bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of a natural plant ingredient — Pulsatilla saponin B4 on clinical mastitis and serum inflammatory indices in dairy cows
Authors: Shen Liuhong , Qian Bolin , Lv Shangkui , You Liuchao , Zhang Yue , Shen Yu , Xiao Jinbang , Su Zhetong , Dong Ke , Zong Xiaolan , Yu Shumin , Cao Suizhong , Yang Shilin , Feng Yulin 
Published: August 2020

Abstract
Background

To investigate the effect of Pulsatilla saponin B4, a purified extract form Pulsatilla Chinensis, on the treatment of clinical mastitis in dairy cows, 12 healthy cows were used as the control group (group A, no treatment), and 36 cows with mastitis were divided into 3 groups based on the quaque die (QD) intramuscular injection (IM) dose used in the as follows, group B (15 mL), group C (30 mL), group D (60 mL). Identified the microorganisms in milk during the experiment,samples in test groups were cultured, isolated, and verified by quantitative real-time polymerase chain reaction (qPCR). Somatic cell count (SCC) and serum inflammatory indices in cows were measured with a 1-day interval in group C and compared with group A.
Results

Results showed that bacterial detection rates were 100% (group B), 83.33% (group C) and 100% (group D). After the treatment bacterial detection rates dropped to 50.00% (group B), 50.00% (group C), and 41.67% (group D). The medicine was effective in all test groups and showed a dose-effect relationship. Serum haptoglobin (HP), prostaglandin E2 (PGE2), interleukin-1 alpha (IL-1α), interleukin-1 beta (IL-1β), interleukin-2 (IL-2), interleukin-8 (IL-8), interleukin-10 (IL-10) and tumour necrosis factor alpha (TNF-α), interleukin-4 (IL-4), leukotriene B4 (LTB4) also the SCC were significantly higher than healthy cows (P < 0.05) on the day (d) 1 in group C but had no significant difference with group A after treatment.
Conclusion

Treatment with Pulsatilla saponin B4 relieved mastitis, leading to relieve of bacterial infection, SCC and levels of serum inflammatory factors effectively in cows with mastitis after being administered for 4–6 days QD.
Practical lineshape of a laser operating near an exceptional point
Authors: An Kyungwon , Kim Jinuk , Kim Juman , Seo Jisung , Park Kyu-Won , Songky Moon 
Published: August 2020

Abstract
We present a practical laser linewidth broadening phenomenon in the viewpoint of high sensitivity of an exceptional point (EP). A stochastic simulation model is implemented to describe the fluctuations in the cavity resonance frequencies. The linewidth originated from external noises are maximized at the EP. The linewidth enhancement factor behaves similarly to the Petermann factor although the Petermann effect is not considered. In the long coherence time limit, the power spectral density of the laser exhibits a splitting in the vicinity of the EP although the cavity eigenfrequencies coalesce at the EP.
Linking Technology to Address the Social and Medical Determinants of Health for Safer Use of Medicines
Authors: Woosley Raymond Leon , Simmons June , Sefilyan Ester M. , Atkins Sandra K. , Black Kristin , Read William A. 
Published: August 2020

Abstract
Background: Both medical and social issues can negatively affect health outcomes, especially for vulnerable populations. Assessing risk from these two perspectives independently, two non-profit organizations each developed a decision support program to identify medication-risk that could negatively impact health outcomes. This raises the question, could a combined program have greater potential for improved outcomes.Methods: HomeMeds℠ (HM), a social health program in which trained social services staff make home visits to vulnerable clients, was combined with MedSafety Scan (MSS), a medical health, clinical decision support tool. Data captured in the home visit were entered into the HM and MSS programs to analyze each patient’s drug risk profile and their list of medicines to detect those patients at greatest risk of adverse health outcomes caused by medications. Results: One hundred and eight patients (mean age 77 with multiple co-morbidities and LACE+ score >29) received a post-discharge home visit by trained social services staff. The number of drugs reported as being taken was 10.4 ± 5.1 (range 1-26) which was less than prescribed at discharge in 62% of patients (range 1-8). Both programs detected serious risk of medication-induced harm, mostly from different causes. HomeMeds detected medically relevant events such as unreported falls (24), dizziness (37), confusion (27) and uncontrolled pain (37). MedSafety Scan identified 22 patients with moderate to very high risk of cardiac arrhythmia due to their medicines. In 65% of patients, an average of 6.2 ± 5.6 potentially serious drug interactions were detected by MedSafety Scan but not HomeMeds.Conclusions: Combined analyses of data from these two decision support programs that approach health issues and patient needs from different perspectives yielded complementary findings that address both medical and social determinants of health after hospital discharge. These have the potential to reduce medication-induced harm, costly re-hospitalization and/or emergency room visits. The results of this quality improvement project are encouraging and support the further evaluation of this combined approach in other vulnerable populations such as the seriously mentally ill, frail, those confined to home, opiate-dependent or otherwise impaired.
Development of PREPARE (PREdicting PAtients’ Long-Term Outcome for REcovery): A Practically Usable Prediction Model for Quality of Life of ICU Survivors
Authors: Wubben Nina , Boogaard Mark van den , Ramjith Jordache , Bisschops Laurens LA , Frenzel Tim , Hoeven Johannes G van der , Zegers Marieke 
Published: August 2020

