bluuebunny/crossref_metadata_2025_split huggingface dataset:
District Health Information System on Maternal, Newborn and Child Health How Good Is It? A Case of Deli Serdang and Sumedang Districts
Authors: Achadi Anhari 
Published: January 2020

Valid data and information are critical for any health programs, in particular for planning, monitoring and evaluation purposes. District Health Information System is designed to produce routine data on process and output type of indicators at district level. This study, taking place at Deli Serdang and Sumedang districts, has its objectives as to learn about the current practice of DHIS, specifically looking at its process and the availability, quality and utilization of thedata. Methods of data collection include in-depth interview with stakeholders at village, health facility and district levels, examination of existing forms at all levels as well as other documents and reports. Findings suggest that weaknesses of DHIS prevail at each level of the system. Complexity of the system has produced inaccurate and suboptimal the use of generated data and information.Key words : District health information system, inaccurate data and informationAbstrakData dan informasi yang valid sangat penting untuk program kesehatan, terutama untuk perencanaan, pemantauan, dan evaluasi. Sistem Informasi Kesehatan Kabupaten dibuat untuk menghasilkan data rutin tentang indikator proses dan output program kesehatan di tingkat kabupaten. Studi ini, yang dilakukan di kabupaten Deli Serdang dan Sumedang, bertujuan untuk mengetahui proses bekerjanya SIK Kabupaten, serta ketersediaan, kualitas dan penggunaan data yang dihasilkan. Pengumpulan data dilakukan dengan menggunakan beberapa cara, yaitu wawancara mendalam dengan para pemangku kepentingan di desa, fasilitas kesehatan, dan kabupaten, pemeriksaan formulir-formulir yang digunakan disemua tingkatan, dokumen lainnya, serta laporan. Temuan studi menunjukkan adanya kelemahan-kelemahan SIK dalam semua tingkatan. Karena kompleknya, sistem menghasilkan data dan informasi yang tidak akurat, dengan tingkat penggunaan yang rendah.Kata kunci : Sistem informasi kesehatan kabupaten, data dan informasi yang tidak akurat
Robotic radical antegrade modular pancreatosplenectomy(RAMPS) versus standard retrograde pancreatosplenectomy (SRPS):study protocol for a randomized controlled trial
Authors: Zhang Gong , Liu Rong , Kang Yuhao , Zhang Haifeng , Wang Fei 
Published: January 2020

Abstract
Background There has been data from meta-analysis suggesting that RAMPS is a safe and effective procedure for adenocarcinoma in the body or tail of the pancreas and is oncologically superior to SRPS. However, previous studies on RAMPS were conducted under the open and laparoscopic surgery. Robotic surgery, on the other hand, plays a role in ergonomics and offers several advantages, including less fatigue, tremor filtering, 7° of wrist-like motion, motion scaling, and three-dimensional vision. At present, there is still a lack of clinical studies to observe the safety and clinical efficacy of Robotic RAMPS in the world. Hence,prospective randomized controlled trials (RCTs) comparing Robotic RAMPS and SRPS are required. We begin a RCT trial to compare short-term surgical and oncological outcomes of Robotic RAMPS and SRPS for patients with distal pancreatectomy.Methods This is a randomized, single-center clinical trial. All included adults are patients with primary carcinoma of the distal pancreatectomy. A total of 246 patients will be randomly allocated to Robotic RAMPS or SRPS. The primary endpoints are oncological outcomes(R0 rate, number of Lymph node). Secondary endpoints are the perioperative complications, Perioperative indicators(operative time,blood loss,blood transfusion volume,costs).Discussion To evaluate the surgical and oncological outcomes of Robotic RAMPS, we therefore undertake a prospective RCT. This procedure may become a standard approach to robotic pancreatosplenectomy.
Dissecting myocardial mechanics in patients with severe aortic stenosis: 2-dimensional vs 3-dimensional-speckle tracking echocardiography
Authors: Bi Xiaojun , Yeung Darwin F , Salah Husam M. , Calle Maria C. Arciniegas , Thaden Jeremy J. , Nhola Lara F. , Schaff Hartzell V. , Pislaru Sorin V. , Pellikka Patricia A. , Pochettino Alberto , Greason Kevin L. , Nkomo Vuyisile T. , Villarraga Hector R. 
Published: January 2020

Abstract
Background: Aortic stenosis (AS) causes left ventricular (LV) pressure overload, leading to adverse LV remodeling and dysfunction. Identifying early subclinical markers of LV dysfunction in patients with significant AS is critical as this could provide support for earlier intervention, which may result in improved long-term outcomes. We therefore examined the impact of severe AS and its consequent increase in LV afterload on myocardial deformation and rotational mechanics by 2-dimensional (2D) and 3-dimensional (3D) speckle-tracking echocardiography.

