bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genome-wide identification of the amino acid permease genes and molecular characterization of their transcriptional responses to various nutrient stresses in allotetraploid rapeseed
Authors: Zhou Ting , Yue Cai-peng , Huang Jin-yong , Cui Jia-qian , Liu Ying , Wang Wen-ming , Tian Chuang , Hua Ying-peng 
Published: April 2020

Abstract
Background
Nitrogen (N), referred to as a “life element”, is a macronutrient essential for optimal plant growth and yield production. Amino acid (AA) permease (AAP) genes play pivotal roles in root import, long-distance translocation, remobilization of organic amide-N from source organs to sinks, and other environmental stress responses. However, few systematic analyses of AAPs have been reported in Brassica napus so far.

Results
In this study, we identified a total of 34 full-length AAP genes representing eight subgroups (AAP1–8) from the allotetraploid rapeseed genome (AnAnCnCn, 2n = 4x = 38). Great differences in the homolog number among the BnaAAP subgroups might indicate their significant differential roles in the growth and development of rapeseed plants. The BnaAAPs were phylogenetically divided into three evolutionary clades, and the members in the same subgroups had similar physiochemical characteristics, gene/protein structures, and conserved AA transport motifs. Darwin’s evolutionary analysis suggested that BnaAAPs were subjected to strong purifying selection pressure. Cis-element analysis showed potential differential transcriptional regulation of AAPs between the model Arabidopsis and B. napus. Differential expression of BnaAAPs under nitrate limitation, ammonium excess, phosphate shortage, boron deficiency, cadmium toxicity, and salt stress conditions indicated their potential involvement in diverse nutrient stress responses.

Conclusions
The genome-wide identification of BnaAAPs will provide a comprehensive insight into their family evolution and AAP-mediated AA transport under diverse abiotic stresses. The molecular characterization of core AAPs can provide elite gene resources and contribute to the genetic improvement of crop stress resistance through the modulation of AA transport.
Low lung function and the risk of incident chronic kidney disease in the Malmö Preventive Project cohort
Authors: Zaigham Suneela , Christensson Anders , Wollmer Per , Engström Gunnar 
Published: April 2020

AbstractBackgroundAlthough the prevalence of kidney disease is higher in those with reduced lung function, the longitudinal relationship between low lung function and future risk of chronic kidney disease (CKD) has not been widely explored.MethodsBaseline lung function was assessed in 20,700 men and 7325 women from 1974 to 1992. Mean age was 43.4 (±6.6) and 47.5 (±7.9) for men and women respectively. Sex-specific quartiles of FEV1and FVC (L) were created (Q4: highest, reference) and the cohort was also divided by the FEV1/FVC ratio (≥ or < 0.70). Cox proportional hazards regression was used to determine the risk of incident CKD events (inpatient or outpatient hospital diagnosis of CKD) in relation to baseline lung function after adjustment for various confounding factors.ResultsOver 41 years of follow-up there were 710 and 165 incident CKD events (main diagnosis) in men and women respectively. Low FEV1was strongly associated with future risk of CKD in men (Q1 vs Q4 adjusted HR: 1.46 (CI:1.14–1.89), p-trend 0.002). Similar findings were observed for FVC in men (1.51 (CI:1.16–1.95), p-trend 0.001). The adjusted risks were not found to be significant in women, for either FEV1or FVC. FEV1/FVC < 0.70 was not associated with increased incidence of CKD in men or women.ConclusionLow FEV1and FVC levels at baseline are a risk factor for the development of future incident CKD in men. Monitoring kidney function in those with reduced vital capacity in early life could help with identifying those at increased risk of future CKD.
The reliability and validity of a novel Chinese version simplified modified Rankin scale questionnaire (2011)
Authors: Yuan Junliang , Wang Yunxiao , Hu Wenli , Bruno Askiel 
Published: April 2020

Abstract
Background
The modified Rankin Scale (mRS) is a key global outcome measure after stroke internationally. The latest English version of the simplified modified Rankin scale questionnaire (smRSq)(2011) is a reliable and valid tool in scoring the mRS after stroke. In order to use this tool in Chinese patients, we translated it into Chinese and tested its clinimetric properties.

Methods
The English version smRSq (2011) was translated into Chinese by a standard process. We recruited 300 consecutive hospitalized ischemic stroke patients in the department of neurology, Beijing Chaoyang Hospital. Six randomly paired raters scored the conventional mRS, the novel Chinese version smRSq (2011), the National Institutes of Health Stroke Scale (NIHSS), and the Barthel index (BI) in-person. Inter-rater reliability and validity were assessed.

