bluuebunny/crossref_metadata_2025_split huggingface dataset:
Application of Ginkgo biloba L. extract at early stage of tumor development helps cyclophosphamide inhibit the growth of tumor cells
Authors: Chen Dawei , Yang Jinghui , Meng Fanzhu , Beseler Cheryl L. , Li Hao , Liu Xiaomei , Guo Yinpei , Qin Lili , Zuo Ruyue , Ren Shuping 
Published: December 2020

Abstract
Background: There is inconsistency in the application of antioxidants in tumor treatment. This study explored whether the application of an antioxidant at the early stage of tumor transplantation enhances efficacy of a chemotherapeutic agent in inhibiting tumor cell growth. Methods: EMT-6 cells were injected into 48 ICR mice. 24h later, cyclophosphamide (CTX) and Ginkgo biloba L. extract (GBE) were administered to mice separately and in combination. Apoptotic markers and related signaling pathways were measured. Tumor weights were compared and survival analysis was used to investigate latency periods in the three treatment groups compared to a PBS control group. Results: The mice administered GBE and CTX had significantly lower tumor weights compared with those administered PBS (p=0.01), however, the mice administered CTX did not have significantly lower tumor weight compared with those administered PBS (p=0.19). The expression of NF-κB, IκB-ɑ,, and the phosphorylation of NF-κB, IκB-ɑ, were all significantly decreased in the tumors from GBE+CTX (p<0.05). Moreover, the ratio of Bax/Bcl-2 was significantly higher for the tumors of mice administered GBE+CTX than that of mice administered GBE, CTX, or PBS (p<0.05), the expression of FADD, Caspase-8, and Cyto-C was significantly increased in the tumors of mice administered GBE+CTX compared with those administered PBS or GBE (p<0.05). Conclusions: Application of an antioxidant at an early stage of tumor transplantation could help chemotherapeutic agents inhibit the growth of tumor cells. GBE+CTX treated mice showed a longer latency period to tumor development and markers that consistently indicated greater apoptotic activity and reduced tumor promotion.
Feasibility of a silicon thin film transistor-based aptamer sensor for COVID-19 detection
Authors: Farrow Thomas , Laumier Siriny , Hall Steve , Sandall Ian , Zalinge Harm van 
Published: December 2020

Abstract
Since the beginning of the coronavirus disease 2019 (COVID-19) in December 2019 and the current lack to date of specific drugs or vaccinations to cope with the disease, it has become apparent that the surest way of dealing with this is through early diagnosis and management. Current testing has shown to be unable to rapidly and accurately provide the results required to restrict the spread. Here we report feasibility for the use of an intrinsic silicon thin film transistor functionalised with aptamers designed to attach to the spike protein of COVID-19. It is shown that a linear response can be obtained in a concentration range of 1 pM to 1 nM.
Co odróżnia ekotoksykologię od ekologii?
Authors: Sosak-Świderska Bożena 
Published: December 2020

Ecotoxicology is concerned with the toxic effects of chemical and physical agents on living organisms, especially on populations and communities within defined ecosystems; it includes the transfer pathways of those agents and their interactions with the environment. Rene Truhaut, a French toxicologist coined the term ecotoxicology - a new branch of toxicology which defined as the study of the adverse effects of xenobiotics (foreign substances). Both toxicology and ecology are well established. Ecological studies do not typically deal with the organism itself but stress the relation of organisms to their environments. On the contrary toxicological effects occur at many scales: at the cellular or tissue level and enzymes are induced. The most difficult question it seems in ecotoxicology is the choice of the endpoint. Environmentally appealing endpoints such as NOEC (no observable effects concentrations) are difficult to justify Ecotoxicology implies the interfacing of the fields of ecology and environmental toxicology at the population level, there are mortality and selection; at the community level, there is the replacement of species. Most ecotoxicological research to present has used previously existing methods that were expanded into this new field of research, rather than developing new or integrated practices. Research into the transport and chemical transformation of substances has become the field of environmental chemistry, which makes use of analytical techniques (for example QSAR). Ecotoxicology or environmental toxicology and ecology have distinct research programs that identify different problems and that take different things for granted. In addition are there the differences in tools, methods, theories, and concepts, scientists in each of the two disciplines view them as two different fields. Ecotoxicology is a multidisciplinary science and still has not been able to establish itself.
Recent Progress in Prediction Systems for Drug-induced Liver Injury Using In vitro Cell Culture
Authors: Ozawa Shogo , Miura Toshitaka , Terashima Jun , Habano Wataru , Ishida Seiichi 
Published: December 2020

