bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cytoprotective effects of combination of gypenosides andCostus pictusD.Don extract in BRIN-BD11 β-cells
Authors: Patibandla Chinmai , Campbell Mark James , Bennett Leigh Ann , Shu Xinhua , Patterson Steven 
Published: September 2020

AbstractEthnopharmacological RelevanceGypenosides andCostus pictusD.Don are used as an anti-diabetic herbal remedy in China and India respectively. However, the synergistic effect of these two extracts on β-cell protection is not yet elucidated.IntroductionIn Type 2 diabetes mellitus (T2DM), pro-inflammatory cytokines and lipotoxicity are known causes of pancreatic β-cell dysfunction and impaired insulin secretion and eventually β-cell death. Thus, any cytoprotective drug supplements can protect the β-cell and may help in T2DM treatment. Gypenosides, extracted from the Chinese medicinal herbGynostemma pentaphyllumand the leaf extract from an Indian medicinal herbCostus pictusD. Don are used in traditional medicine due to their insulin secretory properties. In our previous studies, both extracts have shown significant cytoprotective effects in insulin-secreting BRIN-BD11 cells. In the present study, we aim to investigate the synergistic effects of a combination of these extracts on BRIN-BD11 β-cell protection.MethodsCombination of extracts was prepared by adding Gypenosides withCostus pictusat 2:1 to a concentration of 18.75mg/ml. Cell viability was determined by MTT assay following treatment with combination and/or palmitate and cytokine cocktail for 24-48h. Following 24h treatment, proliferation was measured by Ki67 staining and cytoprotective gene expression was quantified by qPCR.ResultsCombination treatment of 25µg/ml enhanced cell viability both at 24h (n=8; P<0.05) and 48h (n=8; P<0.0001) treatment. Over 24h, combination treatment (25&12.5 µg/ml) showed a significant protective effect against 125µM and 250µM palmitate induced (P<0.0001) and cytokine cocktail-(TNFα 1000U, IL-1β 50U & IFNγ 1000U) (P<0.0001 & P<0.01 respectively) induced toxicity. Combination treatment over 24h increased expression of antioxidant genes Nrf2 (P<0.001), Cat (P<0.001) and Sod1 (P<0.05) along with pro-proliferative Erk1 (P<0.01) while pro-inflammatory Nfkb1 expression was reduced(P<0.001).ConclusionThe results suggest that a combination of gypenosides andcostus pictusmay protect β-cells against inflammatory cytokines and lipotoxicity caused by saturated free fatty acids associated with obesity and diabetes.
A motivational theory perspective on entrepreneurial intentions: a gender comparative study
Authors: Arshad Muhammad , Farooq Mariam , Atif Muhammad , Farooq Omer 
Published: September 2020

PurposeThis study aims to analyze individuals’ entrepreneurial intentions from the perspective of motivational theory and examines the effects of intrinsic and extrinsic factors on entrepreneurial intentions of male and female individuals.Design/methodology/approachData were collected from students graduating from Pakistan’s largest university. A structural equation modeling technique was used for model testing.FindingsIntrinsic factors such as intrinsic interest and community feeling aspiration and extrinsic factors such as perceived relative income and occupational prestige positively affect attitudes and, in turn, stimulate entrepreneurial intentions. Further, as intrinsic interest and perceived relative income scored higher among men, gender moderates those effects. Conversely, the entrepreneurial attitudes of women were primarily driven by community feeling aspiration. Notably, the positive effect of occupational prestige did not vary among men and women.Originality/valueThis paper explores the roles of intrinsic and extrinsic factors in the entrepreneurial intentions of men and women. The integration of motivational theory with gender provides insights into the determinants of entrepreneurial intentions in South Asia.
Promoting tacit knowledge application and integration through guanxi and structural holes
Authors: Liu Jiayuan , Zhu Yilin 
Published: September 2020

Purpose
This study aims to explore the impacts of structural holes (SH) and guanxi and their interaction on the application and integration of tacit knowledge among co-workers in inter-organizational arrangements.

Design/methodology/approach
The relationships were examined using a mixed-methods research approach through 305 questionnaires and 50 interviews collected from a university and a pharmaceutical company in China.

