bluuebunny/crossref_metadata_2025_split huggingface dataset:
Rapid growth of large area graphene on glass from olive oil by laser irradiation
Authors: Huang Yihe , Sepioni Margherita , Whitehead David , Liu Zhu , Guo Wei , Zhong Xiangli , Gu Heng , Li Lin 
Published: April 2020

Abstract
Although homogeneous, high quality graphene can be fabricated on a Cu or Ni sheet using the traditional chemical vapour deposition method at high temperatures (over 1000 °C) under specific atmospheric conditions, their transfer to another substrate is difficult. In this paper a novel method of rapidly (i.e. 3–6 s of laser irradiation) producing a large area (>3 cm2) graphene film from olive oil on a glass surface (pre-coated with a 5–28 nm thick Ni film) with defocused, large area continuous laser irradiation is described. The turbostratic graphene film (6 layers) grown in such a way has shown high electrical conductivity (sheet resistance of around 20 Ω sq−1) and an optical transmittance of 40–50%. With femtosecond laser patterning, 70% optical transparency was demonstrated. Continuous large area graphene was formed at relatively lower temperatures (<250 °C) and without the need for specific atmospheric conditions. The basic process characteristics and mechanisms involved are discussed.
Unrecognized Hemoglobin Variants in the Donor Blood Supply Are Detectable in the Transfused Population
Authors: Rutherford-Parker Nicola J , Colby Jennifer M , Gehrie Eric A , Booth Garrett S 
Published: April 2020

AbstractObjectivesThe US Food and Drug Administration requires donated blood to be tested for various infectious diseases to ensure safety and purity. However, testing for hemoglobin variants is not required, despite reported occurrences of hemoglobin variant transfusion and concerns about the safety of such transfusions. This study aimed to investigate the frequency of hemoglobin variants within the blood supply.MethodsWe performed a 2-part study. First, we tested all RBC units in our blood bank by high-performance liquid chromatography for the presence of hemoglobin variants. Second, we performed a retrospective analysis of hemoglobin variant testing completed for routine management of sickle cell disease patients at our institution over a 5-month period to identify cases of hemoglobin variant transfusion.ResultsWe found that 2 of 476 (0.4%) RBC units in our blood bank contained a hemoglobin variant, and 5 of 563 (0.9%) sickle cell patients seen at our institution in a 5-month period were transfused with RBCs containing a hemoglobin variant.ConclusionsWe confirmed that hemoglobin variants are present within the blood supply, and the frequency of hemoglobin variant transfusion is elevated for patients with sickle cell disease given the increased prevalence of hemoglobin variants in the population of matched donors.
Obstetric oxytocin exposure and ADHD and ASD among Danish and Finnish children
Authors: Stokholm Lonny , Juhl Mette , Talge Nicole M , Gissler Mika , Obel Carsten , Strandberg-Larsen Katrine 
Published: April 2020

Abstract

Background
Some studies have indicated an increased risk of attention deficit hyperactivity disorder (ADHD) and a small, sex-specific association with autism spectrum disorder (ASD) among children prenatally exposed to obstetric oxytocin. Since oxytocin is widely used in the obstetric ward, these potentially deleterious effects are of concern. Thus, we aimed to examine whether obstetric oxytocin treatment for labour induction or augmentation is associated with ADHD and ASD in offspring born in a two-country design based on data from Denmark and Finland.

Methods
This population-based study used data from national registers in Denmark and Finland. Singletons born in Denmark 2000–10 (n = 577 380) and Finland 1991–2010 (n = 945 543), who survived infancy, were followed until 31 December 2015. ADHD and ASD were defined using diagnostic codes. For ADHD, we also included information on prescribed and redeemed ADHD medication in the definition. Hazards ratios (HRs) with 95% confidence intervals (CI), modelled with age as the underlying time scale, were calculated to estimate the associations.

Results
Oxytocin was used in 31% and 46% of the included deliveries in Denmark and Finland, respectively. In crude analyses, prenatal oxytocin was associated with an approximately 20% increased risk of ADHD and ASD, but confounder adjustment attenuated the association. The adjusted HR was 1.03, 95% CI 1.01–1.05, for ADHD and 1.05, 95% CI 1.02–1.08, for ASD. The results were similar in across country and gender.

