bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparison of species richness and detection between line transects, ground camera traps, and arboreal camera traps
Authors: Moore J. F. , Pine W. E. , Mulindahabi F. , Niyigaba P. , Gatorano G. , Masozera M. K. , Beaudrot L. 
Published: March 2020

AbstractMonitoring trends in the occurrence of species over time is important for informing conservation plans and concurrent management actions. Understanding the effectiveness of field methodologies for collecting accurate and precise data is crucial for optimizing allocation of sampling effort and resources. In this study, we compared mammalian species richness and detection probabilities between three field methodologies: line transects, ground camera traps and arboreal camera traps in Nyungwe National Park, Rwanda. Arboreal camera traps may be suitable for monitoring mammal communities with arboreal species, but their relative effectiveness compared to the more common field methods, line transects and ground camera traps, is relatively unknown. Using single‐season occupancy models with multi‐species data and single‐species multi‐method occupancy models, we estimated mammalian species richness and detection probability for each method and combination of methods. In addition, we estimated single‐species occupancy and detection probability by method for six diurnal primate species. And, we tested for the effect of height on a tree on estimated occupancy probability and detection probability for arboreal camera traps. Overall, for all species the combination of ground and arboreal cameras was the most effective methodology in terms of highest estimates of occupancy and detection coupled with highest precision. However, for the six primate species the most effective method differed between species. The height of the arboreal camera trap in the tree did not significantly affect estimates of occupancy or detection. We suggest using all three field methods concurrently to maximize detection of all species; however, if only two methods can be deployed combining arboreal and ground cameras provided the highest and most precise estimates of occupancy and detection. The addition of arboreal camera traps could improve detection of species and improve future species monitoring programs.
Adaptive amelogenesis during unimpeded eruption of rat mandibular incisor
Authors: Risnes Steinar , Goldberg Michel 
Published: March 2020

AbstractThe aim of the study was to see whether the length of the enamel secretion zone in unimpeded rat incisors, measured precisely, is in agreement with the observed decrease in enamel thickness. Unimpeded eruption of mandibular incisors of five experimental and two control rats was induced by cutting off the erupted part of the incisors three times per week for 5 weeks. The length of the zone of enamel secretion in unimpeded and impeded control incisors was measured on longitudinal and serial transverse histological sections of fixed, demineralised and embedded hemimandibles. Impeded contralateral incisors were also included in the study. The length of the zone of enamel secretion in unimpeded incisors showed an increase to 8,398 ± 558 µm, that is 161% of the length in control incisors (5,213 ± 95 µm). The contralateral incisor showed a reduction in eruption rate, in length of the secretion zone, and the whole tooth was shifted somewhat apically. The measured length of the secretion zone is in agreement with the observed thickness of enamel (98 µm) in unimpeded incisors. The reduced eruption rate and the apical shift of the contralateral incisor are probably due to an increased occlusal load.
Effects of supplementation with β‐carotene on the growth performance and intestinal mucosal barriers in layer‐type cockerels
Authors: Hui Junnan , Li Lingxi , Li Ruonan , Wu Min , Yang Yu , Wang Jinghui , Fan Yuanyuan , Zheng Xin 
Published: March 2020

Abstractβ‐carotene is a robust modulator of mucosal barriers, and it can amplify the immunoglobulin A (IgA) response via the retinoic acid (RA)–mediated pathway. We investigated the influence of β‐carotene on intestinal barriers in layer‐type cockerels. In this study, β‐carotene has a positive influence on growth performance and intestinal morphology. β‐carotene remarkably enhanced serum secretory immunoglobulin A (sIgA) levels, jejunal mucosal sIgA, and IgA concentrations. β‐Carotene significantly enhanced mRNA expression levels of IgA, CC chemokine receptor‐9 (CCR9), polymeric immunoglobulin receptor (pIgR), and retinoic acid receptor α (RARα) in the ileal tissues and pIgR in the jejunal tissues. β‐Carotene improves mRNA expression of intestinal barrier‐related proteins including: mucin‐2 (MUC‐2), zonula occludens‐2 (ZO‐2), occludins (OCLN), and zonula occludens‐1 (ZO‐1) in the ileal tissues. Moreover, β‐carotene decreased the levels of Escherichia coli and elevates the levels of Lactobacillus. The results indicate that β‐carotene can promote growth performance and contribute to the gradual development of intestinal barriers in Hyline Brown chicks. This study enriches our knowledge about the effects of β‐carotene on intestinal barrier and highlights a theoretical basis of β‐carotene application in the poultry industry.
Early experience using salvage radiotherapy for relapsed/refractory non‐Hodgkin lymphomas after CD19 chimeric antigen receptor (CAR) T cell therapy
Authors: Imber Brandon S. , Sadelain Michel , DeSelm Carl , Batlevi Connie , Brentjens Renier J. , Dahi Parastoo B. , Giralt Sergio , Park Jae H. , Sauter Craig , Scordo Michael , Shah Gunjan , Perales Miguel‐Angel , Palomba M. Lia , Yahalom Joachim 
Published: March 2020

