bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparison of consumed portion sizes and serving sizes in UK energy dense snack foods
Authors: Rippin Holly , Hutchinson Jayne , Jewell Jo , Breda Joao , Cade Janet 
Published: June 2020

AbstractObesity is a major health problem facing the European population; over two thirds (67%) of UK adult men and 58% women are overweight or obese (1). The positive association between on-pack serving-size and food intake is known as the ‘portion size effect’. However, although direct links with obesity remain unproven, evidence suggests that limiting consumed portion size contributes to reduced energy intake and therefore reduced weight gain (2). UK portion size guidance is outdated and evidence suggests that on-pack serving-sizes have increased in some energy-dense foods (3). This study explores consumed portion sizes and on-pack serving-sizes in popular energy, fat and sugar-dense foods.The UK National Diet & Nutrition Survey 2008–2014 (n = 2377) dataset was used to identify commonly consumed energy, fat and sugar-dense foods. Data was analysed for adults aged 19–64y (excluding under-reporters) for consumed portion sizes, and a commercial product database of major UK retailer and manufacturer data provided serving-sizes. Commonly consumed energy, fat and sugar-dense food groups were split into 45 product-based subgroups. Means of consumed portion size and on-pack serving-size were calculated and compared and nutrition per 100 g and per serve was explored.Just 57% products had serving-size details, whereas 97% had pack-size information; Chocolate had the least products with serving-size information (35%). Lack of on-pack serving-size guidance is therefore a widespread issue, particularly in some energy-dense snack foods. Serving-size ranges were wide and varied across food groups. Consumed portion sizes were significantly higher than on-pack serving-size in all main food groups and most subgroups. The greatest difference between consumed portion size and on-pack serving-size was Crisps (44%), and within this, ‘popcorn’ (151%). There is a real need for policies aimed at setting product pack and serving-sizes that help individuals consume smaller portions. However, further consideration is needed on how consumers understand on-pack serving-size messaging, front-of-pack labelling and pack size.Serving-size was unavailable for many products. However, where available, consumed portion sizes were higher than on-pack serving-size in all main food groups and most subgroups. These results could inform updated portion size guidance of energy-dense foods. Further work is needed to clarify whether smaller serving and pack sizes would lead to lower total consumption and energy/nutrient intake.
Consumers’ distrust about E-numbers: a qualitative study among food experts
Authors: Van Gunst Annelies , Roodenburg Annet JC 
Published: June 2020

IntroductionConsumers demand healthy food products. While E-numbers are allowed in these products, many consumers have a negative perception. Food companies act on this and communicate on their package about removing E-numbers from their products or using less ‘chemical-sounding’ terms (clean label). Consequently, the question among food experts is how to deal with this perception and the real effects on health.The objective was to study the opinion of food experts about the causes and ways to reduce consumers’ distrust about E-numbers.MethodsThirteen food experts from universities, research institutes, Ministry of Health, Welfare and Sport, food industry organizations, media, The Netherlands Nutrition Centre, Dutch Consumers’ Association and two other non-governmental organizations (NGOs) were interviewed with a semi-structured topic list, based on a model of risk perception. Interviews were transcribed, coded by an open coding approach and analyzed.Results & discussionResults indicated that, according to food experts, this distrust of consumers arose from negative communication in the past and nowadays by the (social) media and in books. In this communication, the information source and the reliability of E-number information are important for consumers, according to food experts. Food experts suggested to reduce the distrust by avoiding negative claims on the label such as no/ reduced E-numbers, and to make collective appointments with all parties about a honest and transparent communication to consumers. Food companies need to explain clear and honest why they use E-numbers in food. The Netherlands Nutrition Centre and the Ministry of Health, Welfare and Sport were often mentioned as parties to undertake action, consumers had no confidence in the food industry, according to the experts.
Dietary intake of vitamin D in adults with overweight and obesity
Authors: Nikolova Maria , Penkov Alexander 
Published: June 2020

