bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bromodomain and Extraterminal Inhibition by JQ1 Produces Divergent Transcriptional Regulation of Suppressors of Cytokine Signaling Genes in Adipocytes
Authors: Mota de Sá Paula , Richard Allison J , Stephens Jacqueline M 
Published: December 2019

Abstract
The Janus kinase-signal transducer and activator of transcription (JAK-STAT) signaling pathway has cell-specific functions. Suppressors of cytokine signaling (SOCS) proteins are negative-feedback regulators of JAK-STAT signaling. STAT5 plays a significant role in adipocyte development and function, and bromodomain and extraterminal (BET) proteins may be involved in STAT5 transcriptional activity. We treated 3T3-L1 adipocytes with the BET inhibitor JQ1 and observed that growth hormone (GH)-induced expression of 2 STAT5 target genes from the SOCS family, Socs3 and Cish, were inversely regulated (increased and decreased, respectively) by BET inhibition. Chromatin immunoprecipitation analyses revealed that changes in STAT5 binding did not correlate with gene expression changes. GH promoted the recruitment of the BET protein BRD2 to the Cish, but not Socs3, promoter. JQ1 treatment ablated this effect as well as the GH-induced binding of ribonucleic acid polymerase II (RNA Pol II) to the Cish transcription start site. BRD2 knockdown also suppressed GH induction of Cish, further supporting the role of BRD2 in Cish transcriptional activation. In contrast, JQ1 increased the binding of activated Pol II to the Socs3 coding region, suggesting enhanced messenger RNA (mRNA) elongation. Our finding that JQ1 transiently reduced the interaction between the positive transcription elongation factor (P-TEFb) and its inhibitor hexamethylene bis-acetamide inducible 1 (HEXIM1) is consistent with a previously described off-target effect of JQ1, whereby P-TEFb becomes more available to be recruited by genes that do not depend on BET proteins for activating transcription. These results demonstrate substantially different transcriptional regulation of Socs3 and Cish and suggest distinct roles in adipocytes for these 2 closely related proteins.
The Autophagy Gene Atg16L1 is Necessary for Endometrial Decidualization
Authors: Oestreich Arin K , Chadchan Sangappa B , Popli Pooja , Medvedeva Alexandra , Rowen Marina N , Stephens Claire S , Xu Ran , Lydon John P , Demayo Francesco J , Jungheim Emily S , Moley Kelle H , Kommagani Ramakrishna 
Published: December 2019

Abstract
Uterine receptivity is critical for establishing and maintaining pregnancy. For the endometrium to become receptive, stromal cells must differentiate into decidual cells capable of secreting factors necessary for embryo survival and placental development. Although there are multiple reports of autophagy induction correlated with endometrial stromal cell (ESC) decidualization, the role of autophagy in decidualization has remained elusive. To determine the role of autophagy in decidualization, we utilized 2 genetic models carrying mutations to the autophagy gene Atg16L1. Although the hypomorphic Atg16L1 mouse was fertile and displayed proper decidualization, conditional knockout in the reproductive tract of female mice reduced fertility by decreasing the implantation rate. In the absence of Atg16L1, ESCs failed to properly decidualize and fewer blastocysts were able to implant. Additionally, small interfering RNA knock down of Atg16L1 was detrimental to the decidualization response of human ESCs. We conclude that Atg16L1 is necessary for decidualization, implantation, and overall fertility in mice. Furthermore, considering its requirement for human endometrial decidualization, these data suggest Atg16L1 may be a potential mediator of implantation success in women.
Injured Runners Do Not Replace Lost Running Time with Other Physical Activity
Authors: DAVIS JOHN J. , GRUBER ALLISON H. 
Published: December 2019

