bluuebunny/crossref_metadata_2025_split huggingface dataset:
A case report of greater saphenous vein thrombosis in a patient with coronavirus (COVID-19) infection
Authors: Hesam-Shariati Negin , Fatehi Poya , Fathi Fardin , Abouzaripour Morteza , Shariati Mohamad Bakhtiar Hesam 
Published: October 2020

Abstract
In December 2019, the World Health Organization (WHO) announced a series of pneumonia cases caused by an unknown origin, discovered in Wuhan, China. A dangerous virus called SARS-Cov-2 (severe acute respiratory syndrome coronavirus 2) caused a disease named acute respiratory syndrome, which was later popularly called coronavirus infection (COVID-19). Patients with acute COVID-19 are at high risk for thrombosis in various blood vessels due to over-coagulation, blood stasis, and endothelial damage. To date, very little research has been done on the number and side effects of thromboembolic disorders in patients with COVID-19. In this study, we report a case with COVID-19, who was hospitalized in one of the hospitals in Sanandaj, Iran. There were symptoms of fever, chills, muscle aches, cough, and tachycardia. Laboratory tests such as CRP, ESR, Ferritin CLIA, LDH and D-Dimer were observed in this patient at a high level. Doppler ultrasound of this patient revealed an abnormal finding, thrombosis in the right greater saphenous vein. This suggests that COVID-19 may lead to other side effects through damage to blood vessels.
Drivers of food choices among households in Kersa District, Eastern Ethiopia
Authors: Assefa Nega , Tolera Abebe , Dessie Yadeta , Abraham Aklilu , Demissie Lemma , Teji Kedir , Madzorera Isabel , Canavan Chelsey R , Fawzi Wafaie 
Published: October 2020

Abstract
Introduction: Most individuals in low and middle income countries do not meet nutritional requirements. This can be at least partially attributed to poor diet quality, which is impacted by both external and personal food environment factors. Personal food choice factors among socio-economically disadvantaged groups of Ethiopia are not well researched. Objective: This study aimed to assess food choice factors in eastern Ethiopia. Methods: A cross-sectional quantitative study was conducted among 1196 households in Kersa, Eastern Ethiopia with women age 18-49 years. Confirmatory factor analysis models were used to estimate factors that influence food choice. Cronbach’s alpha for internal reliability coefficients were tested. Results: A majority of respondents were employed in agricultural work and a quarter of the families were poor. Women were primarily responsible for household food procurement. Factors that play a role in food choice included food price, convenience, quality, household preference, household food production and market advertisement. Conclusion: Improving nutritional knowledge and diversifying food availability may help to improve food choices and dietary intake in this rural part of eastern Ethiopia.
Lung segmentectomy assisted by highly selective independent segmental ventilation
Authors: Shi Xiaoshun , Ye Jing , Chen Junyong , Zhai Jianxue , Liu Xiguang , Lu Di , Ni Zhen , Wu Hua , Cai Kaican 
Published: October 2020

Abstract
Background: The identification of targeted intersegmental plane and resection with adequate surgical margin is one of the crucial steps in anatomical pulmonary segmentectomy, and improvements are still needed.Case presentation: Three cases of intersegmental plane identification using highly selective independent segmental ventilation during segmentectomy are reported. All cases required the cooperation with anaesthesiologist who is able to perform segmental ventilation and double confirmation of branch of segmental bronchus by surgeon. The surgical procedure provides a direct visualization of spare segment inflation and saving time in deflation by the conventional residual segment inflation method. Conclusions: Highly selective independent segmental ventilation can be considered a suitable option for the pulmonary intersegmental plane identification, which could be universally used for lung segmentectomy.
Genome-wide detection of CNV regions and their potential association with growth and fatness traits in Duroc pigs
Authors: Qiu Yibin , Ding Rongrong , Zhuang Zhanwei , Wu Jie , Yang Ming , Zhou Shenping , Ye Yong , Geng Qian , Xu Zheng , Huang Sixiu , Cai Gengyuan , Yang Jie , Wu Zhenfang 
Published: October 2020

