bluuebunny/crossref_metadata_2025_split huggingface dataset:
Efficacy of broflanilide (VECTRONTM&nbsp;T500) a new meta-diamide insecticide, for indoor residual spraying against pyrethroid-resistant malaria vectors.
Authors: Ngufor Corine , Govoetchan Renaud , Fongnikin Augustin , Vigninou Estelle , Syme Thomas , Akogbeto Martin , Rowland Mark 
Published: December 2020

Abstract
The rotational use of insecticides with different modes of action for indoor residual spraying (IRS) is recommended for improving malaria vector control and managing insecticide resistance. Insecticides with new chemistries are urgently needed. Broflanilide is a newly discovered insecticide under consideration. We investigated the efficacy of a wettable powder (WP) formulation of broflanilide (VECTRON T500) for IRS on mud and cement wall substrates in laboratory and experimental hut studies against pyrethroid-resistant malaria vectors in Benin, in comparison with pirimiphos-methyl CS (Actellic 300CS). There was no evidence of cross-resistance to pyrethroids and broflanilide in CDC bottle bioassays. In laboratory cone bioassays, broflanilide WP treated substrates killed >80% susceptible and pyrethroid-resistant An. gambiae sl for 6-14 months. At application rates of 100mg/m2 and 150 mg/m2, mortality of wild pyrethroid-resistant An. gambiae sl entering experimental huts in Covè, Benin treated with VECTRON T500 was similar to pirimiphos-methyl CS (57%-66% vs. 56%, P>0.05). Throughout the 6-month hut trial, monthly wall cone bioassay mortality on VECTRON T500 treated hut walls remained >80%. IRS with broflanilide shows potential to significantly improve the control of malaria transmitted by pyrethroid-resistant mosquito vectors and could thus be a crucial addition to the current portfolio of IRS insecticides.
Determinants of clinical practice guidelines’ utilization for the management of musculoskeletal disorders: A scoping review
Authors: Sorondo Delphine , Delpierre Cyrille , Côté Pierre , Salmi Louis-Rachid , Cedraschi Christine , Taylor-Vaisey Anne , Lemeunier Nadège 
Published: December 2020

Abstract
CONTEXT: Many clinical practice guidelines have been developed for the management of musculoskeletal disorders (MSDs). However, there is a gap between evidence-based knowledge and clinical practice, and reasons are poorly understood. Understanding why healthcare providers use clinical practice guidelines is essential to improve their implementation, dissemination, and adherence.AIM: To identify determinants of clinical practice guidelines’ utilization by health care providers involved in the assessment and management of MSDs.METHOD: A scoping review of the literature was conducted. Three databases were searched from inception to December 2019. Article identification, study design, methodological quality, type of healthcare providers, MSDs, barriers and facilitators associated with guidelines’ utilization were extracted from selected articles.RESULTS: 7667 citations were retrieved, and 43 articles were selected. 51% of studies were from Europe, and most were quantitative studies (64%) following a cross-sectional design (88%). Almost 80% of articles dealt with low back pain guidelines, and the most studied healthcare providers were general practitioners or physiotherapists. Five main barriers to guideline utilization were expressed by providers: 1) disagreement between recommendations and patient expectations; 2) guidelines not specific to individual patients; 3) unfamiliarity with “non-specific” term, or with the bio psychosocial model of MSDs; 4) time consuming; and 5) heterogeneity in guideline methods. Four main facilitators to guideline utilization were cited: 1) clinician’s interest in evidence-based practice; 2) perception from clinicians that the guideline will improve triage, diagnosis and management; 3) time efficiency; and 4) standardized language.CONCLUSION: Identifying modifiable determinants is the first step in developing implementation strategies to improve guideline utilization in clinical practice.
Pregnancy After Liver Transplant: Maternal and Perinatal Outcomes
Authors: Marzec Izabela , Słowakiewicz Aleksandra , Gozdowska Jolanta , Tronina Olga , Pacholczyk Marek , Lisik Wojciech , Durlik Magdalena 
Published: December 2020

