bluuebunny/crossref_metadata_2025_split huggingface dataset:
Role of Interprofessional Primary Care Teams in Preventing Avoidable Hospitalizations and Hospital Readmissions in Ontario, Canada: A Retrospective Cohort Study
Authors: Haj-Ali Wissam , Moineddin Rahim , Hutchison Brian , Wodchis Walter P , Glazier Richard H. 
Published: August 2020

Abstract
Background: Improving health system value and efficiency are considered major policy priorities internationally. Ontario has undergone a primary care reform that included introduction of interprofessional teams. The purpose of this study was to investigate the relationship between receiving care from interprofessional versus non-interprofessional primary care teams and ambulatory care sensitive condition (ACSC) hospitalizations and hospital readmissions. Methods: Population-based administrative databases were linked to form data extractions of interest between the years of 2003-2005 and 2015-2017 in Ontario, Canada. The data sources were available through ICES. The Study Design was a Retrospective longitudinal cohort. We used a “difference-in-differences” approach for evaluating changes in ACSC hospitalizations and hospital readmissions before and after the introduction of interprofessional team-based primary care while adjusting for physician group, physician and patient characteristics. Principal Findings: As of March 31st, 2017, there were a total of 778 physician groups, of which 465 were blended capitation Family Health Organization (FHOs); 177 FHOs (22.8%) were also interprofessional teams and 288 (37%) were more conventional group practices (“non-interprofessional teams”). In this period, there were a total of 13,480 primary care physicians in Ontario of whom 4,848 (36%) were affiliated with FHOs—2,311 (17.1%) practicing in interprofessional teams and 2,537 (18.8%) practicing in non-interprofessional teams. During that same period, there were 475,611 and 618,363 multi-morbid patients in interprofessional teams and non-interprofessional teams respectively out of a total of 2,920,990 multi-morbid adult patients in Ontario. There was no difference in change over time in ACSC admissions between interprofessional and non-interprofessional teams between the pre- and post intervention periods. There were no statistically significant changes in all cause hospital readmission s between the post- and pre-intervention periods for interprofessional and non-interprofessional teams. Conclusion: Our study findings indicate that the introduction of interprofessional team-based primary care was not associated with changes in ACSC hospitalization or hospital readmissions. The findings point for the need to couple interprofessional team-based care with other enablers of a strong primary care system to improve health services utilization efficiency.
Griseococcin(1) from Bovistella radicata (Mont.) Pat and antifungal activity
Authors: ye yong , Zeng Qinghua , zeng qingmei 
Published: August 2020

Abstract
Background To evaluate the antimicrobial and microbicidel activity of B. radicata fermentation broth, the broth was purified by DEAE-cellulose and sephadex LC-20 column. The compounds were submitted to spectral analyses (HPLC, FT-IR, 1D and 2D NMR etc.). Results The purified compounds were identified as the Griseococcin(s) which were naphthoquinone derivatives, the Chemical formula and MW of Griseococcin(1) was determined as C37O10H43N and 661Da. only Griseococcin(1) has good antimicrobial activity among the Griseococcin(s). The zone of inhibition(ZOI), minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) or minimum fungicidal concentration (MFC) of Griseococcin(1) were used to investigate the antimicrobial activity. Antifungal activity of Griseococcin(1) was significant, especially for main pathogenic fungus Trichophyton rubrum and Trichophyton mentagrophytes, MFC/MIC of Griseococcin(1) was 1, while MFC/MIC of postive control was greater than 4, the fungicidal effect of Griseococcin(1) was better than that of positive control.Conclusions In this paper, the secondary metabolite compound Griseococcin(1) from B. radicata was purified. The purified compound can restrain main pathogens(T. rubrum and T. mentagrophytes) leading to tinea pedis. The antifungal activity of Griseococcin(1) was similar to that of the positive control and the fungicidal effect of Griseococcin(1) was better than that of positive control, it might be suitable for pharmaceutical industries.
Optimization Strategy of Wireless Charger Node Deployment Based on Improved Cuckoo Search Algorithm
Authors: Wang Yang , wang feifan , Zhu Yujun , Liu Yiyang , Zhao Chuanxin 
Published: August 2020

