bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification and validation of the diagnostic biomarker MFAP5 for CAVD with type 2 diabetes by bioinformatics analysis
Authors: Shen Qiang , Fan Lin , Jiang Chen , Yao Dingyi , Qian Xingyu , Tong Fuqiang , Fan Zhengfeng , Liu Zongtao , Dong Nianguo , Zhang Chao , Shi Jiawei 
Published: December 2024

IntroductionCalcific aortic valve disease (CAVD) is increasingly prevalent among the aging population, and there is a notable lack of drug therapies. Consequently, identifying novel drug targets will be of utmost importance. Given that type 2 diabetes is an important risk factor for CAVD, we identified key genes associated with diabetes - related CAVD via various bioinformatics methods, which provide further potential molecular targets for CAVD with diabetes.MethodsThree transcriptome datasets related to CAVD and two related to diabetes were retrieved from the Gene Expression Omnibus (GEO) database. To distinguish key genes, differential expression analysis with the “Limma” package and WGCNA was applied. Machine learning (ML) algorithms were employed to screen potential biomarkers. The receiver operating characteristic curve (ROC) and nomogram were then constructed. The CIBERSORT algorithm was utilized to investigate immune cell infiltration in CAVD. Lastly, the association between the hub genes and 22 types of infiltrating immune cells was evaluated.ResultsBy intersecting the results of the “Limma” and WGCNA analyses, 727 and 190 CAVD - related genes identified from the GSE76717 and GSE153555 datasets were obtained. Then, through differential analysis and interaction, 619 genes shared by the two diabetes mellitus datasets were acquired. Next, we intersected the differential genes and module genes of CAVD with the differential genes of diabetes, and the obtained genes were used for subsequent analysis. ML algorithms and the PPI network yielded a total of 12 genes, 10 of which showed a higher diagnostic value. Immune cell infiltration analysis revealed that immune dysregulation was closely linked to CAVD progression. Experimentally, we have verified the gene expression differences of MFAP5, which has the potential to serve as a diagnostic biomarker for CAVD.ConclusionIn this study, a multi-omics approach was used to identify 10 CAVD-related biomarkers (COL5A1, COL5A2, THBS2, MFAP5, BTG2, COL1A1, COL1A2, MXRA5, LUM, CD34) and to develop an exploratory risk model. Western blot (WB) and immunofluorescence experiments revealed that MFAP5 plays a crucial role in the progression of CAVD in the context of diabetes, offering new insights into the disease mechanism.
Photobiomodulation therapy for congenital color vision deficiency: results of a preliminary randomized clinical trial
Authors: Liu Yidi , Yang Qinghua , Yu Jun , Jia Liang 
Published: December 2024

PurposeThis study presents a novel randomized controlled trial investigating photobiomodulation (PBM) therapy as an intervention method for color vision deficiency (CVD).MethodsA total of 74 participants with CVD were assigned to either the PBM group or the control group. In the PBM group, participants wore virtual reality (VR) goggles twice daily, with a 12-h interval, over a four-week period. The VR video consisted of alternating red and green images, each presented for 5 s, totaling 6 min and 20 s. No treatment was administered to the control group. Color vision improvement was assessed using Yu’s, Ishihara’s pseudoachromatic plates, Color Blindness Check (CBC), and FM-100 Hue total error score (TES).ResultsAfter 4 weeks, in terms of Yu’s and Ishihara’s Plates, the patients in PBM group could identify increasing pieces (before: 1.6 ± 1.6, 2.3 ± 2.2; 4 weeks: 6.5 ± 4.4, 5.4 ± 2.9), while in control group, the number was before: 2.6 ± 3.4, 2.6 ± 2.5; 4 weeks: 3.3 ± 3.6, 2.9 ± 2.2. As for CBC scores, the patients in PBM also showed improved high scores (before: 2353.3 ± 700.0; 4 weeks: 2693.6 ± 642.5). Moreover, PBM treatment resulted in a significant reduction of FM-100 scores (before: 298.0 ± 211.3; 4 weeks: 202.1 ± 114.4).ConclusionThese findings suggest that PBM therapy holds promise as a potential treatment option for individuals with CVD.Clinical trial registrationThe study received approval from the Ethics Committee of PLA General Hospital, China (KY2021-017). Additionally, it was registered as a Chinese domestic clinical trial (ChiCTR2200056761) at “http://Chictr.org.cn/index.aspx”.
Efficiency of a non-recycling postharvest fungicide drencher to enhance management of apple decay and food safety
Authors: Amiri Achour , Murphy Claire M. , Hoheisel Gween A. , Haskell Clayton L. , Critzer Faith 
Published: December 2024

