bluuebunny/crossref_metadata_2025_split huggingface dataset:
Rapid identification and characterization of genetic loci for defective kernel in bread wheat
Authors: Fu Chao , Du Jiuyuan , Tian Xiuling , He Zhonghu , Fu Luping , Wang Yue , Xu Dengan , Xu Xiaoting , Xia Xianchun , Zhang Yan , Cao Shuanghe 
Published: November 2019

Abstract
Background Wheat is a momentous crop and feeds billions of people in the world. The improvement of wheat yield is very important to ensure world food security. Normal development of grain is the essential guarantee for wheat yield formation. The genetic study of grain phenotype and identification of key genes for grain filling are of great significance upon dissecting the molecular mechanism of wheat grain morphogenesis and yield potential. Results Here we identified a pair of defective kernel (Dek) isogenic lines, BL31 and BL33, with plump and shrunken mature grains, respectively, and constructed a genetic population from the BL31/BL33 cross. Ten chromosomes had higher frequency of polymorphic single nucleotide polymorphism (SNP) markers between BL31 and BL33 using Wheat660K chip. Totally 783 simple sequence repeat (SSR) markers were chosen from the above chromosomes and 15 of these were integrated into two linkage groups using the genetic population. Genetic mapping identified three QTL, QDek.caas-3BS.1, QDek.caas-3BS.2 and QDek.caas-4AL, explaining 14.78 - 18.17%, 16.61 - 21.83% and 19.08 - 28.19% of phenotypic variances, respectively. Additionally, five polymorphic SNPs from Wheat660K were successfully converted into cleaved amplified polymorphic sequence (CAPS) markers and enriched the target regions of the above QTL. Biochemical analyses revealed that BL33 has significantly higher grain sucrose contents at filling stages and lower mature grain starch contents than BL31, indicating that the Dek QTL may be involved in carbohydrate metabolism. As such, the candidate genes for each QTL were determined according to International Wheat Genome Sequence Consortium (IWGSC) RefSeq v1.0. Conclusions Overall, we achieved a rapid QTL mapping and reasonable predication of causal genes for Dek, laying foundation to conduct fine mapping and dissect the regulatory mechanism underlying Dek trait in wheat.
Transcriptomic analysis of α-synuclein knockdown after T3 spinal cord injury in rats
Authors: Zeng Hong , Yu Bao-fu , Liu Nan , Yang Yan-yan , Xing Hua-yi , Liu Xiao-xie , Zhou Mouwang 
Published: November 2019

Abstract
Abstract
Background
Endogenous α-synuclein (α-Syn) is involved in many pathophysiological processes in the secondary injury stage after acute spinal cord injury (SCI), and the mechanism governing these functions has not been thoroughly elucidated to date. This research aims to characterize the effect of α-Syn knockdown on transcriptional levels after SCI and to determine the mechanisms underlying α-Syn activity based on RNA-seq.
Result
The establishment of a rat model of lentiviral vector-mediated knockdown of α-Syn in Sprague-Dawley rats with T3 spinal cord contusion (LV_SCI group). The results of the RNA-seq analysis showed that there were 337 differentially expressed genes (DEGs) between the SCI group and the LV_SCI group, and 153 DEGs specific to LV_SCI between the (SCI vs LV_SCI) and (SCI vs CON) comparisons . The top 20 biological transition terms were identified by Gene ontology (GO) analysis. The Kyoto Gene and Genomic Encyclopedia (KEGG) analysis showed that the LV_SCI group significantly upregulated the cholinergic synaptic & nicotine addiction and the neuroactive ligand receptor interaction signaling pathway. Enriched chord analysis analyzes key genes. Further cluster analysis, gene and protein interaction network analysis and RT-qPCR results showed that Chrm2 and Chrnb2 together significantly in both pathways. The proliferation of muscarinic cholinergic receptor subtype 2 (Chrm2) and nicotinic cholinergic receptor subtype β2 (Chrnb2), and the neurogenesis were elevated in the injury site of LV_SCI group by immunofluorescence. Further by subcellular localization, the LV_SCI group enhanced the expression of Chrnb2 at the cell membrane.
Conclusion
Knockdown of α-Syn after SCI enhance motor function and promote neurogenesis probably through enhancing cholinergic signaling pathways and neuroreceptor interactions. This study not only further clarifies the understanding of the mechanism of knockdown of α-Syn on SCI but also helps to guide the treatment strategy for SCI.
Three Easily-Implementable Changes Reduce Median Door-To-Needle Time for Intravenous Thrombolysis by 23 Minutes
Authors: Tran Demi , Zhu Zhu , Shafie Mohammad , Abcede Hermelinda , Stradling Dana , Yu Wengui 
Published: November 2019

