bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of ticagrelor monotherapy on mortality after percutaneous coronary intervention: a systematic review and meta-analysis of randomized trials including 26 143 patients
Authors: Hong Sung-Jin , Ahn Chul-Min , Kim Jung-Sun , Kim Byeong-Keuk , Ko Young-Guk , Choi Donghoon , Jang Yangsoo , Hong Myeong-Ki 
Published: September 2020

Abstract

Aims
Optimal timing and strategy of antiplatelet monotherapy after dual-antiplatelet therapy (DAPT) consisting of aspirin and P2Y12 inhibitor for patients who underwent percutaneous coronary intervention (PCI) is still being debated. The aim of this study was to evaluate the effect of ticagrelor monotherapy after short-term DAPT after PCI on mortality.

Methods and results
A systematic review and meta-analysis was performed using PubMed to search for ticagrelor monotherapy after short-term DAPT comparing conventional DAPT in patients who underwent PCI. Three randomized trials encompassing 26 143 patients [ticagrelor monotherapy after 1–3 months of DAPT (n = 13 062) vs. conventional therapy (n = 13 081)] were included. The efficacy endpoint of all-cause mortality was significantly lower with the ticagrelor monotherapy group vs. the conventional therapy group [risk ratio (RR) = 0.80, 95% confidence interval (CI) 0.65–0.98; P = 0.03; I2 = 0%; number needed to treat for benefit (NNTB) = 320]. The safety endpoint of Bleeding Academic Research Consortium (BARC) type 3 or 5 bleeding was also significantly lower with the ticagrelor monotherapy group vs. the conventional therapy group (RR = 0.67, 95% CI 0.49–0.92; P = 0.01; I2 = 65%; NNTB = 156). There were no significant differences in ischaemic stroke, acute myocardial infarction, and stent thrombosis. The favourable effects of the ticagrelor monotherapy vs. the conventional therapy on all-cause mortality and BARC type 3 or 5 bleeding were consistent in the subset of patients presenting acute coronary syndromes (n = 15 157).

Conclusion
Ticagrelor monotherapy after short-term DAPT of 1–3 months was associated with decreased all-cause mortality and BARC type 3 or 5 bleeding not offset by increase of cardiac death, ischaemic stroke, acute myocardial infarction, and stent thrombosis.
Increased receptor expression supports vascular reactivity of the rabbit aorta during preservation
Authors: Cupitra Nelson Ivan , Calderón Juan C , Narvaez-Sanchez Raul 
Published: September 2020

Abstract

OBJECTIVES
The mechanistic understanding of vascular functional impairment during preservation time helps determine the optimal time frame in which explanted arteries can be used. The method of choice is to measure vascular reactivity and receptor expression. Our goal was to study the influence of preservation for 24 and 48 h on vascular reactivity and receptor expression in rabbit aorta.

METHODS
Aortic rings preserved in Krebs–Henseleit solution were evaluated fresh (t0), 24 h (t24) and 48 h (t48) after harvest for (i) vascular reactivity as sensitivity (pD2) and maximum effect in response to potassium chloride, U46619 (thromboxane-A2 agonist), phenylephrine, carbachol and isoproterenol, in an organ bath; and for (ii) expression of α1, β2 and thromboxane-prostanoid receptors, by immunofluorescence.

RESULTS
Compared to the control, after 24 h of preservation, potassium chloride-induced pD2 increased a significant 3.6%, whereas U46619-induced vasoconstriction decreased 9%. None of the agonists affected vasodilation. Intimal and medial α1 receptor expression increased 2.5-fold. After 48 h of preservation, α1 expression and vasoconstrictor responses remained similar to those after 24 h of preservation, but in vasodilation the carbachol-induced maximum effect decreased 30% whereas isoproterenol-induced pD2 increased 4% and the maximum effect increased 10%. TP and β2 expression in the intima and media increased 1.8- and 2.5-fold, respectively.

