bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical efficacy of acupoint application of Chinese herbal medicine to prevent acute exacerbation of stable chronic obstructive pulmonary disease: A study protocol for a randomized placebo-controlled trial
Authors: Chuantao Zhang , Hongjing Yang , Wenfan Gan , Xiaohong Xie , Peiyang Gao , Jianying Wu , Jun Chen , Keni Zhao , Wei Xiao , Keling Chen , qingsong Huang 
Published: December 2019

Abstract
Background: Chronic obstructive pulmonary disease (COPD) is a major public health problem that severely affects the quality of life of patients and may even endanger their lives. The key to prevent the progression of COPD is active treatment during the stable phase that prevents and reduces acute exacerbation of COPD. Although modern medicine has achieved significant results in relieving the clinical manifestations of COPD, it is difficult to prevent its progression and acute exacerbation entirely. As one of the classic aspects of acupuncture and moxibustion therapy, acupoint application of Chinese herbal medicine (CHM) can improve the clinical efficacy of Western medicine in treating COPD. To date, however,there is no high-quality clinical trial to assess the effectiveness of CHM acupoint application directly in preventing acute exacerbation of stable COPD.Thus, we designed this randomized placebo-controlled clinical trial to evaluate the long-term efficacy and safety of CHM acupoint application in preventing the acute exacerbation of stable COPD.Methods: The study is a randomized, double-blind, placebo-controlled trial, in which 200 stable COPD patients will be randomly and equally divided into the experimental group or control group. Both groups will undergo standard Western medicine treatment; however, the patients in the experimental group will be also treated with CHM acupoint application, while the control group will be given placebo acupoint application. The duration of the treatment will be 1 month and a follow-up for 11 months. The primary outcome will be the number of acute exacerbation episodes of COPD, and the secondary outcomes will include the lung function, St George's Respiratory Questionnaire, COPD Assessment Test, and 6-Minute Walk Test. A safety assessment will also be performed during the trial.Discussion: The aim of this study is to evaluate the efficacy and safety of CHM acupoint application in preventing acute exacerbation of stable COPD. Our study will provide sound evidence to support the evidence-based medicine of CHM acupoint application as an additional measure in the prevention of acute exacerbation of stable COPD.
Clinical value of peripheral blood monocytes related indicators alone or in combination with T-SPOT.TB in the diagnosis of smear negative pulmonary tuberculosis
Authors: Lyu Xiali , Wang Saisai , Jia Hongyan , Li Zihui , Shu Wei , Kang Wanli , Pan Liping , Zhang Zongde , Li Qi 
Published: December 2019

Abstract
Background To improve the early diagnosis of SNPT, is one of the most important measures to reduce the incidence of pulmonary tuberculosis in China. This paper studied the expression characteristics of monocytes and other indicators in peripheral blood of SNPT, and discussed the clinical value of those indicators alone or in combination with T-SPOT.TB in the diagnosis and differential diagnosis of SNPT. Methods : We collected 134 SNPT patients, 56 lung cancer patients, 95 pneumonia patients (including 51 CAP patients and 44 HAP patients) and 117 HC as research subjects from Beijing Chest Hospital, Capital Medical University from January, 2018 to December, 2018. The relevant indicators of peripheral blood monocytes alone or in combination with T-SPOT.TB of the research subjects were analyzed. Data were analyzed by SPSS 25.0 software. Measurement data were analyzed by t test or z test. Enumeration data were analyzed by Chi-square test. ROC curve and diagnostic accuracy were used to evaluate the clinical value. Results : The percentage of monocytes, ML ratio and the positive rate of T-SPOT.TB in SNPT group were higher than those in heathy control group, lung cancer group and pneumonia group, with statistically significance (P<0.05). In comparison with healthy control group, the sensitivity (98.51%) and accuracy (80.88%) of ML ratio combined with T-SPOT.TB (parallel test) for the diagnosis of SNPT group were increased and the rate of missed diagnosis (1.49%) was decreased. In the differential diagnosis of SNPT and lung cancer, the percentage of monocytes was better than the other three indicators, and the combined detection with T-SPOT.TB ( series test) can reduce the misdiagnosis rate and improve the diagnosis accuracy. In the differential diagnosis of SNPT and pneumonia, percentage of monocytes and ML ratio combined with T-SPOT.TB (series test) can reduce the misdiagnosis rate and improve the diagnosis accuracy. Conclusions: The percentage of monocytes and ML ratio have good value of early auxiliary diagnosis, and the combined diagnosis indicators can make up for the deficiency of T-SPOT.TB in the diagnosis of active tuberculosis, and improve the early diagnosis rate of SNPT and the clinical value of differential diagnosis.
Unveil the transcriptome alteration of POMC neuron in diet-induced obesity
Authors: Lyu Peng , Huang Zhishun , Feng Qingjun , Su Yongfu , Zheng Mengying , Hong Yannv , Cai Xiang , LU Zhonglei 
Published: December 2019

