bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multidrug-resistant Acinetobacter baumannii resists reactive oxygen species and survives in macrophages
Authors: Sato Yoshinori , Unno Yuka , Miyazaki Chizuru , Ubagai Tsuneyuki , Ono Yasuo 
Published: November 2019

AbstractWe investigated the intracellular survival of multidrug-resistant Acinetobacter baumannii (MDRAB) clinical isolates in macrophages, after phagocytosis, to determine their virulence characteristics. After ATCC 19606 and 5 clinical isolates of MDRAB were phagocytosed by mouse and human macrophages, the bacterial count of MDRAB strains, R4 and R5, increased in the mouse macrophages, 24 hours after phagocytosis. Bacterial count of the strains, R1 and R2, was almost equal 4 and 24 hours after phagocytosis. Intracellular reactive oxygen species was detected in the macrophages after phagocytosis of these bacteria. Further, the strains R1, R2, R4, and R5 showed higher catalase activity than ATCC 19606. Additionally, strains R1, R4, and R5 grew more efficiently than ATCC 19606 in the presence of H2O2, whereas growth of strains R2 and R3 was marginally more than that of ATCC 19606 in the presence of H2O2. The MDRAB clinical isolates altered the expression of TNF-α, IL-1β, IL-6, and MIP-2 mRNA induced in J774A.1 cells, 24 hours after phagocytosis. These results provide insights into the renewed virulence characteristics of MDRAB clinical isolates. Finally, tigecycline killed MDRAB phagocytosed by the macrophages more effectively than colistin, although colistin and tigecycline are both considered effective antibiotics for the treatment of MDRAB.
PIC&RUN: An integrated assay for the detection and retrieval of single viable circulating tumor cells
Authors: Kamal Mohamed , Saremi Shahin , Klotz Remi , Iriondo Oihana , Amzaleg Yonatan , Chairez Yvonne , Tulpule Varsha , Lang Julie E. , Kang Irene , Yu Min 
Published: November 2019

AbstractCirculating tumor cells (CTCs) shed from solid tumors can serve as a minimally invasive liquid biopsy for monitoring disease progression. Because CTCs are rare and heterogeneous, their biological properties need to be investigated at the single cell level, which requires efficient ways to isolate and analyze live single CTCs. Current methods for CTC isolation and identification are either performed on fixed and stained cells or need multiple procedures to isolate pure live CTCs. Here, we used the AccuCyte-RareCyte system to develop a Protocol for Integrated Capture and Retrieval of Ultra-pure single live CTCs using Negative and positive selection (PIC&RUN). The positive selection module of PIC&RUN identifies CTCs based on detection of cancer surface markers and exclusion of immune markers. Combined with a two-step cell picking protocol to retrieve ultrapure single CTCs, the positive selection module is compatible for downstream single cell transcriptomic analysis. The negative selection module of PIC&RUN identifies CTCs based on a live cell dye and the absence of immune markers, allowing retrieval of viable CTCs that are suitable for ex vivo culture. This new assay combines the CTC capture and retrieval in one integrated platform, providing a valuable tool for downstream live CTC analyses.
Deciphering the molecular specificity of phenolic compounds as inhibitors or glycosyl acceptors of β-fructofuranosidase from Xanthophyllomyces dendrorhous
Authors: Ramirez-Escudero Mercedes , Miguez Noa , Gimeno-Perez Maria , Ballesteros Antonio O. , Fernandez-Lobato Maria , Plou Francisco J. , Sanz-Aparicio Julia 
Published: November 2019

