bluuebunny/crossref_metadata_2025_split huggingface dataset:
Production of phosphorus biofertilizer based on the renewable materials in large laboratory scale
Authors: Wyciszkiewicz Małgorzata , Sojka Marcin , Saeid Agnieszka 
Published: October 2019

AbstractThis paper discusses the effect of the utilization of Bacillus megaterium in the microbial solubilization process where poultry bones or ash were used as a source of renewable phosphorus. The process was performed in a large scale laboratory. The pH of the solution decreased during one-week solubilization, which had a direct influence on the increased concentration of phosphorus determined in the solution. It was proved that the phosphorus concentration in the solution was significantly correlated with the biomass concentration and pH. The trial allowed verification of the suitability of the method to prepare two P fertilizers: one based on poultry bones and one on ashes. The elemental analysis of their composition suggests that the bones are a P-bearing resource with properties better than ash. That enables for more efficient scaling-up the solubilization although the concentration of total phosphorus was comparable in both cases. The total amount (100%) of phosphorus was present in a form available to plants in the formulation based on the poultry bones, while 64% of plant available phosphorus was present in the formulation based on the ash. The concentration of Cd was significantly lower in the case of fertilizer based on bones with respect to ash.
Is Primary Care A Substitute or Complement for Other Medical Care? Evidence from Medicaid
Authors: Chen Jiajia , van den Berghe Eunkyung , Kaestner Robert 
Published: October 2019

AbstractIt is widely believed that Medicaid reimbursement for primary care is too low and that these low fees adversely affect access to healthcare for Medicaid recipients. In this article, we exploit changes in Medicaid physician fees for primary care to study the response of primary care visits and services that are complements/substitutes with primary care, including emergency department, hospitalization, prescription drugs, and imaging. Results from our study indicate that higher Medicaid fees for primary care have modest effects. Among non-blind and non-disabled adults, we find that a 25% (or $10) increase in Medicaid fees for primary care is associated with approximately a 5% of a standard deviation increase in the number of primary care visits. For the same group, we also find that the fee increase is associated with an increase in the probability of having any primary care visits of approximately 3 percentage points. For children, changes in Medicaid fees are not significantly related to the number of primary care visits. In terms of other types of care, we find some evidence that Medicaid fees for primary care are associated with prescription drug use, and no evidence that primary care fees are associated with the use of emergency department, inpatient services, or imaging. Overall, our evidence provides, at best, limited support for the large effects of Medicaid fees on service provision sometimes asserted in policy discussions.
Gas-solid Flow Behaviors in a Pressurized Multi-stage Circulating Fluidized Bed with Geldart Group B Particles
Authors: Nie Wei , Feng Rongtao , Li Junguo , Hao Zhenhua , Zhan Haijuan , Cheng Zhonghu , Fang Yitian 
Published: October 2019

Abstract
A comprehensive study of gas-solids flow behaviors was conducted in a novel multi-stage circulating fluidized bed (MCFB). Experiments were carried out in a cold model apparatus (a jetting fluidized bed, JFB, of 0.3 m diameter and 1.95 m tall, a riser of 0.15 m diameter and 12 m tall) at different elevated pressure, solids circulation rates and gas velocities. Geldart group B polystyrene particles of 400 μm in diameter and 1020 kg/m3 in density were used as bed materials. The characteristic of L-valve, axial and radial distributions of solids holdup were systematically tested at elevated pressures by pressure transducers with the frequency of 100 Hz and model PC6M of the optical fiber probes. Operating the L-valve at elevated pressure needs less cross-section average gas velocity compared to that at atmospheric pressure. Experimental results showed that under elevated pressure and high solids flow rate, the MCFB could more easily couple JFB with a riser, where the solids that entered could form three-level step-by-step supplement entrainment and multi-flow regimes formed. Besides, increasing operating pressure led to a higher the apparent solids holdup and local solids holdup. The local solids profiles behaved less uniform distribution at elevated pressure due to decreasing the gas velocity.
Synthesis and crystal structure of bis{2-bromo-6-(((4-(1-(methoxyimino)ethyl)phenyl)imino)methyl)phenolato-κ2
N,O}cobalt(II)–dichloromethane(1/1), C34H32Br2Cl4CoN4O4
Authors: Fu Tao , Ma Jin-Xia , Wei Ping , Li Qing-Lin , Zhao Ji-Xing , Zhao Li 
Published: October 2019

