bluuebunny/crossref_metadata_2025_split huggingface dataset:
Targeted lung denervation in sheep: durability of denervation and long-term histologic effects on bronchial wall and peribronchial structures
Authors: Mayse Martin L. , Norman Holly S. , Peterson Alexander D. , Rouw Kristina T. , Johnson Philip J. 
Published: May 2020

Abstract
Background
Targeted lung denervation (TLD), a novel bronchoscopic procedure which attenuates pulmonary nerve input to the lung to reduce the clinical consequences of neural hyperactivity, may be an important emerging treatment for COPD. While procedural safety and impact on clinical outcomes have recently been reported, the mechanism of action has not been reported. We explored the long-term pathologic and histopathologic effects in a sheep model of ablation of bronchial branches of the vagus nerve using a novel dual-cooled radiofrequency ablation catheter.

Methods
Nineteen sheep underwent circumferential ablation of both main bronchi with simultaneous balloon surface cooling using a targeted lung denervation system (Nuvaira, Inc., USA). Animals were followed over an extended time course (30, 365, and 640 days post procedure). At each time point, lung denervation (axonal staining in bronchial nerves), and effect on peribronchial structures near the treatment site (histopathology of bronchial epithelium, bronchial cartilage, smooth muscle, alveolar parenchyma, and esophagus) were quantified. One way analysis of variance (ANOVA) was performed to reveal differences between group means on normal data. Non-parametric analysis using Kruskal-Wallis Test was employed on non-normal data sets.

Results
No adverse clinical effects were observed in any sheep. Nerve axon staining distal to the ablation site was decreased by 60% at 30 days after TLD and efferent axon staining was decreased by >70% at 365 and 640 days. All treated airways exhibited 100% epithelial integrity. Effect on peribronchial structures was strictly limited to lung tissue immediately adjacent to the ablation site. Tissue structure 1 cm proximal and distal to the treatment area remained normal, and the pulmonary veins, pulmonary arteries, and esophagus were unaffected.

Conclusions
The denervation of efferent axons induced by TLD therapy is durable and likely a contributing mechanism through which targeted lung denervation impacts clinical outcomes. Further, long term lung denervation did not alter the anatomy of the bronchioles or lung, as evaluated from both a gross and histologic perspective.
Association between parent and child physical activity: a systematic review
Authors: Petersen Therese Lockenwitz , Møller Liselotte Bang , Brønd Jan Christian , Jepsen Randi , Grøntved Anders 
Published: May 2020

AbstractBackgroundChildhood represents an important life stage for establishment of physical activity (PA) habits. Parents are assumed to play an important role in influencing children’s PA. Earlier reviews have mainly focused on parental modelling, encouragement, and support for PA, rather than the actual PA levels of parents. Therefore, the purpose of this review was to systematically summarize the evidence on the relationship between parent and child PA.MethodsPapers were identified using electronic databases and manual searches of reference lists. Papers reporting on associations between objectively measured child PA and at least one measure of parental PA were included. The quality of the papers was assessed using a modified version of the ROBINS-I tool. For interpretation of the results across studies, we produced albatross plots for all studies combined and by age-groups, sex of the parents, sex of the child, methodology of assessment of parental PA, and type of PA.ResultsThirty-nine papers were included with sample size of parent-child dyads ranging from 15 to 1267 (mean = 319 dyads, median = 227 dyads). The majority of studies were published from 2008 to 2018 and used accelerometry to assess PA. Most of the studies were classified as having moderate, serious, or critical risk of bias. The albatross plot for all studies combined showed that the clear majority of studies observed a positive relationship between parent and child PA. The plot suggested an average magnitude of correlation across studies to be around 0.13, and the overall impression was that this was fairly similar across child age-groups and gender of parent-child dyads. Studies using objective assessment of parental PA showed stronger relationship between parent and child PA compared with studies using self-report (average magnitude of correlation around 0.16 vs 0.04 respectively). No clear evidence was found for the strength of relationship being dependent on type of PA measure of parent and child (total PA, moderate-to-vigorous PA, steps), however, the relationship for light PA appeared weaker.ConclusionThis systematic review showed that the clear majority of studies observed a weak positive relationship between parent and child PA regardless of age of the child, the gender of the parent-child dyad, and type of PA.Trial registrationRegistration in PROSPERO:CRD42019093462.
A Qualitative Exploration of the Determinants of Exclusive Breastfeeding (EBF) Practices in Wajir County, Kenya
Authors: Mohamed Mahat Jimale , Ochola Sophie , Owino Victor O. 
Published: May 2020

