bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dynamic arterial elastance during experimental endotoxic septic shock
Authors: García Manuel Ignacio Monge , González Pedro Guijo , Orduña Paula Saludes , Romero Manuel Gracia , Cano Anselmo Gil , Messina Antonio , Rhodes Andrew , Cecconi Maurizio 
Published: May 2020

Abstract
Background: Dynamic arterial elastance (Ea dyn ), the ratio between pulse pressure variation (PPV) and stroke volume variation (SVV), has been suggested as a functional parameter that is a surrogate of arterial load. We aimed to determine the effects of endotoxic septic shock and hemodynamic resuscitation on Ea dyn . Results: Experimental randomized study in a university animal research laboratory with 18 New Zealand rabbits. Animals received placebo (SHAM, n=6) or intravenous lipopolysaccharide (LPS, Escherichia coli serotype 055:B5, 1 mg·kg -1 ) with or without (EDX-R, n=6; EDX-NR, n=6) hemodynamic resuscitation (fluid bolus of 20 ml·kg -1 and norepinephrine for restoring mean arterial pressure). Continuous arterial pressure and aortic blood flow measurements were obtained simultaneously. Cardiovascular efficiency was evaluated by the oscillatory power fraction (%Osc: oscillatory work / left ventricular (LV) total work) and the energy efficiency ratio (EER = LV total work/cardiac output). The LPS-induced endotoxic shock produced a hyperdynamic shock with high cardiac output and arterial hypotension. Ea dyn increased in septic animals (from 0.73 to 1.70; p=0.012) and dropped after hemodynamic resuscitation. Ea dyn was related with the %Osc and EER [estimates: -0.101 (-0.137 – -0.064) and -9.494 (-11.964 – -7.024); p<0.001, respectively]. So, the higher the Ea dyn, the better the cardiovascular efficiency (lower %Osc and EER). Conclusions: In this experimental model, Ea dyn increased during endotoxic septic shock and decreased with hemodynamic resuscitation. Sepsis resulted in a reduced oscillatory power fraction and lowered EER, reflecting a better cardiovascular efficiency that was tracked by Ea dyn . Ea dyn could be a potential index of cardiovascular efficiency during septic shock.
Anti Hepatitis C virus drugs show potential drug repositioning for SARS CoV-2 main protease: an in silico study
Authors: Anandamurthy Arthikasree , Varughese Roslin Elsa , Sivaraj Saranya , Dasararaju Gayathri 
Published: May 2020

Abstract
Developing effective and safe vaccines/drugs against SARS CoV-2 may take some time. The urgency of the outbreak has led to the usage of broad spectrum of existing anti-viral drugs, across the globe. Among the existing anti-viral drugs, there is still a challenge in identifying the potent drug or the combination of the drugs for the better treatment and faster recovery of the patients. In silico molecular docking study aids in the process of drug repurposing and we aimed to identify the binding potential of some of the existing anti-viral drugs and their interactions at the active site of SARS CoV-2 main protease. Results from our study revealed that the drugs Simeprevir, Elbasvir, Paritaprevir, Beclabuvir, Dasabuvir, Teleprevir, Velpatasvir, Ombitasvir, Ledipasvir, Boceprevir, Asunapervir, Declatasvir, Sofusbuvir which are used in the treatment of Hepatitis C virus infection may act as potent inhibitors for SARS CoV-2 main protease.
Rapid Genomic Characterization of SARS-CoV-2 Viruses From Clinical Specimens Using Nanopore Sequencing
Authors: Li Jun , Wang Haoqiu , Mao Lingfeng , Yu Hua , Yu Xinfen , Sun Zhou , Qian Xin , Cheng Shi , Chen Shuchang , Chen Junfang , Pan Jingcao , Shi Jueliang , Wang Xuchu 
Published: May 2020

