bluuebunny/crossref_metadata_2025_split huggingface dataset:
Targeting AGTR1/NF-κB /CXCR4 axis by miR-155 attenuates oncogenesis in Glioblastoma
Authors: Singh Anukriti , Srivastava Nidhi , Ateeq Bushra 
Published: October 2019

AbstractGlioblastoma (GBM) represents the most aggressive malignancy of the brain. Angiotensin II Receptor Type 1 (AGTR1) upregulation has been associated with proliferative and infiltrative properties of glioma cells. However, the underlying mechanism of AGTR1 upregulation in GBM is still unexplored. To understand the post-transcriptional regulation ofAGTR1in GBM, we screened 3’untranslated region (3’UTR) ofAGTR1by using prediction algorithms for binding of miRNA. Interestingly, miR-155 showed conserved binding on the 3’UTR ofAGTR1, subsequently confirmed byAGTR1-3’UTR-luciferase reporter assay. Furthermore, stable miR-155 overexpressing GBM cells show decrease in AGTR1-mediated cell proliferation, invasion, foci formation and anchorage-independent growth. Strikingly, immunodeficient mice implanted with stable miR-155 overexpressing SNB19 cells show remarkable reduction (∼95%) in tumor burden compared to control. Notably, miR-155 attenuates NF-κB signaling downstream of AGTR1 leading to reduced CXCR4 and AGTR1 levels. Mechanistically, miR-155 mitigates AGTR1-mediated, angiogenesis, epithelial-to-mesenchymal transition, stemness, ERK/MAPK signaling and promotes apoptosis. Similar effects in cell-based assays were observed by using pharmacological inhibitor of IκB Kinase (IKK) complex. Taken together, we established that miRNA-155 post-transcriptionally regulatesAGTR1expression, abrogates AGTR1/NF-κB/CXCR4 signaling axis and elicits pleiotropic anticancer effects. This study opens new avenues for using IKK inhibitors and miRNA-155 replacement therapies for the treatment of AGTR1-positive malignancies.
GAD: a Python script for dividing genome annotation files into feature-based files
Authors: Karam Ahmed , Yasser Norhan 
Published: October 2019

AbstractNowadays, manipulating and analyzing publicly available genomic datasets become a daily task in bioinformatics and genomics laboratories. The release of several genome sequencing projects prompts bioinformaticians to develop automated scripts and pipelines which analyze genomic datasets in particular gene annotation pipelines. Handling genome annotation files with fully-featured programs used by non-developers is necessary, furthermore, accelerating genomic data analysis with a focus on diminishing the genome annotation and sequence files based on specific features is required. Consequently, to extract genome features from GTF or GFF3 in a precise manner, GAD script (https://github.com/bio-projects/GAD) provides a simple graphical user interface which interpreted by all python versions installed in different operating systems. GAD script contains unique entry widgets which are capable to analyze multiple genome sequence and annotation files by a click. With highly influential coded functions, genome features such upstream genes, downstream genes, intergenic regions, genes, transcripts, exons, introns, coding sequences, five prime untranslated regions, and three prime untranslated regions and other ambiguous sequence ontology terms will be extracted. GAD script outputs the results in diverse file formats such as BED, GTF/GFF3 and FASTA files which supported by other bioinformatics programs. Our script could be incorporated into various pipelines in all genomics laboratories with the aim of accelerating data analysis.
CTRL: a label-free method for dynamic measurement of single-cell volume
Authors: Yao Kai , Rochman Nash D. , Sun Sean X. 
Published: October 2019

AbstractMeasuring the physical size of the cell is valuable in understanding cell growth control. Current single-cell volume measurement methods for mammalian cells are labor-intensive, inflexible, and can cause cell damage. We introduce CTRL: Cell Topography Reconstruction Learner, a label-free technique incorporating Deep Learning and Fluorescence Exclusion for reconstructing cell topography and estimating mammalian cell volume from DIC microscopy images alone. The method achieves quantitative accuracy, requires minimal sample preparation, and applies to extensive biological and experimental conditions. Using this method, we observe a noticeable reduction in cell size fluctuations during cell cycle, which is consistent with the presence of a cell size checkpoint. (https://GitHub.com/sxslabjhu/CTRL)
Distributed dissipative filtering for flexible manipulator with randomly occurring uncertainties and missing data
Authors: Zhang Xiaodong , Xiao Tao 
Published: October 2019

Purpose
The purpose of this paper is to investigate the dissipative filtering problem for a flexible manipulator (FM) with randomly occurring uncertainties and randomly occurring missing data.

