuan 
Published: November 2019

Abstract
Background/Aim forkhead box G1 (FOXG1) has recently been observed in many cancers, while its effect on radio-sensitivity in glioma is still unclear. In this study, we hypothesized that FOXG1 be a major players in radio-resistance of glioma as well as the underlying mechanism.Methods Immunohistochemistry (IHC) was conducted to assess FOXG1 expression in the glioma tissues and glioma-adjacent tissues. Western Blot was applied to detect the expression of autophagy-related proteins. CCK-8 and flow cytometry assays were applied to assess proliferation and apoptosis, respectively.Results The present study demonstrated that FOXG1 was highly expressed in glioma tissues. FOXG1 silencing enhances the effect of X-ray irradiation on proliferation inhibition and apoptosis of glioma cells, while FOXG1 overexpression has the opposite effect. Interestingly, the chloroquine (CQ) of autophagy inhibitor enhanced X-ray irradiation induces proliferation inhibition and apoptosis in glioma cells.Conclusions The present study suggests that FOXG1 is a pivotal molecule for circumventing radiation-induced cell death in malignant glioma cells through the regulation of autophagy and provide a target for the treatment of human brain glioma.
Differential effects of bisphosphonates and denosumab on primary breast cancer risk, potentially to be modified by statins
Authors: Stanoyevitch Alexander , Zhang Lei , Sanz Javier , Follett Robert , Bell Douglas 
Published: November 2019

Abstract
Background The risks of osteoporosis and breast cancer are increasing in elderly women, as well as menopausal status related hyperlipidemia. Bisphosphonates and denosumab, varying in drug mechanisms and therapeutic effects are recommended medications for treatment of osteoporosis. The pharmacodynamics of bisphosphonates and statin overlap in one signal pathway. We aim to assess different effects of bisphosphonates and denosumab on breast cancer, possibly modifiable by statins use. Methods This cohort study includes 97,671 women elder than 50 years without previous history of malignancy. Results Over an average of 3.6 years follow up, 3020 women have received either denosumab (n=778) or bisphosphonates (n=2326) for osteoporosis, among them, 84 (84/3020=2.8%) patients have combination medication of both. Our data demonstrated significant difference in breast cancer occurrence after first year latency between denosumab ever prescription group (12/778, 1.54%) and bisphosphonates group (12/2326, 0.52%), P=0.0046; this significance is also proved by Log-rank test (p=0.0004). Although the breast cancer risk in bisphosphonates ever use group (12/2326, 0.52%), statins ever use group (99/15287, 0.65%), hormone users for menopausal symptoms (20/7631, 0.26%) are significantly lower than control patients (1032/74867, 1.38%), p<=0.004, there is no significant difference in breast cancer risk between denosumab group and control patients (p=0.6965). Further subgroup analysis revealed that concurrent use of statins in denosumab prescribers lowered the breast cancer risk to 0.89% (2/224), but with no significantly change of breast cancer risk in bisphosphonates group (7/919, 0.762%). Conclusion Our data suggest superior protective effects of bisphosphonates over denosumab on breast cancer risk in elderly women. Statins could potentially exert breast cancer protective effect in denosumab users but no synergistic effect in patients taking bisphosphonates. Large scale study with long term follow up is needed
Study of the co-expression gene modules of non-small cell lung cancer metastases
Authors: Wang Guanghui , Bie Fenglong , Li Guangxu , Shi Junping , Zeng Yanwu , Du Jiajun 
Published: November 2019

Abstract
Background: Metastasis regularly is a marker of the disease development of cancers. Some metastatic sites significantly showed a more serious clinical outcome in non-small cell lung cancer (NSCLC). Whether they are caused by tissue-specific (TS) or non-tissue-specific (NTS) mechanisms is still unclear. Methods: Weighted Correlation Network Analysis (WGCNA) was used to identify the gene modules among the metastases of NSCLC. The clinical significance of those gene modules was evaluated with the Cox hazard proportional model with another independent dataset. Functions of each gene module were analyzed with gene ontology. Typical genes were further studied. Results: There were two TS gene modules and two NTS gene modules identified. One TS gene module (green module) and one NTS gene module (purple module) significantly correlated with survival. This NTS gene module (purple module) was significantly enriched in the epithelial-to-mesenchymal transition (EMT) process. Higher expression of the typical genes ( CA14 , SOX10 , TWIST1, and ALX1 ) from EMT process was significantly associated with a worse survival. Conclusion: The lethality of NSCLC metastases was caused by TS gene modules and NTS gene modules, among which the EMT-related gene module was critical for a worse clinical outcome.
Estrogen receptor and progesterone receptor status and their prognostic implications in pelvic high-grade serous carcinomas: A retrospective study
Authors: Zhao Ting , Gu Weiyong , Zhang Chenyun , Jiang Hongyuan , Lu Yuan 
Published: November 2019

