bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Genome-wide Library of MADM Mice for Single-Cell Genetic Mosaic Analysis
Authors: Contreras Ximena , Davaatseren Amarbayasgalan , Amberg Nicole , Hansen Andi H. , Sonntag Johanna , Andersen Lill , Bernthaler Tina , Heger Anna , Johnson Randy , Schwarz Lindsay A. , Luo Liqun , Rülicke Thomas , Hippenmeyer Simon 
Published: June 2020

SUMMARYMosaic Analysis with Double Markers (MADM) offers a unique approach to visualize and concomitantly manipulate genetically-defined cells in mice with single-cell resolution. MADM applications include the analysis of lineage; single-cell morphology and physiology; genomic imprinting phenotypes; and dissection of cell-autonomous gene functionsin vivoin health and disease. Yet, MADM could only be applied to <25% of all mouse genes on select chromosomes thus far. To overcome this limitation, we generated transgenic mice with knocked-in MADM cassettes near the centromeres of all 19 autosomes and validated their use across organs. With this resource, >96% of the entire mouse genome can now be subjected to single-cell genetic mosaic analysis. Beyond proof-of-principle, we applied our MADM library to systematically trace sister chromatid segregation in distinct mitotic cell lineages. We found striking chromosome-specific biases in segregation patterns, reflecting a putative mechanism for the asymmetric segregation of genetic determinants in somatic stem cell division.
Properties of Glial Cell at the Neuromuscular Junction are Incompatible with synaptic repair in the SOD1G37RALS mouse model
Authors: Éric Martineau , Arbour Danielle , Vallée Joanne , Richard Robitaille 
Published: June 2020

ABSTRACTAmyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease affecting motoneurons in a motor-unit (MU) dependent manner. Glial dysfunction contributes to numerous aspects of the disease. At the neuromuscular junction (NMJ), early alterations in perisynaptic Schwann cell (PSC), glial cells at this synapse, may impact their ability to regulate NMJ stability and repair. Indeed, muscarinic receptors (mAChR) regulate the repair phenotype of PSCs and are overactivated at disease-resistant NMJs (Soleusmuscle) in SOD1G37Rmice. However, it remains unknown whether this is the case at disease-vulnerable NMJs and whether it translates into an impairment of PSC-dependent repair mechanisms. We usedSoleusandSternomastoidmuscles from SOD1G37Rmice and performed Ca2+-imaging to monitor PSC activity and used immunohistochemistry to analyze their repair and phagocytic properties. We show that PSC mAChR-dependent activity was transiently increased at disease-vulnerable NMJs (Sternomastoidmuscle). Furthermore, PSCs from both muscles extended disorganized processes from denervated NMJs and failed to initiate or guide nerve terminal sprouts at disease-vulnerable NMJs, a phenomenon essential for compensatory reinnervation. This was accompanied by a failure of numerous PSCs to upregulate Galectin-3 (MAC-2), a marker of glial axonal debris phagocytosis, upon NMJ denervation in SOD1 mice. Finally, differences in these PSC-dependent NMJ repair mechanisms were MU-type dependent, thus reflecting MU vulnerability in ALS. Together, these results reveal that neuron-glia communication is ubiquitously altered at the NMJ in ALS. This appears to prevent PSCs from adopting a repair phenotype, resulting in a maladapted response to denervation at the NMJ in ALS.SIGNIFICANCE STATEMENTUnderstanding how the complex interplay between neurons and glial cells ultimately lead to the degeneration of motor neurons and loss of motor function is a fundamental question to comprehend amyotrophic lateral sclerosis. An early and persistent alteration of glial cell activity takes place at the neuromuscular junction (NMJ), the output of motor neurons, but its impact on NMJ repair remains unknown. Here, we reveal that glial cells at disease-vulnerable NMJs often fail to guide compensatory nerve terminal sprouts and to adopt a phagocytic phenotype on denervated NMJs in SOD1G37Rmice. These results show that glial cells at the NMJ elaborate an inappropriate response to NMJ degeneration in a manner that reflects motor-unit vulnerability and potentially impairs compensatory reinnervation.
Cyclin B3 activates the Anaphase-Promoting Complex/Cyclosome in meiosis and mitosis
Authors: Garrido Damien , Bourouh Mohammed , Bonneil Éric , Thibault Pierre , Swan Andrew , Archambault Vincent 
Published: June 2020

