bluuebunny/crossref_metadata_2025_split huggingface dataset:
Maintaining the Sustainable Energy Systems: Turning from Cost to Value
Authors: Pysmenna U. Yе. , Trypolska G. S. 
Published: February 2020

The economies of the world are influenced by the rapidly changing global energy policy agenda. Understanding energy trends implications in the long-term perspective is crucial for responsible and informed sustainability-policy making, with respect to transformations required to enhance the security of energy supply, resource efficiency and affordability, as well to as transformations required to minimize energy poverty and mitigate ecological footprint. Nowadays the price (value) competitiveness of technologies and products as their ability to respond to sustainability demands is becoming the appreciable criterion in choosing the pathways of technological growth or economic strategies designing. The transition to energy sustainability is the so-called quiet energy [r]evolution, or the transition towards 100 % renewable energy supply. Using the sociotechnical transition, vulnerability and sustainable development theories for the assessment of the energy safety level, this article aims to contribute to the understanding of cultural, institutional and innovation prerequisites of sustainable energy transitions. Basing on historical examples, it argues that, despite the cultural dimensions, energy resources and energy mix disparity, geographic location and income per capita, the value instead of cost philosophy in choosing energy pathways maintains the sustainable energy transitions. The key findings are the defined prerequisites of energy transitions sustainability; among them there are cultural dimensions, innovations and the speeds of movement along learning curves when adopting new energy technologies as well as energy policy patterns, applied in a country: value versus cost-driven. The Value vs Cost Energy Policy matrix has been developed in order to determine if a country is sufficiently value-driven in its energy policy.
Influence of roughness of a landing aperture of a frame on quality of production of the drawing tool and process of wire drawing
Authors: Radzkova I. N. , Hapeyenka T. V. , Lazebnikova I. P. 
Published: February 2020

Relevance. Asymmetry of the drawing channel during processing and destruction of the drawing in the process of drawing.Purpose. To determine the effect of the parameter of roughness of the landing hole of frames on the quality of manufacturing of lugsThe results of the study. The influence of the roughness parameter of the bore rims on manufacturing quality dies was defined.
Vaccination Coverage with the Pneumococcal and Influenza Vaccine Among Persons with Chronic Diseases in Shanghai, China, 2017
Authors: Wang Yuheng , Cheng Minna , Wang Siyuan , Wu Fei , Yan Qinghua , Yang Qinping , Li Yanyun , Guo Xiang , Fu Chen , Shi Yan , Wagner Abram L. , Boulton Matthew L. 
Published: February 2020

Abstract
Background: Adults with chronic conditions such as heart disease, diabetes, or lung disease are more likely to develop complications from a number of vaccine-preventable diseases, including influenza and pneumonia. In this study, we use the data from a chronic disease management information system in Shanghai to estimate vaccination coverage and characterize predictors of seasonal influenza and 23-valent pneumococcal polysaccharide vaccine (PPSV23) vaccination among people with chronic disease in Shanghai.Methods: The Shanghai Centers for Disease Control and Prevention have information systems related to chronic disease management, hospital records, and immunizations. Data from individuals with hypertension, diabetes and chronic obstructive pulmonary disease (COPD) were abstracted during July 2017. The main outcome was coverage of pneumococcal and influenza vaccination. Vaccination coverage was calculated across demographic groups. Significance in bivariate associations was assessed through Pearson’s chi-square tests, and in multivariable models through logistic regression models with a forward stepwise method to select variables.Results: In the sample of 2,531,227 individuals ≥15 years, 22.8% were vaccinated for pneumonia from January 2013 to July 2017, and the vaccination coverage of influenza in the 2016/17 influenza season was 0.4%. Vaccination coverage was highest in those 70-79 and lowest in those younger than 60. Compared to urban areas, uptake in rural areas was higher for pneumonia vaccination (OR: 2.43, 95% CI: 2.41, 2.45), but lower for influenza vaccination (OR: 0.55, 95% CI: 0.51, 0.59). Having a greater number of chronic diseases was associated with higher likelihood of pneumonia vaccination (3 vs 1: OR: 1.68, 95% CI: 1.64, 1.71), but this relationship was not statistically significant for influenza vaccination.Conclusions: We found low levels with of pneumococcal vaccination, and extremely low uptake of influenza vaccination among individuals with high risk conditions in Shanghai who should be priority groups targeted for vaccination. Interventions could be designed to target groups with low uptake – like younger adults, and individuals who have not yet retired.
Seroprevalence of Brucella Infection and Associated Factors among Pregnant Women Receiving Antenatal Care around Human, Wildlife and Livestock Interface in Ngorongoro Ecosystem, Northern Tanzania. A Cross Sectional Study
Authors: Makala Robert , Majigo Mtebe Venance , Bwire George M. , Kibwana Upendo , Mirambo Mariam M. , Joachim Agricola 
Published: February 2020

