bluuebunny/crossref_metadata_2025_split huggingface dataset:
Rat epidermal stem cells promote the angiogenesis of full-thickness wounds
Authors: Huang Shaobin , Hu Zhicheng , Wang Peng , Zhang Yi , Cao Xiaoling , Dong Yunxian , Cheng Pu , Xu Hailin , Zhu Wenkai , Tang Bing , Zhu Jiayuan 
Published: August 2020

Abstract
Background
Full-thickness wounds severely affect patients’ life quality and become challenging problems for clinicians. Stem cells have great prospects in the treatment of wounds. Our previous study confirmed that autologous basal cell suspension could promote wound healing, and epidermal stem cells (ESCs) were detected in the basal cell suspension. Herein, this study aimed to explore the effect of ESCs on full-thickness wounds.

Methods
Rat ESCs were isolated and expanded and then were transfected with lentivirus to stably express enhanced green fluorescent protein. The experimental rats were randomly divided into 2 groups: in the ESC group, the rat ESCs were sprayed on the full-thickness wounds of rats; in the control group, phosphate-buffered saline was sprayed the on the wounds. Next, wound healing and neovascularization were evaluated. Colonization, division, and differentiation of ESCs on the wound were analyzed by immunofluorescence.

Results
The rat ESCs colonized, divided, and proliferated in the wound. Additionally, rat ESCs around blood vessels differentiated into vascular endothelial cells and formed a lumen-like structure. Compared with the control group, the ESC group showed enhanced angiogenesis and accelerated wound healing.

Conclusions
Our study confirmed that rat ESCs are safe and effective for treating full-thickness wounds. Additionally, under certain conditions, ESCs can differentiate into vascular endothelial cells to promote angiogenesis and wound healing.
Biological characteristics of endometriotic mesenchymal stem cells isolated from ectopic lesions of patients with endometriosis
Authors: Liu Yanli , Liang Shengying , Yang Fen , Sun Yuliang , Niu Lidan , Ren Yakun , Wang Hongmei , He Yanan , Du Jiang , Yang Jun , Lin Juntang 
Published: August 2020

Abstract
Background
Research into the pathogenesis of endometriosis (EMs) would substantially promote its effective treatment and early diagnosis. However, the aetiology of EMs is poorly understood and controversial despite the progress in EMs research in the last several decades. Currently, accumulating evidence has shed light on the importance of endometrial stem cells (EnSCs) residing in the basal layer of endometrium in the establishment and progression of endometriotic lesions. Therefore, we aimed to identify the differences between EnSCs isolated from the ectopic lesions of EMs patients (EnSC-EM-EC) and EnSCs isolated from eutopic endometrium of control group (EnSC-Control). We further performed preliminary exploration of the potential signalling pathways involved in the above abnormalities.

Methods
EnSC-EM-EC (n = 12) and EnSC-Control (n = 13) were successfully isolated. Then, the proliferative capacity, migratory capacity and angiogenic potential of EnSCs were evaluated by conventional MTT assay, flow cytometry, wound healing assay, transwell assay, tube formation assay and chick embryo chorioallantoic membrane assay respectively. The expression of 11 angiogenesis-associated biological factors and 11 cytokines secreted by EnSCs and 17 adhesion molecules expressed on EnSCs were determined by protein array assays respectively. Differentially expressed genes (DEGs) between EnSC-EM-EC and EnSC-Control were analysed by RNA-sequence.

Results
EnSC-EM-EC exhibited unique biological characteristics, including prolonged mitosis, enhanced migratory capacity and enhanced angiogenic potential. Greater amounts of angiogenic factors (especially VEGF and PDGF) were secreted by EnSC-EM-EC than by EnSC-Control; however, the distinct profiles of cytokines secreted by EnSC-EM-EC and adhesion molecules expressed by EnSC-EM-EC require further investigation. A total of 523 DEGs between EnSC-EM-EC and EnSC-Control were identified and analysed using the KEGG and Gene Ontology databases.

