bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of a novel alpha/beta hydrolase domain protein on tolerance of K. marxianus to lignocellulosic biomass derived inhibitors
Authors: Wu Dan , Wang Dongmei , Hong Jiong 
Published: January 2020

Abstract
Background: Lignocellulosic biomass is a promising renewable and sustainable resource for biofuels and chemicals. However, the inhibitors generated during lignocellulosic biomass pretreatment hinder microbial growth and interfere with fermentation. Studies on the tolerance to inhibitors, especially multiple inhibitors, are important in the construction of robust strains that can utilize lignocellulosic biomass. Results: The expression of an uncharted α/β hydrolase (ABHD) from Kluyveromyces marxianus was found to be up-regulated in the presence of single or multiple inhibitors; this enzyme was termed KmYME in this study. Disruption of KmYME made the yeast more susceptible to inhibitors, particularly to acetic acid, furfural and 5-hydroxymethylfurfural. KmYME was located in the mitoplast of mitochondria with targeting signals in the N-terminal 1-40 amino acid residues. Under inhibitor stress, the intracellular concentration of ATP, NAD(P) in the KmYME disrupted strain decreased. In addition, the KmYME disrupted strain lost more mitochondrial membrane potential, showed increased plasma membrane permeability, a more severe redox imbalance, and increased ROS accumulation, compared with those of the non-disrupted strain in the presence of the same inhibitors. The recombinant KmYME possessed esterase/thioesterase activity and preferred short-chain pNP aliphatic esters, long-chain acyl-CoA, and succinyl-CoA as substrates. The retro-complementation results indicated that the enzymatic activity of KmYME was necessary for the resistance to inhibitors. In addition, KmYME was also required for the resistance to other stresses including ethanol, temperature, and osmotic pressure. Disruption of two possible homologous genes in S. cerevisiae also reduced its tolerance to inhibitors. Conclusion: As an uncharted α/β hydrolase, KmYME which had esterase/thioesterase activity and located in mitochondrial matrix was identified for the first time. The esterase/thioesterase activity was necessary for the tolerance to lignocellulosic biomass derived inhibitors in K. marxianus. Disruption of KmYME reduced the tolerance to inhibitors by interfering with ATP, NAD and NADP synthesis. KmYME also correlated with tolerance to other stresses such as osmotic, ethanol, and temperature. This study may provide useful information for better understanding the resistance mechanism of multiple inhibitors, which is important in the creation of a robust strain for industrial fermentation that can use cellulosic biomass as substrate.
Characteristics of antibiotics and antibiotic resistance genes in Qingcaosha Reservoir in Yangtze River Delta, China
Authors: Xu Ting , Zhao Wanting , Guo Xueping , Hu Shuangqing , Zhang Hongchang , Huang Zhifeng , Yin Daqiang 
Published: January 2020

Abstract
Background Aquatic ecosystems are considered to be among the most important reservoirs of antibiotic resistance genes (ARGs). Drinking water sources were usually parts of lakes and rivers in Yangtze River Delta, among which Qingcaosha Reservoir is the largest river impoundment and benefit the population of more than 13 million for Shanghai city. In this study, we aimed at investigating the distribution of antibiotics and ARGs to characterize the pollution across various sites in Qingcaosha Reservoir in three seasons.Results Sulfamethoxazole, sulfamonomethoxine and penicillin G potassium salt were the dominant antibiotics and of high detection frequencies in this reservoir. Sulfonamide resistance genes ( sul1 and sul2 ) were the most prevalent and predominant genes. Higher total relative abundance of the ARGs were detected in the site closest to the inflow than those in other sites. Overall, the concentrations of antibiotics in May (spring) were relatively lower than November (autumn) and February (winter). Correlation analysis indicated sul1 , ermB and mphA had positive correlation with corresponding antibiotics in February and intI1 was also greatly positively correlated to sul1 , sul2 , ermB and mphA .Conclusion In conclusion, the antibiotics and ARGs were widespread in Qingcaosha Reservoir. Our result indicated that the drinking water reservoir might serve as gene reservoir for antibiotic resistance and mobile gene element intI1 can serve as a medium to contribute to the widespread of various ARGs. What is more, we considered that Reservoir could be served as a functional area contributing to the elimination of ARGs.
Simultaneous adsorption study of ammonium and phosphate by ZnFe-layered double hydroxide (ZnFe-LDH)-modified adsorbents.
Authors: Liu Feng , LIANG XINQIANG , Li Hua , Jin Yingbing , Zhang Chuxuan , Wang Ziwan , Tian Guangming 
Published: January 2020

