bluuebunny/crossref_metadata_2025_split huggingface dataset:
DETECÇÃO DE DNA DE NEOSPORA CANINUM EM PARDELA-PRETA (PROCELLARIA AEQUINOCTIALIS) - RELATO DE CASO
Authors: Sato Ana Paula , Emmerich da Silva Tiffany Christiny , Wilhelm Dilgerc Sanches Adrien , Konell Aline Luiza , Moreira da Fonseca Flávia , Locatelli Dittrich Rosangela 
Published: December 2020

A pardela-preta (Procellaria aequinoctialis) é uma ave procellariiforme, possui distribuição circumpolar ao longo de todo o oceano austral e migra para o hemisfério sul durante o inverno. É considerada uma espécie globalmente vulnerável pela União Mundial para a Conservação da Natureza (IUCN, 2020), devido a mortalidade acidental da espécie na pesca com espinhel e a degradação do seu habitat natural causando rápido declínio populacional. Neospora caninum é um protozoário intracelular formador de cisto que infecta aves e mamíferos. Os canídeos domésticos e silvestres são os hospedeiros definitivos e eliminam os oocistos nas fezes. As aves se infectam pela ingestão de alimentos e água contaminados com oocistos esporulados e por meio da ingestão de cistos em tecidos infectados (DONAHOE et al.,2015). O objetivo do presente estudo foi relatar a presença de DNA de Neospora caninum no coração de uma ave pardela-preta (P. aequinoctialis), fêmea e adulta. Amostras de tecidos (musculatura peitoral, cérebro e coração) foram coletadas para a realização do diagnóstico molecular de N. caninum e acondicionadas e fixadas em formalina 10% tamponada para análise histopatológica. A extração de DNA foi realizada com kit comercial. Para a amplificação de DNA N. caninum foi empregada a reação de cadeia da polimerase nested-PCR com os primers JS4, CT2c, JS4b e CT2b, que amplificam a região ITS1. Foi detectado DNA de N. caninum na amostra do coração. O produto da PCR foi submetido a purificação e sequenciamento. Foi obtida sequência nucleotídica (MW023246) que apresentou identidade de 99% com isolados de N. caninum depositados no Genbank. Na análise histopatológica os tecidos não apresentavam alteração macroscópica e a análise microscópica revelou congestão no miocárdio. O presente relato demonstra que a pardela-preta (P. aequinoctialis) pode ser naturalmente infectada por N. caninum e até o momento é primeira descrição nesta espécie de ave. Existem poucos estudos sobre a ocorrência de N. caninum na fauna marinha e as possíveis fontes de infecção ainda não foram bem elucidadas. Estudos demonstraram que mamíferos marinhos (lontras, morsas e golfinhos) estão expostos ao protozoário indicando uma possível contaminação das águas marinhas por oocistos O achado nesse relato sugere que as aves marinhas podem ser potenciais hospedeiros intermediários de N. caninum, atuando como sentinela e indicador de contaminação do ambiente marinho, além da sua participação no ciclo silvestre do parasito.
De novo small deletion affecting transcription start site of short isoform of AUTS2 gene in a patient with syndromic neurodevelopmental defects
Authors: Martinez‐Delgado Beatriz , Lopez‐Martin Estrella , Lara‐Herguedas Julián , Monzon Sara , Cuesta Isabel , Juliá Miguel , Aquino Virginia , Rodriguez‐Martin Carlos , Damian Alejandra , Gonzalo Irene , Gomez‐Mariano Gema , Baladron Beatriz , Cazorla Rosario , Iglesias Gema , Roman Enriqueta , Ros Purificacion , Tutor Pablo , Mellor Susana , Jimenez Carlos , Cabrejas Maria Jose , Gonzalez‐Vioque Emiliano , Alonso Javier , Bermejo‐Sánchez Eva , Posada Manuel 
Published: December 2020

