bluuebunny/crossref_metadata_2025_split huggingface dataset:
Interspecies radiative transition in warm and superdense plasma mixtures
Authors: Hu S. X. , Karasiev V. V. , Recoules V. , Nilson P. M. , Brouwer N. , Torrent M. 
Published: April 2020

AbstractSuperdense plasmas widely exist in planetary interiors and astrophysical objects such as brown-dwarf cores and white dwarfs. How atoms behave under such extreme-density conditions is not yet well understood, even in single-species plasmas. Here, we apply thermal density functional theory to investigate the radiation spectra of superdense iron–zinc plasma mixtures at mass densities of ρ = 250 to 2000 g cm−3 and temperatures of kT = 50 to 100 eV, accessible by double-shell–target implosions. Our ab initio calculations reveal two extreme atomic-physics phenomena—firstly, an interspecies radiative transition; and, secondly, the breaking down of the dipole-selection rule for radiative transitions in isolated atoms. Our first-principles calculations predict that for superdense plasma mixtures, both interatomic radiative transitions and dipole-forbidden transitions can become comparable to the normal intra-atomic Kα-emission signal. These physics phenomena were not previously considered in detail for extreme high-density plasma mixtures at super-high energy densities.
Confining H3PO4 network in covalent organic frameworks enables proton super flow
Authors: Tao Shanshan , Zhai Lipeng , Dinga Wonanke A. D. , Addicoat Matthew A. , Jiang Qiuhong , Jiang Donglin 
Published: April 2020

AbstractDevelopment of porous materials combining stability and high performance has remained a challenge. This is particularly true for proton-transporting materials essential for applications in sensing, catalysis and energy conversion and storage. Here we report the topology guided synthesis of an imine-bonded (C=N) dually stable covalent organic framework to construct dense yet aligned one-dimensional nanochannels, in which the linkers induce hyperconjugation and inductive effects to stabilize the pore structure and the nitrogen sites on pore walls confine and stabilize the H3PO4 network in the channels via hydrogen-bonding interactions. The resulting materials enable proton super flow to enhance rates by 2–8 orders of magnitude compared to other analogues. Temperature profile and molecular dynamics reveal proton hopping at low activation and reorganization energies with greatly enhanced mobility.
In-situ preservation of nitrogen-bearing organics in Noachian Martian carbonates
Authors: Koike Mizuho , Nakada Ryoichi , Kajitani Iori , Usui Tomohiro , Tamenori Yusuke , Sugahara Haruna , Kobayashi Atsuko 
Published: April 2020

AbstractUnderstanding the origin of organic material on Mars is a major issue in modern planetary science. Recent robotic exploration of Martian sedimentary rocks and laboratory analyses of Martian meteorites have both reported plausible indigenous organic components. However, little is known about their origin, evolution, and preservation. Here we report that 4-billion-year-old (Ga) carbonates in Martian meteorite, Allan Hills 84001, preserve indigenous nitrogen(N)-bearing organics by developing a new technique for high-spatial resolution in situ N-chemical speciation. The organic materials were synthesized locally and/or delivered meteoritically on Mars during Noachian age. The carbonates, alteration minerals from the Martian near-surface aqueous fluid, trapped and kept the organic materials intact over long geological times. This presence of N-bearing compounds requires abiotic or possibly biotic N-fixation and ammonia storage, suggesting that early Mars had a less oxidizing environment than today.
Non-coding somatic mutations converge on the PAX8 pathway in ovarian cancer
Authors: Corona Rosario I. , Seo Ji-Heui , Lin Xianzhi , Hazelett Dennis J. , Reddy Jessica , Fonseca Marcos A. S. , Abassi Forough , Lin Yvonne G. , Mhawech-Fauceglia Paulette Y. , Shah Sohrab P. , Huntsman David G. , Gusev Alexander , Karlan Beth Y. , Berman Benjamin P. , Freedman Matthew L. , Gayther Simon A. , Lawrenson Kate 
Published: April 2020

