bluuebunny/crossref_metadata_2025_split huggingface dataset:
Understanding Interdependent Climate Change Risks Using a Serious Game
Authors: Undorf Sabine , Tett Simon F. B. , Hagg Joseph , Metzger Marc J. , Wilson Chris , Edmond Graham , Jacques-Turner Miranda , Forrest Stuart , Shoote Marion 
Published: February 2020

AbstractAnthropogenic climate change calls for rapid and enormous cuts in emissions of CO2 and other greenhouse gases to mitigate future impacts. Even with these, however, many changes will continue to occur over the next 20–30 years adding to those already observed. Adaptation is crucial and urgent, but identifying strategies is complex and requires dialogue and cooperation among stakeholders, especially for infrastructure that exhibits interdependent risks in that failure in one type may impact others. A serious game was codeveloped with infrastructure operators to communicate climate projections and climate hazards to them; identify potential interdependencies, cascading impacts, cumulative effects, and vulnerability hot spots; and engage them to improve cooperation and enable a shared understanding of cross-cutting climate risks and interdependencies. In the game, players provide present-day infrastructure services in the Inverclyde district, Scotland, as they experience a plausible decade of 2050s weather characterized by a sequence of hazard events. This sequence was extracted from climate model projections to ensure scientific plausibility. The infrastructure operators were responsible for drinking water and gas supplies, road and rail transport, wastewater treatment, and civil infrastructure. When playing the game the participating U.K. infrastructure providers felt that although there were challenges, they could cope with 2050s climate change. None of the projected hazard events were anticipated to cause catastrophic impact cascades on infrastructure. The game was positively received, and the study suggests it is a useful tool to both communicate climate hazards and explore potential interdependent risks by bringing together stakeholders’ individual expertise in an engaging way.
Convective Dynamics and the Response of Precipitation Extremes to Warming in Radiative–Convective Equilibrium
Authors: Abbott Tristan H. , Cronin Timothy W. , Beucler Tom 
Published: February 2020

AbstractTropical precipitation extremes are expected to strengthen with warming, but quantitative estimates remain uncertain because of a poor understanding of changes in convective dynamics. This uncertainty is addressed here by analyzing idealized convection-permitting simulations of radiative–convective equilibrium in long-channel geometry. Across a wide range of climates, the thermodynamic contribution to changes in instantaneous precipitation extremes follows near-surface moisture, and the dynamic contribution is positive and small but is sensitive to domain size. The shapes of mass flux profiles associated with precipitation extremes are determined by conditional sampling that favors strong vertical motion at levels where the vertical saturation specific humidity gradient is large, and mass flux profiles collapse to a common shape across climates when plotted in a moisture-based vertical coordinate. The collapse, robust to changes in microphysics and turbulence schemes, implies a thermodynamic contribution that scales with near-surface moisture despite substantial convergence aloft and allows the dynamic contribution to be defined by the pressure velocity at a single level. Linking the simplified dynamic mode to vertical velocities from entraining plume models reveals that the small dynamic mode in channel simulations (2% K−1) is caused by opposing height dependences of vertical velocity and density, together with the buffering influence of cloud-base buoyancies that vary little with surface temperature. These results reinforce an emerging picture of the response of extreme tropical precipitation rates to warming: a thermodynamic mode of about 7% K−1 dominates, with a minor contribution from changes in dynamics.
The different expression of glycogen phosphorylases in renal clear cell renal carcinoma and chromophobe renal carcinoma
Authors: Lu Yang , Luo Guangda , Zhu Songbiao , Wang Xu , Chen Yuling , Dong ZhouHuan , Wang Shiyu , Ma Jie , Deng Haiteng , Wu Di , Dong Jun 
Published: February 2020

Abstract
Background
The various pathogenesis between Clear cell renal carcinoma (CCRCC) and Chromophobe renal carcinoma (CHRCC) contributes to the different tumor growth rate and metastasis. In this study, we explored the distinct proteomic profiles between these two cancers and found different expression of glycogen phosphorylases in two cancers.

Methods
We explored novel targets by proteomics. Five CCRCC cases and five CHRCC cases were selected for tandem mass tag-labeling liquid chromatography-mass spectroscopy (LC–MS). Gene ontology and KEGG pathway were applied for bioinformatic analysis. Glycogen phosphorylases were detected by Western blotting.

