bluuebunny/crossref_metadata_2025_split huggingface dataset:
Brain electrical activity to tones of increasing intensity in fibromyalgia: effects of attentional mechanisms and medication.
Authors: Samartin-Veiga Noelia , González-Villar Alberto Jacobo , Triñanes Yolanda , Gómez-Perretta Claudio , Carrillo-de-la-Peña María Teresa 
Published: April 2020

Abstract
Background

Fibromyalgia (FM) has been associated to an increased central response to noxious somatosensory stimulation, which may be generalized to other sensory modalities. However, the evidence supporting generalized hypervigilance or increased sensory processing is not conclusive. To clarify this, we studied the brain electrical activity associated to auditory stimuli varying in intensity in patients with FM, considering the effects of attentional mechanisms and medication.
Methods

We performed two experiments using different sequences of stimuli (engaging bottom-up vs. top-down attention). In Study 1, we recorded auditory event-related potentials (AEPs) from 50 patients with FM and 60 healthy controls (HCs), using 72 stimuli of each intensity level (70, 80, 90, 105dB) presented randomly within the sequence. In Study 2, the AEPs were recorded from 28 patients with FM and 30 HCs using 3 intensity levels (70, 90, 105dB) of stimuli presented in blocks. For each experiment, we analyzed intensity and group effects on N1-P2 peak-to-peak amplitude using mixed-model ANOVAs and, only for the FM group, the effect of Group of Medication (with vs. without effect on the CNS) and the correlation (Pearson’s r) between AEPs and clinical variables.
Results

In general, the N1-P2 amplitudes were larger at Cz than Fz and, increased as the tone intensity increased. Contrary to the expectation, the patients showed a trend of reduced AEPs to the loudest tones in study 1 and no significant differences with the HCs in study 2. Medication with central effects significantly reduced AEP amplitudes, while no significant relationships between the N1-P2 amplitude/intensity function and patient symptoms were observed.
Conclusions

Using a large sample, the findings do not provide evidence of augmented central processing of auditory stimuli in FM, nor of its relationship with the symptoms severity. Nevertheless, given the observed effect of medication, we could not discard the role of sensory amplification as an underlying pathophysiological mechanism in fibromyalgia.
Loss of HOXB3 promotes development of hormone receptor negative breast cancer
Authors: Zhu Lizhe , Yu Shibo , Jiang Siyuan , Ge Guanqun , Yan Yu , Zhou Yuhui , Niu Ligang , He Jianjun , Ren Yu , Wang Bin 
Published: April 2020

Abstract
BackgroundThe homobox (HOX) gene family as a transcription factor encoding a specific nuclear protein is essential for embryonic development, differentiation, and homeostasis. The role of HOXB3 protein varies in different tumors. This study aims to explore the role of the HOXB3 gene in breast cancer.MethodDifferentiated expressed genes were screened by analyzing metastatic breast cancer gene chip data in TCGA and GEO database. The function of selected HOXB3 gene was also analyzed by GEPIA, Kaplan-Meier Plotter, Breast Cancer Gene-Expression Miner and metascape. Molecular biology methods such as qRT-PCR, western blot and IF was used to verify bio-informatics findings.ResultsBoth bio-informatics analyses and western blot showed that HOXB3 was lost in breast cancer compared to normal breast tissue. Survival analysis also showed that lower expression of HOXB3 was associated with poor prognosis. Bio-informatics analyses further showed that HOXB3 was positively correlated with hormone receptors. qRT-PCR, immunofluorescence and western blot also confirmed that HOXB3 had the highest expression in the immortalized breast epithelial cell line MCF-10A, lower in luminal breast cancer cell line T47D and the lowest in triple negative breast cancer (TNBC) cell line MDA-MB-231. Metascape for GO analysis of GEO data provided possible mechanism that HOXB3 could positively regulate cell adhesion, inhibit cell proliferation and activate immune response in breast cancer, and considered that HOXB3 might cause cell malignant transformation through the above pathways.ConclusionIn summary, HOXB3 expression was decreased in breast cancer, especially in hormone receptor-negative breast cancer. The lower expression of HOXB3 was associated with poor prognosis. It might become a new biomarker to predict prognosis of breast cancer.
Finite element analysis of the initial stability of arthroscopic ankle arthrodesis with three-screw fixation: posteromedial versus posterolateral home-run screw
Authors: Wang Sen , Yu Jian , Ma Xin , Zhao Dahang , Geng Xiang , Huang Jiazhang , Wang Xu 
Published: April 2020

