bluuebunny/crossref_metadata_2025_split huggingface dataset:
The diagnostic accuracy of CT based “Banner cloud sign” for dural ossification in patients with thoracic ossification of the ligamentum flavum: a prospective, blinded, diagnostic accuracy study protocol
Authors: Chen Guanghui , Zhang Baoliang , Tao Liyuan , Chen Zhongqiang , Sun Chuiguo 
Published: May 2020

Abstract
Background

Ossification of the ligamentum flavum (OLF) is the most common cause of thoracic spinal stenosis, and responds poorly to conservative treatment, making surgery the only effective method. The existence of dural ossification (DO) makes surgery challenging and increases the risk of intra-/post-operative complications. To date, several methods have been proposed to identify DO, but either the diagnostic accuracy is low or the feasibility is poor. Therefore, the aim of this study is to propose a new imaging sign (Banner cloud sign, BCs), evaluate its diagnostic accuracy in the diagnosis of DO, and provide reliable evidence-based data for its application in clinical practice.
Methods

This will be a prospective, blinded, diagnostic accuracy study to assess and compare the accuracy of BCs in the diagnosis of DO with other radiologic signs (Tram track sign (TTs) and Comma sign (Cs)). A total of 120 patients who were diagnosed with OLF and underwent decompression in Peking University Third Hospital from January 2018 to June 2019 will be enrolled. Patients’ medical records and imaging data will be retrieved from the hospital database server. An observational group consisting of six spine surgeons of different seniority and two epidemiological researchers will read patients’ images to determine whether there are typical imaging signs and thus the presence of DO. Surgical records will be reviewed to confirm the presence of DO, and the results will serve as the reference standard for estimating accuracy. The primary outcome of the study is to determine the accuracy of BCs for DO diagnosis, and the secondary outcome is to compare the sensitivity, specificity, area under the ROC curve and inter-observer reliability of each imaging sign. The time taken and level of confidence in DO diagnosis of each observer will also be compared.
Discussion

This study represents the first large investigation of the diagnostic value of “BCs”, “TTs” and “Cs” in the diagnosis of DO, and will provide convincing evidence about their clinical application.
Trial registration:

Registered on 29 February 2020. Trial number is ChiCTR2000030380
French Adaptation of the Activity Impairment Assessment Score in Order to Assess the Activity Impairment of Women Presenting with Acute Uncomplicated Cystitis in Primary Care: Translation and Psychometric Validation.
Authors: VINCENT Yves-Marie , Duhart Mélanie , Su Alain Chen Yen , Micoulau-Franchi Jean-Arthur , Conort Guillaume 
Published: May 2020

Abstract
PurposeThe Activity Impairment Assessment (AIA) is a 5 items questionnaire exploring the impact of acute cystitis on daily activities. It’s a self-administrated tool easy to use in primary care studies. The purpose of this study is to translate and validate a French version of the AIA: the AIA-fr.MethodsWe use a forward-backward translation before to analyze the psychometric properties of the French version. We proceeded to a quantitative, prospective, observational multicentric study. King Health Questionnaire and Ditrovie scale were used to determine convergent and divergent validity. Following an inclusion visit, each file was completed by the patient on day 0, 3 and 5.ResultsFifty-two files were received by the investigator of which two empty files. In the end, 50 files were included in the study. There was a 100% response rate for the AIA-fr on days 0, 3 and 5. Cronbach’s alpha was calculated at 0.87 with a 95% confidence interval between 0.78 and 0.92. We found that this coefficient was not improved by removing one of the items (Cronbach’s alpha 0.81–0.87). There is a negative correlation (p = 0.01 Rs = − 0.35) between the total AIA-fr score and the number of days between symptom onset and consultation (day 0). This implies that the higher the AIA-fr score the sooner women will seek medical care.ConclusionsThe French version of the AIA is a psychometrically acceptable self-report questionnaire for measuring the impact of acute cystitis on daily activities. It’s the first French tool evaluating this impact.
Relationship between Serum Bilirubin Level and the Prevalence of Lung Cancer in Smokers
Authors: Liu Weilin , Fu Tian , Wei Le , Liu Chun , Li Mohan 
Published: May 2020

Abstract
Background

The correlation between serum bilirubin level and multiple respiratory system diseases including lung cancer has been reported. We aimed to explore the relationship between serum bilirubin level and the prevalence of lung cancer among smokers in China.
Methods

