bluuebunny/crossref_metadata_2025_split huggingface dataset:
A High-Efficiency Energy Harvesting By Using Hydraulic Electromagnetic Regenerative Shock Absorber
Authors: Iqbal Muhammad Yousaf , Wu Zhifei , Mahmood Khalid 
Published: June 2020

Abstract
This article intends a hybrid energy harvesting shock absorber design which comprehends energy harvesting of automobile suspension vibration dissipation. A mathematical model of the energy harvesting prototype is established, and simulation results show that the dissipation energy can be recovered by varying the feed module, thereby got the damping forces ratio at different compression and extension stroke. The energy conversion from hydraulic energy to mechanical energy mainly then mechanical energy converted into electrical energy furthermore we can rechange our battery from this recovered energy. The advanced mathematical model and prototype proposed maximum ride comfort meanwhile recovered the suspension energy and fuel saving. This article shows the simulation results verifying it with prototype test results. The damping force of expansion stroke is higher than the damping force of compression stroke. The damping characteristics curves and speed characteristics curves verify the validity by simulation and prototyping damper at different amplitudes of off-road vehicles. The Hydraulic Electromagnetic Regenerative Shock Absorber (HESA) prototype characteristic is tested in which 65 watts recovered energy at 1.67 Hz excitation frequency. So, 14.65% maximum energy recovery efficiency got at 20 mm rod diameter and 8 cc/rev motor displacement. The damping characteristics of the HESA prototype examined and it has ideal performance as the standard requirements of the National Standard QC/T 491–1999.
Accuracy of routine biomarkers and blood leucocytes count to assist diagnosis of COVID-19-associated pneumonia in adult patients visiting the emergency department
Authors: Maincent Cécile , Berthier Frédéric , Farhad Ryan Lukas , Fissore-Magdelein Cristel , Dittlot Claire Claire , Beau Nathalie , Lépine Jérémy , Lorillou Marjorie , Dietz Matthieu , Liberatore Mathieu , Pathak Atul , Faraggi Marc Marc , Chaillou-Orpitz Sylvie , Keita-Perse Olivia , Chironi Gilles , Perrin Christophe , Claessens Yann-Erick 
Published: June 2020

Abstract
Background To investigate whether routine biomarkers and blood leucocytes count could assist diagnosis of COVID-19-associated pneumonia in adult patients visiting the emergency department (ED). Methods This monocentre retrospective study enrolled 254 patients with nasopharyngeal RT-PCR for SARS-COV-2, routine biomarkers (D-dimers, fibrinogen, C-reactive protein, procalcitonin, NTpro-BNP, cTnT-hs) and blood cell counts. Sensitivity and specificity were evaluated. An adjudication committee classified diagnostic probability as certain, probable, unlikely, and excluded, based on all available data, then distributed in 2 categories: high (certain and probable) and low probability (unlikely and excluded). Results Between 25 th of February and 15 th of April, 2020, 254 of 388 patients could be analyzed. The adjudication committee classified 46 patients as definite, 18 as probable, 64 as unlikely, and 126 as excluded, corresponding to 64 high and 190 low probability. High and low probability patients differed for fibrinogen (P<0.0005) and white blood cell counts, notably leucocytes (P=0.0015), neutrophilic (P=0.0036), lymphocytes (P=0.0057), eosinophilic (P=0.027), and basophilic (P<0.001) counts. In a multivariate analysis, basophilic count < 25/µL (OR 3.048 [95%CI; 1.34-6.919]), neutrophilic count < 4000 /µL (OR 5.525 [95%CI; 2.20-13.855], and fibrinogen > 3g/L (OR 6355 [95%CI; 2.01-20.079] were independently associated with the diagnosis. Negative predictive values were 0.98 and 0.93 combining fibrinogen ( < 3g/L) and eosinophilic count ( < 80/µL), and fibrinogen and basophilic count ( < 25/µL), respectively. Conclusion Changes in fibrinogen and white blood cells, notably basophilic count, showed interesting performance for the diagnosis COVID-19 associated pneumonia. Combining fibrinogen with either eosinophilic or basophilic count was helpful to exclude the diagnosis.
Understanding HIV and Associated Risk Factors Among Religious Groups in Zimbabwe
Authors: Mapingure Munyaradzi Paul , Mukandavire Zindoga , Chingombe Innocent , Cuadros Diego , Mutenherwa Farirai , Mugurungi Owen MacDonald , Musuka Godfrey Nyangadzai 
Published: June 2020

