bluuebunny/crossref_metadata_2025_split huggingface dataset:
Lipopolysaccharide-induced expansion of histidine decarboxylase-expressing Ly6G+ myeloid cells identified by exploiting histidine decarboxylase BAC-GFP transgenic mice
Authors: Takai Jun , Ohtsu Hiroshi , Sato Atsushi , Uemura Satoshi , Fujimura Tsutomu , Yamamoto Masayuki , Moriguchi Takashi 
Published: October 2019

AbstractHistamine is a biogenic amine that is chiefly produced in mast cells and basophils and elicits an allergic response upon stimulation. Histidine decarboxylase (HDC) is a unique enzyme that catalyzes the synthesis of histamine. Therefore, the spatiotemporally specificHdcgene expression profile could represent the localization of histamine-producing cells under various pathophysiological conditions. Although the bioactivity of histamine is well defined, the regulatory mechanism ofHdcgene expression and the distribution of histamine-producing cell populations in various disease contexts remains unexplored. To address these issues, we generated a histidine decarboxylase BAC (bacterial artificial chromosome) DNA-directed GFP reporter transgenic mouse employing a 293-kb BAC clone containing the entireHdcgene locus and extended flanking sequences (Hdc-GFP). We found that the GFP expression pattern in theHdc-GFP mice faithfully recapitulated that of conventional histamine-producing cells and that the GFP expression level mirrored the increasedHdcexpression in lipopolysaccharide (LPS)-induced septic lungs. Notably, a CD11b+Ly6G+Ly6Clowmyeloid cell population accumulated in the lung during sepsis, and most of these cells expressed high levels of GFP and indeed contain histamine. This study reveals the accumulation of a histamine-producing myeloid cell population during sepsis, which likely participates in the immune process of sepsis.
The roles of grouper clathrin light chains in regulating the infection of a novel marine DNA virus, Singapore grouper iridovirus
Authors: Wang Liqun , Li Qiang , Ni Songwei , Huang Youhua , Wei Jingguang , Liu Jiaxin , Yu Yepin , Wang Shaowen , Qin Qiwei 
Published: October 2019

AbstractClathrins, composed of clathrin heavy chains (CHCs) and clathrin light chains (CLCs), are usually hijacked by viruses for infection. However, the role of CLCs, especially in regulating fish virus infection, remains poorly understood. Here, two isoforms of CLCs were cloned from the red-spotted grouper (Epinephelus akaara) (EaCLCa and EaCLCb). Both EaCLC transcripts were expressed in all examined tissues, and the expression of EaCLCa was much higher than that of EaCLCb. Over-expressing EaCLCa-W119R mutant significantly reduced Singapore grouper iridovirus (SGIV) infectivity. However, no effect of EaCLCb-W122R on SGIV infection was observed. The detailed steps were further studied, mainly including virus attachment, entry and the following transport to early endosomes. EaCLCa-W119R mutant notably inhibited internalization of SGIV particles with no effect on SGIV attachment. Furthermore, EaCLCa-W119R mutant obviously impaired the delivery of SGIV to early endosomes after virus internalization. In addition, the EaCLCa-W119R mutant markedly reduced the colocalization of SGIV and actin. However, EaCLCb is not required for such events during SGIV infection. Taken together, these results demonstrate for the first time that EaCLCa and EaCLCb exerted different impacts on iridovirus infection, providing a better understanding of the mechanisms of SGIV infection and opportunities for the design of new antiviral strategies.
Cigarette smoke extract exacerbates hyperpermeability of cerebral endothelial cells after oxygen glucose deprivation and reoxygenation
Authors: Bernard Ashton , Ku Jacqueline M. , Vlahos Ross , Miller Alyson A. 
Published: October 2019