ABSTRACTIn mitosis and meiosis, chromosome segregation is triggered by the Anaphase-Promoting Complex/Cyclosome (APC/C), a multi-subunit ubiquitin ligase that targets proteins for degradation, leading to the separation of chromatids. APC/C activation requires phosphorylation of its APC3 and APC1 subunits, which allows the APC/C to bind its Cdc20 co-activator. The identity of the kinase(s) responsible for APC/C activation in vivo is unclear. Cyclin B3 is required for meiotic anaphase in flies, worms and vertebrates, but whether it activates the APC/C is unclear. We found thatDrosophilaCyclin B3 (CycB3) collaborates with PP2A-B55/Tws in embryonic development, indicating that CycB3 also promotes anaphase in mitosis. Moreover, CycB3 promotes APC/C activity and anaphase in cells in culture. We show that CycB3 physically associates with the APC/C, is required for phosphorylation of APC3, and promotes APC/C association with its co-activators. We propose that CycB3-Cdk1 directly phosphorylates the APC/C to activate it in both meiosis and mitosis.
SARS-CoV-2 infection leads to acute infection with dynamic cellular and inflammatory flux in the lung that varies across nonhuman primate species
Authors: Singh Dhiraj Kumar , Ganatra Shashank R. , Singh Bindu , Cole Journey , Alfson Kendra J. , Clemmons Elizabeth , Gazi Michal , Gonzalez Olga , Escobedo Ruby , Lee Tae-Hyung , Chatterjee Ayan , Goez-Gazi Yenny , Sharan Riti , Thippeshappa Rajesh , Gough Maya , Alvarez Cynthia , Blakley Alyssa , Ferdin Justin , Bartley Carmen , Staples Hilary , Parodi Laura , Callery Jessica , Mannino Amanda , Klaffke Benjamin , Escareno Priscilla , Platt Roy N. , Hodara Vida , Scordo Julia , Oyejide Adelekan , Ajithdoss Dharani K. , Copin Richard , Baum Alina , Kyratsous Christos , Alvarez Xavier , Rosas Bruce , Ahmed Mushtaq , Goodroe Anna , Dutton John , Hall-Ursone Shannan , Frost Patrice A. , Voges Andra K. , Ross Corinna N. , Sayers Ken , Chen Christopher , Hallam Cory , Khader Shabaana A. , Mitreva Makedonka , Anderson Timothy J. C. , Martinez-Sobrido Luis , Patterson Jean L. , Turner Joanne , Torrelles Jordi B. , Dick Edward J. , Brasky Kathleen , Schlesinger Larry S. , Giavedoni Luis D. , Carrion Ricardo , Kaushal Deepak 
Published: June 2020

SummaryThere are no known cures or vaccines for COVID-19, the defining pandemic of this era. Animal models are essential to fast track new interventions and nonhuman primate (NHP) models of other infectious diseases have proven extremely valuable. Here we compare SARS-CoV-2 infection in three species of experimentally infected NHPs (rhesus macaques, baboons, and marmosets). During the first 3 days, macaques developed clinical signatures of viral infection and systemic inflammation, coupled with early evidence of viral replication and mild-to-moderate interstitial and alveolar pneumonitis, as well as extra-pulmonary pathologies. Cone-beam CT scans showed evidence of moderate pneumonia, which progressed over 3 days. Longitudinal studies showed that while both young and old macaques developed early signs of COVID-19, both groups recovered within a two-week period. Recovery was characterized by low-levels of viral persistence in the lung, suggesting mechanisms by which individuals with compromised immune systems may be susceptible to prolonged and progressive COVID-19. The lung compartment contained a complex early inflammatory milieu with an influx of innate and adaptive immune cells, particularly interstitial macrophages, neutrophils and plasmacytoid dendritic cells, and a prominent Type I-interferon response. While macaques developed moderate disease, baboons exhibited prolonged shedding of virus and extensive pathology following infection; and marmosets demonstrated a milder form of infection. These results showcase in critical detail, the robust early cellular immune responses to SARS-CoV-2 infection, which are not sterilizing and likely impact development of antibody responses. Thus, various NHP genera recapitulate heterogeneous progression of COVID-19. Rhesus macaques and baboons develop different, quantifiable disease attributes making them immediately available essential models to test new vaccines and therapies.
Bismuth nanoparticles obtained by a facile synthesis method exhibit antimicrobial activity againstStaphylococcus aureusandCandida albicans
Authors: Vazquez-Munoz Roberto , Arellano-Jimenez M. Josefina , Lopez-Ribot Jose 
Published: June 2020

