bluuebunny/crossref_metadata_2025_split huggingface dataset:
National Creutzfeldt–jakob Disease Research Biobank, a Novel Approach to the Establishment of the Scientific Platform: Collaboration Between Patient Advocacy Group, Scientists, Regulators and Physicians
Authors: Anane Alice , Pasternak Doron , Reisner Shimon A. , Novack Victor 
Published: December 2024

Abstract

Creutzfeldt–Jakob disease (CJD) is a severe neurodegenerative disorder characterized by the abnormal accumulation of prion proteins. In Israel, a unique epidemiological pattern of CJD has been identified, specifically a genetic form (gCJD) associated with the E200K mutation in the PRNP gene. Investigating rare diseases such as CJD syndrome poses challenges due to their low prevalence, hindering the formation of an adequate patient cohort for comprehensive research and treatment trials. To overcome this limitation, biobanks have emerged as transformative tools for collecting and distributing biological specimens along with corresponding health data. Biobanks offer a solution to the inherent heterogeneity in rare diseases, allowing researchers to access diverse and extensive sample sets, thereby enhancing the understanding of disease nuances toward potential therapy. We introduce a novel collaborative model involving the Negev BioBank (NBB), the Creutzfeldt–Jakob Israel Foundation, the Israeli National BioBank for Research (MIDGAM), and the Israeli Ministry of Health. Each entity contributes unique expertise and resources to establish a comprehensive platform for studying the disease. The goal was to establish a participant pool of 500 individuals, including clinically diagnosed cases, confirmed gCJD carriers, and their first- and second-degree relatives. During the pilot phase of the last year, 250 participants were enrolled, with each family contributing between 1 and 25 participants. This collaborative approach involving communities, scientists, physicians, and regulatory bodies establishes a model applicable across various fields. These synergistic efforts aim to advance research on CJD and potentially serve as a blueprint for studying other rare diseases.
Objectively measured moderate-to-vigorous physical activity does not attenuate prospective weight gain among African-origin adults spanning the epidemiological transition.
Authors: Davies Jessica C. , Choo-Kang Candice , Soepnel Larske , Geffen Hayli , Africa Chad , Mtintsilana Asanda , Bovet Pascal , Viswanathan Bharathi , Bedu-Addo Kweku , Boateng Prince Oti , Apusiga Kingsley , Dei Oscar Akunor , Forrester Terrence E. , Williams Marie , Lambert Estelle V. , Rae Dale E. , Sinyanya Nandipha , Layden Brian T. , Gilbert Jack A. , Ecklu-Mensah Gertrude , Joyce Cara , Luke Amy , Dugas Lara R. 
Published: December 2024

Abstract

Traditional obesity-related public health messaging often includes physical activity (PA) recommendations. However, at the population level, the data are conflicting, especially when comparing different self-reported vs measured techniques across different settings and populations. We measured the association between moderate-to-vigorous intensity PA (MVPA) and prospective weight change across five African-origin populations and the extent to which MVPA attenuated weight change over time. At baseline, 2,500 adults (median age: 37y) were recruited into the Modelling the Epidemiologic Transition Study (METS), from Ghana, South Africa, Jamaica, Seychelles, and US. 2000 participants were followed up 8 years later, with 851 participants having complete 7-day accelerometry to measure MVPA at both time points. Generalised estimating equations were used to explore the longitudinal association between weight and MVPA adjusted for several confounders. The obesity prevalence at baseline was 27.5% which increased to 38.0% at follow-up. Baseline MVPA varied from 7 (IQR: 4, 16) min/day in US women to 52 (IQR: 36, 78) min/day in South African men, and similarly at follow-up ranged from 8 min/day to 41 min/day among the same participant groups. While overall, engaging in higher MVPA levels was associated with a lower body weight, such that every additional 30 min of MVPA equalled a 600g lower body weight (p = 0.04), the interaction between time and MVPA was not statistically significant (p = 0.18). Therefore, regardless of the amount of MVPA at any time point, body weight increased over time. Despite the association between MVPA and weight, our results suggest that objectively measured longitudinal MVPA was not associated with the change in 8-year weight in African-origin adults. Our research confirms that while PA is a critical determinant of cardiovascular health, it alone may not be enough to stem the rising obesity burden.
NEIL3 Promotes the Carcinogenesis of Prostate Cancer by Activating PI3K/Akt/mTOR Signaling
Authors: Zhang Wei , Liu Zihao , Wen Simeng , Shao Yuan , Yang Zhen , Wang Yong 
Published: December 2024

