bluuebunny/crossref_metadata_2025_split huggingface dataset:
Longitudinal analysis of healthy colon establishes aspirin as a suppressor of cancer-related epigenetic aging
Authors: Noreen Faiza , Chaber-Ciopinska Anna , Regula Jaroslaw , Schär Primo , Truninger Kaspar 
Published: November 2020

Abstract

Background
Colon cancer (CC) is the third most common cancer worldwide, highlighting the importance of developing effective prevention strategies. Accumulating evidence supports that aspirin use reduces CC incidence. We reported previously that aspirin suppresses age-associated and CC-relevant DNA methylation (DNAm) in healthy colon. Here we addressed the aspirin’s effectiveness in longitudinal cohort.

Methods
We measured genome-wide DNAm in 124 healthy normal mucosa samples taken at baseline (time point 1, t1) and after 10-years follow-up (time point 2, t2) from a longitudinal female screening cohort. We investigated the time-dependent methylation drift in aspirin users and nonusers using multivariable regression and related the modulatory effect of aspirin to colonic epigenome-aging and CC.

Results
Over time, compared to nonusers, long-term (≥ 2 years) aspirin users showed less hypermethylated CpGs (proximal: 17% vs. 87%; distal: 16% vs. 70%) and more hypomethylated CpGs (proximal: 83% vs. 13%; distal: 84% vs. 30%). Overall, users showed 2% (P = 0.02) less mean methylation levels than nonusers in proximal colon and displayed repressed methylation age (mAge). Methylation loss in users occurred at several CC-specific tumor suppressors that gained methylation in nonusers. Methylation loss in users effected genes involved in immune system and inflammation, while methylation gain in nonusers effected genes involved in metabolism.

Conclusions
This is the first longitudinal study demonstrating effectiveness of aspirin-use in suppression of age-related and CC-relevant hypermethylation in the normal colon. These findings provide a rationale for future studies to evaluate loci that may serve as markers to identify individuals that will benefit most from aspirin and hence increase its efficiency in CC prevention and therapy.
miR-214-3p-Sufu-GLI1 is a novel regulatory axis controlling inflammatory smooth muscle cell differentiation from stem cells and neointimal hyperplasia
Authors: He Shiping , Yang Feng , Yang Mei , An Weiwei , Maguire Eithne Margaret , Chen Qishan , Xiao Rui , Wu Wei , Zhang Li , Wang Wen , Xiao Qingzhong 
Published: November 2020

Abstract

Background
Inflammatory smooth muscle cells (iSMCs) generated from adventitial stem/progenitor cells (AdSPCs) have been recognised as a new player in cardiovascular disease, and microRNA-214-3p (miR-214-3p) has been implicated in mature vascular SMC functions and neointimal hyperplasia. Here, we attempted to elucidate the functional involvements of miR-214-3p in iSMC differentiation from AdSPCs and unravel the therapeutic potential of miR-214-3p signalling in AdSPCs for injury-induced neointimal hyperplasia.

Methods
The role of miR-214-3p in iSMC differentiation from AdSPCs was evaluated by multiple biochemistry assays. The target of miR-214-3p was identified through binding site mutation and reporter activity analysis. A murine model of injury-induced arterial remodelling and stem cell transplantation was conducted to study the therapeutic potential of miR-214-3p. RT-qPCR analysis was performed to examine the gene expression in healthy and diseased human arteries.

Results
miR-214-3p prevented iSMC differentiation/generation from AdSPCs by restoring sonic hedgehog-glioma-associated oncogene 1 (Shh-GLI1) signalling. Suppressor of fused (Sufu) was identified as a functional target of miR-214-3p during iSMC generation from AdSPCs. Mechanistic studies revealed that miR-214-3p over-expression or Sufu inhibition can promote nuclear accumulation of GLI1 protein in AdSPCs, and the consensus sequence (GACCACCCA) for GLI1 binding within smooth muscle alpha-actin (SMαA) and serum response factor (SRF) gene promoters is required for their respective regulation by miR-214-3p and Sufu. Additionally, Sufu upregulates multiple inflammatory gene expression (IFNγ, IL-6, MCP-1 and S100A4) in iSMCs. In vivo, transfection of miR-214-3p into the injured vessels resulted in the decreased expression level of Sufu, reduced iSMC generation and inhibited neointimal hyperplasia. Importantly, perivascular transplantation of AdSPCs increased neointimal hyperplasia, whereas transplantation of AdSPCs over-expressing miR-214-3p prevented this. Finally, decreased expression of miR-214-3p but increased expression of Sufu was observed in diseased human arteries.

