bluuebunny/crossref_metadata_2025_split huggingface dataset:
4-Phenylbutyrate ameliorates apoptotic neural cell death in Down syndrome by reducing protein aggregates
Authors: Hirata Katsuya , Nambara Toshihiko , Kawatani Keiji , Nawa Nobutoshi , Yoshimatsu Hidetaka , Kusakabe Haruna , Banno Kimihiko , Nishimura Ken , Ohtaka Manami , Nakanishi Mahito , Taniguchi Hidetoshi , Arahori Hitomi , Wada Kazuko , Ozono Keiichi , Kitabatake Yasuji 
Published: August 2020

AbstractIndividuals with Down syndrome (DS) commonly show unique pathological phenotypes throughout their life span. Besides the specific effects of dosage-sensitive genes on chromosome 21, recent studies have demonstrated that the gain of a chromosome exerts an adverse impact on cell physiology, regardless of the karyotype. Although dysregulated transcription and perturbed protein homeostasis are observed in common in human fibroblasts with trisomy 21, 18, and 13, whether and how this aneuploidy-associated stress acts on other cell lineages and affects the pathophysiology are unknown. Here, we investigated cellular stress responses in human trisomy 21 and 13 neurons differentiated from patient-derived induced pluripotent stem cells. Neurons of both trisomies showed increased vulnerability to apoptotic cell death, accompanied by dysregulated protein homeostasis and upregulation of the endoplasmic reticulum stress pathway. In addition, misfolded protein aggregates, comprising various types of neurodegenerative disease-related proteins, were abnormally accumulated in trisomic neurons. Intriguingly, treatment with sodium 4-phenylbutyrate, a chemical chaperone, successfully decreased the formation of protein aggregates and prevented the progression of cell apoptosis in trisomic neurons. These results suggest that aneuploidy-associated stress might be a therapeutic target for the neurodegenerative phenotypes in DS.
The Southern Polar Front as a key to mesoplankton migratory behavior
Authors: Vedenin Andrey , Kulagin Dmitry , Musaeva Eteri , Vereshchaka Alexander 
Published: August 2020

AbstractDiel and seasonal vertical migrations of zooplankton represent a widespread phenomenon occurring in marine and freshwater environments. Diel migrations are panoceanic, while seasonal migrations usually occur in temperate and polar areas. This paper describes differences in the diel and seasonal vertical migrations in the Drake Passage north and south of the Polar Front (PF). We analyzed material of 85 stations collected in spring of 2008 and 2010 (October–November) and in summer of 2010 and 2011 (January) within the 0–300 m depth range during various time of a day. At each station we sampled the upper mixed (UL), the middle (ML), and the deeper layers (DL) bounded by hydrological gradients. Diel migrations were significantly different south and north of the PF in terms of total abundance, biomass, diversity and individual taxa density. In both seasons, mesoplankton dielly migrated between the ML/DL and the UL north of the PF and between layers below 300 m and the DL and ML south of the PF. Deeper range of diel migrations south of the PF was coupled with a general mesoplankton descent in summer period compared to spring. Conversely, north of the PF, mesoplankton ascended to upper layers in summer, which was mirrored in lesser depths of diel migrations. The differences in the plankton distribution on both sides of the PF are likely associated with variations of vertical distribution of phytoplankton. Some abundant taxa such as Aetideus sp. and Oithona plumifera showed both common (nighttime ascend) and inverted (nighttime descend) vertical migrations depending on season and position related to the PF.
Rapid and quantitative antimalarial drug efficacy testing via the magneto-optical detection of hemozoin
Authors: Molnár Petra , Orbán Ágnes , Izrael Richard , Babai Réka , Marton Lívia , Butykai Ádám , Karl Stephan , Vértessy Beáta G. , Kézsmárki István 
Published: August 2020

AbstractEmergence of resistant Plasmodium species makes drug efficacy testing a crucial part of malaria control. Here we describe a novel assay for sensitive, fast and simple drug screening via the magneto-optical detection of hemozoin, a natural biomarker formed during the hemoglobin metabolism of Plasmodium species. By quantifying hemozoin production over the intraerythrocytic cycle, we reveal that hemozoin formation is already initiated by ~ 6–12 h old ring-stage parasites. We demonstrate that the new assay is capable of drug efficacy testing with incubation times as short as 6–10 h, using synchronized P. falciparum 3D7 cultures incubated with chloroquine, piperaquine and dihydroartemisinin. The determined 50% inhibitory concentrations agree well with values established by standard assays requiring significantly longer testing time. Accordingly, we conclude that magneto-optical hemozoin detection provides a practical approach for the quick assessment of drug effect with short incubation times, which may also facilitate stage-specific assessment of drug inhibitory effects.
Urban food subsidies reduce natural food limitations and reproductive costs for a wetland bird
Authors: Evans Betsy A. , Gawlik Dale E. 
Published: August 2020

