bluuebunny/crossref_metadata_2025_split huggingface dataset:
Feasibility and first reports of the MATCH-R repeated biopsy trial at Gustave Roussy
Authors: Recondo Gonzalo , Mahjoubi Linda , Maillard Aline , Loriot Yohann , Bigot Ludovic , Facchinetti Francesco , Bahleda Rastislav , Gazzah Anas , Hollebecque Antoine , Mezquita Laura , Planchard David , Naltet Charles , Lavaud Pernelle , Lacroix Ludovic , Richon Catherine , Lovergne Aurelie Abou , De Baere Thierry , Tselikas Lambros , Deas Olivier , Nicotra Claudio , Ngo-Camus Maud , Frias Rosa L. , Solary Eric , Angevin Eric , Eggermont Alexander , Olaussen Ken A. , Vassal Gilles , Michiels Stefan , Andre Fabrice , Scoazec Jean-Yves , Massard Christophe , Soria Jean-Charles , Besse Benjamin , Friboulet Luc 
Published: September 2020

AbstractUnravelling the biological processes driving tumour resistance is necessary to support the development of innovative treatment strategies. We report the design and feasibility of the MATCH-R prospective trial led by Gustave Roussy with the primary objective of characterizing the molecular mechanisms of resistance to cancer treatments. The primary clinical endpoints consist of analyzing the type and frequency of molecular alterations in resistant tumours and compare these to samples prior to treatment. Patients experiencing disease progression after an initial partial response or stable disease for at least 24 weeks underwent a tumour biopsy guided by CT or ultrasound. Molecular profiling of tumours was performed using whole exome sequencing, RNA sequencing and panel sequencing. At data cut-off for feasibility analysis, out of 333 inclusions, tumour biopsies were obtained in 303 cases (91%). From these biopsies, 278 (83%) had sufficient quality for analysis by high-throughput next generation sequencing (NGS). All 278 samples underwent targeted NGS, 215 (70.9%) RNA sequencing and 222 (73.2%) whole exome sequencing. In total, 163 tumours were implanted in NOD scid gamma (NSG) or nude mice and 54 patient-derived xenograft (PDX) models were established, with a success rate of 33%. Adverse events secondary to invasive tumour sampling occurred in 24 patients (7.6%). Study recruitment is still ongoing. Systematic molecular profiling of tumours and the development of patient-derived models of acquired resistance to targeted agents and immunotherapy is feasible and can drive the selection of the next therapeutic strategy.
Floral transcriptomes reveal gene networks in pineapple floral growth and fruit development
Authors: Wang Lulu , Li Yi , Jin Xingyue , Liu Liping , Dai Xiaozhuan , Liu Yanhui , Zhao Lihua , Zheng Ping , Wang Xiaomei , Liu Yeqiang , Lin Deshu , Qin Yuan 
Published: September 2020

AbstractProper flower development is essential for sexual reproductive success and the setting of fruits and seeds. The availability of a high quality genome sequence for pineapple makes it an excellent model for studying fruit and floral organ development. In this study, we sequenced 27 different pineapple floral samples and integrated nine published RNA-seq datasets to generate tissue- and stage-specific transcriptomic profiles. Pairwise comparisons and weighted gene co-expression network analysis successfully identified ovule-, stamen-, petal- and fruit-specific modules as well as hub genes involved in ovule, fruit and petal development. In situ hybridization confirmed the enriched expression of six genes in developing ovules and stamens. Mutant characterization and complementation analysis revealed the important role of the subtilase gene AcSBT1.8 in petal development. This work provides an important genomic resource for functional analysis of pineapple floral organ growth and fruit development and sheds light on molecular networks underlying pineapple reproductive organ growth.
Predicting Response to Vocabulary Intervention Using Dynamic Assessment
Authors: Gellert Anna S. , Arnbak Elisabeth 
Published: September 2020

Purpose
The purpose of this study was to examine how well students' response to a morphological vocabulary intervention can be predicted before the start of the intervention from traditional static assessments and to determine whether a dynamic assessment with graduated prompts improves the prediction.

