bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prognostic significance of infectious episodes occurring during first‐line therapy for diffuse large B‐cell lymphoma ‐ A nationwide cohort study
Authors: Clausen Michael R. , Ulrichsen Sinna P. , Juul Maja B. , Poulsen Christian B. , Iversen Brian , Pedersen Per T. , Madsen Jakob , Pedersen Robert S. , Josefsson Pär L. , Gørløv Jette S. , Nørgaard Mette , d'Amore Francesco 
Published: April 2020

AbstractInfections during first‐line therapy for DLBCL are often associated with chemotherapy dose reductions and increased mortality. Systemic infections have also been suggested as beneficial promotors of immunological responses. However, whether there is an association between the timing of an infectious episode and outcome during treatment has not yet been clarified. We investigated how the occurrence and timing of infectious episodes during the first line of treatment for “de novo” DLBCL influenced patient outcome. We used data on DLBCL patients from the Danish Lymphoma Registry, the Danish National Patient Registry, and the Danish National Pathology Registry. Infections were categorized according to type (ICD‐10) and time of occurrence after treatment start. “Early” infections were defined as occurring between days 7 and 42 and “late” infections between days 100 and 150 from treatment start. Patients experiencing both “early and late” infections were categorized separately. We used multivariable Cox regression and Kaplan‐Meier estimates to assess the association between infections and survival adjusting for NCCN‐IPI, sex, comorbidity, and rituximab treatment. We identified 3546 patients, median age 65 years (IQR 56,73). Infectious episodes occurred in 1171 (33%) patients, of which 666 had “early,” 303 “late,” and 202 both “early and late” events. Patients without registered infections had a 5‐year overall survival (OS) rates of 74%. Those with “early,” “late,” or “early+late” had 5‐year OS of 65%, 62%, and 53%, respectively. Compared with patients without any registered infections, hazard rate ratios (HR) were 1.24 (95% CI 1.05‐1.47), 1.32 (95% CI 1.06–1.63), and 1.59 (95% CI 1.27‐2.00), respectively, in the multivariable model. We observed that infectious episodes during first‐line treatment for “de novo” DLBCL occurred in 44% of the patients. Irrespective of timing, patients with infectious episodes had an inferior outcome compared to those without. Outcome patterns were similar for patients registered with sepsis.
Effect of venlafaxine dosage, valproic acid concentration, sex, and age on steady state dose‐corrected concentrations of venlafaxine and O‐desmethylvenlafaxine: A retrospective analysis of therapeutic drug monitoring data in a Chinese population
Authors: Wang Zhan‐zhang , Deng Shu‐hua , Lu Hao‐yang , Li Lu , Zhu Xiu‐qing , Hu Jin‐qing , Xie Huan‐shan , Chen Hong‐zhen , Chen Yu‐qing , Zhang Ming , Fang Zi‐yan , Wen Yu‐guan , Shang De‐wei 
Published: April 2020

AbstractPurposeThis study aimed to investigate the influence of diagnosis, body weight, sex, age, smoking, formulations, and concomitant drugs on steady‐state dose‐corrected serum concentrations (C/D) of venlafaxine (VEN) and O‐desmethylvenlafaxine (ODV).MethodsA retrospective analysis of therapeutic drug monitoring (TDM) was carried out. Patients' demographic data, therapeutic regimens, and concentrations were collected.ResultsWe included 91 verified samples from 80 patients. Females had by average 13% smaller body weight, 50% higher C/D of VEN, and VEN + ODV and 25% smaller ODV/VEN than males. Patients >60 years had by average 33–59% higher C/D levels of ODV and VEN + ODV than younger patients. The concomitant use of valproic acid caused an average 51% higher C/D of ODV and a 2.2‐fold larger ODV/VEN, while clozapine was related with 40% smaller ratio of ODV/VEN and 38% lower C/D levels of ODV. Positive correlations were detected between valproic acid concentrations and the C/D of VEN and VEN + ODV. In a multiple linear regression analysis, variance in the C/D of VEN + ODV was partly attributed to the daily dose of VEN, sex, age and valproic acid concentration.ConclusionOur results suggested daily dose of VEN, sex, age, and valproic acid as indicators for the C/D of VEN + ODV in Chinese patients. TDM as a valuable tool was suggested in elderly female patients and patients receiving polypharmacy.
Reducing data acquisition for light‐sheet microscopy by extrapolation between imaged planes
Authors: Shemesh Ziv , Chaimovich Gal , Gino Liron , Ozana Nisan , Nylk Jonathan , Dholakia Kishan , Zalevsky Zeev 
Published: April 2020

