bluuebunny/crossref_metadata_2025_split huggingface dataset:
Diasporic still life: Midnight at the Dragon Café and the cultural politics of stasis
Authors: August Timothy K 
Published: December 2019

This article revisits and reevaluates the role that “stasis” can play as a literary technique in diasporic Chinese Canadian writing. To these ends I read Chinese Canadian author Judy Fong Bates’s debut novel Midnight at the Dragon Café (2005) as an important and intimate map of the social geography of a small Canadian town that illuminates how diasporic Chinese life is both constructed and constricted by the institution of the Chinese restaurant. I propose that having a narrative of restaurant life that centres around Chinese Canadian waiters and cooks exposes how socioeconomic institutions reproduce dominant social relations by limiting movement and representational possibilities for immigrant populations. In the book, sedentariness is presented alongside the social and political institutions that generate these diasporic subjects, which, I argue, creates a scene of stasis — where diasporic subjects work to achieve an equilibrium between competing cultural regimes. Bates’s book is remarkable insofar as it maps the unevenness brought on by diasporic globality but in a very "fastened" way — showing how the characters’ global outlooks are shrunk and slowly withered away by the small-town space. This article considers, then, what writing about diasporic stasis achieves in an age that is often characterized by global mobility.
Pathological findings in central nervous system demyelination associated with infliximab
Authors: Kalinowska-Lyszczarz Alicja , Fereidan-Esfahani Mahboobeh , Guo Yong , Lucchinetti Claudia F , Tobin W Oliver 
Published: December 2019

Background: Tumor necrosis factor alpha (TNF-alpha) inhibitors, such as infliximab, are commonly used to treat rheumatoid arthritis (RA) and other immune-mediated disorders. Objective: To determine whether infliximab-associated central nervous system (CNS) demyelination can be differentiated from multiple sclerosis (MS). Methods: We present a case of pathologically proven CNS demyelination in a patient treated with infliximab and describe clinical–radiographic–neuropathological findings. Putative mechanisms of TNF-alpha inhibitor-associated CNS demyelination are described. Results and conclusion: Infliximab treatment is associated with CNS inflammatory demyelinating activity, which is histopathologically indistinguishable from MS.
Optical mapping of electromechanics in intact organs
Authors: Nesmith Haley W , Zhang Hanyu , Rogers Jack M 
Published: December 2019

Optical mapping has become a widely used and important method in cardiac electrophysiology. The method typically uses voltage-sensitive fluorescent dyes and high-speed cameras to image propagation of electrical waves. However, signals are highly susceptible to artifact caused by motion of the target organ. Consequently, cardiac optical mapping is traditionally performed in isolated, perfused organs whose contraction has been pharmacologically arrested. This has prevented optical mapping from being used to study interactions between electrical and mechanical motion. However, recently, a number of groups have developed methods to implement cardiac optical mapping in the presence of motion. These methods employ two basic strategies: (1) compensate for motion by measuring it or (2) ratiometry. In ratiometry, two signals are recorded from each site. The signals have differing sensitivity to membrane potential, but common motion artifact, which can be cancelled by taking the ratio of the two signals. Some methods use both of these strategies. Methods that measure motion have the additional advantage that this information can be used to quantify the organ’s mechanical function. Doing so enables combined “electromechanical mapping,” which allows optical study of electromechanical interactions. By allowing recording in the presence of motion, the new methods open the door to optical recording in in-vivo preparations. In addition, it is possible to implement electromechanical optical mapping techniques in organ systems other than the heart. For example, it was recently shown that optical mapping of slow wave propagation in the swine stomach is feasible. Such studies have the potential to uncover new information on the role of dysrhythmic slow wave propagation in gastric motility disorders. Impact statement Electrical and mechanical functions in the heart are bidirectionally coupled, yet are usually studied separately because of the different instrumentation technologies that are used in the two areas. Optical mapping is a powerful and widespread tool for imaging electrical propagation, but has traditionally required mechanical function to be arrested. Recently new methods have been devised that enable optical mapping to be performed in beating hearts and also to simultaneously quantify mechanical function. These new technologies promise to yield new information about electromechanical interactions in normal and pathological settings. They are also beginning to find application in other organ systems such as the gastrointestinal tract where they may provide new insight into motility disorders.
Deconvoluting the diversity of within-host pathogen strains in a multi-locus sequence typing framework
Authors: Gan Guo Liang , Willie Elijah , Chauve Cedric , Chindelevitch Leonid 
Published: December 2019

Abstract
Background
Bacterial pathogens exhibit an impressive amount of genomic diversity. This diversity can be informative of evolutionary adaptations, host-pathogen interactions, and disease transmission patterns. However, capturing this diversity directly from biological samples is challenging.

