bluuebunny/crossref_metadata_2025_split huggingface dataset:
Light-inducedpsbAtranslation in plants is triggered by photosystem II damage via an assembly-linked autoregulatory circuit
Authors: Chotewutmontri Prakitchai , Barkan Alice 
Published: April 2020

AbstractThe D1 reaction center protein of Photosystem II (PSII) is subject to light-induced damage. Degradation of damaged D1 and its replacement by nascent D1 are at the heart of a PSII repair cycle, without which photosynthesis is inhibited. In mature plant chloroplasts, light stimulates the recruitment of ribosomes specifically topsbAmRNA to provide nascent D1 for PSII repair, and also triggers a global increase in translation elongation rate. The light-induced signals that initiate these responses are unclear. We present action spectrum and genetic data indicating that the light-induced recruitment of ribosomes topsbAmRNA is triggered by D1 photodamage, whereas the global stimulation of translation elongation is triggered by photosynthetic electron transport. Furthermore, mutants lacking HCF136, which mediates an early step in D1 assembly, exhibit constitutively highpsbAribosome occupancy in the dark, and differ in this way from mutants lacking PSII for other reasons. These results, together with the recent elucidation of a thylakoid membrane complex that functions in PSII assembly, PSII repair andpsbAtranslation, suggest an autoregulatory mechanism in which the light-induced degradation of D1 relieves repressive interactions between D1 and translational activators in the complex. We suggest that the presence of D1 in this complex coordinates D1 synthesis with the need for nascent D1 during both PSII biogenesis and PSII repair in plant chloroplasts.Significance StatementPhotosystem II (PSII) harbors the water-splitting activity underlying oxygenic photosynthesis. The PSII reaction center protein D1 is subject to photodamage and must be replaced with nascent D1 to maintain photosynthetic activity. How new D1 synthesis is coordinated with D1 damage has been a long-standing question. Our results clarify the nature of the light-induced signal that activates D1 synthesis for PSII repair in plants, and suggest an autoregulatory mechanism in which degradation of damaged D1 relieves a repressive interaction between D1 and translational activators in a complex that functions in PSII assembly and repair. This proposed mechanism comprises a responsive switch that couples D1 synthesis to need for D1 during PSII biogenesis and repair.
Interplay between Anakonda, Gliotactin and M6 for tricellular junction assembly and anchoring of septate junctions inDrosophilaepithelium
Authors: de Bournonville Thomas Esmangart , Le Borgne Roland 
Published: April 2020

SummaryIn epithelia, Tricellular junctions (TCJs) serve as pivotal sites for barrier function and integration of both biochemical and mechanical signals. While essential for tissue homeostasis, TCJ assembly, composition and links to adjacent bicellular junctions (BCJs) remain poorly understood. Here we have characterized the assembly of TCJs within the plane of adherens junctions (tAJ) and the plane of septate junctions (tSJ) inDrosophilaand report that their formation is spatiotemporally decoupled. The assembly and stabilization of previously described tSJ components Anakonda (Aka) and Gliotactin (Gli) as well as the newly reported tSJ proteolipid protein M6, is shown to be a complex process. Aka and M6, whose localization is interdependent, act upstream to locate Gli. In turn, Gli stabilizes Aka at tSJ. Those results unravel a previous unknown role of M6 at tSJ and a tight interplay between tSJ components to assemble and maintain tSJs. In addition, tSJ components are not only essential at vertex as we found that loss of tSJ integrity also induces micron-length bicellular SJs deformations that are free of tensile forces. This phenotype is associated with the disappearance of SJ components at tricellular contacts, indicating that bSJ are no longer connected to tSJs. Reciprocally, SJ components are in turn required to restrict the localization of Aka and Gli at vertex. We propose that tSJs function as pillars to anchor bSJs to ensure the maintenance of tissue integrity inDrosophilaproliferative epithelia.
The inflammasome components NLRP3 and ASC act in concert with IRGM to rearrange the Golgi during viral infections
Authors: Daussy Coralie F. , Monard Sarah C. , Guy Coralie , Muñoz-González Sara , Chazal Maxime , Anthonsen Marit W. , Jouvenet Nolwenn , Henry Thomas , Dreux Marlène , Meurs Eliane F. , Hansen Marianne Doré 
Published: April 2020

