bluuebunny/crossref_metadata_2025_split huggingface dataset:
Inequality in healthcare use among older people in Colombia
Authors: Garcia-Ramirez Jorge , Nikoloski Zlatko , Mossialos Elias 
Published: October 2020

Abstract
Background
Since the early 1990s, Colombia has made great strides in extending healthcare coverage to its population. In order to measure the impact of these efforts, it is important to assess whether the introduction of universal health coverage has translated into equitable access to healthcare in the country, particularly for the elderly. Thus, in this study we assessed the inequality in utilization of health services among elderly patients in Colombia. In addition, we identified the determinants of healthcare utilization.

Methods
We analyzed the 2015 Colombian health, well-being and aging study (SABE). To classify determinants of healthcare use into predisposing, enabling and need factors, we employed the Anderson framework of healthcare utilization. Use of outpatient, inpatient and preventive health services constituted the dependent variables. We performed multivariate logistic regressions, estimated concentration indexes (CI) and performed decomposition analyses of the CIs to determine the contribution of various determinants to inequality of healthcare utilization.

Results
The study sample included 23,694 adults over 60-years-old. Wealth quintile, urban dwelling, health insurance type and multimorbidity predicted the utilization of all types of healthcare services except for hospitalization. Aside from inpatient care, pro-rich inequality in utilization of healthcare services was present. Wealth quintile and type of health insurance were the largest contributors to pro-rich inequality in use of preventive services.

Conclusions
While there has been progress in health insurance coverage for the elderly in Colombia, there are still equality challenges in the delivery of healthcare, especially for preventive and outpatient care. These inequalities are driven by individual characteristics such as wealth, urban residence, type of health insurance carried, and presence of multimorbidity. To address this issue, the Colombian health system should extend health insurance coverage to uninsured populations, as well as reduce barriers of access to healthcare services among poorest and the rural population receiving subsidized insurance.
Ketogenic diet aggravates cardiac remodeling in adult spontaneously hypertensive rats
Authors: You Yuehua , Guo Yongzheng , Jia Ping , Zhuang Biaobiao , Cheng Yu , Deng Hongpei , Wang Xiaowen , Zhang Cheng , Luo Suxin , Huang Bi 
Published: October 2020

Abstract
Background
Ketogenic diet (KD) has been proposed to be an effective lifestyle intervention in metabolic syndrome. However, the effects of KD on cardiac remodeling have not been investigated. Our aim was to investigate the effects and the underling mechanisms of KD on cardiac remodeling in spontaneously hypertensive rats (SHRs).

Methods
10-week-old spontaneously hypertensive rats were subjected to normal diet or ketogenic diet for 4 weeks. Then, their blood pressure and cardiac remodeling were assessed. Cardiac fibroblasts were isolated from 1- to 3-day-old neonatal pups. The cells were then cultured with ketone body with or without TGF-β to investigate the mechanism in vitro.

Results
4 weeks of KD feeding aggravated interstitial fibrosis and cardiac remodeling in SHRs. More interestingly, ketogenic diet feeding increased the activity of mammalian target of rapamyoin (mTOR) complex 2 pathway in the heart of SHRs. In addition, β-hydroxybutyrate strengthened the progression of TGF-β-induced fibrosis in isolated cardiac fibroblasts. mTOR inhibition reversed this effect, indicating that ketone body contributes to cardiac fibroblasts via mTOR pathway.

Conclusions
These data suggest that ketogenic diet may lead to adverse effects on the remodeling in the hypertensive heart, and they underscore the necessity to fully evaluate its reliability before clinical use.
Ten important roles for academic leaders in data science
Authors: Moore Jason H. 
Published: October 2020

AbstractData science has emerged as an important discipline in the era of big data and biological and biomedical data mining. As such, we have seen a rapid increase in the number of data science departments, research centers, and schools. We review here ten important leadership roles for a successful academic data science chair, director, or dean. These roles include the visionary, executive, cheerleader, manager, enforcer, subordinate, educator, entrepreneur, mentor, and communicator. Examples specific to leadership in data science are given for each role.
RNA editing in cancer impacts mRNA abundance in immune response pathways
Authors: Chan Tracey W. , Fu Ting , Bahn Jae Hoon , Jun Hyun-Ik , Lee Jae-Hyung , Quinones-Valdez Giovanni , Cheng Chonghui , Xiao Xinshu 
Published: October 2020

