bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prevalence of Antibiotic-Resistant Bacteria in the Environment of Poultry Farms
Authors: Veloo Yuvaneswary , Thahir Syahidiah Syed Abu , Shaharudin Rafiza , Rajendiran Sakshaleni , Hock Lim Kuang , Muthu Vickneshwaran , Ahmad Norazah 
Published: July 2020

Abstract
Background

The occurrence and spread of antibiotic-resistant bacteria (ARB) due to the high demand in poultry industries are of great public health concern. Indiscriminate and abusive use of various antibiotics on a large scale causes antibiotic resistance (AMR) in animal-associated bacteria that may be pathogenic to humans. These bacteria are widely disseminated in the environment via animal waste. This study was therefore designed to assess the prevalence of multiple antibiotic resistance (MAR) among the environmental bacteria in poultry farms and to determine the risk contamination category of poultry by calculating the multiple antibiotic resistance index (MARI).
Results

More than half (58.2%) of the 511 total bacteria had MAR, and a number of bacteria were resistant to cefazolin (86.8%), fusidic acid (84.6%), ampicillin (79.3%), clindamycin (65.5%) and erythromycin (63.7%). These antibiotics are listed under the WHO’s criteria of critically and highly important antibiotics in human medicine. In this study, 39.53% of the MARI values, which indicate the contamination level in the environment, indicated a high risk, while 14.48% were ambiguous.
Conclusion

These results therefore have shown that MAR is present not only among humans and animals but also in environmental bacteria. The high prevalence of MAR and the MARI values, together with the resistance patterns of each bacterium, indicate various effects, including possible occupational risks among workers. This study provides an introduction to the AMR of bacteria in the environment. Further studies are needed to observe the horizontal transfer of the resistance gene and the overall mobile genetic elements in environmental bacteria.
Skin Microbiota Dynamics Following B. subtilis formulation Challenge
Authors: Moskovicz Veronica , Ben-El Rina , Horev Guy , Mizrahi Boaz 
Published: July 2020

Abstract
Background
Modulating the microbiota is a leading-edge strategy for the restoration and maintenance of a healthy, balanced environment. The use of health-promoting bacteria is emerging as an alternative for skin microbiota intervention, showing promising results. Bacillus subtilis, with its non-pathogenic and antibacterial properties, has great potential as a microbiota-manipulating agent. Here, we investigate the experimental manipulation of the skin microbiota using B. subtilis incorporated into a supportive Pluronic F-127 hydrogel formulation. Indeed, the formulation is of prime importance for dermal bacterial delivery, determining the ability of live bacteria to become established at the desired action site.
Results
The B. subtilis challenge induced a shift in the composition and abundance of the skin microbiota. Containment of B. subtilis in the Pluronic F-127 hydrogel accelerated bacterial modulation compared with free B. subtilis. The abundance of both Staphylococcus and Corynebacterium spp. was altered as a result of the live bacterial intervention: the abundance of Corynebacterium increased while that of Staphylococcus decreased. Four days after last application of the B. subtilis formulation, B. subtilis counts returned to its initial level.
Conclusions
B. subtilis intervention can induce a shift in the skin microbiota, with an increased presence of commensal, benefit-associated bacteria and a reduced abundance of pathogen-containing bacterial genera. Containment of B. subtilis in Pluronic hydrogel accelerates the microbial alteration, probably by facilitating bacterial attachment and supporting continuous growth. Our results reveal the ability of B. subtilis in Pluronic to modulate the skin microbiota composition, suggesting that the formulation holds therapeutic potential for skin disease treatment.
Clinical Outcomes of Chemotherapy in Patients with Undifferentiated Carcinoma of the Pancreas: A Retrospective Multicenter Cohort Study
Authors: Imaoka Hiroshi , Ikeda Masafumi , Maehara Kosuke , Umemoto Kumiko , Ozaka Masato , Kobayashi Satoshi , Terashima Takeshi , Inoue Hiroto , Sakaguchi Chihiro , Tsuji Kunihiro , Shioji Kazuhiko , Okamura Keiya , Kawamoto Yasuyuki , Suzuki Rei , Shirakawa Hirofumi , Nagano Hiroaki , Ueno Makoto , Morizane Chigusa , Furuse Junji 
Published: July 2020

