bluuebunny/crossref_metadata_2025_split huggingface dataset:
Barium chloride injures myofibers through calcium-induced proteolysis with fragmentation of motor nerves and microvessels
Authors: Morton Aaron B. , Norton Charles E. , Jacobsen Nicole L. , Fernando Charmain A. , Cornelison D. D. W. , Segal Steven S. 
Published: November 2019

AbstractBackgroundLocal injection of BaCl2is an established model of acute injury to study the regeneration of skeletal muscle. However, the mechanism by which BaCl2causes muscle injury is unresolved. Because Ba2+inhibits K+channels, we hypothesized that BaCl2induces myofiber depolarization leading to Ca2+overload, proteolysis, and membrane disruption. While BaCl2spares resident satellite cells, its effect on other tissue components integral to contractile function has not been defined. We therefore asked whether motor nerves and microvessels, which control and supply myofibers, are injured by BaCl2treatment.MethodsThe intact extensor digitorum longus (EDL) muscle was isolated from male mice (aged 3–4 months) and irrigated with physiological salt solution (PSS) at 37 °C. Myofiber membrane potential (Vm) was recorded using sharp microelectrodes while intracellular calcium concentration ([Ca2+]i) was evaluated with Fura 2 dye. Isometric force production of EDL was measured in situ, proteolytic activity was quantified by calpain degradation of αII-spectrin, and membrane disruption was marked by nuclear staining with propidium iodide (PI). To test for effects on motor nerves and microvessels, tibialis anterior or gluteus maximus muscles were injected with 1.2% BaCl2(50–75 μL) in vivo followed by immunostaining to evaluate the integrity of respective tissue elements post injury. Data were analyzed using Studentsttest and analysis of variance withP ≤ 0.05 considered statistically significant.ResultsAddition of 1.2% BaCl2to PSS depolarized myofibers from − 79 ± 3 mV to − 17 ± 7 mV with a corresponding rise in [Ca2+]i; isometric force transiently increased from 7.4 ± 0.1 g to 11.1 ± 0.4 g. Following 1 h of BaCl2exposure, 92 ± 3% of myonuclei stained with PI (vs. 8 ± 3% in controls) with enhanced cleavage of αII-spectrin. Eliminating Ca2+from PSS prevented the rise in [Ca2+]iand ameliorated myonuclear staining with PI during BaCl2exposure. Motor axons and capillary networks appeared fragmented within 24 h following injection of 1.2% BaCl2and morphological integrity deteriorated through 72 h.ConclusionsBaCl2injures myofibers through depolarization of the sarcolemma, causing Ca2+overload with transient contraction, leading to proteolysis and membrane rupture. Motor innervation and capillarity appear disrupted concomitant with myofiber damage, further compromising muscle integrity.
On the asymptotic analysis of bounded solutions to nonlinear differential equations of second order
Authors: Tunç Cemil , Mohammed Sizar Abid 
Published: November 2019

Abstract
In this paper, we consider two different models of nonlinear ordinary differential equations (ODEs) of second order. We construct two new Lyapunov functions to investigate boundedness of solutions of those nonlinear ODEs of second order. By using the Lyapunov direct or second method and inequality techniques, we prove two new theorems on the boundedness solutions of those ODEs of second order as $t \to \infty $t→∞. When we compare the conditions of the theorems of this paper with those of Meng in (J. Syst. Sci. Math. Sci. 15(1):50–57, 1995) and Sun and Meng in (Ann. Differ. Equ. 18(1):58–64 2002), we can see that our theorems have less restrictive conditions than those in (Meng in J. Syst. Sci. Math. Sci. 15(1):50–57, 1995) and Sun and Meng in (Ann. Differ. Equ. 18(1):58–64 2002) because of the two new suitable Lyapunov functions. Next, in spite of the use of the Lyapunov second method here and in (Meng in J. Syst. Sci. Math. Sci. 15(1):50–57, 1995; Sun and Meng in Ann. Differ. Equ. 18(1):58–64 2002), the proofs of the results of this paper are proceeded in a very different way from that used in the literature for the qualitative analysis of ODEs of second order. Two examples are given to show the applicability of our results. At the end, we can conclude that the results of this paper generalize and improve the results of Meng in (J. Syst. Sci. Math. Sci. 15(1):50–57, 1995), Sun and Meng in (Ann. Differ. Equ. 18(1):58–64 2002), and some other that can be found in the literature, and they have less restrictive conditions than those in these references.
Assessment of land use change and its effect on soil carbon stock using multitemporal satellite data in semiarid region of Rajasthan, India
Authors: Sharma Garima , Sharma L. K. , Sharma K. C. 
Published: November 2019

