bluuebunny/crossref_metadata_2025_split huggingface dataset:
An Overview of Computational Tools of Nucleic Acid Binding Site Prediction for Site-specific Proteins and Nucleases
Authors: Wan Hua , Li Jian-ming , Ding Huang , Lin Shuo-xin , Tu Shu-qin , Tian Xu-hong , Hu Jian-ping , Chang Shan 
Published: December 2019

:Understanding the interaction mechanism of proteins and nucleic acids is one of the most fundamental problems for genome editing with engineered nucleases. Due to some limitations of experimental investigations, computational methods have played an important role in obtaining the knowledge of protein-nucleic acid interaction. Over the past few years, dozens of computational tools have been used for identification of nucleic acid binding site for site-specific proteins and design of site-specific nucleases because of their significant advantages in genome editing. Here, we review existing widely-used computational tools for target prediction of site-specific proteins as well as off-target prediction of site-specific nucleases. This article provides a list of on-line prediction tools according to their features followed by the description of computational methods used by these tools, which range from various sequence mapping algorithms (like Bowtie, FetchGWI and BLAST) to different machine learning methods (such as Support Vector Machine, hidden Markov models, Random Forest, elastic network and deep neural networks). We also make suggestions on the further development in improving the accuracy of prediction methods. This survey will provide a reference guide for computational biologists working in the field of genome editing.
Design of Carbon-carbon and Carbon-heteroatom Bond Formation Reactions under Green Conditions
Authors: Kerru Nagaraju , Maddila Suresh , Jonnalagadda Sreekantha B. 
Published: December 2019

:The development of C-C and C-heteroatom (C-N, C-O and C-P) bond reactions is a field of significant interest and has received momentous attention in modern organic chemistry. These reactions have been exploited in the synthesis of pharmaceuticals, agrochemicals and molecules of interest in materials science. With the increasing awareness of global warming and the use of renewable energies, it is of paramount importance to reduce the usage of hazardous chemicals in both industrial and academic research and to achieve a healthier environment through green practices. Green chemistry is a rapidly emerging approach that shows us a path for the sustainable growth of future science and technologies. In the recent past, healthy growth has been recorded in a number of organic reactions in aqueous media, which are environment-friendly and energy conserving. This review documents the literature on the development of green methodologies involving the design of C-C, C-O, C-N and C-P bond formations of coupling and condensed reactions. It emphasizes the exceptional practices and important advances achieved using alternative green tools, such as microwave (MW), high-speed ball milling (HSBM) and ultrasound irradiation techniques, and a variety of reusable catalysts and green solvents, with attention to water.
A Case of Small Bowel Obstruction During Labour: Diagnostic and Therapeutic Challenges in a Resource-limited Setting: A Case Report and Review of Management
Authors: Mbonda Aimé Gilbert , Norbert Lemonge , Moutlen Ben Patrick 
Published: December 2019

Background:
Small bowel obstruction (SBO) is defined as the partial or complete interruption
of intestinal content progression in the oral to the aboral sense. It is the third leading cause
of non-obstetrical surgical condition on pregnancy. It is a real emergency that requires early diagnosis
and adequate management to reduce maternal and foetal morbidity and mortality, which remain
high in developing countries.

Case Presentation:
This is the case of a 32-year-old black female refugee. She had a history of
abdominal surgery indicated for splenic rupture during a road accident some 10years ago. She was
admitted to the emergency unit for abdominal pain, vomiting and constipation persistent 4 hours
after home delivery. On admission, the patient was hemodynamically unstable with abdominal distention.
Abdominal ultrasonography revealed dilated loops. After intensive fluid resuscitation, the
patient underwent an emergency laparotomy.

Results:
The findings were a small bowel adhesion obstruction. The postoperative evolution was good.

Conclusion:
Intestinal obstruction on pregnancy is usually diagnosed late. This delays its management
and thus increases the rate of fetal and maternal mortality. The presentation of this case should
appeal to clinicians in emergency rooms (ED) who receive pregnant women to think about nonobstetrical
surgical conditions on pregnancies.
The Effect of Stem Cell Transplantation Therapy for Post Viral Chronic Liver Cell Failure on Associated Type II Diabetes Mellitus: A Pilot Study
Authors: Haydara Tamer , Gabr Mostafa , Abofreikha Mohamed , Bahnasy Abeer , Salama Hosny , Elhendawy Mohammed , Elkadeem Mahmoud , Abd-Elsalam Sherief 
Published: December 2019

Background:
It was observed that type II diabetes mellitus associated with chronic liver
failure improved after stem cell transplantation. However, there were no adequate studies regarding
this issue. The aim of this study was to evaluate the effect of stem cell transplantation on associated
type II diabetes mellitus and on the liver function tests.

