bluuebunny/crossref_metadata_2025_split huggingface dataset:
The prognostic values of CRP kinetics and initial apoA-I in patients with advanced NSCLC: a retrospective study
Authors: Zhou Danli , Cheng Ting , Zhang Jinping , Ling Jingjing , Hong Yuan , Ping Fengfeng , Wang Yan 
Published: July 2020

Abstract
Background: To analyse the value of C-reactive protein (CRP) kinetics and initial apolipoproteinA1 (apoA-I) level as prognostic parameters in advanced non-small Cell Lung Cancer (NSCLC). Methods : Within a retrospective single-center study, serum CRP and apoA-I level were measured at baseline and at the start of each platinum-based chemotherapy cycle for 125 NSCLC patients. Survival analysis, calculated by the Kaplan–Meier method and log-rank test, was used to assess the prognostic significance of Serum CRP and apoA-I level and both levels. The non-parametric Spearman rank correlation coefficient (rs) was used to measure the correlation between apoA-I and CRP. Results: Lung cancer-specific survival were significantly different across three CRP kinetics groups ( P <0.001), such significant differences were also seen between different apoA-I groups. In multivariate analysis, both CRP kinetics and apoA-I were independent significant factors for overall survival. Patients in non-normalized CRP group with lower apoA-I had a poor prognosis. Spearman’s rank correlation analysis revealed that the apoA-I level presented a negative correlation with the CRP level during treatment (r= -2.793, P <0.05). Conclusions: CRP kinetics and apoA-I may be useful to predict the prognosis of NSCLC patients treated with first-line platinum-based chemotherapy and facilitate individualized treatment.
IL-4/IL-13 Remodeling Pathway of Covid-19 Lung Injury
Authors: Paula Caroline Busatta Vaz de , Azevedo Marina Luise Viola , Nagashima Seigo , Martins Ana Paula Camargo , Malaquias Mineia Alessandra Scaranello , Ribeiro Anna Flavia Miggiolaro , Júnior Jarbas da Silva Motta , Avelino Gibran , Carmo Leticia Arianne Panini do , Carstens Lucas Baena , Noronha Lucia de 
Published: July 2020

Abstract
Background: The COVID-19 fatality rate is high when compared to the H1N1pdm09 (pandemic Influenza A virus H1N1 subtype) rate, and although both cause an aggravated inflammatory response, the differences in the mechanisms of both pandemic pneumonias need clarification.Objective: To analyze tissue expression of interleukins 4, 13, (IL-4, IL-13), transforming growth factor-beta (TGF-β), and the number of M2 macrophages (Sphingosine-1) in patients who died by COVID-19, comparing with cases of severe pneumopathy caused by H1N1pdm09, and a control group without lung injury.Methods: Six lung biopsy samples of patients who died of SARS-CoV-2 (COVID-19 group) were used and compared with ten lung samples of adults who died from a severe infection of H1N1pdm09 (H1N1 group) and eleven samples of patients who died from different causes without lung injury (CONTROL group). The expression of IL-4, IL-13, TGF-β, and M2 macrophages score (Sphingosine-1) were identified through immunohistochemistry (IHC).Results and conclusion: Significantly higher IL-4 tissue expression and Sphingosine-1 in M2 macrophages was observed in the COVID-19 group when compared to both the H1N1 and the CONTROL groups. Different mechanism of diffuse alveolar damage (DAD) in SARS-CoV-2 compared to H1N1pdm09 infections were observed. IL-4 expression and lung remodeling are phenomena observed in both SARS-COV-2 and H1N1pdm09. However, SARS-CoV-2 seems to promote lung damage through different mechanisms, such as the scarce participation Th1/Th17 response and the higher participation of the Th2. The understanding and management of the aggravated and ineffective immune response elicited by SARS-CoV-2 merits further clarification to improve treatments propose.
Academic performance and subtypes of psychological traits in undergraduate nursing students: A latent class analysis
Authors: Li Rui , Zhou Wanqiong , Wu Jing 
Published: July 2020

