bluuebunny/crossref_metadata_2025_split huggingface dataset:
Disease-specific plasma levels of mitokines FGF21, GDF15, and Humanin in type II diabetes and Alzheimer’s disease in comparison with healthy aging
Authors: Conte Maria , Sabbatinelli Jacopo , Chiariello Antonio , Martucci Morena , Santoro Aurelia , Monti Daniela , Arcaro Marina , Galimberti Daniela , Scarpini Elio , Bonfigli Anna Rita , Giuliani Angelica , Olivieri Fabiola , Franceschi Claudio , Salvioli Stefano 
Published: October 2020

AbstractFibroblast Growth Factor 21 (FGF21), Growth Differentiation Factor 15 (GDF15), and Humanin (HN) are mitochondrial stress-related mitokines, whose role in health and disease is still debated. In this study, we confirmed that their plasma levels are positively correlated with age in healthy subjects. However, when looking at patients with type 2 diabetes (T2D) or Alzheimer’s disease (AD), two age-related diseases sharing a mitochondrial impairment, we found that GDF15 is elevated in T2D but not in AD and represents a risk factor for T2D complications, while FGF21 and HN are lower in AD but not in T2D. Moreover, FGF21 reaches the highest levels in centenarian’ offspring, a model of successful aging. As a whole, these data indicate that (i) the adaptive mitokine response observed in healthy aging is lost in age-related diseases, (ii) a common expression pattern of mitokines does not emerge in T2D and AD, suggesting an unpredicted complexity and disease-specificity, and (iii) FGF21 emerges as a candidate marker of healthy aging.
Preliminary study on mechanical characteristics of maxillofacial soft and hard tissues for virtual surgery
Authors: Zhuang Yu , Chen Jie , Liu Qingcheng , Zou Fan , Lin Yuheng , An Qinglong , Yu Hongbo 
Published: October 2020

Abstract
Purpose
Virtual surgery system can provide us a realistic and immersive training environment, in which haptic force-feedback gives operators ‘touching feeling.’ Appropriate deformation models of soft and hard tissues are required for the achievement of real-time haptic feedback. To improve accuracy of modeling and haptic feedback simulation for maxillofacial virtual surgery, mechanical characteristics of soft and hard tissues should be explored.

Methods
Craniofacial soft tissues from one male and female cadavers were divided into two layers: skin and muscle. Maxillofacial tissues were divided into frontal, chin, temporalis, masseter regions. Insertion and cutting process were conducted using VMX42 5-axis linkage system and recorded by piezoelectric dynamometer. Maximum stiffness values were analyzed, and insertion curves before puncture were fitted using a polynomial model. Elasticity modulus and hardness of maxillofacial hard tissues were measured and analyzed using Berkovich nanoindentation.

Results
Tissues in different maxillofacial regions, as well as from different layers (skin and muscle), displayed various mechanical performance. Maximum stiffness values and cutting force of soft tissues in male and female had significant difference. The third-order polynomial was demonstrated to fit the insertion curves well before puncture. Furthermore, elasticity modulus and hardness of enamel were significantly greater than that of zygoma, maxilla and mandible.

Conclusion
Mechanical properties of hard tissues are relatively stable, which can be applied in virtual surgery system for physical model construction. Insertion model and cutting force for soft tissues are meaningful and applicable and can be utilized to promote the accuracy of response for haptic feedback sensations.
Ex vivo Induction of Apoptotic Mesenchymal Stem Cell by High Hydrostatic Pressure
Authors: Le Tien Minh , Morimoto Naoki , Ly Nhung Thi My , Mitsui Toshihito , Notodihardjo Sharon Claudia , Ogino Shuichi , Arata Jun , Kakudo Natsuko , Kusumoto Kenji 
Published: October 2020

