bluuebunny/crossref_metadata_2025_split huggingface dataset:
Heterobilayers of 2D materials as a platform for excitonic superfluidity
Authors: Gupta Sunny , Kutana Alex , Yakobson Boris I. 
Published: June 2020

AbstractExcitonic condensate has been long-sought within bulk indirect-gap semiconductors, quantum wells, and 2D material layers, all tried as carrying media. Here, we propose intrinsically stable 2D semiconductor heterostructures with doubly-indirect overlapping bands as optimal platforms for excitonic condensation. After screening hundreds of 2D materials, we identify candidates where spontaneous excitonic condensation mediated by purely electronic interaction should occur, and hetero-pairs Sb2Te2Se/BiTeCl, Hf2N2I2/Zr2N2Cl2, and LiAlTe2/BiTeI emerge promising. Unlike monolayers, where excitonic condensation is hampered by Peierls instability, or other bilayers, where doping by applied voltage is required, rendering them essentially non-equilibrium systems, the chemically-specific heterostructures predicted here are lattice-matched, show no detrimental electronic instability, and display broken type-III gap, thus offering optimal carrier density without any gate voltages, in true-equilibrium. Predicted materials can be used to access different parts of electron-hole phase diagram, including BEC-BCS crossover, enabling tantalizing applications in superfluid transport, Josephson-like tunneling, and dissipationless charge counterflow.
Non-thermal resistive switching in Mott insulator nanowires
Authors: Kalcheim Yoav , Camjayi Alberto , del Valle Javier , Salev Pavel , Rozenberg Marcelo , Schuller Ivan K. 
Published: June 2020

AbstractResistive switching can be achieved in a Mott insulator by applying current/voltage, which triggers an insulator-metal transition (IMT). This phenomenon is key for understanding IMT physics and developing novel memory elements and brain-inspired technology. Despite this, the roles of electric field and Joule heating in the switching process remain controversial. Using nanowires of two archetypal Mott insulators—VO2 and V2O3 we unequivocally show that a purely non-thermal electrical IMT can occur in both materials. The mechanism behind this effect is identified as field-assisted carrier generation leading to a doping driven IMT. This effect can be controlled by similar means in both VO2 and V2O3, suggesting that the proposed mechanism is generally applicable to Mott insulators. The energy consumption associated with the non-thermal IMT is extremely low, rivaling that of state-of-the-art electronics and biological neurons. These findings pave the way towards highly energy-efficient applications of Mott insulators.
Adipocyte Gi signaling is essential for maintaining whole-body glucose homeostasis and insulin sensitivity
Authors: Wang Lei , Pydi Sai P. , Zhu Lu , Barella Luiz F. , Cui Yinghong , Gavrilova Oksana , Bence Kendra K. , Vernochet Cecile , Wess Jürgen 
Published: June 2020

AbstractAdipocyte dysfunction links obesity to insulin resistance and type 2 diabetes. Adipocyte function is regulated by receptor-mediated activation of heterotrimeric G proteins. Little is known about the potential in vivo metabolic roles of Gi-type G proteins expressed by adipocytes, primarily due to the lack of suitable animal models. To address this question, we generated mice lacking functional Gi proteins selectively in adipocytes. Here we report that these mutant mice displayed significantly impaired glucose tolerance and reduced insulin sensitivity when maintained on an obesogenic diet. In contrast, using a chemogenetic strategy, we demonstrated that activation of Gi signaling selectively in adipocytes greatly improved glucose homeostasis and insulin signaling. We also elucidated the cellular mechanisms underlying the observed metabolic phenotypes. Our data support the concept that adipocyte Gi signaling is essential for maintaining euglycemia. Drug-mediated activation of adipocyte Gi signaling may prove beneficial for restoring proper glucose homeostasis in type 2 diabetes.
3D sedimentary architecture showing the inception of an Ice Age
Authors: Løseth H. , Dowdeswell J. A. , Batchelor C. L. , Ottesen D. 
Published: June 2020