Conclusions
We present a previously unexplored role for miR-214-3p in iSMC differentiation and neointima iSMC hyperplasia and provide new insights into the therapeutic effects of miR-214-3p in vascular disease.
Deep double-pilot-based hybrid precoding in UAV-enabled mmWave massive MIMO
Authors: Hong Ziyao , Li Ting , Li Fei 
Published: November 2020

Abstract
Unmanned aerial vehicle (UAV)-enabled communication system provides flexibility and reliability compared to conventional ones. Millimeter wave (mmWave) and massive multiple-input–multiple-output (MIMO) have widely been researched since recent years, which are promising techniques for the next and even the later generation communication system. Hybrid precoding, as a method to reduce the high cost in hardware and power brought by massive antenna array, develops fiercely and is often combined to deep learning, a kind of popular optimization tool, which brings an overwhelming performance. On the other hand, there are not so many attentions about the hybrid precoding in time-varying mmWave massive MIMO, which is necessary to be considered in a UAV-enabled communication scenario because the performance will degrade seriously if the channel changed while the transmitter and receiver use the precoding matrix corresponding to the expired channel, yet. In this paper, we propose a double-pilot-based hybrid precoding system, which completes analog precoding and digital precoding separately—predicting the previous one using deep learning structure and updating equivalent channel frequently for the post one by enhancing the frequency of equivalent channel estimation.
Newspaper article-based agent control in smart city simulations
Authors: Kim Euhee , Jang Sejun , Li Shuyu , Sung Yunsick 
Published: November 2020

AbstractThe latest research on smart city technologies mainly focuses on utilizing cities’ resources to improve the quality of the lives of citizens. Diverse kinds of control signals from massive systems and devices such as adaptive traffic light systems in smart cities can be collected and utilized. Unfortunately, it is difficult to collect a massive dataset of control signals as doing so in the real-world requires significant effort and time. This paper proposes a deep generative model which integrates a long short-term memory model with generative adversarial network (LSTM-GAN) to generate agent control signals based on the words extracted from newspaper articles to solve the problem of collecting massive signals. The discriminatory network in the LSTM-GAN takes continuous word embedding vectors as inputs generated by a pre-trained Word2Vec model. The agent control signals of sequential actions are simultaneously predicted by the LSTM-GAN in real time. Specifically, to collect the training data of smart city simulations, the LSTM-GAN is trained based on the Corpus of Contemporary American English (COCA) newspaper dataset, which contains 5,317,731 sentences, for a total of 93,626,203 word tokens, from written texts. To verify the proposed method, agent control signals were generated and validated. In the training of the LSTM-GAN, the accuracy of the discriminator converged to 50%. In addition, the losses of the discriminator and the generator converged from 4527.04 and 4527.94 to 2.97 and 1.87, respectively.
Meta-synthesis of qualitative evidence in road traffic injury prevention: a scoping review of qualitative studies (2000 to 2019)
Authors: Azami-Aghdash Saber 
Published: November 2020

