bluuebunny/crossref_metadata_2025_split huggingface dataset:
ALS motor neurons exhibit hallmark metabolic defects that are rescued by SIRT3 activation
Authors: Hor Jin-Hui , Santosa Munirah Mohamad , Lim Valerie Jing Wen , Ho Beatrice Xuan , Taylor Amy , Khong Zi Jian , Ravits John , Fan Yong , Liou Yih-Cherng , Soh Boon-Seng , Ng Shi-Yan 
Published: November 2020

AbstractMotor neurons (MNs) are highly energetic cells and recent studies suggest that altered energy metabolism precede MN loss in amyotrophic lateral sclerosis (ALS), an age-onset neurodegenerative disease. However, clear mechanistic insights linking altered metabolism and MN death are still missing. In this study, induced pluripotent stem cells from healthy controls, familial ALS, and sporadic ALS patients were differentiated toward spinal MNs, cortical neurons, and cardiomyocytes. Metabolic flux analyses reveal an MN-specific deficiency in mitochondrial respiration in ALS. Intriguingly, all forms of familial and sporadic ALS MNs tested in our study exhibited similar defective metabolic profiles, which were attributed to hyper-acetylation of mitochondrial proteins. In the mitochondria, Sirtuin-3 (SIRT3) functions as a mitochondrial deacetylase to maintain mitochondrial function and integrity. We found that activating SIRT3 using nicotinamide or a small molecule activator reversed the defective metabolic profiles in all our ALS MNs, as well as correct a constellation of ALS-associated phenotypes.
Influence of individual biological traits on GPS fix-loss errors in wild bird tracking
Authors: García-Jiménez Ruth , Margalida Antoni , Pérez-García Juan M. 
Published: November 2020

AbstractIn recent decades, global positioning system (GPS) location data and satellite telemetry systems for data transmission have become fundamental in the study of basic ecological traits in wildlife biology. Evaluating GPS location errors is essential in assessing detailed information about the behaviour of an animal species such as migration, habitat selection, species distribution or foraging strategy. While many studies of the influence of environmental and technical factors on the fix errors of solar-powered GPS transmitters have been published, few studies have focussed on the performance of GPS systems in relation to a species’ biological traits. Here, we evaluate the possible effects of the biological traits of a large raptor on the frequency of lost fixes—the fix-loss rate (FLR). We analysed 95,686 records obtained from 20 Bearded VulturesGypaetus barbatustracked with 17 solar-powered satellite transmitters in the Pyrenees (Spain, France and Andorra), between 2006 and 2019 to evaluate the influence of biological, technical, and environmental factors on the fix-loss rate of transmitters. We show that combined effects of technical factors and the biological traits of birds explained 23% of the deviance observed. As expected, the transmitter usage time significantly increased errors in the fix-loss rate, although the flight activity of birds revealed an unexpected trade-off: the greater the proportion of fixes recorded from perched birds, the lower the FLR. This finding seems related with the fact that territorial and breeding birds spend significantly more time flying than non-territorial individuals. The fix success rate is apparently due to the interactions between a complex of factors. Non-territorial adults and subadults, males, and breeding individuals showed a significantly lower FLR than juveniles-immatures females, territorial birds or non-breeding individuals. Animal telemetry tracking studies should include error analyses before reaching any ecological conclusions or hypotheses about spatial distribution.
Mantle upwelling beneath the Apennines identified by receiver function imaging
Authors: Chiarabba Claudio , Bianchi Irene , De Gori Pasquale , Agostinetti Nicola Piana 
Published: November 2020