AbstractBackgroundThe World Health Organization recommends exclusive breastfeeding for the first six months of life. A qualitative study was conducted to assess the factors that influence the practice of exclusive breastfeeding amongst mothers attending Wajir County Hospital, Kenya.MethodThis study was part of a cross-sectional study to compare the exclusive breastfeeding rates amongst primiparous and multiparous mothers with infants under 6 months old attending Wajir County Hospital. Focus group discussions and key informant interviews were conducted to collect information on exclusive breastfeeding and related factors. Four focus group discussions were conducted with mothers who exclusively breastfed and the same number with mothers who did not exclusively breastfeed their babies. Key informant interviews were conducted with nine healthcare providers. The data were transcribed, and a content analysis identified common themes and inferences.ResultsThe exclusive breastfeeding rate among the mothers in the larger study was 45.5%. There was no disparity between the practice of exclusive breastfeeding between primiparous and multiparous mothers. Despite the high knowledge and positive attitudes towards exclusive breastfeeding of most mothers, the practice of exclusive breastfeeding was unsatisfactory. The major hindrances identified were cultural barriers propagated by mothers-in-law and traditional birth attendants; the belief that babies cannot live without water; and a few unsupportive health workers. The uptake of exclusive breastfeeding was enhanced by Islamic teaching on breastfeeding, education from a few supportive healthcare providers; support from husbands; and positive deviance among some lactating mothers who practiced exclusive breastfeeding.ConclusionsDeeply rooted cultural factors were the major hindrance to the practice of exclusive breastfeeding. Most of the mothers did not practice exclusive breastfeeding, despite the majority being knowledgeable and having positive attitudes towards the practice. The influence of mother-in-law’s and traditional birth assistants were major barriers. Strengthening the Community Health Strategy through training traditional birth attendants on Infant Young Child Nutrition practices, designing mechanisms linking traditional birth assistants to existing health facilities for support, and capacity building and monitoring is critical in promoting exclusive breastfeeding. Behavior change and communication through multiple channels within the community should be utilized to maximize promotion of exclusive breastfeeding among all stakeholders.
Nutrient solutions for Arabidopsis thaliana: a study on nutrient solution composition in hydroponics systems
Authors: van Delden Sander H. , Nazarideljou Mohammad Javad , Marcelis Leo F. M. 
Published: May 2020

Abstract
Background
There is little information on the effect of nutrient solutions composition on Arabidopsis growth. Therefore, we compared growth performance of Arabidopsis thaliana (Col-0) grown on the most commonly used nutrient solutions in deep water culture: Hoagland and Arnon, Murashige and Skoog, Tocquin, Hermans, and Conn. In addition to these nutrient solution composition experiments, we established Arabidopsis growth response curves for nutrient solution concentration and salt stress (NaCl).

Results
Arabidopsis rosette fresh and dry weight showed an approximate linear decline with NaCl dose in deep water culture, i.e. 9% reduction relative to control per unit of electrical conductivity (EC in dS m−1, for scale comprehension 1 dS m−1 equals ~ 10 mM NaCl). The Tocquin, ½Hoagland and Conn nutrient solutions had equal and optimal growth performance. Optimal nutrient solution concentration for Tocquin and Hoagland was 0.8 to 0.9 dS m−1. Close to the EC of ½Hoagland (1.1 dS m−1), which is frequently used in Arabidopsis research. Conn solution showed optimal growth at much higher EC (2 dS m−1) indicating that it is a balanced nutrient solution that matches the needs of Arabidopsis. Full Murashige and Skoog solution (5.9 dS m−1) was lethal and diluted solutions (EC of 1.6 and 1.1 dS m−1) caused stress symptoms and severe growth retardation at later developmental stages.