AbstractBismuth compounds are known for their activity against multiple microorganisms; yet, the antibiotic properties of bismuth nanoparticles (BiNPs) remain poorly explored. The objective of this work is to further the research of BiNPs for nanomedicine, particularly as a disinfectant and for future treatments. Stable PVP-coated BiNPs were produced by a chemical reduction process, in less than 30 minutes, in a heated alkaline glycine solution, by the chelation and reduction of the bismuth (III) ion; resulting in the generation of small, spheroid particles with a crystalline organization. We assessed the antibacterial and antifungal activity of bismuth nanoparticles. PVP-BiNPs showed potent antibacterial activity against the pathogenic bacteriumStaphylococcus aureusand antifungal activity against the opportunistic pathogenic yeastCandida albicans, both under planktonic and biofilm growing conditions. Our results indicate that BiNPs represent promising antimicrobial nanomaterials, and this facile synthetic method may allow for further investigation of their activity against a variety of pathogenic microorganisms.
Antecedents to responsible financial management behavior among young adults: moderating role of financial risk tolerance
Authors: Bapat Dhananjay 
Published: June 2020

PurposeThe study examines the antecedents of responsible financial management behavior among young adults in India and explores the role of financial risk tolerance as a moderating variable.Design/methodology/approachThe sample includes young adults in the age group of 18–35. The analysis uses a two-step approach via standard partial least squares structural modeling (PLS-SEM) and ordinary least square (OLS) regression.FindingsStructural modeling results show that financial attitude fully mediates the relationship between financial knowledge and responsible financial management behavior, and locus of control influences responsible financial management behavior. Financial risk tolerance moderates the relationship. Among demographic factors, age and occupation influence responsible financial management behavior.Research limitations/implicationsThe financial knowledge used in the survey are based on self-reported responses. The future study can include participants from both developed and emerging countries to assess similarities and differences.Practical implicationsDespite the growing focus on improving financial literacy, there are growing concerns regarding responsible financial behavior. Since financial services is related to fiduciary responsibility, managers and policymakers need to ensure that financial knowledge results in improving financial attitude, which further leads to responsible financial behavior.Originality/valueThe present study from an emerging country will add value to the literature.
Understanding early-stage firm performance: the explanatory role of individual and firm level factors
Authors: Seet Pi-Shen , Lindsay Noel , Kropp Fredric 
Published: June 2020