Abstract
Background: In the process of pig breeding, the average daily gain (ADG), days to 100 kg (AGE), and backfat thickness (BFT) are directly related to growth rate and fatness. However, the genetic mechanisms involved are not well understood. As an essential source of genetic diversity, copy number variation (CNV) that can affect a variety of complex traits and diseases, has gradually been thrust into the limelight. In this study, we reported the genome-wide CNVs of Duroc pigs by SNP genotyping data of 6,627 Duroc pigs. Moreover, we also performed CNV region (CNVR) based association analysis for growth and fatness traits in two Duroc populations.Results: Our study identified a total of 835 nonredundant CNVRs in American and Canadian Duroc pigs, which covered 226.73 Mb (~ 10.00%) of the pig autosomal genome. Among them, we identified 682 CNVRs in the American Duroc pigs and 424 CNVRs in the Canadian Duroc pigs, and 271 CNVRs were detected in both populations. Experimentally, 77.8% of randomly selected CNVRs were validated by quantitative PCR (qPCR). We also identified 24 significant CNVRs for growth and fatness traits though CNVR-based association analysis. Among these, six and three CNVRs in American and Canadian Duroc pigs were found to be associated with both ADG and AGE traits, respectively. Notably, four CNVRs showed both significant in ADG, AGE, and BFT, indicating that these CNVRs may play a pleiotropic role in regulating pig growth and fat deposition. Besides, further bioinformatics analysis determined a subset of potential candidate genes, such as PDGFRB, INSIG1, GPER1, PDGFA, and LPCAT1.Conclusions: The present study provides a necessary supplement to the CNV map of the Duroc genome through a large-scale population genotyping. Also, the CNVR-based association results provide a meaningful way to elucidate the genetic mechanisms of complex traits. The identified CNVRs can be used as molecular markers for genetic improvement in molecular-guided breeding of modern commercial pigs
Down-Regulated gga-miR-223 Inhibits Proliferation and Induces Apoptosis of MG-infected DF-1 Cells by Targeting FOXO3
Authors: Yin Xun , Wang Yingjie , Sun Yingfei , Han Yun , Sun Huanling , Zou Mengyun , Luo Ronglong , Xiuli Peng 
Published: October 2020

Abstract
BackgroundMycoplasma gallisepticum (MG) is a major poultry pathogen that can induce Chronic Respiratory Disease (CRD) in chickens, causing serious economic losses in poultry industry worldwide. Increasing evidence suggests that microRNAs (miRNAs) act as a vital role in resisting microbial pathogenesis and maintaining cellular mechanism. Our previous miRNAs sequencing data showed that gga-miR-223 expression level significantly decreased in MG-infected chicken lungs. The aim of this study was to reveal the role of gga-miR-223 in MG-induced CRD progression. ResultsWe found that gga-miR-223 was remarkably down regulated and forkhead box O3 (FOXO3) was up-regulated in both MG-infected chicken embryos lungs and the chicken embryonic fibroblast cell line (DF-1) by qPCR. FOXO3 was verified as the target gene of gga-miR-223 through bioinformatics analysis and dual-luciferase reporter assay. Further studies showed that overexpressed gga-miR-223 could promote cell proliferation, cell cycle, and inhibit cell apoptosis by notably promoting the expression of cell cycle marker genes cyclin-dependent kinase 1 (CDK1), cyclin-dependent kinase 6 (CDK6) and Cyclin D1 (CCND1) and inhibiting the expression of apoptosis markers Bcl-2-like 11(BIM), FAS ligand (FASLG) and TNF-related apoptosis-inducing ligand (TRAIL). As expected, FOXO3 knockdown group got similar results. Overexpression of gga-miR-223 observably promoted cell multiplication, cell cycle progression, and inhibited apoptosis of MG-infected DF-1 cells, while inhibited gga-miR-223 had the opposite effect. ConclusionsTaken together, upon MG-infection, downregulated gga-miR-223 could decrease proliferation, cycle progression, and increase apoptosis through directly targeting FOXO3 to exert an aggravating MG-infectious effect.
Recurrence of Positive SARS-CoV-2 RNA in a COVID-19 Patient: Two Case Reports from Saudi Arabia
Authors: Alturaif Sarah , Alharbi Remaaz , Alqurashi Waleed , Alsadoon Abdulaziz , Alabdullatif Meshari 
Published: October 2020

