bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Mechanism of Prevention and Treatment of Maxing Shigan Decoction on Pulmonary Heart Injury Caused by Novel Coronavirus Pneumonia
Authors: Liu Dongling , Su Jing , Hou Wenqian , Ren Weiyu , Zheng Yijun , Ning Yanmei , Jin Xiaojie , Liu Yongqi 
Published: August 2020

Abstract
Background

To explore the mechanism of Maxing Shigan Decoction in the treatment of myocardial injury and lung injury through network pharmacology and molecular docking technology.
Methods

In Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP), a Bioinformatics Analysis Tool for Molecular mechANism of Traditional Chinese Medicine (BATMAN-TCM) and the Comparative Toxicogenomics Database, National Center for Biotechnology Information database, Online Mendelian Inheritance in Man database, the compounds and targets of each drug in Maxing Shigan Decoction and the targets of “acute lung intervention” and “myocardial injury” diseases were searched. Gene Ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis were performed by the DAVID database. GOLD 5.1 was used for molecular docking.
Results

Maxing Shigan Decoction includes 327 compounds and 2722 targets, including 30 key targets. 2125 items were obtained by GO enrichment analysis, including 2047 items of Biological Process, 28 items of Cell Composition and 50 items of Molecular Function. The KEGG pathway was enriched to obtain 149 pathways. The results of molecular docking showed that Gancaonin H and Licorice Glycoside E were well combined with Angiotensin-Converting Enzyme 2-A (ACE2-A) and Angiotensin-Converting Enzyme 2-B (ACE2-B), respectively, and Licorice was well combined with Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) and Interleukin 6 (IL-6). Supraene is an active compound of Ephedra. It has good binding with ACE2-A, ACE2-B, GM-CSF and IL-6, respectively. (6Z, 10E, 14E, 18E)-2, 6, 10, 15, 19, 23- hexamethyletracosa-2, 6, 10, 14, 18, 22- hexaene are the active compounds of Almond, which are well combined with ACE2-A, ACE2-B, GM-CSF and IL-6.
Conclusion

Maxing Shigan Decoction may act on ACE2, GM-CSF and IL-6 targets through the active compounds Supraene of Ephedra, and Almond (6Z, 10E, 14E, 18E)-2, 6, 10, 15, 19, 23-hexamethyletracosa-2, 6, 10, 14, 18, 22-hexaene. Gancainin H and licorice Glycide E are the active compounds of Licorice, act on A and B sites of ACE2 respectively, and Glycyrin acts on GM-CSF and IL-6 targets, coordinating multiple signal pathways to play anti-inflammatory and antiviral roles to prevent and treat lung and heart injury caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2).
Metabolic Syndrome and Mental Health in Posttraumatic Stress Disorder Patients
Authors: SHAHMIRI Hassan , TAGHVA Arsia , EBRAHIMI Mohammad-Reza , URIMI Seyyed Behnam HASHEMI , RAHNEJAT Amir-Mohsen 
Published: August 2020

Abstract
Background: Posttraumatic stress disorder (PTSD) is an abnormal physiologic and psychological reaction in person with severe traumatic history. In recent studies, the relationship between PTSD and some other disease apparently unrelated to psychological situations such as cardiovascular diseases, diabetes, and metabolic syndrome has been revealed. Thus, the aim of this study was to survey the prevalence of metabolic syndrome and mental health in PTSD patients. Methods: The research design was retrospective cohort study. Subjects were consisted of 142 Iran-Iraq war veterans with PTSD (age: 40-60 years) and the control group was consisted of 153 veterans without PTSD. Data was collected using questionnaires, physical exams and laboratory tests.Results: Prevalence of metabolic syndrome was 45.1%in PTSD group and 17% in control group. In addition blood pressure, triglyceride and fasting blood sugar in PTSD group were significantly higher than control group (p<0.05). Also, PTSD patients had significant high rates of psychiatric disorders.Conclusion: PTSD patients are more prone to metabolic syndrome and psychiatric disorders than control group.
Network Pharmacological Analysis of Huanglian Jiedu Decoction for Anti- Atherosclerosis
Authors: Wu Kerui , Jiang Lu , Huang Lanlin , He Yaxing , Yan Xia , Wang Dawei 
Published: August 2020

