bluuebunny/crossref_metadata_2025_split huggingface dataset:
Occupational benzene exposure and the risk of genetic damage: a systematic review and meta-analysis
Authors: Zhou Yanhua , Wang Kun , Wang Boshen , Pu Yuepu , Zhang Juan 
Published: May 2020

Abstract
Background To systematically evaluate the influence of benzene exposure on the genetic damage index of workers, and to explore the influence of low concentration benzene exposure on workers’ genetic damage index using 3.25mg/m3 as the boundary value, in order to provide a basis for improved prevention and control of the harm from benzene exposure to the occupational population. Methods We conducted a search of five databases, including Pub Med, Web of Science,China National Knowledge Infrastructure(CNKI), Wan Fang Data and Chongqing VIP, to identify relevant articles up to December 25, 2018. Two researchers independently extracted and carefully evaluated the data according to the inclusion and exclusion criteria of the literature. The imported articles were managed by Endnote X7, and the data were extracted and sorted by Excel 2013. We utilized Stata 12.0 software to perform the meta-analysis in the present study. Results A total of 68 eligible articles were finally included for the synthetic analyses. The meta-analysis results showed that occupational benzene exposure led to significantly increased Micronucleus (MN) frequency, Sister chromatid exchange (SCE) frequency, Chromosome aberration (CA) frequency, Olive Tail moment (OTM), Tail moment (TM), Tail length (TL), and Tail DNA% (T DNA%) compared to the control group (P < 0.05), and the pooled effect value estimates were 1.36, 0.98, 0.76, 1.06, 0.96, 1.78, and 1.42, respectively. Subsequent analysis of the effect of low concentration benzene exposure on genetic damage found significantly increased MN frequency increased compared with the control group (P < 0.05). Conclusions Occupational benzene exposure can affect multiple genetic damage indicators. Even at an exposure concentration lower than 3.25mg/m3, benzene exposure has genotoxicity. These data provide an important scientific basis for the further revision of occupational disease prevention strategies in China. At the same time, increased attention should be focused on the health monitoring of the occupational population exposed to benzene, and health management should be strengthened to improve the health of the occupational population.
Spatial distribution and determinant factors of Female Genital Mutilation among reproductive age women in Ethiopia, 2016; Based on Ethiopian National Demographic and Health Survey
Authors: Muluneh Atalay Goshu , Kassa Getahun Molla , Merid Mehari WoldeMariam 
Published: May 2020

Abstract
Background: Though condemned and considered as a crime by the countries government, Female Genital Mutilation (FGM) remains a common public health problem in Africa and Ethiopia as well. Every year, more than 3 million females undergo FGM and most of them are in Africa. Thus, this study was aimed to assess the spatial distribution and associated factors of female genital mutilation in Ethiopia based on the Ethiopian demographic and Health Survey 2016 data.Method: This is a secondary data analysis of Ethiopian Demographic and Health Survey (EDHS) 2016 data based on 7,163 women who were included for the FGM interview. The data were weighted using sampling weight as recommended by the program. The MS Excel and ArcGIS 10.3 software were used for data cleaning and spatial analysis respectively. Global and local level clustering was assessed. For the none spatial data and the determinant factors, data cleaning and analysis were done using STATA 14. Since the data has significant clustering with the Intraclass Correlation Coefficient [ICC=0.61(0.56, 0.65)], a multi-level mixed-effect logistic regression model was fitted. Variables with a P-value <0.25 in the bi-variable analysis were fitted in the multi-variable analysis. Finally, variables with p-value <0.05 with 95% CI of adjusted odds ratio were reported as a statistically significant determinant of FGM.Result: Female genital mutilation was spatially clustered (Global Moran’s I: 0.46, p<0.001). Significant hot spot clusters were found in Amhara, Oromia, Southern Nations Nationalities and Peoples (SNNP) regions. Mothers age >30 (AOR=2.41, 95% CI: 1.78,3.26) years, never in union (AOR=0.31, 95%CI: 0.22, 0.44), currently not working (AOR=0.71, 95%CI: 0.55, 0.92), women who considered FGM to be continued (AOR=2.86, 95%CI: 1.75, 4.68), not heard of FGM (AOR=0.22, 95%CI: 0.08,0.62), had no formal education (AOR=1.67, 95% CI: 1.03, 2.71), Muslim (AOR=3.90, 95%CI:2.5, 6.09) and protestant (AOR=1.76, 95%CI: 1.25, 2.97), and those who thought of FGM required by religion (AOR=1.99, 95%CI: 1.31,2.99) were found to be significant determinants of female genital mutilation.Conclusion: Female genital mutilation was spatially clustered with hotspot areas located:in Amhara, Oromia, and SNNP regions. Age of the mother, religion, occupation, educational level, marital status, information about Female genital mutilation, and intention about FGM to be stopped or continued were significant determinants of female genital mutilation
The Association of Hearing Impairment and Its Severity with Physical and Mental Health among Chinese Middle-aged and Older Adults
Authors: Ye Xin , Zhu Dawei , Chen Siyuan , He Ping 
Published: May 2020