Conclusions
Arabidopsis thaliana (Col-0) plants grown in deep water culture showed a sixfold growth difference when commonly used nutrient solutions were compared. Murashige and Skoog solution should not be used as nutrient solution in deep water culture. Conn, Tocquin and ½Hoagland are balanced nutrient solutions which result in optimal Arabidopsis growth in hydroponic systems.
Efficacy and safety of Onkyeong-tang in treating cold hypersensitivity in the feet of Korean women: protocol for a double-blind, randomized, placebo-controlled, parallel-group, multicenter clinical study
Authors: Lee Kyou-Young , Han In-Sik , Go Ho-Yeon , Lee Dong-Nyung , Yu Jun-Sang , Sun Seung-Ho 
Published: May 2020

Abstract
Background
Cold hypersensitivity in the hands and feet (CHHF) commonly affects Asian women, especially Korean women, and it negatively impacts the quality of life of the affected individuals. One commonly used herbal prescription for treating CHHF is Onkyeong-tang (OKT). Although OKT is widely used clinically in treating CHHF, no randomized clinical trial has been performed to evaluate the efficacy and safety of OKT in the treatment of cold hypersensitivity in the feet (CHF). This clinical trial aims to provide objective evidence for the basis of using OKT in the treatment of CHF in Korean women.

Methods
This trial will be a double-blind, randomized, placebo-controlled, parallel-group, multicenter pilot study. A total of 112 participants will be randomly divided into an OKT treatment group or a placebo group with a 1:1 ratio via a web-based randomization system. The OKT and placebo groups will receive prescribed medications orally three times per day (3 g each time) before or between meals for 8 weeks. The primary outcome studied will be the changes in Visual Analog Scale (VAS) scores of CHF from baseline. Secondary outcomes studied will be the VAS score changes of cold hypersensitivity in the hands, changes in the skin temperature of the hands and feet, total scores of the Korean version of the World Health Organization Quality of Life Scale-abbreviated version, and the results of the cold stress test.

Discussion
This trial will be the first clinical trial to assess the efficacy and safety of OKT in the treatment of CHF. We anticipate that the findings of the study will provide evidence for the basis of using OKT in treating CHF symptoms and generate basic data for designing a further large-scale randomized clinical trial.

Trial registration
Clinical Research Information Service (CRIS): KCT0003723. Retrospectively registered on 8 April 2019.
Is it time to use real-world data from primary care in Alzheimer’s disease?
Authors: Ponjoan Anna , Garre-Olmo Josep , Blanch Jordi , Fages Ester , Alves-Cabratosa Lia , Martí-Lluch Ruth , Comas-Cufí Marc , Parramon Dídac , Garcia-Gil María , Ramos Rafel 
Published: May 2020

Abstract
Background
The analysis of real-world data in clinical research is rising, but its use to study dementia subtypes has been hardly addressed. We hypothesized that real-world data might be a powerful tool to update AD epidemiology at a lower cost than face-to-face studies, to estimate the prevalence and incidence rates of AD in Catalonia (Southern Europe), and to assess the adequacy of real-world data routinely collected in primary care settings for epidemiological research on AD.

Methods
We obtained data from the System for the Development of Research in Primary Care (SIDIAP) database, which contains anonymized information of > 80% of the Catalan population. We estimated crude and standardized incidence rates and prevalences (95% confidence intervals (CI)) of AD in people aged at least 65 years living in Catalonia in 2016.

Results
Age- and sex-standardized prevalence and incidence rate of AD were 3.1% (95%CI 2.7–3.6) and 4.2 per 1000 person-years (95%CI 3.8–4.6), respectively. Prevalence and incidence were higher in women and in the oldest people.

Conclusions
Our incidence and prevalence estimations were slightly lower than the recent face-to-face studies conducted in Spain and higher than other analyses of electronic health data from other European populations. Real-world data routinely collected in primary care settings could be a powerful tool to study the epidemiology of AD.
An improved content-based outlier detection method for ICS intrusion detection
Authors: Li Huiping , Wang Bin , Xie Xin 
Published: May 2020

AbstractDue to the complexity of industrial control systems and the diversity of protocols in networks, it is difficult to build intrusion detection models based on network characteristics and physical modeling. In order to build a better flow model without additional knowledge, we propose an intrusion detection method based on the content of network packets. The construction of the model is based on the idea of ZOE method. The similarity between flows is calculated through the sequential coverage algorithm, the normal flow model is established by multi-layered clustering algorithm, and the Count-Mean-Min Sketch is used to store and count the flow model. By comparing the unknown flow with the constructed normal flow model, we achieve the intrusion detection of industrial control system (ICS). The overall experimental results on 4 ICS datasets show that the improved method can effectively improve the detection rate and reduce the false-positive rate. The detection rate reached 96.7% on average, and the false-positive rate reached 0.7% on average.
Tax4Fun2: prediction of habitat-specific functional profiles and functional redundancy based on 16S rRNA gene sequences
Authors: Wemheuer Franziska , Taylor Jessica A. , Daniel Rolf , Johnston Emma , Meinicke Peter , Thomas Torsten , Wemheuer Bernd 
Published: May 2020

