bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Case Study of Brass Foundry Workers’ Estimated Lead (Pb) Body Burden from Different Exposure Routes
Authors: Julander Anneli , Midander Klara , Garcia-Garcia Sandra , Vihlborg Per , Graff Pål 
Published: May 2020

Abstract

Objectives
The most pronounced occupational exposure routes for lead (Pb) are inhalation and gastrointestinal uptake mainly through hand-to-mouth behaviour. Skin absorption has been demonstrated for organic Pb compounds, but less is known about inorganic Pb species. Several legislative bodies in Europe are currently proposing lowering biological exposure limit values and air exposure limits due to new evidence on cardiovascular effects at very low blood Pb levels. In light of this, all exposure routes in occupational settings should be revisited to evaluate how to lower the overall exposure to Pb.

Methods
The aim of the study was to investigate the possible exposure routes in workers operating computer numerical control-machines in a brass foundry and specifically to understand if metal cutting fluids (MCFs) used by the workers could lead to skin absorption of Pb. The different bronze alloys at the facility may contain up to 20% Pb. After obtaining written informed consent from the workers (n = 7), blood, skin wipes, and personal air samples were collected. In addition, MCFs used on the day of exposure measurements were collected for in vitro skin absorption studies using stillborn piglet skin mounted in static Franz diffusion cells (n = 48). All samples were analysed for Pb content using inductively coupled plasma mass spectrometry.

Results
Pb air concentration (&lt;0.1–3.4 µg m−3) was well below the Swedish occupational exposure limit value. Blood Pb was in the range of &lt;0.72–33 µg dl−1, and Pb on skin surfaces, after performing normal work tasks during 2 h, was in the range of 0.2–48 µg cm−2. Using the MCFs in diffusion cells showed that skin absorption had occurred at very low doses, and that up to 10% of the Pb content was present in the skin after 24 h exposure. Using these results in the US EPA adult lead model, we could estimate a contribution to blood Pb from the three exposure routes; where hand-to-mouth behaviour yielded the highest contribution (16 µg Pb dl−1 blood), followed by skin absorption (3.3–6.3 µg Pb dl−1 blood) and inhalation (2.0 µg Pb dl−1 blood).

Conclusions
This case study shows that MCF may lead to skin absorption of inorganic Pb and contribute to a systemic dose (quasi-steady state). Furthermore, even though good hand hygienic measures were in place, the workers’ skin exposure to Pb is in all likelihood an important contributor in elevating blood Pb levels. Skin exposure should thus be monitored routinely in workers at facilities handling Pb, to help reducing unnecessary occupational exposure.
Does hunger lead to hybridization in a genus of sexually cannibalistic insects (Orthoptera: Prophalangopsidae)?
Authors: Dupuis Julian R , Judge Kevin A , Brunet Bryan M T , Ohlmann Chan Shawna , Sperling Felix A H 
Published: May 2020

AbstractAllochronic isolation can be a strong mechanism for reproductive isolation and speciation. However, imperfect allochrony and the expression of phenological plasticity can erode temporal barriers to gene flow and result in hybridization between divergent lineages. Here, we combine behavioural ecology and genomics to investigate this scenario in two closely related species of grigs in the genus Cyphoderris. These species exhibit a unique mating system whereby females feed on the fleshy hind wings of the male during copulation, and copulation with conspecific males is more likely in food-restricted females than in well-fed females. In western Canada, Cyphoderris buckelli and Cyphoderris monstrosa are sympatric but largely allochronically separated, with C. buckelli breeding earlier. However, their breeding seasons can overlap, leading to potential for older C. buckelli females to mate with young C. monstrosa males to obtain resources via sexual cannibalism. We used behavioural assays to test whether female feeding status affects the propensity for interspecific mating between C. buckelli females and C. monstrosa males. We then tested for hybridization and gene exchange in wild populations of both species, using morphology, mitochondrial DNA and genome-wide nuclear markers. We found that interspecific courtship and mating can occur, but the relationship between food restriction and increased propensity for hybridization was not significant. Although we observed intraspecific population genetic structure in both species, we found no signatures of hybridization in the morphological or genetic datasets, which suggests that postmating reproductive barriers might be preventing successful hybridization in the wild.
ACValidator: A novel assembly-based approach for in silico verification of circular RNAs
Authors: Sekar Shobana , Geiger Philipp , Adkins Jonathan , Tassone Erica , Serrano Geidy , Beach Thomas G , Liang Winnie S 
Published: May 2020

