bluuebunny/crossref_metadata_2025_split huggingface dataset:
Assessment of ponds surrounding Imphal Valley on the basis of various biological and physicochemical parameters
Authors: Singh Soubam Indrakumar , Sharma Priyanka , Chaudhary Savita , Sharma Mon Gonchandra , Yegemova Saltanat , Kumar Rajeev 
Published: November 2019

AbstractThe water quality is one of the major criteria for the environmental evaluation based on the parametric changes in the physicochemical and biological properties of water. The distribution patterns of physicochemical and biological characteristics of the various rivers in the Imphal Valley have been studied in this work. The objective of the work is to determine the surface water quality of Imphal Valley to assess its suitability for domestic purposes. The physicochemical parameters of water such as water temperature, pH, hardness, alkalinity, EC, TDS, chloride, calcium, magnesium, sodium, potassium and nitrate were analysed for the experimentation. The biological characteristics were estimated from the Total coliform and Escherichia coli content of water. The physicochemical parameters were within the acceptable limits on the other hand Total coliform, and E. coli values were exceeding from the acceptable and permissible limits as prescribed by Bureau of Indian Standards (BIS). The study suggests untreated domestic disposal from market and household to the water body causes heavy pollution in rivers of Imphal Valley.
Natural variation in the contribution of microbial density to inducible immune dynamics
Authors: Jent Derrick , Perry Abby , Critchlow Justin , Tate Ann T. 
Published: November 2019

AbstractImmune responses evolve to balance the benefits of microbial killing against the costs of autoimmunity and energetic resource use. Models that explore the evolution of optimal immune responses generally include a term for constitutive immunity, or the level of immunological investment prior to microbial exposure, and for inducible immunity, or investment in immune function after microbial challenge. However, studies rarely consider the functional form of inducible immune responses with respect to microbial density, despite the theoretical dependence of immune system evolution on microbe‐ versus immune‐mediated damage to the host. In this study, we analyse antimicrobial peptide (AMP) gene expression from seven wild‐caught flour beetle populations (Tribolium spp.) during acute infection with the virulent bacteria Bacillus thuringiensis (Bt) and Photorhabdus luminescens (P.lum) to demonstrate that inducible immune responses mediated by the humoral IMD pathway exhibit natural variation in both microbe density‐dependent and independent temporal dynamics. Beetle populations that exhibited greater AMP expression sensitivity to Bt density were also more likely to die from infection, while populations that exhibited higher microbe density‐independent AMP expression were more likely to survive P. luminescens infection. Reduction in pathway signalling efficiency through RNAi‐mediated knockdown of the imd gene reduced the magnitude of both microbe‐independent and dependent responses and reduced host resistance to Bt growth, but had no net effect on host survival. This study provides a framework for understanding natural variation in the flexibility of investment in inducible immune responses and should inform theory on the contribution of nonequilibrium host‐microbe dynamics to immune system evolution.
The TuMYB46L‐TuACO3 module regulates ethylene biosynthesis in einkorn wheat defense to powdery mildew
Authors: Zheng Hongyuan , Dong Lingli , Han Xinyun , Jin Huaibing , Yin Cuicui , Han Yali , Li Bei , Qin Huanju , Zhang Jinsong , Shen Qianhua , Zhang Kunpu , Wang Daowen 
Published: November 2019