AbstractHepatitis C virus (HCV) infection triggers Golgi fragmentation through the Golgi-resident protein immunity-related GTPase M (IRGM). Here, we report the role of NLRP3 and ASC, two inflammasome components, in the initial events leading to this fragmentation. We show that ASC resides at the Golgi with IRGM at homeostasis. Upon infection, ASC dissociates from both IRGM and Golgi and associates with HCV-induced NLRP3. NLRP3 silencing inhibits Golgi fragmentation. ASC silencing disrupts the Golgi structure in both control and infected cells and reduces the localization of IRGM at the Golgi. Silencing IRGM cannot totally restore the Golgi structure. These data highlight a role for ASC, upstream of the formation of the inflammasome, in regulating IRGM through its control on the Golgi. A similar mechanism occurs in response to Nigericin or infection with Zika virus (ZIKV). We propose a model for a newly ascribed function of the inflammasome components in Golgi structural remodeling.
LncRNAs Landscape in the patients of primary gout by Microarray Analysis
Authors: Qing Yu-Feng , Zheng Jian-Xiong , Tang Yi-Ping , Dai Fei , Dong Zeng-Rong , Zhang Quan-Bo 
Published: April 2020

AbstractTo determine the differential profiles of long non-coding RNAs (lncRNAs) in Peripheral blood mononuclear cells (PBMCs) among acute gout (AG) patients, intercritical gout (IG) patients and healthy control subjects, and to explore the specific biomarkers for acute gout diagnosis and treatment in future. Human lncRNA microarrays were used to identify the differentially expressed lncRNAs and mRNAs in primary AG (n=3), IG (n=3) and healthy subjects (n=3). For comparison Bioinformatics analyses were performed to predict the roles of abberrantly expressed lncRNAs and mRNAs. Quantitative real-time polymerase chain reaction (qRT-PCR) was applied to validate the results in 32 AG, 32 IG patients and 32 healthy control subjects. A receiver operating characteristic (ROC) curve was constructed to evaluate the diagnostic value of the lncRNAs identified in gout. The microarray analysis identified 3421 and 1739 differentially expressed lncRNAs, 2240 and 1027 differentially expressed mRNAs in AG and IG (fold change>1.5, P<0.05; respectively), respectively. qRT-PCR results of 9 dysregulated genes were consistent with the microarray data. The bioinformatic analysis indicated that the differentially expressed lncRNAs regulated the abnormally expressed mRNAs, which were involved in the pathogenesis of gout through several different pathways. The expression levels of TCONS_00004393 and ENST00000566457 were significantly increased in the AG group than those in the IG group or healthy subjects (P<0.01, respectively). Moreover, the areas under the ROC curve were 0.955 and 0.961 for TCONS_00004393 and ENST00000566457,respectively. Our results provide novel insight into the mechanisms of the primary gout, and reveal that TCONS_00004393 and ENST00000566457 might be as candidate diagnostic biomarkers and targets for the treatment of acute gout.
Efficient Dilution-to-Extinction isolation of novel virus-host model systems for fastidious heterotrophic bacteria
Authors: Buchholz Holger H. , Michelsen Michelle , Bolaños Luis M. , Browne Emily , Allen Michael J. , Temperton Ben 
Published: April 2020

