bluuebunny/crossref_metadata_2025_split huggingface dataset:
MiR-22-3p suppresses sepsis-induced acute kidney injury by targeting PTEN
Authors: Wang Xudong , Wang Yali , Kong Mingjian , Yang Jianping 
Published: June 2020

Abstract
Background: Septic acute kidney injury is considered as a severe and frequent complication that occurs during sepsis. The present study was performed to understand the role of miR-22-3p and its underlying mechanism in sepsis-induced acute kidney injury.
Methods: Rats were injected with adenovirus carrying miR-22-3p or miR-NC in the caudal vein before cecal ligation. Meanwhile, HK-2 cells were transfected with the above adenovirus following LPS stimulation. We measured the markers of renal injury (blood urea nitrogen (BUN), serum creatinine (SCR)). Histological changes in kidney tissues were examined by hematoxylin and eosin (H&E), Masson staining, periodic acid Schiff staining and TUNEL staining. The levels of IL-1β, IL-6, TNF-α and NO were determined by ELISA assay. Using TargetScan prediction and luciferase reporter assay, we predicted and validated the association between PTEN and miR-22-3p.
Results: Our data showed that miR-22-3p was significantly down-regulated in a rat model of sepsis-induced acute kidney injury, in vivo and LPS-induced sepsis model in HK-2 cells, in vitro. Overexpression of miR-22-3p remarkably suppressed the inflammatory response and apoptosis via down-regulating HMGB1, p-p65, TLR4 and pro-inflammatory factors (IL-1β, IL-6, TNF-α and NO), both in vivo and in vitro. Moreover, PTEN was identified as a target of miR-22-3p. Furthermore, PTEN knockdown augmented, while overexpression reversed the suppressive role of miR-22-3p in LPS-induced inflammatory response.
Conclusions: Our results showed that miR-22-3p induced protective role in sepsis-induced acute kidney injury may rely on the repression of PTEN.
LPS promotes the progression of sepsis by activation of lncRNA HULC/miR-204-5p/TRPM7 network in HUVECs
Authors: Chen Xinghai , Song Debiao 
Published: June 2020

Abstract
Sepsis is a systemic inflammatory response syndrome caused by infection. Lipopolysaccharide (LPS) has been reported to induce inflammatory responses, and long non-coding RNA highly up-regulated in liver cancer (HULC) expression was associated with the progression of sepsis. But the role and underlying mechanism of HULC in LPS-induced sepsis remain unclear. Cell viability and apoptosis were measured by methyl thiazolyl tetrazolium (MTT) and flow cytometry assays, respectively. The levels of apoptosis-related proteins, inflammatory cytokines and transient receptor potential melastatin7 (TRPM7) were detected by western blot. Reactive oxygen species (ROS), superoxide dismutase (SOD) and malondialdehyde (MDA) levels were detected by dichloro-dihydro-fluorescein diacetate (DCFH-DA) method using commercial kit. HULC, microRNA-204-5p (miR-204-5p) and TRPM7 expressions in serum of sepsis patients and human umbilical vein endothelial cells (HUVECs) were examined by quantitative real-time polymerase chain reaction (qRT-PCR). Dual-luciferase reporter and RNA immunoprecipitation (RIP) assays were used to confirm the interaction between HULC and miR-204-5p, miR-204-5p and TRPM7. LPS stimulation restrained cell viability and facilitated apoptosis, inflammatory injury and oxidative stress in HUVECs. HULC and TRPM7 were increased and accompanied with decreased miR-204-5p expression in serum of sepsis patients. A significant negative correlation between miR-204-5p and HULC or TRPM7 was observed, and there was a positive relationship between expressions of HULC and TRPM7. Importantly, LPS inhibited the cell viability and induced apoptosis, inflammatory injury and oxidative stress of HUVECs by up-regulating the expressions of HULC and TRPM7, and down-modulating miR-204-5p expression. Mechanically, HULC positively regulated TRPM7 expression by sponging miR-204-5p in HUVECs. LPS impaired cell viability, and promoted cell apoptosis, inflammatory response and oxidative stress in HUVECs by regulating HULC/miR-204-5p/TRPM7 axis.
Inhibition of RNA-binding protein HuR reduces glomerulosclerosis in experimental nephritis
Authors: Liu Simeng , Huang Zhimin , Tang Anna , Wu Xiaoqing , Aube Jeffrey , Xu Liang , Xing Changying , Huang Yufeng 
Published: June 2020

