bluuebunny/crossref_metadata_2025_split huggingface dataset:
PSXII-35 The Periovulatory Endocrine Milieu Affects the Composition of the Oviductal Fluid in Beef Cows
Authors: Gonella-Diaza Angela M , Sponchiado Mariana , Pugliesi Guilherme , Turco Edson G Lo , Binelli Mario 
Published: November 2020

Abstract
Our main objective was to compare the composition of the oviductal fluid (OFL) between cows of high and low receptivity to the embryo. A model for receptivity based on the manipulation of the size of the preovulatory follicle (POF) was used to compare the composition of the OFL. Using this model, it has been proved that the oviduct of high receptivity cows has differences in morphology, gene expression, and extracellular matrix remodeling when compared to low receptivity cows. Cycling, non-lactating, multiparous Nelore cows (n = 14) were presynchronized before receiving cloprostenol (large follicle [LF] group) or not (small follicle [SF] group), along with a progesterone (P4) device on Day (D) ─10. Devices were withdrawn, and cloprostenol administered 42–60 h (LF) or 30–36 h (SF) before GnRH agonist treatment (D0). As a result, higher estrogen concentrations, corpora lutea, and progesterone concentrations were also observed in the LF group in comparison to the SF group. Four days after GnRH-induced ovulation, OFL was collected. Quantitative mass spectrometry was used to determine the concentration of 21 amino acids, 21 biogenic amines, 40 acylcarnitines, 76 phosphatidylcholines, 14 lysophosphatidylcholines, 15 sphingomyelins, 29 hexoses, and 17 prostaglandins and related compounds in the OF. MetaboAnalyst 3.0 was used to identify which metabolites better explained the separation of experimental groups and which could potentially be used as markers of receptivity. After multivariate and PLS analysis, samples of the LF and SF were divided clearly into two non-overlapping clusters. The most influential variables to separate the two groups included: Glutamate, Leucine, four phosphatidylcholines, three lysophosphatidylcholines, and arachidonic acid. Univariate analyses further confirmed these results. There were statistical differences in the concentration of 31 metabolites (P ≤ 0.05) between groups. We concluded that the composition of the OFL is different between cows with contrasting receptivity and fertility status.
PSXII-23 Identification and evaluation of novel fertility traits using automated activity monitor data from commercial dairy herds
Authors: Rosenberg Camila A , Cardoso Diercles F , Malchiodi Francesca , Schenkel Flavio , Baes Christine F 
Published: November 2020

Abstract
The reproductive performance of dairy herds is a determinant factor in their production efficiency and long-term profitability. While the genetic improvement of fertility in cows has been a top breeding objective, its success has been constrained by the low heritability of current traits and the negative impact of management practices on trait measurement accuracy. The recent development and adoption of automated sensor technologies, such as activity monitors, within dairy production systems have provided new opportunities to identify relevant and cost-effective phenotypes of fertility. To date, several activity-based measurements of estrus expression have been associated with fertility outcomes and may, therefore, be suitable predictors of female fertility. The objective of this study was to identify and evaluate novel estrus-related fertility traits using automated activity monitor (AAM) data from commercial dairy herds. A total of 8,730,987 records consisting of 10,529 estrus events were collected between January 2019 and March 2020 from 2,434 cows and 689 heifers of two U.S. commercial herds, where cows were fitted with collar-mounted accelerometers (Heatime, SCR Engineers Ltd., Netanya, Israel). The AAM dataset contained individual bi-hourly activity and rumination patterns, as well as computed fertility index scores, which were used to derive fertility traits related to estrus duration and intensity. The mean number of estrus events per cow and heifer was 4.06 ± 0.04 and 2.63 ± 0.07, respectively, with 1,408 of estruses resulting in pregnancy. Traditional fertility traits were calculated from the breeding record information of 3,255 animals. An estimation of the heritabilities along with genetic and phenotypic correlations between the novel estrus-related fertility traits and traditional fertility traits was undertaken using the program AIREML. Outputs from this project are expected to provide further insight on how to best use AAM measurements to improve selection for reproductive performance in commercial dairy herds.
PSVIII-14 Fifteen months of using litter from recycled manure as bedding for dairy cows: a focus on Klebsiella pneumoniae, Klebsiella variicola and Clostridium perfringens
Authors: Julien Carl 
Published: November 2020

