bluuebunny/crossref_metadata_2025_split huggingface dataset:
Agrowisata Berkelanjutan Berbasis Kopi di Satria Agrowisata Desa Manukaya, Kecamatana Tampak Siring, Kabupaten Gianyar, Bali (Studi Evolusi Pariwisata)
Authors: Azizah Febrytha Nur , Anom I Putu 
Published: December 2020

Agro-tourism is an alternative tourism activity that relies on plantations and agriculture as its main attraction. Along with the development of tourism, agro-tourism has now become an economic driving commodity for the surrounding community, so that agro-tourism is increasingly taken into account in the world of tourism. The development of an agro-tourism can not be separated from the evolutionary process that occurs through various stages of the beginning of the tourist attraction built until now. This study aims to determine the evolution of developments in Satria Agrowisata. The research method used is descriptive qualitative by conducting data collection techniques through online interview as primary data, and conducting online observations as secondary data. The results show that Satria Agrowisata can adapt well to the various changes that exist and continue to innovate in order to survive in the world of tourism until now. In Darwin's theory of evolution, he put forward two key words in his theory, natural selection and adaptation. Natural selection as a mechanism for evolutionary change, and adaptations that occur in its development over time.

Keyword: Evolution, Agrotourism, Satria Agrowisata, Bali.
Persepsi Pengunjung Domestik Terhadap Wisata Entertainment di Daya Tarik Wisata Kawasan Luar Pura Uluwatu
Authors: Tjampan Kesya Marcella , Nugroho Saptono 
Published: December 2020

The strategy forming tourist attraction to attract repeating guest is by developing entertainment tourism which offers sense of pleasure, satisfaction, and valuable experience. Uluwatu Kecak Fire Dance in Outer Area Uluwatu Temple was recognized as the most famous entertainment forms by domestic visitors. This aim of study is to identifying characteristics and measuring perception of domestic visitors to Uluwatu Kecak Fire Dance based on seven operational dimensions of entertainment tourism (Learning, Enjoyment, Escape, Refreshment, Novelty, Involvement, and Local Culture). The research used qualitative method with data collection techniques are questionnaire (number of samples were 65 respondents) and literature study. The data analysis technique used descriptive statistical analysis with continuum lines. The results showed that the perception of 65 domestic visitors for entertainment tourism in Uluwatu Temple were strongly agreed on Learning (277.5) and Enjoyment (285). While perception that were agreed are Escape (227), Refreshment (248.5), Novelty (259.25), Involvement (266), and Local Culture (272.33). In determining the dominant indicators of each dimension of entertainment tourism, the author uses the highest frequency capacity value. The three dimensions with highest average value are Enjoyment (285), Learning (277.5), and Local Culture (272.33). The administrator Outer Area Uluwatu Temple emphasize strategies that can maintain the three dimensions of entertainment tourism that have a dominant value, which is the Enjoyment dimension, Learning dimension, and the Local Culture dimension.

Keyword: Visitor Perception, Socio-demographic Characteristics, Entertainment Tourism, Domestic Visitors
Alternative viewpoints on the nature and importance of a prominent syncline at the northeastern edge of Wyoming’s Hanna Basin
Authors: Lillegraven Jason A. 
Published: December 2020

ABSTRACTThe geologic history of Wyoming’s Hanna Basin is still being written. Surprisingly, here appeared an opportunity to share insights from previously accomplished work with that conducted anew by other scholars. The area of study was in the southeastern quadrant of Wyoming, which exhibits the state’s most complex history with respect to the Laramide orogeny. Especially important for present purposes were the tectonic conditions of the late Paleocene and earliest Eocene, recorded within the Hanna Formation. Of central focus is the 2020 publication by Dechesne and her six co-authors. Geographically, the landscape they covered was a thin, synclinal slice of the northeastern margin of the Hanna Basin. Key goals for the present publication have been to illustrate positive linkages and to highlight discrepancies between Dechesne et al. (2020) and relevant prior geological work. A concern that permeates all facets of this approach is the ability to verify viability of brand-new geologic descriptions, data, and resulting conclusions. Essential graphical elements were introduced first into this present publication. Once that package of background information was available, more focused analyses were rigorously pursued on diverse issues within the Dechesne et al. (2020) publication. Dechesne’s team presented a significantly modified but adequately defended approximation of the Paleocene–Eocene boundary. Data from fossil plants (macro- and palynofloras), continental mollusks, and bulk organic-carbon isotopes all agree within one measured section (of five sections studied) with an approximated Paleocene–Eocene boundary along with a ‘carbon isotope excursion’ (CIE). Strength of available evidence seems questionable, however, in that the inordinately high variability in bulk organic carbon (characteristic of a CIE) has been demonstrated only in the Hanna Draw Section. Although fluvial, paludal, and lacustrine facies are considered in several contexts, in no sense does the publication’s organizational form provide a ‘detailed stratigraphic framework.’ One zircon-based U–Pb depositional date (54.42 ± 0.27 Ma) came from this study that matched early Wasatchian time. Participants in the Dechesne et al. (2020) project are to be commended in that their resulting paper ranged broadly across the geologic setting, stratigraphy, paleocurrents, paleobotany, continental mollusks, zircon geochronology, associated lithofacies, and paleogeography. Despite that breadth, there exists a plethora of unexpected and wholly avoidable inconsistencies, strong contradictions within what should be homogeneous datasets, and seemingly inexplicable omissions of obviously necessary and sometimes clearly existing but unutilized data, one must question the reliability of much of the information presented in their paper.
Transient Brugada-like ECG pattern incited by uncontrolled hypertension - a rare case report
Authors: Parajuli Priyanka , Bhattarai Mukul , Lara-Garcia Odalys Estefania , Regmi Manjari Rani , Maini Ruby , Sambandam Alarmelu , Kulkarni Abhishek 
Published: December 2020

