bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prdm9Intersubspecific Interactions in Hybrid Male Sterility of House Mouse
Authors: Mukaj Amisa , Piálek Jaroslav , Fotopulosova Vladana , Morgan Andrew Parker , Odenthal-Hesse Linda , Parvanov Emil D , Forejt Jiri 
Published: July 2020

AbstractThe classical definition posits hybrid sterility as a phenomenon when two parental taxa each of which is fertile produce a hybrid that is sterile. The first hybrid sterility gene in vertebrates, Prdm9, coding for a histone methyltransferase, was identified in crosses between two laboratory mouse strains derived from Mus mus musculus and M. m. domesticus subspecies. The unique function of PRDM9 protein in the initiation of meiotic recombination led to the discovery of the basic molecular mechanism of hybrid sterility in laboratory crosses. However, the role of this protein as a component of reproductive barrier outside the laboratory model remained unclear. Here, we show that the Prdm9 allelic incompatibilities represent the primary cause of reduced fertility in intersubspecific hybrids between M. m. musculus and M. m. domesticus including 16 musculus and domesticus wild-derived strains. Disruption of fertility phenotypes correlated with the rate of failure of synapsis between homologous chromosomes in meiosis I and with early meiotic arrest. All phenotypes were restored to normal when the domesticus Prdm9dom2 allele was substituted with the Prdm9dom2H humanized variant. To conclude, our data show for the first time the male infertility of wild-derived musculus and domesticus subspecies F1 hybrids controlled by Prdm9 as the major hybrid sterility gene. The impairment of fertility surrogates, testes weight and sperm count, correlated with increasing difficulties of meiotic synapsis of homologous chromosomes and with meiotic arrest, which we suppose reflect the increasing asymmetry of PRDM9-dependent DNA double-strand breaks.
STing: accurate and ultrafast genomic profiling with exact sequence matches
Authors: Espitia-Navarro Hector F , Chande Aroon T , Nagar Shashwat D , Smith Heather , Jordan I King , Rishishwar Lavanya 
Published: July 2020

Abstract
Genome-enabled approaches to molecular epidemiology have become essential to public health agencies and the microbial research community. We developed the algorithm STing to provide turn-key solutions for molecular typing and gene detection directly from next generation sequence data of microbial pathogens. Our implementation of STing uses an innovative k-mer search strategy that eliminates the computational overhead associated with the time-consuming steps of quality control, assembly, and alignment, required by more traditional methods. We compared STing to six of the most widely used programs for genome-based molecular typing and demonstrate its ease of use, accuracy, speed and efficiency. STing shows superior accuracy and performance for standard multilocus sequence typing schemes, along with larger genome-scale typing schemes, and it enables rapid automated detection of antimicrobial resistance and virulence factor genes. STing determines the sequence type of traditional 7-gene MLST with 100% accuracy in less than 10 seconds per isolate. We hope that the adoption of STing will help to democratize microbial genomics and thereby maximize its benefit for public health.
Development of multiepitope subunit protein vaccines against Toxoplasma gondii using an immunoinformatics approach
Authors: Onile Olugbenga S , Ojo Glory J , Oyeyemi Bolaji Fatai , Agbowuro Gbenga O , Fadahunsi Adeyinka I 
Published: July 2020

Abstract
Approximately one-third of the world’s human population is estimated to have been exposed to the parasite Toxoplasma gondii. Its prevalence is reportedly high in Ethiopia (74.80%) and Zimbabwe (68.58%), and is 40.40% in Nigeria. The adverse effect of this parasite includes a serious congenital disease in the developing fetus of pregnant women. After several efforts to eliminate the disease, only one licensed vaccine ‘Toxovax’ has been used to avoid congenital infections in sheep. The vaccine has been adjudged expensive coupled with adverse effects and short shelf life. The potential of vaccine to likely revert to virulent strain is a major reason why it has not been found suitable for human use, hence the need for a vaccine that will induce T and B memory cells capable of eliciting longtime immunity against the infection. This study presents immunoinformatics approaches to design a T. gondii-oriented multiepitope subunit vaccine with focus on micronemal proteins for the vaccine construct. The designed vaccine was subjected to antigenicity, immunogenicity, allergenicity and physicochemical parameter analyses. A 657-amino acid multiepitope vaccine was designed with the antigenicity probability of 0.803. The vaccine construct was classified as stable, non-allergenic, and highly immunogenic, thereby indicating the safety of the vaccine construct for human use.
Synaptic input to brain tumors: clinical implications
Authors: Venkataramani Varun , Tanev Dimitar Ivanov , Kuner Thomas , Wick Wolfgang , Winkler Frank 
Published: July 2020

