bluuebunny/crossref_metadata_2025_split huggingface dataset:
Coupling coordination relationship between health resource allocation and regional economic development: an empirical study based on five provinces in eastern China
Authors: Wang Yongqiang , Feng Xiaochen , Chai Yulin , Chen Kexuan , Yang Shilan , Li Wei , Mi Yuqing 
Published: December 2024

BackgroundImproving system coordination is a pivotal strategy and a critical pathway for social governance. Chinese society is currently facing a significant challenge in aligning the allocation of health resources with economic development. Evaluating the level of coordinated development within the system can provide valuable insights to support the construction of a more coordinated China and foster high-quality development.MethodsBased on a systematically constructed indicator framework, our study selected data from five eastern provinces of China to establish a ten-year panel dataset covering the period from 2011 to 2020. The comprehensive evaluation index and the relative development degree were employed to comprehensively evaluate the development level of the system. The coupling coordination degree model was applied to analyze the coupling coordination relationship and spatiotemporal evolution trend of the two systems. Additionally, the fixed effects model was used to identify the driving factors behind the coordinated development of the two systems.ResultsFrom 2011 to 2020, the comprehensive indices of health resource allocation and economic development in the five eastern provinces of China exhibited a consistent year-on-year increase, and the relative development degree experienced two critical values of 0.8 and 1.2, which changed from the lagging allocation of health resources to the lagging economic development. The system coordination index generally ranged between 0.35 and 0.90, with the coordination phase undergoing a transition from an antagonistic stage to a coordinated stage. The coordination type also gradually shifted from mild imbalance to good coordination. Furthermore, the levels of economic development, economic structure, technological investment, as well as the allocation of health human and material resources, all serve as critical drivers in enhancing the coordinated development of the system.ConclusionThe coordinated development of eastern China’s provinces produces substantial spillover effects, and the realization of a Healthy China initiative must strategically harness their radiative and demonstrative effects. Achieving a superior level of coordination requires urgent efforts to rectify the existing deficiencies in the distribution of grassroots healthcare resources. Furthermore, cultivating innovative drivers of economic growth and enhancing the capacity for economic support are critical to ensuring high-quality and sustainable development.
Physical activity and its benefits on adolescents' mental health through self-esteem
Authors: Laurier Catherine , Pascuzzo Katherine , Jubinville Vicky , Lemieux Annie 
Published: December 2024

BackgroundSince the onset of the COVID-19 pandemic, the scientific community has been concerned about the high rates of psychological distress among adolescents. The pandemic not only tested adolescents’ adaptation, but also disrupted key areas of their development. This demonstrates the need to study their psychological adjustment over time during this critical period to better guide services.ObjectiveThis study sought to explore the extent to which physical activity and its association with self-esteem in the first months of the pandemic impacted adolescents' psychological distress, six months later.MethodsTwo hundred and ninety-four adolescents (73% girls) between the ages of 12 and 17 participated in a longitudinal study launched at the start of the COVID-19 pandemic. The number of hours spent engaging in physical activity (HPA) in the past week and self-esteem were measured at Time 1 (T1; summer 2020). Psychological distress was measured at T1 and Time 2 (T2; winter 2021).ResultsMore HPA in the past week were related to greater self-esteem at T1. Greater self-esteem at T1 was related to lower psychological distress, six months later (T2). Lastly, HPA in the past week was not directly linked to psychological distress at T2, which confirms a fully indirect model.ConclusionResults suggest that physical activity is a key factor in promoting better mental health adjustment through its benefits in terms of self-esteem, even during times of turmoil. Findings reinforce the recommendation of promoting the practice of sports and athletic activity in difficult times.
Impact of major emergencies on zero hunger, achieving food security, and improving nutrition
Authors: Fang Zhong , Xiao Qiqi , Gao Yuan 
Published: December 2024