CONCLUSIONS
Up to 48 h of preservation, the adrenergic pathway and its receptors support vasoconstriction and vasodilation, despite a significant deterioration in the prostanoid pathway.
Shifts in the protist community associated with an anticyclonic gyre in the Alboran Sea (Mediterranean Sea)
Authors: García-Gómez Candela , Yebra Lidia , Cortés Dolores , Sánchez Antonio , Alonso Aitor , Valcárcel-Pérez Nerea , Gómez-Jakobsen Francisco , Herrera Inma , Johnstone Carolina , Mercado Jesús M 
Published: September 2020

ABSTRACT
The diversity of protists was researched in the Alboran Sea (SW Mediterranean Sea) by means of high-throughput sequencing technologies based on the amplification of the V9 region of 18S rRNA. Samples were collected at different depths in seven stations following an environmental gradient from a coastal upwelling zone to the core of an oligotrophic anticyclonic gyre (AG). Sampling was performed during summer, when the water column was stratified. The superphyla Alveolata, Stramenopila and Rhizaria accounted for 84% of the total operational taxonomic units (OTUs). The most diverse groups were Dinophyceae (21% of OTUs), Marine Alveolates-II (MALV-II; 20%), Ciliophora (9%) and MALV-I (6%). In terms of read abundance, the predominant groups were Dinophyceae (29%), Bacillariophyta (14%), MALV-II (11%) and Ciliophora (11%). Samples were clustered into three groups according to the sampling depth and position. The shallow community in coastal stations presented distinguishable patterns of diatoms and ciliates compared with AG stations. These results indicate that there was a strong horizontal coupling between phytoplankton and ciliate communities. Abundance of Radiolaria and Syndiniales increased with depth. Our analyses demonstrate that the stratification disruption produced by the AG caused shifts in the trophic ecology of the plankton assemblages inducing a transition from bottom-up to top-down control.
Aerobic microbial communities in the sediments of a marine oxygen minimum zone
Authors: Bhattacharya Sabyasachi , Roy Chayan , Mandal Subhrangshu , Sarkar Jagannath , Rameez Moidu Jameela , Mondal Nibendu , Mapder Tarunendu , Chatterjee Sumit , Pyne Prosenjit , Alam Masrure , Haldar Prabir Kumar , Roy Rimi , Fernandes Svetlana , Peketi Aditya , Chakraborty Ranadhir , Mazumdar Aninda , Ghosh Wriddhiman 
Published: September 2020

ABSTRACTThe ecology of aerobic microorganisms is never explored in marine oxygen minimum zone (OMZ) sediments. Here we reveal aerobic bacterial communities along ∼3 m sediment-horizons of the eastern Arabian Sea OMZ. Sulfide-containing sediment-cores retrieved from 530 mbsl (meters beneath the sea-level) and 580 mbsl were explored at 15–30 cm intervals, using metagenomics, pure-culture-isolation, genomics and metatranscriptomics. Genes for aerobic respiration, and oxidation of methane/ammonia/alcohols/thiosulfate/sulfite/organosulfur-compounds, were detected in the metagenomes from all 25 sediment-samples explored. Most probable numbers for aerobic chemolithoautotrophs and chemoorganoheterotrophs at individual sample-sites were up to 1.1 × 107 (g sediment)-1. The sediment-sample collected from 275 cmbsf (centimeters beneath the seafloor) of the 530-mbsl-core yielded many such obligately aerobic isolates belonging to Cereibacter, Guyparkeria, Halomonas, Methylophaga, Pseudomonas and Sulfitobacter which died upon anaerobic incubation, despite being provided with all possible electron acceptors and fermentative substrates. High percentages of metatranscriptomic reads from the 275 cmbsf sediment-sample, and metagenomic reads from all 25 sediment-samples, matched the isolates’ genomic sequences including those for aerobic metabolisms, genetic/environmental information processing and cell division, thereby illustrating the bacteria's in-situ activity, and ubiquity across the sediment-horizons, respectively. The findings hold critical implications for organic carbon sequestration/remineralization, and inorganic compounds oxidation, within the sediment realm of global marine OMZs.
Treatment issues in recurrentClostridioides difficileinfections and the possible role of germinants
Authors: Budi Noah , Safdar Nasia , Rose Warren E 
Published: September 2020