AbstractCigarette smoking is a risk factor for stroke and is linked to stroke severity. Previous studies have shown that cigarette smoke extract (CSE) triggers endothelial dysfunction in vitro by initiating oxidative stress and/or an inflammatory response. In addition, cerebral endothelial dysfunction (particularly at the level of the blood-brain barrier [BBB]) contributes to stroke pathogenesis. Therefore, we hypothesized that cigarette smoking may influence stroke, at least in part, by exacerbating ischaemia-induced BBB disruption. To test this, we examined the effect of CSE on the permeability of cerebral endothelial cells exposed to oxygen glucose deprivation and reoxygenation (OGD + RO). We found that the loss of BBB integrity following ischaemic/reperfusion-like conditions was significantly worsened by CSE. Despite this being associated with increased mRNA expression of Nox catalytic subunits, reactive oxygen species (ROS) levels were however markedly lower. Furthermore, this occurred in association with elevated expression of antioxidant enzymes (SOD1, SOD2, and Gpx-1), suggesting an antioxidant defence response. Lastly, we found that CSE significantly upregulated mRNA expression of cytokines (IL-6 and TGF-β). Collectively, these results show that acute exposure to CSE worsens BBB disruption caused by OGD + RO, however, this is not linked to elevated ROS levels but may involve inflammatory mechanisms.
Robot-assisted Nipple-sparing Mastectomy with Immediate Breast Reconstruction: An Initial Experience
Authors: Park Hyung Seok , Lee Jeea , Lee Dong Won , Song Seung Yong , Lew Dae Hyun , Kim Seung Il , Cho Young Up 
Published: October 2019

AbstractSeeking smaller and indistinct incisions, physicians have attempted endoscopic breast surgery in breast cancer patients. Unfortunately, there are some limitations in the range of movement and visualization of the operation field. Potentially addressing these limitations, we investigated the outcomes of gas and gasless robot-assisted nipple-sparing mastectomy (RANSM) with immediate breast reconstruction (IBR). Ten patients underwent 12 RANSM with IBR between November 2016 and April 2018. Patients with tumors measuring >5 cm in diameter, tumor invasion of the skin or nipple-areolar complex, proven metastatic lymph nodes, or planned radiotherapy were excluded. Age, breast weight, diagnosis, tumor size, hormone receptor status, and operation time were retrospectively collected. Postoperative outcomes including postoperative complications and final margin status of resected were analyzed. The median total operation time and console time were 351 min (267–480 min) and 51 min (18–143 min), respectively. The learning curve presented as a cumulative sum graph showed that the console time decreased and then stabilized at the eighth case. There was no open conversion or major postoperative complication. One patient had self-resolved partial nipple ischemia, and two patients experienced partial skin ischemia. We deemed that RANSM with IBR is safe and feasible for early breast cancer, benign disease of the breast, andBRCA 1/2mutation carriers. RANSM is an advanced surgical method with a short learning curve.
Uterine SOX17: a key player in human endometrial receptivity and embryo implantation
Authors: Kinnear Sophie , Salamonsen Lois A. , Francois Mathias , Harley Vincent , Evans Jemma 
Published: October 2019

AbstractThe yin and yang of female fertility is a complicated issue; large numbers of women/couples desire fertility and seek assisted reproduction intervention to achieve conception, while others seek to prevent pregnancy. Understanding specific molecules which control endometrial-embryo interactions is essential for both facilitating and preventing pregnancy. SOX17 has recently emerged as an important transcription factor involved in endometrial receptivity and embryo implantation. However, studies to date have examined mouse models of pregnancy which do not necessarily translate to the human. Demonstration of a role for ‘implantation factors’ in a human system is critical to provide a rationale for in depth clinical investigation and targeting of such factors. We demonstrate that SOX17is present within the receptive human endometrium and is up-regulated within human endometrial epithelial cells by combined estrogen & progesterone, the hormonal milieu during the receptive window. SOX17 localizes to the point of adhesive contact between human endometrial epithelial cells and a human ‘embryo mimic’ model (trophectodermal spheroid). Targeting SOX17 in endometrial epithelial cells using CRISPR/Cas9 knockdown or a SOX-F family inhibitor, MCC177, significantly inhibited adhesion of an trophectodermal spheroids to the epithelial cells thereby preventing ‘implantation’. These data confirm the important role of endometrial SOX17 in human endometrial receptivity and embryo implantation.
Association between Atrial Fibrillation, Myocardial Infarction, Heart Failure and Mortality in Patients with Nontuberculous Mycobacterial Infection: a nationwide population-based study
Authors: Park Chan Soon , Choi Eue-Keun , Kim Bongseong , Han Kyung-Do , Lee So-Ryoung , Cha Myung-Jin , Oh Seil 
Published: October 2019