Methods: We prospectively measured various strain parameters in 168 patients (42% female, mean age 72±12 years) with severe AS and LV ejection fraction (EF) ≥50%, and compared them to normal values found in literature. 2D and 3D images were analyzed for global longitudinal strain (GLS), global circumferential strain (GCS), global radial strain (GRS), basal rotation, apical rotation, and peak systolic twist. We further assessed the degree of concordance between 2D and 3D strain, and examined their association with measures of LV preload and afterload.

Results: Patients with severe AS exhibited significantly lower GLS and GRS but higher GCS, apical rotation, and twist by 2D and 3D echocardiography compared with published normal values (P=0.003 for 3D twist,P<0.001 for all others). Agreement between 2D- and 3D-GLS by concordance correlation coefficient was 0.49 (95% confidence interval: 0.39-0.57). GLS was correlated with valvulo-arterial impedance, a measure of LV afterload (r=0.34, p<0.001 and r=0.23, p=0.003, respectively).

Conclusion: Patients with severe AS demonstrated lower-than-normal GLS and GRS but appear to compensate with higher-than-normal GCS, apical rotation, and twist in order to maintain a preserved LVEF. GLS showed a modest correlation with valvulo-arterial impedance.
Genetic diversity among cultivated beets (Beta vulgaris) assessed via population-based whole genome sequences
Authors: Author) Paul J. Galewski(New Corresponding , Author) J. Mitchell McGrath(Former Corresponding 
Published: January 2020

Abstract
Background:
Diversification on the basis of utilization is a hallmark of Beta vulgaris (beet), as well as other crop species. Often, crop improvement and management activities are segregated by crop type, thus preserving unique genome diversity and organization. Full interfertility is typically retained in crosses between these groups and more traits may be accessible if the genetic basis of crop type lineage were known, along with available genetic markers to effect efficient transfer (e.g., via backcrossing). Beta vulgaris L. (2n =18) is a species complex composed of diverged lineages (e.g., crop types), including the familiar table, leaf (chard), fodder, and sugar beet crop types. Using population genetic and statistical methods with whole genome sequence data from pooled samples of 23 beet cultivars and breeding lines, relationships were determined between accessions based on identity-by-state metrics and shared genetic variation among lineages.
Results:
Distribution of genetic variation within and between crop types showed extensive shared (e.g. non-unique) genetic variation. Lineage specific variation (e.g. apomorphy) within crop types supported a shared demographic history within each crop type, while principal components analysis revealed strong crop type differentiation. Relative contributions of specific chromosomes to genome wide differentiation were ascertained, with each chromosome revealing a different pattern of differentiation with respect to crop type. Inferred population size history for each crop type helped integrate selection history for each lineage, and highlighted potential genetic bottlenecks in the development of cultivated beet lineages.
Conclusions:
A complex evolutionary history of cultigroups in Beta vulgaris was demonstrated, involving lineage divergence as a result of selection and reproductive isolation. Clear delineation of crop types was obfuscated by historical gene flow and common ancestry (e.g. admixture and introgression, and sorting of ancestral polymorphism) which served to share genome variation between crop types and, likely, important phenotypic characters. Table beet was well differentiated as a crop type, and shared more genetic variation within than among crop types. The sugar beet group was not quite as well differentiated as the table beet group. Fodder and chard groups were intermediate between table and sugar groups, perhaps the result of less intensive selection for end use.
Inhibition of JAK2/STAT3/SOCS3 Signaling Attenuates Atherosclerosis in Rabbit
Authors: Yang Xilan , Jia Jian , Yu Zhen , Duanmu Zheng , He Huiwei , Chen Sen , Qu Chen 
Published: January 2020