Abstract
In recent years, for economical purposes and to improve well-being conditions of cows, several dairy producers have adopted litter made from recycled manure solids (RMS). However, its use raises several safety issues, particularly with respect to mastitis and other infections. We previously observed cases of mastitis caused by K. pneumoniae and K. variicola, and two deadly infections from C. perfringens from cows on RMS. A herd of Holstein cows, distributed in two barns of about 40 lactating cows, was used. RMS was used in one barn, and wood-chip litter (WCL), in the other. RMS was generated by roller presses separation followed by conditioning at 55°C for 24h. Clean and soiled (used) RMS and WCL samples were collected for 15 months. Similarly, material samples before conditioning were also collected, and all samples were analyzed by viability-qPCR. Milk samples from cows with mastitis and somatic cell count data (SCC) were analyzed. Total and living contents of K. pneumoniae, K. variicola, C. perfringens and Klebsiella spp. were higher in the RMS than the WCL (+1.6–4.2 log10, P &lt; 0.05). Composting 24h at 55°C did not affect total and live levels of C. perfringens, K. pneumoniae, and even increased total and live levels of K. variicola (+0.9 log10, P &lt; 0.05). A seasonal effect or the number of cycles recycling the RMS decreased levels of K. pneumoniae in winter. Overall, RMS had no significant effect on SCC, but increased mastitis incidence (+39%, P &lt; 0.05), compared to WCL. Milk analysis revealed that higher levels of K. pneumoniae found in RMS could contribute to the increase in mastitis cases caused by this pathogen. Data reported from this study are not favorable to the use of an RMS conditioned for 24h at 55°C with dairy cows, apropos the microbiological aspect; however, these results must be confirmed by other studies.
PSVIII-7 Dietary L-arginine supplementation enhances growth performance, intestinal antioxidant capacity, immunity and modulate gut microbiota in yellow feathered chickens
Authors: Ruan Dong , Fouad A M , Fan Qiuli , Jiang Shouqun 
Published: November 2020

Abstract
This study investigated the effects of dietary Arginine (Arg) on performance, intestinal antioxidant capacity, immunity, and gut microbiota in Chinese yellow-feathered chickens. One thousand and two hundred 1-day-old female Qingyuan partridge chickens were randomly assigned to 5 groups with 6 replicates of 40 birds each. Chickens were fed diets with 5 total Arg levels (8.5, 9.7, 10.9, 12.1 and 13.3 g/kg) without antibiotics for 30 D. Average daily feed intake, average daily gain, and feed conversion ratio were improved with dietary Arg levels (P &lt; 0.05). Dietary Arg level had a linear (P &lt; 0.05) or quadratic (P &lt; 0.05) effect on the gene expression of glutathione peroxidase 1, hame oxygenase 1, nuclear factor erythroid 2, linearly and quadraticly increased activities of GSH-Px and total antioxidant capacity in the jejunum and ileum. In addition, relative expression of interleukin-1β and toll-like receptor 4 decreased linearly (P &lt; 0.05) and quadratically (P &lt; 0.05) in the ileum with increasing dietary Arg levels; secretory immunoglobulin A contents were increased. In addition, sequencing data of 16S rRNA indicated that dietary Arg increased the abundances of Firmicutes phylum, Romboutsia and Candidatus Arthromitus genus, while decreased that of Clostridium sensu stricto 1. A diet containing 12.1 g Arg/kg could promote growth performance, intestinal antioxidation, innate immunity and modulate gut microbiota in yellow feathered chickens.
PSI-21 Relationship of captive bolt stunning location with basic tissue measurements and exposed cross-sectional brain area in cadaver heads from market pigs
Authors: Anderson Karly , Ries Elizabeth , Backes Jacob , Bishop Katherine , Boll Miranda , Brantner Ellie , Hinrichs Brady , Kirk Ashlynn , Olsen Hannah , Risius Blake , Bildstein Charles , Vogel Kurt D 
Published: November 2020