AbstractThe functional consequences of somatic non-coding mutations in ovarian cancer (OC) are unknown. To identify regulatory elements (RE) and genes perturbed by acquired non-coding variants, here we establish epigenomic and transcriptomic landscapes of primary OCs using H3K27ac ChIP-seq and RNA-seq, and then integrate these with whole genome sequencing data from 232 OCs. We identify 25 frequently mutated regulatory elements, including an enhancer at 6p22.1 which associates with differential expression of ZSCAN16 (P = 6.6 × 10-4) and ZSCAN12 (P = 0.02). CRISPR/Cas9 knockout of this enhancer induces downregulation of both genes. Globally, there is an enrichment of single nucleotide variants in active binding sites for TEAD4 (P = 6 × 10-11) and its binding partner PAX8 (P = 2×10-10), a known lineage-specific transcription factor in OC. In addition, the collection ofcisREs associated with PAX8 comprise the most frequently mutated set of enhancers in OC (P = 0.003). These data indicate that non-coding somatic mutations disrupt the PAX8 transcriptional network during OC development.
Macroscopic Polarization Change via Electron Transfer in a Valence Tautomeric Cobalt Complex
Authors: Wu Shu-Qi , Liu Meijiao , Gao Kaige , Kanegawa Shinji , Horie Yusuke , Aoyama Genki , Okajima Hajime , Sakamoto Akira , Baker Michael L. , Huzan Myron S. , Bencok Peter , Abe Tsukasa , Shiota Yoshihito , Yoshizawa Kazunari , Xu Wenhuang , Kou Hui-Zhong , Sato Osamu 
Published: April 2020

AbstractPolarization change induced by directional electron transfer attracts considerable attention owing to its fast switching rate and potential light control. Here, we investigate electronic pyroelectricity in the crystal of a mononuclear complex, [Co(phendiox)(rac-cth)](ClO4)·0.5EtOH (1·0.5EtOH, H2phendiox = 9, 10-dihydroxyphenanthrene,rac-cth = racemic 5, 5, 7, 12, 12, 14-hexamethyl-1, 4, 8, 11-tetraazacyclotetradecane), which undergoes a two-step valence tautomerism (VT). Correspondingly, pyroelectric current exhibits double peaks in the same temperature domain with the polarization change consistent with the change in dipole moments during the VT process. Time-resolved Infrared (IR) spectroscopy shows that the photo-induced metastable state can be generated within 150 ps at 190 K. Such state can be trapped for tens of minutes at 7 K, showing that photo-induced polarization change can be realized in this system. These results directly demonstrate that a change in the molecular dipole moments induced by intramolecular electron transfer can introduce a macroscopic polarization change in VT compounds.
MethCORR modelling of methylomes from formalin-fixed paraffin-embedded tissue enables characterization and prognostication of colorectal cancer
Authors: Mattesen Trine B. , Rasmussen Mads H. , Sandoval Juan , Ongen Halit , Árnadóttir Sigrid S. , Gladov Josephine , Martinez-Cardus Anna , Castro de Moura Manuel , Madsen Anders H. , Laurberg Søren , Dermitzakis Emmanouil T. , Esteller Manel , Andersen Claus L. , Bramsen Jesper B. 
Published: April 2020

Abstract
Transcriptional characterization and classification has potential to resolve the inter-tumor heterogeneity of colorectal cancer and improve patient management. Yet, robust transcriptional profiling is difficult using formalin-fixed, paraffin-embedded (FFPE) samples, which complicates testing in clinical and archival material. We present MethCORR, an approach that allows uniform molecular characterization and classification of fresh-frozen and FFPE samples. MethCORR identifies genome-wide correlations between RNA expression and DNA methylation in fresh-frozen samples. This information is used to infer gene expression information in FFPE samples from their methylation profiles. MethCORR is here applied to methylation profiles from 877 fresh-frozen/FFPE samples and comparative analysis identifies the same two subtypes in four independent cohorts. Furthermore, subtype-specific prognostic biomarkers that better predicts relapse-free survival (HR = 2.66, 95%CI [1.67–4.22], P value < 0.001 (log-rank test)) than UICC tumor, node, metastasis (TNM) staging and microsatellite instability status are identified and validated using DNA methylation-specific PCR. The MethCORR approach is general, and may be similarly successful for other cancer types.
A masked initiation region in retinoblastoma protein regulates its proteasomal degradation
Authors: Tomita Takuya , Huibregtse Jon M. , Matouschek Andreas 
Published: April 2020

AbstractRetinoblastoma protein (Rb) is a tumor suppressor that binds and represses E2F transcription factors. In cervical cancer cells, human papilloma virus (HPV) protein E7 binds to Rb, releasing it from E2F to promote cell cycle progression, and inducing ubiquitination of Rb. E7-mediated proteasomal degradation of Rb requires action by another protease, calpain, which cleaves Rb after Lys 810. However, it is not clear why cleavage is required for Rb degradation. Here, we report that the proteasome cannot initiate degradation efficiently on full-length Rb. Calpain cleavage exposes a region that is recognized by the proteasome, leading to rapid proteolysis of Rb. These findings identify a mechanism for regulating protein stability by controlling initiation and provide a better understanding of the molecular mechanism underlying transformation by HPV.
Vertical two-dimensional layered fused aromatic ladder structure
Authors: Noh Hyuk-Jun , Im Yoon-Kwang , Yu Soo-Young , Seo Jeong-Min , Mahmood Javeed , Yildirim Taner , Baek Jong-Beom 
Published: April 2020

