bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mapping the nucleolar proteome reveals a spatiotemporal organization related to intrinsic protein disorder
Authors: Stenström Lovisa , Mahdessian Diana , Gnann Christian , Cesnik Anthony J. , Ouyang Wei , Leonetti Manuel D. , Uhlén Mathias , Cuylen-Häring Sara , Thul Peter J. , Lundberg Emma 
Published: January 2020

AbstractThe nucleolus is essential for ribosome biogenesis and is involved in many other cellular functions. We performed a systematic spatiotemporal dissection of the human nucleolar proteome using confocal microscopy. In total, 1,318 nucleolar proteins were identified; 287 were localized to fibrillar components, and 157 were enriched along the nucleoplasmic border, indicating a potential fourth nucleolar subcompartment (nucleoli rim). We found 65 nucleolar proteins (36 uncharacterized) to relocate to the chromosomal periphery during mitosis. Interestingly, we observed temporal partitioning into two recruitment phenotypes: early (prometaphase) and late (after metaphase), suggesting phase-specific functions. We further show that expression of MKI67 is critical for this temporal partitioning. We provide the first proteome-wide analysis of intrinsic protein disorder for the human nucleolus and show that nucleolar proteins in general, and mitotic chromosome proteins in particular, have significantly higher intrinsic disorder level compared to cytosolic proteins. In summary, this study provides a comprehensive and essential resource of spatiotemporal expression data for the nucleolar proteome as part of the Human Protein Atlas.
Endothelial TGF-β signaling instructs smooth muscle development in the cardiac outflow tract
Authors: Boezio Giulia L.M. , Bensimon-Brito Anabela , Piesker Janett , Guenther Stefan , Helker Christian S.M. , Stainier Didier Y.R. 
Published: January 2020

SummaryThe development of the cardiac outflow tract (OFT), which connects the heart to the great arteries, relies on a complex crosstalk between endothelial (ECs) and smooth muscle (SMCs) cells. Defects in OFT development can lead to severe malformations, including aortic aneurysms, which have often been associated with impaired TGF-β signaling. To further investigate the role of TGF-β signaling in OFT formation, we generated zebrafish lacking the type I TGF-β receptor Alk5 and found a strikingly specific dilation of the OFT.alk5mutants also exhibit increased EC numbers, extracellular matrix (ECM) and SMC disorganization. Surprisingly, endothelial-specificalk5overexpression inalk5mutants rescues both endothelial and SMC defects. Furthermore, modulation of the ECM genefibulin-5, a TGF-β target, partially restores OFT morphology and function. These findings reveal a new requirement for endothelial TGF-β signaling in OFT morphogenesis and suggest an important role for the endothelium in the etiology of aortic malformations.
N6-methyladenosine in poly(A) tails stabilizeVSGtranscripts
Authors: Viegas Idalio J. , de Macedo Juan Pereira , De Niz Mariana , Rodrigues João A. , Aresta-Branco Francisco , Jaffrey Samie R. , Figueiredo Luisa M. 
Published: January 2020

SummaryRNA modifications are important regulators of gene expression. InTrypanosoma brucei, transcription is polycistronic and thus most regulation happens post-transcriptionally.N6-methyladenosine (m6A) has been detected in this parasite, but its function remains unknown. Here we show that ∼50% of the m6A is located in the poly(A) tail of the monoallelically expressed Variant Surface Glycoprotein (VSG) transcript. m6A residues are removed from theVSGpoly(A) tail prior to deadenylation and mRNA degradation. Using genetic tools, we identified a 16-mer motif in the 3’UTR ofVSGthat acts as a cis-acting motif required for inclusion of m6A in the poly(A) tail. Removal of this motif from theVSG3’ UTR results in poly(A) tails lacking m6A, rapid deadenylation and mRNA degradation. To our knowledge this is the first identification of an RNA modification in the poly(A) tail of any eukaryote, uncovering a novel post-transcriptional mechanism of gene regulation.
Fingerprinting of hatchery haplotypes by whole-mitogenome sequencing improves genetic studies of masu salmonOncorhynchus masou masou
Authors: Kato-Unoki Yoko , Umemura Keitaro , Tashiro Kosuke 
Published: January 2020