Abstract
Background: Liver transplantation is a life-saving and successful therapeutic procedure which is more and more frequent worldwide, also among women of reproductive age. Consequently, there is an increasing number of reports of pregnancy following liver transplantation, but doubts still exist regarding preconception counseling and the the optimal method of managing pregnancy. The aim of this study was to report and evaluate pregnancy outcomes in women who had undergone liver transplantation.Methods: We retrospectively analyzed female patients after orthotopic liver transplantation who reported pregnancy and were under medical care of a single transplant center.Results: We identified 14 pregnancies in 10 women who had undergone liver transplantation (12 childbirths, one induced abortion due to fetal death in the first trimester, one pregnancy is still ongoing). Causes of transplantation include congenital or acquired disorders and the most common indication was autoimmune hepatitis (50%). The mean age at the point of transplantation was 28.5 (range 21–36), mean maternal age at pregnancy was 32 (range 26-43), and transplant-to-pregnancy interval was 4.07 years (range 1,5–7). The mean gestational week was 36.67 (range 31–40). Immunosuppression was maintained with combinations of prednisone (n=11), tacrolimus (n=13), and azathioprine (n=8) prior to and during pregnancy. Two pregnancies were unintended, so women took mycophenolate mofetil in the first weeks of gestation. Another two women stopped taking azathioprine due to increasing anemia. Maternal complications included increase of aspartate transaminase and alanine transaminase (n=2), anemia (n=4) and hyperthyroidism (n=2). Among the 12 childbirths, five (41,67%) were preterm. Only five women entered labor spontaneously, while seven (58,33%) had cesarean delivery.Conclusions: Pregnancy after liver transplantation can achieve relatively favorable outcomes. It does not influence women’s fertility and, during pregnancy, we report low rates of minor graft complications. A multidisciplinary team should be involved in contraceptive, fertility and consequently pregnancy counseling of female transplant recipients.
Alteration of Cortical Functional Networks in Default-Mode Network-Related Regions in Mood Disorders with Resting-State Electroencephalography
Authors: Kim Sungkean , Baek Ji Hyun , Shim Se-hoon , Kwon Young Joon , Lee Hwa Young , Yoo Jae Hyun , Kim Ji Sun 
Published: December 2020

Abstract
Studies comparing bipolar disorder (BD) and major depressive disorder (MDD) are scarce, and the neuropathology of these disorders is poorly understood. This study investigated source-level cortical functional networks using resting-state electroencephalography (EEG) in patients with BD and MDD. EEG was recorded in 35 patients with BD, 39 patients with MDD, and 42 healthy controls (HCs). Graph theory-based source-level weighted functional networks were assessed via strength, clustering coefficient (CC), and path length (PL) in six frequency bands. At the global level, patients with BD and MDD showed higher strength and CC, and lower PL in the high beta band, compared to HCs. At the nodal level, compared to HCs, patients with BD showed higher high beta band nodal CCs in the right precuneus, left isthmus cingulate, bilateral paracentral, and left superior frontal, belonging to the default-mode network (DMN); however, patients with MDD showed higher nodal CC only in the right precuneus compared to HCs. Although both MDD and BD patients had similar global level network changes, they had different nodal level network changes in DMN-related regions. Our findings might suggest more altered network in the DMN-related regions in patients with BD than in those with MDD.
Evaluation of the Foveal Avascular Zone in Familial Exudative Vitreoretinopathy Using Optical Coherence Tomography Angiography
Authors: Hasegawa Tetsuya , Hirato Misaki , Kobashi Chieko , Yamaguchi Aya , Takagi Rina , Tanaka Yoshiaki , Kaburaki Toshikatsu , Kakehashi Akihiro 
Published: December 2020

Abstract
Background: To evaluate the foveal avascular zone (FAZ) and retinal structure in familial exudative vitreoretinopathy (FEVR).Methods: Twenty FEVR eyes with stage 1 or 2 disease and 20 control eyes were evaluated. The central retinal thickness (CRT), inner retinal thickness (IRT), surface retinal vessel density (SRVD), and deep retinal vessel density (DRVD) were measured using optical coherence tomography. The FAZ area was calculated using ImageJ software. The equivalent spherical value (SE) and axial length (AL) were measured. Results: The CRT (232±26.75 vs. 213.15 ± 16.138 μm; p=0.0003) and IRT (17.44±13.28 vs. 1.85 ± 5.696 μm; p=0.0005) were thicker in the FEVR group than in the control group. The surface FAZ area (0.26±0.1 vs. 0.33 ± 0.1 mm2; p=0.006) and the deep FAZ area (0.36±0.1 vs. 0.43 ± 0.1 mm2; p=0.037) were smaller in the FEVR group than in the control group. The SRVD values did not differ among the sectors, but the DRVD was higher in the FEVR group except for the inferior sector (superior, p=0.02; inferior, p=0.4; temporal, p=0.001; nasal, p=0.02). The SE and AL did not differ between the two groups. There was no correlation between the surface and deep layer FAZ area and age, CRT, SE, and AL. The surface, deep FAZ area, and IRT were correlated negatively (surface, r = −0.57, p=0.008; deep layer FAZ area, r = −0.5, p=0.02).Conclusion: Eyes with FEVR has a smaller FAZ because the inner retina with the vascular structure remained in the fovea.
The Incidence, Risk Factors and Maternal and Foetal Outcomes of Uterine Rupture During Different Birth Policy Periods: An Observational Study in China
Authors: Zhou Yangwen , Mu Yi , Chen Peiran , Xie Yanxia , Zhu Jun , Liang Juan 
Published: December 2020

