bluuebunny/crossref_metadata_2025_split huggingface dataset:
Moderate alcohol intake promotes pancreatic ductal adenocarcinoma development in mice expressing oncogenic Kras
Authors: Asahina Kinji , Balog Steven , Hwang Edward , Moon Eugene , Wan Emily , Skrypek Kaitlin , Chen Yibu , Fernandez Jay , Romo Janet , Yang Qihong , Lai Keane , French Samuel W. , Tsukamoto Hidekazu 
Published: November 2019

Kras mutations are associated with pancreatic ductal adenocarcinoma (PDAC). Although tobacco smoking, pancreatitis, and obesity are known environmental risk factors for PDAC, the contribution of moderate alcohol intake to PDAC remains elusive. In the present study, we tested whether a combination of risk factors or moderate alcohol intake induces PDAC development in mice. Control Pdx1Cre and Pdx1Cre;LSL- KrasG12D mutant mice were fed a Western alcohol diet containing high levels of cholesterol and saturated fat, 3.5% alcohol, and lipopolysaccharide for 5 mo. In addition, mice were treated with cerulein, for induction of pancreatitis, and nicotine every month. Treatment with all of these risk factors promoted development of advanced pancreatic neoplasia and PDAC in the Pdx1Cre;LSL- KrasG12D mice but not in the control Pdx1Cre mice. Moderate alcohol intake or Western diet feeding also significantly promoted advanced neoplasia and PDAC development in Pdx1Cre;LSL- KrasG12D mice compared with mice fed a regular chow. Alcohol, but not Western diet, increased tumor development in the liver in the Pdx1Cre;LSL- KrasG12D mice, but its origin remained elusive due to leakiness of Pdx1Cre in hepatocytes. RNA-seq analysis revealed that alcohol feeding increases expression of markers for tumors ( Epcam, Krt19, Prom1, Wt1, and Wwtr1), stroma ( Dcn, Fn1, and Tnc), and cytokines ( Tgfb1 and Tnf) and decreases expression of Fgf21 and Il6 in the pancreatic tumor tissues. Immunostaining showed heterogeneous expression of nephronectin, S100 calcium-binding protein A6, and vascular cell adhesion molecule 1 in pancreatic tumors surrounded by podoplanin-positive stromal cells. Our data indicate that moderate alcohol drinking is a risk factor for development of PDAC. NEW & NOTEWORTHY Heavy alcohol intake has been suspected to be a risk factor of pancreatic ductal adenocarcinoma (PDAC) in humans. However, the contribution of moderate alcohol intake to PDAC development remains elusive. In the present study, we experimentally show that moderate alcohol feeding significantly induces advanced stages of pancreatic intraepithelial neoplasia development and invasive PDAC in Pdx1Cre;LSL- KrasG12D mutant mice. Our data indicate that moderate alcohol drinking is a risk factor for PDAC.
Catheter Ablation Versus Best Medical Therapy in Patients With Persistent Atrial Fibrillation and Congestive Heart Failure
Authors: Kuck Karl-Heinz , Merkely Béla , Zahn Ralf , Arentz Thomas , Seidl Karlheinz , Schlüter Michael , Tilz Roland Richard , Piorkowski Christopher , Gellér László , Kleemann Thomas , Hindricks Gerhard 
Published: November 2019

Background:
Optimal treatment of patients with persistent atrial fibrillation (AF) and heart failure (HF) with reduced left ventricular ejection fraction (LVEF) and an indication for internal defibrillator therapy is controversial.

Methods:
Patients with persistent/longstanding persistent AF and LVEF ≤35% were randomly allocated to catheter ablation of AF or best medical therapy (BMT). The primary study end point was the absolute increase in LVEF from baseline at 1 year. Secondary end points included 6-minute walk test, quality-of-life, and NT-proBNP (N-terminal pro-brain natriuretic peptide). Pulmonary vein isolation was the primary ablation approach; BMT comprised rate or rhythm control. All patients were discharged after index hospitalization with a cardioverter-defibrillator or cardiac resynchronization therapy defibrillator implanted. The study was terminated early for futility.