Results
We introduce a framework for understanding the within-host diversity of a pathogen using multi-locus sequence types (MLST) from whole-genome sequencing (WGS) data. Our approach consists of two stages. First we process each sample individually by assigning it, for each locus in the MLST scheme, a set of alleles and a proportion for each allele. Next, we associate to each sample a set of strain types using the alleles and the strain proportions obtained in the first step. We achieve this by using the smallest possible number of previously unobserved strains across all samples, while using those unobserved strains which are as close to the observed ones as possible, at the same time respecting the allele proportions as closely as possible. We solve both problems using mixed integer linear programming (MILP). Our method performs accurately on simulated data and generates results on a real data set of Borrelia burgdorferi genomes suggesting a high level of diversity for this pathogen.

Conclusions
Our approach can apply to any bacterial pathogen with an MLST scheme, even though we developed it with Borrelia burgdorferi, the etiological agent of Lyme disease, in mind. Our work paves the way for robust strain typing in the presence of within-host heterogeneity, overcoming an essential challenge currently not addressed by any existing methodology for pathogen genomics.
ShiF acts as an auxiliary factor of aerobactin secretion in meningitis Escherichia coli strain S88
Authors: Genuini Mathieu , Bidet Philippe , Benoist Jean-François , Schlemmer Dimitri , Lemaitre Chloé , Birgy André , Bonacorsi Stéphane 
Published: December 2019

Abstract
Background
The neonatal meningitis E. coli (NMEC) strain S88 carries a ColV plasmid named pS88 which is involved in meningeal virulence. Transcriptional analysis of pS88 in human serum revealed a strong upregulation of an ORF of unknown function: shiF, which is adjacent to the operon encoding the siderophore aerobactin. The aim of this work is to investigate the role of shiF in aerobactin production in strain S88.

Results
Study of the prevalence of shiF and aerobactin operon in a collection of 100 extra-intestinal pathogenic E. coli strains (ExPEC) and 50 whole genome-sequenced E. coli strains revealed the colocalization of these two genes for 98% of the aerobactin positive strains. We used Datsenko and Wanner’s method to delete shiF in two S88 mutants. A cross-feeding assay showed that these mutants were able to excrete aerobactin meaning that shiF is dispensable for aerobactin excretion. Our growth assays revealed that the shiF-deleted mutants grew significantly slower than the wild-type strain S88 in iron-depleted medium with a decrease of maximum growth rates of 23 and 28% (p < 0.05). Using Liquid Chromatography-Mass Spectrometry, we identified and quantified siderophores in the supernatants of S88 and its shiF deleted mutants after growth in iron-depleted medium and found that these mutants secreted significantly less aerobactin than S88 (− 52% and - 49%, p < 0.001).

Conclusions
ShiF is physically and functionally linked to aerobactin. It provides an advantage to E. coli S88 under iron-limiting conditions by increasing aerobactin secretion and may thus act as an auxiliary virulence factor.
SPAR – a randomised, placebo-controlled phase II trial of simvastatin in addition to standard chemotherapy and radiation in preoperative treatment for rectal cancer: an AGITG clinical trial
Authors: Jameson Michael B. , Gormly Kirsten , Espinoza David , Hague Wendy , Asghari Gholamreza , Jeffery Grahame Mark , Price Timothy Jay , Karapetis Christos Stelios , Arendse Michael , Armstrong James , Childs John , Frizelle Frank A. , Ngan Sam , Stevenson Andrew , Oostendorp Martinus , Ackland Stephen P. 
Published: December 2019

Abstract
Background
Retrospective studies show improved outcomes in colorectal cancer patients if taking statins, including overall survival, pathological response of rectal cancer to preoperative chemoradiotherapy (pCRT), and reduced acute and late toxicities of pelvic radiation. Major tumour regression following pCRT has strong prognostic significance and can be assessed in vivo using MRI-based tumour regression grading (mrTRG) or after surgery using pathological TRG (pathTRG).

