bluuebunny/crossref_metadata_2025_split huggingface dataset:
The impact of sex and disease classification on patient-reported outcome measures in axial spondyloarthritis: a descriptive prospective cross-sectional study
Authors: Andreasen Rikke A. , Kristensen Lars E. , Egstrup Kenneth , Baraliakos Xenofon , Strand Vibeke , Horn Hans Christian , Hansen Inger M. J. , Christensen Robin , Ellingsen Torkell 
Published: October 2019

Abstract

Background
The aim of this study was to explore the impact of sex and disease classification on outcomes in axial spondyloarthritis (axSpA) patients, including both radiographic (r-) axSpA and non-radiographic (nr-) axSpA, in males and females, respectively.

Methods
AxSpA patients were consecutively recruited from two rheumatology outpatient university clinics. We explored how sex and axSpA disease classification affected patient-reported outcome measures (PROMs). General linear models were used to investigate if there was an association between the continuous variables and each of the main effects of interest (sex and axSpA classification), as well as the possible interaction between them. Categorical outcome measures were analyzed with the use of logistic regression with the same fixed effects. We analyzed the relationship between tender point count (TPC) and the Bath Ankylosing Spondylitis Disease Activity Index (BASDAI). The prevalence of extra-articular manifestations (EAMs) and the Charlson Comorbidity Index (CCI) were determined.

Results
According to the protocol, a total of 100 outpatients with axSpA were enrolled (r-axSpA males 30, r-axSpA females 10, nr-axSpA males 25, nr-axSpA females 35). The BASDAI scores appeared higher among nr-axSpA females (median [Q1; Q3], 47 [21; 60]) compared with the combined median for the 3 other subgroups 25 [12; 25]. Female sex was associated with a higher number of tender point count (TPC, P < 0.001). TPC and BASDAI were correlated for female nr-axSpA patients (r = 0.44, P = 0.008) and male nr-axSpA patients (r = 0.56, P = 0.003). Being classified as nr-axSpA was associated with a lower SF-36 Mental Component Summary (median for the 4 subgroups: nr-axSpa females 46.7, nr-axSpA males 52.3 vs. r-axSpA males 56.9 and r-axSpA females 50.4). EAMs were frequent (up to 50%). The CCI was low in all 4 subgroups, and no difference in the CCI between the subgroups was observed (P = 0.14). However, male sex had a significant impact on the CCI (P = 0.03).

Conclusions
In summary, patients with r-axSpA, regardless of sex, appeared less affected on most PROMs compared with nr-axSpA patients. However, female sex was associated with a higher number of TPC. TPC could possibly confound disease activity outcomes such as BASDAI, and one can consider different thresholds for defining high disease activity depending on the patient’s sex.

Trial registration
The trial is registered and approved by the Region of Southern Denmark’s Ethics Committee (S-20150219). Registered 19 February 2015.
Effects of vitamin D supplementation on advanced glycation end products signaling pathway in T2DM patients: a randomized, placebo-controlled, double blind clinical trial
Authors: Omidian Mahsa , Djalali Mahmoud , Javanbakht Mohammad Hassan , Eshraghian Mohammad Reza , Abshirini Maryam , Omidian Parisa , Alvandi Ehsan , Mahmoudi Maryam 
Published: October 2019

Abstract
Background
Several researches have recommended vitamin D possible health benefits on diabetic complications development, but a few number of studies have been accomplished on the molecular and cellular mechanisms. Certain cellular pathways modification and also some transcription factors activation may protect cells from hyperglycemia condition induced damages. This study purpose was to determine the vitamin D supplementation effect on some key factors [advanced glycation end products (AGEs) signaling pathway] that were involved in the diabetic complications occurrence and progression for type-2 diabetes participants.