Conclusions
Our results not only improve the understanding of EMs but also contribute to the development of EnSC-EM-EC as a tool for EMs drug discovery. These cells could be of great help in exploiting promising therapeutic targets and new biomarkers for EMs treatment and prognosis.
Dopamine transporter neuroimaging accurately assesses the maturation of dopamine neurons in a preclinical model of Parkinson’s disease
Authors: Goggi Julian L. , Qiu Lifeng , Liao Mei Chih , Khanapur Shivashankar , Jiang Lingfan , Boominathan Ramasamy , Hartimath Siddesh V. , Cheng Peter , Yong Fui Fong , Soh Vanessa , Deng Xiaozhou , Lin Youshan Melissa , Haslop Anna , Tan Peng Wen , Zeng Xiaoxia , Lee Jolene W. L. , Zhang Zhiwei , Sadasivam Pragalath , Tan Eng King , Luthra Sajinder K. , Shingleton William D. , Oh Steve K. W. , Zeng Li , Robins Edward G. 
Published: August 2020

Abstract
Background
Significant developments in stem cell therapy for Parkinson’s disease (PD) have already been achieved; however, methods for reliable assessment of dopamine neuron maturation in vivo are lacking. Establishing the efficacy of new cellular therapies using non-invasive methodologies will be critical for future regulatory approval and application. The current study examines the utility of neuroimaging to characterise the in vivo maturation, innervation and functional dopamine release of transplanted human embryonic stem cell-derived midbrain dopaminergic neurons (hESC-mDAs) in a preclinical model of PD.

Methods
Female NIH RNu rats received a unilateral stereotaxic injection of 6-OHDA into the left medial forebrain bundle to create the PD lesion. hESC-mDA cell and sham transplantations were carried out 1 month post-lesion, with treated animals receiving approximately 4 × 105 cells per transplantation. Behavioural analysis, [18F]FBCTT and [18F]fallypride microPET/CT, was conducted at 1, 3 and 6 months post-transplantation and compared with histological characterisation at 6 months.

Results
PET imaging revealed transplant survival and maturation into functional dopaminergic neurons. [18F]FBCTT-PET/CT dopamine transporter (DAT) imaging demonstrated pre-synaptic restoration and [18F]fallypride-PET/CT indicated functional dopamine release, whilst amphetamine-induced rotation showed significant behavioural recovery. Moreover, histology revealed that the grafted cells matured differently in vivo producing high- and low-tyrosine hydroxylase (TH) expressing cohorts, and only [18F]FBCTT uptake was well correlated with differentiation.

Conclusions
This study provides further evidence for the value of in vivo functional imaging for the assessment of cell therapies and highlights the utility of DAT imaging for the determination of early post-transplant cell maturation and differentiation of hESC-mDAs.
NF-κB signaling in rheumatoid arthritis with focus on fibroblast-like synoviocytes
Authors: Nejatbakhsh Samimi Leila , Farhadi Elham , Tahmasebi Mohammad Naghi , Jamshidi Ahmadreza , Sharafat Vaziri Arash , Mahmoudi Mahdi 
Published: August 2020

AbstractThe nuclear factor-κB (NF-κB) signaling pathway regulates multiple processes in innate and adaptive immune cells. This pathway is involved in inflammation through the regulation of cytokines, chemokines, and adhesion molecules expression. The NF-κB transcription factor also participates in the survival, proliferation, and differentiation of cells. Therefore, deregulated NF-κB activation contributes to the pathogenesis of inflammatory diseases. Rheumatoid arthritis (RA) is classified as a heterogeneous and complex autoimmune inflammatory disease. Although different immune and non-immune cells contribute to the RA pathogenesis, fibroblast-like synoviocytes (FLSs) play a crucial role in disease progression. These cells are altered during the disease and produce inflammatory mediators, including inflammatory cytokines and matrix metalloproteinases, which result in joint and cartilage erosion. Among different cell signaling pathways, it seems that deregulated NF-κB activation is associated with the inflammatory picture of RA. NF-κB activation can also promote the proliferation of RA-FLSs as well as the inhibition of FLS apoptosis that results in hyperplasia in RA synovium. In this review, the role of NF-κB transcription factor in immune and non-immune cells (especially FLSs) that are involved in RA pathogenesis are discussed.
An age and space structured SIR model describing the Covid-19 pandemic
Authors: Colombo Rinaldo M. , Garavello Mauro , Marcellini Francesca , Rossi Elena 
Published: August 2020

AbstractWe present an epidemic model capable of describing key features of the Covid-19 pandemic. While capturing several qualitative properties of the virus spreading, it allows to compute the basic reproduction number, the number of deaths due to the virus and various other statistics. Numerical integrations are used to illustrate the adherence of the evolutions described by the model to specific well known real features of the present pandemic. In particular, this model is consistent with the well known relevance of quarantine, shows the dramatic role of care houses and accounts for the increase in the death toll when spatial movements are not constrained.
Surface display of p75, a Lactobacillus rhamnosus GG derived protein, on Bacillus subtilis spores and its antibacterial activity against Listeria monocytogenes
Authors: Kang Soo Ji , Jun Ji Su , Moon Jeong A , Hong Kwang Won 
Published: August 2020