We designed a retrospective case-control study and enrolled 266 lung cancer patients and 266 matched controls from The Third Xiangya Hospital of Central South University from February 2011 to May 2017. Data including diagnosis, serum bilirubin levels and smoking history were collected, which consistently showed significant differential in cases and controls.
Results

Lower total and indirect bilirubin level is correlated with smoking but they can be recuperating after cessation. The average bilirubin level in lung cancer patients was lower than matched controls. Low indirect bilirubin level within normal range is the risk factor of lung cancer in smokers (OR = 2.710). Of note, smokers whose serum bilirubin lower than 7.88 µmol / L has a higher risk of lung cancer in Chinese adults.
Conclusions

Serum indirect bilirubin level would be a screening predictor to identify higher risk smokers of lung cancer.
Prediction of potential prognostic biomarkers in metastatic prostate cancer based on a competing endogenous RNA regulatory network
Authors: Luo Liang , Tao Wen , He Tao , li liao-yuan 
Published: May 2020

Abstract
Background

Recently, studies on competing endogenous RNA (ceRNA) networks were becoming prevalent, and circular RNAs (circRNA) have crucial implications for the development and progression of carcinoma. However, studies relevant to metastatic prostate cancer (mPCa) are scanty.
Methods

RNA-Seq data was obtained from MiOncoCirc database and Gene Expression Omnibus (GEO). Differential expression patterns of RNAs were examined using R packages. Circular RNA Interactome, miRTarBase, miRDB and TargetScan were applied to predicting the corresponding relation between circRNAs, miRNAs and mRNAs. The Gene Ontology (GO) annotations were performed to present related GO terms, and Gene Set Enrichment Analysis (GSEA) tools were applied for pathway annotations. Moreover, survival analysis was conducted for hub genes.
Results

We found 820 circRNAs, 81 miRNAs and 179 mRNAs, which were distinguishingly expressed between primary prostate cancer (PCa) and mPCa samples. A ceRNA network including 45 circRNAs, 24 miRNAs and 56 mRNAs was constructed. Besides, the protein-protein interaction (PPI) network was built up, and 10 hub genes were selected by using CytoHubba application. Among the 10 hub genes, survival analysis showed ITGA1, LMOD1, MYH11, MYLK, SORBS1 and TGFBR3 were significantly connected with disease-free survival (DFS).
Conclusion

The circRNA-mediated ceRNA network provides potential prognostic biomarkers for metastatic prostate cancer .
Mathematical study for the phase-based transmissibility of a novel COVID-19 Coronavirus
Authors: HAJJI Miled EL , SAYARI Sayed , ZAGHDANI Abdelhamid 
Published: May 2020

Abstract
In this paper, a mathematical dynamical system modeling a SEIRW model of infectious disease transmission for a transmissibility of a novel COVID-19 Coronavirus is studied. A qualitative analysis such as the local and global stability of equilibrium points is carried out.It is proved that if $\R \leq 1$, then the disease-free equilibrium is globally asymptotically stable and if $\R > 1$, then the disease-persistence equilibrium is globally asymptotically stable.
COVID-19 is a risk factor for severe liver damage for patients with intrahepatic cholestasis of pregnancy: a case report
Authors: Ai Ling , Wei Jia , Wang Shaofang , Tan Xi , Yan Li , Liu Shangkun , Yang Liu , Luo Ailin , Xu Hui 
Published: May 2020

Abstract
Background: Patients with intrahepatic cholestasis of pregnancy (ICP) may present with slight liver damage. In the global outbreak, the number of pregnant women infected with coronavirus disease 2019 (COVID-19) is increasing. For the pregnant patients with ICP, COVID-19 may cause severe liver damage.Case presentation: A 31-year-old pregnant woman was admitted with fever and respiratory symptoms to Tongji Hospital in Wuhan amid the outbreak of COVID-19. Her chest CT scan showed an infection with viral pneumonia as multiple ground glass opacities in both lungs were spotted. Laboratory tests revealed increased white blood cell (WBC) count and decreased lymphocyte count. The levels of serum total bile acid (TBA) were highly elvated. So were the indices of liver function, including alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin (TBIL), direct bilirubin (DBIL), alkaline phosphatase (AKP), 𝛄-glutamyltranspeptidase (𝛄-GT), and lactate dehydrogenase (LDH). The patient was later diagnosed of COVID-19 with comorbid ICP, presenting severe liver damage. Through timely termination of pregnancy and effective treatments, the prognoses of the patient and the fetus were well improved.Conclusions: This case highlights that COVID-19 may be a risk factor of severe liver damage for patients with ICP.Timely termination of pregnancy and effective symptomatic treatments are helpful to improve the progonosis.
Annual runoff prediction in the source area of the Yellow River based on structure change co-integration theory
Authors: Zhang Jinping , Li Hongbin , Sun Bin , Fang Hongyuan 
Published: May 2020

