bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Fatal Case of COVID-19 in a Child with ALL: A Cytokine Storm and Hyperferritinemic MODS
Authors: Kamit Fulya , Malbora Baris , Atay Avni , Bayirli Derya Turan , Bektas Metin 
Published: January 2021

AbstractSince little is known about dysregulated hyperinflammatory immunological responses causing acute severe infection and multisystem inflammatory syndrome in children associated with coronavirus disease 2019 (COVID-19), the available data on therapies for severe presentations in children are very limited. Describing experiences of severe pediatric COVID-19 presentations in more detail will help improve clinical practice.In this case report, we describe the complete clinical course of a 9-year-old girl previously diagnosed with Angelman syndrome and high-risk T cell acute lymphoblastic leukemia who had been receiving reinduction chemotherapy, presented with pneumonia and acute respiratory distress syndrome, and progressively developed hyperferritinemic multiple-organ failure, a cytokine storm, and coagulopathy associated with COVID-19. She was treated with therapeutic plasma exchange, tocilizumab, hydrocortisone, and favipiravir, but she died 7 days after her admission into our pediatric intensive care unit.The utility of therapeutic plasma exchange with other immunomodulatory therapies in severe presentations requires further trials. The spectrum of the inflammatory phenotypes associated with COVID-19 should be investigated and well defined to initiate the optimal treatment strategy on time.
The Effects of Endotracheal Suctioning on Hemodynamic Parameters and Tissue Oxygenation in Pediatric Intensive Care Unit
Authors: Misirlioglu Merve , Horoz Ozden O. , Yildizdas Dinçer , Ekinci Faruk , Yontem Ahmet , Menemencioglu Ayse , Salva Gul 
Published: January 2021

AbstractAirway secretions may increase in intubated patients due to the impaired mucociliary clearance, impaired cough reflex, abnormal glottic function, insufficient moisturizing, and respiratory tract infections. As with any intervention, patients should be cautiously monitored for possible complications during the endotracheal suctioning. Procedure-related changes in the cerebral and somatic tissue oxygenation, hemodynamics, and oxygen saturation can be observed in these patients. It is important to ensure maintenance of tissue oxygenation during these and other interventions performed in critically ill children. The aim of this study was to investigate the effects of the endotracheal suctioning on tissue oxygenation in patients undergoing mechanical ventilation in the pediatric intensive care unit. Cerebral and somatic near-infrared spectroscopy (NIRS) monitoring were performed noninvasively using standardized NIRS equipment as a means of monitoring regional tissue oxygenation. Vital signs, level of sedation, pain scores, and somatic and cerebral tissue oxygenation values of mechanically ventilated patients were recorded prospectively 5 minutes before, during, and after endotracheal suctioning. Cerebral NIRS measurements did not exhibit any statistically significant changes during endotracheal suctioning. Somatic NIRS levels changed significantly before, during, and after endotracheal suctioning and remained low throughout the procedure. Endotracheal suctioning is an invasive intervention that facilitates clearance of tracheal secretions and maintenance of the oxygenation and ventilation. The maintenance of the tissue oxygenation should be documented during these and other interventions performed on critically ill children. Somatic NIRS is a useful tool for monitoring tissue oxygenation during such procedures.
Teaching About Geometric Optics: Teacher’s Notes
Authors: Nelson Jane Bray , Nelson Jim 
Published: January 2021

Teaching About Geometric Optics: Teacher’s Notes guides physics teachers to help students develop a foundational understanding of geometric optics. The cornerstone of photonics systems, geometric optics, have applications in a wide range of industries including technology, medical, and military sectors. This book covers the basics of light propagation, reflection and refraction and the use of simple optical elements such as mirrors, prisms, lenses, and optical fibers.
Key elements include:46 activities on geometric optics, covering a wide range of topicsEasy implementation, with a copy-ready student sheet and teacher notes includedReferences to appropriate Next Generation Science Standards
Physics educators and teachers in other science disciplines will fi nd this book to be an invaluable resource to teach the fundamentals. This volume contains only the notes for Teaching About Geometric Optics: Student Edition.
Capacitive and illumination systems based on printed and hybrid electronics
Authors: Peřinka Nikola , Pozo Borja , Fernández de Gorostiza Erlantz , Mendes-Felipe Cristian , Vilas-Vilela José Luis , Lanceros-Méndez Senentxu 
Published: January 2021

