bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bioinformatics analysis of the expression of inducible nitric oxide synthases (iNOS/NOS2) in human glioma and its correlation with patients’ prognoses
Authors: Zhang Liping , Wang Huanyu , Feng Mei , Zhang Xueqing 
Published: July 2020

Abstract

Objective To evaluate the expression of inducible nitric oxide synthases (iNOS/NOS2) in human glioma and its correlation with patients’ prognoses.

Methods IiNOS/NOS2 expression in tumor and corresponding normal tissues of glioma patients was analyzed using the TCGA database and the online analysis tool GEPIA. The mutation statuses of iNOS/NOS2 genes were also explored in the TCGA database using cBioPortal. Co-expressed genes relevant to iNOS/NOS2 were screened by LinkedOmics. Gene ontology (GO) and KEGG pathway enrichment for iNOS/NOS2 and co-expressed genes was performed using LinkedOmics. Overall survival (OS) and disease-free survival (DFS) outcomes between iNOS/NOS2 mRNA high and low expression groups were compared using a log-rank test. Twenty-two glioma patients who underwent operation were included in the present work. A real-time PCR assay was used to detect iNOS/NOS2 mRNA expression in tumor tissue and normal brain tissue.

Results There was no statistical difference in iNOS/NOS2 mRNA expression levelss between tumor and normal tissues of glioma. A real-time PCR assay indicated that iNOS/NOS2 mRNA expression in tumor tissue and normal brain tissues were not statistical difference (p>0.05). A mutation rate of 0.8% for the iNOS/NOS2 gene was found using 1044 glioma patients from two datasets. The mutation types include deep deletion (0.4%), truncating (0.2%) and missense (0.2%). The top positive and negative co-expressed gene with iNOS/NOS2 were COL25A1 (rpearson=0.4734, p<0.05) and ALCAM (rpearson=0.4734, p<0.05), respectively. For KEGG pathway analysis, iNOS/NOS2 was mainly enriched in calcium signaling pathway, Wnt signaling pathway, GnRH signaling pathway, HIF-1 signaling pathway and pathways in cancer. The overall survival (HR=2.0, p<0.05) and disease-free survival (HR=1.6, p<0.05) values were significantly different between iNOS/NOS2 high and low expression groups.

Conclusion OS and DFS were significantly decreased in high iNOS/NOS2 mRNA expression groups. iNOS/NOS2 can be used as a poor prognostic biomarker for glioma.
Direct Interconnection and Investment Incentives for Content Quality
Authors: Kim Soo Jin 
Published: July 2020

AbstractThis paper analyzes the effects of direct interconnection agreements in the Internet backbone on content quality investment for content providers (CPs). The model assumes that when the Internet service provider (ISP) has a vertical affiliation with one CP, the ISP directly interconnects the affiliated CP’s traffic to its network for free while collecting a direct interconnection fee from the unaffiliated CP. If the unaffiliated CP’s traffic is indirectly interconnected to the ISP’s network via a third party transit provider, its network quality is lower than that via a direct interconnection. For the CPs’ content quality investments, I find that the affiliated CP invests more in content when the rival indirectly interconnects, leading to a higher total level of content investment. Accordingly, there is a condition under which the ISP does not want to offer direct interconnection to the unaffiliated CP. However, consumers are not always worse off from this interconnection foreclosure. Thus, the regulation of a paid direct interconnection does not necessarily enhance welfare in terms of consumer surplus.
Genome-Wide Dynamic Evaluation of the UV-Induced DNA Damage Response
Authors: Silva Erica , Michaca Manuel , Munson Brenton , Bean Gordon J , Jaeger Philipp A , Licon Katherine , Winzeler Elizabeth A , Ideker Trey 
Published: July 2020

