bluuebunny/crossref_metadata_2025_split huggingface dataset:
Following the Right: Left and Right Parties’ Influence over Multiculturalism
Authors: Westlake Daniel 
Published: February 2020

AbstractDebates over multiculturalism are increasingly partisan. The rise of the far right is forcing centre-right parties into difficult decisions over how to hold on to nationalist voters while appealing to moderates. Left parties face similar dilemmas when balancing the pressures of pro-multicultural voters against those opposed to immigration. What do these debates mean for the future of multiculturalism? Using a new, annualized version of the Multiculturalism Policy Index, this article argues that partisan consensus is important to the development of multiculturalism. It demonstrates that support from centre-right parties plays a key role in the adoption of multiculturalism policies.
Application of a fast and cost-effective ‘three-in-one’ MMR ELISA as a tool for surveying anti-MMR humoral immunity: the Hungarian experience
Authors: Böröcz K. , Csizmadia Z. , Markovics Á. , Farkas N. , Najbauer J. , Berki T. , Németh P. 
Published: February 2020

Abstract
In Hungary, between February 2017 and July 2019, 70 confirmed measles cases were reported, raising questions about the adequacy of population-level immunity. Although the assumed vaccination coverage is ≥99%, in a recent study, we detected potential gaps in the anti-measles humoral immunity. In Hungary, according to a decree by the Ministry of Public Welfare, beginning from 2021, the healthcare provider should conduct a serosurvey of anti-measles protection levels of healthcare professionals. To facilitate the compliance with this requirement, we developed a quick ‘three-in-one’ or ‘triple’ MMR (measles, mumps and rubella) indirect ELISA (IgG); an assay format that is currently not available commercially. High throughput applicability of the ‘three-in-one’ ELISA was verified using 1736 sera from routine laboratory residual samples, using an automated platform (Siemens BEP 2000 Advance). Assay verification was performed by comparing the full antigen repertoire-based ‘target’ assay with in-house ‘control’ assays using recombinant viral antigen coatings, and by validated commercially available kits. Indirect immunofluorescence was used as an independent reference method. Data were analysed using OriginLab, IBM SPSS, RStudio and MedCalc. In case of measles, we combined our current results with previously published data (Ntotal measles = 3523). Evaluation of anti-mumps and anti-rubella humoral antibody levels was based on the measurement of 1736 samples. The lowest anti-measles seropositivity (79.3%) was detected in sera of individuals vaccinated between 1978 and 1987. Considering the antigen-specific seropositivity ratios of all samples measured, anti-measles, -mumps and -rubella IgG antibody titres were adequate in 89.84%, 91.82% and 92.28%, respectively. Based on the virus-specific herd immunity threshold (HIT) values (HITMeasles = 92–95%, HITMumps = 75–86%, HITRubella = 83–86), it can be stated that regarding anti-measles immunity, certain age clusters of the population may have inadequate levels of humoral immunity. Despite the potential gaps in herd immunity, the use of MMR vaccine remains an effective and low-cost approach for the prevention of measles, mumps and rubella infections.
Comparison of routine field epidemiology and whole genome sequencing to identify tuberculosis transmission in a remote setting
Authors: Guthrie J. L. , Strudwick L. , Roberts B. , Allen M. , McFadzen J. , Roth D. , Jorgensen D. , Rodrigues M. , Tang P. , Hanley B. , Johnston J. , Cook V. J. , Gardy J.L. 
Published: February 2020

