bluuebunny/crossref_metadata_2025_split huggingface dataset:
Phyto-Fabrication of Different Nanoparticles and Evaluation of their Antibacterial and Anti-Biofilm Efficacy
Authors: Ingle Avinash P. , Wagh Shweta , Biswas Jayantakumar , Mondal Monojit , Feitosa Chistiane Mendes , Rai Mahendra 
Published: March 2020

Background:
Nowadays, microbial infections are considered the most important causes of
morbidity and mortality. Various microorganisms including biofilm-forming bacteria reported playing
a crucial role in such public health concerns. On the other hand, the inefficacy of conventional
antimicrobial agents due to various reasons including drug resistance creates a need for the development
of effective drug molecules. In this context, nanoparticles can be promisingly used in the management
of all such concerns.

Objective:
The study aimed to evaluate antibacterial and biofilm removal efficacy of various metal
nanoparticles against biofilm-forming bacteria.

Methods:
Various metal nanoparticles including silver (AgNPs), copper (CuNPs) and zinc oxide
(ZnONPs) were synthesized from plant leaf extracts of Punica granatum, Citrus medica, and Annona
squamosa, respectively.

Results:
Thus synthesized nanoparticles were evaluated for their antibacterial and biofilm removal
efficacies against Enterococcus gallinarum, Staphylococcus haemolyticus, Enterobacter aerogenes,
and Salmonella enterica serovar Typhi to propose a promising approach towards management of
such pathogens. In vitro antibacterial study demonstrated that AgNPs showed the maximum efficacy
against all the test bacteria followed by CuNPs and ZnONPs.

Conclusion:
Our results indicate that the metal nanoparticles can be effectively used for the removal
of biofilm and also as antimicrobial agents for the management of microbial pathogens responsible
for dreadful infections.
Antibiofilm Action of ZnO, SnO2 and CeO2 Nanoparticles Towards Grampositive Biofilm Forming Pathogenic Bacteria
Authors: Khan Fazlurrahman , Lee Jang-Won , Pham Dung N.T. , Khan Mohammad M. , Park Seul-Ki , Shin Il-Shik , Kim Young-Mog 
Published: March 2020

Background:
The ability to form biofilm and produce several virulence factors has caused
numerous human pathogens to become tremendously resistant towards traditional antibiotic treatments,
thus, new alternative strategies are urgently in demand. One of the strategies that have recently been developed
involves the application of metallic Nanoparticles (NPs). Up to the present, promising results in
terms of antimicrobial and antibiofilm activities have been observed in a wide range of metal NPs.

Methods:
The present study has selected three metal oxides such as ZnO, SnO2 and CeO2 NPs to comparatively
investigate their antibiofilm and antibacterial properties against two Gram-positive human
pathogens, which are Listeria monocytogenes and Staphylococcus aureus.

Result:
The anti-biofilm activities of ZnO, SnO2 and CeO2 NPs against S. aureus and L. monocytogenes
were assayed by crystal violet staining and confirmed by microscopic visualization using SEM.
The synthesis of amyloid protein by S. aureus and exopolysaccharide by L. monocytogenes in the presence
of ZnO, SnO2 and CeO2 NPs was evaluated by Congo red assay.

Conclusion:
Overall, these results indicated that ZnO, SnO2 and CeO2 NPs can be considered as potential
agents for treating the infections caused by L. monocytogenes and S. aureus, especially those associated
with biofilm formation. Based on the present study, further studies are required to understand
their mechanisms at both phenotypic and molecular levels, as well as their in vivo cytotoxicity, thereby
enabling the applications of these metal oxide NPs in biomedical fields and food industry.

Discussion:
Results have shown that ZnO, SnO2 and CeO2 NPs effectively inhibited biofilm formation
of both L. monocytogenes and S. aureus. The microscopic analysis also confirmed the antibiofilm activity
of these NPs. It was also found that only ZnO NPs inhibited cell growth as well as the production
of amyloid protein in S. aureus.
Comparative Usability Analysis and Parental Preferences of Three Web-Based Knowledge Translation Tools: Multimethod Study
Authors: Anzinger Harrison , Elliott Sarah Alexandra , Hartling Lisa 
Published: March 2020

Background
Connecting parents to research evidence is known to improve health decision making. However, guidance on how to develop effective knowledge translation (KT) tools that synthesize child-health evidence into a form understandable by parents is lacking.

