bluuebunny/crossref_metadata_2025_split huggingface dataset:
Predictive Factors of Outcome Following Non-Fermenting Gram-Negative Bacilli Peritonitis in Peritoneal Dialysis
Authors: Santos Ana Claudia Moro Lima dos , Hernandes Rodrigo Travanelli , Montelli Augusto Cezar , Monteiro Aydir Cecília Marinho , Barbosa Thais Alves , Camargo Carlos Henrique , Ferreira Adriano Martison , Mondelli Alessandro Lia , Cunha Maria de Lourdes Ribeiro de Souza da , BARRETTI PASQUAL 
Published: May 2020

Abstract
Background Peritonitis due to Gram-negative bacilli (GNB), particularly the non-fermenting GNB (NF-GNB) is a serious complication of peritoneal dialysis (PD) with a low-resolution rate. Beyond the patient’s conditions, microbiological properties as antimicrobial resistance, biofilm and other virulence factors production could explain the bad outcomes. This study aimed to evaluate the influence of the patient’s conditions, microbiological characteristics, including biofilm production, and treatment of peritonitis on the peritonitis resolution. Methods We reviewed the records of 68 peritonitis episodes that occurred between 1997 and 2015 at a single university center. The influence of microbiological and clinical factors on resolution chance was analyzed by logistic regression. Results The etiologies were species of Pseudomonas (51.5%), Acinetobacter (30.9%), and others (18.6%). There was a high (75%) proportion of biofilm producers’ lineages. The in vitro susceptibility rate of Pseudomonas spp. to amikacin, ciprofloxacin, and ceftazidime was significantly greater than that of Acinetobacter spp. and other species; however, there was a similar low-resolution rate (<40%) among the episodes due to these etiologies. Concomitant ESI and resistance to amikacin were independently associated with non-resolution. No other factor, including biofilm production were associated with the outcome Conclusions Peritonitis due to NF-GNB in PD are severe infections with reduced resolution rate; bacterial resistance and concomitant ESI negatively influence the chance of resolution. Even though biofilm production has not had influence the outcome, the found of higher in vitro susceptibility of Pseudomonas than other NF-GNB, with similar resolution rate, suggest bacterial virulence factors as biofilm or others can act in concert, worsening the outcome.
A cross-sectional study on metabolic similarities and differences between inpatients with schizophrenia and those with mood disorders
Authors: Uju Yoriyasu , Kanzaki Tetsuto , Yamasaki Yuki , Kondo Tadayuki , Nanasawa Hideki , Takeuchi Yu , Yanagisawa Yuta , Kusanishi Shun , Nakano Chieko , Enomoto Tetsuro , Sako Akahito , Yanai Hidekazu , Mishima Shunichi , Mimori Seisuke , Igarashi Kazuei , Takizawa Tsuyoshi , Hayakawa Tatsuro 
Published: May 2020

Abstract
Background
One of the main causes of death in psychiatric patients is cardiovascular diseases which are closely related with lifestyle-related diseases. Psychiatric disorders include schizophrenia and mood disorders, whose symptoms and treatment medicines are different, suggesting that they might have different metabolic disorders. Thus, we studied the differences of lifestyle-related diseases between schizophrenia and mood disorders in Japan.
Methods
This cross-sectional study was performed from 2015 to 2017. Study participants were 189 hospitalized patients (144 schizophrenia group, 45 mood disorders group) in the department of psychiatry at Kohnodai hospital. We examined physical disorders, metabolic status of glucose and lipid, estimated glomerular filtration rate (eGFR) and brain magnetic resonance imaging. We compared these data between inpatients with schizophrenia or mood disorders group and the standard. We quoted ‘The National Health and Nutrition Survey in Japan 2015’ by Ministry of Health, Labor and Welfare as the standard. In comparisons between schizophrenia and mood disorders groups, we used analysis of covariance or logistic regression analysis.
Results
The ratio of silent brain infarction (SBI) and cerebral infarction were significantly high in both groups compared with the standard. Schizophrenia group showed significantly higher prevalence of diabetes, low high-density lipoprotein (HDL) cholesterolemia, metabolic syndrome and smoking than the standard. Mood disorders group had significantly high prevalence of low HDL-cholesterolemia compared with the standard. Fasting blood glucose and HbA1c were significantly higher in schizophrenia group and female mood disorders group than the standard. Female mood disorders group had significantly decreased eGFR with increased ratio of eGFR < 60 ml/min than the standard. In comparison between both groups, eGFR and prevalence of smoking in mood disorders group were significantly lower than those in schizophrenia group by adjustment for age.
Conclusions
Participants of both groups had increased ratio of SBI and cerebral infarction, accompanied with glucose and lipid disorders. Distinct from schizophrenia group, mood disorders group showed significantly lower eGFR and prevalence of smoking.
The Establishment of Intervertebral Endplate Degeneration: A Novel in-vivo Diurnal-Axial Controllable Loading Model in Rat Tail
Authors: Hu Zhouyang , He Fan , Li Xinhua , Jiang Bei , Yan Shuaifeng , Tan Jun , Yang Mingjie , Li Lijun 
Published: May 2020

