bluuebunny/crossref_metadata_2025_split huggingface dataset:
PROFIL HEMATOLOGI TIKUS (<em>Rattus norvegicus</em>) GALUR <em>SPRAGUE-DAWLEY</em> JANTAN UMUR 7 DAN 10 MINGGU
Authors: Rosidah Idah , Ningsih Sri , Renggani Tiya Novita , Efendi Julham , Agustini Kurnia 
Published: November 2020

Hematology Profile of Sprague-Dawley Male Rats (Rattus norvegicus)Â Aged 7 and 10 WeeksCurrently, the normal hematology profile has not been yet available for outbred rats in Indonesia. Most of the researchers use the data of hematology profile from references outside Indonesia in which the breeding environment is different. This study aimed to obtain a hematology profile of Sprague-Dawley male rats aged 7 and 10 weeks. The animals were obtained from the Laboratory of Animal Experiment, NADFC. Blood was drawn from the retro-orbital sinus and then analyzed to obtain the hematology profile including erythrocytes, hemoglobin, hematocrit, MCV, MCH, MCHC, leukocytes. Statistically significant differences for erythrocytes, hemoglobin, MCV, MCHC, thrombocyte, and leukocytes parameters were observed between rats aged 7 and 10 weeks. However, there were no statistically significant differences observed on MCH, thrombocyte, and body weight parameters. Data presented here would be useful as a reference for hematology research as well as in evaluating alterations in blood parameters.Keywords: erythrocyte, hematology, leukocyte, Sprague-Dawley, thrombocyteABSTRAKSaat ini belum banyak tersedia informasi profil hematologi untuk hewan coba tikus yang dikembangbiakkan di Indonesia. Kebanyakan peneliti menggunakan data profil hematologi dari referensi luar Indonesia. Penelitian ini bertujuan untuk mendapatkan data hematologi tikus jantan usia 7 dan 10 minggu.Â Hewan coba yang digunakan adalah tikus jantan galur Sprague-Dawley yang diperoleh dari Laboratorium Hewan Percobaan-BPOM. Sebanyak 60 ekor tikus jantan yang terdiri dari 30 ekor usia 7 minggu dan 30 ekor usia 10 minggu diambil darahnya melalui sinus orbitalis. Selanjutnya, dianalisa jumlah sel eritrosit, hemoglobin, hematokrit, MCV, MCH, MCHC, leukosit. Hasil analisis statistik menunjukkan terdapat perbedaan signifikan antara tikus jantan usia 7 dan 10 minggu untuk parameter jumlah eritrosit, hemoglobin, hematokrit, MCV, MCHC dan leukosit. Tetapi tidak terdapat perbedaan signifikan untuk jumlah MCH, trombosit dan berat badan tikus. Data yang diperoleh dapat digunakan sebagai referensi penelitian yang berhubungan erat dengan profil hematologi dan penyakit kelainan darah lainnya.
BAKTERI ENDOFIT YANG DIISOLASI DARI AKAR <em>Eurycoma longifolia</em> DAN POTENSINYA SEBAGAI PENGENDALI JAMUR PATOGEN TANAMAN
Authors: Sulistiyani Tri Ratna , Meliah Siti , Damayanti . 
Published: November 2020

