bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Hippo effector TAZ cooperates with oncogenic β-catenin in experimental and human hepatoblastoma development
Authors: Tao Junyan , Zhang Shu , Zhang Jie , Evert Katja , Li Xiaolei , Liu Pin , Kiss Andras , Schaff Zsuzsa , Serra Monica , Evert Matthias , Chen Nianyong , Xu Feng , Chen Xin , Calvisi Diego , Cigliano Antonio 
Published: December 2019

Abstract
Backgrounds: Hepatoblastoma (HB) is the most common pediatric liver tumor. Though Wnt/β-catenin and Hippo cascades are implicated in HB development, there is no study on the crosstalk of β-catenin and Hippo downstream effector TAZ in HB. Methods: The expression of TAZ and of β-catenin in human HB specimens was assessed by immunohistochemistry (IHC). The functional interplay between TAZ and β-catenin was tested through delivering either an activated form of TAZ (TAZS89A) alone or co-delivering TAZS89A and an activated form of β-catenin (∆N90-β-catenin) to mouse liver using sleeping beauty transposase via hydrodynamic tail vein injection (SBT-HTVI). In addition, the role of transcriptional enhanced associate domain (TEAD) factors, canonical Notch cascade, Yap, and the tumor modifier heat shock transcription factor 1 (HSF1) along TAZ/β-catenin-driven HB development was studied in vivo and vitro. Results: Activation of TAZ often co-occurred with that of β-catenin in clinical specimens. While overexpression of TAZS89A alone was unable to promote liver tumorigenesis, the concomitant overexpression of TAZ and ∆N90-β-catenin induced the development of HB lesions exhibiting both epithelial and mesenchymal features. Mechanistically, HB development driven by TAZ/β-catenin required TAZ interaction with TEAD factors. Furthermore, TAZ/β-catenin overexpression induced HB development in conditional Yes-associated protein knockout (Yap KO) mice, indicating that Yap activation is dispensable in this model. Activation of the Notch signaling was observed in TAZ/β-catenin mouse lesions, consistent with that reported in human HBs. Blocking of the canonical Notch cascade using the dominant negative form of RBP-J (dnRBP-J) did not inhibit TAZ/β-catenin dependent HB formation in mice, although suppressed the mesenchymal differentiation. Similarly, upregulation of HSF1 was detected in TAZ/β-catenin lesions, but its inactivation did not affect HB development. In human HB cell lines, silencing of TAZ resulted in decreased cell growth, which was reduced more substantially when TAZ knockdown was associated with suppression of either β-catenin or YAP gene. Conclusions: Overall, our study identifies TAZ as a critical oncogene in HB development and progression. Yap, Notch, and HSF1 are dispensable for TAZ/β-catenin induced HB development in mice.
Laboratory parameters in prognostic sickle cell disease cohort studies in children: a systematic review and lessons for an appropriate strategy for analysis
Authors: Sommet Julie , Roux Enora Le , Koehl Berengere , Haouari Zinedine , Mohamed Damir , Baruchel Andre , Benkerrou Malika , Alberti Corinne 
Published: December 2019

Abstract
BACKGROUND: There is a variability of the results in the published prognostic sickle cell disease (SCD) studies. The aim of this article is to assess and demonstrate how the methods used for analysis of laboratory parameters impact the prognostic study results in children with sickle cell disease. METHODS: Firstly, a systematic review of published studies was conducted to identify the methods used in statistical analyses on laboratory parameters. Secondly, four different identified analysis were successively applied on laboratory data collected prospectively in our cohort of newborns with SCD in order to evaluate the association between laboratory parameters and cerebral vasculopathy, depending on the analysis strategy. RESULTS: Thirty-one studies were included in the systematic review in which hemoglobin and reticulocyte counts were the most frequently collected parameters. Through this review, we noted four different statistical strategies. In almost one half of the cohort studies, laboratory parameter was collected only one time per patient. When several values were available, only one statistical analysis considered the time variation. When we applied these four different statistical strategies to our cohort of SCD children, we highlighted that the conclusion on prognostic value of parameters (either as protective or as risk factor) depends on the analysis strategies applied. CONCLUSION: The laboratory parameter prognostic value depends on the analysis strategy chosen for its analysis. It raises fundamental questions about methodological approaches that concern clinician researchers. Identifying prognostic factors is currently essential for better understanding of SCD. Taking into account longitudinal data and standardization of analyses in prognostic cohort studies should allow drawing valuable and reproducible conclusions.
Depth-related variability in viral communities in highly stratified sulphidic mine tailings
Authors: Gao Shao-Ming , Schippers Axel , Chen Nan , Yuan Yang , Zhang Miao-Miao , Li Qi , Liao Bin , Shu Wen-Sheng , Huang Li-Nan 
Published: December 2019