Abstract
Background. Cartilage endplate (CEP) acts as an important mechanical barrier and nutrient channel for the intervertebral disc (IVD). Its vulnerability to excessive loading and subsequent degeneration might be an initial trigger for IVD degeneration. This study aims to build a new in-vivo animal model of rat-tail CEP degeneration through implementing a controllable axial compressive device.Methods. An axial controllable compressive load was conducted on the 8–9 th coccygeal IVD of adult rat tail through puncturing 4 Kirschner wires into its superior and inferior vertebra. The exact compression loads were controlled and adjusted by a remote controller. The compression and decompression periods were set as 8 h/16 h per day. The control group was set as no device installation, while the Kirschner wires were punctured without compression in the sham group. After each 4, 8 and 12 weeks, 3 rats in each group were sacrificed and compressed disc were analyzed.Results. After compression, the targeted CEP degenerated gradually with time. Through 12 weeks' loading, the CEP structure collapsed completely and the Schmorl's nodes formed and penetrated from the cartilage layer into the vertebra. When compared with the control group, the number of micro porosities in the CEP becomes extremely scarce with loading, while the content of aggrecan and collagen II decreased significantly. However, these significant changes did not show up when sham and control groups were compared.Conclusions. A new excessive force induced CEP degeneration animal model was developed with its contribution as a practical alternative for further studying mechanisms of CEP degeneration.
Griseococcin(1) from Bovistella radicata (Mont.) Pat and Antimicrobial Activity
Authors: qingmei zeng , ye yong , Zeng Qinghua 
Published: May 2020

Abstract
Bovistella radicata (Mont.) Pat was a natural plant with multiple medicinal values. The purified compound purified from Bovistella radicata (Mont.) Pat by DEAE-cellulose and sephadex LC-20 column which could inhibit main pathogens leading to tinea pedis. Based on the spectral (HPLC, FT-IR, 1D and 2D NMR etc.) studies, purified compounds were identified as the Griseococcin(1) which were naphthoquinone derivatives, the Chemical formula and MW of Griseococcin(1) was determined as C37O10H43N and 661 Da. Minimum inhibitory concentration(MIC) and zone of inhibition(ZOI) of Griseococcin(1) were 31.2, 31.2 µg/ml and 18.06 ± 0.85 mm, 15.01 ± 1.02 mm respectively against main pathogenic fungus Trichophyton rubrum and Trichophyton mentagrophytes.
GDF15-Mediated Cholesterol Metabolism Promotes EMT and Metastasis in Esophageal Cancer
Authors: Dong Gang , Huang Xiaoquan , Jiang Siyu , Ni Liyuan , Ma Lili , Zhu Chouwen , Chen Shiyao 
Published: May 2020

