bluuebunny/crossref_metadata_2025_split huggingface dataset:
PSII-11 Effect of a feed supplement composed of native rumen microorganisms on feedlot steer digestive health
Authors: Yang Fan , Embree Jordan , Gilmore Sean , Embree Mallory M 
Published: November 2020

Abstract
Feedlot cattle that receive high grain diets are prone to developing rumen acidosis. The rumen microbiome is a critical part of feed digestion, and thus naturally occurring, native rumen microorganisms may alleviate digestive distress. A daily, in feed microbial feed supplement (MFS) (Magnius, ASCUS Biosciences Inc, San Diego, CA) containing three native rumen microbes (Chordacoccus ruminofurens, Prevotella albensis, and Succinivibrio dextrinosolvens) was evaluated. Seventy-five commercial feedlot steers (Johnson Research, LLC, Parma, Idaho) were split into three treatment groups, 25 control, 25 low-dose (3E8 cells/steer/day), and 25 high-dose (3E9 cells/steer/day). The study included three periods: acclimation (28 days), grow-out period 1 (68 days), and grow-out period 2 (73 days). The animals were individually penned for the acclimation and grow-out period 1, and were collapsed into 7–9 steers per pen for grow-out period 2. The finishing ration for grow-out period 2 was also adjusted to 89.77% concentrate (DM) from 85.56% during grow-out period 1. No significant performance differences among treatment groups were observed during grow-out period 1. In grow-out period 2, the average daily weight gain of mid-weight steers was significantly higher (P = 0.04) in animals receiving a high-dose of MFS than controls. Additionally, the rumen pH of experimental steers were significantly higher than the controls (P &lt; 0.001) during grow-out period 2. The rumen microbiome shifts further supported the observed pH differences. A strong (R2 &gt;0.6) and significant negative correlation (P &lt; 0.001) was observed between rumen pH and rumen dissolved %CO2 in all three treatment groups throughout the study. This is consistent with literature reporting that rumen CO2 accumulation may contribute significantly to rumen acidosis. These findings demonstrate the promise of using microbial based feed supplements in the improvement of feedlot cattle digestive health and performance.
PSIX-7 Grazing toxic endophyte-infected tall fescue does not influence pancreatic or small intestinal digestive enzyme activities in beef steers
Authors: Trotta Ronald J , Klotz James L , Matthews James , Swanson Kendall C 
Published: November 2020

Abstract
The objective of this experiment was to determine the influence of toxic endophyte-infected tall fescue exposure on pancreatic and small intestinal digestive enzyme activities in steers. Twelve fescue-naive beef steers [693 ± 42.7 kg of body weight (BW)] were stratified by BW and randomly assigned within BW ranks to graze non-toxic endophyte-infected tall fescue (NTE, n = 6; 0.01 ± 0.009 mg ergovaline + ergovalinine/kg) or toxic endophyte-infected tall fescue (TE, n = 6; 0.50 ± 0.048 mg ergovaline + ergovalinine/kg) pastures for 84 d. After the grazing period, steers were weighed and then slaughtered in three groups for tissue collection. A 1-m segment of the small intestine was collected 5-m distal to the pyloric sphincter. Subsamples of the small intestinal mucosa and pancreas were collected, homogenized in saline, and assayed for protein concentration. Pancreatic and small intestinal digestive enzyme activities were assayed. Data were analyzed using the GLM procedure of SAS as a randomized complete block design for effects of treatment and slaughter group (blocking factor). Steers grazing NTE pastures had a greater (P &lt; 0.001) average daily gain (0.282 vs -0.631 kg/d) and final BW at slaughter (739 vs 620 kg) than steers grazing TE pastures. Small intestinal segment mass and mucosal density (P ≥ 0.16) were not influenced by treatment. Grazing TE pasture did not influence (P ≥ 0.21) pancreatic or small intestinal protein concentrations. Pancreatic α-amylase and trypsin activity per gram pancreas (P ≥ 0.21) or per gram pancreatic protein (P ≥ 0.30) were not influenced by fescue treatment. Small intestinal glucoamylase, isomaltase, and maltase activities did not differ between steers grazing NTE or TE pastures when expressed per gram of intestinal tissue (P ≥ 0.47) or per gram of intestinal protein (P ≥ 0.60). These data indicate that decreased gains observed in cattle consuming TE are not a result of decreased pancreatic or small intestinal digestive enzyme activities.
PSIX-6 Gastrointestinal microbiome of calves fed solid diets containing different levels and sources of NDF
Authors: Virgínio Júnior Gercino F , Poczynek Milaine , Silva Ana Paula , Toledo Ariany , Cezar Amanda , Coutinho Luiz , Montenegro Horácio , Bittar Carla Maris M 
Published: November 2020

