bluuebunny/crossref_metadata_2025_split huggingface dataset:
Concomitant singularities of Yb-valence and magnetism at a critical lattice parameter of icosahedral quasicrystals and approximants
Authors: Imura Keiichiro , Yamaoka Hitoshi , Yokota Shinjirou , Sakamoto Kazushi , Yamamoto Yoshiya , Kawai Takuma , Namba Keisuke , Hirokawa Shinnosuke , Deguchi Kazuhiko , Hiraoka Nozomu , Ishii Hirofumi , Mizuki Jun’ichiro , Ishimasa Tsutomu , Sato Noriaki K. 
Published: October 2020

AbstractNon-Fermi-liquid (NFL), a significant deviation from Fermi-liquid theory, usually emerges near an order-disorder phase transition at absolute zero. Recently, a diverging susceptibility toward zero temperature was observed in a quasicrystal (QC). Since an electronic long-range ordering is normally absent in QCs, this anomalous behaviour should be a new type of NFL. Here we study high-resolution partial-fluorescence-yield x-ray absorption spectroscopy on Yb-based intermediate-valence icosahedral QCs and cubic approximant crystals (ACs), some of which are new materials, to unveil the mechanism of the NFL. We find that for both forms of QCs and ACs, there is a critical lattice parameter where Yb-valence and magnetism concomitantly exhibit singularities, suggesting a critical-valence-fluctuation-induced NFL. The present result provides an intriguing structure–property relationship of matter; size of a Tsai-type cluster (that is a common local structure to both forms) tunes the NFL whereas translational symmetry (that is present in ACs but absent in QCs) determines the nature of the NFL against the external/chemical pressure.
Dystrophin Dp71ab is monoclonally expressed in human satellite cells and enhances proliferation of myoblast cells
Authors: Farea Manal , Rani Abdul Qawee Mahyoob , Maeta Kazuhiro , Nishio Hisahide , Matsuo Masafumi 
Published: October 2020

AbstractDystrophin Dp71 is the smallest isoform of the DMD gene, mutations in which cause Duchenne muscular dystrophy (DMD). Dp71 has also been shown to have roles in various cellular processes. Stem cell-based therapy may be effective in treating DMD, but the inability to generate a sufficient number of stem cells remains a significant obstacle. Although Dp71 is comprised of many variants, Dp71 in satellite cells has not yet been studied. Here, the full-length Dp71 consisting of 18 exons from exons G1 to 79 was amplified by reverse transcription-PCR from total RNA of human satellite cells. The amplified product showed deletion of both exons 71 and 78 in all sequenced clones, indicating monoclonal expression of Dp71ab. Western blotting of the satellite cell lysate showed a band corresponding to over-expressed Dp71ab. Transfection of a plasmid expressing Dp71ab into human myoblasts significantly enhanced cell proliferation when compared to the cells transfected with the mock plasmid. However, transfection of the Dp71 expression plasmid encoding all 18 exons did not enhance myoblast proliferation. These findings indicated that Dp71ab, but not Dp71, is a molecular enhancer of myoblast proliferation and that transfection with Dp71ab may generate a high yield of stem cells for DMD treatment.
Glucose-6-phosphate dehydrogenase deficiency in the Han Chinese population: molecular characterization and genotype–phenotype association throughout an activity distribution
Authors: He Ying , Zhang Yinhui , Chen Xionghao , Wang Qiong , Ling Lifen , Xu Yuhong 
Published: October 2020

AbstractGlucose-6-phosphate dehydrogenase (G6PD) deficiency is a common hereditary disorder in China. The existing prevalence and molecular epidemiology of G6PD deficiency in China were geographically limited. In this study, the spectrum of G6PD gene mutations was well characterized in a large and diverse population all over the country; and the correlation of genotype and enzyme activity phenotype was explored for the first time. The results showed that the overall prevalence of G6PD deficiency in China was 2.10% at the national level. The top six common mutations were c.1388 G>A, c.1376 G>T, c.95 A>G, c.392 G>T, c.871 G>A and c.1024 C>T, accounting for more than 90% of G6PD deficient alleles. Compound mutation patterns were frequently observed in females with severe deficiency. The distribution of G6PD activities depended on the type of mutation patterns and genders. Hemizygote, homozygote, and compound heterozygote were predominantly associated with severe G6PD deficiency, whereas heterozygotes with single mutation mainly presented moderate enzyme deficiency. A significant gap between G6PD activities in hemizygous and normal males was observed, and yet, the overall distribution of that in females carrying missense mutations was a continuum from G6PD severely deficient to normal. This is the first report of discussing the association between G6PD genetic variants in the Chinese and enzyme activity phenotypes.
Dielectric tetrahedrons as terahertz resonators switched from perfect absorber to reflector
Authors: Chen Haosheng , Zhou Chenchen , Li Yongjian , Liang Shuaishuai , Li Jiang 
Published: October 2020