Abstract
Genetic screens in Saccharomyces cerevisiae have allowed for the identification of many genes as sensors or effectors of DNA damage, typically by comparing the fitness of genetic mutants in the presence or absence of DNA-damaging treatments. However, these static screens overlook the dynamic nature of DNA damage response pathways, missing time-dependent or transient effects. Here, we examine gene dependencies in the dynamic response to ultraviolet radiation-induced DNA damage by integrating ultra-high-density arrays of 6144 diploid gene deletion mutants with high-frequency time-lapse imaging. We identify 494 ultraviolet radiation response genes which, in addition to recovering molecular pathways and protein complexes previously annotated to DNA damage repair, include components of the CCR4-NOT complex, tRNA wobble modification, autophagy, and, most unexpectedly, 153 nuclear-encoded mitochondrial genes. Notably, mitochondria-deficient strains present time-dependent insensitivity to ultraviolet radiation, posing impaired mitochondrial function as a protective factor in the ultraviolet radiation response.
The Berriasian–Aptian of the Western Gydan Peninsula (West Siberia): Biofacies and Lithofacies Models
Authors: Vakulenko L.G. , Ershov S.V. , Nikolenko O.D. , Pestchevitskaya E.B. , Popov A.Yu. , Yan P.A. 
Published: July 2020

Abstract
—Well logs and core analyses of Berriasian–Aptian petroliferous sediments from the western Gydan Peninsula are used to model their biofacies and lithofacies and to reconstruct the respective sequence stratigraphy. The sedimentological and palynological data, along with logging results, provide constraints on the history of transgressive and regressive events and make a basis for paleogeographic reconstructions for the Lower Cretaceous deposition of reservoir sand beds.
Manifestation of Induction and Induced Polarization in the Case of Axial and Symmetrical Electrical Arrays
Authors: Ageenkov E.V. , Sitnikov A.A. , Pesterev I.Yu. , Popkov A.V. 
Published: July 2020

Abstract
—The paper considers the manifestation of electromagnetic (EM) signal over the conducting polarizable ground on the measuring lines located on the axis of the source and inside it (for the symmetric Schlumberger installation). The research is based on a numerical experiment. Calculations of the EM response from one-dimensional models were carried out. The polarizability is taken into account by the frequency-dependent resistivity, using the Cole-Cole model. We describe the results of a numerical experiment on calculation of the induction signal over a conductive polarizable medium on grounded 2- and 3-electrode measuring lines located in the axial and equatorial zones of the source. It is shown that the induced polarization and the polarization associated with galvanic and eddy current are manifested in different ways.
ВІД МІСТА-ПЕРЕХРЕСТЯ ДО МІСТА-ЖЕРТВИ: ЛЬВІВ У РОМАНІ ЮРІЯ ВИННИЧУКА «ТАНҐО СМЕРТІ»
Authors: Монолатій Іван 
Published: July 2020

У статті на основі аналізу особливостей роману Юрія Винничука «Танґо смерті» проаналізовано авторські наративні стратегії переосмислення на прикладі Львова сюжетів міської історії у 1930–1940-х роках, реконструкції колективної і персональних пам’ятей містян. Проаналізований письменницький текст є суттєвим прикладом «вписування» Львова у пам’яттєвий дискурс сучасної української літератури, а тому має два часові зрізи – довоєнний/воєнний (міжвоєнний період, роки Другої світової війни) і сучасний. Досліджувані події вивчаються як історія містян (зокрема взаємин дітей бійців Армії УНР, розстріляних 1921-го під Базаром), які є носіями різних, часто конфліктних національних ідентичностей, виклику щодо можливостей реконструкції пам’яті про «спільний» Львів в умовах військово-політичного конфлікту і радикального загострення міжнаціональних взаємин, а також як відновлення пам’яті про Янівський концентраційний табір. Стверджується, що уявну та справжню поліфонію взаємин у передвоєнному Львові (як і в інших містах Галичини), а також дитинство-юність героїв «історичної» частини роману Ю. Винничука руйнує вересень 1939 року. Події Другої світової війни, описані на прикладі Львова, є важливим компонентом міської пам’яті, а пережиті страждання героїв роману можна назвати одним із потужних детермінантних елементів національної ідентичності. У своєму романі Ю. Винничук вдається до отілеснення музики, інсценізуючи текст роману як «танґо», створюючи тим самим партитуру поліфонічності Львова з міста-перехрестя у місто-жертву з його мешканцями. На прикладі роману Ю. Винничука показано, що «львівська» пам’ять є маркером темпорального горизонту, зокрема комунікативна пам’ять передає історичний досвід міста у межах індивідуальних біографій протагоністів роману за допомогою живих спогадів, безпосереднього досвіду й усної оповіді. Запропоновано розглядати письменника як носія мнемонічного інформаційного ресурсу, того, хто накопичує пам’ять про трагічні сторінки міської історії і ретранслює до своїх сучасників. Аналіз роману Ю. Винничука «Танґо смерті» показує, що, по-перше, Львів є прикладом не так символічного, як цілком реального місця пам’яті, і, по-друге, підтвердженням сучасної тези А. Ассман про місце пам’яті, що «освячене присутністю мертвих».
Care Coordination Program for Children With Complex Chronic Conditions Discharged From a Rural Tertiary-Care Academic Medical Center
Authors: Parker Clayten L. , Wall Bennett , Tumin Dmitry , Stanley Rhonda , Warren Lana , Deal Kathy , Stroud Tara , Crickmore Kim , Ledoux Matthew 
Published: July 2020

