bluuebunny/crossref_metadata_2025_split huggingface dataset:
The E2F family as potential biomarkers and therapeutic targets in colon cancer
Authors: Yao Haibo , Lu Fang , Shao Yanfei 
Published: February 2020

Background
The E2F family is a group of genes encoding a series of transcription factors in higher eukaryotes and participating in the regulation of cell cycle and DNA synthesis in mammals. This study was designed to investigate the role of E2F family in colon cancer.

Methods
In this study, the transcriptional levels of E2F1-8 in patients with colon cancer from GEPIA was examined. Meanwhile, the immunohistochemical data of the eight genes were also obtained in the The Human Protein Atlas website. Additionally, we re-identified the mRNA expression levels of these genes via real time PCR. Furthermore, the association between the levels of E2F family and stage plot as wells overall survival of patients with colon cancer were analyzed.

Results
We found that the mRNA and protein levels of E2F1, E2F2, E3F3, E2F5, E2F7 and E2F8 were significantly higher in colon cancer tissues than in normal colon tissues while the expression levels of E2F4 and E2F6 displayed no significant difference between colon cancer tissues and normal tissues. Additionally, E2F3, E2F4, E2F7 and E2F8 were significantly associated with the stages of colon cancer. The Kaplan-Meier Plotter showed that the high levels of E2F3 conferred a worse overall survival and disease free survival of patients with colon cancer. Also, high levels of E2F4 resulted in a worse overall survival.

Conclusion
Our study implied that E2F3, E2F4, E2F7 and E2F8 are potential targets of precision therapy for patients with colon cancer while E2F1, E2F2, E3F3, E2F5, E2F7 and E2F8 are potential biomarkers for the diagnosis of colon cancer.
Care Strategies for the Elderly with Alzheimer's Disease: Integrative Literature Review
Authors: Aguiar Viviane Ferraz Ferreira de , Carvalho Dayara de Nazaré Rosa de , Sardinha Daniele Melo , Martins Jaqueline Dantas Neres , Costa Rafael Everton Assunção Ribeiro da , Cunha Carla Sena , Sobrinho Celice Ruanda Oliveira , Trindade Fernanda Araújo , Silva Rakel Daiane Costa da , Sousa Ivone de Melo , Bendelaque Dandara de Fátima Ribeiro , Castro Kateley Stefany da Costa , Oliveira Andréia Dias Grijó de , Galvão Raphael Resende Gustavo , Neves Lorena Nayara Alves , Nazaré Nancy de Souza Felipe de , Souza Suzanne Lourdes , Pamplona Mônica Custódio do Couto Abreu , Feitosa Elisa da Silva , Peixoto Ivonete Vieira Pereira 
Published: February 2020

Objective: To investigate care strategies in older people with Alzheimer's Disease, in the 2014-2018 historical series.
Methodology: Integrative Literature Review was conducted in the Scientific Electronic Library Online (SciELO), Latin American Health Sciences Literature (LILACS) and Nursing Databases (BDENF) databases.
Results: 10 articles were found and analyzed using IRAMUTEC software. This recognized the separation of the corpus into 10 initial context units (ICU), with the emergence of the following categories: 1) Family dynamics and care; 2) Nursing care for the elderly with Alzheimer's disease; 3) Geronto-technologies and their use for the elderly with Alzheimer's disease.
Conclusion: The disease interferes in the ability to meet the basic needs of the elderly, thus the need for specific care arises, and it is essential that the nurse outlines his therapeutic plan also to the caregiver and the elderly.
Hijama Practices and the Perceived Health Benefits among the People of Al-Qassim Region, Saudi Arabia
Authors: Al-luhaidan Khawla , Prarthana M. S. 
Published: February 2020