AbstractAmong promising solutions for tissue repair and wound healing, mesenchymal stem (or stromal) cells (MSCs) have been a focus of attention and have become the most clinically studied experimental cell therapy. Recent studies reported the importance of apoptosis in MSC-mediated immunomodulation, in which apoptotic MSCs (apoMSCs) were shown to be superior to living MSCs. Nowadays, high hydrostatic pressure (HHP), a physical technique that uses only fluid pressure, has been developed and applied in various bioscience fields, including biotechnology, biomaterials, and regenerative medicine, as its safe and simply operation. In the current study, we investigated the impact of HHP treatment on human bone marrow-MSC survival and proliferation. Based on the detection of executioner caspase activation, phosphatidylserine exposure, DNA fragmentation (TUNEL) and irrefutable ultrastructural morphological changes on transmission electron microscopy (TEM), our data revealed that HHP treatment induced complete apoptosis in MSCs. Notably, this technique might provide manipulated products for use in cell-based therapies as manufacturing capability expands. We hope that our findings will contribute to the improvement of MSCs or EVs in translational research development.
Could the grasses have played a role in the earliest salt exploitation? Phytoliths analysis of prehistoric salt spring from Hălăbutoaia - Ţolici (Romania)
Authors: Danu Mihaela , Delhon Claire , Weller Olivier 
Published: October 2020

AbstractThe salt spring exploitation fromHălăbutoaia- Ţolici (Neamț County, Romania) dates back to the Early Neolithic and lasted throughout Chalcolithic. The deposit stratigraphy is estimated at 8 m and covers 2500 years of history (c. 6000–3500 BCE). In order to document the possible use of plants, particularly of the grasses, in the salt production process, we realized a detailed study of phytoliths preserved in several archaeological levels ofHălăbutoaiasite. The most identified morphotypes come from grass family. Analysis revealed an important representation of inflorescence bracts phytoliths (especially Elongate dendritic) showing the anthropogenic origin of the assemblages. Bilobateare also well represented. These forms attest the presence of wild panicoid grasses (e.g. wild millet) and/or cultivated millets in the area. In some very punctual assemblages, forms produced by dicots are well represented. Considering the low phytoliths production by dicots plants, it involves a special accumulation of these types of plants that could be related to woody plants used as fuel for salt production. It is very possible that grasses had a very important role in pyrotechnology used to produce salt, either as the main fuel or as a mean for controlling the temperature or even as a firelighter.
Vulnerability assessment considering impact of future groundwater exploitation on coastal groundwater resources in northeastern Jeju Island, South Korea
Authors: Chang Sun Woo , Chung Il-Moon , Kim Min-Gyu , Yifru Bisrat Ayalew 
Published: October 2020

AbstractJeju Island is the largest island in South Korea. Recently, extensive groundwater abstraction has been reported from the shallow aquifer in the northeast region of the island. This study simulated the freshwater resources of the aquifer to estimate the sustainability of groundwater use on Jeju Island in terms of its vulnerability to seawater intrusion. Three-dimensional finite-difference numerical groundwater models were simulated using the MODFLOW-family code SEAWAT. Precise and recent groundwater level and multi-depth salinity data obtained from the study site were used for model calibration; the simulated results showed good agreement with the observed data. SEAWAT was used to delineate the current seawater-freshwater interface to quantitatively estimate the coastal fresh groundwater resources. Future stress scenarios were also simulated in response to increased pumping and various changes in the recharge. The results showed that current groundwater use in the coastal aquifer did not induce seawater intrusion in the coastal aquifer, but seawater intrusion will occur if the dry season continues for the next ten years. The vulnerability assessment based on the predicted groundwater levels and ion concentrations using numerical simulations suggests future vulnerability in the aquifer; therefore, continuous assessment and visualization of the aquifer sustainability is vital. Future projections by the integrated SEAWAT simulation and GALDIT assessment showed that an increase in groundwater pumping may escalate the vulnerability status of coastal groundwater resources from moderate to high in some areas of the study site, by inducing lateral seawater intrusion in deeper areas of the unconfined aquifer.
Evaluation of drilling muds enhanced with modified starch for HPHT well applications
Authors: Sulaimon Aliyu Adebayo , Akintola Sarah Abidemi , Mohd Johari Mohd Adam Bin , Isehunwa Sunday Oloruntoba 
Published: October 2020

