bluuebunny/crossref_metadata_2025_split huggingface dataset:
Assoclation polymorphism apoe gene and blood lipid profile with diffe different types of stroke in Siberia
Authors: Voevoda M. I. , Skurikhina Yu. V. , Shishkin S. V. 
Published: June 2020

The present research aimed at studying association between Apo E polymorphism and certain types of stroke and also association factors of lipid profile’s results and different types of stroke it depends on sex and conditions of living in typical West Siberian megapolis (Novosibirsk).The causes of brain stroke were registered in representative population with population about 150 000 in according to standarts of WHO program MONICA and previous program «Register of stroke». From 2003 to 2007 among new registered and repeated causes of stroke was form at random 15% sample (261 patients). The average age of stroke attack is 61,3 ± 1,0 for men and 61,3 ± 1,0 for women.Independent influence of APOE gene's genotypes polymorphism on development at different stroke patients has not been revealed.
LOCAL AND SYSTEM EFFECTS OF HYALURONIC ACID IMPLANTS IN CONDITIONS OF CORRECTIONS OF AGE-RELATED CHANGES IN SKIN
Authors: Khlusov I. A. , Igumnov V. A. , Choukhnova D. I. , Mytasova O. L. , Zaitsev K. V. , Abdulkina N. G. , Zaitsev A. A. , Kolesova L. Yu. 
Published: June 2020

Local and system influence the Repleri gel implants on the base of hyaluronic acid used to correct age-related changes in skin has been studied. Human stromal stem cells with CD44 membrane receptor to hyaluronic acid migrates in vitro into gel structure and maturates morphologically in fibroblast-like forms stained positively with acid phosphatase. Repleri gels implantation in the group of women with local and system age-related changes in homeostasis has positive local effect connected with full deletion of nasolabial fold in facial skin. Repleri gels injection has been decreasing during 1 year the system indices of real biological age as compared with chronological (passport) one. Lipoproteins and aspartate aminotransferase concentrations diminished statistically in women blood. An effect revealed may be considered as potential way of anti-age therapy of not only skin but parenchymal organs. At the same time, a long-term control of homeostasis distant indices in women with serious changes in parenchymal organs is necessary after local Repleri gels injection because of organism’s hormonal reactions.
HEPATOPROTECTIVE ACTION OF POLAR LIPIDS OF MARAL ANTLERS AND PEAT IN EXPERIMENTAL LIVER DAMAGE CAUSED BY ISONIAZIDE AND PARACETAMOL
Authors: Yatsenkov A. I. 
Published: June 2020

In experimental liver pathology caused by isoniazide or paracetamol administration to albino rats lipids derived from maral antlers and peat decreased the blood activity of aminotransferases, γ-glutamyltranspeptidase, acid and alkaline phosphatases, phospholipase А, content of common bilirubin, activated the detoxication of biliribin, ammonium and phenols, inhibited the liver formation of dienic conjugates, Schiff’s bases, malonic dialdehyde, improve the reduced glutathione function. Maral anthlers and peat lipids in effective doses 30 and 60 mg/kg had the more pronounced hepatoprotective and antioxidant action than lipids in dose 10 mg/kg and essentiale forte N.
DEVELOPMENT OF THE MODELS TO OPTIMIZATION IN SYSTEM BIOTEHNICAL OF THE PLAYING CAR TRAINING
Authors: Pyatakovich F. A. , Yakunchenko T. I. , Makkonen K. F. 
Published: June 2020

The Purpose – its were developed multiparametric models and algorithms of the optimization playing training biooperated. In work are used methods of the system analysis, modeling, system technical analysis and constructing, mathematical statistics, methods of the computer analysis heart rate variability by means of sensor of the pulse and breathings.The Conclusion: its were designed two models of strategy playing, one – with installation on avoid of the failures and the second – on achievement of the success. The algorithms of the success and efficiency were formed for period of the termination of the biooperated play.
ADHD GROUP RISK SCREENING BASED ON GAME BIOFEEDBACK
Authors: Jafarova O. A. , Grebneva O. L. , Stoller I. A. 
Published: June 2020

To organize the systematic selection and formation of risk groups regarding attention disorder, the algorithm of screening diagnostics for primary school students was developed using game biofeedback based on heart rate and time of response to suddenly appearing stimuli (test "Rally"). Proposedareclassificationrules for including the subjects into the risk group, the intermediate group, and the group with satisfactory test results.
RADIONUCLIDE INDICATION OF SENTINEL LYMPH NODES IN LARYNX AND LARYNGOPHARYNX CANCER
Authors: Sinilkin I. G. , Chernov V. I. , Choinzonov Ye. L. , Chizhevskaya S. Yu. , Titskaya A. A. , Zelchan R. V. 
Published: June 2020