Background:In order to avoid drug-induced liver injury (DILI), in vitro assays, which enable the assessment of both metabolic activation and immune reaction processes that ultimately result in DILI, are needed.Objective:In this study, recent progress in the application of in vitro assays using cell culture systems is reviewed for potential DILI-causing drugs/xenobiotics and a mechanistic study on DILI, as well as on the limitations of in vitro cell culture systems for DILI research, was carried out.Methods:Information related to DILI was collected through a literature search of the PubMed database.Results:The initial biological event for the onset of DILI is the formation of cellular protein adducts after drugs have been metabolically activated by drug metabolizing enzymes. The damaged peptides derived from protein adducts lead to the activation of CD4+helper T lymphocytes and recognition by CD8+cytotoxic T lymphocytes, which destroy hepatocytes through immunological reactions. Because DILI is a major cause of drug attrition and drug withdrawal, numerous in vitro systems consisting of hepatocytes and immune/inflammatory cells or spheroids of human primary hepatocytes containing non-parenchymal cells have been developed. These cellular-based systems have identified DILI-inducing drugs, with approximately 50% sensitivity and 90% specificity.Conclusion:Different co-culture systems consisting of human hepatocyte-derived cells and other immune/inflammatory cells have enabled the identification of DILI-causing drugs and of the actual mechanisms of action.
Micronutrient Biofortification in Rice through New Breeding Techniques (NBTs): Bangladesh Perspective
Authors: Sarkar Bishajit , Akter Fayza , Johora Fatema Tuz , Ullah Md. Asad , Shohael Abdullah Mohammad 
Published: December 2020

Background:
Micronutrient deficiencies are serious health issues in developing countries of Asia and Africa,
where millions of people are suffering from inadequate micronutrient intake. In Bangladesh, micronutrient deficiencies
are found severe due to low income, food habits, and rice-based staple food consumption, (rice has an insufficiency of
different types of vitamins and minerals). To lessen micronutrient malnutrition, supplementation has been employed but
has not yet reached the goal. Agronomic and genetic biofortification has the potential to address micronutrient
deficiencies. Biofortification in Rice grain is a convenient and affordable way to supply the desired micronutrients. The
development of micronutrient-rich popular rice cultivars through conventional breeding is currently being harnessed for
the limitation of natural resources of the related donor rice cultivars containing the required amount of micronutrients. To
overcome these hurdles of conventional breeding, genetic engineering and genome editing have emerged as promising
tools of micronutrient biofortification in rice.

Methods:
Identify the needs and explore the potential strategies by the search for relevant literature known to the authors
was carried out to complete this review.

Results:
Highlighted here the sources, functions, and requirements of iron, zinc, vitamin A, vitamin B1, vitamin B9, and
betanin in rice and their biofortification through conventional breeding, genetic engineering, and genome editing including
their promises and hindrances.

Conclusion:
New breeding techniques are timely alternatives for developing nutrient-rich rice cultivars to eliminate
hidden hunger and poverty in Bangladesh.
Meningkatkan literasi informasi penulisan karya ilmiah mahasiswa melalui pelatihan zotero
Authors: Anjali Muhammad Erdiansyah Cholid , Istiqomah Zeni 
Published: December 2020

Introduction. The purpose of this study is to examine a zotero training conducted by Universitas Muhammadiyah Yogyakarta (UMY) Library, information literacy skills, and the role of the zotero training on the literacy skills in students’ academic writing.
Data Collection Method. The data collection method used was a descriptive qualitative with observation and interviews. The participants were students study of Master of Government Affairs and Administration program at UMY.
Data Analysis. Data analysis was performed by validating the collected data which were then categorized based on the theory.
Results and Discussions. The results showed that students received the zotero training well. The material provided in the training including the explanation of the Zotero concept, installation of the Zotero application, and utilization of the Zotero application were well understood.
Conclusion. The ability of information literacy of students of Master of Government Affairs and Administration, which was initially limited, increases by the Zotero training involvement. These capabilities include how to identify information needs, access information, evaluate information, and use information effectively.
Pelestarian pengetahuan lokal melalui transfer pengetahuan di Rurukan Adat “Nabawadatala” Sumedang
Authors: Waty Windi Widia , Yusup Pawit M. , Yanto Andri 
Published: December 2020

