bluuebunny/crossref_metadata_2025_split huggingface dataset:
On the way from SARS-CoV-sensitive mice to murine COVID-19 model
Authors: Soldatov Vladislav O. , Kubekina Marina V. , Silaeva Yuliya Yu. , Bruter Alexandra V. , Deykin Alexey V. 
Published: May 2020

The coronavirus disease 2019 (COVID-19) is a master killer which appeared suddenly and which has already claimed more than 200,000 human lives. In this situation, laboratories are in urgent need for a COVID-19 murine model to search for effective antiviral compounds. Here we propose a novel strategy for the development of mice that can be inoculated by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the COVID-19 causative agent. In humans, two proteins – ACE2 and TMPRSS2 – are involved in SARS-CoV-2 cells entry and, thus, we decided to intro­duce their genes into a murine genome. These genes will be placed with LoxP sites under the murine Tmprss2 promoter. Such an approach can provide a representative model with the opportunity to control the viral sensitivity of an animal population and tissue specificity of hACE2 and hTMPRSS2 expression.
Graphical abstract:
The new COVID-19 model should be based on inducible co-expression of the human ACE2 and TMPRSS2 genes. Activation of ACE2 and TMPRSS2 genes will occur only in the virological laboratory, after crossbreeding with Cre-mice. Before activation, mice will be resistant to SARS-CoV-2 for their biological safety during the pandemic.
A phytosociological survey of aquatic vegetation in the main freshwater lakes of Greece
Authors: Zervas Dimitrios , Tsiripidis Ioannis , Bergmeier Erwin , Tsiaoussi Vasiliki 
Published: May 2020

Aims: This study aims to contribute to the knowledge of European freshwater lake ecosystems with updated and new information on aquatic plant communities, by conducting national-scale phytosociological research of freshwater lake vegetation in Greece. Moreover, it investigates the relationship between aquatic plant communities and lake environmental parameters, including eutrophication levels and hydro-morphological conditions.Study area: Lakes in Greece, SE Europe.Methods: 5,690 phytosociological relevés of aquatic vegetation were sampled in 18 freshwater lake ecosystems during 2013–2016. The relevés were subjected to hierarchical cluster and indicator species analyses in order to identify associations and communities of aquatic vegetation, as well as to describe their syntaxonomy. Multiple regression analysis was applied to investigate the relationship between vegetation syntaxa and environmental parameters of lakes, i.e. physico-chemical parameters and water level fluctuation.Results: Ninety-nine plant taxa belonging to 30 different families were recorded. Forty-six vegetation types were identified and described by their ecological characteristics, diagnostic taxa and syntaxonomical status. Thirteen vegetation types, the largest number belonging to the vegetation classCharetea, are considered to be new records for Greece. The distribution of the vegetation types recorded in the 18 freshwater lakes was found to depend on environmental parameters and levels of eutrophication.Conclusions: An updated aquatic vegetation inventory was produced for Greek lakes, and primary results showed that the presence/absence of aquatic plant communities and the community composition in freshwater lakes can be utilized to assess the pressure of eutrophication on lake ecosystems.Taxonomic reference: Euro+Med (2006–).Abbreviations: MNT = Mean number of taxa; WFD = Water Framework Directive.
Le commerce à Bruxelles : réconcilier l’urbain avec un secteur en reconfiguration
Authors: Wayens Benjamin , Debroux Tatiana , Godart Pernelle , Mahieu Céline , Strale Mathieu , d'Ieteren Emmanuel 
Published: May 2020

La Région de Bruxelles-Capitale comptait en 2017 20 700 points de vente de biens et de services, par lesquels transitent 87 % des dépenses effectuées par les Bruxellois. Ce commerce de détail offre environ 9 % de l’emploi régional. Cette note de synthèse fait le point des connaissances sur la distribution à Bruxelles, un secteur économique indispensable, mais complexe à appréhender du fait de sa diversité et traversé par de profondes évolutions de la consommation, de l’environnement urbain et de l’organisation interne du métier. Après avoir esquissé le cadre de l’analyse en définissant le commerce au travers de ses fonctions, de ses formes et de ses acteurs, les auteurs dressent plusieurs constats associés à des dynamiques majeures du secteur (transformation de l’offre, de la demande et évolution de la régulation publique, notamment). La troisième partie de la note répond à ces constats par l’identification d’enjeux clairs dont la prise en compte par le secteur, soutenu par les autorités publiques, pourrait réconcilier le commerce et l’espace urbain.
GPA33: A Marker to Identify Stable Human Regulatory T Cells
Authors: Opstelten Rianne , de Kivit Sander , Slot Manon C. , van den Biggelaar Maartje , Iwaszkiewicz-Grześ Dorota , Gliwiński Mateusz , Scott Andrew M. , Blom Bianca , Trzonkowski Piotr , Borst Jannie , Cuadrado Eloy , Amsen Derk 
Published: May 2020