IntroductionFood security is a common livelihood issue that has received much attention from countries all over the world. Thus, researching the impact of COVID-19 on eradicating hunger, achieving food security, and improving nutrition can provide experiences for effectively responding to future emergencies that may affect food security.Research and methodsPrevious studies on the impact of COVID-19 on food security are less concerned with its impact on improving nutrition, therefore, this paper opens the black box of the process of eliminating hunger, reaching food security and improving nutrition, and divides it into the agricultural production sub-stage and the elimination of hunger and improvement of nutritional sustainability sub-stage. On the other hand, most of the past studies are based on impact pathway analysis only, and lack the assessment of the degree of impact from a quantitative perspective. Therefore, this paper takes the data of 29 provinces in China from 2016 to 2020 as the object of the study to measure their total and sub-stage efficiencies in the process of eradicating hunger, reaching food security, and improving nutrition, and to explore the characteristics of their spatial distributions. Meanwhile, the focus is concentrated on the 2019–2020 window to explore the impact of COVID-19.Result and discussionBased on research findings, this paper puts forward policy recommendations such as establishing a sound natural disaster prevention and control mechanism and accelerating agricultural technological innovation in order to better reduce the negative impact of food security emergencies in the future.
A novel technique to characterize procoagulant platelet formation and evaluate platelet procoagulant tendency in cats by flow cytometry
Authors: Shaverdian Meg , Nguyen Nghi , Li Ronald H. L. 
Published: December 2024

IntroductionCardiogenic arterial thromboembolism (CATE) is a life-threatening complication of hypertrophic cardiomyopathy (HCM) with a high mortality rate. As the primary responders in hemostasis, platelets play a crucial role in the progression of CATE. Procoagulant platelets are a subpopulation of activated platelets that facilitate thrombin generation to strengthen thrombus structure. Since their discovery, procoagulant platelets have gained a considerable amount of attention due to their potential role in thromboembolic complications. Uncovering the key phenotypic markers and agonists underlying the procoagulant response in feline platelets may provide diagnostic and therapeutic insights in the treatment of CATE. However, species differences in platelet physiology and the sensitive nature of feline platelets pose some significant challenges in studying procoagulant platelets in cats.ObjectivesTo first devise a flow cytometric method to sequentially assess procoagulant platelet markers and to identify agonists that could induce procoagulant platelet phenotypes. Furthermore, a novel scoring system was established to evaluate the procoagulant tendency of platelets in cats.MethodsPlatelets were isolated from eight healthy cats and activated by thrombin in the presence or absence of collagen (COL) and convulxin (CVX). The following markers were measured sequentially by flow cytometry: (1) loss of mitochondrial membrane potential (ΔΨm), (2) phosphatidylserine (PS) externalization, and (3) P-selectin upregulation.ResultsThrombin in the presence of CVX significantly reduced ΔΨm and induced P-selectin upregulation (p = 0.0078, p = 0.0128, respectively). In addition, thrombin-treated platelets in the presence of COL and CVX augmented PS externalization significantly (p = 0.043, p = 0.0172, respectively). Of the agonists tested, thrombin and CVX resulted in the highest procoagulant tendency score with 75% cats reaching a score ≥2/3. The number of cats with a procoagulant tendency score of 2 or 3 for thrombin and thrombin + COL was 4/8 (50%) and 5/8 (62.5%), respectively.DiscussionSequential analysis of procoagulant markers via flow cytometry may be an effective technique for studying procoagulant platelets in cats.
Software Level Security and Privacy Attacks and Challenges in Healthcare Systems
Authors: Memon Sumaira , Memon Shahzad , Dhomeja Lachhmand Das , Memon Bisharat Rasool , Memon Nisar Ahmed 
Published: December 2024

