bluuebunny/crossref_metadata_2025_split huggingface dataset:
Use of mouse-tracking software to detect faking-good behavior on personality questionnaires: an explorative study
Authors: Mazza Cristina , Monaro Merylin , Burla Franco , Colasanti Marco , Orrù Graziella , Ferracuti Stefano , Roma Paolo 
Published: March 2020

AbstractThe aim of the present study was to explore whether kinematic indicators could improve the detection of subjects demonstrating faking-good behaviour when responding to personality questionnaires. One hundred and twenty volunteers were randomly assigned to one of four experimental groups (honest unspeeded, faking-good unspeeded, honest speeded, and faking-good speeded). Participants were asked to respond to the MMPI-2 underreporting scales (L, K, S) and the PPI-R Virtuous Responding (VR) scale using a computer mouse. The collected data included T-point scores on the L, K, S, and VR scales; response times on these scales; and several temporal and spatial mouse parameters. These data were used to investigate the presence of significant differences between the two manipulated variables (honest vs. faking-good; speeded vs. unspeeded). The results demonstrated that T-scores were significantly higher in the faking-good condition relative to the honest condition; however, faking-good and honest respondents showed no statistically significant differences between the speeded and unspeeded conditions. Concerning temporal and spatial kinematic parameters, we observed mixed results for different scales and further investigations are required. The most consistent finding, albeit with small observed effects, regards the L scale, in which faking-good respondents took longer to respond to stimuli and outlined wider mouse trajectories to arrive at the given response.
Transcriptomic identification of miR-205 target genes potentially involved in metastasis and survival of cutaneous malignant melanoma
Authors: Sánchez-Sendra Beatriz , Serna Eva , Navarro Lara , González-Muñoz Jose F. , Portero Jesica , Ramos Alberto , Murgui Amelia , Monteagudo Carlos 
Published: March 2020

AbstractCutaneous melanoma is an aggressive neoplasm and is responsible for the majority of skin cancer deaths. Several miRNAs are involved in melanoma tumor progression. One of them is miR-205, the loss of which contributes to the development of melanoma metastasis. We evaluated whole-genome mRNA expression profiling associated with different miR-205 expression levels in melanoma cells. Differential expression analysis identified 243 differentially expressed transcripts including inositol polyphosphate 5′-phosphatase-like protein-1 (INPPL1) and BTB/POZ Domain-Containing Protein 3 (BTBD3). INPPL1 and BTBD3 were downregulated when melanoma cells expressed miR-205, indicating that these genes are potential miR-205 targets. Additionally, the target prediction algorithm TargetScan revealed that INPPL1 and BTBD3 genes had predicted target sites of miR-205 in their 3′UTRs and functional analysis demonstrated that these genes were directly linked to miR-205. Interestingly, our clinical data showed that INPPL1 was significantly associated with lymph node metastasis-free survival (LNMFS), distant metastasis-free survival (DMFS) and melanoma specific survival (MSS). This study supports INPPL1 as a miR-205 target gene and, therefore, that the involvement of miR-205 in the metastatic dissemination of malignant melanoma is, at least in part, via INPPL1.
Rhythmic expression of the melatonergic biosynthetic pathway and its differential modulation in vitro by LPS and IL10 in bone marrow and spleen
Authors: Córdoba-Moreno Marlina O. , de Souza Ewerton da Silva , Quiles Caroline L. , dos Santos-Silva Débora , Kinker Gabriela S. , Muxel Sandra M. , Markus Regina P. , Fernandes Pedro A. 
Published: March 2020

