bluuebunny/crossref_metadata_2025_split huggingface dataset:
Abstract B84: In vitro and in vivo characterization of tumor-educated human monocytic myeloid-derived suppressor cells
Authors: Singh Latika , Stivers Peter , Palmieri Anthony , Zhang Mark , Joyce-Shaikh Barbara , Zhang-Hoover Jie , Qu Yujie , Byford Alan , Meehl Michael , Brandish Philip 
Published: March 2020

Abstract
Tumor-driven immune suppression can arise from the recruitment and activation of immunosuppressive cell populations, including myeloid-derived suppressor cells (MDSCs). MDSCs are immature myeloid cells that accumulate in tumor microenvironment and dampen the antitumor immune response. However, the mechanisms of MDSC induction remain poorly understood. A greater understanding of their induction and characterization will enable the development of treatments with the potential to combat tumor-induced immune suppression. We characterized the phenotype and function of human tumor cell line (SKMEL5) educated monocytic MDSCs (M-MDSC) in vitro and in vivo. M-MDSCs were generated via coculture of human PBMCs with SKMEL5 tumor cells. The induced suppressive cells were assessed with respect to their cell surface phenotype, cytokine secretion profile, and T-cell suppression (measured by 3H-Thymidine proliferation assay and IFNy secretion). We further identified molecular markers that distinguish M-MDSCs from naive CD33 myeloid cells. In vivo, humanized mice bearing SKMEL5 tumors were characterized for infiltration of immune cells. Results from in vitro model indicated that tumor cell line educated M-MDSCs expressed low levels of HLA-DR and CD86, high levels of CD33, CD11b and suppressed both CD8+T-cell proliferation and IFNy secretion. Comparing M-MDSC and naive CD33 myeloid gene expression revealed upregulation of IL-10, PDL1/2, and VEGFR1 in M-MDSCs. Furthermore, M-MDSC expressed high levels of two new drug targets, immunoglobulin-like transcript 3 (ILT3, also known as LILRB4) and immunoglobulin-like transcript 4 (ILT4 also known as LILRB2). We investigated how ILT-targeting could modulate M-MDSC cell function. In addition, In vivo results from humanized mice bearing SKMEL5 tumors demonstrated expansion of multiple subtypes of tumor-associated myeloid cells with increased ILT3 and ILT4 expression. These human MDSC models provide a platform for building biologic understanding of emerging myeloid targets expressed in pembrolizumab nonresponders and tumors rich in myeloid-suppressive cells.
Citation Format: Latika Singh, Peter Stivers, Anthony Palmieri, Mark Zhang, Barbara Joyce-Shaikh, Jie Zhang-Hoover, Yujie Qu, Alan Byford, Michael Meehl, Philip Brandish. In vitro and in vivo characterization of tumor-educated human monocytic myeloid-derived suppressor cells [abstract]. In: Proceedings of the AACR Special Conference on Tumor Immunology and Immunotherapy; 2019 Nov 17-20; Boston, MA. Philadelphia (PA): AACR; Cancer Immunol Res 2020;8(3 Suppl):Abstract nr B84.
Abstract B88: GPNMB expression modulates the tumor immune microenvironment in mouse models of breast cancer
Authors: Annis Matthew G. , Rose April A.N. , Ahn Ryuhjin , Hsu Brian E. , Ursini-Siegel Josie , Siegel Peter M. 
Published: March 2020

