bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mid age APOE ε4 carriers show memory-related functional differences and disrupted structure-function relationships in hippocampal regions
Authors: Evans Simon L. , Dowell Nicholas G. , Prowse Fenella , Tabet Naji , King Sarah L. , Rusted Jennifer M. 
Published: February 2020

AbstractCarriers of the APOE e4 allele are at higher risk of age-related cognitive decline and Alzheimer’s disease (AD). The underlying neural mechanisms are uncertain, but genotype differences in medial temporal lobe (MTL) functional activity and structure at mid-age might contribute. We tested 16 non-e4 and 16 e4 carriers (aged 45–55) on a subsequent memory task in conjunction with MRI to assess how hippocampal volume (from T1 structural) and microstructure (neurite orientation-dispersion, from NODDI) differs by genotype and in relation to memory encoding. No previous study has investigated APOE effects on hippocampal microstructure using NODDI. Recall performance did not differ by genotype. A genotype by condition interaction in left parahippocampus indicated that in e4 carriers activity did not differentiate subsequently remembered from forgotten words. Hippocampal volumes and microstructure also did not differ by genotype but hippocampal volumes correlated positively with recognition performance in non-e4 carriers only. Similarly, greater hippocampal neurite orientation-dispersion was linked to better recall but only in non-e4s. Thus, we suggest that mid-age e4 carriers show a breakdown of normal MTL activation and structure-performance relationships. This could reflect an inability to utilise compensatory mechanisms, and contribute to higher risk of cognitive decline and AD in later life.
Metaphylogenetic analysis of global sewage reveals that bacterial strains associated with human disease show less degree of geographic clustering
Authors: Ahrenfeldt Johanne , Waisi Madina , Loft Isabella C. , Clausen Philip T. L. C. , Allesøe Rosa , Szarvas Judit , Hendriksen Rene S. , Aarestrup Frank M. , Lund Ole 
Published: February 2020

AbstractKnowledge about the difference in the global distribution of pathogens and non-pathogens is limited. Here, we investigate it using a multi-sample metagenomics phylogeny approach based on short-read metagenomic sequencing of sewage from 79 sites around the world. For each metagenomic sample, bacterial template genomes were identified in a non-redundant database of whole genome sequences. Reads were mapped to the templates identified in each sample. Phylogenetic trees were constructed for each template identified in multiple samples. The countries from which the samples were taken were grouped according to different definitions of world regions. For each tree, the tendency for regional clustering was determined. Phylogenetic trees representing 95 unique bacterial templates were created covering 4 to 71 samples. Varying degrees of regional clustering could be observed. The clustering was most pronounced for environmental bacterial species and human commensals, and less for colonizing opportunistic pathogens, opportunistic pathogens and pathogens. No pattern of significant difference in clustering between any of the organism classifications and country groupings according to income were observed. Our study suggests that while the same bacterial species might be found globally, there is a geographical regional selection or barrier to spread for individual clones of environmental and human commensal bacteria, whereas this is to a lesser degree the case for strains and clones of human pathogens and opportunistic pathogens.
Influence of UV light irradiation on the corrosion behavior of electrodeposited Ni and Cu nanocrystalline foils
Authors: Deng Shu-hao , Lu Hao , Li D. Y. 
Published: February 2020

AbstractInfluence of ultraviolet (UV) light irradiation on the corrosion behavior of electrodeposited Ni and Cu nanocrystalline foils in 3.5% NaCl solution was studied by means of electrochemical methods, electron work function (EWF) analysis, and characterization with atomic force microscopy (AFM) and X-ray photoelectron spectroscopy (XPS). It was demonstrated that the influence of solar light on corrosion of the metals was non-negligible, which could be very different for different materials. The UV light irradiation resulted in an increase in corrosion resistance of the Cu foil but showed an opposite influence on that of the Ni foil. Based on surface state analysis, it was concluded that the UV irradiation altered the surface oxide films. The UV light induced the formation of Cu2O on Cu, which is more stable and compacted than naturally formed CuO film. However, the UV light accelerated the formation of Ni2O3, which is loose, porous and brittle, compared to naturally formed NiO on Ni. The changes in oxide films were responsible for the opposite variations in the corrosion behavior of the Cu and Ni nanocrystalline foils caused by the UV light irradiation.
Slc20a1/Pit1 and Slc20a2/Pit2 are essential for normal skeletal myofiber function and survival
Authors: Chande Sampada , Caballero Daniel , Ho Bryan B. , Fetene Jonathan , Serna Juan , Pesta Dominik , Nasiri Ali , Jurczak Michael , Chavkin Nicholas W. , Hernando Nati , Giachelli Cecilia M. , Wagner Carsten A. , Zeiss Caroline , Shulman Gerald I. , Bergwitz Clemens 
Published: February 2020

