bluuebunny/crossref_metadata_2025_split huggingface dataset:
Surge Effects and Survival to Hospital Discharge in Critical Care Patients with COVID-19 During the Early Pandemic: A Cohort Study
Authors: Dale Christopher , Starcher Rachael , Chang Shu Ching , Robicsek Ari , Parsons Guilford , Goldman Jason , Vovan Andre , Hotchkin David , Gluckman Ty 
Published: November 2020

Abstract
BackgroundThe early months of the COVID-19 pandemic were fraught with much uncertainty and some resource constraint. We assessed the change in survival to hospital discharge over time for intensive care unit patients with COVID-19 during the first three months of the pandemic and the presence of any surge effects on patient outcomes.MethodsRetrospective cohort study with electronic medical record data of all patients with laboratory-confirmed COVID-19 admitted to intensive care units from February 25, 2020 to May 15, 2020 admitted to intensive care units of 26 hospitals of an integrated delivery system in the Western United States. Patient demographic, comorbidity and severity of illness were measured along with exposure to pharmacologic and medical therapies and hospital outcomes over time. Multivariable logistic regression models were constructed to assess the change in survival to hospital discharge over time during the study period.ResultsOf 620 patients with COVID-19 admitted to the study ICUs (mean age 63.5 years (SD 15.7) and 69% male), 403 (65%) survived to hospital discharge and 217 (35%) died in hospital. Survival to hospital discharge increased over the study period from 60.0% in the first two weeks of patient admission to 67.6% in the last two weeks. In a multivariable logistic regression analysis, the risk-adjusted odds of survival to hospital discharge increased over time (bi-weekly change, adjusted odds ratio [aOR] 1.22, 95%CI 1.04-1.40, P = 0.02). Additionally, an a priori-defined explanatory model showed that after adjusting for both hospital occupancy and COVID positive/PUI percent hospital capacity, and the same set of covariates, the temporal trend in risk-adjusted patient survival to hospital discharge remained the same (bi-weekly change, aOR 1.18, 95% CI 1.00 to 1.38, P = 0.04) and a greater COVID positive/PUI percentage of hospital capacity remained significantly and inversely associated with survival to hospital discharge (aOR 0.95, 95% CI 0.92 to 0.98, P < 0.01).ConclusionsDuring the the early COVID-19 pandemic, risk-adjusted survival to hospital discharge increased over time for critical care patients. This may have been partially explained by surge affects, as measured by a greater COVID positive/PUI percentage of hospital capacity.
Caregiver Burden and Emotional Wellbeing in Informal Caregivers to ICU&nbsp;Survivors: A Prospective Cohort Study
Authors: Milton Anna , Schandl Anna , Larsson Ing-Marie , Wallin Ewa , Savilampi Johanna , Meijers Katarina , Joelsson-Alm Eva , Bottai Matteo , Sackey Peter 
Published: November 2020