Abstract
Background: Undifferentiated carcinoma (UC) of the pancreas is a rare subtype of pancreatic cancer. Although UC has been considered a highly aggressive malignancy, no clinical studies have addressed the efficacy of chemotherapy for unresectable UC. Therefore, we performed multicenter retrospective study to investigate the efficacy of chemotherapy in patients with UC of the pancreas.Methods: This multicenter retrospective cohort study was conducted at 17 institutions in Japan between January 2007 and December 2017. A total of 50 patients treated with chemotherapy were analyzed.Results: The median overall survival (OS) in UC patients treated with chemotherapy was 4.08 months. The details of chemotherapy in first-line treatment were as follows: gemcitabine (n=24), S-1 (n=12), gemcitabine plus nab-paclitaxel (n=6), and other treatment (n=8). The median progression-free survival (PFS) was 1.61 months in the gemcitabine group, 2.96 months in the S-1 group, and 4.60 months in the gemcitabine plus nab-paclitaxel group. Gemcitabine plus nab-paclitaxel significantly improved PFS compared with gemcitabine (p=0.014). The objective response rate (ORR) was 4.2% in the gemcitabine group, 0.0% in the S-1 group, and 33.3% in the gemcitabine plus nab-paclitaxel group. Gemcitabine plus nab-paclitaxel also showed a significantly higher ORR compared with both gemcitabine and S-1 (gemcitabine plus nab-paclitaxel vs. gemcitabine: p=0.033; gemcitabine plus nab-paclitaxel vs. S-1: p=0.034). A paclitaxel-containing first-line regimen significantly improved OS compared with a non-paclitaxel-containing regimen (6.94 months vs. 3.75 months, respectively; p=0.041). After adjustment, use of a paclitaxel-containing regimen in any line was still an independent predictor of OS (hazard ratio for OS, 0.221; 95% confidence interval, 0.076 – 0.647; p=0.006) in multiple imputation by chained equation.Conclusions: The results of the present study indicate that paclitaxel-containing regimen would offer relatively longer survival, and it is considered a reasonable option for treating patients with unresectable UC.
Survival After Combined Hepatic Metastasectomy and Chemotherapy in Patients with Concurrent Hepatic and Extrahepatic Colorectal Metastases: A SEER Database Analysis.
Authors: Uthman Inas , Fouad Ahmed M , Khaled Islam , Ahmed Ihab S. , Faisal Mohammed 
Published: July 2020

Abstract
Purpose: Colorectal cancer is the second leading cause of death among all cancers worldwide. Hepatic metastases exist in about 50% of colorectal cancer patients. The purpose of this study was to assess the effect of combined hepatic metastasectomy and chemotherapy on the overall survival in patients with concurrent hepatic and extra-hepatic disease.Methods: 2533 patients from the US Surveillance, Epidemiology, and End Results (SEER) database with concurrent colorectal liver metastasis (CRLM) and extra-hepatic disease (EHD) between January 1, 2010, and December 31, 2014, were retrieved. Survival analysis with Kaplan-Meier and Cox-regression was performed to assess the effect of combined hepatic metastasectomy and chemotherapy on 5-year survival. Results: Two-hundred and fourteen (8.4%) patients underwent combined hepatic metastasectomy and chemotherapy. The median survival time among patients underwent combined hepatic metastasectomy, and chemotherapy was significantly higher than chemotherapy alone (24 vs. 21 months; p < 0.0001). Furthermore, older age at diagnosis (≥ 60 years), American Indian/Alaska Native race, primary sites at the recto-sigmoid colon, sigmoid colon, and descending colon, grade III, and the presence of bone metastases were all significantly associated with higher 5-year mortality. Patients who underwent combined hepatic metastasectomy and chemotherapy were significantly associated with 22.2% less 5-year mortality compared to patients who received chemotherapy alone. Conclusion: Combined hepatic metastasectomy with chemotherapy in CRLM patients with EHD yields better survival than chemotherapy only.
Resection Of A Retroperitoneal Cancer Of Unknown Primary Site After Chemoradiation Therapy: A Case Report
Authors: Suetsugu Tomonari , Matsuhashi Nobuhisa , Tsuchiya Hiroshi , Takahashi Takao , Fukada Masahiro , Tanaka Hideharu , Iwata Yoshinori , Yasufuku Itaru , Sato Yuta , Imai Takeharu , Imai Hisashi , Tanaka Yoshihiro , Okumura Naoki , Yamaguchi Kazuya , Yoshida Kazuhiro 
Published: July 2020