Eurycoma longifoliaÂ (pasak bumi) is known as a medicinal plant that containsÂ biologically active compounds. Studies on endophytic bacteria associated with pasak bumi andÂ theirÂ biocontrol activitiesÂ have not been widely reported.Â The objective of this study is to isolate potential endophytic bacteria associated with E. longifolia possessing biocontrol activity against plant pathogenic fungi, Sclerotium rolfsii, Fusarium solani, F. oxysporum,Â andÂ Colletotrichum gloeosporioides.Â Endophytic bacteria were isolated from the roots of E. longifolia using the plant piece methodÂ and identifiedÂ based on 16S rRNA genes analysis.Â Antagonist test of bacterial isolates was conductedÂ byÂ dual confrontation method.Â TheÂ mechanisms of fungal growth inhibition were evaluated based on their ability to produce hydrolytic enzymes, antibiotic,Â and volatile organic compounds. Two isolates were obtained and identified as Stenotrophomonas maltophilia (Apb1) and Serratia marcescens (Apb2). Apb2 was able toÂ inhibitÂ the growth ofÂ four tested fungi andÂ showed protease, chitinaseÂ as well asÂ cellulase activities. The crude extract and volatile organic compound produced by Apb2Â wereÂ active against F.Â solaniÂ growth.Keywords: biocontrol, endophytic, Eurycoma longifolia, fungi, inhibition mechanismÂ ABSTRAKEurycoma longifoliaÂ (pasak bumi) dikenal sebagai tanaman obat yang mengandungÂ beberapa senyawa aktif secara biologis. PenelitianÂ mengenaiÂ bakteri endofit yang berasosiasi dengan tanaman pasak bumi berikut aktivitasÂ biokontrolnya belumÂ banyakÂ dilaporkan.Â Penelitian ini bertujuan mengisolasi bakteri endofit potensial dari tanamanÂ E. longifoliaÂ yang memiliki aktivitas biokontrol terhadap empat strain uji jamur patogen tanaman, yaitu Sclerotium rolfsii, Fusarium solani, F. oxysporum, dan Colletotrichum gloeosporioides. Bakteri endofit diisolasi dari akarÂ E. longifolaÂ menggunakan metodeÂ plant piece dan diidentifikasi berdasarkan analisis gen 16S rRNA.Â Uji antagonis isolat bakteriÂ dilakukan dengan metode konfrontasi ganda.Â Mekanisme penghambatan jamur patogen tanaman dievaluasi berdasarkan kemampuannya dalam memproduksi enzim hidrolisis, senyawa antibiotik, dan senyawa organik volatil.Â Dua isolat bakteri endofit berhasil diperoleh dan teridentifikasi sebagaiÂ Stenotrophomonas maltophilia Â (Apb1) dan Serratia marcescens (Apb2). Isolat Apb2 mampu menghambat pertumbuhan keempat jamur yang diujiÂ dan menunjukkan aktivitas protease, kitinase dan selulase. Ekstrak kasar dan senyawa organik volatil yang dihasilkan oleh isolat Apb2 aktif menghambatÂ pertumbuhanÂ F. solani.
Hyperbasites as a factor of geodynamics: the results of the surveys at the Taimyr geophysical site
Authors: Kushnir D.G. 
Published: November 2020

Seismic data indicate the widespread inversion origin of the largest geological structures of the Taimyr Peninsula and record repeated changes of the direction of tectonic movements. The inversion the Yenisei-Khatanga trough led to the formation of a system of megaarchs over the ancient depocenter containing massive hyperbasite intrusions at the Moho level, which are marked by the gravity maximum. These intrusive processes are an important factor that can initiate inversion and contribute significantly to its development under permanent compressional tectonic regime in the region.According to the geophysical data, the development of troughs in the north of Central and Western Siberia is associated with the formation of eclogites under the depocenters, at the Moho level. Consistent with the electrical resistivity anomalies, eclogites are plastic and overlap mantle hyperbasites of lower density that leads to gravitational buoying up of ultramafic formations. This, along with metamorphic and metasomatic processes, acts as an impulse for the uplift in the axial parts of the troughs. Simultaneously, expansion and acceleration of sagging, due to the displacement of plastic eclogites and crystal-mantle mixture from the penetration zone towards the peripheral parts of the sedimentary basin, result in the uncompensated sediment accumulation and the formation of the Neocomian clinoform complex.
De Estándares Mínimos de Calidad a estrategias proyectuales: aportes disciplinares a los parámetros técnicos de la Vivienda Social en Argentina
Authors: Fiscarelli Diego , Bellot Rodolfo 
Published: November 2020

<p>Considerando al proyecto como herramienta fundamental en la producción de conocimiento y a la Investigación Proyectual como un marco epistemológico pertinente para una investigación en arquitectura, este trabajo propone reconocer estrategias proyectuales hacia la adaptabilidad en los Estándares Mínimos de Calidad para Viviendas de Interés Social. Se hipotetiza que el cuerpo normativo no establece variables o indicadores que permitan establecer un patrón de referencia.</p><p>Aunque este trabajo recorta en la mediación de las políticas federales de vivienda en Argentina, en el análisis de la vivienda masiva se encuentra la posibilidad de revisar las pautas proyectuales que aún vigentes, determinan límites para la consideración de la adaptabilidad como factor mínimo de calidad. Como premisa describe además una particular y extendida forma de concebir el proyecto de la Vivienda Social. Parte de las conclusiones exponen que existe una relación directa entre el alcance de los estándares en términos discursivos y su valor técnico-proyectual –estrategias. Reconociendo esta situación como condicionante de la capacidad de los prototipos habitacionales para alcanzar satisfactorios niveles cualitativos, se sugieren entonces adecuaciones a lo establecido desde variables de interés específico para la arquitectura de la vivienda y como parte de los aportes disciplinares.</p>
Construcción con Pallets
Authors: Lufiego Edgardo 
Published: November 2020