Results
CHRCC were younger, more commonly female, and had larger tumors compared to those with CCRCC. 101 differentially expressed proteins (DEPs) in CCRCC and 235 DEPs in CHRCC were detected by LC–MS. It was found that disruption of metabolic pathways, epithelial cell differentiation, and cell response were the common characters for two tumor types. Activation of cell–cell adhesion and oxidation–reduction process stimulate CCRCC growth and epithelial cell differentiation and transferrin transport was involved in CHRCC growth, We also found that oxidative phosphorylation is activated in CHRCC and inhibited in CCRCC. More importantly, we found and confirmed that upregulation of glycogen phosphorylase liver type in CCRCC and glycogen phosphorylase brain type in CHRCC mediated differential glycogenolysis in the two tumor types, which could serve as potential therapeutic targets.

Conclusion
We found different expression of glycogen phosphorylases in CCRCC and CHRCC by quantitative proteomics, which provides potential therapeutic targets in the future.
Downregulation of hsa-microRNA-204-5p and identification of its potential regulatory network in non-small cell lung cancer: RT-qPCR, bioinformatic- and meta-analyses
Authors: Liang Chang-Yu , Li Zu-Yun , Gan Ting-Qing , Fang Ye-Ying , Gan Bin-Liang , Chen Wen-Jie , Dang Yi-Wu , Shi Ke , Feng Zhen-Bo , Chen Gang 
Published: February 2020

Abstract
Background
Pulmonary malignant neoplasms have a high worldwide morbidity and mortality, so the study of these malignancies using microRNAs (miRNAs) has attracted great interest and enthusiasm. The aim of this study was to determine the clinical effect of hsa-microRNA-204-5p (miR-204-5p) and its underlying molecular mechanisms in non-small cell lung cancer (NSCLC).

Methods
Expression of miR-204-5p was investigated by real-time quantitative PCR (RT-qPCR). After data mining from public online repositories, several integrative assessment methods, including receiver operating characteristic (ROC) curves, hazard ratios (HR) with 95% confidence intervals (95% CI), and comprehensive meta-analyses, were conducted to explore the expression and clinical utility of miR-204-5p. The potential objects regulated and controlled by miR-204-5p in the course of NSCLC were identified by estimated target prediction and analysis. The regulatory network of miR-204-5p, with its target genes and transcription factors (TFs), was structured from database evidence and literature references.

Results
The expression of miR-204-5p was downregulated in NSCLC, and the downtrend was related to gender, histological type, vascular invasion, tumor size, clinicopathologic grade and lymph node metastasis (P<0.05). MiR-204-5p was useful in prognosis, but was deemed unsuitable at present as an auxiliary diagnostic or prognostic risk factor for NSCLC due to the lack of statistical significance in meta-analyses and absence of large-scale investigations. Gene enrichment and annotation analyses identified miR-204-5p candidate targets that took part in various genetic activities and biological functions. The predicted TFs, like MAX, MYC, and RUNX1, interfered in regulatory networks involving miR-204-5p and its predicted hub genes, though a modulatory loop or axis of the miRNA-TF-gene that was out of range with shortage in database prediction, experimental proof and literature confirmation.

Conclusions
The frequently observed decrease in miR-204-5p was helpful for NSCLC diagnosis. The estimated target genes and TFs contributed to the anti-oncogene effects of miR-204-5p.
Exploration on sagittal alignment and clinical outcomes after consecutive three-level hybrid surgery and anterior cervical discectomy and fusion: a minimum of a 5-year follow-up
Authors: Xu Shuai , Liang Yan , Yu Guanjie , Zhu Zhenqi , Wang Kaifeng , Liu Haiying 
Published: February 2020

Abstract
Purpose
To compare sagittal alignment and clinical outcomes between three-level hybrid surgery (HS) and anterior cervical discectomy and fusion (ACDF) on cervical spondylotic myelopathy (CSM) over a 5-year follow-up.

