bluuebunny/crossref_metadata_2025_split huggingface dataset:
Co-delivery of Doxorubicin and Curcumin with Polypeptide Nanocarrier for Synergistic Lymphoma Therapy
Authors: Guo Wei , Song Yuanyuan , Song Wantong , Liu Yingmin , Liu Zhihe , Zhang Dawei , Tang Zhaohui , Bai Ou 
Published: May 2020

AbstractThe traditional chemotherapy, including Adriamycin (Doxorubicin, DOX), is widely used and is part of the first-line chemotherapy of invasive B cell lymphoma. DOX is nonselective cytotoxic drug and has many adverse effects, which limit its clinical application in combination with other anti-cancer drugs. Optimization of the delivery system targeting tumor microenvironment could be a feasible approach that may have significant clinical significance. Further, combination of DOX with other anticancer drugs, such as curcumin, can enhance the synergistic effects, possibly through epigenetic mechanisms. Hence, we evaluated the efficacy and toxicity of novel nanoparticles that enable the co-delivery of DOX and curcumin in the treatment of invasive B cell lymphoma both in vivo and vitro. The polymer nano materials [mPEG-b-P(Glu-co-Phe)] was used to co-load DOX and curcumin (CUR): L-DOX + CUR. DOX signal was measured to determine the ability of the drugs entering the cells by flow cytometry, and the different enrichment areas in the cells were directly observed by confocal microscope. The toxicity of LDOX + CUR was tested by CCK-8 assay in different cells, and the synergistic coefficients were calculated. The cell apoptosis and the possible mechanisms of apoptosis pathways regulation by L-DOX + CUR were examined using flow cytometry and Western Blot. The MTD (maximum tolerable dose) test was performed in mice. Tumor-bearing SCID mice (i.e., BJAB cell) were used to evaluate the in vivo efficacy of L-DOX + CUR. L-DOX + CUR, was prepared successfully, and the mole ratio of DOX and CUR fixed in 1.0:1.2. (DOX loading rate 9.7%, CUR loading rate 8.1%). L-DOX + CUR exhibited increased intracellular delivery and the main enrichment area of DOX was nucleus. L-DOX + CUR increased cytotoxicity, induced higher rates of apoptosis, and had synergistic effect, especially in BJAB cells (min CI 0.019). It even had epigenetic effect and affected miRNA levels favorably by down-regulating miR-21, miR-199a and up-regulating miR-98 and miR-200c. Additionally, L-DOX + CUR increased MTD in Kunming mice (i.e., 25 mg/kg), compared to DOX (10 mg/kg) and L-DOX (20 mg/kg). In BJAB cell bearing SCID mice, L-DOX + CUR treatment suppressed tumor growth compared to DOX or L-DOX alone, and exhibited less weight loss in mice. We developed new polymer nanoparticles-mPEG-b-P (Glu-co-Phe) co-loaded with DOX and DUR. L-DOX + CUR exhibited synergistic cytotoxic and apoptotic effects on invasive B cell lymphoma. Treatment of L-DOX + CUR potentiated tumor killing in xenografts and reduced toxicity in vivo.
Synergistic therapeutic efficacy of ebselen and silver ions against multidrug-resistant Acinetobacter baumannii-induced urinary tract infections
Authors: Dong Chuanjiang , Wang Jun , Chen Huan , Wang Peng , Zhou Jingxuan , Zhao Ying , Zou Lili 
Published: May 2020

Abstract
Ebselen (EbSe), an organo-selenium compound with well-characterized toxicology and pharmacology, exhibited potent antibacterial activity against glutathione (GSH)-positive bacteria when combined with silver ions (Ag+). In this paper, the strong bactericidal activity of EbSe–Ag+ against multidrug-resistant (MDR) Acinetobacter baumannii has been confirmed, and its efficacy was mainly based on the inhibition of thioredoxin reductase (TrxR) activity and the depletion of the total GSH amount. Moreover, the therapeutic effect of EbSe–Ag+ on urinary tract infection was assessed in a mouse model induced with A. baumannii 0361# strain. The treatment with EbSe–Ag+ significantly reduced the bacterial load and expression levels of the pro-inflammatory cytokines interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ) in bladder lesions; meanwhile, the pathological experiment showed that A. baumannii-induced changes in EbSe–Ag+ treated mice were much attenuated than that in the control group. Thus, all the results obtained here may lay the foundation for further analysis and development of EbSe–Ag+ as potential antibacterial agents for MDR A. baumannii-induced urinary tract infection treatment.
Raman spectrum feature extraction and diagnosis of oil–paper insulation ageing based on kernel principal component analysis
Authors: Yang Dingkun , Weigen Chen , Haiyang Shi , Fu Wan , Yongkuo Zhou 
Published: May 2020