Summary

Powdery mildew disease, elicited by the obligate fungal pathogen Blumeria graminis f.sp. tritici (Bgt), causes widespread yield losses in global wheat crop. However, the molecular mechanisms governing wheat defense to Bgt are still not well understood.
Here we found that TuACO3, encoding the 1‐aminocyclopropane‐1‐carboxylic acid (ACC) oxidase functioning in ethylene (ET) biosynthesis, was induced by Bgt infection of the einkorn wheat Triticum urartu, which was accompanied by increased ET content. Silencing TuACO3 decreased ET production and compromised wheat defense to Bgt, whereas both processes were enhanced in the transgenic wheat overexpressing TuACO3.
TuMYB46L, phylogenetically related to Arabidopsis MYB transcription factor AtMYB46, was found to bind to the TuACO3 promoter region in yeast‐one‐hybrid and EMSA experiments. TuMYB46L expression decreased rapidly following Bgt infection. Silencing TuMYB46L promoted ET content and Bgt defense, but the reverse was observed when TuMYB46L was overexpressed.
Hence, decreased expression of TuMYB46L permits elevated function of TuACO3 in ET biosynthesis in Bgt‐infected wheat. The TuMYB46L‐TuACO3 module regulates ET biosynthesis to promote einkorn wheat defense against Bgt. Furthermore, we found four chitinase genes acting downstream of the TuMYB46L‐TuACO3 module. Collectively, our data shed a new light on the molecular mechanisms underlying wheat defense to Bgt.
Left on the shelf: Explaining the failure of public inquiry recommendations
Authors: Stark Alastair 
Published: November 2019

AbstractPublic inquiries remain the pre‐eminent mechanism for lesson‐learning after high‐profile failures. However, a regular complaint is that their recommendations get ‘shelved’. In political science, the most common explanation for this lack of implementation tells us that elites mobilize bias in order to undermine inquiry lesson‐learning. This article tests this thesis via an international comparison of inquiries in Australia, Canada, New Zealand and the UK. A series of alternative explanations for shelving emerge, which tell us that inquiry recommendations do not get implemented when: they do not respect the realities of policy transfer; they are triaged into policy refinement mechanisms; and they arrive at the ‘street level’ without consideration of local delivery capacities. These explanations tell us that the mobilization of bias thesis needs to be reworked in relation to public inquiries so that it better recognizes the complex reality of public policy in the modern state.
Froude supercritical flow processes and sedimentary structures: New insights from experiments with a wide range of grain sizes
Authors: Ono Kenya , Plink‐Björklund Piret , Eggenhuisen Joris T. , Cartigny Matthieu J. B. 
Published: November 2019

AbstractRecognition of Froude supercritical flow deposits in environments that range from rivers to the ocean floor has triggered a surge of interest in their flow processes, bedforms and sedimentary structures. Interpreting these supercritical flow deposits is especially important because they often represent the most powerful flows in the geological record. Insights from experiments are key to reconstruct palaeo‐flow processes from the sedimentary record. So far, all experimentally produced supercritical flow deposits are of a narrow grain‐size range (fine to medium sand), while deposits in the rock record often consist of a much wider grain‐size distribution. This paper presents results of supercritical‐flow experiments with a grain‐size distribution from clay to gravel. These experiments show that cyclic step instabilities can produce more complex and a larger variety of sedimentary structures than the previously suggested backsets and ‘scour and fill’ structures. The sedimentary structures are composed of irregular lenses, mounds and wedges with backsets and foresets, as well as undulating planar to low‐angle upstream and downstream dipping laminae. The experiments also demonstrate that the Froude number is not the only control on the sedimentary structures formed by supercritical‐flow processes. Additional controls include the size and migration rate of the hydraulic jump and the substrate cohesion. This study further demonstrates that Froude supercritical flow promotes suspension transport of all grain sizes, including gravels. Surprisingly, it was observed that all grain sizes were rapidly deposited just downstream of hydraulic jumps, including silt and clay. These results expand the range of dynamic mud deposition into supercritical‐flow conditions, where local transient shear stress reduction rather than overall flow waning conditions allow for deposition of fines. Comparison of the experimental deposits with outcrop datasets composed of conglomerates to mudstones, shows significant similarities and highlights the role of hydraulic jumps, rather than overall flow condition changes, in producing lithologically and geometrically complex stratigraphy.
Schizophyllum commune sinusitis after allogeneic bone marrow transplantation for myelodysplastic syndrome: A case report and literature review
Authors: Narazaki Taisuke , Nakashima Yasuhiro , Tsukamoto Yasuhiro , Nishida Ruriko , Tsuda Mariko , Muta Hiroki , Kimura Daisaku , Masuda Toru , Takamatsu Akiko , Kohashi Kenichi , Murakami Daisuke , Shiratsuchi Motoaki , Ogawa Yoshihiro 
Published: November 2019