AbstractBackgroundRNA editing generates modifications to the RNA sequences, thereby increasing protein diversity and shaping various layers of gene regulation. Recent studies have revealed global shifts in editing levels across many cancer types, as well as a few specific mechanisms implicating individual sites in tumorigenesis or metastasis. However, most tumor-associated sites, predominantly in noncoding regions, have unknown functional relevance.ResultsHere, we carry out integrative analysis of RNA editing profiles between epithelial and mesenchymal tumors, since epithelial-mesenchymal transition is a key paradigm for metastasis. We identify distinct editing patterns between epithelial and mesenchymal tumors in seven cancer types using TCGA data, an observation further supported by single-cell RNA sequencing data and ADAR perturbation experiments in cell culture. Through computational analyses and experimental validations, we show that differential editing sites between epithelial and mesenchymal phenotypes function by regulating mRNA abundance of their respective genes. Our analysis of RNA-binding proteins reveals ILF3 as a potential regulator of this process, supported by experimental validations. Consistent with the known roles of ILF3 in immune response, epithelial-mesenchymal differential editing sites are enriched in genes involved in immune and viral processes. The strongest target of editing-dependent ILF3 regulation is the transcript encoding PKR, a crucial player in immune and viral response.ConclusionsOur study reports widespread differences in RNA editing between epithelial and mesenchymal tumors and a novel mechanism of editing-dependent regulation of mRNA abundance. It reveals the broad impact of RNA editing in cancer and its relevance to cancer-related immune pathways.
PRECIOUS: PREvention of Complications to Improve OUtcome in elderly patients with acute Stroke—statistical analysis plan of a randomised, open, phase III, clinical trial with blinded outcome assessment
Authors: de Jonge Jeroen C. , Woodhouse Lisa J. , Reinink Hendrik , van der Worp H. Bart , Bath Philip M. ,   for the PRECIOUS investigators
Published: October 2020

Abstract
Rationale
Aspiration, infections, and fever are common in the first days after stroke, especially in older patients. The occurrence of these complications has been associated with an increased risk of death or dependency.

Aims and design
PREvention of Complications to Improve OUtcome in elderly patients with acute Stroke (PRECIOUS) is an international, multi-centre, 3 × 2 factorial, randomised, controlled, open-label clinical trial with blinded outcome assessment, which will assess whether prevention of aspiration, infections, or fever with metoclopramide, ceftriaxone, paracetamol, respectively, or any combination of these in the first 4 days after stroke onset improves functional outcome at 90 days in elderly patients with acute stroke.

Discussion
This statistical analysis plan provides a technical description of the statistical methodology and unpopulated tables and figures. The paper is written prior to data lock and unblinding of treatment allocation.

Trial registration
ISRCTN registry ISRCTN82217627. Registered on 22 September 2015. The trial was prospectively registered.
NOn-invasive Vagus nerve stimulation in acute Ischemic Stroke (NOVIS): a study protocol for a randomized clinical trial
Authors: van der Meij Anne , van Walderveen Marianne A. A. , Kruyt Nyika D. , van Zwet Erik W. , Liebler Eric J. , Ferrari Michel D. , Wermer Marieke J. H. 
Published: October 2020

Abstract
Background
Secondary damage due to neurochemical and inflammatory changes in the penumbra in the first days after ischemic stroke contributes substantially to poor clinical outcome. In animal models, vagus nerve stimulation (VNS) inhibits these detrimental changes and thereby reduces tissue injury. The aim of this study is to investigate whether non-invasive cervical VNS (nVNS) in addition to the current standard treatment can improve penumbral recovery and limit final infarct volume.