Design/methodology/approach
The randomly occurring phenomena during the filtering procedure are described by Bernoulli sequences. Based on the idea of dissipative theory, the distributed filtering error augmented system is derived for ensuring the prescribed dissipative performance.

Findings
By constructing appropriate Lyapunov function, sufficient dissipative filtering conditions are derived such that the filtering error can be approaching zero. Then, the desired distributed filter gains are designed with the help of matrix transformation.

Originality/value
The merit of this paper is proposing a novel distributed filtering framework for an FM with external disturbance under the dissipative framework, which can provide a more applicable filter design.
Exploring the dual effects of perceived waiting on healthcare service satisfaction among outpatients
Authors: Liu Yi-Fen , Liao Jun-Fang , Jou Jacob 
Published: October 2019

PurposeThe purpose of this paper is to explore healthcare waiting time and the negative and positive effects (i.e. the dual effects) it has on outpatient satisfaction.Design/methodology/approachSelf-administered surveys with 334 outpatients and follow-up interviews with 20 outpatients in three large hospitals in Taiwan were conducted to collect data.FindingsQuantitative surveys demonstrated that perceived waiting time correlated with satisfaction negatively first but then positively. Satisfaction also correlated with doctor reputation and patient sociability. Follow-up qualitative interviews further revealed that, for some patients, waiting contributed positively to patient evaluations through signaling better healthcare quality and facilitating social interaction.Originality/valueThis research demonstrated the possibility that waiting might have positive effects on healthcare satisfaction. It also identified variables that could produce greater positive perceptions during hospital waiting and underlying mechanisms that could explain how the positive effects work. This research may potentially help hospitals with a better understanding of how they can improve patients’ waiting experiences and increase satisfaction.
Cryptoliquidity: the blockchain and monetary stability
Authors: Caton James Lee 
Published: October 2019

PurposeThe development of blockchain and cryptocurrency may alleviate the economic strain associated with recession. Economic recessions tend to be aggregate-demand driven, meaning that they are caused by fluctuations in the supply of or demand for money. Holding monetary policy as solution assumes that stability must arise from outside of the economic system. Under a policy regime that allows innovations in blockchain to develop, blockchain technology may promote a money supply that is responsive to changes in demand to hold money. The purpose of this paper is to suggest that cryptocurrencies present an opportunity to profitably implement rules that promote macroeconomic stability. In particular, cryptocurrency that is asset-backed may provide a means for cheaply attaining liquidity during a crisis.Design/methodology/approachThe role of cryptocurrency in promoting macroeconomic equilibrium is approached through the lens of monetary theory. Moves away from macroeconomic equilibrium necessitate either a change in the average price of money or a change in the quantity of money, or a change in portfolio demand for money. Cryptocurrency promotes an increase, however this requires the alignment of policy regulating the use of cryptocurrency, reduction in taxes placed on the use of cryptocurrency and cryptocurrency protocol.FindingsCryptocurrency is unlikely to become legal tender, but it may alleviate macroeconomic fluctuations as a near money that provides liquidity and whose supply is sensitive to changes in demand to hold money and money-like substitutes. This role might be inhibited if policy stifles the development of cryptocurrencies and blockchain technology.Research limitations/implicationsNew financial innovations like cryptocurrencies can be analyzed applying the equation of exchange in light of the mechanics of money creation under conditions of disequilibrium. Monetary disequilibrium may be promoted by policy that causes bottlenecks in financial markets.Originality/valueTheory of monetary disequilibrium has broad implications for the development and regulation of financial markets. This theory has not been applied to the development of cryptocurrency markets.
Japanese guidelines for the management of pemphigoid (including epidermolysis bullosa acquisita)
Authors: Ujiie Hideyuki , Iwata Hiroaki , Yamagami Jun , Nakama Takekuni , Aoyama Yumi , Ikeda Shigaku , Ishii Norito , Iwatsuki Keiji , Kurosawa Michiko , Sawamura Daisuke , Tanikawa Akiko , Tsuruta Daisuke , Nishie Wataru , Fujimoto Wataru , Amagai Masayuki , Shimizu Hiroshi ,   Committee for Guidelines for the Management of Pemphigoid Diseases (Including Epidermolysis Bullosa Acquisita)
Published: October 2019