Methodology
48 type-2 diabetic patients (T2DM) randomly divided into two groups (n = 24 per group), receiving: 100-µg vitamin D or placebo for 3 months. At this study beginning and the end, the receptor expression for advanced glycation end products (RAGE) and glyoxalase I (GLO1) enzyme from peripheral blood mononuclear cells (PBMCs) and AGEs and tumor necrosis factor-α (TNF-α) serum levels were measured by the use of real-time PCR and ELISA methods, respectively.

Results
This study results demonstrated that vitamin D supplementation could down-regulate RAGE mRNA [fold change = 0.72 in vitamin D vs. 0.95 in placebo) P = 0.001)]. In addition, no significant changes were observed for GLO1 enzyme expression (P = 0.06). This study results also indicated that vitamin D serum level significantly increased in vitamin D group (P < 0.001). Moreover, AGES and TNF-α serum levels significantly reduced in vitamin D group, but they were remained unchanged in the placebo group.

Conclusion
In conclusion, vascular complications are more frequent in diabetic patients, and vitamin D treatment may prevent or delay the complications onset in these patients by AGEs serum level and RAGE gene expression reducing.
Trial registration NCT03008057. Registered December 2016
Risky sexual behavior practice and associated factors among secondary and preparatory school students of Aksum town, northern Ethiopia, 2018
Authors: Girmay Alem , Mariye Teklewoini 
Published: October 2019

Abstract

Objective
As adolescence is a stage in which human beings face once throughout a lifetime and it is the experimental period for this reason risky sexual behavior is common among young people, and it has several sexual and reproductive health consequences. But it doesn’t get enough attention the status of risky sexual behavior practice and factors’ contributing to it, so this study aimed to identify the prevalence of risky sexual behavior and factors associated with it.

Results
From the total respondents 115 (23.7%) had history of sexual intercourse and the mean age for sexual initiation is 15.48 ± 1.99 year. From whom 110 (98.2%) had sexual contact below the age of 18 and only 68 (60.8%) initiate first sex by their own willing. Overall 97 (19.6%) had practiced risky sexual behaviors. Students not facing peer pressure were 0.36 times less likely to develop risk sexual behavior (AOR = 0.357, 95% CI 0.172, 0.744).
Burden of Shigella spp and Vibrio spp, and their antibiotic sensitivity pattern in the patients with acute gastroenteritis in tertiary care hospital in Nepal
Authors: Sah Shiv Kumar , Basnet Shreejana , Shrestha Sushma , Ghale Kusum , Tamang Sabita , Mandal Dipendra Kumar , Pun Sher Bahadur 
Published: October 2019

AbstractObjectivesThe present study aims to investigate the etiology, clinical profile and resistance pattern of the isolated pathogens in Nepalese adults with acute gastroenteritis. This cross-sectional study was conducted at Sukraraj Tropical and Infectious Disease Hospital, from April 2016 to Sep 2017. Subjects’ ages 14 or above, presenting with gastroenteritis with positive stool culture were enrolled for analysis.ResultsOf total 153 patients, 47.72% subjects confirmed the presence of bacterial infection.Vibrio cholerae spp andShigellaspp were detected in 36.6% and 23.28% respectively. The most common resistance amongVibrio cholerae was to nitrofurantoin (92.8%), cotrimoxazole (92.8%) and nalidixic acid (92.8%). Among 17 isolates ofShigellaspp, the most frequent drug resistant was observed in ampicillin (64.7%), nalidixic acid (58.8%), ceftriaxone (47%). Chloramphenicol (94.1%), tetracycline (88.2%), and cotrimoxazole (82.3%) were found to be the most sensitive towards this pathogen. High rate of diarrhea due to bacterial infection, especiallyShigellaspp andVibriospp and their high rate of drug resistance emphasize an urgent need of designing a surveillance system for antimicrobial resistance in Nepalese setting.
Prevalence and antimicrobial susceptibility profile of Salmonella and Shigella among food handlers working in food establishment at Hawassa city, Southern Ethiopia
Authors: Awol Neja , Nigusse Demiss , Ali Musa 
Published: October 2019