Abstract
Functional electronic systems have been screen- or inkjet-printed on different plastic substrates, including polyethylene terephthalate, polycarbonate and polycarbonate/acrylonitrile butadiene styrene blends. Mutual capacitive sensors were designed and printed on flexible substrates and the capacitive response and functionality of the printed sensor with integrated passive electronic components was demonstrated. The applicability of both, inkjet printing and screen printing for the development of such capacitive sensors was evaluated. The influence of the substrate, sensor design and the printing technique parameters on both printability and functionality are discussed. Further, a flexible illumination system was developed, where the printed circuit was combined with surface mounted light emitting diodes and integrated circuits. Finally, the developed capacitive sensors and the illumination system were connected to each other to demonstrate the connectivity and interoperability of the different printed circuit components.
Large gradients via correlation in random parameterized quantum circuits
Authors: Volkoff Tyler , Coles Patrick J 
Published: January 2021

Abstract
Scaling of variational quantum algorithms to large problem sizes requires efficient optimization of random parameterized quantum circuits. For such circuits with uncorrelated parameters, the presence of exponentially vanishing gradients in cost function landscapes is an obstacle to optimization by gradient descent methods. In this work, we prove that reducing the dimensionality of the parameter space by utilizing circuit modules containing spatially or temporally correlated gate layers can allow one to circumvent the vanishing gradient phenomenon. Examples are drawn from random separable circuits and asymptotically optimal variational versions of Grover’s algorithm based on the quantum alternating operator ansatz. In the latter scenario, our bounds on cost function variation imply a transition between vanishing gradients and efficient trainability as the number of layers is increased toward

O

(

2

n
/
2

)

, the optimal oracle complexity of quantum unstructured search.
Ferromagnetic gyroscopes for tests of fundamental physics
Authors: Fadeev Pavel , Timberlake Chris , Wang Tao , Vinante Andrea , Band Y B , Budker Dmitry , Sushkov Alexander O , Ulbricht Hendrik , Jackson Kimball Derek F 
Published: January 2021

Abstract
A ferromagnetic gyroscope (FG) is a ferromagnet whose angular momentum is dominated by electron spin polarization and that will process under the action of an external torque, such as that due to a magnetic field. Here we model and analyze FG dynamics and sensitivity, focusing on practical schemes for experimental realization. In the case of a freely floating FG, we model the transition from dynamics dominated by libration in relatively high externally applied magnetic fields, to those dominated by precession at relatively low applied fields. Measurement of the libration frequency enables in situ determination of the magnetic field and a technique to reduce the field below the threshold for which precession dominates the FG dynamics. We note that evidence of gyroscopic behavior is present even at magnetic fields much larger than the threshold field below which precession dominates. We also model the dynamics of an FG levitated above a type-I superconductor via the Meissner effect, and find that for FGs with dimensions larger than about 100 nm the observed precession frequency is reduced compared to that of a freely floating FG. This is due to an effect akin to negative feedback that arises from the distortion of the field from the FG by the superconductor. Finally we assess the sensitivity of an FG levitated above a type-I superconductor to exotic spin-dependent interactions under practical experimental conditions, demonstrating the potential of FGs for tests of fundamental physics.
Aquaporin-4 is a potential drug target for traumatic brain injury via aggravating the severity of brain edema
Authors: Xiong Ao , Xiong Renping , Yu Jing , Liu Yijia , Liu Ke , Jin Ge , Xu Jianzhong , Yan Jun 
Published: January 2021

Abstract

Background
Traumatic brain edema (TBE) is caused by a specific water channel mediated by membrane aquaporins. Aquaporin-4 (AQP4) plays an especially important role in this process, but the relationship between AQP4 and TBE remains unclear. The purpose of this study was to explore expression of AQP4 in the hippocampus after traumatic brain injury (TBI), as well as the effect of brain edema on skeletal protein and its function in hippocampal neurons.

Methods
The adult male Wistar rats we divided into a sham group and a TBI group, the latter of which was further divided into 1, 3, 6, 12, 24 and 72 hours (h) and 15 days (d) post injury subgroups. A proper TBI model was established, and brain edema was assessed in each group by water content. We measured the abundance of various proteins, including hypoxia inducible factor-1α (HIF-1α), AQP4, microtubule-associated protein 2 (MAP2), tau-5 protein, phosphorylated level of TAU, synaptophysin, cyclic adenosine monophosphate response element binding protein (CREB), phosphorylated CREB and general control nonrepressed 2, in each group. Hippocampal neurons and spatial memory test were analyzed in different time points.

