bluuebunny/crossref_metadata_2025_split huggingface dataset:
Agrobacterium-mediated transformation of potato with human lactoferrin gene
Authors: Buziashvili A. Yu. , Yemets A. I. 
Published: November 2019

Aim. Obtaining of the new Ukrainian potato cultivars with complex resistance to phytopathogens. Methods. Agrobacterium-mediated transformation of potato cultivars Vernisage and Zarevo with human lactoferrin gene possessing antimicrobial activity was carried out. For transformation, supervirulent A. tumefaciens strain EHA 105 with hLf gene and gene for the resistance to kanamycine was used. Selection was carried out for 3 months on MSK-S1 and MSK-S2 media supplemented with 100 mg/l kanamycine. Integration of lactoferrin gene in transgenic plant lines was confirmed by polymerase chain reaction with specific for hLf gene primers. Results. After selection, potato lines with the resistance to kanamycine were obtained. The transgenic potato lines with integrated into their genomes hLf gene were obtained. Integration of lactoferrin gene was confirmed for 1 line of Zarevo cultivar and 3 lines of Vernisage cultivar. Transformation frequency was of 6.8% for cv. Vernisage and 6.25% for cv. Zarevo. Conclusions. Selected potato lines would be used in further studies on their resistance to bacterial and fungal pathogens.
Keywords: human lactoferrin gene hLf, Solanum tuberosum, Agrobacterium-mediated transformation, transgenic plants.
Establishment of glyphosate selective concentrations for winter rapeseed (Brassica napus L.) transgenic tissues efficient selection in vitro
Authors: Hnatiuk I. S. , Varchenko O. I. , Parii M. F. , Symonenko Yu. V. 
Published: November 2019

Aim. To establish the optimal concentration of glyphosate for efficient selection of transgenic tissue culture in biotechnology of rapeseed by in vitro selection of glyphosate-resistant and non-resistant winter rape. Methods. As an explants 7–10 mm fragments of 6-day rapeseed hypocotyls were used to cultivate on the medium, supplemented with 1 mg/L of 2,4-dichlorophenoxyacetic acid (2,4-D) for 12 days at 24°C under dark conditions for the initiation of callusogenesis. For regeneration of plants, the MS nutrient medium was supplemented with 3 mg/L of 6-benzylaminopurine (BAP), 2 mg/L of zeatin, 0.1, 0.5 and 1 mM of glyphosate, respectively. As a control, glyphosate-free regenerative nutrient medium was used. The results were statistically processed using Microsoft Excel. Results. It has been shown that glyphosate-susceptible rape explants form leaves and shoots with a frequency up to 8.3 % ± 0.8 % on the medium supplemented with 0.1 mM glyphosate compared to 57.2 % ± 0.6 % in the control. However, the morphogenic structures did not pass selection after transferring shoots to the elongation medium supplemented with 0.1 mM glyphosate. Conclusions. For the in vitro selection of rapeseed 0.1 mM glyphosate can be used in a nutrient medium at the stage of adventitious buds formation. But at the regeneration stage, the amount of herbicide should be increased to avoid false-positive results.
Keywords: winter rapeseed, Brassica napus L., in vitro culture, selective marker, herbicide, glyphosate.
Role of chelated fertilizers` and nano-particles application for formation of elements of productivity аt artificial infected with phytoplasmas and virus
Authors: Huliaieva H. B. , Bohdan M. M. , Tokovenko I. P. , Patyka V. P. 
Published: November 2019

Aim. Investigation of the role of chelated fertilizer and nanoparticles in the formation of elements of productivity for artificial infection of phytoplasmas and wheat streak mosaic virus. Methods. Microbiological, serological, biophysical, biometric, statistical. Results. In the field conditions, the effect of leaf treatment of wheat plants with chelated fertilizers and nanoparticles of copper and silver for artificial infection of phytoplasmas and WSMV on the magnitude of the quantum efficiency of photosystem II for controlling replication of the virus by the method of immunoassay analysis and the study of the influence of nanoparticles on the development of phytoplasmas, as well as the weight of 1000 grains of wheat was investigated. Conclusions. Foliar treatment with chelated fertilizer can activate the plant’s phytoimmunity of wheat plants, thus preventing the active replication of the virus in the plant organism, thereby reducing somewhat the loss of productivity for the damage to the WSMV. The use of foliar treatment of wheat plants with 1% solution nanoparticles of silver and copper citrates can stop the development of phytoplasma infection, which allows maintaining a level of productivity close to the intact plants.
Keywords: Triticum aestivum L., WSMV, phytoplasma, chlorophyll a fluorescence induction, productivity.
Adaptive reactions of corn plants to acute ultraviolet radiation
Authors: Zhuk V. V. , Mikheyev A. N. , Ovsyannikova L. G. 
Published: November 2019

