bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Design of Blended Learning Modules for Higher Education
Authors: Cahyono A N , Zaenuri  , Subagja M 
Published: December 2019

Abstract
Blending the traditional values of face-to-face teaching and the best practices of online learning becomes a way to face challenges in the fourth Industrial Revolution era. This study aimed to design blended learning modules for higher education. An explorative design research was conducted engaging lecturers in 69 study programs at Universitas Negeri Semarang. Data was collected through focussed group discussion, observation, a series of pilots and a debriefing session. The results concern standards for elements, regulations and quality assurance. Descriptions of the work processes and results are also explained in this paper. Designing blended learning modules must pay attention to the course material characteristics, learning management system features, supporting facilities, the lecturer roles in preparing and facilitating the courses, students’ activities and their perception. This paper offers an alternative of design of online modules for blended learning in higher education.
The Development of Learning Material of Hybrid Learnings Based to Improve Students Learning Outcomes of Introduction to Microeconomics
Authors: Thamrin  , Aditia R 
Published: December 2019

Abstract
One of the implementations of information and communication technology (ICT) in teaching & learning is through hybrid learning which is a combination of face-to-face instructional methods and online learning to answer the challenges of the fourth industrial revolution. This study aims to determine the effectiveness of learning outcomes Introduction to microeconomics with hybrid learning. This research uses a research and development method. Data collection was carried out by conducting one to one formative evaluation and limited trials and tests after the learning process. The results of this study indicate that hybrid learning teaching materials effectively used in introductory learning in microeconomics. The t-test at α 0.05 stated that the learning outcomes of introduction to microeconomics were taught by hybrid learning is higher than the group of students who were taught by direct learning.
Immunohistochemical Expression of Lymphoid Enhancer Binding Factor 1 in CD5-Positive Marginal Zone, Lymphoplasmacytic, and Follicular Lymphomas
Authors: Patel Nisha , Durkin Lisa , Bodo Juraj , Hsi Eric D 
Published: December 2019

Abstract

Objectives
Lymphoid enhancer binding factor 1 (LEF1) is expressed in most cases of chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL) and has shown utility in distinguishing CLL/SLL from other small B-cell lymphomas. LEF1 expression has not been systematically studied in CD5-positive marginal zone lymphomas (MZLs), lymphoplasmacytic lymphomas (LPLs), and follicular lymphomas (FLs). We evaluated whether these cases lacked LEF1, helping to distinguish them from CLL/SLL.

Methods
MZLs, LPLs, and FLs expressing CD5 were retrospectively studied for expression of LEF1 by immunohistochemistry.

Results
LEF1 was absent in 17 of 18 CD5-positive lymphomas including 13 MZLs (2 nodal, 3 splenic, and 8 mucosa-associated lymphoid tissue lymphomas), 3 LPLs, and 1 of 2 FLs. One grade 3A CD5-positive FL expressed LEF1 in a majority of tumor cells.

Conclusions
LEF1 is not expressed in most CD5-positive MZLs and LPLs; therefore, it is a reliable marker for distinguishing them from CLL/SLL. LEF1 may be expressed in CD5-positive FLs.
A Phase 2, Randomized, Double-blind, Placebo-Controlled Trial of Presatovir for the Treatment of Respiratory Syncytial Virus Upper Respiratory Tract Infection in Hematopoietic-Cell Transplant Recipients
Authors: Chemaly Roy F , Dadwal Sanjeet S , Bergeron Anne , Ljungman Per , Kim Yae-Jean , Cheng Guang-Shing , Pipavath Sudhakar N , Limaye Ajit P , Blanchard Elodie , Winston Drew J , Stiff Patrick J , Zuckerman Tsila , Lachance Silvy , Rahav Galia , Small Catherine B , Mullane Kathleen M , Patron Roberto L , Lee Dong-Gun , Hirsch Hans H , Waghmare Alpana , McKevitt Matt , Jordan Robert , Guo Ying , German Polina , Porter Danielle P , Gossage David L , Watkins Timothy R , Marty Francisco M , Chien Jason W , Boeckh Michael 
Published: December 2019