Abstract
Background: Undergraduate nursing students often encounter emotional problems due to academic stress. However, researchers have primarily focused on the impact of stressors, rather than understanding the subtypes of psychological state among students.Objectives: To identify subgroups of psychological traits among undergraduate nursing students using latent class analysis (LCA), and to examine the relationship between subgroups and exam failure risk.Design and Methods: A cross-sectional study was performed from May to July 2019. The Ruminative Responses Scale, Learning Burnout Questionnaire and Psychological Capital Scale were used to evaluate students’ psychological traits. Subjects were categorized into subgroups by latent class analysis. Cross-sectional comparisons and longitudinal regression analyses were subsequently performed.Settings and Participants: A total of 379 undergraduate nursing students from three medical colleges in Shanghai were investigated by cluster sampling method.Results: A total of four latent classes were identified based on the mean scores of rumination, learning burnout and psychological capital: Class 1 – General group (N=181, 47.76%); Class 2 – Negative psychologically dominated group (N=54, 14.25%); Class 3 – Positive psychologically dominated group (N=65, 17.15%); and Class 4 – Ambivalence psychological group (N=79, 20.84%). Students belonging into Class 1 and 2 displayed a higher rate of exam failure compared with those in Class 3 and 4 (p<0.05). Regression analysis showed that Class 1 (OR=2.108, 95%CI 1.240-4.118, p=0.006) and Class 2 (OR=2.694, 95%CI 1.229-6.293, p=0.016) independently predicted exam failure among undergraduate nursing students after adjusting for age, gender, grade and other academic related factors.Conclusions: Our findings provide practical implications for effective interventions that target each specific subgroup. Undergraduate nursing students with “Negative psychologically dominated group” were at a higher risk of exam failure. Urgent attention should be paid to address issues associated with psychological coping in students and their effect on learning outcomes.
SMPD3-mediated extracellular vesicle biogenesis inhibits oligodendroglioma growth
Authors: Balakrishnan Anjali , Adnani Lata , Chinchalongporn Vorapin , Vasan Lakshmy , Prokopchuk Oleksandr , Chen Myra , El-Sehemy Ahmed , Olender Thomas , Touahri Yacine , Ahmad Shiekh Tanveer , Islam Rehnuma , Sujanthan Sajeevan , Zinyk Dawn , Comanita Lacrimioara , Kan Boris , Fleming Taylor , Leong Hon , Morshead Cindi , Brand Marjorie , Wallace Valerie , Chan Jennifer , Schuurmans Carol 
Published: July 2020

Abstract
Oligodendrogliomas are lower-grade, slow-growing gliomas that are ultimately fatal. Although driver mutations are known, the mechanisms underlying their signature slow growth rates are poorly understood. We found evidence for intra-tumoral interactions between neoplastic and non-neoplastic cells in oligodendroglioma tissues. To further study these cell interactions, we used two patient-derived oligodendroglioma cell lines of lower and higher aggressivity. Both oligodendroglioma cell lines released extracellular vesicles that had cytotoxic effects on non-neoplastic and neoplastic cells, but each had distinct vesicular proteomes. Consistent with extracellular vesicles mediating growth inhibitory effects in oligodendrogliomas, higher expression levels of several extracellular vesicle biogenesis genes (SMPD3, TSG101, STAM1) correlates with longer survival in oligodendroglioma patients. Furthermore, SMPD3 overexpression slows oligodendroglioma cell growth in culture. Conversely, SMPD3 knockdown enhances oligodendroglioma proliferation in vitro, in murine xenografts, and in human cerebral organoid co-cultures. Oligodendroglioma-derived extracellular vesicles thus mediate tumor cell microenvironmental interactions that contribute to low aggressivity.
Deep-red circularly polarized luminescent C70 derivatives
Authors: Kano Haruka , Hayashi Hironobu , Matsuo Kyohei , Fujiki Michiya , Yamada Hiroko , Aratani Naoki 
Published: July 2020

Abstract
Optically active fullerenes (C76, C82, C84), including C60 and C70 derivatives carrying organic substituents, possess various potent applications because of unique spectroscopic, catalytic, and chiral recognition properties. However, their inherent photoexcited chirality has not yet been elucidated because of a very poor quantum yield of fluorescence (FL). With this background in mind, we synthesized new chiral C70 derivatives, X70A, solely by reacting bis-borylated xanthene with C70 in a one-step double addition reaction with 20% yield, followed by a successful optical resolution using a recyclable chiral-HPLC technique. The isolated X70A enantiomers were confirmed by their mirror-image circular dichroism spectra in the range of 300 nm and 700 nm. The enantiomeric pair of X70A in toluene revealed clearly mirror-image circularly polarized luminescence (CPL) spectra with a high |glum| value of 7.0 × 10–3 at 690 nm associated with FL lifetime of 0.99 ns. The deep-red CPL of X70A should provide new photofunctionality as chiral nanocarbon materials.
Approaching the necessity of pediatric palliative care in Colombia: a challenge for the health system.
Authors: Orrego Susana , Posada Karine , Librada Silvia , Lopera Juliana 
Published: July 2020

