bluuebunny/crossref_metadata_2025_split huggingface dataset:
LncRNA LINC00460 promotes the papillary thyroid cancer progression by regulating the LINC00460/miR-485-5p/Raf1 axis
Authors: Li Guangjun , Kong Qingli 
Published: December 2019

Abstract
Background
Papillary thyroid cancer (PTC) is the most common malignancy of all thyroid cancers. LncRNA LINC00460 has been proved to play roles in the oncogenesis and progression of various tumors, including papillary thyroid cancer. However, the potential molecular mechanism of LINC00460 in PTC is poorly investigated.

Results
LINC00460 was upregulated in PTC tissues and cells. Raf1 was upregulated in PTC tissues, but miR-485-5p was down-regulated. High LINC00460 expression was associated with poor prognosis. LINC00460 knockdown suppressed proliferation, migration, invation and EMT of PTC cells. Bioinformatics prediction revealed that LINC00460 had binding sites with miR-485-5p, which was validated by luciferase reporter assay. In addition, miR-485-5p was confirmed to directly target Raf1 3′-UTR. Moreover, LINC00460 promoted PTC progression by sponging miR-485-5p to elevate the expression of Raf1. Knockdown of LINC00460 restrained tumor growth in vivo.

Conclusion
LINC00460 induced proliferation, migration, invation and EMT of PTC cells by regulating the LINC00460/miR-485-5p/Raf1 axis, which indicated that LINC00460 may be a potential biomarker and therapeutic target for PTC.
Successful treatment of hepatic lymphorrhea by percutaneous transhepatic lymphangiography followed by sclerotherapy using OK-432
Authors: Kojima Masayuki , Inoue Masanori , Yamamoto Seiichiro , Kanai Toshio , Nakatsuka Seishi , Nakagawa Motohito 
Published: December 2019

Abstract
Background
Conventional lymphangiography cannot detect leakage sites of hepatic lymphatic vessels. Percutaneous transhepatic lymphangiography can be used to visualize leakage sites, and once the leakage site has been confirmed, effective sclerotherapy can be performed.

Case presentation
A rare case of intractable hepatic lymphorrhea due to injury of the hepatoduodenal ligament following pancreaticoduodenectomy is reported. Drainage of massive ascites from the drainage tube continued after surgery. Percutaneous transhepatic lymphangiography visualized the intrahepatic lymphatic vessels and the leakage site at the hepatic hilum. An 8-Fr drainage catheter was inserted adjacent to the leakage point under fluoroscopic computed tomography guidance. Repeated sclerotherapy using intraperitoneal administration of OK-432 (picibanil) through the catheter was performed, which exposed the leakage site, and control of the ascites was finally achieved.

Conclusions
To the best of our knowledge, this is the first successful case of detection of a leakage site using intrahepatic lymphangiography, followed by sclerotherapy using OK-432.
Social validity of a contextual behavioral science-based intervention for retirement education
Authors: Barbosa Leonardo Martins , Murta Sheila Giardini 
Published: December 2019

AbstractThe literature shows that retirement can bring both positive and negative effects. However, there are few tested interventions for preparing workers for this transition and avoiding or minimizing its negative impacts. This paper presents a study with multiple groups that examined the social validity of an intervention for retirement education grounded in contextual behavioral science and acceptance and commitment therapy. Twenty-seven workers aged 29 to 65 divided into three intervention groups participated (group 1,N = 15; group 2,N = 9; group 3,N = 3). According to the participants’ evaluations, the intervention provided socially valid goals, socially acceptable procedures, and socially important effects. However, some improvements are still needed, such as the use of more dynamic methods, better formatted printed material, and increased fidelity between the content’s implementation and the prescribed activities. The positive results indicate that contextual behavioral science may bolster the development of interventions whose components possess evidence for their social validity. The further evaluation of the intervention via a clinical trial study will offer more robust evidence for its effectiveness. It is hoped that by increasing the availability of theory-based interventions in this area, the present study will promote valid strategies to facilitate better adjustment to retirement.
Evaluation of the genetic diversity of fibre quality traits in upland cotton (Gossypium hirsutum L.) inferred from phenotypic variations
Authors: SUN Zhengwen , WANG Xingfen , LIU Zhengwen , GU Qishen , ZHANG Yan , LI Zhikun , KE Huifeng , YANG Jun , WU Jinhua , WU Liqiang , ZHANG Guiyin , MA Zhiying 
Published: December 2019

Abstract
Background
Evaluating phenotypic traits is very important for the selection of elite lines in Gossypium hirsutum L. Cotton breeders are interested in using diverse genotypes in hybridization that can segregate for traits of interested with the possibility of selection and genetic gain. Information on phenotypic and molecular diversity helps the breeders for parental selection.

