bluuebunny/crossref_metadata_2025_split huggingface dataset:
Increased likelihood of heat-induced large wildfires in the Mediterranean Basin
Authors: Ruffault Julien , Curt Thomas , Moron Vincent , Trigo Ricardo M. , Mouillot Florent , Koutsias Nikos , Pimont François , Martin-StPaul Nicolas , Barbero Renaud , Dupuy Jean-Luc , Russo Ana , Belhadj-Khedher Chiraz 
Published: August 2020

AbstractWildfire activity is expected to increase across the Mediterranean Basin because of climate change. However, the effects of future climate change on the combinations of atmospheric conditions that promote wildfire activity remain largely unknown. Using a fire-weather based classification of wildfires, we show that future climate scenarios point to an increase in the frequency of two heat-induced fire-weather types that have been related to the largest wildfires in recent years. Heat-induced fire-weather types are characterized by compound dry and warm conditions occurring during summer heatwaves, either under moderate (heatwave type) or intense (hot drought type) drought. The frequency of heat-induced fire-weather is projected to increase by 14% by the end of the century (2071–2100) under the RCP4.5 scenario, and by 30% under the RCP8.5, suggesting that the frequency and extent of large wildfires will increase throughout the Mediterranean Basin.
RLP23 is required for Arabidopsis immunity against the grey mould pathogen Botrytis cinerea
Authors: Ono Erika , Mise Kazuyuki , Takano Yoshitaka 
Published: August 2020

AbstractNecrosis- and ethylene-inducing-like proteins (NLPs) are secreted by fungi, oomycetes and bacteria. Conserved nlp peptides derived from NLPs are recognized as pathogen-associated molecular patterns (PAMPs), leading to PAMP-triggered immune responses. RLP23 is the receptor of the nlp peptides in Arabidopsis thaliana; however, its actual contribution to plant immunity is unclear. Here, we report that RLP23 is required for Arabidopsis immunity against the necrotrophic fungal pathogen Botrytis cinerea. Arabidopsis rlp23 mutants exhibited enhanced susceptibility to B. cinerea compared with the wild-type plants. Notably, microscopic observation of the B. cinerea infection behaviour indicated the involvement of RLP23 in pre-invasive resistance to the pathogen. B. cinerea carried two NLP genes, BcNEP1 and BcNEP2; BcNEP1 was expressed preferentially before/during invasion into Arabidopsis, whereas BcNEP2 was expressed at the late phase of infection. Importantly, the nlp peptides derived from both BcNEP1 and BcNEP2 induced the production of reactive oxygen species in an RLP23-dependent manner. In contrast, another necrotrophic fungus Alternaria brassicicola did not express the NLP gene in the early infection phase and exhibited no enhanced virulence in the rlp23 mutants. Collectively, these results strongly suggest that RLP23 contributes to Arabidopsis pre-invasive resistance to B. cinerea via NLP recognition at the early infection phase.
Netting and pan traps fail to identify the pollinator guild of an agricultural crop
Authors: Boyer K. J. , Fragoso F. P. , Dieterich Mabin M. E. , Brunet J. 
Published: August 2020

AbstractPollinator decline is expected to cause significant reductions in food production and plant reproduction. Quantifying the impact of pollinator decline on food production requires survey methods that can identify insect and bee species responsible for pollination of specific crops. To address this issue, we compared the effectiveness of two survey methods, netting and pan traps, at capturing the pollinators of alfalfa, Medicago sativa. Alfalfa is a major component of forage for cows and an important ingredient in chicken feed. We also examined bee species richness and diversity with these two survey methods, and compared these measures among three different colors of pan traps. Netting was more effective at capturing known pollinators of alfalfa, especially those belonging to the Bombus and Apis genera. Pan traps captured a higher bee diversity relative to netting and, like previous studies, each survey method and each trap color was more efficient at capturing certain bee genera. However, without a priori knowledge of pollinators, neither survey method could identify which of the bee species captured could pollinate alfalfa. We therefore recommend direct observations when the goal of a study is to identify pollinators or link pollinator decline to food production.
Bacterial microbiota diversity and composition in red and white wines correlate with plant-derived DNA contributions and botrytis infection
Authors: Bubeck Alena M. , Preiss Lena , Jung Anna , Dörner Elisabeth , Podlesny Daniel , Kulis Marija , Maddox Cynthia , Arze Cesar , Zörb Christian , Merkt Nikolaus , Fricke W. Florian 
Published: August 2020