Abstract
Background: Currently, there are no studies on changes in the incidence of uterine rupture or maternal and foetal outcomes after uterine rupture during different birth policy periods in China. Moreover, the results of association studies of maternal age, parity and previous caesarean section number with the risk of maternal and foetal outcomes after uterine rupture have not been consistent. This research aims to conduct and discuss the above two aspects. Methods: We included singleton pregnant women with no maternal complications other than uterine rupture from January 2012 to June 2019 in China’s National Maternal Near Miss Surveillance System. Through Poisson regression analysis with a robust variance estimator, we compared the incidences of uterine rupture and maternal and foetal outcomes after uterine rupture during different birth policy periods in China and determined the relationship between maternal age, parity or previous caesarean section number and uterine rupture or maternal and foetal outcomes after uterine rupture. Results: This study included 8,637,723 pregnant women. The total incidences of uterine rupture were 0.13% (12,934) overall, 0.05% during the one-child policy, 0.12% during the partial two-child policy (aRR=1.96; 95% CI: 1.53-2.52) and 0.22% (aRR=2.89; 95% CI: 1.94 4.29) during the universal two-child policy. The maternal near miss and stillbirth rates after uterine rupture were respectively 2.35% (aRR=17.90; 95% CI: 11.81-27.13) and 2.12% (aRR=4.10; 95% CI: 3.19 5.26) overall, 5.46% and 8.18% during the first policy, 1.72% (aRR=0.60; 95% CI: 0.32-1.17) and 2.02% (aRR=0.57; 95% CI: 0.37-0.83) during the second policy, and 1.99% (aRR=0.90; 95% CI: 0.52-1.53) and 1.04% (aRR=0.36; 95% CI: 0.24-0.54) during the third policy. The risk of uterine rupture increased with parity and previous caesarean section number. Conclusion: Our findings suggest that the total and different characteristics risk of uterine rupture in China increased and the risk of stillbirth after uterine rupture improved from the one-child policy period to the universal two-child policy period. The risk of uterine rupture increased with parity and previous caesarean section number. The higher the parity and lower the previous caesarean section number was, the more serious the maternal and foetal outcomes would be.
Genetic Profiling in Heterogeneous High-Grade Adenoid Cystic Carcinoma Identifies NOTCH1 Inhibition as a Therapeutic Strategy
Authors: Wang Yu , Sun Bao , Zhang Chunye , Xia Ronghui , Wang Lizhen , Sun Jingjing , Gu Ting , Tian Zhen , Li Jiang 
Published: December 2020

Abstract
BackgroundHigh-grade adenoid cystic carcinomas (ACCs), consisting mostly of the solid type along with the occasional high-grade transformation (ACC-HGT) type, are highly aggressive and heterogeneous tumors. Because of the rarity of the tumors, therapeutic strategy guided by genetic profiles based on next generation sequencing (NGS) has been not published on these tumors. MethodsForty-four tissues microdissected from 36 high-grade ACC cases (31 solid type ACCs and 5 ACC-HGTs) were included in our study. The preclinical efficiency of targeted drugs were evaluated in ACC patient-derived xenografts (PDXs) models.ResultsThe solid components from the 31 solid ACCs, were enriched for mutations of genes in the NOTCH (NOTCH1, 68%; SPEN 23%) and the chromatin-remodeling pathway and were absent of the myoepithelial cell differentiation. The cases with NOTCH1 mutation exhibited strong NCID expression, which was associated with poor distant metastasis-free survival. BRCA2 mutation was observed in 23% of solid ACCs, conferring sensitivity to PARP inhibitors. From 5 of the solid ACCs, the intratumor heterogeneity was delineated between the cribriform/tubular and the solid components. NOTCH1 and FGFR2 mutations as well as NOTCH2 amplification were restricted to the solid component indicating the clonal selection within the same tumor. From 2 aggressive solid ACCs the subclones evolved in progression for local relapse and distant metastasis, although they manifested close genomic resemblance to primary tumors. Five tumors of ACC-HGTs were characterized by a loss of biphasic ductal-myoepithelial differentiation in HGT area and enriched for alterations in the NOTCH, P53 and PI3K pathways. Guided by the genetic profiles, the preclinical efficiency of a gamma-secretase inhibitor BMS-906024 demonstrated robust antitumor effects in the PDX models with activating NOTCH1 mutation. ConclusionscThis is the first report to describe the intratumor heterogeneity within a solid ACC as well as the intertumor evolution of clones among the primary/recurrent/metastatic tumors. Different from conventional ACCs, high-grade ACCs should be approached with a distinct therapeutic strategy particularly targeting NOTCH1 by gamma-secretase inhibitors. Microdissecting the highest grade component guided by histology is a highly recommended tumor sampling strategy and facilitates the detection of key molecular targets.
Chemotherapy Agents Stimulate Cytotoxic T Cell Against Human Colon cancer Cells through Upregulation of the Transporter Associated with Antigen Processing
Authors: Liang Yi-Hsin , Tsai Jia-Huei , Cheng Yung-Ming , Chan Kuang-Yu , Hsu Wen-Ling , Lee Chang-Cheng , Chen Kuo-Hsing , Yeh Kun-Huei 
Published: December 2020