Abstract
Background: Complete recovery from retroperitoneal squamous cell carcinoma of unknown primary site treated by multidisciplinary therapy is extremely rare.Case presentation: A 78-year-old man was referred to our hospital due to a mass measuring 8 cm in size in the left pelvic retroperitoneal area, which was diagnosed as a cancer of unknown primary site. The pathological type was squamous cell carcinoma. The left iliac artery/vein and ureter were involved in the tumor, and the tumor caused severe left leg pain. Although inflammation and symptoms were severe, palliative radiotherapy was considered. After that, biweekly combined therapy with docetaxel, cisplatin, and fluorouracil was planned. After 5 courses of chemotherapy, the tumor diameter reduced from 11.6 cm to 4 cm in size. In addition, the border between the iliac vessels, urinary tract and tumor became apparent. The patient underwent radical resection of the tumor 8 months after the treatment started. The left ureter and the external/internal iliac artery were preserved, but the external iliac vein was sacrificed because of the possibility of tumor invasion. The postoperative course was free of complications, and the patient was discharged 10 days after the operation. The histopathological findings showed no residual viable tumor cells or foreign body-type giant cells with necrosis. The pathological effect of chemotherapy was defined as Grade 3 (pathological complete response). The patient has experienced no recurrence or distant metastasis for 4 years.Conclusions: Multidisciplinary therapy succeeded in treating a retroperitoneal squamous cell carcinoma of unknown primary site with preservation of organ function.
Anastomosis Technique For Pancreatojejunostomy And Early Removal Of Drainage Tubes May Reduce Postoperative Pancreatic Fistula
Authors: Kawaida Hiromichi , Kono Hiroshi , Amemiya Hidetake , Hosomura Naohiro , Watanabe Mitsuaki , Saito Ryo , Nakata Yuuki , Shoda Katsutoshi , Shimizu Hiroki , Furuya Shinji , Akaike Hidenori , Kawaguchi Yoshihiko , Sudo Makoto , Matsuda Masanori , Itakura Jun , Fujii Hideki , Ichikawa Daisuke 
Published: July 2020

Abstract
Background: Postoperative pancreatic fistula (POPF) is one of the most serious complications after pancreaticoduodenectomy (PD). Various factors have been reported as POPF risks, but the most serious of these is soft pancreas. To reduce POPF occurrences, many changes to the PD process have been proposed. This study evaluates short-term results of anastomosis technique for PD.Methods: In total, 120 patients with soft pancreases who had undergone PD at Yamanashi University between January 2012 and December 2019 were retrospectively analyzed. We divided these patients into two groups depending on the time PD was performed: a conventional group (n=67) and a modified group (n = 53).Results: The rate of clinically relevant POPF was significantly lower in the modified group than that in the conventional group (1.9% vs 22.4%, p < 0.001), with there being only one case of POPF in the modified group. There were no cases of POPF-related hemorrhaging in the modified group. On the third day after the operation, the amylase levels in the drainage fluid for the modified group had reduced to one third of those in the conventional group (1696 vs 643 U/L). Multivariate analysis showed that the modified method was the independent predictors to prevent clinical POPF (p = 0.004).Conclusions: Our novel anastomosis technique for pancreatojejunostomy reduced POPF in PD, especially in cases where the patient had a soft pancreas.
How to Choose the Best Procedure Type for Duodenal Neuroendocrine Tumors With a Maximum Diameter of 1 to 2 Cm a Propensity Score Matching Analysis Based on the SEER Database
Authors: Tang Huanyu , xie jiebin , Pang Yueshan , Hua Xia , Li xun 
Published: July 2020

Abstract
Background: The treatment plan for duodenal neuroendocrine tumors (d-NETs) with a diameter between 1 and 2 cm is still controversy. Aim: To compare the effects of local endoscopic resection and radical resection on the prognosis of d-NETs with a maximum diameter of 1-2 cm. Methods: 286 eligible patients were identified from the SEER database. Propensity score matching (PSM) was done to match patients 1:1 on clinicopathological characteristics. Kaplan-Meier analysis was used to analyze the factors affecting the prognosis.Results: Before PSM, there was no significant difference in the cancer-specific survival (CSS) between the two groups (P = 0.595), but the tumor size, T stage, N stage, and M stage were significantly different between the two groups (all P < 0.05). After 1:1 PSM, the differences in clinicopathological characteristics between the two groups were significantly reduced (all P > 0.05). Survival analysis showed that only the tumor grade was correlated with the prognosis (P = 0); surgical method and other clinicopathological characteristics were not correlated with the prognosis (all P > 0.05). Conclusion: The surgical approach had no significant effect on the prognosis of d-NET patients with a maximum diameter of 1-2 cm and without lymph node metastasis.
Prognosis and Risk Factors for Malignant Peripheral Nerve Sheath Tumor: A Systematic Review and Meta-analysis
Authors: Cai Zhenyu , Tang Xiaodong , Liang Haijie , Yang Rongli , Yan Taiqiang , guo wei 
Published: July 2020