AbstractWine is a globally produced, marketed and consumed alcoholic beverage, which is valued for its aromatic and qualitative complexity and variation. These properties are partially attributable to the bacterial involvement in the fermentation process. However, the organizational principles and dynamic changes of the bacterial wine microbiota remain poorly understood, especially in the context of red and white wine variations and environmental stress factors. Here, we determined relative and absolute bacterial microbiota compositions from six distinct cultivars during the first week of fermentation by quantitative and qualitative 16S rRNA gene amplification and amplicon sequencing. All wines harboured complex and variable bacterial communities, with Tatumella as the most abundant genus across all batches, but red wines were characterized by higher bacterial diversity and increased relative and absolute abundance of lactic and acetic acid bacteria (LAB/AAB) and bacterial taxa of predicted environmental origin. Microbial diversity was positively correlated with plant-derived DNA concentrations in the wine and Botrytis cinerea infection before harvest. Our findings suggest that exogenous factors, such as procedural differences between red and white wine production and environmental stress on grape integrity, can increase bacterial diversity and specific bacterial taxa in wine, with potential consequences for wine quality and aroma.
Morphological and genome-wide evidence for natural hybridisation within the genus Stipa (Poaceae)
Authors: Baiakhmetov Evgenii , Nowak Arkadiusz , Gudkova Polina D. , Nobis Marcin 
Published: August 2020

AbstractHybridisation in the wild between closely related species is a common mechanism of speciation in the plant kingdom and, in particular, in the grass family. Here we explore the potential for natural hybridisation inStipa(one of the largest genera in Poaceae) between genetically distant species at their distribution edges in Mountains of Central Asia using integrative taxonomy. Our research highlights the applicability of classical morphological and genome reduction approaches in studies on wild plant species. The obtained results revealed a new nothospecies,Stipa × lazkovii, which exhibits intermediate characters toS. kryloviiandS. bungeana. A high-density DArTseq assay disclosed thatS. × lazkoviiis an F1 hybrid, and established that the plastid and mitochondrial DNA was inherited fromS. bungeana. In addition, molecular markers detected a hybridisation event between morphologically and genetically distant speciesS. bungeanaand probablyS. glareosa. Moreover, our findings demonstrated an uncertainty on the taxonomic status ofS. bungeanathat currently belongs to the sectionLeiostipa, but it is genetically closer toS. breviflorafrom the sectionBarbatae. Finally, we noticed a discrepancy between the current molecular data with the previous findings onS. capillataandS. sareptana.
The Oligo–Miocene closure of the Tethys Ocean and evolution of the proto-Mediterranean Sea
Authors: Torfstein Adi , Steinberg Josh 
Published: August 2020

AbstractThe tectonically driven Cenozoic closure of the Tethys Ocean invoked a significant reorganization of oceanic circulation and climate patterns on a global scale. This process culminated between the Mid Oligocene and Late Miocene, although its exact timing has remained so far elusive, as does the subsequent evolution of the proto-Mediterranean, primarily due to a lack of reliable, continuous deep-sea records. Here, we present for the first time the framework of the Oligo–Miocene evolution of the deep Levant Basin, based on the chrono-, chemo- and bio- stratigraphy of two deep boreholes from the Eastern Mediterranean. The results reveal a major pulse in terrigeneous mass accumulation rates (MARs) during 24–21 Ma, reflecting the erosional products of the Red Sea rifting and subsequent uplift that drove the collision between the Arabian and Eurasian plates and the effective closure of the Indian Ocean-Mediterranean Seaway. Subsequently, the proto-Mediterranean experienced an increase in primary productivity that peaked during the Mid-Miocene Climate Optimum. A region-wide hiatus across the Serravallian (13.8–11.6 Ma) and a crash in carbonate MARs during the lower Tortonian reflect a dissolution episode that potentially marks the earliest onset of the global middle to late Miocene carbonate crash.
Regulation of colonic epithelial cell homeostasis by mTORC1
Authors: Kotani Takenori , Setiawan Jajar , Konno Tasuku , Ihara Noriko , Okamoto Saki , Saito Yasuyuki , Murata Yoji , Noda Tetsuo , Matozaki Takashi 
Published: August 2020

