bluuebunny/crossref_metadata_2025_split huggingface dataset:
Appendicular skeletal muscle mass to truncal fat mass ratio as a new index for osteosarcopenic obesity syndrome in older adults: emphasizing the role of central obesity
Authors: Dwipa Lazuardhi , Widiastuti Rini , Rakhimullah Alif Bagus , Maharsidi Marcellinus , Pratiwi Yuni Susanti , Apandi Muhamad 
Published: February 2020

Abstract
Background The relationship between obesity and low bone mineral density (BMD) in older adults is still unclear. Most of the previous study did not account the factor of sarcopenia which is the progressive loss of skeletal muscle mass due to aging, and distribution of fat in obesity. Thus, this study was aimed to explore the correlation between appendicular skeletal muscle mass (ASMM), total fat mass (FM), and truncal fat mass (TrFM) as well as indexes (ASMM/FM and ASMM/TrFM ratio) with BMD in older adults.Methods This was an analytic cross-sectional study. Dual x-ray absorptiometry (DXA) and bioelectric impedance analysis (BIA) were used to assess BMD and body composition, respectively. Appendicular Skeletal Muscle Mass (ASMM) were used in the analysis to reflect sarcopenia, Fat Mass (FM) and Trunkal Fat Mass (TrFM) were used to reflect general and central obesity, respectively. All data were obtained from medical records of Geriatric Clinic of Hasan Sadikin General Hospital Bandung Indonesia from January 2014 to December 2018. The correlation between body compositions variable with BMD were analyzed using Spearman’s test. We also conducted a comparison analysis of body composition variables between low and normal BMD using Mann-Whitney test. Results A total of 112 subjects were enrolled in the study. ASMM and TrFM were positive (rs=0.517, p<0.001) and negative (rS=-0.22, p=0.02) correlated with BMD, respectively. FM were not correlated with BMD, rS=-0.113 (p=0.234). As indexes, ASMM/FM and ASMM/TrFM had positive correlation with BMD, rS=0.277 (p<0.001), and rS=0.391 (p<0.001), respectively. The ASMM, TrFM, and ASMM/TrFM ratio between normal and low BMD also significantly different (p<0.001), meanwhile FM were not (p=0.204).Conclusion ASMM and TrFM have a positive and negative correlation with BMD, respectively. ASMM/TrFM ratio as new sarcopenia-central obesity index has a positive correlation with BMD.
Impact of physical activity levels on cardiometabolic parameters and autonomic modulation in elderly: a cross-sectional study
Authors: Viana Ariane , Bugliani Neide , Sartori Michelle , Silva Camila Martins da , Sanches Iris Callado , Velardi Marília , Miranda Maria Luiza de Jesus , Angelis Katia De 
Published: February 2020

Abstract
Background: By 2030, approximately 20% of the population worldwide will be 65 years of age or older. In this age group, cardiovascular diseases (CVD) will result in 40% of all deaths. Heart rate variability (HRV) appears to be reduced under stress conditions and in many chronic diseases as cardiovascular diseases that appear to be associated with autonomic changes that usually include decreased parasympathetic activation and/or increased sympathetic modulation. Thus, aging is very important for altering neurohumoral mechanisms that control the cardiovascular system. Objective: To analyze the influence of level of physical activity in the HRV in elderly people. Methods: Cross-sectional analytical study which included 49 subjects (60 – 86 years), which answered the Baecke questionnaire. Results: The less physically active group had lower levels of plasma HDL (42±2.43mg/dL vs 52±3.8mg/dL) and higher systolic BP (131±117mmHg vs 117±4.07mmHg) when compared to the most active group. The more physically active group showed higher PI of variance (1849.6±387.7ms²) when compared to the moderate (892.1±141.9 ms²) and the less physically active groups (972.3±285.2 ms²). Moreover, the most physically active group presented lower LF/HF balance (1.6±0.2) when compared to the moderate (2.6±0.3) and the less active groups (2.5±0.2). Discussion: The results demonstrated the importance of physical activity to promote hemodynamic, autonomic and lipid profile improvement, suggesting a lower risk of developing or progressing the disease in the elderly.
Comparison of the diagnostic efficacy between transrectal prostate biopsy and transperineal cognitive fusion biopsy
Authors: Zhang Zhilei , Qin Fei , Jiao Wei , Yang Xuecheng , Li Yujun , Jiang Yanxia , Hao Dapeng , Li Jie , Zhou Ruizhi , Zhang Mingxin , Niu Haitao 
Published: February 2020