Methods
NOVIS is a single-center prospective randomized clinical trial with blinded outcome assessment. One hundred fifty patients will be randomly allocated (1:1) within 12 h from clinical stroke onset to nVNS for 5 days in addition to standard treatment versus standard treatment alone. The primary endpoint is the final infarct volume on day 5 assessed with MRI.

Discussion
We hypothesize that nVNS will result in smaller final infarct volumes as compared to standard treatment due to improved penumbral recovery. The results of this study will be used to assess the viability and approach to power a larger trial to more definitively assess the clinical efficacy of nVNS after stroke.

Trial registration
ClinicalTrials.govNCT04050501. Registered on 8 August 2019
Assisted life termination and truth telling to terminally ill patients – a cross-sectional study of public opinions in Israel
Authors: Bodas Moran , Velan Baruch , Kaplan Giora , Ziv Arnona , Rubin Carmit , Peleg Kobi 
Published: October 2020

AbstractBackgroundEnd-of-life decisions are highly complex socio-normative and ethical phenomena. The goal of this study was to provide an assessment of public opinions in Israel concerning aspects of end-of-life decisions.MethodsAn online cross sectional study was performed in February 2020. The primary tool including items pertaining to death assistance and truth telling to patients. A sample of 515 participants representative of the adult Israeli population was obtained.ResultsThe majority of participants (71%) supports telling the entire truth to patients even in harsh conditions. Support for truth telling decreases with affiliation to religion, with as little as 40% support among ultra-orthodox. People with vocational education are the least supportive of truth telling. Concerning doctor assisted death, almost half (49%) of the sample were supportive. Opposition is positively associated with religiosity, with 90% of ultra-orthodox and 58% of religious participants opposing doctor-assisted death, compared to only 18% among seculars. Non-Jews were 3.35 times (95%CI: 1.90, 5.91) more likely to oppose doctor assisted death than Jews (p < .0001). An Interrelationship analysis crossing between attitudes revealed that the largest group (39%) was comprised of participants who support both (“autonomists”).ConclusionsIsraelis are overwhelmingly supportive of truth telling to patients. In contrast, Israeli public opinions on doctor assisted death are divided. For both attitudes, religiousness plays a crucial role as a catalyst for conservatism and opposition to change. Almost a half of the public is also supportive of an autonomist approach that would allow patients to decide on ending their own lives.
A review of jellyfish aggregations, focusing on India’s coastal waters
Authors: Baliarsingh Sanjiba Kumar , Lotliker Aneesh Anandrao , Srichandan Suchismita , Samanta Alakes , Kumar Nimit , Nair T. M. Balakrishnan 
Published: October 2020

AbstractA review of jellyfish aggregations focused on India’s coastal waters was conducted, with the aim to enhance understanding of conducive conditions and subsequent ecological impacts. Jellyfish swarming, as well as their beach strandings, have been reported from many areas of the world—including India’s coastal waters. A variety of natural (winds, tidal fronts, surface currents, water temperature, salinity, turbidity, dissolved oxygen) and anthropogenic (water quality deterioration, overfishing, translocation, habitat modification) factors play pivotal roles in triggering jellyfish aggregations. Jellyfish aggregation events in the forms of their swarming in coastal waters and beach strandings have resulted in ephemeral nuisances such as water quality deterioration, food chain alterations, hindrance in seawater uptake by power plants, clogging of nets during fishing operations, and tourism declines. Several well-known Indian tourist beaches (e.g., Puri, Chennai, Goa, and Mumbai) have experienced beach strandings. Despite recurrence of such events, jellyfishes are relatively less scientifically investigated and monitored in Indian coastal waters. Therefore, it is important to determine the environmental conditions that trigger jellyfish swarming, in order to develop effective monitoring and prediction strategies. This study additionally proposes a conceptual framework towards development of a jellyfish monitoring system for Indian waters using satellite and model data.
Perceived psychosocial health and its sociodemographic correlates in times of the COVID-19 pandemic: a community-based online study in China
Authors: Wang Gan-Yi , Tang Shang-Feng 
Published: October 2020

Abstract
Background
Coronavirus disease 2019 (COVID-19) pandemic has been affecting people's psychosocial health and well-being through various complex pathways. The present study aims to investigate the perceived psychosocial health and its sociodemographic correlates among Chinese community-dwelling residents.