Abstract
Background : Previous studies have indicated that the JAK/STAT signaling pathway is involved in modulating arterial adventitia inﬂammation response. In this study, we designed experiment to further investigate the effect of JAK2/STAT3/SOCS3 signaling in rabbit atherosclerosis process.
Methods : Atherosclerosis was induced in the abdominal arteries of rabbits by atherogenic diet and endothelial damage. At the same time, animals were received either no treatment or ruxolitinib and killed after 12 weeks.
Results : H&E staining and CT scan analysis showed that rabbit atherosclerosis model was constructed successfully. Ruxolitinib, an inhibitor of the Janus kinase 2 (JAK2), substantially decreased the area of atherosclerotic plaques in rabbits treated with high fat diet and balloon injury of the aorta. Moreover, ruxolitinib substantially decreased IL-6 and TNF-α, but increased IL-10 and IL-17 levels in plasma of atherosclerotic rabbits. Additionally, ruxolitinib reduced TC and TG contents and AIP, while enhanced HDL-C level in rabbit plasma. Furthermore, we found that JAK2 and STAT3 phosphorylation were upregulated in rabbits with atherosclerosis when compared with that of the control group, and furthermore the expression of SOCS3 was also increased due to the activation of JAK2 and STAT3, while ruxolitinib inactivated JAK2 and STAT3 pathway and decreased SOCS3 expression.
Conclusion : Taken together, inhibiting the JAK2/STAT3/SOCS3 signaling pathway may be a novel method for the clinical treatment of artery atherosclerosis.
Prevention of the adverse effects of androgen deprivation therapy for prostate cancer using a 12-month home-based progressive resistance training program: a randomised controlled study
Authors: Lam Teresa , Cheema Birinder , Hayden Amy , Lord Stephen R. , Gurney Howard , Gounden Shivanjini , Reddy Navneeta , Shahidipour Haleh , Read Scott , Stone Glenn , McLean Mark , Birzniece Vita 
Published: January 2020

Abstract
The authors have withdrawn this preprint from Research Square
MHC associations of ankylosing spondylitis in East Asians are complex and involve non-HLA-B27 HLA contributions
Authors: Wang Geng , Kim Tae-Hwan , Li Zhixiu , Cortes Adrian , Kim Kwangwoo , Bang So-Young , Leo Paul , Brown Matthew , Xu Huji 
Published: January 2020

Abstract
Background The association of HLA-B*27 with AS is amongst the strongest of any known association of a common variant with any human disease. Nonetheless, there is strong evidence indicating that other HLA-B alleles are involved in the disease. Large studies in European case-control cohorts have demonstrated risk associations with HLA-B*40 and multiple other HLA-B, -A and Class II alleles, and demonstrated that in that ethnic group the amino acid sequence at position 97 in HLA-B is the key determinant of HLA associations with AS. A recent study in Korean AS cases and controls additionally identified association at HLA-C*15:02. In the current study we examined the MHC associations of AS in an expanded east Asian cohort.Methods 1,637 Chinese, Taiwanese and Korean AS cases meeting the modified New York Criteria for AS, and 1,589 ethnically matched controls, were genotyped with the Illumina Immunochip, including a dense coverage of the MHC region. HLA genotypes and amino acid composition was imputed using the SNP2HLA program using the Han-MHC reference panel based on the data of Han Chinese subjects (largest Asian MHC reference available (n= 9,689)), and association tested using logistic regression using 10 principal components to control for population stratification effects.Results Strong association was seen with HLA-B*27 (odds ratio (OR) =205.3, P=5.764×10 -244 ). Controlling for this association, the strongest risk association is seen with HLA-C*15 at genome-wide significant level (OR = 7.62, P=9.30×10 -19 ), and confirmed association is also seen with HLA-B*40 at suggestive level (OR= 1.65, P= 2.54x10 -4 ). At amino acid level the strongest association seen in uncontrolled analysis was with histidine at position 114 in HLA-B (p=7.24 x10 -241 ), but conditional analyses suggest that the primary amino acid associations are with lysine at position 70 and asparagine at position 97. Restriction of the ERAP1 association with HLA-B27-positive AS, previously reported in European subjects, was confirmed in East Asians.Conclusions This study confirms in East Asians that the HLA associations of AS are multiple, including previously reported associations at HLA-B*27, -B*40, -C*15 , and –DRB1*01 , as well as novel associations with –DRB1*13 and –B*39 . The HLA-B associations are driven by the amino acids at positions 70 and 97, in the B-pocket of HLA-B.
Evidence-Based Health In Primary Care: Among Electronic Portals, Telehealth And Digital Libraries
Authors: Costa José Roberto Bittencourt , Alves Luiz Anastacio , Alberto Anael Viana Pinto , Souza Cristina Alves Magalhães de 
Published: January 2020