Abstract
Background: The benefit of intravenous thrombolysis (IVT) for acute ischemic stroke is time dependent. Despite great effort, the median door-to-needle time (DNT) was 60 minutes at the United States stroke centers. We investigated the effect of a simple quality improvement initiative on DNT for IVT.
Methods: This is a single-center study of patients treated with IVT between 2013 and 2017. A simple quality improvement initiative was implemented in January 2015 to allow the Stroke team to manage hypertension in the emergency room, to make decision for IVT before getting blood test results unless patients were taking oral anticoagulants, and to give IVT in the CT suite. Baseline characteristics, DNT and outcomes at hospital discharge were compared between pre- and post-intervention groups.
Results: Ninety and 136 patients were treated with IVT in pre- and post-intervention groups, respectively. The rate of IVT was significantly higher in the post-intervention group (20% vs. 14.4%, p=0.007). The median DNT with interquartile range (IQR) was reduced significantly by 23 minutes (63[53-81] vs. 40[29-53], p<0.001) with more patients in the post-intervention group receiving IVT within 60 minutes (81.6% vs. 46.7%) and 45 minutes (64.0% vs.17.8%). There was no significant difference in symptomatic intracerebral hemorrhage rate (1.5% vs. 1.1%), functional independence at discharge (modified Rankin Scale 0-1, 29.4% vs. 23.3%), and hospital mortality (7.4% vs. 6.7%) between the 2 groups.
Conclusions: Three easily-implementable quality improvement initiative increases IVT rate and reduces DNT significantly without increasing the rate of IVT-related complications in our comprehensive stroke center.
Sudden intrauterine unexplained death: time to adopt uniform postmortem investigative guidelines?
Authors: Lavezzi Anna , Piscioli Francesco , Pusiol Teresa , Jorizzo Gianfranco , Ferrero Stefano 
Published: November 2019

Abstract
Background: Worldwide approximately 2.6 million are stillborn, mostly occurring in developing countries. In the great part these deaths are inexplicable. The evenness and standardisation of the diagnostic criteria are prerequisites to understand their pathogenesis. The core goal of this article is to propose new evidence based investigative post-mortem guidelines that should be adopted in all the Institutions especially when a fetal death, after a routine autopsy procedure, is diagnosed as “unexplained”. The proposed protocol is mainly focused on the anatomopathological examination of the autonomic nervous system and in particular of the brainstem where the main centers that control vital functions are located.

Methods: Updated investigative guidelines for the examination of unexplained stillbirths, prevalently focused on the histological examination of the brainstem, where the main centers that are involved in monitoring the vital functions are located, are here presented. A section of this protocol concerns the Immunohistochemical evaluation of specific functional markers such as the neuronal nuclear antigen, nicotinic acetylcholine receptors, serotonin, orexin, apoptosis and gliosis. The important role of risk factors, having regard in particular to maternal smoking and air pollution is also contemplated in these guidelines.
Results: Specific morphological and/or functional alterations of vital brainstem structures have been found with high incidence in over 100 cases of unexplained fetal death sent to the “Lino Rossi Research Center” of the Milan University according to the Italian law. These alterations were rarely detected in a group of control cases.
Conclusions: We hope this protocol can be adopted in all the Institutions notably for the examination of unexplained fetal deaths, in order to make uniform investigations. This will lead to identify a plausible explanation of the pathogenetic mechanism behind the unexplained fetal deaths and to design preventive strategies to decrease the incidence of these very distressing events for both parents and clinicians.
The effect of Xuanbai Chengqi decoction&nbsp;on patients with pneumonia-derived sepsis: study protocol for a randomized controlled trial
Authors: Jun Zhang , Yu Yi , Zheng Bojun , Jing Huang 
Published: November 2019

