bluuebunny/crossref_metadata_2025_split huggingface dataset:
Experiencia de 1O años con el manejo de la colpocistouretropexia suprapúbica
Authors: Lomanto Morán Antonio , Sánchez Angarita Jacinto , Jiménez M. Pedro , Guerra G. Lucio , Rozo R. Pedro 
Published: May 2020

Se analizaron 500 casos de I.U.E. en la mujer, mediante la Colpocistouretropexia suprapúbica, entre el 1 de julio de 1977 y el 30 de junio de 1986, en los Servicios de Ginecología y Urología del Instituto Materno Infantil y el Centro Hospitalario San Juan de Dios, y de Ginecología de la Clínica Fray Bartolomé de las Casas de Bogotá.La frecuencia de la Colpocistouretropexia fue de 3.8%. El 56.2% presentaba IUE de Grado II y el 43.8% Grado III. El 96% tenía celes concomitantes discriminados así: Grado I 34.8%, Grado II 54.4% y Grado III 6.8%. El 12.4% tenía antecedente de cirugía de Kelly y el l% de Colpocistouretropexia suprapúbica.Se practicaron los siguientes exámenes paraclínicos: Urocultivo: 100%, Citología vaginal: 93.7%, Citoscopia: 100%, Urografía: 40%, Urodinamia: 11.2%, Cistometría: 19.6%, Cistografía: 14%. Se practicaron los siguientes procedimientos quirúrgicos:Suprapúbicos: 35.6%, Suprapúbico + Colporrafia Posterior: 49.2%. Suprapúbico + Histerectomía Abdominal: 12%- Suprapúbico + Pomeroy 1.8%. Suprapúbico + Cirugía Vaginall.6%. El tiempo quirúrgico fue de 1 hora 45 minutos. En el 54% se utilizó la incisión de Pfannestiel. En el-Y,.8% se utilizó material no absorbible. En el 87.14% se utilizó drenes. En el 51.8% las intervenciones fueron realizadas por Residentes.Las complicaciones intra-operatorias fueron: Ruptura vesical 1.6% -Hematuria 8.4% -Hemorragia 5.2%. Las complicaciones post-operatorias fueron: Infección urinaria 16.8%-Infección de la herida 9%- Retención urinaria 16.8%-Dolor suprapúbico 11.14%. El promedio de permanencia de la sonda dude 4.8%. La estancia hospitalaria fue de 32.8%, a dos años 24.8% y de 3 a 5 años 31%. La eficacia fue del 96.4%. Con base en la experiencia durante 10 años en nuestros servicios, consideramos que la Colpocistouretropexia suprapúbica es un procedimiento eficiente en el manejo de la IUE en la mujer sin desconocer la morbilidad que conlleva.
Estudio de embarazo en adolescentes en 11 instituciones colombianas
Authors: Pardo V. Francisco , Uriza G Germán 
Published: May 2020

En Colombia se ha venido trabajando en el campo de la salud reproductiva desde hace varios años siendo en la actualidad el área de la Adolescencia prioritaria para estudios que exploren la conducta sexual de nuestros jóvenes y el aumento cada vez mayor del embarazo en esta época de la vida.
Incidencia de hepatitis B en el servicio de ginecología y obstetricia del Hospital Militar Central
Authors: De la Cruz S. Jairo , Orejarena S. Jaime , Carmona P. Vicente 
Published: May 2020

Se analizaron mediante la técnica de ELISA 224 muestras de sangre tomadas a pacientes que concurrieron a consulta en el servicio de obstetricia y ginecología del Hospital Militar Central con el fin de establecer la incidencia de positividad para antígeno de superficie de la hepatitis B. Se establecieron dos grupos que incluían pacientes con factores de riesgo y sin ellos; en el total de las muestras analizadas siete pacientes presentaron el título positivo (3.6%), tres de ellas no a cusaron factores de riesgo, dos pertenecían al grupo de personal de salud, una había sido transfundida y una era drogadicta. La finalidad al establecer la incidencia de títulos positivos es ofrecer a los recién nacidos de estas madres, la oportunidad de recibir una inmunoprofilaxis pasiva y activa con el objeto de prevenir la adquisición de la enfermedad.
Incidencia de parto pretérmino y factores de riesgo
Authors: Monterrosa Castro Alvaro 
Published: May 2020

