bluuebunny/crossref_metadata_2025_split huggingface dataset:
Elucidating the diversity and potential function of nonribosomal peptide and polyketide biosynthetic gene clusters in the root microbiome
Authors: Dror Barak , Wang Zongqiang , Brady Sean F. , Jurkevitch Edouard , Cytryn Eddie 
Published: June 2020

AbstractPolyketides (PKs) and nonribosomal peptides (NRPs) are two microbial secondary metabolite (SM) families known for their variety of functions, including antimicrobials, siderophores and others. Despite their involvement in bacteria-bacteria and bacteria-plant interactions, root-associated SMs are largely unexplored due to the limited cultivability of bacteria. Here, we analyzed the diversity and expression of SM-encoding biosynthetic gene clusters (BGCs) in root microbiomes by culture-independent amplicon sequencing, shotgun metagenomics and metatranscriptomics. Roots (tomato and lettuce) harbored distinct compositions of nonribosomal peptide synthetases (NRPSs) and polyketide synthases (PKSs) relative to the adjacent bulk soil, and specific BGC markers were both enriched and highly expressed in the root microbiomes. While several of the highly abundant and expressed sequences were remotely associated with known BGCs, the low similarity to characterized genes suggests their potential novelty. Low similarity genes were screened against a large set of soil-derived cosmid libraries, from which five whole BGCs of unknown function were retrieved. Three clusters were taxonomically affiliated with Actinobacteria, while the remaining were not associated with known bacteria. One Streptomyces-derived BGC was predicted to encode for a polyene with potential antifungal activity, while the others were too novel to predict chemical structure. Screening against a suite of metagenomic datasets revealed a higher abundance of retrieved clusters in roots and soil samples. In contrast, they were almost completely absent in aquatic and gut environments, supporting the notion that they might play an important role in root ecosystems. Overall, our results indicate that root microbiomes harbor a specific assemblage of undiscovered SMs.ImportanceWe identified distinct secondary metabolite (polyketide and nonribosomal peptide) encoding genes that are enriched (relative to adjacent bulk soil) and expressed in root ecosystems, yet almost completely absent in human gut and aquatic environments. Several of the genes were distantly related to genes encoding for antimicrobials and siderophores, and their high sequence variability relative to known sequences suggests that they may encode for novel metabolites and may have unique ecological functions. This study demonstrates that plant roots harbor a diverse array of unique secondary metabolite encoding genes that are highly enriched and expressed in the root ecosystem. The secondary metabolites encoded by these genes might assist the bacteria that produce them in colonization and persistence in the root environment. To explore this hypothesis, future investigations should assess their potential role in inter-bacterial and bacterial-plant interactions.
Unliganded and CMP-Neu5Ac bound structures of human α-2,6-sialyltransferase ST6Gal I at high resolution
Authors: Harrus Deborah , Harduin-Lepers Anne , Glumoff Tuomo 
Published: June 2020

ABSTRACTSialic acid residues found as terminal monosaccharides in various types of glycan chains in cell surface glycoproteins and glycolipids have been identified as important contributors of cell-cell interactions in normal vs. abnormal cellular behavior and are pivotal in diseases such as cancers. In vertebrates, sialic acids are attached to glycan chains by a conserved subset of sialyltransferases with different enzymatic and substrate specificities. ST6Gal I is a sialyltransferase using activated CMP-sialic acids as donor substrates to catalyze the formation of a α2,6-glycosidic bond between the sialic acid residue and the acceptor disaccharide LacNAc. Understanding sialyltransferases at the molecular and structural level shed light into the function. We present here two human ST6Gal I structures, which show for the first time the enzyme in the unliganded state and with the full donor substrate CMP-Neu5Ac bound. Comparison of these structures reveal flexibility of the catalytic loop, since in the unliganded structure Tyr354 adopts a conformation seen also as an alternate conformation in the substrate bound structure. CMP-Neu5Ac is bound with the side chain at C-5 of the sugar residue directed towards empty space at the surface of the protein. Furthermore, the exact binding mode of the sialic acid moiety of the substrate directly involves sialylmotifs L, S and III and positions the sialylmotif VS in the immediate vicinity.PROTEIN DATA BANK ACCESSION CODESAtomic coordinates and structure factors of the human wild-type unliganded and CMP-Neu5Ac bound ST6Gal I have been deposited with the PDB with accession codes 6QVS and 6QVT, respectively.
Single-cell transcriptomic analysis of antiviral responses and viral antagonism in Chikungunya virus-infected synovial fibroblasts
Authors: Pott Fabian , Postmus Dylan , Brown Richard J. P. , Wyler Emanuel , Neumann Elena , Landthaler Markus , Goffinet Christine 
Published: June 2020

