bluuebunny/crossref_metadata_2025_split huggingface dataset:
Parcellation of the neonatal cortex using Surface-based Melbourne Children’s Regional Infant Brain atlases (M-CRIB-S)
Authors: Adamson Chris L. , Alexander Bonnie , Ball Gareth , Beare Richard , Cheong Jeanie L. Y. , Spittle Alicia J. , Doyle Lex W. , Anderson Peter J. , Seal Marc L. , Thompson Deanne K. 
Published: March 2020

AbstractLongitudinal studies measuring changes in cortical morphology over time are best facilitated by parcellation schemes compatible across all life stages. The Melbourne Children’s Regional Infant Brain (M-CRIB) and M-CRIB 2.0 atlases provide voxel-based parcellations of the cerebral cortex compatible with the Desikan-Killiany (DK) and the Desikan-Killiany-Tourville (DKT) cortical labelling schemes. This study introduces surface-based versions of the M-CRIB and M-CRIB 2.0 atlases, termed M-CRIB-S(DK) and M-CRIB-S(DKT), with a pipeline for automated parcellation utilizing FreeSurfer and developing Human Connectome Project (dHCP) tools. Using T2-weighted magnetic resonance images of healthy neonates (n = 58), we created average spherical templates of cortical curvature and sulcal depth. Manually labelled regions in a subset (n = 10) were encoded into the spherical template space to construct M-CRIB-S(DK) and M-CRIB-S(DKT) atlases. Labelling accuracy was assessed using Dice overlap and boundary discrepancy measures with leave-one-out cross-validation. Cross-validated labelling accuracy was high for both atlases (average regional Dice = 0.79–0.83). Worst-case boundary discrepancy instances ranged from 9.96–10.22 mm, which appeared to be driven by variability in anatomy for some cases. The M-CRIB-S atlas data and automatic pipeline allow extraction of neonatal cortical surfaces labelled according to the DK or DKT parcellation schemes.
NFKB2 polymorphisms associate with the risk of developing rheumatoid arthritis and response to TNF inhibitors: Results from the REPAIR consortium
Authors: Manuel Sánchez-Maldonado Jose , Martínez-Bueno Manuel , Canhão Helena , ter Horst Rob , Muñoz-Peña Sonia , Moñiz-Díez Ana , Rodríguez-Ramos Ana , Escudero Alejandro , Sorensen Signe B. , Hetland Merete L. , Ferrer Miguel A. , Glintborg Bente , Filipescu Ileana , Pérez-Pampin Eva , Conesa-Zamora Pablo , García Antonio , den Broeder Alfons , De Vita Salvatore , Hove Jacobsen Svend Erik , Collantes Eduardo , Quartuccio Luca , Netea Mihai G. , Li Yang , Fonseca João E. , Jurado Manuel , López-Nevot Miguel Ángel , Coenen Marieke J. H. , Andersen Vibeke , Cáliz Rafael , Sainz Juan 
Published: March 2020