Abstract
The authors have withdrawn this preprint due to erroneous posting.
Effect of Intensive Care Provided by Nurse Practitioners for Postoperative Patients: A Retrospective Observational Before-and-after Study
Authors: Mori Kazunao , Makino Satoshi , Takabayashi Takuya , Kurosawa Masahiro , Ohashi Wataru , Tsukamoto Yoko , Okumura Masatoshi , Fujita Yoshihito , Fujiwara Yoshihiro 
Published: August 2020

Abstract
Background Since April 2016, nurse practitioners (NPs) have provided inter-professional team collaborative care for postoperative patients in our intensive care unit (ICU). To assess the effectiveness of NP practice, we compared patient outcomes with and without NP care in our ICU. Methods This retrospective observational cohort study was undertaken at Aichi Medical University Hospital in Japan from 01 April 2015 and 31 March 2017. Mechanically-ventilated postoperative patients who stayed in the ICU for >2 days were eligible for inclusion. We compared the patient outcomes between the period without NP care from April 2015 to March 2016 (physician group) and the period with NP care from April 2016 to May 2017 (NP-physician group). The primary outcome was ICU length of stay. Secondary outcomes were mechanical ventilation days and total hospital length of stay. To further determine the factors associated with ICU length of stay, multiple regression analysis was performed for age, Acute Physiology and Chronic Health Evaluation (APACHE) II score, NP-physician group, and physician group. Results A total of 387 patients were included in the study. Among these patients, 213 were assigned to the NP-physician group and 174 were assigned to the physician group. The NP-physician group had shorter ICU length of stay than the physician group (4.8 ± 4.8 days versus 6.7 ± 10.3 days, p < 0.021). There were no significant differences between the two groups in mechanical ventilation days or total hospital length of stay. In the multiple regression analysis for ICU length of stay, APACHE II score and NP-physician group were associated with ICU length of stay. Specifically, NP-physician group reduced ICU length of stay by 2.6 days ( p = 0.003), and APACHE II score one-point decrease reduced ICU length of stay by 0.2 days ( p = 0.012). Conclusions The present study demonstrated shorter ICU length of stay in patients with NP care and a significant association between NP participation in the ICU team and ICU length of stay.
Intraoperative Fluid Overload Predicted Postoperative Debridement in Major Sacrum Tumor Resection: A Retrospective Case-control Study
Authors: Zhao Hong , Song Yingchao , Dong Sen , Feng Yi 
Published: August 2020

