bluuebunny/crossref_metadata_2025_split huggingface dataset:
Vascular smooth muscle cell phenotypic transition regulates gap junctions of cardiomyocyte
Authors: Zhou En , Zhang Tiantian , Bi Changlong , Wang Changqian , Zhang Zongqi 
Published: April 2020

AbstractAtrial fibrillation (AF) is one of the most prevalent arrhythmias. Myocardial sleeves of the pulmonary vein are critical in the occurrence of AF. Our study aims to investigate the effect of synthetic vascular smooth muscle cells (SMCs) on gap junction proteins in cardiomyocytes. (1) Extraction of vascular SMCs from the pulmonary veins of Norway rats. TGF-β1 was used to induce the vascular SMCs switching to the synthetic phenotype and 18-α-GA was used to inhibit gap junctions of SMCs. The contractile and synthetic phenotype vascular SMCs were cocultured with HL-1 cells; (2) Western blotting was used to detect the expression of Cx43, Cx40 and Cx45 in HL-1 cells, and RT-PCR to test microRNA 27b in vascular SMCs or in HL-1 cells; (3) Lucifer yellow dye transfer experiment was used to detect the function of gap junctions. (1) TGF- β1 induced the vascular SMCs switching to synthetic phenotype; (2) Cx43 was significantly increased, and Cx40 and Cx45 were decreased in HL-1 cocultured with synthetic SMCs; (3) The fluorescence intensity of Lucifer yellow was higher in HL-1 cocultured with synthetic SMCs than that in the cells cocultured with contractile SMCs, which was inhibited by18-α-GA; (4) the expression of microRNA 27b was increased in HL-1 cocultured with synthetic SMCs, which was attenuated markedly by 18-α-GA. (5) the expression of ZFHX3 was decreased in HL-1 cocultured with synthetic SMCs, which was reversed by 18-α-GA. The gap junction proteins of HL-1 were regulated by pulmonary venous SMCs undergoing phenotypic transition in this study, accompanied with the up-regulation of microRNA 27b and the down-regulation of ZFHX3 in HL-1 cells, which was associated with heterocellular gap junctions between HL-1 and pulmonary venous SMCs.
A compact wideband circularly polarized quadrifilar antenna with PIFA elements for UHF RFID readers
Authors: Lerkbangplad Chawanat , Namahoot Alongkorn , Akkaraekthalin Prayoot , Chalermwisutkul Suramate 
Published: April 2020

AbstractIn this paper, a compact circularly polarized quadrifilar antenna with planar inverted-F antenna (PIFA) elements is presented. The proposed antenna consists of four PIFA elements and a Wilkinson divider-based feed network fabricated on FR-4 substrate (ɛr = 4.4, loss tangent = 0.02, thickness = 1.6 mm). The total size of the antenna is 120 × 120 × 13.2 mm3. Impedance matching with a reflection coefficient <−15 dB and an axial ratio (AR) <3 dB are achieved over the global ultra-high frequency (UHF) radio frequency identification (RFID) frequency band and beyond. The realized gain ranges from 2.25 to 3.75 dBic within the frequency band of interest from 860 to 960 MHz with a directional radiation pattern. The proposed antenna is compact, low-cost and extremely wideband in terms of matching and AR compared to state-of-the-art UHF RFID reader antennas.
Comparisons Between Jupiter's X‐ray, UV and Radio Emissions and In‐Situ Solar Wind Measurements During 2007
Authors: Dunn W. R. , Gray R. , Wibisono A. D. , Lamy L. , Louis C. , Badman S. V. , Branduardi‐Raymont G. , Elsner R. , Gladstone G. R. , Ebert R. , Ford P. , Foster A. , Tao C. , Ray L. C. , Yao Z. , Rae I. J. , Bunce E. J. , Rodriguez P. , Jackman C. M. , Nicolaou G. , Clarke J. , Nichols J. , Elliott H. , Kraft R. 
Published: April 2020

