bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genomic Landscape of Uterine Sarcomas Defined Through Prospective Clinical Sequencing
Authors: Hensley Martee L. , Chavan Shweta S. , Solit David B. , Murali Rajmohan , Soslow Robert , Chiang Sarah , Jungbluth Achim A. , Bandlamudi Chaitanya , Srinivasan Preethi , Tap William D. , Rosenbaum Evan , Taylor Barry S. , Donoghue Mark T.A. , Hyman David M. 
Published: April 2020

Abstract

Purpose:
We examined whether prospective molecular characterization of advanced metastatic disease can reveal grade and/or histology-specific differences to inform diagnosis and facilitate enrollment onto clinical trials.

Experimental Design:
Patients with uterine sarcoma consented to a prospective study of next-generation sequencing (NGS). Clinical annotations were extracted from their medical record. Tumor and matched normal DNA were subjected to NGS, and the genomic landscape was explored for survival correlations and therapeutic targetability.

Results:
Tumors from 107 women were sequenced and included leiomyosarcoma (n = 80), high-grade non-leiomyosarcoma (n = 22), low-grade endometrial stromal sarcoma (LG-ESS, n = 4), and smooth muscle tumor of uncertain malignant potential (STUMP, n = 2). Genomic profiling influenced histologic diagnosis in three cases. Common uterine leiomyosarcoma alterations were loss-of-function mutations in TP53 (56%), RB1 (51%), and ATRX (31%). Homozygous deletions of BRCA2 were present in 5% of these patients. PTEN alteration frequency was higher in the metastases samples as compared with the primary samples. Genomes of low-grade tumors were largely silent, while 50.5% of high-grade tumors had whole-genome duplication. Two metastatic uterine leiomyosarcoma cases were hypermutated. Both had prolonged disease-free survival. Potentially actionable mutations were identified in 48 patients (45%), 8 (17%) of whom received matched therapy with 2 achieving clinical responses. Among patients with uterine leiomyosarcoma with somatic BRCA2 alterations, sustained partial responses were observed with PARP inhibitor–containing therapy.

Discussion:
Prospective genomic profiling can contribute to diagnostic precision and inform treatment selection in patients with uterine sarcomas. There was evidence of clinical benefit in patients with uterine leiomyosarcoma with somatic BRCA2 alterations treated with PARP inhibitors.
Galectin-3 Identifies a Subset of Macrophages With a Potential Beneficial Role in Atherosclerosis
Authors: Di Gregoli Karina , Somerville Michelle , Bianco Rosaria , Thomas Anita C. , Frankow Aleksandra , Newby Andrew C. , George Sarah J. , Jackson Christopher L. , Johnson Jason L. 
Published: April 2020

Objective:

Galectin-3 (formerly known as Mac-2), encoded by the
LGALS3
gene, is proposed to regulate macrophage adhesion, chemotaxis, and apoptosis. We investigated the role of galectin-3 in determining the inflammatory profile of macrophages and composition of atherosclerotic plaques.

Approach and Results:

We observed increased accumulation of galectin-3–negative macrophages within advanced human, rabbit, and mouse plaques compared with early lesions. Interestingly, statin treatment reduced galectin-3–negative macrophage accrual in advanced plaques within hypercholesterolemic (apolipoprotein E deficient)
Apoe
−/−
mice. Accordingly, compared with
Lgals3
+/+
:
Apoe
−/−
mice,
Lgals3
−/−
:
Apoe
−/−
mice displayed altered plaque composition through increased macrophage:smooth muscle cell ratio, reduced collagen content, and increased necrotic core area, characteristics of advanced plaques in humans. Additionally, macrophages from
Lgals3
−/−
mice exhibited increased invasive capacity in vitro and in vivo. Furthermore, loss of galectin-3 in vitro and in vivo was associated with increased expression of proinflammatory genes including MMP (matrix metalloproteinase)-12, CCL2 (chemokine [C-C motif] ligand 2), PTGS2 (prostaglandin-endoperoxide synthase 2), and IL (interleukin)-6, alongside reduced TGF (transforming growth factor)-β1 expression and consequent SMAD signaling. Moreover, we found that MMP12 cleaves macrophage cell-surface galectin-3 resulting in the appearance of a 22-kDa fragment, whereas plasma levels of galectin-3 were reduced in
Mmp12
−/−
:
Apoe
−/−
mice, highlighting a novel mechanism where MMP12-dependent cleavage of galectin-3 promotes proinflammatory macrophage polarization. Moreover, galectin-3–positive macrophages were more abundant within plaques of
Mmp12
−/−
:
Apoe
−/−
mice compared with
Mmp12
+/+
:
Apoe
−/−
animals.