Abstract
BackgroundThe influence of religion and belief systems is widely recognized as an important factor in understanding of health risk perception and myths in the general fight against the HIV pandemic. This study compares the understanding of HIV risk factors and utilization of some HIV services among religious groups in Zimbabwe Methods: We conducted statistical analysis to investigate the understanding of HIV and associated risk factors among religious groups in Zimbabwe using Zimbabwe Demographic and Health Survey (ZDHS) data.MethodsWe conducted statistical analysis to investigate the understanding of HIV and associated risk factors among religious groups in Zimbabwe using 20015-2016 Zimbabwe Demographic and Health Survey (ZDHS) data. We began by investigating on associations explaining of HIV risk factors among religious groups. A ranking procedure was conducted to compare the understanding of HIV risk for different religions. A multivariate stepwise backward elimination method was carried out to explore on factors determining understanding of HIV risk after controlling for confounding factors using the most recent ZDHS data (2015-2016). Analysis of the 2010-11, 2005-6 rounds of ZDHS data was also conductedResultsThe results from the three surveys showed that, in general apostolic sector had low understanding of HIV risk factors compared to other religious groups. Analysis of the 2015-2016 ZDHS data showed that women belonging to the apostolic sector were less likely to know where to get an HIV test odds ratio (OR) and 95% confidence interval, 0.665 (0.503-0.880) and to know that male circumcision reduces HIV transmission OR 0.863 (0.781-0.955). Women from this group did not know that circumcised men can be infected if they do not use condoms OR 0.633 (0.579-0.693), nor that it is possible for a healthy-looking person to have HIV, OR 0.814 (0.719-0.921). They would not buy vegetables from a vendor with HIV OR 0.817 (0.729-0.915) and were less likely to support that HIV positive children should be allowed to attend school with HIV negative children OR 0.804 (0.680-0.950). Similar results were obtained for men in the apostolic sector. These men also did not agree that women were justified to use condoms if the husband has an STI OR 0.851 (0.748-0.967).Conclusions: Our results suggest that apostolic sector lack adequate knowledge of HIV and associated risk factors than other religious groups. Targeting HIV prevention programmes by religious groups could be an efficient approach for controlling HIV in Zimbabwe.
LncRNA HOXA-AS2 Facilitates Cervical Cancer Progression and Angiogenesis via miR-509-3p/BTN3A1 Axis
Authors: Chen Ruxiang , He Ping 
Published: June 2020

Abstract
Background: Cervical cancer, the leading cause of cancer-relevant mortality in females, is an aggressive malignant tumor. Tumor angiogenesis is vital for cell proliferation and metastasis in cancers. Accumulating studies have claimed that long non-coding RNAs (lncRNAs) participate in the progression of various cancers. The aim of this research is to explore the biological role and regulatory mechanism of LncRNA HOXA-AS2 in cervical cancer.Methods: Experiments including RT-qPCR, western blot, RIP, MTT, EdU, transwell, luciferase reporter, RIP, FISH, tube formation assays were applied to investigate the biological role and regulatory mechanism of LncRNA HOXA-AS2 in cervical cancer.Results: In current study, the results disclosed that HOXA-AS2 was notably upregulated in cervical cancer tissues and cell lines, and high HOXA-AS2 expression was strongly associated with poor prognosis of cervical cancer patients. Furthermore, HOXA-AS2 contributed to cell proliferation, migration, invasion and angiogenesis in cervical cancer. In addition, HOXA-AS2 absorbed miR-509-3p and miR-509-3p targeted BTN3A1 in cervical cancer. Besides, BTN3A1 overexpression partly rescued the inhibitory influence of HOXA-AS2 knockdown on cervical cancer progression and angiogenesis. Overall, HOXA-AS2 promoted cervical cancer progression and angiogenesis through sponging miR-509-3p to elevate BTN3A1 expression. Conclusions: In other words, this paper was the first to study the molecular regulatory mechanism of HOXA-AS2 in cervical cancer and certified that HOXA-AS2 accelerated progression and angiogenesis in cervical cancer by targeting miR-509-3p/BTN3A1 axis, which may become a beneficial therapeutic target for cervical cancer.
Impact of the COVID-19 pandemic on willingness to adopt healthy dietary habits in China
Authors: Xu Ying , Li Zhi-xue , Yu Wei-jun , Liu Zheng , He Xiang-yang , Ma Yan , Cai Feng-min , Wang De-wang , Guo Yan-Fang , Chen Jialong 
Published: June 2020