Methods:
This pilot study included 30 patients of post-hepatitis chronic liver failure who were classified
into two groups: Group I included patients with chronic liver cell failure associated with type 2
diabetes. Group II included patients without type II diabetes. Autologous CD34+ and CD133+ stem
cells were percutaneously infused into the portal vein. Responders (regarding the improvement of diabetes
as well as improvement of liver condition) and non-responders were determined. Patients were
followed up for one, three and six months after the intervention evaluating their three-hour glucose
tolerance test, C- peptide (Fasting and postprandial), Child-Pugh score and performance score one
month, three months, and six months after stem cell therapy.

Results:
Both synthetic and excretory functions of the liver were improved in 10 patients (66.66 %) of
group I and in 12 patients (80 %) of group II. Significant improvement in the Oral Glucose Tolerance
Test in the responders of both the groups was well defined from the 3rd month and this was comparable
to changes in liver function tests and Child-Pugh score.

Conclusions:
Successful stem cell therapy in chronic liver cell failure patients can improve but not cure
the associating type 2 diabetes by improving insulin resistance.
Coordenação, competição e estabilidade: lições da ‘reforma’ da política de ciência, tecnologia e inovação brasileira
Authors: De Oliveira Joelmo Jesus 
Published: December 2019

Apresentamos uma discussão sobre os esforços de mudança da trajetória da política de ciência, tecnologia e inovação do Brasil por meio da investigação de uma política específica que era a síntese do modelo institucional que se pretendia alterar. A tentativa de mudança foi dirigida para os aspectos normativos da implementação. Conjugamos a análise desses aspectos com o uso de indicadores para o nível de competição e de priorização institucional da alocação orçamentária entre as diferentes macrorregiões do país. Com essa proposta metodológica, conseguimos argumentar sobre as influências de uma provável competição vertical, sobre a formação de capacidades para a coordenação federativa e sobre o papel das instituições e dos atores para a produção tanto de estabilidade quanto de mudança das políticas.Palavras-chave: orçamento público, gestão orçamentária e financeira, investimento, instituição de pesquisa, ciência e tecnologia, inovação, pesquisa científica, bolsa de estudo, administração federal.
Transversalidade de gênero: uma análise sobre os significados mobilizados na estruturação da política para mulheres no Brasil
Authors: Marcondes Mariana Mazzini , Diniz Ana Paula Rodrigues , Farah Marta Ferreira Santos 
Published: December 2019

A criação da Secretaria de Políticas para as Mulheres representou um marco na estruturação da política para mulheres e igualdade de gênero no Brasil. A transversalidade de gênero foi adotada como estratégia de atuação nesse processo, envolvendo um conjunto de instrumentos, organismos, instâncias e mecanismos de gestão e participação social. Neste artigo, investigamos os significados atribuídos à transversalidade, focalizando a estruturação da política para mulheres e igualdade de gênero no Executivo Federal. A partir da análise de documentos, fundamentada no arcabouço teórico proposto por Walby (2005), concluímos que esses significados são indissociáveis das principais questões do feminismo, transbordando a dimensão da gestão governamental. Nesse contexto, os contornos da transversalidade são redefinidos, tanto diante dos desafios de institucionalização da política, quanto pelas disputas e negociações travadas no campo feminista.
Captação de recursos via transferências voluntárias: um olhar para os municípios do Consórcio Intermunicipal da Região Oeste Metropolitana de São Paulo (Cioeste)
Authors: Alvares Merlyn Alvares Ambrosio , Branco Marcello Simão 
Published: December 2019

