bluuebunny/crossref_metadata_2025_split huggingface dataset:
MUC1 as a target for CAR‐T therapy in head and neck squamous cell carinoma
Authors: Mei Zi , Zhang Kai , Lam Alfred King‐Yin , Huang Junwen , Qiu Feng , Qiao Bin , Zhang Yi 
Published: December 2019

AbstractThe modification of chimeric antigen receptor (CAR) endowing T cells with tumor‐specific cytotoxicity induces antitumor immunity. However, the structural characteristics of solid tumors, the loss of specific antigens, and the strong immunosuppressive environment are challenges to treat solid tumors with CAR‐T therapy. The purpose of our study was to find and verify the potentials of CAR‐T therapies for patients with head and neck squamous cell carcinoma (HNSCC). First, we selected MUC1 as our research target and verified its differential expression in cancer tissues and adjacent non‐neoplastic tissues (ANNT). Next, we constructed a second‐generation CAR and validated the cytotoxic function in vitro. In our study, we found that exogenous addition human IL22 recombinant protein could increase the MUC1 expression and enhance the function of T cells. In addition, we constructed a fourth‐generation CAR that secretes IL22. Finally, we verified the antitumor function of two different CAR‐T cells in vitro and in vivo, respectively. CAR‐MUC1‐IL22 T cells were found to have a stronger and more effective cytotoxic function against MUC1 + HNSCC cells. Taken together, these results demonstrate the potential effectiveness of CAR‐T in the treatment of patients with HNSCC and provide evidence‐based of MUC1 + CAR‐T therapy.
Herpetic optic perineuritis presenting as classic abducens nerve palsy
Authors: Abid Muhammad Abbas , Mughal Mustafa , Capelli Peter , Abid Muhammad Bilal 
Published: December 2019

AbstractOptic perineuritis is a rare manifestation of herpes zoster ophthalmicus (HZO). Relative afferent pupillary defect (RAPD) is an important early clue to an impending nerve involvement, and robust clinical examination allows early detection of such rare metachronous manifestation of cutaneous HZ and institution of timely management for such sight‐threatening conditions.
The evaluation of oxidative stress in patients with psoriasis vulgaris and atopic dermatitis by measuring the urinary level of 8‐hydroxy‐2′‐deoxyguanosine
Authors: Shimamoto Junko , Kurokawa Teruo , Tanizaki Hideaki , Moriwaki Shinichi 
Published: December 2019

AbstractObjectivesRecent studies have revealed the relationship of oxidative stress to various medical disorders, such as cancer and ischemic heart disease. Atopic dermatitis (AD) and psoriasis vulgaris (PV) are chronic inflammatory dermatoses, and oxidative stress is presumed to be involved in the development of these diseases as well as their becoming refractory.MethodsIn this study, we collected urine samples from 40 AD patients, 25 PV patients, and 39 healthy volunteers (HVs). The level of 8‐hydroxy‐2′‐deoxyguanosine (8‐OHdG) in each urine sample was measured and then compared among the groups. We also evaluated the relationship between the AD and PV severity and the urinary 8‐OHdG level.ResultsWe found that the urinary 8‐OHdG level was significantly higher in the AD and PV groups than in the HV group. We did not, however, identify an association between the 8‐OHdG level and the age, disease duration, or disease severity in the AD or PV groups.ConclusionsOur results demonstrated an association between urinary 8‐OHdG and the development of AD and PV, suggesting that both are oxidative stress‐related diseases. Treatments or lifestyle improvements focused on anti‐oxidation may therefore be vital for preventing these chronic dermatoses from developing and becoming refractory.
The role of end‐diastolic myocardial fibre stretch on infarct extension
Authors: Leong Chin Neng , Dokos Socrates , Andriyana Andri , Liew Yih Miin , Chan Bee Ting , Abdul Aziz Yang Faridah , Chee Kok‐Han , Sridhar Ganiga Srinivasaiah , Lim Einly 
Published: December 2019