ABSTRACTRunning-related injuries are common and may pose a barrier to maintaining high levels of overall physical activity.PurposeThe aim of this study was to determine whether recreational runners remain physically active while experiencing running-related pain or running-related injury.MethodsRecreational runners (n= 49) participated in a year-long observational cohort study. Subjects were issued a commercial activity monitor to measure daily physical activity level, quantified by the total minutes of moderate to vigorous physical activity (MVPA). Subjects also completed a weekly survey inquiring about running-related pain and any modifications made to planned running sessions. A week was classified as an “injured week” if a runner reported a reduction or cancellation of at least three planned training sessions, otherwise, it was categorized as an “uninjured week.” Separately, pain level was assessed for each week using a 0 to 10 scale. Survey responses were used to longitudinally track pain levels and injury status for each runner. Mixed-effect linear models were used to quantify whether sustaining an injury or reporting running-related pain during a given week were associated with changes in MVPA levels for that week.ResultsCompared with uninjured weeks, runners engaged in 14.1 fewer minutes of MVPA per day (95% confidence interval, −22.5 to −6.0) during weeks in which they reported a running-related injury. Lost MVPA during injured weeks was primarily replaced by sedentary activity. There was no significant association between running-related pain and MVPA.ConclusionsInjured runners do not replace lost running time with other forms of MVPA. Running-related injury and running-related pain should not be conflated; although reporting injury is associated with a reduction in physical activity in recreational runners, high pain levels are not.
Endurance Athletes Are at Increased Risk for Early Acute Mountain Sickness at 3450 m
Authors: SAREBAN MAHDI , SCHIEFER LISA M. , MACHOLZ FRANZISKA , SCHÄFER LARISSA , ZANGL QUIRIN , INAMA FRANCISCUS , REICH BERNHARD , MAYR BARBARA , SCHMIDT PETER , HARTL ARNULF , BÄRTSCH PETER , NIEBAUER JOSEF , TREFF GUNNAR , BERGER MARC M. 
Published: December 2019

ABSTRACT

Introduction
Acute mountain sickness (AMS) may develop in nonacclimatized individuals after exposure to altitudes ≥2500 m. Anecdotal reports suggest that endurance-trained (ET) athletes with a high maximal oxygen uptake (V˙O2max) may be at increased risk for AMS. Possible underlying mechanisms include a training-induced increase in resting parasympathetic activity, higher resting metabolic rate (RMR), and lower hypoxic ventilatory response (HVR).

Methods
In 38 healthy, nonacclimatized men (19 ET and 19 untrained controls [UT], V˙O2max 66 ± 6 mL·min−1·kg−1 vs 45 ± 7 mL·min−1·kg−1; P < 0.001) peripheral oxygen saturation (SpO2), heart rate variability, RMR, and poikilocapnic HVR were assessed at 424 m and during 48 h at 3450 m after passive ascent by train (~2 h). Acute mountain sickness was evaluated by AMS cerebral (AMS-C) score.

Results
On day 1 at altitude, ET presented with a higher AMS incidence (42% vs 11%; P < 0.05) and severity (AMS-C score: ET, 0.48 ± 0.5 vs UT, 0.21 ± 0.2; P = 0.03), but no group difference was found on days 2 and 3. SpO2 decreased upon arrival at altitude (ET: 82% ± 6% vs UT: 83% ± 4%; p
time <0.001) with a significantly different time course between ET and UT (p
time × group = 0.045). Parasympathetic activity decreased at altitude (P < 0.001) but was always higher in ET (P < 0.05). At altitude RMR increased (P < 0.001) and was higher in ET (P < 0.001). Hypoxic ventilatory response increased only in ET (P < 0.05) and was greater than in UT after 24 and 48 h (P < 0.05).

Conclusions
Endurance-trained athletes are at higher risk for developing AMS on the first day after passive and rapid ascent to 3450 m, possibly due to an increased parasympathetic activity and an increased RMR, while HVR appeared to be of minor importance. Differences in AMS time course and physiological responses should be taken into consideration when ET are planning high-altitude sojourns.
Physical Activity, Sedentary Behavior, and White Matter Microstructure in Children with Overweight or Obesity
Authors: RODRIGUEZ-AYLLON MARÍA , Esteban-Cornejo IRENE , VERDEJO-ROMÁN JUAN , Muetzel RYAN L. , Migueles JAIRO H. , Mora-Gonzalez JOSE , Solis-Urra PATRICIO , Erickson KIRK I. , Hillman CHARLES H. , Catena ANDRÉS , Tiemeier HENNING , Ortega FRANCISCO B. 
Published: December 2019