Method
A planned secondary analysis of a randomized trial of a morphological vocabulary intervention for fifth-grade students with limited vocabulary was conducted. Response to this intervention was examined for 111 participants based on their development in definitions of morphologically transparent words from pretest to posttest. Traditional static measures of vocabulary, knowledge of morphology, and morphological analysis as well as a dynamic assessment of morphological analysis were evaluated as predictors of students' response to intervention.

Results
The static pretest measures predicted more than half of the overall variance in students' response to intervention and provided a good classification of students with subsequent poor or good response to intervention. The single best static predictor was the static assessment of morphological analysis. Furthermore, the dynamic assessment added significantly to the prediction of the overall variance in students' response to intervention and to the correct early classification of students as poor or good responders.

Conclusions
The results suggest that an acceptable level of prediction of students' response to morphological vocabulary intervention can be obtained by means of a couple of static morphological measures. This study also provides evidence for the added predictive value of a dynamic assessment of morphological analysis.
Multi‐physics and multi‐objective optimisation design of interior permanent magnet synchronous motor for electric vehicles
Authors: Song Tengfei , Liu Huijuan , Zhang Qian , Zhang Zhenyang 
Published: September 2020

The electrical machine design method plays a decisive role in electric vehicles (EVs). However, only a few designs consider the coupling of multiple fields simultaneously. This study proposes an improved multi‐physics and multi‐objective optimisation design approach for designing a 75 kW interior permanent‐magnet synchronous machine dedicated to EVs. Five optimal objectives including power density, temperature rise, price, torque ripple, and cogging torque are selected, and the first three objectives are optimised based on the sensitivity analysis of design parameters, where an improved thermal resistance network combining with finite‐element analysis model is developed. Meanwhile, the torque ripple and cogging torque are involved in subsequent design optimisation. The temperature rise and structural strength are recalculated by using commercial finite‐element model software, respectively, for validating the accuracy of optimisation design. Finally, a prototype motor is manufactured; both simulation and experimental results verify the feasibility and validity of the proposed optimisation design method.
Tensile strength of dust-ice mixtures and their relevance as cometary analog material
Authors: Haack David , Otto Katharina , Gundlach Bastian , Kreuzig Christopher , Bischoff Dorothea , Kührt Ekkehard , Blum Jürgen 
Published: September 2020

Aims. The tensile strength of granular matter is of great importance to our understanding of the evolution of comets and to our attempts to reproduce processes on cometary surfaces in laboratory experiments. In this work, we investigate the tensile strength of three different materials and their mixtures, which can be used as cometary analog materials in the laboratory.
Methods. We used two types of siliceous dusts and granular water ice whose polydisperse particles were either angular or spherical. Our samples were cooled to below 150 K to better simulate the conditions of a cometary surface and to avoid thermal alteration of the material. We used the Brazilian disk test method to exert stress on the cooled samples and determine the tensile strength at the moment the samples broke.
Results. We find that the tensile strength of two component mixtures is strongly dominated by the component with the higher tensile strength. The materials made of mostly angular dust particles have a lower filling fraction, but a higher tensile strength compared to materials made of spherical particles. Furthermore, the tensile strength of the cooled components is substantially lower than the tensile strength of the same components at room temperature. This implies that the surface energy of the investigated materials at low temperatures is significantly lower than previously assumed.
Physical Ability and Quality of Life in Rheumatoid Arthritis and Systemic Lupus Erythematosus: A Brief Comparison
Authors: Mahran Safaa , Fathi Nihal , Seddek Maha , Goma Samar H. 
Published: September 2020

Abstract
Background Patients with rheumatic disease are assumed to have low muscle performance, but few studies have been performed to prove this.
Objective To investigate and compare muscle performance in rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE) patients and detect its correlation with disease activity, physical function level and quality of life.
Patients and Methods Fifty RA patients, 50 SLE patients and 50 healthy controls were recruited for this observational, cross-sectional study. Muscle performance tests for the upper and lower limbs and the fatigue severity score were recorded. Assessments of the physical activity level using the frequency intensity time index and quality of life using the SF36 questionnaire were performed. The study was conducted over 4 months from January to April 2019.
Results SLE patients showed better results of muscle performance than RA patients; however, both had lower results as compared to control. Disease activity was correlated to muscle performance tests in both diseases, except for the 30-second chair stand test in SLE (p=0.247). All domains of SF36 had a significant correlation with the performance tests in SLE (p≤0.05); however, only domains of physical function correlated with the tests in the RA group.
Conclusion RA patients tend to have a lower muscle performance and physical activity level as compared to SLE patients and control.
Spontaneous Intraparenchymal Tension Pneumocephalus
Authors: Seo Hyukjoon , Kim Sang Hoon , Ryu Jiwook , Lee Sung Ho 
Published: September 2020