Abstract
Background: Hearing loss is a common chronic condition which can be closely related with people’s health. However, current studies on this topic are quite limited in developing countries, and few with standardized audiometric measurement and multiple health outcomes. Therefore, we aimed to explore the association between hearing impairment and its severity with physical and mental health among Chinese middle-aged and older adults. Methods: We obtained data from two sources: (1) China Health and Retirement Longitudinal Study (CHARLS) 2011, 2013, and 2015, in which hearing impairment was measured by asking whether participants aged 45 years old had hearing problems; and (2) Hearing Survey 2019, the baseline survey of a randomized controlled trial conducted in Shandong Province of China, including 376 middle-aged and older participants. The severity of hearing impairment was identified by pure tone average of hearing thresholds at 0.5, 1, 2, and 4 kHz. Results: In CHARLS, 1248 (8.36%) participants suffered from hearing impairment at baseline, and hearing-impaired individuals were more likely to have chronic diseases, impaired activities of daily living (ADLs), impaired instrumental activities of daily living (IADLs) and depressive symptoms. For the 376 hearing-impaired participants in Hearing Survey 2019, 30.32%, 38.30% and 31.38% of them had moderate, severe and profound hearing impairment, respectively. As the severity of hearing impairment increased, individuals were likely to have impaired ADLs, impaired IADLs and depressive symptoms. Conclusions: Hearing impairment and its severity were closely related to multiple physical and mental health outcomes among Chinese middle-aged and older adults. Actions should be taken to prevent and treat hearing impairment, so as to improve people’s health and well-being.
Clinical assessment of three-dimensional printing assisted osteotomy guide plates in precise osteotomy of adult talipes equinovarus
Authors: Zhang Yuan-Wei , Xiong Liang-Yu , Huang Zu-Tai , Gao Wen-Cheng , Xiao Xin , Zhang Su-Li , Ni Wen-Yan , Deng Liang 
Published: May 2020

Abstract
The authors have withdrawn this preprint due to author disagreement.
Adipose Stem Cells from Type 2 Diabetic Mice Exhibit Therapeutic Potential in Wound Healing
Authors: Sun Yongfa , Song Lili , Zhang Yong , Wang Hongjun , Dong Xiao 
Published: May 2020

Abstract
BACKGROUND: Diabetic patients suffer from impaired wound healing. Mesenchymal stem cell (MSC) therapy represents a promising approach toward improving skin wound healing through release of soluble growth factors and cytokines that stimulate new vessel formation and modulate inflammation. Whether adipose-derived MSCs (ASCs) from type 2 diabetes donors are suitable for skin damage repair remains largely unknown. METHODS: In this study, we compared the phenotype and functionality of ASCs harvested from high fat diet (HFD) and streptozotocin (STZ)-induced T2D or control mice, and assessed their abilities to promote wound healing in an excisional wound splinting mouse model with T2D. RESULTS: T2D ASCs expressed similar cellular markers as control ASCs, but secreted less hepatocyte growth factor (HGF), vascular endothelial growth factor (VEGF), and transforming growth factor β (TGF-β). T2D ASCs were somewhat less effective in promoting healing of the wound, as manifested by slightly reduced re-epithelialization, cutaneous appendage regeneration, and collagen III deposition in wound tissues. In vitro, T2D ASCs promoted proliferation and migration of skin fibroblasts to a comparable extent as control ASCs via suppression of inflammation and macrophage infiltration. CONCLUSIONS: From these findings, we conclude that, although ASCs from T2D mice are marginally inferior to control ASCs, they possess comparable therapeutic effects in wound healing.
Deciphering the transcriptional regulatory networks that control size, color, and oil content in Brassica rapa seeds
Authors: Niu Yue , Wu Limin , Li Yanhua , Huang Hualei , Qian Mingchao , Sun Wei , Zhu Hong , Xu Yuanfang , Fan Yonghai , Mahmood Umer , Xu Benbo , Zhang Kai , Qu Cunmin , Li Jiana , Lu Kun 
Published: May 2020