Method
The study included 32 patients with ACDF, 36 patients with 1 prosthesis and 2 cages (HS1 group), and 25 cases with 2 prostheses and 1 cage (HS2 group). Alignment parameters included C2–C7 cervical lordosis (CL), C2–C7 sagittal vertical axis (SVA), T1 slope (T1S), and T1S minus CL (T1SCL). Radiographic parameters were range of motion (ROM), upper and lower adjacent ROM (UROM and LROM), and operated-segment lordosis (OPCL), as well as adjacent segment degeneration (ASD). Clinical outcomes included the neck disability index (NDI) and Japanese Orthopedic Association (JOA) score.

Results
Three groups were well-matched in demographics. All groups gained comparable improvement on NDI and JOA (P < 0.01). All groups gained CL improvement at the final visit (P < 0.05). There were no statistical differences on SVA and T1SCL among the groups and among preoperation, 1 week later, and final follow-up (P > 0.05) while T1S improved at 1 week later and final follow-up with HS2. The final change of all alignment parameters among the three groups was of no differences. ROM decreased and OPCL increased in all groups at the final follow-up (P < 0.05). UROM and LROM increased with ACDF but kept stable with HS1 and HS2. There was no inter-group difference on the incidence of ASD (P > 0.05).

Conclusion
Cervical alignment was comparably improved. HS and ACDF provided identified mid-term efficacy, and it was not necessary to have to use prosthesis on three-level CSM.
Male and LGBT survivors of sexual violence in conflict situations: a realist review of health interventions in low-and middle-income countries
Authors: Kiss Ligia , Quinlan-Davidson Meaghen , Pasquero Laura , Tejero Patricia Ollé , Hogg Charu , Theis Joachim , Park Andrew , Zimmerman Cathy , Hossain Mazeda 
Published: February 2020

AbstractConflict-related sexual violence (CRSV) against women and girls has been the subject of increasing research and scholarship. Less is known about the health of men, boys and lesbian, gay, bisexual, transgender (LGBT) and other gender non-binary persons who survive CRSV. This paper is the first systematic realist review on medical, mental health and psychosocial support (MHPSS) interventions that focusses on male and LGBT survivors of CRSV. The review explores the gender differences in context, mechanisms and outcomes that underpin interventions addressing the health and psychosocial wellbeing of male and LGBT survivors. The aim is to contribute to the design and delivery of gender-sensitive and, when needed, gender-specific approaches for interventions that respond to specific needs of different groups of all survivors. We conducted a systematic search of academic and grey literature to identify medical and MHPSS interventions that included men, boys and LGBT survivors. We identified interventions specifically targeting women and girls that we used as comparators. We then purposively sampled studies from the fields of gender and health, and sexual abuse against men and LGBT people for theory building and testing. We identified 26 evaluations of interventions for survivors of CRSV. Nine studies included male survivors, twelve studies focussed exclusively on female survivors and one study targeted children and adolescents. No intervention evaluation focussed on LGBT survivors of CRSV. The interventions that included male survivors did not describe specific components for this population. Results of intervention evaluations that included male survivors were not disaggregated by gender, and some studies did not report the gender composition. Although some mental health and psychosocial consequences of sexual violence against men and boys may be similar among male and female survivors, the way each process trauma, display symptoms, seek help, adhere to treatment and improve their mental health differ by gender. Initiatives targeting male and LGBT survivors of CRSV need to be designed to actively address specific gender differences in access, adherence and response to MHPSS interventions. Models of care that are gender-sensitive and integrated to local resources are promising avenues to promote the health of male and LGBT survivors of CRSV.
Effects of moving cupping therapy for plaque psoriasis: study protocol for a randomized multicenter clinical trial
Authors: Xing Meng , Yan Xiaoning , Yang Suqing , Li Linge , Gong Liping , Liu Hongxia , Xu Rong , Chen Jie , Ying Luo , Zhao Yiding , An Yuepeng , Liu Yang , Huang Gang , Guo Fei , Yin Qingfeng , Wang Ruiping , Li Bin , Li Xin 
Published: February 2020

Abstract
Background
It is difficult to achieve a balance among safety, efficacy, and cost for the clinical treatment of plaque psoriasis. The current treatment of psoriasis often involves comprehensive therapy such as topical plasters, internal medicine, and phototherapy, which are expensive, and some of the drugs have serious side effects. Moving cupping is a type of cupping that has been used clinically for thousands of years in China. It has the advantage of being inexpensive and easy to perform. Therefore, it is widely used in public hospitals in China for psoriasis treatment. However, a comprehensive evaluation of the current clinical evidence of its efficacy is lacking. In this study, we aimed to evaluate the efficacy and safety of moving cupping to treat plaque psoriasis.