AbstractObjectiveThe aim of this study was to determine the prevalence ofSalmonellaandShigella, antibiotic susceptibility profile and associated factors among food handlers working in food establishment from June to December 2018 at Hawassa city, Southern Ethiopia.ResultsOut of the 236 food handlers screened for stool culture, 5 (2.12%) were positive forSalmonellaspecies and all of them were negative forShigellaspecies. AllSalmonellaspecies isolated were susceptible to ciprofloxacin and ceftriaxone but among the five isolatedSalmonellaspecies, 4 (80%), 3 (60%), 2 (40%), 2 (40%), and 2 (40%) were resistant to tetracycline, ampicillin, amoxicillin–clavulanic acid, cotrimoxazole, and chloramphenicol respectively. Only food handlers hand washing the habit after toilet had a significant association with the prevalence ofSalmonellaspecies (P = 0.03).
Hypermethylation of mismatch repair gene hMSH2 associates with platinum-resistant disease in epithelial ovarian cancer
Authors: Tian Hua , Yan Li , Xiao-fei Li , Hai-yan Sun , Juan Chen , Shan Kang 
Published: October 2019

AbstractPurposeOne major reason of the high mortality of epithelial ovarian cancer (EOC) is due to platinum-based chemotherapy resistance. Aberrant DNA methylation may be a potential mechanism underlying the development of platinum resistance in EOC. The purpose of this study is to discover potential aberrant DNA methylation that contributes to drug resistance.MethodsBy initially screening of 16 platinum-sensitive/resistant samples from EOC patients with reduced representation bisulfite sequencing (RRBS), the upstream region of thehMSH2gene was discovered hypermethylated in the platinum-resistant group. The effect ofhMSH2methylation on the cellular response to cisplatin was explored by demethylation and knockdown assays in ovarian cancer cell line A2780. Matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry was employed to examine the methylation levels ofhMSH2upstream region in additional 40 EOC patient samples. RT-qPCR and IHC assay was used to detect thehMSH2mRNA and protein expression in extended 150 patients.ResultsRRBS assay discovered an upstream region from − 1193 to − 1125 ofhMSH2was significant hypermethylated in resistant EOC patients (P = 1.06 × 10−14). In vitro analysis demonstrated that global demethylation increased cisplatin sensitivity along with a higher expression of the hMSH2 mRNA and protein. KnockdownhMSH2reduced the cell sensitivity to cisplatin. MALDI-TOF mass spectrometry assay validated the strong association of hypermethylation ofhMSH2upstream region with platinum resistance. Spearman’s correlation analysis revealed a significantly negative connection between methylation level ofhMSH2upstream region and its expression. The Kaplan-Meier analyses showed the high methylation ofhMSH2promoter region, and its low expressions are associated with worse survival. In multivariable models,hMSH2low expression was an independent factor predicting poor outcome (P = 0.03, HR = 1.91, 95%CI = 1.85–2.31).ConclusionThe hypermethylation ofhMSH2upstream region is associated with platinum resistant in EOC, and low expression ofhMSH2may be an index for the poor prognosis.
Neurochemical sex differences in adult ADHD patients: an MRS study
Authors: Endres Dominique , Tebartz van Elst Ludger , Maier Simon J. , Feige Bernd , Goll Peter , Meyer Simon A. , Matthies Swantje , Domschke Katharina , Lange Thomas , Sobanski Esther , Philipsen Alexandra , Nickel Kathrin , Perlov Evgeniy 
Published: October 2019