Abstract
Background
Brucellosis is a zoonotic disease transmitted to humans through contact with infected animals, animal products or consumption of infected dairy products. Brucella infection during pregnancy is of special interest due to association with adverse pregnancy outcomes. This study determined the seroprevalence and factors associated with Brucella infection among pregnant women around the human-wildlife-livestock interface area in Ngorongoro Ecosystem, Northern Tanzania.
Methods
A facility-based cross-sectional study was conducted between May and June 2018 at six health facilities that provide antenatal services. Pregnant women receiving antenatal care were invited to participate. A structured questionnaire was used to collect socio-demographic and obstetric characteristics in addition to behavior and practices related to the occurrence of human brucellosis. The presence of serum immunoglobulin against Brucella was determined using Rose Bengal Plate Test (RBPT). The positive samples were further assayed for the presence of IgG and IgM using Enzyme-Linked Immunosorbent assay. Bivariate analysis was conducted to determine the variables associated with Brucella seropositivity. Multivariable logistic regression analysis was performed to examine the factors independently associations with Brucella seropositivity after adjustment for other explanatory variables.
Results
A total of 313 participants were enrolled in the study. The overall seroprevalence of Brucella infection was 10.9 % (34/313) determined by Rose Bengal plate test. Of 34 positive individuals, 27(79.4%) and 8(23.5%) were positive in the ELISA specific for IgG and IgM Brucella antibodies respectively. Regular contact with manure (AOR 3.16, 95%CI 1.27-7.83) and preference for animal fresh milk (AOR 3.80, 95 % CI 1.23–11.69), raw meat (AOR 2.58, 95 % CI 1.14 – 5.81) and raw animal blood (AOR 2.71, 95 % CI 1.15–6.35) increased the odds of being Brucella seropositive . Contact with the animal placenta were not associated with Brucella seropositivity after adjustment.
Conclusion
This study has found that brucellosis is an important public health problem among pregnant women in areas with interactions of humans; livestock and wildlife. The risk of infection increased with the regular contact with manure and preference of raw foodstuffs like animal blood, meat, and milk. We emphasize the need for interventional strategies to reduce the risk of exposure.
Robotic radical antegrade modular pancreatosplenectomy(RAMPS) versus standard retrograde pancreatosplenectomy (SRPS):study protocol for a randomized controlled trial
Authors: Zhang Gong , Liu Rong , Kang Yuhao , Zhang Haifeng , Wang Fei 
Published: February 2020

Abstract
Background There has been data from meta-analysis suggesting that RAMPS is a safe and effective procedure for adenocarcinoma in the body or tail of the pancreas and is oncologically superior to SRPS. However, previous studies on RAMPS were conducted under the open and laparoscopic surgery. Robotic surgery, on the other hand, plays a role in ergonomics and offers several advantages, including less fatigue, tremor filtering, 7° of wrist-like motion, motion scaling, and three-dimensional vision. At present, there is still a lack of clinical studies to observe the safety and clinical efficacy of Robotic RAMPS in the world. Hence,prospective randomized controlled trials (RCTs) comparing Robotic RAMPS and SRPS are required. We begin a RCT trial to compare short-term surgical and oncological outcomes of Robotic RAMPS and SRPS for patients with distal pancreatectomy.Methods This is a randomized, single-center clinical trial. All included adults are patients with primary carcinoma of the distal pancreatectomy. A total of 246 patients will be randomly allocated to Robotic RAMPS or SRPS. The primary endpoints are oncological outcomes(R0 rate, number of Lymph node). Secondary endpoints are the perioperative complications, Perioperative indicators(operative time,blood loss,blood transfusion volume,costs).Discussion To evaluate the surgical and oncological outcomes of Robotic RAMPS, we therefore undertake a prospective RCT. This procedure may become a standard approach to robotic pancreatosplenectomy.
Use of an Oxygen Planar Optode to Assess the Effect of High Velocity Microsprays on Oxygen Penetration in a Human Dental Biofilms In-Vitro
Authors: Khosravi Yalda , Kandukuri Raja Durga Prasad , Palmer Sara , Gloag Erin Samantha , Borisov Sergey M. , Starke Michelle , Ward Marilyn , Kumar Purnima , de Beer Dirk , Chennu Arjun , Stoodley Paul 
Published: February 2020