Results
Compared with that in the sham group, the level of AQP4 in hippocampal neurons began to significantly increase at 1 h post TBI and then decreased at 15 d post TBI. During this time frame, AQP4 level peaked at 12 and 72 h, and these peaks were closely correlated with high brain water content. HIF-1α displayed a similar trend. Conversely, levels of MAP2 began to decrease at 1 h post TBI and then increase at 15 d post TBI. In addition, the most severe brain edema in rats was found at 24 h post TBI, with neuronal loss and hippocampal dendritic spine injury. Compared to those in the sham group, rats in the TBI groups had significantly prolonged latency and significantly shortened exploration time.

Conclusions
AQP4 level was closely correlated with severity of brain edema, and abnormal levels thereof aggravated such severity after TBI.
Hybrid Learning Approach Toward Situation Recognition and Handling
Authors: Rajaby Faghihi Hossein , Fazli MohammadAmin , Habibi Jafar 
Published: January 2021

Abstract
We propose a novel hybrid learning approach to gain situation awareness in smart environments by introducing a new situation identifier that combines an expert system and a machine learning approach. Traditionally, expert systems and machine learning approaches have been widely used independently to detect ongoing situations as the main functionality in smart environments in various domains. Expert systems lack the functionality to adapt the system to each user and are expensive to design based on each setting. On the other hand, machine learning approaches fail in the challenge of cold start and making explainable decisions. Using both of these approaches enables the system to use user’s feedback and capture environmental changes while exploiting the initial expert knowledge to solve the mentioned challenges. We use decision trees and situation templates as the core structure to interpret sensor data. To evaluate the proposed method, we generate a new human-annotated dataset simulating a smart environment. Our experiments show superior results compared with the initial expert system and the machine learning approach while preserving the initial expert system’s interpretability.
COMPARISON OF THE RESTRICTION ENDONUCLEASE DIGESTION PATTERNS OF MITOCHONDRIAL DNA FROM NORMAL AND MALE STERILE CYTOPLASMS OF ZEA MAYS L
Authors: Borck Kathleen S , Walbot Virginia 
Published: January 2021

ABSTRACT
High resolution gel electrophoresis has allowed the assignment of fragment number and molecular weight to EcoRI, SalI and PstI restriction fragments of mitochondrial DNA from B37 normal (N) and B37 T, C and S male sterile cytoplasmic types of maize. A minimum complexity of 450-475 kb has been established. Hybridization of cloned EcoRI fragments to restriction digests of total mitochondrial DNA suggests that at least 80% of the genome is composed of unique sequences. Restriction fragments of identical size in N, T, C and S contain similar sequence information as evidenced by their hybridization behavior.—The total SalI digest and the larger PstI fragments representing 80% of the total complexity were used to calculate the fraction of shared fragments of each pairwise combination of cytoplasmic types. The C type mtDNA is most closely allied with the other mtDNAs and shares 67% of fragments with S, 65% with N, and 60% with T. The S type mtDNA is quite divergent from N (53% shared fragments) and T (56% shared fragments). N and T share 59% of the fragments. These results are discussed in terms of the origin of mtDNA diversity in maize.
MOLECULAR CHARACTERIZATION OF RIBOSOMAL GENES ON THE Ybb- CHROMOSOME OF DROSOPHILA MELANOGASTER
Authors: Endow Sharyn A 
Published: January 2021

ABSTRACT
The question of whether the Ybb - chromosome contains ribosomal genes has been examined by using Southern blot analysis and comparing rDNA hybridization patterns for X/X and X/Ybb - DNA. The results demonstrate that the Ybb - chromosome contains sequences that hybridize to an rDNA probe under stringent conditions. Differential hybridization of some of these sequences with DNAs corresponding to different regions of a complete ribosomal gene repeat provides evidence that some of the genes on the Ybb - chromosome are type 2 repeats. Because data obtained by other workers suggest that type 2 repeats are transcribed only to low levels, these repeats may be classed as "nonfunctional". A further finding is that the ribosomal genes on the Ybb - chromosome do not undergo multiple rounds of DNA replication during polytenization of X/Ybb - cells.
MUTANTS OF PARAMECIUM DEFECTIVE IN CHEMOKINESIS TO FOLATE
Authors: DiNallo Mary C , Wohlford Mark , Van Houten Judith 
Published: January 2021

