bluuebunny/crossref_metadata_2025_split huggingface dataset:
Therapeutic potential of human induced pluripotent stem cells and renal progenitor cells in experimental chronic kidney disease
Authors: Ribeiro Patrícia de Carvalho , Lojudice Fernando Henrique , Fernandes-Charpiot Ida Maria Maximina , Baptista Maria Alice Sperto Ferreira , Araújo Stanley de Almeida , Mendes Gloria Elisa Florido , Sogayar Mari Cleide , Abbud-Filho Mario , Caldas Heloisa Cristina 
Published: September 2020

Abstract
Background: Chronic Kidney Disease (CKD) is a global public health problem. Cell therapy using pluripotent stem cells represents an attractive therapeutic approach for the treatment of CKD.Methods: We transplanted Mitomycin C (MMC)-treated human induced pluripotent stem cells (hiPSCs) and renal progenitor cells (RPCs) into a CKD rat model system. The RPCs and hiPSCs cells were characterized by immunofluorescence and qRT-PCR. Untreated 5/6 nephrectomized rats were compared to CKD animals receiving the same amount of MMC-treated hiPSCs or RPCs. Renal function, histology and immunohistochemistry were evaluated 45 days post-surgery.Results: We successfully generated hiPSCs from peripheral blood and differentiated them into RPCs expressing renal progenitor genes (PAX2, WT1, SIX2, and SALL1) and podocyte-related genes (SYNPO, NPHS1). RPCs also exhibited reduced OCT4 expression, confirming the loss of pluripotency. After cell transplantation into CKD rats, the body weight change was significantly increased in both hiPSC and RPC groups, in comparison with the control group. Creatinine clearance (CCr) was preserved only in the hiPSC group. Similarly, the number of macrophages in the kidneys of the hiPSC group reached a statistically significant reduction, when compared to control rats. Both treatments reduced positive staining for the marker α-smooth muscle actin. Histological features showed decreased tubulointerstitial damage (interstitial fibrosis and tubular atrophy) as well as a reduction in glomerulosclerosis in both iPSC and RPC groups.Conclusions: In conclusion, we describe that both MMC-treated hiPSCs and RPCs exert beneficial effects in attenuating CKD progression. Both cell types were equally efficient to reduce histological damage and weight loss caused by CKD. hiPSCs seems to be more efficient than RPCs, possibly due to a paracrine effect triggered by hiPSCs. These results demonstrate that the use of MMC-treated hiPSCs and RPCs improve clinical and histological CKD parameters, avoided tumor formation, and therefore may be a promising cell therapy strategy for CKD.
Mild Cognitive Impairment in novel SPG11 Mutation-Related Sporadic Hereditary Spastic Paraplegia with Thin Corpus Callosum
Authors: Li Chuan , Yan Qi , Duan Feng-ju , Zhao Chao , Zhang Zhuo , Du Ying , Zhang Wei 
Published: September 2020

Abstract
Background: SPG11 mutation-related autosomal recessive hereditary spastic paraplegia with thin corpus callosum (HSP-TCC) is the most common cause in complicated forms of HSP, usually presenting comprehensive mental retardation on early-onset stage preceding spastic paraplegias in childhood. However, there are still lots of sporadic late-onset HSP-TCC cases with negative family history, and potential mild cognitive deficits in multiple domains may be easily neglected and inaccurately described. Methods: In this study, we performed next generation sequencing in four sporadic late-onset patients with spastic paraplegia and thin corpus callosum (TCC), and combined Mini-Mental State Examination (MMSE) and Montreal Cognitive Assessment (MoCA) to evaluate cognition of the patients. Results: By evolutionary conservation and structural modeling analysis, we have revealed 4 novel pathogenic SPG11 mutations, and firstly confirmed mild cognitive impairment (MCI) with normal MMSE scores (≥27) and decreased MoCA scores (<26) in these SPG11 mutation-related HSP-TCC patients, predominantly presenting impairment of visuoexecutive function, delayed recall, abstraction and language correlated with prefrontal deficits.Conclusions: The results expand the mutational spectrum of SPG11-associated HSP-TCC from sporadic cases, and confirm MCI characterized with dysfunction of prefrontal lobe in SPG11-related HSP-TCC, which should be paid more attention by neurologists.
Distribution Measurements of Chimeric Antigen Receptor-Modified T Cells Against CD19
Authors: Ying Zhitao , He Ting , Wang Xiaopei , Zheng Wen , Lin Ningjing , Tu Meifeng , Xie Yan , Ping Lingyan , Zhang Chen , Liu Weiping , Deng Lijuan , Wu Meng , Feng Feier , Leng Xin , Du Tingting , Qi Feifei , Hu Xuelian , Ding Yanping , Lu xinan , Song Yuqin , Zhu Jun 
Published: September 2020

