bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development and Validation of a Novel Five-Gene-Based RNA Binding Protein Associated Prognostic Model for Human Colon Cancer
Authors: Liu Haoling , Bai Qingquan , Lu Zhaoyang , Song Xuan , Liu Yao , Jin Ye , Meng Fanzheng , Guo Hongrui , Pei Tiemin , Liang Shuhang , Song Ruipeng , Liang Yingjian , Wang Jiabei , Yin Dalong , Qu Zhibo , Guo Huaxin , Jiang Hongchi , Yang Haiyan 
Published: June 2020

Abstract
Background: Dysregulation of RNA binding protein (RBP) expression has been reported in various malignant tumors, and it is related to the occurrence and development of cancer. However, the role of RBPs in colon cancer remains unclear. Methods: We downloaded the RNA sequencing data of colon cancer from The Cancer Genome Atlas (TCGA) database, and determined the differently expressed RBPs between normal and cancer tissues. Then, through a series of bioinformatics analysis, we systematically studied the expression and prognostic value of these RBPs.Result: A total of 490 different expression differently expressed RBPs were identified, including 323 up-regulated and 167 down regulated RBPs. Five RBPs (PNLDC1, NSUN6, NOL3, PPARGC1A, LRRFIP2) were identified as prognosis related genes for the construction of prognostic model. Further analysis showed that the overall survival rate (OS) of patients in the high-risk subgroup was worse than that in the low-risk subgroup based on this model. The area under the characteristic curve of time-dependent receiver was 0.691 in TCGA and 0.624 in GEO, which confirmed the prognostic model to be a good one. We also established a nominal map based on the internal validation in 5 RBPs mRNAs and TCGA sequeues, showing a good ability to differentiate colon cancer.Conclusions: We screened RBPs expression differences between colon cancer and adjacent non tumor colon tissues using the TCGA database to identify potential gene biomarkers.Besides,a very effective prediction model was constructed and tested based on the differential expression of RBPs using the TCGA and Gene Expression Omnibus (GEO) database.We also Validated of the relationship between the expression of five RBPs and prognosis
Neurovascular, Muscle, and Skin Changes on [18f]FDG PET/MRI in Foot Complex Regional Pain Syndrome (CRPS)
Authors: Yoon Daehyun , Xu Yingding , Cipriano Peter W. , Alam Israt S. , Aparici Carina Mari , Tawfik Vivianne L. , Curtin Catherine M. , Carroll Ian R. , Biswal Sandip 
Published: June 2020

Abstract
Background: The goal of this study is to demonstrate the feasibility of [18F]fluorodeoxyglucose (FDG) positron emission tomography (PET)/magnetic resonance imaging (MRI) for non-invasive visualization of muscular, neurovascular, and skin changes secondary to complex regional pain syndrome (CRPS). Methods: Seven adult patients with CRPS in the lower extremity and seven healthy adult controls participated in our [18F]FDG PET/MRI study. All participants received whole-body PET/MRI scans one hour after the injection of 10mCi [18F]FDG. Resulting PET/MRI images were reviewed by two radiologists. Metabolic and anatomic abnormalities identified, were grouped into muscular, neurovascular, and skin lesions. The [18F]FDG uptake of each lesion was compared with that of corresponding areas in controls using a Mann-Whitney U-test. Results: On PET images, muscular, neurovascular, and skin abnormalities were found in 5, 4 and 2 patients, respectively. However, on MRI images, no muscular abnormalities were detected. Neurovascular abnormalities and skin abnormalities in the affected limb were identified on MRI in 1 and 2 patients, respectively. The difference in [18F]FDG uptake between the patients and the controls was significant in muscle (p = 0.018) and neurovascular bundle (p = 0.0005).Conclusions: The increased uptake of [18F]FDG in the symptomatic areas likely reflects the increased metabolism due to the inflammatory response causing pain. Therefore, our approach combining metabolic ([18F]FDG PET) and anatomic (MRI) imaging may offer non-invasive monitoring of the distribution and progression of inflammatory changes associated with CRPS. Trial registration: ClinicalTrials.gov, NCT03195270. Registered 19 June 2017 - Retrospectively registered, https://clinicaltrials.gov/ct2/show/NCT03195270
Plasma Radio–Metabolite Analysis of PET Tracers for Dynamic PET Imaging: TLC and Autoradiography
Authors: Li Fiona , Hicks Justin W , Yu Lihai , Desjardin Lise , Morrison Laura , Hadway Jennifer , Lee Ting-Yim 
Published: June 2020

