bluuebunny/crossref_metadata_2025_split huggingface dataset:
Radiation‐Associated Thyroid Cancer Surveillance and Management in a Cohort of Late Effects Patients
Authors: Koo Eva , Henderson Michael A. , Dwyer Mary , Skandarajah Anita R. 
Published: May 2020

AbstractBackground and aimsCompared to the general population, the incidence of thyroid cancer in childhood and adolescent and young adult malignancy survivors is increased 14.0–18.0 times (CI 11.7–23.8). The cumulative incidence is variably reported as 0.5% by age 45 with 30‐year incidence of 1.3% in women and 0.6% in men. This study aims to evaluate the incidence of radiation‐associated thyroid cancer amongst patients treated with prior radiation to the thyroid followed up in a late effects service. A secondary aim was to assess screening compliance in this cohort.MethodsThe medical records of all patients attending the late effects service from 1 January 2000 to 20 February 2013 were interrogated to identify patients exposed to thyroid irradiation. The screening compliance and incidence of thyroid cancer were assessed for the duration whilst under the guidance of the late effect service. Mode of diagnosis, all imaging and cytology were retrieved from the institutional electronic record. Cytology was categorized according to Bethesda.ResultsFour hundred and sixty‐five patients were exposed to direct or scatter neck irradiation. Compliance with thyroid surveillance was observed in 76.9%. Ultrasound features of microcalcification and increased internal vascularity had a low sensitivity (62.5%) for predicting a malignant nodule, which improved when used in conjunction with a Bethesda IV–VI result (91.7%). However, cytological assessment was not performed in 45.6% of operative cases. Thirty‐three patients had thyroid carcinoma of which 45.4% (n = 15) were incidental. The majority were papillary thyroid cancers (88.9%); of which 12.5% were node positive and 34.4% were multifocal. The incidence of thyroid cancer was elevated 57.6 times compared to the Australian general population (p < 0.001).ConclusionDue to the high incidence of thyroid cancer, this study supports screening in this cohort. However, due to the risk of overtreatment, we endorse further investigation of thyroid nodules with ultrasound‐guided fine‐needle aspiration cytology based on sonographic criteria as for the general population and American Thyroid Association guidelines.
Variations of OH defects and chemical impurities in natural quartz within igneous bodies
Authors: Potrafke Alexander , Breiter Karel , Ludwig Thomas , Neuser Rolf Dieter , Stalder Roland 
Published: May 2020

AbstractIn this study, we present the first systematic dataset on natural variations of OH defect and trace element contents in quartz within igneous bodies. Samples were derived from bore holes of two plutonic bodies from the Krušné Hory/Erzgebirge (German–Czech border), representing typical A-type (Cínovec/Zinnwald granite cupola) and S-type (Podlesí Stock) granite intrusions. Fourier Transform Infrared spectroscopy of quartz was used to investigate the sample set with regard to its OH defect speciation and content. For Zinnwald quartz, IR absorption spectra reveal different lithologies due to changes of the OH defect inventory, enabling a subdivision of the granitic body: (1) hydrothermal greisen quartz of the uppermost part of the intrusion have low OH defect contents (average of 15 µg/g H2O); (2) zinnwaldite granite quartz vary strongly in defect content and show the highest content of the dataset (10–70 µg/g H2O); (3) quartz from an underlying biotite granite have slightly lower, but very uniform contents down to the bottom of the borehole at 1600 m (average 20 µg/g H2O). Infrared spectra of Podlesí quartz reveal a gradual increase in total defect water content with increasing depth over 350 m (30–55 µg/g H2O). Lithium contents in quartz samples from the uppermost part of the Zinnwald intrusion correlate with the occurrence of Li-specific OH defects, while cathodoluminescence (CL) images do not show specific differences. Our findings evidence the potential of OH defects in quartz as a tool to decipher differentiation trends in igneous bodies, and the application of their eroded material for provenance analyses.
Time-based service constraints for inventory systems with commitment lead time
Authors: Ahmadi Taher , Atan Zümbül , de Kok Ton , Adan Ivo 
Published: May 2020

