bluuebunny/crossref_metadata_2025_split huggingface dataset:
Проблема оценки эквивалентности компьютерных и традиционных версий психодиагностических методик
Authors: Гнедых Дарья С. 
Published: May 2020

Introduction. This paper (a) discusses the theoretical and methodological evidence for the equivalence of computerized and conventional versions of psychological tests, (b) analyzes the studies investigating psychometric parameters of computerized versions of conventional tests, and (c) examines contradictions in approaches to assessing the equivalence of the two test forms. This paper represents a first effort in structuring the main problems in establishing the equivalence of paper-and-pencil and computer-based testing, as well as in finding ways and means to overcome them. Much attention is devoted to minimal and sufficient mathematical and statistical tools for assessing the equivalence of the two test forms. Theoretical Basis. The main problems associated with assessing the equivalence of computerized and conventional tests include the following: (a) the level of cultural and informational competence of respondents, (b) anxiety, (c) social environment, (d) motivation for testing, (e) difficulties in creating the same conditions for paper-and-pencil and computer-based testing (L. N. Babanin, Y. P. Chua, M. Russell, P. Květon et al.). Researchers most often choose the following procedures for assessing the equivalence of the two versions of psychological tests: (a) comparison of the main statistical parameters (mean values, variances, etc.) and (b) assessment of construct validity and reliability of the computer-based versions of psychological tests. Results and Discussion. The analysis of research methodology for assessing the equivalence of computerized and conventional versions of psychological tests focuses on a variety of approaches to (a) the use of mathematical and statistical methods for assessing psychometric parameters of computer-based versions of conventional tests, (b) the choice of research design, (c) considering specific characteristics of the situation of computer-based testing. It is necessary to formulate specific and structured requirements for the procedure for assessing the equivalence of computerized and conventional versions of psychological tests. The author suggests recommendations for the main sections of such requirements related to (a) the procedure for conducting empirical research, (b) mathematical and statistical methods, and (c) control of the factors specific to computer-based testing that may have impact on the results of equivalence assessment.
Темпоральные особенности студентов как когнитивные диагностические характеристики: контекст адаптивного образования
Authors: Бредун Екатерина В. , Баланёв Дмитрий Ю. , Ваулина Татьяна А. , Краснорядцева Ольга М. , Щеглова Элеонора А. 
Published: May 2020

Introduction. The article presents the results of an experimental study of the individual temporal characteristics of cognitive space management in students while solving problems. Methods. The methodological tools are briefly described, including traditional test questionnaires for identifying temporal features of subjective perception of time (‘Semantic Time Differential’, ‘Time Perspective’), as well as modified and original research methods for identifying temporal characteristics of the cognitive activity of subjects in solving spatial problems in a laboratory experiment (‘Mental rotation’, ‘Accuracy’). Results. In a series of laboratory experiments, new research data were obtained on the temporal specificity of the process of constructing a personal cognitive space in students. Using prognostic modeling procedures, we were able to record the presence of structural relationships between the time perception, cognitive characteristics of problem-solving, and stability of academic performance. It was experimentally proved that characteristics of time reaction and effectiveness in completing accuracy tasks and mental rotation tasks act as temporal predictors of levels of students’ academic performance. Discussion. The study revealed the fact that students with a dominant temporal orientation toward the present situation, looking constantly for new stimuli and feelings in the current time, had quite pronounced deficits in the choice of educational strategies based on more long-term and well-defined objective in the future. It was noted, as an important aspect in the personalization of studying, that students must be aware of their own temporal, cognitive and other characteristics of the information processing, and manage on their own their educational environments as appropriate. The possibilities are discussed in using cognitive diagnostic characteristics to predict and timely correct individual trajectories of students’ educational paths as part of the adaptive approach to education.
Identification of Novel Cytotoxic T Lymphocyte Epitopes of Drug- Resistance Related Protein InhA from Mycobacterium tuberculosis
Authors: Li Dezhi , Dou Zelong , Wu Yahong , Qi Yuanming , Chen Junhui , Gao Yanfeng 
Published: May 2020

