bluuebunny/crossref_metadata_2025_split huggingface dataset:
Exploring staff conceptions of prevention and management practices in encounters with staff-directed aggression in supported housing following education and training
Authors: Maagerø-Bangstad Erlend Rinke , Sælør Knut Tore , Lillevik Ole Greger , Ness Ottar 
Published: March 2020

Abstract
Background: Staff-directed aggression is a concern for service providers in mental healthcare, frequently affecting both the quality of services and staff wellbeing. This also applies to supported housing services for people with mental health problems. Staff themselves consider training to be an important route to improve the prevention and management of staff-directed aggression. The aims of this study are to explore how staff in community supported housing conceptualize practice in prevention and management of aggression and how these conceptions develop following a local education and training endeavor in disempowerment-sensitive, de-escalating and knowledge-based risk assessment and management. Method: Phenomenography, a qualitative research approach, was adopted to pursue the study aims. The data consisted of two interviews with 13 participants from five different municipal housing facilities in Oslo, Norway. Results: The analysis led to the development of six qualitatively different, yet logically interrelated, categories of description regarding practice in encounters with staff-directed aggression: 1) Observation, alertness and awareness , 2) Established understanding and knowledge of service users , 3) Team-based risk management and deliberation , 4) Adaption of own dispositions and behaviors , 5) Reflexivity, sensitivity and care and 6) Involvement and dialogue . These conceptions were found to vary in meaning and focus; they ranged from implementing safeguarding and protective measures, to drawing on what was portrayed in terms of staff’s expert knowledge, to increasingly allowing for, and committing to, tenant perspectives in designing practice. The results indicate a moderate, yet beneficial, effect of the course on conceptual change in the participants. Conclusion: This study shows that practice in encounters with staff-directed aggression is conceptualized as complex and multifaceted by staff in community mental health services and that the various conceptions will have different consequences for the way staff-directed aggression is mitigated individually and collectively. Our findings also suggest that it is beneficial to take conceptual variation regarding practice into consideration when devising education and training to enhance staff knowledge, skills and practices.
GPs perspective on the usability of the Dutch Primary Care Practice Report: a qualitative interpretative approach
Authors: Bekker Piet De , Vink Maarten , Donker Marianne , Sterkenburg Paul , Hijden Eric van der 
Published: March 2020

Abstract
Background Audit and feedback informs healthcare providers and may affect professional practice and patient outcomes. The Primary Care Practice Report (PCPR) is a web based personalized feedback instrument for general practitioners (GPs) in the Netherlands, based on claims data. Its yearly uptake is limited. In order to improve the use and usability of the report this study aims to identify key criteria that GPs deem important for audit and feedback. Methods A qualitative interpretative approach was used. We interviewed 12 GPs about their use of the practice report. These interviews followed the Three-Step Test-Interview method. Thematic content analysis was used to investigate the perception of the GP on the report and their perspective on the usability. The interviews resulted in critical items for audit and feedback, on which all tables and graphs in the report were systematically assessed. Results From the interviews with GPs recurring criteria emerged that were identified as decisive for the effectiveness of performance feedback: content, reliability, validity and usability. The 34 tables and graphs of the PCPR were assessed using factual characteristics. Content analysis shows that the PCPR has a strong focus on costs. Assessment on validity indicates that casemix correction is always performed if relevant, but explanatory notes hardly clarify which (sub)population is measured (3/34), therefore GP’s in general have difficulty interpreting the results. Assessment on usability shows that, although benchmark figures are almost always presented based on national references, the formulation of goals or any specific attention for an action perspective is lacking because the perceived comparability with the patient group of the GP is limited. Conclusions The current PCPR does not meet key criteria for effective audit and feedback, as defined by GPs. It has a strong focus on costs instead of clinical behavior and is poorly understood when it comes to the specific population the data reflect. The results are in line with theoretical perspectives on learning and improvement of professionals. Improvement of the PCPR requires information on aspects of clinical behavior that are recognizable for GPs and they actually can influence. First steps are made to improve the method of case-mix correction in the benchmarking.
A systematic review of human giardiasis in Bangladesh: public health perspective
Authors: Hossain Biplob , Rashid Humaira , Noor Zannatun , Kabir Mamun , Miah Sohag , Siddique Abdullah , Haque Rashidul 
Published: March 2020