Abstract
Background Sacrum tumor resection is with high morbidity due to complex anatomy, sacral nerves involvement, massive bleeding and tumor malignancy. Risk factors related with complications following sacrectomy were not clearly defined.Method Anesthetic database of Peking University People’s Hospital, Beijing, China was searched for all patients (aged 14-70 years of age) received sacrum tumor surgery from 2014 to 2017. As part of the bleeding control program, intra-aortic balloon occlusion (IABO) was applied to patients whose tumor volume was more than 200 cm3, a tumor that had invaded cephalad to the S2-S3 disc space, or tumor with an abundant blood supply. Results Finally 355 patients who underwent sacrectomy were included in this study, among whom 278 patients received intraoperative IABO, whose duration of aortic occlusion was 72±33 min. Extensive hemorrhage (>2000 ml) occurred in 61(21.9%) patients receiving IABO. Fifty-six patients in IABO Group required postoperative debridement due to wound infection. The independent risk factor identified by logistic regression was fluid excess (calculated as volume infused (crystalloid, colloid, CRC and FFP), minus blood loss and urine output, divided by body weight (kg)), and decision tree analysis found that the cut-off point for fluid excess was 38.5 ml/kg. Then propensity score matching of intraoperative blood loss and aortic occlusion duration was adopted for patients whose fluid excess >38.5 ml/kg and those whose was lower or equal. Afterwards, 91 pairs of patients were generated. Fluid excess was significantly different (46 vs. 30 ml/kg, P=0.000) for patients whose fluid excess was >38.5 ml/kg, and required more postoperative debridement (24 (26.3%) vs. 12 (13.1%), P=0.000) than those whose was lower or equal. Conclusion In this retrospective cohort study about sacrum tumor resection, duration of aortic occlusion and anesthesia were identified as predictors for massive bleeding. Fluid overload was related with high morbidity and studies are needed to further improve clinical prognosis.
Prediction of Subclinical Chorioamnionitis After Cervical Cerclage
Authors: Li Li , PAN MIAN , Yan Jianying , Huang Xinxin , Qiu Liyin , Ma Mei , Zhang Jun 
Published: August 2020

Abstract
Objective: To explore non-invasive indices for predicting subclinical chorioamnionitis following cervical cerclage. Methods: We performed a retrospective analysis of 80 singleton pregnant women who underwent cervical cerclage surgery in our hospital. Eighty patients were divided into either a histological chorioamnionitis group (n=57) and non-histological chorioamnionitis group (n=23). Gestational age before cervical cerclage, cervical dilation size, vaginal microbiota, cervical microbial colonization, and inflammatory indicators related to peripheral blood cells (white blood cell count, neutrophil count, lymphocyte count, platelet count, CRP, neutrophil/lymphocyte ratio, platelet/white blood cell ratio, etc.) at pre-surgery time and onset of labor post-surgery were compared, and an independent sample t-test and multivariate logistic regression analysis were performed to study the risk factors associated with histological chorioamnionitis. Histological chorioamnionitis was used as the outcome variable, and receiver operating characteristic (ROC) curve analysis was performed to determine the cut-off value and evaluate the predictive value of these indicators for chorioamnionitis. Results: 1) Single-factor logistics analysis showed that the preoperative number of platelets, indicators related to peripheral blood cells during onset of labor post-surgery（the total number of leukocytes，neutrophils，platelets， platelet/white blood cell ratio） , the area under the curve of each indice was all statistically significant. Their cut-off values ​​were 229.5×109/L; 10.245×109/L; 9.86×109/L; 240.5×109/L; 1.022 respectively. 2) Additional multivariate logistic regression analysis was performed. Preoperative number of platelets, the number of white blood cells and platelet/white blood cell ratio during onset of labor were identified as significant variables. 3) The statistically significant variables (preoperative platelets, white blood cells and platelet/white blood cell during onset of labor) obtained from multi-factor analysis were grouped according to the cut-off value. A joint screening system was established that specified that two or more indicators are positive as joint screening, with a screening sensitivity of 87.5%, and specificity of 45.5%. Conclusions: After cervical cerclage, the number of white blood cells and the ratio of platelet/white blood cell during onset of labor combined with preoperative number of platelets have predictive value for potential histological chorioamnionitis in pregnant women with cervical cerclage.
13 year old Female with Progressive Pseudorheumatoid Dysplasia misdiagnosed as Juvenile idiopathic arthritis
Authors: Omar Anjumanara Anver , Ahmed Salman , Rodrigues John Chris , Owino Lawrence 
Published: August 2020

