bluuebunny/crossref_metadata_2025_split huggingface dataset:
Presenilin 1 and APP Gene Mutations in Early-Onset AD Families from a Southeast Region of China
Authors: Zhou Jiajia , Chen Yi , Meng Fanxia , Zhang Kan , Liu Xiaoyan , Peng Guoping 
Published: June 2020

Background:
Early-Onset Familial Alzheimer’s Disease (EOFAD) has been reported to be
associated with Presenilin 1 (PSEN1), Presenilin 2 (PSEN2), and Amyloid Precursor Protein (APP)
genes. The spectrum of mutations in Chinese patients with EOFAD was rarely investigated.

Objective:
To investigate the spectrum of mutations in patients with EOFAD in Chinese population.

Methods:
We performed whole-exome sequencing and described relevant clinical features in a total of
67 subjects from 3 families with EOFAD.

Results:
A splice mutation (p.S290C) in PSEN1 and a missense mutation (p.V717I) in APP were identified.

Conclusion:
The variant p. S290C (c.869-2>G) in PSEN1 in Chinese EOAD family revealed different
clinical phenotypes when compared with that of Europeans.
Evaluation and Prediction of Early Alzheimer’s Disease Using a Machine Learning-based Optimized Combination-Feature Set on Gray Matter Volume and Quantitative Susceptibility Mapping
Authors: Kim Hyug-Gi , Park Soonchan , Rhee Hak Y. , Lee Kyung M. , Ryu Chang-Woo , Lee Soo Y. , Kim Eui J. , Wang Yi , Jahng Geon-Ho 
Published: June 2020

Background:
Because Alzheimer’s Disease (AD) has very complicated pattern changes, it is
difficult to evaluate it with a specific factor. Recently, novel machine learning methods have been applied
to solve limitations.

Objective:
The objective of this study was to investigate the approach of classification and prediction
methods using the Machine Learning (ML)-based Optimized Combination-Feature (OCF) set on Gray
Matter Volume (GMV) and Quantitative Susceptibility Mapping (QSM) in the subjects of Cognitive
Normal (CN) elderly, Amnestic Mild Cognitive Impairment (aMCI), and mild and moderate AD.

Materials and Methods:
57 subjects were included: 19 CN, 19 aMCI, and 19 AD with GMV and QSM.
Regions-of-Interest (ROIs) were defined at the well-known regions for rich iron contents and amyloid
accumulation areas in the AD brain. To differentiate the three subject groups, the Support Vector
Machine (SVM) with the three different kernels and with the OCF set was conducted with GMV and
QSM values. To predict the aMCI stage, regression-based ML models were performed with the OCF set.
The result of prediction was compared with the accuracy of clinical data.

Results:
In the group classification between CN and aMCI, the highest accuracy was shown using the
combination of GMVs (hippocampus and entorhinal cortex) and QSMs (hippocampus and pulvinar) data
using the 2nd SVM classifier (AUC = 0.94). In the group classification between aMCI and AD, the highest
accuracy was shown using the combination of GMVs (amygdala, entorhinal cortex, and posterior
cingulate cortex) and QSMs (hippocampus and pulvinar) data using the 2nd SVM classifier (AUC =
0.93). In the group classification between CN and AD, the highest accuracy was shown using the combination
of GMVs (amygdala, entorhinal cortex, and posterior cingulate cortex) and QSMs (hippocampus
and pulvinar) data using the 2nd SVM classifier (AUC = 0.99). To predict aMCI from CN, the exponential
Gaussian process regression model with the OCF set using GMV and QSM data was shown the most
similar result (RMSE = 0.371) to clinical data (RMSE = 0.319).

Conclusion:
The proposed OCF based ML approach with GMV and QSM was shown the effective performance
of the subject group classification and prediction for aMCI stage. Therefore, it can be used as
personalized analysis or diagnostic aid program for diagnosis.
Design, Synthesis, Docking and Computational Pharmacokinetic Profiling of New Pyrazolinyl Thiazolinone Biheterocycles as Potent Antimicrobial Agents
Authors: Salian Vinutha Vittala , Narayana Badiadka , Sarojini Balladka Kunhanna , Kodandoor Sharath Chandra , Lobo Anupam Glorious 
Published: June 2020

Background:
Development of potential antimicrobial agents is the main aim in the drug
discovery process to overcome the problem of drug resistance. Pyrazolines and thiazolinones are extensively
used as building blocks for the synthesis of diverse and medicinally important compounds.