Para determinar la incidencia y los factores de riesgo de parto pretérmino se estudiaron todas las pacientes que en 1988 tuvieron parto prematuro en el Hospital de Maternidad Rafael Calvo, Cartagena, Colombia. Igual número de pacientes con parto de término fueron colocadas como control. 10.550 partos, 9.787 de término y 763 de pretérmino, establecen una incidencia del 7.3%. La primera causa determinada fue ruptura prematura de membranas (22.9%) y la segunda, infección urinaria (13.2%). Hemorragia de segunda mitad de gestación: 14.7%, embarazo múltiple 4.7%.En adolescentes Rr = 3 y mayores de 36 años Rr = 2. El 40% de las pacientes eran primigestantes. Parto prematuro previo Rr = 2.8. Aborto previo Rr = 4.5, falta de control prenatal Rr = 8.5, hábito de fumar Rr = 2.2, actividad laboral materna Rr = 1.9. Se hace énfasis en la importancia de determinar la presencia de diversos factores de riesgo para adelantar prevención en el desencadenamiento del trabajo de parto. La prevención· éS--e! arma más importante para combatir la prematurez. (Rev Col Obstet Ginecol 1991; 42(3): 199-2017).
Sporanox en la candidiasis vaginal
Authors: Daunas José A. , Sarmiento Alvaro Orlando 
Published: May 2020

Pacientes con candidiasis vaginal fueron seleccionadas a participar en un estudio multicéntrico abierto para evaluar el SPORANOX (ITACONAZOL Janssen) un día de tratamiento (dos cápsulas 100 mg tomadas en la mañana y en la noche del mismo día) para evaluar el flujo y la sintomatología vulvar y vaginal (eritema, edema, prurito); se realizó test de KOH y cultivo a la admisión, una semana después de tratamiento y a las cuatro semanas. Los resultados indican una mejoría significativa de la sintomatología del flujo en la primera semana de observación y 96.5% en la última semana. Los test del laboratorio y cultivo indican que 311 de 368 pacientes fueron negativas en el 85% después de la primera semana. Al final de la observación clínica hecha en 266 pacientes incluían cultivos de control de los cuales 240 fueron negativos, indicando una rata de curación del 92% . No se detectaron efectos colaterales. (Rev Col Obstet Ginecol 1991; 42(3): 219-221).
Requiem por la atención de la salud de la mujer no embarazada
Authors: Rizo Gil Alberto 
Published: May 2020

A partir de finales de 1987 el Ministerio de Salud Pública de Colombia introdujo modificaciones al Sistema de Programación de Servicios de Salud: se reajustó el modelo de programación para mejorar la atención de la demanda de la morbilidad y para reducir la mortalidad a niveles más bajos de los registrados en el año inmediatamente anterior.
Factors predicting the oral health behaviors of the Iranian students in the District 1 Tehran, Iran
Authors: Pourhaji Fatemeh , Delshad Mohammad Hossein , Tavafian Sedigheh Sadat , Hidarnia Alireza , Niknami Shamsodin 
Published: May 2020

Aim: The purpose of this examination is determining the predictors of oral health behaviors among Iranian students in district 1 Tehran based on the health belief model with added commitment to plan construct. Methods: This cross-sectional study was conducted on 351 four grade female students in the first district of Tehran, Iran in 2017. The multi‑stage random cluster sampling method was used to recruit students. The inclusion criteria were being in four – graded level of elementary schools of the 1st district in Tehran, being female students aged between 9-11 years and being physically and psychologically healthy student. Logistic regression analysis was used to identify the variables that predict oral health behaviors. Results: Totally, (N= 31.8%) students reported that they were brushing less than twice a day and (N= 55.2% ) students claimed using of dental floss once a week or less than once a day. The results indicated that perceived self-efficacy (OR=1.46, 95% CI=0.57-3.78, P<0.001), commitment to plan (OR=1.13, 95% CI=1.04-1.23, P<0.001) and cues to action (OR=1.42, 95% CI=1.14–1.76, P=0.002) were the significant predicting variables of brushing twice a day, and use of dental floss once a day or more (OR=1.02, 95% CI=0.23-3.53, P=0.003). Conclusion: This study has shown the effectiveness of the health belief model with added commitment to plan construct to predict oral health behavior in female students. Thus, it seems that the model as a acceptable framework for designing training programs to improve oral health behavior in students.
Expression and Purification of Active Human Kinases Using Pichia pastoris as A General-Purpose Host
Authors: Aziz May H. Abdel , Fan Yao , Liu Lijun , Moasser Mark , Fu Haian , Jura Natalia , Arkin Michelle R. 
Published: May 2020