AbstractObjectiveAttention-deficit/hyperactivity disorder (ADHD) is a common neurodevelopmental disorder. Relevant sex differences in symptomatology are discussed. This study compared brain neurometabolism in the anterior cingulate cortex (ACC) and left cerebellar hemisphere in age- and IQ-matched adult male (mADHD) and female (fADHD) ADHD patients.MethodsWe studied 48 (ACC) and 42 (cerebellum) male/female pairs of stimulant-free patients with adult ADHD. Single voxel magnetic resonance spectroscopy (MRS) was used to investigate creatine (Cre), total choline (t-Cho), glutamate + glutamine (Glx), N-acetylaspartate, and myo-inositol. The mADHD and fADHD groups were compared using robust linear regression. The level of significance was corrected for multiple tests using the Benjamini-Hochberg approach.ResultsFor the ACC, the signals of Cre (p = 0.008) and t-Cho (p = 0.004) showed significant effects of the age covariate as well as an interaction of sex and age (Cre:p = 0.033; t-Cho:p = 0.040). For the Glx signal, an interaction of sex and age could also be observed (p = 0.033). For cerebellar neurometabolites, the signals of t-Cho (p = 0.049) and Glx (p = 0.049) showed significant effects of the factor sex.ConclusionThis is the largest study yet to analyze sex differences in brain neurochemistry in adult patients with ADHD. Different age-dependent t-Cho signals in the ACC might be associated with delayed myelinization in mADHD. Further MRS studies in adult ADHD, accounting for possible sex effects, are warranted to validate the present findings.
Preclinical development of HQP1351, a multikinase inhibitor targeting a broad spectrum of mutant KIT kinases, for the treatment of imatinib-resistant gastrointestinal stromal tumors
Authors: Liu Xuechao , Wang Guangfeng , Yan Xianglei , Qiu Haibo , Min Ping , Wu Miaoyi , Tang Chunyang , Zhang Fei , Tang Qiuqiong , Zhu Saijie , Qiu Miaozhen , Zhuang Wei , Fang Douglas D. , Zhou Zhiwei , Yang Dajun , Zhai Yifan 
Published: October 2019

Abstract

Background
Imatinib shows limited efficacy in patients with gastrointestinal stromal tumors (GISTs) carrying secondary KIT mutations. HQP1351, an orally bioavailable multikinase BCR-ABL inhibitor, is currently in clinical trials for the treatment of T315I mutant chronic myelogenous leukemia (CML), but the potential application in imatinib-resistant GISTs carrying secondary KIT mutations has not been explored.

Methods
The binding activities of HQP1351 with native or mutant KIT were first analyzed. Imatinib-sensitive GIST T1 and imatinib-resistant GIST 430 cells were employed to test the in vitro antiproliferative activity. Colony formation assay, cell migration assay and cell invasion assay were performed to evaluate the clonogenic, migration and invasion ability respectively. Flow cytometry and western blot analysis were used to detect cell apoptosis, cell cycle and signaling pathway. In vivo antitumor activity was evaluated in mouse xenograft models derived from GIST cell lines.

Results
HQP1351 potently inhibited both wild-type and mutant KIT kinases. In both imatinib-resistant and sensitive GIST cell lines, HQP1351 exhibited more potent or equivalent antiproliferative activity compared with ponatinib, a third generation BCR-ABL and KIT inhibitor. HQP1351 led to more profound inhibition of cell colony formation, cell migration and invasion, cell cycle arrest and cell apoptosis than ponatinib. Furthermore, HQP1351 also inhibited p-KIT, p-AKT, p-ERK1/2, and p-STAT3 to a higher extent than ponatinib. Finally, in xenograft tumor models derived from imatinib-resistant GIST cancer cell lines, HQP1351 exhibited antitumor activity superior to ponatinib.

