bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cartographie des voies sous-marines en Asie du Sud-Est
Authors: Bonnet François-Xavier 
Published: March 2020

La recherche océanographique militaire, dont les études bathymétriques, est devenue un enjeu géopolitique majeur en Asie du Sud-Est. Dans un double contexte de rivalités de pouvoir entre la Chine et les États-Unis et de rivalités territoriales entre quatre pays de l’ASEAN et la Chine en mer de Chine méridionale, certains pays de l’Asie du Sud-Est se sont dotés ou vont se doter d’une sous-marinade et de matériel de lutte anti-sous-marine. L’exemple des Spratleys montre la grande sensibilité de la question de la bathymétrie. Les îles Spratleys ou Dangerous Ground , autrefois une zone dangereuse à éviter, sont devenues une région stratégique à haut risque : un sous-marin nucléaire lanceur d’engins situé dans les hauts-fonds du Dangerous Ground pourrait menacer une grande partie de la population de la planète.
Une clairvoyance suspecte
Authors: Chabert Catherine 
Published: March 2020

L’auteure propose une étude des interactions du refoulement chez le patient et l’analyste dans une double perspective, clinique et métapsychologique. La douleur psychique nécessite un refoulement récurrent, protégé par le transfert narcissique et idéalisant. La contrainte de la clairvoyance s’impose dans l’identification de l’analyste au désir de son patient : au-delà de la séduction, l’attente transférentielle active chez l’analyste une identification exaltante à la bonne personne susceptible de faire surgir et révéler les formations enfouies dans l’inconscient. Le transfert latéral permet alors d’ouvrir une voie de passage vers les fantasmes de désir et la confrontation à leurs empêchements dans la cure.
Confiance et contexte : une approche non cognitiviste des normes
Authors: Hunyadi Mark 
Published: March 2020

Le contextualisme moral passe pour un culturalisme relativiste, notamment aux yeux de la théorie cognitiviste des normes de Habermas, ancrée dans une théorie de la communication langagière. Pour éviter ce cognitivisme discursif qui indexe les normes universelles à la vérité, il faut ancrer les normes en deçà de la communication langagière et de la logique des prétentions à la validité qui y est inscrite : dans notre rapport pratique au monde, caractérisé d’abord par la confiance qu’on met en lui (la confiance étant définie comme un pari sur des comportements attendus). L’article montre que les normes (qui fixent les attentes de comportement réciproques des acteurs) renvoient elles aussi à cette relation fiduciaire élémentaire, les détachant de leur lien cognitiviste à la vérité.
« Godefroy et le mouvement des peuples ». Singulier et collectif dans l’écriture de la guerre (XII e –XIII e siècles)
Authors: Milland-Bove Bénédicte 
Published: March 2020

Le modèle médiéval, épique ou romanesque, sert souvent de repoussoir aux écrivains modernes qui prétendent donner une vision moins idéalisée des conflits, en montrant les foules dans leur quotidien parfois terre-à-terre plutôt que l’individu héroïque. Dans sa Poétique du récit de guerre , Jean Kaempfer montre comment le récit de guerre moderne se construit en contraste avec l’ancien récit de bataille, mais le modèle qu’il envisage est avant tout le modèle antique du récit « à la César ». En faisant dialoguer images, récits et procédés de textes antiques, médiévaux et modernes voire contemporains, le présent article voudrait proposer des pistes pour intégrer les récits médiévaux des xii e - xiii e siècles à une poétique générale de la guerre. Celle-ci sera envisagée à travers une série de tensions (entre singulier et collectif, ordre et désordre, dicible et indicible, topique et invention) présentes à chaque époque plutôt que comme un mouvement vectorisé d’un type d’écriture à un autre. On fera ainsi l’hypothèse d’une écriture « continuée » ou « collective » de la guerre, avec une circulation intertextuelle et mémorielle des discours et motifs, entraînant une sorte de « guerre des récits », mais se nourrissant aussi de realia sans cesse réactivées.
L’artisanat horloger au xixe siècle
Authors: Starck Perrine 
Published: March 2020

L’histoire de l’horlogerie s’étale sur plusieurs siècles, et a traversé les époques en s’améliorant continuellement. Au xixe siècle, alors que la discipline s’est élevée au rang d’art, des amateurs s’y intéressent et s’approprient les savoir-faire de l’artisanat horloger. Dans des musées comme le musée des Arts et Métiers, riche d’une collection conséquente d’instruments horaires de tous âges, prennent alors leur place des pendules dont l’originalité se retrouve tant dans leur fabrication, que dans leur affichage des heures et leurs complications.
Reseña de The Routledge Handbook of Spanish Language Teaching de Muñoz-Basols, Gironzetti y Lacorte (eds.)
Authors: Duque Eladio 
Published: March 2020