ABSTRACTClostridioides difficile is the number one cause of hospital-acquired infections in the United States and one of the CDC's urgent-level pathogen threats. The inflammation caused by pathogenic C. difficile results in diarrhea and pseudomembranous colitis. Patients who undergo clinically successful treatment for this disease commonly experience recurrent infections. Current treatment options can eradicate the vegetative cell form of the bacteria but do not impact the spore form, which is impervious to antibiotics and resists conventional environmental cleaning procedures. Antibiotics used in treating C. difficile infections (CDI) often do not eradicate the pathogen and can prevent regeneration of the microbiome, leaving them vulnerable to recurrent CDI and future infections upon subsequent non-CDI-directed antibiotic therapy. Addressing the management of C. difficile spores in the gastrointestinal (GI) tract is important to make further progress in CDI treatment. Currently, no treatment options focus on reducing GI spores throughout CDI antibiotic therapy. This review focuses on colonization of the GI tract, current treatment options and potential treatment directions emphasizing germinant with antibiotic combinations to prevent recurrent disease.
Antidumping Duty Investigations and Decisions in the Global Forest Products Industry
Authors: Zhang Xufang , Sun Changyou , Gordon Jason , Li Cheng , Munn Ian A 
Published: September 2020

Abstract

Special tariffs have been increasingly adopted to protect domestic industries from potential injury caused by unfair international trade. In this study, a multinomial logit model is employed to examine the patterns and determinants of antidumping duty investigations in the global forest products industry. From 1995 to 2015, a total of 372 relevant cases are identified. The number of firms and the inclusion of unions as a petitioner generally improve the probability of an affirmative decision. The characteristics of petition countries have shown more impacts on the decisions than those of target countries. Developing countries have initiated a larger number of antidumping duty investigations in the global forest products industry, and furthermore, they are more inclined to adopt antidumping duties than developed countries. Countries with abundant forest resources and more forest products trade are more likely to make an affirmative decision. These findings will provide helpful information to industrial firms and government agencies in dealing with the challenges of global competition.

Study Implications
The findings reveal that getting more stakeholders involved in an antidumping duty investigation will increase the probability of trade intervention. The economic status in petition countries is the primary determinant of the decisions from antidumping duty investigations in the global forest products industry. Developing countries not only have initiated a large number of antidumping duty investigations in the forest products industry but also have been more inclined to adopt antidumping duties. Antidumping duty investigations offer an opportunity to countries with abundant forest resources and extensive forest products trade in case of unfair international competition.
Good practices in PSHA: declustering, b-value estimation, foreshocks and aftershocks inclusion; a case study in Italy
Authors: Taroni Matteo , Akinci Aybige 
Published: September 2020

SUMMARYThe classical procedure of the probabilistic seismic hazard analysis (PSHA) requires a Poissonian distribution of earthquakes. Seismic catalogues follow a Poisson distribution just after the application of a declustering algorithm that leaves only one earthquake for each seismic sequence (usually the stronger, i.e. the main shock). Removing earthquakes from the seismic catalogues leads to underestimation of the annual rates of the events and consequently associate with low seismic hazard as indicated by several studies. In this study, we aim investigating the performance of two declustering methods on the Italian instrumental catalogue and the impact of declustering on estimation of the b-value and on the seismic hazard analysis. To this end, first the spatial variation in the seismicity rate was estimated from the declustered catalogues using the adaptive smoothed seismicity approach, considering small earthquakes (Mw ≥ 3.0). We then corrected the seismicity rates using new approach that allows for counting all events in the complete seismic catalogue by simply changing the magnitude frequency distribution. The impact of declustering on seismic hazard analysis is illustrated using PSHA maps in terms of peak ground acceleration and spectral acceleration in 2 s, with 10 per cent and 2 per cent probability of exceedance in 50 yr, for Italy. We observed that the hazard calculated from the declustered catalogues was always lower than the hazard computed using the complete catalogue. These results are in agreement with previous results obtained in different parts of the world.
Does Issue Importance Predict Learning About Candidates?
Authors: Robison Joshua 
Published: September 2020

