bluuebunny/crossref_metadata_2025_split huggingface dataset:
Study on Functional Monoclonal Antibodies of Anti-Human Uterine Sarcoma Stem Cell-Like Cells
Authors: Cai Jingting , Gao Jiuping , Zhang Beilei , Tang Dihong , Gao Ting , Lin Qiuhui , Tang Jun , Chu Chaonan , Yang Ting , Wang Jing , Wang Jing 
Published: March 2020

Abstract
Background: To establish a functional monoclonal antibody library using Human Uterine Sarcoma Stem Cell-Like Cells (HUSSLCs) to screen and identify functional monoclonal antibodies that can recognize and inhibit HUSSLCs. Methods: B lymphocytes in proliferative state were prepared by using the second generation CD133+spheroid cells of SK-UT-1 cell line, i.e. HUSSLCs, as antigens; Spheroid formation, agar colony formation, wound healing, flow cytometry, and Western blotting were adopted to detect the effect of monoclonal antibodies with varied dilution ratios on HUSSLCs spheroid formation, agar colony formation, cell migration, CD133 expression, and expression of CD44, ABCG2, Bmi1, Nanog, Oct4 and ALDH1. Results: Myeloma cells of SP2/0 cell line can achieve 85% degrees of fusion and results of 1-2F monoclonal cell supernatants with different dilution ratios reduced HUSSLCs spheroid formation rate, agar colony formation rate, cell migration rate, CD133 positive cell expression and protein expression levels of CD44, ABCG2, Bmi1, Nanog, Oct4, and ALDH1 in concentration-dependent manner (P <0.05). Conclusion: The antibody valence produced by HUSSLCs-immunized mice reached the requirement for preparation of monoclonal antibody. Anti-HUSSLCs monoclonal antibodies feature functions of inhibiting the self-renewal, unrestricted proliferation, migration, invasion and multidrug resistance of HUSSLCs and functions characterized by tumor stem cells. Key words: uterine leiomyosarcoma; tumor stem cell; monoclonal antibody; self-renewal ability; infinite multiplication; drug resistance
Knockdown of lncRNA LINC01234 suppresses the tumorigenesis of liver cancer via sponging miR-513a-5p
Authors: Xu Wen , Li Kesang , Song Changfeng , Wang Xiaotong , Li Yueqi , Bai Xue , Liang Xin , Deng Wanli , Wang Junqing , Liu Jianwen 
Published: March 2020

Abstract
Background Liver cancer is a frequent malignancy with poor prognosis. It has been reported that many lncRNAs could regulate the progression of liver cancer. To identify potential therapeutic targets for liver cancer, we conducted bioinformatics analysis of lncRNAs in tumor tissues and adjacent normal tissues. Methods The differential expression of lncRNAs between liver cancer tissues and adjacent normal tissues were examined by bioinformatics analysis. Cell proliferation was tested by CCK-8. Cell apoptosis in liver cancer was detected by flow cytometry. Gene and protein expression in liver cancer cells were measured by q-PCR and Western-blot, respectively. Xenograft tumor model was established to verify the function of LNC01234 on liver cancer in vivo. Results LNC01234 was found to be notably upregulated in liver cancer tissues. In addition, knockdown of LNC01234 significantly inhibited the proliferation, invasion and induced the apoptosis of liver cancer cells. Meanwhile, miR-513a-5p was a downstream target of LNC01234 and USP4 was a direct target of miR-513a-5p. Moreover, downregulation of LNC01234 inhibited the tumorigenesis of liver cancer via inactivating TGF-β signaling. Conclusion Downregulation of LNC01234 could inhibit the progression of liver cancer, which may serve as a potential novel target for treatment of liver cancer.
Contemporary management of brain arteriovenous malformations in mainland China: a web-based nationwide questionnaire survey
Authors: Yu Chen , Meng Xiangyu , Ma Li , Zhao Yang , Gu Ye , Jin Hengwei , Gao Dezhi , Li Youxiang , Sun Shibin , Liu Ali , Zhao Yuanli , Chen Xiaolin , Wang Shuo 
Published: March 2020