Methods
In this study, 719 global collections of G. hirsutum L. were evaluated for five fibre-related traits during two consecutive years in eight different environments. A series of phenotypic data for fibre quality traits were obtained and the elite accessions were further screened using principal component analysis and phylogenetic tree construction based on single nucleotide polymorphism markers.

Results
We found that fibre quality traits showed a wide range of variation among the G. hirsutum accessions over 2 years. In general, accessions from outside China tended to have higher fibre length (FL) and fibre strength (FS) than did Chinese accessions. Among different regional accessions in China, North/Northwest accessions tended to have the highest FL, FS and best fibre macronaire. By assessing five fibre quality traits over 2 years with genotypic data, 31 elite germplasms reaching double-thirty quality values (FL ≥ 30 mm and FS ≥ 30 cN·tex− 1) were selected.

Conclusions
This study provided a detailed phenotypic diversity description of a population representing a wide range of upland cotton germplasm. Our findings provide useful information about possible elite fibre quality parents for cotton breeding programs.
Facial expression recognition using three-stage support vector machines
Authors: Dagher Issam , Dahdah Elio , Al Shakik Morshed 
Published: December 2019

AbstractHerein, a three-stage support vector machine (SVM) for facial expression recognition is proposed. The first stage comprises 21 SVMs, which are all the binary combinations of seven expressions. If one expression is dominant, then the first stage will suffice; if two are dominant, then the second stage is used; and, if three are dominant, the third stage is used. These multilevel stages help reduce the possibility of experiencing an error as much as possible. Different image preprocessing stages are used to ensure that the features attained from the face detected have a meaningful and proper contribution to the classification stage. Facial expressions are created as a result of muscle movements on the face. These subtle movements are detected by the histogram-oriented gradient feature, because it is sensitive to the shapes of objects. The features attained are then used to train the three-stage SVM. Two different validation methods were used: the leave-one-out and K-fold tests. Experimental results on three databases (Japanese Female Facial Expression, Extended Cohn-Kanade Dataset, and Radboud Faces Database) show that the proposed system is competitive and has better performance compared with other works.
Breeding Rice lines for physio-functional food through indica ‘Zhaxima’ × japonica ‘Nanjing 46’ haploid technique
Authors: Tian Q. Q. , Li X. , Lu C. M. , Fang X. W. 
Published: December 2019

AbstractResistant starch (RS) encompasses those forms of starch which are not accessible to human digestive enzymes and are fermented in the colons producing short chain fatty acids. The plant materials containing RS are few in the world. In this contribution, the culture ability of callus from anthers of F1 plants from, landraces, ‘Zhaxima’(Oryza sativa var. indica, high-RS rice line with 7.705 ± 0.142, g/100 g) × ‘Nanjing 46’ (Oryza sativa var. japonica, rice variety with RS content (g/100 g) of 0.200 ± 0.001 crosses were studied for obtaining high RS rice plants. The results showed that when M8 basic induction medium was added with 1.5 mg /L 2,4-D、2 mg /LNAA and 0.3 mg /L KT, the inductivity of callus was high as 32.14% for 21 d after pretreatment at 4 °C for 3 d; When MS differentiation basic medium was added with 2 mg /LKT and 3 mg /L ABA, the frequency of regeneration for callus was 50.3% with only a regeneration frequency of 4.55% grown into green seedlings. The RS content in the seeds was between those of the two parents and was partially normally distributed, the highest RS contents of the regenerated plants was as high as 7.66 ± 1.197%. This produced an efficient technology for regenerating stable rice lines with high RS and good eating quality using anthers culture.
Impact of weight loss on plasma ghrelin level, clinical, and metabolic features of obese women with or without polycystic ovary syndrome
Authors: Rashad Nearmeen M. , Saraya Yasser S. , Afifi Samir A. , Ali Ayman E. , Al-sayed Radwa M. 
Published: December 2019

Abstract
Background
Polycystic ovary syndrome (PCOS) is a common reproductive endocrine co-morbidity of obesity. Ghrelin is a peptide which regulates food intake and body weight. The aim of this study was to measure ghrelin levels in obesity and PCOS and to evaluate the impact of weight loss on plasma ghrelin level, metabolic, and phenotypic features of PCOS. This prospective comparative study enrolled obese women without PCOS (N = 60) and obese PCOS women (n = 50) and 85 control groups. Body compositions including fat mass (FM) and fat-free mass (FFM) were measured by dual-energy X-ray absorptiometry (DEXA). Plasma ghrelin concentrations were measured using enzyme-linked immunosorbent assay (ELISA).

