bluuebunny/crossref_metadata_2025_split huggingface dataset:
Stability Analysis of Self Propelled Multi-Utility Platform for Orchard Management System using Point Manipulation Method
Authors: Roul Ajay Kumar , Bhargav Vinod Kumar 
Published: July 2020

Abstract
Self-propelled hydraulically operated multi-utility platform for orchard management system (SOMS) was developed to increase the accessibility of pickers to fruit on trees and to carry out orchard management practices like spraying and pruning. The platform was designed for vertical reach of 6 m and load carrying capacity of 200 kg. Point manipulation programme was developed by Microsoft Excel add in and by trial and error, position of the standard components were decided to determine the centre of gravity of the machine. Standard components were placed over the chassis in such an arrangement that, the centre of gravity (CG) always remain over the chassis during entire operation of the platform from minimum to maximum height with designed load. Forces acting on the chassis were analyzed to find out weight transfer and impending stability in various terrain conditions, various bucket positions loads on the bucket. The prediction equation for the determination of horizontal center of gravity (Xcg) was verified with the true value collected by keeping the wheels of SOMS on individual electronics weighing balance. The average absolute variations between the predicted and measured values of Xcg were within 0.44 % and 3.53%.
miR-765 Impairs Pancreatic β-cell Function by Targeting PDX1 in type 2 Diabetes
Authors: Zheng Li , Wang Yalan , Li Yanhong , Li Li , Wang Xiaohong , Li Yan 
Published: July 2020

Abstract
Background: Type 2 diabetes (T2D) is highly connected with the defect in insulin secretion of pancreatic β-cells, which has been developing into a severe public health problem.Methods: Here, we first detected expression of PDX1 and miR-765 in peripheral blood from 40 patients with T2D and 40 healthy volunteers. INS-1E cells (pancreatic β-cell line) were cultured as experimental model. For glucose induction, we incubated INS-1E cells with 11 mM glucose as control group and INS-1E cells with 25 mM glucose as T2D model group. For target relationship verification, we performed Luciferase reporter assay. Generally, we utilized qRT-PCR (quantitative real-time PCR), western blotting, insulin secretion detection, CCK-8, and flow cytometry in this study.Results: The expression level of PDX1 was dramatically lower in peripheral blood from T2D patients than healthy volunteers, while miR-765 exhibited an opposite result. PDX1 expression level had an inverse correlation with blood glucose level of T2D patients whereas miR-765 exhibited a positive correlation. Furthermore, PDX1 improved insulin secretion, cell viability, and restrained cell apoptosis of INS-1E cells. PDX1 was identified as a target of miR-765 which was observed to reduce insulin secretion, cell viability, and induce cell apoptosis of INS-1E cells.Conclusions: Taken together, we confirmed that miR-765 could cause detrimental effect on pancreatic β-cell survival and function by targeting and repressing PDX1 in T2D.
Influence Psycho-Sexual Factors on The Quality of Life in Pregnant Women During the COVID-19 pandemic: A Path Analysis
Authors: Alijanpour Masoumeh , Sadatmahalleh Shahideh Jahanian , Samaneh Youseflu , Mahnaz Bahri Khomami , Majid Yousefi Afrashteh , Moini Ashraf , Kazemnejad Anoshiravan 
Published: July 2020

Abstract
Background: Coronavirus has spread rapidly around the world. This epidemic has created stress and anxiety for pregnant women in different parts of the world. The aim of this study was to investigate the relationship between quality of life (QoL) with anxiety, depression, corona disease anxiety, sexual function (SF), and marital satisfaction (MS) in pregnant women during the Covid-19 pandemic.Methods: The present study is a cross-sectional study involving 260 pregnant women. The Short-Form Health Survey (SF_12), Marital Satisfaction Scale (MSS), Female Sexual Function Index (FSFI), Hospital Anxiety and Depression Scale (HADS), and coronary disease anxiety questionnaire, Padua Obsession Questionnaire, General Health Questionnaire (GHQ) are used for data collection. Data were analyzed using the Pearson correlation coefficient and path analysis.Result: The overall goodness-of-fit statistics revealed that the predictors of QOL had perfectly good fitness indices (RMSEA=0.02; AGFI=0.99). The results show that corona related anxiety, marital satisfaction (MS), sexual function (SF), depression, and anxiety have a direct effect on women’s QOL. Among variables, GHQ has a more direct effect on women’s QOL. General health (GH) considered as a mediator variable; variables such as anxiety, depression, and Corona-related Anxiety with effect on GH can impress QOL. Anxiety as a main predictor of QOL, with direct, and indirect effects through GH, depression, SF, and MS can impress QOL. Conclusion: Since the QoL in pregnant women is associated with coronavirus epidemics, these results can be used to plan to improve the health and QoL of these people.
Implications of HBV-Driven Epigenetic Remodeling and Its Targets in Liver Cancer Stem Cells
Authors: Demir Ayse Banu , Benvenuto Domenico , Karacicek Bilge , Erac Yasemin , Angeletti Silvia , Ciccozzi Massimo , Tosun Metiner 
Published: July 2020