Abstract

Background
Land use change plays a vital role in global carbon dynamics. Understanding land use change impact on soil carbon stock is crucial for implementing land use management to increase carbon stock and reducing carbon emission. Therefore, the objective of our study was to determine land use change and to assess its effect on soil carbon stock in semi-arid part of Rajasthan, India. Landsat temporal satellite data of Pushkar valley region of Rajasthan acquired on 1993, 2003, and 2014 were analyzed to assess land use change. Internal trading of land use was depicted through matrices. Soil organic carbon (SOC) stock was calculated for soil to a depth of 30 cm in each land use type in 2014 using field data collection. The SOC stock for previous years was estimated using stock change factor. The effect of land use change on SOC stock was determined by calculating change in SOC stock (t/ha) by deducting the base-year SOC stock from the final year stock of a particular land use conversion.

Results
The total area under agricultural lands was increased by 32.14% while that under forest was decreased by 23.14% during the time period of 1993–2014. Overall land use change shows that in both the periods (1993–2003 and 2003–2014), 7% of forest area was converted to agricultural land and about 15% changes occurred among agricultural land. In 1993–2003, changes among agricultural land led to maximum loss of soil carbon, i.e., 4.88 Mt C and during 2003–2014, conversion of forest to agricultural land led to loss in 3.16 Mt C.

Conclusion
There was a continuous decrease in forest area and increase in cultivated area in each time period. Land use change led to alteration in carbon equity in soil due to change or loss in vegetation. Overall, we can conclude that the internal trading of land use area during the 10-year period (1993–2003) led to net loss of SOC stock by 8.29 Mt C. Similarly, land use change during 11-year period (2003–2014) caused net loss of SOC by 2.76 Mt C. Efforts should be made to implement proper land use management practices to enhance the SOC content.
Natural occurrence of aflatoxins and ochratoxin A in meju and soybean paste produced in South Korea
Authors: Jeong Seong Eun , Chung Soo Hyun , Hong Sung-Yong 
Published: November 2019

Abstract
In this study, we investigated the occurrence of aflatoxins (AFs) and ochratoxin A (OTA) in meju and soybean paste produced in South Korea. Samples were collected from three regions divided on the basis of climate in South Korea. A total of 100 meju samples were analyzed over 3 years (2012–2015), and 45 soybean paste samples were analyzed in 2016. Mycotoxins were extracted with an immunoaffinity column method and quantified by high-performance liquid chromatography. AFs were detected in 10 of meju (10%) and 11 of soybean paste samples (24.4%) with concentrations of 0.2–48.3 μg/kg and 0.88–16.17 μg/kg, respectively. OTA was detected in 50 of meju (50%) and 22 of soybean paste samples (48.9%) with concentrations of 0.1–193.2 μg/kg and 0.88–26.29 μg/kg, respectively. Mycotoxin contamination in meju was more common in the central region than in the southern areas. Thus, more mycotoxins were produced in the central region owing to less fungal competition in meju during fermentation inside households. We also found that about 91% of AFs and 73% of OTA in meju were degraded after the production of soybean paste and soy sauce. Even after degradation of AFs and OTA, the levels of AFB1 and OTA were 0.5 µg/kg and 7.5 µg/kg in soy sauce and 11.9 µg/kg and 190.4 µg/kg in soybean paste, respectively. Thus, our results suggest the need for constant monitoring of meju and soybean paste for AFs and OTA.
Identification of single nucleotide polymorphisms of PIK3R1 and DUSP1 genes and their genetic associations with milk production traits in dairy cows
Authors: Han Bo , Yuan Yuwei , Shi Lijun , Li Yanhua , Liu Lin , Sun Dongxiao 
Published: November 2019

Abstract

Background
Previously, phosphoinositide-3-kinase regulatory subunit 1 (PIK3R1) and dual specificity phosphatase 1 (DUSP1) were identified as promising candidate genes for milk production traits due to their being differentially expressed between the dry period and the peak of lactation in livers of dairy cows. Hence, in this study, the single nucleotide polymorphisms (SNPs) of PIK3R1 and DUSP1 genes were identified and their genetic associations with milk yield, fat yield, fat percentage, protein yield, and protein percentage, were investigated using 1067 Chinese Holstein cows from 40 sire families.

