bluuebunny/crossref_metadata_2025_split huggingface dataset:
A novel method for processing adipose-derived stromal stem cells using a closed cell washing concentration device with a hollow fiber membrane module
Authors: Hayashi Shinji , Yagi Rieko , Taniguchi Shuhei , Uji Masami , Urano Hidaka , Yoshida Shinya , Sakurai Hiroshi 
Published: January 2021

AbstractCell-assisted lipotransfer (CAL) is an advanced lipoinjection method that uses autologous lipotransfer with addition of a stromal vascular fraction (SVF) containing adipose-derived stromal stem cells (ASCs). The CAL procedure of manual isolation of cells from fat requires cell processing to be performed in clean environment. To isolate cells from fat without the need for a cell processing center, such as in a procedure in an operation theater, we developed a novel method for processing SVF using a closed cell washing concentration device (CCD) with a hollow fiber membrane module. The CCD consists of a sterilized closed circuit, bags and hollow fiber, semi-automatic device and the device allows removal of >99.97% of collagenase from SVF while maintaining sterility. The number of nucleated cells, ASCs and viability in SVF processed by this method were equivalent to those in SVF processed using conventional manual isolation. Our results suggest that the CCD system is as reliable as manual isolation and may also be useful for CAL. This approach will help in the development of regenerative medicine at clinics without a cell processing center.
Fusarium oxysporum is the pathogen responsible for stem rot of the succulent plant Echeveria ‘Perle von Nürnberg’ and observation of the infection process
Authors: Yao Jinai , Huang Peng , Chen Hanxin , Yu Deyi 
Published: January 2021

AbstractEcheveria ‘Perle von Nürnberg’ is a popular ornamental potted plant. In 2017–2018, stem rot lesions caused by Fusarium spp. were repeatedly observed on Echeveria ‘Perle von Nürnberg’ in Zhangzhou, Fujian Province, China. In this study, samples were collected to isolate the pathogen. After morphological observations, molecular identification, and pathogenicity measurements, the pathogen was confirmed as Fusarium oxysporum. Green fluorescent protein was used to label F. oxysporum to observe the pathogenic process in the host plant. Two to three days after inoculation, the pathogen conidia adhered to the surface of the host stem and germinated into hyphae. The hyphae invaded the host cortex through wounds on the plant or the stem-leaf junction. The host stem produced greyish-brown circular or irregular lesions. Five days after inoculation, the expanded hyphae invaded the vascular cylinder and proliferated. The lesions on the host stem became larger and the neighboring leaves gradually fell off. Seven days after inoculation, the thalli continued to proliferate in the host, the hyphae continued to expand, and the host pith was damaged. The lesions on the stem continued to expand; the stems and branches became withered and lodged, and numerous leaves fell off. Given the rapid infection of F. oxysporum in Echeveria ‘Perle von Nürnberg’ plants, necessary prevention and control measures should be employed prior to infection. In summary, this study demonstrated that the pathogen responsible for stem rot disease in Echeveria ‘Perle von Nürnberg’ is F. oxysporum and further revealed the invasion modes and pathways of F. oxysporum in this plant, providing an empirical basis for the diagnosis and treatment of this stem rot pathogen.
Social Media Data in an Augmented Reality System for Situation Awareness Support in Emergency Control Rooms
Authors: Fromm Jennifer , Eyilmez Kaan , Baßfeld Melina , Majchrzak Tim A. , Stieglitz Stefan 
Published: January 2021

AbstractDuring crisis situations, emergency operators require fast information access to achieve situation awareness and make the best possible decisions. Augmented reality could be used to visualize the wealth of user-generated content available on social media and enable context-adaptive functions for emergency operators. Although emergency operators agree that social media analytics will be important for their future work, it poses a challenge to filter and visualize large amounts of social media data. We conducted a goal-directed task analysis to identify the situation awareness requirements of emergency operators. By collecting tweets during two storms in Germany we evaluated the usefulness of Twitter data for achieving situation awareness and conducted interviews with emergency operators to derive filter strategies for social media data. We synthesized the results by discussing how the unique interface of augmented reality can be used to integrate social media data into emergency control rooms for situation awareness support.
Four-Field Co-evolutionary Model for Human Cognition: Variation in the Middle Stone Age/Middle Palaeolithic
Authors: Lombard Marlize , Högberg Anders 
Published: January 2021

