bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical Significance of Expression Level of CX3CL1-CX3CR1 Axis in Bone Metastasis of Lung Cancer
Authors: Liu Yan , Ma Hongmei , Dong Tiejun , Yan Yan , Sun Lixin , Wang Wenjie 
Published: April 2020

Abstract
Background: To investigate the clinical significance of CX3 chemokine ligand 1(CX3CL1) and CX3CR1 in patients with bone metastasis from lung cancer. The expression levels of CX3CL1 and CX3CR1 mRNA and protein in primary lung cancer and lung cancer bone metastasis were detected by qRT-PCR and Western blot. Methods: 100 patients with lung cancer were divided into a boneless metastasis group (50 patients with bone metastasis) and a bone metastasis group (50 patients without distant metastasis). The bone transfer component was graded by Soloway classification (0 to III). The expression levels of serum CX3CL1-CX3CR1 axis was detected by enzyme-linked immunosorbent assay (ELISA). RT-qPCR and Western Blot were used to verify the transfection efficiency. The scratching assay was used to detect the migration of CX3CL1 to 95-D cells after down-regulating the expression of CX3CR1. Results: The expression levels of CX3CL1 and CX3CR1 mRNA and protein in the primary lung cancer and lung cancer bone metastasis were significantly higher than those in the adjacent tissues (P<0.0001). The levels of serum CX3CL1 and CX3CR1 in bone metastasis group were significantly higher than those in boneless metastasis group and healthy control group (P<0.05). In the bone metastasis group, the levels of serum CX3CL1 and CX3CR1 was significantly positively correlated with the degree of disease progression (P<0.01). Conclusions: The expression level of serum CX3CL1-CX3CR1 axis is expected to be an auxiliary reference index for monitoring bone metastasis of lung cancer.
Down-regulation of Fine Dusts-induced Airway Inflammation by the PDZ motif peptide of ZO-1
Authors: Kang Dong Hee , Lee Tae-Jin , Kim Ji Wook , Shin Yu Som , Kim Ju Deok , Lee Ho Chul , Ryu Siejeong , Choi Yung Hyun , Kim Cheol Hong , You EunAe , Rhee SangMyung , Song Kyoung Seob 
Published: April 2020

Abstract
BACKGROUND: Although fine airborne particulate matter (PM) has been known to play a role in many human diseases, there is no direct evidence that therapeutic drugs or proteins can diminish PM-induced diseases. Nevertheless, studies examining the negative control mechanisms of PM-induced diseases are critical to develop novel therapeutic medications. RESULTS: We found that CLB2.0, a surrogate for PM, induced production of multiple cytokines that alter airway inflammation. Interestingly, deletion of each PDZ domain in the ZO-1 protein dramatically decreased F-actin formation and increased the expression of genes for pro-inflammatory cytokines. We also found that the consensus PDZ peptide in ZO-1 downregulates the expression of pro-inflammatory cytokine genes and F-actin formation; in contrast, the GG24,25AA mutant PDZ peptide upregulates them. Moreover, CLB2.0-induced F-actin formation in 2D and 3D matrix cultures was significantly inhibited by PDZ peptide, but not by the mutant peptide. Induction of IL-8 secretion by CLB2.0 activates CXCR2 signaling, while increased RGS12 controlled by the PDZ peptide inhibits IL-8/CXCR2 signaling. The consensus PDZ peptide also inhibited CLB2.0-induced inflammatory cell infiltration, pro-inflammatory cytokine gene expression, and TEER in bronchoalveolar lavage (BAL) fluid and AM cells. CONCLUSIONS: Our data indicated that the PDZ domain in ZO-1 is critical for regulation of the CLB2.0-induced inflammatory microenvironment. Therefore, we suggest that the PDZ peptide may be a potential therapeutic candidate during PM-induced respiratory diseases.
Association between laboratory results on day 8 of onset and the clinical outcome of COVID-19 patients
Authors: Shi Cuilin , Shi Meilin , Shen Jie , Xu Junchi , Ye Zhijian , Wang Xiafang , Yan Derui , Zeng Lingwu , Shen Xiujuan , Tang Zaixiang , Wu Meiying 
Published: April 2020