Abstract
In wireless rechargeable sensor network, the deployment of charger node directly affects the overall charging utility of sensor network. Aiming at this problem, this paper abstracts the charger deployment problem as a multi-objective optimization problem that maximizes the received power of sensor nodes and minimizes the number of charger nodes. First, a network model that maximizes the sensor node received power and minimizes the number of charger nodes is constructed. Second, an Improved Cuckoo Search (ICS) algorithm is proposed. This algorithm is based on the traditional Cuckoo Search algorithm (CS) to redefine its step factor, and then use the mutation factor to change the nesting position of the host bird to update the bird ’s nest position, and then use ICS to find the ones that maximize the received power of the sensor node and minimize the number of charger nodes optimal solution. Compared with the traditional cuckoo search algorithm and multi-objective particle swarm optimization algorithm, the simulation results show that the algorithm can effectively increase the receiving power of sensor nodes, reduce the number of charger nodes and find the optimal solution to meet the conditions, so as to maximize the network charging utility.
Comparative evaluation of two commercial real-time PCR kits (QuantiFast™ and abTES™) for the detection of Plasmodium knowlesi and other Plasmodium species in Sabah, Malaysia
Authors: Nuin Nor Afizah , Tan Angelica Fiona , Lew Yao Long , Piera Kim A , William Timothy , Rajahram Giri Shan , Jelip Jenarun , Dony Jiloris F , Mohammad Rashidah , Cooper Daniel J , Barber Bridget E , Anstey Nicholas M , Chua Tock H , Grigg Matthew J 
Published: August 2020

Abstract
Introduction: The monkey parasite Plasmodium knowlesi is an emerging public health issue in Southeast Asia. In Sabah, Malaysia, P. knowlesi is now the dominant cause of human malaria. Molecular detection methods for P. knowlesi are essential for accurate diagnosis and in monitoring progress towards malaria elimination of other Plasmodium species. However, recent commercially available PCR malaria kits have unpublished P. knowlesi gene targets or have not been evaluated against clinical samples. Methods: Two real-time PCR methods currently used in Sabah for confirmatory malaria diagnosis and surveillance reporting were evaluated: the QuantiFast™ Multiplex PCR kit (Qiagen, Germany) targeting the P. knowlesi 18S SSU rRNA; and the abTES™ Malaria 5 qPCR II kit (AITbiotech, Singapore), with an undisclosed P. knowlesi gene target. Diagnostic accuracy was evaluated using 52 P. knowlesi, 25 P. vivax, 21 P. falciparum, and 10 P. malariae clinical isolates, and 26 malaria negative controls, and compared against a validated reference nested PCR assay. The limit of detection (LOD) for each PCR method and Plasmodium species was also evaluated. Results: The sensitivity of the QuantiFast™ and abTES™ assays for detecting P. knowlesi was comparable at 98.1% (95%CI 89.7-100) and 100% (95%CI 93.2-100) respectively. Specificity of the QuantiFast™ and abTES™ for P. knowlesi was high at 98.8% (95%CI 93.4-100) for both assays. The QuantiFast™ assay demonstrated falsely-positive mixed Plasmodium species at low parasitaemias in both the primary and LOD analysis. Diagnostic accuracy of both PCR kits for detecting P. vivax, P. falciparum, and P. malariae was comparable to P. knowlesi. The abTES™ assay demonstrated a lower LOD for P. knowlesi of ≤0.125 parasites/µL compared to QuantiFast™ with a LOD of 20 parasites/µL. Hospital microscopy demonstrated a sensitivity of 78.8% (95%CI 65.3-88.9) and specificity of 80.4% (95%CI 67.6-89.8) compared to reference PCR for detecting P. knowlesi. Conclusion: The QuantiFast™ and abTES™ commercial PCR kits performed well for the accurate detection of P. knowlesi infections. Although the QuantiFast™ kit is cheaper, the abTES™ kit demonstrated a lower LOD, supporting its use as a second-line referral-laboratory diagnostic tool in Sabah, Malaysia.
Gender Difference Analysis of Xp11.2 Translocation Renal Cell Carcinomas’s Attack Rate: a meta-analysis and systematic review
Authors: Zhuang Wenyuan , Liu Ning , Guo Hongqian , Gan Weidong , Zhang Chunni 
Published: August 2020

