bluuebunny/crossref_metadata_2025_split huggingface dataset:
Individual differences in bilingual experience modulate executive control network and performance: behavioral and structural neuroimaging evidence
Authors: Gallo Federico , Novitskiy Nikolay , Myachykov Andriy , Shtyrov Yury 
Published: September 2020

AbstractDual/multiple language use has been shown to affect cognition and its neural substrate, although the replicability of such findings varies, partially due to neglecting the role of interindividual variability in bilingual experience. To address this, we operationalized the main bilingual experience factors as continuous variables, investigating their effects on executive control performance and neural substrate deploying a Flanker task and structural magnetic resonance imaging. First, higher L2 proficiency predicted better executive performance. Second, neuroimaging results indicated that bilingualism-related neuroplasticity may peak at a certain stage of bilingual experience and eventually revert, possibly following functional specialization. Importantly, experienced bilinguals optimized behavioral performance independently of volumetric variations, suggesting a degree of performance gain even with lower GMV. Hence, the effects of bilingualism on cognition may evolve with experience, with improvements in functional efficiency eventually replacing structural changes. We conclude that individual differences in bilingual experience modulate cognitive and neural consequences of bilingualism.
Comparison of food and beverage products’ availability, variety, price and quality in German and US supermarkets
Authors: Stroebele-Benschop Nanette , Wolf Kerstin , Palmer Katharine , Kelley Casey J , Jilcott Pitts Stephanie B 
Published: September 2020

AbstractObjective:To assess availability, variety, price and quality of different food products in a convenience sample of supermarkets in Germany and the USA.Design:Cross-sectional study using an adapted version of the Bridging the Gap Food Store Observation Form.Setting:Information on availability, quality, price and variety of selected food products in eight German and seven US supermarkets (discount and full service) was obtained and compared by country.Results:A general tendency for lower prices of fruits and vegetables in Germany was observed, while produce quality and variety did not seem to differ between countries, with the exception of the variety of some vegetables such as tomatoes. Chips and cereals did not differ significantly in variety nor price. In both countries, high energy-dense foods were lower in energy costs than lower energy-dense foods.Conclusions:The influence of food prices and availability on consumption should be further explored, including the impact of country differences.
Factors influencing the recruitment and retention of registered nurses in adult community nursing services: an integrative literature review
Authors: Chamanga Edwin , Dyson Judith , Loke Jennifer , McKeown Eamonn 
Published: September 2020

AbstractBackground:Adult community nursing services are evolving around the world in response to government policies and changing patient demographics. Amidst these changes, recruitment and retention of community nursing staff are proving a challenge. An integrative literature review has identified multiple factors that influence nurse retention in adult community nursing with sparse information on recruitment factors. Although factors impacting retention of community nurses have been identified, their generalisability around the world is a challenge as they are context and co-dependent. Indicating the need for this area of study to be explored at a local level, as the same factors present with different findings globally.Aim:To establish factors influencing recruitment and retention of registered nurses in adult community nursing services.Design:Integrative literature review.Data sources:Four electronic databases were searched in August 2019 from January 2008 to December 2018: CINAHL Complete, Web of Science, MEDLINE and PROQUEST. Both qualitative and quantitative studies focusing on factors influencing community nursing recruitment and retention were included.Review methods:An integrative literature review methodology by Whittemore and Knafl (The integrative review: updated methodology. Journal of Advanced Nursing 52, 546–553) was followed, supported by Cochrane guidelines on data synthesis and analysis using a narrative synthesis method. The Center for Evidence-Based Management (CEBMa) critical appraisal tools were used for study quality assessment.Results:Ten papers met the study inclusion criteria. Data synthesis and analysis revealed individual and organisational factors influencing the retention of community nurses with the following three dominant themes: (1) work pressure, (2) working conditions and (3) lack of appreciation by managers.Conclusion:The review identified context-dependent factors that influence adult community nurses’ retention with limited generalisability. There is a lack of data on factors influencing recruitment into adult community nursing; further research is needed to explore factors affiliated to community nursing recruitment.
Evaluation of resistance to stemphylium blight in interspecific recombinant inbred lines derived from Lens culinaris × Lens ervoides
Authors: Adobor Stanley , Podder Rajib , Banniza Sabine , Vandenberg Albert 
Published: September 2020

