bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mesenchymal stem/stromal cell quality control: validation of mixed lymphocyte reaction assay using flow cytometry according to ICH Q2(R1)
Authors: Nicotra Tess , Desnos Aurélie , Halimi Justine , Antonot Hélène , Reppel Loïc , Belmas Thomas , Freton Alice , Stranieri Floriane , Mebarki Miryam , Larghero Jérôme , Cras Audrey , Faivre Lionel 
Published: October 2020

Abstract
Background
Mesenchymal stem/stromal cells (MSC) have immunomodulatory properties, studied in a wide range of diseases. Validated quality controls must confirm this activity in the context of clinical trials. This study presents a method’s validation, assessing MSC’s ability to inhibit lymphocyte proliferation, according to the ICH Q2 standard.

Methods
MSC were co-cultured with CellTrace™ Violet-labeled Peripheral blood mononuclear cells (PBMC) coming from a bank of ten donors, at seven different ratios for 7 days. Cell trace violet PBMC bank was validated in parallel. Flow cytometry analysis was used to obtain the division percentage of T cells. The percentage of inhibition of lymphocyte proliferation by MSC, for each ratio X, was calculated using the formula: Ratio × percentage of inhibition = (control percentage of division—ratio × percentage of division)/control percentage of division. The inhibition percentage of lymphocyte proliferation function of co-culture ratios was represented in a line graph. The corresponding area under the curve was calculated, representing MSC’s ability to inhibit lymphocyte proliferation.

Results
Two cell trace violet PBMC banks were compared for bank validation. When compared using four different MSC samples coming each from a different donor, their area under the curve did not show any statistical differences and were correlated. Moreover, the stability of one cell trace violet PBMC bank was confirmed up to 509 days of storage.
Analytical parameters were investigated for method validation. Analysis of repeatability and reproducibility respectively showed a standard deviation of 6.1% and 4.6%. The assay was robust regarding PBMC, as no statistical differences were found between inhibitory activities when testing three adjacent concentrations of PBMC. Still, attention is needed on MSC quantity as it can influence results. Linearity was evaluated: the percentage of inhibition of lymphocyte proliferation function of co-culture ratios was linear on the exploited range. Finally, the assay measurement range allowed to differentiate MSC presenting different inhibition activities.

Conclusion
This quantification method displayed low analytical variability and no inter-bank variability of PBMC. However, MSC quantification should be checked before co-culture to reduce variability. Therefore, it could be used for the qualification of MSC batches’ immunomodulatory activity.
Current status and future perspectives of HLA-edited induced pluripotent stem cells
Authors: Koga Keiko , Wang Bo , Kaneko Shin 
Published: October 2020

AbstractIn 2007, Human-induced pluripotent stem cells (iPSCs) were generated by transducing four genes (Oct3/4, Sox2, Klf4, c-Myc). Because iPSCs can differentiate into any types of cells in the body and have fewer ethical issues compared to embryonic stem (ES) cells, application of iPSCs for regenerative medicine has been actively examined. In fact, iPSCs have already been used for clinical applications, but at present, only autologous iPSC-derived grafts or HLA homozygous iPSC-derived grafts are being transplanted into patients following HLA matching. HLA is an important molecule that enables the immune system differentiates between self and non-self-components; thus, HLA mismatch is a major hurdle in the transplantation of iPSCs. To deliver inexpensive off-the-shelf iPSC-derived regenerative medicine products to more patients, it is necessary to generate universal iPSCs that can be transplanted regardless of the HLA haplotypes. The current strategy to generate universal iPSCs has two broad aims: deleting HLA expression and avoiding attacks from NK cells, which are caused by HLA deletion. Deletion of B2M and CIITA genes using the CRISPR/Cas9 system has been reported to suppress the expression of HLA class I and class II, respectively. Transduction of NK inhibitory ligands, such as HLA-E and CD47, has been used to avoid NK cell attacks. Most recently, the HLA-C retaining method has been used to generate semi-universal iPSCs. Twelve haplotypes of HLA-C retaining iPSCs can cover 95% of the global population. In future, studying which types of universal iPSCs are most effective for engraftment in various physiological conditions is necessary.
Study on the Optical Luminescence Properties of Li2TiO3: Mn4+ and Cr3+
Authors: Novita Mega , Marlina Dian , Ogasawara Kazuyoshi , Seok Kim Jeong , Soo Kim Yang 
Published: October 2020