Abstract
Background: Brassica rapa is an important oilseed and vegetable crop species and is the A subgenome donor of two important oilseed Brassica crops, Brassica napus and Brassica juncea. Although seed size (SZ), seed color (SC), and oil content (OC) substantially affect seed yield and quality, the mechanisms regulating these traits in Brassica crops remain unclear.Results: We collected seeds from a pair of B. rapa accessions with significantly different SZ, SC, and OC at seven seed developmental stages (every 7 days from 7 to 49 days after pollination), and identified 28,954 differentially expressed genes (DEGs) from seven pairwise comparisons between accessions at each developmental stage. K-means clustering identified a group of cell cycle-related genes closely connected to variation in SZ of B. rapa. A weighted correlation analysis using the WGCNA package in R revealed two important co-expression modules comprising genes whose expression was positively correlated with SZ increase and negatively correlated with seed yellowness, respectively. Upregulated expression of cell cycle-related genes in one module was important for the G2/M cell cycle transition, and the transcription factor Bra.A05TSO1 seemed to positively stimulate the expression of two CYCB1;2 genes to promote seed development. In the second module, a conserved complex regulated by the transcription factor TT8 appear to determine SC through downregulation of TT8 and its target genes TT3, TT18, and ANR. In the third module, WRI1 and FUS3 were conserved to increase the seed OC, and Bra.A03GRF5 was revealed as a key transcription factor on lipid biosynthesis. Further, upregulation of genes involved in triacylglycerol biosynthesis and storage in the seed oil body may increase OC. We further validated the accuracy of the transcriptome data by quantitative real-time PCR of 15 DEGs. Finally, we used our results to construct detailed models to clarify the regulatory mechanisms underlying variations in SZ, SC, and OC in B. rapa.Conclusions: This study provides insight into the regulatory mechanisms underlying the variations of SZ, SC, and OC in plants based on transcriptome comparison. The findings hold great promise for improving seed yield, quality and OC through genetic engineering of critical genes in future molecular breeding.
Evaluation of the association between presenteeism and perceived availability of social support among hospital doctors in Zhejiang, China
Authors: Xi Xiaoyu , Lu Qianni , Lu Mengqing , Xu Ailin , Hu Hao , Ung Carolina Oi Lam 
Published: May 2020

Abstract
Background: This study investigated the association between presenteeism and the perceived availability of social support among hospital doctors in China. Methods: A questionnaire was administered by doctors randomly selected from 13 hospital in Hangzhou China using stratified sampling. Logit model was used for data analysis. Results: The overall response rate was 88.16%. Among hospital doctors, for each unit increase of the perceived availability of social support, the prevalence of presenteeism was decreased by 8.3% (OR=0.91, P=0.000). In particular, if the doctors perceived availability of appraisal support, belonging support and tangible support as sufficient, the act of presenteeism was reduced by 20.2% (OR=0.806, P=0.000) 20.4% (OR=0.803, P=0.000) and 21.0% (OR=0.799, P=0.000) respectively with statistical differences. Conclusion: In China, appraisal support, belonging support and tangible support, compared to other social support, had a stronger negative correlation with presenteeism among hospital doctors. The benefits of social support in alleviating doctors’ presenteeism warrant further investigation.
Is early integration of palliative home care in oncology treatment feasible and acceptable for advanced cancer patients and their health care providers? A phase 2 mixed-methods study.
Authors: Dhollander Naomi , Smets Tinne , De Vleminck Aline , Lapeire Lore , Pardon Koen , Deliens Luc 
Published: May 2020