PurposeThis study presents and validates a theoretical model linking individual characteristics of the founding or lead innovative entrepreneur of a start-up venture – the entrepreneur's values, entrepreneurial attitudes and entrepreneurial self-efficacy – to the firm's entrepreneurial orientation (EO) and market orientation (MO) and, ultimately, to firm performance.Design/methodology/approachWe conducted a survey on a stratified random sample of founders of early-stage South Australian micro- and small enterprises with a response rate of 24% (N = 204). Structural equation modelling was used to evaluate the model.FindingsThe study found that there is a significant relationship between the individual lead entrepreneur and firm strategies developed in early-stage firms in explaining firm performance. It also found that internal values are positively related to entrepreneurial attitude. Entrepreneurial attitude is positively related to entrepreneurial self-efficacy and EO innovativeness. In turn, entrepreneurial self-efficacy is related to innovativeness, proactiveness and risk-taking. The proactiveness dimension of EO and entrepreneurial attitude is related to MO. Entrepreneurial self-efficacy, innovativeness and MO are related to firm performance.Research limitations/implicationsThis research was limited to entrepreneurial ventures in South Australia and may lack generalisability in other states and countries.Originality/valueThe research contributes to the understanding of the heterogeneity within self-employed individuals, in particular among innovative entrepreneurs, by expanding insights regarding antecedents and consequences of the entrepreneurial process. It develops insights into the links of individual-level constructs with firm-level constructs to develop a more meaningful understanding of new venture creation and performance. It enhances our knowledge of the heterogeneity within the group of self-employed by exploring the individual entrepreneurial antecedents of performance in early-stage firms.
A collaborative project delivery method for design of a high-performance building
Authors: Engebø Atle , Klakegg Ole Jonny , Lohne Jardar , Lædre Ola 
Published: June 2020

PurposeTo achieve the shift towards sustainable construction the industry must change the project delivery methods used. Therefore, this paper reports on a high-performance building project that implemented a collaborative project delivery method through the examination of the following research questions: RQ1: What were the most important contractual, cultural and organisational elements studied in the collaborative project delivery method? RQ2: What were the effects of the studied elements?Design/methodology/approachA longitudinal case study approach was adopted. As for means of data collection, a document review, semi-structured interviews, and observations was conducted. A total of 20 observations (App. 80 h, 175 pages of notes) and 12 interviews (App. 20 h, 100 pages of notes) with project participants was conducted.FindingsThe analysis indicates that if attention is paid to task, team and individual needs through contractual, cultural and organisational elements, this will affect the development of an integrated team.Research limitations/implicationsWe demonstrate that the effects of the elements are shown through their achievement in creating and sustaining an integrated team of inter-organisational participants working in a collaborative environment.Practical implicationsIt provides a better understanding of how a collaborative project delivery method for the design phase emphasises team integration. We demonstrate that while the principal sets the contractual boundaries by deciding the contractual elements, the agent should be intentional in the selection and use of organizational and cultural elements.Originality/valueThe paper suggests that it is not enough to just have contractual elements implemented. Consequently, this insight suggests that managers should be attentive to the untapped potential that lies within organisational and cultural elements.
Statistical quality control in the food industry: a risk-based approach
Authors: Prata Emille Rocha Bernardino de Almeida , Chaves José Benício Paes , Gomes Silvane Guimarães Silva , Passos Frederico José Vieira 
Published: June 2020

PurposeQuantitative metrics should be used as a risk management option whenever possible. This work proposes a framework for the risk quantification and the resulting risk-based design of control charts to monitor quality control points.Design/methodology/approachTwo quality control models were considered for the risk quantification analysis. Estimated operating characteristic curves, expressing the defect rate (on a ppm basis) as a function of the sample size, process disturbance magnitude and process capacity, were devised to evaluate the maximum rate of defective product of the processes. The proposed framework applicability on monitoring critical control points in Hazard Analysis and Critical Control Point (HACCP) systems was further evaluated by Monte Carlo simulations.FindingsResults demonstrate that the proposed monitoring systems can be tuned to achieve an admissible failure risk, conveniently expressed as the number of non-conforming items produced per million products, and these risks can be properly communicated. This risk-based approach can be used to validate critical control point monitoring procedures in HACCP plans. The expected rates of non-conforming items sent out to clients estimated through stochastic simulation procedures agree well with theoretical predictions.Practical implicationsThe procedures outlined in this study may be used to establish the statistical validity of monitoring systems that uses control charts. The intrinsic risks of these control systems can be assessed and communicated properly in order to demonstrate the effectiveness of quality control procedures to auditing third parties.Originality/valueThis study provides advancements toward practical directives for the implementation of statistical process control in the food industry. The proposed framework allows the assessment and communication of intrinsic failure risks of quality monitoring systems. It may contribute to the establishment of risk-based thinking in the constitution of quality management systems.
Government subsidies in the power battery recycling industry
Authors: Ding Peiqi , Zhao Zhiying , Li Xiang 
Published: June 2020