Abstract
Background: The heterologous expression of human kinases in good purity and in a monomeric, soluble and active form can be challenging. Most of the reported successful attempts are carried out in insect cells as a host. The use of E. coli for expression is limited to a few kinases and usually is facilitated by large solubility tags that can limit biophysical studies and affect protein–protein interactions. In this report, we evaluate the methylotrophic yeast Pichia pastoris (P. pastoris) as a general-purpose host for expression of human kinases. Methods: Six diverse kinases were chosen due to their therapeutic importance in human cancers. Tested proteins include serine/threonine kinases cyclin-dependent kinases 4 and 6 (CDK4 and 6) and aurora kinase A (AurKA), receptor tyrosine kinase erbB-2 (HER2), and dual specificity kinase mitogen-activated protein kinase kinase 3 (MKK3b). Noting that positively charged kinases expressed with higher yield, we sought to improve expression of two challenging targets, CDK6 and HER2, by fusing the highly basic, N-terminal domain of the secreted tyrosine-protein kinase VLK. A standard expression procedure was developed for P. pastoris, followed by purification using affinity chromatography. Purity and activity of the proteins were confirmed and compared to published values. Results: Some kinases were purified with good yield and purity and with comparable activity to commercially available versions. Addition of the VLK domain improved expression and decreased aggregation of CDK6 and HER2. Conclusions: P. pastoris is a promising host for expression of soluble and active human kinases.
Associations of Demographic, Clinical and Laboratory Profiles of Patients with type II Diabetes Mellitus with its Microvascular Complications in Bangladesh
Authors: Rashid Mamunur , Mukit Abdul , Ahmed Khawza Iftekhar Uddin , Mostafa Raqibul , Parveen Sharmin , Mahbub Md. Shahriar , Khandoker Ahsan H 
Published: May 2020

Abstract
Objective: The aim of this study was to investigate the associations of makers from demographic, clinical, and laboratory profiles with microvascular complications in a cohort of type II diabetic population in Bangladesh. Methods: In this cross-sectional study 103 participants [47 Men and 56 Women; Age 40~70 years] having type II diabetes for more than 10 years were randomly recruited during their routine visits to a major Diabetic Hospital in Dhaka, Bangladesh. The associations of prevalence of microvascular complications [Cardiac Autonomic Neuropathy(CAN), Diabetic Peripheral Neuropathy(DPN), Diabetic Nephropathy(Nep), Diabetic Retinopathy(Ret)] with demographic, clinical, and laboratory profiles were assessed by multivariate logistic regression (p<0.05 and Odds ratio (OR)> 1.0). Results: The prevalence rates of CAN, DPN, Ret and Nep were 68%, 43.69%, 6.8%, and 4.8% respectively. The overall prevalence of diabetic-associated microvascular complication is 94.36% in this cohort of diabetic patients. BMI (p=0.0330, OR=1.90) and HbA1c (p=0.0535, OR=3.08) were found to be the most significant risk factors in the development of all microvascular complications. However, HbA1c for CAN; HbA1c, Microalbuminuria and Urinary creatinine for DPN; years of Diabetes, Systolic blood pressure and albumin creatinine ratio for Nep, HbA1c and Microalbuminuria for Ret were found to be most significantly associated in this Diabetes patients’ cohort. Conclusion: The proportion of microvascular complications was found to be significantly high among patients with Type II diabetes mellitus in this cohort. Controlling HbA1c could help reduce all four types of complications. However, controlling microalbuminuria could prevent DPN and Ret. This emphasizes the need for screening and prevention program toward early, symptomless identification of type II diabetes microvascular complications.
The Impact of Nipple Repair in the Treatment of Lactating Nipple Injury
Authors: GAO HAIFENG , Wang jie , Ding Songtao , Li Yan , Zhang Yi , Gao Yajun , He Xiangping , Chen Si , Liu Xingxing , Zhang Hongwei , Liu Hongyan , Chen Ying 
Published: May 2020

Abstract
Objective To explore the effect of nipple repair in the treatment of lactating nipple injury.Methods Patients with nipple lesions admitted to the Maternal and Child Health Hospital of Haidian District from December 2015 to January 2020 were retrospective control study. The patients received nipple repair was the study group, the patients did not treated with medicine or surgery was the control group. Patients in both groups continued to be exclusively breastfeed with good sucking, The method of chi-square test was adopted by using SPSS24.0 statistical software to compare the difference of healing effect of nipple injury, pain relief rate, and recurrence rate of nipple injury.Results A total of 167 patients were enrolled, including in the 81 study group and 86 in the control group. The cure rates of the nipple injury in the study group and in the control group were 54.3% (44/81) and 26.7% (23/86) (χ2 = 13.451, P = 0.001). The complete pain relief rate was 48.1% (39/81) and 23.3% (20/86) (χ2 = 14.170, P = 0.001). The recurrence rate was 36.4% (16/44) and 34.8% (8/23) (χ2 = 0.016, P = 0.898).Conclusion For patients with no improvement in nipple damage after correction of etiology, nipple repair can improve the healing environment of nipple breakage, relieve nipple pain.
Using Best-Worst Scaling to Understand the Preferences of Stroke Survivors, their Relatives/Carers and the Public on Providing Stroke Thrombectomy Services in England
Authors: Flynn Darren , Lumley Hannah , Coughlan Diarmuid , Burgess David , Robinson Tomos , Ford Gary A , McMeekin Peter , White Phil 
Published: May 2020