Abstract
Background Trace amounts of nitrogen and phosphorus can easily trigger eutrophication damage in surface water, while cost-effective adsorption treatment is in prospect for clearing these pollutants simultaneously and efficiently. In this study, engineered adsorbents decorated with ZnFe-LDH by alkaline coprecipitation (3-6 mm, with modified ceramsite as CZF and modified volcanic rock as VZF) were prepared for NH 4 + and H 2 PO 4 - co-adsorption from aqueous solutions.Results Coexisting ions showed great effects on phosphate adsorption, especially Ca 2+ and CO 3 2- , and humic acid exhibited only a limited shielding impact on co-adsorption properties. Though comprehensive tests presented that the maximum captured amount of ammonium and phosphate occurred at pH 7, the isotherm and kinetic results suggest that two adsorbents preferentially adsorbed each pollutant. Specifically, the maximum uptake quantity of NH 4 + (15.55 mg-N g -1 ) and H 2 PO 4 - (11.21 mg-P g -1 ) were achieved by VZF and CZF, respectively, and controlled by physisorption and chemisorption distinctively, revealing that contaminants were cleared in disparate ways. Performances of seven consecutive adsorption/desorption cycles using 5% NaOH regenerant suggested that CZF retained 71.9% of PO 4 - removal efficiency, and VZF retained 47.9% of the NH 4 + adsorption rate, implying the strong reusability of ZnFe-LDH-modified adsorbents. A dynamic study has assessed that with 1 kg of combined modified adsorbents, approximately 527 L of polluted runoff flow could be continuously treated to below the National limits within one adsorption cycle.Conclusion Comparing with other parallel adsorbents, ZnFe-LDH-modified adsorbent is promising in eliminating eutrophication due to their superior capacity, stability, renewability, and non-toxicity.
Decoding the differentiation of mesenchymal stem cells into mesangial cells at the transcriptomic level
Authors: Wong Chee-Yin , Chang Yao-Ming , Tsai Yu-Shuen , Ng Wailap Victor , Cheong Soon-Keng , Chang Ting-Yu , Chung I-Fang , Lim Yang-Mooi 
Published: January 2020

Abstract
Background: Mesangial cells play an important role in the glomerulus, providing mechanical support to it, and maintaining efficient ultrafiltration of renal plasma. Loss of mesangial cells due to pathologic conditions may lead to impaired renal function. Mesenchymal stem cells (MSC) have been reported can differentiate into many cell types, including mesangial cells. However transcriptomic profiling during MSC differentiation into mesangial cells had not been studied yet. The aim of this study is to examine the pattern of transcriptomic changes during MSC differentiation into mesangial cells as well as to understand the involvement of transcription factor (TF) along the differentiation process, and finally to elucidate the relationship among TF-TF and TF-key gene or biomarkers during the differentiation of MSC into mesangial cells. Methods: Differentiation of MSC into mesangial cells was induced by co-culturing with injured mesangial cells. Total RNA from co-cultured MSC were extracted before running RNA sequencing. Generated RNA-seq data was analysed by two methods simultaneously: 1) Monotonic Feature Selector (MFSelector) to identify the key genes with ascending and descending monotonic patterns, and 2) Time-Ordered Gene Co-expression Network (TO-GCN) to construct a TF co-expression network. Results: MFSelector has identified several monotonic key genes (either ascending or descending). These identified descending monotonic key genes are related to stemness or regulation of cell cycle while ascending monotonic key genes are associated with the functions of mesangial cells. The TFs were arranged in a co-expression network in order of time by TO-GCN analysis. Additionally, not only can TO-GCN analysis classify the differentiation process into three stages: differentiation preparation, differentiation initiation and maturation, but it can also explore TF-TF-key genes regulatory relationships in the muscle contraction process. Conclusions: A systematic analysis for transcriptomic profiling of MSC differentiation into mesangial cells has been established. Key genes or biomarkers, TFs and pathways involved in differentiation of MSC-mesangial cells have been identified and the related biological implications have been discussed. Finally, we further elucidated for the first time the three main stages of mesangial cell differentiation, and the regulatory relationships between TF-TF-key genes existing in the muscle contraction process.
Theoretically designed interventions for colorectal cancer prevention: A Case of the Health Belief Model
Authors: Rakhshanderou Sakineh , Ghaffari Mohtasham , Safari-Moradabadi Ali , Maghsoudloo Maryam 
Published: January 2020