Abstract
The recent discovery of synaptic connections between neurons and brain tumor cells fundamentally challenges our understanding of gliomas and brain metastases and shows how these tumors can integrate into complex neuronal circuits. Here, we provide an overview of glutamatergic neuron-to-brain tumor synaptic communication (NBTSC) and explore novel therapeutic avenues. First, we summarize current concepts of direct synaptic interactions between presynaptic neurons and postsynaptic glioma cells, and indirect perisynaptic input to metastatic breast cancer cells. We explain how these novel structures drive brain tumor growth and invasion. Second, a vicious cycle of enhanced neuronal activity, including tumor-related epilepsy, and glioma progression is described. Finally, we discuss which future avenues to target NBTSC appear most promising. All in all, further characterization of NBTSC and the exploration of NBTSC-inhibiting therapies have the potential to reveal critical vulnerabilities of yet incurable brain tumors.
Current status and issues concerning Health Japan 21 (second term)
Authors: Tsuji Ichiro 
Published: July 2020

Abstract
The national health promotion program, Health Japan 21 (second term), began in 2013. This program has set 5 basic goals: to extend healthy life expectancy and reduce health disparity, to prevent noncommunicable disease, to improve function for social life, to establish a social environment for health, and to improve lifestyle. To achieve these goals, Health Japan 21 (second term) set 53 targets for the period between 2013 and 2022. At the interim evaluation in 2018, only 21 of the 53 targets were judged likely to be achieved by 2022. In addition, 18 targets were judged as unchanged, and 1 target was judged to be deteriorating. Thus, to achieve the goals of Health Japan 21 (second term), further efforts to strengthen health promotion programs across Japan are needed.
Social implementation of healthy diets
Authors: Kuwata Tamotsu ,   for Working Group 3 of the Healthy Diet Research Committee of International Life Sciences Institute, Japan
Published: July 2020

Abstract
Many issues regarding the social implementation of healthy diets remain to be solved. The structure of these issues and the route to achieve social implementation are not well organized. Working Group 3 of the Healthy Diet Research Committee of International Life Sciences Institute, Japan, surveyed some precedent cases aiming to establish a healthy diet and, by categorizing the success factors, developed recommendations for the social implementation of a healthy diet. Interviews were conducted with 8 “good health management” companies in various industries that target the “work area.” In the hearing, the Reach, Effectiveness, Adoption, Implementation, Maintenance model was used as a reference for evaluating the intervention effects. Many problems in implementation were revealed, especially with respect to continuity in and approaches to addressing indifference to health. “Management intention,” “Linking with their own business,” and “Visualization” were cited as factors for successful implementation of the health measures.
E-cigarettes: A new hazard for children and adolescents
Authors: Richmond Sarah A , Pike Ian , Maguire Jonathon L , Macpherson Alison 
Published: July 2020

