bluuebunny/crossref_metadata_2025_split huggingface dataset:
The&nbsp;biometric&nbsp;parameters&nbsp;of&nbsp;aniso-astigmatism&nbsp;and its risk factor in&nbsp;Chinese preschool children:&nbsp;the Nanjing Eye Study&nbsp;
Authors: Tong Haohai , Hao Qingfeng , Wang Zijin , Wang Yue , Li Rui , Zhao Xiaoyan , Sun Qigang , Zhang Xiaohan , Chen Xuejuan , Zhu Hui , Huang Dan , Liu Hu 
Published: December 2020

Abstract
Backgrounds: Aniso-astigmatism may hinder normal visual development in preschool children. Knowing its prevalence, biometric parameters and risk factors is fundamental to children eye care. The purpose of this study was to determine the biometric components of aniso-astigmatism and associated maternal risk factors in Chinese preschool children. Methods: In the population-based, prospective cohort Nanjing Eye Study, children were measured for noncycloplegic refractive error using an autorefractor and for biometric parameters using an optical low-coherent reflectometry. The difference of total astigmatism (TA) between both eyes was calculated using cylinder power (non-vectorial aniso-TA was defined as ≥1.00 Dioptre Cylinder [DC] between both eyes) and by vector analysis (vectorial aniso-TA was defined as a difference of ≥0.5 in J0 or J45 between both eyes which is equivalent to 1.00 DC). The prevalence of aniso-TA was presented. Interocular biometric parameters were compared between with vs. without aniso-astigmatism group. In addition, risk factors were determined using multivariate logistic regression model. Results: Of 1131 children (66.90±3.38 months, 53.31% male), the prevalence of non-vectorial aniso-TA was 1.95% (95% Confidence Interval (CI)=1.14%-2.75%), while the prevalence of vectorial aniso-TA was twice as common as non-vectorial aniso-TA, neither varying with sex or age. With aniso-TA eyes were more asymmetric in axial length and corneal curvature radius than without aniso-TA eyes. In multivariate logistic regression model, 5-min Apgar score less than 7 was significantly associated with higher risk of aniso-TA (vectorial aniso-TA: Odds Ratio (OR)=6.42, 95%CI=2.63-15.69, P<0.001; non-vectorial aniso-TA: OR=4.99, 95%CI=1.41-17.68, P=0.01）. Being twin or triple was significantly associated with higher risk of vectorial aniso-CA (OR=2.43, 95%CI=1.05-5.60, P=0.04). Pre-term delivery (OR=2.60, 95%CI=1.09-6.15, P=0.03) and post-term delivery (OR=3.61, 95%CI=1.31-9.96, P=0.01) were significantly associated with higher risk of vectorial aniso-CA. Conclusions: Both corneal curvature radius and axial length asymmetry were correlated with aniso-TA. Children with 5-min Apgar score<7 were more likely to have aniso-TA, while twin or triple, pre-term or post-term delivery were more likely to have vectorial aniso-CA.
Low Arterial Oxygen Partial Pressure Induces Pulmonary Thrombocytopenia in Patients and a Mouse Model.
Authors: Wu Limeng , Guo Ninghong , Xu Zhenyan , Wang Wei , Xiong Qinmei , Hu Jinzhu , Wan Rong , Hong Kui 
Published: December 2020

Abstract
Background: Recent basic studies demonstrate that the lung is a primary organ of platelet biogenesis. However, whether the pathophysiological state of the lung affect the platelets is little known. We aim to investigate the incidence of thrombocytopenia in patients with pulmonary infection (PIN) and risk factors associated with pulmonary thrombocytopenia.Methods: In total, 11941 patients with pulmonary infection (PIN) were enrolled, and patients with other three infectious diseases were collected as controls. The incidence of thrombocytopenia was compared, and the risk factors associated with thrombocytopenia in PIN patients were investigated by multivariate analysis. To explore the mechanism of thrombocytopenia, hypoxic model was constructed. Blood platelet counts from the angular vein (PLTs), left ventricle (PLTpost) and right ventricle (PLTpre) were determined. Megakaryocytes identified by anti-CD41 antibody were detected through flow cytometry and immunofluorescence.Results: The incidence of thrombocytopenia in PIN was higher than that in other three infectious diseases (9.8% vs 6.4%~5.0%, P<0.001). Low arterial oxygen partial pressure (PaO2) was an important risk factor for thrombocytopenia (OR=0.88; P<0.001). In a hypoxic mouse model, PLTs decreased (518.38±127.92 vs 840.75±77.30, P<0.05), which showed that low PaO2 induced thrombocytopenia. The difference between the PLTpost and PLTpre (△PLTpost-pre), representing the production of platelets in the lungs, was significantly attenuated in hypoxic mice when compared with normoxic mice (F=25.47, P<0.05). Additionally, proportions of CD41-positive megakaryocytes in the lungs, marrow, spleen all decreased in hypoxic mice.Conclusion: There is a high incidence for thrombocytopenia in PIN patients. Low PaO2-induced thrombocytopenia is associated with impaired generation of platelet in the lungs.
The outcomes of a person-centered, non-pharmacological intervention in reducing agitation in residents with dementia in Australian rural nursing homes
Authors: Isaac Vivian , Kuot Abraham , Hamiduzzaman Mohammad , Strivens Edward , Greenhill Jennene 
Published: December 2020

