bluuebunny/crossref_metadata_2025_split huggingface dataset:
Performance of Two Fentanyl Immunoassays against a Liquid Chromatography–Tandem Mass Spectrometry Method
Authors: Feng Sheng , Rutledge Theodore J , Manzoni Maureen , Le Thuan , Gardiner JoAnn , Milone Michael , Shaw Leslie , Wang Ping 
Published: May 2020

Abstract
Rapid and automated fentanyl screening assays are in need due to the prevalence of fentanyl abuse. In the present study, we evaluated the clinical performance of two FDA-cleared automated fentanyl immunoassays, the Immunalysis SEFRIA fentanyl assay and the ARK fentanyl assay. Liquid chromatography–tandem mass spectrometry (LC–MS-MS) was used as a gold standard. Two groups of urine specimens were tested, including 225 specimens from patients presenting to the emergency department (ED) for whom urine drugs of abuse screens were ordered and 57 specimens from patients in chronic pain management programs. The SEFRIA assay generated higher assay imprecision than ARK assay (intraday CV%, 7.15 vs. 4.7%; interday CV%, 6.6 vs. 5.3%). Clinical sensitivity and specificity for detection of fentanyl exposure were 100 and 96% for the ARK assay and 95 and 80% for the SEFRIA assay. An ‘auto-repeating’ issue was observed for some validation specimens flagged with high absorbance values (OD &gt; 3.0), generating false repeat results. The frequency of auto-repeating was lower in the ARK assay than SEFRIA (0.7 vs. 15.5%). Auto-repeating occurred for only previously frozen specimens in the ARK assay, but 9% of fresh specimens were also flagged and repeated in the SEFRIA assay. Positive predictive value (PPV) of the ARK assay was 73% in the ED population and 67% in the non-ED populations. The concentrations of fentanyl and norfentanyl were higher in specimens from ED patients than patients from pain management programs. High prevalence of morphine, methamphetamine, benzoylecgonine and 6-MAM was observed in specimens positive for fentanyl in both populations.
Racial/Ethnic Disparities in Lost Earnings From Cancer Deaths in the United States
Authors: Zhao Jingxuan , Miller Kimberly D , Islami Farhad , Zheng Zhiyuan , Han Xuesong , Ma Jiemin , Jemal Ahmedin , Yabroff K Robin 
Published: May 2020

AbstractBackgroundLittle is known about disparities in economic burden due to premature cancer deaths by race or ethnicity in the United States. This study aimed to compare person-years of life lost (PYLLs) and lost earnings due to premature cancer deaths by race/ethnicity.MethodsPYLLs were calculated using recent national cancer death and life expectancy data. PYLLs were combined with annual median earnings to generate lost earnings. We compared PYLLs and lost earnings among individuals who died at age 16-84 years due to cancer by racial/ethnic groups (non-Hispanic [NH] White, NH Black, NH Asian or Pacific Islander, and Hispanic).ResultsIn 2015, PYLLs due to all premature cancer deaths were 6 512 810 for NH Whites, 1 196 709 for NH Blacks, 279 721 for NH Asian or Pacific Islanders, and 665 968 for Hispanics, translating to age-standardized lost earning rates (per 100 000 person-years) of $34.9 million, $43.5 million, $22.2 million, and $24.5 million, respectively. NH Blacks had higher age-standardized PYLL and lost earning rates than NH Whites for 13 of 19 selected cancer sites. If age-specific PYLL and lost earning rates for NH Blacks were the same as those of NH Whites, 241 334 PYLLs and $3.2 billion lost earnings (22.6% of the total lost earnings among NH Blacks) would have been avoided. Disparities were also observed for average PYLLs and lost earnings per cancer death for all cancers combined and 18 of 19 cancer sites.ConclusionsImproving equal access to effective cancer prevention, screening, and treatment will be important in reducing the disproportional economic burden associated with racial/ethnic disparities.
Sucrose regulates wall ingrowth deposition in phloem parenchyma transfer cells in Arabidopsis via affecting phloem loading activity
Authors: Wei Xiaoyang , Nguyen Suong T T , Collings David A , McCurdy David W 
Published: May 2020