Methods
A multicenter, two-arm parallel group, single-blind, randomized, controlled trial will be conducted at six hospitals in China between August 1, 2019 and December 31, 2021. A total of 122 adult patients (aged 18–65 years) who meet the inclusion criteria are being recruited. Participants will receive either basic treatment combined with moving cupping therapy or basic treatment combined with moving cupping placebo. The treatment cycle will be 4 weeks, and the efficacy of treatment will be assessed weekly by the Psoriasis Area and Severity Index during the treatment period and follow-up visits at weeks 6 and 8. The body surface area, physician’s global assessment, Dermatology Life Quality Index, patient-reported quality of life, visual analog scale, Traditional Chinese Medication syndrome scoring scale, combined medication, and adverse events will also be recorded and compared to the relative baseline values.

Discussion
The findings of this trial may lead to better decisions regarding the treatment of plaque psoriasis. If the trial outcomes are considered favorable, this ancient Chinese medical therapy may be worthy of widespread use because of its convenience and low cost.

Trial registration
This study was registered on May 15,2019 at ClinicalTrials.gov with the identifier number NCT03952676.
Protein-based biorefining driven by nitrogen-responsive transcriptional machinery
Authors: Ma Lianjie , Guo Liwei , Yang Yunpeng , Guo Kai , Yan Yajun , Ma Xiaoyan , Huo Yi-Xin 
Published: February 2020

Abstract
Background
Protein-based bioconversion has been demonstrated as a sustainable approach to produce higher alcohols and ammonia fertilizers. However, owing to the switchover from transcription mediated by the bacterial RNA polymerase σ70 to that mediated by alternative σ factors, the biofuel production driven by σ70-dependent promoters declines rapidly once cells enter the stationary phase or encounter stresses. To enhance biofuel production, in this study the growth phase-independent and nitrogen-responsive transcriptional machinery mediated by the σ54 is exploited to drive robust protein-to-fuel conversion.

Results
We demonstrated that disrupting the Escherichia coli ammonia assimilation pathways driven by glutamate dehydrogenase and glutamine synthetase could sustain the activity of σ54-mediated transcription under ammonia-accumulating conditions. In addition, two σ54-dependent promoters, argTp and glnAp2, were identified as suitable candidates for driving pathway expression. Using these promoters, biofuel production from proteins was shown to persist to the stationary phase, with the net production in the stationary phase being 1.7-fold higher than that derived from the optimal reported σ70-dependent promoter PLlacO1. Biofuel production reaching levels 1.3- to 3.4-fold higher than those of the σ70-dependent promoters was also achieved by argTp and glnAp2 under stressed conditions. Moreover, the σ54-dependent promoters realized more rapid and stable production than that of σ70-dependent promoters during fed-batch fermentation, producing up to 4.78 g L − 1 of total biofuels.

Conclusions
These results suggested that the nitrogen-responsive transcriptional machinery offers the potential to decouple production from growth, highlighting this system as a novel candidate to realize growth phase-independent and stress-resistant biofuel production.
Prognosis after autologous peripheral blood stem cell transplantation for osteonecrosis of the femoral head in the pre-collapse stage: a retrospective cohort study
Authors: Pan Jiafei , Ding Quanwei , Lv Shuaijie , Xia Bingjiang , Jin Hongting , Chen Di , Xiao Luwei , Tong Peijian 
Published: February 2020

Abstract
Objectives
Autologous peripheral blood stem cell (auto-PBSC) transplantation is an effective therapeutic for the osteonecrosis of the femoral head (ONFH) but without prognosis estimation. This study mainly aimed to (1) determine whether auto-PBSC transplantation is a promising option, (2) assess the risk of hip-preservation failure, (3) achieve a predictive model of femoral head survival after the intervention, and (4) eventually identify clinical indications for auto-PBSC transplantation in future.