Abstract
Background: Elevated levels of STIM1, an endoplasmic reticulum Ca2+ sensor/buffering protein, appear to be correlated with poor cancer prognosis where microRNAs are also known to be involved. We investigated a possible viral origin of specific microRNAs identified in Huh-7 human liver cancer stem cells with enhanced STIM1 expression. Methods: Computational strategies including phylogenetic analyses were performed on miRNome data obtained from Huh-7 liver cancer stem cells with enhanced STIM1 and/or Orai1 expression originally cultured in the present study. Results: Results revealed two putative regions in HBV genome based on apparent clustering pattern of stem loop sequences of microRNAs, including miR3653. Reciprocal analysis of these regions revealed critical human genes of which their transcripts are among the predicted targets of miR3653 which was increased significantly by STIM1 enhancement. Conclusion: This study presents a phylogenetic evidence for an HBV-driven epigenetic remodeling to alter gene expression pattern associated with Ca2+ homeostasis in STIM1-overexpressing liver cancer stem cells for a possible mutual survival outcome. A novel region on HBV-X protein may affect liver carcinogenesis in a genotype-dependent manner. Therefore, detection of the HBV genotype would have a clinical impact on prognosis.
Variation in COVID-19 among Eastern Mediterranean Region countries: A comparative study
Authors: Albelbeisi Ahmed Hassan , Albelbeisi Ali , Bilbeisi Abdel Hamid El , Afifi Amany El , Taleb Mahmoud 
Published: July 2020

Abstract
Objective: Coronavirus disease 2019 (COVID-19) is spreads in 216 countries and territories among the world including all Eastern Mediterranean Region countries. The aim of the present study was to examine variation of COVID-19 in different income class countries of the Eastern Mediterranean Region. Methods: Five parameters such as the percentage of variation COVID-19 confirmed cases, the percentage of variation number of deaths, death rate per 1000 confirmed cases, the percentage of variation death rate per 1000 confirmed cases, and tests per one million were calculated. Results: The results demonstrated that all Eastern Mediterranean Region countries increase in percentage of COVID-19 confirmed cases except Tunisia, the increasing range from 53.5% in Libya to 4.7% in Morocco. Death rate per 1000 confirmed cases range from 230.1 in Yemen to 0.92 in Qatar. Majority of countries increase in percentage of COVID-19 deaths cases number, which range from 43.7% in Iraq to 1.4% in Morocco. Fourteen countries decrease in percentage of death rate per 1000, the decreasing range from 34.9% in Libya to 0.09 in Yemen. The tests per one million range from 265687 in United Arab Emirate to four in Yemen. Conclusion: The current study findings showed significant variations between the Eastern Mediterranean Region countries, the substantial variation is concerning. The low-income countries appeared more vulnerable compared to high-income countries; the low-income countries should not be alone with this pandemic. Hence then global should be helping these countries and particular plans have to be employed. These must include screening tests COVID-19 virus.
Identification of Iron Metabolism-Related Genes Signature and Novel Role of FLVCR1 in Hepatocellular Carcinoma
Authors: Chen Jianhui , HU Chuan , Pan Reguang , Du Xuedan , Fu Haotian , Shan Yunfeng 
Published: July 2020