Abstract
BackgroundInformal caregivers to intensive care unit (ICU) survivors may develop post-intensive care syndrome family (PICS-F), including psychological problems such as depression, anxiety and post-traumatic stress (PTS). Our primary aim was to investigate associations between caregiver burden in informal caregivers cohabiting with ICU survivors and patients’ physical and psychological outcomes.MethodsWe conducted a prospective, multicentre cohort study in four ICUs in Sweden. Adults cohabiting with ICU patients included in a previous study were eligible for inclusion. Three months post-ICU, informal caregivers received questionnaires assessing perceived caregiver burden, health-related quality of life (HRQL) and symptoms of depression, anxiety and PTS. In parallel, patients reported their physical and psychological status via validated questionnaires. The primary outcome was to compare caregiver burden in informal caregivers to patients with and without adverse physical and psychological outcomes three months post-ICU. Secondary outcomes were correlations between caregiver burden and informal caregivers’ psychological status and mental HRQL.ResultsAmong 62 included informal caregivers, 55 (89%) responded to the follow-up questionnaires. Caregiver burden was higher in informal caregivers to patients with an adverse psychological or physical outcome, compared to informal caregivers to patients without an adverse outcome, caregiver burden scale score mean (±standard deviation) 52 (11) and 41 (13) respectively (p=0.003). There was strong negative correlation between caregiver burden and informal caregivers’ mental HRQL (rs -0.74, p<0.001). ConclusionInformal caregivers to ICU survivors with an adverse physical or psychological outcome experience a higher caregiver burden. A higher caregiver burden correlates with worse caregiver mental HRQL. ICU follow-up programs should consider screening and follow-up of informal caregivers for mental health problems.Trial registrationThe study was registered at clinicaltrials.gov, NCT02712541 on March 18 2016.
Pulmonary Dysfunction in Patients Recovered from COVID-19 Pneumonia: A 6-Month Follow-up Study
Authors: Meng Xianglin , Kang Kai , Gao Yang , Fei Dongsheng , Yang Wei , Yang Songlin , Zhang Meiling , Lu Baitao , Meng Shishuai , Li Xianyong , Liu Wen , Jin Songgen , Kong Weilan , Chong Yang , Liu Yan , Wu Haolan , Sun Chongyang , Guan Xue , Wang Changsong , Zhao Mingyan , Yu Kaijiang 
Published: November 2020

Abstract
Objectives: This study investigates the clinical features and pulmonary functions of COVID-19 pneumonia survivors at 3 or 6 months after diagnosis in the Heilongjiang Province, China.Methods: Forty-six patients with COVID-19 pneumonia diagnosed since February 2020 were enrolled in this study for follow-up in July 2020. These patients were categorized into three groups: Group A (n=24) and Group B (n=11) who were diagnosed with moderate or severe pneumonia and followed up at three months after diagnosis; Group C (n=11) who were diagnosed with severe pneumonia and followed up at six months after diagnosis. Data on pulmonary function, arterial blood gas analysis, chest CT, blood test, antibody test, and health-related quality of life during hospitalization and at the follow-up visits were collected and analyzed. Results: Abnormal PO2 (A-a) was more prevalent in severe cases (Group B and C) than in moderate cases (Group A). Pulmonary dysfunction was common in this cohort. Abnormal CT scores of severe cases (Group B and C) were significantly higher than that of moderate cases (Group A). During the follow-up, lung abnormalities gradually resolved in the first 3 months (Group A and B), however, further resolution was not significant from 3 months to 6 months (Group B and C). Conclusion: Although pulmonary interstitial changes due to COVID-19 pneumonia gradually reverse over time, pulmonary dysfunction is common and appears to persist at least up to 6 months in patients recovered from COVID-19 pneumonia.
Abdominal Tuberculosis With Pancreatic Head Involvement Mimicking Pancreatic Malignancy in a Young Man: A Case Report
Authors: Wang Lie-zhi , Jiang Hao , Jin Chong , Wen Yu , Zou Heng , Wang Kunpeng 
Published: November 2020

Abstract
Background：Pancreatic tuberculosis is a rare disease, even in immuocompentent hosts. Abdominal tuberculosis involving the pancreatic head and peripancreatic areas may simulate pancreatic head carcinoma.Case presentation: We herein present the case of a 32-year-old man who was admitted to our hospital for intermittent epigastric pain and weight loss. Computed tomography scan and magnetic resonance imaging revealed a mass in the head of the pancreas.The lesion was initially diagnosed as pancreatic head carcinoma on abdominal imaging. Laparotomy confirmed the diagnosis of pancreatic tuberculosis, while he test for acid-fast bacilli was negative before operation and the patient fully recovered after six month of standard anti-tuberculosis treatment.Conclusions: The present case is reported to emphasize the importance of including pancreatic tuberculosis in the differential diagnosis of pancreatic lesions, under the premise of safety, we recommend endoscopic ultrasound-guided fine needle aspiration biopsy for diagnosis.
Efficacy of upfront hepatectomy without neoadjuvant chemotherapy for resectable colorectal liver metastasis
Authors: Ono Kosuke , Abe Tomoyuki , Ohshita Akihiko , Sumi Yusuke , Yano Takuya , Okuda Hiroshi , Kurayoshi Manabu , Kobayashi Tsuyoshi , Ohdan Hideki , Noriyuki Toshio , Nakahara Masahiro 
Published: November 2020