The widespread adoption of electronic healthcare technology holds immerse promise for improving clinical outcomes and transforming healthcare delivery. The COVID-19 pandemic further accelerated the use of Internet of things (IoT), Cloud Computing, Robotics and Artificial Intelligence (AI) for pandemic data analysis, remote diagnostics, use of wireless medical devices and public information services. This surge in usage and integration of smart devices, often connected through healthcare apps has also introduced new cyber-security and concerns. The vast amount of personal data, including health information, makes healthcare systems a prime target for cybercriminals who could exploit it for harassment and fraudulent purposes. Additionally, the increased reliance on anytime, anywhere connectivity creates vulnerabilities in wireless medical devices. Recent reports highlight these threats, with examples including privacy breaches, ransomware attacks, and disruptions in communication channels for medical devices, Such security breaches can erode patient trust, cripple healthcare systems and even endanger lives. to facilitate users also increases various cyber threats. In recent times, privacy attacks on This paper reviews the recent software-level security and privacy threats in smart healthcare systems, explores mitigation techniques proposed in the literature, and discuss the future challenges of software security in smart healthcare.
Morpho-Physiological Responses of Shade-Loving Fern Polystichum spp. to Single and Combined Lead and Light Stress
Authors: Pietrak Anna , Łopusiewicz Łukasz , Gałczyńska Małgorzata , Salachna Piotr 
Published: December 2024

The effects of lead (Pb) stress on plant growth and physiological processes may depend on other environmental stresses coinciding. Knowledge of the response of shade-loving plants to stresses, particularly the relationship between Pb stress and light stress, is lacking. The effects of single and combined Pb and light stress on the growth and physiological parameters of Polystichum setiferum and Polystichum setiferum ‘Proliferum’ ferns were evaluated under glasshouse conditions. Treatments comprised control (80% shade, ~111 μmol m−2 s−1 photosynthetic photon flux density, PPFD), light stress (100% full sunlight, ~525 μmol m−2 s−1 PPFD), 1000 mg dm−3 Pb solution applied to plants under shade and light stress conditions. Under full sunlight, plants had damaged leaves and reduced leaf biomass, and underground parts of the plants had levels of photosynthetic pigments, reducing sugars and total flavonoids. The Pb stress decreased plant growth, reducing sugars, and free amino acids content, and at the same time increased chlorophyll content in P. setiferum and total polyphenols and flavonoid content in P. setiferum ‘Proliferum’. The combined stress of Pb and full sunlight reduced plant growth and the accumulation of pigments, reducing sugars, and free amino acids without affecting the levels of secondary metabolites. P. setiferum plants accumulated more Pb than P. setiferum ‘Proliferum.’ The fern P. setiferum ‘Proliferum’ was more tolerant to abiotic stresses than the fern P. setiferum. This study provided new insights into the response of shade-loving ornamental plants to single and combined Pb and light stress.
Testing of the Taxonomic Diagnosis of Zebrus pallaoroi Kovačić, Šanda & Vukić, 2021 (Actinopteri: Gobiiformes: Gobiidae), on a Large Sample from the Western Mediterranean
Authors: Kovačić Marcelo , Bérenger Lucas , Renoult Julien P. 
Published: December 2024

A large sample of 65 individuals of the recently described goby Zebrus pallaoroi was collected in France. The species identity of these individuals was confirmed based on morphology. In addition, the species identity was validated through genetic analysis for one of the two specimens collected from the new depth records for the species. The diagnostic characters of Z. pallaoroi, originally based on a limited number of type specimens, were tested on this larger sample and critically analyzed. The diagnostic characters of Z. pallaoroi were revised, and recommendations were made for improving the study of diagnostic characters in gobiid species description, particularly when based on small sample sizes. The record of Z. pallaoroi in the western Mediterranean significantly expands its known geographic range, increases the maximum recorded depth of the species, and reveals syntopic co-occurrence with its phylogenetically closest relatives.
Efficient Production of Recombinant Human Brain-Derived Neurotrophic Factor in Escherichia coli Through the Engineering of Its Pro-Region
Authors: Spaccapaniccia Elisa , Cazzorla Tiziano , Rossetti Daniela , De Simone Lucio , Antonangeli Maria Irene , Antonosante Andrea , Galli Francesca , Cattani Franca , Maffei Mariano , Martin Franck 
Published: December 2024