Abstract
Background: Pneumonia (COVID-19) caused by the novel coronavirus (2019-nCoV) outbreak attracted global attention. However, early prognostic biomarkers on clinical outcomes about COVID-19 patients is not clear by now. Methods: We collected the clinical data of the 85 COVID-19 patients confirmed in the local hospital. The patients' clinical baseline data, serological data, outcome data were retrospectively analyzed. This study aimed to figure out monitoring values for early prediction of clinical outcome. Results: Dynamic monitoring of laboratory examination indicators during hospitalization showed that the second week is a critical time window of disease progression. Beginning on the 8th day, severely reduced lymphocytes accompanied by a severe increase in inflammation indicators and abnormally high fibrinogen occurred in serious patients. Plasma fibrinogen was increased (5.23 vs 3.87 g/L, P < 0.001), and lymphocyte count (0.71 vs 1.42 109/L, P < 0.0001) was significantly reduced. On the 8th day of disease course, when the lymphocyte count was lower than 1.23×109/L, the time of negative conversion of viral nucleic acid was prolonged (P = 0.051), and imaging showed that the chest CT absorption time was prolonged (P < 0.01). When fibrinogen was greater than 4.24, the time to viral nucleic acid negative conversion was prolonged (P < 0.01), and imaging showed that chest CT absorption time was prolonged (P < 0.001).Conclusion: the Lymphocyte count might be related to clinical outcome events. It could act as laboratory indicators of disease severity.
The Co-Culture of ASCs and EPCs Promotes Vascularized Bone Regeneration in Critical-Sized Bone Defects of Cranial Bone in Rats
Authors: he yuanjia , Lin Shuang , Ao Qiang , He Xiaoning 
Published: April 2020

Abstract
Background: The repair of critical-sized bone defect represents a challenging problem in bone tissue engineering. To address the most important problem in bone defect repair, namely insufficient blood supply, this study aimed to find a method that can promote the formation of vascularized bone tissue.Method The phenotypes of ASCs and EPCs were identified respectively, and ASCs/EPCs were co-cultured in vitro to detect the expression of osteogenic and angiogenic genes. Furthermore, the co-culture system combined with scaffold material was used to repair the critical-sized bone defects of the cranial bone in rats.Results The co-culture of ASCs/EPCs could increase osteogenesis and angiogenesis-related gene expression in vitro. The results of in vivo animal experiments demonstrated that the ASCs/EPCs group could promote bone regeneration and vascularization in the meantime and, then significantly accelerate the repair of critical-sized bone defects.Conclusion It is feasible to replace traditional single seed cells with ASCs/EPCs co-culture system for vascularized bone regeneration. This system could ultimately enable clinicians to better repair the defect of craniofacial bone and avoid donor site morbidity.
Reduced KISS1 expression acts as a predictor for aggressive features of cervical carcinoma and promotes migration and invasion in cervical carcinoma cells
Authors: Wang Zhuo , Wang Min , Yu Hao , Wang Hongyu , Wang Can , Ou Jian , Meng Fanxu , Zhang Yang 
Published: April 2020

Abstract
Background

Cervical carcinoma causes high morbidity and mortality in patients largely due to its invasion and metastasis. Kisspeptin (KISS1) had been found as metastasis suppressor in many malignancies. But the clinical significance and biological functions of KISS1 in cervical carcinoma was not elaborated.
Methods

