bluuebunny/crossref_metadata_2025_split huggingface dataset:
Synthesis of Nitrogenase by Paenibacillus Sabinae T27 in Presence of High Levels of Ammonia During Anaerobic Fermentation
Authors: Li Qin , He Xiao-Juan , Liu Peng-Xi , Zhang Hao-Wei , Wang Ming-Yang , Chen San-Feng 
Published: November 2020

Abstract
BackgroundBiological nitrogen fixation catalyzed by nitrogenase is a high energy-intensive process, and thus nitrogenase synthesis and activity are inhibited by ammonium (NH4+). Microorganism fix nitrogen at high ammonium (30-300 mM) concentration has not been reported before.ResultsPaenibacillus sabinae T27, a Gram-positive, spore-forming diazotroph (N2-fixing microorganism, showed nitrogenase activities not only in low (0-4 mM) concentration of NH4+, but also in high (30-300 mM) concentration of NH4+, no matter whether the cells of this bacterium were grown in flask or in fermentor on scale cultivation. qRT-PCR and western blotting analysis supported that Fe protein and MoFe protein were synthesized under both low (0-4 mM) and high (30-300 mM) concentration of NH4+. Liquid chromatography-mass spectrometry（LC-MS）analysis revealed that MoFe protein purified form cultures grown in nitrogen-limited condition or nitrogen-excess condition was encoded by nifDK and Fe protein was encoded by both nifH and nifH2. The cross-reaction suggested the purified Fe and MoFe components from P. sabinae T27 grown in both nitrogen-limited and -excess conditions were active.ConclusionsOur results indicate that N2 fixation occurs in presence of high (30-300 mM) concentration of NH4+ in P. sabinae T27. Nitrogen fixation under both low and high concentration of NH4+ was catalyzed by the same nitrogenases and the Fe protein was encoded by both nifH and nifH2. Our study will provide a clue for studying the mechanisms on nitrogen fixation in presence of the high concentration of NH4+.
Combining CBP Pharmacophore Construction and Molecular Docking to Search for Potential Competitive Inhibitors of Chitin Deacetylase
Authors: Tang Zizhong , Fu Xiaoli , Huang Lu , Wang Haoxiang , Tang Biao , Xiao Yirong , Wan Yujun , Chen Hui , Yao Huipeng , Shan Zhi , Wang Gang 
Published: November 2020

Abstract
Chitin deacetylase (CDA) is a key enzyme for plant pathogens to evade host defense recognition. However, in the study of CDA inhibitors, only chitin deacetylase from colletotrichum lindemuthianum (ClCDA) was found to participate in the reverse hydrolysis reaction in sodium acetate to acetylate free amino sugar residues into N-acetylated forms. Based on this, we selected 10,632 small molecules from the DrugBank database for computer virtual screening to find new potential CDA inhibitors. First, we use the CBP model with ROC = 0.800 to coarsely screen small molecules. Then we use the LibDock and CDOCKER programs in Discovery Studio 2016 (DS 2016) to dock the best-matched small molecules to identify interactions with key residues on the active site of ClCDA. Finally, we found two potential compounds with good adaptability, high docking score and important interactions with protein active sites. And we confirm that their structures are stable and have multiple non-bonding interactions with important amino acid sites such as ASP50, TYR145, HIS206 and ZN1255 by MD simulations. Therefore, we conclude that the selected compounds are likely to be new inhibitors of CDA. In this research could provide a valuable resource and guidance for CDA-related inhibitors development.
Pregnancy-Related Acute Kidney Injury at High Altitude: A Retrospective Observational Study in a Single Center
Authors: Wang Weiming , Li Xin , Wu Xiaojing , Zhang Muyin , Xu Lili , Li Guohui , Wen Yumei 
Published: November 2020