AbstractMyocardial infarct extension, a process involving the enlargement of infarct and border zone, leads to progressive degeneration of left ventricular (LV) function and eventually gives rise to heart failure. Despite carrying a high risk, the causation of infarct extension is still a subject of much speculation. In this study, patient‐specific LV models were developed to investigate the correlation between infarct extension and impaired regional mechanics. Subsequently, sensitivity analysis was performed to examine the causal factors responsible for the impaired regional mechanics observed in regions surrounding the infarct and border zone. From our simulations, fibre strain, fibre stress and fibre stress‐strain loop (FSSL) were the key biomechanical variables affected in these regions. Among these variables, only FSSL was correlated with infarct extension, as reflected in its work density dissipation (WDD) index value, with high WDD indices recorded at regions with infarct extension. Impaired FSSL is caused by inadequate contraction force generation during the isovolumic contraction and ejection phases. Our further analysis revealed that the inadequacy in contraction force generation is not necessarily due to impaired myocardial intrinsic contractility, but at least in part, due to inadequate muscle fibre stretch at end‐diastole, which depresses the ability of myocardium to generate adequate contraction force in the subsequent systole (according to the Frank‐Starling law). Moreover, an excessively stiff infarct may cause its neighbouring myocardium to be understretched at end‐diastole, subsequently depressing the systolic contractile force of the neighbouring myocardium, which was found to be correlated with infarct extension.
Mechanistic Investigation of a Hybrid Zn/V2O5 Rechargeable Battery with a Binary Li+/Zn2+ Aqueous Electrolyte
Authors: Batyrbekuly Dauren , Cajoly Sabrina , Laïk Barbara , Pereira‐Ramos Jean‐Pierre , Emery Nicolas , Bakenov Zhumabay , Baddour‐Hadjean Rita 
Published: December 2019

AbstractLow‐cost, easily processable, and environmentally friendly rechargeable aqueous zinc batteries have great potential for large‐scale energy storage, which justifies their receiving extensive attention in recent years. An original concept based on the use of a binary Li+/Zn2+ aqueous electrolyte is described herein for the case of the Zn/V2O5 system. In this hybrid, the positive side involves mainly the Li+ insertion/deinsertion reaction of V2O5, whereas the negative electrode operates according to zinc dissolution–deposition cycles. The Zn//3 mol L−1 Li2SO4–4 mol L−1 ZnSO4///V2O5 cell worked in the narrow voltage range of 1.6–0.8 V with capacities of approximately 136–125 mA h g−1 at rates of C/20–C/5, respectively. At 1 C, the capacity of 80 mA h g−1 was outstandingly stable for more than 300 cycles with a capacity retention of 100 %. A detailed structural study by XRD and Raman spectroscopy allowed the peculiar response of the V2O5 layered host lattice on discharge–charge and cycling to be unraveled. Strong similarities with the well‐known structural changes reported in nonaqueous lithiated electrolytes were highlighted, although the emergence of the usual distorted δ‐LiV2O5 phase was not detected on discharge to 0.8 V. The pristine host structure was restored and maintained during cycling with mitigated structural changes leading to high capacity retention. The present electrochemical and structural findings reveal a reaction mechanism mainly based on Li+ intercalation, but co‐intercalation of a few Zn2+ ions between the oxide layers cannot be completely dismissed. The presence of zinc cations between the oxide layers is thought to relieve the structural stress induced in V2O5 under operation, and this resulted in a limited volume expansion of 4 %. This fundamental investigation of a reaction mechanism operating in an environmentally friendly aqueous medium has not been reported before.
Flow control with plasma‐based actuator in different velocity regimes: Momentum and heat transfer effects
Authors: Mehdipoor Mostafa , Sohbatzadeh Farshad , Zabihpoor Mahmood 
Published: December 2019

AbstractIn this study, control of the airflow by the direct current (DC) electrical discharge with bare electrodes has been investigated in different velocity regimes. The discharge characteristics of the plasma model are obtained numerically. An induced electrohydrodynamic (EHD) force on neutral flow was characterized based on momentum transfer from charged particles. The change in the incident flow parameters was studied by applying Navier–Stokes (N‐S) equations, considering source terms arising from a weakly ionized plasma. The effect of the discharge on the low‐ and high‐speed flow was simulated in this study. It was concluded that the changes of the velocity profile, airflow pressure, and oblique shock wave could be attributed to the EHD force from a nonthermal plasma to the incoming airflow. It was seen that the incident airflow is accelerated also by the induced EHD force. Our results show that the most important mechanism in the plasma‐based flow control is the momentum transfer from the electrical discharge to the incident flow and that the gas heating has no significant role.
Simultaneous measurement of perillyl alcohol and its metabolite perillic acid in plasma and lung after inhalational administration in Wistar rats
Authors: de Lima Daniela Carneiro , Rodrigues Silvana Vianna , Boaventura Gilson Teles , Cho Hee‐Yeon , Chen Thomas C. , Schönthal Axel H. , Da Fonseca Clovis Orlando 
Published: December 2019