Results:

Of 140 patients (65±8 years, 126 [90%] men) available for the end point analysis, 68 and 72 patients were assigned to ablation and BMT, respectively. At 1 year, LVEF had increased in ablation patients by 8.8% (95% CI, 5.8%–11.9%) and in BMT patients by 7.3% (4.3%–10.3%;
P
=0.36). Sinus rhythm was recorded on 12-lead electrocardiograms at 1 year in 61/83 ablation patients (73.5%) and 42/84 BMT patients (50%). Device-recorded AF burden at 1 year was 0% or maximally 5% of the time in 28/39 ablation patients (72%) and 16/36 BMT patients (44%). There was no difference in secondary end point outcome between ablation patients and BMT patients.

Conclusions:
The AMICA trial (Atrial Fibrillation Management in Congestive Heart Failure With Ablation) did not reveal any benefit of catheter ablation in patients with AF and advanced HF. This was mainly because of the fact that at 1 year, LVEF increased in ablation patients to a similar extent as in BMT patients. The effect of catheter ablation of AF in patients with HF may be affected by the extent of HF at baseline, with a rather limited ablation benefit in patients with seriously advanced HF.

Clinical Trial Registration:

URL:
https://www.clinicaltrials.gov
. Unique identifier: NCT00652522.
Do CMIP5 Models Show El Niño Diversity?
Authors: Feng Jie , Lian Tao , Ying Jun , Li Junde , Li Gen 
Published: November 2019

AbstractWhether the state-of-the-art CMIP5 models have different El Niño types and how the degree of modeled El Niño diversity would be impacted by the future global warming are still heavily debated. In this study, cluster analysis is used to investigate El Niño diversity in 30 CMIP5 models. As the method does not rely on any prior knowledge of the patterns of El Niño seen in observations, it provides a practical way to identify the degree of El Niño diversity in models. Under the historical scenario, most models show a poor degree of El Niño diversity in their own model world, primarily due to the lopsided numbers of events belonging to the two modeled El Niño types and the weak compactness of events in each cluster. Four models are found showing significant El Niño diversity, yet none of them captures the longitudinal distributions of the warming centers of the two El Niño types seen in the observations. Heat budget analysis of the sea surface temperature (SST) anomaly suggests that the degree of modeled El Niño diversity is highly related to the climatological zonal SST gradient over the western-central equatorial Pacific in models. As the gradient is weakened in most models under the future high-emission scenario, the degree of modeled El Niño diversity is further reduced in the future. The results indicate that a better simulation of the SST gradient over the western-central equatorial Pacific might allow a more reliable simulation/projection of El Niño diversity in most CMIP5 models.
Spatiotemporal Variability of Tropical Cyclone Precipitation Using a High-Resolution, Gridded (0.25° × 0.25°) Dataset for the Eastern United States, 1948–2015
Authors: Bregy Joshua C. , Maxwell Justin T. , Robeson Scott M. , Ortegren Jason T. , Soulé Peter T. , Knapp Paul A. 
Published: November 2019

AbstractTropical cyclones (TCs) are an important source of precipitation for much of the eastern United States. However, our understanding of the spatiotemporal variability of tropical cyclone precipitation (TCP) and the connections to large-scale atmospheric circulation is limited by irregularly distributed rain gauges and short records of satellite measurements. To address this, we developed a new gridded (0.25° × 0.25°) publicly available dataset of TCP (1948–2015; Tropical Cyclone Precipitation Dataset, or TCPDat) using TC tracks to identify TCP within an existing gridded precipitation dataset. TCPDat was used to characterize total June–November TCP and percentage contribution to total June–November precipitation. TCP totals and contributions had maxima on the Louisiana, North Carolina, and Texas coasts, substantially decreasing farther inland at rates of approximately 6.2–6.7 mm km−1. Few statistically significant trends were discovered in either TCP totals or percentage contribution. TCP is positively related to an index of the position and strength of the western flank of the North Atlantic subtropical high (NASH), with the strongest correlations concentrated in the southeastern United States. Weaker inverse correlations between TCP and El Niño–Southern Oscillation are seen throughout the study site. Ultimately, spatial variations of TCP are more closely linked to variations in the NASH flank position or strength than to the ENSO index. The TCP dataset developed in this study is an important step in understanding hurricane–climate interactions and the impacts of TCs on communities, water resources, and ecosystems in the eastern United States.
Rapid Arctic Sea Ice Loss on the Synoptic Time Scale and Related Atmospheric Circulation Anomalies
Authors: Wang Zhuo , Walsh John , Szymborski Sarah , Peng Melinda 
Published: November 2019

