bluuebunny/crossref_metadata_2025_split huggingface dataset:
Epigenetic alterations of the promoter region of the POMC gene in adolescent depressive disorder patients with non-suicidal self-injury behaviors
Authors: Zheng Doudou , Bi Xiaojiao , Zhang Tianliang , Han Chao , Ma Tantan , Wang Lina , Sun Mengmeng , Cui Kaiyan , Yang Limin , Liu Lanfen 
Published: July 2020

Abstract
Background

The incidence of non-suicidal self-injury (NSSI) behavior among adolescents increases year by year. Patients with a history of both depression and NSSI behaviors tend to have greater risk of suicide. At present, the mechanism of adolescent depressive disorder patients with NSSI behaviors is not clear, epigenetic mechanism may be involved. Proopiomelanocortin (POMC) gene may be associated with depressive disorder. The purpose of this study was to investigate deoxyribonucleic acid (DNA) methylation of POMC gene promoter region of adolescent depressive disorder patients with NSSI behaviors.
Methods

Bisulfite Sequencing PCR (BSP) was used to test the methylation level of POMC promoter of 15 adolescent depressive disorder patients with NSSI behaviors and 15 healthy controls (HC). The online software Methylation Share Rate (MSR) Calcalate was used to draw the lollipop model of the methylation state. Self-made questionnaires were used to collect clinical data of the case group and control group. Hamilton depression scale-24 (HAMD-24), Hamilton anxiety scale (HAMA), Symptom Checklist-90 (SCL-90) were used to evaluate the characteristics and severity of depressive, anxiety and psychotic symptoms. Adolescent self-injury questionnaire was used to assess NSSI behaviors and its severity.
Results

BSP analysis found that the POMC methylation level of cytosine-guanine dinucleotide 1 (CpG1) site was higher in adolescent depressive disorder patients with NSSI behaviors than that of HC (P < 0.05). Overall significance in POMC methylation at CpG1 sites between adolescent depressive disorder patients with NSSI behaviors and HC was gender-independent, methylation level at CpG1 sites was higher in both male and female patients than that in HC. The CpG1 methylation had correlation with the family history (P < 0.05). We also found that POMC methylation level at CpG17 sites in female patients was significantly higher than that of the female HC (P < 0.05).
Conclusions

There was abnormal methylation in the POMC promoter region of adolescent depressive disorder patients with NSSI behaviors, the methylation of CpG1 sites may act as epigenetic markers for those adolescents.
Purification, Characterization, and Self-Assembly of the Polysaccharides from Allium Schoenoprasum
Authors: Zhang Fengrui , Zheng Jun , Li Zeyu , Cai Zixuan , Wang Fengqiao , Feng Liubin , Yang Dong 
Published: July 2020

Abstract

Background: Allium schoenoprasum is a world-wide common vegetable while only its leave is used in the food factory. Its stalk is largely discarded, for potential heavy metal accumulations, which eventually lead to an environmental contamination. To fully utilize this vegetable and minimize its metal content, the major polysaccharide content is purified and characterized with chemical and computational approaches.Results: The major polysaccharide component from the stalk of Allium schoenoprasum (AssP) was extracted and purified. The gel filtration chromatography purified AssP exhibited a molecular weight of around 1.6 kDa, which was verified by MALDI-ToF-MS. Monosaccharide analysis revealed its composition as rhamnose: arabinose: galactose: glucose: mannose: fructose with a molar ratio of 0.0264:2.46:3.71:3.35:1.00:9.93, respectively. Multiple NMR analysis revealed its backbone as α-Ara/Glu/Gal-(1→2)-linked and β-D-Fru-(4→5)-linked sugar residues. There was no tertiary structure of this polysaccharide, however, it self-assembled into a homogenous nanoparticle with a diameter of ~600 nm. The solution behavior of this AssP polysaccharide was simulated, and there was no specific binding site on one molecule for another. Association of this polysaccharide was concentration dependent. As the AssP concentration increased, the spherical particles increased their sizes and eventually merged into cylindrical micelles. The diversity of AssP hydrodynamic behavior endowed potential versatility in its applications.Conclusions: AssP was characterized as a polysaccharide with identified monosaccharide compositions and linkage between them. Although there is no tertiary structure in one AssP molecule, self-assembly of AssP molecules could form nanoparticles or micelles depending on its solution concentrations. The unique AssP solution behavior endows itself a potential biomaterial for nanoparticles preparations.
A population-based cohort study on the association of dietary patterns with sleep duration: A Joint modeling by mental health status
Authors: Hosseini Somaye , Rikhtehgaran Reyhaneh Rikhtehgaran , Mohammadifard Noushin , Roohafza Hamidreza , Sadeghi Masoumeh , Mansourian Marjan , Sarrafzadegan Nizal 
Published: July 2020

