bluuebunny/crossref_metadata_2025_split huggingface dataset:
Scientometric trends for coronaviruses and other emerging viral infections
Authors: Kagan Dima , Moran-Gilad Jacob , Fire Michael 
Published: August 2020

Abstract

Background
COVID-19 is the most rapidly expanding coronavirus outbreak in the past 2 decades. To provide a swift response to a novel outbreak, prior knowledge from similar outbreaks is essential.

Results
Here, we study the volume of research conducted on previous coronavirus outbreaks, specifically SARS and MERS, relative to other infectious diseases by analyzing &gt;35 million articles from the past 20 years. Our results demonstrate that previous coronavirus outbreaks have been understudied compared with other viruses. We also show that the research volume of emerging infectious diseases is very high after an outbreak and decreases drastically upon the containment of the disease. This can yield inadequate research and limited investment in gaining a full understanding of novel coronavirus management and prevention.

Conclusions
Independent of the outcome of the current COVID-19 outbreak, we believe that measures should be taken to encourage sustained research in the field.
The amino acid transporter Slc7a5 regulates the mTOR pathway and is required for granule cell development
Authors: Sokolov Aidan M , Holmberg Jennie C , Feliciano David M 
Published: August 2020

Abstract
Pathogenic mutations in the solute carrier family 7 member 5 (SLC7A5) gene, which encodes an amino acid transporter cause microcephaly and seizures, yet the mechanisms responsible for these phenotypes are unclear. Models have demonstrated that Slc7a5 deletion is embryonic lethal and that these embryos lack a fully formed telencephalon. This phenotype is similar to that of mammalian target of rapamycin (mTOR) protein kinase deletion or mTOR inhibition. Notably, in many cells, Slc7a5 import of amino acids is required to maintain mTOR activity. Slc7a5 is present within neurogenic regions during embryogenesis, is found in cultured neurons and can modulate neuronal electrophysiological properties. However, Slc7a5 is also highly expressed within endothelial cells of the blood–brain barrier where removal in conditional mice leads to severe behavioral defects and non-cell autonomous changes in neurons. Therefore, the extent that neural Slc7a5 is required for development is unclear. Here, subventricular zone neural stem cells that generate olfactory bulb granule cell neurons were electroporated with SLC7A5 or Slc7a5 short hairpin RNA encoding plasmids. Although early phases of neural development were unaltered, Slc7a5 knockdown effected late phases of GC dendrite maturation and survival. Slc7a5 knockdown also decreased mTOR pathway activity. Ras homolog enriched in brain, an mTOR activator, rescued the effect of Slc7a5 knockdown on mTOR pathway activity and dendrite arbors. The data presented here demonstrate that Slc7a5 is required for GC mTOR pathway activity, maturation and survival, which may help explain why Slc7a5 mutations prevent normal brain development and function.
Methodological Considerations for Assessing Immune Defense in Reproductive Females
Authors: Josefson Chloe C , Zohdy Sarah , Hood Wendy R 
Published: August 2020

SynopsisOne of the key foci of ecoimmunology is understanding the physiological interactions between reproduction and immune defense. To assess an immune challenge, investigators typically measure an immune response at a predetermined time point that was selected to represent a peak response. These time points often are based on the immunological responses of nonreproductive males. Problematically, these peaks have been applied to studies quantifying immune responses of females during reproduction, despite the fact that nonreproductive males and reproductive females display fundamentally different patterns of energy expenditure. Previous work within pharmacological research has reported that the response to the commonly-used antigen keyhole limpet hemocyanin (KLH) varies among individuals and between females and males. In this heuristic analysis, we characterize antibody responses to KLH in females with varying reproductive demands (nonreproductive, lactating, concurrently lactating, and pregnant). Serum was taken from one animal per day per group and assessed for general and specific Immunoglobulins (Igs) G and M. We then used regression analysis to characterize the antibody response curves across groups. Our results demonstrate that the antibody response curve is asynchronous among females with varying maternal demands and temporally differs from the anticipated peak responses reflected in standardized protocols. These findings highlight the importance of multiple sampling points across treatment groups for a more integrative assessment of how reproductive demand alters antibody responses in females beyond a single measurement.
Optimizing pricing and packing of variable-sized cargo
Authors: Martinez-Sykora A , So M C , Currie C S M , Bayliss C , Bennell J A 
Published: August 2020