AbstractDisruption of the autism susceptibility candidate 2 (AUTS2) gene through genomic rearrangements, copy number variations (CNVs), and intragenic deletions and mutations, has been recurrently involved in syndromic forms of developmental delay and intellectual disability, known as AUTS2 syndrome. The AUTS2 gene plays an important role in regulation of neuronal migration, and when altered, associates with a variable phenotype from severely to mildly affected patients. The more severe phenotypes significantly correlate with the presence of defects affecting the C‐terminus part of the gene. This article reports a new patient with a syndromic neurodevelopmental disorder, who presents a deletion of 30 nucleotides in the exon 9 of the AUTS2 gene. Importantly, this deletion includes the transcription start site for the AUTS2 short transcript isoform, which has an important role in brain development. Gene expression analysis of AUTS2 full‐length and short isoforms revealed that the deletion found in this patient causes a remarkable reduction in the expression level, not only of the short isoform, but also of the full AUTS2 transcripts. This report adds more evidence for the role of mutated AUTS2 short transcripts in the development of a severe phenotype in the AUTS2 syndrome.
Capturing nested information from disordered peptide phases
Authors: Gordon Christella K. , Luu Regina , Lynn David 
Published: December 2020

AbstractAnfinsen's dogma emerged from experiments which show that a protein's native 3D folded architecture is encoded within its primary sequence. This insight emerged before almost 40% of eukaryotic proteins were found to contain intrinsically disordered domains with binding partners that are able to template multiple structures and functional outputs. Despite the challenges associated with characterizing such intrinsically disordered protein domains and their interactions with various binding partners, the need to understand how they propagate and regulate context‐dependent information now becomes increasingly important. In addition to understanding the complex signaling networks of multicellular organisms, harness their potential will expand the next generation of functional materials. Accordingly, we review progress with model peptides to reveal the rules necessary for precise spatiotemporal assembly of polypeptide materials.
Optimal filter design for cyber‐physical systems under stealthy hybrid attacks
Authors: Li Yuman , Lin Hong , Lam James 
Published: December 2020

AbstractThis article studies the optimal filtering problem for cyber‐physical systems under stealthy hybrid cyber‐attacks. Due to the stealthiness of cyber‐attacks, the optimal filter (OF) consists of an exponentially increasing number of terms, which makes it computationally infeasible in practice. To address this computational issue, an optimal linear filter (OLF) is developed with its estimation performance fairly close to the optimal one. Furthermore, for a system under the stealthy hybrid cyber‐attacks, it is proved that when the system is stable, the exponentially increasing number of terms remain bounded, making the OF stable; and that the OLF is stable if and only if the system is stable. Finally, numerical examples are employed to illustrate the effectiveness of the proposed two filters.
MICRO-CT Imaging of Thiel-Embalmed and Iodine-Stained Human Temporal Bone for 3D Modeling
Authors: Sebastian Halm , David Haberthuer , Elisabeth Eppler , Valentin Djonov , Andreas Arnold 
Published: December 2020

ABSTRACT

OBJECTIVE
The following study investigates whether human Thiel-embalmed temporal bones are suitable for generating an accurate and complete data set with micro-computed tomography (micro-CT) and the effect of solid iodine staining for improved visualization and facilitated segmentation of middle ear structures.

METHODS
One temporal bone was used to verify the accuracy of the imaging by measuring the stapes digitally on the tomography images and physically under the microscope after removal from the temporal bone. All measurements were compared with literature values.
The contralateral temporal bone was used to evaluate segmentation and 3D modeling after iodine staining and micro-CT scanning.

RESULTS
The digital and physical stapes measurements differ up to 0.17 mm and 24% but correlate well with the literature values. Soft tissue structures were visible in the unstained scan. However, iodine staining increased the contrast-to-noise ratio by a factor 3.7 on average. The 3D model depicts all ossicles and soft tissue structures in detail, including the chorda tympani, which was not visible in the unstained scan.

CONCLUSION
Micro-CT imaging of Thiel-embalmed temporal bones accurately represents the entire anatomy. Iodine staining considerably increases the contrast of soft tissues, simplifies segmentation and enables detailed 3D modeling of the middle ear, which is suitable for further use as a finite element model (FEM).
Donor HLA class 1 evolutionary divergence is a major predictor of liver allograft rejection: a retrospective cohort study
Authors: Féray Cyrille , Taupin Jean-Luc , Sebagh Mylène , Allain Vincent , Demir Zeynep , Allard Marc-Antoine , Desterke Christophe , Coilly Audrey , Saliba Faouzi , Vibert Eric , Azoulay Daniel , Guettier Catherine , Debray-Devictor Dominique , Caillat-Zucman Sophie , Samuel Didier 
Published: December 2020