Methods:
In this present work, a new series of functionalized pyrazolinyl-thiazolinone biheterocycles
is designed and synthesized from N-pyrazolinecarbothioamide. Antimicrobial screening is carried
out in order to discover their potential towards six bacterial and four fungal strains. The zone of
inhibition (ZI in mm) was determined by the disc diffusion method and minimum inhibitory concentration
(MIC in μg/mL) by macro dilution method. The druggability of these new entities is done
through in silico pharmacokinetic profiling using Maestro 2017-1 interface of Schrӧdinger software.

Results and Disscusion:
Compounds 4c and 4e with chloro and iodo substituents on Nphenylacetamide
ring displayed good inhibitory antibacterial activity against the tested bacterial
strains with minimum MIC values when compared to the reference drug tetracycline. Compound 3
with an acetic acid derivative showed high antifungal activity among all the tested derivatives.
Compound 3 not only showed antifungal activity but also qualified druggability test with no violation
of Lipinski rule of five.

Conclusion:
The capability of the synthesized pyrazolinyl-thiazolinone derivatives was performed
to efficiently inhibit the growth of microorganisms against selected bacterial and fungal strains. Further,
these compounds are found to be effectively bound to the active sites of attractive target Escherichia
coli FabH.
Relative Validity and Repeatability of a Zinc-FFQ for Estimating Intakes of Iron, Copper and Selenium in Saudi Adults
Authors: Alsufiani Hadeil M. , Albar Salwa A. , Al-Shehri Aishah D. , Al-Mufti Zain A. , Aldahri Rahaf S. , Omar Ulfat M. 
Published: June 2020

Background:In Saudi Arabia, few studies have addressed iron, selenium and copper intake and status. Most studies on iron have focused on iron deficiency anemia rather than the intake of iron.Objective:To assess the relative validity and repeatability of a Zinc-food Frequency Questionnaire (FFQ) for estimating the intakes of iron, copper and selenium in Saudi adults.Methods:Ninety-seven Saudi adults participated in this study. All participants completed the FFQ1 and a three-days (3d) Food Record (FR) to assess validity. After one month, the FFQ was administered again (FFQ2) to assess the repeatability.Results:Mean intakes of iron, copper and selenium estimated by FFQ1 were significantly higher than those from the 3d FR. Estimated intakes of iron and selenium by both methods were significantly correlated. The Bland-Altman analysis showed that the difference in iron intakes, as measured by the two methods, was similar across the range of intakes while the difference in copper and selenium intakes increased with an increase in the mean intake. No detectable differences were observed between FFQ1 and FFQ2 in estimating iron, copper and selenium intakes. For all nutrients, the intakes from both FFQs were highly correlated.Conclusion:The Zinc-FFQ showed reasonable relative validity and repeatability for estimating other micronutrients, such as iron, copper and selenium.
Determination of three Unsaturated Fatty Acids in Pressure Ulcer Rats Using A UPLC-MS/MS Method
Authors: Cai Fuman , Dong Yuwei , Lou Shaosheng , Ma Zeping , Wu Ting , Yang Liuqing , Wen Congcong , Wang Chenxiang 
Published: June 2020

Background:The serum levels of Docosahexaenoic Acid (DHA), Eicosapentaenoic Acid
(EPA) and Arachidonic Acid (AA) under the state of Pressure Ulcers (PUs) are still unclear.

Introduction:In order to investigate serum levels of DHA, EPA, and AA in PUs rats, an ultraperformance
liquid chromatography-tandem mass spectrometry (UPLC-MS/ MS) method was developed
and validated.

Methods:Chromatographic separation of DHA, EPA, AA was carried out on a BEH C18 column and
gradient elute consisted of 5 mM ammonium acetate-0.1% formic acid and acetonitrile. Subsequently,
fifty rats were divided into five groups (n=10), four PU groups (A-D) underwent various pressure and
release time protocols, with group E as the control. The concentrations of DHA, EPA, AA from five
groups were determined by using a validated method.