Abstract
Background
To support the early integration of palliative home care (PHC) in cancer treatment, we developed the EPHECT intervention and pilot tested it with 32 advanced cancer patients in Belgium using a pre post design. We aim to determine the feasibility, acceptability and perceived effectiveness of the EPHECT intervention.
Methods
Interviews with patients (n = 16 of which 11 dyadic with family caregivers), oncologists and GPs (n = 11) and a focus group with the PHC team. We further analyzed the study materials and logbooks of the PHC team. Preliminary effectiveness was assessed with questionnaires EORTC QLQ C-30, HADS and FAMCARE and were filled in at baseline and 12, 18 and 24 weeks.
Results
Patient reported feelings of safety and control and an optimized quality of life. Being introduced earlier opened space for the PHC team to focus on more than symptom management. Telephone-based contact appeared to be insufficient to support interprofessional collaboration. Furthermore, some family caregivers reported that the nurse of the PHC team was focused little on them.
Conclusion
Nurses of PHC teams are able to deliver early palliative care to advanced cancer patients. However, more attention needs to be given to family caregivers as caregiver and client. Furthermore, the home visits by the PHC team have to be further evaluated and adapted. Lastly, professionals have to find a more efficient way to discuss future care.
The primary total knee arthroplasty: a global analysis
Authors: Gao Jiaxiang , Xing Dan , Dong Shengjie , Lin Jianhao 
Published: May 2020

Abstract
Background: The use of total knee arthroplasty (TKA) in treatment of chronic degenerative pathologies of the knee has boasted of an experience of 50 years. Study objective: The aim of this bibliometric and visualized study is to comprehensively examine the current status and global trends of TKA research.Methods: Publications related to TKA research from 2010 to 2019 were retrieved from Web of Science (WoS) and Science Citation Index-Expanded (SCIE) database, and then analyzed through bibliometric methodology. As for the visualized study, the software VOS viewer was utilized for bibliographic coupling, coauthorship, cociation, and co-occurrence analysis, along with further simulation of publication trends in this field.Results: A total of 8631 publications were eventually included. The number of publications tend to increased annually over worldwide. The United States was the pioneer which has made tremendous contributions, with the most publications and citations, as well as the highest H-index. The JOURNAL OF ARTHROPLASTY has published most papers, while CLINICAL ORTHOPAEDICS AND RELATED RESEARCH has the highest citation frequency. Hospital for Special Surgery have made the greatest contribution when total publication number and coauthorship were taken together. Studies could be divided into five clusters: “alignment study”, “revision TKA study”, “complication study”, “rehabilitation study”, and “perioperative management study”, which have a trend of balanced development in this field.Conclusions: There will be an increasing number of publications on TKA research according to the current global trends, and the United States maintained the leadership in this area. Additionally, a trend of balanced development may exist in the field of TKA research, accompanied with inherent changes of hotspots in each cluster.
The awareness, visibility and support for young carers across Europe: a Delphi study
Authors: Nap Henk Herman , Hoefman Renske , de Jong Nynke , Lovink Lieke , Glimmerveen Ludo , Lewis Feylyn , Santini Sara , D’Amen Barbara , Socci Marco , Boccaletti Licia , Casu Giulia , Manattini Alessandra , Brolin Rosita , Sirk Karina , Hlebec Valentina , Rakar Tatjana , Hudobivnik Tjasa , Leu Agnes , Berger Fabian , Magnusson Lennart , Hanson Elizabeth 
Published: May 2020

Abstract
BackgroundAcross Europe there is limited attention paid in media, policy and empirical research to the situation of young carers (YCs) and their needs for support, while similarly to adult carers, they provide care to ill family members. The Delphi study, which provides the focus for this article, had the overall aim of exploring existing successful strategies to support AYCs (Adolescent Young Carers) aged 15-17 years. Compared to YCs, even less is known about AYCs, a group that is in a critical life transition phase. The study forms part of an EU Horizon 2020 funded research project on AYCs.MethodsA two-round Delphi study was conducted with 66 experts on YCs from 10 European countries. Topics included: (1) visibility and awareness raising of AYCs at local, regional, and national levels, (2) current interventions to support AYCs, and (3) future strategies to support AYCs.ResultsExperts reported that there is a lack of visibility and awareness about YCs in general and AYCs in particular. Although awareness is slowly increasing in most countries, with the UK ranked highest, experts acknowledged that it remains challenging to identify YCs in many countries. Furthermore, the level and type of support available for AYCs differs, with most countries mainly offering support on a local level. Diverse views were expressed regarding future strategies to support AYCs. Experts highlighted the importance of specific legislation to formalize the rights of YCs, and the issue of whether young people should be safeguarded from caregiving or if this is part of regular family life. They also emphasised the relevance of available integrated support services for AYCs, including schools, family, health and social care.Conclusion(s) with key messageIn most European countries, there is a lack of awareness and visibility on young people providing care. Identification of YCs is a crucial first step and a common definition of YCs is called for, together with greater opportunities for young adults to identify themselves as YCs.StatementIdentification alone is not sufficient, rather recognition of YCs by society is needed combined with the necessary resources to ensure integrated AYC friendly support services.
Hypoxia Promotes Differentiation of Pure Cartilage from Human iPS Cells
Authors: Shimomura Seiji , Inoue Hiroaki , Arai Yuji , Nakagawa Shuji , Fujii Yuta , Kishida Tsunao , Shin-Ya Masaharu , Ichimaru Shohei , Tsuchida Shinji , Mazda Osam , Kubo Toshikazu 
Published: May 2020