AbstractThe use of carboxymethyl cellulose (CMC) in oil and gas well drilling operations has improved the filtration loss and mud cake properties of drilling muds. The introduction of starch has also reduced, for example, the viscosity, fluid loss, and mud cake properties of the drilling fluids. However, normal starch has some drawbacks such as low shear stress resistance, thermal decomposition, high retrogradation, and syneresis. Hence, starch modification, achieved through acetylation and carboxymethylation, has been introduced to overcome these limitations. In this study, modified starches, from cassava and maize, were used to enhance the properties of water-based muds under high-pressure high temperature (HPHT) conditions, and their performances were compared with that of the CMC. The mud samples added with acetylated cassava or maize starch exhibited the smallest filtrate volumes and filtrate losses within the American Petroleum Institute specification. Therefore, these modified starch-added muds could replace CMC as fluid loss agents since, unlike it, they can withstand HPHT conditions.
Cytogenetic investigation of Arctic char × brook trout F1, F2 and backcross hybrids revealed remnants of the chromosomal rearrangements
Authors: Pomianowski Konrad , Ocalewicz Konrad 
Published: October 2020

AbstractArctic char (Salvelinus alpinus) and brook trout (Salvelinus fontinalis) hybridize and their offspring is viable and fertile. This may be a real treat for the native European stocks of Arctic char which gene pools might be unintendedly contaminated with the genetic elements of brook trout. On the other hand, hybrids of these two species are appreciated by customers and have some potential for the aquaculture. Moreover, Salvelinus hybrids and backcross individuals are interesting models in the research focused on influence of hybridization on the genomic organization and chromosome rearrangements. Thus, the main goal of the present study was to examine chromosomes of Arctic char × brook trout F1, F2 hybrids and backcross individuals and compare with genomic information concerning parental species to recognize karyotypic changes provoked by the hybridization events. Application of conventional and molecular (FISH) techniques allow to identify characteristic chromosomes for both parental species in the hybrid progeny and show multiplicity of cytotypes among different types of crosses with variability in structure and number of chromosome (81–85) and chromosome arm (99–101). Chromosome fragment was detected in the karyotype of one F1 and one backcross individual and the presence of one triploid (3n) fish was documented. Occurrence of chromosomes containing internally located telomeric sequences (ITS) inherited after brook trout or both parental species was shown in F1 and backcross progeny. Moreover, additional CMA3-positive signal on chromosome from Arctic char pair no. 2 in F1 fish and interstitially located active NOR visible on subtelo-acrocentric (F2 hybrid) and acrocentric (Sf × H individual) chromosomes were detected. Described polymorphic chromosomes together with specific, interstitial location of CMA3-positive found in F2 and Sf × H hybrids and DAPI-positive regions observed in H × Sa fish at different uniarmed chromosomes pair presumably are remnants of chromosomal rearrangements. Provided results strongly indicate that the hybridization process influenced the genome organization in the Salvelinus hybrid progeny.
Acid-assisted extraction and hydrolysis of inulin from chicory roots to obtain fructose-enriched extracts
Authors: Stökle Katrin , Jung Dennis , Kruse Andrea 
Published: October 2020

AbstractChicory (Cichorium intybus var. foliosum) roots are an agricultural residue and a low cost feedstock for the production of the platform chemical 5-Hxdroxymethylfurfural (HMF). In a first step, inulin and fructose have to be extracted from the roots. The resulting aqueous extract represents the starting material for the HMF production. In the reaction to HMF, inulin has to be hydrolyzed first to fructose. For this reason, two methods to increase the fructose content in these extracts before the reaction were investigated. This was conducted within the framework of integrating acid hydrolysis into a biorefinery process for HMF production. The first method (one-step process) was acid-assisted extraction to directly hydrolyze inulin in the course of the extraction process. Chicory roots were extracted at 60 and 80 °C at pH 2 and 4 using buffer solutions. The second approach (two-step process) was aqueous extraction at neutral pH followed by nitric acid hydrolysis of the extract at 60 and 80 °C under reduced pH. It was found that in the first approach, the pH of 2 led to a fivefold increase in the fructose content of the extract, resulting from inulin hydrolysis and corresponding to 56% of theoretical fructose yield. For the second approach, it was possible to achieve complete hydrolysis at pH below 2.5 and at 80 °C. Separating extraction and hydrolysis was found to be more suitable in terms of including this process step into a biorefinery concept for HMF production. It was possible to reduce the initial inulin content by 95%.
Flame retardant and durable chloroprene rubber and styrene-butadiene rubber blends crosslinked with copper(I) oxide
Authors: Kobędza Piotr , Smejda-Krzewicka Aleksandra , Olejnik Anna , Strzelec Krzysztof 
Published: October 2020