AbstractLow blood phosphate (Pi) reduces muscle function in hypophosphatemic disorders. Which Pi transporters are required and whether hormonal changes due to hypophosphatemia contribute to muscle function is unknown. To address these questions we generated a series of conditional knockout mice lacking one or both house-keeping Pi transporters Pit1 and Pit2 in skeletal muscle (sm), using the postnatally expressed human skeletal actin-cre. Simultaneous conditional deletion of both transporters caused skeletal muscle atrophy, resulting in death by postnatal day P13. smPit1−/−, smPit2−/− and three allele mutants are fertile and have normal body weights, suggesting a high degree of redundance for the two transporters in skeletal muscle. However, these mice show a gene-dose dependent reduction in running activity also seen in another hypophosphatemic model (Hyp mice). In contrast to Hyp mice, grip strength is preserved. Further evaluation of the mechanism shows reduced ERK1/2 activation and stimulation of AMP kinase in skeletal muscle from smPit1−/−; smPit2−/− mice consistent with energy-stress. Similarly, C2C12 myoblasts show a reduced oxygen consumption rate mediated by Pi transport-dependent and ERK1/2-dependent metabolic Pi sensing pathways. In conclusion, we here show that Pit1 and Pit2 are essential for normal myofiber function and survival, insights which may improve management of hypophosphatemic myopathy.
Intestinal Alkaline Phosphatase Exerts Anti-Inflammatory Effects Against Lipopolysaccharide by Inducing Autophagy
Authors: Singh Sudha B. , Carroll-Portillo Amanda , Coffman Cristina , Ritz Nathaniel L. , Lin Henry C. 
Published: February 2020

AbstractIntestinal alkaline phosphatase (IAP) regulates bicarbonate secretion, detoxifies lipopolysaccharide (LPS), regulates gut microbes, and dephosphorylates proinflammatory nucleotides. IAP also exhibits anti-inflammatory effects in a Toll-like Receptor-4 (TLR-4) dependent manner. However, it is not known whether IAP induces autophagy. We tested the hypothesis that IAP may induce autophagy which may mediate the anti-inflammatory effects of IAP. We found that exogenous IAP induced autophagy in intestinal epithelial cells and in macrophages. TLR4INC34 (C34), a TLR4 signaling inhibitor, suppressed IAP-induced autophagy. IAP also inhibited LPS-induced IL-1β mRNA expression and activation of NF-κB. When autophagy was blocked by 3-methyladenine (3MA) or by Atg5 siRNA, IAP failed to block LPS-mediated effects. IAP also upregulated autophagy-related gene expression in small intestine in mice. We administered either vehicle or IAP (100 U/ml) in drinking water for 14 days in C57BL/6 mice. Mice were sacrificed and ileal tissues collected. Increased expression of Atg5, Atg16, Irgm1, Tlr4, and Lyz genes was observed in the IAP treated group compared to the vehicle treated group. Increase in Atg16 protein expression and fluorescence intensity of LC3 was also observed in IAP-treated tissues compared to the vehicle-treated tissues. Thus, our study lays the framework for investigating how IAP and autophagy may act together to control inflammatory conditions.
Scaling sports equipment for children promotes functional movement variability
Authors: Buszard Tim , Garofolini Alessandro , Reid Machar , Farrow Damian , Oppici Luca , Whiteside David 
Published: February 2020