AbstractSinusitis is a serious infectious complication of allogeneic hematopoietic stem cell transplantation. Schizophyllum commune (S commune) is a common basidiomycete fungus that is rarely involved in human disease. We report herein a case of S commune sinusitis after allogeneic bone marrow transplantation. A 66‐year‐old man with myelodysplastic syndrome underwent allogeneic bone marrow transplantation and developed maxillary and ethmoid sinusitis. The sinusitis did not improve with liposomal amphotericin B after neutrophil engraftment, so we considered that surgical intervention was needed for the recovery of sinusitis. Endoscopic sinus surgery was performed. In the debridement tissue of paranasal mucosa, filamentous fungal elements were observed. Moreover, genetic analysis of the tissue revealed the presence of S commune. Schizophyllum commune should be recognized as a fungal pathogen that causes sinusitis after allogeneic hematopoietic stem cell transplantation. This case suggests the effectiveness of prompt surgical intervention with liposomal amphotericin B treatment for S commune sinusitis and the usefulness of genetic diagnosis for cases under antifungal treatment. (160 words)
Can a family medicine rotation improve medical students’ knowledge, skills and attitude towards primary care in Vietnam? A pre‐test–post‐test comparison and qualitative survey
Authors: Thai Thuy T. N. , Pham Tam T. , Nguyen Kien T. , Nguyen Phuong M. , Derese Anselme 
Published: November 2019

AbstractObjectivesWell‐designed studies on the impact of a family medicine rotation on medical students are rare, and very few studies include a qualitative component. This study aimed to determine the improvement of medical students’ knowledge, communication skills and attitude towards primary care and explore their perceptions after rotations, in comparison with a control group.MethodsWe used a mixed‐methods design, comprising a pre‐test–post‐test comparison between a sample of trained students who took family medicine rotations and a control group and a qualitative survey. The measurement of improvement included (i) multiple choice question testing, (ii) objective structured checklist examinations, (iii) self‐reporting and (iv) interviews and focus group discussions. Data were collected from August 2017 to June 2018.ResultsThere were 696 students in the trained group and 617 controls. The two groups’ baseline scores in knowledge, communication skills and attitude were not significantly different. Knowledge covering five domains of family medicine (Pearson's r from 0.6 to 0.9) improved significantly, as did attitudes towards primary care in the trained group. There were no differences in communication and counselling skills between the two groups for four situations, but for two–health check‐ups and mental health care–skills were significantly improved (Pearson's r from 0.28 to 0.43). The qualitative survey showed highly positive feedback from trained students.ConclusionsThe family medicine rotation significantly improved students’ knowledge and attitude towards primary care and some communication skills. Further studies should investigate students’ interest in and career choice for this discipline.
A bioengineered living cell construct activates metallothionein/zinc/MMP8 and inhibits TGFβ to stimulate remodeling of fibrotic venous leg ulcers
Authors: Stone Rivka C. , Stojadinovic Olivera , Sawaya Andrew P. , Glinos George D. , Lindley Linsey E. , Pastar Irena , Badiavas Evangelos , Tomic‐Canic Marjana 
Published: November 2019