AbstractBackgroundRecognition of donor antigens by the recipient’s immune system leads to allograft rejection. HLA evolutionary divergence (HED) between an individual’s HLA alleles is a continuous metric that quantifies the differences between each amino acid of two homologous alleles and reflects the breadth of the immunopeptidome presented to T lymphocytes. We investigated whether or not HED of the donor or of the recipient has an impact on liver transplant rejection.MethodWe did a retrospective cohort study in 1154 adult and 113 children recipients of liver transplant. We considered the histological lesions in liver biopsies performed routinely 1,2 and 5 years after transplantation and in case of liver dysfunction. Donor-specific anti-HLA antibodies (DSA) were determined in children at the time of biopsy. HED was calculated using the physicochemical Grantham distance for class I (HLA-A, HLA-B) and class II (HLA-DRB1, HLA-DQB1) alleles. We assessed the incidence of rejection-related liver lesions using a multivariate Cox proportional hazards regression analysis.FindingsIn the adult cohort, recipients from donors with class I HED above the median had a higher risk of acute or chronic rejection, but not of other histological lesions. HED of the recipients was not related to any histological lesion. In multivariate analysis, a high donor class I HED was associated with acute rejection (hazard ratio [HR] 1.79; 95% confidence interval [CI]: 1.34-2.40; P<0.0001) or chronic rejection (HR 2.26, CI 1.45-3.51; P<0.0001) and was independent of age and HLA identities. In the pediatric cohort, class I HED of the donor was also associated with acute rejection (HR 1.81, 95% CI 1.12-3.14; P=0.013) independently of the presence of DSA.InterpretationClass I HED of the donor reflects graft immunogenicity and predicts rejection independently of donor-recipient HLA compatibility. This novel and easily accessible prognostic marker could improve donor selection and guide immunosuppression.
Negative emotions and consumers’ preventive health behavior: a multi-method exploratory study
Authors: Ben Ayed Manel , El Aoud Nibrass 
Published: December 2020

Purpose
As the emotional/experiential aspect is still marginalized in the literature, this paper aims to explore the role of negative emotions associated with past experiences (PEs) in explaining the preventive health behavior (PHB) of the consumers with a chronic disease.

Design/methodology/approach
From the perspective of experiential marketing, a multi-method exploratory study was conducted among consumers with type 2 diabetes. The investigation process applied phenomenological interviews, visual projections and consumer diaries to adequately capture the emotional responses related to consumer experience of healthy diet behavior.

Findings
The results of a methodological triangulation show that the generation of negative emotions associated with PE with the PHB stimulates adopting healthy eating behavior rather than restraining it.

Research limitations/implications
The multi-method protocol chosen for this experiential study is a response to the methodological requirements for a broader conception of consumer experience in the context of chronic diseases. This protocol can be used for further empirical investigation of emotional reactions experienced in consumer behavior in the broad sense.

Practical implications
Managerial implications are provided for health-care professionals on how to implement marketing strategies and practices based on the identified consumer profiles.

Originality/value
This study highlights the significant role of experienced emotional responses in explaining the adoption of the PHB and underlines that not all decisions are rational. It also contributes to the literature by specifying a mixed-method approach of data collection for deeper investigation of the consumer’s emotional responses to the health behavior experience.
The gammaproteobacterium Achromatium forms intracellular amorphous calcium carbonate and not (crystalline) calcite
Authors: Benzerara Karim , Bolzoni Romain , Monteil Caroline , Beyssac Olivier , Forni Olivier , Alonso Béatrice , Asta Maria P. , Lefevre Christopher 
Published: December 2020