AbstractNortheast Atlantic climate shifted into the Quaternary Ice Age around 2.6 M yr ago. Until now, however, the detailed changes associated with this inception of an Ice Age have remained obscure. New high-quality three-dimensional seismic data reveal a detailed geological record of buried surfaces, landforms and sedimentary architecture over vast parts of the Norwegian North Sea. Here, we show the sequence of near-coast geological events spanning the Northeast Atlantic inception of an Ice Age. We identify the location of immediate pre-glacial fluvially derived sandy systems where rivers from the Norwegian mainland built marine deltas. The stratigraphic position of a large submarine channel, formed by enhanced meltwater from initial build-up of local glaciers, is also shown. Finally, we document the transition to full ice-sheet growth over Scandinavia from the ice sheet’s earliest position to the later pattern of debris-flow lobes reaching the present-day shelf edge.
CRISPR artificial splicing factors
Authors: Du Menghan , Jillette Nathaniel , Zhu Jacqueline Jufen , Li Sheng , Cheng Albert Wu 
Published: June 2020

AbstractAlternative splicing allows expression of mRNA isoforms from a single gene, expanding the diversity of the proteome. Its prevalence in normal biological and disease processes warrant precise tools for modulation. Here we report the engineering of CRISPR Artificial Splicing Factors (CASFx) based on RNA-targeting CRISPR-Cas systems. We show that simultaneous exon inclusion and exclusion can be induced at distinct targets by differential positioning of CASFx. We also create inducible CASFx (iCASFx) using the FKBP-FRB chemical-inducible dimerization domain, allowing small molecule control of alternative splicing. Finally, we demonstrate the activation of SMN2 exon 7 splicing in spinal muscular atrophy (SMA) patient fibroblasts, suggesting a potential application of the CASFx system.
Oculomotor behavior tracks the effect of ideological priming on deception
Authors: Schepisi Michael , Porciello Giuseppina , Aglioti Salvatore Maria , Panasiti Maria Serena 
Published: June 2020

AbstractThe decision to lie to another person involves a conflict between one’s own and others’ interest. Political ideology may foster self-promoting or self-transcending values and thus may balance or fuel self vs. other related conflicts. Here, we explored in politically non-aligned participants whether oculomotor behavior may index the influence on moral decision-making of prime stimuli related to left and right-wing ideologies. We presented pictures of Italian politicians and ideological words in a paradigm where participants could lie to opponents with high vs. low socio-economic status to obtain a monetary reward. Results show that left-wing words decreased self-gain lies and increased other-gain ones. Oculomotor behavior revealed that gazing longer at politicians’ pictures led participants to look longer at opponent’s status-related information than at game’s outcome-related information before the decision. This, in turn, caused participants to lie less to low status opponents. Moreover, after lying, participants averted their gaze from high status opponents and maintained it towards low status ones. Our results offer novel evidence that ideological priming influences moral decision-making and suggest that oculomotor behavior may provide crucial insights on how this process takes place.
Knowledge, attitude and practice on dengue prevention and dengue seroprevalence in a dengue hotspot in Malaysia: A cross-sectional study
Authors: Selvarajoo Sivaneswari , Liew Jonathan Wee Kent , Tan Wing , Lim Xin Ying , Refai Wardha F. , Zaki Rafdzah Ahmad , Sethi Neha , Wan Sulaiman Wan Yusoff , Lim Yvonne Ai Lian , Vadivelu Jamuna , Vythilingam Indra 
Published: June 2020

AbstractDengue has become a global public health problem. Despite reactive efforts by the government in Malaysia, the dengue cases are on the increase. Adequate knowledge, positive attitude and correct practice for dengue control are essential to stamp out the disease. Hence, this study aims to assess the factors associated with dengue knowledge, attitude and practice (KAP), as well as the association with dengue IgM and IgG seropositivity. A community-based cross-sectional study was conducted in a closed, dengue endemic area with multi-storey dwellings . Five hundred individuals (aged 18 years and above) were approached for pre-tested KAP and seroprevalences assessment. The study showed only half of the total participants have good knowledge (50.7%) but they had insufficient knowledge about dengue during pregnancy. 53.2% of people had poor attitude and 50.2% reported poor practice for dengue control. Out of 85 respondents who agreed to participate in the dengue seroprevalence study, 74.1% (n = 63) were positive for dengue IgG and 7.1% (n = 6) were positive for dengue IgM. Among all sociodemographic variable, race is the only independent predicator for all KAP levels (P < 0.05). In conclusion, proactive and sustainable efforts are needed to bring a behavioural change among communities in order to fight dengue outbreaks in endemic areas.
Increasing incidence of group B streptococcus neonatal infections in the Netherlands is associated with clonal expansion of CC17 and CC23
Authors: Jamrozy Dorota , Bijlsma Merijn W. , de Goffau Marcus C. , van de Beek Diederik , Kuijpers Taco W. , Parkhill Julian , van der Ende Arie , Bentley Stephen D. 
Published: June 2020