Abstract
Background: No available meta-analysis was printed to systematically introduce the MPNST clinic outcome and risk factors based on largely pooled data. This systematic review and meta-analysis aimed to investigate 5-year OS rate, 5-year EFS rate and LR rate for MPNST, and to assess potential risk factors for prognosis.Methods: Electronic articles published between January 1, 1966 and February 29, 2020, were searched and critically evaluated. The authors independently reviewed the abstracts and extracted data for 5-year OS rate, 5-year EFS rate, LR rate, and potential risk factors for prognosis.Results: Twenty-eight literatures were finally included for meta-analysis. The pooled 5-year OS rate, 5-year EFS rate, and LR rate were 49%, 37%, and 37%, respectively. The significant prognostic factors for survival were NF-1 status, tumor size, depth, location, malignant grade, margin status, chemotherapy, and radiotherapy. Age and sex were not associated with survival.Conclusion: Survival and local recurrence of MPNST are poor. Worse prognosis is mainly associated with NF 1 mutation, large size, deep to fascia, high grade, metastases and location (trunk and head & neck). Complete resection with adequate surgical margins is the mainstay protective factor of MPNST patients, following necessary adjuvant therapies.
Knockdown of Long Non-coding RNA UCA1 Represses Gallbladder Cancer Advancement by Regulating SPOCK1 Expression via Sponging miR-613
Authors: Zhang Tao , Chen Lijian , Xu Xundi , Shen Chao 
Published: July 2020

Abstract
OBJECTIVE : Patients with advanced gallbladder cancer (GBC) have a lower 5-year survival rate. Long non-coding RNA urothelial carcinoma associated 1 (UCA1) and miR-613 are involved in the progression of various cancers. This study was to explore the regulatory mechanism between UCA1 and miR-613 in GBC. METHODS: The expression levels of UCA1, miR-613, and SPOCK1 mRNA were detected using qRT-PCR. Cell proliferation, migration, invasion, and apoptosis were determined with MTT, transwell, or flow cytometry assays. The levels of SPOCK1 protein, Bax, cleaved-casp-3, and Bcl-2 were determined by western blot analysis. The relationship between miR-613 and UCA1 or SPOCK1 was verified via dual-luciferase reporter and/or RNA immunoprecipitation (RIP) assays. The role of UCA1 in vivo was confirmed by xenograft assay. RESULTS: UCA1 and SPOCK1 were upregulated while miR-613 was downregulated in GBC tissues and cells. UCA1 silencing blocked tumor growth in vivo, impeded cell proliferation, migration, invasion, and induced cell apoptosis in GBC cells in vitro. Notably, UCA1 acted as a sponge for miR-613, which targeted SPOCK1 in GBC cells. Moreover, miR-613 repressed cell proliferation, migration, invasion, and accelerated cell apoptosis in GBC cells. UCA1 enhancement reversed miR-613 mimic-mediated influence on proliferation, migration, invasion, and apoptosis of GBC cells. UCA1 regulated SPOCK1 expression through miR-613. Furthermore, SPOCK1 elevation overturned UCA1 silencing-mediated the malignant behaviors of GBC cells. CONCLUSION: UCA1 knockdown suppressed GBC progression via downregulating SPOCK1 via sponging miR-613, providing an evidence for UCA1 as a target for GBC treatment.
Cultural Adaptation of the PTSD Checklist- Military version (PCL-M) among Army Personnel in Ghana
Authors: Agah Nathanael Nartey 
Published: July 2020

Abstract
Background: The type of deployment duties performed by military personnel differs across the world, as some perform combat related deployment, others are sent on peacekeeping duties. Due to the difference in events experienced when performing any of the deployment duties as combat veterans are more likely to encounter traumatic events than peacekeeping duties personnel, the occurrence of PTSD symptoms might differ. The study aims to construct a PTSD Checklist – Military version that is relevant to peacekeeping duty army personnel in Ghana (PCL-M-G), on the basis of the original scale by Weathers and colleagues.Methods: A cross-sectional study was undertaken with a purposive and convenient sample of 189 army personnel of the Ghana Armed Forces who have returned from peacekeeping duties within the last year as at the time of data collection. The original scale containing 17 items was administered for data collection. Principal Component Analysis (PCA) with Varimax rotation was used in the extraction of factor structures. Cronbach’s Alpha was used to determine the internal consistency of the scale. Bivariate correlation was used to examine the external validity of the scale. Results: 12 items with three factors, namely, Hyperarousal (Cronbach alpha= .92), Re-experience (Cronbach alpha= .92) and Avoidance (Cronbach alpha= .79) was identified. The three factors altogether explained 77.21% of the total variance. The scale had a significant positive correlation with post deployment transition experience over at most a year (r = .33), as this shows evidence of construct and discriminatory validity. Even though the factors identified in this study are similar to the original scale, there are some differences, indicating the significations of cultural diversity.Conclusion: The current study of PCL=M among Ghanaian army personnel identified difference with regards to item factor loadings and dimension. It is suggested that the scale would be validated locally before it is used. The PCL-M-G is reliable and valid, therefore, researchers, clinicians as well as other health workers can cautiously use this scale when assessing PTSD symptoms among Ghanaian military personnel.
Total Body Water (TBW) Percentage and 3rd Space Water Were Risk Factors for Recruit Injuries in Lower Limbs: A Case-Control Study
Authors: Ouyang Junjie , Yang Jing , Jiang Wanling , Li Bing , Jin Kexing , Chen Liang , Luo Xiaoqing , Chang Jinhong , Tan Qiaoyan , Kuang Liang , Xie Yangli , Liu Mi , He Mei , Chen Hangang , Meng Tao , Hu Yan , Chen Lin , Ni Zhenhong , Du Xiaolan 
Published: July 2020