Abstract
FOXP3-expressing regulatory T (Treg) cells safeguard immunological tolerance. Treg cells can be generated during thymic development (called thymic Treg [tTreg] cells) or derived from mature conventional CD4+ T cells that underwent TGF-β–mediated conversion in the periphery (called peripheral Treg [pTreg] cells). Murine studies have shown that tTreg cells exhibit strong lineage fidelity, whereas pTreg cells can revert into conventional CD4+ T cells. Their stronger lineage commitment makes tTreg cells the safest cells to use in adoptive cell therapy, increasingly used to treat autoimmune and inflammatory disorders. Markers to distinguish human tTreg cells from pTreg cells have, however, not been found. Based on combined proteomic and transcriptomic approaches, we report that the Ig superfamily protein GPA33 is expressed on a subset of human Treg cells. GPA33 is acquired late during tTreg cell development but is not expressed on TGF-β–induced Treg cells. GPA33 identifies Treg cells in human blood that lack the ability to produce effector cytokines (IL-2, IFN-γ, IL-17), regardless of differentiation stage. GPA33high Treg cells universally express the transcription factor Helios that preferentially marks tTreg cells and can robustly and stably be expanded in vitro even without rapamycin. Expanded GPA33high Treg cells are suppressive, unable to produce proinflammatory cytokines, and exhibit the epigenetic modifications of the FOXP3 gene enhancer CNS2, necessary for indelible expression of this critical transcription factor. Our findings thus suggest that GPA33 identifies human tTreg cells and provide a strategy to isolate such cells for safer and more efficacious adoptive cell therapy.
Group 2 Innate Lymphoid Cells Promote Development of T Follicular Helper Cells and Initiate Allergic Sensitization to Peanuts
Authors: Krempski James W. , Kobayashi Takao , Iijima Koji , McKenzie Andrew N. , Kita Hirohito 
Published: May 2020

Abstract
Peanut allergy is a growing public concern; however, little is known about the immunological mechanism(s) that initiate the disease process. Our knowledge is also limited regarding the role of group 2 innate lymphoid cells (ILC2s) in regulating humoral immunity. To fill these major gaps in our knowledge, we investigated the immunological mechanisms involved in peanut allergen sensitization by using mouse models. To mimic environmental exposure in humans, naive BALB/c mice were exposed to peanut flour by inhalation without any exogenous adjuvants. When exposed to peanut flour, naive mice developed T follicular helper (Tfh) cells in their lung draining lymph nodes and produced IgE Abs to peanuts. Mice deficient in IL-13 showed decreased numbers of Tfh cells and germinal center B cells and produced significantly fewer IgE Abs. IL-13 was necessary and sufficient for induction of CD11c+ MHC class IIhi dendritic cells that are implicated in Tfh cell development. Importantly, lung ILC2s served as a predominant early source of IL-13 when naive mice were exposed to peanut flour. Furthermore, mice that are deficient in lung ILC2s by bone marrow transfer from Rorasg/sg mice or by genetic manipulation produced significantly fewer IgE Abs to peanuts compared with control mice. These findings suggest lung ILC2s that serve as a rapid source of IL-13 upon allergen exposure play a major role in Tfh cell development, IgE Ab production, and initiation of peanut allergy.
ANÁLISE DE VIABILIDADE ECONÔMICA E FINANCEIRA DE IMPLANTAÇÃO DE UMA CERVEJARIA ARTESANAL EM RECIFE/PE
Authors: Rapôso Cláudio Filipe Lima , Matsumoto André Rodrigues , Brito Giovanni Bruno Lopes de Souza , De Lima Haniel Melo 
Published: May 2020