Background:Tuberculosis (TB) caused by Mycobacterium tuberculosis (MTB), especially the drug-resistant MTB, poses serious challenges to human healthcare worldwide. Cytotoxic T lymphocytes (CTLs) play a vital role in immune defense against MTB.Objective:To identify novel CTL epitopes that could induce cellular immunity against MTB infections.Methods:The HLA-A*0201 restricted CTL epitopes of the drug-resistant protein InhA from MTB were predicted by online algorisms and synthesized by the Fmoc solid phase method. The candidate peptides were used to induce CTLs from human peripheral blood mononuclear cells (PBMCs) of HLA-A*0201 healthy donors and the HLA-2.1/Kb mice. IFN-γ productions of CTLs were detected by enzyme linked immunospot assay (ELISPOT), flow cytometry and enzyme-linked immunosorbent assay (ELISA), and cytotoxicity was analyzed by lactate dehydrogenase (LDH) assay.Results:A group of 4 epitopes were screened out with high affinities to HLA-A*0201. ELISPOT and flow cytometry analysis indicated these peptides significantly induced that IFN-γ release of CTLs from the HLA-A*0201+/PPD+ donors, as the mutant analogues had more potent stimulation effects. LDH assay showed that CTLs from PPD+ donors and the immunized mice exhibited significant cytotoxicity and low cross-reactivity. ELISA analysis revealed comparative levels of IFN-γ were released by CTLs isolated from the mice spleen.Conclusion:Our study has identified 4 novel CTL epitopes of InhA that could elicit potent CTL immunity, establishing a foundation for the development of multivalent peptide vaccines against the drug-resistant MTB.
Maternal Vitamin D and its Role in Determining Fetal Origins of Mental Health
Authors: Lisi Giulia , Ribolsi Michele , Siracusano Alberto , Niolu Cinzia 
Published: May 2020

:There is evidence that mental health disorders may have roots in fetal life and are associated with deficiencies in various micronutrients, including vitamin D. During pregnancy, vitamin D balance is influenced by an increase in maternal calcitriol and a substantial increase in maternal Vitamin D Binding Protein concentrations. In the early stages of life, vitamin D is necessary to mediate numerous brain processes such as proliferation, apoptosis, and neurotransmission. Furthermore, Vitamin D has a recognized anti-inflammatory activity that normally suppresses inflammation. Increased activation of hypothalamo-pituitary-adrenal axis (HPA) and inflammation during gestation may influence maternal health and fetal neurodevelopment during and beyond pregnancy. A deficit of Vitamin D and maternal stressful events during gestation, such as perinatal depression, could influence the efficacy of the immune system altering its activity.:Vitamin D deficiency during gestation associated with a reduction in fetal brain development has been widely described and correlated with alteration in the production of the brain-derived neurotrophic factor. To this regard, many studies highlights that low maternal vitamin D dosage during gestation has been related to a significantly greater risk to develop schizophrenia and other severe mental illnesses in later life. The objective of this paper is a comprehensive overview of maternal vitamin D balance in determining the fetal origins of mental health with some references to the link between vitamin D levels, inflammatory responses to stress and mental disorders in adult life.
Non-Vitamin K Antagonist Oral Anticoagulants in Pulmonary Embolism: An Overview of Systematic Reviews
Authors: Doundoulakis Ioannis , Antza Christina , Karvounis Haralambos , Giannakoulas George 
Published: May 2020

Background:
Anticoagulation in patients with pulmonary embolism.

Objective:
To identify how non-vitamin K antagonist oral anticoagulants are associated with multiple outcomes in
patients with pulmonary embolism.

Methods:
We performed a systematic search of systematic reviews via multiple electronic databases from inception to August 19th, 2019, without language restriction. Two authors independently extracted data and assessed
the methodological quality of the included systematic reviews using the ROBIS tool.

Results:
We found twelve systematic reviews. Eleven SRs collected their data from randomized clinical trials and
one from observational studies. All the included studies were published between 2014 and 2019 in English. The
methodological quality of the 12 systematic reviews was low to high. None of the systematic reviews, which are
included in our overview of systematic reviews, has evaluated the overall quality of evidence outcome using the
Grading of Recommendations Assessments, Development and Evaluation (GRADE) approach.

Conclusion:
This is the first effort to summarize evidence about non-vitamin K antagonist oral anticoagulants in
an overview of systematic reviews focusing exclusively on patients with pulmonary embolism. The evidence
suggests that the non-vitamin K antagonist oral anticoagulants seem to be more effective and safer than a dualdrug approach with LMWH- VKA.
Effects of Enzymatic Hydrolysis Conditions on the Antioxidant Activity of Red Tilapia (Oreochromis spp.) Viscera Hydrolysates
Authors: Sepúlveda Cindy T. , Zapata José E. 
Published: May 2020

Background:
Fish is an essential source of nutrients for human nutrition due to the composition
of proteins, vitamins, and minerals, among other nutrients. Enzymatic hydrolysis represents an
alternative for the use of by-products of the aquaculture industry.