Abstract
Background: Hepatocellular carcinoma (HCC) is the main and highly malignant histological subtype of liver cancer. We tried to construct a novel signature with iron metabolism-related genes to provide new therapeutic targets and improve the prognosis for HCC patients.Methods: The gene expression data of 70 iron metabolism-related genes and its relevant clinical information were obtained from The Cancer Genome Atlas (TCGA) and International Cancer Genome Consortium (ICGC) databases. Consensus clustering analysis was performed to determine clusters of HCC patients with different OS. Cox regression and LASSO regression analyses were used to establish a prognostic signature. Receiver operating characteristic (ROC) and Kaplan–Meier analyses were carried out to examine the predicated performance of the signature.Results: Consensus clustering analysis determined two clusters of HCC patients with different OS(p<0.01), TNM stage(p<0.05) and pathological grade(p<0.05). A nine-gene prognostic signature established with iron metabolism-related genes can independently predicate the prognostic of HCC patients. The ROC curves showed a great performance of the signature. In addition, FLVCR1, a hub gene with the highest mutation frequency in our signature, showed the significantly prognostic value in HCC patients. High FLVCR1 expression was significantly associated with poor prognosis and aggressive progression in HCC patients. The promoter methylation level of FLVCR1 was lower in HCC samples with aggressive progression status. The FLVCR1 expression was positively correlated with the infiltration level of B cell, CD4+ T cell, macrophage, neutrophil and dendritic cell. Conclusion: Our study first established a signature related to iron metabolism and identified FLVCR1 as a potential therapeutic target. These findings provided more treatment strategies for HCC patients.
Wilms’ Tumor 1-Associating Protein Promotes Prostate Cancer Proliferation via Upregulation of CDK4 Transcript
Authors: Zhang Lei , Gao Yiren , Wang Jun , Liang Linghui , Cheng Yifei , Qi Feng , Wang Zengjun , Hua Lixin , Cheng Gong 
Published: July 2020

Abstract
Background: Wilms’ tumor 1-associating protein (WTAP) plays an important role in cell physiological function and have attracted increased interest in cancer research recently. Cyclin-dependent protein kinases (CDKs) are known to participate in regulating the cell cycle and often connected to many malignancies in tumor. We aim to explore whether WTAP or CDKs could play an role in the initiation and progression of prostate cancer(PCa) and hope to provide new insights into PCa treatment and prognostic. Methods: Quantitative real-time PCR, western blotting and immunohistochemistry were performed to explore the expression of WTAP and CDK4 in prostate cancer tissues and cell lines. The survival analysis was used to investigate the association between WTAP expression and the clinical outcomes of prostate cancer. Prostate cancer cell lines were stably transfected with lentivirus approach. CCK-8 assay, colony formation assay, cell invasion and migration assay, cell cycle assay and tumorigenesis in nude mice were performed to study the effect of WTAP in prostate cancer cell lines. RNA immunoprecipitation assay, dual-luciferase reporter assay and siRNA transfection were performed to verify the direct binding sites of WTAP with CDK4 transcript.Results: In prostate cancer tissues and cell lines, WTAP was significantly up-regulated and high expression of WTAP was connected to poor clinical outcomes. Additionally, cell function test indicated that overexpression of WTAP in prostate cancer cell lines could promote cell proliferation, while knocking down showed an opposite results. Subcutaneous xenograft tumor model revealed that overexpression of WTAP could induce tumorigenesis in vivo. Mechanism study showed that CDK4 expression could regulate the expression level of WTAP. Moreover, WTAP could directly bind to 3’-UTR of CDK4 transcript and enhance its stability. Furthermore, specific inhibitors of CDK4 as well as small interfering RNA (siRNA) of CDK4 reversed the promotion of proliferation induced by WTAP.Conclusions: These data indicated that WTAP may act as an oncogenic in prostate cancer by directly binding to CDK4 3’-UTR and stabilizing its transcript which might provide new insights into prostate cancer treatment and prognostic.
A TEORIA ATOR REDE COMO MÉTODO PARA ABORDAGEM SOURCE TO SEA
Authors: Cunha Ellen Joana Nunes Santos , Krelling Allan Paul 
Published: July 2020

O objetivo dessa pesquisa é demonstrar por meio da Teoria Ator Rede os actantes envolvidos na abordagem Source to sea e o mapa de ligações entre os ecossistemas naturais e a proposta Source to sea. O método utilizado foi a Teoria Ator Rede de Bruno Latour, por meio da aplicação de associações rastreáveis para a composição da rede. O estudo permitiu perceber que a abordagem Source to sea possui conexões e interconexões como alianças, fluxos e mediações nas dimensões sociais e ambientais, permitindo a visualização de uma rede. A abordagem Source to sea, preenche uma possível ausência de soluções no entendimento entre os actantes, que tem por base compreender a cooperação transfronteiriça da água e suas possíveis pressões. Conclui-se que a promoção de ações transfronteiriças e locais via abordagem source to sea de gestão para o devido saneamento dos cursos naturais da água, destacam uma grande rede de fluxos nos principais ecossistemas do planeta.
Investigating the Levels of Brain-Specific Proteins in Hydrocephalus Patients
Authors: Guzelcicek Ahmet , Gönel Ataman , Koyuncu Ismail , Cigdem Gulyara , Kose Dogan , Karadag Mehmet , Cadirci Dursun 
Published: July 2020

Background:
Hydrocephalus, a common brain disorder in children, can cause
permanent brain damage. A timely diagnosis of this disorder is crucial.