Conclusions
We found an association between synthetic oxytocin and ADHD or ASD which is unlikely to reflect a causal association and thus should not support the concern of clinical use. Our results help to allay concerns of obstetric use of oxytocin causing ADHD or ASD.
Experimental Design of Vortex Assisted Switchable Solvent Homogeneous Liquid-Liquid Microextraction for Simultaneous Determination of Four Pesticides in Wastewater
Authors: Tışlı Büşra , Chormey Dotse Selali , Ayyıldız Merve Fırat , Bakırdere Sezgin 
Published: April 2020

Abstract

Background
Pesticides are chemicals used mainly to protect plant crops in order to increase their production efficiency and quality.

Objective
Switchable-solvent homogeneous liquid-liquid microextraction was optimized using a Box-Behnken experimental design and validated on a gas chromatography mass spectrometry system for the determination of analytes.

Method
The significance of independent variables (switchable solvent volume, sodium hydroxide volume, and vortex period) and their interactions were evaluated by analysis of variance at 95% confidence limits (α = 0.05).

Results
The LOD and LOQ ranges of the analytes were found to be 0.42–1.90 µg/L and 1.36–6.33 µg/L, respectively. Percentage recovery results were found to be in the range of 87–113% in spiking experiments.

Conclusions
A simple, efficient, and accurate analytical method was developed for the simultaneous determination of the selected pesticides. Highlights: Matrix matching was used to enhance quantification accuracy for real samples.

Highlights
Matrix matching was used to enhance quantification accuracy for real samples.
Risk of Disordered Eating Behaviors in Adolescents with Type 1 Diabetes
Authors: Rose Meredith , Streisand Randi , Tully Carrie , Clary Lauren , Monaghan Maureen , Wang Jichuan , Mackey Eleanor 
Published: April 2020

Abstract

Objective
Management of type 1 diabetes (T1D) may be difficult for adolescents, who often fail to meet glycemic targets. Adolescents are also at an increased risk for negative affect (i.e., anxiety, depressive symptoms) and are prone to disordered eating behaviors (DEB). This study hypothesized that negative affect would be associated with DEB, self-management, and glycemic control, but that this would be moderated by negative urgency (the urge to engage in impulsive behaviors in response to negative affect), such that this relationship would be significant only for those with higher negative urgency.

Methods
The Eating in Adolescents with T1D Study recruited 100 caregiver-adolescent dyads (55% male youth, 48% Caucasian) to complete questionnaires reporting on the adolescent’s negative affect, negative urgency, DEB, and diabetes management. Glycemic control 3–4 months following survey completion was extracted from the medical record.

Results
A total of 61% of adolescents reported elevated symptoms of anxiety or depression and 25% reported elevated disordered eating symptoms. A total of 81% of adolescents had an A1c level above recommended targets. Negative affect was associated with DEB, suboptimal T1D self-management, and suboptimal glycemic outcomes, moderated by higher levels of negative urgency. Negative affect was associated directly with suboptimal self-management and glycemic control, but not DEB, at all levels of negative urgency.

Conclusions
Adolescents reported high levels of negative affect, DEB, and suboptimal glycemic control. Interventions that target negative affect and negative urgency in adolescents with T1D are needed in order to reduce the risk for DEB and negative diabetes health outcomes.
Multifaceted roles of HEAT SHOCK PROTEIN 90 molecular chaperones in plant development
Authors: Tichá Tereza , Samakovli Despina , Kuchařová Anna , Vavrdová Tereza , Šamaj Jozef 
Published: April 2020

AbstractHEAT SHOCK PROTEINS 90 (HSP90s) are molecular chaperones that mediate correct folding and stability of many client proteins. These chaperones act as master molecular hubs involved in multiple aspects of cellular and developmental signalling in diverse organisms. Moreover, environmental and genetic perturbations affect both HSP90s and their clients, leading to alterations of molecular networks determining respectively plant phenotypes and genotypes and contributing to a broad phenotypic plasticity. Although HSP90 interaction networks affecting the genetic basis of phenotypic variation and diversity have been thoroughly studied in animals, such studies are just starting to emerge in plants. Here, we summarize current knowledge and discuss HSP90 network functions in plant development and cellular homeostasis.
miRViz: a novel webserver application to visualize and interpret microRNA datasets
Authors: Giroux Pierre , Bhajun Ricky , Segard Stéphane , Picquenot Claire , Charavay Céline , Desquilles Lise , Pinna Guillaume , Ginestier Christophe , Denis Josiane , Cherradi Nadia , Guyon Laurent 
Published: April 2020