Abstract
Background: This study aimed to compare the detection efficacy of transrectal ultrasound -guided systematic prostate biopsy (TR-SB) and transperineal cognitive fusion targeted +systematic biopsy (TP-SB+COG-TB) in patients with suspected prostate cancer (PCa). In addition, the relative clinical characteristics of PCa were evaluated. Methods: 385 patients were enrolled in this study, who underwent transrectal (n=275) or transperineal (n = 110) cognitive fusion biopsy. Relative factors of PCa including age, prostate volume and PSA level were collected for multivariable analysis. The cancer detection rates were compared, and logistic regression was used to assess the impact of patient characteristics on PCa detection Results: For all patients, the overall detection rates of TR-SB and TP-SB+COG-TB were 121/275(40%) and 67/110（60.91%）, respectively. TP-SB+COG-TB detected a higher rate of PCa (P =0.003) and more clinically significant prostate cancers (csPCa) (P=0.001) than TR-SB. Logistic regression analyses revealed that age、TPSA（total prostate specific antigen）and PV were related to the detection rate of PCa (P≤0.05). Conclusion ： TP-SB+COG-TB could find more clinically significant PCa than TR-SB. Due to the high detection rate at certain ages, PSA levels and PV, patents’ clinical characteristics should be considered in biopsy.
Case report: a case of primary pulmonary glomus tumor
Authors: Deng shuangshuang , Huang Jianhao , Yin Qi , Gao Jinli , Wang Feilong , Lu Jingjing , Guo Zhongliang 
Published: February 2020

Abstract
Background Glomus tumors, as extremely rare tumors of the lung, since their rarity and the variety of the clinical symptoms, tend to be misdiagnosed. The location of these tumors as well as their early diagnosis is a pivot for the prognosis of the disease. Case presentation We report a case of old patient whose final diagnosis was primary pulmonary glomus tumor. She initially was found a nodule in the right lung, which was enlarged during follow-up and finally confirmed after surgery. Postoperative pathology was considered as a primary pulmonary glomus tumor. There has been no evidence of tumor recurrence or metastasis so far. Conclusion Primary pulmonary glomus tumor is rare. The clinical manifestations are related symptoms occupying lesions in the lung. The diagnosis of the tumor depends on histopathology and immunohistochemistry. Surgical resection is considered as the most eﬀective treatment for this condition.
Early Experience of the World’s First Single-Room Gantry Mounted Active Scanning Proton Therapy System at an Integrated Cancer Center
Authors: Forsthoefel Matthew , Ballew Elizabeth , Unger Keith R. , Ahn Peter H. , Rudra Sonali , Pang Dalong , Collins Sean P. , Dritschilo Anatoly , Harter K. William , Paudel Nitika , Collins Brian T. , Lischalk Jonathan W. 
Published: February 2020

Abstract
Introduction Review the early experience with a single-room gantry mounted active scanning proton therapy system implemented in the modern era. Materials and Methods All patients treated with proton beam radiotherapy (PBT) were enrolled in an institutional review board-approved patient registry. Proton beam radiotherapy was delivered with a 250 MeV gantry mounted synchrocyclotron in a single-room integrated facility within the pre-existing cancer center. Demographic data, cancer diagnoses, treatment technique, and geographic patterns were obtained for all patients. Treatment plans were evaluated for mixed modality therapy. Insurance approval data was collected for all patients treated with PBT. Results A total of 132 patients were treated with PBT between March 2018 and June 2019. The most common oncologic subsites treated included the central nervous system (22%), gastrointestinal tract (20%), and genitourinary tract (20%). The most common histologies treated included prostate adenocarcinoma (19%), non-small cell lung cancer (10%), primary CNS gliomas (8%), and esophageal cancer (8%). Rationale for PBT treatment included limitation of dose to adjacent critical organs at risk (67%), reirradiation (19%), and patient comorbidities (11%). Patients received at least one x-ray fraction delivered as prescribed (36%) or less commonly due to unplanned machine downtime (34%). Concurrent systemic therapy was administered to 57 patients (43%). Twenty-six patients (20%) were initially denied insurance coverage and required peer-to-peers (65%), written appeals (12%), secondary insurance approval (12%), and comparison x-ray to proton plans (8%) for subsequent approval. Proton beam radiotherapy approval required a median of 17 days from insurance submission. Conclusion Incorporation of PBT into our existing cancer center allowed for multidisciplinary oncologic treatment of a diverse population of patients. Insurance coverage for PBT presents as a significant hurdle and improvements are needed to provide more timely access to necessary oncologic care.
Liver X receptor inhibits the growth of hepatocellular carcinoma cells via regulating HULC/miR-134-5p/FOXM1 axis
Authors: Zhang Yan , He Jintao , Yang Teng , He Wenhui , Jiang Shan , Zhong Dan , Xu Zhizhen , Wei Quanfang , Shi Chunmong 
Published: February 2020