AbstractHere we explore variation and similarities in the two best-represented population groups who lived during the Middle Stone Age and Middle Palaeolithic—the Neanderthals andHomo sapiens. Building on approaches such as gene-culture co-evolution, we propose a four-field model to discuss relationships between human cognitive evolution, biology, technology, society, and ecology. We focus on the pre-50-ka phase, because we reason that later admixing between Neanderthals andHomo sapiensin Eurasia may make it difficult to separate them in terms of cognition, or any of the other fields discussed in this paper. Using our model enabled us to highlight similarities in cognition between the two populations in terms of symbolic behaviour and social learning and to identify differences in aspects of technical and social cognition. Dissimilarities in brain-selective gene variants and brain morphology strongly suggest differences in some evolutionary trajectories that would have affected cognition. We therefore suggest that rather than insisting that Neanderthals were cognitively ‘the same’ asHomo sapiens, it may be useful to focus future studies on Neanderthal-specific cognition that may have been well-developed within their specific context at the time.
Non-linear Sorenson–Dice Exemplar Image Inpainting Based Bayes Probability for Occlusion Removal in Remote Traffic Control
Authors: Arun P. L. , Kumar R Mathusoothana S 
Published: January 2021

AbstractOcclusion removal is a significant problem to be resolved in a remote traffic control system to enhance road safety. However, the conventional techniques do not recognize traffic signs well due to the vehicles are occluded. Besides occlusion removal was not performed in existing techniques with a less amount of time. In order to overcome such limitations, Non-linear Gaussian Bilateral Filtered Sorenson–Dice Exemplar Image Inpainting Based Bayes Conditional Probability (NGBFSEII-BCP) Method is proposed. Initially, a number of remote sensing images are taken as input from Highway Traffic Dataset. Then, the NGBFSEII-BCP method applies the Non-Linear Gaussian Bilateral Filtering (NGBF) algorithm for removing the noise pixels in input images. After preprocessing, the NGBFSEII-BCP method is used to remove the occlusion in the input images. Finally, NGBFSEII-BCP Method applies Bayes conditional probability to find operation status and thereby gets higher road safety using remote sensing images. The technique conducts the simulation evaluation using metrics such as peak signal to noise ratio, computational time, and detection accuracy. The simulation result illustrates that the NGBFSEII-BCP Method increases the detection accuracy by 20% and reduces the computation time by 32% as compared to state-of-the-art works.
What Makes Genetic Discrimination Exceptional?
Authors: Hellman Deborah 
Published: January 2021

Recent advances in understanding the genetic basis of disease has inspired hope but also fear. While establishing a link between a person's genetic makeup and a propensity to disease may lead to better treatment, many scientists, physicians and genetic counselors also worry that it may lead to discrimination. Although access to health insurance is the primary concern, people also fear discrimination in life and disability insurance, employment and other contexts, such as child custody decisions or adoption. In response to this concern, many state legislatures have passed laws forbidding genetic discrimination. While most of these laws focus on health insurance, some also prohibit genetic discrimination in employment or in life or disability insurance coverage.These laws have been the subject of both praise and criticism. Defenders of the laws see them as important and necessary, though arguably incomplete. Critics view them as unjustified and unwarranted. However, the question that dominates current literature is whether genetic discrimination is meaningfully different from discrimination on the basis of general health status; or as the debate is often framed, whether anti-discrimination laws ought to be genetic or generic.
An Inquiry into Unionizing Home Healthcare Workers: Benefits for Workers and Patients
Authors: Gerrick Kristin Jenkins 
Published: January 2021

Susan Carter has not been feeling well for days. She would like to see a doctor about her chest pain and wheezing, but Susan knows that missing work will leave her client without a replacement and, worse, she could be fired. Susan is a home healthcare worker in Illinois. Like many of her fellow workers, Susan has no health insurance and cannot afford to risk losing her job by going to see a doctor.Often, Susan feels unable to handle the constant stress of her job. She helps her clients bathe and dress, prepares their meals and assists them with their medications and housekeeping. Susan travels by bus daily to care for two to five clients. She carries a pager day and night in case a client needs help with a plugged catheter or another emergency. Susan often has to work seven days a week, and she steps in to care for patients whose caregivers have left for better-paying jobs.
Health Disparities and the Law: Wrongs in Search of a Right
Authors: Bobinski Mary Anne 
Published: January 2021