Abstract
Different levels and sources of NDF can modify the gastrointestinal microbiome. This study evaluated 18 Holstein calves housed in not-bedded suspended individual cages and fed one of three treatments: 22NDF - conventional starter containing 22% NDF (n = 7); 31NDF - starter with 31% NDF, replacing part of the corn by soybean hull (n = 6); and 22NDF+H - conventional starter with 22% NDF plus coast-cross hay ad libitum (n = 5). All animals received 4 L of milk replacer daily (24% CP; 18.5% fat; diluted to 12.5% solids), divided into two meals, being weaned at 8th week of age. After weaning, animals were housed in tropical shelters, fed with the respective solid diet and coast-cross hay ad libitum for all treatments. To evaluate the microbiome, ruminal fluid samples were collected using a modified Geishauser oral probe at weeks 2, 4, 6, 8 and 10, two hours after the morning feeding, and fecal samples were collected at birth (0) and at weeks 1, 2, 4, 8 and 10. The microbial community was determined by sequencing V3 and V4 region amplicons of the 16S rRNA gene that was amplified by PCR and sequenced by the Illumina MiSeq platform. Ruminal microbiome had no differences in diversity for the effects of weeks, treatments or interaction of both factors (Table 1). In feces, the diversity indices and evenness were higher for 22NDF+H when compared to 22NDF, with no difference for 31NDF. All indices were significantly affected by calves age. At birth, calves had the greatest diversity and richness. Week 1 and 2 had less evenness and diversity. Bacteroidota, Firmicutes_A and Firmicutes_C were the most abundant phylum in rumen and feces. The supply of hay was only effective in modifying the fecal microbiome of dairy calves, suggesting a resilience in the ruminal microbiome.
PSIX-21 Performance and health of dairy calves fed with acidified milk in tropical climates
Authors: Coelho Marina , Tomaluski Cristiane , Dondé Sophia , Toledo Ariany , Bernardes João Pedro , Jeronymo Nathalia , Virgínio Júnior Gercino F , Silva Marcos , Reis Maria Eduarda , Bittar Carla Maris M 
Published: November 2020

Abstract
The acidification of milk is used in mild climate countries as a way of conservation because it reduces the feed bacteriological load and consequently improves the animals’ performance. However, this management is hampered in hot climates. Thirty-tree males and female Holstein colostrum-fed newborn calves were used in a randomized block experimental design and fed different liquid feed: refrigerated whole milk (WM); acidified milk kept in room temperature (AM); or a commercial milk replacer (MR: 22.9% CP; 18.8% fat; 16% solids). The animals were housed in individual shelters with free access to water and starter concentrate since 2d of age. Calves were fed 6L of liquid diet divided in two meals until their gradual weaning at 56d of age. The milk was acidified to a pH of 4.5 with formic acid 12 hours prior to feeding and kept in ambient temperature (23.7°C). The pH was monitored and corrected to the target pH before each meal. Blood samples were weekly collected and health was evaluated daily. No effect on feed intake was observed (Table 1). Feeding AM decreased calves’ weight gain as compared to that for WM fed calves but with no difference from those fed MR. Fecal scores was higher for MR as compared to WM fed calves, but with no difference from AM. However, AM calves presented higher average rectal temperature than WM fed calves, but a lower number of days with fever. AM is an alternative for feeding calves in tropical climates with performance similar to that observed for MR but with lower days with fever.
PSIX-11 In vitro fermentation parameters of defatted hemp samples at different levels of irrigation
Authors: Silva Aghata , Norris Aaron , Franco Arturo , De Moura Felipe H , Fonseca Mozart 
Published: November 2020