O desenvolvimento e planejamento de novos negócios são ações cruciais para o sucesso em tempo de diversas integrações internacionais, pois, as pessoas estão cada vez mais preocupadas e exigentes com a forma, a qualidade, e o custo em contraste ao valor agregado do produto final. É no desenvolvimento desses planos que se evidencia ações questionáveis e que possam trazer prejuízos à empresa, à sua posição e criticidade em relação a fatores internos e externos, e externar possíveis diferenciais entre os concorrentes e a capacidade de corrigir possíveis problemas. Este trabalho tem como hipótese de que o mercado em Recife-PE ainda é lucrativo e dessa forma apresenta como objetivos específicos: Calcular a receita e despesa da implantação de um microcervejaria artesanal na região, calcula e analisar os indicadores de viabilidade.
Referências
DORNELAS, J. C. A. Empreendorismo: transformando idéias em negócios. Terceira.
Rio de Janeiro: Elsevier, 2008.
FAUSTINO, A. Z. Planejamento do fluxo de caixa em microempresas — Universidade Federal de Santa Catarina, Florianópolis, 2005.
IBGE. Tipos de comércio. 2012. Acesso em 06/04/2016. Disponível em:<http://www.ibge.gov.br>.
IBGE. IBGE divulga as Estimativas de População dos municípios para 2018. 2018. Disponível em: <https://agenciadenoticias.ibge.gov.br/agencia-sala-deimprensa/2013-agencia-de-noticias/releases/22374-ibge-divulga-as-estimativas-depopulacao-dos-municipios-para-2018>. Acesso em: 05/03/2019.
IBGE. Cidades IBGE. 2019. Disponível em: <https://cidades.ibge.gov.br/brasil/sc/ timbo/panorama>. Acesso em: 16/06/2019.
OLIVEIRA, A. D. de. Planejamento Financeiro em uma microempresa — Universidade Federal do Rio Grande do Sul, Porto Alegre, 2010. Acesso em 22/05/2016. Disponível em: <http://www.lume.ufrgs.br/bitstream/handle/10183/29611/000768588.pdf>.
Revista Forbes Brasil. 10 países que mais beberam e compraram cerveja no mundo em 2017. 2018. Artigo. Disponível em: <https://forbes.com.br/listas/2018/01/10paises-que-mais-bebem-e-compram-cerveja/>. Acesso em: 27/11/2019.
SILVA, A. D. da. Análise das demonstrações financeiras da Intelbrás — Universidade Vale do Itajaí, São José, 2005. Acesso em 22/05/2016. Disponível em: <http:
//siaibib01.univali.br/pdf/AdilsonSilva.pdf>.
ZDANOWICZ, J. E. Fluxo de caixa: uma decisão de planejamento e controle financeiro. Decima. Porto Alegre: Sagra Luzzatto, 2004.
Reflexiones para una práctica docente de integración de destrezas y contenidos en los programas de español a nivel universitario
Authors: Soriano Salkjelsvik Kari 
Published: May 2020

SUMMARY This article is a reflection on the possibilities that the concept of Content and Language Integrated Learning (CLIL), can provide for the teaching of foreign language programs at the university level, using as an example the programs of Hispanic studies in Norway. After reviewing the concept of CLIL, and placing it in the context of Hispanic studies, the article investigates its practical, political, and educational applications. It is argued that integrating skills and content fully at the university level is a task that depends on specific administrative infrastructures, as well as on methodological resources that can make this integration an object of study and reflection. In this context, this work aims to promote the creation of new teaching proposals for university programs of foreign languages ​​that respond to the challenges and demands that are currently facing the humanities. RESUMEN Este artículo se concibe como una reflexión sobre las posibilidades que el concepto de Aprendizaje integrado de contenido y lengua (AICLE), puede aportar para la enseñanza los programas de lenguas extranjeras en las universidades, utilizando como ejemplo los programas de estudios hispánicos en Noruega. Tras revisar el concepto de CLIL, y situarlo en el contexto de los estudios hispánicos, el artículo indaga sobre su aplicación práctica y político-educativa. Se arguye que integrar destrezas y contenidos plenamente a nivel universitario es una tarea que depende de infraestructuras administrativas específicas, así como de recursos metodológicos que hagan de dicha integración un objeto de estudio y reflexión. En este sentido, este trabajo quiere impulsar la creación de nuevas propuestas docentes para los programas universitarios de lenguas extranjeras que respondan a los retos y exigencias a los que actualmente se están enfrentando las humanidades.
Todo mundo pode escolher o feminismo: o convite de Bell Hooks
Authors: Franco Luciele Mariel 
Published: May 2020

