bluuebunny/crossref_metadata_2025_split huggingface dataset:
Increasing of malignancy of breast cancer cells after cryopreservation: molecular detection and activation of angiogenesis after CAM-Xenotransplantation.
Authors: Du Xin , Todorov P. , Isachenko Evgenia , Rahimi G , Mallmann P. , Meng Yuanguang , Isachenko Vladimir 
Published: July 2020

Abstract
Background: Ovarian tissue cryopreservation has a wide range of cancerous indications. Avoiding relapse becomes a specific concern that clinicians frequently encounter. The data about the comparative viability of cancer cells after cryopreservation are limited. This study aimed to evaluate the effect of cryopreservation on breast cancer cells. Methods: We used in-vitro cultured ZR-75-1 and MDA-MB-231 cell lines. Cell samples of each lineage were distributed into the non-intervened and cryopreserved groups. The cryopreservation procedures comprised programmed slow freezing followed by thawing at 100°C, 60 s. Biological phenotypes and the related protein markers were compared between the two groups. The EVOS FL Auto 2 Cell Image System was used to monitor cell morphology. Cell proliferation, motility, and penetration were characterized by CCK-8, wound-healing, and transmembrane assay, respectively. The expression of Ki-67, P53, GATA-3, E-cadherin, Vimentin, and F-Actin was captured by immunofluorescent staining and western blotting as the proxy measurements of the related properties. The chorioallantoic membrane (CAM) xenotransplantation was conducted to explore angiogenesis induced by cancer cells. Results: After 5-days in vitro culture, the cell concentration of cryopreserved and non-intervened groups was 15.7×104 vs. 14.4×104cells/ml, (ZR-75-1, p>0.05), and 25.1 ×104 vs. 26.6×104 cells/ml (MDA-MB-231, p>0.05). Some cryopreserved ZR-75-1 cells presented spindle shape with filopodia and lamellipodia and dissociated from the cell cluster after cryopreservation. Both cell lines demonstrated increased cell migrating capability and invasion after cryopreservation. The expression of Ki-67 and P53 did not differ between the cryopreserved and control groups. E-cadherin and GATA3 expression downregulated in the cryopreserved ZR-75-1 cells. Vimentin and F-actin exhibited upregulated level in cryopreserved ZR-75-1 and MDA-MB-231 cells. The cryopreserved MDA-MB-231 cells induced significant angiogenesis around the grafts on CAM with the vascular density 0.313±0.03 and 0.342±0.04, compared with that of fresh cells of 0.238±0.05 and 0.244±0.03, p<0.0001.﻿Conclusions: Cryopreservation promotes breast cancer cells in terms of epithelial-mesenchymal transition and angiogenesis induction, thus increasing metastasis risk.
Factors affecting adolescents’ participation in randomized controlled trials evaluating the effectiveness of healthcare interventions: the case of the STEPSTONES project
Authors: Saarijärvi Markus , Wallin Lars , Moons Philip , Gyllensten Hanna , Bratt Ewa-Lena 
Published: July 2020

Abstract
Background Recruitment of adolescents to intervention studies is a known challenge. For randomized controlled trials (RCT) to be generalizable, reach must be assessed, which means ascertaining how many of the intended population actually participated in the trial. The aim of this study was to evaluate the reach and representativeness of an RCT evaluating the effectiveness of a complex intervention for adolescents with chronic conditions.Methods A mixed methods sequential explanatory design was employed. Firstly, quantitative cross-sectional data from the RCT, patient registries and medical records were collected and analysed regarding baseline differences between participants and non-participants in the trial. Secondly, qualitative data on their reasons for participating or not were collected and analysed with content analysis to explain the quantitative findings.Results Participants showed larger differences in effect sizes and a significantly more complex chronic condition than non-participants. No other statistically significant differences were reported, and effect sizes were negligible. Reasons for declining or accepting participation were categorized into three main categories: altruistic reasons, personal reasons and external reasons and factors. Conclusions Integration of quantitative and qualitative findings showed that participation in the RCT was affected by disease complexity, the perceived need to give back to healthcare and research and the adolescents’ willingness to engage in their illness. To empower adolescents with chronic conditions and motivate them to participate in research, future intervention studies should consider developing tailored recruitment strategies and communications with sub-groups that are harder to reach.
Coeruptive and posteruptive crustal deformation associated with the 2018 Kusatsu-Shirane phreatic eruption based on PALSAR-2 time-series analysis
Authors: Himematsu Yuji , Ozawa Taku , Aoki Yosuke 
Published: July 2020

