bluuebunny/crossref_metadata_2025_split huggingface dataset:
The characteristics of mussel‐inspired nHA/OSA injectable hydrogel and repaired bone defect in rabbit
Authors: Liu Chen , Wu Juan , Gan Donglin , Li Zhiqiang , Shen Jun , Tang Pengfei , Luo Shiyu , Li Pengfei , Lu Xiong , Zheng Wei 
Published: November 2019

AbstractWith the rapid development of minimally invasive techniques in orthopedics, minimally invasive surgery combined with injection of bone repair materials has attracted increasing attention for the treatment of bone defects. Inspired by material in mussels, we decorated nanohydroxyapatite (nHA) with dopamine (DA) to form polydopamine (PDA)‐decorated nHA (PHA). Then, we introduced PHA into a Schiff base reaction of oxidized sodium alginate (OSA) and gelatin (Gel) to prepare an injectable bone repair hydrogel under mild conditions. Subsequently, the injectability, morphology, mechanical, swelling, and degradation properties of the hydrogel were studied. Then, bone marrow mesenchymal stem cells (BMSCs) were cocultured with the hydrogels to investigate the cytotoxicity, proliferation, and osteogenic differentiation properties of the hydrogel. Finally, the bone repair ability of the OSA‐Gel‐PHA hydrogels was explored using a 12 weeks rabbit bone defect model. The results of tube inversion and rheological test showed that the hydrogel gelation time was 3–7 min, which will be suitable for clinical operation. The results of SEM, compression tests, rheological tests, swelling and degradation properties tests showed that the addition of PHA changed the microstructure of the hydrogels from porous structure to layered structure, not only improved the compressive strength, toughness, elastic modulus, storage modulus of the hydrogels, but also reduced the swelling properties and degradation rate of the hydrogels, making it more conducive to clinical operations. The results of laser confocal, MTT assay, alkaline phosphatase (ALP) assay and in vivo animal experiments showed that the addition of PHA not only nontoxic, but also promoted the adhesion, proliferation and osteogenic differentiation of BMSCs, as well as the repair and maturation of bone. The application of injectable bone repair materials not only solves the problem of bone defect filling, but also avoids a series of complications caused by open surgery, providing new method for the treatment of bone defects.
Diagnostic performance for declined microRNA‐133a in pancreatic cancer
Authors: Wang Zhenyong 
Published: November 2019

AbstractMicroRNA‐133a (MiR‐133a) is proven to exhibit a decreasing tendency in several cancers, as well as pancreatic cancer. Through the present study, we inspected performance for serum miR‐133a in diagnosing pancreatic cancer. Serum samples were collected from 110 pancreatic cancer and 64 healthy persons. Relative messenger RNA level for miR‐133a in serum specimens was gauged adopting quantitative real‐time polymerase chain reaction (qRT‐PCR), and compared betwixt two groups employing the Student t test. Receiver operating characteristics (ROC) analysis evaluated miR‐133a performance in diagnosing pancreatic cancer. MiR‐133a displayed a declining trend among pancreatic cancer samples, compared to the healthy controls (P < .001). The reduced miR‐133a degree held strong relation to tumor dimension (P = .002), vessel invasion (P = .004), tumor lymph node metastasis stage (P = .002), and lymph node metastasis (P < .001). In addition, ROC analysis demonstrated that the area under the curve value was 0.893, accompanied by a sensitivity of 90.6% and a specificity of 87.2%, revealing fine execution for serum miR‐133a in diagnosing cancer. The downregulation of miR‐133a might possess a tight relation to hostile advancement in pancreatic cancer. Serum miR‐133a could function as a potential diagnostic indicator for pancreatic cancer.
Detection and clinical characteristics analysis of respiratory viruses in hospitalized children with acute respiratory tract infections by a GeXP‐based multiplex‐PCR assay
Authors: Huang Huanhuan , Chen Suqing , Zhang Xiaoyan , Hong Linliang , Zeng Yongbin , Wu Bin 
Published: November 2019

