bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparison of benchtop and handheld near‐infrared spectroscopy devices to determine forage nutritive value
Authors: Acosta J. J. , Castillo M. S. , Hodge G. R. 
Published: July 2020

AbstractThe quality of predicted plant‐, soil‐, and animal‐response values from near‐infrared (NIR) reflectance spectra depends on the ability to generate appropriate NIR models. The first step in the development of NIR models is collection of spectral data. Limited work, however, has been reported that compares NIR models for prediction of forage nutritive value when the spectra are obtained from devices with different spectral ranges and resolutions. The objectives of this study were to (a) develop and evaluate NIR spectroscopy models using a benchtop‐type (FOSS) and two handheld NIR devices (microPHAZIR and DLP NIRscan Nano EVM) to predict crude protein (CP), acid detergent fiber (ADF), amylase and sodium sulfite‐treated neutral detergent fiber (aNDF), and in vitro true dry matter digestibility (IVTD) of dried ground forage grass samples and (b) compare predictions among the three NIR devices. Switchgrass (Panicum virgatum L.) and bermudagrass [Cynodon dactylon (L.) Pers] hay samples were scanned with the NIR devices and analyzed with wet chemistry for development of NIR prediction models. Among devices, the r2 of validation values for aNDF models ranged from .81 to .87; all other r2 values were >.86 and as high as .98 with standard error of prediction (SEP; g kg−1) ranging from 8.1 to 11.5 for CP, 19.1 to 23.8 for aNDF, 14.2 to 20.0 for ADF, and 26.8 to 49.9 for IVTD. The FOSS benchtop NIR prediction models consistently had the highest r2 and lowest SEP values; however, the predictive power for both handheld devices was similar to the benchtop‐type device.
Clippings return decreases mineral nitrogen requirements for bermudagrass (Cynodon spp.) lawns in Mediterranean Europe
Authors: Schiavon Marco , Pornaro Cristina , Macolino Stefano 
Published: July 2020

AbstractThe use of bermudagrass [Cynodon dactylon (L.) Pers.] and hybrid bermudagrass (C. dactylon x C. transvaalensis Burtt Davy) in lawns is rapidly increasing in Mediterranean Europe; however, the identification of optimal N fertilization practices is needed to shorten the long dormancy periods some cultivars undergo in these environments. A 2‐yr study was conducted at the agricultural experimental farm of Padova University from May 2016 until June 2018 to compare the effects of three N fertilization rates (160 kg N ha−1 without clippings returned, 80 kg N ha−1 with clippings returned, and 80 kg N ha−1 without clippings returned applied with a controlled‐release fertilizer) on two bermudagrass cultivars (‘La Paloma’, ‘Yukon’) and two hybrid bermudagrass cultivars (‘Patriot’, ‘Tifway’) by measuring summer and fall quality, spring green‐up, and root morphology. Bermudagrasses fertilized at 160 kg N ha−1 without clippings returned slightly increased turfgrass quality in the summer, but higher turf quality was recorded in plots fertilized at 80 kg N ha−1 with clippings returned after weekly mowing events in the fall. The same N rates had a positive effect on spring green‐up for ‘La Paloma’ and ‘Tifway’; however, no benefits of increased N rates were detected on root morphology. Results suggest that returning clippings can be a powerful tool for reducing mineral N applications and increase growing season length in northern Italy.
Deep Eutectic Solvent Extraction of High‐Purity Lignin from a Corn Stover Hydrolysate
Authors: Cronin Dylan J. , Chen Xiaowen , Moghaddam Lalehvash , Zhang Xiao 
Published: July 2020