Abstract
Background: Giardiasis identified as a leading cause of gastrointestinal pathogen in young children worldwide, is also common in Bangladesh. There has been an emerging evidence of an association between giardiasis and child growth. This systematic review focuses only on Bangladeshi children. Prevalence of giardiasis with its adverse health effects and the advancement of diagnosis has been reviewed.Methods: A broad review of literature, electronic databases, and books within the time frame of 1970 and 2019 has been searched. Data published on giardiasis prevalence, outcomes (child growth, intestinal permeability, and diarrhea) and advances of diagnosis in Bangladesh has been searched.Results: Both assemblages A and B genotypes of Giardia lamblia are responsible for human giardiasis in Bangladesh. Recent studies on Bangladeshi children suggested Giardia species infections has an adverse impact on poor child growth which have further characterized associations with altered gut epithelial barrier dysfunction, as well as diarrhea. Indeed emerging evidence indicates the key consequence of Giardia colonization is nutrient malabsorption. Conclusions: In Bangladesh giardiasis remains a major public health concern, especially in children age <5 years but is considered as a neglected disease. In order to reduce the burden of giardiasis in developing countries such as Bangladesh, more focus is require to include this disease in public health policies.
Reliability study of measuring leg length discrepancy in patients with total hip arthroplasty using the tip of the greater trochanter
Authors: Lu Yufeng , Yuan Xuechao , Xu Peng , Hao Yangquan 
Published: March 2020

Abstract
Background Although the tip of the greater trochanter is widely used in THA to adjust leg length discrepancy (LLD), its accuracy and reliability are still unknown. The aim of this study was to investigate the reliability of measuring LLD in patients with total hip arthroplasty using the distance from the tip of the greater trochanter to the inter-teardrop line Methods 128 patients who underwent THA in our department with preoperative and postoperative bilateral hips anteroposterior(AP) radiographs were reviewed. The distance between the bilateral anterior superior iliac spine and the medial malleolus (ASIS-MM) was measured before and after the operation. The distances from the vertex of the lesser trochanter to the bi-ischial line(LT-BI), the distance from the vertex of the lesser trochanter to the inter-teardrop line(LT-IT) and the distance from the tip of greater trochanter to the inter-teardrop line(GT-IT) were measured on the bilateral hips AP radiograph preoperatively and postoperatively. Taking X-ray magnification into account when measuring data. Use one-way ANOVA and paired t test to compare the differences between the four methods. The agreements between the four methods was evaluated using Bland-Altman analysis. Results There was no statistical difference between the four methods. Comparing GT-IT with MM-ASIS, ,LT-IT and LT-BI, the preoperative P values were 0.369, 0.581, and 0.402, and the postoperative P values were 0.755, 0.502 and 0.233. Comparing LT-BI with MM-ASIS and LT-IT, the P values were 0.151 and 0.372 preoperatively, and 0.179 and 0.917 postoperatively. Comparing LT-IT with MM-ASIS, the P value was 0.924 before surgery and 0.291 after surgery. Bland-Altman indicated that there were good agreements between GT-IT and LT-IT. Conclusion It is highly reliable to evaluate the LLD of patients with THA by using the difference in the distance between the tip of the greater trochanter and the inter-teardrop line.
A novel protoapigenone analog RY10-4 induces apoptosis of breast cancer cells by regulating mitochondrial Ca2+ influx through the mitochondrial calcium uniporter
Authors: Xue Pingping , Chen Qian , Ren Xiuhua , Yang Yimin , Yang Xiaofan , Liu Dong 
Published: March 2020