Abstract
Objective
Arthroscopic ankle arthrodesis (AAA) is a standard surgical method for the treatment of advanced traumatic ankle arthritis and has become more popular due to its advantages. To fix the tibiotalar joint, the use of three percutaneous screws is considered to have better mechanical stability than the use of two screws. However, it is sometimes difficult to insert three screws because they might block each other due to the small area of the tibiotalar joint surface and the large diameter of the screws; few articles illustrate how to insert three screws without the screws disturbing each other. The purpose of this study is to explore possible screw configurations of tripod fixation in arthroscopic ankle arthrodesis that avoid the collision of screws and yield better biomechanical performance.
Methods
We used the finite element method to examine the impact of different screw positions and orientations on the biomechanical characteristics of a three-dimensional (3D) ankle model. Maximum and average micromotion, pressure on the articular surface, and von Mises stress values of the tibia and the talus were used to evaluate the initial stability of the ankle.
Results
Five kinds of three-screw configurations were identified, and finite element analysis results suggested that configurations with the posteromedial home-run screw presented lower micromotion (especially configuration 3) and better screw distributions on the articular surface than those with the posterolateral home-run screw.
Conclusion
Screw configurations with the posteromedial home-run screw avoid collision and are more biomechanically stable than those with the posterolateral home-run screw. Thus, inserting the home-run screw through the posteromedial approach is recommended for clinical practice.
Epstein-Barr virus and human adenovirus viremia in renal tumors is associated with histological features of kidney cancer
Authors: Kryst Piotr , Poletajew Sławomir , Wyczałkowska-Tomasik Aleksandra , Gonczar Stefan , Wysocki Maciej , Kapuścińska Renata , Zgliczyński Wojciech , Pączek Leszek 
Published: April 2020

Abstract
Background. There is a growing evidence that viral infections may impact the risk and clinical course of malignancies, including solid tumors. The aim of this study was to assess the possible association of selected chronic / latent viral infections with the clinical course of renal cell carcinoma (RCC).Methods. In this prospective study we enrolled 27 patients undergoing partial or radical nephrectomy due to the histologically confirmed RCC and followed them up for one year post-operation. Isolation of the nucleic acids of human adenovirus (ADV), herpes simplex virus HSV-1 and HSV-2, Epstein-Barr virus (EBV), cytomegalovirus (CMV), BK virus (BKV) and John Cunningham virus (JCV) from tumor tissue was performed using the NucleoSpin Tissue Kit (Macherey-Nagel, Düren, Germany) or EZ1 Virus Mini Kit (Qiagen, Hilden, Germany). The number of viral copies in the tissue was assessed with the real-time PCR.Results. Viral infections were diagnosed in ten patients (37.0%), including three ADV cases (11.1%) and eight EBV cases (29.6%). Infected patients tended to be significantly older (71.3 vs. 57.6 years, p < 0.05), more commonly presented with chronic renal disease (OR 2.4, p < 0.05), diabetes (OR 4.2, p < 0.05) and overweight (OR 2.0, p < 0.05). Regarding oncological data, infected patients were found to have a higher rate of high-grade cancers (OR 5.0, p < 0.05) and a higher rate of papillary RCCs (OR 8.3, p < 0.05). Status of viral infections had no influence on the clinical cancer stage, surgical procedure or survival.Conclusions. EBV and ADV infections are common in renal cancer patients and increase the risk of high-grade RCC presence. While there is no significant impact on short term survival, further studies are needed to assess the relevance of these findings in a long run.Trial registration. Medical University of Warsaw KB/37/2017
Platelet count as a biomarker for monitoring treatment response and disease recurrence in recurrent epithelial ovarian cancer
Authors: Hu Qinghong , Hada Abha , HAN Liping 
Published: April 2020