AbstractYukon Territory (YT) is a remote region in northern Canada with ongoing spread of tuberculosis (TB). To explore the utility of whole genome sequencing (WGS) for TB surveillance and monitoring in a setting with detailed contact tracing and interview data, we used a mixed-methods approach. Our analysis included all culture-confirmed cases in YT (2005–2014) and incorporated data from 24-locus Mycobacterial Interspersed Repetitive Units-Variable Number of Tandem Repeats (MIRU-VNTR) genotyping, WGS and contact tracing. We compared field-based (contact investigation (CI) data + MIRU-VNTR) and genomic-based (WGS + MIRU-VNTR + basic case data) investigations to identify the most likely source of each person's TB and assessed the knowledge, attitudes and practices of programme personnel around genotyping and genomics using online, multiple-choice surveys (n= 4) and an in-person group interview (n= 5). Field- and genomics-based approaches agreed for 26 of 32 (81%) cases on likely location of TB acquisition. There was less agreement in the identification of specific source cases (13/22 or 59% of cases). Single-locus MIRU-VNTR variants and limited genetic diversity complicated the analysis. Qualitative data indicated that participants viewed genomic epidemiology as a useful tool to streamline investigations, particularly in differentiating latent TB reactivation from the recent transmission. Based on this, genomic data could be used to enhance CIs, focus resources, target interventions and aid in TB programme evaluation.
The Guangzhou Twin Eye Study: 2019 Update
Authors: Ding Xiaohu , Wang Wei , Scheetz Jane , He Mingguang 
Published: February 2020

AbstractThe primary aim of the Guangzhou Twin Eye Study (GTES) is to explore the impact that genes and environmental inﬂuences have on common eye diseases. Since 2006, approximately 1300 pairs of twins, aged 7–15 years, were enrolled at baseline. Progressive phenotypes, such as cycloplegic refraction, axial length, height and weight, have been collected annually. Nonprogressive phenotypes such as parental refraction, corneal thickness, fundus photo, intraocular pressure and DNA were collected once at baseline. We are collaborating with fellow international twin researchers and psychologists to further explore links with general medical conditions. In this article, we review the history, major findings and future research directions for the GTES.
Persistence and Plasticity in Conifer Water‐Use Strategies
Authors: Berkelhammer M. , Still C. J. , Ritter F. , Winnick M. , Anderson L. , Carroll R. , Carbone M. , Williams K. H. 
Published: February 2020

AbstractThe selective use of seasonal precipitation by vegetation is critical to understanding the residence time and flow path of water in watersheds, yet there are limited datasets to test how climate alters these dynamics. Here, we use measurements of the seasonal cycle of tree ring
O for two widespread conifer species in the Rocky Mountains of North America to provide a multi‐decadal depiction of the seasonal origins of forest water use. The results show that while the conifer tree stands had a dominant preference for use of snowmelt, there were multi‐annual periods over the last four decades when use of summer precipitation was preferential. Utilization of summer rain emerged during years with increased snowfall and tree growth, suggesting that summer rain enhanced the transpiration stream only during the periods of highest water use. We hypothesize this could be explained through shallowing of the root profile during wetter periods and/or through the influence of changing water table depths on the residence time of summer precipitation in the root zone. We suggest the tree ring proxy approach used here could be applied in other watersheds to provide critical insight into the temporal dynamics of plant water use that could not be inferred from short measurement campaigns. These data on the seasonal origins of forest water are critical for understanding forest vulnerability to drought, the processes that affect precipitation pathways and residence time in watersheds and the interpretation of tree ring proxy data.
Contrasting Centennial‐Scale Climate Variability in High Mountain Asia Revealed by a Tree‐Ring Oxygen Isotope Record From Lahaul‐Spiti
Authors: Managave Shreyas , Shimla P. , Yadav Ram R. , Ramesh R. , Balakrishnan S. 
Published: February 2020

AbstractGlacier response analyses in High Mountain Asia have revealed that the glaciers from the Karakoram region were in balance or thickening while most from the other regions were thinning, the “Karakoram anomaly.” It is, however, not clear whether this contrasting glacier response in High Mountain Asia is the result of regionally varying climate trends. Here, we give evidence for regionally varying climate trends by presenting a contrasting centennial‐scale variations of oxygen isotope composition (δ18O) of juniper trees from the Indian Himalayan region (present study) and Karakoram. The persistence of contrasting δ18O trends in the two regions over the past millennium indicates that regionally contrasting climate trends are a natural phenomenon. Regionally distinct hydroclimatic trends revealed here can explain the anomalous glacier mass balance trends in the Karakoram region and hence the “Karakoram anomaly.”
Convective Bursts With Gravity Waves in Tropical Cyclones: Case Study With the Himawari‐8 Satellite and Idealized Numerical Study
Authors: Horinouchi Takeshi , Shimada Udai , Wada Akiyoshi 
Published: February 2020