AbstractThere is a strong conservation need to understand traits of species that adapt to urban environments, but results have been equivocal. Wetland birds exhibit a strong phylogenetic signal towards urban tolerance; however, they have largely been ignored in urban studies. In their historic ranges, wetland birds inhabit dynamic systems, traveling long distances to locate food. This ability to exploit dynamic resources may translate to success in urban environments, areas characterized by novel food opportunities. We used the Wood Stork (Mycteria americana), a species of conservation concern, to determine if the ability to exploit resources in natural environments translated to exploitation of urban resources. During optimal natural foraging conditions, storks nesting in both urban and natural wetlands had narrow diet breadths and high productivity. However, during suboptimal conditions, urban stork diet expanded to include anthropogenic items, leading to increased productivity. Our study provides a mechanistic understanding of how a wetland species persists, and even thrives, in urban environments. We demonstrated that species inhabiting dynamic systems can exploit urban areas resulting in increased reproductive performance during suboptimal conditions. Together, urban environments may support biodiversity in a variety of ways, but species-specific mechanistic understanding will help highlight how to best mitigate potential threats of urbanization.
Dry eye in rheumatoid arthritis patients under TNF-inhibitors: conjunctival goblet cell as an early ocular biomarker
Authors: Usuba Fany Solange , de Medeiros-Ribeiro Ana Cristina , Novaes Priscila , Aikawa Nadia Emi , Bonfiglioli Karina , Santo Ruth Miyuki , Bonfá Eloisa , Alves Milton Ruiz 
Published: August 2020

AbstractDry eye disease (DED) is common in Rheumatoid Arthritis (RA) patients. The application of conjunctival goblet cell count as a clinical biomarker to diagnose and respond to treatment can take place in rheumatoid arthritis patients under TNF-inhibitors (TNFi) therapy. This study aimed to investigate the ocular surface parameters and the long-term effects of TNFi therapy on ocular surface features and goblet cell count of rheumatoid arthritis patients. At baseline, rheumatoid arthritis patients eligible to TNFi were compared to healthy controls (similar age/gender), regarding Ocular Surface Disease Index (OSDI) questionnaire, Schirmer I test, tear break-up time test, vital dye staining of the ocular surface, and conjunctival impression cytology. DED severity grade, impression cytology score, and goblet cell count were analyzed. Rheumatoid arthritis patients were followed after three (3 M) and 12 months (12 M), during TNFi treatment. Sixteen rheumatoid arthritis patients and 24 controls were compared: a higher frequency of abnormal OSDI (68.8% vs. 16.7%, p = 0.002), Schirmer’s test < 10 mm (37.5% vs. 8.3%, p = 0.042), meibomian gland dysfunction (50% vs. 8.3%, p = 0.007), abnormal impression cytology (75% vs. 8.3%, p < 0.001), and mild to moderate DED (81.3% vs. 4.2%, p < 0.001) were observed in rheumatoid arthritis patients, who also had lower goblet cell count [325 (274–707) cells/mm2 vs. 742 (562–863) cells/mm2, p = 0.004]. The presence of Meibomian gland dysfunction was associated with higher disease activity scores (p < 0.05). The prospective early observation of these patients at 3 M showed an increase improvement in tear production by Schirmer’s test [13 (7.5–17.5) vs. 23.5 (16–35); p = 0.001], and an improvement in impression cytology score [1 (0.5–2) vs. 1 (0–1), p = 0.031] and in goblet cell count [325 (274–707) vs. 931 (656–1,244), p < 0.001]. Eight RA responders to TNFi were also re-evaluated at 12 M with further improvement in goblet cell count [393 (275–827) vs. 872 (502–1,185) vs. 1,079 (867–1,244), p = 0.047]. Multifactorial DED is frequent in RA patients, comprising aqueous, lipid, and mucin components. TNFi prompt improves tear production and recovers the goblet cells, which can be a biomarker of the pathological process and response to therapy in this population.
A population-based epidemiology of Wilson’s disease in South Korea between 2010 and 2016
Authors: Choe Eun Ju , Choi Jong Won , Kang Minjin , Lee Yong Kang , Jeon Han Ho , Park Byung Kyu , Won Sun Young , Cho Yong Suk , Seo Jeong Hun , Lee Chun Kyon , Chung Jae Bock 
Published: August 2020

