bluuebunny/crossref_metadata_2025_split huggingface dataset:
A biochemical comparison of the lung, colonic, brain, renal, and ovarian cancer cell lines using 1H-NMR spectroscopy
Authors: Hu Cong , Liu Zhigang , Zhao Hailin , Wu Lingzhi , Lian Qingquan , Ma Daqing , Li Jia V. 
Published: April 2020

Abstract
Cancer cell lines are often used for cancer research. However, continuous genetic instability-induced heterogeneity of cell lines can hinder the reproducibility of cancer research. Molecular profiling approaches including transcriptomics, chromatin modification profiling, and proteomics are used to evaluate the phenotypic characteristics of cell lines. However, these do not reflect the metabolic function at the molecular level. Metabolic phenotyping is a powerful tool to profile the biochemical composition of cell lines. In the present study, 1H-NMR spectroscopy-based metabolic phenotyping was used to detect metabolic differences among five cancer cell lines, namely, lung (A549), colonic (Caco2), brain (H4), renal (RCC), and ovarian (SKOV3) cancer cells. The concentrations of choline, creatine, lactate, alanine, fumarate and succinate varied remarkably among different cell types. The significantly higher intracellular concentrations of glutathione, myo-inositol, and phosphocholine were found in the SKOV3 cell line relative to other cell lines. The concentration of glutamate was higher in both SKOV3 and RCC cells compared with other cell lines. For cell culture media analysis, isopropanol was found to be the highest in RCC media, followed by A549 and SKOV3 media, while acetone was the highest in A549, followed by RCC and SKOV3. These results demonstrated that 1H-NMR-based metabolic phenotyping approach allows us to characterize specific metabolic signatures of cancer cell lines and provides phenotypical information of cellular metabolism.
m6A-dependent up-regulation of DRG1 by METTL3 and ELAVL1 promotes growth, migration, and colony formation in osteosarcoma
Authors: Ling Zhian , Chen Liangjun , Zhao Jinmin 
Published: April 2020

Abstract
Osteosarcoma (OS) is a malignant tumor commonly observed in children and adolescents. Developmentally regulated GTP-binding protein (DRG) 1 plays an important role in embryonic development; aberrantly expressed DRG1 has been associated with pathological processes in cancer. The present study aimed to explore the role of DRG1 in OS and the mechanisms underlying DRG1 overexpression in OS. Clinical studies were performed to evaluate Drg1 expression in OS tissues and to identify a correlation between Drg1 expression and the clinicopathological features in patients with OS. Drg1 was knocked down in OS cells to determine its effects on cell viability, cell cycle distribution, apoptosis, migration, invasion, and colony formation rate. METTL3 and ELAVL1 were also silenced to determine their effects on the levels of N6-methyladenosine (m6A), RNA stability, and Drg1 expression. Higher levels of Drg1 mRNA and protein were observed in OS tissues than those in paracancerous tissues. High expression of DRG1 was associated with large tumor size and advanced clinical stages in OS. Silencing of Drg1 resulted in decreased viability and inhibition of the migration and colony formation abilities of OS cells; it also resulted in cell cycle arrest in the G2/M stage and induced apoptosis. Knockdown of METTL3 led to decreased m6A and Drg1 mRNA levels. ELAVL1 knockdown impaired the stability of DRG1 mRNA, thereby reducing both the mRNA and protein levels of DRG1. In all, DRG1 exerted tumorigenic effects in OS, and the up-regulation of DRG1 in OS was induced by METTL3 and ELAVL1 in an m6A-dependent manner.
Dual‐output unity power factor rectifier power block
Authors: Rana Mandeep Singh , Mishra Santanu K. 
Published: April 2020

Unity power factor rectifiers find applications in modern utility systems and consumer electronic devices. However, a dedicated rectifier is required for a given load. In this study, a Unity Power Factor (UFP) power block is described which can generate two DC outputs, one buck and one boost, with a reduced number of switches and associated driver circuits. The topology adapts well with conventional control as it retains the dynamic behaviour of a buck and boost topology. While supplying simultaneous loads it exhibits minimal cross‐regulation interference between the outputs. The controller implementation for the proposed power block is described and its difference with the conventional Power Factor Correction (PFC) controller is outlined. The topology along with its control to regulate the outputs and force UPF operation are validated using a 200 W prototype with 110 V utility input and 180–24 V DC outputs.
X-ray andγ-ray orbital variability from theγ-ray binary HESS J1832−093
Authors: Martí-Devesa G. , Reimer O. 
Published: April 2020

