bluuebunny/crossref_metadata_2025_split huggingface dataset:
Epidemiological, clinical, and virological characteristics of 465 hospitalized cases of coronavirus disease 2019 (COVID‐19) from Zhejiang province in China
Authors: Lian Jiangshan , Jin Xi , Hao Shaorui , Jia Hongyu , Cai Huan , Zhang Xiaoli , Hu Jianhua , Zheng Lin , Wang Xiaoyan , Zhang Shanyan , Ye Chanyuan , Jin Ciliang , Yu Guodong , Gu Jueqing , Lu Yingfeng , Yu Xiaopeng , Xiang Dairong , Li Lanjuan , Liang Tingbo , Sheng Jifang , Yang Yida 
Published: May 2020

AbstractBackgroundThe severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) and the associated coronavirus disease (COVID‐19) have spread throughout China. Previous studies predominantly focused on its place of origin, Wuhan, causing over estimation of the disease severity due to selection bias. We analyzed 465 confirmed cases in Zhejiang province to determine the epidemiological, clinical, and virological characteristics of COVID‐19.MethodsEpidemiological, demographic, clinical, laboratory, and management data from qRT‐PCR confirmed COVID‐19 patients from January 17, 2020, to January 31, 2020, were collected, followed by multivariate logistic regression analysis for independent predictors of severe/critical‐type COVID‐19 and bioinformatic analysis for features of SARS‐CoV‐2 from Zhejiang province.ResultsAmong 465 COVID‐19 patients, median age was 45 years, while hypertension, diabetes, and chronic liver disease were the most common comorbidities. History of exposure to the epidemic area was present in 170 (36.56%) and 185 (39.78%) patients were clustered in 77 families. Severe/critical‐type of COVID‐19 developed in 49 (10.54%) patients. Fever and cough were the most common symptoms, while diarrhea/vomiting was reported in 58 (12.47%) patients. Multivariate analysis revealed eight risk factors for severe/critical COVID‐19. Glucocorticoids and antibiotics were administered to 60 (12.90%) and 218(46.88%) patients, respectively. Bioinformatics showed four single amino acid mutations and one amino acid position loss in SARS‐CoV‐2 from Zhejiang province, with more similarity to humans than to viruses.ConclusionsSARS‐CoV‐2 showed virological mutations and more human transmission in Zhejiang province, indicating considerable epidemiological and clinical changes. Caution in glucocorticoid and antibiotics use is advisable.
Molecular mechanism of c‐Myc and PRPS1/2 against thiopurine resistance in Burkitt's lymphoma
Authors: Li Ting , Song Lili , Zhang Yingwen , Han Yali , Zhan Zhiyan , Xv Zhou , Li Yang , Tang Yuejia , Yang Yi , Wang Siqi , Li Shanshan , Zheng Liang , Li Yanxin , Gao Yijin 
Published: May 2020

AbstractPatients with relapsed/refractory Burkitt's lymphoma (BL) have a dismal prognosis. Current research efforts aim to increase cure rates by identifying high‐risk patients in need of more intensive or novel therapy. The 8q24 chromosomal translocation of the c‐Myc gene, a main molecular marker of BL, is related to the metabolism by regulating phosphoribosyl pyrophosphate synthetase 2 (PRPS2). In our study, BL showed significant resistance to thiopurines. PRPS2 homologous isoenzyme, PRPS1, was demonstrated to play the main role in thiopurine resistance. c‐Myc did not have direct effects on thiopurine resistance in BL for only driving PRPS2. PRPS1 wild type (WT) showed different resistance to 6‐mercaptopurine (6‐mp) in different metabolic cells because it could be inhibited by adenosine diphosphate or guanosine diphosphate negative feedback. PRPS1 A190T mutant could dramatically increase thiopurine resistance in BL. The interim analysis of the Treatment Regimen for Children or Adolescent with mature B cell non‐Hodgkin's lymphoma in China (CCCG‐B‐NHL‐2015 study) confirms the value of high‐dose methotrexate (MTX) and cytarabine (ARA‐C) in high‐risk paediatric patients with BL. However, there remains a subgroup of patients with lactate dehydrogenase higher than four times of the normal value (4N) for whom novel treatments are needed. Notably, we found that the combination of thiopurines and the phosphoribosylglycinamide formyltransferase (GART) inhibitor lometrexol could serve as a therapeutic strategy to overcome thiopurine resistance in BL.
Effects of inbreeding on behavioural plasticity of parent–offspring interactions in a burying beetle
Authors: Ratz Tom , Perodaskalaki Anastasia , Moorad Jacob , Smiseth Per T. 
Published: May 2020