Abstract
The role of glycoprotein-NMB (GPNMB) in the immune system is varied. GPNMB expression in macrophages and dendritic cells promotes innate immune responses, whereas GPNMB-expressing myeloid-derived suppressor (MDSC) cells suppress adaptive immune responses (T-cell function). While functional roles for GPNMB expressed within cells of the immune system are emerging, the influence of tumor-derived GPNMB expression on the immune landscape within mammary tumors has not been well characterized. To investigate this further, we have generated two GPNMB-deficient breast cancer cell populations (Lung Metastatic-4T1 [LM-4T1] and E0771 cells) to determine the influence of GPNMB on the tumor immune microenvironment (TME). While loss of GPNMB significantly impaired tumor growth of both these breast cancer models in syngeneic mice, this difference was not apparent with LM-4T1 injected in athymic nude mice. These observations suggest GPNMB may promote tumor growth by modulating the T-cell function. We have used traditional immunohistochemical analyses to broadly characterize the T cells present in the TME of E0771 and LM-4T1 breast tumors, which express or lack GPNMB. We have observed a significant increase in both CD8+ and CD4+ immune cells in GPNMB-deficient LM-4T1 and E0771 tumors compared to parental cells at an experimental endpoint of matched tumor volumes. To determine the temporal response of the immune system to LM-4T1 cells, we have isolated early developing lesions and stained for CD8, CD4, Fox3p, and granzyme B positive cells. We observed a significant increase in CD4+ and a trend for elevated granzyme B+ cells at early timepoints in GPNMB-deficient tumors compared to LM-4T1 parental cells. Taken together, these results suggest GPNMB suppresses an early recruitment of CD4+ cells where, in the absence of GPNMB, this would result in elevated CD8+ and CD4+ cells in these tumors. We are currently characterizing the contribution of CD4+ cells in the progression of these tumor models.
Citation Format: Matthew G. Annis, April A.N. Rose, Ryuhjin Ahn, Brian E. Hsu, Josie Ursini-Siegel, Peter M. Siegel. GPNMB expression modulates the tumor immune microenvironment in mouse models of breast cancer [abstract]. In: Proceedings of the AACR Special Conference on Tumor Immunology and Immunotherapy; 2019 Nov 17-20; Boston, MA. Philadelphia (PA): AACR; Cancer Immunol Res 2020;8(3 Suppl):Abstract nr B88.
A new record of the hoverfly genus Dasysyrphus Enderlein, 1938 (Insecta: Diptera: Syrphidae) from India
Authors: Sengupta Jayita , Naskar Atanu , Maity Aniruddha , Parui Panchanan , Homchaudhuri Sumit , Banerjee Dhriti 
Published: March 2020

One species of genus Dasysyrphus, Enderlein 1938, namely Dasysyrphus albostriatus (Fallen, 1817) is recorded for the first time from India.
First record of Banded Lineblue Prosotas aluta Druce, 1873 (Insecta: Lepidoptera: Lycaenidae) from Bangladesh
Authors: Dey Rajib , Al Haidar Ibrahim Khalil , Rudra Sajib , Islam M. Rafiqul 
Published: March 2020

An individual of Banded Lineblue Prosotas aluta was discovered in Bangladesh on 7 September 2018 from the Kaptai National Park, a mixed evergreen protected forest in the southeastern region of the country. The discovered species was found in the hill stream named ‘Boro Chara’. The photographic documentation confirms the certainty of the species. The occupancy of P. aluta in Asia and distribution around Bangladesh in Cachar and Khasi Hills of India indicate its existence but confirmation was wanting till date. Hence, we report the first confirmed record from Bangladesh.
Hazards of wind turbines on avifauna - a preliminary appraisal within the Indian context
Authors: Deb Himika , Sanyal Tanmay , Kaviraj Anilava , Saha Subrata 
Published: March 2020

Wind farms are substantial sources of renewable energy in India; however, their spread across the country potentially present new hazards to local and migratory birds. This study explored the risk of electrocution and collision of birds with wind turbines close to eco-sensitive zones in India, including Bakkhali, a UNESCO World Heritage site. Geographic information system and remote sensing technology were used. The results indicate vulnerability of local bird species such as barn owl, Indian Scops Owl, Blue Rock Pigeon, Asian Koel, House Crow, Common Sandpiper, Common Snipe, Ruddy Shelduck, Lesser Whistling Duck, Cattle Egret, Great Egret, and Pond Herons, as well as migratory species such as Bar-headed Goose, Red-crested Pochard, and American Black Duck. Modification of wind turbine design and location were considered determinant factors to reduce risk of bird collisions.
Additional morphological notes on the male of Icius alboterminus (Caleb, 2014) (Aranei: Salticidae) with new distribution records from India
Authors: Prajapati Dhruv A. , Kamboj R.D. 
Published: March 2020