Findings
SH hinders the application and integration of tacit knowledge, while guanxi promotes these latter. In addition, guanxi moderates the negative impact of SH on tacit knowledge application (TKA) and tacit knowledge integration (TKI).

Research limitations/implications
By developing a framework to identify how co-workers strategically leverage their guanxi and SH to facilitate TKA and TKI, this study identifies the key factors that drive these latter in complex inter-organizational arrangements, contributing to the literature on knowledge management. In addition, the study makes a contribution to the advancement of SH theory by comparing guanxi with other social relationships in terms of their application to SH in different cultures, and recognizing the cultural contingencies that condition the effect of SH on knowledge management performance, thereby generating an important implication for the identification of the different roles that structural-hole owners play in brokerage situations. Finally, by comparing the difference between guanxi and interpersonal relationships in Chinese culture, this study provides a reasonable explanation for guanxi’s moderating effect on SH.

Practical implications
By uncovering the significance of guanxi cultivation with individuals who occupy yet-to-be-filled SH to make them more committed to the network, this study seeks to provide organizational leaders with helpful suggestions for the creation of incentives to fill SH.

Originality/value
By developing a framework to identify how co-workers strategically leverage their guanxi and SH to facilitate TKA and TKI, this study provides a theoretically defensible and empirically supported solution to the problems experienced by co-workers in applying and integrating tacit knowledge effectively in complex inter-organizational arrangements.
The impact of the sonic logo’s acoustic features on orienting responses, emotions and brand personality transmission
Authors: Mas Lluis , Bolls Paul , Rodero Emma , Barreda-Ángeles Miguel , Churchill Ashley 
Published: September 2020

PurposeThe purpose of this study is to determine how sonic logo’s acoustic features (intensity, pitch and pace) based on melodic tunes with no voice orient the response of consumers, attract attention, elicit levels of pleasantness and calmness and transmit brand personality traits.Design/methodology/approachA within-subject experimental factorial design is applied to measure emotional arousal (indexed as electrodermal activity) and enhancement on perceptual processing (indexed as heart rate), as well as self-reported factors, namely, calmness/excitement, pleasantness and brand personality scales.FindingsResults show a significant increase on electrodermal activity associated with fast-paced sonic logos and a decrease in heart rate in slow-paced long sonic logos. Also, fade-up, pitch-ascending fast sonic logos are defined as more exciting and descending-pitch sonic logos as more pleasant.Research limitations/implicationsThe use of sonic logos with no voice does limit its implications. Besides, the use of three variables simultaneously with 18 versions of sonic logos in a laboratory setting may have driven participants to fatigue; hence, findings should be cautiously applied.Practical implicationsFirst, sonic logos are best processed in a fade-up form. Second, fast pace is recommended to orient response, whereas slow pace is recommended to transmit calmness. Practitioners may opt for fast-paced sonic logos if the design is new or played in a noisy environment and opt for slow-paced sonic logos in already highly recognized sound designs.Originality/valueTo the best of authors’ knowledge, this study is the first to combine psychophysiological measures and self-reported scales in a laboratory experiment on how sonic logo’s acoustic features orient response, transmit emotions and personality traits.
The effect of materials and design on the reliability of through-glass vias for 2.5 D integrated circuits: a numerical study
Authors: Ahmed Omar , Okoro Chukwudi , Pollard Scott , Jiang Tengfei 
Published: September 2020