Abstract
The objective of this study was to determine the influence of defatted hemp under three irrigation regimes (0, 100, and 200 mm: DF0, DF1, and DF2 respectively) on in vitro fermentation parameters relative to alfalfa (Medicago sativa). Fermentation kinetics was accessed through in vitro gas production technique. Two ruminally cannulated Angus steers were used as inoculum donors in four incubation runs. For each incubation, four 160-mL serum bottles containing 200 mg of air-dried samples were incubated in 14 mL of buffering media and 4 mL of rumen inoculum. Each incubation spanned 48h with gas production measured at 0, 3, 6, 12, 24, and 48-h post-incubation. In vitro true digestibility of dry matter (IVTDMD) and organic matter (IVTOMD), total gas production (TGP), metabolizable energy (ME) and total volatile fatty acids (VFA) were determined. Hemp and alfalfa were compared via orthogonal contrast using the GLIMMIX procedure of SAS (version 9.4) with treatment as fixed effect and run as a random. Relative to alfalfa (Alf) hemp displayed increased TGP (P &lt; 0.01; 31.1 vs. 27.47 mL) that remained following correction for DM and OM (P ≤ 0.05). Similarly, IVTDMD and IVTOMD were higher for hemp (P &lt; 0.01; 77.7 vs. 70.2% and 81.7 vs. 73.9%). Hemp yielded slightly higher ME (P = 0.03) but equivalent total VFA production when compared to Alf (P = 0.62), even when corrected for DM and OM. Irrigation at 200 mm decreased TGP and fermentability of fat-extracted hemp (p-value). Results also indicate that irrigation at its highest level is detrimental to the availability of ME for rumen microbes, but not with the expense of the in vitro digestibility (IVTDMD and IVTOMD) nor in total VFA produced. In conclusion, defatted hemp samples appear to have greater digestibility and ME with equivalent VFA production relative to alfalfa.
PSIX-19 Leucine supplementation alters immune responses and blood metabolites of lambs exposed to endotoxin
Authors: Birkenstock Bianca , Catey Mary , McIntosh Stacia , Lopez Francesca , Klump Logan , Ashley Ryan , Scholljegerdes Eric J , Loest Clint A 
Published: November 2020