Abstract
Backgroud: The unprecedented efficacy of chimeric antigen receptor (CAR) T-cell immunotherapy of CD19+ B-cell malignancies has opened a new and useful way for the treatment of malignant tumor. Nonetheless, there are still formidable challenges in the field of CAR-T cell therapy, such as the biodistribution of CAR-T cells in vivo.Methods: We demonstrated the distribution of CAR-T cells in the absence of target cells or with target cells in the mice and the dynamic changes in the patient blood over time after infusion were deteced by qPCR and FACS. Results: CAR-T cells still proliferated in the mice without target cells and peaked at 2 weeks. However, CAR-T cells did not increase significantly in the presence of target cells within 2 weeks after infusion, but expanded at 6 weeks. In the clinical trial, we found that CAR-T cells peaked at 7-21days after infusion and can last for as long as 510 days in the peripheral blood of patients. Simultaneously, mild side-effects were noted which can be effectively controlled within two months in these patients.Conclusions: CAR-T cells can expand themselves with or without target cells in mice. CAR-T cells can persistence for a long time in patients.
Evaluation of Salivary Biomarkers for the Diagnosis of Periodontitis
Authors: Zhang Yong , Kang Ni , Xue Fei , Qiao Jing , Duan Jinyu , Chen Fan , Cai Yu 
Published: September 2020

Abstract
Background: To investigate the diagnostic potential of salivary biomarkers and built a prediction panel for diagnosing periodontal disease. Methods: In a cross-sectional study, a total of 85 participants were enrolled in the study and divided into healthy, gingivitis and periodontitis groups based on their periodontal examine results, a full mouth periodontal examination were received and unstimulated saliva were collected. Salivary IL-1β, MMP-8, ICTP and Pg were assessed and their potential of diagnosing periodontal disease were analyzed, the combinational prediction panels of periodontal disease were evaluated. Results: The periodontitis group showed significantly higher level of IL-1β, MMP-8, ICTP and Pg compared to healthy group and higher level of IL-1β, ICTP and Pg compared to gingivitis group, the gingivitis group showed higher level of IL-1β, MMP-8 and Pg compared to healthy group. As a single marker, IL-1β showed the best diagnostic value of four markers and exhibited an AUC value of 0.88 with 94% sensitivity and 71% specificity to discriminate periodontitis from healthy subjects, an AUC value of 0.81 with 82% sensitivity and 76% specificity to discriminate gingivitis from healthy subjects and an AUC value of 0.67 with 69% sensitivity and 64% specificity to discriminate periodontitis from gingivitis subjects. The combination of IL-1, ICTP and Pg exhibited the highest value to discriminate periodontitis from healthy (AUC=0.94) and gingivitis (AUC=0.78) subjects, the combination of IL-1 and MMP-8 exhibited the best value to discriminate gingivitis from healthy subjects (AUC=0.85). Conclusions: Salivary IL-1β, MMP-8, ICTP and Pg showed discriminate efficiency for diagnosing periodontal disease, the combination of IL-1, ICTP and Pg can be used to discriminate periodontitis from healthy and gingivitis subjects and the combination of IL-1 and MMP-8 can be used to discriminate gingivitis from healthy subjects.
The Patient’s Denture Assessment (Thai version) is a Valid and Reliable Tool for Evaluating Complete Denture Treatment and Success.
Authors: Namano Sahaprom , Komin Orapin 
Published: September 2020