Abstract
The outbreak of the novel SARS-CoV-2 has swiftly spread worldwide. Rapid genome sequencing of the SARS-CoV-2 strains has become a helpful tool for better understanding of virus genomic characteristics and the origin. To obtain the virus whole-genome sequence directly from the clinical specimens, we performed the nanopore sequencing using a modified ARTIC protocol on portable nanopore sequencer, and validated the routine 8 hours workflow and 5 hours rapid pipeline. We had made some optimizations to improve the genome sequencing workflow. The sensitivity of the workflow was also tested by serially diluting RNA from clinical samples. The optimized pipeline was finally applied to obtain the whole genomes from 17 clinical specimens in Hangzhou from January 2020 to March 2020. In the obtained 17 complete genomes of SARS-CoV-2, 12 variations were found and analyzed. The genomic variations and phylogenetic analysis hinted the multiple sources and different transmission pattern during the COVID-19 epidemic in Hangzhou, China. In conclusion, the genomic characteristics and the origin of the virus could be quickly determined by nanopore sequencing following our workflows.
Posttraumatic stress disorder (PTSD), anger and Mental health of school students in Syria after ten years of conflict: a large scale school-based study
Authors: Kakaje Ameer , Zohbi Ragheed Al , Alyousbashi Ayham , Abdelwahed Rawan N. K. , Aldeen Osama Hosam , Alhalabi Mohammad Marwan , Ghareeb Ayham , Latifeh Youssef 
Published: May 2020

Abstract
The Syrian crisis has entered its tenth year with incessant conflicts in many locations across Syria. Much of the population have been impacted including children who suffer the direct and indirect war effects of war. To date there has been no large scale study examining the mental health and academic impacts of the war of children in Syria. This large-scale cross-sectional study aims to evaluate the psychological profile of school students in Damascus Syria examining PTSD, anger and general mental health and examine their relationships with work, smoking and academic performance. The study included 1369 students from seven different schools. Surveys included assessment of mental and physical well-being using a measure of health related quality of life (SF8), Posttraumatic stress disorder (CRIES-13), problematic anger (DAR5) smoking, working habits, social support and academic grades. The study found that more than half of the children suffered from PTSD (53%), problematic anger (62%) with this being associated with poorer SF-8 (quality of life) scores in both mental and physical domains and more time watching TV and Internet. Around 46% declared fair or worse general health and 61% having moderate or severe mental health. War variables had an impact on PTSD, anger, and SF-8, but not an impact on grades. Worse HRQL was associated with war variables and smoking. Social support was negatively correlated with SF-8, PTSD, DAR-5 anger and declarations of close friends being the main source of support. This is the largest study of children is Syria to date that reports on the psychological ramification of war. The study demonstrates the severe impact on children across both mental health and physical health. Although direct war effect could not be observed, the high burden of PTSD and anger distress is a strong reflection of the chronic mental trauma. School students have had to work and face many hurdles in a young age which has left them severely impacted.
B cell clonal expansion and convergent antibody responses to SARS-CoV-2
Authors: Nielsen Sandra C. A. , Yang Fan , Hoh Ramona A. , Jackson Katherine J. L. , Roeltgen Katharina , Lee Ji-Yeun , Rustagi Arjun , Rogers Angela J. , Powell Abigail E. , Kim Peter S. , Wang Taia T. , Pinsky Benjamin , Blish Catherine A. , Boyd Scott D. 
Published: May 2020

Abstract
During virus infection B cells are critical for the production of antibodies and protective immunity. Establishment of a diverse antibody repertoire occurs by rearrangement of germline DNA at the immunoglobulin heavy and light chain loci to encode the membrane-bound form of antibodies, the B cell antigen receptor. Little is known about the B cells and antigen receptors stimulated by the novel human coronavirus SARS-CoV-2. Here we show that the human B cell compartment in patients with diagnostically confirmed SARS-CoV-2 and clinical COVID-19 is rapidly altered with the early recruitment of B cells expressing a limited subset of V genes, and extensive activation of IgG and IgA subclasses without significant somatic mutation. We detect expansion of B cell clones as well as convergent antibodies with highly similar sequences across SARS-CoV-2 patients, highlighting stereotyped naïve responses to this virus. A shared convergent B cell clonotype in SARS-CoV-2 infected patients was previously seen in patients with SARS. These findings offer molecular insights into shared features of human B cell responses to SARS-CoV-2 and other zoonotic spillover coronaviruses.
Availability of workplace policy for prevention of coronavirus disease 2019 and its relationship with personal protection behaviours: A survey of employees
Authors: WANG Kailu , WONG Eliza Lai Yi , HO Kin Fai , CHEUNG Annie Wai Ling , YEOH Eng Kiong , CHAN Emily Ying Yang , WONG Samuel Yeung Shan 
Published: May 2020