AbstractObjectivesElectronic cigarettes and fluid (e-cigarettes, e-fluid) are hazardous materials that when inhaled or ingested may pose significant health risks to children and adolescents. The objective of this work was to explore the spectrum of injury related to e-cigarette exposure among Canadian children and adolescents.MethodsA one-time survey was sent to all paediatricians in Canada. Information was collected on children and adolescents who presented with e-cigarette exposure (inhalation and ingestion cases) in the previous 12 months. Questions included the number of injuries and symptoms, in addition to age, sex, treatment setting, intentional e-cigarette use, and how the products were accessed.ResultsA total of 520 surveys were completed and returned, identifying 35 cases. Symptoms related to inhalation were present in 30 cases and in 5 ingestion cases (5 unintentional, 0 intentional). For inhalation cases, most were male, ages 15 to 19 years, who sought treatment for nausea/vomiting, cough, throat irritation, or acute nicotine toxicity in an outpatient clinic/office. Most inhalation cases reported e-cigarette use 2 to 3 days/week, and e-cigarettes purchases from a mall kiosk/store. For ingestion cases, most were male, ages 1 to 4 years presenting to an emergency department with nausea/vomiting, cough, or respiratory irritation. Younger cases accessed e-fluid at home, older cases purchased in a mall kiosk/store. E-fluid flavours reported consumed were fruit, candy, and tobacco.ConclusionsE-cigarettes, recently introduced into the North American market are hazardous to children and adolescents. Given the low response rate to the survey, further investigation into the true burden of injury, as well as the risks that e-cigarettes pose, together with ways to reduce exposure, is needed.
Production of soluble pMHC-I molecules in mammalian cells using the molecular chaperone TAPBPR
Authors: O’Rourke Sara M , Morozov Giora I , Roberts Jacob T , Barb Adam W , Sgourakis Nikolaos G 
Published: July 2020

AbstractCurrent approaches for generating major histocompatibility complex (MHC) Class-I proteins with desired bound peptides (pMHC-I) for research, diagnostic and therapeutic applications are limited by the inherent instability of empty MHC-I molecules. Using the properties of the chaperone TAP-binding protein related (TAPBPR), we have developed a robust method to produce soluble, peptide-receptive MHC-I molecules in Chinese Hamster Ovary cells at high yield, completely bypassing the requirement for laborious refolding from inclusion bodies expressed in E.coli. Purified MHC-I/TAPBPR complexes can be prepared for multiple human allotypes, and exhibit complex glycan modifications at the conserved Asn 86 residue. As a proof of concept, we demonstrate both HLA allele-specific peptide binding and MHC-restricted antigen recognition by T cells for two relevant tumor-associated antigens. Our system provides a facile, high-throughput approach for generating pMHC-I antigens to probe and expand TCR specificities present in polyclonal T cell repertoires.
The Effect of the Credit Crunch on Output Price Dynamics: The Corporate Inventory and Liquidity Management Channel*
Authors: Kim Ryan 
Published: July 2020

Abstract
I study how a credit crunch affects output price dynamics. I build a unique micro-level data set that combines scanner-level prices and quantities with producer information, including the producer’s banking relationships, inventory, and cash holdings. I exploit the Lehman Brothers failure as a quasi-experiment and find that the firms facing a negative credit supply shock decrease their output prices approximately 15% more than their unaffected counterparts. I hypothesize that such firms reduce prices to liquidate inventory and generate additional cash flow from the product market. I find strong empirical support for this hypothesis: (i) the firms that face a negative bank shock temporarily decrease their prices and inventory and increase their market share and cash holdings relative to their counterparts, and (ii) this effect is stronger for the firms and sectors with a high initial inventory or small initial cash holdings.
Banks as Lenders of First Resort: Evidence from the COVID-19 Crisis
Authors: Li Lei , Strahan Philip E , Zhang Song 
Published: July 2020

Abstract
In March 2020, banks faced the largest increase in liquidity demands ever observed. Firms drew funds on a massive scale from preexisting credit lines in anticipation of cash flow and financial disruptions stemming from the advent of the COVID-19 crisis. The increase in liquidity demands was concentrated at the largest banks, who serve the largest firms. Precrisis financial condition did not constrain large banks’ liquidity supply. Coincident inflows of funds from both the Federal Reserve’s liquidity injection programs and depositors, along with strong preshock bank capital, explain why banks were able to accommodate these liquidity demands. (JEL G21, G28)
Received June 7, 2020; editorial decision June 23, 2020 by Editor Isil Erel.
Identifying the Real Effects of Zombie Lending
Authors: Schivardi Fabiano , Sette Enrico , Tabellini Guido 
Published: July 2020