IntroductionRecycling drenchers used to apply postharvest fungicides in pome fruit may spread microorganisms, i.e., plant and foodborne pathogens, that increase fruit loss and impact food safety.MethodsA non-recycling field drencher (FD), which drenches unstacked bins of fruit, was compared to a commercial recycling packinghouse drencher (CPD) for fruit coverage, fungicide residues, postharvest diseases control and spread of plant pathogens, total coliforms and generic Escherichia coli. A mixture of fludioxonil (FDL) and thiabendazole (TBZ) was used in 2021, while pyrimethanil (PYR) was applied in 2022 to alternate fungicides.ResultsThe overall spray coverage assessed with pyranine was not significantly different between the FD and CPD. The residue levels of FDL and TBZ were similar between the two methods on Honeycrisp apples at the top, middle, and the bottom of the bins, whereas the residue levels of PYR were significantly lower at the bottom of the bins treated through the FD. The density of plant pathogens and overall disease incidence were similar on apples drenched through both systems in 2021 and significantly lower in FD-treated apples in 2022. The incidence of blue mold, the most important postharvest disease caused by Penicillium spp., was significantly lower in apples treated through the FD in both years. The levels of total coliforms and generic E. coli were significantly higher in fungicide solutions collected from the CPD compared to the FD. Total coliforms increased significantly on apples treated via the CPD but not on apples treated through the FD.DiscussionFindings from this study suggest that the new non-recycling drencher has potential as an alternative to recycling packinghouse drenchers in reducing the spread of plant and foodborne pathogens.
Effects of butylphthalide sodium chloride injection combined with edaravone dexborneol on neurological function and serum inflammatory factor levels in sufferers having acute progressive cerebral infarction
Authors: Li Yaqiang , Liu Jie , Zhu Yuanyuan , Xue Min , He Jiale 
Published: December 2024

ObjectivesFor investigating an influence on butylphthalide sodium chloride injection combined with edaravone dextromethorphan on neurological function, serum free radical levels, and serum inflammatory factor levels in sufferers having acute progressive cerebral infarction (APCI).MethodsA cohort of 200 patients, afflicted by APCI and admitted to our healthcare institution between December 2018 through July 2023, were incorporated into this longitudinal prospective analysis. Employing a random number table methodology, the patient cohort was bifurcated into a control group (n = 100) and an observation group (n = 100). The control group is treated with butylphthalide sodium chloride injection and the study group is treated with edaravone dexborneol, based on the control group. The posttreatment curative efficacy on the two groups is recorded, and treatment of both the two groups is compared. Before and after neurological function indexes (NIHSS and BI), inflammatory factor indexes (hs-CRP, IL-8, and TNF-a), serum free radical levels (ROS, NO, and SOD), and contrasted between the two groups of treatment effect during therapy.ResultsCompared with the control (65%), the observation group exhibited a significantly higher effective rate of around 89%. In the observation group, the improvement in NIHSS, and Barthel index scores; inflammatory indexes including hs-CRP, IL-8, and TNF-a; and serum free radical levels including ROS, NO, and SOD in peripheral blood was better than the control group, with significant difference (all P&lt;0.05).ConclusionThe clinical efficacy of edaravone dexborneol combined with butylphthalide sodium chloride injection in the treatment of acute progressive cerebral infarction is precise, which can effectively reduce the level of inflammatory factors and serum free radicals in patients, improve the neurological function of patients, and reduce the damage of cerebral cells, and the safety is high.
Subtemporal approach for posterior communicating artery aneurysms
Authors: Lan Jing , Huang Xiao , Liu Yue , Zhang Ting-bao , Chen Jin-cao , Li Zheng-wei 
Published: December 2024