Results
By re-sequencing the entire coding region and 2000 bp of the 5′ and 3′ flanking regions of the two genes, one SNP in the 5′ untranslated region (UTR), three in the 3′ UTR, and two in the 3′ flanking region of PIK3R1 were identified, and one in the 5′ flanking region, one in the 3′ UTR, and two in the 3′ flanking region of DUSP1 were found. Subsequent single-locus association analyses showed that five SNPs in PIK3R1, rs42590258, rs210389799, rs208819656, rs41255622, rs133655926, and rs211408208, and four SNPs in DUSP1, rs207593520, rs208460068, rs209154772, and rs210000760, were significantly associated with milk, fat and protein yields in the first or second lactation (P values ≤ 0.0001 and 0.0461). In addition, by the Haploview 4.2 software, the six and four SNPs in PIK3R1 and DUSP1 respectively formed one haplotype block, and the haplotype-based association analyses showed significant associations between their haplotype combinations and the milk traits in both two lactations (P values ≤ 0.0001 and 0.0364). One SNP, rs207593520(T/G), was predicted to alter the transcription factor binding sites (TFBSs) in the 5′ flanking region of DUSP1. Further, the dual-luciferase assay showed that the transcription activity of allele T in rs207593520 was significantly higher than that of allele G, suggesting the activation of transcriptional activity of DUSP1 gene by allele T of rs207593520. Thus, the rs207593520 SNP was highlighted as a potential causal mutation that should be further verified.

Conclusions
We demonstrated novel and significant genetic effects of the PIK3R1 and DUSP1 genes on milk production traits in dairy cows, and our findings provide information for use in dairy cattle breeding.
The efficacy of a resilience-enhancement program for mothers in Japan based on emotion regulation: study protocol for a randomized controlled trial
Authors: Tobe Hiromi , Sakka Mariko , Kamibeppu Kiyoko 
Published: November 2019

Abstract

Background
The demands of daily life often cause mothers high levels of distress and other negative emotions. Anger, including harsh verbal discipline, has been linked to child maltreatment, with long-term adverse effects on a child’s well-being. It is critically important to teach mothers stress management and emotion regulation in addition to parenting skills, but this is yet to be conducted in a formalized manner. Strengthening the multiple protective factors that constitute resilience helps reduce distress. The aim of this study is to evaluate the efficacy of a resilience-enhancement program for mothers.

Methods
We designed a two-arm, parallel, randomized trial with an active control. Mothers and their partners with children between three and six years old will be recruited. Following an online baseline survey, 140 mothers will be randomly allocated to either an intervention or control group. Self-report assessment will be conducted online post-intervention and at a two-month follow-up. The control group will participate in a serious of group discussions. The intervention group will participate in four bi-weekly 120-min sessions of a Cognitive Behavior Therapy-based program designed to enhance resilience, focusing on emotion regulation through cognitive reappraisal. Participants will be encouraged to apply and share the skills they acquire with their partner and children at home. Partners will also be assessed to explore their indirect influence from the mothers. Intention-to-treat analysis will be conducted and the two groups will be compared, applying covariate analysis. The primary outcome of the intervention is improved resilience. Secondary outcomes include improved anger control, self-esteem, cognition of children’s misbehavior, and reduced parental stress.

Discussion
To the best of our knowledge, this study will evaluate the first resilience-enhancement program focused on emotion regulation for mothers in Japan. It will contribute to the existing body of knowledge on building emotional resilience. If the program is found to be effective, it will provide an alternative means to enhance mothers’ resilience against stress and improve their ability to regulate emotion. In so doing, it will offer a way to prevent child maltreatment and protect the mental health of children and families.

