bluuebunny/crossref_metadata_2025_split huggingface dataset:
Various combinations of Carbapenemase production in community-acquired clinical isolates in Nepal
Authors: Sah Ranjit , Rodriguez-Morales Alfonso J. , Pokhrel Nayanum , Donovan Suzanne , Shrestha Gentle Sunder , Fazal Farhan , Singla Sonam , Sencan Mehtap , Rabaan Ali A. , Kumari Manisha , Sah Sanjit , Khadka Shusila , Adhikari Mahesh , Erdem Hakan 
Published: February 2020

Abstract
Background Carbapenems are at the forefront of managing infections caused by multidrug-resistant organisms (MDROs). These antibiotics are destroyed by carbapenemase enzymes, produced by the infecting bacteria. Klebsiella pneumoniae Carbapenemase (KPC), New Delhi Metallo - β lactamase (NDM), Verona integron-encoded Metallo β lactamase (VIM), OXA-48, and IMP are the commonest carbapenemases reported. Since these resistance phenotypes are related to hospitals, their place in the community is actually unclear and unexpected. Our case series is, therefore, crucial to reveal the significant production of multiple carbapenemases in these alarmingly resistant community-acquired isolates recovered from three patients. Case presentation We confirmed carbapenemase production by using the modified Hodge test (MHT) and Xpert Carba R. NDM, KPC and OXA-48 carbapenemase production, VIM, NDM, KPC, and OXA carbapenemase production, and IMP, VIM, NDM, and OXA carbapenemase production were demonstrated in Case 1, Case 2, and Case 3, respectively. Escherichia coli was isolated from blood and urine samples of case 1. The isolate was susceptible to colistin, tigecycline, and fosfomycin. Due to the development of acute kidney injury, treatment with colistin combined with tigecycline was stopped and Inj. Fosfomycin was started intravenously. After completion of antimicrobial therapy, the patient successfully recovered. Blood and sputum cultures of Case 2 with community-acquired pneumonia, yielded multidrug-resistant Klebsiella pneumonia . The strain was carbapenemase producer and susceptible to tigecycline and colistin solely. Treatment with intravenous colistin and tigecycline resulted in a good clinical outcome. E. coli was recovered from both the ascitic fluid and the blood of Case 3.The pathogen was susceptible to both colistin and tigecycline. After the modification of treatment modality from intravenous imipenem to tigecycline, the patient began to improve on the third day of therapy. Conclusions Our results are noteworthy in emphasizing the growing resistance profiles of gram-negative bacteria in the community and show an epidemiological link between healthcare settings and the community. These bacteria are easily transmissible by mobile genetic elements (MGEs), compelling us to rely on last-resort drugs like colistin, tigecycline, or fosfomycin for better therapeutic management. Accordingly, we used these drugs in combination, and all three patients were cured.
Cost-effectiveness of Comprehensive screening of general population for hypertension: Can it save money and Life? Systematic review of Pharmacoeconomic Studies
Authors: Davari Majid , Sorato Mende Mensa , Nikfar Shekoufeh 
Published: February 2020