Abstract
C34H32Br2Cl4CoN4O4, monoclinic, C2/c (no. 15), a = 30.281(3) Å, b = 8.9971(8) Å, c = 13.9050(13) Å, β = 107.053(5)°, Z = 4, V = 3621.7(6) Å3, R
gt(F) = 0.0648, wR
ref(F
2) = 0.1629, T = 173(2) K.
Crystal structure of catena-poly[triaqua-(1,3-di(1H-imidazol-1-yl)benzene-κ2
N:N′)-(3-nitrophthalato-κ1
O)cobalt(II)] — water (2/3), C20H22N5O10.5Co
Authors: Yin Wei-Dong , Liu Qian-Long , Li Gui-Lian 
Published: October 2019

Abstract
C20H22N5O10.5Co, triclinic, P1̄ (no. 2), a = 7.9667(3) Å, b = 11.5847(4) Å, c = 13.5305(5) Å, α = 81.096(3)°, β = 83.110(3)°, γ = 69.997(4)°, V = 1156.26(8) Å3, Z = 2, R
gt(F) = 0.0439, wR
ref(F
2) = 0.1096, T = 292(1) K.
Crystal structure of 4-benzyl-1-oxo-N-phenethyl-1H-[1,4]oxazino [4,3-b]indazole-3-carboxamide, C26H21N3O3
Authors: Wei Xian , Li Ying Fen , Xu You Lin , Lv Meng Lan 
Published: October 2019

Abstract
C26H21N3O3, triclinic, P1̄ (no. 2), a = 9.149(4) Å, b = 9.249(4) Å, c = 13.508(7) Å, α = 91.995(9)°, β = 101.158(8)°, γ = 108.392(8)°, V = 1058.5(9) Å3, Z = 2, R
gt(F) = 0.0628, wR
ref(F
2) = 0.1668, T = 296(2) K.
The crystal structure of bis(imidazole-1-yl)methane monohydrate, C7H10N4O
Authors: Wang Yong , Yuan Jun , Cao Duanlin , Wang Jianlong , Chen Lizhen 
Published: October 2019

Abstract
C7H10N4O, monoclinic, I2 (no. 5), a = 15.7815(13) Å, b = 4.3242(3) Å, c = 12.4658(9) Å, β = 106.301(8)°, V = 816.50(10) Å3, Z = 4, R
gt(F) = 0.0473, wR
ref(F
2) = 0.1067, T = 113.25(10) K.
The crystal structure of bis(4-nitroimidazole-1-1yl)methane, C7H6N6O4
Authors: Yong Wang , Jun Yuan , Duanlin Cao , Jianlong Wang , Lizhen Chen 
Published: October 2019

Abstract
C7H6N6O4, monoclinic, P21/c (no. 14), a = 21.0402(7) Å, b = 7.9959(3) Å, c = 11.8012(4) Å, β = 102.379(1)°, V = 1939.22(12) Å3, Z = 8, R
gt(F) = 0.0481, wR
ref(F
2) = 0.1296, T = 170 K.
Crystal structure of a low-temperature (100 K) polymorph of catena-poly[(μ2-4,4′-bipyridine-κ2
N,N′)-bis(O,O′-diethyldithiophosphato-κ1
S)zinc(II)], C18H28N2O4P2S4Zn
Authors: Tan Yee Seng , Tiekink Edward R.T. 
Published: October 2019

Abstract
C18H28N2O4P2S4Zn, triclinic, P1̄ (no. 2), a = 15.2379(1) Å, b = 17.3322(2) Å, c = 21.8829(1) Å, α = 75.919(1)°, β = 78.050(1)°, γ = 68.170(1)°, V = 5159.97(8) Å3, Z = 8, R
gt(F) = 0.0246, wR
ref(F
2) = 0.0681, T = 100 K.
Crystal structure of dichlorido-(N′-(1-(3-ethylpyrazin-2-yl)ethylidene)-4-methoxybenzohydrazide-κ3
N,N′,O)cadmium(II), C16H18N4O2Cl2Cd
Authors: Duan Wei-Guo , Guo Yi-Peng , Lin Long , Wu Wei-Na 
Published: October 2019

Abstract
C16H18N4O2Cl2Cd, monoclinic, P21/n (no. 14), a = 9.833(2) Å, b = 17.404(4) Å, c = 11.694(3) Å, β = 112.901(3)°, V = 1843.5(8) Å3, Z = 4, R
gt(F) = 0.0280, wR
ref(F
2) = 0.0728, T = 296(2) K.
Entropy generation in an inclined porous channel with suction/injection
Authors: Jha Basant K. , Yusuf Taiwo S. 
Published: October 2019