Abstract
INTRODUCTION We wish to investigate the efficacy of Xuanbai Chengqi decoction (XCD) on management of pneumonia-derived sepsis.METHODS AND ANALYSIS A single-blind, randomised controlled clinical trial will be conducted involving 90 patients with pneumonia-derived sepsis. Participants will be randomised at a 1:1 ratio to receive XCD (experimental arm) or the same amount of saline treatment (control arm). The intervention will comprise one session/day for 1 week. The primary outcomes will be 28-day mortality, and levels of pro-inflammatory cytokines in bronchoalveolar lavage fluid and serum 1, 3 and 7 days after treatment completion with respect to baseline levels. Secondary outcomes will be the symptom score of traditional Chinese medicine, duration of parenteral nutrition, and prevalence of complications. Measurements will be taken at baseline, 1, 3 and 7 days during the intervention, after 28 days after completing the intervention. Adverse events between arms will be evaluated.
Transcriptome analysis of harvest blueberries (Vaccinium corymbosum ‘Duke’) in response to cold stress
Authors: Zhang Fan , Ji ShuJuan , Wei BaoDong , Cheng Shunchang , Hao Jia , Wang YaJuan , Wang SiYao , Zhou Qian 
Published: November 2019

Abstract
Background Blueberry ( Vaccinium spp. ) is a small berry with high economic value. Although cold storage can extend the storage time of blueberry to more than 60 days, it leads to chilling injury (CI) displayed as pedicle pits; and the samples of 0°C-30 days was the critical point of chilling injury (CI). However, little is known about the mechanism and the molecular basis response to cold stress in blueberry have not been explained definitely.Methods To comprehensively reveal the CI mechanisms in response to cold stress, we performed high-throughput RNA Seq analysis to investigate the gene regulation network in blueberries at different storage temperatures (20°C and 0°C) in this study. At the same time, the pitting and decay rate, electrolyte leakage, malondialdehyde (MDA) and proline content were also measured.Results High-throughput transcriptome sequences were assembled into 35,060 unique transcripts with an N50 length of 1,348bp. A total of 1,167 up-regulated and 685 down-regulated differentially expressed genes (DEGs) were annotated and classified between the CI group and control. Forty-five cold-induced transcription factor (TF) families containing 1,023 TFs were identified. The DEGs indicated in biological processes such as stress responses; cell wall metabolism; abscisic acid, gibberellin, membrane lipid, energy metabolism, cellular components, and molecular functions were significantly responsed to low temperature storage. The transcriptional level of 40 DEGs were verified by qRT-PCR.Conclusions The harvest cold storage leads serious CI in blueberries, which substantially decreases the quality, storability and consumer acceptance. In our research, the physiological changes during the cold storage were determined; the biological processes such as stress responses, hormone metabolic processes were significantly affected by CI. Overall, the results obtained here are valuable for preventing pitting under cold storage and could serve as new targets for enhancing blueberry fruit post harvest storage.
Lapatinib in combination with capecitabine versus continued use of trastuzumab in breast cancer patients with trastuzumab-resistance: a real-world study
Authors: Yang fan , Huang Xiang , Sun Chunxiao , Li Jianbin , Wang Biyun , Yan Min , Jin Feng , Wang Haibo , Zhang Jin , Fu Peifen , Zeng Tianyu , Wang Jian , Li Wei , Li Yongfei , Yang Mengzhu , Li Jun , Wu Hao , Fu Ziyi , Yin Yongmei , jiang zefei 
Published: November 2019

Abstract
Background: The efficacy and safety of lapatinib plus capecitabine (LC or LX) versus trastuzumab plus chemotherapy in patients with HER-positive metastatic breast cancer who were resistant to trastuzumab is largely unknown. Methods: we retrospectively analyzed breast cancer patients who began the treatment with regimen of lapatinib plus capecitabine (LC or LX) or trastuzumab beyond progression (TBP) at eight hospitals between May 2010 and October 2017. Results: Among 554 patients who had developed resistance to trastuzumab, the median PFS was 6.77 months in the LX group compared with 5.6 months in the TBP group (hazard ratio 0.804; 95% CI, 0.67 to 0.96; P=0.019). The central nervous system progression rate during treatment was 5.9% in LX group and 12.5% in TBP group, respectively (P=0.018). PIK3CA and TP53 are the most common mutations in resistant patients, but do not significantly differ between primary resistant cancers and secondary resistant cancers. Conclusion: The combination of lapatinib and capecitabine has shown a prolonged PFS compared with TBP in patients who had progressed on trastuzumab.
Light alcohol drinking and the risk of developing cancer, a systematic review.
Authors: Ciardiello Davide , Caprio Giuseppe Gerardo , Picascia Desiree , Dallio Marcello , Vitiello PietroPaolo , Giunta Emilio Francesco , Falco Vincenzo De , Famiglietti Vincenzo , Martinelli Erika , Gravina Antonietta , Federico Alessandro , Ciardiello Fortunato , Loguercio Carmelina 
Published: November 2019