AbstractA lactic acid/chlorine chloride‐based deep eutectic solvent (DES) was used for the extraction of high‐purity lignin (up to 94.7 %) in high yield (up to 75 %) from the hydrolysis/fermentation residue corn stover hydrolysate (CSH), which was generated from a pilot‐plant‐scale biorefinery. A range of extraction conditions were investigated, which involved varying reaction temperature, time, and DES composition. The relationship between lignin yield, purity, and structural characteristics with DES treatment conditions was determined. The extraction of high‐purity lignin from hydrolysis/fermentation residues presents a promising approach for enhancing the economic feasibility of a lignocellulose biorefinery. It was also determined that DES extraction can produce lignin with a controlled range of molecular weight and functional group content.
Advances in Organic Anode Materials for Na‐/K‐Ion Rechargeable Batteries
Authors: Desai Aamod V. , Morris Russell E. , Armstrong A. Robert 
Published: July 2020

AbstractElectrochemical energy storage (EES) devices are gaining ever greater prominence in the quest for global energy security. With increasing applications and widening scope, rechargeable battery technology is gradually finding avenues for more abundant and sustainable systems such as Na‐ion (NIB) and K‐ion batteries (KIB). Development of suitable electrode materials lies at the core of this transition. Organic redox‐active molecules are attractive candidates as negative electrode materials owing to their low redox potentials and the fact that they can be obtained from biomass. Also, the rich structural diversity allows integration into several solid‐state polymeric materials. Research in this domain is increasingly focused on deploying molecular engineering to address specific electrochemical limitations that hamper competition with rival materials. This Minireview aims to summarize the advances in both the electrochemical properties and the materials development of organic anode materials.
Good taste or gut feeling? A new method in rats shows oro‐sensory stimulation and gastric distention generate distinct and overlapping brain activation patterns
Authors: Roelofs Theresia J. M. , Luijendijk Mieneke C. M. , van der Toorn Annette , Camps Guido , Smeets Paul A. M. , Dijkhuizen Rick M. , Adan Roger A. H. 
Published: July 2020

AbstractSatiation is influenced by a variety of signals including gastric distention and oro‐sensory stimulation. Here we developed a high‐field (9.4 T) functional magnetic resonance imaging (fMRI) protocol to test how oro‐sensory stimulation and gastric distention, as induced with a block‐design paradigm, affect brain activation under different states of energy balance in rats. Repeated tasting of sucrose induced positive and negative fMRI responses in the ventral tegmental area and septum, respectively, and gradual neural activation in the anterior insula and the brain stem nucleus of the solitary tract (NTS), as revealed using a two‐level generalized linear model‐based analysis. These unique findings align with comparable human experiments, and are now for the first time identified in rats, thereby allowing for comparison between species. Gastric distention induced more extensive brain activation, involving the insular cortex and NTS. Our findings are largely in line with human studies that have shown that the NTS is involved in processing both visceral information and taste, and anterior insula in processing sweet taste oro‐sensory signals. Gastric distention and sucrose tasting induced responses in mesolimbic areas, to our knowledge not previously detected in humans, which may reflect the rewarding effects of a full stomach and sweet taste, thereby giving more insight into the processing of sensory signals leading to satiation. The similarities of these data to human neuroimaging data demonstrate the translational value of the approach and offer a new avenue to deepen our understanding of the process of satiation in healthy people and those with eating disorders.
BRCC36 functions noncatalytically to promote antiviral response by maintaining STAT1 protein stability
Authors: Cheng Qiao , Feng Qian , Xu Yang , Zuo Yibo , Liu Jin , Yuan Yukang , Miao Ying , Liu Yin , Lei Lei , Guo Tingting , Zhang Liting , Wu Depei , Zheng Hui 
Published: July 2020