Abstract
The objective of this study was to contrast the soft tissue thickness, cranial thickness, total tissue thickness, cross-sectional brain area and bolt–brain contact from the common frontal application of captive bolt euthanasia with the alternative location behind the ear in cadaver swine heads. Twenty-three cadaver heads from pigs that were approximately 136 kg and 6 mo of age were collected from a slaughter establishment following CO2 stunning and assigned to either the FRONTAL (n = 11) or CAUDAL TO PINNA (n = 12) application of the captive bolt. The soft tissue thickness was different (P &lt; 0.0001) between the 2 applications (FRONTAL: 8.3±3.4 mm; CAUDAL TO PINNA: 56.5±3.4 mm). The cranial thickness was different (P &lt; 0.0001) between the applications (FRONTAL: 23.4±2.9 mm; CAUDAL TO PINNA: 26.5±2.9 mm). There was a difference (P &lt; 0.0001) in the total tissue thickness between the 2 applications (FRONTAL: 31.7±3.8 mm; CAUDAL TO PINNA: 73.4±3.8 mm). Cross-sectional area was calculated from images collected immediately after the heads were cut along the plane of bolt travel by bandsaw and was different (P = 0.0028) between the 2 applications (FRONTAL: 25.2±1.3 cm2; CAUDAL TO PINNA: 18.9±1.3 cm2). Bolt–brain contact was also assessed from the images, and a difference (P = 0.0360) between the 2 applications (FRONTAL: 100±10.5%; CAUDAL TO PINNA: 66.7±10.5%) was identified. The results of this study suggest that the FRONTAL application may provide a bolt path with less tissue to travel through when compared with the CAUDAL TO PINNA application for pigs of the approximate age and weight of those in this study. Ultimately, the FRONTAL location may present less risk for the captive bolt euthanasia of swine at market weight at this time. Additional refinement of the CAUDAL TO PINNA procedure and modification to the captive bolt device to penetrate to a suitable depth to ensure brain damage is recommended.
PSI-20 Public perceptions of problematic equine industry sectors and management scenarios
Authors: Melvin Kelly , Ivey Jennie L , Schneider Liesel G , Krawczel Peter 
Published: November 2020

Abstract
The equine industry is highly variable with many different sectors and management practices. To determine how the public views common management practices and discipline-specific areas of the equine industry, an online study was distributed via email and social media over a 6-week period to U.S. residents over the age of 18 (n = 1,372). Survey questions included demographics, industry connection, definition of welfare and equine classification. Respondents were asked to select the most concerning option from a series of management-related scenarios. The production livestock and equine industries were then segmented by species or discipline, respectively, and respondents were asked which sector was most problematic. To analyze the data, frequency tables (Proc FREQ) and multinomial logistic regression (Proc LOGISTIC) were used in SAS 9.4 (Cary, NC) to test the factors associated with likelihood to select a given management scenario from each series (α=0.05). Respondents who were heavily connected to the industry were four times more likely than lightly connected individuals to select that a blanketed horse or unblanketed horse in 30°F weather with unlimited access to food and water equally presented no concern than to say that a blanketed or unblanketed horse in 30°F weather with unlimited access to food and water and were concerning (OR= 4.09; 95%CL: 2.08,8.04). Of the 1,244 respondents who answered, 563 (45%) said that the gaited horse industry is the most problematic equine industry compared to the racing industry (41%) and stock horse industry (8.7%). Understanding how the public perceives the various animal industries and management scenarios in relation to an individual’s connection to the industry, classification of equines and welfare definition is important to assess and improve educational intervention strategies.
PSI-4 Changes in serum cortisol concentrations and cytokine profiles of growing pigs subjected to low-intensity heat stress
Authors: Anderson Lauren E , Holt Jonathan P , van Heugten Eric , Poole Daniel H 
Published: November 2020

Abstract
This study was to determine the impact of low-intensity heat stress on inflammatory biomarkers in serum, ileum, jejunum and lung lavage samples. Pigs (n = 64; 24.6 ± 3.7 kg BW) were housed four per pen in thermoneutral conditions (CON; 23.9°C ± 2.5°C) or constant high ambient temperatures (HS; 28.7°C ± 1.3°C) for 16 d, followed by thermoneutral conditions (24.4°C ± 1.8°C) for both groups through d 21, and subsequent heat stress (28.0°C ± 3.0°C) for both groups through d 30. Respiration rate (P &lt; 0.01) was greater in HS than CON pigs from d 2 through d 15 (mean: 82.54 breaths/min vs. 59.26 breaths/min). HS pigs had greater cortisol concentration (40.1 ng/mL vs. 27.3 ng/mL) than CON on d 2, but lower concentrations on d 22 (32.9 ng/mL vs. 48.9 ng/mL) and d 25 (25.4 ng/mL vs. 42.0 ng/mL; treatment x day: P &lt; 0.001). HS pigs tended to have greater serum IL1β (190.1 vs. 143.4 pg/mL; P = 0.06) and greater serum IL12 concentrations (914.9 vs. 785.4 pg/mL; P &lt; 0.01) than CON pigs. HS pigs tended to have greater ileal mucosa IL6 concentrations than CON pigs (50.4 vs. 30.9 pg/mL; P = 0.08). Jejunal mucosa IL1α concentrations tended (P = 0.10) to be greater for CON than HS pigs (13.5 vs. 9.0 pg/mL) and CON pigs had greater jejunal IL12 concentrations (18.1 vs. 11.8 pg/mL) than HS pigs (P = 0.02). Bronchioalveolar lavage from HS pigs tended (P = 0.10) to have a greater granulocyte-macrophage colony-stimulating factor concentration (6.9 vs. 5.3 pg/mL) than CON pigs and had greater IL4 (7.7 vs. 4.2 pg/mL) and IL12 concentrations (34.8 vs. 24.5 pg/mL) than CON pigs (P = 0.01). Increased inflammatory activity and endocrine stress response occurred during low-intensity heat stress, indicating it may be more detrimental than previously accepted.
PSIV-19 Relationship between liver abscess microbiome of feedlot cattle and soil organic matter microbiome isolated from feedlot pens
Authors: Smith Kathryn E , Bester Zeno , Hubbert Michael E , Loest Clint A 
Published: November 2020

