bluuebunny/crossref_metadata_2025_split huggingface dataset:
Optimization of scotta as growth medium to preserve biodiversity and maximise bacterial cells concentration of natural starter cultures for Pecorino Romano PDO cheese
Authors: Chessa Luigi , Paba Antonio , Daga Elisabetta , Caredda Marco , Comunian Roberta 
Published: June 2020

ABSTRACT
Preservation of cheese microbiota biodiversity is central to the sensory quality of traditional and PDO cheeses. Lyophilized commercial selected starters, being advantageous in terms of cells concentration, are supplanting natural cultures causing important loss of microbial biodiversity in the dairy environment. Biodiversity could be recovered using natural starter cultures, however their cells concentration after propagation is lower than the commercial ones. Two autochthonous and biodiverse starter cultures (MixA and MixB) coming from scotta (residual whey from Ricotta cheese manufacture), collected in the 1960 s from Pecorino Romano PDO cheese manufactures, were revitalized in reconstituted commercial powder scotta. The aim of this study was the propagation of the microbial starter mixes increasing their bacterial concentration in the pellet, reducing nonessential scotta components by a fast and not-expensive method, without changing the microbial community balance. The behaviour of each mix inoculated in scotta was compared to that in half-concentrated, clarified, and half-concentrated-clarified scotta. Higher cells concentration in the pellets from the modified scotta was obtained, without changing technological performances and microbial fingerprint. The pellets obtained were reinoculated in commercial scotta for the preparation of the scotta-innesto (the typical starter for Pecorino Romano), and no differences were observed among the treatments after incubation. The reduction of nonessential scotta's components could help the reproduction of natural starter cultures preserving their properties.
Peripheral IL-6 Levels but not Sarcopenia Are Predictive of 1-Year Mortality After Hip Fracture in Older Patients
Authors: Bermejo-Bescós Paloma , Martín-Aragón Sagrario , Cruz-Jentoft Alfonso José , Merello de Miguel Ana , Vaquero-Pinto María-Nieves , Sánchez-Castellano Carmen 
Published: June 2020

AbstractBackgroundSarcopenic patients may have an increased risk of poor outcomes after a hip fracture. The objective of this study was to determine whether sarcopenia and a set of biomarkers were potential predictors of 1-year-mortality in older patients after a hip fracture.MethodsAbout 150 patients at least 80 years old were hospitalized for the surgical treatment of a hip fracture. The primary outcome measure was the death in the first year after the hip fracture. Sarcopenia was defined at baseline by having both low muscle mass (bioimpedance analysis) and handgrip and using the updated European Working Group on Sarcopenia in Older People (EWGSOP2) definition of probable sarcopenia. Janssen’s (J) and Masanés (M) cutoff points were used to define low muscle mass.ResultsMortality 1 year after the hip fracture was 11.5%. In univariate analyses, baseline sarcopenia was not associated with mortality, using neither of the muscle mass cutoff points: 5.9% in sarcopenic (J) versus 12.4% in non-sarcopenic participants (p = .694) and 16% in sarcopenic (M) versus 9.6% in non-sarcopenic participants (p = .285). Probable sarcopenia (EWGSOP2) was not associated with mortality. Peripheral levels of IL-6 at baseline were significantly higher in the group of participants who died in the year after the hip fracture (17.14 ± 16.74 vs 11.42 ± 7.99 pg/mL, p = .026). TNF-α peripheral levels had a nonsignificant trend to be higher in participants who died. No other biomarker was associated with mortality.ConclusionsSarcopenia at baseline was not a predictor of 1-year mortality in older patients after a hip fracture. IL-6 was associated with a higher risk of mortality in these patients, regardless of sarcopenia status.
Pathways to Educational Disparities in Disability Incidence: The Contributions of Excess Body Mass Index, Smoking, and Manual Labor Involvement
Authors: Townsend Tarlise , Mehta Neil K 
Published: June 2020