Abstract
Background: We investigated the association of dietary patterns and sleep duration by controlling mental health status (MHS) using a joint modeling approach.Methods and materials: The population based cohort study was conducted within the framework of the Isfahan Cohort Study (ICS), including 1383 participants, aged ≥ 35 years were followed from 2007 to 2013. A validated food frequency questionnaire was applied to obtain dietary patterns. Anthropometric measurements, blood pressure, fasting serum lipids and blood sugars were evaluated in both phases of the study. A random effect joint negative binomial and ordinal model were used to estimate diets effect on sleep duration and MHS.Results: Three dietary patterns were identified: Healthy, Iranian and Western dietary patterns. After adjustment for potential confounders, the higher scores of Western diet were associated with sleep hours (OR=1.08 and 95% CI: 1.02‒1.12), the higher scores of the Western diet during the 5 year follow-up was associated with greater sleep duration. Sleep duration and MHS were adversely associated (-0.097 and 95% CI: -0.09‒-0.05)Conclusion: Joint modeling by MHS, sleep duration was positively affected only by western diet. Iranian and healthy diet weren’t associated by sleep hours.
Anti- SARS-CoV-2 main protease complex (Mpro) activity of Palmatine
Authors: JADHAV VYANKATESH 
Published: July 2020

Abstract
The Pandemic situation caused due to SARS-CoV-2 causing Coronavirus Disease (CoVID-19) around globe. Recent, COVID-19 main protease complex (Mpro), highly modulating enzyme in SARS-CoV-2 was reported for viral replication and transcription. This multifunctionality of Mpro attracts for identification of potential drug target. Considering impact, In silico analysis was performed for Palmatine alkaloid against Mpro. Naturally, present in Tinospora cordifolia, found effective against Cancer, HIV, viral infections, diabetics. In methods, physico-chemical analysis by ProtParam tool and Structure of Mpro was predicted by SWISS-MODEL Workspace homology modeling server. Superimposition Structure and significant equal QMQE, QSQE values were found for eight highly similar templates. Structural assessment validation by Ramachandran plot (97.67% favoured), Local Quality estimate ratio (>0.6) and higher QMEAN score (y-axis). Further, docking was performed with validated Mpro model by SwissDock server. Interaction with -8.281919 ΔG indicates reliable Interaction. Also, comparative docking reveals, most favoured Palmatine interaction. Thus, an attempt was made to find potent inhibitor for SARS-CoV-2, as there is no promising and specific anti-viral drug or vaccine available for prevention and treatment of infections. However, In Vitro studies are required. Toxicity studies reported against Palmatine for acute effect (135 mg/kg body weight) on mouse model LD 50.
Towards an Ontology-Based Decision Support System for Syndrome-Differentiation and Treatment of Psoriasis Vulgaris in Traditional Chinese Medicine
Authors: Long Hai , Wang Zhe , Cui Yidi , Wang Junhui , Gao Bo , Chen Chao , Herre Heinrich , Zhu Yan 
Published: July 2020

