bluuebunny/crossref_metadata_2025_split huggingface dataset:
MicroRNA-4651 represses hepatocellular carcinoma cell growth and facilitates apoptosis via targeting FOXP4
Authors: Li Yun , Wang Xiaoying , Li Zhonggang , Liu Bingxia , Wu Chaoyu 
Published: May 2020

Abstract
MicroRNAs (miRNAs) belong to the subgroup of small noncoding RNAs, which typically serve as important gene regulators to participate in different biological events, such as tumor cell growth and apoptosis. Recent studies indicated microRNA-4651 (miR-4651) was involved in hepatocellular carcinoma (HCC) progression. The certain role of miRNA-4651 during the progression of HCC, however, remains unclear. Herein, we investigated the mRNA expression level of miR-4651 in HCC tissues and HCC cell lines and found miR-4651 was noticeably down-regulated compared with the normal liver tissues and QSG-7701 cell line, respectively. Then, miR-4561 overexpression obviously repressed the proliferation and promoted apoptosis in two HCC cell lines. Interestingly, we further identified that miR-4561 could directly interact with FOXP4 in HCC cells by using bio-informatic method and report assay. Moreover, forced expression of FOXP4 showed an opposite effect compared with miR-4561 in HCC cell lines. Hence, our findings strongly indicated that miR-4561 regulated the HCC cell growth and apoptosis mainly through targeting the FOXP4 genes. Clinically, the miR-4561/FOXP4 axis might be a potential target for therapeutic application of HCC patient treatment.
Involvement of amylin B-H2S-connexin 43 signaling pathway in vascular dysfunction and enhanced ischemia–reperfusion-induced myocardial injury in diabetic rats
Authors: Liu Xiaoyong , Yang Rui , Bai Wenwei , Xu Xiang , Bi Feng , Hao Yingzheng , Yang Qishi , Li Hu 
Published: May 2020

Abstract
The present study was designed to investigate the role of amylin, H2S, and connexin 43 in vascular dysfunction and enhanced ischemia–reperfusion (I/R)-induced myocardial injury in diabetic rats. A single dose of streptozotocin (65 mg/kg) was employed to induce diabetes mellitus. After 8 weeks, there was a significant decrease in the plasma levels of amylin, an increase in I/R injury to isolated hearts (increase in CK-MB and cardiac troponin release) on the Langendorff apparatus. Moreover, there was a significant impairment in vascular endothelium function as assessed by quantifying acetylcholine-induced relaxation in norepinephrine-precontracted mesenteric arteries. There was also a marked decrease in the expression of H2S and connexin 43 in the hearts following I/R injury in diabetic rats. Treatment with amylin agonist, pramlintide (100 and 200 µg/kg), and H2S donor, NaHS (10 and 20 μmol/kg) for 2 weeks improved the vascular endothelium function, abolished enhanced myocardial injury and restored the levels of H2S along with connexin 43 in diabetic animals. However, pramlintide and NaHS failed to produce these effects the presence of gap junction blocker, carbenoxolone (20 and 40 mg/kg). Carbenoxolone also abolished the myocardial levels of connexin 43 without affecting the plasma levels of amylin and myocardial levels of H2S. The decrease in the amylin levels with a consequent reduction in H2S and connexin 43 may contribute to inducing vascular dysfunction and enhancing I/R-induced myocardial injury in diabetic rats.
Exploring the mechanism of Shengmai Yin for coronary heart disease based on systematic pharmacology and chemoinformatics
Authors: Jiang Yan , He Qi , Zhang Tianqing , Xiang Wang , Long Zhiyong , Wu Shiwei 
Published: May 2020