Introduction: Hijama (wet cupping therapy) is a form of traditional alternative medicine that has been in practice for thousands of years and was adopted by many different cultures. Hijama practice in the Arabic medical literature has been reported to treat many diseases differing in etiology and pathogenesis. The aim objective of this study was to determine the knowledge, attitude, and practice of Hijama among the Qassim population in Saudi Arabia. To create awareness regarding good practices and to assess the effectiveness of Hijama.
Methods: This observational type of cross-sectional survey included 201 participants who were all practising Hijama and were from the Qassim region. Data was collected using the self-administered online questionnaire and entered in Excel and analyzed using EpiInfo7 statistical software.
Results: Most of the study participants were female 70%. About 39% of them have a bachelor's degree. 61% know Hijama contradictions. Hijama was performed by traditional healers in 14% of the participants and at Hijama centers by 64% of the participants. About 72% of the participants notice effectiveness by practising Hijama. Only 7% of them had suffered complications.
Conclusion: The study revealed Hijama can be a simple effective economic alternative treatment that can be used to treat many ailments like back, shoulder, neck pain, headache and migraine pain. It is associated with a minimal side effect. There is still a need to create awareness among the people to go for clinical centres to perform Hijama. Future research is needed to support its therapeutic benefits.
Tuning Neuronal Circuit Formation in 3D Polymeric Scaffolds by Introducing Graphene at the Bio/Material Interface
Authors: Rauti Rossana , Secomandi Nicola , Martín Cristina , Bosi Susanna , Severino Francesco P. U. , Scaini Denis , Prato Maurizio , Vázquez Ester , Ballerini Laura 
Published: February 2020

Abstract2D cultures are useful platforms allowing studies of the fundamental mechanisms governing neuron and synapse functions. Yet, such models are limited when exploring changes in network dynamics due to 3D‐space topologies. 3D platforms fill this gap and favor investigating topologies closer to the real brain organization. Graphene, an atom‐thick layer of carbon, possesses remarkable properties and since its discovery is considered a highly promising material in neuroscience developments. Here, elastomeric 3D platforms endowed with graphene cues are exploited to modulate neuronal circuits when interfaced to graphene in 3D topology. Ex vivo neuronal networks are successfully reconstructed within 3D scaffolds, with and without graphene, characterized by comparable size and morphology. By confocal microscopy and live imaging, the 3D architecture of synaptic networks is documented to sustain a high rate of bursting in 3D scaffolds, an activity further increased by graphene interfacing. Changes are reported in the excitation/inhibition ratio, potentially following 3D‐graphene interfacing. A hypothesis is thus proposed, where the combination of synapse formation under 3D architecture and graphene interfaces affects the maturation of GABAergic inhibition. This will tune the balance between hyperpolarizing and depolarizing responses, potentially contributing to network synchronization in the absence of changes in GABAergic phenotype expression.
Functional Biomaterials for Bone Regeneration: A Lesson in Complex Biology
Authors: Armiento Angela Rita , Hatt Luan Phelipe , Sanchez Rosenberg Guillermo , Thompson Keith , Stoddart Martin James 
Published: February 2020

AbstractBone is a hard yet dynamic tissue with remarkable healing capacities. Research to date has greatly advanced the understanding of how bone heals and has led to marked success in the treatment of bone injuries. Nevertheless, the effective treatment of nonunions and large bone defects continues to present a challenge for orthopedic surgeons. Biomaterials provide researchers with a powerful instrument to potentially guide effective bone tissue regeneration in challenging healing environments. However, the most appropriate biomaterial for bone tissue engineering continues to be an area of intense debate. Indeed, the mechanical properties of real bone can be reproduced in vitro but the development of a functional bone substitute goes beyond the sole mechanical properties. The faithful reproduction of bone as a functional organ requires the combination of different cell types and temporal regulation of the molecular signaling involved during the different stages of bone formation and regeneration. This is not at all a trivial task. Herein, critical aspects of bone healing/regeneration including mechanical loading, inflammation, vascularization, and innervation are described. The success and the challenges behind the development of biomaterials, and the technologies used to functionalize them, in order to support the underlying cellular mechanisms are also highlighted.
Solution‐Processed High‐Quality Cesium Lead Bromine Perovskite Photodetectors with High Detectivity for Application in Visible Light Communication
Authors: Liu Ronghuan , Zhang Jianqiang , Zhou Hai , Song Zehao , Song Zhaoning , Grice Corey R. , Wu Dingjun , Shen Liangping , Wang Hao 
Published: February 2020