The expression levels of KISS1 were detected by quantitative real time polymerase chain reaction (qRT-PCR), Methylation specific PCR (MSP) analysis and western-blotting in cervical carcinoma tissues and cells. A eukaryotic expression plasmid was transfected into cervical epithelial cells in order to initiate KISS1 overexpression, and the endogenous was silenced in cervical epithelial cells using short hairpin RNA (shRNA) sequences. Transfection efficiency was validated by western blotting assays. To explore the effects of KISS1 on the metastatic phenotype of cervical carcinoma, the cell proliferation was examined by CCK8 assay, and the cell migration and invasion were detected by cell scratch and transwell invasion assays, respectively.
Results

We investigated the relevance between KISS1 expression with its methylation and clinicopathological features of cervical carcinoma. Low expression of KISS1 predicts a poor prognosis and was associated with lymph node metastasis and TNM stage. And methylation of KISS1 promoter, which was linked with KISS1 inactivation, was also found in most clinical samples of aggressive cervical carcinoma. In vitro cervical carcinoma C33A cells, KISS1 overexpression significantly inhibited cell proliferation, migration and invasion in cells. While KISS1 knockdown promoted proliferation, migration and invasion in cervical epithelial cell line CRL-2614.
Conclusion

In summary, low-expression of KISS1 play a suppressive role for proliferation, migration and invasion in cervical carcinoma cells. Its expression and methylation are associated with clinical progression of cervical carcinoma. Hence, KISS1 could be a potential diagnostic and prognostic marker as well as a new target for the treatment of cervical carcinoma.
In vitro elution and E. coli and T. pyogenes killing profile of penicillin, ampicillin, tetracycline, tulathromycin, and florfenicol from Plaster of Paris beads
Authors: Merkatoris Paul , Schleining Jennifer , Krull Adam , Borts David , Fajt Virginia 
Published: April 2020

Abstract
Background: The objectives of this study were to create a protocol for making antibiotic impregnated Plaster of Paris (AI-PoP) beads using penicillin, ampicillin, tetracycline, tulathromycin, and florfenicol, to determine the in vitro elution rates of those antibiotics in the beads, and to quantify the in vitro inhibition of E. coli and T. pyogenes by the bead eluent in a proof-of-concept study using flow cytometry. The AI-PoP beads were made using Plaster of Paris powder, antibiotic, and tap water, cured for 24 hours, sterilized by ethylene oxide, and stored up to 5 months before testing. For each antibiotic, twenty beads were combined with bovine serum in sterile tubes and incubated at 37° C on a rocker. Serum was replaced at intervals over the 14 day study period, and antibiotic concentrations were determined by high pressure liquid chromatography with mass spectrometry. Separately, the growth of E. coli and T. pyogenes in eluent from 10 beads for each antibiotic was quantified by flow cytometry. Results: Antibiotic was detected in AI-PoP bead eluent for 14 days for all but the ampicillin beads, for which antibiotic was detected for 8 days. The concentration of antibiotic in eluent was greater than five times the minimum inhibitory concentration (MIC) of tested bacteria for the entire study period for penicillin, tetracycline, and florfenicol. The concentration of ampicillin remained greater than five times the MIC of E. coli for 4 days and T. pyogenes for 5 days. The concentration of tulathromycin remained greater than five times the MIC of E. coli for 5 days and T. pyogenes for the entire study period. The CFU/ml of live E. coli and T. pyogenes was reduced over a 72-hour period by 1-3 log 10 CFU, with the exception of tetracycline, which reduced CFU/ml of T. pyogenes by less than log 10 CFU. Conclusions: AI-PoP beads containing penicillin, tetracycline, tulathromycin and florfenicol elute antibiotic well above the MIC of tested bacteria for the two week study period and provide adequate local bacterial growth inhibition to reduce bacterial growth in vitro . These antibiotics show promise for delivery in joint and wound infections.
Identification of a four long non-coding RNA (lncRNA) Signature for Predicting Prognosis of Patients with Non-Small Cell Lung Cancer: a Multicenter Study in China
Authors: Wang Rui-Qi , Long Xiao-Ran , Ge Chun-Lei , Zhang Mei-Yin , Huang Long , Zhou Ning-Ning , Hu Yi , Li Rui-Lei , Li Zhen , Chen Dong-Ni , Zhang Lan-Jun , Wen Zhe-Sheng , Mai Shi-Juan , WANG HUI-YUN 
Published: April 2020

