bluuebunny/crossref_metadata_2025_split huggingface dataset:
Integrative taxonomy reveals three new taxa within the Tylototriton asperrimus complex (Caudata, Salamandridae) from Vietnam
Authors: Bernardes Marta , Le Minh Duc , Nguyen Truong Quang , Pham Cuong The , Pham Anh Van , Nguyen Tao Thien , Rödder Dennis , Bonkowski Michael , Ziegler Thomas 
Published: May 2020

The Tylototriton asperrimus complex from northern Vietnam is reviewed based on morphological comparisons and analysis of the mitochondrial marker NADH dehydrogenase subunit 2 (ND2). Based on molecular divergences, which were revealed to be higher than in other congeners, in concert with morphological differences, two new species and one subspecies are described herein: Tylototriton pasmansisp. nov. differs from T. asperrimussensu stricto by 3.2 to 3.6 % genetic divergence and a combination of distinct morphological characters, such as head slightly longer than wide, distinct mid-dorsal ridge, relatively wide distance between the eyes, tips of fingers reaching the eye when foreleg is laid forward, labial and gular folds present, central belly skin with tubercles shaped like transverse wrinkles and distinct, pointy to round rib nodules. The population of T. pasmansisp. nov. consists of two subclades, the nominotypic one occurring on the eastern side of the Da River (or Black River, including Hoa Binh and Phu Tho provinces), and another occurring on the western side (including Son La and Thanh Hoa provinces). These two subclades differ by 2.5 to 3.1 % genetic divergence and distinct morphological characters. The western subclade is herein described as Tylototriton pasmansi obstissp. nov., which differs from the nominotypic form by a wider head, longer and narrower snout, shorter femur length, and an overall less granulose skin, without an increased concentration of warts on the body sides.
A second new species, Tylototriton sparreboomisp. nov. is described from Lai Chau Province. It differs from T. asperrimussensu stricto by 4.1 to 4.2 % and from Tylototriton pasmansisp. nov. by 3.6 to 4.5 % genetic divergences as well as by a combination of distinct morphological characters, such as head longer than wide, tips of fingers reaching nostril when foreleg adpressed along head, rib nodules distinct, round and relatively enlarged, and wide distance between the eyes.
Expression Efficiency of Multiple Il9 Reporter Alleles Is Determined by Cell Lineage
Authors: Kharwadkar Rakshin , Ulrich Benjamin J , Abdul Qayum Amina , Koh Byunghee , Licona-Limón Paula , Flavell Richard A , Kaplan Mark H 
Published: May 2020

Abstract
Generation of allelic gene reporter mice has provided a powerful tool to study gene function in vivo. In conjunction with imaging technologies, reporter mouse models facilitate studies of cell lineage tracing, live cell imaging, and gene expression in the context of diseases. Although there are several advantages to using reporter mice, caution is important to ensure the fidelity of the reporter protein representing the gene of interest. In this study, we compared the efficiency of two Il9 reporter strains Il9citrine and Il9GFP in representing IL-9-producing CD4+ TH9 cells. Although both alleles show high specificity in IL-9–expressing populations, we observed that the Il9GFP allele visualized a much larger proportion of the IL-9–producing cells in culture than the Il9citrine reporter allele. In defining the mechanistic basis for these differences, chromatin immunoprecipitation and chromatin accessibility assay showed that the Il9citrine allele was transcriptionally less active in TH9 cells compared with the wild-type allele. The Il9citrine allele also only captured a fraction of IL-9–expressing bone marrow–derived mast cells. In contrast, the Il9citrine reporter detected Il9 expression in type 2 innate lymphoid cells at a greater percentage than could be identified by IL-9 intracellular cytokine staining. Taken together, our findings demonstrate that the accuracy of IL-9 reporter mouse models may vary with the cell type being examined. These studies demonstrate the importance of choosing appropriate reporter mouse models that are optimal for detecting the cell type of interest as well as the accuracy of conclusions.
Stability of rotation relations in
-algebras
Authors: Hua Jiajie , Wang Qingyun 
Published: May 2020

AbstractLet
$\Theta =(\theta _{j,k})_{3\times 3}$
be a nondegenerate real skew-symmetric

$3\times 3$
matrix, where

$\theta _{j,k}\in [0,1).$
For any

$\varepsilon>0$
, we prove that there exists

$\delta>0$
satisfying the following: if

$v_1,v_2,v_3$
are three unitaries in any unital simple separable

$C^*$
-algebra A with tracial rank at most one, such that

$$\begin{align*}\|v_kv_j-e^{2\pi i \theta_{j,k}}v_jv_k\|<\delta \,\,\,\, \mbox{and}\,\,\,\, \frac{1}{2\pi i}\tau(\log_{\theta}(v_kv_jv_k^*v_j^*))=\theta_{j,k}\end{align*}$$
for all