AbstractLarge sea ice loss on the synoptic time scale is examined in various subregions in the Arctic as well as at the pan-Arctic scale. It is found that the frequency of large daily sea ice loss (LDSIL) days is significantly correlated with the September sea ice extent over the Beaufort–Chukchi–Siberian Seas, the Laptev–Kara Seas, the central Arctic, and the all-Arctic regions, indicating a link between the synoptic sea ice variability and the interannual variability of the annual minimum sea ice extent. A composite analysis reveals dipoles of anomalous cyclones and anticyclones associated with LDSIL days. Different from the well-known Arctic dipole pattern, the east–west dipoles are found over the corresponding regions of LDSIL in the Arctic marginal seas and are associated with the increasing occurrence of Rossby wave breaking and atmospheric rivers. The anticyclones of the dipoles are persistent and quasi-stationary, reminiscent of blocking. The anomalous poleward flow between the cyclone and the anticyclone enhances the poleward transport of heat and water vapor in the lower troposphere. Although enhanced downward shortwave radiation, associated with reduced cloud fraction, is found in some regions, it is not collocated with the regions of LDSIL. In contrast, enhanced downward longwave radiation owing to increasing column water vapor shows good spatial correspondence with LDSIL, indicating the importance of atmospheric rivers in LDSIL events. Lead/lag composites with respect to the onset of LDSIL episodes reveal precursor wave trains spanning the midlatitudes. The wave trains have predominantly zonal energy propagation in the midlatitudes and do not show a clear link to tropical or subtropical forcing.
Hepatitis C–Related Mortality Among American Indian/Alaska Native Persons in the Northwestern United States, 2006-2012
Authors: Hatcher Sarah M. , Joshi Sujata , Robinson Byron F. , Weiser Thomas 
Published: November 2019

Objective: American Indian and Alaska Native (AI/AN) persons are commonly misclassified in epidemiologic and administrative data sets. The race-corrected hepatitis C virus (HCV)–related mortality rate among AI/AN persons in the Northwest United States (Idaho, Oregon, and Washington State) is unknown. We quantified the disparity in HCV-related mortality between AI/AN persons and non-Hispanic white (NHW) persons in the Northwest during 2006-2012 after correcting misclassified AI/AN race. Methods: After conducting probabilistic record linkage between death records and the Northwest Tribal Registry, we calculated HCV-related mortality rates for AI/AN and NHW persons by using National Center for Health Statistics bridged-race estimates standardized to the US 2000 standard population. Results: The 2006-2012 aggregate age-adjusted HCV-related mortality rate per 100 000 population in the Northwest was 19.6 (95% confidence interval [CI], 17.3-22.2) for AI/AN persons and 5.9 (95% CI, 5.7-6.1) for NHW persons (rate ratio [RR] = 3.3; 95% CI, 3.0-3.8). The disparity was larger among females (RR = 4.6; 95% CI, 3.8-5.5) than among males (RR = 2.9; 95% CI, 2.5-3.4). Conclusion: The disproportionate rate of HCV-related mortality among AI/AN persons in the Northwest highlights the need to expand HCV education, screening, and treatment among this population.
Engagement of the Secondary Ligamentous and Meniscal Restraints Relative to the Anterior Cruciate Ligament Predicts Anterior Knee Laxity
Authors: Kent Robert N. , Imhauser Carl W. , Thein Ran , Marom Niv , Wickiewicz Thomas L. , Nawabi Danyal H. , Pearle Andrew D. 
Published: November 2019

