bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Convergence of Racial and Income Disparities in Health Insurance Coverage in the United States
Authors: Lee De-Chih , Liang Hailun , Shi Leiyu 
Published: December 2020

Abstract
ObjectiveThis study applies the vulnerability framework and examines the combined effect of race and income on health insurance coverage in the US. Results of the study could assist policymakers in targeting limited resources on subpopulations likely most in need of assistance for insurance coverage.Data sourcesThe household component of the US Medical Expenditure Panel Survey (MEPS-HC) in 2017 was used for the study.Study designLogistic regression models were used to estimate the associations between insurance coverage status and vulnerability measure, comparing insured with uninsured or partially insured, partially insured only, and uninsured only, respectively.Data collection/extraction methodsWe constructed a vulnerability measure that reflects the convergence of predisposing (race/ethnicity), enabling (income), and need (self-perceived health status) attributes of risk. Principal findingsWhile income was a significant predictor of health insurance coverage (a difference of 6.1%-7.2% between high- and low-income Americans), race/ethnicity was independently associated with lack of insurance. The combined effect of income and race on insurance coverage was devastating as low-income minorities with bad health had 66% less odds of being insured instead of uninsured or partially insured than high-income Whites with good health.ConclusionsPolicymakers should target insurance coverage for the most vulnerable subpopulation, i.e., those who have low income and are racial/ethnic minorities.
Iron Oxide Nanoparticle - Multiwall Carbon Nanotube Composite Materials for Batch or Chromatographic Biomolecule Separation
Authors: Schwaminger Sebastian Patrick , Brammen Markus , Zunhammer Florian , Däumler Nicklas , Fraga-García Paula , Berensmeier Sonja 
Published: December 2020

Abstract
Carbon-based materials are the spearhead of research in multiple fields of nanotechnology. Moreover, their role as stationary phase in chromatography is gaining relevance. We investigate a hybrid material consisting of multiwall carbon nanotubes (CNTs) and superparamagnetic iron oxide nanoparticles (SPIONs) towards its use as a mixed-mode chromatography material. The idea is to immobilize the ion exchange material iron oxide on CNTs as a stable matrix for chromatography processes without a 2 significant pressure drop. Iron oxide nanoparticles are synthesized and used to decorate the CNTs via a co-precipitation route. They bind to the walls of oxidized CNTs, thereby enabling to magnetically separate the hybrid material. This hybrid material is investigated with transmission electron microscopy, magnetometry, X-ray diffraction, X-ray photoelectron and Raman spectroscopy. Moreover, we determine its specific surface area and its wetting behavior. We also demonstrate its applicability as chromatography material for amino acid retention, describing the adsorption and desorption of different amino acids in a complex porous system surrounded by aqueous media. Thus, this material can be used as chromatographic matrix and as a magnetic batch adsorbent material due to the iron oxide nanoparticles. Our work contributes to current research on composite materials. Such materials are necessary for developing novel industrial applications or improving the performance of established processes.
High Sensitive Chiral Molecule Detector Based on The Amplified Lateral Shift in Kretschmann Configuration Involving Chiral TDBCs
Authors: Wang Song , Ye Qihui , Chen Xudong , Hu Yanzhu , Song Gang 
Published: December 2020

Abstract
We investigate a high sensitive chiral molecule detector based on Goos-Hanchen shift (S) in Kretschmann configuration involving chiral TDBCs. Fresnel equations and the stationary phase method are employed to calculate S. Due to the interaction between surface plasmon polaritons and chiral TDBCs, S with chiral TDBCs are amplified at near the resonant wavelengths of chiral TDBCs. Our calculation results show that although the difference between the resonant wavelengths of left and right TDBCs is 4.5nm, the difference of S with chiral TDBCs (ΔS) can reach to 400 times as the incident wavelength in certain conditions, which can be easily observed in experiments. There is an optimal thickness of the metal film to realize the largest difference of S between Kretschmann configurations with left TDBCs and right TDBCs. We also find that the positions of the largest S for the structures with left TDBCs and right TDBCs do not overlap. Furthermore, we discuss the oscillator strength f, which is mainly determined by TDBC concentration. We find that our proposed detector is quite sensitive with f. By changing f from 0.008 to 0.014 with the step of 0.002, the change of ΔS is no less than 5 times of the incident wavelength (2.9μm). Our proposed structure is very sensitive and has potential applications in experiments.
Transcriptome and Genome Evolution During HER2-amplified Breast Neoplasia
Authors: Lu Peipei , Foley Joseph , Zhu Chunfang , McNamara Katherine , Sirinukunwattana Korsuk , Vennam Sujay , Varma Sushama , Fehri Hamid , Srivast Arunima , Zhu Shirley , Rittscher Jens , Mallick Parag , Curtis Christina , West Robert 
Published: December 2020