AbstractAchromatium is a long known uncultured giant gammaproteobacterium forming intracellular CaCO3 that impacts C and S geochemical cycles functioning in some anoxic sediments and at oxic‐anoxic boundaries. While intracellular CaCO3 granules have first been described as Ca oxalate then colloidal CaCO3 more than one century ago, they have often been referred to as crystalline solids and more specifically calcite over the last 25 years. Such a crystallographic distinction is important since the respective chemical reactivities of amorphous calcium carbonate (ACC) and calcite, hence their potential physiological role and conditions of formation, are significantly different. Here, we analyzed the intracellular CaCO3 granules of Achromatium cells from Lake Pavin using a combination of Raman microspectroscopy and scanning electron microscopy. Granules in intact Achromatium cells were unequivocally composed of ACC. Moreover, ACC spontaneously transformed into calcite when irradiated at high laser irradiance during Raman analyses. Few ACC granules also transformed spontaneously into calcite in lysed cells upon cell death and/or sample preparation. Overall, the present study supports the original claims that intracellular Ca‐carbonates in Achromatium are amorphous and not crystalline. In that sense, Achromatium is similar to a diverse group of Cyanobacteria and a recently discovered magnetotactic alphaproteobacterium, which all form intracellular ACC. The implications for the physiology and ecology of Achromatium are discussed. Whether the mechanisms responsible for the preservation of such unstable compounds in these bacteria are similar to those involved in numerous ACC‐forming eukaryotes remains to be discovered. Last, we recommend to future studies addressing the crystallinity of CaCO3 granules in Achromatium cells recovered from diverse environments all over the world to take care of the potential pitfalls evidenced by the present study.
Swimming training improves cardiovascular autonomic dysfunctions and prevents renal damage in rats fed a high‐sodium diet from weaning
Authors: de Souza Jaqueline Aparecida , Becker Lenice Kappes , Batista Marcos Adriano Carlos , de Assis Braga Daiane Cristina , Gomes Paula Magalhães , Alzamora Andréia Carvalho , Vieira Maria Aparecida Ribeiro , de Lima Wanderson Geraldo , Andrade Marina Gonçalves Caetano , de Lima Sanches Bruno , Totou Nádia Lúcia , de Assis Dias Martins Júnior Francisco , de Oliveira Lisandra Brandino , Antunes Vagner Roberto , Cardoso Leonardo Máximo 
Published: December 2020

New Findings

What is the central question of this study?
How does swimming exercise training impact hydro‐electrolytic balance, renal function, sympathetic contribution to resting blood pressure and cerebrospinal fluid (CSF) [Na+] in rats fed a high‐sodium diet from weaning?

What is the main finding and its importance?
An exercise‐dependent reduction in blood pressure was associated with decreased CSF [Na+], sympathetically driven vasomotor tonus and renal fibrosis indicating that the anti‐hypertensive effects of swimming training in rats fed a high‐sodium diet might involve neurogenic mechanisms regulated by sodium levels in the CSF rather than changes in blood volume.

AbstractHigh sodium intake is an important factor associated with hypertension. High‐sodium intake with exercise training can modify homeostatic hydro‐electrolytic balance, but the effects of this association are mostly unknown. In this study, we sought to investigate the effects of swimming training (ST) on cerebrospinal fluid (CSF) Na+ concentration, sympathetic drive, blood pressure (BP) and renal function of rats fed a 0.9% Na+ (equivalent to 2% NaCl) diet with free access to water for 22 weeks after weaning. Male Wistar rats were assigned to two cohorts: (1) fed standard diet (SD) and (2) fed high‐sodium (HS) diet. Each cohort was further divided into trained and sedentary groups. ST normalised BP levels of HS rats as well as the higher sympathetically related pressor activity assessed by pharmacological blockade of ganglionic transmission (hexamethonium). ST preserved the renal function and attenuated the glomerular shrinkage elicited by HS. No change in blood volume was found among the groups. CSF [Na+] levels were higher in sedentary HS rats but were reduced by ST. Our findings showed that ST effectively normalised BP of HS rats, independent of its effects on hydro‐electrolytic balance, which might involve neurogenic mechanisms regulated by Na+ levels in the CSF as well as renal protection.
Structural Heterogeneity in Europe
Authors: Hürtgen Stefanie 
Published: December 2020

Abstract
Structural heterogeneity is probably the most important category of dependency theory to characterize the “development of underdevelopment” in the Global South. Should it therefore be a provocation to speak of structural heterogeneity in Europe? No, this article argues, because with reference to Osvaldo Sunkel, Fred Scholz and Neil Brenner among others, the political-economic logic of transnational economic integration based on socio-spatial fragmentation now also encompasses the Global North; or more specifically: the structural logic of economic transnationalisation and sociospatial fragmentation constitutes the core dynamics of European integration. So, the critical development debates of the 1970s should be rediscovered as pioneering concepts and updated in terms of spatial theory in the sense of a local solidarity perspective.
Regularized error function-based extended Kalman filter for estimating the cancer chemotherapy dosage: impact of improved grey wolf optimization
Authors: Mohite Utkarsha L. , Patel Hirenkumar G. 
Published: December 2020

Abstract

Objectives
The main aim of this work is to introduce a robust controller for controlling the drug dosage.