AbstractThis study sought to evaluate the association of 28 single nucleotide polymorphisms (SNPs) within NFKB and inflammasome pathway genes with the risk of rheumatoid arthritis (RA) and response to TNF inhibitors (TNFi). We conducted a case-control study in a European population of 1194 RA patients and 1328 healthy controls. The association of potentially interesting markers was validated with data from the DANBIO (695 RA patients and 978 healthy controls) and DREAM (882 RA patients) registries. The meta-analysis of our data with those from the DANBIO registry confirmed that anti-citrullinated protein antibodies (ACPA)-positive subjects carrying the NFKB2rs11574851T allele had a significantly increased risk of developing RA (PMeta_ACPA + = 0.0006) whereas no significant effect was found in ACPA-negative individuals (PMeta_ACPA− = 0.35). An ACPA-stratified haplotype analysis including both cohorts (n = 4210) confirmed that ACPA-positive subjects carrying the NFKB2TT haplotype had an increased risk of RA (OR = 1.39, P = 0.0042) whereas no effect was found in ACPA-negative subjects (OR = 1.04, P = 0.82). The meta-analysis of our data with those from the DANBIO and DREAM registries also revealed a suggestive association of the NFKB2rs1056890 SNP with larger changes in DAS28 (OR = 1.18, P = 0.007). Functional experiments showed that peripheral blood mononuclear cells from carriers of the NFKB2rs1005044C allele (in LD with the rs1056890, r2 = 1.00) showed increased production of IL10 after stimulation with LPS (P = 0.0026). These results provide first evidence of a role of the NFKB2 locus in modulating the risk of RA in an ACPA-dependent manner and suggest its implication in determining the response to TNFi. Additional studies are now warranted to further validate these findings.
Expression and distribution of CD151 as a partner of alpha6 integrin in male germ cells
Authors: Jankovicova J. , Frolikova M. , Palenikova V. , Valaskova E. , Cerny J. , Secova P. , Bartokova M. , Horovska L. , Manaskova-Postlerova P. , Antalikova J. , Komrskova K. 
Published: March 2020

AbstractThe physiological importance of CD151 tetraspanin is known from somatic cells and its outside-in signalling through integrins was described. In male germ cells, two tetraspanins, CD9 and CD81, are involved in sperm-egg membrane fusion, and similarly to integrins, they occupy characteristic regions. We report here on a newly discovered presence of CD151 in sperm, and present its expression and distribution during spermatogenesis and sperm transition during the acrosome reaction. We traced CD151 gene and protein expression in testicular cell subpopulations, with strong enrichment in spermatogonia and spermatids. The testicular and epididymal localization pattern is designated to the sperm head primary fusion site called the equatorial segment and when compared to the acrosome vesicle status, CD151 was located into the inner acrosomal membrane overlying the nucleus. Moreover, we show CD151 interaction with α6 integrin subunit, which forms a dimer with β4 as a part of cis-protein interactions within sperm prior to gamete fusion. We used mammalian species with distinct sperm morphology and sperm maturation such as mouse and bull and compared the results with human. In conclusion, the delivered findings characterise CD151 as a novel sperm tetraspanin network member and provide knowledge on its physiology in male germ cells.
Poly(Ethylene Oxide)-based Electrolyte for Solid-State-Lithium-Batteries with High Voltage Positive Electrodes: Evaluating the Role of Electrolyte Oxidation in Rapid Cell Failure
Authors: Homann Gerrit , Stolz Lukas , Nair Jijeesh , Laskovic Isidora Cekic , Winter Martin , Kasnatscheew Johannes 
Published: March 2020

AbstractPolyethylene oxide (PEO)-based solid polymer electrolytes (SPEs) typically reveal a sudden failure in Li metal cells particularly with high energy density/voltage positive electrodes, e.g. LiNi0.6Mn0.2Co0.2O2 (NMC622), which is visible in an arbitrary, time – and voltage independent, “voltage noise” during charge. A relation with SPE oxidation was evaluated, for validity reasons on different active materials in potentiodynamic and galvanostatic experiments. The results indicate an exponential current increase and a potential plateau at 4.6 V vs. Li|Li+, respectively, demonstrating that the main oxidation onset of the SPE is above the used working potential of NMC622 being < 4.3 V vs. Li|Li+. Obviously, the SPE│NMC622 interface is unlikely to be the primary source of the observed sudden failure indicated by the “voltage noise”. Instead, our experiments indicate that the Li | SPE interface, and in particular, Li dendrite formation and penetration through the SPE membrane is the main source. This could be simply proven by increasing the SPE membrane thickness or by exchanging the Li metal negative electrode by graphite, which both revealed “voltage noise”-free operation. The effect of membrane thickness is also valid with LiFePO4 electrodes. In summary, it is the cell set-up (PEO thickness, negative electrode), which is crucial for the voltage-noise associated failure, and counterintuitively not a high potential of the positive electrode.
Mitochondria as target to inhibit proliferation and induce apoptosis of cancer cells: the effects of doxycycline and gemcitabine
Authors: Dijk Sas N. , Protasoni Margherita , Elpidorou Marilena , Kroon Albert M. , Taanman Jan-Willem 
Published: March 2020