Objective
The aim of this study was to conduct a comparative usability analysis of three Web-based KT tools to identify differences in tool effectiveness, identify which format parents prefer, and better understand what factors affect usability for parents.

Methods
We evaluated a Cochrane plain language summary (PLS), Blogshot, and a Wikipedia page on a specific child-health topic (acute otitis media). A mixed method approach was used involving a knowledge test, written usability questionnaire, and a semistructured interview. Differences in knowledge and usability questionnaire scores for each of the KT tools were analyzed using Kruskal-Wallis tests, considering a critical significance value of P=.05. Thematic analysis was used to synthesize and identify common parent preferences among the semistructured interviews. Key elements parents wanted in a KT tool were derived through author consensus using questionnaire data and parent interviews.

Results
In total, 16 parents (9 female) with a mean age of 39.6 (SD 11.9) years completed the study. Parents preferred the Blogshot over the PLS and Wikipedia page (P=.002) and found the Blogshot to be the most aesthetic (P=.001) and easiest to use (P=.001). Knowledge questions and usability survey data also indicated that the Blogshot was the most preferred and effective KT tool at relaying information about the topic. Four key themes were derived from thematic analysis, describing elements parents valued in KT tools. Parents wanted tools that were (1) simple, (2) quick to access and use, and (3) trustworthy, and which (4) informed how to manage the condition. Out of the three KT tools assessed, Blogshots were the most preferred tool by parents and encompassed these four key elements.

Conclusions
It is important that child health evidence be available in formats accessible and understandable by parents to improve decision making, use of health care resources, and health outcomes. Further usability testing of different KT tools should be conducted involving broader populations and other conditions (eg, acute vs chronic) to generate guidelines to improve KT tools for parents.
A Late Attempt to Involve End Users in the Design of Medication-Related Alerts: Survey Study
Authors: Baysari Melissa Therese , Zheng Wu Yi , Van Dort Bethany , Reid-Anderson Hannah , Gronski Mihaela , Kenny Eliza 
Published: March 2020

Background
When users of electronic medical records (EMRs) are presented with large numbers of irrelevant computerized alerts, they experience alert fatigue, begin to ignore alert information, and override alerts without processing or heeding alert recommendations. Anecdotally, doctors at our study site were dissatisfied with the medication-related alerts being generated, both in terms of volume being experienced and clinical relevance.

Objective
This study aimed to involve end users in the redesign of medication-related alerts in a hospital EMR, 4 years post implementation.

Methods
This work was undertaken at a private not-for-profit teaching hospital in Sydney, Australia. Since EMR implementation in 2015, the organization elected to implement all medication-related alert types available in the system for prescribers: allergy and intolerance alerts, therapeutic duplication alerts, pregnancy alerts, and drug-drug interaction alerts. The EMR included no medication administration alerts for nurses. To obtain feedback on current alerts and suggestions for redesign, a Web-based survey was distributed to all doctors and nurses at the site via hospital mailing lists.

Results
Despite a general dissatisfaction with alerts, very few end users completed the survey. In total, only 3.37% (36/1066) of doctors and 14.5% (60/411) of nurses took part. Approximately 90% (30/33) of doctors who responded held the view that too many alerts were triggered in the EMR. Doctors suggested that most alerts be removed and that alerts be more specific and less sensitive. In contrast, 97% (58/60) of the nurse respondents indicated that they would like to receive medication administration alerts in the EMR. Most nurses indicated that they would like to receive all the alert types available at all severity levels.