Methods
A double-blind phase 2 trial will randomise 222 patients planned to receive long-course fluoropyrimidine-based pCRT for rectal adenocarcinoma at 18+ sites in New Zealand and Australia. Patients will receive simvastatin 40 mg or placebo daily for 90 days starting 1 week prior to standard pCRT. Pelvic MRI 6 weeks after pCRT will assess mrTRG grading prior to surgery. The primary objective is rates of favourable (grades 1–2) mrTRG following pCRT with simvastatin compared to placebo, considering mrTRG in 4 ordered categories (1, 2, 3, 4–5). Secondary objectives include comparison of: rates of favourable pathTRG in resected tumours; incidence of toxicity; compliance with intended pCRT and trial medication; proportion of patients undergoing surgical resection; cancer outcomes and pathological scores for radiation colitis. Tertiary objectives include: association between mrTRG and pathTRG grouping; inter-observer agreement on mrTRG scoring and pathTRG scoring; studies of T-cell infiltrates in diagnostic biopsies and irradiated resected normal and malignant tissue; and the effect of simvastatin on markers of systemic inflammation (modified Glasgow prognostic score and the neutrophil-lymphocyte ratio). Trial recruitment commenced April 2018.

Discussion
When completed this study will be able to observe meaningful differences in measurable tumour outcome parameters and/or toxicity from simvastatin. A positive result will require a larger RCT to confirm and validate the merit of statins in the preoperative management of rectal cancer. Such a finding could also lead to studies of statins in conjunction with chemoradiation in a range of other malignancies, as well as further exploration of possible mechanisms of action and interaction of statins with both radiation and chemotherapy. The translational substudies undertaken with this trial will provisionally explore some of these possible mechanisms, and the tissue and data can be made available for further investigations.

Trial registration
ANZ Clinical Trials Register ACTRN12617001087347.
(www.anzctr.org.au, registered 26/7/2017)
Protocol Version: 1.1 (June 2017).
Increased songbird nest depredation due to Aleppo pine (Pinus halepensis) encroachment in Mediterranean shrubland
Authors: Ben-David Asaf , Shamon Hila , Izhaki Ido , Efronny Ronny , Maor Roi , Dayan Tamar 
Published: December 2019

AbstractBackgroundIn recent decades, a decrease of passerine densities was documented in Mediterranean shrublands. At the same time, a widespread encroachment of Aleppo pines (Pinus halepensis) to Mediterranean shrubland occurred. Such changes in vegetation structure may affect passerine predator assemblage and densities, and in turn impact passerine densities. Depredation during the nesting season is an important factor to influence passerine population size. Understanding the effects of changes in vegetation structure (pine encroachment) on passerine nesting success is the main objective of this study. We do so by assessing the effects of Aleppo pine encroachment on Sardinian warbler (Sylvia melanocephala) nest depredation in Mediterranean shrublands. We examined direct and indirect predation pressures through a gradients of pine density, using four methods: (1) placing dummy nests; (2) acoustic monitoring of mobbing events; (3) direct observations on nest predation using cameras; and (4) observation of Eurasian jay (Garrulus glandarius) behaviour as indirect evidence of predation risk.ResultsWe found that Aleppo pine encroachment to Mediterranean shrublands increased nest predation by Eurasian jays. Nest predation was highest in mixed shrubland and pines. These areas are suitable for warblers but had high occurrence rate of Eurasian jays.ConclusionsEncroaching pines directly increase activity of Eurasian jays in shrubland habitats, which reduced the nesting success of Sardinian warblers. These findings are supported by multiple methodologies, illustrating different predation pressures along a gradient of pine densities in natural shrublands. Management of Aleppo pine seedlings and removal of unwanted trees in natural shrubland might mitigate arrival and expansion of predators and decrease the predation pressure on passerine nests.
Region-specific blood–brain barrier transporter changes leads to increased sensitivity to amisulpride in Alzheimer’s disease
Authors: Sekhar Gayathri Nair , Fleckney Alice L. , Boyanova Sevda Tomova , Rupawala Huzefa , Lo Rachel , Wang Hao , Farag Doaa B. , Rahman Khondaker Miraz , Broadstock Martin , Reeves Suzanne , Thomas Sarah Ann 
Published: December 2019

Abstract
Background
Research into amisulpride use in Alzheimer’s disease (AD) implicates blood–brain barrier (BBB) dysfunction in antipsychotic sensitivity. Research into BBB transporters has been mainly directed towards the ABC superfamily, however, solute carrier (SLC) function in AD has not been widely studied. This study tests the hypothesis that transporters for organic cations contribute to the BBB delivery of the antipsychotics (amisulpride and haloperidol) and is disrupted in AD.