Abstract
Background:In the benefit of the large population and rapid economic growth, the interventional techniques and equipment for brain arteriovenous malformations (bAVMs) in mainland China have been rapidly improved. Chinese neurosurgical cerebrovascular physicians have accumulated rich experience and made pioneering explorations. This study aims to summarize the experience and treatment progress of bAVMs in mainland China.Methods:We performed a web-based nationwide questionnaire survey among 67 tertiary neurosurgical institutions that had acknowledged treating bAVMs in the primary survey. Our questionnaire included clinical characteristics, radiological findings, intervention indications/contraindications, intervention timing, and intraoperative management of different treatment modalities. Results:A total of 63 participants from 49 (73.1%) tertiary neurosurgical institutions responded to our questionnaire. 42 (66.7%) were neurosurgeons, 13 (20.6%) were neurointerventionists, and 8 (12.7%) were radiosurgeons. Approximately 3500 to 4000 cases of bAVMs were treated annually in these 49 departments. All participants agreed that the conclusions of ARUBA are debatable. Flow-related aneurysms, deep venous drainage, and arteriovenous fistula were considered as common hemorrhagic risk factors. Unruptured SM IV-V bAVMs, giant bAVMs, pediatric bAVMs, elderly bAVMs, and eloquent bAVMs were not absolute contraindications to intervention. Maximum lesion occlusion and minimal functional impairment were the principles of intervention management. Most of the neurosurgeons and neurointerventionists recommended early intervention (<30 days) for ruptured bAVMs, and the radiosurgeons suggested intervention in the chronic phase or recovery phase (P<0.01), and preferably 3 months after bleeding. Multi-modality strategies were thought effective for complex bAVMs and more exploration of individualized intraoperative management was necessary.Conclusions:Intervention was acceptable for specific selected unruptured bAVMs in mainland China, especially in patients with hemorrhagic risk factors. The application of multidisciplinary cerebrovascular team and multicenter large-sample international registry study might be the next work for Chinese neurosurgical cerebrovascular physicians.
Obstacle avoidance and multitarget tracking of a super redundant modular manipulator based on Bezier curve and particle swarm optimization
Authors: Li Chen , Ma Ying , Zhang Yu , Liu Jinguo 
Published: March 2020

Abstract
A super redundant serpentine manipulator has slender structure and multiple degrees of freedom and can travel through narrow space and move in complex space. This manipulator is composed of many modules that can form different lengths of robot arms for different application sites. The increase in degrees of freedom causes the inverse kinematics of redundant manipulator to be typical and immensely increases the calculation load in the joint space. This paper presents a composite optimization method of path planning for obstacle avoidance and discrete trajectory tracking of a super redundant manipulator. In this composite optimization, path planning is established on a Bezier curve, particle swarm optimization is adopted to adjust the control points of the Bezier curve with the kinematic constraints of manipulator, and a feasible obstacle avoidance path is obtained along with a discrete trajectory tracking using a follow-the-leader strategy. The relative distance between each two discrete path points is limited to reduce the fitting error of the connecting rigid links to the smooth curve. Simulation results show that this composite optimization method can rapidly search for the appropriate trajectory to guide the manipulator in obtaining the target while achieving obstacle avoidance and meeting joint constraints. The proposed algorithm is suitable for 3D space obstacle avoidance and multitarget path tracking.
Finite Cell Method for functionally graded materials based on V-models and homogenized microstructures
Authors: Wassermann Benjamin , Korshunova Nina , Kollmannsberger Stefan , Rank Ernst , Elber Gershon 
Published: March 2020

Abstract
This paper proposes a computational methodology that allows a direct numerical simulation of heterogeneous/functionally graded materials based on V-reps/V-models and the Finite Cell Method (FCM). The FCM is an embedded domain approach that employs higher-order ﬁnite elements. The basic idea is to embed a complex geometric model into a ﬁctitious domain that is trivial to mesh. The complexity of the geometry is then recaptured by an adapted precise numerical integration scheme for the elements cut by the boundary. For this, only a robust point inclusion test is required, which can be provided by various Computer-Aided Design (CAD) models. V-rep is a geometric modeling framework that represents the entire volume based on tri-variate B-Splines. Consequently, not only a point inclusion test is provided – but also the possibility to represent and model the interior domain. This allows to apply functionally graded material based on the tri-variate basis functions. These material parameters can then be regained during the simulation with an adapted point inclusion test. The potential of the proposed method especially in the context of additive manufacturing is demonstrated by several numerical examples.
Transduction of inflammation from peripheral immune cells to the hippocampus induces neuronal hyperexcitability mediated by Caspase-1 activation
Authors: Shaker Tarek , Chattopadhyaya Bidisha , Amilhon Bénédicte , Cristo Graziella Di , Weil Alexander G. 
Published: March 2020