AbstractBackgroundHematopoietic-cell transplant (HCT) recipients are at risk for severe respiratory syncytial virus (RSV) infection. We evaluated the RSV fusion inhibitor presatovir in a randomized, double-blind, Phase II trial in HCT recipients with RSV upper respiratory tract infections.MethodsPatients were stratified by lymphopenia (&lt;200/µL) and ribavirin use; were randomized, stratified by lymphopenia (&lt;200/μL) and ribavirin use, to receive oral presatovir at 200 mg or a placebo on Days 1, 5, 9, 13, and 17, and were followed through Day 28. The coprimary efficacy endpoints were the time-weighted average change in the nasal RSV viral load between Days 1 and 9 and the proportion of patients developing lower respiratory tract complications (LRTCs) through Day 28.ResultsFrom 23 January 2015 to 16 June 2017, 189 patients were randomly assigned to treatment (96 to presatovir and 93 to the placebo). Presatovir treatment, compared with the placebo treatment, did not significantly affect (prespecified α = 0.01) a time-weighted average decline in the RSV viral load from Day 1 to 9 (treatment difference, −0.33 log10 copies/mL; 95% confidence interval [CI] −.64 to −.02 log10 copies/mL; P = .040) or the progression to LRTC (11.2% vs 19.5%, respectively; odds ratio, 0.50; 95% CI, .22–1.18; P = .11). In a post hoc analysis among patients with lymphopenia, presatovir decreased LRTC development by Day 28 (2/15 [13.3%] vs 9/14 [64.3%], respectively; P = .008), compared with the placebo. Adverse events were similar for patients receiving presatovir and the placebo.ConclusionsPresatovir had a favorable safety profile in adult HCT recipients with RSV but did not achieve the coprimary endpoints. Exploratory analyses suggest an antiviral effect among patients with lymphopenia.Clinical Trials RegistrationNCT02254408; EUDRA-CT#2014-002474-36.
Sudden cardiac death among persons with diabetes aged 1–49 years: a 10-year nationwide study of 14 294 deaths in Denmark
Authors: Lynge Thomas Hadberg , Svane Jesper , Pedersen-Bjergaard Ulrik , Gislason Gunnar , Torp-Pedersen Christian , Banner Jytte , Risgaard Bjarke , Winkel Bo Gregers , Tfelt-Hansen Jacob 
Published: December 2019

Abstract

Aims
The aim of this study was to compare nationwide incidence rate (IR) of sudden cardiac death (SCD) in persons aged 1–49 years with and without diabetes mellitus (DM).

Methods and results
The study population consisted of all persons in Denmark aged 1–49 years in 2000–09, which equals 27.1 million person-years. All 14 294 deaths in the 10-year period were included. By using the highly descriptive Danish death certificates, 1698 cases of sudden and unexpected death were identified. Through review of autopsy reports, discharge summaries, and the Danish registries, we identified 1363 cases of SCD. The Danish Register of Medicinal Product Statistics was used to identify persons with type 1 DM and type 2 DM. Among the 14 294 decedents, there were 669 with DM, of which 118 suffered SCD (9% of all SCD), making SCD the leading cause of death among young persons with DM. Among those aged 1–35 years, the IR of SCD-DM was 21.9 per 100 000 person-years compared to 2.6 per 100 000 person-years among persons without DM [IR ratio 8.6, 95% confidence interval (CI) 5.8–28.6]. Within the age range 36–49 years, the IR among persons with DM was 119.8 per 100 000 person-years compared to 19.7 per 100 000 person-years among persons without DM (IR ratio 6.1, 95% CI 4.7–7.8).

Conclusion
We found that young persons with DM aged 1–35 years had &gt;8-fold higher SCD IR compared to young persons without DM. Our study highlights the need for early cardiovascular risk monitoring and assessment in young persons with DM.
Microarray analyses of closely related glycoforms reveal different accessibilities of glycan determinants on N-glycan branches
Authors: Li Lei , Guan Wanyi , Zhang Gaolan , Wu Zhigang , Yu Hai , Chen Xi , Wang Peng G 
Published: December 2019