AbstractPlanar two-dimensional (2D) layered materials such as graphene, metal-organic frameworks, and covalent-organic frameworks are attracting enormous interest in the scientific community because of their unique properties and potential applications. One common feature of these materials is that their building blocks (monomers) are flat and lie in planar 2D structures, with interlayer π–π stacking, parallel to the stacking direction. Due to layer-to-layer confinement, their segmental motion is very restricted, which affects their sorption/desorption kinetics when used as sorbent materials. Here, to minimize this confinement, a vertical 2D layered material was designed and synthesized, with a robust fused aromatic ladder (FAL) structure. Because of its unique structural nature, the vertical 2D layered FAL structure has excellent gas uptake performance under both low and high pressures, and also a high iodine (I2) uptake capacity with unusually fast kinetics, the fastest among reported porous organic materials to date.
On the importance of time-resolved electrochemical evaluation in corrosion inhibitor-screening studies
Authors: Taheri Peyman , Milošev Ingrid , Meeusen Mats , Kapun Barbara , White Paul , Kokalj Anton , Mol Arjan 
Published: April 2020

AbstractEfficiency of corrosion inhibitors in aqueous solutions depends on several interfacial parameters, which may vary over time. Therefore, reliable electrochemical techniques are demanded for screening the efficiency of corrosion inhibitors and monitoring their performance over time. Here, we evaluate corrosion inhibition efficiency of imidazole-based compounds on bare Cu surfaces and highlight the importance of electrochemical evaluation of the inhibitor over time, characterized by linear polarization resistance techniques as a reliable, instantaneous and non-invasive method for assessing intrinsic inhibitor performance in lab screening studies.
Relationship between quality of life and behavioural disorders in children with persistent asthma: a Multiple Indicators Multiple Causes (MIMIC) model
Authors: Montalbano Laura , Ferrante Giuliana , Montella Silvia , Cilluffo Giovanna , Di Marco Antonio , Bozzetto Sara , Di Palmo Emanuela , Licari Amelia , Leonardi Lucia , Caldarelli Valeria , Ghezzi Michele , La Grutta Stefania , Rusconi Franca ,   the Italian Pediatric Severe Asthma Network (IPSAN) Program of Italian Paediatric Respiratory Society (IPRS), Amarri S. , Barni S. , Capizzi A. , Cardinale F. , Carraro S. , Cazzato S. , Cutrera R. , Di Pillo S. , Duse M. , Fenu G. , Kantar A. , Leonardi S. , Lombardi E. , Marseglia G. L. , Nosetti L. , Novembre E. , Patria M. F. , Piacentini G. , Pisi G. , Ricci G. , Sacco O. , Santamaria F. , Tenero L. , Tosca M. A. , Tripodi M. C. , Volpini A. 
Published: April 2020

AbstractKnowledge on multiple interdependences between quality of life (QoL) and behavioural problems in relation to asthma severity and control is undetermined. The aims of the study were: (i) to assess the relationship of QoL and behavioural problems with asthma severity and control (ii) to predict children’s “abnormal/borderline” status with variation in QoL. For these purposes a multicenter case-control study on 47 Severe Asthma (SA) and 94 Moderate Asthma (MA) children was performed. The MIMIC approach was applied to investigate the effect of SA and non-controlled asthma (NC) on QoL and behavioural disorders. Logistic regression was used to estimate probabilities of having an “abnormal/borderline” status with variation in QoL. The MIMIC model showed that the magnitude of the effect of SA and NC was larger on QoL (β = −0.37 and β = −0.30, respectively) than on behavioural problems (β = 0.27). With regards to the probability of having a borderline status, in MA a QoL of 1 returned a probability of 0.81, whereas in SA a QoL of 1 returned a probability of 0.89. In conclusion, SA children are highly affected by impaired QoL and behavioural problems. The MIMIC model allowed us to obtain a comprehensive assessment of QoL and behavioural problems with asthma severity and control.
A 96-well culture platform enables longitudinal analyses of engineered human skeletal muscle microtissue strength
Authors: Afshar Mohammad E. , Abraha Haben Y. , Bakooshli Mohsen A. , Davoudi Sadegh , Thavandiran Nimalan , Tung Kayee , Ahn Henry , Ginsberg Howard J. , Zandstra Peter W. , Gilbert Penney M. 
Published: April 2020