Abstract
We studied the optical luminescence properties of Li2TiO3: Mn4+ based on first-principles calculations. The optical luminescence properties such as Mn-O distance, molecular orbital (MO) energy, crystal field splitting, multiplet energy levels, and Coulomb interaction are discussed in detail. The impact of lattice relaxation was examined through geometry optimizations based on first-principles band-structure calculations using Cambridge Serial Total Energy Package (CASTEP) software. Both Discrete Variational-Xα (DV-Xα) and the Discrete Variational Multi-electron (DVME) software were used to estimate the optical luminescence properties. Here we also predicted the optical luminescence properties of Li2TiO3: Cr3+. The results imply that the multiplet energy levels tend to decline in the order of Mn4+ to Cr3+. The integral Coulomb's decreasing tendency is in excellent agreement with the declining tendency of 2E, 2T1, 2T2 energies of Li2TiO3: Mn4+ and Li2TiO3: Cr3+.
Imaging quality and prostate MR: it is time to improve
Authors: Giganti Francesco , Allen Clare 
Published: October 2020

The Prostate Imaging Reporting and Data System (PI-RADS) guidelines set out the minimal technical requirements for the acquisition of multiparametric MRI (mpMRI) of the prostate. However, the rapid diffusion of this technique has inevitably led to variability in scan quality among centres across the UK and the world. Suboptimal image acquisition reduces the sensitivity and specificity of this technique for the detection of clinically significant prostate cancer and results in clinicians losing confidence in the technique.
Two expert panels, one from the UK and one from the European Society of Urogenital Radiology (ESUR)/EAU Section of Urologic Imaging (ESUI), have stressed the importance to establish quality criteria for the acquisition of mpMRI of the prostate. A first attempt to address this issue has been the publication of the Prostate Imaging Quality (PI-QUAL) score, which assesses the mpMRI quality against a set of objective criteria (PI-RADS guidelines) together with criteria obtained from the image.
PI-QUAL represents the first step towards the standardisation of a scoring system to assess the quality of prostate mpMRI prior to reporting and allows clinicians to have more confidence in using the scan to determine patient care. Further refinements after robust consensus among experts at an international level need to be agreed before its widespread adoption in the clinical setting.
Evaluation of the eating habits of breast cancer patients
Authors: Kayan Tapan Tuba , Iyigun Zeynep Erdogan , Ilgun Serkan , Ozmen Vahit 
Published: October 2020

Objective: To determine the relationship between the dietary characteristics of breast cancer patients.
Methods: Patients with breast cancer whose treatments have finished and are in remission formed the study group and healthy people formed the control group. Demographic, anthropometric characteristics, food consumption frequency form and exercise status were recorded with all groups. Data analysis was done by SPSS 22.
Results: In the study group, mean carbohydrate percentage was lower, while fat, fat percentage, monosaccharide, glucose, fructose, omega3(n3), saturated fatty acids(SFA), monounsaturated fatty acids (MUFA), vitamin A, C, E, B6, biotin and copper values were significantly higher (p<0.05). Recurrence was observed in seven patients (7.1%) during the follow-up period, hormone receptor levels (ER) and vitamin B2 intake (accuracy 93.9%) were inversely related to the recurrence of the disease (p=0.02).
Conclusions: While the percentage of carbohydrate taken was lower in study group; total fat, n3, SFA, MUFA, monosaccharide, glucose, fructose, water-soluble fiber, B6, biotin and copper values were higher. Further studies are needed for vitamin B2 deficiency in patients with recurrence.
doi: https://doi.org/10.12669/pjms.36.7.2368
How to cite this:Tapan TK, Iyigun ZE, Ilgun S, Ozmen V. Evaluation of the eating habits of breast cancer patients. Pak J Med Sci. 2020;36(7):1562-1566. doi: https://doi.org/10.12669/pjms.36.7.2368
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Staying home: teledentistry in the delivery of paediatric dental care
Authors: Marshall Sophie , Kandiah Thayalan 
Published: October 2020

Virtual clinics were introduced at Surrey and Sussex Healthcare NHS Trust in 2019 as an innovative way to improve access to care for patients. During the COVID-19 pandemic, these clinics have been key to the delivery of care to patients. This article discusses the applications and patient reported outcomes of the use of teledentistry before the COVID-19 pandemic, examines the applications of virtual clinics during the COVID-19 pandemic, and considers the role of teledentistry in routine care and patient management during the COVID-19 recovery phase. Applications in teaching are also considered along with limitations and considerations for setting up teledentistry services. Is ‘healing at a distance’ a viable solution? We investigate the benefits and limitations of virtual clinics in managing young patients.
Phased return to dental services during the delay phase of the COVID-19 pandemic
Authors: Rodriguez Jose , Kalsi Harpoonam 
Published: October 2020