AbstractNTM infection demonstrates an increasing incidence and prevalence. We studied the impact of NTM in cardiovascular events. Using the Korean nationwide database, we included newly diagnosed 1,730 NTM patients between 2005 and 2008 and followed up for new-onset atrial fibrillation (AF), myocardial infarction (MI), heart failure (HF), ischemic stroke (IS), and death. Covariates-matched non-NTM subjects (1:5, n = 8,650) were selected and analyzed. Also, NTM infection was classified into indolent or progressive NTM for risk stratification. During 4.16 ± 1.15 years of the follow-up period, AF, MI, HF, IS, and death were newly diagnosed in 87, 125, 121, 162, and 468 patients. In multivariate analysis, NTM group showed an increased risk of AF (hazard ratio [HR] 2.307, 95% confidence interval [CI] 1.560–3.412) and all-cause death (HR 1.751, 95% CI 1.412–2.172) compared to non-NTM subjects, whereas no significant difference in MI (HR 0.868, 95% CI 0.461–1.634), HF (HR 1.259, 95% CI 0.896–2.016), and IS (HR 1.429, 95% CI 0.981–2.080). After stratification, 1,730 NTM patients were stratified into 1,375 (79.5%) indolent NTM group and 355 (20.5%) progressive NTM group. Progressive NTM showed an increased risk of AF and mortality than indolent NTM group. Screening for AF and IS prevention would be appropriate in these high-risk patients.
Shape of a recoiling liquid filament
Authors: Contò Francesco Paolo , Marín Juan F. , Antkowiak Arnaud , Castrejón-Pita J. Rafael , Gordillo Leonardo 
Published: October 2019

AbstractWe study the capillary retraction of a Newtonian semi-infinite liquid filament through analytical methods. We derive a long-time asymptotic-state expansion for the filament profile using a one-dimensional free-surface slender cylindrical flow model based on the three-dimensional axisymmetric Navier-Stokes equations. The analysis identifies three distinct length and time scale regions in the retraction domain: a steady filament section, a growing spherical blob, and an intermediate matching zone. We show that liquid filaments naturally develop travelling capillary waves along their surface and a neck behind the blob. We analytically prove that the wavelength of the capillary waves is approximately 3.63 times the filament’s radius at the inviscid limit. Additionally, the waves’ asymptotic wavelength, decay length, and the minimum neck size are analysed in terms of the Ohnesorge number. Finally, our findings are compared with previous results from the literature and numerical simulations in Basilisk obtaining a good agreement. This analysis provides a full picture of the recoiling process going beyond the classic result of the velocity of retraction found by Taylor and Culick.
Morphology and development of the Portuguese man of war, Physalia physalis
Authors: Munro Catriona , Vue Zer , Behringer Richard R. , Dunn Casey W. 
Published: October 2019

AbstractThe Portuguese man of war, Physalia physalis, is one of the most conspicuous, but poorly understood members of the pleuston, a community of organisms that occupy a habitat at the sea-air interface. Physalia physalis is a siphonophore that uses a gas-filled float as a sail to catch the wind. The development, morphology, and colony organization of P. physalis is very different from all other siphonophores. Here, we look at live and fixed larval and juvenile specimens, and use optical projection tomography to build on existing knowledge about the morphology and development of this species. We also propose a framework for homologizing the axes with other siphonophores, and also suggest that the tentacle bearing zooids should be called tentacular palpons. Previous descriptions of P. physalis larvae, especially descriptions of budding order, were often framed with the mature colony in mind. However, we use the simpler organization of larvae and the juvenile specimens to inform our understanding of the morphology, budding order, and colony organization in the mature specimen. Finally, we review what is known about the ecology and lifecycle of P. physalis.
Phase Matching Quantum Key Distribution based on Single-Photon Entanglement
Authors: Li Wei , Wang Le , Zhao Shengmei 
Published: October 2019

AbstractTwo time-reversal quantum key distribution (QKD) schemes are the quantum entanglement based device-independent (DI)-QKD and measurement-device-independent (MDI)-QKD. The recently proposed twin field (TF)-QKD, also known as phase-matching (PM)-QKD, has improved the key rate bound from O(η) to O$$(\sqrt{{\boldsymbol{\eta }}})$$
(

η

)
with η the channel transmittance. In fact, TF-QKD is a kind of MDI-QKD but based on single-photon detection. In this paper, we propose a different PM-QKD based on single-photon entanglement, referred to as single-photon entanglement-based phase-matching (SEPM)-QKD, which can be viewed as a time-reversed version of the TF-QKD. Detection loopholes of the standard Bell test, which often occur in DI-QKD over long transmission distances, are not present in this protocol because the measurement settings and key information are the same quantity which is encoded in the local weak coherent state. We give a security proof of SEPM-QKD and demonstrate in theory that it is secure against all collective attacks and beam-splitting attacks. The simulation results show that the key rate enjoys a bound of O$$(\sqrt{{\boldsymbol{\eta }}})$$
(