Abstract
Background: Recent studies have significantly expanded our knowledge of viral diversity and functions in the environment. Exploring the ecological relationships between viruses, hosts and the environment is a crucial first step towards a deeper understanding of the complex and dynamic interplays among them.Results: Here, we obtained extensive 16S rRNA gene amplicon, metagenomics sequencing and geochemical datasets from different depths of two highly stratified sulphidic mine tailings cores with steep geochemical gradients especially pH, and explored how variations in viral community composition and functions were coupled to the co-existing prokaryotic assemblages and the varying environmental conditions. Our data showed that many viruses in the mine tailings represented novel genera, based on gene-sharing networks. Siphoviridae and Myoviridae dominated the classified viruses in the surface tailings and deeper layers, respectively. Both viral richness and normalized coverage increased with depth in the tailings cores and were significantly correlated with geochemical properties, for example, pH. Viral richness was also coupled to prokaryotic richness (Pearson’s r = 0.75, P < 0.01). The enrichment of prophages in the surface mine tailings revealed a preference of lysogenic viral lifestyle in more acidic conditions. Community-wide comparative analyses clearly showed that viruses in the surface tailings acquired genes related to low pH adaptation from archaea while viruses in the deeper layers contained genes mainly annotated as conventional viral functions. Notably, abundant auxiliary sulfate reduction genes were identified from the deeper tailings layers and they were widespread in viruses predicted to infect diverse bacterial phyla.Conclusions: Overall, our results revealed a depth-related distribution of viral populations in the extreme and heterogeneous tailings system. The viruses may interact with diverse hosts and dynamic environmental conditions and likely play a role in the functioning of microbial community and modulate sulfur cycles in situ.
Response to immunomodulatory drug-based regimens in heavily pretreated patients with recurrent/refractory adult Langerhans cell histiocytosis
Authors: Cao Xin-xin , Zhao Ai-lin , Gao Xue-min , Miao Hui-lei , Han Hong-xiao , Niu Na , Li Jian , Zhou Dao-bin , Duan Minghui 
Published: December 2019

Abstract
Purpose: Langerhans cell histiocytosis (LCH) is a clonal histiocytic neoplasm and, because of its rarity in adults, there is no standard treatment for adult LCH. Immunomodulatory drugs (IMiDs) have been used to treat patients with low risk recurrent/refractory LCH but their effectiveness in adult LCH patients is unclear.Methods: We retrospectively evaluated the response rate to IMiDs-based regimens in ten heavily pretreated recurrent/refractory adult LCH patients at Peking Union Medical College Hospital.Results: A total of 10 patients (four males and six females) were included in this study. Median age at diagnosis was 33 years (range, 28–54 years). All patients had multisystem involvement and the median number of organs involved was 5 (range, 5–7). Seven patients had high risk organs involved, including seven patients with liver involvement and one with spleen involvement. All 10 patients had received at least one chemotherapy before the IMiDs-based regimen. The median number of previous lines of chemotherapy was 2 (range, 1–4). Eight patients received thalidomide, dexamethasone and cyclophosphamide, and two patients received lenalidomide and dexamethasone. The median time that patients received thalidomide treatment was 15 months and the duration of the two patients on lenalidomide regimen was 3 months and 12 months separately. Treatment responses in eight recurrent LCH patients included non-active disease in one patient and regressive disease in seven patients. The two refractory patients who had progression during the last treatment had stable disease after IMiDs therapy. During a median 15-months follow-up period, no disease reactivation or death was observed.Conclusions: IMiDs combined with dexamethasone and cyclophosphamide, may be a salvage therapy for recurrent/refractory adult LCH patients.
Influence of pH value on sulfonamide ozonation using caffeine as a contamination indicator
Authors: Gireli Glenda Araujo S. , Maniero Milena Guedes , Guimarães José Roberto 
Published: December 2019