Background:
Brugada syndrome (BrS) is a rare form of channelopathy caused by mutation of SCN5A gene that encodes for sodium channel and is associated with increased risk of ventricular arrhythmia and sudden cardiac death. BrS has a characteristic electrocardiographic (ECG) pattern. Various stressors can induce Brugada-like patern on ECG in the absence of BrS known as Brugada phenotype.

Case Presentation:
We report a case of a 28-year-old African-American male patient who presented to the emergency department with a chief complaint of atypical chest pain. He was noted to have an elevated blood pressure and a type 1 Brugada patern on his 12-lead ECG. His ECG changed from type 1 to type 3 Brugada patern upon controlling blood pressure, which indicated the presence of a Brugada phenocopy.

Conclusion:
It is important to recognize the underlying stressors that can lead to Brugada-like patterns on the ECG since these stressors are treatable without any sequel.
LYSOSOMAL STORAGE DISEASES: KIRIKKALE UNIVERSITY EXPERIENCE
Authors: BÜLBÜL Selda , ÇELİK Cansu , ALPCAN Ayşegül 
Published: December 2020

Objective: Lysosomal storage diseases which were first described in 1880; are important group of metabolic disorders characterized by the deposition of the substrates in lysosomes due to defects of the activity or transport of lysosomal enzymes or a defect in the receptor proteins. LSDs usually show a progressive clinical course and may not be represented with any clinical signs during the neonatal period. The overall prevalence of LSDs is 1 / 7000-8000. The aim of this study was to share the clinical characteristics of our LSDs patients and the experiences of our pediatric metabolic diseases department.Material and Methods: This retrospective cohort study was conducted at Kırıkkale University Hospital with 56 patients diagnosed as lysosomal storage disease among 315 patients diagnosed with metabolic diseases. Data were collected from outpatient clinic patient files who were diagnosed between 2011- 2018. Results: A total of 315 patients diagnosed with inherited metabolic disease were followed in our clinic and 56 (17.7 %) of them were diagnosed as LSDs. The 56 patients were suffering from the following diseases: 10 patients with Mucopolysaccharidosis, 1 patient with mucolipidosis type 2 (I-cell disease), 41 patients with sphingolipidoses, two patients with cystinosis, one patient with Infantile Pompe Disease and one patient with beta-mannosidosis.The mean age of the patients with Fabry Disease and the other patients diagnosed with other LSDs were 34.7±14.2 years (minimum 8, maximum 64) and 2.67±3.4 years (minimum 0, maximum 10.5) respectively. All diagnoses were verified by specific enzyme analysis and/or by conducting genetic mutation analysis. Conclusion: The most common lysosomal storage disease among our patients were Mucopolysaccharidosis and sphingolipidosis. Treatment options, such as enzyme replacement therapy and bone marrow transplantation exist, and 24 of these patients are receiving enzyme replacement therapy.
الأثار القانونية لتغير سيادة الدولة على الحقوق المكتسبة للأفراد الأجانب
Authors: Ahmad Bin Issa 
Published: December 2020