AbstractThe inhalational administration of drugs is a practical and non‐invasive approach with the potential to reduce side effects and with a quick onset of therapeutic activity. Perillyl alcohol (POH) is a monoterpene with antitumor activity that currently is undergoing clinical evaluation as an inhalational anticancer agent. A detection method was developed that will be applicable to pharmacokinetic studies of not only POH, but also its longer‐lived main metabolite, perillic acid (PA), in lung tissue and plasma after inhalational delivery. The anticancer activity of POH was investigated in vitro with the use of various lung cancer cell lines. Toxicity was established by a standard MTT assay, and apoptosis markers were analyzed by Western blot. For the detection of POH and PA in lungs and plasma, albino Wistar rats were used that were exposed to POH inhalation. Tissues were subjected to chromatographic separation on an Agilent Zorbax Eclipse XDB C18 column, followed by detection of absorption in the ultraviolet (UV) range. In vitro, POH exerted cytotoxic activity against six different lung tumor cell lines, and apoptotic cell death was indicated by induction of active caspase 3 and cleavage of poly (ADP‐ribose) polymerase 1 (PARP1). These results demonstrate that inhalational delivery of POH results in effective biodistribution and metabolism of POH in the systemic circulation. In addition, our study introduces a simple, rapid HPLC‐UV method with high accuracy for simultaneous detection of POH and its metabolite PA in plasma, and for sensitive detection of PA in lung tissue, which should prove useful for applications in clinical studies.
Less invasive ventricular reconstruction for ischaemic heart failure
Authors: Klein Patrick , Anker Stefan D. , Wechsler Andrew , Skalsky Ivo , Neuzil Petr , Annest Lon S. , Bifi Mauro , McDonagh Theresa , Frerker Christian , Schmidt Tobias , Sievert Horst , Demaria Anthony N. , Kelle Sebastian 
Published: December 2019

ABSTRACTAimsSurgical ventricular reconstruction to remodel, reshape, and reduce ventricular volume is an effective therapy in selected patients with chronic heart failure (HF) of ischaemic aetiology. The BioVentrix Revivent TC System offers efficacy comparable to conventional surgical ventricular reconstruction and is less invasive utilizing micro‐anchor pairs to exclude scarred myocardium on the beating heart. Here, we present 12‐months follow‐up data of an international multicenter study.Methods and resultsPatients were considered eligible for the procedure when they presented with symptomatic HF [New York Heart Association (NYHA) class ≥II], left ventricular (LV) dilatation and dysfunction caused by myocardial infarction, and akinetic and/or dyskinetic transmural scarred myocardium located in the anteroseptal, anterolateral, and/or apical regions. A total of 89 patients were enrolled and 86 patients were successfully treated (97%). At 12 months, a significant improvement in LV ejection fraction (29 ± 8% vs. 34 ± 9%, P < 0.005) and a reduction of LV volumes was observed (LV end‐systolic and end‐diastolic volume index both decreased: 74 ± 28 mL/m2 vs. 54 ± 23 mL/m2, P < 0.001; and 106 ± 33 mL/m2 vs. 80 ± 26 mL/m2, respectively, P < 0.0001). Four patients (4.5%) died in hospital and survival at 12 months was 90.6%. At baseline, 59% of HF patients were in NYHA class III compared with 22% at 12‐month follow‐up. Improvements in quality of life measures (Minnesota Living with Heart Failure Questionnaire 39 vs. 26 points, P < 0.001) and 6‐min walking test distance (363 m vs. 416 m, P = <0.001) were also significant.ConclusionsTreatment with the Revivent TC System in patients with symptomatic HF results in significant and sustained reduction of LV volumes and improvement of LV function, symptoms, and quality of life.
Clinical value of pre‐discharge bio‐adrenomedullin as a marker of residual congestion and high risk of heart failure hospital readmission
Authors: Pandhi Paloma , ter Maaten Jozine M. , Emmens Johanna E. , Struck Joachim , Bergmann Andreas , Cleland John G. , Givertz Michael M. , Metra Marco , O'Connor Christopher M. , Teerlink John R. , Ponikowski Piotr , Cotter Gad , Davison Beth , van Veldhuisen Dirk J. , Voors Adriaan A. 
Published: December 2019