Aim: to determine the possibility of radionuclide methods in the identification of "sentinel" lymph node (SLN) in larynx and laryngopharynx cancer patients.Material and methods: 17 patients with larynx and laryngopharynx cancer were included in research. In all patients for SLN visualization nanocolloid radiopharmaceutical was injection around tumor into mucous coat with the subsequent SPECT and radioguided study.Results: Sensitivity and specificity of SPECT and radioguided study for SLN identification were 100%. We found 22 SLN in 17 patients (from 1 to 2 per patient, on average 1.3). Most often SLN were located in the III level of a neck (lymph nodes around of carotid arteries) – 12 SLN (54.5%) and IIA level (under lower jaw lymph nodes) – 6 (27.2%). One SLN (4.5%) was localized in IV level and 3 nodes (13.6%) in VI level. In 2 patients (11.8%) metastasis in SLN were found. In these patients within 2 years cancer progressing was revealed. Fifteen patients (88.2%) had no metastatic in SLN and had no progressing of a cancer.Conclusions: Sensitivity and specificity of SPECT and radioguided study for SLN identification are 100%. In patients with metastatic SLN radical surgery with lymph node dissection is helpful.
ULTRASONIC EXAMINATION OF VENTILATION TUBES PATENCY IN PATIENTS WITH OTITIS MEDIA WITH EFFUSION AFTER TIMPANOSTOMY
Authors: Shcherbik N. V. , Konushkin V. A. , Starokha A. V. , Litvak M. M. 
Published: June 2020

The aim of this study was to improve the accuracy and sensitivity of methods for ventilation tube patency evaluation after timpanostomy. New method of one-dimensional ultrasound middle ear sonography was developed. We studied ventilation tubes patency in 40 patients with otitis media with effusion, after timpanostomy. All patients tympanometry and one-dimensional ultrasound sonography was obtained at 4, 7th and one month after surgery. After timpanostomy in children with the otitis media with effusion, at 4 days after the surgery one-dimensional ultrasound sonography detected normal function of ventilation tubes in 75% patients. At the same time, according to tympanometry ventilation tube patency was recorded in 80% patients. On 7th day after surgery one-dimensional ultrasound sonography detected normal function of ventilation tubes in 85% patients. According to tympanometry the 90% patients demonstrated normal ventilation tube function. One month after surgery one-dimensional ultrasound sonography detected normal function of ventilation tubes in 92.5% patients. At the same time, according to tympa nometry ventilation tube patency was recorded in 95% people. Calculation of ultrasound sonography sensitivity and specificity showed that the sensitivity of this process is 97.4%, specificity – 100%. The statistical significance of differences in ventilation tube patency assessment is not determined. Thus, we have proved one-dimensional ultrasound sonography the sensitive method for ventilation tubes patency validation. The method is simple, requires a bit of time for application, so it can be widely recommended for outpatient network.
THE ROLE OF MICROBIAL COMMUNITIES OF AIRWAYS IN PATHOGENESIS OF CHRONIC OBSTRUCTIVE PULMONARY DISEASE
Authors: Fedosenko S. V. , Ogorodova L. M. , Karnaushkina M. A. , Kulikov Ye. S. , Deyev I. A. , Kirillova N. A. 
Published: June 2020

This review summarizes the results of studies on the composition of microbial communities in the airways of healthy subjects and in patients with chronic obstructive pulmonary disease. Modern technologies of molecular-genetic identification methods of microorganisms allow to perform a deep analysis of the respiratory microbiom. It is of considerable interest to determine the role of the microbiome in the development of human diseases of the bronchopulmonary system, and to understand the impact of the microbes communities as a course of disease and the important factor for the efficacy of current therapy.
EFFECTS OF PHYSICAL EXERCISES ON TRIACYLGLYCEROL LEVEL IN SKELETAL MUSCLES IN DIETARY-INDUCED OBESE RATS
Authors: Yakimovich I. Yu. , Borodin D. A. , Podrezov I. K. , Ivanov V. V. , Vasiliyev V. N. , Kotlovsky M. Yu. 
Published: June 2020