OBJECTIVES:
Hospital discharge offers an opportunity to initiate coordination of follow-up care, preventing readmissions or emergency department (ED) recidivism. We evaluated how revisits and costs of care varied in a 12-month period between children in a care coordination program at our center (enrolled after hospital discharge with a tracheostomy or on a ventilator) and children with complex chronic condition discharges who were not enrolled.

METHODS:
Children ages 1 to 17 years were retrospectively included if they had a hospital discharge in 2017 with an International Classification of Diseases, 10th Revision code meeting complex chronic condition criteria or if they were in active follow-up with the care coordination program. Revisits and total costs of care were compared over 2018 for included patients.

RESULTS:
Seventy patients in the program were compared with 56 patients in the control group. On bivariate analysis, the median combined number of hospitalizations and ED visits in 2018 was lower among program participants (0 vs 1; P = .033), and program participation was associated with lower median total costs of care in 2018 ($700 vs $3200; P = .024). On multivariable analysis, care coordination program participation was associated with 59% fewer hospitalizations in 2018 (incidence rate ratio: 0.41; 95% confidence interval: 0.23 to 0.75; P = .004) but was not significantly associated with reduced ED visits or costs.

CONCLUSIONS:
The care coordination program is a robust service spanning the continuum of patient care. We found program participation to be associated with reduced rehospitalization, which is an important driver of costs for children with medical complexity.
Discharge Communication Practices for Children With Medical Complexity: A Retrospective Chart Review
Authors: Rush Margaret , Herrera Nicole , Melwani Anjna 
Published: July 2020

OBJECTIVES:
Children with medical complexity (CMC) have an increased risk of adverse events after hospital discharge. Authors of previous studies have evaluated discharge communication practices with primary care providers (PCPs) in adults and general pediatric patients. There is a lack of evidence surrounding hospitalist communication practices at discharge for CMC. In this study, we explore hospitalist-to-PCP communication for CMC at hospital discharge.

METHODS:
A retrospective chart review was performed at a single tertiary care children’s hospital. The population included patients with ≥1 complex chronic condition who were discharged from the pediatric hospitalist team. The presence, type, and quality of discharge communication were collected. A descriptive analysis in which we used χ2, t test, Wilcoxon rank testing, and odds ratios was conducted to identify differences in communication practices in CMC.

RESULTS:
We identified 368 eligible patients and reviewed their electronic medical records. Discharge communication was documented for 59% of patient encounters. Communication was less likely to occur for patients with technology dependence (P = .01), older patients (P = .02), and those who were admitted to a teaching service (P = .04). The quality of discharge summaries did not change for patients with technology dependence compared with patients without technology dependence.

CONCLUSIONS:
Communication with the PCP at discharge was less likely to be documented in children with technology dependence. Hospitalists may encounter barriers in completion of appropriate and timely discharge communication with PCPs for CMC. Consistent handoff processes could be used to improve care for our patients with enhanced coordination needs.
Longitudinal Prevalence of Tracheostomized Children in Minnesota
Authors: Muesing Chad , Schimelpfenig Brianna , Hustvet Derek , Maynard Roy , Christensen Eric W. 
Published: July 2020

OBJECTIVES:
To identify the annual prevalence of tracheostomized children over an 11-year span in Minnesota. Secondary objectives included decannulation and mortality rates.

METHODS:
Retrospective review from 2008 to 2018 of a pediatric home care company database that provides home care services for ≥95% of tracheostomized children in Minnesota. The study group was divided into 2 cohorts: tracheostomized children that never required invasive home mechanical ventilation (iHMV) and tracheostomized children that required iHMV. Outcome measures included prevalence rates of tracheostomized children with or without iHMV, primary diagnoses, decannulation, and death rates.

