bluuebunny/crossref_metadata_2025_split huggingface dataset:
Justice sensitivity and rejection sensitivity as predictors and outcomes of eating disorder pathology: A 5‐year longitudinal study
Authors: Bondü Rebecca , Bilgin Ayten , Warschburger Petra 
Published: April 2020

AbstractObjectiveRejection sensitivity and justice sensitivity are personality traits that are characterized by frequent perceptions and intense adverse responses to negative social cues. Whereas there is good evidence for associations between rejection sensitivity, justice sensitivity, and internalizing problems, no longitudinal studies have investigated their association with eating disorder (ED) pathology so far. Thus, the present study examined longitudinal relations between rejection sensitivity, justice sensitivity, and ED pathology.MethodParticipants (N = 769) reported on their rejection sensitivity, justice sensitivity, and ED pathology at 9–19 (T1), 11–21 (T2), and 14–22 years of age (T3).ResultsLatent cross‐lagged models showed longitudinal associations between ED pathology and anxious rejection sensitivity, observer and victim justice sensitivity. T1 and T2 ED pathology predicted higher T2 and T3 anxious rejection sensitivity, respectively. In turn, T2 anxious rejection sensitivity predicted more T3 ED pathology. T1 observer justice sensitivity predicted more T2 ED pathology, which predicted higher T3 observer justice sensitivity. Furthermore, T1 ED pathology predicted higher T2 victim justice sensitivity.DiscussionRejection sensitivity—particularly anxious rejection sensitivity—and justice sensitivity may be involved in the maintenance or worsening of ED pathology and should be considered by future research and in prevention and treatment of ED pathology. Also, mental health problems may increase rejection sensitivity and justice sensitivity traits in the long term.
Harnessing knowledge of animal behavior to improve habitat restoration outcomes
Authors: Hale Robin , Blumstein Daniel T. , Mac Nally Ralph , Swearer Stephen E. 
Published: April 2020

AbstractRestoring degraded habitat to increase biodiversity is a global challenge. While habitat restoration for animals should lead to self‐sustaining breeding populations of target species, often this does not occur. Understanding the factors constraining progress toward this goal and how these constraints can be overcome is vital. We use a review to highlight how insights from animal behavior can help plan restoration projects, and identify and ameliorate some of the reasons why restoration may fail to meet biodiversity goals. We present a decision tree to highlight how behavioral knowledge can identify and address two ways in which restoration can fail when: (1) target animals do not colonize restored sites and (2) they colonize restored sites but experience conditions that do not match their habitat requirements. Investing in the collection of behavioral information may be difficult for management agencies when funding is limited. We highlight when behavioral information is likely to be most important, and some of the practical considerations for its application in restoration projects. We conclude by identifying key knowledge gaps and future directions that can improve restoration outcomes for biodiversity by incorporating behavioral knowledge. Restoration is needed to ameliorate the effects of habitat loss, degradation, and fragmentation on fauna. Unfortunately, restoration does not always lead to the intended optimal outcomes for target animals. A greater consideration of animal behavior and its consequences can help during both the planning and evaluation of restoration projects. We hope to stimulate dialogue between restoration and behavioral ecologists to improve restoration outcomes for animals.
Enhancement of Selective Fixation of Dinitrogen to Ammonia under Modal Strong Coupling Conditions
Authors: Oshikiri Tomoya , Shi Xu , Misawa Hiroaki 
Published: April 2020

AbstractInvited for the cover of this issue is the group of Hiroaki Misawa from Hokkaido University, Sapporo, Japan. The cover image shows a two‐electrode system for ammonia photosynthesis composed of a strong coupling photoanode and a Zr mesh cathode.
Unsettling ourselves: Notes on reflective listening beyond discomfort
Authors: Petillo April 
Published: April 2020

AbstractReflecting on Melissa Harris‐Perry's 2016 American Anthropological Association (AAA) keynote address and conversations that followed, this semi‐autoethnographic exploration considers disciplinary tensions and complicities concerning our (un)willingness to hear content when discomforted by academic affect. The article considers why feminist anthropologists might “listen through discomfort,” a process Petillo describes as “embodied listening where self‐reflection is deeply entangled,” and advocates including this in intersectional feminist, queer activist ethnographic praxis. Attuned to the benefits of feminist activist ethnographic self‐corrective action (Morgensen 2013) and aware that research is always somewhat performative (Oikarinen‐Jabai 2003), Petillo focuses on the enhanced potential of embodied listening—a listening reliant on kinesthetic literacy (Gill 2012, 33) turned inward or the ability to read our own “vocabulary of actions.” Through notes and intimately personal observations, Petillo highlights where self‐reflective embodied listening through the “discomforts” of engaging lived realities without “academic affect” affords the feminist, queer ethnographic activist a path for transdisciplinary listening across identities and intimacies.
Human Leukocyte Antigen B*14:01 and B*35:01 Are Associated With Trimethoprim‐Sulfamethoxazole Induced Liver Injury
Authors: Li Yi‐Ju , Phillips Elizabeth J. , Dellinger Andrew , Nicoletti Paola , Schutte Ryan , Li Danmeng , Ostrov David A. , Fontana Robert J. , Watkins Paul B. , Stolz Andrew , Daly Ann K , Aithal Guruprasad P , Barnhart Huiman , Chalasani Naga ,   the Drug‐induced Liver Injury Network
Published: April 2020

