bluuebunny/crossref_metadata_2025_split huggingface dataset:
Lung ultrasound in patients with rheumatoid arthritis and the definition of significant interstitial lung disease
Authors: Carlo Marco Di , Tardella Marika , Filippucci Emilio , Carotti Marina , Salaffi Fausto 
Published: May 2020

Abstract
Background. In recent years, a growing interest has grown around interstitial lung disease (ILD) in patients with rheumatoid arthritis (RA). While high resolution computed tomography (HRCT) of the chest remains the diagnostic method of choice, increasing attention has been directed towards lung ultrasound (LUS) in the diagnosis of ILD in connective tissue diseases. However, in patients with RA it is not yet clear how to interpret, in quantitative terms, the presence of B-lines, the LUS artifact indicative of ILD. The aim of this study was to determine the cut-off number of LUS B-lines that identifies a significant RA-ILD.Methods. A cross sectional study was conducted on consecutive RA patients with suspected RA-ILD. The inclusion criteria were clinical (dyspnea, velcro sounds), instrumental (suggestive anomalies on conventional radiography, DLco reduction), or in presence of at least two of the following risk factors for RA-ILD: smoking habit, male sex, advanced age, and ACPA presence.Patients underwent LUS (carried out in 14 defined intercostal spaces), chest HRCT, pulmonary function tests, and clinical evaluation. The diagnosis of RA-ILD was based on a semi-quantitative evaluation of chest HRCT using a computer-aided method (CaM). The discriminative validity of the LUS versus HRCT has been studied by using the receiver operating characteristic (ROC) curve analysis.Results. 72 consecutive RA patients (21 male, 51 female) were evaluated, with a mean age of 63.0 (SD 11.5 years). The mean estimate of pulmonary fibrosis using the CaM was 11.20% (SD 7.48) at chest HRCT, while at LUS the mean number of B-lines was 10.65 (SD 15.11). A significant RA-ILD, as measured by the CaM at HRCT, was detected in 25 patients (34.7%). The presence of 9 B-lines was found to be the optimal cut-off at ROC curve analysis. This LUS cut-off defines the presence of significant RA-ILD with a sensitivity of 70.0%, a specificity of 97.62%, and a positive likelihood ratio of 29.4.Conclusion. The present study provided data to determine the number of B-lines to identify a significant RA-ILD. LUS may represent a useful technique to select RA patients to be assessed by chest HRCT.
Expression of FAM83H and ZNF16 are associated with shorter survival of patients with gallbladder carcinoma
Authors: Ahn Sung Woo , Ahn Ae-Ri , Ha Sang Hoon , Hussein Usama Khamis , Yang Jae Do , Kim Kyoung Min , Park Ho Sung , Park See-Hyoung , Yu Hee Chul , Jang Kyu Yun 
Published: May 2020

Abstract
Background: Recently, FAM83H was reported to have roles in cancer progression in conjunction with oncogenic molecules such as MYC and b-catenin. Moreover, the data from the public database indicates a molecular relationship between FAM83H and zinc finger proteins, especially between FAM83H and ZNF16. However, studies on FAM83H and ZNF16 in gallbladder cancer have been limited. Methods: This study investigated the expression of FAM83H and ZNF16 in 105 gallbladder carcinomas. Results: In human gallbladder carcinomas, immunohistochemical expression of FAM83H was significantly associated with ZNF16 expression. In univariate analysis, nuclear and cytoplasmic expression of FAM83H or ZNF16 were significantly associated with shorter survival of gallbladder carcinoma patients. Multivariate analysis revealed the nuclear expression of FAM83H as an independent indicator of poor prognosis of overall survival (p = 0.005) and relapse-free survival (p = 0.005) of gallbladder carcinoma patients. Moreover, co-expression patterns of nuclear FAM83H and ZNF16 were also independent indicators of shorter survival of gallbladder carcinoma patients (overall survival; p < 0.001, relapse-free survival; p < 0.001). Conclusions: This study suggests FAM83H and ZNF16 are associated with the progression of gallbladder carcinoma, and the expressions of FAM83H and ZNF16 might be novel prognostic indicators of gallbladder carcinoma patients.
Using Caputo-Fabrizio Derivative For The Transmission of Mathematical Model Epidemic Corona Virus
Authors: Tahir Muhammad , Zaman Gul , Shah Syed Inayat Ali 
Published: May 2020