AbstractMagmatism, uplift and extension diffusely take place along collisional belts. Even though links between mantle dynamics and shallow deformation are becoming more evident, there is still poor understanding of how deep and surface processes are connected. In this work, we present new observations on the structure of the uppermost mantle beneath the Apennines belt. Receiver functions and seismic tomography consistently define a broad zone in the shallow mantle beneath the mountain belt where the shear wave velocities are lower than about 5% and the Vp/Vs ratio is higher than 3% than the reference values for these depths. We interpret these anomalies as a pronounced mantle upwelling with accumulation of melts at the crust-mantle interface, on top of which extensional seismicity responds to the crustal bending. The melted region extends from the Tyrrhenian side to the central part of the belt, with upraise of fluids within the crust favored by the current extension concentrated in the Apennines mountain range. More in general, mantle upwelling, following detachment of continental lithosphere, is a likely cause for elevated topography, magmatism and extension in post-collisional belts.
Construction of an artificial system for ambrein biosynthesis and investigation of some biological activities of ambrein
Authors: Yamabe Yota , Kawagoe Yukina , Okuno Kotone , Inoue Mao , Chikaoka Kanako , Ueda Daijiro , Tajima Yuko , Yamada Tadasu K. , Kakihara Yoshito , Hara Takashi , Sato Tsutomu 
Published: November 2020

AbstractAmbergris, a sperm whale metabolite, has long been used as a fragrance and traditional medication, but it is now rarely available. The odor components of ambergris result from the photooxidative degradation of the major component, ambrein. The pharmacological activities of ambergris have also been attributed to ambrein. However, efficient production of ambrein and odor compounds has not been achieved. Here, we constructed a system for the synthesis of ambrein and odor components. First, we created a new triterpene synthase, “ambrein synthase,” for mass production of ambrein by redesigning a bacterial enzyme. The ambrein yields were approximately 20 times greater than those reported previously. Next, an efficient photooxidative conversion system from ambrein to a range of volatiles of ambergris was established. The yield of volatiles was 8–15%. Finally, two biological activities, promotion of osteoclast differentiation and prevention of amyloid β-induced apoptosis, were discovered using the synthesized ambrein.
Effects of two measures of riparian plant biodiversity on litter decomposition and associated processes in stream microcosms
Authors: López-Rojo Naiara , Pérez Javier , Basaguren Ana , Pozo Jesús , Rubio-Ríos Juan , Casas J. Jesús , Boyero Luz 
Published: November 2020

AbstractPlant litter decomposition is a key ecosystem process that can be altered by global changes such as biodiversity loss. These effects can be particularly important in detritus-based ecosystems, such as headwater streams, which are mainly fuelled by allochthonous plant litter inputs. However, experiments examining effects of plant diversity on litter decomposition in streams have not reached consensus about which measures of biodiversity are more relevant. We explored the influence of two of these measures, plant species richness (SR; monocultures vs. 3-species mixtures) and phylogenetic distance (PD; species belonging to the same family vs. different families), on leaf litter decomposition and associated processes and variables (nutrient dynamics, fungal biomass and detritivore growth), in a stream microcosm experiment using litter from 9 tree species belonging to 3 families. We found a negative effect of SR on decomposition (which contradicted the results of previous experiments) but a positive effect on fungal biomass. While PD did not affect decomposition, both SR and PD altered nutrient dynamics: there was greater litter and detritivore N loss in low-PD mixtures, and greater litter P loss and detritivore P gain in monocultures. This suggested that the number of species in mixtures and the similarity of their traits both modulated nutrient availability and utilization by detritivores. Moreover, the greater fungal biomass with higher SR could imply positive effects on detritivores in the longer term. Our results provide new insights of the functional repercussions of biodiversity loss by going beyond the often-explored relationship between SR and decomposition, and reveal an influence of plant species phylogenetic relatedness on nutrient cycling that merits further investigation.
A non-field analytical method for solving problems in aero-acoustics
Authors: Kulish Vladimir , Nožička Jiří , Suchý Jakub 
Published: November 2020

AbstractIn 2000, a non-field analytical method for solving various problems of energy and information transport has been developed by Kulish and Lage. Based on the Laplace transform technique, this elegant method yields closed-form solutions written in the form of integral equations, which relate local values of an intensive properties such as, for instance, velocity, mass concentration, temperature with the corresponding derivative, that is, shear stress, mass flux, temperature gradient. Over the past 20 years, applied to solving numerous problems of energy and information transport, the method—now known as the method of Kulish—proved to be very efficient. In this paper—for the first time—the method is applied to problems in aeroacoustic. As a result, an integral relation between the local values of the acoustic pressure and the corresponding velocity perturbation has been derived. The said relation is valid for axisymmetric cases of planar, cylindrical and spherical geometries.
Aggregation induced emission behavior in oleylamine acetone system and its application to get improved photocurrent from In2S3 quantum dots
Authors: Ramya Subramaniam , Nataraj Devaraj , Krishnan Sangameswaran , Premkumar Sellan , Thrupthika Thankappan , Sangeetha Arumugam , Senthilkumar Kittusamy , Thangadurai T. Daniel 
Published: November 2020