AbstractDoxycycline has anti-tumour effects in a range of tumour systems. The aims of this study were to define the role mitochondria play in this process and examine the potential of doxycycline in combination with gemcitabine. We studied the adenocarcinoma cell line A549, its mitochondrial DNA-less derivative A549 ρ° and cultured fibroblasts. Treatment with doxycycline for 5 days resulted in a decrease of mitochondrial-encoded proteins, respiration and membrane potential, and an increase of reactive oxygen species in A549 cells and fibroblasts, but fibroblasts were less affected. Doxycycline slowed proliferation of A549 cells by 35%. Cellular ATP levels did not change. Doxycycline alone had no effect on apoptosis; however, in combination with gemcitabine given during the last 2 days of treatment, doxycycline increased caspase 9 and 3/7 activities, resulting in a further decrease of surviving A549 cells by 59% and of fibroblasts by 24% compared to gemcitabine treatment alone. A549 ρ° cells were not affected by doxycycline. Key effects of doxycycline observed in A549 cells, such as the decrease of mitochondrial-encoded proteins and surviving cells were also seen in the cancer cell lines COLO357 and HT29. Our results suggest that doxycycline suppresses cancer cell proliferation and primes cells for apoptosis by gemcitabine.
Transplantation of a 3D-printed tracheal graft combined with iPS cell-derived MSCs and chondrocytes
Authors: Kim In Gul , Park Su A. , Lee Shin-Hyae , Choi Ji Suk , Cho Hana , Lee Sang Jin , Kwon Yoo-Wook , Kwon Seong Keun 
Published: March 2020

AbstractFor successful tracheal reconstruction, tissue-engineered artificial trachea should meet several requirements, such as biocompatible constructs comparable to natural trachea, coverage with ciliated respiratory mucosa, and adequate cartilage remodeling to support a cylindrical structure. Here, we designed an artificial trachea with mechanical properties similar to the native trachea that can enhance the regeneration of tracheal mucosa and cartilage through the optimal combination of a two-layered tubular scaffold and human induced pluripotent stem cell (iPSC)-derived cells. The framework of the artificial trachea was fabricated with electrospun polycaprolactone (PCL) nanofibers (inner) and 3D-printed PCL microfibers (outer). Also, human bronchial epithelial cells (hBECs), iPSC-derived mesenchymal stem cells (iPSC-MSCs), and iPSC-derived chondrocytes (iPSC-Chds) were used to maximize the regeneration of tracheal mucosa and cartilagein vivo. After 2 days of cultivation using a bioreactor system, tissue-engineered artificial tracheas were transplanted into a segmental trachea defect (1.5-cm length) rabbit model. Endoscopy did not reveal granulation ingrowth into tracheal lumen. Alcian blue staining clearly showed the formation of ciliated columnar epithelium in iPSC-MSC groups. In addition, micro-CT analysis showed that iPSC-Chd groups were effective in forming neocartilage at defect sites. Therefore, this study describes a promising approach for long-term functional reconstruction of a segmental tracheal defect.
Stigmasterol alleviates cerebral ischemia/reperfusion injury by attenuating inflammation and improving antioxidant defenses in rats
Authors: Liang Qilong , Yang Jun , He Jiaji , Chen Xiaoling , Zhang Hong , Jia Maolin , Liu Kai , Jia Chuangchuang , Pan Yanhong , Wei Jinwang 
Published: March 2020

