bluuebunny/crossref_metadata_2025_split huggingface dataset:
The challenge of safe anesthesia in developing countries: defining the problems in a medical center in Cambodia
Authors: Tao Kun-ming , Sokha Sann , Yuan Hong-bin 
Published: March 2020

Abstract
Background: The International Standards for a Safe Practice of Anesthesia (ISSPA) were developed on behalf of the World Federation of Societies of Anaesthesiologists and the World Health Organization. It has been recommend as an assessment tool that allows anesthetic providers in developing countries to assess their compliance and needs. This study was performed to describe the anesthesia service in one main public hospital during an 8-month medical mission in Cambodia and evaluate its anesthetic safety issues according to the ISSPA.Methods: We conduct a retrospective study involving 1953 patients at the Preah Ket Mealea hospital. Patient demographics, anesthetic techniques, and complications were reviewed according to the registers of the anesthetic services and questionnaires. The inadequacies in personnel, facilities, equipment, medications, and conduct of anesthesia drugs were recorded using a checklist based on the ISSPA.Results: A total of 1792 patients received general and regional anesthesia in the operating room, while 161 patients receiving sedation for gastroscopy. The patients’ mean age was 45.0 ± 16.6 years (range, 17–87 years). The three most common surgical procedures were abdominal (52.0%; confidence interval [CI], 49.3–54.7), orthopedic (27.6%; CI, 25.2–29.9), and urological surgery (14.7%; CI, 12.8–16.6). General anesthesia, spinal anesthesia, and brachial plexus block were performed in 54.3% (CI, 51.7–56.8), 28.2% (CI, 25.9–30.5), and 9.4% (CI, 7.9–10.9) of patients, respectively. One death occurred. Twenty-six items related to professional aspects, monitoring, and conduct of anesthesia did not meet the ISSPA-recommended standards. A lack of commonly used drugs and monitoring equipment was noted, posing major threats to the safety of anesthesia practice, especially in emergency situations.Conclusions: This study adds to the scarce literature on anesthesia practice in low- and middle-income countries such as Cambodia. Future medical assistance should help to strengthen these countries’ inadequacies, allowing for the adoption of international standards for the safe practice of anesthesia.
Potential health risk assessment of toxic metals contamination in clay eaten as pica (geophagia) among pregnant women of Ho in the Volta Region of Ghana.
Authors: Kortei Nii Korley , Koryo-Dabrah Alice , Akonor Papa Toah , Manaphraim Nana Yaw Barima , Ayim-Akonor Matilda , Boadi Nathaniel Owusu , Essuman Edward Ken , Tettey Clement 
Published: March 2020

Abstract

Introduction: Geophagia although pleasurable and somewhat a necessity among pregnant women, also comes along with its own attendant problems such as exposure to potentially hazardous substances like bacteria, fungi, helminthes and ova, radioactive materials, and toxic elemental minerals in the soil depending on the geographical location. Methodology: This study evaluated the potential health risk involved during the exposure of pregnant women to toxic elemental minerals via the consumption of clay as pica (geophagia). Elemental mineral analysis was carried out using Buck Scientific 210VGP Flame Atomic Absorption Spectrophotometer (Buck Scientific, Inc. East Norwalk, USA). Risk assessment methods were also used to ascertain the various risks factors and the overall risk level. Results: Concentrations of the macro elements investigated were 1.38 ± 1.5, 2.40± 1.5, 7.74± 1.5, 4.01± 1.0, 13.24± 2.2 and 13.76± 2.1 mg/Kg for Fe, Cu, Zn, K, Mg and Na respectively. While that for the micro elements were 1.63± 0.03 µg/Kg, 4.72±0.8, 0.53±0.02 and 1.85±0.3 mg/kg respectively for Arsenic, Manganese, Lead and Nickel. Estimated Daily Intake (EDI), Hazard Quotient (HQ), Target Hazard Quotient (THQ) and Total Target Hazard Quotient (TTHQ) values ranged 0.611- 5.44 (mg/kg Bw/day), 6.26x10 -4 – 106.5, 0.067-10.34 and 15 respectively. Conclusion: There is the likelihood of posing adverse health problems when clay samples obtained from Anfoega which is sited in the Volta region of Ghana is consumed due to the fact that the calculated HQ’s of these elemental minerals were >1 which points to high content of Manganese (Mn) and Nickel (Ni). It is also likely to cause adverse health problems in an individual’s life time since THQ for Arsenic, Lead and Nickel were above 1. Ultimately, the cumulative effect of these toxicants were exceedingly great (≤ 15) which implied a high level of unsafety associated with this clay. Per the results from this study, it is not safe for pregnant women to consume clay as pica since these toxic elements may cause detrimental effects on the foetus of the unborn child.
The analysis of groundwater nitrate pollution and health risk assessment in Rural Areas of Yantai,China
Authors: Yu Guimei , Wang Jiu , Liu Lei , Li Yun , Zhang Yi , Wang Songsong 
Published: March 2020