Abstract
BackgroundThere is limited best- practice evidence to address behavioral and psychiatric symptoms for those with dementia in Australian rural nursing homes. This study aims to evaluate the outcomes of a person-centered, non-pharmacological dementia care model, ‘Harmony in the Bush’, based on the Progressively Lowered Stress Threshold principles and person-centered music in rural Australia.MethodsA quasi-experimental (nonrandomized, pre-post) intervention study was conducted in five rural nursing homes in Queensland and South Australia. Seventy-four residents with dementia participated in this intervention study, which yielded a sample power of 80%. Eighty-seven staff completed the Caregiver Stress Inventory at pre-post four-weeks of intervention. Staff training workshops focused on the theory of the Progressively Lowered Stress Threshold principles and delivery of person-centered care plan with integrated music intervention. We used reported changes in agitation of the residents, measured using Cohen- Mansfield Agitation Inventory, and staff’s caregiving stress, using Caregivers Stress Inventory. This study adheres to the CONSORT guidelines.ResultsMean age of residents with dementia was 82.4 (7.7) years and 69% were females. The mean age of admission was 80.1(8.4) years. Baseline measures indicated that 32.7% had mild- severe pain and 30.5% reported mild-severe sadness. The results showed statistically significant decline in aggressive behaviors, physically non-aggressive behaviors, verbally agitated behavior and hiding and hoarding. There was similar reduction in staff stress in the domains of aggressive behaviors, inappropriate behaviors, resident safety, and resource deficiency.ConclusionsThe Harmony in the Bush model is effective in reducing agitation among dementia residents with significant reduction in staff stress levels in nursing homes in rural Australia.Trial Registration: Australian and New Zealand Clinical Trials Registry (ANZCTR) on 20/2/2018 (Registration No: ACTRN12618000263291p). https://www.anzctr.org.au/Trial/Registration/TrialReview.aspx?id=374458
Use of&nbsp;masks in public&nbsp;places in Poland&nbsp;during SARS-Cov-2 epidemic:&nbsp;&nbsp;a&nbsp;covert observational study
Authors: Ganczak Maria , Pasek Oskar , Duda-Duma lukasz , Świstara Dawid , Korzeń Marcin 
Published: December 2020

Abstract
Background: Face masks have been employed in the COVID-19 pandemic plans as a public and personal health control measure against the spread of SARS-CoV-2. In Poland, obligatory wearing of masks in public spaces was introduced on April 10th, 2020; a relaxation of previous universal measures was announced on May 29th, 2020, limiting use to indoor public places. Objective: to assess use of masks or other protective devices in public spaces in Poland during the SARS-Cov-2 epidemic. Methods: A non-participatory covert observational study was conducted on three dates, (10.05/18.05/ 25.05.2020) at public spaces in 13 regions with different risks. Ten consecutive individuals were observed by each of 82 medical students (n=2460 observations), using a structured checklist. Results: Among 2353 observed persons, the female/male ratios were 1.0, 1.1, and 1.0 on the three dates. Almost three quarters - 73.6% (n=552/750) were using masks on date 1, 66.5% (544/818) on date 2; and 65.7% (516/785) on date 3. Cloth masks predominated on all dates (64.7%-62.3%-62.6%), followed by medical (23.4%-28.5%-26.9%). Being female (OR=1.77-1.47-1.53 respectively) and location in a closed space (OR=2.60-2.59-2.32) were each associated with higher usage. Participants in sports were about two times less likely to use masks (OR=0.64-0.53-0.53) as compared to other activities. The proportion using masks correctly decreased gradually over time (364/552; 65.9%; 339/544; 62.3% and 304/516; 58.9%). More females wore masks correctly (date 1: 205/294; 69.7% vs 159/258; 61.6%, and date 3: 186/284; 65.5% vs 118/232; 50.9%; p=0.045; p=0.0008 respectively). Uncovered noses (47.3%-52.7%) and masks around the neck (39.2%-42.6%) were the most frequent incorrect practices. Conclusions: Practices were not in line with official recommendations, especially among males, and deteriorated over time. Cloth masks were predominantly used in public spaces. Health promotion, through utilizing all available communication channels, would be helpful to increase compliance.
Poor health-related physical fitness performance increases the overweight and obesity risk in elderly people from Taiwan
Authors: Ho Chien-Chang , Lee Po-Fu , Chen Hui-Ling , Tseng Ching-Yu , Hsieh Xin-Yu , Chiu Chih Hui 
Published: December 2020