Abstract
Objectives
We sought to determine the impact of pretreatment plasma platelet levels, dimerized plasmin fragment (D-dimer) and fibrinogen in recurrent epithelial ovarian cancer (EOC) and the impact of platelet levels on SKOV3 cell lines growth and responsiveness to chemotherapy.
Methods
Under approval of ethical committee, we identified 104 women with recurrent EOC who underwent treatment between January 2010 and February 2015. Reviewing clinical, laboratory, and pathologic records from this retrospective cohort, we analyzed the correlation between pretreatment plasma D-dimer, fibrinogen, platelet levels and clinicopathological parameters, progression free survival (PFS)and overall survival (OS). Inco-culture experiments human ovarian cancer SKOV3 cell lines were used to test the effect of platelet levels on tumor growth and responsiveness to docetaxel.
Results
Of the 104 recurrent EOC, thrombocytosis at diagnosis and the decrease of platelet count by less than 25% after primary therapy were associated with worse median progression free survival (P = 0.003;P = 0.021) and median overall survival (P = 0.009;P = 0.009).Mean platelet levels declined at the end of primary therapy(P < 0.001) and rose at recurrence(P = 0.007) a.In multivariate analysis, elevated platelet levels at primary therapy and the decrease of platelet count less than 25% after primary therapy were unfavorable prognostic factor for PFS( P = 0.022; P = 0.015) and OS(P = 0.013;P = 0.007)in recurrent EOC, but elevated plasma D-dimer and fibrinogen were not. In SKOV-3 ovarian cancer cell lines, suitable concentration platelet co-culture protected against apoptosis (P < 0.05).
Conclusions
Platelet count during treatment could be used as a biomarker used for monitoring the disease recurrence and predicting treatment response. And platelet with suitable concentration co-culture protected against apoptosis inSKOV3 cell line, which may explain clinical observations.
Metabolomics Reveals Ecological Significance of Secondary Metabolites in Scutellaria baicalensis under drought stress
Authors: Meng Xiang-cai , Wang Bin , Cong Wei , Du Hongwei , Zhang Ai-hua , Wang Xi-jun 
Published: April 2020

Abstract
Background Plant have to face the more environmental stress than animal which can dodge unfavourable circumstances by moving about, it is inevitable that the reactive oxygen species(ROS), as a product of stress, are massive generated. Excessive ROS with a powerful oxidizability under stress could do harm to the protein, including enzymes. So it is impossible to eliminated too much ROS only by antioxidant enzymes. Plant have evolved a secondary metabolism as peculiar additional pathway, but the biosynthesis of secondary metabolites would be extremely costly due to the consumption of much material and energy during suitable condition, secondary metabolism are augmented only after the stress befall, meaning the secondary metabolites vary according to the ecological environment. Results Using UHPLC -ESI-Q-TOF-MS/MS analysis, a total of 24 differential compounts in root of Scutellaria baicalensis were identified between the drought and suitable condition. Based on Results of t-test analysis(P<0.05)between various groups, ions whose VIP value ≧2, the most significant differential chemical markers of the drought condition were citric acid, shikimic acid, baicalin, wogonoside, baicalein, wogonin, 3,5,7,2',6'-pentahydroxyflavanone, 5,2',6'-trihydroxy-7,8 -dimethoxyflavone, chrysin, eriodictyol, 5,8-dihydroxy-6,7-dimethoxy flavone, highlighting that most of them were free flavonoids with many phenolic hydroxyl group of flavonoids, with a characteristic of higher avtivities. Conclusions The diversity of secondary metabolites plays a crucial role, S. baicalensis modified the ability to eliminate ROS and matained the equilibrium of ROS through the biosynthesis and convertion between these flavonoid which contain many compounts, like an intricate buffer solution.
Correlation of Gut Firmicutes/Bacteroidetes Ratio with Fibrosis and Steatosis in Patients with Non-alcoholic Fatty Liver Disease
Authors: Jasirwan Chyntia Olivia Maurine , Muradi Akhmadu , Hasan Irsan , Simadibrata Marcellus , Rinaldi Ikhwan , Lesmana Cosmas Rinaldi A , Sulaiman Andri Sanityoso , Kurniawan Juferdy , Kalista Kemal Fariz , Nababan Saut Horas , Gani Rino Alvani 
Published: April 2020

Abstract
Background : We investigated the gut microbiota in patients with non-alcoholic fatty liver disease (NAFLD) and its correlation with fibrosis and steatosis as reflected in the controlled attenuation parameter and transient elastography valuesMethods : A cross-sectional study was performed on 37 patients with NAFLD at Cipto Mangunkusumo National General Hospital from December 2018 to March 2019. The gut microbiota was investigated in fecal samples with 16S RNA sequencing using the next-generation sequencing platform MiSeq (Illumina).Results : NAFLD was more common in patients with metabolic syndrome. Firmicutes, Bacteroidetes, and Proteobacteria were the predominant phyla. Bacteroides was more dominant than Prevotella, contrary to the results in previous studies on normal populations in Indonesia. Microbiota dysbiosis was observed in most samples. The gastrointestinal microbiota diversity was significantly decreased in patients with NAFLD with high triglyceride levels and central obesity. The Firmicutes/Bacteroidetes ratio correlated with steatosis and obesity, whereas some other species in the lower taxonomy were mostly correlated with steatosis and obesity without fibrosis. Proteobacteria is the only phylum strongly correlated with fibrosis in patients with normal body mass index.Conclusions : The gut microbiota diversity was decreased in patients with NAFLD with high triglyceride levels and central obesity, and certain gut microbes were correlated with fibrosis and steatosis.
In-silico Immunomodelling of 2019-nCoV
Authors: Maali Amirhosein , Teimouri Hossein , Amiri Shahin , Adibzadeh Setare , Azad Mehdi 
Published: April 2020

