bluuebunny/crossref_metadata_2025_split huggingface dataset:
Therapeutic potential of CKD-506, a novel selective histone deacetylase 6 inhibitor, in a murine model of rheumatoid arthritis
Authors: Park Jin Kyun , Jang Yu Jin , Oh Bo Ram , Shin Jieun , Bae Daekwon , Ha Nina , Choi Young il , Youn Gi Soo , Park Jinseu , Lee Eun Young , Lee Eun Bong , Song Yeong Wook 
Published: July 2020

AbstractObjectivesHistone deacetylase (HDAC) 6 promotes inflammation. We investigated the anti-arthritic effects of CKD-506, a novel HDAC6 inhibitor, in vitro and in a murine model of arthritis as a novel treatment option for rheumatoid arthritis (RA).MethodsHDAC6 was overexpressed in mouse peritoneal macrophages and RAW 264.7 cells, and the effects of a HDAC6 inhibitor CKD-506 on cytokine production and activity of NF-κB and AP-1 signaling were examined. Peripheral blood mononuclear cells (PBMCs) from RA patients and fibroblast-like synoviocytes (FLS) were activated in the presence of CKD-506. Next, regulatory T cells (Tregs) were induced from RA patients and co-cultured with healthy effector T cells (Teffs) and cell proliferation was analyzed by flow cytometry. Finally, the effects of the inhibitor on the severity of arthritis were assessed in a murine model of adjuvant-induced arthritis (AIA).ResultsOverexpression of HDAC6 induced macrophages to produce TNF-α and IL-6. The inhibitory effect of CKD-506 was mediated via blockade of NF-κB and AP-1 activation. HDAC6 inhibition reduced TNF-α and IL-6 production by activated RA PBMCs. CKD-506 inhibited production of MMP-1, MMP-3, IL-6, and IL-8 by activated FLS. In addition, CKD-506 inhibited proliferation of Teffs directly and indirectly by improving iTreg function. In AIA rats, oral CKD-506 improved clinical arthritis in a dose-dependent manner. A combination of sub-therapeutic CKD-506 and methotrexate exerted a synergistic effect.ConclusionThe novel HDAC6 inhibitor CKD-506 suppresses inflammatory responses by monocytes/macrophages, improves Treg function, and ameliorates arthritis severity in a murine model of RA. Thus, CKD-506 might be a novel and effective treatment option for RA.
The effect of increasing heel height on lower limb symmetry during the back squat in trained and novice lifters
Authors: Sayers Mark G. L. , Hosseini Nasab S. H. , Bachem Caroline , Taylor William R. , List Renate , Lorenzetti Silvio 
Published: July 2020

Abstract
Background
Symmetry during lifting is considered critical for allowing balanced power production and avoidance of injury. This investigation assessed the influence of elevating the heels on bilateral lower limb symmetry during loaded (50% of body weight) high-bar back squats.

Methods
Ten novice (mass 67.6 ± 12.4 kg, height 1.73 ± 0.10 m) and ten regular weight trainers (mass 66.0 ± 10.7 kg, height 1.71 ± 0.09 m) were assessed while standing on both the flat level floor and on an inclined board. Data collection used infra-red motion capture procedures and two force platforms to record bilateral vertical ground reaction force (GRFvert) and ankle, knee and hip joint kinematic and kinetic data. Paired t-tests and statistical parametric mapping (SPM1D) procedures were used to assess differences in discrete and continuous bilateral symmetry data across conditions.

Results
Although discrete joint kinematic and joint moment symmetry data were largely unaffected by raising the heels, the regular weight trainers presented greater bilateral asymmetry in these data than the novices. The one significant finding in these discrete data showed that raising the heels significantly reduced maximum knee extension moment asymmetry (P = 0.02), but in the novice group only. Time-series analyses indicated significant bilateral asymmetries in both GRFvert and knee extension moments mid-way though the eccentric phase for the novice group, with the latter unaffected by heel lift condition. There were no significant bilateral asymmetries in time series data within the regular weight training group.