Results
Among the 300 ischemic stroke patients, mean age was 64.9 ± 12.1 years, and 220 (73%) were male. For inter-rater reliability of the smRSq (2011), the percent agreement among the paired raters was 87%, the kappa (κ) was 0.84 (95% CI, 0.79–0.88), and the weighted kappa (κw) was 0.96 (95% CI, 0.95–0.98). The percent agreement between the smRSq (2011) scores and the conventional mRS scores was 55%, κ = 0.47 (95% CI, 0.40–0.54), and κw = 0.91 (95% CI, 0.89–0.93). In construct validity testing, the Spearman’s correlation coefficients comparing the smRSq (2011) scores with the NIHSS and the BI scores were 0.83 (P < 0.001) and − 0.86 (P < 0.001), respectively.

Conclusions
Our results show good to excellent clinimetric properties of the novel Chinese version smRSq (2011) in scoring the mRS in Chinese stroke patients. Further validation in other clinical settings, including in communities and by remote methods in China is warranted.
Drinking patterns and hydration biomarkers among young adults with different levels of habitual total drinking fluids intake in Baoding, Hebei Province, China: a cross-sectional study
Authors: Zhang Jianfen , Zhang Na , Wang Yan , Liang Shuxin , Liu Shufang , Du Songming , Xu Yifan , He Hairong , Cai Hao , Ma Guansheng 
Published: April 2020

Abstract
Background
The purposes were to investigate the drinking patterns and hydration biomarkers among young adults with different levels of habitual total drinking fluids intake.

Methods
A cross-sectional study was conducted among 159 young adults aged 18–23 years in Baoding, China. Total drinking fluids and water from food were assessed by 7-day 24-h fluid intake questionnaire and duplicate portion method, respectively. The osmolality and electrolyte concentrations of the 24 h urine and fasting blood samples were tested. Differences in LD1 (low drinker), LD2, LD3 and HD (high drinker) groups, stratified according to the quartiles of total drinking fluids, were compared using one-way ANOVA, Kruskal-Wallis H test and chi-square test.

Results
A total of 156 participants (80 males and 76 females) completed the study. HD group had greater amounts of TWI (Total Water Intake), water from food, higher and lower contributions of total drinking fluids and water from food to TWI, respectively, than LD1, LD2 and LD3 groups (p < 0.05). Participants in HD group had higher amounts of water and water from dishes than participants in LD1, LD2 and LD3 groups (p < 0.05). No significant differences were found in the contributions of different fluids to total drinking fluids within the four groups (p > 0.05). The osmolality of urine was 59–143 mOsm/kg higher in LD1 than that in LD2, LD3 and HD group (p < 0.05). The percentage of participants in optimal hydration status increased from 12.8% in LD1 group to 56.4% in HD group (p < 0.05). HD and LD3 groups had 386~793 higher volumes of urine than that of LD1 and LD2 groups (p < 0.05). Differences were found in the concentrations of electrolytes among the four groups (p < 0.05). No significant differences were found in the plasma biomarkers (p > 0.05), with the exception of higher concentration of Mg in LD3 and HD groups than that in LD1 and LD2 groups (p < 0.05).

Conclusions
Participants with higher total drinking fluids had better drinking pattern and hydration status. Interventions should be undertaken to advise adults to have adequate total drinking fluids, in order to keep in optimal hydration status.

Trial registration
The registration number was ChiCTR-ROC-17010320, which was registered on the Chinese clinical trial registry.
Intrusion of episcleral buckles: report of two cases and brief review
Authors: Zarei Mohammad , Mahmoudi Alireza , Hadi Abdollah , Riazi-Esfahani Hamid 
Published: April 2020

Abstract
Background
The authors report two cases of the scleral buckles intrusion and erosion that presented many years after primary surgery with vitreous haemorrhage in one of them. Although the erosion/intrusion of a silicone scleral buckle (SB) is rare, it may have serious consequences and optimal management can be challenging. Therefore, this diagnosis should be considered if attributable signs and symptoms including vitreous haemorrhage occurred after scleral buckling. The authors briefly review the literature on clinical presentation and management of the episcleral silicone buckling erosion and intrusion.

