bluuebunny/crossref_metadata_2025_split huggingface dataset:
Enhanced in vitro production of diosgenin in shoot cultures of Dioscorea deltoidea by elicitation and precursor feeding
Authors: Nazir Romaan , Gupta Suphla , Kumar Vijay , Gupta Ajai Prakash , Dwivedi Padmanabh , Dey Abhijit , Malik Tabarak , Pandey Devendra Kumar 
Published: July 2020

Abstract
Background Dioscorea deltoidea (Family: Dioscoreaceae) is a critically endangered medicinal plant widely used in traditional medicine and pharmaceutical industries for the preparation of appropriate drugs. The present study was conducted to investigate the effect of different elicitors such as salicylic acid (SA) and methyl jasmonate (MeJa) on the synthesis of diosgenin production in D . deltoidea shoot cultures. In addition, the effect of different precursors (squalene, β-sitosterol, and cholesterol) was also demonstrated.Results Results showed that precursors significantly influenced diosgenin production as compared to elicitors. Application of SA (200 µM) showed highest diosgenin production (0.912% DW) at 4 h incubation time, whereas MeJA (100 µM) exhibited 0.814% DW of diosgenin content at 8 h of incubation time. Among precursors, β-sitosterol at 200 µM produced maximum diosgenin content (1.006% DW) followed by 100 µM squalene (0.947% DW) harvested after 5 th day of culture. Interestingly, cholesterol showed low diosgenin production, but significant than control cultures. Conclusions The results revealed that exposure to different elicitors and precursors have a promising application in accumulation of diosgenin in D.deltoidea shoot cultures.
Five Years Follow-up After Arthroscopic Rotator Cuff Repair Using Suture Bridge Technique: Evaluation of the Retear Rate and the Impairment of the Shoulder Function
Authors: Sheng Zhang , Huisheng Shi , Xiaohua Liu , Zheng Wang , Yan Li , Nanling Deng , Jin Sun , Jia Ma , Bo Jiang , Lei Zhang 
Published: July 2020

Abstract
Purpose: To evaluate the retear rate after arthroscopic rotator cuff repair using a suture bridge (SB) technique for patients with full thickness rotator cuff tears and whether the non- healing cuff impaired the shoulder function.Methods: From January 2013 to January 2014, 92 patients in our institution underwent arthroscopic double-row suture bridge repair for the treatment of full-thickness rotator cuff tear 55 patients who have completed the 5 years follow-up were enrolled in this study. There were 36 female and 19 male patients, and the average age was 58.6 years (range, 41- 70 years). The tears were classified into small (<1 cm), medium (1-3 cm), large (3-5 cm), and massive (> 5 cm) according to the classification of DeOrio and Cofield. According to the intraoperative measurement, small tears were found in 5 shoulders (9%), medium tears in 19 shoulders (34.5%), large tears in 23 shoulders (41.8%), and massive tears in 8 shoulders (14.5%). The mean follow-up was 71.2 months (range, 66-78 months). Post-operative cuff integrity was evaluated with ultrasound (US) according to the published literature which was comparable to Sugaya MRI classification. Types I-III indicated cuff healing and types IV or V indicated retear. The retear patterns were divided into type 1 (failure at the original repair site) or 2 (failure around the medial row). At baseline and final follow-up, shoulder functional outcomes were measured using validated, shoulder-specific outcome scores, including the Constant-Murley score (CS) and the American Shoulder and Elbow Score (ASES). The difference in abduction muscle strength was also evaluated between the integrity and the retear group.Results: At final follow-up, the rotator cuff was completely healed in 45 of the 55 shoulders. There were recurrent tears in 10 shoulders, and the retear rate was 18.2%. The type 1 re- tear pattern was found in 4 shoulders (40%), and type 2 in 6 shoulders (60%). The Constant and ASES scores improved from 41.00 to 92.00, and from 41.33 to 88.00, respectively. The mean differences in shoulder scaption strength between the healing and the retear group were 1.1±0.82Kg and 3.0±0.88Kg respectively. The difference in muscle strength between the retear and the healing group was statistically significant (p<0.001).Conclusion: Arthroscopic suture bridge repair of full-thickness rotator cuff tears led to excellent improvement in shoulder function, but maintained a high retear rate. Although the retear group had inferior muscle strength, the patients were still satisfied, and shoulder function was improved due to pain relief. Whether such changes in muscle strength impair patients' long-term quality of life and shoulder function remains to be discussed.Level of evidence IV
Bridging the science-to-service gap: Implementation of a supervised HIV self-testing intervention among young adults at a tertiary education institution in Zimbabwe
Authors: Mukora-Mutseyekwa Fadzai , Mundagowa Paddington Tinashe , Kangwende Rugare Abigail , Murapa Tsitsi , Tirivavi Mandla , Mukuwapasi Waraidzo , Uzande Charles , Mutibura Queen , Chadambuka Elizabeth Marian , Machinga Mazvita 
Published: July 2020

