bluuebunny/crossref_metadata_2025_split huggingface dataset:
Knockdown of ZNF280A inhibits the development and progression of ovarian cancer
Authors: Zhu Dawei , Gu Xing , Li Li , Chen Xuebing , Huang Jie 
Published: May 2020

Abstract
Background

Ovarian cancer (OC) is one of the leading causes of death from gynecological malignancies worldwide. Abnormal expression of zinc finger proteins has been extensively reported to be involved in malignant progression in a variety of cancers. However, clinical significance and biological roles of ZNF280A in the field of OC are poorly known.
Methods

In this study, we demonstrated that ZNF280A was highly expressed in OC tissues compared with adjacent normal tissues. Further, ZNF280A was positively associated with clinical staging, infiltration, lymphatic metastasis, metastasis, and tumor recurrence of OC patients. Additionally, data of in vitro experiments indicated that knockdown of ZNF280A by its shRNA dramatically reduced the proliferation and migration ability of OC cells, while enhancing the cell apoptosis.
Results

It was also verified by animal experiments that ZNF280A silencing would affect the growth of OC in vivo. Our study investigated the involvement of ZNF280A in the prognosis, progression and metastasis of OC.
Conclusions

Therefore, ZNF280A was identified as an optional prognostic factor in OC patients and can be used as a potential therapeutic target for the treatment of OC.
Integrative analysis of the characteristic of lipid metabolism-related genes for the prognostic prediction of hepatocellular carcinoma
Authors: Chen Wenbiao , Zeng Jun , Ou Minglin , Tang Donge 
Published: May 2020

Abstract
Background

Dysregulation of lipid metabolism has been implicated in the progression of hepatocellular carcinoma (HCC). We therefore investigated the molecular characteristics of lipid-metabolism-related genes for the prognostic prediction of HCC.
Methods

Multi-dimensional bioinformatics analysis was conducted to comprehensively analyzed the lipid metabolism-related genes (IMRG) and construct the prognostic prediction signature.
Results

A total of 770 HCC patients and their corresponding 776 IMRGs were downloaded from three databases. The HCC patients were classified into 2 molecular clusters, which were associated with overall survival, clinical characteristics, and immune cells. The biological function of the differentially expressed IMRGs in the 2 clusters showed that the genes were associated with tumor-related metabolism pathways. A 6 IMRGs signature (6-IS), including FMO3, SLC11A1, RNF10, KCNH2, ME1, and ZIC2 were established for HCC prognostic prediction, which was found to be an independent prognostic factor. Performance of the 6-IS prognostic signature was verified in a validation set and compared with an external data set. Results revealed that the 6-IS signature could effectively predict the prognosis of patients with HCC.
Conclusion

This study provides new insights into the role of IMRG in the pathogenesis of HCC and presents a novel signature 6-IS to predict the prognosis of HCC.
LncRNA XIST acts as a microRNA-520 sponge to regulate the cisplatin resistance in NSCLC cells by mediating BAX through ceRNA network
Authors: Liu Ting-Ting , Li Rui , Liu Xiao , Zhou Xi-Jia , Huo Chen , Li Jian-Ping , Qu Yi-Qing 
Published: May 2020

Abstract
Background

In recent years, LncRNA acts as a member of competing endogenous RNA (ceRNA), playing an important role in drug resistance of lung cancer. The aim of this study was to identify potential biomarkers about cisplatin resistant lung cancer cells using a comprehensive ceRNA network.
Methods

GSE6410 (GPL-201) analyzed gene expression changes about cispaltin resistance in A549 NSCLC cells. GSE43249 (GPL-14613) included noncoding RNA expression profiling derived from the cisplatin resistant A549 lung cells. GEO2R, an online analysis tool, analyzed the differentially expressed mRNAs and miRNAs (DEmRNAs and DEmiRNAs). To explore the functional enrichment implication of differentially expressed mRNAs, we used the GO and KEGG pathway analysis. Through miRDB、Targetscan、Starbase、miRWalk, we found targeted miRNAs. The Kaplan-Meier curve method was used to show clinical survival analysis of targeted RNAs (P < 0.05). The Starbase database predicted potential lncRNAs mediated targeted miRNAs. Eventually, the novel ceRNA network of lncRNAs, miRNAs, mRNA was constructed by cytoscape3.7.2.
Results

118 differentially expressed mRNAs were the basis of the mediated ceRNA network. DAVID and Kaplan-Meier picked out BAX, an apoptosis regulator. Venn Diagram demonstrated 8 miRNAs commomly regulating Bax. Starbase predicted lncRNA XIST mediated miRNAs. Finally, lncRNA XIST maybe a useful biomarker regulating cisplatin resistance in lung cancer cells.
Conclusions