Abstract
Background: Juvenile idiopathic arthritis (JIA) is the most common chronic rheumatic disease in childhood and is of unknown aetiology. Progressive pseudorheumotoid dysplasia is a rare, autosomal recessively inherited, non-inflammatory musculoskeletal disorder caused by mutations occurring in the WISP3 gene. Joint cartilage is the primary site of involvement, leading to arthralgia, joint stiffness, contractures, enlargement of the epiphyses and metaphysis of the hand joints, spinal abnormalities, short stature, early osteoarthritis, and osteoporosis. The clinical features resemble juvenile idiopathic arthritis (JIA), and patients with PPD are usually misdiagnosed as JIA.Case Presentation: A 13 year old female presented to the rheumatology clinic with history of joint pains, bone pains and bone deformity for 5 years. Weight and Height were below the 3rd centiles. There was none tender swelling of metacarpophalangeal and interphalangeal joints and with scoliosis. Radiographs of the hands revealed widening of the epiphyses. With these findings Progressive Pseudorhematoid Dysplasia was suspected and genetic testing for WISP1, WISP2 and WISP3 was requested. A homozygous, likely pathogenic, variant was identified in WISP3 gene which confirmed our diagnosis.Conclusion: Progressive Pseudorhematoid Dysplasia is a rare form of spondylo-epi-metaphyseal dysplasia and clinically misdiagnosed as Juvenile Idiopathic arthritis. It’s important to consider PPD especially in patients with normal inflammatory markers who are being followed up for arthritis and not improving on anti-rheumatic intervention.
Focal Maculopathy in the Senile Cataract Population
Authors: Zhang Mei , Yuan Kun , Liu Jingwen , Zheng Haoran 
Published: August 2020

Abstract
Objective: Optical Coherence tomography (OCT) was employed to screen for maculopathy in the senile cataract population, investigate its incidence, and establish a healthy mode of management for maculopathy.Methods: A visual examination, slit-lamp microscope examination, direct ophthalmoscopic fundus examination, and a macular OCT examination were performed on 102 people with senile cataracts who were over 60 years in our hospital from January 1, 2019, to July 31, 2019. The demographic characteristics such as sex, age, physical examination mode: organization/individual, and routine physical examination items: presence or absence of hypertension, body mass index (BMI), blood biochemistry, total cholesterol, triglyceride (TG), fasting blood glucose and others.Results: Of the 102 subjects in the study, 28 were positive for maculopathy according to the OCT examination. Univariate analysis found that there were statistical differences between sexes and the presence of maculopathy (p<0.05). There were no significant differences in age, visual acuity, hypertension, BMI, fasting blood glucose, TG, total cholesterol, and cataract type (P>0.05). Multivariate logistic regression analysis, including all factors that might affect maculopathy, indicated that positivity for maculopathy and age (OR =2.549, 95%CI: 1.129–5.756, p<0.05) and gender (OR=3.907, 95%CI: 1.241–12.302, p<0.05) were related.Conclusion: The incidence of maculopathy in the senile cataract population was higher than that in the elderly population without cataract disease, and the proportion is 27.45%. Screening for maculopathy in the senile cataract population, especially among the elderly and females, should be improved.
CSF FAM3C- a possible biomarker of Alzheimer’s disease
Authors: Bian Chunmei , Li Wei , Wang Liping , Long Zhenzhen , Xue Ping , Guan Siming 
Published: August 2020

Abstract
Backgrounnd: The impact of Alzheimer's disease (AD) on the living quality and life expectancy has becoming increasingly severe, but the pathogenesis of AD is still being studied .This study aimed to investigate whether Family with sequence similarity 3 member C (FAM3C), which has been reported to be possibly associated with cognitive function, was associated with the incidence of AD.Methods: A total of 282 participants from Alzheimer’s Disease Neuroimaging Initiative (ADNI) were included. The demographic data, cerebrospinal fluid (CSF) FAM3C content, neuropsychological scores, CSF amyloid protein level, CSF tau protein level, and imaged data were collected. One-way analysis of variance and chi-square test were used to compare demographic data, neuropsychological test scores, CSF protein levels, and imaging data of three groups. Partial correlation analysis and multiple linear regression analysis were used to investigate the relationship between FAM3C and AD diagnostic indicators in different dimensions. An ordered multi-classification logistic regression model was established to determine whether FAM3C is related to the onset of AD. Results: Lower levels of FAM3C were observed in AD and mild cognitive impairment (MCI) groups. CSF FAM3C level was related to whole-brain atrophy and temporal lobe atrophy, worse cognitive performance, decreased amyloid β 1-42 (Aβ1-42), and lower regional cerebral glucose metabolism. Conclusion: CSF FAM3C might be a potential diagnostic biomarker of AD. Further study on concrete mechanisms may contribute to early diagnosis and treatment of AD.
RNA-Seq profiling revealed PBMC RNA as potential biomarker for hepatocellular carcinoma
Authors: Han Zhiyi , Feng Wenxing , Hu Rui , Ge Qinyu , Ma Wenfeng , Zhang Wei , Xu Shaomin , Zhan Bolin , Zhang Lai , Sun Xinfeng , Zhou Xiaozhou 
Published: August 2020