AbstractAiming at revealing the co-integration under structural change in the long-run relationship between rainfall and runoff time series at Tangnaihai Hydrological Station in the source area of the Yellow River, and improving the accuracy of annual runoff prediction, co-integration theory and structure change co-integration theory are introduced respectively. The error correction models of rainfall and runoff in these two cases are constructed. The results show that reservoir construction and climate change can cause structure change in the long-run relationship between rainfall and runoff in the source area of the Yellow River. The breakpoints appeared in 1989 and 2002, in which the breakpoint in 1989 is mainly effected by reservoir construction while in 2002 it is effected by rainfall changes. Meanwhile, the error correction model with structural change shows that the impact of rainfall on runoff decreases from 1989 but increases from 2002. Finally, for the prediction of runoff in the next five years, the mean absolute percentage errors of the prediction models without and with breakpoints are 11.04% and 7.08% respectively, and this shows that the error correction model with structural change has the higher runoff prediction accuracy.
Laudacja wygłoszona 11 grudnia 2015 r. podczas uroczystości Jubileuszu Pana Profesora Jakuba Pokory przez prof. Christine Moisan-Jablonski
Authors: Moisan-Jablonski Christine 
Published: May 2020

Laudacja wygłoszona 11 grudnia 2015 r. podczas uroczystości Jubileuszu Pana Profesora Jakuba Pokory przez prof. Christine Moisan-Jablonski.
The laudation made by prof. Christine Moisan-Jablonski at the Jubilee of Prof. Jakub Pokora on 11th of December 2015.
The Effect of Bisphosphonates on Managing Osteoporosis After Spinal Cord Injury: A Meta-Analysis
Authors: Xinghua Ji , Junjie Wang , Yao Guo , Peng Shang , Jianzhong Huo 
Published: May 2020

Background:
The increased bone loss after spinal cord injury (SCI) is associated with an increase in
the morbidity and mortality of fragility fractures, which can constitute a substantial cost to health care systems.
Bisphosphonates (BPs) are now the principal class of medications used for osteoporosis.

Objective:
To demonstrate the effect of BPs on treating osteoporosis after SCI.

Methods:
A comprehensive search in PubMed, EMBASE, Web of Science and Cochrane Central databases was
undertaken for randomized controlled trials (RCTs), exploring the effect of BPs on osteoporosis after SCI. The
primary outcome measures were the BMD of different locations, serum bone turnover marker levels, serum biochemistry
marker levels and adverse effect (AE) risks. The final search was performed in September 2019. Reporting
was carried out according to PRISMA Guidelines.

Results:
Six RCTs were included. A total of 147 patients met the inclusion criteria. BPs were found to statistically
prevent bone loss in the total hip, femoral neck and trochanter at the 6- and 12-month follow-up points and
to increase the BMD of the lumbar spine at the 12-month follow-up time point. BPs had no clear effect on serum
PINP or serum calcium levels at the 12-month follow-up time point.

Conclusion:
BP therapy may prevent bone loss in the lumbar spine and hip when administered early after SCI
and has relatively high safety.
MicroRNA-126: Dual Role in Angiogenesis Dependent Diseases
Authors: Nammian Pegah , Razban Vahid , Tabei Seyed Mohammad Bagher , Asadi-Yousefabad Seyedeh-Leili 
Published: May 2020

Background:
MicroRNA-126, a microRNA implicated in blood vessel integrity and angiogenesis is
significantly up/down regulated in different physiological and pathological conditions related to angiogenesis
such as cardiovascular formation and angiogenesis dependent diseases. MicroRNA-126 plays a critical role in
angiogenesis via regulating the proliferation, differentiation, migration, and apoptosis of angiogenesis related
cells such as endothelial cells.