Abstract
Coeruptive deformation helps to interpret physical processes associated with volcanic eruptions. Because phreatic eruptions cause small, localized coeruptive deformation, we sometimes fail to identify plausible deformation signals. Satellite synthetic aperture radar (SAR) data allow us to identify extensive deformation fields with high spatial resolutions. Herein, we report coeruptive crustal deformation associated with the 2018 Kusatsu-Shirane phreatic eruption detected by time series analyses of L-band satellite SAR (ALOS-2/PALSAR-2) data. Cumulative deformation maps derived from SAR time series analyses show that subsidence and eastward displacement dominate the southwestern side of an eruptive crater with a spatial extent of approximately 2 km in diameter. Although we were unable to identify any significant deformation signals before the 2018 eruption, posteruptive deformation on the southwestern side of the crater has been ongoing until the end of 2019. This prolonged deformation implies the progression of posteruptive physical processes within a confined hydrothermal system, such as volcanic fluid discharge, similar to the processes observed during the 2014 Ontake eruption. Although accumulated snow and dense vegetation hinder the detection of deformation signals on Kusatsu-Shirane volcano using conventional InSAR data, L-band SAR with various temporal baselines allowed us to successfully extract both coeruptive and posteruptive deformation signals. The extracted cumulative deformation is well explained by a combination of normal faulting with a left-lateral slip component along a southwest-dipping fault plane and an isotropic deflation. Based on the geological background in which the shallow hydrothermal system develops across Kusatsu-Shirane volcano, the inferred dislocation plane can be considered as a degassing pathway from the shallow hydrothermal system to the surface due to the phreatic eruption. We reconfirmed that SAR data is a robust tool for detecting coeruptive and posteruptive deformations, which are helpful for understanding shallow physical processes associated with phreatic eruptions at active volcanoes.
The effect of different levels of prostate-specific antigen and Prostate Imaging Report and Data System scores on logistic regression model to predict clinically significant prostate cancer: a single center retrospective study
Authors: Zhang Loudong , Zhu Hua , Gu Donghua , Pan Xiaodong , zheng bing 
Published: July 2020

Abstract
Background

At present, there are various clinical regression models for predicting prostate cancer. But what about the diagnostic effectiveness of these models in different parameter ranges, and are the models applicable to everyone? This study aimed to study the influence of different levels of prostate-specific antigen (PSA) and Prostate Imaging Report and Data System version 2 (PI-RADS v2) scores on the regression model to predict clinically significant prostate cancer (csPCa).

Methods

This retrospective study screened 251 patients from our hospital, who were divided into different groups. The regression model was established for each group to predict csPCa, and the effects of PSA and PI-RADS scores on each model were analyzed through the diagnostic effects of the model.

Results

In patients with lower PSA scores, although the model was less sensitive than PSA, the AUC of the model was much greater. With the rise of PSA, the sensitivity of the model surpassed that of PSA, while the specificity became the opposite, and the AUC gap also gradually decreased. In the group with low PI-RADS score, the sensitivity and specificity of PI-RADS were lower than the model, and the gap was larger. Although the gap between the two gradually decreased with the increase of PI-RADS, the diagnostic efficiency of the model was still slightly larger than that of pure PI-RADS.

Conclusion

As the PSA and PI-RADS v2 scores increase, the diagnostic advantages of the regression model will gradually decrease. However, for patients with low levels of PSA and PI-RADS scores,the regression model is less affected by PSA and PI-RADS, and can better utilize its clinical diagnostic advantages.
Investigating occupational stress of disaster and investigating occupational stress of disaster and emergency medical management center 115 and the role of demographic variables
Authors: lebni Javad Yoosefi , Gheshlaghi Parvin Abdi , Toghroli Razie , Garosi Vahid Hatami , Abbas Jaffar , Kalhori Reza Pourmirza , Salahshoor Mohammad Reza , Kianipour Neda , Tharwani Munira , ziapour arash 
Published: July 2020