AbstractCesium lead bromide (CsPbBr3) perovskite photodetectors (PDs) are attractive for applications in visible light communication (VLC) due to ultra‐high detectivity and fast response speed. However, the fabrication of high‐quality CsPbBr3 polycrystalline films using solution‐based process is very challenging. Due to the low solubility of CsBr in precursor solutions, solution‐processed CsPbBr3 films are typically discontinuous and porous, hindering the performance of resulting PDs. Herein, a facile and modified sequential spin‐coating method is introduced to prepare high‐crystallinity, pinhole‐free CsPb2Br5‐CsPbBr3 perovskite films with an average grain size of ≈1 µm. The hole‐transport‐layer‐free (HTL‐free) PDs based on the CsPb2Br5‐CsPbBr3 perovskite films show high performance parameters, including the responsivity of 0.11 A W−1, the detectivity of 1.4 × 1012 Jones, a linear dynamic range of 128.6, and an on/off ratio of 1.5 × 106. The PDs outperform other HTL‐free perovskite PDs and are comparable to the p–i–n perovskite PDs reported in the literature. In addition, the high‐performance CsPb2Br5‐CsPbBr3 PDs are applied in VLC by using the PD as a self‐powered signal receiver of voice commands in a simulated room. This work uniquely combines the features of high‐performance self‐powered perovskite PDs with VLC techniques, paving the path to wide applications of all‐inorganic perovskite PDs.
Immunoglobulin G4‐related sclerosing cholangitis in a 3 years of age boy
Authors: Hsu Chien‐Ting , Jeng Yung‐Ming , Wu Jia‐Feng 
Published: February 2020

AbstractImmunoglobulin G4‐related sclerosing cholangitis (IgG4‐SC) is the biliary manifestation of IgG4‐related disease (IgG4‐RD). IgG4‐RD mostly has been described in adults; hence, it is a relatively new disease and generally unknown to pediatricians. There are few reported cases about pediatric IgG4‐RD. This report describes a 3 years of age boy who presented with abnormal liver function test, irregular dilatation of hepatic duct, and intermittent blood in stool which are initially diagnosed with autoimmune sclerosing cholangitis and ulcerative colitis. During follow‐up, the patient was found to have an elevated IgG4 level. The liver biopsy showed numerous IgG4 positive plasmocytic cells infiltrating (>10/high power field). It fulfills the criteria of IgG4‐SC. He was finally diagnosed as IgG4‐SC. Within 2 years treatment, he had good response with immunosuppressant therapy.
Variation in morphological traits affects dispersal and seedling emergence in dispersive diaspores of Geropogon hybridus
Authors: Chen Si‐Chong , Giladi Itamar 
Published: February 2020

PremiseIntraspecific variation in diaspore characteristics could affect various aspects of plant performance at the population, individual plant, and seed levels. We quantified variation in dispersal traits in a wind‐dispersed annual, Geropogon hybridus (Asteraceae), focusing on continuous morphological traits of dispersive diaspores and their relationships to dispersal ability and seedling emergence.MethodsWe measured the morphological traits, terminal velocity, and seedling emergence of 1140 seeds from 10 populations in two successive years. We assessed the variation in traits among three hierarchical levels of organization and between years, and quantified their effects on diaspore terminal velocity and seedling emergence.ResultsDiaspore morphological traits varied substantially at the population, plant, and diaspore levels. Variables of pappus geometry, especially pappus width and pappus opening angle, were consistent between years and were found to be the best predictors of diaspore terminal velocity and seedling emergence. There was a significant negative relationship between diaspore terminal velocity and seedling emergence.ConclusionsThe intraspecific variation in diaspore traits is sufficiently large to substantially allow a dispersal–dormancy trade‐off of individual diaspores. Our results support the hypothesis that traits of dispersive diaspores evolve in concert to select for increased dispersal potential, and provide an avenue to predict plant offspring performance through simply measured traits.
A multicenter retrospective study of 223 patients with t(14;16) in multiple myeloma
Authors: Goldman‐Mazur Sarah , Jurczyszyn Artur , Castillo Jorge J. , Waszczuk‐Gajda Anna , Grząśko Norbert , Radocha Jakub , Bittrich Max , Kortüm K. Martin , Gozzetti Alessandro , Usnarska‐Zubkiewicz Lidia , Davila Valls Julio , Jayabalan David S. , Niesvizky Ruben , Kelman Julia , Coriu Daniel , Rosiñol Laura , Szukalski Łukasz , González‐Calle Veronica , Mateos Maria V. , Jamroziak Krzysztof , Hus Iwona , Avivi Irit , Cohen Yael , Suska Anna , Chappell Aimee , Madduri Deepu , Chhabra Saurabh , Kleman Ariel , Hari Parameswaran , Delforge Michel , Robak Paweł , Gentile Massimo , Kozłowska Izabela , Goldberg Stuart L. , Czepiel Jacek , Silbermann Rebecca , Olszewski Adam J. , Barth Peter , Mikala Gabor , Chim Chor S. , Długosz‐Danecka Monika , Grosicki Sebastian , Vesole David H. 
Published: February 2020

