bluuebunny/crossref_metadata_2025_split huggingface dataset:
Role of IL-24 in the mucosal remodeling of children with coeliac disease
Authors: Rokonay Réka , Veres-Székely Apor , Szebeni Beáta , Pap Domonkos , Lippai Rita , Béres Nóra J. , Veres Gábor , Szabó Attila J. , Vannay Ádám 
Published: January 2020

Abstract
Background
Recently, involvement of IL-19, IL-20 and IL-24 has been reported in inflammatory diseases associated with tissue remodeling. However, their impact on the pathomechanism of coeliac disease (CD) is still completely unknown.

Methods
Expression of IL19, IL20 and IL24 was measured by real-time RT-PCR, protein amount of IL-24, α smooth muscle actin (α-SMA) and fibronectin (FN) was determined by Western-blot analysis in the duodenal biopsies of therapy naive children with CD and controls. Localization of IL-24 and IL-20RB was investigated by immunofluorescent staining in the duodenal mucosa. Effect of recombinant IL-1β, TNF-α, TGF-β and IL-17 treatment on the expression of IL19, IL20, IL24 and their receptors was investigated by real-time RT-PCR in small intestinal epithelial cells (FHs74Int), in primary duodenal myofibroblasts (pdMFs) and in peripheral blood mononuclear cells (PBMCs). Effect of IL-24 on H2O2 treated FHs74Int cells and on pdMFs was measured by MTT, LDH, Annexin V assays, real-time RT-PCR and by fluorescent microscopy.

Results
We found increased level of IL-24 (3.3×, p < 0.05), α-SMA (2.4×, p < 0.05) and FN (2.3×, p < 0.05) in the duodenal mucosa and increased expression of IL19 (3.6×, p < 0.05) and IL24 (5.2×, p < 0.05) in the PBMCs of children with CD compared to that of controls. IL-1β was a strong inducer of IL24 expression of FHs74Int cells (9.9×, p < 0.05), pdMFs (552.9×, p < 0.05) or PBMCs (17.2×, p < 0.05), as well. IL-24 treatment reduced the number of apoptotic cells (0.5×, p < 0.05) and decreased the expression of inflammatory factors, including IL1A, IL6 and TNF of H2O2-treated FHs74Int cells. IL-24 decreased the proliferation (0.6×, p < 0.05) of PDGF-B treated pdMFs. Moreover, IL-24 treatment altered the morphology of pdMFs by influencing the size of the angles between stress fibers and the longitudinal axis of the cells (2.0×, p < 0.05) and the expression of cytoskeletal components, including ACTA2, ACTB, VIM, SNAI1 and SNAI2.

Conclusion
Our results suggest that IL-24 plays a significant role in the maintenance of duodenal mucosal integrity in CD.
Excessive BCAA regulates fat metabolism partially through the modification of m6A RNA methylation in weanling piglets
Authors: Heng Jinghui , Wu Zhihui , Tian Min , Chen Jiaming , Song Hanqing , Chen Fang , Guan Wutai , Zhang Shihai 
Published: January 2020

Abstract
Background
Fat percentage and distribution in pigs are associated with their productive efficiency and meat quality. Dietary branched-chain amino acids (BCAA) regulate fat metabolism in weanling piglets with unknown mechanism. It is reported that N6-methyl-adenosine (m6A) is involved in fat metabolism in mice. The current study was designed to investigate the relationship between dietary branched-chain amino acids and fat metabolism through N6-methyl-adenosine (m6A) in weanling piglets.

Methods
A total of 18 healthy crossbred weaned piglets (Duroc × Landrace × Large White, 10.45 ± 0.41 kg) were divided into 3 treatments and were fed the low BCAA dose diet (L-BCAA), the normal dose BCAA diet (N-BCAA), or the high dose BCAA (H-BCAA) diet for 3 weeks.