Abstract
Background: Hypertension is one of major modifiable risk factors contributing for development of ischemic heart disease, diabetes, kidney disease, cerebrovascular disease and peripheral arterial disease. Silent nature of the disease, delayed presentation of patients to health system after development of significant cardiovascular events and poor access to comprehensive health care are major challenge of hypertension control. Early screening, detection and treatment of hypertension is effective for control of the disease progression. However, there is no robust evidence on whether screening general population for hypertension is cost-effective or not. Therefore, this review was conducted t o generate evidence on cost effectiveness of population-based hypertension screening for asymptomatic individuals as early detection strategy for the primary prevention of cardiovascular diseases. Methods: PubMed/Medline , Scopus, Web of sciences and Google Scholar were searched from January 2000 to 11 December 2019. Two investigators independently selected and reviewed fair and good-quality pharmacoeconomic studies for the cost-effectiveness of asymptomatic screening for hypertension in the community. Quality of selected literatures are evaluated by authors based on comprehensive tool developed for critical appraisal of pharmacoeconomic studies. Results: Eleven included Pharmacoeconomic studies reported favorable results for screening asymptomatic adults for hypertension. Most of studies agreed on cost-effectiveness of screening adults aged 40 years and older. Screening of general adult population for hypertension is not-cost effective. Conclusion: Screening population 40 years and older with or without additional risk factors is cost-effective in reducing hypertension and associated cardiovascular disease morbidity and mortality in developed and developing countries.
Label-free Quantitative Proteomics for Investigating the Therapeutic Mechanism of Sijunzi Decoction on Spleen Deficiency Syndrome
Authors: Yan Qinghua , Ma Shijiang , Chen Hongli , Zhang Qiqing 
Published: February 2020

Abstract
Background: Sijunzi decoction is a traditional Chinese medicine prescription which is used to treat spleen deficiency syndrome. But the therapeutic mechanism of Sijunzi decoction on spleen deficiency syndrome is unclear.Methods: A rat model of reserpine-induced spleen deficiency syndrome was established. The therapeutic effects of Sijunzi decoction were assessed by histopathological examination and enzyme activity analysis. A label-free proteomics method was used to assess the changes in the endogenous protein. The differentially expressed proteins (DEPs) were analyzed using bioinformatics methods. Western blot was used to validate the proteomic data.Results: Sijunzi decoction could significantly restore damaged liver tissue and enzymatic activities. DEPs of Sijunzi decoction-intervened spleen deficiency syndrome mainly regulated various metabolic processes involved in cellular responses to enzyme activity for catalysing, transforming and transferring. The four pathways (bile secretion, pyruvate metabolism, cysteine and methionine metabolism, and fatty acid metabolism) might be closely related with the intervention mechanism of Sijunzi decoction. Protein H2afx, interferon stimulated gene 15 protein, and signal transducers and activators of transcription1 proteins were predicted to play the important roles in Sijunzi decoction action networks.Conclusions: Sijunzi decoction could exert intervention action by restoring DEPs involved in potential target pathways. Current results wish to provide novel perspectives for revealing the potential protein targets of Sijunzi decoction on spleen deficiency syndrome and facilitating its clinical usage.
Protein arginine methyltransferase-5 Selective Inhibitor Enhances Therapeutic Effects of Cisplatin on Lung Cancer Cells
Authors: Bajbouj Khuloud , Ramakrishnan Rakhee K. , Saber-Ayad Maha , Omar Hany A. , Sharif-Askari Narjes Saheb , Shafarinhttps://www.editorialmanager.com/bcan/SubM Jasmin , Elmoselhi Adel , Ihmaid Ahmed , Ali Suhib Al Haj , Alalool Abdulla , Abdullah Reem , Hamid Qutayba 
Published: February 2020