AbstractCell signaling important for homeostatic regulation of colonic epithelial cells (CECs) remains poorly understood. Mammalian target of rapamycin complex 1 (mTORC1), a protein complex that contains the serine-threonine kinase mTOR, mediates signaling that underlies the control of cellular functions such as proliferation and autophagy by various external stimuli. We here show that ablation of tuberous sclerosis complex 2 (Tsc2), a negative regulator of mTORC1, specifically in intestinal epithelial cells of mice resulted in increased activity of mTORC1 of, as well as increased proliferative activity of, CECs. Such Tsc2 ablation also reduced the population of Lgr5-positive colonic stem cells and the expression of Wnt target genes in CECs. The stimulatory phosphorylation of the kinase Akt and inhibitory phosphorylation of glycogen synthase kinase 3β were both markedly decreased in the colon of the Tsc2 conditional knockout (CKO) mice. Development of colonic organoids with cryptlike structures was enhanced for Tsc2 CKO mice compared with control mice. Finally, Tsc2 CKO mice manifested increased susceptibility to dextran sulfate sodium–induced colitis. Our results thus suggest that mTORC1 activity promotes the proliferation of, as well as the expression of Wnt target genes in, CECs and thereby contributes to colonic organogenesis and homeostasis.
Recapitulation of normal collagen architecture in embryonic wounded corneas
Authors: Koudouna Elena , Spurlin James , Babushkina Anna , Quantock Andrew J. , Jester James V. , Lwigale Peter 
Published: August 2020

AbstractWound healing is characterized by cell and extracellular matrix changes mediating cell migration, fibrosis, remodeling and regeneration. We previously demonstrated that chick fetal wound healing shows a regenerative phenotype regarding the cellular and molecular organization of the cornea. However, the chick corneal stromal structure is remarkably complex in the collagen fiber/lamellar organization, involving branching and anastomosing of collagen bundles. It is unknown whether the chick fetal wound healing is capable of recapitulating this developmentally regulated organization pattern. The purpose of this study was to examine the three-dimensional collagen architecture of wounded embryonic corneas, whilst identifying temporal and spatial changes in collagen organization during wound healing. Linear corneal wounds that traversed the epithelial layer, Bowman´s layer, and anterior stroma were generated in chick corneas on embryonic day 7. Irregular thin collagen fibers are present in the wounded cornea during the early phases of wound healing. As wound healing progresses, the collagen organization dramatically changes, acquiring an orthogonal arrangement. Fourier transform analysis affirmed this observation and revealed that adjacent collagen lamellae display an angular displacement progressing from the epithelium layer towards the endothelium. These data indicate that the collagen organization of the wounded embryonic cornea recapitulate the native macrostructure.
Synthesis and characterization of partially silane-terminated polyurethanes reinforced with acid-treated halloysite nanotubes for transparent armour systems
Authors: Aguiar Rafaela , Miller Ronald E. , Petel Oren E. 
Published: August 2020

AbstractIn the present work, nanocomposites based on the partially silane-terminated polyurethanes reinforced with sulfuric acid-treated halloysite nanotubes were synthesized and evaluated as a potential candidate for transparent blast resistant configurations. The polyurethane must present high tensile ductility at high strain rates to be able to contain fragments and increase the survivability of the system. Gas-gun spall experiments were conducted to measure the dynamic tensile strength (spall strength) and fracture toughness of the nanocomposite and neat polyurethane. The nanocomposite presented a 35% higher spall strength and 21% higher fracture toughness compared to the neat polyurethane while maintaining transparency. The recovered samples following the spall tests were analysed via scanning electron microscope fractographies. The nanocomposite and neat polyurethane samples were chemically characterized via Fourier transform infrared spectroscopy and melting behaviour via differential scanning calorimetry. The improved properties can be attributed, in large part, to the presence of more rigid spherulitic structures, and a rougher fracture surface constituting of several micro-cracks within the nanocomposite.
Unique molecular signatures of antiviral memory CD8+ T cells associated with asymptomatic recurrent ocular herpes
Authors: Prakash Swayam , Roy Soumyabrata , Srivastava Ruchi , Coulon Pierre-Gregoire , Dhanushkodi Nisha R. , Vahed Hawa , Jankeel Allen , Geertsema Roger , Amezquita Cassandra , Nguyen Lan , Messaoudi Ilhem , Burkhardt Amanda M. , BenMohamed Lbachir 
Published: August 2020