Abstract
Ozonation was effective in degrading six sulfonamides: sulfaquinoxaline, sulfamethazine, sulfamethoxazole, sulfathiazole, sulfadimethoxine, and sulfadiazine (C0 = 100 μg L−1). The degradation experiments were based on two variables: pH (3.0, 7.0 and 11.0) and aqueous matrix (ultrapure water, tap water, and surface water). In all matrices studied and pH 11.0, more than 99% of the drugs were degraded with the highest applied ozone dose (65 mg L−1), when compared with other pH values. Also, comparing the three aqueous matrices, the ultrapure water was the one that required the lowest ozone dosage (6.4 mg L−1) to reach the same degradation efficiency of the sulfonamides. Caffeine is known as an environment contamination indicator. Therefore, this does not intend to totally remove this compound, but to verify its degradation level compared with the sulfonamides.
In Silico Studies for Bacterystic Evaluation against Staphylococcus aureus of 2-Naphthoic Acid Analogues
Authors: Monteiro Alex France Messias , Scotti Marcus Tullius , Speck-Planche Alejandro , Barros Renata Priscila Costa , Scotti Luciana 
Published: December 2019

Background:
Staphylococcus aureus is a gram-positive spherical bacterium commonly present in
nasal fossae and in the skin of healthy people; however, in high quantities, it can lead to complications that
compromise health. The pathologies involved include simple infections, such as folliculitis, acne, and delay in
the process of wound healing, as well as serious infections in the CNS, meninges, lung, heart, and other areas.

Aim:
This research aims to propose a series of molecules derived from 2-naphthoic acid as a bioactive in the
fight against S. aureus bacteria through in silico studies using molecular modeling tools.

Methods:
A virtual screening of analogues was done in consideration of the results that showed activity according
to the prediction model performed in the KNIME Analytics Platform 3.6, violations of the Lipinski
rule, absorption rate, cytotoxicity risks, energy of binder-receptor interaction through molecular docking, and
the stability of the best profile ligands in the active site of the proteins used (PDB ID 4DXD and 4WVG).

Results:
Seven of the 48 analogues analyzed showed promising results for bactericidal action against S.
aureus.

Conclusion:
It is possible to conclude that ten of the 48 compounds derived from 2-naphthoic acid presented
activity based on the prediction model generated, of which seven presented no toxicity and up to one violation
to the Lipinski rule.
Medicinal Plants and Gyneco-obstetric Disorders among Women in the South East of Morocco
Authors: Eddouks Mohamed , Hebi Morad , Ajebli Mohammed 
Published: December 2019

Background:Phytotherapy is used in developing countries to treat several gynecoobstetric disorders.Objective:This study aimed to collect information on herbal remedies traditionally used for the treatment of gyneco-obstetric disorders in the southeast region of Morocco (Tafilalet).Methods:Field work was undertaken as an ethnopharmacological survey involving 980 women, 60 herbalists and 20 midwives in 6 different areas of the Tafilalet region. Data was compiled using a semi-structured questionnaire via face-to-face interviews. Three quantitative ethnopharmacological indices (the use value (UV), informant consensus factor (ICF) and fidelity levels (Fl)) were calculated.Results:A total of 115 species of plants, belonging to 47 families were identified in this study. The most important species according to their use value were Rosmarinus officinalis (0.40), Lavandula angustifolia (0.35), Origanum vulgare (0.22), Cinnamomum cassia (0.18) and Phoenix dactylifera (0.15). Among the obstetric disorders, the highest ICF values were recorded for menstruation (0.95), female infertility (0.9), and maintenance of pregnancy as well as well-being of fetus (0.87). The analysis showed that the highest Fl value for menstruation was found for Origanum vulgare. (94.07 %) followed by Rosmarinus officinalis (87.29%). For female fertility, the highest Fl value was found in Phoenix dactylifera (77.50%) followed by Lepidium sativum (77.17%).Conclusion:This explorative survey emphasizes the need to preserve and document the traditional healing practices for managing gyneco-obstetric disorders and the valorization of this potential could be important for the improvement of women reproductive health.
Study on the Interaction of 1,5-diaryl Pyrrole Derivatives with α-glucosidase; Synthesis, Molecular Docking, and Kinetic Study
Authors: Tafesse Tadesse Bekele , Moghadam Ebrahim Saeedian , Bule Mohammed Hussen , Faramarzi Mohammad Ali , Abdollahi Mohammad , Amini Mohsen 
Published: December 2019

Background:
The delaying of absorption of glucose is one of the principal therapeutic
approaches of type 2 diabetes. α-glucosidase inhibitors compete with the α-glucosidase enzyme activity,
which helps to reduce the conversion of carbohydrates into glucose and thereby control the
postprandial hyperglycemia incidence.