AbstractIn recent years, (re-)emerging arboviruses including Chikungunya virus (CHIKV) and Mayaro virus (MAYV) have caused growing concern due to expansion of insect vector ranges. No protective vaccine or specific antiviral strategies are currently available. Long-term morbidity after CHIKV infection includes debilitating chronic joint pain, which has associated health and economic impact. Here, we analyzed the early cell-intrinsic response to CHIKV and MAYV infection in primary human synovial fibroblasts. This interferon-competent cell type represents a potential source of polyarthralgia induced by CHIKV infection. Synovial fibroblasts from healthy and osteoarthritic donors were similarly permissive to CHIKV and MAYV infectionex vivo. Using RNA-seq, we defined a CHIKV infection-induced transcriptional profile with several hundred interferon-stimulated and arthralgia-mediating genes upregulated. Type I interferon was both secreted by infected fibroblasts and protective when administered exogenously. IL-6 secretion, which mediates chronic synovitis, however, was not boosted by infection. Single-cell RNA-seq and flow cytometric analyses uncovered an inverse correlation of activation of innate immunity and productive infection at the level of individual cells. In summary, primary human synovial fibroblasts serve as bona-fideex vivoprimary cell model of CHIKV infection and provide a valuable platform for studies of joint tissue-associated aspects of CHIKV immunopathogenesis.
Relationship Between Blood Group and Risk of Infection and Death in COVID-19: a live Meta-Analysis
Authors: Pourali Fateme , Afshari Mahdi , Alizadeh-Navaei Reza , Javidnia Javad , Moosazadeh Mahmood , Hessami Amirhossein 
Published: June 2020

AbstractIntroductionThe relationship between ABO blood group and the incidence of COVID-19 infection and death has been investigated in several studies. The reported results were controversial, so the objective of the present study is to assess the relationship between different blood groups and the onset and mortality of COVID-19 infection using meta-analysis method.MethodsWe searched the databases using appropriate MeSH terms. We screened articles on the basis of titles, abstracts, and full texts and the articles that met the inclusion criteria were selected. Quality assessment was done with the Newcastle-Ottawa Scale checklist. The estimated frequency of COVID-19 infection and death in terms of ABO blood group and the overall estimate of the odd ratio between blood group with COVID-19 infection and death was done with 95% confidence interval.ResultsThe pooled frequency of blood groups A, B, O, and AB among COVID-19 infected individuals was estimated as 36.22%, 24.99%, 29.67%, and 9.29% respectively. The frequency of blood groups A, B, O, and AB among the dead cases due to COVID-19 infection was estimated as 40%, 23%, 29%, and 8% respectively. The odd ratio of COVID-19 infection for blood group A versus the other blood groups was estimated 1.16 (CI 95%: 1.02-1.33). The corresponding figures for blood groups O and AB versus other blood groups were estimated as 0.73 (CI 95%: 0.60-0.88) and 1.25(CI 95%: 0.84-1.86) respectively.ConclusionThis meta-analysis showed that individuals with blood group A are at higher risk for COVID-19 infection while those with blood group O are at lower risk. Although the odds ratio of death for AB blood group was non-significant, it was considerable.
Exploration role of volunteerism on the digitisation project: case of the office of the premier in Mpumalanga province, South Africa
Authors: Netshakhuma Nkholedzeni Sidney 
Published: June 2020

Purpose
The purpose of this paper is to assess the role of volunteerism during the digitisation project by the Office of the Premier in Mpumalanga with a view recommending the best practice.