Abstract
Background Lung cancer is the most common cancer globally. Protein arginine methyltransferase 5 (PRMT5) is identified to be involved in gene transcriptional regulation and cell division. PRMT5 is highly expressed in lung adenocarcinoma, hepatocellular carcinoma, and melanoma, raising evidence that PRMT5 might be involved in tumorigenesis. The aim of this study is to examine potential selective anti-neoplastic activity of PRMT5 inhibitor, Arginine methyltransferase inhibitor 1 (AMI-1) and cisplatin on lung adenocarcinoma. Methods Effect of AMI-1 on PRMT5 activity inhibition in lung adenocarcinoma cell line, A549, in response to standard chemotherapeutic agent, cisplatin, was assessed. Bioinformatic analyses were carried out to identify the prognostic value of PRMT5 and its major functional pathways in lung adenocarcinoma. Cell viability, PMRT5 protein levels, extent of cell migration, survival of cancer cells, and cell cycle progression and apoptosis were examined. Drug combination was also evaluated in human bronchial epithelial cells (HBEpC). Results Bioinformatics identified apoptosis, DNA damage, and cell cycle progression as the main PRMT5 associated functional pathways, and survival analysis linked the increased PRMT5 gene expression to worse overall survival. Combination treatment with 10 µM AMI-1 and of Cisplatin significantly reduced viable cell percentages. Cell cycle arrest in A549 cells was evident after AMI-1, which was reinforced after combination treatment. Apoptosis was observed after treatment with both drugs. Western blot analysis showed reduction in demethylation histone 4, a PRMT5- downstream target, after treatment with AMI-1 alone or in combination with cisplatin. While combination approach tackled lung cancer cell survival, it exhibited cytoprotective abilities on normal epithelial cells. Finally, treatment with both drugs led to a decreased cell migration rate. Conclusions This study highlights evidence of novel selective antitumor additive activity of AMI-1 in combination with cisplatin in lung adenocarcinoma cells. Survival of normal bronchial epithelial cells was not affected by using AMI-1.
The Valuable Impacts of Halophytic Genus Suaeda; Nutritional, Chemical, and Biological Values
Authors: Mohammed Hamdoon A. 
Published: February 2020

Background:
Suaeda is a halophytic genus belonging to the Amaranthaceae family and
can survive in the high salted marsh areas of the world. Suaeda plants can biosynthesize natural
substances with powerful antioxidant activity and are considered as a renewable source of energy,
food, and edible oil for a larger number of populations living in the harsh environment with high
salinity and drought conditions. These plants also meet folk and alternative medicines' needs.

Methods:
The review encompasses available scientific literature related to folk medicinal uses of
Suaeda plants, their nutritional values, and chemical constituents. In addition, the biological trials
applied for the Suaeda plants are also part of the review. The review covers the researches from
major science literature search engines and other sites representing scientific literature, i.e., Scifinder,
Google Scholar, PubMed, ScienceDirect, Scopus, and Google. The searches were programmed
on the advance options available in the search engines and are latest up to November
2019. The searches were exhaustive and rechecked for accuracy.

Conclusion:
The study summarizes the uses of Suaeda plants as a remedy for various ailments due
to their contents from the polyphenols and flavonoids. The comparatively large amounts of fixed
oils, minerals, and vitamins in Suaeda plants have also made them potential renewable sources for
foods.
Physicochemical Assessment of Branded and Generic Linezolid Injection from Different Pharmaceutical Manufacturers
Authors: Zhang Su-hua , Wei Shan-shan , Zhu Zhen-yu , Chen Ying , Cai Hua-lin , Zhang Bi-kui 
Published: February 2020

Background:
Up to date, generic linezolid injections produced by Chinese manufacturers
were not widely used in clinics in China. Quality evaluation of linezolid injections produced in China
is a prerequisite, which has rarely been performed.

Objective:
This study aimed to evaluate the quality of branded and generic injections from different
manufacturers and to provide a basis for quality control.

Methods:
In this study, the content of linezolid, related impurities and enantiomer of linezolid were
determined by high-performance liquid chromatography. The content of glucose was determined by the
iodine method. The insoluble particles and visible and sub-visible particles were determined by light
blockage and lamp test, respectively. Osmotic pressure was determined by the freezing point depression
method. The standard solution control method was used to check the color of the injection. Linezolid
injections from different manufacturers were evaluated uniformly.

Results:
No significant difference was found in the content of linezolid, glucose, related impurities,
visible particles, insoluble particles, pH value, and solution color between branded and generic drugs
from different manufacturers in China.