AbstractCultivated lentil suffers yield loss from stemphylium blight, caused by Stemphylium botryosum Wallr. Identification of sources of stemphylium blight resistance and knowledge of the mode of inheritance of resistance are important for developing resistant cultivars. The interspecific recombinant inbred line (RIL) population developed from a cross between the moderately resistant parent Lens culinaris cv. ‘Eston’ and the resistant parent L. ervoides (Brign.) Grande accession IG 72815 was evaluated for stemphylium blight resistance under controlled conditions at the University of Saskatchewan, Saskatoon, Canada, and under field conditions at the Pulses Research Centre (PRC), Ishurdi, Bangladesh. We hypothesized that resistance from both parents will lead to transgressive segregation indicative of pyramiding of resistance genes from the same. However, no resistant transgressive segregants were observed in the RIL population. A large proportion (50%) of the RILs had disease severity levels similar to the resistant parent IG 72815 in experiments conducted under natural disease pressure in Bangladesh. Under controlled conditions in Saskatoon, 38% of RILs had resistance levels similar to IG 72815. Across all environments, 14 RILs consistently had resistance levels similar to IG 72815. The distribution of disease severity scores for all RILs indicated polygenic inheritance of stemphylium blight resistance in the population. RILs with consistent resistant reactions should prove useful for lentil improvement programmes. This will contribute to increasing the productivity of lentil crops in North America and the Indo-Gangetic region, which account for more than 68% of world lentil production.
Getting the Message? Choice, Self-Selection, and the Efficacy of Social Movement Arguments
Authors: Testa Paul F. , Williams Tarah , Britzman Kylee , Hibbing Matthew V. 
Published: September 2020

AbstractThe dynamics of choice and self-selection are central features of politics but absent from most experimental designs. We show how designs that incorporate choice, by allowing some subjects the option to receive or avoid treatment, can be extended by randomizing conditional on subjects’ treatment choices to answer further questions of interest while preserving statistical power. We apply this design to study how the gender of messengers for the #MeToo social movement conditions who receives the movement’s message and how they respond. Our results, from both convenience and nationally representative samples, suggest that #MeToo movement’s message reaches a wide audience with the intended effect. The potential for backlash in response to the message appears limited but more likely when this message is delivered by a woman.
Hypoxia induces an endometrial cancer stem-like cell phenotype via HIF-dependent demethylation of SOX2 mRNA
Authors: Chen Guofang , Liu Binya , Yin Shasha , Li Shuangdi , Guo Yu’e , Wang Mengfei , Wang Kai , Wan Xiaoping 
Published: September 2020

AbstractEndometrial cancer stem cells (ECSCs) are stem-like cells endowed with self-renewal and differentiation abilities, and these cells are essential for cancer progression in endometrial cancer (EC). As hallmarks of the tumour microenvironment (TME), hypoxia and hypoxia-inducing factors (HIFs) give rise to the dysregulation of tumour stemness genes, such as SOX2. Against this backdrop, we investigated the regulatory mechanisms regulated by HIFs and SOX2 in ECSCs during EC development. Here, ECSCs isolated from EC cell lines and tissues were found to express stemness genes (CD133 and aldehyde dehydrogenase, ALDH1) following the induction of their ECSC expansion. Notably, m6A methylation of RNA and HIF-1α/2α-dependent AlkB homologue 5 (ALKBH5) participate in the regulation of HIFs and SOX2 in EC, as confirmed by the observations that mRNA levels of m6A demethylases and ALKBH5 significantly increase under hypoxic conditions in ECSCs. Moreover, hypoxia and high ALKBH5 levels restore the stem-like state of differentiated ECSCs and increase the ECSC-like phenotype, whereas the knockdown of HIFs or ALKBH5 significantly reduces their tumour initiation capacity. In addition, our findings validate the role of ALKBH5 in promoting SOX2 transcription via mRNA demethylation, thereby maintaining the stem-like state and tumorigenicity potential of ECSCs. In conclusion, these observations demonstrate a critical role for m6A methylation-mediated regulation of the HIF-ALKBH5-SOX2 axis during ECSC expansion in hypoxic TMEs.
A single-cell polony method reveals low levels of infected Prochlorococcus in oligotrophic waters despite high cyanophage abundances
Authors: Mruwat Noor , Carlson Michael C G , Goldin Svetlana , Ribalet François , Kirzner Shay , Hulata Yotam , Beckett Stephen J , Shitrit Dror , Weitz Joshua S , Armbrust E Virginia , Lindell Debbie 
Published: September 2020