Abstract
Objective: We aimed to predict the possible active components,key targets and pathways of Huanglian Jiedu Decoction(HLJDD) for anti-atherosclerosis. Methods: The TCMSP database was searched to obtain the active components and targets of HLJDD, the GeneCards and OMIM databases were searched to obtain related targets of atherosclerosis, and we obtain the intersection targets of them, which are the potential targets of HLJDD for anti-atherosclerosis.Application of Cytoscape 3.6.0 software to build a herbal-active ingredient-potential target regulation network.We perform protein-protein interaction(PPI) network analysis of potential targets through STRING 11.0 database and obtain the key targets,and the results of PPI network of key targets were visualized by Cytoscape3.6.0 software. Gene ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of the key targets were performed using STRING11.0 database, and we finally constructed the possible pharmacological network of HLJDD for anti-atherosclerosis .Results: We finally obtained 14 key active ingredients of HLJDD, 65 key targets of anti-atherosclerosis, and 14 key active ingredients corresponded to 52 of these targets. These targets are mainly involved in biological processes such as reaction to organic substance, reaction to chemical stimulation,etc.They mainly involved in biological signaling pathways such as pathways in cancer,IL-17 signaling pathway,etc. Conclusion: HLJDD may act on 52 key targets such as PTGS2, HSP90AA1 and RELA through 14 key active ingredients, and influence the signaling pathways including fluid shear stress and atherosclerosis,PI3K-Akt signaling pathway,IL-17 signaling pathway,AGE-RAGE signaling pathway in diabetic complications,TNF signaling pathway,etc.Thus, it may play an anti-atherosclerosis role by inhibiting inflammatory reaction, oxidative stress and improving hemodynamics,etc.
Long-term Condition Management for Prisoners: exploring prevalence and compliance with QOF monitoring
Authors: Wright Nat , Hankins F , Hearty P , Allott D , Allgar V 
Published: August 2020

Abstract
Background: Long-term conditions (LTCs) are a significant cause of morbidity and mortality and prisoner populations have a disproportionately high prevalence of risk factors for LTCs. The size and mean age of the prison population has increased rapidly in recent years. The UK Quality Outcomes Framework (QOF) is a national standardised framework embedded in community general practice with financial remuneration linked to assessment and ongoing review of key clinical outcomes pertaining to LTCs. However, healthcare in prisons in England is not linked to financial remuneration through the QOF framework and prison clinicians are not mandated to adhere to the framework.Methods: Secondary data analysis of data extracted from the prison primary care record pertaining to patient self-report of LTC, level of confirmation by supporting evidence and compliance with QOF monitoring frameworks. Results: 17% of the sample had at least one LTC, the most common condition being asthma, confirmed in 12% of the sample. Having an LTC was associated with female gender and increasing age. Prevalence rates for the other LTCs were hypertension 3%, epilepsy was 3%, coronary heart disease 2%, diabetes 2% and chronic obstructive pulmonary disease 1%. Just 34% of the eligible sample had had a QOF template completed. Higher rates of completion were associated with younger age and there were also statistically significant inter-prison differences. Conclusion: There is a pressing need to embed standardised QOF monitoring systems within an integrated community/prison commissioning framework supported by connectivity between prison and community primary care records of not just the summary care record but also all activity related to QOF compliance. Amongst prisoner populations, there is high prevalence of risk factors for long-term conditions. This research quantifies the burden of disease and highlights under-developed systems for monitoring. There is an opportunity to enhance connectivity between community and prison GP settings to facilitate seamless monitoring of QOF outcomes.
Durable Control of HIV-1 Using a Staphylococcus aureus Cas9-Expressing Lentivirus Co-Targeting Viral Latency and Host Susceptibility
Authors: Chavez Leonard , Reddy Nishith , Raymond Kyle , Bouzidi Mohamed , Desai Shivani , Dossani Zain , Sperber Hannah , Theiler James , Korber Bette , Pillai Satish 
Published: August 2020