$\tau \in T(A)$
and

$j,k=1,2,3,$
where

$\log _{\theta }$
is a continuous branch of logarithm (see Definition 4.13) for some real number

$\theta \in [0, 1)$
, then there exists a triple of unitaries

$\tilde {v}_1,\tilde {v}_2,\tilde {v}_3\in A$
such that

$$\begin{align*}\tilde{v}_k\tilde{v}_j=e^{2\pi i\theta_{j,k} }\tilde{v}_j\tilde{v}_k\,\,\,\,\mbox{and}\,\,\,\,\|\tilde{v}_j-v_j\|<\varepsilon,\,\,j,k=1,2,3.\end{align*}$$
The same conclusion holds if

$\Theta $
is rational or nondegenerate and A is a nuclear purely infinite simple

$C^*$
-algebra (where the trace condition is vacuous).
If

$\Theta $
is degenerate and A has tracial rank at most one or is nuclear purely infinite simple, we provide some additional injectivity conditions to get the above conclusion.
4. Las gemas de la organización innovadora: cómo manejar el conocimiento y el aprendizaje
Authors: Hamel de Rodríguez (UPEL-IPB) Yolinel , Pérez de Maldonado  (UPEL-IPB) Isabel 
Published: May 2020

El presente artículo se inscribe en la modalidad documental y tiene como objetivo de reconocer las potencialidades de la Teoría General del Conocimiento y la Teoría de la Acción para potenciar las organizaciones innovadoras, cuyas particularidades las obligan a ser vanguardistas en el uso de tecnologías y a reconocer que el capital humano le aporta valor agregado cuando se le permite participar del entretejido organizacional. A partir de la revisión teórica referencial, se plantea la necesidad de generar una espiral del conocimiento, involucrando: al conocimiento tácito, que nace de lo aprendido en la cotidianidad y de las corazonadas; al conocimiento explícito, que se expresa a través del lenguaje formal en normas de procedimientos, manuales y reglamentos; a la teoría explícita, que se predica y a la teoría-en-uso, que se practica. Se concluye que las organizaciones deben habilitar espacios armónicos que faciliten la exteriorización tanto de las capacidades, habilidades y destrezas individuales como de los supuestos, las inquietudes, las incertidumbres y hasta los descontentos.
Effectiveness of the Use of Indigenous Language in Disseminating Climate Change Information for Sustainable Environmental Development in Nigeria
Authors: Olatumile Adekunle , Tunde-Awe Bola 
Published: May 2020

Climate change has undoubtedly brought with it many seemingly intractable challenges and is already having significant impacts in Nigeria, and these impacts are expected to increase in the future. Rural dwellers environmental behaviour is not all that positive, because the language used in disseminating environmental issues, is mostly English and not the indigenous language. Information disseminated in English Language in most cases is not easily comprehended, because most of the rural dwellers are not literate in it. The instrument used is a self-constructed test. The study adopted the quasi experimental method. Purposeful sample was used to select two groups, with twenty people in each group. The two groups were taught the causes and effects of climate change, one group was taught using English Language and the other group using Yoruba. A test was administered to each group to determine their level of comprehension. Data were analysed using mean and standard deviation. Yoruba group are more in-depth in their knowledge of climate change than English group. The use of mother tongue is germane in the dissemination of information regarding environmental sustainability, however climate change messages will be most effective if there is mix of English Language and Yoruba Language as there is no segment of the society that will be left out. DOI: https://doi.org/10.5281/zenodo.3376478
Rekenen en rekenschap
Authors: Helwig Petra 
Published: May 2020

Rekenen en rekenschap – Algoritmes en de Archiefwet
Binnen de overheid neemt semigeautomatiseerde besluitvorming toe. Parallel daaraan is er steeds meer aandacht voor eisen aan onderliggende algoritmes. Een daarvan is dat algoritmes die de overheid gebruikt bij (deels) geautomatiseerde besluitvorming, transparant en uitlegbaar horen te zijn. Dat past bij een transparante overheid en is ook het recht van individuele burgers voor wie een besluit potentieel rechtsgevolgen heeft. Een gemeente zal een betrokkene bijvoorbeeld moeten informeren waarom hij of zij wordt ‘geprofileerd’ als mogelijke uitkeringsfraudeur. Bij het transparant maken van algoritmes kan niet altijd worden volstaan met een beroep op de kennis van direct betrokkenen, zoals programmeurs. Dit artikel betoogt dat daarom ‘iets’ van gebruikte algoritmes opgenomen zal moeten worden in het archief van de organisatie en moeten worden beheerd in lijn met de Archiefwet. Voor wat dat dan concreet betekent, worden enkele richtingen verkend maar er is nog veel uit te zoeken. De Inspectie Overheidsinformatie en Erfgoed ziet hier, als toezichthouder op de Archiefwet, ook voor zichzelf een rol en zal de ontwikkelingen nader volgen.
Tussen recht en datawetenschap
Authors: Stoepker Giel , Stoepker Ivo 
Published: May 2020