AbstractWe consider a firm which targets a certain level of customer responsiveness with a minimum operational cost. The firm employs a preorder strategy, in which customers place their orders before their actual need based on a predefined time window called commitment lead time and, in turn, they receive a bonus. The firm aims to determine the optimal control policy, which consists of the optimal commitment lead time and the optimal base-stock level, such that the total operational cost is minimized subject to a time-based service level target. We formulate two time-based service criteria to measure the firm’s responsiveness in terms of average customer waiting time and individual customer waiting time in two constraint optimization models called ACO and CCO, respectively. We derive the exact probability distribution of customer waiting time, and we propose exact and heuristic optimization algorithms to solve the ACO and CCO models. We show that the commitment lead time not only increases the firm’s responsiveness level but also decreases the customers’ maximum waiting time. We find that when the firm targets a high level of responsiveness, the value of commitment lead time is more highlighted. We study multiple interesting extensions with two customer classes, random replenishment lead times and a combined model with two time-based service level targets.
Frequent KIT mutations in skin lesions of patients with BRAF wild-type Langerhans cell histiocytosis
Authors: Tóth Béla , Kiss Norbert , Hársing Judit , Kárpáti Sarolta , Csomor Judit , Bödör Csaba , Tímár József , Rásó Erzsébet 
Published: May 2020

AbstractLangerhans cell histiocytosis (LCH) is characterized by mutations of the RAS-RAF-MAPK signaling pathway. We analyzed MAP2K1, NRAS and KIT mutation incidence in skin lesions of BRAF wild-type (wt) LCH patients. We evaluated the occurrence of MAP2K1, NRAS and KIT mutations in seven LCH and one indeterminate cell histiocytosis (ICH) patients. MAP2K1 mutation frequency was found to be 3/7 (42.9%) in LCH and also found in ICH. Similarly, the KIT mutation frequency was found to be equally prevalent (4/7, 57.1%) in LCH and also occurred in ICH. Involvement of KIT exons in LCH-ICH indicated that exon 9/11/18 were equally prevalent followed by exon 13. This exploratory analysis on BRAF-wt LCH revealed a KIT mutation rate comparable to MAP2K1. Although the detected KIT mutations are different from activating mutations found in other KIT-dependent neoplasms, our data suggest that KIT-inhibitors might have a role in treating BRAF-wt LCH patients.
Fermentation process for producing CFAs using Yarrowia lipolytica
Authors: Imatoukene Nabila , Back Alexandre , Nonus Maurice , Thomasset Brigitte , Rossignol Tristan , Nicaud Jean-Marc 
Published: May 2020

Abstract
Past research has sought to improve the production of cyclopropane fatty acids by the oleaginous yeast Yarrowia lipolytica by heterologously expressing the E. coli fatty acid synthase gene and improving cultivation processes. Cyclopropane fatty acids display properties that hold promise for biofuel applications. The E. coli fatty acid synthase gene was introduced into several genetic backgrounds of the yeast Y. lipolytica to optimize lipid synthesis; the mean cyclopropane fatty acid productivity was 43 mg L−1 h−1 on glucose, and the production rate reached its maximum (3.06 g L−1) after 72 h of cultivation in a bioreactor. The best strain (JMY6851) overexpressed simultaneously the E. coli cyclopropane fatty acid synthase gene under a hybrid promoter (hp8d) and Y. lipolytica LRO1 gene. In fed-batch process using crude glycerol as carbon source, JMY6851 strain displayed high lipid accumulation (78% of dry cell weight) and high biomass production (56 g L−1). After 165 h of cultivation, cyclopropane fatty acids represented 22% of the lipids produced; cyclopropane fatty acid productivity (103.3 mg L−1 h−1) was maximal at 72.5 h of cultivation.
Biocontrol ability and volatile organic compounds production as a putative mode of action of yeast strains isolated from organic grapes and rye grains
Authors: Choińska Renata , Piasecka-Jóźwiak Katarzyna , Chabłowska Beata , Dumka Justyna , Łukaszewicz Aneta 
Published: May 2020