ABSTRACTVenous leg ulcers (VLU) represent a major clinical unmet need, impairing quality of life for millions worldwide. The bioengineered bilayered living cell construct (BLCC) is the only FDA‐approved therapy demonstrating efficacy in healing chronic VLU, yet its in vivo mechanisms of action are not well understood. Previously, we reported a BLCC‐mediated acute wounding response at the ulcer edge; in this study we elucidated the BLCC‐specific effects on the epidermis‐free ulcer bed. We conducted a randomized controlled clinical trial (ClinicalTrials.gov NCT01327937) enrolling 30 subjects with nonhealing VLUs, and performed genotyping, genomic profiling, and functional analysis on wound bed biopsies obtained at baseline and 1 week after treatment with BLCC plus compression or compression therapy (control). The VLU bed transcriptome featured processes of chronic inflammation and was strikingly enriched for fibrotic/fibrogenic pathways and gene networks. BLCC application decreased expression of profibrotic TGFß1 gene targets and increased levels of TGFß inhibitor decorin. Surprisingly, BLCC upregulated metallothioneins and fibroblast‐derived MMP8 collagenase, and promoted endogenous release of MMP‐activating zinc to stimulate antifibrotic remodeling, a novel mechanism of cutaneous wound healing. By activating a remodeling program in the quiescent VLU bed, BLCC application shifts nonhealing to healing phenotype. As VLU bed fibrosis correlates with poor clinical healing, findings from this study identify the chronic VLU as a fibrotic skin disease and are first to support the development and application of antifibrotic therapies as a successful treatment approach.
Short‐wave infrared light imaging measures tissue moisture and distinguishes superficial from deep burns
Authors: Mironov Sergey , Hwang Charles D. , Nemzek Jean , Li John , Ranganathan Kavitha , Butts Jonathan T. , Cholok David J. , Dolgachev Vladislav A. , Wang Stewart C. , Hemmila Mark , Cederna Paul S. , Morris Michael D. , Berenfeld Omer , Levi Benjamin 
Published: November 2019

AbstractExisting clinical approaches and tools to measure burn tissue destruction are limited resulting in misdiagnosis of injury depth in over 40% of cases. Thus, our objective in this study was to characterize the ability of short‐wave infrared (SWIR) imaging to detect moisture levels as a surrogate for tissue viability with resolution to differentiate between burns of various depths. To accomplish our aim, we constructed an imaging system consisting of a broad‐band Tungsten light source; 1,200‐, 1,650‐, 1,940‐, and 2,250‐nm wavelength filters; and a specialized SWIR camera. We initially used agar slabs to provide a baseline spectrum for SWIR light imaging and demonstrated the differential absorbance at the multiple wavelengths, with 1,940 nm being the highest absorbed wavelength. These spectral bands were then demonstrated to detect levels of moisture in inorganic and in vivo mice models. The multiwavelength SWIR imaging approach was used to diagnose depth of burns using an in vivo porcine burn model. Healthy and injured skin regions were imaged 72 hours after short (20 seconds) and long (60 seconds) burn application, and biopsies were extracted from those regions for histologic analysis. Burn depth analysis based on collagen coagulation histology confirmed the formation of superficial and deep burns. SWIR multispectral reflectance imaging showed enhanced intensity levels in long burned regions, which correlated with histology and distinguished between superficial and deep burns. This SWIR imaging method represents a novel, real‐time method to objectively distinguishing superficial from deep burns.
Exercise and mitochondrial health
Authors: Memme Jonathan M. , Erlich Avigail T. , Phukan Geetika , Hood David A. 
Published: November 2019

AbstractMitochondrial health is an important mediator of cellular function across a range of tissues, and as a result contributes to whole‐body vitality in health and disease. Our understanding of the regulation and function of these organelles is of great interest to scientists and clinicians across many disciplines within our healthcare system. Skeletal muscle is a useful model tissue for the study of mitochondrial adaptations because of its mass and contribution to whole body metabolism. The remarkable plasticity of mitochondria allows them to adjust their volume, structure and capacity under conditions such as exercise, which is useful or improving metabolic health in individuals with various diseases and/or advancing age. Mitochondria exist within muscle as a functional reticulum which is maintained by dynamic processes of biogenesis and fusion, and is balanced by opposing processes of fission and mitophagy. The sophisticated coordination of these events is incompletely understood, but is imperative for organelle function and essential for the maintenance of an interconnected organelle network that is finely tuned to the metabolic needs of the cell. Further elucidation of the mechanisms of mitochondrial turnover in muscle could offer potential therapeutic targets for the advancement of health and longevity among our ageing populations. As well, investigating exercise modalities that are both convenient and capable of inducing robust mitochondrial adaptations are useful in fostering more widespread global adherence. To this point, exercise remains the most potent behavioural therapeutic approach for the improvement of mitochondrial health, not only in muscle, but potentially also in other tissues.
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An Inverse Design Method of Buckling-Guided Assembly for Ribbon-Type 3D Structures
Authors: Xu Zheng , Fan Zhichao , Zi Yanyang , Zhang Yihui , Huang Yonggang 
Published: November 2019