Conclusions
This investigation highlights that although a degree of bilateral lower limb asymmetry is common in individuals performing back squats, the degree of this symmetry is largely unaffected by raising the heels. Differences in results for discrete and time-series symmetry analyses also highlight a key issue associated with relying solely on discrete data techniques to assess bilateral symmetry during tasks such as the back squat.
The effects of intravitreal adalimumab injection on pseudophakic macular edema
Authors: Farvardin Mohsen , Namvar Ehsan , Sanie-Jahromi Fatemeh , Johari Mohammad Karim 
Published: July 2020

Abstract
Objective
Pseudophakic macular edema is a frequent complication following cataract surgery. Inflammation is a major etiologic factor in the development of pseudophakic cystoid macular edema. Tumor necrosis factor-alpha has an important role in ocular inflammation. Adalimumab (Humira) is an inhibitor of tumor necrosis factor-alpha that has been approved in the United States. An open-label, uncontrolled, prospective, interventional study of five consecutive patients (5 eyes) with cystoid macular edema who were treated with off-label intravitreal adalimumab at Khalili Hospital was conducted. Slit-lamp examination and optical coherence tomography were done for all patients.

Results
No statistically significant difference was detected between best corrected visual acuity and central macular thickness before and after injection in pseudophakic macular edema. One patient developed uveitis approximately 2 weeks after injection. Based on the results, adalimumab does not appear to be an effective treatment for pseudophakic macular edema, and it may cause uveitis. Caution should be exercised when using this drug.
Trial registration Iranian Registry of Clinical Trials IRCT2016100430130N1, 2016.12.03, Retrospectively registered
Effects of different abiotic stresses on carotenoid and fatty acid metabolism in the green microalga Dunaliella salina Y6
Authors: Wu Mingcan , Zhu Rongfang , Lu Jiayang , Lei Anping , Zhu Hui , Hu Zhangli , Wang Jiangxin 
Published: July 2020

Abstract
Purpose
Under different abiotic-stress conditions, the unicellular green microalga Dunaliella salina accumulates large amounts of carotenoids which are accompanied by fatty acid biosynthesis. Carotenoids and fatty acids both possess long carbon backbones; however, the relationship between carotenoid and fatty acid metabolism is controversial and remains poorly understood in microalgae.

Methods
In this study, we investigated the growth curves and the β-carotene, lutein, lipid, and fatty acid contents of D. salina Y6 grown under different abiotic-stress conditions, including high light, nitrogen depletion, and high salinity.

Results
Both high-salinity and nitrogen-depleted conditions significantly inhibited cell growth. Nitrogen depletion significantly induced β-carotene accumulation, whereas lutein production was promoted by high light. The accumulation of lipids did not directly positive correlate with β-carotene and lutein accumulation under the three tested abiotic-stress conditions, and levels of only a few fatty acids were increased under specific conditions.

Conclusion
Our data indicate that cellular β-carotene accumulation in D. salina Y6 positive correlates with accumulation of specific fatty acids (C16:0, C18:3n3, C14:0, and C15:0) rather than with total fatty acid content under different abiotic stress conditions.
Respective stemness and chondrogenic potential of mesenchymal stem cells isolated from human bone marrow, synovial membrane, and synovial fluid
Authors: Neybecker Paul , Henrionnet Christel , Pape Elise , Grossin Laurent , Mainard Didier , Galois Laurent , Loeuille Damien , Gillet Pierre , Pinzano Astrid 
Published: July 2020

AbstractBackgroundMSCs isolated from bone marrow (BM-MSCs) have well-established chondrogenic potential, but MSCs derived from the synovial membrane (SM-MSCs) and synovial fluid (SF-MSCs) are thought to possess superior chondrogenicity. This study aimed to compare the in vitro immunophenotype and trilineage and chondrogenic potential of BM-MSCs to SM-MSCs and SF-MSCs.MethodsMSCs were isolated from bone marrow (BM-MSCs), synovial membrane (SM-MSCs), and synovial fluid (SF-MSCs) extracted from the hips (BM) and knees (SM and SF) of advanced OA patients undergoing arthroplasty. Flow cytometric analysis was used at P2 to evaluate cell stemness. The trilinear differentiation test was performed at P2. At P3, MSC-seeded collagen sponges were cultured in chondrogenic medium for 28 days. Chondrogenic gene expression was quantified by qRT-PCR. Finally, the implants were stained to assess the deposition of proteoglycans and type II collagen.ResultsDespite variability, the immunophenotyping of BM-MSCs, SM-MSCs, and SF-MSCs was quite similar. All cell types were positive for the expression of stem cell markers and negative for exclusion markers. Additionally, chondrogenic differentiation and hypertrophy were more pronounced in BM-MSCs (ACAN,SOX9,COL2B, andCOL10A) than in SF-MSCs, with SM-MSCs having intermediate characteristics. Concerning matrix synthesis, the three cell types were equipotent in terms of GAG content, while BM-MSC ECM synthesis of type II collagen was superior.ConclusionsChondrogenic MSCs are easily collected from SM and SF in advanced human OA, but in vitro chondrogenesis that is superior to age-matched BM-MSCs should not be expected. However, due to intra-articular priming, SF-MSCs did not overexpress hypertrophic gene.
Transcriptional profiling reveals altered biological characteristics of chorionic stem cells from women with gestational diabetes
Authors: Chen Liyun , Wang Chung-Teng , Forsyth Nicholas R. , Wu Pensee 
Published: July 2020