AbstractWe compare Chandra and XMM‐Newton X‐ray observations of Jupiter during 2007 with a rich multi‐instrument data set including upstream in situ solar wind measurements from the New Horizons spacecraft, radio emissions from the Nançay Decametric Array and Wind/Waves, and ultraviolet (UV) observations from the Hubble Space Telescope. New Horizons data revealed two corotating interaction regions (CIRs) impacted Jupiter during these observations. Non‐Io decametric bursts and UV emissions brightened together and varied in phase with the CIRs. We characterize three types of X‐ray aurorae: hard X‐ray bremsstrahlung main emission, pulsed/flared soft X‐ray emissions, and a newly identified dim flickering (varying on short time scales, but quasi‐continuously present) aurora. For most observations, the X‐ray aurorae were dominated by pulsed/flaring emissions, with ion spectral lines that were best fit by iogenic plasma. However, the brightest X‐ray aurora was coincident with a magnetosphere expansion. For this observation, the aurorae were produced by both flickering emission and erratic pulses/flares. Auroral spectral models for this observation required the addition of solar wind ions to attain good fits, suggesting solar wind entry into the outer magnetosphere or directly into the pole for this particularly bright observation. X‐ray bremsstrahlung from high energy electrons was only bright for one observation, which was during a forward shock. This bremsstrahlung was spatially coincident with bright UV main emission (power > 1 TW) and X‐ray ion spectral line dusk emission, suggesting closening of upward and downward current systems during the shock. Otherwise, the bremsstrahlung was dim, and UV main emission power was also lower (<700 GW), suggesting their power scaled together.
The 3rd AGILE Terrestrial Gamma‐ray Flashes Catalog. Part II: Optimized Selection Criteria and Characteristics of the New Sample
Authors: Maiorana C. , Marisaldi M. , Lindanger A. , Østgaard N. , Ursi A. , Sarria D. , Galli M. , Labanti C. , Tavani M. , Pittori C. , Verrecchia F. 
Published: April 2020

AbstractWe present in this work the third catalog of terrestrial gamma‐ray flashes (TGFs) by the AGILE mission and the new search algorithm that was developed to produce it. We firstly introduce the new selection criteria, designed from the characteristics of WWLLN‐identified TGFs, and then applied on all data from March 2015 to September 2018. Association with sferics was performed by an independent search, described in a companion paper by Lindanger et al. (2020, https://doi.org/10.1029/2019JD031985). This search showed that many TGFs were not recognized by the existing selection algorithm, hence the need for this work. Several new selection criteria were tested and are compared in this paper. We then present the chosen selection criteria and the obtained sample, which includes 2,780 events and represents the most extensive TGF catalog available for the equatorial regions. Finally, we discuss the characteristics of this sample, including geographic distribution, intensity and duration, and seasonal variations.
ASPP1 deficiency promotes epithelial-mesenchymal transition, invasion and metastasis in colorectal cancer
Authors: Liu Dian , Ertay Ayse , Hill Charlotte , Zhou Yilu , Li Juanjuan , Zou Yanmei , Qiu Hong , Yuan Xianglin , Ewing Rob M. , Lu Xin , Xiong Hua , Wang Yihua 
Published: April 2020

AbstractThe apoptosis-stimulating protein of p53 (ASPP) family of proteins can regulate apoptosis by interacting with the p53 family and have been identified to play an important role in cancer progression. Previously, we have demonstrated that ASPP2 downregulation can promote invasion and migration by controlling β-catenin-dependent regulation of ZEB1, however, the role of ASPP1 in colorectal cancer (CRC) remains unclear. We analyzed data from The Cancer Genome Atlas (TCGA) and coupled this to in vitro experiments in CRC cell lines as well as to experimental pulmonary metastasis in vivo. Tissue microarrays of CRC patients with information of clinical-pathological parameters were also used to investigate the expression and function of ASPP1 in CRC. Here, we report that loss of ASPP1 is capable of enhancing migration and invasion in CRC, both in vivo and in vitro. We demonstrate that depletion of ASPP1 could activate expression of Snail2 via the NF-κB pathway and in turn, induce EMT; and this process is further exacerbated in RAS-mutated CRC. ASPP1 could be a prognostic factor in CRC, and the use of NF-κB inhibitors may provide new strategies for therapy against metastasis in ASPP1-depleted CRC patients.
Cholinergic midbrain afferents modulate striatal circuits and shape encoding of action strategies
Authors: Dautan Daniel , Huerta-Ocampo Icnelia , Gut Nadine K. , Valencia Miguel , Kondabolu Krishnakanth , Kim Yuwoong , Gerdjikov Todor V. , Mena-Segovia Juan 
Published: April 2020