Abstract
Background: Xp11.2 translocation renal cell carcinoma (tRCC) is recently recognized. As Xp11.2 tRCC involved gene translocation and fusion in X chromosome and the number of X chromosomes in female is twice of male, we wondered whether the gender difference of attack rate is consistent with the proportion of the X chromosome. Methods: In the present paper, meta-analysis was performed to find out the difference of morbidity between male and female. Results: 9 studies with 209 cases calculated. Odds ratios (ORs) and ORs with 95% confidence intervals (CIs) were calculated for attack rate of Xp11.2 RCC with different gender. The result showed that the attack rate of female was higher than that of male with pooled OR of 2.84 (95 % CI = 1.48–5.45), while the rate rises even further in adult (OR =3.37, 95 % CI =2.19–5.18). In other types of common kidney cancer, the OR value is less than 1, which means that the incidence of female is lower than that of male.Conclusions: The result showed that the incidence rate of female patients is much higher than that of male patients with Xp11.2 tRCC, it was reasonable to indicate that this particular incidence rate is related to the X chromosome.
Effectiveness Of Structured Training Programme Regarding Adult Basic Life Support On Knowledge Of Nurses` In Zoba Maekel And National Referral Hospitals, Eritrea, 2018.
Authors: Ghirmai Filmon Abraham , Weldeghiorgis Ghidey Ghebreyowhanness , Bahlebi Teklu Tsegay , Tesfamariam Eyasu Habte 
Published: August 2020

Abstract
Background: In Eritrea nurses provide most of the health care in the primary, secondary and tertiary level health facilities. The rate of trauma and medical emergencies are on the increase so nurses should have adequate and updated knowledge on Basic Life Support in order to be able to implement effective interventions in cardiac arrest situations. Aim: The objective of this study was to assess the effectiveness of structured training programme regarding Adult Basic Life Support on knowledge of nurses.Method: A quasi-experimental single group, pretest-posttest and retest design was utilized. Forty (40) nurses’ participated in the study. Structured and modified questionnaire was used to collect data.Data were analyzed using the Statistical Package for Social Sciences (SPSS) Version 22.0 program. Frequencies, percentages, mean and standard deviation was used to describe the data. One way repeated measures of ANOVA was used to check the possible change in knowledge of the nurses at pre- intervention, immediate and three months’ after intervention. A p-value < 0.05 was considered to be statistically significant.Results: The findings showed an acquisition of Adult Basic Life Support knowledge among the study participants following a four hour Basic Life Support training programme. Before training, the mean score of the study participant on knowledge questions was 9.58/20 and none of the respondents got a pass mark. Immediately after the training the mean score of the study participants was increased to 15.9/20 and 55.0% of the study participant got a passing mark. Significant deterioration from the immediate training score was found three months following Basic Life Support training as score was decreased to 14.9 and 40% of the respondents got a pass mark. Nurses’ knowledge was improved from their pre-training scores, which clearly indicated a positive retention in Basic Life Support knowledge after training. Conclusions: The study findings present strong evidence that support the critical role of structured training programme about Adult Basic Life Support in improving nurses knowledge.
A Feasibility Study Of A Cognitive Behavioral Based Stress Management Intervention For Nursing Students: Results, Challenges, And Implications For Research And Practice
Authors: Terp Ulrik , Bisholt Birgitta , Hjärthag Fredrik 
Published: August 2020

Abstract
Background: Stress related psychological problems are growing in nursing education and constitute an essential challenge for educators. This makes research about strategies and interventions to meet these problems important. Before implementing stress management interventions, they need to be tested for feasibility and acceptability before conducting large scale RCTs. The objective of our study was to assess the feasibility and acceptability of a newly developed cognitive behavioral stress management intervention for nursing students. Methods: Data were collected using a combination of standardized measurements and newly created questionnaires in combination with qualitative data. Our data included recruitment capability, sample characteristics, intervention acceptability and preliminary evaluation of participant psychological changes. Results: Findings suggested that the feasibility of conducting a full-scale evaluation was confirmed for recruitment, acceptability, data collection, and adherence to the intervention. However, difficulties relating to homework were also identified. The intervention was found feasible and acceptable to nursing students and a potential intervention for the nurse education context. Conclusions: Overall, this study provides an insight into the challenges and complexities of developing and evaluating a new brief cognitive behavioral based stress management training intervention in a nurse education setting. Support in the organization and scheduling are important aspects of implementation. In order to deal with the challenge of stress-related problems in nursing education, the thresholds for participation in effective interventions need to be low.
Ultrasensitive gene fusion detection reveals fusion variant associated tumor heterogeneity
Authors: Zhang Baifeng , Song Zhengbo , Bao Chloe Yufan , Xu Chunwei , Wang Wenxian , Chu Hoi Yee , Lu Chenyu , Wang Hongxiang , Bao Siyu , Gong Zhenyu , Keung Hoi Yee , Chow Maggie , Zhang Yiping , Cheuk Wah , Yang Mengsu , Cho William , Chen Juxiang , Zheng Zongli 
Published: August 2020

