bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association of Health Checkups with Health-Related Quality of Life Among Public Servants: A Nationwide Survey in Taiwan
Authors: Shih Dann-Pyng , Kuo Hsien-Wen , Liang Wen-Miin , Lin Ping-Yi , Tseng Po-chang , Wang Jong-Yi 
Published: November 2020

Abstract
Background: Preventive health checkups have gained in importance over the last decade. The association of health checkups and the number of diseases with health-related quality of life (HRQoL), including physical and mental health, remains unclear. We sought to investigate the aforementioned association among Taiwanese public servants.Methods: A cross-sectional survey was conducted using randomized and multistage stratified cluster sampling based on proportional probabilistic sampling. The questionnaires addressed demographics, job characteristics, health behaviors, health status, 3 types of health checkups during the preceding 3 years (government-paid health checkup [GPHC], self-paid health checkup [SPHC], and no health checkup [NOHC]), and physical component summary (PCS) and mental component summary (MCS) scores of the Short-Form Health Survey. In total 11,454 middle-aged public servants were analyzed. A multivariate general linear model (GLM) was used to estimate PCS and MCS scores by using least square means.Results: Health checkup types were associated with a significant difference in PCS scores among the public servants. Scores of PCS and MCS were both significantly higher in the GPHC group than in the NOHC group for those with no chronic diseases (51.20 vs. 50.66 [P = 0.008] and 46.23 vs. 45.58 [P = 0.02], respectively). Compared with the NOHC group, both scores of GPHC and SPHC groups were significantly associated with higher PCS scores for public servants with ≥2 chronic diseases (46.93 vs. 45.13 [P = 0.002] and 46.52 vs. 45.13 [P = 0.009], respectively).Conclusion: In Taiwan, public servants undergoing GPHCs are more likely to report higher PCS scores than are those undergoing SPHCs. It is crucial that encourage periodically using the health checkup to improve health status and HRQoL.
Under fire-simultaneous volatilome and transcriptome analysis unravels fine-scale responses of tansy chemotypes to dual herbivore attack.
Authors: Clancy Mary V. , Haberer Georg , Jud Werner , Niederbacher Bishu , Niederbacher Simon , Senft Matthias , Zytynska Sharon , Weisser Wolfgang W. , Schnitzler Jörg-Peter 
Published: November 2020

Abstract
BackgroundTansy plants (Tanacetum vulgare L.) are known for their high intraspecific chemical variation, especially of volatile organic compounds (VOC) from the terpenoid compound group. These VOCs are closely involved in plant-insect interactions and, when profiled, can be used to classify plants into groups known as chemotypes. Tansy chemotypes have been shown to influence plant-aphid interactions, however, to date no information is available on the response of different tansy chemotypes to simultaneous herbivory by more than one insect species.ResultsUsing a multi-cuvette system, we investigated the responses of five tansy chemotypes to feeding by sucking and/or chewing herbivores (aphids and caterpillars; Metopeurum fuscoviride Stroyan and Spodoptera littoralis Boisduval). Herbivory by caterpillars following aphid infestation led to a plant chemotype-specific change in the patterns of terpenoids stored in trichome hairs and in VOC emissions. The transcriptomic analysis of a plant chemotype represents the first de novo assembly of a transcriptome in tansy and demonstrates priming effects of aphids on a subsequent herbivory. Overall, we show that the five chemotypes do not react in the same way to the two herbivores. As expected, we found that caterpillar feeding increased VOC emissions, however, a priori aphid infestation only led to a further increase in VOC emissions for some chemotypes.ConclusionsWe were able to show that different chemotypes respond to the double herbivore attack in different ways, and that pre-treatment with aphids had a priming effect on plants when they were subsequently exposed to a chewing herbivore. If neighbouring chemotypes in a field population react differently to herbivory/dual herbivory, this could possibly have effects from the individual level to the group level. Individuals of some chemotypes may respond more efficiently to herbivory stress than others, and in a group environment these "louder" chemotypes may affect the local insect community, including the natural enemies of herbivores, and other neighbouring plants.
A clinical study of curative partial breast irradiation for stage I breast cancer using carbon ion radiotherapy
Authors: Karasawa Kumiko , Omatsu Tokuhiko , Shiba Shintaro , Irie Daisuke , Wakatsuki Masaru , Fukuda Shigekazu 
Published: November 2020