Conclusions
Collectively, our in vitro and in vivo results suggest that the therapeutic application of HQP1351 in imatinib-resistant GIST patients deserves further investigation in clinical trials.
Coefficient bounds for certain subclasses of starlike functions
Authors: Cho Nak Eun , Kumar Virendra , Kwon Oh Sang , Sim Young Jae 
Published: October 2019

Abstract
The conjecture proposed by Raina and Sokòł [Hacet. J. Math. Stat. 44(6):1427–1433 (2015)] for a sharp upper bound on the fourth coefficient has been settled in this manuscript. An example is constructed to show that their conjectures for the bound on the fifth coefficient and the bound related to the second Hankel determinant are false. However, the correct bound for the latter is stated and proved. Further, a sharp bound on the initial coefficients for normalized analytic function f such that $zf'(z)/f(z)\prec \sqrt{1+\lambda z}$
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Positive solutions to second-order singular nonlocal problems: existence and sharp conditions
Authors: Ma Shiqi , Zhang Xuemei 
Published: October 2019

AbstractIn this paper we consider sharp conditions onωandffor the existence of$C^{1}[0,1]$C1[0,1]positive solutions to a second-order singular nonlocal problem$u''(t)+\omega (t)f(t,u(t))=0$u″(t)+ω(t)f(t,u(t))=0,$u(0)=u(1)=\int _{0} ^{1}g(t)u(t)\,dt$u(0)=u(1)=∫01g(t)u(t)dt; it turns out that this case is more difficult to handle than two point boundary value problems and needs some new ingredients in the arguments. On the technical level, we adopt the topological degree method.
Dynein activating adaptor BICD2 controls radial migration of upper-layer cortical neurons in vivo
Authors: Will Lena , Portegies Sybren , van Schelt Jasper , van Luyk Merel , Jaarsma Dick , Hoogenraad Casper C. 
Published: October 2019

Abstract
For the proper organization of the six-layered mammalian neocortex it is required that neurons migrate radially from their place of birth towards their designated destination. The molecular machinery underlying this neuronal migration is still poorly understood. The dynein-adaptor protein BICD2 is associated with a spectrum of human neurological diseases, including malformations of cortical development. Previous studies have shown that knockdown of BICD2 interferes with interkinetic nuclear migration in radial glial progenitor cells, and that Bicd2-deficient mice display an altered laminar organization of the cerebellum and the neocortex. However, the precise in vivo role of BICD2 in neocortical development remains unclear. By comparing cell-type specific conditional Bicd2 knock-out mice, we found that radial migration in the cortex predominantly depends on BICD2 function in post-mitotic neurons. Neuron-specific Bicd2 cKO mice showed severely impaired radial migration of late-born upper-layer neurons. BICD2 depletion in cortical neurons interfered with proper Golgi organization, and neuronal maturation and survival of cortical plate neurons. Single-neuron labeling revealed a specific role of BICD2 in bipolar locomotion. Rescue experiments with wildtype and disease-related mutant BICD2 constructs revealed that a point-mutation in the RAB6/RANBP2-binding-domain, associated with cortical malformation in patients, fails to restore proper cortical neuron migration. Together, these findings demonstrate a novel, cell-intrinsic role of BICD2 in cortical neuron migration in vivo and provide new insights into BICD2-dependent dynein-mediated functions during cortical development.
Study of a periodic spectral fluctuation existing in a fibered optical feedback cavity-enhanced absorption spectroscopy (OF-CEAS)
Authors: Luo Zhifu , Tan Zhongqi , Long Xingwu 
Published: October 2019

Abstract
A special periodic spectral fluctuation is observed during the study of a fibered high sensitivity optical feedback cavity-enhanced absorption spectroscopy (OF-CEAS) for the measurement of trace gas. This spectral fluctuation is different from some phenomenon observed in former OF-CEAS which contain a resonant cavity with V-shaped configuration, such as the etalon effect and the spectral ripple effect. To reveal why this phenomenon happens and how it works, a series of hypothesis are proposed and tested, and the results show that the multi-beam interference of resonance light at the input mirror of the resonant cavity is the main reason for this phenomenon. Based on the multi-beam interference theory, a mathematical modeling of this phenomenon is built, and the theoretical analyses agree well with the experimental results. Some methods to eliminate this phenomenon are proposed and implemented, and the 1σ noise equivalent absorption coefficient of 7.6 × 10− 10 cm− 1 Hz-1/2 is attained with this robust and compact OF-CEAS system.
Fully automated convolutional neural network-based affine algorithm improves liver registration and lesion co-localization on hepatobiliary phase T1-weighted MR images
Authors: Hasenstab Kyle A. , Cunha Guilherme Moura , Higaki Atsushi , Ichikawa Shintaro , Wang Kang , Delgado Timo , Brunsing Ryan L. , Schlein Alexandra , Bittencourt Leornado Kayat , Schwartzman Armin , Fowler Katie J. , Hsiao Albert , Sirlin Claude B. 
Published: October 2019