AbstractAssimilation of novel strategies into a consolidated action repertoire is a crucial function for behavioral adaptation and cognitive flexibility. Acetylcholine in the striatum plays a pivotal role in such adaptation, and its release has been causally associated with the activity of cholinergic interneurons. Here we show that the midbrain, a previously unknown source of acetylcholine in the striatum, is a major contributor to cholinergic transmission in the striatal complex. Neurons of the pedunculopontine and laterodorsal tegmental nuclei synapse with striatal cholinergic interneurons and give rise to excitatory responses. Furthermore, they produce uniform inhibition of spiny projection neurons. Inhibition of acetylcholine release from midbrain terminals in the striatum impairs the association of contingencies and the formation of habits in an instrumental task, and mimics the effects observed following inhibition of acetylcholine release from striatal cholinergic interneurons. These results suggest the existence of two hierarchically-organized modes of cholinergic transmission in the striatum, where cholinergic interneurons are modulated by cholinergic neurons of the midbrain.
A meta-analysis of mannose-binding lectin gene polymorphisms with the risk of recurrent vulvovaginal infections
Authors: Kalia Namarta , Singh Jatinder , Rauniyar Akash Kumar , Kaur Manpreet 
Published: April 2020

AbstractThe genetic variants of Mannose-Binding Lectin, a vital component of innate immunity have been studied with acute/recurrent vaginal infections ((R)VVI) and presented inconclusive findings. Therefore, a systematic review and meta-analysis of published data were conducted to assess the possible role of these variations in (R)VVI. A comprehensive search was made using PubMed, Web of Science and Google scholar till June 18, 2019. A total of 12 studies met the specified criteria and were included in the analysis. Different comparisons were made on the basis of the outcome of interest that resulted in the filtering of studies for the pooled analysis to find an association using the standard genetic models. Odds ratio (OR) with 95% confidence interval (CI) was chosen as the effect measure for the data synthesis. The trim and fill technique was applied to adjust the publication bias. The meta-analysis revealed the significant association (p < 0.05) of rs1800450 polymorphism with RVVI risk (OR ≥ 3.5) in all the genetic models. The subgroup analysis identified the same association in Caucasian and Mixed ethnicity. Quantitative synthesis based on RVVC showed>3.5 fold risk of disease development accredited to rs1800450. A combined evaluation of Exon1 variants showed no association with (R)VVI. This meta-analysis suggests rs1800450 polymorphism as a genetic predisposing factor for RVVI, but to reinforce, further studies with a larger sample size are warranted.
Experimental comparison of clinically used ion beams for imaging applications using a range telescope
Authors: Kopp Benedikt , Meyer Sebastian , Gianoli Chiara , Magallanes Lorena , Voss Bernd , Brons Stephan , Parodi Katia 
Published: April 2020

Abstract
In particle therapy, the x-ray based treatment planning converting photon attenuation values to relative stopping power ratio (RSP) introduces clinically relevant range uncertainties. Recently, novel imaging technologies using transmission ion beams have been investigated to directly assess the water equivalent thickness (WET) of tissue, showing improved accuracy in RSP reconstruction, while potentially reducing the imaging dose. Due to their greater availability, protons have been mostly used for ion imaging. To this end, in this work, the influence of three ion species (protons, helium and carbon ions) on the image quality of radiographic WET retrieval has been explored with a dedicated experimental setup and compared to Monte Carlo (MC) simulations. Three phantom setups with different tissue interfaces and features have been irradiated with clinically validated proton, helium and carbon ion pencil beams under comparable imaging dose and beam settings at the Heidelberg Ion-Beam Therapy Center. Ion radiographies (iRADs) were acquired with an integration mode detector, that functions as a range telescope with 61 parallel plate ionization chambers. For comparison, experiments were reproduced in-silico with FLUKA MC simulations. Carbon ions provide iRADs with highest image quality in terms of normalized root mean square error, followed by helium ions and protons. All ions show similar capabilities of resolving WET for the considered phantoms, as shown by the similar average relative error < 3%. Besides for the slab phantom, MC simulations yielded better results than the experiment, indicating potential improvement of the experimental setup. Our results showed that the ability to resolve the WET is similar for all particles, intrinsically limited by the granularity of the detector system. While carbon ions are best suited for acquiring iRADs with the investigated integration mode detector, helium ions are put forward as a less technical challenging alternative.
Biodiesel production from Kesambi (Schleichera oleosa) oil using multi-walled carbon nanotubes supported zinc oxide as a solid acid catalyst
Authors: Asri N P , Yuniati Y , Hindarso H , Suprapto  , Yogaswara R R 
Published: April 2020

