bluuebunny/crossref_metadata_2025_split huggingface dataset:
Culturable gut bacteria of Ikan Batak (Neolissochilus sumatranus Weber & de Beaufort, 1916) collected in Toba Samosir, Indonesia
Authors: Dinoto Achmad , Handayani Rini , Setianingrum Ninu , Julistiono Heddy 
Published: November 2020

Abstract. Dinoto A, Handayani R, Setianingrum N, Julistiono H. 2020. Culturable gut bacteria of Ikan Batak (Neolissochilus sumatranus Weber & de Beaufort, 1916) collected in Toba Samosir, Indonesia. Biodiversitas 21: 4483-4488. Ikan Batak (Neolissochilus sumatranus Weber & de Beaufort, 1916) is one of the fish species that is rarely found in water and have the status of endangered species. In consequence, the loss of endemic fish may contribute to the loss of microorganisms that inhabits the fish as a host. The studies on microorganisms associated with N. sumatranus are very limited. Therefore, the purpose of this study was to isolate and identify the culturable bacteria isolated from the gut of N. sumatranus. Sampling of N. sumatranus was carried out in a river within Toba Samosir area which flows to Lake Toba. Fish gut content was collected for isolating microorganisms using three media, including MRS, 10X diluted MRS, and MRS supplemented with 1% bile salt. Thirteen isolates were successfully isolated and identified based on 16S rRNA. This study revealed various species of gut bacteria recovered from N. sumatranus based on BLAST analysis. The isolates showed closest relationship to species Bacillus subtilis (3 isolates), Bacillus tequilensis (2 isolates), Tumebacillus ginsengisoli (6 isolates), Klebsiella pneumoniae (1 isolate), and Lactobacillus pentosus (1 isolate) with the similarity ranging at 98.7 to 100%. All 16S rRNA gene nucleotides of isolates have been submitted to GenBank. This study also described the isolates that have a very close relationship with Bacillus tequilla and Bacillus subtilis. Further identification is challenged to obtain a big picture of the diversity of microorganisms and the functionality in the digestive ecosystem of N. sumatranus for their conservation and bioprospecting of microbial-based aquaculture.
Resistance level of several soybean lines of M6 generation to stem rot disease Athelia rolfsii
Authors: Hanafiah Diana Sofia , SAFNI IRDA , SIREGAR LUTHFI A.M. , DAMANIK REVANDY I.M. , LESTAMI ANGGRIA , MATONDANG MIKA 
Published: November 2020

Abstract. Hanafiah DS, Safni I, Siregar LAM, Famanik RIM, Lestmi A, Matondang M. 2020. Resistance level of several soybean lines of M6 generation to stem rot disease Athelia rolfsii. Biodiversitas 21: 4537-4542. Athelia rolfsii (Curzi) C.C. Tu & Kimbr. is a soil-borne pathogen that causes stem rot disease on plants. The attack is one of the causes of low soybean productivity hence induction of plant resistance is needed to overcome these problems. Mutagenesis is one of the breeding techniques for inducing genetic variability especially for disease-resistant characters in soybean. This research aimed to obtain selected soybean plant lines (Glycine max L. Merr) of M6 generation based on resistant character against stem rot disease A. rolfsii. This research was conducted at the Laboratory of Plant Disease and research field of Faculty of Agriculture, Universitas Sumatera Utara, Medan, Indonesia and soybean planting was carried out on agricultural field, Universitas Sumatera Utara from March to September 2018. This research used augmented design. The treatments were 8 mutant genotypes derived from radiation mutagenesis, that is; Anjasmoro, Argomulyo, and Kipas Putih varieties. The results showed that the analysis of resistant levels on M6 generation based on disease incidence resulted in 2 lines with resistant criteria to stem rot disease A. rolfsii, that is; M100A25 (5/3) and M200A11 (32/3). Observation of intensity of disease attacks showed that Anjasmoro, Argomulyo, M100A6 (31/1), and M200A12 (6/5) had the lowest resistance level compared to other genotypes. The mutant lines that had high category level of resistance are candidates for resistance lines against stem rot disease A. rolfsii.
Short Communication: Variations of morphology, anatomy, and metabolite profiles of Citrus reticulata Blanco cv. Tawangmangu grafts produced by shoot tip grafting using several rootstocks
Authors: Einstivina Nuryandani  , Susandarini Ratna , Ari Indrianto  , Tri Rini Nuringtyas  , Artnice Mega Fathima  , Siti Subandiyah  
Published: November 2020