The accumulation of triacylglycerol in peripheral tissues is one of mechanisms of insulin resistance. This paper presents the investigation of the influence of aerobic and anaerobic physical exercises on triacylglycerol level in skeletal muscles and on insulin resistance in dietary-induced obese rats. It is estimated that a high-energy (HE) diet causes the accumulation of triacylglycerols in skeletal muscles that leads to high resistance to insulin. Aerobic and anaerobic physical exercises reduce the level of triacylglycerols in skeletal muscles and raise sensitivity to insulin in obese rats. Physical exercises raise the level of triacylglycerols in skeletal muscles in standard-diet rats that probably is the adaptation to high energy expenditure, but does not lead to high insulin resistance.
THE VALIDATION OF THE RESULTS OF MICROARRAY STUDIES OF ASSOCIATION BETWEEN GENE POLYMORPHISMS AND THE FREQUENCY OF RADIATION EXPOSURE MARKERS
Authors: Khalyuzova M. V. , Litvyakov N. V. , Sazonov A. E. , Albakh Ye. N. , Isubakova D. S. , Karpov A. B. , Takhauov R. M. 
Published: June 2020

The results from the selective validation research into the association between genetic polymorphisms and the frequency of cytogenetic abnormalities on a large independent sample are analyzed. These polymorphisms have been identified previously during own microarray studies. It has been shown an association with the frequency of dicentric and ring chromosomes induced by radiation exposure. The study was conducted among Siberian Group of Chemical Enterprises healthy employees (n = 573) exposed to professional irradiation in a dose range of 40–400 mSv. We have found that 5 SNP are confirmed to be associated with the frequency of dicentric and ring: INSR rs1051690 – insulin receptor gene; WRNrs2725349 – Werner syndrome gene, RecQ helicase-like; VCAM1 rs1041163 – vascular cell adhesion molecule 1 gene; PCTP rs2114443 – phosphatidylcholine transfer protein gene; TNKS rs7462102 – tankyrase gene; TRF1-interacting ankyrin-related ADP-ribose polymerase. IGF1 rs2373721 – insulin-like growth factor 1 gene has not confirmed to be associated with the frequency of dicentric and ring chromosomes.
FEATURES OF THE INTESTINAL MICROBIOTA IN PATIENTS WITH CHRONIC OBSTRUCTIVE PULMONARY DISEASE
Authors: Ogorodova L. M. , Govorun V. M. , Fedosenko S. V. , Karnaushkina M. A. , Saltykova I. V. , Alekseyev D. G. , Kostrytikova Ye. S. , Tyakht A. V. , Popenko A. S. 
Published: June 2020

The article summarizes the results of studies on the composition of microbial communities in stool samples of patients with chronic obstructive pulmonary disease (COPD) compared with healthy volunteers using genome-metagenomic sequencing. It is shown that the microbial community of the intestine in COPD patients is characterized as diverse taxonomic composition of metagenomes that the healthy volunteers microbiota. In this case, the normal composition of intestinal microbiota in patients with COPD is exposed to the qualitative and quantitative modifications. In contrast to healthy volunteers, the intestinal microbiota in patients with COPD is characterized by the presence of representatives of the Proteobacteria, as Citrobacter, Enterobacter, Eggerthella,Proteus, Salmonella, Anaerococcus, Clostridium difficile, Pseudomonas, as well as higher insemination fungus genusCandida (Candida dubliniensis and Candida albicans).
NEUROTROPIC AND DIURETIC ACTIVITY OF THICK EXTRACT OF THE BLOOD-RED HAWTHORN’S FRUITS
Authors: Kurkin V. A. , Kurkina A. V. , Zaitseva Ye. N. , Dubishchev A. V. , Pravdivtseva O. Ye. , Morozova T. V. 
Published: June 2020

