bluuebunny/crossref_metadata_2025_split huggingface dataset:
Characterization of tebuconazole resistance in Botrytis cinerea from tomato plants in China
Authors: Zhang Can , Li Tengjiao , Xiao Lu , Zhou Shunli , Liu Xili 
Published: August 2020

AbstractFungal pathogen Botrytis cinerea, the casual agent of gray mold of vegetables and fruits, has a high risk of developing resistance to fungicide. Tebuconazole, one kind of demethylation inhibitor (DMI) fungicides, has been increasingly applied for the control of tomato gray mold in China. However, very limited information is available on the resistance profile of B. cinerea to tebuconazole. In this study, the baseline sensitivity of B. cinerea to tebuconazole was determined based on 138 isolates from field sites having no history of DMI usage, with a mean EC50 value of 0.29 μg/mL. Another 159 B. cinerea isolates collected in the greenhouse and field from 2011 to 2016 were demonstrated to have a shifted sensitivity to tebuconazole, with a mean EC50 value of 0.66 μg/mL. EC50 values of 10 B. cinerea isolates with reduced sensitivity to tebuconazole were greater than 1.56 μg/mL, and these reduced-sensitive isolates had a fitness penalty in sporulation and conidial germination, but showed similar mycelial growth rate and pathogenicity with those of the sensitive isolates. Positive cross-resistance was observed only between tebuconazole and the other two DMIs difenoconazole and prochloraz, but not between tebuconazole and the non-DMIs iprodione, procymidone, or fludioxonil. In reduced-sensitive isolates, no amino acid variation was found in the BcCYP51 protein. When exposed to tebuconazole, the expression level of BcCYP51 increased in these reduced-sensitive B. cinerea isolates as compared to sensitive ones, thus contributing to the reduced sensitivity of the pathogen to tebuconazole. Additionally, the nucleotide mutation observed in the 1200 bp upstream region of BcCYP51 had no correlation with the development of fungicide resistance in B. cinerea isolates. These findings will be helpful for the understanding of DMI resistance mechnism in B. cinerea.
Effect of socioeconomic factors on malnutrition among children in Pakistan
Authors: Ahmad Dilshad , Afzal Muhammad , Imtiaz Aisha 
Published: August 2020

Abstract
Background
In the current era, malnutrition among children considers main reason of morbidity and mortality in the world scenario while more specifically in developing countries. Malnutrition in children severely affects their physical growth and academic achievements. This study aimed to find out the effect of socioeconomic factors on malnutrition, children aged under 5 years in Multan district of Punjab province, Pakistan. The study used the data of 2497 children, obtained from Multiple Integrated Cluster Survey 2018 and employed Binary logistic regression approach for empirical estimation the effects of socioeconomic factors on malnutrition among children.

Results
Empirical estimates of the study specified the prevalence of higher frequency of stunting (18.58), wasting (28.43) and underweight (19.54) among children particularly in rural areas of the study district Multan. Stunting status was significantly associated with children of some month (OR = 1.247, CI 95% 0.543–1.546), family size (OR = 0.589, CI 95% 0.431–3.715), maternal education (OR = 1.432, CI 95% 0.528–1.972), wealth quintile (OR = 2.174, CI 95% 1.234–3.376) and sanitation facility (OR = 0.789, CI 95% 0.627–1.987). Wasting status was mostly correlated with male children (OR = 1.208, CI 95% 0.769–1.352), children of urban areas (OR = 0.594, CI 95% 0.476–1.987) and food shortage (OR = 1.367, CI 95% 0.782–2.543). Underweight status was close relationship with male gender (OR = 1.213, CI 95% 0.821–2.897), incomplete immunization (OR = 1.342, CI 95% 1.041–2.658) and treated water access (OR = 0.689, CI 95% 0.542–2.743).

Conclusion
In this study, prevalence of higher-level malnutrition was estimated due to lower socioeconomic status among major population in the community specifically in rural areas. The government needs to enhance economic opportunities, promote healthcare education, increase nutritional access and generate improving personal hygiene habits through appropriate economic, political and social policy measures. The outcome of these policies will enhance individual economic resources; ensure nutritional safety, increasing maternal schooling and helpful for improving child health.
Cognition and affect in consumer decision making: conceptualization and validation of added constructs in modified instrument
Authors: Sofi Shakeel Ahmad , Mir Faizan Ashraf , Baba Mubashir Majid 
Published: August 2020