SummaryRadiotherapy is potentially an important salvage strategy post‐chimeric antigen receptor T cell therapy (CART), but limited data exist. We reviewed 14 patients treated with salvage radiation post‐CART progression (SRT). Most received SRT for first post‐CART relapse (71%) to sites previously PET‐avid pre‐CART (79%). Median overall survival (OS) post‐SRT was 10 months. Post‐SRT, six localized relapses achieved 100% response (3 = complete, 3 = partial), with improved freedom from subsequent relapse (P = 0·001) and OS (P = 0·004) compared to advanced stage relapses. Three were bridged to allogeneic transplantation; at analysis, all were alive/NED. SRT has diverse utility and can integrate with novel agents or transplantation to attempt durable remissions.
Disease pharmacokinetic–pharmacodynamic modelling in acute intermittent porphyria to support the development of mRNA‐based therapies
Authors: Parra‐Guillen Zinnia P. , Fontanellas Antonio , Jiang Lei , Jericó Daniel , Martini Paolo , Vera‐Yunca Diego , Hard Marjie , Guey Lin T. , Troconiz Iñaki F. 
Published: March 2020

Background and PurposeAcute intermittent porphyria (AIP) results from haplo‐insufficiency of the porphobilinogen deaminase (PBGD) gene encoding the third enzyme in the haem biosynthesis pathway. As liver is the main organ of pathology for AIP, emerging therapies that restore enzyme hepatic levels are appealing. The objective of this work was to develop a mechanistic‐based computational framework to describe the effects of novel PBGD mRNA therapy on the accumulation of neurotoxic haem precursors in small and large animal models.Experimental ApproachLiver PBGD activity data and/or 24‐hr urinary haem precursors were obtained from genetic AIP mice and wild‐type mice, rats, rabbits, and macaques. To mimic acute attacks, porphyrogenic drugs were administered over one or multiple challenges, and animals were used as controls or treated with different PBGD mRNA products. Available experimental data were sequentially used to build and validate a semi‐mechanistic mathematical model using non‐linear mixed‐effects approach.Key ResultsThe developed framework accounts for the different biological processes involved (i.e., mRNA sequence, release from lipid nanoparticle and degradation, mRNA translation, increased PBGD activity in liver, and haem precursor metabolism) in a simplified mechanistic fashion. The model, validated using external data, shows robustness in the extrapolation of PBGD activity data in rat, rabbit, and non‐human primate species.Conclusion and ImplicationsThis quantitative framework provides a valuable tool to compare PBGD mRNA drug products during early preclinical stages, optimize the amount of experimental data required, and project results to humans, thus supporting drug development and clinical dose and dosing regimen selection.
Contrasting population genetic structure of two sympatric species of Anthurium (Araceae) along elevation in an Andean mountain forest
Authors: Cuartas‐Hernández Sandra E. , Moreno‐Betancur Deisy J. 
Published: March 2020