This paper includes additional detailed characters and new distribution records of the male of Icius alboterminus (Caleb, 2014) from India. A detailed morphological description based on scanning electron microscopic (SEM) images and illustrations of the reproductive organ are provided.
Do wildlife crimes against less charismatic species go unnoticed? A case study of Golden Jackal Canis aureus Linnaeus, 1758 poaching and trade in India
Authors: Chawla Malaika Mathew , Srivathsa Arjun , Singh Priya , Majgaonkar Iravatee , Sharma Sushma , Punjabi Girish , Banerjee Aditya 
Published: March 2020

Wildlife crimes pertaining to lesser-known species do not usually garner adequate focus or interest by enforcement and conservation agencies. Additionally, illegal wildlife trade fuelled by religious beliefs in sorcery and superstition is an oft-neglected field of research. To draw attention to these two broad issues, we provide a baseline analysis of open-source reports on Golden Jackal Canis aureus poaching and trade in India. We highlight the pervasiveness of an active local and transnational ‘jackal horn’ trade, which is severely under-reported and insufficiently researched. News reports and government seizure data reveal that, between 2013 and 2019, 126 skins, eight tails, more than 370 ‘jackal horns’, 16 skulls and two live jackals have been seized. The demand for the illusionary ‘jackal horn’ appears to be driven by extensive online endorsement and unsubstantiated claims made by religious practitioners, targeted primarily at south Asian markets. This preliminary study is an urgent call for concerted efforts to monitor the illegal trafficking and trade of this common species, with a particular focus on the demand and supply chains.
Thirty-Day Readmissions After Carotid Artery Stenting Versus Endarterectomy
Authors: Lima Fabio V. , Kolte Dhaval , Kennedy Kevin F. , Wang Lily J. , Abbott J. Dawn , Soukas Peter A. , Aronow Herbert D. 
Published: March 2020

Background:
Contemporary, nationally representative 30-day readmissions data after carotid artery stenting (CAS) and carotid endarterectomy (CEA) are lacking.

Methods:
Patients undergoing CAS or CEA were identified from the 2013 to 2014 Nationwide Readmissions Databases. Propensity matching was used to balance baseline clinical characteristics. Thirty-day nonelective readmission rates, length of stay, and causes of readmission were compared.

Results:

Overall, 85 337 (national estimate of 194 332) patients were identified before propensity score matching, 11 490 (13.4%) of whom underwent CAS and 73 847 (86.6%) of whom underwent CEA. Crude 30-day readmission rates were higher for patients treated with CAS than CEA (8.3% versus 6.8%;
P
<0.001), but these differences were negated in the propensity-matched cohort (n=22 214; 8.4% versus 7.9%,
P
=0.20), and readmission length of stay was longer for CEA than CAS (2 versus 1 day, respectively;
P
=0.002). The most common reasons for readmission were neurological and cardiac events; readmission reasons varied by revascularization modality. Readmission due to a stroke or transient ischemic attack was more common among patients treated with CAS than CEA (1.2% versus 0.9%;
P
=0.042), while readmission for procedural or medical complications occurred more often following CEA than CAS (1.1% versus 0.5%;
P
<0.001); readmission rates for cardiac causes were similar between groups.

Conclusions:
Less than 8% of patients are readmitted within 30 days of a carotid revascularization procedure. After adjusting for baseline differences, readmission rates are similar for CAS and CEA although readmission length of stay is longer after the latter. Readmission for neurological causes was more common following CAS while readmission for procedural or medical complications occurred more often following CEA. Higher annual institutional CEA volumes were associated with lower risk for 30-day readmission; in contrast, institutional CAS volumes were not related to readmission risk. These data provide important insights into the short-term, outcomes of patients following carotid artery revascularization.
Characteristics of CXCL2 expression in coronary atherosclerosis and negative regulation by microRNA-421
Authors: Yang Jun , Liu Hu , Cao Qian , Zhong Wei 
Published: March 2020