Results
Our results show that compared with the N-BCAA group, the L-BCAA group had higher concentration of serum leptin (P < 0.05), while the H-BCAA group had lower concentration of serum adiponectin (P < 0.05). Fatty acid synthesis in pigs from the H-BCAA group was lower than those from the N-BCAA group with the down-regulation of lipogenic genes (ACACA, FASN, PPAR-r, SREBP-1c in ventral and dorsal fat, SREBP-1c in liver) and up-regulation of lipolysis genes (HSL, ATGL, CPT-1A, FABP4 in ventral fat, HSL in liver) (P < 0.05). Similarly, fatty acid synthesis in pigs from the L-BCAA group was also lower than those from the N-BCAA group with the decrease of lipogenic genes (ACACA in ventral, ACACA and FASN in dorsal fat, ACACA, FASN, SREBP-1c in liver) and the increase of lipolysis genes (ATGL, CPT-1A CD36, FABP4 in ventral fat and HSL, ATGL, CPT-1A in dorsal fat, CPT-1A) (P < 0.05). Feeding H-BCAA diet significantly reduced total m6A levels in ventral and dorsal fat and liver tissues (P < 0.05). The decrease of total m6A is associated with down-regulation of METTL3, METTL14 and FTO in dorsal fat and METTL3 and FTO in liver (P < 0.05). Decreased m6A modification of ACACA and FASN in ventral and dorsal adipose tissues was observed in pig fed with excessive BCAA.

Conclusion
These results suggest that insufficient or excessive BCAA decreased the fat deposition by increasing lipolysis and deceasing lipogenesis in adipose and liver tissues. Dietary excessive BCAA might regulate the process of lipid metabolism partly through the m6A RNA methylation.
Development of a gp60-subtyping method for Cryptosporidium felis
Authors: Rojas-Lopez Laura , Elwin Kristin , Chalmers Rachel M. , Enemark Heidi L. , Beser Jessica , Troell Karin 
Published: January 2020

Abstract
Background
Feline cryptosporidiosis is an increasing problem, especially in catteries. In humans, close contact with cats could be a potential source of infection although the risk of contracting cryptosporidiosis caused by Cryptosporidium felis is considered to be relatively low. Sequencing of the 60-kDa glycoprotein gene is a commonly used tool for investigation of the genetic diversity and transmission dynamics of Cryptosporidium species. However, until now the sequence of gp60 from C. felis has not been available and genotyping has been limited to less discriminatory markers, such as 18S rRNA, COWP and HSP70.

Methods
We have identified the gp60 orthologue within the genome sequence of C. felis, and used the sequence to design a nested PCR for subtyping purposes. A total of 128 clinical isolates of both feline and human origin, were used to evaluate the marker.

Results
Sequence analysis revealed large variations between the different samples. The C. felis gp60 lack the characteristic serine-tract found in many other cryptosporidian orthologues, instead it has an insertion of variable length (361–742 nt). Also, two cases of suspected zoonotic transmission of C. felis between cats and humans were successfully confirmed.

Conclusions
We have identified the gp60 gene in C. felis and show how this highly variable marker can be used in epidemiological investigations.
Inclusion of MCQs written by radiology residents in their annual evaluation: innovative method to enhance resident’s empowerment?
Authors: Amini Nadia , Michoux Nicolas , Warnier Leticia , Malcourant Emilie , Coche Emmanuel , Vande Berg Bruno 
Published: January 2020

Abstract
Aim
We hypothesized that multiple-choice questions written by radiology residents (MCQresident) for their weekly case presentations during radiology staff meetings could be used along with multiple-choice questions written by radiology teachers (MCQteacher) for their annual evaluation. The current prospective study aimed at determining the educational characteristics of MCQresident and at comparing them with those of MCQteacher.

Methods
Fifty-one radiology residents of the first to the fifth year of training took the 2017 exam that contained 58 MCQresident and 63 of MCQteacher. The difficulty index, the discrimination power, and the distractor’s quality were calculated in the two series of MCQs and were compared by using Student t test. Two radiologists classified each MCQ according to Bloom’s taxonomy and frequencies of required skills of both MCQ series were compared.

Results
The mean ± SD difficulty index of MCQresident was statistically significantly higher than that of MCQteacher (0.81 ± 0.1 vs 0.64 ± 0.2; p < 0.0001). The mean ± SD discrimination index of MCQresident was statistically significantly higher than that of MCQteacher (0.34 ± 0.2 vs 0.23 ± 0.2; p = 0.0007). The mean number of non-functional distractors per MCQresident was statistically significantly higher than that per MCQteacher (1.36 ± 0.9 vs 0.86 ± 0.9; p = 0.0031). MCQresident required recalling skills more frequently than MCQteacher which required more advanced skills to obtain a correct answer.