AbstractVery few population-based studies have examined the epidemiology of Wilson’s disease (WD). We investigated the epidemiology of WD using the National Health Insurance Service (NHIS) database in South Korea. We analyzed not only the statistical variables of WD, but also those of WD-related diseases. WD patients were identified with the relevant International Classification of Diseases-10 code out of 50.5 million people. We used the NHIS database from 2009 to 2016 and analyzed the incidence rate, prevalence, and clinical symptoms of WD. A total of 1,333 patients were identified. The average annual incidence rate was 3.8 per million person-years. The prevalence was 38.7 per million people. The mean diagnostic age was 26.1 ± 17.2 with earlier diagnosis in men (P = 0.0003). Among the patients, 988 (74.1%) had hepatic symptoms, 510 (38.3%) had neurologic symptoms, and 601 (45.1%) had psychiatric symptoms. Before the diagnosis of WD, 350 (26.3%) had neurologic symptoms, and 427 (32%) had psychiatric symptoms. The annual mortality rate was 0.7%. Age, liver cirrhosis, and liver failure correlated with a fatal prognosis (P < 0.05). Many patients showed neurologic and psychiatric symptoms before they were diagnosed with WD. Prognosis correlated with age, liver cirrhosis, and liver failure.
High-performance vector bending and orientation distinguishing curvature sensor based on asymmetric coupled multi-core fibre
Authors: Arrizabalaga Oskar , Sun Qi , Beresna Martynas , Lee Timothy , Zubia Joseba , Velasco Pascual Javier , Sáez de Ocáriz Idurre , Schülzgen Axel , Antonio-Lopez Jose Enrique , Amezcua-Correa Rodrigo , Villatoro Joel , Brambilla Gilberto 
Published: August 2020

AbstractFibre optic technology is rapidly evolving, driven mainly by telecommunication and sensing applications. Excellent reliability of the manufacturing processes and low cost have drawn ever increasing attention to fibre-based sensors, e.g. for studying mechanical response/limitations of aerospace composite structures. Here, a vector bending and orientation distinguishing curvature sensor, based on asymmetric coupled multi-core fibre, is proposed and experimentally demonstrated. By optimising the mode coupling effect of a seven core multi-core fibre, we have achieved a sensitivity of − 1.4 nm/° as a vector bending sensor and − 17.5 nm/m−1 as a curvature sensor. These are the highest sensitivities reported so far, to the best of our knowledge. In addition, our sensor offers several advantages such as repeatability of fabrication, wide operating range and small size and weight which benefit its sensing applications.
Twigs-derived activated carbons via H3PO4/ZnCl2 composite activation for methylene blue and congo red dyes removal
Authors: Zubir Muhammad Hadzirun Muhamad , Zaini Muhammad Abbas Ahmad 
Published: August 2020

AbstractThis work is aimed at evaluating the conversion of Pterocarpus indicus twigs into activated carbon via composite chemical activation for methylene blue and congo red dyes adsorption. The activated carbons were prepared by single-step chemical activation using zinc chloride and/or phosphoric acid at different mass impregnation ratios at 600 °C for 90 min. The activated carbons were characterized for textural properties and surface chemistry. The batch adsorption was investigated at different concentrations (5–1,000 mg/L), contact times (2–540 min) and temperatures (30–60 °C). Phosphoric acid-activated twigs carbon showed a high surface area of 1,445 m2/g with maximum methylene blue adsorption capacity of 438 mg/g. On the other hand, a composite-activated carbon yields a 217 mg/g of congo red removal. The adsorption data for both dyes fitted well with Langmuir and pseudo-second-order kinetics models, indicating the predominance of chemical adsorption through monolayer coverage of dye molecules on the homogenous surface of activated carbon. The thermodynamics properties of dye adsorption onto twigs-derived activated carbons indicated that the process is endothermic, spontaneous and favourable at high temperature. Overall, activated carbons derived from Pterocarpus indicus twigs could be effectively used for dye wastewater treatment.
A prediction model of outcome of SARS-CoV-2 pneumonia based on laboratory findings
Authors: Wu Gang , Zhou Shuchang , Wang Yujin , Lv Wenzhi , Wang Shili , Wang Ting , Li Xiaoming 
Published: August 2020