PurposeThis study aims to investigate the factors responsible for substrate cracking reliability problem in through-glass vias (TGVs), which are critical components for glass-based 2.5 D integration.Design/methodology/approachNumerical models were used to examine the driving force for substrate cracking in glass interposers due to stress coupling during heating. An analytical solution was used to demonstrate how the energy release rate (ERR) for the glass substrate cracking is affected by the via design and the mismatch in thermal strain. Then, the numerical models were implemented to investigate the design factors effects, such as the pitch distance, via diameter, via pattern, via design, effect from a stress buffer layer and the interposer materials selection on the susceptibility to substrate cracking.FindingsERR for substrate cracking was found to be directly proportional to the via diameter and the thermal mismatch strain. When a via pattern is implemented for high-density integration, a coupling in the stress fields was identified. This coupling effect was found to depend on the pitch distance, the position of the vias, and the via arrangement, suggesting a via pattern-dependent reliability behavior for glass interposers. Changing the design of the via to an annular shape or a substrate-cored via was found to be a promising approach to reduce the susceptibility to substrate cracking compared to a fully filled solid via. Also, the use of a stress buffer layer, an encouraging design prospect presented for the first time for TGVs in this study, was found to significantly reduce cracking. Finally, alternative via and substrate materials showed lower tendency for substrate cracking, indicating that the reliability of glass interposers can be further enhanced with the implementation of such new materials.Originality/valueThis study signifies the first attempt to comprehensively evaluate the susceptibility to crack formation in glass interposers during heating. Therefore, this study provides new perspectives on how to achieve a significant potential reliability improvement for TGVs.
Toward digitalization in the construction industry with immersive and drones technologies: a critical literature review
Authors: Elghaish Faris , Matarneh Sandra , Talebi Saeed , Kagioglou Michail , Hosseini M. Reza , Abrishami Sepehr 
Published: September 2020

PurposeIn this study, a critical literature review was utilized in order to provide a clear review of the relevant existing studies. The literature was analyzed using the meta-synthesis technique to evaluate and integrate the findings in a single context.Design/methodology/approachDigital transformation in construction requires employing a wide range of various technologies. There is significant progress of research in adopting technologies such as unmanned aerial vehicles (UAVs), also known as drones, and immersive technologies in the construction industry over the last two decades. The purpose of this research is to assess the current status of employing UAVs and immersive technologies toward digitalizing the construction industry and highlighting the potential applications of these technologies, either individually or in combination and integration with each other.FindingsThe key findings are: (1) UAVs in conjunction with 4D building information modeling (BIM) can be used to assess the project progress and compliance checking of geometric design models, (2) immersive technologies can be used to enable controlling construction projects remotely, applying/checking end users’ requirements, construction education and team collaboration.Practical implicationsA detailed discussion around the application of UAVs and immersive technologies is provided. This is expected to support gaining an in-depth understanding of the practical applications of these technologies in the industry.Originality/valueThe review contributes a needed common basis for capturing progress made in UAVs and immersive technologies to date and assessing their impact on construction projects. Moreover, this paper opens a new horizon for novice researchers who will conduct research toward digitalized construction.
Behavioural and emotional disturbances associated with sleep‐disordered breathing symptomatology in children with Down's syndrome
Authors: McConnell E. J. , Hill E. A. , Celmiņa M. , Kotoulas S. ‐ C. , Riha R. L. 
Published: September 2020

AbstractBackgroundDown's syndrome (DS) predisposes to sleep‐disordered breathing (SDB). In children with DS, behavioural and emotional disturbances secondary to SDB are often assumed to result from cognitive impairment alone. Our aim was to explore the relationship of behavioural and emotional disturbances with SDB in a population of children with DS.MethodsA modified sleep questionnaire, Epworth Sleepiness Scale (ESS), Paediatric ESS and the short form of the developmental behaviour checklist (DBC‐P24) were sent to 261 carers of children aged 4 to 15 years with DS in 2012.ResultsOf 120 participants, 25% had probable SDB. In children with probable SDB compared to those without nocturnal symptoms, the total behaviour problem score (TBPS) was significantly higher (20.3 ± 8.6/48 vs. 12 ± 7.5/48; P = 0.002) as was the PaedESS (7.7 ± 5.6/24 vs. 2.8 ± 3.5/24; P = 0.002). For every increase in frequency of choking attacks, snoring and night awakenings, the TBPS increased by 1.37, 1.28 and 1.75 points, respectively, indicating worsening behaviour. The TBPS was found to decrease by 1.31 points for every hour more of self‐reported sleep duration (r = −0.25, P = 0.017).ConclusionsSDB symptoms and shorter self‐reported sleep duration are highly prevalent among children with DS and are independently associated with worsening behaviour using the TBPS.
Eleven Years’ Monitoring of the World’s Most Successful Male Biathlete of the Last Decade
Authors: Schmitt Laurent , Bouthiaux Stéphane , Millet Grégoire P. 
Published: September 2020