AbstractGlycans mediate a wide variety of biological roles via recognition by glycan-binding proteins (GBPs). Comprehensive knowledge of such interaction is thus fundamental to glycobiology. While the primary binding feature of GBPs can be easily uncovered by using a simple glycan microarray harboring limited numbers of glycan motifs, their fine specificities are harder to interpret. In this study, we prepared 98 closely related N-glycoforms that contain 5 common glycan epitopes which allowed the determination of the fine binding specificities of several plant lectins and anti-glycan antibodies. These N-glycoforms differ from each other at the monosaccharide level and were presented in an identical format to ensure comparability. With the analysis platform we used, it was found that most tested GBPs have preferences toward only one branch of the complex N-glycans, and their binding toward the epitope-presenting branch can be significantly affected by structures on the other branch. Fine specificities described here are valuable for a comprehensive understanding and applications of GBPs.
Fenofibrate rapidly decreases hepatic lipid and glycogen storage in neonatal mice with glycogen storage disease type Ia
Authors: Yavarow Zollie A , Kang Hye-Ri , Waskowicz Lauren R , Bay Boon-Huat , Young Sarah P , Yen Paul M , Koeberl Dwight D 
Published: December 2019

AbstractGlycogen storage disease type Ia (GSD Ia) is caused by autosomal mutations in glucose-6-phosphatase α catalytic subunit (G6PC) and can present with severe hypoglycemia, lactic acidosis and hypertriglyceridemia. In both children and adults with GSD Ia, there is over-accumulation of hepatic glycogen and triglycerides that can lead to steatohepatitis and a risk for hepatocellular adenoma or carcinoma. Here, we examined the effects of the commonly used peroxisomal proliferated activated receptor α agonist, fenofibrate, on liver and kidney autophagy and lipid metabolism in 5-day-old G6pc −/− mice serving as a model of neonatal GSD Ia. Five-day administration of fenofibrate decreased the elevated hepatic and renal triglyceride and hepatic glycogen levels found in control G6pc −/− mice. Fenofibrate also induced autophagy and promoted β-oxidation of fatty acids and stimulated gene expression of acyl-CoA dehydrogenases in the liver. These findings show that fenofibrate can rapidly decrease hepatic glycogen and triglyceride levels and renal triglyceride levels in neonatal G6pc −/− mice. Moreover, since fenofibrate is an FDA-approved drug that has an excellent safety profile, our findings suggest that fenofibrate could be a potential pharmacological therapy for GSD Ia in neonatal and pediatric patients as well as for adults. These findings may also apply to non-alcoholic fatty liver disease, which shares similar pathological and metabolic changes with GSD Ia.
Occupational exposures and odds of gastric cancer: a StoP project consortium pooled analysis
Authors: Shah Shailja C , Boffetta Paolo , Johnson Kenneth C , Hu Jinfu , Palli Domenico , Ferraroni Monica , Tsugane Shoichiro , Hamada Gerson Shigueaki , Hidaka Akihisa , Zaridze David , Maximovich Dmitry , Vioque Jesus , Navarrete-Munoz Eva M , Zhang Zuo-Feng , Mu Lina , Boccia Stefania , Pastorino Roberta , Kurtz Robert C , Rota Matteo , Bonzi Rossella , Negri Eva , La Vecchia Carlo , Pelucchi Claudio , Hashim Dana 
Published: December 2019

Abstract

Background
Gastric cancer pathogenesis represents a complex interaction of host genetic determinants, microbial virulence factors and environmental exposures. Our primary aim was to determine the association between occupations/occupational exposures and odds of gastric cancer.

Methods
We conducted a pooled-analysis of individual-level data harmonized from 11 studies in the Stomach cancer Pooling Project. Multivariable logistic regression was used to estimate the odds ratio (OR) of gastric cancer adjusted for relevant confounders.

Results
A total of 5279 gastric cancer cases and 12 297 controls were analysed. There were higher odds of gastric cancer among labour-related occupations, including: agricultural and animal husbandry workers [odds ratio (OR) 1.33, 95% confidence interval (CI): 1.06–1.68]; miners, quarrymen, well-drillers and related workers (OR 1.70, 95% CI: 1.01–2.88); blacksmiths, toolmakers and machine-tool operators (OR 1.41, 95% CI: 1.05–1.89); bricklayers, carpenters and construction workers (OR 1.30, 95% CI: 1.06–1.60); and stationary engine and related equipment operators (OR 6.53, 95% CI: 1.41–30.19). The ORs for wood-dust exposure were 1.51 (95% CI: 1.01–2.26) for intestinal-type and 2.52 (95% CI: 1.46–4.33) for diffuse-type gastric cancer. Corresponding values for aromatic amine exposure were 1.83 (95% CI: 1.09–3.06) and 2.92 (95% CI: 1.36–6.26). Exposure to coal derivatives, pesticides/herbicides, chromium, radiation and magnetic fields were associated with higher odds of diffuse-type, but not intestinal-type gastric cancer.

