bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comprehensive utilization of edible mushroom Auricularia auricula waste residue—Extraction, physicochemical properties of melanin and its antioxidant activity
Authors: Liu Xin , Hou Ruolin , Wang Danting , Mai Mengxian , Wu Xiaoping , Zheng Mingfeng , Fu Junsheng 
Published: October 2019

AbstractIn order to promote the comprehensive utilization of the Auricularia auricula waste residue, the extraction process and the physicochemical properties of melanin from A. auricula waste residue were studied. Furthermore, the chemical antioxidant activity of waste residue melanin and its protective effect on cell oxidative injury induced by H2O2 were investigated. The results indicated that the ultrasonic‐assisted extraction process could be used to extract the melanin from A. auricula waste residue. Melanin had a good solubility in alkali solution and exhibited a certain stability to thermal. There was no significant difference between A. auricula melanin control group and waste residue melanin on ABTS, DPPH, and hydroxyl radical scavenging activity. Waste residue melanin significantly inhibited the cell death caused by H2O2, and the cell viability was restored to 98.09 ± 5.97% when the melanin concentration was 1.6 mg/ml. Cell morphology observation confirmed that the melanin ameliorated the morphological changes of cells induced by oxidative stress.
Baseline functional connectivity may predict placebo responses to accelerated rTMS treatment in major depression
Authors: Wu Guo‐Rong , Wang Xiaowan , Baeken Chris 
Published: October 2019

AbstractAlthough in theory sham repetitive transcranial magnetic stimulation (rTMS) has no inherent therapeutic value, nonetheless, such placebo stimulations may have relevant therapeutic effects in clinically depressed patients. On the other hand, antidepressant responses to sham rTMS are quite heterogeneous across individuals and its neural underpinnings have not been explored yet. The current brain imaging study aims to detect baseline neural fingerprints resulting in clinically beneficial placebo rTMS treatment responses. We collected resting‐state functional magnetic resonance imaging data prior to a registered randomized clinical trial of accelerated placebo stimulation protocol in patients documented with treatment‐resistant depression (http://clinicaltrials.gov/show/NCT01832805). In addition to global brain connectivity and rostral anterior cingulate cortex (rACC) seed‐based functional connectivity (FC), elastic‐net regression and cross‐validation procedures were used to identify baseline intrinsic brain connectivity biomarkers for sham‐rTMS responses. Placebo responses to accelerated sham rTMS were correlated with baseline global brain connectivity in the rACC/ventral medial prefrontal cortex (vmPFC). Concerning the rACC seed‐based FC analysis, the placebo response was associated positively with the precuneus/posterior cingulate (PCun/PCC) cortex and negatively with the middle frontal gyrus. Our findings provide first brain imaging evidence for placebo responses to sham stimulation being predictable from rACC rsFC profiles, especially in brain areas implicated in (re)appraisal and self‐focus processes.
Investigation of charge interaction between fullerene derivatives and single‐walled carbon nanotubes
Authors: Delacou Clément , Jeon Il , Otsuka Keigo , Inoue Taiki , Anisimov Anton , Fujii Takenori , Kauppinen Esko I. , Maruyama Shigeo , Matsuo Yutaka 
Published: October 2019

AbstractThe charge interaction and corresponding doping effect between single‐walled carbon nanotubes (SWNTs) and various fullerene derivatives, namely, C60, phenyl‐C61‐butyric acid methyl ester (PC61BM), methano‐indenefullerene (MIF), 1′,1″,4′,4″‐tetrahydrodi[1,4]methanonaphthaleno[5,6]fullerene (ICBA), 1,4‐bis(dimethylphenylsilylmethyl)[60]fullerene (SIMEF‐1), and dimethyl(orthoanisyl) silylmethyl(dimethylphenylsilylmethyl)[60]fullerene (SIMEF‐2), are investigated. A variety of analytical techniques, including field‐effect transistors (FETs) made of horizontally aligned arrays of SWNTs, is used as a means of investigation. Data from different measurements have to be used to obtain a concrete evaluation for the fullerene‐applied SWNTs. The data collectively points toward the conclusion that fullerenes with high molecular orbital energy levels, namely, MIF, SIMEF‐1, SIMEF‐2, and PC61BM, induce p‐type doping, while fullerenes with low molecular orbital energy levels, namely, ICBA and C60, induce n‐type doping on the carbon nanotubes. Nevertheless, the SWNTs retained p‐type characteristics because n‐doping induced by the fullerenes are weak compared to the p‐doping of the water and oxygen on carbon nanotubes. This means that fullerene derivatives have the ability to fine‐tune the energy levels of carbon nanotubes, which can play a crucial role in carbon nanotube‐based electronics, such as solar cells, light‐emitting devices, and FETs.
The displacement study of 99mTc‐DTPA—Human serum albumin binding in presence of furosemide and metformin by using equilibrium dialysis and molecular docking
Authors: Chemlal Laila , Akachar Jihane , Makram Sanaa , Zoubir Brahim , Cherrah Yahia , Eljaoudi Rachid , Ibrahimi Azeddine , Faouzi Mly A. 
Published: October 2019