Abstract
In Arabidopsis thaliana, phloem parenchyma transfer cells (PPTCs) occur in leaf minor veins and play a pivotal role in phloem loading. Wall ingrowth formation in PPTCs is induced by the phloem loading activity of these cells, which is regulated by sucrose (Suc). The effects of endogenous versus exogenous Suc on wall ingrowth deposition, however, differ. Elevating endogenous Suc levels by increased light enhanced wall ingrowth formation, whereas lowering endogenous Suc levels by dark treatment or genetically in ch-1 resulted in lower levels of deposition. In contrast, exogenously applied Suc, or Suc derived from other organs, repressed wall ingrowth deposition. Analysis of pAtSUC2::GFP plants, used as a marker for phloem loading status, suggested that wall ingrowth formation is correlated with phloem loading activity. Gene expression analysis revealed that exogenous Suc down-regulated expression of AtSWEET11 and 12, whereas endogenous Suc up-regulated AtSWEET11 expression. Analysis of a TREHALOSE 6-PHOSPHATE (T6P) SYNTHASE overexpression line and the hexokinase (HXK)-null mutant, gin2-1, suggested that Suc signalling of wall ingrowth formation is independent of T6P and HXK. Collectively, these results are consistent with the conclusion that Suc regulates wall ingrowth formation via affecting Suc exporting activity in PPTCs.
Halting the Vicarious Liability Juggernaut: Barclays Bank PLC v Various Claimants
Authors: Purshouse Craig 
Published: May 2020

Abstract
In Barclays Bank plc v Various Claimants [2020] UKSC 13, the Supreme Court rejected the claimants’ argument that Barclays should be vicariously liable for the sexual assaults of a doctor hired on as a contractor to perform medical examinations on employees and job candidates at the bank. It upheld the traditional rule that a defendant is not vicariously liable for the torts of independent contractors. This commentary examines the law on liability for independent contractors and considers whether the Supreme Court decision is consistent with modern employment trends. The implications of the decision for medical law are then discussed.
Non-ideal magnetohydrodynamics versus turbulence – I. Which is the dominant process in protostellar disc formation?
Authors: Wurster James , Lewis Benjamin T 
Published: May 2020

ABSTRACT
Non-ideal magnetohydrodynamics (MHD) is the dominant process. We investigate the effect of magnetic fields (ideal and non-ideal) and turbulence (sub- and transsonic) on the formation of circumstellar discs that form nearly simultaneously with the formation of the protostar. This is done by modelling the gravitational collapse of a 1 M⊙ gas cloud that is threaded with a magnetic field and imposed with both rotational and turbulent velocities. We investigate magnetic fields that are parallel/antiparallel and perpendicular to the rotation axis, two rotation rates, and four Mach numbers. Disc formation occurs preferentially in the models that include non-ideal MHD where the magnetic field is antiparallel or perpendicular to the rotation axis. This is independent of the initial rotation rate and level of turbulence, suggesting that subsonic turbulence plays a minimal role in influencing the formation of discs. Aside from first core outflows that are influenced by the initial level of turbulence, non-ideal MHD processes are more important than turbulent processes during the formation of discs around low-mass stars.
Apparent evidence for Hawking points in the CMB Sky★
Authors: An Daniel , Meissner Krzysztof A , Nurowski Paweł , Penrose Roger 
Published: May 2020

ABSTRACT
This paper presents strong observational evidence of numerous previously unobserved anomalous circular spots, of significantly raised temperature, in the cosmic microwave background sky. The spots have angular radii between 0.03 and 0.04 rad (i.e. angular diameters between about 3° and 4°). There is a clear cut-off at that size, indicating that each anomalous spot would have originated from a highly energetic point-like source, located at the end of inflation – or else point-like at the conformally expanded Big Bang, if it is considered that there was no inflationary phase. The significant presence of these anomalous spots, was initially noticed in the Planck 70 GHz satellite data by comparison with 1000 standard simulations, and then confirmed by extending the comparison to 10 000 simulations. Such anomalous points were then found at precisely the same locations in the WMAP (Wilkinson Microwave Anisotropy Probe) data, their significance was confirmed by comparison with 1000 WMAP simulations. Planck and WMAP have very different noise properties and it seems exceedingly unlikely that the observed presence of anomalous points in the same directions on both maps may come entirely from the noise. Subsequently, further confirmation was found in the Planck data by comparison with 1000 FFP8.1 MC simulations (with l ≤ 1500). The existence of such anomalous regions, resulting from point-like sources at the conformally stretched-out big bang, is a predicted consequence of conformal cyclic cosmology, these sources being the Hawking points of the theory, resulting from the Hawking radiation from supermassive black holes in a cosmic aeon prior to our own.
High-velocity interstellar absorption associated with the supernova remnant W28
Authors: Ritchey Adam M 
Published: May 2020

