bluuebunny/crossref_metadata_2025_split huggingface dataset:
Neuroinflammatory mechanisms of post-traumatic epilepsy
Authors: Mukherjee Sanjib , Arisi Gabriel M. , Mims Kaley , Hollingsworth Gabriela , O’Neil Katherine , Shapiro Lee A. 
Published: June 2020

Abstract
Background
Traumatic brain injury (TBI) occurs in as many as 64–74 million people worldwide each year and often results in one or more post-traumatic syndromes, including depression, cognitive, emotional, and behavioral deficits. TBI can also increase seizure susceptibility, as well as increase the incidence of epilepsy, a phenomenon known as post-traumatic epilepsy (PTE). Injury type and severity appear to partially predict PTE susceptibility. However, a complete mechanistic understanding of risk factors for PTE is incomplete.

Main body
From the earliest days of modern neuroscience, to the present day, accumulating evidence supports a significant role for neuroinflammation in the post-traumatic epileptogenic progression. Notably, substantial evidence indicates a role for astrocytes, microglia, chemokines, and cytokines in PTE progression. Although each of these mechanistic components is discussed in separate sections, it is highly likely that it is the totality of cellular and neuroinflammatory interactions that ultimately contribute to the epileptogenic progression following TBI.

Conclusion
This comprehensive review focuses on the neuroinflammatory milieu and explores putative mechanisms involved in the epileptogenic progression from TBI to increased seizure-susceptibility and the development of PTE.
25-Hydroxycholesterol amplifies microglial IL-1β production in an apoE isoform-dependent manner
Authors: Wong Man Ying , Lewis Michael , Doherty James J. , Shi Yang , Cashikar Anil G. , Amelianchik Anna , Tymchuk Svitlana , Sullivan Patrick M. , Qian Mingxing , Covey Douglas F. , Petsko Gregory A. , Holtzman David M. , Paul Steven M. , Luo Wenjie 
Published: June 2020

Abstract
Background
Genome-wide association studies of Alzheimer’s disease (AD) have implicated pathways related to lipid homeostasis and innate immunity in AD pathophysiology. However, the exact cellular and chemical mediators of neuroinflammation in AD remain poorly understood. The oxysterol 25-hydroxycholesterol (25-HC) is an important immunomodulator produced by peripheral macrophages with wide-ranging effects on cell signaling and innate immunity. Cholesterol 25-hydroxylase (CH25H), the enzyme responsible for 25-HC production, has also been found to be one of the disease-associated microglial (DAM) genes that are upregulated in the brain of AD and AD transgenic mouse models.

Methods
We used real-time PCR and immunoblotting to examine CH25H expression in human AD brain tissue and in transgenic mouse brain tissue-bearing amyloid-β plaques or tau pathology. The innate immune response of primary mouse microglia under different treatment conditions or bearing different genetic backgrounds was analyzed using ELISA, western blotting, or immunocytochemistry.

Results
We found that CH25H expression is upregulated in human AD brain tissue and in transgenic mouse brain tissue-bearing amyloid-β plaques or tau pathology. Treatment with the toll-like receptor 4 (TLR4) agonist lipopolysaccharide (LPS) markedly upregulates CH25H expression in the mouse brain and stimulates CH25H expression and 25-HC secretion in mouse primary microglia. We found that LPS-induced microglial production of the pro-inflammatory cytokine IL-1β is markedly potentiated by 25-HC and attenuated by the deletion of CH25H. Microglia expressing apolipoprotein E4 (apoE4), a genetic risk factor for AD, produce greater amounts of 25-HC than apoE3-expressing microglia following treatment with LPS. Remarkably, 25-HC treatment results in a greater level of IL-1β secretion in LPS-activated apoE4-expressing microglia than in apoE2- or apoE3-expressing microglia. Blocking potassium efflux or inhibiting caspase-1 prevents 25-HC-potentiated IL-1β release in apoE4-expressing microglia, indicating the involvement of caspase-1 inflammasome activity.