Abstract
BackgroundPregnancy-related acute kidney injury (Pr-AKI) is associated with maternal and fetal morbidity and mortality. There are few studies focusing on Pr-AKI at high altitude in the literature. Objectivesto investigate the incidence, etiology, clinical features and maternal-fetal outcomes of Pr-AKI in women living at high altitude.Methods6,512 pregnant women attending the Department of Obstetrics＆Gynecology at local hospital from January 2015 to December 2018 were screened for Pr-AKI. Patients with serum creatinine above normal range(>70umol/L) then underwent assessment to confirm the diagnosis of Pr-AKI. AKI was diagnosed and staged based on Kidney Disease Improving Global Outcomes(KDIGO) guideline. Individuals meeting the Pr-AKI criteria were recruited. Their clinical data were recorded and retrospectively analyzed. ResultsPr-AKI was identified in 136/6512(2.09%) patients. Hypertensive disorders of pregnancy(HDP) was the leading cause of Pr-AKI(35.3%). 4(2.9%) women died and the majority(86.1%) had recovered renal function before discharge. Fetal outcomes were confirmed in 109 deliveries with gestational age≥20 weeks. Pre-term delivery occurred in 30(27.3%) cases and perinatal deaths in 17(15.5%). The rate of low birth weight infant(LBWI) and intrauterine growth restriction(IUGR) was 22.0% and 10.9% respectively. 16(14.5%) infants were admitted to NICU after birth. Patients with HDP had a higher cesarean rate(56.3%). More IUGR(25.0%) and LBWI(37.8%) were observed in their infants with a higher risk of admission to NICU(22.0%). High altitude might have an adverse impact on HDP-related Pr-AKI patients with earlier terminated pregnancy and more stillbirth/neonatal death. Logistic regression models indicated that uncontrolled blood pressure, high altitude and advanced AKI were associated with adverse fetal outcomes in HDP-related Pr-AKI patients.ConclusionsPr-AKI was not rare in high-altitude regions and caused severe fetal morbidities and mortalities. Uncontrolled blood pressure, high altitude and advanced AKI were all risk factors for adverse fetal outcomes in Pr-AKI patients, especially for those with hypertensive disorders of pregnancy.
The Fulminant Index: A Method of Rapidly Differentiating Fulminant Type 1 Diabetes From Diabetic Ketoacidosis
Authors: Qiu Junlin , Li Xia , Chen Wen , Ma Xiaoxi , Xie Zhiguo , Huang Gan , Luo Shuoming , Zhou Zhiguang 
Published: November 2020

Abstract
Objectives Fulminant type 1 diabetes (FT1D) usually presents with diabetes ketoacidosis(DKA) at the disease onset. Early identification of FT1D is crucial. This study was aimed at investigating whether the fulminant index (FI), including plasma glucose (PG) to glycated hemoglobin(HbA1c) ratio (PG/HbA1c), serum potassiumion (K+) to HbA1c ratio (K+/HbA1c), and serum sodiumion (Na+) multiplied by HbA1c (Na+*HbA1c), is a suitable indicator for early FT1D identification.Methods A total of 76 subjects were enrolled, including 40 FT1D patients and 36 non-FT1D patients with DKA. We utilized receiver operating characteristic (ROC) curve analysis to determine the FI cut-off values between FT1D and non-FT1D groups.Results ROC curve analyses showed that the maximum Youden’s index for PG/HbA1c bonding to a cut-off value of 4.39, with the sensitivity of 75.0% and specificity of 77.8% in identifying FT1D from DKA. And optimal K+/HbA1c cut-off value was 0.85 with the sensitivity of 77.5% and specificity of 94.4%. For Na*HbA1c, the best cut-off value was 923.65, and its sensitivity and specificity were 85% and 69.4%, respectively.Conclusions These results suggested FI could work as a suitable and convenient indicator for differentiating FT1D from initial DKA patients, among which the FI (K+/HbA1c) presented the best .
Neuroprotective Effects of Intraoperative Dexmedetomidine Infusion Combined With Goal-directed Hemodynamic Therapy for Patients Undergoing Cranial Surgery: A Double-blinded Randomized Controlled Trial
Authors: Chen Pin-Hsin , Tsuang Fon-Yih , Lee Chen-Tse , Yeh Yu-Chang , Cheng Hsiao-Liang , Lee Tzong-Shiun , Chang Ya-Wen , Cheng Ya-Jung , Wu Chun-Yu 
Published: November 2020