Abstract
Background Highly upregulated in liver cancer (HULC), the specifically overexpressed long non-coding RNA (lncRNA) in human hepatocellular carcinoma (HCC), can promote the growth and metastasis of HCC cells. Therefore, it will be benefit to HCC treatment by effectively downregulating HULC. Liver X receptor (LXR), a member of nuclear receptor superfamily, exerts anti-tumor effects on various human malignancies including HCC. However, it is unclear whether the anti-HCC function of LXR is involved in the regulation of HULC. Methods Quantitative real-time PCR and Western blot were used to separately examine RNA and protein levels in HCC cells. Cell counting kit-8 assay was used to detect the growth of HCC cells in vitro . Dual-luciferase reporter assays were performed to analyze the regulation of forkhead box M1 (FOXM1) by miR-134-5p and the regulation of miR-134-5p by HULC. Xenograft models were engaged to evaluate the growth of HCC cells in vivo . Results In this study, we found that activation of LXR could inhibit the growth of HCC cells by downregulating HULC. Mechanistically, LXR decreased HULC via suppressing its gene promoter activity. Moreover, HULC and FOXM1 were highly expressed while miR-134-5p was lowly expressed in HCC tissues, and the level of HULC was positively correlated with that of FOXM1 while negatively correlated with that of miR-134-5p. Additionally, miR-134-5p downregulated FOXM1 by targeting 3′-untranslated region (UTR) of its mRNA, and HULC upregulated FOXM1 and its downstream target molecule cyclin D1 through sequestrating miR-134-5p. Furthermore, activation of LXR increased miR-134-5p while decreased FOXM1 by reducing HULC in HCC cells. The in vivo experiments showed that activation of LXR repressed the growth of HCC xenografts, and decreased HULC, FOXM1 and cyclin D1 while increased miR-134-5p in the xenografts. Conclusions Our results for the first time reveal that LXR can inhibit the growth of HCC cells by regulating HULC/miR-134-5p/FOXM1 axis. The novel pathway LXR/HULC/miR-134-5p/FOXM1 may serve as a promising target in HCC treatment.
Identification of reliable reference genes for gene expression studies in maternal reproductive tissues and fetal tissues of pregnant cows
Authors: Cheng Lei , Yu Jie , Hu Xiuzhong , Xiang Min , Xia Yu , Tao Bifei , Du Xiaoyong , Wang Dingfa , Zhao Shuhong , Chen Hongbo 
Published: February 2020

Abstract
Background: The relationship between the conceptus and the maternal uterine environment is crucial for the successful establishment and maintenance of pregnancy in cattle. Gene expression analysis of the conceptus and maternal reproductive tissues is a favorable method to assess the embryonic maternal interaction. The reliability of the commonly used method reverse transcription-quantitative polymerase chain reaction (RT-qPCR) depends on proper normalization to stable reference genes (RGs). The objective of this study was to determine the expression stability of ten potential RGs (SUZ12, CNOT11, ACTB, RPL19, RPS9, GAPDH, TBP, HPRT1, SDHA and PPIA) in maternal reproductive tissues and fetal tissues, and to analyze the effect of RG selection on the calculation of the relative expression of target genes. Results: The expression stability of ten potential RGs was analyzed in eight different tissues (caruncular endometrium, intercaruncular endometrium, corpus luteum, ovary, oviduct, mammary gland, embryonic disc and trophoblast) from three pregnant dairy cows. Three programs—GeNorm, NormFinder and Bestkeeper—were used to identify the best RGs. According to all three programs, the most stable RG was CNOT11, whereas the least stable RGs were GAPDH and HPRT1. GeNorm analysis showed that a combination of five RGs (SDHA, PPIA, CNOT11, RPS9 and RPL19) was necessary for appropriate data normalization. However, NormFinder analysis indicated that the combination of CNOT11 and PPIA was the most suitable. When target genes were normalized to these RGs, the relative expression of the Radical S-adenosyl methionine domain containing 2 gene was not affected by the choice of RGs, whereas a large difference was observed in the expression profile of the Nuclear erythroid2-related factor 2 gene between the most stable RGs and least stable RGs. Conclusions: The results indicate that careful selection of RGs is crucial under different conditions, especially for target genes with relatively small fold changes. Furthermore, the results provide useful information for the selection of RGs for evaluating genes affecting bovine reproduction.
Fenofibrate Ameliorates Insulin Resistance of Lipoprotein Lipase Knockout Heterozygous Mice
Authors: Li Yangxue , Han Tingting , Zheng Shuang , Ren Xingxing , Hu Yaomin 
Published: February 2020