Abstract
BACKGROUND. Complete tooth losses are still being major problems which resulted in lesser quality of life especially for elderly patients. However, there are still lack of questionnaire to evaluate the treatment outcome from the patient’s aspect. The objective of this study is to evaluate the reliability and validity of the Patient’s Denture Assessment-Thai version (PDA-T), then use this questionnaire to assess patient satisfaction with complete denture treatment. METHODS. The subjects comprised 120 edentulous adult patients (49 men/71 women; average age 70 years-old) from the Prosthodontic and the Geriatric Dentistry and Special Patients Care Clinic at the Faculty of Dentistry, Chulalongkorn University during 2019 March‒2020 March. The patients were divided into two groups: The group experienced (Exper) (n=54) with wearing complete dentures, and the non-experienced (NonExper) group (n=66). The patients used the validated PDA-T to self-assess their treatment at different times. The Exper group completed the questionnaire at t 0 (during treatment), t 0.5 (2‒8-weeks after t 0 ), and t 1 (final follow-up). The NonExper group completed the questionnaire only at t 1 . RESULTS. In the Exper group, Cronbach’s α and average inter-item correlation was 0.95 (range 0.76‒0.95) and 0.47 (range 0.57‒0.83), respectively. The intraclass correlation coefficients (n = 18, 95% confidence interval) were 0.98 overall. The paired t-test (p < 0.05) between t 0 and t 1 indicated a significant difference between t 0 and t 1 in every PDA-T topic, and the effect size was 1.71. In the NonExper group, the Pearson correlation analysis indicated no significant correlation between the patients' demographics and masticatory function. CONCLUSIONS. The reliability and validity of the PDA-T indicate it is a valuable tool for evaluating complete denture treatment. Treatment success affected the patients' satisfaction, but was not associated with the patients’ prior denture experience, sex, or educational level.
The Mechanism of NF- κB/MAPKs-TLR4 Pathway in the Inhibitory Effect of Rhubarb Lingxian Formula on Inflammatory Response of Bile Duct Cells
Authors: Zhang Man , Li Minpeng , Yu Yuan , Xu Bin , Fan Fang , Wu Huashuai , Chen Jinmei 
Published: September 2020

Abstract
Background: To explore the mechanism of NF- κB/MAPKs-TLR4 signal pathway in the inhibitory effect of rhubarb lingxian formula on inflammatory response of bile duct cells.Methods: The chronic inflammatory state of bile duct in rats with primary choledocholithiasis was established by injecting LPS into common bile duct. Rat intrahepatic bile duct epithelial cells were isolated and purified. The proliferation activity of bile duct epithelial cells was detected by CCK-8. Blank group, LPS control group, LPS + NF-κB blocker (PDTC) group, LPS + p38MAPK blocker (SB203580) group, LPS + PTDC + SB203580 group, LPS + rhubarb lingxian group, LPS + PDTC + rhubarb lingxian group, LPS + SB203580 + rhubarb lingxian group and LPS + PTDC + SB203580 + rhubarb lingxian group were constructed, respectively. The NF- κB, MAPKs and TLR4 mRNA levels were detected by RT-qPCR, whereas NF-κB, MAPKs, TLR4, TNF-α and IL-6 protein levels were detected by immunohistochemical staining and Western blot.Results: The mRNA and corresponding proteins of NF-κB and MAPKs in the LPS control group were noteworthily increased in contrast to the blank group (P < 0.01), which evidently lower in the LPS + rhubarb lingxian group than those presented in the LPS control group (P < 0.01). The activity of NF- κB and MAPKs channel in the LPS + PTDC + SB203580 group was suppressed simultaneously in comparison to the LPS control group, which further caused decreased TLR4 mRNA expression. Meanwhile, the TLR4 mRNA expression in LPS + traditional Chinese medicine group also decreased (P < 0.01). IL-6 and TNF- α protein expression in bile duct epithelial cells of rats in LPS control group increased apparently than those of blank group. In contrast to LPS control group, TNF- α and IL-6 protein expressions in each treatment group decreased in different degrees (P < 0.01), which decreased at most in LPS + X (X can be blank or blocker) group or LPS+ PTDC+ SB203580 group, followed by LPS + Y (Y is a single blocker), and finally by the LPS treatment group.Conclusion: Rhubarb lingxian formula may inhibit the inflammatory reaction of bile duct epithelium in rats by down-regulating the activity of NF-κ B / MAPKs-TLR4 signal pathway.
Effect of quadriceps strengthening exercises alone and in combination with Baduanjin qigong on older adults with knee osteoarthritis: a quasi-experimental study
Authors: wang fenglan , Zhang Xiaoli , Tong Xiao , Zhang Min , Xing Fengmei , Yang Kun , Jiao Nana , Duan Zhiguang 
Published: September 2020