<p>Se busca dar una solución a la necesidad acuciante de sectores que no podrían acceder a viviendas por otros medios a la vez que propicia un sistema de reciclado y refuncionalización de elementos residuales de la industria de la construcción.</p><p>Capacitando para la construcción de módulos habitables, a partir de paneles realizados con Pallets de madera recuperados como residuos de obras de construcción.</p><p>Este desarrollo propone la construcción con un costo TENDIENDO A CERO ($ 0)</p><p>En una primera etapa la realización de un techo autoportante.</p><p>Se capacita por cursos presenciales en la zona, San Vicente, A.Korn, Villa Castells en La Plata, y actualmente en el 2020 motivados por el COVID-19 de forma virtual con unos 100 vecinos de las localidades de Magdalena, Coronel Brandsen y Chascomús, distantes las primeras unos 40 km y la ultima unos 75 km de La Plata.</p>
Kotły Beethovenowskie jako partytura braku
Authors: Szczerbowska-Prusevicius Katarzyna 
Published: November 2020

W powieści Kotły Beethovenowskie Michała Choromańskiego kategorią centralną jest brak. Dotyczy on nieobecnych osób, zagubionych przedmiotów, nieuchwytnego znaczenia. Tekst akcentuje go wielokrotnie w autoreferencyjny sposób, np. przez użycie w różnych kontekstach pozornie mało atrakcyjnej metafory „dziura”. Obecność i nieobecność nie stanowią tu binarnej opozycji, ale się ze sobą łączą. Kotły Beethovenowskie są zatem i nie są powieścią kryminalną, Janek jest i nie jest pianistą. Znaki prowadzą w tym tekście swobodną grę z czytelnikiem, odraczając, a nawet niwecząc możliwość dotarcia do przekonującej interpretacji. Kotły Beethovenowskie, które konstytuują w świecie przedstawionym wydarzenia i twierdzenia po to, by je następnie poddać rozbiórce, mogą służyć jako literacka ilustracja dekonstruktywizmu.
Autorka stawia sobie za cel prześledzenie zabiegów Choromańskiego zorientowanych na uwypuklenie kategorii braku i odraczanie sensu oraz wskazanie na ich podobieństwo do niektórych założeń dekonstruktywistycznych. Na szczególną uwagę zasługuje tytułowy motyw kotłów Beethovenowskich, który w powieści uruchamia mechanizm błędu kojarzący się z Derridowską différance.
Alfred Hein: Eine Kompagnie Soldaten/Nasza kompania żołnierzy; Wenn ich meine Hände falte/Kiedy swoje ręce złożę (tłumaczenie). Kilka słów o Alfredzie Heinie i jego wierszach
Authors: Meus Paweł 
Published: November 2020

Źródło: Hein Alfred (1916): Eine Kompagnie Soldaten. In: von Schlözer Leopold (Hg.): Garde-Feld-Post. Jg. 3, Nr. 21, 20 Mai (BnF Gallicia, https://gallica.bnf.fr/ark:/12148/bpt6k9406612x/f8.item [Zugriff am 10.10.2018]): 172.Hein Alfred (1968): Zuhausmusik. Geschichten, Betrachtungen, Briefe und Gedichte. Ein Kranz der Erinnerung an den Dichter und seine oberschlesische Heimat. Hg. Knauer Annke-Margarethe. Augsburg: Oberschlesischer Heimatverlag: 192.
MARKETPLACE LARISUMKM.COM SEBAGAI ALAT PENINGKATAN PROMOSI UMKM DI KOTA SALATIGA
Authors: Setiyawati Nina 
Published: November 2020