Conclusions:
This study reveals a prominent protective role for galectin-3 in regulating macrophage polarization and invasive capacity and, therefore, delaying plaque progression.
Pervasive White Matter Fiber Degeneration in Ischemic Stroke
Authors: Egorova Natalia , Dhollander Thijs , Khlif Mohamed Salah , Khan Wasim , Werden Emilio , Brodtmann Amy 
Published: April 2020

Background and Purpose—
We examined if ischemic stroke is associated with white matter degeneration predominantly confined to the ipsi-lesional tracts or with widespread bilateral axonal loss independent of lesion laterality.

Methods—
We applied a novel fixel-based analysis, sensitive to fiber tract–specific differences within a voxel, to assess axonal loss in stroke (N=104, 32 women) compared to control participants (N=40, 15 women) across the whole brain. We studied microstructural differences in fiber density and macrostructural (morphological) changes in fiber cross-section.

Results—
In participants with stroke, we observed significantly lower fiber density and cross-section in areas adjacent, or connected, to the lesions (eg, ipsi-lesional corticospinal tract). In addition, the changes extended beyond directly connected tracts, independent of the lesion laterality (eg, corpus callosum, bilateral inferior fronto-occipital fasciculus, right superior longitudinal fasciculus).

Conclusions—
We conclude that ischemic stroke is associated with extensive neurodegeneration that significantly affects white matter integrity across the whole brain. These findings expand our understanding of the mechanisms of brain volume loss and delayed cognitive decline in stroke.
Bodily Injuries and Dative Experiencers in Old Frisian
Authors: Bruno Laura , Kerkhof Peter Alexander 
Published: April 2020

Abstract
This article offers a descriptive account of body part constructions in Old East Frisian texts and analyzes the occurrence of dative experiencers in such clauses. This includes a comparison between Old Frisian body part grammar and its Middle Dutch counterpart, revisiting issues such as the antiquity of dative external possessors and oblique subject constructions in West Germanic. In presenting the data from a theory-neutral perspective, this investigation contributes to the study of body part grammar in Medieval Germanic in particular and to the growing body of literature on Old Frisian syntax in general.
A review of neococcid scale insects (Hemiptera: Sternorrhyncha: Coccomorpha) based on the morphology of the adult males
Authors: HODGSON CHRIS 
Published: April 2020

The importance of adult male morphology in elucidating the phylogeny of scale insects (Coccomorpha) was first suggested by Balachowsky and Ferris in the late 1930s. However, the first extensive comparisons of adult male morphology were made by Jancke (1955) and Theron (1958), both of whom looked at the morphology of various groups of Coccomorpha. Theron’s study, under the guidance of K.L. Boratyński, set a new standard for detail and accuracy (although he did not describe the setae). These studies were closely followed by Beardsley (1962), who described the adult males of 30 mealybug species from Hawaii, and then by three more of Boratyński’s students who produced highly significant monographs on particular families, namely Ghauri (1962) who described 26 species of Diaspididae, Giliomee (1967a) who described 22 species of Coccidae and Afifi (1968) who described 17 species of Pseudococcidae and 7 species of Eriococcidae. Since then, the adult males of more than 300 neococcoid species have been described in considerable detail (Appendix A). Adult males of a further 48 species are described or redescribed and illustrated in this monograph (Acanthococcus adenostomae (Ehrhorn), Eriochiton armatus Brittin; Apiomorpha munita tereticornuta Gullan; A. ovicola (Froggatt); A. pharetrata (Schrader); A. rosaeformis (Froggatt); A. spinifer Froggatt; Dactylopius coccus (Costa); Callococcus leptospermi (Maskell); Lachnodius ?eucalypti (Maskell); Tanyscelis verrucula (Froggatt); Beesonia dipterocarpi Green; Parastictococcus brachystegiae (Hall); P. hargreavesi (Vayssière); P. multispinosus (Newstead); Stictococcus intermedius Newstead; S. vayssierei Richard; Conchaspis angraeci Cockerell; C. capensis Linnaeus; C. socialis Green; C. vayssierei Mamet; Leucaspis gigas (Maskell); Labidaspis myersi (Green); Allokermes galliformis (Riley); Kermes shastensis Ehrhorn; Kermes sp.; Tachardina aurantiaca (Cockerell); Tachardiella sp.; Cerococcus artemisiae (Cockerell); Antecerococcus indicus (Maskell); A. ornatus (Green); Bambusaspis delicata (Green); B. longa (Green); Asterolecanium petrophilae (Fuller); Hsuia cheni Borchsenius; Aclerda arundinariae McConnell; A. distorta Green; A. tillandsiae Howell; A. tokionis Cockerell; Aclerda sp. A; Aclerda sp. B; Luzulaspis caricis (Ehrhorn); Akermes scrobiculatus (Maskell); A. pingue (Maskell); Cardiococcus major (Maskell); Ctenochiton serratus Green; C. eucalypti Maskell, and ?Pulvinaria dodonaeae Maskell). This paper summarises the data from all of these descriptions, and provides diagnoses for the adult male morphology for all of the neococcoid families and other taxa discussed here. Because our concept of the “Eriococcidae” remains uncertain, extra attention has been payed to the taxa considered to be most closely involved. At least 1 illustration is included of an adult male of each of the taxa (mainly families) discussed here. Identification keys are provided for most of the males described to-date.
Machines, Monsters, and Coffin Corners: Broadcast Meteorologists’ Use of Figurative and Intense Language during Hurricane Harvey
Authors: Prestley Robert , Olson Michele K. , Vos Sarah C. , Sutton Jeannette 
Published: April 2020