AbstractScaling sports equipment to match the physical development of children allows motor skills to be performed with greater success and with more desirable movement patterns. It is unknown, however, how scaled equipment affects movement variability – a key factor associated with coordination. Our aim was to identify whether scaled sports equipment facilitates coordination and functional movement variability in children when performing a hitting for accuracy task in tennis. Twenty-five children were asked to execute a forehand stroke with the aim of hitting the ball to a target located 10 metres away. Participants performed the task in two conditions – a scaled equipment condition and a full-sized equipment condition. Scaled equipment led to superior hitting accuracy and greater temporal stability of the swing compared to full-sized equipment. Scaled equipment also afforded the emergence of a functional coupling between upper arm and forearm movement variability which helped regulate the distance between the shoulder and the racket. Comparatively there was a lack of coupling when full-sized equipment was used. Hence, scaled equipment promoted functional movement variability, whereas full-sized equipment resulted in the freezing of mechanical degrees of freedom. This suggests that children’s skill acquisition could be hindered and potentially regress when using inappropriately sized equipment.
An electronic alert system increases screening for hepatitis B and C and improves management of patients with haematological disorders
Authors: Riveiro-Barciela Mar , Gubern Paula , Roade Luisa , Abrisqueta Pau , Carreras María José , Farriols Anna , Bosch Francesc , Esteban Rafael , Buti María 
Published: February 2020

AbstractTreatment of haematological disorders in patients with chronic hepatitis B or resolved infection (anti-HBc-positive) is associated with a risk of hepatitis B reactivation. Moreover, patients with chronic hepatitis C have a higher risk of haematological malignancies than general population. An electronic alert system was developed to promote screening of hepatitis B (HBV) and C (HCV) in patients starting haematological therapies. The system included screening and linkage to care and a request for testing in those without data. From March, 2017 to March, 2018 data from 420 consecutive patients with haematological diseases were included. At first prescription before the alerts, the HCV and HBV screening rate was 60.5%. Following the alerts, an additional 115 were screened, increasing the overall screening rate to 87.9%. Anti-HBc alone was detected in 57, anti-HCV in 13, and HBsAg in 2 patients. Overall, 68% of patients with any viral hepatitis markers were previously not know, and the impact was particularly important for anti-HBc detection (47/57 unknown). Nucleoside analogues were prescribed in 28 (49.1%) anti-HBc-positive and the 2 HBsAg-positive patients. Prospective follow-up with HBV DNA and HBsAg testing showed no cases of HBV reactivation. An estimated 1.2 HBV reactivations were avoided as consequence of the alert system. In summary, an electronic alert system increased viral hepatitis screening in patients receiving haematological treatment and led to improvements in the management of these patients, including avoided HBV reactivation.
Combined HER3-EGFR score in triple-negative breast cancer provides prognostic and predictive significance superior to individual biomarkers
Authors: Ogden Angela , Bhattarai Shristi , Sahoo Bikram , Mongan Nigel P. , Alsaleem Mansour , Green Andrew R. , Aleskandarany Mohammed , Ellis Ian O. , Pattni Sonal , Li Xiaoxian , Moreno Carlos S. , Krishnamurti Uma , Janssen Emiel A. , Jonsdottir Kristin , Rakha Emad , Rida Padmashree , Aneja Ritu 
Published: February 2020