Abstract
Background: Novel Corona Virus 2019 (2019-nCoV) is a positive-sense single-strand RNA virus form coronaviridae family, responsible for corona virus infectious disease 2019 (COVID-19) with rapid transmission. The aim of this study is characterization of major viral proteins, prediction of antigen proteasomal cleavage pattern, MHC class I processing and presentation, B- and T-cell epitopes, and anti-inflammatory epitopes of 2019-nCoV, compared with SARS-CoV. Methods: The aminoacid sequence of spike surface (S) glycoprotein, membrane (M) glycoprotein, envelop (E) protein and nucleocapsid (N) phosphoprotein were obtained from NCBI. The sequences were aligned by MEGA 7.0 and modeled by SWISS-MODEL. The proteasomal cleavage pattern, MHC class I processing and T-cells epitopes were predicted via IEDB analysis and EPISOFT. The B-cell epitopes were predicted by BepiPred 2.0. Also, prediction of anti-inflammatory epitopes was performed by AntiFlam. Results: Two major antigen proteins, S glycoprotein and M glycoprotein of 2019-nCoV, respectively, have 26.57% and 20.59% less efficiency in proteasomal cleavage and presentation to MHC class I, comparing SARS-CoV. There are less B-cell predicted epitopes in 2019-nCoV, comparing SARS-CoV. The anti-inflammatory properties of 2019-nCoV S glycoprotein and N protein is higher than SARS-CoV. Discussion: It seems that the evolution of 2019-nCoV is on the way of deficiency in antigen presenting to MHC class I and escaping from cellular immunity. Also, the predicted hotspot epitopes potentially can be used to induction of adaptive cellular immunity against 2019-nCoV. In addition, 2019-nCoV appears to be less immunopathogenic than SARS-CoV due to its higher anti-inflammatory proteins.
Comprehensive Analysis of the Expression of Sodium/Potassium-ATPase α Subunits and Prognosis of Ovarian Serous Cystadenocarcinoma
Authors: Huang Wei , Zhang Yongjian , Xu Ye , Yang Shaoyou , Li Bing , Huang Lan , Lou Ge 
Published: April 2020

Abstract
Background
Ovarian serous cystadenocarcinoma (OSC) is the most common and lethal gynecological cancer in women worldwide. Biomarkers to diagnose and predict prognosis of OSC remain limited. We therefore aimed to investigate whether Na+/K+-ATPase α-subunits (ATP1As) are helpful diagnostic and prognostic markers of OSC.
Methods
Gene expression data (RNA-Seq) of 376 patients with ovarian serous cystadenocarcinoma were downloaded from The Cancer Genome Atlas (TCGA) program database. Further databases used in our analysis included Genotype-Tissue Expression (GTEx), the Human Protein Atlas, cBioPortal for Cancer Genomics, and Cancer Cell Line Encyclopedia (CCLE).
Results
Expression levels of ATP1A1 and ATP1A3 were higher in OSC tissues than in normal ovarian tissues, whereas the expression level of ATP1A2 and ATP1A4 were lower in the former than in the latter. Overexpression of ATP1A2 was significantly associated with higher Federation of Gynecology and Obstetrics (FIGO) stage and histologic grade. Increased mRNA expression of ATP1A3 was significantly associated with shorter overall survival (OS) and disease-specific survival (DSS) in OSC patients, while greater ATP1A4 levels were associated with favorable OS and DSS. Multivariate analysis also showed that primary therapy outcome, residual tumor, and mRNA expressions of ATP1A3 and ATP1A4 were independent prognostic factors for both OS and DSS of OSC patients. Moreover, ATP1A1 staining was abundant in tumor tissues. Furthermore, a high expression of ATP1A3 was significantly correlated with poor OS and DSS in the subgroup of patients aged ≥ 60 years, FIGO stage III, histological grade G3, and TP53 mutation. Mutation frequencies of the ATP1As ranged 3%-5%.
Conclusions
In conclusion, these results indicate that the ATP1As gene family could be potential diagnostic or prognostic markers for OSC. In addition, ATP1As may be effective therapeutic targets for the treatment of OSC.
Evaluation of an in-house indirect enzyme-linked immunosorbent assay of feline panleukopenia VP2 subunit antigen in comparison to hemagglutination inhibition assay to monitor tiger antibody levels by Bayesian approach
Authors: Areewong Chanakan , Rittipornlertrak Amarin , Nambooppha Boondarika , Fhaikrue Itsarapan , Singhla Tawatchai , Sodarat Chollada , Prachasilchai Worapat , Vongchan Preeyanat , Sthitmatee Nattawooti 
Published: April 2020

