bluuebunny/crossref_metadata_2025_split huggingface dataset:
Characteristics of Socially High-risk Pregnant Women in Telephone Triage
Authors: Ohkawa Kana , Ishikawa Momoko , Yoshida Hikaru , Ito Hijiri , Sagawa Tadashi 
Published: May 2020

Abstract
BackgroundTo clarify the frequency, co-occurrence of relationships, and characteristic words of socially high-risk pregnant women who contacted the night telephone triage for advice on pregnancy.MethodsWe performed a quantitative study involving 5,548 of 13,496 pregnancy cases recorded by telephone triage. The contents of telephone triage conversations were examined to determine whether they fulfilled the requirements for each category of socially high-risk pregnant women. The frequency, co-occurrence of relationships, and characteristic words of each category ofsocially high-risk pregnant women were analyzed.ResultsThere were 432 socially high-risk pregnant women. Among 15 categories of socially high-risk pregnant women, young pregnant women (183 cases), mental illness (88 cases), and no antenatal care (80 cases) were the top three categories. There was a strong co-occurring relationship between the following categories of socially high-risk pregnant women: out of touch and lack of supporters; no antenatal care and money matters; out of touch and unmarried; no antenatal care and no issue; money matters and lack of supporters; no issue and lack of supporters; and money matters and unmarried. In characteristic words, some words did not overlap with the top 10 words in other categories.ConclusionsA telephone triage that allows pregnant women to freely talk about their concerns with a coordinator might be useful for detecting socially high-risk pregnant women. To efficiently identify socially high-risk pregnant women, it might be necessary to understand the characteristic words of each category and subsequently determine if the client fulfills the criteria for more than one category of socially high-risk pregnant women.
Comparison of complete renal response and mortality in early- and late-onset lupus nephritis: A multicenter retrospective study of a Japanese cohort
Authors: Ichinose Kunihiro , Kitamura Mineaki , Sato Shuntaro , Fujikawa Keita , Horai Yoshiro , Matsuoka Naoki , Tsuboi Masahiko , Nonaka Fumiaki , Shimizu Toshimasa , Sumiyoshi Remi , Koga Tomohiro , Kawashiri Shin-ya , Iwamoto Naoki , Igawa Takashi , Tamai Mami , Nakamura Hideki , Origuchi Tomoki , Nishino Tomoya , Kawakami Atsushi 
Published: May 2020

Abstract
Background
Most patients with systemic lupus erythematosus (SLE) progress to lupus nephritis (LN) within 5 years of their SLE diagnosis, although it is not uncommon for LN to develop at later times. Here we evaluated the clinical features of early- and late-onset LN.
Patients and Methods:
We retrospectively analyzed the cases of a total of 184 of the 201 patients who underwent a renal biopsy at Nagasaki University Hospital and community hospitals between 1990 and 2016 and were diagnosed as having LN. Early-onset was defined as the development of LN within the first 5 years after the patient's SLE diagnosis, and late-onset was defined as LN development > 5 years post-diagnosis. We analyzed the complete renal response (CR) at 6 and 12 months after induction therapy, the classification of renal pathology, and the mortality of the early- and late-onset LN groups.
Results
The mean follow-up duration after renal biopsy was 123 ± 85 months. There were 113 (61.4%) early-onset patients and 71 (38.6%) late-onset patients. A multivariate analysis revealed that the following factors were predictive of CR. At 6 months: female gender (odds ratio [OR] 4.64, 95% confidence interval [CI] 1.66–12.98, p = 0.002), proteinuria (OR 0.82, 95%CI 0.70–0.95, p = 0.003), index of activity (0–24) (OR 0.85, 95%CI 0.74–0.97, p = 0.014), and early-onset LN (OR 2.26, 95%CI 1.11–4.58, p = 0.023). At 12 months: female gender (OR 3.95, 95% CI 1.62–9.64, p = 0.002), index of activity (0–24) (OR 0.80, 95%CI 0.71–0.91, p < 0.001), and early-onset LN (OR 1.98, 95%CI 1.04–3.79, p = 0.038). In a Cox proportional hazards model, the early-onset LN group had a significantly better mortality rate than the late-onset LN group (p = 0.038).
Conclusions
In our cohort, early-onset LN was a better predictor of 6- and 12-month of CR attainment. Our results suggest that early-onset LN patients had better mortality than late-onset LN patients.
Laboratory features in severe vs. non-severe COVID-19 patients, a systematic review and meta-analysis
Authors: Ghahramani Sulmaz , Tabrizi Reza , Lankarani Kamran B , Kashani Seyyed mohammad amin , Rezaei Shahla , Zeidi Nazanin , Akbari Maryam , Heydari Seyed Taghi , Akbari Hamed , Nowrouzi-sohrabi Peyman , Ahmadizar Fariba 
Published: May 2020