Abstract
Gene fusions are common drivers and therapeutic targets in cancers, but clinical-grade bioinformatics callers are lacking. Here we introduce a novel method SplitFusion, which is fast by leveraging BWA-MEM split alignments, can detect cryptic splice site fusions, and can infer frame-ness and exon-boundary alignments for functional prediction and minimizing false-positives. SplitFusion demonstrates superior sensitivity, specificity, accuracy and consumes minimal computing resources. In our study of 1,076 formalin-fixed paraffin-embedded lung cancer samples, SplitFusion detected not only common fusions (EML4 4.7%, ROS1 2.0% and RET 1.1%) with various partners, but also rare (KLC1-ALK, CD74-NRG1, and TPR-NTRK1) and novel (FGFR3-JAKMP1, CLIP2-BRAF, and ITPR2-ETV6) fusions. In 35 glioblastoma samples, SplitFusion-Target detected six (17%) EGFR vIII (exons 2-7 deletion) cases. Furthermore, we find that the EML4-ALK variant 3 is significantly associated with occurrence of multiple breakpoint-defined subclones, namely high intratumor heterogeneity. In conclusion, SplitFusion is well-suited for clinical use and for studying fusion-defined tumor heterogeneity.
Adjuvant SOX Chemotherapy Versus Concurrent Chemoradiotherapy After D2 Radical Resection of Locally Advanced Esophagogastric Junction (EGJ) Adenocarcinoma: Study Protocol for a Randomized Phase III Trial (ARTEG)
Authors: Shen Jinwen , Zhu Xiu , Du Yian , Zhu Yuan , Yu Pengfei , Yang Litao , Xu Zhiyuan , Huang Ling , Zhang Yunli , Zhang Yanqiang , Liu Luying , Cheng Xiangdong 
Published: August 2020

Abstract
Background: Survival benefit of adjuvant radiotherapy for locally advanced gastric cancer following gastrectomy plus D2 lymphadenectomy has always been controversial. Esophagogastric junction (EGJ) adenocarcinoma, which is usually classified as gastric cancer in East Asia, often has a higher locoregional recurrence rate after operation because of its special anatomical characteristics. The aim of this study is to determine whether adjuvant radiotherapy can improve survival of locally advanced EGJ adenocarcinoma after D2 radical resection.Methods: In this phase III, randomized, open label, controlled trial, we plan to recruit 378 patients with Siewert type II and III adenocarcinoma of EGJ, who had undergone transabdominal radical surgery and D2 lymphadenectomy, and were divided into pathological stage IIB to IIIC. All patients will be randomized 1:1 to receive either adjuvant chemotherapy alone (control group) or adjuvant chemotherapy plus chemoradiotherapy (experimental group). Patients allocated to control group will receive eight cycles of S-1 plus oxaliplatin (SOX), while experimental group will receive two cycles of SOX followed by 45-Gy RT combined with S-1 and four additional cycles of SOX. The primary endpoint is three-year disease free survival rate (DFS). The secondary endpoints are three-year overall survival rate (OS), three-year locoregional recurrence free survival rate (LRFS), three-year distant metastasis free survival rate (DMFS), and quality of life (QoL).Discussion: In the past, the adjuvant treatment of EGJ adenocarcinoma need to draw on the experience of esophageal adenocarcinoma or gastric adenocarcinoma. In this study, EGJ adenocarcinoma is considered as an independent disease, and the conclusion will provide evidence for optimal adjuvant therapy of locally advanced EGJ adenocarcinoma after D2 radical resection.Trial registration: ClinicalTrials.gov, NCT03973008. Registered on June 1, 2019.
Effects of NAT2 Polymorphism on Lung Cancer Risk, and the Interaction Between Smoking and NAT2: A Meta-analysis
Authors: Zhu Ke , Xu Aiqun , Zhang Binbin , Li Pulin , Jiang Huihui , Zhou Sijing , Wang Ran 
Published: August 2020

Abstract
Background

Lung cancer is the most common cancer in the world, and cancer death is mainly caused by lung cancer. At present, the etiology and pathogenesis of lung cancer are not clear. N-acetyltransferase 2 (NAT2) is an enzyme found in the lungs, colon, breast, prostate, and liver. NAT2 is polymorphic and can metabolize carcinogens from tobacco smoke. At present, many studies have explored the effects of NAT2 polymorphism on lung cancer, but we found inconsistent results.
Methods