AbstractThe purpose of this work was to investigate the effect of application of copper(I) oxide (Cu2O) as an unconventional crosslinking agent of chloroprene (CR) and styrene-butadiene (SBR) rubber compositions. The use of Cu2O arises from the need to limit the application of ZnO as a CR crosslinking agent. The obtained results indicate that CR/SBR blends crosslinked with Cu2O are characterized by good mechanical properties and a high degree of crosslinking The results show that the proportion of both processing rubbers, as well as the amount of copper(I) oxide, influence the crosslinking of CR/SBR blends and the properties of the vulcanizates. Performing FTIR analysis has allowed the development of a crosslinking mechanism. Crosslinking presumably takes place according to the mechanism of Friedel–Crafts alkylation reaction. Silica, chalk, china clay and nanofiller (montmorillonite modified with quaternary ammonium salt containing hydroxyl groups) were applied as fillers. Among the fillers, silica had the greatest impact on improving the properties. It is arisen from silica activity, unlike other used fillers. The AFM analysis allowed us to determine the miscibility of the rubbers and dispersion of fillers. Thermal analysis was performed to determine the changes occurring as a result of material heating. The low intensity of the peaks corresponding to the crosslinking of the CR/SBR blends may indicate a small amount of bonds formed during heating, or possibility is the formation of connections between chains with a low binding energy. The use of chalk, china clay or silica increases the thermal stability of the vulcanizates. Obtained vulcanizates were characterized by increased incombustibility. The study of combustion time in the air showed that the prepared vulcanizates did not support the burning.
Heart Failure Diagnosis, Readmission, and Mortality Prediction Using Machine Learning and Artificial Intelligence Models
Authors: Guo Aixia , Pasque Michael , Loh Francis , Mann Douglas L. , Payne Philip R. O. 
Published: October 2020

Abstract
Purpose of Review
One in five people will develop heart failure (HF), and 50% of HF patients die in 5 years. The HF diagnosis, readmission, and mortality prediction are essential to develop personalized prevention and treatment plans. This review summarizes recent findings and approaches of machine learning models for HF diagnostic and outcome prediction using electronic health record (EHR) data.

Recent Findings
A set of machine learning models have been developed for HF diagnostic and outcome prediction using diverse variables derived from EHR data, including demographic, medical note, laboratory, and image data, and achieved expert-comparable prediction results.

Summary
Machine learning models can facilitate the identification of HF patients, as well as accurate patient-specific assessment of their risk for readmission and mortality. Additionally, novel machine learning techniques for integration of diverse data and improvement of model predictive accuracy in imbalanced data sets are critical for further development of these promising modeling methodologies.
Electrostatic attraction caused by triboelectrification in climbing geckos
Authors: Song Yi , Wang Zhouyi , Li Yang , Dai Zhendong 
Published: October 2020

AbstractAdhesion achieved through feet setae is fundamental for gecko agilely maneuvering. Although diverse hypotheses have been proposed, none of them thoroughly explains the setae function, implying a kind of hybrid-mechanism-based adhesion in geckos. In addition to van der Waals interactions and capillary force, the electrostatic attraction that emerges from triboelectrification was suggested as a component of setae adhesion. Nevertheless, the contribution by electrostatic attraction to the total setae attachment is still controversial. In this study, we analyzed the occurrence of electrostatic attraction at gecko setae through experiments and model analyses. By touching the substrates with only ∼1/70th of the foot area, freely wall-climbing geckos developed tribocharge at their feet setae with a density of ∼277 pC/mm2, generating electrostatic attractions with a strength of ∼4.4 mN/mm2. From this perspective, the adhesion driven by triboelectrification could account for about 1% of total adhesion. Model analyses at spatula level indicated a similar result showing that the electrostatic force might account for ∼3% of the adhesion that facilitates wall-climbing in geckos. The low contribution of the electrostatic force partly explains why geckos always face difficulty in maneuvering onto those substrates (e.g., teflon) where they could easily develop tribocharge but difficultly generate van der Waals force. However, long-range electrostatic forces may play other roles in a distance range where the van der Waals interaction cannot function. These findings not only add to our understanding of the mechanism of gecko adhesion, but also will help us advance gecko-inspired fibular adhesives.
Coalitions and coordination in Washington think tanks: board interlock among Washington D.C.-based policy research and planning organizations
Authors: Furnas Alexander C. 
Published: October 2020