Blood-red hawthorn (Crataegi fructus) are a popular cardiotonic herbal materials, which widely used in medical practice in our country and abroad. In order to prevent diseases of the cardiovascular system is appropriate to prescribe herbal medicines, because they combine the breadth and softness of therapeutic action, along with the absence of a significant number of side effects and contraindications.The purpose was to study diuretic and antidepressant activity of thick fruit extract of blood-red hawthorn.Material and methods. Under laboratory conditions we obtained a thick extract of blood-red hawthorn fruits. This phytopharmaceutical was studied by us for any diuretic and antidepressant activity. Investiga- tions were carried out on white rats of both sexes weighing 200–220 g. Each experimental group consist- ed of ten animals. Study drug was administered intragastrically by stomach tube. Control in both cases served as purified water. For determination of diuretic activity, we used a thick extract of hawthorn fruits in a dose of 20 mg/kg against the background of the water load in the amount of 3% of body weight of the animal. Reference preparation was chosen dichlorthiazid 20 mg/kg. It was administered once. After ad- ministration the animals were placed in metabolic cages for urine collection for 24 hours. The study de- termined diuresis, natriuresis, kaliuresis, and kreatininuresis for 4 h and 24 h of the experiment. On the day of the experiment the animals of the control group was administered water load of 3%, and experi- enced – intragastrically drug in an identical amount. Animals were placed in a metabolic cage for a day. Collected 4 h and 24-h urine sample. Determined by renal excretion of water was recorded concentration of sodium and potassium by flame photometry in the fiery liquid analyzer PAG-1, creatinine – colorimet- ric method for photocolorimeter CFC-3. For determining antidepressant activity we used a thick hawthorn extract in a dose of 25 mg/kg of animal body weight. As a synthetic drug comparisons, we used amitriptilin 5 mg/g.Results. It was found that the thick extract of fruits of blood red hawthorn in a dose of 20 mg/kg for 24 hours increases the diuresis experiment, both by increasing the glomerular filtration rate and by lowering tubular reabsorption. For thick extract of fruits of hawthorn blood-red characteristic expression antide- pressant activity.Conclusion. The studied preparation of blood-red hawthorn’s fruits have properties to provide complex nephrotropic and neurotropic activity on the living organism.
THE ROLE OF NEOPTERIN AS A POSSIBLE DIAGNOSTIC MARKER PREVALENCE EXTERNAL GENITAL ENDOMETRIOSIS
Authors: Novikova Ye. I. , Novikov D. G. , Indutny A. V. 
Published: June 2020

Objective. Todetermine the diagnostic value of neopterin as a marker of dysfunction of peritoneal immune factors for estimating the severity of external genital endometriosis (EGE).Material and methods. The concentration of neopterin in the peritoneal fluid (PF) and venous blood of 16 patients with EGE and 10 patients with benign ovarian cysts were evaluated.Results. There wasn’t significant relationship between the concentration of the neopterin in the venous blood and clinical data. There weren't significant relationship between the concentration of the neopterin in the venous blood and clinical data. There were the significant correlation between the concentration of neopterin in the PF of patients with EGE and pain by the B'n'B scale (r = 0.545, p = 0.044) and pain by NRC scale of (r = 0.362 p = 0.203). There were correlation between the concentration of neopterin in the PF of patients with EGE and the severity of endometriosis by rAFS scale (r = 0.598, p = 0.051), and between the neopterin concentration in PF and adhesions (r = 0.487, p = 0.128).Conclusion. Thus, it is possible to use the the neopterin concentrations in the PF of patients with EGE as a marker of disease severity and as a predictor of recurrence endometriosis.
Diagnostic et suivi de gestation chez la brebis : réalités et perspectives
Authors: EL AMIRI B. , KAREN A. , COGNIE Y. , SOUSA N.M. , HORNICK J.L. , SZENCI O. , BECKERS J.F. 
Published: June 2020

Cet article décrit les différentes méthodes cliniques et de laboratoire utilisables pour établir le diagnostic de la gestation chez les ovins. Etant destinées à des applications dans les élevages, ces méthodes sont présentées en insistant sur la précocité de leur utilisation, sur les paramètres de sensibilité, de spécificité, d’exactitude de diagnostic positif, d’exactitude de diagnostic négatif et enfin sur leurs possibilités de dénombrement des fœtus. La méthode la plus précoce est le dosage de la progestérone. Elle fournit dès le 17e jour des valeurs élevées d’exactitude mais nécessite de connaître exactement le moment de la fécondation. Le dosage des protéines spécifiques ou associées à la gestation (PSPB, PAG) est également une méthode précoce, le diagnostic étant obtenu dès le 22e jour pour un dosage dans le sang et le 32e jour pour un dosage dans le lait. Il présente d’excellentes performances de sensibilité et de spécificité, mais ne permet pas de dénombrer les fœtus. Enfin, l’échographie mode-B, utilisable dès le 30e jour de gestation, donne, selon l’opérateur, de très bonnes à d’excellentes performances de sensibilité et de spécificité et permet de dénombrer les fœtus en temps réel.
Connecting Science with Story Tale: How Sainsmatika Story Tale Book Decrease Science Anxiety of 4th Graders Student
Authors: Putri Agnestasia Ramadhani , Mustadi Ali 
Published: June 2020