Context.γ-ray binaries are systems composed of a massive star and a compact object whose interaction leads to particle acceleration up to relativistic energies. In the last fifteen years, a few binaries have been found to emit at high energies, but their number is still low. The TeV source HESS J1832−093 has been proposed as a binary candidate, although its nature is unclear. Neither a GeV counterpart nor a period was detected.Aims.The purpose of this work is to search for a GeV counterpart to understand the origin of the TeV signal detected by H.E.S.S. For an unambiguous identification of its binary nature, finding an orbital modulation is crucial.Methods.We analysed data spanning more than 10 years from theFermiLarge Area Telescope (Fermi-LAT), together withSwiftarchival observations taken between 2015 and 2018, using both the X-Ray Telescope and UV/Optical Telescope. We searched for periodicities in both X-ray and GeV bands.Results.We find a periodic modulation of ∼ 86 days in the X-ray source candidate counterpart XMMU J183245−0921539, together with indications ofγ-ray modulation with a compatible period in the GeV candidate counterpart 4FGL J1832.9−0913. Neither an optical nor a UV counterpart is found at the X-ray source location. The overall spectral energy distribution strongly resembles the knownγ-ray binary HESS J0632+057.Conclusions.Both the spectral energy distribution and the discovery of an orbital period allow the identification of the TeV source HESS J1832−093 as a new member of theγ-ray binary class.
Systemische Therapieoptionen bei lokal fortgeschrittenen und metastasierten myxoiden, dedifferenzierten und pleomorphen Liposarkomen
Authors: Pink Daniel , West Antje , Andreou Dimosthenis 
Published: April 2020

ZusammenfassungNoch vor wenigen Jahren haben alle Patienten mit metastasierten Weichteilsarkomen die gleiche Chemotherapie erhalten. Infolge der Erkenntnis, dass es sich bei den verschiedenen Sarkomsubtypen um unterschiedliche Erkrankungen mit unterschiedlichen genetischen Veränderungen und einem unterschiedlichen biologischen Verhalten handelt, wurden allerdings in letzter Zeit zunehmend subtypspezifische medikamentöse Therapieprotokolle eingesetzt. Selbiges gilt auch für die verschiedenen Subtypen von Liposarkomen – das myxoide/rundzellige Liposarkom, das dedifferenzierte Liposarkom und das pleomorphe Liposarkom. In diesem Beitrag werden publizierte Daten und Erfahrungen mit unterschiedlichen systemischen Therapieoptionen in den verschiedenen Therapielinien sowie ein Überblick über experimentelle Therapieansätze präsentiert.
Design and Usability of an Electronic Health Record—Integrated, Point-of-Care, Clinical Decision Support Tool for Modeling and Simulation of Antihemophilic Factors
Authors: Abdel-Rahman Susan M. , Gill Harpreet , Carpenter Shannon L. , Gueye Pathe , Wicklund Brian , Breitkreutz Matt , Ghosh Arindam , Kollu Avinash 
Published: April 2020

Abstract
Background With the consequences of inadequate dosing ranging from increased bleeding risk to excessive drug costs and undesirable administration regimens, the antihemophilic factors are uniquely suited to dose individualization. However, existing options for individualization are limited and exist outside the flow of care. We developed clinical decision support (CDS) software that is integrated with our electronic health record (EHR) and designed to streamline the process for our hematology providers.
Objectives The aim of this study is to develop and examine the usability of a CDS tool for antihemophilic factor dose individualization.
Methods Our development strategy was based on the features associated with successful CDS tools and driven by a formal requirements analysis. The back-end code was based on algorithms developed for manual individualization and unit tested with 23,000 simulated patient profiles created from the range of patient-derived pharmacokinetic parameter estimates defined in children and adults. A 296-item heuristic checklist was used to guide design of the front-end user interface. Content experts and end-users were recruited to participate in traditional usability testing under an institutional review board approved protocol.
Results CDS software was developed to systematically walk the point-of-care clinician through dose individualization after seamlessly importing the requisite patient data from the EHR. Classical and population pharmacokinetic approaches were incorporated with clearly displayed estimates of reliability and uncertainty. Users can perform simulations for prophylaxis and acute bleeds by providing two of four therapeutic targets. Testers were highly satisfied with our CDS and quickly became proficient with the tool.
Conclusion With early and broad stakeholder engagement, we developed a CDS tool for hematology provider that affords seamless transition from patient assessment, to pharmacokinetic modeling and simulation, and subsequent dose selection.
A Pilot Study of a Novel Fixation Technique for Fixation of Comminuted Patellar Fractures: Arthroscopic-Controlled Reduction and Circular External Fixation
Authors: Neyisci Cagri , Erdem Yusuf , Kilic Erden , Arsenishvili Arsen , Kürklü Mustafa 
Published: April 2020