AbstractInbreeding depression is defined as a fitness decline in progeny resulting from mating between related individuals, the severity of which may vary across environmental conditions. Such inbreeding‐by‐environment interactions might reflect that inbred individuals have a lower capacity for adjusting their phenotype to match different environmental conditions better, as shown in prior studies on developmental plasticity. Behavioural plasticity is more flexible than developmental plasticity because it is reversible and relatively quick, but little is known about its sensitivity to inbreeding. Here, we investigate effects of inbreeding on behavioural plasticity in the context of parent–offspring interactions in the burying beetle Nicrophorus vespilloides. Larvae increase begging with the level of hunger, and parents increase their level of care when brood sizes increase. Here, we find that inbreeding increased behavioural plasticity in larvae: inbred larvae reduced their time spent associating with a parent in response to the length of food deprivation more than outbred larvae. However, inbreeding had no effect on the behavioural plasticity of offspring begging or any parental behaviour. Overall, our results show that inbreeding can increase behavioural plasticity. We suggest that inbreeding‐by‐environment interactions might arise when inbreeding is associated with too little or too much plasticity in response to changing environmental conditions.
Individual and contextual predictors of retention in Special Olympics for youth with intellectual disability: who stays involved?
Authors: Weiss J. A. , Robinson S. , Harlow M. , Mosher A. , Fraser‐Thomas J. , Balogh R. , Lunsky Y. 
Published: May 2020

AbstractBackgroundDespite the recognised benefits of sport, participation is often reported to be low for youth with intellectual disability (ID). The current study was the first to longitudinally examine sport retention in this population, a critical aspect of ensuring participation.MethodsStudy participants were parents/caregivers of athletes with ID involved in community Special Olympics (SO), 11–22 years of age (N = 345). Participants completed an online survey in 2012 that included caregiver demographic and athlete intrapersonal, interpersonal and broader contextual variables. Retention rates for 2019 were determined using the SO provincial registration lists.ResultsOf the 345 survey participants, 81.7% remained active athletes in 2019. Caregiver demographic and athlete intrapersonal factors were largely unrelated to retention. In contrast, retention was associated with the frequency and number of sports athletes participated in, the perceived psychosocial gains of SO involvement and the environmental supports that were available to facilitate participation; frequency of sport participation was the strongest predictor of remaining a registered athlete.ConclusionsThis study has implications for future initiatives aimed at increasing sport retention in a population that struggles to be engaged in sport. Efforts should focus on the athlete experience and sport‐specific factors. Coaches and caregivers can foster positive experiences and play an important role in continued sport participation.
MR Intracranial Vessel Wall Imaging: A Systematic Review
Authors: Song Jae W. , Moon Brianna F. , Burke Morgan P. , Kamesh Iyer Srikant , Elliott Mark A. , Shou Haochang , Messé Steven R. , Kasner Scott E. , Loevner Laurie A. , Schnall Mitchell D. , Kirsch John E. , Witschey Walter R. , Fan Zhaoyang 
Published: May 2020

ABSTRACTThe purpose of this systematic review is to identify trends and extent of variability in intracranial vessel wall MR imaging (VWI) techniques and protocols. Although variability in selection of protocol design and pulse sequence type is known, data on what and how protocols vary are unknown. Three databases were searched to identify publications using intracranial VWI. Publications were screened by predetermined inclusion/exclusion criteria. Technical development publications were scored for completeness of reporting using a modified Nature Reporting Summary Guideline to assess reproducibility. From 2,431 articles, 122 met the inclusion criteria. Trends over the last 23 years (1995‐2018) show increased use of 3‐Tesla MR (P < .001) and 3D volumetric T1‐weighted acquisitions (P < .001). Most (65%) clinical VWI publications report achieving a noninterpolated in‐plane spatial resolution of ≤.55 mm. In the last decade, an increasing number of technical development (n = 20) and 7 Tesla (n = 12) publications have been published, focused on pulse sequence development, improving cerebrospinal fluid suppression, scan efficiency, and imaging ex vivo specimen for histologic validation. Mean Reporting Summary Score for the technical development publications was high (.87, range: .63‐1.0) indicating strong scientific technical reproducibility. Innovative work continues to emerge to address implementation challenges. Gradual adoption into the research and scientific community was suggested by a shift in the name in the literature from “high‐resolution MR” to “vessel wall imaging,” specifying diagnostic intent. Insight into current practices and identifying the extent of technical variability in the literature will help to direct future clinical and technical efforts to address needs for implementation.
Metformin inhibits extracellular matrix accumulation, inflammation and proliferation of mesangial cells in diabetic nephropathy by regulating H19/miR-143-3p/TGF-β1 axis
Authors: Xu Jiang , Xiang Ping , Liu Linqing , Sun Jianran , Ye Shandong 
Published: May 2020