Abstract
The acquisition of oncogenic drivers is a critical feature of cancer progression. For some carcinomas, it is clear that certain genetic drivers occur early in neoplasia and others late. Why these drivers are selected and how these changes alter the neoplasia’s fitness is less understood. Here we use spatially oriented genomic approaches to identify transcriptomic and genetic changes at the single duct level within precursor neoplasia associated with invasive breast cancer. We study HER2 amplification in ductal carcinoma in situ (DCIS) as an event that can be both quantified and spatially located via fluorescence in situ hybridization (FISH) and immunohistochemistry on fixed paraffin-embedded tissue. By combining the HER2-FISH with the laser capture microdissection (LCM) Smart-3SEQ method, we found that HER2 amplification in DCIS alters the transcriptomic profiles and increases diversity of copy number variations (CNVs). Particularly, interferon signaling pathway is activated by HER2 amplification in DCIS, which may provide a prolonged interferon signaling activation in HER2-positive breast cancer. Multiple subclones of HER2-amplified DCIS with distinct CNV profiles are observed, suggesting that multiple events occurred for the acquisition of HER2 amplification. Notably, DCIS acquires key transcriptomic changes and CNV events prior to HER2 amplification, suggesting that pre-amplified DCIS may create a cellular state primed to gain HER2 amplification for growth advantage.
Efficacy of Pediatric Integrative Manual Therapy In Cervical Movement Limitation In Infants With Positional Plagiocephaly: A Randomized Controlled Trial
Authors: Pastor-Pons Iñaki , García Cesar Hidalgo , Lucha-López María Orosia , Barrau-Lalmolda Marta , Rodes-Pastor Iñaki , Rodríguez-Fernández Ángel Luis , Tricás-Moreno José Miguel 
Published: December 2020

Abstract
Background: Positional plagiocephaly (PP) is a cranial deformation frequent amongst children and consisting in a flattened and asymmetrical head shape. PP is associated with excessive time in supine and with congenital muscular torticollis (CMT). Few studies have evaluated the efficiency of a manual therapy approach in PP. The purpose of this parallel randomized controlled trial is to compare the effectiveness of adding a manual therapy approach to a caregiver education program focusing on active rotation range of motion (AROM) and neuromotor development in a PP pediatric sample. Methods: 34 children with PP and less than 28 week old were randomly distributed into two groups. AROM and neuromotor development with Alberta Infant Motor Scale (AIMS) were measured. The evaluation was performed by an examiner, blinded to the randomization of the subjects. A pediatric integrative manual therapy (PIMT) group received 10-sessions involving manual therapy and a caregiver education program. The control group received the caregiver education program exclusively. To compare intervention effectiveness across the groups, improvement indexes of AROM and AIMS were calculated using the difference of the final measurement values minus the baseline measurement values. If the distribution was normal, the improvement indexes were compared using the Student t-test for independent samples; if not, the Mann-Whitney U test was used. The effect size of the interventions was calculated using Cohen's d. Results: All randomized subject were analysed. After the intervention, the PIMT group showed a significantly higher increase in rotation (29.68 ± 18.41º) than the control group (6.13 ± 17.69º) (p< 0.05). Both groups improved the neuromotor development but no statistical significant differences were found. No harm was reported during the study.Conclusion: The PIMT intervention program was more effective in increasing AROM than using only a caregiver education program.
Autism Spectrum Disorder and Anorexia Nervosa. Is There a Link? An Italian Experience
Authors: Pruccoli Jacopo , Solari Altea , Terenzi Letizia , Malaspina Elisabetta , Angotti Marida , Pignataro Veronica , Gualandi Paola , Sacrato Leonardo , Cordelli Duccio Maria , Franzoni Emilio , Parmeggiani Antonia 
Published: December 2020