AbstractTension pneumocephalus is a treatable emergency that is usually caused by trauma or surgery. We present a rare case of spontaneous tension pneumocephalus. A 64-year-old woman presented with a severe aggravating headache. Computed tomography revealed a large air collection in the brain parenchyma of the right frontal lobe, both lateral ventricles, and the subarachnoid space. Emergent craniotomy was performed because her headache got progressively worse. We found that an abnormal bony protrusion connected the frontal sinus mucosa and the intraparenchymal pneumocephalus. After removal of the bony mass and repair of the defect, the patient immediately recovered and there was no recurrence.
Endovascular Treatment of Malignant Portomesenteric Venous Obstruction
Authors: Rostambeigi Nassir , Darcy Michael D. 
Published: September 2020

AbstractPortal vein thrombosis (PVT) is a rare condition, and malignancies account for up to a quarter of cases. Malignant PVT is an advanced stage in the neoplastic process, which affects survival. In addition, portal venous obstruction can result in portal hypertension and associated complications. There are a few reports on the surgical and nonsurgical management of this condition. We herein aim to present an overview of these management options and discuss the factors affecting the outcomes after each. We will also discuss gaps in knowledge and the possible areas for future research.
Electronic Health Record Use among Ophthalmology Residents while on Call
Authors: Long Christopher P. , Tai-Seale Ming , El-Kareh Robert , Lee Jeffrey E. , Baxter Sally L. 
Published: September 2020

Abstract
Background As electronic health record (EHR) use becomes more widespread, detailed records of how users interact with the EHR, known as EHR audit logs, are being used to characterize the clinical workflows of physicians including residents. After-hours EHR use is of particular interest given its known association with physician burnout. Several studies have analyzed EHR audit logs for residents in other fields, such as internal medicine, but none thus far in ophthalmology. Here, we focused specifically on EHR use during on-call shifts outside of normal clinic hours.
Methods In this retrospective study, we analyzed raw EHR audit log data from on-call shifts for 12 ophthalmology residents at a single institution over the course of a calendar year. Data were analyzed to characterize total time spent using the EHR, clinical volume, diagnoses of patients seen on call, and EHR tasks.
Results Across all call shifts, the median and interquartile range (IQR) of the time spent logged into the EHR per shift were 88 and 131 minutes, respectively. The median (IQR) unique patient charts accessed per shift was 7 (9) patients. When standardized to per-hour measures, weekday evening shifts were the busiest call shifts with regard to both EHR use time and clinical volume. Total EHR use time and clinical volume were greatest in the summer months (July to September). Chart review comprised a majority (63.4%) of ophthalmology residents' on-call EHR activities.
Conclusion In summary, EHR audit logs demonstrate substantial call burden for ophthalmology residents outside of regular clinic hours. These data and future studies can be used to further characterize the clinical exposure and call burden of ophthalmology residents and could potentially have broader implications in the fields of physician burnout and education policy.
The Effect of a 20-Hour Baby-Friendly Hospital Initiative Training Program on Nurses’ Breastfeeding Knowledge, Attitudes and Confidence, in a Tertiary Hospital in Singapore
Authors: Fok Doris , Chang Hsin Fang , Meng Ling Yan , Ng Yvonne Peng Mei 
Published: September 2020