Abstract
Circular RNAs (circRNAs) are evolutionarily conserved RNA species that are formed when exons “back-splice” to each other. Current computational algorithms to detect these back-splicing junctions produce divergent results, and hence there is a need for a method to distinguish true-positive circRNAs. To this end, we developed Assembly based CircRNA Validator (ACValidator) for in silico verification of circRNAs. ACValidator extracts reads from a user-defined window on either side of a circRNA junction and assembles them to generate contigs. These contigs are aligned against the circRNA sequence to find contigs spanning the back-spliced junction. When evaluated on simulated datasets, ACValidator achieved over ∼80% sensitivity on datasets with an average of 10 circRNA-supporting reads and with read lengths of at least 100 bp. In experimental datasets, ACValidator produced higher verification percentages for samples treated with ribonuclease R compared to nontreated samples. Our workflow is applicable to non-polyA-selected RNAseq datasets and can also be used as a candidate selection strategy for prioritizing experimental validations. All workflow scripts are freely accessible on our GitHub page https://github.com/tgen/ACValidator along with detailed instructions to set up and run ACValidator.
Assessment of left atrial electro-mechanical delay to predict atrial fibrillation in hypertrophic cardiomyopathy
Authors: Tjahjadi Catherina , Hiemstra Yasmine L , van der Bijl Pieter , Pio Stephan M , Bootsma Marianne , Ajmone Marsan Nina , Delgado Victoria , Bax Jeroen J 
Published: May 2020

Abstract

Aims
Atrial fibrillation (AF) is frequently observed in hypertrophic cardiomyopathy (HCM) and is associated with poor clinical outcome. Total atrial conduction time, estimated by tissue Doppler imaging (TDI), the so-called PA-TDI duration, reflects the left atrial (LA) structural and electrical remodelling. The aim of this study was to evaluate the association between PA-TDI and new-onset AF in patients with HCM.

Methods and results
From a large cohort of patients with HCM, 208 patients (64% male, mean age 53 ± 14 years) without AF were selected. PA-TDI duration was measured from the onset P wave on electrocardiogram to the peak A′ wave of the lateral LA wall using TDI. The incidence of new-onset AF was 20% over a median follow-up of 7.3 (3.5–10.5) years. Patients with incident AF had longer PA-TDI duration when compared with patients without AF (133.7 ± 23.0 vs. 110.5 ± 30.0 ms, P &lt; 0.001). PA-TDI duration was independently associated with new-onset AF (hazard ratio: 1.03, 95% confidence interval: 1.01–1.05, P &lt; 0.001).

Conclusion
Prolonged PA-TDI duration was independently associated with new-onset AF in patients with HCM. This novel parameter could be useful to risk-stratify patients with HCM who are at risk of having AF.
Modeling Early Responses of Loblolly Pine Growth to Thinning in the Western Gulf Coastal Plain Region
Authors: Weng Y H , Grogan J , Coble D W 
Published: May 2020

Abstract
Growth response to thinning has long been a research topic of interest in forest science. This study presents the first 3–4 years of response of loblolly pine (Pinus taeda L.) growth to thinning at different intensities. Data were collected from the East Texas Pine Research Project’s region-wide loblolly pine thinning study, which covers a wide variety of stand conditions. Four treatments, light, moderate, and heavy thinning, respectively having 370, 555, and 740 residual trees per hectare after thinning, and an unthinned control, were included. Individual tree diameter at breast height (dbh) and total height were recorded annually for the first 3–4 years after thinning. Results indicate significant differences between treatments in dbh growth in each year after thinning, as well as for all years combined. Each thinning treatment had significantly greater dbh growth than the control in the first growing season with this positive response being more evident in the case of the heavier thinning or at the later years post-thinning. Conversely, the thinning effect on tree height growth was initially negligibly negative, then becoming positive after 2–4 years, with the heavier thinning becoming positive sooner. Tree size class, assigned based on prethinning dbh, had a significant effect on both dbh and height growth responses. Compared to the control, small trees had a greater response both in dbh and in height growth than the medium and large trees over the measurement period. At the stand level, the heavier thinning had significantly less stand basal area per hectare, but the difference in stand basal area per hectare between the thinned and the unthinned plots decreased with years post-thinning. Results from this study can improve our understanding in thinning effects and help forest managers make accurate decisions on silvicultural regimes.
Analysis of the 2017 June Maoxian landslide processes with force histories from seismological inversion and terrain features
Authors: Zhao J , Ouyang C J , Ni S D , Chu R S , Mangeney A 
Published: May 2020