Conclusion:
The quality of samples from different manufacturers is consistent. Although the physicochemical
similarity does not guarantee the bioequivalence of studied branded and generic linezolid injections,
the results provide references for further bioequivalence study. Generic injections offer more
affordable treatment options for patients with infections than expensive branded drugs.
Comparisons of MicroRNA Set Enrichment Analysis Tools on Cancer De-regulated miRNAs from TCGA Expression Datasets
Authors: Li Jianwei , Liu Leibo , Cui Qinghua , Zhou Yuan 
Published: February 2020

Background:
De-regulation of microRNAs (miRNAs) is closely related to many complex
diseases, including cancers. In The Cancer Genome Atlas (TCGA), hundreds of differentially
expressed miRNAs are stored for each type of cancer, which are hard to be intuitively interpreted.
To date, several miRNA set enrichment tools have been tailored to predict the potential disease
associations and functions of de-regulated miRNAs, including the miRNA Enrichment Analysis and
Annotation tool (miEAA) and Tool for Annotations of human MiRNAs (TAM1.0 &TAM 2.0).
However, independent benchmarking of these tools is warranted to assess their effectiveness and
robustness, and the relationship between enrichment analysis results and the prognosis significance
of cancers.

Methods:
Based on differentially expressed miRNAs from expression profiles in TCGA, we
performed a series of tests and a comprehensive comparison of the enrichment analysis results of
miEAA, TAM 1.0 and TAM 2.0. The work focused on the performance of the three tools, disease
similarity based on miRNA-disease associations from the enrichment analysis results, the
relationship between the overrepresented miRNAs from enrichment analysis results and the
prognosis significance of cancers.

Results:
The main results show that TAM 2.0 is more likely to identify the regulatory disease’s
functions of de-regulated miRNA; it is feasible to calculate disease similarity based on enrichment
analysis results of TAM 2.0; and there is weak positive correlation between the occurrence
frequency of miRNAs in the TAM 2.0 enrichment analysis results and the prognosis significance of
the cancer miRNAs.

Conclusion:
Our comparison results not only provide a reference for biomedical researchers to
choose appropriate miRNA set enrichment analysis tools to achieve their purpose but also
demonstrate that the degree of overrepresentation of miRNAs could be a supplementary indicator of
the disease similarity and the prognostic effect of cancer miRNAs.
Deployment of Cost-Efficient Cloud Integrated WOBAN: A Cluster- Based Approach
Authors: Bhatt Uma Rathore , Chouhan Nitin , Marmat Antim Bala , Upadhyay Raksha 
Published: February 2020

Aim:
To deploy Cloud Components in a cloud integrated WOBAN in a cost-efficient
manner.

Background:
Wireless-Optical Broadband Access Network (WOBAN) is a network which provides
higher bandwidth and better stability at lower cost to the users. Integration of cloud services over traditional
WOBAN called as CIW (cloud integrated WOBAN) increases existing load carrying capacity
and available bandwidth. In CIW, Cloud Components (CCs) are integrated as storage components,
servers etc. within WMN of WOBAN. These cloud components may follow distributed or centralized
architectures. Availability of these low-cost and easy-to-deploy CCs make CIW technologically
feasible and practically deployable architecture.

Objective:
The proposed algorithm eliminates redundant ONUs and optimizes number of CCs while
ensuring network connectivity and cloud services to all users.

Methods:
Extensive simulation is carried out to implement proposed work. A simulation model and
code is developed in MATLAB to get the results for existing and proposed algorithms for varying
number of variables such as number of ONUs, wireless routers, grid sizes etc.
Result: We compare the performance of proposed algorithm with existing algorithm for varying grid
sizes as well as number of wireless routers. Simulation results show the worthiness of proposed
work.