RESULTS:
Prevalence rates for tracheostomized children ≤16 years of age per 100 000 population increased 39% from 12.3 in 2008 to 17.2 in 2018. This upward trend was primarily associated with an increase in tracheostomized children receiving iHMV. This prevalence trend was inversely correlated with age. There was no significant change in the decannulation rate. The annual all-cause death rate declined over the study period and was primarily associated with decreased mortality in children receiving iHMV. If national prevalence rates in 2018 were comparable to Minnesota, the results suggest there were 2839 pediatric home care patients with tracheostomies not requiring iHMV and 9024 children receiving iHMV.

CONCLUSIONS:
The population of tracheostomized children in Minnesota is expanding at a disproportionate rate relative to the population of children at large. This increase is predominantly associated with an increase in iHMV. Although the annual all-cause death rate has declined over the past decade, there was no change in the decannulation rate.
Dynamics of indices of metabolism in muscle tissue, liver and gonads of Abramis brama in different periods of the annual cycle
Authors: Payuta A. A. , Flerova E. A. 
Published: July 2020

The aim of this research was to study the dynamics of metabolism in the body of bream Abramis brama (L.) at different periods of the annual cycle: pre-spawning, spawning, post-spawning and feeding. The object of the study was 55 male and 53 female bream aged 9–13 years, to reduce the effect of age on the studied parameters. Individuals were measured, weighed, gender was determined, gonad maturity stage, age; the condition factor and organ indices were calculated. The content of moisture, dry matter, lipids, proteins, ash and nitrogen-free extracts (NFE) in the muscles, liver and gonads of male and female bream have been analyzed. The lowest coefficients of condition factor were found in bream during the autumn feeding season, the highest in males during spawning, in females before and after spawning. In the muscle tissue of male and female bream the content of metabolic products in the feeding period and before spawning differed slightly. In the spawning period, the muscles of males exceeded the muscle tissue of females in lipid content by more than two times. In the annual cycle of the bream, the ovaries contain more protein than the testes. The bream of different sex revealed differences in the intensity of metabolic processes during the maturation of gonads: in males, energy costs are more pronounced after wintering before spawning, while in females, during spawning. The main energy reserve in the form of lipids in the annual cycle changes more significantly in the body of male bream than in females. Ovarian maturation requires more metabolite reserves than maturation of the testes, however, males have increased expenditure associated with reproductive behaviour during spawning. Therefore, the total energy contribution to reproduction may be the same for female and for male bream. Bream’s liver and muscle tissue can serve as a source of energy during food shortages and during spawning. In the annual cycle, the condition factor of bream increases due to an increase in the moisture content in the muscles.
Are Heteroptera communities able to be bioindicators of urban environments?
Authors: Kunakh O. M. , Fedyay I. O. 
Published: July 2020

The Heteroptera is a group of animals associated with the vegetation cover. The conducted analysis indicates that groups of heteropterans in the urban environment have a pattern of reaction to the environmental factors, determined using the phytoindication method. In the study, we considered the following hypotheses: 1) phytoindicational assessments of ecological factors may explain the patterns of variation of the groups of heteropterans; 2) among Heteroptera species, comparatively homogenous ecological groups could be distinguished which are characterized by similar character of response to the effect of certain environmental factors; 3) these groups could be used for bioindication of the conditions of environment in urban ecosystems. Stationary collection of heteropterans was performed during three years from May to October of 2017–2019 on six plots in Kharkiv. The article describes factors which affect the structure of groups of Heteroptera within the ecosystem of the large city and assess the bioindication possibilities. The data presented in the article, as well as the conclusions drawn, are to a large extent associated with stenotopic species, most of which could be used as bioindicators of the condition of one or another biocenosis. According to the results of a taxonomical survey in the territory of Kharkiv, 180 species of Heteroptera were found, belonging to 120 genera and 17 families. The highest species diversity was seen for the family Miridae, accounting for 50 species (27.0% of the total number of counted species). Fewer species were identified as the representatives of families Lygaeidae – 46 species (24.9%) and Pentatomidae – 23 (12.4%). Family Rhopalidae was represented by 11 species (5.9%). Nabidae and Tingidae – 10 species each (5.4%). Families Coreidae – 8 (4.3%), Cydnidae and Scutelleridae – 4 species each (2.2%), Anthocoridae – 3 (1.6%). The families Berytidae, Piesmatidae, Pyrrhocoridae and Reduviidae were represented by only 2 species each (1.1%). Families Acanthosomatidae, Alydidae and Aradidae were represented by 1 species each, in total accounting for 1.5%. The reasonably high level of species and ecological diversities of Heteroptera in the territory of the city allows them to be used in bioindication studies. We determined comparatively homogenous ecological groups of heteropterans which have a similar pattern of response to the impact of certain environmental factors. The study demonstrates that phytoindicatory assessments of the ecological factors can explain the patterns of variation in groups of heteropterans, We determined the factors which have effects on the structure of the group of heteropterans within the metropolitan ecosystem. The level of their effect on groups of heteropterans within the city is different. The most influential were light and humidity. Comparison of potential and realized projections of ecological space allows us, to a certain extent, to generate hypotheses about the orientations of transformation of the group heteropterans.
A comparative study of efficacy, safety and compliance of oral iron versus intravenous iron sucrose in treatment of iron deficiency anaemia of pregnancy
Authors: Thobbi Vidya A. , Bijapur Zeba Naz M. 
Published: July 2020