AbstractThe pemphigoid group is a category of autoimmune subepidermal blistering diseases in which autoantibodies deposit linearly at the epidermal basement membrane zone (BMZ). The main subtypes of pemphigoid mediated by immunoglobulin G autoantibodies are bullous pemphigoid (BP), mucous membrane pemphigoid (MMP) and epidermolysis bullosa acquisita (EBA). To establish the first guidelines approved by the Japanese Dermatological Association for the management of pemphigoid diseases, the Committee for Guidelines for the Management of Pemphigoid Diseases (Including EBA) was founded as part of the Study Group for Rare Intractable Skin Diseases under the Ministry of Health, Labor and Welfare Research Project on Overcoming Intractable Diseases. These guidelines aim to provide current information for the management of BP, MMP and EBA in Japan. Based on evidence, the guidelines summarize the clinical and immunological manifestations, pathophysiologies, diagnostic criteria, disease severity determination criteria, treatment algorithms and treatment recommendations. Because of the rarity of these diseases, there are few clinical studies with a high degree of evidence, so several parts of these guidelines were established based on the opinions of the Committee. To further optimize these guidelines, periodic revision in line with the new evidence is necessary.
Review article: fungal alterations in inflammatory bowel diseases
Authors: Lam Siu , Zuo Tao , Ho Martin , Chan Francis K. L. , Chan Paul K. S. , Ng Siew C. 
Published: October 2019

SummaryBackgroundEmerging data suggest that alterations in gut fungi may be associated with the pathogenesis of inflammatory bowel disease (IBD). In healthy individuals, gut commensal fungi act synergistically with other members of the microbiota to maintain homeostasis but their role in IBD is less clear.AimTo review the role of gut fungi and their trans‐kingdom interactions with bacteria in IBDMethodsA literature search was conducted on Ovid and Pubmed to select relevant animal and human studies that have reported fungi and IBD.ResultsThere is an increased total fungal load particularly of Candida and Malassezia species in the faeces and mucosa of Crohn's disease patients, and a lower fungal diversity in the faeces of ulcerative colitis patients. Caspase recruitment domain‐containing protein (CARD)‐9 polymorphism in Crohn's disease patients favours Malassezia colonisation that worsens gut inflammation. Diet high in carbohydrates increased the total abundance of Candida species, whereas protein‐rich diet had the opposite effect. Anti‐fungal therapies are mostly used to treat Candida albicans or Histoplasma capsulatum infections in IBD, whereas pilot studies of supplementing fungal probiotics Saccharomycopsis fibuligera, Saccharomyces boulardii and Saccharomyces cerevisiae CNCM I‐3856 strain showed therapeutic effects in IBD.ConclusionsGut fungi are altered in patients with Crohn's disease and ulcerative colitis. Modulation of the fungal microbiota can be considered as a therapeutic approach for IBD. Future research should focus on understanding how the fungal microbiota interacts with other components of the gut microbiota in association with the pathogenesis and development of IBD.
Insulin resistance: Relationship between indices during late gestation in dairy cows and effects on newborn metabolism
Authors: Hasegawa Rui , Iwase Izumi , Takagi Tomohiro , Kondo Moeri , Matsui Motozumi , Kawashima Chiho 
Published: October 2019