Abstract
Background According to the WHO, most chronic diseases, including cancer, can be prevented by identifying their risk factors such as unhealthy diet, smoking and physical inactivity. This study examined the effectiveness of educational interventions on colorectal cancer prevention-nutritional behaviors by employing a health belief model among the personnel of Shahid Beheshti University of Medical Sciences.Methods In this experimental study, 110 employees of Shahid Beheshti University of Medical Sciences were randomly divided into two groups (intervention and control) with cluster sampling. The data gathering tool was a researcher-made questionnaire containing two parts of 10-dimensional information and health belief model constructs. Educational intervention was conducted for one month and in four sessions in the form of classroom lecture, pamphlet, educational text messages via mobile phones and educational pamphlets through the office automation system. Two groups were evaluated in two stages, pre-test and post-test. Data were analyzed using SPSS-18 software, paired t-test and independent t-test (intergroup comparisons).Results Two groups were evaluated for variables such as age, sex, education level and family history of colorectal cancer, and there was no significant differences between the two groups (P <0.05). After the two months since intervention, except for the mean score of perceived barriers, which was not significant after intervention, the mean scores of knowledge, perceived susceptibility, perceived severity, perceived benefits, perceived self-efficacy, behavioral intention, and preventive behaviors were significantly increased after the intervention in the intervention group compared to the control group (P> 0.05).Conclusion Implementation of educational intervention based on health belief model was effective for the personnel, and can enhance the preventative nutritional behaviors related to colorectal cancer.
Longitudinal observation of OCT imaging is a valuable tool to monitor primary vitreoretinal lymphoma treated with intravitreal injections of methotrexate
Authors: Zhao Huiying , Wang Xiaona , Mao Yu , Peng Xiaoyan 
Published: January 2020

Abstract
Background: Developing objective and repeatable indicators to evaluate the efficacy of PVRL treatment is important. The quantification of vitreous cells is a traditional criterion; however slight changes are difficult to ascertain. Spectral domain optical coherence tomography (SD-OCT) is objective, repeatable, and easily explained. The purpose of this study is to provide a longitudinal observation of OCT in PVRL treated with intravitreal injections of methotrexate (MTX) and to evaluate the utility of OCT in monitoring responsiveness of PVRL to treatment. Methods: The medical records of patients with biopsy-positive PVRL attending our hospital between January 2016 and September 2017 who received intravitreal injections of MTX were included in this study. Pre- and post-treatment OCT images were reviewed independently by two researchers. Results: Of the 24 cases reviewed, 10 patients (18 eyes) were included. SD-OCT abnormalities at the initial visit included vitreous cells (18/18), OR (outer retina) fuzzy borders (12/18), PED (pigment epithelium detachments) (9/18), subretinal hyperreflective infiltration (3/18), intraretinal infiltration (8/18), and SRF (subretinal fluid) (4/18). Post induction treatment, SRF in cases with RD (retinal detachment) was absorbed, and subretinal fibrosis appeared. Other lesions were significantly reduced. Post consolidation treatment, OR fuzzy borders, PED and SRF disappeared in 2 eyes, intraretinal infiltration disappeared in 1 eye, and other abnormalities further improved. Additionally, retinal fibrosis was observed in 3 eyes. One month post maintenance treatment, all abnormalities observed at the first visit vanished. At the last visit, OCT showed subretinal fibrosis and in 3 eyes (16.7%), the disruption of outer retina in 9 eyes (50%) and thinning of the whole layer in 4 eyes (22.2%). Conclusions: Our observations reveal that characteristic OCT features in PVRL patients can reduce gradually and finally vanish with therapy. We propose that SD-OCT may be employed to monitor the responsiveness of PVRL to treatment, which may influence decision making in the management of this disease.
Design, Synthesis and Cytotoxic Activity of Spiro(oxindole-3-3'- pyrrolidine) Derivatives
Authors: Konyar Dilan , Andac Cenk A. , Buyukbingol Erdem 
Published: January 2020

Background:
Spiro[pyrrolidine-3,3'-oxindole] compounds are reported to be highly
bioactive natural and synthetic products. Initially, spirooxindole alkaloids were isolated from plants
of the Apocynaceae and Rubiaceae families, which were found to have a common scaffold,
spiro[pyrrolidine-3,3'-oxindole], exhibiting anticancer activities., we specifically aimed at the synthesis,
characterization and anticancer activity of novel spiro[pyrrolidine-3,3' -oxindole] derivatives,
compounds 6a-c and 7.