Abstract
Background: Loss of neuron homeostasis in the Arcuate nucleus (ARC) is suggested to be responsible for the development diet-induced-obesity (DIO). We previously reported that loss of Rb1 gene compromised the homeostasis of anorexigenic POMC neurons in ARC and induced obesity in mice.Method: To shed light on how DIO develops, we propose to analyze the transcriptomic alteration of POMC neurons in mice following high fat die (HFD) feeding. We isolated the POMC neurons from established DIO mice and performed transcriptomic profiling on them by RNA-seq.Results: A total of 1,066 genes (628 up-regulated and 438 down-regulated) were identified as differentially expressed genes (DEGs). Pathway enrichment analysis with these DEGs further revealed that ‘cell cycle’, ‘apoptosis’, ‘chemokine signalling’ and ‘sphingolipid metabolism’ pathways were correlated with the development of DIO. Moreover, we validated that the pRb protein, key regulator of ‘cell cycle pathway’, was inactivated by phosphorylation in POMC neurons with HFD feeding. Importantly, reversal of deregulated cell cycle by stereotaxic delivering of the unphosphorylated pRb∆P in ARC significantly meliorated the DIO. Together, our study provides insights into the mechanisms related to the loss of homeostasis of POMC neurons in DIO, and suggests pRb phosphorylation as a potential intervention target to treat DIO. Conclusion: The Arcuate nucleus is the material basis that controlled energy balance and glucose metabolism, which is vulnerable to high-fat-diet (HFD) in diet-induced-obesity (DIO). In this study, we conducted transcriptomic profiling in anorexigenic POMC neurons of ARC with HFD to disclose the underlying mechanisms related with the homeostasis maintenance and the development of DIO. Importantly, we suggest that DIO could be prevented of treated by reversal of the deregulated cell cycle in POMC neurons through targeting pRb phosphorylation. Keywords: High-Fat-Diet (HFD); Diet-Induced Obesity (DIO); POMC neuron; Neuron homeostasis; pRb phosphorylation
Genome-wide identification of ZIP gene family members in wheat
Authors: Li Song , Liu ZiHui , Guo Linlin , Li Hongjie , Nie Xiaojun , Chai Shoucheng , Zheng Weijun 
Published: December 2019

Abstract
Background: The plant ZIP (Zn-regulated, iron-regulated transporter-like protein) transporter family is one of the most essential gene families regulating the uptake, transport and accumulation of microelements, which play important roles in plant growth, development and biofortification. Although the ZIP family has been systematically studied in many plant species, the significance of this family in wheat is not well understood at present. Results: Through a genome-wide search based on the latest wheat reference sequence (IWGSC_V1.1), 58 TaZIP genes were identified. Most of these genes were represented by two to three homoalleles, which were named TaZIP_-A, TaZIP_-B, TaZIP_-D, Protein structure analysis revealed that most TaZIP proteins contain more than six transmembrane (TM) domains and that the distance between TM-3 and TM-4 is variable. Furthermore, the TaZIP proteins clustered into four groups in a phylogenetic tree, and the proteins belonging to the same group shared similar exon-intron structures and conserved motifs. Expression pattern analysis revealed that most TaZIP genes were significantly highly expressed in root, and that nine TaZIP genes were up-regulated at the grain filling stage. When exposed to ZnSO4 and FeCl 3 solutions, TaZIP genes showed different expression patterns, and 16 TaZIP genes were identified as candidate high-affinity Zn transporter genes and 23 as low-affinity Zn transporter genes. Finally, using yeast complementation analysis three TaZIP genes were demonstrated to have the capacity to transport Zn and Fe.Conclusion: This study systematically analyzed the genomic organization, gene structures and expression profiles of TaZIPs. The findings not only provide candidates for further functional analysis, but also contribute to a better understanding of the regulatory roles of ZIPs in wheat.
Unicompartmental versus Tricompartmental Knee Arthroplasty with Continuous Adductor Canal and Femoral Nerve Blocks Analgesic Requirements and Implications for Discharge Readiness
Authors: Sztain Jacklynn , Machi Anthony T. , Madison Sarah J. , Abramson Wendy B. , Monahan Amanda M. , Khatibi Bahareh , Ball Scott T. , Gonzales Francis B. , Donohue Michael C. , Carlson David M. , Ilfeld Brian M. 
Published: December 2019