Abstract
The policy response to COVID-19 includes the provision of credit guarantees to firms, a provision that may generate zombie lending. According to the recent literature, the relative performance of healthy firms deteriorates as the fraction of zombies increases. We argue that this literature faces a serious identification problem, because firm performance is often used to define zombies (sometimes implicitly). We show that, under general conditions for the distribution of firm performance, the correlation between healthy firm performance and zombies is a mechanical consequence of an increase in the fraction of zombies with no causal meaning. (JEL E44, G21)
Received June 2, 2020; editorial decision June 23, 2020 by Editor Uday Rajan.
Association of differentially expressed genes and autoantibody type in patients with systemic sclerosis
Authors: Inamo Jun 
Published: July 2020

Abstract

Objectives
The aims of this study were to investigate the relationship between the type of autoantibody and gene expression profile in skin lesions from patients with SSc, and to identify specific dysregulated pathways in SSc patients compared with healthy controls.

Methods
Sixty-one patients with SSc from the Genetics vs Environment in Scleroderma Outcome Study cohort and 36 healthy controls were included in this study. Differentially expressed genes were extracted and functional enrichment and pathway analysis were conducted.

Results
Compared with healthy controls, lists containing 2, 71, 10, 144 and 78 differentially expressed genes were created for patients without specific autoantibody, ACA, anti-U1 RNP antibody (RNP), anti-RNA polymerase III antibody (RNAP) and anti-topoisomerase I antibody (ATA), respectively. While part of the enriched pathways overlapped, distinct pathways were identified except in those patients lacking specific autoantibody. The distinct enriched pathways included ‘keratinocyte differentiation’ for ACA, ‘nuclear factor κB signalling’ and ‘cellular response to TGF-β stimulus’ for RNAP, ‘interferon α/β signalling’ for RNP, and ‘cellular response to stress’ for ATA. Cell type signature score analysis revealed that macrophages/monocytes, endothelial cells and fibroblasts were associated with ACA, RNAP, ATA and the severity of the SSc skin lesions.

Conclusion
Pathogenic pathways were identified according to the type of autoantibody by leveraging gene expression data of patients and controls from a multicentre cohort. The current study may promote the search for new therapeutic targets for SSc.
Epidemiologic study of patients with psoriatic arthritis in a real-world analysis: a cohort study of the French health insurance database
Authors: Pina Vegas Laura , Sbidian Emilie , Penso Laetitia , Claudepierre Pascal 
Published: July 2020

AbstractObjectivePsA is a chronic inflammatory arthritis with heterogeneous disease manifestations. Data on the prevalence of PsA in adults differ widely depending on the study and the country. This study aimed to estimate the prevalence and incidence of PsA in France, characterize comorbidities associated to PsA and identify prescribed treatments.MethodsThis nationwide cohort study involved the administrative healthcare database (Système National des Données de Santé) of the French health insurance scheme linked to the national hospital discharge database. All adults with PsA registered in the database and identified with a specific International Classification of Diseases, 10th revision code (M07) were included between 1 January 2015 and 31 December 2018.ResultsA total of 63 598 patients were identified as having PsA [55.9 years (s.d. 14.4), 45.6% males]. The prevalence of PsA was estimated at 0.1% and the incidence at 8.4 per 100 000 person-years in the general population. The most common comorbidities were hypertension, diabetes, chronic obstructive pulmonary disease and dyslipidaemia. The prevalence of treatment with conventional synthetic DMARDs (csDMARDs), biological or biosimilar DMARDs (b/bsDMARDs) and apremilast for PsA was 25.9% (16 453), 30.4% (19 325) and 3.5% (2231), respectively. Overall, 8966 (14.1%) patients were new users of csDMARDs, 8311 (13.1%) were new users of b/bsDMARDs and 1529 (7.4%) were new users of apremilast. The most common first-line csDMARD was methotrexate (70.9%) and the most frequent first-line b/bsDMARD was adalimumab (30.8%).ConclusionOur results lead to a better understanding of PsA. Results were similar to those from other published studies using other data sources, which highlights the reliability of insurance databases for studies.
Pleuroparenchymal fibroelastosis in rheumatic autoimmune diseases: a systematic literature review
Authors: Orlandi Martina , Landini Nicholas , Bruni Cosimo , Sambataro Gianluca , Nardi Cosimo , Bargagli Elena , Tomassetti Sara , Occhipinti Mariaelena , Bellando Randone Silvia , Guiducci Serena , Vancheri Carlo , Colagrande Stefano , Matucci-Cerinic Marco 
Published: July 2020

