bluuebunny/crossref_metadata_2025_split huggingface dataset:
Application of Internet of Things in Cell-Based Therapy Delivery: Protocol for a Systematic Review
Authors: Lam Ching , van Velthoven Michelle Helena , Meinert Edward 
Published: December 2019

Background
Internet of Things (IoT), or Industry 4.0, represents a smart shift to more interconnected manufacturing processes where individual entities within the supply chain communicate with each other to achieve greater flexibility and responsiveness in general manufacturing and leaner manufacturing to reduce the cost of production. IoT has become instrumental in driving leaner manufacturing and more efficient systems in other industries such as transportation and logistics. Cell-based therapeutic products could potentially transform various diseases; however, the delivery of these products is complex and challenging.

Objective
This study aims to understand the applicability of IoT in cell-based product supply chains and delivery.

Methods
We will search Medline, EMBASE (OvidSP), Web of Science, Cochrane Library &amp; HEED, Scopus, ACM digital library, INSPEC, ScienceDirect, and the IEEE Xplore Digital Library for studies published after 2008 using a combination of keywords and subject headings related to IoT used in cell therapies. Additionally, a Google search to identify gray literature will be conducted. Two authors will independently screen the titles and abstracts identified from the search and accept or reject the studies according to the study inclusion criteria. Any discrepancies will then be discussed and resolved. The quality of the selected literature will be assessed using the Critical Appraisal Skills Programme systematic review checklist.

Results
Data from eligible publications will be abstracted into a predesigned form to map the current and future directions of the technologies, applications, benefits, and challenges in the implementation of IoT in regenerative medicine. This study will be published in a peer-reviewed journal in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines. This systematic review will be executed by June 2020, and the completed review will be published in a peer-reviewed journal to inform future developments in IoT application for the delivery of cell-based therapies.

Conclusions
This review paper will provide an overview of all technologies available in the area and inspect the current IoT applications in cell-based therapies to identify the benefits, challenges, and future directions of using IoT to allow safe and cost-effective delivery of cell-based therapies.

International Registered Report Identifier (IRRID)
PRR1-10.2196/16935
WHAT HAPPENS AFTER THEY LAUGH: HOW HUMOROUS ADVERTISEMENTS HAVE AN EFFECT ON CONSUMERS’ ATTITUDES, WORD OF MOUTH INTENTIONS, AND PURCHASE INTENTIONS, WITH THE NEED FOR HUMOR PLAYING A MODERATING ROLE
Authors: Primanto Alfian Budi , Dharmmesta Basu Swastha 
Published: December 2019

Introduction/Main Objectives: Even thought there is a risk from using humor as an advertising campaign strategy in industries which rely on trust and their technological prowess, like the telecommunications sector, the fact that there are a great number of humorous advertisements arranged by telecommunications companies in Indonesia needs to be researched. Background Problems: This research aims to narrow the phenomenon by investigating the effect of humorous advertisements with the need for humor as a moderating role on consumers’ attitudes, consumers’ intent to purchase, and their word of mouth intention. Novelty: By adding the need for humor in a moderating role, and the word of mouth intention as an endogenous variable, this research aims to extend the existing models of humorous advertising’s effectiveness. Research Methods: The research was designed to focus on consumers who can be classified as young adults (18 to 34 years old) and are not users of the mobile SIM card brands that the ad they perceived as funny ones. The researcher conducted a survey with a five point Likert scale, an online questionnaire, and purposive sampling as the primary data collection methods. Finding/Results: The result shows that although a humorous advertisement has a significant impact on the attitude toward the advertisement and the word of mouth intention, the humorous advertisement has no significant impact on the attitude toward the brand and the consumers’ intent to purchase. The need for humor failed to moderate the relationship between the humorous advertisement and the attitude toward the advertisement in this study. Conclusion: The researcher concludes that the impact of a humorous advertisement was limited to only entertaining the consumers, and to encourage their word of mouth intention, not their purchase intention.
THE EFFECT OF LIMAN LEAVES EXTRACT ON FETAL RAT DEVELOPMENT
Authors: METI WIDIYA LESTARI  , IRDA FIDRIANNY  , ANDREANUS A SOEMARDJI  
Published: December 2019