AbstractThree-dimensional (3D) in vitro models of human skeletal muscle mimic aspects of native tissue structure and function, thereby providing a promising system for disease modeling, drug discovery or pre-clinical validation, and toxicity testing. Widespread adoption of this research approach is hindered by the lack of easy-to-use platforms that are simple to fabricate and that yield arrays of human skeletal muscle micro-tissues (hMMTs) in culture with reproducible physiological responses that can be assayed non-invasively. Here, we describe a design and methods to generate a reusable mold to fabricate a 96-well platform, referred to as MyoTACTIC, that enables bulk production of 3D hMMTs. All 96-wells and all well features are cast in a single step from the reusable mold. Non-invasive calcium transient and contractile force measurements are performed on hMMTs directly in MyoTACTIC, and unbiased force analysis occurs by a custom automated algorithm, allowing for longitudinal studies of function. Characterizations of MyoTACTIC and resulting hMMTs confirms the capability of the device to support formation of hMMTs that recapitulate biological responses. We show that hMMT contractile force mirrors expected responses to compounds shown by others to decrease (dexamethasone, cerivastatin) or increase (IGF-1) skeletal muscle strength. Since MyoTACTIC supports hMMT long-term culture, we evaluated direct influences of pancreatic cancer chemotherapeutics agents on contraction competent human skeletal muscle myotubes. A single application of a clinically relevant dose of Irinotecan decreased hMMT contractile force generation, while clear effects on myotube atrophy were observed histologically only at a higher dose. This suggests an off-target effect that may contribute to cancer associated muscle wasting, and highlights the value of the MyoTACTIC platform to non-invasively predict modulators of human skeletal muscle function.
Spontaneous spin-up induced by turbulence-driven topological transition of orbits in a collisionless tokamak plasma
Authors: Wang Shaojie 
Published: April 2020

AbstractSpontaneous spin-up are widely observed in tokamak plasmas, which is crucially important for plasma confinement. A kinetic theory is proposed to show that a toroidal rotation of core plasma is induced by the topological transition of orbits driven by turbulent diffusion in a collisionless tokamak plasma. The theoretical prediction agrees well with the well-known Rice-scaling of intrinsic core plasma flow. This new theory predicts an intrinsic co-current core parallel flow of ~100 km/s in the International Thermonuclear Experimental Reactor.
Late-pregnancy uterine artery ligation increases susceptibility to postnatal Western diet-induced fat accumulation in adult female offspring
Authors: Jahandideh Forough , Bourque Stephane L. , Armstrong Edward A. , Cherak Stephana J. , Panahi Sareh , Macala Kimberly F. , Davidge Sandra T. , Yager Jerome Y. 
Published: April 2020

AbstractStressors during the fetal and postnatal period affect the growth and developmental trajectories of offspring, causing lasting effects on physiologic regulatory systems. Here, we tested whether reduced uterine artery blood flow in late pregnancy would alter body composition in the offspring, and whether feeding offspring a western diet (WD) would aggravate these programming effects. Pregnant rats underwent bilateral uterine artery ligation (BUAL) or sham surgery on gestational day (GD)18 (term = GD22). At weaning, offspring from each group received either a normal diet (ND) or a WD. BUAL surgery increased fetal loss and caused offspring growth restriction, albeit body weights were no longer different at weaning, suggesting postnatal catch-up growth. BUAL did not affect body weight gain, fat accumulation, or plasma lipid profile in adult male offspring. In contrast, while ND-fed females from BUAL group were smaller and leaner than their sham-littermates, WD consumption resulted in excess weight gain, fat accumulation, and visceral adiposity. Moreover, WD increased plasma triglycerides and cholesterol in the BUAL-treated female offspring without any effect on sham littermates. These results demonstrate that reduced uterine artery blood flow during late pregnancy in rodents can impact body composition in the offspring in a sex-dependent manner, and these effects may be exacerbated by postnatal chronic WD consumption.
Wideband tunable microwave signal generation in a silicon-micro-ring-based optoelectronic oscillator
Authors: Do Phuong T. , Alonso-Ramos Carlos , Le Roux Xavier , Ledoux Isabelle , Journet Bernard , Cassan Eric 
Published: April 2020