Abstract

Objectives
Metformin (MET) has protective effect on diabetic nephropathy (DN). This study aims to demystify the mechanism of MET function in DN.

Methods
Mouse glomerular membrane epithelial cell line SV40-MES-13 was treated with normal or high glucose combined with or without MET. The relationships among H19, miR-143-3p and TGF-β1 were evaluated by luciferase reporter assay. MTT assay was performed to detect cell proliferation. The levels of inflammatory factors were investigated by enzyme-linked immunosorbent assay. Quantitative real-time PCR and western blot were performed to examine gene and protein expression.

Key Findings
H19 was up-regulated in the SV40-MES-13 cells after treated with high glucose, which was effectively repressed by MET treatment. MET promoted extracellular matrix accumulation, inflammation and proliferation in the SV40-MES-13 cells after treated with high glucose. These influences conferred by MET were abolished by H19 overexpression. H19 regulated TGF-β1 expression by sponging miR-143-3p. Furthermore, MET inhibited extracellular matrix accumulation, inflammation and proliferation by regulating H19/miR-143-3p/TGF-β1 axis.

Conclusions
Our studies demonstrated that the protective effect of MET on DN was attributed to the inhibition of proliferation, inflammation and ECM accumulation in mesangial cells via H19/miR-143-3p/TGF-β1 axis, which suggested that the H19/miR-143-3p/TGF-β1 axis could be a valuable target for DN therapies.
Association between morningness‐eveningness, sleep duration, weekend catch‐up sleep and depression among Korean high‐school students
Authors: Koo Dae Lim , Yang Kwang Ik , Kim Jee Hyun , Kim Daeyoung , Sunwoo Jun‐Sang , Hwangbo Young , Lee Hwa Reung , Hong Seung Bong 
Published: May 2020

AbstractThe present study aimed to examine the association between morningness‐eveningness preferences, sleep duration, weekend catch‐up sleep duration and depression among Korean high‐school students. A total of 8,655 high‐school students participated from 15 districts in South Korea and completed an online self‐report questionnaire. The following sleep characteristics were assessed: weekday and weekend sleep duration, weekend catch‐up sleep duration, morningness‐eveningness preference, perceived sufficiency of sleep, self‐reported snoring and sleep apnea, daytime sleepiness, and sleep environment. Age, gender, body mass index, number of private classes, proneness to internet addiction, and depressive mood were also evaluated. A logistic regression analysis was conducted to compute odds ratios for the association between depression and sleep characteristics, after controlling for relevant covariates. Eveningness preference was a significant predictor of depressive mood (adjusted OR, 1.71; 95% CI, 1.47–1.99). Weekend CUS durations that were ≥2 hr and enrollment in numerous private classes were associated with a lower risk for depression (0.68, 0.55–0.85; 0.76, 0.60–0.95; respectively). Female gender, underweight and obese body weight, short weekday sleep durations, excessive daytime sleepiness, perceived excessiveness and insufficiency of sleep, self‐reported snoring and sleep apnea, proneness to internet addiction and a non‐optimal sleep environment were associated with an increased risk for depression. Eveningness preference and insufficient weekday sleep duration were associated with an increased risk for depression. Weekend CUS duration ≥2 hr reduced the risk for depression. Diverse aspects, including sleeping habits and sleep‐related environmental factors, should be considered to reduce depressive symptoms in late adolescents.
Differential expression of akirin gene in black tiger shrimp Penaeus monodon in response to immunostimulant administration and infections with Vibrio harveyi and white spot syndrome virus
Authors: Dechamma Mundanda M. , Mani Madhu K. , Rajeish Moger , Santhosh Kogaluru S. , Akhila Dharnappa S. , Maiti Biswajit , Bossier Peter , Karunasagar Iddya , Karunasagar Indrani 
Published: May 2020