Abstract
Objectives: Single immunotherapy fails to demonstrate efficacy in patients with microsatellite stable (MSS) metastatic colorectal cancer (mCRC). Research on immune reactions before and after systemic agents for mCRC is warranted. Methods: Our study examined cell line models to compare the expression of immune surface markers on colon cancer cells before and after chemotherapy agents. We also elucidated mechanisms underlying the effects of chemotherapy agents on immune surface markers. We used real-world clinical samples with NanoString analysis and the Perkin-Elmer Opal multiplex system.Results: We established that chemotherapy agents, particularly 7-ethyl-10-hydroxycamptothecin (SN-38), the active metabolite of irinotecan, stimulated the expression of stimulatory MHC class I isotypes through stimulation the pathway of transporters associated with antigen processing 1 and 2 (TAP1 and TAP2) in cell line models. Application of infected cell protein 47 (ICP-47), a specific inhibitor of the TAP1/TAP2, significantly inhibited expression of TAP1/TAP2 and also inhibited the expression of the downstream MHC class I. We confirmed that the expression of major histocompatibility complex (MHC) class I, programmed death 1(PD-1), and programmed death ligand 1(PD-L1) significantly increased after first-line chemotherapy and targeted therapy in the samples of real-world patients with de novo mCRC.Conclusion: Our study provides new insights for novel immunotherapy combinations.
Comparative Transcriptomic Analysis of Thermally Stressed Arabidopsis thaliana Meiotic Recombination Mutants
Authors: Huang Jiyue , Wang Hongkuan , Wang Yingxiang , Copenhaver Gregory 
Published: December 2020

Abstract
Background: Meiosis is a specialized cell division that underpins sexual reproduction in most eukaryotes. During meiosis, interhomolog meiotic recombination facilitates accurate chromosome segregation and generates genetic diversity by shuffling parental alleles in the gametes. The frequency of meiotic recombination in Arabidopsis has a U-shaped curve in response to environmental temperature, and is dependent on the Type I, crossover (CO) interference-sensitive pathway. The mechanisms that modulate recombination frequency in response to temperature are not yet known. Results: In this study, we compare the transcriptomes of thermally-stressed meiotic-stage anthers from msh4 and mus81 mutants that mediate the Type I and Type II meiotic recombination pathways, respectively. We show that heat stress reduces the number of expressed genes regardless of genotype. In addition, msh4 mutants have a distinct gene expression pattern compared to mus81 and wild type controls. Interestingly, ASY1, which encodes a HORMA domain protein that is a component of meiotic chromosome axes, is up-regulated in wild type and mus81 but not in msh4. We also characterize 51 novel, previously unannotated transcripts, and show that their promoter regions are associated with A-rich meiotic recombination hotspot motifs. Conclusions: Our transcriptomic analysis of msh4 and mus81 mutants enhances our understanding of how the Type I and Type II meiotic CO pathway respond to environmental temperature stress and might provide a strategy to manipulate recombination levels in plants.
Genome-Wide Association Study of Actinidia Chinensis
Authors: Zhang Yu , Wang Yuexing , Zheng Shimao , Zhou Wanying , Zhang Wenhui , Wang Shengbao , Qu Dong 
Published: December 2020