Objective:
The aim of this review is to investigate the molecular mechanisms and the effects of microRNA-126
on the process of angiogenesis in pathophysiological conditions.

Methods:
To conduct this review, related articles published between 2001 and 2019 were collected from the
PubMed, Web of Science, Google Scholar, Scopus and Scientific Information Database using search terms such
as microRNA-126, angiogenesis, cardiovascular disorders, hypoxia, VEFG-A, endothelial cells, VEGF pathway,
and gene silencing. Then, the qualified articles were reviewed.

Result:
MicroRNA-126 regulates the response of endothelial cells to VEGF, through directly repressing multiple
targets, including Sprouty-related EVH1 domain-containing protein 1 (SPRED1) and phosphoinositol-3 kinase
regulatory subunit 2 (PIK3R2/p85-b). MicroRNA-126 -3p and microRNA-126 -5p have cell-type and strandspecific
functions and also various targets in angiogenesis that lead to the regulation of angiogenesis via different
pathways and consequently diverse responses.

Conclusion:
MicroRNA-126 can bind to multiple targets and potentially be both positive and negative regulators
of gene expression. Thus, microRNA-126 could cause the opposite biological effects depending on the context.
As a result, understanding the different cellular pathways through which microRNA-126 regulates angiogenesis
in various situations is a critical aspect in the development of novel and effective treatments for diseases with
insufficient angiogenesis.
Annurca Apple Biophenols’ Effects in Combination with Cisplatin on A549 Cells
Authors: Boccellino Mariarosaria , Quagliuolo Lucio , D’Angelo Stefania 
Published: May 2020

Background:
The role of a healthy and balanced diet in cancer prevention should never be
underestimated; some nutritional factors can reduce the side effects of chemotherapy or radiotherapy
and contribute to increasing its effectiveness. Therefore, in recent years, it has been thought to associate
the administration of antioxidants to the chemotherapy approach that can protect non-tumor
cells from the cytotoxic action of these drugs. However, the protective action of these substances
could also limit the chemotherapy effects against the neoplastic cells themselves.

Objective:
In this context, the goal of this work was to test the viability of cultured human non-small
lung cancer A549 cells in response to the combined administration of cisplatin (CDDP) and polyphenols.
In particular, Annurca apple flesh polyphenol extract (AFPE) action was examined.

Methods:
A549 cells were treated with AFPE alone or in combination with CDDP and then cell viability
was measured by the MTT assay. The effects of constituent polyphenols (+)-catechin, (-)-
epicatechin, and caffeic acid) in AFPE were also evaluated. The cell morphology was observed by an
inverted phase-contrast microscope.

Results:
CDDP reduced A549 cell viability in both concentration- and time-dependent manners. Polyphenols
and CDDP co-administration did not interfere with the CDDP efficacy, in fact, the cellular
vitality was found to be similar to that detected in the samples treated with CDDP alone.

Conclusion:
In conclusion, the co-administration of AFPE with CDDP does not interfere with its
chemotherapy efficacy. Therefore, Annurca apple could be a good candidate to act as an antioxidant
and potentially reduce the side effects of CDDP therapy, although the joint effect of AFPE and
CDDP on normal cells is still unclear. More studies will be needed to analyze the molecular mechanism
of AFPE and in vivo studies will also be needed to verify the anticancer effects. These findings
may represent a starting point for the design of new clinical trials for use in cancer treatment.
The Shift of HbF to HbA under Influence of SKA2 Gene; A Possible Link between Cortisol and Hematopoietic Maturation in Term and Preterm Newborns
Authors: Ijabi Janat , Afrisham Reza , Moradi-Sardareh Hemen , Roozehdar Parisa , Seifi Fatemeh , Sahebkar Amirhossein , Ijabi Roghayeh 
Published: May 2020

Background:
We hypothesized that the SKA2 gene can convert hemoglobin F to A leading
to the maturity of the hematopoietic system by glucocorticoid hormone; so, the present study aimed to
investigate the health outcome of newborns by using the effect of SKA2 gene on hematopoietic maturation.

Methods:
At first, 142 samples were divided into term and preterm. After sampling from the umbilical
cord blood, the expression of SKA2 genes and HbA and F were evaluated by quantitative RT-PCR.
The blood gases were measured by Campact 3 device. Finally, the cortisol level was measured by
ELISA method and HbA and F levels were investigated by capillary electrophoresis.