Abstract
Recent identification of an RNA-binding protein (HuR) that regulates mRNA turnover and translation of numerous transcripts via binding to an ARE in their 3′-UTR involved in inflammation and is abnormally elevated in varied kidney diseases offers a novel target for the treatment of renal inflammation and subsequent fibrosis. Thus, we hypothesized that treatment with a selective inhibition of HuR function with a small molecule, KH-3, would down-regulate HuR-targeted proinflammatory transcripts thereby improving glomerulosclerosis in experimental nephritis, where glomerular cellular HuR is elevated. Three experimental groups included normal and diseased rats treated with or without KH-3. Disease was induced by the monoclonal anti-Thy 1.1 antibody. KH-3 was given via daily intraperitoneal injection from day 1 after disease induction to day 5 at the dose of 50 mg/kg BW/day. At day 6, diseased animals treated with KH-3 showed significant reduction in glomerular HuR levels, proteinuria, podocyte injury determined by ameliorated podocyte loss and podocin expression, glomerular staining for periodic acid-Schiff positive extracellular matrix proteins, fibronectin and collagen IV and mRNA and protein levels of profibrotic markers, compared with untreated disease rats. KH-3 treatment also reduced disease-induced increases in renal TGFβ1 and PAI-1 transcripts. Additionally, a marked increase in renal NF-κB-p65, Nox4, and glomerular macrophage cell infiltration observed in disease control group was largely reversed by KH-3 treatment. These results strongly support our hypothesis that down-regulation of HuR function with KH-3 has therapeutic potential for reversing glomerulosclerosis by reducing abundance of pro-inflammatory transcripts and related inflammation.
Clinical Reasoning for Speech Sound Disorders: Diagnosis and Intervention in Speech-Language Pathologists' Daily Practice
Authors: Diepeveen Sanne , van Haaften Leenke , Terband Hayo , de Swart Bert , Maassen Ben 
Published: June 2020

Purpose
This study aims to give an insight in clinical reasoning (diagnosis and intervention) of speech-language pathologists (SLPs) in the Netherlands for children with speech sound disorder (SSD).

Method
The study featured a mixed-method (qualitative and quantitative) design. Semistructured interviews containing nondirective, open-ended questions were conducted with 33 SLPs, which were analyzed using a constant comparative analysis. Other SLPs (137) filled out a questionnaire on the same topics. Multiple-choice questions were analyzed by descriptive frequencies, while open-ended questions were analyzed thematically.

Results
The results indicate that SLPs use a variety of assessments to diagnose SSD, complemented by observation and, often, case history. In total, 85 different diagnostic labels were reported. The choice of intervention is based on what is appealing to the child and what matches his or her age as well as on the specific diagnosis and severity. Interventions are used for multiple speech disorders, and according to SLPs, parents play a large role in diagnostics and intervention.

Conclusion
These results reveal the need for (a) a clear and consistent terminology of diagnoses in the field of pediatric SSD, (b) a fast and easy-to-administer comprehensive differential diagnostic instrument in combination with an instrument to assess participation in everyday life, and (c) a tool to conduct a case history online.
Examining Useful Spoken Language in a Minimally Verbal Child With Autism Spectrum Disorder: A Descriptive Clinical Single-Case Study
Authors: Biller Maysoon F. , Johnson Cynthia J. 
Published: June 2020

Purpose
This was a companion study to a previous one (Biller & Johnson, 2019). The purpose was to develop a detailed descriptive profile of a minimally verbal child with a unique medical history and autism spectrum disorder (ASD). The present report describes his social-cognitive and speech sound production abilities in relation to his potentially burgeoning spoken language.

Method
This in-depth, descriptive, clinical single-case study focused on a 3-year-old boy who was diagnosed with a chromosomal abnormality and ASD. The size of his spoken vocabulary fell at the upper limit for classifying a child as minimally verbal. His demographic information was obtained, in addition to general information from his mother. Four social-cognitive and three speech sound production abilities were assessed, as well as his overall performance in both domains. The study included a parent interview and two child assessment sessions.

Results
The child exhibited low social-cognitive and speech sound production abilities for his age, with social-cognitive abilities higher than speech sound production abilities. Comparison with the previous study revealed substantial gaps in social cognition and speech sound production between this child and five other minimally verbal children with ASD. His higher abilities in these two domains co-occurred with his larger spoken vocabulary size.