A high number of patients in primary and secondary care had their dental treatments cancelled following the suspension of all primary care routine dental services in late March 2020 in response to the COVID-19 pandemic. This article outlines a protocol which can be used to classify patients according to their clinical needs and their COVID-19 risk to prioritise appointments appropriately during the delay phase of the COVID-19 pandemic. Patients should be classified into low or high clinical risk, and low or high COVID-19 risk. Both scores are entered into a matrix which gives a score that can be used to prioritise their needs. From the scores, it is possible to determine which treatments can be delayed, which patients need virtual consultations, and which patients need to be seen urgently. The scoring system can be used in primary and secondary care, across all dental specialties, and allows efficient allocation of resources, ensuring that patients with urgent clinical needs are prioritised accordingly. Classifying patient risk using a priority scoring system will allow for appropriate workforce distribution and allocation of dental appointments.
True grit? Learning points from a facial soft tissue wound
Authors: Cole-Healy Zachary , Broderick Damian , M Graham Richard 
Published: October 2020

We write this short piece regarding a recently referred case to our oral and maxillofacial surgery (OMFS) unit, which highlights several important learning points for OMFS junior staff. We describe a pertinent case in which the importance of history, examination and relevant investigations during the workup of soft tissue facial injuries is illustrated. Fundamentally, all facial traumatic wounds require a thorough assessment that is performed with a high index of suspicion of foreign body implantation. This case report highlights that all facial traumatic wounds require a thorough assessment performed with a high index of suspicion for foreign body implantation.
High Number of Species of Social Wasps (Hymenoptera, Vespidae, Polistinae) Attests the Great Biodiversity of Western Amazon: a Survey from Rondônia, Brazil.
Authors: Gomes Bruno , Lima Caio Souza , Da Silva Marjorie , Noll Fernando Barbosa 
Published: October 2020

A survey of social wasps (Vespidae, Polistinae), common insects of Neotropical fauna, which performs a great variety of important ecosystemic services, was conducted for the first time in areas of the Amazon forest in Rondônia state. The state is part of the western Amazon, a region harboring high biodiversity, but which has been suffering from constant deforestation. Three areas were sampled, and the wasps were actively collected, and an attractive liquid was sprayed onto the vegetation to bait the wasps. A total of 2961 wasps were sampled in all three areas, distributed in 72 species of 15 genera. Thirty-nine species were recorded for the first time in the state and three others (Agelaia melanopyga Cooper, Brachygastra cooperiAndena and Carpenter and Polybia diguetana du Buysson)represents the first record for Brazil.AgelaiaLepeletier was the most abundant genus in all areas, which is congruent with results of other surveys, and the greatest species richness was found for Polybia Lepeletier. The highest number of species was sampled in Floresta Nacional do Jamari (51), followed by Estação Ecológica de Cuniã (46) and forest fragment of Universidade Federal de Rondônia (39). The latter also presented the highest number of different genera sampled. The great diversity sampled, mainly for Epiponini, which represented 64 of the 72 species collected, can be attributed to various factor as the location of the areas and also to the methodology used.
Non-Inseminated Queens Have Worker-Like Behaviors in Colonies of Fungus-Growing Ants, Mycetomoellerius turrifex Wheeler (Attini, Hymenoptera)
Authors: Murakami Takahiro 
Published: October 2020

In eusocial Hymenoptera such as ants, bees, and wasps, the queen numbers are fundamentally important to maintain the social systems. In Texas, USA, a fungus growing ant, Mycetomoellerius turrifex Wheeler (1903) (the genus name was changed from Trachymyrmex to Mycetomoellerius in 2019 (Solomon et al., 2019)) was observed to have several non-inseminated queens that wandered outside the nest long after the mating season. However, the evolutionary and ecological factors causing the occurrence of such non-inseminated queens are still unclear. Thus, I examined the worker-like behaviors of non-inseminated queens of M. turrifex in Texas. Fifteen ant colonies were collected over three years, between 1999 and 2001. The frequencies of non-inseminated queens, workers, and broods, as well as the depths of nest chambers, were observed in each collecting year. In Nov. 1999 and May 2000, multiple nests contained queens that did not mate with males within their nests. These nests had a relatively larger colony size than those collected in Oct. 2001. Conversely, the colonies collected in Oct. 2001 were completely monogynous, i.e., there were no non-inseminated queens in the nests. Behavioral observations of each female revealed that the non-inseminated queensbehaved significantly differently from the workers and the inseminated queens. The behaviors that distinguished different female castes were mutualistic fungus garden care, digging of nest floors, guarding, and resting in the nest. These data suggest that queens failed to mate due to severe environmental fluctuations in southern Texas, but were accepted by the colony as a temporary labor force.
EFEK AROMATERAPI MINYAK ATSIRI MAWAR (Rosa damascena MILL.) DAN KULIT JERUK LIMAU (Citrus amblycarpa) TERHADAP JUMLAH MIKROBA UDARA RUANGAN BERPENDINGIN
Authors: Komala Oom , Utami Novi Fajar , Rosdiana Siti Mariyam 
Published: October 2020