Aim. The goal of this work was to study the adaptive reaction of young corn plants (Zea mays L.) to the action of acute ultraviolet B (UV-B) radiation in the vegetative growth period. Methods. Corn plants hybrid Dostatok 300 MV in the phase of two leaves were irradiated by UV-B at doses of 1, 2, 4, 8, 16 kJ/m2 with a power of 6 W/m2 and were grown in a long day conditions. After irradiation, the growth of shoots in length, the growth of the mass of plants, the content of photosynthetic pigments and endogenous hydrogen peroxide (HP) in the leaves were determined. Results. It is shown that after irradiation of corn plants UV-B at doses of 4, 8, 16 kJ/m2 the growth of shoots in length decreased, after the irradiation of UV-B at doses of 1, 2 kJ/m2 mass of plants increased. UV-B irradiation in all doses, except for a dose of 16 kJ/m2, stimulated the accumulation of photosynthetic pigments in the leaves. Increasing HP content in the leaves was determined in corn leaves after irradiation of UV-B in all doses. Conclusions. It is shown that irradiation of young corn plants by acute UV-B at doses from 1 to 16 kJ/m2 induced adaptive reactions, that included inhibition of shoot growth with simultaneous stimulation of plants mass growth, accumulation of photosynthetic pigments, increased endogenous content of HP. The adaptive period lasted about 10 days, after that processes of growth, formation of a photosynthetic apparatus and content of HP were stabilized. UV-B belongs to the necessary components of sunlight components for corn growth. Keywords: UV-B radiation, Zea mays L., photosynthetic pigments, adaptation.
Callus induction from shoot apical meristem in Triticum spelta L. and T. aestivum L.
Authors: Kyriienko A. V. , Parii M. F. , Symonenko Yu. V. , Kuchuk M. V. , Shcherbak N. L. 
Published: November 2019

Aim. To develop an effective protocol for callus induction from shoot apical meristem in Triticum spelta L. and T. aestivum L. Methods. Plant material: spelt “Europe” and common wheat “Bunchuk”. For this research we used shoot apical meristems from 3-days plants. For callus induction we proposed 4 media with different concentration of 2,4-D, picloram, NAA and AgNO3. Explants were growing in dark during 21 day at + 25 C. Results. Calli were transparent and mild, less than 8 mm. For callus induction positive effect were shown on media with 2,4-D and picloram. At the same time, NAA was not such effective. Conclusions. In our research was shown, that the best media for spelt callus induction should have 2 mg/l 2,4-D and 10 mg/l AgNO3.
Keywords: callusogenesis, spelt (Triticum spelta L.), common wheat, callus, shoot apical meristem.
Extraction of biologically active compounds from the residues of pericarps of sugar beet (Beta vulgaris L. ssp. vulgaris)
Authors: Klyachenko O. L. , Janse L. A. , Likhanov A. F. 
Published: November 2019