Abstract
CRISPR/Cas9 gene editing has the potential to revolutionize the clinical management of HIV-1 infection, and may eliminate the need for antiretroviral therapy (ART). Current gene therapies attempt to either excise HIV-1 provirus or target HIV-1 entry receptors to prevent infection of new cells. Using a viral dynamic model, we determined that combining these two interventions, in the presence or absence of ART, significantly lowers the gene editing efficacy thresholds required to achieve an HIV-1 cure. To implement this dual-targeting approach, we engineered a single lentiviral vector that simultaneously targets multiple highly-conserved regions of the provirus and the host CXCR4 coreceptor, and developed a novel coculture system enabling real-time monitoring of latent infection, viral reactivation, and infection of new target cells. Simultaneous dual-targeting depleted HIV-1-infected cells with significantly greater potency than vectors targeting either virus or host independently, highlighting its potential as an HIV-1 cure strategy.
Quality of Life and Factors Influencing it: A Study Among People Living Near a Waste Treatment Plant
Authors: Phan Long Thanh , Nguyen Gia Thanh , Nguyen Quynh Anh Dac , Nguyen Hieu Song , Nguyen Tin Trung , Watanabe Toru 
Published: August 2020

Abstract
Background: The amount of waste generated has been increasing over the years, while the ability to dispose of it does not meet the needs at waste treatment plants (WTP), resulting in negative consequences on the natural environment and health of the residents living near these plants; these effects can significantly degrade their quality of life (QoL). Although previous studies on the impact of solid waste and wastewater treatment plants have focused on the health problems suffered by nearby residents, the impact of these plants has scarcely been discussed in the context of mental health, social relationships, and the environment. The objectives of this study are to evaluate the QoL as a comprehensive indicator of the impact the presence of a WTP has on nearby residents, and determine the factors that influence their QoL. Methods: A cross-sectional descriptive study was conducted involving 801 subjects, aged 18 and above, who live near the WTP of Hue city, Thua Thien Hue province, Vietnam. The QoL of the subjects was quantitatively assessed using the World Health Organization's (WHO) QoL assessment scale (WHOQOL-BREF). The general, health, and environmental factors influencing QoL were identified using bivariate and multivariate logistic regression analyses. Results: It was found that only 22.6% of the subjects have a good QoL. In particular, the proportions for good psychological health (6.9%) and environment (13.6%) were low, which may indicate an influence of the WTP. Significant factors that degraded the residents’ QoL are: a lower educational level defined by not graduating from high school (OR=2.78; 1.09−7.06), poor health status (OR=2.50; 95% CI: 1.56−4.01), dissatisfaction with water quality (OR=2.41; 95% CI: 1.10−5.25), and unacceptance of the WTP presence (OR=1.70; 95% CI: 1.11−2.60). Moreover, it was found that subjects living within 2 km of the plant had dermatological syndromes and digestive disorders more frequently than those who lived farther away from the plant. They also reported more complaints about water, air, and soil quality, which were probably due to the operation of the WTP. Conclusions: Burying and disposing of solid waste at the WTP could lead to a degradation of the surrounding water and soil environments, while its collection and transportation is believed to cause odor and dust. The efforts of responsible authorities to strictly supervise and inspect these activities at the WTP are essential, not only to protect the surrounding environment but also to improve the QoL of those who live near these plants.
Presentation of Two New Empirical Equations for Point-Centered Quarter Method for Estimating Trees Density in the Forest
Authors: Haidari Reza Hossein 
Published: August 2020

Abstract
Background and objectives: The point-centered quarter(PCQ) method is one of the distance sampling methods. Previously, researchers such as Cottam and Curtis (1954), Pollard(1971), and Haidari et al. (2008) introduced three equations to the estimation of plant density using the above method. The objective of this research includes developing two new formula for the estimation of tree density with the PCQ method,which is not biased toward the tree distribution pattern. Materials and methods: In this study, 40 circular sample plots (1000 m2 as the real state) and PCQ method were measured via a systematic random sampling design (100 m × 100 m) in the educational research forest at Razi University in Dalahoo Mountain in Kermanshah province. Later, through the mentioned formulas, forest stand density was calculated. Then, the obtained density with different formulas of the PCQ method with the circular plot in the spatial pattern of various types (uniform, random, and cluster) was compared in terms of accuracy and precision.Results: The results showed that two new empirical equations for estimating the density of trees in forest stand were more appropriate than the previous equations in terms of accuracy and precision.Conclusion: The new equations have the following important characteristics: Firstly, they are less dependence on the tree distribution pattern than other equations are, and secondly, the number of trees per area unit can be calculated for each sampling point like the equation used for the sample plot methods. Finally, it is suggested to use the new equations for other forests and also other vegetation, such as the grasslands and scrublands.
Hyper-Activation of Endoplasmic Reticulum Stress PERK/Calcineurin/RyR2 Signaling Pathway is Involved in AGEs-Exacerbated Post- Myocardial Infarction Ventricular Arrhythmias
Authors: Liu Zhongwei , Zhang Yong , Pan Shuo , Qiu Chuan , Jia Hao , Wang Yuan , Zhu Haitao 
Published: August 2020