Abstract
A long-standing argument is that policy voting is more likely on issues the voter considers subjectively important. However, existing evidence is highly mixed. We leverage panel data from the 2008–2009 ANES Panel to investigate the relationship between subjective issue importance and a key mechanism thought to link it and policy voting: candidate knowledge. Using both lagged dependent variable and fixed-effect models, we find little evidence that subjective issue importance predicts candidate knowledge or learning. Our results suggest that one reason for lack of consensus about whether subjective issue importance moderates policy voting is the lack of a clear connection between it and this important mediator. Our results point to the need for stronger measures of subjective issue importance.
Pharmacokinetics of Mephedrone and Its Metabolites in Whole Blood and Plasma after Controlled Intranasal Administration to Healthy Human Volunteers
Authors: Czerwinska Joanna , Parkin Mark C , George Claire , Kicman Andrew T , Dargan Paul I , Abbate Vincenzo 
Published: September 2020

Abstract
Mephedrone is a popular synthetic cathinone, known for its psychostimulant effects. At present, there is no data available on the pharmacokinetics of mephedrone and its metabolites in concurrently collected whole blood and plasma samples after a controlled intranasal administration to healthy volunteers. In this study, six healthy male volunteers nasally insufflated 100 mg of pure mephedrone hydrochloride (Day 1). Whole blood and plasma samples were collected at different time points after the administration and were analyzed for the presence of mephedrone and its metabolites, dihydro-mephedrone (DHM), nor-mephedrone (NOR), hydroxytolyl-mephedrone (HYDROXY), 4-carboxy-mephedrone (4-CARBOXY) and dihydro-nor-mephedrone (DHNM), by validated liquid chromatography–tandem mass spectrometry methods. All analytes were detected in whole blood and plasma for 6 h post administration, with mephedrone and NOR also being detectable on Day 2 in some participants. 4-CARBOXY, followed by NOR, was the most abundant metabolite in both matrices. Compared to other psychostimulants, mephedrone showed rapid absorption (mean Tmax of 52.5 ± 20.7 min in plasma and 55.0 ± 18.2 min in whole blood) and elimination (mean t1/2 of 1.98 ± 0.30 h in plasma and 2.12 ± 0.33 h in whole blood). In addition, statistical analysis showed that median whole blood to plasma distribution ratios, reported here for the first time, were statistically different from 1 (unity) for mephedrone (median: 1.11), DHM (median: 1.30) and NOR (median: 0.765). It is hoped that the study will aid forensic and clinical toxicologists in detection, identification and interpretation of cases associated with mephedrone use.
Knockdown ofcalreticulin, laccase, andSnf7Genes Through RNAi Is Not Effective to Control the Asian Citrus Psyllid (Hemiptera: Livideae)
Authors: Angelotti-Mendonça Jéssika , Bassan Meire M , Marques João Paulo R , Yamamoto Pedro T , Figueira Antonio , Piedade Sônia Maria De S , Mourão Filho Francisco A A 
Published: September 2020

AbstractThe Asian citrus psyllid, Diaphorina citri Kuwayama, transmits the bacteria Candidatus Liberibacter associated with huanglongbing (HLB), a devastating disease of the citrus industry. The use of genetically modified plants is an alternative to control this vector. Conversely, technology based on RNA interference (RNAi) for silencing specific genes of a target insect could be attempted. This work evaluated the knockdown effect of the target genes calreticulin (DcCRT), laccase (DcLAC), and Snf7 (DcSnf7) by RNAi through feeding D. citri in Murraya paniculata leaves after the uptake of an aqueous solution with dsRNA homologous to each vector target gene. Confocal microscopy revealed the uptake of the fluorescent-labeled dsRNA by detached leaves and the symplastic movement, allowing the ingestion by the feeding insect. A reduction in the survival rate was observed only 144 h after the beginning of feeding with dsRNA targeting DcSnf7; however, no reduction in transcript accumulation. The knockdown of the DcCRT and DcLAC genes was detected only 12 and 96 h after insect feeding, respectively. Additionally, a reduction in amino acid excretion from insects fed with dsRNA targets to DcCRT and DcLAC was observed 120 h after the beginning of feeding. However, the effects of the dsRNAs tested here appear to be minimal, both at the transcriptional and phenotype levels. For most concentrations and time points, no effects were observed. Therefore, the knockdown of genes DcCRT, DcLAC, and DcSnf7 do not appear to have the potential to control of D. citri through RNAi-mediated gene silencing.
Imputing single-cell RNA-seq data by considering cell heterogeneity and prior expression of dropouts
Authors: Zhang Lihua , Zhang Shihua 
Published: September 2020

