bluuebunny/crossref_metadata_2025_split huggingface dataset:
hUMSCs Regulate the Differentiation of Ovarian Stromal Cells via TGF-β1/Smad3 Signaling Pathway to Inhibit Ovarian Fibrosis to Repair Ovarian Function in POI Rats
Authors: Cui Linlu , Bao Hongchu , Liu Zhongfeng , Man Xuejing , Liu Hongyuan , Hou Yun , Luo Qianqian , Wang Siyuan , Fu Qiang , Zhang Hongqin 
Published: July 2020

Abstract
Background: The basic pathological changes of primary ovarian insufficiency (POI) include ovarian tissue fibrosis and follicular development disorders. The human umbilical cord mesenchymal stem cells (hUMSCs) transplantation has been shown an effective method to improve the ovarian function in POI rat model; however, the exact mechanisms are still unclear. The purpose of this study is to investigate whether the recovery of ovarian function in POI rats are related to the inhibition of tissue fibrosis following hUMSCs transplantation. Furthermore, the transforming growth factor-β1 (TGF-β1) signaling pathway is explored to determine the mechanisms of ovarian function recovery through its inhibition of tissue fibrosis.Methods: The primary ovarian insufficiency (POI) rat model was established by intraperitoneal injection of chemotherapy drug cisplatin (CDDP) for 7 days, The levels of serum sex hormones were measured using enzyme-linked immunosorbent assay (ELISA). The tissue fibrosis in ovary was examined using masson staining and Sirus red staining. The collagen fibers in the ovarian tissues were detected by Western blot analysis. To investigate the mechanisms of ovarian function recovery following hUMSCs transplantation, ovarian stromal cells were isolated from the ovarian cortex of immature rats. The expression of Cytochrome P450 17A1 (Cyp17a1) and fibrosis marker of alpha smooth muscle Actin (α-SMA) in ovarian stromal cells were examined using immunofluorescence analysis. Also, the protein levels of Cyp17a1 and α-SMA in ovarian stromal cells were examined by western blot analysis. The expression of TGF-β1 and Smade3 signals were measured by Western blot and quantitative reverse-transcription polymerase chain reaction (qRT-PCR) analysis.Results: The results show that the function of ovary in POI rats were significantly improved after hUMSCs transplantation. The expression of fibrosis markers (α-SMA) and production of Collagen Type I (Collagen Ⅰ) and Collagen Type Ⅲ (Collagen Ⅲ) in POI rats was significantly inhibited in POI rats following hUMSCs transplantation. In the cultured ovarian stromal cells, the decrease of TGF-β1 and p-Smade3 protein expression was observed in hUMSCs treated POI rats. The treatment with TGF-β1 inhibitor of SB431542 further confirmed this signal pathway was involved in the process. Conclusion: Our study demonstrated that the TGF-β1/Smad3 signaling pathway was involved in the inhibition of ovarian tissue fibrosis, which contributed to the restoration of ovarian function in POI rats following hUMSCs transplantation.
Life Satisfaction among a Clinical Eating Disorder Population
Authors: Claydon Elizabeth , DeFazio Caterina , Lilly Christa L. , Zullig Keith J. 
Published: July 2020

Abstract
Background
The primary objective was to understand life satisfaction (LS) of patients with eating disorder (ED) diagnoses in relation to eating pathology severity, personal/familial ED history, and key demographic and anthropometric variables. Methods: Participants (N = 60) completed the Satisfaction with Life Scale (SWLS), the Eating Pathology Severity Index (EPSI), and demographic questionnaires. Bivariate associations via correlations and multiple linear regression models were used to explore these relationships.
Results
The average SWLS score was 3.7 out of 7, suggesting below average LS. LS was statistically significantly associated with private insurance, past ED, EPSI muscle building, EPSI restricted eating, and EPSI negative attitudes. When included in multiple linear regression, the model explained 33% of the variability of LS [F (7, 56) = 3.4, p = 0.0054, R2 = 0.33]. EPSI muscle building remained the strongest predictor (β = 0.13, p = 0.04).
Conclusions
Based on the data, individuals who have/have had EDs on average score lower on the SWLS than the general population. Individuals scoring within this range typically experience significant issues in several areas of life or a substantial issue in one area. Findings suggest a need for interventions that focus on improving LS in hopes of simultaneously reducing ED symptoms.
Swimming Exercise Modifies Oxidative Stress in Skeletal and Cardiac Muscles of Diabetic Rats
Authors: Pio Talita Fernanda , Orzari Lucas Eduard , Alves Armindo Antônio , Silveira Paulo Cesar Lock , Esquis Marcelo Augusto Marretto , Andrade Thiago Antônio Moretti de , Mendes Maira Felonato , Dalia Rodrigo Augusto 
Published: July 2020

