bluuebunny/crossref_metadata_2025_split huggingface dataset:
Programmed cell senescence in the mouse developing spinal cord and notochord
Authors: Domínguez-Bautista Jorge Antolio , Acevo-Rodríguez Pilar Sarah , Castro-Obregón Susana 
Published: July 2020

AbstractProgrammed cell senescence is a cellular process that seems to contribute to morphogenesis during embryo development, in addition to cell proliferation, migration, differentiation and programmed cell death, and has been observed in evolutionary distant organisms like mammals, amphibians and fish. Programmed cell senescence is a phenotype similar to stress-induced cellular senescence, characterized by the expression of cell cycle inhibitors such as CDKN1A/p21, increased activity of a lysosomal enzyme with beta-galactosidase activity (coined senescence-associated beta-galactosidase) and, most importantly, secretion of growth factors, interleukins, chemokines, metalloproteases, etc., collectively known as a senescent-associated secretory phenotype that instructs surrounding tissue. How wide is the distribution of programmed cell senescence during mouse development and its specific mechanisms to shape the embryo are still poorly understood. Here, we investigated whether markers of programmed cell senescence are found in the developing mouse spinal cord and notochord. We found discrete areas and developmental windows with high senescence-associated beta galactosidase in both spinal cord and notochord; expression of CDKN1A/p21 was documented in epithelial cells of the spinal cord and the notochord. Treatment of mice embryos developed ex-utero in the presence of the senolytic ABT-263 resulted in decrease senescence-associated beta-galactosidase activity and number of motoneurons. Our data suggest that several cell types undergo programmed cell senescence in developing spinal cord and notochord contributing to morphogenesis.Contribution to the Field StatementCellular senescence is a state in which cells no longer divide but have the remarkable ability to secrete signaling molecules that alter the tissue where they reside. In adults, this state is typically induced by stress situations that cause DNA damage so cells with altered genome do not multiply. Senescent cells also form when a tissue is injured; they help to regenerate damaged tissue and contribute to wound healing. Phagocytic cells eliminate them when their function is done, having a transient existence. During vertebrate development some cells acquire a very similar phenotype, coined programmed cell senescence, and interestingly they have been found in regions that organize the pattern of development of some organs. How wide is the distribution of programmed cell senescence during development and how they help to shape the embryo are still poorly understood. We discovered in mice embryos different types of cells with senescent features located in particular regions of the developing nervous system: where motoneurons form and in a region that secrete molecules that instruct the embryo where different types of neurons will be created. We propose that programed cell senescence contributes to the morphogenesis of the nervous system.
CoVaccine HT™ adjuvant potentiates robust immune responses to recombinant SARS-CoV-2 Spike S1 immunisation
Authors: Haun Brien K. , Lai Chih-Yun , Williams Caitlin A. , Wong Teri Ann , Lieberman Michael M. , Pessaint Laurent , Andersen-Elyard Hanne , Lehrer Axel T. 
Published: July 2020

ABSTRACTThe current COVID-19 pandemic has claimed hundreds of thousands of lives and its causative agent, SARS-CoV-2, has infected millions, globally. The highly contagious nature of this respiratory virus has spurred massive global efforts to develop vaccines at record speeds. In addition to enhanced immunogen delivery, adjuvants may greatly impact protective efficacy of a SARS-CoV-2 vaccine. To investigate adjuvant suitability, we formulated protein subunit vaccines consisting of the recombinant S1 domain of SARS-CoV-2 Spike protein alone or in combination with either CoVaccine HT™ or Alhydrogel. CoVaccine HT™ induced high titres of antigen-binding IgG after a single dose, facilitated affinity maturation and class switching to a greater extent than Alhydrogel and elicited potent cell-mediated immunity as well as virus neutralising antibody titres. Data presented here suggests that adjuvantation with CoVaccine HT™ can rapidly induce a comprehensive and protective immune response to SARS-CoV-2.
Rpb4 and Puf3 imprint and post-transcriptionally control the stability of a common set of mRNAs in yeast
Authors: Garrido-Godino A.I. , Gupta I. , Gutiérrez-Santiago F. , Martínez-Padilla A.B. , Alekseenko A. , Steinmetz L.M. , Pérez-Ortín J.E. , Pelechano V. , Navarro F. 
Published: July 2020