Trial registration
UMIN000027232, May 3, 2017.
Multiplex proteomics identifies novel CSF and plasma biomarkers of early Alzheimer’s disease
Authors: Whelan Christopher D. , Mattsson Niklas , Nagle Michael W. , Vijayaraghavan Swetha , Hyde Craig , Janelidze Shorena , Stomrud Erik , Lee Julie , Fitz Lori , Samad Tarek A. , Ramaswamy Gayathri , Margolin Richard A. , Malarstig Anders , Hansson Oskar 
Published: November 2019

Abstract
To date, the development of disease-modifying therapies for Alzheimer’s disease (AD) has largely focused on the removal of amyloid beta Aβ fragments from the CNS. Proteomic profiling of patient fluids may help identify novel therapeutic targets and biomarkers associated with AD pathology. Here, we applied the Olink™ ProSeek immunoassay to measure 270 CSF and plasma proteins across 415 Aβ- negative cognitively normal individuals (Aβ- CN), 142 Aβ-positive CN (Aβ+ CN), 50 Aβ- mild cognitive impairment (MCI) patients, 75 Aβ+ MCI patients, and 161 Aβ+ AD patients from the Swedish BioFINDER study. A validation cohort included 59 Aβ- CN, 23 Aβ- + CN, 44 Aβ- MCI and 53 Aβ+ MCI. To compare protein concentrations in patients versus controls, we applied multiple linear regressions adjusting for age, gender, medications, smoking and mean subject-level protein concentration, and corrected findings for false discovery rate (FDR, q < 0.05). We identified, and replicated, altered levels of ten CSF proteins in Aβ+ individuals, including CHIT1, SMOC2, MMP-10, LDLR, CD200, EIF4EBP1, ALCAM, RGMB, tPA and STAMBP (− 0.14 < d < 1.16; q < 0.05). We also identified and replicated alterations of six plasma proteins in Aβ+ individuals OSM, MMP-9, HAGH, CD200, AXIN1, and uPA (− 0.77 < d < 1.28; q < 0.05). Multiple analytes associated with cognitive performance and cortical thickness (q < 0.05). Plasma biomarkers could distinguish AD dementia (AUC = 0.94, 95% CI = 0.87–0.98) and prodromal AD (AUC = 0.78, 95% CI = 0.68–0.87) from CN. These findings reemphasize the contributions of immune markers, phospholipids, angiogenic proteins and other biomarkers downstream of, and potentially orthogonal to, Aβ- and tau in AD, and identify candidate biomarkers for earlier detection of neurodegeneration.
A comprehensive protocol for ventilator weaning and extubation: a prospective observational study
Authors: Nitta Kenichi , Okamoto Kazufumi , Imamura Hiroshi , Mochizuki Katsunori , Takayama Hiroshi , Kamijo Hiroshi , Okada Mayumi , Takeshige Kanako , Kashima Yuichiro , Satou Takahisa 
Published: November 2019

Abstract

Background
Ventilator weaning protocols have been shown to reduce the duration of mechanical ventilation (MV), intensive care unit length of stay, and resource use. However, weaning protocols have not significantly affected mortality or reintubation rates. The extubation process is a critical component of respiratory care in patients who receive MV. Post-extubation respiratory failure (PERF) is a common event associated with significant morbidity and mortality. We hypothesized that a comprehensive protocol for ventilator weaning and extubation would be effective for preventing PERF and reintubation and reducing mortality in critically ill patients.

Methods
A ventilator weaning and extubation protocol was developed. The protocol consisted of checklists across four evaluations: spontaneous breathing trial, extubation, prophylactic non-invasive positive pressure ventilation (NPPV), and evaluation after extubation. Observational data were collected after implementing the protocol in patients admitted to the Advanced Emergency and Critical Care Center of Shinshu University Hospital. Not only outcomes of patients but also influences of each component of the protocol on the clinical decision-making process were investigated. Further, a comparison between PERF and non-PERF patients was performed.

Results
A total of 464 consecutive patients received MV for more than 48 h, and 248 (77 women; mean age, 65 ± 17 years) were deemed eligible. The overall PERF and reintubation rates were 9.7% and 5.2%, respectively. Overall, 54.1% of patients with PERF received reintubation. Hospital stay and mortality were not significantly different between PERF and non-PERF patients (p = 0.16 and 0.057, respectively). As a result, the 28-day and hospital mortality were 1.2% and 6.9%, respectively.