Abstract
Background: The first and one of the most important chains of providing care to patients is pre-hospital emergency medical services. Personnel employed in this sector are at risk of occupational stress due to the nature of their job which can affect their health and quality of services provided to patients. Therefore, the present study was conducted to investigate the occupational stress of the personnel of disaster and emergency medical management center 115 and the role of demographic variables in 2019.Methods: This is a descriptive-analytical study. 200 medical emergency personnel of Kermanshah province were selected through stratified sampling and according to inclusion criteria. A two-part questionnaire including demographic information and HSE standard questionnaire were used for collecting data. Finally, descriptive and inferential statistics (t-test and one-way ANOVA) were used for data analysis. Significance level was considered P<0.05. Results: The mean score of total occupational stress was 3.41±0.26. The highest and the lowest stress levels related to the role dimensions was calculated (4.34±0.35) and changes (2.72±0.86). There was a significant relationship between stress level with age, marital status, educational level, type of base of work place and hours of work per month, while there was no relationship between type of employment and work experience with stress level. Conclusion: Emergency medical personnel experience a high level of occupational stress. Senior managers can use the results of similar studies to think measures to reduce the experience of employees' stress.
Construct Validity of the Suboptimal Health Status Questionnaire-25 in a Ghanaian Population
Authors: Adua Eric , Afrifa-Yamoah Ebenezer , Frimpong Kwasi , Adama Esther , Karthigesu Shantha P. , Anto Enoch Odame , Aboagye Emmanuel , Yan Yuxiang , Wang Youxin , Tan Xuerui , Wang Wei 
Published: July 2020

Abstract
Background: The Suboptimal Health Status Questionnaire-25 (SHS-Q-25) developed to measure suboptimal health status has been used worldwide, but its construct validity has only been tested in the Chinese population. In this article, we investigate aspects of the construct validity of the SHS-Q-25 to determine the interactions between SHS subscales in a Ghanaian population.Methods: The study involved healthy Ghanaian participants (n = 263; aged 20-80 years; 63% female), who responded to the SHSQ-25. In an exploratory factor and parallel analysis, the study extracted a new domain structure and compared to the established five-domain structure of SHSQ-25. A confirmatory factor analysis (CFA) was conducted and the fit of the model further discussed. Invariance analysis was carried out to establish the consistency of the instrument across multi-groups. Results: The extracted domains were reliable with Cronbach’s B of 0.861, 0.821 and 0.853 respectively, for fatigue, immune-cardiovascular and cognitive, confirming the construct validity of the SHSQ-25 instrument. The CFA revealed that the model fit indices were excellent a The fit indices for the three-domain model were statistically superior to the five-domain model. There were, however, issues of insufficient discriminant validity as some average variance extracts (AVE) were smaller than the corresponding maximum shared variance (MSV). The three-domain model was invariant for all constrained aspects of the structural model across age, which is an important risk factor for most chronic diseases.Conclusion: The validity tests provide evidence to endorse the credibility of the tool and suggest that the SHS-Q25 can measure SHS in a Ghanaian population. It can be recommended as a screening tool to early detect possible for chronic diseases especially in developing countries where the access to facilities is diminished.
Neoadjuvant concurrent chemoradiotherapy followed by transanal total mesorectal excision assisted by single-port laparoscopic surgery for low-lying rectal adenocarcinoma: a single center study
Authors: Lu Yen-Jung , Chen Chien-Hsin , Lin En-Kwang , Wu Szu-Yuan 
Published: July 2020

Abstract
Purpose: To assess the feasibility and short-term outcomes of neoadjuvant chemoradiotherapy (CCRT) followed by transanal total mesorectal excision assisted by single-port laparoscopic surgery (TaTME-SPLS) for low-lying rectal adenocarcinoma.Methods and materials: A total of 23 patients with clinical stage II-III low-lying (from anal verge 0-8 cm) rectal adenocarcinoma who underwent neoadjuvant CCRT followed by TaTME-SPLS consecutively from December 2015 to December 2018, were enrolled into our study. Chi-squared testing and Student’s t testing were used to make parametric comparisons, and Fisher’s exact test or the Mann–Whitney U-test were used to make nonparametric comparisons.Results: Conversion rate in patients who underwent neoadjuvant CCRT followed by TaTME-SPLS was only 4%. The mean operation time was 366 minutes and the inter-sphincter resection (ISR) was done for 14 patients (60%). The mean number of lymph nodes harvested were 15. There was no surgical mortality, but the 30-day morbidity rate was 21% (5 patients were Clavien-Dindo I-II). Pathological complete response was 21.74% with 100% organ preservation and 100% clear distal margin after neoadjuvant CCRT followed by TaTME-SPLS. Conclusion: TaTME-SPLS would be highly successful in lymph node negative and low T stage of low-lying rectal cancer patients who had pathological complete remission or high percentage of partial remission after neoadjuvant CCRT.
Impact of Inferior Mesenteric Artery Lymph Node Metastasis on the Prognosis of Patients With Left-sided Colorectal Cancer: A Case Control Study
Authors: Takabatake Kazuya , Arita Tomohiro , Nakanishi masayoshi , Kuriu Yoshiaki , Murayama Yasutoshi , Shimizu Hiroki , Kudo Michihiro , Kiuchi Jun , Kosuga Toshiyuki , Konishi Hirotaka , Morimura Ryo , Shiozaki Atsushi , Ikoma Hisashi , Kubota Takeshi , Fujiwara Hitoshi , Okamoto Kazuma , Otsuji Eigo 
Published: July 2020