Abstract
This study evaluated effects of supplemental Leu on immune responses and blood metabolites of 29 wether lambs (43.8±10.7 kg) exposed to lipopolysaccharide (LPS). Lambs were fed (1.8% BW daily) a diet of 70% alfalfa hay and 30% corn-based supplement in equal portions twice daily. Lambs were adapted to dietary treatments for 7 days followed by 24-hour collections. Treatments (2×3 factorial) were subcutaneous injection of saline (-LPS) or saline containing LPS to supply 3μg (+LPS) per kg BW on day 7, and daily supplementation of no Leu (NO-LEU), intravenously-infused Leu (IV-LEU), or dietary ruminally-protected Leu (RP-LEU). The IV-LEU and RP-LEU supplied L-Leu at 15mg/kg BW daily. Lambs received jugular catheters 7 days before LPS. Rectal temperature and tumor necrosis factor-α were greater for +LPS than -LPS lambs receiving NO-LEU, and not different between +LPS and -LPS lambs receiving IV-LEU and RP-LEU (LPS×LEU; P≤0.10). Cortisol was greater for +LPS than -LPS lambs 2, 4, and 8 hours after LPS (LPS×hour; P &lt; 0.01). Haptoglobin (24 hours after LPS) was greater for +LPS than -LPS lambs (P &lt; 0.01). Lymphocytes were lower for +LPS than -LPS lambs receiving NO-LEU, and not different between +LPS and -LPS lambs receiving IV-LEU and RP-LEU (LPS×LEU; P = 0.03). Serum glucose was not different at 0, 1, 2, and 4 hours, but lower for +LPS than -LPS lambs 8 hours after LPS (LPS×hour; P = 0.06). Serum Leu, isoleucine, and valine were lower for +LPS than -LPS lambs 4, 8, and 12 hours after LPS (LPS×hour; P &lt; 0.01). Serum Leu was greater for IV-LEU than NO-LEU and RP-LEU at 0 hours, not different among Leu treatments at 1, 2, 4, and 8 hours, and lower for IV-LEU and RP-LEU than NO-LEU 12 hours after LPS (LEU×hour; P &lt; 0.01). These results indicate that Leu supplementation attenuates the inflammatory response to an endotoxin in sheep.
PSVI-6 Effects of feeding a novel alfalfa leaf pellet (ProLEAF MAX™) and an alfalfa stem byproduct (ProFiber Plus™) on growth and conception rates of developing dairy heifers
Authors: Smith Laura A , Young Allen Y , Briggs Reganne K , Brady Tevan J , Reichhardt Caleb C , Thornton Kara J 
Published: November 2020

Abstract
Alfalfa is a commonly grown forage in the Intermountain west and is often included in rations for dairy animals. This study investigated the effects of including novel alfalfa products: ProLEAF MAX™ (PLM), an alfalfa leaf pellet; and ProFiber Plus™ (PFP), alfalfa stems, in the ration of dairy heifers on growth, feed efficiency, and conception. Heifers were stratified by weight and farm origin, and randomly allocated to one of three treatment groups (n = 8/treatment): control (CON, typical ration that included alfalfa hay), PLM+PFP (a ration that replaced alfalfa hay with PLM and PFP), or PFP (a ration that replaced alfalfa hay with PFP). Heifers were fed for 84 d in individual pens and feed intake and refusals were recorded daily. Weight, hip height, and wither height were recorded every two weeks and blood was collected every four weeks. Additionally, blood urea nitrogen (BUN) and conception rates were measured. Data were analyzed with day as a repeated measure and treatment as a fixed effect. Heifers fed the CON ration had increased (P &lt; 0.05) weight gain, hip height, and dry matter intake over time compared to the treatment groups. There was a treatment*time (P &lt; 0.05) effect on feed efficiency where heifers fed the PFP had more variable feed efficiency than CON or PLM+PFP. Treatment had an effect (P &gt; 0.03) on BUN where CON heifers had increased BUN compared to those fed PFP. No differences (P &lt; 0.05) were observed in conception. These data provide insight into how performance of growing heifers is affected when novel alfalfa products are included in the ration and indicate that inclusion of PFP might decrease growth of heifers by decreasing DMI. More research needs to be done to determine whether there is an optimal amount of these products to include in heifer rations.
PSVI-8 Effects of feeding ensiled or dried grape pomace on measures of nitrogen utilization in backgrounding cattle
Authors: Chibisa Gwinyai E , Ream Cody , Stevens Allison 
Published: November 2020