Abstract
Background
This study evaluated the impact of neoadjuvant chemotherapy on the prognosis of patients with resectable colorectal liver metastasis and assessed the usefulness of Beppu’s nomogram for predicting prognosis.
Methods
This retrospective study identified 88 consecutive inpatients who underwent primary hepatic resection for colorectal liver metastasis; 58 received neoadjuvant chemotherapy and 30 underwent upfront surgery. Factors associated with recurrence-free survival were identified via univariate and multivariate analysis. Propensity score analysis using inverse probability of treatment weighting was also performed.
Results
On univariate analysis, poor recurrence-free survival was associated with multiple tumors, advanced primary tumor stage, vascular invasion by the primary tumor, a Beppu score ≥ 6, and neoadjuvant chemotherapy. On multivariate analysis, a Beppu score ≥ 6 and neoadjuvant chemotherapy were independent risk factors for recurrence. Neoadjuvant chemotherapy recipients had a higher incidence of lymph node metastasis and vascular invasion than non-recipients. Propensity score analysis revealed no significant difference in the recurrence-free survival rate between these groups.
Conclusions
Our results show the usefulness of Beppu scores for predicting the prognosis of patients with colorectal liver metastasis, but do not support the use of neoadjuvant chemotherapy in these patients.
Correlation and Mechanism Study of Multivoxel 1H-MRS&nbsp;Parameters, Cho / Cr and CHKA
Authors: Yue Fangqian , Zou YouRui , Sun ShengYu , Ma Hui , Wang Zhe , Gao Xinyi , Huang Ling 
Published: November 2020

Abstract
Background Glioma is a common malignant brain tumor with strong invasiveness and poor prognosis. in order to improve the survival rate of patients, there is an urgent need to find the targeted molecular mechanism affecting its invasion and development. Methods In this study, the differential regions of Cho/Cr metabolism of tumor cells were identified by multi-voxel 1H-MRS and the tumor tissues of Cho/Cr high metabolism group and Cho/Cr low metabolism group were accurately obtained by combining neuronavigation system. The expression of CHKA in different Cho/Cr metabolic groups was verified by Real-timePCR and Western-blot. Then we used CHKA inhibitor MN58b and short hairpin RNA to study the role of CHKA in the proliferation and invasion of U251 glioma cells, and the relationship between CHKA and EMT in nude mice.Results We found that the expression of CHKA in Cho/Cr high metabolism group was significantly higher than that in Cho/Cr low metabolism group. Decreased CHKA activity can inhibit the proliferation and invasion of glioma cells and slow down the process of EMT.Conclusion CHKA may be an oncogenic gene, which affects the invasion and development of glioma cells by regulating the process of tumor EMT, and is expected to become a molecular target for glioma therapy, and multi-voxel 1H-MRS parameter Cho/Cr may become an important clinical index for detecting CHKA expression.
A Well-differentiated Liposarcoma of the Prevertebral Space: A Case Report
Authors: Yang Tianming , Xiao Lin , Ren Huijun 
Published: November 2020