Abstract
Background: GDF15 is a potential biomarker for patients with esophageal cancer (EC). However, the mechanistic role of GDF15 in the invasion and metastasis of EC remains poorly understood. Methods: We determined the expression and function of GDF15 in esophageal cancer cells (ESCCs) and in patient tissue samples using western blotting, migration and invasion assays, immunohistochemistry, Co-IP assays, and quantitative real-time-PCR. In addition, a pulmonary metastatic nude mouse model was used to determine the function of GDF15. We then supplemented our experimental results with database analysis to validate our findings.Results: GDF15 was upregulated in EC, and was associated with poor differentiation, high metastasis rates and worse prognosis. GDF15 knock-down reduced the migration and invasion of ESCCs. Co-IP assays demonstrated its association with SCAP, while GDF15 knock-down decreased SCAP levels. SCAP overexpression reversed the migration, invasion and EMT in GDF15-siRNA ESCCs. However, after incubation with β-cyclodextrin (β-CD), the ability of migration and invasion was weakened, EMT was reversed again. Migration, invasion, and EMT were enhanced in GDF15-siRNA ESCCs cultured in the presence of cholesterol and were similar to GDF15-siRNA ESCCs overexpressing SCAP. In vivo, knockdown of GDF15 inhibited lung metastasis of ESCCs and was reversed by SCAP overexpression or high cholesterol diet. Increased lung metastasis after SCAP overexpression was partially suppressed by intraperitoneal injection of β-CD. In addition, we determined that GDF15 was a direct target of miR-1324, miR-1324 was down-regulated in EC tissues. MiR-1324 upregulation resulted in decreased GDF15 expression and metastasis in ESCCs. Conclusions: We demonstrated that SCAP mediated GDF15-induced the invasion and metastasis of EC by regulating cholesterol metabolism. In addition, GDF15 was shown to be a direct target of miR-1324.
E2F1-mediated repression of WNT5A expression Promotes Brain Metastasis Dependent on the ERK1/2 pathway in EGFR-Mutant Non-Small Cell Lung Cancer
Authors: Li Huanhuan , Tong Fan , Meng Rui , Peng Ling , Zhang Ruiguang , Dong xiaorong 
Published: May 2020

Abstract
Background: Brain metastasis (BM) is associated with poor prognosis in patients with advanced non-small cell lung cancer (NSCLC). Epidermal growth factor receptor (EGFR) mutation reportedly enhances the development of BM. However, the exact mechanism of how EGFR-mutant NSCLC contributes to BM remains unknown. This study was aimed at exploring the mechanism of BM development in EGFR-mutant NSCLC.Methods: WNT5A, was identified by analyzing RNA sequencing data of BM tissue from NSCLC. The expression of WNT5A in NSCLC plasma (n=94) and cells were detected by quantitative real-time PCR (qRT-PCR) and western blotting. WNT5A functions were examined by cell viability, migration, invasion, and immunohistochemistry assay in vitro. A xenograft nude mouse model and BM model were used to observe tumor growth and brain metastasis in vivo. The potential transcription factor of WNT5A was explored using bioinformatics analysis and verified by chromatin immunoprecipitation (ChIP) and luciferase reporter assays. WNT5A targets in NSCLC cells were confirmed using luciferase reporter assay, qRT-PCR, and western blotting.Results: WNT5A was downregulated in BM tissues and EGFR-mutant samples and cells. The overexpression of WNT5A inhibited the growth, migration, and invasion of EGFR-mutant cells in vitro and retarded tumor growth and metastasis in vivo compared to the EGFR wide-type cells. ChIP and luciferase reporter assays showed that E2F1 negatively regulated WNT5A at transcriptional levels, which was suppressed by ERK1/2 inhibitor (U0126) in EGFR-mutant cells. Furthermore, WNT5A inhibited β-catenin activity and the transcriptional levels of its downstream genes in cancer progression.Conclusions: Our research revealed the role of WNT5A in NSCLC BM with EGFR mutation and proved that E2F1-mediated repression of WNT5A was dependent on the ERK1/2 pathway, supporting the notion that targeting the ERK1/2-E2F1-WNT5A pathway could be an effective strategy for treating BM in EGFR-mutant NSCLC.
The pathological significance of LOXL2 in pre-metastatic niche formation of HCC and its related molecular mechanism
Authors: Wu Sifan , Xing Xiaoxia , Wang Yaohui , Zhang Xi , Li Miao , Wang Mimi , Wang Zhiming , Chen Jie , Gao Dongmei , Zhao Yan , Chen Rongxin , Ren Zhenggang , Zhang Kezhi , Cui Jiefeng 
Published: May 2020

