bluuebunny/crossref_metadata_2025_split huggingface dataset:
Patient Feedback on the Effectiveness of Auricular Acupuncture on Pain in Routine Clinical Care
Authors: Zeliadt Steven B. , Thomas Eva R. , Olson Juli , Coggeshall Scott , Giannitrapani Karleen , Ackland Princess E. , Reddy Kavitha P. , Federman Daniel G. , Drake David F. , Kligler Benjamin , Taylor Stephanie L. 
Published: August 2020

Objectives:
Veterans Health Administration (VHA) launched a national initiative to train providers in a specific, protocolized auricular acupuncture treatment (also called Battlefield Acupuncture or BFA) as a nonpharmacological approach to pain management. This evaluation assessed the real-world effectiveness of BFA on immediate pain relief and identified subgroups of patients for whom BFA is most effective.

Research Design:
In a cross-sectional cohort study, electronic medical record data for 11,406 Veterans treated with BFA at 57 VHA medical centers between October 2016 and September 2018 was analyzed. The multivariate analysis incorporated data on pain history, change in pain level on an 11-point scale, complications, and demographic information.

Methods:
A total of 11,406 Veterans were treated with BFA at 57 VHA medical centers between October 2016 and September 2018 and had effectiveness data recorded in their electronic medical record.

Results:
More than 3 quarters experienced immediate decreases in pain following administration of BFA, with nearly 60% reported experiencing a minimal clinically important difference in pain intensity. The average decrease in pain intensity was −2.5 points (SD=2.2) at the initial BFA treatment, and −2.2 points (SD=2.0) at subsequent treatments. BFA was effective across a wide range of Veterans with many having preexisting chronic pain, or physical, or psychological comorbid conditions. Veterans with opioid use in the year before BFA experienced less improvement, with pain intensity scores improving more among Veterans who had not recently used opioids.

Conclusion:
VHA’s rapid expansion of training providers to offer BFA as a nonpharmacological approach to pain management has benefited many Veterans.
Physical Therapy in Pediatric and Young Adult Patients With Sickle Cell Disease: Assessing Potential Benefits and Barriers
Authors: Callaway Daniel , Chawla Anjulika , Sprinz Philippa 
Published: August 2020

Background:
Despite advances in the treatment of sickle cell disease (SCD) in pediatric and young adult patients, pain remains a significant cause of disease-related morbidity. Physical therapy has been shown to be useful for the treatment of pain in young patients with chronic illnesses, however, limited data exists regarding potential benefits of physical therapy in patients with SCD.

Materials and Methods:
We conducted a web-based survey of health care providers and support staff in the New England area to identify potential benefits of and barriers to outpatient physical therapy in this patient population.

Results:
Nearly 92% of survey participants felt that physical therapy had the potential to be beneficial in pediatric and young adult patients with SCD. A majority of physicians reported having referred patients with SCD for physical therapy. Perceived potential benefits included improved functional mobility, improved chronic pain symptoms, decreased opiate use, improved mood symptoms, improved acute pain symptoms, and improved adherence with medications and clinic visits. Significant perceived barriers identified included lack of transportation, time constraints, patient lack of understanding, and difficulty with insurance coverage.

Conclusions:
Health care providers have a positive view of the use of physical therapy in the management of this patient population. Significant barriers exist which need to be addressed.
Cytoplasmic polyadenylation by TENT5A is required for proper bone formation
Authors: Gewartowska Olga , Novaliches Goretti Aranaz , Krawczyk Paweł S , Mroczek Seweryn , Kusio-Kobiałka Monika , Tarkowski Bartosz , Spoutil Frantisek , Benada Oldrich , Kofroňová Olga , Szwedziak Piotr , Cysewski Dominik , Gruchota Jakub , Szpila Marcin , Chlebowski Aleksander , Sedlacek Radislav , Prochazka Jan , Dziembowski Andrzej 
Published: August 2020