Abstract
Background: A total of 74,936 SNPs were employed to carry out GWAS and post-GWAS of the fifty-six accessions, representing the two most valuable varieties of Actinidia chinensis, namely, A. chinensis var. chinensis and A. chinensis var. deliciosa, are mainly distributed in China. The percentage of heterozygous sites of A. chinensis var. deliciosa is higher than that of A. chinensis var. chinensis, which could be one of the reasons for A. chinensis var. deliciosa high disease resistance. LD decay distance of male plants is shorter than that of female plants. Results: Fifty-six accessions were divided into two subgroups. Analysis of molecular variance shows that the frequency of genetic variations within the population is 83.53% and 16.47% between populations. Fst between the two populations is 0.14, and Nm is 1.60. Set at α ≤ 0.05, a total of 327 SNPs and 260 haplotypes were related to the hispidus. A total of 246 proteins were annotated using GO and KEGG analyses, which indicated the membrane-related genes and stress-resistant metabolic pathways are related to the hispidus condition of leaves, stems, and peels of kiwifruit, which is also the result of the adaptation of A. chinensis var. deliciosa to its growth environment. Conclusions: Haplotype analysis showed that the evolution of is A. chinensis var. deliciosa later than that of A. chinensis var. chinensis.
Rare and Potentially Pathogenic Variants in Hydroxycarboxylic Acid Receptor Genes Identified in Breast Cancer Cases.
Authors: Sams Cierla McGuire , Shepp Kasey , Pugh Jada , Bishop Madison R. , Merner Nancy D. 
Published: December 2020

Abstract
Background: Three genes, clustered together on chromosome 12, comprise a group of Hydroxycarboxylic Acid Receptors (HCARs), HCAR1, HCAR2, and HCAR3. These paralogous genes encode different G-protein coupled receptors that are responsible for detecting glycolytic metabolites and controlling fatty acid oxidation. Though better known for regulating lipid metabolism in adipocytes, more recently HCARs have been functionally associated with breast cancer proliferation/survival; HCAR2 has been described as a tumor suppressor, and HCAR1 and HCAR3 as oncogenes. Thus, we sought to identify genetic variants in HCAR1, HCAR2, and HCAR3 that could potentially be associated with breast cancer. Methods: Due to the extreme homology between HCAR1, HCAR2, and HCAR3, primers were carefully designed to amplify each gene separately through nested PCRs followed by Sanger sequencing. Forty-six unrelated breast cancer cases were screened for rare, non-synonymous coding variants. Results: Upon screening, a total of four variants were identified in four different cases, each with estrogen receptor-positive (ER+) breast cancer. The variants were identified exclusively in HCAR1 and HCAR3. In HCAR1, two highly conserved and potentially damaging missense variants were identified, c.58C>G;p.Pro20Ala and c.721C>T;p.Leu241Phe. Statistical analyses revealed that c.58C>G;p.Pro20Ala was in significantly more cases than controls. In HCAR3, in addition to the breast cancer-associated missense variant c.560G>A;p.Arg187Gln, a frameshift mutation, c.1117delC;p.Gln373Lysfs*82, was detected that greatly extends the C-terminus and changes the secondary and tertiary protein structure. Conclusions: Due to the important role of HCARs in breast cancer, it is vital to understand how these genetic variants play a role in breast cancer risk and proliferation, as well as their consequences on treatment strategies. Due to the small sample size, additional and larger studies will be needed to validate these findings. Nevertheless, the identification of these potentially pathogenic variants supports the need to investigate their functional consequences.
Identification of Hub Genes Associated With Clinical Characteristics and Potential Therapy of Diabetic Nephropathy by Bioinformatics Analysis.
Authors: Xu Si , Li Xiaoning , Wu Sha , Yang Min 
Published: December 2020

Abstract
Background: To provide theoretical basis for the molecular mechanism of the development of diabetic nephropathy and targeted molecular therapy by screening expressed genes based on bioinformatic analysis. Methods: We analyzed diabetic nephropathy microarray datasets derived from GEO database. Perl and R programming packages were used for data processing and analysis and for drawing. STRING online database and Cytoscape software were utilized for protein-protein interaction network analysis and screened for hub genes. Also, WebGestalt was used to analyze the relationship between genes and microRNAs. Nephroseq online tool was used to visualize the correlation between genes and clinical properties.Results: We found 91 differentially expressed genes between diabetic nephropathy tissues and normal control tissues. Protein-protein interaction network analysis screened out 5 key modules and a total of 14 hub genes were identified by integration, also11 microRNAs were associated with hub genes. Especially mir29 could regulate COL6A3 and COL15A1.Conclusions: The internal biological information in diabetic nephropathy can be revealed by integrative bioinformatical analysis, providing theoretical basis for further research on molecular mechanism and potential targets for diagnosis and therapeutics of diabetic nephropathy.