Abstract
Liver abscess (LA) prevalence has increased 15% for beef cattle and 25% for Holstein cattle. The prevalence of liver abscesses in the Central Plains region (CR) is greater than the Southwest region (SR) of the United States. The objective was to evaluate soil organic matter (SOM) microbiome of feedlot pens, associated LA microbiomes, and the impact of breed, location, and tylosin. Eighty-three commercial feedlot pens were sampled for SOM prior to harvesting and LA samples were collected from two commercial packing plants in Tolleson, AZ (SR) and Greely, CO (CR). Liver abscess samples were linked to SOM samples. Amplification and sequencing of the V4 variable region of the 16S rRNA gene was performed using the Illumina MiSeq. Bacterial phyla, genera, and diversity indices were reported for LA and SOM samples. Reported data is from a subset of five pens. The most prevalent genera identified in liver abscesses were Fusobacterium (64.2%) and Bacteroides (18.6%). Predominant SOM genera were Atopostipes (13%) and Clostridium (10%). Phylum SOM Shannon Weiner Index (SWI) was lower for the CR compared to the SR (0.034 vs. 0.174; P &lt; 0.05), indicating a dominant bacterium in the CR. Atopostipes and Clostridium comprised 48% of the CR SOM, explaining the lower SWI. The SWI of SOM and liver abscesses of Holsteins are lower (P &lt; 0.05) compared to beef counterparts. Additionally, Clostridium, Fusobacterium, and Atopostipes populations were greater (P &lt; 0.05) in SOM of Holstein pens than beef pens. Genus richness was lower (P &lt; 0.05) for both SOM and liver abscesses from pens of cattle fed tylosin vs. no tylosin. There is a negative correlation between SOM SWI and LA% (R2 = -0.49; P = 0.03) and LA severity (A+) (R2 = -0.44; P = 0.05). In conclusion, SOM may serve as a potential vector for liver abscesses.
PSIV-11 Effects of very low-dose antibiotics on gene expression profiles in ileal mucosa of weaned pigs infected with a pathogenic E. coli
Authors: Kim Kwangwook , Jang Sungbong , Liu Yanhong 
Published: November 2020

Abstract
Our previous studies have shown that supplementation of low-dose antibiotic growth promoter (AGP) exacerbated growth performance and systemic inflammation of weaned pigs infected with pathogenic Escherichia coli (E. coli). The objective of this experiment, which is extension of our previous report, was to investigate the effect of low-dose AGP on gene expression in ileal mucosa of weaned pigs experimentally infected with F18 E. coli. Thirty-four pigs (6.88 ± 1.03 kg BW) were individually housed in disease containment rooms and randomly allotted to one of three treatments (9 to 13 pigs/treatment). The three dietary treatments were control diet (control), and 2 additional diets supplemented with 0.5 or 50 mg/kg of AGP (carbadox), respectively. The experiment lasted 18 d [7 d before and 11 d after first inoculation (d 0)]. The F18 E. coli inoculum was orally provided to all pigs with the dose of 1010 cfu/3 mL for 3 consecutive days. Total RNA [4 to 6 pigs/treatment on d 5; 5 to 7 pigs/treatment on 11 post-inoculation (PI)] was extracted from ileal mucosa to analyze gene expression profiles by Batch-Tag-Seq. The modulated differential gene expression were defined by 1.5-fold difference and a cutoff of P &lt; 0.05 using limma-voom package. All processed data were statistically analyzed and evaluated by PANTHER classification system to determine the biological process function of genes in these lists. Compared to control, supplementation of recommended-dose AGP down-regulated genes related to inflammatory responses on d 5 and 11 PI; whereas, feeding low-dose AGP up-regulated genes associated with negative regulation of metabolic process on d 5, but down-regulated the genes related to immune responses on d 11 PI. The present observations support adverse effects of low-dose AGP in our previous study, indicated by exacerbated the detrimental effects of E. coli infection on pigs’ growth rate, diarrhea and systemic inflammation.
PSIV-25 Effect of Various Handling Methods of Plasma and Serum Samples on Pregnancy Associated Glycoprotein Concentrations
Authors: Wesson Grace M , Fernandez Lohana , Poole Rebecca K , Franco Gessica A , Reese Sydney T , Pohler Ky G 
Published: November 2020