Objective:
The aim of this study was to synthesize a series of novel 1,5-diphenyl pyrrole derivatives
and evaluate their in vitro α-glucosidase inhibitory activities.

Methods:
Compounds were synthesized through a multistep reaction and were evaluated for α-
glucosidase inhibitory activities. Molecular docking and kinetic studies were carried out to predict
the mode of binding and mechanism of inhibition for the most active compounds, 5g and 5b,
against α-glucosidase.

Results:
Synthesized compounds showed good in vitro α-glucosidase inhibitory activity with IC50
values in the range of (117.5 ± 3.8 to 426.0 ± 10.2 μM) as compared to acarbose, the standard
drug, (750 ± 8.7 μM). Compound 5g (117.5 ± 3.8 μM) ascertained as the most potent inhibitor of
α-glucosidase in a competitive mode. The binding energies of compounds 5g and 5b (119.0 ± 7.5
μM), as observed from the best docking conformations, indicate that they have a lower free binding
energy (-3.26 kcal/mol and -3.0 kcal/mol, respectively) than acarbose (2.47 kcal/mol).

Conclusion:
The results of our study revealed that the synthesized compounds are a potential candidate
for α-glucosidase inhibitors for the management of postprandial hyperglycemia for further
investigation.
UPAYA PENINGKATAN PEMBELAJARAN LOMPAT JAUH GAYA MENGGANTUNG DENGAN METODE BAGIAN PROGRESIF SISWA SMAN 1 CEPER
Authors: Hidayat Mufti , Tomoliyus Tomoliyus 
Published: December 2019

Penelitian ini bertujuan untuk meningkatkan pembelajaran teknik lompat jauh gaya menggantung siswa kelas X F SMAN 1 Ceper dengan metode bagian progresif. Jenis penelitian ini adalah penelitian tindakan kelas. Penelitian tindakan adalah kajian sistematik dari upaya perbaikan pelaksanaan praktik pendidikan oleh sekelompok guru dengan melakukan tindakan-tindakan dalam pembelajaran, berdasarkan refleksi mengenai hasil dari tindakan-tindakan tersebut. Penelitian ini dilaksanakan di SMAN 1 Ceper Klaten semester genap tahun pelajaran 2011/2012. Subjek penelitian adalah kelas X F dengan jumlah siswa 23 anak. Penelitian ini dilaksanakan pada bulan Maret-Mei 2012. Hasil analisis penilaian terhadap siswa-siswa kelas X F SMAN 1 Ceper pada cabang atletik lompat jauh gaya menggantung pada semester gasal tahun pelajaran 2011/2012, bahwa mayoritas siswa mengalami kesulitan melakukan tolakan. Hasil penelitian menunjukkan belum terjadi peningkatan hasil belajar siswa rata-rata skor pada siklus I adalah 68,75. Pada siklus II terjadi peningkatan hasil belajar siswa rata-rata skor adalah 80,97. IMPROVING THE LEARNING OF HANG STYLE LONG JUMP WITH PROGRESSIVE PART METHOD OF STUDENTS AT SMAN 1 CEPERAbstract This research is aimed to improve the implementation of hang style long jump technique with progressive part method of class XF students at SMAN 1 Ceper The type of the research is classroom action research. Classroom action research is a systematic study of improving the educational practice implementation by a group of teachers to perform actions in learning, based on their reflections on the results of the actions. This study was conducted at SMAN 1 Ceper Klaten which was in the even semester of 2011/2012 academic year and the research subject was class XF consisting of 23 students. The research was conducted from March to May 2012. Based on the assessment analysis result of class X students SMAN 1 Ceper’ on athletic hang style long jump in the even semester of 2011/2012 academic year, the majority of the students have difficulty in doing repulsion. The research result shows that there is not yet an increase of the students' learning result, the average score in Cycle I is 68.75. In Cycle II there is an increase of the students' learning result, the average score is 80.97 Keywords: hang style long jump, progressive part method
Compliance and Retention With the Experience Sampling Method Over the Continuum of Severe Mental Disorders: Meta-Analysis and Recommendations
Authors: Vachon Hugo , Viechtbauer Wolfgang , Rintala Aki , Myin-Germeys Inez 
Published: December 2019

Background
Despite the growing interest in the experience sampling method (ESM) as a data collection tool for mental health research, the absence of methodological guidelines related to its use has resulted in a large heterogeneity of designs. Concomitantly, the potential effects of the design on the response behavior of the participants remain largely unknown.