AbstractBackgroundThe information regarding viral epidemiology and clinical characteristics in hospitalized children with acute respiratory tract infection (ARTI) in central Fujian is limited. In this study, we aimed at analyzing the viral epidemiology and clinical characteristics of ARTI in hospitalized children admitted to The First Affiliated Hospital of Fujian Medical University.MethodsCohort of 386 hospitalized children (31 days to 15 years) diagnosed with ARTI admitted to the Department of Pediatrics from January 1, 2018, to December 31, 2018, was enrolled in this study. Nasopharyngeal swab or sputum samples on the day of hospitalization were tested for 11 viruses via a GeXP‐based multiplex‐PCR assay. The viral profiles and clinical characteristics were analyzed.ResultsThe overall positive rate of the samples was 43.26% (167/386). Among the 167 positive samples, 134 (80.24%, 134/167) had a single virus and 33 (19.76%, 33/167) had multiple viruses. There was a significant difference in the frequency of single vs mixed infections among positive samples (80.24% vs 19.76%; χ2 = 122.168, P = .000) as well as among the total examined samples (34.72% vs 8.55%; χ2 = 77.945, P = .000). Human rhinovirus was the most prevalent virus (17.36%, 67/386), followed by influenza A (5.96%, 23/386) and human adenovirus (5.70%, 22/386). There was no significant difference in the etiological distribution of viral pathogens between males and females (χ2 = 0.480, P = .489). Viral infections were more likely to occur in the winter‐spring months than in the summer‐autumn months (52.51% vs 33.53%, χ2 = 13.830, P = .000).ConclusionsThe GeXP‐based multiplex PCR is an accurate and high‐throughput assay allows us to quickly detect multiple respiratory viruses simultaneously in pediatric patients. Our study provides information on the viral profiles and clinical characteristics in hospitalized children with ARTI, which would help better effective prevention strategies.
GPX2 silencing relieves epithelial–mesenchymal transition, invasion, and metastasis in pancreatic cancer by downregulating Wnt pathway
Authors: Li Fuzhou , Dai Lan , Niu Jixiang 
Published: November 2019

AbstractGlutathione peroxidase 2 (GPX2) participates in many cancers including pancreatic cancer (PC), and overexpression of GPX2 promotes tumor growth. Herein, we identified the role of GPX2 in epithelial–mesenchymal transformation (EMT), invasion, and metastasis in PC. Bioinformatics prediction was applied to select PC‐related genes. The regulatory function of GPX2 in PC was explored by treatment with short hairpin RNA against GPX2 or LiCl (activator of wingless‐type MMTV integration site [Wnt] pathway) in PC cells. GPX2 level in PC tissues, the levels of GPX2, β‐catenin, Vimentin, Snail, epithelial‐cadherin (E‐cadherin), matrix metalloproteinase 2 (MMP2), MMP9, and Wnt2 in cells were determined. Subsequently, cell proliferation, invasion, and metastasis were assayed. Bioinformatics analysis revealed that GPX2 was involved in PC development mediated by the Wnt pathway. GPX2 was highly expressed in PC tissues. GPX2 silencing downregulated levels of β‐catenin, Vimentin, Snail, MMP2, MMP9, and Wnt2 but upregulated levels of E‐cadherin. It was confirmed that GPX2 silencing suppressed PC cell proliferation, metastasis, and invasion. Furthermore, the trend of EMT and invasion and metastasis of PC induced by the LiCl‐activated Wnt pathway was reversed when the GPX2 was silenced. GPX2 silencing could inhibit the Wnt pathway, subsequently suppress PC development.
Calcitriol decreases live Porphyromonas gingivalis internalized into epithelial cells and monocytes by promoting autophagy
Authors: Hu Xinyue , Niu Li , Ma Chunliang , Huang Yuehua , Yang Xue , Shi Yakun , Pan Chunling , Liu Jingbo , Wang Hongyan , Li Qian , Geng Fengxue , Tang Xiaolin 
Published: November 2019

AbstractBackgroundThe present study aimed to explore the effects of the active form of vitamin D (calcitriol, 1α,25‐dihydroxyvitamin D3, 1α,25 (OH)2D3, 1,25D) on live Porphyromonas gingivalis internalized into KB cells and U937 cells.MethodsQuantitative real‐time polymerase chain reaction method was used to evaluate the number of surviving P. gingivalis internalized into KB cells and U937 cells. Transmission electron microscopy was used to detect P. gingivalis in cells. A western blot analysis was performed to observe LC3 expressions.Results1) Treatment with 1,25D decreased the number of live P. gingivalis in KB cells and U937 cells in a dose‐dependent manner. 2) Dividing P. gingivalis were found only in KB cells but not in U937 cells. The cell walls of most P. gingivalis in KB cells were intact, while those in U937 cells were disrupted. Treatment with 1,25D promoted the encapsulation of P. gingivalis in autophagosomes in both KB and U937 cells. 3) Both 1,25D treatment and P. gingivalis infection increased the LC3 II/I ratio. Furthermore, 1,25D treatment increased the P. gingivalis‐upregulated LC3 II/I ratio. 4) Treatment with 3‐methyladenine (3‐MA) decreased the number of P. gingivalis by 11.41% in KB cells, while increased that by 121.51% in U937 cells. Under 1,25D treatment conditions, 3‐MA treatment increased the number of P. gingivalis by 88.71% in KB cells and by 284.70% in U937 cells.ConclusionsAutophagy may facilitate P. gingivalis survival in KB cells and eliminate P. gingivalis in U937 cells. Treatment with 1,25D may help decrease the number of live P. gingivalis in KB cells and U937 cells by promoting functional autophagy.
Liquiritin elicitation can increase the content of medicinally important glucosinolates and phenolic compounds in Chinese kale plants
Authors: Akram Waheed , Saeed Taiba , Ahmad Aqeel , Yasin Nasim A , Akbar Muhammad , Khan Waheed U , Ahmed Shakeel , Guo Juxian , Luo Wenlong , Wu Tingquan , Li Guihua 
Published: November 2019