Abstract
Background The evidence-based practices of implementation science constitute the integration of study findings into routine practice of public health interventions such as HIV testing services (HTS). While young people are at high risk of HIV infection, a national survey showed that adolescents and youths were less likely to go for an HIV test. This study aimed to develop and implement a systematic and multifaceted HIV self-testing (HIVST) intervention to promote the uptake of HTS among young people.Methods The study was conducted at Africa University, Mutare where desk and literature review was conducted to gather evidence on HIVST. Multiple stakeholder consultation was carried out to understand contextual enablers and barriers that either promote or undermine adoption of the intervention. Strategies for situation analysis and evidence gathering included community engagement meetings, training of implementers and conducting a baseline survey. A consensus group adapted the Consolidated Framework for Implementation Research (CFIR) to understand the realities of context and intervention while the Reach, effectiveness, adoption, implementation, maintenance (RE-AIM) guide was used to analyze the intervention implementation and evaluation survey.Results The baseline enquiry indicated that the CFIR dimensions which positively influences intervention implementation were: network, network and communications, preparation of implementation, availability of resources, planification, implication and formally appointed internal leadership while origin of intervention and self-efficacy had negatively influenced implementation. Evaluating the intervention fidelity using the RE-AIM framework revealed that high performance with regard to reach, effectiveness, adoption and implementation but low maintenance. This study also applied the APEASE criteria (affordability, practicability, effectiveness, acceptability, side effects, and equity) for selecting the final components used in implementation.Conclusion Combining literature search, documents review, stakeholder consultation, together with provider and end-user engagement can potentially improve intervention delivery for closed populations such as Universities. Blending program agents with systematic process of activities correspondingly enhance HTS uptake among young people. The utilization of the CFIR and RE-AIM analytical frameworks in this study, offered flexible and simplified implementation and analysis of the intervention.
Predicting Pathologic Responsive to Neoadjuvant Chemotherapy in Patients with Locally Advanced Breast Cancer by Radiomics of Multiparametric MR
Authors: Lu nannan , Dong jie , Fang xin , Wang lufang , Jia wei , Zhou xiong , Han xinghua 
Published: July 2020

Abstract
Background:Neoadjuvant chemotherapy for breast cancer was short of recognized treatment scheme and prediction factor. Diffusion weighted magnetic imaging can predict treatment response in patients with locally advanced breast cancer undergoing neoadjuvant chemotherapy. Methods:Fifty patients of breast cancer (mean age, 48.7 years) with stage II-III who underwent neoadjuvant chemotherapy and preoperative MRI were retrospectively evaluated between 2016 and 2020. Association between preoperative breast MRI findings or clinicopathological features and effect of neoadjuvant chemotherapy were studied. Risk factors were identified by multivariate logistic regression analysis. Results:Multivariate logistics regression analysis showed that clinical stage (OR, 0.104; 95% CI: 0.021, 0.516; p=0.006) at baseline and standard apparent diffusion coefficient (ADC) change (OR, 9.865; 95% CI: 1.024, 95.021; p=0.048) were predictive factors of neoadjuvant chemotherapy. The percentage of standard ADC value increasing in pCR group was larger than that in non-pCR group at first time point (p<0.05). The AUC of ROC curve was 0.828 (95% CI: 0.681, 0.975; p<0.05). In pCR group, change of standard ADC values was greater compared with that in tumor size at ﬁrst follow-up (p<0.05). It was correlation between change of standard ADC values and tumor diameter at first follow-up (r, 0.438; p<0.05). Triple-negative patients in pCR group had higher change of standard ADC values than that in non-pCR groupat ﬁrst follow-up (p>0.05).Conclusion:Standard ADC values change, clinical stage at baseline were closely related to the effect of neoadjuvant chemotherapy. The change of standard ADC values advanced reduction of tumor size at first follow-up and can predict effect of neoadjuvant chemotherapy in the early stage.
Dynamic predict in-hospital mortality risk in intensive care unit with a new deep learning of artificial intelligence
Authors: Chen Yu-wen , Li Yu-jie , Yang Zhi-yong , Zhong Kun-hua , Zhang Li-ge , Chen Yang , Zhi Hong-yu , Deng Peng , Wang Dan-dan , Gu Jian-teng , Ning Jiao-lin , Lu Kai-zhi , Zhang Ju , Xia Zheng-yuan , Yi Bin 
Published: July 2020