AbstractEnzymatic glycosylation of polyphenols is a tool to improve their physicochemical properties and bioavailability. On the other hand, glycosidic enzymes can be inhibited by phenolic compounds. In this work, we studied the specificity of various phenolics (hydroquinone, hydroxytyrosol, epigallocatechin gallate, catechol and p-nitrophenol) as fructosyl acceptors or inhibitors of the β-fructofuranosidase from Xanthophyllomyces dendrorhous (pXd-INV). Only hydroquinone and hydroxytyrosol gave rise to the formation of glycosylated products. For the rest, an inhibitory effect on both the hydrolytic (H) and transglycosylation (T) activity of pXd-INV, as well as an increase in the H/T ratio, was observed. To disclose the binding mode of each compound and elucidate the molecular features determining its acceptor or inhibitor behaviour, ternary complexes of the inactive mutant pXd-INV-D80A with fructose and the different polyphenols were analyzed by X-ray crystallography. All the compounds bind by stacking against Trp105 and locate one of their phenolic hydroxyls making a polar linkage to the fructose O2 at 3.6–3.8 Å from the C2, which could enable the ulterior nucleophilic attack leading to transfructosylation. Binding of hydroquinone was further investigated by soaking in absence of fructose, showing a flexible site that likely allows productive motion of the intermediates. Therefore, the acceptor capacity of the different polyphenols seems mediated by their ability to make flexible polar links with the protein, this flexibility being essential for the transfructosylation reaction to proceed. Finally, the binding affinity of the phenolic compounds was explained based on the two sites previously reported for pXd-INV.
Pulsed laser deposition of Zn(O,Se) layers in nitrogen background Pressure
Authors: Abdalla Akram , Bereznev Sergei , Spalatu Nicolae , Volobujeva Olga , Sleptsuk Natalja , Danilson Mati 
Published: November 2019

AbstractZinc oxy-selenide Zn(O,Se) is a novel material, that can replace the toxic CdS buffer layer in thin film solar cells and other optoelectronic devices. In this paper a systematic study of the structural, optical and electrical properties of Zn(O,Se) layers, grown by pulsed laser deposition under 50 mTorr of nitrogen background pressure, over a wide range of the substrate temperature, from RT to 600 °C, is reported. XRD, Raman, HR-SEM, XPS, UV-Vis techniques and Hall effect measurements have been used to investigate the structural, and optoelectronic properties of Zn(O,Se) layers. XRD analysis revealed that the polycrystalline ternary Zn(O,Se) phase formed at 500 °C. Raman analysis confirmed the formation of the polycrystalline Zn(O,Se) phase at 500 °C and an amorphous phase at substrate temperatures below 500 °C. Similarly, XPS analysis accompanied with the modified Auger parameters confirmed formation of ternary Zn(O,Se) layer at 500 °C as well. HR-SEM investigation showed the growth of homogenous, dense and adherent films onto a glass substrate. Furthermore, optical studies revealed that all prepared films are practically transparent in the visible region of the spectrum, with a band gap around 3 eV. Hall effect measurements revealed that conductivity, and electron concentration, increased by four orders of magnitude at 600 °C. It was found, that nitrogen background pressure maintained stable ratios of elemental contents in the whole range of the substrate temperature for Zn(O,Se) layers.
Technology-Enhanced Reading Therapy for People With Aphasia: Findings From a Quasirandomized Waitlist Controlled Study
Authors: Caute Anna , Woolf Celia , Wilson Stephanie , Stokes Carol , Monnelly Katie , Cruice Madeline , Bacon Katherine , Marshall Jane 
Published: November 2019

PurposeThis study investigated the effects of technology-enhanced reading therapy for people with reading impairments, using mainstream assistive reading technologies alongside reading strategies.MethodThe study used a quasirandomized waitlist controlled design. Twenty-one people with reading impairments following stroke were randomly assigned to receive 14 hr of therapy immediately or after a 6-week delay. During therapy, participants were trained to use assistive reading technology that offered a range of features to support reading comprehension. They developed skills in using the technology independently and in applying the technology to their personal reading goals. The primary outcome measure assessed reading comprehension, using Gray Oral Reading Test–Fourth Edition (GORT-4). Secondary measures were as follows: Reading Comprehension Battery for Aphasia–Second Edition, Reading Confidence and Emotions Questionnaire, Communication Activities of Daily Living–Second Edition, Visual Analog Mood Scales, and Assessment of Living With Aphasia. Matched texts were used with the GORT-4 to compare technology-assisted and unassisted reading comprehension. Mixed analyses of variance explored change between T1 and T2, when the immediate group had received therapy but the delayed group had not, thus serving as untreated controls. Pretherapy, posttherapy, and follow-up scores on the measures were also examined for all participants.ResultsGORT-4 results indicated that the immediately treated group improved significantly in technology-assisted reading following therapy, but not in unassisted reading. However, the data were not normally distributed, and secondary nonparametric analysis was not significant. The control group was unstable over the baseline, improving significantly in unassisted reading. The whole-group analysis showed significant gains in assisted (but not unassisted) reading after therapy that were maintained at follow-up. The Reading Confidence and Emotions Questionnaire results improved significantly following therapy, with good maintenance of change. Results on all other secondary measures were not significant.ConclusionsTechnology-assisted reading comprehension improved following the intervention, with treatment compensating for, rather than remediating, the reading impairment. Participants' confidence and emotions associated with reading also improved. Gains were achieved after 14 therapy sessions, using assistive technologies that are widely available and relatively affordable, meaning that this approach could be implemented in clinical practice.
Un accélérateur de particules fait parler les œuvres d’art et les objets archéologiques
Authors: Calligaro Thomas , Pacheco Claire 
Published: November 2019