AbstractTetrahedrons are basic building blocks in natural and artificial materials, while the terahertz response of micro tetrahedrons has been little explored. Here we fabricate subwavelength ceramic tetrahedrons for use in the terahertz frequency range, and find that the three-dimensional geometry significantly affects their terahertz properties. The transmission spectra are independent of the orientation of the tetrahedrons, while the first magnetic resonance disappears in the reflection spectra when an upright tetrahedron is flipped upside down on the metallic substrate, which changes it from a perfect absorber to a perfect reflector. This is attributed to the destructive interference between two magnetic dipoles induced respectively by the incident and the reflected wave. The study brings new insights in the materials design with 3D building blocks to realize more interesting and exotic terahertz properties.
eQTL mapping of the 12S globulin cruciferin gene PGCRURSE5 as a novel candidate associated with starch content in potato tubers
Authors: Sołtys-Kalina Dorota , Szajko Katarzyna , Stefańczyk Emil , Smyda-Dajmund Paulina , Śliwka Jadwiga , Marczewski Waldemar 
Published: October 2020

AbstractTuber starch content (TSC) is a very important trait in potato(Solanum tuberosumL.). This study is the first to use expression quantitative trait loci (eQTL) mapping of transcript-derived markers for TSC in potato. Thirty-four differentially expressed genes were selected by comparing the RNA-seq data of contrasting bulked segregants. For the 11 candidate genes, we determined their relative expression levels across the segregating diploid potato population using RT-qPCR. We detected 36 eQTL as candidate genes distributed on all twelve potato chromosomes, and nine of them overlapped with QTL for TSC. Peaks for two eQTL, eAGPaseS-a and ePGRCRURSE5, were close to the corresponding loci of the large subunit of ADP-glucose pyrophosphorylase (AGPaseS-a) and the 12S globulin cruciferin gene (PGCRURSE5), respectively. The eQTL peaks forAGPaseS-aandPGRCRURSE5explained 41.0 and 28.3% of the phenotypic variation at the transcript level. We showed the association of the DNA markers forAGPaseS-aandPGRCRURSE5with QTL for TSC, and significant correlation between the expression level ofPGRCRURSE5and TSC. We did not observe a significant correlation between the expression level ofAGPaseS-aand TSC. We concluded that the cruciferin genePGRCRURSE5is a novel candidate involved in the regulation of starch content in potato tubers.
MHC/class-II-positive cells inhibit corticosterone of adrenal gland cells in experimental arthritis: a role for IL-1β, IL-18, and the inflammasome
Authors: Stangl Hubert , Krammetsvogl Anita , Lesiak Martin , Wolff Christine , Straub Rainer H. 
Published: October 2020

AbstractIn experimental arthritis, glucocorticoid secretion is inadequate relative to inflammation. We hypothesized that IL-1 is a key factor for inadequate glucocorticoid secretion in arthritic rats. Collagen type II—induced arthritis (CIA) in DA rats was the model to study effects of IL-1 on adrenal function. In the CIA model, an increase of intraadrenal MHCII-positive cells was observed. MHCII-positive cells or bone marrow-derived dendritic cells inhibited glucocorticoid secretion of adrenal gland cells. IL-1, but also IL-18 and the inflammasome were critical in glucocorticoid inhibition. Arthritic compared to control adrenal gland cells produced higher amounts of CXC chemokines from MHCII+ adrenal cells, particularly CINC-2, which is strongly dependent on presence of IL-1. In CIA, macrophages and/or dendritic cells inhibit glucocorticoid secretion via IL-1 in adrenal glands. These findings show that activated macrophages and/or dendritic cells inhibit glucocorticoid secretion in experimental arthritis and that IL-1β is a decisive factor.
Lack of tocilizumab effect on mortality in COVID19 patients
Authors: Holt Gregory E. , Batra Mayank , Murthi Mukunthan , Kambali Shweta , Santos Kayo , Bastidas Maria Virginia Perez , Asif Huda , Haddadi Sara , Arias Sixto , Mirsaeidi Mehdi 
Published: October 2020