Abstract
Background: Potential overlaps exist between psychopathological features of Anorexia Nervosa (AN) and Autism Spectrum Disorder (ASD). The impact of malnutrition on autistic traits in patients with AN should be considered. This study aims to investigate possible associations between Eating Disorders (ED) psychopathology, ASD traits and BMI in a group of young patients with AN, using the EDI-3 (Eating Disorder Inventory-3) test and gold-standard measures for ASD.Methods: Prospective study involving 23 inpatients admitted to an Italian Centre for paediatric ED. ASD traits and ED psychopathology were assessed administering ADOS-2 (Autism Diagnostic Observation Schedule-2), AQ (Autism Quotient) and EDI-3 tests. Both present and past autistic traits were investigated using different versions of AQ. Possible effect of BMI, Obsessive Compulsive Disorder (OCD) comorbidity and psychopharmacological treatments on results were evaluated with multiple linear regressions.Results: An ASD diagnosis was possible in 22% of patients. EDI-3 Interpersonal Problems (IPC), Affective Problems (APC), and Global Psychological Maladjustment (GPMC) subscales showed correlations with different ASD measures on ADOS-2 and AQ. Scores for ASD and their correlations with EDI-3 subscales were independent of BMI, age, OCD and psychopharmacological treatments.Conclusions: ASD traits presented high prevalence in a group of young inpatients with AN. These traits were significantly correlated to 3 specific EDI-3 subscales and independent of BMI. This is the first study to investigate the relationship between ASD traits as measured with gold-standard measures, EDI-3 scores, and BMI.
Knowledge of Neonatal Danger Signs and Associated Factors Among Mothers Who Gave Birth in the Past Six Months in Chole District, Arsi Zone, South East Ethiopia: Cross sectional Study
Authors: Getachew Tamirat , Assebe Tesfaye , Dheresa Merga , Eyeberu Addis , Gereziher Teklehaimanot 
Published: December 2020

Abstract
Background: Early detection of neonatal illness by mothers is an important step towards improving newborn survival. Even though mother’s knowledge of neonatal danger signs plays a critical role in reducing neonatal morbidity and mortality, studies on the area are limited and most of them are institution-based study which do not include rural mothers and mothers with home delivery. Therefore, the study aimed to assess knowledge of neonatal danger signs and associated factors among mother’s who gave birth in the past 6 months.Methods: Community-based cross-sectional study was conducted on 520 postnatal mothers by using multistage sampling method from March 1-15, 2019. The data were entered into Epi data version 3.1 and then exported into SPSS version 20 for analysis. Bivariate and multivariate analysis were used by using binary logistic regression to identify factors associated with mother’s knowledge of neonatal danger signs. Statistical significance was declared at p-value less than 0.05. Results: Mother’s level of knowledge on neonatal danger signs was found to be 50.2% (95%CI: 46.3%, 54.3%). Mother’s and husband’s secondary and above educational level [AOR= 2.15 95%CI: (1.11, 4.17)], and [AOR: 2.05, 95%CI: (1.07, 3.94) respectively, being in Urban [AOR=5.83, 95%CI: (2.77, 12.24)], had four or more antenatal visits [AOR: 2.10, 95%CI: (1.13, 3.90)], counseled during antenatal care [AOR= 4.33 95%CI: (1.88, 9.98)] and knowledge about essential newborn care [AOR=3.91 95%CI: (2.05, 7.48)] were significantly associated with mother’s knowledge of neonatal danger signs. Conclusion: The study revealed that mother’s level of knowledge towards neonatal danger signs was low. Mother’s educational status, husband’s educational status, place of residence, number of antenatal visits, counseled during antenatal visit and knowledge about essential newborn care were significantly associated.
Antiparasitic Effects of Plant Species from the Diet of Great Bustards
Authors: Bolívar Paula , Bautista Luis M. , Gómez María Teresa , Martínez Rafael A. , Andrés María Fe , Alonso Juan Carlos , Bravo Carolina , González-Coloma Azucena 
Published: December 2020