Abstract
Background: There is mounting evidence that ovarian, tubal and peritoneal high-grade serous carcinoma (HGSC) share common origin. It was also suggested that extrauterine HGSCs may originate from endometrium. The aim of this study is to compare the estrogen receptor (ER) and progesterone receptor (PR) status among pelvic HGSCs, and to analyze the prognostic role of ER and PR in pelvic HGSCs patients, and the prognostic factors in patients with different ER or PR status Methods: In total, 283 patients diagnosed with ovarian, tubal, peritoneal, and uterine HGSC were retrospectively analyzed. All patients’ diagnosis were reviewed by a panel of gynecologists and pathologists strictly according to criteria based on lesion distribution. Results: Patients in endometrial group were older than ovarian (60.1±8.0 vs. 54.1±8.3, p=0.000) and tubal (60.1±8.0 vs. 55.8±9.5, p=0.008) group. A higher proportion of ovarian group presented with advanced stage disease than fallopian and endometrial group (73.7% vs. 47.2%, p=0.000 and 73.7% vs. 47.4%, p=0.002, respectively). PR positivity rate was much lower in peritoneal compared to ovarian group (25.0% vs. 65.6%, p=0.000). There was no difference in survival rates among four groups. Although ER and PR were not prognostic factors for 5-year overall survival (OS) and progression-free survival (PFS), the prognostic factors were different in patients of distinct ER/PR status. More chemotherapy cycles was a protective prognostic factor in ER(+) or PR(+) patients but not in ER(-) or PR(-) patients. P53 mutation was adverse prognostic factor for OS in PR(-) patients but in PR(+) patients. Conclusions: PR positivity rate were much lower in peritoneal compared to ovarian HGSC. Although ER and PR were not prognostic factors in pelvic HGSCs, prognostic factors for survival were different in patients of different ER/PR status.
Branch Retinal Vein Occlusion Causes Topographic Optic Neuropathy and Retrograde Maculopathy
Authors: Yu Siqing , Abegg Mathias , Liu Xuan , Zinkernagel Martin S. , Munk Marion R. , Ebneter Andreas 
Published: November 2019

Abstract
Background To evaluate retinal ganglion cell (RGC) loss and axonal affection in branch retinal vein occlusion (BRVO).Methods 13 eyes of 12 non-glaucomatous BRVO patients were included. Thickness of peripapillary and macular retinal nerve fiber layer (pRNFL, mRNFL), ganglion cell-inner plexiform layer (GCIPL), inner nuclear layer, outer and total retina were measured at baseline and after a mean follow-up of 14 months. We compared the thickness between the affected and their internal reference regions. Additionally, pRNFL thickness was compared between the occluded and the healthy fellow eyes in the 11 unilateral cases.Results Significant degeneration of the pRNFL was observed in the affected sector of the BRVO eyes (P < 0.01 versus reference sector and versus fellow eye). In contrast, mRNFL and GCIPL thickness showed no difference between affected and reference regions and no correlation with pRNFL thickness. Degenerative microcystic macular edema (MME) was present in 25% of the eyes with macular edema following BRVO.Conclusion Axonal degeneration occurs in pRNFL, which suggests that anterograde degeneration is the mechanism of RGC affection in BRVO. Our results emphasized the importance of pRNFL monitoring in BRVO treatment, and considering degenerative MME in persistent edema following BRVO.
Experimental Study on the Specific Heat Capacity Measurement of Water- Based Al2O3-Cu Hybrid Nanofluid by using Differential Thermal Analysis Method
Authors: Çolak Andaç Batur , Yıldız Oğuzhan , Bayrak Mustafa , Celen Ali , Dalkılıç Ahmet Selim , Wongwises Somchai 
Published: November 2019

Background:
Researchers working in the field of nanofluid have done many studies on
the thermophysical properties of nanofluids. Among these studies, the number of studies on specific
heat is rather limited. In the study of the heat transfer performance of nanofluids, it is essential to
raise the number of specific heat studies, whose subject is one of the important thermophysical properties.

Objective:
The authors aimed to measure the specific heat values of Al2O3/water, Cu/water nanofluids
and Al2O3-Cu/water hybrid nanofluids using the DTA procedure, and compare the results with
those frequently used in the literature. In addition, this study focuses on the effect of temperature and
volume concentration on specific heat.

Methods:
The two-step method was tried to have nanofluids. The pure water selected as the base
fluid was mixed with the Al2O3 and Cu nanoparticles and Arabic Gum as the surfactant, firstly mixed
in the magnetic stirrer for half an hour. It was then homogenized for 6 hours in the ultrasonic homogenizer.

Results:
After the experiments, the specific heat of nanofluids and hybrid nanofluid were compared
and the temperature and volume concentration of specific heat were investigated. Then, the experimental
results obtained for all three fluids were compared with the two frequently used correlations
in the literature.