Abstract
Background In molecular imaging with dynamic PET, the binding and dissociation of a targeted tracer is characterized by kinetics modeling which requires the arterial concentration of the tracer to be measured accurately. Metabolism of the radiolabeled parent tracer in the body due to hydrolysis, methylation and other biochemical processes, results in the production and accumulation of different metabolites in blood which can be labeled with the same PET radionuclide as the parent. Since these radio-metabolites cannot be distinguished by PET scanning from the parent tracer, their contribution to the arterial concentration curve has to be removed for the accurate estimation of kinetic parameters from kinetic analysis of dynamic PET. High performance liquid chromatography has been used to separate and measure radio-metabolites in blood plasma, however, the method is labor intensive and remains a challenge to implement for each individual patient. The purpose of this study is to develop an alternate technique based on thin layer chromatography (TLC) and a sensitive commercial autoradiography system (Beaver, Ai4R, Nantes, France) to measure radio-metabolites in blood plasma of two targeted tracers  [18F]FAZA and [18F]FEPPA , for imaging hypoxia and inflammation respectively. Results Radioactivity as low as 17Bq in 2µL of plasma can be detected on the TLC plate using autoradiography. Peaks corresponding to the parent tracer and radio-metabolites could be distinguished in the line profile through each sample (n=8) in the autoradiographic image. Significant inter-subject and intra-subject variability in radio-metabolites production could be observed with both tracers. For [18F]FEPPA, 50% of plasma activity was from radio-metabolites as early as 5 min post injection while for [18F]FAZA, significant metabolites did not appear until 50 min post. Simulation study investigating the effect of radio-metabolite in the estimation of kinetic parameters indicated that 32-400% parameter error can result without radio-metabolites correction.Conclusion TLC coupled with autoradiography is a good alternative to high performance liquid chromatography for radio-metabolite correction. The advantages of requiring only small blood samples (~ 100 L) and of analyzing multiple samples simultaneously, make the method suitable for individual dynamic PET studies.
Facial Alveolar Bone Thickness and Modifying Factors of Anterior Maxillary Teeth: A Systematic Review and Meta-Analysis of Cone-Beam Computed Tomography Studies
Authors: Rojo-Sanchis Julio , Soto-Peñaloza David , Peñarrocha-Oltra David , Peñarrocha-Diago Miguel , Viña-Almunia Jose 
Published: June 2020

Abstract
Background: Understanding the anatomy of the facial alveolar bone (FAB), provides a prognostic tool for estimating the degree of dimensional ridge alterations after tooth extraction. This study aimed to systematically review the FAB thickness of anterior maxillary teeth measured by CBCT scans. A secondary objective was to assess the facial distance from the cementoenamel junction (CEJ) to the bone crest.Methods: An electronic search was made of Medline, Embase, Web of Science, Cochrane Library and Google Scholar up to December 2019. Studies that analyze and quantitatively compare FAB thickness at maxillary teeth by CBCT scans were included. The methodological quality of the included studies was appraised using the ROBINS-I tool from the Cochrane Collaboration. A meta-analysis of single means, subgroup analysis and meta-regression of covariates were conducted.Results: Twenty-nine studies, including 17,321 teeth, were selected. Seventeen studies considered the facial bone crest, and 12 the CEJ as a reference point for their measurements. Mean FAB thickness was ≤ 1 mm in maxillary incisors and canines (0.75-1.05 mm) and 1-2 mm in premolars. Patients over 50 years of age and females exhibited a thinner FAB at some apico-coronal locations of maxillary incisors and canines. Thicker gingival phenotypes were associated with a thicker FAB in maxillary incisors and canines. The geographical setting proved to be an effect modifier that could explain up to 87% of the heterogeneity in FAB thickness, being Asian populations that showed the lowest FAB thickness values. The CEJ-bone crest distance was 2-2.5 mm in all teeth analyzed. Population over 50 years of age exhibited greater CEJ-bone crest distances, and males also showed a trend for greater distance. The prevalence of bone dehiscences at maxillary incisors and canines was 12.3-20.1%; while the prevalence of fenestrations was between 6.4% and 23.8%.Conclusions: Facial alveolar bone is thin, heterogeneous in width along its apico-coronal dimensions, and increases in thickness in maxillary premolars. The prevalence of < 1 mm FAB thickness was 69.9% in central incisors, 64.5% in lateral incisors, 55% in canines and 40.4% at first premolars. The CEJ-bone crest distance presented homogeneous and similar values in all teeth analyzed.
Bacterial Etiology of Sputum from Tuberculosis Suspected Patients and Antibiogram of the Isolates
Authors: Regmi Ramesh Sharma , Khadka Sujan , Sapkota Sanjeep , Adhikari Sanjib , Dhakal Khuma Kumari , Dhakal Bishnu , Lamsal Bandana , Kafle Sarad Chandra 
Published: June 2020