ABSTRACTPurposeThis study aimed to investigate the associations of objectively measured and self-reported physical activity (PA) and sedentary behavior with white matter microstructure in children with overweight or obesity.MethodsIn a sample of 103 participants (age, 10.02 ± 1.15 yr; 42 girls) from the ActiveBrains project, we assessed PA and sedentary behavior using accelerometers (GT3X+; ActiGraph, Pensacola, FL), and the Youth Activity Profile-Spain questionnaire. Objectively measured PA and sedentary behavior were classified into different intensities following the hip- and wrist-based cutoff points for the Euclidean Norm Minus One metric by Hildebrand et al., wrist-based cutoff points for counts metric by Chandler et al., and hip-based cutoff points for counts metric for Romanzini et al. and Evenson et al. Magnetic resonance imaging of white matter microstructure, fractional anisotropy (FA), and mean diffusivity (MD) were derived from diffusion tensor imaging. Linear regression models were used to examine the associations of objectively measured and self-reported PA and sedentary behavior with global and tract-specific FA and MD.ResultsSelf-reported total PA was positively associated with global FA (β = 0.236,P= 0.038), whereas watching television was negatively associated with global FA (β = −0.270,P= 0.014). In regard to the objective measures, using single regression models, light PA (β = 0.273,P= 0.016), moderate-to-vigorous PA (β = 0.257,P= 0.035), and total PA (β = 0.294,P= 0.013) were positively associated with global FA only when Hildebrand–Euclidean Norm Minus One hip cut points were used for analyses. Lastly, no association was found between PA and sedentary behavior and FA and MD within individual tracts.ConclusionsOur results suggest that PA and watching television are related to white matter microstructure in children with overweight or obesity. However, longitudinal large-scale studies are needed to confirm and expand these findings.
Teaching Object Play to Young Children With Disabilities: A Systematic Review of Methods and Rigor
Authors: Barton Erin E. , Murray Rebecca , O'Flaherty Cecelia , Sweeney Erin M. , Gossett Stephanie 
Published: December 2019

Abstract
In this systematic review, we examined the rigor and outcomes across 27 object play intervention studies using single-case research methodology. We focused on studies including children age 5 years or younger and examined several descriptive characteristics including materials, instructional packages, and settings. We also analyzed the facilitation and measurement of generalized play and several methodological features including quality, rigor, and visual analysis procedures. Overall, the identified studies demonstrated positive outcomes, although quality and rigor limited interpretations of the outcomes. Previous reviews also have noted strong outcomes and weak to moderate quality for single-case studies. Our results should be interpreted with caution given previous reviews of play intervention studies identified strong outcomes and quality from group-design studies. Additional replications testing robust interventions using single-case research with strong methodological rigor are warranted.
Associated Risk Factors for Depression and Anxiety in Adults With Intellectual and Developmental Disabilities: Five-Year Follow Up
Authors: Hsieh Kelly , Scott Haleigh M. , Murthy Sumithra 
Published: December 2019

Abstract
A better understanding of the factors associated with depression and anxiety in people with intellectual and developmental disabilities (IDD) is needed to provide guidelines for service providers, clinicians, and researchers as well as to improve the diagnostic process. The current study used a longitudinal dataset to explore demographic, health, and psychosocial risk factors of anxiety and depression in adults with IDD. Women were more likely to have depression while older adults, people with autism, and people with hearing impairments, were more likely to have anxiety. Chronic health conditions were associated with both anxiety and depression, while changes in stressful life events were associated with an increased risk of anxiety. Clinical and research contributions are discussed.
Preliminary Characterization of Parent-Child Interaction in Preschoolers With Prader-Willi Syndrome: The Relationship Between Engagement and Parental Stress
Authors: Zyga Olena , Dimitropoulos Anastasia 
Published: December 2019

Abstract
Early parent-child interactions (PCI) impact social cognitive development. Relatedly, children with various developmental disorders exhibit abnormal parental attachment relationships. Parental characteristics and behaviors can impact PCI and socioemotional development as well. No research has examined the parent-child dynamic in Prader-Willi syndrome (PWS), a neurodevelopmental disorder that presents with social cognitive deficits. This article provides a preliminary characterization of PCI quality and parenting stress in 17 PWS parent-child dyads, children ages 3–5 years, in comparison to 20 typically developing children and their parent. Results suggest early PCI disruption in preschoolers with PWS and their parents report increased levels of stress in various domains. These findings have important implications not only on parent well-being in PWS but its impact on child development.
PENGEMBANGAN KURIKULUM PENDIDIKAN AGAMA ISLAM DAN IMPLEMENTASINYA DI MTS NURUL UMMAH YOGYAKARTA
Authors: Sugiana Aset 
Published: December 2019