Conclusions
Although the child's social-cognitive abilities were low for his age, with his speech sound production abilities even lower, both domains were perhaps high enough to support spoken vocabulary at the upper limit for minimally verbal children. Indeed, there appeared to be quantitative and qualitative differences between him and other minimally verbal children in the previous study. The possibility was explored that there is a point or threshold along the developmental continua for social cognition and speech sound production that allows for expansion into useful language.
Grundsätze der Falldokumentation in der Homöopathie
Authors: Bleul Gerhard 
Published: June 2020

ZusammenfassungKriterien der Falldokumentation für die gesamte Medizin und speziell für die Homöopathie wurden in mehreren Konsensus-Konferenzen und Arbeitsgruppen konzipiert. Die wichtigsten Entwicklungen der letzten 2 Jahrzehnte sind in diesem Artikel zusammengefasst.
Returning to Play after Prolonged Training Restrictions in Professional
Collision Sports
Authors: Stokes Keith A. , Jones Ben , Bennett Mark , Close Graeme L. , Gill Nicholas , Hull James H. , Kasper Andreas M. , Kemp Simon P.T. , Mellalieu Stephen D. , Peirce Nicholas , Stewart Bob , Wall Benjamin T. , West Stephen W. , Cross Matthew 
Published: June 2020

AbstractThe COVID-19 pandemic in 2020 has resulted in widespread training disruption in many
sports. Some athletes have access to facilities and equipment, while others have
limited or no access, severely limiting their training practices. A primary concern
is that the maintenance of key physical qualities (e. g. strength, power,
high-speed running ability, acceleration, deceleration and change of direction),
game-specific contact skills (e. g. tackling) and decision-making ability,
are challenged, impacting performance and injury risk on resumption of training and
competition. In extended periods of reduced training, without targeted intervention,
changes in body composition and function can be profound. However, there are
strategies that can dramatically mitigate potential losses, including resistance
training to failure with lighter loads, plyometric training, exposure to high-speed
running to ensure appropriate hamstring conditioning, and nutritional intervention.
Athletes may require psychological support given the challenges associated with
isolation and a change in regular training routine. While training restrictions may
result in a decrease in some physical and psychological qualities, athletes can
return in a positive state following an enforced period of rest and recovery. On
return to training, the focus should be on progression of all aspects of training,
taking into account the status of individual athletes.
Artroplastia de ombro: tenotomia do subescapular versus osteotomia do tubérculo menor
Authors: França Flávio Oliveira , Freitas José Márcio Alves , Mariosa Carlos Alberto Menezes , Bastiani Carlos Eduardo , Godinho Glaydson Gomes 
Published: June 2020

Resumo
Objetivo Avaliar e comparar a função do músculo subescapular entre os grupos submetidos a tenotomia do subescapular (Grupo A) e osteotomia do tubérculo menor (Grupo B), em pacientes tratados por artroplastia anatômica total e parcial de ombros apresentando osteoartrose primária.
Métodos Estudo retrospectivo de pacientes portadores de osteoartrose primária glenoumeral tratados cirurgicamente com prótese anatômica total ou parcial de ombro, avaliados por exame clínico, exames de imagens, escala visual analógica de dor e escore funcional de Constant e Murley. Foram avaliados 28 pacientes, totalizando uma amostra de 32 ombros operados. O seguimento mínimo foi de 12 meses (média 47,45 meses).
Resultados Nos pacientes submetidos à tenotomia do tendão subescapular, dez obtiveram resultado ultrassonográfico com ruptura de sua espessura total (56%). A consolidação ocorreu em todos pacientes submetidos a osteotomia do tubérculo menor. Não houve diferença entre os grupos na comparação do teste Lift Off, da força nos testes Belly Press e Bear hug, e no escore de Constant e Murley.
Conclusões Não encontramos diferença entre os grupos A e B na comparação do teste Lift Off, da força nos testes Belly Press e Bear hug, e no escore de Constant e Murley.
Luxação traumática isolada da articulação trapézio-metacárpica - Relato de dois casos clínicos
Authors: Pires Filipa Porto , Monteiro Eurico Lisboa , Oliveira Filipa , Carvalho Pedro Atilano , Teixeira João Pedro , Miranda António 
Published: June 2020