Conclusion:
Optimization of cloud components in CIW is one of the important issues as it affects
overall network deployment cost. Considering this issue, we proposed a cluster based approach to
optimize number of network components such as CCs and ONUs in the network. Simulation results
show that cluster based approach is efficient as compared to existing approach for optimizing CCs.
Hence proposed solution offers a suitable candidature for deploying a cost efficient CIW.
Carbon Nanotubes for Amplification of Electrochemical Signal in Drug and Food Analysis; A Mini Review
Authors: Karimi Fatemeh , Zakariae Nilofar , Esmaeili Roghayeh , Alizadeh Marzieh , Tamadon Ali-Mohammad 
Published: February 2020

Background:Electrochemical sensors are widely used for the determination of drugs and food compounds. In recent years, the amplification of electrochemical signals with nanomaterials, especially Carbon Nanotubes (CNTs) has created a major revolution in electrochemistry.Objective:The present mini-review paper focused on studying the role of CNTs as conductive mediators for the fabrication of highly sensitive electrochemical sensors. CNTs, with high conductivity and good ability for modification with other materials, are interesting candidates for improving the sensitivity of electrochemical sensors. CNTs or their derivatives are suggested for different applications in electrochemistry and especially analytical biosensors. This review is aimed to discuss the conductivity feature of CNTs in electrochemical sensors.
Pembelajaran batik tetes lilin sebagai alternatif teknik membatik sederhana pada mahasiswa PAUD
Authors: Prayitno Prayitno 
Published: February 2020

Tujuan penelitian ini adalah untuk mendeskripsikan atau menggambarkan bagaimana pembelajaran membuat batik dengan teknik tetes lilin terhadap mahasiswa Pendidikan Anak Usia Dini (PAUD). Metode penelitian ini menggunakan metode deskriptif. Subjek penelitian ini adalah mahasiswa PAUD salah satu Perguruan Tinggi Negeri di Yogyakarta, sebanyak 36 mahasiswa. Batik tetes lilin adalah teknik membatik pada kain dengan menggunakan media tetesan lilin sebagai perintang warna dan media untuk menghasilkan motif (pengganti canting). Dari hasil penelitian yang didapat menunjukkan bahwa sebagian besar mahasiwa PAUD ini dapat membuat batik tetes lilin. Hal itu dapat dilihat dari hasil karya yang dibuat oleh mahasiswa PAUD tersebut. Hasil karya mahasiswa yang mendapat kriteria Baik 53%, kriteria Cukup 33%, dan Kurang 14%. Pembelajaran batik tetes lilin ini dapat dijadikan sebagai alternatif pembelajaran membatik sederhana. Setelah melakukan pembelajaran batik dengan teknik tetes lilin ini, diharapkan Mahasiswa PAUD ketika sudah mengajar dapat mengaplikasikannya terhadap anak didiknya nanti di sekolah. Pembelajaran batik sederhana ini sebagai referensi Mahasiswa PAUD, dapat dikembangkan lagi dan dapat disesuaikan dengan kondisi anak didiknya nanti. AbstractThe purpose of this research was to descript or to describe learning to make a batik with drops candle technic to Early Childhood Education college students. The method of this research used described method. The subject of this research is Early Childhood Education student colleges one of the Public Universities in Yogyakarta, as many 36 student colleges. Batik Tetes Lilin is technique to make batik on fabric with use candle drops as covering the color and media to produce the motives (replacement of canting). Results of research obtained shows that most of the Early Childhood Education student colleges can be make a Batik Tetes Lilin. The creation student colleges have got good criteria is 53%, enough criteria is 33%, and less criteria is 14%. Learning of Batik Tetes Lilin can be used as alternative learning to make simple batik. After did learning of batik with drops candle technic, expected the Early Childhood Education college student’s can apply to students at school. Learning of simple batik as an reference Early Childhood Education college students, can be develop again and can adjust with condition of students.
Alat permainan edukatif berbasis lingkungan untuk pembelajaran saintifik tema lingkungan bagi guru paud korban gempa
Authors: Astini Baik Nilawati , Nurhasanah Nurhasanah , Nupus Hayatun 
Published: February 2020