AbstractGroup B streptococcus (GBS) is the leading cause of neonatal invasive disease worldwide. In the Netherlands incidence of the disease increased despite implementation of preventive guidelines. We describe a genomic analysis of 1345 GBS isolates from neonatal (age 0–89 days) invasive infections in the Netherlands reported between 1987 and 2016. Most isolates clustered into one of five major lineages: CC17 (39%), CC19 (25%), CC23 (18%), CC10 (9%) and CC1 (7%). There was a significant rise in the number of infections due to isolates from CC17 and CC23. Phylogenetic clustering analysis revealed that this was caused by expansion of specific sub-lineages, designated CC17-A1, CC17-A2 and CC23-A1. Dating of phylogenetic trees estimated that these clones diverged in the 1960s/1970s, representing historical rather than recently emerged clones. For CC17-A1 the expansion correlated with acquisition of a new phage, carrying gene encoding a putative cell-surface protein. Representatives of CC17-A1, CC17-A2 and CC23-A1 clones were identified in datasets from other countries demonstrating their global distribution.
Visuomotor deficiency in panx1a knockout zebrafish is linked to dopaminergic signaling
Authors: Safarian Nickie , Whyte-Fagundes Paige , Zoidl Christiane , Grigull Jörg , Zoidl Georg 
Published: June 2020

AbstractPannexin 1 (Panx1) forms ATP-permeable membrane channels that play roles in the nervous system. The analysis of roles in both standard and pathological conditions benefits from a model organism with rapid development and early onset of behaviors. Such a model was developed by ablating the zebrafish panx1a gene using TALEN technology. Here, RNA-seq analysis of 6 days post fertilization larvae were confirmed by Real-Time PCR and paired with testing visual-motor behavior and in vivo electrophysiology. Results demonstrated that loss of panx1a specifically affected the expression of gene classes representing the development of the visual system and visual processing. Abnormal swimming behavior in the dark and the expression regulation of pre-and postsynaptic biomarkers suggested changes in dopaminergic signaling. Indeed, altered visuomotor behavior in the absence of functional Panx1a was evoked through D1/D2-like receptor agonist treatment and rescued with the D2-like receptor antagonist Haloperidol. Local field potentials recorded from superficial areas of the optic tectum receiving input from the retina confirmed abnormal responses to visual stimuli, which resembled treatments with a dopamine receptor agonist or pharmacological blocking of Panx1a. We conclude that Panx1a functions are relevant at a time point when neuronal networks supporting visual-motor functions undergo modifications preparing for complex behaviors of freely swimming fish.
An AgNP-deposited commercial electrochemistry test strip as a platform for urea detection
Authors: Liu Juanjuan , Siavash Moakhar Roozbeh , Sudalaiyadum Perumal Ayyappasamy , Roman Horia Nicolae , Mahshid Sara , Wachsmann-Hogiu Sebastian 
Published: June 2020

AbstractWe developed an inexpensive, portable platform for urea detection via electrochemistry by depositing silver nanoparticles (AgNPs) on a commercial glucose test strip. We modified this strip by first removing the enzymes from the surface, followed by electrodeposition of AgNPs on one channel (working electrode). The morphology of the modified test strip was characterized by Scanning Electron Microscopy (SEM), and its electrochemical performance was evaluated via Cyclic Voltammetry (CV) and Electrochemical Impedance Spectroscopy (EIS). We evaluated the performance of the device for urea detection via measurements of the dependency of peak currents vs the analyte concentration and from the relationship between the peak current and the square root of the scan rates. The observed linear range is 1–8 mM (corresponding to the physiological range of urea concentration in human blood), and the limit of detection (LOD) is 0.14 mM. The selectivity, reproducibility, reusability, and storage stability of the modified test strips are also reported. Additional tests were performed to validate the ability to measure urea in the presence of confounding factors such as spiked plasma and milk. The results demonstrate the potential of this simple and portable EC platform to be used in applications such as medical diagnosis and food safety.
Bidirectional association between asthma and chronic rhinosinusitis: Two longitudinal follow-up studies using a national sample cohort
Authors: Ryu Gwanghui , Min Chanyang , Park Bumjung , Choi Hyo Geun , Mo Ji-Hun 
Published: June 2020