ABSTRACT
Ten mutant lines of Paramecium tetraurelia defective in attraction to folate were isolated and examined. All mutants were normal in response to other attractants and repellents tested. One mutant was able to accumulate in folate given sufficient time. All mutations were recessive and behaved as single site Mendelian lesions. Complementation tests indicate that the mutants fall into three complementation groups. Mutants of Group 2 fall into two phenotypic classes and probably represent two alleles of the mutated fol2 gene. Possible sites of the mutants' blocks in chemoresponse are discussed.
RADIATION-SENSITIVE MUTANTS OF CAENORHABDITIS ELEGANS
Authors: Hartman Philip S , Herman Robert K 
Published: January 2021

ABSTRACT
Nine rad (for abnormal radiation sensitivity) mutants hypersensitive to ultraviolet light were isolated in the small nematode Caenorhabditis elegans. The mutations are recessive to their wild-type alleles, map to four of the six linkage groups in C. elegans and define nine new games named rad-1 through rad-9. Two of the mutants—rad-1 and rad-2—are very hypersensitive to X rays, and three—rad-2, rad-3 and rad-4—are hypersensitive to methyl methanesulfonate under particular conditions of exposure. The hypersensitivity of these mutants to more than one DNA-damaging agent suggests that they may be abnormal in DNA repair. One mutant—rad-5, a temperature-sensitive sterile mutant—shows an elevated frequency of spontaneous mutation at more than one locus; rad-4, which shows a cold-sensitive embryogenesis, reduces meiotic X-chromosome nondisjunction tenfold and partially suppresses some but not all mutations that increase meiotic X-chromosome nondisjunction; the viability of rad-6 hermaphrodites is half that of rad-6 males at 25°; and newly mature (but not older) rad-8 hermaphrodites produce many inviable embryo progeny. Meiotic recombination frequencies were measured for seven rad mutants and found to be close to normal.
AN ASSESSMENT OF "HIDDEN" HETEROGENEITY WITHIN ELECTROMORPHS AT THREE ENZYME LOCI IN DEER MICE
Authors: Aquadro Charles F , Avise John C 
Published: January 2021

ABSTRACT
Allelic heterogeneity within protein electromorphs at three loci was examined in populations of deer mice (Peromyscus maniculatus) collected from five localities across North America. We used a variety of electrophoretic techniques (including several starch and acrylamide conditions, gel-sieving, and isoelectric focusing) plus heat denaturation. Of particular interest was the supernatant glutamate oxalate transaminase system (GOT-1; aspartate aminotransferase-1 of some authors), which under standard electrophoretic conditions had been shown to exhibit basically a two-allele polymorphism throughout the range of maniculatus. The use of all of the above techniques failed to uncover any additional variation for GOT-1 in these populations. Similarly, no new scorable variation was resolved at the essentially monomorphic malate dehydrogenase-1 locus by additional conditions of electrophoresis. In marked contrast to the results for the above two enzymes, the use of multiple conditions of electrophoresis resolved the 8 standard-condition electromorphs of esterase-1 into a total of 23 variants showing strong geographic differentiation in frequency. These 23 electromorphs were further divided into a total of 35 variants by thermal stability studies. However, the allelic nature of all of the thermal stability esterase variants remains to be documented. The results of this study, taken together with the remarkable geographic heterogeneity for this species in ecology, morphology, karyotype and mitochondrial DNA sequence, suggest that some form of balancing selection may be acting to maintain the GOT-1 polymorphism.
DOMINANT X-CHROMOSOME NONDISJUNCTION MUTANTS OF CAENORHABDITIS ELEGANS
Authors: Herman Robert K , Kari Claire K , Hartman Philip S 
Published: January 2021

ABSTRACT
Two classes of frameshift suppressors distributed at 22 different loci were identified in previous studies in the yeast Saccharomyces cerevisiae. These suppressors exhibited allele-specific suppression of +1 G:C insertion mutations in either glycine or proline codons, designated as group II and group III frameshift mutations, respectively. Genes corresponding to representative suppressors of each group have been shown to encode altered glycine or proline tRNAs containing four base anticodons.—This communication reports the existence of a third class of frameshift suppressor that exhibits a wider range in specificity of suppression. The suppressors map at three loci, suf12, suf13, and suf14, which are located on chromosomes IV, XV, and XIV, respectively. The phenotypes of these suppressors suggest that suppression may be mediated by genes other than those encoding the primary structure of glycine or proline tRNAs.
GENIC VARIATION IN ABUNDANT SOLUBLE PROTEINS OF DROSOPHILA MELANOGASTER AND DROSOPHILA PSEUDOOBSCURA
Authors: Singh Rama S , Coulthart Michael B 
Published: January 2021