AbstractBackgroundA considerable number of qualitative studies have been published in recent years on the issues that the quantitative studies have limitations on. This study aimed at performing a meta-synthesis on qualitative studies on Road Traffic Injuries (RTIs) with a scoping review approach.MethodsThis meta-synthesis study was conducted as a scoping review in 2019. The Arkesy and O’Malley framework was applied which has six steps of identifying the research question, identifying the relevant studies, selecting the studies, charting the data, data analysis and reporting the results, and consultation exercise. The required data were gathered by searching the relevant keywords in databases of PubMed, web of knowledge, Scopus, Cochrane Library, Science Direct, Google scholar, Sid, IranMedex. Extracted data were analyzed by the Content-Analysis method.ResultsFinally, 30 studies were included. Extracted data summarized in five main themes and 17 sub-themes. The main themes were: consequences (individual, family, social, financial), the needs of survivors (social support and healthcare), risk factors (general risk factors, risk factors for motorcyclists, risk factors for children and adolescents), barriers of prevention (general barriers, pre-hospital barriers, emergency, and hospital barriers), and prevention solutions (increasing safety, rules and regulations, education, increasing equipment, scientific solutions) of RTIs.ConclusionThis study combined the methods of the scoping review and the meta-synthesis to mapping all qualitative studies on the RTIs, with this approach, this study provides extensive and practical information for policy-makers, managers, practitioners, and researchers in the field of RTIs. Also, by applying this approach, the gaps in the existing knowledge and areas in need of further research are identified.
Determination of veterinary pharmaceutical runoffs from a swine manure pile using LC–MS/MS
Authors: Park Min-Kyu , Oh Joo-Yeon , Lee Sung-Eun , Choi Sung-Deuk 
Published: November 2020

Abstract
The mass usage of veterinary pharmaceuticals in farms has contributed to environmental pollution in vicinity waters, soils, and sediments from farms and composting facilities. In the present study, we investigated the usage of four antibiotics (viz., lincomycin, sulfamethazine, sulfamethoxazole, and trimethoprim) to understand their contamination routes from livestock manure piles. Residual levels of these antibiotics in a nearby reservoir were set as a positive control (Site 1), and a swine manure pile in a farm (Site 2) and a soil sample around the manure pile (Site 3) were selected for this study. Artificial rainwater was flowed into the manure sample (Site 2), the soil sample around the manure pile (Site 3), and a soil sample around the vicinity river (Site 4). A stream sample (Site 5) around the manure pile and river water near the manure pile (Site 6) were also collected. For qualitative and quantitative analyses, analytical validation was performed, and all the four antibiotics were detected at Site 1 in the concentration range of 0.03–1.6 µg/L. Lincomycin was the antibiotic with the highest detection level. At Site 2, the detection level of all antibiotics remained at 0.3–17.3 µg/L, and their residual amounts were continuously detected in subsequent samples with approximately 30-fold decrease. The migration of antibiotics was confirmed to be independent of pH value. Therefore, this study indicates that farm manure pile should be thoroughly managed for antibiotic contamination in vicinity areas with periodical monitoring, especially waterways.
Spatiotemporal dynamics, risk areas and social determinants of dengue in Northeastern Brazil, 2014–2017: an ecological study
Authors: do Carmo Rodrigo Feliciano , Silva Júnior José Valter Joaquim , Pastor Andre Filipe , de Souza Carlos Dornels Freire 
Published: November 2020

Abstract

Background
Dengue fever is an arthropod-borne viral disease caused by dengue virus (DENV) and transmitted by Aedes mosquitoes. The Northeast region of Brazil is characterized by having one of the highest dengue rates in the country, in addition to being considered the poorest region. Here, we aimed to identify spatial clusters with the highest dengue risk, as well as to analyze the temporal behavior of the incidence rate and the effects of social determinants on the disease transmission dynamic in Northeastern Brazil.

Methods
This is an ecological study carried out with all confirmed cases of dengue in the Northeast Brazil between 2014 and 2017. Data were extracted from the National Notifiable Diseases Information System (SINAN) and the Brazilian Institute of Geography and Statistics (IBGE). Local empirical Bayesian model, Moran statistics and spatial scan statistics were applied. The association between dengue incidence rate and social determinants was tested using Moran’s bivariate correlation.

Results
A total of 509 261 cases of dengue were confirmed in the Northeast during the study period, 53.41% of them were concentrated in Pernambuco and Ceará states. Spatial analysis showed a heterogeneous distribution of dengue cases in the region, with the highest rates in the east coast. Four risk clusters were observed, involving 815 municipalities (45.45%). Moreover, social indicators related to population density, education, income, housing, and social vulnerability showed a spatial correlation with the dengue incidence rate.

