bluuebunny/crossref_metadata_2025_split huggingface dataset:
RANCANG BANGUNG MODEL PEMBELAJARAN FLIPPED CLASSROOM SEBAGAI SOLUSI PENINGKATAN DAYA BELAJAR MANDIRI MAHASISWA
Authors: Sukri Hanifudin , Fatah Doni Abdul 
Published: December 2020

Pesatnya perkembangan teknologi informasi memudahkan proses belajar mengajar antara dosen dan mahasiswa. Metode belajar mahasiswa pun ikut bergeser dari penggunaan media belajar text book pada media gadget yang merupakan “dunia” generasi millenial yang menawarkan lebih banyak model referensi seperti video, simulasi, dan sebagainya. Model pembelajaran konvensional yang bertumpu pada dosen atau Teacher Center Learning(TCL) digantikan dengan Student Centered Learning (SCL). Penerapan strategi dan metode pembelajaran yang tepat sesuai dengan perkembangan jaman dan teknologi akan meningkatkan efektifitas pembelajaran mahasiswa dan dapat meningkatkan daya belajar mandiri mahasiswa, dengan metode Flipped Classroom model pembelajaran tradisional dibalik, yang biasanya materi diberikan di kelas dan mahasiswa mengerjakan tugas di rumah. Konsep Flipped Classroom mencakup active learning, keterlibatan mahasiswa, dan podcasting, materi terlebih dahulu diberikan melalui video pembelajaran yang harus ditonton mahasiswa di rumah. Sebaliknya, sesi belajar di kelas digunakan untuk diskusi kelompok, diskusi dengan dosen tentang materi yang sulit untuk dipahami pada saat belajar mandiri dan mengerjakan tugas. Setelah dilakukan evaluasi hasil pembelajaran Flipped Classroom didapatkan hasil sebanyak 69,2% media sosial menjadi bagian penting dalam pembelajaran, 76,9% mahasiswa lebih menyukai pembelajaran dalam bentuk video dibanding dengan model tradisional, 84,6% mengiyakan model pembelajaran ini dapat meningkatkan keinginan belajar mandiri mahasiswa, 89,2% belajar dengan menggunakan metode ini memberi manfaat untuk pendidikan masa depan, 69,2% model pembelajaran ini memberikan banyak waktu mahasiswa untuk melakukan kegiatan di laboratorium, 57,1% mahasiswa dengan menggunakan metode ini memberikan kesempatan lebih besar komunikasi atau interaksi dengan mahasiswa yang lainnya dan sebesar 64,3 % mahasiswa ternyata lebih termotivasi belajar menggunakan metode Flipped Classroom
Drug Induced Hybrid Electrospun PLA:Cell Derived Extracellular Matrix Scaffolds Support the Survival and Function of Human Primary Hepatocytes
Authors: Grant Rhiannon , Hallett John , Forbes Stuart , Hay David , Callanan Anthony 
Published: November 2020

Abstract
An exponential increase in liver disease is driving a critical shortage of donor livers for patient transplant. In the UK alone, 58 people died in 2019 while waiting for a donor organ. A solution is sought in the form of tissue-engineered devices which support the survival and function of primary human hepatocytes. Previous work has shown that biofunctionalization of electrospun scaffolds influences hepatocytes. This study assesses the impact of drug-derived ECM on primary human hepatocytes (PHHs); a gold standard research resource. Hepatocytes seeded onto electrospun PLA scaffolds were subjected to drug treatment using histone deacetylase inhibitors. These cells were stripped from the scaffolds to leave behind their ECM. The resulting ECM-PLA scaffolds were seeded with PHHs and cultured for 24/72/120 hours. Scanning electron microscopy (SEM), mechanical and biochemical quantification, histology, and gene expression analyses were performed on the scaffolds. Results demonstrate PHHs are significantly influenced by the drug derived ECM:PLA scaffolds, with alterations in albumin production and gene expression demonstrated. Creating multidimensional scaffolds like these provides a method of creating tailored environments for liver bioengineering and the investigation of cell matrix interactions and is a step on the path to providing lab grown organoids for patient transplant.
Arsenic induced patterns of auto-grooming response over time in Macrobrachium lamarrei: a study on behavioural plasticity
Authors: Munshi Chayan , Das Deepyaman , Biswas Partho , Sen Kosturi , Mondal Nisha , Mukherjee Shreya , Bhowmick Rajasree , Kundu Piyali 
Published: December 2020