AbstractThe topography of the tropical Andean forest is steep, resulting in physical conditions that change with the elevation, thus driving a high turnover of insect pollinator species over short distances, which may lead to localized gene dispersal, promoting genetic divergence between plant populations distributed along the elevation gradient. In this study, we characterized the population genetic structure of Anthurium caucanum and A. panduriforme, two closely related species that coexist along the elevation gradient in the Andean forest but differ in inflorescence size and abundance of flower visitors. The sampling of both species covered seven elevation bands, from 2,200 to 2,900 MASL. Five microsatellite loci were used to genotype the sampled individuals. Our results indicated that heterozygosity was high for both species. Genotypic richness was significantly lower for A. caucanum (G/N = 0.45) compared with A. panduriforme (G/N = 0.88), while population differentiation was strong for A. caucanum (Fst = 0.23) but low for A. panduriforme (Fst = 0.03). Assignment analysis suggested a low rate of immigration events for A. caucanum populations and a high gene flow between A. panduriforme populations. Based on the seed production of bagged inflorescences, A. caucanum demonstrated the ability to self‐pollinate, while A. panduriforme was considered self‐incompatible. In addition, genotypic richness decreased with elevation for A. caucanum. In summary, the contrasting population genetic structure of the two Anthurium species along the elevation gradient appear to be the result of different gene flow rates, mating systems, and life‐history traits, such as clonal growth, flowering phenology, and density.Abstract in Spanish is available with online material.
A Survey on Visualizations for Musical Data
Authors: Khulusi R. , Kusnick J. , Meinecke C. , Gillmann C. , Focht J. , Jänicke S. 
Published: March 2020

AbstractDigital methods are increasingly applied to store, structure and analyse vast amounts of musical data. In this context, visualization plays a crucial role, as it assists musicologists and non‐expert users in data analysis and in gaining new knowledge. This survey focuses on this unique link between musicology and visualization. We classify 129 related works according to the visualized data types, and we analyse which visualization techniques were applied for certain research inquiries and to fulfill specific tasks. Next to scientific references, we take commercial music software and public websites into account, that contribute novel concepts of visualizing musicological data. We encounter different aspects of uncertainty as major problems when dealing with musicological data and show how occurring inconsistencies are processed and visually communicated. Drawing from our overview in the field, we identify open challenges for research on the interface of musicology and visualization to be tackled in the future.
Histopathological features of photodamage and mast cell infiltrate in actinic cheilitis with different grades of epithelial dysplasia
Authors: Santana Thalita , Nagata Gabriela , Saturno Juvani Lago , Trierveiler Marília 
Published: March 2020

AbstractBackgroundActinic cheilitis is induced by chronic exposure to ultraviolet radiation and shows solar elastosis, a feature that has been associated with mast cell infiltrates. This study aimed to investigate the area of solar elastosis, collagen loss, and mast cell infiltrates in a series of actinic cheilitis.MethodsWe evaluated the epithelial dysplasia in 52 cases of actinic cheilitis and the solar elastosis with Weigert's resorcin‐fuchsin. Collagen loss was evaluated with Picrosirius red, analyzed under polarized microscopy, and scored from 1 to 3. Elastosis proportionate area (EPA) was calculated with image software. Mast cells were highlighted with toluidine blue stain.ResultsEPA varied from 2% to 45%, with a mean of 17.1% in the cases, with no differences among epithelial dysplasia degrees. Most cases presented collagen loss scores of 2 or 3, and higher loss of type I collagen was associated with older age. Mast cell density was higher in severe epithelial dysplasia (P = 0.002) and in high‐risk cases (P = 0.01).ConclusionActinic cheilitis presented variable EPA and marked loss of type I collagen; however, these features were not associated with the degrees of epithelial dysplasia. Besides, mast cell density increased with epithelial dysplasia worsening and this was not associated with elastosis area or collagen loss.
Disseminated tuberculosis presenting as medium‐vessel vasculitis in an immunocompromised host
Authors: Wang Jennifer Y. , Brown Ryanne A. , Pugliese Silvina , Kwong Bernice Y. , Novoa Roberto A. 
Published: March 2020

AbstractCutaneous tuberculosis is an uncommon entity with several clinical forms recognized. Histopathologically, most cases are characterized by granulomatous inflammation and caseating necrosis, although less common findings, including vasculitis, have also been described. We report a 55‐year‐old male with a history of recently diagnosed dermatomyositis receiving immunosuppression with mycophenolate mofetil and prednisone, who developed multifocal soft tissue abscesses and an indurated erythematous plaque on the back. Skin biopsy of the back revealed a necrotizing medium‐vessel vasculitis. Mycobacterium tuberculosis was detected in the skin via acid‐fast bacilli stain and confirmed by tissue culture and polymerase chain reaction. Cutaneous findings improved rapidly with antituberculosis therapy. This case illustrates an uncommon clinical and histopathologic presentation of disseminated tuberculosis.
Identification of signal peptide features for substrate specificity in human Sec62/Sec63‐dependent ER protein import
Authors: Schorr Stefan , Nguyen Duy , Haßdenteufel Sarah , Nagaraj Nagarjuna , Cavalié Adolfo , Greiner Markus , Weissgerber Petra , Loi Marisa , Paton Adrienne W. , Paton James C. , Molinari Maurizio , Förster Friedrich , Dudek Johanna , Lang Sven , Helms Volkhard , Zimmermann Richard 
Published: March 2020