Abstract

Background
Liver alignment between series/exams is challenged by dynamic morphology or variability in patient positioning or motion. Image registration can improve image interpretation and lesion co-localization. We assessed the performance of a convolutional neural network algorithm to register cross-sectional liver imaging series and compared its performance to manual image registration.

Methods
Three hundred fourteen patients, including internal and external datasets, who underwent gadoxetate disodium-enhanced magnetic resonance imaging for clinical care from 2011 to 2018, were retrospectively selected. Automated registration was applied to all 2,663 within-patient series pairs derived from these datasets. Additionally, 100 within-patient series pairs from the internal dataset were independently manually registered by expert readers. Liver overlap, image correlation, and intra-observation distances for manual versus automated registrations were compared using paired t tests. Influence of patient demographics, imaging characteristics, and liver uptake function was evaluated using univariate and multivariate mixed models.

Results
Compared to the manual, automated registration produced significantly lower intra-observation distance (p < 0.001) and higher liver overlap and image correlation (p < 0.001). Intra-exam automated registration achieved 0.88 mean liver overlap and 0.44 mean image correlation for the internal dataset and 0.91 and 0.41, respectively, for the external dataset. For inter-exam registration, mean overlap was 0.81 and image correlation 0.41. Older age, female sex, greater inter-series time interval, differing uptake, and greater voxel size differences independently reduced automated registration performance (p ≤ 0.020).

Conclusion
A fully automated algorithm accurately registered the liver within and between examinations, yielding better liver and focal observation co-localization compared to manual registration.
Gibt es einen wissenschaftlichen Konsens zur Wirtschaftlichkeit nachhaltiger Immobilien?
Authors: Fuerst Franz , Dalton Ben 
Published: October 2019

ZusammenfassungEs besteht ein klarer wissenschaftlicher und gesellschaftlicher Konsens über die Schlüsselrolle, die Gebäuden und der gebauten Umwelt bei der Reduzierung von Treibhausgasemissionen und Ressourcenverbrauch zukommt. Unklar ist jedoch, inwieweit Umweltziele und ökonomische Rentabilität im Immobilienbereich miteinander einhergehen. Dieser Artikel analysiert 42 internationale Studien zur Rentabilitaet von Immobilien mit Nachhaltigkeitszertifikaten anhand einer Metaanalyse und kommt zu dem Schluss, dass diese Gebäude zumindest ein moderater Preis- und Mietpraemium erzielen. Obwohl es Hinweise darauf gibt, dass Studien, die positive Ergebnisse berichten, mit höherer Wahrscheinlichkeit veröffentlicht wurden, liefern diese Studien dennoch einen Anhhaltspunkt dafür, dass nachhaltiges Bauen und Sanieren vom Markt als wirtschaftlich sinnvoll erkannt und entsprechend bepreist wird. Dabei ist allerdings zu bedenken, dass der wirtschaftliche Erfolg umweltgerechter Immobilieninvestments unter anderem auch davon abhängt, inwieweit Marktmechanismen etabliert werden können, die ein effiziente Verteilung von Risiko und Rendite ermöglichen, wie zum Beispiel grüne Mietverträge oder Energiespar-Contracting.