Methods
After reviewing the in-patient database of the First Affiliated Hospital of Zhejiang Chinese Medicine University from June 2012 to June 2014, 37 eligible patients with Association Research Circulation Osseous stage I or II ONFH who were receiving intra-arterial infusion of auto-PBSCs were recruited. A case form was designed to retrieve relevant data. Hip-preservation failure was defined as the endpoint. All participants were stratified by the categorical risk of collapse, which was statistically tested through log-rank analysis. All significant factors were evaluated using Cox proportional hazards regression model, and a predictive nomogram plot was generated.

Results
In total, 47 hips were followed up for 53.96 ± 21.09 months; the median survival time was 60.18 months. Among the predictors, body mass index (BMI; P = 0.0015) and Harris hip score (HHS; P < 0.0001) independently affected femoral head survival. Patients with BMI ≥ 24 kg/m2 exhibited a 2.58 times higher risk of hip-preservation failure [95% confidence interval (CI), 1.32–5.45] than those with BMI < 24 kg/m2, whereas those with HHS ≥ 70 exhibited a 0.19 times lower risk (95% CI, 0.09–0.38) than those with HHS < 70. Hazard ratios associated with age (P = 0.042), BMI (P = 0.012), HHS (P = 0.022), and necrotic volume (P = 0.000) were 1.038 (95% CI, 1.001–1.075), 1.379 (95% CI, 1.072–1.773), 0.961 (95% CI, 0.928–0.994), and 1.258 (95% CI, 1.120–1.412), respectively. A nomogram plot (score test P = 0.000; C-index = 0.8863) was available for the orthopedic doctor to predict hip survival probability.

Conclusions
The results suggest that intra-arterial infusion of auto-PBSCs prolongs femoral head survival. Age, BMI, HHS, and necrotic volume can influence the efficacy of this intervention.
This study was approved by ethics committee of the trial center, number 2019-KL-075-01.
Global existence and exponential decay of strong solutions for the three-dimensional Boussinesq equations
Authors: Shang Zhaoyang , Tang Fuquan 
Published: February 2020

AbstractIn this paper, we consider the global existence of strong solutions to the three-dimensional Boussinesq equations on the smooth bounded domain Ω. Based on the blow-up criterion and uniform estimates, we prove that the strong solution exists globally in time if the initial $L^{2}$L2-norm of velocity and temperature are small. Moreover, an exponential decay rate of the strong solution is obtained.
Sustainability evaluation of community-based, solar photovoltaic projects in Malawi
Authors: Dauenhauer Peter M. , Frame Damien , Eales Aran , Strachan Scott , Galloway Stuart , Buckland Hannah 
Published: February 2020

Abstract
Background
A novel project sustainability framework is used to evaluate 65 off-grid solar photovoltaic (PV) energy system projects in Malawi. This study addresses PV projects serving rural public facilities, a solution known to have had historical issues with poor sustainability. A recent countrywide program targeting such facilities was evaluated against existing projects to determine whether this latest iteration offered better results.

Method
Sustainability is defined at the project-level with metrics justified under the main themes of technical, economic, social, and organizational. Data captured for each project is based on a custom survey and interview of key stakeholders. Projects are grouped according to age, project implementer, income level, and PV system size to compare relative results.

Results
The sustainability outlook for each project is evaluated. We find sustainability for most projects to be low. Social sustainability was weak with many projects due to low local community stakeholder engagement in terms of numbers of stakeholders, meeting frequency, and community contributions. Organizational challenges included a lack of key management positions in place and extremely limited training provisions. Furthermore, the evaluation highlights the economic health of the system to be key to sustainability, with the “healthiest” projects affording only 37% of expected operations and maintenance costs. Relative to expected demand, systems were found to be undersized for both panels (53% of required) and batteries (57%). Users reported achieving only 60% of their desired consumption. Poor sizing standards related to the lack of load profiles for first-time electricity users as well as poor quality components reduced the overall technical sustainability.

Conclusion
Rural public facilities with solar PV in Malawi are not well served by isolated community management. Improved sustainability requires the establishment of a robust financial framework prior to project development that includes formal local government support. This paper discusses ideal management frameworks and their ultimate implications for project developers, policy makers, and the research community.