Abstract
Background:Nitrate is one of the most common chemical contaminants of groundwater, and it is an important unqualified factor of rural groundwater in Yantai. In order to assess the risk of exposure to drinking water nitrate for adults and juveniles, in recent years, we monitored the nitrate concentrations in rural drinking water,a model was also used to assess the human health risk of nitrate pollution in groundwater. Methods: From the year 2015 to 2018, the drinking water in rural areas of Yantai was tested according to the "Sanitary Standard for Drinking Water" (GB5749-2006). The principal component analysis was used to analyze the relationship between groundwater chemicals and nitrate. The model was used to assess human health risks of groundwater nitrate through the drinking water and skin contact. Results: A total of 2348 samples were tested during the year 2015-2018.Nitrate and total dissolved solids, total hardness, chloride are all relevant, the above indicators may come from the same source of pollution; The median nitrate content (CEXP50) was 17.8 mg / L; the risk of exposure in each group was ranked as: Juveniles > Adult female > Adult male;the median health risk (HQ50) for minors and adults exceed 1. Conclusions:The concentrations of nitrate is stable and does not change over time.The high concentration of nitrate in rural areas of Yantai may be the result of the interaction of fertilizers and geological factors. The risk of exposure to nitrate in juveniles and adults is above the limit, so it is necessary to be on the alert for the high levels of nitrate.
Molecular analysis of lipid uptake- and necroptosis-associated factor expression in vitrified-warmed mouse oocytes
Authors: Um Da-Eun , Shin Hyejin , Park Dayoung , Ahn Jeong Min , Kim Jayeon , Song Haengseok , Lim Hyunjung Jade 
Published: March 2020

Abstract
Background: We had previously demonstrated that vitrification reduces the levels of certain phospholipid classes, and that oocytes from aged mice show a similar lipidome alteration, even without vitrification. In the current investigation, we examined if vitrification-warming of mouse oocytes from young and aged mice causes any changes in molecular aspects of lipid-associated features. Methods: Metaphase II (MII) stage oocytes were harvested from young (10‒14-week-old) and aged (45‒54-week-old) mice by a superovulation regime with PMSG followed by hCG. We examined the status of the intracellular lipid pool and the integrity of the plasma membrane by staining oocytes with BODIPY 500/510 and CellMask live dyes. Expression of lipid uptake- and necroptosis-associated genes was assessed by quantitative PCR analyses, in oocytes from young and old mice, before and after vitrification. Localization patterns of two crucial necroptosis proteins, phosphorylated MLKL (pMLKL) and phosphorylated RIPK1 (pRIPK1) were examined in mouse oocytes by immunofluorescence staining. Necrostain-1 (Nec1), an inhibitor of RIPK1, was used to examine if RIPK1 activity is required to maintain oocyte quality during vitrification. Results: We confirmed that vitrified-warmed oocytes from aged mice showed noticeable decrease in both CellMask and BODIPY 500/510 dyes. Among the lipid uptake-associated genes, C d 36 expression was higher in oocytes from aged mice. Necroptosis is a type of programmed cell death that involves damage to the plasma membrane, eventually resulting in cell rupture. The expression of necroptosis-associated genes did not significantly differ among groups. We observed that localization patterns of pMLKL and pRIPK1 were unique in mouse oocytes, showing association with microtubule organizing centers (MTOCs) and spindle poles. pMLKL was also localized on kinetochores of MII chromosomes. Oocytes treated with Nec1 during vitrification showed a decreased survival rate, indicating the importance of RIPK1 activity in oocyte vitrification. Conclusions: We report that oocytes from aged mice show differential expression of CD36, which suggests that CD36-mediated lipid uptake may be influenced by age. We also show for the first time that pMLKL and pRIPK1 exhibit unique localization pattern in mouse oocytes and this may suggest role(s) for these factors in non-necroptosis-associated cellular processes.
Geographic Information Systems and COVID-19: The Johns Hopkins University Dashboard
Authors: JOAO BELMIRO N 
Published: March 2020