Abstract
Background and purpose: Our institute initiated carbon ion radiotherapy research for patients with stage I breast cancer in April 2013. The purpose of this article is to evaluate the treatment outcome of cases treated outside clinical trial up to May 2020.Materials and methods: Eligibility criteria of the patients were having untreated stage I breast cancer and being unsuitable for operation for physical or mental reasons. The irradiated volume was defined as the gross tumor including intraductal components. The dose escalation study was initially conducted four times a week for a total of 52.8 Gy ([Relative Biological Efficacy (RBE)]). After confirming that adverse effects were within acceptable range, the total dose was increased to 60.0 Gy (RBE). Results: Between April 2013 and November 2015, 14 cases were treated. The median follow up period was 61 months. No adverse toxicities were observed except for grade 1 acute skin reaction in 10 cases. The time required from carbon-ion radiotherapy to tumor disappearance was 3 months in 1 case, 6 months in 3 cases, 12 months in 4 cases, and 24 months in 5 cases. The third case developed local recurrence 6 months after radiotherapy. Twelve patients with luminal subtype received 5-year endocrine therapy. Thirteen of 14 tumors have been maintaining complete response with excellent cosmetic results.Conclusions: The time from carbon ion radiotherapy to tumor disappearance was longer than expected, but complete tumor disappearance was observed except for one high-grade case. With careful patient selection, carbon-ion radiotherapy in patients with stage I breast cancer is deemed effective and safe, and further research is recommended.
The Analgesic Efficacy of Ultrasound-guided Transversus Abdominis Plane (TAP) Block Combined With Oral Multimodal Analgesia In Comparison With Oral Multimodal Analgesia After Caesarean Delivery: A Randomized Controlled Trial
Authors: YU YANG , GAO Shenshan , YUEN Vivian Manying , CHOI Wai Siu , XU Xuebing 
Published: November 2020

Abstract
Background: The transversus abdominis plane (TAP) block is used increasingly in parturients after caesarean delivery. This is a randomized controlled trial to evaluate the effectiveness of bilateral single-shot TAP blocks in patients who received multimodal oral analgesia for postoperative pain relief.Methods: Parturients who were scheduled for elective caesarean delivery under spinal anaesthesia were recruited and randomized to receive bilateral single-shot of TAP blocks or placebo in addition to multimodal oral analgesia which consisted of regular tramadol, celecoxib and paracetamol, with oral oxycodone used as rescue for breakthrough pain. Only parturients in TAP group would receive the TAP blocks with an injection of 15ml (0.25%) ropivacaine on each side under aseptic techniques. All the parturients were evaluated for pain or related complications in the first 24 hours after surgery. Primary outcome is the percentage of parturients who required oxycodone as rescue analgesia. Results: Eighty and seventy-nine parturients were allocated to TAP and placebo group respectively. Nine out of 79 (11.4%) parturients in TAP group and fifteen out of 73 (20.5%) parturients in placebo group required oxycodone for breakthrough pain, P = 0.122.Conclusions: Bilateral single-shot of TAP blocks confer little additional benefit when multimodal oral analgesic regimen is used for pain control after caesarean section under spinal anaesthesia.Trial registration:Clinical Trial Registry of China (http://www.chictr.org.cn, ChiCTR-INR-16010130). Retrospective registered on Dec 12, 2016
DArT-seq based SNP analysis of diversity, population structure and linkage disequilibrium among 274 cowpea (Vigna unguiculata L. Walp.) accessions
Authors: Sodedji Frejus Ariel Kpedetin , Agbahoungba Symphorien , Agoyi Eric Echikintho , Kafoutchoni Konoutan Médard , Kim Ho-Youn , Nguetta Simon-Pierre Assanvo , Assogbadjo Achille Ephrem 
Published: November 2020