Abstract

Prostate cancer (PCa) is one of the most common cancers worldwide. Nei endonuclease VIII-like 3 (NEIL3) plays important roles in diverse cancers. In this study, we found that NEIL3 was over-expressed in PCa tissues and cell lines. NEIL3 over-expression is associated with worse prognostic outcomes in PCa patients. In vitro, PCa cell proliferation, invasion, and migration could be significantly inhibited with knocking down NEIL3 by inactivating the phosphatidylinositol 3-kinase (PI3K)/AKT/mammalian target of the rapamycin (mTOR) signaling. Besides, based on the results of RNA-sequencing, we found that the protein expression of high-mobility gene group A2 and androgen receptor (AR) were decreased in C4-2 cells treated with siNEIL3. Therefore, this result suggests that NEIL3 regulates the progression of PCa by modulating the PI3K/AKT/mTOR signaling and the expression of AR.
Effect of Different Doses of Ciprofol on Hemodynamics Induced By General Anesthesia in Elderly Diabetic Patients Undergoing Spinal Surgery: A double-blind, randomized, controlled study
Authors: Jiang Xiao-rui , Zhang Lin-zhong , Ji Jia-wei , Jing Yan , Li Mei-ping 
Published: December 2024

Abstract

Objective To investigate the safety and efficacy of different doses ciprofol in induction of general anesthesia in elderly diabetic patients.
Methods Ninety spinal single-level PLIF patients who were planned to undergo elective tracheal intubation under general anesthesia were selected as the subjects.The study subjects were randomly divided into 3 groups. Ciprofol induction dose was 0.2 mg/kg in group A, 0.3 mg/kg in group B and 0.4 mg/kg in group C, The safety and efficacy of general anesthesia induction were compared among the three groups.
Results The incidence of hypotension in group C (46.4%) was significantly higher than that in group A (7.4%) and group B (14.8%) (P < 0.05). AUCMAP of the three groups was higher in group C (176.39±33.83 mmHg.min) than in group B (158.44±26.55 mmHg.min) and higher in group B than in group A (143.59±19.52 mmHg.min), P < 0.05. The incidence of intubation reaction in group A (77.8%) was the highest (P < 0.05). There were significant differences in BIS values at 3, 4 and 5 minutes after administration.
Conclusion The use of 0.3mg/kg ciprofol in the induction period in elderly diabetic patients has more stable circulation and less vasoactive drug dosage during induction, which is better than 0.2 and 0.4mg/kg dosage groups. The trial was registered at Chinese ClinicalTrials.gov in February 26, 2024 (Registration number:ChiCTR2400081164).
An Analytical Approach for Determining Macro- and Micro- Elements in Two Different Growing Periods of Artemisia abrotanum L
Authors: Cansever İslam , Sogut Ozlem 
Published: December 2024

Abstract

The widespread use of medicinal plants has led to the cultivation of Artemisia abrotanum L., which grows wild in Turkey. The amount of trace elements contained in plants is important for health therefore,analyses of microwave and infusion extracts of the plant were performed using ICP-MS. The evaluation of the elemental content of the harvest time of Artemisia abrotanum L. using a validated method constitutes the aim of the study. Na, K, Mg, Fe, P, S, Ca, Al, V, Cr, Mn, Co, Ni, Cu, Zn, As, Se, Sr, Mo, Cd, Ba, Tl, Li, U were determined. It was observed that the element distribution varied depending on the element in the pre-flowering and flowering periods of the plant. In both extraction methods, Na, Mn, Ni, Co, As, Se, Sr, and Ba concentrations were higher in the blooming period and K, Mg, Ca, Zn, Mo, Cd, and Pb concentrations were higher in the pre-blooming period of Artemisia abratonum L. The amounts of P, Fe, Al, V, Cr, and Tl concentrations differed according to the extraction method. Cu and S concentrations were determined to be similar in both extraction methods and at both harvest times. The results were classified by chemometric analysis and the preparation method and elemental relationships were evaluated. When evaluating the amounts of essential, beneficial, and harmful elements present in Artemisia abrotanum L., consumed as an infusion by the public, independent of harvest time, we found that the detected levels of these elements exceeded the required or harmful thresholds for human health.
Clinical Correlation of CD39 Expression on Killer T Cells in peripheral blood in Cervical Squamous Cell Carcinoma
Authors: LI MENG , Jin Hao , Han Dongmei 
Published: December 2024