لما كانت الحقوق المكتسبة للأفراد الأجانب تتأثر بتغير السيادة على الدول. لذا أقرت المواثيق الدولية إلزام إعمال المبادئ العامة للقانون الدولي خاصة ما تعلق بالممتلكات لأنها حقوق مكتسبة للأفراد الأجانب في ظل سيادة دولة سابقة قبل أن تحل محلها سيادة دولة أخرى.
إلا أن معظم الدول لم تلتزم بذلك وقامت بإستعادة وتأميم الممتلكات الأجنبية وهذا ما كان في حالة الجزائر كدولة مستقلة.
Ticagrelor alleviates sepsis-induced myocardial injury via an adenosine-dependent pathway in a mouse sepsis model
Authors: Tang Shengxing , Fu Cong , Xu Qiancheng , Guo Wenjun , Cao Yuhan 
Published: December 2020

Purpose: The purpose of this study was to determine whether ticagrelor, a classic anti-platelet drug, has a therapeutic effect on sepsis-induced myocardial injury.

Methods: The C57BL6J mice received oral ticagrelor (10, 25 and 50 mg/kg) for seven days after which cecum ligation and puncture (CLP) were performed. An adenosine-receptor antagonist (CGS15943) was administered two hours before CLP. After 24 h, cardiac function was measured using cardiac echocardiography, then the heart and blood were collected. Hematoxylin and eosin (HE) staining and terminal deoxynucleotidyl transferase
dUTP nick end labelling (TUNEL staining) were used to observe pathological changes and cardiomyocyte apoptosis. Plasma concentration of TNF-α, IL-6 and adenosine and myocardial tissue levels of TNF-α and IL-6 were determined. Survival analysis was performed. Western blot was used to determine the expression of a signalling protein in the myocardial tissue.

Results: The HE and TUNEL staining showed less inflammatory cell infiltration and less cardiomyocyte apoptosis in the ticagrelor group. Cardiac echocardiography showed preserved heart function in the ticagrelor group. Plasma TNF-α, IL-6 and relative expression of TNF-α and IL-6 in myocardial tissue were significantly lower in the ticagrelor group. Plasma adenosine levels were significantly higher in the ticagrelor group. Adenosine-receptor antagonists significantly blocked the protective effect of ticagrelor. Ticagrelor reduced the mortality of sepsis mice, and this reduction was blocked by the adenosine-receptor antagonist. Western blot showed that ticagrelor activated the phosphorylation of AKT and mTOR. Adenosine-receptor antagonists inhibited the activation of AKT and mTOR.

Conclusion: The protective effect of ticagrelor was dependent on adenosine-receptor activation, with downstream upregulation of phosphorylation of AKT and mTOR.
Colloid cyst curtailed: A case report of spontaneous colloid cyst regression
Authors: Cosgrove Megan E. , Saadon Jordan , Chesler David A. 
Published: December 2020

Background:
Colloid cysts arise from the roof of the third ventricle and are at risk for obstructing the flow of cerebrospinal fluid (CSF) and causing increased intracranial pressure. With advancements and increased frequency of imaging, colloid cysts are sometimes discovered incidentally. In these cases, the neurosurgeon is faced with the decision of whether to intervene or manage conservatively.

Case Description:
A 67-year-old man was discovered to have a colloid cyst when imaging was performed for transient neurologic deficits. CT and MRI brain revealed a 5mm lesion in the third ventricle with characteristics suggestive of the colloid cyst. Except for his initial presentation, the patient did not exhibit any symptoms and was followed with serial imaging. Four years after discovery, the colloid cyst regressed in size.

Conclusion:
The evolution and resolution of colloid cysts remain elusive; however, the discovery of incidental colloid cysts due to more frequent and more advanced neuroimaging emphasize the importance of this topic. The fear of conservative management is acute decompensation due to obstruction of CSF. However, surgical risks may be avoided if these asymptomatic lesions regress and resolve without intervention. Conservative management is a viable option for patients with colloid cysts, who may not only avoid surgery but who might also rarely experience cyst resolution.
Experimental assessment of major regulatory systems (adrenal, thyroid and gonadal) in liver metastases from s45 sarcoma
Authors: Каплиева И.В. , Франциянц Е.М. , Трепитаки Л.К. , Черярина Н.Д. 
Published: December 2020