Abstract
Background: Genetic diversity in a germplasm is crucial for continuous improvement of crop varieties. A panel of 274 cowpea ( Vigna unguiculata L.) accessions of unknown genetic diversity was assembled from diverse sources. This study used 3127 SNP markers, generated with the diversity array technology (DArT), to assess genetic diversity, population structure and linkage disequilibrium (LD) in the assembled germplasm. Results: The population structure analysis inferred three subpopulations within the germplasm, which was confirmed by Neighbour-Joining (NJ) clustering and principal component analysis (PCA). Low genetic distances (0.005 to 0.44) were observed between accessions. Accessions from Africa; West and Central Africa (113 accessions), East and Central Africa (93 accessions), and Asia (53 accessions) were predominant in the germplasm; and distributed across all subpopulations. High fixation indexes (0.48≤F ST ≤0.56) were obtained for the inferred subpopulations. AMOVA revealed a very large contribution of within subpopulations variation to the observed genetic variation in the germplasm. However, the expected heterozygosity (He) was higher than the observed heterozygosity (Ho), indicating high proportion of inbred lines in the germplasm. Linkage Disequilibrium (LD) was observed in the germplasm, which showed a low decay at longer physical distance between markers in the genome. Conclusions: Significant genetic structuration exists in the assembled cowpea germplasm which shows that there is a potential for improvement of the crop. The subgroups consisted mainly of inbred lines which, although from different geographical regions shared alleles in common reflecting high movement of seeds and exchange of germplasm between regions. The presence of linkage disequilibrium in the germplasm paves a way for prospective whole genome-wide association studies in cowpea for quality attributes and important agronomic traits.
Microincision vitrectomy surgery: Experimental visualization and quantification of vitreous contamination
Authors: Machida Yumiko , Nakashizuka Hiroyuki , Shoji Jun , Shimada Hiroyuki 
Published: November 2020

Abstract
Background: To visualize and quantify vitreous contamination following microincision vitrectomy surgery (MIVS) using an experimental vitreous contamination model (EVCM). Methods: Enucleated porcine eyes with fluoresbrite carboxylate microspheres applied to the conjunctival surface were used as a type 1 EVCM. Twenty-five- or 27-gauge (G) trocar cannulas were inserted through the conjunctiva and sclera, followed by the placing and opening of an infusion cannula. These procedures were monitored by an intraocular fiber catheter. Secondly, condensed microspheres were applied to an excised sheet of porcine sclera to serve as type 2 EVCM. Twenty-five- or 27-G trocar cannulas were inserted perpendicularly through the top of the sclera where the condensed microspheres were applied, an infusion cannula was inserted, 0.1 mL of saline solution injected through the infusion cannula, and samples collected. The fluorescence strength of samples was then measured using fluorophotometry. Results: We visually detected fluorescent microspheres in 10/10 eyes with 25-G and 10/10 with 27-G MIVS. In the experimental quantification study, each MIVS gauge value was significantly higher than the control (P<0.01). However, there was no significant difference between 25-G and 27-G MIVS. Conclusions: MIVS carries the risk of introducing contamination directly into the eyes when the trocar cannula is inserted and infusion cannula is opened, even when a 27-G MIVS is used. Our study has shown it is essential that the surgeon be aware of the possibility of introducing contamination from the conjunctiva at all times during MIVS.
Surgical options for symptomatic old osteoporotic vertebral compression fractures: A retrospective study of 238 cases
Authors: Xu Zhengwei , Hao Dingjun , Dong Liang , Yan Liang , He Baorong 
Published: November 2020

Abstract
Background: Symptomatic osteoporotic vertebral compression fractures (OVCF) are increasing, as are acute and chronic pain episodes and progressive spinal deformities. However, there are no clear surgical treatment criteria for patients with these different symptoms. Therefore, this study aims to explore the surgical approaches for the treatment of OVCF with different symptoms and evaluate the feasibility of these surgical approaches.Methods: We retrospectively analyzed 238 symptomatic OVCF patients who entered our hospital from June 2013 to 2016. According to clinical characteristics and imaging examinations, these patients were divided into I-V grades and their corresponding surgical methods were developed. I, old vertebral fracture with no apparent instability, vertebral augmentation; II, old vertebral fracture with local instability, posterior reduction fusion internal fixation; III, old fractures with spinal stenosis, posterior decompression and reduction fusion and internal fixation; IV, old vertebral fracture with kyphosis, posterior osteotomy with internal fixation and fusion; V, a mixture of the above types, posterior osteotomy (decompression) with internal fixation and fusion. Postoperative visual analog score (VAS), oswestry disability index (ODI) scores, sagittal index (SI) and ASIA grades of neurological function were observed.Results: All 238 patients were followed up for 12-38 months, with an average follow-up of 18.5 months. After graded surgery, the VAS score, ODI score, and vertebral sagittal index SI of 238 patients were significantly improved, and the difference between the last follow-up results and the preoperative comparison was statistically significant (P˂0.05). Besides, the postoperative ASIA grades of 16 patients with nerve injury were improved from 14 patients with preoperative grade C, 2 patients with grade D to 4 patients with postoperative grade D and 12 patients with postoperative grade E.Conclusion: In this study, we concluded that graded surgery could better treat symptomatic old OVCF and restore spinal stability. This provides clinical reference and guidance for the treatment of symptomatic old OVCF in the future.
WITHDRAWN: Expression and prognosis analysis of JMJD5 in human cancers
Authors: Li Hui , Li Qun , Jing Hong , Zhao Jianghai , Zhang Hui , Ma Xuhui , Wei Lunshou , Dai Rujiang , Sun Weihong , Suo Zhimin 
Published: November 2020