Conclusions
Attempting to engage with end users several years post implementation was challenging. Involving users so late in the implementation process may lead to clinicians viewing the provision of feedback to be futile. Seeking user feedback on usefulness, volume, and design of alerts is extremely valuable; however, we suggest this is undertaken early, preferably before system implementation.
Effectiveness of a Mobile eHealth App in Guiding Patients in Pain Control and Opiate Use After Total Knee Replacement: Randomized Controlled Trial
Authors: Pronk Yvette , Peters Maud Cornelia Wilhelmina Maria , Sheombar Amarsing , Brinkman Justus-Martijn 
Published: March 2020

Background
Little is known about pain and opiate use at home directly after total knee replacement (TKR). Due to adverse effects, low opiate use is desired. An electronic health app (PainCoach) was developed to guide patients in pain control and opiate use.

Objective
The aim of this paper was to investigate the effects of the PainCoach app on pain control and opiate use in patients who underwent TKR during the first 2 weeks at home after surgery.

Methods
In an unblinded randomized controlled trial, patients scheduled for TKR were offline recruited and randomized to a PainCoach group or control group. In the PainCoach group, the PainCoach app was downloaded on each patient’s smartphone or tablet. In response to the patient’s input of the pain experienced, the PainCoach app gave advice on pain medication use, exercises/rest, and when to call the clinic. This advice was the same as that received during usual care. The control group received usual care. The primary outcomes were opiate use and visual analog scale (VAS) pain scores at rest, during activity, and at night during the first 2 weeks at home after surgery, which were collected daily from day 1 until 14 postoperatively by online questionnaires. The actual amount of app use was recorded, and active use was defined as ≥12 total app uses.

Results
The pain scores did not differ between the groups. The PainCoach group (n=38) used 23.2% less opiates (95% CI −38.3 to −4.4; P=.02) and 14.6% more acetaminophen (95% CI 8.2-21.3; P<.001) when compared with the findings in the control group (n=33). The PainCoach app was used 12 (IQR 4.5-22.0) times per patient. In the active PainCoach subgroup (n=19), the following were noted when compared with the findings in the control group: 4.1 times faster reduction of the VAS pain score during activity (95% CI −7.5 to −0.8; P=.02), 6.3 times faster reduction of the VAS pain score at night (95% CI −10.1 to −2.6; P=.001), 44.3% less opiate use (95% CI −59.4 to −23.5; P<.001), 76.3% less gabapentin use (95% CI −86.0 to −59.8; P<.001), and 21.0% more acetaminophen use (95% CI 12.6-30.0; P<.001).

Conclusions
The use of the PainCoach app contributes to reduced opiate use in the initial period at home after TKR. Active use of this app leads to a further reduction in opiate use and improved pain control.

Trial Registration
ClinicalTrials.gov NCT03961152; https://clinicaltrials.gov/ct2/show/NCT03961152
ENIGMATIC INDUCTION OF CYTOMIXIS IN ALLIUM CEPA ROOT MERISTEM BY AGLAIA EDULIS ROXB. LEAF EXTRACT AND ITS PHYTOCHEMICAL RATIONALE
Authors: RAVINDRAN ARCHANA ELAMKULAM , THOPPIL JOHN ERNEST 
Published: March 2020

Objective: The present study aims to analyze the potential of Aglaia edulis Roxb. leaf extract to induce cytological aberrations in Allium cepa root meristem and to determine the phytoconstituents in the extract.
Methods: Cytotoxicity evaluation of the leaf methanolic extract was done using Allium cepa assay using various concentrations. Volatile phytoconstituents in the extract were determined using gas chromatography–mass spectrometry analysis.
Results: Considerable number of cytomictic cells along with other aberrations was observed. The occurrence of cytomixis was found to be dose dependent where it ranged from 6.58±0.35 to 29.45±0.45. The percentage of cytomictic cells among the total aberrant cells was observed between 35.19±1.67 and 77.39±1.39. The phytochemical analysis of the plant extract revealed the presence of active secondary metabolites.
Conclusion: The synergistic action of the active compounds might have triggered the phenomenon of cytomixis which, in turn, could be exploited for the production of polyploids.
NOVEL PHARMACEUTICAL COCRYSTAL OF TELMISARTAN AND HYDROCHLOROTHIAZIDE
Authors: KULKARNI ALPANA , SHETE SWAPNIL , HOL VISHAL , BACHHAV RITESH 
Published: March 2020

