bluuebunny/crossref_metadata_2025_split huggingface dataset:
Awareness and Attitudes Toward HIV Self-Testing in Northern Thailand
Authors: Shafik Nardeen , Deeb Savana , Srithanaviboonc Kriengkrai , Ayood Pisittawoot , Malasao Rungnapa , Siviroj Penprapa , Musumari Patou Masika , Wood Michele M 
Published: September 2020

Abstract
HIV self-testing (HIVST) was recently introduced in Thailand, but little is known about receptivity among its residents. Because HIV testing is a critical component of HIV prevention, it is important to understand how HIVST is perceived among potential users. The purpose of this study was to examine awareness and attitudes toward HIVST among adults in Northern Thailand. A convenience sample of 403 adult residents of the Sanpatong district, Chiang Mai province was interviewed using a structured questionnaire in 2019. Awareness of HIVST was low (14%), as was the overall HIVST negative attitude score (6.44; possible range of 0-14). The odds of being aware of HIVST were more than twice as high for those with more education compared to those with less (AOR=2.29, 95% CI: 1.22 - 4.30), and roughly half as high for those who expressed HIV stigma compared to those who did not (AOR=0.49, 95% CI: 0.26 - 0.91). Holding negative attitudes towards HIVST also was associated with lower education and expressing HIV stigma, but these relationships disappeared in multivariate analysis. Findings may be used by local health organizations to tailor HIVST education efforts.
Changes of Peripheral Lymphocyte Populations in Patients with Advanced/Recurrent Ovarian Cancer Undergoing Splenectomy During Cytoreductive Surgery
Authors: Chen Wei , Ye Shuang , Wu Yutuan , Pei Xuan , Xiang Libing , Ping Bo , Shan Boer , Yang Huijuan 
Published: September 2020

Abstract
Background: To investigate the change of peripheral lymphocyte subsets after splenectomy during cytoreductive surgery for advanced or recurrent ovarian cancers Methods: We enrolled 83 patients with advanced or recurrent ovarian cancer who underwent cytoreductive surgery. Twenty patients who also underwent splenectomy were assigned to the splenectomy cohort and the rest to the non-splenectomy cohort. Flow cytometry was used to measure the peripheral lymphocyte subsets consisting of T cells, regulatory T cells, natural killer cells, B cells, and activation antigens before and after surgery. Results: There wasn’t any difference in the number and distribution of peripheral lymphocyte subsets between the two cohorts before surgery. We observed elevated levels of T cells (CD3+, CD3+CD8+) in the splenectomy cohort compared to those in the non-splenectomy cohort after surgery, which achieved statistical significance. CD8+CD28+ T cells had a significant decreasing tendency (P=0.011) while CD3+/HLA-DR+ T cells were the opposite (P=0.001) in the splenectomy cohort. The proportion of Tregs (P=0.005) and B cells (P<0.001) including CD3-/HLA-DR+ B cells (P=0.007) increased after surgery, and the absolute number of T cells and NK cells decreased to different extents (P<0.001) in the non-splenectomy cohort. The post-operative percentage of CD8+CD28+ T cells was less than the pre-operative one (P=0.022), which was similar to the splenectomy cohort. Conclusions: The changes in peripheral lymphocyte populations were different between patients with and without splenectomy during cytoreductive surgery for ovarian cancers. T cells were increased and activated in splenectomy cohort, whereas, B cells were increased and activated in non-splenectomy.
Combining Gene Expression Signature With Clinical Features for Survival Stratification of Gastric Cancer
Authors: Sun Qiang , Guo Dongyang , Li Shuang , Xu Yanjun , Jiang Mingchun , Li Yang , Duan Huilong , Zhuo Wei , Liu Wei , Zhu Shankuan , Liu Xiangrui , Wang Liangjing , Zhou Tianhua 
Published: September 2020

