bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Meaning-centered spiritual care training program for hospice palliative care team in South Korea: development and preliminary evaluation
Authors: Kim Shin-Jeong , Kang Kyung-Ah , Kim Do-Bong , Park Myung-Hee , Yoon Soo-Jin , Choi Sung-Eun , Choi Young-Sim , Koh Su-Jin 
Published: September 2020

Abstract
Background
Spirituality is a fundamental and intrinsic aspect of human beings and should be a core component of quality palliative care. There is an urgent need for training of hospice palliative care teams (HPCTs) to enhance their competency to provide spiritual care. The purpose of this study was to develop and evaluate a meaning-centered spiritual care training program for hospice palliative care teams (McSCTP-HPCT).
Methods
In this methodological study we developed a training program for HPCTs, using the ADDIE educational content developmental model. The final training program comprised five modules. The modules’ content was informed by Viktor Frankl’s meaning-centered logotherapy with its emphasis on spiritual resources as well as the spiritual care model of ISPEC (Interprofessional Spiritual Care Education Curriculum). Following development, a pilot test was conducted with four nurses. The results of the pilot were used to inform the final program. The final program was tested in an intervention involving 13 hospice palliative care nurses. Measurements using self-administered questionnaires were taken at three points before and after the intervention. Participants’ demographic and career-related characteristics and the degree of variance between outcome variables (compassion fatigue [CF], spiritual care competency [SCC], and spiritual care therapeutics [SCT]) were analyzed using descriptive statistics, t-test, and one-way ANOVA.
Results
The MCTP-HPCT was developed into five modules. Module I: The HPCT’s SCC evaluation, understanding the major concepts of spiritual care and logotherapy, Module II-IV: Meaning-centered intervention related to the spiritual needs (existential, relational, and transcendental/religious), Module V: The process of meaning-centered spiritual care. The preliminary evaluation showed a significant differences in all three outcome variables at the first measure point (CF, p = 0.037; SCC, p = 0.005; SCT, p = 0.002). At the second measure point statistical significance was found only with SCC (p = 0.006).
Conclusions
The MCTP-HPCT developed in this study is suitable for use in clinical settings and provides evidence for evaluating the spiritual care competency of HPCTs.
The Early Loading of Different Surface Modified Implants: A Prospective Clinical Study
Authors: Körmöczi Kinga , György Komlós , Petra Papócsi , Ferenc Horváth , Árpád Joób-Fancsaly 
Published: September 2020

Abstract
The early loading of different surface treated dental implants is compared in our clinical study. Patients were selected from the outpatient ward of the Department of Oro-Maxillofacial Surgery and Stomatology at the Semmelweis University. The secondary stability, that evolved after 6 weeks of healing, was measured on acid etched/sand blasted (SLA) and bio-hydroxyapatite coated implants. Osstell® and Periotest® were applied to determine this value. Periodontal probing depth appearing after 3 months of loading was checked on 6 points next to the implant supported prosthesis. Shapiro-Wilk and Mann-Whitney tests have been chosen to fulfil the statistical analysis. According to our results, a similar satisfying secondary stability can be obtained independently of the modality of the surface. The values were higher in the bio-hydroxyapatite group; however, this difference was not significant. The outcome of measuring the probing depth reflects the importance of frequent recalls of patients. The necessary ethical approval for our study has been provided by the National Institute of Pharmacy and Nutrition (reference number: OGYÉI/55197/2019 ).
Digital Three-Dimensional Visualization of Intrabony Periodontal Defects for Surgical Planning (Pilot Case Study)
Authors: Palkovics Daniel , Mangano Francesco Guido , Nagy Katalin , Windisch Peter 
Published: September 2020