Background and Aims
Trimethoprim (TMP)–sulfamethoxazole (SMX) is an important cause of idiosyncratic drug‐induced liver injury (DILI), but its genetic risk factors are not well understood. This study investigated the relationship between variants in the human leukocyte antigen (HLA) class 1 and 2 genes and well‐characterized cases of TMP‐SMX DILI.

Approach and Results
European American and African American persons with TMP‐SMX DILI were compared with respective population controls. HLA sequencing was performed by Illumina MiSeq (Illumina, San Diego, CA) for cases. The HLA genotype imputation with attribute bagging program was used to impute HLA alleles for controls. The allele frequency difference between case patients and controls was tested by Fisher’s exact tests for each ethnic group. For European Americans, multivariable logistic regression with Firth penalization was used to test the HLA allelic effect after adjusting for age and the top two principal components. Molecular docking was performed to assess HLA binding with TMP and SMX. The European American subset had 51 case patients and 12,156 controls, whereas the African American subset had 10 case patients and 5,439 controls. Four HLA alleles were significantly associated in the European American subset, with HLA‐B*14:01 ranking at the top (odds ratio, 9.20; 95% confidence interval, 3.16, 22.35; P = 0.0003) after covariate adjustment. All carriers of HLA‐B*14:01 with TMP‐SMX DILI possessed HLA‐C*08:02, another significant allele (P = 0.0026). This pattern was supported by HLA‐B*14:01–HLA‐C*08:02 haplotype association (P = 1.33 × 10−5). For the African American patients, HLA‐B*35:01 had 2.8‐fold higher frequency in case patients than in controls, with 5 of 10 patients carrying this allele. Molecular docking showed cysteine at position 67 in HLA‐B*14:01 and phenylalanine at position 67 in HLA‐B*35:01 to be the predictive binding sites for SMX metabolites.

Conclusions

HLA‐B*14:01 is associated with TMP‐SMX DILI in European Americans, and HLA‐B*35:01 may be a potential genetic risk factor for African Americans.
B‐cell maturation antigen‐specific chimeric antigen receptor T cells for multiple myeloma: Clinical experience and future perspectives
Authors: Sellner Leopold , Fan Fuli , Giesen Nicola , Schubert Maria‐Luisa , Goldschmidt Hartmut , Müller‐Tidow Carsten , Dreger Peter , Raab Marc S. , Schmitt Michael 
Published: April 2020

AbstractDespite major advances in the treatment of multiple myeloma (MM), it remains a largely incurable disease with long‐term control often dependent on continuous therapy. More effective, better tolerated treatments are therefore required to achieve durable remissions and to improve the quality of life of MM patients. Adoptive immunotherapy employing T cells expressing chimeric antigen receptors (CAR) is currently among the most promising treatment approaches in cancer. Within the target portfolio for MM immunotherapy, B‐cell maturation antigen (BCMA) is among the most widely studied target antigens. BCMA is consistently expressed on MM cells and, importantly, is not expressed in critical healthy tissue. For this reason, it is an ideal target for MM immunotherapy. Several clinical trials evaluating different BCMA‐targeting CAR constructs have been initiated and early results are very promising. However, in this rapidly developing clinical landscape, the ultimate role of BCMA‐specific CAR‐T cell therapy remains unclear. In this review, we will summarize currently available clinical data on BCMA‐directed CAR‐T cells and discuss potential future perspective for this promising treatment approach in MM.
C─H⋯O hydrogen bonds in kinase‐inhibitor interfaces
Authors: Derewenda Zygmunt S. , Hawro Izabela , Derewenda Urszula 
Published: April 2020