AbstractOff-label tocilizumab use in COVID-19 patients reflects concern for cytokine release syndrome. Comparison of matched COVID-19 pneumonia patients found elevated IL-6 levels correlated with mortality that did not change with tocilizumab administration. Correlating mortality with increased IL-6 doesn’t imply causality however lack of improvement by tocilizumab requires further clinical trial alterations.
Ultra stable, inkjet-printed pseudo reference electrodes for lab-on-chip integrated electrochemical biosensors
Authors: Papamatthaiou Sotirios , Zupancic Uros , Kalha Curran , Regoutz Anna , Estrela Pedro , Moschou Despina 
Published: October 2020

AbstractLab-on-Chip technology comprises one of the most promising technologies enabling the widespread adoption of Point-of-Care testing in routine clinical practice. However, until now advances in Lab-on-Chip have not been translated to the anticipated degree to commercialized tools, with integrated device mass manufacturing cost still not at a competitive level for several key clinical applications. Lab-on-PCB is currently considered as a candidate technology addressing this issue, owing to its intuitive compatibility with electronics, seamless integration of electrochemical biosensors and the extensive experience regarding industrial manufacturing processes. Inkjet-printing in particular is a compatible fabrication method, widening the range of electronic materials available and thus enabling seamlessly integrated ultrasensitive electronic detection. To this end, in this work stable pseudo-reference electrodes are fabricated for the first time by means of commercial inkjet-printing on a PCB-integrated electrochemical biosensing platform. SEM and XPS analysis are employed to characterize the electrodes’ structure and composition and identify any special characteristics, compared to published work on alternative substrates. Additionally, this paper analyzes integrated reference electrodes from a new perspective, focusing mainly on their characteristics in real-life operation: chemical sintering as opposed to high budget thermal one, stability under continuous flow, pH dependency and bias stress effects on electrode instability, a parameter often overlooked in electrochemical biosensors.
Thermal expansion and dimensionality of a hydrogen bond network: a case study on dimorphic oxalic acid
Authors: Bhattacharya Suman 
Published: October 2020

Between 156 K–298 K the oxalic acid polymorphs, α-C2 and β-C2 exhibit comparable volumetric thermal expansions which are correlated to the respective crystal packing and intermolecular interactions in the two forms.
Detection of genes responsible for cetuximab sensitization in colorectal cancer cells using CRISPR-Cas9
Authors: Hu Ting-ting , Yang Jia-wen , Yan Ye , Chen Ying-ying , Xue Hai-bo , Xiang You-qun , Ye Le-chi 
Published: October 2020

Abstract
Colorectal cancer (CRC) is a common malignant tumor in digestive tract with highly invasive and metastatic capacity. Drug sensitivity remains a significant obstacle to successful chemotherapy in CRC patients. The present study aimed to explore genes related to cetuximab (CTX) sensitivity in CRC by clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9. Celigo image cytometer was used to detect suitable cells and optimal dosage of CTX. Inhibition rate of CTX on Caco-2 cells was evaluated by cell counting kit-8 (CCK-8) method before and after transfection. 3-(4,5-dimethylthiazol-2-yl)2,5-diphenyl tetrazolium bromide (MTT) was performed to explore suitable concentration of puromycin and multiplicity of infection (MOI). CRISPR-Cas9, sequencing data quality analysis and cell viability test were used for the selection of genes related to CTX sensitivity in CRC cells. Finally, the selected genes associated with CTX sensitivity in CRC cells were further validated by colony formation and CCK-8 assays. In the present study, Caco-2 cells had a better prolificacy, and CTX 100 μg/ml exhibited a good inhibition trend on the 7th and 14th days of infection. MTT assay indicated that the minimum lethal concentration of puromycin was 2.5 μg/ml. Forty-six candidate genes were preliminarily screened via sequencing data quality analysis. Subsequently, we found that knockout of any of the four genes (MMP15, MRPL48, CALN1 and HADHB) could enhance CTX sensitivity in Caco-2 cells, which was further confirmed by colony formation assay. In summary, MMP15, MRPL48, CALN1 and HADHB genes are related to the mediation of CTX sensitivity in CRC.
Creation and Initial Validation of the Caregiver Analysis of Reported Experiences with Swallowing Disorders (CARES) Screening Tool
Authors: Shune Samantha E. , Resnick Barbara , Zarit Steven H. , Namasivayam-MacDonald Ashwini M. 
Published: October 2020