AbstractTo investigate the relationship between insulin resistance indices [“Revised quantitative insulin sensitivity check index” (RQUICKI; RQ), “Revised quantitative insulin sensitivity check index ‐ β‐hydroxybutyrate” (RQUICKIBHB; RQBHB), and “Homeostasis model assessment‐insulin resistance” (HOMA‐IR; HR)], and metabolic parameters in dams during late gestation, and their newborn calves. Blood was sampled twice weekly during the experimental period in 30 dry Holstein cows. In calves, blood sampling and body weight measurements were performed immediately after birth, and in 1‐week‐old male calves, liver and muscle biopsy samples were obtained for determining metabolic factor mRNA levels. RQ and RQBHB were negatively correlated with insulin, nonesterified fatty acid, BHB, and albumin and were positively correlated with leptin levels in blood during late gestation (p < .05). RQ, rather than RQBHB, reflected metabolism of dams, while stronger positive correlations were present between HR and blood insulin concentrations than other parameters, and calves of dams with high HR had low body weight, and high liver and muscle expression of growth hormone and insulin receptor mRNA (p < .05). RQ and HR of dams during late gestation could serve as indicators of dam metabolism and predictors of metabolism in newborn calves respectively.
Dynamic modeling of multivariate dimensions and their temporal relationships using latent processes: Application to Alzheimer's disease
Authors: Taddé Bachirou O. , Jacqmin‐Gadda Hélène , Dartigues Jean‐François , Commenges Daniel , Proust‐Lima Cécile 
Published: October 2019

AbstractAlzheimer's disease gradually affects several components including the cerebral dimension with brain atrophies, the cognitive dimension with a decline in various functions, and the functional dimension with impairment in the daily living activities. Understanding how such dimensions interconnect is crucial for Alzheimer's disease research. However, it requires to simultaneously capture the dynamic and multidimensional aspects and to explore temporal relationships between dimensions. We propose an original dynamic structural model that accounts for all these features. The model defines dimensions as latent processes and combines a multivariate linear mixed model and a system of difference equations to model trajectories and temporal relationships between latent processes in finely discrete time. Dimensions are simultaneously related to their observed (possibly multivariate) markers through nonlinear equations of observation. Parameters are estimated in the maximum likelihood framework enjoying a closed form for the likelihood. We demonstrate in a simulation study that this dynamic model in discrete time benefits the same causal interpretation of temporal relationships as models defined in continuous time as long as the discretization step remains small. The model is then applied to the data of the Alzheimer's Disease Neuroimaging Initiative. Three longitudinal dimensions (cerebral anatomy, cognitive ability, and functional autonomy) measured by six markers are analyzed, and their temporal structure is contrasted between different clinical stages of Alzheimer's disease.
Isoprenaline protects intestinal stem cells from chemotherapy‐induced damage
Authors: Zeng Huihong , Li Huan , Yue Mengzhen , Fan Ying , Cheng Jiaoqi , Wu Xincheng , Xu Rui , Yang Wuping , Li Manjun , Tang Jiahui , Chen Hongping , Kuang Bohai , Fan Guangqin , Zhu Qingxian , Shao Lijian 
Published: October 2019

Background and PurposeDamage to intestinal epithelial cells and mucosa limits the effectiveness of several anti‐cancer chemotherapeutic agents but the underlying mechanism (s) remain unknown. Little is known of how enteric nervous system regulates proliferation, differentiation, impairment, and regeneration of intestinal stem cells. Here we have investigated the effects of isoprenaline on the damaged intestinal stem cells induced by chemotherapeutic treatments in mice.Experimental ApproachThe effects of inhibiting sympathetic and parasympathetic nerves on intestinal stem cells were examined in male C57BL/6J mice. Protection by isoprenaline of intestinal stem cells was assessed in the presence or absence of 5‐fluorouracil (5FU) or cisplatin. Cellular apoptosis, cell cycle, PI3K/Akt signalling, and NF‐κB signalling in intestinal stem cells were mechanistically evaluated.Key ResultsThe sympathetic nerve inhibitor 6‐OHDA decreased the number and function of intestinal stem cells. 5FU or cisplatin treatment damaged both intestinal stem cells and sympathetic nerves. Notably, isoprenaline accelerated the recovery of intestinal stem cells after 5FU or cisplatin treatment. This protective effect of isoprenaline on damaged intestinal stem cells was mediated by β2‐adrenoceptors. The benefits of isoprenaline were mainly mediated by inhibiting cellular apoptosis induced by 5FU treatment, which might contribute to fine‐tuning regulating NF‐κB signalling pathway by isoprenaline administration.Conclusions and ImplicationsTreatment with isoprenaline is a new approach to ameliorate the damage to intestinal stem cells following exposure to cancer chemotherapeutic agents.
The enhanced immunopharmacology of VIB4920, a novel Tn3 fusion protein and CD40L antagonist, and assessment of its safety profile in cynomolgus monkeys
Authors: Nicholson Simone M. , Casey Kerry A. , Gunsior Michele , Drabic Stacey , Iverson William , Cook Halie , Scott Stephen , O'Day Terry , Karanth Subramanya , Dixit Rakesh , Ryan Patricia C. 
Published: October 2019