AbstractSingle-cell RNA sequencing (scRNA-seq) provides a powerful tool to determine expression patterns of thousands of individual cells. However, the analysis of scRNA-seq data remains a computational challenge due to the high technical noise such as the presence of dropout events that lead to a large proportion of zeros for expressed genes. Taking into account the cell heterogeneity and the relationship between dropout rate and expected expression level, we present a cell sub-population based bounded low-rank (PBLR) method to impute the dropouts of scRNA-seq data. Through application to both simulated and real scRNA-seq datasets, PBLR is shown to be effective in recovering dropout events, and it can dramatically improve the low-dimensional representation and the recovery of gene‒gene relationships masked by dropout events compared to several state-of-the-art methods. Moreover, PBLR also detects accurate and robust cell sub-populations automatically, shedding light on its flexibility and generality for scRNA-seq data analysis.
Proximity to Oil Refineries and Risk of Cancer: A Population-Based Analysis
Authors: Williams Stephen B , Shan Yong , Jazzar Usama , Kerr Preston S , Okereke Ikenna , Klimberg V Suzanne , Tyler Douglas S , Putluri Nagireddy , Lopez David S , Prochaska John D , Elferink Cornelis , Baillargeon Jacques G , Kuo Yong-Fang , Mehta Hemalkumar B 
Published: September 2020

Abstract

Background
The association between proximity to oil refineries and cancer rate is largely unknown. We sought to compare the rate of cancer (bladder, breast, colon, lung, lymphoma, and prostate) according to proximity to an oil refinery in Texas.

Methods
A total of 6 302 265 persons aged 20 years or older resided within 30 miles of an oil refinery from 2010 to 2014. We used multilevel zero-inflated Poisson regression models to examine the association between proximity to an oil refinery and cancer rate.

Results
We observed that proximity to an oil refinery was associated with a statistically significantly increased risk of incident cancer diagnosis across all cancer types. For example, persons residing within 0-10 (risk ratio [RR] = 1.13, 95% confidence interval [CI] = 1.07 to 1.19) and 11-20 (RR = 1.05, 95% CI = 1.00 to 1.11) miles were statistically significantly more likely to be diagnosed with lymphoma than individuals who lived within 21-30 miles of an oil refinery. We also observed differences in stage of cancer at diagnosis according to proximity to an oil refinery. Moreover, persons residing within 0-10 miles were more likely to be diagnosed with distant metastasis and/or systemic disease than people residing 21-30 miles from an oil refinery. The greatest risk of distant disease was observed in patients diagnosed with bladder cancer living within 0-10 vs 21-30 miles (RR = 1.30, 95% CI = 1.02 to 1.65), respectively.

Conclusions
Proximity to an oil refinery was associated with an increased risk of multiple cancer types. We also observed statistically significantly increased risk of regional and distant/metastatic disease according to proximity to an oil refinery.
Women, Peace, and Security: Mapping the (Re)Production of a Policy Ecosystem
Authors: Kirby Paul , Shepherd Laura J 
Published: September 2020

Abstract
The Women, Peace, and Security (WPS) agenda is a global peace and security architecture conventionally understood as emerging from a suite of UN Security Council resolutions and accompanying member state action plans over the last twenty years. The agenda serves as a major international gender equality initiative in its own right and as a prominent example of the broadening of security practices in global politics. In this paper, we present the first truly systematic analysis of the agenda, drawing on a novel dataset of 213 WPS policy documents from across the UN system, national government initiatives, and regional and international organizations published between 2000 and 2018. We argue that the degree of variation in the WPS agenda is frequently underestimated in conventional models of norm diffusion and policy transfer, and instead propose an account of the agenda as a dynamic ecosystem shaped by reproduction and contestation. Our empirical mapping runs counter to established narratives about the development of the agenda, producing insights into the pace and location of the growth of WPS; the hierarchy of its key “pillars”; the emergence of new issues; the development of rival versions of the agenda; and the role of domestic institutions in shaping WPS policy. We find support for the claim that the WPS agenda is pluralizing in significant ways and provide illustrations of points of fracture within the agenda at large. Our argument has significant implications for the WPS research agenda and for scholarship on security norms and policy more broadly.