Background: Patients with high-grade preoperative side-to-side differences in anterior laxity as assessed via the Lachman test after unilateral anterior cruciate ligament (ACL) rupture are at heightened risk of early ACL graft failure. Biomechanical factors that predict preoperative side-to-side differences in anterior laxity are poorly understood. Purpose: To assess, in a cadaveric model, whether the increase in anterior laxity caused by sectioning the ACL (a surrogate for preoperative side-to-side differences in anterior laxity) during a simulated Lachman test is associated with two biomechanical factors: (1) the tibial translation at which the secondary anterior stabilizers, including the remaining ligaments and the menisci, begin to carry force, or engage, relative to that of the ACL or (2) the forces carried by the ACL and secondary stabilizers at the peak applied anterior load. Study Design: Controlled laboratory study. Methods: Seventeen fresh-frozen human cadaveric knees underwent Lachman tests simulated through a robotic manipulator with the ACL intact and sectioned. The net forces carried by the ACL and secondary soft tissue stabilizers (the medial meniscus and all remaining ligaments, measured as a whole) were characterized as a function of anterior tibial translation. The engagement points of the ACL (with the ACL intact) and each secondary stabilizer (with the ACL sectioned) were defined as the anterior translation at which they began to carry force, or engaged, during a simulated Lachman test. Then, the relative engagement point of each secondary stabilizer was defined as the difference between the engagement point of each secondary stabilizer and that of the ACL. Linear regressions were performed to test each association ( P < .05). Results: The increase in anterior laxity caused by ACL sectioning was associated with increased relative engagement points of both the secondary ligaments (β = 0.87; P < .001; R2 = 0.75) and the medial meniscus (β = 0.66; P < .001; R2 = 0.58). Smaller changes in anterior laxity were also associated with increased in situ medial meniscal force at the peak applied load when the ACL was intact (β = −0.06; P < .001; R2 = 0.53). Conclusion: The secondary ligaments and the medial meniscus require greater anterior tibial translation to engage (ie, begin to carry force) relative to the ACL in knees with greater changes in anterior laxity after ACL sectioning. Moreover, with the ACL intact, the medial meniscus carries more force in knees with smaller changes in anterior laxity after ACL sectioning. Clinical Relevance: Relative tissue engagement is a new biomechanical measure to characterize in situ function of the ligaments and menisci. This measure may aid in developing more personalized surgical approaches to reduce high rates of ACL graft revision in patients with high-grade laxity.
Immediate Release of Vitreomacular Traction After Pneumatic Vitreolysis Followed by the Drinking Bird Technique
Authors: Mehta Neesurg , Gupta Lalita , Jansen Michael , Rosen Richard , Lee Jessica 
Published: November 2019

Purpose: To report a case of release of vitreomacular traction (VMT) in a patient with a full thickness macular hole (FTMH) immediately following pneumatic vitreolysis (PV) combined with head bobbing movements. Methods: A 71-year-old female with VMT and an FTMH presented with blurred vision for 2 months to the level of 20/400. At her 1-month follow-up visit, PV was performed using C3F8 gas and she was instructed to perform the drinking bird technique for ten minutes. Results: Optical coherence tomography performed ten minutes after PV with head bobbing showed VMT release and a smaller FTMH. Visual acuity improved to 20/150 immediately afterwards and to 20/80 two months later. Conclusions: Using the drinking bird technique for a continuous period of time immediately following PV may encourage rapid VMT release. PV may be a feasible option for patients with VMT and FTMH who do not want surgery.
TS-GOEA: a web tool for tissue-specific gene set enrichment analysis based on gene ontology
Authors: Peng Jiajie , Lu Guilin , Xue Hansheng , Wang Tao , Shang Xuequn 
Published: November 2019

AbstractBackgroundThe Gene Ontology (GO) knowledgebase is the world’s largest source of information on the functions of genes. Since the beginning of GO project, various tools have been developed to perform GO enrichment analysis experiments. GO enrichment analysis has become a commonly used method of gene function analysis. Existing GO enrichment analysis tools do not consider tissue-specific information, although this information is very important to current research.ResultsIn this paper, we built an easy-to-use web tool calledTS−GOEAthat allows users to easily perform experiments based on tissue-specific GO enrichment analysis.TS−GOEAuses strict threshold statistical method for GO enrichment analysis, and provides statistical tests to improve the reliability of the analysis results. Meanwhile,TS−GOEAprovides tools to compare different experimental results, which is convenient for users to compare the experimental results. To evaluate its performance, we tested the genes associated with platelet disease withTS−GOEA.ConclusionsTS−GOEAis an effective GO analysis tool with unique features. The experimental results show that our method has better performance and provides a useful supplement for the existing GO enrichment analysis tools.TS−GOEAis available athttp://120.77.47.2:5678.
The practice of key essential nutrition action messages and associated factors among mothers of children from birth up to 2 years old in Wereilu Wereda, south Wollo zone, Amhara, Northeast Ethiopia: a community based cross-sectional study
Authors: Beletew Biruk , Gebremichael Bereket , Tesfaye Tewodros , Mengesha Ayelign , Wudu Mesfin 
Published: November 2019