Aim. In the regulation of germination of seeds and of growth and development of seedlings, an important role belongs to biologically active substances that are found in the root of sugar beet (Beta vulgaris L. ssp. vulgaris), mainly in the pericarp. Extraction of secondary metabolites and study of the influence of a multicomponent system of endometabolites (phenolic compounds) of sugar beet pericarps on germination of plant seeds. Methods. The chemical composition of the metabolites of fruits and pericarps of sugar beet was determined by thin-layer chromatography; their differentiated isolation was carried out using column chromatography and gel filtration; The total content of phenolic compounds in fruits and pericarps was determined spectrophotometrically. Results. It was found that sugar beet pericarps contain a considerable amount of phenolic compounds (5-10 %). Four groups of phenolic compounds with different molecular masses were detected. By studying the influence of aquatic extracts on radish seeds, it was found that the eluents of the first volumes of water (3-5 ml) showed a pronounced stimulating effect, whereas further volumes of eluents (8-9 ml) were characterized by inhibitory action. Conclusions. Phenols, flavonoids and their glycosides were detected in extracts of sugar beet pericarps, which can be characterized as highly active inhibitors and growth promoters, therefore they could be used as natural biologically active substances.
Keywords: sugar beet, pericarp, biologically active compounds, phenols, flavonoids.
The creating of intergenetic hybrids of festulolium of Festuca arundinacea morphotipe with the use of post-genomic technologies and DNA-marking
Authors: Kondratskaya I. P. , Yukhimuk A. N. , Stolepchenko V. A. , Chizhik O. V. , Kozlovskaya Z. G. , Vasko P. P. , Reshetnikov V. N. 
Published: November 2019

Aim. To form the varietal population of festulolium intergeneric hybrids of Festuca arundinacea morphotype. To carry out DNA-labeling of created festulolium hybrid plants and parental forms. Methods. The festulolium intergeneric hybrid’s creation was carried out by embryo culture method from an immature caryopsis by growing on a nutrient medium. For the plant genotypes labeling the multilocus primers associated with coding DNA regions (Start Codon Targeted (SCoT) Polymorphism), SRAP (Sequence-related amplified polymorphism) have been selected. Results. The viable plants of intergeneric festulolium hybrids of Festuca arundinacea morphotype have been obtained. To select the best festulolium biotypes for variety populations with high feed and seed productivity formation. A system for hybrid plants genotypes and their parental forms registration in the form of molecular genetic passports have been elaborated. The genetic passports reflects the allele’s composition in loci associated with DNA coding sequences. Conclusions. The best biotypes with economically valuable traits were selected and included in the further selection process. The molecular genetic passports of hybrid festulolium plants and parental forms were composed.
Keywords: festulolium, immature caryopsis, biotypes, DNA, PCR, molecular genetic passports.
Evaluation of some physiological and biochemical parameters of Camelina sativa seedlings under osmotic stress
Authors: Nishchenko L. V. , Sakhno L. O. 
Published: November 2019

Aim. To find the most drought resistant spring camelina (Camelina sativa (L.) Crantz) genotype for further involvement in genetic transformation experiments, the some physiological and biochemical parameters of seedlings have been investigated under normal conditions and osmotic stress in vitro. Methods. Sterile seeds were planted on Murashige and Skoog agarized medium without hormones or the same media supplemented with mannitol as an osmotic stress inductor. Germination, fresh weight, total soluble protein content, and superoxide dismutase (SOD) activity have been investigated in 7-day-old seedlings. Results. The analyzed camelina genotypes were differed in their reactions on water deficit in vitro. It has been revealed that higher SOD activity of FEORZhYaF-1 seedlings was accompanied by higher germination and higher ability to fresh weight accumulation under osmotic stress as compared to other varieties. Conclusions. FEORZhYaF-1 breeding form could be involved in genetic transformation experiments to improve drought resistance. SOD activity should be used as a parameter for selection of osmotic stress resistant plant material.
Keywords: Camelina sativa (L.) Crantz drought resistance, superoxide dismutase, total soluble protein.
Influence of the dose of human umbilical cord mesenchymal stem cells on acute inflammation on the peritonitis model in mice
Authors: Pikus P. A. , Rymar S. E. , Shuvalova N. S. , Kordium V. A. 
Published: November 2019