AbstractCognition and affect have had stretched history of influencing the buying behaviour of an individual. The change in one of the dimensions leads to some proportionate change in corresponding factor, and a number of research studies have been carried out to ascertain the role of cognition and affect in consumer decision making. But most of the studies lack the evidence of scientific reliability and validity and nature of itemization in previous scales/papers has not been comprehensive as well. In the current endeavour, application of exploratory factor analysis and structure equation modelling has significantly tested the reliability and validity measures needed for impulsive buying scale that would largely facilitate different stake holders. This paper explores the process for how highly reliable and valid indicators of cognition and affect have been developed. The research design employed was a mixture of both exploratory and descriptive approaches that assisted author in classifying factors along with underlying items. Structured questionnaire was employed for the collection of data from the respondents. For validation and development of the modified scale, a set of reliable and scientific tools were employed included. Overall findings revealed that the instrument is vastly consistent and possesses both discriminant and convergent validity. Additionally, other reliability forms are on higher side which sustains the reliability of the scale. The current study will have larger credibility for researchers in the area of organizational behaviour, consumer behaviour and in other interdisciplinary areas.
Revisiting cancer hallmarks: insights from the interplay between oxidative stress and non-coding RNAs
Authors: Zhou Li , Zhang Zhe , Huang Zhao , Nice Edouard , Zou Bingwen , Huang Canhua 
Published: August 2020

AbstractCancer is one of the most common disease worldwide, with complex changes and certain traits which have been described as “The Hallmarks of Cancer.” Despite increasing studies on in-depth investigation of these hallmarks, the molecular mechanisms associated with tumorigenesis have still not yet been fully defined. Recently, accumulating evidence supports the observation that microRNAs and long noncoding RNAs (lncRNAs), two main classes of noncoding RNAs (ncRNAs), regulate most cancer hallmarks through their binding with DNA, RNA or proteins, or encoding small peptides. Reactive oxygen species (ROS), the byproducts generated during metabolic processes, are known to regulate every step of tumorigenesis by acting as second messengers in cancer cells. The disturbance in ROS homeostasis leads to a specific pathological state termed “oxidative stress”, which plays essential roles in regulation of cancer progression. In addition, the interplay between oxidative stress and ncRNAs is found to regulate the expression of multiple genes and the activation of several signaling pathways involved in cancer hallmarks, revealing a potential mechanistic relationship involving ncRNAs, oxidative stress and cancer. In this review, we provide evidence that shows the essential role of ncRNAs and the interplay between oxidative stress and ncRNAs in regulating cancer hallmarks, which may expand our understanding of ncRNAs in the cancer development from the new perspective.
Leisure-Time and Occupational Physical Activity Associates Differently with Epigenetic Aging
Authors: KANKAANPÄÄ ANNA , TOLVANEN ASKO , BOLLEPALLI SAILALITHA , LESKINEN TUIJA , KUJALA URHO M. , KAPRIO JAAKKO , OLLIKAINEN MIINA , SILLANPÄÄ ELINA 
Published: August 2020

ABSTRACT

Purpose
Greater leisure-time physical activity (LTPA) associates with healthier lives, but knowledge regarding occupational physical activity (OPA) is more inconsistent. DNA methylation (DNAm) patterns capture age-related changes in different tissues. We aimed to assess how LTPA and OPA are associated with three DNAm-based epigenetic age estimates, namely, DNAm age, PhenoAge, and GrimAge.

Methods
The participants were young adult (21–25 yr, n = 285) and older (55–74 yr, n = 235) twin pairs, including 16 pairs with documented long-term LTPA discordance. Genome-wide DNAm from blood samples was used to compute DNAm age, PhenoAge, and GrimAge Age acceleration (Acc), which describes the difference between chronological and epigenetic ages. Physical activity was assessed with sport, leisure-time, and work indices based on the Baecke Questionnaire. Genetic and environmental variance components of epigenetic age Acc were estimated by quantitative genetic modeling.

Results
Epigenetic age Acc was highly heritable in young adult and older twin pairs (~60%). Sport index was associated with slower and OPA with faster DNAm GrimAge Acc after adjusting the model for sex. Genetic factors and nonshared environmental factors in common with sport index explained 1.5%–2.7% and 1.9%–3.5%, respectively, of the variation in GrimAge Acc. The corresponding proportions considering OPA were 0.4%–1.8% and 0.7%–1.8%, respectively. However, these proportions were minor (<0.5%) after adjusting the model for smoking status.

