bluuebunny/crossref_metadata_2025_split huggingface dataset:
Interactions between the N- and C-termini of the mechanosensitive ion channel AtMSL10 are consistent with a three-step mechanism for activation
Authors: Basu Debarati , Shoots Jennette M , Haswell Elizabeth S 
Published: April 2020

Abstract
Although a growing number of mechanosensitive ion channels are being identified in plant systems, the molecular mechanisms by which they function are still under investigation. Overexpression of the mechanosensitive ion channel MSL (MscS-Like)10 fused to green fluorescent protein (GFP) triggers a number of developmental and cellular phenotypes including the induction of cell death, and this function is influenced by seven phosphorylation sites in its soluble N-terminus. Here, we show that these and other phenotypes required neither overexpression nor a tag, and could also be induced by a previously identified point mutation in the soluble C-terminus (S640L). The promotion of cell death and hyperaccumulation of H2O2 in 35S:MSL10S640L-GFP overexpression lines was suppressed by N-terminal phosphomimetic substitutions, and the soluble N- and C-terminal domains of MSL10 physically interacted. We propose a three-step model by which tension-induced conformational changes in the C-terminus could be transmitted to the N-terminus, leading to its dephosphorylation and the induction of adaptive responses. Taken together, this work expands our understanding of the molecular mechanisms of mechanotransduction in plants.
Cosmology dependence of galaxy cluster scaling relations
Authors: Singh Priyanka , Saro Alex , Costanzi Matteo , Dolag Klaus 
Published: April 2020

ABSTRACT
The abundance of galaxy clusters as a function of mass and redshift is a well known powerful cosmological probe, which relies on underlying modelling assumptions on the mass-observable relations (MOR). Some of the MOR parameters can be constrained directly from multi-wavelength observations, as the normalization at some reference cosmology, the mass-slope, the redshift evolution, and the intrinsic scatter. However, the cosmology dependence of MORs cannot be tested with multi-wavelength observations alone. We use magneticum simulations to explore the cosmology dependence of galaxy cluster scaling relations. We run fifteen hydrodynamical cosmological simulations varying Ωm, Ωb, h0, and σ8 (around a reference cosmological model). The MORs considered are gas mass, baryonic mass, gas temperature, Y and velocity dispersion as a function of virial mass. We verify that the mass and redshift slopes and the intrinsic scatter of the MORs are nearly independent of cosmology with variations significantly smaller than current observational uncertainties. We show that the gas mass and baryonic mass sensitively depends only on the baryon fraction, velocity dispersion, and gas temperature on h0, and Y on both baryon fraction and h0. We investigate the cosmological implications of our MOR parametrization on a mock catalogue created for an idealized eROSITA-like experiment. We show that our parametrization introduces a strong degeneracy between the cosmological parameters and the normalization of the MOR. Finally, the parameter constraints derived at different overdensity (Δ500c), for X-ray bolometric gas luminosity, and for different subgrid physics prescriptions are shown in the appendix.
The mystery of photometric twins DES17X1boj and DES16E2bjy
Authors: Pursiainen M , Gutiérrez C P , Wiseman P , Childress M , Smith M , Frohmaier C , Angus C , Castro Segura N , Kelsey L , Sullivan M , Galbany L , Nugent P , Bassett B A , Brout D , Carollo D , D’Andrea C B , Davis T M , Foley R J , Grayling M , Hinton S R , Inserra C , Kessler R , Lewis G F , Lidman C , Macaulay E , March M , Möller A , Müller T , Scolnic D , Sommer N E , Swann E , Thomas B P , Tucker B E , Vincenzi M , Abbott T M C , Allam S , Annis J , Avila S , Bertin E , Brooks D , Buckley-Geer E , Burke D L , Carnero Rosell A , Carrasco Kind M , da Costa L N , De Vicente J , Desai S , Diehl H T , Doel P , Eifler T F , Everett S , Flaugher B , Frieman J , García-Bellido J , Gaztanaga E , Gerdes D W , Gruen D , Gruendl R A , Gschwend J , Gutierrez G , Hollowood D L , Honscheid K , James D J , Kim A G , Krause E , Kuehn K , Maia M A G , Marshall J L , Menanteau F , Miquel R , Ogando R L C , Palmese A , Paz-Chinchón F , Plazas A A , Roodman A , Sanchez E , Scarpine V , Schubnell M , Serrano S , Sevilla-Noarbe I , Suchyta E , Swanson M E C , Tarle G , Wester W 
Published: April 2020