AbstractThe present work is concerned with an analytical study of entropy generation in viscous, incompressible fluid in an inclined channel with porous walls. The solution of the governing equations were obtained in closed form. The expression for the irreversibility ratio was also obtained and the results were presented graphically and extensively discussed for different values of the dimensionless parameters. The result indicates that wall inclination enhance entropy generation.
Targeting 5α-reductase with 99mTc labeled dutasteride derivatives for prostate imaging
Authors: Gul-e-Raana  , Shah Syed Qaiser 
Published: October 2019

Abstract
To assess the suitability of 99mTc labeled 5α-reductase (5α-Rds) inhibitors for non-invasive targeting of prostate cancer (PCa) using Male Sprague Dawely Rat (MSDR) model. In this work, dutasteride (Cpd-1) a 5α-Rds inhibitor was derivatized to its dithiocarbamate analogue (Cpd-2) for subsequent synthesis of 99mTc(CO)3-dutasteride dithiocarbamate (Cpd-3) using tricarbonyl technique. To determine the structure of Cpd-3, for the first time a reference Re(CO)3-dutasteride dithiocarbamate (Cpd-4) was synthesized and characterized with NMR, ESIMS, HPLC and elemental analysis. HPLC was used to establish the identity of Cpd-3 using Cpd-4 as a reference standard. The suitability of Cpd-3 as a new 5α-Rds targeting agent was investigated, both in vitro and vivo. The Cpd-3 has shown ≥98 % in vitro stability at room temperature and was remained ≥90 % stable up to 6 h. In serum Cpd-3 has revealed an analogous behavior with a small decrease in stability after 16 h. High uptake (26.25 ± 1.10 %, after 4 h of i.v.) of Cpd-3 was observed in the prostate (target tissue) of MSDR model with reasonably good target to non-target ratio. Blocking the target site with excess Cpd-2 considerably decreased the uptake of Cpd-3 to 4.10 ± 0.75 % in PCa. High in vitro stability in saline and serum and in vivo uptake in prostate of MSDR model plausibly attracts the opportunity of using Cpd-3 as a novel radiopharmaceutical for non invasive targeting of prostate.
Validation of an analytical method to quantify serum electrolytes by atomic absorption spectroscopy
Authors: García-Alegría Alejandro Monserrat , Gómez-Álvarez Agustín , García-Rico Leticia , Serna-Félix Mercedes 
Published: October 2019

The objective of this research was to develop the procedure to validate an analytical method through the technique of spectroscopy by atomic absorption to quantify serum electrolytes. Quality parameters were used such as: accuracy, precision, repeatability, linearity, limit of detection, and limit of quantification. First some work was done in optimization of the analytical method in ideal conditions. Subsequently, some work was done with the validation of the method using a certified reference material National Institute Standard Technology (NIST) 2670a. Finally, the methodology was assessed in the determination of metals in a sample of human blood serum. The quality criteria evaluated showed consistent and acceptable values depending on the statistical test used for each parameter with a significance level of 5% (α = 0.05). The results obtained in this research can contribute to the beginning of the establishment of an official standard aimed at analysis of electrolytes (Na, K, Ca, Mg) in human blood serum so that can be used as reference by clinical laboratories, public health institutions, as well as in institutions of higher education and research centers.
Autoestima como mediador entre Afecto positivo-negativo y resiliencia en niños
Authors: González Arratia López Fuentes Norma Ivonne , Domínguez Espinosa Alejandra del Carmen , Valdez Medina José Luis 
Published: October 2019

El presente estudio tiene como objetivo verificar el rol mediador de la autoestima en la relación entre el afecto positivo (AP), afecto negativo (AN) y resiliencia en niños. Se trata de un estudio transversal con una muestra de 188 niños entre 9 y 12 años de edad, aplicándose tres escalas que incluyen medición de la resiliencia, autoestima y afecto positivo-negativo. Se realizaron análisis descriptivos, de correlación producto momento de Pearson mediante los cuales se identifican asociaciones positivas y significativas entre las variables excepto el AN. Del análisis de trayectorias se comprueba que la autoestima es una variable moderadora sobre las relaciones entre AP y resiliencia. Sin embargo, se requiere continuar identificando variables que mejor explican la resiliencia infantil.
Migración y Transformación Sociocultural: El paisaje como referente de la movilidad
Authors: Reyes Tovar Miriam , Lamy Brigitte 
Published: October 2019