Conclusions
This study provides information on the spatial dynamics of dengue in northeastern Brazil and its relationship with social determinants and can be used in the formulation of public health policies to reduce the impact of the disease in vulnerable populations.
Evolving pituitary hormone deficits in primarily isolated GHD: a review and experts’ consensus
Authors: Binder Gerhard , Schnabel Dirk , Reinehr Thomas , Pfäffle Roland , Dörr Helmuth-Günther , Bettendorf Markus , Hauffa Berthold , Woelfle Joachim 
Published: November 2020

AbstractIsolated growth hormone deficiency (GHD) is defined by growth failure in combination with retarded bone age, low serum insulin-like growth factor-1, and insufficient GH peaks in two independent GH stimulation tests. Congenital GHD can present at any age and can be associated with significant malformations of the pituitary-hypothalamic region or the midline of the brain. In rare instances, genetic analysis reveals germline mutations of transcription factors involved in embryogenesis of the pituitary gland and the hypothalamus. Acquired GHD is caused by radiation, inflammation, or tumor growth. In contrast to organic GHD, idiopathic forms are more frequent and remain unexplained.There is a risk of progression from isolated GHD to combined pituitary hormone deficiency (> 5% for the total group), which is clearly increased in children with organic GHD, especially with significant malformation of the pituitary gland. Therefore, it is prudent to exclude additional pituitary hormone deficiencies in the follow-up of children with isolated GHD by clinical and radiological observations and endocrine baseline tests. In contrast to primary disorders of endocrine glands, secondary deficiency is frequently milder in its clinical manifestation. The pituitary hormone deficiencies can develop over time from mild insufficiency to severe deficiency. This review summarizes the current knowledge on diagnostics and therapy of additional pituitary hormone deficits occurring during rhGH treatment in children initially diagnosed with isolated GHD. Although risk factors are known, there are no absolute criteria enabling exclusion of children without any risk of progress to combined pituitary hormone deficiency. Lifelong monitoring of the endocrine function of the pituitary gland is recommended in humans with organic GHD. This paper is the essence of a workshop of pediatric endocrinologists who screened the literature for evidence with respect to evolving pituitary deficits in initially isolated GHD, their diagnosis and treatment.
Transitions in alcohol use over time: a survival analysis
Authors: Koenig Laura B. , Haber Jon Randolph , Jacob Theodore 
Published: November 2020

Abstract

Background
The current study examined the predictors of the onset of alcohol use as well as predictors of remission and relapse, both from heavy drinking and from alcohol dependence. Similarities and differences in both clinical and psychosocial predictors across the transitions were examined.

Methods
A sample of men from the Vietnam Era Twin Registry (N = 1769) completed an assessment of lifetime drinking history, which allowed age markers for starting and stopping different drinking patterns. The men also completed various assessments regarding personality, alcohol motives, and psychiatric diagnoses. Survival analyses were used to examine the predictors of the three transitions of onset, remission, and relapse for the phenotypes of heavy drinking and of alcohol dependence, censoring the individuals who had not yet experienced an event.

Results
As expected, predictors of onset for drinking, heavy drinking, and alcohol dependence were largely consistent and included externalizing symptomology, nicotine dependence, and cotwin history of drinking as risk factors. Predictors of remission from heavy drinking, somewhat similarly to remission from alcohol dependence, included the risk factor of externalizing disorders but also, as predicted, included more risk and protective factors in the psychosocial realm that were not predictors of onset. Contrary to our prediction, relapse to heavy drinking and alcohol dependence were predicted largely by unique psychosocial risk and protective factors including social and coping motives.

Conclusion
Current findings extend the findings of past research to remission and relapse in the later decades of life and have implications for treatment of alcohol use problems.
Coping styles in HIV positives and HIV negatives
Authors: Heydari Mohammadreza , Karimzadeh Yasaman , Faghih Marjan , Heydari Zahra , Hosseini Elhamsadat , Mehraeen Morteza 
Published: November 2020

Abstract

Background
Choosing the most useful and versatile way to solve one's personal and social problems is one of the most important choices in individual life. The aim of this study was to compare the coping styles of people living with Human immunodeficiency virus positive and negative.