AbstractMicrobes and their associated viruses are key drivers of biogeochemical processes in marine and soil biomes. While viruses of phototrophic cyanobacteria are well-represented in model systems, challenges of isolating marine microbial heterotrophs and their viruses have hampered experimental approaches to quantify the importance of viruses in nutrient recycling. A resurgence in cultivation efforts has improved the availability of fastidious bacteria for hypothesis testing, but this has not been matched by similar efforts to cultivate their associated bacteriophages. Here, we describe a high-throughput method for isolating important virus-host systems for fastidious heterotrophic bacteria that couples advances in culturing of hosts with sequential enrichment and isolation of associated phages. Applied to six monthly samples from the Western English Channel, we first isolated one new member of the globally dominant bacterial SAR11 clade and three new members of the methylotrophic bacterial clade OM43. We used these as bait to isolate 117 new phages including the first known siphophage infecting SAR11, and the first isolated phage for OM43. Genomic analyses of 13 novel viruses revealed representatives of three new viral genera, and infection assays showed that the viruses infecting SAR11 have ecotype-specific host-ranges. Similar to the abundant human-associated phage ΦCrAss001, infection dynamics within the majority of isolates suggested either prevalent lysogeny or chronic infection, despite a lack of associated genes; or host phenotypic bistability with lysis putatively maintained within a susceptible subpopulation. Broader representation of important virus-host systems in culture collections and genomic databases will improve both our understanding of virus-host interactions, and accuracy of computational approaches to evaluate ecological patterns from metagenomic data.
Multi-tissue patterning drives anterior morphogenesis of theC. elegansembryo
Authors: Grimbert Stephanie , Mastronardi Karina , Christensen Ryan , Law Christopher , Fay David , Piekny Alisa 
Published: April 2020

AbstractComplex structures derived from multiple tissue types are challenging to studyin vivo, and our knowledge of how cells from different tissues are coordinated is limited. Model organisms have proven invaluable for improving our understanding of how chemical and mechanical cues between cells from two different tissues can govern specific morphogenetic events. Here we usedCaenorhabditis elegansas a model system to show how cells from three different tissues are coordinated to give rise to the anterior lumen. This poorly understood process has remained a black box for embryonic morphogenesis. Using various microscopy and software approaches, we describe the movements and patterns of epidermal cells, neuroblasts and pharyngeal cells that contribute to lumen formation. The anterior-most pharyngeal cells (arcade cells) may provide the first marker for the location of the future lumen and facilitate the patterning of the surrounding neuroblasts. These neuroblast patterns control the rate of migration of the anterior epidermal cells, whereas the epidermal cells ultimately reinforce and control the position of the future lumen, as they must join with the pharyngeal cells for their epithelialization. Our studies are the first to characterize anterior morphogenesis inC. elegansin detail and should lay the framework for identifying how these different patterns are controlled at the molecular level.
Photoinduced DNA Lesions in Dormant Bacteria. The Peculiar Route Leading to Spore Photoproduct Unraveled by Multiscale Molecular Dynamics
Authors: Francés-Monerris Antonio , Hognon Cécilia , Douki Thierry , Monari Antonio 
Published: April 2020

Some bacterial species enter a dormant state in the form of spores to resist to unfavorable external conditions. Spores are resistant to a wide series of stress agents, including UV radiation, and can last for tens to hundreds of years. Due to the suspension of biological functions such as DNA repair, they accumulate DNA damage upon exposure to UV radiation. Differently from active organisms, the most common DNA photoproduct in spores are not cyclobutane pyrimidine dimers, but rather the so-called spore photoproduct. This non-canonical photochemistry results from the dry state of DNA and the binding to small acid soluble proteins that drastically modify the structure and photoreactivity of the nucleic acid. In this contribution, we use multiscale molecular dynamics simulations including extended classical molecular dynamics and QM/MM biased dynamics to elucidate the coupling of electronic and structural factors leading to this photochemical outcome. In particular, we rationalize the well-described impact of the peculiar DNA environment found in spores on the favored formation of the spore photoproduct, given the small free energy barrier found for this path. Meanwhile, the specific organization of spore DNA precludes the photochemical path leading to cyclobutane pyrimidine dimers formation.TOC GRAPHICS
Abnormal features of human self-domestication in bipolar disorder
Authors: Benítez-Burraco Antonio , Hansen Ethan 
Published: April 2020