AbstractViral infection is a serious threat to both normal population and clinical patients. STAT1 plays central roles in host defense against viral infection. How STAT1 protein maintains stable in different conditions remains largely unknown. Here, we identified BRCC36 as a potent regulator of STAT1 protein stability. Mechanistically, BRCC36 maintains STAT1 levels by utilizing USP13 to form a balanced complex for antagonizing Smurf1‐mediated degradation. Importantly, cellular BRCC36 deficiency results in rapid downregulation of STAT1 during viral infection, whereas a supplement of BRCC36 maintains STAT1 protein levels and host antiviral immunity in vivo. Moreover, we revealed that BRCC36 expression was downregulated in allogeneic HSC transplantation (allo‐HSCT) mice that showed increased susceptibility to viral infection. Supplementing BRCC36 enhanced antiviral response of allo‐HSCT mice by maintaining STAT1 stability. This study uncovers a critical role of BRCC36 in STAT1 protein stability and could provide potential strategies for enhancing clinical antiviral therapy.
Intestinal microbiota disruption limits the isoniazid mediated clearance of Mycobacterium tuberculosis in mice
Authors: Negi Shikha , Pahari Susanta , Bashir Hilal , Agrewala Javed N. 
Published: July 2020

AbstractTuberculosis (TB) continues to remain a global threat due to the emergence of drug‐resistant Mycobacterium tuberculosis (Mtb) strains and toxicity associated with TB drugs. Intestinal microbiota has been reported to affect the host response to immunotherapy and drugs. However, how it affects the potency of first‐line TB drug isoniazid (INH) is largely unknown. Here, we examined the impact of gut microbial dysbiosis on INH efficiency to kill Mtb. In this study, we employed in vivo mouse model, pretreated with broad‐spectrum antibiotics (Abx) cocktail to disrupt their intestinal microbial population prior to Mtb infection and subsequent INH therapy. We demonstrated that microbiota disruption results in the impairment of INH‐mediated Mtb clearance, and aggravated TB‐associated tissue pathology. Further, it suppressed the innate immunity and reduced CD4 T‐cell response against Mtb. Interestingly, a distinct shift of gut microbial profile was noted with abundance of Enterococcus and reduction of Lactobacillus and Bifidobacterium population. Our results show that the intestinal microbiota is crucial determinant in efficacy of INH to kill Mtb and impacts the host immune response against infection. This work provides an intriguing insight into the potential links between host gut microbiota and potency of INH.
Dual Responsive Disposable Electrode for the Enumeration of Escherichia coli in Whole Blood
Authors: Panhwar Sallahuddin , Ilhan Hasan , Hassan Syeda Sara , Zengin Adem , Boyacı Ismail Hakkı , Tamer Ugur 
Published: July 2020

AbstractIn this report, we have designed a dual responsive disposable electrode for the enumeration of Escherichia coli K12 (E. coli K12). The immunomagnetic separation strategy provided easily bacterial detection in the whole blood. Metal‐organic frameworks magnetic (MOFs) modified, and citrate capped gold nanoparticles were used as capture probe and spectro‐electrochemical labels, respectively. The cyclic voltammetry (CV) was employed to quantify E. coli with a ranging from 101 to 107 cfu/mL with a LOD of 1 cfu/mL. The Surface enhanced Raman spectroscopy (SERS) measurements were also performed with using disposable electrode.
Debating responsibility‐sharing: An analysis of the European Parliament's debates on the Common European Asylum System
Authors: Kaufmann David 
Published: July 2020

AbstractThe Common European Asylum System (CEAS) has failed to ensure effective protection in times of high numbers of asylum seekers. CEAS reforms have not successfully introduced responsibility‐sharing mechanisms that can balance the effects of the Dublin Regulation's allocation rules. This paper analyzed the absence of substantial CEAS reforms by examining the discursive strategies used by Members of the European Parliament (MEPs) in asylum reform debates during the European Parliament's (EP) seventh legislative term (2009–2014). The mixed‐methods analysis revealed that: (a) MEPs across the political spectrum argued in favor of responsibility‐sharing, (b) MEPs from south‐eastern border European Union Member States were prone to use solidarity discursive strategies to speak in favor of responsibility‐sharing, (c) and that MEPs aligned their discursive strategies to the actual responsibility‐sharing mechanisms under discussion. These findings point to substantive and problem‐oriented debates in the EP. Yet, these debates did not translate into essential CEAS reforms.
Diagnosis of digestive system tumours
Authors: Washington Mary Kay , Goldberg Richard M. , Chang George J. , Limburg Paul , Lam Alfred K. , Salto‐Tellez Manuel , Arends Mark J. , Nagtegaal Iris D. , Klimstra David S. , Rugge Massimo , Schirmacher Peter , Lazar Alexander J. , Odze Robert D. , Carneiro Fatima , Fukayama Masashi , Cree Ian A. ,   WHO Classification of Tumours Editorial Board
Published: July 2020