Abstract
Background: Liposarcoma is common in adults. However, its occurrence in the retropharyngeal space is extremely rare, and no cases of liposarcoma in the prevertebral space have been reported before.Case presentation: A 78-year-old man presented to the Otolaryngology Department with a 1-year history of progressive dysphagia and a 1-month history of dyspnea and dysphonia. A computed tomography scan of his neck demonstrated a large retropharyngeal mass. Magnetic resonance imaging was performed to further characterize this mass. Based on the above examinations, retropharyngeal liposarcoma was first made. The patient underwent transoral surgical excision with the help of nasal endoscope. During surgery, the tumor was found to lie in the prevertebral space. Combine with histological examination, the diagnosis was modified to liposarcoma of the prevertebral space. Conclusions: It’s difficult to distinguish the enormous retropharyngeal tumors and prevertebral tumors using imaging. Complete excision via transoral approach maybe enough for these tumors.
Mechanism of Hydrogen Gas Promoted Apoptosis of Lung Adenocarcinoma A549 cells through XIAP and BIRC3
Authors: Zhang Yu , Chen Gang , Yan Feng Zhen , Wang Fei Li , Wang Chang Dong 
Published: November 2020

Abstract
Background/AIMLung cancer is the most common reason of cancer-related death in worldwide. Hydrogen gas has been found to have effects on a variety of diseases. At present, it is not reported that the effect of hydrogen gas on lung cancer domestic and overseas. Therefore, we designed this experiment to test the differences in the expression of XIAP, BIRC3 and BAX In vivo and in vitro. Materials and methodsA549 cells in logarithmic phase were treated by 20%, 40%, 60% hydrogen gas respectively. Then the apoptosis of different groups were detected by Flow cytometry. We identify the differential expressed genes(DEGs) by transcriptional. The protein expression of XIAP, BIRC3 and BAX were detected by western blot and immunohistochemistry. ResultThe results demonstrated that hydrogen gas can significantly induce apoptosis compared with the control group. The expression of XIAP and BIRC3 were downregulated in hydrogen group. Conclusion﻿Hydrogen gas may promote the apoptosis of lung cancer A549 cells by reducing the expression of XIAP and BIRC3 protein.
Bioinformatic Analysis: Screening and Identification of Differentially Expressed Genes&nbsp;Modified by m6A&nbsp;in Ovarian Cancer
Authors: Liu Huidong , Zhang Wen-wen , Lou Ge 
Published: November 2020

Abstract
Background: N6-methyladenosine(m6A) is one of the most common RNA modifications that occurs at the nitrogen-6 position of adenine. Emerging evidence has revealed that regulatory functions of m6A play an essential role in the development of cancer. However the study of m6A in ovarian cancer(OC) is still in our infancy. In this work ,we aimed to identify and analysis the differentially expressed genes(DEGs） modified by m6A which can provide new therapeutic targets and key biomarkers in OC.Methods: We downloaded Microarray datasets GSE146553 and GSE124766 from Gene Expression Omnibus (GEO) database. The differentially expressed genes (DEGs) were identified by GEO2R analysis tools. Subsequently, The DAVID database was used to construct Enrichment analysis of GO and KEGG pathways. Next, the DEGs modified by m6A were identified by m6AVar database. Finally, the functional analysis and clinical sample validation of these genes were verified by ONCOMINE, GEPIA, cBioPortal online platform and Kaplan-Meier Plotter.Results:152 DEGs were selected ,and the DEGs were mainly enriched in extracellular exosome, spindle microtubule, response to hypoxia and cell cycle .And we identified 15 DEGs which were modified by m6A:MAPK10、MXRA5、CHD7、MECOM、SCN7A、GREB、PRUNE2、MX2、TOP2A、JAM2、DST、LAPTM5、CDKN2A、GATM and ANGPTL1. After statistical analysis, two DEGs (SCN7A and GAMT) were selected for detailed study. We revealed that SCN7A and GAMT were expressed at a low level in OC. Afterwards, Survival analysis showed that SCN7A and GAMT expression were correlated with OC overall survival. And the expression of SCN7A and GAMT mRNA decreasing in different TNM stages. Finally, we presumed that the modification of m6A spongs GAMT via EIF4A3 or FUS to participate in the occcurrence and the development of OC.Conclusion: Altogether, the current study identified and analysised the DEGs modified by m6A in OC. It will help us to investigate the underlying mechanism and progression of OC. In addition, it can provide new diagnostic markers and potential therapeutic targets in OC.
The Short-Term Efficacy of Novel No-Touch Combined Directional Perfusion Radiofrequency Ablation in the Treatment of Small Hepatocellular Carcinoma With Cirrhosis.
Authors: Qu Chengming , Li Xin-Qian , Xia Feng , Feng Kai , Ma Kuansheng 
Published: November 2020