ABSTRACT
We present an analysis of DES17X1boj and DES16E2bjy, two peculiar transients discovered by the Dark Energy Survey (DES). They exhibit nearly identical double-peaked light curves that reach very different maximum luminosities (Mr = −15.4 and −17.9, respectively). The light-curve evolution of these events is highly atypical and has not been reported before. The transients are found in different host environments: DES17X1boj was found near the nucleus of a spiral galaxy, while DES16E2bjy is located in the outskirts of a passive red galaxy. Early photometric data are well fitted with a blackbody and the resulting moderate photospheric expansion velocities (1800  km s−1 for DES17X1boj and 4800  km s−1 for DES16E2bjy) suggest an explosive or eruptive origin. Additionally, a feature identified as high-velocity Ca ii absorption ($v$ ≈ 9400 km s−1) in the near-peak spectrum of DES17X1boj may imply that it is a supernova. While similar light-curve evolution suggests a similar physical origin for these two transients, we are not able to identify or characterize the progenitors.
Clinical impact of urinary CD11b and CD163 on the renal outcomes of anti-neutrophil cytoplasmic antibody-associated glomerulonephritis
Authors: Yokoe Yuki , Tsuboi Naotake , Imaizumi Takahiro , Kitagawa Akimitsu , Karasawa Munetoshi , Ozeki Takaya , Endo Nobuhide , Sawa Yuriko , Kato Sawako , Katsuno Takayuki , Maruyama Shoichi , Yamagata Kunihiro , Usui Joichi , Nagata Michio , Sada Ken-Ei , Sugiyama Hitoshi , Amano Koichi , Arimura Yoshihiro , Atsumi Tatsuya , Yuzawa Yukio , Dobashi Hiroaki , Takasaki Yoshinari , Harigai Masayoshi , Hasegawa Hitoshi , Makino Hirofumi , Matsuo Seiichi ,   Japan Research Committee of the Ministry of Health, Labour, and Welfare for Intractable Vasculitis and for Intractable Renal Disease
Published: April 2020

Abstract

Background
The detection of leukocyte-derived CD11b (α subunit of integrin Mac-1) and CD163 (scavenger receptor) in urine may reflect renal inflammation in antineutrophil cytoplasmic antibody-associated glomerulonephritis (ANCA-GN). The objective of this study was to evaluate the clinical significance of urinary CD11b (U-CD11b) and CD163 (U-CD163) in ANCA-GN.

Methods
U-CD11b and U-CD163 were examined using enzyme-linked immunosorbent assay in ANCA-GN urine samples from our institutional cohort (n = 88) and a nationwide cohort (n = 138), and their association with renal histology was subsequently analyzed. Logistic regression analyses were performed on a nationwide ANCA cohort to determine the associations of the two urinary molecules with renal remission failure at 6 months or with yearly estimated glomerular filtration rate (eGFR) slope over a 24-month observation period.

Results
U-CD11b and U-CD163 were significantly associated with cellular crescent formation and leukocyte accumulation in glomerular crescents. With regard to interstitial inflammation, both levels of U-CD11b and U-CD163 at diagnosis remarkably increased in ANCA-GN compared with the levels observed in nonglomerular kidney disorders including nephrosclerosis, immunoglobulin G4-related disease and tubulointerstitial nephritis; however, the presence of U-CD11b alone was significantly correlated with tubulointerstitial leukocyte infiltrates. Although neither U-CD11b nor U-CD163 at diagnosis was associated with remission failure at 6 months, multivariate analysis demonstrated that the baseline U-CD11b levels were significantly associated with the increase in eGFR following immunosuppressive therapy.

Conclusions
Although both U-CD11b and U-CD163 reflect renal leukocyte accumulation, U-CD11b at diagnosis provides additional clinical value by predicting the recovery rate after the treatment of ANCA-GN.
The effect of fish oil supplementation on the promotion and preservation of lean body mass, strength, and recovery from physiological stress in young, healthy adults: a systematic review
Authors: Heileson Jeffery L , Funderburk LesLee K 
Published: April 2020

Abstract

Context
Military personnel are subjected to physiologically stressful environments during combat and its associated training. Evidence suggests that fish oil–derived n-3 polyunsaturated fatty acids (FO n-3 PUFAs) may affect military personnel’s performance by promoting or preserving lean body mass, strength, and power, while enhancing recovery from training-associated muscle damage.