ABSTRACT
We present an analysis of moderately high-resolution optical spectra obtained for the sightline to CD−23 13777, an O9 supergiant that probes high-velocity interstellar gas associated with the supernova remnant W28. Absorption components at both high positive and high negative velocity are seen in the interstellar Na i D and Ca ii H and K lines towards CD−23 13777. The high-velocity components exhibit low Na i/Ca ii ratios, suggesting efficient grain destruction by shock sputtering. High column densities of CH+, and high CH+/CH ratios, for the components seen at lower velocity may be indicative of enhanced turbulence in the clouds interacting with W28. The highest positive and negative velocities of the components seen in Na i and Ca ii absorption towards CD−23 13777 imply that the velocity of the blast wave associated with W28 is at least 150 km s−1, a value that is significantly higher than most previous estimates. The line of sight to CD−23 13777 passes very close to a well-known site of interaction between the supernova remnant and a molecular cloud to the north-east. The north-east molecular cloud exhibits broad molecular line emission, OH maser emission from numerous locations, and bright extended GeV and TeV γ-ray emission. The sightline to CD−23 13777 is thus a unique and valuable probe of the interaction between W28 and dense molecular gas in its environs. Future observations at ultraviolet and visible wavelengths will help to better constrain the abundances, kinematics, and physical conditions in the shocked and quiescent gas along this line of sight.
Cosmology-insensitive estimate of IGM baryon mass fraction from five localized fast radio bursts
Authors: Li Z , Gao H , Wei J-J , Yang Y-P , Zhang B , Zhu Z-H 
Published: May 2020

ABSTRACT
Five fast radio bursts (FRBs), including three apparently non-repeating ones, FRB 180924, FRB 181112, and FRB 190523, and two repeaters, FRB 121102 and FRB 180916.J0158+65, have already been localized so far. We apply a method developed recently by us to these five localized FRBs to give a cosmology-insensitive estimate of the fraction of baryon mass in the intergalactic medium, fIGM. Using the measured dispersion measure (DM) and luminosity distance dL data (inferred from the FRB redshifts and dL of Type Ia supernovae at the same redshifts) of the five FRBs, we constrain the local $f_{\rm IGM} = 0.84^{+0.16}_{-0.22}$ with no evidence of redshift dependence. This cosmology-insensitive estimate of fIGM from FRB observations is in excellent agreement with previous constraints using other probes. Moreover, using the three apparently non-repeating FRBs only we get a little looser but consistent result: $f_{\rm IGM} = 0.74^{+0.24}_{-0.18}$. In these two cases, reasonable estimations for the host galaxy DM contribution (DMhost) can be achieved by modelling it as a function of star formation rate. The constraints on both fIGM and DMhost are expected to be significantly improved with the rapid progress in localizing FRBs.
HomolWat: a web server tool to incorporate ‘homologous’ water molecules into GPCR structures
Authors: Mayol Eduardo , García-Recio Adrián , Tiemann Johanna K S , Hildebrand Peter W , Guixà-González Ramon , Olivella Mireia , Cordomí Arnau 
Published: May 2020

Abstract
Internal water molecules play an essential role in the structure and function of membrane proteins including G protein-coupled receptors (GPCRs). However, technical limitations severely influence the number and certainty of observed water molecules in 3D structures. This may compromise the accuracy of further structural studies such as docking calculations or molecular dynamics simulations. Here we present HomolWat, a web application for incorporating water molecules into GPCR structures by using template-based modelling of homologous water molecules obtained from high-resolution structures. While there are various tools available to predict the positions of internal waters using energy-based methods, the approach of borrowing lacking water molecules from homologous GPCR structures makes HomolWat unique. The tool can incorporate water molecules into a protein structure in about a minute with around 85% of water recovery. The web server is freely available at http://lmc.uab.es/homolwat.
Identification of a novel nucleolar protein complex required for mitotic chromosome segregation through centromeric accumulation of Aurora B
Authors: Fujimura Akiko , Hayashi Yuki , Kato Kazashi , Kogure Yuichiro , Kameyama Mutsuro , Shimamoto Haruka , Daitoku Hiroaki , Fukamizu Akiyoshi , Hirota Toru , Kimura Keiji 
Published: May 2020