Abstract
BackgroundExercise is recommended as a core treatment for individuals with KOA. However, the optimal exercise program to promote long-term compliance for KOA patients is not clear. The aim of this study is to compare the effects of the combination exercise program (quadriceps strengthening exercises (QSE) plus Baduanjin qigong) versus QSE alone on older adults with knee osteoarthritis (KOA). MethodsA two-arm, quasi-experimental trial with repeated measurements was used. As a cluster randomized trial, participants from one community center were assigned to combination exercise group while participants from the other center were assigned to QSE group. We assessed pain intensity, physical function, self-efficacy, and HRQoL with standardized instruments at baseline, 3 and 6 months. Results87 participants with KOA who aged above 60 completed the study. Over the 6 months, There were significant time by group interaction effects on pain intensity (F = 44.419, p < 0.001), physical function (F = 46.904, p < 0.001) and self-efficacy (F = 49.754, p < 0.001), as well as in the physical component summary (F = 15.205, p < 0.001) and mental component summary of SF-12 (F = 19.205, p < 0.001), with the combination exercise group exhibiting significantly greater improvements in all outcomes than QSE group. ConclusionsCombination exercise treatment is more effective than QSE for relieving pain, increasing physical functioning, and improving self-efficacy, and quality of life in community-dwelling KOA older adults. Also, it could promote long-term compliance for KOA community patients.Trial registrationChinese Clinical Trails Registry number ChiCTR2000033387 (retrospectively registered). Registered 30 May 2020
Identification and Validation of a Five-lncRNA Signature Related to Glioma Using Bioinformatics Analysis
Authors: Tian Daofeng , Liu Haitao , Xu Pengfei , Ye Liguo , Wang Long , Tan Yinqiu , Liu Baohui , Chen Qianxue 
Published: September 2020

Abstract
BackgroundTo accurately predict the prognosis of glioma patients. Methods and ResultsA total of 541 samples from the TCGA cohort and 181 observations from the CGGA database were included in our study. By weighted gene co-expression network analysis (WGCNA), 14 long non-coding RNAs (lncRNAs) associated with glioma grade were identified. Using univariate and multivariate Cox analysis Five lncRNAs (CYTOR, MIR155HG, LINC00641, AC120036.4 and PWAR6) were selected to develop the prognostic signature. The Kaplan-Meier curve depicted that the patients in high risk group had poor prognosis in both cohorts. The areas under the receiver operating characteristic curve of the signature in predicting the survival of glioma patients at 1, 3, and 5 years were 0.84, 0.92, and 0.90 in the CGGA cohort and 0.8, 0.85 and 0.77 in the TCGA set. Multivariate Cox analysis demonstrated that the five-lncRNA signature was an independent prognostic indicator in both sets (HR = 2.002, p < 0.001; HR = 1.243, p = 0.007, respectively). A nomogram including the lncRNAs signature and clinical covariates was constructed and demonstrated high predictive accuracy in predicting 1-, 3- and 5-year survival probability of glioma patients. Conclusion﻿We established a five-lncRNA signature as a potentially reliable tool for survival prediction of glioma patients.
Fraction exhaled nitric oxide and IOS as potential biomarkers for HDM immunotherapy in patients with allergic rhinitis and asthma
Authors: Zhou Jun , Du Wei , Xu Guofang , Wang Ping , Tao Yi , Sun Lin , Peng Bo , Dai Ranran , Tang Wei 
Published: September 2020

Abstract
BackgroundAllergen specific immunotherapy (AIT) is the only potential disease-modifying treatment of house dust mite (HDM) allergic asthma and rhinitis. The conventional treatment procedure lasted for 3 years, but biomarkers indicating early efficacy from this long-term treatment has not been well illustrated. In this single arm, observation study, we evaluated non-invasive airway inflammation parameters such as Exhaled nitric oxide (FeNO), nasal NO levels (FnNO), and small airway function parameter-impulse oscillometry system (IOS) as well as serum parameters Dermatophagoides pteronyssinus (Derp) specific IgG4 and sIgG4/IgE ratio as potential biomarkers for AIT.MethodsPatients with mild allergic asthma concomitant with allergic rhinitis were treated with subcutaneous immunotherapy of Der p extract. Clinical evaluations including symptom score and medication score, small airway function of spirometer and non-invasive airway inflammation parameters from the nose (FnNO) and lung (FeNO) were collected before and after half year and one year of AIT. Serum Derp specific IgE and IgG4 measurement data were also collected. ResultsSymptoms and medication score of patients were alleviated both after half and one-year HDM immunotherapy according to VAS (visual analogue score) and medication score. Improved small airway function (evaluated through IOS) and reduced non-invasive airway allergic inflammation parameters both from the nose (FnNO) and lung (FeNO) were achieved after half and one year AIT. As to serum biomarkers, Der p specific IgG4 increased and Der P specific IgE/IgG4 decreased significantly after one year treatment.ConclusionsOne year HDM subcutaneous allergen immunotherapy improved allergic symptoms and reduced medication score in patients with allergic asthma concomitant with rhinitis. Non-invasive airway inflammation parameters such as FeNO and FnNO and small airway function parameter IOS are potential biomarkers for evaluating early efficacy of AIT. Derp specific IgG4 as well as IgE/IgG4 ratio maybe good therapeutic predictors to reflect the efficacy of AIT in the first year of treatment.
Evaluation of Small Hydropower Plant at Ribb Irrigation Dam in Amhara Regional State, Ethiopia
Authors: BEZABIH ADDISU WORKU 
Published: September 2020