Abstract
Behavioural toxicity is a very applicable area in the vast field of ecotoxicology where,assessment of the alteration of behaviour is an effective tool to evaluate neural activity in an organism. In our present study, we have shown that arsenic trioxide exposure can stimulate repetitive grooming activity in a freshwater prawn, Macrobrachium lamarrei; however, over time the organism can modulate the grooming performances to less. We predict that repetition in the grooming behaviour is a case of neurotoxicity by arsenic and induction of Autism Spectrum Disorder (ASD) within a short period of exposure time.
Using a Mixed Method Approach to Tailor the Implementation of a Participatory Total Worker Health® Program in Public Healthcare Facilities
Authors: Nobrega Suzanne , Morocho Cesar , Robertson Michelle M. , Kurowski Alicia , Rice Serena , Punnett Laura 
Published: December 2020

Abstract
Background: The Total Worker Health program represents a holistic approach to advancing worker well-being that combines occupational safety and health practices with other workplace policies and programs that are not traditionally linked. Total Worker Health requires new types of interdisciplinary collaboration and programmatic coordination. Pre-implementation assessment is thus important to plan for successful organizational “fit” when the program is introduced. This study prospectively identified potential implementation facilitators and barriers among five public healthcare facilities that had already agreed to participate in a study to implement and evaluate the Healthy Workplace Participatory Program.Methods: A mixed methods baseline assessment comprised an online survey and follow-up interviews. Key organizational and labor leaders were asked to identify resources and skills available for successful program implementation; potential barriers inside or outside the organization; and key performance indicators. Findings were presented to implementation stakeholders in study sites and used for planning how to tailor implementation to fit the organizational context and to provide the resources needed for success. Results: Potential facilitators included leaders’ willingness and commitment to develop interventions addressing a broad range of occupational safety and well-being health priorities (consistent with Total Worker Health); existing staff expertise in occupational safety and health; favorable attitudes regarding expected program outcomes; and positive alignment between the program and organizational mission and values. Potential implementation barriers included limited staff time to attend meetings, limited resources to support locally designed interventions, and poor management communication systems. Examples of tailoring strategies included extending time and effort to recruit leaders and workers while gaining their trust and securing program resources; developing sample program communication templates to strength health and safety communication efforts; and providing detailed training around issue selection procedures, communication, local intervention development, and change management. Conclusions: The prospective identification of potential facilitators and barriers represents a useful strategy for tailoring the implementation of a participatory, Total Worker Health program. Trial Registration: ClinicalTrials.gov NCT 04251429. Registered January 31, 2020 – Retrospectively registered. https://clinicaltrials.gov/ct2/show/record/NCT04251429.
Upgrading of Food Waste in-vessel Composting Process: Effects of Biochar-rich Digestate Addition
Authors: Chaher Nour El houda , Nassour Abdallah , Nelles Michael , Hamdi Moktar 
Published: December 2020