AbstractBackgroundGestational diabetes (GDM) is a common complication of pregnancy. The impact of pregnancy complications on placental function suggests that extraembryonic stem cells in the placenta may also be affected during pregnancy. Neonatal tissue-derived stem cells, with the advantages of their differentiation capacity and non-invasive isolation processes, have been proposed as a promising therapeutic avenue for GDM management through potential cell therapy approaches. However, the influence of GDM on autologous stem cells remains unclear. Thus, studies that provide comprehensive understanding of stem cells isolated from women with GDM are essential to guide future clinical applications.MethodsHuman chorionic membrane-derived stem cells (CMSCs) were isolated from placentas of healthy and GDM pregnancies. Transcriptional profiling was performed by DNA microarray, and differentially regulated genes between GDM- and Healthy-CMSCs were used to analyse molecular functions, differentiation, and pathway enrichment. Altered genes and biological functions were validated via real-time PCR and in vitro assays.ResultsGDM-CMSCs displayed, vs. Healthy-CMSCs, 162 upregulated genes associated with increased migration ability, epithelial development, and growth factor-associated signal transduction while the 269 downregulated genes were strongly linked to angiogenesis and cellular metabolic processes. Notably, significantly reduced expression of detoxification enzymes belonging to the aldehyde dehydrogenase gene families (ALDH1A1/1A2, ALDH2, ALDH3) accounted for downregulation across several metabolic pathways. ALDH activity and inhibitor assays indicated that reduced gene expression of ALDHs affected ALDH enzymatic functions and resulted in oxidative stress dysregulation in GDM-CMSCs.ConclusionOur combined transcriptional analysis and in vitro functional characterisation have provided novel insights into fundamental biological differences in GDM- and Healthy-CMSCs. Enhanced mobility of GDM-CMSCs may promote MSC migration toward injured sites; however, impaired cellular metabolic activity may negatively affect any perceived benefit.
MicroRNA-503-3p affects osteogenic differentiation of human adipose-derived stem cells by regulation of Wnt2 and Wnt7b under cyclic strain
Authors: Luo Yadong , Ding Xu , Ji Huan , Li Meng , Song Haiyang , Li Sheng , Wang Chenxing , Wu Heming , Du Hongming 
Published: July 2020

Abstract
Background
MicroRNAs (miRNAs) play a role in regulating osteogenic differentiation (OD) of mesenchymal stem cells by inhibiting mRNAs translation under cyclic strain. miR-503-3p was downregulated in OD of human adipose-derived stem cells (hASCs) in vivo under cyclic strain in our previous study, while it might target the Wnt/β-catenin (W-β) pathway. In this study, we explored miR-503-3p’s role in OD of hASCs under cyclic strain.

Methods
OD of hASCs was induced by cyclic strain. Bioinformatic and dual luciferase analyses were used to confirm the relationship between Wnt2/Wnt7b and miR-503-3p. Immunofluorescence was used to detect the effect of miR-503-3p on Wnt2/Wnt7b and β-catenin in hASCs transfected with miR-503-3p mimic and inhibitor. Mimic, inhibitor, and small interfering RNA (siRNA) transfected in hASCs to against Wnt2 and Wnt7b. Quantitative real-time PCR (RT-PCR) and western blot were used to examine the OD and W-β pathway at the mRNA and protein levels, respectively. Immunofluorescence was performed to locate β-catenin. ALP activity and calcium were detected by colorimetric assay.

Results
Results of immunophenotypes by flow cytometry and multi-lineage potential confirmed that the cultured cells were hASCs. Results of luciferase reporter assay indicated that miR-503-3p could regulate the expression levels of Wnt2 and Wnt7b by targeting their respective 3′-untranslated region (UTR). Under cyclic strain, gain- or loss-function of miR-503-3p studies by mimic and inhibitor revealed that decreasing expression of miR-503-3p could significantly bring about promotion of OD of hASCs, whereas increased expression of miR-503-3p inhibited OD. Furthermore, miR-503-3p high-expression reduced the activity of the W-β pathway, as indicated by lowering expression of Wnt2 and Wnt7b, inactive β-catenin in miR-503-3p-treated hASCs. By contrast, miR-503-3p inhibition activated the W-β pathway.

