bluuebunny/crossref_metadata_2025_split huggingface dataset:
LncRNA XIST acts as a microRNA-520 sponge to regulate the cisplatin resistance in NSCLC cells by mediating BAX through ceRNA network
Authors: Liu Ting-Ting , Li Rui , Liu Xiao , Zhou Xi-Jia , Huo Chen , Li Jian-Ping , Qu Yi Qing 
Published: May 2020

Abstract
Background: In recent years, LncRNA acts as a member of competing endogenous RNA (ceRNA), playing an important role in drug resistance of lung cancer. The aim of this study was to identify potential biomarkers about cisplatin resistant lung cancer cells using a comprehensive ceRNA network.Methods: GSE6410 (GPL-201) analyzed gene expression changes about cispaltin resistance in A549 NSCLC cells. GSE43249 (GPL-14613) included noncoding RNA expression profiling derived from the cisplatin resistant A549 lung cells. GEO2R, an online analysis tool, analyzed the differentially expressed mRNAs and miRNAs (DEmRNAs and DEmiRNAs). To explore the functional enrichment implication of differentially expressed mRNAs, we used the GO and KEGG pathway analysis. Through miRDB、Targetscan、Starbase、miRWalk, we found targeted miRNAs. The Kaplan-Meier curve method was used to show clinical survival analysis of targeted RNAs (P<0.05). The Starbase database predicted potential lncRNAs mediated targeted miRNAs. Eventually, the novel ceRNA network of lncRNAs, miRNAs, mRNA was constructed by cytoscape3.7.2.Results:118 differentially expressed mRNAs were the basis of the mediated ceRNA network. DAVID and Kaplan-Meier picked out BAX, an apoptosis regulator. Venn Diagram demonstrated 8 miRNAs commomly regulating Bax. Starbase predicted lncRNA XIST mediated miRNAs. Finally, lncRNA XIST maybe a useful biomarker regulating cisplatin resistance in lung cancer cells.Conclusions: LncRNA XIST competitively bound to miRNA 520 in the regulation of cisplatin resistance by BAX , participating apoptosis in the p53 signaling pathway.
Clinical and radiological characteristics of brain abscess caused by Streptococcus species: a cross-sectional study
Authors: Su Jiachun , Hu Bin , Zhang Yixin , Li Ying 
Published: May 2020

Abstract
Background: Streptococcus species are mostly reported the predominant organisms causing brain abscess (BA), however, there is no article focusing on the characteristics of patients with BA caused by Streptococcus species to date. This study aimed to describe the clinical and radiological features of patients with BA caused by Streptococcus species. Methods: All patients who were hospitalized due to BA with intracranial pus culture result of Streptococcus species alone after neurosurgical intervention from May 2015 to August 2018 in Huashan Hospital in Shanghai were included in this study. The data of patient demographics, clinical and radiological presenting features, microbiological results, surgical and antimicrobial treatment, and outcomes were collected. Results: Thirteen patients (10 male, 3 female) of primary brain abscesses were involved. The most frequent clinical presentations included headache (76.9%), fever (38.5%) and limb weakness (38.5%).The radiological features of abscesses in these patients included singularity, big size, thick wall and obvious oedema. The average abscess diameter before surgery was 51.62±11.00mm. The mean thickness of abscesses wall was 5.23±1.78mm. The mean range of oedema and distance between abscess margin were 93.62±16.31mm, 26.38±8.03mm, respectively. The strains isolated in this study all belonged to the viridans group and showed high susceptibility to cefotaxime and levofloxacin. All patients had good outcomes at discharge. Conclusion: Patients with BAs caused by Streptococcus species had unspecific clinical signs, but specific radiological features, which might be helpful for early diagnosis.
Endoscopic surgical treatment with nasal cavity approach for chondrosarcoma of paranasal sinus and the skull base
Authors: Gao Junxiao , Zhu Zhenchao , Ye Yudong , Qiu Qianhui , Fu ming 
Published: May 2020