Background and objectivesDirect visualization of the aneurysmal neck and its related perforating arteries during microsurgical clipping of posterior communicating artery (PCoA) aneurysms with posterior projection or true PCoA aneurysms through the pterional approach may be difficult and complicated.MethodsFrom January 2022 to January 2023, the clinical and angiographic information regarding PCoA aneurysms were retrospectively collected. Among them, 10 consecutive patients with PCoA aneurysms treated with microsurgical clipping via the subtemporal approach in our single institution were included. Herein, we analyzed and summarized our experience and clinical outcomes to further evaluate the efficacy, safety and feasibility of this approach as well as the indications.ResultsAll aneurysms were completely clipped via the subtemporal approach. With respect to procedure-related complications, postoperative oculomotor nerve palsy occurred in one patient (10%), contralateral cerebral infarction in one patient (10%), and intraoperative rupture of the aneurysm in three patients (30%). There were no cases of temporal lobe contusion or venous injury in this group. Overall, Good outcomes were obtained in 9 patients (90%), and poor functional outcome was observed in 1 patient (10%) at the last follow-up.ConclusionThe management of true PCoA aneurysms and PCoA aneurysms projecting posteriorly is more complicated and challenging, and treating these lesions entails considerable risks via the pterional approach. Considering the above problems, we attempted to treat these refractory PCoA aneurysms through the subtemporal approach. Our results suggested that microsurgical clipping of these aneurysms via the subtemporal approach can achieve good clinical outcomes with a high preservation rate of the related branches. Appropriate patient selection, fully grasp of indications, precise understanding of the anatomy and thorough preoperative planning are crucial for successful surgery. The subtemporal approach appears to be a relatively safe and effective procedure in the experienced hands, and may be an alternative method for patients with true PCoA aneurysms or projecting posteriorly PCoA aneurysms which cannot be easily clipped from the pterional approach. It can provide a better lateral view to visualize the neck of the aneurysm, PCoA and its perforating vessels, as well as the other structures.
Galvanic vestibular stimulation for the postural rehabilitation of HTLV-1-associated myelopathy
Authors: Silva Tatiana Rocha , Labanca Ludimila , Caporali Júlia Fonseca de Morais , Tavares Mauricio Campelo , Rausse Nathália de Castro Botini , de Almeida Maria Júlia Amaral Abranches , Martins Maxmilliam de Souza , Amorim Laura Fernandes , Sitibaldi Léo Dantas , Gonçalves Denise Utsch 
Published: December 2024

IntroductionGalvanic vestibular stimulation (GVS) is a simple, safe, and noninvasive method of neurostimulation that can be used to improve body balance. Several central nervous system diseases cause alterations in body balance, including HTLV-1-associated myelopathy (HAM).ObjectiveTo test GVS as a balance rehabilitation strategy for HAM.MethodsThis study is a quasi-experimental clinical trial in which postural balance was compared before and after a GVS rehabilitation protocol applied to 20 patients with HAM, 12 women and 8 men, average age of 78 and 79 years, respectively. They were followed for nine months after the end of the GVS protocol, which consisted of one GVS session per week for 12 consecutive weeks. The GVS current intensity was progressively increased from 1.0 milliamperes (mA) to 3.5 mA until the third session and maintained at 3.5 mA until the 12th session. The electrical stimulation time progressively increased from 9 min in the first session to 18 min in the second session and maintained at 30 min from the third session onwards. Postural balance was assessed by Time up and go test (TUG), Berg balance scale (BBS) and posturography that were performed before the beginning of the intervention, during the intervention (6th week), at the end of the intervention (12th week) and after 9 months of follow-up without electrical stimulation.ResultsIn a blind comparison, in the 12th week of stimulation, improvement was observed in all the tests. In TUG, time in seconds changed from 28 before to 18 after GVS (p &lt; 0,001). In BBS, the score changed from 29.00 before to 41.00 points after GVS. In posturography, the stability limit improved after the intervention (p &lt; 0.05). However, after nine months without stimulation, the gain was lost for TUG, for BBS and for stability limit.ConclusionGVS was an effective method to improve postural instability of patients with HAM in the short term, but the gain in postural stability was not maintained in the long term. A device for home use may be an option for long-term use.
Oxidative balance score is associated with the risk of diabetic kidney disease in patients with type 2 diabetes mellitus: evidence from NHANES 2007–2018
Authors: Liang Yu , Xu Zhonggao , Wang Wanning 
Published: December 2024