Conclusions
Educational characteristics of MCQresident differ from those of MCQteacher. This study highlights the characteristics to optimize the writing of MCQs by radiology residents.
Scaffold vascularization method using an adipose-derived stem cell (ASC)-seeded scaffold prefabricated with a flow-through pedicle
Authors: Dębski Tomasz , Kurzyk Agata , Ostrowska Barbara , Wysocki Juliusz , Jaroszewicz Jakub , Święszkowski Wojciech , Pojda Zygmunt 
Published: January 2020

Abstract
Background
Vascularization is important for the clinical application of tissue engineered products. Both adipose-derived stem cells (ASCs) and surgical prefabrication can be used to induce angiogenesis in scaffolds. Our aim was to compare the angiogenic potential of ASC-seeded scaffolds combined with scaffold prefabrication with that of non-seeded, non-prefabricated scaffolds.

Methods
For prefabrication, functional blood vessels were introduced into the scaffold using a flow-through pedicle system. ASCs were isolated from rat fat deposits. Three-dimensional-printed cylindrical poly-ε-caprolactone scaffolds were fabricated by fused deposition modelling. Three groups, each containing six rats, were investigated by using non-seeded, ASC-seeded, and osteogenic induced ASC-seeded scaffolds. In each group, one rat was implanted with two scaffolds in the inguinal region. On the right side, a scaffold was implanted subcutaneously around the inferior epigastric vessels (classic prefabrication group). On the left side, the inferior epigastric vessels were placed inside the prefabricated scaffold in the flow-through pedicle system (flow-through prefabrication group). The vessel density and vascular architecture were examined histopathologically and by μCT imaging, respectively, at 2 months after implantation.

Results
The mean vessel densities were 10- and 5-fold higher in the ASC-seeded and osteogenic induced ASC-seeded scaffolds with flow-through prefabrication, respectively, than in the non-seeded classic prefabricated group (p < 0.001). μCT imaging revealed functional vessels within the scaffold.

Conclusion
ASC-seeded scaffolds with prefabrication showed significantly improved scaffold vasculogenesis and could be useful for application to tissue engineering products in the clinical settings.
Correction to: BM-MSC-derived exosomes alleviate radiation-induced bone loss by restoring the function of recipient BM-MSCs and activating Wnt/β-catenin signaling
Authors: Zuo Rui , Liu Minghan , Wang Yanqiu , Li Jie , Wang Wenkai , Wu Junlong , Sun Chao , Li Bin , Wang Ziwen , Lan Weiren , Zhang Chao , Shi Chunmeng , Zhou Yue 
Published: January 2020

The original article [1] contains an error in Fig. 5 whereby sub-Fig. 5c, d & 5e are mistakenly mixed-up.
Mesenchymal stem cell-derived exosomes ameliorate cardiomyocyte apoptosis in hypoxic conditions through microRNA144 by targeting the PTEN/AKT pathway
Authors: Wen Zhuzhi , Mai Zun , Zhu Xiaolin , Wu Tao , Chen Yangxin , Geng Dengfeng , Wang Jingfeng 
Published: January 2020

Abstract
Background
A growing body of evidence suggests that stem cell-derived exosomal microRNAs (miRNAs) could be a promising cardioprotective therapy in the context of hypoxic conditions. The present study aims to explore how miRNA-144 (miR-144), a miRNA contained in bone marrow mesenchymal stem cell (MSC)-derived exosomes, exerts a cardioprotective effect on cardiomyocyte apoptosis in the context of hypoxic conditions and identify the underlying mechanisms.

Methods
MSCs were cultured using the whole bone marrow adherent method. MSC-derived exosomes were isolated using the total exosome isolation reagent and confirmed by nanoparticle trafficking analysis as well as western blotting using TSG101 and CD63 as markers. The hypoxic growth conditions for the H9C2 cells were established using the AnaeroPack method. Treatment conditions tested included H9C2 cells pre-incubated with exosomes, transfected with miR-144 mimics or inhibitor, or treated with the PTEN inhibitor SF1670, all under hypoxic growth conditions. Cell apoptosis was determined by flow cytometry using 7-ADD and Annexin V together. The expression levels of the miRNAs were detected by real-time PCR, and the expression levels of AKT/p-AKT, Bcl-2, caspase-3, HIF-1α, PTEN, and Rac-1 were measured by both real-time PCR and western blotting.