Abstract
In just a matter of weeks, a rapidly spreading corona virus that originated in Wuhan, China, has infected more than 20,000 people and killed at least 427 worldwide. The virus is capable of spreading from person to person, and Chinese authorities are scrambling to treat a flood of new patients successfully. The virus has spread from an initial outbreak in Wuhan, China, to 25 other countries, and has resulted in the quarantine of at least 16 Chinese cities. In this article, we considered the mathematical model [1] in which Bats-Hosts-Reservoir-People and their transmission was taken, while we introduced the population of susceptible Bats and visitors to Wuhan city or any country in same mathematical model. Now two types of population are: first in [1] introducing susceptible Bats and second visitors to Wuhan city, china or any country. We used Caputo-Fabrizio derivative with provided result that the addition of susceptible Bats and visitors are not responsible in spread of infection. The numerical result also supposed our model.
Increased Vδ1 γδT cells Predominantly Contributed to IL-17 Production in the Development of Adult Human Post-infectious Irritable Bowel Syndrome
Authors: dong liwei , sun xiaoning , ma zhichao , fu jiao , liu fujin , huang baili , liang dongchun , sun deming , lan cheng 
Published: May 2020

Abstract
Background: gdT cells play an important role in the mucosa inflammation and immunity-associated disorders. Our previous study reported that gd T cells producing IL-17 were involved in the pathogenesis of post-infectious irritable bowel syndrome (PI-IBS). However, their subset characteristic profile in this kind of disease remains unclear. Thus the current study’s aim is to investigate the functionally predominant subset and its role in PI-IBS. Methods: The total T cells were collected from the peripheral blood of patients with PI-IBS. The peripheral proportion of Vδ1 and Vδ2 subset was detected by FACS after stained with anti δ1-PE and anti δ2-APC. The local colonic proportion of this two subsets were measured under laser confocal fluorescence microscope. Vδ1 gd T cells were enriched from the total peripheral T cells by minoantibody-immuno-microbeads (MACS) method. and cultured, functionally evaluated by CCK-8 assay (proliferation), CD69/CD62L molecules expression assay (activation) and ELISA (IL-17 production) respectively.Results: 1. Vδ1 gd T cells significantly increased while Vδ2 gd T cells remained unchanged in both the peripheral blood and local colonic tissue from PI-IBS patients (P<0.05). 2. When cultured in vitro, the Vδ1 gd T cells remarkably proliferated, activated and produced IL-17 (P<0.05).Conclusions: Our results suggest that Vδ1 gd T cells was the predominant gd T cells subset in both peripheral and intestinal tissue, and was the major IL-17 producing gd T cells in PI-IBS.
Association Between Harm Reduction Strategies and Healthcare Utilization and Outcomes in Patients on Long-term Prescribed Opioid Therapy Presenting to Acute Healthcare Settings: A Systematic Review and Meta-analysis
Authors: Deschamps Jean , Gilbertson James , Straube Sebastian , Dong Kathryn , MacMaster Frank P. , Korownyk Christina , Montgomery Lori , Mahaffey Ryan , Downar James , Clarke Hance , Muscedere John , Rittenbach Katherine , Featherstone Robin , Sebastianski Meghan , Vandermeer Ben , Lynam Deborah , Magnussen Ryan , Bagshaw Sean M , Rewa Oleksa G 
Published: May 2020

Abstract
BackgroundLong-term prescription of opioids by healthcare professionals has been linked to poor individual patient outcomes and high resource utilization. Harm reduction strategies in this population in regard to acute healthcare settings may have substantial impact.MethodsWe performed a systematic review and meta-analysis of primary studies. The studies were included according to the following criteria: 1) age 18 and older; 2) long-term/chronic prescribed opioid therapy; 3) acute healthcare setting presentation from a complication of opioid therapy; 4) evaluating a harm reduction strategy; 5) comparing the effectiveness of different interventions; 6) addressing patient or healthcare related outcomes. We performed a qualitative analysis of harm reduction strategies identified. We pooled patient and system related outcome data for each harm reduction strategy.ResultsA total of 5664 studies were screened and 21 studies were included. A total of 11 broad categories of harm reduction strategies were identified. Meta-analysis was performed for the “supports for patients in pain” harm reduction strategy on two system-related outcomes using a ratio of means. The number of emergency department (ED) visits were significantly reduced for cohort studies (n=6, 0.36, 95% CI [0.20-0.62], I2 = 87%) and randomized controlled trials (RCTs) (n=3, 0.71, 95% CI [0.61-0.82], I2 = 0%). The number of opioid prescriptions at ED discharge was significantly reduced for RCTs (n=3, 0.34, 95% CI [0.14-0.82], I2 = 78%).InterpretationFor patients presenting to acute healthcare settings with complications related to long-term opioid therapy, the intervention with the most robust data is “supports for patients in pain”.Study registrationCRD42018088962
TGF-β gene polimorphisms as risk factors for asthma control among clinic patients.
Authors: Panek Michał Gabriel , Stawiski Konrad , Kuna Piotr Bolesław 
Published: May 2020