La sociedad actual se caracteriza por constantes flujos de personas y grupos, quienes transportan cada uno su cultura de un lugar a otro; fenómeno que se acentúa en el mundo contemporáneo y por lo tanto, debe considerarse un tema de estudio relevante. En este sentido, es importante considerar al paisaje como unidad espacial de la Geografía Humana, que enmarca el ámbito sensible de los lugares vividos y por lo tanto evidencia las transformaciones sociales. En este sentido, el presente artículo explica la importancia de estudiar la dinámica cultural de la pertenencia en las comunidades de emigrantes y su consecuencia sobre la construcción y significación de las formas de ver, pensar y sentir los paisajes en la migración. Al final, la discusión fortalece la importancia de observar el paisaje como un referente tangible e intangible de las transformaciones culturales causadas por la movilidad, concluyendo que los paisajes pueden ser considerados como unidades espaciales para el análisis de las implicaciones culturales de la migración.
Transgenic Archaerhodopsin-3 Expression in Hypocretin/Orexin Neurons Engenders Cellular Dysfunction and Features of Type 2 Narcolepsy
Authors: Williams Rhîannan H. , Tsunematsu Tomomi , Thomas Alexia M. , Bogyo Kelsie , Yamanaka Akihiro , Kilduff Thomas S. 
Published: October 2019

Narcolepsy, characterized by excessive daytime sleepiness, is associated with dysfunction of the hypothalamic hypocretin/orexin (Hcrt) system, either due to extensive loss of Hcrt cells (Type 1, NT1) or hypothesized Hcrt signaling impairment (Type 2, NT2). Accordingly, efforts to recapitulate narcolepsy-like symptoms in mice have involved ablating these cells or interrupting Hcrt signaling. Here, we describeorexin/Archmice, in which a modified archaerhodopsin-3 gene was inserted downstream of theprepro-orexinpromoter, resulting in expression of the yellow light-sensitive Arch-3 proton pump specifically within Hcrt neurons. Histological examination along withex vivoandin vivoelectrophysiological recordings of male and femaleorexin/Archmice demonstrated silencing of Hcrt neurons when these cells were photoilluminated. However, high expression of theArchtransgene affected cellular and physiological parameters independent of photoillumination. The excitability of Hcrt neurons was reduced, and both circadian and metabolic parameters were perturbed in a subset oforexin/Archmice that exhibited high levels of Arch expression.Orexin/Archmice also had increased REM sleep under baseline conditions but did not exhibit cataplexy, a sudden loss of muscle tone during wakefulness characteristic of NT1. These aberrations resembled some aspects of mouse models with Hcrt neuron ablation, yet the number of Hcrt neurons inorexin/Archmice was not reduced. Thus,orexin/Archmice may be useful to investigate Hcrt system dysfunction when these neurons are intact, as is thought to occur in narcolepsy without cataplexy (NT2). These results also demonstrate the utility of extended phenotypic screening of transgenic models when specific neural circuits have been manipulated.SIGNIFICANCE STATEMENTOptogenetics has become an invaluable tool for functional dissection of neural circuitry. While opsin expression is often achieved by viral injection, stably integrated transgenes offer some practical advantages. Here, we demonstrate successful transgenic expression of an inhibitory opsin in hypocretin/orexin neurons, which are thought to promote or maintain wakefulness. Both brief and prolonged illumination resulted in inhibition of these neurons and induced sleep. However, even in the absence of illumination, these cells exhibited altered electrical characteristics, particularly when transgene expression was high. These aberrant properties affected metabolism and sleep, resulting in a phenotype reminiscent of the narcolepsy Type 2, a sleep disorder for which no good animal model currently exists.
Estradiol Enhances the Depolarizing Response to GABA and AMPA Synaptic Conductances in Arcuate Kisspeptin Neurons by Diminishing Voltage-Gated Potassium Currents
Authors: DeFazio R. Anthony , Navarro Marco A. , Adams Caroline E. , Milescu Lorin S. , Moenter Suzanne M. 
Published: October 2019