Abstract
Background The benefits of fenofibrate (FB), a peroxisome proliferator-activated receptor-a agonist, against hyperlipidemia have been established. We investigated the effect of fenofibrate on insulin resistance of lipoprotein lipase knockout heterozygous (LPL+/-) mice, which represent inherited hypertriglyceridemia and impaired glucose tolerance. Methods Male LPL+/- mice were treated with FB (50 mg/kg, once daily) via gavage for 8 weeks. Plasma lipid, glucose tolerance test, systemic insulin sensitivity, insulin signaling of tissues, genes and proteins related to endoplasmic reticulum (ER) stress and oxidative stress were analyzed. Results Body weight of 40-week LPL+/- with FB were reduced by 30.3% (P<0.05), while the differences of 16- and 28-week LPL+/- with FB were not significant (P>0.05). FB improved the lipid profile of both 28 and 40-week LPL+/- (P<0.001 for both), while that of 16-week LPL+/- mice with FB was unaltered (P>0.05). Glucose tolerance of 40-week LPL+/- were improved by FB (P<0.05), while that of 16- and 28-week LPL+/- with FB kept unaltered (P>0.05). Fasting insulin of 40-week LPL +/- were improved by FB (P<0.05), thus HOMA-IR of 40-week LPL+/- was declined (P<0.05). HOMA-IR of 16- and 28-week LPL+/- with FB had no change. Insulin-stimulated phosphorylated Akt (Ser473) in liver and skeletal muscle of 28-week LPL+/- was enhanced by FB (P < 0.001 and P<0.05 respectively). ER stress biomarkers were detected decreased in liver of 16- to 40-week LPL+/- with FB whereas that in muscle of LPL+/- with FB unchanged. Reduced reactive oxygen species (ROS) levels and augmented mRNA expression of superoxide dismutase (SOD) and catalase (CAT) in skeletal muscle of 28- and 40-week LPL+/- mice with FB were observed. There was no significance on ROS levels and mRNA of SOD and CAT in liver between LPL+/- mice with and without FB. Conclusions Fenofibrate improved lipid profile, glucose tolerance, systemic and tissue-specific insulin resistance of LPL knockout heterozygous mice. This may be associated with alleviated endoplasmic reticulum stress in liver and reduced oxidative stress in muscle.
Is Ankylosing Spondylitis a single disease? A clinical and genetic perspective
Authors: Laza Imanol Martín , Hervella Montserrat , Madrigal Juan Maria Blanco , Galíndez Eva , Rivera Natalia Andrea , Vivar Maria Luz Garcia , de-la-Rúa Concepción 
Published: February 2020

Abstract
Background:The aim of the present study was to evaluate whether ankylosing spondylitis (AS) and non-radiographic axial spondyloartritis (nr-axSpA) are subsets of a single disease from a genetic perspective. Methods:We analyzed from a clinical and genetic perspective 62 patients from the University Hospital of Basurto (Bilbao, Spain) diagnosed with axial spondyloarthritis. Forty three SNPs previously associated with spondyloarthritis (SpA) were selected. The DNA samples were genotyped through SNP Type Assay, using the BioMark HD platform of Fluidigm. Results:Regarding clinical characteristic we found statistically significant differences between the patients with AS and nr-axSpA in the age at diagnosis, the disease duration, the presence of syndesmophytes and the BASMI index. In relation to genetic markers, we only found statistically significant differences in HLA-B27 allele frequency between the two groups. Regarding the frequencies of the SNPs, no statistically significant differences were found between the two groups. Despite the high genetic heterogeneity observed among the patients, it is worth highlighting that some of the most important risk SNPs associated with AS, located in genes (ERAP1, ERAP2, IL-23R, GPR25) and intergenic region (2p15), appeared at high frequencies in all the patients.Conclusion:We have observed that AS and nr-axSpA have a common genetic background associated with the pathogenic development of these diseases; therefore, we suggest that the two entities constitute two different expressions of the same disease. Among the genetic factors, the present study shows the importance of genes involved in the pathogenesis of AS, such as HLA-B27, ERAP1, ERAP2, IL-23R, GPR25 and intergenic region 2p15, whose role may influence the onset, development and severity of the disease. However, the pathogenesis of SpA is very complex, indicating the involvement of environmental factors (smoking and obesity), in the triggering of the disease, so that patients with different genotypes would have the same pathogenic phenotype.
Cristianismo ou conservadorismo? O caso do movimento anti-aborto no Brasil
Authors: Rocha Camila 
Published: February 2020