Abstract
Background The rapid spread of the COVID-19 pandemic which took a place in Wuhan, China and diffused globally required the international public health to take a coordinated international response. As other coronaviruses were described, SARS-CoV-2 was thought to be an immunizing, monophasic disease. Herein, we reported first two COVID-19 cases registered in Saudi Arabia whose nasopharyngeal swabs turned positive for SARS-CoV-2 ribonucleic acid (RNA) after recovery.Case presentation First patient who is symptomatic with recurrence of positive SARS-CoV-2 RNA occurred 115 days after the first symptomatic infection and did not develop protective immune response. Second patient who is asymptomatic during the recurrence of positive SARS-CoV-2 RNA occurred 60 days after the first symptomatic infection in an apparently immunocompetent patient.Conclusion These findings highlight the recurrence of positive SARS-CoV-2 after recovery even if SARS-CoV-2 antibodies were developed.
Persistent infection with SARS-CoV-2 – first direct evidence
Authors: Liu Tao , Tao Huangheng , Peng Jianping , Zhou Fuling , Liu Tongzu , Wang Xinghuan 
Published: October 2020

Abstract
Whether persistent infections with SARS-CoV-2 exist is still unknown. This study reported a case with COVID-19 who still got positive RT-PCR test results for SARS-CoV-2 in his throat swabs about 4 months post symptom onset. This case is potentially the first direct evidence of persistent infection with SARS-CoV-2.
Implementation, Spread and Impact of The Patient Oriented Discharge Summary (PODS) Across Ontario Hospitals: A Mixed Methods Evaluation
Authors: Hahn-Goldberg Shoshana , Huynh Tai , Chaput Audrey , Krahn Murray , Rac Valeria , Tomlinson George , Matelski John , Abrams Howard , Bell Chaim , Madho Craig , Ferguson Christine , Turcotte Ann , Free Connie , Hogan Sheila , Nicholas Bonnie , Oldershaw Betty , Okrainec Karen 
Published: October 2020

Abstract
BACKGROUND: Traditional discharge processes lack a patient-centred focus. This project studied the implementation and effectiveness of an individualized discharge tool across Ontario hospitals. The Patient Oriented Discharge Summary (PODS) is an individualized discharge tool with guidelines that was co-designed with patients and families to enable a patient-centred process. METHODS: Twenty one acute-care and rehabilitation hospitals in Ontario, Canada engaged in a supported community of practice and worked over a period of 18 months to implement PODS. An effectiveness-implementation hybrid design using a triangulation approach was used with hospital-collected data, patient and provider surveys, and interviews of project teams. Key outcomes included: reach within and spread outside target populations, change in patient-centred processes, patient and provider experience, understanding of discharge instructions, and healthcare utilization. Statistical methods included linear mixed effects models and generalized estimating equations. RESULTS: Of 65,221 discharges across hospitals, 41,884 patients (64%) received a PODS. There was variation in reach and implementation pattern between sites, though none of the between site covariates was significantly associated with implementation success. Both high participation in the community of practice and scoring highly on quality of PODS were associated with higher implementation success. PODS improved family involvement during discharge teaching (7% increase, p=0.026), use of teach-back (11% increase, p<0.001) and discussion of help needed (6% increase, p=0.041). Although unscheduled healthcare utilization decreased with PODS implementation, it was not statistically significant.CONCLUSIONS: This project highlighted the system-wide adaptability and ease of implementing PODS across multiple patient groups and hospital settings. PODS demonstrated an improvement in patient-centred discharge processes linked to quality standards and health outcomes. A community of practice and high quality content may be needed for successful implementation.
Ureteral Calculi Associated with High-Altitude Polycythemia: A Case Report and Literature Review
Authors: Yang Min , Wuren Tanna , Ma Kexiong , Ge Ri-Li , Ji Linhua , Cui Sen 
Published: October 2020