Abstract
Background: The relative analgesic requirements for tricompartmental (TKA) and unicompartmental (UKA) knee arthroplasty and their effects on discharge readiness remain unexamined when continuous adductor canal and femoral nerve blocks are used for analgesia in the immediate postoperative period. Methods: Data were collected from 2 previously-published clinical trials involving subjects undergoing TKA (n=79) or UKA (n=30) randomized to either an adductor canal or femoral perineural catheter and ropivacaine 0.2% infusion for 2 (UKA) or 3 (TKA) days. Originally, we compared each catheter location (adductor vs. femoral) while holding surgical procedure constant (comparing solely TKAs and solely UKAs). We now compare type of surgical procedure (TKA vs UKA) while holding catheter location (adductor vs. femoral) constant. The primary outcome was the time to attain 4 discharge criteria including pain, opioid requirements, and ambulation/mobilization. Results: For adductor canal catheters, UKA patients reached all 4 discharge criteria in 35 [24—43] hours which was significantly faster than those given TKA who took 55 [43—63] hours (difference: 18h; 95%CI 9 to 28 h; P<0.001). The results were similar for femoral catheters: UKA patients reach all four discharge criteria in 40 [27—58] hours which was significantly faster than those given TKA who took 61 [49—69] hours (difference: 20; 95%CI 4 to 30 h; P=0.009). For both catheter locations, pain scores, opioid requirements, and mobilization endpoints were better with UKA than TKA. Conclusion : UKA induces less pain and requires less opioid than TKA, regardless of perineural catheter location. Consequently, patients who have UKA are ready for discharge sooner.
Irregular bone defect repair via tissue-engineered periosteum approach in rabbit model.
Authors: Zhao Lin , Yu Jia-Jia , Zhang Cangyu , Wang Xiuhui 
Published: December 2019

Abstract
Background As an alternative of bone grafts for defect repair, tissue engineering is much promising for clinical application. In previous studies, we have succeeded in repair of long bone defect with homemade tissue-engineered periosteum (TEP), of which is fabraicated by incorporating osteogenically induced mesenchymal stem cells (MSCs) of rabbits with a scaffold of small intestinal submucosa (SIS).Methods In this study, we are aimed to discuss the feasibility of allogenic irregular bone defect repair with the TEP. Thirty-six rabbits whose scapulas were subtotally resected to establish large irregular bone defects model in allogenic rabbits. The defects were treat respectively with TEP (Group 1, n=12), allogenic deproteinized bone (DPB) (Group 2, n=12) and hybrid of TEP and DPB (Group 3, n=12). At 4, 8, and 12 weeks after surgery, the rabbits were sacrificed, and the implants were harvested. X-ray radiographic and histological examinations were performed.ResultsThe findings suggested that the radiographic score in TEP-DPB hybrided implantation (Group 3) was higher than TEP or DPB grafting only (p<0.05).But that was inconsistent with histological findings, which Group1 appeared to possess significantly higher bone formation than Group 2 (p<0.05) and Group3 has higher new bone volume than that of Group 2 (p<0.05).Conclusion We conclude that TEP is a promising alternative in repair of large irregular bone defect.DPB served as a 3D scaffold in combining TEP could provide mechanical support and shaping guide, but hinder new bone formation via TEP approach due to retard degradation.
Tibetan wild asses (Equus Kiang) were superior to domestic donkeys (Equus Asinus) in terms of the composition and function of gut microbial community
Authors: Liu Hongjin , Zhao Xinquan , Xu Shixiao , Zhao Liang , Han Xueping , Hu Linyong , Xu Tianwei , Zhao Na , Zhang Xiaoling , Li Qi , Chen Dongdong , He Fuquan , Chen Xin 
Published: December 2019