Abstract
Background: The evolving pandemic of coronavirus disease 2019 (COVID-19) has become a severe threat to public health, and the workplace presents high risks in terms of spreading the disease. Few studies have focused on the impact of workplace policy on individual behaviours. This study aimed to examine the relationship of workplace guidelines and measures with employees’ behaviours regarding COVID-19 prevention. Methods: A cross-sectional survey using an online questionnaire was conducted to gather employees’ access to workplace guidelines and measures as well as their personal protection behaviours. Statistical associations between these two factors in different occupations were examined using multiple ordinal logistic regressions. Results: A total of 1048 valid questionnaires across five occupational groups were analysed. Manual labourers reported lower availability of workplace guidelines and measures (76.9% vs. 89.9% for all, P = 0.003). Employees with available workplace guidelines and measures performed personal protection behaviours with higher frequency, and this association was more significant among managers/administrators and manual labourers. Conclusions: Awareness about the disease and pandemic among employers and administrators should be promoted, and resources should be allocated to publish guidelines and implement measures in the workplace. Manual labourers may require specific attention regarding accessibility of relevant information, given their poorer experience of workplace policy and their work nature. Governments should guide the establishment of appropriate policies and responses at the workplace level. Further studies are needed to test the effectiveness of specific workplace policies on COVID-19 prevention.
Two case&nbsp;reports of COVID-19: A 6-year-old boy and his 66-year-old grandmother
Authors: Yi Wanwan , Fan Hengwei , Lv Zhong Wei , Long Xuan , zhu hengmei 
Published: May 2020

Abstract
Background: Currently, COVID-19 is a global pandemic disease that has caused a large number of infections worldwide. Reports of COVID-19 cases are not uncommon, but there is no report that SAMS-CoV-2 RNA test results for grandma and grandchildren in family aggregation infections are completely different.Case presentation: A 6-year-old boy's chest CT indicated lung infection, but SAMS-CoV-2 RNA test was negative. His grandmother's imaging showed lung inflammation absorption, and nucleic acid test was positive. Conclusions: Children and the elderly people have different clinical manifestations during the course of SAMS-CoV-2 infection, it is challenging to identify whether they are infectious. Therefore, the diagnosis of COVID-19 requires a combination of multiple detection measures, including clinical features, imaging features, and SAMS-CoV-2 RNA test results.Keywords: COVID-19; SAMS-CoV-2 RNA test; Imaging diagnosis; Case report
Strategi Komunikasi Dakwah Wilayatul Hisbah dalam Pencegahan Pelanggaran Qanun Aceh tentang Jinayat di Aceh Barat
Authors: Nurjanah Nurjanah 
Published: May 2020

<p><em>Wilayatul Hisbah responded to the high level of violations of the Qanun Jinayat in Aceh. The process of minimizing violations is carried out through the propaganda communication strategy. This article aims to find the communication strategy of preaching Wilayatul Hisbah West Aceh in preventing violations of the Aceh Qanun Number 6 of 2014 concerning Jinayat. The method used is qualitative-descriptive. Data collection techniques carried out by observation, interviews and documentation. The results showed that the Wilayatul Hisbah missionary communication strategy was a communicator strategy, a message strategy, a media strategy, and an effect strategy.</em></p><p>Wilayatul Hisbah merespon tingginya tingkat pelanggaran Qanun Jinayat di Aceh. Proses meminimalisir pelanggaran dilakukan melalui strategi komunikasi dakwah. Artikel ini bertujuan untuk menemukan strategi komunikasi dakwah Wilayatul Hisbah Aceh Barat dalam pencegahan pelanggaran Qanun Aceh Nomor 6 Tahun 2014 tentang <em>Jinayat</em>. Metode yang digunakan adalah kualitatif-deskriptif. Teknik pengumpulan data dilakukan dengan observasi, wawancara dan dokumentasi. Hasil penelitian menunjukkan strategi komunikasi dakwah Wilayatul Hisbah adalah strategi komunikator, strategi pesan, strategi media, dan strategi efek.</p><p><em><br /></em></p>
Reformulasi Metode Dakwah bi al-Lu’bah sebagai Trauma Healing pada Anak Korban Bencana Alam
Authors: Soebahar Moh Erfan , Ghoni Abdul 
Published: May 2020