Abstract
Background Outcomes for acute ischaemic stroke patients with large artery occlusion are significantly improved with endovascular thrombectomy (EVT). We aimed to identify preferences of stroke survivors, their relatives/carers and the public in England on: (i) EVT service organisation (localised versus centralised expert-delivered EVT services) and (ii) modelled outcomes for EVT effectiveness, equity of access and costs of EVT provision (based on increasing the number of comprehensive stroke centres across England that provide EVT from 24 to 30).Methods Stroke survivors, their relatives/carers and the public were engaged in an iterative co-design process to develop an accessible survey incorporating two Best-Worst Scaling (BWS) tasks: EVT service organisation (4 attributes each with 2 levels) and modelled outcomes (3 attributes each with 2 levels). On-line pilot testing was undertaken with 10 stroke survivors/carers. BWS data were transformed into standardised scores with corresponding 95% confidence intervals (CIs).Results 105 respondents completed the survey (stroke survivors [18%]; relative/carer of stroke survivor [32%]; member of public [50%]). Centralised (local) EVT provision by experienced and specialised medical teams was associated with the strongest positive preference (0.76, 95% CIs = 0.69, 0.82), followed by secondary inter-hospital transfer for EVT; associated with positive preferences whether required (0.15, 95% CIs = 0.07, 0.22) or not (0.30, 95% CIs = 0.22, 0.38). Travel times by emergency ambulance > 45 min (-0.29, 95% CIs=-0.37, -0.22) were associated with stronger negative preferences than length of stay < 48 hours [-0.16, 95% CIs=- 0.22, − 0.10). Maximal effectiveness of EVT (52% recovering with none/mild disability) was associated with stronger positive preferences (0.40, 95% CIs = 0.27, 0.53) than equity of access (71% or 72% of eligible patients) to EVT within 7 hours since onset of stroke symptoms, which was associated with marginal positive preferences. Costs of EVT provision were associated with negative preferences; maximal cost had the strongest negative preference (-0.29, 95% CIs=-0.40, -0.18).Conclusions Centralised stroke centres with experienced specialised teams and secondary transfers with travel times ≤ 45 minutes are likely to be acceptable to patients/carers and the public. Equity of access to EVT may be less of a priority than effectiveness, although both were considered more important than costs of EVT provision.
Kidney Injury Molecule-1 Mediated Autophagy Pathway Participates in Ischemic Preconditioning Evoked Renal Protection
Authors: Qian Subo , Li Xinyan , Gu Zhengqin , Shen Haibo 
Published: May 2020

Abstract
Background

Ischemia-reperfusion injury (IRI) is one of the most vital pathogenesis causing kidney injury, especially during the perioperative periods of partial nephrectomy and renal transplantation, but lacking of effective prevention and treatment strategies. This study was conducted to investigate the influences of ischemic preconditioning (IPC) on the pathological process of mouse bilateral IRI, and to figure out the role of kidney injury molecule-1 (KIM-1) mediated autophagy pathway in this process.
Methods

Nephron major histocompatibility complex class II (MHC II) conditional knockout (cKO) mice (Six2-Cre+/−; MHC II flox/flox) and the age- and sex-matched littermates from the Six2-Cre−/−; MHC II flox/flox colony (Ctrl) were established to investigate these issues. A 15-minute period of IPC was performed 4 days before the 30-minute of bilateral renal vessel occlusion. Severity of renal IRI in cKO and Ctrl mice with or without IPC were analyzed respectively and correspondingly.
Results

MHC II cKO mice presented severer kidney injury in both acute and chronic phase of renal IRI. IPC could significantly attenuate ischemia/reperfusion-induced serum creatinine (sCr) and blood urea nitrogen (BUN) increasing, as well as histological KIM-1 expression. However, MHC II cKO mice undergoing IPC showed more deteriorated kidney injury when compared with Ctrl mice, with higher levels of sCr, BUN, KIM-1 expression in the acute phase, and aggravated interstitial fibrosis in the chronic phase.
Conclusions