AbstractThe 99mTc‐DTPA (Technetium99m diethylenetriaminepentaacetic acid), is a radiopharmaceutical used in renal scintigraphy. The human serum albumin (HSA) binding site(s) for the 99mTc‐DTPA have never been characterized. This study will cover in vitro the binding rates of 99mTc‐DTPA on HSA and the 99mTc‐DTPA competition interactions with two drugs having known human serum albumin binding sites. Furosemide (FUR) and metformin (MET) were added to 99mTc‐DTPA solution (weight ratios 1/1 vol:vol) followed by the quantification of 99mTc‐DTPA binding rates to HSA (40 g/L) using equilibrium dialysis and the qualification of this binding using Molecular Modeling methods. The 99mTc‐DTPA binding rates to human serum albumin increased with the highest concentration. Both drugs FUR and MET displaced 99mTc‐DTPA binding. 99mTc‐DTPA could bind to human serum albumin in many locations in site I and I‐II, but strongly bound to site I through hydrogen bonds.
TRIM7 promotes proliferation and migration of vascular smooth muscle cells in atherosclerosis through activating c‐Jun/AP‐1
Authors: Ji Rongjing , Gu Yuanyuan , Zhang Jing , Gao Chuanyu , Gao Wanli , Zang Xiaobiao , Zhao Yonghui 
Published: October 2019

AbstractAtherosclerosis (AS), with associated risk of stroke or cerebrovascular disease, is one of the most common causes of death globally. It has been well established that tripartite motif‐containing protein 7 Tripartite Motif‐containing 7 (Trim7), as an E3 ubiquitin protein ligase, is involved in protein ubiquitination and thus regulating cellular proliferation. Moreover, TRIM7 is upregulated in advanced carotid AS. However, the detailed mechanism of TRIM7 on regulation of AS remains unclear. In the present study, we firstly discovered that TRIM7 expression was robustly induced in platelet‐derived growth factor type BB‐treated vascular smooth muscle cells (VSMCs) and human atherosclerotic plaques. Functional approaches established that knockdown of TRIM7 inhibited proliferation and migration of VSMCs, as well as arrested the cell cycle at G1‐S, thus suppressing AS progression. Our results also identified that c‐Jun/activator protein 1 (AP‐1) signaling pathway was activated by TRIM7. Moreover, gain‐ and loss‐of‐function studies revealed that TRIM7 could promote proliferation and migration of VSMCs via activation of c‐Jun/AP‐1 signaling pathway. Finally, by using atherogenic apolipoprotein E‐deficient (apoE–/–) C57BL/6 mice with high‐fat diet AS model, we demonstrated that interference of TRIM7 could effectively mitigate in vivo AS via inactivation of c‐Jun/AP‐1 signaling pathway. In general, activation of c‐Jun/AP‐1 signaling pathway via TRIM7 could be an important mechanism in AS progression, thus shedding light on the development of novel therapeutics to the treatment of the disease.
Epigenetic regulation of amniotic fluid mesenchymal stem cell differentiation to the mesodermal lineages at normal and fetus‐diseased gestation
Authors: Zentelytė Aistė , Gasiūnienė Monika , Treigytė Gražina , Baronaitė Sandra , Savickienė Jūratė , Borutinskaitė Veronika , Navakauskienė Rūta 
Published: October 2019