LncRNA XIST competitively bound to miRNA 520 in the regulation of cisplatin resistance by BAX, participating apoptosis in the p53 signaling pathway.
Chronic Kidney Disease and associated factors among HIV/AIDS Patients on HAART at University of Gondar Referral Hospital, Northwest Ethiopia
Authors: Manaye Gizachew Ayele , Abateneh Dejene Derseh , Asmare Wondwossen Niguse 
Published: May 2020

Abstract
Background

In developing countries, both opportunistic infections and chronic diseases account a high HIV associated mortality and morbidity. Chronic kidney diseases (CKD) associated with HIV infection has got increased attention in sub-Saharan Africa as a result of the high HIV prevalence and due to the late diagnosis and initiation of HAART. Thus, this study was conducted to assess CKD and associated factors among HIV patients on HARRT in Northwest Ethiopia.
Methods

A hospital-based cross-sectional study with a secondary data review was conducted from February 01 to April 30, 2017, at the University of Gondar Referral Hospital, Northwest Ethiopia. The study participants were selected using a systematic random sampling technique. Socio-demographic and clinical data were collected using a semi-structured questionnaire by trained nurses. Venous blood and urine specimen was collected for serum creatinine and urine protein determination respectively. Glomerular filtration rate was estimated using the CKD–EPI estimator. Data were entered into SPSS version 20 for analysis. Bivariate and multivariate logistic regression was employed and p-value < 0.05 was considered statistically significant.
Results

A total of 336 HIV patients on HARRT participated in the study. The mean (SD) age of the participants was 39.7 (± 9.7) years. The study participants were on HAART with an average of 7.5 (± 3) years. Before ART initiation, the majority of patients had WHO clinical stage II and III, 251 (74.7%), CD4 + T cell count < 200 cells/mm3, 221 (65.8%).The overall prevalence of CKD was 16.1%. About 27 (8.0%), 16 (4.8%), 11 (3.3%) of the participants had stage 1 and stage 2 CKD and chronic renal failure (stage 3a-5) respectively. With multivariate logistic regression analysis being male (AOR = 2.05 (1.03–4.09), p = 0.04, occupation merchant (AOR = 2.91(1.00-8.48),p = 0.049)and viral load ≥ 1000 copies/mm3 (AOR = 3.1 (1.38-7.00), P < 0.01) were significantly associated with CKD.
Conclusions

The prevalence of CKD among HIV patients on HARRT is high. Being male, merchant and viral load ≥ 1000 copies/mm3 were associated factors of CKD. Patients should be regularly monitored and screened for early diagnosis and management of CKD. Those patients with high viral load and male patients should be closely followed.
Australasian Interstitial Lung Disease Registry (AILDR): Objectives, Design and Rationale of a Bi-National Prospective Database
Authors: Moore Irene , Wrobel Jeremy , Rhodes Jessica , Lin Qi , Webster Susanne , Jo Helen , Troy Lauren , Grainge Christopher , Glaspole Ian , Corte Tamera J 
Published: May 2020

Abstract
Background:
Interstitial Lung Disease (ILD) is a group of respiratory conditions affecting the lung interstitium often associated with progressive respiratory failure. There is increasing recognition of the need for improved epidemiological data to help determine best practice and improve standardisation of care. The Australasian ILD Registry (AILDR) is a bi-national registry of patients with all ILD subtypes designed to establish a clinically meaningful database reflecting real world practice in Australasia with an objective to improve diagnostic and treatment pathways through research and collaboration.
Methods:
AILDR is a prospective observational registry recruiting patients attending ILD clinics at centres around Australia and New Zealand. Core and non-core data are stored on a secure server. The pilot phase was launched in 2016 consisting of four sites in Australia. Currently in its second phase a further 16 sites have been recruited, including three in New Zealand.
Results:
A total of 1061 participants were consented during the pilot phase. Baseline data demonstrated a mean age 68.3 ± 12.5 (SD) years, mean FVC (%predicted) 79.1 ± 20.4 (SD), mean DLCO (%predicted) 58.5 ± 17.9 (SD) and nadir exertional SpO2 (%) 91 ± 6.9 (SD). Idiopathic pulmonary fibrosis (31%) and connective-tissue disease related ILD (21.7%) were the two most common subtypes. Baseline demographics and physiology were not significantly different across the four centres.
Conclusion:
AILDR is an important clinical and research tool providing a platform for epidemiological data that will prove essential in promoting understanding of a rare cohort of lung disease and provide foundations for our aspiration to standardise investigation and treatment pathways of ILD across Australasia.
Transport and Chronic Injuries of Cell Phone use on Human Health: A Systematic Analysis of Epidemiological Evidence
Authors: Cao Xinxi , Xu Chenjie , Hou Yabing , Yang Hongxi , Li Shu , Gao Ying , Jia Peng , Wang Yaogang 
Published: May 2020