Abstract
Background:

The clinical significance of metastasis in inferior mesenteric artery (IMA) lymph node in patients with left-sided colorectal cancer (LCRC) is unclear. The aim of this study was to investigate the impact of IMA lymph node metastasis (IMA-LN (+)) on the prognosis of patients with LCRC.
Methods:

A total of 292 patients with stage III LCRC and 111 patients with stage IV LCRC who underwent radical resection of the primary tumor between 2005 and 2016 were included. The clinicopathological features and prognosis, which were retrospectively obtained from medical records, were compared regarding IMA-LN (+).
Results:

IMA-LN (+) was observed in 10 patients with stage III LCRC (2.3%). Moreover. ≥4 metastatic lymph nodes (p = 0.001) and poorly differentiated type (p = 0.049) were more frequently observed in patients with IMA-LN (+) than in patients without IMA lymph node metastasis (IMA-LN (-)) in stage III; IMA-LN (+) patients had significantly worse overall survival (OS) than IMA-LN (-) patients in stage III (p = 0.015). Conversely, there was no significant difference between the OS of stage III IMA-LN (+) and stage IV patients (p = 0.192). Likewise, there was no significant difference between the OS of stage III IMA-LN (+) and stage IV patients with distant metastatic lymph nodes only (n = 12) (p = 0.294).
Conclusion:

The prognosis of IMA-LN (+) patients was worse than that of IMA-LN (-) patients in stage III LCRC; moreover, it was similar to that of patients with stage IV LCRC.
Effect of Poor Glycemic Control Incognitive Performance Inthe Elderly With Type 2 Diabetes Mellitus: The Mexican Health and Aging Study
Authors: Mimenza-Aguilar Alberto J , Jimenez-Castillo Gilberto A , Yeverino-Castro Sara G , Barragán-Berlanga Abel , Perez-Zepeda Mario U , Avila-Funes J Alberto , Aguilar-Navarro Sara 
Published: July 2020

Abstract
Background
Cognitive impairment is twice more frequent in elderly with type 2 diabetes mellitus (DM). This study was conducted to determine the association between glycemic control and cognitive performance among community-dwelling elderly persons in Mexico.
Methods
Cross-sectional study conducted in individuals aged 60 years or elderly participating in the 2012 Mexican Health and Aging Study. Type 2 DM participants were classified in 3 groups according to their glycated hemoglobin levels (HbA1c): <7% (intensive control), 7-7.9% (standard control) or ≥ 8% (poor control), and cognitive performance: low (CCCE ≤ 44 points), intermediate (44.1-59.52 points), or high (≥ 59.53 points). Multinomial logistic regression models were constructed to determine this association.
Results
Out of 946 subjects, 216 were selected. Subjects in the low cognitive performance group were older (69.7 ± 6.6 vs 65.86 ± 5.18 years, p < .001) and had a lower educational level (2.5 ± 2.6 vs 7.44 ± 4.15 years, p < .000) when compared to the high cognitive performance participants. HbA1c ≥8% was associated with having low (OR 3.17, 95% CI 1.17–8.60, p = .024), and intermediate (OR 3.23, 95% CI 1.27–8.20, p = .014) cognitive performance; this trend was not found for HbA1c 7.0-7.9% group.
Conclusions
Glycemic control with a HbA1c ≥ 8% was associated with worse cognitive performance.
Radical Surgery of Synchronous Oligometastatic Liver Lesions of the Ductal Adenocarcinoma of the Pancreas Results in Prolonged Survival
Authors: Safi Sami Alexander , Rehders Alexander , Haeberle Lena , Keitel Verena , Fluegen Georg , Krieg Andreas , Knoefel Wolfram Trudo , Lehwald Nadja 
Published: July 2020

Abstract
Background

The ductal adenocarcinoma of the pancreas (PDAC) remains one of the most lethal malignancies. The role of surgery for circumscribed synchronous hepatic lesions of PDAC remains controversial. Thus, the aim of our study was to compare survival outcome (OS) after surgery of patients with hepatic metastases (M1surg) to patients with only localized disease.
Methods