AbstractLight‐sheet fluorescence microscopy (LSFM) is a powerful technique that can provide high‐resolution images of biological samples. Therefore, this technique offers significant improvement for three‐dimensional (3D) imaging of living cells. However, producing high‐resolution 3D images of a single cell or biological tissues, normally requires high acquisition rate of focal planes, which means a large amount of sample sections. Consequently, it consumes a vast amount of processing time and memory, especially when studying real‐time processes inside living cells. We describe an approach to minimize data acquisition by interpolation between planes using a phase retrieval algorithm. We demonstrate this approach on LSFM data sets and show reconstruction of intermediate sections of the sparse samples. Since this method diminishes the required amount of acquisition focal planes, it also reduces acquisition time of samples as well. Our suggested method has proven to reconstruct unacquired intermediate planes from diluted data sets up to 10× fold. The reconstructed planes were found correlated to the original preacquired samples (control group) with correlation coefficient of up to 90%. Given the findings, this procedure appears to be a powerful method for inquiring and analyzing biological samples.
Frizzled 6 disruption suppresses osteoblast differentiation induced by nanotopography through the canonical Wnt signaling pathway
Authors: Abuna Rodrigo Paolo Flores , Oliveira Fabiola Singaretti , Adolpho Leticia Faustino , Fernandes Roger Rodrigo , Rosa Adalberto Luiz , Beloti Marcio Mateus 
Published: April 2020

AbstractThis study aimed to investigate if wingless‐related integration site (Wnt) signaling participates in the high osteogenic potential of titanium with nanotopography (Ti‐Nano). We showed that among the several components of the Wnt signaling pathway, Frizzled 6 (Fzd6) was the transcript most intensely modulated by nanotopography compared with the untreated Ti surface (Ti‐Machined). Then, we investigated whether and how Fzd6 participates in the regulation of osteoblast differentiation caused by nanotopography. The Fzd6 silencing with CRISPR–Cas9 transfection in MC3T3‐E1 cells induced a more pronounced inhibition of osteoblast differentiation of cells cultured on nanotopography than those cultured on Ti‐Machined. The analysis of the expression of calcium‐calmodulin‐dependent protein kinase II and β‐catenin demonstrated that Fzd6 disruption inhibited the osteoblast differentiation induced by Ti‐Nano by preventing the activation of Wnt/β‐catenin but not that of Wnt/Ca2+ signaling, which is usually triggered by the receptor Fzd6. These findings elucidate the biological function of Fzd6 as a receptor that triggers Wnt/β‐catenin signaling and the cellular mechanisms modulated by nanotopography during osteoblast differentiation.
Aurora kinase inhibitor restrains STAT5‐activated leukemic cell proliferation by inducing mitochondrial impairment
Authors: Wang Jin‐Xing , Zhang Ling , Huang Ze‐Wei , Zhang Xue‐Ning , Jiang Yan‐Yan , Liu Fang‐Jie , Long Liang , Xue Man‐Jie , Lu Gui , Liu Quentin , Long Zi‐Jie 
Published: April 2020

AbstractCurrent chemotherapy regimens on acute myeloid leukemia (AML) still have some drawbacks, such as intolerance and drug resistance, which calls need for the development of targeted therapy. Signal transducer and activator of transcription 5 (STAT5) is often overexpressed or abnormally activated in leukemia and involved in cell self‐renewal, proliferation, and stress adaptation. Overexpressed Aurora A (AURKA) is associated with poor prognosis in tumors, and inhibitors against AURKA are already in clinical trials. However, it has rarely been reported whether AURKA inhibitors restrain STAT5‐activated leukemia cells. In this study, we constructed STAT5 constitutively activated (cS5) cells and found that STAT5 promoted cell proliferation and colony formation. Moreover, cS5 cells showed elevated reactive oxygen species (ROS) and adenosine triphosphate (ATP) levels, which indicated higher mitochondrial metabolism in cS5 cells. A novel AURKA inhibitor AKI604 was synthesized and showed significant inhibitory effects to the proliferation and colony formation in both STAT5 constitutively activated and nonactivated AML cells. AKI604 induced mitochondrial impairment, leading to the disruption of mitochondrial membrane potential and the elevation of ROS as well as cellular calcium (Ca2+) levels. AKI604 could also decline basal oxygen consumption rate and ATP biosynthesis, indicating the damage of oxidative phosphorylation. Furthermore, AKI604 exhibited significant antitumor effect in the HL‐60 cS5 xenograft model of the BALB/c nude mice without an obvious influence on mice body weight and other healthy indicators. This study suggested that AKI604 was a potential strategy to overcome STAT5‐induced leukemic proliferation in AML treatment by inducing mitochondrial impairment.
Adipose‐derived stem cells: An appropriate selection for osteogenic differentiation
Authors: Shafaei Hajar , Kalarestaghi Hossein 
Published: April 2020