Background and PurposeInhibition of the T‐ and B‐cell interaction through the CD40/CD40 ligand (L) axis is a favourable approach for inflammatory disease treatment. Clinical studies of anti‐CD40L molecules in autoimmune diseases have met challenges because of thromboembolic events and adverse haemostasis. VIB4920 (formerly MEDI4920) is a novel CD40L antagonist and Tn3 fusion protein designed to prevent adverse haemostasis and immunopharmacology. We evaluated the pharmacokinetics, activity and toxicity of VIB4920 in monkeys.Experimental ApproachCynomolgus monkeys received i.v. or s.c. 5–300 mg·kg−1 VIB4920 or vehicle, once weekly for 1 month (Studies 1 and 2) or 28 weeks (Study 3). VIB4920 exposure and bioavailability were determined using pharmacokinetic analyses, and immune cell population changes via flow cytometry. Pharmacological activity was evaluated by measuring the animals' capacity to elicit an immune response to keyhole limpet haemocyanin (KLH) and tetanus toxoid (TT).Key ResultsVIB4920 demonstrated linear pharmacokinetics at multiple doses. Lymphocyte, monocyte, cytotoxic T‐cell and NK cell counts were not significantly different between treatment groups. B‐cell counts reduced dose‐dependently and the T‐cell dependent antibody response to KLH was suppressed by VIB4920 dose‐dependently. The recall response to TT was similar across treatment groups. No thromboembolic events or symptoms of immune system dysfunctionality were observed.Conclusions and ImplicationsVIB4920 demonstrated an acceptable safety profile in monkeys. VIB4920 showed favourable pharmacokinetics, dose‐dependent inhibition of a neoantigen‐specific immune response and no adverse effects on immune function following long‐term use. Our data support the use of VIB4920 in clinical trials.
“If they misbehaved, we took a stick to discipline them”: Refugee mothers' struggles raising children in the United States
Authors: Kohli Hermeet K. , Fineran Susan 
Published: October 2019

AbstractAlthough there are compelling examples of stress put upon refugee families, there is little systematic attention placed upon how the refugee women themselves view child‐rearing. In this exploratory research, refugee women's narratives concerning their understanding and perceptions of child‐rearing in the United States are studied. Seventeen refugee women raising children living in the Greater Portland region in Maine were interviewed. While resettling in the United States, the participants shared being overburdened by the loss of social structures that supported their parenting beliefs and strategies back home. They perpetually encounter cultural conflicts in the United States that result in a continuous process of contradictory interpretations and negotiations while rearing and disciplining children. Recommendations are provided for service providers working with this population. Professionals have to be cognizant of the hierarchical decision‐making and retributive strategies used by refugee families when disciplining children. Tools, that is, explaining, teaching, and rewarding children for coping with parenting challenges without resorting to punitive discipline methods can be introduced to refugee families. Professionals need to focus on providing refugee families with basic needs and addressing social and emotional challenges so that they can better integrate with the new communities as they rebuild their lives.
Collaboration and conversations with children in Child Welfare Services —Parents' viewpoint
Authors: Juul Randi , Husby Inger Sofie Dahlø 
Published: October 2019

AbstractCollaboration and conversations are important in meeting vulnerable children's needs in the context of Child Welfare Services (CWS). Building on 10 qualitative interviews with parents of children in Norwegian Child Welfare Services, this paper discusses parents' views on collaboration between children and child welfare professionals. The parents stated that a constructive collaborative relationship depends on professionals' attitudes towards the child, their ability to connect with the child and their awareness of how the child's emotions and how the parents influence the child–professional relationship. A collaborative relationship is essential for child welfare professionals to meet the child's needs and to help improve relations between the child and the parents. The parents asked for more collaboration between children and child welfare professionals. The findings call for more discussion of child welfare workers' tasks and competence.