Abstract
Background: Pediatric Palliative Care (PPC) focuses on achieving quality of life and control the symptoms for children during the sickness process, also provide support to the family in order to relief suffering and give response to needs assessment. In Colombia, this subspecialty does not exist, as a result of not having clinic practice guides and information about the population who benefit under 18 years of age. This study is to approach the necessity of PPC in our country, defining a methodology to characterize and estimate prevalence of pediatric population who may need special care in order to develop an attention model. Methods: A retrospective descriptive study was conducted among 80,926 members between 0 and 18 years of age who has a private health insurance in Colombia between January 1, 2016 and December 31, 2018. Data was obtained from the insurance transactional database. Patients were grouped based on the Association for children with life threatening conditions and their families (ACT) of the Royal College of Pediatrics four classifications plus perinatal group. Results were processed and analyzed using descriptive statistical functions in Microsoft Excel. Results: 1,694 patients (2,09%) were eligible to enter a PPC program according to their IDC-10 diagnoses based on ACT plus group 5 classification. Approximately 54% were male, 57.97% were considered early childhood, 26.56% childhood and 15,47% adolescence. With regards to ACT classification and group 5; 55.55% of patients had diagnosis from group 5 (55.55%) followed by group 4 (23.2%) and group 1 non-cancer (9.68%). Being able to identify patients in need of PPC programs early may increase it being utilize at the time of diagnosis and not wait till the end of life. This might also help alleviate misconception of PPC only being offered during the end of life period. This study should help with the development of public and private politics aimed to promote the creation of PPC programs in developing countries.
Ethnobotanical Survey of Medicinal Plants Used in the Management of Diabetes in Ibadan North-East and Ibadan South-East, Oyo State, Nigeria
Authors: Ajayi Temitayo Olayemi , Attah Alfred F , Bafor Enitome E. , Tokede Goodness O. 
Published: July 2020

Abstract
Background: Diabetes mellitus, which is described to be a lifestyle disease, affects about 8.3% of the adult population of the world. Due to its alarming rate, it is one of the most common non-communicable diseases of current era. The burden of this disease is immense owing to transition in lifestyle and dietary habits, ageing of the population and urbanization in the setting of a genetically predisposed environment.Methodology: The study was aimed at documenting the knowledge of Traditional Medical Practitioners (TMPs) in Ibadan on ethnomedicinal plants used in the management of diabetes. A semi-structured questionnaire was used to obtain data from 100 TMPs and herb sellers within the study area. Data obtained was analysed using both descriptive statistics as well as quantitative ethnobotany including Use Mention Index, Use Value index and Fidelity level. Results:The total number of respondents from both study areas were 100, which were mainly females (94%) and most of them had no formal education. All respondents were Yoruba speaking. Results revealed 60 plants species belonging to 35 different families and 57 genera were cited by the respondents which they use in the management of diabetes. Hunteria umbellata is the most prominent plant species having the highest UMI reflecting it popularity and efficacy in the management of diabetes in both areas. Fabaceae (23%) has the highest number of plants species followed by Apocynaceae (17%), Annonaceae (11%), Curcurbitaceae (11%) and Liliaceae (8%). The leaves (25%) were the most commonly used plant parts for the management of diabetes followed by fruits (22%) and Bark (13%).in that order.Conclusion: The Southwest part of Nigeria is rich with unexplored plants useful in management of Diabetes and these documented traditional uses require scientific exploration and exploitation for drug discovery in diabetes therapy. Further studies are therefore encouraged in order to isolate, chemically elucidate and characterize the hypoglycaemic bioactive component which could serve as chemical lead for the development of novel antidiabetic drugs with desirable efficacy and safety profiles.
Rehmannia and Rhubarb Decoction Exerts Neuroprotective Effects on the Blood-brain barrier during Acute Intracerebral Hemorrhage by Downregulating the HMGB1/TLR4/MMP9 signaling pathway
Authors: Jiang Shan , Li Chun-Mei , Cai Ding-Fang , Zhang Jing-Si , Yu Xiao-Fei 
Published: July 2020

Abstract
Background

Blood-brain barrier (BBB) is a gate-keeping system with selective permeability that serves to protect the central nervous system. The underlying neuroprotective mechanism of the BBB during acute intracerebral hemorrhage (ICH) remains poorly understood. Rehmannia and rhubarb decoction (RRD) is a classic traditional Chinese medicine formula that has been extensively applied for hemorrhagic diseases in China. In the present study, the potential protective effects of RRD on the BBB during acute ICH and the underlying mechanism were investigated.
Methods

