bluuebunny/crossref_metadata_2025_split huggingface dataset:
Portable Continuous Wave Doppler Ultrasound for Primary Healthcare in South Africa: Can the EUnetHTA Core Model Guide Evaluation Before Technology Adoption?
Authors: Mueller Debjani , Pattinson Robert C , Hlongwane Tsakane Musa , Busse Reinhard , Panteli Dimitra 
Published: September 2020

Abstract
Background: This study had a threefold aim: to test the value of stakeholder involvement in HTA to reduce the evidence gaps and interpret findings; applicability of the EUnetHTA Core Model (CM) in South Africa on a medical device and thus ultimately provide a first overview of evidence for potential widespread adoption of the technology in a primary health care setting. Used in primary healthcare setting for obstetric use, the technology under assessment is a low-cost continuous wave Doppler ultrasound.Methods: To substantiate the evidence obtained from the literature different stakeholders were identified and consulted. The scoping of the assessment was defined by involving the policy makers to select the domains and the analogous questions relevant to the ultrasound and its use. Additionally, hospital managers were invited to respond to dichotomous questions on the criteria for procurement. The evidence generated from the four steps was used to populate the high-ranked assessment elements of the CM. Results: The HTA took into account the clinical benefit in screening low-risk pregnant women in primary healthcare settings using the technology. It incorporated the evidence on organizational, ethical, and social value of its use together with effectiveness, safety, and cost-effectiveness of the technology. The domains on “health problem” and “safety” had a higher rank than the rest of the nine domains. Conclusions: A multi-stakeholder participation in the assessment process provided the necessary transparency and accountability, thus improving the quality of the recommendation resulting from the assessment. The collection of robust evidence and analysis post-introduction will enable the determination of the value of the technology in real-world settings.
Emodin induced necroptosis and inhibited glycolysis in the renal cancer cells by enhancing ROS
Authors: Wang Ke-jie , Meng Xiang-yu , Chen Jun-feng , Wang Kai-yun , Zhou Cheng , Yu Rui , Ma Qi 
Published: September 2020

Abstract
Background: Renal cell carcinoma (RCC) is a tumor with unpredictable presentation and poor clinical outcome. RCC is always resistant to chemotherapy, radiation, and weakly sensitive to immunotherapeutic agents. Therefore, novel agents and approaches are urgently needed for the treatment of RCC. Emodin, an anthraquinone compound extracted from rhubarb and other traditional Chinese herbs, has been implicated in a wide variety of pharmacological effects, such as anti-inflammatory, antiviral, and antitumor activities. However，its role in RCC remains unkown. Methods: Flow cytometry assay and lactate dehydrogenase release assay were used to detect the cell death. Reactive oxygen species was tested by the dye MitoSox and DCFH-DA. Glucose-6-phosphate, pyruvate and ATP level were measured to evaluate the glycolysis process. Western blot was used to detect protein expression. Results: Emodin effectively killed renal cancer cells without significant toxicity to normal renal tubular epithelial cell. Flow cytometry assay with Annexin V-FITC and PI demonstrated that emodin induces necroptosis, but not apoptosis, in renal cancer cells. Meanwhile, the phosphorylation levels of RIP1 and MLKL, the key necroptosis-related proteins, were significantly increased. To explore how emodin inhibits kidney tumor growth, reactive oxygen species (ROS) levels were tested and the levels of ROS increased upon emodin treatment in a dose-dependent manner. Further studies demonstrated that emodin induced necroptosis through ROS-mediated activation of JNK signaling pathway, and also inhibited glycolysis by down-regulation GLUT1 by ROS-mediated inactivation of PI3K/AKT signaling pathway. Conclusion: These findings revealed the potential mechanisms by which emodin suppresses renal cancer cell growth, and will help develop novel therapeutic approaches for patients with JNK- or PI3K/AKT-dysregulated renal cancer.
A Fast CU Size Decision Optimal Algorithm based on Coding Bits for HEVC
Authors: Wang Jianhua , Chen Zhihao , Lin Feng , Zhao Jing , Long Yongbing , Lan Yubin 
Published: September 2020