AbstractThe nucleolus is a membrane-less nuclear structure that disassembles when cells undergo mitosis. During mitosis, nucleolar factors are thus released from the nucleolus and dynamically change their subcellular localization; however, their functions remain largely uncharacterised. Here, we found that a nucleolar factor called nucleolar protein 11 (NOL11) forms a protein complex with two tryptophan-aspartic acid (WD) repeat proteins named WD-repeat protein 43 (WDR43) and Cirhin in mitotic cells. This complex, referred to here as the NWC (NOL11-WDR43-Cirhin) complex, exists in nucleoli during interphase and translocates to the periphery of mitotic chromosomes, i.e., perichromosomal regions. During mitotic progression, both the congression of chromosomes to the metaphase plate and sister chromatid cohesion are impaired in the absence of the NWC complex, as it is required for the centromeric enrichment of Aurora B and the associating phosphorylation of histone H3 at threonine 3. These results reveal the characteristics of a novel protein complex consisting of nucleolar proteins, which is required for regulating kinetochores and centromeres to ensure faithful chromosome segregation.
Orthogonal tuning of gene expression noise using CRISPR–Cas
Authors: Wu Fan , Shim Jiyoung , Gong Ting , Tan Cheemeng 
Published: May 2020

Abstract
The control of gene expression noise is important for improving drug treatment and the performance of synthetic biological systems. Previous work has tuned gene expression noise by changing the rate of transcription initiation, mRNA degradation, and mRNA translation. However, these methods are invasive: they require changes to the target genetic components. Here, we create an orthogonal system based on CRISPR-dCas9 to tune gene expression noise. Specifically, we modulate the gene expression noise of a reporter gene in Escherichia coli by incorporating CRISPR activation and repression (CRISPRar) simultaneously in a single cell. The CRISPRar uses a single dCas9 that recognizes two different single guide RNAs (sgRNA). We build a library of sgRNA variants with different expression activation and repression strengths. We find that expression noise and mean of a reporter gene can be tuned independently by CRISPRar. Our results suggest that the expression noise is tuned by the competition between two sgRNAs that modulate the binding of RNA polymerase to promoters. The CRISPRar may change how we tune expression noise at the genomic level. Our work has broad impacts on the study of gene functions, phenotypical heterogeneity, and genetic circuit control.
Crossing the Cervicothoracic Junction During Posterior Cervical Fusion for Myelopathy Is Associated With Superior Radiographic Parameters But Similar Clinical Outcomes
Authors: Chan Andrew K , Badiee Ryan K , Rivera Joshua , Chang Chih-Chang , Robinson Leslie C , Mehra Ratnesh N , Tan Lee A , Clark Aaron J , Dhall Sanjay S , Chou Dean , Mummaneni Praveen V 
Published: May 2020

Abstract

Background
For laminectomy and posterior spinal fusion (LPSF) surgery for cervical spondylotic myelopathy (CSM), the evidence is unclear as to whether fusions should cross the cervicothoracic junction (CTJ).

Objective
To compare LPSF outcomes between those with and without lower instrumented vertebrae (LIV) crossing the CTJ.

Methods
A consecutive series of adults undergoing LPSF for CSM from 2012 to 2018 with a minimum of 12-mo follow-up were identified. LPSF with subaxial upper instrumented vertebrae and LIV between C6 and T2 were included. Clinical and radiographic outcomes were compared.

Results
A total of 79 patients were included: 46 crossed the CTJ (crossed-CTJ) and 33 did not. The mean follow-up was 22.2 mo (minimum: 12 mo). Crossed-CTJ had higher preoperative C2-7 sagittal vertical axis (cSVA) (33.3 ± 16.0 vs 23.8 ± 12.4 mm, P = .01) but similar preoperative cervical lordosis (CL) and CL minus T1-slope (CL minus T1-slope) (P > .05, both comparisons). The overall reoperation rate was 3.8% (crossed-CTJ: 2.2% vs not-crossed: 6.1%, P = .37). In adjusted analyses, crossed-CTJ was associated with superior cSVA (β = –9.7; P = .002), CL (β = 6.2; P = .04), and CL minus T1-slope (β = –6.6; P = .04), but longer operative times (β = 46.3; P = .001). Crossed- and not-crossed CTJ achieved similar postoperative patient-reported outcomes [Visual Analog Scale (VAS) neck pain, VAS arm pain, Nurick Grade, Modified Japanese Orthopedic Association Scale, Neck Disability Index, and EuroQol-5D] in adjusted multivariable analyses (adjusted P > .05). For the entire cohort, higher postoperative CL was associated with lower postoperative arm pain (adjusted Pearson's r –0.1, P = .02). No postoperative cervical radiographic parameters were associated with neck pain (P > .05).