Conclusions
LTPA associates with slower and OPA with faster epigenetic aging. However, adjusting the models for smoking status, which may reflect the accumulation of unhealthy lifestyle habits, attenuated the associations.
The Effect of Blood Ketone Concentration and Exercise Intensity on Exogenous Ketone Oxidation Rates in Athletes
Authors: DEARLOVE DAVID J. , HARRISON OLIVIA K. , HODSON LEANNE , JEFFERSON ANDREW , CLARKE KIERAN , COX PETE J. 
Published: August 2020

ABSTRACT

Introduction
Exogenous ketones potentially provide an alternative, energetically advantageous fuel to power exercising skeletal muscle. However, there is limited evidence regarding their relative contribution to energy expenditure during exercise. Furthermore, the effect of blood ketone concentration and exercise intensity on exogenous ketone oxidation rates is unknown.

Methods
Six athletes completed cycling ergometer exercise on three occasions within a single-blind, random-order controlled, crossover design study. Exercise duration was 60 min, consisting of 20-min intervals at 25%, 50%, and 75% maximal power output (W
Max). Participants consumed (i) bitter flavored water (control), (ii) a low-dose β-hydroxybutyrate (βHB) ketone monoester (KME; 252 mg·kg BW−1, “low ketosis”), or (iii) a high-dose βHB KME (752 mg·kg BW−1, “high ketosis”). The KME contained a 13C isotope label, allowing for the determination of whole-body exogenous βHB oxidation rates through sampled respiratory gases.

Results
Despite an approximate doubling of blood βHB concentrations between low- and high-ketosis conditions (~2 mM vs ~4.4 mM), exogenous βHB oxidation rates were similar at rest and throughout exercise. The contribution of exogenous βHB oxidation to energy expenditure peaked during the 25% W
Max exercise intensity but was relatively low (4.46% ± 2.71%). Delta efficiency during cycling exercise was significantly greater in the low-ketosis (25.9% ± 2.1%) versus control condition (24.1% ± 1.9%; P = 0.027).

Conclusions
Regardless of exercise intensity, exogenous βHB oxidation contributes minimally to energy expenditure and is not increased by elevating circulating concentrations greater than ~2 mM. Despite low exogenous βHB oxidation rates, exercise efficiency was significantly improved when blood βHB concentration was raised to ~2 mM.
Constipation Predominant Irritable Bowel Syndrome and Functional Constipation Are Not Discrete Disorders: A Machine Learning Approach
Authors: Ruffle James K. , Tinkler Linda , Emmett Christopher , Ford Alexander C. , Nachev Parashkev , Aziz Qasim , Farmer Adam D. , Yiannakou Yan 
Published: August 2020

INTRODUCTION:
Chronic constipation is classified into 2 main syndromes, irritable bowel syndrome with constipation (IBS-C) and functional constipation (FC), on the assumption that they differ along multiple clinical characteristics and are plausibly of distinct pathophysiology. Our aim was to test this assumption by applying machine learning to a large prospective cohort of comprehensively phenotyped patients with constipation.

METHODS:
Demographics, validated symptom and quality of life questionnaires, clinical examination findings, stool transit, and diagnosis were collected in 768 patients with chronic constipation from a tertiary center. We used machine learning to compare the accuracy of diagnostic models for IBS-C and FC based on single differentiating features such as abdominal pain (a “unisymptomatic” model) vs multiple features encompassing a range of symptoms, examination findings and investigations (a “syndromic” model) to assess the grounds for the syndromic segregation of IBS-C and FC in a statistically formalized way.

RESULTS:
Unisymptomatic models of abdominal pain distinguished between IBS-C and FC cohorts near perfectly (area under the curve 0.97). Syndromic models did not significantly increase diagnostic accuracy (P > 0.15). Furthermore, syndromic models from which abdominal pain was omitted performed at chance-level (area under the curve 0.56). Statistical clustering of clinical characteristics showed no structure relatable to diagnosis, but a syndromic segregation of 18 features differentiating patients by impact of constipation on daily life.

DISCUSSION:
IBS-C and FC differ only about the presence of abdominal pain, arguably a self-fulfilling difference given that abdominal pain inherently distinguishes the 2 in current diagnostic criteria. This suggests that they are not distinct syndromes but a single syndrome varying along one clinical dimension. An alternative syndromic segregation is identified, which needs evaluation in community-based cohorts. These results have implications for patient recruitment into clinical trials, future disease classifications, and management guidelines.
Obesity, Diabetes, Coffee, Tea, and Cannabis Use Alter Risk for Alcohol-Related Cirrhosis in 2 Large Cohorts of High-Risk Drinkers
Authors: Whitfield John B. , Masson Steven , Liangpunsakul Suthat , Mueller Sebastian , Aithal Guruprasad P. , Eyer Florian , Gleeson Dermot , Thompson Andrew , Stickel Felix , Soyka Michael , Muellhaupt Beat , Daly Ann K. , Cordell Heather J. , Foroud Tatiana , Lumeng Lawrence , Pirmohamed Munir , Nalpas Bertrand , Jacquet Jean-Marc , Moirand Romain , Nahon Pierre , Naveau Sylvie , Perney Pascal , Haber Paul S. , Seitz Helmut K. , Day Christopher P. , Mathurin Philippe , Morgan Timothy R. , Seth Devanshi ,   for the GenomALC Consortium
Published: August 2020