Abstract
Background
Sequencing of 16S rRNA genes has become a powerful technique to study microbial communities and their responses towards changing environmental conditions in various ecosystems. Several tools have been developed for the prediction of functional profiles from 16S rRNA gene sequencing data, because numerous questions in ecosystem ecology require knowledge of community functions in addition to taxonomic composition. However, the accuracy of these tools relies on functional information derived from genomes available in public databases, which are often not representative of the microorganisms present in the studied ecosystem. In addition, there is also a lack of tools to predict functional gene redundancy in microbial communities.

Results
To address these challenges, we developed Tax4Fun2, an R package for the prediction of functional profiles and functional gene redundancies of prokaryotic communities from 16S rRNA gene sequences. We demonstrate that functional profiles predicted by Tax4Fun2 are highly correlated to functional profiles derived from metagenomes of the same samples. We further show that Tax4Fun2 has higher accuracies than PICRUSt and Tax4Fun. By incorporating user-defined, habitat-specific genomic information, the accuracy and robustness of predicted functional profiles is substantially enhanced. In addition, functional gene redundancies predicted with Tax4Fun2 are highly correlated to functional gene redundancies determined for simulated microbial communities.

Conclusions
Tax4Fun2 provides researchers with a unique tool to predict and investigate functional profiles of prokaryotic communities based on 16S rRNA gene sequencing data. It is easy-to-use, platform-independent and highly memory-efficient, thus enabling researchers without extensive bioinformatics knowledge or access to high-performance clusters to predict functional profiles. Another unique feature of Tax4Fun2 is that it allows researchers to calculate the redundancy of specific functions, which is a potentially important measure of how resilient a community will be to environmental perturbation. Tax4Fun2 is implemented in R and freely available at https://github.com/bwemheu/Tax4Fun2.
The long-term course of fatigue following breast cancer diagnosis
Authors: Biering Karin , Frydenberg Morten , Pappot Helle , Hjollund Niels Henrik 
Published: May 2020

Abstract
Purpose
Fatigue following breast cancer is a well-known problem, with both high and persistent prevalence. Previous studies suffer from lack of repeated measurements, late recruitment and short periods of follow-up. The course of fatigue from diagnosis and treatment to the long-time outcome status is unknown as well as differences in the level of fatigue between treatment regimens. The purpose of this study was to describe the long-time course of fatigue from the time of clinical suspicion of breast cancer, its dependence of patient characteristics and treatment regimens and the comparison with the course of fatigue among women with the same suspicion, but not diagnosed with breast cancer.

Methods
Three hundred thirty-two women referred to acute or subacute mammography was followed with questionnaires from before the mammography and up to 1500 days. Fatigue was measured by the Multidimensional Fatigue Inventory (MFI-20). The women reported their initial level of fatigue before the mammography and thus without knowledge of whether they had cancer or not. Both women with and without cancer were followed. Women with cancer were identified in the clinical database established by Danish Breast Cancer Cooperative Group (DBCG) to collect information on treatment regimen.

Results
Compared to fatigue scores before diagnosis, women with breast cancer reported a large increase of fatigue, especially in the first 6 months, followed by a slow decrease over time. Despite the long follow-up period, the women with breast cancer did not return to their level of fatigue at time of the mammography. Women without breast cancer, experienced a rapid decrease of fatigue after disproval of diagnosis followed by a steadier period.

Conclusions
Fatigue is a persistent problem in women diagnosed with breast cancer, even several years following diagnosis and treatment. The women with breast cancer were most affected by fatigue in the first 6 months after diagnosis.
Morphological, molecular, and pathogenicity characteristics of the native isolates of Metarhizium anisopliae against the tomato leafminer, Tuta absoluta (Meyrick 1917) (Lepidoptera: Gelechiidae) in Ethiopia
Authors: Ayele Birhan Aynalem , Muleta Diriba , Venegas Juan , Assefa Fassil 
Published: May 2020