O objetivo deste artigo é interpretar atuação do movimento anti-aborto no Brasil ressaltando seu caráter conservador em detrimento de sua caracterização como um movimento cristão ou anti-secular. O movimento anti-aborto teria surgido após a redemocratização como um contra-movimento em oposição ao avanço do movimento feminista, passando a realizar posteriormente inúmeras disputas relacionadas a pautas feministas, LGBT, e relacionadas à defesa de direitos humanos no legislativo, no judiciário e em protestos e demonstrações públicas. O argumento principal desenvolvido neste artigo, a partir da revisão da literatura especializada e reunião de informações relacionadas aos atores políticos envolvidos no embate em torno da questão do aborto, é de que a atuação como movimento social, e não apenas com base na atuação de padres, missionários e bispos, fez com que fosse possível para os ativistas anti-aborto aglutinar em torno de um mesmo discurso pessoas conservadoras que simplesmente se identificassem com suas pautas independentemente de sua confissão religiosa, e para tanto mobilizam fundamentalmente argumentos seculares.
Modulatory Nano/Micro Effects of Diabetes Development on Pharmacology of Primary and Secondary Bile Acids Concentrations
Authors: Mooranian Armin , Zamani Nassim , Takechi Ryu , Luna Giuseppe , Mikov Momir , Goločorbin-Kon Svetlana , Kovacevic Bozica , Arfuso Frank , Al-Salami Hani 
Published: February 2020

Background:
Recent studies have suggested that hyperglycaemia influences the bile acid
profile and concentrations of secondary bile acids in the gut.

Introduction:
This study aimed to measure changes in the bile acid profile in the gut, tissues, and faeces
in type 1 Diabetes (T1D) and Type 2 Diabetes (T2D).

Method:
T1D and T2D were established in a mouse model. Twenty-one seven-weeks old balb/c mice
were randomly divided into three equal groups, healthy, T1D and T2D. Blood, tissue, urine and faeces
samples were collected for bile acid measurements.

Results:
Compared with healthy mice, T1D and T2D mice showed lower levels of the primary bile
acid, chenodeoxycholic acid, in the plasma, intestine, and brain, and higher levels of the secondary bile
acid, lithocholic acid, in the plasma and pancreas. Levels of the bile acid ursodeoxycholic acid were
undetected in healthy mice but were found to be elevated in T1D and T2D mice.

Conclusion:
Bile acid profiles in other organs were variably influenced by T1D and T2D development,
which suggests similarity in effects of T1D and T2D on the bile acid profile, but these effects were not
always consistent among all organs, possibly since feedback mechanisms controlling enterohepatic recirculation
and bile acid profiles and biotransformation are different in T1D and T2D.
Mental Health Literacy: A Strategy for Global Adolescent Mental Health Promotion
Authors: Clauss-Ehlers Caroline S. , Carpio Maria Guevara , Weist Mark D. 
Published: February 2020

Background:
Adolescence is defined by key transitional elements which are considered
within a cross-cultural context. The importance of building mental health capacity for
adolescents in low- and middle-income countries (LMICs) as well as high-income countries
(HICs) is reviewed.

Objectives:
To review the developmental period of adolescence, global needs for mental
health promotion, the needs of LMICs while emphasizing building adolescent mental health
capacity, and the importance of efforts to promote mental health literacy.

Methods:
Mental health literacy (MHL) is presented as a strategy that can increase public
awareness regarding mental health issues among adolescents. Increased awareness through
an MHL framework is discussed as a way to build adolescent mental health capacity; with
this work ideally occurring through global communities of practice (COP), dialogue, collaboration,
and mutual support that aim to build innovation in systems of mental health promotion.

Results:
The authors review structural components in research, practice, and policy that seek
to build global adolescent mental health capacity, nested within COPs involving HICs and
LMICs working together to advance mental health promotion for children, adolescents, and
young people.

Conclusion:
The article concludes with a discussion of how the three structural components
(i.e., research, practice, and policy) can address gaps in the provision of global mental health
services for adolescents to meet adolescent mental health needs in LMICs and HICs. A
multi-sectoral approach emphasizing a global COP is presented as a way to scale up capacity
and maximize outcomes.