Abstract
Objective The current study aims to explore the bacteriology of sputum of tuberculosis (TB) suspected patients. A cross-sectional study was carried out in the sputum samples of 150 TB suspected patients visiting District Public Health Office, Bharatpur, Nepal. The samples were subjected to cultural, microscopic and biochemical analyses for the identification of the isolates. In addition, antibiotic susceptibility tests were carried out with a special focus on ESBL and MBL production following Clinical and Laboratory Standard Institute guidelines.ResultsBacterial growth was recovered in 47% (71/150) of the TB suspected patients of which 12.66% (19/150) had pulmonary TB infection. Streptococcus spp. (9%) and Pseudomonas aeruginosa (9%) were the most frequently isolated bacteria. Enterobacteriaceae accounted for 35% of the total isolates. Occurrence of bacterial pathogens was more in males (69%) than in females (31%).The incidence of bacterial pathogen was seen associated with the gender of the patients and with the TB infection (p<0.05) but independent with the age of the patients and HIV infection(p>0.05). Tetracycline was effective against Streptococcus spp. whereas gentamicin was effective against Bacillus spp. Imipenem and co-trimoxazole were effective drugs for Gram-negative isolates. Among 83 isolates, 35 were multi-drug resistant, 9 were ESBL producers and 4 were MBL producers.
A mixed-method study to determine the readiness of medical and health sciences students for interprofessional education in a Gulf university
Authors: Sulaiman Nabil , Hasan Hayder , Rishmawy Youssef , Hussein Amal , Saber-Ayad Maha , Alzubaidi Hamzah , Kawas Sausan Al , Guraya Salman 
Published: June 2020

Abstract
Background High‐quality patient care is a complex phenomenon that requires collaboration among healthcare professionals. Research has shown that Interprofessional Education (IPE) carries promise to improve collaborative work and patient care. So far, collaboration among various medical disciplines remains a challenge. Several survey-based studies have reported attitudes about IPE, but very few mixed methods studies, particularly in Arabic-speaking countries, have been conducted to determine medical students’ perspectives and readiness. Methods A two-staged sequential mixed methods study was conducted among medical and health sciences students of University of [---],[country]. The perspectives of students toward IPE and collaborative practice were first gathered by administering a validated instrument; namely Readiness for Interprofessional Learning Scale (RIPLS). This was followed by detailed focused group discussions. Quantitative and qualitative data analysis were performed using SPSS and NVivo, respectively.Results This study cohort included 282 students. All respondents showed readiness to adopt IPE as all statement of RIPLS survey scored high means. Highest mean of 5 was achieved for IPE elements of identifying and resolving patients’ problems and small group work. Three main themes were generated; prior knowledge, need for IPE framework and its implementation. Information workload, lack of clarity and less focused teaching pedagogies of IPE were considered as perceived barriers. Conclusion This study demonstrates substantial agreement of medical and health sciences students towards readiness and perceived effectiveness of IPE. Educators are urged to embed new IPE program into the existing curricular framework that can potentially enhance collaborative learning and improve quality of patient care.
Mesenchymal stem cells-derived exosomal miRNA-28-3p promotes apoptosis of pulmonary endothelial cells in pulmonary embolism
Authors: Mao Hongyu , Liu Lina , Hu Yamin 
Published: June 2020

Abstract
Background

Pulmonary embolism (PE) is a primary clinical manifestation of venous thromboembolism (VTE). It has been demonstrated that pulmonary endothelial cells (PECs) are apoptotic-resistance in PE. In this study, PECs were collected from PE patients and mouse models. Western blot, RT-PCR, flow cytometry, H&E and TUNEL assay, confocal and TEM microscopy, and luciferase reporter assay were performed to determine the effects of miR-28-3p on PECs apoptosis and if exosomes can act as the shuttle to transport miR-28-3p to PECs.
Material and Methods