AbstractThink tanks have become central players in the political and policy ecosystem of the United States, yet the communication and coordination strategies and connections between them remain relatively unexamined. This paper begins to remedy that by using IRS 990 data to construct and analyze the largest board interlock network among policy planning organizations to date. An analysis of 277 policy research organizations located in Washington D.C. reveals high levels of resource seeking, issue-area coordination and weak evidence of ideological coordination among these substantively important organizations in American politics.
Observations of the Low‐Mode Internal Tide and Its Interaction With Mesoscale Flow South of the Azores
Authors: Löb Jonas , Köhler Janna , Mertens Christian , Walter Maren , Li Zhuhua , von Storch Jin‐Song , Zhao Zhongxiang , Rhein Monika 
Published: October 2020

AbstractUnderstanding the temporal variability of internal tides plays a crucial role in identifying sources and sinks of energy in the ocean. Using a 10‐month‐long time series from moored instruments inside a tidal beam south of the Azores, the magnitude and the underlying causes of temporal variability in the first two modes of the internal tide energy flux was studied. We analyzed changes of the direction and coherence of the energy flux, its modal structure, and the impact of two eddies. Semidiurnal energy fluxes were further compared with estimates from a 1/10° ocean global circulation model, as well as with fluxes derived from satellite altimetry. All energy fluxes correlate reasonably well in direction, deviations from its fixed phase relation to astronomical forcing, and modal composition while model and satellite underestimate the total energy flux. A pronounced damping of the in situ fluxes coincides with the passing of two eddies. In the presence of a surface‐intensified eddy, the coherent part of the energy flux in the first two modes is lowered by more than 40%, a subsurface eddy coincides with a decrease of the energy flux mainly in the second mode. These observations support the hypothesis that eddy interactions increase the incoherent part of the energy flux and transfer energy from low modes into higher modes, which can lead to increased local dissipation. It remains an open question how much of the energy converted from lower to higher modes results in local dissipation, a crucial part in creating energetically consistent ocean‐climate models.
Estimation of Permafrost SOC Stock and Turnover Time Using a Land Surface Model With Vertical Heterogeneity of Permafrost Soils
Authors: Shu Shijie , Jain Atul K. , Koven Charles D. , Mishra Umakant 
Published: October 2020

AbstractWe developed vertically resolved soil biogeochemistry (carbon and nitrogen) module and implemented it into a land surface model, ISAM. The model captures the vertical heterogeneity of the northern high latitudes permafrost soil organic carbon (SOC). We also implemented Δ14C to estimate SOC turnover time, a critical determinant of SOC stocks, sequestration potential, and the carbon cycle feedback under changing atmospheric CO2 concentration [CO2] and climate. ISAM accounted for the vertical movement of SOC caused by cryoturbation and its linkage to frost heaving process, oxygen availability, organo‐mineral interaction, and depth‐dependent environmental modifiers. After evaluating the model processes using the site and regional level heterotrophic respiration, SOC stocks, and soil Δ14C profiles, the vertically resolved soil biogeochemistry version of the model (ISAM‐1D) estimated permafrost SOC turnover time of 1,443 years, which is about 3 times more than the estimation based on the without vertically resolved version of ISAM (ISAM‐0D). ISAM‐1D‐simulated SOC stocks for permafrost regions was 319 Pg C in the top 1 m soil depth by the 2000s, about 80% higher than the estimates based on ISAM‐0D. ISAM‐1D SOC stock and turnover time were compared well with the observations. However, the longer SOC turnover time preserves less SOC stocks due to the lower carbon use efficiency (CUE) for SOC than ISAM‐0D and thus respires more SOC than being transferred downward by cryoturbation. ISAM‐1D simulated reduced SOC sequestration (3.7 Pg C) compared to ISAM‐0D (4.8 Pg C) and published Earth system models (ESMs) over the 1860s–2000s, due to weaker [CO2]‐carbon cycle and stronger climate‐carbon cycle feedbacks, highlighting the importance of the vertically heterogeneous soil for understanding the permafrost SOC sinks.