Case presentation
Case 1: A 48-year-old woman with a history of scleral buckling for an inferior rhegmatogenous retinal detachment presented with visual loss in her right eye. A vitreous haemorrhage was observed. After Close observation, Partial resolution of haemorrhage revealed an intruded sponge segment in inferior vitreous cavity. Case 2: A 26-year-old man was referred for retinal evaluation. Twenty years earlier, he had undergone lensectomy for bilateral childhood cataract. Ten years ago, he had developed an aphakic RRD in the left eye. The detachment was managed with pars plana deep vitrectomy, endolaser, an encircling silicone band, and silicone oil injection. On examination an eroded band was noted.

Conclusion
Although the erosion/intrusion of a silicone episcleral buckle is rare, it may have serious consequences and optimal management can be challenging. Unnecessarily destructive techniques may predispose the eye to this complication and should be avoided. Patients who have a history of SB need lifelong follow-up and this diagnosis should be considered if attributable signs and symptoms occurred.
Virulence characterization of Klebsiella pneumoniae and its relation with ESBL and AmpC beta-lactamase associated resistance
Authors: Soltani Elghar , Hasani Alka , Ahangarzadeh Rezaee Mohammad , Pirzadeh Tahereh , Ahangar Oskouee Mahin , Hasani Akbar , Noie Oskouie Arezoo , Binesh Ehsan 
Published: April 2020

Background and Objectives: Trend analysis reveals that Klebsiella pneumoniae has witnessed a steep enhancement in the antibiotic resistance and virulence over the last few decades. The present investigation aimed at a comprehensive approach investigating antibiotic susceptibility including, extended spectrum beta-lactamase (ESBL) and AmpC β-lactamase (AmpC) resistance and the prevalence of virulence genes among the K. pneumoniae isolates.
Materials and Methods: Sixty-one K. pneumoniae isolates were obtained from various clinical infections. Antimicrobial susceptibility was performed by disk diffusion method. The Mast® D68C test detected the presence of ESBLs and AmpCs phenotypically, and later presence of ESBL and AmpC genes was observed by polymerase chain reaction (PCR). Multi- plex-PCR was performed to investigate various virulence genes.

CMY-2

Results: Amongst 61 K. pneumoniae isolates, 59% were observed as ESBL and 14.7% as AmpC producers. All ESBL

CTX-M-15

producers were positive for bla

CTX-M-15

, while bla

CTX-M-14

was observed in 54.1% isolates. The frequency of AmpC genes was

CMY-2

as follows: bla

CMY-2

(60.7%) and bla

DHA-1

(34.4%). The most frequent virulence genes were those encoding enterobactin and

DHA-1

lipopolysaccharide. Presence of mrkD was associated with bla

CMY-2

DHA-1

gene, while bla

significantly (p≤0.05) correlated

DHA-1

with the presence of iutA and rmpA virulence genes. bla

positive isolates had urine as a significant source, while bla

positive isolates were mainly collected from wound exudates (p≤0.05).
Conclusion: Our results highlight that ESBL and AmpC production along with a plethora of virulence trait on K. pneumoni- ae should be adequately considered to assess its pathogenesis and antibiotic resistance.
Influence of Lactobacillus casei WB 315 and crude fish oil (CFO) on growth performance, EPA, DHA, HDL, LDL, cholesterol of meat broiler chickens
Authors: Yulianto Andreas Berny , Lokapirnasari Widya Paramita , Najwan Rifqi , Wardhani Hana Cipka Pramuda , Rahman Nabil Fariz Noor , Huda Khoirul , Ulfah Nuria 
Published: April 2020