Objective: The objective of the study was to observe the effect of liman leaves extract on fetal rat development and provide safety information for its use during pregnancy.
Methods: Estrus cycles of female Wistar rats were observed and mated to male rats, 0 day of pregnancy determined after finding a vaginal plug or in a vaginal smear, there was sperm. Sample was administered orally using sonde with a single administration on the 11th day of pregnancy, at doses of 3750 mg/kg bw, 1185 mg/kg bw, and 375 mg/kg bw. On the 19th day of pregnancy, the rats were sacrificed and then observed the number of implantation, corpus luteum, intrauterine death, and fetal abnormalities.
Results: The dose of 375 mg/kg bw exposed the highest average implantation rate (13.20%) and the largest number of corpus luteum (13.90%). The highest total intrauterine death was presented by dose of 3750 mg/kg bw and significantly different (p<0.05) compared to the control group. The dose of 375 mg/kg bw expressed the highest percentage of embryos with resorption (19.03%), while the lowest average fetal body was shown by 3750 mg/kg bw compared to the other groups. The highest percentage of external abnormalities was given by the dose of 375 mg/kg bw (12.91%), which abnormalities found were dwarf, cleft palate, hydrocephalus, short sleeve, and hematoma.
Conclusion: Liman leaves extract was mild teratogenic on Wistar rat fetus.
PRELIMINARY PHYTOCHEMISTRY AND ANTIDIABETIC ACTIVITY OF PORTULACA GRANDIFLORA HOOK PLANT EXTRACT ON STREPTOZOTOCIN-INDUCED DIABETES IN RATS
Authors: DEVI M  , KOMAL S  , LOGESHWARI B  
Published: December 2019

Objective: The present study was aimed to evaluate the antidiabetic activity of ethanolic extract of the whole aerial plant of Portulaca grandiflora Hook on streptozotocin (STZ)-induced diabetic rats.
Methods: Experimental diabetes was induced by a single dose of intraperitoneal injection of STZ (150 mg/kg). Adult male Wister albino rats were divided into five groups; normal control, diabetic control, diabetic glibenclamide (5 mg/kg), diabetic P. grandiflora H. extract (200 mg/kg), and diabetic P. grandiflora H. extract (400 mg/kg) for 21 days and analyzed for body weight (BW) and blood glucose.
Results: The STZ-treated diabetic control rats showed a significant increase in blood glucose with a concomitant decrease in BW. Oral administration of P. grandiflora H. extract (200 and 400 mg/kg) and glibenclamide (5 mg/kg) for 21 days showed a significant reduction in blood glucose levels and elevation in the bodyweight studies as compared to control and glibenclamide-treated rats.
Conclusion: The results of the present study showed that a potent antidiabetic activity was present in the aerial part of plant P. grandiflora H. extract.
CHEMICAL CONSTITUENTS, ANTIOXIDATIVE, CYTOTOXIC, AND GENOTOXIC EFFECTS OF MISCANTHUS CAPENSIS ROOTS EXTRACT
Authors: IDOWU JONAS SAGBO  , WILFRED OTANG MBENG  
Published: December 2019

Objective: Miscanthus capensis (Nees) Andersson (Poaceae family) is used in traditional medicine to treat pimples, wounds, eczema, acne, and other ailments. The present study investigates the possible chemical constituents, antioxidant, cytotoxic, and genotoxic effects of the methanol extract of the M. capensis roots.
Methods: Gas chromatography-mass spectrometry (GC-MS) analysis was carried out to identify the chemical constituents of the plant extract. Cytotoxicity and mode of cell death toward human dermal fibroblasts (MRHF) cells were assessed using the ImageXpress® Micro XLS analysis system. Genotoxic effect toward Vero cells was also investigated using micronucleus assay. Furthermore, ferric reducing power (Ferric reducing antioxidant potential [FRAP]) and reactive oxygen species (ROS) levels in MRHF cells were used to estimate the antioxidant activity of the plant extract.
Results: The GC-MS results showed a maximum amount of bioactive components (77 compounds) with rosifoliol (33.66%) being the major component detected. However, the extract was not toxic to MRHF cells but demonstrated anti-proliferation rather than cell death at the tested concentrations (25, 50, and 100 μg/ml). In addition, the plant extract also displayed non-genotoxic toward Vero cells with no significant increase in micronucleus formation at the tested concentration while a concentration dependence response in FRAP values and ROS levels in MRHF cells lines were observed indicating better antioxidant activities of the plant extract.
Conclusion: The results suggest that M. capensis could be a promising candidate for preventing or eradicating skin and oxidative-stress related diseases.
THE ROLE OF YOGA THERAPY IN THE MANAGEMENT OF BRONCHIAL ASTHMA (TAMAKA SHWASA)
Authors: ARPAN A BHATT  , SHWETA PANDEY  , BRAJESH SINGH  , GAURAVI VYAS  , GYANENDRA DATTA SHUKLA  , UPASNA GULATI  
Published: December 2019