ABSTRAKIndustri Kecil Menengah atau Usaha Mikro Kecil dan Menengah (UMKM) di Kota Salatiga sebanyak 1.969 unit usaha dan menyerap 14.647 orang tenaga kerja. Akan tetapi terdapat beberapa hal yang masih perlu ditingkatkan pada UMKM di Kota Salatiga adalah metode promosi, efisiensi serta efektifitas proses bisnis penjualan serta distribusi. Oleh karena itu pada kegiatan Pengabdian Kepada Mayarakat (PKM) dilakukan pembangunan marketplace dengan mitra Dinas Koperasi, Usaha Kecil, dan Menengah (DinkopUKM) Kota Salatiga untuk memberdayakan para pelaku UMKM di Kota Salatiga. Selain itu pada PKM ini juga dilakukan pelatihan kepada para pelaku UMKM se-kota Salatiga terkait penggunaan marketplace yang dibangun. Dari evaluasi kegiatan yang dilakukan dengan metode observasi didapatkan bahwa kemauan para peserta untuk mempelajari dan beradaptasi menggunakan marketplace cukup besar meskipun kebanyakan dari para pelaku UMKM tersebut kurang dalam penguasaan teknologi informasi. Kata kunci: marketplace; UMKM; promosi. ABSTRACTSmall and Medium Industries or Micro, Small and Medium Enterprises (MSMEs) in Salatiga City totaling 1,969 business units and absorbing 14,647 workers. However, there are several things that still need to be improved at MSMEs in Salatiga, namely promotion methods, efficiency and effectiveness of the sales and distribution business processes. Therefore, in the Community Service activity, a marketplace was built with partners from Dinas Koperasi, Usaha Kecil dan Menengah Kota Salatiga (DinkopUKM) to empower MSME subjects in the City of Salatiga. Besides that, this community service also conducted training for MSME subjects throughout the city of Salatiga regarding the use of the marketplace that was built. From the evaluation of activities carried out by the observational method, it was found that the participants' willingness to learn and adapt to using the marketplace was quite large, even though most of the MSME actors were less mastery of information technology. Keywords: marketplace; MSMEs; promotion.
OSMANLI ÇELTİK ÜRETİM SAHASI OLARAK KONRAPA YA DA “OSMANLI SARAYININ NADİDESİ KONURALP PİRİNCİ”
Authors: TAŞ Kenan Ziya 
Published: November 2020

Konrapa XVI. yüzyılda Osmanlı idarî yapısı içinde Anadolu eyâleti veya beylerbeyliğine bağlı bir sancak (livâ) merkezi olan Bolu’nun, on altı nahiyesinden biridir. Henüz tarihî kaynaklar itibarıyla kesin ve açık bir şekilde ispatlanamasa da, nahiyenin adının burada türbesi bulunan ve yörenin fethini gerçekleştiren Konur Alp'tan geldiği açıktır. Konrapa merkezinin, Tahrir Defterlerindeki ifadeyle Nefs-i Konrapa'nın bir diğer adı da Üskübü'dür.
Tahıl grubu içinde yer alan pirinç, mutlak olarak büyüme devresi esnasında çok suya ihtiyaç duyduğundan, ancak coğrafî bölgelerin vadi tabanları içinde yetişme zemini bulmaktadır. Sancak dâhilinde bu şartları taşıyan en müsait bölge olan Konrapa nahiyesinde, pirinç ziraatinin yoğunlaştığı görülmektedir. Konrapa’nın o devirde çeltik üretimi yapılan Anadolu'nun büyük merkezlerinden biri olduğu da anlaşılmaktadır. Sancaktaki çeltik ziraatinin önemi, kanunnâmeye hususi bir madde eklenmesini gerektirmiştir. Konrapa'daki kadar büyük olmasa da Kıbrus ve Yedidivan nahiyelerinde de çeltik ziraati mühim bir yer tuttuğu gibi bu çeltik sahaları padişah hassı olarak ayrılmıştı. Hususi bir işletme halinde görülen ve padişah hassı olan sancaktaki bu çeltik ziraati devletçe de yakından takip edilmekteydi.
DINAMIKA KEBANGSAAN MASYARAKAT PERBATASAN INDONESIA - PAPUA NUGINI DI MUARA TAMI JAYAPURA
Authors: Syuhudi Muh. Irfan 
Published: November 2020