Methods
The presented work establishes a novel robust controller that controls the drug dosage and it also carried out parameters estimation. Along with this, a Regularized Error Function-based EKF (REF-EKF) is introduced for estimating the tumor cells that could be adapted for different conditions. It also assists in solving the overfitting problems, which occur during the drug dosage estimation. Moreover, the performance of the adopted controller is compared over other conventional schemes, and the attained outcomes reveal the appropriate impact of drug dosage injection on immune, normal, and tumor cells. It is also ensured that the presented controller does a robust performance on the parameter uncertainties. Moreover, to enhance the performance of the proposed system and for fast convergence, it is aimed to fine-tune the initial state of EKF optimally using a new Improved Gray Wolf Optimization (GWO) termed as Adaptive GWO (AGWO). Finally, analysis is held to validate the betterment of the presented model.

Results
The outcomes, the proposed method has accomplished a minimal value of error with an increase in time, when evaluated over the compared models.

Conclusions
Thus, the improvement of the proposed REF-EKF-AGWO model is proved from the attained results.
Semibinomial conditionally nonlinear autoregressive models of discrete random sequences: probabilistic properties and statistical parameter estimation
Authors: Voloshko Valeriy A. , Kharin Yuriy S. 
Published: December 2020

AbstractWe introduce a new model ${\mathscr{P}}$-CNAR(s) of sequences of discrete random variables with long memory determined by semibinomial conditionally nonlinear autoregression of order s ∈ ℕ with small number of parameters. Probabilistic properties of this model are studied. For parameters of the model ${\mathscr{P}}$-CNAR a family of consistent asymptotically normal statistical FB-estimates is suggested and the existence of an efficient FB-estimate is proved. Computational advantages of FB-estimate w.r.t. maximum likelihood estimate are shown: less restrictive sufficient conditions for uniqueness, explicit form of FB-estimate, fast recursive computation algorithm under extension of the model ${\mathscr{P}}$-CNAR. Subfamily of “sparse” FB-estimates that use some subset of frequencies of s-tuples is constructed, the asymptotic variance minimization problem within this subfamily is solved.
Historians, Public History, and Transitional Justice: Baltic Experiences
Authors: Pettai Eva-Clarita 
Published: December 2020

Abstract
How does public history correlate with issues of transitional justice and democratization? What are the roles and functions of professional historians during revolutionary moments and in the building of democratic culture and the pursuit of accountability and justice? On the basis of examples from three former Soviet republics, Estonia, Latvia and Lithuania during the past three decades, the essay reflects on these and other questions. It stresses the need for de-centralizing public engagement with the past and cautions against trends across the Central and Eastern European region to link processes of historical knowledge production with claims for justice and redress.
Blow-up of solutions for a Kirchhoff type equation with variable-exponent nonlinearities
Authors: Shahrouzi Mohammad , Kargarfard Firoozeh 
Published: December 2020

Abstract
This paper deals with a Kirchhoff type equation with variable exponent nonlinearities, subject to a nonlinear boundary condition. Under appropriate conditions and regarding arbitrary positive initial energy, it is proved that solutions blow up in a finite time. Moreover, we obtain the upper bound estimate of the blow-up time.
Metabelian groups: Full-rank presentations, randomness and Diophantine problems
Authors: Garreta Albert , Legarreta Leire , Miasnikov Alexei , Ovchinnikov Denis 
Published: December 2020

Abstract
We study metabelian groups 𝐺 given by full rank finite presentations

⟨
A
∣
R
⟩

M

\langle A\mid R\rangle_{\mathcal{M}}

in the variety ℳ of metabelian groups.
We prove that 𝐺 is a product of a free metabelian subgroup of rank

max
⁡

{
0
,

|
A
|

-

|
R
|

}

\max\{0,\lvert A\rvert-\lvert R\rvert\}

and a virtually abelian normal subgroup, and that if

|
R
|

≤

|
A
|

-
2

\lvert R\rvert\leq\lvert A\rvert-2

, then the Diophantine problem of 𝐺 is undecidable, while it is decidable if

|
R
|

≥

|
A
|

\lvert R\rvert\geq\lvert A\rvert

.
We further prove that if

|
R
|

≤

|
A
|

-
1

\lvert R\rvert\leq\lvert A\rvert-1

, then, in any direct decomposition of 𝐺, all factors, except one, are virtually abelian.
Since finite presentations have full rank asymptotically almost surely, metabelian groups finitely presented in the variety of metabelian groups satisfy all the aforementioned properties asymptotically almost surely.