Abstract
Background: Psoriasis is a chronic, non-communicable, painful, disfiguring and disabling disease, which is not curable and strongly declines the patients’ quality of life (QoL). However, diagnose and treatment in traditional Chinese medicine (TCM) based on syndrome differentiation has been used in practice for a long time and has also achieved some effect. Though, up to now, only few studies are available reporting on the use of semantic technologies and pertain to knowledge systems that use TCM-syndrome differentiation for information retrieval and automated reasoning. Nowadays, the diagnosis in TCM relies mainly on the personal expertise and clinical experience of the doctors. For various reasons, misdiagnoses or missed diagnoses cannot be completely excluded, leading to unexpected results.Methods: Firstly, we developed a domain ontology for syndrome differentiation of psoriasis vulgaris. For this purpose, we used the ontology editor Protégé and applied a top-down approach which adopts the framework of general formal ontology (GFO) and its middle-level core ontology GFO-TCM. Furthermore, we implement a prototype which is based on this ontology. Additionally, we also used a case-database for CBR (Case Based Reasoning) combined with fuzzy pattern recognition.Results: A prototype for diagnosis and treatment of psoriasis vulgaris, named ONTOPV-system, is proposed which is based on the principle of syndrome differentiation and treatment in TCM; this system realizes an expert-assisted decision support method which relies on a domain ontology, uses fuzzy logic reasoning, and case retrieval and is intended to support clinical diagnostic decisions for TCM practitioners.Conclusions: We designed and implemented a prototype for psoriasis diagnosis in terms of syndrome differentiation. The system can not only realize the basic functionalities of data collection, querying, browsing and navigation, but also support rule-based knowledge reasoning and customize approximate reasoning based on CBR through fuzzy logic, which can provide users with clinical decision support for TCM syndrome differentiation in diagnosis of psoriasis. In addition, it comprises a domain knowledge base of psoriasis, which is developed based on the GFO framework with good extensibility.
The Prevalence of Human Papillomavirus Among the Indigenous Population&nbsp;in Serian, Sarawak, Malaysian Borneo
Authors: Tan Cheng Siang , Yi Myat San , Lwin Soe , Jerip Abigail Rembui , Kipli Mardiana 
Published: July 2020

Abstract
Introduction: Cervical cancer remains a significant problem worldwide, particularly in resource-limited countries, and having persistent HRHPV infection is a necessary risk factor. HPV16/18 are generally regarded as the cause of 70% cervical cancer incidences worldwide, and effective vaccines have been developed against these two HPVs. Although Malaysia has officially adopted an HPV vaccination strategy into the National Vaccination Program, the comprehensive prevalence data on each endemic HPV genotypes was absent in East Malaysia and most parts of the country except for major cities in Peninsular Malaysia.Objective: The objective of this study is to elucidate the endemic HPV genotypes that are circulating in the suburban population in Serian Division, Sarawak, Malaysia.Methods:Non-virgin women who were attending the Women's health campaign on the 5th and 23rd October 2018 were recruited. Informed consent was obtained, and a cervical swab was clinician-collected. The presence of HPV in the cervical swab was detected using MY09/MY11 followed by GP5+/GP6+ nested-PCR and its corresponding genotypes identified via sequencing. HRHPV positive women were screened using Results: We have recruited 43 sexually active women with median age of 51-year-old. HPV18, 39, 52, 56, and 84 were detected at an equal ratio. The overall prevalence rate of HPV, HRHPV and URHPV were 11.62% (5/43), 9.3% (4/43) and 2.3% respectively. Only 20% (n=1/5) of the HRHPV positive women were positive by VIA.Conclusion: HPV18, 39, 52, 56, and 84 were detected in Serian at an equal ratio. HPV16 was absent, and HPV18 was the only vaccine-genotype detected. HPV 39, 52, 56, and 84 were not covered by the bivalent and tetravalent HPV vaccines.
Early Esophageal Squamous Cell Carcinoma and Precancerous Lesions can be Detected by White Light Endoscopy with the Aid of White Substance
Authors: Yang Guodong , Wang Yingbang , Zhang Xiaoying , Zhang Xiaoming , Wu Linyi , Yu Xiaogang , Zhao Mu , Chen Yuling , Li Zhen 
Published: July 2020

Abstract
Objective: To investigate the diagnostic value of white substance in the detection of flat esophageal mucosal lesions and precancerous lesions associated with early esophageal squamous cell carcinoma Methods: Clinical and pathological data of patients diagnosed with flat esophageal mucosal disease were collected. The lesions were divided into a neoplastic group and a non-neoplastic group, and the clinicopathological differences between the groups were analyzed. The patients were also divided into two groups based on the presence of white substance after endoscopic examination: a “white substance” group and a “non-white substance” group. The differences between the two groups were analyzed. The diagnostic value of white substance for detection of early esophageal squamous cell carcinoma and related precancerous lesions was calculated and the pathological nature of white substance inferred. Results: In total, 183 patients with flat esophageal mucosal lesions were enrolled, including 92 (50.3%) with neoplastic lesions and 91 (49.7%) with non-neoplastic lesions. Forty-nine cases (26.8%) presented white substance in the esophageal mucosa. White substance was mainly found in female patients (57.1%), middle esophagus (75.5%), and 60-69-year-old patients (51.0%). Moreover, white substance was more frequently found in neoplastic than in non-neoplastic lesions (43.5% vs. 11.0%, p < 0.05). Consistently, neoplastic lesions were more represented in the white substance group than in the non-white substance group and the difference was statistically significant (79.6 vs. 20.4%, p < 0.05). 51% of the patients in the white substance group, but only 26.1% of those in the non-white substance group, had hyperkeratosis and necrosis, and the difference was statistically significant (p < 0.05). The diagnostic sensitivity of white substance for detection of early esophageal squamous cell carcinoma and precancerous lesions was 42.4% (95% CI, 32.8%–52.6%),the specificity 89.0% (95% CI, 80.8%–94.1%).Conclusions: White substance in the mucosa of flat esophageal lesions exhibited high specificity for the diagnosis of early esophageal squamous cell carcinoma and precancerous lesions. Thus, the presence of white substance in the esophageal mucosa, even in the absence of obvious lesions, may reflect the presence of latent early esophageal squamous cell carcinoma and precancerous lesions. White substance was associated with hyperkeratosis and necrosis.
Treatment of Aneurysmal Bone Cysts with Titanium Elastic Nails in Children
Authors: Wang Yi-chen , Jia Xing , Shen Yang , Wang Sun , Dong Liang-chao , Ren Jing , Zhao Li-hua 
Published: July 2020