Objective:
The aim of this study was to evaluate the levels of S-100, CK-18, and NSE brainspecific
proteins in patients with hydrocephalus. We examined the levels of these proteins in the
blood samples of hydrocephalic patients.

Methods:
The study was conducted on the hydrocephalus (n = 31) patients and a healthy control
group (n = 30). A Receiver Operating Characteristic (ROC) curve was used to assess the validity of
the NSE, CK-18, and S100B to differentiate between the hydrocephalus and the control groups.
The suitability of the data to the normal distribution was tested with the Shapiro Wilk test, and the
Student t-test was used to compare the characteristics of the normal distribution in two independent
groups. The individuals in the hydrocephalus and control groups had similar values in terms of age,
height, and weight.

Results:
It was observed that NSE, CK-18, and S100B mean values of the individuals in the
hydrocephalus group were significantly higher than NSE, CK-18, and S100B mean values of the
control group.

Conclusion:
Experiments have shown that the levels of these proteins increase significantly in
hydrocephalus patients compared to the healthy group. These three parameters can be considered
as important markers in the diagnosis of hydrocephalus.
The Effect of Vitamin C Supplementation on Cardiac Enzymes After Coronary Artery Bypass Graft: A Double-blind Randomized Control Trial
Authors: Moludi Jalal , Alizadeh Mohammad , Chehri Godarz , Jafari-Vayghyan Hamed , Foroumandi Elaheh , Maleki Vahid , Ebrahimi Behzad , Sadeghpour Anita , Alizadehasl Azin , Tabaee Ali S. 
Published: July 2020

Background:Coronary artery bypass graft (CABG) is associated with oxidative stress and tissue damage. Vitamin C, as an antioxidant agent, has an important role in attenuating the oxidative stress damage and might have cardio-protective effects after CABG. We aimed to evaluate the probable roles of vitamin C supplementation in cardiac biomarkers after CABG.Methods::In this randomized control trial, 122 patients undergoing CABG were randomly assigned to the intervention (n=54) and control group (n=68) and received vitamin C (2 g intravenously) or placebo, respectively. The surgical methods of the patients in the two groups were identical. The primary efficacy endpoint of this trial is the difference in the levels of CK-MB, Troponin and Total Antioxidant Capacity (TAC) were measured at study entry and 24 hrs after surgery between the two groups.Results::The two groups were not significantly different in terms of basic variables. Within-group comparison showed significant rises in the level of troponin (P < 0.001) and CK-MB (P < 0.001) over time. However, between-group comparison showed no significant difference between the two groups in terms of CK-MB (P=0.826) and troponin (P=0.821). As a whole, the correlation between cardiac enzymes and surgical characteristic was not seen.Conclusion::The results showed that pretreatment with vitamin C could not reduce cardiac marker following CABG. After the intervention, TAC did not differ between and within the intervention and the control groups. Pretreatment with vitamin C as an antioxidant agent could not reduce ischemicreperfusion resulting in CABG.
First Autochthonous case of Emerged Zoonotic Cutaneous Leishmaniasis in Mazandaran Province, Northern Iran: kDNA-PCR Evidence Base
Authors: Niksolat Fatemeh , Tabaripour Rabeeh , Davoodi Lotfollah , Fakhar Mahdi 
Published: July 2020

Background:
In the last decade, several cases of cutaneous leishmaniasis (CL) have
been recorded by the provincial health center of Mazandaran Province, northern Iran. However,
there is no documented report of autochthonous CL in the province yet.