Results:The results showed there was good linearity for DHA (327.3/283.4), EPA (301.2/257.0), and
AA (303.1/258.9) within 0.05-6.4 μg/mL. In control group, the levels of DHA, AA and EPA were
1.16±0.68, 0.59±0.19 and 0.78±0.21 μg/mL. At the end of modeling, concentrations of DHA, EPA and
AA were increased after long and persistent pressure (>8 h). Especially, the level of DHA was significantly
higher (P<0.01) than that of control group.

Conclusion: A stable, rA stable, reliable and accurate UPLC-MS/MS for determination of DHA, EPA, AA in
blood was developed. Serum concentrations of DHA, EPA and AA were altered differently after long
and persistent pressure (>8 h), and DHA is a remarkable one.eliable and accurate UPLC-MS/MS for determination of DHA, EPA, AA in blood was developed.
Serum concentrations of DHA, EPA and AA were altered differently after long and persistent pressure (>8 h), and DHA is
a remarkable one.
Metabolic Changes in Mouse Plasma after Acute Diquat Poisoning by UPLC-MS/MS
Authors: Chen Lianguo , Zhong Zuoquan , Liu Jiawen , Wen Congcong , Jin Yongxi , Wang Xianqin 
Published: June 2020

Introduction:
Diquat is a fast-acting contact herbicide and plant dehydrating agent. The oral lethal dose 50
(LD50) of diquat in mice is about 125 mg/kg. The purpose of this study is to research the metabolomics in mouse plasma
after acute diquat poisoning.

Method:
These mice were divided into two groups (the control group and acute diquat poisoning group). The control
group was given normal saline by gavage. The acute diquat poisoning group was given 50 mg/kg diquat. UPLC-MS/MS
was used to determinate the small molecule organic acid in mouse plasma.

Results:
Compare to the control group, the L-lysine, Adenine, L-Alanine, L-Valine, Lactic acid, Inosine, Adenosine, LTryptophan, L-Tyrosine, L-Arginine, L-Phenylalanine, L-Methionine, Citric acid, Fructose, L-Glutamine, Malic acid, LAspartic acid and Pyruvic acid increased in the acute diquat poisoning group (p<0.05); while the L-Histidine decreased
(p<0.05).

Conclusion:
The results of metabolites increased or decreased, indicating that acute diquat poisoning induced amino acid
metabolism and energy metabolism perturbations in mice.
Phloretin Ameliorates Acetic Acid Induced Colitis Through Modulation of Immune and Inflammatory Reactions in Rats
Authors: V.S. Arya , S.K. Kanthlal 
Published: June 2020

Background:
Adverse effects associated with current therapy for Ulcerative colitis (UC)
over prolonged treatment periods and the high relapse rate limit their use. Incorporating fruits as regular
diet has beneficial role in the management of UC. Phloretin, a dihydrochalcone of apple is reported
for its anti-oxidant and anti-inflammatory effects. Our study aims to evaluate the effectiveness of phloretin
on experimentally induced ulcerative colitis in rats.

Methods:
In vitro study was performed using Raw 264.7 cells stimulated with LPS (1μg/mL) and in
in-vivo study, colitis was induced by intra rectal administration of 4% Acetic acid. Phloretin (50
mg/kg) was given orally for 3 days to Wistar rats after induction for the post-treatment group and 1 day
before induction to the pre-treatment group. Macroscopical, biochemical and histopathological evaluations
were performed to assess the effectiveness.

Results:
A concentration dependent inhibition of MPO and iNOS activity was obtained in LPS stimulated
neutrophil cells. Phloretin exerted ameliorative effect in both pre and post-treatment groups by
restoring plasma ALP and LDH level and reduce inflammatory markers like myeloperoxidase, nitric
oxide and eosinophil peroxidase level as well as downregulates colon ICAM-1 gene in acetic acid induced
ulcerative colitis in rats. Antioxidative potency was confirmed by restoring tissue GSH level.
Phloretin prevents mucosal damage and it was confirmed by histopathological analysis.

Conclusion:
Collectively, our findings provide evidence that phloretin might be useful as a natural
therapeutic agent in the management of UC as well as may pose a promising outcome for future clinical
usage.
Serum Thyroid Peroxidase Antibody Level and Incident Hypertension in Iranian Men: A Suggestion for the Role of Thyroid Autoimmunity
Authors: Tohidi Maryam , Baghbani-Oskouei Aidin , Amouzegar Atieh , Mehran Ladan , Azizi Fereidoun , Hadaegh Farzad 
Published: June 2020

Background:
Dysfunction of the thyroid gland has profound effects on the cardiovascular
system.