Abstract
BackgroundJumonjiC (JmjC) domain-containing protein 5 (JMJD5) plays an important role in cancer metabolism. However, the prognostic value of JMJD5 in most human cancers is still unknown. In this study, we aim to investigate the expression and prognostic value of JMJD5, immune cells infiltration, and the correlations among them. MethodsWe performed a detailed cancer vs. normal analysis of JMJD5 mRNA expression via online Tumor Immune Estimation Resource (TIMER). The protein expressions of JMJD5 in various cancers vs. adjacent normal tissues were examined by immunohistochemistry (IHC) of tissue microarray sections (TMAs). Moreover, the Kaplan-Meier Plotter databases were used to evaluate the prognostic values in above cancers. The correlations between JMJD5 expression level and abundances of six immune infiltrating cells (B cells, CD4+ T cells, CD8+ T cells, macrophages, neutrophils and dendritic cells) were explored by TIMER database in breast cancer (BRCA), liver hepatocellular carcinoma (LIHC), lung adenocarcinoma (LUAD), lung squamous cell carcinoma (LUSC) and stomach adenocarcinoma (STAD). The prognostic values of tumor- infiltrating immune cells were also investigated by TIMER in above four cancers. Finally, the COX proportional hazards model was used to investigate the correlations among clinical outcome, the abundance of immune cell infiltrates and the expression of JMJD5 in above four cancer types.ResultsThe expression of JMJD5 was significantly lower in human breast carcinoma (BRCA), cholangiocarcinoma (CHOL), liver hepatocellular carcinoma (LIHC) and lung cancer (LUC) but higher in prostate adenocarcinoma (PRAD) and stomach adenocarcinoma (STAD) comparing to their respective normal tissues. And high expression of JMJD5 has better prognosis only in BRCA, LIHC, LUC but the opposite effect in STAD. JMJD5 expression is significant correlation with the abundance of six immune cells infiltration in above four cancers. Both the BRCA or lung adenocarcinoma (LUAD) patients with abundance of B cell and the STAD patients with low level of macrophage have a better cumulative survival. ConclusionsWe provided novel evidence of JMJD5 as an essential prognostic biomarker in cancers through analyses the correlation of the JMJD5 expression, tumor-infiltrating B cells and macrophages and prognostic value. This study offers new perspectives therapeutic target in BRCA, LUAD and STAD.
The long-term protective effect of tadalafil on spermatogenesis following testicular ischemia-reperfusion injury in a rat model
Authors: Kim Bomi , Lee EunHye , Yoon BoHyun , Chun So Young , Chung Jae-Wook , Ha Yun-Sok , Kim Bum Soo , Yoon Ghil Suk , Choi Jae Young , Song Phil Hyun , Kwon Tae Gyun , Lee Jun Nyung 
Published: November 2020

Abstract
Background

Testicular torsion is a urological emergency in which misdiagnosis and inappropriate treatment can lead to testicular atrophy and male infertility owing to ischemia-reperfusion injury (IRI). Although experimental studies of testicular torsion have been preceded, promising therapeutic agents based on the long-term effect for spermatogenesis have not been identified in testicular ischemia reperfusion injury (IRI) animal model. Tadalafil, one of the phosphodiesterase-5 inhibitors commonly used in the treatment of erectile dysfunction, has recently reported a protective effect against IRI in several organs. In this study, we evaluated the long-term protective effect of tadalafil for spermatogenesis in a rat testicular IRI model.
Methods

Forty-eight adolescent Sprague–Dawley rats were divided into 6 groups (A-F). Sham operation was performed in group A. Group B received surgical 720-degree torsion of the left testis without any medication. Groups C, D, E, and F were operated surgical torsion with tadalafil at varying doses (0.3 mg/kg, 1.0 mg/kg) and durations (single or daily administration for 4 weeks). Detorsion was performed after 3 hour of torsion in all rats except the sham group. Four weeks after operation, both testes were evaluated of spermatogenesis using Johnsen scoring. To evaluate the protective effect of tadalafil against oxidative stress by IRI, malondialdehyde (MDA) and superoxide dismutase (SOD) level were analyzed via ELISA in both testes 4 hour after detorsion in the same experiments as in group A, B, and C.
Results