Healthy People 2010 provides our Nation with the wide range of public health opportunities that exist in the first decade of the 21st century. With 467 objectives in 28 focus areas, Healthy People 2010 will be a tremendously valuable asset … . Healthy People 2010 reflects the very best in public health planning—it is comprehensive, it was created by a broad coalition of experts from many sectors, it has been designed to measure progress over time, and, most important, it clearly lays out a series of objectives to bring better health to all people in this country.The current responses to the traditional health perils … have been weakened. At the same time, it seems to this outsider as though the entire public health establishment is united around the proposition that massive public action should be taken to deal with the new “epidemics,” such as obesity and diabetes … . But the use of the term “epidemic” is just the wrong way to think about this issue. There are no noncommunicable epidemics … . Yet the designation [of] obesity as a public health epidemic is designed to signal that state coercion is appropriate … .
A Single‐Point Method to Quantitatively Diagnose the Magnetotail Flapping Motion
Authors: Rong Z. J. , Zhang C. , Klinger Lucy , Shen C. , Cui J. , Zhang Y. C. , Wei Y. 
Published: January 2021

AbstractQuantitatively estimating magnetotail flapping motion is critical for understanding and characterizing its dynamical behaviors. Such estimation can be achieved in principle by the multipoint analysis of spacecraft tetrahedron, for example, Cluster or MMS mission, but, owing to the inability of single‐point measurement to separate the spatial‐temporal variation of magnetic field, would be inadequate for a single spacecraft. Since single‐point missions dominate explorations of planetary magnetotail, we have developed a single‐point method based on the magnetic field measurement that quantitatively estimates the parameters of flapping motion, including spatial amplitude, wavelength, and propagation velocity. By comparing several applied cases with the multipoint analysis of Cluster, we demonstrate that our method can be reasonably applied to infer the average parameters over the whole flapping period when magnetotail is during quiet phase (magnetic field in magnetotail does not experience significant temporal variation). Thus, this method could be applied widely to the “big data set” accumulated by single‐point spacecraft missions in order to study magnetotail flapping dynamics.
Circularly polarised backward, broadside, and forward beam scanning cross‐slot loaded periodic planar leaky‐wave antenna
Authors: Barzegari Saeed , Forooraghi Keyvan , Arand Bijan A. 
Published: January 2021

AbstractIn the present study, a novel periodic planar leaky‐wave antenna (PLWA) was designed, simulated, and fabricated based on the substrate integrated waveguide (SIW) technology. The antenna can scan the main beam from around −60° to +20° continuously with complete suppression of open stopband (OSB) by the sweeping frequency from 9.2 to 14.1 GHz. The proposed structure was designed to have circular polarisation (CP) in the entire operating frequency range, where the broadside or centre frequency is 12.25 GHz. Antenna gain in the broadside frequency is around 13 dBi. The radiation efficiency of the structure is improved and equalised through broadside, which is around 80% in the frequency band of operation. Also, series and shunt analysis was applied to the proposed periodic PLWA in the fundamental TE10 mode. In the next step, the asymmetric was applied longitudinally and transversely to the proposed structure to achieve OSB elimination combined with circular polarised radiation simultaneously. The equivalent circuit of the proposed structure was extracted and, finally, the antenna was fabricated. Based on the results, excellent agreement was observed between the measured, modelled, and simulated results.
Establishment of First Afebrile Fit Telephone Clinic at Royal Berkshire Hospital, Reading, United Kingdom
Authors: Khistriya Arti , Aldouri Ahmed , Hagan Catherine , Hughes Sarah , Ives Tammy , Gill Rati , Banos Ines , Carey Georgina 
Published: January 2021