ObjectiveThe oxidative balance score (OBS) is a comprehensive measure of oxidative stress that is calculated from the combined prooxidant and antioxidant scores of 16 dietary components and four lifestyle factors. This study aimed to evaluate the relationship between OBS and the risk of diabetic kidney disease (DKD) in individuals with Type 2 diabetes mellitus (T2DM).MethodsData were obtained from the NHANES. A cross-sectional study was conducted using multiple logistic regression. Covariate effects of this relationship were also examined using subgroup analysis.ResultsWe evaluated 3,669 T2DM participants, among whom DKD prevalence was 30.87%. In a fully adjusted logistic regression model, the risk of DKD among participants with OBS, lifestyle OBS, and dietary OBS in the highest quartile group was 0.50 times (95% CI: 0.39 to 0.65), 0.54 times (95% CI: 0.41–0.71), and 0.45 times (95% CI: 0.32–0.63), respectively, than that in the lowest quartile group, respectively. In addition, participants who scored in the top quartiles of OBS were more likely to possess higher levels of education and income. A stratified analysis demonstrated the robustness of these findings.ConclusionOBS negatively correlates with the risk of DKD among individuals with T2DM.
Association between serum β-carotene and suicidal ideation in adults: a cross-sectional study
Authors: Lv Jihua , Xu Tong , Lou Shuyue , Zhan Zhenxiang , Cheng Zicheng , Fu Fangwang 
Published: December 2024

BackgroundThe aim was to ascertain whether serum carotenoid levels are linked to suicidal ideation, along with how depressive disorder influences the association.MethodsThis research was conducted using a cross-sectional design and encompassed 7,335 adults from the United States. The levels of serum carotenoids, encompassing α-carotene, β-carotene, β-cryptoxanthin, lycopene, and lutein/zeaxanthin, were ascertained by employing high-performance liquid chromatography. Item nine of the nine-item Patient Health Questionnaire was used to evaluate suicidal ideation. The study used multivariable-adjusted logistic regression and restricted cubic spline models to ascertain the link between serum carotenoid levels and suicidal ideation. Mediation and stratified analyses were used to ascertain the impact of depressed symptoms on the association between serum carotenoid levels and suicidal ideation.ResultsOut of the total participants, 245 individuals (3.3%) reported having suicidal ideation. Participants who had suicidal ideation showed lower levels of serum α-carotene, β-carotene, β-cryptoxanthin, and lutein/zeaxanthin compared to those who did not have suicidal ideation. After controlling potential confounding factors, serum β-carotene level was still associated with risk of suicidal ideation (per 1–standard deviation (SD) increment, odds ratio [OR]: 0.73, 95% confidence interval [CI]: 0.55–0.98). The mediation analysis revealed that 36.3% of this association was mediated by the severity of depressive symptoms. Stratified analysis manifested that the association remained in depressed people but was attenuated in people without depressive disorder.ConclusionIncreased serum β-carotene level may decrease the susceptibility to suicidal ideation, especially in depressed people. Further intervention studies are needed to validate whether β-carotene consumption contributes to preventing suicidal ideation.
Case report: Anesthesia management for surgical treatment of glucagonoma with symptom of characterized necrolytic migratory erythema
Authors: Xia Di , Shen Le 
Published: December 2024

BackgroundThe anesthetic management of patients with glucagonoma is complicated by a number of factors including glucose fluctuation, characterized necrolytic migratory erythema in oral and pharyngeal, which may lead to an unexpected difficult airway.Case presentationHerein we describe the anesthetic considerations and management of a 47-year-old adult with glucagonoma, who presented for a laparoscopic splenectomy and distal pancreatectomy procedure.ConclusionThis report details fiberoptic intubation in an adult with glucagonoma and necrolytic migratory erythema. We recommend that this approach be considered in patients with glucagonoma and severe necrolytic migratory erythema undergoing general anesthesia.
Open-ended urethral catheters reduce catheter obstruction after hypospadias repair
Authors: Zhao Xiang , Yu Kechi , Fang Erhu , Li Ning 
Published: December 2024