Abstract
Background Chondrosarcoma(ChSa) is a rare malignant tumor. But it’s necessary to discuss the clinical characteristics and treatments for ChSa of paranasal sinus and the skull base.We aimed to Observe the effect of Endoscopic surgical treatment with nasal cavity approach for chondrosarcoma of paranasal sinus and the skull base
Methods The clinical characteristics of chondrosarcoma of paranasal sinus and skull base in 10 patients (6 males and 4 females) from 2001 to 2017 were analyzed. They all underwent by endoscopic surgery. The patients’ age ranged from 18 to 47 years, with a median of 35 years. Clinical symptoms: stuffy, nose bleeding, runny, headache, diplopia, eye outreach limited, blurred vision and even blindness. Surgery methods：under nasal endoscopy, after the attachment sites of the tumors to normal tissues were confirmed, the tumors were peeled off along the clear boundary between the tumors and normal tissues, and the potential residual tumor tissues on bones were cleared by a drill. If internal carotid artery (ICA) were involved, flap would be used to protect it.
Objectives Results All patients were treated with endoscopic surgery，followed up postoperatively for 24 to 108 months, with a median of 36 months. 8 of 10 patients were no recurrence,2 were alive with tumor.
Conclusion Chondrosarcoma of paranasal sinus and skull base can be treated by nasal endoscopic surgery with good clinical results.
WITHDRAWN: Treatment for Lumbar Spinal Stenosis Secondary to Ligamentum Flavum Hypertrophy Using Percutaneous Endoscopy through Interlaminar Approach: A Retrospective Study
Authors: Liu Yi , Qi Yingjie , Diaty Diarra Mohamed , Zheng Guanglei , Shen Xiaoqiang , Lin Shangben , Chen Jiaqi , Song Yongwei , Gu Xiaomin 
Published: May 2020

Abstract

Background
This paper is to first describe percutaneous endoscopy in the treatment of lumbar spinal stenosis secondary to ligamentum flavum hypertrophy targetedly. To investigate the efficacy and safety of percutaneous endoscopy in the treatment of this kind of lumbar spinal stenosis in elderly patients.
Method:
A retrospective analysis of 40 elderly patients with lumbar spinal stenosis secondary to ligamentum flavum hypertrophy admitted between January 2016 and January 2018 was performed. According to different surgical methods, they were divided into two groups: the control group and the endoscopy group (interlaminar approach), 20 people per group. There were 9 males and 11 females in the control group; the age of patients was 65.65 ± 4.44years, and the average disease duration was 4.55 ± 1.85 years. Besides, There were 10 males and 10 females in the endoscopy group; the age of patients was 67.30 ± 4.23years, and the average disease duration was 4.95 ± 2.04 years. Collect and count surgical-related indicators, preoperative and postoperative radiologic findings, incision visual analogue scale (VAS), lumbar and leg pain VAS, lumbar Japanese Orthopaedic Association (JOA) and Oswestry disability index (ODI) scores of all patients.
Result
A series of surgical indicators (including the operation time, the quantity of bleeding and postoperative hospital stay) in the endoscopy group was significantly lower than that in the control group (p < 0.05). The incision VAS score in the endoscopy group was also significantly lower than that in the control group at each time after surgery (p < 0.05). Besides, compared with the control group, in the endoscopy group, the leg pain VAS score and lumbar ODI score after surgery were significantly decreased (p < 0.05). Compared with the control group, in the endoscopy group, the lumbar JOA score was significantly higher (p < 0.05).
Conclusion
Percutaneous endoscopic technique is a small trauma, quick recovery, safe and effective minimally invasive surgery for patients with lumbar spinal stenosis secondary to ligamentum flavum hypertrophy.
Clonality of Enterococcus Faecalis Isolates in Stool and Urine Samples of Patients with Community-Acquired Urinary Tract Infection, and its Link with Virulence Gene Determinants and Antimicrobial Resistance Phenotypes
Authors: Ghalavand Zohreh , Alebouyeh Masoud , Ghanati Kiandokht , Azimi Leila , rashidan marjan 
Published: May 2020