Abstract
Objective: To explore the mechanism of Shengmai Yin (SMY) for coronary heart disease (CHD) by systemic pharmacology and chemoinformatics.
Methods: Traditional Chinese Medicine Systems Pharmacology Database (TCMSP), traditional Chinese medicine integrative database (TCMID) and the traditional Chinese medicine (TCM) Database@Taiwan were used to screen and predict the bioactive components of SMY. Pharmmapper were utilized to predict the potential targets of SMY, the TCMSP was utilized to obtain the known targets of SMY. The Genecards and OMIM database were utilized to collect CHD genes. Cytoscape was then used for network construction and analysis, and DAVID was used for Gene Ontology (GO) and pathway enrichment analysis. After that, animal experiments were then performed to further validate the results of systemic pharmacology and chemoinformatics.
Results: Three major networks were constructed: (1) CHD genes’ protein–protein interaction (PPI) network; (2) SMY–CHD PPI network; (3) SMY known target–CHD PPI network. The other networks are minor networks generated by analyzing the three major networks. Experimental results showed that compared with the model group, the Shengmai injection (SMI) can reduce the myocardial injury score and the activities of serum aspartate aminoconvertase (AST), CK and lactate dehydrogenase (LDH) in rats (P&lt;0.05), and reduce serum lipid peroxide (LPO) content and increase serum superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities in myocardial infarction rats (P&lt;0.05). SMI can also decrease the expression of MMP-9 mRNA and increase that of TIMP-1 mRNA (P&lt;0.01).
Conclusion: SMY may regulate the signaling pathways (such as PPAR, FoxO, VEGF signaling), biological processes (such as angiogenesis, blood pressure formation, inflammatory response) and targets (such as AKT1, EGFR, MAPK1) so as to play a therapeutic role in CHD.
Real-time diagnosis of sentinel lymph nodes involved to breast cancer based on pH sensing through lipid synthesis of those cells
Authors: Miripour Zohreh S. , Aghaee Parisa , Abbasvandi Fereshteh , Hoseinpour Parisa , Parniani Mohammad , Abdolahad Mohammad 
Published: May 2020

Abstract
Lipid synthesis is the recently found metabolism of cancer cells after their metastasis to lymph nodes (LNs). Carbonic acid is the main byproduct of the lipid metabolism in such cells which resulted in acidification of LN ambient. Hence, calibrated pH sensing could be a diagnostic method to find involved LNs. Here, we designed a simple pH sensing method by a syringe containing sterile PBS and embedded by litmus paper to intraoperatively check the pH of LN fluid. Injected phosphate buffer saline (PBS) would homogenize the LN fluid and litmus needle would detect the pH of the LN. We presented an experimental pathological calibration for the pH values in correlation with cancerous states of the LNs. This system named metabolism based metastatic lymph diagnoser (MMLD) could be a real-time noninvasive tool for precise and fast diagnosis of involved LNs.
Etiology of Childhood Bilateral Sensorineural Hearing Loss in Shandong Province, China
Authors: Jiang Fan , Kuper Hannah , Bright Tess , Qin Wen-Zhe 
Published: May 2020

Objectives
The purpose of this study is to ascertain the etiology of bilateral sensorineural hearing loss (SNHL) in children aged ≤ 18 years living in Shandong province.

Method
Data were taken from a cross-sectional study, which was conducted between 2015 and 2017. The study included children aged ≤ 18 years, recruited from special schools for children with hearing loss and from hearing rehabilitation centers in Shandong province of China. Children were screened for bilateral SNHL through audiological testing. Clinical examination, genetic testing, and structured interviews were conducted for those children who were identified as having hearing loss to identify the potential cause.

Results
The etiology of bilateral SNHL in our sample was genetic in 874 (39.3%), acquired in 650 (29.3%), and unknown in 697 (31.4%) children. Among children with acquired SNHL, the cause was maternal viral infection in 75 (11.5%); perinatal factors in 238 (36.6%); meningitis, measles, and mumps in 146 (22.5%); and ototoxic exposure in 117 (18%) children. Among the children with genetic SNHL, only 44 (4.9%) were identified as having syndromic hearing loss, and the remainder (95.1%) were classified as nonsyndromic hearing loss.