Abstract
Background: In the regenerative treatment of intrabony periodontal defects, surgical strategies are determined by defect morphologies. Clinical direct measurements and intraoral radiographs are the main tools in periodontal diagnostics and surgical planning, however in certain cases they don’t provide sufficient amount of information. Therefore, the application of cone-beam computed tomography (CBCT) in diagnosis and treatment planning of periodontally involved patients has been proposed. The aim of this study is to present a novel method for 3D visualization of intrabony periodontal defects on digital models reconstructed from CBCT datasets for diagnostics and treatment planning.Methods: 4 patients with a total of 6 intrabony periodontal defects were enrolled in the present study. 2 months following initial periodontal treatment CBCT scan is taken. Radiographic image processing (segmentation) of CBCT datasets were performed in a radiographic imaging software to acquire anatomically accurate, virtual three-dimensional polygon models of surgical areas. Intrasurgical and digital measurements were taken, and results were compared, to validate the accuracy of digital models.Results: Difference between intrasurgical- and digital measurements in depth and width of intrabony components of periodontal defects were 0,31±0,21 mm and 0,41±0,44 mm respectively.Conclusion: It can be concluded that, the described digital workflow is useful in the treatment of certain periodontal intrabony defect morphologies. However, to determine the exact use cases of such technology further studies and examination is necessary.Trial Registration: Retrospective Ethics Approval
Validation and Comparison of [18F]NaF PET/CT Analysis Methods and Derivation of a Semi-population Input Function for Site Specific Measurements of Bone Formation in a Population with Chronic Kidney Disease Mineral and Bone Disorder
Authors: Vrist Marie Houmaa , Bech Jesper Nørgaard , Lauridsen Thomas Guldager , Fynbo Claire Anne , Theil Jørn 
Published: September 2020

Abstract
BackgroundMethods for assessing bone metabolism which can replace the gold standard bone biopsy are sorely needed in the clinical setting in patients with chronic kidney disease - mineral and bone disorder (CKD-MBD). The aim of the present study was implementation, validation and comparison of non-invasive dynamic and static whole-body (WB) [18F]NaF PET/CT scan methods to replace invasive bone biopsy, used for quantitative analysis of bone clearance in CKD-MBD patients.MethodsSeventeen patients with CKD-MBD underwent a 60-minute dynamic scan followed by a 30-minute static WB scan. Tracer kinetics in four thoracic vertebrae were analyzed using non-linear regression with the Hawkins model and two different Patlak-analysis methods using image-derived arterial input functions obtained from the left myocardial ventricle and the thoracic aorta. For future use in WB PET/CT scans, we validated the use of a semi-population input function. The resulting kinetic parameters (Ki, K1, k2, k3, k4, V0) were compared between the different methods of analysis using the various derived input functions.ResultsDynamic kinetic results from both standard and single-point Patlak analyses correlate well with non-linear regression analysis, but Ki results using Patlak analysis are generally lower for all tested input functions compared to regression analysis. Single-point Patlak analysis on WB scans correlates well with standard Patlak analysis on dynamic scans but show 13-21% higher Ki-values. ConclusionsOur results show good correlation between dynamic and static analysis of skeletal plasma clearance but different Ki-values depending on the analysis method and choice of input function used. Thus, WB [18F]NaF PET/CT scans can be applied in future studies, but the results should not be compared uncritically with results obtained using non-linear regression analysis.
Comparative Microsomal Proteomics of A Model Lung Cancer Cell Line NCI-H23 Reveals Distinct Differences Between Molecular Profiles of 3D and 2D Cultured Cells
Authors: Blonder Josip , Kaczmarczyk Jan A. , Roberts Rhonda R. , Luke Brian T. , Chan King C. , Wagoner Carly M. Van , Felder Robin A. , Saul Richard G. , Simona Colantonio 
Published: September 2020