Abstract
Background Dental plaque biofilms are the causative agents of caries, gingivitis and periodontitis. Both mechanical and chemical strategies are used in routine oral hygiene strategies to reduce plaque build-up. If allowed to mature biofilms can create anoxic microenvironments leading to communities which harbor pathogenic Gram-negative anaerobes. When subjected to high velocity fluid jets and sprays biofilms can be fluidized which disrupts the biofilm structure and allows the more efficient delivery of antimicrobial agents. Methods To investigate how such jets may disrupt anoxic niches in the biofilm, we used planar optodes to measure the dissolved oxygen (DO) concentration at the base of in-vitro biofilms grown from human dental saliva and plaque. These biofilms were subject to “shooting” treatments with a commercial high velocity microspray (HVM) device. Results HVM treatment resulted in removal of much of the biofilm and a concurrent rapid shift from anoxic to oxic conditions at the base of the surrounding biofilm. We also assessed the impact of HVM treatment on the microbial community by tracking 7 target species by qRT-PCR. There was a general reduction in copy numbers of the universal 16S RNA by approximately 95%, and changes of individual species in the target region ranged from approximately 1 to 4 log reductions. Conclusion We concluded that high velocity microsprays removed a sufficient amount of biofilm to disrupt the anoxic region at the biofilm-surface interface.
The different dietary sugars modulate the composition of the gut microbiota in honeybee during overwintering
Authors: Wang Hongfang , Liu Chunlei , Liu Zhenguo , Wang Ying , Ma Lanting , Xu Baohua 
Published: February 2020

Abstract
Background: The health of honeybee colonies is critical for bee products and agricultural production, and colony health is closely associated with the bacteria in the guts of honeybees. Although colony loss in winter is now the primary restriction in beekeeping, the effects of different sugars as winter food on the health of honeybee colonies are not well understood. Therefore, in this study, the influence of different sugar diets on honeybee gut bacteria during overwintering was examined.
Results: The bacterial communities in honeybee midguts and hindguts before winter and after bees were fed honey, sucrose, and high-fructose syrup as winter-food were determined by targeting the V4 region of 16S rDNA using the Illumina MiSeq platform.
The dominant microbiota in honeybee guts were the phyla Proteobacteria (63.17%), Firmicutes (17.61%; Lactobacillus, 15.91%), Actinobacteria (4.06%; Bifidobacterium, 3.34%), and Bacteroidetes (1.72%). The dominant taxa were conserved and not affected by season, type of overwintering sugar, or spatial position in the gut. However, the relative abundance of the dominant taxa was affected by those factors. In the midgut, microbial diversity of the sucrose group was higher than that of the honey and high-fructose syrup groups, but in the hindgut, microbial diversity of the honey and high-fructose groups was higher than that in the sucrose group. Sucrose increased the relative abundance of Actinobacteria (Bifidobacteriales Bifidobacteriaceae) and Alphaproteobacteria (Rhizobiales and Mitochondria) of honeybee midgut, and honey enriched the Bacteroidetes and Gammaproteobacteria (Pasteurellales) in honeybee hindgut. High-fructose syrup increased the relative abundance of Betaproteobacteria (Neisseriales: Neisseriaceae) of the midgut.
Conclusion: The type of sugar used as winter food affected the relative abundance of the dominant bacterial communities in honeybee guts, not the taxa, which could affect the health and safety of honeybee colonies during overwintering. The presence of the supernal Alphaproteobacteria, Bifidobacteriales, and Lactobacillaceae in the gut of honeybees fed sucrose and cheaper than honey both indicate that sucrose is very suitable as the overwintering food for honeybees.
Keywords: Honeybees; Sugar diet; Gut bacteria; Microbial diversity; Overwintering
Analysis of structural variants in four African Cichlids highlights an association with developmental and immune related genes
Authors: Dolfin Luca Penso , Man Angela , Mehta Tarang , Haerty Wilfried , Palma Federica Di 
Published: February 2020