Abstract
Pregnancy associated glycoproteins (PAG) can be used as a biomarker for early pregnancy diagnosis, so accurate and consistent PAG detection is critical. The objective of this study was to determine if plasma and serum PAG concentrations were altered when centrifugation occurred at different times post-collection, when subjected to repeated freezing and thawing, and when monoclonal antibodies were kept in frequently or infrequently opened containers. Plasma (n = 4) and serum (n = 4) samples were collected from two open cows and two pregnant cows 28 days after artificial insemination. Pregnancy status was determined via transrectal ultrasonography. Plasma and serum samples were evenly separated and either centrifuged on the day of collection, or placed at 4°C and centrifuged the next day. An in-house PAG ELISA was performed on all samples before freezing (NOTHAW), after being frozen for one week (INTACT), after one freeze/thaw cycle (THAW1), two freeze/thaw cycles (THAW2), and three freeze/thaw cycles (THAW3). Data were analyzed using one-way ANOVA (GLM procedure, SAS 9.4). All samples from open cows were below the baseline of the assay. For pregnant cows, plasma samples had greater PAG concentrations than serum samples (11.84 vs 3.30 ± 0.66 ng/mL, respectively, P &lt; 0.05). No differences were observed for day of centrifugation in both plasma and serum samples (P = 0.50 and P = 0.60, respectively) and in handling of monoclonal antibodies (P = 0.90). Freezing and thawing did not impact PAG concentrations in plasma samples (P = 0.19), but did alter serum concentrations (P = 0.01). Specifically, THAW1 (1.98 ng/mL) and THAW2 (1.42 ng/mL) serum PAG concentrations were lower compared to NOTHAW, THAW3, and INTACT samples (4.66, 4.85, and 3.57 ng/mL, respectively). Based on these data, plasma yields more consistent results than serum, even after several freeze-thaw cycles, and handling of monoclonal antibodies or time of centrifugation has no significant effect on measured PAG.
PSVII-20 Supplementing pHix-Up® to feedlot cattle consuming a corn-based finishing diet
Authors: Colombo Eduardo , Cooke Reinaldo F , Brandão Alice , Souza Rodrigo , Duclos Julie , Schubach Kelsey , Pohler Ky G 
Published: November 2020

Abstract
This experiment evaluated the inclusion of a rumen-buffering agent on performance and carcass traits of feedlot cattle. Angus-influenced steers and heifers (n = 64 animals/sex) were ranked by sex and body weight (BW) into 1 or 16 drylot pens. Pens were randomly assigned to receive a corn-based diet without (CON = 8 pens) or with the inclusion (SUPP = 8 pens) of pHix-Up® (magnesium-based product; Timab, Dinard, France) at 0.50% of diet dry matter. Both dietary treatments contained monensin Na (360 mg animal/daily) but did not contain tylosin. Two animals per pen were equipped with an intraruminal bolus (smaXtec Premium Bolus, smaXtec Animal Care GmbH, Graz, Austria) that recorded pH every 15 min. Cattle received treatments for 128 d until slaughter. No differences (P ≥ 0.22) between SUPP and CON cattle were noted for BW gain (0.93 vs. 0.97 kg/d, respectively), feed intake (7.51 vs. 7.78 kg/animal daily, respectively), or feed efficiency (0.121 vs. 0.125 kg/kg, respectively). Rumen pH was greater in SUPP vs. CON cattle during 33% of the time (e.g. 6 h of the day; mean pH of 5.87 vs. 5.80, respectively). No treatment differences were noted (P ≥ 0.12) for carcass merit. Incidence of cattle with liver abscess upon slaughter was greater in CON vs. SUPP (16.4 vs. 4.7%, respectively), including incidence of severe liver abscess (A+; 8.2 vs. 0.0%, respectively). These results indicate that pHix-Up® inclusion into a corn-based finishing diet improved rumen pH without benefiting cattle productive responses. It should be noted that average temperature-humidity index during this experiment was 78, which hindered feed intake and potential benefits of pHix-Up® to rumen pH and cattle performance. Nonetheless, pHix-Up® inclusion substantially reduced the incidence of liver abscess, resulting no severe diagnoses. Hence, additional research is warranted to further explore the benefits of pHix-Up® to feedlot cattle.