Abstract. Nuryandani E, Susandarini R, Indrianto A, Nuringtyas RT, Fathima AM, Subandiyah S. 2020. Short Communication: Variations of morphology, anatomy, and metabolite profiles of Citrus reticulata Blanco cv. Tawangmangu grafts produced by shoot tip grafting using several rootstocks. Biodiversitas 21: 4671-4676. Cultivation of Citrus reticulata Blanco cv. Tawangmangu, one of leading mandarin cultivars in Indonesia, remains challenging due to the limitations of suitable rootstocks. The use of suitable rootstocks will greatly determine the success of grafting and affect the performance of the scion. This study aimed to evaluate the morphological, anatomical, and metabolic profiles of grafts between Tawangmangu and rootstocks from various species using shoot tip grafting. Observations of morphological, anatomical, and metabolite variation were carried out eight weeks after the beginning of the growth of the scions. The morphological characters were observed on leaves and stems of the scions. Anatomical data were obtained from the examination of longitudinal sections of grafts prepared using paraffin-embedded method. Analysis of metabolite profiles was performed using LC-MS. The results showed that for two of the three rootstocks used, C. ichangensis × C. reticulata cv. Austera (Japansche Citroen (JC)) and Citrus (Fortunella) japonica (Fortunella) scion growth was vigorous and resulted in a large number of leaves and long stems. Morphological variation was found in the leaf shape, as indicated by wider and wavy leaves of grafts with Fortunella, while narrower and straighter leaves were found in grafts with JC. The grafting onto the third rootstock, C. hystrix, induced dwarfing of the scions. Variation in the anatomical profiles was found in vascular vessels, and in exodermic and endodermic cell sizes. Differences in metabolic profiles of the grafts were found compared to scion and rootstocks. Results of this study provide an important contribution to the successful improvement of Tawangmangu.
Biology, morphology and damage of the lesser Coconut weevil, Diocalandra frumenti (Coleoptera: Curculionidae) in southern Vietnam
Authors: Nguyen Ung , NGUYEN THI-HIEN , CHAU NGUYEN-QUOC-KHANH , LE VAN-VANG , TRAN VAN-HAI 
Published: November 2020

Abstract. Nguyen HU, Nguyen TH, Chau NQK, Le VV, Tran VH. 2020. Biology, morphology and damage of the lesser coconut weevil, Diocalandra frumenti (Coleoptera: Curculionidae) in southern Vietnam. Biodiversitas 21: 4686-4694. The lesser coconut weevil, Diocalandra frumenti, is an emerging pest of coconut trees in Vietnam. To help develop control options for D. frumenti, this study investigated its morphological and biological characteristics, and quantified damage levels on coconut trees. Results from the study showed that attack by D. frumenti on coconut trees is correlated with characteristic damage symptoms (e.g., oozing sap) on all maturity stages of coconut fruits throughout the year (24.7% infestation), with higher damage levels on young fruits (57.6%). Results also showed that infestation levels on trees (58.9%), coconut bunches (19.4%), and fruits (7.77%) varied greatly. Adults have four different morphologies, but genetic study showed that they are all one species. The life cycle from egg to adult averaged 167± 34.3 days. The average development time for eggs, larvae, female pupae, male pupae, male adults, and female adults was 5.62 ± 0.62, 142.3 ± 34.3, 9.79 ± 0.86, 10.2 ± 1.26, 81.5 ± 34.7, and 81.8 ± 37.2 days, respectively. In conclusion, D. frumenti is an important pest in coconut trees with significant fluctuations in the duration of its development stages, and variation in shape, color, and size of the eggs, larvae, pupae, and adults. They can attack any coconut orchard and have harmful impacts on coconut trees, coconut bunches, and fruits throughout the year.
Prevalence of carbapenem-resistant Enterobacteriaceae and emergence of high rectal colonization rates of blaOXA-181-positive isolates in patients admitted to two major hospital intensive care units in Kuwait
Authors: Al Fadhli Amani H. , Jamal Wafaa Y. , Rotimi Vincent O. 
Published: November 2020