Introduction. The research aims to examine knowledge transfer by a local community Rurukan Adat "Nabawadatala" in preserving local knowledge ion Citengah village – Sumedang, West Java.
Data Collection Method. This research used qualitative approach with a case study method.
Data Analysis. Qualitative data analysis was conducted iteratively and continuously until reach the data saturation. The three steps of data analysis were conducted, including data reduction, data display, and conclusion drawing/verification.
Results and Discussion. Pattern transfer occurred in the study includes three processes, namely the preparation of sustainable work plan; the concept of learning and knowledge sharing; and the creation of knowledge to form a tourist area-based education.
Conclusion. A cultural centre established by the Citengah village community leaders to support knowledge transfer is very useful, not only for people inside of the community, but also for people around the community.
Identifikasi kecakapan inovasi lembaga riset di Indonesia berbasis dokumen
Authors: Yaman Aris , Sartono Bagus , Sholeh Agus M. 
Published: December 2020

Introduction. Duplication in inventions produced by research institutions in Indonesia becomes an issue. It is important to map the specialization of the invention in research institutions. This study examines the mapping of the innovation in research institutions in Indonesia.
Data Collection Method. This study uses a patent-based technology document analysis method to map the potential of technology. The data used is patent data registered in the Direktorat Jenderal Kekayaan Intelektual (DJKI) database.
Data Analysis. Metadata analysis was conducted by using the K-Means Klastering method with R software.
Results and Discussions. The findings in the pre-analysis show that when the independent variable involved in the model are very large, the Localized feature selection method can effectively select variables without losing much information. There are 5 dominant technology groups that can be produced by research institutions in Indonesia, namely 1) Technology related to the development of measurement and testing instrument technology; 2) Technologies related to food and food ingredients; and 3) microstructural test equipment / detectors; 4) radar technology; 5) Technology in agriculture.
Conclusion. The findings show that there are still overlapping inventions by several research institutions in the same technology cluster. K-means clustering with LFSBSS pre analysis has a clear performance in the technology cluster space.
Layanan referensi virtual perpustakaan Universitas Leiden melalui perpustakaan KITLV-Jakarta
Authors: Shaura Rizkiana Karmelia , Salim Tamara Adriani , Kurniawan Hendra 
Published: December 2020

Introduction. This study discusses Leiden University Library reference services through the KITLV-Jakarta. Since KITLV was acquired by the Leiden University Library, the KITLV-Jakarta Library which was a KITLV-Dutch representative, is now a representative of the Leiden University Library in Jakarta.
Data Collection Method. This study used a qualitative approach with descriptive research involving observations and interviews as well as primary document data sources.
Data Analysis. The data was analyzed by involving the process of data reduction, interpretation, and conclusion.
Results and Discussions. The results of the study indicate that reference services, particularly for reference collections found in the Leiden University Library cannot be accessed directly through the KITLV-Jakarta Library. Only KITLV librarians can request the reference service from Leiden University librarians.
Conclusion. Librarians at the KITLV-Jakarta Library do not have the same access as librarians at the Leiden University Library, even though the KITLV-Jakarta Library is a representative of the Leiden University Library in Jakarta.
Pemanfaatan portal basis data daring dalam validasi nama ilmiah jenis dan suku tumbuhan
Authors: Damayanto I Putu Gede P. , Fastanti Fandri S. , Dalimunthe Syadwina H. 
Published: December 2020

Introduction. The study of the validation of scientific name validation at the species and family level is not widely known. This study aimed to provide information about the process of scientific name validation at the species and family level.
Data Collection Method. Data were collected from online portal databases regarding the scientific name of plant species and family. Data was analyzed in a descriptive-narrative manner.
Results and Discussions. Validation of the scientific name of species and family of plants can be conducted by searching the latest publications and using the online database portals i.e. IPNI, POWO, Tropicos, The Plant List, and GBIF. IPNI and The Plant List provide more information about species names compared to others. POWO and GBIF provide the status of the plant names and complemented with photographs of the plants, specimens, and distribution maps. Tropicos provides names of families according to the latest APG IV classification.
Conclusions. Validation through the online portal database is the fastest way, however, validation through the publication of the latest plant taxonomy revision publications is the most recommended. Validation can be initiated by using the online database portals and then confirmed by tracking the latest revised plant taxonomy publications.
ANTIMICROBIAL PROPERTIES OF SEVEN BROWN ALGAE HARVESTED FROM THE COAST OF SIDI BOUZID (EL JADIDA-MOROCCO)
Authors: SAMRI NAZHA , HSAINE LAILA , ELKAFHI SOUKAINA , KHLIFI SAMIRA , ETAHIRI SAMIRA 
Published: December 2020