Abstract
Background: Feline panleukopenia virus (FPV) is an etiologic pathogen of feline panleukopenia that infects all members of Felidae including tigers (Panthera tigris). Vaccination against FPV among wild felid species has long been practiced in zoos worldwide. However, few studies have assessed tiger immune response post-vaccination due to the absence of a serological diagnostic tool. To address these limitations, this study aimed to develop an in-house indirect enzyme-linked immunosorbent assay (ELISA) for the monitoring of tiger antibody levels against the feline panleukopenia vaccine by employing the synthesized subunit capsid protein VP2. An in-house horseradish peroxidase (HRP) conjugated rabbit anti-tiger immunoglobulin G (IgG) polyclonal antibody (HRP-anti-tiger IgG) was produced in this study and employed in the assay. It was then compared to a commercial HRP-conjugated goat anti-cat IgG (HRP-anti-cat IgG). Sensitivity and specificity were evaluated using the Bayesian model with conditional dependence being assumed between both HRP-conjugated antibody-based ELISAs and hemagglutination-inhibition (HI) tests. Results: The posterior estimates for sensitivity and specificity of two indirect ELISA HRP-conjugated antibodies were higher than those of the HI test. The sensitivity and specificity of indirect ELISA for HRP-anti-tiger IgG and HRP-anti-cat IgG were 86.5%, 57.2% and 86.7%, 64.6%, respectively, while the results of the HI test were 79.1 and 54.1%. In applications, 89.6% (198/221) of tiger serum samples were determined to be seropositive by indirect ELISA testing. Conclusion: The results support evidence that an in-house indirect ELISA developed in this study could be used as a serological tool for the effective detection of tiger antibodies.
Liver damage in severe COVID-19 patients from Sichuan area
Authors: Li Tianlong , Deng Chao , Du Qing , Chen Hong , Lu Sen , Yue Ruiming , Guo Yang , Huang Xiaobo , Pan Lingai 
Published: April 2020

Abstract
Background: COVID-19 has spread worldwide, which becomes a huge threat to human beings.Materials: Severe COVID-19 patients from Sichuan area admitted to department of critical care medicine in Chengdu Public Health Clinical Medical Center were retrospectively enrolled. The liver function during the ICU hospitalization were record and analyzed.Results: The severe COVID-19 patients mainly presented with respiratory symptoms such as fever, cough and dyspnea, and the incidence was higher in the elderly patients and males. ALT, AST, TB, and PT increased to varying degrees during the course of the disease, and ALB decreased. The incidence of liver dysfunction in patients taking Lopinavir/Ritonavi was significantly higher than patient who did not have it, but there was no statistical difference (p<0.05). Patients taking low-dose Lopinavir/Ritonavi had a smaller effect on liver function than patients receiving normal dosage.Conclusion: Severe COVID-19 patients have obvious liver damage early in the course of the disease and have a slower recovery. Pay attention to avoid using drugs that can aggravate liver damage while treating the primary disease. If there is no alternative drug, we can give some liver protection treatment appropriately.
Joint force of individual, hospital, government: Simulation of the spread of 2019 novel coronavirus by multi-agents complex adaptive system
Authors: Zhong Weiqiong , Wang Tianxiu 
Published: April 2020

Abstract
Early forecasting is important for health officials and decision-makers to respond to public health emergencies such as the 2019 novel coronavirus (COVID-19) outbreak. The spread of epidemic is mainly impacted by the interaction among agents, i.e., individual, hospital and government. In an effort to efficiently mitigate the impact of the virus, this original study designed a multi-agents complex adaptive system model of COVID-19 to detect the trend of the spread in 8 countries by simulating the adaptation and interaction among the agents. The results show that there should be 127,726 infections in China, 12,000 in South Korea, 729,377 in the USA, 176,623 in Italy, 194,359 in Spain, 165,122 in Germany, 177,462 in France and 149,540 in UK. It is impossible to screen and report all the cases, and the detection rate may be 82% in Italy, 65% in China, 55% in the USA, and 41% in UK on April 8, 2020. Scenario simulation results imply that action time is the most important factor in containing the spread of epidemic. If China had locked down Wuhan city five days later, the total infection would be tripled. The forces of individual, hospital and government should be united in fighting the virus.