Conclusions
We found that the rates of PERF, reintubation, and hospital mortality were lower than those in previous reports even with nearly the same degree of severity at extubation. The comprehensive protocol for ventilator weaning and extubation may prevent PERF and reintubation and reduce mortality in critically ill patients.
Influence of bimanual exercise on muscle activation in post-stroke patients
Authors: Itkonen Matti , Costa Álvaro , Yamasaki Hiroshi , Okajima Shotaro , Alnajjar Fady , Kumada Takatsune , Shimoda Shingo 
Published: November 2019

Abstract
Brain damage due to stroke often leaves survivors with lateral functional motor deficits. Bimanual rehabilitation of the paretic arm is an active field of research aimed at restoring normal functionality by making use of the complex neural bindings that exist between the arms. In search of an effective rehabilitation method, we introduced a group of post-stroke rehabilitation patients to a set of bimanual motion tasks with inter-manual coupling and phasing. The surface EMG profiles of the patients were compared in order to understand the effect of the motion conditions. The paretic arms of the patients were more strongly affected by the task conditions compared with the non-paretic arms. These results suggest that in-phase motion may activate neural circuits that trigger recovery. Coupling also had an effect on behavior, but the response of patients was divided between those whom coupling helped or hindered.
An insight analysis and detection of drug-abuse risk behavior on Twitter with self-taught deep learning
Authors: Hu Han , Phan NhatHai , Chun Soon A. , Geller James , Vo Huy , Ye Xinyue , Jin Ruoming , Ding Kele , Kenne Deric , Dou Dejing 
Published: November 2019

Abstract
Drug abuse continues to accelerate towards becoming the most severe public health problem in the United States. The ability to detect drug-abuse risk behavior at a population scale, such as among the population of Twitter users, can help us to monitor the trend of drug-abuse incidents. Unfortunately, traditional methods do not effectively detect drug-abuse risk behavior, given tweets. This is because: (1) tweets usually are noisy and sparse and (2) the availability of labeled data is limited. To address these challenging problems, we propose a deep self-taught learning system to detect and monitor drug-abuse risk behaviors in the Twitter sphere, by leveraging a large amount of unlabeled data. Our models automatically augment annotated data: (i) to improve the classification performance and (ii) to capture the evolving picture of drug abuse on online social media. Our extensive experiments have been conducted on three million drug-abuse-related tweets with geo-location information. Results show that our approach is highly effective in detecting drug-abuse risk behaviors.
Biomass and dominance of conservative species drive above-ground biomass productivity in a mediterranean-type forest of Chile
Authors: Ayma-Romay Ariel Isaías , Bown Horacio E. 
Published: November 2019

Abstract

Background
Forest productivity has a pivotal role in human well-being. Vegetation quantity, niche complementarity, mass-ratio, and soil resources are alternative/complementary ecological mechanisms driving productivity. One challenge in current forest management depends on identifying and manipulating these mechanisms to enhance productivity. This study assessed the extent to which these mechanisms control above-ground biomass productivity (AGBP) of a Chilean mediterranean-type matorral. AGBP measured as tree above-ground biomass changes over a 7-years period, was estimated for twelve 25 m × 25 m plots across a wide range of matorral compositions and structures. Variables related to canopy structure, species and functional diversity, species and functional dominance, soil texture, soil water and soil nitrogen content were measured as surrogates of the four mechanisms proposed. Linear regression models were used to test the hypotheses. A multimodel inference based on the Akaike’s information criterion was used to select the best models explaining AGBP and for identifying the relative importance of each mechanism.

Results
Vegetation quantity (tree density) and mass-ratio (relative biomass of Cryptocarya alba, a conservative species) were the strongest drivers increasing AGBP, while niche complementarity (richness species) and soil resources (sand, %) had a smaller effect either decreasing or increasing AGBP, respectively. This study provides the first assessment of alternative mechanisms driving AGBP in mediterranean forests of Chile. There is strong evidence suggesting that the vegetation quantity and mass-ratio mechanisms are key drivers of AGBP, such as in other tropical and temperate forests. However, in contrast with other studies from mediterranean-type forests, our results show a negative effect of species diversity and a small effect of soil resources on AGBP.

Conclusion
AGBP in the Chilean matorral depends mainly on the vegetation quantity and mass-ratio mechanisms. The findings of this study have implications for matorral restoration and management for the production of timber and non-timber products and carbon sequestration.