Conclusion
Subaxial LPSF for CSM that crossed the CTJ were associated with superior radiographic outcomes for cSVA, CL, and CL minus T1-slope, but longer operative times. There were no differences in neck pain or reoperation rate.
Optical and X-ray observations of stellar flares on an active M dwarf AD Leonis with the Seimei Telescope, SCAT, NICER, and OISTER
Authors: Namekata Kosuke , Maehara Hiroyuki , Sasaki Ryo , Kawai Hiroki , Notsu Yuta , Kowalski Adam F , Allred Joel C , Iwakiri Wataru , Tsuboi Yohko , Murata Katsuhiro L , Niwano Masafumi , Shiraishi Kazuki , Adachi Ryo , Iida Kota , Oeda Motoki , Honda Satoshi , Tozuka Miyako , Katoh Noriyuki , Onozato Hiroki , Okamoto Soshi , Isogai Keisuke , Kimura Mariko , Kojiguchi Naoto , Wakamatsu Yasuyuki , Tampo Yusuke , Nogami Daisaku , Shibata Kazunari 
Published: May 2020

AbstractWe report on multi-wavelength monitoring observations of an M-dwarf flare star AD Leonis with the Seimei Telescope (6150–7930 Å), SCAT (Spectroscopic Chuo-university Astronomical Telescope; 3700–7500 Å), and NICER (Neutron Star Interior Composition Explorer; 0.2–12.0 keV), with the collaboration of the OISTER (Optical and Infrared Synergetic Telescopes for Education and Research) program. Twelve flares are detected in total, including ten Hα, four X-ray, and four optical-continuum flares; one of them is a superflare with a total energy of ∼2.0 × 1033 erg. We found that: (1) during the superflare, the Hα emission line full width at 1/8 maximum dramatically increases to 14 Å from 8 Å in the low-resolution spectra (R ∼ 2000) accompanied by large white-light flares, (2) some weak Hα/X-ray flares are not accompanied by white-light emissions, and (3) the non-flaring emissions show clear rotational modulations in X-ray and Hα intensity in the same phase. To understand these observational features, one-dimensional hydrodynamic flare simulations are performed using the RADYN code. We find the simulated Hα line profiles with hard and high-energy non-thermal electron beams to be consistent with the initial phase line profiles of the superflares, while those with a softer and/or weak-energy beam are consistent with those in decay phases, indicating the changes in the energy fluxes injected to the lower atmosphere. Also, we find that the relation between the optical continuum and Hα intensity is nonlinear, which can be one cause of the non-white-light flares. The flare energy budget exhibits diversity in the observations and models, and more observations of stellar flares are necessary for constraining the occurrence of various emission line phenomena in stellar flares.
Long-term systemic glucocorticoid therapy and weight gain: a population-based cohort study
Authors: Fardet Laurence , Nazareth Irwin , Petersen Irene 
Published: May 2020

Abstract

Objectives
To describe the variation in weight gain in people chronically exposed to systemic glucocorticoids in primary care and to identify the risk factors for weight gain.

Methods
Data were analysed from the British database, The Health Improvement Network. Body weight variations of individuals prescribed systemic glucocorticoids for at least 3 months at a mean dose ≥10 mg/day were described. The risk factors associated with weight gain ≥10% of the usual weight were assessed.

Results
A total of 31 516 adults prescribed glucocorticoids and 26 967 controls were included in the study. During glucocorticoid exposure, only 12 475 (39.6%) individuals gained &gt;2 kg compared with their usual weight. Younger women were more likely to gain weight (mean weight gain in 18–39-year-old glucocorticoid-exposed women: 3.6 kg (s.d. 8.6) compared with 2 kg (s.d. 7.3) in the control group; the absolute mean difference was 1.6 kg (95% CI 0.9, 2.2; P &lt; 0.001). Weight gain ≥10% of the usual weight was observed in 10.2% (n = 3208) of those chronically exposed to glucocorticoids. Women, younger people, those living in areas of higher deprivation, smokers, those on higher doses of the drug and those previously exposed to glucocorticoids were at higher risk. The risk was lower in people prescribed glucocorticoids for an inflammatory condition when compared with asthma or chronic obstructive pulmonary disease.

Conclusion
After taking into account usual weight rather than weight just before glucocorticoid initiation and the natural history of weight variation, the amount of weight gain induced by systemic glucocorticoids as prescribed in primary care is less than usually thought.

Clinical trial registration
18THIN081.