Abstract
Long-term stability of picocyanobacteria in the open oceans is maintained by a balance between synchronous division and death on daily timescales. Viruses are considered a major source of microbial mortality, however, current methods to measure infection have significant methodological limitations. Here we describe a method that pairs flow-cytometric sorting with a PCR-based polony technique to simultaneously screen thousands of taxonomically resolved individual cells for intracellular virus DNA, enabling sensitive, high-throughput, and direct quantification of infection by different virus lineages. Under controlled conditions with picocyanobacteria-cyanophage models, the method detected infection throughout the lytic cycle and discriminated between varying infection levels. In North Pacific subtropical surface waters, the method revealed that only a small percentage of Prochlorococcus (0.35–1.6%) were infected, predominantly by T4-like cyanophages, and that infection oscillated 2-fold in phase with the diel cycle. This corresponds to 0.35–4.8% of Prochlorococcus mortality daily. Cyanophages were 2–4-fold more abundant than Prochlorococcus, indicating that most encounters did not result in infection and suggesting infection is mitigated via host resistance, reduced phage infectivity and inefficient adsorption. This method will enable quantification of infection for key microbial taxa across oceanic regimes and will help determine the extent that viruses shape microbial communities and ecosystem level processes.
Intriguing size distribution of the uncultured and globally widespread marine non-cyanobacterial diazotroph Gamma-A
Authors: Cornejo-Castillo Francisco M , Zehr Jonathan P 
Published: September 2020

Abstract
Non-cyanobacterial diazotrophs (NCDs) have recently emerged as potentially important contributors to marine nitrogen fixation. One of the most widely distributed NCDs is Gamma-A, yet information about its autecology is still scarce and solely relies on the PCR-based detection of its nitrogenase (nifH) gene in seawater, since previous metagenomic surveys targeting free-living planktonic size fractions (&lt;3 μm) have not detected it. Here, we explore the diversity, biogeography, size-distribution, and nitrogenase gene expression of Gamma-A across four larger planktonic size-fractions (0.8–5, 5–20, 20–180, and 180–2000 μm) using metagenomes and metatranscriptomes from the Tara Oceans. We detected a single variant of a complete Gamma-A nifH gene along with other nitrogenase-related genes (nifKDT) within a metatranscriptomic-based contig of the Marine Atlas of Tara Ocean Unigenes. Gamma-A was detected in tropical and subtropical oceanic regions across all the size-fractions. However, the highest gene and transcript abundances were found in the 0.8–5 and 5–20 μm size-fractions at the surface, whereas abundances at the deep chlorophyll maximum were lower and similar across all size-fractions. The ubiquitous presence of active Gamma-A in large planktonic size-fractions suggests a filamentous or particle-attached lifestyle and places its potential to fix nitrogen in larger planktonic compartments.
Exosomal lncRNA DOCK9-AS2 derived from cancer stem cell-like cells activated Wnt/β-catenin pathway to aggravate stemness, proliferation, migration, and invasion in papillary thyroid carcinoma
Authors: Dai Wencheng , Jin Xiaoxia , Han Liang , Huang Haijing , Ji Zhenhua , Xu Xinjiang , Tang Mingming , Jiang Bin , Chen Weixian 
Published: September 2020

AbstractExosomal long non-coding RNAs (lncRNAs) are crucial factors that mediate the extracellular communication in tumor microenvironment. DOCK9 antisense RNA2 (DOCK9-AS2) is an exosomal lncRNA which has not been investigated in papillary thyroid carcinoma (PTC). Based on the result of differentially expressed lncRNAs in PTC via bioinformatics databases, we discovered that DOCK9-AS2 was upregulated in PTC, and presented elevation in plasma exosomes of PTC patients. Functionally, DOCK9-AS2 knockdown reduced proliferation, migration, invasion, epithelial-to-mesenchymal (EMT) and stemness in PTC cells. PTC-CSCs transmitted exosomal DOCK9-AS2 to improve stemness of PTC cells. Mechanistically, DOCK9-AS2 interacted with SP1 to induce catenin beta 1 (CTNNB1) transcription and sponged microRNA-1972 (miR-1972) to upregulate CTNNB1, thereby activating Wnt/β-catenin pathway in PTC cells. In conclusion, PTC-CSCs-derived exosomal lncRNA DOCK9-AS2 activated Wnt/β-catenin pathway to aggravate PTC progression, indicating that DOCK9-AS2 was a potential target for therapies in PTC.
LIN28B-AS1-IGF2BP1 binding promotes hepatocellular carcinoma cell progression
Authors: Zhang Jian , Hu Kewei , Yang Yong-qiang , Wang Yin , Zheng Yu-fan , Jin Yong , Li Ping , Cheng Long 
Published: September 2020