Conclusions
Based on a large pooled analysis, we identified several occupations and related exposures that are associated with elevated odds of gastric cancer. These findings have potential implications for risk attenuation and could be used to direct investigations evaluating the impact of targeted gastric cancer prevention/early detection programmes based on occupation.
Emergence of ceftriaxone-resistant Neisseria gonorrhoeae strains harbouring a novel mosaic penA gene in China
Authors: Xiu Leshan , Yuan Qianqin , Li Yamei , Zhang Chi , Tang Lingli , Peng Junping 
Published: December 2019

Abstract

Objectives
The continuous emergence of ceftriaxone-resistant Neisseria gonorrhoeae strains threatens the effectiveness of current treatment regimens for gonorrhoea. The objective of the present study was to characterize three ceftriaxone-resistant N. gonorrhoeae strains with a novel mosaic penA allele isolated in China.

Methods
Three ceftriaxone-resistant Neisseria gonorrhoeae strains (GC150, GC161 and GC208) isolated in 2017 were characterized by N. gonorrhoeae multiantigen sequence typing (NG-MAST), MLST and N. gonorrhoeae sequence typing for antimicrobial resistance (NG-STAR). Recombination analyses were performed using the SimPlot software.

Results
Three strains had the same antibiotic resistance profiles, with resistance to ceftriaxone (MIC 0.5 mg/L), ciprofloxacin (MIC 8.0 mg/L), penicillin (MIC 2.0 mg/L) and tetracycline (MIC 2.0–8.0 mg/L). STs were assigned as MLST7360, NG-MAST14292 and NG-STAR1611/NG-STAR1612. The penA gene of these three strains differed from previous ceftriaxone-resistant gonococcal strains and harboured a novel mosaic allele (penA-121.001). Like N. gonorrhoeae FC428, a widely disseminated ceftriaxone-resistant strain that was initially described in Japan in 2015, all strains also possessed substitutions A311V and T483S in PBP2, which are associated with resistance to ceftriaxone. Potential recombination events were detected in penA between N. gonorrhoeae strain FC428 and commensal Neisseria species. Our results provide further evidence that the commensal Neisseria species (Neisseria cinerea and Neisseria perflava) can serve as a reservoir of ceftriaxone resistance-mediating penA sequences in clinical gonococcal strains.

Conclusions
The emergence of such strains may be the result of the interspecies recombination of penA genes between N. gonorrhoeae strain FC428 and commensal Neisseria species.
Japanese structure survey of radiation oncology in 2012
Authors: Numasaki Hodaka , Teshima Teruki , Ando Yutaka , Akuta Keizo , Ikeda Hiroshi , Okajima Kaoru , Kumano Tomoyasu , Sasaki Tomonari , Sekiguchi Kenji , Tago Masao , Terahara Atsuro , Nakamura Katsumasa , Nishimura Tetsuo , Ogawa Kazuhiko ,   for Society Japanese Radiation Oncology Database Committee
Published: December 2019

ABSTRACT
This paper describes the ongoing structure of radiation oncology in Japan in terms of equipment, personnel, patient load and geographic distribution to identify and overcome any existing limitations. From March 2013 to August 2016, the Japanese Society for Radiation Oncology conducted a questionnaire based on the Japanese national structure survey of radiation oncology in 2012. Data were analyzed based on the institutional stratification by the annual number of new patients treated with radiotherapy per institution. The estimated annual numbers of new and total (new plus repeat) patients treated with radiation were 213 000 and 251 000, respectively. Additionally, the estimated cancer incidence was 865 238 cases with ~24.6% of all newly diagnosed patients being treated with radiation. The types and numbers of treatment devices actually used included linear accelerator (LINAC; n = 864), telecobalt (n = 0), Gamma Knife (n = 44), 60Co remote afterloading system (RALS; n = 23) and 192Ir RALS (n = 130). The LINAC system used dual-energy functions in 651 units, 3D conformal radiotherapy functions in 759 and intensity-modulated radiotherapy (IMRT) functions in 466. There were 792 Japan Radiological Society/Japanese Society for Radiation Oncology-certified radiation oncologists, 1061.6 full-time equivalent (FTE) radiation oncologists, 2124.2 FTE radiotherapy technologists, 181.3 FTE medical physicists, 170.9 FTE radiotherapy quality managers and 841.5 FTE nurses. The frequency of IMRT use significantly increased during this time. In conclusion, the Japanese structure of radiation oncology has clearly improved in terms of equipment and utility although there was a shortage of personnel in 2012.
Role of chromatin modification and remodeling in stem cell regulation and meristem maintenance in Arabidopsis
Authors: Singh Sharmila , Singh Alka , Singh Archita , Mahima  , Yadav Sandeep , Bajaj Ishita , Kumar Shailendra , Jain Ajay , Sarkar Ananda K 
Published: December 2019