AbstractBACKGROUNDBrassica oleracea var. alboglabra (Chinese kale) is an important vegetable grown in southern China. This study was aimed at searching for environmentally friendly and affordable approaches to increase the production of medicinally relevant glucosinolates and phenolic compounds in Chinese kale plants. For this purpose, the foliar application of liquiritin at 0 (control), 250, 500 and 750 ppm was tested starting from the four‐leaf stage and repeated every two weeks until plants were two months old.RESULTSFoliar application of liquiritin in Chinese kale plants significantly increased glucosinolates and total phenolic content, in a dose‐dependent manner. Compared with control plants, 2.3‐ and 1.9‐fold increases in yields of glucosinolates and total phenolic content, respectively, were corroborated in Chinese kale plants treated with 750 ppm of liquiritin. Along with rises in the content of eight different glucosinolates, liquiritin elicitation effectively increased the concentration of glycosilated and acylated flavonoids and hydroxycinnamic acids. The expression of genes involved in glucosinolate and phenolic biosynthesis was significantly higher in liquiritin‐treated plants as compared to controls.CONCLUSIONSLiquiritin elicitation is a feasible and environmentally friendly practice for increasing the production of medicinally important glucosinolates and phenolic compounds in Chinese kale, which may improve this plant's value as a nutraceutical food. This study also contributes to understanding the molecular mechanisms underlying liquiritin elicitation. This is the first report documenting the use of liquiritin for an elicitation purpose in plants. © 2019 Society of Chemical Industry
Use of laser‐assisted indocyanine green angiography in breast reconstruction: Systematic review and meta‐analysis
Authors: da Silva Neto Edgard , Figueiredo Pedro H. M. , Moro Marcella G. , de Oliveira Ana P. L. , Assumpção Carolina B. , Perina André L. F. , da Costa Fábio P. P. , Faria Eduardo P. , de Oliveira André C. V. , Prates Renato A. 
Published: November 2019

AbstractLaser‐assisted indocyanine green angiography allows surgeons to determine intraoperative flap perfusion and achieve the best outcomes in breast reconstruction. This study stratified outcomes based on a meta‐analysis of complications including longitudinal trials comparing the clinical assessment of skin flaps during breast reconstruction. Nine studies met inclusion criteria and reported outcomes of interest (n = 2256). The risk of flap necrosis and the necessity of reoperation was statistically significantly higher in the control group.
Correlation of sinonasal symptoms with the size and position of nasal septal perforations
Authors: Khong Grace C. , Leong Samuel C. 
Published: November 2019

ObjectiveTo assess the correlation of sinonasal symptoms and quality of life with size and position of nasal septal perforation (NSP).MethodThis is a prospective observational study in a tertiary referral center involving adult patients presented with NSP. The Sino‐Nasal Outcome Test‐22 (SNOT‐22) and its clinico‐psychometric domains were analyzed, including additional NSP‐specific symptoms (nasal crusting, epistaxis, and whistling noise during nasal breathing). The size of NSP was measured radiologically by calculating the area in cm2 and anteroposterior (AP) diameter. Position of perforation was determined clinically by distance from columella to the anterior edge of the perforation.ResultsForty patients were included in this study (22 males). The most common etiology of NSP was iatrogenic. The mean SNOT‐22 score was 50.8 (standard deviation 23.8), and mean NSP size was 3.0 cm2. No statistical correlation was observed between the total SNOT‐22 score with either position or size of NSP. As for NSP‐specific symptoms, there was a statistically significant negative correlation with the size of perforation (A‐P diameter) (r = −0.34, P = 0.03) and position of the perforation (r = −0.49, P = 0.0016), suggesting that these symptoms improved with posterior and larger perforations.ConclusionReported SNOT‐22 scores were comparable to patients with recalcitrant chronic rhinosinusitis, although the scores did not correlate with size and position of NSP. Sinonasal symptoms typically observed in NSP improved with more posteriorly placed and larger sized perforations. This study provides an insight into the quality‐of‐life impact of NSP and affirms the clinical observation that anterior NSP are more symptomatic.Level of Evidence4Laryngoscope, 2019
Intracrystalline Dynamics in Oligomer‐Diluted Poly(Ethylene Oxide)
Authors: Schäfer Mareen , Wallstein Niklas , Schulz Martha , Thurn‐Albrecht Thomas , Saalwächter Kay 
Published: November 2019