AbstractIntroductionThe aim of the present study is to study in detail the dietary intake of vitamin D in subjects with different BMI in relation to the status of serum vitamin D.Material and MethodsThey have been studied 264 individuals (41.3% were men and 58.9% were women), aged 19 to 60 years. Body weight and height, waist circumference, systolic and diastolic blood pressure were recorded. Body composition was assessed by bioelectrical impedance. Serum 25(OH)D Total, insulin,high-sensitivity C-reactive protein, glucose level and lipids were measured. Nutrition evaluation was performed through a 24-hour recall and FFQ in January-April 2014 and 2015.Results27.2 % of the participants had normal weight, 24.6 % - overweight, 29.2 % - class I obesity, and 18.9 % – class II or III. 33.3 % had vitamin D deficiency, 40.2 % - insufficiency. It was found that the average daily intake of vitamin D for the whole group of subjects was 6.6 mcg/day. (for the women - 5.6 μg/d and for the men - 10.4 μg/d). In BMI-defined groups it was found that daily average daily intakes of vitamin D in subjects with normal BMI were 7.6 mcg / day, in those with overweight was 6.6 mcg / d, and in those with obesity was 6.0 mcg / day. 80.6% of the subjects were found to have daily average daily intakes of vitamin D under the EAR, which determines a high relative share of individuals with a potential risk of vitamin D deficiency. We found significant, mild to moderate correlations between daily average dietary intake of vitamin D and weight, % body fat (% BF); fat mass (FM), visceral fat (in women), muscle and fat-free mass (FFM), and height-adjusted indices : fat mass index (FMI) and fat-free mass index (FFMI), total body water (TBW in kg and %) and with some of metabolic variables (basal insulin and with the chronic inflammation marker - hs-CRP.DiscussionDietary intake of vitamin D is insufficient and correlates with serum vitamin D levels, FM(kg), %FM, visceral fat, FFM, muscle mass and some of metabolic variables. Male sex, higher education, younger than 30 years and normal BMI (up to 25.0 kg/m2) are the factors determining the higher dietary intake of vitamin D. This requires targeted supplementation and additional intake of vitamin D fortified foods to individuals at risk.
Intakes and sources of menaquinones (vitamin K2) in the Irish population aged 1–90 years
Authors: Kingston Ciara , Kehoe Laura , Walton Janette , McNulty Breige , Nugent Anne , Cashman Kevin , Flynn Albert 
Published: June 2020

AbstractRecent evidence suggests that menaquinones (Vitamin K2) may be important for both bone health and cardiovascular health. With the exception of menaquinone-4 which is formed from the tissue specific conversion of phylloquinone or menadione, menaquinones are synthesized by bacteria. They are typically found in foods of animal origin such as meat, dairy and fermented foods and may account for up to 25% of total vitamin K intake. There are few data available on menaquinone intakes in population groups due to lack of available composition data. The Irish food composition database however has recently been updated to include data on menaquinones. Therefore, the aim of this study was to use these data to estimate the intakes and key dietary sources of menaquinones (menaquinone-4 & menaquinone-5–10) in the Irish population.Analyses included data from four nationally representative surveys of the Irish population; The National Pre-School Nutrition Survey (1–4y; 2010–2011), the National Children's Food Survey (5–12y; 2003–2004), the National Teens’ Food Survey (13–17y; 2005–2006) and the National Adult Nutrition Survey (18–90y; 2008–2010) (www.iuna.net). For all of the surveys, food and beverage intake data (including supplements) were collected using food records and quantified using food scales, photographic atlases, household measures and standard portion sizes. Mean daily intakes of menaquinone-4 and menaquinone-5–10 were estimated using UK food composition tables and analytical values from published papers. The percent contribution of food groups to menaquinone intake was calculated by the mean proportion method (which provides information about the sources that are contributing to the nutrient intake ‘per person’). Statistical analyses were carried out using SPSS© V22.Mean intakes of menaquinone-4 ranged from 8–12μg/d and 10–14μg/d in children aged 1–17 years and adults aged 18–90 years, respectively. Mean combined intakes of menaquinone-5–10 ranged from 32–43μg/d and 32–50μg/d in children aged 1–17 years and adults aged 18–90 years, respectively. ‘Meat & meat products’ were the top contributor of menaquinone-4 (50–66%) intakes across all population groups followed by ‘milks’ (9–25%). ‘Meat & meat products’ (44–61%) and ‘cheeses’ (21–37%) were important contributors to menaquinone-5–10 intakes across all population groups examined.This study is the first of its kind to report the intakes and sources of menaquinones in the Irish population. ‘Meat & meat products’, ‘cheeses’ and ‘milks’ are important contributors to intakes of menaquinones in the Irish diet. Further research is required to fully understand the role of the menaquinones in human health.
Cow's milk exclusion in young children: Parents motives, sources of information and perception of their child's eating behaviours
Authors: Steadman Francesca , Houston-Price Carmel , Clegg Miriam 
Published: June 2020