AbstractBlue emission giving nanoscale molecular clusters of Oleylamine–Acetone system was formed by an aging assisted hydrogen bond formation between the interacting molecular systems, at room temperature. The as-formed nanoscale molecular clusters were found to be self-assembled into flower-like aggregates and shifted the emission wavelength to red colour depicting an exciton delocalization in the aggregate system. Interestingly aging process has also produced imine type binding between Oleylamine and Acetone due to the condensation reaction. The experimental conditions and formation mechanism of hydrogen bond assisted Oleylamine–Acetone molecular aggregates and imine bond assisted Oleylamine–Acetone is elaborated in this paper in a systematic experimental approach with suitable theory. Finally we have introduced this Acetone assisted aging process in In2S3 QD system prepared with Oleylamine as functional molecules. It was found that the aging process has detached Oleylamine from QD surface and as a consequence In2S3 QD embedded Oleylamine–Acetone aggregates was obtained. When this In2S3 QD embedded molecular cluster system was used as an active layer in a photo conductor device then a maximum photo current value of the order of milli Ampere was obtained. The surfactant molecules normally inhibit the charge transport between QD systems and as a result it is always problematic to have the functional molecules in the QD based transport devices. Our approach has a solution to this problem and the present paper discusses the outcome of the results in detail.
Phosphorylation of NMDA receptors by cyclin B/CDK1 modulates calcium dynamics and mitosis
Authors: Rosendo-Pineda Margarita Jacaranda , Vicente Juan Jesus , Vivas Oscar , Pacheco Jonathan , Loza-Huerta Arlet , Sampieri Alicia , Wordeman Linda , Moreno Claudia , Vaca Luis 
Published: November 2020

AbstractN-methyl-D-aspartate receptors (NMDAR) are glutamate-gated calcium channels named after their artificial agonist. NMDAR are implicated in cell proliferation under normal and pathophysiological conditions. However, the role of NMDAR during mitosis has not yet been explored in individual cells. We found that neurotransmitter-evoked calcium entry via endogenous NMDAR in cortical astrocytes was transient during mitosis. The same occurred in HEK293 cells transfected with the NR1/NR2A subunits of NMDAR. This transient calcium entry during mitosis was due to phosphorylation of the first intracellular loop of NMDAR (S584 of NR1 and S580 of NR2A) by cyclin B/CDK1. Expression of phosphomimetic mutants resulted in transient calcium influx and enhanced NMDAR inactivation independent of the cell cycle phase. Phosphomimetic mutants increased entry of calcium in interphase and generated several alterations during mitosis: increased mitotic index, increased number of cells with lagging chromosomes and fragmentation of pericentriolar material. In summary, by controlling cytosolic calcium, NMDAR modulate mitosis and probably cell differentiation/proliferation. Our results suggest that phosphorylation of NMDAR by cyclin B/CDK1 during mitosis is required to preserve mitotic fidelity. Altering the modulation of the NMDAR by cyclin B/CDK1 may conduct to aneuploidy and cancer.
A six-gene-based signature for breast cancer radiotherapy sensitivity estimation
Authors: Chen Xing , Zheng Junjie , Zhuo Min ling , Zhang Ailong , You Zhenhui 
Published: November 2020