Objective The Baby-Friendly Hospital Initiative (BFHI) enables maternity units to be centers of breastfeeding support to increase breastfeeding rates. This study evaluates the impact of the 20-hour BFHI training course on nurses' breastfeeding knowledge, attitude, and confidence in breastfeeding practice in a tertiary hospital in Singapore.
Study Design Seventeen sessions of the 20-hour BFHI training course were conducted by lactation consultants from 2010 to 2013 at the National University Hospital, Singapore. An anonymous self-administered survey on knowledge, attitude, and confidence in breastfeeding practices were distributed to nurses before (2009) and after (2014) the training courses to assess effectiveness of training.
Results One-hundred forty nurses and one hundred forty-eight nurses participated in the surveys in 2009 and 2014, respectively. Majority were registered nurses who worked in the postnatal wards and the neonatal intensive care unit. After training, there were significant improvements for five of eight items in infant feeding knowledge, including greater awareness of the International Code of Marketing of Breastmilk Substitutes and medical contraindication for breastfeeding. Participants reported more confidence in assisting mothers on breastfeeding, 77.1 to 88.5% (p = 0.019); advising hand expressing breast milk, 75.7 to 86.5% (p = 0.012); and advising attachment to the breast, 75.7 to 89.2% (p = 0.004) in 2014 compared with 2009. However, despite having high levels of confidence, only about half the nurses reported being able to assist mothers in breastfeeding, mainly due to time constraints.
Conclusion Implementation of the 20-hour BFHI training program positively influenced nurses' breastfeeding knowledge, attitude, and confidence in breastfeeding practices. Hospital procedures and manpower requirements should be re-examined to overcome nursing constraints in providing breastfeeding help to postpartum mothers.
Key Points
Modulation of acyl-carnitines, the broad mechanism behindWolbachia-mediated inhibition of medically important flaviviruses inAedes aegypti
Authors: Manokaran Gayathri , Flores Heather A. , Dickson Conor T. , Narayana Vinod K. , Kanojia Komal , Dayalan Saravanan , Tull Dedreia , McConville Malcolm J. , Mackenzie Jason M. , Simmons Cameron P. 
Published: September 2020

SignificanceWolbachia(wMel strain)-infectedAedes aegyptimosquitoes are refractory to disseminated arboviral infections. Yet previous studies into the mechanism behindWolbachia-mediated virus blocking have not considered the involvement of lipids, apart from cholesterol, during superinfection. We used liquid chromatography mass spectrometry to study the lipidome in mosquito cells infected with virus,wMel, or superinfected with both virus andwMel. Interestingly, a class of lipids, acyl-carnitines increased during virus infection but remained low withwMel. These findings uncover a previously unknown role for acyl-carnitines in the interaction among virus,wMel, and cells, suggesting a mechanism underlyingWolbachia-mediated pathogen blocking. Importantly, this study supportsWolbachiaintrogression intoA. Aegyptipopulations as a biocontrol method to reduce arboviral (e.g., DENV) transmission.
Tuning the Baird aromatic triplet-state energy of cyclooctatetraene to maximize the self-healing mechanism in organic fluorophores
Authors: Pati Avik K. , El Bakouri Ouissam , Jockusch Steffen , Zhou Zhou , Altman Roger B. , Fitzgerald Gabriel A. , Asher Wesley B. , Terry Daniel S. , Borgia Alessandro , Holsey Michael D. , Batchelder Jake E. , Abeywickrama Chathura , Huddle Brandt , Rufa Dominic , Javitch Jonathan A. , Ottosson Henrik , Blanchard Scott C. 
Published: September 2020

Significance
Fluorescence imaging, a central pillar of medical and fundamental research, is hampered by stochastic blinking, limited photon budgets, and phototoxicities of organic fluorophores. Here, we use quantum chemical calculations and targeted synthetic strategies to tune the Baird aromatic triplet-state energy of cyclooctatetraene to enhance its capacity to mediate intramolecular triplet−triplet energy transfer with distinct organic fluorophores spanning the visible spectrum. By tuning the efficiency of the self-healing mechanism, we generate cyanine-class fluorophore derivatives with improved intrinsic brightness and photostability, and reduced reactive oxygen species generation. We leverage these advances to extend the time scale of camera-based, single-molecule imaging techniques, including Förster (fluorescence) resonance energy transfer methods, to the kilohertz regime in fully oxygenated physiological buffers.
Mechanical design of the highly porous cuttlebone: A bioceramic hard buoyancy tank for cuttlefish
Authors: Yang Ting , Jia Zian , Chen Hongshun , Deng Zhifei , Liu Wenkun , Chen Liuni , Li Ling 
Published: September 2020