AbstractConvective bursts occur frequently in tropical cyclones and help their intensification by diabatic heating, but their quantitative importance has not been established. By using the high‐frequency observation of infrared brightness temperature with Himawari‐8, a latest‐generation geostationary meteorological satellite, convective bursts in Typhoon Lan (2017) were studied. Aided with a series of numerical simulations, it was revealed that the anvil edges of many bursts are associated with finite‐amplitude gravity waves consistent with internal bores, creating warm anomalies by subsidence ahead of the edges. As the edges spread, they are thinned, and their propagation speeds are often decreased. In many such instances, gravity waves, now linear, are separated from the edges to propagate away, spreading convective heating. It is proposed that by quantifying these processes with geostationary satellites, diabatic heating by convective bursts can be estimated to diagnose their impacts on tropical‐cyclone intensification.
Polar Amplification Due to Enhanced Heat Flux Across the Halocline
Authors: Beer E. , Eisenman I. , Wagner T. J. W. 
Published: February 2020

AbstractPolar amplification is a widely discussed phenomenon, and a range of mechanisms have been proposed to contribute to it, many of which involve atmospheric and surface processes. However, substantial questions remain regarding the role of ocean heat transport. Previous studies have found that ocean heat transport into the Arctic increases under global warming, but the reasons behind this remain unresolved. Here, we investigate changes in oceanic heat fluxes and associated impacts on polar amplification using an idealized ocean‐sea ice‐climate model of the Northern Hemisphere. We show that beneath the sea ice, vertical temperature gradients across the halocline increase as the ocean warms, since the surface mixed layer temperatures in ice‐covered regions are fixed near the freezing point. These enhanced vertical temperature gradients drive enhanced horizontal heat transport into the polar region and can contribute substantially to polar amplification.
Frictional Strengths of Subduction Thrust Rocks in the Region of Shallow Slow Earthquakes
Authors: Phillips Noah John , Belzer Ben , French Melodie E. , Rowe Christie D. , Ujiie Kohtaro 
Published: February 2020

AbstractEarthquakes are expected to nucleate within velocity‐weakening materials; however, at the updip limit of the subduction seismogenic zone, the principal lithologies exhibit velocity‐strengthening behavior. At an exhumed analogue for present‐day subduction at Nankai (the Mugi Mélange, Japan) two examples of paleoseismic features occur within altered basalts, suggesting that they may be velocity‐weakening. We shear altered basalt and shale matrix from the mélange in the triaxial saw cut configuration at in situ conditions of deformation (Pc = 120 MPa, T = 150 °C) for two pore fluid factors (λ = 0.36, 0.7). The shale matrix exhibits a coefficient of friction between 0.4 and 0.5, and velocity‐strengthening behavior. Altered basalt exhibits Byerlee friction and velocity‐weakening behavior. In most experiments deformation was partitioned into Riedel shears, which have a lower percentage of clasts, clast size distributions with higher fractal dimensions, and shape factors indicating rounder clasts compared to the matrix between Riedel shears. We hypothesize that earthquakes preferentially nucleate within altered basalt at the updip limit of the seismogenic zone. More complex forms of deformation (e.g., shallow very low frequency earthquakes) may occur by mixing velocity‐weakening altered basalt into the velocity‐strengthening shale matrix. In subduction zones where the matrix is composed of shale, this complex behavior is limited to shallow depths (T < ~250 °C), above the updip transition to velocity‐weakening behavior for the matrix. Altered basalt is a ubiquitous subducting material, and future studies on its behavior through a range of subduction zone conditions are required for a full understanding of the mechanical behavior of subduction zones.
Enhanced Mid‐depth Southward Transport in the Northeast Atlantic at the Last Glacial Maximum Despite a Weaker AMOC
Authors: Menviel L. C. , Spence P. , Skinner L. C. , Tachikawa K. , Friedrich T. , Missiaen L. , Yu J. 
Published: February 2020