Correlation analysis of clinicopathological data and OS after resection of M1surg patients and patients with localized PDACs (M0) was performed. Patients were included for survival analysis only if a complete staging including perineural, venous and lymphatic invasion was available.
Results

Out of the study collective, 35 patients received extended surgery (M1surg), whereas 202 patients received standardized surgery for localized disease (M0). Clinicopathological variables were homogenously distributed between M1surg and M0. The length of hospitalization and morbidity was similar in both groups. FOLFIRNOX as an adjuvant treatment regime was administered in ~ 23% and ~ 8% of M1surg and M0 patients, respectively. In a multivariate analysis, there was no difference in overall survival between both groups. Only the resection status (R1 vs R0) and venous invasion (V1) were identified as independent prognostic factors.
Conclusion

This is the first study demonstrating a survival benefit after extended surgery for synchronously hepatic-metastasized PDACs. We found no difference in survival outcome of metastasized patients when compared to patients with localized disease. FOLFIRINOX as an adjuvant treatment regime for resected M1surg presumably is worthwhile. Larger multicenter studies are still needed to validate our results.
Phytochemical Screening, Antioxidant, Antibacterial, Antifungal Potential and in vivo Acute Toxicity of the Crude Extract of Cordia Rothii and Viola Serpens
Authors: Zubair Hafiz Muhammad , Naveed Muhammad , Mikrani Reyaj , Hassan Najm ul , Taleb  , Ibrahim Muhammad , Anjum Irfan , Baig Mirza Muhammad Fran Ashraf , Jabeen Qaiser 
Published: July 2020

Abstract
Background: Plants contain numerous bioactive compounds with valuable pharmacological activities and can be used as a novel therapeutic agent. Methods: The objective of the current study was to evaluate phytochemical screening, antioxidant, antibacterial, antifungal, and acute toxicity capacity of Cordia rothii (CR) and Viola serpens (VS) plant extract. Results: The phytochemical screening revealed the presence of saponins, tannins, anthraquinones, and flavonoids contents. Antioxidant activity of both extracts was measured by total phenolic, total flavonoid content, DPPH, and reducing power capacity. IC50 value of CR and VS extract was calculated as 0.039 ± 0.05, 0.05 ± 0.07 mg/ml respectively. Both extract have antibacterial activity against S. aureus and S. epidermidis, whereas VS is inactive against E. coli. Antifungal activity was not found in both CR and VS extract. The LD50 of the test substance was also estimated to be over 10 g/kg body weight. Conclusions: The conclusion indicates that folkloric uses of both plants in traditional settings, the extracts possess antibacterial activity with remarkable antioxidant ability.
Unilateral Oculomotor Nerve Enhancement in Diabetic Oculomotor Nerve Palsy: A Retrospective Study
Authors: Zhao Juan , Li Yong , Chang Qing-Lin , Wang Jia-Wei , Sun Hou-Liang 
Published: July 2020

Abstract
Background No previous studies have explored the imaging features of oculomotor nerve palsy (ONP) in diabetic patients. In our clinical practice, isolated ONP with diabetes is partly treated with glucocorticosteroids because nerve enhancement is habitually interpreted as inflammation. Our study thus aims to summarize the imaging findings of isolated ONP with diabetes.Methods Our study included 59 patients with a clinical diagnosis of diabetic ONP. Patients were recruited from our department between January 2015 and December 2019. Orbital MRI was retrospectively analyzed for each patient, and follow-up imaging was obtained for 3 patients.Results Thickening and enhancement of the ipsilateral oculomotor nerve were detected in 38 (64.41%) patients, indicating simultaneous involvement of the cavernous segment and inferior division of the intraorbital segment. After clinically complete recovery, repeated MRI in 3 patients revealed a slightly reduced degree of enhancement of the oculomotor nerve. No differences were detected in the sex ratio (p=0.976), median onset age (p=0.563), median duration of diabetes (p=0.697), plasma glycosylated hemoglobin (HbAlc) level (p=0.278) or interval from disease onset to MRI between patients with and without enhancement of the oculomotor nerve. Conclusions Thickening and enhancement of the unilateral oculomotor nerve can be detected in patients with diabetic ONP and indicates the main involvement of the cavernous segment and the inferior division of the intraorbital segment, which may be helpful for differentiating this condition from other etiologies in isolated ONP.