Background and Objectives: Use of antibiotics as growth promoters in animal feeds has been restricted due to the residues in poultry products such as egg and meat, furthermore to the antibiotic resistant of pathogenic bacteria. The prohibition of their use opens the opportunity for the use of non-antibiotic feed additives such as probiotics. The objectives of this study were to investigate the effect of the addition of Lactobacillus casei WB 315 and crude fish oil (CFO) to diets on growth performance, eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), low density lipoproteins (LDL), high density lipoprotein (HDL), and cholesterol levesl of broiler chickens.
Materials and Methods: In this research, one-day old male broiler chicks were used and divided equally into four groups, namely a basal diet without L. casei WB 315 and without CFO (P0), basal diet supplemented with 0.5% L. casei WB 315 of total broiler basal feed (1.2 × 109 cfu/ml) and without CFO (P1), basal diet supplemented without L. casei WB 315 and 1% CFO of total broiler basal feed (P2), and basal diet supplemented with 0.5% L. casei WB 315 of total broiler basal feed (1.2
× 109 cfu/ml) and 1% CFO of total broiler basal feed (P3) for 35 days.
Results: The results of addition 0.5% Lactobacillus casei WB 315 (1.2 × 109 cfu/ml) and 1% CFO of total broiler basal feed after 35 days showed significant difference among treatment in feed efficiency (p<0.05), feed conversion ratio (p<0.05), feed consumption (p<0.05), EPA (p<0.05), DHA (p<0.05), increase HDL (p<0.05), reduced the LDL (p<0.05), and reduce cholesterol (p<0.05) in meat broiler chicken.
Conclusion: It is concluded that the addition of L. casei WB 315 and crude fish oil (CFO) could significant improve the growth performance (feed efficiency, feed conversion ratio, feed consumption) and could significantly improve EPA, DHA and increase HDL and decrease LDL in meat poultry product.
The high efficacy of luliconazole against environmental and otomycosis Aspergillus flavus strains
Authors: Moslem Maryam , Mahmoudabadi Ali Zarei 
Published: April 2020

Background and Objectives: Luliconazole is currently confirmed for the topical therapy of dermatophytosis. Moreover, it is found that luliconazole has in vitro activity against some molds and yeast species. The aim of the present study was to evaluate the efficacy of luliconazole in comparison to routine used antifungals on clinical and environmental isolates of Aspergillus flavus.
Materials and Methods: Thirty eight isolates of A. flavus (18 environmental and 20 clinical isolates) were detected based
on morphological and microscopic features and also PCR-sequencing of β-tubulin ribosomal DNA gene. All the isolates
were tested against luliconazole, voriconazole, amphotericin B and caspofungin. Minimum inhibitory concentration (MIC),

90

MIC50, MIC
isolates.

and MIC Geometric (GM) were calculated using CLSI M38-A2 protocol for both environmental and clinical

GM

Results: Luliconazole with extremely low MIC range, 0.00049-0.00781 μg/mL and MIC

0.00288 μg/mL showed very

strong activity against both clinical and environmental A. flavus isolates. Moreover, voriconazole inhibited 100% of isolates at defined epidemiological cutoff values (ECV ≤ 2 µg/ml). 50% and 27.8% of clinical and environmental isolates of A. flavus, were resistant to caspofungin, respectively. Whereas, all the isolates were found to be resistant to amphotericin B.

GM

Conclusion: The analysis of our data clearly indicated that luliconazole (with MIC

0.00244 µg/ml for clinical and 0.00336

μg/ml for environmental isolates) had the highest in vitro activity against A. flavus strains.
Cesarean Scar Ectopic Pregnancy: The Lurking Danger in Post Cesarean Failed Medical Abortion
Authors: Anant Monika , Paswan Anita , Chandrajyoti Chandrajyoti 
Published: April 2020

Cesarean scar pregnancy (CSP) is a potentially life threatening ectopic pregnancy where a missed diagnosis is commoner than an accurate diagnosis. Incidence of Ectopic pregnancy is 1 – 2 % and cesarean scar ectopic occurs in about (0.05%) 1 in 2000 of all pregnancies. With increasing cesarean section rates worldwide, CSP is bound to increase with its dreaded complications like uterine rupture and catastrophic hemorrhage. Three patients misdiagnosed as incomplete miscarriages in post cesarean pregnancies in other centers were found to be CSP in Gynaecology department of a tertiary level hospital. All three patients were managed successfully, two surgically and one medically.
Evaluation of Dimensional Accuracy of 3D printed mandibular model using two different Additive Manufacturing Techniques based on Ultra-Low Dose Multislice Computed Tomography scan data
Authors: Hesham Noran Mohamed , Kandel Hossam , Dakhli Iman Ismail 
Published: April 2020