Abstract
Background: There is strong evidence that heavy alcohol consumption (>50g per day) is a risk factor for several disease, including cancer. However, the oncogenic role of light alcohol drinking (<12,5g per day) is still controversial. The purpose of this review is to assess whether the intake of a single daily dose of alcohol could determine an increased risk of malignancy onset. Methods: A systemic PubMed research of meta-analysis studies, using the keywords “light alcohol drinking”, “light alcohol consumption” and “cancer”, was done. We established a period of 15 years, in order to select the most recent evidences; exclusively meta-analysis that specified levels of alcohol intake were included in the review. Studies focused on biochemical, molecular and genetic aspects, as well as duplicate articles were excluded. Results: We included in this review 29 large meta-analysis; light alcohol drinking was not associated with an increased risk of occurrence of most cancers, with the exception of breast, prostate cancer and, with less evidence, melanoma. Furthermore, a protective role of alcohol assumption on the development of bladder, kidney ovarian cancer and Non Hodgkin Lymphoma (NHL), has been described.Conclusions: We found that light alcohol drinking do not determine a higher risk for the insurgence of several malignancies, except for a light increase in the incidence of melanoma, breast cancer in women and prostate cancer in men.
Suppression of behavioral activity and hippocampal adrenaline caused by surgical stress in type 2 diabetes model mice
Authors: Nishimura Momoka , Nomura Yuki , Egi Moritoki , Obata Norihiko , Tsunoda Makoto , Mizobuchi Satoshi 
Published: November 2019

Abstract
Background In these days, the occurrence of neuropsychological complications during perioperative period has become the subject of discussion. Diabetes is indicated as one of the metabolic risk factors. Although the number of patients with diabetes mellitus (DM) has been increasing, the pathophysiology of postoperative neuropsychological dysfunction in DM patient is still unclear. Recently, a deficiency of neurotransmitters, such as monoamines, was reported to be associated with mental disorders. Therefore, we investigated the effects of surgical stress on behavioral activity and the hippocampal noradrenaline (NA) level in type 2 diabetes mellitus model (T2DM) mice.Methods Eighty-four 6-week-old male C57BL/6J mice were divided into four groups (control, surgery control, T2DM, and surgery T2DM groups). T2DM mice were established by feeding a high fat diet (HFD) for 8 weeks. At 14 weeks of age, fifteen mice in every group underwent a series of behavioral tests including an open field (OF) test, a novel objective recognition (NOR) test and a light-dark box (LD) test. In the surgery groups, open abdominal surgery with manipulation of intestine was performed 24 hours before the behavioral tests as a surgical stress. Hippocampal NA level was examined in six mice in each group by high-performance liquid chromatography. The data were analyzed by Mann-Whitney U test and P values less than 0.05 were considered significant.Results The T2DM group showed significantly increased explorative activity in the NOR test (P = 0.0016) and significantly increased transition in the LD test (P = 0.043) compared with those in the control group before surgery. In T2DM mice, surgical stress resulted in decreased total distance in the OF test, decreased explorative activity in the NOR test, and decreased transition in the LD test (OF: P = 0.015, NOR: P = 0.009, LD: P = 0.007) and decreased hippocampal NA (P = 0.015), although they were not revealed in control mice.Conclusion T2DM mice by feeding a HFD showed increased behavioral activities, and surgical stress in T2DM mice caused postoperative hypoactivity and reduction of hippocampal NA level.
On Orientation of Visually Impaired People in Public Space
Authors: KOMAR Beata 
Published: November 2019