Results
Our results revealed that plasma ghrelin levels were lower in PCOS patients compared to obese (9.49 ± 5.59 ng/ml) and controls (48.21 ± 21.09 ng/ml). Moreover, it was negatively correlated to anthropometric measures, glycemic, lipid profile, and the phenotype characteristics of PCOS. Interestingly, after 12 weeks of following the Mediterranean diet (MD)-based weight loss program, ghrelin levels were increased in both obese groups.

Conclusion
Successful weight loss leads to increase ghrelin levels in both obese and PCOS groups.
Medical Management of Patients After Atypical Femur Fractures: a Systematic Review and Recommendations From the European Calcified Tissue Society
Authors: van de Laarschot Denise M , McKenna Malachi J , Abrahamsen Bo , Langdahl Bente , Cohen-Solal Martine , Guañabens Núria , Eastell Richard , Ralston Stuart H , Zillikens M Carola 
Published: December 2019

Abstract

Context
Atypical femur fractures (AFFs) are serious adverse events associated with bisphosphonates and often show poor healing.

Evidence acquisition
We performed a systematic review to evaluate effects of teriparatide, raloxifene, and denosumab on healing and occurrence of AFF.

Evidence synthesis
We retrieved 910 references and reviewed 67 papers, including 31 case reports, 9 retrospective and 3 prospective studies on teriparatide. There were no RCTs. We pooled data on fracture union (n = 98 AFFs on teriparatide) and found that radiological healing occurred within 6 months of teriparatide in 13 of 30 (43%) conservatively managed incomplete AFFs, 9 of 10 (90%) incomplete AFFs with surgical intervention, and 44 of 58 (75%) complete AFFs. In 9 of 30 (30%) nonoperated incomplete AFFs, no union was achieved after 12 months and 4 (13%) fractures became complete on teriparatide. Eight patients had new AFFs during or after teriparatide. AFF on denosumab was reported in 22 patients, including 11 patients treated for bone metastases and 8 without bisphosphonate exposure. Denosumab after AFF was associated with recurrent incomplete AFFs in 1 patient and 2 patients of contralateral complete AFF. Eight patients had used raloxifene before AFF occurred, including 1 bisphosphonate-naïve patient.

Conclusions
There is no evidence-based indication in patients with AFF for teriparatide apart from reducing the risk of typical fragility fractures, although observational data suggest that teriparatide might result in faster healing of surgically treated AFFs. Awaiting further evidence, we formulate recommendations for treatment after an AFF based on expert opinion.
Increased Prevalence of TG and TPO Mutations in Sudanese Children With Congenital Hypothyroidism
Authors: Bruellman Ryan J , Watanabe Yui , Ebrhim Reham S , Creech Matthew K , Abdullah Mohamed A , Dumitrescu Alexandra M , Refetoff Samuel , Weiss Roy E 
Published: December 2019

Abstract

Context
Congenital hypothyroidism (CH) is due to dyshormonogenesis in 10% to 15% of subjects worldwide but accounts for 60% of CH cases in the Sudan.

Objective
To investigate the molecular basis of CH in Sudanese families.

Design
Clinical phenotype reporting and serum thyroid hormone measurements. Deoxyribonucelic acid extraction for whole-exome sequencing and Sanger sequencing.

Setting
University research center.

Patients
Twenty-six Sudanese families with CH.

Intervention
Clinical evaluation, thyroid function tests, genetic sequencing, and analysis. Our samples and information regarding samples from the literature were used to compare TG (thyroglobulin) and TPO (thyroid peroxidase) mutation rates in the Sudanese population with all populations.

Results
Mutations were found in dual-oxidase 1 (DUOX1), dual-oxidase 2 (DUOX2), iodotyrosine deiodinase (IYD), solute-carrier (SLC) 26A4, SLC26A7, SLC5A5, TG, and TPO genes. The molecular basis of the CH in 7 families remains unknown. TG mutations were significantly higher on average in the Sudanese population compared with the average number of TG mutations in other populations (P &lt; 0.05).