AbstractResearch has assessed the diets of young children that follow a diet that excludes cow's milk. However, there is insufficient information about why parents decide to exclude cow's milk, where they obtain nutritional information, and their child's eating behaviours. This is important as the prevalence of cow's milk allergy is increasingly common in children, alongside a growing preference for plant-based diets. The purpose of this research was firstly to explore why parents exclude cow's milk from their child's diets and if this is due to recommendations from a health care professional. Secondly, to understand where parents source information to enable them to provide a healthy diet for their child. Finally, to explore parent's opinions of their child's eating behaviours.Thirty parents of children aged 1–3 years who exclude cow's milk and 20 parents of children who did not follow a restricted diet completed an online questionnaire. The questionnaire comprised of three blocks of questions. The first asked about the parents, the second pertained to the child. If they excluded cow's milk they were asked why they made this decision and who advised them. Parents were also asked where they source nutrition information for their child. The final block of questions consisted of the Child Eating Behaviour Questionnaire.Parents reported excluding cow's milk due to cow's milk allergy (60%), lactose or milk intolerance (10%), suspected allergy or intolerance (27%) or personal choice (3%). When asked “Who advised you to remove cow's milk from your child's diet?” the majority responded that it was their own decision (35%), followed by GP advice (24%). The most common sources of nutrition information for parents of children that exclude cow's milk came from parenting webpages (21%), and social media (19%), whilst those on an unrestricted diet obtained information from health visitors (24%) and parenting webpages (19%). There were no differences in eating behaviours between children that excluded cow's milk and children that did not except for satiety responsiveness (p = 0.014), which was greater in the cow's milk exclusion group.This preliminary data indicates that many parents exclude cow's milk from their child's diets based on their own advice. Where parents of children on an unrestricted diet sourced nutrition information from their health visitor, few parents of children who exclude cow's milk used this source of support (7%).
The EnergyKids project: pilot study on the energy balance of primary school children during school days and summer camp days
Authors: Rosi Alice , Franchini Cinzia , Scazzina Francesca 
Published: June 2020

AbstractSpecific nutritional requirements should be cover during childhood to ensure proper growth. The daily energy need is defined as the number of calories necessary to satisfy the total energy expenditure in a healthy and active organism. Since children are more inclined to be physically active during summer, their energy expenditure may vary during school days respect to summer days and a proper energy intake should be planned during both periods.The primary aim of this study was to evaluate the energy balance during school days and summer camp days in primary school children living in the city of Parma (Italy), attending the Giocampus programme.Participants were asked to complete a 3-day weighed food diary and to wear an activity tracker for the same 3 consecutive days twice: within a school week and a summer camp week. Height and body weight were measured at the beginning of each assessment week and BMI was calculated to define the weight status through the IOTF gender- and age-related cut-offs for children BMI. The mean energy balance was estimated as the difference between the mean total energy expenditure (calculated multiplying the daily physical activity level by the basal metabolic rate from the Schofield's predictive equation) and the mean daily energy intakes (obtained through the Italian food database of the European Institute of Oncology).Fifty-five children (49% F, 51% M, 8–10 y) correctly completed the study. In both periods, the mean BMI corresponded to normal weight status, without significant differences between genders. Energy intake did not change significantly between the assessment periods or between sexes. As expected, the minutes of inactivity decreased during the summer camp period. Moreover, males were more active than females, but the increase in the total energy expenditure from school to summer camp, related to the increased physical activity, was significant in both sexes. Energy balance was negative in both assessment periods and it changed significantly from school to summer camp days, decreasing in both sexes but being more negative for boys than for girls.Our results underline the importance of providing children with meals nutritionally adequate and suggest that the energy content of children's meals should be increased during particular conditions, like a summer camp, and continuously monitored to assure an adequate energy intake to cover the energy requirements during both school and summer camp days.
Changes in ‘liking’ and ‘wanting’ for high fat, high sugar foods and impact on 24hr dietary intake following gastric bypass
Authors: Redpath Tamsyn , Price Ruth , Finlayson Graham , Boyd Adele , Naseer Fathimath , le Roux Carel , Livingstone Barbara 
Published: June 2020