Thus far, no manufacturing process able to support industrialization has been reported for the recombinant human brain-derived neurotrophic factor (rhBDNF). Here, we described the setup of a new protocol for its production in Escherichia coli (E. coli) and its purification to homogeneity. A synthetic gene, codifying for the neurotrophin precursor, was inserted into an E. coli expression vector and transformed into BL21 (DE3) strain. The recombinant protein accumulates, at high yields, into inclusion bodies. With the developed strategy, more than 50% of the precursor can be refolded. The protein is successively digested by trypsin and the rhBDNF mature form is finally purified by two additional chromatographic steps If the wild-type precursor can be efficiently obtained by the proposed methodology, its pro-peptide remotion, through enzymatic digestion, is however problematic. To circumvent this difficulty, the precursor hinge region, containing the natural furin recognition site, was engineered to be more specifically cleaved by trypsin. Notwithstanding the substitution of three residues in the pro-region carboxyterminal, the precursor correctly refolds and is efficiently cleaved to generate a biologically active mature rhBDNF. This efficient high-yield process fills the current need of a scalable protocol to produce GMP-grade material and unlocks the rhBDNF employment in future clinical investigations.
Mortalidade por câncer de colo do útero em Roraima: uma análise epidemiológica de 2012 – 2022
Authors: Santana Ana Clara Menezes de , Nishimuta Flavia Naomi Campoi , Vasconcelos Arthur Medeiros de , Nascimento Kelly Raphaelle de Luna Aguiar , Rocha Marcos Vinicius Veras da 
Published: December 2024

O câncer está entre as principais doenças crônicas responsáveis por mortes prematuras em vários países, sendo uma grave questão de saúde pública, principalmente antes da sétima década de vida. Nessa perspectiva, na região Norte do Brasil, o câncer de colo do útero está classificado como o segundo mais incidente entre as neoplasias malignas, com destaque ao estado de Roraima.
ПРИНЦИПЫ И МЕТОДЫ СЕЛЕКЦИОННОЙОЦЕНКИИОТБОРА ПАТОГЕННОУСТОЙЧИВЫХ ДРЕВЕСНЫХ РАСТЕНИЙ В ЛЕСОРАЗВЕДЕНИИЗАСУШЛИВОГО РЕГИОНА
Authors: Кузнецова Т.В. , Семенютина А.В. , Крюкова Е.А. 
Published: December 2024

В статье рассмотрены основные болезни ильмо вых пород древесных растений и дуба, дано подроб ное описание биологических особенностей морфо логических форм. Определены виды и гибриды древесных расте ний, наиболее устойчивые к специфическим пато генам. Дана сравнительная характеристика устой чивости к графиозу видового разнообразия вяза. Представлены результаты проведенного фито патологического мониторинга плантации вегета тивного потомства и семенного участка на устой чивость к голландской болезни. В статье представлены наиболее агрессивные штаммы возбудителя графиоза. В результате исследований разработаны прин ципы оценки и отбора устойчивых к микозам дре весных растений. Дана оценка устойчивости по би охимическим признакам. Выделены виды и формы с высокой степенью патогеноустойчивости, рекомендуемые для произ водственных целей в лесоразведении: защитные лесные насаждения и урбоэкосистемы.
The article considers the main diseases of elm species of woody plants and oak and gives a detailed description of the biological features of morphological forms. The species and hybrids of woody plants, which are the most resistant to specific pathogens, were determined. Comparative characteristics of resistance to grafiosis (Dutch elm disease) of elm species diversity was given. The results of phytopathological monitoring of the plantation of vegetative offspring and seed area to resistance to Dutch elm disease are presented. The article presents the most aggressive strains of the grafiosis causative agent. As a result of the research, the principles of evaluation and selection of resistant to mycosis woody plants have been developed. The estimation of resistance according to biochemical signs is carried out. The types and forms with a high degree of pathogen resistance, recommended for production purposes in afforestation (protective forest planta tions and urban ecosystems) is selected.
О региональном рынке семян озимой пшеницы в Волгоградской области
Authors: Сухарева Е.П. , Беликина А.В. 
Published: December 2024