Abstract
Background: The current study was aimed to investigate the mechanisms of advanced glycation end products (AGEs) in exacerbating post- myocardial infarction (MI) ventricular arrhythmias (VAs). Methods: Correlation between premature ventricular contractions (PVCs) and serum AGEs concentrations was analyzed in a cohort consisted of 101 STEMI patients with culprit vessel of left anterior descending artery (LAD). Established MI rat model were treated with AGEs and/or anti- receptor for AGE (RAGE) antibody. Electrocardiography was used to record VAs. Myocytes were isolated from adjacent area around infracted region. Immunofluorescent stains were used to evaluate association between FKBP12.6 (FK506-bindingprotein 12.6) and ryanodine receptor 2 (RyR2). Calcium sparks were evaluated by confocal microscope. Protein expression and phosphorylation were assessed by Western blotting. A colorimetric method was used to determine the enzymatic activity of calcineurin (CaN). [3H]-ryanodine binding assay was carried out to detect the RyR2 channel activity. Results: In the cohort study, significantly increased amount of (PVCs) were found in STEMI patients with diabetes (P<0.05). Serum AGEs concentration was significantly positively correlated with PVCs amount in STEMI patients (r=0.416, P<0.001). Multivariate analysis showed serum AGEs concentration was independently and positively related to frequent PVCs (adjusted hazard ratio, 1.86; 95% CI, 1.09-3.18, P=0.022). In the animal study, increased glucose regulated protein 78 (GRP78) expression, protein kinase RNA-like ER kinase (PERK) phosphorylation, CaN enzymatic activity, FKBP12.6-RyR2 disassociation, RyR2 channel opening and endoplasmic reticulum (ER) calcium releasing were found in MI animals exposed to AGEs, which were attenuated by anti-RAGE antibody treatment. This RAGE blocking also significantly lowered the VAs amount in MI animals exposed to AGEs. Conclusions: Hyper-activation of ER stress- mediated PERK/CaN/RyR2 signaling participated in AGEs- exacerbated post-MI VAs.
Effects of Ceramide Accumulation in Cardiomyocytes Derived from human-induced Pluripotent Stem Cells on Mitochondrial Function and Mitophagy
Authors: Bekhite Mohamed , González-Delgado Andres , Kretzschma Tom , Müller Tina , Wu Jasmine M.F. , Bekfani Tarek , Franz Marcus , Wartenberg Maria , Gräler Markus , Greber Boris , Schulze Christian 
Published: August 2020

Abstract
BackgroundOversupply of fatty acids (FAs) to cardiomyocytes (CMs) is associated with increased ceramide content and elevated the risk of lipotoxic cardiomyopathy. Here we investigate the role of ceramide accumulation on mitochondrial function and mitophagy in cardiac lipotoxicity using CMs derived from human-induced pluripotent stem cells (h-iPSCs). Methods and resultsMature CMs derived from h-iPSCs exposed to the diabetic-like environment or transfected with plasmids overexpressing serine-palmitoyltransferase long chain base subunit 1 (SPTLC1), a subunit of the serine-palmitoyltransferase (SPT) complex, resulted in increased intracellular ceramide levels. Accumulation of ceramides impaired insulin-dependent phosphorylation of Akt through activating protein phosphatase 2A (PP2A) and disturbed gene and protein levels of key metabolic enzymes including GLUT4, AMPK, PGC-1α, PPARα, CD36, PDK4, and PPARγ compared to controls. Analysis of CMs oxidative metabolism using a Seahorse analyzer showed a significant reduction in ATP synthesis-related O2 consumption, mitochondrial basal respiration and respiratory capacity, indicating an impaired mitochondrial function under diabetic-like conditions or SPTLC1-overexpression. Further, ceramide accumulation increased mitochondrial fission regulators such as dynamin-related protein 1 (DRP1) and mitochondrial fission factor (MFF) as well as auto/mitophagic proteins LC3B and PINK-1 compared to control. Incubation of CMs with the specific SPT inhibitor (myriocin) showed a significant increase in mitochondrial fusion regulators the mitofusin 2 (MFN2) and optic atrophy 1 (OPA1) as well as p-Akt, PGC-1 α, GLUT-4, and ATP production. In addition, a significant decrease in auto/mitophagy and apoptosis was found in CMs treated with myriocin.ConclusionsOur results suggest that ceramide accumulation has important implications in driving insulin resistance, oxidative stress, increased auto/mitophagy, and mitochondrial dysfunction in the setting of lipotoxic cardiomyopathy. Therefore, modulation of the de novo ceramide synthesis pathway may serve as a novel therapeutic target to treat metabolic cardiomyopathy.
Girl-Only Clubs’ Influence on SRH Knowledge, HIV Risk Reduction, and Negative SRH Outcomes among Very Young Adolescent Girls in Rural Malawi
Authors: Manda Wanangwa Chimwaza , Pilgrim Nanlesta , Kamndaya Mphatso , Mathur Sanyukta , Sikweyiya Yandisa 
Published: August 2020