AbstractC─H⋯O hydrogen bonds constitute a unique class of cohesive interactions. Their properties are similar to those of canonical H‐bonds, although their energy is significantly lower, typically in the 0.5–2.5 kcal/mol range. Polarised C─H groups, such as those adjacent to electronegative groups, or within aromatic moieties, are particularly strong donors. C─H⋯O bonds are ubiquitous in nucleic acids and in proteins, notably stabilizing the β‐sheet secondary structure. They have also been observed in numerous protein‐ligand interactions. Here, we analysed crystal structures, deposited in the Protein Data Bank, of complexes of FDA‐approved protein kinase inhibitors with cognate kinases, to assess the possible role of C─Hinhibitor⋯Oprotein hydrogen bonds. The conserved hinge motif of protein kinases with two solvent‐exposed carbonyl groups and one exposed backbone amide, is well known to be involved in canonical H‐bonding with inhibitors. We now find that in virtually all complexes where the inhibitor interacts with the hinge backbone, at least one of the hinge carbonyl groups accepts an H‐bond from a C─H inhibitor group, which is either aromatic or adjacent to an electronegative group. These observations are important for design of hinge‐binding scaffolds of novel kinase inhibitors for therapeutic use.
The promise of regenerative medicine in the treatment of urogenital disorders
Authors: Keshel Saeed Heidari , Rahimi Azam , Hancox Zoe , Ebrahimi Maryam , Khojasteh Arash , Sefat Farshid 
Published: April 2020

AbstractPolymers and scaffolds are the most significant tools in regenerative medicine. Urogenital disorders are an important group of diseases that greatly affect the patient's life expectancy and quality. Reconstruction of urogenital defects is one of the current challenges in regenerative medicine. Regenerative medicine, as well as tissue engineering, may offer suitable approaches, while the tools needed are appropriate materials and cells. Autologous urothelial cells obtained from biopsy, bone marrow‐derived stem cells, adipose stem cells and urine‐derived stem cells that expressed mesenchymal cell markers are the cells that mainly used. In addition, two main types of biomaterials mainly exist; synthetic polymers and composite scaffolds that are biodegradable polymers with controllable properties and naturally derived biomaterials such as extracellular matrix components and acellular tissue matrices. In this review, we present and evaluate the most appropriate and suitable scaffolds (naturally derived and synthetic polymers) and cells applied in urogenital reconstruction.
Diagnostic Value of Magnetic Resonance Imaging in Fibrodysplasia Ossificans Progressiva
Authors: Botman Esmée , Teunissen Bernd P , Raijmakers Pieter , de Graaf Pim , Yaqub Maqsood , Treurniet Sanne , Schoenmaker Ton , Bravenboer Nathalie , Micha Dimitra , Pals Gerard , Bökenkamp Arend , Netelenbos J Coen , Lammertsma Adriaan A , Eekhoff Elisabeth MW 
Published: April 2020

ABSTRACTUsing [18F] Sodium Fuoride (NaF) Positron Emission Tomography (PET) it is not only possible to identify the ossifying potency of a flare‐up, but also to identify an asymptomatic chronic stage of fibrodysplasia ossificans progressiva (FOP). The purpose of this study was to investigate the diagnostic role of a more widely available imaging modality, Magnetic Resonance Imaging (MRI), which is of special interest for studies in pediatric FOP patients. MRI and [18F]NaF PET/CT images at time of inclusion and subsequent follow‐up CT scans of 4 patients were analyzed retrospectively. Presence, location, and intensity of edema identified by MRI were compared with activity on [18F]NaF PET. Occurrence or progression of heterotopic ossification (HO) was examined on the follow‐up CT images. Thirteen different lesions in various muscle groups were identified: five lesions with only edema, five lesions with both edema and increased [18F]NaF uptake, one lesion with only increased [18F]NaF uptake, and two lesions with neither edema nor uptake of [18F]NaF. Mild edema, found in three lesions, was present at asymptomatic sites, which did not show increased [18F] NaF uptake or progression of HO on consecutive CT images. Moderate edema was found in three symptomatic lesions, with increased [18F]NaF on PET and progression of HO on CT. Severe edema was identified in four lesions. Interestingly, two of these lesions did not develop HO during follow‐up; one of these two even gave obvious symptoms of a flare‐up. MRI can identify whether symptoms are the result of an acute flare‐up by the presence of moderate to severe edema. The occurrence of severe edema on MRI was not always related to an ossifying lesion. The additional diagnostic value of MRI requires further investigation, but MRI does not seem to fully replace the diagnostic characteristics of [18F]NaF PET/CT in FOP. © 2020 The Authors. JBMR Plus published by Wiley Periodicals, Inc. on behalf of American Society for Bone and Mineral Research.
College students’ attitudes, stigma, and intentions toward seeking online and face‐to‐face counseling
Authors: Bird Matthew D. , Chow Graig M. , Yang Yanyun 
Published: April 2020