Reseña de The Routledge Handbook of Spanish Language Teaching. Metodologías, contextos y recursos para la enseñanza del español L2 de Muñoz-Basols, Gironzetti y Lacorte (eds.). Londres: Routledge, 2019. ISBN 9781138182905.
Medidas de temperatura em ambiente interno usando a Plataforma Arduino
Authors: Ritter Greice Scherer , Cavalheiro Eliezer Oliveira , Silva Ronaldo Barcelos e , Schmidt Leonardo Da Rosa , Maldaner Silvana 
Published: March 2020

The paper presents the results of a study with temperature measurements using low cost sensors connected to an Arduino microcontroller. To perform the study, three sensors widely used for monitoring environmental conditions with Arduino. The selected sensors were the LM35DZ (analog sensor) and DHT11 and DHT22 (digital sensors). The LM35DZ sensor is a sensor known to be an analog sensor that has linear temperature response with voltage. The DHT11 sensor measures temperature and humidity simultaneously. To measure temperature the DHT11 sensor uses a temperature-sensitive resistor and has a measurement range from 0 to 50 °C, with an uncertainty ± 2% ° C. The DHT22 has a measurement range -40 to 80 ° C and an uncertainty ± 1% ° C. Simultaneous temperature measurements with the three sensors showed good performance in indoor situations, showing the maximum and minimum temperatures of a daily temperature cycle.
Linfoma não-Hodgkin, manifestação no sistema estomatognático – Relato de caso clínico
Authors: Gomes Rita Fabiane Teixeira , Piazza José Luiz , Castelo Edilson Fernando 
Published: March 2020

Linfomas são transformações neoplásicas de células linfoides, o Linfoma Não-Hodgkin raramente ocorre na cavidade oral, os casos intraorais podem manifestar-se como inchaço, dor, dormência, mobilidade dentária ou linfadenopatia cervical, os achados radiográficos podem assemelhar-se a osteomielites, neoplasias secundárias ou lesões odontogênicas. Pode ocorrer aumento de volume em tecido mole e não cicatrização após extrações dentárias - sinais suspeitos de malignidade. Os linfomas são lesões malignas agressivas, com alto potencial de cura através de quimioterapia associada ou não à radioterapia, se diagnosticados precocemente. A cirurgia geralmente não é indicada. O prognóstico é bom para doenças localizadas, sendo menos favorável para doença disseminada. Cabe ao cirurgião-dentista diagnosticar manifestações estomatognáticas, contribuindo para o tratamento médico efetivo. O objetivo deste estudo é o relato de caso clínico de paciente com lesão osteolítica na mandíbula, com diagnóstico de Linfoma não-Hodgkin difuso de grandes células B a partir da investigação clínica multidisciplinar.
Descarte de medicamentos: avaliação do conhecimento dos produtores da atividade leiteira
Authors: Batista Nadine Neto , Mendes Graziella Alebrant , Carvalho Themis Goretti Moreira Leal de , Negrete Bruna Barcellos , Bulegon Jovana Simonetti , Schäfer Marcos Guilherme , Martins Valeska , Deuschle Viviane Cecília Kessler Nunes 
Published: March 2020

O objetivo desse estudo foi avaliar a prática do uso e do descarte de medicamentos entre produtores da rede leiteira da região noroeste do Estado do Rio Grande do Sul. O estudo foi conduzido mediante a aplicação de um questionário e a amostra foi constituída por 46 participantes. Os resultados demonstram que a maioria dos participantes apresenta medicamentos em suas residências (98%). Destes, 66% afirmam usar medicamentos por conta própria, 22% reutiliza medicamentos de tratamentos anteriores, 65% realiza o descarte em lixo comum e 9% descarta em vaso sanitário. Em relação à “farmácia caseira” o medicamento mais armazenado foi o paracetamol (78%). A maioria dos participantes (74%) relataram não ter recebido nenhuma informação a respeito do assunto. Neste sentido, observa-se que a maioria da população descarta os medicamentos de forma inadequada e a falta de orientações é o principal fator que leva a esta prática.
Genotyping of the site of the mtDNA of representatives of the Cetoniinae subfamily
Authors: Balashenko N. A. , Semenyak A. A. , Kornilkova A. S. , Sen'kevich I. A. , Prishchepchik O. V. , Dromashko S. E. 
Published: March 2020