В статье рассмотрен рынок семян озимой пше- ницы в Волгоградской области. Определено, что спрос на семена озимой пшеницы неэластичен по цене. Спрос на семена озимой пшеницы изменяется на 5, при изменении на них цены. Предложение эластично по цене, то есть если цена изменяется на процент, то предложение изменяется на 20,3. На рынке семян озимой пшеницы в Волгоград- ской области действует несколько селекционных центров. Рассчитано, что первенство по распро- странению сортов имеет ФГБНУ Аграрный на- учный центр Донской с объемом производства семян всех репродукций 44,4, второе место по концентрации семян озимой пшеницы на рынке за- нимает ФГБНУ НИИСХ Юго-Востока (18,8), третье место ФНЦ агроэкологии РАН (16,1). Рынок се- мян озимой пшеницы можно охарактеризовать как монополистическую конкуренцию, когда на рынке присутствуют несколько продавцов и покупателей, но в меньшем количестве, чем при совершенной конкуренции.
The article considers the market of winter wheat seeds in the Volgograd region. It was determined that consumer demand for winter wheat seeds are not elastic in price. Demand for winter wheat seeds changes by 5, with a change in prices. The offer is elastic in price, e.g. if the price changes by a percentage, the offer changes by 20.3.There are several breeding centers within the winter wheat seed market in the Volgograd Region. It is estimated that the Donskoy Agricultural Research Center (Federal State Budgetary Scientific Institution) has the largest distribution of the sorts with a volume of seeds production of all reproductions of 44.4 the second place (18.8) is occupied by the Federal State Budget Scientific Institution of Agriculture of the South-East according to winter wheat seed concentration within the market, the third place (16.1) is occupied by FNC Agroecology RAS. The winter wheat seed market can be described as monopolistic competition, when there are several sellers and buyers within the market, but those are in smaller amount than in case of a perfect competition.
УРАВНЕНИЯ ВОДНОГО БАЛАНСА ТАЛЫХ ВОД ЛЕСОМЕЛИОРИРОВАННЫХ АГРОЦЕНОЗОВ ДЛЯ МОДЕЛИРОВАНИЯ И УПРАВЛЕНИЯ ЭРОЗИОННО-ГИДРОЛОГИЧЕСКИМИ ПРОЦЕССАМИ
Authors: Панов В.И. 
Published: December 2024

Степной пояс России, включающий в себя ле- состепь, степь и сухую степь, обладает высоким природно-ресурсным потенциалом для успешно- го ведения сельскохозяйственного производства, однако здесь проявляется хронический дефицит влаги с частыми засухами, приводящими к значи- тельной и даже полной потере урожая. Перед сель- скохозяйственной наукой стоит задача повышения биопродуктивности и устойчивости ведения сель- ского хозяйства. В работе рассматривается водный баланс с пози- ции увеличения приходной части и рационального использования расходной части. Показаны результаты теоретических и экспери- ментальных исследований по использованию урав- нений водного баланса талых вод незащищённых и лесомелиорированных агроценозов в качестве алгоритмов (моделей) связи приходно-расходных элементов баланса и применения ландшафтных кластеров (защитно-мелиоративного степного ле- соразведения) воздействия на них для целенаправ- ленного управления эрозионно-гидрологическими процессами.
The steppe zone of Russia, which includes forest-steppe, steppe and dry steppe, has a high natural - resource potential for successful agricultural production, but there is a chronic shortage of moisture with frequent droughts, leading to significant and even complete loss of harvest. Agricultural science faces the challenge of increasing bio-productivity and agriculture sustainability. The water balance is considered from the position of increasing the input part and the rational use of the consumable part. It shows the results of theoretical and experimental studies in terms of the use of the equations of water balance of meltwater of unprotected and forest-reclaimed agrocenoses as algorithms (models) of the connection of input and output the elements of the balance and the use of landscape clusters (protective-meliorative steppe afforestation), and the influence on them for purposeful management of erosion and hydrological processes.