AbstractMicroRNAs (miRNAs) are small non-coding RNAs that are involved in the regulation of major pathways in eukaryotic cells through their binding to and repression of multiple mRNAs. With high-throughput methodologies, various outcomes can be measured that produce long lists of miRNAs that are often difficult to interpret. A common question is: after differential expression or phenotypic screening of miRNA mimics, which miRNA should be chosen for further investigation? Here, we present miRViz (http://mirviz.prabi.fr/), a webserver application designed to visualize and interpret large miRNA datasets, with no need for programming skills. MiRViz has two main goals: (i) to help biologists to raise data-driven hypotheses and (ii) to share miRNA datasets in a straightforward way through publishable quality data representation, with emphasis on relevant groups of miRNAs. MiRViz can currently handle datasets from 11 eukaryotic species. We present real-case applications of miRViz, and provide both datasets and procedures to reproduce the corresponding figures. MiRViz offers rapid identification of miRNA families, as demonstrated here for the miRNA-320 family, which is significantly exported in exosomes of colon cancer cells. We also visually highlight a group of miRNAs associated with pluripotency that is particularly active in control of a breast cancer stem-cell population in culture.
Arabidopsis PsbP-Like Protein 1 Facilitates the Assembly of the Photosystem II Supercomplexes and Optimizes Plant Fitness under Fluctuating Light
Authors: Che Yufen , Kusama Shoko , Matsui Shintaro , Suorsa Marjaana , Nakano Takeshi , Aro Eva-Mari , Ifuku Kentaro 
Published: April 2020

Abstract
In green plants, photosystem II (PSII) forms multisubunit supercomplexes (SCs) containing a dimeric core and light-harvesting complexes (LHCs). In this study, we show that Arabidopsis thaliana PsbP-like protein 1 (PPL1) is involved in the assembly of the PSII SCs and is required for adaptation to changing light intensity. PPL1 is a homolog of PsbP protein that optimizes the water-oxidizing reaction of PSII in green plants and is required for the efficient repair of photodamaged PSII; however, its exact function has been unknown. PPL1 was enriched in stroma lamellae and grana margins and associated with PSII subcomplexes including PSII monomers and PSII dimers, and several LHCII assemblies, while PPL1 was not detected in PSII–LHCII SCs. In a PPL1 null mutant (ppl1-2), assembly of CP43, PsbR and PsbW was affected, resulting in a reduced accumulation of PSII SCs even under moderate light intensity. This caused the abnormal association of LHCII in ppl1-2, as indicated by lower maximal quantum efficiency of PSII (Fv/Fm) and accelerated State 1 to State 2 transitions. These differences would lower the capability of plants to adapt to changing light environments, thereby leading to reduced growth under natural fluctuating light environments. Phylogenetic and structural analyses suggest that PPL1 is closely related to its cyanobacterial homolog CyanoP, which functions as an assembly factor in the early stage of PSII biogenesis. Our results suggest that PPL1 has a similar function, but the data also indicate that it could aid the association of LHCII with PSII.
Regional neurodegeneration correlates with sleep–wake dysfunction after stroke
Authors: Gottlieb Elie , Egorova Natalia , Khlif Mohamed S , Khan Wasim , Werden Emilio , Pase Matthew P , Howard Mark , Brodtmann Amy 
Published: April 2020