ABSTRACTGene expression involving RNA polymerase II is regulated by the concerted interplay between mRNA synthesis and degradation, crosstalk in which mRNA decay machinery and transcription machinery respectively impact transcription and mRNA stability. Rpb4, and likely dimer Rpb4/7, seem the central components of the RNA pol II governing these processes. In this work we unravel the molecular mechanisms participated by Rpb4 that mediate the posttranscriptional events regulating mRNA imprinting and stability. By RIP-Seq, we analyzed genome-wide the association of Rpb4 with mRNAs and demonstrated that it targeted a large population of more than 1400 transcripts. A group of these mRNAs was also the target of the RNA binding protein, Puf3. We demonstrated that Rpb4 and Puf3 physically, genetically, and functionally interact and also affect mRNA stability, and likely the imprinting, of a common group of mRNAs. Furthermore, the Rpb4 and Puf3 association with mRNAs depends on one another. We also demonstrated, for the first time, that Puf3 associates with chromatin in an Rpb4-dependent manner. Our data also suggest that Rpb4 could be a key element of the RNA pol II that coordinates mRNA synthesis, imprinting and stability in cooperation with RBPs.
North American deer mice are susceptible to SARS-CoV-2
Authors: Griffin Bryan D. , Chan Mable , Tailor Nikesh , Mendoza Emelissa J. , Leung Anders , Warner Bryce M. , Duggan Ana T. , Moffat Estella , He Shihua , Garnett Lauren , Tran Kaylie N. , Banadyga Logan , Albietz Alixandra , Tierney Kevin , Audet Jonathan , Bello Alexander , Vendramelli Robert , Boese Amrit S. , Fernando Lisa , Lindsay L. Robbin , Jardine Claire M. , Wood Heidi , Poliquin Guillaume , Strong James E. , Drebot Michael , Safronetz David , Embury-Hyatt Carissa , Kobasa Darwyn 
Published: July 2020

AbstractThe zoonotic spillover of the pandemic SARS-coronavirus 2 (SARS-CoV-2) from an animal reservoir, currently presumed to be the Chinese horseshoe bat, into a naïve human population has rapidly resulted in a significant global public health emergency. Worldwide circulation of SARS-CoV-2 in humans raises the theoretical risk of reverse zoonosis events with wildlife, reintroductions of SARS-CoV-2 into permissive non-domesticated animals, potentially seeding new host reservoir species and geographic regions in which bat SARS-like coronaviruses have not historically been endemic. Here we report that North American deer mice (Peromyscus maniculatus) and some closely related members of theCricetidaefamily of rodents possess key amino acid residues within the angiotensin-converting enzyme 2 (ACE2) receptor known to confer SARS-CoV-2 spike protein binding.Peromyscusrodent species are widely distributed across North America and are the primary host reservoirs of several emerging pathogens that repeatedly spill over into humans includingBorrelia burgdorferi, the causative agent of Lyme disease, deer tick virus, and Sin Nombre orthohantavirus, the causative agent of hantavirus pulmonary syndrome (HPS). We demonstrate that adult deer mice are susceptible to SARS-CoV-2 infection following intranasal exposure to a human isolate, resulting in viral replication in the upper and lower respiratory tract with little or no signs of disease. Further, shed infectious virus is detectable in nasal washes, oropharyngeal and rectal swabs, and viral RNA is detectable in feces and occasionally urine. We further show that deer mice are capable of transmitting SARS-CoV-2 to naïve deer mice through direct contact. The extent to which these observations may translate to wild deer mouse populations remains unclear, and the risk of reverse zoonosis and/or the potential for the establishment ofPeromyscusrodents as a North American reservoir for SARS-CoV-2 is unknown. Nevertheless, efforts to monitor wild, peri-domesticPeromyscusrodent populations are likely warranted as the SARS-CoV-2 pandemic progresses.
TSG Targeting KDM5A affects osteogenic differentiation of bone mesenchymal stem cells induced by bone morphogenetic protein 2
Authors: Wei Min , Jiang Yi , Huang Yuanqing 
Published: July 2020