Objective:
We propose to evaluate the effect of stirring speed, temperature, and initial protein concentration
on the degree of hydrolysis of proteins and antioxidant activity of red tilapia (Oreochromis
spp.) viscera hydrolysates.

Methods:
The effect of stirring speed, temperature, and initial protein concentration on the degree of
hydrolysis of proteins and antioxidant activity was evaluated using an experimental design that was adjusted
to a polynomial equation. The hydrolysate was fractioned to determine the antioxidant activity
of the fractions, and functional properties were also measured.

Results:
Stirring speed and protein concentration presented a statistically significant effect (p <0.05)
on all the response variables. However, the temperature did not present a statistically significant effect
on the degree of hydrolysis.

Discussion:
The best conditions of hydrolysis were stirring speed of 51.44 rpm, a temperature of
59.15°C, and the protein concentration of 10 g L-1. The solubility of the hydrolysate protein was high
at different pH, and the hydrolysate fraction with the highest antioxidant activity has a molecular
weight <1 kDa.

Conclusion:
The degree of hydrolysis and the biological activity of red tilapia viscera hydrolysates
(Oreochromis spp.) are affected by temperature, substrate concentration, and stirring speed. The optimal
conditions of hydrolysis allowed to obtain a hydrolysate with antioxidant activity are due to the
peptides with low molecular weight.
Neoadjuvant Chemotherapy Induces the Appearance of New Copy Number Aberrations in Breast Tumor and is Associated with Metastasis
Authors: Litviakov Nikolai V. , Ibragimova Marina K. , Tsyganov Matvey M. , Doroshenko Artem V. , Garbukov Eugeniy Y. , Slonimskaya Elena M. 
Published: May 2020

Background:
In this study, we examined the CNA-genetic landscape (CNA – copy number
aberration) of breast cancer prior to and following neoadjuvant chemotherapy (NAC) and correlated
changes in the tumor landscape with chemotherapy efficiency as well as metastasis-free survival.

Objective:
Breast cancer patients (n = 30) with luminal B molecular subtypes were treated with anthracycline-
based therapy.

Methods:
To study CNAs in breast tumors, microarray analysis was performed.

Results:
Three effects of NAC on tumor CNA landscape were identified: 1 – the number of
CNA-bearing tumor clones decreased following NAC; 2 – there were no alterations in the
number of CNA-containing clones after NAC; 3 – the treatment with NAC increased the number
of CNA-bearing clones (new clones appeared). All NAC-treated patients who had new tumor
clones with amplification (20%) had a 100% likelihood of metastasis formation. In these cases,
NAC contributed to the emergence of potential metastatic clones. Our study identified the
following loci – 5p, 6p, 7q, 8q, 9p, 10p, 10q22.1, 13q, 16p, 18Chr and 19p – that were amplified
during the treatment with NAC and may be the markers of potential metastatic clones. In other
patients who showed total or partial elimination of CNA-bearing cell clones, no new
amplification clones were observed after NAC, and no evidence of metastases was found with
follow-up for 5 years (р = 0.00000).

Conclusion:
Our data suggest that the main therapeutic result from NAC is the elimination of potential
metastatic clones present in the tumor before treatment. The results showed the necessity of an
intelligent approach to NAC to avoid metastasis stimulation.
Characteristics of Patients with Hypopituitarism having Onset Symptoms of Pseudo-Intestinal Obstruction: A Case Report
Authors: Yuan Ning , Chai Sanbao , Sun Jianbin , Liu Yufang , Xin Sixu , Zhang Xiaomei 
Published: May 2020

Background:
Gastrointestinal symptoms are often the first symptoms of hypopituitarism.
However, pseudo-intestinal obstruction is not a common manifestation of hypopituitarism. Some patients
presenting with gastrointestinal symptoms as their chief complaint were admitted to the Department
of Gastroenterology and were accurately diagnosed with hypopituitarism at the Department of
Endocrinology.