Abstract
Background: To make an analysis and propose solutions to improve the teaching and learning process in the scenario of the Basic Family Health Units (BFHU). Method: First phase: Authors conducted a qualitative-quantitative study with students and preceptors of Primary Care containing open and closed questions from medical schools. The closed questions were presented as descriptive statistics and the open ones from the creation of categories. It was pointed out the perception of the main problems for teaching learning. Second phase: Meetings were held from students and respective preceptor for 6 months, using electronic portals, through the application of new questionnaires, using the Likert scale in pre and post evaluation. Results: On the first phase, 40% of the students considered as a problem the degree of training of the local preceptor. A similar result was found in the speech of the teacher, which categorization revealed a lack of adequacy between training and performance in Primary Care in most of the categories created. Based on our results, we used the BHFU to apply new strategies little used for teaching and learning, in order to improve health practices, such as the use of the Evidence Based Health portal, which has shown greater navigability and offers studies with clinical evidence criteria. On the second phase, the authors identified an improvement in the quality of learning both by the preceptor and by the students. Conclusions: The use of electronic portals can be a tool that legitimized or enhance the teaching and learning (teaching/learning space) in BHFU.
Role of HPV 16 variants among cervical samples from Northeastern Brazil
Authors: Silva Rodrigo Lopes da , Batista Zulmira da Silva , Bastos Gerusinete Rodrigues , Cunha Ana Paula Almeida , Figueiredo Fábio Vidal , Castro Lailson Oliveira de , Pereira Liwerbeth dos Anjos , Silva Marcos Antonio Custódio Neto da , Vidal Flávia Castello Branco , Barros Maria Claudene , Fraga Elmary da Costa , Brito Luciane Maria Oliveira , Barbosa Maria do Carmo Lacerda , Moreira Miguel Ângelo Martins , Nascimento Maria do Desterro Soares Brandão 
Published: January 2020

Abstract
Background: Cervical cancer is the fourth most common type of cancer affecting women globally. In Brazil, it is the third most frequent type of cancer in women and HPV is present in approximately 90% of cases. Evidence suggests that variants of HPV 16 can interfere biologically and etiologically during the development of cervical cancer. Methods: Cervix tumor fragments were collected, their DNA was extracted, and nested PCR was used to detect HPV. Positive samples were sequenced to determine the viral genotype. To characterize the HPV 16 strains, positive samples PCR was used to amplify the LCR and E6 regions of the HPV 16 virus.Results: Data from 120 patients with cervical cancer were analyzed. Most women were between 41 and 54 years of age, had schooling until primary school, a family income between 1 and 2 times the minimum wage and were married/in a consensual union. There was no statistically significant association between HPV or socio-demographic variables and risk factors for cervical cancer (P < 0.05). HPV was present in 88 women (73%). The most prevalent types were HPV 16 (47/54%), HPV 18 (12/13.8%), HPV 35 (6/6.9%) and HPV 45 (5/5.7%). Of the 47 HPV 16 positive cases, variant A (49%) was present in 23 samples, followed by variant D in 20 cases (43%), and variants B and C in 2 cases each (4%).The most prevalent histological type of HPV 16 tumors was epidermoid carcinoma, followed by adenocarcinoma. There was a statistically significant association between HPV 16 variants and the tumors’ histological types (P < 0.001).Conclusions: Knowledge of HPV 16 variants will provide data on their influence on the pathological and oncogenic aspects of cervical lesions.
Integrated analysis of differentially expressed long noncoding RNAs and mRNAs associated with high-fat diet-induced hepatic insulin resistance in mice
Authors: zhou zengyuan , Zhao Xue , Chen Liang , Li Yuzheng , Chen Zhao , Wang Yuanyuan , Zhou Zihao , Chu Xia 
Published: January 2020