AbstractIGF2BP1 overexpression promotes hepatocellular carcinoma (HCC) progression. Long non-coding RNA LIN28B-AS1 directly binds to IGF2BP1. In the present study, LIN28B-AS1 and IGF2BP1 expression and their potential functions in HCC cells were tested. Genetic strategies were applied to interfere their expression, and cell survival, proliferation and apoptosis were analyzed. We show that LIN28B-AS1 is expressed in established/primary human HCC cells and HCC tissues. RNA-immunoprecipitation (RIP) and RNA pull-down results confirmed that LIN28B-AS1 directly associated with IGF2BP1 protein in HCC cells. LIN28B-AS1 silencing (by targeted siRNAs) or knockout (KO, by CRISPR-Cas9 method) depleted IGF2BP1-dependent mRNAs (IGF2, Gli1, and Myc), inhibiting HCC cell growth, proliferation, migration, and invasion. Conversely, ectopic overexpression of LIN28B-AS1 upregulated IGF2BP1-dependent mRNAs and promoted HCC cell progression in vitro. Importantly, ectopic IGF2BP1 overexpression failed to rescue LIN28B-AS1-KO HepG2 cells. LIN28B-AS1 siRNA and overexpression were ineffective in IGF2BP1-KO HepG2 cells. In vivo, LIN28B-AS1 KO-HepG2 xenograft tumors grew significantly slower than the control tumors in the nude mice. Taken together, we conclude that LIN28B-AS1 associates with IGF2BP1 to promote human HCC cell progression in vitro and in vivo.
Kidney GATA3+ regulatory T cells play roles in the convalescence stage after antibody-mediated renal injury
Authors: Sakai Ryota , Ito Minako , Komai Kyoko , Iizuka-Koga Mana , Matsuo Kazuhiko , Nakayama Takashi , Yoshie Osamu , Amano Koichi , Nishimasu Hiroshi , Nureki Osamu , Kubo Masato , Yoshimura Akihiko 
Published: September 2020

AbstractFoxP3+ regulatory T cells (Tregs) play crucial roles in peripheral immune tolerance. In addition, Tregs that reside or accumulate in nonlymphoid tissues, called tissue Tregs, exhibit tissue-specific functions and contribute to the maintenance of tissue homeostasis and repair. In an experimental mouse model of crescentic glomerulonephritis induced by an anti-glomerular basement membrane antibody, Tregs started to accumulate in the kidney on day 10 of disease onset and remained at high levels (~30–35% of CD4+ T cells) during the late stage (days 21–90), which correlated with stable disease control. Treg depletion on day 21 resulted in the relapse of renal dysfunction and an increase in Th1 cells, suggesting that Tregs are essential for disease control during the convalescence stage. The Tregs that accumulated in the kidney showed tissue Treg phenotypes, including high expression of GATA3, ST2 (the IL33 receptor subunit), amphiregulin (Areg), and PPARγ. Although T-bet+ Tregs and RORγt+ Tregs were observed in the kidney, GATA3+ Tregs were predominant during the convalescence stage, and a PPARγ agonist enhanced the accumulation of GATA3+ Tregs in the kidney. To understand the function of specific genes in kidney Tregs, we developed a novel T cell transfer system to T cell-deficient mice. This experiment demonstrates that ST2, Areg, and CCR4 in Tregs play important roles in the accumulation of GATA3+ Tregs in the kidney and in the amelioration of renal injury. Our data suggest that GATA3 is important for the recruitment of Tregs into the kidney, which is necessary for convalescence after renal tissue destruction.
Signature of local stress states in the deformation behavior of metallic glasses
Authors: Bian Xilei , Şopu Daniel , Wang Gang , Sun Baoan , Bednarčik Jozef , Gammer Christoph , Zhai Qijie , Eckert Jürgen 
Published: September 2020