Abstract
OBJECTIVE: Understanding the common laboratory features of COVID-19 in severe cases versus non-severe patients could be quite useful for clinicians and might help to predict the model of disease progression. MATERIALS AND METHODS: Electronic databases were systematically searched in PubMed, EMBASE, Scopus, Web of ‎Science, and Google Scholar from inception to 3rd of March 2020. Heterogeneity across included ‎studies was determined using Cochrane’s Q test and the I2 statistic. We used the fixed or random-effect models to pool ‎the weighted mean differences (WMDs) or standardized mean differences and 95% confidence ‎intervals (CIs).‎RESULTS:‎ Out of a total of 3009 citations, 17 articles (22 studies, 21 from China and one study from Singapour) with 3396 ranging from 12-1099 patients, ‎were included. Our meta-analyses showed a significant decrease in ‎lymphocyte, monocyte, and eosinophil, hemoglobin, platelet, albumin, serum sodium, lymphocyte to C-reactive protein ratio (LCR), leukocyte to C-reactive protein ratio (LeCR), leukocyte to IL-6 ratio (LeIR), and an increase in the ‎neutrophil, alanine ‎aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin, blood urea nitrogen (BUN), creatinine (Cr), erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), procalcitonin (PCT), lactate dehydrogenase (LDH), fibrinogen, prothrombin time (PT), D-dimer, glucose ‎level, and neutrophil to lymphocyte ratio (NLR) in the severe group compared with the non-severe group. However, no significant changes were observed in white blood cells (WBC), ‎ creatine kinase (CK), troponin I, myoglobin, interleukin-6 (IL-6), and potassium (K) between the two groups.‎CONCLUSIONS: This meta-analysis provides evidence for the differentiation of severe cases of COVID-19 based on laboratory test results at the time of hospital admission. Future well-methodologically designed studies from other populations are strongly recommended.
On the pool boiling heat transfer enhancement of distilled water using Cu-nanoparticles textured with carbon nanofibers
Authors: Ali Mohamed R. O. , Barakat Nasser A. M. , Bassiouny Ramadan , Moghazy Ibrahim M. M. El , Ragab Mahmoud M. S. 
Published: May 2020

Abstract
Enhancement of subcooled and saturation pool boiling is of interest for many applications. The present study proposes coating heated surface using nanotextured of Cu nanoparticles with carbon nanofibers. This texture formation on copper substrate surface passes through different chemical stages under specific conditions. Using electrospinning, coating was carried out at different times (5, 15, and 30 min). After vacuum drying at 60 oC for 24 h, the copper substrates attaching the electrospun mat were calcined at 850 oC under vacuum. and accordingly, different contact angles were measured as 80o, 115o, and 102o. The coated surfaces are coded by S1, S2 and S3 for the electrospun coated surfaces of (5, 15, and 30), respectively.All nanostructured coated surfaces showed a good heat transfer enhancement compared to uncoated copper surface for both HTC and CHF. The results showed an enhancement of HTC by 57.83%, 40.3% for S2 and S3 respectively and an enhancement of CHF by 13.67%, 17.11% and 48.14% for S1, S2 and S3 respectively.
Engineered chromosome-based T7 RNA polymerase in Escherichia coli W3110 for orthogonal T7 promoter circuit as a cell factory
Authors: Ting Wan-Wen , Tan Shih-I , Ng I-Son 
Published: May 2020

Abstract
Background

Orthogonal T7 RNA polymerase (T7RNAP) and T7 promoter were powerful tools to mediate the protein expression. Moreover, Escherichia coli W3110 strain possesses more advantages than the B strain due to more heat shock proteins and higher tolerance to chemicals. Therefore, implementation of T7-based system in W3110 strain is a conceivable strategy to develop the cell factory.
Results

Three novel W3110 strains with chromosome-equipped T7RNAP (i.e W3110:IL5, W3110::L5 and W3110::pI) were engineered to demonstrate the feasibility on protein expression and chemical production. At first, the LacZ and T7RNAP with IPTG induction showed higher expression levels in W3110 derivatives than that in BL21(DE3). The plasmids with and without lacI/lacO repression were used to investigate the protein expression of super-fold green fluorescence protein (sfGFP), Cas9, carbonic anhydrase (CA) and lysine decarboxylase (CadA). All the proteins were expressed higher and enzymatic functions were better in W3110::L5 and W3110::pI. Moreover, the highest cadaverine production, lysine consumption and the yield were obtained in W3110::L5(+) strain with pET28a(+)-CadA which reached 32.2 g/L, 45 g/L and 91.7% at 24 h, while the W3110::pI(-) strain with pSU-T7-CadA achieved 36.9 g/L, 43.8 g/L and 103.4% at 12 h which is unnecessary of inducer.
Conclusion