AbstractThe t(14;16) translocation, found in 3%‐5% of newly diagnosed (ND) multiple myeloma (MM), has been associated with adverse outcomes. However, the studies establishing the characteristics of t(14;16) included solely small cohorts. The goal of the current international, multicenter (n = 25 centers), retrospective study was to describe the characteristics and outcomes of t(14;16) patients in a large, real‐world cohort (n = 223). A substantial fraction of patients had renal impairment (24%) and hemoglobin <10 g/dL (56%) on initial presentation. Combined therapy of both immunomodulatory drug and proteasome inhibitor (PI) in the first line was used in 35% of patients. Autologous stem cell transplantation was performed in 42% of patients. With a median follow up of 4.1 years (95% CI 3.7‐18.7), the median progression‐free survival (PFS) and overall survival (OS) from first line therapy were 2.1 years (95% CI 1.5‐2.4) and 4.1 years (95% CI 3.3‐5.5), respectively. Worse OS was predicted by age > 60 years (HR = 1.65, 95% CI [1.05‐2.58]), as well as revised International Scoring System (R‐ISS) 3 (vs R‐ISS 2; HR = 2.59, 95% CI [1.59‐4.24]). In conclusion, based on the largest reported cohort of t(14;16) patients, quarter of this subset of MM patients initially presents with renal failure, while older age and the R‐ISS 3 predict poor survival.
A female with X‐linked Nephrogenic diabetes insipidus in a family with inherited central diabetes Insipidus: Case report and review of the literature
Authors: Ding Can , Beetz Rolf , Rittner Gabriele , Bartsch Oliver 
Published: February 2020

AbstractThere are two forms of diabetes insipidus, central (neurohypophyseal), and nephrogenic, caused by pathogenic variants in the AVP gene and the AVPR2 or AQP2 genes, respectively. We report on a four‐generation family, seven individuals had central diabetes insipidus (CDI) and the female index patient seen from age 16 to 26 years had (mild) nephrogenic diabetes insipidus. In her father with CDI, a known pathogenic heterozygous AVP variant c.232_234del p.(Glu78del) was identified, confirming the diagnosis of CDI in him and the other affected family members. In the proband, molecular analysis disclosed a novel heterozygous AVPR2 gene variant, c.962A > T p.(Asn321Ile) and an extremely skewed X‐inactivation, confirming X‐linked nephrogenic diabetes insipidus (XL‐NDI). Whole exome sequencing showed no further causative mutation. This is the first report on the co‐existence of CDI and NDI in one family. Our review of symptomatic female AVPR2 heterozygotes includes 23 families with at least one affected female (including this study). There were 21 different causative mutations. Mutation types in females did not differ from those in males. Both severe XL‐NDI and mild forms were reported in females. All six females with severe XL‐NDI had complete loss‐of‐function (null) mutations. The remaining 17 female probands had milder XL‐NDI caused by 14 missense variants and three null variants of the AVPR2 gene. X‐inactivation was studied in nine of these females; all showed extreme or slight skewing. The review underlines that XL‐NDI in female AVPR2 heterozygotes is always accompanied by skewed X‐inactivation, emphasizing a need for X‐inactivation studies in these females.
On the Binding of Congo Red to Amyloid Fibrils
Authors: Espargaró Alba , Llabrés Salomé , Saupe Sven J. , Curutchet Carles , Luque F. Javier , Sabaté Raimon 
Published: February 2020

AbstractAmyloids are characterized by their capacity to bind Congo red (CR), one of the most used amyloid‐specific dyes. The structural features of CR binding were unknown for years, mainly because of the lack of amyloid structures solved at high resolution. In the last few years, solid‐state NMR spectroscopy enabled the determination of the structural features of amyloids, such as the HET‐s prion forming domain (HET‐s PFD), which also has recently been used to determine the amyloid–CR interface at atomic resolution. Herein, we combine spectroscopic data with molecular docking, molecular dynamics, and excitonic quantum/molecular mechanics calculations to examine and rationalize CR binding to amyloids. In contrast to a previous assumption on the binding mode, our results suggest that CR binding to the HET‐s PFD involves a cooperative process entailing the formation of a complex with 1:1 stoichiometry. This provides a molecular basis to explain the bathochromic shift in the maximal absorbance wavelength when CR is bound to amyloids.
Improvement of Intracellular Traffic System by Overexpression of KDEL Receptor 1 in Antibody‐Producing CHO Cells
Authors: Samy Andrew , Kaneyoshi Kohei , Omasa Takeshi 
Published: February 2020