<p class="06AbstrakIndonesia"><span lang="IN">Tulisan ini bertujuan mendeskripsikan kondisi sosial masyarakat perbatasan Indonesia-Papua Nugini di Muara Tami dan mendeskripsikan dinamika paham kebangsaan mereka. Metode pengumpulan data melalui wawancara, observasi, dan studi literatur. Temuan penelitian ini adalah, sebagai berikut: <em>Pertama</em>, infrastruktur di Muara Tami mengalami kemajuan dibanding lima tahun lalu. Akses pelayanan publik telah tersedia, meski masih dalam keterbatasan. Kondisi perekonomian masyarakat perbatasan sejak program transmigrasi dibuka pada 1984, umumnya sejahtera. Infrastruktur juga cukup bagus dibanding lima tahun lalu. Secara ekonomi, masyarakat Indonesia di perbatasan lebih sejahtera dibanding orang Papua Nugini (PNG). Ini pula yang menyebabkan perasaan bangga menjadi bagian dari Negara Indonesia. <em>Kedua</em>, paham kebangsaan masyarakat perbatasan secara umum terlihat cukup bagus, apabila mengacu kepada pengetahuan terhadap Pancasila, UUD 1945, NKRI, dan kebinekaan. Meski begitu, ingatan sosial sebagian Orang Asli Papua (OAP) terhadap Organisasi Papua Merdeka (OPM), masih bertumbuh di kalangan aktivis mahasiswa dan dosen OAP. Adanya kesenjangan ekonomi antara migran pendatang non Papua dengan OAP, sering memicu kecemburuan OAP, yang sewaktu-waktu dapat berdampak kepada ketegangan sosial. Karena itu, nasionalisme bagi OAP, juga adalah menyangkut peningkatan kesejahteraan ekonomi.</span></p>
Pensar en una educación geográfica a partir de la subjetividad, el lenguaje, los imaginarios y la fotografía
Authors: Pérez Luis Guillermo Torres 
Published: November 2020

El espacio geográfico al ser analizado desde diferentes perspectivas, evidencia evidenciar su dinamismo y heterogeneidad. En las últimas décadas, la geografía humana ha entrado en un proceso de renovación epistemológica y metodológica que ha permitido el surgimiento de diversos campos que estudian el espacio geográfico a partir de la subjetividad. Desde esta perspectiva, se desarrolla un proyecto de investigación en el marco del Doctorado Interinstitucional en Educación de la Universidad Pedagógica Nacional adscrito al Grupo de Investigación Geopaideia (Colombia), desde el cual se busca interpretar la ciudad desde el lenguaje fotográfico para comprender la incidencia de los imaginarios urbanos que construyen los estudiantes de educación básica, en el estudio del medio urbano, con el propósito de establecer, desde de la enseñanza de la geografía, estrategias que permitan enseñar y aprender el espacio. El articulo expone entonces las relaciones entre la Educación geográfica y la ciudad, para luego abordar la incidencia de la subjetividad en el estudio del lenguaje y el espacio. Finalmente se reflexiona acerca de las implicaciones de pensar la enseñanza de la ciudad a partir del reconocimiento de los imaginarios urbanos y la composición fotográfica.
Dengue infection during Covid-19 pandemic: A challenge for case management
Authors: Miah Md Asaduzzaman 
Published: November 2020

The coronavirus disease 2019 (Covid-19) has been caused by severe acute respiratory syndrome coronavirus 2 (SARS‑CoV‑2) that declared as an global pandemic by the World Health Organization (WHO).1,2 This ongoing pandemic causes devastation across the world while multiple countries have been facing with another outbreak- Dengue, a known tropical disease.3 Dengue is the most rapidly spreading mosquito-borne viral infection, also considered as a major public health concern. During COVID-19 pandemic, the increasing incidence of dengue become a further threat especially in the dengue-endemic countries of Southeast Asia and Latin America.4 The global burden of dengue is dynamic, estimated 50 million real cases per year across approximately 100 countries.5 Currently, most of the countries are fighting against COVID-19, therefore, further outbreak of dengue has been posed a number of practical challenges to combat the diseases simultaneously.
As dengue cases have been increased during Covid-19 pandemic, both SARS‑CoV‑2 and dengue viruses are co-existing and co-circulating in the environment. Consequently, patients with SARS‑CoV‑2 and dengue co-infection has been reported recently in several countries like Singapore, Thailand, India, and Bangladesh.6-9 Hence, it is speculated that the co-infection cases will be increased and found in another countries in the upcoming days when dengue season goes in its peak. Currently, multiple countries in South America like Brazil, Paraguay, Colombia, Argentina, Bolivia are suffering seriously from co-epidemics of dengue and Covid-19.4
Bangladesh Med Res Counc Bull 2020; 46(2): 145-146
Case Report: Acute Bulbar Palsy Plus Syndrome: A Guillain-Barré Syndrome Variant More Prone to Be a Subtype Than Overlap of Distinct Subtypes
Authors: Cao Qian , Chu Hong , Fu Xiujuan , Yao Jiajia , Xiao Zheman , Lu Zuneng 
Published: November 2020