Abstract
BackgroundDespite dexmedetomidine may be neuroprotective in patients undergoing cranial surgery by inhibiting neuroinflammation; however, it reduces cardiac output and cerebral blood flow. We proposed that dexmedetomidine infusion combined with goal-directed hemodynamic therapy (GDHT) could improve cranial surgery neurological outcomes without hemodynamic perturbation.MethodsA randomized, double-blind trial was conducted. One hundred sixty adult patients undergoing elective cranial surgery received either dexmedetomidine (0.5 μg kg−1 h−1) or saline during surgery. The goal-directed hemodynamic therapy was used for stroke volume optimization. The proportion of patients who developed postoperative new neurological deficits was compared. The severities of new neurological deficit were assessed by using in-hospital Barthel index changes and the 30-day modified Rankin Scale (mRS). Postoperative delirium was identified using the Intensive Care Delirium Screening Checklist (ICSDC) criteria. The level of a perioperative serum neuroinflammatory mediator, high motility group box 1 protein (HMGB1), was compared.ResultsThe dexmedetomidine group exhibited a lower cardiac index than did the control group (3.0 ± 0.8 vs. 3.4 ± 1.8 L min−1 m−2; p = 0.0482) without lactate accumulation. Fewer patients in the dexmedetomidine group developed new postoperative deficits (26.3% versus 43.8%; p = 0.031) but numbers of patients remained symptomatic neurological deficit before discharge were comparable between the two groups (23.8% vs. 38.8%; p= 0.060). In addition, an attenuated Barthel index decline (−6.3 ± 20.4 vs. −13.6 ± 24.8; p = 0.043), a more favorable 30-day mRS profile (p = 0.013), and a higher incidence of postoperative delirium-free (ICDSC scored 0: 84.6% versus 64.2%; p = 0.012) were observed in the dexmedetomidine group. Furthermore, dexmedetomidine induced a significant decline in perioperative serum HMGB1 level (222.5 ± 408.3 vs. 152.2 ± 280.0 ng mL−1; p = 0.0033).ConclusionsDexmedetomidine infusion combined with GDHT mitigates neuroinflammation during cranial surgery without hemodynamic perturbation, thus achieving neuroprotective effects.Clinical Trial RegistrationProspectively registered at clinicaltrials.gov. (identifier NCT02878707, date of registration: August 25, 2016)
Fresh frozen plasma transfusion in the acute period of isolated traumatic brain injury
Authors: Baranich A. I. , Sychev A. A. , Zakharova N. E. , Savin I. A. , Oshorov A. V. , Polupan A. A. , Latyshev Ya. A. , Potapov A. A. 
Published: November 2020

Coagulopathy associated with traumatic brain injury (TBI) is recognized as one of the risk factors for poor outcome in patients with TBI, however, the safety of using fresh frozen plasma (FFP) is not fully understood.The objective of the study: to identify the indications for FFP transfusion in the acute period of TBI.Subjects and methods: a retrospective-prospective observational study included 160 patients aged 18-59 years with isolated TBI in the first 48 hours after injury. Patients were assessed by Glasgow Coma Scale (GCS); patients were divided into two study groups: Group 1 – GCS ≤ 8 points, Group 2 – GCS ≥ 9 points. All patients underwent hemostatic assessment with standard clotting tests (activated partial thromboplastin time, prothrombin ratio, fibrinogen blood level). In 42 patients, additional thromboelastometry was performed. Specific parameters of FFP transfusion and trauma outcomes were assessed.Results: according to clotting tests, hypocoagulation was detected in 50.6% of patients; according to thromboelastometry – in 22.7%. FFP was used more often in severe TBI (83%) with a decrease in prothrombin ratio (PR). However, FFP transfusion is associated with an unfavorable outcome: in the case of transfusion, a greater number of deaths and vegetative states were recorded in patients with severe TBI.Conclusion: in patients in the acute period of isolated TBI, it is preferable to minimize the use of FFP; an isolated decrease in PR should not be a trigger for FFP transfusion.
Ventilator-associated lung injury in the intensive care unit and operating room – what's new?
Authors: Kuzkov V. V. , Lapin K. S. , Fot E. V. , Kirov M. Yu. 
Published: November 2020

The prophylaxis of ventilator-associated lung injury (VALI) and postoperative pulmonary complications (PPC) is of utmost importance to reduce complications both in the perioperative period of major surgery and in the intensive care unit (ICU).Protective approach to mechanical ventilation comprises a wide range of measures reducing the damage of the lung tissue associated with the stress and strain phenomena. The implementation of the strategy of high positive end-expiratory pressure (PEEP) in combination with alveolar recruitment maneuver has numerous limitations and requires further personalized approaches.When lung injury is self-induced by a patient, it becomes an important contributor to VALI and should be timely diagnosed and prevented both before initiation of mechanical support and during the restoration of spontaneous breathing. This review highlights the key mechanisms of VALI and current understanding of protective ventilation. The concept of damaging energy as well as approaches to the personalized optimization of respiratory settings are discussed in detail. Particular attention is paid to the prognostication of the risk factors of VALI and PPC.
PERAN ADVERSITY QUOTIENT MEMODERASI PENGARUH SELF EFFICACY DAN LINGKUNGAN KERJA TERHADAP PENERAPAN TPACK PEMBELAJARAN AKUNTANSI
Authors: Astutik Adelina 
Published: November 2020