Abstract
Background: The mechanisms underlying the contribution of primary tumor to pre-metastatic niche formation remains largely unknown in HCC. We previously reported that the released Lysyl oxidase like 2 (LOXL2) from HCC cells under higher stiffness stimulation facilitated the formation of lung pre-metastatic niche. Here we further clarified the pathological role of LOXL2 in promoting lung pre-metastatic niche formation in HCC and its relevant molecular mechanism. Methods: Lung pre-metastatic niche nude mouse model and LOXL2-overexpressed xenograft HCC models were respectively developed to validate the significance of LOXL2 in pre-metastatic niche formation and pulmonary metastasis. Simultaneously, a gel-based cell culture system mirroring lung tissue stiffness in normal and pathological state was constructed to investigate the underlying mechanism of LOXL2-induced pre-metastatic niche formation.Results: LOXL2 by tail vein injection had obvious inducting effects on CD11b+/CD45+ BMDCs recruitment and fibronectin expression in lung tissue during the development of lung pre-metastatic niche. Tumor-secreted LOXL2 also promoted the occurrence of pulmonary metastasis and the number of metastasis lesions remarkably in LOXL2-overexpressed xenograft HCC models. Applying a gel-based cell culture system, we discovered that LOXL2 and LOXL2-caused matrix stiffening all upregulated the expressions of MMP9 and fibronectin in lung fibroblasts significantly, but little effect on MMP2 expression. Moreover, the activation of PI3K-AKT pathway participated in the regulation of LOXL2-upregulated fibronectin. Additionally, LOXL2 and LOXL2-caused matrix stiffening also increased the number of adherent HCC cells evidently, as well as the expression of chemokine CXCL2. Clinical data analysis demonstrated that HCC patients in High-LOXL2 group had higher ratio of microvascular invasion (OR=10.563, P=0.005) and tumor recurrence (OR=8.556, P=0.013) than HCC patients in Low-LOXL2 group, also revealing a significance of LOXL2 in HCC progression and unfavorable outcomeConclusion: Primary tumor-released LOXL2 contributes to the formation of lung pre-metastatic niche and the occurrence of lung metastasis. LOXL2 and LOXL2-caused matrix stiffening work together to induce pre-metastatic niche formation through influencing matrix remodeling and cell colonization. This study sheds light on the pathological significance of LOXL2 in lung pre-metastatic niche formation in HCC, also implicates a new intervention approach for HCC metastasis through reversal of lung pre-metastatic niche.
Employment transitions for spouses of stroke survivors: Evidence from Swedish national registries
Authors: Persson Josefine , Hensing Gunnel 
Published: May 2020

Abstract
Background: The sudden occurrence of stroke often leads to impaired physical, emotional, and cognitive abilities. Many stroke survivors therefore require support from their family members. However, little is known about the effects of a stroke event on the spouses’ employment transition probabilities. Thus, the aim of this study was to investigate whether a first ever stroke has an effect on employment transition probabilities for employed and unemployed spouses. The aim was also to analyze whether heterogeneity with respect to age, gender, education and comorbidities influence the size of the effect. Method: Data for this population-based cohort study were extracted from Swedish national registries from 2005 to 2016. The national sample consisted of 1,818 spouses of first ever stroke survivors during 2010 and 2011, and 7,399 matched controls that were employed or unemployed during five years prior stroke onset. Effects of stroke on spousal employment transitions were analyzed using linear regression, stratified by employment status prior to stroke onset.Results: Employed spouses prior stroke onset reduced their employment by -1.3 percentage points (95% CI: -2.4, -0.2). The data also indicated that employed spouses with lower age, comorbid conditions, and low educational attainment may be at even greater risk of transitioning to unemployment. On the other hand, stroke events appear to have limited impact on spouses that were unemployed prior to stroke onset. Conclusion: The risk of transitioning to unemployment appears to increase after stroke onset for spouses of stroke survivors, and disadvantaged groups may be at even greater risk. Thus, it is important for policy-makers to implement interventions to ensure that these groups of spouses have the possibilities to combine their caregiving role and remaining in the labor market.
Self-Medication Practice and Associated Factors Among Teachers’ Education Training College Students in Amhara Region, Ethiopia: A Cross-Sectional Study
Authors: Mekuria Abebe Basazn , Birru Eshetie Melese , Tesfa Melkamu Teshome , Geta Mestayet , Bykeda Tsegaw Amare 
Published: May 2020