AbstractObjectivesIn the United States, educational disparities in disability are large and increasing, but the mechanisms underlying them are not well understood. We estimate the proportion of population-level educational disparities in disability incidence explained by excess body mass index (BMI), smoking, and manual labor.MethodWe use waves 2003–2015 of the nationally representative Panel Study of Income Dynamics to calculate observed disability incidence and counterfactual incidence absent the key mediators (3,129 individuals; 13,168 observations). We take advantage of earlier-life measures, including childhood socioeconomic status, 1986 BMI, and occupational history between 1968 and 2001. To account for distinct processes in women and men at middle versus older ages, we stratify by gender and at age 65.ResultsEducational disparities in disability incidence were evident in women and men at younger and older ages, and were largest among older women. Together, the mediators of interest were estimated to explain roughly 60% of disparities in younger women, 65%–70% in younger men, 40% in older women, and 20%–60% in older men. The main contributors to disparities appeared to be excess BMI and smoking in younger women; manual labor and smoking in younger men; excess BMI in older women; and smoking in older men.DiscussionThese mediators explain much of disparities in earlier-age disability; successful interventions to address these factors may substantially reduce them. However, a considerable proportion of disparities remained unexplained, particularly at older ages, reflecting the myriad pathways by which educational attainment can influence disability status.
Creative Dislocation: an Experiment in Collaborative Historical Research
Authors: Bickers Robert , Cole Tim , Dudley Marianna , Hanna Erika , McLellan Josie , Pooley William , Williamson Beth 
Published: June 2020

Abstract
This article introduces an experiment in collaborative historical practice. It describes how six historians visited the East Devon village of Branscombe, with the aim of creatively engaging with the present and past of the village. This was a collaborative and collective act of what we term here ‘creative dislocation’. By dislocating from our usual routines, subjects, places, methods, and styles, and adopting creative methods and constraints, we aimed to shed light on the role of creativity in the historical research process. Our experiment resulted in six pieces of writing – three of which are presented here. However, a key argument of this article is that creativity lies in process as much as in the finished product. Creative work happened at each stage of the research process, in ways that were not always immediately visible in the final written pieces. The creativity in historical research and writing does not necessarily lie in opposition to archival explorations and fact-driven narratives, but can also lie within them. Creativity informs the questions we ask, our ways of working with the archive and our approach to writing.
Rheological characteristics of polyethylene-nanotube composites by capillary rheometry
Authors: Al-Baghdadi S , Al-Amiery A 
Published: June 2020

Abstract
The viscosity and flow rate as rheological characteristics are fundamental in evaluating the nanofillers in processing the polyethylene-nanotube (PE-NT) composite in injecting molding. The purpose of this investigation is to study the rheological conduct of PE-NT composite plastic feedstock through capillary rheometry. For the purpose of obtaining a flawless component, the feedstock is used as a critical parameter, and care must be taken while introducing the raw materials with high solid load and hence perfect flowability. The shear rate viscosity of different feedstocks at an NT ratio extending at 0–3 wt.% has been determined at L/D equal to 10 die and a load extending at 40.0–80.0 KGF at temperatures 140.0, 150.0 and 160.0°C. The three specimens’ viscosity was measured in order to exhibit that the corresponding flow conduct factor varies from 0.40 to 0.70, demonstrating the non-Newtonian behavior of the specimens. The three specimens’ activation energies at the studied temperature degrees were evaluated and turned out to be 36.5–69.88 kJ/mol according to the applied load.
Sensitivity and Specificity of Rapid Diagnostic Tests for Hepatitis C Virus With or Without HIV Coinfection: A Multicentre Laboratory Evaluation Study
Authors: Vetter Beatrice N , Reipold Elena Ivanova , Ongarello Stefano , Audu Rosemary , Ige Fehintola A , Alkhazashvili Maia , Chitadze Nazibrola , Vanroye Fien , De Weggheleire Anja , An Sokkab , Fransen Katrien 
Published: June 2020

Abstract

Background
Hepatitis C virus (HCV) screening is critical to HCV elimination efforts. Simplified diagnostics are required for low-resource settings and difficult-to-reach populations. This retrospective study assessed performance of rapid diagnostic tests (RDTs) for detection of HCV antibodies.

Methods
Two lots of 13 RDTs were evaluated at 3 laboratories using archived plasma samples from 4 countries (Nigeria, Georgia, Cambodia, and Belgium). HCV status was determined using 3 reference tests according to a composite algorithm. Sensitivity and specificity were evaluated in HIV-infected and HIV-uninfected populations. Operational characteristics were also assessed.