Abstract
Background: The AJCC staging system is considered as the golden standard in clinical practice. However, it remains some pitfalls in assessing the prognosis of gastric cancer (GC) patients with similar clinicopathological characteristics. We aim to develop a new clinic and genetic risk score (CGRS) to improve the prognosis prediction of GC patients.Methods: The gene expression profiles of the training set from the Asian Cancer Research Group (ACRG) cohort were used for developing genetic risk score (GRS) by LASSO-Cox regression algorithms. CGRS was established by integrating GRS with clinical risk score (CRS) derived from Surveillance, Epidemiology, and End Results (SEER) database. GRS and CGRS were validated in ACRG validation set and other four independent GC cohorts with different data types, such as microarray, RNA sequencing, and qRT-PCR. Multivariable Cox regression was adopted to evaluate the independence of GRS and CGRS in prognosis evaluation.Results: We established GRS based on a nine-gene signature including APOD, CCDC92, CYS1, GSDME, ST8SIA5, STARD3NL, TIMEM245, TSPYL5, and VAT1. GRS and CGRS dichotomized GC patients into high and low risk groups with significantly different prognosis in four independent cohorts, including our Zhejiang cohort (all HR > 1, all P < 0.001). Both GRS and CGRS were prognostic signatures independent of the AJCC staging system. Receiver operating characteristic (ROC) analysis showed that area under ROC curve of CGRS was larger than that of the AJCC staging system in most cohorts we studied. Nomogram and web tool (http://39.100.117.92/CGRS/) based on CGRS were developed for clinicians to conveniently assess GC prognosis in clinical practice.Conclusions: CGRS integrating genetic signature with clinical features shows strong robustness in predicting GC prognosis, and can be easily applied in clinical practice through the web application.
Peer Support Workers as a Bridge: A Qualitative Study Exploring the Role of Peer Support Workers in the Care of People Who Use Drugs During and After Hospitalization&nbsp;
Authors: Lennox Robin , Lamarche Larkin , O'Shea Tim 
Published: September 2020

Abstract
Background: To describe the key qualities and unique roles of peer support workers in the care of people who inject drugs during and after hospitalization.Methods: We conducted a qualitative study. Key stakeholders were recruited including: people who use drugs who had been hospitalized, healthcare team members, peer support workers, and employers of peer support workers. Data was collected from 2019-2020 using semi-structured interviews that were audio-recorded, transcribed, and analyzed thematically.Results: Fourteen participants were interviewed: 6 people who use drugs who had been hospitalized, 5 healthcare team members, 2 peer support workers, and 1 employer of peer support workers. At the core of the data was the notion of peer workers acting as a bridge to overcome systemic barriers. We found two themes that related to functions of this bridge: advocacy and navigating transitions within the healthcare system. We found two themes for building a strong bridge and making the role of a peer support worker function effectively (training and mentorship, and establishing boundaries). We found three themes involving characteristics of an effective peer worker (intrinsic qualities, contributions of shared experiences, and personal stability). When the bridge works well it ultimately serves to rebuild trust between healthcare providers and people who use drugs.Conclusions: Peer support workers are highly valued by both people who use drugs and members of the healthcare team. Peer support workers act as a bridge between patients and healthcare providers, and are critical in establishing trust, easing transitions in care, and providing unique supports to people who use drugs during and after hospitalization.
The Clinical Sustainability Assessment Tool: Measuring Organizational Capacity to Promote Sustainability in Healthcare
Authors: Malone Sara , Prewitt Kim , Hackett Rachel , Lin John C. , McKay Virginia , Walsh-Bailey Callie , Luke Douglas 
Published: September 2020

Abstract
Background Few validated assessment tools are available to support sustainable implementation, an increasingly recognized need among clinicians. We describe the development of the Clinical Sustainability Assessment Tool, or CSAT, designed to assess factors that contribute to sustainable practices in clinical settings. Methods Fifty participants from clinical and research fields were recruited to brainstorm factors that lead to sustained clinical practices. Once repeated factors were removed, participants sorted remaining factors based on similarity. Using concept mapping analyses, we grouped these factors into meaningful domains and developed an initial tool. We then recruited 126 practicing clinicians to pilot and evaluate the tool. Individuals were recruited from clinical settings across pediatric (53%) and adult (47%) medical and surgical subspecialties, and averaged 11 years in their position. The data were analyzed using confirmatory factor analysis (CFA) to test hypothesized subscale structure in the instrument. We used root mean square error of approximation (RMSEA) and the standardized root mean square residual (SRMR) to assess fit and thus the ability of CSAT to measure the identified domains.Results The concept mapping resulted in seven domains and 47 items. The pilot and CFA resulted in 35 items, five per domain. The RMSEA of 0.084 and the SRMR of 0.075 indicated good fit. The final domains were: engaged staff and leadership, engaged stakeholders, organizational readiness, workflow integration, implementation and training, monitoring and evaluation, and outcomes and effectiveness. Conclusions The CSAT is a new reliable assessment tool which allows for greater practical and scientific understanding of contextual factors that enable sustainable clinical practices over time. The full CSAT instrument is available at https://sustaintool.org.
A qualitative assessment of the sexual-health education, training and service needs of young adults in Tehran
Authors: Sheikhansari Narges , Abraham Charles , Denford Sarah , Eftekhar Mehrdad 
Published: September 2020