Methods:
The synthesis was initiated by Knovenegal condensation of indole-2-one with an appropriate
benzaldehyde in presence of piperidine to afford compounds 3a-c. Compounds 6a-c were
synthesized by an asymmetric 1,3-dipolar cycloaddition between compounds 3a-c and (2S, 3R)-2,
3, 5, 6-tetrahydro-2, 3-diphenyl-1, 4-oxazin-6-one, which is an intermediate compound formed by
the Schiff base reaction between 3-methyl-butanal and (2S, 3R)-2, 3, 5, 6-tetrahydro-2, 3-diphenyl-
1,4-oxazin-6-one, in presence of molecular sieves (4Å) under argon atmosphere. Compound 6a was
then reacted with ethylamine-HCl in THF at room temperature to yield compound 7.

Results:
Cytotoxic effects of the compounds synthesized were determined on Huh7, MV, HCT116
and MCF7 cancer cell lines by the NCI-60 Sulforhodamine B Assay, using (S)-(+)-Camptothecin as
a positive control. In general, target compounds showed better cytotoxic activities against the
MCF7 and HCT116 cancer cell lines. It was found that compound 7 exhibited the most potent inhibitory
activity with IC50 values of 4.8 µM, 3.9 µM, 14.9 µM and 8.2 µM against the MCF7,
HCT116, MV and Huh7 cell lines, respectively.

Conclusion:
It was determined that compounds 6a&6b possess C6'(S)|C8'(R)|C9'(R) stereochemistry
and compound 7 adopts C2'(S)|C4'(R)|C5'(R) stereochemistry. Cytotoxicity studies suggest that
compound 7 gave rise to the highest anticancer activity against MCF7, HCT116, and Huh7 cancer
cell lines.
PAKAIAN PANGULU DI NAGARI GUNUANG KOTA PADANGPANJANG PROVINSI SUMATERA BARAT
Authors: Anwar Robi , Sastra Andar Indra , Zebua Edward 
Published: January 2020

AbstrakPenelitian ini mendiskripsikan tentang bentuk pakaian pangulu. Fokus penelitian ini adalah pakaian pangulu di Nagari Gunuang Kota Padangpanjang provinsi Sumatera Barat. Pakaian pangulu merupakan pakaian kebesaran seorang pemuka adat suatu kaum yang menyandang gelar pangulu. Pakaian pangulu mencerminkan nilai kewibawaan seorang pemimpin yang terlihat secara visual. Metode dalam penelitian ini menggunakan pendekatan kualitatif dengan tipe deskriptif. Teknik pengumpulan data dilakukan dengan studi pustaka, observasi, dan dokumentasi. Penelitian ini membahas tentang bentuk dan makna pakaian pangulu. Hasil tentang pakaian pangulu berupa pakaian adat untuk seorang pemimpin adat di Nagari Gunuang Kota Padangpanjang. Pakaian itu terdiri dari destar/saluak, baju hitam longgar/baju gadang, kain sandang/salempang, celana/sarawa, sarung/sisampiang, ikat pinggang/cawek, keris/karih, tongkat/tungkek. Makna dari pakaian pangulu adalah wibawa seorang pemimpin kaum dalam mengayomi anak kemenakan dan mampu menjadi hakim pada masyarakat.Kata Kunci: pakaian, pangulu, bentuk, makna.AbstractThis study describes the form of pangulu clothing. The focus of this research is pangulu clothing in Nagari Gunuang, Padangpanjang, West Sumatra province. Pangulu clothing is an oversized clothing of an adat leader, a people bearing the title pangulu. Pangulu's clothes reflect the dignity of a leader visually. The method in this study uses a qualitative approach with descriptive type. The technique of data collection is done by literature study, observation, and documentation. This study discusses the shape and meaning of pangulu clothing. The results of the pangulu clothing in the form of traditional clothing for a traditional leader in Nagari Gunuang, Padangpanjang City. The clothes consist of a sash/saluak, loose black clothes/gadang clothes, clothing/salempang cloth, pants/sarawa, sarongs/sisampiang, belts / cawek, keris/karih, sticks/tungkek. The meaning of pangulu's clothing is the authority of a leader of the people in protecting the children of nephews and being able to be judges in the community.. Keywords: clothes, pangulu, shape, meaning.
KESESUAIAN POLA CELANA SISTEM CHARMANT PADA WANITA DEWASA INDONESIA BERTUBUH IDEAL
Authors: Hanifah Hanifah , Ernawati Ernawati 
Published: January 2020