Abstract
BackgroundNo-touch combined directed perfusion radiofrequency ablation (NTDP-RFA) is a new technique for the treatment of hepatocellular carcinoma (HCC). The purpose of this study was to evaluate the short-term efficacy of this new technique for the treatment of small HCC with cirrhosis.MethodsFrom January 2017 to March 2018, 56 consecutive patients treated with NTDP-RFA at our center were enrolled in this retrospective study. All NTDP-RFA procedures involved the use of internally cooled wet electrodes with a directional injection function, which can perform both intraelectrode cooling and extraelectrode saline perfusion. Survival curves were analyzed using Kaplan-Meier methods, and Cox proportional hazards regression analyses were used to assess predictors of tumor progression and overall survival. Operative characteristics and complications were also assessed.ResultsNo technical failure occurred, and the complete ablation rate after single NTDP-RFA treatment was 98.2%. The median ablation time was only 8 (6-8) min. Only 5 patients (8.9%) experienced mild complications postoperation, and the median hospital stay was only 4 (3-5) days. In the 18 patients (32.1%) with poor liver function reserve (indocyanine green retention rate at 15 min > 15%), their liver function returned to normal on the third day after the postoperation. The 1-year and 2-year local and distant progression rates were 1.7%, 7.1%, 3.5% and 10.7%, respectively.ConclusionsNTDP-RFA in the treatment of small HCC with cirrhosis has a low incidence of complications and provides a high survival rate without local tumor progression. Further prospective randomized controlled studies are needed to investigate the long-term results.
Associations Between Dynamic Contrast Enhanced Magnetic Resonance Imaging and Clinically Relevant Histopathological Features in Breast Cancer. A Multicenter Analysis
Authors: Surov Alexey , Pech Maciej , Kim Jin You , Aiello Marco , Huang Wei , Yankeelov Thomas E. , Wienke Andreas 
Published: November 2020

Abstract
Background: To provide evident data regarding relationships between quantitative dynamic contrast enhanced magnetic resonance imaging (DCE MRI) and prognostic factors in breast cancer (BC).Methods: Data from 4 centers (200 female patients, mean age, 51.2 ± 11.5 years) were acquired. The following data were collected: histopathological diagnosis, tumor grade, stage, hormone receptor status, KI 67, and DCE MRI values including Ktrans (volume transfer constant), Ve (volume of the extravascular extracellular leakage space (EES) and Kep (diffusion of contrast medium from the EES back to the plasma). DCE MRI values between different groups were compared using the Mann–Whitney U test and by the Kruskal-Wallis H test. The association between DCE MRI and Ki 67 values was calculated by Spearman’s rank correlation coefficient. Results: DCE MRI values of different tumor subtypes overlapped significantly. There were no statistically significant differences of DCE MRI values between different tumor grades. All DCE MRI parameters correlated with KI 67: Ktrans, r = 0.44, p=0.0001; Ve, r = 0.34, p=0.0001; Kep, r = 0.28, p=0.002. ROC analysis identified a Ktrans threshold of 0.3 min-1 for discrimination of tumors with low KI 67 expression (<25%) and high KI 67 expression (≥25%): sensitivity, 75.5%, specificity, 73.0%, accuracy, 74.0%, AUC, 0.78. DCE MRI values overlapped between tumors with different T and N stages.Conclusion: Ktrans, Kep, and Ve cannot be used as reliable a surrogate marker for hormone receptor status, tumor stage and grade in BC. Ktrans may discriminate lesions with high and lower proliferation activity.
The Expression of CLDN11 in Invasive Ductal and Invasive Lobular Breast Cancer and Multivariate Prognostic Analysis
Authors: Cui Yu , Du Qiupei , Zhang Zhanhong , Feng Haojie , Hu Wang , Jin Guoquan , Fan Haining 
Published: November 2020