AbstractSolid‐state creep, ductility, and drawability are relevant mechanical properties of “crystal‐mobile” polymers, related to a large‐scale chain transport through the crystal, which is in turn mediated by intracrystalline monomer jumps. Here, high‐Mw poly(ethylene oxide) is used as a well‐controlled model system, modulating the properties of the amorphous phase by diluting with a non‐crystallized oligomer. Faster intracrystalline motions are found upon oligomer addition, indicating little changes in the fold surface and a dominant influence of the somewhat reduced lamellar thickness.
Effects of Blended Reversible Epoxy Domains on Structures and Properties of Self‐Healing/Shape‐Memory Thermoplastic Polyurethane
Authors: Chen Tianqi , Fang Liang , Lu Chunhua , Xu Zhongzi 
Published: November 2019

AbstractFew thermoplastic polyurethane (TPU) blending materials are reported to tune shape‐memory capability, self‐healing ability, and recyclability as well as mechanical property due to the different requirement of phase morphologies. This work focuses on how reversible epoxy domains affect the structures and properties of TPUs that contain disulfide bonds in main chains. The blended epoxy oligomers with dangling furan groups are miscible with the TPU. Self‐healing efficiency can be improved by such miscible epoxy oligomers that are also beneficial for shape recovery but harmful for shape fixation. In the presence of bis(4‐maleimidophenyl)methane (BMI), crosslinked epoxy domains phase separate from the TPU matrix to form microscale domains after the Diels–Alder (DA) reaction between furan groups and maleimide groups in BMI. Elastic modulus and tensile strength of TPU are greatly improved in comparison with pristine TPU and TPU/epoxy blends without BMI. The phase‐separated domains deteriorate the self‐healing, and the presence of phase‐separated microdomains facilitates the shape fixation but deteriorates the shape recovery. This work is not only useful to further understand the relation between structures of polymer blends with intelligent features, but also offers a useful approach to adjust the properties and capabilities of TPU in a cost‐effective manner.
Real‐time MR elastography for viscoelasticity quantification in skeletal muscle during dynamic exercises
Authors: Schrank Felix , Warmuth Carsten , Görner Steffen , Meyer Tom , Tzschätzsch Heiko , Guo Jing , Uca Yavuz Oguz , Elgeti Thomas , Braun Jürgen , Sack Ingolf 
Published: November 2019

PurposeTo develop and test real‐time MR elastography for viscoelastic parameter quantification in skeletal muscle during dynamic exercises.MethodsIn 15 healthy participants, 6 groups of lower‐leg muscles (tibialis anterior, tibialis posterior, peroneus, extensor digitorum longus, soleus, gastrocnemius) were investigated by real‐time MR elastography using a single‐shot, steady‐state spiral gradient‐echo pulse sequence and stroboscopic undersampling of harmonic vibrations at 40 Hz frequency. One hundred and eighty consecutive maps of shear‐wave speed and loss angle (φ) covering 30.6 s of total acquisition time at 5.9‐Hz frame rate were reconstructed from 360 wave images encoding 2 in‐plane wave components in an interleaved manner. The experiment was carried out twice to investigate 2 exercises—isometric plantar flexion and isometric dorsiflexion—each performed over 10 s between 2 resting periods.ResultsActivation of lower‐extremity muscles was associated with increasing viscoelastic parameters shear‐wave speed and φ, both reflecting properties related to the transverse direction relative to fiber orientation. Major viscoelastic changes were observed in soleus muscle during plantar flexion (shear‐wave speed: 20.0% ± 3.6%, φ: 41.3% ± 12.0%) and in the tibialis anterior muscle during dorsiflexion (41.8% ± 10.2%, φ: 27.9% ± 2.8%; all P < .0001). Two of the muscles analyzed were significantly activated by plantar flexion and 4 by dorsiflexion based on shear‐wave speed, whereas φ changed significantly in 5 muscles during both exercises.ConclusionReal‐time MR elastography allows mapping of dynamic, nonperiodic viscoelasticity changes in soft tissues such as voluntary muscle with high spatial and temporal resolution. Real‐time MR elastography thus opens new horizons for the in vivo study of physiological processes in soft tissues toward functional elastography.
Nusinersen for older patients with spinal muscular atrophy: A real‐world clinical setting experience
Authors: Veerapandiyan Aravindhan , Eichinger Katy , Guntrum Debra , Kwon Jennifer , Baker Lindsay , Collins Erin , Ciafaloni Emma 
Published: November 2019