Abstract
Background: Oxidative stress is a key factor in the worsening of diabetes, requiring an increase in the activity of the antioxidant system, culminating in a balance in the body. The aim of the present study was to evaluate the redox state in the skeletal and cardiac muscles in rats induced to diabetes model and submitted to swimming exercise training for 4 weeks. Methods: Wistar rats were separated into the following four groups: untrained control (C); trained control (T); untrained alloxan-induced diabetes (D); trained alloxan-induced diabetes (TD). The animals were submitted to swimming exercise training for 4 weeks. The redox state of skeletal and cardiac muscles was analyzed by TBARS, -SH groups and, H2O2 production as well as by the SOD and Catalase activity. Besides, a histomorphometry analysis was performed in the cardiac muscle in which the total number of cardiomyocytes and the total area of collagen fibers were measured.Results: On the Soleus muscles, the TD group showed increased of H2O2 and Catalase compared to T group; and SOD compared to D group. About the red gastrocnemius, TD group presented higher SOD and lower Catalase relation to D group. Regarding to cardiac muscle, TD group presented lower TBARS than D group, while it was higher in -SH groups and Catalase compared to D group. Moreover, the swimming exercise led to a decrease in hyperglycemia, benefits in pathogenesis and physio-pathogenesis, evidenced by a reduced number of cardiomyocytes and area of collagen fibers. Conclusions: The swimming protocol exercise in diabetic rats was able to control the hyperglycemia, control of oxidative damage and minimize the fibrosis on cardiac muscle in diabetic rats.
Genome-Wide Occupancy Reveals The Localization of H1T2 (H1fnt) To Repeat Regions and A Subset of Transcriptionally Active Chromatin Domains in Mammalian Spermatids
Authors: Vasantha Shalini , Bhaduri Utsa , Ravikkumar Anjhana C , Rengarajan Anusha , Rao Satyanarayana 
Published: July 2020

Abstract
Background: H1T2/H1FNT is a germ cell specific linker histone variant expressed during spermiogenesis specifically in round and elongating spermatids. Infertile phenotype of homozygous H1T2 mutant male mice revealed the essential function of H1T2 for the DNA condensation and histone to protamine replacement in spermiogenesis. However, the mechanism by which H1T2 imparts the inherent polarity within spermatid nucleus including the additional protein partners and the genomic domains occupied by this linker histone are unknown.Results: Sequence analysis revealed the presence of Walker motif, SR domains and putative coiled-coil domains in the C-terminal domain of rat H1T2 protein. Genome wide occupancy analysis using highly specific antibody against the CTD of H1T2 demonstrated the binding of H1T2 to the LINE L1 repeat elements and to a significant percentage of the genic regions (promoter-TSS, exons and introns) of the rat spermatid genome. Immunoprecipitation followed by mass spectrometry analysis revealed the open chromatin architecture of H1T2 occupied chromatin encompassing the H4 acetylation and other histone PTMs characteristic of transcriptionally active chromatin. In addition, the present study has identified the interacting protein partners of H1T2 associated chromatin mainly as nucleo-skeleton components, RNA binding proteins and chaperones.Conclusions: Linker histone H1T2 possesses unique domain architecture which can account for the specific functions associated with chromatin remodeling events facilitating the initiation of histone to transition proteins/protamine transition in the polar apical spermatid genome. Our results directly establish the unique function of H1T2 in nuclear shaping associated with spermiogenesis by mediating the interaction between chromatin and nucleo-skeleton, positioning the epigenetically specialized chromatin domains involved in transcription coupled histone replacement initiation towards the apical pole of round/elongating spermatids.
Targeting monoamine oxidase A-regulated TAM polarization for cancer immunotherapy
Authors: Wang Yu-Chen , Wang Xi , Yu Jiaji , Ma Feiyang , Li Zhe , Zhou Yang , Zeng Samuel , Ma Xiaoya , Li Yanruide , Neal Adam , Huang Jie , To Angela , Clarke Nicole , Memarzadeh Sanaz , Pellegrini Matteo , Yang Lili 
Published: July 2020