Abstract
Background: Energy is the main requirement for economic growth in any country and also supports the modern economy. The energy sector is considered a vital element in developing countries because it meets energy needs. This article deals with the production of electricity using small hydroelectric power plants for rural applications. The main objective of this study was to assess the potential of the Ribb dam for small hydroelectric plants. Since the height of a proposed site is constant while the available flow is very variable, the flow is very important for the production of hydroelectricity. Result: The flow duration curve is constant from 20% to 50% of the nominal flow, and then decreases as the percentage of the nominal flow increases. The Ribb dam concluded that the average flow of 14.6331m3/s, the smooth head of 70.37 m, and the average nominal power of 5.53 MW would be the most optimal size of a small hydroelectric plant producing a maximum of electricity in the context of future projected flows in the study region. Conclusion: SHP is a promising alternative for the production of cheap and renewable energy in rural or developing areas.
Inoculation of Native Symbiotic Effective Sinorhizobium Spp. Enhanced Soybean [Glycine Max (L) Merr.]&nbsp;Grain Yield in Ethiopia
Authors: Dagaga Diriba Temesgen , Tuji Fassil Assefa 
Published: September 2020

Abstract
BackgroundSoybean [Glycine max (L) Merr.] is an annual leguminous crop serving as a source of food and feed, green manure, biodiesel and fiber It is nodulated by diverse slow growing and fast growing rhizobia belonging to the genus Bradyrhizobium and Sinorhizobium, respectively. In Ethiopia, it has been cultivated since 1950s with lower grain yield history. Yield improvement efforts have been more concentrated on agronomic studies, inoculation of exotic Bradyrhizobium japonicum including TAL379 and/or fertilizer application. The results have usually been unsatisfactory and inconsistent. This study was initiated to identify promising indigenous soybean rhizobial inoculant that can enhance yield of the crop in the country.Methods Native soybean rhizobia, designated GMR for Glycine max rhizobia, were trapped using soybean (cv. Ethio-Yugoslavia) from soils collected across agro-ecologies of Ethiopia. Indigenous soybean rhizobia were screened for in vitro tolerance against physico-chemical stresses, plant growth promoting (PGP) traits and symbiotic performances at greenhouse and field levels A reference B. japonicum (TAL379) was included in all experiments. A soybean plant growth promoting Achromobacter sp. was also included in field trials for co-inoculation. Quantitative data were assessed by analysis of variance (ANOVA) employing SAS computer software package version 9.3. Mean separations were undertaken using the Duncan’s Multiple Range Test at p≤0.05.Result GMR that produced acid and grew faster with larger colonies were identified as Sinorhizobium spp. and those which produced alkali and grew slowly with smaller colonies were identified as Bradyrhizobium spp though further genetic analysis should be performed for verification and identification of their genus and species, respectively. Two Sinorhizobium spp (GMR120C and GMR125B) profoundly nodulated different soybean cultivars under greenhouse conditions and significantly improved grain yield (p≤0.05; maximum 3.98 tons ha-1) compared to 2.41, 2.82 and 2.69 recorded as maximum grain yield (tons ha-1) for TAL379 inoculation, positive control and negative control, respectively in field trials. Higher yield was recorded when GMR125B was coinoculated with Achromobacter sp., but when GMR120C was inoculated singly. These GMR also showed efficient utilization of numerous substrates, some PGP traits and potential adaptation to various ecological stresses. Conclusion The two Sinorhizobium spp. (GMR120C and GMR125B) are promising soybean inoculants that can be used to enhance the productivity of the crop in the country.