SUMMARY
A devastating landslide occurred in Maoxian (China) on 2017 June 24, which generated strong signals that were recorded by a regional seismic network. We determined the landslide force history from long-period seismic waves and identified eight subevents. For each subevent, we obtained an independent force history and calculated its sliding path. The shape of the terrain before and after the landslide was found to play a critical role in the motion of the sliding mass. A combination of seismic and terrain data was used to discriminate between or relate the subevents to each other, and to locate the initiation point of each sliding path. We explain the Maoxian landslide dynamics as the combination of the rock collapse, centripetal acceleration of the sliding body, deceleration and acceleration once again after overcoming obstacles along the sliding path.
Decreased B Cells on Admission Associated With Prolonged Viral RNA Shedding From the Respiratory Tract in Coronavirus Disease 2019: A Case-Control Study
Authors: Hao Shaorui , Lian Jiangshan , Lu Yingfeng , Jia Hongyu , Hu Jianhua , Yu Guodong , Wang Xiaoyan , Xu Kaijin , Ni Qin , Li Yongtao , Liu Jun , Zhao Hong , Zhang Xuan , Yu Liang , Yu Xiaopeng , Xiang Dairong , Chen Yu , Zheng Shufa , Qiu Yunqing , Li Lanjuan , Liang Tingbo , Yang Yida , Sheng Jifang 
Published: May 2020

Abstract
The viral RNA shedding time (VST) for severe acute respiratory syndrome coronavirus 2 has not been well characterized. Clinical data were collected and compared between patients with short and long VSTs (in the lower and upper quartiles, respectively). The probability of recurrent positive reverse-transcription polymerase chain reaction results decreased sharply to 4.8% after 3 consecutive negative results. A series of ≥3 consecutive negative results was suitable as a criterion for the end of viral RNA shedding. The VST for shedding from the respiratory tract was significantly shorter in patients with normal B-cell counts on admission than in those with decreased B-cell counts (median [interquartile range], 11 [9–13] vs 16 [12–20] days, respectively; P = .001).
Identification and Characterization of Aminopeptidase-N as a Binding Protein for Cry3Aa in the Midgut of Monochamus alternatus (Coleoptera: Cerambycidae)
Authors: Guo Yajie , Carballar-Lejarazú Rebeca , Sheng Liangjing , Fang Yan , Wang Shaozhen , Liang Guanghong , Hu Xia , Wang Rong , Zhang Feiping , Wu Songqing 
Published: May 2020

AbstractBacillus thuringiensis Cry proteins have been widely used over the past decades for many different insect pests, which are safe for users and the environment. The coleopteran-specific Cry3Aa toxin from B. thuringiensis exhibits toxicity to the larvae of Monochamus alternatus. Receptors play a key role in the mechanisms underlying the toxic action of Cry. However, the binding receptor for Cry3Aa has yet to be identified in the midgut of M. alternatus larvae. Therefore, the aim of this study was to identify the receptor for Cry3Aa toxin in the brush border membrane vesicles (BBMVs) of M. alternatus larvae. Our results indicate that the Cry3Aa toxin binds to the BBMVs (Kd = 247 nM) of M. alternatus via a 107 kDa aminopeptidase N (APN) (Kd = 57 nM). In silico analysis of the APN protein predicted that an 18 amino acid sequence in the N-terminal acted as a signal peptide, and that the Asn residue, located at position 918 in the C-terminus is an anchored site for glycosyl phosphatidyl inositol. Further analysis showed that M. alternatus APN exhibits 75% homology to the APN from Anoplophora glabripenis. Our work, therefore, confirmed that APN, which is localized in the BBMVs in the midgut of M. alternatus larvae, acts as a binding protein for Cry3Aa toxins.
Validating scattering-induced (sub)millimetre disc polarization through the spectral index, wavelength-dependent polarization pattern, and polarization spectrum: the case of HD 163296
Authors: Lin Zhe-Yu Daniel , Li Zhi-Yun , Yang Haifeng , Looney Leslie , Stephens Ian , Hull Charles L H 
Published: May 2020