BackgroundFecal colonization by carbapenem-resistant Enterobacteriaceae (CRE) can be the main reservoir for transmission of these resistant organisms especially in the Intensive Care Units (ICUs).AimThis study was conducted to evaluate the rate of rectal carriage and molecular characterization of CRE in patients hospitalized in the ICUs of 2 major hospitals (Adan and Mubarak Al Kabeer Hospitals) in Kuwait.Materials and methodsRectal swabs were collected from all patients at admission, 48 h after admission and once weekly from April 2017- March 2018. Initial CRE screening was carried out on MacConkey agar on which meropenem disc 10μg was placed. Identification of isolates was by API 20E. Susceptibility testing was performed using the E-test method. Polymerase chain reaction (PCR) was used to detect the carbapenemase-encoding genes. Clonal relationship was investigated by pulsed-field electrophoresis (PFGE). Genes ofblaOXA-181andblaNDM-5–carrying plasmids were detected in some strains.ResultsA total of 590 patients were recruited into the study. Of these, 58 were positive for CRE, giving a prevalence of 9.8%; 25/320 (7.8%) in Adan and 33/270 (12.2%) in Mubarak Al Kabeer Hospitals. All isolates were resistant to multiple antibiotics. Resistance rates to colistin and tigecycline were 17% and 83%, respectively. Single genes ofblaOXA-181were detected in isolates from 38 (65.5%) out of 58 patients and in 5 patients colonized byblaOXA-48-positive CRE. A combination of 2 genes was detected in 12 isolates; 5blaKPC-2andblaOXA-181, 4blaVIM-1andblaOXA-181, and 3blaNDM-5andblaOXA-181. PFGE showed an overall level of similarity of 38%. Southern hybridization studies localized theblaOXA-181andblaNDM-5genes to a large plasmid of 200kb in 3K.pneumoniaeisolates and a small plasmid of 80kb in 2E.coliisolates, respectively.ConclusionThe prevalence of CRE colonization in the 2 hospital ICUs was relatively high and the emergence ofblaOXA-181-mediated CRE is a cause for concern as there is the possibility of rapid horizontal spread among hospital patients in Kuwait. Active surveillance of CRE in the ICUs is highly recommended to stem its spread.
Implementation fidelity of school oral health programs at a District in South Africa
Authors: Molete Mpho , Stewart Aimee , Igumbor Jude 
Published: November 2020

BackgroundIt is important that components contributing to success of a program are well understood to ensure better outcomes and strengthen interventions. Hence the purpose of the study was to assess the level of fidelity achieved by school oral health programs in our study district and to determine elements of fidelity that predict the risk of dental decay.MethodsA cross-sectional study design was utilised. A multistage sampling technique was employed to randomly select 10 schools, two grades in each school were selected and all pupils in the selected grades were included in an oral health examination. Ten oral hygienists were observed and interviewed as they carried out the activities of the program and records were reviewed. Data collection tools included an oral health examination form, and an implementation fidelity checklist.ResultsThe average level of fidelity obtained was 40% and it was shown to be inversely correlated with levels of decay, as decay was predicted to decrease with increasing levels of fidelity. The fidelity elements that were found to directly predict the outcome of decay included duration (IRR, 0.49; p = 0.02) coverage (IRR, 0.54; p = 008), content (IRR, 1.36; p = 0.03) and age (IRR, 2.14; p = 0.00). Moderating factors of fidelity which indirectly influenced the outcome of decay included facilitation strategy, duration and age. These were predicted to reduce the risk of decay by 92%, 83% and 48% respectively.ConclusionThe school oral health programs exhibited high levels of pupil coverage, however, the content of the programs offered was low (28%). Coverage was high in the context of lack of dental assistance and time. Multi-sectoral participation is therefore necessary to re-organise the program for improving implementation fidelity and bringing about quality implementation.
Association between maternal satisfaction with breastfeeding and postpartum depression symptoms
Authors: Avilla Juliana Castro de , Giugliani Camila , Bizon Agnes Meire Branco Leria , Martins Ana Cláudia Magnus , Senna Andrea Francis Kroll de , Giugliani Elsa Regina Justo 
Published: November 2020