Case Summary:
This case pertains to a 57-year-old man with poor appetite, fatigue, weakness, and
recent onset recurring abdominal pain. An erect, abdominal X-ray indicated flatulence and gas-fluid
levels in the midsection of the abdomen, and pseudo-intestinal obstruction was diagnosed. Subsequently,
the patient was referred to the Department of Gastroenterology to identify the cause of the
pseudo-intestinal obstruction. An examination of the digestive system did not reveal any abnormalities,
but the patient developed hyponatremia and exhibited drowsiness. The patient was transferred to the
Department of Endocrinology for further treatment. The patient was eventually diagnosed with hypopituitarism,
caused by empty sella syndrome. The patient received prednisone and euthyrox replacement
therapy, and pseudo-intestinal obstruction did not occur again.

Conclusion:
In general, endocrine diseases, including hypopituitarism, hypothyroidism, and hyponatremia,
should be considered for patients with pseudo-intestinal obstruction combined with hyponatremia
and drowsiness, especially if the symptoms of the digestive system are not complicated and the
drowsiness is obvious.
Oral Alpha Lipoic Acid Treatment for Symptomatic Diabetic Peripheral Neuropathy: A Randomized Double-Blinded Placebo-Controlled Study
Authors: El-Nahas Mamdouh R. , Elkannishy Ghada , Abdelhafez Hala , Elkhamisy Enas T. , El-Sehrawy Amr A. 
Published: May 2020

Background:
Alpha-lipoic acid (ALA) was used in the treatment of diabetic peripheral neuropathy
(DPN) using different routes, doses and treatment durations. The aim of this work is to assess
the efficacy of oral 600mg ALA twice daily over 6 months in the treatment of patients with DPN.

Methods:
This is a prospective, single-center, double-blinded, placebo-controlled study conducted at
the outpatient clinic of Mansoura Specialized Hospital, Mansoura University. A total of 200 patients
with DPN were randomly assigned to add on treatment with either oral 600mg twice daily ALA
(n=100) or placebo (n=100) for 6 months. Treatment outcome was assessed using vibration perception
threshold (VPT), neurological symptom score (NSS), neurological disability score (NDS), and visual
analog scale (VAS) for pain at baseline and at each visit (1, 3 and 6 months) after the start of treatment.

Results:
Comparison between the study groups regarding the baseline data revealed no statistically
significant differences. with respect to the outcome parameters, no significant differences were found
between the studied groups at baseline. However, in subsequent visits, ALA-treated patients had significantly
better results regarding almost all the outcome parameters (NSS, NDS, VAS, VPT). Mild
nausea was reported in 6 patients. None of the studied patients discontinued treatment.

Conclusions:
Oral 600mg ALA twice-daily treatment for DPN over 6 months is effective, safe and
tolerable.
Association Between sRAGE and Arterial Stiffness in Women with Systemic Lupus Erythematosus
Authors: Wang Hongru , Zeng Yibin , Zheng Huan , Liu Bin 
Published: May 2020

Background:
sRAGE (soluble receptor for advanced glycation end products) is known to
play a protective role in chronic inflammatory diseases, and has been found to be related to arterial
stiffness in hypertensive or diabetic patients. This cross-sectional study was designed to study the potential
association of sRAGE with arterial stiffness in systemic lupus erythematosus(SLE) patients.

Methods:
A total of 94 female SLE patients were enrolled. Brachial-ankle pulse wave velocity
(baPWV) was measured by an automatic pulse wave analyzer. The patients were divided into two
groups according to the baPWV values, those with values greater than 1400cm/s were placed in the
high arterial stiffness group. Biochemical parameters were compared between the two groups. Linear
and logistic regression analysis was used to observe the association between sRAGE and arterial stiffness
in these patients.

Results:
Thirty-five patients were placed in the high arterial stiffness group in which sRAGE levels
were lower (P<0.05). sRAGE levels were significantly related to baPWV(standardized β=1.18,
P<0.01) by linear regression analysis. Multivariate logistic regression analysis showed that sRAGE,
SLE duration, systolic blood pressure, and low-density lipoprotein cholesterol were independent predictors
of arterial stiffness in these patients.