<p><em>The use of the da'wah bi al lu'bah method can be implemented for children affected by natural disasters. The formulation of the da'wah method can be done by combining the da'wah method with the trauma healing technique. This research was conducted with a literary approach and supported by trauma healing activities at the 2006 earthquake site, namely Klaten District - Central Java and Sleman District - Yogyakarta. In fact researchers did not find the practice of da'wah at these two earthquake locations. The results of the study reveal the formulation of bi al lu'bah propaganda can be done in three ways, namely First, infiltration of propaganda material in the existing game. Infiltrate (infiltration) in the game commonly done by mad'u. Second, making da'wah games, can be done in various ways understood by mad'u children. Third, make poetry about propaganda material through popular songs that can be easily remembered.</em></p><p><em><br /></em></p><p>Penggunaan metode dakwah <em>bi al lu’bah</em> dapat dilaksanakan untuk anak-anak korban bencana alam. Formulasi metode dakwah ini dapat dilakukan dengan penggabungan antara metode dakwah dengan teknik trauma healing. Penelitian ini dilakukan dengan pendekatan literer dan didukung dengan kegiatan trauma healing di lokasi gempa tahun 2006, yaitu Kabupaten Klaten – Jawa Tengah dan Kabupaten Sleman – Yogyakarta. Kenyataannya peneliti tidak menemukan praktik dakwah di dua lokasi gempa ini. Hasil penelitian mengungkapkan formulasi <em>dakwah bi al- lu’bah</em> dapat dilakukan dengan tiga cara, yaitu <em>Pertama,</em> penyusupan materi dakwah pada permainan yang ada. Menyusupkan (infiltrasi) pada permainan yang lazim dilakukan oleh mad’u. <em>Kedua, </em>membuat permainan dakwah, Membuat permainan dakwah dapat dilakukan dengan berbagai cara yang dipahami oleh mad’u anak. <em>Ketiga, </em>membuat syair tentang materi dakwah melalui lagu-lagu populer yang dapat mudah diingat.</p><p><em><br /></em></p>
Dakwah Humanis melaui Gerakan Tarekat
Authors: Moehson Qomariah 
Published: May 2020

<p><em>Humanist propaganda is one way of da'wah with the aim of making humanity. Tarekat is a religious activity which is important for Muslims to draw closer to Allah SWT. The purpose of this study is to analyze the da'wah process through tarekat activities which are considered to have strategic content values in making Muslims better. This study was conducted with a phenomenological qualitative approach. The results of this study reveal that preaching activities through tarekat are good Da'wah choices. The goodness of da'wah through the tarekat is seen as more about the target, where the members of the tarekat are more concerned about their whereabouts, at the same time the members or congregations are also given da'wah material in accordance with their wishes and existence.</em></p><p> </p><p>Dakwah humanis merupakan salah satu cara dalam berdakwah dengan tujuan lebih menjadikan manusia secara lebih manusiawi. Tarekat merupakan sebuah kegiatan keagamaan yang dipandan penting bagi umat Islam dalam mendekatkan diri kepada Allah SWT. Tujuan penelitian ini melakukan analisis terhadap proses dakwah melalui kegiatan tarekat yang dipandang mempunyai nilai kandungan strategis dalam menjadikan umat Islam menjadi lebih baik. Studi ini dilakukan dengan pendekatan kualitatif fenomenologis. Hasil penelitian ini mengungkapkan bahwa kegiatan dakwah melalui tarekat merupakan pilihan dakwah yang baik. Kebaikan dakwah melalui tarekat dipandang lebih mengenai pada sasaran, dimana anggota tarekat lebih diperhatikan keberadaannya, sekaligus para anggota atau jamaah tarekat juga diberi materi dakwah yang sesuai dengan keinginan dan keberadaan dirinya. </p>
Pengaruh E-Servicescape terhadap Trust dan Dampaknya pada Repurchase Intention
Authors: Oebit Zakwannur 
Published: May 2020

Penyedia jasa transportasi online menciptakan aplikasi dengan berbagai fitur demi kenyamanan konsumen. Setiap aplikasi memiliki ciri khas tampilan yang merupakan lingkungan tempat konsumen melakukan transaksi, selanjutnya disebut e-servicescape dalam penelitian ini.
Penelitian ini bertujuan untuk mengetahui seberapa besar pengaruh dari dimensi e-servicescape terhadap kepercayaan dan minat konsumen melakukan pemesanan ulang suatu jasa. Objek penelitian adalah layanan pesan antar makanan pada aplikasi mobile yang bernama GO-FOOD. Sampel penelitian ini adalah 400 responden yang pernah melakukan pembelian produk melalui GO-FOOD di wilayah Jabodetabek yang didapat dengan teknik penarikan sampel purposive sampling.
Variabel yang digunakan dalam penelitian ini didasarkan pada tiga dimensi berdasarkan kerangka e-servicescape, yaitu: aesthetic appeal, layout and functionality, dan financial security. Variabel ini diuji untuk mengidentifikasi apakah variabel tersebut mempengaruhi variabel kepercayaan konsumen (trust) yang mengarah pada variabel minat melakukan pembelian ulang (repurchase intention). Teknik analisis data yang digunakan dalam penelitian ini adalah Covariance Based Structural Equation Modelling (CB-SEM).
Seluruh hipotesis terbukti positif dan didukung dalam penelitian ini. E-servicescape secara signifikan berpengaruh positif terhadap Trust dan selanjutnya secara signifikan berpengaruh positif terhadap repurchase intention. Hasil penelitian ini dapat menjadi rujukan bagi pengembang aplikasi mobile serta bagi penelitian lebih lanjut di bidang yang sama.
E-Supply Chain Management Value Concept for The Palm Oil Industry
Authors: Akbar Muhamad , Darius Antoni 
Published: May 2020