AbstractEpidermal growth factor receptor (EGFR) and human epidermal growth factor receptor 3 (HER3) have been investigated as triple-negative breast cancer (TNBC) biomarkers. Reduced EGFR levels can be compensated by increases in HER3; thus, assaying EGFR and HER3 together may improve prognostic value. In a multi-institutional cohort of 510 TNBC patients, we analyzed the impact of HER3, EGFR, or combined HER3-EGFR protein expression in pre-treatment samples on breast cancer-specific and distant metastasis-free survival (BCSS and DMFS, respectively). A subset of 60 TNBC samples were RNA-sequenced using massive parallel sequencing. The combined HER3-EGFR score outperformed individual HER3 and EGFR scores, with high HER3-EGFR score independently predicting worse BCSS (Hazard Ratio [HR] = 2.30, p = 0.006) and DMFS (HR = 1.78, p = 0.041, respectively). TNBCs with high HER3-EGFR scores exhibited significantly suppressed ATM signaling and differential expression of a network predicted to be controlled by low TXN activity, resulting in activation of EGFR, PARP1, and caspases and inhibition of p53 and NFκB. Nuclear PARP1 protein levels were higher in HER3-EGFR-high TNBCs based on immunohistochemistry (p = 0.036). Assessing HER3 and EGFR protein expression in combination may identify which adjuvant chemotherapy-treated TNBC patients have a higher risk of treatment resistance and may benefit from a dual HER3-EGFR inhibitor and a PARP1 inhibitor.
EMG-Assisted Muscle Force Driven Finite Element Model of the Knee Joint with Fibril-Reinforced Poroelastic Cartilages and Menisci
Authors: Esrafilian A. , Stenroth L. , Mononen M. E. , Tanska P. , Avela J. , Korhonen R. K. 
Published: February 2020

AbstractAbnormal mechanical loading is essential in the onset and progression of knee osteoarthritis. Combined musculoskeletal (MS) and finite element (FE) modeling is a typical method to estimate load distribution and tissue responses in the knee joint. However, earlier combined models mostly utilize static-optimization based MS models and muscle force driven FE models typically use elastic materials for soft tissues or analyze specific time points of gait. Therefore, here we develop an electromyography-assisted muscle force driven FE model with fibril-reinforced poro(visco)elastic cartilages and menisci to analyze knee joint loading during the stance phase of gait. Moreover, since ligament pre-strains are one of the important uncertainties in joint modeling, we conducted a sensitivity analysis on the pre-strains of anterior and posterior cruciate ligaments (ACL and PCL) as well as medial and lateral collateral ligaments (MCL and LCL). The model produced kinematics and kinetics consistent with previous experimental data. Joint contact forces and contact areas were highly sensitive to ACL and PCL pre-strains, while those changed less cartilage stresses, fibril strains, and fluid pressures. The presented workflow could be used in a wide range of applications related to the aetiology of cartilage degeneration, optimization of rehabilitation exercises, and simulation of knee surgeries.
IgG-Fc glycosylation before and after rituximab treatment in immune thrombocytopenia
Authors: Schmidt David E. , de Haan Noortje , Sonneveld Myrthe E. , Porcelijn Leendert , van der Schoot C. Ellen , de Haas Masja , Zwaginga Jaap-Jan , Wuhrer Manfred , Vidarsson Gestur 
Published: February 2020

AbstractThe interactions of antibodies with myeloid Fcγ receptors and the complement system are regulated by an Asn297-linked glycan in the Fc portion of IgG. Alterations of serum IgG-Fc glycosylation have been reported in various autoimmune diseases, and correlate with treatment response and disease activity. We hypothesized that IgG-Fc glycosylation is altered in immune thrombocytopenia (ITP) and associates with response to anti-CD20 monoclonal antibody treatment (rituximab). IgG-Fc glycosylation was analyzed by liquid chromatography-mass spectrometry. We found that IgG-Fc glycosylation was identical between refractory ITP patients (HOVON64 trial; N = 108) and healthy controls (N = 120). Two months after rituximab treatment, we observed a shift in Fc glycosylation, with a mean 1.7% reduction in galactosylation for IgG1 and IgG4 and a mean 1.5% increase for bisection in IgG1, IgG2/3 and IgG4 (adjusted p < 1.7 × 10−3 and p < 2 × 10−4, respectively). Neither baseline nor longitudinal changes in IgG-Fc glycosylation after rituximab were associated with clinical treatment response. We conclude that IgG-Fc glycosylation in refractory ITP is similar to healthy controls and does not predict treatment responses to rituximab. The observed changes two months after treatment suggest that rituximab may influence total serum IgG-Fc glycosylation. Overall, our study suggests that the pathophysiology of refractory ITP may differ from other autoimmune diseases.
Antibacterial effects of low-temperature plasma generated by atmospheric-pressure plasma jet are mediated by reactive oxygen species
Authors: Nicol McKayla J. , Brubaker Timothy R. , Honish Brian J. , Simmons Alyssa N. , Kazemi Ali , Geissel Madison A. , Whalen Connor T. , Siedlecki Christopher A. , Bilén Sven G. , Knecht Sean D. , Kirimanjeswara Girish S. 
Published: February 2020