This study investigating the effectiveness of using Sainsmatika story tale book towards student science anxiety. The study were an experimental research with pretest-posttest control-group design, involving 122 fourth-grade students in one of the district in Indonesia. Characteristically, this research employs quantitative data which were collected from the questionnaire results regarding science anxiety. The data were analyzed using statistical methods to calculate the values and determine the frequency as well as the mean of pretest and posttest scores in control and experimental classes. To determine the effectiveness of using fairytale books to reduce students’ science anxiety, the one-way analysis of ANOVA was used with a significant difference at the level of Sig < 0.05. The results of this study reveal that the use of sainsmatika-based fairytale books is effective to reduce science anxiety of the fourth-grade students with significancy 0.00 (<0.05).
Student Creativity through Project-based Learning Experiences
Authors: Desi Desi , Mujamil Jejem , Lesmini Bety , Hidayat Iceng 
Published: June 2020

This study aims to analyze students’ creativity through project-based learning experiences in a electrochemistry topic. Subject of this study was 40 students from Chemistry Education Study Program who took basic chemistry course. Students experienced several activities, namely: forming groups of three, asking and refining questions, debating ideas, making predictions, designing plans and/or experiments, collecting and analyzing data, drawing conclusions, communicating their ideas and findings to others, asking new questions, and creating artifacts like a model, a videotape, or a media. Then, the lecturer monitored the students and the progress of the project, assessed the outcome and evaluated the experience resulting students-in group’ creativity profile. Research instruments are project assessment rubrics and observation sheets. Findings show that students’ creativity in groups ranges from less to very good or from 48.15 to 92.59. Although there are still shortcomings with this study, we encourage other lecturers to implement this type of learning model in other courses or subjects in order to improve students’ problem solving skills.
EGG QUALITY OF SELECTED ACEH NATIVE CHICKENS FED ON THE DIETS COMPOSED OF Indigofera sp. MEAL BASED ON FEED FERMENTATION AS PARTLY SUBSTITUTION OF COMMERCIAL DIET
Authors: Yaman Muhammad Aman , Zulfan Zulfan 
Published: June 2020

The objective of this study was to determine the effect of inclusion of fermented feed consisted of 15% Indigofera sp. + 40% rice bran + 25% corn bran + 20% soybean meal as partial substitution of commercial diet on the egg quality of selected Aceh native (SAN) chickens. As many as 48 SAN pullets were used in this study. The study was performed in a completely randomized design, consisted of 4 treatments and 4 replications. Each replication was an experimental unit consisted of three chickens. The results of study indicated that inclusion of up to 15% fermented feed which was composed of 15% Indigofera sp. + 40% rice bran + 25% corn bran + 20% soybean meal as substitution of commercial layer diet significantly (P0.01) improved yolk index without adverse effect on albumen and eggshell quality of SAN chickens. As conclution, substitution of fermented feed contained 15% indigofera may improve the egg quality of SAN chickens.
THE EFFECT OF UREA SUPLEMENTATION IN MATURATION MEDIA OF BOVINE OOCYTE IN VITRO TOWARDS EXPRESSION OF BAX, BCL-2 AND BAX/BCL-2 RATIO
Authors: Kartikasari Dhesy , Mulyati Sri , Utama Suzanita , Srianto Pudji , Widjiati Widjiati , Plumeriastuti Hani 
Published: June 2020

This study aims to evaluate the expression of BAX, BCL-2, and BAX/BCL-2 ratio in maturation media of cow oocytes which supplemented with Urea in vitro because BAX and BCL-2 are the main regulators of apoptosis. A total of 263 oocytes from follicle aspirations originating from ovaries taken from slaughterhouses and were saturated with 3 addition of urea which was divided into three groups. The control group (P0) was control group without the addition of urea, P1 group was added with urea 20 mg/dL, while P2 group was added with urea 40 mg/dL. The results of in vitro oocyte maturation were continued with identification using immunocytochemical staining with the addition of BAX and BCL-2 antibodies. Positive results showed a brownish color on the oocyte and its cumulus. The results of this study indicated that there were significant differences (P0.05) in BAX and BCL-2 expression, although both curves were equally increase. The increase in BCL-2 was more significant than BAX, while the BAX BCL-2 ratio did not show a significant difference (P0.05) in whichthe curve of BAX/BCL-2 ratio was decreased. It can be concluded that the addition of urea does not affect the level of apoptosis.