Abstract
Breast cancer (BRCA) represents the most common malignancy among women worldwide with high mortality. Radiotherapy is a prevalent therapeutic for BRCA that with heterogeneous effectiveness among patients. Here, we proposed to develop a gene expression-based signature for BRCA radiotherapy sensitivity estimation. Gene expression profiles of BRCA samples from the Cancer Genome Atlas (TCGA) and International Cancer Genome Consortium (ICGC) were obtained and used as training and independent testing dataset, respectively. Differential expression genes (DEGs) in BRCA samples compared with their paracancerous samples in the training set were identified by using the edgeR Bioconductor package. Univariate Cox regression analysis and LASSO Cox regression method were applied to screen optimal genes for constructing a radiotherapy sensitivity estimation signature. Nomogram combining independent prognostic factors was used to predict 1-, 3-, and 5-year OS of radiation-treated BRCA patients. Relative proportions of tumor infiltrating immune cells (TIICs) calculated by CIBERSORT and mRNA levels of key immune checkpoint receptors was adopted to explore the relation between the signature and tumor immune response. As a result, 603 DEGs were obtained in BRCA tumor samples, six of which were retained and used to construct the radiotherapy sensitivity prediction model. The signature was proved to be robust in both training and testing sets. In addition, the signature was closely related to the immune microenvironment of BRCA in the context of TIICs and immune checkpoint receptors’ mRNA levels. In conclusion, the present study obtained a radiotherapy sensitivity estimation signature for BRCA, which should shed new light in clinical and experimental research.
Dual self-regulated delivery of insulin and glucagon by a hybrid patch
Authors: Wang Zejun , Wang Jinqiang , Li Hongjun , Yu Jicheng , Chen Guojun , Kahkoska Anna R. , Wu Valerie , Zeng Yi , Wen Di , Miedema Jayson R. , Buse John B. , Gu Zhen 
Published: November 2020

Significance
The efficacy of current diabetes treatments designed to reverse insulin deficiency is often limited by the frequent occurrence of acute hypoglycemia. To this end, a hybrid glucose-responsive delivery patch is engineered to function as a synthetic artificial pancreas by delivering insulin and glucagon upon hyperglycemic and hypoglycemic conditions, respectively. A mask-mediated polymerization method is developed to accommodate the insulin and glucagon modules that share a copolymerized matrix but comprise different ratios of the key monomers. The synergistic counterregulation of the two modules is found to enhance the capability of the hybrid patch in maintaining normoglycemia during plasma glucose fluctuation. This platform strategy could facilitate the development of customized therapeutic patches for closed-loop delivery in a precise manner.
Localizing Chinese games for Southeast Asian markets
Authors: Wu Zhiwei , Chen Zhuojia 
Published: November 2020

Abstract
This article explores how Chinese games are localized for Southeast Asia (SEA) markets. Based on the synthesized
insights from practitioners and gamers, we identify gaps between localization in theory and in practice. The post-gold model is
popular with Chinese game companies that usually do not consider localizing a game until it has attained domestic success. They
tend to opt for full localization rather than “deep localization” (Bernal-Merino 2011)
because adapting visuals and game mechanics is considered “icing on the cake”. Additionally, in our data, gamers seem to prefer
foreignization over domestication, while practitioners combine both strategies to create a defamiliarizing gaming experience.
Finally, the language diversity in SEA and the lingua franca status of English call for a nuanced understanding of
locale. Hence, we suggest to differentiate three types of locales (presumed, practiced, and preferred) as a
possible analytical framework to further theorize game localization from multiple perspectives of stakeholders.
Neural machine translation and the evolution of the localisation sector
Authors: O’Brien Sharon , Rossetti Alessandra 
Published: November 2020

AbstractThe localisation sector is highly technologized and evolves rapidly. Though significant consideration has been given to third-level training in localisation for Translation Studies students, the nature of the industry is such that this topic demands regular attention. Our objective was to survey employees and executive managers to understand what impact recent technological developments, including but not limited to neural machine translation (NMT), might have on future skills and training requirements for localisation linguists. Our findings are that linguists in localisation take up a variety of roles, including transcreation, data mining, and project and vendor management. NMT is considered an important advancement, and its introduction has emphasised the need for a critical use of technology, while opening new career pathways, such as data curation and annotation. Domain specialisation is recommended for those who translate, and transferable soft skills are more essential than ever. Increased industry and interdisciplinary collaborations in training are also considered valuable.
Insights into the photocatalytic degradation of triclosan over amorphous Nb2O5 catalysts
Authors: Zhang Jie , Li Dandan , Qiu Jianping , Wen Zhengru , Luo Xiaohui , Bian Chaoqun , Chen Jian , Luo Mengfei 
Published: November 2020