Abstract
Background:

TGF-β and its receptors play a key role in asthma pathogenesis, bronchial hyperreactivity and bronchial remodeling. Expression of isoforms 1–3 of TGFβ cytokine is influenced by tagging polymorphisms in the TGFβ1, TGFβ2 and TGFβ3 gene, and these SNPs may be associated with the risk of asthma development and severity as well as with other diseases. Polymorphic forms of TGF-β1, TGF-β2 and TGF-β3 genes regulate the degree of bronchial inflammation, deterioration of lung functional parameters in spirometry and elevated level of total IgE. All this results in intensification of disease symptoms. According to current GINA 2020 guidelines, the Asthma Control Test (ACT™) should be applied to assess asthma symptoms.
Methods:

An analysis of polymorphisms localized in TGF-β1, TGF-β2 and TGF-β3 genes was conducted on 652 DNA samples with an application of the MassARRAY® system using the mass spectrometry technique MALDI TOF MS. The degree of asthma control was evaluated with ACT™.
Results:

The occurrence of the T / C genotype in rs8109627 (p = 0.0171) in the TGF-β1 gene is significantly associated with a higher ACT result (controlled asthma) in a multivariate linear regression analysis model after using backward stepwise selection of variables. In addition, in the linear model for prediction of ACT score we showed SNP rs8109627 (p = 0.0497) in the TGF-β1 gene (improvement of the disease control - controlled asthma) and rs2796822 (p = 0.0454) in the TGF-β2 gene (deterioration of the diseases control - uncontrolled asthma) significantly modify the degree of asthma control.
Discussion:

We described clinical significance of two SNPs in two genes TGF-β1 and TGF-β2, as yet unknown. We proved that the use of both genotypes and MAC allows to create a moderately correct prognostic model which is about 70% efficient on the entire set of analyzed SNPs in TGF-β1, TGF-β2, and TGF-β3 genes.
Cost-effectiveness analysis of traditional Chinese medicine combined with antiviral drugs for hepatitis B cirrhosis based on Markov model
Authors: Wang Zhiheng , Zhang Mengpei , Zhang Haoxiang , Zuo Kaini , Pan Jie , Wang Xiaomei , Wentao Zhu 
Published: May 2020

Abstract
Background Hepatitis B cirrhosis brings a heavy burden to the health care system all over the world, and traditional Chinese medicine had a good effect in the treatment of hepatitis B cirrhosis. To correctly understand the clinical effect of Chinese medicine combined with antiviral drugs in the treatment of hepatitis B cirrhosis, the long-term therapeutic effect of traditional Chinese medicine combined with antiviral drugs in the treatment of hepatitis B cirrhosis was simulated using Markov model analysis.Methods We used four-state Markov modelling with a probabilistic cohort analysis to calculate the cost per quality-adjusted life year (QALY) gained. A one-year cycle length was applied. The best available evidence provided the model with data on the state of hepatitis B cirrhosis, risk of mortality, utility weight, and probability of transition between disease costs. A 3% discount rate was used for costs and QALYs. Cost-effectiveness acceptability curves were presented to assist decision makers.Results The model indicated that the combination of traditional Chinese medicine and antiviral therapy was cost-effective, and the result showed that there was some uncertainty. In the treatment of hepatitis B cirrhosis simulated by Markov model for 20 years, the combination of traditional Chinese medicine and anti-virus drugs increased 1.48 QALYs per patient compared with the use of antiviral drugs alone, and the total cost saved was 45,551.61 yuan. The total cost saving was mainly due to the reduction of number of hospitalizations caused by serious complications.Conclusion The combination of traditional Chinese medicine and antiviral in the treatment of hepatitis B cirrhosis could save the cost of hospitalization and improve the health output, which was a better outcome evaluated by pharmacoeconomics.
YTHDC2 Promotes the Apoptosis of Colorectal Cancer Cells Through the p38MAPK Signaling Pathway
Authors: Yu Tao , Xu YanYan , Sun Tao , Huang Di , Cao Feng , Gao Xin , Liu Gang 
Published: May 2020