Abstract
Background:This study aims to identify a long non-coding RNA (lncRNA) signature for predicting survival in non-small-cell lung carcinoma (NSCLC) patients and providing additional prognostic information to the tumor node metastasis (TNM) staging system. Methods: NSCLC cases from a hospital were divided into a discovery cohort (n=194) and validation cohort (n=172) and analyzed using a custom lncRNA microarray. Another 73 cases obtained from another hospital were assayed using quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). The differentially expressed lncRNAs were detected by significance analysis of microarrays (SAM) program and used for identifying those associated with survival in the discovery cohort, which were then employed to construct a prognostic lncRNA signature using a risk-score method. The signature was then confirmed in the validation and independent cohort as well. Results: The discovery cohort was found to comprise of 305 lncRNAs, which showed differential expression between the NSCLC and the corresponding normal lung tissues, a 4-lncRNA signature was identified that was found to significantly correlate with the survival of the NSCLC patients. This signature was further validated in the validation and independent cohort. Moreover, multivariate Cox analysis demonstrates that the 4-lncRNA signature is independent of the TNM staging system.as a risk-score model. The receiver operating characteristic (ROC) curve indicates that the prognostic value of the combined model is significantly higher than that of TNM staging alone in all the cohorts. Conclusions:This study identified a 4-lncRNA signature, which is a powerful prognostic biomarker which related to patient survival in addition to the traditional TNM staging system.
The diagnostic power of CD117, CD13, CD56, CD64, and MPO in rapid screening acute promyelocytic leukemia
Authors: Tran Vinh Thanh , Phan Thang Thanh , Mac Hong-Phuoc , Tran Tung Thanh , Ho Toan Trong , Pho Suong Phuoc , Nguyen Van-Anh Ngoc , Vo Truc-My , Nguyen Hue Thi , Le Thao Thi , Vo Tin Huu , Nguyen Son Truong 
Published: April 2020

Abstract
Objective
The analogous immunophenotype between HLA-DR-negative acute myeloid leukemia (AML) and acute promyelocytic leukemia (APL) causes APL rapid screening to become difficult. This study aimed to identify the associated antigens for APL and the best model in clinical uses.
Results
A total of 36 APL (PML-RARA+) and 29 HLA-DR-negative non-APL patients were selected for this study. When a cut-off point of 20% events was applied to define positive or negative status, APL and non-APL patients share a similar immunophenotype of CD117, CD34, CD11b, CD13, CD33, and MPO (P > 0.05). However, expression intensity of CD117 (P = 0.002), CD13 (P < 0.001), CD35 (P < 0.001), CD64 (P < 0.001), and MPO (P < 0.001) in APL are significantly higher while CD56 (P = 0.049) is lower than in non-APL subjects. The Bayesian Model Averaging (BMA) analysis identified CD117 (≥ 49% events), CD13 (≥ 88% events), CD56 (≤ 25% events), CD64 (≥ 42% events), and MPO (≥ 97% events) antigens as an optimal model for APL diagnosis. A combination of these factors resulted in an area under curve (AUC) value of 0.98 together with 91.7% sensitivity and 93.1% specificity, which is higher than individual values (AUC were 0.76, 0.84, 0.65, 0.82, and 0.85, respectively) (P = 0.001).
Pediatric neurodevelopment by prenatal Zika virus exposure: A cross-sectional study of the Microcephaly Epidemic Research Group Cohort
Authors: Silva paula sobral , Eickmann Sophie Helena , Ximenes Ricardo Arraes de Alencar , Montarroyos Ulisses Ramos , Lima Marília de Carvalho , Martelli Celina M Turchi , de Araújo Thalia Velho Barreto , Brickley Elizabeth , Rodrigues Laura Cunha , Gonçalves Fabiana Cristina Lima Pastich , Carvalho Maria Durce Costa Gomes , de Souza Wayner Vieira , Miranda-Filho Democrito de Barros 
Published: April 2020