Conclusion:
The results revealed that sRAGE was negatively associated with arterial stiffness in Chinese
female SLE patients.
A NEW VALIDATED THIRD ORDER DERIVATIVE SPECTROSCOPIC METHOD FOR SIMULTANEOUS ESTIMATION OF METOPROLOL SUCCINATE AND RAMIPRIL IN TABLET DOSAGE FORM
Authors: B. ALEKHYA , SINDHUSHA M. , RAUL SORAJ K. , PADHY GOPAL K. 
Published: May 2020

Objective: The objective of the present work is to develop and validate a new UV derivative spectrophotometric method for simultaneous estimation of metoprolol succinate and ramipril in methanol: water (50:50v/v).
Methods: “Zero crossing technique” was chosen for quantitative determination. The zero-crossing points (ZCP’s) were found to be 209 nm where metoprolol succinate was quantified and 211 nm where ramipril was quantified. This method was then subjected to accuracy, linearity, sensitivity and reproducibility according to ICH guidelines to ensure and confirm its validity.
Results: The method was found to be obeying Beer’s law in the range of 10-50 µg/ml and 5-25 µg/ml for metoprolol succinate and ramipril, respectively. The % recoveries were observed between the range of 99.2-100.2 for metoprolol succinate and 99.57-99.86 for ramipril. The intra-day and inter-day results showed reproducibility.
Conclusion: It can be concluded that the developed third-order UV derivative spectroscopic method for the simultaneous determination of metoprolol succinate and ramiprilcan be recommended for routine quantitative analysis.
The THE ROLE OF PLATELET RICH PLASMA AND QUERCETIN IN ALLEVIATING DIMETHYLNITROSAMINE-INDUCED ACUTE SPLEEN INJURY THROUGH REGULATING OXIDATIVE STRESS, INFLAMMATION AND APOPTOSIS
Authors: ZAAZAA ASMAA M. , ANISS NADIA NOBLE-DAOUD 
Published: May 2020

Objective: Excessive oxidative stress is implicated in spleen injury. Platelet-rich plasma (PRP) and quercetin (QUR) have been shown to protect cells against oxidative stress. This study was designed to investigate their effect on dimethyl nitrosamine (DMN) induced spleen injury in male rats.
Methods: Forty male Wistar rats were divided into four groups; Group (1): Negative control group (Con), Group (2): DMN group, DMN was given intraperitonealy at a dose of 4 mg/kg b. wt/day for four weeks for sub-chronic injury of spleen tissue, Group (3): DMN+PRP, rats were injected intraperitonealy with DMN at a dose of 4 mg/kg b. wt/day for four weeks then treated i. v. by single dose 50 μL of PRP, then left for a period of four weeks without any treatments, Group(4): DMN+QUR, rats received intraperitonealy DMN at a dose of 4 mg/kg b. wt/day for four weeks, then treated with quercetin orally at a dose of 50 mg/kg b. wt. in aqueous suspension daily using an intragastric tube for four weeks.
Results: DMN inoculation resulted in significant elevations of oxidative stress, as evidenced by the increased malondialdehyde, hydrogen peroxide and xanthine oxidase levels associated with a significant decrease in Superoxide dismutase and catalase activities in the spleen tissue as compared to the normal control group. Moreover, DMN caused an up-regulation in the values of the splenic C-reactive protein (CRP), interleuckin-6 (IL-6), nuclear factor kappa B (NF-κB), leukotriene-C4 (LT-C4), P53 and Fas levels with a significant decline in anti-apoptotic protein B-cell lymphoma 2 level as compared to the normal control group. PRP and QUR significantly attenuated the DMN-evoked spleen oxidative stress and modulated the activities of antioxidant enzymes as compared to DMN group. In addition, treatment of DMN group with PRP or QUR resulted in an improvement in CRP, IL-6, NF-κB, LT-C4, P53 and Fas levels as compared to DMN group. Caspase-3 expression was positive in DMN group while no difference was present in control, PRP and Quercetin groups. However, the VEGF immunopositive reaction was found in DMN, PRP and Quercetin groups compared to control group. Histopathological results showed degeneration, haemorrhage, inflammatory cells and necrotoic areas in splenic tissue from DMN group compared to the treated groups where signs of recovery were observed in the whole splenic tissue.
Conclusion: These data suggest that PRP and QUR protect rat spleen from DMN-induced oxidative stress, probably via their antioxidant activity, anti-inflammatory and anti-apoptotic effects. So, PRP and QUR are promising pharmacological agents for preventing the potential spleen injury of DMN following occupational or environmental exposures.