AbstractAimsRecently, bio‐adrenomedullin (bio‐ADM) was proposed as a congestion marker in heart failure (HF). In the present study, we aimed to study whether bio‐ADM levels at discharge from a hospital admission for worsening HF could provide additional information on (residual) congestion status, diuretic dose titration and clinical outcomes.Methods and resultsPlasma bio‐ADM was measured in 1236 acute HF patients in the PROTECT trial at day 7 or discharge. Median discharge bio‐ADM was 33.7 [21.5–61.5] pg/mL. Patients with higher discharge bio‐ADM levels were hospitalised longer, had higher brain natriuretic peptide levels, and poorer diuretic response (all P < 0.001). Bio‐ADM was the strongest predictor of discharge residual congestion (clinical congestion score > 3) (odds ratio 4.35, 95% confidence interval 3.37–5.62; P < 0.001). Oedema at discharge was one of the strongest predictors of discharge bio‐ADM (β = 0.218; P < 0.001). Higher discharge loop diuretic doses were associated with a poorer diuretic response during hospitalisation (β = 0.187; P < 0.001) and higher bio‐ADM levels (β = 0.084; P = 0.020). High discharge bio‐ADM levels combined with higher use of loop diuretics were independently associated with a greater risk of 60‐day HF rehospitalisation (hazard ratio 4.02, 95% confidence interval 2.23–7.26; P < 0.001).ConclusionIn hospitalised HF patients, elevated pre‐discharge bio‐ADM levels were associated with higher discharge loop diuretic doses and reflected residual congestion. Patients with combined higher bio‐ADM levels and higher loop diuretic use at discharge had an increased risk of rehospitalisation. Assessment of discharge bio‐ADM levels may be a readily applicable marker to identify patients with residual congestion at higher risk of early hospital readmission.
Consultation prevalence among children, adolescents and young adults with pain conditions: A description of age‐ and gender differences
Authors: Bondesson E. , Olofsson T. , Caverius U. , Schelin M. E. C. , Jöud A. 
Published: December 2019

AbstractBackgroundPain is a common complaint presented in healthcare, but most epidemiological pain research has focused either on single pain conditions or on the adult population. The aim of this study was to investigate the 2017 consultation prevalence of a wide range of pain conditions in the general population of young people.MethodsWe used the Skåne Healthcare Register, covering prospectively collected data on all healthcare delivered (primary and secondary care) to the population in the region of Skåne, southern Sweden (population 2017 n = 1,344,689). For individuals aged 1–24 in 2017 (n = 373,178), we calculated the consultation prevalence, stratified by sex and age, and the standardised morbidity ratio (SMR) to assess overall healthcare consultation.ResultsA total of 58,981 (15.8%) individuals consulted at least once for any of the predefined pain conditions. Of these, 13.5% (n = 7,996) consulted four or more times for pain. Abdominal pain, joint pain/myalgia, headache and back/neck pain were the most common complaints. Overall, females had higher consultation prevalence than males: 17.6% versus 14.1% (p < .0001). SMR was 1.82 (95% CI = 1.74–1.87) for females with pain and 1.51 (95% CI = 1.42–1.56) for males with pain. Consultation prevalence increased with age, but this pattern varied between sex and pain condition.ConclusionsAmong individuals under the age of 25, a significant proportion consult for pain already in early ages, and they also have high healthcare consultation rates for conditions other than pain. The even higher consultation rates among young females need additional attention, both in the clinic and in research.SignificanceWe present comprehensive 1‐year healthcare consultation prevalence data covering all levels of care. A significant proportion of children, adolescents and young adults consult for different pain conditions at multiple occasions warranting greater clinical awareness.
Role of ocular cytology in vernal keratoconjunctivitis
Authors: Bruschi Gaia , Ghiglioni Daniele G. , Osnaghi Silvia , Rosazza Chiara , Pires Marafon Denise , Landi Massimo , Marchisio Paola G. 
Published: December 2019