Abstract
Background: Sexual Health and Relationships Education (SHRE) provides individuals with the knowledge and skills set which helps them to manage risky behaviors and take informed decisions and to protect themselves against STIs, risky behavior and unintended pregnancy. Such education is minimally provided in Iranian schools and universities and previous research has indicated demand and need for SHRE among different social groups. This study explored Iranian young adults’ sexual health education, training and service needs and ways to improve or augment the existing provision.Design and methods: A qualitative design was employed. Semi-structured interviews were conducted with a sample of 25 young adults who lived in Tehran, Iran and have volunteered to participate in our study. Transcripts were analyzed using thematic analysis. Results: Participants explained their need and demand for sexual health education and healthcare. They highlighted existing barriers such as lack of reliable resources, taboo and cultural barriers and lack of trust and protected confidentiality to gaining sexual health information and seeking related advice and healthcare. This has resulted in ambiguities and misconceptions, including those regarding the cause and transmission of STIs and correct use of contraceptions.They unanimously expressed their dissatisfaction with the limitedly available sexual health education and provided recommendations for an improved provision, including holding mixed gender extracurricular workshops with a comprehensive approach to sexual health and relationships education.Conclusions: There is a clear need and demand for provision of relevant and reliable sexual health and relationships education to young adults, which needs to be addressed in order for young adults to make informed choices and limit their risky sexual behavior.
Understanding the Non-Professional Needs of Early Career Doctors: An Interview-Based Study
Authors: Gurney Tiana , O'Sullivan Belinda , McGrail Matthew , Martin Priya 
Published: September 2020

Abstract
BackgroundSpecialty colleges and health services play a key role in supporting doctors in their early careers, fostering a highly skilled workforce to respond to the community’s healthcare needs. In doing so, they are often well attuned to professional factors, such as long or uncontrolled work hours, but non-professional factors are less acknowledged. This is partly because there is limited research about the non-professional needs of early career doctors, impeding the capacity to tailor a response through structured policy and programs. This study aims to describe the non-professional needs of doctors in their early postgraduate career, including how they intersect with career and training experiences.MethodsSemi-structured interviews were conducted with 32 male and female medical graduates working across all Australian states and territories, spanning a variety of specialty areas and early career stages (up to their 17th postgraduate year). Participants were asked about their career journey to date including non-professional factors related to their experiences.ResultsThe results identified important non-professional needs, that strongly interplayed with career and training experiences, including: children’s education; partner’s career needs; family stability; major life stages; proximity to the extended family; and spending time with immediate family. Results also suggested clear gender differences, with female doctor’s needs orientated to partner work and carer responsibilities, while male doctor’s needs were oriented to spending time with family and meeting the family’s needs. ConclusionsThis study highlights a range of important non-professional needs which should be routinely considered as legitimate needs within health service employment and specialty training arrangements. The non-professional needs appear to vary by gender, location and field of work, and whether doctors have partners, children or encounter major life events in their early career. Inflexible postgraduate training and employment, by both specialty colleges and the employing health service, are likely to create tension for early career doctors in their non-professional lives. Whereas accommodating these needs ensures early career doctors can realise their full potential, fostering participation, satisfaction and completion of various early career work and training stages, with positive consequences for community access.
SH3BP2 Deficiency Ameliorates Murine Systemic Lupus Erythematosus
Authors: Kawahara Kyoko , Mukai Tomoyuki , Iseki Masanori , Nagasu Akiko , Nagasu Hajime , Akagi Takahiko , Tsuji Shoko , Hiramatsu-Asano Sumie , Ueki Yasuyoshi , Ishihara Katsuhiko , Kashihara Naoki , Morita Yoshitaka 
Published: September 2020