AbstractBipolar disorder (BD) is a severe mental condition characterized by episodes of elevated mood and depression. Being a heritable condition, it features a complex genetic architecture, and it is not still clear how genes contribute to the onset and course of the disease. In this paper we adopted an evolutionary-genomic approach to this condition, focusing on changes occurring during human evolution as a source of our distinctive cognitive and behavioral phenotype. We show clinical evidence that the BD phenotype can be construed as an abnormal presentation of the human self-domestication phenotype. We further demonstrate that candidate genes for BD significantly overlap with candidates for mammal domestication, and that this common set of genes is enriched in functions that are important for the BD phenotype, especially neurotransmitter homeostasis. Finally, we show that candidates for domestication are differentially expressed in brain regions involved in BD pathology, particularly, the hippocampus and the prefrontal cortex. Overall, this link between domestication and BD should facilitate a better understanding of the BD etiopathology.
Validation of satisfaction with life scale in the Indian manufacturing sector
Authors: Dahiya Rinki , Rangnekar Santosh 
Published: April 2020

PurposeIn the modern world, life satisfaction has become a quintessential construct for large-scale organizations, and a valid measure to evaluate employee life satisfaction is required. Therefore, this study aims to evaluate the psychometric properties of the satisfaction with life scale (SWLS) in a sample of employees working in the Indian manufacturing sector.Design/methodology/approachSurvey method was used for data collection among a sample of 404 employees. Further, the psychometric properties of SWLS were evaluated with reference to reliability and validity assessment (face, content and construct validity).FindingsThe results were found to be significant for SWLS to assess the life satisfaction of employees working in manufacturing organizations in India.Originality/valueThis study attempts to validate SWLS among employees, which is a novel contribution in the field. Also, it suggests that effective application of SWLS can help in identifying and understanding the work-related factors associated with life satisfaction.
Characterization of path loss model for wireless communication channel modelling
Authors: Sirdeshpande Nandakishor , Udupi Vishwanath 
Published: April 2020

PurposeWireless communication channel provides a wide area of applications in the field of communication, distributed sensor network and so on. The prominence of the wireless communication channel is because of its robust nature and the sustainability for the precise ranging and the localization. The precision and accuracy of the wireless communication channel largely depend on the localization. The development of the wireless communication channel with improved benefits needs the accurate channel model.Design/methodology/approachThis paper characterizes the tangential path loss model in the WINNER based wireless communication channel model. The measurements taken in the WINNER channel model are compared with the tangential path loss characterized WINNER Channel model.FindingsThe model operates well over the varying antenna orientations, measurement condition and the propagation condition. The proposed tangential path loss model is performing well over the various outdoor scenarios.Originality/valueThe proposed characterization shows change in the small-scale parameters (SSP), such as power, delay, angle of arrival and angle of departure as well as the large-scale parameters (LSP), such as RMS delay spread, shadowing, path loss and Ricean factor associated with the model.
Impostor syndrome: an integrative framework of its antecedents, consequences and moderating factors on sustainable leader behaviors
Authors: K.H. Aparna , Menon Preetha 
Published: April 2020