Abstract
Nowadays, Tunisia faces challenging environmental and energic issues which concern mainly the implementation of an appropriate solid waste management system dealing with the high production of biowaste in one hand and the increased need for water and energy resources in the other hand. Therefore, the current study intended to develop a technical concept on closed cycle ‘biowaste to bioenergy’, treating mainly food waste (FW) through combined biological processes. In this approach, FW anaerobic digestion (AD) residue was destined to feed FW in vessel-composting as a valuable input material. To this end, the produced digestates from amended and unamended anaerobic reactors were entirely examined to select later the most appropriate AD-effluent. Therefore, the identification of the convenient digestate was achieved based on technical criteria ; moisture content (MC), C : N ratio and heavy metals concentrations. The findings showed that the digestate obtained from different digesters was characterized by a high moisture content which inspired its utilization as an unconventional moisturizing agent (MA) reducing the fresh water consumption during the composting process. Because of the high C : N ratio, relatively significant rate of the needed macro and micro-nutrients, the exploitation of biochar-rich digestate was recomended to be used as an unconventional MA in one hand and an aerobic co-substrate booster in the other hand. Results showed that D3 addition promoted the composting process performance comparing to the blank test (A1). Indeed, the thermophilic phase of the amended reactor (A2) lasted 15 days and reached higher temperatures of about 67 °C, while the unamended one (A1) was characterized by a thermophilic temperature of around 63 °C indicating that the end-products were of a pathogen-free compost. When it comes to the physico-chemical factors examined demonstrating that the biological conditions were sufficiently developed. The findings showed overall decreasing profiles during the composting period for moisture, C : N ratio as well as nitrification index (NI). From the quality-point of view, it was found that heavy metal concentrations had lower limits than those values set by German standards. Moreover, all the compost samples appeared to be stable and classified as class IV and V end-product.
Comparative Transcriptomics Provides Insight into Rind Color Polymorphism in Watermelon
Authors: Liu Dongming , Yang Huihui , Sun Dongling , Zhang Minjuan , Dou Junling , Yang Sen , Zhu Huayu , Hu Jianbin , Sun Shouru , Yang Luming 
Published: December 2020

Abstract
Background: As an important agronomic trait affecting breeding and consumption choice, the rind color of watermelon [Citrullus lanatus (Thunb.) Matsum. and Nakai] receives considerable attention from breeders. Although rind color occupies an important place in watermelon breeding work, the underlying molecular mechanism governing this complex trait has still not been determined. Results: In the present study, chlorophyll and carotenoid contents were hypothesized to be the main factors determining rind color. Clearly different structures and numbers of chloroplasts and grana lamellae were found among materials with different rind colors. To study the genes involved in the coloration process of the yellow, dark green, light green, and light green-gray rinds, 84,516 unigenes were obtained from the rind transcriptome. Through DEGs screening and pathway analysis of chlorophyll and carotenoid mechanism, the genes with abnormal expression levels were identified. Then, the nucleotide sequences and expression levels of the putative genes responsible for different rind colors were studied.Conclusions: This study provides a comprehensive insight on the factors affecting rind coloration and investigated expression levels of the critical genes concerning pigment metabolism in watermelon. These results would help to analyze the molecular mechanisms of rind coloration regulation and development of the related organelles.
Comparative metabolites profiling of Polygonatum cyrtonema Hua seed during sand storage and germination
Authors: Jin Qing , Tong Jinfeng , Zhang Wenwu , Xia Long , Zhu Xiaoyun , Ran Rui , Chang Yan , Zhang Jie , Cai Yongping 
Published: December 2020

Abstract
Background: The seeds of Polygonatum cyrtonema Hua have dormancy phenomenon. Previous studies have shown that sand storage factors effects of the seed dormancy of P. cyrtonema Hua seeds and enhance the seed germination process. Subsequently, metabolic activities and different changes during the sand storage and germination process of P. cyrtonema Hua seed has not been heavily researched.Results: In this study the changes in the metabolites of P. cyrtonema Hua seeds at different sand storage times and germination stages, we used untargeted metabolomics to determine them. Most of the sugar and glycoside contents in seed coat increased after 30 d on the other hand, in peeled seeds increased at 30 d and decreased at 60 d after sand storage treatment. The content of proline and benzoic acid decreased in the seed coat after sand storage. PCA, OPLS-DA and HCA showed that the contents of most metabolites increased after 7 d and decreased after 14 d of seed germination. The process of 7 d to 14 d was the key stage of seed germination of P. cyrtonema Hua. Differential metabolic pathway analysis showed that seed germination was controlled by multiple metabolic pathways. Metabolic correlation revealed the interdependence between seed germination metabolites and metabolic pathways. Conclusion: Sand storage can significantly increase the rate of seed germination and play a vital role in seed dormancy of P. cyrtonema Hua. There was inherent differences in metabolites during different storage time and germination stages in P. cyrtonema Hua. Our work provides a first glimpse of the metabolome in seed germination of P. cyrtonema Hua, and provides a valuable informations for revealing the mechanism of breaking seed dormancy.
Genetic analysis of DNA methylation in dyslipidemia: a case-control study
Authors: Ma Yi-Tong , Liu Shuai , Li Yang , Wei Xian , Adi Dilare , Wang Yong-Tao , Han Min , Liu Fen , Chen Bang-Dang , Li Xiao-Mei , Yang Yi-Ning , Fu Zhen-Yan 
Published: December 2020