AbstractOsteoblasts orchestrate bone formation by secreting dense, highly cross-linked type I collagen and other proteins involved in osteogenesis. Mutations in Col1α1, Col1α2, or collagen biogenesis factors lead to the human genetic disease, osteogenesis imperfecta (OI). Herein, we show that the TENT5A gene, whose mutation is responsible for poorly characterized type XVIII OI, encodes an active cytoplasmic poly(A) polymerase regulating osteogenesis. TENT5A is induced during osteoblast differentiation and TENT5A KO osteoblasts are defective in mineralization. The TENT5A KO mouse recapitulates OI disease symptoms such as bone fragility and hypomineralization. Direct RNA sequencing revealed that TENT5A polyadenylates and increases expression of Col1α1 and Col1α2 RNAs, as well as those of other genes mutated in OI, resulting in lower production and improper folding of collagen chains. Thus, we have identified the specific pathomechanism of XVIII OI and report for the first time a biologically relevant post-transcriptional regulator of collagen production. We further postulate that TENT5A, possibly together with its paralogue TENT5C, is responsible for the wave of cytoplasmic polyadenylation of mRNAs encoding secreted proteins occurring during bone mineralization.
Molecular tracking devices quantify antigen distribution and archiving in the lymph node
Authors: Walsh Shannon M. , Sheridan Ryan M. , Doan Thu , Lucas Erin D. , Ware Brian C. , Fu Rui , Burchill Matthew A. , Hesselberth Jay R. , Jirón Tamburini Beth A 
Published: August 2020

ABSTRACTLive, attenuated vaccines generate humoral and cellular immune memory, increasing the duration of protective immune memory. We previously found that antigens derived from vaccination or viral infection persist within lymphatic endothelial cells (LECs) beyond the clearance of the infection, a process we termed “antigen archiving”. Technical limitations of fluorescent labeling have precluded a complete picture of antigen archiving across cell types in the lymph node. We developed a “molecular tracking device” to follow the distribution, acquisition, and retention of antigen in the lymph node. We immunized mice with an antigen conjugated to a nuclease-resistant DNA tag and used single-cell mRNA sequencing to quantify its abundance in lymph node hematopoietic and non-hematopoietic cell types. At early and late time points after vaccination we found antigen acquisition by dendritic cell populations (DCs), associated expression of genes involved in DC activation and antigen processing, and antigen acquisition and archiving by LECs as well as unexpected stromal cell types. Variable antigen levels in LECs enabled the identification of caveolar endocytosis as a mechanism of antigen acquisition or retention. Molecular tracking devices enable new approaches to study dynamic tissue dissemination of antigens and identify new mechanisms of antigen acquisition and retention at cellular resolutionin vivo.
A View of Waste Might Decrease Relaxation: The Effects of Viewing an Open Dump in a Forest Environment on the Psychological Response of Healthy Young Adults
Authors: Ernest Bielinis , Emilia Janeczko , Norimasa Takayama , Alicja Słupska , Natalia Korcz , Anna Zawadzka , Lidia Bielinis 
Published: August 2020

AbstractForest recreation can be successfully used for the psychological relaxation of respondents and can be used as a remedy for common problems with stress. The special form of forest recreation intended for restoration is forest bathing. These activities might be distracted by some factors, such as viewing buildings in the forest or using a computer in nature, which interrupt psychological relaxation. One factor that might interrupt psychological relaxation is the occurrence of an open dump in the forest during an outdoor experience. To test the hypothesis that an open dump might decrease psychological relaxation, a case study was planned that used a randomized, controlled crossover design. For this purpose, two groups of healthy young adults viewed a control forest or a forest with an open dump in reverse order and filled in psychological questionnaires after each stimulus. A pretest was used. Participants wore oblique eye patches to stop their visual stimulation before the experimental stimulation, and the physical environment was monitored. The results were analyzed using the two-way repeated measures ANOVA. The measured negative psychological indicators significantly increased after viewing the forest with waste, and the five indicators of the Profile of Mood States increased: Tension-Anxiety, Depression-Dejection, Anger-Hostility, Fatigue, and Confusion. In addition, the negative aspect of the Positive and Negative Affect Schedule increased in comparison to the control and pretest. The measured positive indicators significantly decreased after viewing the forest with waste, the positive aspect of the Positive and Negative Affect Schedule decreased, and the Restorative Outcome Scale and Subjective Vitality scores decreased (in comparison to the control and pretest). The occurrence of an open dump in the forest might interrupt a normal restorative experience in the forest by reducing psychological relaxation. Nevertheless, the mechanism of these relevancies is not known, and thus, it will be further investigated. In addition, in a future study, the size of the impact of these open dumps on normal everyday experiences should be investigated. It is proposed that different mechanisms might be responsible for these reactions; however, the aim of this manuscript is to only measure this reaction. The identified psychological reasons for these mechanisms can be assessed in further studies.
Modular HUWE1 architecture serves as hub for degradation of cell-fate decision factors
Authors: Hunkeler Moritz , Jin Cyrus Y. , Ma Michelle W. , Overwijn Daan , Monda Julie K. , Bennett Eric J. , Fischer Eric S. 
Published: August 2020