Abstract
Organizations have successfully used dynamic pricing to optimize revenues for many years, where research and practice have mainly focused on applications with independent, discrete commodities; for example, an airline ticket. In this research we consider applications where the commodity is continuous and the value of the commodity available to sell depends on the combination of previously accepted demand. We focus on vehicle ferries, where the accepted vehicle bookings are packed in lanes in the ferry to leave a usable space for future bookings. Certain combinations of vehicles may result in areas of unusable space, which will affect future revenue. While this application is the focus of the paper, there are numerous industries that face similar challenges including freight and the sale of advertising on television and radio. In this paper, we simultaneously solve the pricing and resource utilization problem to optimality for a discrete set of product types and stochastic demand. Our approach combines a dynamic pricing model with a mixed-integer linear program to optimize the packing. We present results for real-world examples from the ferry industry and discuss extensions to the method to improve the selection of vehicle configurations.
Classification of Stable Solutions to a Non-Local Gelfand–Liouville Equation
Authors: Hyder Ali , Yang Wen 
Published: August 2020

Abstract
We study finite Morse index solutions to the non-local Gelfand–Liouville problem $$\begin{align*}& (-\Delta)^su=e^u\quad\textrm{in}\quad{{\mathbb{R}}^n}, \end{align*}$$for every $s\in (0,1)$ and $n&gt;2s$. Precisely, we prove non-existence of finite Morse index solutions whenever the singular solution $$\begin{align*} &u_{n,s}(x)=-2s\log|x|+\log \left(2^{2s}\frac{\Gamma(\frac{n}{2})\Gamma(1+s)}{\Gamma(\frac{n-2s}{2})}\right)\end{align*}$$is unstable.
From Shame to New Name: How Naming and Shaming Creates Pro-Government Militias
Authors: DiBlasi Lora 
Published: August 2020

AbstractResearchers have identified naming and shaming as a strategy used by the international community to reprimand state leaders for their repressive actions. Previous research indicates that there is variation in the success of this tactic. One reason for the heterogeneity in success is that leaders with an interest in repressing opposition but avoiding international condemnation have adapted their behavior, at least partially, to avoid naming and shaming. For instance, some states choose to create and utilize alternative security apparatuses, such as pro-government militias (PGMs), to carry out these repressive acts. Creating or aligning with PGMs allows leaders to distance themselves from the execution of violence while reaping the rewards of repression. This analysis explores this dynamic. In particular, I examine how naming and shaming by Amnesty International and the United Nations Commission on Human Rights influences the creation of PGMs to skirt future international condemnation by the offending state for all states from 1986 to 2000. I find that countries are more likely to create PGMs, especially informal PGMs, after their human rights abuses have been put in the spotlight by the international community.
Shaking Hands with the Internal Enemy: Democracy and Civil Conflict Settlement
Authors: Zarpli Omer 
Published: August 2020

AbstractHow does regime type affect the likelihood of negotiated settlements that end civil conflicts? A limited number of previous studies have offered divergent theories and mixed findings about whether democracy is an asset or a liability. I draw these disparate findings together and present a novel theory on why leaders under fully democratic and autocratic regimes may have a particularly difficult time in peacemaking, and how leaders in anocratic (hybrid) regimes are more likely to be successful in reaching negotiated settlements. Thus, I hypothesize that the relationship between regime type and the likelihood of conflict-resolution is inverted U-shaped. I test this hypothesis using data on all internal conflicts between 1946 and 2014, and find empirical support. The findings suggest that even if anocracies are more prone to the outbreak of civil wars as has been proposed by previous studies, they are also better at settling these conflicts.
Quantitative Determination of Ethyl Glucuronide and Ethyl Sulfate in Postmortem and Antemortem Whole Blood Using Phospholipid Removal 96-Well Plate and UHPLC–MS-MS
Authors: Sidqey Delvin , Liane Veronica Horpestad , Kristoffersen Lena 
Published: August 2020