Abstract
Background Recent studies report infiltration of peripheral blood mononuclear cells (PBMCs) into the central nervous system (CNS) in epileptic disorders, suggestive of a potential contribution of PBMC extravasation to the generation of seizures. Nevertheless, the underlying mechanisms involved in PBMC infiltrates promoting neuronal predisposition to ictogenesis remain unclear. Therefore, we developed an in vitro model mimicking PBMC infiltration into the brain in order to investigate potential transduction of inflammatory signals from PBMCs to the CNS.MethodsTo establish our model, we first extracted PBMCs from rat spleen, then, we immunologically primed PBMCs with lipopolysaccharide (LPS), followed by immunological activation with Nigericin. Thereafter, we cultured PBMCs on top of organotypic cortico-hippocampal brain slice cultures (OCHSCs) derived from the same rat, and compared PBMC-OCHSC co-cultures to OCHSCs exposed to PBMCs in the culture media. Also, we targeted a potential molecular pathway underlying transduction of peripheral inflammation to OCHSCs by incubating OCHSCs with the Caspase-1 inhibitor VX-765 prior to co-culturing PBMCs with OCHSCs. After 24 hours, we immunohistochemically analyzed inflammation markers in the cortex and the hippocampus. In addition, we performed whole-cell patch-clamp recordings in cortical layer II/III and hippocampal CA1 pyramidal neurons.ResultsIn the cortex, co-culturing immunoreactive PBMCs treated with LPS + Nigericin on top of OCHSCs induced ectopic expression of inflammation markers and enhanced neuronal excitation. In contrast, no excitability changes were detected after adding primed PBMCs, i.e. treated with LPS only, to OCHSCs. Strikingly, in the hippocampus, both immunoreactive and primed PBMCs elicited similar pro-inflammatory and pro-excitatory effects. However, when immunoreactive and primed PBMCs were cultured in the media separately from OCHSCs, only immunoreactive PBMCs gave rise to neuroinflammation and hyperexcitability in the hippocampus, whereas primed PBMCs failed to produce any significant changes. Finally, VX-765 application to OCHSCs, co-cultured with either immunoreactive or primed PBMCs, protected them from neuroinflammation and hippocampal hyperexcitability.ConclusionsOur study shows a higher susceptibility of the hippocampus to peripheral inflammation as compared to the cortex, mediated via Caspase-1-dependent signaling pathways. Thus, our findings suggest that Caspase-1 inhibition may potentially provide therapeutic benefits during hippocampal neuroinflammation and hyperexcitability secondary to peripheral innate immunity.
Evaluating the predictive value of procalcitonin, cough duration, and heat peak in severe Mycoplasma pneumoniae pneumonia
Authors: Yang Ying , Chen Liqing , Liu Yan 
Published: March 2020

Abstract
Objective To investigate the predictive value of risk factors for severe Mycoplasma pneumoniae pneumonia (MPP). Methods Retrospective analyses were conducted on 342 children with acute MPP hospitalized between December 2015 and December 2018. Results A total of 342 cases were including in the analyses, with most cases involving infants and preschool-aged children. Patients were categorized as general and severe MPP. Patients in the severe group had increased heat peak, fever duration, cough duration, length of hospitalization, and levels of white blood cell, neutrophils, C-reactive protein, and procalcitonin, as well as reduced levels of lymphocytes and platelets compared to the general group. Logistic regression and receiver operating characteristic curve analyses showed that increased levels of procalcitonin (>0.67 ng/mL), prolonged cough duration (>13.5 days), and heat peak above 38.4°C may be early predictors for severe MPP. Conclusions The severity of MPP effects fever, cough duration, and inflammatory response. Taking a detailed medical history and early procalcitonin measurement may help detect severe MPP.
Associations between participation in, intensity of, and time spent on leisure time physical activity and risk of inflammatory bowel disease among older adults (PA-IBD): A prospective cohort study
Authors: Rasmussen Nathalie Fogh , Bech Bodil Hammer , Rubin Katrine Hass , Andersen Vibeke 
Published: March 2020

Abstract
Background

Inflammatory bowel diseases (IBDs) are diseases of the immune system that share some genetic and lifestyle-related predisposing factors. Increasing incidences have been reported in all age groups. Based on experimental studies suggesting a role of physical activity on intestinal inflammation, this study aimed to investigate the association between leisure time physical activity and the risk of IBD in older adults.