Abstract
Background: The purpose of the present study was to investigate the associations between health-related physical fitness performance and overweight/obesity risk among Taiwanese healthy older adults.Methods: A secondary dataset from the nationwide survey was applied in this study. Data from a total of 21,630 respondents aged 65-96 years were collected in this study. Demographic characteristics, life habits, perceived health status, anthropometric assessments, and health-related physical fitness measurements from this dataset were analyzed using the chi-square test, one-way analysis of variance, and logistic regression analysis.Results: The results indicated that overweight and obesity significantly reduced health-related physical fitness performance in the Taiwanese older adult population. In particular, the upper extremity muscular endurance scores of older adults with poor activity and physical fitness scores revealed obesity as a critical indicator of health-related physical fitness performance.Conclusions: Future studies can use muscle quality or body fat classification to predict obesity in older adults, which could more precisely portray the relationship between obesity and health-related physical fitness performance.
Psychiatric Disorders in Female Psychosexual Disorders—A Nationwide, Cohort Study in Taiwan
Authors: Lin Iau-Jin , Tzeng Nian-Sheng , Chung Chi-Hsiang , Wu-chien Chien 
Published: December 2020

Abstract
We aimed to investigate as to whether females with psychosexual disorders were associated with the risk of affective and other psychiatric disorders. A total of 2,240 enrolled individuals, with 560 patients with psychosexual disorders and 1,680 subjects without psychosexual disorders (1:3) matched for age and index year, from the Longitudinal Health Insurance Database, retrieved from the National Health Insurance Research Database (NHIRD), between 2000-2015 in Taiwan. The multivariate Cox regression model was used to compare the risk of developing psychiatric disorders during the 15 years of follow-up. There were 98 in the cohort with psychosexual disorders (736.07 per 100,000 person-year) and 119 in the cohort without psychosexual disorders (736.07 per 100,000 person-year) that developed psychiatric disorders. The multivariate Cox regression model revealed that the adjusted hazard ratio (HR) was 9.848 (95% CI= 7.298 — 13.291, p < 0.001), after the adjustment of age, monthly income, urbanization level, geographic region, and comorbidities. Female patients with psychosexual disorders were associated with the risk of psychiatric disorders. This finding could be a reminder for clinicians about the mental health problems in the patients with psychosexual disorders.
The German Corona Consensus Dataset (GECCO): A standardized dataset for COVID-19 research in university medicine and beyond
Authors: Sass Julian , Bartschke Alexander , Lehne Moritz , Essenwanger Andrea , Rinaldi Eugenia , Rudolph Stefanie , Heitmann Kai U. , Vehreschild Jörg J. , von Kalle Christof , Thun Sylvia 
Published: December 2020