Purpose
Dysphagia is a debilitating condition with widespread consequences. Previous research has revealed dysphagia to be an independent predictor of caregiver burden. However, there is currently no systematic method of screening for or identifying dysphagia-related caregiver burden. The aim of this study was to develop a set of questions for a dysphagia-related caregiver burden screening tool, the Caregiver Analysis of Reported Experiences with Swallowing Disorders (CARES), and pilot the tool to establish preliminary validity and reliability.

Method
The questionnaire was developed through an iterative process by a team of clinical researchers with expertise in dysphagia, dysphagia-related and general caregiver burden, and questionnaire design. A heterogenous group of 26 family caregivers of people with dysphagia completed the CARES, along with the Eating Assessment Tool (EAT-10), the International Dysphagia Diet Standardisation Initiative Functional Diet Scale (IDDSI-FDS), and the Zarit Burden Interview (ZBI). Information on construct validity, item fit, convergent validity, internal consistency, and reliability was determined via Rasch analysis model testing, Cronbach's alpha, and Spearman's rho calculations.

Results
The final CARES questionnaire contained 26 items divided across two subscales. The majority of the questionnaire items fit the model, there was evidence of internal consistency across both subscales, and there were significant relationships between dysphagia-specific burden (CARES) and perceived swallowing impairment (EAT-10), general caregiver burden (ZBI), and diet restrictiveness (IDDSI-FDS).

Conclusions
Results from the current study provide initial support for the validity and reliability of the CARES as a screening tool for dysphagia-related burden, particularly among caregivers of adults with swallowing difficulties. While continued testing is needed across larger groups of specific patient populations, it is clear that the CARES can initiate structured conversations about dysphagia-related caregiver burden by identifying potential sources of stress and/or contention. This will allow clinicians to then identify concrete methods of reducing burden and make appropriate referrals, ultimately improving patient care.
Development and Validation of Sentences Without Semantic Context to Complement the Basic English Lexicon Sentences
Authors: O'Neill Erin R. , Parke Morgan N. , Kreft Heather A. , Oxenham Andrew J. 
Published: October 2020

Purpose
The goal of this study was to develop and validate a new corpus of sentences without semantic context to facilitate research aimed at isolating the effects of semantic context in speech perception.

Method
The newly developed corpus contains nonsensical sentences but is matched in vocabulary and syntactic structure to the existing Basic English Lexicon (BEL) corpus. It consists of 20 lists, with each list containing 25 sentences and each sentence having four keywords. Each new list contains the same keywords as the respective list in the original BEL corpus, but the keywords within each list are scrambled across sentences to eliminate semantic context within each sentence, while maintaining the original syntactic structure. All sentences in the original and nonsense BEL corpora were recorded by the same two male and two female talkers.

Results
Mean intelligibility scores for each list were estimated by calculating the mean proportion of correct keywords achieved by 40 normal-hearing listeners for one male and one female talker. Although small but significant differences were found between some pairs of lists, mean performance for all 20 lists fell within the 95% confidence intervals of the mean.

Conclusions
Lists in the newly developed nonsense corpus are reasonably well equated for difficulty and can be used interchangeably in a randomized experimental design. Both the original and nonsense BEL sentences, all recorded by the same four talkers, are publicly available.

Supplemental Material

https://doi.org/10.23641/asha.13022900
Ageism Among Graduate Students in Communication Sciences and Disorders: A Longitudinal Analysis
Authors: Heape Amber , Causey Brandon , Lloyd Taylor , Jeter Stevonna 
Published: October 2020

Purpose
Ageism is a negative bias that results in stereotyping of and discrimination against older individuals and is one of the most institutionalized forms of prejudice in current society. Literature suggests that negative attitudes toward the elderly may have adverse effects, especially in health care settings. The purpose of this investigation was to examine the presence and level of ageism in a group of graduate students enrolled in an accredited speech-language pathology program over a 2-year period, to determine if the degree of ageism differed based on ethnicity, gender, age, or previous experience working with older adults.