Conclusion
The findings indicated that nearly 30% of bilateral SNHL in Shandong province could be preventable through immunization, early prenatal diagnosis, proper treatment of infections, and avoidance of prescription of ototoxic drugs. This finding emphasizes the need for programs aimed at improving the health services at primary and secondary levels of health care, which will in turn prevent childhood hearing loss.
Executive Control in Adults Who Stutter: The Antisaccade Task
Authors: Gkalitsiou Zoi , Byrd Courtney , Griffin Zenzi 
Published: May 2020

PurposeThe purpose of this study was to investigate executive control in adults who stutter (AWS) and adults who do not stutter (AWNS) via a nonspeech paradigm, wherein eye movements were monitored (i.e., antisaccade task). Processes involved in an antisaccade task include working memory, attention, and voluntary motor control, but the task primarily provides insight into inhibitory control.MethodSeventeen AWS (14 men, three women;M= 23.41 years) and 17 AWNS (M= 23.29 years) were presented with a combination of prosaccade (i.e., looking toward a target) and antisaccade (i.e., suppress a reflexive saccade toward the target and look in the opposite direction) trials. The distance of the target from the center of the screen was also manipulated (i.e., 5.5o= short distance and 10.8o= long distance). Data for accuracy and reaction time of the first accurate saccade were collected and analyzed.ResultsNo difference was found between AWS and AWNS in accuracy or in reaction time. Both groups were more accurate in the prosaccade than the antisaccade trials and in the long compared to the short distance trials. Furthermore, both groups demonstrated longer saccade latencies for long compared to short distances and for antisaccade compared to prosaccade trials.ConclusionsPreliminary results do not support deficits in inhibition in AWS during a motorically simple, non–speech-related oculomotor task, but additional research is warranted.
Navigating the “Passaggio” Between Voice Therapy and a Singing Lesson: Ethical Considerations for the Speech-Language Pathologist
Authors: McConville Katherine 
Published: May 2020

Purpose
The purpose of this article is to discuss factors that guide ethical decision making in determining what constitutes medically necessary voice therapy that involves or targets singing, which is distinct from a singing lesson.

Method
Different treatment tasks and scenarios are identified, and their rationales are compared with deference to precedent literature and relevant portions of the American Speech-Language-Hearing Association Code of Ethics. These materials are referenced in an effort to define the boundaries between voice therapy involving singing technique and a singing lesson.

Results
Suggestions regarding goal writing and documentation to highlight apparent boundaries are made.

Conclusions
In the care of injured voices, both precedent and ethical principles support speech-language pathology treatment that targets singing. However, it is incumbent upon the treating speech-language pathologist to determine when they are venturing into tasks that no longer serve a therapeutic purpose and to refer the patient for voice lessons as appropriate.
Collaboration in Schools: Let's Define It
Authors: Mitchell Mary P. , Ehren Barbara J. , Towson Jacqueline A. 
Published: May 2020

PurposeThe purpose of this article is to guide professionals, including speech-language pathologists (SLPs) working in schools and those in academe, to operationally define the type of collaboration they are targeting. As professionals collaborate to support students who struggle with the demands of academic reading, writing, listening, and speaking, it is imperative to differentiate the type of working together necessary to meet the needs of the students they serve. The lack of a definition in research literature and practice is problematic for a variety of stakeholders, including practitioners, administrators/supervisors, professional learning facilitators, university instructors, researchers, and authors of professional literature. The authors offer as an example their work in operationally defining a specific kind of collaboration called “shared-creation collaboration.” They situate shared-creation collaboration on one end of a working-together continuum. Then, they detail why and how they used the conceptual framework of an Innovation Configuration (IC) Map, developed by Hall and Hord (2015), to create an IC Map for SLPs and teachers to use for shared-creation collaboration. Additionally, they offer scenarios to illustrate a range of interactions in which SLPs might engage in schools.ConclusionsThe SLP/Teacher Shared-Creation Collaboration IC Map and working-together continuum are examples of two tools that can guide school professionals to design and implement working-together practices that match specific purposes and students' needs. However, many configurations of working together are possible and worthwhile; stakeholders can advance the work of assisting learners who struggle by defining their practices specifically.
Novel approach for automatic mid‐diastole frame detection in 2D echocardiography sequences for performing planimetry of the mitral valve orifice
Authors: Faraji Mahtab , Behnam Hamid , Norizadeh Cherloo Mohammad , Shojaeifard Maryam 
Published: May 2020