Abstract
Article Type : Research article Corrective and reconstructive cranio-maxillofacial interventions are a challenging area of surgery that requires careful pre-operative planning. To accommodate the need for precision pre-operative planning, surgeons frequently need guidance such as a 3D model to display complex cranial structures. 3D model is a manufactured model made by a 3D printer using digital imaging and communication for medicine (DICOM) data from a CT scan, and then, converted into computer-assisted design (CAD) data. Image acquisition is a very important step in generation of 3D objects as the quality of the object depends on the quality of the data. MSCT is widely applied for rapid prototyping because image post-processing is less complex for MSCT data.This aim of the present study is to evaluate the dimensional accuracy of the 3D printed mandibular models fabricated by two different additive manufacturing techniques using highly precise one as selective laser sintering (SLS) and low-cost one as fused filament fabrication and whether they are both comparable in terms of precision.In this diagnostic accuracy study , 7 mandibular models will be recruited for the study, 10 linear measurements will be determined on the models. MSCT scanning of the model will be performed. afterwards 3d printing of the scanned image will be done using SLS and FFF 3d printers. the predetermined 10 linear measurement will be measured on the printed models to be compared with the reference standard measurement to determine the accuracy of the 3d printers in dental applications like surgical guides, orthodontic appliances, fixed prosthodontics appliances and other many dental applications. The study is scheduled to be done by october 2020Article Type : Protocol for diagnostic accuracy study
Effects of Job Demands, Job Resources, and Workaholism on Overwork: A Cross-Sectional Survey in China
Authors: WANG XIN , YANG Jing , NAGAI Yoritoshi 
Published: April 2020

Abstract
Background Overwork has become the common for some employees in China. The ‘Healthy China 2030’ plan mandates prevention of excessive overtime, making serious overwork was not advocated. Meeting new regulations requires clarification of the factors influencing overwork; however, Chinese researchers have not thoroughly examined ‘overwork’ and few proven models are available for addressing its relationship to job demands, job resources, and workaholism. Methods This study analysed these factors and overwork among employees in Chinese companies. A total of 1047 employees completed the Workers Fatigue Accumulated Degree by Self-Diagnostic Questionnaire (WFAD-SQ) for Assessment of Workers' Accumulated Fatigue to measure the degree of overwork; Workaholism Battery (WorkBAT) was applied to evaluate workaholism; the Job content qualifications (JCQ) were adopted to survey job demands–resources. Results Structural equation modelling (SEM) verified the effects shown, indicating job resources significantly negatively affect and job demands positively affect overwork. Workaholism demonstrated a significant intermediary role between job demands-resources and overwork. The SEM results indicate job demands-resources have both direct and indirect effects on overwork, and task demands, psychological requirements, and colleague support are the primary positive protective predictors for overwork. Conclusions The findings indicate the importance of employers strictly enforcing overtime rules, ensuring staffs’ rights for rest, and establishing harmonious working relationships between employees.
Responsiveness of the EuroQoL 5-Dimension (EQ-5D) questionnaire in patients with spondyloarthritis
Authors: Tsang Helen Hoi Lun , Wong Carlos King Ho , Cheung Prudence Wing Hang , Lau Chak Sing , Chung Ho Yin , Cheung Jason Pui Yin 
Published: April 2020

Abstract
Background Spondyloarthritis (SpA) has a significant impact on patients’ quality of life due to functional impairments. Generic health instruments like the EuroQoL 5-dimension (EQ-5D) is important for cost-utility analysis of health care interventions and calculation of quality-adjusted life-years. It has been validated in patients with SpA. However, its responsiveness property has yet to be determined. Hence, the aim of study is to test the responsiveness properties of the EQ-5D health measure for Chinese patients with SpA.Methods Prospective and consecutive recruitment of 220 Chinese patients with SpA was conducted with follow-up assessments 6 months later. Demographic data including smoking and drinking habits, education level, income and occupation was collected. Disease-associated data including disease duration, presence of back pain, peripheral arthritis, dactylitis, enthesitis, uveitis, psoriasis, and inflammatory bowel disease was also recorded. Questionnaires regarding disease activity and functional disability (BASDAI, BASFI, BASGI, BASMI, ASDAS, ODI), mental health (HADS depression and anxiety) and the EQ-5D scores were recorded. Responsiveness was tested against the global rating of change scale (GRC) and changes in disease activity using BASDAI and ASDAS.Results A total of 112 (50.9%) patients completed the follow-up assessments. Most patients (63.4%) had low disease activity level with BASDAI <4 and 50.9% of patients had inactive disease by ASDAS-CRP. EQ-5D scores was well discriminated between improved and worsened BASDAI scores. The GRC was not able to discriminate adequately. No significant ceiling or floor effect was observed. Conclusions The EQ-5D demonstrates satisfactory responsiveness property for assessment of changes in disease activity in patients with SpA.