AbstractPatella fractures represent for 0.5 to 1.5% of all bony injuries in adults. Open reduction and the modified tension-band technique is the most common surgical technique used for patellar fractures. The purpose of this study is to present the outcomes of 26 comminuted patellar fractures treated with circular external fixator (CEF) under arthroscopic control and discuss its potential advantages over conventional surgical methods. This retrospective study included 26 patients who had closed comminuted patellar fractures and treated by CEF under arthroscopic control between January 2002 and March 2016. All patients treated with this technique were involved to the study as a consecutive series. Patients with noncomminuted transverse fractures were excluded, because they were treated with a different technique. Of the 26 patients 22 were male, 4 were female with the mean age of 33.5 years (range, 16–56 years). Patients were followed for 20 to 28 months (mean, 22 months). The mean time to union and the duration of fixation with the CEF ring was 12 weeks (range, 6–15 weeks). The mean Lysholm's score was 45 (range, 35–58) at the 10th postoperative day, which increased to 51 (range, 40–68) at the end of the first postoperative month and increased to 95 (range, 90–100) 1 month after CEF ring removal. Minor pin tract infection by pin-skin irritation was observed in nine patients. In one patient, refracture occurred due to a fall 19 days after CEF removal. CEF appears to be a safe and effective treatment for comminuted patellar fractures with a high union rate and minimal complications. It is safe and effective, as it allows short hospital stay and avoids a second surgery for removal of the instrument. Early rehabilitation with full weight-bearing promotes rapid recovery and quick return to work. Patients do not have a large unaesthetic scar on the anterior of the knee.
Closed Incisional Negative Pressure Wound Therapy at Flap Suture Line: An Innovative Approach for Improving Outcomes in Suboptimal Wound Conditions
Authors: Naalla Ravikiran , Bhushan Smriti , Abedin Minhaj Ul , Bichpuriya Ashish Dhanraj , Singhal Maneesh 
Published: April 2020

Abstract
Background Persistent dead space following flap cover is a frequently encountered challenge following the reconstruction of complex wounds. It may lead to a hematoma, seroma, wound infection, and wound dehiscence. Wound dehiscence could be a devastating complication. Closed incisional negative pressure wound therapy (ciNPWT) over the surgical incisions was found to reduce surgical site infection (SSI) and wound dehiscence. We applied this principle at the closed flap suture line and through this article, we share the indications, technique, and outcomes.
Methods A retrospective analysis (January 2018–June 2019), in which selected high-risk patients who underwent ciNPWT at the flap suture following complex reconstruction (pedicled or free flap) were included in the study. The indications include deep incisional/organ SSI after debridement and flap coverage, persistent dead space following flap coverage, chronic osteomyelitis. Patients were analyzed in the follow-up period in terms of complications, wound healing.
Results Nine patients underwent ciNPWT over the flap suture line. The mean age was 32.2 years (range: 10–48 years). The mean duration of the NPWT application was 7.3 days (range: 3–21 days). Three of the nine patients had flap-related minor complications. One patient had marginal flap necrosis and required skin grafting, one patient had minor wound dehiscence (1 cm) which required secondary skin suturing and one patient had chronic discharging sinus related to osteomyelitis of ischium, which subsequently healed with antibiotics and local wound care. None of the patients had NPWT-related complications.
Conclusion Closed incisional NPWT decreases the untoward effects of dead space following the reconstruction of complex wounds. The incidence of SSI and wound gaping can be reduced.
Seasonal prediction of Euro-Atlantic teleconnections from multiple systems
Authors: Lledó Llorenç , Cionni Irene , Torralba Verónica , Bretonnière Pierre-Antoine , Samsó Margarida 
Published: April 2020