AbstractThe aim of this study was to evaluate an epidemiologic association of asthma and chronic rhinosinusitis (CRS) using a national sample cohort of the Korean population. We collected data from the Korean Health Insurance Review and Assessment Service-National Sample Cohort between 2002 and 2013, and two different case-control cohorts were designed (1st: asthmatic patients matched in a 1:1 ratio with 204,119 non-asthmatics as control I, 2nd: CRS patients matched in a 1:4 ratio with 124,020 non-CRS patients as control II). Bidirectional association was examined using Cox proportional hazard models stratified by age, sex, income, and region of residence. Patients with asthma had an increased risk of developing CRS [adjusted hazard ratio (95% confidence interval) = 1.74 (1.67–1.80)], both with nasal polyps [1.55 (1.36–1.78)], without nasal polyps [1.74 (1.67–1.81)]. In the second cohort, patients with CRS had increased risk of developing asthma [1.85 (1.80–1.91)] with similar results for those with and without nasal polyps. The strongest association for risk of CRS was in 20–39 years old men with asthma [2.41 (1.97–2.96)], while the strongest association for increased risk of asthma in those with CRS group was also seen in this same subgroup [2.40 (2.18–2.63)]. CRS and asthma had a bidirectional influence on each other. CRS increased the risk of asthma, and asthma increased the risk of CRS, especially in young men.
Crystal structures of Uso1 membrane tether reveal an alternative conformation in the globular head domain
Authors: Heo Yoonyoung , Yoon Hye-Jin , Ko Hanseo , Jang Soonmin , Lee Hyung Ho 
Published: June 2020

AbstractMembrane tethers play a critical role in organizing the complex molecular architecture of eukaryotic cells. Uso1 (yeast homolog of human p115) is essential for tethering in vesicle transport from ER to Golgi and interacts with Ypt1 GTPase. The N-terminal globular head domain of Uso1 is responsible for Ypt1 binding; however, the mechanism of tethering between ER transport vesicles and Golgi is unknown. Here, we determined two crystal structures for the Uso1 N-terminal head domain in two alternative conformations. The head domain of Uso1 exists as a monomer, as confirmed using size-exclusion chromatography coupled to multi-angle light scattering and analytical gel filtration. Although Uso1 consists of a right-handed α-solenoid, like that in mammalian homologs, the overall conformations of both Uso1 structures were not similar to previously known p115 structures, suggesting that it adopts alternative conformations. We found that the N- and C-terminal regions of the Uso1 head domain are connected by a long flexible linker, which may mediate conformational changes. To analyse the role of the alternative conformations of Uso1, we performed molecular docking of Uso1 with Ypt1, followed by a structural comparison. Taken together, we hypothesize that the alternative conformations of Uso1 regulate the precise docking of vesicles to Golgi.
First Principles Calculation for Photocatalytic Activity of GaAs Monolayer
Authors: Rouzhahong Yilimiranmu , Wushuer Mariyemu , Mamat Mamatrishat , Wang Qing , Wang Qian 
Published: June 2020

AbstractSolar energy hydrogen production is one of the best solutions for energy crisis. Therefore, finding effective photocatalytic materials that are able to split water under the sunlight is a hot topic in the present research fields. In addition, theoretical prediction is a present low-cost important method to search a new kind of materials. Herein, with the aim of seeking efficient photocatalytic material we investigated the photocatalytic activity of GaAs monolayer by the first principles calculation. According to the obtained electronic and optical properties, we primarily predicted the photocatalytic water splitting activity of GaAs monolayer, which the result further confirmed by the calculated reaction free energy. More remarkably, predicted carrier mobility of GaAs monolayer 2838 cm2V−1s−1is higher than 200 cm2V−1s−1of MoS2. Our finding provides a promising material for the development of renewable energy conversion and a new outlook for better designing of a superior photocatalyst for water splitting.
Metabolic reprogramming related to whole-chromosome instability in models for Hürthle cell carcinoma
Authors: Addie Ruben D. , Kostidis Sarantos , Corver Willem E. , Oosting Jan , Aminzadeh-Gohari Sepideh , Feichtinger René G. , Kofler Barbara , Aydemirli Mehtap Derya , Giera Martin , Morreau Hans 
Published: June 2020