Abstract
Background: High-altitude polycythemia (HAPC) is a common disease in high-altitude areas characterized by excessive erythrocyte proliferation and severe hypoxemia. Recently, the incidence of ureteral calculi has a rising trend. However, cases of ureteral calculi associated with high-altitude polycythemia have not been reported. Case Presentation: We present the cases of two patients with HAPC who were born in the lowlands and worked in high altitudes and were admitted to the hospital with acute severe pain in the ureter as the first symptom. Urological examinations confirmed the presence of a ureteral stone. Interestingly, the biochemical tests showed elevated serum uric acid levels and the calculous component analysis suggested anhydrous uric acid. Recently, extensive research has demonstrated a significant correlation between hyperuricemia and high-altitude polycythemia. Therefore, we speculated that the occurrence of ureteral calculi among immigrants to the plateau might be related to hyperuricemia associated with high-altitude polycythemia. Conclusions: This case report and literature review highlights that the prevention of ureteral calculi in patients with polycythemia who immigrate to the plateaus from high-altitude areas should be considered. Additionally, the serum uric acid levels and urine pH should be monitored regularly.
Use of Lipid Parameters to Identify Healthy Men at a Higher Risk of Arterial Stiffness Progression
Authors: Sang Yu , Cao Ming , Wu Xiaofen , Ruan Lei , Zhang Cuntai 
Published: October 2020

Abstract
Background: Dyslipidemia contributes to the development and progression of arterial stiffness. We aimed to confirm the superiority of the serum lipids and their calculated ratios in predicting arterial stiffness progression.Methods: Total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), and brachial-ankle pulse wave velocity (baPWV) of 612 healthy males (47.0 ± 10.5 years) were measured at baseline. Values for non-HDL-C, TC/HDL-C, TG/HDL-C, LDL-C/HDL-C, and non-HDL-C/HDL-C were calculated. BaPWV was re-performed after 4.1 years follow-up. A baPWV cutoff of 1400 cm/s was used to diagnose arterial stiffness.Results: Over the follow-up period, the mean baPWV value increased from 1330 cm/s to 1400 cm/s, and 309 individuals increased/persisted with high baPWV (outcome 1). Among the 430 subjects who were free of arterial stiffness at baseline, 90 arterial stiffness events occurred (outcome 2). Only logTG (OR 1.65 [95% CI: 1.14-2.40] for outcome 1; 2.08 [1.24-3.52] for outcome 2) and logTG/HDL-C (1.56 [1.15-2.13] for outcome 1; 1.69 [1.10-2.62] for outcome 2) were significantly associated with arterial stiffness progression after adjusting for confounding factors. Adding logTG or logTG/HDL-C to age and blood pressure improved the accuracy of risk predictions for arterial stiffness progression. These associations remained significant when lipids were analyzed as categorical variables.Conclusions: Baseline serum TG and TG/HDL-C were independently associated with increases in/persistently high baPWV and incident arterial stiffness, and they performed more effectively than other lipid variables in identifying healthy men at a higher risk of arterial stiffness progression.
Different Doses of Erenumab for Preventive Treatment of Episodic Migraine: A Systematic Review and Network Meta-Analysis
Authors: Yang Yanbo , Chen Mingjia , Wang Zilan , Sun Yue , Jiang Fan , Chen Zhouqing , Wang Zhong 
Published: October 2020

Abstract
Background Erenumab is a novel monoclonal calcitonin gene–related peptide receptor antibody that is used for the preventive treatment of migraine.Objectives To evaluate overall safety and efficacy and dose-response relationship of erenumab in patients with episodic migraine and patients with prior migraine treatment failures.Methods We searched randomized clinical trials on PUBMED, EMBASE database, and Cochrane Library database. A pair-wise meta-analysis and Bayesian network analysis were performed.Results For efficacy outcomes, the network meta-analysis suggests that compared with erenumab 70 mg, participants received erenumab 140 mg reported significantly decreased MSMD and increased 50% response rate, and erenumab was most likely to be ranked first for MMD, MSMD and 50% response rate. For safety outcomes, the network meta-analysis has found no significant difference between the 70 mg group and the 140 mg group measured by AE and SAE. For patients with ≥2 treatment failures, 140mg erenumab group, patients with ≥2 treatment failures reported significantly reduced MMD and MSMD, increased 50%, and 75% response rate, compared with placebo. For safety outcomes, no significant difference was found between 140 mg erenumab group and the placebo group.Conclusion Erenumab was effective in patients with episodic migraine. 140 mg erenumab was associated with better efficacy outcomes without increased risk for developing adverse events compared with 70 mg erenumab, and 140 mg erenumab was effective in patients with prior migraine treatment failures.Registration number: CRD42020198985
Using Weighted Gene Co-Expression Network Analysis to Identify Key Genes Related to the Onset of Preeclampsia
Authors: Shen Xinyang , Wang Zhijian , Zeng Zhirui , Huang Zhenqin , Huang Xiaowei , Zhang Lizi , Pan Shumin , Xie Lijia , Li Jiaqi , Chen Lu , Yue Xiaojing 
Published: October 2020