AbstractDaily oscillation of the immune system follows the central biological clock outputs control such as melatonin produced by the pineal gland. Despite the literature showing that melatonin is also synthesized by macrophages and T lymphocytes, no information is available regarding the temporal profile of the melatonergic system of immune cells and organs in steady-state. Here, the expression of the enzymes arylalkylamine-N-acetyltransferase (AA-NAT), its phosphorylated form (P-AA-NAT) and acetylserotonin-O-methyltransferase (ASMT) were evaluated in phagocytes and T cells of the bone marrow (BM) and spleen. We also determined how the melatonergic system of these cells is modulated by LPS and the cytokine IL-10. The expression of the melatonergic enzymes showed daily rhythms in BM and spleen cells. Melatonin rhythm in the BM, but not in the spleen, follows P-AA-NAT daily variation. In BM cells, LPS and IL10 induced an increase in melatonin levels associated with the increased expressions of P-AA-NAT and ASMT. In spleen cells, LPS induced an increase in the expression of P-AA-NAT but not of melatonin. Conversely, IL10 induced a significant increase in melatonin production associated with increased AA-NAT/P-AA-NAT expressions. In conclusion, BM and spleen cells present different profiles of circadian production of local melatonin and responses to immune signals.
Tethered primary hepatocyte spheroids on polystyrene multi-well plates for high-throughput drug safety testing
Authors: Tasnim Farah , Singh Nisha Hari , Tan Elijah Keng Foo , Xing Jiangwa , Li Huan , Hissette Sebastien , Manesh Sravanthy , Fulwood Justina , Gupta Kapish , Ng Chan Way , Xu Shuoyu , Hill Jeffrey , Yu Hanry 
Published: March 2020

AbstractHepatocyte spheroids are useful models for mimicking liver phenotypes in vitro because of their three-dimensionality. However, the lack of a biomaterial platform which allows the facile manipulation of spheroid cultures on a large scale severely limits their application in automated high-throughput drug safety testing. In addition, there is not yet a robust way of controlling spheroid size, homogeneity and integrity during extended culture. This work addresses these bottlenecks to the automation of hepatocyte spheroid culture by tethering 3D hepatocyte spheroids directly onto surface-modified polystyrene (PS) multi-well plates. However, polystyrene surfaces are inert toward functionalization, and this makes the uniform conjugation of bioactive ligands very challenging. Surface modification of polystyrene well plates is achieved herein using a three-step sequence, resulting in a homogeneous distribution of bioactive RGD and galactose ligands required for spheroid tethering and formation. Importantly, treatment of polystyrene tethered spheroids with vehicle and paradigm hepatotoxicant (chlorpromazine) treatment using an automated liquid handling platform shows low signal deviation, intact 3D spheroidal morphology and Z’ values above 0.5, and hence confirming their amenability to high-throughput automation. Functional analyses performance (i.e. urea and albumin production, cytochrome P450 activity and induction studies) of the polystyrene tethered spheroids reveal significant improvements over hepatocytes cultured as collagen monolayers. This is the first demonstration of automated hepatotoxicant treatment on functional 3D hepatocyte spheroids tethered directly on polystyrene multi-well plates, and will serve as an important advancement in the application of 3D tethered spheroid models to high throughput drug screening.
Prevalence and new onset of depression and anxiety among participants with AMD in a European cohort
Authors: Rezapour Jasmin , Schuster Alexander K. , Nickels Stefan , Korb Christina A. , Elbaz Hisham , Peto Tunde , Michal Matthias , Münzel Thomas , Wild Philipp S. , König Jochem , Lackner Karl , Schulz Andreas , Pfeiffer Norbert , Beutel Manfred E. 
Published: March 2020