Abstract
Background: To investigate impact of the 2019 novel coronavirus disease (COVID-19) pandemic on willingness to adopt healthy dietary habits in China.Methods: A survey was carried out, and subjective perception of impact due to COVID-19 and willingness to change dietary habits were obtained.Results: A total of 22,459 subjects were derived from China, with an average age of 27.9±7.8 years old. Of them, the mean score of willingness to adopt healthy dietary habits was 2.2 (ranges from -9 to 9). Multivariate regression analysis showed that the impact of the COVID-19 pandemic (epidemic concern, impact of psychology, impact of work or study) are associated with a higher score of willingness to adopt healthy dietary habits among female, the older, on-medical worker, and individuals married or with higher education level, normal BMI.Conclusions: There was a positive improvement to a proper diet, so the changing features of diets should be considered in nutritional interventions for maintaining health, and prevention and control COVID-19 during the pandemic period.
Obtaining and Main Dielectric Properties of Ba0.6Pb0.4TiO3/graphene Oxide Composite
Authors: Skulski Ryszard , Bochenek Dariusz , Brzezińska Dagmara , Stobinski Leszek , Niemiec Przemysław , Dercz Grzegorz , Osińska Katarzyna 
Published: June 2020

Abstract
In this paper there is described the technology of obtaining and results of investigations of microstructure, XRD, SEM, main dielectric properties, electrical conductivity measurements and P-E hysteresis loops of Ba0.6Pb0.4TiO3/graphene oxide composite (abbr. BPT/GO). In the final step of technology, the samples have been sintered using the Spark Plasma Sintering (SPS) method. Diffraction patterns of BPT/GO composite exhibit lines which can be related with perovskite structure, as well as reveal additional lines that can be derived from the initial component oxides. Investigations of electrical conductivity suggest that the PTCR effect occurs at temperatures up to about 120°C. Dielectric hysteresis loops below 90°C are wide and typical for materials with rather high electrical conductivity. The hysteresis loop obtained at 120°C is more typical for ferroelectrics. The obtained material is interesting, however it is probably possible to find better conditions of obtaining it and/or a better composition.
A robust eleven-genes prognostic model can predict overall survival in bladder cancer patients based on five cohorts
Authors: Lin Jiaxing , Yang Jieping , Xu Xiao , Wang Yutao , Yu Meng , Zhu Yuyan 
Published: June 2020

Abstract
Background: Bladder cancer is the tenth most common cancer in the world, but existing biomarkers and prognostic models are limited.Method: In this study, we used four bladder cancer cohorts from The Cancer Genome Atlas and Gene Expression Omnibus databases to perform univariate Cox regression analysis to identify common prognostic genes. We used selected genes to construct a prognostic model. Kaplan-Meier analysis, Receiver Operating Characteristic curve, and univariate and multivariate Cox analysis were used to evaluate the prognostic model for the four cohorts. Finally, a co-expression network, CIBERSORT, and ESTIMATE algorithm were used to explore the mechanism related to the model.Results: A total of 11 genes were identified from the four cohorts to construct the prognostic model, including eight risk genes (SERPINE2, PRR11, DSEL, DNM1, COMP, ELOVL4, RTKN, and MAPK12) and three protective genes (FABP6, C16orf74, and TNK1). The model and the 11 genes have excellent performance in predicting overall survival and have been confirmed in 5 cohorts. The model's predictive ability is stronger than other clinical features and has practical significance in clinical application.Through the analysis of the weighted co-expression network, the gene module related to the model was found, and the key genes in this module were mainly enriched in the items related to the tumor microenvironment. When comparing the level of immune cell infiltration in high-risk samples, B cell memory showed low infiltration in high-risk patients. Furthermore, in the case of low B cell memory infiltration and high-risk score, the prognosis of the patients was the worst.Conclusion: The model we developed has strong stability and good performance and can stratify the risk of bladder cancer patients, to achieve individualized treatment.
Haematopoietic Score for the Prognostic Evaluation of Multiple Myeloma Patients Undergoing First-line Treatment With a Bortezomib-based Regimen
Authors: He JingSong , Yue XiaoYan , Han XiaoYan , He DongHua , Zhao Yi , Yang Yang , Zheng GaoFeng , Wu WenJun , Cai Zhen 
Published: June 2020