AbstractChildren presenting with a suspected seizure are recommended to be seen by a specialist for the diagnosis and management of the epilepsies within 2 weeks of presentation. As part of the Royal College of Pediatrics and Child Health Quality Improvement Project, our project aim was to establish a first afebrile fit telephone clinic in line with The National Institute for Health and Care Excellence guidance. Our results showed safety information was poorly provided and retained at the initial consultation and a follow-up telephone call reinforced safety information and provided a point of contact for patients and families to use. The telephone follow-up also resulted in eight direct referrals into an epilepsy clinic. It is hoped the results from this project will act as a stepping stone to setting up a consultant-led first fit clinic.
Degree of Creeping Fat Assessed by Computed Tomography Enterography is Associated with Intestinal Fibrotic Stricture in Patients with Crohn’s Disease: A Potentially Novel Mesenteric Creeping Fat Index
Authors: Li Xue-Hua , Feng Shi-Ting , Cao Qing-Hua , Coffey J Calvin , Baker Mark E , Huang Li , Fang Zhuang-Nian , Qiu Yun , Lu Bao-Lan , Chen Zhi-Hui , Li Yi , Bettenworth Dominik , Iacucci Marietta , Sun Can-Hui , Ghosh Subrata , Rieder Florian , Chen Min-Hu , Li Zi-Ping , Mao Ren 
Published: January 2021

Abstract

Background and Aims
Emerging evidence points to a link between creeping fat and the pathogenesis of Crohn’s disease [CD]. Non-invasive assessment of the severity of creeping fat on cross-sectional imaging modality has seldom been investigated. This study aimed to develop and characterize a novel mesenteric creeping fat index [MCFI] based on computed tomography [CT] in CD patients.

Methods
MCFI was developed based on vascular findings on CT in a retrospective cohort [n = 91] and validated in a prospective cohort [n = 30]. The severity of creeping fat was graded based on the extent to which mesenteric fat extended around the intestinal circumference using the vessels in the fat as a marker. The accuracy of MCFI was assessed by comparing it with the degree of creeping fat observed in surgical specimens. The relationship between MCFI and fibrostenosis was characterized by determining if these correlated. The accuracy of MCFI was compared with other radiographic indices [i.e. visceral to subcutaneous fat area ratio and fibrofatty proliferation score].

Results
In the retrospective cohort, MCFI had moderate accuracy in differentiating moderate–severe from mild fibrostenosis (area under the receiver operating characteristic [ROC] curve [AUC] = 0.799; p = 0.000). ROC analysis in the retrospective cohort identified a threshold MCFI of &gt; 3 which accurately differentiated fibrostenosis severity in the prospective cohort [AUC = 0.756; p = 0.018]. An excellent correlation was shown between MCFI and the extent of fat wrapping in specimens in the prospective cohort [r = 0.840, p = 0.000]. Neither visceral to subcutaneous fat area ratio nor fibrofatty proliferation score correlated well with the degree of intestinal fibrosis.

Conclusions
MCFI can accurately characterize the extent of mesenteric fat wrapping in surgical specimens. It may become another non-invasive measure of CD fibrostenosis.
A TEST OF SPATIAL AUTOCORRELATION ANALYSIS USING AN ISOLATION-BY-DISTANCE MODEL
Authors: Sokal Robert R , Wartenberg Daniel E 
Published: January 2021

ABSTRACT
Using the isolation-by-distance model as an example, we have examined several assumptions of spatial autocorrelation analysis applied to gene frequency surfaces. Gene frequency surfaces generated by a simulation of Wright's isolation-by-distance model were shown to exhibit spatial autocorrelation, except in the panmictic case. Identical stochastic generating processes result in surfaces with characteristics that are functions of the process parameters, such as parental vagility and neighborhood size. Differences in these parameters are detectable as differences in spatial autocorrelations after only a few generations of the simulations. Separate realizations of processes with identical parameters yield similar spatial correlograms. We have examined the inferences about population structure that could have been made from these observations if they had been real, rather than simulated, populations. From such inferences, we could have drawn conclusions about the presence of selection, migration and drift in given natural systems.
A GENETIC ANALYSIS OF THE PTERIDINE BIOSYNTHETIC ENZYME, GUANOSINE TRIPHOSPHATE CYCLOHYDROLASE, IN DROSOPHILA MELANOGASTER
Authors: Mackay William J , O'Donnell Janis M 
Published: January 2021