AbstractObjective(s)To investigate differences in public stigma, self‐stigma, attitudes (value and discomfort), and intentions to seek help between online and face‐to‐face counseling. To identify a difference in the relationship between these variables and both counseling modalities.MethodAn online survey completed by 538 college students from one university in the Southeastern United States. The sample included 412 females and 126 males with a mean age of 20.21 years (standard deviation [SD] = 1.26).ResultsSignificantly higher levels of self‐stigma and discomfort toward online counseling were reported. Significantly higher value and intentions were reported toward face‐to‐face counseling. Self‐stigma was positively related to public stigma, value was negatively related to self‐stigma, and intentions toward seeking help was positively related to value.ConclusionsResults suggest face‐to‐face counseling is seen as a more favorable method of service delivery compared to online counseling. Value toward online counseling is an important predictor for seeking this type of help.
Pevonedistat, a NEDD8‐activating enzyme inhibitor, induces apoptosis and augments efficacy of chemotherapy and small molecule inhibitors in pre‐clinical models of diffuse large B‐cell lymphoma
Authors: Torka Pallawi , Mavis Cory , Kothari Shalin , Belliotti Sarah , Gu Juan , Sundaram Suchitra , Barth Matthew , Hernandez‐Ilizaliturri Francisco J. 
Published: April 2020

AbstractWe studied the biological activity of pevonedistat, a first‐in‐class NEDD8‐activating enzyme (NAE) inhibitor, in combination with various cytotoxic chemotherapy agents and small molecule inhibitors in lymphoma preclinical models. Pevonedistat induced cell death in activated B‐cell (ABC) diffuse large B‐cell lymphoma (DLBCL) cell lines and to a lesser degree in germinal center B‐cell (GCB) DLBCL cell lines. In pevonedistat sensitive cells, we observed inhibition of NF‐κB activity by p65 co‐localization studies, decreased expression of BCL‐2/Bcl‐XL, and upregulation of BAK levels. Pevonedistat enhanced the activity of cytarabine, cisplatin, doxorubicin, and etoposide in ABC‐, but not in the GCB‐DLBCL cell lines. It also exhibited synergy with ibrutinib, selinexor, venetoclax, and A‐1331852 (a novel BCL‐XL inhibitor). In vivo, the combination of pevonedistat and ibrutinib or pevonedistat and cytarabine prolonged survival in SCID mice xenograft models when compared with monotherapy controls. Our data suggest that targeting the neddylation pathway in DLBCL is a viable therapeutic strategy and support further clinical studies of pevonedistat as a single agent or in combination with chemotherapy or novel targeted agents.
Synthesis, anticancer, and computational studies of 1, 3, 4‐oxadiazole‐purine derivatives
Authors: Shahzadi Irum , Zahoor Ameer F. , Rasul Azhar , Rasool Nasir , Raza Zohaib , Faisal Shahla , Parveen Bushra , Kamal Shagufta , Zia‐ur‐Rehman Muhammad , Zahid Faisal M. 
Published: April 2020

AbstractTheophylline‐7‐acetic acid (acefylline) (3) and its derivatives are pharmacologically active compounds and generally recognized as bronchodilators for the treatment of respiratory diseases like acute asthma for over 70 years. In this article, synthesis of 2‐((5‐((1,3‐dimethyl‐2,6‐dioxo‐2,3‐dihydro‐1H‐purin‐7(6H)‐yl)methyl)‐1,3,4‐oxadiazol‐2‐yl)thio)‐N‐arylacetamides (10a‐j) has been reported. All the synthesized derivatives (10a‐j) were structurally verified by FT‐IR, 1H NMR, 13C NMR and evaluated for their anti‐cancer (using MTT assay), hemolytic and thrombolytic potential. N‐(4‐Chlorophenyl)‐2‐(5‐((1,3‐dimethyl‐2,6‐dioxo‐2,3‐dihydro‐1H‐purin‐7(6H)‐yl)methyl)‐1,3,4‐oxadiazol‐2‐ylthio)acetamide (10g) was found to be the most active against human liver cancer cell lines (Huh7) having cell viability 53.58 ± 1.28 using 100 μg/mL concentration of compound which was further in‐silico modelled to describe the possible mechanistic insights for its anti‐proliferative activity. The results of hemolytic and thrombolytic activities indicated that these derivatives were less toxic and hold considerable potential as a drug candidate. 2‐(5‐((1,3‐Dimethyl‐2,6‐dioxo‐2,3‐dihydro‐1H‐purin‐7(6H)‐yl)methyl)‐1,3,4‐oxadiazol‐2‐ylthio)‐N‐(2‐fluorophenyl)acetamide (10c) of the series was found to be least toxic with 0.1% hemolysis relative to ABTS (95.5%) as positive control. 2‐(5‐((1,3‐Dimethyl‐2,6‐dioxo‐2,3‐dihydro‐1H‐purin‐7(6H)‐yl)methyl)‐1,3,4‐oxadiazol‐2‐ylthio)‐N‐(tetrahydro‐2H‐pyran‐4‐yl)acetamide (10j) exhibited potent clot lysis activity (90%) as compared to negative control DMSO (0.57%).
Berberine suppresses influenza virus-triggered NLRP3 inflammasome activation in macrophages by inducing mitophagy and decreasing mitochondrial ROS
Authors: Liu Hui , You Leiming , Wu Jun , Zhao Mengfan , Guo Rui , Zhang Haili , Su Rina , Mao Qin , Deng Di , Hao Yu 
Published: April 2020