Depuis sa création jusqu’à son altération, la vie d’une œuvre d’art ou d’un objet archéologique s’inscrit au cœur de sa matière. L’enjeu des sciences du patrimoine est d’interroger cette matière sur l’activité des hommes, tout en préservant ces témoins de notre histoire. Retrouver les savoir-faire du passé, comprendre le choix des matériaux employés et déterminer l’origine des matières premières en sont les principales motivations.
En révélant directement et sans dommages la composition élémentaire des matériaux anciens, l’analyse par faisceau d’ions réalisée à AGLAÉ (Accélérateur Grand Louvre pour l’Analyse Élémentaire) est parfaitement adaptée aux problématiques des sciences du patrimoine.
Nouvelles spectroscopies Raman X du carbone pour les matériaux anciens
Authors: Bergmann Uwe , Georgiou Rafaella , Gueriau Pierre , Rueff Jean-Pascal , Bertrand Loïc 
Published: November 2019

L’identification des composés à base de carbone, bien que difficile, est une source d’information essentielle dans de nombreuses études archéologiques et paléontologiques.
La diffusion Raman de rayons X est une méthode de spectroscopie sur synchrotron qui permet d’identifier des signatures organiques, de retracer l’origine chimique des systèmes étudiés et de comprendre l’altération des composés organiques dans le temps.
Cette technique, conduite de manière non destructive, dans l’air, avec une sensibilité en profondeur afin de fournir des informations non compromises par la contamination superficielle, surmonte ainsi plusieurs contraintes fondamentales à la caractérisation des matériaux organiques anciens.
Craquelures et art : le temps et la matière
Authors: Giorgiutti-Dauphiné Frédérique , Pauchard Ludovic 
Published: November 2019

On s’intéresse à la matière qui constitue une peinture d’art, c’est-à-dire une succession de couches picturales déposées sur un support. Cet ensemble est complexe de par sa composition et sa géométrie (différentes couches superposées). Il est le siège de nombreux phénomènes physiques et chimiques qui apparaissent à plusieurs moments dans la vie de l’œuvre.Différents types de dégradations altèrent ou modifient la perception du tableau au cours du temps. Les craquelures sont parmi les plus apparentes. Leur étude permet de caractériser certaines propriétés mécaniques de la matière constituant la peinture et ainsi de révéler de nombreux secrets sur l’œuvre. Le réseau de craquelures constitue donc « l’empreinte digitale d’un tableau » et en cela peut aider à authentifier des œuvres.
Quand la technologie LiDAR révèle l’ampleur de cités enfouies
Authors: Evans Damian , Pottier Christophe , Soutif Dominique 
Published: November 2019

De tout temps, nombre d’activités humaines ont durablement transformé le paysage et, pour qui sait l’interpréter, la topographie des sites constitue donc une archive précieuse des sociétés du passé. La télédétection par LiDAR a récemment révolutionné cette approche en permettant d’analyser en détail la topographie de sites de grande ampleur, même dans des zones couvertes par une végétation dense.
Cet article prend pour exemple l’application récente de cette technique à l’exploration du complexe urbain d’Angkor, au Cambodge.
Gastric mucosal devitalization improves blood pressure, renin and cardiovascular lipid deposition in a rat model of obesity
Authors: Oberbach Andreas , Schlichting Nadine , Kullnick Yvonne , Heinrich Marco , Lehmann Stefanie , Retschlag Ulf , Friedrich Maik , Fayad Lea , Dietrich Arne , Khashab Mouen A. , Kalloo Anthony N. , Kumbhari Vivek 
Published: November 2019