Aim. Description of the results of molecular genetic research of insects-representatives of the subfamily Cetoniinae (flower chafer). Methods. For the analysis, methods of low-traumatic sampling of material for DNA isolation, polymerase chain reaction method, sequencing of the region of mtDNA COI gene were used. Results. A technique for sampling biological material for isolating DNA from the hind legs of individuals was proposed, which allowed not to remove from the habitat the individuals of the most valuable species, collected on the territory of the Republic of Belarus. A collection of DNA samples of representatives of the subfamily Cetoniinae was prepared. The haplotypes Oxythyrea funesta (Poda, 1761) and Protaetia aeruginosa (Linnaeus, 1767) of representatives of the populations inhabiting the territory of Belarus have been identified. Conclusions. There were significant differences in haplotypes among representatives of the Belarusian and European parts of their areal.
Keywords: molecular markers, mtDNA, DNA-barcoding, insects, subfamily Cetoniinae.
Genome specificity of primers to puroindoline genes
Authors: Sozinova O. I. , Kozub N. A. , Sozinov I. A. , Blume Ya. B. 
Published: March 2020

Aim. Genome specificity of primers for amplification of complete coding sequences of puroindoline genes was studied. Methods. PCR with gene-specific primers was performed for amplification of puroindoline genes of wheat and related species. Results. The primer pair designated PinaDH is not gene-specific as it yields two products of amplification of the genes Pina-1 and Gsp-1. This primers pair is not specific for the Pina-1 gene of the genome V of D. villosum. The primer pairs designated PinaCM and Pinb are gene-specific as they produce one amplification product of respective length. We have demonstrated that the PinaCM primer pair is also specific for the genomes D, E and V, and the Pinb primer pair is not specific for the genome V. Conclusions. Gene and genomic specificity of primers for puroindoline genes was refined. Primers suitable for further direct sequencing of coding parts of puroindoline genes of wheat and related species were chosen.
Keywords: puroindoline, Triticum aestivum, Aegilops, Dasypyrum, genome specificity.
Agrobacterium-mediated in planta genetic transformation of winter wheat (Triticum aestivum L.)
Authors: Mykhalska S. I. , Komisarenko A. G. , Kurchii V. M. , Tishchenko O. M. 
Published: March 2020

Aim. To optimize the agrobacterium-mediated method of winter wheat transformation (Triticum aestivum L.); to select the conditions and period of inoculation to effectively transfer the genes during pollination. Methods. Agrobacterium-mediated in planta genetic transformation of winter wheat (Triticum aestivum L.) during pollination. Results. The conditions for agrobacterium-mediated transformation method of winter wheat during natural (frequency pollination was 1 %) and non-natural (frequency pollination was 4 %) pollination were defined. Conclusions. The possibility of integrating transgenes into the genome of winter wheat plants by the method of Agrobacterium-mediated transformation in planta in the process of forced and natural pollination is demonstrated. It is found that the transformation efficiency to a large extent depends on the plant genotype and the method of carrying out the transformation procedure. The selection of transgenic plants under water deficit conditions allowed to identify the plants with functional transgene. The signs of functioning transgene have been remaining in the next generation of genetically modified winter wheat.
Keywords: Triticum aestivum L., Agrobacterium-mediated transformation in planta, transgenic plants, seeds.
Presowing radiation exposure of Chamomilla recutita L. seeds affects on flavonoid accumulation
Authors: Pchelovska S. A. , Litvinov S. V. , Shylina Yu. V. , Lystvan K. V. , Zhuk V. V. , Sokolova D. O. , Tonkal L. V. , Salivon A. G. , Nesterenko O. G. 
Published: March 2020