INTRODUCTION:
Sustained high alcohol intake is necessary but not sufficient to produce alcohol-related cirrhosis. Identification of risk factors, apart from lifetime alcohol exposure, would assist in discovery of mechanisms and prediction of risk.

METHODS:
We conducted a multicenter case-control study (GenomALC) comparing 1,293 cases (with alcohol-related cirrhosis, 75.6% male) and 754 controls (with equivalent alcohol exposure but no evidence of liver disease, 73.6% male). Information confirming or excluding cirrhosis, and on alcohol intake and other potential risk factors, was obtained from clinical records and by interview. Case-control differences in risk factors discovered in the GenomALC participants were validated using similar data from 407 cases and 6,573 controls from UK Biobank.

RESULTS:
The GenomALC case and control groups reported similar lifetime alcohol intake (1,374 vs 1,412 kg). Cases had a higher prevalence of diabetes (20.5% (262/1,288) vs 6.5% (48/734), P = 2.27 × 10−18) and higher premorbid body mass index (26.37 ± 0.16 kg/m2) than controls (24.44 ± 0.18 kg/m2, P = 5.77 × 10−15). Controls were significantly more likely to have been wine drinkers, coffee drinkers, smokers, and cannabis users than cases. Cases reported a higher proportion of parents who died of liver disease than controls (odds ratio 2.25 95% confidence interval 1.55–3.26). Data from UK Biobank confirmed these findings for diabetes, body mass index, proportion of alcohol as wine, and coffee consumption.

DISCUSSION:
If these relationships are causal, measures such as weight loss, intensive treatment of diabetes or prediabetic states, and coffee consumption should reduce the risk of alcohol-related cirrhosis.
MENAGIH HAK BERAGAMA MUSLIM AHMADIYAH DAN SYIAH SAMPANG DALAM PERSPEKTIF NALAR MAQÂṢIDÎ
Authors: Yaqin Nasrullah Ainul 
Published: August 2020

This article responds to the issue of human rights (HAM) from the perspective of maqâṣidî’s reasoning (maqâṣid asy-syarî‘ah). Considering that human rights violations have always been being a boomerang in the life of Indonesian society. It could be seen like what happened to the Shia people in Sampang whose right have been lost to enjoy their life in peace and worship their religious rights freely and safely in their homeland. A similar case has been experienced by several Ahmadiyah congregations. In this case, the writer elaborates the maqâṣid concept which has been continued its development along the time. According to ‘Izzuddin bin ‘Abd as-Salâm, the purpose of Islamic law is to create benefit and reject the damage for human being, both in this world and in the hereafter. The highest benefit of Islamic law is to maintain and keep the religion (ḥifẓ ad-dîn), soul (ḥifẓ an-nafs), reason (ḥifẓ al-‘aql), descent (ḥifẓ an-nasl), and property (ḥifẓ al-mâl) . These five things are known as aḍ-ḍarûriyyah al-khamsah (the five primary). Later, some maqâṣidî scholars (such as Ibn ‘Âsyûr, ‘Allâl al-Fâsî, Jamâluddîn ‘Aṭiyyah, and Yusûf al-Qarâḍâwî) developed the scope of maqâṣid asy-syarî‘ah. So that, it is not restricted to the five primary matters only, but also to several other primary matters, such as justice, freedom, equality and human rights. On the other side, Muḥammad az-Zuḥailî views that the concept of aḍ-ḍarûriyyah al-khamsah is the basis of human rights itself. Because, ḥifẓ ad-dîn, ḥifẓ an-nafs, ḥifẓ al-‘aql, ḥifẓ an-nasl, and ḥifẓ al-mâl do not only mean to maintain, but also include the meaning of rights, namely: the right to religion, the right to life, the right to think and freedom of thought, family rights, and property rights.
Identification of Waste Flow in Semarang City Through Community-Based Waste Management
Authors: Ramadan Bimastyaji Surya , Fauziyah Fita , Budihardjo Mochamad Arief , Syafrudin Syafrudin 
Published: August 2020