Energy, material, and or other components were entered to the air by activities of human could cause air quality down to a specific level that could affect human health. The bacteria in the human oral cavity such as Staphylococcus sp. were found in the air during common activities such as coughing, sneezing, and speaking. The research aimed to assess the influence of the combination of oils essential of Rosa damacena and leather oil of Citrus amblycarpa against a decline in the total of microbes on air-conditioned rooms. The method of the research was used the evaporation of aromatherapy and plate count method. The results were showed that P3 with the concentration of oils essential of R. damacena 2% and leather oil of C. amblycarpa 5% most effective could decrease the total of microbes with an average of 72.5%. Organoleptic, relative density, refractive index, solubility in 90% ethanol, and acid number analysis of Rosa damacena and Citrus amblycarpa leather essential oils met the Indonesian National Standard requirements except for the acid number of leatherlime essential oil and the optical rotation of rose essential oil. It is concluded that the combination of oils essential R. damacena and leather oil of C. amblycarpa could reduce the total of microbes on air-conditioned rooms.
HUBUNGAN PANJANG-BOBOT DAN FAKTOR KONDISI IKAN NILEM (Osteochilus vittatus VALENCIENNES, 1842) DI PERAIRAN WADUK BENANGA, KALIMANTAN TIMUR
Authors: Jusmaldi Jusmaldi , Hariani Nova , Wulandari Nikmahtulhaniah Ayu 
Published: October 2020

Biology of bonylip barb (Osteochilus vittatus Valenciennes, 1842) in Benanga Reservoir, East Kalimantan is not yet known. The purpose of this study was to analyze distribution of the length and body weight of fishes, sex ratio, gonad maturity level, length-body weight relationship, and condition factor. In total 278 fishes samples were collected during the five months, from January to May 2019 with a purposive sampling method at Benanga Reservoir, and gill net with mesh size 0.5; 1; and 1.5 inches used. The results of this research showed that the total length of the fishes analyzed ranged from 72.21–196.57 mm and body weight ranged from 5.12–89.21 g. The sex ratio of male and female were 1:2.02. The male and female fishes were dominated by mature gonad. The length-body weight relationship models calculated for the total fishes sample was W=1x10-5L2.967 (r=0.988) for males, W=5x10-6L3.199 (r=0.989) for females and W=7x10-6L3.126 (r=0.987) for both sexes. Isometric growth patterns (b=3) were observed in males, meanwhile positive allometric growth patterns (b>3) were observed in females and both sexes. Calculation of the condition factor (K) resulted in 1.181±0.117 for males, 0.949±0.094 for females, and 1.026±0.150 for all specimens. The condition factors of bonylip barb from the Benanga Reservoir inside the range recommended as suitable for freshwater fish species in the tropical regions.
PENGARUH MEDIA TERKONDISI SEL PUNCA MESENSIMAL TERHADAP EKSPRESI GEN TRANSCRIPTION FACTOR 7-LIKE 2 (TCF7L2) TIKUS MODEL DIABETES MELITUS TIPE 2
Authors: Widhiastuti Stefani Santi , Branitamahisi Bernadia , Inayati Nor Sri , Preharsini Ida Ayu , Handika Demas Bayu , Sadewa Ahmad Hamim , Laqif Abdurahman , Haryana Sofia Mubarika 
Published: October 2020