AbstractMesenchymal stem cells (MSCs) are a major component of various forms of tissue engineering. MSCs have self‐renewal and multidifferential potential. Osteogenic differentiation of MSCs is an area of attention in bone regeneration. One form of MSCs are adipose‐derived stem cells (ASCs), which can be simply harvested and differentiated into several cell lineages, such as chondrocytes, adipocytes, or osteoblasts. Due to special properties, ASCs are frequently used in vitro and in vivo bone regeneration. Identifying factors involved in osteogenic differentiation of ASCs is important for better understanding the mechanism of osteogenic differentiation. Different methods are used to stimulate osteogenesis of ASCs in literature, including common osteogenic media, growth factors, hormones, hypoxia, mechanical and chemical stimuli, genetic modification, and nanotechnology. This review article provides an overview describing the isolation procedure, characterization, properties, current methods for osteogenic differentiation of ASCs, and their basic biological mechanism.
Glucose, glycolysis, and neurodegenerative diseases
Authors: Tang Bor Luen 
Published: April 2020

AbstractProlonged survival of a typical postmitotic neuron hinges on a balance between multiple processes, among these are a sustenance of ATP production and protection against reactive oxygen species. In neuropathological conditions, mitochondrial defects often lead to both a drop in ATP levels, as well as increase reactive oxygen species production from inefficient electron transport processes and NADPH‐oxidases activities. The former often resulted in the phenomenon of compensatory aerobic glycolysis. The latter stretches the capacity of the cell's redox buffering capacity, and may lead to damages of key enzymes involved in energy metabolism. Several recent reports have indicated that enhancing glucose availability and uptake, as well as increasing glycolytic flux via pharmacological or genetic manipulation of glycolytic enzymes, could be protective in animal models of several major neurodegenerative diseases, including Parkinson's disease, Huntington's disease, and Amyotrophic lateral sclerosis. Activation of canonical Wnt signaling, which improves disease symptoms in mouse models of Alzheimer's disease also appears to work via an elevation of glycolytic enzymes and enhance glucose metabolism. Here, I discuss these findings and the possible underlying mechanisms of how an increase in glucose uptake and glycolysis could be neuroprotective. Increased glycolytic production of ATP would help alleviate energy deficiency, and ATP's hydrotropic effect may enhance solubility and clearance of toxic aggregates prevalent in many neurodegenerative diseases. Furthermore, channeling of glucose into the Pentose Phosphate Pathway would increase the redox buffering capacity of the cell.
Runoff water quantity and quality data from native tallgrass prairie and crop–livestock systems in Oklahoma between 1977 and 1999
Authors: Nelson Amanda M. , Moriasi Daniel N. , Fortuna Ann‐Marie , Steiner Jean L. , Starks Patrick J. , Northup Brian , Garbrecht Jurgen 
Published: April 2020

AbstractErosion and sedimentation pose serious threats to soil and water quality worldwide, including in the U.S. southern Great Plains. To better understand these processes in agricultural landscapes, eight 1.6‐ha watersheds were established and instrumented in 1976 at the USDA‐ARS Grazinglands Research Laboratory, ∼50 km west of Oklahoma City near El Reno, OK, to measure precipitation and surface runoff quantity and quality. Prior to construction, all watersheds were in native grass, primarily big bluestem (Andropogon gerardiiVitman.), little bluestem [Schizachyrium scoparium(Michx.) Nash], and Indiangrass [Sorghastrum nutans(L.) Nash]; afterwards, four of the eight watersheds were cropped initially into winter wheat (Triticum aestivumL.) (two conventionally tilled and two minimally or no‐till). Although there have been many peer‐reviewed papers from the Water Resources and Erosion (WRE) watersheds, none included all the datasets collected during the period 1977–1999. The objectives of this paper were (a) to present and discuss all archived historical data, including methods of collection and analysis, (b) to provide summary analyses of the variability in each dataset, and (c) to provide details about how to access these datasets. These datasets are valuable resources to improve modeling in relation to land use and management changes, climate variability, and other environmental factors and may be useful in developing strategies to mitigate environmental impacts of agricultural systems. They are available athttps://doi.org/10.15482/USDA.ADC/1518421.
On the maximum number of copies of H in graphs with given size and order
Authors: Gerbner Dániel , Nagy Dániel T. , Patkós Balázs , Vizer Máté 
Published: April 2020