Design/methodology/approach
The empirical data was collected through the semi-structured qualitative interviews with the records manager and ten volunteer archivists employed by the Office of the Premier.

Findings
Local pupils within the Mpumalanga province were recruited to participate in the digitisation project as a form of youth empowerment. The Mpumalanga Provincial Archives was not involved in the digitisation project to ensure that all digitisation specification was in line with the requirement of the provincial archives. Furthermore, a lack of resources to implement the digitisation project was cited as the main stamping block for the successful implementation of the project.

Research limitations/implications
This paper is limited to the Office of the premier, in the Mpumalanga province.

Practical implications
Archivists interested in recruiting volunteers on a digitisation project can use this paper to understand the benefits and cost of volunteer labor before putting volunteer projects into practice.

Social implications
The success of a digitisation project depends on the involvement of the Mpumalanga Provincial archives.

Originality/value
This paper presents a unique case study in South Africa of a digitisation project staffed with volunteers in the office of the Premier.
How project management practices lead to infrastructure sustainable success: an empirical study based on goal-setting theory
Authors: Liu Bingsheng , Xue Bin , Meng Junna , Chen Xingbin , Sun Ting 
Published: June 2020

PurposeThe sustainable success of infrastructures is becoming a driving force for advancing urbanization globally. However, to achieve infrastructure sustainable success (ISS), how project management practices (PMPs) play their role remains unexplored in current literature. To this end, an empirical study on whether PMPs play significant impacts on the accomplishment of ISS was conducted.Design/methodology/approachThis research is based on an empirical survey of domestic and overseas infrastructure projects managed by Chinese companies. 162 data samples were collected and further analyzed deploying structural equation modeling (SEM) on the basis of goal-setting theory. PMP factors derived from the plan-do-check-act (PDCA) ideology, i.e. culture, strategy, implementation and reflection were hypothesized and validated to have direct and indirect relationships with ISS.FindingsThe results of this study indicate that both culture and reflection can directly drive the PMPs toward ISS. Furthermore, it is revealed that by affecting mediator factors of strategy, implementation and reflection, culture is found to present an indirect influence on ISS; by affecting mediator factors of implementation and reflection, Strategy is found to present an indirect influence on ISS and by affecting the mediator factor of reflection, implementation is found to present an indirect influence on ISS.Originality/valueThese findings contribute to the body of knowledge in measuring sustainability success of project management performances by clarifying critical relationships of how PMPs lead to ISS. Prospectively, the outputs of this research will generate informative insights for practitioners to improve their PMPs in the process of pursuing ISS in future infrastructure management practices.
Linking practices to results: an analysis toward Lean Six Sigma deployment in the public sector
Authors: Juliani Fernando , de Oliveira Otávio José 
Published: June 2020