Abstract
Tibetan wild asses (Equus Kiang) are the only wild species of perissodactyls on the Qinghai-Tibetan Plateau, and appears on the International Union for Conversation of Nature (IUCN) 2012 Red List of threatened species. Therefor, understanding the gut microbita composition and function can provide a theoretical for the situ conservation of wild animals in the future. This study analyzed the intestinal microbiota of wild asses and domestic donkeys by high-throughput sequencing of the 16s rDNA regions. No significant difference in alpha diversity was detected between these two groups. Beta diversity showed that the bacterial community structure of wild asses was acutely different from domestic donkeys. At the phylum level, the two dominant phyla of Bacteroidetes and Firmcutes in wild asses were significantly higher than that in domestic donkeys. At the genus level, Ruminococcaceae_NK4A214, Phascolarctobacterium, Coprostanoligenes_group, Lachnospiraceae_XPB1014_group and Akkermansia in wild asses were significantly higher than domestic donkeys. Moreover, statistical comparisons showed that 40 different metabolic pathways exhibited significant differences. Among them, 29 pathways had richer concentrations in wild asses than domestic donkeys, mainly included amino acid metabolism, carbohydrate metabolism, and energy metabolism. Of note, network analysis showed that wild asses harbored a relatively more complex bacterial network than domestic donkeys, possibly reflecting the specific niche adaption of gut bacterial communities through species interactions. The overall results indicated that wild asses were superior to that of domestic in gut bacteria community composition and function, and wild asses should be more suitable to survive in wild than to be domesticated or captive.
Multi-objective optimal water supply scheduling model for an inter-basin water transfer system: the South-to-North Water Diversion Middle Route Project, China
Authors: Ouyang Shuo , Qin Hui , Shao Jun , Lu Jiantao , Bing Jianping , Wang Xuemin , Zhang Rui 
Published: December 2019

Abstract
Inter-basin water diversion reallocates water resources by changing their spatio-temporal distribution characteristics between basins. This can effectively relieve water supply and demand conflicts in regions with water resource reserves shortages. However, building inter-basin water diversion projects obviously reduces the inflow from upstream, leading to increasingly conspicuous conflicts between water diversion outside a basin and water utilization inside the basin. To relieve this conflict and explore the optimal scheme of water resource allocation across river basins, this paper chooses the Middle Route Project of the South-to-North Water Diversion Project on the Hanjiang River as a case study. A water supply scheduling model of Danjiangkou Reservoir is built using an integrated inter-basin diversion draw water reservoir regulation (IDR) model to balance multiple conflicting water demands. In the IDR model there are two types of objective sets: aggregate indicators and process matching degree functions. Moreover, six evaluation indexes are selected to analyze the water resource allocation effect of the optimal scheme. The simulation results indicate that the proposed IDR model in this paper is practicable and efficient for water resource allocation across river basins.
A fé como agente de resiliência frente ao diagnóstico e tratamento de mulheres com câncer de mama
Authors: Nogueira da Silva Fernanda Aparecida , Jeanny da Silva Viviane , Jordão Laport Tamires 
Published: December 2019

O câncer de mama é uma doença causada pela multiplicação desordenada de células da mama, tais células anormais se multiplicam, formando um tumor. O câncer de mama é o tipo de doença mais comum entre as mulheres do mundo. O diagnóstico precoce da doença é de extrema importância, e pode-se afirmar que a forma como encara-se esse diagnóstico é tão importante quanto. O objetivo do presente trabalho é investigar se a presença da fé é uma condição relevante tanto no diagnóstico quanto no tratamento de mulheres com câncer de mama, assim como compreender como a fé está relacionada a resiliência. Este estudo, de abordagem qualitativa, foi realizado por meio de uma pesquisa de campo através de entrevistas com 10 mulheres com câncer de mama do Serviço de Oncologia de um hospital. Os resultados sugerem que uma mulher resiliente pode lançar mão da fé como um agente propulsor dessa resiliência, de modo a oferecer recursos que possibilitem a enfrentar tanto o diagnóstico, como o tratamento do câncer de mama. Espera-se que esse estudo contribua para uma melhor resposta de mulheres que se encontram frente a um diagnóstico e tratamento de câncer de mama. Buscando também resultados satisfatórios que corroborem para o crescimento de pesquisas na área em psicologia.