AbstractThe nature of antiviral CD8+ T cells associated with protective and pathogenic herpes simplex virus type 1 (HSV-1) infections remains unclear. We compared the transcriptome, phenotype, and function of memory CD8+ T cells, sharing the same HSV-1 epitope-specificities, from infected HLA-A*0201 positive symptomatic (SYMP) vs. asymptomatic (ASYMP) individuals and HLA-A*0201 transgenic rabbits. Compared to higher frequencies of multifunctional effector memory CD8+ TEM cells in ASYMP individuals, the SYMP individuals presented dysfunctional CD8+ TEM cells, expressing major exhaustion pathways. Compared to protected ASYMP HLA transgenic rabbits, the trigeminal ganglia of non-protected SYMP HLA transgenic rabbits had higher frequencies of dysfunctional tissue-resident CD8+ TRM cells. Moreover, blockade of T cell exhaustion pathways restored the function of CD8+ T cells, reduced virus reactivation, and diminished recurrent disease in HLA transgenic rabbits. These findings reveal unique molecular signatures of protective CD8+ T cells and pave the way for T-cell-based immunotherapy to combat recurrent ocular herpes.
Strategies for rapid reconstruction in 3D MRI with radial data acquisition: 3D fast Fourier transform vs two-step 2D filtered back-projection
Authors: Park Jinil , Lee Jeongtaek , Lee Joonyeol , Lee Seung-Kyun , Park Jang-Yeon 
Published: August 2020

AbstractFor 3D radial data reconstruction in magnetic resonance imaging (MRI), fast Fourier transform via gridding (gFFT) is widely used for its fast processing and flexibility. In comparison, conventional 3D filtered back projection (cFBP), while more robust against common radial k-space centering errors, suffers from long computation times and is less frequently used. In this study, we revisit another back-projection reconstruction strategy, namely two-step 2D filtered back-projection (tsFBP), as an alternative 3D radial MRI reconstruction method that combines computational efficiency and certain error tolerance. In order to compare the three methods (gFFT, cFBP, and tsFBP), theoretical analysis was performed to evaluate the number of computational steps involved in each method. Actual reconstruction times were also measured and compared using 3D radial-MRI data of a phantom and a human brain. Additionally, the sensitivity of tsFBP to artifacts caused by radial k-space centering errors was compared with the other methods. Compared to cFBP, tsFBP dramatically improved the reconstruction speed while retaining the benefit of tolerance to the radial k-space errors. Our study therefore suggests that tsFBP can be a promising alternative to the conventional back projection method for 3D radial MRI reconstruction.
3D particle transport in multichannel microfluidic networks with rough surfaces
Authors: Ryan Duncan P. , Chen Yu , Nguyen Phong , Goodwin Peter M. , Carey J. William , Kang Qinjun , Werner James H. , Viswanathan Hari S. 
Published: August 2020

AbstractThe transport of particles and fluids through multichannel microfluidic networks is influenced by details of the channels. Because channels have micro-scale textures and macro-scale geometries, this transport can differ from the case of ideally smooth channels. Surfaces of real channels have irregular boundary conditions to which streamlines adapt and with which particle interact. In low-Reynolds number flows, particles may experience inertial forces that result in trans-streamline movement and the reorganization of particle distributions. Such transport is intrinsically 3D and an accurate measurement must capture movement in all directions. To measure the effects of non-ideal surface textures on particle transport through complex networks, we developed an extended field-of-view 3D macroscope for high-resolution tracking across large volumes ($$25\,\hbox {mm} \times 25\,\hbox {mm} \times 2\,\hbox {mm}$$

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) and investigated a model multichannel microfluidic network. A topographical profile of the microfluidic surfaces provided lattice Boltzmann simulations with a detailed feature map to precisely reconstruct the experimental environment. Particle distributions from simulations closely reproduced those observed experimentally and both measurements were sensitive to the effects of surface roughness. Under the conditions studied, inertial focusing organized large particles into an annular distribution that limited their transport throughout the network while small particles were transported uniformly to all regions.
Chemogenomic screening identifies the Hsp70 co-chaperone DNAJA1 as a hub for anticancer drug resistance
Authors: Nitika  , Blackman Jacob S. , Knighton Laura E. , Takakuwa Jade E. , Calderwood Stuart K. , Truman Andrew W. 
Published: August 2020