AbstractRaman spectroscopy, with its specific ability to generate a unique fingerprint‐like spectrum of certain substances, has attracted much attention in diagnosing the ageing degree of oil–paper insulation. In this study, the feature extraction and ageing diagnosis methods of oil–paper insulation Raman spectroscopy data are further studied. Based on the non‐linear analysis of Raman spectra of different ageing samples, kernel principal component analysis was applied to extract the spectral features, and the back‐propagation neural network was used to build a diagnosis model with high diagnostic accuracy. The results show that Raman spectroscopy combined with kernel principal component analysis and the back‐propagation neural network can diagnose the ageing state of oil–paper insulation, with a diagnostic accuracy of 91.43% (64/70). The proposed method provides an effective and feasible method for the ageing assessment of oil‐immersed electrical equipment.
Kinesiotaping Diminishes Delayed Muscle Soreness but does not Improve Muscular Performance
Authors: Camacho Maria Alejandra , Herrera Esperanza , Barela Jose Angelo , Delgado-Diaz Diana Carolina 
Published: May 2020

AbstractThis study aimed at determining the effect of kinesio-taping (KT) on muscle performance and delayed onset muscle soreness (DOMS) after exercise induced muscle damaged. Sixty-six healthy men volunteered to participate (age:18–25 y/o), who performed 200 isokinetic lengthening contractions of the dominant quadriceps. Then subjects were randomized to either control (no treatment), sham (no tape tension), or KT (10% tape tension) groups. Muscle performance was assessed by peak torque and muscular work during maximal isometric and concentric isokinetic contractions. DOMS intensity was assessed using a visual analog scale. Measurements were taken pre-exercise (Pre), 48 h and 96 h post-exercise. Repeated measures ANOVA was used for comparisons within group, and ANCOVA for comparisons among groups. Muscle damage was confirmed in all participants by an increase in CK activity level (p<0.01). Decrease in isometric and isokinetic peak torque was detected at 48 h in the control and sham groups (p<0.01). Muscular work decreased in all groups at 48 h (p<0.01). No differences between groups were detected in muscular performance variables. Increase in DOMS intensity was determined in all groups at 48 h. Comparisons between groups showed lower DOMS intensity in the KT group at 48 h. KT decreased DOMS intensity perception after exercise-induced muscle damage; however, it did not impact muscular performance.
Evolution of Functional Recovery using Hop Test Assessment after ACL Reconstruction
Authors: Rambaud Alexandre J.M. , Rossi Jérémy , Neri Thomas , Samozino Pierre , Edouard Pascal 
Published: May 2020

AbstractThe purpose of this study was to evaluate improvements in functional performance through the use of the Limb Symmetry Index of Single and Triple Hop tests between 12 and 52 weeks after anterior cruciate ligament reconstruction, and to compare these values with usual time-based and performance-based criteria used during the return to sport continuum. Repeated functional assessments using Single and Triple Hop Tests at 12, 16, 22, 26, 39 and 52 postoperative weeks were evaluated. At each session, the median and interquartile range of Limb Symmetry Index of tests were calculated and compared with the usual criteria: return to participation:≥85%, between 12–16 w; return to play:≥90%, between 26–39 w. The results indicate that the median increased over time to 39 postoperative weeks and then stabilized. For Single Hop Test, wide variability was seen at 12 and 16 weeks (interquartile range=20%); this was lower from 22 to 52 weeks (interquartile range=8–6%). At 12 weeks for Single Hop Test, the median was 83.6% and did not meet>85% criteria for return to participation. Hop tests could be interesting functional tests to follow the functional recovery and help decision-making regarding return to participation and return to play.
Low-Field Magnetic Resonance Imaging
Authors: Klein Hans-Martin 
Published: May 2020