Abstract
Background: This article presents a single case study on the development of a GIS for global monitoring of coronavirus (COVID-19). For such concepts presented about GIS, its use and evolution in epidemic events and a presentation of the context of the current coronavirus outbreak and the meaningless results of consolidating a panel with reliable data.Methods: A single case study of a GIS in continuous development with data sharing and comments from the scientific community was carried out. Because it is not a post-mortem analysis, or a follow-up to a successful case, it was not possible to use more rigorous and systematic approaches such as those used by Lee (1989) and Onsrud, Pinto and Azad (1992) for case studies in GIS.Results: The case study presents the results of the development of a control dashboard, as well as the availability of consolidated data made by researchers at Johns Hopkins University and who showed a reliable platform and a world reference for health comunity.Conclusions: Efforts to develop a dashboard and provide data on the coronavirus outbreak resulted in the immediate replication of several other information systems with different approaches (Power BI, R, Tableau), becoming a reference for any new global epidemic outbreak events.
Influences of club connectedness among young adults in Western Australian community-based sports clubs
Authors: Burns Sharyn , Evans Melissa , Jancey Jonine , Portsmouth Linda , Maycock Bruce 
Published: March 2020

Abstract
Background: Along with physical benefits, community-based sport provides opportunities to enhance connectedness, an important protective factor of social and emotional health. However, young Australians participating in sport have been found to drink alcohol at higher levels than their non-sporting peers, and many clubs serve unhealthy food and beverages. This study explored the association between the dependent variable, level of alcohol consumption (AUDIT-C) and connectedness to club and other health behaviours among young people aged 18-30 years who play club sport in Western Australia.Methods: An online cross sectional survey measured levels of alcohol consumption (AUDIT-C), alcohol-related harm, connectedness (including volunteering and team cohesion), mental wellbeing, healthy food options and club sponsorship among young adults aged 18-30 years involved in sports clubs in Western Australia (n = 242). Relationships and association between the dependent variable (AUDIT-C) and independent variables were assessed.Results: Positive correlations were found between high-risk drinking and club connectedness; team cohesion and club connectedness; mental wellbeing and team cohesion and team cohesion and drinking with team mates and experienced alcohol-related harms. Volunteering was negatively correlated with drinking with team mates and team cohesion. AUDIT-C score was moderately significantly positively correlated with club connectedness (rs = .18, p <.05) and team cohesion (rs = .22, p <.01) Male sportspeople were more likely to drink alcohol at high-risk levels than females (p <.001), and respondents belonging to a club that received alcohol-related sponsorship were more likely to drink at high-risk levels (p = .019). Females were significantly more likely to want healthy food and beverage options provided at their clubs (p = 0.011).Conclusions: Findings from this study may inform policy and practice to enhancing healthy behaviours among young adults participating in community sports clubs in Australia and other countries.
Substances use and its association with sociodemographic, family and environment-related factors among technical and vocational education and training College Students in Ataye, Ethiopia, 2019.
Authors: wubetu abate dargie , Getachew Sintayehu , Negash Wassie 
Published: March 2020