AbstractThe severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has resulted in thousands of deaths in the world. Information about prediction model of prognosis of SARS-CoV-2 infection is scarce. We used machine learning for processing laboratory findings of 110 patients with SARS-CoV-2 pneumonia (including 51 non-survivors and 59 discharged patients). The maximum relevance minimum redundancy (mRMR) algorithm and the least absolute shrinkage and selection operator logistic regression model were used for selection of laboratory features. Seven laboratory features selected in the model were: prothrombin activity, urea, white blood cell, interleukin-2 receptor, indirect bilirubin, myoglobin, and fibrinogen degradation products. The signature constructed using the seven features had 98% [93%, 100%] sensitivity and 91% [84%, 99%] specificity in predicting outcome of SARS-CoV-2 pneumonia. Thus it is feasible to establish an accurate prediction model of outcome of SARS-CoV-2 pneumonia based on laboratory findings.
Deep learning for automated sleep staging using instantaneous heart rate
Authors: Sridhar Niranjan , Shoeb Ali , Stephens Philip , Kharbouch Alaa , Shimol David Ben , Burkart Joshua , Ghoreyshi Atiyeh , Myers Lance 
Published: August 2020

AbstractClinical sleep evaluations currently require multimodal data collection and manual review by human experts, making them expensive and unsuitable for longer term studies. Sleep staging using cardiac rhythm is an active area of research because it can be measured much more easily using a wide variety of both medical and consumer-grade devices. In this study, we applied deep learning methods to create an algorithm for automated sleep stage scoring using the instantaneous heart rate (IHR) time series extracted from the electrocardiogram (ECG). We trained and validated an algorithm on over 10,000 nights of data from the Sleep Heart Health Study (SHHS) and Multi-Ethnic Study of Atherosclerosis (MESA). The algorithm has an overall performance of 0.77 accuracy and 0.66 kappa against the reference stages on a held-out portion of the SHHS dataset for classifying every 30 s of sleep into four classes: wake, light sleep, deep sleep, and rapid eye movement (REM). Moreover, we demonstrate that the algorithm generalizes well to an independent dataset of 993 subjects labeled by American Academy of Sleep Medicine (AASM) licensed clinical staff at Massachusetts General Hospital that was not used for training or validation. Finally, we demonstrate that the stages predicted by our algorithm can reproduce previous clinical studies correlating sleep stages with comorbidities such as sleep apnea and hypertension as well as demographics such as age and gender.
Thickness of the cerebral cortex shows positive association with blood levels of triacylglycerols carrying 18-carbon fatty acids
Authors: Sliz Eeva , Shin Jean , Syme Catriona , Black Sandra , Seshadri Sudha , Paus Tomas , Pausova Zdenka 
Published: August 2020

AbstractPerturbations in fatty acid (FA) metabolism as well as thinning of the cerebral cortex have been associated with cognitive decline in the elderly. Predominant FAs in the brain are docosahexaenoic acid (DHA) and arachidonic acid (ARA). Approximately 2–8% of esterified DHA and 3–5% of esterified ARA in the brain are replaced daily. DHA and ARA are derivatives of 18-carbon essential FAs, α-linolenic acid and linoleic acid, that must be imported into the brain from the circulation. In blood, FAs are primarily transported in triacylglycerols (TAGs) from which they can be released at the blood–brain-barrier and transported inside the brain. We show that circulating levels of TAGs carrying 18-carbon FAs are positively associated with cortical thickness in middle-aged adults. These associations are stronger in cortical regions with higher expression of genes regulating long-chain FA metabolism and cellular membranes, and cortical thickness in the same regions may be related to cognitive performance.
Diabetic atherosclerosis: is there a role for the hypoxia-inducible factors?
Authors: Pirri Daniela , Fragiadaki Maria , Evans Paul C. 
Published: August 2020