Abstract
The topic of this paper focuses on orientation of visually impaired people in public space.
The purpose of the paper is to show the ways visually impaired people move and therefore, to show the barriers which hinder them to get someplace. An important element is to increase social awareness of the quality of public spaces so that they could be adapted to every user. The paper was written on the basis of literature analyses and the author’s own observation.
Health-Affirming Everyday Landscapes in Sustainable City. Theories and Tools
Authors: TROJANOWSKA Monika , SAS-BOJARSKA Aleksandra 
Published: November 2019

Abstract
As cities and urban population continue to grow, causing serious threats to public health, the development of health-affirming urban landscapes becomes even more important topic than ever before. The purpose of this paper is to answer the question which qualities of urban landscape make it the health-affirming landscape. In the first part of the paper, a concept of health-affirming landscapes and a modern approach to sustainable city design are examined. In the second part, the qualities of health-affirming urban landscape according to theories and research are discussed. The tools, which might be applied to urban design to create health-affirming landscapes are reviewed. The conclusions concern the need and possibilities to create health-affirming landscapes. The need for further research on impact of such landscapes on health and well-being of city dwellers is indicated.
Random Strength Parameters of Steel Corrugated Webs and Their Influence on the Resistance of Sin Plate Girders
Authors: BASIŃSKI Witold , KOWAL Zbigniew 
Published: November 2019

Abstract
In the study, the results of statistical investigations into random strength parameters of steel used to manufacture corrugated webs of SIN plate girders were discussed. The investigations were conducted on samples randomly collected from twenty SIN girders. The corrugated web of the girders was 2, 2.5 and 3 mm in thickness. The girders had been examined before statistical investigations were conducted. Based on the analysis, coefficients of variation of yield strength V
Re=D(R
e)/E(R
e) and partial factors of yield strength γ
m
were identified. The results were compared with factors γ
m
obtained from investigations into thin flat sheets [10]. The impact of yield strength and random cross-sectional area of folded web on the quantile of girder web resistance was shown. Taking into account random character of yield strength and web thickness offers a sensible approach to SIN girder design and computations.
Impact of the UV Lamp Power on the Formation of Swimming pool Water Treatment By-Products
Authors: KUDLEK Edyta , LEMPART Anna , DUDZIAK Mariusz , BUJAK Marta 
Published: November 2019

Abstract
The operation of swimming pools requires a constant monitoring of water quality parameters and protection of water against pathogens. This is implemented by various disinfection methods, among which the most commonly used are based on chlorine action supported by ozone or UV irradiation. The paper presents the comparison of the effectives of organic micropollutants decomposition occurring in swimming pool water during UV irradiation emitted by a 15 and 150 W UV lamp. The tests were conducted on real swimming pool water collected from a sport basin. The identification and the determination of micropollutants concentration were performed by the use of gas chromatography GC-MS (EI) preceded by solid-phase extraction SPE. It was shown that the concentration of micropollutants decreases with the increase in the irradiation time of pool water. The 150 W UV lamp allowed for an over 33% removal of micropollutants from the group of pharmaceuticals compounds (except for caffeine) and more than 76% decrease of other compounds, which belong to the group of personal care products additives, food additives and phthalates. In addition, it has been demonstrated that during the irradiation of such complex water matrixes as swimming pool water, a significant number of micropollutants degradation by-products were formed, which are not found in water before UV irradiation.
Coagulant Cost Optimization for Surface Water Coagulation Process
Authors: WOLSKA Małgorzata , MOŁCZAN Marek , SOLIPIWKO-PIEŚCIK Anna , URBAŃSKA-KOZŁOWSKA Halina 
Published: November 2019

Abstract
This paper presents the results of a study concerning application of different coagulants used in surface water coagulation process. Even though the use of this process is quite wide-spread and several different coagulants are used it would be vital to identify which coagulants are the most effective from the economical point of view. The choice of parameter, such as doses, contact time, speed of mixing are important for cost optimization while maintaining satisfactory results for WTP. Four of the most effective coagulants were taken to the second stage. Three of the four coagulants chosen for the second stage of the study were the same for both tests regardless at water quality parameters. The cost of the process depended above all on raw water contamination, and therefore the coagulant dosage.
An analysis of the results has shown that only the non pre-hydrolyzed coagulant allowed for high process effectiveness at low process costs. This coagulant was also the only one to require a pH correction after coagulation. However, this did not significantly increase the coagulation cost.