AbstractStocking hatchery fish can lead to disturbance and extinction of the local indigenous population. Stocking similar lineages to the indigenous population makes it difficult to conduct genetic studies to determine conservation units. We considered that, due to the length of the mitogenome, whole-mitogenome analysis might overcome this problem by enabling identification of hatchery haplotypes. Here, to provide basic information for conservation of indigenous masu salmonOncorhynchus masou masou, a commonly stocked fish in the Kase River system, Japan, we used whole-mitogenome analysis of fish to identify hatchery haplotypes in the river and in several hatcheries that might be used for stocking. Whole-mitogenome sequencing clearly identified hatchery haplotypes like fingerprints, identifying the tributaries contaminated with hatchery haplotypes. The results suggest that informal stocking ofO. m. masouhas been performed widely across the Kase River system. Non-hatchery haplotypes mainly belonged to clade I, which was not found in northern Hokkaido Island. Sites without hatchery haplotypes were estimated three, suggesting that these sites are suitable for conservation of the indigenous fish. This study demonstrated that the resolution of the conventional analysis using partial mitogenome is insufficient to distinguish hatchery haplotype from similar lineages. The whole-mitogenome sequences provided accurate information that was not available in the partial sequences, enabling various inferences: e.g., estimation of the origin of stocked fish and circulation of the reared strains. We conclude that whole-mitogenome analysis is useful for the genetic study of this species. The whole-mitogenome data produced will contribute to the conservation, resource management, and further study ofO. m. masou.
DNA polymerase β prevents AID-instigated mutagenic non-canonical mismatch DNA repair
Authors: Bahjat Mahnoush , Stratigopoulou Maria , Pilzecker Bas , van Dam Tijmen P. , Mobach Simon , Bende Richard J. , van Noesel Carel J.M. , Jacobs Heinz , Guikema Jeroen E.J. 
Published: January 2020

ABSTRACTIn B cells, the error-prone repair of activation-induced cytidine deaminase (AID)-induced lesions in immunoglobulin variable genes cause somatic hypermutation (SHM) of antibody genes. Due to clonal selection in the germinal centers (GC) this active mutation process provides the molecular basis for antibody affinity maturation. AID deaminates cytosine (C) to create uracil (U) in DNA. Typically, the short patch base excision repair (spBER) effectively restores genomic U lesions. We here demonstrate that GC B cells actively degrade DNA polymerase β (Polβ), resulting in the inactivation of the gap-filling step of spBER. Consequently, lesions instigated by AID, and likely other base damages, are channeled towards mutagenic non-canonical mismatch repair (mncMMR), responsible for the vast majority of mutations at adenine and thymine (A:T) bases. Apparently, GC B cells prohibit faithful spBER, thereby favoring A:T mutagenesis during SHM. Lastly, our data suggest that the loss of Polβ relates to hypoxia that characterizes the GC microenvironment.
Determination of critical salinity of pitting and uniform corrosion of X60 under CO2-O2coexistence environment
Authors: Dong BaoJun , Liu Wei , Wu Fei , Zhu JiaQi , Wongpat Banthukul , Zhao Yonggang , Fan Yueming , Zhang TianYi 
Published: January 2020

PurposeThe salinity of the oilfield produced water has a significant effect on steel corrosion. The purpose of this paper is to study the influence of salinity on corrosion behavior of X60 steel and it also provides basic for material selection of gas wells with high salinity.Design/methodology/approachThe weight loss experiment was carried out on steel with high temperature and high pressure autoclave. The surface morphology and composition of corrosion scales were studied by means of scanning electron microscopy, energy dispersive spectroscopy and X-ray diffractometry.FindingsThe results show that as salinity increases, the corrosion rate of X60 steel will gradually experience a rapid decline stage and then a slow decline stage. X60 steel is mainly exhibiting uniform corrosion in the first rapid decline stage and pitting corrosion in the second slow decline stage. The increase in salinity reduces gas solubility, which, in turn, changes the morphology and density of the corrosion scales of X60 steel. At low salinity, loose iron oxides generated on the surface of the steel, which poorly protects the substrate. At high salinity, surface of the steel gradually forms protective films. Chloride ions in the saline solution mainly affect the structure of the corrosion scales and initiate pitting corrosion. The increased chloride ions lead to more pitting pits on the surface of steel. The recrystallization of FeCO3in pitting pits causes the corrosion scales to bulge.Originality/valueThe investigation determined the critical concentration of pitting corrosion and uniform corrosion of X60 steel, and the new corrosion mechanism model was presented.
The influence of half-cycle rectified sinusoidal alternating current (AC) on corrosion of X80 pipeline steel in an acid bicarbonate solution
Authors: Li Hailing , Wan Hongxia , Wang Shengrong , Du Cuiwei , Zhang Dawei 
Published: January 2020