Abstrak: Penelitian ini bertujuan untuk menguji pengaruh self efficacy dan lingkungan kerja terhadap penerapan TPACK pembelajaran akuntansi dengan adversity quotient sebagai variabel moderating. Penelitian ini menggunakan pendekatan kuantitatif. Populasi penelitian ini adalah guru SMK Program Keahlian Akuntansi. Teknik pengambilan sampel manggunakan sampel jenuh dengan total responden penelitian sejumlah 77 responden. Metode pengumpulan data menggunakan kuesioner. Teknik analisis data menggunakan analisis deskriptif dan analisis regresi dengan uji selisih mutlak. Hasil statistik deskriptif menunjukkan bahwa penerapan TPACK, self efficacy dan adversity quotient berada pada kategori tinggi, sedangkan lingkungan kerja berada pada kategori yang mendukung. Hasil penelitian menunjukkan self efficacy berpengaruh positif dan signifikan terhadap penerapan TPACK pembelajaran akuntansi, lingkungan kerja berpengaruh positif dan signifikan terhadap penerapan TPACK pembelajaran akuntansi. Uji selisih nilai mutlak menunjukkan adversity quotient mampu memperkuat pengaruh self efficacy terhadap penerapan TPACK pembelajaran akuntansi, adversity quotient tidak mampu memperkuat pengaruh lingkungan kerja terhadap penerapan TPACK pembelajaran akuntansi. Abstract: This study aims to examine the effect of self-efficacy and work environment on the application of TPACK in accounting learning with adversity quotient as avariable moderating. This study uses a quantitative approach. The population of this research is the teachers of SMK Accounting Expertise Program. The sampling technique used saturated samples with a total of 77 respondents. The data collection method used a questionnaire. The data analysis technique used descriptive analysis and regression analysis with absolute difference test. The results of descriptive statistics show that the application of TPACK, self-efficacy and adversity quotient is in the high category, while the work environment is in the supportive category. The results showed that self-efficacy had a positive and significant effect on the application of TPACK in accounting learning, the work environment had a positive and significant effect on the application of TPACK in accounting learning. The absolute value difference test shows that the adversity quotient is able to strengthen the effect of self-efficacy on the application of TPACK in accounting learning, the adversity quotient is not able to strengthen the effect of the work environment on the application of TPACK in accounting learning.
Factors Affecting Engagement in Web-Based Health Care Patient Information: Narrative Review of the Literature
Authors: Oktay Liam Alperen , Abuelgasim Eyad , Abdelwahed Aida , Houbby Nour , Lampridou Smaragda , Normahani Pasha , Peters Nicholas , Jaffer Usman 
Published: November 2020

Background
Web-based content is rapidly becoming the primary source of health care information. There is a pressing need for web-based health care content to not only be accurate but also be engaging. Improved engagement of people with web-based health care content has the potential to inform as well as influence behavioral change to enable people to make better health care choices. The factors associated with better engagement with web-based health care content have previously not been considered.

Objective
The aims of this study are to identify the factors that affect engagement with web-based health care content and develop a framework to be considered when creating such content.

Methods
A comprehensive search of the PubMed and MEDLINE database was performed from January 1, 1946, to January 5, 2020. The reference lists of all included studies were also searched. The Medical Subject Headings database was used to derive the following keywords: “patient information,” “online,” “internet,” “web,” and “content.” All studies in English pertaining to the factors affecting engagement in web-based health care patient information were included. No restrictions were set on the study type. Analysis of the themes arising from the results was performed using inductive content analysis.

Results
The search yielded 814 articles, of which 56 (6.9%) met our inclusion criteria. The studies ranged from observational and noncontrolled studies to quasi-experimental studies. Overall, there was significant heterogeneity in the types of interventions and outcome assessments, which made quantitative assessment difficult. Consensus among all authors of this study resulted in six categories that formed the basis of a framework to assess the factors affecting engagement in web-based health care content: easy to understand, support, adaptability, accessibility, visuals and content, and credibility and completeness.

Conclusions
There is a paucity of high-quality data relating to the factors that improve the quality of engagement with web-based health care content. Our framework summarizes the reported studies, which may be useful to health care content creators. An evaluation of the utility of web-based content to engage users is of significant importance and may be accessible through tools such as the Net Promoter score. Web 3.0 technology and development of the field of psychographics for health care offer further potential for development. Future work may also involve improvement of the framework through a co-design process.
PENYALAHGUNAAN ZAT ADIKTIF OLEH ANAK DI BAWAH UMUR (Studi Kasus di Kecamatan Blangkejeren Kabupaten Gayo Lues)
Authors: Yahya Faisal , Fadhila Nida Ul 
Published: November 2020