Abstract
Background: Diets combine food types according to some trade-offs, as for example maximising nutrients and minimising toxins. But some diets include elements because of their activity against the host parasites and other pathogens. This so-called medicinal role of food is under-reported in the literature, either because toxic elements in diets of livestock and wildlife are infrequent, or because their activity against parasites and pathogens has not been fully documented. We contribute to fill this knowledge gap by testing the activity of extracts and essential oils from Papaver rhoeas and Echium plantagineum against a selection of laboratory pathogens. These plants are strongly selected by great bustards Otis tarda during the mating season. Results: During this season we found a significantly higher frequency of P. rhoeas in male than in female faeces. The activity of different extracts of these plants against some laboratory models including a flagellated protozoan (Trichomonas gallinae), a nematode (Meloidogyne javanica) and a fungus (Aspergillus niger) was evaluated. We found activity against nematodes and trichomonads in non-polar and polar extracts of the aerial parts of P. rhoeas, especially the extracts of flowers and capsules, and E. plantagineum, especially the extracts of leaves and flowers.Conclusions: Both plants showed anti-parasitic activity, a result compatible with the hypothesis that great bustards eat plants for non-nutritional purposes, likely to assist them in coping with parasites and other pathogens, and P. rhoeas could be especially helpful for males during the mating season, when their immune system is weakened by the investment in secondary sexual characters and sexual display. The self-medication properties of plants and animals included in diets should be considered in studies of foraging behaviour, habitat selection, and even conservation biology of wildlife.
WITHDRAWN: Process Parameter Optimization in Fused Deposition Modeling (FDM) Using Response Surface Methodology (RSM)
Authors: Mustafa Muhammad Salman , Zafar Muhammad Qasim , Muneer Muhammad Arslan , Arif Muhammad , Siddiqui Farrukh Arsalan , Javed Hafiz Muhammad Asif 
Published: December 2020

Abstract
Fused Deposition Modeling (FDM) is a widely adopted additive manufacturing process to produce complex 3D structures and it is typically used in the fabrication of biodegradable materials e.g. PLA/PHA for biomedical applications. However, FDM as a fabrication process for such material needs to be optimized to enhance mechanical properties. In this study, dogbone and notched samples are printed with the FDM process to determine optimum values of printing parameters for superior mechanical properties. The effect of layer thickness, infill density, and print bed temperature on mechanical properties is investigated by applying response surface methodology (RSM). Optimum printing parameters are identified for tensile and impact strength and an empirical relation has been formulated with response surface methodology (RSM). Furthermore, the analysis of variance (ANOVA) was performed on the experimental results to determine the influence of the process parameters and their interactions. ANOVA results demonstrate that 44.7% infill density, 0.44 mm layer thickness, and 20C° printing temperatures are the optimum values of printing parameters owing to improved tensile and impact strength respectively. The experimental results were found in strong agreement with the predicted theoretical results.
Uterus Size May Affect Reproductive Outcome in Women with Adenomyosis After Gnrh- Agonist Pretreatment Undergoing Frozen Embryo Transfer Cycles: A Retrospective Cohort Study
Authors: Xiong Wei , Tang Ruiyi , Wu Peng , Sun Zhengyi , zhen jingran , Yu Qi 
Published: December 2020

Abstract
Background: GnRH-agonist is used to treat adenomyosis, but its efficacy in adenomyosis patients with uterine enlargement undergoing frozen embryo transfer (FET) is unclear. Methods：The retrospective cohort study comprised 112 adenomyosis patients with uterine enlargement undergoing the first FET circle. A long-term GnRH-a pretreatment was administered to 112 patients with uterine enlargement. These patients were divided into two groups according to the therapeutic effect: patients with a normal-size uterus after GnRH-a treatment (GN group) and patients with an enlarged uterus after GnRH-a treatment (GL group). Results：Not all patients can shrink their uterus to a satisfactory level. After receiving GnRH-a pretreatment, the uterus returned to normal size in 77% of patients (GN group), and 23% of patients had a persistently enlarged uterus (GL group). The pregnancy rate, clinical pregnancy rate, ongoing pregnancy rate, and live birth rate were significantly higher in the GN group than in the GL group. Controlling for the confounding factors, normal uterus size (odds ratio [OR] 4.50; P=0.03) and low body mass index (OR 3.13; P=0.03) affected the odds of achieving live birth. The cut-off value selected on the ROC curve of uterus volume after GnRH-a treatment for detecting live birth was 144.7Conclusions：GnRH-a pretreatment was associated with the regression of adenomyosis lesions and improved clinical pregnancy outcomes in the adenomyosis patients with uterine enlargement whose lesion are GnRH-a susceptible on FET cycles. However, about a quarter of patients may not be less responsive to GnRH-a and have poorer pregnancy outcomes, especially in overweight women.
SPAK-p38 MAPK Signal Pathway Modulates Claudin-18 and Barrier Function of Alveolar Epithelium after Hyperoxic Exposure
Authors: Shen Chih-Hao , Lin Jr-Yu , Lu Cheng-Yo , Yang Sung-Sen , Peng Chung-Kan , Huang Kun-Lun 
Published: December 2020