Abstract
Targeting tumour-associated macrophages (TAMs) is a promising strategy to modify the immunosuppressive tumour microenvironment and improve cancer immunotherapy. Monoamine oxidase A (MAO-A) is an enzyme best known for its function in the brain; small molecule MAO inhibitors (MAOIs) are clinically used for treating neurological disorders. Here we observed MAO-A induction in mouse and human TAMs. MAO-A-deficient mice exhibited decreased TAM immunosuppressive functions corresponding with enhanced antitumour immunity. MAOI treatment induced TAM reprogramming and suppressed tumour growth in preclinical mouse syngeneic and human xenograft tumour models. Combining MAOI and anti-PD-1 treatments resulted in synergistic tumour suppression. Clinical data correlation studies associated high intratumoural MAOA expression with poor patient survival in a broad range of cancers. We further demonstrated that MAO-A promotes TAM immunosuppressive polarization via upregulating oxidative stress. Together, these data identify MAO-A as a critical regulator of TAMs and support repurposing MAOIs for TAM reprogramming to improve cancer immunotherapy.
A Case of Long-Term Survival after Splenectomy for Solitary Splenic Metastasis from Gastric Cancer
Authors: Obana Ayato , Komatsu Nobuo , Aiba Kazuma , Nakanishi Shinya , Abe Masakazu , Yamaguchi Toshiyuki , Hayashi Masahiro , Obi Hayato , Koyama Masamichi , Hashimoto Shinichi 
Published: July 2020

Abstract
Background: Very rarely does a splenic solitary metastasis arise from a gastric carcinoma because splenic metastasis is usually seen in association with widespread visceral metastasis. Splenectomy is considered to be a curative treatment; however, long-term prognosis after splenectomy has scarcely been reported. We report a case of a metachronous and solitary metastasis to the spleen from gastric cancer in which the patient achieved 5-year recurrence-free survival after splenectomy.Case Presentation: An 84-year-old man underwent an open total gastrectomy involving D1+ lymph nodes dissection for gastric cancer located in the cardia (pT3N1M0, pStage IIB). Eighteen months later, a 2-cm solitary hypodense lesion was detected in the spleen by computed tomography (CT). Twenty-three months later, the serum carcinoembryonic antigen (CEA) value elevated to 19.9 ng/ml, and abdominal CT revealed an increase in tumor size to 5 cm. Positron-emission tomography (PET)-CT revealed intense 18F-2-deoxy-2-fluoro-glucose (FDG) uptake in the spleen without the involvement of other organs and lymph nodes. We diagnosed him with solitary splenic metastasis from gastric cancer and performed a splenectomy 26 months after the first surgery. Histological examination revealed that the splenic tumor was a moderately differentiated adenocarcinoma, which was very similar to the primary gastric tumor; the lesion was diagnosed as a metastatic tumor from the previous gastric carcinoma. The patient remains healthy to date without recurrence, 5 years after the splenectomy. Conclusion: We experienced a case of a solitary splenic metastasis from gastric cancer in which 5-year recurrence-free survival was achieved after splenectomy. To determine the surgical indication in patients with splenic metastasis, it is important to differentiate between a solitary lesion or multiple metastasis. Especially, occult metastasis should be excluded by means of several months of follow-up with imaging tests and systemic FDG-PET surveys before splenectomy.
The simple surgery to safely extend the anhepatic time to 30 minutes for rat liver transplantation
Authors: Tang Gaofeng , Zhang Jiabin , Zhao Huibo , Wei Sidong , Zhou shaotang , Chen Guoyong 
Published: July 2020

Abstract
Background

Orthotopic rat liver transplantation (OLT) is widely used, it remains complicated although many renovations have been made.
Methods