Conclusion
25-HC may function as a microglial-secreted inflammatory mediator in the brain, promoting IL-1β-mediated neuroinflammation in an apoE isoform-dependent manner (E4>>E2/E3) and thus may be an important mediator of neuroinflammation in AD.
Contraceptive use and associated factors among women seeking induced abortion in Debre Marko’s town, Northwest Ethiopia: a cross-sectional study
Authors: Alemu Lebeza , Ambelie Yeshambel Agmus , Azage Muluken 
Published: June 2020

Abstract
Background
Contraceptive utilization is a practice that helps individuals or couples to avoid unwanted pregnancy. Even though there is the widespread availability of contraceptives, induced abortion remains an alarming public health problem in Ethiopia. Nationally, more than a third (35%) of women seeking an induced abortion service had a previous history of abortion. Therefore, this study aimed to assess the history of contraceptive use and identify associated factors among women seeking an induced abortion service in Debre Marko’s town, Ethiopia.

Methods
An institutional-based cross-sectional study was conducted from March 15 to May 15, 2019. The sample size was 416 and each health institution was proportionally allocated based on the previous 2 months of patient flow. Systematic random sampling was used to select the study participants. A structured questionnaire was used to collect the data. Data were entered by EPI-data and analyzed using SPSS version 23. Bivariate and multivariable logistic regression analyses were carried out. Model fitness was assured.

Results
The proportion of contraceptive use within the last 6 months before pregnancy was 41.3% among women seeking an induced abortion. Women who had good knowledge about contraceptives (AOR = 3.9; 95%CI: 2.36, 6.54), women who had a positive attitude about contraceptives (AOR=; 95%CI: 1.02, 2.56), women who had living children (AOR = 2.1; 95%CI; 1.04, 4.11), women who had frequent sexual practice (AOR = 2.5; 95% CI; 1.53, 4.21) and women discussed with their sexual partners about contraceptives (AOR = 1.9; 95%CI: 1.18, 3.18) were increase the odds of contraceptive use among women seeking an induced abortion.

Conclusion
Contraceptive use among women seeking an induced abortion was low despite the expected national goal of 55% contraceptive use in 2020. Having good knowledge and having a positive attitude on contraceptives, and having a discussion on contraceptives with sexual partner were increase the odds of contraceptive use. The intervention should focus on abortion seeking women to achieve their contraceptive needs and encourage sexual partner discussion about contraceptives to improve joint partner collective decision-making.
Effects of perinatal factors on sirtuin 3, 8-hydroxy-2′- deoxyguanosine, brain-derived neurotrophic factor and serotonin in cord blood and early breast milk: an observational study
Authors: Nyárády Kata , Turai Réka , Funke Simone , Györgyi Erzsébet , Makai Alexandra , Prémusz Viktória , Bódis József , Sulyok Endre 
Published: June 2020

AbstractBackgroundThe profile of sirtuin 3 (SIRT3), 8-hydroxy-2′-deoxyguanosine (8-OHdG), brain-derived neurotrophic factor (BDNF) and serotonin (5-HT) in cord blood and in early breast milk was studied and it was related to perinatal factors. 5-HT and BDNF signalling systems have been claimed to play a critical role in intrauterine development, postnatal adaptation and lactation. Since prematurity and Caesarean birth are frequently associated with inflammation and related oxidative stress, an attempt was made to reveal the adaptive changes of the protective SIRT3 and the complex interplay among these bioactive components in cord blood and early breast milk.MethodsThree groups each consisting of 30 mothers were included in the study: mothers who underwent spontaneous vaginal birth at term (group I), Caesarean section at term (group II) and preterm birth (group III). Venous cord blood and early breast milk samples were collected for measuring the biomarkers. SIRT3, 8-OHdG, BDNF and 5-HT levels were determined by using commercially available ELISA kits.ResultsIt was demonstrated that cord blood levels of SIRT3, BDNF and 5-HT were markedly reduced whereas those of 8-OHdG were significantly elevated after preterm birth when compared with birth at term. The Caesarean section was associated with a moderate decrease in BDNF and 5-HT, however, both SIRT3 and 8-OHdG remained unaffected. Breast milk levels of all biomarkers studied proved to be independent of their corresponding cord blood concentrations. In response to preterm birth breast milk SIRT3, 8-OHdG and 5-HT increased significantly, while a drastic fall occurred in BDNF. A significant positive relationship was found of 5-HT with SIRT3 and 8-OHdG irrespective of the gestational age and the mode of delivery.ConclusionsIt is suggested that the selected biomarkers in the breast milk mostly derive from local production by the mammary glands and 5-HT may have an essential role in the control of this process.
Impetus to change: a multi-site qualitative exploration of the national audit of dementia
Authors: Sykes Michael , Thomson Richard , Kolehmainen Niina , Allan Louise , Finch Tracy 
Published: June 2020