AbstractThe WHO Classification of Tumours provides the international standards for the classification and diagnosis of tumours. It enables direct comparisons to be made between different countries. In the new fifth edition, the series has gone digital with the launch of a website as well as a series of books, known widely as the WHO Blue Books. The first volume to be produced is on the classification of Digestive System tumours, replacing the successful 2010 version. It has been rewritten and updated accordingly. This article summarises the major diagnostic innovations that have occurred over the last decade and that have now been incorporated in the classification. As an example, it incorporates the recently proposed classification of neuroendocrine tumours, based on the recognition that neuroendocrine tumours and carcinomas differ substantially in the genetic abnormalities that drive their growth, findings relevant to treatment selection and outcome prediction. Several themes have emerged during the production process. One is the importance of the progression from hyperplasia to dysplasia to carcinoma in the evolution of the malignant process. Advances in imaging techniques and endoscopy have resulted in enhanced access to precancerous lesions in the gastrointestinal and biliary tract, necessitating both changes in classification schema and clinical practice. Diagnosis of tumours is no longer the sole purview of pathologists, and some patients now receive treatment before tissue is obtained, based on clinical, radiological and liquid biopsy results. This makes the classification relevant to many disciplines involved in the care of patients with tumours of the digestive system.
Category of evidence and consensus underlying National Comprehensive Cancer Network guidelines: Is there evidence of progress?
Authors: Desai Aakash P. , Go Ronald S. , Poonacha Thejaswi K. 
Published: July 2020

AbstractNational Comprehensive Cancer Network (NCCN) guidelines are the most comprehensive and widely used standard for clinical care, financial reimbursements and quality improvement initiatives in oncology. We studied the distribution of categories of evidence and consensus (EC) in the guidelines for the common cancers in the United States. We evaluated the EC categories in staging, therapy and surveillance recommendations in 2019 guidelines and compared them with the same in 2010. The latest 2019 version of NCCN guidelines were obtained. The definitions for various categories of EC used were, Category 1 (high level evidence, uniform consensus), Category 2A (lower level of evidence [LOE], uniform consensus), Category 2B (lower LOE, no uniform consensus but with no major disagreement) and Category 3 (any LOE, major disagreement). We compared our results with previously published results from 2010 guidelines. Total number of recommendations increased by 77% from 1023 (2010) to 1818 (2019). Of the 1818 recommendations, Category 1, 2A, 2B and 3 EC were 7%, 87%, 6% and 0%, respectively, while in 2010 they were 6%, 83%, 10% and 1%. Breast (30%), lung (10%) and kidney (10%) cancer had the highest proportions of Category 1 therapeutic recommendations in their respective guidelines. No Category 1 recommendations were found in screening or surveillance guidelines or in pancreatic and uterine cancer guidelines. Recommendations in 2019 NCCN guidelines are largely Category 2A (lower levels of evidence, uniform expert opinion), unchanged from the previous study in 2010.
Serological chemiluminescence immunoassay for the diagnosis of SARS‐CoV‐2 infection
Authors: Lijia Shao , Lihong Shen , Huabin Wang , Xiaoping Xu , Xiaodong Lu , Yixuan Zhu , Pin He , Yina Xu , Xiaoyun Shan , Junqi Wu 
Published: July 2020