AbstractLactobacillus rhamnosus p75 protein with peptidoglycan hydrolase (PGH) activity is one of the key molecules exhibiting anti-apoptotic and cell-protective activity for human intestinal epithelial cells. In this study, with the goal of developing new probiotics, the p75 protein was displayed on the surface of Bacillus subtilis spores using spore coat protein CotG as an anchoring motif. The PGH activity, stability, and the antibacterial activity of the spore-displayed p75 (CotG-p75) protein were also investigated. The PGH activity of the CotG-p75 against peptidoglycan extracted from B. subtilis was confirmed by the ninhydrin test. Under various harsh conditions, compared to the control groups, the PGH activities of CotG-p75 were very stable in the range of pH 3–7 and maintained at 70% at 50 °C. In addition, the antibacterial activity of CotG-p75 against Listeria monocytogenes was evaluated by a time-kill assay. After 6 h incubation in phosphate-buffered saline, CotG-p75 reduced the number of viable cells of L. monocytogenes by up to 2.0 log. Scanning electron microscopy analysis showed that the cell wall of L. monocytogenes was partially damaged by the treatment with CotG-p75. Our preliminary results show that CotG-p75 could be a good candidate for further research to develop new genetically engineered probiotics.
Use of powered air-purifying respirator (PAPR) by healthcare workers for preventing highly infectious viral diseases—a systematic review of evidence
Authors: Licina Ana , Silvers Andrew , Stuart Rhonda L. 
Published: August 2020

AbstractBackgroundHealthcare workers (HCWs) are at particular risk during pandemics and epidemics of highly virulent diseases with significant morbidity and case fatality rate. These diseases include severe acute respiratory syndrome coronaviruses, SARS-CoV-1 and SARS-CoV-2, Middle Eastern Respiratory Syndrome (MERS), and Ebola. With the current (SARS-CoV-2) global pandemic, it is critical to delineate appropriate contextual respiratory protection for HCWs. The aim of this systematic review was to evaluate the effect of powered air-purifying respirators (PAPRs) as part of respiratory protection versus another device (egN95/FFP2) on HCW infection rates and contamination.MethodsOur primary outcomes included HCW infection rates with SARS-CoV-2, SARS-CoV-1, Ebola, or MERS when utilizing PAPR. We included randomized controlled trials, non-randomized controlled trials, and observational studies. We searched the following databases: MEDLINE, EMBASE, and Cochrane Library (Cochrane Database of Systematic Reviews and CENTRAL). Two reviewers independently screened all citations, full-text articles, and abstracted data. Due to clinical and methodological heterogeneity, we did not conduct a meta-analysis. Where applicable, we constructed evidence profile (EP) tables for each individual outcome. Confidence in cumulative evidence for each outcome was classified according to the GRADE system.ResultsWe identified 689 studies during literature searches. We included 10 full-text studies. A narrative synthesis was provided. Two on-field studies reported no difference in the rates of healthcare workers performing airway procedures during the care of critical patients with SARS-CoV-2. A single simulation trial reported a lower level of cross-contamination of participants using PAPR compared to alternative respiratory protection. There is moderate quality evidence that PAPR use is associated with greater heat tolerance but lower scores for mobility and communication ability. We identified a trend towards greater self-reported wearer comfort with PAPR technology in low-quality observational simulation studies.ConclusionField observational studies do not indicate a difference in healthcare worker infection utilizing PAPR devices versus other compliant respiratory equipment. Greater heat tolerance accompanied by lower scores of mobility and audibility in PAPR was identified. Further pragmatic studies are needed in order to delineate actual effectiveness and provider satisfaction with PAPR technology.Systematic review registrationThe protocol for this review was prospectively registered with the International Register of Systematic Reviews identification numberCRD42020184724.
A generalized Hölder-type inequalities for measurable operators
Authors: Han Yazhou , Shao Jingjing 
Published: August 2020

AbstractWe prove a generalized Hölder-type inequality for measurable operators associated with a semi-finite von Neumann algebra which is a generalization of the result shown by Bekjan (Positivity 21:113–126, 2017). This also provides a generalization of the unitarily invariant norm inequalities for matrix due to Bhatia–Kittaneh, Horn–Mathisa, Horn–Zhan and Zou under a cohyponormal condition.
The convergence rate of truncated hypersingular integrals generated by the modified Poisson semigroup
Authors: Eryiğit Melih , Evcan Sinem Sezer , Çobanoğlu Selim 
Published: August 2020