AbstractBackgroundNational audit is a key strategy used to improve care for patients with dementia. Audit and feedback has been shown to be effective, but with variation in how much it improves care. Both evidence and theory identify active ingredients associated with effectiveness of audit and feedback. It is unclear to what extent national audit is consistent with evidence- and theory-based audit and feedback best practice.MethodsWe explored how the national audit of dementia is undertaken in order to identify opportunities to enhance its impact upon the improvement of care for people with dementia. We undertook a multi-method qualitative exploration of the national audit of dementia at six hospitals within four diverse English National Health Service organisations. Inductive framework analysis of 32 semi-structured interviews, documentary analysis (n= 39) and 44 h of observations (n= 36) was undertaken. Findings were presented iteratively to a stakeholder group until a stable description of the audit and feedback process was produced.ResultsEach organisation invested considerable resources in the audit. The audit results were dependent upon the interpretation by case note reviewers who extracted the data. The national report was read by a small number of people in each organisation, who translated it into an internal report and action plan. The internal report was presented at specialty- and organisation-level committees. The internal report did not include information that was important to how committee members collectively decided whether and how to improve performance. Participants reported that the national audit findings may not reach clinicians who were not part of the specialty or organisation-level committees.ConclusionsThere is considerable organisational commitment to the national audit of dementia. We describe potential evidence- and theory-informed enhancements to the enactment of the audit to improve the local response to performance feedback in the national audit. The enhancements relate to the content and delivery of the feedback from the national audit provider, support for the clinicians leading the organisational response to the feedback, and the feedback provided within the organisation.
KAML: improving genomic prediction accuracy of complex traits using machine learning determined parameters
Authors: Yin Lilin , Zhang Haohao , Zhou Xiang , Yuan Xiaohui , Zhao Shuhong , Li Xinyun , Liu Xiaolei 
Published: June 2020

AbstractAdvances in high-throughput sequencing technologies have reduced the cost of genotyping dramatically and led to genomic prediction being widely used in animal and plant breeding, and increasingly in human genetics. Inspired by the efficient computing of linear mixed model and the accurate prediction of Bayesian methods, we propose a machine learning-based method incorporating cross-validation, multiple regression, grid search, and bisection algorithms named KAML that aims to combine the advantages of prediction accuracy with computing efficiency. KAML exhibits higher prediction accuracy than existing methods, and it is available athttps://github.com/YinLiLin/KAML.
Stakeholder perspectives on adaptive clinical trials: a scoping review
Authors: Madani Kia Tina , Marshall John C. , Murthy Srinivas 
Published: June 2020

Abstract
Background
Adaptive clinical trials (ACTs) represent an emerging approach to trial design where accumulating data are used to make decisions about future conduct. Adaptations can include comparisons of multiple dose tiers, response-adaptive randomization, sample size re-estimation, and efficacy/futility stopping rules. The objective of this scoping review is to assess stakeholder attitudes, perspectives, and understanding of adaptive trials.

Methods
We conducted a review of articles examining stakeholders encompassing the broad medical trial community’s perspectives of adaptive designs (ADs). A computerized search was conducted of four electronic databases with relevant search terms. Following screening of articles, the primary findings of each included article were coded for study design, population studied, purpose, and primary implications.