Abstract
Background: substance use is prevalent in Ethiopia. Substance use can have several health problems that are potentially harmful in educational performance, social issues, psychological and physical wellbeing; especially on youth and adolescents. This study aimed to determine the prevalence of lifetime and last one-month substance use and its associated factors among technical and vocational education and training College Students (TVET) in Ataye town. Methods: an institution based cross-sectional study was conducted. Participants were selected by using simple random sampling technique. Chi-square with its p-value was used to identify correlates for each substance use. Bivariate and multivariate binary logistic regression analysis was performed to identify associated factors after controlling cofounding. Odds ratio with their 95% confidence interval, and P < 0.05 was used to declare the significance of associated factors. Results: A total of 483 individuals were participated, which was a response rate of 94%. The prevalence of lifetime substance use was 32.5% (95% CI: 28.2, 36.5). The prevalence of last month's substance use was 21.9% (95% CI= 18.2, 25.5). Among lifetime users, the majority 98(25.5%) were chewed Khat; followed by alcohol drinking 75(19.5%). Cigarettes, cannabis, and cocaine users were 15.3%, 2.5%, and 7.2% respectively. Among last month's substance users, the same proportions (21.9%) chewed Khat; followed by alcohol drinking, (16.6%) and cigarette smoking, (15.3%). Of last month's substance users, 1.2% and 3.3% of students used Cannabis and cocaine respectively. Using multivariate binary logistic regression, Being male (AOR=2.2, 95%CI: 2.3, 3.84), Being from divorced family (AOR=4.1,95%CI: 1.1, 5.8), Living > 20 years in the town (AOR=3.45,95%CI: 1.18,10.1), had family member substance user (AOR=2.5, 95%CI: 1.1, 5.8), had intimate friend substance user (AOR=5.3,95%CI: 2.6,10.9), and availability of substances (AOR=2.3,95%CI: 1.2,4.4.) were independent predictors of lifetime substance use among TVET college students. Conclusions: The prevalence of last month and lifetime substance use among Ataye TVET College students were analogous with other studies done in Ethiopia. Cannabis and cocaine are illegal drugs in Ethiopia. But, the reported proportion of these illegal drug use was significant and needs government attention. Overall, Substance use among adolescents should get further emphasis to lower the prevalence.
Premarital sexual practice and its associated factors in Ethiopia. A systematic review and meta-analysis
Authors: Getaneh Temesgen , Negesse Ayenew , Chane Jemberu 
Published: March 2020

Abstract
Background Globally, large numbers of adolescents engage in premarital sexual practice. Youths who begin early sexual activity are more likely to be exposed for high-risk of having multiple sexual partners, infection with Human Immune Deficiency Virus (HIV) and other sexual transmitted diseases. There are pocket studies which reported meager and fragmented evidences across different parts of Ethiopia related with this issue, hence the aim of this review was also to estimate those meager evidences and come up with concrete information at the national level.Methods articles were retrived through search engines such as: PubMed/MEDLINE, EMBASE, CINAHL, Google Scholar, HINARI portal, and Cochrane Library using the preferred Reporting Items for Systematic Reviews and Meta-Analyses Protocols (PRISMA-P) guidelines. Joanna Briggs Institute Meta-Analysis of Statistics Assessment and Review Instrument (JBI-MAStARI) was used for critical appraisal of the included articles. Random effect model analysis technique was considered to estimate the pooled prevalence of premarital sexual practice with their respective odds ratio (OR) and 95% confidence interval (CI). Cochran’s Q statistic, Egger’s and Begg’s test and meta regression were carried out to assess heterogeneity, publication bias and to identify associated factors respectively.Results Exactly 24 articles with total sample size 14,872 individuals were included for this review. The pooled estimate of premarital sexual practice at the national level was 30.98% (95% CI: 26.74, 35.21). Being female sex (OR=1.55: 95% CI; 1.01, 2.37), age >18yrs (OR=2.39: 95% CI; 1.29, 4.42), being alcohol abuser with (OR=3.47; 95% CI; 1.94, 6.19) and exposed to pornography (OR=3.24:95% CI; 1.95, 5.38) were the associated factors of premarital sexual practice in Ethiopia.Conclusion The pooled prevalence of premarital sexual practice in Ethiopia was high in comparison with other countries. So that it is the strong recommendations of the authors’ to provide an integrated youth’s reproductive and sexual health services on the local context to decrease their exposure for premarital sexual practices and its negative impact on future life opportunities. Keywords: Pooled; Premarital; Sexual; Practice; Prevalence; Ethiopia .
Ethical and social implications of approaching death prediction in humans - when the biology of ageing meets existential issues
Authors: Gaille Marie , Rera Michael , Araneda Marco , Dubost Clément , Guillermain Clémence , Kaakai Sarah , Ricadat Elise , Todd Nicolas 
Published: March 2020