Abstract
Background: The adaptor protein Src homology 3 domain-binding protein 2 (SH3BP2) is widely expressed in immune cells, such as myeloid cells, B cells, and T cells. It controls intracellular signaling pathways, including Syk and Src. The present study was undertaken to investigate the role of SH3BP2 in a murine systemic lupus erythematosus model.Methods: For the lupus model, we used Faslpr/lpr mice (C57BL/6 background). Clinical and immunological phenotypes were compared between Faslpr/lpr and SH3BP2-deficient Faslpr/lpr mice. Splenomegaly and renal involvement were assessed in 35-week-old mice. Serum levels of anti-dsDNA antibody and rheumatoid factor were determined using ELISA. Lymphocyte subsets in the spleen were analyzed by flow cytometry. To examine the role of SH3BP2 in specific cells, B cell-specific SH3BP2-deficient lupus mice were generated and analyzed; T cells and bone marrow-derived dendritic cells and macrophages were analyzed in vitro. Results: SH3BP2 deficiency significantly reduced lupus-like phenotypes, presented as splenomegaly, renal involvement, elevated serum anti-dsDNA antibody and rheumatoid factor, and increased splenic B220+CD4-CD8- T cells. Notably, SH3BP2 deficiency in B cells did not rescue the lupus-like phenotypes. Furthermore, SH3BP2 deficiency did not substantially affect the characteristics of T cells and macrophages in vitro. Interestingly, SH3BP2 deficiency suppressed the differentiation of dendritic cells in vitro and reduced the number of dendritic cells in the spleen of the lupus-prone mice.Conclusions: SH3BP2 deficiency ameliorated clinical and immunological manifestations in lupus-prone mice, possibly via targeting dendritic cell differentiation. Modulating SH3BP2 expression could thus provide a novel therapeutic approach to autoimmune diseases.
Variation of IgG N-linked glycosylation profile in diabetic retinopathy
Authors: Wu Zhiyuan , Pan Huiying , Liu Di , Zhou Di , Li Haibin , Tao Lixin , Zhang Jie , Wang Xiaonan , Li Xia , Wang Youxin , Wang Wei , Guo Xiuhua 
Published: September 2020

Abstract
Background: The relationship of IgG glycosylation with diabetes and diabetic nephropathy has been reported, while its role in diabetic retinopathy (DR) remained unclear. We aimed to investigate and validate the association of IgG glycosylation with DR. Methods: We analyzed the IgG N-linked glycosylation profile and identified the specific panel in the discovery population using binary logistics model. Findings were validated in the replication population. The discriminative capacity of IgG glycosylation panel was explored by ROC analysis using cross validation and Brier score. Multiple sensitive analyses were performed on the whole population. Results: 2 IgG glycans (GP15, GP20) and 2 derived traits (IGP32, IGP54) were identified and validated significantly associated with DR (P<0.05), and the adjusted OR were 0.676, 0.671, 1.770, 0.681 in combined population, respectively. The glycosylation panel achieved an average AUC of 0.67 and 0.60 in the discovery and replication population. The association was independent of blood pressure, glucose and lipids, thus improving the ROC and Brier score when the panel added. In addition, the results remained consistent when the controls were re-defined and 1:3 re-matched. Conclusions: IgG glycosylation profile reflecting a pro-inflammatory status were associated with DR. The variation of IgG glycome deserves more attention in the aggravation of diabetes and the underlying mechanism warrants further research.
Pyoderma Gangrenosum-like ulceration as a presenting feature of pediatric Granulomatosis with Polyangiitis
Authors: Oz Rotem Semo , Onajin Oluwakemi , Harel Liora , Tal Rotem , Dallos Tomas , Rosenblatt Adena , Plank Lukas , Wagner-Weiner Linda 
Published: September 2020