Synaptic and intrinsic properties interact to sculpt neuronal output. Kisspeptin neurons in the hypothalamic arcuate nucleus help convey homeostatic estradiol feedback to central systems controlling fertility. Estradiol increases membrane depolarization induced by GABAAreceptor activation in these neurons. We hypothesized that the mechanisms underlying estradiol-induced alterations in postsynaptic response to GABA, and also AMPA, receptor activation include regulation of voltage-gated potassium currents. Whole-cell recordings of arcuate kisspeptin neurons in brain slices from ovariectomized (OVX) and OVX+estradiol (OVX+E) female mice during estradiol negative feedback revealed that estradiol reduced capacitance, reduced transient and sustained potassium currents, and altered voltage dependence and kinetics of transient currents. Consistent with these observations, estradiol reduced rheobase and action potential latency. To study more directly interactions between synaptic and active intrinsic estradiol feedback targets, dynamic clamp was used to simulate GABA and AMPA conductances. Both GABA and AMPA dynamic clamp-induced postsynaptic potentials (PSPs) were smaller in neurons from OVX than OVX+E mice; blocking transient potassium currents eliminated this difference. To interrogate the role of the estradiol-induced changes in passive intrinsic properties, different Markov model structures based on the properties of the transient potassium current in cells from OVX or OVX+E mice were combinedin silicowith passive properties reflecting these two endocrine conditions. Some of tested models reproduced the effect on PSPsin silico, revealing that AMPA PSPs were more sensitive to changes in capacitance. These observations support the hypothesis that PSPs in arcuate kisspeptin neurons are regulated by estradiol-sensitive mechanisms including potassium conductances and membrane properties.SIGNIFICANCE STATEMENTKisspeptin neurons relay estradiol feedback to gonadotropin-releasing hormone neurons, which regulate the reproductive system. The fast synaptic neurotransmitters GABA and glutamate rapidly depolarize arcuate kisspeptin neurons and estradiol increases this depolarization. Estradiol reduced both potassium current in the membrane potential range typically achieved during response to fast synaptic inputs and membrane capacitance. Using simulated GABA and glutamate synaptic inputs, we showed changes in both the passive and active intrinsic properties induced byin vivoestradiol treatment affect the response to synaptic inputs, with capacitance having a greater effect on response to glutamate. The suppression of both passive and active intrinsic properties by estradiol feedback thus renders arcuate kisspeptin neurons more sensitive to fast synaptic inputs.
p75NTR and DR6 Regulate Distinct Phases of Axon Degeneration Demarcated by Spheroid Rupture
Authors: Yong Yu , Gamage Kanchana , Cheng Irene , Barford Kelly , Spano Anthony , Winckler Bettina , Deppmann Christopher 
Published: October 2019

The regressive events associated with trophic deprivation are critical for sculpting a functional nervous system. After nerve growth factor withdrawal, sympathetic axons derived from male and female neonatal mice maintain their structural integrity for ∼18 h (latent phase) followed by a rapid and near unison disassembly of axons over the next 3 h (catastrophic phase). Here we examine the molecular basis by which axons transition from latent to catastrophic phases of degeneration following trophic withdrawal. Before catastrophic degeneration, we observed an increase in intra-axonal calcium. This calcium flux is accompanied by p75 neurotrophic factor receptor-Rho-actin-dependent expansion of calcium-rich axonal spheroids that eventually rupture, releasing their contents to the extracellular space. Conditioned media derived from degenerating axons are capable of hastening transition into the catastrophic phase of degeneration. We also found that death receptor 6, but not p75 neurotrophic factor receptor, is required for transition into the catastrophic phase in response to conditioned media but not for the intra-axonal calcium flux, spheroid formation, or rupture that occur toward the end of latency. Our results support the existence of an interaxonal degenerative signal that promotes catastrophic degeneration among trophically deprived axons.SIGNIFICANCE STATEMENTDevelopmental pruning shares several morphological similarities to both disease- and injury-induced degeneration, including spheroid formation. The function and underlying mechanisms governing axonal spheroid formation, however, remain unclear. In this study, we report that axons coordinate each other's degeneration during development via axonal spheroid rupture. Before irreversible breakdown of the axon in response to trophic withdrawal, p75 neurotrophic factor receptor-RhoA signaling governs the formation and growth of spheroids. These spheroids then rupture, allowing exchange of contents ≤10 kDa between the intracellular and extracellular space to drive death receptor 6 and calpain-dependent catastrophic degeneration. This finding informs not only our understanding of regressive events during development but may also provide a rationale for designing new treatments toward myriad neurodegenerative disorders.
Expression of Heat Shock Protein 70 Is Insufficient To ExtendDrosophila melanogasterLongevity
Authors: Xiao Chengfeng , Hull Danna , Qiu Shuang , Yeung Joanna , Zheng Jie , Barwell Taylor , Robertson R Meldrum , Seroude Laurent 
Published: October 2019

AbstractIt has been known for over 20 years that Drosophila melanogaster flies with twelve additional copies of the hsp70 gene encoding the 70 kD heat shock protein lives longer after a non-lethal heat treatment. Since the heat treatment also induces the expression of additional heat shock proteins, the biological effect can be due either to HSP70 acting alone or in combination. This study used the UAS/GAL4 system to determine whether hsp70 is sufficient to affect the longevity and the resistance to thermal, oxidative or desiccation stresses of the whole organism. We observed that HSP70 expression in the nervous system or muscles has no effect on longevity or stress resistance but ubiquitous expression reduces the life span of males. We also observed that the down-regulation of hsp70 using RNAi did not affect longevity.