Abstract
Background

Dynamic prediction of patients’ mortality risk in ICU with time series data is limited due to the high dimensionality, uncertainty with sampling intervals, and other issues. New deep learning method, temporal convolution network (TCN), makes it possible to deal with complex clinical time series data in ICU. We aimed to develop and validate it to predict mortality risk using time series data from MIMIC III dataset.
Methods

Finally, 21139 records of ICU stays were analyzed and in total 17 physiological variables from the MIMIC III dataset were used to predict mortality risk. Then we compared the model performances of attention-based TCN with traditional artificial intelligence (AI) method.
Results

The Area Under Receiver Operating Characteristic (AUCROC) and Area Under Precision-Recall curve (AUC-PR) of attention-based TCN for predicting the mortality risk 48 h after ICU admission were 0.837(0.824–0.850) and 0.454. The sensitivity and specificity of attention-based TCN were 67.1% and 82.6%, compared to the traditional AI method yield low sensitivity (< 50%).
Conclusions

Attention-based TCN model achieved better performance in prediction of mortality risk with time series data than traditional AI methods and conventional score-based models. Attention-based TCN mortality risk model has the potential for helping decision-making in critical patients.
Identification of AC003986.3 as an Independent Risk Factor for Glioma Patient Survival and Functional Analysis
Authors: Liu Qinglin , Ge Huijian , Jiang Peng 
Published: July 2020

Abstract
Background: To validate the potential of AC003986.3 in predicting glioma patient survival and analyze its underlying mechanism.Methods: Gene expression and clinical features of the patients with gliomas were obtained from The Cancer Genome Atlatls. Correlation between AC003986.3 expression profile and patient clinical features and survival were analyzed. Multivariate Cox regression was employed to determine the risk factors for patient survival and construct the prediction model for survival. Validation of the multivariate Cox regression model was performed by comparing the survival curves between the model-predicted high and low death risk subgroups and calculating the accuracy of predicting 1, 2, 3, and 5 years survival by the model. Target genes were predicted with Ensemble Browser. Gene set enrichment analysis was performed to explore AC003986.3 related gene sets enrichment in Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathways.Results: 655 samples with gene expression and complete clinical features were obtained from The Cancer Genome Atlas. Clinical features enrolled in this study were follow up time, survival status, race, gender, race and pathological grade. AC003986.3 expression was positively related to patient age and pathological grade. Patients with High AC003986.3 expression suffered a poorer survival than those with low expression. Multivariate Cox regression revealed that AC003986.3 expression was an independent risk factor for patient survival irrespective of age and pathological grade. Predicted by Ensemble Browser, TWIST1 was identified as the target of AC003986.3. Gene set enrichment analysis revealed that AC003986.3 related gene sets were mainly enriched in cell metabolism.Conclusions: AC003986.3 expression was closely related to age and pathological grade in glioma patients, and was an independent risk factor for patient survival irrespective of age and pathological grade. AC003986.3 was mainly involved in regulating tumor cell metabolism, and this effect is probably mediated by TWIST1.
The role of post-translational modifications by ubiquitin/ISG15 tags in exposed proteins during NETosis: Immunogenic or tolerogenic signaling process in Systemic Lupus Erythematosus?
Authors: Carrillo-Vázquez Daniel Alberto , Jardón-Valadez. Eduardo , Torres-Ruiz Jiram , Juárez-Vega Guillermo , Maravillas-Montero José Luis , Meza-Sánchez David Eduardo , Domínguez-López María Lilia , Varela Jorge Carlos Alcocer , Gomez-Martin Diana 
Published: July 2020