Abstract
Mechanically guided three-dimensional (3D) assembly based on the controlled buckling of pre-designed 2D thin-film precursors provides deterministic routes to complex 3D mesostructures in diverse functional materials, with access to a broad range of material types and length scales. Existing mechanics studies on this topic mainly focus on the forward problem that aims at predicting the configurations of assembled 3D structures, especially ribbon-shaped structures, given the configuration of initial 2D precursor and loading magnitude. The inverse design problem that maps the target 3D structure onto an unknown 2D precursor in the context of a prescribed loading method is essential for practical applications, but remains a challenge. This paper proposes a systematic optimization method to solve the inverse design of ribbon-type 3D geometries assembled through the buckling-guided approach. In addition to the torsional angle of the cross section, this method introduces the non-uniform width distribution of the initial ribbon structure and the loading mode as additional design variables, which can significantly enhance the optimization accuracy for reproducing the desired 3D centroid line of the target ribbon. Extension of this method allows the inverse design of entire 3D ribbon configurations with specific geometries, taking into account both the centroid line and the torsion for the cross section. Computational and experimental studies over a variety of elaborate examples, encompassing both the single-ribbon and ribbon-framework structures, demonstrate the effectiveness and applicability of the developed method.
Field-trip guide to the Catalina-Rincon metamorphic core complex, Tucson, Arizona
Authors: Davis George H. , Spencer Jon E. , Gehrels George E. 
Published: November 2019

ABSTRACT
The Santa Catalina and Rincon Mountains north and east of Tucson, Arizona, form one of the largest core complexes on Earth. Both ranges consist primarily of Eocene leucogranites that intrude Proterozoic and late Cretaceous granitoids, and two Oligocene plutons. Mylonitic fabrics are well developed on the southern flank of the Santa Catalina Mountains and the southwestern flank of the Rincon Mountains. The corrugated form of the two ranges reflects the grooved form of the ca. 15–30 Ma Catalina–San Pedro detachment fault exposed primarily at the foot of the ranges. Normal displacement on two younger high-angle normal faults is responsible for much of the substantial relief of the ranges. This field guide is focused on fault rocks and mylonitic fabrics in the footwalls of the detachment fault and the high-angle Pirate normal fault, and includes description and analysis of shear-zone kinematics and processes, U-Pb geochronology of leucogranites, and core-complex geomorphology.
Accessibility and inclusion in the field: A field guide for central Arizona and Petrified Forest National Park
Authors: Atchison Christopher L. , Parker William G. , Riggs Nancy R. , Semken Steven , Whitmeyer Steven J. 
Published: November 2019

ABSTRACT
This field trip focuses on accessible and inclusive design in field-based teaching and learning through a broad investigation of the geology of Arizona, followed by more detailed exercises that focus on the Upper Triassic stratigraphic sequences in Petrified Forest National Park (PEFO). The first day of the field trip will traverse the three physiographic provinces of Arizona, from fault-bounded, basement-cored uplifts and valleys of the Basin and Range in the greater Phoenix area, through the Transition Zone to the Mogollon Rim, and ending in Upper Triassic sedimentary rocks of the Colorado Plateau at Holbrook. The second day of the field trip will encompass more detailed, collaborative exercises in PEFO that utilize the expertise of both student and faculty participants in mixed-ability groups. The main priority of this accessible field experience is the development of an inclusive community of learning driven by paired student-faculty interactions, facilitated as needed by technology integration to mitigate barriers and foster engagement, communication, and collaboration across a spectrum of ability and content knowledge.
Please note that the collection of archaeological artifacts, fossils, rocks, or other natural history objects without an active research and collection permit is illegal at Petrified Forest National Park. Please refrain from collecting samples or specimens of any kind from anywhere in the park.