Inducer and lacI/lacO regulators on chromosome and plasmid have been investigated in W3110 strains with T7RNAP. The newly engineered W3110::L5 and W3110:pI both possessed similar protein expression compared to commercial BL21(DE3). Furthermore, among all strains, W3110::pI displayed the greatest potential as cell factory in the future.
Capicua homology protein inhibits the progression of gastric cancer through the PI3K/AKT signaling pathway
Authors: GE LU , Hu Chang-long , Ge Zheng-hui , Wang Chun-rong , Qian Li , Hu Jiang-hong , Zhang Jian-guo 
Published: May 2020

Abstract
Purpose

Capicua homolog protein (CIC) played a broad role in the development of cancer in humans, however, its role in the progression of gastric cancer (GC) specifically has been unclear. This study aimed to explore the expression of CIC and its potential clinical value in patients with GC.

Methods

The CIC levels in GC tissues and cell lines were examined by quantitative real-time polymerase chain reaction (qRT-PCR). And the in-vitro effects of CIC expression in MGC-803 cells on their proliferation, invasion, and the progression of epithelial-mesenchymal transition were assessed by CCK-8 assays, Matrigel-invasion analysis, qRT-PCR and Western blot assays, separately. In addition, the effects of downregulation of CIC on the activation of PI3K/AKT signaling pathway were measured using Western-blot analysis.

Results

The results showed CIC levels were lower in GC tissues and GC cell lines, and these lower CIC levels were correlated with tumor differentiation, Helicobacter pylori infection, TNM stage, and patient survival. In addition, CIC overexpression could promote cell proliferation, invasion, and progression of epithelial-mesenchymal transition in MGC-803 cells. Notably, exotic expression of CIC inactivated the phosphoinositide 3-kinase/protein kinase B signaling pathway.

Conclusions

In conclusion, our finding suggested CIC could serve as a potential diagnostic and prognostic biomarker and a probable therapy target for GC.
Circ_0000190 favors gastric cancer patients potentially via inhibiting miR-1252/PAK3 pathway
Authors: Wang Gui Jun , Yu Tian Yu , Li Yan Rong , Liu Yang Jun , Deng Bei-Bei 
Published: May 2020

Abstract
Background Gastric cancer accounts for 8.3% of all cancer death and is demonstrated associated with aberrant circular RNAs (circRNAs) expressions.Circ_0000190 has been noted with prognostic role in gastric cancer. We aim to investigate the role and the underlying mechanism of circ_0000190 in gastric cancer.Methods Circ_0000190 expressions were examined in gastric cancer and adjacent normal tissues by RT-PCR. With gastric cancer cell lines, circ_0000190 expression was detected by FISH and RT-PCR. After forced expression, the role of the circRNA in gastric cancer cell viability, apoptosis, proliferation, cell cycle and migration was observed. The potential effector of circ_0000190 was predicted by computational screen and validated by luciferase reporter assay. The association of the effectors with circ_0000190’s effects above mentioned were examined. Mice model of human gastric cancer was established to observe the underlying mechanisms of circ_0000190.Results Circ_0000190 was down-regulated in gastric cancer tissue and cells, with a major location in cytoplasm. After transfection, circ_0000190 inhibited gastric cancer cell viability, proliferation and migration, and induced apoptosis and cycle, which was correlated with increased capase-3 and p27 expression, and decreased cyclinD expression. The circRNA was validated as a sponge of miR-1252, which directly targeted PAK3. The effects by circ_0000190 on the cellular processes above mentioned were blocked by miR-1252 mimics, and this was rescued after further overexpression of PAK3.Conclusions Our results revealed that circ_0000190 protected against gastric cancer, and this was via directly targeting miR-767-5p/PAK3 axis. Therefore, employing circ_0000190 might be a promising therapeutic strategy for treatment of gastric cancer.
Experimental Investigation of the Vortex Flow around an Axisymmetric Body with the Five-Hole Probe and Hot Wire Probe
Authors: farajollahi amir , Manshadi Mojtaba Dehghan , Hejranfar Kazem 
Published: May 2020