Results:
The blood gases and Apgar scores were more favorable in term newborns (P <0.001). Levels
of protein/expression of HbF in newborns with Apgar score greater than 7 was lower than that of the
newborns with Apgar score below 7 (P <0.001). Cortisol and HbA levels were considerably higher in
term newborns compared to the preterm ones (P <0.001). In the preterm and term groups, SKA2 gene
expression had a positive and significant relationship with cortisol and HbA levels as well as a negative
relationship with the HbF level. In the preterm group, a positive and significant relationship was
observed between the expression of SKA2 and HbF genes.

Conclusion:
The results revealed that the SKA2 gene affected hematopoietic maturation in preterm
and term newborns and the health outcome of newborns improved by increasing HbA level.
Lead Anti-Obesity Compounds from Nature
Authors: Dahiya Vinesh , Vasudeva Neeru , Sharma Sunil , Kumar Ashok , Rowley David 
Published: May 2020

Background :
Obesity has become a global issue, leading to increased risk of metabolic
syndrome, which encompasses diabetes, cardiovascular disease, stroke, hypertension, and certain cancers.
However, obesity is difficult to control through diet and exercise alone, as they are difficult to
implement.

Objective:
The objective of this review is to elucidate the active constituents that can be obtained from
various natural sources that act as anti-obesity agents. Due to the global rise in the prevalence of obesity,
an urgent need to prevent and control it has arisen.

Methods:
For this review, we compiled information about natural anti-obesity products through an
electronic search of the articles available via PubMed, Scopus, and other internet sources for the period
1975-2019 and included our own research. We analyzed and organized data on various natural products
in popular use in addition to relevant pharmacognostic and biological studies. The products’
mechanisms of action were also investigated.

Conclusion:
Consumption of diets that include high amounts of active anti-obesity natural compounds
is a promising strategy for the suppression of lipid accumulation and adipogenesis in obese individuals.
Internet-Based Self-Management Support After High-Altitude Climate Treatment for Severe Asthma: Randomized Controlled Trial
Authors: Beerthuizen Thijs , Rijssenbeek-Nouwens Lucia H , van Koppen Sophia M , Khusial Rishi J , Snoeck-Stroband Jiska B , Sont Jacob K 
Published: May 2020

Background
In patients with severe asthma, high-altitude climate treatment has been shown to improve asthma control. However, asthma symptoms and limitations may increase after finishing inpatient rehabilitation programs and returning to sea level.

Objective
We assessed the effectiveness of a patient-tailored, internet-based, self-management strategy in addition to usual care after finishing high-altitude climate treatment.

Methods
We performed a randomized controlled trial with a 1-year follow-up in patients from a high-altitude asthma center in Davos, Switzerland. At the end of a 12-week multidisciplinary rehabilitation program, 62 adults with asthma were randomized to receive either internet-based self-management support in addition to usual care (n=33) or usual care only after discharge (n=29). The endpoints were changes in asthma-related quality of life according to the Asthma Quality of Life Questionnaire (AQLQ) (a higher score is better) and asthma control according to the Asthma Control Questionnaire (ACQ) (a lower score is better), with a minimally important difference of 0.5 points for both.

Results
Asthma-related quality of life and asthma control declined over time in the usual care strategy group, whereas there was a slower decline in the internet-based strategy group. For both endpoints, mixed-model analysis showed a significant positive effect in favor of internet-based self-management during follow-up (mean AQLQ score difference 0.39, 95% CI 0.092-0.69; P=.01 and ACQ score difference −0.50, 95% CI −0.86 to −0.15; P=.006), which was prominent among patients with uncontrolled asthma at discharge (AQLQ score difference 0.59, 95% CI 0.19-0.99; P=.003 and ACQ score difference −0.73, 95% CI −1.18 to −0.28; P=.002).

Conclusions
Internet-based self-management support was associated with a smaller decline in quality of life and asthma control as compared with usual care, especially in patients with lower asthma control, after completion of high-altitude climate treatment. Internet-based self-management support in adults with severe asthma seems feasible and effective to maintain quality of life and asthma control.

Trial Registration
The trial is registered in the Netherlands Trial Register (NTR1995).