Abstract
Backgroud: It is very important to evaluate the prognosis of multiple myeloma (MM) patients before starting treatment. Although haematopoietic status may have a significant impact on patient survival, it has not received sufficient attention in current clinical practice.Methods:This was a retrospective study of 150 newly diagnosed MM patients treated in one hematonosis treatment centre from May 2013 to June 2016, all of whom received first-line treatment with a bortezomib-based regimen.The effects of haematopoietic factors, including haemoglobin levels (Hgb <100 g/L), mean corpuscular volume (MCV>99.1 fL), and platelet count (<150 × 10E9/L), on the prognosis of the patients were analysed. Each of the above factors was assigned a value of 1 to generate a haematopoietic score.Results: According to the results, 59 (39.3%) patients had a score of 0, 43 (28.7%) had a score of 1, 29 (19.3%) had a score of 2, and 19 (12.7%) had a score of 3. The median PFS times were 43.1, 24.5, 32.6 and 14.2 months, respectively (P<0.001), and the median OS times were NR, 47.1 months, NR and 31.4 months, respectively (P<0.001). Multivariable analysis showed that the R-ISS stage (3 vs 1-2, HR, 1.4), haematopoietic score (3 vs 0-2, HR, 1.91) and plasma cell percentage (>30%, HR, 1.96) are independent prognostic predictors for PFS; age (≤65 years, HR, 0.54), the Durie-Salmon stage (3B vs 1-3a, HR, 2.96), haematopoietic score (3 vs 0-2, HR, 2.53) and the bone marrow plasma cell percentage (>30%, HR, 2.35) are independent prognostic predictors of OS. Conclusion: This study suggests that the haematopoietic score can be used to evaluate the prognosis of newly treated MM patients in the era of new drugs. However, there is still a need to enlarge the number of cases and carry out prospective research to validate this conclusion.
The Age Threshold of the 8th Edition AJCC Classification Is Useful for Indicating Patients with Aggressive Papillary Thyroid Cancer in Clinical Practice .
Authors: Kaliszewski Krzysztof , Diakowska Dorota , Nowak Łukasz , Wojtczak Beata , Rudnicki Jerzy 
Published: June 2020

Abstract
Background: Papillary thyroid cancer (PTC) is unique among cancers in that patient age is a consideration in staging. One of the most important modifications in the 8th Edition of the American Joint Committee on Cancer (AJCC) classification, which was introduced in 2018, was to increase the age cutoff for risk stratification in PTC from 45 to 55 years. However, whether this cutoff is useful in clinical practice remains controversial. In the present study, we assessed how well this new age threshold stratifies patients with aggressive PTC.Methods: We retrospectively analyzed the clinicopathological features and overall survival rate of 523 patients with PTC admitted to and surgically treated at a single surgical center. We divided the patients into two groups according to age at PTC diagnosis: ≥55 years and <55 years. Results: We found that the rates of tumor progression, lymph node metastasis (LNM) and distant metastasis were significantly higher in patients ≥55 years than in those <55 years; consequently, TNM stages were significantly higher in older than in younger patients (p<0.05 for all parameters). The risk of tumor progression (T3+T4) was nearly two-fold higher and the risk of LNM (N1) more than four-fold higher in older than in younger patients (p<0.05 for both). No patients <55 years old but 19 patients >55 years old (9.8% of the total group) showed distant metastasis. The rates of microcalcification, vascular and capsular invasion, extrathyroidal extension, irregular tumor shape, multifocality, bilaterality and multiplicity of foci were significantly higher in older than in younger patients (p<0.05 for all). The rate of disease-free survival was significantly lower in older (86.6%) than in younger (98.7%) patients (p<0.0001), and the rate of overall survival was significantly lower in older (90.3%) than in younger (99.4%) patients (p<0.0001).Conclusions: PTC is more aggressive in patients aged ≥ 55 years than in their younger counterparts. This age therefore effectively stratifies PTC patients with a poor prognosis, indicating it is likely to be useful in clinical practice.
Endosomal Dysfunction in iPSC-Derived Neural Cells from Parkinson’s Disease Patients with VPS35 D620N
Authors: Bono Keiko , Miyauchi-Hara Chikako , Sumi Shunsuke , Oka Hisayoshi , Iguchi Yasuyuki , Okano Hirotaka James 
Published: June 2020

Abstract
Mutations in the Vacuolar protein sorting 35 ( VPS35 ) gene have been linked to familial Parkinson’s disease (PD), PARK17. VPS35 is a key component of the retromer complex, which plays a central role in endosomal trafficking. However, whether and how VPS35 deficiency or mutation contributes to PD pathogenesis remain unclear. Here, we analyzed human induced pluripotent stem cell (iPSC)-derived neurons from PD patients with theVPS35D620N mutation and addressed relevant disease mechanisms. In the disease group, dopaminergic (DA) neurons underwent extensive apoptotic cell death. Furthermore, the movement of Rab5a - or Rab7a - positive endosomes was slower , and the endosome fission and fusion frequencies were lower in the PD group than in the healthy control group . Interestingly , vesicles positive for cation-independent mannose 6-phosphate receptor transported by retromers were abnormally localized in glia-like cells derived from patient iPSCs. These results suggest that VPS35 mutation causes endosomal dysfunction in neural cells in PARK17.
Molecular analysis of targeted insecticide resistance gene mutations in field Aedes aegypti and Culex quinquefasciatus mosquito vectors of arboviruses from Saudi Arabia
Authors: Fang Yuan , Ernest Tambo , Xue Jing-Bo , Zhang Yi , Kamal Hany A. , Alzahrani Fahd S. , Alzahrani Mohamed H. , Zhou Xiao-Nong , Khater Emad I.M. 
Published: June 2020