AbstractGastric bypass surgery (GB) is a successful treatment for obesity(1). Following the procedure, patients report changes in preference away from palatable high fat (HF), high sugar (HS) foods that may contribute to weight loss(2). The aim of this work is to examine the relationship between changes in reported ‘liking’ (hedonic) and ‘wanting’(motivation) for HF, HS food and 24hr dietary intake post-GB. 15 GB patients (BMI: 41.7 ± 11.54kg/m2, 73% female) and 15 time-matched controls (BMI: 25.08 ± 4.37 kg/m2, 73% female) were recruited as part of a larger residential study and observed for 3 days at each of 3 time points; 1-month pre-surgery, 3-months post-surgery and 1 yr post-surgery. Covert, objective assessment of 24hr dietary intake was on day 2 of each visit using weighed food records. Participants had ad-libitum access to foods pre-determined by food preference questionnaires and proportionally represented by 6 macronutrient groups (HF/HS, HF/high CHO, HF/high protein, low fat/HS, low fat/high CHO, low fat/high protein). ‘Liking’ and ‘wanting’ was measured using the Leeds Food Preference Questionnaire (LFPQ)(3), a validated measure that uses visual analogue scales to determine explicit liking and wanting for food. Implicit preferences are determined using response and reaction time in a forced-choice task. GB patients significantly reduced their overall energy intake (EI) from baseline to three months (-6.9 ± 8.1MJ/d p = > 0.001) and 1 yr post-surgery (-5.3 ± 7.5MJ/d, p = 0.01) compared to controls. At 3mths post-GB, there was no significant relationship between changes in preference for HS food and changes in %EI from sugar (R2 = 0.97, F(3,26) = 0.94 p = 0.44) or from HS foods (R2 = 0.87, F(3,26) = 0.83, p = 0.49). Changes in preferences for HF food at 3mths post-surgery were not significantly related to %EI from fat (R2 = 0.16, F(3,26) = 1.66, p = 0.2) or HF food (R2 = 0.18, F(3,26) = 0.16, p = 0.93). These associations remained at 1 yr post-surgery. In conclusion, previous observations based on self-reported food intake have found changes in preference for HF, HS food that may contribute to weight loss in GB. In contrast, our results show no significant relationship between changes in preference and changes in dietary intake post-surgery. Further research using direct, objective measures of dietary intake is needed to elucidate further changes in dietary intake post-GB.
Gamma-linolenic and pinolenic acids exert anti-inflammatory effects in cultured human endothelial cells through their elongation products
Authors: Baker Ella , Miles Elizabeth , Calder Philip 
Published: June 2020