Background For more than two decades, the focus of technological progress in MRI was restricted to systems with a field strength of 1.5 T and higher. Low- and mid-field MRI systems, which offer some specific advantages, are vanishing from the market. This article is intended to initiate a re-evaluation of the factor ‘field strength’ in MR imaging.
Method Literature review was carried out using MEDLINE database (via Pubmed) over a time span from 1980 to 2019 using free-text and Medical Subject headings (MeSH). Article selection was based on relevance and evidence.
Results and Conclusion Low-field MR systems are meanwhile rare in clinical imaging. MRI systems with a lower field strength provide a reduced signal-noise ratio (SNR) and spectral differentiation. However, these systems offer a variety of advantages: Shorter T1 relaxation, better T1 contrast, fewer metal artifacts, reduced susceptibility and chemical shift artifacts, fewer dielectric effects, better tissue penetration, less RF-power deposition, fewer ‘missile effects’, reduced effect on biomedical implants such as shunt valves, less energy and helium consumption. If we free ourselves from the constraints of high-field strength, we are able to offer multiple medical, economic and ecologic advantages to our patients. The development of high-quality low-field MRI is possible and necessary.
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Periodontal Ligament Cell Sheets and RGD-Modified Chitosan Improved Regeneration in the Horizontal Periodontal Defect Model
Authors: Amir Lisa R. , Soeroso Yuniarti , Fatma Dewi , Sunarto Hari , Sulijaya Benso , Idrus Erik , Rahdewati Herlis , Tjokrovonco Angelia M. , Izumi Kenji , Abbas Basril , Latief Fourier D. E. 
Published: May 2020

Abstract
Objective The aim of this study was to examine the potential of periodontal ligament (PDL) cells sheet and arginine-glycyl-aspartic acid (RGD)-modified chitosan scaffold for periodontal tissue regeneration in horizontal periodontal defect model.
Materials and Methods PDL cell cytotoxicity was tested with 3–[4,5- dimethylthiazol-2yl]–2,5-diphenyl-2H-tetrazolium bromide assay. Cell migration toward the chitosan-based materials was analyzed with trans-well migration assay. Horizontal periodontal defect model was created in four maxillary and mandibular lateral incisors of Macaque nemestrina. Following periodontal therapy, the sites were transplanted with various regenerative materials: (1) chitosan, (2) RGD-modified chitosan, (3) PDL cell sheet with chitosan, (4) PDL cell sheet with RGD-modified chitosan. The periodontal tissue regeneration was evaluated clinically and radiographically. Gingival crevicular fluids were collected each week to evaluate cementum protein-1 (CEMP-1) expression with enzyme-linked immunosorbent assay, while the biopsies were retrieved after 4 weeks for histological and microcomputed tomography evaluation.
Statistical Analysis Data was statistically analyzed using GraphPad Prism 6 for MacOS X. Normality was tested using the Shapiro–Wilk normality test. The Kruskal–Wallis test was used to compare the groups. Significance was accepted when p < 0.05.
Results Clinical examination revealed more epithelial attachment was formed in the group with PDL cell sheet with RGD-modified chitosan. Similarly, digital subtraction radiography analysis showed higher gray scale, an indication of higher alveolar bone density surrounded the transplanted area, as well as higher CEMP-1 protein expression in this group. The incorporation of RGD peptide to chitosan scaffold in the group with or without PDL cells sheet reduced the distance of cement–enamel junction to the alveolar bone crest; hence, more periodontal tissue formed.
Conclusions Horizontal periodontal defect model could be successfully created in M. nemestrina model. Combination of PDL cell sheet and RGD-modified chitosan resulted in the higher potential for periodontal tissue regeneration. The results of this study highlight the PDL cell sheet and RGD-modified chitosan as a promising approach for future clinical use in periodontal regeneration.
Relationship of Peak Expiratory Flow Rate with Waist Circumference, Hip Circumference, and Waist-to-Hip Ratio in Young Adults
Authors: Rai Richa Hirendra , Gupta Shailly , Mohd Asif 
Published: May 2020

Abstract
Introduction It has been observed that respiratory problems and the consequent pulmonary dysfunction are associated with an increase in waist circumference (WC), a measure of obesity. Both comorbidities result in changes in the overall health, quality of life, and life span. The literature shows that body fat distribution can affect pulmonary function. Pulmonary function test (PFT) is a basic and essential tool for the diagnosis and assessment of pulmonary dysfunction, lung diseases, and treatment effects. However, the facilities to measure PFT are not readily available for individual use. Peak expiratory flow rate (PEFR) is a simple and important biomarker that can measure the pulmonary function and can help monitor lung function to initiate early preventive measure. PEFR can be measured by a portable device, which can be carried anytime anywhere. Using this portable instrument, this study was undertaken to investigate whether there is any relationship of PEFR with the biomarkers of obesity, such as WC, hip circumference (HC), and waist-to-hip ratio (WHR) in young adults.
Materials and Methods A total of 84 participants of either sex (38 males and 46 females) aged 20 to 26 years were recruited from a college of South Delhi. Demographic and anthropometric data regarding WC, HC, and WHR were recorded. For PEFR, three consecutive measurements were taken at about 2-minute intervals, and the maximum of the three values was taken into account.
Results Though not statistically significant, but a tendency of negative correlation was observed between PEFR and WC (r = –0.43) and between PEFR and WHR (r = –0.48) in males. However, no significant correlation of PEFR with any of the obesity markers was found in females. No correlation was observed between PEFR and HC in the participants of either sex.
Conclusion PEFR may be adversely affected with increase in WC and WHR, as seen in males. However, no association was observed between PEFR and HC in participants of either sex.
Hepatic gap junctions amplify alcohol liver injury by propagating cGAS-mediated IRF3 activation
Authors: Luther Jay , Khan Sanjoy , Gala Manish K. , Kedrin Dmitry , Sridharan Gautham , Goodman Russell P. , Garber John J. , Masia Ricard , Diagacomo Erik , Adams Daniel , King Kevin R. , Piaker Samuel , Reinecker Hans-Christian , Yarmush Martin L. , Argemi Josepmaria , Bataller Ramon , Dienstag Jules L. , Chung Raymond T. , Patel Suraj J. 
Published: May 2020