Abstract
Background: Granulomatosis with polyangiitis (GPA) is an anti-neutrophilic cytoplasmic antibody-associated vasculitis affecting small to medium-sized vessels and involves most commonly the kidneys and the respiratory tract. Skin involvement can be seen in up to 50% of children with GPA and is the initial presenting symptom in 7.7%. Pyoderma gangrenosum (PG)-like ulcers are rarely described as a skin manifestation in GPA and very few cases have been reported previously in children. Case presentation: We describe 3 new pediatric cases of GPA with PG-like ulcerations. The mean age at first symptom was 15 years. Two patients had PG-like ulceration as their initial presentation; additional symptoms eventually led to the diagnosis of GPA 2-24 months later. In 1 case, proteinase 3 (PR3) was negative when first tested, but converted to positive when systemic symptoms emerged, in the other 2 cases PR3 was positive at presentation. All 3 patients had prominent facial lesions. None of the patients responded to treatment with antibiotics or medications commonly used to manage PG, including corticosteroids and cyclosporine. All patients had excellent responses to rituximab. An electronic database literature review was performed and 4 previously reported cases were identified. We assessed the clinical characteristics, serology, and response to treatment of 4 previously reported and newly diagnosed cases. Conclusion: PG-like ulceration is a rare presentation of pediatric GPA which may precede classic systemic GPA symptoms. The predominance of facial ulcer, granulomatous and neutrophilic inflammation on skin biopsy and lack of response to PG treatments are characteristic of GPA-associated PG-like ulcers. Our review suggests that treatment with rituximab may be needed to improve the skin lesions. Recognizing that PG-like ulcerations can occur in pediatric GPA may result in timely diagnosis, appropriate treatment and improved prognosis.
Lower excitons strong light matter interaction in plasmonic tweezers
Authors: Zou Yunfei , Song Gang , Wang Naien , Yu Li 
Published: September 2020

Abstract
Plasmonic nanocavity has been an excellent platform to study light matter interaction under ambient conditions and within sub-diffraction volumes. However, controlled strong light matter interaction in the plasmonic system has rarely been reported. Here, we design a plasmonic tweezers, which can trap a molecular J-aggregates, and be a plasmonic cavity to investigate the strong light matter interaction. We use finite-difference time-domain methods and Maxwell stress tensor to evaluate the optical response and the trapping performance. With the help of coupled oscillator model and virtual excitons theory, we analyze the strong coupling progress in lower excitons system, we further introduce a `coupling force' parameter to characterize the relationship between the optical force and model volume in the coupling system. The proposed method offers a way to locate a molecular J-aggregates in a plasmonic tweezers for investigating optical force performance and strong light matter interaction.
A cis-Tether Terminator, Linc-GmSTT1, Regulates Transcription Termination via the Linc-GmSTT1-intermolecular Interactome in Soybean&nbsp;
Authors: Song Bo , Luo Tingting , Pang Ze , Zheng Yuhong , Zhao Ming , Fang Xin , Ning Bin , Wu Junjiang , Xu Pengfei , Zhang Shuzhen , Liu Shanshan 
Published: September 2020

Abstract
Soybean β-conglycinin α-subunit is an important allergen that adversely affects the nutritional and processing qualities of soya products. Although inheritance of the α-subunit and the molecular basis of α-null mutations have been studied intensively, the molecular mechanism that regulates α-subunit expression remains unclear. Here, we demonstrated that a long intergenic non-coding RNA, acting as a soybean cis-tether terminator1 (designated Linc-GmSTT1) regulate β-conglycinin α-subunit expression. The Linc-GmSTT1 was mapped in physical proximity of α-subunit CG-α-1 gene and demonstrated to be a crucial element of the convergent alpha-transcription termination unit (alpha-TTU). Ingeniously, by reading through, Linc-GmSTT1 and CG-α-1 gene co-transcribed and subsequently achieve its Cgy-2-locus (confirm α-normal) specific regulation function via Linc-GmSTT1-intermolcular interactome. This work provides a unique model whereby LincRNA regulated the effective transcriptional termination of proximal protein-coding genes which might be a crucial procession protecting it from the silencing machinery in plant.