AbstractTo investigate the effect of 2, 3, 5, 4’-tetrahydroxystilbene-2-O-β-D-glucoside (TSG) on osteogenic differentiation of bone marrow mesenchymal stem cells (BMSC) and its molecular mechanism. The effects of TSG on alkaline phosphatase positive cloning and mineralized nodule formation were also detected. Total mRNA and protein were extracted and effects of TSG on the expression levels of osteopontin (OPN), osteocalcin (OCN), Runt-related transcription factor 2 (Runx2), Osterix and Col1a1 were detected by real-time fluorescence quantitative PCR. Western Blotting was used to detect the inhibitory effect of TSG on KDM5A. BMSCs were transfected with Small interfering RNA (siRNA) targeting KDM5A (si-KMD5A) and pcDNA3.1 KMD5A. TSG significantly increased the activity of ALP and the number of alkaline phosphatase clones and calcified nodule formation. The OPN, OCN, Runx2 and Osterix expression levels were significantly increased among the osteoblasts after TSG treatment. The mechanism study showed that the effect of TSG is realized by inhibiting KDM5A. KDM5A signaling may be involved in the regulation of osteogenic differentiation of rBMSC. TSG can promote osteogenic differentiation and maturation of rBMSC at 0.1-50 μmol / L. The mechanism of action was realized by inhibiting the expression of KDM5A.
Survey on Sharīʿah non-compliant events in Islamic banks in the practice of tawarruq financing in Malaysia
Authors: Ali Mohammad Mahbubi , Hassan Rusni 
Published: July 2020

Purpose
Tawarruq (Islamic commodity financing) has evolved as the most ubiquitous concept in Malaysia’s Islamic banking industry. Nevertheless, the extensive use of tawarruq has invoked a number of Sharīʿah (Islamic law) concerns in its practice. This study aims to investigate the Sharīʿah non-compliant (SNC) phenomena in the practice of tawarruq financing in Malaysia.

Design/methodology/approach
This study adopts qualitative research methodology, combining both descriptive and content analysis. A self-administered questionnaire was distributed to 16 Malaysian Islamic commercial banks to unveil the Sharīʿah non-compliance issues in the application of tawarruq in Islamic banks (IBs) in Malaysia.

Findings
The study found that some practices of tawarruq in Malaysia might not comply with the Sharīʿah, mainly due to the improper sequencing of contracts. The study also discovered that IBs adopt different approaches in dealing with SNC events and the income derived therefrom. Finally, the study noted the influence of board of director/management on certain Sharīʿah decisions particularly on the treatment of non-ḥalāl (impermissible) income.

Practical implications
The findings of the study serve as a reference to industry players and regulators in formulating a Sharīʿah non-compliance risk management framework for tawarruq practices.

Originality/value
The survey on SNC issues in tawarruq practice constitutes the first of its kind in the existing literature.
Promoting family inclusive practice in home treatment teams
Authors: Griffiths Maria , Allen Jo , Patel Krisna , Bell Victoria 
Published: July 2020

Purpose
Families play an instrumental role in helping relatives experiencing mental health issues to stay well. In the context of wider initiatives promoting family and carer needs, this study aims to evaluate the feasibility, acceptability and potential benefits of bespoke training to develop clinicians’ skills in working with families in crisis.

Design/methodology/approach
The study was an uncontrolled evaluation of a one-day workshop for home treatment team staff using pre- and post-questionnaires.

Findings
In total, 83 staff members participated. Overall, there was a strong agreement for the involvement of families, which increased marginally after training. There were significant changes in views about talking to family members without service user consent (p = 0.001) and keeping them informed of their relative’s well-being (p = 0.02). Qualitative feedback indicated that participants enjoyed the interactive elements, particularly role-playing. Training provided an opportunity to practice skills, share knowledge and facilitate the integration of family work into their professional role.

Research limitations/implications
Confident support for families contributes to effective mediation of crisis and continuation of care; factors important in reducing admission rates and protecting interpersonal relationships. Overall, the consistency of responses obtained from participants suggests that this workshop offers a helpful introduction to a family approach at times of a mental health crisis.

Originality/value
This pilot evaluation suggests this new one-day workshop, is a feasible and acceptable training program, which is beneficial in developing clinicians’ skills in working with families in a crisis.
Detection of fraud in high‐quality rice by near‐infrared spectroscopy
Authors: Liu Yachao , Li Yongyu , Peng Yankun , Yang Yanming , Wang Qi 
Published: July 2020