Abstract
Background/aims: The paper aimed to investigate the effects of Stigmasterol on inflammatory factors, antioxidant capacity, and apoptotic signaling pathways in brain tissue of rats with cerebral ischemia/reperfusion (I/R) injury. Methods: The neurological deficits of the rats were analyzed and HE staining was performed. The cerebral infarct volume was calculated by means of TTC staining, and neuronal apoptosis was detected by TUNEL staining. At the same time, the contents of glutathione peroxidase, glutathione, superoxide dismutase (SOD), nitric oxide, and malondialdehyde in brain tissue were measured. The expression of the relevant protein was detected by means of Western blotting. Results: The results showed that the neurological deficit score and infarct area of the I/R rats in the soy sterol treatment group were significantly lower than those in the I/R group. Moreover, the levels of carbon monoxide and malondialdehyde in the soysterol group were significantly lower than those in the I/R group, and the expressions of cyclooxygenase-2 (Cox-2) and NF-κB (p65) in the soysterol group were also significantly lower than those in the I/R group. The expression of Nrf2 (nucleus) and heme oxygenase-1 (HO-1) increased significantly, and the activities of antioxidant enzymes and SOD were increased. In addition, the stigmasterol treatment can inhibit apoptosis, down-regulate Bax and cleaved caspase-3 expression, and up-regulate Bcl-Xl expression. Conclusion: Stigmasterol protects the brain from brain I/R damage by reducing oxidative stress and inflammation.
Dietary vitamin A intake and the risk of ovarian cancer: a meta-analysis
Authors: Wang Qiaoqiao , He Chaying 
Published: March 2020

Abstract
BACKGROUND: Previous studies have demonstrated some associations between dietary vitamin A intake and ovarian cancer risk with an inconsistent relationship. We therefore performed the present study to further explore the association between them.
METHODS: Databases of PubMed, Embase, and Web of Science were retrieved up to September 1, 2019. Summarized relative risk (RR) with corresponding 95% confidence intervals (CI) were calculated. Stata 14.0 software was used for data analysis.
RESULTS: Fifteen articles involving 4882 cases and 443,179 participants were included in this meta-analysis. A positive association between dietary vitamin A intake and ovarian cancer risk was found (RR = 0.816, 95%CI = 0.723–0.920, I2 = 48.4%, Pfor heterogeneity = 0.019). Significant association was also found in case–control studies (RR = 0.769, 95%CI = 0.655–0.902), but not in cohort studies. When we performed the analysis between ovarian cancer risk and geographic locations, we found an inverse association in North American populations (RR = 0.825, 95%CI = 0.720–0.946), instead of other populations.
CONCLUSIONS: In summary, findings from the present study suggested that higher dietary intake of vitamin A may contribute to the lower development of ovarian cancer, especially among North Americans.
La muqueuse péri-implantaire : nécessité esthétique ou fonctionnelle ? Intérêt de l’augmentation de tissus mous peri-implantaires
Authors: Aroca S. 
Published: March 2020

L’anneau de tissu mou formé autour du col de l’implant est appelé muqueuse péri-implantaire. Un des rôles de cette attache tissulaire est de créer un joint biologique protecteur autour de l’implant afin de prévenir des maladies inflammatoires (mucosite, peri-implantite) qui pourraient compromettre lostéointegration.
Ce rôle protecteur est une évidence clinique malgré les controverses existantes dans la littérature en relation avec la nécessité de la présence ou absence des tissus kératinisés autour des implants. Mais quelle épaisseur tissulaire est capable d’assurer une stabilité du joint biologique péri-implantaire ? Le tissu conjonctif joue-t-il un rôle prépondérant ? La stabilité du joint biologique, est-elle liée à l’épaisseur des tissus, à la stabilité des tissus osseux ou bien aux deux ?
Par ailleurs, labsence de muqueuse kératinisée, un phenotype fin, aussi une insuffisance de volume, tant des situations cliniques qui risquent de favoriser linstabilité des tissus péri-implantaires.
Les conséquences cliniques (outre le risque de maladie inflammatoire) sont multiples :