Penelitian ini bertujuan untuk pengembangan APE berbasis lingkungan pada tema lingkungan untuk pembelajaran saintifik bagi guru-guru korban gempa di Kecamatan Tanjung Kabupaten Lombok Utara. Penelitian ini adalah penelitian action research. Populasi dari penelitian ini yakni seluruh guru korban gempa yang ada di Kecamatan Tanjung dan Sampel penelitian ini yakni 20% dari jumlah populasi yang ada yang akan dipilih secara random yakni sebanyak tiga puluh delapan orang guru. Teknik pengumpulan data menggunakan metode pengisian angket, FGD (Fokus Group Diskusi) dan dokumen. Data yang diperoleh dianalisis menggunakan metode analisis deskriptif kualitatif. Hasil penelitian ini adalah terbentuknya model pengembangan APE berbasis lingkungan untuk pembelajaran saintifik pada tema lingkungan dapat dilakukan dengan beberapa tahapan yakni identifikasi sumber bahan pembuatan APE berbasis lingkungan untuk pembelajaran saintifik pada tema lingkungan, penglompokkan bahan pembuatan APE berbasis lingkungan untuk pembelajaran saintifik pada tema lingkungan, mengembangkan APE berdasarkan pengelompokkan, dan tercipta APE untuk pembelajaran saintifik pada tema lingkungan. Bentuk-bentuk APE yang dapat dikembangkan untuk pembelajaran saintifik yakni bentuk miniatur rumah, bentuk perahu, bentuk mobil-mobilan, bentuk orang-orangan dari pelepah pisang, kertas dan kardus bekas, bentuk ikan dari kertas origami dan bentuk pohon angka dari ranting pohon kering dan angka berbentuk buah apel yang diberi tulisan angka. Dan kelompok-kelompok APE dibagi menjadi dua kelompok APE dengan jenis main peran mikro dan jenis main pembangunan. Dari hasil penelitian ini guru dapat memanfaatkan bahan-bahan yang ada dilingkungan sekitar untuk membuat APE dan dapat menjadi acuan bagi guru dalam meningkatkan kreatifitas dalam mengembangkan APE. Kata kunci: APE, Pembelajaran Saintifik
Dampak Menerapkan Media Pembelajaran Interaktif Calisbar terhadap Prestasi Belajar Siswa
Authors: Pramesti Kadek Mira B. , Agustini Ketut , Santyadiputra Gede Saindra 
Published: February 2020

Tujuan penelitian ini adalah: (1) untuk mengetahui perbedaan prestasi belajar siswa yang belajar menggunakan media Pembelajaran Interaktif CALISBAR dengan siswa yang belajar menggunakan media LKA (Lembar Kerja Anak) pada tema Diri Sendiri di TK Kartika VII-3 Singaraja (2) mengetahui respon siswa setelah menggunakan media Pembelajaran Interaktif CALISBAR dengan tema Diri Sendiri. Jenis penelitian ini adalah eksperimen semu (quasi eksperimen) dengan desain Post Test Only Control Group Design. Populasi penelitian mencangkup seluruh siswa kelompok B di TK Kartika VII-3 Singaraja. Sampel yang digunakan yaitu kelompok B1 sebagai kelas eksperimen dan kelompok B2 yang digunakan kelas kontrol, dengan jumlah 34 orang. Metode pengumpulan data yang digunakan lembar posttest untuk mengukur prestasi belajar, dan metode angket untuk mengukur respon siswa. Data prestasi belajar kemudian dianalisis dengan melakukan uji prasyarat yaitu uji normalitas, uji homogenitas dan uji-t. Hasil penelitian menunjukkan (1) terdapat prestasi belajar yang lebih tinggi dalam pengaruh penggunaan media Pembelajaran Interaktif CALISBAR. Hasil analisis uji-t memperoleh thitung = 7,964 dan ttabel = 2,352 untuk dk sebesar 32 dengan taraf signifikan 5%, karena thitung ttabel maka H0 ditolak (2) respon siswa terhadap penggunaan media pembelajaran Interaktif CALISBAR didapat persentase hasil akhir respon siswa adalah 81,18% yang tergolong dalam kategori sangat positif.