Results
In total, 1710 samples met inclusion criteria. In HIV-uninfected samples (n = 384), the majority of RDTs had sensitivity ≥98% in 1 or both lots and most RDTs had specificity ≥99%. In HIV-infected samples (n = 264), specificity remained high but sensitivity was markedly lower than in HIV-uninfected samples; only 1 RDT reached &gt;95%. The majority of HIV-infected samples for which sensitivity was low did not have detectable HCV viral load/core antigen. Interreader variability, lot-to-lot variability, and rate of invalid runs were low for all RDTs (&lt;2%).

Conclusions
HCV RDTs should be evaluated in the intended target population, as sensitivity can be impacted by population factors such as HIV status.

Clinical Trials Registration
NCT04033887
A novel community-acquired MRSA clone, USA300-LV/J, uniquely evolved in Japan
Authors: Takadama Shunsuke , Nakaminami Hidemasa , Kaneko Hiroshi , Noguchi Norihisa 
Published: June 2020

Abstract

Background
USA300 [ST8-staphylococcal cassette chromosome mec type IVa (ST8-IVa)/arginine catabolic mobile element (ACME) positive] is a major Panton–Valentine leucocidin (PVL)-positive community-acquired MRSA (CA-MRSA) clone. In Japan, we identified USA300-like strains with characteristics (ST8-IVc/ACME negative) similar to those of USA300.

Objectives
To reveal the evolution of the USA300-like strains.

Methods
The whole-genome sequence of a USA300-like strain was determined and genome analysis was performed using Type Strain Genome Server, MUSCLE and progressiveMauve.

Results
Genome-based phylogenetic analysis showed that the USA300-like strain is more similar to the USA300-Latin American variant (USA300-LV), which is a PVL-positive CA-MRSA clone identified in South America, than to USA300. Instead of the ACME, copper and mercury resistance mobile elements were located on the genome of the USA300-like strain. In addition, the USA300-like strain possessed a unique mobile genetic element, ICE6013. Therefore, we named this novel USA300-LV variant identified in Japan as USA300-LV/J.

Conclusions
Our findings strongly suggest that a PVL-positive CA-MRSA USA300-LV/J clone originating from abroad has uniquely evolved and disseminated in Japan.
Nationwide surveillance of azole-resistant Aspergillus fumigatus environmental isolates in Greece: detection of pan-azole resistance associated with the TR46/Y121F/T289A cyp51A mutation
Authors: Siopi Maria , Rivero-Menendez Olga , Gkotsis Georgios , Panara Anthi , Thomaidis Nikolaos S , Alastruey-Izquierdo Ana , Pournaras Spyros , Meletiadis Joseph 
Published: June 2020

Abstract

Background
Acquired azole resistance (AR) in Aspergillus fumigatus emphasizes the importance of the One Health multisectorial approach. The prevalence of azole-resistant A. fumigatus in the environment of Greece is unknown.

Methods
Between October 2016 and September 2017, a total of 716 soil samples were collected from 23 provinces and screened for AR using azole-containing agar plates. Recovered isolates were macro-/microscopically identified and colonies were counted. Azole susceptibility testing of A. fumigatus species complex (SC) isolates was performed (EUCAST E.DEF9.3.1). Azole-resistant A. fumigatus isolates were subjected to confirmatory molecular identification and sequencing of the cyp51A gene.

Results
No yeasts were recovered, while multiple moulds grew on 695 (97%) samples. Overall, zygomycetes (most non-Mucor genera) grew on 432 (60%) samples, while Aspergillus spp. grew on 500 (70%) [410 (57%) Aspergillus niger SC; 120 (17%) Aspergillus terreus SC; 101 (14%) A. fumigatus SC; 34 (5%) Aspergillus flavus SC]. The mean ± SD soil load of Aspergillus spp. was 2.23 ± 0.41 log10 cfu/g (no differences among species). No azole-resistant non-A. fumigatus spp. isolate was detected. Itraconazole, voriconazole, isavuconazole and posaconazole MIC50/MIC90 (MIC range) of A. fumigatus SC strains were 0.25/0.5 (0.25 to &gt;8), 0.5/1 (0.25 to &gt;8), 1/1 (0.125 to &gt;8) and 0.06/0.125 (0.06–1) mg/L, respectively. Overall, 1/500 (0.2%) of Aspergillus isolates, and 1/101 (1%) of A. fumigatus SC isolates, was pan-azole-resistant (itraconazole, voriconazole, isavuconazole and posaconazole MIC &gt;8, &gt;8, &gt;8 and 1 mg/L, respectively). The resistant isolate was recovered from organically grown raisin grapes treated with homemade compost and it was an A. fumigatus sensu stricto isolate harbouring the TR46/Y121F/T289A mutation. The soil’s load was higher compared with azole-susceptible strains (3.74 versus 2.09 log10 cfu/g).