AbstractEmergence and spread of antibiotic resistance calls for development of non-chemical treatment options for bacterial infections. Plasma medicine applies low-temperature plasma (LTP) physics to address biomedical problems such as wound healing and tumor suppression. LTP has also been used for surface disinfection. However, there is still much to be learned regarding the effectiveness of LTP on bacteria in suspension in liquids, and especially on porous surfaces. We investigated the efficacy of LTP treatments against bacteria using an atmospheric-pressure plasma jet and show that LTP treatments have the ability to inhibit both gram-positive (S. aureus) and gram-negative (E. coli) bacteria on solid and porous surfaces. Additionally, both direct LTP treatment and plasma-activated media were effective against the bacteria suspended in liquid culture. Our data indicate that reactive oxygen species are the key mediators of the bactericidal effects of LTP and hydrogen peroxide is necessary but not sufficient for antibacterial effects. In addition, our data suggests that bacteria exposed to LTP do not develop resistance to further treatment with LTP. These findings suggest that this novel atmospheric-pressure plasma jet could be used as a potential alternative to antibiotic treatments in vivo.
The patterns of Non-communicable disease Multimorbidity in Iran: A Multilevel Analysis
Authors: Khorrami Zahra , Rezapour Maysam , Etemad Koorosh , Yarahmadi Shahin , Khodakarim Soheila , Mahdavi Hezaveh Alireza , Kameli Mohammadesmail , Khanjani Narges 
Published: February 2020

AbstractThe prevalence of non-communicable diseases is increasing worldwide. Multimorbidity and long-term medical conditions is common among these patients. This study aimed to investigate the patterns of non-communicable disease multimorbidity and their risk factors at the individual and aggregated level. Data was inquired from the nationwide survey performed in 2011, according to the WHO stepwise approach on NCD risk factors. A latent class analysis on multimorbidity components (11 chronic diseases) was performed and the association of some individual and aggregated risk factors (urbanization) with the latent subclasses was accessed using multilevel multinomial logistic regression. Latent class analysis revealed four distinct subclasses of multimorbidity among the Iranian population (10069 participants). Musculoskeletal diseases and asthma classes were seen in both genders. In males, the odds of membership in the diabetes class was 41% less by increasing physical activity; but with increased BMI, the odds of membership in the diabetes class was 1.90 times higher. Tobacco smoking increased the odds of membership in the musculoskeletal diseases class, 1.37 and 2.30 times for males and females, respectively. Increased BMI and low education increased the chances of females’ membership in all subclasses of multimorbidity. At the province level, with increase in urbanization, the odds of membership in the diabetes class was 1.28 times higher among males (P = 0.027). Increased age, higher BMI, tobacco smoking and low education are the most important risk factors associated with NCD multimorbidity among Iranians. Interventions and policies should be implemented to control these risk factors.
A quantitative study of stress fields ahead of a slip band blocked by a grain boundary in unalloyed magnesium
Authors: Andani Mohsen Taheri , Lakshmanan Aaditya , Karamooz-Ravari Mohammadreza , Sundararaghavan Veera , Allison John , Misra Amit 
Published: February 2020

AbstractStress localization ahead of a slip band blocked by a grain boundary is measured for three different grain boundaries in unalloyed Mg using high-resolution electron backscatter diffraction (HR-EBSD). The results are compared with a theoretical dislocation pile-up model, from which slip system resistance and micro-Hall–Petch coefficients for different grain boundary types are deduced. The results indicate that grain boundary character plays a crucial role in determining micro-Hall–Petch coefficients, which can be used to strengthen classical crystal plasticity constitutive models to make predictions linked to the effect of grain boundary strengthening.