Zat adiktif merupakan zat yang berdambak negatif bagi tubuh manusia apabila disalahgunakan. Di Kecamatan Blangkejeren Gayo Lues kegiatan penyalahgunaan zat adiktif penulis temukan beberapa kasus, tetapi perbuatan ini seringkali dianggap sepele oleh masyarakat karena tidak adanya hukum langsung yang mengaturnya. Pertanyaan penelitian dalam skripsi ini adalah bagaimana bentuk-bentuk penyalahgunaan zat adiktif yang dilakukan oleh anak-anak di Kecamatan Blangkejeren serta bagaimana sangsi yang dilaksanakan dalam rangka perlindungan terhadap anak di bawah umur. Untuk menjawab pertanyaan tersebut digunakan wawancara dan studi dokumentasi, sehingga data yang didapatkan bersifat kualitatif yang berbentuk deskriptif. Pendekatan yang digunakan adalah pendekatan hukum dan sosial dengan jenis studi kasus. Hasil penelitian ditemukan bahwa bentuk-bentuk penyalahgunaan zat adiktif yang dilakukan oleh anak-anak di Kecamatan Blangkejeren meliputi penyalahgunaan inhalansia, solven, minuman beralkohol dan rokok. Penyalahgunaan zat tersebut dilakukan sebagai permainan saja dan mereka tidak mengetahui dampak negatif dari zat tersebut serta konsekwensi hukumnya. Oleh karena itu, Penyalahgunaan zat tersebut termasuk pada kenakalan remaja yang bertentangan dengan norma adat dan hukum. Saksi yang diberikan kepada mereka dilakukan pembinaan serta dikembalikan kepada orang tua. Diharapkan kepada pihak terkait untuk sosialisasi dan pendidikan kepada masyarakat serta memberikan rehabilitasi terhadap anak penyalahgunaan zat adiktif. Kata kunci : Penyalahgunaan-Zat Adiktif-Anak di Bawah Umur
KEWAJIBAN MENYERTAKAN BUKTI PEMULA OLEH KORBAN DALAM PROSES PEMBUKTIAN KASUS PEMERKOSAAN (Studi Pasal 52 Qanun Aceh No.6/2014 Tentang Hukum Jinayat)
Authors: Andani Meri 
Published: November 2020

Terdapat permasalahan dalam hal korban pemerkosaan berkewajiban menyertakan alat bukti permulaan saat mengadukan suatu jarimah di dalam Pasal 52 ayat 1 dan 2 qanun hukum jinayat. Pasal ini mengindikasikan adanya beban ganda pada perempuan korban pemerkosaan karena mereka tidak hanya menjadi sasaran kejahatan yang mengerikan, namun juga harus menanggung beban menyertakan alat bukti permulaan. Jika bukti permulaan dibebankan kepada korban maka memberatkan dan melemahkan kaum perempuan sebagai korban untuk mengungkap pelaku pemerkosaan. Sehingga Semakin sulitnya membuktikan tindak pidana pemerkosaan maka akan semakin meningkatnya kasus pemerkosaan dan kekerasan seksual terhadap perempuan. Berdasarkan catatan komisi nasional anti kekerasan terhadap perempuan (KOMNAS perempuan), tampak kekerasan terhadap Perempuan di tahun 2018 dalam catatan tahunan (catahu) 2019 mengalami peningkatan yaitu sebesar 406.178 kasus naik sekitar 14% dibandingkan dengan tahun sebelumnya (catahu 2018) yaitu sebesar 348.446. Kata kunci: pembuktian-pemerkosaan-qanun jinayat.
Dossiê "Memória, verdade e justiça"
Authors: De Hollanda Cristina Buarque 
Published: November 2020

Ouso dizer que o leitor tem um tesouro nas mãos. O dossiê "Memória, verdade e justiça" reúne entrevistas com um punhado de personagens centrais do movimento homônimo. Ele - o movimento - nem sempre se chamou assim, nem tampouco tem ou teve um corpo bem delimitado de atores, ideias, estruturas e mecanismos. Sempre teve as fronteiras abertas, enlaçadas com outros movimentos, sem doutrina dura ou códigos de acesso. Organizou-se e desorganizou-se nas brechas e bordas do Estado, no seu coração e também no lado de fora dele. Seus integrantes - mesmo que não soubessem sê-lo - uniram-se nos afetos e desafetos que nascem das lutas e adversidades. Divergiram sobre um bocado de coisas, mas convergiram em torno da ideia de que a condição de olhar para frente é olhar para trás; de que do passado não se faz tábula rasa; de que é preciso lembrar e conhecer a ditadura para que "nunca mais se esqueça, nunca mais aconteça".