Methods

The study is a prospective cohort study using Danish registry data and questionnaire data from the Danish “Diet, Cancer and Health” cohort. The outcome IBD was defined as having at least two diagnoses of Crohn’s disease or ulcerative colitis registered in the National Patient Registry during follow-up between December 1993 and May 1997 until December 2018. Cox proportional hazard models were used to estimate hazard ratios for IBD onset associated with being physically active and with levels of the metabolic equivalent of task (MET) hours/week of physical activity and hours/week spent on six types of physical activity.

Results

In total, 54 645 men and women aged 50-64 years were included, and thereof 529 cases. When comparing physically active with inactive participants measured by MET hours/week there was no statistically significant difference in risk of IBD (0.89 [0.13; 6.27]), neither when measured as participation in six types of activities. Results did not indicate any dose-response effect when comparing quartile groups of MET hours/week or of five of the six types of activities. For do-it-yourself-work, the third quartile of hours/week was associated with a higher risk of IBD compared to the second quartile (HR=1.44 [1.10 ; 1.90]. No effect modification was found.

Conclusions

There was no association between physical activity and risk of IBD when comparing physically active with inactive participants. Neither did the results indicate any dose-response effect when comparing quartile groups of MET hours/week. Do-it-yourself work, however, seemed to be associated with a higher risk of IBD when comparing the third quartile with the second quartile. The study has clinical relevance by its contribution to the explanatory field of the causes of IBD. However, further research is needed to clarify associations between physical activity and risk of IBD.
Statistical modeling of number of human deaths per road traffic accident in Oromia region, Ethiopia
Authors: Aga Merga Abdissa , Woldeamanuel Berhanu Teshome , Tadesse Mekonnen 
Published: March 2020

Abstract
Abstract

Background : Globally, road traffic accidents are leading causes of death among young people in general, and the main cause of death among young people aged 15–29 years. This study was conducted to identify the major factors associated with the number of human deaths by road traffic accident in the Oromia Regional State, Ethiopia.
Methods: We used data obtained from the Oromia Police Commission Bureau that have been recorded on daily basis road traffic accidents from July, 2016 - July, 2017. Count regression models were was used to assess the factors associated with the number of human deaths from traffic accidents.
Results : Of the total of 3900 road traffic accidents included in the 1188 (30.5%) were giving rise to fatal. The Hurde models were of better fit than zero inflated Poisson and zero inflated negative binomial model. Thus, the Hurdle Poisson is recommended in this study. Age of the driver 31-50 years (AOR = 0.289, 95%CI: 0.175, 0.479) and higher than 50 years of age (AOR = 0.311, 95%CI: 0.129, 0.751), driver’s years of experience 5-10 years (AOR = 0.014, 95%CI: 0.007, 0.027), and more than 10 years (AOR = 0.101, 95%CI: 0.057, 0.176), vehicle type automobile (AOR = 8.642, 95%CI: 2.7644, 27.023), vehicle years of service 5-10 years (AOR = 2.484, 95%CI: 1.194, 5.169), and more than 10 years (AOR = 2.639, 95%CI: 1.268, 5.497), type of accident, vehicle upside down (AOR = 5.560, 95%CI: 2.506, 12.336), causes of accident, turning illegal position (AOR = 0.454, 95%CI: 0.226, 0.913), area of accident, residential place (AOR = 108.506, 95%CI: 13.725, 857.798), working areas (AOR = 129.606, 95%CI: 16.448, 1021.263), near hospitals (AOR = 23.789, 95%CI: 3.038, 186.298), geographical locations, Western zones (AOR = 0.275, 95%CI: 0.167, 0.455), and South east zones (AOR = 0.624, 95%CI: 0.410, 0.950) were significant associated number of human deaths per road traffic accident factors in the study area.
Conclusion :
In this study, 30.5% of accidents were giving rise to at least one human death per road traffic accident and different associated numbers of human deaths per traffic accident factors have been identified.
Closed-loop target-controlled infusion system for propofol guided by bispectral index applied in patients undergoing shoulder arthroscopy in the beach chair position- a randomized controlled trial
Authors: Zhao Zhao , Li Junjie , Fang Yang , Nanbo Luo , Han Yang , Xinping Yang , Liangquan Peng , Wei Lu , Fang Guangguang , Daping Wang , Liu Zhiheng 
Published: March 2020