SummaryHECT ubiquitin ligases play essential roles in metazoan development and physiology. The HECT ligase HUWE1 is central to the cellular stress response by mediating degradation of key death or survival factors including Mcl1, p53, DDIT4, and Myc. As a step toward understanding regulation of HUWE1 engagement with its diverse substrates, we present here the cryo-EM structure of HUWE1, offering a first complete molecular picture of a HECT ubiquitin ligase. The ~4400 amino acid residue polypeptide forms an alpha solenoid-shaped assembly with a central pore decorated with protein interaction modules. This modularity enables HUWE1 to target a wide range of substrates for destruction. The locations of human mutations associated with severe neurodevelopmental disorders link functions of this essential enzyme with its three-dimensional organization.
Effect of the p75 Neurotrophin Receptor on Glycemic Control of Obese Mice during Time-Restricted Feeding
Authors: Podyma Brandon , Battin Katherine , Johnson Dove-Anna , Güler Ali D. , Deppmann Christopher D. 
Published: August 2020

ABSTRACTCirculating glucose regulates organismal energy homeostasis and is tightly controlled in response to feeding behavior. In overweight individuals, glucose homeostasis is often perturbed due to resistance to normal satiety signals, such as insulin and leptin, leading to type 2 diabetes and its attendant complications. An emerging dietary intervention, time-restricted feeding (TRF), aims to ameliorate the adverse metabolic consequences of obesity, however, its effectiveness on glucose control is uncertain. Here, we demonstrate that TRF is only transiently effective in reducing pre-meal serum glucose levels in obese mice lacking leptin. However, inOb/Obmice that also lack a gene known to suppress behavior associated with TRF, the p75 neurotrophin receptor (p75NTR), a sustained reduction of glucose levels is observed. These results suggest that the effectiveness of TRF on glycemic control can be enhanced with concurrent targeting of modulators of glucose homeostasis and TRF response.
Pyrethroid Resistance Intensity ofAnopheles gambiae sensu lato(Diptera: Culicidae) from Phase II Hut Trial Station in KOLOKOPE, Eastern Plateau Togo: A Potential Site to Assess the Next Generation of Long-Lasting Insecticidal Nets
Authors: Ahadji-Dabla Koffi Mensah , Chabi Joseph , Apétogbo Georges , Koffi Edoh , Hadi Melinda Patricia , Ketoh Guillaume Koffivi 
Published: August 2020