AbstractSeasonal mean atmospheric circulation in Europe can vary substantially from year to year. This diversity of conditions impacts many socioeconomic sectors. Teleconnection indices can be used to characterize this seasonal variability, while seasonal forecasts of those indices offer the opportunity to take adaptation actions a few months in advance. For instance, the North Atlantic Oscillation has proven useful as a proxy for atmospheric effects in several sectors, and dynamical forecasts of its evolution in winter have been shown skillful. However the NAO only characterizes part of this seasonal circulation anomalies, and other teleconnections such as the East Atlantic, the East Atlantic Western Russia or the Scandinavian Pattern also play an important role in shaping atmospheric conditions in the continent throughout the year. This paper explores the quality of seasonal forecasts of these four teleconnection indices for the four seasons of the year, derived from five different seasonal prediction systems. We find that several teleconnection indices can be skillfully predicted in advance in winter, spring and summer. We also show that there is no single prediction system that performs better than the others for all seasons and teleconnections, and that a multi-system approach produces results that are as good as the best of the systems.
Pharmacology potency of thin layer chromatography steroid isolates of Chlorella sp. chloroform fraction
Authors: Megawati D S , Fasya A G , Pratiwi R A , Maghfiroh N 
Published: April 2020

Abstract
Al-Qur’an surah as Syu’ara verse 7 shows that Allah SWT grew many good and advantages plants, such as microalgae Chlorella sp. Chlorella sp. are contained some secondary metabolites, such as steroid compounds. Purpose of this research is to know toxicity levels and antioxidant activity steroid compound of chloroform and ethyl acetate fractions from hydrolyzed methanol extract of microalgae Chlorella sp. Cultivation is conducted on a laboratory scale using Sprouts Extract Medium (SEM) 4% and harvesting conducted every 10th day. Dry Chlorella sp. biomass was macerated using methanol solvent. The extract then hydrolyzed with 2 N of HCl and partitioned with chloroform, then the separation using Thin Layer Chromatography (TLC). Isolates conducted toxicity tests using Brine Shrimp Lethality Test (BSLT) and antioxidant activity test with the DPPH method. The toxicity test showed that TLC steroid isolates of chloroform fraction had LC50 values 8.796 (C1), 8.53 (C4) and 4.53 ppm (C5). The antioxidants test showed that TLC steroid isolates of chloroform fraction had had a value of EC50 is 2.26 1019 (C1) 163.10 ppm (C4) and 38.96 ppm (C5). Identification of steroid compounds with UV-VIS spectrophotometer has a maximum wavelength of 250.50, 270.5 and 281 nm. Identification with FTIR showed functional groups C=O (carbonyl), C-O (ether), -C(CH3)2 (geminal dimethyl), C=C and = CH (alkenes) attributed to steroid compounds.
The effect of synthesis time to particle size of Bi4Ti3O12 which synthesized using molten single salt NaCl method
Authors: Januari T , Aini N , Barroroh H , Prasetyo A 
Published: April 2020

Abstract
Bi4Ti3O12 was synthesized by the molten salt method using single salt NaCl with calcination time variations: 2, 4, 8, and 16 hours at 900 °C. The effect of time synthesis on particle size and morphology was investigated. The X-ray diffraction data showed that the sample Bi4Ti3O12 was successfully synthesis and there is not found any impurities. The image from scanning electron microscopy showed that the shape of Bi4Ti3O12 particle is plate-like and particle size increases as calcination time.
Digital workplace: digital transformation for environmental sustainability
Authors: Yalina N , Rozas I S 
Published: April 2020

Abstract
Environmental Sustainability becomes a major issue around the world. Energy consumption and air pollution are some important issues that have been discussed among scholars and practitioners. The purpose of this paper is to examine how digital workplace implementation could contribute to empower environmental sustainability, why this is important and how this can be achieved. We collected the data from UIN Sunan Ampel Surabaya and do the simulation if their employees implement the digital workplace for three cases, 50% of the farthest employees, 50% of the nearest employees, and 50% on random. The efficiency of fuel used obtain was 44.39%, 5,61%, and 22.2% respectively. From the simulation, it can be concluded that the application of the digital workplace can be an alternative in the framework of global environmental sustainability.
New compounds of pregnanone from Calotropis gigantea roots actively against colon cancer cell WiDr through cell cycle inhibition
Authors: Mutiah R , Widyawaruyanti A , Sukardiman  
Published: April 2020