Conclusions
All described mutations occur in domains important for protein structure and function, predicting the CH phenotype. Genotype prediction based on phenotype includes low or undetectable thyroglobulin levels for TG gene mutations and markedly higher thyroglobulin levels for TPO mutations. The reasons for higher incidence of TG gene mutations include gene length and possible positive genetic selection due to endemic iodine deficiency.
Verification of a Monte Carlo dose calculation engine in proton minibeam radiotherapy in a passive scattering beamline for preclinical trials
Authors: Guardiola Consuelo , De Marzi Ludovic , Prezado Yolanda 
Published: December 2019

Objectives:
Proton minibeam radiation therapy (pMBRT) is a novel therapeutic strategy that combines the benefits of proton therapy with the remarkable normal tissue preservation observed with the use of submillimetric spatially fractionated beams. This promising technique has been implemented at the Institut Curie-Proton therapy centre (ICPO) using a first prototype of a multislit collimator. The purpose of this work was to develop a Monte Carlo-based dose calculation engine to reliably guide preclinical studies at ICPO.

Methods:
The whole “Y1”-passive beamline at the ICPO, including pMBRT implementation, was modelled using the Monte Carlo GATE v. 7.0 code. A clinically relevant proton energy (100 MeV) was used as starting point. Minibeam generation by means of the brass collimator used in the first experiments was modelled. A virtual source was modelled at the exit of the beamline nozzle and outcomes were compared with dosimetric measurements performed with EBT3 gafchromic films and a diamond detector in water. Dose distributions were recorded in a water phantom and in rat CT images (7-week-old male Fischer rats).

Results:
The dose calculation engine was benchmarked against experimental data and was then used to assess dose distributions in CT images of a rat, resulting from different irradiation configurations used in several experiments. It reduced computational time by an order of magnitude. This allows us to speed up simulations for in vivo trials, where we obtained peak-to-valley dose ratios of 1.20 ± 0.05 and 6.1 ± 0.2 for proton minibeam irradiations targeting the tumour and crossing the rat head. Tumour eradication was observed in the 67 and 22% of the animals treated respectively.

Conclusion:
A Monte Carlo dose calculation engine for pMBRT implementation with mechanical collimation has been developed. This tool can be used to guide and interpret the results of in vivo trials.

Advances in knowledge:
This is the first Monte Carlo dose engine for pMBRT that is being used to guide preclinical trials in a clinical proton therapy centre.
MRI surveillance for women with a personal history of breast cancer: comparison between abbreviated and full diagnostic protocol
Authors: Park Ko Woon , Han Sol Bee , Han Boo-Kyung , Ko Eun Sook , Choi Ji Soo , Rhee Sun Jung , Ko Eun Young 
Published: December 2019

Objective:
To compare the diagnostic performance of breast MRI with abbreviated protocol (AB-MRI) and full ddiagnostic protocol (FDP-MRI) for surveillance of females with a personal history of breast cancer

Methods:
In this retrospective study, we analyzed the outcomes of total 1312 post-operative screening breast MRI matched from 1045 AB-MRI and 677 FDP-MRI, which had histologic confirmation for suspicious MRI findings or 1 year negative follow-up images. This study was approved by the institutional review board and informed patient consent was waved. AB-MRI consists of T 2 weighted scanning and dynamic contrast-enhanced imaging including one pre-contrast and two post-contrast scans. We compared the diagnostic performance for recurrent breast cancer in terms of sensitivity, specificity, positive-predictive value, negative-predictive value, and accuracy and area under the curve between the screening AB-MRI and FDP-MRI.

Results:
Overall, 13 recurrent tumors among 1312 post-operative cases screened with breast MRI (1.0%) were detected including 8 invasive cancer, 2 cases of in situ cancer, and 3 cases of metastatic lymph nodes. The sensitivity and negative predictive value were 70 vs 100 and 99.5% vs 100% in AB-MRI and FDP-MRI. Specificity, positive predictive value, accuracy, and area under the curve of AB-MRI and FDP-MRI were 98.0% vs 96.9%, 35.0% vs 23.1%, 97.6% vs 97.0%, and 0.840 vs 0.985, respectively.

Conclusion:
The performance of AB-MRI was comparable to that of FDP-MRI in detecting recurrent breast cancer and decreased false positive cases.