Objective: This work aims at the screening of the antimicrobial activity of the seven brown marine algae of theCoast of Sidi Bouzid (El Jadida-Morocco).
Methods: The aim of this study was to evaluate the antimicrobial activity of seven brown marine algae against three Gram-positive bacteria (Staphylococcus epidermidis, Staphylococcus aureus and Streptococcus pyogenes). Three Gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumonia) and two fungi (Aspergillus Niger and Candida tropicalis). Thus, 35 algal extracts were prepared with five organic solvents methanol/water, methanol, dichloromethane/methanol, dichloromethane and ethyl acetate. The antibacterial activity was evaluated through the disk diffusion method.
Results: Data revealed that the Staphylococcus aureus bacteria was the most sensitive pathogen by showing the highest zone of inhibitions of 20 mm with the lowest Minimum Inhibitory Concentration (MIC) of 2 μgmL⁻¹ methanol/water extract of Cystoseira tamariscifolia. Whereas, antifungal activity, the highest zone of inhibitions of 21 mm and 22 mm with the lowest Minimum Inhibitory Concentration (MIC) of 5 μgmL-¹ was respectively shown in the methanol/water extract of Laminaria ochroleuca against Candida tropicalis and in the dichloromethanolic extract of Sargassum vulgare against Aspergillus niger.
Conclusion: The results indicate that these algal extracts can further be analyzed and purified for relevant antibacterial and antifungal compounds which can be used in therapeutics and other applications.
ESCIN MITIGATES HYPOXIA MIMICKING NCI-H23 CELLS THROUGH MODULATION OF MMPs, HIF-1α AND HIF-2α
Authors: CHERMAKANI PANNEER SELVAM , SUDHANDIRAN GANAPASAM 
Published: December 2020

Objective: The objective of the study is to investigate the effect of escin in hypoxia mimicked NCI-H23 cells through the modulation of matrix metalloproteinases (MMPs) 2 and 9.
Methods: In escin-treated NCI-H23 cells, adhesion, migration, and invasion were detected by the adhesion, wound healing, and Boyden chamber assays, respectively. The activation of proteinases was detected using zymography assay. The expressions of HIF-1α and HIF-2α were evaluated by immunoblot.
Results: In the present study, it was observed that escin suppressed chemically induced hypoxia condition and stimulated adhesion, migration, and invasion of NCI-H23 cells. Gelatin zymography assay showed that escin inhibited CoCl2 induced MMPs-2 and 9 activations in NCI-H23 cells. Furthermore, immunoblot analysis revealed that escin treatment decreased the expression of both HIF-1 and 2α in a dose-dependent manner under CoCl2 induced hypoxia condition.
Conclusion: Taken together, these results indicate that escin inhibits HIFs-α mediated MMPs-2 and 9 expressions, resulting in suppression of lung cancer cell invasion that is induced by chemically induced hypoxia condition. Escin is a potential therapeutic agent for clinical use in preventing the invasion of human malignant lung tumors.
PATENT MEDICINE VENDORS’ PREPAREDNESS AND RESPONSE FOR THE PREVENTION AND CONTROL OF COVID-19 INFECTION IN A NORTH-CENTRAL STATE IN NIGERIA
Authors: ILESANMI OLAYINKA S. , AFOLABI AANUOLUWAPO A. , FAKAYODE OLUWATOSIN E. 
Published: December 2020