PurposeThe purpose of this paper is to present relationship between operation and management practices analyzing the results achieved by both public organizations prone to deploy Lean Six Sigma (LSS) and public organizations that have already implemented the method. The goal is to support the start of method deployment by public managers and the creation of more efficient and effective processes in public organizations.Design/methodology/approachAn analysis of synergies between findings in the multiple case study carried out in Brazilian public organizations from health and education sectors and successful implementations found in the LSS literature is made.FindingsThe fact that this research comprised organizations from different segments allowed good practices and difficulties to be identified in a unique way, adding more value to the research carried out and increasing the potential of support to the public manager. In addition, it was possible to map the most mentioned benefits of LSS deployment in both health and education sectors and link them to systematized LSS practices.Research limitations/implicationsThe presenting research should initiate a scientific discussion on public sector areas more prone to LSS and guide researchers in the development of case studies aimed at the application of this method in public organizations in general.Practical implicationsThis paper can assist public managers to become aware of the potential of the organization they manage in the adoption of LSS principles and practices enabling a better quality of services provided to citizens.Originality/valueThe finding that public health organizations are more likely to implement LSS principles and practices when compared to public organizations from other segments can guide more in-depth studies that justify why some organizations would be better able to implement the LSS method and others would face more difficulties in a hypothetical deployment.
Impacts of media richness on network features and community commitment in online games
Authors: Liao Gen-Yih , Huang Tzu-Ling , Cheng T. C. E. , Teng Ching-I 
Published: June 2020

PurposeOnline gamers form communities and offer game makers various revenue sources. Gamers' continual revenue contributions should depend on their commitment to gaming communities (CGC), where they rely heavily on communication media that carry rich information or provide enhanced media richness. However, no study has yet examined how to use the four elements of media richness to fuel gamers' CGC, revealing a research gap. Seeking to fill this gap, this study constructs a research framework and formulates hypotheses based on media richness theory (MRT).Design/methodology/approachThis study obtained responses from 1,971 online gamers and applied structural equation modeling to test the hypotheses.FindingsThis study finds that the four media richness elements have selective impacts on network convergence and interdependence that result in CGC. Moreover, compared with interdependence, network convergence has a stronger impact on CGC.Originality/valueThe findings offer online game makers insights that will enable them to design the communication functions in games to support the formation of strongly connected gamer communities. Moreover, the findings advance MRT by showing its ability to explain the formation of online gaming communities.
How to inspire customers via social media
Authors: Sheng Hong , Yang Pianpian , Feng Yuanyue 
Published: June 2020

PurposeSocial media has emerged as a new marketing channel and an important source for customer inspiration. Understanding the sources and consequences of customer inspiration via social media is important for both researchers and marketers. However, little has been done to empirically examine customer inspiration in the social media context. Specifically, the process through which customers become inspired and motivated by social media to purchase the advertised products remains elusive. Therefore, this study extends the conceptualisation of customer inspiration into the social media context, and explores the antecedents of customer inspiration from three perspectives: informational, social and personal. This study also examines the impact of customer inspiration on customers' behavioral intention.Design/methodology/approachSurvey methodology was used and data from 679 respondents were collected in China. To examine the hypothesized relationships, partial least squares (PLS) was used to analyse the data and Smart PLS 3.0 was used for data analysis.FindingsThe study findings demonstrated that the vividness of ads, social influence and promotion focus had positive impacts on the “inspired-by state”, and subsequently the “inspired-to state”, which in turn positively influenced customers' purchasing intentions. However, media richness has a negative impact on the “inspired-by state”.Originality/valueThis study provides the first evidence of customer inspiration via social media and examines its antecedents and consequences. Practically, this study offers useful guidelines and suggestions for firms on how to inspire customers using social media.
BPM perspectives to support ICSs: Exploiting the integration of formal verifications into investment service provision processes
Authors: Raucci Domenico , Santone Antonella , Mercaldo Francesco , Dyczkowski Tomasz 
Published: June 2020