Objective
Following PRISMA guidelines, this systematic review assessed the evidence for FO n-3 PUFA supplementation across various military-relevant outcomes related to physical performance in healthy adult populations.

Data Sources
The PubMed, Embase, and the CINAHL databases were searched along with references lists of selected articles and reviews.

Data Extraction
Eighteen trials were assessed for bias, and descriptive data were extracted.

Data Analysis
Of the 18 studies included, 12 trials favored FO n-3 PUFA supplementation in ≥ 1 of the performance outcomes.

Conclusion
Overall, FO n-3 PUFA supplementation likely preserves strength and very likely enhances recovery from physiological stress in young, healthy adults. However, FO n-3 PUFAs’ role in promoting or preserving lean body mass or promoting strength is unclear and warrants additional investigation.

Systematic Review Registration
PROSPERO registration no. CRD42020152786.
How do expectant fathers respond to infant cry? Examining brain and behavioral responses and the moderating role of testosterone
Authors: Khoddam Hannah , Goldenberg Diane , Stoycos Sarah A , Horton Katelyn Taline , Marshall Narcis , Cárdenas Sofia I , Kaplan Jonas , Saxbe Darby 
Published: April 2020

Abstract
Expectant parents’ responses to infant cry may indicate future risk and resiliency in the parent-child relationship. Most studies of parental reactivity to infant cry have focused on mothers, and few studies have focused on expectant fathers, although fathers make important contributions to parenting. Additionally, although different responses to infant cry (behavioral, psychological and neural) are hypothesized to track together, few studies have analyzed them concurrently. The current investigation aimed to address these gaps by characterizing multimodal responses to infant cry within expectant fathers and testing whether prenatal testosterone moderates these responses. Expectant fathers responded to infant cry vs frequency-matched white noise with increased activation in bilateral areas of the temporal lobe involved in processing speech sounds and social and emotional stimuli. Handgrip force, which has been used to measure parents’ reactivity to cry sounds in previous studies, did not differentiate cry from white noise within this sample. Expectant fathers with higher prenatal testosterone showed greater activation in the supramarginal gyrus, left occipital lobe and precuneus cortex to cry sounds. Expectant fathers appear to interpret and process infant cry as a meaningful speech sound and social cue, and testosterone may play a role in expectant fathers’ response to infant cry.
The Gray Area of Defining Sexual Assault: An Exploratory Study of College Students’ Perceptions
Authors: Baldwin-White Adrienne , Bazemore Brooke 
Published: April 2020

Abstract
College-age women are disproportionately at risk of experiencing sexual assault. Sexual assault causes tremendous psychological distress for survivors. Therefore, it is imperative to understand the perceptions that affect the perpetration of sexual assault. One of those potential factors is how college students conceptualize and define sexual assault. The current qualitative study demonstrated that college students do not have consistent images of sexual assault and often rely on their own perceptions and experiences to define it. Creating and providing a standard of sexual assault based on the reality of college students can provide a foundation for creating effective prevention strategies.
Sugammadex versus Neostigmine for Reversal of Neuromuscular Blockade and Postoperative Pulmonary Complications (STRONGER)
Authors: Kheterpal Sachin , Vaughn Michelle T. , Dubovoy Timur Z. , Shah Nirav J. , Bash Lori D. , Colquhoun Douglas A. , Shanks Amy M. , Mathis Michael R. , Soto Roy G. , Bardia Amit , Bartels Karsten , McCormick Patrick J. , Schonberger Robert B. , Saager Leif 
Published: April 2020

Abstract

Background
Five percent of adult patients undergoing noncardiac inpatient surgery experience a major pulmonary complication. The authors hypothesized that the choice of neuromuscular blockade reversal (neostigmine vs. sugammadex) may be associated with a lower incidence of major pulmonary complications.