Abstract
Background: Neutrophil extracellular traps (NETs) from patients with Systemic Lupus Erythematosus (SLE) are characterized by lower ubiquitylation and myeloperoxidase (MPO) as a substrate. The structural and functional effect of such modification and if there are additional post-translational modifications (PTMs) are unknown.Methods: To assess the expression and functional role of PTMs in NETs of patients with SLE; reactivation, proliferation and cytokine production was evaluated by flow cytometry using co-cultures with dendritic cells (DC) and CD4+ from SLE patients and healthy controls. The impact of ubiquitylation on MPO was assessed by molecular dynamics. The expression of ISG15 in NETs was evaluated by immunofluorescence and Western Blot.Results: Fifteen patients with SLE and 10 healthy controls were included. In the co-cultures of CD4+ lymphocytes with DC stimulated with ubiquitylated MPO or recombinant MPO, a higher expression of IFNγ and IL17A was found in CD4+ from SLE patients (p < 0.05). Furthermore, with DC stimulated with ubiquitylated MPO a trend towards increased expression of CD25 and Ki67 was found in lupus CD4+ lymphocytes, while the opposite was documented in controls (p < 0.05). Through molecular dynamics we found the K129-K488-K505 residues of MPO as susceptible to ubiquitylation. Ubiquitylation affects the hydration status of the HEME group depending on the residue to which it is conjugated. R239 was found near by the HEME group when the ubiquitin was in K488-K505. In addition, we found greater expression of ISG15 in the SLE NETs vs controls (p < 0.05), colocalization with H2B (r = 0.78) only in SLE samples and increased production of IFNγ in PBMCs stimulated with lupus NETs compared to healthy controls NETs.Conclusion: The ubiquitylated MPO has a differential effect on the induction of reactivation and proliferation of CD4+ lymphocytes in patients with SLE, which may be related to structural changes by ubiquitylation at the catalytic site of MPO. Besides a lower ubiquitylation pattern, NETs of patients with SLE are characterized by the expression of H2B conjugated with ISG15, and the induction of IFNγ by Th1 cells.
Comparison of the RENAL, PADUA, DAP, NePhRO, and SPARE scores for retroperitoneal laparoscopic partial nephrectomy.
Authors: Jin Yanyang , Wang Mingshuai , Yang Feiya , Xing Nianzeng 
Published: July 2020

Abstract
Background: Nephrometry scores play a critical role in the preoperative evaluation of partial nephrectomy. Although score comparisons have been performed for transperitoneal or open surgery, systematic comparisons for retroperitoneal operations are lacking.Methods: We retrospectively evaluated the clinical records of patients who underwent partial nephrectomy at one center by one surgeon. Scores were generated according to the imaging results, and each score was categorized into low-, intermediate- and high-complexity groups. Then, the differences in perioperative outcomes were compared among the groups. We assessed whether the scores and sex, body mass index (BMI), age, or American Society of Anesthesiologists (ASA) Physical Status classification could predict whether the warm ischemia time (WIT) was likely be longer than 20 min and whether they could predict postoperative complications worse than Clavien–Dindo 1. The interobserver variability between two experienced surgeons for these scores was calculated with the intraclass correlation coefficient (ICC).Results: A total of 107 patients were ultimately evaluated. The median tumor size was 36.88 mm, and the median WIT, total operation time (OT) and estimated blood loss (EBL) were 18.97 min, 80 min, and 100 ml, respectively. Significant differences in WIT were identified among the complexity groups for each scoring system. Significant differences were identified between the OTs associated with the DAP and RENAL score complexity groups. The scores included in this study were significantly associated with the probability of having a WIT >20 min and high-grade postoperative complications. Receiver Characteristic Operator (ROC) curves showed that there were no significant differences in their predictive power. NePhRo had the highest agreement (0.839), followed by DAP (0.827). RENAL was superior to SPARE and PADUA, which were 0.758, 0.724 and 0.667, respectively.Conclusions: The scores included in this study were useful for preoperative assessment of retroperitoneal laparoscopic partial nephrectomy. No significant differences were observed among the scores in terms of their ability to predict prolonged hot ischemia time or high-grade postoperative complications. DAP is a good score in the retroperitoneal circumstance when its consistency is taken into account.
Ganoderma (Ganodermataceae, Basidiomycota) species from the Greater Mekong Subregion
Authors: Luangharn Thatsanee , Karunarathna Samantha C , Dutta Arun Kumar , Paloi Soumitra , Lian Cin Khan , Huyen Le Thanh , Pham Hoang ND , Hyde Kevin David , Xu Jianchu , Mortimer Peter E 
Published: July 2020