Abstract
Because of its content of polyphenolic compounds, feeding grape pomace (GP) could alter nitrogen (N) utilization in cattle in a manner that limits urinary N excretion and, thus, reactive N emissions. However, the preservation method used for GP, whose shelf-life is limited when fresh, could potentially cause changes in the bioactivity of the polyphenolic compounds. This study evaluated the effects of feeding either ensiled or sun-dried GP on ruminal ammonia-N (NH3-N) and plasma urea-N (PUN) concentrations, and route of N excretion in backgrounding cattle. Six ruminally-cannulated beef heifers were used in a replicated 3 × 3 Latin square design with 21-d periods. Dietary treatments were (DM basis): 1) a typical backgrounding diet (CON), 2) CON + 15% ensiled GP, and 3) CON + 15% sun-dried GP. Grape pomace partially replaced triticale silage in the diet. Ruminal fluid (d 19 at 3 h post-feeding), blood (d 21 at 3 h post-feeding), and fecal grab and spot urine samples (d 19 at 0900, 1500, and 2100; d 20 at 0300, 1200, and 1800 h; d 21 at 0000 and 0600 h) were collected and analyzed for metabolites. Data was analyzed using PROC MIXED in SAS. There was no diet effect (P ≥ 0.48) on N intake, and ruminal NH3-N and PUN concentrations. Urine N (g/d and % of N intake) and urea-N (g/d) output were lower (P &lt; 0.01) for heifers fed GP-containing diets than the CON diet. However, fecal N excretion (g/d and % of N intake) was greater (P = 0.01) for heifers fed GP-containing diets than the CON diet. In summary, although there was no diet effect ruminal NH3-N and PUN concentrations, feeding GP irrespective of preservation method, was effective in changing the route of N excretion from urine to feces, which is beneficial from an environmental standpoint.
PSXII-19 Urine Metabolomics Analysis Associated with Feed Efficiency on Crossbred Steers during the Growing and Finishing Period on Forage- and Concentrate- Based Diets
Authors: Artegoitia Virginia , Newman John , Lewis Ron , Foote Andrew P , Freetly Harvey C 
Published: November 2020

Abstract
A discovery project to identify non-invasive biomarkers that can detect subtle metabolic discrepancies for cattle feed efficiency was performed using untargeted and targeted urine metabolomics by ultra-performance liquid chromatography-mass spectrometry. Individual feed intake and body weight gain were measured in crossbred steers (n = 80) on a forage-based growing ration (stage-1) followed by a high-concentrate diet finishing ration (stage-2). Urine was collected on study days 0, 21, 42, 63, and 83 for each dietary stage. In total, 28 steers with the greatest and the least average daily-gain (ADG) within 0.32 SD of the mean of dry-matter-intake (DMI) were used. A principal component analysis of the untargeted metabolites fully segregated the highest-ADG and lowest-ADG animals, with overlap across diets (both stages). The urinary untargeted metabolites that segregated the ADG-groups (n = 199; P &lt; 0.05), included steroid-hormones, bile-acids, alpha-linolenic acid metabolites, vitamin-B6, along with products of glycine, serine and threonine metabolism (metabolic pathway analysis: impact-value &gt; 0.50; FDR &lt; 0.10). Bile acids and steroids were then quantified in urine and their associations with animal performance and carcass composition evaluated by correlation and multiple logistic regression AUC-ROC curve analyses. In stage-1, urine concentration of cortisone was associated (P &lt; 0.05) with ADG (r = -0.28), DMI (r = -0.40) and ribeye-area (r = -0.28); cortisol was associated with DMI (r = -0.32; P &lt; 0.01) and testosterone was associated with ADG (r = -0.28; P &lt; 0.01). The urine concentrations of 18 measured bile acids were negatively associated (P &lt; 0.05) with DMI, and secondary bile acids were negatively associated (P &lt; 0.01) with marbling and hot-carcass-weight. In stage-2, negative association between the bile acids glycocholic acid and deoxycholic acid with marbling and hot-carcass-weight were identified. Urine metabolomics provide new insight into the physiological mechanisms and potential biomarkers of cattle feed efficiency. USDA is an equal opportunity provider and employer.
PSXII-14 The influence of trace minerals source on copper, manganese, and zinc binding strength to rumen digesta in cattle fed a corn silage-based diet
Authors: Guimaraes Octavio , Loh Huey Yi , Thorndyke Meghan P , Tillquist Nicole M , Spears Jerry W , Engle Terry E 
Published: November 2020