Abstract
The development of heterogeneous catalysts for biodiesel production became a hot topic among scientists because of its many advantages including easy separation and catalyst reusability. In this study, multi-walled carbon nanotubes supported zinc oxide (MWCNTsZ) as a solid acid catalyst had been successfully prepared via the stober-like process. There are several % loading of ZnO variations ranging from 10 – 35 % of catalyst weight. These catalysts were characterized by x-ray diffraction (XRD), BET surface area analysis, and scanning electron microscope with energy-dispersive x-ray spectroscopy (SEM-EDX) analysis. Transesterification of kesambi (Schleichera oleosa) oil also had been done to test the activity of the solid catalyst. The potential of this catalyst is shown from its large surface area with the maximum surface area until 409 m2/gram. However, the quantity of dispersed zinc oxide (ZnO) on multi-walled carbon nanotubes (MWCNTs) via that modified Stober preparation is quite small lower than 5% weight. This phenomenon is resulting in a low yield of biodiesel after 3 h of transesterification reaction. The highest yield of biodiesel is obtained with 20% weight of loading ZnO variable producing 13.82% yield of methyl ester.
Sonochemical synthesis of SrTiO3/TiO2 heterojunction material
Authors: Istighfarini V N , Aprilia S N L , Prasetyo A 
Published: April 2020

Abstract
A composite SrTiO3/TiO2 heterojunction photocatalyst was prepared by sonication methods. The heterojunction material SrTiO3/TiO2 was synthesized using an ultrasonic cleaning bath for 4 hours, by mixing SrTiO3 and TiO2 with variations in the comparison of mol 1:1, 1:2, 1:4 and 1:6. Characterization using XRD shows heterojunction material consisting of SrTiO3 and TiO2 anatase without any impurities, with the morphological surface observed using SEM indicating the agglomerated small particles. The bandgap of heterojunction material with a variation in mol SrTiO3: TiO2 of 1:1, 1:2, 1:4 and 1:6 are 3.11; 3.09; 3.07; and 3.07 eV respectively. The all of bandgap is lower than the SrTiO3 and TiO2 bandgap, i.e. 3.2 eV.
Isolation, identification, and bioactivity of steroids isolates from Hydrilla verticillata petroleum ether fraction
Authors: Fasya A G , Amalia S , Megawati D S , Salima F , Kusuma V A , Purwantoro B 
Published: April 2020

Abstract

Hydrilla verticillata contains some active compounds that potential as an antioxidant, antibacterial, anticancer, antimicrobial and antitumor. One of the active compounds in Hydrilla verticillata is steroids. This research aimed to isolate, to identify and to determine the toxicity and antioxidant activity of steroid compounds in petroleum ether (PE) fraction of Hydrilla verticillata. Hydrilla verticillata biomass powder was extracted by maceration using ethanol solvent. The ethanol extract was hydrolyzed with 2 N of hydrochloric acid and then partitioned with petroleum ether solvent. The steroid compounds from petroleum ether fraction were separated with Preparative Thin Layer Chromatography (TLC) and Column Chromatography. The steroid isolates were identified by UV-Vis and FTIR spectrophotometer. The toxicity level and antioxidant assay of steroid isolates were determined by BSLT and DPPH method, respectively. The result of the study showed that extraction through maceration produced 4.54% yield, whereas the product yield of the partition using petroleum ether was 65.41%. The steroid isolates from TLC and Column Chromatography separation has toxicity and antioxidant properties. The LC50 value of steroid TLC isolate was 1.41 and 12.2 ppm. The LC5o value of steroid Column Chromatography isolates (H2, H4 and H10) were 4.32, 8.24 and 10.35 ppm. The EC50 value of H2 Column Chromatography isolate was 23.00 ppm.
Anticancer activity in 2-methoxy-4-((4-methoxy-phenilimino)-methyl)-phenol compound on T47D breast cancer cells
Authors: Sukria L , Hayati E K , Hanapi A , Adawiyah R , Ningsih R 
Published: April 2020

Abstract
2-methoxy-4-((4-methoxyphenilimino)-methyl)-phenol compound is a Schiff base compound synthesized from vanillin and p-anisidin. The purpose of this research is to determine the stability of the 2-methoxy-4-((4-methoxyphenilimino) methyl) phenol compound and to test the anticancer activity of 2-methoxy-4-((4-methoxyphenilimino) methyl) phenol in inhibiting T47D breast cancer cells. The stability of the 2-methoxy-4-((4-methoxy-phenylimino)-methyl)- phenol compound was carried out by characterization using chemical tests, identification using FTIR and GC-MS. While the anticancer activity test of compound 2-methoxy-4-((4- methoxifenilimino) methyl) phenol using the MTT method. The results of re-characterization using chemical tests, identification of FTIR and KG-SM showed the compound was still stable. The IC50 value of compound 2-methoxy-4-((4-methoxyphenilimino) methyl) phenol was 353,038 μg/mL which showed weak activity in inhibiting T47D breast cancer cells.
Effect of doping Fe3+ and Cu2+ on the microstructure and electrical properties of cryptomelane-type MnO2 prepared by sol-gel method
Authors: Hastuti E , Agustin W R , Yuliana I 
Published: April 2020