AbstractDuring impactful weather events, television broadcast meteorologists act as high-profile risk communicators by interpreting complex weather information for viewers. However, the specific language choices broadcast meteorologists make in these situations have rarely been studied. This qualitative study investigates how meteorologists used figurative and emotionally intense language to interpret threats and impacts during Hurricane Harvey in August 2017, using 24 h of live broadcasts from KHOU, a Houston CBS affiliate. The results of this inductive, thematic analysis suggest that KHOU meteorologists framed Harvey as an all-encompassing monster and as a heat-seeking machine. The meteorologists also relied on emotionally intense language to emphasize Harvey’s extreme threat and to express their concern and disbelief about Harvey’s impact. This study indicates the specific and multifaceted ways broadcast meteorologists communicate risk and science during high-impact events and identifies key questions for future research.
Subannual to Interannual Variabilities of SST in the North Atlantic Ocean
Authors: Li Xiaofan , Hu Zeng-Zhen , Huang Bohua 
Published: April 2020

AbstractBased on observational data, this work examines the multi-time-scale feature of the sea surface temperature (SST) variability averaged in the whole North Atlantic Ocean (to be referred to as NASST), as well as its time-scale-dependent connections with El Niño–Southern Oscillation (ENSO) and the North Atlantic Oscillation (NAO). Traditionally, the NASST index is used to characterize the SST trend and multidecadal variability in the North Atlantic. This study found that superimposed on a prominent long-term trend, NASST is nonnegligible at subannual and interannual time scales, compared with that at decadal to multidecadal time scales. Spatially, the interannual variation of NASST is characterized by a horseshoe-like pattern of the SST anomaly (SSTA) in the North Atlantic. It is mainly a lagged response to ENSO through the atmospheric bridge, and NAO plays a secondary role. At the subannual time scale, both ENSO and NAO play a role in generating the fluctuations of NASST and a horseshoe-like pattern in the North Atlantic. Nevertheless, both the ENSO- and NAO-driven variations only explain a small fraction of the variances in both the interannual and subannual time scales. Thus, other factors unrelated to ENSO or NAO may play a more important role. The associated thermodynamical processes are similar at the two time scales; however, the dynamical processes have a significant contribution to the subannual component, but not to the interannual component. Thus, the SSTA averaged in the North Atlantic as a whole varies at different time scales and is associated with different mechanisms.
Simulated Impact of the Tibetan Glacier Expansion on the Eurasian Climate and Glacial Surface Mass Balance during the Last Glacial Maximum
Authors: Liu Yonggang , Wu Yubin , Lin Zhongda , Zhang Yang , Zhu Jiang , Yi Chaolu 
Published: April 2020