Purpose
This paper aims to investigate the effect of alternating current (AC) on corrosion, it is not yet clear about the exact mechanism of the corrosion induced by AC. Previous reports indicated that AC corrosion was similar to the effect of continuous cathodic and anodic polarization on the corrosion process of the metals. Wan et al. studied the effect of negative half-wave AC on stress corrosion cracking behavior and mechanism of X80 pipeline steel in near-neutral solution.

Design/methodology/approach
This study attempted to understand the AC-induced corrosion by imposing the half-cycle AC on the X80 pipeline steel in an acid bicarbonate solution. The AC corrosion mechanism was determined by weight loss and potentiodynamic polarization curve measurements, as well as surface characterization.

Findings
The results show that the positive half-cycle AC accelerated the uniform corrosion in the NaHCO3 solution, the negative half-cycle AC would decrease the uniform corrosion and local corrosion was increased and some Ca and Mg deposited on the surface of X80 steel, so the corrosion rate decreased by negative half-cycle AC. The corrosion product was composed of α-FeOOH under the application of positive half-cycle AC. The oxygen reduction led to a local increase of pH near the electrode surface and led to the formation of α-FeOOH, which enhanced the protectability of corrosion products.

Originality/value
Researchers studied the effect of negative half-wave AC on stress corrosion cracking behavior and mechanism of X80 pipeline steel in near-neutral solution. However, the AC behavior and corrosion mechanism in acid solution are unknown. So to make clear about the corrosion behavior of metals in different polarization states and the mechanism involved, diode technology was used to research the AC corrosion, half-wave AC was applied on the metals after the full-wave rectified.
An improved selective harmonics elimination technique for PV assisted single phase grid-tied PWM inverter
Authors: Santra Subhendu Bikash , Mohanty Subodh Kumar 
Published: January 2020

Purpose
The purpose of this study is to present a new methodology of selective harmonics elimination (SHE) technique suitable for single-phase photovoltaic (PV) tied pulse width modulated (PWM) inverter.

Design/methodology/approach
In the proposed SHE, switching angles for inverter control are determined offline through numerical techniques and stored in a microcontroller memory as a function of modulation index (md). The methodology uses the solution that leads to a lower change of switching angles from the previous modulation index (md) for storing in the processor memory for multiple solutions. This leads to a smaller number of sections when a piecewise mixed model is considered for storing the entire switching angle curve for the online inverter control. The proposed idea is simulated and experimentally validated on a laboratory prototype of PV (500 W) grid-tied PWM inverter. The control environment is then realized in NI c-RIO 9082.

Findings
This proposed technique is suitable for limiting voltage total harmonics distortion (THD) in single-phase PV tied grid connected voltage source inverter (VSI). Moreover, it is found that filter (L-C) size requirement is less.

Originality/value
The proposed SHE with piecewise mixed model technique effectively reduces voltage THD with less filter size (L-C) in a single-phase PV-tied system.
Analytical approach for spoke-type permanent magnet machine including finite permeability of iron core
Authors: Ladghem Chikouche Brahim , Ibtiouen Rachid 
Published: January 2020

Purpose
The purpose of this study is the introduction of finite permeability of ferromagnetic core in analytical approach for slotted spoke-type permanent magnet machine.