Abstract
Background: The main treatment method of the primary aneurysmal bone cyst (ABC) is to curettage and bone grafts with high-speed burring, radiotherapy, sclerotherapy, arterial embolism and hormone therapy can be used for the lesions whose location cannot be easily exposed by the surgery. Regardless of the method, high recurrence rates are a common problem. The purpose of this study was to evaluate retrospectively the use of titanium elastic nails as a internal fixation in the treatment of aneurysmal bone cysts in children.Methods: Children with histological primary aneurysmal bone cyst were evaluated between 2010 to 2017. The patients were divided into 2 groups according to the treatment plan. Patients in the study group operated with curettage and bone grafts with high-speed burring + internal fixation of titanium elastic nails (TEN), and patients in the control group operated with curettage and bone grafts with high-speed burring. The curative effect of the children in the 2 groups were analyzed statistically according to the imaging results (Neer grading) and MSTS functional evaluation.Results: A total of 26 ABC patients (mean 6.5 years at surgery; 21 males;5 female) met the inclusion criteria and were evaluated between 2010 to 2017 after a mean follow-up of 5.2 years (range, 2.5-8.4 years), 16 of which in the study group and 10 in the control group. 9 of the 26 patients recurred (3 cases study group；6 in the control group；). According to the imaging results (Neer grading) and MSTS function evaluation, there was statistically significant difference in the curative effect between the 2 groups (P＜0.05). The recurrence cases in the study group had a better MSTS functioncal recovery.Conclusion: The use of TEN can significantly improve the cure rate, reduce the recurrence rate and improve the quality of life for children with primary aneurysmal bone cyst.
Antibiotic resistance patterns of efflux pumps MexAB-Opr M in pathogenic Pseudomonas aeruginosa isolates
Authors: rasouli Javad , hashemi Behnam , Afkhami Hamed , Khaledi Mansoor , valadan Reza , Fathi Javad , ahanjan mohammad 
Published: July 2020

Abstract
Objectives

Pseudomonas aeruginosa is one of the most important causes of Hospital infection especially in burn victims. The current study aimed to determine antibiotic resistance of the efflux Pumps MexAB-Opr M. In the present study, 115 samples of urine, blood, sputum, and ICU were collected from the reconstructive section of the patients. The drug susceptibility patterns were determined by disk diffusion method. Phenotypic activity of the efflux pump from the E-test was evaluated, in the presence and without the presence of efflux pump inhibitor. The MexAB gene was analyzed by PCR reaction.
Results

The resistant isolated was shown to be Ciprofloxacin 33.91%, Nurfloxacin 38.26%, Gentamicin 71.7%, Nalidixic acid 95.95%, Ceftazidim 38.46%, Emipenem 24.34%, Meropenem 26.36%, and Cefotaxim 40.86%. The highest and lowest resistance rates were Co-trimoxazole and Piperacilin, respectively. The findings of PCR reaction among 115 P. aeruginosa isolates indicated that 62.62% was MexAB gene. The results of MIC with E-test revealed that the role of efflux pumps in antibiotic resistance was 19 isolated. Due to the importance of antibiotic resistance to investigate other efflux pumps, comparison of efflux pump involvement in antibiotic resistance, and relationship between efflux pumps MexAB-Opr M are highly required and suggested.
Design and Analysis of a Novel Compliant Mechanism with RPR Degrees of Freedom
Authors: Zhang Shuang , Liu Jing-Fang , Ding Hua-Feng 
Published: July 2020