The mitral valve orifice area is a reliable measure for evaluating mitral valve stenosis (MS) severity, which is obtained by the planimetry of the mid‐diastole frame in the echocardiography sequences. Since the manual method for determining this frame is time‐consuming and user‐dependent, a novel automatic method has been proposed in this study. First, the region of interest (ROI) containing the mitral valve orifice region is detected using circular Hough transform and k‐means algorithms. Then, the dimension reduction method is applied to the ROI of each frame to map it into a point in a 2D space. The performance of the local linear embedding (LLE), isometric mapping, kernel principal component analysis (PCA), and linear PCA algorithms has been evaluated in this study. Finally, a distance curve is obtained by calculating the Euclidean distance between consecutive points in 2D space, and the mid‐diastole frame is determined by interpreting this curve. The proposed algorithm was validated using 2D echocardiography of the 20 MS patients. Finally, the LLE method showed the best result, and the average frame difference for 20 cases using the proposed method compared with the gold standard (the echo‐cardiologist opinion) was 1.40.
Multimodality Approach in Detection and Characterization of Hepatic Metastases
Authors: Patil Pooja G. , Reddy Pramesh , Rawat Sudarshan , Ananthasivan Rupa , Sinha Rakesh 
Published: May 2020

AbstractEarly detection of liver metastases is important in patients with known primary malignancies. This plays an important role in treatment planning and impacts on further management of certain primary malignancies.Magnetic resonance imaging (MRI), computed tomography (CT), and positron emission tomography-computed tomography scans are reported to have high accuracy in the diagnosis of intrahepatic lesions. MRI in particular has the advantages of its high tissue sensitivity and its multiparametric approach.Hepatic metastatic lesions have considerable overlap in their radiological appearance, and in this article the imaging appearance of various hepatic metastasis and approach is described.
Efficacy of Platelet-rich Plasma for Low Back Pain: A Systematic Review and Meta-analysis
Authors: Xuan Zhaopeng , Yu Wenjun , Dou Yichen , Wang Tao 
Published: May 2020

AbstractBackground Platelet-rich plasma (PRP) may be beneficial for patients with low back pain. However, the results remain controversial. We conducted a systematic review and meta-analysis to explore the efficacy of PRP for low back pain.Methods PubMed, Embase, Web of Science, EBSCO, and Cochrane Library databases were searched systematically. Randomized controlled trials (RCTs) assessing the effect of PRP on low back pain were included. Two investigators independently searched articles, extracted data, and assessed the quality of included studies. The primary outcome was pain scores within 8 weeks. Meta-analysis was performed using the random-effects model.Results Three RCTs involving 131 patients were included in the meta-analysis. Overall, compared with control intervention for low back pain, PRP injection was found to reduce pain scores significantly (mean difference: − 1.47; 95% confidence interval [CI], − 2.12 to − 0.81; p < 0.0001), improve the number of patients with > 50% pain relief at 3 months (risk ratio [RR]: 4.14; 95% CI, 2.22–7.74; p < 0.00001), and offer relatively good patient satisfaction (RR: 1.91; 95% CI, 1.04–3.53; p = 0.04). No increase in adverse events was reported after PRP injection (RR: 1.92; 95% CI, 0.94-3.91; p = 0.07).Conclusions Compared with control intervention for low back pain, PRP injection was found to improve pain relief and patient satisfaction significantly with no increase in adverse events.
Negative Pressure Dressings over Free Muscle Flaps with Immediate Split-Thickness Skin Grafting: A 9-Year Experience
Authors: Kumbla Pallavi A. , Henry Steven L. , Boyd Carter J. , Kelley Patrick K. , Thorburn Ashley Q. , Myers René P. 
Published: May 2020

Abstract
Background Negative pressure wound therapy has allowed for significant advances in the treatment of wounds. This occurs through a process of angiogenesis, microdeformation, macrodeformation, and decrease in exudate and bacterial load. It is routinely used as a bolster in the management of skin grafts. However, its role as a dressing over free muscle flaps has not gained widespread acceptance due to the fear of flap compromise.
Methods A retrospective review of 97 patients over 9 years was performed. All patients underwent free muscle flap coverage of various wounds with immediate split-thickness skin grafting. A negative pressure dressing was applied, with windows made in the foam sponge to enable Doppler monitoring as well as visual inspection of the flap. Complications including flap failure, skin graft loss, hematoma, distal flap necrosis, negative pressure dressing failure, partial muscle necrosis, and mild flap congestion were assessed.
Results Flap loss occurred at a rate of 8.2% (eight flaps). Four of these flaps were lost due to patient factors not attributable to the dressing. In the remaining four flaps (4.1% of the series), it is conceivable that the negative pressure dressing was a contributing factor. However, this failure rate is comparable to flap loss rates in studies where negative pressure dressings were not used. The rates of skin graft failure, hematoma, distal flap necrosis, inability to maintain seal, partial muscle necrosis, and mild flap congestion were also acceptable and similar to studies where negative pressure dressings were not used.
Conclusion Negative pressure dressings over free muscle flaps with immediate split-thickness skin grafts are effective and safe to use while allowing for postoperative flap monitoring and skin graft protection.
Knockdown of KLF9 promotes the differentiation of both intramuscular and subcutaneous preadipocytes in goat
Authors: Xu Qing , Lin Yaqiu , Wang Yong , Bai Wenlin , Zhu Jiangjiang 
Published: May 2020