Abstract
Background

The role of the m6A-binding protein YTHDC2 in the occurrence and development of colorectal cancer (CRC) is unclear. We aimed to explore the molecular mechanisms underlying this process and clarify the signaling pathway involved.
Methods

Firstly, the relationship between YTHDC2 and CRC in TCGA database was analyzed to identify relevant signaling pathways and biological processes. Then, western blot was used to analyze expression of YTHDC2 in HCT116 and Caco2 cells. After knockdown or overexpression of YTHDC2 in the above cells, RT-PCR and western blot were used to analyze p38MAPK, p-p38MAPK, and downstream apoptosis-related proteins in the MAPK signaling pathway. Flow cytometry was performed to detect changes in apoptosis.
Results

And the results were shown that the expression of YTHDC2 was significantly lower in tumor tissues than in normal tissues. Increased expression of YTHDC2 was associated with better overall survival among patients with CRC. Gene set enrichment analysis revealed that YTHDC2 regulates the MAPK signaling pathway. Flow cytometry revealed apoptosis was significantly reduced and enhanced in response to YTHDC2 knockdown and overexpression, respectively. There was no significant change in the expression of p38MAPK, while p-p38MAPK was significantly increased in response to overexpression and decreased in response to knockdown. Gene Expression Profiling Interactive Analysis showed apoptotic protein expression to be positively correlated with YTHDC2 expression, consistent with the results of RT-PCR and western blot.
Conclusion

In general, apoptosis of CRC cells is promoted by YTHDC2 via activation of the exogenous death receptor and endogenous mitochondrial apoptosis-related pathways in the p38MAPK signaling pathway.
Ratio of the Medial Hip Joint Space as a Predictor for Collapse of the Femoral Head in Non-traumatic Osteonecrosis: A Retrospective Study and Finite Element Analysis
Authors: Lin Tianye , Yang Peng , Li Hongzhu , Xu Jingli , Xiong Binglang , Chen Zhenqiu , He Wei , Wei Qiushi , Zhang Qing-Wen 
Published: May 2020

Abstract
Background: There has been no indicators that can effectively predict femoral head collapse in ONFH so far. The aim of this study is to retrospectively analyze the first-visit medial space ratio of the hip joint to evaluate its efficacy in predicting ONFH-induced collapse and impacts on the mechanical environment of necrotic femoral head.Methods: In this retrospective analysis for traditional Chinese medicine (TCM), non-traumatic osteonecrosis of femoral head (NONFH) patients from January 2010 to December 2016 were selected. The medial space ratio at their first visit and and collapse of the femoral head during the follow-up were recorded. Patients were divided into group A, B, C, D, and E according to the grading of the medial space ratio at the first visit. A total of 14 hip joint models with distinct medial space ratios were established. The maximum stress intensities of the cartilage, cortical bone and the necrotic area (N-unit area) of the femoral head in the models were quantitated using a finite element analysis.Results: One hundred twenty-eight patients (142 hips) were included in this study. The average follow-up time was 5.4±1.5 years.The Kaplan-Meier survival analysis showed that survival rates of the first-visit medial space ratios in group C and D were significantly higher (exceeding 45.76%) than that in group E (22.73%). There was no significant difference in the survival rates between group C and D (P > 0.05). The finite element analysis showed that in either the necrosis or non-necrosis group, the maximum stress in cartilage, cortical bone and the N-unit area of the femoral head increased with the medial space ratio decreasing. Conclusion: The medial space ratio shows clinical implications of predicting the collapse of NONFH except for assessing the relationship between the femoral head and the acetabulum. The stress concentrations of cartilage, cortical bone, and the necrotic area of the femoral head are enhanced with the medial space radio decreasing. Once the medial space ratio falls below 4 and continues to decline, the risk of necrotic collapse will become higher.
YAP Overexpression in Breast Cancer Cells Promotes Angiogenesis Through Activating Yap Signaling in Vascular Endothelial Cells
Authors: Yan Yu , Song Qiang , Yao Li , Zhao Liang , Cai Hui 
Published: May 2020