Abstract
Sleep–wake disruption is a key modifiable risk factor and sequela of stroke. The pathogenesis of poststroke sleep dysfunction is unclear. It is not known whether poststroke sleep pathology is due to focal infarction to sleep–wake hubs or to accelerated poststroke neurodegeneration in subcortical structures after stroke. We characterize the first prospective poststroke regional brain volumetric and whole-brain, fiber-specific, white matter markers of objectively measured sleep–wake dysfunction. We hypothesized that excessively long sleep (&gt;8 h) duration and poor sleep efficiency (&lt;80%) measured using the SenseWear Armband 3-months poststroke (n = 112) would be associated with reduced regional brain volumes of a priori-selected sleep–wake regions of interest when compared to healthy controls with optimal sleep characteristics (n = 35). We utilized a novel technique known as a whole-brain fixel-based analysis to investigate the fiber-specific white matter differences in participants with long sleep duration. Stroke participants with long sleep (n = 24) duration exhibited reduced regional volumes of the ipsilesional thalamus and contralesional amygdala when compared with controls. Poor sleep efficiency after stroke (n = 29) was associated with reduced ipsilesional thalamus, contralesional hippocampus, and contralesional amygdala volumes. Whole-brain fixel-based analyses revealed widespread macrostructural degeneration to the corticopontocerebellar tract in stroke participants with long sleep duration, with fiber reductions of up to 40%. Neurodegeneration to subcortical structures, which appear to be vulnerable to accelerated brain volume loss after stroke, may drive sleep–wake deficiencies poststroke, independent of lesion characteristics and confounding comorbidities. We discuss these findings in the context of the clinicopathological implications of sleep-related neurodegeneration and attempt to corroborate previous mechanistic-neuroanatomical findings.
Principles of Reinsurance Contract Law Project: enhancing the value of reinsurance cover—viewpoint of an industry practitioner
Authors: Kuitunen Lari 
Published: April 2020

Abstract
This short article discusses the benefits of the Principles of Reinsurance Contract Law project (PRICL) from an insurance industry perspective. The scope is fairly limited, in that this article focuses on changes in the regime of remedies available for contractual breach, particularly for pre-contractual non-disclosure, as well describing the underlying legal uncertainty when relying on industry custom as a source of law in reinsurance. In both of these areas, the PRICL stand to bring improvements to the industry, some dimensions of which are discussed in greater detail in this article by first describing a simple framework for value generation of reinsurance, where reduction in operational risks and lighter regime of remedies (proportional to the severity of the breach of duty), particularly in situations of pre-contractual non-disclosure, reduce the risk of reinsurance covers not responding at a time of need. This improves the certainty of cover and, ultimately, the value of reinsurance covers.
Metabolic interplay and flexibility in the intracellular Trypanosoma cruzi‐host cell interaction
Authors: Burleigh Barbara A. 
Published: April 2020

The mammalian stages of the protozoan parasite and causative agent of Chagas disease, Trypanosoma cruzi, exhibit a wide host species range and extensive within‐host tissue distribution. These features, coupled with the ability of T. cruzi to persist for the lifetime of its mammalian host, suggest an inherent capacity of the parasite to respond flexibly to local changing environments. To explore this potential, the population‐level response of intracellular T. cruzi amastigotes to various metabolic perturbations was examined by measuring proliferation rates and cell cycle dynamics. Intracellular amastigotes responded to transient perturbations with a rapid and reversible suppression of proliferation rates, that in most instances, was accompanied by the reversible accumulation of parasites in the G1 phase of the cell cycle. Further, our examination of the impact of the immediate metabolic environment on the susceptibility of intracellular T. cruzi amastigotes to trypanocidal drugs lead to the finding that a single change in the composition of the cell culture medium, restriction of glutamine, protects intracellular T. cruzi amastigotes from the lethal effects of the ergosterol biosynthesis inhibitors, ketoconazole and posaconazole. The implications of this finding will be discussed along with the results of ongoing molecular genetic experiments designed to elucidate the metabolic basis of this finding.This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.
Somatostatin Receptor Subtype‐4 Agonist Increases Microglia Amyloid‐beta 1‐42 Uptake
Authors: Polina Jahnavi , Walters Field , Sandoval Karin , Crider Albert M. , Witt Ken , Schober Joseph 
Published: April 2020