Abstract

Calotropis gigantea (L.) W. Aiton (C. gigantea) is a medicinal plant that has been empirically proven to have anticancer activity. In a previous study, it showed that the fraction of ethyl acetate from the root part of C. gigantea had higher anticancer activity than the other fractions. It suspected that the ethyl acetate fraction of C. gigantea root contained active compounds that has anticancer properties. This study aimed to determine the anticancer activity of active compounds from the ethyl acetate fraction of C. gigantea root regarding induction of apoptosis, cell cycle arrest, and expression of caspase-8 colon cancer cell WiDr. Isolation of the active compounds from the ethyl acetate fraction of C. gigantea root was carried out using Bioassay-guided Isolation method. Identification of active compounds was using NMR-1H, NMR-13C, HMBC, HMQC and UPLCMS/MS methods. The anticancer activity test of the identified compounds performed by using MTT method. The induction of apoptotic and cell cycle arrest evaluated by a flow cytometry method. The result of this study showed two active compounds were identified namely (1) (Pregnanon-5-en, 3,14,17 trihydroxy-12- (4’-cyclohexyl benzoate) -, (3β, 12β, 14β) - (9CI), (2) Pregn-5-en-20-one, 3,8,14-trihydroxy-12 - [(4’-hydroxy benzoyl) oxy] -, (3β, 12β, 14β, 17α) - (9CI). Both compounds inhibited the growth of colon cancer cell WiDr with IC50 values respectively were 15.89 μg/mL and 0.77 μg/mL. Both compounds increased the induction of apoptotic by increasing sub-G1, S, and G2-M following depletion of G0-G1 phase accumulation.
Disinfecting technology of Camellia sinensis L inoculants through in vitro culture
Authors: Sutini  , Widiwurjani  , Augustien N , Pribadi DU , Djoko A P 
Published: April 2020

Abstract
Leaves of the Camellia sinensis plant is a very economically valuable commodity and can be applied to various fields. Such as in the fields of agriculture, food and beverage and medicine. The problem that is found in the land area is the presence of plants that are more than tens of years old that need to be plant rejuvenated. Rejuvenation with grafting and cutting techniques is strongly influenced by the climate and extensive agricultural land. To overcome this problem, in vitro culture techniques are applied as an effort to conserving/producing plant seeds that are free of disease pests, controlled environments and with narrow land or laboratory scale. This research aimed to determine the inoculant of Camellia sinensis which gave the best results on the disinfecting through in vitro culture technique. The method used in the research was using two types of inoculants, they are shoot buds and young shoots in the disinfecting process, and each process was repeated fifteen times. The results obtained were that the disinfecting of Camellia sinensis plants with shoot bud inoculants showed the lowest level of contamination with the number of growing inoculants close to 85 percent in twelve weeks of inoculant harvesting.
Release test of N, P, and K of complete slow release fertilizer (PUKAP JESTRO-1) and its effect on the growth of young siam citrus (Citrus nobilis Lour.)
Authors: Sutopo  , Aji T G , Budiyati E 
Published: April 2020

AbstractThe research aimed to study the release of N, P, K elements from slow release complete fertilizer (PUKAP JESTRO-1) and its effect on the growth of Siam citrus in a high density planting system (HDP). The release test of PUKAP JESTRO-1 was carried out in a laboratory, where the remaining N, P, K content in fertilizer was measured periodically. The influence test of PUKAP JESTRO-1, which compared with conventional fertilizer in the form of a single fertilizer and NPK compound fertilizer, was carried out on a 1-month old Siam citrus planted on Grumusol (vertisol). The study used randomized block design with 5 treatments and 3 replications, namely 400 g/plant/year mixed fertilizer (200 g Urea + 150 g SP36 + 50 g KCl); 200 g/plant/year PUKAP JESTRO-1; 300 g/plant/year PUKAP JESTRO-1; 400 g/plant/year PUKAP JESTRO-1; 400 g/plant/year NPK YaraMila (16-16-16). The experimental results showed that PUKAP JESTRO-1 released N, P, and K until the fourth, tenth, and eighth week respectively. Application of PUKAP JESTRO-1 at 25% lower dosages produced better plant height compared to mixed fertilizer applications but did not significantly increase leaf N, P, and K levels due to the effect of dilution of elements in plant tissue.