Abstract
Postmortem ethanol formation is a well-known problem in forensic toxicology. Ethyl glucuronide (EtG) and ethyl sulfate (EtS) are ethanol metabolites that can be used to distinguish antemortem alcohol intake from postmortem formation of ethanol and in addition can be a helpful tool in assessment of the hip-flask defense. To an aliquot of 100 µL whole blood, internal standard (IS) and water was added before protein precipitation treatment (PPT) with ice-cold acetonitrile (ACN). The supernatants were filtered through a 96-well phospholipid removal plate, evaporated to dryness and reconstituted in 150 µL water/ACN/formic acid (FA). Identification of compounds was performed using multiple reaction monitoring (MRM) in negative mode. Gradient elution was performed on a C18 column with methanol (MeOH) and 0.1% FA. The run time was 4.5 min, and 0.5 µL was injected on an ultra-high-performance liquid chromatography–tandem mass spectrometry (UHPLC–MS-MS) instrument. Linearity was achieved (coefficient of determination (R2) ≥ 0.999) for EtG in the range of 0.089 to 22 mg/L (0.40–100 µM) and EtS 0.025 to 6.3 mg/L (0.20–50 µM). The limit of quantification (LOQ) was 0.067 mg/L (0.30 µM) for EtG and 0.019 mg/L (0.15 µM) for EtS. Between assay accuracy was –15% to 8% and precision reported as relative standard deviation (RSD) was ≤ 4.5%. Precision, estimated as the RSD of the concentration difference between results from two independent analyses of authentic whole blood samples, was ≤ 6.7%. Recovery was ≥ 61% for EtG and ≥ 77% for EtS and matrix effects (ME) were 99% to 103%. Method comparison was carried out with a previously used UHPLC–MS-MS method, and satisfactory agreement was achieved, and external proficiency testing control samples had z-score &lt; ± 1. The method has been used in routine work for more than 4 years analyzing about 6,000 antemortem and postmortem whole blood samples and has proven to be robust and reliable.
Improvement of Burn Scars Treated With Fractional Ablative CO2 Lasers—A Systematic Review and Meta-analysis Using the Vancouver Scar Scale
Authors: Mahar Patrick D , Spinks Anneliese B , Cleland Heather , Bekhor Philip , Waibel Jill S , Lo Cheng , Goodman Gregory 
Published: August 2020

Abstract
Fractional ablative CO2 laser is being used increasingly to treat burn scars; however, objective measures of outcome success vary widely. This systematic review and meta-analysis extracts and pools available data to assess the outcomes of patients with burn scars treated with fractional ablative CO2 laser. A search of MEDLINE, EMBASE, and the gray literature was performed. The review included studies that reported patients with a confirmed diagnosis of scarring as a result of a burn injury, who were treated with fractional ablative CO2 laser and whose progress was recorded using the Vancouver Scar Scale (VSS). Eight studies were included in the systematic review and meta-analysis. Treatment regimens varied amongst studies, as did patient outcomes. Pooled data revealed an average VSS improvement of 29% across 282 patients following fractional CO2 ablative laser treatment. Although the heterogeneity of treatment regimens across studies limits this systematic review’s ability to provide specific treatment recommendations, the overall trend towards improvement of burns scars treated with fractional CO2 laser based on the VSS encourages further exploration of this modality as a therapeutic tool.
Genomic Features of Parthenogenetic Animals
Authors: Jaron Kamil S , Bast Jens , Nowell Reuben W , Ranallo-Benavidez T Rhyker , Robinson-Rechavi Marc , Schwander Tanja 
Published: August 2020

AbstractEvolution without sex is predicted to impact genomes in numerous ways. Case studies of individual parthenogenetic animals have reported peculiar genomic features that were suggested to be caused by their mode of reproduction, including high heterozygosity, a high abundance of horizontally acquired genes, a low transposable element load, or the presence of palindromes. We systematically characterized these genomic features in published genomes of 26 parthenogenetic animals representing at least 18 independent transitions to asexuality. Surprisingly, not a single feature was systematically replicated across a majority of these transitions, suggesting that previously reported patterns were lineage-specific rather than illustrating the general consequences of parthenogenesis. We found that only parthenogens of hybrid origin were characterized by high heterozygosity levels. Parthenogens that were not of hybrid origin appeared to be largely homozygous, independent of the cellular mechanism underlying parthenogenesis. Overall, despite the importance of recombination rate variation for the evolution of sexual animal genomes, the genome-wide absence of recombination does not appear to have had the dramatic effects which are expected from classical theoretical models. The reasons for this are probably a combination of lineage-specific patterns, the impact of the origin of parthenogenesis, and a survivorship bias of parthenogenetic lineages.
From diet to hair and blood: empirical estimation of discrimination factors for C and N stable isotopes in five terrestrial mammals
Authors: Rioux Ève , Pelletier Fanie , St-Laurent Martin-Hugues 
Published: August 2020