Objective: Telmisartan (TEL), commonly used antihypertensive, is poorly soluble in water and has limited and variable bioavailability. Commercially, TEL is available as a single drug and in combination with hydrochlorothiazide (HYZ). Researchers have developed cocrystals of TEL with coformers, namely, oxalic acid, glutaric acid, and saccharin. An attempt was made to prepare the cocrystals of TEL with HYZ, an active pharmaceutical ingredient (API) itself so that both the APIs are available in a single tablet. The present study was aimed at enhancement in solubility of TEL by formation of its cocrystals.
Methods: The cocrystals of TEL with HYZ, in different stoichiometric ratios (1:0.5, 1:1, and 1:2), were prepared by solvent coevaporation and liquid-assisted grinding methods. The cocrystals, consisting of TEL:HYZ (in 1:0.5 ratio and 1:1 ratio), depicted maximum yield, drug content, saturation solubility, and flow properties. These cocrystals were characterized by X-ray analysis, infrared spectroscopy, and thermal analysis.
Results: The crystal structure of TEL-HYX revealed that it was a cocrystal, since no proton was transferred between the TEL and HYZ molecules. It was predicted that two molecules are associated through a hydrogen bond between an acidic group of TEL and sulfonamido group of HYZ. The cocrystallization improved the solubility of TEL 7 times. In vitro release rate of tablets of cocrystals was higher than that of marketed TEL tablets. HYZ has a potential to form the cocrystals of TEL.
Conclusion: The objective of improvement in the solubility of TEL was successfully achieved by the formation of cocrystals of TEL: HYZ.
EXTRACTION AND CHARACTERIZATION OF ANTIBACTERIAL PIGMENT FROM ROSEOMONAS GILARDII YP1 STRAIN IN YERCAUD SOIL
Authors: N SIDDHARTHAN , R SANDHIYA , N HEMALATHA 
Published: March 2020

Objective: The current study is focused on antibacterial pigment production from Yercaud hills region soil bacteria.
Methods: In this present study, a soil sample was collected from a coffee plantation in Yercaud, Salem district. The collected soil sample was used to isolate the pigment-producing bacterial strains. The pigments were extracted by acidified ethanol and there used to screen the antibacterial activity against clinical pathogens. The potential antibacterial pigment producer was identifying and the pigment was characterized by ultraviolet spectroscopy, thin-layer chromatography, and Fourier transform infrared (FT-IR) and also evaluate the minimum inhibitory concentration of pigment against clinical pathogens.
Results: The pigment-producing bacterial strains were isolated from Yercaud soil, among the isolates, YP1 was maximum activity against test pathogens. The potential pigment producer was identified as Roseomonas gilardii. The extracted pigments showed the maximum absorbance at 450 nm and their functional groups were identified using FT-IR analysis.
Conclusion: Based on the results, the pigment was extracted from R. gilardii and it may serve as potential antibacterial pigment of interest in the food industries, textile industries in natural colorant, and cosmetic industries.
HIGH RATIO OF MONOSIT: HIGH-DENSITY LIPOPROTEIN AS A RISK FACTOR OFCHRONIC TOTAL OCCLUSION IN PATIENTS CORONARY ARTERY DISEASE
Authors: SURYA I KETUT RADITYA , WITA I WAYAN , ISWARI IDA SRI , RINA ARTHA I MADE JUNIOR , SUNDARI LUH PUTU RATNA 
Published: March 2020