Abstract
In our previous study, we explored the relationship between TBL2 gene DNA methylation and high-low-density lipoproteinemia (Hyper-LDL). However, Hyper-LDL is only one type of dyslipidemia. In order to expand the scope of clinical application, we explored the correlation between DNA methylation of genes related to lipid metabolism and dyslipidemia in this study. This study is a case-control study. A total of 180 samples were included in this study from the Heart Center of the First Affiliated Hospital of Xinjiang Medical University. The BSAS method was used to detect the DNA methylation levels and haplotypes of AMFR, FBXW7, INSIG1, INSIG2, MBTPS1 and GRINA genes. A total of 259 CpG sites and 14 regions were detected. The study found that a total of 24 CpG sites DNA methylation and 20 haplotypes were statistically different. The GRINA gene DNA methylation level in the dyslipidemia group was higher than that in the control group (2.68 vs 2.36, p = 0.04). ttttttttttttcttttttttttt is significant methylation haplotype of GRINA (p=0.017). Through logistics analysis, it is found that GRINA gene DNA methylation is an independent risk factor for dyslipidemia, and the increase of GRINA gene DNA methylation level will increase the prevalence of dyslipidemia.
EARN: an ensemble machine learning algorithm to predict driver genes in metastatic breast cancer
Authors: Mirsadeghi Leila , Hosseini Reza Haji , Banaei-Moghaddam Ali Mohammad , Kavousi Kaveh 
Published: December 2020

Abstract
BackgroundToday, there are a lot of markers on the prognosis and diagnosis of complex diseases such as primary breast cancer. However, our understanding of the drivers that influence cancer aggression is limited.MethodsIn this work, we study somatic mutation data consists of 450 metastatic breast tumor samples from cBio Cancer Genomics Portal. We use four software tools to extract features from this data. Then, an ensemble classifier (EC) learning algorithm called EARN (Ensemble of Artificial Neural Network, Random Forest, and non-linear Support Vector Machine) is proposed to evaluate plausible driver genes for metastatic breast cancer (MBCA). ResultsThis study is an attempt to focus on the findings in several aspects of MBCA prognosis and diagnosis. First, drivers and passengers predicted by SVM, ANN, RF, and EARN are introduced. Second, biological inferences of predictions based on gene set enrichment analysis are discussed. Third, statistical validation and comparison of all learning methods based on evaluation metrics are done. Finally, the pathway enrichment analysis (PEA) using ReactomeFIVIz tool (FDR<0.03) for the top 100 genes predicted by EARN leads us to propose a new gene set panel for MBCA, including HDAC3, ABAT, GRIN1, PLCB1, and KPNA2 as well as NCOR1, TBL1XR1, SIRT4, KRAS, CACNA1E, PRKCG, GPS2, SIN3A, ACTB, KDM6B, and PRMT1. Furthermore, we compare results for MBCA to other outputs regarding 983 primary tumor samples of breast invasive carcinoma (BRCA) obtained from the Cancer Genome Atlas (TCGA). The comparison between outputs shows that ROC-AUC reached 99.24% using EARN for MBCA and 99.79% for BRCA. This statistical result is better than three individual classifiers in each case.ConclusionsThis research using an integrative approach assists precision oncologists to design compact targeted panels that eliminate the need for whole-genome/exome sequencing.
Health Determinants of Life Satisfaction Among Elderly Persons: A multivariate Analysis
Authors: Hong Rou-Qin , Rahman Hanif Abdul , Ali Mursidi , Hoon Chang-Yau , Slesman Ly , Tengah Armah , Yusof Yusnani Mohd , Abdul-Mumin Khadizah H. 
Published: December 2020