AbstractThe akirin gene, which is strictly localized in the nucleus, plays a critical role in regulating antimicrobial peptide transcription, and has parallel functions to NF‐κB signaling pathway in both vertebrates and invertebrates. In shrimp, the akirin gene is expressed as innate immunity in response to microbial infection. In the present study, expression of akirin gene in Penaeus monodon with respect to Vibrio harveyi and white spot syndrome virus (WSSV) infections and immunostimulant (β‐glucan) administration were investigated by quantitative polymerase chain reaction. The gene was expressed in various tissue samples of healthy shrimp. Maximum level of expression was immediately after V. harveyi infection, suggesting that it may be an early response gene. Gene expression was remarkably upregulated in the lymphoid organ, gill, and hepatopancreas, whereas downregulation was observed in hemocytes compared with the control. In the case of WSSV‐infected samples, the akirin gene was significantly downregulated in the lymphoid organ but there was no significant difference in expression pattern in hemocytes compared to the control. In gill tissue, maximum expression was observed after 2 hr of infection, the same in hepatopancreas. Experimental challenge of β‐glucan fed shrimp infected with V. harveyi and WSSV resulted in significant upregulation of akirin gene expression in lymphoid and gill tissue.
The Role of Linguistic Alignment on Question Development in Face‐to‐Face and Synchronous Computer‐Mediated Communication Contexts: A Conceptual Replication Study
Authors: Kim YouJin , Skalicky Stephen , Jung YeonJoo 
Published: May 2020

AbstractTo date, linguistic alignment studies in second language acquisition have mainly been conducted during face‐to‐face (FTF) interactions. In the current study, we examined and compared the effect of structural alignment on the development of English direct and indirect questions in FTF and synchronous computer‐mediated communication (SCMC) contexts. Additionally, we assessed participants’ working memory capacity and previous knowledge of the target structures. We assigned 50 Korean learners of English to either an FTF or SCMC modality. Over 3 weeks, they completed a pretest, two interactive alignment sessions, a working memory test, and immediate and delayed posttests. Results indicate that primed production of direct and indirect questions is significantly more likely than unprimed production. Moreover, we found an interaction with modality for primed production of direct questions but not for indirect questions. Findings suggest learning effects for direct questions only and that this effect is facilitated by the degree of alignment.
Global patterns in seed germination after ingestion by mammals
Authors: Torres Diego A. , Castaño John H. , Carranza‐Quiceno Jaime A. 
Published: May 2020

Abstract

Mammals play an important role in seed germination through the ingestion of fruits and seeds. Since seed germination is a basic step in seedling recruitment, understanding how mammals affect germination improves our understanding of the effect of loss of mammal populations on the dynamics of plant communities.

We used meta‐analytical methods to describe global patterns in the effect of seed ingestion by mammals on seed germination success and rate. We collected data from 154 studies that included 115 mammal species and 448 plant species.

Our results showed a positive cumulative effect of mammals on seed germination. However, this effect differed between mammalian orders; thus, some groups such as elephants, primates, and new world marsupials emerged as important enhancers of seed germination. Also, the effect varied depending on the plant family and the bioregion. Increased seed germination after ingestion was positively related to fast germination.

This meta‐analysis, the first to synthesise and compare most of the information presently available on how mammals affect seed germination after ingestion, shows a global positive effect of mammals as enhancers of seed germination. However, behind that positive effect lies a diversity of neutral, negative, and positive effects of different magnitudes, which may have multifactorial explanations. We hope that the patterns presented here open up new questions and help guide future research efforts.
Physical activity is associated with accelerated gastric emptying and increased ghrelin in obesity
Authors: Davis Judith , Camilleri Michael , Eckert Deborah , Burton Duane , Joyner Michael , Acosta Andres 
Published: May 2020