For the evaluation of spermatogenesis according to doses, the groups with high-dose tadalafil showed a higher Johnsen scores than low-dose counterparts. The groups with daily administration for 4weeks were observed a higher Johnsen scores than those given a single administration. Furthermore, molecular markers (MDA and SOD) related with oxidative stress and histopathologic findings showed remarkable improvement after tadalafil administration.
Conclusion

Tadalafil alleviated long-term deterioration of spermatogenesis and oxidative stress by restoring antioxidant status after testicular IRI rat model. Furthermore, it demonstrated a protective effect against testicular IRI in a time- and dose-dependent manner.
Hyperglycemia, Inflammatory Response and Infarct Size in Obstructive Acute Myocardial Infarction and MINOCA
Authors: Paolisso Pasquale , Foà Alberto , Bergamaschi Luca , Donati Francesco , Fabrizio Michele , Chiti Chiara , Angeli Francesco , Toniolo Sebastiano , Stefanizzi Andrea , Armillotta Matteo , Rucci Paola , Iannopollo Gianmarco , Casella Gianni , Marrozzini Cinzia , Galiè Nazzareno , Pizzi Carmine 
Published: November 2020

Abstract
Background Hyperglycemia has been associated with increased inflammatory indexes and larger infarct sizes in patients with obstructive acute myocardial infarction (obs-AMI). In contrast, no studies have explored these correlations in non-obstructive acute myocardial infarction (MINOCA). We investigated the relationship between hyperglycemia, inflammation and infarct size in a cohort of AMI patients that included MINOCA. Methods Patients with AMI undergoing coronary angiography between 2016 and 2020 were enrolled. The following inflammatory markers were evaluated: C-reactive protein, neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR)and neutrophil-to-platelet ratio (NPR). Myocardial infarct size was measured by peak high sensitivity troponin I (Hs-TnI) levels, left-ventricular-end-diastolic-volume (LVEDV) and left ventricular ejection fraction (LVEF). Results The final study population consisted of 2450 patients with obs-AMI and 239 with MINOCA. Hyperglycemia was more prevalent among obs-AMI cases. In all hyperglycemic patients - obs-AMI and MINOCA - NLR, NPR, and LPR were markedly altered. Hyperglycemic obs-AMI subjects exhibited a higher Hs-TnI, a larger LVEDV and a lower LVEF compared to normoglycemic ones. Conversely, MINOCA patients showed similar myocardial damage, irrespective of glycemia. Conclusions Our data confirm the association of hyperglycemic obs-AMI with elevated inflammatory markers and larger infarct sizes. MINOCA patients exhibited modest myocardial damage, regardless of admission glucose levels.
Recurrence Time Analysis of the 1883 (Mw 8.6 ~ 9.1) Paleo Megathrust Earthquake in West Bengkulu, Sumatra, Indonesia, Using Plate Kinematic Model for Supporting Mitigation Program
Authors: Murjaya Jaya , Karnawati Dwikorita , Pramumijoyo Subagyo , Supriyanto Supriyanto , Yuliatmoko Rahmat Setyo , Supendi Pepen , Rohadi Supriyanto 
Published: November 2020

Abstract
The great paleo-megathrust earthquake occurred in west Bengkulu-Sumatra on November 25, 1833 and it was estimated to have the magnitude of about Mw 8.6–9.1. This earthquake triggered a tsunami that has 3-4 m height in the Bengkulu coastal and its vicinity. The fundamental earthquake parameter was not known exactly, thus it was difficult to conduct the analysis. We tried to analyze it using scaling law relations of earthquake parameter and magnitude scenarios to estimate the recurrence time based on the plate kinematic model to support the long-term mitigation program. The recurrence time was estimated at 179 years, 250 years, and 314 years if the earthquake is assumed to have a dip-slip mechanism, plate slip rate about 7 cm/year, and magnitude scenarios of 8.6, 8.8, and 9.1 respectively. Then, the recurrence time was estimated at 314 years, 438 years, and 550 years respectively if the earthquake is assumed to have a plate slip rate about 4 cm/year. The other estimation was used the strain rate value of about 1.19 x 10-14/s for Sumatra region and the rigidity modulus of 3.1011 dyne/cm2. We found the recurrence estimation of about 161 years (Mw 8.6), 222 years (Mw 8.8), and 220 years (Mw 9.1) for the dip-slip model. Meanwhile, if this study used all rupture models, the recurrence time of the Bengkulu paleo megathrust earthquake would be about 370 years (Mw 8.6), 558 years (Mw 8.8), and 553 years (Mw 9.1).