BackgroundsUrethral catheter obstruction is a notable issue that pediatric patients with hypospadias may encounter in the early postoperative period. This retrospective study aims to assess the efficacy of open-ended urethral catheters with 2 side holes in mitigating catheter obstruction in pediatric patients following hypospadias repair.Materials and methodsThe clinical data of pediatric patients who underwent hypospadias repair surgery from January 2021 to October 2023 were retrospectively collected. The patients were divided into 2 groups. Those who used standard Foley catheters were referred to as Group A, while those who used modified open-ended catheters were referred to as Group B. The primary outcome was the incidence of catheter obstruction within 7 days postoperatively.ResultsA total of 297 patients were included in this study, with 142 patients in Group A and 155 patients in Group B. In Group A, there were 12 cases of catheter obstruction, with 10 cases resolved through irrigation and 2 cases requiring additional catheters insertion by suprapubic bladder punctures. In Group B, only 4 cases experienced catheter obstruction, which were effectively and easily resolved through maneuver irrigation. The incidence of catheter obstruction in Group B was statistically significantly lower than that in Group A (2.6% vs. 8.5%, p &lt; 0.05).ConclusionIn pediatric hypospadias patients who underwent surgical repair, the use of open-ended urethral catheters with 2 side holes significantly diminishes the incidence of postoperative catheter obstruction. This simple technique is worthy of promotion.
DCP-YOLOv7x: improved pest detection method for low-quality cotton image
Authors: Ma Yukun , Wei Yajun , Ma Minsheng , Ning Zhilong , Qiao Minghui , Awada Uchechukwu 
Published: December 2024

IntroductionPests are important factors affecting the growth of cotton, and it is a challenge to accurately detect cotton pests under complex natural conditions, such as low-light environments. This paper proposes a low-light environments cotton pest detection method, DCP-YOLOv7x, based on YOLOv7x, to address the issues of degraded image quality, difficult feature extraction, and low detection precision of cotton pests in low-light environments.MethodsThe DCP-YOLOv7x method first enhances low-quality cotton pest images using FFDNet (Fast and Flexible Denoising Convolutional Neural Network) and the EnlightenGAN low-light image enhancement network. This aims to generate high-quality pest images, reduce redundant noise, and improve target features and texture details in low-light environments. Next, the DAttention (Deformable Attention) mechanism is introduced into the SPPCSPC module of the YOLOv7x network to dynamically adjust the computation area of attention and enhance the feature extraction capability. Meanwhile, the loss function is modified, and NWD (Normalized Wasserstein Distance) is introduced to significantly improve the detection precision and convergence speed of small targets. In addition, the model detection head part is replaced with a DyHead (Dynamic Head) structure, which dynamically fuses the features at different scales by introducing dynamic convolution and multi-head attention mechanism to enhance the model's ability to cope with the problem of target morphology and location variability.ResultsThe model was fine-tuned and tested on the Exdark and Dk-CottonInsect datasets. Experimental results show that the detection Precision (P) of DCP-YOLOv7x for cotton pests is 95.9%, and the Mean Average Precision (mAP@0.5) is 95.4% under a low-light environments, showing improvements of 14.4% and 15.6%, respectively, compared to YOLOv7x. Experiments on the Exdark datasets also achieved better detection results, verifying the effectiveness of the DCP-YOLOv7x model in different low-light environments.DiscussionFast and accurate detection of cotton pests using DCP-YOLOv7x provides strong theoretical support for improving cotton quality and yield. Additionally, this method can be further integrated into agricultural edge computing devices to enhance its practical application value.
Navigating Through Innovation in Elderly’s Health: A Scoping Review of Digital Health Interventions
Authors: Hirmas-Adauy Macarena , Castillo-Laborde Carla , Awad Camila , Jasmen Anita , Mattoli Maurizio , Molina Xaviera , Olea Andrea , Matute Isabel , Soto Fernando , Rubilar Paola , Urrejola Oscar , Alfaro Tania , Abusleme Lama María Teresa , Esnouf Sophie 
Published: December 2024

ObjectivesComprehensively map and summarize digital health initiatives for the elderly and caregivers.MethodsScoping review between April and May 2022 based on Joanna Briggs methodology. Databases used included PubMed, Cochrane Library, CINAHL Plus, and Web of Science, along with grey literature and hand searches. Two reviewers independently conducted screening and eligibility phases, with a third resolving disagreements. Data were thematically analyzed.ResultsThe review included 421 documents. Most documents were published between 2013 and 2022, with a recent increase. Most studies, originating from high-income countries, focused on home applications and were mainly in the testing and validation stages. Telephones and computers were the predominant devices. Health objectives included monitoring, prevention, and treatment, with interventions utilizing directed communication and personal health monitoring for individuals, and telemedicine and decision support for healthcare providers.ConclusionIncreasing integration of technology in older adults’ lives, along with their increasing proficiency, is driving a significant rise in digital health interventions. Despite this growth, further research in middle- and low-income countries, for caregivers and evaluating effectiveness and feasibility of these technological interventions is needed.