Community-based waste management is a form of waste management recommended by the government because it is very effective in creating a zero-waste society. In Indonesia, community-based waste management can be realized through the development of waste banks at the neighbourhoodlevels. In 2019, the City of Semarang has 48 units of active waste banks. Waste that is managed by the wastebank is plastic, paper and metal. However, there is organic waste which is also managed by several wastebanks. The purpose of this study is to identify and analyze the flow of waste through community-basedwaste management, in this case of the waste bank, in the city of Semarang. The method used is in-depth surveys and interviews related to the waste bank management system in the city of Semarang. The results showed that the activity of the existing garbagebank was able to reduce waste in the city of Semarang by 0.07% of the total garbage generation in the city of Semarang. This research can be used as a reference for developing better community-based waste management strategies, especially in Semarang City
The Use of Nano-Bio Coagulant from Rice Snail (Pila Ampullacea) Shells to Reduce COD, Turbidity, and TSS of Pharmaceutical Industry Wastewater
Authors: Hadiwidodo Mochtar , Ainurrofiq Mohammad Naffah , Purwono Purwono , Oktiawan Wiharyanto 
Published: August 2020

Salah satu industry farmasi di Semarang, Jawa Tengah menggunakan koagulan Poly Alumunium Chloride (PAC) untuk mengolah limbah cair. Penggunaan PAC atas dasar kelayakan biaya dan efektivitas pengolahan. Apabila ditinjau dari aspek lingkungan, penggunaan koagulan sintetik dalam jumlah besar akan menimbulkan limbah lumpur yang sulit didegradasi, dan mampu mengubah tingkat keasaman air dan tanah disekitarnya, sehingga berdampak buruk bagi lingkungan. Pada penelitian ini kitosan digunakan sebagai nano bio koagulan untuk mengolah limbah cair industri farmasi. Variasi dosis nano bio koagulan dan kecepatan pengadukan dilakukan untuk mengevaluasi efisiensi penyisihan parameter Chemical Oxygen Demand (COD), kekeruhan, dan Total Suspended Solid (TSS), limbah. Nani bio koagulan dibuat dari Cangkang keong Sawah (Pila Ampullacea) dan ukuran biokoagulan dibuat skala nano partikel dengan harapan mampu meningkatkan efektifitas penyisihan. Metode persiapan berupa deproteinasi, demineralisasi dan deasetilasi. Proses nano partikel menggunakan alat HEM, ukuran partikel diuji menggunakan SEM dan uji gugus fungsi menggunakan FTIR. Hasil penelitian menunjukkan bahwa kitosan memiliki warna coklat abu-abu, ukuran partikel serbuk nano, kadar air 5,34 %, kadar abu 1,14 % dan derajat deasetil 25,27 %. Efisiensi penyisihan TSS yang tinggi sebesar 55,19 %, kekeruhan 64,73 % dan COD 55,63 %. Dosis yang optimum sebesar 200 mg/L dengan kecepatan pengadukan cepat 150 rpm. Nano biokoagulan kitosan paling efektif untuk menyisihkan kekeruhan dibandingkan dengan COD dan TSS limbah cair indutri farmasi.
Carbon and Microbial Dynamics in Soil on Biochar Compost Plus Treatment
Authors: Purbalisa Wahyu , Zulaehah Ina , Paputri Dolty Melyga W. , Wahyuni Sri 
Published: August 2020

Carbon and microbes in the soil fluctuated from time to time due to various things. This study aims to determine the dynamics of carbon and microbes in the soil in the treatment of biochar-compost. In addition to the use of biochar-compost, this research also uses nano biochar and enrichment with microbial consortia. The study was conducted at the screen house using a complete randomized design with three replications with following treatments: control / without organic fertilizer (P0), compost (P1), biochar-compost 1: 4 (P2), nano-biochar-compost 1: 4 (P3 ), biochar-compost + microbial consortia (P4), compost + microbial consortia (P5) and biochar-compost + microbial consortia (P6) with a dose of 2.5 tons/ha respectively. Biochar comes from corncobs. Compost biochar plus application was made before planting. Parameters observed were soil carbon (C-organic), soil acidity (pH) at 7 DAA, 37 DAA and after harvest, and the total soil microbial population at 2 DAA and after harvest. Soil carbon was measured using Walkey and Black method measured by spectrophotometer, soil pH using a soil: water ratio = 1: 5 and measured by a pH meter, the total microbial population using Total Plate Counting (TPC) method. The results showed carbon and soil microbial populations decreased over time, except for microbial communities in a single compost treatment.