AbstractHypersingular integrals have appeared as effective tools for inversion of multidimensional potential-type operators such as Riesz, Bessel, Flett, parabolic potentials, etc. They represent (at least formally) fractional powers of suitable differential operators. In this paper the family of the so-called “truncated hypersingular integral operators” $\mathbf{D}_{\varepsilon }^{\alpha }f$

D
ε
α

f
is introduced, that is generated by the modified Poisson semigroup and associated with the Flett potentials

F
α

φ
=

(
E
+

−
Δ

)

−
α

φ
($0<\alpha <\infty $
0
<
α
<
∞
, $\varphi \in L_{p}(\mathbb{R}^{n})$
φ
∈

L
p

(

R
n

)
). Then the relationship between the order of “$L_{p}$

L
p

-smoothness” of a function f and the “rate of $L_{p}$

L
p

-convergence” of the families $\mathbf{D}_{\varepsilon }^{\alpha } \mathcal{F}^{\alpha }f$

D
ε
α

F
α

f
to the function f as $\varepsilon \rightarrow 0^{+}$
ε
→

0
+

is also obtained.
Commutators of θ-type generalized fractional integrals on non-homogeneous spaces
Authors: Lu Guanghui 
Published: August 2020

AbstractThe aim of this paper is to establish the boundednes of the commutator $[b,T_{\alpha }]$
[
b
,

T
α

]
generated by θ-type generalized fractional integral $T_{\alpha }$

T
α

and $b\in \widetilde{\mathrm{RBMO}}(\mu )$
b
∈

RBMO
˜

(
μ
)
over a non-homogeneous metric measure space. Under the assumption that the dominating function λ satisfies the ϵ-weak reverse doubling condition, the author proves that the commutator $[b,T_{\alpha }]$
[
b
,

T
α

]
is bounded from the Lebesgue space $L^{p}(\mu )$

L
p

(
μ
)
into the space $L^{q}(\mu )$

L
q

(
μ
)
for $\frac{1}{q}=\frac{1}{p}-\alpha $

1
q

=

1
p

−
α
and $\alpha \in (0,1)$
α
∈
(
0
,
1
)
, and bounded from the atomic Hardy space $\widetilde{H}^{1}_{b}(\mu )$

H
˜

b
1

(
μ
)
with discrete coefficient into the space $L^{\frac{1}{1-\alpha },\infty }(\mu )$

L

1

1
−
α

,
∞

(
μ
)
. Furthermore, the boundedness of the commutator $[b,T_{\alpha }]$
[
b
,

T
α

]
on a generalized Morrey space and a Morrey space is also got.
Extragradient-like method for pseudomontone equilibrium problems on Hadamard manifolds
Authors: Chen Junfeng , Liu Sanyang 
Published: August 2020

AbstractThis paper presents an extragradient-like method for solving a pseudomonotone equilibrium problem with a Lipschitz-type condition on Hadamard manifolds. The algorithm only needs to know the existence of the Lipschitz-type constants of the bifunction, and the stepsize of each iteration is determined by the adjacent iterations. Convergence of the algorithm is analyzed, and its application to variational inequalities is also provided. Finally, several experiments are made to verify the effectiveness of the algorithms.
Stakeholders’ satisfaction as a key determinant of critical success factors in renewable energy projects
Authors: Maqbool Rashid , Deng Xiaomei , Rashid Yahya 
Published: August 2020

AbstractRenewable energy projects (REPs) are critical to providing a clean and sustainable environmental perspective alongside the economic prosperity of any country. Unfortunately, recent trends in renewable energy projects (REPs) are not positive enough regarding their successful completion within the budgeted cost, planned time, proposed quality, and other necessary constraints. Although the pertinent literature has discussed the critical success factors (CSFs) for such projects, their influencing mechanism with respect to the role of key stakeholders is still overlooked. This study recognizes several critical success factors (CSFs) and their influencing mechanisms onto renewable energy projects (REPs) in order to evaluate direct influences onto project success as well as indirect influences through stakeholders’ satisfaction (SS) as mediation on the project success of small and medium-sized renewable energy projects (REPs) in Pakistan’s energy sector. A sample of about 272 respondents working with renewable energy projects (REPs), including key stakeholders, has been collected to perform data analysis and draw inferences. A structural equation modeling (SEM) approach was used for data analysis and inference drawing. The results show that though there is a positive and direct association between critical success factors (CSFs) and project success, a partial mediating role of stakeholders’ satisfaction (SS) between CSFs and project success was still determined. Hence, it was found that CSFs significantly contribute to renewable energy projects (REPs) through mediating influences of stakeholders’ satisfaction (SS). The findings confirmed that stakeholders’ satisfaction (SS) is a key determinant for critical success factors (CSFs) in renewable energy projects (REPs). Moreover, stakeholders’ satisfaction (SS) emerged to be a crucial element for the completion of such projects. The empirical findings of this study might have a useful effect on academicians and the practitioners engaged with renewable energy projects (REPs).
High rate of extended-spectrum beta-lactamase-producing gram-negative infections and associated mortality in Ethiopia: a systematic review and meta-analysis
Authors: Tufa Tafese B. , Fuchs Andre , Tufa Takele B. , Stötter Loraine , Kaasch Achim J. , Feldt Torsten , Häussinger Dieter , Mackenzie Colin R. 
Published: August 2020