AbstractTo investigate the prevalence and new onset of depression and anxiety among subjects with age-related macular degeneration (AMD) and its association with AMD in a large European cohort with relatively good visual acuity. 11,834 participants enrolled in the German population-based Gutenberg Health Study were studied. AMD was diagnosed by grading of fundus photographs. Depression and anxiety were assessed with the Patient Health Questionnaire and the Generalized Anxiety Disorder-2 Scale, respectively. Logistic regression analyses were performed and adjusted for several parameters. 1,089 (9.2%) participants were diagnosed having AMD. Prevalence of depression in AMD and non-AMD participants was 7.2% and 8.0%, respectively and prevalence of anxiety was 4.2% and 7.0%, respectively. New onset of depression and anxiety at 5-year follow-up in AMD subjects was 2.6% and 3.6%, respectively. AMD was not associated with depression (OR 0.93; CI 95% 0.70–1.20; p = 0.62). AMD was associated with less anxiety (OR 0.67; CI 95% 0.47–0.93; p = 0.02). This is the first study analyzing both prevalence and new onset of depression and anxiety in AMD subjects. AMD- and non-AMD participants had a similar prevalence and new onset of depression in our population-based sample. Participants without AMD had a higher prevalence of anxiety. AMD was not associated with depression.
Identifying vital nodes based on reverse greedy method
Authors: Ren Tao , Li Zhe , Qi Yi , Zhang Yixin , Liu Simiao , Xu Yanjie , Zhou Tao 
Published: March 2020

AbstractThe identification of vital nodes that maintain the network connectivity is a long-standing challenge in network science. In this paper, we propose a so-called reverse greedy method where the least important nodes are preferentially chosen to make the size of the largest component in the corresponding induced subgraph as small as possible. Accordingly, the nodes being chosen later are more important in maintaining the connectivity. Empirical analyses on eighteen real networks show that the reverse greedy method performs remarkably better than well-known state-of-the-art methods.
Calcium mishandling in absence of primary mitochondrial dysfunction drives cellular pathology in Wolfram Syndrome
Authors: La Morgia Chiara , Maresca Alessandra , Amore Giulia , Gramegna Laura Ludovica , Carbonelli Michele , Scimonelli Emanuela , Danese Alberto , Patergnani Simone , Caporali Leonardo , Tagliavini Francesca , Del Dotto Valentina , Capristo Mariantonietta , Sadun Federico , Barboni Piero , Savini Giacomo , Evangelisti Stefania , Bianchini Claudio , Valentino Maria Lucia , Liguori Rocco , Tonon Caterina , Giorgi Carlotta , Pinton Paolo , Lodi Raffaele , Carelli Valerio 
Published: March 2020

AbstractWolfram syndrome (WS) is a recessive multisystem disorder defined by the association of diabetes mellitus and optic atrophy, reminiscent of mitochondrial diseases. The role played by mitochondria remains elusive, with contradictory results on the occurrence of mitochondrial dysfunction. We evaluated 13 recessive WS patients by deep clinical phenotyping, including optical coherence tomography (OCT), serum lactic acid at rest and after standardized exercise, brain Magnetic Resonance Imaging, and brain and muscle Magnetic Resonance Spectroscopy (MRS). Finally, we investigated mitochondrial bioenergetics, network morphology, and calcium handling in patient-derived fibroblasts. Our results do not support a primary mitochondrial dysfunction in WS patients, as suggested by MRS studies, OCT pattern of retinal nerve fiber layer loss, and, in fibroblasts, by mitochondrial bioenergetics and network morphology results. However, we clearly found calcium mishandling between endoplasmic reticulum (ER) and mitochondria, which, under specific metabolic conditions of increased energy requirements and in selected tissue or cell types, may turn into a secondary mitochondrial dysfunction. Critically, we showed that Wolframin (WFS1) protein is enriched at mitochondrial-associated ER membranes and that in patient-derived fibroblasts WFS1 protein is completely absent. These findings support a loss-of-function pathogenic mechanism for missense mutations in WFS1, ultimately leading to defective calcium influx within mitochondria.
microRNA-670 modulates Igf2bp1 expression to regulate RNA methylation in parthenogenetic mouse embryonic development
Authors: Hao Jindong , Hu Haobo , Jiang Ziping , Yu Xianfeng , Li Chengshun , Chen Lin , Xia Yidan , Liu Da , Wang Dongxu 
Published: March 2020