Este estudo tem o objetivo de analisar a captação de recursos federais, via transferências voluntárias, realizada por municípios que compõem o Consórcio Público Intermunicipal da Região Oeste Metropolitana de São Paulo (CIOESTE). Optou-se por contextualizar o federalismo no Brasil e as atribuições municipais a partir da Constituição Federal de 1988, após identificar o perfil socioeconômico dos municípios que compõem o CIOESTE, para então aprofundar a análise da captação de recursos, utilizando o método de estatística descritiva e análise de cluster. De maneira geral, observa-se que a escolha pelo consorciamento é justificada, uma vez que é uma alternativa cada vez mais buscada por municípios brasileiros para estimular sistemas cooperativos e o desenvolvimento regional. No entanto, alguns municípios do CIOESTE apresentam perfil socioeconômico e comportamento de captação de recursos distintos, o que requer maior atenção da instituição, a fim de melhorar a eficiência e desempenho dos projetos em âmbito municipal e regional.
Desafios da implementação de uma política intersetorial e federativa: as burocracias de médio escalão do Programa Bolsa Família
Authors: Oliveira Vanessa Elias de , Lotta Gabriela Spanghero , Freitas Matheus Nunes 
Published: December 2019

O artigo discute o processo de implementação de uma política pública intersetorial e que demanda coordenação federativa, o Programa Bolsa Família (PBF). Políticas com tais características enfrentam um grande desafio para sua gestão, que é a conexão das distintas burocracias públicas, tanto de diferentes setores quanto de diferentes níveis de governo. Para compreender como estas burocracias percebem os desafios da implementação de uma política com tais características,a pesquisa analisou, por meio de pesquisa qualitativa (entrevistas), a burocracia de médio escalão dos diferentes níveis de governo (municípios, estados e União) envolvida com a implementação do PBF. Demonstramos que, para além das desigualdades locais e das distintas capacidades burocráticas dos níveis de governo, os gestores federais e municipais percebem como um exercício constante a construção da intersetorialidade na operação do programa, e, ainda, entendem que o governo federal deveria desenvolver mecanismos para compreensão do que acontece “na ponta”, nos processos municipais de implementação, incorporando esses aprendizados ao (re)desenho da política, adequando-a às distintas realidades locais.
Inovasi Pembelajaran Tematik, Kendala dan Upaya Perbaikan Dalam Proses Pembelajaran
Authors: Badriah Laelatul , Abibudin Saiful , . Zunita , . Juliyanto 
Published: December 2019

<p>Proses pembelajaran merupakan serangkaian kegaiatan interaksi antara pendidik, peserta didik dan materi ajara yang diorganisasikan sedemikian rupa untuk mencapai tujuan pembelajaran secara maksimal. Dalam kajian ini merupakan potret pembelajaran tematik yang terjadi di salah satu madrasah ibtidaiyah yang berada di wilayah kabupaten Bantul yaitu MIN 1 Bantul, proses pembelajaran tematik terpotret dengan baik dan sangat inovatif, serta sesuai dengan acuan pembelajaran tematik, dalam hal ini tentunya tidak lepas dengan kendala-kendala yang dihadapi oleh setiap guru kelas, akan tetapi kendala tersebut dapat diatasi dengan berbagai upaya diantaranya menggunakan keterampilan dasar mengajar yang inovatif dan fleksibel, metode dan media yang bervariasi dan inoivatif,dan menyusun perencanaan pembelajaran yang lebih flesibel, serta instrumen evaluasi yang sesuai dengan karakteristik materi dan karakteristik peserta didik.</p><p>Kata Kunci: pembelajaran tematik, inovatif</p>
Hidden Communities of Practice in Social Media Groups: Mixed Methods Study
Authors: Skelton Kara , Evans Retta , LaChenaye Jenna 
Published: December 2019

Background
Although most US mothers initiate breastfeeding, suboptimal breastfeeding rates still exist. Although breastfeeding is a complex process, social support has been linked with increases in positive breastfeeding outcomes. Recent technological advances, including the development of social networking sites, provide mothers with convenient access to a unique array of audiences from which to seek advice about parenting, including breastfeeding. However, little is known about how the use of the sites—specifically groups centered around breastfeeding—influences breastfeeding knowledge, attitudes, or behaviors.

Objective
This mixed methods study aimed to explore utilization of an existing probreastfeeding Facebook group and how utilization influences breastfeeding-related knowledge, attitudes, and behaviors.

Methods
Participants were recruited online through Facebook wall posts from within the existing group. Mothers aged between 18 and 50 years who were pregnant and intended to breastfeed, were currently breastfeeding, or had recently weaned their infant in the past 3 years were eligible to participate. Participants engaged in online focus group discussions (n=21) and individual interviews (n=12). Inductive content analysis of qualitative data led to the conceptualization and contextualization of a breastfeeding community of practice (COP). Using qualitative results, a quantitative survey was then developed to assess the prevalence of qualities of a COP as well as how COP usage influenced breastfeeding-related attitudes and knowledge. A total of 314 mothers completed the online survey.