Methods
Twelve U.S. Multicenter Perioperative Outcomes Group hospitals were included in a multicenter observational matched-cohort study of surgical cases between January 2014 and August 2018. Adult patients undergoing elective inpatient noncardiac surgical procedures with general anesthesia and endotracheal intubation receiving a nondepolarizing neuromuscular blockade agent and reversal were included. Exact matching criteria included institution, sex, age, comorbidities, obesity, surgical procedure type, and neuromuscular blockade agent (rocuronium vs. vecuronium). Other preoperative and intraoperative factors were compared and adjusted in the case of residual imbalance. The composite primary outcome was major postoperative pulmonary complications, defined as pneumonia, respiratory failure, or other pulmonary complications (including pneumonitis; pulmonary congestion; iatrogenic pulmonary embolism, infarction, or pneumothorax). Secondary outcomes focused on the components of pneumonia and respiratory failure.

Results
Of 30,026 patients receiving sugammadex, 22,856 were matched to 22,856 patients receiving neostigmine. Out of 45,712 patients studied, 1,892 (4.1%) were diagnosed with the composite primary outcome (3.5% sugammadex vs. 4.8% neostigmine). A total of 796 (1.7%) patients had pneumonia (1.3% vs. 2.2%), and 582 (1.3%) respiratory failure (0.8% vs. 1.7%). In multivariable analysis, sugammadex administration was associated with a 30% reduced risk of pulmonary complications (adjusted odds ratio, 0.70; 95% CI, 0.63 to 0.77), 47% reduced risk of pneumonia (adjusted odds ratio, 0.53; 95% CI, 0.44 to 0.62), and 55% reduced risk of respiratory failure (adjusted odds ratio, 0.45; 95% CI, 0.37 to 0.56), compared to neostigmine.

Conclusions
Among a generalizable cohort of adult patients undergoing inpatient surgery at U.S. hospitals, the use of sugammadex was associated with a clinically and statistically significant lower incidence of major pulmonary complications.

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Collapse of Global Neuronal States in Caenorhabditis elegans under Isoflurane Anesthesia
Authors: Awal Mehraj R. , Wirak Gregory S. , Gabel Christopher V. , Connor Christopher W. 
Published: April 2020

Background
A comprehensive understanding of how anesthetics facilitate a reversible collapse of system-wide neuronal function requires measurement of neuronal activity with single-cell resolution. Multineuron recording was performed in Caenorhabditis elegans to measure neuronal activity at varying depths of anesthesia. The authors hypothesized that anesthesia is characterized by dyssynchrony between neurons resulting in a collapse of organized system states.

Methods
Using light-sheet microscopy and transgenic expression of the calcium-sensitive fluorophore GCaMP6s, a majority of neurons (n = 120) in the C. elegans head were simultaneously imaged in vivo and neuronal activity was measured. Neural activity and system-wide dynamics were compared in 10 animals, progressively dosed at 0%, 4%, and 8% isoflurane. System-wide neuronal activity was analyzed using principal component analysis.

Results
Unanesthetized animals display distinct global neuronal states that are reflected in a high degree of correlation (R = 0.196 ± 0.070) between neurons and low-frequency, large-amplitude neuronal dynamics. At 4% isoflurane, the average correlation between neurons is significantly diminished (R = 0.026 ± 0.010; P < 0.0001 vs. unanesthetized) and neuron dynamics shift toward higher frequencies but with smaller dynamic range. At 8% isoflurane, interneuronal correlations indicate that neuronal activity remains uncoordinated (R = 0.053 ± 0.029; P < 0.0001 vs. unanesthetized) with high-frequency dynamics that are even further restricted. Principal component analysis of unanesthetized neuronal activity reveals distinct structure corresponding to known behavioral states. At 4% and 8% isoflurane this structure is lost and replaced with randomized dynamics, as quantified by the percentage of total ensemble variance captured by the first three principal components. In unanesthetized worms, this captured variance is high (88.9 ± 5.4%), reflecting a highly organized system, falling significantly at 4% and 8% isoflurane (57.9 ± 11.2%, P < 0.0001 vs. unanesthetized, and 76.0 ± 7.9%, P < 0.001 vs. unanesthetized, respectively) and corresponding to increased randomization and collapse of system-wide organization.

Conclusions
Anesthesia with isoflurane in C. elegans corresponds to high-frequency randomization of individual neuron activity, loss of coordination between neurons, and a collapse of system-wide functional organization.