Aim. Presowing radiation exposure of seeds is offered to apply as modification factor of secondary metabolites accumulation in medicinal plants. Aim of studying was to choose doses of both gamma- and X-ray exposure of Chamomilla recutita L. seeds that would lead to increasing synthesis of secondary metabolites and biomass. Methods. Both X- and gamma-radiation exposure of seeds, extraction of flavonoids, spectrophotometry assessment of flavonoid extracts, qualitative and semi-quantitative high effective liquid chromatography. Results. Presowing radiation exposure of Chamomilla recutita L. seeds increased both flavonoid production over control and yield of pharmacologically valuable raw material – inflorescence number per square. Chromatographical analyses indicated stable qualitative composition of Chamomilla recutita L. ethanol extracts; the exposure did not lead to synthesis of de novo components. Conclusions. Obtained data validate approach to apply presowing radiation exposure of Chamomilla recutita L. seeds (5–50 Gy dose rate) as modification factor increasing both its productivity and pharmaceutical value of medicinal plants.
Keywords: Chamomilla recutita L., stimulating productivity, flavonoids, presowing exposure.
Noncanonical DNA-binding properties of potential components of an antitumor composition (cytokine AIMP1/p43, lectin SNA, izatizon), which are able to affect different targets
Authors: Tsuvariev O. Yu. , Starozhuk O. V. , Karpova I. S. , Palchykovska L. G. , Zayika L. A. , Shyrina T. V. , Lylo V. V. , Kornelyuk O. I. 
Published: March 2020

Aim. To search the DNA-binding properties of various drugs promising for the creation of the antitumor composition (AIMP1/p43, SNA-I, izatizon), as well as their ability to influence on key matrix processes – DNA transcription and replication, which are a sensitive target for the action of many pharmacological drugs. Methods. The recombinant protein AIMP1/p43 was obtained from supernatant of E.coli lysed cells by metallic chelating chromatography on a Ni‑NTA‑agarose column. Also there were used the elderberry bark lectin SNA-I (LECTINOTEST, Ukraine), and the preparation of own production izatizon (IMBG NAS of Ukraine). The DNA-binding ability of the drugs was investigated by EMSA. As a transcription test system, an enzyme complex of DNA-dependent RNA polymerase of T7 bacteriophage and plasmid DNA pTZ19R * were used. PCR with Taq DNA polymerase and the plasmid pTZ19R * was used as the model replicative system. Results. Experimental conditions, in which the specificity of each drug action appeared, were found: the cytokine AIMP1/p43 and the SNA-I demonstrated the ability to bind to DNA by the EMSA test; izatizon induced structural changes in DNA, blocked the amplification of DNA during the PCR process, and also in vitro transcription with the participation of DNA-dependent RNA polymerase of the bacteriophage T7. Conclusions. Each of the selected drugs, which according to the literature data have antitumor potential, has demonstrated the ability to interact with the model supercoiled plasmid DNA pTZ19R *. The different ways of the selected drugs influence on DNA allows us to expect their effective combined action and synergism in case of their application as a common antitumor composition.
Keywords: cytokine AIMP1/p43, SNA-I, izatizon, EMSA method, transcription inhibiting, amplification blocking.
Transcriptional factor Cat8 is involved in regulation of xylose fermentation in engineered Saccharomyces cerevisiae
Authors: Dzanaieva L. S. , Dmytruk K. V. , Sybirny A. A. 
Published: March 2020

Aim. The aim of this work is the construction of cat8Δ strain on the base of xylose-fermenting S. cerevisiae strain and evaluation of the xylose fermentation rate. Methods. The CAT8 deletion cassette harboring natNT2 gene flanking with 5’ and 3’ non-coding regions of CAT8 gene has been constructed. After transformation by the cassette the cat8Δ strain was selected on the nourseothricin containing medium. Xylose fermentation experiments of constructed strain was performed in mineral medium supplemented with xylose under oxygen-limited conditions. Results. Xylose-fermenting cat8Δ S. cerevisiae strain has been constructed by homologous recombination of the CAT8 deletion cassette with target sequences in the genome of GS010 strain. The cat8Δ strain possessed increase in ethanol accumulation, ethanol yield, rate of ethanol production and productivity of ethanol synthesis relative to the parental GS010 strain for 9.5, 6, 20 and 12 %, respectively. Conclusions. The mutant of the xylose-fermenting S. cerevisiae strain with knock out of the CAT8 gene coding for transcriptional activator, has been constructed. The cat8Δ mutant showed 9.5 % increase in ethanol production from xylose relative to parental strain.
Keywords: alcoholic fermentation, xylose, S. cerevisiae, Cat8.
The analisis of human MGMT gene orthologous in protests
Authors: Pidpala O. V. , Lukash L. L. 
Published: March 2020