ABSTRACT
A number of young circumstellar discs show strikingly ordered (sub)millimetre polarization orientations along the minor axis, which is strong evidence for polarization due to scattering by ∼0.1 mm-sized grains. To test this mechanism further, we model the ALMA dust continuum and polarization data of HD 163296 using radmc-3d. We find that scattering by grains with a maximum size of 90  μm simultaneously reproduces the polarization observed at Band 7 and the unusually low spectral index (α ∼ 1.5) between Bands 7 and 6 in the optically thick inner disc as a result of more efficient scattering at the shorter wavelength. The low spectral index of ∼2.5 inferred for the optically thin gaps is reproduced by the same grains, as a result of telescope beam averaging of the gaps (with an intrinsic α ∼ 4) and their adjacent optically thick rings (where α ≲ 2). The tension between the grain sizes inferred from polarization and spectral index disappears because the low α values do not require large mm-sized grains. In addition, the polarization fraction has a unique azimuthal variation: higher along the major axis than the minor axis in the gaps, but vice versa in the rings. We find a rapidly declining polarization spectrum (with p ∝ λ−3 approximately) in the gaps, which becomes flattened or even inverted towards short wavelengths in the optically thick rings. These contrasting behaviours in the rings and gaps provide further tests for scattering-induced polarization via resolved multiwavelength observations.
Multiband observation of LIGO/Virgo binary black hole mergers in the gravitational-wave transient catalog GWTC-1
Authors: Liu Chang , Shao Lijing , Zhao Junjie , Gao Yong 
Published: May 2020

ABSTRACT
The Advanced LIGO and Virgo detectors opened a new era to study black holes (BHs) in our Universe. A population of stellar-mass binary black holes (BBHs) are discovered to be heavier than previously expected. These heavy BBHs provide us an opportunity to achieve multiband observation with ground-based and space-based gravitational-wave (GW) detectors. In this work, we use BBHs discovered by the LIGO/Virgo Collaboration as indubitable examples, and study in great detail the prospects for multiband observation with GW detectors in the near future. We apply the Fisher matrix to spinning, non-precessing inspiral-merger-ringdown waveforms, while taking the motion of space-based GW detectors fully into account. Our analysis shows that, detectors with decihertz sensitivity are expected to log stellar-mass BBH signals with very large signal-to-noise ratio and provide accurate parameter estimation, including the sky location and time to coalescence. Furthermore, the combination of multiple detectors will achieve unprecedented measurement of BBH properties. As an explicit example, we present the multiband sensitivity to the generic dipole radiation for BHs, which is vastly important for the equivalence principle in the foundation of gravitation, in particular for those theories that predict curvature-induced scalarization of BHs.
TopMatch-web: pairwise matching of large assemblies of protein and nucleic acid chains in 3D
Authors: Wiederstein Markus , Sippl Manfred J 
Published: May 2020

Abstract
Frequently, the complete functional units of biological molecules are assemblies of protein and nucleic acid chains. Stunning examples are the complex structures of ribosomes. Here, we present TopMatch-web, a computational tool for the study of the three-dimensional structure, function and evolution of such molecules. The unique feature of TopMatch is its ability to match the protein as well as nucleic acid chains of complete molecular assemblies simultaneously. The resulting structural alignments are visualized instantly using the high-performance molecular viewer NGL. We use the mitochondrial ribosomes of human and yeast as an example to demonstrate the capabilities of TopMatch-web. The service responds immediately, enabling the interactive study of many pairwise alignments of large molecular assemblies in a single session. TopMatch-web is freely accessible at https://topmatch.services.came.sbg.ac.at.
Is German Religionsverfassungsrecht under threat from the European Union?
Authors: Unruh Peter 
Published: May 2020