PurposeThe power battery is the core of a new energy vehicle and plays a vital role in the rise of the new energy vehicle industry. As the number of waste batteries increases, firms involved in the industry need to properly dispose them, but what party is responsible remains unclear. To reduce environmental impacts, governments introduce two subsidy policies, i.e. collection subsidies, which are provided to the collecting firms, and dismantling subsidies, which are provided to the dismantling firms.Design/methodology/approachBased on the different characteristics of the subsidies, we develop a stylized model to examine the collection strategies and the preferences over the subsidies.FindingsWe derive several insights from analysis. First, the collection strategies depend on the fixed collection cost. Second, the key factor determining the firm's subsidy preference is the efficiency of dismantling. Finally, if the primary target is the collection rate, governments prefer to provide collection subsidies. If consider the environmental impact, the choice of subsidies has to do with the efficiency of dismantling. Moreover, from a social welfare perspective, the raw material cost and the efficiency of dismantling are core indicators of decision.Originality/valueThis work develops the first analytical model to study two power battery subsidies and investigate the optimal collecting strategies and subsidy preferences. The insights are compelling not only for the manufacturer and the third party but also for policymakers.Peer reviewThe peer review history for this article is available at:https://publons.com/publon/10.1108/IMDS-08-2019-0450
An autoethnography of death and dying in Northern Ireland
Authors: Carroll Áine 
Published: June 2020

PurposeIn Northern Ireland, access to good quality palliative care is an accepted and expected part of modern cancer care. The “Transforming Your Palliative and End of Life Care” programme “supports the design and delivery of coordinated services to enable people with palliative and end of life care needs to have choice in their place of care, greater access to services and improved outcomes at the end of their lives”. The purpose of this autoethnography is to share the author’s lived experience so that it might be used to improve services.Design/methodology/approachAutoethnography is employed as the research method. The author describes her experience of caring for father over the last six months of his life. She explores the tensions between the different players involved in the care of her father and the family and the internal conflict that developed within her as daughter, carer, care coordinator and doctor. Using multiple data sources, selected data entries were explored through reflexive, dyadic interviews to explore the experience and meaning in each story.FindingsThe author found that autoethnography was a powerful tool to give voice to the carer experience. Narration can be a powerful tool for capturing the authentic lived experiences of individuals and families and is a tool seldom utilised in integrated care. This account provides an insight into the author's expectations of integrated palliative care, as a designer and implementer and now an academic in integrated care and concludes with some reflections about the gap between policy and practice in palliative care services in Northern Ireland.Originality/valueAutoethnography can be a powerful tool for capturing the authentic lived experiences of individuals and families and is an essential component of the quadruple aim.
Numerical and Experimental Study of Multiphase Transient Core-Annular Flow Patterns in a Spouted Bed
Authors: Zhou Ling , Han Chen , Bai Ling , Shi Weidong , Agarwal Ramesh 
Published: June 2020

Abstract
Dense solid–gas bubbling systems with combined fluid-particle motion are among one of the most extensively used fluidization forms used in the chemical industry. Therefore, it is important to have a good understanding of the hydrodynamic behavior of bubbles. In this paper, both the experiment and numerical simulations are used to investigate the flow patterns in a spouted bed. For numerical simulations, the bidirectional coupling simulations using computational fluid dynamics (CFD) with discrete element method (DEM) are conducted. The results show that the simulations can accurately predict the bubbles morphology compared with the experimental results. When the number of particles is 30,000, only a single core-annular flow pattern appears. When the number of particles is increased to 36,500, the single bubble in the spouted bed transitions into two and a double core-annular flow pattern emerges. As the number of particles is increased to 43,000, a complex multicore-annular flow pattern appears. These flow patterns are also observed in the experiments using high-speed imaging camera. This paper analyzes and explains the causes of these flow phenomena from the dynamic characteristics of particle phase and fluid phase. These results have great significance in providing guidance for optimization of dense phase bubbling spouted beds.