Abstract
Glaciers over the Tibetan Plateau and surrounding regions during the Last Glacial Maximum (LGM) were much more extensive than during the preindustrial period (PI). The climate impact of such glacial expansion is studied here using the Community Atmosphere Model, version 4 (CAM4). To cover the range of uncertainty in glacier area during the LGM, the following three values are tested: 0.35 × 106, 0.53 × 106, and 0.70 × 106 km2. The added glacier is distributed approximately equally over the Pamir region and the Himalayas. If 0.70 × 106 km2 is used, the annual mean surface temperature of the glaciated regions would be cooled by ~3.5°C. The annual mean precipitation would be reduced by 0.2 mm day−1 (10%) and 2.5 mm day−1 (24%) over the Pamir region and Himalayas, respectively. The surface mass balance (SMB) of the glaciers changes by 0.55 m yr−1 (280%) and −0.32 m yr−1 (−20%) over the two regions, respectively. The changes in SMB remain large (0.29 and −0.13 m yr−1), even if the area of the Tibetan glacier were 0.35 × 106 km2. Therefore, based on the results of this particular model, the expansion of glaciers can either enhance or slow the glacial growth. Moreover, the expansion of glaciers over the Himalayas reduces summer precipitation in central and northern China by ~0.5 mm day−1 and increases summer precipitation in southern Asia by ~0.6 mm day−1. The expansion of glaciers over the Pamir region has a negligible influence on the precipitation in these monsoonal regions, which is likely due to its large distance from the main monsoonal regions.
Beyond Climate Impacts: Knowledge Gaps and Process-Based Reflection on Preparing a Regional Chapter for the Fourth National Climate Assessment
Authors: Roesch-McNally Gabrielle , Chang Michael , Dalton Meghan , Lowe Scott , Luce Charlie , May Christine , Morishima Gary , Mote Philip , Petersen Alexander “Sascha” , York Emily 
Published: April 2020

AbstractThe Fourth National Climate Assessment (NCA4) provided the most up-to-date understanding of climate change and its effects on the Earth system and on consequences for the United States, including impacts and associated risks, along with approaches to coping with these effects. It is intended to provide guidance to decision-makers in governmental sectors while, in practice, providing guidance for nongovernmental actors. Its regional and topical chapters highlight current knowledge, uncertainties, gaps in knowledge, and emerging threats. The current knowledge and gaps can help set a research agenda to inform future national, regional, and local climate assessments and thereby support better decision-making. The evolution of the assessment, including greater diversity in participation, and more grounded research in the Northwest represents a growing and deepening engagement with more diverse participants. This shift emphasizes the importance of diversity, inclusion, and a greater acknowledgment of multiple ways of knowing, including local and Indigenous knowledge. The Northwest chapter reflects the broader shift in framing from NCA3 to NCA4 to better understand how climate impacts pose risks to things of value in each sector or region. It considers climate impacts through five broad ways in which humans relate to the environment: natural resource economy; heritage and quality of life; water, transportation, and infrastructure; health and social systems; and frontline communities. We reflect on the assessment process and identify three recommendations to improve the assessment outcomes and processes: seek new ways to 1) engage diverse authors and stakeholders and 2) value and integrate epistemic plurality and different knowledge systems, and 3) when gaps are identified, promote research or data collection efforts designed to fill those gaps. Done well, the assessment can build support and knowledge to facilitate community action, leading to broader resilience.
Measuring Lethal Force Performance in the Lab: The Effects of Simulator Realism and Participant Experience
Authors: Blacker Kara J. , Pettijohn Kyle A. , Roush Grant , Biggs Adam T. 
Published: April 2020

Objective The goal of the current study was to compare two types of shooting simulators to determine which is best suited for assessing different aspects of lethal force performance. Background Military and law enforcement personnel are often required to make decisions regarding the use of lethal force. A critical goal of both training and research endeavors surrounding lethal force is to find ways to simulate lethal force encounters to better understand behavior in those scenarios. Method Participants of varying degrees of experience completed both marksmanship and shoot/don’t shoot scenarios on both a video game and a military-grade shooting simulator. Using signal detection theory, we assessed sensitivity as a measure of lethal force performance overall. We used hit rate to assess shooting accuracy and false alarm rate to assess decision making. Results Results demonstrated that performance was correlated across simulators. Results supported the notion that shooting accuracy and decision making are independent components of performance. Individuals with firearms expertise outperformed novices on the military-grade simulator, but only with respect to shooting accuracy, not unintended casualties. Individuals with video game experience outperformed novices in the video game simulator, but again only on shooting accuracy. Conclusion Experience played a crucial role in the assessment of shooting accuracy on a given simulator platform; decision-making performance remained unaffected by experience level or type of simulator. Application We recommend that in expert populations or when assessing shooting accuracy, a military-grade shooting simulator be used. However, with a novice population and/or when interested in decision making in lethal force, a video game simulator is appropriate.
Importance of haemogram parameters for prediction of the time of birth in women diagnosed with threatened preterm labour
Authors: Tolunay Harun Egemen , Elci Erkan 
Published: April 2020