Aim. Study the effect of the dose of human umbilical cord MSCs on acute inflammation on the peritonitis model in mice with intraperitoneal induction of a 3% solution of proteose peptone. Human umbilical cord MSCs can be used in the cell therapy as allogeneic since their properties provide a minimal risk of an immune response with the transplantation MSCs into body. The inflammation follows all diseases and it is base target for MSCs. Methods. The study is used methods of cell biology. Results. Research has shown that 4 hours after the injection of MSCs, the number of macrophages in the peritoneal cavity of mice immediately decreased. The introduction of 5x103 cells per mouse resulted in 78% decrease the number of macrophages in the exudate. Complete return to a normal has been observed with the injection 70–100 x 103 of cells per mouse. In parallel with the decrease in the number of macrophages, their phagocytic activity has decreased, and these changes also depend on the dose of transplanted MSCs. Conclusions. A dose-dependent decrease of acute inflammation of the abdominal cavity in mice was shown with the introduction of MSCs from the human umbilical cord. The number of macrophages into peritoneal exudate has decreased by 78% in 4 hours after the injection 5x103 cells per mouse. Complete suppression of inflammation has observed with the introduction of 70–100 x 103 cells. Inhibition of inflammation is accompanied by a decrease in the phagocytic activity of macrophages, which indicates a change in their polarization.
Keywords: human umbilical cord MSCs, inflammation, macrophages, phagocytic activity.
Evaluation of the influence of different doses of pre-sowing irradiation of seeds on the content of flavonoids in Hypericum perforatum L. medicinal raw material
Authors: Salivon A. G. , Lystvan K. V. , Litvinov S. V. , Pchelovska S. A. , Shylina Yu. V. , Zhuk V. V. , Tonkal L. V. 
Published: November 2019

Aim. The purpose of this work was to determine the dose of g- and X-ray pre-sowing irradiation of seeds of Hypericum perforatum L., causing an increase in biomass and the accumulation of flavonoids in medicinal raw materials. Methods. X-ray and g-irradiation of seeds, biometric methods, flavonoids extraction, quantification of flavonoid content in extracts using spectrophotometry, qualitative and semi-quantitative analysis of extracts using high-performance liquid chromatography (HPLC), common statistical methods for processing and analysis of data. Results. It was shown that acute X-ray pre-sowing irradiation of H. perforatum seeds at a dose of 20 Gy leads to an increase in the crop of medicinal raw material without losing its pharmaceutical value. Irradiation also increases concentration of quercetin, routine, hyperoside in herbal medicines, grown from seeds, that was chronically irradiated with g-radiation in a total dose of 1 Gy. The HPLC analysis of extracts confirmed that the qualitative composition of ethanol extracts of H. perforatum did not change due to the effect of irradiation on the seeds. Conclusions. The obtained results confirm the possibility of application of pre-sowing irradiation of seeds of H. perforatum in the range of 1–35 Gy in order to increase its productivity and increase the pharmaceutical value of the medicinal raw material.
Keywords: Hypericum perforatum L., ionizing irradiation, productivity, flavonoids.
Some aspects of in vitro wheat biotechnology
Authors: Sergeeva L. E. , Bronnikova L. I. 
Published: November 2019

Aim. Drastic climate changes lead to decrease of the appropriate agricultural plants and stimulate the elaboration of new biotechnologies. The preferences of in vitro system are used for providing the acceleration of the plant selection. The cultivating in vitro is a procedure combined common approaches and special adaptation to plant species. This ideology is essential for all cereals and for wheat in particular. There are several aspects of this ideology: the optimization of cultural conditions; the obtaining wheat cultures and studying distinctive features of their proliferation; the detection parameters of viability, realized on the entire plant level; the comparison of those reactions with cells characteristics. Methods. The standard manipulations of primary explants dissection and several protocols of callus induction and raise are used. Results. Cell cultures of new wheat genotypes were obtained. Those forms were selected in the Institute of Plant Physiology and Genetics NAS of Ukraine. The peculiar features of wheat cell cultures were revealed and investigated. Conclusions. Cell cultures obtained from new genotypes of winter wheat demonstrated common reactions with young plants. Parallel investigations of some biochemical parameters realized on cellular level in cell cultures and plant cells is a possible way to acceleration the genotypes with better characteristics selection.
Keywords: winter wheat, in vitro system, cell culture.
Obtaining of the recombinant rhIL7-BAPmut fusion protein and its functional characterization
Authors: Usenko M. O. , Okunev O. V. , Bentsionova K. I. , Gorbatiuk O. B. , Irodov D. M. , Kordium V. A. 
Published: November 2019