AbstractWhile previous studies consistently suggest that North Atlantic Deep Water (NADW) was shallower at the Last Glacial Maximum (LGM) than at pre‐industrial, its strength is still controversial. Here, using a series of LGM experiments, we show that proxy records are consistent with a shallower and
50% weaker NADW, associated with a
3° equatorward shift of the sea ice edge and convection sites in the Norwegian Sea. A shoaling and weakening of NADW further allow penetration of Antarctic Bottom Water in the North Atlantic, despite Antarctic Bottom Water transport being reduced by
40%. While the Deep Western Boundary Current in the northwest Atlantic weakens with NADW, the mid‐depth southward flow on the east side of the north Mid‐Atlantic Ridge strengthens, consistent with paleorecords. This northeast Atlantic intensification is due to a change in density gradients: a weaker AMOC reduces the transport of equatorial waters to the northeast Atlantic, thus weakening the North Atlantic zonal density gradient. The resultant globally weaker oceanic circulation at the LGM would have contributed to an increase in oceanic carbon content and thus a decrease in atmospheric CO
concentration.
Inhibition of the deubiquitinase USP10 induces degradation of SYK
Authors: Yang Jing , Meng Chengcheng , Weisberg Ellen , Case Abigail , Lamberto Ilaria , Magin Robert S. , Adamia Sophia , Wang Jinhua , Gray Nathanael , Liu Suiyang , Stone Richard , Sattler Martin , Buhrlage Sara , Griffin James D. 
Published: February 2020

AbstractBackgroundThere is growing evidence that spleen tyrosine kinase (SYK) is critical for acute myeloid leukaemia (AML) transformation and maintenance of the leukemic clone in AML patients. It has also been found to be over-expressed in AML patients, with activating mutations in foetal liver tyrosine kinase 3 (FLT3), particularly those with internal tandem duplications (FLT3-ITD), where it transactivates FLT3-ITD and confers resistance to treatment with FLT3 tyrosine kinase inhibitors (TKIs).MethodsWe have previously described a pharmacological approach to treating FLT3-ITD-positive AML that relies on proteasome-mediated FLT3 degradation via inhibition of USP10, the deubiquitinating enzyme (DUB) responsible for cleaving ubiquitin from FLT3.ResultsHere, we show that USP10 is also a major DUB required for stabilisation of SYK. We further demonstrate that degradation of SYK can be induced by USP10-targeting inhibitors. USP10 inhibition leads to death of cells driven by active SYK or oncogenic FLT3 and potentiates the anti-leukemic effects of FLT3 inhibition in these cells.ConclusionsWe suggest that USP10 inhibition is a novel approach to inhibiting SYK and impeding its role in the pathology of AML, including oncogenic FLT3-positive AML. Also, given the significant transforming role SYK in other tumours, targeting USP10 may have broader applications in cancer.
Diagnostic accuracy of circulating-free DNA for the determination of MYCN amplification status in advanced-stage neuroblastoma: a systematic review and meta-analysis
Authors: Trigg Ricky M. , Turner Suzanne D. , Shaw Jacqueline A. , Jahangiri Leila 
Published: February 2020

Abstract
Background
MYCN amplification (MNA) is the strongest indicator of poor prognosis in neuroblastoma (NB). This meta-analysis aims to determine the diagnostic accuracy of MNA analysis in circulating-free DNA (cfDNA) from advanced-stage NB patients.

Methods
A systematic review of electronic databases was conducted to identify studies exploring the detection of MNA in plasma/serum cfDNA from NB patients at diagnosis using PCR methodology. Pooled estimates for sensitivity, specificity and diagnostic odds ratio (DOR) were calculated by conducting a bivariate/HSROC random-effects meta-analysis.

Results
Seven studies, with a total of 529 advanced-stage patients, were eligible. The pooled sensitivity of cfDNA-based MNA analysis was 0.908 (95% CI, 0.818–0.956), the pooled specificity was 0.976 (0.940–0.991) and the DOR was 410.0 (−103.6 to 923.7). Sub-grouped by INSS stage, the sensitivity for stage 3 and 4 patients was 0.832 (0.677–0.921) and 0.930 (0.834–0.972), respectively. The specificity was 0.999 (0.109–1.000) and 0.974 (0.937–0.990), respectively, and the DOR was 7855.2 (−66267.0 to 81977.4) and 508.7 (−85.8 to 1103.2), respectively.