Tussen recht en datawetenschap – Toezicht door voorspellende algoritmische modellen
Datawetenschap is voor het recht van groot belang. De daaruit voortvloeiende voorspellende algoritmische modellen kunnen worden gebruikt om toezicht uit te oefenen, bijvoorbeeld of geen uitkeringsfraude wordt gepleegd. Vaak leveren de inzichten uit de datawetenschap goede voorspellingen op over mogelijke fraudegevallen. In de praktijk zijn er toch problemen, bijvoorbeeld met de steekproef en de data op basis waarvan voorspellingen worden gedaan. Dat probleem is sterker aanwezig naarmate de gebruikte modellen complexer zijn. In deze bijdrage wordt op deze problemen ingegaan en wordt betoogd dat menselijk handelen van groot belang is wanneer gebruik wordt gemaakt van voorspellende modellen.
Humor, Vernacularization, and Intermedial Laughter in Maoist Pingtan
Authors: Guo Li 
Published: May 2020

This chapter explores how humor in Maoist pingtan tales facilitated the vernacularization of the tastes and practices of popular culture, and revamped pingtan into an ideal media of socialist new comedy. Adaptations of pingtan tales through film, folk performances, and radio broadcasted songs facilitated the vernacularization of new notions of the self and the nation, generated intermedial laughter in various taste cultures and media territories, and instigated the individuals’ negotiation and interaction with multivalent socio-political ideals. Guo argues that vernacularization of Maoist pingtan, which allowed multiple styles to bleed into this classic storytelling art, was a process of necessity and accommodation in Maoist China when revolutionary linguistic codes are transposed into traditional arts, as well as the shifting relationships between individuals and the nation-state community.
Bioinformatics analyses of retinoblastoma reveal the retinoblastoma progression subtypes
Authors: Cao Manjing , Wang Sha , Zou Jing , Wang Wanpeng 
Published: May 2020

Introduction
Retinoblastoma (RB) is one common pediatric malignant tumor with dismal outcomes. Heterogeneity of RB and subtypes of RB were identified but the association between the subtypes of RB and RB progression have not been fully investigated.

Methods
Four public datasets were downloaded from Gene expression omnibus and normalization was performed to remove batch effect. Two public datasets were explored to obtain the RB progression gene signatures by differentially expression analysis while another two datasets were iterated for RB subtypes identification using consensus clustering. After the RB progressive subtype gene signatures were identified, we tested the diagnostic capacity of these gene signatures by receiver operation curve.

Results
Three hundreds and forty six genes that were enriched in cell cycle were identified as the progression signature in RB from two independent datasets. Four subtypes of RB were stratified by consensus clustering. A total of 21 genes from RB progression signature were differentially expressed between RB subtypes. One subtype with low expression cell division genes have less progression of all four subtypes. A panel of five RB subtype genes (CLUL1, CNGB1, ROM1, LRRC39 and RDH12) predict progression of RB.

Conclusion
Retinoblastoma is a highly heterogeneous tumor and the level of cell cycle related gene expression is associated with RB progression. A subpopulation of RB with high expression of visual perception has less progressive features. LRRC39 is potentially the RB progression subtype biomarker.
SMRT sequencing of the full-length transcriptome of the Rhynchophorus ferrugineus (Coleoptera: Curculionidae)
Authors: Yang Hongjun , Xu Danping , Zhuo Zhihang , Hu Jiameng , Lu Baoqian 
Published: May 2020

Background
Red palm weevil Rhynchophorus ferrugineus (Coleoptera: Curculionidae) is one of the most destructive insects for palm trees in the world. However, its genome resources are still in the blank stage, which limits the study of molecular and growth development analysis.

Methods
In this study, we used PacBio Iso-Seq and Illumina RNA-seq to first generate transcriptome from three developmental stages of R. ferrugineus (pupa, 7th larva, female and male) to increase our understanding of the life cycle and molecular characteristics of R. ferrugineus.