Abstract
Background
Extended-spectrum beta-lactamase (ESBL)-producing Gram-negative bacteria have become a serious threat to global health. Their rapid spread is associated with high mortality due to ineffective antibiotic treatment. To date a regular surveillance of multidrug-resistant (MDR) pathogens in Ethiopia is not established. For this report, published data regarding ESBL-producing bacteria in different health facilities of Ethiopia were reviewed.

Methods
This study collates data from published information on the rates and clinical implications of infection with ESBL-producing Gram-negative bacteria in Ethiopia. A systematic literature search was conducted using PubMed, PubMed Central, Medline, Science Direct and Google scholar from October 2018 to March 2019. Eligible studies were identified by applying quality criteria. The pooled proportion of ESBL-producing Gram-negative bacteria was estimated based on a random effect model. The publication bias and the variation in proportion estimates attributed to heterogeneity were assessed.

Results
Fourteen studies with relevant data were included in the review. In total, 1649 Gram-negative bacteria isolated from 5191 clinical samples were included. The pooled proportion estimate of ESBL-producing Gram-negative bacteria was 50% (95% CI: 47.7–52.5%. Data showed a high level of heterogeneity (I2 = 95%, P <  0.01). ESBL rates varied by species; 65.7% (263/400) in Klebsiella spp., 48.4% (90/186) in Salmonella spp., and 47.0% (383/815) in E. coli. ESBL-encoding genes were reported in 81 isolates: 67 isolates harbored the CTX-M-1 group and 14 isolates TEM. The mortality associated with infections by bacteria resistant to third generation cephalosporins has rarely been investigated. However, two studies reported a mortality of 33.3% (1/3) and 100% (11/11).

Conclusions
In this meta-analysis, the pooled prevalence of ESBL-producing pathogens is alarmingly high. Data on mortality rates is scarce. This highlights the need for establishing and upgrading clinical microbiology laboratories in Ethiopia for routine antibiotic susceptibility testing and extended surveillance of multidrug resistance.
The effect of eye movement desensitization and reprocessing on the fear of hypoglycemia in type 2 diabetic patients: a randomized clinical trial
Authors: Sheikhi Mohammadreza , Moradi Mohamad , Shahsavary Saeed , Alimoradi Zainab , Salimi Hamid Reza 
Published: August 2020

Abstract
Background
The fear of hypoglycemia leads to psychological symptoms in patients with diabetes type 2. In this research, the effects of EDMR on the fear of hypoglycemia in patients with diabetes type 2 were examined.

Methods
A clinical trial study was carried out with participation of 72 patients who had diabetes type 2 in Velayat Hospital. The participants were randomly assigned into control and intervention groups. The intervention group received EMDR. The required information was gleaned using a questionnaire of fear of hypoglycemia, intensity of hypoglycemia, and demographics filled out before the intervention, and 1 month and 3 months after it. The data were analyzed using descriptive statistics on SPSS Version 23. For comparison of fear of hypoglycemia in intervention and control groups, repeated measure ANOVA and Cohen d test were used.

Results
The mean age of the participants in the intervention group was 43.17 ± 10.55 and in the control group was 45.86 ± 13.6. In this study, without considering the potential disruptors in the incorrect model, the intervention caused a reduction of 15 points 1 month after the completion of the intervention and a reduction of 17 points 3 month after the completion of the intervention on the scale of fear of hypoglycemia; but post-correction of potential disruptors, intervention caused a reduction of 19.5 scores 1 month after the completion and a reduction of 20.3 scores 3 months after the intervention .

Conclusions
The EMDR can be used as a non-pharmaceutical treatment method to treat and alleviate the fear of hypoglycemia in type 2 diabetes patients.

Trial registration
Iranian Registry of Clinical Trials: IRCT20181201041813N1, 2019/11/13.