AbstractThe design of ductile heterogeneous metallic glasses (MGs) with enhanced deformability by purposely controlling the shear-band dynamics via modulation of the atomic-scale structures and local stress states remains a significant challenge. Here, we correlate the changes in the local atomic structure when cooling to cryogenic temperature with the observed improved shear stability. The enhanced atomic-level structural and elastic heterogeneities related to the nonaffine thermal contraction of the short-range order (SRO) and medium-range order (MRO) change the characteristics of the activation process of the shear transformation zones (STZs). The experimental observations corroborated by Eshelby inclusion analysis and molecular dynamics simulations disclose the correlation between the structural fluctuations and the change in the stress field around the STZ. The variations in the inclination axes of the STZs alter their percolation mechanism, affect the shear-band dynamics and kinetics, and consequently delay shear failure. These results expand the understanding of the correlation between the atomic-level structure and elementary plastic events in monolithic MGs and thereby pave the way for the design of new ductile metallic alloys.
Methane emissions from natural gas vehicles in China
Authors: Da Pan  , Tao Lei , Sun Kang , Golston Levi M. , Miller David J. , Zhu Tong , Qin Yue , Zhang Yan , Mauzerall Denise L. , Zondlo Mark A. 
Published: September 2020

AbstractNatural gas vehicles (NGVs) have been promoted in China to mitigate air pollution, yet our measurements and analyses show that NGV growth in China may have significant negative impacts on climate change. We conducted real-world vehicle emission measurements in China and found high methane emissions from heavy-duty NGVs (90% higher than current emission limits). These emissions have been ignored in previous emission estimates, leading to biased results. Applying our observations to life-cycle analyses, we found that switching to NGVs from conventional vehicles in China has led to a net increase in greenhouse gas (GHG) emissions since 2000. With scenario analyses, we also show that the next decade will be critical for China to reverse the trend with the upcoming China VI standard for heavy-duty vehicles. Implementing and enforcing the China VI standard is challenging, and the method demonstrated here can provide critical information regarding the fleet-level CH4 emissions from NGVs.
Loss of MTX2 causes mandibuloacral dysplasia and links mitochondrial dysfunction to altered nuclear morphology
Authors: Elouej Sahar , Harhouri Karim , Le Mao Morgane , Baujat Genevieve , Nampoothiri Sheela , Kayserili Hϋlya , Menabawy Nihal Al , Selim Laila , Paneque Arianne Llamos , Kubisch Christian , Lessel Davor , Rubinsztajn Robert , Charar Chayki , Bartoli Catherine , Airault Coraline , Deleuze Jean-François , Rötig Agnes , Bauer Peter , Pereira Catarina , Loh Abigail , Escande-Beillard Nathalie , Muchir Antoine , Martino Lisa , Gruenbaum Yosef , Lee Song-Hua , Manivet Philippe , Lenaers Guy , Reversade Bruno , Lévy Nicolas , De Sandre-Giovannoli Annachiara 
Published: September 2020

AbstractMandibuloacral dysplasia syndromes are mainly due to recessiveLMNAorZMPSTE24mutations, with cardinal nuclear morphological abnormalities and dysfunction. We report five homozygous null mutations inMTX2, encoding Metaxin-2 (MTX2), an outer mitochondrial membrane protein, in patients presenting with a severe laminopathy-like mandibuloacral dysplasia characterized by growth retardation, bone resorption, arterial calcification, renal glomerulosclerosis and severe hypertension. Loss of MTX2 in patients’ primary fibroblasts leads to loss of Metaxin-1 (MTX1) and mitochondrial dysfunction, including network fragmentation and oxidative phosphorylation impairment. Furthermore, patients’ fibroblasts are resistant to induced apoptosis, leading to increased cell senescence and mitophagy and reduced proliferation. Interestingly, secondary nuclear morphological defects are observed in bothMTX2-mutant fibroblasts and mtx-2-depletedC. elegans. We thus report the identification of a severe premature aging syndrome revealing an unsuspected link between mitochondrial composition and function and nuclear morphology, establishing a pathophysiological link with premature aging laminopathies and likely explaining common clinical features.