Conclusions
MNA analysis in cfDNA using PCR methodology represents a non-invasive approach to rapidly and accurately determine MNA status in patients with advanced-stage NB. Standardised methodology must be developed before this diagnostic test can enter the clinic.
Novel CUL7 biallelic mutations alter the skeletal phenotype of 3M syndrome
Authors: Takizaki Nao , Tsurusaki Yoshinori , Katsumata Kaoru , Enomoto Yumi , Murakami Hiroaki , Muroya Koji , Ishikawa Hiroshi , Aida Noriko , Nishimura Gen , Kurosawa Kenji 
Published: February 2020

Abstract3M syndrome is an autosomal recessive disorder characterized by severe growth retardation, distinct facial features, and skeletal changes, including long slender tubular bones and tall vertebral bodies. We report a Japanese patient with 3M syndrome caused by the biallelic novel variants c.1705_1708del and c.1989_1999del of CUL7. Skeletal features were consistent with 3M syndrome in the early neonatal period but became less obvious by 2 years of age.
The exotically stoichiometric compounds in Al–S system under high pressure
Authors: Shao Sen , Zhu Wenji , Lv Jian , Wang Yanchao , Chen Yue , Ma Yanming 
Published: February 2020

AbstractAluminum and sulfur, as abundant elements in earth, only form Al2S3 in nature at ambient pressure. It has been realized that the stoichiometry of compounds may change under high pressure, which is crucial in the discovery of novel materials. In this work, we systematically perform structure search for Al–S system under pressure. Four binary compounds of Al–S with exotic stoichiometries of AlS, Al2S, Al3S4, and AlS2 are found at high pressure and show exciting physical properties. In particular, Al3S4 becomes a superconductor with a predicted superconducting transition temperature Tc of 20.9 K at 100 GPa, while the pressure-induced Al2S becomes an electride, where the valence electrons of aluminum strongly localize in the interstices, acting as anions, at a pressure of 70 GPa. This work provides a viable direction for further experimental study of the properties of Al–S system.
An in vivo brain–bacteria interface: the developing brain as a key regulator of innate immunity
Authors: Herrera-Rincon Celia , Paré Jean-Francois , Martyniuk Christopher J. , Jannetty Sophia K. , Harrison Christina , Fischer Alina , Dinis Alexandre , Keshari Vishal , Novak Richard , Levin Michael 
Published: February 2020

AbstractInfections have numerous effects on the brain. However, possible roles of the brain in protecting against infection, and the developmental origin and role of brain signaling in immune response, are largely unknown. We exploited a unique Xenopus embryonic model to reveal control of innate immune response to pathogenic E. coli by the developing brain. Using survival assays, morphological analysis of innate immune cells and apoptosis, and RNA-seq, we analyzed combinations of infection, brain removal, and tail-regenerative response. Without a brain, survival of embryos injected with bacteria decreased significantly. The protective effect of the developing brain was mediated by decrease of the infection-induced damage and of apoptosis, and increase of macrophage migration, as well as suppression of the transcriptional consequences of the infection, all of which decrease susceptibility to pathogen. Functional and pharmacological assays implicated dopamine signaling in the bacteria–brain–immune crosstalk. Our data establish a model that reveals the very early brain to be a central player in innate immunity, identify the developmental origins of brain–immune interactions, and suggest several targets for immune therapies.
The promise of mRNA vaccines: a biotech and industrial perspective
Authors: Jackson Nicholas A. C. , Kester Kent E. , Casimiro Danilo , Gurunathan Sanjay , DeRosa Frank 
Published: February 2020

AbstractmRNA technologies have the potential to transform areas of medicine, including the prophylaxis of infectious diseases. The advantages for vaccines range from the acceleration of immunogen discovery to rapid response and multiple disease target manufacturing. A greater understanding of quality attributes that dictate translation efficiency, as well as a comprehensive appreciation of the importance of mRNA delivery, are influencing a new era of investment in development activities. The application of translational sciences and growing early-phase clinical experience continue to inform candidate vaccine selection. Here we review the state of the art for the prevention of infectious diseases by using mRNA and pertinent topics to the biotechnology and pharmaceutical industries.