The ICH model was established by injection of autologous blood (100 µl) into spontaneously hypertensive rats, which were randomly divided into the following groups: i) Sham; ii) ICH; iii) RRD; iv) TAK-242; v) TAK-242 + RRD; vi) high mobility group box 1 protein (HMGB1) inhibitor ethyl pyruvate (EP); and vii) EP + RRD. Neurological deficits, pathological examination, brain water content, Evans blue(EB) extravasation, immunofluorescence staining and the expression levels of HMGB1, toll-like receptor 4 (TLR4), matrix metalloproteinase 9 (MMP-9), Claudin-5, Occludin and zona occludens − 1 (Zo-1) were subsequently examined in each group on day 3 following operation. In addition, MRI and transmission electron microscopy were also performed to observe the BBB structure.
Results

RRD treatment markedly improved neurological functions, reduced brain water content and Evans blue extravasation, ameliorated the disruption of BBB and downregulated HMGB1, TLR4 and MMP-9 expression whilst upregulating the expression of Claudin-5, Occludin and Zo-1.
Conclusion

These results demonstrate that RRD has a protective effect on the BBB in rats during ICH and this protective effect is related to the down-regulation of HMGB1/TLR4/MMP9 signaling pathway.
Identification of Hub Genes to Control Cancer Stem cell Characteristics in Colon Adenocarcinoma Through Bioinformatics Analysis and Validation by Western Blotting
Authors: Li Yu , Zhang Ye , Teng Yuhao , Wang Junyi , Zhang Jingyan , Ling Bofan , Shao Jie , Li Youran , Liu Baoxinzi 
Published: July 2020

Abstract
Background: Cancer stem cells (CSCs) are involved in the development of cancer. This study aimed to identify hallmark genes associated with the adjustment of CSC properties.Methods: The COAD data from The Cancer Genome Atlas(TCGA) database were assessed based on the mRNA stemness index (mRNAsi) and corrected mRNAsi. Then, both of them were analyzed with differentially expressed genes (DEGs). The gene modules were pinpointed through weighted gene co-expression network analysis (WGCNA). The genes of the most interest module were functionally annotated through Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG). The Search Tool for the Retrieval of Interacting Genes (STRING) was adopted to obtain protein-protein interaction (PPI) networks, and the hub genes were identified in the light of the Molecular Complex Detection (MCODE) and Cytoscape plugin cytoHubba. Upstream genes were analyzed via the DisNor. In addition, the associations between clinical variables and hub genes were estimated. The bioinformatic results were verified based on Gene Expression Profling Integrative Analysis (GEPIA), Oncomine and Gene Expression Omnibus (GEO). Finally, the protein expression of the hub genes was verified by western blotting.Results: Both the corrected mRNAsi and mRNAsi increased within COAD tissues relative to those within normal colon tissues, which showed a significantly decreasing trend in COAD as the clinical stage. GO indicated the biological processes, including muscle cell differentiation, multicellular organismal signaling, muscle system process and muscle contraction. KEGG revealed Tight junction, Dilated cardiomyopathy (DCM), Vascular smooth muscle contraction, and the cGMP PKG signal transduction pathway. The seven hub genes (ACTA2, CALD1, LMOD1, MYL9, MYLK, TAGLN and TPM2) were identified in the brown module. Most of them were associated with clinical stage; MYL9, TAGLN and TPM2 were associated with overall survival. TGFB1, SRF, ROCK1 and PRKCA were the upstream genes through the DisNor. In the Oncomine, GEO and GEPIA databases, the expression of seven hub genes were down-regulated. In TCGA and GEPIA databases, the tendency of the seven hub genes was decreased with the advance in stage. Conclusions: The hub genes identified in the present study meight play a vital role in the preservation of COAD stem cells.
Optimized Space Station CV Method and Its Differences from GNSS CV
Authors: Liu Yinhua , Li Xiaohui 
Published: July 2020