This research evaluated the somatostatin receptor subtype‐4 (SSTR4) agonist, NNC 26‐9100, on microglial amyloid‐beta 1‐42 (Aβ42) uptake with inflammatory stimulation. Microglia mediated events have been identified as critical to Alzheimer's disease (AD) progression. Considering microglia have been shown to express SSTR4 and have the capacity to take up and degrade hallmark Aβ isoforms, we hypothesized that a SSTR4 agonist may increase beta amyloid uptake dependent upon the level of inflammation.The effect of NNC 26‐9100 was evaluated in immortalized BV2 microglia cells against inflammatory activation using lipopolysaccharide (LPS). BV2 cells were plated at a density of 62,500 cells/cm2 in 12 well plates for 24hrs. FITC‐Aβ42 (100nM) uptake was then assessed following 24hr NNC 26‐9100 treatment (0, 1μM) against increasing concentrations of LPS (0, 0.83, 8.3 ng/mL) by flow cytometry. A two‐way ANOVA with Tukey post‐hoc tests were utilized to assess the impact of NNC 26‐9100, LPS and their interaction on FITC‐Aβ42 uptake. Both LPS (p<0.0001) and NNC 26‐9100 (p=0.0004) significantly affected mean FITCA‐β42 uptake by BV2 cells. While no significant interaction was found between LPS and NNC 26‐9100 on mean FITC‐Aβ42 uptake (p=0.2963), FITC‐Aβ42 uptake significantly increased with each additional increase in LPS (p<0.0001, Tukey). Moreover, FITC‐Aβ42 uptake significantly increased with 1 μM NNC 26‐9100 treatment when compared to vehicle control (p=0.0004, Tukey).In conclusion, the SSTR4 agonist NNC 26‐9100 and LPS significantly increased microglial uptake of Aβ42. These results identify a novel mechanism for enhancing microglial uptake of Aβ42 by an SSTR4 agonist under inflammatory conditions.Support or Funding InformationThis work is supported by the National Institutes of Health, National Institute of Aging (R01AG047858)This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.
Systematic Review of Recent Pediatric Down Syndrome Neuropsychology Literature: Considerations for Regression Assessment and Monitoring
Authors: Grealish Katherine G. , Price August M. , Stein David S. 
Published: April 2020

ABSTRACT:

Objectives:
To conduct a systematic review of recent pediatric Down syndrome (DS) neuropsychology research that may be useful to clinicians and researchers examining regression in this population.

Methods:
We reviewed original peer-reviewed articles published between 2013 and 2018 studying neuropsychological profiles in DS.

Results:
Thirty-one articles (of 1231 included in the original search) passed all inclusion criteria, were evaluated for bias, and were included in the analysis.

Conclusion:
Findings argued against a single “DS profile” and revealed multiple within-group differences as well as expected and unexpected differences relative to typically developing children and children with other intellectual and developmental disabilities. Areas identified as most germane to regression monitoring included working memory, inhibition, letter and word identification, navigational route learning, motor skills (when strong at baseline), single word receptive/expressive vocabulary, and adaptive function.
Questioning a Previous Autism Spectrum Disorder Diagnosis: Can You “Lose” the Diagnosis?
Authors: Zaro Christopher , Jeon Hyejin , Harstad Elizabeth , Conrad Cassandra , Solomon Dasha , Augustyn Marilyn 
Published: April 2020

CASE:
Heidi is an almost 6-year-old girl presenting to your primary care office to establish care because of a change in insurance status. You review her previous medical records before seeing her.
She was diagnosed with autism spectrum disorder (ASD) when she was 25 months old. Her parents were initially concerned about language delay. Through a comprehensive evaluation by a developmental-behavioral pediatrician and a child psychologist, including administration of the Bayley Scales of Infant and Toddler Development and the Autism Diagnostic Observation Schedule, she was diagnosed with ASD. Her cognitive skills were reported to be within the average range. Soon after the diagnosis, she began receiving 20 hours of applied behavioral analysis (ABA) per week, as well as music therapy, occupational therapy, and a toddler playgroup through early intervention. Four months after the initial diagnosis, her parents reported that she had started making small improvements in her behavior, used more eye contact, and seemed more socially engaged. Approximately 1 year after the diagnosis, she was receiving 6 hours of ABA per week in addition to starting preschool with an Individualized Education Program. She reportedly continued to show progress with social communication and pretend play skills.
At the age of 3 years, 8 months, neuropsychological testing was completed at her parent's request, and her cognitive skills and adaptive skills were reported to be within the average range. She continued to meet the diagnostic criteria for ASD, given her challenges with social awareness, communication, delayed play skills, decreased flexibility, and tendency toward subtle self-direction. She continued to receive speech/language therapy and attended an integrated preschool program within the school district because of her social and communication challenges. She also received ABA 4 hours weekly at home.
During your first visit with Heidi, her parents report that she has continued to make progress in all areas, including social skills. She can engage in imaginary play with her friends, ask strangers questions, and comprehend the perspective of others, and she is no longer “rigid.” She is not receiving services outside of school and is only receiving once weekly speech/language therapy in school. Her parents no longer believe that she meets the criteria for ASD, and they are interested in further evaluation. Her parents ask if it is possible to “lose” the diagnosis of ASD. They also want to know if there are other things to be concerned about for her future. How do you respond?