Purpose: To report the changes in the training characteristics, performance, and heart-rate variability (HRV) of the world’s most successful male biathlete of the last decade. Method: During the analyzed 11-year (2009–2019) period, the participant won 7 big crystal globes, corresponding to the winner of the International Biathlon Union World Cup. The training characteristics are reported as yearly volume (in hours) of low-intensity training (LIT), moderate- and high-intensity training, and speed and strength training. Performance was quantified by the number of World Cup top-3 positions per season. HRV was expressed as low- and high-frequency spectral power (in milliseconds squared), root-mean-square difference of successive R–R interval (in milliseconds), and heart rate (in beats per minute). Results: The training volume increased from 530 to ∼700 hours per year in 2009–2019, with a large polarization in training intensity distribution (ie, LIT 86.3% [2.9%]; moderate-intensity training 3.4% [1.5%]; high-intensity training 4.0% [0.7%]; strength 6.3% [1.6%]). The number of top-3 positions increased from 2 to 24–26 in 2009–2018 but decreased to 6 in 2019. The mean supine values in the root-mean-square difference of successive R–R interval and high-frequency spectral power divided by heart rate increased until 2015, which were stable over 2016–2018 but decreased in 2019. The number of top-3 positions was related to the total (r = .66, P = .02) and LIT (r = .92, P < .001) volume and to several markers of supine parasympathetic activity. Conclusion: The improvement in performance of the participant was mainly determined by the progressive increase in training volume, especially performed at low intensity, and was correlated to parasympathetic activity markers. This case study confirms the effectiveness of the training method, with a large amount of LIT in an elite endurance athlete, and of regular HRV monitoring.
The Development of a Behavior Checklist for Mentally Tough Behaviors in Volleyball
Authors: Madrigal Leilani 
Published: September 2020

Mental toughness is a factor related to performance, better coping, and increased confidence. There has been a growing trend toward assessing mental toughness behaviorally. The purpose of this paper was to develop a behavioral assessment of mental toughness in volleyball. Following a five-stage process to develop a systematic observation instrument, the current study identified 10 mental toughness behaviors in volleyball, specifically, six behaviors occurring during a play and four behaviors after a play (i.e., when a point is scored from the opposing team). Furthermore, eight behaviors represent mentally tough actions, while two behaviors represent mentally weak actions. The results indicate that the behavioral checklist is a reliable systematic observation instrument. Coaches and certified mental performance consultants can benefit from using this checklist by discussing mental toughness and behaviors corresponding to mental toughness during game play, and then have a quantifiable way to track behaviors with individuals and volleyball teams.
Low mortality oxidative stress murine chronic wound model
Authors: Panayi Adriana C , Endo Yori , Karvar Mehran , Sensharma Prerana , Haug Valentin , Fu Siqi , Mi Bobin , An Yang , Orgill Dennis P 
Published: September 2020

IntroductionInvestigators have struggled to produce a reliable chronic wound model. Recent progress with antioxidant enzyme inhibitors shows promise, but mortality rates are high. We modified the dosage and administration of an antioxidant enzyme inhibitor regimen to reduce mortality while inducing a chronic wound environment.Research design and methodsTo chemically induce a chronic wound environment, we applied modified doses of catalase (3-amino-1,2,4-triazole; intraperitoneal 0.5 g/kg) and glutathione peroxidase (mercaptosuccinic acid; topical 300 mg/kg) inhibitors to the dorsal wounds of 11-week-old db/db mice. A cohort of these mice was treated with a collagen-glycosaminoglycan scaffold. Both groups were compared with Diabetic control mice.ResultsThis study successfully induced a chronic wound in 11-week-old db/db mice, with no animal deaths. The antioxidant enzyme treated groups showed delayed wound contraction and significantly higher levels of inflammatory tissue, collagen deposition, cellular proliferation and leukocyte infiltration than the Diabetic control group. Angiogenesis was significantly higher in the antioxidant enzyme treated groups, but the vessels were immature and friable. Scaffold engraftment was poor but appeared to promote blood vessel maturation.ConclusionsOverall, the two in vivo groups treated with the antioxidant enzyme inhibitors appeared to be arrested in the inflammatory stage of wound healing, while the Diabetic control group progressed to the maturation phase and ultimately remodeling. This model may be instrumental for the development of new wound therapeutics.
Effect of alcohol consumption and the presence of fatty liver on the risk for incident type 2 diabetes: a population-based longitudinal study
Authors: Okamura Takuro , Hashimoto Yoshitaka , Hamaguchi Masahide , Obora Akihiro , Kojima Takao , Fukui Michiaki 
Published: September 2020