Methods
The accumulation of [3H]amisulpride (3.7–7.7 nM) and [3H]haloperidol (10 nM) in human (hCMEC/D3) and mouse (bEnd.3) brain endothelial cell lines was explored. Computational approaches examined molecular level interactions of both drugs with the SLC transporters [organic cation transporter 1 (OCT1), plasma membrane monoamine transporter (PMAT) and multi-drug and toxic compound extrusion proteins (MATE1)] and amisulpride with the ABC transporter (P-glycoprotein). The distribution of [3H]amisulpride in wildtype and 3×transgenic AD mice was examined using in situ brain perfusion experiments. Western blots determined transporter expression in mouse and human brain capillaries .

Results
In vitro BBB and in silico transporter studies indicated that [3H]amisulpride and [3H]haloperidol were transported by the influx transporter, OCT1, and efflux transporters MATE1 and PMAT. Amisulpride did not have a strong interaction with OCTN1, OCTN2, P-gp, BCRP or MRP and could not be described as a substrate for these transporters. Amisulpride brain uptake was increased in AD mice compared to wildtype mice, but vascular space was unaffected. There were no measurable changes in the expression of MATE1, MATE2, PMAT OCT1, OCT2, OCT3, OCTN1, OCTN2 and P-gp in capillaries isolated from whole brain homogenates from the AD mice compared to wildtype mice. Although, PMAT and MATE1 expression was reduced in capillaries obtained from specific human brain regions (i.e. putamen and caudate) from AD cases (Braak stage V–VI) compared to age matched controls (Braak stage 0–II).

Conclusions
Together our research indicates that the increased sensitivity of individuals with Alzheimer’s to amisulpride is related to previously unreported changes in function and expression of SLC transporters at the BBB (in particular PMAT and MATE1). Dose adjustments may be required for drugs that are substrates of these transporters when prescribing for individuals with AD.
Human bone marrow mesenchymal stem cells-derived microRNA-205-containing exosomes impede the progression of prostate cancer through suppression of RHPN2
Authors: Jiang Shuangjian , Mo Chengqiang , Guo Shengjie , Zhuang Jintao , Huang Bin , Mao Xiaopeng 
Published: December 2019

Abstract
Background
Human bone marrow mesenchymal stem cells (hBMSCs) are implicated in cancer initiation and metastasis, sometimes by releasing exosomes that mediate cell communication by delivering microRNAs (miRNAs). This study aimed to investigate the physiological mechanisms by which exosomal miR-205 derived from hBMSCs may modulate the growth of prostate cancer cells.

Methods
Microarray-based gene expression profiling of prostate cancer was adopted to identify differentially expressed genes and regulatory miRNAs, which identified the candidates RHPN2 and miR-205 as the study focus. Then the binding affinity between miR-205 and RHPN2 was identified using in silico analysis and luciferase activity detection. Prostate cancer cells were co-cultured with exosomes derived from hBMSCs treated with either miR-205 mimic or miR-205 inhibitor. Subsequently, prostate cancer cell proliferation, invasion, migration, and apoptosis were detected in vitro. The effects of hBMSCs-miR-205 on tumor growth were investigated in vivo.

Results
miR-205 was downregulated, while RHPN2 was upregulated in prostate cancer cells. RHPN2 was a target of miR-205, and upregulated miR-205 inhibited prostate cancer cell proliferation, invasion, and migration and promoted apoptosis by targeting RHPN2. Next, experiments demonstrated that hBMSCs-derived exosomes carrying miR-205 contributed to repressed prostate cancer cell proliferation, invasion, and migration and enhanced apoptosis. Furthermore, in vivo assays confirmed the inhibitory effects of hBMSCs-derived exosomal miR-205 on prostate cancer.