Abstract

Aim
To study the correlation between CD39 expression level of CD3 + CD8 + T cells in peripheral blood of patients with cervical squamous cell carcinoma (CESC) and the clinical information.
Methods
This study enrolled 80 participants with CESC, their peripheral blood samples were collected, and flow cytometry was used to detect and analyze the number of CD3 + CD8 + T cells and the expression levels of CD39 and cytokines (IFN-γ and TNF-α) in peripheral blood. Clinical information of tumor patients was collected, and the correlation between the expression levels and clinical characteristics was analyzed and preliminary mechanism exploration was carried out.
Results
The study found that the expression level of CD39 molecule on the surface of CD3 + CD8 + T cells in the peripheral blood of patients with CESC was correlated with the patients' FIGO stage, lymph node metastasis and distant metastasis; IFN-γand TNF-α secretion levels were correlated. This study found that the proportion of CD8 + CD39 + T cells in patients without lymph node and distant metastases was higher than that in patients with lymph node and distant metastases (P < 0.05). This study also found that CESC patients with a higher percentage of CD8 + CD39 + T cells in CD3 + T cells could secrete more IFN-γand TNF-α (P < 0.0001).
Conclusion
In this study, bioinformatics analysis of CESC patients and the detection of the expression level of CD39 molecule on the surface of CD3 + CD8 + T cells in peripheral blood showed that the expression level of CD39 molecule in CESC is related to disease progression. Further studies have found that CD8 + CD39 + T cell subsets have anti-tumor properties and better prognosis for patients.
Effect of Music Therapy on Emotional Resilience, Well-Being, and Employability: A Quantitative Investigation of Mediation and Moderation
Authors: Yingjie Feng , Mingda Wang 
Published: December 2024

Abstract

This study explores the impact of music therapy on emotional resilience, well-being, and employability. Through an 8-week music therapy intervention involving 256 participants, the results demonstrated that music therapy significantly enhanced participants' emotional resilience, which in turn improved their well-being and employability. A significant positive correlation was found between emotional resilience, well-being, and employability, with well-being mediating the relationship between emotional resilience and employability. Additionally, the study identified moderating effects of age and education level, revealing that younger individuals and those with higher education levels benefit more from emotional resilience in terms of well-being and employability. These findings suggest that music therapy is an effective intervention for enhancing emotional resilience and career development, with well-being playing a crucial role in this process. Future research should continue to investigate the long-term benefits of music therapy, explore its effects across diverse populations, and incorporate objective measures and longitudinal designs to validate and expand upon these findings.
Leadless pacemaker implantation after delayed atrial lead perforation and pacemaker energy exhaustion: A case report
Authors: Zhao Yichang , Su Liping , Gao Yuchen , Wang Hao , Luan Chao , Liu Jinqiu , Chen Feifei 
Published: December 2024

Abstract

Background
Delayed lead perforation is a rare complication of a cardiac implantable electronic device (CIED). Clinical presentations range from completely asymptomatic to pericardial tamponade. Surgical lead extraction is recommended and percutaneous lead extraction with surgical backup is an alternative method.
Case presentation
A male with a history of paroxysmal atrial fibrillation and sick sinus syndrome implanted a dual-chamber pacemaker with two passive fixation lead. He was on oral anticoagulants and played golf for almost 1 hour every day after implantation. However, he complained of thoracic stabbing in the sternal manubrium with abnormal findings on pacemaker interrogation. Imaging confirmed the perforated atrial electrode with lead tip protrusion from the pericardium adjacent to the inferior wall of the main right pulmonary artery, but with no pericardial effusion. A lead removal by transvenous approach with surgical support was suggested, but the patient refused. Given the stable condition of the patient, conservative treatment was chosen in the absence of complications during a follow-up period of 14 years. Then pacemaker energy was exhausted with a ventricular lead threshold increased. A leadless pacemaker was implanted.
Conclusions
Chest pain in CIED with abnormal electrical parameters, especially ongoing treatment with anticoagulants and regular physical activity, should always raise suspicion of lead perforation. A conservative strategy may be appropriate and feasible for those in the absence of perforation-related complications. For patients with pacemaker energy exhaustion combined with ventricular lead aging after conservative treatment of lead perforation, leadless pacemaker may be an alternative approach.
Plasma cfDNA VILL gene methylation as a diagnostic marker for nasopharyngeal carcinoma
Authors: Fu Xiao-Yan , Zhou Zi-Ying , Yang Teng-Yue , Wen Ying-Juan , Liu Da-Bo , Zhou Yi-Bo , Yuan Yue , Ye Fei , Huang Zhong-Xi 
Published: December 2024