Метастазирование в печень - частый признак прогрессирования злокачественного процесса, механизм которого до конца не изучен. Цель - изучение особенностей функционирования основных систем нейрогуморальной регуляции: надпочечниковой (ГГНС), тиреоидной (ГГТС) и гонадной (ГГГС), на этапах метастазирования саркомы 45 (С45) в печень. Методика. Работа выполнена на 43 белых крысах-самцах. Через 1, 2, 5, 7 нед. от момента введения клеток саркомы 45 в дислоцированную под кожу селезенку. Органы взвешивались, в сыворотке крови (СК) методом РИА исследовали уровни фолликулостимулирующего гормона, лютеинизирующего гормона (ЛГ), тиреотропного гормона, адренокортикотропного гормона, кортизола, альдостерона, общей формы тестостерона, свободной и общей форм тироксина и трийодтиронина (Тсв и Т); методом ИФА - эстрона, эстрадиола, свободного тестостерона. Результаты. Метастазирование в печень сопровождалось активацией с последующим истощением ГГНС со снижением в 1,8 раза уровней кортизола и альдостерона в крови; значительной активацией ГГГС (пятикратное увеличение ЛГ в крови и уменьшение в 1,7 раза массы семенников) вследствие гипотестостеронемии (в 9,7 раза) на фоне гиперэстрадиолемии (в 2,3 раза); активацией ГГТС с формированием «low T» синдрома (в 4,6 раза). Заключение. Процесс метастазирования в печень - системная патология, в основе которой лежат глубокие нарушения работы основных систем регуляции организма.
Aim: To evaluate functioning of three major systems of neurohumoral regulation, adrenal (HPA), thyroid (HPT), and gonadal (HPG) axes, in liver metastasis based on weights of organs and blood levels of hormones. Methods: The study included 34 white male rats. Organs were weighted at 1, 2, 5, and 7 weeks after S45 sarcoma cell injections into the subcutaneously transferred spleen; blood serum levels of FSH, LH, TSH, ACTH, cortisol, aldosterone, total testosterone, free and total thyroxine, and triiodothyronine were measured by RIA; estrone, estradiol, and free testosterone concentrations were measured by ELISA. Results. The process of liver metastasis was accompanied by activation and following exhaustion of the HPA axis with 1.8 time decreases in blood levels of cortisol and aldosterone , significant activation of the HPG axis (5-fold increased LH level and 59% decreased testicular weight) due to hypotestosteronemia (9.7 times) and hyperestradiolemia (2.3 times), and activation of the HPT axis with the low T3 syndrome (4.6 times). Conclusion. Liver metastasis is a systemic pathology based on profound dysfunction of the major regulatory systems.
Detection of intracellular silver nanoparticles using flow cytometry
Authors: Каба С.И. , Соколовская А.А. 
Published: December 2020

Продемонстрировано обнаружение наночастиц серебра во внутриклеточном пространстве с помощью проточной цитофлуориметрии. В эндотелиальных клетках линии EA.hy926, инкубированных в растворе, содержащем 2 мкг/мл наносеребра, измеряли боковое светорассеяние. По сравнению с контрольными образцами этот параметр возрастал, в то время как прочие значимые характеристики не изменялись. Это подтверждает чувствительность метода к изменившемуся состоянию клеток и указывает на поглощение наночастиц серебра клетками при концентрации ниже токсической.
The study demonstrated a possibility for detection of intracellular silver nanoparticles using flow cytometry. The parameter used in this work, side scattering, was measured in EA.hy926 endothelial cells incubated in a 2 mg/ml silver nanoparticle solution. This parameter was increased compared to control samples. Therefore, this technique was sensitive to changes in the cell status and suggested the cell uptake of the particles under the subtoxic conditions.
Programming the anti-tumor immune response in vitro and its application to stop the growth of tumor cells and prolonging the lifespan of mice with carcinoma in vivo
Authors: Калиш С.В. , Лямина С.В. , Раецкая А.А. , Буданова О.П. , Малышев И.Ю. 
Published: December 2020