Abstract
Berberine (BBR) is an isoquinoline alkaloid extracted from several commonly used Chinese herbs. Our previous studies demonstrated BBR-mediated alleviation of lung injury due to inflammation and decrease in the mortality of mice with influenza viral pneumonia. The recent argument of autophagy against inflammatory responses has aroused wide concerns. This study focuses on the reactive oxygen species-Nod-like receptor protein 3 (ROS-NLRP3) pathway to investigate whether BBR inhibits NLRP3 inflammasome activation by inducing mitophagy. Our results demonstrate that BBR and mitochondrion-targeted superoxide dismutase mimetic (Mito-TEMPO; a specific mitochondrial ROS scavenger) significantly restricted NLRP3 inflammasome activation, increased mitochondrial membrane potential (MMP), and decreased mitochondrial ROS (mtROS) generation in J774A.1 macrophages infected with PR8 influenza virus. These observations suggest that the inhibitory effects of BBR on NLRP3 inflammasome activation were associated with the amelioration of mtROS generation. BBR treatment induced regular mitophagy, as evident from the increase in microtubule-associated protein 1 light chain 3 II, decrease in p62, colocalization of LC3 and mitochondria, and formation of autophagosomes. However, 3-methyladenine, an autophagy inhibitor, reversed the inhibitory effects of BBR on mitochondrial damage and NLRP3 inflammasome activation in influenza virus-infected macrophages, indicating the involvement of mitophagy in mediating the inhibitory effects of BBR on NLRP3 inflammasome activation. Furthermore, the knockdown of Bcl-2/adenovirus E18-19-kDa interacting protein 3 (BNIP3) expression attenuated the effects of BBR on mitophagy induction to some extent, suggesting that the BBR-induced mitophagy may be, at least in part, mediated in a BNIP3-dependent manner. Similar results were obtained in vivo using a mouse model of influenza viral pneumonia that was administered with BBR. Taken together, these findings suggest that restricting NLRP3 inflammasome activation by decreasing ROS generation through mitophagy induction may be crucial for the BBR-mediated alleviation of influenza virus-induced inflammatory lesions.
Unravelling the heterogeneity and dynamic relationships of tumor-infiltrating T cells by single-cell RNA sequencing analysis
Authors: Yu Xin , Zhang Lei , Chaudhry Ashutosh , Rapaport Aaron S. , Ouyang Wenjun 
Published: April 2020

Abstract
T cells are crucial for the success of immune-based cancer therapy. Reinvigorating antitumor T cell activity by blocking checkpoint inhibitory receptors has provided clinical benefits for many cancer patients. However, the efficacy of these treatments varies in cancer patients and the mechanisms underlying these diverse responses remain elusive. The density and status of tumor-infiltrating T cells have been shown to positively correlate with patient response to checkpoint blockades. Therefore, further understanding of the heterogeneity, clonal expansion, migration, and effector functions of tumor-infiltrating T cells will provide fundamental insights into antitumor immune responses. To this end, recent advances in single-cell RNA sequencing technology have enabled profound and extensive characterization of intratumoral immune cells and have improved our understanding of their dynamic relationships. Here, we summarize recent progress in single-cell RNA sequencing technology and current strategies to uncover heterogeneous tumor-infiltrating T cell subsets. In particular, we discuss how the coupling of deep transcriptome information with T cell receptor (TCR)-based lineage tracing has furthered our understanding of intratumoral T cell populations. We also discuss the functional implications of various T cell subsets in tumors and highlight the identification of novel T cell markers with therapeutic or prognostic potential.