Significance
Alcohol-related liver disease is a leading cause of mortality worldwide. Improved patient outcomes will be driven by advances in our understanding of disease pathogenesis, allowing for the development of novel, targeted therapeutics. By combining patient data with animal models of alcohol-related liver disease, we demonstrate the critical importance of the cyclic guanosine monophosphate–adenosine monophosphate synthase-interferon regulatory factor 3 (cGAS-IRF3) pathway and its amplification through hepatocyte intercellular communication in fueling liver injury caused by alcohol. Thus, our results identify two potentially druggable pathways to improve patient outcomes and reduce mortality resulting from this devastating disease.
The Noise Collector for sparse recovery in high dimensions
Authors: Moscoso Miguel , Novikov Alexei , Papanicolaou George , Tsogka Chrysoula 
Published: May 2020

SignificanceThe ability to detect sparse signals from noisy, high-dimensional data is a top priority in modern science and engineering. For optimal results, current approaches need to tune parameters that depend on the level of noise, which is often difficult to estimate. We develop a parameter-free, computationally efficient,ℓ1-norm minimization approach that has a zero false discovery rate (no false positives) with high probability for any level of noise while it detects the exact location of sparse signals when the noise is not too large.
Anesthesia-induced loss of consciousness disrupts auditory responses beyond primary cortex
Authors: Krom Aaron J. , Marmelshtein Amit , Gelbard-Sagiv Hagar , Tankus Ariel , Hayat Hanna , Hayat Daniel , Matot Idit , Strauss Ido , Fahoum Firas , Soehle Martin , Boström Jan , Mormann Florian , Fried Itzhak , Nir Yuval 
Published: May 2020

Significance
We studied how anesthesia-induced loss of consciousness (LOC) affects processing of external sensory events at single-neuron resolution in epilepsy patients implanted with depth electrodes for clinical treatment. Just before electrode removal, patients were lightly anesthetized with propofol while we presented sounds around LOC. We found that auditory responses were widespread across the brain during wakefulness. Upon LOC, robust responses persisted in primary auditory cortex while responses in high-order auditory regions were mostly attenuated. In addition, gamma power responses in local field potentials strengthened upon LOC, in contrast to spiking activities. The disruption in sensory signaling beyond primary cortices highlights impaired cortical connectivity as a key factor in LOC and may guide future development of anesthesia monitors and anesthetic agents.
Indirect reciprocity with simple records
Authors: Clark Daniel , Fudenberg Drew , Wolitzky Alexander 
Published: May 2020

SignificanceIndirect reciprocity is a foundational mechanism of human cooperation, and understanding the social structures that allow it to arise continues to be a core issue in both the social sciences and evolutionary biology. This paper analyzes a model of indirect reciprocity in steady-state equilibria, where players observe only their partners’ records, and each individual’s record depends on the individual’s own past behavior alone. We show that tolerant trigger strategies based on these simple records can robustly support positive social cooperation in games with sufficient “strategic complementarity,” both in the prisoner’s dilemma and in some multiplayer public goods games, and we show that the resulting cooperative equilibria have strong stability properties.
Pore-size dependence and slow relaxation of hydrogel friction on smooth surfaces
Authors: Cuccia Nicholas L. , Pothineni Suraj , Wu Brady , Méndez Harper Joshua , Burton Justin C. 
Published: May 2020