Abstract
Background
Community-acquired urinary tract infection (CA-UTI) could be caused through endogenous or exogenous routes. To show this relationship, we investigated molecular fingerprints and genotypes of paired Enterococcus faecalis isolates from the urine of symptomatic patients and their fecal samples.
Results
Out of the studied patients, 63 pairs of E. faecalis isolates were obtained simultaneously from their urine and feces samples. All the strains were sensitive to vancomycin, linezolid, nitrofurantoin, and daptomycin (MIC value: ≤4 µg/ml), whileresistance to tetracycline (Urine: 88.9%; stool: 76,2%) and minocycline (Urine: 87.3%, stool: 71.4%) wasdetected in most of them. The most common detected virulence genes were included efbA, ace, and gelE. RAPD-PCR and PFGE analyses showed same patterns of molecular fingerprints between paired of the isolates in 26.9% and 15.8% of the patients, respectively.
Conclusions
Similarity of E. faecalis strains between the urine and feces samples confirmed the occurrence of endogenous infection via contamination with colonized bacteria in the intestinal tract. Carriage of a complete virulence genotype in the responsible strains was statistically in correlation with endogenous UTI, which show their possible involvement in pathogenicity of uropathogenic E. faecalis strains.
High-level expression of CXCL1/GROα in uterine cervix cancer is linked to advanced stage and facilitates tumor cell malignant processes in an autocrine/paracrine manner
Authors: Man XiaXia , Yang XiaoLin , Wei ZhenTong , Tan YuYing , Li WanYing , Jin HongJuan , Wang Baogang 
Published: May 2020

Abstract
Background

It has previously accepted that several types of human cancer constitutively express CXCL1, which may implicated in various aspects of tumors. Here, we sought to assess the expression and of CXCL1 in human uterine cervix cancer (UCC) and its potential role and mechanism in UCC.
Methods

CXCL1 protein expression in a uterine cervical tissue microarray was assessed by immunohistochemistry. The roles of CXCL1 on HeLa UCC cells were detected by CCK-8, transwell and apoptosis assays. Western blotting and ERK1/2 blocker PD98059 were utilized to explore whether ERK signal was implicated in CXCL1-mediated UCC processes.
Results

CXCL1 was expressed by HeLa, PHM1-41 cells as well as cervical tissues, in which UCC tissues showed an obviously high level of CXCL1 compared with non-cancerous tissues. Additionally, of the cancer tissues, CXCL1 high-level expression was notably relevant to poor clinical stages. In vitro, the growth and migration of HeLa cells were enhanced in the presence of exogenous CXCL1. Gain-function assays revealed that CXCL1 overexpression promoted growth and migration response to HeLa cells via autocrine/paracrine manners. Moreover, CXCL1 also led to the inhibition of apoptosis in HeLa cells. Finally, CXCL1 overexpression in HeLa cells influenced the expression of ERK signal-related genes including ERK, p-ERK, cyclin D1 and Bax, and cell malignant behaviors derived from CXCL1 overexpresion were further interrupt in the presence of PD98059.
Conclusion

Our findings provided the potential roles of CXCL1 as a promoter and the novel understanding of the functional relation of CXCL1 with ERK signal pathway in UCC.
Metabolic genes show excellent prognostic ability for clear cell renal cell carcinoma
Authors: Ji Chengjian , Wang Yichun , Yao Liangyu , Luan Jiaochen , Cong Rong , Wang Yamin , Qin Yuan 
Published: May 2020

Abstract
Background

Renal cell carcinoma (RCC) is one of the major malignant tumors of the urinary system, with a high mortality rate and a poor prognosis. Clear cell renal cell carcinoma (ccRCC) is the most common subtype of RCC. Although the diagnosis and treatment methods have been significantly improved, the incidence and mortality of ccRCC are high and still increasing. The occurrence and development of ccRCC are closely related to the changes of classic metabolic pathways. This article aims to explore the relationship between metabolic genes and the prognosis of patients with ccRCC.
Patients and methods:

Gene expression profiles of 63 normal kidney tissues and 446 ccRCC tissues from TCGA database and gene expression profiles of 39 ccRCC tissues from GEO database were used to obtain differentially expressed genes (DEGs) in ccRCC. Through the the KEGG gene sets of GSEA database, we obtained metabolic genes (MGs). Univariate Cox regression analysis was used to identify prognostic MGs. Lasso regression analysis was used to eliminate false positives because of over-fitting. Multivariate Cox regression analysis was used to established a prognostic model. Gene expression data and related survival data of 101 ccRCC patients from ArrayExpress database were used for external validation. Survival analysis, ROC curve analysis, independent prognostic analysis and clinical correlation analysis were performed to evaluate this model.
Results