AbstractAberrant epigenetic modification, including N6-methylation of adenosine (m6A), has been frequently reported in embryos derived from parthenogenetic activation (PA). However, the role of Igf2bp1 expression pattern in m6A modification and the mechanism through which Igf2bp1 function is regulated in PA embryos remains elusive. Therefore, in this study, using si-Igf2bp1 and betaine (N,N,N-trimethylglycine, a major methyl donor), we investigated the effect of Igf2bp1 expression in m6A modification on the development of PA embryos. The results indicated that the down-regulation of Igf2bp1 reduced the cleavage and blastula rates of PA embryos. Moreover, m6A expression level was markedly down-regulated following microinjection with si-Igf2bp1. However, the treatment with betaine could significantly restore the m6A level. Further bioinformatics analysis revealed Igf2bp1 as the putative target of microRNA 670 (miR-670). Thus, to confirm this finding, mimics and inhibitor of miR-670 were microinjected into PA embryos. The results demonstrated that miR-670 inhibitor augmented the expression of Igf2bp1 and rescued cleavage and blastula rates. In addition, the miR-670 inhibitor promoted the m6A expression level. TUNEL assay revealed a loss of expression of Igf2bp1 induced cell apoptosis in PA embryos. Taken together, these results demonstrated that miR-670-3p functions as the regulator of Igf2bp1 expression and plays a crucial role in PA development through m6A modification.
In vivo dynamic analysis of BMP-2-induced ectopic bone formation
Authors: Hashimoto Kunihiko , Kaito Takashi , Furuya Masayuki , Seno Shigeto , Okuzaki Daisuke , Kikuta Junichi , Tsukazaki Hiroyuki , Matsuda Hideo , Yoshikawa Hideki , Ishii Masaru 
Published: March 2020

AbstractBone morphogenetic protein (BMP)-2 plays a central role in bone-tissue engineering because of its potent bone-induction ability. However, the process of BMP-induced bone formation in vivo remains poorly elucidated. Here, we aimed to establish a method for intravital imaging of the entire process of BMP-2-induced ectopic bone formation. Using multicolor intravital imaging in transgenic mice, we visualized the spatiotemporal process of bone induction, including appearance and motility of osteoblasts and osteoclasts, angiogenesis, collagen-fiber formation, and bone-mineral deposition. Furthermore, we investigated how PTH1-34 affects BMP-2-induced bone formation, which revealed that PTH1-34 administration accelerated differentiation and increased the motility of osteoblasts, whereas it decreased morphological changes in osteoclasts. This is the first report on visualization of the entire process of BMP-2-induced bone formation using intravital imaging techniques, which, we believe, will contribute to our understanding of ectopic bone formation and provide new parameters for evaluating bone-forming activity.
PBRM1 mutation and preliminary response to immune checkpoint blockade treatment in non-small cell lung cancer
Authors: Zhou Huaqiang , Liu Jiaqing , Zhang Yaxiong , Huang Yan , Shen Jiayi , Yang Yunpeng , Fang Wenfeng , Zhang Li 
Published: March 2020

AbstractPolybromo-1 (PBRM1) gene is a promising biomarker for immunotherapy in clear cell renal cell carcinoma. But to our knowledge, the frequency and clinical relevance of PBRM1 mutation in lung cancer remain unknown. We conducted a retrospective study to evaluate the prevalence of PBRM1 mutation and its correlation with preliminary response to immunotherapy in non-small cell lung cancer (NSCLC). Our results indicated that PBRM1 mutation was more likely to be a negative predictive biomarker for immunotherapy in NSCLC.
Giant fractional Shapiro steps in anisotropic Josephson junction arrays
Authors: Panghotra R. , Raes B. , de Souza Silva Clécio C. , Cools I. , Keijers W. , Scheerder J. E. , Moshchalkov V. V. , Van de Vondel J. 
Published: March 2020