AbstractA key feature of food fraud is the use of a lower value ingredient to imitate an authentic product. This study was based on near‐infrared spectroscopy (NIRS) analysis technology, partial least squares discriminant analysis (PLS‐DA), and a support vector machine (SVM) to detect whether high‐quality rice was mixed with other varieties of rice. As an aid to qualitative discrimination, PLS was used to establish the quantitative analysis model to assist in the recognition of the degree of fraud. Due to the direct correlation between the results of NIRS analysis and the homogeneity of the samples, four groups of samples with different physical forms (full granules, 40 mesh, 70 mesh, and 100 mesh) were prepared, each group consisted of 20 pure samples and 140 mixed samples, and the mixing ratio was between 5% and 50%, with an interval of 5%. Regarding qualitative analysis, the performance of the model has no obvious relationship with the physical state of the sample, the qualitative model of PLS‐DA and SVM can detect the fraudulent rice with a 5% detection limit, respectively. Regarding quantitative analysis, the performance of the prediction model was closely related to the particle size of the samples: 100 mesh > 70 mesh > 40 mesh > full grains. The determination coefficient and root mean square errors of the optimal prediction result were 0.96 and 2.93, respectively. These results demonstrate that NIRS analysis technology is a reliable and fast tool to determine whether high‐quality rice contains other varieties of rice.Practical ApplicationThe work of this article is based on the current background of increasingly serious rice fraud, using near‐infrared spectroscopy to quickly identify fraudulent rice, to a certain extent, and effectively alleviate the rice fraud. This technology can serve for the supervision of food regulatory agencies on rice fraud, and can also be used in food factories to ensure the authenticity of raw materials of rice.
Osteomorphological features of the hind limb bones of Saiga antelope (Saiga tatarica)
Authors: Jing Libaihe , He Peng , Ding Dongfang , Qu Can , Shao Baoping , Ma Jizhong , Wang Jianlin 
Published: July 2020

AbstractThe intralimb indices and calcaneal linear metrics are known as the reliable predictors of locomotor adaptation in artiodactyls. The osteological features of hindlimb in adult Saiga (Saiga tatarica) were described, and its correlation with cursoriality and habitat adaptation was discussed. Gross anatomy data showed Saiga owned the deep acetabulum as a broad lunate surface, the large acetabular anteversion, the well‐developed ischiatic tuberosity and the prominent gluteal lines. It also presented the robust rough line and the strong gluteal

tuberosity. A proximodistally elongated eminence located on the cranially distal tibia, which had not been found in goat. The tibial extensor groove was deep. The calcaneal tuberosity was robust. Digital anatomy data showed Saiga owned the higher metatarsal‐femur ratio than forest musk deer and sheep. Comparing with wild bovids and sheep, Saiga presented a transitional variation in calcaneal form. The mean greatest length of the calcaneus (GLC) and the height of the sustentacular facet (HSF) in Saiga were shorter than that in sheep and longer than that in wild bovids respectively (F = 587.492; F = 10.264, p < .05). The wild bovids had longer cubonavicular facets than the other two groups (F = 18.587, p < .05). The great metatarsal–femur ratio of Saiga implied a superior cursorial ability and high conservation confronting the different habitats. The calcaneal linear metrics might shed light on lifestyle‐related functional adaptation over decades of short‐term evolution in the semi‐free range environment.
Impairment of endoxifen formation in tamoxifen‐treated premenopausal breast cancer patients carrying reduced‐function CYP2D6 alleles
Authors: Thorén Linda , Lindh Jonatan D. , Ackehed Gerd , Kringen Marianne Kristiansen , Hall Per , Bergh Jonas , Molden Espen , Margolin Sara , Eliasson Erik 
Published: July 2020

AimsTamoxifen is bioactivated to endoxifen by polymorphic CYP2D6‐dependent metabolism. Here, endoxifen levels were compared to CYP2D6 diplotypes, tentative target concentrations and side effects.MethodsIn total, 118 Swedish premenopausal breast cancer patients diagnosed 2006–2014, with on‐going postoperative tamoxifen treatment January 2017, were included. Biobanked DNA from peripheral blood was used for CYP2D6 genotyping by TaqMan real‐time polymerase chain reaction (CYP2D6*1, *3, *4, *5, *6, *9, *10, *41, *1xN). Plasma levels of tamoxifen and 3 major metabolites were quantified by liquid chromatography–tandem mass spectrometry. Clinical information on treatment and side effects was retrospectively obtained from medical records.ResultsIn the final analysis of 114 patients, a clear relationship between CYP2D6 genotype and plasma endoxifen levels was evident. Low endoxifen (1.6–5.2 ng/mL), i.e. below the suggested threshold for clinical efficacy, was found in all patients with 2 reduced‐function alleles, 2 null‐alleles, or a null/reduced‐function combination. CYP2D6*41 was the most common reduced‐function allele (82%) and 17 of 21 CYP2D6*41‐carriers exhibited a lower CYP2D6 activity than predicted from published guidelines. No difference in endoxifen levels was observed between carriers of 2 null‐alleles vs patients homozygous for CYP2D6*41 or the corresponding heterozygous combination (P = .338). In patients with endoxifen levels <5.9 ng/mL (36/114), side effects were either mild or absent. At higher endoxifen levels moderate‐to‐severe side effects were reported in a concentration‐dependent manner.ConclusionSignificantly reduced endoxifen levels were observed not only in all homozygous carriers of CYP2D6 null‐alleles, but also in carriers of 2 reduced‐function alleles. This finding may be highly relevant for future, genotype‐based dose considerations.
Monoclonal gammopathy and serum immunoglobulin levels as prognostic factors in chronic lymphocytic leukaemia
Authors: Corbingi Andrea , Innocenti Idanna , Tomasso Annamaria , Pasquale Raffaella , Visentin Andrea , Varettoni Marzia , Flospergher Elena , Autore Francesco , Morelli Francesca , Trentin Livio , Reda Gianluigi , Efremov Dimitar G. , Laurenti Luca 
Published: July 2020