Abstract

Currently, the non-invasive diagnostic methods for nasopharyngeal carcinoma (NPC) continue to grapple with the challenge of low sensitivity. The hypermethylation of tumor suppressor genes is an established early event in NPC pathogenesis. Consequently, we conducted whole-genome methylation sequencing on plasma cell-free DNA (cfDNA) from six NPC cases and four healthy controls, integrating Illumina Human Methylation 450K microarray data from the GEO database comprising six NPC cases and six samples of non-cancerous nasopharyngeal tissue (NP). As result, we screened only one CpG island associated with cell type-specific regulation within the tumor suppressor gene VILL, which exhibits specific methylation patterns in NPC. We validated our findings using 25 pairs of NPC and NP samples from GEO, alongside 9,736 pan-cancer tissues from TCGA and 656 healthy human leukocyte samples sourced from GEO through methylation microarray analysis. Based on this, we designed a methylation-specific qPCR (qMSP) system for the VILL gene, and then tested it on 192 primary NPC and 154 NC plasma samples. The new qMSP system when compared with EBV DNA qPCR revealed a sensitivity for primary NPC of 80.2% vs.81.3% (78.8% vs.54.5% for early-stage NPC), and a specificity of 100% vs. 93.5%. Notably, employing a combined methodology further enhanced sensitivity to 94.8%, including a sensitivity rate of 90.9% for early-stage NPC diagnosis. Therefore, VILL methylation assessment combined with EBV DNA detection presents a promising avenue for non-invasive diagnosis of NPC, particularly beneficial for early detection.
Does Gender Matter? A Qualitative Analysis of Teaching Award Nominations of General Surgery Chief Residents By Medical Students
Authors: McDermott Colleen E , Stonehocker Dellene , Sun Ting , Lambert Laura 
Published: December 2024

Abstract

Purpose
Gender disparities have been identified in surgical resident teaching award distribution. Qualitative analysis of resident and faculty teaching award nominations has been performed previously; however, qualitative analysis of students' nominations of residents for teaching awards by gender has not yet been explored.
Methods
Nominations for resident teaching awards from the past four years at a single institution were grouped by resident gender. Nominations were then analyzed for thematic content via inductive coding methods using MAXQDA software.
Results
All residents identified as male or female. 40 nominations (13 male, 17 female) were analyzed. The following themes emerged: 1 )Inclusivity 2) Commitment to Teaching 3) Teaching Efficacy 4)Approval of Resident/Role Modeling. Subcategories of major themes were also identified. Many categories were similar for all residents. Notable areas that differed by > 5% of coded text included: female residents were more likely to be described as kind or approachable, and were more commonly described as teaching a specific skill. Male residents were more likely to be described as incorporating the med student into the team, and were more commonly mentioned for teaching in the OR.
Conclusion
This analysis of medical student nominations for resident teaching awards shows areas of thematic difference between male and female surgical residents and provides insight into how learners may assess teaching skills differently by gender. Further study into how this may apply to faculty and impact the learning environment is warranted.
mBAT: A Newly Developed Mobile Application for Self-Screening of Pediatric Bleeding Disorders – A Multi-Center Study
Authors: Sermcheep Arpatsorn , Natesirinilkul Rungrote , Komvilaisak Patcharee , Songthawee Natsaruth , Laohverapanich Kamala , Songdej Duantida , Wongwerawattanakoon Pakawan , Kadegasem Praguywan , Chuansumrit Ampaiwan , Sirachainan Nongnuch 
Published: December 2024