Results
Exosomes were readily internalized by H9C2 cells after co-incubation for 12 h. Exosome-mediated protection of H9C2 cells from apoptosis was accompanied by increasing levels of p-AKT. MiR-144 was found to be highly enriched in MSC-derived exosomes. Transfection of cells with a miR-144 inhibitor weakened exosome-mediated protection from apoptosis. Furthermore, treatment of cells grown in hypoxic conditions with miR-144 mimics resulted in decreased PTEN expression, increased p-AKT expression, and prevented H9C2 cell apoptosis, whereas treatment with a miR-144 inhibitor resulted in increased PTEN expression, decreased p-AKT expression, and enhanced H9C2 cell apoptosis in hypoxic conditions. We also validated that PTEN was a target of miR-144 by using luciferase reporter assay. Additionally, cells treated with SF1670, a PTEN-specific inhibitor, resulted in increased p-AKT expression and decreased H9C2 cell apoptosis.

Conclusions
These findings demonstrate that MSC-derived exosomes inhibit cell apoptotic injury in hypoxic conditions by delivering miR-144 to cells, where it targets the PTEN/AKT pathway. MSC-derived exosomes could be a promising therapeutic vehicle to facilitate delivery of miRNA therapies to ameliorate ischemic conditions.
Identification of ireA, 0007, 0008, and 2235 as TonB-dependent receptors in the avian pathogenic Escherichia coli strain DE205B
Authors: Zhang Zhonghua , Jiang Shan , Liu Yun , Sun Yu , Yu Peixin , Gong Qianwen , Zeng Hang , Li Yihao , Xue Feng , Zhuge Xiangkai , Ren Jianluan , Dai Jianjun , Tang Fang 
Published: January 2020

AbstractAvian pathogenic Escherichia coli (APEC), a pathotype of extraintestinal pathogenic E. coli, causes one of the most serious infectious diseases of poultry and shares some common virulence genes with neonatal meningitis-associated E. coli. TonB-dependent receptors (TBDRs) are ubiquitous outer membrane β-barrel proteins; they play an important role in the recognition of siderophores during iron uptake. Here, in the APEC strain DE205B, we investigated the role of four putative TBDRs—ireA, 0007, 0008, and 2235—in iron uptake. Glutathione-S-transferase pulldown assays indicated that the proteins encoded by these genes directly interact with TonB. Moreover, the expression levels of all four genes were significantly upregulated under iron-depleted conditions compared with iron-rich conditions. The expression levels of several iron uptake-related genes were significantly increased in the ireA, 0007, 0008, and 2235 deletion strains, with the upregulation being the most prominent in the ireA deletion mutant. Furthermore, iron uptake by the ireA deletion strain was significantly increased compared to that by the wild-type strain. Moreover, a tonB mutant strain was constructed to study the effect of tonB deletion on the TBDRs. We found that regardless of the presence of tonB, the expression levels of the genes encoding the four TBDRs were regulated by fur. In conclusion, our findings indicated that ireA, 0007, 0008, and 2235 indeed encode TBDRs, with ireA having the most important role in iron uptake. These results should help future studies explore the mechanisms underlying the TonB-dependent iron uptake pathway.
Dynamical analysis of a fractional-order eco-epidemiological model with disease in prey population
Authors: Moustafa Mahmoud , Mohd Mohd Hafiz , Ismail Ahmad Izani , Abdullah Farah Aini 
Published: January 2020

AbstractA fractional-order eco-epidemiological model with disease in the prey population is formulated and analyzed. Mathematical analysis and numerical simulations are performed to clarify the characteristics of the proposed fractional-order model. The existence, uniqueness, non-negativity and boundedness of the solutions are proved. The local and global asymptotic stability of all equilibrium points are investigated. Finally, numerical simulations are conducted to illustrate the analytical results. The occurrence of Hopf bifurcations and transcritical bifurcations for the fractional-order eco-epidemiological model are demonstrated. It is observed that the fractional order has a stabilization effect and it may help to control the coexistence between susceptible prey, infected prey and predator populations.
Existence and global exponential stability of anti-periodic solutions for quaternion-valued cellular neural networks with time-varying delays
Authors: Li Yongkun , Xiang Jianglian 
Published: January 2020

AbstractIn this paper, we are concerned with a class of quaternion-valued cellular neural networks with time-varying transmission delays and leakage delays. By applying a continuation theorem of coincidence degree theory and the Wirtinger inequality as well as constructing a suitable Lyapunov functional, sufficient conditions are derived to ensure the existence and global exponential stability of anti-periodic solutions via direct approaches. Our results are completely new. Finally, numerical examples are also provided to show the effectiveness of our results.
The politics of energy landscapes: the influence of local anti-wind initiatives on state policies in Saxony, Germany
Authors: Lintz Gerd , Leibenath Markus 
Published: January 2020