AbstractCarbon and nitrogen stable isotope ratios are used widely to describe wildlife animal diet composition and trophic interactions. To reconstruct consumer diet, the isotopic differences between consumers and their diet items—called the trophic discrimination factor (TDF)—must be known. Proxies of diet composition are sensitive to the accuracy of TDFs. However, specific TDFs are still missing for many species and tissues because only a few controlled studies have been carried out on captive animals. The aim of this study was to estimate TDFs for hair and blood for carbon and nitrogen stable isotopes for caribou, moose, white-tailed deer, eastern coyote, and black bear. We obtained stable isotope ratios for diet items, hair, and blood samples, of 21 captive adult mammals. Diet–tissue discrimination factors for carbon in hair (∆ 13CLE) ranged from 0.96‰ to 3.72‰ for cervids, 3.01‰ to 3.76‰ for coyote, and 5.15‰ to 6.35‰ for black bear, while nitrogen discrimination factors (∆ 15N) ranged from 2.58‰ to 5.95‰ for cervids, 2.90‰ to 3.13‰ for coyote, and 4.48‰ to 5.44‰ for black bear. The ∆ 13CLE values in coyote blood components ranged from 2.20‰ to 2.69‰ while ∆ 15N ranged from 3.30‰ to 4.41‰. In caribou serum, ∆ 13CLE reached 3.34 ± 1.28‰ while ∆ 15N reached 5.02 ± 0.07‰. The TDFs calculated in this study will allow the evaluation of diet composition and trophic relationships between these five mammal species and will have important implications for the study of endangered caribou populations for which the use of noninvasive tissue sampling is highly relevant.
Low Abundance of Three Tick Species in the Piedmont of North Carolina
Authors: Seagle Madeline P , Vierling Maximilian R , Almeida Ryan J , Clary D Jacob , Hidell Will , Scott Erin V , Vargas Carlos , Smith Kevin G 
Published: August 2020

Abstract
Multiple species of ticks, including Ixodes scapularis (Say, Ixodida:Ixodidae), Amblyomma americanum (L., Ixodida:Ixodidae), and Dermacentor variabilis (Say, Ixodida:Ixodidae), occur in high and increasing abundance in both the northeast and southeast United States. North Carolina is at the nexus of spread of these species, with high occurrence and abundance of I. scapularis to the north and A. americanum to the south. Despite this, there are few records of these species in the Piedmont of North Carolina, including the greater Charlotte metropolitan area. Here, we update the known occurrence and abundance of these species in the North Carolina Piedmont. We surveyed for ticks using cloth drags, CO2 traps, and leaf litter samples at a total of 79 sites within five locations: Mecklenburg County, South Mountains State Park, Stone Mountain State Park, Duke Forest, and Morrow Mountain State Park, all in North Carolina, during the late spring, summer, and fall seasons of 2019. From these surveys, we had only 20 tick captures, illuminating the surprisingly low abundance of ticks in this region of North Carolina. Our results indicate the possibility of underlying habitat and host factors limiting tick distribution and abundance in the North Carolina Piedmont.
Determination of reproducibility of end-exhaled breath-holding in stereotactic body radiation therapy
Authors: Sasaki Motoharu , Ikushima Hitoshi , Sakuragawa Kanako , Yokoishi Michihiro , Tsuzuki Akira , Sugimoto Wataru 
Published: August 2020