Abstract
Background: Preeclampsia is a form of hypertension in pregnancy, which induced by complicated factors. However, the pathogenesis of the disease is unclear. The present study was aimed to discover the critical biomarkers associated with the occurrence and development of preeclampsia. Methods：Gene data profile GSE75010 was downloaded from the Gene Expression Omnibus (GEO) database and used as discovery cohort to establish a WGCNA network determining significant modules which associated with clinical traits. Subsequently, functional enrichment analysis, pathway analysis and protein-protein interaction (PPI) network construction were performed on the core genes in significant modules to identify hub genes. Then, gene data profile GSE25906 was used as verified cohort to determine their diagnostic value of hub genes. The protein expression levels of these hub genes in preeclampsia and control placental tissues were verified using immunohistochemistry method. Finally, GSEA was performed to analyze their enrichment pathways. Results: Total 33 co-expression modules were identified after the establishment of WGCNA, of which 4 gene modules were identified as significant modules because they were related to multiple (>3) clinical traits. Total 75 core genes in significant modules were analyzed, and results showed that they were mainly enriched in adaptive immune response (Gene Ontology term) and platelet activation (Kyoto Encyclopedia of Genes and Genomes term). Finally, a total of 5 genes including TYROBP, PLEK, LCP2, HCK, ITGAM were identified as hub genes which scored high in PPI network and had high diagnostic value. Furthermore, the protein level of these 5 genes in placental tissues of preeclampsia was lower than that of the control group. Moreover, these 5 genes were all enriched in 17 pathways, including autoimmunity pathway. Conclusions：These 5 genes (TYROBP, PLEK, LCP2, HCK, ITGAM) may be closely related to the pathogenesis of preeclampsia, which may also help the diagnosis and therapy of preeclampsia.
A North-South-South Partnership in Higher Education to Develop Health Research Capacity in the Democratic Republic of Congo: the Challenge of Finding a Common Language
Authors: Horwood Christiane , Mapumulo Sphindile , Haskins Lyn , John Vaughn , Luthuli Silondile , Tylleskär Thorkild , Mutombo Paulin , Engebretsen Ingunn M. S. , Ali Mapatano Mala , Hatløy Anne 
Published: October 2020

Abstract
Background: Globally, increasing numbers of higher education institutions (HEIs) in non-English-speaking countries have adopted English as a medium of instruction (EMI), because of the perception that this provides opportunities to attract high calibre students and academic staff, and engage with the international research community. We report an evaluation of a North-South-South collaboration to develop health research capacity in Democratic Republic of Congo (DRC) by establishing a postgraduate programme in nutritional epidemiology at the Kinshasa School of Public Health (KSPH), where English was adopted as the medium of instruction. We report experiences and perceptions of stakeholders, facilitators and students about using EMI.Methods: In-depth qualitative interviews were conducted between October-December 2019 among purposively sampled stakeholders (8), facilitators (11) and students (12) involved in the programme from all three partner institutions (University of Kinshasa; University of KwaZulu-Natal, South-Africa; University of Bergen, Norway). Interviews were conducted in participants’ language of preference (English or French), audio-recorded, transcribed verbatim and translated into English when required. Analysis employed a thematic approach. Results: Most participants viewed EMI positively, reporting that studying in English created opportunities to access relevant literature, improve interactions with the scientific community and advance their careers. As a result of adopting EMI, some students had opportunities to present research findings at international conferences, and publish their research in English. English-speaking researchers from partner institutions were able to participate in supervision of students’ research. However, inadequate English competency, particularly among students, was challenging, with some students reporting being unable to understand or interact in class, which negatively affected their academic performance. Further, EMI created barriers at KSPH among academic staff who were not proficient in English, leading to lack of integration with other postgraduate programmes. Participants suggested additional English language support for EMI.Conclusion: Partnerships between HEIs are a powerful tool to develop research capacity in low income countries and improve research outputs and evidence-based decision-making. EMI was able to develop the common ground required to establish a collaboration between HEIs where several languages were spoken. However, investing in wide-ranging supports to develop English proficiency is essential to ensure that challenges do not outweigh the benefits.