Abstract
Background . East African lake cichlids are one of the most impressive examples of an adaptive radiation. Independently in Lake Victoria, Tanganyika, and Malawi, several hundreds of species arose within the last 10 million to 100,000 years. Whereas most analyses in cichlids focused on nucleotide substitutions across species to investigate the genetic bases of this explosive radiation, to date, no study has investigated the contribution of structural variants (SVs) in the evolution of adaptive traits across the three Great Lakes of East Africa.
Results . Here, we annotate and characterize the repertoires and evolutionary potential of different SV classes (deletion, duplication, inversion, insertions and translocations) in four cichlid species: Haplochromis burtoni, Metriaclima zebra, Neolamprologus brichardi and Pundamilia nyererei . We investigate the patterns of gain and loss evolution for each SV type, enabling the identification of lineage specific events. Both deletions and inversions show a significant overlap with SINE elements, while inversions additionally show a limited, but significant association with DNA transposons. Inverted regions are enriched for genes regulating behaviour, or involved in skeletal and visual system development. We also find that duplicated regions show enrichment for genes associated with “antigen processing and presentation” and other immune related categories. Our pipeline and results were further tested by PCR validation of selected deletions and inversions, which confirmed respectively 7 out of 10 and 6 out of 9 events.
Conclusions . Altogether, we provide the first comprehensive overview of rearrangement evolution in East African cichlids, and some important insights into their likely contribution to adaptation.
Silencing of microRNA-210 inhibits the progression of liver cancer and Hepatitis B virus-associated liver cancer via targeting EGR3
Authors: Li Xiaojie , Yuan Mei , Song Lu , Wang Yan 
Published: February 2020

Abstract
Background: This study was aimed to investigate the regulatory role of microRNA-210 (miRNA-210) on the progression of liver cancer and Hepatitis B virus (HBV)-associated liver cancer. Methods: The expression of miRNA-210 was detected in liver tissues of HBV-associated cirrhosis and liver cancer, and in HepG2 and HepG2.2.15 cells by qRT-PCR. MiRNA-210 was silenced in HepG2 and HepG2.2.15 cells by the transfection of miRNA-210 inhibitor. The cell viability and apoptosis was detected by MTT assay and Annexin V-fluorescein isothiocyanate/propidium iodide staining, respectively. The protein expression of EGR3 was detected by Western blot. The regulatory relationship between EGR3 and miRNA-210 was predicted by TargetScan and identified by Dual luciferase reporter gene assay.Results: MiRNA-210 was overexpressed in the liver tissues of HBV-associated cirrhosis and liver cancer, and in HepG2 and HepG2.2.15 cells (P < 0.05). Silencing of miRNA-210 inhibited the viability and promoted the apoptosis of HepG2 and HepG2.2.15 cells (P < 0.05). EGR3 was a target of miRNA-210, which was down-regulated in the liver tissues of HBV-associated cirrhosis and liver cancer, and in HepG2 and HepG2.2.15 cells (P < 0.05). Silencing of miRNA-210 increased the mRNA and protein expression of EGR3 (P < 0.05). Silencing of EGR3 reversed the anti-tumor effect of miRNA-210 inhibitor on HepG2 and HepG2.2.15 cells (P < 0.05).Conclusions: Silencing of miRNA-210 inhibits the progression of liver cancer and HBV-associated liver cancer via up-regulating EGR3.
Effects of blended versus offline case-centered learning on academic performance and critical thinking ability among undergraduate nursing students：a cluster randomized controlled trial
Authors: Yu Zhenzhen , Shen Ling , Zhuang Jiayuan , Chen Yimin , Chen Meijing , Lin Yazhu , Hu Rong 
Published: February 2020