AbstractKLF9 is reported to promote adipocyte differentiation in 3T3-L1 cells and pigs. However, the roles of KLF9 in adipocytes differentiation of goat remain unknown. In this study, the expression profiles of KLF9 were different between subcutaneous and intramuscular preadipocytes of goat during differentiation process. After silencing KLF9 gene, the lipid droplets were increased in both two types of adipocytes. In subcutaneous preadipocyte with silencing KLF9, the expressions of C/EBPβ, PPARγ, LPL, KLF1-2, KLF5, and KLF17 genes were up-regulated, while KLF12, KLF4, and KLF13 genes were down-regulated in expression level. In intramuscular preadipocyte, aP2, C/EBPα, KLF2-3, KLF5, and KLF7 gene were up-regulated, and Pref-1 gene was down-regulated. In addition, the binding sites of KLF9 existed in the promoters of aP2, C/EBPα, C/EBPβ, LPL and Pref-1. Taken together, KLF9 play a negative role in the differentiation of both intramuscular and subcutaneous preadipocytes in goats, but the functional mechanism may be different.
Autoionization dynamics of helium nanodroplets resonantly excited by intense XUV laser pulses
Authors: Ovcharenko Y , LaForge A C , Langbehn B , Plekan O , Cucini R , Finetti P , O’Keeffe P , Iablonskyi D , Nishiyama T , Ueda K , Piseri P , Di Fraia M , Richter R , Coreno M , Callegari C , Prince K C , Stienkemeier F , Möller T , Mudrich M 
Published: May 2020

Abstract
The ionization dynamics of helium droplets irradiated by intense, femtosecond extreme ultraviolet (XUV) pulses is investigated in detail by photoelectron spectroscopy. Helium droplets are resonantly excited to atomic-like 2p states with a photon energy of 21.5 eV and autoionize by interatomic Coulombic decay (ICD). A complex evolution of the electron spectra as a function of droplet size (250 to 106 He atoms per droplet) and XUV intensity (109–1012 W cm−2) is observed, ranging from narrow atomic-like peaks that are due to binary autoionization, to an unstructured feature characteristic of electron emission from a nanoplasma. The experimental results are analyzed and interpreted with the help of a numerical simulation based on rate equations taking into account all relevant processes—multi-step ionization, electronic relaxation, ICD, secondary inelastic collisions, desorption of electronically excited atoms, and collective autoionization (CAI).
Spectroscopy properties of a single praseodymium ion in a crystal
Authors: Xia Kangwei , Kolesov Roman , Wang Ya , Siyushev Petr , Kornher Thomas , Reuter Rolf , Yang Sen , Wrachtrup Jörg 
Published: May 2020

Abstract
Addressing and coherent control of single atoms in solids, with both optical and nuclear spin degrees of freedom is of particularly interest for applications ranging from nanoscale sensing to quantum computing. Here, we performed the spectroscopy study of single praseodymium ions in an yttrium aluminum garnet crystal at cryogenic temperature. The single nuclear spin of individual praseodymium ions is detected through a background-free optical upconversion readout technique. Single ions show stable photoluminescence with spectrally resolved hyperfine splitting of the praseodymium ground state. Based on this measurement, optical Rabi and optically detected nuclear magnetic resonance measurements are performed to study their spin coherence properties. We find short the spin coherence times of praseodymium nuclear spins which we attribute to spin phonon coupling.