AbstractHuman mesenchymal stem cells isolated from amniotic fluid (AF‐MSCs) demonstrate the potency for self‐renewal and multidifferentiation, and can, therefore, be a potential alternative source of stem cells adapted for therapeutic purposes. The object of this study is to evaluate the efficacy of MSCs from AF when the pregnancy is normal or when the fetus is affected during pregnancy to differentiate into mesodermal lineage tissues and to elucidate epigenetic states responsible for terminal adipogenic and osteogenic differentiation. The morphology of AF‐MSCs from two cell sources and the expression of the cell surface‐specific (CD44, CD90, and CD105) markers and pluripotency (Oct4, Nanog, Sox2, and Rex1) genes were quite similar and underwent mesodermal lineage differentiation because this is shown by the typical cell morphology and of genes’ expression specific for adipogenic (peroxisome proliferator‐activated receptor‐ɣ, adiponectin) and osteoblastic (alkaline phosphatase, osteopontin, and osteocalcin) differentiation. Terminal lineage‐specific differentiation was related to differential expression of miR‐17, miR‐21, miR‐34a, and miR‐146a, decreased levels of acetylated H4 and H3K9, trimethylated H3K4 and H3K9, and the retention of H3K27me3 along with a reduction in the levels of HDAC1, DNMT1, and PRC1/2 proteins (BMI1/SUZ12). No significant distinction could be identified in the levels of expression of all epigenetic or pluripotency markers between undifferentiated MSCs isolated from AF of normal gestation and pregnancy where the fetus was damaged and between those differentiated toward adipocytes or osteoblasts. The expressional changes of those marks and microRNAs that occurred during terminal differentiation to mesodermal tissues indicate subtle epigenetic regulation in AF‐MSCs when the condition of the fetus is healthy normal or diseased. More detailed studies of epigenetic mechanisms may offer a better understanding of AF‐MSCs differentiation in fetus‐diseased conditions and their usage in an autologous therapeutic application and prenatal disease research.
Long noncoding RNA DLGAP1‐AS1 promotes cell proliferation in hepatocellular carcinoma via sequestering miR‐486‐5p
Authors: Peng Xiaochun , Wei Fengmei , Hu Xiaoli 
Published: October 2019

AbstractHepatocellular carcinoma (HCC) is most prevalent tumor in liver and one of the most fatal cancers in the world. Long noncoding RNAs (lncRNAs) have been accepted as important regulators in carcinomas. But there are still many lncRNAs including DLGAP1‐AS1 unannotated in HCC. First of all, GEPIA suggested that DLGAP1‐AS1 presented high expression in HCC tissue samples relative to the normal tissues. Besides, overexpression of DLGAP1‐AS1 was also proved in HCC cell lines. Moreover, DLGAP1‐AS1 knockdown efficiently suppressed cell proliferation in HCC. Interestingly, miR‐486‐5p was predicted and validated to interact with DLGAP1‐AS1, while the level of miR‐486‐5p was significantly increased In HCC after DLGAP1‐AS1 knockdown. Moreover, we uncovered that ectopic expression of miR‐486‐5p induced suppression on HCC cell proliferation and that miR‐486‐5p inhibition offset the effect of DLGAP1‐AS1 silence on HCC cell proliferation and apoptosis. Furthermore, H3F3B was identified as target of miR‐486‐5p and was therefore positively regulated by DLGAP1‐AS1 in HCC. Of note, H3F3B upregulation partly revived the declined cell proliferative capacity in response to DLGAP1‐AS1 knockdown. To conclude, DLGAP1‐AS1 exerted its oncogenic role in HCC via miR‐486‐5p/H3F3B axis. Our new findings provided novel theoretical basis for discovery of therapeutic targets of HCC.
A retrospective analysis of hyponatremia during terlipressin treatment in patients with esophageal or gastric variceal bleeding due to portal hypertension
Authors: Han Xv , Li Jia , Yang Ji‐Ming , Gao Min , Wang Lei 
Published: October 2019

Background and AimTo explore the risk factors of hyponatremia caused by terlipressin.MethodsForty‐four patients with acute variceal bleeding treated with terlipressin from December 2016 to December 2018 were analyzed.ResultsDuring the treatment, serum sodium levels decreased from 137.78 to 126.59 mmol/L (P < 0.05), with an average decrease of 11.19 mmol/L. The serum sodium level decreased by less than 5 mmol/L in 12 patients (27.27%), by 5–10 mmol/L in 13 patients (27.27%), and by more than 10 mmol/L in 19 patients (43.18%). The difference in baseline serum sodium levels was statistically significant (P < 0.05), and the differences in baseline total bilirubin levels, Child‐Pugh scores, and model for end‐stage liver disease scores were also significant. Logistic regression analysis suggested that the initial sodium level was an independent risk factor for the decrease in the serum sodium concentration caused by terlipressin.ConclusionThe incidence of hyponatremia is not low during treatment with terlipressin; a higher baseline serum sodium level is a risk factor for hyponatremia during treatment with terlipressin, and the mechanism may be related to endogenous vasopressin preconditioning.
An empirical prediction approach for seasonal fire risk in the boreal forests
Authors: Eden Jonathan M. , Krikken Folmer , Drobyshev Igor 
Published: October 2019