Methods
This is a Cross-sectional study that accomplished in Shiraz Behavioural Disease Counselling Centre in 2019 and 2020. For this purpose, in the first phase, 40 HIV+ and 40 HIV− patients were randomly selected to answer the questionnaire of dealing with the stressful conditions of Andler and Parker. In the second phase, the same questionnaire was filled out along with a reality distortion questionnaire from similar individuals (40 HIV+ and 40 HIV−).

Results
92% of the HIV population in this study was between 15 and 55 years and 8% was upper than 55 years. 90% of them had no university degree. Among all, 47.5% of them were, 48.5% were self-employed and 49% of them were infected sexually. The results showed that in the first stage there was a significant relationship between marital status and the chances of getting the disease in people, and after controlling the demographic factors, coping styles did not show a significant effect on the disease. In the second stage, the factors of age, sex, education, and marital status had significant effects on people living with HIV, but the effect of coping styles on people with HIV was not significant (P < 0.05).

Conclusion
Therefore, it can be concluded that demographic factors more than coping styles can affect the chances of high-risk behaviours; so, what is identified and measured as a coping style in people in the process that leads to the manifestation of high-risk behaviours or healthy behaviour does not matter much. It should be noted that the reason for rejecting the hypotheses of this study could be the effect of cultural and social factors of Iranian society.
Coevolution of acoustical communication between obligate avian brood parasites and their hosts
Authors: Wang Jiaojiao , Li Qihong , Yang Canchao 
Published: November 2020

Abstract
The mutually antagonistic processes producing adaptations and counter-adaptations in avian brood parasites and their hosts provide a model system for the study of coevolution; this topic has long been an area of focus in ornithology and evolutionary biology. Although there is an extensive body of literature dealing with avian brood parasitism, few empirical studies have considered the effects of the coevolutionary processes associated with brood parasitism on the acoustic characteristics of parent–offspring communication. Under the strong selection pressures associated with brood parasitism, parasitic birds may, for instance, produce deceptive songs. The host may in turn evolve the ability to recognize these sounds as deceptive. At present, the mechanisms underlying the different competitive strategies employed by hosts and parasitic birds remain unclear. Here, we reviewed previous studies that investigated acoustic traits in scenarios of brood parasitism, highlighting possible adaptive functions. Using a meta-analysis, we identified no heterogeneity among studies of begging call adaptations in parasitic nestlings. However, our results may have been affected by the small number of applicable papers available for analysis. Our meta-analysis also suggested that studies of acoustic communication and transmission in adult hosts were highly heterogenous, suggesting that research methods were inconsistent among studies. Finally, we identified knowledge gaps and proposed several lines of future research.
Bone turnover markers to assess jawbone quality prior to dental implant treatment: a case-control study
Authors: Yasuda Keisuke , Okada Shinsuke , Okazaki Yohei , Hiasa Kyou , Tsuga Kazuhiro , Abe Yasuhiko 
Published: November 2020

Abstract

Background
Bone quality is as important as bone mineral density in terms of bone strength. Bone turnover markers (BTMs) are clinical indicators of bone quality. In implant dentistry, bone quality is considered equivalent to bone density on radiographic assessments. The purpose of this study was to determine whether the BTM values are reflected in jawbone condition by evaluating the relationship at baseline and during follow-up in patients with prosthodontic implants.
Computed tomography (CT) scans were obtained and BTM (osteocalcin, bone-specific alkaline phosphatase, pyridinoline cross-linked carboxyterminal telopeptide of type I collagen, and crosslinked N-telopeptide of type I collagen) levels in blood samples were measured in partially edentulous eighteen patients before implant surgery. During the follow-up observation after implant surgery, marginal bone loss (MBL) was measured on dental radiography. We investigated the relationship between the presence of BTM abnormalities and radiographic bone density.

Results
More women than men had abnormal BTM values. Bone turnover was accelerated in the group of women with abnormal BTM values. The density of cancellous bone at the implant placement site was significantly lower in the patients with abnormally high BTM values than in their counterparts with BTM values in the normal range.

Conclusions
Female patients who undergo implant treatments may have reduced bone quality; evaluations of bone strength will require assessments of both BTMs and the density of cancellous bone.