Abstract
Purpose: Level of satisfaction is significantly influenced by health-related factors across human life course. Multidimensions of health and other factors such as religion and culture influence life satisfaction that differ across different societies. Prior research shows that there are variations in the relationship between health factors and life-satisfaction between Western and Asian countries. Nevertheless, it is still unknown to what extend health factors are related to life satisfaction in Brunei Darussalam—a country with small population but increasing in proportion of elderly population. This study aimed to explore health determinants of life satisfaction among elderly population in Brunei Darussalam. Methods: A cross-sectional study on a sample of 645 adults aged above 50 years, recruited nationally. Descriptive statistics and multivariate regression analysis were applied. Results: A robust model (R2=49.3%, alpha>0.7) suggested that life satisfaction in this population could largely be explained by musculoskeletal pain, difficulty to perform daily activities, psychological and emotional issues, and family support. Gender has significant moderating effects on this relationship. Conclusion: Impacts of life satisfaction from health, social, cultural, and related perspectives were discussed. These findings in this research may contribute to ongoing multi-sectorial efforts to design a comprehensive model of elderly care to achieve high quality of life.
Who are the beneficiaries and what are the reasons for non-utilization of respite care? A cross-sectional study on family caregivers
Authors: Huang Jianan , Münzel Nadja , Scheel-Sailer Anke , Gemperli Armin 
Published: December 2020

Abstract
BackgroundFamily caregivers assume substantial caregiving responsibilities for persons with chronic conditions, which leads to negative impact on their lives. Respite care is provided as a temporary relief for them. The design of appropriate respite care programs requires the identification of beneficiary subgroups for different types of services. The objectives of the study were to quantify the uptake of respite care services by family caregivers of persons with spinal cord injury, and to identify the main beneficiaries of the respective offers and the reasons for non-use.MethodsA cross-sectional survey of family caregivers of persons with spinal cord injury was conducted nationwide in Switzerland. The use of 11 different respite care services during the last 12 months was investigated, along with caregivers’ reasons for not using any respite service. Classification trees were used to characterize the beneficiaries and reasons for not using respite services.ResultsOne-third of family caregivers used at least one type of respite care service during the last 12 months. Utilization of respite care was found to be greater among those who employed professional home care (57% vs 24% among those without professional home care). There were marked cantonal differences in the utilization of respite care. The primary reason for not using respite services was “no demand” (80% among non-users of respite services), mainly among caregivers who were less emotionally affected by their caregiving tasks. Conclusions Utilization of respite care services primarily depends on living arrangements and place of residency and less on the functional status of the care recipient. Programs should thus be tailored to the cultural context of their potential users. This is best achieved through coordination with local health care professionals who can identify the needs, provide information, initiate referrals, and integrate the care into a larger support plan.
Construction and Characterization of EGFP Reporter Plasmid Harboring Putative Human RAX Promoter for in vitro Monitoring of Retinal Progenitor Cells Identity
Authors: Atefi Atefeh , kojouri Pendar Shojaei , Karamali Fereshteh , Irani Shiva , Esfahani Mohammad Hossein Nasr 
Published: December 2020

Abstract
Background : In retinal degenerative disease, progressive and debilitating conditions result in deterioration of retinal cells and visual loss. In human, retina lacks the inherent capacity for regeneration. Therefore, regeneration of retinal layer from human retinal progenitor cells (hRPCs) is a challenging task and restricted in vitro maintenance of hRPCs remains as the main hurdle. Retina and anterior neural fold homeobox gene ( RAX ) play critical roles in developing retina and maintenance of hRPCs. In this study, for the first time regulatory regions of human RAX gene with potential promoter activity were experimentally investigated. Results: For this purpose, after in silico analysis of regulatory regions of human RAX gene, the expression of EGFP reporter derived by putative promoter sequences was first evaluated in 293T cells and then in hRPCS derived from human embryonic stem cells. The candidate region ( RAX -3258bp) showed the highest EGFP expression in hRPCs. This reporter construct can be used for invitro monitoring of hRPC identity and verification of an efficient culture medium for maintenance of these cells. Conclusions: Furthermore, our findings provide a platform for better insight into regulatory regions of human RAX gene and molecular mechanisms underlying its vital functions in retina development.