η

)
with respect to the transmittance. SEPM-QKD not only helps us understand TF-QKD more deeply, but also hints at a feasible approach to eliminate detection loopholes in DI-QKD for long-distance communications.
Molecular Imaging of Endometriosis Tissues using Desorption Electrospray Ionization Mass Spectrometry
Authors: Feider Clara L. , Woody Spencer , Ledet Suzanne , Zhang Jialing , Sebastian Katherine , Breen Michael T. , Eberlin Livia S. 
Published: October 2019

AbstractEndometriosis is a pathologic condition affecting approximately 10% of women in their reproductive years. Characterized by abnormal growth of uterine endometrial tissue in other body areas, endometriosis can cause severe abdominal pain and/or infertility. Despite devastating consequences to patients’ quality of life, the causes of endometriosis are not fully understood and validated diagnostic markers for endometriosis have not been identified. Molecular analyses of ectopic and eutopic endometrial tissues could lead to enhanced understanding of the disease. Here, we apply desorption electrospray ionization (DESI) mass spectrometry (MS) imaging to chemically and spatially characterize the molecular profiles of 231 eutopic and ectopic endometrial tissues from 89 endometriosis patients. DESI-MS imaging allowed clear visualization of endometrial glandular and stromal regions within tissue samples. Statistical models built from DESI-MS imaging data allowed classification of endometriosis lesions with overall accuracies of 89.4%, 98.4%, and 98.8% on training, validation, and test sample sets, respectively. Further, molecular markers that are significantly altered in ectopic endometrial tissues when compared to eutopic tissues were identified, including fatty acids and glycerophosphoserines. Our study showcases the value of MS imaging to investigate the molecular composition of endometriosis lesions and pinpoints metabolic markers that may provide new knowledge on disease pathogenesis.
Diversity of PBI-DdeI satellite DNA in snakes correlates with rapid independent evolution and different functional roles
Authors: Thongchum Ratchaphol , Singchat Worapong , Laopichienpong Nararat , Tawichasri Panupong , Kraichak Ekaphan , Prakhongcheep Ornjira , Sillapaprayoon Siwapech , Muangmai Narongrit , Baicharoen Sudarath , Suntrarachun Sunutcha , Chanhome Lawan , Peyachoknagul Surin , Srikulnath Kornsorn 
Published: October 2019

AbstractTo better understand PBI-DdeI satellite DNA located in the centromeric region of python, molecular evolution analysis was conducted on 40 snake species. A ladder-like pattern of DNA bands with repetition of the 194–210 bp monomer was observed in 15 species using PCR. Molecular cloning was performed to obtain 97 AT-rich monomer sequences. Phylogenetic and network analyses showed three PBI-DdeI subfamilies with sequences grouped in species-specific clusters, suggesting rapid evolution. Slow evolution was found in eight species with shared PBI-DdeI sequences, suggesting recent species diversification, allowing PBI-DdeI no time to diverge, with limited homogenization and fixation processes. Quantitative real-time PCR showed large differences in copy number between Python bivittatus and other snakes, consistent with repeat scanning of whole genome sequences. Copy numbers were significantly higher in female Naja kaouthia than in males, concurring with chromosomal distribution of PBI-DdeI specifically localized to female W chromosomes. PBI-DdeI might act as an evolutionary driver with several repeats to promote W chromosome differentiation and heterochromatinization in N. kaouthia. Analysis revealed PBI-DdeI with a reduced copy number, compared to P. bivittatus, in most snakes studied, and it is possible that it subsequently dispersed and amplified on W chromosomes with different functional roles in N. kaouthia.
The abundance of Akkermansia muciniphila and its relationship with sulphated colonic mucins in health and ulcerative colitis
Authors: Earley Helen , Lennon Grainne , Balfe Áine , Coffey J. Calvin , Winter Desmond C. , O’Connell P. Ronan 
Published: October 2019