We conducted a research of 19 published studies, involving 4,130 patients and 6,057 controls, to more accurately assess the effects of NAT2 polymorphism on lung cancer risk and to investigate whether smoking is associated. We used STATA software to analyze the extracted data and used STATA for subgroup analysis, sensitivity analysis, and to perform publication bias tests. To determine the correlation, we used the crude odds ratio (ORs) with 95% confidence interval (CIs).
Results

From the major results, we found that there was no significant correlation between NAT2 polymorphism and lung cancer (OR = 1.00, 95% CI: 0.84–1.19, I² = 63.3%, P < 0.001 for heterogeneity). We also found no significant results in stratified analyses of smoking, ethnicity, gender, and source of controls. However, we learned from the subgroup analysis that the lung cancer risk in NAT2 patients with intermediate-slow acetylation may be increased (OR = 1.83, 95% CI: 1.22–2.73, I² = 39.6%, P = 0.191 for heterogeneity).
Conclusions

This research showed that no sufficient evidence was found to prove the effect of NAT2 polymorphism on lung cancer risk; however the increased acetylation capacity of NAT2 might reduce the risk of lung cancer.
Social and Economic Consequences of the Cost of Obstetric and Neonatal Care in Lubumbashi, in the Democratic Republic of Congo: Mixed Study
Authors: MUSAU Angèle NKOLA , Ntambue Abel Mukengeshayi , Omewatu Jacques Mungomba , Mundongo Henri Tshamba , Malonga Françoise Kaj 
Published: August 2020

Abstract
Background: The aim of this study was to explore and measure the social and economic consequences of the cost of obstetric and neonatal care in Lubumbashi, the Democratic Republic of Congo.Methods: We conducted a mixed qualitative and quantitative study in the maternity departments of health facilities in Lubumbashi. The qualitative results were based on a case study conducted in 2018 that included 14 respondents (8 parturients, 2 accompanying family members and 4 health care providers). A quantitative cross-sectional analytical study was carried out in 2019 with 411 women who gave birth at 10 referral hospitals. Data were collected for one month at each hospital, and selected parturients were included in the study only if they paid out-of-pocket and at the point of care for costs related to obstetric and neonatal care.Results: Costs for obstetric and neonatal care averaged US $77, US $207 and US $338 for simple, complicated vaginal and cesarean deliveries, respectively. These health expenditures were greater than or equal to 40% of the ability to pay for 58.4% of households. At the time of delivery, 14.1% of women in childbirth did not have enough money to pay for care. Of those who did, 76.5% spent their savings. When households did not pay for care, mothers and their babies were held for a long time at the place of care. This resulted in prolonged absence of the mother from the household, reduced household income, family conflicts, and the abandonment of the home by the spouse. At the health facility level, the length of stay increased without generating any additional financial benefits. Disrespectful care and the deterioration of the relationships between caregivers and parturients were also recorded.Conclusion: To reduce the social and economic consequences of care, the government of the DRC should implement a mechanism for subsidizing care and should associate it with a cost-sharing system. This would result in achieving universal health coverage and improving the physical, mental and social health of mothers, their babies and their households.
LncRNA LEF1-AS1 promotes metastasis of prostatic carcinoma via the Wnt/β-catenin pathway
Authors: Li Weiyuan , Yang Ganggang , Yang Dengke , Li Dong , Sun Qian 
Published: August 2020

Abstract
Background: Long noncoding RNAs (lncRNAs), which are important functional regulators in cancer, have emerged as critical molecular regulators in various biological processes. However, the mechanisms by which LEF1-AS1 modulates Androgen-Independent Prostate Cancer (AIPC) development remain largely unknown.Methods: The LEF1-AS1 expression level was detected in tumour tissues and adjacent normal tissues of AIPC patients by using next-generation sequencing technology and qRT-PCR. Cell proliferation, migration and invasion were assessed by colony formation, EDU assays and transwell assays, respectively. Xenograft assay was conducted to determine the effect of LEF1-AS1 on cell proliferation in vivo.Results: LEF1-AS1 promoted the proliferation, migration, invasion and angiogenic ability of AIPC cells in vitro and in vivo. In this mechanism, LEF1-AS1 recruited the transcription factor C-myb to the promoter region of FZD2, which activated FZD2 transcription. Moreover, LEF1-AS1 functioned as a competing endogenous RNA (ceRNA) acting as a sponge for miR-328, which activated CD44.Conclusion: Collectively, these data indicate that LEF1-AS1 is a tumour promoter in the development of AIPC and that it may contribute to the improvement of AIPC diagnosis and therapy.