Abstract
Background: The discovery of biomarkers of ageing has led to the development of predictors of impending natural death and has paved the way for personalised estimation of the risk of death in the general population. This study intends to identify the ethical resources available to approach the idea of death as a process and consider the perspective of death prediction. The reflection on human mortality is necessary but not sufficient to face this issue. Knowledge about death anticipation in clinical contexts allows for a better understanding of it. Still, the very notion of prediction and its implications must be clarified. This study outlines in a prospective way issues that call for further investigation in the various fields concerned: ethical, psychological, medical and social. Methods: The study is based on an interdisciplinary approach, a combination of philosophy, clinical psychology, medicine, demography, biology and actuarial science. Results: The present study proposes an understanding of death prediction based on its distinction with the relationship to human mortality and death anticipation, and on the analogy with the implications of genetic testing performed in pre-symptomatic stages of a disease. It leads to the identification of a multi-layered issue, including the individual and personal relationship to death prediction, the potential medical uses of biomarkers of ageing, the social and economic implications of the latter, especially in regard of the way the longevity risk is perceived. Conclusions: The present study work strives to propose a first sketch of what the implications of death prediction as such could be - from an individual, medical and social point of view. Both with anti-ageing medicine and the transhumanist quest for immortality, research on biomarkers of ageing brings back to the forefront crucial ethical matters: should we, as human beings, keep ignoring certain things, primarily the moment of our death, be it an estimation of it? If such knowledge was available, who should be informed about it and how such information should be given? Is it a knowledge that could be socially shared?
Mutations in CD14 gene may have significant effect on mastitis resistance/susceptibility of buffalo.
Authors: Pal Aruna , Pal Abantika , Sharma Arjava , Bhattacharya Tarun kumar 
Published: March 2020

Abstract
Background: CD14 is an important pattern recognition receptor having innate immune function and has antibacterial activity. It binds with LPS of gram-negative bacteria, arachidonic acid, and lipoteichoic acid. Being a receptor, it binds with the pathogen with the help of other cytokines. Mutations in CD14 affect the binding ability which in turn affects the biological potentiality. Method: The present study was conducted on 228 nos. of buffaloes pertaining to four different breeds as Murrah, Mehsana, Surti and Bhadawari. CD14 gene was characterized and polymorphism was detected through Single nucleotide conformation polymorphism. Association study was conducted for different variants of CD14 with mastitis in buffalo, detected through somatic cell count, california mastitis test. Result: Eight variants of CD14 were detected and mutational hotspots were detected in bubaline CD14 with 58 number of non-synonymous SNP, out of which 18 were observed to be deleterious and 34 as thermodynamically unstable. In the present study, we had detected the mutations in CD14 gene and its association with the somatic cell score and other indicators for mastitis. In-silico studies were conducted to understand the molecular mechanism how the mutations affect the biological potentiality by analyzing different domains and structural analysis along with various post-translational modification sites. Conclusion: Deleterious mutations were observed in CD14 gene which have significant effect on mastitis of buffalo. It may be employed for marker assisted selection, therapeutic application of recombinant CD14, gene therapy, transgenic animal production with wild type CD14 resistant to mastitis as future strategy. Keywords: CD14, cytokine, bio-informatics, mutation, SNP, deleterious, I-mutant, Provean, mastitis