Design/methodology/approach
A two-dimensional analytical approach of magnetic field distribution is established for spoke-type permanent magnet machine to calculate the flux density distribution in the middle of airgap. The paper presents an analytical subdomain model accounting for stator slotting effect. The governing equations are obtained from Maxwell’s equations by using vector potential in all regions of the machine, i.e. magnet, airgap, stator slots and rotor/stator yoke. The finite element analysis is used to validate the analytical results.

Findings
It is found that the developed subdomain model including finite permeability of ferromagnetic core is accurate and is applicable for spoke-type permanent magnet machine for no-load and on-load condition. The analytical results are in accurate agreement with the numerical simulation.

Originality/value
Some assumptions and conditions are presented to improve and simplify the analytical method for analyzing the global saturation for spoke-type permanent magnet machine.
Approaches for increasing the lifetime of single-shot coils
Authors: Schubotz Stefan , Nacke Bernard 
Published: January 2020

Purpose
The purpose of this study is now to optimize the coil in a way that its lifetime is increased. In the industry, induction hardening of rotationally symmetrical workpieces in a single-shot process is a common method. Owing to only partial superimposition of the workpiece areas to be heated by the inductor, high power densities are frequently needed there. These lead to local hot spots, amounting to an intensive material stress in the copper and often result in short lifetime of the inductor.

Design/methodology/approach
In this elaboration, some numerically analysed approaches are presented that can be used to reduce mechanical stresses in the single-shot inductor and thus increase the service life.

Findings
It has been found out that changing the coil profiles has a strong influence on its lifetime. Besides that, the heat transfer coefficient between coil and coolant plays a big role and needs to be considered when the coil is dimensioned.

Originality/value
The lifetime of the coil is an important factor and often requested from the customers. Broken coils create a lot of extra costs and can generate downtime. Therefore, many customers that are struggling with thermal fatigue are interested in approaches that prolong the coil lifetime.
Quadrupolarisability extraction for planar metamaterial scatterers via far-field response
Authors: Karamanos Theodosios , Amanatiadis Stamatis A. , Zygiridis Theodoros , Kantartzis Nikolaos V. 
Published: January 2020

Purpose
The majority of first-principle, homogenisation techniques makes use of the dipole terms of a small particle radiation, and, consequently, the respective dipole polarisabilities. This paper aims to take the next step and propose a new systematic technique for extracting the quadrupolarisability of planar metamaterial scatterers.

Design/methodology/approach
Firstly, it is assumed that the particle, under study, can be modelled as a set of dipole and quadrupole moments, and by utilising the respective polarisabilities, the far-field response of the scatterer is calculated. Then, the far-field scattering field of the particle is constructed in terms of the dipole and quadrupole moments, which, in turn, are expressed as a function of the unknown polarisabilities. Finally, the desired polarisabilities are retrieved by a system of equations, which involves numerically derived electric field values at specific positions around the scatterer.

Findings
The quadrupolarisability of planar metamaterial particles is extracted, through an easy to use, yet very accurate and efficient methodology. Moreover, the proposed technique is verified via comprehensive comparisons of consequently computed and simulated total radiated power values, which reveal its advantages and applicability limits. Finally, the total radiation power contribution of each calculated, individual multipole is provided, to further investigate the radiation mechanism of all nano-particles under study.

Originality/value
The initial and most important step of extracting a single quadrupolarisability of a planar realistic nano-particle has been performed, herein, for the first time. The addition of the respective quadrupole in the scattering model, shifts the multipole approximation limit upwards in terms of frequency, and, therefore, nano-particles with quadrupole resonances can, now, be precisely represented via polarisabilities for various metamaterial or metasurface applications.
Cognitive enhancement with licit and illicit stimulants in the Netherlands and Finland: what is the evidence?
Authors: Hupli Aleksi 
Published: January 2020

Purpose
European studies have shown lower prevalence rates of prescription stimulant use for cognitive enhancement, especially among student populations, compared to North America. This difference requires more cross-country research of the various factors involved. To find out whether other parts of the globe are witnessing similar increases in extra-medical stimulant use, and how this might relate to cognitive enhancement, requires empirical study of local contexts. This paper aims to argue that the academic and public discussion on cognitive enhancement should consider the specific country context of drug policy and research and rethink which drugs are included under the term cognitive enhancement drugs.