AbstractThe inhibiting activity of three yeast strains belonging to Pichia kudriavzevii, Pichia occidentalis, and Meyerozyma quilliermondii/Meyerozyma caribbica genera against common plant pathogens representing Mucor spp., Penicillium chrysogenum, Penicillium expansum, Aspergillus flavus, Fusarium cereals, Fusarium poae, as well as Botrytis cinerea genera was investigated. The yeast strains tested had a positive impact on growth inhibition of all target plant pathogens. The degree of inhibition was more than 50% and varied depending on both the yeast antagonist and the mold. Ethyl esters of medium-chain fatty acids, phenylethyl alcohol, and its acetate ester prevailed among the analyzed volatile organic compounds (VOCs) emitted by yeasts in the presence of the target plant pathogens. Due to the method used, assuming no contact between the antagonist and the pathogen, the antagonistic activity of the yeast strains studied resulted mainly from the production of biologically active VOCs. Moreover, the antagonistic activity was not only restricted to a single plant pathogen but effective towards molds of different genera, making the yeast strains studied very useful for potential application in biological control.
FOXO transcription factor family in cancer and metastasis
Authors: Jiramongkol Yannasittha , Lam Eric W.-F. 
Published: May 2020

AbstractForkhead box O (FOXO) transcription factors regulate diverse biological processes, affecting development, metabolism, stem cell maintenance and longevity. They have also been increasingly recognised as tumour suppressors through their ability to regulate genes essential for cell proliferation, cell death, senescence, angiogenesis, cell migration and metastasis. Mechanistically, FOXO proteins serve as key connection points to allow diverse proliferative, nutrient and stress signals to converge and integrate with distinct gene networks to control cell fate, metabolism and cancer development. In consequence, deregulation of FOXO expression and function can promote genetic disorders, metabolic diseases, deregulated ageing and cancer. Metastasis is the process by which cancer cells spread from the primary tumour often via the bloodstream or the lymphatic system and is the major cause of cancer death. The regulation and deregulation of FOXO transcription factors occur predominantly at the post-transcriptional and post-translational levels mediated by regulatory non-coding RNAs, their interactions with other protein partners and co-factors and a combination of post-translational modifications (PTMs), including phosphorylation, acetylation, methylation and ubiquitination. This review discusses the role and regulation of FOXO proteins in tumour initiation and progression, with a particular emphasis on cancer metastasis. An understanding of how signalling networks integrate with the FOXO transcription factors to modulate their developmental, metabolic and tumour-suppressive functions in normal tissues and in cancer will offer a new perspective on tumorigenesis and metastasis, and open up therapeutic opportunities for malignant diseases.
Kosakoniacowanii as the New Bacterial Pathogen Affecting Soybean (Glycine max Willd.)
Authors: Krawczyk Krzysztof , Borodynko-Filas Natasza 
Published: May 2020

AbstractSoybean (Glycine max Willd.) is one of the most economically important crops in the world, and it’s importance continuously increasing. Routine screening of Polish soybean fields has revealed an outburst of a new disease of soybean plants, with symptoms resembling those described for the bacterial blight, caused by Pseudomonas syringae pv. glycinae, and bacterial pustule caused by Xanthomonas axonopodis pv. glycines. The symptoms were observed in the field, on the leaves of investigated plants. The goal of this paper was the identification and characterization of the causal agent of that disease. Our studies have excluded virus and fungi and pointed the bacteria as the disease causal agent. Identification based on biological (Biolog Gen III), and molecular methods (16S rDNA, and gyrB sequencing, PCR species-specific testing) revealed that the soy pathogenic bacteria is Kosakonia cowanii species. We believe that the presence of such pathogens in the environment pose a serious threat for the crops and is also a warning, that in the near future we may be faced a new type of bacterial pathogens: both epi- and endophytic, abundant in the natural environment, with huge metabolic potential, and ability of quick colonization of new ecological niches and hosts, e.g. opportunistic pathogens of plants or humans. What’s important is that the plant-pathogenic and plant-epiphytic “environmental” strains of such species can be distinguished from each other only post factum, in the pathogenicity tests. They are indistinguishable or very hard to distinguish morphologically, biochemically or molecularly.
Advances in exploring activity cliffs
Authors: Stumpfe Dagmar , Hu Huabin , Bajorath Jürgen 
Published: May 2020