Conclusions
Collectively, our findings indicate that miR-503-3p is a negative factor in regulating W-β pathway by Wnt2 and Wnt7b, which inhibit the OD of hASCs under cyclic strain.
Extrinsic factors influencing gut microbes, the immediate consequences and restoring eubiosis
Authors: Bajinka Ousman , Tan Yurong , Abdelhalim Khalid A. , Özdemir Güven , Qiu Xiangjie 
Published: July 2020

AbstractFrom the emerging studies, the more diverse the microbial population in the gut, the healthier the gut. Health benefits are associated with the functional characteristics of these diverse microbial genes. Extrinsic factors causing dysbiosis are extensively studied however, linking the varying degree of consequences to the respective factors and therapeutic possibilities are not explored at length. This review aims to examine from previous studies and put forward the types of dysbiosis, the immediate consequences and the scientific approaches to restore disrupted microbiota. Dietary supplements are found to be one of the factors contributing profoundly to the alteration of gut microbiota. While diet rich in fibre and fermented food established a diverse microbiome and produce vital metabolites, high fat, animal proteins and high caloric carbohydrate are as well relative to dysbiosis among infants, adult or diseases individuals. The intermittent fasting, feeding methods, the pH and water quality are among the factors associated with dysbiosis. Prebiotics and Probiotics maintain and restore gut homeostasis. Antibiotic-induced dysbiosis are relatively on the spectrum of activity, the pharmacokinetics properties, the dose taken during the treatment route of administration and the duration of drug therapy. The higher the altitude, the lesser the diversity. Extreme temperatures as well are related to reduced microbial activity and metabolism. Delivery through caserium-section deprived the newborn from restoring valuable vaginal bacterial species and the baby will instead assumed intestinal microbiota-like. While exercise and oxidative stress contribute even though moderately, fecal microbial transfer (FMT) also influence gut microbiota.
Volcanic unrest scenarios and impact assessment at Campi Flegrei caldera, Southern Italy
Authors: Charlton Danielle , Kilburn Christopher , Edwards Stephen 
Published: July 2020

AbstractAt Campi Flegrei caldera, volcanic unrest hazards during 1982–1984 caused significant building damage and led to the evacuation of over 40,000 residents in the central town of Pozzuoli. Past hazard assessments in this region have focused on eruption hazards rather than on hazards from volcanic unrest. In this study, we developed a hypothetical unrest hazard scenario applied to three locations within Campi Flegrei caldera: Pozzuoli, Agnano and Baia. We also collated GIS exposure datasets and used vulnerability models of building and road damage to carry out an impact assessment for future volcanic unrest at these three test locations. The resulting impact maps provide useful insight into the effects future unrest could have on businesses, buildings and livelihoods within Campi Flegrei.The impact maps show that, depending on the location of unrest, evacuations associated with future unrest may involve displacement of large numbers of residents, and significant damage to buildings and infrastructure. The Agnano scenario is associated with the greatest impact and involves the potential evacuation of 136,000 people and damage to about 2400 buildings, with up to 200 km of roads and 17 km of high-voltage electricity network also exposed. Agnano also lies between Naples and the rest of Campi Flegrei, so that damage to infrastructure may trigger a cascade of obstacles to managing evacuations and repairs during an emergency. The results highlight how a simple impact assessment can be used to explore the possible effects of future unrest hazard, and the importance of considering scenarios in which renewed ground uplift is not necessarily focused beneath Pozzuoli.
New quantum estimates in the setting of fractional calculus theory
Authors: Rashid Saima , Hammouch Zakia , Ashraf Rehana , Baleanu Dumitru , Nisar Kottakkaran Sooppy 
Published: July 2020

AbstractIn this article, the investigation is centered around the quantum estimates by utilizing quantum Hahn integral operator via the quantum shift operator${}_{\eta}\psi_{\mathfrak{q}}(\zeta)=\mathfrak{q}\zeta+(1-\mathfrak{q})\eta$ψqη(ζ)=qζ+(1−q)η,$\zeta\in[\mu,\nu]$ζ∈[μ,ν],$\eta=\mu+\frac{\omega}{(1-\mathfrak{q})}$η=μ+ω(1−q),$0<\mathfrak{q}<1$0<q<1,$\omega\geq0$ω≥0. Our strategy includes fractional calculus, Jackson’s$\mathfrak{q}$q-integral, the main ideas of quantum calculus, and a generalization used in the frame of convex functions. We presented, in general, three types of fractional quantum integral inequalities that can be utilized to explain orthogonal polynomials, and exploring some estimation problems with shifting estimations of fractional order$\varrho_{1}$ϱ1and the$\mathfrak{q}$q-numbers have yielded fascinating outcomes. As an application viewpoint, an illustrative example shows the effectiveness of$\mathfrak{q}$q,ω-derivative for boundary value problem.
Existence of solution and stability for the fractional order novel coronavirus (nCoV-2019) model
Authors: Hussain Azhar , Baleanu Dumitru , Adeel Muhammad 
Published: July 2020