HOOKS, bell. O feminismo é para todo mundo: políticas arrebatadoras. 1 ed. Rio de Janeiro: Rosa dos Tempos, 2018. E-book.O Feminismo é para todo mundo: políticas arrebatadoras (2018) pauta-se na compreensão que bell hooks tem de feminismo para analisar os problemas enfrentados pelo movimento, elencar as conquistas já obtidas e apontar as pautas a serem desenvolvidas, tendo em vista as estruturas opressoras ainda vigentes. A autora defende que o feminismo deve ser fruto de uma escolha consciente e, para isso, é necessário que uma educação feminista seja difundida em massa.
The Typesetter of James McTeigue's The Raven: Poe's Dual Fandom in Post-Millennial Transmedia Culture
Authors: Herrera José Manuel Rodríguez 
Published: May 2020

Abstract
Over the first two decades of the twenty-first century, Edgar Allan Poe's characters and persona have thrived in ever mutating incarnations appealing to wider audiences around the world. Some of these Poe-inspired mash-up incarnations are instilling in the public new doses of enthusiasm and fervor for everything related to Poe's work and persona. In our post-millennial transmedia culture Poe fans are actively engaged with refashioning and adapting everything they adore about Poe's work and life. Thus, Poe fandom plays a pivotal role in disseminating new Poe-inspired adaptions across a wide array of media platforms. Thanks to what Henry Jenkins calls “participatory culture,” the post-millennial Poe has reached the status of an “industry unto itself.” After all, participatory culture, Jenkins concludes, “is anything but fringe or underground today.” However, as I try to argue, Poe's ubiquitous presence in post-millennial popular culture has been boosted by a new phenomenon. I'm referring to the merging of modernized traits of two of the most popular characters ever to emerge from the pages of the mystery genre: C. Auguste Dupin and Sherlock Holmes. The upshot of this is the convergence of the Poe fandom and the Sherlock fandom, two of the most active fandoms in transmedia culture. James McTeigue's 2012 film The Raven tries to pander to the tastes of this dual fandom by capitalizing on the success of the BBC series Sherlock and Guy Ritchie's Sherlock movie adaptations, Sherlock Holmes (2009) and Sherlock Holmes: A Game of Shadows (2011).
VIH-Sida: La epidemia olvidada en Honduras
Authors: Retes Eduardo 
Published: May 2020

Ante la emergencia el día 1 de diciembre, Día Mundial del Sida, pasó sin pena ni gloria. Revisando literatura alusiva llama la atención la similitud del acontecer en Honduras con el artículo “La prevención olvidada: reemergencia del VIH en Chile” (Stuardo, 2017).
Amelioration of Drought Stress on Plants under Biostimulant Sources
Authors: Carolina Feitosa de Vasconcelos Ana 
Published: May 2020

Water stress is one of the most important environmental factors inducing physiological changes in plants, such as decreasing water potential of the cells and the stomatal closure, resulting in reduced CO2 availability for the plants and inhibiting photosynthesis. One common feature of these stress conditions is the development of oxidative processes mediated by reactive oxygen species (ROS). ROS accumulate in the cells and cause damage in important cellular components, such as thylakoids and chloroplasts. Plants have antioxidant defense systems to cope with ROS. Antioxidants enzymes superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX) are efficient scavengers of ROS: superoxide, hydroxyl radicals, and singlet oxygen. The activities of antioxidant enzymes in plants are normally favored when plants are subjected to some kind of improvement in the conditions in which they are grown. In this sense, biostimulants cause changes in vital and structural processes in order to influence plant growth through improved tolerance to abiotic stresses by increasing the antioxidant activity in plants.
Fauna associated with shallow-water methane seeps in the Laptev Sea
Authors: Vedenin Andrey A. , Kokarev Valentin N. , Chikina Margarita V. , Basin Alexander B. , Galkin Sergey V. , Gebruk Andrey V. 
Published: May 2020