AbstractThe ability to predict forest fire risk at monthly, seasonal and above‐annual time scales is critical to mitigate its impacts, including fire‐driven dynamics of ecosystem and socio‐economic services. Fire is the primary driving factor of the ecosystem dynamics in the boreal forest, directly affecting global carbon balance and atmospheric concentrations of the trace gases including carbon dioxide. Resilience of the ocean–atmosphere system provides potential for advanced detection of upcoming fire season intensity. Here, we report on the development of a probabilistic empirical prediction system for forest fire risk on monthly‐to‐seasonal timescales across the circumboreal region. Quasi‐operational ensemble forecasts are generated for monthly drought code (MDC), an established indicator for seasonal fire activity in the Boreal biome based on monthly maximum temperature and precipitation values. Historical MDC forecasts are validated against observations, with good skill found across northern Eurasia and North America. In addition, we show that the MDC forecasts are an excellent indicator for satellite‐derived observations of burned area in large parts of the Boreal region. Our discussion considers the relative value of forecast information to a range of stakeholders when disseminated before and during the fire season. We also discuss the wider role of empirical predictions in benchmarking dynamical forecast systems and in conveying forecast information in a simple and digestible manner.
Sodium dodecyl sulfate free gel electrophoresis/electroelution sorting for peptide fractionation
Authors: Ramos Yassel , González Annia , Sosa‐Acosta Patricia , Perez‐Riverol Yasset , García Yairet , Castellanos‐Serra Lila , Gil Jeovanis , Sánchez Aniel , González Luis J. , Besada Vladimir 
Published: October 2019

AbstractShotgun proteomics based on peptide fractionation by using liquid chromatography has become the common procedure for proteomic studies, although in the very beginning of the field, protein separation by using electrophoresis was the main tool. Nonetheless, during the last two decades, the electrophoretic techniques for peptide mixtures fractionation have evolved as a result of relevant technological improvements. We also proposed the combination of sodium dodecyl sulfate polyacrylamide gel electrophoresis for protein fractionation and sodium dodecyl sulfate free polyacrylamide gel electrophoresis for peptide separation as a novel procedure for proteomic studies. Here, we present an optimized device for sodium dodecyl sulfate free polyacrylamide gel electrophoresis improving peptide recoveries respect to the established electrophoretic technique off gel electrophoresis meanwhile conserving the excellent resolution described for the former technique in slab gel based systems. The device simultaneously allows the separation and the collection of fractionated peptides in solution.
Preparation of porous magnetic molecularly imprinted polymers for fast and specifically extracting trace norfloxacin residue in pork liver
Authors: Zhang Yi , Xie Yang , Zhang Chungu , Wu Mingyu , Feng Shun 
Published: October 2019

AbstractHere, magnetic molecularly imprinted polymers were designed for norfloxacin via oil‐in‐water emulsifier‐free emulsion method. It was prepared by simply mixing norfloxacin, methacrylic acid‐co‐ethylene glycol dimethacrylate copolymer, and Fe3O4 together at room temperature. Characterized by multiple analytical tools, the particle size, pore size, pore volume, specific surface area, and saturation magnetization of the product were about 30 µm, 10–500 nm, 2.92 mL/g, 105.84 m2/g, and 3.052 emu/g, respectively. And the adsorption capacity was high at 27.04 mg/g towards norfloxacin. Combined with ultra high performance liquid chromatography, it was used to determine norfloxacin in real samples. Average recoveries were above 77.44% with relative standard deviations between 1.21 and 6.85% at three spiked levels (n = 3) for lake water and pork liver. The determination was achieved for the most complex biosample pork liver spiked with norfloxacin low to 30 ng/g, about 100 times less than the maximum residue limit regulated by Commission of the European Communities. All evidences demonstrated that the magnetic molecularly imprinted polymers can be used in practice for monitoring norfloxacin either in environmental water or meat product with high accuracy and reliability.
Signal enhancement in supercritical fluid chromatography‐diode‐array detection with multiple injection
Authors: Sun Mingzhe , Turner Charlotta , Sandahl Margareta 
Published: October 2019