Abstract
HEVC (High Efficiency Video Codng) employs quadtree CTU (Coding Tree Unit) structure to improve its coding efficiency, but at the same time, it also requires a very high computational complexity due to its exhaustive search process for an optimal partition mode for the current CU(Coding Unit). Aiming to solve the problem, a fast CU size decision optimal algorithm based on coding bits is presented for HEVC in this paper. The contribution of this paper lies that we successfully use the coding bits technology to quickly determine the optimal partition mode for the current CU. Specially, in our scheme, firstly we carefully observe and statistically analyze the relationship among the texture complexity and partition size and coding bits in the CUs of video image; Secondly we find the correlation between coding bits and partition size based on the relationship above; Thirdly, we build the corresponding threshold of coding bits for partition size under different CU size and QP value based on the correlation above to reduce many unnecessary prediction and partition operations for the current CU. As a result, our proposed algorithm can effectively saving lots of computational complexity for HEVC. The simulation results show that our proposed fast CU size decision algorithm based on coding bits in this paper can save about 34.67% coding time, and only at a cost of 0.61% BDBR increase and 0.043db BDPSNR decline compared with the standard reference of HM16.1, thus improving the coding performance of HEVC.
Great Obstetrical Syndromes In G20210a Mutation Carriers Associated With High Prothrombin Activity
Authors: Nikolaeva Mariya , Momot Andrey Pavlovich , Zainulina Marina Sabirovna , Yasafova Natalia Nikolaevna , Taranenko Irina Alekseevna 
Published: September 2020

Abstract
Objective: to study the association between high activity of Factor II (prothrombin) in blood plasma with G20210A mutation and the development of great obstetrical syndromes.Material and methods: A prospective clinical cohort study was conducted on 290 pregnant women (average age 31.7±4.7 years old). The main group was made up of 140 G20210A patients, while the control group comprised 150 women with the wild G20210G type. The aim was to evaluate the activity of Factor II in the venous blood plasma during the stages of pregnancy with regard to trophoblast invasion waves. As per results, association analysis of Factor II activity value and gestational complications was carried out.Results: In the control group, the median (Me) of Factor II activity ranged from 108% (preconception period) to 144% (pregnancy) [95% CI 130-150]. In patients with the GA type, the value was significantly higher in related periods, ranging from 149% to 181% [95% CI 142-195], p<0.0001. With Factor II activity ranging from 148.5% to 180.6%, pregnancies in the main group had no complications. Higher levels of Factor II activity were associated with the development of early and/or severe preeclampsia (PE) and fetal growth retardation (FGR).Conclusion: The data obtained regarding Factor II activity in blood plasma, juxtaposed with the development of great obstetrical syndromes, allow to assume that manifestation of G20210A in early and/or severe PE and FGR is associated with this coagulation factor's level of activity. Threshold value of the Factor II activity with G20210A mutation, allowing to predict the development of PE, comprised 171.0% at the preconception stage (AUC – 0.86; p<0.0001) and within 7-8 weeks of gestation it was 181.3% (AUC – 0.84; p<0.0001).
Disability Related to Function of the Upper Extremities in Early Rheumatoid Arthritis – Course and Relation to Other Disease Parameters Over 10 Years: A Cohort Study
Authors: RYDHOLM MARIA , WIKSTROM INGEGERD , HAGEL SOFIA , JACOBSSON LENNART T.H. , TURESSON CARL 
Published: September 2020

Abstract
Background: The objective of this study was to investigate the course of disability related to the upper extremities (UE) in early rheumatoid arthritis (RA), and to assess correlations between such disability and clinical parameters, including grip force. Methods: In an inception cohort of patients with early RA (N=222), disability of the UE was assessed using a subscore of the Health assessment questionnaire disability index (HAQ-DI), and average grip force of the dominant hand was measured. Changes between consecutive follow-up visits in the HAQ-DI-UE subscore were assessed using the paired samples t-test, and correlations with key disease parameters using Spearman’s rank test. The relation between joint involvement and HAQ-DI-UE was examined using multivariate linear regression analysis. Results: The HAQ-DI-UE decreased significantly from inclusion to the 6-month follow-up (mean change -0.26; 95% CI -0.18 to -0.34), and increased significantly after 2 years. There were fairly strong correlations for HAQ-DI-UE with grip force (r: -0.50 to -0.62), patient’s global assessment (r:0.58 to 0.64) and patient’s assessment of pain (r:0.54 to 0.60) at all time points up to 5 years, but only moderate to weak correlations with swollen joints, CRP and ESR. At inclusion wrist synovitis and tender PIP joints had both an independent impact on HAQ-DI-UE, whereas tenderness of the shoulder and the wrist had a greater importance at 6 months. Conclusions: Disability related to the upper extremities decreased significantly during the first 6 months, and increased again after 2 years. The correlations with clinical parameters underline the major impact of pain and impaired hand function in early RA.
Hydrolyzed FB1 (aminopentol) detection on urine of HPV positive Mexican women: A preliminary study
Authors: Ramírez-Cisneros M. Ángeles , Rivera Vladimir , Rios Maria Yolanda , Vargas-De-León Cruz , Dávila Sonia , Peralta Raúl 
Published: September 2020