Significance
Hydrogel’s slippery surface is derived from its cohabited nature: a sparse, cross-linked polymer network filled with fluid. However, the link between hydrogel’s microscopic properties and its macroscopic friction is not well understood. Here we show that when hydrogel is dragged along a smooth, wet solid surface, the friction is controlled by hydrodynamic interactions within the gel’s polymer network. When dragged very slowly, fluid flows through the porous gel, dominating hydrogel’s friction. When dragged more quickly, the friction coefficient abruptly decreases and exhibits transient, slow relaxation over long timescales. Stretching of entangled polymers at the hydrogel’s sliding interface explains this behavior. These results may guide molecular design principles for hydrogel materials including soft contact lenses, artificial joints, and soft robotic devices.
Sperm proteins SOF1, TMEM95, and SPACA6 are required for sperm−oocyte fusion in mice
Authors: Noda Taichi , Lu Yonggang , Fujihara Yoshitaka , Oura Seiya , Koyano Takayuki , Kobayashi Sumire , Matzuk Martin M. , Ikawa Masahito 
Published: May 2020

SignificanceThe sperm−oocyte fusion step is important to transport the male genome into oocytes. So far, IZUMO1 and FIMP have been identified as fusion-related proteins in spermatozoa, but the molecular mechanisms underpinning sperm−oocyte fusion and all of the proteins required for this essential process remain unclear. In this study, using CRISPR-Cas9−mediated gene knockouts in mice, we discover that sperm proteins SOF1, TMEM95, and SPACA6 are required for sperm−oocyte fusion and male fertility. As these genes are conserved among mammals including human, they may explain not only the sperm−oocyte fusion process but also idiopathic male infertility and be unique targets for contraception.
Identifying sequence perturbations to an intrinsically disordered protein that determine its phase-separation behavior
Authors: Schuster Benjamin S. , Dignon Gregory L. , Tang Wai Shing , Kelley Fleurie M. , Ranganath Aishwarya Kanchi , Jahnke Craig N. , Simpkins Alison G. , Regy Roshan Mammen , Hammer Daniel A. , Good Matthew C. , Mittal Jeetain 
Published: May 2020

Significance
Membraneless organelles are assemblies of highly concentrated biomolecules that form through liquid–liquid phase separation. These assemblies are often enriched in intrinsically disordered proteins (IDPs), which play an important role in driving phase separation. Understanding the sequence-to-phase behavior relationship of these disordered proteins is important for understanding the biochemistry of membraneless organelles, as well as for designing synthetic organelles and biomaterials. In this work, we explore a model protein, the disordered N-terminal domain of LAF-1, and highlight how three key features of the sequence control the protein’s propensity to phase-separate. Combining state-of-the-art simulations with experiments, we find that phase behavior of this model IDP is dictated by the presence of a short conserved domain, charge patterning, and arginine–tyrosine interactions.
Biased signaling by endogenous opioid peptides
Authors: Gomes Ivone , Sierra Salvador , Lueptow Lindsay , Gupta Achla , Gouty Shawn , Margolis Elyssa B. , Cox Brian M. , Devi Lakshmi A. 
Published: May 2020

Significance
There are >20 different endogenous opioid peptides derived from the three precursors proopiomelanocortin, proenkephalin, and prodynorphin; a long-standing question is the biological utility of having this variety of peptides. We addressed this question by systematically evaluating ligand binding and signaling properties of the peptides at each of the three opioid receptor types. Contrary to the prevailing notion, we show that all of the peptides bind and activate the three opioid receptors and that shorter β-endorphin peptides exhibit agonistic activity. Finally, we demonstrate that some endogenous peptides favor particular signaling pathways at the three receptors leading to biased signaling. These findings highlight the complexity of signaling where multiple opioid receptors are expressed and/or many opioid peptides are released.
Preventing presbycusis in mice with enhanced medial olivocochlear feedback
Authors: Boero Luis E. , Castagna Valeria C. , Terreros Gonzalo , Moglie Marcelo J. , Silva Sebastián , Maass Juan C. , Fuchs Paul A. , Delano Paul H. , Elgoyhen Ana Belén , Gómez-Casati María Eugenia 
Published: May 2020

Significance
Age-related hearing loss is the most common cause of hearing loss. Here, we show that medial olivocochlear feedback mediates resistance to age-related hearing loss—presbycusis—and that this occurs via the α9α10 cholinergic nicotinic receptor complexes on outer hair cells. These findings are particularly promising because they provide a proof of principle supporting the enhancement of the medial olivocochlear system as a viable approach for prevention or treatment of age-related hearing loss. Therefore, it opens the way to the design of novel positive modulators of α9α10 nAChR receptors for clinical use in the prevention of both noise and age-related hearing loss.