Abstract
Background
The 1000 days, between a woman’s pregnancy and her child’s second birthday, offer a unique window of opportunity to build healthier and more prosperous societies. The right nutrition during this time can have a profound impact on a child’s ability to grow, learn, and rise out of poverty. An essential nutrition action is one of the most effective preventive actions for reducing nutrition-related morbidity and mortality among young children. Nonetheless, there is limited evidence regarding the practice of essential nutrition action and its associated factors.

Objective
The main aim of this study was to assess the practice of key essential nutrition action messages and associated factors among mothers of children from birth up to 2 years old in Wereilu Wereda, South Wollo Zone, Northeast Ethiopia.

Methods
A community-based cross-sectional study was conducted on 563 mothers of children from birth up to 2 years old in Wereilu Wereda from April 1–30, 2018. A multi-stage sampling technique was employed. A structured, adapted and pre-tested questionnaire was used to collect the data. The data was collected through the interviews. The data were entered in EPI-data Version 4.2 and exported to SPSS version 24.0 for analysis. Bivariate and multivariable logistic regression was carried out to asses’ associations between the outcome and independent variables. A p-value of < 0.05 was considered statistically significant in this study.

Results
The prevalence of good practice was 256 (46.5%). Educational status of the mother and the father, monthly income, parity, place of birth, postnatal care follow-up, level of knowledge and level of attitude were predictors of good practice.

Conclusion
The practices of key essential nutrition action messages in the study area were found to be low. So, it is better to improve access to information to the community on key essential nutrition action messages through trained health workers coupled with media.
Association between 90o push-up and cardiorespiratory fitness: cross-sectional evidence of push-up as a tractable tool for physical fitness surveillance in youth
Authors: Ajisafe Toyin 
Published: November 2019

Abstract
Background
Despite being associated with health outcomes like abdominal adiposity, depression, anxiety, and cardiovascular disease risk among youth, largely, clinicians still do not adopt physical fitness testing. A clarion call for increased surveillance was previously issued, in order to address the US population-level lack of knowledge regarding pervasive inactivity among children. Because schools often do not send home annual physical fitness testing results, many lay parents are unaware of their child’s physical fitness or the risk of associated adverse health outcomes. This study investigated associations between musculoskeletal fitness measures (including 90o push-up), cardiorespiratory fitness, and weight status.

Methods
Two hundred and ten students (9.7 ± 1.08 years, 138.6 ± 9.4 cm; 42.3 ± 14.4 kg) across third through fifth grades were tested for cardiorespiratory (i.e., Progressive Aerobic Cardiovascular Endurance Run (PACER)) and musculoskeletal (90o push-up, trunk lift, sit-and-reach and curl-up) fitness. The relationships between measures of musculoskeletal and cardiorespiratory fitness were modeled using a series of linear regression analyses. Models were adjusted for age, sex, and weight status. Significant two-tailed tests were set at p < .05.

Results
Of the four musculoskeletal fitness measures, only 90o push-up was significantly associated (β = .353; p < .001) with PACER test scores (i.e., cardiorespiratory fitness). The related model (R2 = .324; F (4,205) = 26.061; p < .001) accounted for 32% of the variance in cardiorespiratory fitness. 90o push-up was associated with sit-and reach (β = .298; p < .001) and curl up (β = .413; p < = .001) test scores. When individually modeled, 90o push-up (β = −.461; p < .001) and PACER (β = −.436; p < .001) were inversely associated with weight status.

Conclusions
The 90o push-up test (a measure of upper body muscle strength and endurance) was associated with cardiorespiratory fitness, anterior trunk muscle strength and endurance, and lower back and posterior thigh muscle flexibility in youth aged 8–12 years old. Although the current findings do not establish a causal relationship, it is concluded that the 90o push-up test is a tractable tool for physical fitness surveillance by clinicians, parents, and possibly youth themselves.