Diabetes mellitus type 2 is the most common type of diabetes. This study was conducted to determine the effect of Mesenchymal Stem Cell-Conditioned Medium (MSC-CM) in Homeostatic Model Assessment of β-cell function (HOMA-β) value, normal Langerhans cells, and Transcription Factor 7-Like 2 (TCF7L2) gene expression in type 2 diabetic rats model. As many as 27 male Sprague Dawley rats were divided into 3 study research groups: normal control (9 normal rats), diabetic control (9 type 2 diabetic rats, induced by 60 mg/kg BW Streptozotocin and 120 mg/kg BW Nicotinamide i.p.), and treatment (9 type 2 diabetic rats treatment with 0.1 ml/200g BW MSC-CM i.p.). On day 30 after therapy, the expression of TCF7L2 gene was performed with real time-quantitative PCR (RT-qPCR). The HOMA-β value were calculated based on Fasting Insulins (FINs) levels and Fasting Blood Glucose (FBG) levels data from other research team members. Based on results, MSC-CM increases the HOMA-β value and amount of normal Langerhans cells of treatment group that indicates amelioration effect of MSC-CM, but there was no significant difference in TCF7L2 gene expression level between diabetic control and treatment group.
JUMLAH, UJI VIABILITAS DAN DAYA KECAMBAH POLEN 31 AKSESI PISANG (Musa sp.) KOLEKSI KEBUN PLASMA NUTFAH PISANG LIPI
Authors: Hasrianda Erwin Fajar , Zaelani Ahmad , Poerba Yuyu Suryasari 
Published: October 2020

Cross pollination is one of the plant breeding techniques to create a better cultivar with desired traits. A successful cross pollination is affected by quality and quantity of the pollen used. The aim from this study is to know amounts, viability and germination from various banana accessions. This study was conducted by using 31 banana accessions. Pollen quantity per anther, pollen viability, pollen germination growth were measured. The results showed that pollen amounts were ranged between 5.032 and 118.181 pollens per anther. In addition, pollen viability ranged from 30% in Tingalun accession (Musa sp. cv. Tingalun) to 99% in Malaccensis 2x accession. Furthermore, pollen germination level varied from 1% in Mambee Thu OS accession to 48% in Rutilifes 2x accession. We also found that negative correlation between pollen viability and pollen germination were moderate with correlation coeeficient value of -0,59 ( p-value = 0,00051). From 31 banana accessions tested in this research, pollen source from Malaccensis 2x and Rutilifes 2x banana accessions have the highest potential to be used as the male parent.
VEGETASI POHON DAN PERSEBARANNYA DI TAMAN WISATA ALAM GUNUNG TUNAK DAN HUTAN KERAMAT, MANDALIKA, LOMBOK TENGAH, PROVINSI NUSA TENGGARA BARAT
Authors: Mansur Muhammad 
Published: October 2020

The study was conducted on March 2018 in two locations study, i.e. Mount Tunak Nature Tourism Park (NTP) (6400 m2 plot area) and Keramat Forest (4800 m2 plot area), Central Lombok District, West Nusa Tenggara Province. The objective of the study was to find out the diversity of tree species and its distribution which can be used for forest area management by the local government and can support the establishment of a Special Economic Region in Mandalika as a place for conservation, research, education and scientific tourism. In the total plot of 1.12 hectare, we recorded 1560 individuals, representing 56 species, 47 genera and 19 families. All stems ≥ 5 cm in diameter were measured and identified. In the plot of Mt. Tunak NTP, we recorded 992 individuals, representing 41 species, basal area was 11.4 m2, 31.7 m3of estimated bole volume, 78.3 tons biomass, 39.2 tons carbon stock and 143.7 tons CO2 sequestration. Five dominant tree species (trunk diameter ≥ 5 cm) recorded with the highest Importance Value Index (IVI) were Aglaia argentea (IVI= 34.3%), Schoutenia ovata (IVI= 33.3%), Drypetes neglecta (IVI= 25.4%), Glycosmis pentaphylla (IVI= 16.4%) and Grewia koordersiana (IVI= 14.9%). The level of diversity of tree species in the Mt. Tunak NTP was classified into a midle level with a Diversity Index (H')= 3.3, while in the Keramat Forest we recorded 568 individuals, representing 37 species, basal area was 19.9 m2, 51.1 m3 of estimated bole volume, 149.8 tons biomass, 74.9 tons carbon stock and 274.9 tons CO2 sequestration. Five dominant tree species were recorded; Dalbergia latifolia (IVI= 69.4%), Grewia koordersiana (IVI= 34.5%), Schoutenia ovata (IVI= 34.3%), Cupaniopsis macropetala (IVI= 18.2%) and Rhus succedanea (IVI= 14.2%). The level of diversity recorded (H')= 2.9 and classified as midle level. The composition of vegetation in Mt.Tunak NTP and Keramat Forest is different (Jaccard Similarity Index was 39.3%).