Abstract
BackgroundEarly adolescence is an important period to lay the foundation for positive sexual health development that can overcome sexual and reproductive health challenges faced by very young adolescents (VYAs) as they reach puberty and sexual debut. We report on first-hand accounts of experiences of VYA girls who participated in girl-only clubs, and explore the club participation influence on their SRH knowledge to reduce the risk for HIV and negative sexual health outcomes. MethodsThis was a qualitative study in which twenty-three in-depth interviews were conducted with VYA girls aged 12-14 years. These girls were enrolled in girl-only clubs in two rural southern districts in Malawi. The clubs were a part of larger comprehensive HIV prevention project called DREAMS (Determined, Resilient, Empowered, AIDS-free, Mentored, and Safe) which provided an evidence-based core package of interventions to VYAs to prevent HIV. Interventions included improved access to key health services, education support, social skills, asset building, and economic strengthening. Narrative inquiry was used to generate first-hand accounts of the girls’ experiences with club participation. A thematic analysis was used to generate themes from the transcribed stories. ResultsSix main themes were generated: 1) reasons for joining the clubs with desire to learn about SRH issues serving as the main motivation; 2) influence on gender norms and roles whereby participants described a change of gender roles and norms at home; 3) influence on child abuse practices whereby participants reported a decline in child abusive practices at home ;4) influence on life skills and social networks whereby participants described learning about networking; 5) influence of clubs on school attendance whereby out-of-school girls described how economic empowerment of their guardians facilitated their return to school; and 6) influence of clubs on SRH knowledge acquisition and behaviours whereby participants described acquiring knowledge on sexual health issues.Conclusion Girls-only HIV and SRH programs coupled with economic empowerment for their families can be effective in keeping VYA girls in school and improving SRH knowledge and health seeking behavior.
Feasibility of Single-Incision Clipless Laparoscopic Total Colectomy for intractable slow transit constipation
Authors: Kawahara Hidejiro , Omura Nobuo , Akiba Tadashi 
Published: August 2020

Abstract
Background: In 2014, we reported single-incision clipless laparoscopic total colectomy (SCLTC) with ileorectal anastomosis (IRA) as a type of reduced-port surgery for several benign diseases. Patients with intractable slow transit constipation (STC) have undergone SCLTC with IRA since it was introduced in our institution. However, the feasibility and usefulness of the surgical procedure for patients with intractable STC has not been elucidated.Methods: From January 2011 to December 2018, twenty-two patients with intractable STC underwent SCLTC with IRA at Kashiwa Hospital, Jikei University, were retrospectively registered in this study. We compared the first 12 consecutive patients undergoing the double stapling technique (DST) with IRA (DST group) with the last 10 consecutive patients undergoing functional end-to-end anastomosis (FEEA) with IRA (FEEA group).Results: The mean surgical time was 200.2 (150-249) min for the FEEA group and 230.7 (180-266) min for the DST group. A significant difference was identified between the two groups (0.035). There were no significant differences between the groups with respect to the mean age, sex, constipation type, intraoperative blood loss, or postoperative hospital stay. No postoperative complications were encountered in either group.Conclusion: Although SCLTC cannot be easily introduced for intractable STC, SCLTC with IRA using FEEA is feasible and safe.