This study aims to: describe the implementation of the PAI curriculum in the subjects of Aqeedah Akhlaq. This is a qualitative research. The research data were obtained from Aqeedah Akhlaq teacher in implementing PAI curriculum. Data analysis technique used is classifying, combining, interpreting, and concluding. The results of research on the development of the PAI curriculum: 1) Focusing on the development potential, needs, and interests of students and their environment, 2) Diverse and integrated, 3) Responsiveness to the development of science, technology, and art, 4) Relevant to the needs of life, 5) Comprehensive and continuous, 6) Lifelong learning, 7) Balance between national interests and regional interests. Implementation at MTs Nurul Ummah Yogyakarta, namely: 1) Organizing Workshops or socialization about making lesson plans, 2) Using textbooks from the Ministry of Religion and also pesantren (The Yellow Book), 3) Learning Aqeedah Akhlaq focuses on three aspects: cognitive, affective, and psychomotor, 4) Using the nahwu amtsilati learning method from Jepara and curriculum integration from Purworejo.
Abstrak
Penelitian bertujuan untuk: mendeskripsikan tentang implementasi kurikulum PAI pada mata pelajaran Aqidah Akhlaq. Metode penelitian ini menggunakan penelitian kualitatif. Data penelitian diperoleh dari hasil pencatatan dari guru Aqidah Akhlaq dalam mengimplementasikan kurikulum PAI. Teknik analisis data yang digunakan yaitu mengklasifikasikan, menggabungkan, menafsirkan, dan menyimpulkan. Hasil penelitian tentang pengembangan kurikulum PAI: 1) Berpusat pada potensi perkembangan, kebutuhan, dan kepentingan peserta didik dan lingkungannya, 2) Beragam dan terpadu, 3) Tanggap terhadap perkembangan ilmu pengetahuan, teknologi, dan seni, 4) Relevan dengan kebutuhan kehidupan, 5) Menyeluruh dan berkesinambungan, 6) Belajar sepanjang hayat, 7) Seimbang antara kepentingan nasional dan kepentingan daerah. Implementasi di MTs Nurul Ummah Yogyakarta, yaitu: 1) Mengadakan Workshop atau mensosialisasikan tentang pembuatan RPP, 2) Menggunakan buku paket dari Kemenag dan juga kitab pesantren (Kitab Kuning), 3) Dalam pembelajaran PAI Aqidah Akhlaq pada tiga aspek kognitif, afektif, dan psikomotorik, dan 4) Menggunakan metode pembelajaran nahwu amtsilati dari Jepara dan integrasi kurikulum dari Purworejo.
Effect of gas adsorption-induced pore radius and effective stress on shale gas permeability in slip flow: New Insights
Authors: Memon Asadullah , Li Aifen , Han Wencheng , Tian Weibing 
Published: December 2019

Abstract
Shale, a heterogeneous and extremely complex gas reservoir, contains low porosity and ultra-Low permeability properties at different pore scales. Its flow behaviors are more complicated due to different forms of flow regimes under laboratory conditions. Flow regimes change with respect to pore scale variation resulting in change in gas permeability. This work presents new insights regarding the change of pore radius due to gas adsorption, effective stress and impact of both on shale gas permeability measurements in flow regimes. From this study, it was revealed that the value of Klinkenberg coefficient has been affected due to gas adsorption-induced pore radius thickness impacts and resulting change in gas permeability. The gas permeability measured from new proposed equation is provides better results as compare to existing equation. Adsorption parameters are the key factors that affect radius of shale pore. Both adsorption and effective stress have an effect on the pore radius and result gas permeability change. It was found that slip effect enhances the apparent gas permeability and also changes with effective stress; therefore, combine impact of slip flow and effective stress is very important as provides understanding in evolution of apparent permeability during shale gas production.
Beta Generalized Exponentiated Frechet Distribution with Applications
Authors: Badr Majdah M. 
Published: December 2019

Abstract
In this article we introduce a new six - parameters model called the Beta Generalized Exponentiated-Frechet (BGEF) distribution which exhibits decreasing hazard rate. Many models such as Beta Frechet (BF), Beta ExponentiatedFrechet (BEF), Generalized Exponentiated-Frechet (GEF), ExponentiatedFrechet (EF), Frechet (F) are sub models. Some of its properties including rth
moment, reliability and hazard rate are investigated. The method of maximum likelihood isproposed to estimate the model parameters. The observed Fisher’s information matrix is given. Moreover, we give the advantage of the (BGEF) distribution by an application using two real datasets
Discrimination, Stress and Reactivity to Alcohol Cues
Authors: Stepanova Elena V. , Echevarria David J. , Collier Adam D. , Cruz Cristobal S. , Kirkland Nafiyah , Drobes David J. 
Published: December 2019