AbstractGiant fractional Shapiro steps have been observed in Josephson junction arrays as resulting from magnetic flux quantization in the two-dimensional array. We demonstrate experimentally the appearance of giant fractional Shapiro steps in anisotropic Josephson junction arrays as unambiguous evidence of a skewed current phase relationship. Introducing anisotropy in the array results in a giant collective high frequency response that reflects the properties of a single junction, as evidenced by the observation of a Fraunhofer like magnetic field dependence of the total critical current of the system. The observed phase dynamics can be perfectly captured within an extended resistively shunted Josephson junction model. These results directly indicate the potential of Josephson junction arrays to explore the current phase relation in a very broad frequency range (down to 50 MHz) and in a wide variety of novel link materials exhibiting non-conventional current phase relationships.
Preoperative systemic immune-inflammation index predicts prognosis and guides clinical treatment in patients with non-small cell lung cancer
Authors: Yan Xue , Li Guowei 
Published: March 2020

Abstract
Objectives: The purpose of the present study was to evaluate the prognostic value of a systemic immune-inflammation index (SII) and the relationship between SII and the effectiveness of postoperative treatment in patients with non-small cell lung cancer (NSCLC).
Methods: A total of 538 patients diagnosed with NSCLC who had undergone curative surgery were retrospectively enrolled in the study. Clinicopathologic and laboratory variables were collected. SII was defined as neutrophil × platelet/lymphocyte counts. Both univariate and multivariate analyses were performed to analyze the prognostic value of these factors.
Results: The preoperative SII level was associated with sex, smoking history, histological type, lesion type, resection type, pathological stage, neutrophil/lymphocyte ratio (NLR), platelet/lymphocyte ratio (PLR), lymphocyte/monocyte ratio (LMR), fibrinogen and bone metastasis (P&lt;0.05). The univariate and multivariate analyses revealed that SII was an independent prognostic factor for disease-free survival (DFS, P=0.033) and overall survival (OS, P=0.020). Furthermore, the prognostic value of SII was also verified regardless of the histological type and pathological stage. The subgroup analysis demonstrated that patients with a high SII may benefit from adjuvant therapy (P=0.024 for DFS and P=0.012 for OS).
Conclusion: An increased preoperative SII may independently predict the poor DFS and OS in patients with resectable NSCLC. SII may help select NSCLC patients who might benefit from adjuvant chemotherapy.
Modelling hystereses observed during dwarf nova outbursts
Authors: Hameury J.-M. , Knigge C. , Lasota J.-P. , Hambsch F.-J. , James R. 
Published: March 2020

Context. Although the disc instability model is widely accepted as the explanation for dwarf nova outbursts, it is still necessary to compare its predictions to observations because many of the constraints on angular momentum transport in accretion discs are derived from the application of this model to real systems.
Aims. We test the predictions of the model concerning the multicolour time evolution of outbursts for two well-observed systems, SS Cyg and VW Hyi.
Methods. We calculate the multicolour evolution of dwarf nova outbursts using the disc instability model and taking into account the contribution from the irradiated secondary, the white dwarf and the hot spot.
Results. Observations definitely show the existence of a hysteresis in the optical colour–magnitude diagram during the evolution of dwarf nova outbursts. We find that the disc instability model naturally explains the existence and the orientation of this hysteresis. For the specific cases of SS Cyg and VW Hyi, the colour and magnitude ranges covered during the evolution of the system are in reasonable agreement with observations. However, the observed colours are bluer than observed near the peak of the outbursts, as in steady systems, and the amplitude of the hysteresis cycle is smaller than observed. The predicted colours significantly depend on the assumptions made for calculating the disc spectrum during rise, and on the magnitude of the secondary irradiation for the decaying part of the outburst.
Conclusions. Improvements in the spectral disc models are strongly needed if the system evolution in the UV is to be addressed.
Qualität der Therapie von
Speicheldrüsenerkrankungen
Authors: Guntinas-Lichius Orlando 
Published: March 2020