Conclusions
This is the first known report of environmental pan-azole-resistant A. fumigatus in Greece. Since data on Greek clinical isolates are lacking, this finding must alarm the systematic local surveillance of AR in medical settings.
Telehealth in response to the COVID-19 pandemic: Implications for rural health disparities
Authors: Hirko Kelly A , Kerver Jean M , Ford Sabrina , Szafranski Chelsea , Beckett John , Kitchen Chris , Wendling Andrea L 
Published: June 2020

Abstract
Telehealth programs have long held promise for addressing rural health disparities perpetuated by inadequate healthcare access. The COVID-19 (coronavirus disease 2019) pandemic and accompanying social distancing measures have hastened the implementation of telehealth programs in hospital systems around the globe. Here, we provide specific examples of telehealth efforts that have been implemented in a large rural healthcare system in response to the pandemic, and further describe how the massive shift to telehealth and reliance on virtual connections in these times of social isolation may impact rural health disparities for those without access to necessary broadband to deploy digital technologies. Finally, we provide recommendations for researchers and policymakers to ensure that telehealth initiatives do not amplify existing health disparities experienced by those living in rural communities.
Timing of Flap Surgery in Acute Burn Patients Does Not Affect Complications
Authors: Perrault David , Rochlin Danielle , Pham Christopher , Momeni Arash , Karanas Yvonne , Sheckter Clifford C 
Published: June 2020

Abstract
Pedicled and free flaps are occasionally necessary to reconstruct complex wounds in acute burn patients. Flap coverage has classically been delayed for concern of progressive tissue necrosis and flap failure. We aim to investigate flap complications in primary burn care leveraging national U.S. data. Acute burn patients with known % total body surface area(TBSA) were extracted from the Nationwide/National Inpatient Sample from 2002 to 2014 based on the International Classification of Disease (ICD) codes, ninth edition. Variables included age, sex, race, Elixhauser index, %TBSA, mechanism, inhalation injury, and location of burn. Flap complication was defined by ICD-9 procedure code 86.75, return to the operating room for flap revision. Multivariable analysis evaluated predictors of flap compromise using stepwise logistic regression with backward elimination. The weighted sample included 306,924 encounters of which 526 received a flap (0.17%). About 7.8% of flap encounters sustained electric injury compared to 2.7% of non-flap encounters (odds ratio [OR] 3.76, 95% confidence interval [CI] 1.95–7.24, P &lt; .001). The mean hospital day of the flap procedure was 10.1 (SD 10.7) days. Flap complications occurred in 6.4% of cases. The timing of flap coverage was not associated with complications. The only independent predictor of flap complication was electrical injury (OR 40.49, 95% CI 2.98–550.64, P = .005). Electrical injury was an independent predictor of flap complications compared to other mechanisms. Flap timing was not associated with return to surgery for complications. This suggests that the use of flaps is safe in acute burn care to achieve burn wound closure with an understanding that electrical injuries may warrant particular consideration to avoid failure.
Mortality From Burns Sustained on Home Oxygen Therapy Exceeds Predicted Mortality
Authors: Singer Kathleen E , Harvey Jalen A , Heh Victor , Dale Elizabeth L 
Published: June 2020