AbstractIntroductionClinical trials data concerning use of nusinersen in older spinal muscular atrophy (SMA) patients is lacking. We describe our center's experience in using intrathecal nusinersen for older patients in the clinical setting.MethodsRetrospective study.ResultsTwelve patients (12–52 years old) were treated with nusinersen. Mean follow‐up duration was 17.4 months (range, 4–26 months). All patients had scoliosis; 10 had spinal fusion/instrumentation. All procedures (30 cervical and 57 lumbar punctures) were technically successful. The only side effects were postprocedural headache (9%) and site pain (5.7%). Functional assessments showed stability in 6/9 patients and improvement in 3/9 patients. Subjective improvements in endurance, hand strength, and bulbar functioning critical for activities of daily living were reported in 8/12 patients. None of the patients has discontinued treatment so far.DiscussionIntrathecal nusinersen can be safely delivered in older SMA patients. Available functional outcome measures are not adequate to capture meaningful subjective improvements.
Improving the barrier function of damaged cultured urothelium using chondroitin sulfate
Authors: Rozenberg Boy. B. , Janssen D. A. W. , Jansen C. F. J. , Schalken J. A. , Heesakkers J. P. F. A. 
Published: November 2019

AbstractAimsTo determine whether glycosaminoglycan (GAG) replenishment is able to improve recovery of a deficient urothelial barrier, chondroitin sulfate (CS) instillations were tested using an in vitro model. Porcine urothelial cells (Ucells) were terminally differentiated in culture conditions to construct a urothelial layer with a functional barrier. This layer was damaged to compromise barrier function to simulate a key characteristic of bladder pain syndrome/interstitial cystitis. The functional effect of subsequent treatment with CS was evaluated.MethodsPrimary porcine Ucells were isolated and cultured on inserts. Differentiation of cells was evaluated with immunohistochemical analysis for the presence of umbrella cells, tight junctions and CS. Transepithelial electrical resistance (TEER) measurements were performed to evaluate barrier function. Protamine was used to simulate mild urothelial damage. CS 0.2% (vol/vol), a GAG, was subsequently instilled in the treatment group. The recovery of barrier function was further evaluated with TEER measurements. The Student t test was used for the analysis of results.ResultsAfter induction of differentiation, the Ucells expressed barrier markers and a functional barrier was established (measured by high TEER). TEER decreased significantly after instillation with protamine. CS instillation improved recovery of TEER significantly measured after 7 hours (84% vs 22% in controls). After 24 hours; however, the TEER was comparable in both experimental groups.ConclusionCS instillation improves the recovery of the urothelial barrier after damage in vitro. This functional experiment shows that CS improves recovery of damaged urothelial function, which supports the hypothesis behind the mechanism of action of GAG‐replenishment therapy.
A comparative descriptive analysis of perceived quality of caring attitudes and behaviours between haemodialysis patients and their nurses
Authors: Delmas Philippe , Antonini Matteo , Berthoud Laurent , O’Reilly Louise , Cara Chantal , Brousseau Sylvain , Bellier‐Teichmann Tanja , Weidmann Jean , Roulet‐Schwab Delphine , Ledoux Isabelle , Pasquier Jérôme , Boillat Evelyne , Brandalesi Vanessa , Konishi Mario 
Published: November 2019

AbstractAimDespite its importance in nursing, perceived quality of the nurse–patient relationship has seldom been researched. This study sought to examine and compare the quality of caring attitudes and behaviours as perceived by haemodialysis patients and their nurses.DesignThis comparative descriptive study involved 140 haemodialysis patients and 101 nurses caring for them in ten haemodialysis units in the French‐speaking part of Switzerland.MethodsParticipants completed a sociodemographic questionnaire and the Caring Nurse‐Patient Interaction Scale (CNPI‐70).ResultsBoth nurses and patients reported a high frequency of caring attitudes and behaviours. Patients gave higher ratings than nurses did on all the caring dimensions, except spirituality. Implications are discussed.