AbstractThe South American tomato leafminer,Tuta absoluta(Meyrick 1917) (Lepidoptera: Gelechiidae), is a newly introduced and a major threat of tomato production in Ethiopia. Laboratory bioassay was conducted to evaluate locally isolatedMetarhizium anisopliae(Metsch.) (Sorokin), against larvae ofT. absoluta. Twenty-fiveMetarhiziumisolates were isolated from different soil types, using the great wax moth,Galleria mellonellaL. baiting method. From these, 13 isolates were prescreened by biological efficiency index model and tested against 2nd and 3rd larval instars ofT. absolutaat the concentration of 107spores ml−1. Sterile water plus Tween 80 (0.1% v/v) was used as a control. Greenhouse reared larvae ofT. absolutawere used as experimental organisms. All testedMetarhiziumformulations were pathogenic toT. absolutain all conducted bioassays. Three isolates, AAUM78, AAUM39, and AAUM76, were the most effective and scored 88, 90, and 95% and 90, 93.3, and 95.7% mortality against 2nd and 3rd larval instars ofT. absoluta, respectively. The lowest (48.5 and 50%) mortality rate was recorded by isolate AAUM30 against 2nd and 3rd larval instars, respectively, in 7 days post inoculation. However, all isolates showed significant statistical differences withF(24, 50) = 6.825,p< 0.001 andF(23, 15) = 3.97,p< 0.001 of mortality to 2nd and 3rd larval instars, respectively. Molecular analysis categorized these selected isolates under species ofM. anisopliaeand grouped into 2 different groups. AAUM78 and AAUM39 were recorded LT50values of 3.93 and 3.5 days at the lowest (104spore ml−1) concentration and LC50values of 1.2 × 103and 2.9 × 103spore ml−1, respectively. Although AAUM78 and AAUM39 were virulent to the target pest, further field evaluation is required to determine their potential efficacy forT. absolutacontrol.
Serum progranulin/tumor necrosis factor-α ratio as independent predictor of systolic blood pressure in overweight hypertensive patients: a cross-sectional study
Authors: Kaur Jasmine , Mukheja Supriya , Varma Sudhir , Kalra Harpreet Singh , Khosa Bipanjeet Singh , Vohra Kanchan 
Published: May 2020

AbstractBackgroundVascular inflammation plays a key role in the progression of hypertension. Progranulin (PGRN), an anti-inflammatory growth factor, mediated inhibition of tumor necrosis factor-α (TNF-α), a pleiotropic cytokine, activity has been well-established. Despite the role of chronic low-grade inflammation in hypertension, serum levels of PGRN and PGRN/TNF-α ratio and, their association with systolic and diastolic blood pressure has not been determined in hypertensive patients till now. This study aims to find and correlate the serum levels of pro-inflammatory cytokine (TNF-α), anti-inflammatory growth factor (PGRN), and PGRN/TNF-α ratio with the blood pressure in systolic-diastolic hypertension (SDH) and isolated systolic hypertension (ISH) patients.ResultsA cross-sectional study was conducted on SDH patients (mean age, 52.95 ± 12.6 years; male/female (M/F) number = 15/10) and ISH patients (mean age, 55.80 ± 9.40 years; M/F number = 12/13) (n= 25 each). Twenty-five age and body mass index (BMI)-matched healthy subjects (mean age, 56.00 ± 8.55 years; male/female number = 11/14) were considered as control. All patients and healthy subjects were overweight (BMI, 25–30 kg/m2). Overnight fasting blood samples of subjects were taken and levels of PGRN and TNF-α were measured using ELISA diagnostic kits. PGRN and TNF-α levels were found significantly high, whereas PGRN/TNF ratio was found very low, in SDH and ISH patients as compared to healthy subjects. Reduced PGRN/TNF-α ratio and pulse pressure were found as independent predictors of SBP both in SDH and ISH patients.ConclusionsFindings of elevated PGRN levels in response to raised TNF-α levels depict the counter regulation by PGRN to neutralize TNF-α. Findings of reduced PGRN/TNF ratio, and it being an independent predictor of SBP, ascertain the key role of imbalance in pro- and anti-inflammatory environment in hypertension. Thus, it strengthens the cross-link between the concept of immunity–adiposity–inflammation–blood pressure¸ a vicious network. Further, this cross-link of SBP and progranulin must be explored in longitudinal studies. New researches should be focused not only on impact of pro-inflammatory environment rather to find on a balance between pro- and anti-inflammatory status, so that new target sites could be explored for therapeutic management of hypertension.