AbstractBackgroundChildren with vernal keratoconjunctivitis (VKC) present symptoms that are similar to other ocular allergies, but more pronounced, and are controlled using topical steroids. To avoid excessive and prolonged use of topical steroid eye drops, over the past 20 years galenic eye drops of cyclosporine with a concentration of 1% to 2% and tacrolimus with a concentration of 0.1% have been introduced as a treatment for the severe and unresponsive forms. The main symptoms of VKC occur most frequently during the spring and tend to get worse during the summer, meaning that affected children tend to avoid exposure to sunlight. The aim of this study was to assess the most common cell types present in the conjunctiva of children with VKC, how ocular treatment can influence them, and whether affected children express a typical conjunctival pattern, which could be useful as a pathognomonic pattern of VKC, allowing us to study this rare eye disease.MethodThis was a cohort study of 56 children, of whom 17 were not receiving any treatment at the time of testing, 14 were using steroid eye drops or had taken them in the previous 10 days, and 25 were treated with cyclosporine eye drops or tacrolimus eye drops 0.1%.ResultChildren in group 1 (no topical therapy) express more epithelial cells, neutrophils, mast cells, eosinophils, and lymphocytes than the other two groups.ConclusionGiven the ease of performance, when conducting further longitudinal studies, the conjunctival cytology examination could be used, on the one hand, to diagnose VKC, especially when the clinical diagnosis is uncertain, and, on the other, to follow disease evolution and monitor the response to topical treatment.
Identifying rate limitation and a guide to design of fast‐charging Li‐ion battery
Authors: Zhang Sheng S. 
Published: December 2019

AbstractFast‐charging is highly demanded for applications requiring short charging time. However, fast‐charging triggers serious problems, leading to decline in charge acceptance and energy efficiency, accelerated capacity degradation, and safety risk. In this work, a three‐electrode coin cell with a Li metal reference electrode is designed to individually record the potential of two electrodes, and measure the impedance of each electrode by using a power‐optimized graphite‐LiNi0.80Co0.15Al0.05O2 electrode couple. It is shown that regardless of the state‐of‐charge the Li‐ion cell's impedance is contributed predominantly by the cathode, and that the cathode's impedance is dominated by the charge‐transfer resistance. In consistence with the impedance results, polarization of the Li‐ion cell is dominated by the cathode. It is surprised to find that no Li plating occurs on the graphite anode even if the charging rate is increased to 10 C (1 C = 1.30 mA cm−2). The results of this work indicate that low overall impedance with a high cathode‐to‐anode impedance ratio is the key to enabling safe fast‐charging, and that fast‐charging Li‐ion batteries without Li plating on the graphite anode is possible if the cathode and graphite anode are optimistically engineered.image
Percolation‐Based Metrics to Quantify the Resilience of Communication Systems
Authors: Benipayo Caesar S. 
Published: December 2019

AbstractResilience theory plays a critical part in the practical design of modern engineered resilient systems that comprise of cyber‐physical systems and components having interdependencies intended to make the whole system robust. In this paper, the percolation‐based metric is utilized to quantify the communication systems' resilience. Because there is no consistent and reliable way to measure resilience, the percolation‐based metric framework is developed to model the component or system failure by removing fractions of the nodes or links, causing the network to transition from functional to non‐functional. The efficacy of the percolation‐based metric model is demonstrated by using the power grid and maritime platform systems (legacy and future design versions) as case studies. The results from the analysis of the maritime communication and power grid systems are presented. The results indicate that percolation‐based metric may be a useful approach for system engineers and designers to gain improved insight into the quantification of communication system resilience.
Approaching The Indian Bullet Train Project Through System Thinking
Authors: Tattikota V Jagadeeswar Rao , Alukal Paul , Nayal Kirti , Das Seema , Rajput Dinesh , Mukundan R 
Published: December 2019

AbstractWe analyze a complex techno‐economic project of the Indian Bullet Train system, under construction via the High‐speed Rail lines in India, with an estimated speed of the train in 350 kmph and covering a distance of 500Km, the project is in collaboration with Japan and estimated cost is to be at Rs.1080 Billions. The groundwork for the Bullet Train Project started in December 2017 and the project dead line is set as August 2023 with land acquisition dead line to be completed by December 2018. The land requirement is 1400 Hectares which is divided into 7000 plots in 195 villages in Gujarat and 104 villages in Maharashtra.Despite location of bullet train project being in India's most economically and industrially developed states, the land acquisition got delayed. In this paper, we develop a systemigram to depict the whole problem. Based on the data available, CLD (Causal loop diagram) is created indicating cause and effect relation and feedback loops. On the application of system thinking approach, this study can be further explored for identifying archetypes.It was identified that the land acquisition process is more influenced by the coordination and interaction between policy makers and locals. It can be said that, if these two are on the same page with regards to the various terms and conditions such as conditions of compensation, then land acquisition will be done on time.