AbstractWe study the maximum number of copies of a graph in graphs with a given number of vertices and edges. We show that for any fixed graph is asymptotically realized by the quasi‐clique provided that the edge density is sufficiently large. We also investigate a variant of this problem, when the host graph is bipartite.
Synthesis of novel heterocyclic compounds containing pyrimidine nucleus using the Biginelli reaction: Antiproliferative activity and docking studies
Authors: Çağlar Yavuz Sevtap , Akkoç Senem , Türkmenoğlu Burçin , Sarıpınar Emin 
Published: April 2020

AbstractIn a single reaction step, pyrimidine derivatives (4a‐p) were synthesized from the triple reaction of aromatic aldehydes (1), ethyl cyanoacetate (2), and some guanyl hydrazone derivatives (3a‐n). These compounds were tested as in vitro against two types of cancerous cell lines, namely, a human colon cancerous cell line (DLD‐1) and a human breast cancerous cell line (MDA‐MB‐231). According to the obtained results, nearly all the compounds have cytotoxic activity in the tested cell lines. Especially, the compounds 4j, 4k, and 4n had a significant effect against DLD‐1. Furthermore, compounds 4g, 4m, and 4o exhibited lower IC50 values compared to other synthesized compounds against MDA‐MB‐231. We hope that these compounds can be improved as anticancer agents in the future. Molecular docking was performed according to both topoisomerase I and N‐acetyltransferase 1 proteins to examine theoretically the binding mode and site of pyrimidine compounds having the best activity.
A Self‐Determination Approach to Understanding Individual Values as an Interaction Condition on Employees' Innovative Work Behavior in the High‐Tech Industry
Authors: Wang Zheni , Gao Mengchan , Panaccio Alexandra 
Published: April 2020

AbstractDrawing on self‐determination theory (SDT; E.L. Deci & R.M. Ryan, 1985, 2000) and using corporate samples (N = 284; 63% Male; Mean Age = 34,SD = 6.09) from high‐tech firms in China, this empirical study explored the path model from satisfaction of the basic psychological needs for autonomy, competence, and relatedness, through autonomous motivation to employees' innovative work behavior (“IWB”; J. De Jong & D. Den Hartog, 2010). It also simultaneously examined the interactions between autonomous work motivation and individual values (collectivism, H.C. Triandis (1995); uncertainty avoidance, R. J. House, P. J. Hanges, M. Javidan, P. W. Dorfman, & V. Gupta (2004); and power distance, J. Farh, R.D. Hackett, & J. Liang (2007)) in the conditioned path model. The regression results obtained from conditional PROCESS analysis (A.F. Hayes, 2013, 2018) suggest that basic psychological needs satisfaction is positively related to employees' IWB via autonomous motivation, but that these indirect effects were weaker when employees' power distance value orientation was high. Implications, limitations, and future research directions are discussed.
Treadmill exercise facilitated rotator cuff healing is coupled with regulating periphery neuropeptides expression in a murine model
Authors: Zhang Tao , Chen Yang , Chen Can , Li Shengcan , Xiao Han , Wang Linfeng , Hu Jianzhong , Lu Hongbin 
Published: April 2020