ABSTRACT
Genic variation was surveyed for 20 proteins of Drosophila melanogaster and 18 proteins of D. pseudoobscura. Analysis was by extraction and one-dimensional polyacrylamide gel electrophoresis under nondenaturing conditions, followed by staining with Coomassie Brilliant Blue to detect soluble proteins present in relatively large amounts ("abundant soluble proteins"). D. melanogaster was polymorphic for 65% of its protein loci and an individual was heterozygous for 10% of its loci. The respective figures for D. pseudoobscura were 61% and 11%. These estimates of genic variation fall between previously published estimates obtained for these species by one-dimensional electrophoresis of soluble enzymes and those obtained by two-dimensional electrophoresis of solubilized abundant proteins. However, variation for both species could be strongly partitioned between loci, on the basis of tissue and stage expression of the proteins. The results are discussed with respect to their bearing on the possibility that abundant proteins constitute a distinct class of proteins less polymorphic than soluble enzymes.
CHROMOSOME INTERACTIONS IN DROSOPHILA MELANOGASTER. II. TOTAL FITNESS
Authors: Seager Robert D , Ayala Francisco J , Marks R William 
Published: January 2021

ABSTRACT
In a large experiment, using nearly 200 population cages, we have measured the fitness of Drosophila melanogaster homozygous (1) for the second chromosome, (2) for the third chromosome, and (3) for both chromosomes. Twentyfour second chromosomes and 24 third chromosomes sampled from a natural population were tested. The mean fitness of the homozygous flies is 0.081 ± 0.014 for the second chromosome, 0.080 ± 0.017 for the third chromosome, and 0.079 ± 0.024 for both chromosomes simultaneously. Assuming that fitnesses are multiplicative (the additive fitness model makes no sense in the present case because of the large selection coefficients involved), the expected mean fitness of the homozygotes for both chromosomes is 0.0066; their observed fitness is more than ten times greater. Thus, it appears that synergistic interactions between loci are considerable; and that, consequently, the fitness function substantially departs from linearity. Two models are tentatively suggested for the fitness function: a "threshold" model and a "synergistic" model.—The experiments reported here confirm previous results showing that the concealed genetic load present in natural populations of Drosophila is sufficient to account for the selective maintenance of numerous polymorphisms (of the order of 1000).
MARKER-DEPENDENT RECOMBINATION IN T4 BACTERIOPHAGE. I. OUTLINE OF THE PHENOMENON AND EVIDENCE SUGGESTING A MISMATCH REPAIR MECHANISM
Authors: Shcherbakov V P , Plugina L A , Kudryashova E A , Efremova O I , Sizova S T , Toompuu Oleg G 
Published: January 2021

ABSTRACT
In standard crosses, some rIIB mutants of T4 phage were found to be susceptible to an extra recombination mechanism to which the other mutants were much less susceptible. The following observations were interpreted as evidence for the mismatch-repair nature of the phenomenon: (1) Marker-dependent recombination generates exclusively double exchanges at both sides of the marker. (2) Marker-dependent recombination is highly sensitive to DNA base sequence at the site of the marker and to that at the corresponding site on the chromosome of the other parent. (3) Within certain limits, the contribution of the marker-dependent mechanism to the total recombination frequency is distance-independent and thus constitutes a constant component.
INDUCTION OF MUTATIONS BY γ-RAYS IN PREGONIAL GERM CELLS OF ZEBRAFISH EMBRYOS
Authors: Walker Charline , Streisinger George 
Published: January 2021

ABSTRACT
Specific locus (gol-1) germ line mutations are induced by γ-rays with high frequencies (about 10-5 r-1) during cleavage divisions in zebrafish. Mutant clone sizes range from 3 to 50% of the total number of germ cells, with a mean of about 10%, when embryos are exposed between the 16 and 103 cell stages. About five pregonial cells are calculated to be present during the cleavage period.