Aim. The purpose was to obtain a functionally active form of the recombinant fusion protein based on the human interleukin-7 (rhIL7) and bacterial alkaline phosphatase (BAPmut). Methods. The DNA sequences encoding rhIL-7 and BAPmut were subcloned into the pET24a(+) plasmid vector containing the 6His-tag sequence for further chromatographic purification of the target protein. The cells of E. coli strain BL21(DE3) were transformed with pET24-rhIL7-BAPmut plasmid vector. Protein synthesis was induced by IPTG and by autoinduction. Bifunctional activity of rhIL7-BAPmut after refolding via dilution is confirmed immunochemically by binding to specific antibodies. Results. RhIL7-BAPmut protein has been designed. It was shown that autoinduction protocol provides a significantly higher level of protein synthesis in E. coli compared with IPTG induction. In vitro method of purification and renaturation of rhIL7-BAPmut from inclusion bodies has been worked out. It has been shown that rhIL7-BAPmut has a specific biological activity after renaturation. Conclusions. The obtained rhIL7-BAPmut protein can be used for screening of immune combinatorial cDNA libraries of immunoglobulins variable genes, that are the sources of specific single chain antibodies. The protein also can be used for qualitative and quantitative analysis of IL-7 receptors.
Keywords: IL-7, BAPmut, inclusion bodies, renaturation.
140 years from the academic V.Ya. Yuriev birthday. Pages of biography in special historical disciplines
Authors: Blume Ya. B. , Barshteyn V. Yu. 
Published: November 2019

Aim. To study and introduce into scientific circulation the material historical sources that are studied by special historical disciplines and relate to the biography of Academician V. Ya. Yuriev. Methods. The paper used both general scientific (historical, logical) and special (subject-chronological, retrospective) methods. Results. One of the reasons for the choice by V. Ya. Yuriev the future specialty, history and authority of the educational institutions in which Yuriev studied have been analyzed. The role of the Kharkiv Society of Agriculture and Agricultural Industry in the development of agricultural science, organization of the Kharkiv breeding station has been highlighted. Briefly analyzed scientific and pedagogical activity of V. Ya. Yuriev. Conclusions. The analysis of material historical sources, objects of faleristics, numismatics (including medallic art), philately of the Russian Empire, USSR, Poland and Ukraine allowed us to find new colors to tell about some of the biography facts of Academician V. Ya. Yuriev
Keywords: V. Ya. Yuriev, history of biology, faleristics, numismatics, philately.
Cloud technologies as a way to form and develop evolutionary concepts in future teachers of biology
Authors: Borodina K. I. , Kmets A. M. , Lutsenko O. I. 
Published: November 2019

Aim. The article is devoted to the substantiation of theoretical and methodical principles of professional training of teachers of biology in the conditions of the informational and educational environment; multilevel and differentiated approaches in training with the possibility of continuous receipt of online and off-line assistance. Methods. Analysis of psychological and pedagogical, methodical literature; synthesis, a generalization; psycho-pedagogical observation, questionnaire, pedagogical experiment. Results. The authors proposed a methodology for involving information technologies, in particular cloud services, the inclusion of their varieties in all activities of the teacher in order to facilitate interaction between the subjects of the educational process, improve the content, forms and ways of interaction of the teacher and students in the process of formation and development of evolutionary concepts. Conclusions. Teaching and methodological support, developed in conditions of cloud-oriented environment, will be open and dynamic, elementarily variable and perceptible for the formation and development of scientific concepts, in particular, evolutionary ones.
Keywords: "cloud technologies", formation, development, evolutionary concepts, means of training.
Charles Darwin, an evolutionary scientist
Authors: Humeniuk H. B. , Chen I. B. , Voloshyn O. S. 
Published: November 2019

Charles Robert Darwin is an English naturalist, geologist and biologist. He was one of the first to realize and clearly demonstrated that all living organisms evolved over time from common ancestors. The main driving force of evolution was called natural selection and uncertain variability. The existence of evolution was recognized by most scientists during the life of Charles Darwin, while his theory of natural selection, as the main explanation of evolution, became universally recognized only in the 30’s of the XX century. The ideas and discoveries of Charles Darwin, in a revised form, form the foundation of a modern synthetic theory of evolution and form the basis of biology, providing a logical explanation for biodiversity. Orthodox followers of Darwin’s teachings develop the direction of the evolutionary thought bearing his name Darwinism.
Keywords: evolution, natural selection, uncertain variability, theory, biodiversity.