PurposeThis paper investigates the criteria for a selective integration, in the multidisciplinary business process management (BPM) areas, between information technologies tools and the company's internal control systems (ICSs) aimed at directing organizational behaviours. Adopting a process-based perspective, the authors propose a formal methodology to increase ICSs aims, related to the segregation of duties (SoDs) models, efficiently and effectively.Design/methodology/approachThe authors examine the applicability of formal verifications to validate a banking process of providing investment services, which is mapped through the workflow management system. To mitigate the state explosion problem of formal methods, the authors propose an efficient methodology that has been proved on the SoDs models in the bank ICSs, as a case study.FindingsThe authors’ investigations suggest that in the BPM domain, the banking ICSs aims can benefit from the aforesaid methodologies, originating from the formal methods area, to increase the reliability and correctness in the design, modelling and implementation of the SoDs models.Originality/valueThe proposed methodology is quite general and can be efficiently applied to large-scale systems in different business contexts or areas of the BPM. Its application to the bank's SoD prevents or detects significant weaknesses, operational risks, excessive risk appetite and other undesirable behaviours in the investment services provision processes. This guarantees that the investment ordered/offered is “suitable and appropriate” with the client's risk profile, especially non-professional, required by the MiFID II Directive.
The use of eye-tracking technology to identify visualisers and verbalisers: accuracy and contributing factors
Authors: Luo Zhanni , O'Steen Billy , Brown Cheryl 
Published: June 2020

PurposeTo build adaptive learning systems for a better learning experience, designers need to identify users’ behaviour patterns and provide adaptive learning materials accordingly. This study involved a quasi-experiment and also this paper aims to investigate the accuracy of eye-tracking technology in identifying visualisers and verbalisers and the contributing factors to diverse levels of accuracy, which lays the foundation for the establishment of adaptive learning systems.Design/methodology/approachThe authors prepared eight documents with different image-text combinations with the intention of triggering participants’ natural reading habits. By analysing the eye-movement data, this author categorised the 22 participants as visualisers or verbalisers. The results were compared for accuracy measure with participants’ self-reports in response to the index of learning style questionnaire.FindingsThe results showed that visualisers and verbalisers presented significantly different eye-movement patterns, which was confirmed by the fixation data from the Tobii eye-tracker with the detection accuracy ranged from 38% to 77%. Various factors contributed to a range of levels of accuracy, including highlighted elements, learning context, complex background, low relevance of images and texts, learner differences, awareness of experimental settings, self-conception and prior knowledge.Originality/valueTo the authors’ knowledge, this is the first paper investigating the feasibility of eye-tracking technology to identify visualisers and verbalisers for the development of adaptive learning systems.
Improving the satisfaction and loyalty of BPJS healthcare in Indonesia: a Sharia perspective
Authors: Ratnawati Alifah , Mislan Cokrohadisumarto Widiyanto bin , Kholis Noor 
Published: June 2020

Purpose
The purpose of this study is to analyze how to improve the satisfaction and loyalty of Badan Penyelenggara Jaminan Sosial Kesehatan (BPJS) health patients in Indonesia based on services in Islamic hospitals, where the service quality was analyzed from a Sharīʿah perspective.

Design/methodology/approach
The sample for this study was 470 Muslim patients from BPJS health, 248 from large Islamic hospitals and 232 from small Islamic hospitals in Central Java, Indonesia. Overall, the respondents were from five large hospitals and five small hospitals. The study used eight service quality variables that were modified from the SERVQUAL, PAKSERV and CARTER models. All the variables used indicators of Sharīʿah principles and tested their effects on satisfaction and loyalty.

Findings
The results of the study showed that the insurance system has been proven to significantly increase the satisfaction and loyalty of BPJS health patients. This means that the efforts of the Indonesian Government to improve the level of health by establishing BPJS can benefit the community. Satisfaction can also be increased through the variables of reliability, empathy and responsiveness, while the loyalty of BPJS Health patients is strongly influenced by satisfaction. The sincerity variable can increase the satisfaction and loyalty of BPJS health patients in small hospitals, but not in large hospitals. On the other hand, the variable of compliance can increase the satisfaction and loyalty of BPJS health patients in large hospitals, but not in small hospitals.

Originality/value
The insurance system implemented in Indonesia can influence the improvement of satisfaction and loyalty. Also, hospitals that are fair in serving patients and that apply amanah (trust), tabligh (responsiveness) and are fast in handling BPJS health patients’ complaints are important indicators that need attention from BPJS health and hospitals. These indicators can form variables that can increase satisfaction and loyalty.