Objective: The present study was conducted on patients taken from Swasthavrutta department of Gujarat Ayurved University in Jamnagar town, Gujarat. The study was done with an objective of providing a safe and reliable as well as cost-effective natural management of symptoms occurring in patients of bronchial asthma. This is also done to reduce the various harmful effects of modern medicines among the patients and to reduce the use and dependency on inhalers.
Methods: Thirty-two patients with bronchial asthma (Tamaka Shwasa) underwent training for 16 weeks in an integrated set of yoga exercises, including prayer, strengthening exercises, Yogasanas (including standing Asana, prone Asana, sitting Asana, and supine Asana), Pranayama (Nadi Shodhana, Ujjayi, Bhramari, and Aumkara Dharana), and Shuddhi Kriya (to be done once in a week) which includes Kunjal, Sutraneti, and Kapalabhati. The patients were told to practice these exercises for 60 min daily.
Results: Significant improvement was seen in the patients who undergone yogic interventions. There was significant improvement in the duration of Shwasakashtata, frequency of Shwasakashtata, reduction in taking number of emergency medicine, Pinasa, Kaphashtheevana, cough, Ghurghuraka, orthopnea, Parshvashula, Lalate Sweda, result on peak expiratory flow rate, airway exchange catheters, oxyhemoglobin saturation, and chest expansion.
Conclusion: From the study, it can be concluded that yoga practices can be opted as long-term management of bronchial asthma, but its beneficial effects need to be studied in more details on large sample size.
ANTITUMOR ACTIVITY OF CRATEVA MAGNA IN TRANSPLANTABLE TUMOR MODELS IN MICE
Authors: ANKITA DEEP  , SINDHU R  , KENGANORA MRUTHUNJAYA  , SANTHEPETE N MANJULA  
Published: December 2019

Objective: The present study aims to evaluate the anticancer and immunomodulatory activity of both the alcoholic and aqueous extracts of Crateva magna (ALCM and AQCM) in solid tumor model and ascites tumor model in mice.
Methods: The study was divided into in vitro and in vivo sections. In vitro, antioxidant activity of both the extracts was evaluated by well-established antioxidant assays such as α, α-diphenyl-β-picrylhydrazyl radical scavenging, reducing power, and 3-ethylbenzothiazoline-6-sulfonic acid radical scavenging. Both the extracts were subjected for in vitro preliminary cytotoxicity screening by brine shrimp lethality assay and Trypan blue exclusion assay. In vivo antitumor activity of ALCM and AQCM was assessed at the doses of 100 mg/kg and 200 mg/kg bodyweight, respectively, by Dalton’s Lymphoma ascites induced robust tumor model and Ehrlich Ascites Carcinoma induced ascites tumor model in mice. Substantial tumor volume, tumor weight, and % inhibition of the tumor weight in treated and untreated groups were evaluated. In addition to the investigation of antitumor activity, the possible immunomodulatory activity was also assessed.
Results: The studies showed that the ALCM demonstrated more significant antioxidant activity. The results also showed more immunomodulatory activity when compared to AQCM.
Conclusion: The study proves that the plant extract is of higher therapeutic efficacy in cancer. However, the extracts require more exploration toward its usefulness in drug discovery.
EFFECTS OF ETHANOLIC EXTRACT OF RHINACANTHUS NASUTUS (L) KURZ AS AN ANTIMICROBIAL AGAINST ESCHERICHIA COLI BACTERIA USING IN VITRO METHOD
Authors: SUNARTI M.BIOMED  , DEBORA PANINSARI  
Published: December 2019