We found that there were 479 abnormally expressed MGs in ccRCC tissues. Through univariate Cox regression analysis, Lasso regression analysis and multivariate Cox regression analysis, we identified 4 prognostic MGs (P4HA3, ETNK2, PAFAH2 and ALAD) and established a prognostic model (riskScore). Whether in the training cohort, the testing cohort or the entire cohort, this model could accurately stratify patients with different survival outcomes. The prognostic value of riskScore and 4 MGs was also confirmed in the ArrayExpress database. Results of GSEA analysis show that DEGs in patients with better prognosis were enriched in metabolic pathways. Then, a new Nomogram with higher prognostic value was constructed to better predict the 1-year OS, 3-year OS and 5-year OS of ccRCC patients. In addition, we successfully established a ceRNA network to further explain the differences in the expression of these MGs between high-risk patients and low-risk patients
Conclusion

We have successfully established a risk model (riskScore) based on 4 MGs, which could accurately predict the prognosis of patients with ccRCC. Our research may shed new light on ccRCC patients' prognosis and treatment management.
RNF43 and PWWP2B inhibit cancer cell proliferation and are predictive or prognostic biomarker for FDA-approved drugs in patients with advanced gastric cancer
Authors: Sohn Sung-Hwa , Sul Hee Jung , Kim Bohyun , Kim Hyeong Su , Lim Hyun , Kang Ho Suk , Soh Jae Seung , Kim Kab Choong , Cho Ji Woong , Seo Jinwon , Koh Youngho , Zang Dae Young 
Published: May 2020

Abstract
Background

The characterization of gastric cancer has necessitated the development of new therapeutics as well as the identification of prognostic markers to predict the response to novel drugs. In our study, we have performed RNA sequencing analysis on gastric cancer tissues that decreased levels of RNF43 and PWWP2B were significantly associated with recurrence (11/11, 100%, P < 0.001). Therefore, we investigated the screening of 1,449 FDA-approved drugs in HAP1, HAP1 RNF43 KO and HAP1 PWWP2B KO cells.
Methods

We demonstrated that RNF43 KO and PWWP2B KO cells showed significantly increased proliferation and migration abilities. Next, we investigated the inhibitory effects of 1,449 drugs in HAP1, HAP1 RNF43 KO and HAP1 PWWP2B KO cells.
Results

Among these FDA-approved drugs, three drugs (docetaxel trihydrate, pelitinib and uprosertib) showed strong inhibitory effects in RNF43 KO cells and PWWP2B KO cells. In RNF43 and PWWP2B down-regulated MKN45 xenograft model, tumor volumes were significantly reduced in the docetaxel trihydrate, uprosertib or pelitinib-treated group. Our data demonstrated that RNF43 and PWWP2B are a biomarker that predict recurrence of gastric cancer.
Conclusion

Our findings suggest that docetaxel trihydrate, uprosertib and pelitinib could be used as novel therapeutic agents for the prevention and treatment of gastric cancer with an aberrant decrease in RNF43 and PWWP2B expression.
Difference in the Foot Intersegmental Coordination Pattern Between Female Lacrosse Players With and Without a History of Medial Tibial Stress Syndrome: a Cross-sectional Study
Authors: Akuzawa Hiroshi , Oshikawa Tomoki , Nakamura Koji , Kubota Ren , Takaki Norifumi , Matsunaga Naoto , Kaneoka Koji 
Published: May 2020