PurposeThe purpose of this paper is to integrate impostor syndrome and leadership research to identify antecedents of impostor syndrome, their impact on sustainable leader behaviors. The paper also postulates the moderating effect of mindfulness and leader member exchange on impostor syndrome and sustainable leader behaviors, respectively.Design/methodology/approachThe paper relies on an integrative approach of literature review on impostor syndrome and leadership. After identifying gaps in impostor syndrome research and its intersectionality with the constructs of contextual leadership theory, an integrative conceptual framework was formulated incorporating antecedents, consequences and moderators of impostor syndrome.FindingsThree antecedents of impostor syndrome were identified from the literature, namely, gender, family/social role expectation and personality traits. Additionally, this paper also unearths contextual factors as yet another antecedent to impostor syndrome. Negative impact of impostor syndrome on leader behaviors such as managerial decision-making, innovative work behavior (IWB) and organizational citizenship behavior (OCB) were established by connecting those to the three primary characteristics of impostor syndrome, namely, low self-efficacy, fear of failure and perceived fraudulence, respectively. Finally, the paper also posits the moderating role of leader member exchange and mindfulness and proposes mindfulness training as an effective intervention for impostor syndrome.Research limitations/implicationsThis being a conceptual paper will benefit from empirical studies that corroborate theoretical posits. The scope of studying the effect of impostor syndrome on sustainable leader behavior was limited to three major variables, namely, managerial decision-making, IWB and OCB. Thus, it calls for a more elaborate model of impostor syndrome including other relevant leader behaviors.Practical implicationsThe model when applied in organizational context addresses the need for mindfulness training to reduce the effect of impostor syndrome among leaders. Leaders will exhibit sustainable behaviors when provided with the right kind of training.Originality/valueThe study attempts to integrate the two independent constructs, impostor syndrome and leadership to establish a novel and meaningful connection and throws light to the unaddressed antecedents, consequences and moderators of its impact on sustainable leader behaviors. From learning and development practitioners’ perspective, it also signifies the effectiveness of mindfulness training among employees’ personal and professional development.
An investigation into the erroneous access and egress behaviours of building users and their impact upon building performance
Authors: Dixon Charlotte , Edwards David John , Mateo-Garcia Monica , Lai Joseph , Thwala Wellington Didibhuku Didibhuku , Shelbourn Mark 
Published: April 2020

Purpose
This study aims to investigate the behaviour of building users and how this impacts upon building energy performance. Specifically, the work examines the behavioural traits of able-bodied users of a large higher education building who erroneously access and egress the building using doorways intended for disabled users.

Design/methodology/approach
An inductive methodological approach is adopted that uses grounded theory to devise new insights into building users’ access and egress habits. Structured interviews are conducted to collect primary data from 68 building users of a large educational building over a four-week period. Responses to questions posed provide the basis for a tabularisation of behavioural traits.

Findings
Reasons for able-bodied building users’ preferences to using disabled access are identified and discussed; these are thematically grouped under the headings of apathy, convenience, emergency, ergonomics, ignorance and phobia. Building upon these findings, the research then offers insights into the approaches that could be adopted to change the erroneous behaviours. These approaches include education of building users on the impact their behaviour has upon building performance and environmental pollution, more stringent regulation to penalise repeat offenders and changes to building entrance design using obtrusive (i.e. radio frequency identification tags) and unobstrusive control measures (i.e. a second entrance doorway or slower opening mechanism).

Originality/value
To the best of the author’s knowledge, this study is the first of its kind to investigate the rationale for able-bodied building users erroneously using disabled persons’ access and egress doorways within a building, which as a consequence, inadvertently reduces the building’s environmental performance.
Acceptance of Islamic financial technology (FinTech) banking services by Malaysian users: an extension of technology acceptance model
Authors: Shaikh Imran Mehboob , Qureshi Muhammad Asif , Noordin Kamaruzaman , Shaikh Junaid Mehboob , Khan Arman , Shahbaz Muhammad Saeed 
Published: April 2020

Purpose
This paper aims to examine the determinants that influence bank users’ acceptance for Islamic financial technology (FinTech) services by extending the technology acceptance model (TAM) in the Malaysian context.

Design/methodology/approach
The survey was conducted using convenience sampling. Moreover, 205 responses were gathered from users of the Islamic bank. On the same note, the literature on determinants of Islamic FinTech acceptance and TAM was reviewed as well in a bid to contribute to the factors that are instrumental in determining the acceptance of FinTech services.

Findings
Findings of the study reveal that Islamic FinTech’s services acceptance is determined by perceived ease of use, perceived usefulness and also by another variable, which is consumer innovativeness (CI). On the contrary other factors, self-efficacy and subjective norms are found not to be influential in determining Islamic FinTech’s acceptance by Islamic banking users.

Originality/value
TAM is extended in the context of Islamic FinTech. A new variable, namely, CI is tested using TAM. CI is yet to be tested, therefore, this paper will be a useful reference for the policymakers, academicians and future researchers.