AbstractBackgroundRapid gastric emptying, increased food intake, and alterations in gastrointestinal hormones are associated with obesity. The effect of regular physical activity (PA) on food intake, gastric emptying (GE), gastric accommodation, and gastrointestinal (GI) hormones in adults with obesity remains unclear. Our aim was to compare, at time of presentation, weight trends, eating behavior, GE, and GI hormone levels among individuals with obesity who engage in regular PA compared to those who do not.MethodsIn 270 participants with obesity, we performed validated measurements of GI phenotypes: GE of solids and liquids, gastric volume (GV) during fasting and after consumption of 200 mL Ensure®, satiety by kcal intake (T‐kcal) during a buffet meal, satiation (volume to fullness [VTF] and maximal tolerated volume [MTV]) of a liquid nutrient, and plasma levels of fasting and postprandial GLP‐1, PYY, CCK, and ghrelin. Physical Activity Stages of Change Questionnaire was used to assess whether participants were regularly PA or not.Key resultsPA was associated with lower BMI (Δ 2.01 kg/m2, P = .001) and body weight (Δ 4.42 kg, P = .0278). GE of solids (T‐50% Δ 7.54 min, P = .021) and liquids (T‐50% Δ 2.99 min, P = .029%) was significantly more rapid in physically active participants. PA was also associated with relatively higher postprandial ghrelin AUC (Δ 10.4 pg/mL, P = .015). There was no significant difference in postprandial satiation, satiety, GV, or other GI hormones (CCK, PYY, or GLP‐1) between groups.Conclusions & InferencesPhysical activity is associated with lower BMI, but faster GE and higher postprandial ghrelin levels, two factors that are also associated with obesity.
Shoot‐level flammability across the Dracophyllum (Ericaceae) phylogeny: evidence for flammability being an emergent property in a land with little fire
Authors: Cui Xinglei , Paterson Adrian M. , Alam Md Azharul , Wyse Sarah V. , Marshall Kate , Perry George L. W. , Curran Timothy J. 
Published: May 2020

Summary

Plant flammability varies across species, but the evolutionary basis for this variation is not well understood. Phylogenetic analysis of interspecific variation in flammability can provide insights into the evolution of plant flammability.

We measured four components of flammability (ignitability, sustainability, combustibility and consumability) to assess the shoot‐level flammability of 21 species of Dracophyllum (Ericaceae). Using a macroevolutionary approach, we explored phylogenetic patterns of variation in shoot‐level flammability.

Shoot‐level flammability varied widely in Dracophyllum. Species in the subgenus Oreothamnus had higher flammability and smaller leaves than those in the subgenus Dracophyllum. Shoot flammability (ignitability, combustibility and consumability) and leaf length showed phylogenetic conservatism across genus Dracophyllum, but exhibited lability among some closely related species, such as D. menziesii and D. fiordense. Shoot flammability of Dracophyllum species was negatively correlated with leaf length and shoot moisture content, but had no relationship with the geographic distribution of Dracophyllum species.

Shoot‐level flammability varied widely in the genus Dracophyllum, but showed phylogenetic conservatism. The higher flammability of the subgenus Oreothamnus may be an incidental or emergent property as a result of the evolution of flammability‐related traits, such as smaller leaves, which were selected for other functions and incidentally changed flammability.
Photobiomodulation therapy inhibits oral submucous fibrosis in mice
Authors: Chiang Min‐Hsuan , Lee Kun‐Tsung , Chen Chia‐Hsin , Chen Ker‐Kong , Wang Yan‐Hsiung 
Published: May 2020

AbstractObjectivesOral submucous fibrosis (OSMF) is a chronic inflammatory disease and a potentially malignant oral disorder. However, the best therapeutic treatment for OSMF remains uncertain. Our previous study showed that photobiomodulation (PBM) therapy and forskolin could reduce arecoline‐induced fibrosis reactions via the cAMP pathway. The present study aimed to establish an animal model of areca nut extract (ANE)‐induced OSMF and to evaluate the therapeutic potential of PBM and forskolin for ANE‐induced OSMF.Subjects and methodsThe mice were divided into five groups. The buccal tissues were harvested for histomorphological analysis and immunoblotting.ResultsOur results showed that PBM significantly reduced the development of ANE‐induced OSMF, quantified by changes in submucosal layer thickness and collagen deposition. Additionally, PBM could extensively reduce the protein expression of the fibrotic marker genes alpha‐smooth muscle actin (α‐SMA) and connective tissue growth factor (CTGF) in buccal submucous lesions. However, forskolin treatment significantly decreased the protein expression of fibrotic marker genes but slightly decreased the observed histomorphological changes.ConclusionsWe established an ANE‐induced OSMF mouse model, which also provided a model for the development of a therapeutic treatment for OSMF. The anti‐fibrotic effects of PBM and forskolin may be useful for clinical interventions.