AbstractPostoperative exercise has been found able to accelerate bone‐tendon (B‐T) healing. In this study, we systematically compared tendon‐to‐bone healing in mice subjected to postoperative treadmill exercise and free cage recovery in a murine rotator cuff repair model. Specifically, C57BL/6 mice underwent unilateral supraspinatus tendon (SST) detachment and repair were randomly allocated into treadmill group and control group. Treadmill group received daily treadmill running initiated from postoperative day 7 while the control group was allowed free cage activity. Mice were euthanized at postoperative 4 and 8 weeks for synchrotron radiation micro‐computed tomography (SR‐μCT), histology and biomechanical tests to investigate the effect of treadmill running on B‐T healing. The results indicated that treadmill running initiated at day 7 postoperatively was able to accelerate B‐T healing, as evidenced by better tendon‐to‐bone maturation and increased mechanical property. Recent studies show that peripheral neuropeptides are closely associated with musculoskeletal tissue repair. We furtherly conducted quantitative reverse transcription‐polymerase chain reaction and immunofluorescence staining to investigate the temporal‐spatial expression of calcitonin gene‐related peptide (CGRP), substance P (SP), and peripheral neuropeptide Y (NPY) to verify whether they are related to rotator cuff healing. Our results show increased expression of CGRP, SP, and NPY at the healing site under the effect of mechanical stimulation. In conclusion, delayed postoperative exercise with moderate strength appears to accelerate the early phase of B‐T healing, a process that may prove to be linked to increased expression of periphery neuropeptides known to play a role in tissue healing.
Comparative Study on the Syntheses and Properties of Three N‐lauroyl Aromatic Amino Acid Surfactants
Authors: Yang Jian , Zhou Yawen , Yang Bo , Huang Luyang , Liu Changyao , Han Fu , Xu Baocai 
Published: April 2020

AbstractThree N‐lauroyl aromatic amino acid surfactants, namely, sodium N‐lauroyl phenylalaninate (SLP), sodium N‐lauroyl tyrosinate, and sodium N‐lauroyl tryptophanate (SLTr), with aromatic group structures were synthesized via methyl ester method. Mass spectrometry, Fourier transform infrared spectroscopy, and nuclear magnetic resonance were applied for the structural representation of products. A comparative study of the three surfactants in terms of surface, foam, and emulsifying properties and decontamination performance was conducted. Results demonstrated that aromatic structure significantly influenced the properties of the three surfactants. SLTr had the best surface performance and foam property, whereas SLP had the best emulsifying property. All three surfactants exhibited strong decontamination effects on protein‐soiled swatches.
Preparative separation of isoquinoline alkaloids from Corydalis impatiens using middle chromatogram isolated gel column coupled with positively charged reversed‐phase liquid chromatography
Authors: Wang Qi , Chen Wenjie , Wang Qilan , Tao Yanduo , Yu Ruitao , Pan Guoqing , Dang Jun 
Published: April 2020

AbstractPositively charged reversed‐phase liquid chromatography was employed for the efficient preparative separation of isoquinoline alkaloids from Corydalis impatiens. Ten commercially available columns were compared for isoquinoline alkaloids analysis. While tailing, overloading, lower resolution, and buffer salts limited the application in purification of isoquinoline compounds of many of these columns, one positively charged reversed‐phase C18 column (XCharge C18) overcame these drawbacks, allowing for favorable separation resolution, even when loading isoquinoline compounds on a larger, preparative scale. The general separation process is as follows. First, isoquinoline alkaloids are enriched with Corydalis impatiens extract via a middle chromatogram isolated gel column. After column selection, separation is performed on an XCharge C18 analytical column, from which two evident chromatographic peaks are readily obtained. Finally, two isoquinoline alkaloids (protopine and corydamine) are selectively purified on the XCharge C18 preparative column. These results demonstrate that a middle chromatogram isolated gel column coupled with positively charged reversed‐phase liquid chromatography is effective for the preparative separation of isoquinoline alkaloids from Corydalis impatiens.
Palladium‐Catalyzed Polycondensation for the Synthesis of Poly(Aryl)Sulfides
Authors: Hou Peng , Oechsle Peter , Kuckling Dirk , Paradies Jan 
Published: April 2020

AbstractThe palladium‐catalyzed CS cross coupling reaction is investigated as a novel efficient tool for the synthesis of poly(phenyl)sulfide derivatives. The reaction proceeds through the polycondensation of dibromo arenes with a H2S‐surrogate to yield poly(aryl)sulfides. The reaction is generalized by the synthesis of so‐far‐unprecedented poly(2,5‐thiophene)sulfide. Number average molecular weights (Msss) of up to 3780 and 1770 g mol−1 for poly(phenyl)sulfide and poly(thiophene)sulfide are achieved with degrees of polymerization (DPn) of 10 and 7, respectively. A mechanism for the new polycondensation reaction is suggested.