1
Mobilité et ou décollement de la muqueuse péri-implantaire
2
Récession de la muqueuse péri-implantaire
3Restauration implanto-portée inesthétique par rapport aux dents adjacentes

Cet exposé a pour but de répondre à toutes ces questions et aussi de détailler les solutions qui sont nécessaires pour assurer ou rétablir une stabilité de la muqueuse péri-implantaire.
Translationale Highlights Mamma- und Ovarialkarzinom 2019 – Immuntherapien, DNA-Reparatur, PI3K-Inhibition und CDK4/6-Therapien
Authors: Hartkopf Andreas D. , Müller Volkmar , Wöckel Achim , Lux Michael P. , Janni Wolfgang , Ettl Johannes , Belleville Erik , Schütz Florian , Fasching Peter A. , Kolberg Hans-Christian , Welslau Manfred , Overkamp Friedrich , Taran Florin-Andrei , Brucker Sara Y. , Wallwiener Markus , Tesch Hans , Fehm Tanja N. , Schneeweiss Andreas , Lüftner Diana 
Published: March 2020

ZusammenfassungIm Rahmen von derzeit laufenden Studien werden in naher Zukunft wichtige translationale Fragestellungen von klinischer Relevanz geklärt werden. Zum einen muss die Rolle der PD‑L1-Expression, nachdem sie für die Indikationsstellung für Atezolizumab in der 1. Therapielinie bei Patientinnen mit einem metastasierten triplenegativen Mammakarzinom (TNBC) von Relevanz ist, weiter geklärt werden. In einer neoadjuvanten Studie beim TNBC mit Pembrolizumab konnte kein Zusammenhang zwischen Wirksamkeit und PD‑L1-Expression gefunden werden. Sowohl mit als auch ohne Expression war die pCR höher, wenn Pembrolizumab zur Chemotherapie hinzugefügt wurde. Des Weiteren wird für die bei Keimbahnmutation von BRCA1/2 zugelassenen PARP-Inhibitoren nach weiteren Patientengruppen gesucht, in denen eine Wirksamkeit besteht. Dieses sind z. B. Patientinnen mit Mutationen in anderen Genen, die an der homologen Rekombination beteiligt sind, oder Patientinnen mit Tumoren, die bei globalen Tests zu homologen Rekombinationsdefizienzen (HRDTests) eine Auffälligkeit zeigen. Auch die Frage, ob und mit welchen Chemotherapie-Kombinationspartnern eine PARP-Inhibition zusammen gegeben werden kann, wird derzeit sowohl beim Mamma- als auch beim Ovarialkarzinom untersucht. Während sich die Daten zum verbesserten Gesamtüberleben bei den CDK4/6-Inhibitoren konsolidieren, wächst auch das Wissen um molekulare Veränderungen unter der Therapie und bei der Progression unter der Therapie. Sowohl die Anhäufung von PI3K-Mutationen als auch PTEN-Veränderungen könnten bei der Planung von Folgetherapien eine Rolle spielen. Diese Übersichtsarbeit fasst diese aktuellen Entwicklungen beim Mammakarzinom und teilweise auch beim Ovarialkarzinom zusammen.
Association between Vitamin D and Knee Osteoarthritis: A PRISMA-Compliant Meta-analysis
Authors: Yu Yinghao , Liu Dongcheng , Feng Dehong , Zhao Jijun 
Published: March 2020