Introduction: Our study investigated whether discrimination affects psychologi-cal/physiological stress and alcohol craving.Method: Participants (N = 92) were asked to recall and write about (a) a neutral, (b) a negative, or (c) a discriminatory experience in the past and then completed a cue-reactivity procedure assessing their alcohol craving. In addition, we assessed levels of perceived stress before and after the discrimination manipulation, chronic substance use and craving, prior perceived discrimination, and strength of racial/ethnic identity.Results: Results revealed a small effect in which the discrimination condition increased alcohol cue-elicited craving relative to the other conditions. Chronic craving moderated effects of discrimination on cortisol levels. Self-reported stress levels were increased in the discrimination and negative memory conditions relative to baseline. Strength of racial identity served as a protective factor for substance abuse in those who reported chronic high levels of discrimination.Discussion: We discussed the direct link established between acute exposure to discrimination and craving, and experimental evidence for the relationship between discrimination and self-reported stress, but also addressed potential limitations of this work. It is further discussed how some individual differences factors (e.g., chronic craving) predict physiological stress in discriminatory settings. This work underscored the role of racial identity as a protective factor against alcohol abuse in individuals reporting high levels of discrimination.
Dyadic Adjustment and Prenatal Parental Depression: A Study with Expectant Mothers and Fathers
Authors: Mangialavori Sonia , Terrone Grazia , Cantiano Arianna , Chiara Franquillo Anna , Di Scalea Giulia Lanza , Ducci Giuseppe , Cacioppo Marco 
Published: December 2019

Introduction: This paper evaluated the relationships between the dyadic adjustment of expectant parents and prenatal maternal and paternal depression.Method: Participants were 98 couples who were expectant parents in the third trimester of pregnancy. Most couples (97%) were primiparous. Participants' prenatal depression, psychiatric symptomatology, perinatal affectivity, and dyadic adjustment were evaluated.Results: Hierarchical regression and relative weight analyses showed the importance of various marital adjustment dimensions in predicting prenatal maternal and paternal depression. In particular, the marital relationship variables of dyadic consensus and affective expression of both partners was related to prenatal depression in expectant mothers, with the relationship even stronger in expectant fathers. The results suggested that for both partners, perception of marital relationship quality contributes to the development of depressive symptoms in new mothers and fathers to a greater degree than the single perception of one partner.Discussion: Clinically, the results suggest that clinicians should focus on partner relationships in the perinatal period. The provision of psychological interventions to improve a couple's functioning may help to protect new parents against depressive symptomatology.
Blue Light-Induced Gene Expression Alterations in Cultured Neurons Are the Result of Phototoxic Interactions with Neuronal Culture Media
Authors: Duke Corey G. , Savell Katherine E. , Tuscher Jennifer J. , Phillips Robert A. , Day Jeremy J. 
Published: December 2019

AbstractBlue wavelength light is used as an optical actuator in numerous optogenetic technologies employed in neuronal systems. However, the potential side effects of blue light in neurons has not been thoroughly explored, and recent reports suggest that neuronal exposure to blue light can induce transcriptional alterationsin vitroandin vivo. Here, we examined the effects of blue wavelength light in cultured primary rat cortical cells. Exposure to blue light (470 nm) resulted in upregulation of several immediate early genes (IEGs) traditionally used as markers of neuronal activity, includingFosandFosb, but did not alter the expression of circadian clock genesBmal1,Cry1,Cry2,Clock, orPer2. IEG expression was increased following 4 h of 5% duty cycle light exposure, and IEG induction was not dependent on light pulse width. Elevated levels of blue light exposure induced a loss of cell viabilityin vitro, suggestive of overt phototoxicity. Induction of IEGs by blue light was maintained in cortical cultures treated with AraC to block glial proliferation, indicating that induction occurred selectively in postmitotic neurons. Importantly, changes in gene expression induced by blue wavelength light were prevented when cultures were maintained in a photoinert media supplemented with a photostable neuronal supplement instead of commonly utilized neuronal culture media and supplements. Together, these findings suggest that light-induced gene expression alterations observedin vitrostem from a phototoxic interaction between commonly used media and neurons, and offer a solution to prevent this toxicity when using photoactivatable technologyin vitro.