Abstract
Background Protoapigenone, as a flavonoid compound with a specific nonaromatic B-ring, exhibits extraordinary antitumor activities against a broad spectrum of human cancer cells. Here we developed a novel protoapigenone analog RY10-4, which induces the apoptosis of various tumor cells, especially for breast cancer cells, but the underlying mechanism involved in the apoptotic process remains unclear. Methods MTT assay, colony-formation assay and flow cytometry were applied to evaluate the proliferation and apoptosis of breast cancer cells. Cytoplasmic calcium ([Ca2+]c) and mitochondrial calcium ([Ca2+]m) of the breast cancer cells were measured by the Fluo-2 and Rhod-2 probes, respectively. The mitochondrial reactive oxygen species (mtROS), membrane potential (ΔΨm) and permeability transition pore (mPTP) were analyzed by MitoSOX, JC-1 probes and Calcein/AM, respectively. Furthermore, Western bolt assay was adopted for the exploration of the mitochondrial apoptosis pathway. Besides, the xenograft assay was performed to investigate the role of RY10-4 in breast cancer cells in vivo. Results Obviously, RY10-4 could effectively suppress the proliferation and induce the apoptosis of breast cancer cells. Furthermore, the [Ca2+]c and [Ca2+]m of MDA-MB-231 cells were up-regulated after the treatment of RY10-4, resulting in the mtROS accumulation, ΔΨm depolarization and mPTP opening. And finally, the mitochondrial apoptosis was activated by the release of cytochrome C. Interestingly, the inhibition of mitochondrial calcium uniporter (MCU) with Ru360 attenuated the overload of [Ca2+]m and blocked the apoptosis of MDA-MB-231 cells induced by RY10-4, which was also consistent with the in vivo results. Conclusions From the results we concluded that RY10-4 could induce apoptosis of breast cancer cells by elevating [Ca2+]m through MCU. Our work contributed previously unknown insights into the mechanisms involving in the clinical efficacy of RY10-4 on breast cancer cells, which also advanced calcium homeostasis as a potential target for chemotherapeutic drugs.
14-3-3β paralog is inhibited by acetylation during differentiation to the osteogenic lineage.
Authors: López Yesica Romina Frontini , Gojanovich Aldana Daniela , Uhart Marina , Bustos Diego Martin 
Published: March 2020

Abstract
Background 14-3-3 protein family binds and regulate hundreds of serine/threonine phosphorylated proteins. Considered as redundant, ubiquitous and constantly expressed, this protein family was treated as an accessory for many signaling systems. Recently, specific biological functions were discovered for each of its seven paralogs in humans. Here, we studied the reversible inhibition by acetylation of its essential N- ε -lysine 49/51 residue during the osteogenic differentiation of human adipose-derived stem cells. Methods Human adipose-derived stem cells were obtained from healthy patients who underwent a dermolipectomy. The material was processed to obtain the ASCs from the stromal vascular fraction. Proteins of interest were analyzed by confocal microscopy and Western blot, and the expression level of selected proteins were modified by using lentivirus and specific shRNAs. Results We found that during the differentiation of ASC, the levels of 14-3-3 acK49/51 increased, showing that inhibition of 14-3-3 is necessary during this process. Among the 7 paralogs of this family, the inhibition by this posttranslational modification occurs mostly on the paralog β , located specifically in the nucleus, where 14-3-3 was described to bind to H3 histone and many transcription factors. Paralog specific inhibition by short hairpin RNA showed that silencing of 14-3-3 β gene, but not 14-3-3γ, increases significantly the osteogenic potential of the cells. Transdifferentiation from osteogenic to adipogenic linage of cell lines that were specifically silenced for 14-3-3 β o γ paralogs showed that 14-3-3b could be a negative regulator of the differentiation to the osteogenic lineage. Conclusion We showed that specifically 14-3-3 β is a negative regulator of osteogenesis. The levels of its inhibition by acetylation on lysine 51 is the key cellular mechanism to regulate it. The HBO1 acetyltransferase could be the enzyme responsible of this modification. Cells were this inhibition was emulated by decreasing the levels of expression of 14-3-3 β showed significant increased potential for the differentiation to the osteogenic lineage.
A Randomized Controlled Clinical Trial of Prolonged Stent Deployment Strategy in Primary Percutaneous Coronary Intervention for ST-segment Elevation Myocardial Infarction: A Pilot study
Authors: Ma Min , Wang Ling , Yang Zhi-gang , Diao Kai-yue , He Yong 
Published: March 2020