Abstract
Background: Lung cancer is the leading cause of cancer-related deaths in the USA and worldwide. Yet, about 95% of new drug candidates validated in preclinical phase eventually fail in clinical trials. Such a high attrition rate is attributed mostly to the inability of conventional two-dimensionally (2D) cultured cancer cells to mimic native three-dimensional (3D) growth of malignant cells in human tumors. Thus, it is expected that 3D cell culture systems would more accurately represent the phenotype of cancer cells growing in tumors. To ascertain phenotypical differences between these two distinct culture conditions, we carried out a comparative proteomic analysis of membrane fraction obtained from 3D- and 2D-cultured NSCLC model cell line NCI-H23. Methods: Global shotgun membrane (SGM) proteomics that relies on strong cation exchange (SCX)-based peptide fractionation and accurate-mass, liquid chromatography mass spectrometry (HR/AM LC-MS) was employed to analyze microsomal fractions obtained from the NCI-H23 cells grown in both 2D and 3D culture conditions. Results: Comparative proteomics revealed a map of 1,166 (24%) nonredundant protein species regulated in culture dependent manner in NCI-H23 cell line. Of these, a subset of 234 (i.e., 21 %) proteins were found significantly dysregulated (p-value ≤ 0.05) under both culture conditions whereas a total of 334 (27.8%) and 598 (51,2%) proteins were uniquely identified in 3D and 2D culture, respectively. The Ingenuity Pathway Analysis revealed extensive metabolic changes and differential regulation of a subset of CD molecules in culture-dependent manner. Using western blotting we verified exclusive 3D-culture expression of CD99, CD146 and CD239, involved in development of malignant stroma extracellular matrix, neo-angiogenesis and metastasis. Furthermore, using label-free quantitation we unambiguously confirmed upregulation of wild type and monoallelic KRas4B G12C mutant in 3D cultured NCI-H23 cells, targeting exclusively proteoform-specific tryptic peptides. Conclusions: In this study we generated a large-scale proteomic resource/atlas of a preclinical testing model NCI-H23 cell line grown in 3D- and 2D-culture, providing insight into phenotypical/proteomic changes unique to each culture type, that would not have been discovered using only conventional 2D-culture. To reduce high attrition rate of new drug candidates it is critical to profile a large number of patient-derived NSCLC cell lines.
Using Genetics to Inform The Role of Hemostatic Factors in COVID-19: A Mendelian Randomization Study
Authors: Schooling CM , Yeung SL Au , Zhao JV 
Published: September 2020

Abstract
Background: Severe corona virus disease 19 (COVID-19) is challenging to treat as it presents with thrombotic as well as inflammatory features. Specifically, endothelial cells are thought to be damaged in COVID-19. Here we assessed whether key druggable targets related to endothelial function, i.e., nitric oxide and platelets, are affected specifically in severe COVID-19, using a Mendelian randomization study.Methods: We compared genetically predicted circulating nitric oxide, proxied by three relevant NOS3 functional variants (rs2070744, rs1799983 and rs3918226), and genetically predicted platelets, proxied by a functional variant related to platelet reactivity from GRK5 (rs10886430) and by platelet count (based on 243 variants), because they share a phenotype, in people with severe COVID-19 with the general population (cases=536, non-cases=329,391) largely based on studies from Northern Europe. We made a similar comparison for any COVID-19. Results: Nitric oxide was inversely associated with severe COVID-19 (odds ratio (OR) 0.84 per raising allele, 95% confidence interval (CI) 0.75 to 0.95), but was not associated with any COVID-19. Associations of both platelet reactivity and platelet count with severe COVID-19 were in a harmful direction (OR 1.20, 95% CI 0.95 to 1.50 and OR 1.23, 95% CI 0.97 to 1.56 respectively) with combined OR 1.21, 95% CI 1.03 to 1.42, but associations with any COVID-19 were null.Conclusions: Genetic validation of the role of nitric oxide and possibly of platelets in specifically severe COVID-19 suggests they could be targets of intervention for which well-established treatments exist.
Comparison of the Effects of Rapid Maxillary Expansion vs Twin Block Appliance on Mandibular Growth in Skeletal Class II Patients.
Authors: Zhang Jia-Nan , Chen Si , Huang Cheng-Yi , Zhong Chong , Jin Jing , Yu Feng-Yang , Zhang Zan-Zan , Lu Hai-Ping 
Published: September 2020