Results
Qualitative findings showed an overall sense of community, with subthemes of group trust, interaction, and the promotion of breastfeeding. A majority (287/314, 91.5%) of mothers initiated breastfeeding, with 69.0% (216/314) of mothers reporting exclusive breastfeeding their infant at 6 months. Approximately 98.5% (309/314) of mothers reported that the Facebook group captured and stored knowledge; therefore, information could be easily accessed and applied. In addition, 96.2% (302/317) of mothers reported that the Facebook group motivated them to share breastfeeding-related knowledge.

Conclusions
The results suggest that this existing probreastfeeding Facebook group exhibits characteristics of an online COP, which was organically formed. Utilization of the Facebook group, in the context of an online COP, could be beneficial in impacting breastfeeding-related knowledge, attitudes, and behaviors. However, further examination and exploration of breastfeeding COPs, including using this type of model as a method of lactation support or as a telemedicine framework, is a clear need.
QardioArm Upper Arm Blood Pressure Monitor Against Omron M3 Upper Arm Blood Pressure Monitor in Patients With Chronic Kidney Disease: A Validation Study According to the European Society of Hypertension International Protocol Revision 2010
Authors: Mazoteras-Pardo Victoria , Becerro-De-Bengoa-Vallejo Ricardo , Losa-Iglesias Marta Elena , López-López Daniel , Rodríguez-Sanz David , Casado-Hernández Israel , Calvo-Lobo Cesar , Palomo-López Patricia 
Published: December 2019

Background
Hypertension is considered as a main risk factor for chronic kidney disease development and progression. Thus, the control and evaluation of this disease with new software and devices are especially important in patients who suffer from chronic kidney disease.

Objective
This study aimed to validate the QardioArm mobile device, which is used for blood pressure (BP) self-measurement in patients who suffer from chronic kidney disease, by following the European Society of Hypertension International Protocol 2 (ESH-IP2) guidelines.

Methods
A validation study was carried out by following the ESH-IP2 guidelines. A sample of 33 patients with chronic kidney disease self-measured their BP by using the QardioArm and Omron M3 Intellisense devices. Heart rate (HR), diastolic BP, and systolic BP were measured.

Results
The QardioArm fulfilled the ESH-IP2 validation criteria in patients who suffered from chronic kidney disease.

Conclusions
Thus, this study is considered as the first validation using a wireless upper arm oscillometric device connected to an app to measure BP and HR meeting the ESH-IP2 requirements in patients who suffer from chronic kidney disease. New validation studies following the ESH-IP2 guidelines should be carried out using different BP devices in patients with specific diseases.
Use of a Smart Watch for Early Detection of Paroxysmal Atrial Fibrillation: Validation Study
Authors: Inui Tomohiko , Kohno Hiroki , Kawasaki Yohei , Matsuura Kaoru , Ueda Hideki , Tamura Yusaku , Watanabe Michiko , Inage Yuichi , Yakita Yasunori , Wakabayashi Yutaka , Matsumiya Goro 
Published: December 2019

Background
Wearable devices with photoplethysmography (PPG) technology can be useful for detecting paroxysmal atrial fibrillation (AF), which often goes uncaptured despite being a leading cause of stroke.

Objective
This study is the first part of a 2-phase study that aimed at developing a method for immediate detection of paroxysmal AF using PPG-integrated wearable devices. In this study, the diagnostic performance of 2 major smart watches, Apple Watch Series 3 and Fitbit (FBT) Charge HR Wireless Activity Wristband, each equipped with a PPG sensor, was compared, and the pulse rate data outputted from those devices were analyzed for precision and accuracy in reference to the heart rate data from electrocardiography (ECG) during AF.

Methods
A total of 40 subjects from patients who underwent cardiac surgery at a single center between September 2017 and March 2018 were monitored for postoperative AF using telemetric ECG and PPG devices. AF was diagnosed using a 12-lead ECG by qualified physicians. Each subject was given a pair of smart watches, Apple Watch and FBT, for simultaneous pulse rate monitoring. The heart rate of all subjects was also recorded on the telemetry system. Time series pulse rate trends and heart rate trends were created and analyzed for trend pattern similarities. Those trend data were then used to determine the accuracy of PPG-based pulse rate measurements in reference to ECG-based heart rate measurements during AF.