Abstract
Background: Educational reform, especially methods of teaching, has been a focus among nursing educators. This study explored the impact of blended versus offline case-centered learning on academic performance of Medical Nursing and the development of critical thinking among undergraduate nursing students.
Methods: A cluster randomized controlled study design was used, with assessments immediately before and one school year after the intervention. There were two classes for second-year undergraduate nursing students enrolled in the study. The two classes of Medical Nursing were randomly allocated to either the experimental class, which undertook blended case-centered learning, or the control class, which undertook offline case-centered learning. The primary outcomes were academic performance consisting of final exam and process assessment, as well as the critical thinking ability assessed with the Critical Thinking Disposition Inventory-Chinese Version (CTDI-CV). The Mann-Whitney U test and the unpaired t test was subsequently used. ANCOVA analyses were also performed to examine the two teaching methods’ effect on critical thinking.
Results: The median score in the Medical Nursing course was slightly higher in the experimental class than in the control class after 1 academic year, although was no significant difference in statistics. In addition, compared with the control class, the pre-post difference in competency in critical thinking self-confidence in the experimental class was significantly greater. In the experimental class, there was significant improvement compared with baseline in dimension of critical thinking self-confidence (p<0.05). In the control class, there was significant improvement compared with baseline in the total score (p<0.05) and two of the seven dimensions: truth-seeking (p<0.05) and systematicity (p<0.05).
Conclusions: Our study confirms the effectiveness of blended and offline learning (both based on case-centered learning) for academic performance and components of critical thinking among undergraduate nursing students. Blended and offline case-centered learning could be applied to other nursing subjects in future studies. Moreover, further efforts to improve teaching are warranted.Keywords: education, nursing, teaching methods, critical thinking
A pilot study: A possible implication of Candida as an etiologically endogenous pathogen for oral lichen planus
Authors: He Hong , Xia Xinyu , Yang Haiping , Peng Qiao , Zheng Jiaoer 
Published: February 2020

Abstract
Background: The aim of this study was to investigate the prevalence and genotypic profiles of Candida albicans in patients with oral lichen planus (OLP). Materials and Methods: Positive rates and genotypic profiles of Candida albicans strains from OLP patients and healthy controls were analyzed. Random amplified polymorphic DNA and internal transcribed spacer of ribosome DNA polymerase chain reactions were used to sequence the DNA of these strains, and then their genetic similarity was measured using BLAST, UIV Band, and Vector NTI Suite Sequence Analyses Software. Results: The prevalence of C. albicans strains detected from erosive-OLP, non-erosive OLP, and normal individuals was 18.87%, 18.75%, and 7.92%, respectively. Four different genotypes were revealed by the two methods. To be specific, type I was found only in the healthy subjects; type II a and II b were found in non-erosive OLP, and type III was identified in erosive OLP. Intragroup similarity coefficients, i.e. SAB were 100%, and inter-groups similarity coefficients, i.e. SAB were less than 30%. Conclusions: The genotypic results of C. albicans in OLP revealed an endogenous rather than exogenous infection of C. albicans. In addition, a possible pathogenic role of C. albicans in OLP, with the etiologic sense contributing to a more proper recognition on the pathogenesis, development, and progression of OLP, as well as some strategies for its diagnosis and treatment were identified.
Labial gingival thickness assessment at mandibular incisors of orthodontic patients with ultrasound and cone-beam ct. A cross-sectional study
Authors: Kloukos Dimitrios , Kakali Lydia , Koukos George , Sculean Anton , Stavropoulos Andreas , Katsaros Christos 
Published: February 2020

Abstract

Background Quantitative and qualitative analysis of several periodontal parameters plays an important role in several dental procedures. Aim of the current study was to assess gingival thickness (GT) at mandibular incisors of orthodontic patients with two methods and determine how these methods are compared to each other when assessing periodontal anatomy through soft tissue thickness. Methods The sample consisted of 40 consecutive adult orthodontic patients. GT was measured at both central mandibular incisors, mid-facially on the buccal aspect, 2mm apically to the free gingival margin with two methods: a) clinically with an Ultrasound device (USD) and b) radiographically with Cone Beam Computed Tomography (CBCT). Results CBCT measurements were consistently higher than USD measurements, with the difference ranging from 0.13 mm to 0.21 mm. No difference was noted between the repeated CBCT measurements at the right central incisor (Bias= 0.05 mm; 95% CI= -0.01, 0.11, p=0.104). Although the respective results for the left incisor indicated, statistically, that the measurements were not exactly replicated, the magnitude of the point estimate was small and not clinically significant (Bias= 0.06 mm; 95% CI= 0.01, 0.11, p=0.014). Small differences between CBCT measurements made by the 2 examiners at the left central incisor (bias= 0.06 mm, 95% CI= 0.01, 0.11, p=0.014) were detected. However, this difference was minor, and again, not clinically significant. The respective analysis on the right incisor showed no significant difference (bias= 0.05 mm, 95% CI= -0.01, 0.11, p=0.246). Conclusions Present data indicate that CBCT measurements were highly reproducible and yielded greater values compared to USD measurements.