OLT was performed for tolerance induction, including whole graft OLTs from close male Sprague Dawley (SD) rat to male SD (whole graft group, n = 21) and 30 minute anhepatic time group (AHT group, n = 11), OLT was performed from 50% male Lewis allograft to male Brown Norway (BN) rats to induce tolerance (half graft group, n = 28), Cyclosporine A was injected once daily for 14 days.
Results

For whole graft group, 30-day survival rate was 85.5% (18/21), the reasons of death were gas embolism due to the missed suturing in 2 cases, blood loss in 1 case. For AHT group, 30-day survival rate 72.7% (8/11), the causes of death were respiratory failure in 1 case, unknown in 2 cases. For tolerance group, 30-day survival rate was 64.3% (18/28), the reasons of death were diarrhea in 4 cases, wound dehiscence in 6 cases. There were no differences in survival in 3 groups (p = 0.289).
Conclusion

The anhepatic time can be extended simply through the change of clamping the diaphragm, which facilitates its application in the research.
Nanobarcodes with multidimensional optical information beyond diffraction limit
Authors: Jin Dayong , Wen Shihui , Liu Yongtao , Wang Fan , Lin Gungun , zhou jiajia , Shi Bingyang , Suh Yung Doug 
Published: July 2020

Abstract
Precise design and fabrication of heterogeneous nanostructures will enable nanoscale devices to integrate multiple desirable functionalities. But due to the diffraction limit (~200 nm), the optical uniformity and diversity within the heterogeneous functional nanostructures are hardly controlled and characterized. Here we report a set of nanobarcodes, each optically active section has its unique nonlinear responses to donut illumination patterns, so that one can discern each unit with super resolution. To achieve this, we first realized an approach of highly controlled epitaxial growth and produced a range of one-dimensional heterogeneous structures. Each section along the nanorod structure display tunable upconversion emissions, in four optically orthogonal dimensions, including colour, lifetime, excitation wavelength, and power dependency. Moreover, we demonstrated a 210 nm single nanorod as the smallest polychromatic light source for the on-demand generation of RGB photonic emissions. Remarkably, within a space of 50 nm, only 1/20th of the excitation wavelength, multiple codes can be successfully coded and decoded in 4 optical dimensions. This precision control enables the fabrication of super capacity geometrical barcodes with theoretical coding capacity up to (24-1)4. This work benchmarks our new ability towards the full control of sub-diffraction-limit optical diversities of single heterogeneous nanoparticles.
Analysis of N6-methyladenosine (m6A) methyltransferase reveals METTL14 and ZC3H13 as tumor suppressor genes in breast cancer
Authors: Gong Pengju , Shao Youcheng , Yang Yan , Song Wenjing , He Xin , Zeng Yifan , Huang Sirui , Wei Lei , Zhang Jingwei 
Published: July 2020

Abstract
Background
Recently, an increasing number of studies have revealed that N6-methyladenosine (m6A) functions as a significant post-transcriptional modification which plays a critical role in the occurrence and progression of enriched tumors by regulating mRNA and non-coding RNA biogenesis. However, the biological function of m6A in breast cancer remains largely unclear.
Materials and Methods
In this study, we used a serious of bioinformatic databases and tools to jointly analyze the expression of m6A methylation transferases (METTL13, METTL14, WTAP, RBM15, RBM15B and ZC3H13) and investigate the prognostic value of METTL14 and ZC3H13 in breast cancer. Besides, we analyzed the downstream carcinogenic molecular mechanisms related to METTL14 and ZC3H13, and their relationship with immune infiltration in breast tumor tissues.
Results
The results showed that METTL14 and ZC3H13 were the down-regulated m6A “writers” in breast cancer. Survival outcomes analysis suggested that abnormally low expression of METTL14 and ZC3H13 could predict unfavorable prognosis in four breast cancer subtypes. Moreover, the down-regulation of them was associated with ER-, PR- and basal-like, TNBC patients, as well as tumor progression (increased Scarff, Bloom and Richardson grade status and Nottingham Prognostic Index classification). Co-expression analysis revealed that METTL14 and ZC3H13 had a strong positive correlation with APC, an antagonist of the Wnt signaling pathway, indicating they might cooperate in regulating proliferation, invasion and metastasis of tumor cells. METTL14, ZC3H13 and APC expression levels had significant positive correlation with infiltrating levels of CD4 + T cells, CD8 + T cells, neutrophils, macrophages and Dendritic cells, and negatively with Treg cells in breast cancer.
Conclusions
This study demonstrated that down-regulation of METTL14 and ZC3H13 which act as two tumor suppressor genes was found in breast cancer and predicted poor prognosis. Their abnormal expression promoted breast cancer invasion by affecting pathways related to tumor progression and mediating immunosuppression.
Prevalence of Overweight and Obesity, and Associations with Socio-Demographic Factors in Kuwait
Authors: Oguoma Victor M , Coffee Neil , Alsharrah Saad , Abu-Farha Mohamed , Al-Refaei Faisal H , Al-Mulla Fahd , Daniel Mark 
Published: July 2020