Objective: Chronic total occlusion (CTO) is frequently found in coronary heart disease (CHD) with multivessel lesions. Patients with CTO are associated with an increased mortality rate. Management CTO requires specialized techniques, more resource utilization, and high cost. The progression of atherosclerosis can be prevented by controlling for risk factors. Monocyte to high-density lipoprotein ratio (MHR) is one of the new biomarkers used to assess the incidence of a major adverse cardiovascular event, the severity of coronary lesions, and the incidence of in-stent restenosis. This study was aimed to determine the role of MHR levels as a risk factor for the occurrence of CTO in CAD patients.
Methods: This research is a matched case–control located in Sanglah General Hospital from August 2017 until October 2017. This research consisted of 47 cases with CTO and 47 control without CTO. Case and control samples were adjusted by sex, age, and number of blood vessels involved in CAD.
Results: The cutoff value of MHR was also determined by analyzing the receiver operating curve. The optimal cut off point was 14.33 with sensitivity 83% and specificity 80.9%. Bivariate analysis showed high MHR was found strongly associated with the risk of CTO in CHD patients with odds ratio (OR)=61; IK 95% (OR)=3.211–114.15; p≤0.001. Multivariate analysis showed that high levels of MHR were significantly associated with risk of CTO after other traditional risk factors such as hypertension, diabetes mellitus, dyslipidemia, and smoking were controlled with OR=20,306; 95% IK (OR)=6.05–68.05; p≤0.001.
Conclusion: High levels of MHR were significantly associated with the risk factor of CTO in CAD patients.
Discurso del Cuarto Informe de Actividades del Director de la Facultad de Derecho.
Authors: Contreras Bustamante Raúl 
Published: March 2020

Discurso que con motivo del Cuarto Informe de Actividades rinde ante la Comunidad Académica el Director de la Facultad de Derecho de la UNAM, Dr. Raúl Contreras Bustamante, el día 13 de Febrero del presente año, el el Auditorio Alfonso Caso, Universidad Nacional Autónoma de México.
Pengaruh Peraturan Bank Indonesia Tentang Kredit UMKM Terhadap Total Kredit, Rasio Kredit Umkm, Risiko, Efisiensi dan Profitabilitas Bank Pembangunan Daerah
Authors: Santosa Bagus , Yanuar Yanuar 
Published: March 2020

This research aims to analyze the effect of Bank of Indonesia Regulation on Micro, Small, and Medium Enterprises (MSME) loans towards the Loan Disbursed, MSME Loan Ratio, Risk, Efficiency, and Profitability of Regional Development Banks. This research analyzes the financial data from 10 BPDs over 10 years period to understand the Loan Disbursed, MSME Loan Ratio, Risk, Efficiency, and Profitability of the BPDs before and after the implementation of Regulation Number 14/22/PBI/2012. The financial data were analyzed using the Paired-Sample t-test. Findings show that the overall loan disbursed by BPDs in the period of 2013-2017 or after the Regulation on MSME loans was implemented, is higher than that in the period of 2008-2012 or before the regulation was implemented. However, while the regulation aims to increase the MSME loan disbursed by the banks, the MSME Loan Ratio in the period of 2013-2017 was lower than that in the period of 2008-2012. The Risk, measured by Non-Performing Loan, in the period of 2013-2017 was also increased. The regulation also affects the profitability of the BPDs negatively. The Efficiency, measured by Operating Expenses Operating Income, was not significantly affected by the regulation. The results may inform the regulators to assist the BPDs in responding to the regulation so it could affect BPDs in a more positive way. This may also inform the banks on their financial conditions so they can plan for strategic approaches and develop appropriate schemes on disbursing MSME loans.
Pengaruh Pelayanan Kapal dan Barang Terhadap Kinerja Produktivitas Bongkar Muat Pelabuhan Sunda Kelapa
Authors: Malisan Johny 
Published: March 2020