Abstract
There will be better atomic clock system and micro-wave time comparison link in the near earth space station, like Chinese Space Station and European ACES(Atomic Clock Ensemble in Space) system, than those in the GNSS(Global Navigation satellite System) satellites. Therefore, the space station common-view (CV) will realize more accurate time comparison than GNSS CV in theory. But due to the orbit characteristic of the space station, there are some limitations if traditional GNSS CV time comparison method is applied to the space station. In order to solve these problems, the GNSS CV method is optimized and the method that is appropriate for the space station is proposed. First, the basic CV principle is analyzed, and the delay items which are needed to be considered for GNSS and space station CV are compared and analyzed. Then, the differences between GNSS and space station CV are studied, and the influences of orbit error on these two CV methods are analyzed in detail. The GNSS CV method is optimized to be fit for the space station next. Finally, the performance of the optimized method is validated by simulated experiments. The simulation results show that the space station time comparison accuracy of several tens of picoseconds can be obtained by the optimized method. Furthermore, the problem of CV blind area is solved by the optimized method effectively.
REV3L Gene Variants rs1002481, rs462779, and rs465646 Lead to Increased Susceptibility Towards Non-Small Cell Lung Cancer in the Population of Jammu and Kashmir.
Authors: Jamwal Rajeshwar Singh , Mahajan Nikita , Bhat Gh. Rasool , Bhat Amrita , Sharma Bhanu , Shah Ruchi , Sharma Minerva , Verma Sonali , Bakshi Divya , Sharma Rahul , Abrol Deepak , Kumar Rakesh , Bhat Audesh 
Published: July 2020

Abstract
Background: Non-small cell lung cancer (NSCLC) is the most prevalent type of lung cancer accounting for 80–85% of all lung cancer cases. Various genetic studies have tried to reveal the association of REV3L (Protein reversion less 3-like) gene mutations with cancer, including lung cancer but no such study has been carried out in the population of Jammu and Kashmir (J&K).Methods: The selected REV3L variants were genotyped using the TaqMan allele discrimination assay in 550 subjects (203 NSCLC patients and 347 healthy controls). The association of variants was evaluated by logistic regression.Results: Out of the four REV3L variants genotyped, we found rs1002481, rs462779, and rs465646 significantly associated with NSCLC risk with an Odds Ratio (OR) of 3.5 (1.98–6.3 at 95% CI ), p value = 0.00002; OR = 4.4 (1.8–10.4 at 95% CI ), p value = 0.00075; and OR = 2.4 (1.47–4.008 at 95% CI ), p value = 0.00053, respectively.Conclusion: Our data suggest a strong association of variants rs1002481, rs462779, rs465646 with NSCLC. This association is indicative of a potential role of mutations in the REV3L gene as risk factor for the development of NSCLC in the studied population. Although a first report from any Indian population, these variants have been previously reported to be associated with lung and colorectal cancers in different world populations. These data along with our data supports the notation that these variants can be used as potential prognostic biomarker.
Rac1 signaling regulates LPS-induced mouse mastitis via inhibition of NLRP3, NF-κB and MAPK signaling pathways
Authors: Wang Chaoqun , Jiang Aimin , Jingjing Wang , Liu Xiao , Zhen Han , Zhengkai Wei , Yang Zhengtao 
Published: July 2020

Abstract
Background

Bovine mastitis characterized by mammary gland inflammatory responses, is the most frequent and costly diseases in dairy cattle. Researchers have been seeking effective treatments for this disease, but still not very successful. Ras-related C3 botulinum toxin substrate 1 (Rac1) is implicated in various cellular functions, including apoptosis, ROS production and inflammatory responses, and presents an attractive therapeutic target for many diseases. However, the effects of Rac1 signaling on mastitis remain unclear. The aim of this study is to investigate the effects of Rac1 signaling on mastitis via inhibition by Rac1 specific inhibitor NSC23766 based on a murine model of lipoplysaccharide (LPS)-induced mastitis.
Methods

Murine mastitis model was established by perfusion of LPS, hematoxylin-eosin (H & E) staining was employed to explore the mechanisms in mouse model, qRT- PCR analysis, Western blotting analysis.
Results

The results revealed that NSC23766 significantly decreased the damage of mammary gland by LPS, reduced myeloperoxidase activities, and the productions of IL-1β, IL-6, TNF-α and MCP-1 gene expression in the mammary glands with LPS perfusion. Moreover, western blot analysis showed that NSC23766 inhibited the phosphoryation of p65, IκBα, ERK, and p38, and suppressed the expression of NLRP3.
Conclusion

These findings suggested that administration of NSC23766 prevented the development of mastitis by inhibiting NLRP3, mitogen-activated protein kinase (MAPK) and nuclear factor-κB (NF-κB) signaling pathways. Accordingly, this study may provide research basis for the development of new drugs against mastitis, and NSC23766 might be a potential therapeutic drugs for mastitis.