Objective: Acute bulbar palsy plus (ABPp) syndrome is a rare regional variant of Guillain-Barré syndrome (GBS) characterized by acute bulbar palsy combined with other cranial symptoms or ataxia without limb and neck weakness. We aim to investigate characteristics of ABPp syndrome and analyze its nosological position within the GBS spectrum.Methods: A patient with ABPp syndrome was reported, and previous case reports of patients who met the criteria for ABPp syndrome from the literature were reviewed.Results: A total of 28 patients were included in our study. Median age was 32 years. Most of the patients (78.6%) were from Asia, and 75.0% had preceding infection. The main accompanying symptoms were ophthalmoplegia (85.7%), facial palsy (60.7%), and ataxia (50.0%). There existed asymmetric weakness in the form of unilateral facial palsy (32.1%) and ptosis (3.6%). Approximately half of the patients had albuminocytological dissociation. All the tested patients were seropositive for antiganglioside antibodies, of which the two most common were immunoglobulin G (IgG) anti-GT1a (77.3%) and anti-GQ1b (59.1%) antibodies. Over one-third of the patients who underwent electrophysiological assessment showed subclinical neuropathy beyond cranial nerves. The outcome was generally favorable as 89.3% of patients made full recovery within 5 months.Conclusion: The hitherto largest case series of ABPp syndrome advances our understanding of this disease. Serologically, the presence of IgG anti-GT1a and anti-GQ1b antibodies predicts and contributes to the disease. Phenotypically, ABPp syndrome is more prone to be a separate subtype of GBS than overlap of distinct subtypes and has the potential to complement current diagnostic framework of GBS.
Discovery and Validation of a Metastasis-Related Prognostic and Diagnostic Biomarker for Melanoma Based on Single Cell and Gene Expression Datasets
Authors: Wan Qi , Liu Chengxiu , Liu Chang , Liu Weiqin , Wang Xiaoran , Wang Zhichong 
Published: November 2020

BackgroundSingle cell sequencing can provide comprehensive information about gene expression in individual tumor cells, which can allow exploration of heterogeneity of malignant melanoma cells and identification of new anticancer therapeutic targets.MethodsSingle cell sequencing of 31 melanoma patients in GSE115978 was downloaded from the Gene Expression Omniniub (GEO) database. First, the limma package in R software was used to identify the differentially expressed metastasis related genes (MRGs). Next, we developed a prognostic MRGs biomarker in the cancer genome atlas (TCGA) by combining univariate cox analysis and the least absolute shrinkage and selection operator (LASSO) method and was further validated in another two independent datasets. The efficiency of MRGs biomarker in diagnosis of melanoma was also evaluated in multiple datasets. The pattern of somatic tumor mutation, immune infiltration, and underlying pathways were further explored. Furthermore, nomograms were constructed and decision curve analyses were also performed to evaluate the clinical usefulness of the nomograms.ResultsIn total, 41 MRGs were screened out from 1958 malignant melanoma cell samples in GSE115978. Next, a 5-MRGs prognostic marker was constructed and validated, which show more effective performance for the diagnosis and prognosis of melanoma patients. The nomogram showed good accuracies in predicting 3 and 5 years survival, and the decision curve of nomogram model manifested a higher net benefit than tumor stage and clark level. In addition, melanoma patients can be divided into high and low risk subgroups, which owned differential mutation, immune infiltration, and clinical features. The low risk subgroup suffered from a higher tumor mutation burden (TMB), and higher levels of T cells infiltrating have a significantly longer survival time than the high risk subgroup. Gene Set Enrichment Analysis (GSEA) revealed that the extracellular matrix (ECM) receptor interaction and epithelial mesenchymal transition (EMT) were the most significant upregulated pathways in the high risk group.ConclusionsWe identified a robust MRGs marker based on single cell sequencing and validated in multiple independent cohort studies. Our finding provides a new clinical application for prognostic and diagnostic prediction and finds some potential targets against metastasis of melanoma.