AbstractHürthle cell carcinoma (HCC) is a recurrent subtype of non-medullary thyroid cancer. HCC is characterized by profound whole-chromosome instability (w-CIN), resulting in a near-homozygous genome (NHG), a phenomenon recently attributed to reactive oxygen species (ROS) generated during mitosis by malfunctioning mitochondria. We studied shared metabolic traits during standard and glucose-depleted cell culture in thyroid cancer cell lines (TCCLs), with or without a NHG, using quantitative analysis of extra and intracellular metabolites and ROS production following inhibition of complex III with antimycin A. We found that the XTC.UC1 and FTC-236 cell lines (both NHG) are functionally impaired in complex I and produce significantly more superoxide radicals than SW579 and BHP 2–7 (non-NHG) after challenge with antimycin A. FTC-236 showed the lowest levels of glutathione and SOD2. XTC.UC1 and FTC-236 both exhibited reduced glycolytic activity and utilization of alternative sources to meet energy demands. Both cell lines also shared low levels of α-ketoglutarate and high levels of creatine, phosphocreatine, uridine diphosphate-N-acetylglucosamine, pyruvate and acetylcarnitine. Furthermore, the metabolism of XTC.UC1 was skewed towards the de novo synthesis of aspartate, an effect that persisted even in glucose-free media, pointing to reductive carboxylation. Our data suggests that metabolic reprogramming and a subtle balance between ROS generation and scavenging/conversion of intermediates may be involved in ROS-induced w-CIN in HCC and possibly also in rare cases of follicular thyroid cancer showing a NHG.
The development of a scoring and ranking strategy for a patient-tailored adverse drug reaction prediction in polypharmacy
Authors: Valeanu Andrei , Damian Cristian , Marineci Cristina Daniela , Negres Simona 
Published: June 2020

AbstractOnly few applications are currently dealing with personalized adverse drug reactions (ADRs) prediction in case of polypharmacy. The study aimed to develop a patient-tailored ADR web application, considering characteristics from 734 drugs and relevant patient related factors. The application was designed in Python using a scoring and ranking system based on frequency and severity, computed for each ADR and expressed through an online platform. A neural networks algorithm was used for predicting the severity of ADRs. The application inputs are: age, gender, drugs, relevant pathologies. The outputs are: an overall severity profile (hospitalization and mortality risk), a stratified risk on specific ADR groups and a sorted list of the most important ADRs depending on frequency and severity. The Severity prediction model validation resulted in 79.7–85.1% Area Under the Receiver Operating Characteristic Curve Score, which lies in the good cut-off of 75–90%. The program offers a complex view regarding the ADR profile of a given patient and could be used by the physician and clinical pharmacist during patient safety monitoring, for a coherent therapy choice or medication adjustment, due to the good therapy coverage and the inclusion of relevant patient comorbidities.
Increased organic fertilizer application and reduced chemical fertilizer application affect the soil properties and bacterial communities of grape rhizosphere soil
Authors: Wu Linnan , Jiang Yu , Zhao Fengyun , He Xiufeng , Liu Huaifeng , Yu Kun 
Published: June 2020

AbstractIncreasing organic fertilizer application can improve the sustainability of soil productivity, but the effects of increased organic fertilizer application with reduced chemical fertilizer application over different time periods on chemical properties and bacterial community of grape rhizosphere soil in an arid region are not clear. In this study, three years of fixed-point field tests were used to compare the effects of various fertilization treatments on the soil properties and bacterial community in the grape rhizosphere. The results showed that (1) T1 and T2 significantly increased SOM, AN, AP and AK contents in grape rhizosphere soil. TN, TP and TK contents in grape leaves of T2 were the highest of those in five fertilization treatments. (2) The abundances of Proteobacteria and Bacteroidetes phyla and especially of Arthrobacter, Pseudomonas, Nitrosopira and Bacillus genera were higher in T2 than in the other samples. (3) SOM, AP and AN contents in soil were the main factors affecting soil bacterial community and mineral element contents in grape leaves and roots according to an RDA analysis. In summary, the application of organic fertilizer with reduced chemical fertilizer for two years had the greatest impact on the soil properties and bacterial community of the grape rhizosphere soil.