AbstractThe localization of soluble endoplasmic reticulum (ER) chaperones in the cell organelle is mediated by the C‐terminal KDEL (lysine, aspartic acid, glutamic acid and leucine) motif. This motif is recognized by the KDEL receptor, a seven‐transmembrane protein that cycles between the ER and cis‐Golgi to capture missorted KDEL chaperones from post‐ER compartments in a pH‐dependent manner. The KDEL receptor's target chaperones have a substantial role in protein folding and assembly. In this study, the gene expression level of KDEL receptor 1 shows a moderate upregulation during either ER stress or growth of Chinese hamster ovary (CHO) cells in batch culture, while the ER chaperones show higher upregulation. This might indicate the possibility of saturation of the ER retention machinery or at least hindered retention during late stage batch culture in recombinant CHO cells. KDELR1 is overexpressed in a monoclonal antibody‐producing CHO cell line to improve the intracellular chaperone retention rate in the ER. An increase in the specific productivity of IgG1 by 13.2% during the exponential phase, and 23.8% in the deceleration phase of batch culture is observed. This is the first study to focus on the ER retention system as a cell engineering target for enhancing recombinant protein production.
Microstructural densification and alignment by aspiration‐ejection influence cancer cell interactions with three‐dimensional collagen networks
Authors: Huynh Ruby N. , Yousof Manal , Ly Khanh L. , Gombedza Farai C. , Luo Xiaolong , Bandyopadhyay Bidhan C. , Raub Christopher B. 
Published: February 2020

AbstractExtracellular matrix microstructure and mechanics are crucial to breast cancer progression and invasion into surrounding tissues. The peritumor collagen network is often dense and aligned, features which in vitro models lack. Aspiration of collagen hydrogels led to densification and alignment of microstructure surrounding embedded cancer cells. Two metastasis‐derived breast cancer cell lines, MDA‐MB‐231 and MCF‐7, were cultured in initially 4 mg/ml collagen gels for 3 days after aspiration, as well as in unaspirated control hydrogels. Videomicroscopy during aspiration, and at 0, 1, and 3 days after aspiration, epifluorescence microscopy of phalloidin‐stained F‐actin cytoskeleton, histological sections, and soluble metabolic byproducts from constructs were collected to characterize effects on the embedded cell morphology, the collagen network microstructure, and proliferation. Breast cancer cells remained viable after aspiration‐ejection, proliferating slightly less than in unaspirated gels. Furthermore, MDA‐MB‐231 cells appear to partially relax the collagen network and lose alignment 3 days after aspiration. Aspiration‐ejection generated aligned, compact collagen network microstructure with immediate cell co‐orientation and higher cell number density apparently through purely physical means, though cell‐collagen contact guidance and network remodeling influence cell organization and collagen network microstructure during subsequent culture. This study establishes a platform to determine the effects of collagen density and alignment on cancer cell behavior, with translational potential for anticancer drug screening in a biomimetic three‐dimensional matrix microenvironment, or implantation in preclinical models.
Get real: Orbitofrontal cortex mediates the ability to sense reality in early adolescents
Authors: Liverani Maria Chiara , Freitas Lorena G. A. , Siffredi Vanessa , Mikneviciute Greta , Martuzzi Roberto , Meskaldij Djalel‐Eddine , Borradori Tolsa Cristina , Ha‐Vinh Leuchter Russia , Schnider Armin , Van De Ville Dimitri , Hüppi Petra Susan 
Published: February 2020

AbstractIntroductionOrbitofrontal reality filtering (ORFi) is a memory mechanism that distinguishes whether a thought is relevant to present reality or not. In adults, it is mediated by the orbitofrontal cortex (OFC). This region is still not fully developed in preteenagers, but ORFi is already active from age 7. Here, we probe the neural correlates of ORFi in early adolescents, hypothesizing that OFC mediates the sense of reality in this population.MethodsFunctional magnetic resonance images (fMRI) were acquired in 22 early adolescents during a task composed of two runs: run 1 measuring recognition capacity; run 2 measuring ORFi; each containing two types of images (conditions): distractors (D: images seen for the first time in the current run) and targets (T: images seen for the second time in the current run). Group region of interest (ROI) analysis was performed in a flexible factorial design with two factors (run and condition) using SPM12.ResultsWe found significant main effects for the experimental run and condition. The bilateral OFC activation was higher during ORFi than during the first run. Additionally, the OFC was more active while processing distractors than targets.ConclusionThese results confirm, for the first time, the role of OFC in reality filtering in early adolescents.