In mammalian cells, one‐third of all polypeptides are integrated into the membrane or translocated into the lumen of the endoplasmic reticulum (ER) via the Sec61 channel. While the Sec61 complex facilitates ER import of most precursor polypeptides, the Sec61‐associated Sec62/Sec63 complex supports ER import in a substrate‐specific manner. So far, mainly posttranslationally imported precursors and the two cotranslationally imported precursors of ERj3 and prion protein were found to depend on the Sec62/Sec63 complex in vitro. Therefore, we determined the rules for engagement of Sec62/Sec63 in ER import in intact human cells using a recently established unbiased proteomics approach. In addition to confirming ERj3, we identified 22 novel Sec62/Sec63 substrates under these in vivo‐like conditions. As a common feature, those previously unknown substrates share signal peptides (SP) with comparatively longer but less hydrophobic hydrophobic region of SP and lower carboxy‐terminal region of SP (C‐region) polarity. Further analyses with four substrates, and ERj3 in particular, revealed the combination of a slowly gating SP and a downstream translocation‐disruptive positively charged cluster of amino acid residues as decisive for the Sec62/Sec63 requirement. In the case of ERj3, these features were found to be responsible for an additional immunoglobulin heavy‐chain binding protein (BiP) requirement and to correlate with sensitivity toward the Sec61‐channel inhibitor CAM741. Thus, the human Sec62/Sec63 complex may support Sec61‐channel opening for precursor polypeptides with slowly gating SPs by direct interaction with the cytosolic amino‐terminal peptide of Sec61α or via recruitment of BiP and its interaction with the ER‐lumenal loop 7 of Sec61α. These novel insights into the mechanism of human ER protein import contribute to our understanding of the etiology of SEC63‐linked polycystic liver disease.DatabasesThe mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository (http://www.ebi.ac.uk/pride/archive/projects/Identifiers) with the dataset identifiers: PXD008178, PXD011993, and PXD012078. Supplementary information was deposited at Mendeley Data (https://data.mendeley.com/datasets/6s5hn73jcv/2).
Experimenting with institutions in a 21st century age of “post‐truth”
Authors: Walker Dominic 
Published: March 2020

AbstractThis paper contributes to experimental geographies in the context of scientific, research‐based institutions. This focus is under‐explored but increasingly under siege in a “post‐truth”, “alternative facts” and “fake news” era in the US and UK. I respond to the current cultural, political and social challenges presented to institutions against this backdrop, arguing for a solution to these challenges: experimenting with institutions. The paper first outlines how experiments have recently become used in experimental geographies. The paper then applies these understandings of experiments to the context of institutions, arguing for engaging with new, key situations rather than necessarily with key “experts”. The paper lastly explores one example of such experiments, termed “artist‐led institutions”, and their merits for addressing the challenges outlined. I argue these artist‐led institutions demonstrate potential solutions to current institutional difficulties through expanding engagements to include more practitioners and groups rather than just “experts”, as part of a turn towards recognising more forms of expertise, and their value for wider conversations. By engaging with wider groups assembled around key situations rather than “experts”, artist‐led institutions open up the value of conversations as part of the process of knowledge‐making, rather than solely focusing on the production of outputs in pursuit of certified expertise characteristic of scientific, research‐based institutions. In doing so, the paper contributes to a discussion involving experimental geographies and institutions which may yet become increasingly audible in an age of uncertainty, as 21st Century challenges unfold.
Artificial liver treatment improves survival in patients with hepatitis B virus‐related acute‐on‐chronic liver failure: A case‐control matched analysis
Authors: Yang Lingling , Wu Tianzhou , Li Jiang , Xin Jiaojiao , Shi Dongyan , Jiang Jing , Liang Xi , Lu Yingyan , Yao Heng , Zhang Huafen , Sun Suwan , Li Tan , Mohamed Hassan Mohamed Hozeifa , Li Jiaqi , Ren Keke , Guo Beibei , Zhou Xingping , Chen Jiaxian , Hao Shaorui , Chen Jiajia , Xin Shaojie , Pan Chen , Han Tao , Chen Yongping , Lin Shumei , Duan Zhongping , Xu Xiaowei , Huang Jianrong , Chen Xin , Li Lanjuan , Li Jun ,   Chinese Group on the Study of Severe Hepatitis B (COSSH)
Published: March 2020