Objective This study aimed to estimate the importance of complete blood count parameters for predicting the timing of birth in threatened preterm labour cases. Methods We performed a retrospective study of 92 patients who were diagnosed with threatened preterm labour (24–34 gestational weeks). The patients were divided into two groups according to the time of birth (group 1: delivered within the first week after diagnosis; group 2: delivered later than 1 week). We compared characteristics and complete blood count parameters between these two groups. Results There were no significant differences in maternal age, body mass index, gravida, parity, haemoglobin levels, and gestational weeks between the two groups. The mean cervical length was 24.24 ± 3.60 mm in group 1 and 30.70 ± 5.32 mm in group 2. There were significant differences in the neutrophil to lymphocyte ratio, white blood cell count, red cell distribution width (RDW), absolute lymphocyte cell count, and absolute neutrophil cell count between the two groups. Conclusion Maternal serum RDW, the neutrophil to lymphocyte ratio, white blood cell count, absolute lymphocyte cell count, and the absolute neutrophil cell count profile could guide clinicians in predicting the time of birth in threatened preterm labour cases.
Ten Modifiable Health Risk Factors and Employees’ Medical Costs—An Update
Authors: Goetzel Ron Z. , Henke Rachel Mosher , Head Michael A. , Benevent Richele , Rhee Kyu 
Published: April 2020

Purpose: To estimate the relationship between employees’ health risks and health-care costs to inform health promotion program design. Design: An observational study of person-level health-care claims and health risk assessment (HRA) data that used regression models to estimate the relationship between 10 modifiable risk factors and subsequent year 1 health-care costs. Setting: United States. Participants: The sample included active, full-time, adult employees continuously enrolled in employer-sponsored health insurance plans contributing to IBM MarketScan Research Databases who completed an HRA. Study criteria were met by 135 219 employees from 11 employers. Measures: Ten modifiable risk factors and individual sociodemographic and health characteristics were included in the models as independent variables. Five settings of health-care costs were outcomes in addition to total expenditures. Analysis: After building the analytic file, we estimated generalized linear models and conducted postestimation bootstrapping. Results: Health-care costs were significantly higher for employees at higher risk for blood glucose, obesity, stress, depression, and physical inactivity (all at P < .0001) than for those at lower risk. Similar cost differentials were found when specific health-care services were examined. Conclusion: Employers may achieve cost savings in the short run by implementing comprehensive health promotion programs that focus on decreasing multiple health risks.
Synchronous detection of vascular tension and nitric oxide release in pulmonary artery: A combined application of confocal wire myograph with confocal laser scanning microscopy
Authors: Zhuang Xiao-Ling , Zhu Zhuang-Li , Zhu Jie-Ling , Lai Su-Mei , Gui Long-Xin , Lin Mo-Jun 
Published: April 2020

Objectives To detect the vascular tension and nitric oxide (NO) release synchronously in mice pulmonary artery, we perform two experiments and present a novel application of confocal wire myograph coupled with the confocal laser scanning microscopy. Methods In the first experiment, viable endothelium-intact mouse pulmonary artery (outer diameter 100–300 μM) rings underwent a one-hour preincubation with a NO-specific fluorescent dye, 4-amino-5-methylamino-2′,7′-difluorofluorescein diacetate Calbiochem (2.5 μM), and then precontracted with phenylephrine (Phen, 10−6 M), and subsequently dilated in acetylcholine (ACh, 10−6 M – 10−4 M). The endothelium-dependent vasorelaxation and NO generation in pulmonary artery rings were simultaneously recorded. In the second experiment, after 30-min incubation with the former NO fluorescent dye, the qualified pulmonary artery rings were co-incubated for another 30 min with a nitric oxide synthase inhibitor, 10−4 M Nω-nitro-L-arginine-methyl-ester (L-NAME), and then pretreated with Phen (10−6 M) followed by ACh (10−5 M). The Ach-induced vasodilation and NO release were recorded simultaneously. Results ACh (10−6 M – 10−4 M) promoted pulmonary artery relaxation and intracellular NO release in a dose-dependent manner. Additionally, L-NAME (10−4 M) significantly attenuated the vasodilatation and the intracellular NO release. Conclusions This combined application visually confirms that the synchronous changes in Ach induced vasodilation and NO release, which provides a new method for cardiovascular research.