ABSTRACT
Methods to evaluate the positional reproducibility of breath-hold irradiation mostly require manual operation. The purpose of this study is to propose a method to determine the reproducibility of breath-hold irradiation of lung tumors between fractions using non-artificial methods. This study included 13 patients who underwent terminal exhaled breath-hold irradiation for primary and metastatic lung cancer. All subjects received a prescribed dose of 60 Gy/8 fractions. The contours of the gross tumor volume (GTV) were extracted by threshold processing using treatment-planning computed tomography (CT) and cone-beam CT (CBCT), which was done just before the beginning of the treatment. The method proposed in this study evaluates the dice similarity coefficient (DSC) and Hausdorff distance (HD) by comparing two volumes, the GTVCTS (GTV obtained from treatment-planning CT) and GTVCBCT (GTV obtained from CBCT). The reference contours for DSC and HD are represented by GTVCTS. The results demonstrated good visual agreement for cases with a DSC of ~0.7. However, apparent misalignment occurred when the DSC was &lt;0.5. HD was &gt;2 mm in 3 out of 13 cases, and when the DSC was ~0.7, the HD was ~1 mm. In addition, cases with greater HD also demonstrated more significant variability. It was found that the DSC and HD evaluation methods for the positional reproducibility of breath-hold irradiation proposed in this study are straightforward and can be performed without the involvement of humans. Our study is of extreme significance in the field of radiation studies.
Suppressed ABA signal transduction in the spike promotes sucrose use in the stem and reduces grain number in wheat under water stress
Authors: Zhang Zhen , Huang Jing , Gao Yanmei , Liu Yang , Li Jinpeng , Zhou Xiaonan , Yao Chunsheng , Wang Zhimin , Sun Zhencai , Zhang Yinghua 
Published: August 2020

AbstractWater stress is a primary trigger for reducing grain number per spike in wheat during the reproductive period. However, under stress conditions, the responses of plant organs and the interactions between them at the molecular and physiological levels remain unclear. In this study, when water stress occurred at the young microspore stage, RNA-seq data indicated that the spike had 970 differentially expressed genes, while the stem, comprising the two internodes below the spike (TIS), had 382. Abscisic acid (ABA) signal transduction genes were down-regulated by water stress in both these tissues, although to a greater extent in the TIS than in the spike. A reduction in sucrose was observed, and was accompanied by increases in cell wall invertase (CWIN) and sucrose:sucrose 1-fructosyl-transferase (1-SST) activities. Hexose and fructan were increased in the TIS but decreased in the spike. ABA was increased in the spike and TIS, and showed significant positive correlation with CWIN and 1-SST activities in the TIS. Overall, our results suggest that water stress induces the conversion of sucrose to hexose by CWIN, and to fructan by 1-SST, due to increased down-regulation of ABA signal transduction related-genes in the TIS; this leads to deficient sucrose supply to the spike and a decrease in grain number.
Ancestral function of the phytochelatin synthase C-terminal domain in inhibition of heavy metal-mediated enzyme overactivation
Authors: Li Mingai , Barbaro Enrico , Bellini Erika , Saba Alessandro , Sanità di Toppi Luigi , Varotto Claudio 
Published: August 2020

AbstractPhytochelatin synthases (PCSs) play essential roles in detoxification of a broad range of heavy metals in plants and other organisms. Until now, however, no PCS gene from liverworts, the earliest branch of land plants and possibly the first one to acquire a PCS with a C-terminal domain, has been characterized. In this study, we isolated and functionally characterized the first PCS gene from a liverwort, Marchantia polymorpha (MpPCS). MpPCS is constitutively expressed in all organs examined, with stronger expression in thallus midrib. The gene expression is repressed by Cd2+ and Zn2+. The ability of MpPCS to increase heavy metal resistance in yeast and to complement cad1-3 (the null mutant of the Arabidopsis ortholog AtPCS1) proves its function as the only PCS from M. polymorpha. Site-directed mutagenesis of the most conserved cysteines of the C-terminus of the enzyme further uncovered that two twin-cysteine motifs repress, to different extents, enzyme activation by heavy metal exposure. These results highlight an ancestral function of the PCS elusive C-terminus as a regulatory domain inhibiting enzyme overactivation by essential and non-essential heavy metals. The latter finding may be relevant for obtaining crops with decreased root to shoot mobility of cadmium, thus preventing its accumulation in the food chain.