IPC could attenuate renal IRI functionally and histologically. KIM-1 mediated autophagy pathway plays a vital role in the IPC induced renal IRI protection.
Decline in independence after three years and its association with dietary patterns and frailty factors in community-dwelling older people: an analysis by sex and age stage
Authors: Kodama Sayuri , Hoshi Tanji , Kurimori Sugako 
Published: May 2020

Abstract
Background
Support based on sex and age is required to implement longevity strategies. This study clarified the association between declining independence after three years and the dietary patterns and frailty factors of community-dwelling independent older people. We analyzed sex and age stages for people between 65 and 75 years (earlier-stage) and people aged 75 years or above (latter-stage), respectively.
Methods
In a longitudinal study of 25 Japanese prefectures from 2013 to 2016, 3,693 respondents completed baseline and follow-up questionnaires. We analyzed 2,250 participants (1,294 men), after excluding individuals younger than 65 years (n = 510), deceased (n = 35), with missing data (n = 866), or disabled (n = 32). Independence was evaluated based on Instrumental Activities of Daily Living (IADL) scores (maximum = 12). Disability was defined as scoring lower than 9. Dietary patterns were derived from a principal component analysis of 7 food groups. Frailty factors showing a significant relationship with baseline IADL scores were selected. Multivariate logistic regression analysis revealed an association between baseline factors and declining independence after three years.
Results
The average IADL score three years from baseline was 11.5 ± 1.1. There were two IADL score groups: “high independence” (69.6%) and “low independence” (30.4%). Three baseline dietary patterns were identified: “dietary diversity,” “high meat frequency,” “low fruit, vegetable, and dairy product frequency.” The multivariate-adjusted model in the earlier-stage revealed that BMI ≥ 30 (odds ratio (OR) = 0.97, 95% confidence interval (CI): 0.12-7.61) showed no significant difference from 18.5 < BMI (OR = 1). In the latter stage, subjective health and exercising three or more times a week with enjoyment were associated with a lower odds ratio. High meat frequency was related to high OR in all groups, especially latter-stage older women (OR = 1.59, 95% CI: 1.17‐2.15).
Conclusions
Effective integrated support for maintaining independence may be associated with weight management for earlier-stage men, the mental/psychological factors in subjective health and exercise enjoyment for latter-stage older people, and limited meat consumption, especially for latter-stage older women.
Is there any difference in the outcome of geriatric and non-geriatric severely injured patients? – A seven-year, retrospective, observational cohort study with matched-pair analysis
Authors: Jensen Kai Oliver , Lempert Maximilian , Sprengel Kai , Simmen Hans-Peter , Pothmann Carina , Schlögl Mathias , Bischoff-Ferrari Heike , Hierholzer Christian , Pape Hans-Christoph , Neuhaus Valentin 
Published: May 2020

Abstract
Background:
The aging population has a tremendous impact on health care. Geriatric trauma is expected to increase due to the lifestyle and activity of the aging population and will be one of the major future challenges in health care. Therefore, the aim of this study was to investigate differences between polytraumatized geriatric and non-geriatric patients regarding 1) mortality, 2) length of stay, and 3) complications with a matched pair analysis.
Methods:
We included patients older than 17 years with an Injury Severity Score (ISS) of 16 or more admitted to our level 1 trauma center between January 2008 and December 2015. The cohort was stratified into two groups (age < 70 years “non-geriatric”, age ≥ 70 years “geriatric”). One-to-one matching was performed based on gender, ISS, mechanism of injury (penetrating/blunt), Glasgow Coma Scale (GCS), base excess, and the presence of coagulopathy (INR ≥ 1.4). Outcome was compared using the paired t-test and McNemar-test.
Results:
A total of 1457 patients with a mean age of 62 (± 21) years were identified. There were 1022 male (72%) and 435 female patients. Three hundred forty six patients (24%) were older than 70 years. Matching resulted in 57 pairs. 1) Mortality (25% vs 32% respectively; p = 0.219) as well as 2) length of stay (12.5 (± 13.3) days vs 11.8 (± 11.8) days respectively, p = 0.754) were comparable between geriatric and non-geriatric polytraumatized patients. 3) The complication rate (34% vs. 56%, p = 0.031) was significantly higher in geriatric patients.
Conclusion:
Despite significantly higher complication rates amongst the geriatric trauma patients, there were no significant differences regarding mortality and length of stay in this matched pair analysis – indicating the possibility of similar outcome in geriatric polytraumatized patients receiving optimal care.