Abstract
Atherosclerosis is a major cause of mortality worldwide and is driven by multiple risk factors, including diabetes. Diabetes is associated with either an insulin deficiency in its juvenile form or with insulin resistance and obesity in Type 2 diabetes mellitus, and the latter is clustered with other comorbidities to define the metabolic syndrome. Diabetes and metabolic syndrome are complex pathologies and are associated with cardiovascular risk via vascular inflammation and other mechanisms. Several transcription factors are activated upon diabetes-driven endothelial dysfunction and drive the progression of atherosclerosis. In particular, the hypoxia-inducible factor (HIF) transcription factor family is a master regulator of endothelial biology and is raising interest in the field of atherosclerosis. In this review, we will present an overview of studies contributing to the understanding of diabetes-driven atherosclerosis, integrating the role of HIF in this disease with the knowledge of its functions in metabolic syndrome and diabetic scenario.
Epigenetic modification and therapeutic targets of diabetes mellitus
Authors: Singh Rajveer , Chandel Shivani , Dey Dhritiman , Ghosh Arijit , Roy Syamal , Ravichandiran Velayutham , Ghosh Dipanjan 
Published: August 2020

Abstract
The prevalence of diabetes and its related complications are increasing significantly globally. Collected evidence suggested that several genetic and environmental factors contribute to diabetes mellitus. Associated complications such as retinopathy, neuropathy, nephropathy and other cardiovascular complications are a direct result of diabetes. Epigenetic factors include deoxyribonucleic acid (DNA) methylation and histone post-translational modifications. These factors are directly related with pathological factors such as oxidative stress, generation of inflammatory mediators and hyperglycemia. These result in altered gene expression and targets cells in the pathology of diabetes mellitus without specific changes in a DNA sequence. Environmental factors and malnutrition are equally responsible for epigenetic states. Accumulated evidence suggested that environmental stimuli alter the gene expression that result in epigenetic changes in chromatin. Recent studies proposed that epigenetics may include the occurrence of ‘metabolic memory’ found in animal studies. Further study into epigenetic mechanism might give us new vision into the pathogenesis of diabetes mellitus and related complication thus leading to the discovery of new therapeutic targets. In this review, we discuss the possible epigenetic changes and mechanism that happen in diabetes mellitus type 1 and type 2 separately. We highlight the important epigenetic and non-epigenetic therapeutic targets involved in the management of diabetes and associated complications.
Disk Evolution Study Through Imaging of Nearby Young Stars (DESTINYS): A close low-mass companion to ET Cha
Authors: Ginski C. , Ménard F. , Rab Ch. , Mamajek E. E. , van Holstein R. G. , Benisty M. , Manara C. F. , Asensio Torres R. , Bohn A. , Birnstiel T. , Delorme P. , Facchini S. , Garufi A. , Gratton R. , Hogerheijde M. , Huang J. , Kenworthy M. , Langlois M. , Pinilla P. , Pinte C. , Ribas Á. , Rosotti G. , Schmidt T. O. B. , van den Ancker M. , Wahhaj Z. , Waters L. B. F. M. , Williams J. , Zurlo A. 
Published: August 2020

Context.To understand the formation of planetary systems, it is important to understand the initial conditions of planet formation, that is, the young gas-rich planet forming disks. Spatially resolved, high-contrast observations are of particular interest since substructures in disks that are linked to planet formation can be detected. In addition, we have the opportunity to reveal close companions or even planets in formation that are embedded in the disk.Aims.In this study, we present the first results of the Disk Evolution Study Through Imaging of Nearby Young Stars (DESTINYS), an ESO/SPHERE large program that is aimed at studying disk evolution in scattered light, mainly focusing on a sample of low-mass stars (< 1M⊙) in nearby (∼200 pc) star-forming regions. In this particular study, we present observations of the ET Cha (RECX 15) system, a nearby “old” classical T Tauri star (5−8 Myr, ∼100 pc), which is still strongly accreting.Methods.We used SPHERE/IRDIS in theH-band polarimetric imaging mode to obtain high spatial resolution and high-contrast images of the ET Cha system to search for scattered light from the circumstellar disk as well as thermal emission from close companions. We additionally employed VLT/NACO total intensity archival data of the system taken in 2003.Results.Here, we report the discovery, using SPHERE/IRDIS, of a low-mass (sub)stellar companion to theηCha cluster member ET Cha. We estimate the mass of this new companion based on photometry. Depending on the system age, it is either a 5 Myr, 50MJupbrown dwarf or an 8 Myr, 0.10M⊙M-type, pre-main-sequence star. We explore possible orbital solutions and discuss the recent dynamic history of the system.Conclusions.Independent of the precise companion mass, we find that the presence of the companion likely explains the small size of the disk around ET Cha. The small separation of the binary pair indicates that the disk around the primary component is likely clearing from the outside in, which explains the high accretion rate of the system.