Results
Our team retrieved 167 peer-reviewed titles in total from the database search and 5 additional titles through searching web-based search engines for gray literature. Of those 172 titles, 152 were non-duplicate citations. Of these, 119 were not given full-text reviews, as their titles and abstracts indicated that they did not meet the inclusion criteria. Thirty-three articles were carefully examined for relevance, and of those, 18 were chosen to be part of the analysis; the other 15 were excluded, as they were not relevant upon closer inspection.
Perceived advantages to ADs included limiting ineffective treatments and efficiency in answering the research question; −perceived barriers included insufficient sample size for secondary outcomes, challenges of consent, potential for bias, risk of type 1 error, cost and time to adaptively design trials, unclear rationales for using Ads, and, most importantly, a lack of education regarding ADs among stakeholders within the clinical trial community. Perceptions among different types of stakeholders varied from sector to sector, with patient perspectives being noticeably absent from the literature.

Conclusion
There are diverse perceptions regarding ADs among stakeholders. Further training, guidelines, and toolkits on the proper use of ADs are needed at all levels to overcome many of these perceived barriers. While education for principal investigators is important, it is also crucial to educate other groups in the community, such as patients, as well as clinicians and staff involved in their daily implementation.
Unexpected high frequency of neurofibroma in the celiac ganglion of German cattle
Authors: Dammann Insa , Wemheuer Wiebke M. , Wrede Arne , Wemheuer Wilhelm E. , Campe Amely , Petschenka Jutta , Schulze-Sturm Ulf , Hahmann Uwe , Czerny Claus P. , Münster Pia , Brening Bertram , Kreienbrock Lothar , Herden Christiane , Schulz-Schaeffer Walter J. 
Published: June 2020

AbstractIn a study originally designed to find potential risk factors for bovine spongiform encephalopathy (BSE) we examined tissues from 403 Holstein Frisian cattle in total. These included 20 BSE cattle and their 236 birth- and feeding cohort animals plus 32 offspring, 103 age, breed and district-matched control cattle and further twelve cattle with neurological signs. In addition to the obex, we examined the celiac ganglion, cervical cranial ganglion, trigeminal ganglion and proximal ganglion of the vagus nerve using histological techniques. Unexpectedly, we found a high number of neurofibroma, a benign peripheral nerve sheath tumor consisting of Schwann cells, fibroblasts and perineural cells. The neurofibroma were present only in the celiac ganglion and found during histologic examination. With a frequency of 9.91% in BSE cattle and their cohorts (case animals) and 9.09% in the age, breed and district matched control animals there seems to be no correlation between the occurrence of BSE and neurofibroma. Benign peripheral nerve sheath tumors have been described more often in cattle than in other domestic animals. Usually, they are incidental macroscopic findings in the thoracic ganglia during meat inspection. To our knowledge, there are no previous systematic histologic studies including bovine celiac ganglia at all. The high incidence of celiac ganglia neurofibroma may play a role in the frequently occurring abomasal displacements in Holstein Frisian cattle as the tumors might cause a gastrointestinal motility disorder. At present a genetic predisposition for these neoplasms cannot be ruled out.
Improved two-stream model for human action recognition
Authors: Zhao Yuxuan , Man Ka Lok , Smith Jeremy , Siddique Kamran , Guan Sheng-Uei 
Published: June 2020