Abstract

Background
Bleeding assessment tools (BATs) are used by trained medical personnel for screening bleeding disorders on a one-to-one basis with patients; hence, they are time consuming and limited its use for large population screening
Objectives
To develop, validate, and demonstrate a Thai version mobile application (mBAT) for self-screening of bleeding disorders
Methods
mBAT was developed and validated from the paper-based Thai pediatric bleeding questionnaire (TPBQ). Then, mBAT was applied to 916 subjects from communities and hematology clinics. Those with scores ≥ 3 were consented for further investigation of bleeding disorders.
Results
mBAT was developed for Thai children using simple sentences, video, pictures, and auto-calculation. It showed a high correlation with the TPBQ: r = 0.96 and 0.97 in the PBQ and ISTH scoring systems, respectively (P < 0.01). A score ≥ 3 was considered abnormal. The mBAT was applied to 916 subjects, with a mean (range) age of 10.5 (0.07-18.0) years. The majority of the subjects (97.3%) were from the community; and the rest (2.7%) were from hematology clinics. The results showed 41 subjects had abnormal scores, and 35 of them (16 from the community and 19 from clinics) consented for diagnostic investigation. Eleven subjects (31.4%) were found to have bleeding disorders, including four with VWD, two each with hemophilia A and thrombocytopenia, and one each with factor VII deficiency, platelet dysfunction, and MYH9-related disorder. Thus, the prevalence rates of bleeding disorders in the subjects whose score ≥ 3 from community and hematology clinics were 6.25% and 52.6%, respectively.
Conclusion
The mBAT had a high correlation with the TPBQ. As a self-screening tool, it could enhance the diagnosis of bleeding disorders.
ANKRD22 Participates in Pro-inflammatory Activities of Macrophages in the Tumor Microenvironment of Colon Cancer
Authors: Wang Xiaoying , Yang Keqing , Yang Bin , Wang Rui , zhu yongliang , Pan Tianhui 
Published: December 2024

Abstract

Tumor-associated macrophages (TAMs) are among the most common types of immune cells in the colon cancer microenvironment. Reprogramming M2-type TAMs with immunosuppressive functions into M1-type TAMs with pro-inflammatory functions is a novel strategy for reshaping the tumor microenvironment (TME) and enhancing the efficacy of immunotherapy in colon cancer. However, the key molecules and mechanisms underlying TAM polarization require further clarification. Our previous study suggested that ANKRD22 may play a role in regulating the functional state transition of macrophages. However, the expression levels of ANKRD22 in colon TAMs and its specific effects on tumor proliferation remain unclear. In the current study, we observed elevated ANKRD22 expression in M1-type TAMs. The expression level of ANKRD22 correlated positively with the survival period of patients with colon cancer, as well as with the infiltration abundance of M1-type TAMs and negatively with the infiltration abundance of M2-type TAMs. A significant decrease in ANKRD22 expression in macrophages co-cultured with colon cancer cell culture supernatant as well as in macrophages directly derived from colorectal cancer tissues were observed. Single-cell RNA sequencing, spatial transcriptomic studies, and subcutaneous xenograft experiments in mice showed that silencing of Ankrd22 altered the subtype distribution of macrophages, attenuated their pro-inflammatory activity, and enhanced their pro-tumor activity. Finally, we identified a potential ANKRD22 agonistic small-molecule lead compound that could contribute to the development of novel therapeutics based on TAM remodeling.
An effective heuristic for developing hybrid feature selection in high dimensional and low sample size datasets
Authors: Shin Hyunseok , Oh Sejong 
Published: December 2024

Abstract

Background.
High-dimensional datasets with low sample sizes (HDLSS) are pivotal in the fields of biology and bioinformatics. One of core objective of HDLSS is to select most informative features and discarding redundant or irrelevant features. This is particularly crucial in bioinformatics, where accurate feature (gene) selection can lead to breakthroughs in drug development and provide insights into disease diagnostics. Despite its importance, identifying optimal features is still a significant challenge in HDLSS.
Results.
To address this challenge, we propose an effective feature selection method that combines gradual permutation filtering with a heuristic tribrid search strategy, specifically tailored for HDLSS contexts. The proposed method considers inter-feature interactions and leverages feature rankings during the search process. In addition, a new performance metric for the HDLSS that evaluates both the number and quality of selected features is suggested. Through the comparison of the benchmark dataset with existing methods, the proposed method reduced the average number of selected features from 37.8 to 5.5 and improved the performance of the prediction model, based on the selected features, from 0.855 to 0.927.
Conclusions.
The proposed method effectively selects a small number of important features and achieves high prediction performance.