The results revealed that apoptosis and miR-28-3p were downregulated in PECs of PE. The miR-28-3p mimics and inhibitor enhanced and further inhibited apoptosis in PECs, respectively.
Results

Both miR-28-3p-modified adipose tissue-derived mesenchymal stem cells (AMSCs) and AMSC-derived exosomes upregulated miR-28-3p expression in PECs, leading to elevated apoptosis of PECs. Apoptosis inhibitor 5 (API5) was a direct target gene of miR-28-3p, and the overexpression of API5 in miR-28-3p-modified PECs further suppressed apoptosis.
Conclusions

Furthermore, the administration of miR-28-3p-modified exosomes to PE mouse model promoted apoptosis in PECs. In conclusion, exosomal miR-28-3p could ameliorate PE-associated apoptosis-resistance in PECs through targeting API5 in vitro and in vivo. Therefore, AMSCs-derived exosome is a promising way to deliver functioning miRNA to PECs, providing insight into novel therapy of PE.
The Impact of Dementia and Language on Hospitalizations. A Retrospective Cohort of Long-Term Care Residents
Authors: Riad Karine , Webber Colleen , Batista Ricardo , Reaume Michael , Rhodes Emily , Knight Braden , Prud’homme Denis , Tanuseputro Peter 
Published: June 2020

Abstract
Background: Hospitalizations carry considerable risks for frail, elderly patients; this is especially true for patients with dementia, who are more likely to experience delirium, falls, functional decline, iatrogenic complications, and infections when compared to their peers without dementia. Since up to two thirds of patients in long-term care (LTC) facilities have dementia, there is interest in identifying factors associated with transitions from LTC facilities to hospitals. The purpose of this study was to investigate the association between dementia status and incidence of hospitalization among residents in LTC facilities in Ontario, Canada, and to determine whether this association is modified by linguistic factors.Methods: We used linked administrative databases to establish a prevalent cohort of 81,188 residents in 628 LTC facilities from April 1st 2014 to March 31, 2017. Diagnoses of dementia were identified with a previously validated algorithm; all other patient characteristics were obtained from in-person assessments. Residents’ primary language was coded as English or French; facility language (English or French) was determined using language designation status according to the French Language Services Act. We identified all hospitalizations within 3 months of the first assessment performed after April 1st 2014. We performed multivariate logistic regression analyses to determine the impact of dementia and resident language on the incidence of hospitalization; we also considered interactions between dementia and both resident language and resident-facility language discordance.Results: The odds of hospitalization were 39% lower for residents with dementia compared to residents without dementia (OR 0.61, 95% CI 0.57–0.65). Francophones had lower odds of hospitalization than Anglophones, but this difference was not statistically significant (OR 0.91, 95% CI 0.81 – 1.03). However, Francophones without dementia were significantly less likely to be hospitalized compared to Anglophones without dementia (OR 0.71, 95% CI 0.53 – 0.94). Resident-facility language discordance did not significantly affect hospitalizations. Conclusions: Residents in LTC facilities were generally less likely to be hospitalized if they had dementia, or if their primary language was French and they did not have dementia. These findings could be explained by differences in end-of-life care goals; however, they could also be the result of poor patient-provider communication.
Study on the Technology of Solid Expandable Tubular in Open-Hole Well
Authors: Zhu Xiaohua , Cheng Feilong , Shi Changshuai , Chen Kailin 
Published: June 2020

Abstract
During the production and drilling of open hole wells, due to the uncertainty and complexity of the rock,The naked hole section is prone to accidents such as collapse of the shaft wall and leakage. In order to solve the above problems, this paper proposes the plugging technology of solid expandable tubualr(SET) in open-hole wells. Based on experimental data,this paper established a dynamic three-dimensional model which considers friction and contact. The author puts forward the applicability criterion of open hole and studies the sensitivity parameters of expansion cone. The research results show that when SET specifications are determined, the borehole size of the open hole well has a great influence on the contact pressure, residual stress, driving force and other factors after the SET is formed, and to a certain extent affects the subsequent service. In addition, the structural parameters of the expansion cone also have a great influence on the plastic forming of SET. The results of this paper provide guidelines and theoretical basis for the use of SET technology in open hole wells.
Preparation, Characterization, and in-vitro Cytotoxicity of Nanoliposomes Loaded With Anti-tuberculous Drugs and TGF-β1 Sirna for Improving Spinal Tuberculosis Therapy
Authors: Niu Ning-Kui , Shi Zhi-Yun , Yang Zong-Qiang , Li Yong-Ai , Shi Jian-Dang , Wang Zi-Li , Liu Hai-Tao , Ma Xiao-Min , ding huiqiang 
Published: June 2020