Objective The study investigated expression of microRNA (miR)-421 in plaques, peripheral blood mononuclear cells (PBMCs), and serum from patients with coronary atherosclerosis. Methods Thirty-three patients with coronary atherosclerosis and 29 healthy individuals were included. Plaque tissue and adjacent intimal tissue were collected from patients. Peripheral blood was collected from patients and healthy individuals. Quantitative real-time PCR was used to determine expression of C-X-C motif chemokine ligand 2 ( CXCL2) mRNA and miR-421. Western blotting was used to measure expression of CXCL2 protein in plaques and PBMCs, and ELISA was used to detect serum levels of CXCL2. A dual luciferase reporter assay was carried out to test whether CXCL2 mRNA directly interacts with miR-421. Results Patients with coronary atherosclerosis had elevated expression of CXCL2 mRNA and protein in plaques, PBMCs, and serum compared with healthy controls but reduced expression of miR-421. The dual luciferase reporter assay showed that miR-421 could bind with the 3′-untranslated seed region of CXCL2 mRNA to regulate its expression. Conclusion We demonstrated that elevated expression of CXCL2 in plaques, PBMCs, and serum of patients with coronary atherosclerosis was related to downregulation of miR-421 expression. miR-421 plays a role in the occurrence of coronary atherosclerosis, probably through CXCL2.
How Do Nursing Organizations Measure Up on Harm Reduction? An Environmental Scan
Authors: Gagnon Marilou , Hazlehurst Emily 
Published: March 2020

Background In the past five years, we have seen a rapid expansion of harm reduction approaches, programs, and policies in Canada. To keep up with the changing policy landscape, a number of Canadian researchers have undertaken projects that seek to analyze policy documents published by provincial and territorial governments. Building on this important body of work, we undertook a similar analysis using documents published by nursing organizations. Purpose To present key findings and propose ways that nursing organizations can strengthen their position on harm reduction. Methods We conducted an environmental scan with a two-part analysis. To complete the first part, we used the 17 quality indicators. To complete the second part, we analyzed the documents for specific harm reduction interventions. Results A total of 39 documents were collected across 76 nursing organizations. The majority of the documents were press or public statements (n = 22), and the most frequently mentioned intervention was supervised injection services (n = 31). On average, documents met 5.6 quality indicators. Documents scored highest on indicator 12 (discuss low-threshold approaches to service provision) and lowest on indicator 3 (acknowledge that not all substance use is problematic). Conclusions Six areas were identified to strengthen nursing organizations’ position on harm reduction.
Patients’ Perspectives and Advice on How to Discuss Sexual Orientation, Gender Identity, and Sexual Health in Oncology Clinics
Authors: Cathcart-Rake Elizabeth , O’Connor Jennifer , Ridgeway Jennifer L. , Breitkopf Carmen Radecki , Kaur Judith S. , Mitchell Jessica , Leventakos Konstantinos , Jatoi Aminah 
Published: March 2020

Objective: This study sought to understand the patients’ perspective of what contributes to an absence of discussions of sexual orientation (SO), gender identity (GI), and sexual health in cancer care. Methods: Patients were recruited from oncology, gynecology, and a gender transition clinic to participate in semistructured interviews, which were analyzed with qualitative methods. Results: A total of 25 patients were interviewed, shedding light on 2 themes. The first was that these conversations are important but infrequent. One patient explained, “…. we know people who have had sex changes…[they] would have appreciated that question.” In response to whether sexual health was ever brought up, one patient responded, “No doctor ever has.” Patients described unaddressed issues: “There have been times, you know, we’ve wondered if it was okay to make love.” The second theme consisted of 4 pragmatic, patient-provided points to facilitate discussions: (1) implementation of a scale of 1 to 10 (with 10 being comfortable) to first gauge patients’ comfort in talking about SO, GI, and sexual health; (2) having the health-care provider explore the topic again over-time; (3) making sure the health-care provider is comfortable, as such comfort appears to enhance the patient’s comfort (“I have a doctor here, a female doctor, who just matter of fact will ask if I get erections and so on because of the medication she’s giving me);” and (4) eliminating euphemisms (one patient stated, “I don’t know what you mean by ‘sexual health’.”). Conclusion: Oncology health-care providers have a unique opportunity and responsibility to address SO, GI, and sexual health.
Using Therapeutic Beat Making and lyrics for empowerment
Authors: Travis Raphael , Gann Elliot , Crooke Alexander HD , Jenkins Susan M 
Published: March 2020