Design/methodology/approach
This paper offers a general review and a sociological country comparison between the Netherlands and Finland, focusing not only on prescription stimulants used to treat attention deficit hyperactivity disorder but also illicit amphetamines among young adults and methylphenidate use among Dutch and Finnish participants of the Global Drug Survey. This paper emphasises sociocultural perspectives and the importance of context in cognitive enhancement in general as the line between therapeutic and enhancement use can often be blurred. Data is drawn from global, European and national sources, including the International Narcotics Control Board, European Monitoring Centre for Drugs and Drug Addiction and Global Drug Survey.

Findings
There are hardly any national empirical studies done on cognitive enhancement drug use in Finland. On the other hand, there have been studies in the Netherlands showcasing that the use of prescription stimulants and other drugs for enhancement purposes is something that is happening among young people, albeit yet in a relatively small scale. Illicit and licit stimulant use and drug policy action in relation to cognitive enhancement drugs in the two countries varies, emphasising the importance of country context.

Originality/value
Given that cross-country research is scarce, this general review provides one of the first glimpses into cognitive enhancement drug use by comparing the country context and research in Finland, where the phenomenon has not been studied, with the Netherlands, where the topic has received more research and public attention. Further research areas are suggested.
Knowledge management activities and strategic planning capability development
Authors: Hughes Paul , Hodgkinson Ian 
Published: January 2020

PurposeWhile the strategic management literature extols the virtues of engaging in strategic planning for superior performance, how a dynamic strategic planning capability can be developed remains underexplored; a knowledge void addressed by the paper through applying knowledge-based theory.Design/methodology/approachA mail survey was sent to high technology firms randomly sampled from the Kompass Directory of UK businesses. Firms were sampled at the SBU level, given the focus on strategic planning capability.FindingsAn organization’s strategic planning capability derives from extensive information distribution and organizational memory. While learning values is non-significant, symbolic information use degrades the development of a strategic planning capability.Research limitations/implicationsBy investigating the contributory activities that lead to strategic planning capability development, the findings establish how strategic planning materializes in organizations. Further, the differential effects found for knowledge management activities on strategic planning capability development extend empirical studies that suggest knowledge is always a central tenet of strategic planning.Practical implicationsA set of key knowledge activities is identified that managers must address for strategic planning capability development: strategic planning routines and values of search, analysis and assessment should be appropriately informed by investments in knowledge dissemination and memory on a continual basis. Meanwhile, information misuse compromises strategic planning capabilities, and managers must protect against out-of-context or manipulated information from infiltrating into organizational memory.Originality/valueDespite the advent of the knowledge-based theory and its core premise that capabilities derive from knowledge management activities, little research has been conducted into demonstrating the knowledge-based antecedents of a strategic planning capability.
Odd couple collaborations and making them tick!
Authors: Jha Pushkar P. , Johnston Lorraine 
Published: January 2020

Purpose
Be it about blending intangibles to deliver to market needs or directed at fulfilling aspirations pushing at technological frontiers, inter-firm collaborations across industry boundaries are much in vogue. This paper aims to classify some collaborations as “odd couple collaborations”. These are fuelled more by aspirations of the partner firms, and not as much by market pull. The study provides key distinguishing characteristics for these and an understanding of what makes them tick.

Design/methodology/approach
The paper draws on secondary sources in the public domain to understand the motives and performance of several inter-firm collaborations. Odd couple collaborations are examined and some essential performance enablers are highlighted.

Findings
A typology that distinguishes odd couple collaborations from other inter-firm collaborations is drawn out. Analysing the performance of such collaborations, and a need for partners to work on the visibility and appeal of such collaborations, is discussed. Stringent market evaluation of the offering and careful creative blending of intangibles are also highlighted as key enablers.

Originality/value
The paper contributes to a vast body of research on inter-sector or distant collaborations by isolating and examining a niche that is fast becoming pronounced. The analysis of odd couple collaborations provides cues for effective strategies for superior value from such collaborations. As organisations constantly seek to extend their innovative potential, these insights may prove useful for both practice and research.