Method
Eighty students of an accredited speech-language pathology graduate program completed the Fraboni Scale of Ageism over a 2-year period at the onset of a course on aging. Students were blinded to the type of assessment they were completing and were anonymous in order to blind the instructor to specific student performance. Demographic data for each protocol were gathered in order to analyze results.

Results
Data were analyzed using descriptive statistics and analysis of variance calculated for effect size between groups. Results indicated that the level of ageism did not significantly change when grouped by gender, ethnicity, age, or level of experience, though a level of implicit bias was demonstrated through overall participant scores.

Conclusions
Authors concluded that existing evidence should be used to develop graduate curriculum that encompasses all age groups, especially those aged 65 years and older. Opportunities for experiential learning may provide further benefit to decreasing ageism in graduate students, leading to positive societal change.
The magnetic fine structure of the Sun’s polar region as revealed by Sunrise
Authors: Prabhu A. , Lagg A. , Hirzberger J. , Solanki S. K. 
Published: October 2020

Context. Polar magnetic fields play a key role in the solar magnetic cycle and they are the source of a significant portion of the interplanetary magnetic field. However, observations of the poles are challenging and hence our understanding of the polar magnetic environment is incomplete.
Aims. We deduce properties of small-scale magnetic features in the polar region using high-resolution data and specifically aim to determine the flux per patch above which one magnetic polarity starts to dominate over the other.
Methods. We study the high spatial resolution, seeing-free observations of the north solar polar region, obtained with the IMaX instrument on-board the balloon-borne SUNRISE observatory during June 2009, at the solar activity minimum. We performed inversions of the full Stokes vector recorded by IMaX to retrieve atmospheric parameters of the Sun’s polar region, mainly the temperature stratification and the magnetic field vector.
Results. We infer kilo-Gauss (kG) magnetic fields in patches harbouring polar faculae, without resorting to a magnetic filling factor. Within these patches we find the maxima of the magnetic field to be near the dark narrow lanes, which are shifted towards the disc centre side in comparison to the maxima in continuum intensity. In contrast, we did not find any fields parallel to the solar surface with kG strengths. In addition to the kG patches, we found the polar region to be covered in patches of both polarities, which have a range of sizes. We find the field strength of these patches to increase with increasing size and flux, with the smaller patches showing a significant dispersion in field strength. The dominating polarity of the north pole during this phase of the solar cycle is found to be maintained by the larger patches with fluxes above 2.3  ×  1017 Mx.
Praxis der Sterbehilfe durch Ärzte und Pflegekräfte in deutschen Krankenhäusern
Authors: Beine Karl H. 
Published: October 2020

Zusammenfassung
Hintergrund Aktive Sterbehilfe und ärztlich assistierter Suizid werden in Deutschland kontrovers diskutiert. Empirische Studien fehlen, um ihr jeweiliges Vorkommen, einschließlich dem von passiver und indirekter Sterbehilfe, in deutschen Krankenhäusern einschätzen zu können. Ärztinnen und Ärzte sowie Pflegerinnen und Pfleger in deutschen Krankhäusern wurden zu ihrer Anwendung von Sterbehilfe befragt und mögliche Einflussfaktoren erhoben.
Methode Angaben zu Sterbehilfe wurden mit deskriptiven Begriffen und zugeordneten Definitionen in einer anonymen Online-Befragung erhoben. Die objektive und subjektive Arbeitssituation der Befragten und ihre Befürwortung von aktiver Sterbehilfe wurden erfasst. Die finale ärztliche Stichprobe umfasste n = 2507, die pflegerische Stichprobe umfasste n = 2683 Personen.
Ergebnis Die Anwendung von passiver und indirekter Sterbehilfe innerhalb von 24 Monaten wurde von einem großen Anteil der ärztlichen und pflegerischen Befragten angegeben, aktive Sterbehilfe und assistierter Suizid von deutlich weniger Befragten. Die Varianz in der Anwendung von aktiver Sterbehilfe wurde u. a. durch mehrere arbeitsbezogene Faktoren und die jeweilige Befürwortung von aktiver Sterbehilfe beeinflusst, nicht aber durch subjektive Belastungsfaktoren.
Schlussfolgerung Sterbehilfe wird durch ärztliche und pflegerische Mitarbeiterinnen und Mitarbeiter in deutschen Krankenhäusern praktiziert. Das Vorkommen unterschiedlicher Formen von Sterbehilfe und relevante Einflussfaktoren werden vor dem Hintergrund methodischer Limitationen diskutiert.