ResumoA luxação traumática isolada da articulação trapézio-metacárpica é uma lesão rara que faz parte de menos de 1% de todas as lesões de mãos.Os autores apresentam dois casos de luxação traumática isolada da articulação trapézio-metacárpica. Um dos casos foi tratado com redução fechada e imobilização com gesso, e o outro foi tratado com redução fechada, fixação com fios Kirschner, e imobilização com gesso.O primeiro paciente teve um bom resultado funcional e não mostrou sinais de instabilidade trapeziometacarpal. O paciente tratado com fios Kirschner apresentou sinais de instabilidade clínica e subluxação radiológica.A luxação isolada da articulação trapeziometacarpal é uma lesão rara que pode causar instabilidade articular que interfere com a funcionalidade normal da mão e pode resultar em mudanças articulares degenerativas.O melhor manejo dessa lesão ainda é controverso, já que ainda faltam evidências na literatura que mostrem a superioridade de um tratamento em relação ao outro.
Analysis of Conization Results in Patients undergoing Hysterectomy for Uterine Adenocarcinoma
Authors: Drumond Denise Gasparetti , Leite Isabel Cristina Gonçalves , Rodrigues Vivian de Oliveira , Pannain Gabriel Duque , Carvalho Miralva Aurora Galvão , Amaral Renata Guimarães Rabelo do 
Published: June 2020

Abstract
Objective To observe if the histopathological result of a conization performed after cervical adenocarcinoma in situ diagnosis is compatible with the histopathological analysis of a subsequent hysterectomy.
Methods The present descriptive and observational research consisted of the analysis of the medical records of 42 patients who were diagnosed with in situ adenocarcinoma postconization. The analysis consisted of whether there was compatibility between the histopathological reports of conization and hysterectomy and if there was an association between adenocarcinoma in situ and another neoplasia (squamous disease). Interpretation of any immunohistochemistry reports obtained was also performed. In addition, clinical and epidemiological data were also analyzed.
Results A total of 42 conizations were performed, 33 (79%) were cold knife conizations and 9 (21%) were loop electrosurgical excision procedures (LEEPs). Of the patients analyzed, 5 (10%) chose not to undergo subsequent hysterectomy to preserve fertility or were < 25 years old. Out of the 37 patients with adenocarcinoma in situ who underwent subsequent hysterectomy, 6 (16%) presented with residual disease. This finding proved incompatible with the finding of the conizations, which had ruled out invasive cancer.
Conclusion The prevalence of adenocarcinoma in situ increased in the past years. There is still a large part of the medical literature that advocates the use of conservative treatment for this disease, even though it is common knowledge that it is a multifocal disease. However, the majority of studies advocate that hysterectomy should remain the preferred treatment for women who have already completed their reproductive purpose.
Osteoartrite do tornozelo
Authors: Godoy-Santos Alexandre Leme , Fonseca Lucas Furtado , de Cesar Netto Cesar , Giordano Vincenzo , Valderrabano Victor , Rammelt Stefan 
Published: June 2020

ResumoA osteoartrite (OA) é caracterizada por uma degradação crônica, progressiva e irreversível da superfície articular, associada a inflamação articular. A principal etiologia da OA do tornozelo é pós-traumática e sua prevalência é maior entre os jovens e obesos. Apesar dos avanços no tratamento das fraturas ao redor do tornozelo, o risco geral de desenvolver OA pós-traumática do tornozelo após 20 anos do trauma é de quase 40%; especialmente nas fraturas bimaleolares de Weber tipo B e C e fraturas envolvendo a borda tibial posterior. Nas fraturas do tálus, essa prevalência se aproxima de 100%, dependendo da gravidade da lesão e do tempo de seguimento. Nesse cenário, é fundamental a compreensão atual das vias de sinalização moleculares envolvidas na senescência e apoptose dos condrócitos. O tratamento da OA do tornozelo é estagiado e guiado pelos sistemas de classificação, condições locais e do paciente. Os principais problemas são a limitada capacidade de regeneração da cartilagem articular, o baixo suprimento de sangue e a escassez de células-tronco progenitoras.A presente atualização resume evidências científicas básicas recentes da OA pós-traumática do tornozelo, com foco principal nas alterações metabólicas da sinóvia, da cartilagem e do líquido sinovial. Epidemiologia, fisiopatologia, implicações clínicas, e opções de tratamento são também discutidas.
Role of Ultrasound in Neurocritical Care
Authors: Bhatt Rashmi , Khanna Puneet 
Published: June 2020