Background
Due to the multiple health benefits of breastfeeding, it is essential to identify factors that may negatively interfere with this healthy practice. Among such factors are postpartum depression (PPD) and maternal satisfaction with breastfeeding. The objective of this study was to evaluate the association between maternal satisfaction with breastfeeding and symptoms of PPD in the first month after childbirth.

Methods
This cross-sectional study nested in a cohort study was conducted in Porto Alegre, Brazil, with 287 puerperal women selected at two maternity hospitals, one public and one private. Women were interviewed at their homes the week after the infant completed 30 days of life. A structured questionnaire was applied, as well as instruments to evaluate maternal satisfaction with breastfeeding (Maternal Breastfeeding Evaluation Scale) and to screen for PPD (Edinburgh Postnatal Depression Scale). The association between higher satisfaction with breastfeeding (outcome) and negative PPD screening test was assessed using Poisson regression with robust variance, adjusting for specific covariables. Adjusted prevalence ratios (aPR) and respective 95% confidence intervals (95%CI) were estimated.

Results
The prevalence of increased satisfaction with breastfeeding (defined as women with scores above the median) was 47% higher among women who screened negative for PPD when compared to those with a positive result (aPR 1.47; 95%CI 1.01–2.16). This result was adjusted for maternal age and skin color, cohabitation with the infant’s father, planned pregnancy, type of delivery, exclusive breastfeeding, and occurrence of breastfeeding problems.

Conclusions
The findings of this study showed an association between higher maternal satisfaction with breastfeeding and absence of PPD symptoms, reinforcing the importance of caring for the mental health of pregnant and puerperal women and paying attention to their satisfaction with breastfeeding.
A novel deep learning-based point-of-care diagnostic method for detecting Plasmodium falciparum with fluorescence digital microscopy
Authors: Holmström Oscar , Stenman Sebastian , Suutala Antti , Moilanen Hannu , Kücükel Hakan , Ngasala Billy , Mårtensson Andreas , Mhamilawa Lwidiko , Aydin-Schmidt Berit , Lundin Mikael , Diwan Vinod , Linder Nina , Lundin Johan 
Published: November 2020

BackgroundMalaria remains a major global health problem with a need for improved field-usable diagnostic tests. We have developed a portable, low-cost digital microscope scanner, capable of both brightfield and fluorescence imaging. Here, we used the instrument to digitize blood smears, and applied deep learning (DL) algorithms to detectPlasmodium falciparumparasites.MethodsThin blood smears (n= 125) were collected from patients with microscopy-confirmedP.falciparuminfections in rural Tanzania, prior to and after initiation of artemisinin-based combination therapy. The samples were stained using the 4′,6-diamidino-2-phenylindole fluorogen and digitized using the prototype microscope scanner. Two DL algorithms were trained to detect malaria parasites in the samples, and results compared to the visual assessment of both the digitized samples, and the Giemsa-stained thick smears.ResultsDetection ofP.falciparumparasites in the digitized thin blood smears was possible both by visual assessment and by DL-based analysis with a strong correlation in results (r = 0.99, p < 0.01). A moderately strong correlation was observed between the DL-based thin smear analysis and the visual thick smear-analysis (r = 0.74, p < 0.01). Low levels of parasites were detected by DL-based analysis on day three following treatment initiation, but a small number of fluorescent signals were detected also in microscopy-negative samples.ConclusionQuantification ofP.falciparumparasites in DAPI-stained thin smears is feasible using DL-supported, point-of-care digital microscopy, with a high correlation to visual assessment of samples. Fluorescent signals from artefacts in samples with low infection levels represented the main challenge for the digital analysis, thus highlighting the importance of minimizing sample contaminations. The proposed method could support malaria diagnostics and monitoring of treatment response through automated quantification of parasitaemia and is likely to be applicable also for diagnostics of otherPlasmodiumspecies and other infectious diseases.
A test-retest reliability study of assessing small cutaneous fibers by measuring current perception threshold with pin electrodes
Authors: Xia Weiwei , Fu Han , Liu Haiying , Meng Fanqi , Wang Kaifeng 
Published: November 2020