AbstractPer WHO recommendations, the implementation of the next-generation of Long Lasting Insecticidal Nets (LLINs) for malaria vector control requires appropriate investigations on the insecticide resistance profile of the vector and the impact of the LLINs on the known resistant mosquitoes. The next-generation of LLINs are actually an incorporation of a mixture of pyrethroid insecticides and a synergist such as PBO. Several studies have proven the additional impact of PBO on the increase in the mortality rate ofAnopheles gambiaes.l. (Diptera: Culicidae). However, further assessments need to be done at community level in order to set a stage for the acceleration of the WHO policies on the implementation of the next-generation of LLINs. Kolokopé is a cotton-growing area in the central region of Togo characterized by an intensive use of agro pesticides and insecticides. A phase II experimental hut station for the evaluation of mosquito control tools has been built in Kolokopé. For the characterization of the site, WHO susceptibility tests using diagnostic doses of eight insecticides, PBO synergist assay and intensity assay of three pyrethroids (5x and 10x) were conducted on adult female mosquitoes obtained from larvae collected around the site.Anopheles gambiaes.l. from Kolokopé showed high resistance to pyrethroids and DDT, but in lesser extent to carbamates and organophosphates. Likewise, high intensity of resistance to pyrethroid was observed with less than 40% mortality at 10x deltamethrin, 52 and 29% mortality at 10x permethrin and 10x alphacypermethrin, respectively. Also, the addition of PBO showed a reversal mortality which was similar to mortality rate at 10x doses of pyrethroids. The high pyrethroid intensity resistance recorded at Kolokopé could be mainly due to the pressure onAn. gambiae s.l. through the excessive use of insecticide in agriculture. This can be used for the assessment of the next-generation of LLINs either in experimental hut or at a community trial.
Genetic variation for tolerance to the downy mildew pathogenPeronospora variabilisin genetic resources of quinoa (Chenopodium quinoa)
Authors: Colque-Little Carla , Abondano Miguel Correa , Lund Ole Søgard , Amby Daniel Buchvaldt , Piepho Hans-Peter , Andreasen Christian , Schmöckel Sandra , Schmid Karl 
Published: August 2020

AbstractBackgroundQuinoa (Chenopodium quinoaWilld.) is an ancient grain crop that is tolerant to abiotic stress and has favorable nutritional properties. Downy mildew is the main disease of quinoa and is caused by infections of the biotrophic oomycetePeronospora variabilisGaüm. Since the disease causes major yield losses, identifying sources of downy mildew tolerance in genetic resources and understanding its genetic basis are important goals in quinoa breeding.ResultsWe infected 132 South American genotypes, three Danish cultivars and the weedy relativeC. albumwith a single isolate ofP. variabilisunder greenhouse conditions and observed a large variation in disease traits like severity of infection, which ranged from 5% to 83%. Linear mixed models revealed a significant effect of genotypes on disease traits with high heritabilities (0.72 to 0.81). Factors like altitude at site of origin or seed saponin content did not correlate with mildew tolerance, but stomatal width was weakly correlated with severity of infection. Despite the strong genotypic effects on mildew tolerance, genome-wide association mapping with 88 genotypes failed to identify significant marker-trait associations indicating a polygenic architecture of mildew tolerance.ConclusionsThe strong genetic effects on mildew tolerance allow to identify genetic resources, which are valuable sources of resistance in future quinoa breeding.
Holocene plant diversity dynamics shows a distinct biogeographical pattern in temperate Europe
Authors: Roleček Jan , Abraham Vojtěch , Vild Ondřej , Svobodová Helena Svitavská , Jamrichová Eva , Plesková Zuzana , Pokorný Petr , Kuneš Petr 
Published: August 2020

AbstractAimsReconstruction of the Holocene diversity changes in a biogeographically complex region. Description of major diversity patterns, testing their predictors, and their interpretation in the palaeoecological and biogeographical context. Testing the assumption that pollen record is informative with respect to plant diversity in our study area.MethodsFossil pollen extracted from 18 high-quality profiles was used as a proxy of past plant diversity. Pollen counts of tree taxa were corrected by pollen productivity, and pollen assemblages were resampled to 100 grains per sample and 150 grains per 500-years time window. SiZer analysis was used to test and visualize multi-scale diversity patterns. Linear modelling was used to identify the best predictors. SiZer maps and pollen composition were analysed using non-metric multidimensional scaling. K-means clustering and indicator species analysis were used to interpret ordination results.ResultsMean Holocene plant diversity is significantly predicted by latitude, while its temporal pattern followed the biogeographical region and elevation. Major differences were found between the Mesic and Montane Hercynia (lower diversity, increasing only in the Late Holocene) and Pannonia, the Carpathians and Warm Hercynia (higher diversity, increasing from the Early or Middle Holocene on). Low diversity in the Middle and Late Holocene is associated with the prevalence of woody and acidophilic taxa. High diversity is associated with numerous grassland and minerotrophic wetland taxa, crops and weeds. Fossil-modern pollen diversity and modern pollen-plant diversity show significant positive relationships.ConclusionsPlant diversity and its changes during the Holocene are geographically structured across temperate Europe. Main causes appear to be differences in past dynamics of the landscape openness and vegetation composition, driven mainly by changes in climate and human impact and their different timing. Fossil pollen, if appropriately treated, is a useful proxy of past plant diversity.
Tissue-specific transcription footprinting using RNA PoI DamID (RAPID) inC. elegans
Authors: Gómez-Saldivar Georgina , Osuna-Luque Jaime , Semple Jennifer I. , Glauser Dominique A. , Jarriault Sophie , Meister Peter 
Published: August 2020