AbstractThis paper addresses the recognitions of human actions in videos. Human action recognition can be seen as the automatic labeling of a video according to the actions occurring in it. It has become one of the most challenging and attractive problems in the pattern recognition and video classification fields. The problem itself is difficult to solve by traditional video processing methods because of several challenges such as the background noise, sizes of subjects in different videos, and the speed of actions. Derived from the progress of deep learning methods, several directions are developed to recognize a human action from a video, such as the long-short-term memory (LSTM)-based model, two-stream convolutional neural network (CNN) model, and the convolutional 3D model.In this paper, we focus on the two-stream structure. The traditional two-stream CNN network solves the problem that CNNs do not have satisfactory performance on temporal features. By training a temporal stream, which uses the optical flow as the input, a CNN can have the ability to extract temporal features. However, the optical flow only contains limited temporal information because it only records the movements of pixels on thex-axis and they-axis. Therefore, we attempt to design and implement a new two-stream model by using an LSTM-based model in its spatial stream to extract both spatial and temporal features in RGB frames. In addition, we implement a DenseNet in the temporal stream to improve the recognition accuracy. This is in-contrast to traditional approaches which typically utilize the spatial stream for extracting only spatial features. The quantitative evaluation and experiments are conducted on the UCF-101 dataset, which is a well-developed public video dataset. For the temporal stream, we choose the optical flow of UCF-101. Images in the optical flow are provided by the Graz University of Technology. The experimental result shows that the proposed method outperforms the traditional two-stream CNN method with an accuracy of at least 3%. For both spatial and temporal streams, the proposed model also achieves higher recognition accuracies. In addition, compared with the state of the art methods, the new model can still have the best recognition performance.
The intrinsic plasticity of medial vestibular nucleus neurons during vestibular compensation—a systematic review and meta-analysis
Authors: Wijesinghe Rajiv , Camp Aaron 
Published: June 2020

Abstract
Background
Vestibular compensation is a homeostatic process that occurs in the central nervous system in response to peripheral vestibular dysfunction. Experimental studies in rodent models have suggested that unilateral peripheral vestibular lesions are correlated with an increase in the intrinsic excitability of central vestibular neurons. This process may be dependent on the intrinsic properties of the neurons themselves. We aimed to conduct a systematic review of the literature to survey the evidence for changes in intrinsic plasticity observed during the acute phase of vestibular compensation.

Methods
We systematically reviewed the literature regarding the electrophysiological effect of experimentally induced unilateral vestibular deafferentation (UVD) on the intrinsic membrane properties of medial vestibular nucleus neurons in animal models. We developed tools to assess the methodological quality (precision, validity and bias) of studies that met pre-determined inclusion and exclusion criteria. We extracted numerical data and performed a meta-analysis of specific quantitative data pooled from these studies.

Results
We identified 17 studies that satisfied the inclusion criteria. There is moderate quality evidence to suggest a statistically significant increase in the intrinsic excitability of medial vestibular nucleus neurons following unilateral vestibular deafferentation. Specifically, the spontaneous discharge rate increases by 4 spikes/s on average and the sensitivity to current stimuli increases.

Conclusion
Using this novel approach, we demonstrate that the methodology of systematic review and meta-analysis is a useful tool in the summation of data across experimental animal studies with similar aims.
Microstructure and long-term corrosion of archaeological iron alloy artefacts
Authors: Grevey A.-L. , Vignal V. , Krawiec H. , Ozga P. , Peche-Quilichini K. , Rivalan A. , Mazière F. 
Published: June 2020

Abstract
The smithing skills of craftsmen in the western Mediterranean during the early Iron Age can be determined by studying the microstructure of oxidised iron items. While some ghost structures have already been identified in dense corrosion products, their formation has not always been explained. Four objects from southern France were analysed using optical microscopy, X-ray diffraction, micro-Raman spectroscopy and field-emission scanning electron microscopy coupled with energy dispersive spectroscopy and electron backscatter diffraction. The role of microstructure, grain boundaries, cracks, species diffusion through oxides in soil corrosion and the initial microstructure are discussed. An additional parallel degradation mechanism (graphitisation) was experimentally revealed.
COVID-19 pandemic in sub-Saharan Africa: preparedness, response, and hidden potentials
Authors: Osseni Issideen Ayinla 
Published: June 2020

AbstractAfter the detection of coronavirus disease 2019 (COVID-19) first reported case in Nigeria, the virus has spread to all sub-Saharan Africa (SSA). Through different initiatives, SSA countries came together to create goal-driven taskforces to improve their responses against the virus. As COVID-19 raises major concerns over the scarcity of medical supplies, numbers of SSA countries adopted innovative solutions to fill in their shortage. This health crisis may come as an opportunity for SSA to demonstrate its pandemic readiness, responses, and reveal unknown potential.