Abstract
Background: Hyperoxia downregulates the tight junction (TJ) proteins of the alveolar epithelium and leads to barrier dysfunction. Previous study has showed that STE20/SPS1-related proline/alanine-rich kinase (SPAK) interferes with the intestinal barrier function in mice. The aim of the present study is to explore the association between SPAK and barrier function in the alveolar epithelium after hyperoxic exposure. Methods: Hyperoxic acute lung injury (HALI) was induced by exposing mice to >99% oxygen for 64 hours. The mice were randomly allotted into four groups comprising two control groups and two hyperoxic groups with and without SPAK knockout. Mouse alveolar MLE-12 cells were cultured in control and hyperoxic conditions with or without SPAK knockdown. Transepithelial electric resistance and transwell monolayer permeability were measured for each group. In-cell western assay was used to screen the possible mechanism of p-SPAK being induced by hyperoxia.Results: Compared with the control group, SPAK knockout mice had a lower protein level in the bronchoalveolar lavage ﬂuid in HALI, which was correlated with a lower extent of TJ disruption according to transmission electron microscopy. Hyperoxia down-regulated claudin-18 in the alveolar epithelium, which was alleviated in SPAK knockout mice. In MLE-12 cells, hyperoxia up-regulated phosphorylated-SPAK by reactive oxygen species (ROS), which was inhibited by indomethacin. Compared with the control group, SPAK knockdown MLE-12 cells had higher transepithelial electrical resistance and lower transwell monolayer permeability after hyperoxic exposure. The expression of claudin-18 was suppressed by hyperoxia, and down-regulation of SPAK restored the expression of claudin-18. The process of SPAK suppressing the expression of claudin-18 and impairing the barrier function was mediated by p38 mitogen-activated protein kinase (MAPK).Conclusions: Hyperoxia up-regulates the SPAK-p38 MAPK signal pathway by ROS, which disrupts the TJ of the alveolar epithelium by suppressing the expression of claudin-18. Down-regulation of SPAK attenuates this process and protects the alveolar epithelium against the barrier dysfunction induced by hyperoxia.
GSK3β Rephosphorylation&nbsp;Rescues ALPL&nbsp;Deficiency Induced-impairment of Odontoblastic Differentiation of DPSCs
Authors: Zhang Liqiang , Zhao Jiangdong , Dong Jiayi , Liu Yuting , Xuan Kun , Liu Wenjia 
Published: December 2020

Abstract
Background: Premature exfoliation of deciduous teeth is a common manifestation in childhood patients with Hypophosphatasia (HPP), which is an autosomal inherited disease caused by ALPL mutations. Dysplasia of cementum, dentin and alveolar bone have been proposed to be the main reasons for the exfoliation of teeth, while the extraordinarily complex intracellular mechanisms remain elusive. Dental pulp stem cells (DPSCs) have been demonstrated to successfully regenerate functional pulp-dentin like tissue. Dental pulp cells derived from HPP patients impaired mineralization, however insight into the deeper mechanism is still unclear. Methods: The effects of ALPL on odontoblastic differentiation of DPSCs from HPP patient were assessed by Alizarin Red staining, immunofluorescent staining, Western blot and RT-PCR, and micro-CT assays. Results: Here, we found DPSCs from HPP patient exhibited low ALP activity and impaired odontoblastic differentiation. Meanwhile, we found that loss of function of ALPL reduced phosphorylation of GSK3β in DPSCs. While GSK3β rephosphorylation improved odontoblastic differentiation of HPP DPSCs with LiCl treatment. Finally, we demonstrated systemic LiCl injection ameliorated tooth associated defects in ALPL+/- mice by enhanced phosphorylation of GSK3β in teeth. Conclusions: Our study indicates that ALPL regulates odontoblastic differentiation of DPSCs, and provides useful information for understanding how ALPL deficiency leaded to tooth dysplasia and, ultimately, may inform efforts at improvement tooth defects in HPP patients.