Abstract
Background: Gene mutations on target sites can be a valuable reference to the status of insecticide resistance. Jeddah, a global commercial and major port-of-entry city, is bearing the most (>70%) dengue disease burden and the population of the dengue vector Aedes aegypti in Saudi Arabia. Culex quinquefasciatus is a second example as one of the major arboviral vectors in the region. However, the status of insecticide resistance and targeted site mutations on the responsible genes are not fully characterized.Methods: We randomly sampled both mosquito species, Ae. aegypti and Cx. quinquefasciatus across Jeddah by daily mosquito surveillance in 2016 to detect the resistance-associated target site mutations on the voltage gated sodium channel (VGSC) and acetylcholinesterase 1 (ace-1) genes. Results: Our findings showed that Ae. aegypti resistance-associated VGSC gene mutations revealed polymorphic mutations on the 989, 1016, and 1534 sites. Additionally, we documented two types of introns between exons 20 and 21, however, the I1011M point mutation was undetected. Linkage disequilibrium associations were shown between V1016G with S989P, V1016G with F1534, and V1016G with type A intron. Furthermore, no mutation on ace-1 was identified in Ae. aegypti. In Cx. quinquefasciatus, homozygous L1014F/L1014F (95.23%) on the VGSC and heterozygous G119/G119S (100%) on ace-1 were widely distributed in the samples studied. Analysis of intron sequences obtained in our study and homologous sequences retrieved from GenBank showed that the intron type was significantly associated with 1016 (P = 0.000) allele type, and may reflect the history of insecticide treatment in different continents.Conclusions: To the best of our knowledge, this is the first record of the intron types between exons 20 and 21 on VGSC of Ae. aegypti populations, and the first report of insecticide resistance gene mutation being present in field caught Cx. quinquefasciatus in Jeddah, Saudi Arabia. High prevalence of insecticide resistance gene mutations in local primary mosquito vector species alert the urgent need to carry out a comprehensive insecticide resistance gene mutation surveillance and monitoring to guide sustained and effective integrated vector management planning and innovative guidelines in local predominate mosquito populations and mosquito-borne disease control and elimination in Saudi Arabia.
Heterologous Expression and Application of Multicopper Oxidases from Enterococcus spp. for Degradation of Biogenic Amines
Authors: Li Binbin , Wang Yuan , Xue Linlin , Lu Shiling 
Published: June 2020

Background:
Biogenic amines are harmful to human health at a certain extent. As a
kind of biogenic amine oxidase, multicopper oxidase can be used to degrade them. Currently, the
literature about enzyme from Enterococcus spp. are limited, and recombinant multicopper oxidase
might be an effective way to degrade biogenic amines.

Objective:
(i) Select and identify strains that can degrade biogenic amines, (ii) overexpress enzyme
from Enterococcus spp., (iii) measure gene expression and probe amine-degradation differences
among strains (native, E. coli DH5α, and L. delbruckii), and (iv) examine the biochemical properties
of recombinant multicopper oxidase, (v) apply the recombinant enzyme into smoked horsemeat
sausage.

Methods:
Reverse transcription PCR and high-performance liquid chromatography were performed
to examine gene expression and amine degradation rate.

Results:
The results demonstrated that target enzymes were successfully overexpressed, accompanied
by increased amine-degrading activity (P <0.05). Gene from E. faecalis M5B was expressed
in L. delbrueckii resulted in degradation rates for phenylethylamine, putrescine, histamine
and tyramine of 54%, 52%, 70% and 40%, respectively, significantly higher than achieved by other
recombinant strains.

Conclusion:
In this work, gene expression levels were higher in recombinant M5B than recombinant
M2B, regardless of host. E. coli is more stable to express multicopper oxidase. Besides, the
amine-degrading ability was markedly increased in the two recombinant strains. After prolonged incubation,
the recombinant enzyme could degrade three amines, and it displayed high alkali resistance
and thermostability.