Abstract
Background: This is a retrospective study that compares mandibular growth changes in skeletal Class II patients treated by rapid maxillary expansion (RME) and following fixed appliance with those patients treated by Twin-Block (TB) and following fixed appliance. Methods: Fourteen patients treated by RME and following fixed appliance were included into the RME group. Fifteen patients treated by Twin-Block and following fixed appliance were included into the TB group. Lateral cephalometric radiographs taken before treatment and immediately after fixed appliance treatment were used to evaluate mandibular growth effects. Results: The starting forms of the patients in the two groups were examined to be of good comparability. The mandibular length increased significantly in both groups as measured by Co-Gn, Go-Gn and Ar-Gn, but the TB group didn’t show more mandibular growth than the RME group (P>0.05). Skeletal changes of the mandible in vertical dimension were different in the two groups. The change in FMA was 0.35° in the RME group, while the change was 2.65° in the TB group (P<0.001). The change in LAFH was 5.14mm in the RME group, significantly smaller than the change of 10.19mm in the TB group (P<0.001).Conclusions: The investigated Phase I treatment with RME followed by Phase II treatment of fixed appliance achieved the same increases in sagittal mandibular growth and facial profile improvements as the Twin-Block therapy. The treatment with RME followed by fixed appliance was better for vertical control, while the treatment with Twin-Block followed by fixed appliance significantly increased the mandibular plane angle.
Level of pain and physical function in patient with chronic knee pain visiting Dhulikhel hospital: A cross sectional study
Authors: Kandel Binaya , Badal Surakshya 
Published: September 2020

Abstract
Background

Knee pain is one of the common causes of chronic pain mainly in the older adults.Chronic knee pain (CKP) is commonly due to arthritic changes (osteoarthritis). Chronic knee pain is a common and major health problem in ageing population. Knee pain is associated with high levels of disability. So early detection and treatment of pain related functional limitation is likely to have major influence on healthy ageing. The general objective of this study was to quantify the level of pain and physical function in patient with CKP.
Methods

Preliminary screening of population with chronic knee pain was taken and cross sectional descriptive study was done. Questionnaire with inclusion criteria was fulfilled with administration of Nepali version of Numerical Pain Rating Scale and Nepali version of Patient Specific Functional Scale on interview as well as self-report basis and the data was managed by SPSS version 23.
Results

75.6% of female with chronic knee pain was found with the average pain level found to be 5 in N-NPRS scale.Among them 80.8% of chronic knee pain was due to knee osteoarthritis. Sitting function was found to be affected in 82.1% of population with mean Nepali version of Patient specific functional Scale score of 2. Similarly 80.8% reported going downstairs to be difficult due to knee pain with N-PSFS score of 2.
Conclusion

Pain and functional disability are the principle findings in patient with chronic knee pain for which they seek medical treatment .So the treatments should target on functional task with effective strategy. addressing disability. Focus on function is important for the development of optimal rehabilitation programs in patients with chronic knee pain.
High levels of low back disability, but not low back pain intensity, are associated with reduced physical activity: a community-based, cross-sectional study
Authors: Alyousef Bothaina , Kazemi Zeinab , Cicuttini Flavia M. , Heritier Stephane , Wang Yuanyuan , Urquhart Donna M. 
Published: September 2020

Abstract
Background

Although clinical guidelines recommend physical activity for low back pain (LBP), our understanding of the physical activity undertaken by people with LBP is limited. We examined the amounts, intensities and types of physical activity performed by community-based women with different levels of pain and disability.
Methods

542 women were invited to participate. Physical activity was measured using the International Physical Activity Questionnaire, and LBP and disability were assessed using the Chronic Pain Grade Questionnaire. Participants were categorised into no/low or high pain intensity and disability groups.
Results

Women with high levels of back disability performed half the total physical activity of those with no/low levels of back disability (MET(hours/week): median(95%CI) = 27.3(13.2–41.4) vs. 53.9(44.9–62.8),p = 0.002), including less moderate (17.5(10.4–24.7) vs. 32.1(26.8–37.4),p < 0.001) and domestic and gardening activity (14.4(7.2–21.7) vs. 23.5(19.0–28.0), p = 0.02). Moreover, fewer women with high disability participated in vigorous (3(8.11%) vs. 134(32.0%),OR(95%CI) = 0.17(0.04–0.75),p = 0.02) and leisure activities (17(45.9%) vs. 294(70.2%),OR(95% CI) = 0.44(0.21–0.94),p = 0.03) compared to those with no/low disability. There were no differences in physical activity between women with no/low and high pain intensity (all p > 0.05).
Conclusion

High levels of back disability, but not back pain intensity, were associated with reduced physical activity, including less total activity, moderate and vigorous intense activity, and discretionary activity. Further work is needed to determine whether targeting these types and intensities of physical activity will help in improving physical activity levels and reducing back disability.
Incidence, risk factors, and causes of maternal near-miss admitted to the intensive care unit in Yangzhou, China: A 5-year retrospective study
Authors: Chen Ying , Shi Jiaoyang , Zhu Yuting , Kong Xiang , Lu Yang , Chu Yanru , Mishu Miskatul Mustafa 
Published: September 2020