ZusammenfassungDie Behandlung von Speicheldrüsenerkrankungen stellt ein wichtiges
Segment der HNO-Heilkunde dar. Von der Vielzahl von
Speicheldrüsenerkrankungen sieht der einzelne HNO-Arzt allerdings
möglicherweise nur wenige Fälle pro Jahr und chirurgische oder
endoskopisch minimal-invasive Therapiekonzepte spielen eine wichtige Rolle.
Daher kann der Erkenntnisgewinn nicht nur von prospektiven klinischen Studien
getragen werden, sondern heute im Wesentlichen durch Meta-Analysen und
potentiell auch von Registerdaten. Während eine Vielzahl guter
Indikatoren vorhanden ist, um die Funktion der erkrankten Speicheldrüse
und deren Besserung unter Therapie zu messen, sind Patient-reported outcome
measures (PROMs) bislang nicht ausreichend entwickelt worden. Es ist zu
fordern, dass diese Indikatoren auch konsequent in klinischen Studien eingesetzt
werden. Perspektivisch könnten dieselben Indikatoren auch für
Qualitätskontrollen in der ambulanten und stationären Routine
angewandt werden. Die Rahmenbedingungen für einen qualitativ
hochwertigen Wissenserwerb sind durch die HNO-Facharzt-Ausbildung, die
Verpflichtung der Weiterbildung und durch zertifizierte
Speicheldrüsen-Kurse prinzipiell sehr gut, die Vorgaben für
spezifische Qualitätsstandards für die Behandlung von Patienten
sind jedoch wenig ausgearbeitet. In Abgrenzung zu anderen Fachgebieten, die sich
mit Speicheldrüsenerkrankungen beschäftigen, sollte die
HNO-Heilkunde Standards mit hoher Qualitätsforderung für die
Behandlung von Patienten mit Speicheldrüsenerkrankungen entwickeln.
Qualitätsmanagement in der HNO – eine
Standortbestimmung
Authors: Wallner Frank 
Published: March 2020

ZusammenfassungUm mit der Unvollkommenheit ärztlichen Handelns umzugehen, gibt es neben
gesellschaftlichen Sanktionen schon seit der Antike ethische Verhaltensvorgaben
für Ärzte, die unser heutiges Verständnis von einem guten Arzt
geformt haben. Mit Industrialisierung und Standardisierung wurde vor 100 Jahren in den USA
der Grundstein für Qualitätsmanagement in der Medizin gelegt. In den
1950ern entstand in Japan ein umfassendes Qualitätskonzept, dass großen
Einfluss auf die Medizin gewinnen sollte. Alle heutigen Zertifizierungs- und
Akkreditierungsverfahren gehen aus diesen Wurzeln hervor.Seit 15 Jahren knüpft der Gesetzgeber in Deutschland ein immer feineres Netz an
Regularien, um durch externe Qualitätssicherung Mindeststandards im
Gesundheitswesen zu gewährleisten. Ärztliche Institutionen der
Selbstverwaltung verbessern darüber hinaus durch eigene Initiativen die
Behandlungsqualität.Qualitätsmanagement hat dauerhaft Einzug gehalten in Krankenhäuser und
Praxen, zu erkennen an Risikomanagement und Patientenorientierung. Daneben gibt es eine
Vielzahl an freiwilligen ärztlichen Initiativen zur Qualitätssicherung,
z. B. die Leitlinien der ärztlichen Fachgesellschaften.Per Umfrage wurde die Implementation eines Qualitätsmanagementsystems in
deutschen HNO-Kliniken und -Praxen evaluiert und mit dem Bundesdurchschnitt verglichen. Es
fand sich überwiegend eine umfassendere Umsetzung als in den
Vergleichsgruppen.Die aktuellen Herausforderungen an Kliniken und Praxen sind erheblich und haben
Auswirkungen auf das Qualitätsniveau. Unterfinanzierung, Personalmangel und
gesellschaftliche Veränderungen sind hier genauso relevant wie Auswirkungen des
wissenschaftlichen Fortschritts.Um Qualitätsmanagement zukünftig umfassend zu implementieren, sind kluge
politische Weichenstellungen und Ressourcen entscheidend – das Konzept an sich hat
sich längst durchgesetzt.