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Proliferating Onychomatricoma. Clinical, Dermoscopical, and Pathologic Features of Onychomatricoma New Variant Resembling Onycholemmal/Squamous Cell Carcinoma
Authors: Perrin Christophe , Ambrosetti Damien , Balme Brigitte , Thomas Luc 
Published: April 2020

Abstract:Proliferating onychomatricoma is a new challenging variant of onychomatricoma that can clinically and histologically mimic squamous cell carcinoma/onycholemmal carcinoma. This is a retrospective case series study of the clinicopathologic and dermoscopic features of 6 patients with a pathologic diagnosis of proliferating onychomatricoma, which was conducted in the dermatology and dermatopathology departments of 2 university hospitals and a private nail's dermatology consultation. The clinical, histological, and immunohistochemical features and follow-up of 6 patients with proliferating onychomatricoma were analyzed; we compare our finding with 6 cases of conventional onychomatricoma. The female-to-male ratio was 1:1 with involvement of fingers in 4 and toe in 2. Among the symptoms were verrucous lesion simulating squamous cell carcinoma, nail thickening, periungual erythema, and pain; symptom duration ranged from 5 to 8 years. Clinical, dermoscopical en face free-margin view, and nail-clipping histologic findings reveal a nail wall-like pattern with pitting. Intraoperative, noncontact, polarizing, light dermoscopy was available in 1 case and showed the typical signs of onychomatricoma (OM). Histologically, all cases showed a well-differentiated, infiltrative, squamous, proliferative lesion exhibiting a lobulated and cystic pattern of growth in the dermis. Abrupt keratinization reminiscent of trichilemmal keratinization, but corresponding in fact to keratogenous spheres, was noted as well as a dysmaturative epithelial pattern. No atypical cytomorphological changes were found. Proliferating onychomatricoma is a new variant of onychomatricoma, which can be misdiagnosed as squamous cell carcinoma/onycholemmal carcinoma; its proper recognition may minimize morbidity associated with inappropriate treatment. Proliferating OM can be differentiated from conventional OM clinically by a free-edge wall-like pattern and on histology of nail clipping by the relatively small size of the cavities. Dermoscopic and nail clipping attributes as free-edge honeycomb-like cavities associated with conventional OM are well established and permit a diagnosis of OM without an invasive nail biopsy. The free-edge wall-like pattern is a distinct new dermoscopic and nail-clipping pattern that should raise for the others onychogenic neoplasms and prompt the clinician to obtain a biopsy specimen. In addition to proliferating OM, the differential diagnosis includes a micropapilliferum variant of OM, onychocytic matricoma, and onychocytic carcinoma.
Multilevel analysis exploring the relative importance of workplace resources in explaining nurses’ workload perceptions: Are we setting the right focus?
Authors: Oppel Eva-Maria , Mohr David C. 
Published: April 2020

Background
Nurses’ workload has become increasingly recognized as an important determinant of nurse turnover and shortage and has been also associated with poorer quality of care. Despite strong evidence that heavy workloads have negative consequences, we still lack a comprehensive understanding of the workplace characteristics that contribute most to improving nurses’ workload, the relative importance of each in doing so, or indeed the workplace characteristics and other factors that drive nurses’ perceptions of their workload.

Purpose
The aim of this study was to examine workplace resources as antecedents of nurses’ perceptions of their workload and to investigate their relative importance in explaining workload perceptions. We considered workplace resources related to staffing, professional relationships, and technology.

Methodology
The study sample comprised nurse-reported and administrative data from U.S. Veterans Health Administration hospitals between 2014 and 2017. Our multilevel analyses are based on data from 20,330 nurses working in 273 work groups at 123 hospitals. We developed and empirically tested a theoretical model using multilevel mixed-effects linear regression. The relative importance of workplace resources was assessed by dominance analysis.

Results
Staffing levels, relational climate, and information technology were significantly associated with nurses’ workload perceptions. Dominance analysis indicated that relational resources are the most important measure in explaining nurses’ workload perceptions.

Practice Implications
This is the first study to examine the relative importance of workplace resources in explaining nurses’ perceptions of their workload. Our results suggest that much might be gained by investing in interventions to boost relational resources. In turn, these findings could lead to more targeted, effective, and resource efficient interventions to improve nurses’ workload.