Abstract
BackgroundThe maternal near-miss (MNM) criterion formulated by the WHO can dynamically evaluate the obstetric quality and maternal health in medical institutions. The study aims to explore the incidence, risk factors, and causes of MNM cases admitted to the intensive care unit (ICU) within 5 years.MethodsThis study is a retrospective study. The data of MNM admitted to the ICU comes from the medical records of Subei People's Hospital in Yangzhou from 2015 to 2019. The study subjects meet at least one World Health Organization (WHO) criterion of MNM. The MNM who had not been admitted to the ICU in the same year served as the control group. We use descriptive analysis, Chi-Square test and Fisher’s exact test for data analysis.Results151 women met the WHO criteria of MNM and there was one maternal death in 2016. The average maternal near-miss rate（MNMR）for ICU admission was 3.5 per 1,000LBs, and the average MNM morbidity was 0.36%. The average maternal mortality ratio (MMR) was 5 per 100,000LBs. The 5-year research period witnessed moderate growth in MNM admitted to the ICU. The region, referral, gravidity, prenatal examination, and mode of delivery were significantly related to the MNM admitted to the ICU(p<0.05). Concerning neonatal characteristics, there is a significant difference in preterm birth rate and low Apgar scores at 1 min and 5 min(p<0.05). The direct obstetric causes were the primary cause of MNM, regardless of the ICU admission. The leading direct obstetric causes of MNM admitted to the ICU were obstetric hemorrhage diseases(38.8%)，following hypertension diseases(18.8%), while the leading indirect obstetric cause of MNM admitted to the ICU was heart-related diseases (7.2%). MNM for the ICU admission were mostly postpartum (96.9%), who underwent multiple interventions.ConclusionsICU is one of the most important endpoints of MNM management. In the context of "universal two-child", medical institutions should strengthen multidisciplinary joint treatment. In the future, it needs to be expanded to multi-center research to determine the criteria for MNM admitted to the ICU.
Identification and Validation of a Five-gene Signature Associated with Overall Survival in Breast Cancer Patients
Authors: Wang Xiaolong , Li Chen , Chen Tong , Zhang Hanwen , Liu Ying , Han Dianwen , Li Yaming , Li Zheng , Luo Dan , Zhang Ning , Chen Bing , Wang Lijuan , Zhao Wenjing , Yang Qifeng 
Published: September 2020

Abstract
Background

Recent years, attributed to early detection and new therapies, the mortality rates of breast cancer (BC) decreased. Nevertheless, the global prevalence was still high and the underlying molecular mechanisms were remained largely unknown. The investigation of prognosis-related genes as the novel biomarkers for diagnosis and individual treatment had become an urgent demand for clinical practice.
Methods

Gene expression profiles and clinical information of breast cancer patients were downloaded from The Cancer Genome Atlas (TCGA) database and randomly divided into training (n = 514) and internal validation (n = 562) cohort by using a random number table. The differentially expressed genes (DEGs) were estimated by Gene Ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. In the training set, the gene signature was constructed by the least absolute shrinkage and selection operator (LASSO) method based on DEGs screened by R packages. The results were further tested in the internal validation cohort and the entire cohort. Moreover, functions of five genes were explored by MTT, Colony-Formation, scratch and transwell assays. Western blot analysis was used to explore the mechanisms.
Results

In the training cohort, a total of 2805 protein coding DEGs were acquired through comparing breast cancer tissues (n = 514) with normal tissues (n = 113). A risk score formula involving five novel prognostic associated biomarkers (EDN2, CLEC3B, SV2C, WT1 and MUC2) were then constructed by LASSO. The prognostic value of the risk model was further confirmed in the internal validation set and the entire set. To explore the biological functions of the selected genes, in vitro assays were performed, indicating that these novel biomarkers could markedly influence breast cancer progression.
Conclusion

We established a predictive five-gene signature, which could be helpful for prognosis assessment and personalized management in breast cancer patients.