Abstract
Background: The current COVID-19 pandemic has led to a surge of research activity. While this research provides important insights, the multitude of studies results in an increasing fragmentation of information. To ensure comparability across projects and institutions, standard datasets are needed. Here, we introduce the “German Corona Consensus Dataset” (GECCO), a uniform dataset that uses international terminologies and health IT standards to improve interoperability of COVID-19 data, in particular for university medicine.Methods: Based on previous work (e.g., the ISARIC-WHO COVID-19 case report form) and in coordination with experts from university hospitals, professional associations and research initiatives, data elements relevant for COVID-19 research were collected, prioritized and consolidated into a compact core dataset. The dataset was mapped to international terminologies, and the Fast Healthcare Interoperability Resources (FHIR) standard was used to define interoperable, machine-readable data formats.Results: A core dataset consisting of 81 data elements with 281 response options was defined, including information about, for example, demography, medical history, symptoms, therapy, medications or laboratory values of COVID-19 patients. Data elements and response options were mapped to SNOMED CT, LOINC, UCUM, ICD-10-GM and ATC, and FHIR profiles for interoperable data exchange were defined.Conclusion: GECCO provides a compact, interoperable dataset that can help to make COVID-19 research data more comparable across studies and institutions. The dataset will be further refined in the future by adding domain-specific extension modules for more specialized use cases.
Insoluble proteins catch heterologous soluble proteins into inclusion bodies by intermolecular interaction of aggregating peptides.
Authors: Carratalá Jose Vicente , Cisneros Andrés , Hellman Elijah , Villaverde Antonio , Ferrer-Miralles Neus 
Published: December 2020

Abstract
Background: Protein aggregation is a biological event observed in expression systems in which the recombinant protein is produced under stressful conditions surpassing the homeostasis of the protein quality control system. In addition, protein aggregation is related to conformational diseases in animals as transmissible prion diseases, and non-transmissible neurodegenerative diseases including Alzheimer, Parkinson's disease, amyloidosis and multiple system atrophy among others. At the molecular level, the presence of aggregating-prone domains in protein molecules act as seeding igniters to induce the accumulation of protein molecules in protease-resistant clusters by intermolecular interactions.Results: In this work the aggregating-prone performance of a small peptide (L6K2) with additional antimicrobial activity was studied and the relevance of the accompanying scaffold protein to enhance the aggregating profile of the fusion protein has been elucidated. Furthermore, it was demonstrated that the fusion of L6K2 to highly soluble recombinant proteins directs the protein to inclusion bodies (IBs) in E. coli through stereospecific interactions in the presence of an insoluble protein displaying the same aggregating-prone peptide (APP). Conclusions: These data suggest that the molecular bases of protein aggregation are related not only to the presence of aggregation-prone stretches, but to the net balance of protein aggregation potential. and not only to the presence of aggregation-prone stretches. This is ultimately presented as a generic platform to generate hybrid protein aggregates in microbial cell factories for biopharmaceutical and biotechnological applications.
Contribution of Autologous Omentum Transposition to the Regeneration of Renal Injuries in the Rat Model
Authors: Bilgic Tayfun , Ince Umit , Narter Fehmi 
Published: December 2020

Abstract
Aim: After renal trauma, surgical treatment is vital, but sometimes there may be loss of function due to fibrosis. We aimed to evaluate the repair effect of transpositioned autologous omentum on injured renal tissue in a rat model.Methods: A total of 30 female Wistar Albino rats were included and they were randomly separated into a sham group and four study groups. Iatrogenic renal injuries were repaired using a surgical technique (primary repair 1 and 2 groups) or transpositioned autologous omentum (omentum repair 1 and 2 groups). In all groups, blood samples were taken preoperatively and on the 7th postoperative day in all groups and also on the 18th postoperative day in the control and two study groups. All rats were sacrificed on the 7th or 18th day postoperatively and their right kidneys were taken for histopathological evaluation. Results: There was a trend toward decrease in urea and creatinine levels in all the groups. There was no significant correlation between urea and creatinine levels and histological finding scores. The omentum repair group had significantly lower inflammation and granulation scores compared with the primary repair and sham groups. There was a significant and positive correlation between inflammation and granulation and fibrosis scores. There was a significant and negative correlation between healing completion score and either inflammation and granulation scores. There were also positive correlations between histological findings in the kidney specimen and surrounding tissues. Conclusion: The use of the autologous omentum tissue for repair of kidney injury had attenuation effects on inflammation and granulation compared with primary repair. These results imply that use of omentum tissue to facilitate healing of kidney injury may theoretically lead to a more effective healing process and reduced fibrosis and tissue and function loss. These potentially beneficial effects of autologous omentum tissue should be investigated in further well-designed experimental and clinical studies.
Effectiveness of a multidimensional intervention program in improving occupational musculoskeletal disorders among intensive care unit nurses: a cluster-controlled trial with follow-up at 3 and 6 months
Authors: Yang Shuai , Li Li , Wang Liqian , Zeng Jiaqi , Yan Bin , Li Yinglan 
Published: December 2020