Abstract
Human Papillomavirus (HPV) infection is the most common sexually transmitted disease in humans. About 200 HPV types have been described and classified into high and low oncogenic risk for its ability to transform the cell. Some main risk factors for HPV infection are multiple sexual partners, multiparity and immunosuppression. However, these factors can not completely explain the HPV prevalence in different world regions, Mexico has a high global burden, being important to determine other external biological factors implicated. The infection is mediated by surface receptors of basal stratum cells that bind to the viral capsid entering the cell by endocytosis. Some cell membrane components such as sphingolipids have a role in structure and membrane permeability. Besides, fumonisins are micotoxins structurally very similar to sphingolipids, fumonisin B1 (FB1) is the most abundant and most similar to some sphingolipids such ceramide. Fumonisins are produced by Fusarium fungus, widely distributed in maize, the main component of Mexican diet. Thus, it is possible that FB1 contamination be a factor to permeabilize the membrane expediting the HPV infection. In this preliminary study, we postulated that mechanism of HPV infection is mediated by contamination with FB1, hence, FB1 could be higher in women HPV positive. Urine samples from patients with HPV infection and healthy woman were analyzed by UPLC-MS, hydrolyzed FB1 was found only in the infected women, in contrast, in HPV negative women was not detected. Additional studies are needed to support the hypothesis of contamination with FB1 as biological risk factor for HPV infection.
SG and SSR approach in the preparation of precursors and influence on superconducting properties of Tl-1223 superconductors
Authors: Metskhvarishvili Ioseb , LOBZHANIDZE T.E. , DGEBUADZE G.N. , BENDELIANI B.G. , METSKHVARISHVILI M.R. , RUSIA M.Sh. , GABUNIA V.M. , KOMAKHIDZE K. 
Published: September 2020

Abstract
The paper presents the comparative analysis of the Sol-gel (SG) and solid-state reaction (SSR) route for the synthesis of precursors for thallium-based superconductors. Samples were prepared a two-step method and by sealed quartz tube technique in ambient pressure. Heat treatments of precursors showed that to obtain high-purity precursors, without any carbonate contamination for SG methods is sufficient to heat treatments at 915oC temperatures and for SSR methods 945oC. The diamagnetic onset temperature of the superconducting transition for the TlBa2Ca2Cu3O8+δ samples prepared by precursors SG at 915oC and SSR at 945oC is about T = 120 K and full screening of applied ac magnetic fields observed at T ≈ 102 K and T ≈ 94 K, respectively. The value of Jc transport critical current densities for SSR obtained 128 A/cm2, whereas for the SG sample and exactly 174 A/cm2. We could conclude that the, using the wet chemistry offers some advantages in comparison with the classical solid-state ceramics processing, especially, better chemical homogeneity and higher reactivity of the precursor powder.
Embryos from "0PN" Zygotes May Be Most Likely 2PN Embryo in L-R-ICSI
Authors: Liu Caixia , Han Dong , Chen Jianguo , Liu Ying , Li Dong , Zhou Yan , Zhang Chaofan , Liu Jie 
Published: September 2020

Abstract
Although some zero pronuclei (0PN) zygotes cleave and develop into good embryos, they are usually discarded because they lack two distinct pronuclei (2PN). In this case report, we followed four couples to determine whether 0PN embryo could be used in late rescue intracytoplasmic sperm injection (L-R-ICSI). Here we report five healthy infants, including one set of twins, derived from "0PN" embryos from four frozen–warmed embryo transfer (FET) cycles in L-R-ICSI between 2015 and 2017. Of nine infants born from L-R-ICSI cycles in our center, five were "0PN" embryos, and all remain healthy. Embryos from "0PNs" such as 2PN can develop into healthy babies using L-R-ICSI. This finding suggests that embryos from "0PNs" in L-R-ICSI may be different from traditional 0PN gametes and more likely to originate from 2PN gametes and they may be used in those infertile couples who lack 2PN embryos, instead of discarding them. The use of "0PN" embryos increases the cycles reaching embryo transfer, allowing some infertile couples to have healthy children and to avoid mental anguish and wasted time and money.
Global Research on DNA methylation from 2000 to 2019:&nbsp;Bibliometric Analysis and Visualization
Authors: Su Xuan , Weng Lin-Man , Zheng Kang-Yong , Wang Xue-Qiang 
Published: September 2020