AbstractIt is recommended that humans consume fatty fish twice a week to increase dietary intake of eicosapentaenoic acid (EPA) and docosapentaenoic acid (DHA) to achieve long-term health benefits. However current stocks of fish are likely insufficient to meet the needs of humans. Plant-derived polyunsaturated fatty acids (PUFAs) gamma-linolenic acid (GLA) and pinolenic acid (PIN) may provide sustainable land-based sources of bioactive fatty acids.Anti-inflammatory effects of GLA and PIN were compared to EPA and DHA in cultured EA.hy926 cells. Cells were treated with PUFAs (10, 25 and 50 μM) for 48 hours prior to stimulation with tumour necrosis factor for 24 hours. Incorporation of PUFA was measured by gas chromatography; inflammatory responses were measured by ELISA and flow cytometry.All fatty acids were incorporated into EA.hy926 cells, after 48 hours, in a dose dependent manner (10 and 50 μM). Pre-treatment with GLA and PIN (50 μM) resulted in significant increases in their elongation products, dihomo-γ-linolenic acid (DGLA) (p < 0.0001) from GLA and eicosatrienoic (ETrA) (p < 0.0001) from PIN.Pre-treatment with GLA, PIN, EPA or DHA (50 μM) had differential effects depending on fatty acid and cytokine examined. Pre-treatment of EA.hy926 cells with both GLA and PIN resulted in a lower concentration of soluble ICAM-1 (p < 0.01); however EPA and DHA showed greater reduction (p < 0.0001). MCP-1 production was significantly lower after treatment with PIN (p < 0.05), again to a lesser extent than EPA and DHA (p < 0.0001). Pre-treatment with EPA and DHA (50 μM) resulted in lower cell surface expression of ICAM-1 (p < 0.001, p < 0.0001), an effect not observed with GLA or PIN.Anti-inflammatory effects of GLA and PIN were possibly due to their elongation products, and therefore silencing of elongase 5 (ELOVL5) was explored. ELOVL5 siRNA significantly inhibited the production of DGLA and ETrA in EA.hy926 cells pre-treated with GLA and PIN (50 μM). Furthermore significant decreases in sICAM-1 and MCP-1 were not seen after pre-treatment with GLA or PIN in ELOVL5 siRNA silenced EA.hy926 cells.Plant PUFAs (GLA and PIN) demonstrate anti-inflammatory effects in this model using endothelial cells, but are less potent than EPA or DHA. Anti-inflammatory effects of GLA and PIN may be due to their elongation metabolites; DGLA and ETrA.
Molecular markers of dietary essential amino acid-deficiency
Authors: Moro Joanna , Khodorova Nadezda , Chaumontet Catherine , Even Patrick , Blais Anne , Pilard Serge , Rutledge Douglas , Bouveresse Delphine , Piedcoq Julien , Delhaye Nicolas , Gaudichon Claire , Tomé Daniel , Azzout-Marniche Dalila 
Published: June 2020

AbstractIntroduction:The quality of dietary protein sources became a particularly sensitive issue in the current debates on a rebalancing between animal and vegetable food sources.The ability of a protein to meet the nutritional requirements of essential amino acid (EAA) is the basis for assessing the quality of protein.The objective of this study was to characterize the impact of lysine- and threonine-deficient gluten-based diets on the metabolism of growing rats and to identify molecular markers of these diets.Materials and Methods:Growing rats were fed for 3 weeks with a threonine-supplemented and 70% lysine-deficient gluten diet; a lysine-supplemented and 47% threonine-deficient gluten diet; a gluten diet supplemented in lysine and threonine to meet all the AA requirements, and a control diet with milk protein to meet all the AA requirements.Body weight and food intake were measured daily. At the end of the experiment, tissues and biological fluids were removed. The body composition was analyzed, gene expression measurements involved in protein and lipid metabolism were performed and the urinary metabolome was analyzed by LC-MS. Statistical analysis was done by variance analysis and metabolome analysis by discriminant analysis of independent components.Results:This EAA deficiency does not modify the food intake. Lysine deficiency induces a decrease in body weight gain, and lean body mass, associated with an increased in proteolysis and a decreased in proteosynthesis, a decreased in bone mineral density, and no effect on lipid metabolism.Threonine deficiency induces a decrease in body weight gain, and liver and skin weight, without changes in protein metabolism, bone mineral density, and lipid metabolism. After approval of the deficiency model, the metabolomic analysis performed on urine samples revealed the presence of specific discriminating molecules of the diets and types of proteins.Discussion:EAA deficiency has an impact on the growth, and bone and protein metabolism of growing rats. These deficiency states have resulted in different metabolome profiles that could lead to the identification of specific molecular markers of protein sources and related to EAA deficiencies.