AbstractSi photonics has an immense potential for the development of compact and low-loss opto-electronic oscillators (OEO), with applications in radar and wireless communications. However, current Si OEO have shown a limited performance. Si OEO relying on direct conversion of intensity modulated signals into the microwave domain yield a limited tunability. Wider tunability has been shown by indirect phase-modulation to intensity-modulation conversion. However, the reported tuning range is lower than 4 GHz. Here, we propose a new approach enabling Si OEOs with wide tunability and direct intensity-modulation to microwave conversion. The microwave signal is created by the beating between an optical source and single sideband modulation signal, selected by an add-drop ring resonator working as an optical bandpass filter. The tunability is achieved by changing the wavelength spacing between the optical source and a resonance peak of the resonator. Based on this concept, we experimentally demonstrate microwave signal generation between 6 GHz and 18 GHz, the widest range for a Si-micro-ring-based OEO. Moreover, preliminary results indicate that the proposed Si OEO provides precise refractive index monitoring, with a sensitivity of 94350 GHz/RIU and a potential limit of detection of only 10−8 RIU, opening a new route for the implementation of high-performance Si photonic sensors.
Kv1 potassium channels control action potential firing of putative GABAergic deep cerebellar nuclear neurons
Authors: Feria Pliego Jessica Abigail , Pedroarena Christine M. 
Published: April 2020

AbstractLow threshold voltage activated Kv1 potassium channels play key roles in regulating action potential (AP) threshold, neural excitability, and synaptic transmission. Kv1 channels are highly expressed in the cerebellum and mutations of human Kv1 genes are associated to episodic forms of ataxia (EAT-1). Besides the well-established role of Kv1 channels in controlling the cerebellar basket-Purkinje cells synapses, Kv1 channels are expressed by the deep cerebellar nuclear neurons (DCNs) where they regulate the activity of principal DCNs carrying the cerebellar output. DCNs include as well GABAergic neurons serving important functions, such as those forming the inhibitory nucleo-olivary pathway, the nucleo-cortical DCNs providing feed-back inhibition to the cerebellar cortex, and those targeting principal DCNs, but whether their function is regulated by Kv1 channels remains unclear. Here, using cerebellar slices from mature GAD67-GFP mice to identify putative GABAergic-DCNs (GAD + DCN) we show that specific Kv1 channel blockers (dendrotoxin-alpha/I/K, DTXs) hyperpolarized the threshold of somatic action potentials, increased the spontaneous firing rate and hampered evoked high frequency repetitive responses of GAD + DCNs. Moreover, DTXs induced somatic depolarization and tonic firing in previously silent, putative nucleo-cortical DCNs. These results reveal a novel role of Kv1 channels in regulating GABAergic-DCNs activity and thereby, cerebellar function at multiple levels.
Circ-camk4 involved in cerebral ischemia/reperfusion induced neuronal injury
Authors: Zhang Zhao-huan , Wang Yue-rong , Li Fei , Liu Xiu-ling , Zhang Hui , Zhu Zhong-zheng , Huang Hai , Xu Xiao-hui 
Published: April 2020

AbstractStroke and subsequent cerebral ischemia/reperfusion (I/R) injury is a frequently occurring disease that can have serious consequences in the absence of timely intervention. Circular RNAs (circRNAs) in association with microRNAs (miRNAs) and RNA-binding proteins (RBPs) can influence gene expression. However, whether circRNAs have a role in cerebral I/R injury pathogenesis, especially soon after onset, is unclear. In this study, we used the SD rat middle cerebral artery occlusion (MCAO) model of stroke to examine the role of circRNAs in cerebral I/R injury. We used high-throughput sequencing (HTS) to compare the expression levels of circRNAs in cerebral cortex tissue from MCAO rats during the occlusion-reperfusion latency period 3 hours after I/R injury with those in control cerebral cortices. Our sequencing results revealed that expression levels of 44 circRNAs were significantly altered after I/R, with 16 and 28 circRNAs showing significant up- and down-regulation, respectively, relative to levels in control cortex. We extended these results in vitro in primary cultured neuron cells exposed to oxygen-glucose deprivation/reperfusion (OGD/R) using qRT-PCR to show that levels of circ-camk4 were increased in OGD/R neurons relative to control neurons. Bioinformatics analyses predicted that several miRNAs could be associated with circ-camk4 and this prediction was confirmed in a RNA pull-down assay. KEGG analysis to predict pathways that involve circ-camk4 included the glutamatergic synapse pathway, MAPK signaling pathway, and apoptosis signaling pathways, all of which are known to be involved in brain injury after I/R. Our results also demonstrate that levels of the human homolog to circ-camk4 (hsa-circ-camk4) are elevated in SH-SY5Y cells exposed to OGD/R treatment. Overexpression of hsa-circ-camk4 in SH-SY5Y cells significantly increased the rate of cell death after OGD/R, suggesting that circ-camk4 may play a key role in progression of cerebral I/R injury.