Abstract
Background
Medial tibial stress syndrome is a common sports related injury. Altered foot kinematics can be a risk factor for the injury. Since foot segments can move independently, intersegment coordination is important for proper foot function. This study aimed to compare the foot intersegmental coordination pattern and single segment kinematics between women lacrosse players with and without a history of medial tibial stress syndrome during drop jump.
Methods
Nine players with a medial tibial stress syndrome history and 12 players with no history were enrolled. Foot kinematics, including angle at landing and peak angle and excursion at the rearfoot, midfoot, and forefoot during single-leg drop jumps were analyzed. Each segment motion data from landing to leaping was time-scaled to 100% to analyze the intersegmental coordination with a modified vector coding technique. Instant intersegmental coordination of every 1% was classified into four patterns (in-phase, two segments rotate in the same direction with similar amplitudes; anti-phase, two segments rotate in opposite directions; proximal phase, proximal segment dominantly rotates in the same direction compared to the distal segment; and distal phase, distal segment dominantly rotates in the same direction compared to the proximal segment). The percentage of intersegmental coordination pattern and kinematics in each segment were compared between the groups using the Student’s t test.
Results
Groups with a history of medial stress syndrome showed a significantly higher percentage of proximal phase between the rearfoot and midfoot in the sagittal (Mean ± SD; history, 52.0 ± 18.5%, no history, 29.3 ± 16.7%; p = 0.005) and coronal planes (history, 43.0 ± 26.1%, no history, 15.9 ± 9.1%; p = 0.008). Dorsiflexion excursion (history, 34.8 ± 4.7°, no history, 29.6 ± 2.1°; p = 0.002) were significantly larger in a history of medial tibial stress syndrome group compared to no history group.
Conclusions
Rearfoot dominant motion pattern relative to the midfoot may be related to medial tibial stress syndrome. Intersegmental coordination analysis may be useful for detecting abnormal foot coordination patterns.
The effects of composition, temperature and hydrostatic pressure on phase transition behaviors in (Pb1-1.5xLax)(Zr0.8Ti0.2)O3 ceramics
Authors: Xie Meng , Bao Yizheng , Nie Hengchang , Cao Fei , Dong Xianlin , Wang Genshui 
Published: May 2020

Abstract
In this work, (Pb1 − 1.5xLax)(Zr0.80Ti0.20)O3 (abbreviated as PLZT, x = 0.01, 0.03, 0.04, 0.06, 0.07) ceramics are designed on the base of chemical composition modification and prepared by solid-state reaction. The effect of composition, temperature, and hydrostatic pressure on ferroelectric-antiferroelectric (FE-AFE) phase transition is investigated. It is obtained that phase transition from ferroelectric rhombohedral phase to antiferroelectric tetragonal phase as a function of La3+ doping content, especially, the PLZT ceramics of x = 0.04, 0.06, and 0.07 are the coexistence of FE-AFE phase. It is also found the FE-AFE phase transition driven by increased temperature in poled PLZT ceramics (x = 0.04, 0.06). Furthermore, static charges density (Pr) of PLZT (x = 0.04, 0.06) are decreased from 29.11 µC/cm2 and 31.52 µC/cm2 to 19.76 {\mu }C/cm2, 6.45 {\mu }C/cm2 under 400 MPa hydrostatic pressure due to the pressure-induced FE-AFE phase transition. The depolarization rates are 32.12% and 79.54%, respectively. Meanwhile, the phase diagram of (Pb1 − 1.5xLax)(Zr0.80Ti0.20)O3 ceramics is acquired roughly. These results provide guidance for the engineering application of (Pb1 − 1.5xLax)(Zr0.80Ti0.20)O3 ceramics.
Socio-economic impact of Jatropha-based biofuel promotion on rural livelihoods in northern Tanzania
Authors: Olotu Moses 
Published: May 2020

Abstract
Background In recent years, there have been global concerns regarding the continuous use of non-renewable fossil fuels as the major source of energy. The utilization of non-renewable fossil fuels has negative effects on global warming and climate change. So far, efforts to curb the situations have tended to focus on promoting alternative renewable energy sources such as the use of biofuels to cut down emissions of green house gases. However, the production of biofuel crops has become an issue of concern in many developing countries. This is because the expansion of land for biofuel crops cultivation is directly linked to food security and the livelihoods of rural communities. The present study, therefore, aimed to evaluate the contribution of non-food biofuel crops (Jatropha curcas) to the improvement of rural household livelihoods and its impacts on food security. A survey was conducted in 84 households in Arumeru and Siha Districts in the northern Tanzania.Results The results revealed that the introduction of Jatropha as a cash crop had no significant impact on the rural livelihoods since the five capitals of the framework have not optimally been utilised despite their presence. It was further revealed that Jatropha cultivation has not affected food security. This is contrary to fears that biofuels could generally have a negative impact on food security especially in developing countries. The crop had not been regarded as a cash crop or a biofuel; thus, a very little percentage of land owned by the respondents had been allocated for its cultivation. The importance of Jatropha in the study area has yet to be recognised and given the special attention it requires.Conclusions It can be concluded that, the current situation does not indicate any significant impact of Jatropha cultivation on food security. However, it has little contribution to livelihood improvement of the smallholder farmers. Therefore, research on the improvement of Jatropha productivity under optimum conditions and pricing should be further undertaken in different areas of the country.