AbstractThe activity cliff (AC) concept is of comparable relevance for medicinal chemistry and chemoinformatics. An AC is defined as a pair of structurally similar compounds with a large potency difference against a given target. In medicinal chemistry, ACs are of interest because they reveal small chemical changes with large potency effects, a concept referred to as structure–activity relationship (SAR) discontinuity. Computationally, ACs can be systematically identified, going far beyond individual compound series considered during lead optimization. Large-scale analysis of ACs has revealed characteristic features across many different compound activity classes. The way in which the molecular similarity and potency difference criteria have been addressed for defining ACs distinguishes between different generations of ACs and mirrors the evolution of the AC concept. We discuss different stages of this evolutionary path and highlight recent advances in AC research.
Efficiency and Mechanism of Palladium(II) Extraction from Chloride Media with N-Hexadecylpiperidinium Chloride
Authors: Cieszynska Anna , Wieczorek Daria 
Published: May 2020

AbstractN-hexadecylpiperidinium chloride ([PIP16]Cl) has been synthesized and examined as a reagent for extractive recovery of palladium(II) from hydrochloric acid solutions. The effect of the contact time between the two phases, temperature and the concentration of palladium(II) ions, the extractant and HCl, were also evaluated. The anion-exchange mechanism of palladium(II) extraction with [PIP16]Cl was confirmed by Job’s method, UV–VIS, IR and1H NMR analysis. The extraction of palladium(II) from 0.1 mol·L−1HCl solution with [PIP16]Cl in toluene was very effective and amounted to almost 100%. Increases in both HCl and NaCl concentrations and increasing temperature had negative effects on the effectiveness of palladium(II) extraction. [PIP16]Cl also exhibited selectivity to extraction of palladium(II) over some other metals: rhodium(III), iron(III), aluminium(III), copper(II) and lead(II). From among the examined stripping solutions the most effective were 0.5 mol·L−1aqueous solution of ammonia and 0.1 mol·L−1thiourea in 0.1 or 1 mol·L−1HCl. The percentage of palladium(II) stripped from loaded organic phase reached nearly 100%. The feasibility of regeneration of [PIP16]Cl and its reuse in subsequent extractions was also investigated.
Interaction of gold nanoparticles with cholesterol oxidase enzyme in bionanocomplex—determination of the protein structure by Fourier transform infrared spectroscopy
Authors: Wojnarowska-Nowak Renata , Polit Jacek , Sheregii Eugen M. 
Published: May 2020

AbstractThe bionanocomplexes of proteins and nanoparticles are promising bionanomaterials for medical purposes like the sensitive and the selective diagnostic devices, as well as, the modern therapeutic agents. The presence of the noble metals allows for obtaining the plasmonic effect and for generating the surface-enhanced Raman scattering phenomenon (SERS). The knowledge of the nanoparticle interaction with the protein molecule is extremely necessary for the development of the suitable product. The interaction of the bionanocomplex components can cause the conformational changes in the protein structure and the modification of surface properties of the nanoparticles. In the case of the binding of the gold nanoparticles with the enzymes, it is critical to preserve the catalytic activity, which is connected with maintaining the proper molecule structure. In the presented study, the Fourier transform infrared spectroscopy was used for the investigation of the interaction and the structural properties of the cholesterol oxidase (ChOX)—the gold nanoparticles bionanocomplex. The ChOX is one of the most important enzymes for the medical diagnostics and the promising antibacterial agent. The infrared spectra of the AuNP-ChOX bionanocomplex and the ChOX in different forms (the solid and the suspended in the buffer) are presented and discussed. The obtained spectra include the considerable number of the absorption lines identified and attributed to the corresponding chemical bonds and the functional groups of the protein. The analysis of the obtained infrared spectra revealed the important information about the changes of the protein structure after the immobilization on the gold nanoparticles. Moreover, it allows to recognize the differences in the structure of the protein in a different environment, which is of great importance in the context of bionanotechnology.
Gene expression variability between randomly and targeted transgene integration events in tobacco suspension cell lines
Authors: Kirchhoff Janina , Schiermeyer Andreas , Schneider Katja , Fischer Rainer , Ainley W. Michael , Webb Steven R. , Schinkel Helga , Schillberg Stefan 
Published: May 2020