Objective: The objective of this study was to discover of the ethanolic extract of Rhinacanthus nasutus (L) Kurz in inhibiting Escherichia coli bacteria using an in vitro method.
Methods: This is an experimental study using a laboratory test with Kirby-Bauer or paper disc method by observing and measuring the inhibition zone of the ethanolic extract of R. nasutus against E. coli bacteria with extract concentrations of 15%, 30%, and 60% consisting of control groups and treatment group. The positive control group used chloramphenicol antibiotics and negative control groups used Aquadest. E. coli was incubated at 37°C for 24 h. Then, the plates were incubated for 24 h at 37°C and the diameter of the inhibition zone was observed until the 3rd day with three repetitions.
Results: The results of the study showed that the mean inhibition zone of E. coli bacteria was 10.93 mm, 12.09 mm, and 18.90 mm. The results of the Shapiro–Wilk test were p=0.199. The results of the one-way analysis of variance test were p<0.05 and that of the post hoc test indicated a significant value of p<0.05. Based on the results of the research, there were significant differences in the inhibition zone between the control group and the treatment group at a concentration of 15%, 30%, and 60%.
Conclusion: R. nasutus extract was effective to inhibit the growth of E. coli bacteria at concentrations of 15%, 30%, and 60%, so R. nasutus is effective as an antimicrobial.
ADHERENCE TO INSULIN IN SINGAPOREAN PEDIATRIC TYPE 1 DIABETES PATIENTS AND ITS IMPACT ON GLYCEMIC CONTROL AND HEALTH-CARE UTILIZATION
Authors: BRANDON WEN BING CHUA  , XIN YAN LIM  , KAR MEN POH  , JAMIE STEPHANIE CALEB  , MCVIN HUA HENG CHEEN  , SOO TING LIM  , NGEE LEK  
Published: December 2019

Objective: Optimizing glycemic control is challenging with insulin non-adherence. This study aimed to characterize the prevalence of non-adherence among Singaporean pediatric patients with type 1 diabetes mellitus (T1DM) and investigate its associated outcomes.
Methods: Singaporean patients with T1DM aged ≤18 years old with ≥1 year of insulin prescription between 2012 and 2016 were included in this retrospective, single-center longitudinal study. Patients on insulin pumps were excluded from the study. Non-adherence was defined as medication possession ratio (MPR) <100%. Glycemic control was defined using mean hemoglobin A1c (HbA1c) within the study period. Health-care utilization was defined as the number of outpatients, inpatient, and emergency visits. The t-test, Chi-square test, logistic regression, and Poisson regression were used to analyze means, proportions, factors associated with non-adherence, and association of non-adherence and health-care utilization, respectively. Sensitivity analyses were performed for MPR thresholds of 80% and 95%.
Results: A total of 206 patients were included in this study. Non-adherent patients were older, had a longer duration of diabetes since diagnosis and shorter duration of follow-up. Gender, race, financial class, and number of concurrent medications were comparable between groups. The prevalence of non-adherence was 34.0% (95% confidence interval [CI]: 27.9–40.7%). Non-adherent patients had a higher average HbA1c (non-adherent: 9.6% [2.1] vs. adherent: 8.6% [1.3], p<0.001). Non-adherence was not associated with health-care utilization. Patients with >5 years of diabetes were more likely to be non-adherent.
Conclusion: Non-adherence defined as MPR <100% is associated with poorer glycemic control. Further interventions may focus on patients with >5 years of diabetes to improve their adherence to insulin therapy.
MOLECULAR FRACTIONATION WITH CONJUGATE CAPS STUDY OF THE INTERACTION OF THE ANACARDIC ACID WITH THE ACTIVE SITE OF TRYPANOSOMA CRUZI GAPDH ENZYME: A QUANTUM INVESTIGATION
Authors: MÁRCIA MACHADO MARINHO  , RICARDO PIRES DOS SANTOS  , EVELINE MATIAS BEZERRA  , RONER FERREIRA COSTA  , CIRO SIQUEIRA FIGUEIRA  , ALICE MARIA COSTA MARTINS  , PEDRO LIMA NETO  , EMMANUEL SILVA MARINHO  , VALDER NOGUEIRA FREIRE  , EUDENILSON LINS ALBUQUERQUE  
Published: December 2019

Objective: The objective of this study was to use the molecular fractionation with conjugate caps (MFCC) method to elucidate the possible interaction mechanism of anacardic acid (AA) with the saturated alkyl chain (AA0) in the Trypanosoma cruzi glyceraldehyde-3-phosphate dehydrogenase (TcGAPHD) enzyme.
Methods: Initially, the geometry optimization of the AA three-dimensional structure (with the pentadecyl chain) was performed using density functional theory (B3LYP) calculations. With the AA0 optimization data, it was possible to plot the molecular electrostatic potential (MESP) surface. Molecular docking simulation was performed using automated coupling with the AutoDock Vina program. The best-fit conformation in the docking simulation of AA0 is the binding site used for the construction of the TcGAPHD-AA0 complex. Interaction energies between the AA0 molecule and the amino acid residues of the TcGAPHD enzyme were estimated using the MFCC strategy.
Results: To obtain more reliable quantitative information on the interaction of AA with the active site of the TcGAPHD enzyme, the fragmentation method was combined with conjugated layers (MFCC) and molecular docking. It can be observed that the AA0 molecule occupies a region near the active site of the chalepin molecule in the TcGAPHD enzyme, and the Ile13 residue has the strongest binding energy of approximately 25 kcal/mol with AA0, through a strong Van der Waals interaction.
Conclusion: The paper presents an improved quantitative analysis approach for assessing the contribution of individual amino acids to the free energy of interaction between AA and TcGAPHD. Specifically, the paper illustrates the advantageous approach of combining molecular docking with the MFCC method.
THE COMBINED EFFECT OF DEXAMETHASONE AND BACILLUS CALMETTE–GUÉRIN ON THE NEUROBEHAVIORAL ASPECT IN MALE MICE (MUS MUSCULUS)
Authors: ABDELKRIM HAOULI  , SAMIR DJEMLI  , ABDELKRIM TAHRAOUI  
Published: December 2019