Abstract
Introduction:DNA methylation is the most common epigenetic feature in human somatic cells, it has played a crucial role in regulating related genes and diagnosing cancer. However, few studies used bibliometric method to systematically analyze the development of DNA methylation. The purpose of this study was to systematically analyze the trends of DNA methylation research from 2000 to 2019. Methods:The published studies were searched between January, 2000 and December 31st, 2019 in the Web of Science. We only included peer-reviewed reviews and articles. The language was limited to English, and no species limitations were specified. A total of 11,127 publications met the inclusion criteria. CiteSpace V was used to analyze the trends of DNA methylation research. Results:The results showed the publications had a statistical increase over time in the DNA methylation research(P<0.005) by linear regression analyses. USA had the largest number of published papers (4263). Plos One contributed to the most publications (4.52%). The most prolific institution is Chinese Acad Sci (237). In accordance with subject categories of Web of Science, Genetics Heredity subject category had the largest number of publications (2,516) and citations (119,113). Biochemistry Molecular Biology subject category had the highest number of H-index (154). On the basis of the co-citation map of references, the “specific dna methylation” was labeled as the largest cluster. Most recent burst keywords were as follows: “pregnancy” (2017~2019), “obesity” (2017~2019), “growth” (2017~2019). Conclusions: This study provides useful information for DNA methylation researchers to find fresh viewpoints related to collaborators, cooperative institutions, popular topics. It also reminds us to pay attention to some new research trends.
Specific pathogens indicate higher risk of polymicrobial periprosthetic joint infection: a single-center retrospective study
Authors: Li Hao , Jiang Qiao , Chen Jiying 
Published: September 2020

Abstract
Background

Polymicrobial periprosthetic joint infection is a subset of periprosthetic joint infection and indicates disastrous outcomes. However, the incidence of polymicrobial PJI can be underestimated so we perform a single-center retrospective study with an intention to predict the incidence of polymicrobial PJI based on culture results.
Materials and methods

Medical records about 153 patients with PJI between 2017/1/1 and 2018/12/31 in a joint center were selected retrospectively. Multivariable analysis was utilized to evaluate the association between various pathogens and polymicrobial PJI.
Result

Staphylococcus aureus (OR:3.83, 95%CI(1.30,11.27), p-value = 0.015 ), coagulase negative staphylococci (OR: 2.68 ,95%CI: (1.10, 6.25), p-value = 0.028), Enterococcus spp (OR:6.46;95%CI(1.35,30.87),P-value = 0.020)andStreptococcusspp(OR:19.38, 95%CI:(3.57,105.21), p-value = 0.001) indicate higher risk of polymicrobial pathogens compared with other pathogens
Conclusion

Streptococcus spp and Enterococcus spp are associated with higher risk of polymicrobial PJI and more pathogens are like to be found in additional culture when these pathogens are detected by a single culture.
A Modified Method to Determine the Humerus Height and Humeral Head Retroversion for Hemiarthroplasty
Authors: Wei Wanfu , Li Jian`an , Bu Guoyun , Yang Tao 
Published: September 2020