Methods
This cross-sectional survey was carried out online and using a structured questionnaire during April 2020. In total, 4788 men and women with the age range of 11–98 years from eight provinces in eastern, central and western China were included in the analysis. We adopted a tactical approach to capture three key domains of perceived psychosocial health that are more likely to occur during a pandemic including hopelessness, loneliness, and depression. Multiple regression method, binary logistic regression model and variance inflation factor (VIF) were used to conduct data analysis.

Results
Respectively 34.8%, 32.5% and 44.8% of the participants expressed feeling more hopeless, lonely, and depressed during the pandemic. The percentage of all three indicators was comparatively higher among women than among men: hopelessness (50.7% vs 49.3%), loneliness (52.4% vs 47.6%), and depression (56.2% vs 43.8%). Being married was associated with lower odds of loneliness among men (odds ratio [OR] = 0.63, 95% CI: 0.45–0.90). Loneliness was negatively associated with smoking (OR = 0.67, 95% CI: 0.45–0.99) and positively associated with drinking (OR = 1.45, 95% CI: 1.04–2.02). Compared with those in the lowest income bracket (< CNY 10 000), men (OR = 0.34, 95% CI: 0.21–0.55) and women (OR = 0.36, 95% CI: 0.23–0.56) in the highest level of annually housed income (> CNY 40 000) had the lowest odds of reporting perceived hopelessness (OR = 0.35, 95% CI: 0.25–0.48). Smoking also showed negative association with depression only among men (OR = 0.63, 95% CI: 0.43–0.91).

Conclusions
More than one-third of the participants reported worsening in the experience of hopelessness and loneliness, with more than two-fifth of worsening depression during the pandemic compared with before the outbreak. Several socioeconomic and lifestyle factors were found to be associated with the outcome variables, most notably participants' marital status, household income, smoking, alcohol drinking, existing chronic conditions. These findings may be of significance to treat patients and help them recover from the pandemic.
Early year undergraduate researchers’ reflections on the values and perceived costs of their research experience
Authors: Ceyhan Gaye D. , Tillotson John W. 
Published: October 2020

Abstract
Background
Prior research reported that motivational beliefs that individuals attach to specific tasks predict continuing interest and persistence in the task. A motivational approach may be particularly useful for understanding undergraduate students’ engagement with research in their first and second years in college. The current study utilizes the expectancy-value theory of achievement motivation to qualitatively explore how much and in what ways early year undergraduate researchers value their research experience and what kinds of costs they associate with it.

Results
The results revealed that intrinsic value had the highest expression in participants’ motivation to engage in research. The second most expressed value type was the utility value of undergraduate research with regards to obtaining the desired outcomes, and attainment value played the least important role in participants’ motivation to engage in research. Findings also indicated that some of the participants associated a cost(s) to their research experience. The highest mentioned perceived cost was opportunity cost, where participants commented on losing other valued alternatives when engaging in research. Participants commented on the time, effort, or amount of work needed to engage in research, and a few participants commented on the emotional cost associated with their research experience in terms of the fear of failure.

Conclusion
As perceived cost is the least studied in the expectancy-value framework, this study contributes to cost values within college students, particularly about early year undergraduate researchers. The findings of this study can form the basis for future work on exploring ways to increase the values and decrease the costs students experience in their undergraduate research experiences.
Development and fabrication of disease resistance protein in recombinant Escherichia coli
Authors: Effendi Sefli Sri Wahyu , Tan Shih-I , Chang Chien-Hsiang , Chen Chun-Yen , Chang Jo-Shu , Ng I-Son 
Published: October 2020