Abstract
The Boston Criteria and the Abbreviated Burn Severity Index are two widely accepted models for predicting mortality in burn patients. We aimed to elucidate whether these models are able to predict the risk of mortality in patients who sustain burns while smoking on home oxygen given their clinical fragility. We conducted a retrospective chart review of 48 patients admitted to our burn center from November 2013 to September 2017 who sustained a burn while smoking on home oxygen. Yearlong mortality was the primary outcome of the investigation; secondary outcomes included discharge to facility, length of stay, and need for tracheostomy. We calculated the expected mortality rate for each patient based on Boston Criteria and Abbreviated Burn Severity Index and compared the mortality rate observed in our cohort. Patients in our cohort suffered a 54% mortality rate within a year of injury, compared to a 23.5% mortality predicted by Boston Criteria, which was found to be statistically significant by chi-square analysis (P &lt; .05). Abbreviated Burn Severity Index predicted mortality was 19.7%. While the absolute value of the difference in mortality was greater, this was not significant on chi-square analysis due to sample size. Our secondary outcomes revealed 42% discharge to facility, the average length of stay of 6.2 days, and 6.25% required tracheostomy. Patients whose burns are attributable to smoking on home oxygen may have an increased risk of mortality than prognostication models would suggest. This bears significant clinical impact, particularly regarding family and provider decision making in pursuing aggressive management.
Saturated models of first-order many-valued logics
Authors: Badia Guillermo , Noguera Carles 
Published: June 2020

AbstractThis paper is devoted to the problem of existence of saturated models for first-order many-valued logics. We consider a general notion of type as pairs of sets of formulas in one free variable that express properties that an element of a model should, respectively, satisfy and falsify. By means of an elementary chains construction, we prove that each model can be elementarily extended to a $\kappa $-saturated model, i.e. a model where as many types as possible are realized. In order to prove this theorem we obtain, as by-products, some results on tableaux (understood as pairs of sets of formulas) and their consistency and satisfiability and a generalization of the Tarski–Vaught theorem on unions of elementary chains. Finally, we provide a structural characterization of $\kappa $-saturation in terms of the completion of a diagram representing a certain configuration of models and mappings.
From Which Consumption-Based Asset Pricing Models Can Investors Profit? Evidence from Model-Based Priors
Authors: Kruttli Mathias S 
Published: June 2020

Abstract
This article analyzes whether consumption-based asset pricing models improve the excess returns forecasts of a hypothetical investor with access to these models from 1947 onwards. The investor imposes economic constraints derived from asset pricing models as model-based priors on predictive regression parameters through a Bayesian framework. Three models are considered: habit formation, long-run risk, and prospect theory. The model-based priors generally perform better than priors that shrink the parameter estimates to the historical average model and priors that impose a positive equity premium. This analysis helps to assess the value of consumption-based asset pricing models to investors.
Locally constant constructive functions and connectedness of intervals
Authors: Chernov Viktor 
Published: June 2020

Abstract
We prove that every locally constant constructive function on an interval is in fact a constant function. This answers a question formulated by Andrej Bauer [ 1]. As a related result, we show that an interval consisting of constructive real numbers is in fact connected but can be decomposed into the disjoint union of two sequentially closed nonempty sets.
High-sensitivity observations of molecular lines with the Arecibo Telescope
Authors: Tan W S , Araya E D , Lee L E , Hofner P , Kurtz S , Linz H , Olmi. L 
Published: June 2020

ABSTRACT
We report on one of the highest sensitivity surveys for molecular lines in the frequency range 6.0–7.4 GHz conducted to date. The observations were done with the 305- m Arecibo Telescope toward a sample of 12 intermediate-/high-mass star-forming regions. We searched for a large number of transitions of different molecules, including CH3OH and OH. The low rms noise of our data ($\sim \!5\,$ mJy for most sources and transitions) allowed detection of spectral features that have not been seen in previous lower sensitivity observations of the sources, such as detection of excited OH and 6.7 GHz CH3OH absorption. A review of 6.7 GHz CH3OH detections indicates an association between absorption and radio continuum sources in high-mass star-forming regions, although selection biases in targeted projects and low sensitivity of blind surveys imply incompleteness. Absorption of excited OH transitions was also detected toward three sources. In particular, we confirm a broad 6.035 GHz OH absorption feature in G34.26+0.15 characterized by an asymmetric blueshifted wing indicative of expansion, perhaps a large-scale outflow in this H ii region.