Abstract
Background:The YAP signaling pathway is altered and implicated as oncogenic in human mammary cancers.However, roles of YAP signaling that regulate the breast tumor angiogenesis have remained elusive. Tumor angiogenesis is coordinated by the activation of both cancer cells and vascular endothelial cells. Whether the YAP signalingpathway can regulate the intercellular interaction between cancer cells and endothelial cellsis essentially unknown.Results: We showed here that conditioned media from YAP overexpressed breast cancer cells (CM-YAP+) could promote angiogenesis, accompanied byincreased tube formation, migration, and proliferation of human umbilical vein endothelial cells (HUVECs). Down regulation of YAP in HUVECs reversed CM-YAP+ induced angiogenesis.CM-YAP+ time-dependently activated YAP inHUVECs by dephosphorylating YAP and increasing nuclear translocation.We also identified that both G13-RhoA and PI3K/Akt signaling pathway were necessary for CM-YAP+ induced activation of YAP.Besides, connective tissue growth factor (CTGF) and angiopoietin-2 (ANG-2)actedas down-stream of YAP in HUVECs to promote angiogenesis.In addition, subcutaneous tumors nude mice model demonstrated that tumors overexpressed YAP revealed moreneovascularization in vivo.Conclusions: YAP-YAP interaction between breastcancer cells and endothelial cellscould promote tumor angiogenesis, supporting that YAP is a potential marker and target fordeveloping novel therapeutic strategies against breast cancer.
Twin-Chain Polymer Networks Loaded With Nanostructured Fluids for the Selective Removal of A Non-Original Varnish From A 20th Century Oil Painting At The Peggy Guggenheim Collection, Venice
Authors: Buemi Luciano Pensabene , Petruzzellis Maria Laura , Chelazzi David , Baglioni Michele , Mastrangelo Rosangela , Giorgi Rodorico , Baglioni Piero 
Published: May 2020

Abstract
This paper reports on the evaluation of a polyvinyl alcohol (PVA) “twin-chain” polymer network (TC-PN) combined with an oil-in-water nanostructured fluid (NSF) for the removal of a polyvinyl acetate (PVAc) varnish. Small Angle X-ray Scattering, Confocal Laser Scanning Microscopy, and Fluorescence Correlation Spectroscopy showed that the structure of the gel and the NSF are only minimally altered by loading the fluid into the gel. The NSF is partially free to diffuse through the network, but also interacts with the gel walls. During the cleaning, the dynamics of the fluid at the gel-substrate interface are controlled by the osmotic balance taking place among the interconnected pores. These features grant effective and controlled cleaning performances. The case study identified for this research is Pablo Picasso’s The Studio (L’Atelier, 1928), one of the masterpieces in the Peggy Guggenheim Collection, Venice (PGC). In 1969 the oil painting, originally unprotected, was wax-lined and then varnished using a PVAc varnish. Over the years, the white shades of the painting have been compromised by the yellowing of the varnish and soiling of deposits. On painting mock-ups, the NSF-loaded hydrogels allowed the swelling and softening of PVAc varnish and wax layers, which were then removed with gentle mechanical action. Effective varnish and wax removal at the micron scale, and the absence of residues from the cleaning system (gel and NSF), were confirmed by Fourier Transform Infrared Spectroscopy (FTIR) 2D imaging. The effective and safe removal of the aged PVAc varnish and wax layer from the surface of the painting was then carried out using the same cleaning protocol successfully tested on the mock-ups, setting the NSF-loaded PVA TC-PNs as robust and reliable tools for the cleaning of sensitive works of art.
Time Series Modeling of Air Pollution and Its Association with Season and Climate Variables in Istanbul, Turkey
Authors: al-najjar hazem , Al-Rousan Nadia , Elhaty Ismail A. 
Published: May 2020

Abstract
Air pollution depends on seasons, wind speed, temperature, wind direction and air pressure. The effect of different seasons on air pollution is not fully addressed in the reported works. The current study investigated the impact of season on air pollutants including SO2, PM10, NO, NOX, and O3 using NARX method. In the applied methodology, a feature selection was used with each pollutant to find the most important season(s). Afterward, six models are designed based on the feature selection to show the impact of seasons in finding the concentration of pollutants. A case study is conducted on Esenyurt which is one of the most populated and industrialized places in Istanbul to validate the proposed framework. The performance of using all of the designed models with different pollutants showed that using season effect led to improving the performance of predictor and generating high R2 and low error functions.