Results
A total of 63,801 nonredundant full-length transcripts were generated with an average length of 2,964 bp from three developmental stages, including the 7th instar larva, pupa, female adult and male adult. These transcripts showed a high annotation rate in seven public databases, with 54,999 (86.20%) successfully annotated. Meanwhile, 2,184 alternative splicing (AS) events, 2,084 transcription factors (TFs), 66,230 simple sequence repeats (SSR) and 9,618 Long noncoding RNAs (lncRNAs) were identified. In summary, our results provide a new source of full-length transcriptional data and information for the further study of gene expression and genetics in R. ferrugineus.
Transcriptome profiling of avian pathogenicEscherichia coliand the mouse microvascular endothelial cell line bEnd.3 during interaction
Authors: Wang Peili , Meng Xia , Li Jianji , Chen Yanfei , Zhang Dong , Zhong Haoran , Xia Pengpeng , Cui Luying , Zhu Guoqiang , Wang Heng 
Published: May 2020

BackgroundAvian pathogenicEscherichia coli(APEC), an important extraintestinal pathogenicE. coli, causes colibacillosis, an acute and mostly systemic disease involving multiple organ lesions such as meningitis. Meningitis-causing APEC can invade the host central nervous system by crossing the blood–brain barrier (BBB), which is a critical step in the development of meningitis. However, the bacteria-host interaction mechanism in this process remains unclear.MethodsIn this study, we examinedE. coliand bEnd.3 cells transcriptomes during infection and mock infection to investigate the global transcriptional changes in both organisms using RNA sequencing approach.ResultsWhen APEC infected the bEnd.3 cells, several significant changes in the expression of genes related to cell junctional complexes, extracellular matrix degradation, actin cytoskeleton rearrangement, immune activation and the inflammatory response in bEnd.3 cells were observed as compared to the mock infection group. Thus, the immune activation of bEnd.3 cells indicated that APEC infection activated host defenses. Furthermore, APEC may exploit cell junction degradation to invade the BBB. In addition, amino acid metabolism and energy metabolism related genes were downregulated and the protein export pathway related genes were upregulated in APEC cultured with bEnd.3 cells, compared to that in control. Thus, APEC may encounter starvation and express virulence factors during incubation with bEnd.3 cells.ConclusionThis study provides a comprehensive overview of transcriptomic changes that occur during APEC infection of bEnd.3 cells, and offers insights into the bacterial invasion strategies and the subsequent host defense mechanism.
Development and validation of a prognostic nomogram to predict overall survival and cancer-specific survival for patients with anaplastic thyroid carcinoma
Authors: Gui Weiwei , Zhu Weifen , Lu Weina , Shang Chengxin , Zheng Fenping , Lin Xihua , Li Hong 
Published: May 2020

BackgroundAnaplastic thyroid carcinoma (ATC) is a rare malignant tumor with a poor prognosis. However, there is no useful clinical prognostic predictive tool for ATC so far. Our study identified risk factors for survival of ATC and created a reliable nomogram to predict overall survival (OS) and cancer-specific survival (CSS) of patients with ATC.MethodsA total of 1,404 cases of ATC diagnosed between 1983 and 2013 were extracted from on the Surveillance, Epidemiology and End Results database based on our inclusion criteria. OS and CSS were compared among patients between each variable by Kaplan–Meier methods. The Cox proportional hazards model was used to evaluate multiple prognostic factors and obtain independent predictors. All independent risk factors were included to build nomograms, whose accuracy and practicability were tested by concordance index (C-index), calibration curves, ROC curves, DCA, net reclassification improvement (NRI) and integrated discrimination improvement (IDI).ResultsHistoric stage, tumor size, surgery and radiotherapy were independent risk factors associated with ATC according to multivariate Cox regression analysis of OS. However, gender was also an important prognostic predictor in CSS besides the factors mentioned above. These characteristics were included in the nomograms predicting OS and CSS of patients with ATC. The nomograms predicting OS and CSS performed well with a C-index of 0.765 and 0.773. ROC curves, DCA, NRI and IDI suggested that the nomogram was superior to TNM staging and age.ConclusionThe proposed nomogram is a reliable tool based on the prediction of OS and CSS for patients with ATC. Such a predictive tool can help to predict the survival of the patients.
Extensive protein expression changes induced by pamidronate in RAW 264.7 cells as determined by IP-HPLC
Authors: Lee Sang Shin , Kim Soung Min , Kim Yeon Sook , Lee Suk Keun 
Published: May 2020

Background
Bisphosphonate therapy has become a popular treatment for osteoporosis, Paget’s disease, multiple myeloma, osteogenesis imperfecta, myocardial infarction, and cancer despite its serious side effects. Bisphosphonate-induced molecular signaling changes in cells are still not clearly elucidated.