Abstract
Background: Breast cancer (BRCA) remains the most common malignancy among women worldwide. Invasive ductal (IDC) and invasive lobular breast cancer (ILC) are the most frequent histological subtypes. Many genetic heterogeneities are related to the carcinogenesis of breast cancer, but exact reasons are still unclear. The study aimed to perform a clinical multivariate analysis of CLDN11 in ILC and IDC. Methods: Datasets analyzed CLDN11 expression in IDC and ILC from The Cancer Genome Atlas (TCGA) database, and the multiple analysis of stratification in terms of clinical pathologic features and CLDN11 was using the Chi-square test. Results: CLDN11 expression was significantly decreased in IDC and ILC data of GEO and TCGA compared to the normal breast tissues. Besides, CLDN11 is negatively correlated with pro-oncogene IKBKE expression and positively correlated with tumor suppressor ETS1 expression in IDC and ILC samples. Multiple factors, including age, clinical stage, subtype, and mutation status, indicated that the expression of CLDN11 is positively associated with the progression of IDC and ILC.Conclusions: CLDN11 acted as a tumor suppressor in IDC and ILC patients and serves as a drug-gable target against IDC and ILC.
Nephron-sparing Surgery Versus Radical Nephroureterectomy in the Treatment of Organ-localized Upper Urinary Tract Urothelial Carcinoma: A Population-based Study of 1581 Patients
Authors: Qiu Jianhui , Liu Hua , Wang Zixin , Zhou Jingcheng , Gong Yanqing , Cai Lin , Gong Kan 
Published: November 2020

Abstract
Purpose: Nephron-sparing surgeries (NSS) have been widely used for patients with low-risk upper tract urothelial carcinoma (UTUC). We compared the prognosis of patients with organ-localized UTUC after NSS and radical nephroureterectomy (RN) through a population-based study and a propensity score matching (PSM) method. We further developed prognostic models for these patients using the Cox analysis and the competing-risk models.Materials and methods: The follow-up dataset of 1580 patients with organ-localized UTUC were screened out from the Surveillance, Epidemiology, and End Results (SEER) registry. A PSM process with a caliper width of 0.05 was performed to match the patients accepting NSS with those accepting RN. The Cox analysis was used for overall survival (OS) and competing-risks models based on Fine-Gray regression for cancer-specific survival (CSS). A forward stepwise selection process was applied to select independent predictors for OS and CSS. After selecting the predictors, two nomograms were developed based on the Cox analysis and the competing-risk model and internal validations were performed using 1000 bootstrap resamples.Results: Before PSM, RN was associated with better OS compared with NSS in the overall cohort (5-year OS rate: 65.1% vs. 55.6%, p<0.001) and the patients with renal pelvic lesions (5-year OS rate: 68.6% vs. 52.8%, p=0.001). Baseline variables were well balanced using the PSM method. After PSM, there was no significant difference in OS between the RN and NSS groups. Competing-risks models revealed that NSS was associated with poorer CSS compared with RN in the post-PSM cohort (n=766; subdistribution hazard ratio/ SHR=1.39, 95% confidence interval/ CI 1.03-1.89, p=0.033) and in patients with ureteral lesions from the post-PSM cohort. Four independent predictors, which were age at diagnosis, AJCC T stage, histologic grade, and surgery methods, were selected out for OS and CSS through forward stepwise selection methods. Two nomograms for OS and CSS were respectively developed. Conclusions: Patients accepting RN had a comparative or better outcome in each sample group. NSS achieved a similar prognosis for selective patients with organ-localized UTUC. Two nomograms were developed to predict OS and CSS with C-indexes of 0.672 and 0.643.