Цель - представить доказательства правомерности гипотезы, что комбинированный пул репрограммированных in vitro макрофагов и лимфоцитов будет эффективно ограничивать пролиферацию опухолевых клеток in vitro , а при введении в организм будет существенно ограничивать развитие опухоли in vivo . Методика. Размножение опухолевых клеток инициировали in vitro путем добавления клеток карциномы Эрлиха (КЭ) в среду культивирования RPMI-1640. Развитие асцитной опухоли in vivo воспроизводили путем внутрибрюшной инъекции клеток КЭ мышам. Результаты. Установлено, что M3 макрофаги вместе с антиген-репрограммированными лимфоцитами оказывают выраженный противоопухолевый эффект и in vitro, и in vivo , который был существеннее противоопухолевого эффекта цисплатина. Заключение. Факты, свидетельствующие, что М3 макрофаги в сочетании с in vitro антиген-репрограммированными лимфоцитами значительно подавляют рост опухоли in vivo , делают перспективным разработку клинической версии биотехнологии ограничения роста опухоли путем предварительного программирования противоопухолевого иммунного ответа «в пробирке».
Aim. To test a hypothesis that a combined pool of in vitro reprogrammed macrophages and lymphocytes will effectively limit growth of tumor cells in vitro , and injections of these cells into the body will considerably limit development of a tumor in vivo . Methods. Tumor growth was initiated in vitro by addition of Ehrlich carcinoma (EC) cells to the RPMI-1640 cell culture medium and in vivo by intraperitoneal injection of EC cells into mice. Results. M3 macrophages in combination with antigen-reprogrammed lymphocytes exerted a pronounced antitumor effect both in vitro and in vivo, which was superior to the effect of cisplatin. Conclusion. M3 macrophages in combination with in vitro antigen-reprogrammed lymphocytes significantly inhibited the tumor growth in vivo . This fact justifies development of a clinical version of the tumor growth restricting biotechnology using pre-programming of the antitumor immune response in vitro .
Neurogenesis in adult human brain. Substantiation of a therapeutic approach
Authors: Гомазков О.А. 
Published: December 2020

Нейроны взрослого мозга, формирующиеся в процессе нейрогенеза, являются непременными участниками важных физиологических процессов. Новые клетки играют важную роль в поддержке когнитивных функций, обучения и памяти. Эндогенная активация нейральных прогениторных клеток отмечена в ответ на патологические изменения - ишемия, нейродегенеративные заболевания, физическая травма, психические расстройства. Однако на фоне большого числа исследований, выполняемых в эксперименте, понимание роли новых клеток в мозге человека остается предметом больших сомнений. Задача данной статьи - проанализировав информацию о нейрогенезе в мозге ЧЕЛОВЕКА, обозначить звенья эндогенной регуляции и обрисовать возможные «мишени» репаративной терапии.
Neurons of the adult brain formed in the course of neurogenesis are indispensable participants of important physiological processes. New cells play an important role in supporting cognitive functions, learning and memory. Endogenous activation of neural progenitor cells has been noted to respond to pathological changes, such as ischemia, neurodegenerative diseases, physical trauma, and mental disorders. However, despite a large number of experimental studies, understanding of the role of new cells in human brain remains a matter of great doubt. This review focuses on analyzing information about neurogenesis in the human brain, identifying steps of endogenous regulation, and outlining possible «targets» of reparative therapy.
СОВРЕМЕННЫЕ ТЕХНОЛОГИИ – РАДИКАЛЬНЫЙ ПУТЬ ОХРАНЫ ОКРУЖАЮЩЕЙ СРЕДЫ
Authors: Харченко Иван Семенович , Харченко Лана Ивановна , Иванова Дарья Евгеньевна , Иванов Вячеслав Евгеньевич 
Published: December 2020

Экологические проблемы сложны и многообразны, отличаются широтой и неопределенностью, носят всеобъемлющий, глобальный характер. В настоящее время практически нет страны, где бы вопросы взаимодействия в системе «человек – природа – общество» не стояли столь остро и актуально. Сегодня на земле живет более 7 млрд человек. Им нужны чистый воздух, вода, продукты питания, жилье, энергия, различные товары и другие блага. Все это требует неуклонного роста производства, расширения хозяйственной деятельности и вовлечения в ее оборот во всевозрастающих масштабах природных ресурсов. Под воздействием данных процессов меняется среда обитания. Экосистема подвергается постоянному давлению. Возникает реальное противоречие между необходимостью экономического развития и его отрицательным влиянием на природу. Негативные последствия этого противоречия принимают конкретные формы. Ежегодно мировая экономика теряет 5,1 трлн долл. из-за преждевременных смертей от загрязнения воздуха. Это примерно вдвое больше ВВП Великобритании. В бедных государствах дети младше пяти лет умирают из-за загрязнения воздуха в 60 раз чаще, чем в богатых [19, c. 126]. Цель работы: показать, что для решения экологических проблем требуются не отдельные усилия, а целый комплекс мер экономического, административного, организационно-технического и финансового порядка. Метод или методология проведения работы: основополагающим для предпринимаемого исследования является описательный метод, включающий прием наблюдения, сопоставления и обобщения. Результаты: доказать, что экономика, в основе которой лежали традиционные, ресурсопожирающие технологии, развивалась по циклу: «ресурсы – производство – потребление – отходы», однако в настоящий момент необходимо сформировать замкнутую систему: «ресурсы – производство – потребление – ресурсы». Область применения результатов: полученные результаты целесообразно применять экономическими субъектами, осуществляющими природоохранную деятельность, в том числе и экологический надзор.