Abstract
Background: Kuwait is amongst countries in the Gulf region with high income economy. According to the World Health Organisation (WHO), one in five adults in the Gulf region is obese. The aim of this study was to estimate the prevalence of both overweight and obesity, and their relationships with socio-demographic factors, in Kuwait.Methods:A population-based survey of diabetes and obesity in Kuwait – part of the Kuwait Diabetes Epidemiology Program – was conducted between 2011-2013, targeting adults aged 18-82 years using the WHO STEPwise approach to non-communicable disease surveillance. Body mass index (BMI) was calculated to classify overweight and obesity, and waist circumference (WC) used to expressed central obesity. Multivariable logistic regression was used to estimate relationships between socio-demographic factors, overweight (25.0-29.9 kg/m2), obesity (≥30.0 kg/m2) or central obesity (WC≥80cm women; WC≥94cm men).Results:Records for gender (57% Men), age, BMI, governorate and nationality existed for 4963 individuals. Mean age and BMI were 43 years and 30kg/m2, respectively. Non-Kuwaiti nationals were more prevalent than Kuwaitis (87% vs 13%). Prevalence rates for overweight, obesity and central obesity were 38% (95%CI: 37-40%), 42% (95%CI: 41-44%) and 75% (95%CI: 73-76%), respectively. The youngest age group (18-29 years) had rates of 33% (95%CI: 28-39%), 25% (95%CI: 20-30%) and 49% (95%CI:43-55%) for overweight, obesity and central obesity, respectively. In covariate-adjusted analyses, the odds of being overweight was 23% greater for men than for women. Conversely, women had a 29% (95%CI: 4-60%) and 6-fold (95%CI: 5-9-fold) greater odds of obesity/central obesity, respectively, than men. Greater educational attainment, physical activity, and non-Kuwaiti status were associated with lower odds of obesity/central obesity. History of smoking, elevated blood pressure, higher income, being married, greater age and female sex related to greater odds of obesity/central obesity.Conclusion:Overweight was greater in men, obesity greater in women. Overweight and obesity prevalence were high in young adults ages 18-29 years, a significant public health concern. Efforts to integrate mandatory physical education to the school curriculum and promoting the creation of recreation spaces/parks to promote physical activities, will play a vital role in the early prevention of overweight/obesity in Kuwait.
Diagnosis and Prognostic Value of SPARC in Gastric Carcinoma: Database Mining for GCTA
Authors: Ying Diqi , Li Ding , Jin Xiao 
Published: July 2020

Abstract
Gastric carcinoma (GC) remains high incidence and mortality both in developed and developing countries. SPARC is extracellular non-structural matrix glycoprotein. Previous studies were closely associated with bone disease. However, the role of SPARC in GC remains largely unclear. In our study, we explored the diagnosis, prognosis and pathway enrichments value of SPARC in GC. Here, with the data from The Cancer Genome Atlas (TCGA), we used receiver operating characteristic (ROC) curve analysis to estimate the diagnosis value of the SPARC expression, Univariate and multivariate analysis to the prognosis, Gene set enrichment analysis (GSEA) to the signal pathway enrichments. As a result, SPARC expression was significantly higher in the GC tissue samples. Those with high SPARC expression of GC patients were worse prognosis. GSEA shows the gene sets related signal pathways including transforming growth factor (TGF) beta signaling pathway, pathways in cancer, Wnt signaling pathway, Mitogen-activated protein kinase (MAPK) signaling pathway etc. In brief, those results suggest that SPARC can serve as a potential biomarker for GC in diagnosis and prognosis.