AbstractHeat shock protein 70 (Hsp70) is an important molecular chaperone that regulates oncoprotein stability and tumorigenesis. However, attempts to develop anti-chaperone drugs targeting molecules such as Hsp70 have been hampered by toxicity issues. Hsp70 is regulated by a suite of co-chaperone molecules that bring “clients” to the primary chaperone for efficient folding. Rather than targeting Hsp70 itself, here we have examined the feasibility of inhibiting the Hsp70 co-chaperone DNAJA1 as a novel anticancer strategy. We found DNAJA1 to be upregulated in a variety of cancers, suggesting a role in malignancy. To confirm this role, we screened the NIH Approved Oncology collection for chemical-genetic interactions with loss of DNAJA1 in cancer. 41 compounds showed strong synergy with DNAJA1 loss, whereas 18 dramatically lost potency. Several hits were validated using a DNAJA1 inhibitor (116-9e) in castration-resistant prostate cancer cell (CRPC) and spheroid models. Taken together, these results confirm that DNAJA1 is a hub for anticancer drug resistance and that DNAJA1 inhibition is a potent strategy to sensitize cancer cells to current and future therapeutics. The large change in drug efficacy linked to DNAJA1 suggests a personalized medicine approach where tumor DNAJA1 status may be used to optimize therapeutic strategy.
Multifunctional light beam control device by stimuli-responsive liquid crystal micro-grating structures
Authors: Algorri J. F. , Morawiak P. , Zografopoulos D. C. , Bennis N. , Spadlo A. , Rodríguez-Cobo L. , Jaroszewicz L. R. , Sánchez-Pena J. M. , López-Higuera J. M. 
Published: August 2020

AbstractThere is an increasing need to control light phase with tailored precision via simple means in both fundamental science and industry. One of the best candidates to achieve this goal are electro-optical materials. In this work, a novel technique to modulate the spatial phase profile of a propagating light beam by means of liquid crystals (LC), electro-optically addressed by indium-tin oxide (ITO) grating microstructures, is proposed and experimentally demonstrated. A planar LC cell is assembled between two perpendicularly placed ITO gratings based on microstructured electrodes. By properly selecting only four voltage sources, we modulate the LC-induced phase profile such that non-diffractive Bessel beams, laser stretching, beam steering, and 2D tunable diffraction gratings are generated. In such a way, the proposed LC-tunable component performs as an all-in-one device with unprecedented characteristics and multiple functionalities. The operation voltages are very low and the aperture is large. Moreover, the device operates with a very simple voltage control scheme and it is lightweight and compact. Apart from the demonstrated functionalities, the proposed technique could open further venues of research in optical phase spatial modulation formats based on electro-optical materials.
Bone metastasis is associated with acquisition of mesenchymal phenotype and immune suppression in a model of spontaneous breast cancer metastasis
Authors: Monteran Lea , Ershaid Nour , Sabah Idan , Fahoum Ibrahim , Zait Yael , Shani Ophir , Cohen Noam , Eldar-Boock Anat , Satchi-Fainaro Ronit , Erez Neta 
Published: August 2020

AbstractThe most common site of breast cancer metastasis is the bone, occurring in approximately 70% of patients with advanced disease. Bone metastasis is associated with severe morbidities and high mortality. Therefore, deeper understanding of the mechanisms that enable bone-metastatic relapse are urgently needed. We report the establishment and characterization of a bone-seeking variant of breast cancer cells that spontaneously forms aggressive bone metastases following surgical resection of primary tumor. We characterized the modifications in the immune milieu during early and late stages of metastatic relapse and found that the formation of bone metastases is associated with systemic changes, as well as modifications of the bone microenvironment towards an immune suppressive milieu. Furthermore, we characterized the intrinsic changes in breast cancer cells that facilitate bone-tropism and found that they acquire mesenchymal and osteomimetic features. This model provides a clinically relevant platform to study the functional interactions between breast cancer cells and the bone microenvironment, in an effort to identify novel targets for intervention.