Abstract
Photocatalytic degradation of triclosan is one of the most promising way to eliminate triclosan pollution in water, yet it presents profound challenges to develop efficient photocatalysts to meet the demand of application. Herein, we demonstrate a facile hydrothermal-precipitation method to prepare amorphous Nb2O5 catalysts by using niobium(V) oxalate hydrate and ammonium carbonate. A series of amorphous Nb2O5 catalysts were obtained and analyzed by detail characterizations (XRD, XPS, BET and SEM). By optimizing synthesis conditions, it was found that 180/12-Nb2O5-(1:5) catalyst exhibited the optimal photocatalytic activity for triclosan degradation. Furthermore, the pseudo-first-order kinetic equations for triclosan degradation over 180/12-Nb2O5-(1:5) catalyst were investigated under conditions with different pH value. Among them, the triclosan degradation rate over 180/12-Nb2O5-(1:5) catalyst gave the highest reaction constant k (0.5 min−1) and shortest half-period (t1/2 = 0.17 min−1) at pH of 8. The presence of ionic state triclosan in base condition should be responsible for the improved degradation rate from acid to base reaction condition. Compared with molecular triclosan, ionic state triclosan exhibits very high reaction rate with radicals due to its strong electrophilic property. The high photocatalytic activity of 180/12-Nb2O5-(1:5) catalyst should be related to the presence of amorphous structure compared with calcined 180/12-Nb2O5-(1:5) catalyst with highly crystalline structure. The existence of N species doping and nano-sheet structure in catalyst is also thought to promote the catalytic reaction.
Influences of the constrained groove pressing on microstructural, mechanical, and fracture properties of brass sheets
Authors: Shahmirzaloo A , Hosseini S M , Siahsarani A , Rahmatabadi D , Hashemi R , Faraji G 
Published: November 2020

Abstract
Constrained groove pressing (CGP) was used for the production of fine-grained brass sheets in different conditions. The process was conducted up to two cycles on brass sheets at room temperature and then half cycle at the temperature of 200 °C. Optical microscopy (OM), scanning electron microscopy (SEM), microhardness measurement, and plane stress fracture toughness was used to investigate the microstructure, mechanical properties, and fracture behavior. Microhardness measurement showed the capability of the CGP process in increasing the hardness of the refined sheets. It also showed the inhomogeneity of the hardness along the thickness of the sample after the process. The Digital Image Correlation (DIC) technique was used to investigate the elastic and plastic factors of the sheets along with the major mechanical properties of samples. The results showed a slight increase and reduction in the Young modulus and Poisson’s ratios after the process, respectively. Moreover, after two CGP cycles applying half cycle at the temperature of 200 °C did not show any significant effect on these values. The strength coefficient was as like as yield and ultimate strengths increased by increasing the number of the passes. However, processing at a higher temperature of 200 °C showed lower values for the parameters, as mentioned earlier, compared to the specimens processed at room temperature. The strain hardening index experienced a major reduction after the CGP process due to the effects of strain hardening. The anisotropy coefficient, which plays a critical factor in the severe deformation of sheets, was increased after the CGP process. However, this ratio decreased in higher passes or elevated temperatures. The highest anisotropy coefficient was obtained after the first cycle of the process. Moreover, SEM observation of the fracture surface showed shearing ductile rupture mode in the processed samples rather than ductile mode due to appearing of small and elongated dimples.
Measuring transferability issues in machine-learning force fields: the example of gold–iron interactions with linearized potentials
Authors: Benoit Magali , Amodeo Jonathan , Combettes Ségolène , Khaled Ibrahim , Roux Aurélien , Lam Julien 
Published: November 2020

Abstract
Machine-learning force fields have been increasingly employed in order to extend the possibility of current first-principles calculations. However, the transferability of the obtained potential cannot always be guaranteed in situations that are outside the original database. To study such limitation, we examined the very difficult case of the interactions in gold–iron nanoparticles. For the machine-learning potential, we employed a linearized formulation that is parameterized using a penalizing regression scheme which allows us to control the complexity of the obtained potential. We showed that while having a more complex potential allows for a better agreement with the training database, it can also lead to overfitting issues and a lower accuracy in untrained systems.