Abstract
Background
The implications for pediatric neurodevelopment and behavior remain sparsely studied in children exposed to congenital infection by Zika Virus (ZIKV). The aim of this study is to compare patterns of neurodevelopment and behavior of children with different levels of Zika-related microcephaly or prenatal exposure without microcephaly.
Methods
A Cross-sectional study nested in a cohort, including 274 children aged 10–45 months. Participantes were evaluated between Feb/2017 and Aug/2019 in two tertiary care hospitals in Recife/Pernambuco/Brazil. Primary exposure was having ZIKV-related microcephaly or prenatal ZIKV-exposure, confirmed by maternal RT-PCR testing. We included children born during the peak of microcephaly epidemic and post epidemic period, divided in four groups according to clinical and laboratory criteria. Groups 1 and 2 had microcephaly, severe and moderate, respectively. Group 3 was composed of children with laboratory evidence of prenatal ZIKV-exposure and no microcephaly, and Group 4 was a neurotypical control group. We used the Survey of Wellbeing of Young Children (SWYC), a screening instrument validated for Brazil, and an SWYC Adapted Form to compare severe cases.
Results
Almost all children with severe microcephaly presented neurological abnormalities (98.2%) and risk of development delay (99.1%). The frequency of risk of development delay for the group with moderate microcephaly was 65%. For children without microcephaly, the risk varied from 13.8–21.7%. There was no difference in behavior aspects among groups. According to the Adapted Form, the majority of the children with severe microcephaly scored much lower than expected, and presented similar performance whether evaluated under or over 24 months of age.
Conclusions
We found a gradient of risk of development delay among groups, being the group with severe microcephaly at the highest risk and the groups of normocephalic exposed children and controls the lowest ones. The Adapted Form was useful to estimate children deficit profile and showed no significant improvement in ability regardless age of assessment. We suggest that ZIKV-exposed children should undergo first-line screening for neurodevelopment and behavior using the SWYC instrument. Early assessment and follow-up will enable at-risk children to be referred to a more comprehensive developmental evaluation and to multidisciplinary care management.
Comfort evaluation of aircraft cabin system employing a hybrid model
Authors: LIU Jing , YU Suihuai , CHU Jianjie 
Published: April 2020

Abstract
Comfort is becoming one of the most important principles in the process of design and evaluation of civil aircraft cabin. However, the comprehensive quantitative evaluation of comfort in an aircraft cabin is a complicated issue, because of the subjectivity of comfort perception and a large amount of factors involved in the whole complex cabin system. A hybrid model combined with Decision Making Trial and Evaluation Laboratory (DEMATEL) method and fuzzy comprehensive evaluation is proposed, which considers both the interrelation between the criteria and the fuzziness of subjective comfort perception concurrently. The result of empirical study from questionnaire survey in flight was consistent with that of the hybrid model. The proposed model is effective. It could provide a more reasonable priority to improve comfort in the aircraft cabin. According to the measured results of cabin environment, cabin facilities and layout, seat and service, the specific differences between the criteria can be displayed clearly, which is helpful to improve the cabin comfort level.
Anopheles arabiensis and Anopheles funestus s.s. biting pattern in Dielmo, an area of low level exposure to malaria vectors.
Authors: Doucoure Souleymane , Thiaw Omar , Wotodjo Amélé N. , Bouganali Charles , Diagne Nafissatou , Parola Philippe , Sokhna Cheikh 
Published: April 2020