AbstractObjectiveDynamic monitoring of the concentration variation of IgM and IgG in patients with SARS‐CoV‐2 infections and exploring their diagnostic value for coronavirus disease‐19 (COVID‐19).MethodsA total of 15 patients with SARS‐CoV‐2 infection were enrolled as the COVID‐19 group, and 50 patients were enrolled as the control group. The concentrations of SARS‐CoV‐2‐specific antibodies (IgM and IgG) were detected by a chemiluminescence immunoassay (CLIA).ResultsAccording to the cutoff value recommended by the manufacturer (cutoff = 10 AU/mL), the sensitivity, specificity, Youden index (YI), positive predictive value (PPV), and negative predictive value (NPV) of IgM were 60%, 100%, 60%, 100%, and 89.29%, respectively; and 86.67%, 100%, 86.67%, 100%, and 96.15%, respectively, for IgG. We reassessed the cutoff value of IgM. When the cutoff value for SARS‐CoV‐2 IgM was 1.83 AU/mL, the sensitivity, specificity, YI, PPV, and NPV were 93.33%, 98%, 91.33%, 93.33%, and 98%, respectively. During dynamic monitoring of the concentrations of IgM and IgG in COVID‐19 patients, we found the shortest times before a patient became IgM and IgG seropositive after symptom onset were 1.5 and 2 days, respectively. The longest times were 7 and 8 days, respectively. The positive rates of SARS‐CoV‐2 IgM and IgG both reached 100% in 8‐14 days after symptom onset.ConclusionThe IgM cutoff value of 1.83 AU/mL for the diagnosis of COVID‐19 was much better than the cutoff suggested by the manufacturer. SARS‐CoV‐2 infection can be ruled out if antibodies against SARS‐CoV‐2 are still undetectable 14 days after symptom onset.
Association between detectable SARS‐COV‐2 RNA in anal swabs and disease severity in patients with coronavirus disease 2019
Authors: Lin Weiyin , Xie Zhiwei , Li Yueping , Li Liya , Wen Chunyan , Cao Yi , Chen Xiaoting , Ou Xu , Hu Fengyu , Li Feng , Tang Xiaoping , Cai Weiping , Li Linghua 
Published: July 2020

AbstractBackgroundSevere acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) RNA was found in the intestines and feces, but its clinical significance is not completely clear. We aim to characterize the longitudinal test results of SARS‐CoV‐2 RNA in anal swabs and to explore the association with disease severity.MethodsWe included laboratory‐confirmed coronavirus disease 2019 (COVID‐19) patients, who were hospitalized in Guangzhou Eighth People's Hospital and excluded those who had not received anal swabs for SARS‐COV‐2 RNA testing. Epidemiological, clinical, and laboratory data were obtained. Throat swabs and anal swabs were collected periodically for SARS‐COV‐2 RNA detection.ResultsTwo hundred and seventeen eligible patients (median aged 50 years, 50.2% were females) were analyzed. 21.2% (46/217) of the patients were detected with SARS‐CoV‐2 RNA in anal swabs. The duration of viral RNA was longer, but the viral load was lower in anal swabs than throat swabs in the early stage of the disease. During a median follow‐up of 20 days, 30 (13.8%) patients were admitted to the intensive care unit (ICU) for high‐flow nasal cannula or higher‐level oxygen support measures to correct hypoxemia. Detectable viral RNA in anal swabs (adjusted hazard ratio [aHR], 2.50; 95% confidence interval [CI], 1.20‐5.24), increased C‐reactive protein (aHR, 3.14; 95% CI, 1.35‐7.32) and lymphocytopenia (aHR, 3.12; 95% CI, 1.46‐6.67) were independently associated with ICU admission. The cumulative incidence of ICU admission was higher among patients with detectable viral RNA in anal swabs (26.3% vs 10.7%, P = .006).ConclusionDetectable SARS‐CoV‐2 RNA in the digestive tract was a potential warning indicator of severe disease.
Tocilizumab for the treatment of adult patients with severe COVID‐19 pneumonia: A single‐center cohort study
Authors: Fernández‐Ruiz Mario , López‐Medrano Francisco , Pérez‐Jacoiste Asín María Asunción , Maestro de la Calle Guillermo , Bueno Héctor , Caro‐Teller José Manuel , Catalán Mercedes , de la Calle Cristina , García‐García Rocío , Gómez Carlos , Laguna‐Goya Rocío , Lizasoáin Manuel , Martínez‐López Joaquín , Origüen Julia , Pablos José Luis , Ripoll Mar , San Juan Rafael , Trujillo Hernando , Lumbreras Carlos , Aguado José María 
Published: July 2020