Abstract
Background Primary percutaneous coronary intervention (PPCI) is the most standard reperfusion strategy for ST-segment elevation myocardial infarction (STEMI), but the no-reflow phenomenon is still common and is associated with adverse outcomes. Little is known about the consequences of different strategies of deploying stents on outcomes in STEMI patients. The aim of this study was to evaluate whether prolonged inflation would decrease the no-reflow phenomenon in PPCI compared with the conventional rapid inflation/deflation strategy. Methods This was a prospective, single-center, blinded, randomized controlled trial. Patients were randomized 1:1 to a prolonged deployment strategy group or a conventional deployment strategy group. A subset of patients was included in a cardiac magnetic resonance (CMR) examination assessment. The primary outcomes were the number of patients with Thrombolysis in Myocardial Infarction (TIMI) flow grade 3, the incidence of intraoperative no-reflow/slow flow, the corrected TIMI frame count, the myocardial blush grade, and the number of patients with ST-segment resolution>50%. The secondary outcome was major cardiovascular adverse events at one month and one year following the index PPCI. Results TIMI flow grade 3 was found in 96.7% of the prolonged deployment strategy group and 63.3% of the conventional deployment group (P=0.005). The prolonged inflation strategy and inflation/deflation strategy group respectively showed the following rates: 0% vs 30% no-reflow or slow flow (P=0.002); 90% vs 66.7% ST-segment resolution ≥50% (P=0.028); 35.6±14.5 frames vs 49.18±25.2 frames on corrected TIMI frame count (P=0.014); and 60% vs 20% myocardial blush grade 3 (P=0.001). The major cardiovascular adverse event rate was 3.3% in both groups at one month and 3.3% for the prolonged inflation strategy group vs 6.7% for the rapid inflation/deflation strategy group at one year (P=1.0). In the CMR subset of cases, the presence of microvascular obstruction (MVO) was detected in 6.7% of patients in the prolonged inflation strategy group and in 50% of patients in the rapid inflation/deflation strategy group (P=0.023). Conclusions In the patients with STEMI who underwent PPCI, the effect of the prolonged inflation strategy could decease the no-reflow phenomenon and improve myocardial microcirculation perfusion, but there were no benefits for the 30-day or one-year clinical outcomes. (ClinicalTrials.gov number: NCT03199014.Registered June 23, 2017,http://https://clinicaltrials.gov/ct2/show/NCT03199014?term=NCT03199014&cntry=CN&draw=2&rank=1)
A Novel Method for the Correlation of Histological Slides and MRI Imaging of the Prostate using 3D Vertex Models
Authors: Bisson Tom , Piwonski Iris , Lohmann Sebastian , Zerbe Norman , Maxeiner Andreas , Horst David , Hufnagl Peter , Penzkofer Tobias , Elezkurtaj Sefer 
Published: March 2020