Conclusion
The hBMSCs-derived exosomal miR-205 retards prostate cancer progression by inhibiting RHPN2, suggesting that miR-205 may present a predictor and potential therapeutic target for prostate cancer.
Systematic underestimation of the epigenetic clock and age acceleration in older subjects
Authors: El Khoury Louis Y. , Gorrie-Stone Tyler , Smart Melissa , Hughes Amanda , Bao Yanchun , Andrayas Alexandria , Burrage Joe , Hannon Eilis , Kumari Meena , Mill Jonathan , Schalkwyk Leonard C. 
Published: December 2019

Abstract
Background
The Horvath epigenetic clock is widely used. It predicts age quite well from 353 CpG sites in the DNA methylation profile in unknown samples and has been used to calculate “age acceleration” in various tissues and environments.

Results
The model systematically underestimates age in tissues from older people. This is seen in all examined tissues but most strongly in the cerebellum and is consistently observed in multiple datasets. Age acceleration is thus age-dependent, and this can lead to spurious associations. The current literature includes examples of association tests with age acceleration calculated in a wide variety of ways.

Conclusions
The concept of an epigenetic clock is compelling, but caution should be taken in interpreting associations with age acceleration. Association tests of age acceleration should include age as a covariate.
An efficient method for meteorological nephogram recognition in cloud environment
Authors: Li Tao , Wang Lei , Ren Yongjun , Li Xiang , Xia Jinyue , An Ran 
Published: December 2019

AbstractNephogram could provide important information for meteorological business, and nephogram recognition is a kind of challenge in the meteorological industry. In this paper, a selective neural network ensemble method based on K-means and Hadoop processing technology is proposed. This method combines the neural network, K-means clustering and AdaBoost in cloud environment. The experimental results show that the recognition precision of the method proposed by this paper is higher than that of traditional method in a stand-alone environment.
Adding exercise to usual care in patients with hypertension, type 2 diabetes mellitus and/or cardiovascular disease: a protocol for a systematic review with meta-analysis and trial sequential analysis
Authors: Rijal Anupa , Nielsen Emil Eik , Hemmingsen Bianca , Neupane Dinesh , Gæde Peter Haulund , Olsen Michael Hecht , Jakobsen Janus Christian 
Published: December 2019

Abstract
Background
Hypertension, type 2 diabetes mellitus and cardiovascular disease are among the leading causes of mortality globally. Exercise is one of the commonly recommended interventions/preventions for hypertension, type 2 diabetes mellitus and cardiovascular disease. However, the previous reviews have shown conflicting evidence on the effects of exercise. Our objective is to assess the beneficial and harmful effects of adding exercise to usual care for people with hypertension, type 2 diabetes mellitus and/or cardiovascular disease.

Methods
This protocol for a systematic review was undertaken using the recommendations of The Cochrane Collaboration, the Preferred Reporting Items for Systematic Reviews and Meta-Analysis Protocols (PRISMA-P) and the eight-step assessment procedure suggested by Jakobsen et al. We plan to include all relevant randomised clinical trials and cluster-randomised trials assessing the effects of adding exercise to usual care for people with hypertension, type 2 diabetes mellitus and/or cardiovascular disease. We will search the Cochrane Central Register of Controlled Trials (CENTRAL), Medical Literature Analysis and Retrieval System Online (MEDLINE), Excerpta Medica database (EMBASE), Latin American and Caribbean Health Sciences Literature (LILACS), Science Citation Index Expanded on Web of Science, Chinese Biomedical Literature Database (CBM), China National Knowledge Infrastructure (CNKI), Chinese Science Journal Database (VIP) and BIOSIS. We will systematically assess the risks of random errors using Trial Sequential Analysis as well as risks of bias of all included trials. We will create a ‘Summary of Findings’ table in which we will present our primary and secondary outcomes, and we will assess the quality of evidence using the Grading of Recommendations Assessment, Development and Evaluation (GRADE).

Discussion
The present systematic review will have the potential to aid patients, clinicians and decision-makers recommending exercise and thereby, benefit patients with hypertension, type 2 diabetes mellitus and/or cardiovascular disease.

Systematic review registration
PROSPERO CRD42019142313