Abstract
Manganese oxide (MnO2) is an alternative metal oxide material that has potential as an energy storage application. In this study, the cryptomelane-type MnO2 doped Fe3+ and Cu2+ were successfully prepared using the sol-gel method. Fumaric acid is added to the KMnO4 in aqueous solution and modified with FeCl3.6H2O and CuCl2.2H2O to form different properties. Phase transformation and lattice parameters of the products were characterized using X-ray diffractometry (XRD). SEM observed the morphology of the sample, and electrical properties were tested using RCL meter. The results showed that adding Fe3+ and Cu2+ provided a higher value of conductivity and capacity of MnO2.
Analysis of 1,4 naphthoquinone in the Indonesian medical plant from extract Eleutherine palmifolia (L.) Merr by UHPLC
Authors: Annisa R , Hendradi E , Yuwono M 
Published: April 2020

Abstract
Medicinal plants are widely used for the preparation of various pharmaceutical forms. Eleutherine palmifolia is a typical plant in Central Borneo in Indonesian. 1,4 naphthoquinone is a bioactive compound from E. palmifolia which has potential as a medicinal ingredient, one of which is anticancer. This study aimed to determine the levels of 1.4 naphthoquinone as a marker compound for the standardization of standardized herbal medicine (OHT) formulas of E. palmifolia extract. The separation was carried out with Thermo Fisher Scientific UHPLC Ultimate 3000 RS coupled with a diode array detector, and the C18 column was employed. The UV detection was performed at 254 nm, and the run time of 0.8 minutes. The mobile phase consists of an isocratic method, 95% methanol (A) and 0.5% chloroform (B). The results showed that 1,4 naphthoquinone was eluted at 3.260 min. The response of the standard 1,4 naphthoquinone linear in the concentration range of 3.0-21.0 μg/mL with r
2 = 0.9951. The accuracy of this method, which was 99.95% with RSD value ≤ 2%. UHPLC method was developed, which has been validated and shown applies to the determination and quantification of 1,4 naphthoquinone in E. palmifolia. Analysis of 1.4 naphthoquinone levels contained in E. palmifolia 12 μg/mL was 7.79 μg/mL ± 0.01.
Applying a stock-based model for estimating the amount of personal computer waste
Authors: Siringo R , Herdiyansyah H , Kusumastuti R D 
Published: April 2020

Abstract
The fourth industrial revolution has triggered an increase in the demand for information and communication technology equipment. Computers are useful in modern days and it is reflected in the ownership of these appliances which experienced steady growth in recent years. In 2017, about 20% of Indonesian households possess these devices. Undoubtedly, it will correspond to the escalating volume of computer waste. Furthermore, it raises concerns since valuable as well as toxic materials are embedded in e-waste. On the other hand, proper e-waste management is absent in the country. The current study aims to quantify the amount of computer waste generated in Indonesia by applying a stock-based model. The flow model is developed based on the secondary data of household ownership of computers, and the usage period is estimated using the lifetime distribution. The result indicates that computer lifespan in the country is 4.51 years. Besides, the 15 years prediction shows that in 2012 and 2026 the quantity of computer waste is about 1.16 million and 7.47 million respectively. It implies that the amount of e-waste will continue to increase. Therefore, it is imperative to design and implement sustainable e-waste management.
Construction analysis of the string motion model on Sasando musical instrument
Authors: Kusumastuti A , Brylliant D N , Hidayati N A 
Published: April 2020

Abstract
String is one of mathematical modelling object has been studied in the world. In this paper, researchers focus on the construction analysis of the string motion model on Sasando. The purpose of the research is to determine the form of the string motion on Sasando musical instrument and validating the model. Construction analysis was done by identifying problems, variables, parameters, energies, and the forces occur on the string of Sasando. Based on the identifying results, mathematical model that represents the problem of string motion on Sasando expressed as a second-order linear partial differential equation with u denote the vertical displacement experienced by the string at the point x at time t. The first step of the validation test is simulations of mathematical models using Matlab. The result obtained shows the oscillatory motions and the string move towards the equilibrium position. It shows that mathematical model of the string motion on Sasando reliable to real case. However, the result of first step validation test are not sufficient to determine validity of the model and next step validation test are needed to complete this research.