Summary Mental health remains a significant issue among young people in the US and elsewhere. Evidence of the beneficial role Hip Hop-based approaches can play in this space is growing. The value of such approaches centers on links to community, culture, and the therapeutic potential of practicing certain Hip Hop elements. Yet, while there has been a steady growth in Hip Hop-based approaches, little work has explored how they can overlap, complement, or reinforce each other. In response, this article explores the theoretical synergies between two established Hip Hop-based models – “Hip Hop and Empowerment” (HHE) and “Therapeutic Beat Making” (TBM) – to offer an integrated approach that draws on the complementarity between and uniqueness of each. Findings Authors identify an integrated HHE–TBM model for supporting youth mental health which forefronts sense of community, cultural relevance, social justice, and empowerment. Authors outline a practice model, which leverages the best of activities, reflections, and discussions, and indicate its potential in working adaptively with diverse communities of young people. Applications When engaging Hip Hop for therapeutic means, whether for health promotion or complex therapy, it is a dynamic, creative, culturally sensitive, regulating, and developmental means for finding common ground and creating rapport. It bridges creative and insight-oriented practices. It embraces the ability to generate emotional responses from creating and analyzing music, including esthetic emotions (i.e. appreciation (of qualities)), utilitarian emotions (i.e. functional, motivational, relevant to immediate well-being, goal-oriented), and epistemic emotions (i.e. gained knowledge or insight, awareness).
The natural history of progressive fibrosing interstitial lung diseases
Authors: Brown Kevin K. , Martinez Fernando J. , Walsh Simon L.F. , Thannickal Victor J. , Prasse Antje , Schlenker-Herceg Rozsa , Goeldner Rainer-Georg , Clerisme-Beaty Emmanuelle , Tetzlaff Kay , Cottin Vincent , Wells Athol U. 
Published: March 2020

We used data from the INBUILD and INPULSIS trials to investigate the natural history of progressive fibrosing interstitial lung diseases (ILDs).Subjects in the two INPULSIS trials had a clinical diagnosis of idiopathic pulmonary fibrosis (IPF) while subjects in the INBUILD trial had a progressive fibrosing ILD other than IPF and met protocol-defined criteria for ILD progression despite management. Using data from the placebo groups, we compared the rate of decline in forced vital capacity (FVC) (mL·year−1) and mortality over 52 weeks in the INBUILD trial with pooled data from the INPULSIS trials.The adjusted mean annual rate of decline in FVC in the INBUILD trial (n=331) was similar to that observed in the INPULSIS trials (n=423) (−192.9 mL·year−1and −221.0 mL·year−1, respectively; nominal p-value=0.19). The proportion of subjects who had a relative decline in FVC >10% predicted at Week 52 was 48.9% in the INBUILD trial and 48.7% in the INPULSIS trials, and the proportion who died over 52 weeks was 5.1% in the INBUILD trial and 7.8% in the INPULSIS trials. A relative decline in FVC >10% predicted was associated with an increased risk of death in the INBUILD trial (hazard ratio 3.64) and the INPULSIS trials (hazard ratio 3.95).These findings indicate that patients with fibrosing ILDs other than IPF, who are progressing despite management, have a subsequent clinical course similar to patients with untreated IPF, with a high risk of further ILD progression and early mortality.
Can the science of Prosocial be a part of evolution education?
Authors: Eirdosh Dustin , Hanisch Susan 
Published: March 2020

AbstractWe provide a brief overview of Prosocial: Using Evolutionary Science to Build Productive, Equitable, and Collaborative Groups by Paul Atkins, David Sloan Wilson, and Steven Hayes. The book offers a range of promising content for evolution education, and yet also highlights core conceptual challenges in modern evolution science discourse that educators and researchers aiming to improve evolution education may find beneficial to strategically engage with as a scientific community. We discuss these challenges and opportunities with a view towards implications for evolution education research and practice.