Palavras-Chave: Câncer de mama; Fé; Resiliência; Psicologia.
A Educação Permanente Como Ferramenta Estratégica na Coordenação em Saúde Mental
Authors: Martins Déborah Leonardo de Souza , Silva Ana Lúcia Abrahão da 
Published: December 2019

Objetivo: Reconhecer os elementos empregados pelos trabalhadores de saúde mental na construção do cuidado, junto aos usuários de um Centro de Atenção Psicossocial Infantil. Metodologia: trata-se de uma pesquisa de abordagem qualitativa que utilizará como referencial teórico-metodológico a Sócioclínica Institucional em sua modalidade de intervenção a análise institucional das práticas profissionais. A pesquisa intervenção será realizada no Centro de Atenção Psicossocial Infanto-Juvenil de um município do estado do Rio de Janeiro, tem como critério de inclusão a participação dos trabalhadores que atuam há mais de um ano no serviço de saúde mental, com vínculo estatutário e critério de exclusão, os trabalhadores que se encontrarem de licença e férias. Os dados serão coletados através da realização de encontros com os trabalhadores e entrevistas narrativas. O tratamento dos dados coletados será realizado por meio da identificação de eixos de análises centrados na socioclinica institucional. Resultados esperados: implantar a Educação Permanente em Saúde como ferramenta estratégica para reflexão crítica do processo de trabalho e desenvolvimento dos trabalhadores de saúde mental do município estudado.
A síndrome do esgotamento profissional (Burnout) como injúria aos direitos trabalhistas: princípios do dano existencial
Authors: Flores Tarcísio da Silva , Santos Couto Pablo Luiz , Vilela Alba Benemérita Alves , Siva Jefferson de Almeida , Pereira Samantha Souza da Costa , Souza Elvira Cavalcanti de 
Published: December 2019

Objetivou-se analisar reflexivamente acerca da injúria aos direitos fundamentais garantidos ao trabalhador brasileiro pela Constituição Federal, na questão ambiental e do dano existencial, com enfoque na relação com a síndrome de Burnout. Trata-se de um estudo teórico-reflexivo sobre o estado da arte das publicações levantadas e da Constituição Federal Brasileira, quanto ao dano existencial e seus aspectos jurídicos trabalhistas. É notório que o ambiente laboral, as relações humanas estabelecidas nele e o assédio moral cotidiano, tem se tornado um dos grandes inimigos contra o direito ao gozo do lazer, bem como a intimidade e a privacidade do trabalhador, configurando-se dano existencial e contribuindo para seu esgotamento mental e físico, o que caracteriza a síndrome de Burnout.
Ebook como Tecnologia Educacional no Ensino do Gerenciamento em Segurança do Escolar com Alergia a proteína do Leite de Vaca
Authors: Andrade Debora Cristina Mendonça de , Brum Ana Karine Ramos 
Published: December 2019

Introdução: Dados da Associação Brasileira de Alergia e Imunologia afirmam que entre 6% e 8% das crianças têm alergias alimentares, e dentre elas a alergia a proteína do leite de vaca (APLV) é a causa mais comum, considerada atualmente um problema de saúde pública. Para criança que apresenta reações alérgicas alimentares graves o ambiente extradomiciliar, representa alto risco para desencadear anafilaxia. Ao analisar este cenário, constata-se a seriedade de ter um enfermeiro na escola. O objetivo do estudo é desenvolver uma tecnologia educacional no formato de Ebook para o ensino do gerenciamento em segurança do escolar APLV na formação profissional dos enfermeiros e profissionais de educação. Método: Estudo descritivo, exploratório, de abordagem qualitativa com desenvolvimento de pesquisa metodológica para elaboração de uma tecnologia educacional. Os participantes serão estudantes de enfermagem de uma Universidade Federal, profissionais de educação de uma escola pública e privada nos municípios de Maricá e Niterói, respectivamente. A pesquisa obteve parecer favorável pelo Comitê de Ética em Pesquisa da Universidade Federal Fluminense. Serão agendadas três oficinas, onde será aplicado um questionário semiestruturado. O tratamento e a análise dos dados serão feitos de forma descritiva, através da análise e classificação do conteúdo. Resultados esperados: Pretende-se trazer benefícios ao ensino, pesquisa e a sociedade, discutindo questões que subsidiarão a reorientação do processo de formação dos enfermeiros, engajando-os para que possam atuar conjuntamente com os profissionais da educação, a fim de alcançar a principal meta que é a qualidade do cuidado e a segurança do escolar com APLV.
Descritores: serviços de saúde escolar, tecnologia educacional, segurança do paciente