ABSTRACT
Strains with mutant eye color were surveyed for levels of GTP cyclohydrolase (GTP CH), the first enzyme acting in the biosynthesis of pteridines, the pigments causing red eye color in Drosophila. Six strains were found to have reduced GTP CH activity. In five of the six strains, the reduction of activity is apparent only in the adult head of homozygous mutants. We show that mutations in Punch (2-97, Pu) have severe effects on GTP CH activity. In most cases, the reduction of activity is apparent in all tissues and stages that express the enzyme. The activity of GTP CH is shown to be closely correlated with the number of Pu + genes in the genome. One ethyl methanesulfonate (EMS)-induced Pu mutant has a GTP CH enzyme that is unstable when compared with the wild-type enzyme. Mutations in Pu fall into three general classes. The largest class has a recessive lethal and eye color phenotype, 50% or higher GTP CH activity in heterozygotes, and equivalent defects in all tissues. A second class is dominant in eye color phenotype and recessive lethal, with less than 50% GTP CH activity in heterozygotes. The third class is homozygous viable and has severe reduction of activity in the adult head, but no or less severe loss in other tissues.
NONIDEAL STATISTICS AND POSITIVE CORRELATION IN PHAGE RECOMBINATION: STUDIES WITH Λ TANDEM DUPLICATION PHAGES
Authors: Hays John B , Wolf Julie B , Zagursky Robert J 
Published: January 2021

ABSTRACT
he question of nonideality in phage recombination, that is, the extent to which recombinant frequencies differ from those expected from the proportions of the two parental types in the mass culture, was addressed by experiments with λ tandem duplication phages. Isolation and genotypic analysis of triplication-phage progeny, all of which must be the result of intermolecular recombination, yielded a value of about 0.5 for the nonideality parameter h, i.e., the frequency of unlike-parent matings was only about ½ the "ideal" value. This value was independent of multiplicity and about the same for the Rec or Red recombination systems. Similar analysis of single-copy phage progeny yielded estimates of k, the ratio of intramolecular to intermolecular recombination of about ⅙ for the Rec system; no intramolecular events were detected in Red-mediated crosses. Consideration of known nonideality factors (finite input, limited number of intracellular sites for phage growth) suggests that the observed h values correspond to intracellular mixing efficiencies of 55 to 100%, depending on the number of intracellular phage growth sites assumed. Analysis of long-range positive correlation (negative interference) indicates that statistical effects caused unlike-parent double crossovers to be three to four times as frequent as an independent-event calculation would predict. In addition, Rec-mediated crosses showed a 1.3-fold positive correlation for unlike-parent crossovers (in a second interval) among the progeny of like-parent recombinations.
SPONTANEOUS MUTATIONS MODIFYING THE ACTIVITY OF ALCOHOL DEHYDROGENASE (ADH) IN DROSOPHILA MELANOGASTER
Authors: Mukai Terumi , Harada Ko , Yoshimaru Hiroshi 
Published: January 2021

ABSTRACT
In a marked-inversion balanced lethal system of the second chromosome of Drosophila melanogaster, mutations were accumulated under minimum pressure of natural selection in 1000 individual lines that originated essentially from two individuals. After about 300 generations, the specific activities of alcohol dehydrogenase of 69 randomly selected individual lines were measured with replications using four replicated vials (on 2 days—two replications per day) by observing the reduction of NAD+ to NADH at 340 nm. Total soluble protein as the basis of standardization of enzyme activity was measured by the Lowry method for each vial. A control experiment was made immediately after the establishment of 20 individual lines from a single genotype. A significant increase in genetic variance was observed among the mutation-accumulating lines but was not detected in the control experiment. The statistical analysis of the data on the basis of the one-band/one-gene hypothesis suggests that many mutations controlling the activity of alcohol dehydrogenase occurred in regions different from the alcohol dehydrogenase locus itself, mainly in the noncoding DNA. Furthermore, it is suggested that transposon-like elements are related to the induction of these changes in alcohol dehydrogenase specific activities. Additional experimental evidence supporting this conclusion is also given.