Abstract
Background To compare the efficacy of anesthetic depth control using closed-loop and open-loop target controlled infusion (TCI) system of propofol guided by BIS in patients undergoing shoulder arthroscopy in the beach chair position (BCP). Methods 120 patients underwent shoulder arthroscopy surgery in the BCP were randomized into two groups, the open-loop (O) group and the closed-loop (C) group. During the maintenance phase, BIS value was used as the feedback variable for TCI system of propofol in both groups. The Global score (GS) and the percentage of adequate anesthesia, the frequency of propofol regulation, and consumption of propofol were calculated. The MMSE scores of the day before and 1 day after surgery, serum GFAP and S100B proteins before anesthesia, after extubation and 1 day after surgery were compared. Results The GS and the proportion of appropriate anesthesia time were better in the group C. The percentage of overshoot time was lower in the group C. The frequency of propofol regulation was observed higher in the group C. Propofol consumption in the group C was significantly lower than that in the group O. The MMSE scores, the GFAP and S100B protein concentrations had no significant difference between the two groups. Conclusion Propofol administration using close-loop TCI system guided by BIS may increase the percentage of adequate anesthesia and shorten the percentage of overshoot time compared with open-loop TCI model in anesthesia maintenance phase in patients undergoing shoulder arthroscopy in the BCP, and do not increase the risk of POCD.
A knowledge-based intensity-modulated radiation therapy treatment planning technique for locally advanced nasopharyngeal carcinoma radiotherapy
Authors: Bai Penggang , Weng Xing , Quan Kerun , Chen Jihong , Dai Yitao , Xu Yuanji , Lin Fasheng , Zhong Jing , Wu Tianming , Chen Chuanben 
Published: March 2020

Abstract
Background To investigate the feasibility of a knowledge-based automated intensity-modulated radiation therapy (IMRT) planning technique for locally advanced nasopharyngeal carcinoma (NPC) radiotherapy. Methods 140 NPC patients treated with definitive radiation therapy with the step-and-shoot IMRT techniques were retrospectively selected and consisted of a knowledge library (115 patients) and a test library (25 patients). For each patient in the knowledge library, their overlap volume histogram (OVH), target volume histogram (TVH) and dose objectives were extracted from the patient’s manual treatment plan, and these were used to train a 3 layer neural network (NN) model. The OVH and TVH from the test library were input into the trained model to derive patients’ dose objectives which were subsequently used to generate automated plans (APs) by an in-house developed Perl and HotScripts planning scripts with a single iteration optimization. The corresponding manual plans (MPs) of patients in the test library were manually generated by an experienced medical physicist according to clinical protocols. Plan quality was ranked and dosimetric parameters were compared between the APs and MPs. Results Qualitatively, the APs and MPs had the same rank for the majority of the patients (19 of 25). PTV achieved each given criteria in the majority of the patients (greater than 80%) between both the APs and MPs. For each OAR, the number achieving its criterion in the APs was close to that in the MPs. The APs would improve the treatment delivery efficiency by reducing total plan MUs by ~5% (685.24±58.89 vs. 721.36±63.36, P =0.004). AP also significantly improved planning efficiency by reducing the planning duration to ~17% of the MP (9.73±1.80 min vs. 57.10±6.35 min, P <0.001). Conclusion A robust and effective knowledge-based IMRT treatment planning technique for locally advanced NPC is developed. Patient specific TDV can be predicted by a trained NN model based on the individual’s OVH and clinical TVH goals. The automated planning scripts can use these TDV to generate APs with largely shortened planning time with comparable or improved dosimetric qualities compared to our clinic’s manual plans.