Abstract
Two recent judgments of the Court of Justice of the European Union (CJEU) raise fundamental questions about the relationship between European Union Law and German religious constitutional law. This article outlines the German constitutional context for the law of labour relations within religious associations before considering those judgments in detail. The article argues that in its approach to religious occupational requirements and loyalty obligations the case law of the CJEU risks bringing about a fundamental change in German religious constitutional law. This is in breach of the terms of membership of Germany in the European Union and contrary to European law itself.
UK Parties Abroad and Expatriate Voters: The Brexit Backlash
Authors: Collard Susan , Webb Paul 
Published: May 2020

Abstract
Recent developments in British politics have foregrounded two issues of particular importance to Britons living overseas: their voting rights in the UK and Brexit. In light of this, the number of British expatriates registering to vote has risen sharply and provided an incentive to develop UK parties abroad. We, therefore, set out the history and organisational structures of the major British parties abroad, and report the results of a unique online survey of British expatriate citizens, which tests whether ‘Votes for Life’ and Brexit have significantly impacted on their political preferences. We find that latter has done so, but the former has not. In view of this, the historically embedded expectations of a general expatriate preference for the Conservative Party no longer apply to those based in EU countries. In the wake of Brexit, this group appears to have swung decisively against the party and turned towards Labour and the Liberal Democrats. This suggests that the likelihood of the current Conservative administration introducing legislation to extend expatriate Britons’ voting rights, as pledged in the Queen’s Speech of December 2019, is remote, raising existential questions for the future of UK parties abroad.
What High-Income States Should Do to Address Industrial Antibiotic Pollution
Authors: Malmqvist Erik , Munthe Christian 
Published: May 2020

Abstract
Antibiotic resistance is widely recognized as a major threat to public health and healthcare systems worldwide. Recent research suggests that pollution from antibiotics manufacturing is an important driver of resistance development. Using Sweden as an example, this article considers how industrial antibiotic pollution might be addressed by public actors who are in a position to influence the distribution and use of antibiotics in high-income countries with publicly funded health systems. We identify a number of opportunities for these actors to incentivize industry to increase sustainability in antibiotics production. However, we also show that each alternative would create tensions with other significant policy goals, necessitating trade-offs. Since justifiable trade-offs require ethical consideration, we identify and explore the main underlying normative issues, namely, the weighing of local versus global health interests, the weighing of present versus future health interests, and the role of individualistic constraints on the pursuit of collective goals. Based on this analysis, we conclude that the actors have weighty principled reasons for prioritizing the goal of addressing pollution, but that translating this stance into concrete policy requires accommodating significant pragmatic challenges.
The relationship between glycated haemoglobin levels and the risk of giant cell arteritis – a case–control study
Authors: Mukhtyar Chetan , Myers Holly , Jones Colin , Dhatariya Ketan 
Published: May 2020

AbstractObjectivesThe EULAR core dataset for observational studies in GCA does not include glycated haemoglobin (HbA1c). A multivariable score to stratify the pre-test probability of GCA also does not include HbA1c. There have been contradictory reports about diabetes mellitus being a risk factor for GCA. We report the first study analysing the relationship of pre-diagnosis HbA1c with the risk of GCA.MethodsThis was a single-centre retrospective case–control study conducted in Norfolk, UK. All GCA cases were diagnosed with imaging or biopsy. Each case was assigned two age- and sex-matched controls. The primary outcome measure was the glycaemic status (HbA1c categorized into euglycaemia, pre-diabetes or diabetes mellitus) at diagnosis between cases and controls. The HbA1c was compared between two groups using the Mann–Whitney U test. The glycaemic categorization was compared using the χ2 test.ResultsOne hundred and twelve cases and 224 controls were included. The median (interquartile range) of HbA1c of cases and controls was 40 (37, 43) and 41 (39, 47) mmol/mol (P &lt; 0.001), respectively. Ten of 112 cases and 52 of 224 controls had diabetes mellitus. The χ2 test demonstrated a significant interaction between glycaemic state and GCA (P = 0.006). Individuals with diabetes mellitus had an odds ratio (95% CI) of 0.32 (0.13, 0.74) (P = 0.008) of having GCA compared with euglycaemic individuals.ConclusionHbA1c in the diabetic range reduces the probability of GCA. HbA1c should be considered in any multivariable score to calculate the risk of GCA, and in future development of diagnostic and classification criteria. There is a need for an epidemiological study looking at the possibility of a protective nature of diabetes mellitus against GCA or whether it is only a mimic.