AbstractBackgroundIn recent years, landscapes in many countries have been transformed by efforts to fight global warming, specifically the shift towards renewable energies such as wind power. This development has been met by growing opposition from local citizens and their initiatives. There is an ongoing debate about whether and how such protests have a real impact on the development of energy landscapes.MethodsDrawing on the rare case of a state government scaling back ambitious targets for the expansion of renewables, the authors were able to analyse the impact of protests in the context of regional spatial planning and, particularly, state policy-making on energy. Conceptually drawing on the Advocacy Coalition Framework and using the method of causal-process tracing, the qualitative study investigates related interactions and decision-making processes in the federal state of Saxony, Germany, between 2011—when targets were increased—and 2013—when targets were scaled back.ResultsThe findings show that the protests grew significantly when the raised expansion targets for renewables were translated into a higher number of potential wind farm sites. The protests, which primarily referred to landscapes, were well-organised and creative. Activists worked through a range of channels across various contexts and several levels of the politico-administrative system, in particular approaching regional spatial planners and a wide range of politicians. In the end, the protests (along with other factors) significantly contributed to the readjustment by the Saxon coalition government of its energy and climate policy and thus to a slowdown of energy-related landscape change.ConclusionsThe study confirms that the influence of protesters can greatly exceed the previously studied participation in the planning of sites and the approval process for individual wind farms. Also highlighting other relevant co-influential factors, the paper contributes to the development of a middle-range theory of the impact of local opposition to wind power in the context of energy landscapes.
Mechanical and physio-biological properties of peptide-coated stent for re-endothelialization
Authors: Bae In-Ho , Jeong Myung Ho , Park Dae Sung , Lim Kyung Seob , Shim Jae Won , Kim Mun Ki , Park Jun-Kyu 
Published: January 2020

Abstract

Background
The aim of this study was to characterize the mechanical and physio-biological properties of peptide-coated stent (PCS) compared to commercialized drug-eluting stents (DESs).

Methods
WKYMVm (Trp-Lys-Tyr-Met-Val-D-Met), a stimulating peptide for homing endothelial colony-forming cell was specially synthesized and coated to bare metal stent (BMS) by dopamine-derived coordinated bond. Biological effects of PCS were investigated by endothelial cell proliferation assay and pre-clinical animal study. And mechanical properties were examined by various experiment.

Results
The peptide was well-coated to BMS and was maintained and delivered to 21 and 7 days in vitro and in vivo, respectively. Moreover, the proliferation of endothelial cell in PCS group was increased (approximately 36.4 ± 5.77%) in PCS group at 7 day of culture compare to BMS. Although, the radial force of PCS was moderated among study group. The flexibility of PCS was (0.49 ± 0.082 N) was greatest among study group. PCS did not show the outstanding performance in recoil and foreshortening test (3.1 ± 0.22% and 2.1 ± 0.06%, respectively), which was the reasonable result under the guide line of FDA (less than 7.0%). The nominal pressure (3.0 mm in a diameter) of PCS established by compliance analysis was 9 atm. The changing of PCS diameter by expansion was similar to other DESs, which is less than 10 atm of pressure for the nominal pressure.

Conclusions
These results suggest that the PCS is not inferior to commercialized DES. In addition, since the PCS was fabricated as polymer–free process, secondary coating with polymer-based immunosuppressive drugs such as –limus derivatives may possible.
First confirmed record of Sparid Pagellus bogaraveo (Brünnich, 1768) in the Syrian marine waters (Levantine Basin)
Authors: Saad Adib , Masri Mai , Sabour Waad 
Published: January 2020

AbstractThis paper aims to present the first record of Pagellus bogaraveo (Brünnich, 1768) from Syria marine waters. One specimen was caught by trawl nets at about 300 m depth in Rass Albassit, north of Lattakia, on 25 February 2019, and 6 additional specimens were caught by gill nets at a depth of 120 m off Jablah coast on 7 April 2019. This record represents the first sighting of this immigrant Atlantic species introduced in the Eastern Mediterranean (Levantine Basin). These specimens were found mixing in the same net haul with populations other Sparidae species such as: Pagellus acarne and Dentex macrophthalmus.