AbstrakTujuan penelitian untuk mendeskripsikan kelemahan, cara memperbaiki, serta kesesuaian pola celana wanita sistem Charmant terhadap wanita bertubuh ideal Indonesia. Metode penelitian yang digunakan adalah penelitian terapan. Objek penelitian yaitu pola celana wanita sistem Charmant yang diuji cobakan pada wanita bertubuh ideal Indonesia, dengan tinggi 165 cm, berat 57 kg. Dinilai oleh 5 orang panelis yang ahli dibidang pola busana wanita. Instrumen penelitian yang digunakan adalah kuesioner/angket memakai skala likerts. Teknik analisa data menggunakan statistik deskriptif berupa perhitungan rumus persentase menggunakan microsoft exel. Hasil penelitian menunjukkan pola celana wanita sistem Charmant terhadap wanita bertubuh ideal Indonesia, mempunyai 2 kelemahan berdasarkan penilaian dari 5 orang panelis diantaranya yaitu: 1) lingkar duduk sempit 4 cm, 2) tinggi duduk panjang 3 cm. Cara memperbaikinya yaitu: 1) lingkar duduk ditambahkan 1 cm pada pola depan dan belakang, 2) tinggi duduk dikurangi sebanyak 3 cm.Kata Kunci: kesesuaian, celana, charmant, wanita ideal.AbstractThe purpose of this study is to describe the weaknesses, how to improve, and the suitability of Charment’swomen's skrit pattern towards ideal Indonesian female posture. The method uses applied study. The data analysis was performed using descriptive statistics in the form of calculating formula percentages using Microsoft Excel. The object of this study was the Charmant’s women skirt pattern which were tested on an ideal Indonesian female posture, with a height of 165 cm, weight of 57 kg. Judged by 5 panelists who are experts in women's clothing. The study instrument was a questionnaire / questionnaire using the Likerts scale. The results showed that the Charmant’swomen skirt pattern towards an ideal Indonesian female posture had 5 weaknesses based on the assessment from 5 panelists which were : 1) circumference narrow sitting of 3 cm, 2) sitting high over 3 cm. How to improve it are: 1 cireumference narrow sitting add by 1 cm on the front and back side, 2) sitting high shortened by 3 cm. Keyword: suitability, skirt, charmant, ideal woman posture.
PENGARUH MORDAN TAWAS DAN CUKA TERHADAP HASIL PEWARNAAN ECO PRINT BAHAN KATUN MENGGUNAKAN DAUN JATI (Tectona Grandis)
Authors: Masyitoh Fitri , Ernawati Ernawati 
Published: January 2020

AbstrakPenelitian ini dilatar belakangi sebagai upaya pemanfaatan pewarna alam daun jati untuk membuat motif tekstil dengan teknik pewarnaan eco print yang ramah lingkungan. Penelitian ini bertujuan untuk mendeskripsikan nama warna (Hue), gelap terang (Value) dan kejelasan bentuk yang dihasilkan dari pewarnaan eco print bahan katun menggunakan daun jati (Tectona grandis) dengan mordan tawas dan cuka. Jenis penelitian ini merupakan penelitian eksperimen. Jenis data berupa data primer.pewarnaan eco print dengan mordan tawas menghasilkan nama warna Pomdadour Purple, warna kurang terang dan kejelasan bentuk termasuk kategori sangat jelas. Pada pewarnaan eco print dengan mordan cuka menghasilkan nama warna Muddy Waters Brown, warna terang dan kejelasan bentuk termasuk kategori cukup jelas. Kata Kunci: eco print, daun jati, mordan.AbstractThis research is motivated as an effort to use natural teak leaf dyes to make textile motifs with eco-friendly coloring techniques. This study aims to describe the color name (Hue), dark darkness (Value) and the clarity of the shape resulting from the staining of eco print cotton using teak leaves (Tectona grandis) with mordan alum and vinegar. This type of research is experimental research. The type of data in the form of primary data. Eco print staining with mordan alum produces the color name Pomdadour Purple, the color is not bright and the clarity of the shape, including the category is very clear. On eco print staining with mordan vinegar produces Muddy Waters Brown color name, bright color and shape clarity including the category is quite clear.. Keywords: ecoprint, teak leaves, mordant.