Conclusion:
Specific heat capacity increased with increasing temperature, and decreased with increasing
volume concentration for three tested nanofluids. Cu/water has the lowest specific heat capacity
among all tested fluids. Experimental specific heat capacity measurement results are compared
by using the models developed by Pak and Cho and Xuan and Roetzel. According to experimental
results, these correlations can predict experimental results within the range of ±1%.
Ethyl Acetate and Aqueous Fractions of Ziziphus jujuba Prevent Acute Hypertension Induced by Angiotensin II in Rats
Authors: Kamkar-Del Yasamin , Mohebbati Reza , Hosseini Mahmoud , Khajavirad Abolfazl , Shafei Mohammad N. , Rakhshandeh Hassan 
Published: November 2019

Background:The effect of hydroalcoholic extract of Ziziphus jujuba (ZJ) on hypertension has been reported previously.Objective:This experiment investigates the effect of two ethyl acetate (EA, a polar and semi-polar compound) and aqueous fractions (AQ, a polar compound) of ZJ extract on cardiovascular parameters in acute hypertension induced by angiotensin II (AngII).Methods:Rats were randomly divided into following groups (n=7 in each group): 1) Control; 2) AngII (50 ng/kg); 3) Losartan (LOS, 30 mg/kg) + AngII; 4, 5) ethyl acetate fraction (EA150 and EA300 mg/kg) + AngII and 6, 7) aqueous fraction (AQ150 and AQ300 mg/kg) + AngII. Rats were treated with both fractions and LOS orally for four weeks and in the experiment day (28th) AngII intravenously injected and cardiovascular parameters (Systolic Blood Pressure (SBP), Mean Arterial Pressure (MAP) and Heart Rate (HR)) directly were recorded by a power lab system.Results:AngII could significantly increase SBP and MAP (P<0.001) and decrease HR with respect to the control and these responses were attenuated by LOS. The SBP and MAP in both doses of EA+ AngII and the higher dose of AQ fractions + AngII were significantly lower than the AngII group (P<0.05 to P<0.001). Bradycardia induced by AngII was also reduced by LOS and both fractions. The comparison of two fractions also showed that the effect of EA fraction is greater than the AQ.Conclusion:This study indicates that both fractions of the ZJ extract have a beneficial effect on hypertension. Because effect of EA was greater than AQ, we suggested that antihypertensive effects of ZJ mediated polar and nonpolar compounds.
Теплопроводность монокристаллов твердых растворов на основе ZrO-=SUB=-2-=/SUB=-, солегированных оксидами скандия, церия и иттрия
Authors: Борик М.А. , Кулебякин А.В. , Курицына И.Е. , Ломонова Е.Е. , Мызина В.А. , Попов П.А. , Милович Ф.О. , Табачкова Н.Ю. 
Published: November 2019

Abstract The thermal conductivity of single-crystal solid solutions (ZrO_2)_1 – _ x _– _ y _– _ z (Sc_2O_3)_ x (CeO_2)_ y (Y_2O_3)_ z ( x = 0.08–0.10; y = 0.005–0.01; z = 0–0.005) was measured in the temperature range 50–300 K. Phase composition and defective structure of crystals were studied.
SISTEM INFORMASI MANAJEMEN ASET SEKOLAH BERBASIS WEB
Authors: Hoiriyah . , Andriyanto . 
Published: November 2019

Adanya sarana dan prasarana merupakan penunjang kegiatan administrasi dan pembelajaran di sebuah lembaga pendidikan. Sarana dan prasarana sangat penting untuk menunjang proses kegiatan belajar mengajar serta meningkatkan <em>rating</em> sebuah lembaga pendidikan. SDN Bicorong 1 merupakan salah satu SD yang memiliki aset berupa aset bangunan, tanah dan juga benda-benda seperti peralatan untuk kegiatan belajar mengajar. Manajemen penataan aset pada SDN Bicorong 1 masih konvensional sehingga data-data aset tersebut rentan rusak bahkan hilang. Adanya kekeliruan dalam proses pencatatan serta pengelolaan aset akan mengakibatkan meningkatnya biaya operasional pengadaan aset. Oleh sebab itu Sistem Informasi Manajemen Aset Sekolah dibangun untuk mengelola dan menyimpan data-data aset sekolah. Hasil yang diperoleh dalam pembuatan aplikasi ini adalah user dapat melakukan pencatatan penerimaan materiil, pencatatan pengolaan materiil serta melaporkan seluruh data aset maupun penyusutan aset bergerak yang ada.
Ask Them: Child Participation in the Development of Educational Services
Authors: Finelli Manuel , Rocca Claudia , Marchesi Valentina , Maggi Stefania 
Published: November 2019

In accordance with Article 12 of the United Nations Convention on the Right of the Child (UNCRC), we conducted a consultation process with 113 children between four and seven years of age from five different schools in Italy. We asked children to imagine their ideal school in guided conversations, drawings, and drawing descriptions. Their responses were organised into three thematic areas: (a) school quality, (b) purposes of early childhood education, and (c) activities to include in the school curriculum. Consistent with literature on participatory research, this six-month consultation process demonstrates that even young children’s views can be useful to policy-making. Particular attention is given to the methodology.