Abstract

The cosmopolitan Ganoderma is an important pathogen on arboreal plant hosts. Ganoderma is particularly diverse in tropical and temperate regions. It has long been used as traditional medicine because of its beneficial medicinal properties and chemical constituents. In this study, Ganoderma collections were made in tropical regions of Laos, Myanmar, Thailand, Vietnam and temperate Yunnan Province, China. The specimens are described based on micro-macro characteristics and phylogenetic analysis of combined ITS, LSU, TEF1α, and RPB2 sequence data. In this comprehensive study, we report 22 Ganoderma species from temperate and tropical regions of the Greater Mekong Subregion (GMS), namely G. adspersum , G. applanatum , G. australe , G. calidophilum , G. ellipsoideum , G. flexipes , G. gibbosum , G. heohnelianum , G. hochiminhense , G. leucocontextum , G. lingzhi , G. lucidum , G. multiplicatum , G. multipileum , G. myanmarens e , G. orbiforme , G. philippii , G. resinaceum , G. sinense , G. subresinosum , G. williamsianum , and G. tsugae . Of these species, 12 were collected from Yunnan Province, China; three species, were collected from Laos; three species, 2 new records, and one new species were collected from Myanmar; 15 species, and four new records were collected from Thailand; and one new species was collected from Vietnam. Comprehensive descriptions, color photographs of macro and micro characteristics, the distribution of Ganoderma in the GMS and worldwide, and a phylogenetic tree showing the placement of all the Ganoderma reported from the GMS are provided.
Skin-Gut Axis in Psoriasis Setting and Plan for Action: The Effect of Probiotics Supplementation On Clinical Outcomes, Metabolic Endotoxemia, Inflammation, and Cardiovascular Risk in Patients with Psoriasis
Authors: Moludi Jalal , Khedmatgozar Hamed , Tabrizi Fatemeh Pourteymour Fard , Razmi Hamidreza , Amirpour Mehdi , Fathi Pourya 
Published: July 2020

Abstract
Objective: Studies have proposed a link between psoriasis and an imbalanced gut microbiome. Therefore, the modulation of the gut microbiota with probiotics may improve clinical outcomes, metabolic endotoxemia, chronic inflammation, and cardiovascular risk factors in patients with psoriasis.Methods: Forty-six patients with psoriasis randomized into two groups, group 1 received a twice a day capsule having freeze-dried powder with 1.6×109 CFU for eight weeks, and group 2 received placebo. For clinical outcomes, we used the Psoriasis Area and Severity Index (PASI), blood pressure, and SF36 to measure the quality of life. And for biochemistry analysis, we measured pro-inflammatory cytokines (hs-CRP and IL1-β), lipopolysaccharides (LPS), and lipid profile. All the data collected at baseline and the end of the study. Results: Forty-six patients (28 [60%] female; mean [SD] age, 42.51 [14.55] years) contributed to the study. Improvements in SF36 score as an indexed for the QOL was significant in patients taking probiotics supplementation comparing to the placebo group and PASI (-5.06±2.10 vs. 0.30±1.80, P=0.049) as well. After the intervention, the treatment group had significant reductions in LDL and TC compared to the placebo group. In addition, we observed a considerable reduction in serum LPS levels (-7.21±10.33 vs. -2.74±0.97 mmol/L, P=0.010), hs-CRP levels (-1.67 ±0.95 vs. -0.70+ 0.38 mg/L, P=0.013), and IL1- β levels (-1.64 ±1.10 vs. 0.17+ -0.20 mg/L, P=0.043) in the probiotics group. Conclusion: This study shows that probiotics significantly improved the quality of life and seriousness in psoriatic patients. Moreover, it enhances cardiovascular risk factors and inflammatory/oxidative stress markers. Trial registration: This trial also was recorded in the Iranian registry of clinical trials (https://www.irct.ir) (code: IRCT20191124045483N1).
Computational assessment of wind flow field on and around atypical buildings
Authors: KB Rajasekarababu 
Published: July 2020

Abstract
This article provides an overview of pressure coefficients ( Cp ) on atypical tall buildings with the application of CFD. Various modifications in architectural shapes on tall buildings eventually lead to a reduction in the wind load on building surfaces. The surface pressure on conventional (Square and rectangular) buildings is relatively different in comparison to other tall buildings. This study is to evaluate the surface pressure coefficient over rectangular, taper and setback buildings. The computational results show that the taper building has 7% Cp rise at ground level ( y/H= 0.225) in the windward face, and 34% Cp fall at the middle level ( y/H= 0.475) in the side face when compared with the rectangular building. Whereas for the setback building, Cp at ground level near setback ( y/H= 0.225) has reduced to about 25% and about 6% at the middle level ( y/H= 0.475) in windward than that in the rectangle building. Also, the side faces of the setback showed a 15% drop in Cp than other buildings. In leeward face, Cp is reduced to 56% near setback at the top of the building ( y/H= 0.725). This valuation of the Cp on these buildings shows that the effect of setbacks on building reduces the pressure variation on all faces and the downstream wake vortices.