BackgroundMethane seeps support unique benthic ecosystems in the deep sea existing due to chemosynthetic organic matter. In contrast, in shallow waters there is little or no effect of methane seeps on macrofauna. In the present study we focused on the recently described methane discharge area at the northern Laptev Sea shelf. The aim of this work was to describe the shallow-water methane seep macrofauna and to understand whether there are differences in macrobenthic community structure between the methane seep and background areas.MethodsSamples of macrofauna were taken during three expeditions of RVAkademik Mstislav Keldyshin 2015, 2017 and 2018 using 0.1 m2grabs and the Sigsbee trawl. 21 grabs and two trawls in total were taken at two methane seep sites namedOdenandC15, located at depths of 60–70 m. For control, three 0.1 m2grabs were taken in area without methane seepage.ResultsThe abundance of macrofauna was higher at methane seep stations compared to non-seep sites. Cluster analysis revealed five station groups corresponding to control area,Odensite andC15site (the latter represented by three groups). Taxa responsible for differences among the station groups were mostly widespread Arctic species that were more abundant in samples from methane seep sites. However, high densities of symbiotrophic siboglinidsOligobrachiasp. were found exclusively at methane seep stations. In addition, several species possibly new to science were found at several methane seep stations, including the gastropodFrigidalvaniasp. and the polychaeteOphryotrochasp. The fauna at control stations was represented only by well-known and widespread Arctic taxa. Higher habitat heterogeneity of theC15site compared toOdenwas indicated by the higher number of station groups revealed by cluster analysis and higher species richness inC15trawl sample. The development of the described communities at the shallow-water methane seeps can be related to pronounced oligotrophic environment on the northern Siberian shelf.
On the importance of spatial scales on beta diversity of coral assemblages: a study from Venezuelan coral reefs
Authors: Miyazawa Emy , Montilla Luis M. , Agudo-Adriani Esteban Alejandro , Ascanio Alfredo , Mariño-Briceño Gloria , Croquer Aldo 
Published: May 2020

Estimating variability across spatial scales has been a major issue in ecology because the description of patterns in space is extremely valuable to propose specific hypotheses to unveil key processes behind these patterns. This paper aims to estimate the variability of the coral assemblage structure at different spatial scales in order to determine which scales explain the largest variability on β-diversity. For this, a fully-nested design including a series of hierarchical-random factors encompassing three spatial scales: (1) regions, (2) localities and (3) reefs sites across the Venezuelan territory. The variability among spatial scales was tested with a permutation-based analysis of variance (Permanova) based on Bray-Curtis index. Dispersion in species presence/absence across scales (i.e., β-diversity) was tested with a PermDisp analysis based on Jaccard’s index. We found the highest variability in the coral assemblage structure between sites within localities (Pseudo-F = 5.34; p-value = 0.001, CV = 35.10%). We also found that longitude (Canonical corr = 0.867, p = 0.001) is a better predictor of the coral assemblage structure in Venezuela, than latitude (Canonical corr = 0.552, p = 0.021). Largest changes in β-diversity of corals occurred within sites (F = 2.764, df1= 35, df2 = 107, p = 0.045) and within localities (F = 4.438, df1= 6, df2 = 29, p = 0.026). Our results suggest that processes operating at spatial scales of hundreds of meters and hundreds of kilometers might both be critical to shape coral assemblage structure in Venezuela, whereas smaller scales (i.e., hundreds of meters) showed to be highly- important for the species turnover component of β-diversity. This result highlights the importance of creating scale-adapted management actions in Venezuela and likely across the Caribbean region.
Association between dietary mineral nutrient intake, body mass index, and waist circumference in U.S. adults using quantile regression analysis NHANES 2007–2014
Authors: Jiang Shan , Ma Xiaoyu , Li Meng , Yan Shoumeng , Zhao Hantong , Pan Yingan , Wang Changcong , Yao Yan , Jin Lina , Li Bo 
Published: May 2020

Objective
Mineral nutrients play an important role in maintaining material and energy metabolism. Reports on mineral nutrient intakes and body mass index (BMI) and waist circumference (WC) are rare in the United States. This study examined the relationship between BMI, WC and dietary mineral intakes.

Method
We used the data from National Health and Nutrition Examination Survey 2007–2014. Nutrient intakes were adjusted for energy according to the residual adjustment method. We used the quantile regression model to analyze the relationship between BMI, WC under different distributions and the average daily mineral intakes.

Result
A total of 19,952 people were included in the study, including 9,879 men and 10,073 women (≥20 years old). The median BMI was 27.935 kg/m2 and the median WC was 97.700 cm. The results of quantile regression showed that calcium, magnesium, potassium, copper, zinc and iron intakes were negatively correlated with BMI and WC, after adjusting for age and gender. Sodium and phosphorus intakes were positively correlated with BMI, sodium intakes were positively correlated with WC. This correlation was enhanced with increasing quantiles of risk levels. In high BMI or high WC populations, mineral intakes had a greater impact on BMI and WC. The quantile regression coefficients of selenium intakes were not statistically significant at each quantile.

Conclusion
Our results suggested that the mineral nutrient intakes were associated with BMI and WC in American adults. However, we also need to further study the longitudinal effects of mineral intakes and obesity.