AbstractCoronavirus disease 2019 (COVID‐19) can lead to a massive cytokine release. The use of the anti‐interleukin‐6 receptor monoclonal antibody tocilizumab (TCZ) has been proposed in this hyperinflammatory phase, although supporting evidence is limited. We retrospectively analyzed 88 consecutive patients with COVID‐19 pneumonia that received at least one dose of intravenous TCZ in our institution between 16 and 27 March 2020. Clinical status from day 0 (first TCZ dose) through day 14 was assessed by a 6‐point ordinal scale. The primary outcome was clinical improvement (hospital discharge and/or a decrease of ≥2 points on the 6‐point scale) by day 7. Secondary outcomes included clinical improvement by day 14 and dynamics of vital signs and laboratory values. Rates of clinical improvement by days 7 and 14 were 44.3% (39/88) and 73.9% (65/88). Previous or concomitant receipt of subcutaneous interferon‐β (adjusted odds ratio [aOR]: 0.23; 95% confidence interval [CI]: 0.06‐0.94; P = .041) and serum lactate dehydrogenase more than 450 U/L at day 0 (aOR: 0.25; 95% CI: 0.06‐0.99; P = .048) were negatively associated with clinical improvement by day 7. All‐cause mortality was 6.8% (6/88). Body temperature and respiratory and cardiac rates significantly decreased by day 1 compared to day 0. Lymphocyte count and pulse oximetry oxygen saturation/FiO2 ratio increased by days 3 and 5, whereas C‐reactive protein levels dropped by day 2. There were no TCZ‐attributable adverse events. In this observational single‐center study, TCZ appeared to be useful and safe as immunomodulatory therapy for severe COVID‐19 pneumonia.
Regional differences in the effects of the ablation index and interlesion distance on acute electrical reconnections after pulmonary vein isolation
Authors: Yazaki Kyoichiro , Ejima Koichiro , Higuchi Satoshi , Yagishita Daigo , Shoda Morio , Hagiwara Nobuhisa 
Published: July 2020

AbstractBackgroundIn pulmonary vein isolation, the regional differences in the ablation index (AI) and interlesion distance (ILD) remain unclear. This study aimed to evaluate the association between the AI, ILD, and other relevant indices with pulmonary vein reconnections (PVRs) during the surgical intervention with a focus on the heterogeneous regional variability through a retrospective analysis.MethodsWe divided the wide area circumferential ablation (WACA) region into 12 segments in 32 consecutive patients, which resulted in a 384 segment analysis to evaluate the association of the minimum AI (AI min) and maximum ILD (ILD max) with acute PVRs, which were defined as spontaneous PVRs or dormant conduction after adenosine triphosphate administration.ResultsAcute PVRs were observed in 48 (13%) segments and 40 (63%) WACA regions. The AI min was significantly lower and ILD max greater in segments with PVRs than in those without (372 vs 403 au and 6.5 vs 5.7 mm, respectively). PVRs were more frequent in the left posterior segments, adjacent to the esophagus, than in other segments (23% vs 10%, respectively). Notably, ILD max was significantly greater in the left posterior segments with acute PVRs with AI min < 297 (median; 6.5 vs 5.1 mm); a similar finding was not observed when with AI min ≥ 297.ConclusionSmaller ILD may prevent acute PVRs when the AI min is low in the left posterior segments.