AbstractDifferential gene expression across cell types underlies the development and cell physiology in multicellular organisms.C. elegansis a powerful, extensively used model to address these biological questions. A remaining bottleneck relates, however, to the difficulty to obtain comprehensive tissue-specific gene transcription data, since available methods are still challenging to execute and/or require large worm populations. Here, we introduce theRNAPoI DamID(RAPID) approach, in which the Dam methyltransferase is fused to a ubiquitous RNA polymerase subunit in order to create transcriptional footprintsviamethyl marks on the DNA of transcribed genes. To validate the method, we determined the polymerase footprints in whole animals, sorted embryonic blastomeres and in different tissues from intact young adults by driving Dam fusion expression tissue-specifically. We obtained meaningful transcriptional footprints in line with RNA-seq studies in whole animals or specific tissues. To challenge the sensitivity of RAPID and demonstrate its utility to determine novel tissue-specific transcriptional profiles, we determined the transcriptional footprints of the pair of XXX neuroendocrine cells, representing 0.2% of the somatic cell content of the animals. We identified 2362 candidate genes with putatively active transcription in XXX cells, among which the few known markers for these cells. Using transcriptional reporters for a subset of new hits, we confirmed that the majority of them were expressed in XXX and identified novel XXX-specific markers. Taken together, our work establishes RAPID as a valid method for the determination of polymerase footprints in specific tissues ofC. eleganswithout the need for cell sorting or RNA tagging.Article summaryGene expression is a major determinant of cell fate and physiology, yet it is notoriously difficult to characterize in individual cell types for the widely used model systemC. elegans. Here, we introduce a method based on thein vivocovalent modification of DNA by transcribing RNA polymerases to determine genome-wide transcription patterns in single tissues of embryos or young adult animals. We show that the method is able to identify actively transcribed genes in tissues representing down to 0.2% of the somatic cells in adult animals. Additionally, this method can be fully performed in a single laboratory by using third generation sequencing methods (ONT).
A systems based framework to computationally predict putative transcription factors and signaling pathways regulating glycan biosynthesis
Authors: Groth Theodore , Neelamegham Sriram 
Published: August 2020

AbstractGlycosylation is a common post-translational modification, and glycan biosynthesis is regulated by a set of ‘glycogenes’. The role of transcription factors (TFs) in regulating the glycogenes and related glycosylation pathways is largely unknown. This manuscript presents a multi-omics data-mining framework to computationally predict putative, tissue-specific TF regulators of glycosylation. It combines existing ChIP-Seq (Chromatin Immunoprecipitation Sequencing) and RNA-Seq data to suggest 22,519 potentially significant TF-glycogene relationships. This includes interactions involving 524 unique TFs and 341 glycogenes that span 29 TCGA (The Cancer Genome Atlas) cancer types. Here, TF-glycogene interactions appeared in clusters or ‘communities’, suggesting that changes in single TF expression during both health and disease may affect multiple carbohydrate structures. Upon applying the Fisher’s exact test along with glycogene pathway classification, we identify TFs that may specifically regulate the biosynthesis of individual glycan types. Integration with knowledge from the Reactome database provided an avenue to relate cell-signaling pathways to TFs and cellular glycosylation state. Whereas analysis results are presented for all 29 cancer types, specific focus is placed on human luminal and basal breast cancer disease progression. Overall, the computational predictions in this manuscript present a starting point for systems-wide validation of TF-glycogene relationships.