Salah satu keberhasilan peningkatan pelayanan kapal adalah adanya perbaikan terhadap kinerja pelabuhan sehingga dapat memberikan kepuasan bagi pengguna jasa. Permasalahan pokok dalam pelayanan jasa transportasi laut di Sunda Kelapa adalah kurang efisien dan efektif dalam penyelenggaraannya yang berakibat pada lemahnya daya saing pelabuhan pada tataran nasional khususnya bagi pelabuhan Sunda Kelapa. Hal ini tercermin pada perolehan muatan yang dapat ditangani sebesar ± 3,4 % dari total muatan yang diangkut oleh pelayaran nasional. Kecilnya pangsa muatan ini tentu erat kaitannya dengan produktivitas bongkar muat barang di pelabuhan yang tampaknya belum signifikan oleh karena produktivitas bongkar muat kapal (TSHP) menurun akibat menurunnya kepercayaan pemilik barang yang ditandai dengan mulai beralih pengangkutannya ke jenis kapal konvensional lain. Disamping itu, ship output (TSHP) sebagai indikator produktivitas kapal menunjukkan angka yang kurang baik oleh karena masih banyaknya waktu yang tidak produktif. Hal ini ditunjukkan bahwa TSHP yang pada tahun 2000 sudah tinggi, namun setelah itu cenderung menurun. Demikian pula jam menganggur di pelabuhan sangat besar jika dibandingkan dengan jam efektifnya, dan berdampak pada produktivitas kapal tidak linier dengan kunjungan kapal yang relatif cukup banyak.
ĐÁNH GIÁ KHẢ NĂNG KHÁNG KHUẨN CỦA HỆ VẬT LIỆU NANO TỔ HỢP MANG KHÁNG SINH ĐỐI VỚI VI KHUẨN VIBRIO PARAHAEMOLYTICUS – GÂY BỆNH HOẠI TỬ GAN TUY TỤY CẤP (AHPNS) TRÊN TÔM CHÂN TRẮNG Litopenaeus vannamei (Boone 1931)
Authors: Bình Mạc Như , Anh Lê Thị Kim , Thảo Trần Nguyên , Thịnh Nguyễn Hữu , Thủy Nguyễn Thị Thanh , Nhi Hoàng Thị Yến , Thu Hà Phương , Kim Đặng Đình 
Published: March 2020

TÓM TẮT Nghiên cứu này nhằm mục đích đánh giá khả năng kháng khuẩn của hệ vật liệu nano tổ hợp mang kháng sinh Ag-TiO2-Doxycycline-Alginate (TiO2 - Ag/ DO /Alg) đối với vi khuẩn Vibrio parahaemolyticus - tác nhân chính gây bệnh hoại tử gan tụy cấp trên tôm Chân trắng. Trong nghiên cứu này, hệ vật liệu nano TiO2- Ag/ DO /Alg được tổng hợp tại Viện Khoa học Vật liệu – Viện Hàn Lâm Khoa học và Công nghệ Việt Nam. Chủng vi khuẩn Vibrio parahaemolyticus được phân lập từ 60 mẫu tôm bệnh trên cơ sở triệu chứng bệnh, đặc điểm hình thái và đặc điểm sinh thái. Kết quả thử nghiệm cho thấy hệ nano TiO2-Ag /DO/Alg có hiệu lực diệt khuẩn V. parahaemolyticus tốt và vượt trội hơn kháng sinh DO thông thường (p<0.05). Hệ nano với nồng độ 50ppm cho đường kính vòng kháng khuẩn lớn hơn so với kháng sinh DO ở nồng độ 1000ppm (p<0.05).Từ khóa: TiO2-Ag /DO/Alg, Vibrio parahaemolyticus, bệnh AHPNS
ĐẤU TRANH CHÍNH TRỊ TRONG ĐỒNG KHỞI Ở CÁC TỈNH QUẢNG NAM, QUẢNG NGÃI, BÌNH ĐỊNH VÀ PHÚ YÊN (1964–1965)
Authors: Nhất Phan Thanh 
Published: March 2020

Phong trào Đồng khởi ở nông thôn đồng bằng các tỉnh Khu V là nét nổi bật của cách mạng miền Namnhững năm 1964 - 1965. Trong quá trình lịch sử đó, nét nổi bật trong giải phóng nông thôn đồng bằng các tỉnh Quảng Nam, Quảng Ngãi, Bình Định, Phú Yên là khi lực lượng vũ trang tấn công địch ở những nơi trọng yếu đã hỗ trợ quần chúng nhân dân đứng lên đấu tranh chính trị với nhiều hình thức khác nhau. Các cuộc đấu tranh chính trị đã phát triển thành cao trào, trở thành bạo lực của quần chúng nổi dậy vũ trang phá ấp chiến lược, giải phóng phần lớn nông thôn đồng bằng.