Abstract
Background and study aims In lieu of the drawbacks of metabolic surgery, a method of mimicking resection of the gastric mucosa could be of value to those with obesity-related cardiovascular disease (CVD). Our study aims to investigate the effect of gastric mucosal devitalization (GMD) on blood pressure (BP) and cardiovascular lipid deposition in a rat model of obesity.
Methods GMD of 70 % of the stomach was achieved by argon plasma coagulation. GMD was compared to sleeve gastrectomy (SG) and sham (SH) in a high-fat-diet-induced rat model of obesity (48 rats). At 8 weeks, we measured noninvasive BP, renin, vessel relaxation and ghrelin receptor regulation in the aorta. In addition, we quantified cardiac lipid deposition and lipid droplet deposition in cardiac muscle and aorta.
Results GMD and SG were observed to have similar reductions in body weight, visceral adiposity, and serum lipid profile compared to SH rats. GMD resulted in a significant reduction in arterial BP compared to SH. Furthermore, there were significant reductions in plasma renin activity and percentage of phenylnephrine constriction to acetylcholine at the aortic ring in GMD rats compared to SH, providing insights into the mechanisms behind the reduced BP. Interestingly, the reduced BP occurred despite a reduction in endothelial ghrelin recteptor activation. Cardiac lipid content was significantly reduced in GMD rats. Lipid deposition, as illustrated by Nile Red stain, was reduced in cardiac muscle and the aorta.
Conclusion GMD resulted in a significant improvement in BP, renin and cardiovascular lipid deposition. GMD deserves further attention as a method of treating obesity-related CVD.
Die Geschichte der Homöopathie in der Türkei
Authors: Ağaoğlu Altunay 
Published: November 2019

ZusammenfassungBis vor wenigen Jahren war die Homöopathie in der Türkei nahezu völlig unbekannt. Dank des großen Engagements deutscher Homöopathen und einem sehr starken Interesse türkischer Ärzte, Zahnärzte und Apotheker sowie Heilpraktiker gelang es in wenigen Jahren, die Homöopathie im türkischen Gesundheitssystem zu implementieren und die offizielle Ausbildung an die Universitäten zu bringen. Nur hinsichtlich der Verfügbarkeit homöopathischer Arzneimittel besteht noch ein Entwicklungsbedarf.
The perfect in English-lexifier pidgins and creoles
Authors: Hackert Stephanie 
Published: November 2019

AbstractThis study investigates the expression of perfect meanings in thirty English-lexifier pidgins and creoles or related varieties, such as African American Vernacular English or Singlish. The data were elicited with the help of sixteen sentences and a short text from Dahl’s (1985: 198–206) typological tense-aspect questionnaire. The perfective, as the perfect’s ‘anti-prototype’ (Dahl 2014: 273), is also considered. The possession of a grammaticalized perfect category is particularly frequent in West Africa, where it is likely to constitute a case of substrate influence; moreover, the gram is considerably less frequent in English-lexifier pidgins and creoles than in non-creole languages, which may be related to recent grammaticalization processes.
Plurality and definiteness in Mauritian and Haitian creoles
Authors: Déprez Viviane 
Published: November 2019

Abstract
In addition to plurality, creole plural morphemes impart an additional meaning of definiteness or specificity to the nominal expressions they mark. As of yet, there is no precise characterization either empirical or theoretical of the semantic/pragmatic dimensions they convey. Furthermore, the question of whether this added meaning is largely fixed across distinct creoles and plural morphemes, or subject to variations has never been examined. With the goal of bringing new insights intothese questions, this paper reports the results of a comparativestudy of the properties of two creole plural morphemes in two distinct French-lexifier creoles, Haitian Creole (HC) and Mauritian Creole (MC).
Besides relying on native speaker intuitions, a detailed comparative qualitative and quantitative study of the uses of these plural morphemes was conducted in a textual corpus in two adaptations of the story of the Little Prince by Antoine De St Exupery, in Haitian Creole and in Mauritian Creole respectively. The results of this comparative investigation clearly demonstrate that the use of plural morphemes in the two creoles, though similarin a number of respects, also differ quite systematically. We observe that the distinctions noted closely mirror the uses of the singular definite marker ‘la’ bv’ also argued to subtly diverge in these two creoles (Wespel 2008, Déprez 2016, in preparation). The paper analyzes this mirroring effect as a consequence of the positions that the plural morphemes come to occupy in the nominal structure and of the way the structure building operations are constrained in the different creoles. Concretely it is argued that the plural morphemes come to derivationally occupy the position of definite articles in each of the languages, and that this derivational process is obligatory in Haitian Creole due to the pronominal nature of its plural morpheme, but remains optional in Mauritian.
In vitroandin vivoevaluation of antioxidant activity ofPetasites japonicusMaxim. flower buds extracts
Authors: Hiemori-Kondo Miki , Nii Mika 
Published: November 2019