AbstractGenome editing tools such as zinc-finger nucleases provide novel strategies for genetic manipulation in plants. Unlike agrobacterium-mediated or direct gene transfer, which introduce genes randomly into the genome and thereby potentially resulting in high variation of gene expression, the targeted gene addition provides predictable integration of DNA sequences into a specified location of the plant genome. We investigated whether various independent cell lines that all contain a transgene placed in the same genomic locus by zinc-finger nuclease-mediated homologous recombination (HR) would yield a more reproducible and homogeneous level of expression compared to integration events generated via agrobacterium-mediated transformation at random sites. The variance of gene expression of targeted HR events and random integration events was analyzed inNicotiana tabacumL cv. Bright Yellow 2 (BY-2) suspension cells by measuring protein amount produced from the transgene by flow cytometry, thus providing the first report on positional effects of marker gene expression in a quickly proliferating plant suspension cell line. Marker protein levels of targeted HR and single-copy random events covered a similar range; however, the uniformity of protein expression in a given cell line was significantly higher in targeted events than in lines with randomly inserted transgene; the same is true for the overall viability of protoplasts from HR lines. In conclusion, using targeted insertion into a qualified locus of a well-characterized line leads to more reliable results than random insertion into the genome.
Preparation and characterization of activated carbons obtained from the waste materials impregnated with phosphoric acid(V)
Authors: Zięzio Magdalena , Charmas Barbara , Jedynak Katarzyna , Hawryluk Monika , Kucio Karolina 
Published: May 2020

AbstractIn the paper spent coffee grounds were used as a precursor to obtain activated carbons. The raw material was impregnated with phosphoric acid(V) at the different impregnations ratios: 0.5, 1, 1.5, 2. Carbonization was carried out according to two procedures differing in activation atmosphere (N2 or CO2). The obtained activated carbons were characterized on the basis of low-temperature nitrogen adsorption/desorption, thermal analysis, potentiometric titration method, X-ray diffraction, Raman spectroscopy and scanning electron microscopy. Carbons obtained according to procedure 2 (activation in CO2) were characterized by better developed porosity, e.g. surface (SBET to 720.9 m2/g) and pore volume (Vp to 0.334 cm3/g). All obtained carbons had surface acidic (mainly carboxyl) groups and exhibited the amorphous structure. The thermal analysis showed that the obtained materials were thermally stable up to the temperature ~ 420 °C.
When Did the Health Gradient Emerge? Social Class and Adult Mortality in Southern Sweden, 1813–2015
Authors: Bengtsson Tommy , Dribe Martin , Helgertz Jonas 
Published: May 2020

AbstractAcross today’s developed world, there is a clear mortality gradient by socioeconomic status for all ages. It is often taken for granted that this gradient was as strong—or even stronger—in the past when social transfers were rudimentary and health care systems were less developed. Some studies based on cross-sectional data have supported this view, but others based on longitudinal data found that this was not the case. If there was no gradient in the past, when did it emerge? To answer this question, we examine social class differences in adult mortality for men and women in southern Sweden over a 200-year period, using unique individual-level register data. We find a systematic class gradient in adult mortality emerging at ages 30–59 only after 1950 for women and after 1970 for men, and in subsequent periods also observable for ages 60–89. Given that the mortality gradient emerged when Sweden transitioned into a modern welfare state with substantial social transfers and a universal health care system, this finding points to lifestyle and psychosocial factors as likely determinants.