Objective:
We aimed to explore the relation of serum thyroid peroxidase antibody (TPO-Ab), as a
marker of thyroid autoimmunity with incident hypertension among a euthyroid population.

Methods:
A total of 3681 participants (1647 men) entered the study. Multivariate Cox proportional
hazard models were conducted to estimate the association between TPO-Ab and incident hypertension.

Results:
The mean age (standard deviation) of the participants was 37.5 (12.8) years. During a median
follow-up of 12.2 years, 511 men and 519 women developed hypertension. The multivariable hazard
ratios (HRs) and related 95% confidence intervals (CIs) of 1-unit increase in natural logarithm (ln) of
TPO-Ab for incident hypertension were 1.09 (1.00-1.19), 1.03 (0.97-1.10), and 1.05 (1.00-1.11) for
men, women, and total population, respectively. Moreover, considering the TPO-Ab status as a categorical
variable (i.e. TPO-Ab positive or TPO-Ab negative), the multivariate-adjusted HRs (95% CIs)
of TPO-Ab positivity for incident hypertension, were 1.33 (0.95-1.85), 1.12 (0.86-1.45) and 1.19 (0.97-
1.46) for men, women, and total population, respectively.

Conclusion:
Elevated serum TPO-Ab level can contribute to the development of hypertension among
euthyroid men during a long follow-up; suggesting a role for thyroid autoimmunity.
A Survey on Low Power Design Approaches in Nanoscale Regime
Authors: Sharma Vijay Kumar 
Published: June 2020

Background:
The increased demand for battery operated portable systems boost up the
field of low power VLSI design. Integrated circuits are enhancing the performance of the systems in
terms of lesser area requirement, higher functionality, and faster response at lower technology nodes.
The applied power supply and the threshold voltage of the individual device are scaled down at lower
technology node. Scaling of the threshold voltage of the devices raises the issue of leakage current.

Objective:
Leakage current should be made recessive with the continuous scaling of technology nodes.

Methods:
Various leakage current mitigation methods had been employed to reduce the leakage current
at different abstraction levels. This review paper demonstrates the survey of systematic arrangement
of device scaling, leakage power, its causes, and various methods to overcome the leakage current
at circuit level design.

Results:
3 input NAND (NAND3) gate is designed and simulated at 22 nm technology node on
HSPICE tool and analyzed for comparison of different leakage reduction techniques.

Conclusion:
INDEP approach is the most effective approach to reduce the leakage current and to improve
the reliability of the circuits followed by DTCMOS technique as compared to other available
techniques.
Intelligent Service and Metadata Management for Smart IoT Middleware
Authors: Park Jong-Hyun 
Published: June 2020

Background:
In IoT (Internet of Things) environment, a number of sensors and networks
exist and they have various and heterogeneous characteristics. Applications, which provides a variety
of services based on the sensor networks, also have different service requirements. Therefore, a middleware
that is located between sensor networks and application systems is needed for integrating
two layers.

Objective:
This paper proposes a general-purpose middleware for providing intelligent services based
on heterogeneous sensors existing in the IoT environment.

Methods:
The proposed middleware acquires and manages sensing data in real-time. The middleware
stores and manages heterogeneous sensors, node, and network metadata. In addition, the middleware
infers the situation based on ontologies and rules and provides intelligent services.

Results:
The paper has proposed integrated metadata to describe heterogeneous sensor, node and
network information. The third is the intelligent service management system to provide sensor-based
intelligent service to users. The paper has proposed the SS ontology for describing sensors and services
and proposed a method for providing intelligent services using the SS ontology and rules. Of
course, the three systems operate independently, so they can be used selectively as needed. This paper
implemented a prototype system based on the proposed method.

Conclusion:
We expect that the proposed middleware can be used in various applications for sensorbased
intelligent service processing. In the future, we plan to expand the method to provide an intelligent
service by automatically gathering semantic data.
Evolution of Clinical Outcome During Adolescents’ Psychiatric Inpatient Care: A Prospective Multiple Informant Study
Authors: Cairó Marta Ruiz , Urben Sébastien , Terren Morgane , De Rocquigny Hélène , Courossé Swen , Bisio Cecilia , Caspani Vivien , Legoux Céline , Petraglia Géraldine , Guignet Boris , Plessen Kerstin Jessica , Holzer Laurent 
Published: June 2020

Background:
Examining the effectiveness and adequacy of adolescents’ psychiatric
inpatient treatment through multiple perspectives is crucial to provide the best care.