AbstractBedside point of care ultrasound has acquired an extremely significant role in diagnosis and management of neurocritical care, just as it has in other specialties. Easy availability and increasing expertise have allowed the intensivists to use it in a wide array of situations, such as confirming clinical findings as well as for interventional and prognostic purposes. At present, the clinical applications of ultrasonography (USG) in a neurosurgical patient include estimation of elevated intracranial pressure (ICP), assessment of cerebral blood flow (CBF) and velocities, diagnosis of intracranial mass lesion and midline shifts, and examination of pupils, apart from the systemic applications. Transcranial sonography has also found use in the diagnosis of the cerebral circulatory arrest. An increasing number of clinicians are now relying on the use of ultrasound in the neurointensive care unit for neurological as well as non-neurological indications. These uses include the diagnosis of shock, respiratory failure, deep vein thrombosis and performing bedside procedures.
Different Perceptions among Women and Their Physicians Regarding Contraceptive Counseling: Results from the TANCO Survey in Brazil
Authors: Machado Rogério Bonassi , Ushikusa Thaís Emy , Monteiro Ilza Maria Urbano , Guazzelli Cristina Aparecida Falbo , di Bella Zsuzsanna Jarmy , Politano Carlos Alberto , Sakamoto Luís Carlos 
Published: June 2020

Abstract
Objective The optimal use of contraceptive methods requires that women participate in targeted choice of methods that meet their individual needs and expectations. The Thinking About Needs in Contraception (TANCO) study is a quantitative online survey of the views of health professionals and women on aspects of contraceptive counseling and contraceptive use.
Methods Physicians and women attending clinics for contraception were invited to complete online questionnaires. The research explored the knowledge and use of contraceptive methods, satisfaction with the current method and interest in receiving more information on all methods. Aspects related to contraceptive practice among physicians were gathered in parallel. The results obtained in the Brazilian research were compared with those of the European research, which involved 11 countries.
Results There was a high prevalence of contraceptive use and general satisfaction with the current method. A total of 63% of the women were using short-acting contraceptive (SAC) methods, and 9% were using a long-acting reversible contraceptive (LARC). Sixty-six percent of women were interested in receiving more information on all methods; 69% of women said they would consider LARC if they received more comprehensive information about it. Health professionals tend to underestimate the interest of women in receiving information about contraception in general, and more specifically about LARCs.
Conclusion Despite the high levels of use and satisfaction with the current methods, women were interested in receiving more information on all contraceptive methods. Structured contraceptive counseling based on individual needs and expectations may lead to greater knowledge and a greater likelihood of proper contraceptive choice.
Reading Epilepsy—An Interesting Case Report
Authors: Hadi Mohammed S. , Rao Arjuna , Vooturi Sudhindra , Jayalakshmi Sita 
Published: June 2020

AbstractReading epilepsy is a language induced reflex epilepsy with common ictal manifestations of regional myoclonus. The electroencephalogram (EEG) often shows focal, regional, or generalized discharges, rarely there may not be any associated EEG changes. We report case of a 25-year-old male with three episodes of seizures, precipitated while reading a book. Reported semiology was stuttering while reading with jerking of tongue as perceived by the patient followed by generalized tonic–clonic seizures (GTCS). Magnetic resonance imaging (MRI) of brain with 3 Tesla (3T) was normal. During videoelectroencephalogram (vEEG), reading precipitated recurrent stuttering each lasting for 1 to 2 seconds. Corresponding vEEG showed rhythmic 1/second symmetrical generalized spike and wave discharges lasting for 2 to 4 seconds during episodes of stuttering with intervening normal background activity, followed by generalized spike and wave discharges intermixed with artifacts recorded during secondary generalization. Patient was prescribed levetiracetam and responded well and is currently seizure-free for more than 6 years.
1.5 – 1.6 μm semiconductor lasers with an asymmetric periodic optically coupled waveguide
Authors: Bagaeva O.O. , Danilov A.I. , Ivanov A.V. , Kurnosov V.D. , Kurnosov K.V. , Kurnyavko Yu.V. , Ladugin M.A. , Marmalyuk A.A. , Romantsevich V.I. , Ryaboshtan Yu.L. , Simakov V.A. , Svetogorov V.N. , Chernov R.V. 
Published: June 2020

Abstract
High-power 1.5 – 1.6-μm semiconductor lasers with an asymmetric periodic optically coupled waveguide are developed and their current – voltage, light – current, and spectral characteristics are experimentally studied. The characteristics of these lasers are compared with the characteristics of traditional lasers based on double separate-confinement heterostructures. It is shown that the developed lasers have lower divergence and almost the same threshold and power characteristics as conventional lasers with Fabry – Perot resonators. The developed lasers with a cavity length of 1.6 mm and a mesa-stripe contact width of 3 μm mounted in a housing 11 mm in diameter have a power no lower than 200 mW at a pump current not exceeding 700 mA with a divergence of 25 – 35° versus 45° typical for conventional lasers.