Abstract
Background Placement of shoulder prosthesis used for reconstruction of a complex proximal humerus fractures is critical because it is related to clinical outcomes after hemiarthroplasty. To achieve the anatomical placement of the prosthesis, we introduced a simple operative method to determine the humerus height and humeral head retroversion, and retrospectively investigated the clinical and radiographic outcomes of our procedure.Method 34 patients, treated by shoulder arthroplasty for 4-part or 3–part proximal humeral fracture during the period between June 2016 and December 2018, were enrolled in the study. Eventually 29 patients, 18 women and 11 men with an average age of 68.4 years (range 58-85 years), were followed up successfully. Of which 13 patients treated with classic method were matched to 19 of the 29 new method treated patients by operation time, blood loss, pain, range of motion, Constant-Murley score and radiographic features. Results There was a significant difference between the two treatment groups in operation time (p=0.024), while there was no significant difference in blood loss (p=0.078). No significant difference was found between the two treatment groups in Visual Analog scale (p=0.225), Constant-Murley score (p=0.930), and radiological outcomes (p=0.504). There was linear regression and correlation between Constant-Murley score and age (p=0.027), while there was no linear relationship between status of tuberosity and the function of the shoulder joint (p=0.931). Conclusion We introduce a new operative technique to determine the humerus height and humeral head retroversion for shoulder arthroplasty operation. Based on the data, there is linear regression and correlation between Constant-Murley score and age, while there is no linear relationship between status of tuberosity and the function of the shoulder joint. Our present analyses reveal that our protocol is personalized, easy, effective and feasible.Trial registration: https://doi.org/10.1186/ISRCTN28175069.
Incidence and Predictors of Diabetes Mellitus And Outcome of Pre-Diabetes: A Prospective, Community-Based Cohort Follow-Up Study
Authors: Silva Shamila Thivanshi De , Ediriweera Dileepa , Niriella Madunil Anuk , Beddage Thulani , Kasturiratne Anuradhani , Pathmeswaran Arunasalam , Kato Norihiro , Wickramasinghe Rajitha , Silva H Janaka de 
Published: September 2020

Abstract
BackgroundThere is limited data on prevalence and outcome of prediabetes (PDM) and incidence of type 2 diabetes mellitus (T2DM) from South Asia. We investigated this in an urban, adult population in Sri Lanka that was followed-up for seven years. Methods The study population (selected by age-stratified random sampling from the community) was initially screened in 2007 and re-evaluated in 2014. On both occasions they were assessed by structured interview, anthropometric measurements, liver ultrasound, biochemical and serological tests. Results In the original cohort of 2985 recruited in 2007 [54.8% women, median age (IQR) 53 (47-59)], 737 had T2DM [baseline prevalence 24.7% (95% CI: 23.1–26.2)] and 525 (17.7%) had PDM [54.1% women, median age (IQR) 56 (50-60)]. 2148/2985 (71.6%) attended follow-up in 2014 [57.5% women; median (IQR) 60 (54–66) years], which included 1650 who did not have T2DM in 2007. By 2014, 436/1650 (27.6%) had developed new T2DM [annual incidence 3.9% (95% CI:3.0-4.9). On logistic regression, PDM, central obesity, dyslipidemia and non-alcoholic fatty liver disease (NAFLD) showed significant association with incident T2DM. Of 525 with PDM in 2007, 365 (69.5%) presented for follow up in 2014; 147 (40.3%) remained in PDM, 201 (55.1%) had progressed to T2DM and 17 (4.6%) had reverted to normal. Annual conversion rate of PDM to T2DM was 7.9%. Increase in waist circumference and decrease in HDL predicted progression to T2DM. ConclusionsPresence of components of the metabolic syndrome at baseline predicted new-onset T2DM and conversion of PDM to T2DM. Targeted lifestyle interventions are essential for individuals with metabolic risk to prevent incident T2DM.
Co-evolution of COVID-19 Research and China’s Policies
Authors: Xi Cheng , Li Tang , Maotian Zhou , Guoyan Wang 
Published: September 2020

Abstract
The dynamics of research are a prism that reflects interactions between science, the societal environment, and government policies. Against the backdrop of the ongoing global COVID-19 pandemic, this study explored the changes in, and development of, COVID-19 research in the period from December 30, 2019 to April 27, 2020. The study observes a salient change in research content: an earlier focus on “all patients”, “common symptoms”, and “nucleic acid test” was gradually replaced by a focus on “children”, “pregnant patients”, “severe symptoms”, and a combination of “nucleic acid test” and “antibody assay”. Some topics such as “vaccine R&D”, “knowledge, attitude, and practice” (KAP), and “mental health” were persistent throughout the recent history of China’s COVID-19 research. This study also reveals a correlation between the evolution of COVID-19 policies and the dynamic development of COVID-19 research. In the early stage of the outbreak in China, the formulation of COVID-19 policies followed a rapidly-progressing co-evolutionary model (CEM). The results of this study apply more broadly to the formulation of policies in public health emergencies, especially in the early stages.