SummaryThe relationship between chronic lymphocytic leukaemia (CLL) and qualitative/quantitative gammaglobulin abnormalities is well established. Nevertheless, in order to better understand this kind of connection, we examined 1505 patients with CLL and divided them into four subgroups on the basis of immunoglobulin (Ig) aberrations at diagnosis. A total of 73 (4·8%), 149 (10%), 200 (13·2%) and 1083 (72%) patients were identified with IgM monoclonal gammopathy (IgM/CLL), IgG monoclonal gammopathy (IgG/CLL), hypogammaglobulinaemia (hypo‐γ) and normal Ig levels (γ‐normal) respectively. IgM paraprotein was significantly associated with a more advanced Binet/Rai stage and del(17p)/TP53 mutation, while IgG abnormalities correlated with a higher occurrence of trisomy 12. Patients with any type of Ig abnormality had shorter treatment‐free survival (TFS) but no significant impact affecting overall survival (OS) compared to those with normal Ig levels.
Sequence stratigraphy of a Middle to Upper Ordovician foreland succession (Ottawa Embayment, central Canada): Evidence for tectonic control on sequence architecture along southern Laurentia
Authors: Oruche Nkechi E. , Dix George R. 
Published: July 2020

AbstractThe Middle to Upper Ordovician foreland succession of the Ottawa Embayment in central Canada is divided into nine transgressive‐regressive sequences that defines net deepening of a platform succession over ~15 m.y. from peritidal to outer ramp settings, then a return to peritidal conditions over ~3 m.y. related to basin filling by orogen‐derived siliciclastics. With a backdrop of net eustatic rise through the Middle to Late Ordovician, there are several different expressions of structural influence on sequence development in the embayment. During the Middle Ordovician (Darriwilian), foreland‐basin initiation was marked by regional onlap with abundant synsedimentary deformation across a faulted trailing‐margin platform interior; subsequent craton‐interior uplift resulted in voluminous influx of siliciclastics contemporary with local structurally influenced local channelization; then, a formation of a platform‐interior shale basin defines continued intrabasin tectonism. During the Late Ordovician (Sandbian, early Katian), structural influence was superimposed on sea‐level rise as indicated by renewed local development of a platform‐interior shale basin; differential subsidence and thickness variation of platform carbonate successions; abrupt deepening across shallow‐water shoal facies; and, micrograben development coincident with foreland‐platform drowning. These stratigraphic patterns are far‐field expressions of distal orogen development amplified in the platform interior through basement reactivation along an inherited buried Precambrian fault system. Comparison of Upper Ordovician (Sandbian‐lower Katian) sequence stratigraphy in the Ottawa Embayment with eustatic frameworks defined for the Appalachian Basin reveals greater regional variation associated with Sandbian sequences compared to regional commonality in base level through the early Katian.
Gender Discrimination, Perceived School Unfairness, Depressive Symptoms, and Sleep Duration Among Middle School Girls
Authors: N. Bell Ariana , Juvonen Jaana 
Published: July 2020

The current study examines how gender discrimination by adults in school is linked with depressive symptoms and sleep duration over time in middle school. The main goal is to test one psychological mechanism that can account for such associations: perceived school unfairness. Relying on a racially‐ethnically diverse sample of girls (N = 2,718, Mage = 13.01, SDage = 0.39) from 26 middle schools, multilevel mediation analyses revealed that girls who experienced school‐based gender discrimination by an adult in seventh grade reported higher levels of perceived school unfairness in eighth grade. Moreover, perceived unfairness, in turn, was associated with more depressive symptoms and shorter sleep durations by eighth grade. Implications of changes in adolescent girls’ sleep and mood related to their experiences of gender discrimination are discussed.