Methods
As bisphosphonates are primarily engulfed by macrophages, we treated RAW 264.7 cells (a murine macrophage cell line) with pamidronate and investigated global protein expressional changes in cells by immunoprecipitation high performance liquid chromatography (IP-HPLC) using 218 antisera.

Results
Pamidronate upregulated proliferation-activating proteins associated with p53/Rb/E2F and Wnt/β-catenin pathways, but downregulated the downstream of RAS signaling, pAKT1/2/3, ERK-1, and p-ERK-1, and subsequently suppressed cMyc/MAX/MAD network. However, in situ proliferation index of pamidronate-treated RAW264.7 cells was slightly increased by 3.2% vs. non-treated controls. Pamidronate-treated cells showed increase in the expressions of histone- and DNA methylation-related proteins but decrease of protein translation-related proteins. NFkB signaling was also suppressed as indicated by the down-regulations of p38 and p-p38 and the up-regulation of mTOR, while the protein expressions related to cellular protection, HSP-70, NRF2, JNK-1, and LC3 were upregulated. Consequently, pamidronate downregulated the protein expressions related to immediate inflammation,cellular differentiation, survival, angiogenesis, and osteoclastogenesis, but upregulated PARP-1 and FAS-mediated apoptosis proteins. These observations suggest pamidronate affects global protein expressions in RAW 264.7 cells by stimulating cellular proliferation, protection, and apoptosis but suppressing immediate inflammation, differentiation, osteoclastogenesis, and angiogenesis. Accordingly, pamidronate appears to affect macrophages in several ways eliciting not only its therapeutic effects but also atypical epigenetic modification, protein translation, RAS and NFkB signalings. Therefore, our observations suggest pamidronate-induced protein expressions are dynamic, and the affected proteins should be monitored by IP-HPLC to achieve the therapeutic goals during treatment.
3GPP Enhancements for Television Services: LTE‐Based 5G Terrestrial Broadcast
Authors: Gomez‐Barquero David , Gimenez Jordi J. , Beutler Roland 
Published: May 2020

AbstractThe provision of terrestrial broadcast services using 3GPP technologies was enabled for the first time in LTE Advanced Pro Release (Rel‐) 14, in which the evolved Multimedia Broadcast Multicast Service (eMBMS) technology was enhanced to fulfill a wide set of requirements input by the broadcast industry. During the Enhancements for Television (EnTV) work item in Rel‐14, several modifications were realized affecting system architecture, core, and radio access, which include, an interface to grant control to broadcasters to establish audiovisual services, an application programming interface for developers to simplify access to eMBMS procedures, or a transparent delivery mode with native content formats, among others. At the radio layer, dedicated carriers with 100% broadcast content allocation, and also new OFDM numerologies to support larger intersite distances in Single Frequency Networks (SFNs), were enabled. The most significant change was the so‐called receive‐only mode which enables devices receiving broadcast content without the need for uplink capabilities, SIM cards, or network subscriptions, i.e. free‐to‐air reception.In Rel‐16, 3GPP carried out a study item to evaluate EnTV Rel‐14 against the terrestrial broadcast requirements for 5G defined in 3GPP TR 38.913. Two requirements were detected as not met: the ability to support SFNs with cell radii of up to 100 km and mobile reception with speeds up to 250 km h−1. A Rel‐16 work item standardized further improvements while taking into account practical considerations such as implementation complexity and performance. The improved system is known as LTE‐based 5G Terrestrial Broadcast, and also with the popular name 5G Broadcast.
Posttranslational modification of Elongation Factor P from Staphylococcus aureus
Authors: Golubev Alexander , Negroni Luc , Krasnovid Filipp , Validov Shamil , Yusupova Gulnara , Yusupov Marat , Usachev Konstantin 
Published: May 2020

Antibiotic‐resistant Staphylococcus aureus is becoming a major burden on health care systems in many countries, necessitating the identification of new targets for antibiotic development. Elongation Factor P (EF‐P) is a highly conserved elongation protein factor that plays an important role in protein synthesis and bacteria virulence. EF‐P undergoes unique posttranslational modifications in a stepwise manner to function correctly, but experimental information on EF‐P posttranslational modifications is currently lacking for S. aureus. Here, we expressed EF‐P in S. aureus to analyze its posttranslational modifications by mass spectrometry and report experimental proof of 5‐aminopentanol modification of S. aureus EF‐P.