The Harmony of IT and Business is an asset and the foundation of the organizations to improve and build business competitive strategies. There is a need to integrate and coordinate all business partners of the organization, particularly those associated with Information Technology and suppliers, raw materials or resources needed in the supply chain management. The effectiveness of an E-SCM in Palm Oil industry will be the crucial factor to provide the organization with business opportunities and improve competitive position in the marketplace. Aim of this study is to investigates critical factors E -Supply Chain Management in Oil Palm Industry through Theory Resource Based View (RBV). With RBV theory, this study develops a concept that can be utilized for identifying the critical success factors of E-SCM value in Palm Oil industry. The quantitative research method is used for collecting data in four Palm Oil organizations in South Sumatera. This research reveals that IT human resources and IT infrastructure are the critical factors and capability in to operate E-SCM effectively and
Continuous Hypoxia and Glucose Metabolism: The Effects on Gene Expression in Mcf7 Breast Cancer Cell Line
Authors: Al Bawab Abdel Q. , Zihlif Malek , Jarrar Yazan , Sharab Ahmad 
Published: May 2020

Background:
Hypoxia (deprived oxygen in tissues) may induce molecular and genetic
changes in cancer cells.

Objective:
To Investigate the genetic changes of glucose metabolism in breast cancer cell line (MCF7)
after exposure to continuous hypoxia (10 and 20 cycles exposure of 72 hours continuously on a weekly
basis).

Methods:
Gene expression of MCF7 cells was evaluated using real-time polymerase chain reactionarray
method. Furthermore, cell migration and wound healing assays were also applied.

Results:
It was found that 10 episodes of continuous hypoxia activated the Warburg effect in MCF7
cells, via the significant up-regulation of genes involved in glycolysis (ANOVA, p value <0.05). The
molecular changes were associated with the ability of MCF7 cells to divide and migrate. Interestingly,
after 20 episodes of continuous hypoxia, the expression glycolysis mediated genes dropped significantly
(from 30 to 9 folds). This could be attributed to the adaptive ability of cancer cells.

Conclusion:
It is concluded that 10 hypoxic episodes increased the survival rate and aggressiveness of
MCF7 cells and induced the Warburg effect by the up-regulation of the glycolysis mediating gene
expression.
Efektivitas Penggunaan Video Pembelajaran dalam Meningkatkan Motivasi dan Hasil Belajar Siswa Dalam Pembelajaran Front Office di Kelas XI Akomodasi Perhotelan SMKN 3 Garut
Authors: Irwanto Irwanto 
Published: May 2020

<p>Penelitian ini bertujuan untuk mengetahui penggunaan media video terhadap motivasi dan hasil belajar siswa. Pendekatan penelitian yang digunakan adalah pedekatan kuantitatif dengan Metode penelitian Quasi Experimental Design, yaitu desain penelitian eksperimen,dengan tehnik pengumpulan data menggunakan tes dan observasi. Berdasarkan hasil analisis data, diperoleh kesimpulan bahwa penggunaan video pembelajaran sangat efektif untuk meningkatkan motivasi dan hasil belajar siswa dalam pembelajaran Front Office di Kelas XI Akomodasi Perhotelan SMKN 3 Garut. Hal ini ditunjukan dengan adanya perbedaan antara motivasi dan hasil belajar siswa antara kelas yang menerapkan media video dengan kelas yang diberikan media video pembelajaran. </p><p><strong>Kata Kunci</strong>: Video Pembelajaran, Motivasi, Belajar, Front Office</p>