Objectives:
The aims of the current study were to examine the consistencies and discrepancies
between patients and clinicians and to understand how each group considered
the timing of improvement of symptoms and psychosocial difficulties of adolescents
during a psychiatric inpatient stay.

Methods:
The Health of the Nation Outcome Scales for Children and Adolescents
(HoNOSCA, assessing symptoms and psychosocial difficulties) was rated on a weekly
basis by patients and clinicians during a psychiatric inpatient stay. Data were collected
from 297 patients, 58.2% females.

Results:
Both clinicians and patients reported a significant decrease of the HoNOSCA
scores from admission to discharge, revealing that inpatient treatment is perceived as
helping the adolescents to alleviate their symptoms and psychosocial difficulties. However,
the item-by-item analyses indicated that patients and clinicians reported difficulties
in different symptoms and psychosocial domains. Moreover, the week-by-week
analyses revealed discrepancies in the perception of the time-course of clinical outcome-
changes between clinicians and patients, as well as between males and females,
and between voluntarily and involuntarily admitted patients.

Conclusions:
By integrating perspectives of patients and clinicians and their respective
timelines, as well as by taking into account the mode of admission and the patient’s
gender, this study provides a deeper understanding of the evolution of clinical outcome
during adolescents’ hospitalizations, which allows to adapt their treatment and therewith,
to help patients more efficiently.
Kajian evaluasi budaya organisasi Perpustakaan Nasional Republik Indonesia
Authors: Salahudin Salahudin , Nurmandi Achmad , Santoso Joko , Kusumawardhani Budi , Rusdianto Rusdianto , Agusta Rendra , Loilatu Mohammad  Jafar 
Published: June 2020

Introduction. This study aims to determine the organizational culture in the National Library of Indonesia (NLI) based on several aspects including clan (human resource management), adhocracy (innovation organization), market (adaptation and strategic organization), and hierarchy (coordination and organizational structure).
Research Methods. This study used a quantitative approach of Competing Values ​​Framework (CVF) developed by Cameron & Quinn (2011) to obtain data scores and the average of each aspect of organizational culture.
Data Analysis. Organizational Culture Assessment Instrument (OCAI) is an organizational culture assessment instrument by using statements/questions relating to the six subsystems, namely organizational culture dominant characters, leadership, human resource management, internal solidarity, strategic management and organizational success criteria. The data was analysed by using SPSS with Pearson Correlation formula.
Results and Discussion. We found that organizational culture of the National Library is cultural hierarchy.
Conclusion and Recommendations. Organization culture of the (NLI) shows the cultural hierarchy. The cultural hierarchy is maintained as part of the working principles of the organization.
Hazardous Ice Phenomena In Rivers Of The Russian Arctic Zone Under Current Climate Conditions And The Safety Of Water Use
Authors: Agafonova Svetlana A. , Vasilenko Alexander N. 
Published: June 2020

The ice regime of the Russian Arctic rivers and its hazardous manifestations under current climate conditions are characterized. The ice phenomena in rivers in the region determine the conditions of navigation, water supply, hydropower station (HPS) operation, and the construction of temporary ice bridges and roads. Data of more than 100 hydrological gages over period from 1936 to 2016 were used to compile various cartographic materials and to analyze the spatial variations of the dates of ice phenomena, the duration of ice-free and ice cover periods, and the maximal ice thickness. Special attention is paid to the characteristics of level regime in periods with ice phenomena. Data on the frequency of floodplain inundation during spring ice run, the hazard of ice jams, and the seasonal features of the passage of maximal annual water level are generalized.The observed changes in ice regime characteristics and ice hazard are analyzed. The years of the start of statistically significant shift of the periods of ice phenomena, caused by both climate changes and anthropogenic impact, are identified. The increase in the duration of the ice-free period was found to be not greater than 3–4 days for East Siberian rivers, 5–6 days for the Middle and West Siberian rivers, and up to 10–12 days for the rivers in the European part. A decrease in the maximal ice thickness is most pronounced in the rivers of the Northern European Russia, where it is 10–15 cm. The frequency of floodplain inundation during spring ice run remains constant.