AbstractCyanobacteria and Spirulina produce C-phycocyanin (CPC), a water soluble protein associated pigment, which is extensively used in food and pharmaceutical industries. Other therapeutic proteins might exist in microalgal cells, of which there is limited knowledge. Such proteins/peptides with antibiotic properties are crucial due to the emergence of multi-drug resistant pathogens. In addition, the native expression levels of such disease resistant proteins are low, hindering further investigation. Thus, screening and overexpression of such novel proteins is urgent and important. In this study, a protein which was identified as a putative disease resistance protein (DRP) in the mixture of Spirulina product has been explored for the first time. To improve protein expression, DRP was cloned in the pET system, co-transformed with pRARE plasmid for codon optimization and was significantly overexpressed in E. coli BL21(DE3) under induction with isopropyl-β-d-1-thiogalactopyranoside (IPTG). Furthermore, soluble DRP exhibited intense antimicrobial activity against predominant pathogens, and an inhibition zone of 1.59 to 1.74 cm was obtained for E. coli. At a concentration 4 mg/mL, DRP significantly elevated the growth of L. rhamnosus ZY up to twofold showing probable prebiotic activities. Moreover, DRP showed potential as an effective antioxidant, and the scavenging ability for ROS was in the order of hydroxyl > DPPH > superoxide radicals. A putative disease resistance protein (DRP) has been identified, sequenced, cloned and over-expressed in E. coli as a functional protein. Thus expressed DRP showed potential anti-microbial and antioxidant properties, with promising therapeutic applications.
Introduction: Knowledge of Birds and Feathers in the Ancient and Colonial Mesoamerican World
Authors: Sousa Lisa , Caplan Allison 
Published: October 2020

AbstractBirds and their feathers have long occupied a unique place in the social, cultural, and intellectual life of the Americas. This was particularly so in Mesoamerica, where ancient civilizations and colonial societies developed extensive knowledge of birds, their behaviors and habitats, and their vibrant plumage. This special issue brings together scholars from a variety of disciplines, including art history, history, and biology, to promote discussion among the arts, social sciences, and natural sciences on the role of birds and feathers in Mesoamerica. This introductory essay first provides a discussion of the major trends in the scholarship on birds and feathers in ancient and colonial Mesoamerica. It then highlights the contributions of the articles in the special issue to our understanding of the multifaceted roles that both symbolic and real birds and their feathers played in indigenous and transatlantic knowledge systems and societies.
Bridging Biology and Ethnohistory: A Case for Collaboration
Authors: Caplan Allison , Maley James M. , McCormack John E. 
Published: October 2020

AbstractInterdisciplinary scholarship that combines research questions and methodologies from biology and ethnohistory generates new insights into historical interactions between human and bird populations in ancient and colonial Mesoamerica. Codices, ethnohistorical sources, and surviving feather art point to the religious, economic, and artistic importance of various types of birds to Nahua people. Alongside the well-known resplendent quetzal and lovely cotinga, many additional species were significant to ancient and colonial Nahuas. This article presents potential directions for scholarship that bridge biology and ethnohistory and surveys key resources, including natural history collections and online databases. Finally, the article employs the biological literature to describe eleven bird species of great importance to Nahuas, detailing the species’ appearance and plumage, geographic range, variation, habitat, behaviors, and current status. Ultimately, the article demonstrates how insights from natural history and ethnohistory together allow for a fuller understanding of Nahuas’ material and conceptual interactions with these birds.
The Tecolotl and the Chiquatli: Omens of Death and Transspecies Dialogues in the Aztec World
Authors: García Garagarza León 
Published: October 2020

Abstract
This essay examines some instances of interspecific dialogues between owls and human beings recorded in Nahuatl-language sources from the sixteenth century. Since ancient times, owls have been considered omens of death in Mexico. This article analyzes the cultural and linguistic context of this belief among the contact-period Nahuas: the import of tetzahuitl (omens) in the animistic worldview of the Aztecs, as well as the characteristic semantic pair in tecolotl, in chiquatli (“the owl, the barn owl”) to signify the lethal activities of the most representative messengers of the Lords of Death and Destiny, Mictlantecuhtli and Tezcatlipoca. Moreover, the essay shows how the ancient Nahuas considered the intelligibility of animal languages and engaged in active dialogues with the animal representatives of the gods, a form of communication that encompassed both the private and public spheres, as in these dialogues matters of disease, pollution, and warfare came into consideration.