Abstract
Background: Recruit training injuries have caused serious problems for troop training and medical support. The lower limbs is the site where recruit injuries occur the most. Bio-impedance (BIA) measures body composition quickly and accurately. Our aim was to identify the risk factors for lower limbs training injuries to recruits due to body composition.Methods: A total of 282 recruits were included. Before training, use BodyStat QuadScan 4000 multifrequency BIA system to measure the body composition of recruits. After training, they were divided into two groups according to the occurrence of lower limb training injuries. The basic characteristics of the two recruits were compared by Wilcoxon rank sum test. Receiver operator characteristic (ROC) curves was performed on the indicators with statistical difference between the two groups to find the cutoff point. Finally, multivariate logistic regression analysis was used to find the risk factors of lower limb training injuries.Results: Compared with the lower limb uninjured group, the lean mass percentage (P = 0.003), TBW percentage (P = 0.010), extracellular water (ECW) percentage (P = 0.023), intracellular water(ICW) percentage (P = 0.027), 3rd space water (P = 0.021) and basal metabolic rate(BMR)/total weight (P = 0.014) of the lower limb injury group was higher. On the contrary, the body fat percentage (P = 0.003) and body fat mass index (BFMI) (P = 0.005) of the lower limb injury group was lower. The results of multivariate logistic regression analysis showed that TBW percentage > 65.350% (P = 0.050, OR=2.085) and 3rd space water >0.950 (P = 0.045, OR=2.342) were independent risk factors for lower limb injuries.Conclusions: TBW percentage> 65.35% and 3rd space water >0.950 were independent risk of lower limb training injuries. These recruits need to be paid more attention during training.
Dactylitis in psoriatic arthritis – prevalence and reliability of ultrasound lesions in a clinical setting
Authors: Felbo Sara Helena Kamp , Østergaard Mikkel , Sørensen Inge J. , Terslev Lene 
Published: July 2020

Abstract
Objectives. To investigate the prevalence of ultrasound elementary lesions in dactylitis in psoriatic arthritis (PsA), and the reliability of scoring these lesions in a clinical setting.Methods. In 31 patients with PsA and clinical dactylitis, ultrasound assessment of the affected digit was performed using grey-scale and color Doppler mode. One examiner scanned all patients and a 2nd examiner scanned 10 patients for inter-reader reliability. The following lesions were evaluated: subcutaneous edema; soft tissue thickening; synovitis of the digital joints; tenosynovitis of the flexor tendon; enthesitis at the deep flexor tendon and the extensor tendon entheses; and paratenonitis of the extensor tendon. A dactylitis sum-score was calculated. Findings in clinically tender and non-tender digits were compared.Results. The most frequent lesions were soft tissue thickening (81%) and subcutaneous edema (74%) followed by synovitis (56–68%) and flexor tenosynovitis (52%). Color Doppler was most frequently found subcutaneously (55%) and around the flexor tendons (45%). All lesions were typically found in combinations, most commonly subcutaneous edema and synovitis (71%), subcutaneous edema and flexor tenosynovitis (52%), and all three in combination (52%). Tender digits had a higher dactylitis sum-score and numerically higher prevalence of most lesions than non-tender digits. Intra- and inter-reader agreements were moderate to excellent, though lower for few components of digital enthesitis, especially hypoechogenicity.Conclusion. Dactylitis in PsA appears to encompass several pathologies, most often subcutaneous changes combined with synovitis and/or flexor tenosynovitis. Reliability of scoring established ultrasound lesions of dactylitis in a clinical setting is moderate-excellent.