Abstract
Background Hepatic insulin resistance (IR) is an early pathological characteristic of many metabolic diseases, such as type 2 diabetes. Long noncoding RNAs (lncRNAs) have been identified as mediators of IR and related diseases. However, the roles of lncRNAs in hepatic IR remain largely unknown.Method High-throughput sequencing was performed on liver tissue samples from normal diet (ND)-fed mice and high-fat diet (HFD)-induced hepatic IR mice. lncRNAs and mRNAs that were differentially expressed (DE) between the two groups were identified by bioinformatic analyses. Seven DE lncRNAs were validated by quantitative real-time PCR (q-PCR). The potential functions of the DE lncRNAs were predicted by Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses of target genes. In addition, integrated analysis was performed for the DE lncRNAs and mRNAs to predict their interaction relationships.ResultsA total of 232 DE lncRNAs were identified in the HFD-induced hepatic IR mice compared with the ND-fed mice. These DE lncRNAs included 108 upregulated and 124 downregulated lncRNAs, and 7 of the DE lncRNAs were validated by q-PCR. In addition, 291 DE mRNAs including 166 upregulated and 125 downregulated mRNAs were identified in the HFD group. Furthermore, target genes of the DE lncRNAs were predicted, and functional enrichment results showed that the enriched genes were involved in IR- and glycolipid metabolism-related processes. Additionally, the coexpression network was also constructed to further reflect the potential functions of the DE lncRNAs.Conclusion The study describes the expression profiles of lncRNAs and mRNAs and the functional networks involved in HFD-induced hepatic IR. These findings may provide a new perspective for the study of lncRNAs in hepatic IR- and glycolipid metabolism-related diseases.
Gene Expression Pattern of PI3K/AKT Pathway Based on Insulin Resistance and Vitamin D Toward the Understanding of T2DM Pathogenesis
Authors: Khorami Somayeh Alsadat Hosseini , Mutalib Mohd Sokhini Abd , Shiraz Mohammad Feili , Abdullah Joseph Anthony , Rejali Zulida , Ali Razana Mohd , Khaza'ai Huzwah 
Published: January 2020

Abstract
Insulin-stimulated glucose transport occurs via PI3K/AKT -dependent pathway which results in GLUT4 translocation from intracellular vesicles to plasma membrane and glucose uptake. PTEN , as a phosphatase, is the main antagonist of the PI3K/AKT pathway’s kinases. Present study was performed to investigate underlying mechanism responsible for defects in insulin signalling, hence, RT-PCR was employed to investigate mRNA expression level of IRS1/PI3K/PDK1/AKT2/GLUT4/PTEN in diabetic and non-diabetic participants and serum vitamin D was measured by HPLC. Findings provide evidence that IRS1 gene expression was preserved while PI3K/PDK1/AKT2/GLUT4 were expressed significantly lower in diabetics compared to non-diabetics. Albeit there was no significant difference in PTEN expression between groups, PTEN was up-regulated by the years of having diabetes. As T2DM has been characterized by defects in insulin signalling at transcriptional level and post-translational modifications, it is difficult to conclude what exactly happens since only gene expression was considered, nevertheless it can be concluded that insulin resistance is not caused through an alteration in PTEN expression as a primary defect but may be caused by decreased PI3K/PDK1/AKT2/GLUT4 signalling and dysregulation of feedback loops. Particularly, PTEN expression showed a significant relation with duration of diabetes, suggesting that PTEN may not be the cause of the reduced expression of PI3K/AKT pathway in diabetes while it can be the effect of that. No significant correlations between serum vitamin D concentration and gene expression level of GOIs were observed in either group of participants which could be due to the non-linearity relationships as insulin signaling is a cascade with amplifying properties.
Family physician program and burden of referral for outpatient visit in Iran: A multicenter cross-sectional study protocol
Authors: Sokhanvar Mobin , Kabir Mohammad Javad , Bevrani Hossein , Nosratnejad Shirin , Janati Ali , Arab-zozani Morteza , Hasanpoor Edris 
Published: January 2020

Abstract
Background : Primary healthcare (PHC) developed around the world with a focus on the family physician, which can improve access and quality of services. The study will estimate the burden of referral for outpatients visit through a family physician program (FPP) in Iran. Methods : This will be a descriptive-analytical cross-sectional study. The data for self-referrals and those who are officially referred to the specialized level of outpatient care will collect from the rural and urban populations in four provinces. Of these provinces, two are the pilot sites of urban FPP and the other two provinces are matched provinces. Patient recruitment will be carried out by a research assistant located in each health network of the province. We will collect the data on outpatient visits, prescribed medicine, medical imaging, and laboratory services. Descriptive and analytical statistics will be generated to estimate the burden of referral to outpatient visits among and between provinces. Discussion : This study has been approved by the Ethics committee of Tabriz University of Medical Sciences. The results of this study will be the first national evidence of the effectiveness of FPP and the burden of referral for outpatients visit in Iran. One of the main strategies to improve access, quality and efficiency of healthcare systems is a family physician program (FPP) as the primary care provider within an appropriate referral system. Evidence suggests that health systems, with a focus on PHC, have had positive consequences for health outcomes, healthcare quality, access to and continuity of health care, system efficiency and financial sustainability and public satisfaction.