Significance
Cellular solids, or foams, are an important class of structural materials for packaging, transportation, and infrastructure due to their mechanical efficiency. Current foams are primarily made of metallic or polymeric materials, while ceramics could potentially serve this goal better, as they have higher specific stiffness and strength, and are chemically more stable. What limits the application of ceramic cellular solids as structural components are their brittleness and flaw sensitivity. Overcoming the fragile nature of ceramic foams, making them lighter while reaching higher stiffness, strength, and energy absorption is challenging but critical toward many applications. In this study, we elucidate how cuttlebone overcomes its intrinsic brittleness through the “wall–septa” microstructure, which could inspire the development of lightweight ceramic cellular solids.
Propagation.
Authors: Mitra Sisir 
Published: September 2020

Abstract

This chapter discusses propagation of papaya by seed, cutting, grafting, budding, and in vitro method (embryogenesis). Papaya is mainly propagated by seeds. The best seed for germination of seedlings is obtained from ripe or overripe fruits. Environmental factors, including temperature and light influence seed germination. Clonal propagation by cutting, grafting, budding and micropropagation have been standardized, but are still not used commercially in most of the countries where papaya is grown. The advantages of using clonally propagated plants are that the sex of the plant is known, they are more vigorous and earlier in bearing than seedling plants. There is good potential for adoption of clonal propagation when production becomes efficient enough to compete in price with the current practice of planting two to three seedlings in a single hole and thinning after 3-4 months.
Physiological disorders.
Authors: Tajidin Nor E. , Munirah Mohamad , Shukor Nur I. A. , Ahmad Siti H. 
Published: September 2020

Abstract

This chapter focuses on the various physiological disorders of pawpaws, which can be recognized as abnormal growth conditions, either outside or inside the fruits, thus affecting the appearance and flavour qualities of the fruits and eventually their marketability. Information on the various factors causing these physiological disorders are also presented.
Nutrigenomics and fish
Authors: Mohanty Bimal Prasanna , Ganguly Satabdi , Mahanty Arabinda , Mitra Tandrima , Mohanty Sasmita 
Published: September 2020

Abstract
The interaction of nutrients, environment, and genome determines the general physiological functioning and health of an organism. The branch of science which aims to understand these interactions is known as “Nutrigenomics.” Aquaculture and fisheries are the fast-growing food production systems and have an important role to play in achieving food and nutritional security. However, like other food production sectors, it also faces numerous challenges. Understanding the nutritional requirements and need-based dietary manipulation are perhaps the most effective and sustainable way of dealing with these challenges. In this review, we discuss the concept of nutrigenomics as a subset of multi-omics technology and highlight some studies encompassing nutrigenomics studies, with a specific focus on its application in fish nutrition. How these emerging techniques are applied in understanding various aspects of fish physiology as influenced by nutrition including nutritional supplements and various environmental factors like habitat, stress (temperature), and environmental contaminants have also been discussed.
Integration of buried piezoresistive sensors and PZT thin film for dynamic and static position sensing of MEMS actuator
Authors: Vergara Andrea , Tsukamoto Takashiro , Fang Weileun , Tanaka Shuji 
Published: September 2020

Abstract
We developed a lead zirconate titanate (PZT) thin film actuator integrated with buried piezoresistors for the dynamic and static deformation sensing of a PZT MEMS actuator. We demonstrated the fabrication of sol-gel deposited PZT thin film devices combined with buried piezoresistors and proved, for the first time, the process compatibility of these materials. Dopant concentration measured by secondary ion mass spectrometry (SIMS) analysis confirms that the piezoresistor was successfully buried into the device. Motion detection of the fabricated MEMS cantilever actuated by the PZT thin film was successful and consistent with optical measurement as well as design values. From these results, we can conclude that our PZT actuator and piezoresistive sensors can be monolithically integrated. The fabrication process developed here can be used for high-stability piezoelectric MEMS actuators with feed-back control of position.