Abstract
Magnetic drive pump has gotten great achievement A novel compliant mechanism with RPR degrees of freedom (DOF) is proposed where R and P represent rotation and translation DOFs, respectively. The proposed compliant mechanism is obtained from dimension synthesizing a 2-RPU-UPR rigid parallel mechanism with the method of optimization of motion/force transfer characteristic, where R, P and U represent rotation, translation and universal pairs, respectively. Firstly, inverse kinematics and Jacobian matrix are analyzed for the dimensional synthesis. Then, output transmission indexes of branches in the parallel mechanism are given. Dimensional synthesis is completed based on the normalized design parameter. And optimization of flexure joints based on constrained energy is carried out. Afterwards, the novel compliant mechanism is obtained by direct replacing method. Mechanical model of the compliant mechanism including static stiffness and input stiffness is built based on the pseudo-rigid body model method and virtual work principle for its future application. Finally, FEA simulation by Ansys Workbench is carried out to verify DOF, effectiveness of the dimension synthesis, and compliant model. Optimization of motion/force transfer characteristic is first applied for the design of compliant mechanisms to suppress drift of rotation axis in the paper.
Exploration of the mechanism of Ziyin Tongluo Formula on preventing and treating postmenopausal osteoporosis based on the network pharmacology
Authors: Chen Rong-Bin , Yang Ying-Dong , Sun Kai , Liu Shan , Guo Wei , Zhang Jin-Xin , Li Yong 
Published: July 2020

Abstract
Background

Postmenopausal osteoporosis (PMOP) is a global chronic and metabolic bone disease that poses huge challenges to individuals and society. Previous studies have confirmed that Ziyin Tongluo Formula (ZYTLF) has a good clinical effect in the treatment of PMOP. However, the material basis and mechanism of ZYLTF against PMOP has not been thoroughly explained.
Methods

TCMSP, TCMID, and BATMAN-TCM databases were used to identify the active ingredients and their putative targets. Genes associated with PMOP were mined from GeneCards, OMIM, DisGeNET databases, and then mapped with the putative targets to obtain overlapping target genes. A network model of "herb-active ingredient-overlapping target genes" was constructed and a protein-protein interaction network of overlapping target genes was built and the key genes were selected based on the MCC algorithm. The key genes were imported to the DAVID database to performs GO and KEGG pathway enrichment analyses.
Results

Ninety-two active components of ZYTLF corresponded to 243 targets, with 129 target genes interacting with PMOP, and 50 key genes were selected. GO analysis results showed that biological process mainly included positive regulation of transcription, negative regulation of apoptosis, and cell components were mainly nucleus, cytoplasm, and molecular functions mainly included enzyme binding, protein binding and transcription factor binding. There were two main types of significant KEGG pathways in PMOP, hormone-related signaling pathways (estrogen, prolactin, thyroid hormone) and inflammation-related pathways (TNF, PI3K-Akt, MAPK ).
Conclusions

The underlying therapeutic mechanisms of ZYTLF action on PMOP maybe is that, the active ingredients such as quercetin, kaempferol, luteolin act on ESR1, TNF, IL6, MAPK8 and other key genes, which mainly enrich in estrogen, TNF, PI3K-Akt, MAPK and other signaling pathways.
NRCMS and Rural Household Credit Availability in China: Evidence From CFPS
Authors: Liu Jin , Xu Qing , Yang Qing , Lu Yufeng 
Published: July 2020

Abstract
Background: The Chinese government has, in seeking to enable farmers to obtain and use safe and affordable formal financial services, implemented Inclusive Finance. But disease burden, poor health and other factors have made it difficult for farmers to obtain formal credit servicesMethods: This paper draws on 2010 China family survey (CFPS) data to explore how the New Rural Cooperative Medical Scheme (NRCMS) affected rural households’ credit availability. Results:The results show that, as a public health insurance system sustained by the participation of government investment participation, the NRCMS provides good ‘collateral’ and significantly enhances farmers’ credit availability level. Conclusions: Our study suggests the positive impacts is mainly reflected in the economic effect of the NRCMS. This raises the question of how to make better use of the NRCMS’ mortgage function, promote inclusive finance and provide more financial services for farmers that will promote their income. This serves to confirm and underline the importance of relevant policies.JEL Classifications: I18; G50; O16