Abstract
Background: Tuberculosis (TB) represents a bacterial infection affecting many individuals each year and potentially leading to death. Overexpression of transforming growth factor (TGF)-β1 has a major immunomodulatory function in human tuberculosisThis work aimed to develop nanoliposomes to facilitate the delivery of antituberculous products to THP-1-derived human macrophages as Mycobacterium host cells, and to evaluate drug efficiencies as well as the effects of a TGF-β1-specific short interfering RNA (siRNA) delivery system employing nanoliposomes.Methods: siTGF-β1 nanoliposomes loaded with the anti-TB drugs HRZ (isoniazid, rifampicin and pyrazinamide) were prepared, and characterized in vitro, determining the size, zeta potential, morphology, drug encapsulation efficiency (EE), cytotoxicity, and gene silencing efficiency of TGF-β1 siRNA.Results: HRZ/siTGF-β1 nanoliposomes appeared as smooth spheres showing size and positive zeta potential of 168.135±0.5444 nm and +4.03±1.32 mV, respectively. Drug EEs were 90%, 88%, and 37% for INH, RIF, and PZA, respectively. Meanwhile, the nanoliposomes were weakly cytotoxic towards human macrophages as assessed by the MTT assay. Nanoliposomal siTGF-β1 could significantly downregulate TGF-β1 in THP-1-derived human macrophages in vitro.Conclusion: These ﬁndings suggested that HRZ-loaded nanoliposomes with siTGF-β1 have the potential for improving spinal tuberculosis chemotherapy via nano-encapsulation of anti-TB drugs.
Clinical Characteristics and Economic Analysis of Intramedullary Nailing Versus Plate and Screws for Open Distal Tibial Fractures: a Retrospective Study
Authors: Xu Yibo , Ren Cheng , Li Zhong , Zhang Kun , Ma Teng , Liu Deyin , Lu Yao , Li Ming , Liu Hongliang , Tao Wang , Duan Ning , Jiao Ning , Lu Daigang , Wang Qian , Xue Hanzhong 
Published: June 2020

Abstract
Background To analyze the clinical characteristic and economic of intramedullary nailing versus locking compression plate for the treatment of open distal tibial fractures.Methods A retrospective analysis was conducted by enrolling patients with open distal tibial fractures who were admitted in Lower Limb Surgery Ward of Traumatic Orthopedic Department，Xi’an Honghui Hospital from January 2016 to January 2019. The collected clinical materials and data included gender, age, injury mechanism, smoke and alcohol, comorbidity disease, Gustilo classification, days before operation, final treatment option, surgical duration, intraoperative bleeding loss, length of stay, complications, Johner-Wruhs criteria, total cost within 1 year after surgery (including hospitalization expenses and postoperative rehabilitation or follow-up expenses). All the patients were divided into two groups based on the final treatment options: Group IMN(intramedullary nailing) and Group LCP(locking compression plate). Cost data for each case within 1 year after surgery were analyzed for the cost-effectiveness ratio (CER)and incremental cost-effectiveness ratio (ICER) of IMN versus LCP.Results 49 consecutive cases were enrolled including 28 cases of Group IMN and 21 cases of Group LCP with the excellent and good rate of 82.1% (23 cases) and 85.7% (18 cases) respectively based on Johner-Wruhs criteria. The surgical duration (p=0.017) and intraoperative bleeding loss(p=0.046) were significantly lower in Group IMN than in Group LCP. Total cost within 1 year after surgery(p=0.048) was also less in Group IMN（126435.90±39093.98 CNY）than in Group LCP（147834.60±56821.12 CNY）. No statistically significant difference was discovered on the excellent and good rate of Johner-Wruhs criteria between the two groups(p＞0.05). The average cost for every 1% of excellent and good rate was 1540.02 CNY in Group IMN and 1725.02 CNY in Group LCP. Each 1% increasing of excellent and good rate cost 5944.08 CNY more in Group LCP compared with Group IMN.Conclusions Both the IMN and LCP could provide a satisfactory outcome for open distal tibial fractures. However the IMN was a more cost-effective management than LCP when the economic analysis was included.