AbstractThe aim of this work is to present a new fractional order model of novel coronavirus (nCoV-2019) under Caputo–Fabrizio derivative. We make use of fixed point theory and Picard–Lindelöf technique to explore the existence and uniqueness of solution for the proposed model. Moreover, we explore the generalized Hyers–Ulam stability of the model using Gronwall’s inequality.
Microbiota from alginate oligosaccharide-dosed mice successfully mitigated small intestinal mucositis
Authors: Zhang Pengfei , Liu Jing , Xiong Bohui , Zhang Cong , Kang Beining , Gao Yishan , Li Zengkuan , Ge Wei , Cheng Shunfeng , Hao Yanan , Shen Wei , Yu Shuai , Chen Liang , Tang Xiangfang , Zhao Yong , Zhang Hongfu 
Published: July 2020

AbstractBackgroundThe increasing incidence of cancer and intestinal mucositis induced by chemotherapeutics are causing worldwide concern. Many approaches such as fecal microbiota transplantation (FMT) have been used to minimize mucositis. However, it is still unknown whether FMT from a donor with beneficial gut microbiota results in more effective intestinal function in the recipient. Recently, we found that alginate oligosaccharides (AOS) benefit murine gut microbiota through increasing “beneficial” microbes to rescue busulfan induced mucositis.ResultsIn the current investigation, FMT from AOS-dosed mice improved small intestine function over FMT from control mice through the recovery of gene expression and an increase in the levels of cell junction proteins. FMT from AOS-dosed mice showed superior benefits over FMT from control mice on recipient gut microbiotas through an increase in “beneficial” microbes such asLeuconostocaceaeand recovery in blood metabolome. Furthermore, the correlation of gut microbiota and blood metabolites suggested that the “beneficial” microbeLactobacillaleshelped with the recovery of blood metabolites, while the “harmful” microbeMycoplasmatalesdid not.ConclusionThe data confirm our hypothesis that FMT from a donor with superior microbes leads to a more profound recovery of small intestinal function. We propose that gut microbiota from naturally produced AOS-treated donor may be used to prevent small intestinal mucositis induced by chemotherapeutics or other factors in recipients.
Predictive big data analytics for supply chain demand forecasting: methods, applications, and research opportunities
Authors: Seyedan Mahya , Mafakheri Fereshteh 
Published: July 2020

AbstractBig data analytics (BDA) in supply chain management (SCM) is receiving a growing attention. This is due to the fact that BDA has a wide range of applications in SCM, including customer behavior analysis, trend analysis, and demand prediction. In this survey, we investigate the predictive BDA applications in supply chain demand forecasting to propose a classification of these applications, identify the gaps, and provide insights for future research. We classify these algorithms and their applications in supply chain management into time-series forecasting, clustering, K-nearest-neighbors, neural networks, regression analysis, support vector machines, and support vector regression. This survey also points to the fact that the literature is particularly lacking on the applications of BDA for demand forecasting in the case of closed-loop supply chains (CLSCs) and accordingly highlights avenues for future research.
Mining frequent itemsets from streaming transaction data using genetic algorithms
Authors: Bagui Sikha , Stanley Patrick 
Published: July 2020

AbstractThis paper presents a study of mining frequent itemsets from streaming data in the presence of concept drift. Streaming data, being volatile in nature, is particularly challenging to mine. An approach using genetic algorithms is presented, and various relationships between concept drift, sliding window size, and genetic algorithm constraints are explored. Concept drift is identified by changes in frequent itemsets. The novelty of this work lies in determining concept drift using frequent itemsets for mining streaming data, using the genetic algorithm framework. Formulas have been presented for calculating minimum support counts in streaming data using sliding windows. Testing highlighted that the ratio of the window size to transactions per drift was a key to good performance. Getting good results when the sliding window size was too small was a challenge since normal fluctuations in the data could appear to be a concept drift. Window size must be managed in conjunction with support and confidence values in order to achieve reasonable results. This method of detecting concept drift performed well when larger window sizes were used.