Background
The quantitative measurement of current perception threshold (CPT) has been used as a method to assess the function of nerve fibers in neuropathy diseases. The aim of this study was to assess the test-retest reliability measuring CPT using the circular pin electrodes for assessing the function of cutaneous thin nerve fibers.

Methods
CPT measurement was repeated on two separate days with at least one-week interval in 55 volunteers. Superficial blood flow (SBF) and skin temperature (ST) were measured on the skin in an around area concentric to the circular pin electrodes after the process of finding CPTs. The coefficient of variation (CV) and intra-class correlation coefficient (ICC) were calculated. The correlation between each two of CPT, SBF increment and ST increment was analyzed.

Results
No significant differences were found for CPT, SBF and ST between two sessions. SBF was found to be significantly increased after the process of finding CPT. CPT values of males were found to be higher than females. SBF increment was found to be positively correlated with ST increment. The ICC values for CPT, SBF and ST were 0.595, 0.852 and 0.728, respectively. The CV values for CPT, SBF and ST were 25.53%, 12.59% and 1.94%, respectively.

Conclusions
The reliability of CPT measurement using circular pin electrodes is fair, and need consistence of measurements in longitudinal studies.
Rancang Bangun Sistem Informasi Manajemen Proyek Departemen IT PT. Pertamina UPMS V Surabaya
Authors: Ardilla Yunita , Pramesti Shinta , Sunaryono Dwi 
Published: November 2020

Department of IT M&T PT. Pertamina UPMS V Surabaya has currently implemented a management guideline based on COBIT 5.0 to support the process of monitoring and scheduling projects that will be implemented or currently being worked on PT. Pertamina UPMS V Surabaya. But in the process of implementing management guidelines, the department of IT M&T has not maximized the implementation of the monitoring and scheduling because it does not yet have a project management information system application. So far employees from the department of IT M&T use the process of monitoring and scheduling projects manually, namely by making a report in the form of an excel file for each progress of the projects. Of course, this is considered inefficient and often encounters human errors. Therefore, a web-based project management information system application was made, the application was built using the C3 programming language and Microsoft SQL Server database. Based on observation, it was concluding that the features available in the project management information system application had been running well and were able to meet the needs of users.
Analisa Usability Website Akademi Komunitas Negeri Pacitan Menggunakan Metode Heuristic Evaluation
Authors: Nugroho Kurnianto Tri , Julianto Bagus , Intyanto Gramandha Wega , Munir Mochammad Syahrul , Nur M.S. Danny Febryan 
Published: November 2020

The development of information technology today provides many changes to human life, one of which is the website. The website of the university in particular can help the wider community, especially students, in obtaining information. Akademi Komunitas Negeri (AKN) Pacitan is one of the many colleges that utilize information technology in the form of websites. To find out whether the website created has made it easy for users, it is necessary to analyze the usability of the website. This study will analyze the level of usability on the AKN Pacitan website with the Heuristic Evaluation method. The variables used in this study are five usability variables of the ten variables in the Heuristic Evaluation method, including visibility of system status, the match between system and the real world, consistency and standards, flexibility and efficiency of use, and aesthetic and minimalist design. The population of this research is students, lecturers, and AKN Pacitan staff, using probability sampling, simple random sampling, and proportionate stratified random sampling as data collection techniques. The research data was taken using a questionnaire and analyzed using descriptive analysis methods. From the results of the subsequent questionnaire, the analysis will produce several recommendations for improvement and further development of the AKN Pacitan website to improve the functionality of the website itself.