Background: Iron deficiency anemia is the most common form of anemia and nutritional disorder worldwide. Oral iron therapy and blood transfusion has many drawbacks like noncompliance and risk of transmittable infections and transfusion reaction. The modern alternative therapy is treatment with intravenous iron. Present study compares the efficacy, safety and tolerability between intravenous iron sucrose and oral iron in iron deficiency anemia during 20-36 weeks of pregnancy.Methods: It was a randomized controlled study between December 2017 to September 2019. 200 patients attending antenatal OPD in Al Ameen Medical College, with haemoglobin levels between 7-9.9 gm/dl and serum ferritin of <15 ng/ml were enrolled. In intravenous group, 200 mg iron sucrose in 100ml normal saline was infused alternate day till the required dose was met. The oral group received 200 mg of oral iron ascorbate along with folic acid 1.5 mg per day for 6 weeks. Treatment efficacy was assessed by Hb and serum ferritin after 3 and 6 weeks.Results: Out of 200 patients, an increase in Hb was observed in both groups, rising from 9.7 g/dl to 10.3 g/dl and 10.9 g/dl after 3 weeks and 6 weeks respectively in oral group and from 8.6 g/dl to 9.8 g/dl and 10.8 g/dl after 3 weeks and 6 weeks respectively in intravenous group. Similar results were seen in ferritin levels. Rise in Hb and ferritin levels were greater in intravenous group than in oral group.Conclusions: Intravenous iron sucrose appears to be a better treatment option in comparison with oral iron, without serious side effects, better compliance and improved efficacy in correction of anaemia of pregnancy.
Radiographic imaging of mastoid in chronic otitis media: need or tradition?
Authors: Beig Sabeeh , Khalid Saifullah , Sharma Satish Chandra 
Published: July 2020

<p class="abstract"><strong>Background:</strong> Computed tomography is the imaging of choice in chronic otitis media (COM) but it is neither available at every centre nor is affordable to masses of economically weaker countries. In this situation where only X-ray facility is available should plain radiographs of mastoid be done ?. If yes then what is the analytical evidence? This study, was conducted to find the utility of plain radiographs of mastoid by comparing radiological findings vis-a-vis operative findings.</p><p class="abstract"><strong>Methods:</strong> Pre-operative radiographs of mastoids (Schuller’s view (s/v)) were taken and the radiological findings were statistically analysed with the operative findings. </p><p class="abstract"><strong>Results:</strong> Plain radiograph of mastoid (s/v) predicted some of the surgical landmarks of mastoid surgery viz tegmen and sinus plates with a fair degree of accuracy. The positive predictive value (PPV) for radiolucent shadow (assumed to indicate bone destruction and thus cholesteatoma) was also high but at the same time, a low negative predictive value and a Cohen’s kappa test showing only a fair agreement underscores the point that absence of a radiolucent shadow does not rule out the presence of cholesteatoma.</p><p class="abstract"><strong>Conclusions:</strong> Radiographs of mastoid are helpful in providing a prior knowledge of the surgical landmarks in mastoid surgery. Hence with this information, if a surgeon finds himself more at ease in operating a patient then this imaging should be done when CT scan facility is unavailable. However, citing the limited information on other aspects of the disease, its use as a ‘routine’ investigation in chronic otitis media is discouraged.</p><p class="abstract"> </p>