AbstractTo circumvent the detrimental effects of large‐volume injection with fixed‐loop injector in modern supercritical fluid chromatography, the feasibility of performing multiple injection was investigated. By accumulating analytes from a certain number of continual small‐volume injections, compounds can be concentrated on the column head, and this leads to signal enhancement compared with a single injection. The signal to noise enhancement of different compounds appeared to be associated with their retention on different stationary phases and with type of sample diluent. The diethylamine column gave the best signal to noise enhancement when acetonitrile was used as sample diluent and the 2‐picolylamine column showed the best overall performance with water as the sample diluent. The advantage of multiple injection over one‐time large‐volume injection was proven with sulfanilamide, with both acetonitrile and water as sample diluents. The multiple injection approach exhibited comparable within‐ and between‐day precision of retention time and peak area with those of single injections. The potential of the multiple injection approach was demonstrated in the analysis of sulfanilamide‐spiked honey extract and diclofenac‐spiked ground water sample. The limitations of this approach were also discussed.
Base‐deactivated and alkaline‐resistant chromatographic stationary phase based on functionalized polymethylsilsesquioxane microspheres
Authors: Huo Zhixia , Chen Lei 
Published: October 2019

AbstractVinyl, chloropropyl, and mercaptopropyl functionalized particles were prepared by a two‐step acidic/alkaline catalyzed co‐hydrolysis/condensation of methyltrimethoxysilane with a different silane precursor that carries chemically reactive functional group including vinyl, chloropropyl, and mercaptopropyl, respectively. The morphology, pore structure, and functional groups of the synthesized packings were studied by SEM, nitrogen adsorption‐desorption measurements, and solid‐state 13C 29Si NMR spectroscopy, respectively. The particles show ordered sphere, narrow particle size distribution, and mesoporous structure. The carbon contents of the microspheres are in the range of 17–19%, comparable to those of octadecyl‐bonded silica packings. The three‐kind of microspheres were directly used as packing materials for high‐performance liquid chromatography without size classification. The chromatographic performance of the columns was evaluated and compared with a commercially available C18 phase. The results revealed that these columns possess typical reversed‐phase chromatographic properties with increased hydrophobicity than polymethylsilsesquioxane and symmetric peaks for basic compounds. They were applied to the simultaneous separation of combination bendazol hydrochlorothiazide capsules containing polar and basic drugs with peaks identified by tandem with mass spectrometry. In general, a novel method is provided for the synthesis of different methyltrimethoxysilane‐derived microspheres for high‐performance liquid chromatography, which are advantageous for separating basic compounds.
Is Frailty Associated With Worse Outcomes After Head and Neck Surgery? A Narrative Review
Authors: Fu Terence S. , Sklar Michael , Cohen Marc , de Almeida John R. , Sawka Anna M. , Alibhai Shabbir M.H. , Goldstein David P. 
Published: October 2019

ObjectiveFrailty has emerged as an important determinant of many health outcomes across various surgical specialties. We examined the published literature reporting on frailty as a predictor of perioperative outcomes in head and neck cancer (HNC) surgery.Study DesignNarrative review with limited electronic database search and cross‐referencing of included studies.MethodsPubMed was searched from inception until June 2019 to capture studies evaluating an association between frailty and perioperative outcomes among patients undergoing HNC surgery. Primary outcomes included mortality and morbidity, whereas secondary outcomes included in‐hospital cost, length of stay, readmission, and discharge disposition.ResultsWe identified nine series examining frailty as a predictor of outcomes in HNC. The majority of studies (77%) identified patients using a large population‐based database such as the National Surgical Quality Improvement Project or National Inpatient Sample. Frailty measures applied in the HNC surgery literature include the modified frailty index, Groningen Frailty Indicator, and John Hopkins Adjusted Clinical Groups frailty‐defining diagnosis indicator. Most studies demonstrated a significant association between frailty and perioperative outcomes, including mortality, perioperative complications, and Clavien‐Dindo grade IV complications. Furthermore, frailty was associated with greater length of hospital stay, readmission rate, and likelihood of discharge to short‐term or skilled nursing facilities.ConclusionThe current literature demonstrates the utility of frailty as a predictor of perioperative mortality and morbidity. Further research is needed to develop frailty screening measures in order to risk‐stratify patients and optimize modifiable factors preoperatively. Laryngoscope, 130:1436–1442, 2020