AbstractIn higher plants, pluripotent stem cells reside in the specialized microenvironment called stem cell niches (SCNs) harbored at the shoot apical meristem (SAM) and root apical meristem (RAM), which give rise to the aerial and underground parts of a plant, respectively. The model plant Arabidopsis thaliana (Arabidopsis) has been extensively studied to decipher the intricate regulatory mechanisms involving some key transcriptions factors and phytohormones that play pivotal roles in stem cell homeostasis, meristem maintenance, and organ formation. However, there is increasing evidence to show the epigenetic regulation of the chromatin architecture, gene expression exerting an influence on an innate balance between the self-renewal of stem cells, and differentiation of the progeny cells to a specific tissue type or organ. Post-translational histone modifications, ATP-dependent chromatin remodeling, and chromatin assembly/disassembly are some of the key features involved in the modulation of chromatin architecture. Here, we discuss the major epigenetic regulators and illustrate their roles in the regulation of stem cell activity, meristem maintenance, and related organ patterning in Arabidopsis.
The isothermal evolution of a shock-filament interaction
Authors: Goldsmith K J A , Pittard J M 
Published: December 2019

ABSTRACTStudies of filamentary structures that are prevalent throughout the interstellar medium are of great significance to a number of astrophysical fields. Here, we present 3D hydrodynamic simulations of shock-filament interactions where the equation of state has been softened to become almost isothermal. We investigate the effect of such an isothermal regime on the interaction (where both the shock and filament are isothermal), and we examine how the nature of the interaction changes when the orientation of the filament, the shock Mach number, and the filament density contrast are varied. We find that only sideways-oriented filaments with a density contrast of 102 form a three-rolled structure, dissimilar to the results of a previous study. Moreover, the angle of orientation of the filament plays a large role in the evolution of the filament morphology: the greater the angle of orientation, the longer and less turbulent the wake. Turbulent stripping of filament material leading to fragmentation of the core occurs in most filaments; however, filaments orientated at an angle of 85° to the shock front do not fragment and are longer lived. In addition, values of the drag time are influenced by the filament length, with longer filaments being accelerated faster than shorter ones. Furthermore, filaments in an isothermal regime exhibit faster acceleration than those struck by an adiabatic shock. Finally, we find that the drag and mixing times of the filament increase as the angle of orientation of the filament is increased.
Can tides disrupt cold dark matter subhaloes?
Authors: Errani Raphaël , Peñarrubia Jorge 
Published: December 2019

ABSTRACT
The clumpiness of dark matter on sub-kpc scales is highly sensitive to the tidal evolution and survival of subhaloes. In agreement with previous studies, we show that N-body realisations of cold dark matter subhaloes with centrally-divergent density cusps form artificial constant-density cores on the scale of the resolution limit of the simulation. These density cores drive the artificial tidal disruption of subhaloes. We run controlled simulations of the tidal evolution of a single subhalo where we repeatedly reconstruct the density cusp, preventing artificial disruption. This allows us to follow the evolution of the subhalo for arbitrarily large fractions of tidally stripped mass. Based on this numerical evidence in combination with simple dynamical arguments, we argue that cuspy dark matter subhaloes cannot be completely disrupted by smooth tidal fields. Modelling stars as collisionless tracers of the underlying potential, we furthermore study the tidal evolution of Milky Way dwarf spheroidal galaxies. Using a model of the Tucana III dwarf as an example, we show that tides can strip dwarf galaxies down to sub-solar luminosities. The remnant micro-galaxies would appear as co-moving groups of metal-poor, low-mass stars of similar age, embedded in sub-kpc dark matter subhaloes.