Abstract
Anaxisymmetric body experiences the vertical flow around itself at incidence angle. If the adverse pressure gradient is significant, the boundary layers separated and a vortex is formed. The flow over a submarine at AOA (angle of attack) has specified separation of boundary layer and large vortex structures around the body. This flow influences body drag, acoustic and maneuverability. A propermethod to decrease and control the impacts of this separated flowis to use vortex generator. The mainobjective of the present study is to investigate the flow field around a Suboff model with applying the vortex generator by using the hot wire and five-hole probe in 0° ≤ α ≤ 20° angles of attack. The novelty of present study is application of two experimental method, (hot wire probe and five-hole probe) which can help to precisely study the structure of three-dimensional vortical flow field, the boundary layer velocity profiles and probability of the separation on the model with and without existence of vortex generator. The results indicate that vortex generators significantly decrease cross-flow separation, the size of vortices and the vortical flow.
Can Obesity Prevalence&nbsp;Explain COVID-19 Indicators (Cases, Mortality and Recovery)? A Comparative Study in OECD Countries
Authors: Arbel Yuval , Fialkoff Chaim , Kerner Amichai , Kerner Miryam 
Published: May 2020

Abstract
Coronavirus disease 2019 (COVID-19) is a declared global pandemic with multiple risk factors. Obesity is considered by several researchers as one of the serious risk factors for coronavirus complications based on recent empirical studies. Yet, other scholars argue in favor of the existence of an obesity survival paradox, and criticize the former group of studies on the grounds that they lack controls for race, socioeconomic status, or quality of care. The objective of the current study is to analyze the potential relationships between different corona indicators and obesity on a state-wide level, based on an OECD report. In an attempt to test the counter-intuitive possibility of an obesity survival paradox, the proposed empirical model relaxes the assumption of monotonic change by applying the quadratic design and testing which one of the two competing models (i.e., quadratic or linear) better fits the data. Findings suggest more complex relationships between corona indices and obesity rates than previously thought. Consequently, ethical guidelines referring to priority in intubation and intensive care treatments should account for these complex relationships between obesity and corona. Indeed, there is a linear increase of mortality rate from corona with elevated prevalence of obesity. Yet, other indicators, such as the number of infected per 100,000 persons, rates of severe corona cases, rates of recovered corona patients and corona as the cause of death exhibit quadratic, rather than linear, patterns. The reasons for these non-linear patterns might be explained by several conditions such as increased metabolic reserves, more aggressive treatment, other non-corona complications for obese persons and unidentified factors that should be examined in future research.
Molecular characterization of extended-spectrum β-lactamase-producing Escherichia coli and Klebsiella pneumoniae among the pediatric population in Qatar
Authors: Perez-Lopez Andres , Sundararaju Sathyavathi , Al-Mana Hassan , Tsui Kin Ming , Hasan Mohammad , Tang Patrick 
Published: May 2020

Abstract
Background: Although extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae are a public health problem in the Arabian Peninsula, data on the molecular characteristic of their antimicrobial resistance determinants in children is limited.Methods: Whole-genome sequencing (WGS) was performed on ESBL-producing E. coli and K. pneumoniae isolates recovered from screening and clinical specimens from pediatric patients at Sidra Medicine in Doha from January to December 2018.Results: WGS was performed on 327 ESBL producers: 255 E. coli and 72 K. pneumoniae. The most common sequence types (ST) were ST131 (16.5%), ST38 and ST10 (8.2 each%) in E. coli and ST307 (9.7%), ST45 and ST268 (6.9% each) in K. pneumoniae. CTX-M type ESBL were found in all but one isolate, with CTX-M-15 accounting for 87.8%. Co-carriage of OXA-1 alone or in combination with TEM-1B was associated with reduced susceptibility toamoxicillin/clavulanate (P=0.002 and P<0.0001) and piperacillin/tazobactam (P=0.02 and P=0.004). The most common plasmid-mediated quinolone resistance genes co-carried were qnr A/B/E/S (45.3%). Ninety percent of gentamicin non-susceptible isolates harboured genes encoding AAC(3) enzymes, mainly aac(3)-IIa. Only 2 of 57 isolates harbouring aac(6’)-Ib-cr were non-susceptible to amikacin. Eighty-five percent of isolates carried IncF plasmids. Conclusions: Our data show that CTX-M is largely the most prevalent ESBL type in the pediatric population in Qatar with a predominance of CTX-M-15. Carbapenem-sparing options to treat ESBL infections are limited given the frequent co-production of OXA-1 and TEM-1B enzymes and co-resistance to antibiotic classes other than β-lactams.
Pattern Analysis of Continuous Analytic Wavelet Transforms of the COVID19 Spreading and Death
Authors: Wang Yanshuo 
Published: May 2020