Abstract
Background Self-medication practice is the use of medications without health care professional request. It leads to irrational medication usage, wastage of resources, increased chance of microbial resistance and adverse drug reaction. Therefore, this study aimed at assessing the prevalence associated factors of self-medication practice among students teacher’s education training college students in Amhara region, Ethiopia.Methods A multicentre cross-sectional study was conducted on 344 teacher's education training college students in Amhara region, from January 1 to February 28, 2020. Systematic random sampling was used to select participants and data collected through structured questionnaire. Descriptive statistics, univariate and multivariate logistic regression analysis were done to determine various variables and factors associated with self-medication practice.Results Out of 344 respondents, 68.0% were practiced self- medication. The most commonly cited indication for self-medication practice was headache 75 (32.05%) followed by abdominal discomfort 53 (22.6%). The respondents with age of greater than 26 years (AOR: 2.47, 95% CI: 1.18–3.94), third years of study (AOR: 3.14, 95% CI: 1.94–5.79), urban residence (AOR: 2.97, 95% CI: 1.06–3.64), near accessibility of pharmacy (AOR: 2.12, 95% CI: 1.43–4.46) and who had peer/family pressure (AOR: 2.34, 95% Cl: 1.53–3.56) were significantly associated with self-medication practice.Conclusion More than two-thirds of the study participants practiced self-medication. Being from the urban area, having access to private pharmacy and increase year of study are positively affects the self-medication practice.
The Modules of Mental Health Programs Implemented in Schools in Low- and Middle-Income Countries: Findings from a Systematic Literature Review
Authors: Gimba Solomon Musa , Harris Paul , Saito Amornrat , Udah Hyacinth , Martin Averil , Wheeler Amanda J. 
Published: May 2020

Abstract
Background Secondary schools in low- and middle-income countries (LMICs) provide promotional, preventive, and early treatment services. But the literature indicates that the modules of these services are either adapted or modified from existing mental health programs in developed countries. The literature also highlights the provision of non-comprehensive services (mental health promotion, prevention, and early treatment), in LMIC’s such as Nigeria. These findings inform the need for undertaking this systematic literature review. The aim of the review was to identify modules of school based mental health programs that have been implemented in LMICs to guide the development of a culturally sensitive comprehensive mental health program for adolescents in a LMIC.Methods The Preferred Reporting Items for Systematic reviews and Meta-analyses (PRISMA) statement was used to guide this review. The following databases were searched in September 2018, to identify the relevant literature: PubMed, CINAHL, Scopus, Web of Science, PsycINFO, and ERIC. The search was conducted by one of the authors and reviewed by two of the authors.Results Following the screening process, a total of 11 papers were identified and reviewed for quality. The systematic review highlighted that the mental health programs provided in schools included: introduction module, communication and relationship module, psychoeducation module, cognitive skills module, behavioral skills module, establishing social networks for recovery and help seeking behavioral activities and a summary/conclusion module.Conclusion The review shed light on the characteristics of the programs in LMIC. Two programs were universal in nature. Five programs were directed at key risk factors or at-risk groups and four were early intervention programs. The review also revealed that only one programs out of the 11 programs, included modules for parents. The synthesis indicated that all the identified programs were adapted or modified from existing programs. The dearth of comprehensive programs in LMIC was also revealed. The review also revealed modules that can be useful for developing a school based mental health program.
Maternal Stress, Depressive Symptoms and Body Mass Index of Adolescents: A Prospective Study
Authors: Koning Maaike , Vink Jacqueline , Visscher Tommy L.S. , Larsen Junilla 
Published: May 2020

Abstract
Background Changes in adolescent dietary and sedentary behaviors contribute to adolescence being one of the most vulnerable periods for the development of overweight and overweight-related morbidity. Children become more autonomous during adolescence, particularly in regard to decision-making over their own health behaviors. Despite this expanding autonomy, parents still play a major role in shaping health behaviors of adolescents and can still have influence on children’s weight outcomes. There is rising evidence that maternal stress and depressive symptoms are associated with young children’s weight outcomes. Longitudinal studies that test whether maternal stress or depressive symptoms may precede the development of adolescents’ weight outcomes are rare. This study aimed to fill this gap.Methods In this longitudinal cohort study data from 336 mothers and adolescents aged 10–14 years was used. Adolescents height and weight were measured, and both parents and adolescents filled in questionnaires on perceived stress and depressive symptoms. Regression analyses were performed in R to examine longitudinal links between stress and depressive symptoms at baseline (T1) and adolescents’ zBMI 6 months later (T2).Results Maternal general perceived stress (β = .14, p = .01) at T1 preceded the development of higher adolescents’ zBMI at T2, after controlling for baseline zBMI and other covariates, whereas maternal depressive symptoms at T1 (β = .04, p = .40) and other domain specific stress did not (maternal financial stress, maternal stress at work, maternal stress at home). Additionally, lower educational level among adolescents was associated with a higher zBMI at T2 (β = .16, p = .001).Conclusions Results suggest that maternal general stress, but not depressive symptoms, may influence adolescents’ weight development. Our findings warrant future investigation on whether and how general stress among mothers, but also fathers, may predict weight increases of their adolescent offspring.