Abstract
Background: In Dielmo, Senegal, the widespread use of long lasting insecticidal nets has decreased both the incidence of malaria and the density of the anopheles population. However, persistent low-level malaria transmission may hamper efforts to eliminate the disease. Therefore, continuous monitoring of the vector population is needed in order to improve knowledge of anopheles biting behaviour and to readjust control interventions.Methods: In 2015, anopheles were collected every month for a whole year and each specimen was identified using morphological and molecular techniques. The biting pattern of each species was analysed according to night (7pm–7am) and day (7am–11am) periods, the place of biting and the season. The ELISA CSP technique was used to evaluate the entomological inoculation rate (EIR).Results: An. arabiensis and An. funestus s.s. were found to be the main vectors biting human in Dielmo. Overall, the biting rate was low, at 3.84bites per night (bpn) and 1.27 bites per day (bpd), respectively. The EIR was 2.51 and 5.03 infected bites per year during the night and day, respectively. During the night, the An. arabiensis and An. funestus biting rate was 1.81 bpn and 1.71 bpn, respectively (p=0.80). During daylight hours, their aggressiveness decreased to 0.51 bpd and 0.73 bpd for An. arabiensis and An. funestus, respectively (p=0.30). During the night, depending on the seasons, only An. funestus displayed a significant outdoor biting behaviour both in the rainy and dry seasons, while during the day, no specific trend of indoor or outdoor biting was observed, regardless of the season for both vectors.Conclusion: This study highlighted low level anopheles nocturnal and diurnal biting and the associated risk of malaria transmission. It showed also the influence of the season on the indoor and outdoor biting pattern, indicating that the human population could be exposed all year round to a low level of anopheles bites. Control programs should increase awareness of the use of bed-nets throughout the year, particularly shortly after dawn when people are still indoors.
Prostate cancer detection improved by optimization of biopsy strategy: Bayesian framework of Network Meta -Analysis and HSROC model for diagnostic accuracy
Authors: Rahman Ilham Akbar , Fauzan Ilham , Syahrir Syakri , Nusaly Harry , Kasim Firdaus 
Published: April 2020

Abstract
Background: The increasing problem in prostate cancer is associated with over-diagnosis and over-treatment. The transrectal ultrasonography (TRUS)-guided biopsy as a current gold standard investigation poses low positive detection rate causing unnecessary biopsies. The choice of optimal biopsy strategy needs to be completely defined. Therefore, in the absence of direct statistical comparison and limited evidence, we undertook the advantage of Bayesian network meta-analysis and Bayesian prediction in the HSROC model to present a method for optimizing biopsy strategy in prostate cancer. Materials and Methods: Relevant 24 studies were comprehensively retrieved through online databases of EMBASE, MEDLINE, and CENTRAL up to February 2020. Markov chain Monte Carlo simulation and SUCRA were used to calculate the rank probability using odds ratio (OR) with 95% CI. HSROC model was used to formulate the predicted true sensitivity and specificity of each biopsy strategy. Data analysis was performed using STATA, R, and WinBUGS statistics. Results: Six different PCa biopsy strategies based on four outcomes were ultimately analyzed in this study with a total of 6768 patients. The rank probability showed that the overall PCa detection rate was higher in FUS+TRUS-GB, MRI-GB, and FUS-GB. In terms of clinically significant PCa detection, FUS+TRUS-GB, and FUS-GB had a relatively higher clinically significant detection rate. Moreover, MRI-GB, FUS-GB, and FUS+TRUS-GB were less associated with insignificant PCa detection rate. MRI-GB and FUS-GB had the highest predicted of true sensitivity and specificity. Conclusion: Taken together, in comprehensive consideration of four endpoints, our analysis highlights the important finding that FUS+TRUS-GB, FUS-GB, and MRI-GB showed their superiority compared with other strategies. TRUS-GB was more associated with over-diagnosis of insignificant PCa, lower sensitivity, and specificity.