Objective: Our study aims to evaluate the combined effect of Bacille Calmette–Guérin (BCG) and dexamethasone (DEX) on male mice undergoing behavioral testing (forced swimming test, elevated plus maze, tail suspension test, and sucrose intake). An intraperitoneal injection of 0.2 ml/mg of BCG and 0.1 ml/mg of DEX body weight was administered after all behavioral tests were applied.
Methods: This study is based on the distribution of 15 male mice in three groups: Controls, DEX, and DEX+BCG. These mice underwent behavioral tests.
Results: The result after administration of DEX and BCG was found after 10 days. We observed an increase in locomotors activity with some degree of anxiety.
Conclusion: This allowed us to conclude that the combination of DEX+BCG seems to play a depressinogenic effect that will develop to become antidepressive.
THE THE TERATOGENIC EFFECTS OF ETHANOLIC EXTRACT OF BINTANGUR LEAVES (CALOPHYLLUM SOULATTRI BURM. F) ON FEMALE WHITE RATS
Authors: INARAH FAJRIATY  , HAFRIZAL RIZA  , FAJAR NUGRAHA  , FRENGKI FRIANTO  
Published: December 2019

Objectives: Drugs can cause undesired effects on the fetus during pregnancy, especially embryonic/organogenesis which could lead to defects in the fetus because some types of drugs can penetrate the placenta and will undergo biotransformation into a highly reactive compound that has the potential to become a teratogenic compound. The aim of this research was to examine the teratogenic effect of bintangur leaves (Calophyllum soulattri Burm. F) ethanol extract to Sprague Dawley strain white rats.
Methods: The white rats are divided into four treatment groups: Control group was given carboxymethyl cellulose Na 1%, comparison group was given trimethoprim 360 mg/kg BW, C. soulattri leaves ethanol extract (CLE) 100 mg/kg BW, and CLE 500 mg/kg BW. The treatment was administrated since organogenesis period. Cesarian section was performed to pregnant rat at the 20th day to separate the fetuses. Observation covered body weight of pregnant rats, fetal biometric, morphological malformation, and skeletal formation.
Results: CLE 100 mg/kg BW and 500 mg/kg BW did not cause any change in the number of a living fetus, body weight, and length of fetuses like the comparison group. Both doses of CLE shown have a normal skeletal formation. Resorption was found in the comparison group and CLE 100 mg/kg BW with the percentage was 65.21% and 6.67%. It was found that there is no significant difference (p<0.05) between both doses of CLE compared to control group.
Conclusion: From the results, it is concluded that CLE did not have the teratogenic effect.
STUDIES ON GLUCOSE-LOWERING EFFICACY OF THE ACACIA SUMA ROXB. ROOT
Authors: JITENDRA DEBATA  , SUNDEEP KUMAR HK  
Published: December 2019

Objective: The objective of the study was to evaluate the glucose-lowering efficacy of the Acacia suma Roxb. (Family: Fabaceae) root extracts on Wister albino rats.
Methods: A. suma roots were shade dried, powdered, and extracted by Soxhlet extraction procedure using petroleum ether, chloroform, ethanol, and water. The acute toxicity studies were conducted on Swiss albino mice as per the OECD guidelines 423. The antidiabetic activity of extracts was evaluated on adult Wistar rats at dose levels of 100,200, and 400 mg/kg P.O, respectively each using normoglycemic,glucose-loaded and Streptozotocin-induced rats. Metformin (50 mg/kg) was used as a reference standard for activity comparison.
Results: Among the tested extracts, the ethanol extract was found to produce promising results that are comparable to that of the reference standard metformin.
Conclusion: The study established the scientific basis for the utility of this plant in the treatment of diabetes and justifies the use of the root of the plant for treating diabetes as suggested in folklore remedies.