ABSTRACTThe antioxidant activity of Petasites japonicus flower buds cultivated in Tokushima, Japan, was examined in vitro and in vivo. The flower bud extracts were assayed using either oxygen radical absorbance capacity or 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity. Antioxidants in the 80% ethanol extract were investigated using online high-performance liquid chromatography-DPPH and were identified as caffeic acid, 3-O-caffeoylquinic acid, fukinolic acid, 3,4-di-O-caffeoylquinic acid, 3,5-di-O-caffeoylquinic acid, and 4,5-di-O-caffeoylquinic acid using liquid chromatography–mass spectrometry. Fukinolic acid was the most active compound based on its activity and abundance. Administering the extracts orally to ICR mice prior to iron injection significantly suppressed plasma thiobarbituric acid reactive substance (TBARS) production. Moreover, TBARS and triglyceride concentrations in the plasma of C57BL/6 mice fed with a high fat diet were also significantly decreased by the extract. The results suggest that antioxidative compounds in P. japonicus can be used in the management of oxidative stress.
Surface processing of titanium in the medium of n-hexane by ultrashort laser pulses
Authors: Kochuev D A , Chernikov A S , Khorkov K S , Chkalov R V , Voznesenskaya A A , Prokoshev V G 
Published: November 2019

Abstract
In this paper we conducted research of the surface processed by femtosecond laser radiation in the medium of n-hexane at various scanning speeds. The obtained samples were studied using a scanning electron microscope; a description of the observed effects was presented. It has been demonstrated that depending on the mode of exposure to laser radiation, it is possible to obtain microgranules and surface structuring.
Model of the microscroll structure
Authors: Bukharov D.N. , Skryabin I.O. , Arakelyan S.M. 
Published: November 2019

Abstract
In this paper, we propose a method for the formation of TiO2 microscrolls due to the folding of thin layers in an external inhomogeneous magnetic field deposited on a glass substrate during laser ablation of a titanium target in the air. The models of the initial stages of the formation and structure of the microscrew are also proposed.
Definition of brightness temperature and restoration of true temperature of laser-induced plasma channel in nitrogen at different pressures
Authors: Tarasova M A , Kochuev D A , Zhirnova S V , Chkalov R V , Chernikov A S , Prokoshev V G 
Published: November 2019

Abstract
In this paper we study the properties of laser-induced plasma channels formed by femtosecond laser radiation in nitrogen. The temperature of the plasma channels was measured using a color pyrometer that measures the brightness temperature.
Performance of Reinforced–Concrete Frame with Embedded FRP rod
Authors: Abdulrahman Ftaikhan Ahmed , Hejazi F. , Rashid Raizal S.M. , Ostovar Nima 
Published: November 2019

Abstract
Most of the researchers have mainly focused on using of FRP rod to repair, retrofit and using it as additional strength besides steel rebars in structural which have a lack of lateral loading resistance. Application of using FRP rod as the main reinforcement is still rarely used in many areas, especially in the strengthening of the Structure. In present study, nonlinear finite element models have been developed by ABAQUS software to evaluate the effect of using CFRP rod and GFRP rod instead of steel rebars on efficiency of frame to prevent the seismic effect. Also, verifying is conducted by development nonlinear finite element models using ARCS3D software and compare with Experimental Results. Numerical results show superior performance of CFRP rod in terms of the ultimate loads, the energy dissipation, the ductility index, and Plastic hinge formation, which was around 34% improvement for ultimate loads, 6.61% increasing in energy dissipation, and 1.12 for ductility. Besides, frames reinforced with CFRP bars showed different behaviour than frames reinforced with GFRP bars due to the low elastic modulus of GFRP bars, which was increased dramatically the deflection. Comparisons between the two numerical software results showed slightly different between them. Also, verifying the numerical results with previous experimental results confirmed the accuracy of the finite elements models.