Abstract
Background Previous investigations showed inconsistent results for associations between vitamin D and knee osteoarthritis (OA). The present study aimed to make a systematic review and meta-analysis for the association between vitamin D and knee OA.
Methods We systematically searched for articles published in the databases PubMed, Web of Science, EMBASE, Medline, and Google Scholar through July 2019. All statistical analyses were made using STATA 12.0 software. The Q test and I2 were applied to examine heterogeneities between studies.
Results The study indicated that there were no significant associations between serum levels of 25-hydroxy (OH) vitamin D and prevalence, incidence or progression of knee radiographic OA (ROA), and joint space narrowing (JSN). However, a subgroup study showed significant associations of low vitamin D levels with the progression of knee OA. Additionally, the present study showed no significant effect of vitamin D supplementation on knee OA.
Conclusions The results do not support that serum levels of 25(OH) vitamin D are associated with the risks of knee OA, except perhaps with progression of knee OA. In addition, vitamin D supplementation may not have a clinically significant effect on pain control or structure (tibial cartilage volume and JSW) progression in patients with knee OA. Longer-term clinical trials are essential to explore the effect of vitamin D supplementation on knee OA.
Prevalence of Dental Caries in 5- to 12-Year-Old School Children of Patiala City, Punjab
Authors: Kaur Surinder , Kaur Avninder , Singh Reetu , Avasthi Avijit , Fatima Alvi 
Published: March 2020

Abstract
Introduction The present study was conducted with the aim to evaluate prevalence of dental caries in 5- to 12-year-old children in the city of Patiala, Punjab.
Materials and Methods This cross sectional study comprised 1,600 school children belonging to upper middle and lower middle class groups. The children were examined in their schools itself sitting on an ordinary chair.
Results Caries prevalence reported was 40%. Higher decayed, missing, and filled teeth were found in children eating sweet foods.
Conclusion Results reveal a high percentage of children with dental caries which shows a need for oral health awareness in these children. A low percentage of children was brushing twice a day.
Families' Experiences of Life in the Year after a Child's Critical Illness: Navigating the Road to a “New Normal”
Authors: Atkins Ellie K. , John Mary , Colville Gillian 
Published: March 2020

AbstractSemistructured interviews were conducted with patients and family members (n = 18) postdischarge to examine how they managed their recovery in the year following a child's intensive care unit admission. Data were analyzed using the grounded theory. Participants described an ongoing need to develop and adapt their narratives about admission and recovery. Other key themes were as follows: just getting through, recognizing they had changed, striving for normality, and finding positive aspects related to their experiences. It took longer than they expected for families to build a coherent narrative of events postdischarge and to adjust to the “new normal.” Implications for health professionals are discussed.
High-Dose Vasopressor Therapy for Pediatric Septic Shock: When Is Too Much?
Authors: da Silva Paulo Sérgio Lucas , Fonseca Marcelo Cunio Machado 
Published: March 2020

AbstractIt is unknown if the requirement for high dose of vasopressor (HDV) represents a poor outcome in pediatric septic shock. This is a retrospective observational analysis with data obtained from a single center. We evaluated the association between the use of HDV and survival in these patients. A total of 62 children (38 survivors and 24 nonsurvivors) were assessed. The dose of vasopressor (hazard ratio 2.06) and oliguria (hazard ratio 3.17) was independently associated with mortality. The peak of vasopressor was the best prognostic predictor. A cutoff of 1.3 μg/kg/min was associated with mortality with a sensitivity of 75% and specificity of 89%. Vasopressor administration higher than 1.3 μg/kg/min was associated with increased mortality in children with septic shock.
Complete Labyrinthine Aplasia: A Unique Sign for Targeted Genetic Testing in Hearing Loss
Authors: Lallar Meenakshi , Arora Veronica , Saxena Renu , Puri Ratna Dua , Verma Ishwar Chander 
Published: March 2020

AbstractComplete labyrinthine aplasia (CLA) is a rare inner ear anomaly. The only identified genetic cause of CLA with severe sensorineural hearing loss is labyrinthine aplasia, microtia, and microdontia (LAMM) syndrome. Here we reported a child who presented with syndromic hearing loss and was diagnosed with LAMM syndrome. Genetic evaluation provided the family with confirmation of the diagnosis, provision of the prognosis, genetic counselling, and prenatal diagnosis. This report highlighted that CLA should be recognized as a unique sign to diagnose LAMM syndrome, to analyze FGF3 gene mutation, and also demonstrated the utility of genetic testing in patients with suspected LAMM syndrome to provide exact diagnosis and further management.