Case Presentation:
The patient, a 59-year-old female, known case of diabetes mellitus, lived in
therural area of Kiasar district (in theeastern part of the Mazandaran) with 5 skin lesions suspected
of having vasculitis, without ahistory of traveling to the endemic areas of CL,was admitted to
Imam Khomeini teaching hospital, Sari. To rule out CL, fine needle aspiration(FNA)procedure was
used aseptically for the patient. Laboratory investigations showed Leishman bodies (amastigotes)
by direct smear preparation, and species identification confirmed Leishmania major (L. major) by
polymerase chain reaction (PCR)molecular method. Accordingly, this is the first documented report
of autochthonous CL in Mazandaran Province.

Conclusion:
Overall, due to the abundance of probable rodent reservoir hosts and confirmation of
infectionof sandflies (Phlebotomus papatasi) withL. major in the Kiasar district, where our patient
was livingthere, it seems that CL emerged in the eastern part of the province. Therefore, further
studies on rodents and sandflies fauna in terms of emerging leishmanial infection in the area is recommended.
Redox Reaction between an Oxo-Bridged Diiron Complex and Hydrazine: A Kinetic Study
Authors: Chandrabanshi Sarvjeet Kumar , Mukherjee Ritam 
Published: July 2020

Objective:
Mechanistic investigation of the reaction between the complex ion
[FeIII
2(μ-O)(phen)4(H2O)2]4+ (1) (phen = 1,10-phenanthroline) and its hydrolytic derivatives
[FeIII
2(μ-O)(phen)4(H2O)(OH)]3+ (1a) and [FeIII
2(μ-O)(phen)4(OH)2]2+ (1b) which coexist in
rapid equilibria in the range pH = 3.00 to pH = 5.00 (pKa1 = 3.71 ± 0.03, pKa2 = 5.28 ±
0.07) with N2H5
+ ion to produce [Fe(phen)3]2+ has been covered in this study.

Methods:
Rise in absorbance of the product with time was measured and rates have been
determined using initial rate methods. Dependence of rate on pH and concentration of
N2H5
+ are studied in detail.

Results:
In the presence of excess phenanthroline, the reaction follows simple first-order
exponential profile. Interestingly, in the absence of any added phenanthroline, the reaction
becomes faster and the reaction profile changes to nearly linear with curvature near the end
of the reaction. Slight autocatalytic nature has been observed only for the slower reactions.
The observed rate constants obtained using initial rate method show first-order dependence
on the concentration of N2H5
+. An increase in rate with increasing pH has been noticed.
The plot of rate versus 1/[H+] shows saturation beginning near pH 4.6, suggesting that the
mono-deprotonated form of the complex (i.e. 1a) is the active oxidant in this pH range.

Conclusion:
Presumably, the reaction becomes faster in the absence of added
phenanthroline because ligand dissociation from the parent complex becomes facilitated
when there is no external phenanthroline ligand present. This produces a coordinatively
unsaturated Fe(III) species which is a faster oxidant.
Preparation and Characterization of scFv-Coupled Immunoaffinity Column for Purification of Fibrinolytic Enzyme from Bacillus subtilis Natto-89
Authors: Choe SunIl , Kim CholJin , Yun UnHui , Li HyonGwang , Kim CholHo 
Published: July 2020

Background:
The focus of this study was to prepare and characterize the single-chain variable
fragment antibody (scFv)-coupled immunoaffinity column for purification of subtilisin BRC.

Methods:
The scFv against subtilisin BRC was immobilized onto CNBr-activated Sepharose 4B. Adsorption
isotherm for subtilisin BRC on scFv-BRC-coupled Sepharose 4B was obtained and calculated
the maximum binding capacity. The extraction conditions, including eluting solution, the concentration
of eluting solution and flow rate, were optimized. Under the optimized eluting conditions, the dynamic
binding capacity of the immunoaffinity column was determined.

Results:
The scFv-BRC-coupled Sepharose 4B for immunoaffinity purification of subtilisin BRC was
prepared. The coupling efficiency was about 78.4%, e.g. about 8 mg of scFv-BRC was covalently coupled
to 1 g CNBr-activated Sepharose 4B. The maximum equilibrium binding capacity (qm) and dissociation
constant (Kd) of the immunoaffinity column for subtilisin BRC were 3.01 mg/mL and 0.465
mg/mL, respectively. The immunoaffinity chromatography conditions were optimized and the subtilisin
BRC was purified 3.29-fold with 55.6%.

Conclusion:
The subtilisin BRC was effectively purified with high purity using scFv-BRC-coupled
Sepharose 4B and the dynamic binding capacity of the column was determined. These results suggested
that scFv-BRC can be used as a ligand for affinity purification of subtilisin BRC.