Abstract
Twelve crossbred steers fitted with rumen cannulae were used to determine the influence of trace mineral (TM) source on relative binding strength of minerals in the ruminal insoluble digesta fraction (IDF). Steers (n = 4/treatment) were fed a corn silage-based diet containing no supplemental Cu, Mn, and Zn for 7d. On d 8, steers received a bolus dose (2 x NRC requirement) of the respective TM from sulfate (STM), organic (ORG; provided as Zn methionine, Mn methionine, and Cu lysine), or hydroxy TM (HTM; Micronutrients USA LLC) sources. Ruminal samples were obtained at 2 h intervals starting at -4 and ending at 24 h post dosing. Ruminal samples were centrifuged and supernatant and the IDF were collected and frozen separately. Binding strength of Cu, Mn, and Zn to the IDF was estimated at 0, 12, and 24 h using dialysis against 0.05 M Tris-EDTA. At 0 h pre-dosing, IDF concentrations of Cu, Mn, and Zn were similar across treatments. At 12 h post dosing Cu and Zn IDF concentrations were greater (P &lt; 0.05) in HTM vs. STM and ORG supplemented steers, and Mn concentrations were greater (P &lt; 0.05) in HTM and ORG vs. STM supplemented steers. At 24 h post dosing, IDF Zn concentrations were greater (P &lt; 0.05) in HTM vs STM and ORG supplemented steers and Cu and Mn concentrations were similar across treatments. At 12 and 24 h post dosing the percent Cu and Zn released following dialysis was greater (P &lt; 0.05) in HTM vs STM and ORG supplemented steers. The percent released of Cu, Mn, and Zn at 0 h, and Mn at 12 and 24 h were similar across treatments. Data indicate that a greater concentration of Cu and Zn from HTM is loosely bound to the ruminal IDF.
PSXII-7 Supplementing beef cattle finishing diets with ruminal protected live yeast affected fatty acid profiles in meat
Authors: Yang Wenzhu , He Maolong , Shen Yizhao , Ran Tao , Alzahal Ousama 
Published: November 2020

Abstract
Fatty acid (FA) composition in beef is related to beef quality and human health. It was recently reported that feeding active dried yeast (ADY) in finishing bull altered some FA of longissimus muscle. The objective was to evaluate the effects of adding ruminally protected versus non-protected ADY on FA profiles of the pars costalis diaphragmatis (PCD) muscle of beef cattle. Seventy-five Angus steers (initial BW 448 kg) were assigned to a randomized complete block design with five treatments: control (no additives), antibiotics (ANT, 330 mg monensin + 110 mg tylosin/d), ADY (1.5 g/d), encapsulated ADY (EDY, 3 g/d) and combination of ADY and EDY (MDY). The encapsulated ADY consisted of 1.5 g ADY and 2 g capsule. Antibiotics, ADY and EDY were top-dressed to a diet containing 10% barley silage and 90% barley concentrate (DM basis). The PCD samples were collected from cattle at slaughter. Intakes (g/d) of total FA (144), saturated FA (SFA; 41), monounsaturated FA (MUFA, 16) and polyunsaturated FA (PUFA, 87) were not different among treatments. Proportion of SFA (49.0%) and MUFA (48.2%) in PCD did not differ among treatments, whereas the proportion of PUFA was greater (P &lt; 0.05) with EDY (3.0%) and MDY (3.0%) than control (2.4%). The proportion of n-3 FA also tended (P &lt; 0.06) to be greater with EDY (0.49%) and MDY (0.50%) than control (0.41%) and ADY (0.41%). Steers fed EDY and MDY enhanced (P &lt; 0.05) the proportion of linoleic acid (LA n-6) and decosapentaenoic acid (DPA n-3) compared with control. These results indicated that feeding EDY and MDY increased amounts of PUFA, FA n-3, LA n-6 and DPA n-3. Inclusion of encapsulated yeasts to diet might increase the amount of bioactive yeasts reached intestines and result in better utilization of PUFA from the digesta and increase the accumulation in the intramuscular fat.