Advances in knowledge:
AB-MRI provides a reliable alternative with similar diagnostic performance and shorter MRI acquisition time.
Association between thyroid nodule size and malignancy rate
Authors: Jinih M , Faisal F , Abdalla K , Majeed M , Achakzai AA , Heffron C , McCarthy J , Redmond HP 
Published: December 2019

Introduction The diagnostic performance of ultrasound-fine needle aspiration to identify thyroid nodules harbouring malignancy remains variable. The aim of this study was to determine thyroid nodule size and cytological classification as predictors of malignancy risk. Materials and methods We conducted a retrospective cohort analysis at an academic hospital involving 499 consecutive patients who underwent thyroid surgery between 2004 and 2015. Results A total of 503 thyroid nodules (499 patients, 84% female; mean age 50.8 years, standard deviation, SD, 15.4 years) were analysed. Of these, 19.5% were malignant. The mean (± SD) nodule size was 3.28 ± 1.63 cm and 3.27 ± 1.54 cm for benign and malignant nodules, respectively. The odds of malignancy for thyroid nodules less than 3.0 cm was similar to those for nodules of 3.0 cm or greater (0.26 compared with 0.29; p=0.77). Overall, the sensitivity and specificity of fine-needle aspiration in this cohort were 71.4% and 100%, respectively. The overall false negative rate was 5.4%. When the cut-off of 3.0 cm was used, the false negative rate in thyroid nodules less than 3.0 cm was 0% compared with 7.0% in nodules of 3.0 cm or greater. Thus, class (p<0.01) but not nodule size (p=0.49), was associated with higher malignancy risk. Conclusions Our results suggest that thyroid nodule size did not accurately predict the risk of thyroid malignancy irrespective of fine-needle aspiration cytology. Routine diagnostic thyroid lobectomy solely owing to thyroid nodule size of 3.0 cm or greater is currently not justified.
Comparison Performance of the Multi-Regional Climate Model (RCM) in Simulating Rainfall and Air Temperature in Batanghari Watershed
Authors: Handoko Unggul , Boer Rizaldi , Aldrian Edvin , Latifah Arnida L. , Dasanto Bambang D. , Apip Apip , Misnawati Misnawati 
Published: December 2019

Many scientists assume that RCM output is directly used as input for climate change impact models, while it consists of systematic errors. Consequently, RCM still requires bias correction to be used as an input model. The purpose of this study was to analyze the RCM performance before and after bias correction, its best performance from several models, as well as to clarify the importance of bias correction before it is used to analyze climate change. As a result of this, the method used for bias correction was Distribution Mapping method (for rainfall) and Average Ratio-method (for air temperature). While the Generalized Extrem Valuedistribution (GEV) was used to analysis extreme rainfall. To determine the performance of the model before and after bias correction, statistical analysis was used namelyR2, NSE, and RMSE. Furthermore, ranking for every single model and Taylor Diagram was used to determine the best model. The results showed that the RCMs performance improved with bias correction. However, CSIRO-Mk3-6-0, CCSM4, GFDL-ESM2M, and MPI-ESM-MR models can be ignored as ensemble models, because they demonstrated poor performance in simulating rainfall. From this study, it was suggested that the best model in simulating daily and monthly rainfall was ACCESS1-0, while MIROC-ESM-CHEM (daily air temperature) and ACCESS1-0 (monthly air temperature) were best models used in simulating air temperature. Key words: RCM, bias correction, performance, rainfall, air temperature
Design and analysis of refractive index sensors based on slotted photonic crystal nanobeam cavities with sidewall gratings
Authors: Fu Yao-Long , Deng Chao-Sheng , Ma Song-Shan 
Published: December 2019

We propose and numerically investigate a refractive index sensor based on a one-dimensional slotted photonic crystal nanobeam cavity with sidewall gratings for refractive index sensing in a gaseous environment. By using the three-dimensional finite-difference time-domain method, we demonstrate that our proposed sensor simultaneously possesses a high quality factor of

3.71
×

10
6

and a high sensitivity of 508 nm/RIU (refractive index unit) at the resonant wavelength near 1583 nm, yielding a detection limit as low as

1.97
×

10

−
6

RIU. Moreover, the mode volume of the cavity’s fundamental resonant mode is found to be as small as

0.022
(
λ

/

n

)
3

, resulting in a very compact effective sensing area. We finally study and assess the effect of fabrication disorder on the performances of our proposed sensor. We believe our proposed sensor will be a promising candidate for applications not only in multiplexed biochemical sensing and multielement mixture detection, but also in optical trapping of single biomolecules or nanoparticles.