Aim. The intron sequences of orthologous О6-methylguanin-DNA methyltransferase (MGMT) genes in Protists on the early stages of their formation in eukaryotic organisms have been analysed. Methods. Homologous regions have been defined by the program BLASTN 2.6.1. Nucleotide sequences of the bacterial and mitochondrial group II introns have been taken from Database for Bacterial Group II Introns. Searching and identifying the MGEs have been realized by using CENSOR. Results. It has been shown that the evolution of the gene does not always coincide with the evolution of the organism. This is shown on the example of intron loss and gain in social amoebae Dictyostelium. Also it has been found the fragmentary nature of homology between various introns and exons of the orthologous genes. Conclusions. The obtained results allow offer a suggestion about the endogenous mosaic character of the evolutional formation of the gene structural units. Keywords: О6-methylguanin-DNA methyltransferase (MGMT) gene orthologous, Protists, gene evolution, spliceosomal introns, intron loss and gain.
Analysis of Glu-1 loci in samples of rare wheat species and their hybrides
Authors: Sichkar S. M. , Velykozhon L. H. , Dubrovna O. V. , Morgun B. V. 
Published: March 2020

Aim. Determination of the allelic composition of Glu-1 loci in samples of rare wheat species and their hybrids with spring bread wheat. Methods. PCR analysis. Results. In existing collection samples of the rare wheat species the alleles of the a/c loci Glu-A1 were found, while allele b was found only in T.dicoccum, var. volgense (Emmer Kokchetavskaya). An additional amplicon with a length of 450 bp was found in the Glu-A1 locus in the sample (T. dicoccum × Dasypyrum villosum), and in (Ae. ventricosa × T. dicoccum) another one a length of 700 bp in genome B, was found require further research. Among hexaploid wheat, it was revealed genotypes differing in the presence of alleles a and d of the locus Glu-D1. The hybridity of the received forms has been confirmed, as evidenced by the identification of both parent components in the hybrids or alleles of the Glu-D1 locus of bread wheat in hybrids with emmer. Conclusions. Analysis of the allele composition of Glu1 locuses in samples of rare wheat species and their hybrids with bread wheat allowed to select genotypes and hybrid combinations that may be promising for further breeding work.
Keywords: Triticum spelta L., T. dicoccum, hybrids, PCR analysis, Glu-1 locus.
Description of genetic polymorphism and cytological stability of Avena sativa L., Avena sterilis L. and their interspecies hybrids
Authors: Sycheva Y. A. , Drobot N. I. , Bondarevich Y. B. , Solovey L. A. , Khaletsky S. P. , Dubovets N. I. 
Published: March 2020

Aim. A study of cytological stability and genetic polymorphism of SSR loci in Avena sativa L., Avena sterilis L. and their interspecies hybrids. Methods. Interspecific hybridization in combination with cytological and molecular-genetic analysis. Results. The genetic polymorphism of six SSR loci of 15 Avena sativa L. varieties, 12 Avena sterilis L. lines and their interspecies hybrids was studied. Highly informative (PIC from 0.57 to 0.89) SSR markers that can be used to differentiate genetically related genotypes of oats are revealed. The results of cytological analysis showed a high level of bivalent pairing at meiotic metaphase I (97.0 to 99.86 %) in A. sativa × A. sterilis hybrids, as well as the presence of a significant number of cells with bridges at the anaphase I stage of meiosis. Conclusions. The high level of bivalent chromosome pairing and the identification of forms with a high meiotic index indicate the possibility of creating cytologically stable hybrids of A. sativa × A. sterilis. The presence of a significant number of cells with bridges at the anaphase I stage may serve as an indirect confirmation of chromatin exchange between the parental species, which creates the prerequisites for the inclusion in the genome of oats alien genetic material in the form of translocations.
Keywords: Avena sativa L., Avena sterilis L., cross-species hybridization, cytological stability, SSR-markers.
The variability of the trait thickness of the straw second internode (TS-2M) of soft winter wheat (Triticum aestivum L.) of different origin
Authors: Tishchenko V. M. , Dinets O. M. 
Published: March 2020

Aim. Detailed examination of different studies showed that the thickness of the straw second internode of soft winter wheat is not sufficient investigated. Methods. The article presents the results of an experiment for the study of the formation and the variability of the trait thickness of the straw second internode (TS-2M) in different soft winter wheat (Triticum aestivum L.) varieties and breeding lines. There are forty one wheat varieties of different origin presented in this study. Results. It was established that the trait TS-2M significantly correlated with the main yield traits. Therefore, the selection of productive genotypes in the selection process based on TS-2M is effective. Conclusions. The statistical analysis of winter wheat varieties based on the trait TS-2M showed that the level of formation and variability of this trait is genetically determined.
Keywords: winter wheat, the second internode (ТS-2М), genetic coefficient of variation (CV%).