Results
Of the 20 AF events in group FBT, 6 (30%) showed a moderate or higher correlation (cross-correlation function>0.40) between pulse rate trend patterns and heart rate trend patterns. Of the 16 AF events in group Apple Watch (workout [W] mode), 12 (75%) showed a moderate or higher correlation between the 2 trend patterns. Linear regression analyses also showed a significant correlation between the pulse rates and the heart rates during AF in the subjects with Apple Watch. This correlation was not observed with FBT. The regression formula for Apple Watch W mode and FBT was X=14.203 + 0.841Y and X=58.225 + 0.228Y, respectively (where X denotes the mean of all average pulse rates during AF and Y denotes the mean of all corresponding average heart rates during AF), and the coefficient of determination (R2) was 0.685 and 0.057, respectively (P<.001 and .29, respectively).

Conclusions
In this validation study, the detection precision of AF and measurement accuracy during AF were both better with Apple Watch W mode than with FBT.
La ciudad, sus guardianes y la justicia. Un estudio de su relación durante la vida republicana de la ciudad de México (1824-1846)
Authors: Flores Flores Graciela 
Published: December 2019

<p>En 1782 se mandó dividir la ciudad de México en ocho cuarteles mayores y 32 menores con ánimo de facilitar el camino de la justicia. A la par, se crearon distintos personajes para cuidar del orden. El presente artículo tiene por principal finalidad conocer a los agentes de seguridad y vigilancia (auxiliares de cuartel, “guardafaroleros”, cabos y vigilantes nocturnos, etcétera) de la ciudad de México, sus funciones y la organización que asumieron para desempeñarlas, además de señalar la estrecha relación que mantuvieron con la justicia durante las dos primeras repúblicas (1824-1846). A través de la cartografía, podremos conocer cómo distribuyeron sus rondas y patrullas para poder dimensionar qué tan efectiva fue su labor al perseguir, aprehender y consignar a los alteradores del orden público ante el juez.</p>
Students’ perceptions concerning the learning environment based on biology teachers’ TPACK
Authors: Nurina Cut Intan Evtia , Riandi R. , Widodo Ari , Yulisman Hendra 
Published: December 2019

Research about students’ perceptions concerning the learning environment based on biology teachers’ TPACK still limited. The purpose of this study was to obtain information about the impact of biology teachers’ TPACK on students’ perceptions concerning their learning environment. TPACK scores were obtained from instrument in the form of multiple-choice test questions. Furthermore, students’ perceptions of their learning environment were obtained using a Likert scale questionnaire. Two biology teachers were used as research samples determined by purposive sampling, which is referring to their TPACK score. Furthermore, students who became the research sample were students from those two teachers that consists of 64 students. The analysis results show that teachers have different TPACK ability at the level of TPACK forming components. Furthermore, there are differences in students' perceptions based on their teachers' TPACK ability. The results were indicated teacher professionalism, in this case, TPACK has the impact on learning environment. Therefore, teachers, schools, government, and education providers are expected to give special attention to teacher professionalism.
RQA integrated with ADI: Empowering biology students’ ability in posing higher-order thinking skills questions
Authors: Amin Astuti Muh , Aloysius Duran Corebima , Zubaidah Siti , Mahanal Susriyati 
Published: December 2019

Questions serve as an element that can be used to access and stimulate students’ thinking ability. This research aimed at analyzing the students’ ability to pose Higher-Order Thinking Skills (HOTS) questions during the learning process. This research was a survey research using a descriptive quantitative approach. The samples used in the research were biology education students of UIN Alauddin Makassar and Universitas Muslim Maros, South Sulawesi with the total of 92 students. The instrument used in this research was an observation sheet of questioning skills for the biology pre-service teachers. The results of this research showed that the implementations of RQA, ADI, and RQA integrated with ADI learning strategies were dominated with HOTS questions, while the learning using the conventional learning strategy was dominated with the Lower-Order Thinking Skills (LOTS) questions. The percentages of the HOTS questions in the learning using RQA, ADI, RQA integrated with ADI, and the conventional learning strategy were 60.53%, 55.71%, 64.91%, and 19.35% respectively. This finding indicates that the RQA integrated with ADI strategy contributes the significant impact in stimulating students’ ability in posing HOTS questions in the classroom.