Abstract
Background: Intensive care unit (ICU) nurses are at high risk for work-related musculoskeletal disorders (WRMDs). Data on occupational injuries indicate the significance of WRMDs among ICU nurses. Intervention programs have previously been developed to reduce WRMDs, but different intervention methods need to be adopted for different groups of people. This study aimed to evaluate the effectiveness of a multidimensional intervention program to prevent and reduce WRMDs in ICU nurses.Methods: This study was designed as a two-armed cluster-controlled trial with an intervention group and a control group. The clusters were independent hospital ICUs, and the participants consisted of registered nurses in China. By cluster random sampling, 89 nurses from two ICUs were assigned to the intervention group, and 101 nurses from two other ICUs were assigned to the control group. A multidimensional intervention program based on previous studies was designed. This program combined improving risk perception, health behavior training, and promoting a safe working environment. The multidimensional intervention program was implemented in the intervention group, whereas routine specialist training was implemented in the control group. Baseline and follow-up (3 and 6 months) data were collected using self-reported online questionnaires. The primary outcome was the report rate of WRMDs in the past 7 days. Secondary outcomes were risk perception, application of health behavior, and perception of a safe working environment. The data were statistically analyzed using SPSS 19.0.Results: A total of 190 nurses provided three recorded outcome measurements (intervention group, N = 89 (94.68%); control group, N = 101 (94.39%)). After 6 months, the intervention group experienced significant improvement relative to the control group in the report rate of WRMDs in the past 7 days (OR = 1.953, p = 0.037), risk perception (OR = 0.517, p < 0.001), application of health behavior (OR = 0.025, p < 0.001), and perception of a safe working environment (OR = 1.637, p = 0.024).Conclusion: The multidimensional intervention program was superior to routine specialist training in preventing the occurrence of WRMDs in ICU nurses. WRMD training should include multifaceted approaches and pay increased attention to specific department functions.
S100A8 and S100A9 in saliva, blood and gingival crevicular fluid for screening periodontitis: a cross-sectional study
Authors: Kim Hyun-Duck , Karna Sandeep , Shin YooJin , Vu Huong , Cho Hyun-Jae , Kim Sungtae 
Published: December 2020

Abstract
Background: Periodontitis is one of major oral diseases, which has no consensus on early screening tool. This study aimed to compare the association and screening ability of S100A8 and S100A9 in saliva, blood and gingival crevicular fluid (GCF) for periodontitis.Methods: We recruited 149 community Koreans, 50 healthy and 99 periodontitis. Using clinical attachment loss and a panoramic radiograph, stage II-IV of new classification of periodontitis proposed at 2018 was considered as periodontitis. Enzyme linked immunosorbent assay kit was used to quantify S100A8 and S100A9. T-test, analysis of covariance (ANCOVA), Mann-Whitney test and correlation analysis were applied to compare the relationship of S100A8 and S100A9 in saliva, blood, and GCF for periodontitis. Receiver operating characteristic curve was applied for screening ability.Results: Among S100A8 and S100A9 in saliva, blood and GCF, S100A8 in saliva was significantly higher in periodontitis participants than in healthy participants (p<0.05) and showed highest screening ability of 0.73 for periodontitis. However, S100A8 and S100A9 in GCF were significantly higher in healthy participants (p<0.05). Salivary S100A8 was positively correlated to blood S100A8 (r=0.21, p <0.05).Conclusion: Salivary S100A8 could be a potential diagnostic marker for periodontitis. Thus, S100A8 salivary kit will be useful for screening periodontitis.
Development and characterization of thirteen novel microsatellite markers for use in Greenland sharks (Somniosus microcephalus), with cross-amplification in Pacific sleeper sharks (Somniosus pacificus)
Authors: Swintek Meaghan A , Walter Ryan P 
Published: December 2020

Abstract
Objective: The objectives of this work are to isolate, develop, and characterize polymorphic microsatellite markers for use in Greenland sharks (Somniosus microcephalus). Results: Thirteen microsatellite loci were successfully amplified and yielded multi-locus genotypes for 36 S. microcephalus individuals from Grise Fjord (n = 16) and Svalbard (n = 20). Each locus yielded between 2 to 9 alleles and observed heterozygosity ranged from 0.11 to 0.70 when estimated across both sites. One locus and three loci deviated from HWE following Bonferroni correction, for individuals sampled from Grise Fjord and Svalbard, respectively. Cross-amplification was successful at every locus for five of the ten S. pacificus individuals.