Abstract
Background: The evaluation and improvement of MRI-based grading schemes such as PI-RADS requires the correlation of histological findings with MRI images. Several approaches have been described to join the two modalities. However, these approaches have in common that they all require modification of the pathology workflow. Here, we describe a novel method to utilize vertex models for the correlation of histological findings with MRI images without affecting the histological sectioning protocol. Methods: Three patients with prostate cancer who underwent radical prostatectomy as well as presurgical MRI were selected. Prostates are segmented on the MRI images to extract a 3D vertex model using 3D Slicer. Histological sectioning is then applied on the vertex model to yield reference planes for the tissue slides. Afterwards, slides and annotated tumor regions are aligned with the reference planes using the Coherent Point Drift algorithm. Carcinomas can then be visualized in 3D using the three different techniques of block representation, convex hull and Metaballs. Results: 3D Slicer allows importing vertex models for segmentation purposes and thus the reconstructed tumors can be utilized as annotations in the original MRI images. Registration results are evaluated using the Jaccard index, which is computed by dividing the intersection area between tissue slide and reference plane by their union area. To evaluate the 3D reconstructions, the tumor-to-tissue ratio of the histological slides is computed. Block representation results in 76% of the original ratio while the convex hull causes a strong increase of 456%. The Metaball algorithm comprises a threshold parameter which affects the size of the 3D volume. Tumor-to-tissue ratios can be matched perfectly by selecting appropriate threshold values. Conclusions: Our introduced method allows the correlation of histological findings with MRI images without the need to adapt the pathologists' workflow. In fact, the accuracy of 3D reconstruction techniques needs to be further optimized, but in return the method can be applied to the entire set of archive cases.
Rates and Correlates of Involuntary Hospitalization in a Large Psychiatric Hospital 2014-2017 under China’s Mental Health Law introduced in 2013
Authors: Ma Yarong , Rosenheck Robert , He Hongbo 
Published: March 2020

Abstract
Background: Involuntary hospitalization (IH) is a controversial issue in psychiatry as it risks violation of the human right to autonomy. This study aimed to examine the rates and correlates of IH in a large psychiatric hospital from 2014-2017 after China’s Mental Health Law (MHL) was implemented in 2013 specifying criteria for IH and a structured assessment was required for IH.Methods: Unduplicated electronic medical records concerning all inpatients admitted to the Guangzhou Psychiatric Hospital with primary psychiatric diagnoses were examined and trends in annual IH rates were examined. We extracted medical, sociodemographic and socioeconomic data from these records to identify correlates of IH using bivariate chi square tests followed by logistic regression analysis. Results: Of 11,086 hospitalized patients, there was a significant but small increase from 71.6% to 74.9% in rates of IH in the years after the structured assessment was implemented. Logistic regression analysis showed IH was positively associated with being younger, having a local residence, and a diagnosis of bipolar disorder, schizophrenia spectrum disorders or a substance abuse disorder as compared to those diagnosed with major depressive disorder.Conclusions: Adjusting for these factors those with IH had a longer length of stays. Under China’s Mental Health Law in 2013, and a locally implemented checklist, the adjusted risk of IH increased significantly, but by only 3.3% from 2014 to 2017. IH was greatest younger patients with severe mental illnesses, and those with local access to psychiatric hospital services.
Full-length transcriptome sequencing of the identified fruit shape-related genes of the Olecranon honey peach
Authors: Liu Jianliang , Bao Yao , Wang Qin , Liu Huifan 
Published: March 2020

Abstract
Background: The olecranon honey peach belongs to the peach class. In terms of the fruit type, the Olecranon honey peach, shaped like an eagle’s beak, is larger than others. In this study, Full-length transcriptome sequencing of the Olecranon honey peach and the bud mutant variant of the peach was performed by Oxford Nanopore Technologies to comparatively analyze the differentially expressed genes and the transcriptome, to identify important fruit shape-related genes.Results:Full-length transcriptome sequencing was performed to analyze the two peaches. Consensus isoform was obtained and compared to the reference genome for a de-redundancy analysis. As a result, a final set of 58,596 transcript sequences was obtained. A total of 21,745 simple sequence repeats were obtained, and 18,322 alternative splicing (AS) events were identified. The comparative analysis of the non-redundant transcript revealed 2530 new gene loci and 37,364 novel transcripts. A total of 457 genes were differentially expressed in the two groups, including 169 up-regulated genes and 288 down-regulated genes. A total of 1519 transcripts were differentially expressed, of which 552 were up-regulated and 997 were down-regulated. Conclusions: In the case of the plant hormone signal transduction pathway, identified by the KEGG annotation, our analyses revealed that differential expression of genes 9229, 26004, 22504, 2822, 2826, 2824, ONT.1953, ONT.1950, and ONT.1953 was related to the shape of the peach and may regulate the production of large fruits, via endogenous hormones, secondary metabolites, and signal transduction. This study provided useful information on the shape-related genes and transcripts in the Olecranon honey peach.