Abstract
BackgroundWhile cartilage can be formed from induced pluripotent stem cells (iPSCs), challenges such as long culture periods and compromised tissue purity remain. We aimed to determine whether cartilaginous tissue can be produced from iPSCs under hypoxia and to evaluate the effects on the cellular metabolism and purity of the tissue.MethodsHuman iPSCs (hiPSCs) were cultured for cartilage differentiation in monolayers under normoxia or hypoxia (5% O2). We evaluated chondrocyte differentiation by real-time reverse transcription-polymerase chain reaction and fluorescence-activated cell sorting. Then, hiPSCs were cultured for cartilage differentiation in 3D culture under normoxia or hypoxia (5% O2). We evaluated cartilage-like tissues on days 28 and 56 through histological analyses.ResultsHypoxia suppressed the expression of immature mesodermal markers T (Brachyury) and Forkhead box protein F1 (FOXF1) and promoted the expression of the chondrogenic markers aggrecan and CD44. Sex determining region Y- box (SOX) 9-positive cells were increased by culture under hypoxia. Percentages of safranin O-positive and type 2 collagen-positive tissues were increased under hypoxia. Moreover, upon hypoxia-inducible factor (HIF)-1α staining, the nuclear dyeability in tissues cultured under hypoxia was greater than that under normoxia.ConclusionsHypoxia not only led to enhanced cartilage matrix production but also improved cell purity by promoting the expression of HIF-1α. By applying this method, highly pure cartilaginous-like tissues may be produced more rapidly and conveniently.
Motion-preserving treatment of unstable atlas fracture: transoral anterior C1-ring osteosynthesis using a laminoplasty plate
Authors: Zou Xiaobao , Ouyang Beiping , Wang Binbin , Yang Haozhi , Ge Su , Chen Yuyue , Ni Ling , Zhang Shuang , Xia Hong , Wu Zenghui , Ma Xiangyang 
Published: May 2020

Abstract
Background
C1-ring osteosynthesis is a valid alternative to posterior C1–C2 or C0–C2 fusion to preserve important C1–C2 motion in the treatment of unstable atlas fractures. Nevertheless, the fixation instruments used in current studies for transoral anterior C1-ring osteosynthesis were not suitable for anterior anatomy of the atlas or did not have reduction mechanism. We therefore present this report to investigate preliminary clinical effects of transoral anterior C1-ring osteosynthesis using a laminoplasty plate in unstable atlas fractures.
Methods
From January 2014 to December 2017, 13 patients with unstable atlas fractures were retrospectively reviewed. All patients were treated with transoral anterior C1-ring osteosynthesis using a laminoplasty plate. Pre- and postoperative images were obtained to assess reduction of the fracture, internal fixation placement, and bone union. Neurological function, range of motion, and pain levels were evaluated clinically on follow-up.
Results
The surgeries were successfully performed in all cases. The average follow-up duration was 16.6 ± 4.4 months (range 12–24 months). One patient suffered screw loosening after operation and underwent replacement operation subsequently. Satisfactory clinical outcomes were achieved in all patients with ideal fracture reduction, reliable plate placement, well-preserved range of motion, and neck pain alleviation. All patients achieved bone union of fractures without loss of reduction or implant failure or C1–C2 instability during the follow-up. No vascular or neurological complication was noted during the operation and follow-up.
Conclusions
Transoral anterior C1-ring osteosynthesis using a laminoplasty plate is a effective surgical treatment for unstable atlas fractures. This technique has a ingenious reduction mechanism, and can provide satisfactory bone union and preservation of C1–C2 motion.