Abstract
Background

Hepatocellular carcinoma is one of the most common malignancies with extremely high incidence and mortality rates. Although there have been many studies focus on biomarkers study, few have been reported on PBMC RNA profiles of hepatocellular carcinoma.
Methods

In this study, we attempted to profile the expression of Peripheral Blood Mononuclear Cells (PBMCs) RNA by using RNA-seq technology and compared the transcriptome between hepatocellular carcinoma patients and the healthy controls. 17 patients and 17 healthy controls involved in this study, PBMCs RNA were sequenced. The sequencing data were analyzed with bioinformatics tools and qRT-PCR was used for selected differential expressed gene validation.
Results

It is showed that 1578 dysregulated genes found including 1334 upregulated genes and 244 downregulated genes. GO enrichment and KEGG analysis denoted most of the differential expressed genes (DEGs) involved in immune response are closely related to hepatocellular carcinoma. Expression of the 6 selected genes (DEGs, SELENBP1, SLC4A1, SLC26A8, HSPA8P4, CALM1, and RPL7p24) were confirmed by qRT-PCR, and higher sensitivity and specificity obtained by ROC analysis of the 6 genes. CALM1 was found gradually decreasing along with the tumor enlarged.
Conclusions

It is suggested potential biomarker for diagnosis, classification and therapeutic target of hepatocellular carcinomas. This study provided new visions into development of liver cancer and potential efficient clinical diagnosis in the future.
Association of Immunohistochemical Markers of Tumor Subtype with Response to Neoadjuvant Chemotherapy and Survival in Patients with Muscle-Invasive Bladder Cancer
Authors: Razzaghdoust Abolfazl , Ghajari Mahdi , Torbati Peyman Mohammadi , Jafari Anya , Salahi Maryam , Mofid Bahram , Basiri Abbas 
Published: August 2020

Abstract
Background: Clinical application of a readily accessible biomarker for identification of bladder cancer patients most likely to respond to neoadjuvant chemotherapy (NAC) could help clinicians avoid unnecessary chemotherapy and prevent its subsequent complications in patients unlikely to drive clinical benefit. The primary objective of this study was to investigate the association of immunohistochemical markers of tumor subtype with response to NAC and survival of muscle-invasive bladder cancer (MIBC) patients. Methods: MIBC patients treated with NAC in two tertiary referral hospitals were retrospectively included in the study. The tissue microarrays (TMAs) were assembled from transurethral resection of bladder tumor (TURBT) specimens and immunohistochemistry (IHC) was performed on the TMA slides. The association of independent variables, including singular IHC markers, combined IHC markers, and clinical covariates with clinical complete response (CR) to NAC, and overall survival (OS) was assessed using logistic regression and Cox proportional hazard regression analysis, respectively. Kaplan-Meier curves were plotted for different IHC-based subtypes. Results: Data from 140 MIBC patients treated with NAC were retrospectively reviewed. A total of 63 patients with available TURBT specimen were eligible to be included in the analysis. Our results showed that the IHC signature of KRT5/6(+)/KRT20(-), as a combined marker of basal subtype, was the only covariate significantly associated with the CR to NAC (p = 0.037). After a median follow-up of 41 (range, 12-76) months, no statistically significant differences in OS were found between different IHC-based subtypes (Log rank P = 0.721). In Cox proportional hazard regression analysis, age >65yr was independently associated with poorer OS after NAC (hazard ratio [HR], 2.26; 95% confidence interval [CI], 1.02–5.05), but failed to remain significant after adjusting for creatinine clearance (HR, 1.54; 95% CI, 0.58–4.10).Conclusion: The IHC expression of KRT5/6 and KRT20, as a readily accessible combined marker may help us to identify patients most likely to benefit from chemotherapy. The clinical utility needs to be established in larger prospective studies.