Objective: This report aimed to determine the immediate effect of the IPC training on the knowledge and role of patent medicine vendors (PMV) in the COVID-19 outbreak response in Nigeria.
Methods: We conducted a quasi-experimental study before and after the IPC training among 1076 PMV in Ilorin, Kwara state, Nigeria. Data analysis was done using SPSS version 23. Eight questions were asked, for which each was assigned a score of “1”. Participants who had scored<50% were categorized as “Fail”, while those with scores ≥50% were categorized as “Pass” in both the pre and post-tests. The Chi-square test was used to test for the association between sociodemographic characteristics and performance of respondents in the pre-and post-IPC knowledge results, and paired t-test to test for significant differences between the pre and post-tests mean scores. The level of significance was set at p<0.05 for all statistical tests.
Results: The mean age of the 1076 respondents was 37.06±10.96, 463(43%) passed the pre-test, while 617 (57.3%) passed the post-test. The mean pre-test IPC training score was 3.1310±1.5631, while the mean post-test IPC training score was 3.7351±1.6028 (t=-11.337, p=<0.001). In all, 617(57.3%) scored higher in posttest. Overall, 560 (92.41%) identified community sensitization and health education on COVID-19 preventive measures as roles of PMV.
Conclusion: Regular trainings on COVID-19 preventive practices among PMV should be conducted. PMV should be empowered to sensitize and educate their clients on the prevention of COVID-19.
UNIDADE DE SAÚDE MÃE CURITIBANA: SATISFAÇÃO DAS USUÁRIAS. HEALTH CENTER “MÃE CURITIBANA”: SATISFACTION OF USERS.
Authors: Gonçalves Cláudia Giglio de Oliveira , Soares Vânia Muniz Nequer , Berberian Ana Paula 
Published: December 2020

Objetivo: analisar o perfil, conhecimento e a satisfação dos usuários da Unidade de Saúde Mãe Curitibana/SUS. Métodos: estudo de caso com usuárias da Unidade Básica de Saúde Mãe Curitibana. Foram aplicados questionários sobre perfil social, utilização e percepção dos serviços do SUS e controle social. Resultados: participaram 500 mulheres, com idade média de 32,9 anos, a maioria, trabalhadoras com elevado nível de escolaridade e oriundas de bairros centrais. Referiram satisfação quanto ao tempo para o atendimento, aos esclarecimentos de dúvidas, e como necessidades apontaram a contratação de mais médicos, as usuárias pouco utilizam os mecanismos de controle social. Conclusões: Evidenciaram-se desigualdades de acesso a unidade estudada, que podem ser equacionadas com a regionalização dos serviços de referencia a mulher em distritos com piores indicadores sociais. O estudo aponta a necessidade de divulgação dos princípios e diretrizes do SUS para maior participação da população na identificação de problemas e de soluções compartilhadas.
DESEMPENHO MOTOR DE ESCOLARES QUILOMBOLAS ENTRE 8 E 10 ANOS
Authors: Fernandes Juliêta Lopes , Santiago Maria Luci Esteves , Lima Andrea Conceição Gomes , Nascimento Edirlane Soares do , Moura Thais Norberta Bezerra de 
Published: December 2020

O desenvolvimento motor é uma sequência de etapas evolutivas que ocorre de acordo com a faixa etária, determinado por meio dos requisitos de interação da criança com a tarefa, individualidade biológica e condições do ambiente. O objetivo deste estudo é analisar o desempenho motor de escolares quilombolas entre 8 anos a 10 anos de idade. Trata-se de uma pesquisa quantitativa, do tipo transversal, com amostra constituída por 52 escolares. O instrumento de coleta de dados utilizado foi o protocolo de testes da Escala de Desenvolvimento Motor (EDM) de Rosa Neto. A seleção da amostra foi do tipo intencional. Apresentaram classificação normal médio as variáveis coordenação motora fina, com Quociente Motor (QM) (QM1 = 100,47), motricidade global (QM2 = 100,42) e equilíbrio (QM3 = 98,07). Já a variável esquema corporal teve a classificação normal baixo (QM4 = 82,06). As menores classificações foram nas áreas de organização espacial (QM5 = 73,3) com classificação inferior, e a organização temporal, muito inferior (QM6 = 69,0). Na classificação geral dos escolares, 94% (n = 49) obtiveram níveis de desenvolvimento motor normal (médio e baixo). Um percentual de 4% (n = 2) foi classificado como inferior e 2% (n = 1), muito inferior. Conclui-se que o desenvolvimento motor da maioria dos escolares quilombolas encontra-se dentro dos parâmetros da normalidade.
Palavras-chave: Desenvolvimento infantil. Desempenho psicomotor. Estudantes.