Abstract

Wavelet transform is frequently used for time series data to extract the frequency localization information in last two decades. The wavelet transform with Morse wavelet is applied to the daily infected cases and deaths of COVID19 at different countries/places. There are multiple scales (frequencies) of COVID19 spreading with different magnitude at the specific time which can be identified in Wavelet magnitude scalogram plots. For example, in China, the highest magnitude (2000 to 2500) spreading was happened on 2/12/202 with scale around 329 to 434, but in U.S.A the highest magnitude (4000 to 4500) spreading was happened during 4/25/2020 and 4/28/2020 with scale 137 to 183. The summary of the wavelet magnitude and scale at specific period for different countries/places is presented in this paper
On two cases of atypical respiratory distress in eastern Democratic Republic of the Congo months before the COVID-19 pandemic was declared. Could SARS-Cov-2 have been already spreading? Case report
Authors: MATESO Guy-Quesney , BAGUMA Marius , MWENE-BATU Pacifique , BALEMBA Ghislain MAHESHE , NZABARA Fabrice , MAKALI Samuel , BEDHA Aline , FURAHA Bonheur , MINANI Jimmy , MUHINDO Christian TSHONGO , MALEMBAKA Espoir BWENGE , MASIMANGO Mannix Imani , SHINDANO Tony Akilimali , CIKOMOLA Justin Cirhuza , MUBAGWA Kanigula 
Published: May 2020

Abstract
Background: Predictions have been made that Africa would be the most vulnerable continent to the novel coronavirus disease 2019 (COVID-19). Interestingly, the spread of the disease in Africa seems to be slower than in many parts of the World. Among possible hypotheses, some parts of Africa may have undergone a “silent” COVID-19 epidemic and acquired a herd immunity before the official declaration of the disease in December 2019.Case presentation: We report two cases (one 55-year-old man and one 25-year-old woman) of acute respiratory distress secondary to atypical pneumonia in Bukavu, in eastern Democratic Republic of the Congo (DRC) which occurred between September and December 2019, before the official beginning of the COVID-19 pandemic. One patient had returned from China and the other had close contacts with travellers from China in the 2 weeks prior to the onset of symptoms. In either case, the aetiology could not be accurately determined. However, the two cases presented a clinical picture (progressive dyspnoea, preceded by dry cough and fever) and laboratory changes (procalcitonin within the normal range, slight inflammation, and lymphopenia) compatible with a viral infection. The chest X-Rays series of the first patient showed lesions (reticulations, ground glass, and nodules ≤ 6 mm) similar to those found in COVID-19. In addition, unlike the 25-year-old female patient who had no comorbidity, the 55-year-old male patient who had hypertension as comorbidity, developed a more severe acute respiratory distress which progressed to death.Conclusion: These cases bring to the attention a number of facts which make us suspect that the COVID-19 epidemic may have already been present in the region months before the official beginning of the pandemic.
Clinical and Molecular Characteristics of 69 Chinese Patients with Ornithine Transcarbamylase Deficiency
Authors: Lu Deyun , Han Feng , Qiu Wenjuan , Zhang Huiwen , Ye Jun , Liang Lili , Wang Yu , Ji Wenjun , Zhan Xia , Gu Xuefan , Han Lianshu 
Published: May 2020

Abstract
Background: This study aimed to describe the clinical and biochemical features of Chinese patients with ornithine transcarbamylase deficiency (OTCD), and to investigate the mutation spectrum of OTC gene and their potential correlation with phenotype.Methods: Sixty-nine patients with OTCD were enrolled between 2004 and 2019. Clinical and laboratory data were reviewed retrospectively from medical records. Results: Fifteen cases (13 males, 2 females) presented with early onset; 53 cases (21 males, 32 females) had late onset, and one female was asymptomatic. The median onset age was 1.5 years (range, 1 day-56 years). Urine orotic acid levels were increased in all patients tested, while only 47.6% of patients showed decreased serum levels of citrulline. The peak plasma ammonia levels were higher in early-onset patients than in late-onset patients (P < 0.01). Fifty-four different mutations of OTC gene were identified and 18 of them were novel. R277W (10.6%) was the most common mutation, followed by G195R (4.6%) and A209V (3.0%). By June 2019, 41 patients had survived, 24 were deceased, and 4 were lost to follow-up. Among the survivors, 13 patients had received liver transplantation at a median age of 3 years, with a one-year survival rate of 100%. The mortality of OTCD is extremely high among patients with early onset (80.0% versus 24.5% in patients with late onset).Conclusions: The evaluation of serum citrulline level is of limited value in diagnosis of OTCD, while urine orotic acid detection and genetic testing are more helpful.