IntroductionBoth fatty liver disease (FLD) and alcohol consumption have been reported to affect incident type 2 diabetes mellitus. The aim of this study was to evaluate the combined effect of FLD and alcohol consumption on incident type 2 diabetes.Research design and methodsIn this historical cohort study involving 9948 men, we investigated the influence of the presence of FLD and the grades of alcohol consumption on incident type 2 diabetes using Cox proportional hazards models. We categorized the participants into the following four groups: none or minimal alcohol consumption, <40 g/week; light, 40–140 g/week; moderate, 140–280 g/week; or heavy alcohol consumption, >280 g/week. FLD was diagnosed by abdominal ultrasonography.ResultsDuring the median 6.0-year follow-up, 568 participants developed type 2 diabetes. Heavy alcohol consumers with FLD showed a higher risk for developing type 2 diabetes compared with the other groups. Moderate alcohol consumers without FLD had a significantly higher risk for developing incident type 2 diabetes, compared with none or minimal and light alcohol consumers without FLD. In contrast, there was no apparent difference in the risk for incident type 2 diabetes between none or minimal, light, and moderate alcohol consumers with FLD. Furthermore, there was no statistically significant difference in the risk for incident type 2 diabetes between a moderate and heavy alcohol consumer without FLD and a none or minimal, light, and moderate alcohol consumer with FLD.ConclusionsTo prevent incident type 2 diabetes, we should acknowledge that the impact of alcohol consumption may vary in the presence of FLD.
Cell-based artificial APC resistant to lentiviral transduction for efficient generation of CAR-T cells from various cell sources
Authors: Schmidts Andrea , Marsh Leah C , Srivastava Ambike A , Bouffard Amanda A , Boroughs Angela C , Scarfò Irene , Larson Rebecca C , Bedoya Felipe , Choi Bryan D , Frigault Matthew J , Bailey Stefanie R , Leick Mark B , Vatsa Sonika , Kann Michael C , Prew Michelle S , Kleinstiver Benjamin P , Joung J Keith , Maus Marcela V 
Published: September 2020

BackgroundAdoptive cell therapy with chimeric antigen receptor T cells (CAR-T) has become a standard treatment for patients with certain aggressive B cell malignancies and holds promise to improve the care of patients suffering from numerous other cancers in the future. However, the high manufacturing cost of CAR-T cell therapies poses a major barrier to their broader clinical application. Among the key cost drivers of CAR-T production are single-use reagents for T cell activation and clinical-grade viral vector. The presence of variable amounts of contaminating monocytes in the starting material poses an additional challenge to CAR-T manufacturing, since they can impede T cell stimulation and transduction, resulting in manufacturing failure.MethodsWe created K562-based artificial antigen-presenting cells (aAPC) with genetically encoded T cell stimulation and costimulation that represent an inexhaustible source for T cell activation. We additionally disrupted endogenous expression of the low-density lipoprotein receptor (LDLR) on these aAPC (aAPC-ΔLDLR) using CRISPR-Cas9 gene editing nucleases to prevent inadvertent lentiviral transduction and avoid the sink effect on viral vector during transduction. Using various T cell sources, we produced CD19-directed CAR-T cells via aAPC-ΔLDLR-based activation and tested their in vitro and in vivo antitumor potency against B cell malignancies.ResultsWe found that lack of LDLR expression on our aAPC-ΔLDLR conferred resistance to lentiviral transduction during CAR-T production. Using aAPC-ΔLDLR, we achieved efficient expansion of CAR-T cells even from unpurified starting material like peripheral blood mononuclear cells or unmanipulated leukapheresis product, containing substantial proportions of monocytes. CD19-directed CAR-T cells that we produced via aAPC-ΔLDLR-based expansion demonstrated potent antitumor responses in preclinical models of acute lymphoblastic leukemia and B-cell lymphoma.ConclusionsOur aAPC-ΔLDLR represent an attractive approach for manufacturing of lentivirally transduced T cells that may be simpler and more cost efficient than currently available methods.