AbstractAkkermansia muciniphila utilises colonic mucin as its substrate. Abundance is reduced in ulcerative colitis (UC), as is the relative proportion of sulphated mucin in the mucus gel layer (MGL). It is unknown if these phenomena are related, however reduced sulphated mucins could contribute to reduced abundance, owing to a lack of substrate. The aim of this study was to quantify A. muciniphila within the MGL and to relate these findings with markers of inflammation and the relative proportion of sulphomucin present. Colonic biopsies and mucus brushings were obtained from 20 patients with active UC (AC), 14 with quiescent UC (QUC) and 20 healthy controls (HC). A. muciniphila abundance was determined by RT-PCR. High iron diamine alcian-blue staining was performed for histological analysis. Patients with AC had reduced abundance of A. muciniphila compared to HC and QUC. A positive association was found between A. muciniphila abundance and higher percentage of sulphated mucin (ρ 0.546, p = 0.000). Lower abundances of A. muciniphila correlated with higher inflammatory scores (ρ = 0.294 (p = 0.001)). This study confirms an inverse relationship between A. muciniphila and inflammation and a positive association between A. muciniphila abundance and percentage of sulfated mucin in the MGL.
Structural determination of Enzyme-Graphene Nanocomposite Sensor Material
Authors: Rai Durgesh K. , Gurusaran Manickam , Urban Volker , Aran Kiana , Ma Lulu , Li Pingzuo , Qian Shuo , Narayanan Tharangattu N. , Ajayan Pulickel M. , Liepmann Dorian , Sekar Kanagaraj , Álvarez-Cao María-Efigenia , Escuder-Rodríguez Juan-José , Cerdán María-Esperanza , González-Siso María-Isabel , Viswanathan Sowmya , Paulmurugan Ramasamy , Renugopalakrishnan Venkatesan 
Published: October 2019

AbstractState-of-the-art ultra-sensitive blood glucose-monitoring biosensors, based on glucose oxidase (GOx) covalently linked to a single layer graphene (SLG), will be a valuable next generation diagnostic tool for personal glycemic level management. We report here our observations of sensor matrix structure obtained using a multi-physics approach towards analysis of small-angle neutron scattering (SANS) on graphene-based biosensor functionalized with GOx under different pH conditions for various hierarchical GOx assemblies within SLG. We developed a methodology to separately extract the average shape of GOx molecules within the hierarchical assemblies. The modeling is able to resolve differences in the average GOx dimer structure and shows that treatment under different pH conditions lead to differences within the GOx at the dimer contact region with SLG. The coupling of different analysis methods and modeling approaches we developed in this study provides a universal approach to obtain detailed structural quantifications, for establishing robust structure-property relationships. This is an essential step to obtain an insight into the structure and function of the GOx-SLG interface for optimizing sensor performance.
Microarray profiling identifies extracellular circulating miRNAs dysregulated in cystic fibrosis
Authors: Ideozu Justin E. , Zhang Xi , Rangaraj Vittobai , McColley Susanna , Levy Hara 
Published: October 2019

AbstractExtracellular circulating miRNAs (ECmiRNAs) play a crucial role in cell-to-cell communication and serve as non-invasive biomarkers in a wide range of diseases, but their abundance and functional relevance in cystic fibrosis (CF) remain poorly understood. In this study, we employed microarray technology to identify aberrantly expressed plasma ECmiRNAs in CF and elucidate the functional relevance of their targets. Overall, we captured several ECmiRNAs abundantly expressed in CF. Expression levels of 11 ECmiRNAs differed significantly between CF and healthy control (HC) samples (FDR < 0.05, log2 FC≥2). Among these, 10 were overexpressed while only hsa-miR-598-3p was underexpressed in CF. The overexpressed miRNAs included three let-7 family members (hsa-let-7b-5p, hsa-let-7c-5p and hsa-let-7d-5p), three 103/107 family members (hsa-mir-103a-3p; hsa-mir-103b; hsa-mir-107), hsa-miR-486-5p, and other miRNAs. Using in silico methods, we identified 2,505 validated targets of the 11 differentially expressed miRNAs. Hsa-let-7b-5p was the most important hub in the network analysis. The top-ranked validated targets were involved in miRNA biogenesis and gene expression, including AGO1, DICER1, HMGA1, and MYC. The top pathways influenced by all targets were primarily signal transduction pathways associated with CF, including PI3K/Akt-, Wnt/β catenin-, glucocorticoid receptor-, and mTor signaling pathways. Our results suggest ECmiRNAs may be clinically relevant in CF and warrant further study.