AimThe artificial liver support system (ALSS) is recognized as a bridge to liver transplantation in hepatitis B virus‐related acute‐on‐chronic liver failure (HBV‐ACLF) patients. However, patient survival remains unknown. We aim to assess the effects of ALSS on survival in HBV‐ACLF patients.MethodsThe clinical data of HBV‐ACLF patients receiving standard medical treatment (SMT) plus ALSS (ALSS group, n = 507) or only SMT (SMT group, n = 417) were collected for survival assessment. The main end‐points were cumulative survival rates at days 21, 28, and 90. Four different rigorous analyses were carried out to reduce bias and confounding.ResultsIn the entire cohort, the cumulative survival rates at days 21, 28, and 90 were significantly higher in patients who underwent ALSS treatment (73.3% vs. 59.6%, 69.2% vs. 56.6%, 56.5% vs. 49.1%, respectively, P < 0.01) than in those who underwent SMT only. In the 276‐pair case–control matched cohort, a significantly higher survival rate was also observed in the ALSS group than in the SMT group on days 21, 28, and 90 (72.5% vs. 60.3%, 68.3% vs. 57.4%, 55.9% vs. 48.5%, respectively, P < 0.05), especially in patients with ACLF‐1 and ‐2. By a multivariable‐adjusted analysis, ALSS treatment was associated with a significantly lower risk of mortality, especially for ACLF‐2 at days 21, 28, and 90. These findings were also confirmed through propensity score matching and inverse probability treatment weighting analysis.ConclusionsALSS treatment can improve short‐term survival and is associated with a significantly lower risk of short‐term mortality in patients with HBV‐ACLF, especially ACLF‐2.
Effects of impregnate temperature on extraction of caffeoylquinic acid derivatives from Moringa oleifera leaves and evaluation of inhibitory activity on digestive enzyme, antioxidant, anti‐proliferative and antibacterial activities of the extract
Authors: Hamed Yahya Saud , Abdin Mohamed , Chen Guijie , Akhtar Hafiz Muhammad Saleem , Zeng Xiaoxiong 
Published: March 2020

SummaryIn an ultrasonic bath and for 30 min in the presence of water as extraction solvent in a ratio of 1:10 (g/mL), effect of extraction temperature on extraction of caffeoylquinic acids (CQAs) from Moringa oleifera leaves (MOL) was investigated. Gradually increasing in extraction temperature caused a growing in mono‐CQA and declining in 3,5‐diCQA. The extract obtained at 80 °C showed the highest antioxidant capacity, and IC50 values for scavenging activities on DPPH radicals and ABTS radicals were 56.9 and 53.6 µg/mL, respectively. Compared with α‐glucosidase and α‐amylase, the extract showed the highest inhibition on pancreatic lipase (IC50 of 0.073 mg/mL). Increasing the extraction temperature improved cytotoxicity of the extract on cancer cell lines of HeLa and HepG2. Compared with gram‐negative Escherichia coli and Salmonella typhimurium, all the extracts showed higher inhibition on the growth of gram‐positive Bacillus cereus and Staphylococcus aureus. Our results suggested that use of higher extraction temperature (80 °C) improved the biological activity of MOL extract.
¿Conduce la flexibilización a menor desempleo? Un análisis empírico del mercado laboral español
Authors: HERRERO Daniel , CÁRDENAS Luis , LÓPEZ GALLEGO Julián 
Published: March 2020

ResumenEn España se llevaron a cabo dos grandes reformas laborales (2010 y 2012) bajo el supuesto de que la flexibilización del mercado laboral y la descentralización de la negociación colectiva conducirían mecánicamente a reducir el desempleo (hipótesis flexibilizadora). Este artículo destaca la centralidad de la demanda y de la composición sectorial de la economía para explicar el comportamiento de esta variable (hipótesis estructural). Mediante un análisis con datos de panel a escala subnacional, evaluamos la capacidad de ambas hipótesis para explicar la evolución del desempleo. Nuestros resultados cuestionan los supuestos de la hipótesis flexibilizadora y subrayan la importancia de los factores cíclicos y estructurales.