AbstractObjectivesPleuroparenchymal fibroelastosis (PPFE) is characterized by predominantly upper lobe pleural and subjacent parenchymal fibrosis; PPFE features were described in patients with rheumatic autoimmune diseases (RAID). A systematic literature review was performed to investigate the prevalence, prognosis and potential association of PPFE with previous immunosuppression in RAID.MethodsEMBASE, Web of Science and PubMed databases were questioned from inception to 1 September 2019. Articles published in English and addressing PPFE in patients with RAID were selected.ResultsTwenty out of 794 papers were selected with a total of 76 cases of RAID-PPFE patients (20 SSc, 9 RA, 6 IIM6 primary SS, 5 overlap syndromes, 3 ANCA-associated vasculitides, 2 granulomatosis with polyangiitis, 1 microscopic polyangiitis, 1 UCTD, 1 SLE, 1 GCA and 21 patients with non-specified RAID). Dyspnoea was the most frequently reported symptom (37/48 patients, 77%). Patients frequently presented with a restrictive pattern and decline in diffusing lung capacity for carbon monoxide. During the follow-up, 7/12 patients had progression at imaging, 22/39 presented a generic clinical worsening, 19/38 had a functional deterioration and 15/43 remained stable.ConclusionThe present systematic literature review confirms that PPFE features are present in RAID. Rheumatologists should be aware of this new radiological pattern that holds a bad prognosis.
ESTIMATION OF THERMAL POWER PLANT WORKERS EXPOSURE TO MAGNETIC FIELDS AND SIMULATION OF HAZARD ZONES
Authors: Bagheri Hosseinabadi Majid , Khanjani Narges , Ebrahimi Mohammad Hossein , Biganeh Jamal 
Published: July 2020

AbstractExtremely low-frequency magnetic fields (ELF-MFs) have raised some concerns due to their possible effects on workers’ health. In this study ELF-MFs were measured in different units of the thermal power plant based on gridding the indoor space. The exposure level was measured by spot measurement based on the IEEE Std C95.3.1 and then simulated in units with the highest magnetic field intensity by using ArcGIS software. The operators and balance of plant (BOP) technicians (12.64 ± 9.74 μT) and office workers (2.41 ± 1.22 μT) had the highest and lowest levels of both measured and estimated ELF-MFs exposure. The highest measured ELF-MFs were in the vicinity of the power transmission lines in the transformers’ building (48.2 μT). Our simulation showed the high and low exposure areas and ranked exposure well; but, the actual measurements of ELF-MFs exposure were in all cases higher than the estimated values, which means we still need to improve our estimations.
The impact of income support systems on healthcare quality and functional capacity in workers with low back pain: a realist review
Authors: Di Donato Michael , Iles Ross , Lane Tyler , Buchbinder Rachelle , Collie Alex 
Published: July 2020

Abstract
Low back pain (LBP) is a leading cause of work disability. While absent from work, workers with LBP may receive income support from a system such as workers' compensation or social security. This study examines how and in what contexts income support systems impact the healthcare quality for people with work disability and LBP and their functional capacity. We performed a realist review. Five initial theories about the relationship between income support systems and outcomes were developed, tested, and refined by acquiring and synthesising academic literature from purposive and iterative electronic database searching. This process was supplemented with gray literature searches for policy documents and semistructured interviews with experts in income support, health care, and LBP. Income support systems influence healthcare quality through funding restrictions, healthcare provider administrative burden, and allowing employers to select providers. They also influence worker functional capacity through the level of participation and financial incentives for employers, measures to prove the validity of the worker's LBP, and certain administrative procedures. These mechanisms are often exclusively context-dependent, and generate differing and unintended outcomes depending on features of the healthcare and income support system, as well as other contextual factors such as socioeconomic status and labour force composition. Research and policy design should consider how income support systems may indirectly influence workers with LBP through the workplace.