Objective
The objective of this meta-analysis was to investigate the associations between various sample and design characteristics and the compliance and retention rates of studies using ESM in mental health research.

Methods
ESM studies investigating major depressive disorder, bipolar disorder, and psychotic disorder were considered for inclusion. Besides the compliance and retention rates, a number of sample and design characteristics of the selected studies were collected to assess their potential relationships with the compliance and retention rates. Multilevel random/mixed effects models were used for the analyses.

Results
Compliance and retention rates were lower for studies with a higher proportion of male participants (P<.001) and individuals with a psychotic disorder (P<.001). Compliance was positively associated with the use of a fixed sampling scheme (P=.02), higher incentives (P=.03), higher time intervals between successive evaluations (P=.02), and fewer evaluations per day (P=.008), while no significant associations were observed with regard to the mean age of the sample, the study duration, or other design characteristics.

Conclusions
The findings demonstrate that ESM studies can be carried out in mental health research, but the quality of the data collection depends upon a number of factors related to the design of ESM studies and the samples under study that need to be considered when designing such protocols.
Land Valuation with Analytical Hierarchy Process and Regression Method in Grogol Sub-District, Sukoharjo District
Authors: Oksaping Arga Fondra 
Published: December 2019

Land valuation is a set of processes to determine the value of land plot. In conducting land valuation, it is necessary to considering the factors that affect values of land which caused land values in each region to be different. In order to objective land valuation, it is necessary to analyze the magnitude of factors that influencing the value of land. The Analytical Hierarchy Process (AHP) method is used in this study to analyzing the magnitude value of land in Grogol Sub-district, Sukoharjo District. Factors used are field distance to CBD, field distance to road, field distance to river, field distance to health facilities, field distance to educational facilities, and land use. The data used in this study were obtined from Sukoharjo Land Office, which is the data of sale and purchase transaction in Grogol Sub-district, Sukoharjo District, totaling 178 data from January to December 2016. Transaction data and factors influence land value are analyzed by Regression Method to obtain the best value model in Grogol Subdistrict, Sukoharjo District.
Pewilayahan Komoditas Pertanian Berdasarkan Zona Agroekologi Skala 1: 50.000 Di Kabupaten Batanghari Jambi
Authors: Saidi Busyra Buyung , Suryani Erna 
Published: December 2019

Penelitian pewilayahan komoditas pertanian berdasarkan Zona Agroekologi (ZAE) skala 1:50.000 di Kabupaten Batanghari, bertujuan: mengidentifikasi potensi sumberdaya lahan untuk pengembangan pertanian; menyusun informasi tipe penggunaan lahan untuk sistem pertanian yang tepat; menyusun peta pewilayahan komoditas pertanian berdasarkan ZAE skala 1:50.000. Penelitian dilakukan dengan pendekatan desk study, observasi dan survei. Hasil penelitian menunjukkan bahwa wilayah Batanghari mempunyai tipe iklim tergolong A dengan nilai Q < 14,3%, mempunyai bulan basah (curah hujan > 100 mm/bulan) rata-rata selama 12 bulan. Hasil analisis landform menunjukkan bahwa Kabupaten Batanghari terdiri atas grup Aluvial, Volkan, dan Tektonik/Stuktural. Bentuk wilayah 44,01% merupakan relief bergelombang, dengan lereng 8-15%.. Hasil identifikasi peta tanah dan verifikasi lapangan menunjukkan bahwa kabupaten Batanghari terdiri atas 30 Satuan Peta Tanah, jenis tanah digolongkan ke dalam 4 ordo tanah, yaitu: Histosol, Inceptisols, Ultisols dan Oxisols. Pewilayahan komoditas pertanian ditetapkan dengan 4 sistem pertanian, yaitu sistem pertanian lahan basah untuk tanaman padi sawah, palawija dan sayuran (Zona IV/Wr dan IV/Wrh), pertanian lahan kering tanaman pangan dan hortikultura (Zona IV/Dfh-1, IV/Dfh-2, IV/Dfh-3, dan IV/Dfeh-4); pertanian lahan kering, tanaman tahunan berbasis tanaman pangan (Zona III/Defh-1 dan III/Defh-2), pertanian lahan kering tanaman tahunan/perkebunan (ZonaII/Deh).