Abstract
Background Cell phone use brought convenience to people, but using phones for a long period of time or in the wrong way and with a wrong posture might cause damage to the human body. This study was designed to assess the impact of cell phone use on transport and chronic injuries.Methods Studies were systematically searched in four database and relevant reviews were searched to identify additional studies. A total of 41 studies met the inclusion criteria.Results Cell phone users were at a higher risk for transport injuries (RR: 1.37, 95%CI: 1.22−1.55), long-term use of cell phones increased the transport injury risk to non-use or short-term use (RR: 2.10, 95% CI: 1.63−2.70). Neoplasm risk caused by cell phone use was 1.07 times that of non-use (95% CI: 1.01−1.14); Compared with non-use, cell phone use had a higher risk of eye disease, with a risk of 2.03 (95% CI: 1.27−3.23), the risk of mental disease was 1.26 (95% CI: 1.17−1.35), the risk of neurological disorder was 1.16 (95% CI: 1.02−1.32), and a pooled risk of other chronic injuries, was 1.20 (95% CI: 0.98−1.59). Subgroup analyses found that motor crashes had significantly increased (OR: 1.25; 95%CI: 1.18−1.32), as well as the risk for hearing problems (OR: 4.54; 95%CI: 3.29−5.80), headaches (OR: 1.25; 95%CI: 1.18−1.32), and abnormal biochemical indicators (OR: 0.51; 95%CI: 0.04−0.99).Conclusions Cell phone use at inappropriate situations has a negative impact on the human body. Therefore, it is necessary to use cell phones correctly and reasonably.
Effect of different kinds of SiC fibers on microwave absorption and mechanical properties of SiCf/SiC composites
Authors: Ren Zhaowen , Zhou Wancheng , Qing Yuchang , Duan Shichang , Wen Qinlong , Zhou Yingying , Li Yanyu 
Published: May 2020

Abstract
The SiC fibers are essential for designing microwave absorption and mechanical properties of multifunctional composites. In this study, SLF, KD-II and KD-S SiC fibers were used to fabricate SiC f /SiC composites. The SLF SiC fibers are composed by amorphous SiOC. The KD-II and KD-S SiC fibers exhibit higher crystallizations and free carbon content. The conductivity of SLF, KD-II and KD-S SiC fibers are 0.0127, 1.184 and 0.1316 S/cm, respectively. The flexural strength of SLF, KD-II and KD-S SiC f /SiC composites are 147.77, 322.57 and 248.16 MPa, respectively. The microwave absorption property of the SLF SiC f /SiC composites can be obtained over -25 dB with a thickness of 2.3 mm and the effective absorption bandwidth (EAB) below -10 dB reaches 3.72 GHz with the thickness of 2.7 mm. In contrast, the KD-II SiC f /SiC composites only reach -3.6 dB in the whole X band when the thickness varies from 2 to 2.9 mm. KD-S SiC f /SiC composites can be obtained over -9dB with the thickness of 2mm and the EAB below -7 dB reaches 4.12 GHz with a thickness of 2.2 mm. The mechanisms of mechanical, microwave absorption and penitential applications for SiC f /SiC composites are also discussed.
Imaging anatomy and clinical significance of percutaneous endoscopic transforaminal oblique fixation from posterior corner in lumbar spine
Authors: Chen Feifei , Liu Xiaoyang , Sun Jianmin , Xin Jun , Su Cheng , Cui Xingang 
Published: May 2020

Abstract
BackgroundPercutaneous endoscopic transforaminal lumbar interbody fusion (PE-TLIF) has been widely discussed due to its advantages of less trauma, less bleeding, quick recovery, high safety, and relatively fewer complications, as well as other adverse factors such as incomplete decompression, steep learning curve, low fusion rate, and high radiation risk. It can keep the posterior structure of spine intact to the greatest extent, ensure the stability of spine after surgery, and achieve decompression with minor trauma. However, posterior percutaneous pedicle screws are often needed for fusion and fixation after decompression, and additional posterior trauma, postural changes and anesthesia methods are often required. Interbody fixation and fusion are often independent and not one-stop completion. The authors consider whether the percutaneous spinal endoscopy can be used to achieve complete decompression and fusion under a single minimally invasive channel, while achieving one-stop endoscopic decompression, fusion and fixation. The purpose of this paper is to provide the anatomic feasibility for oblique fixation by measuring the imaging anatomic parameters, especially to provide the anatomic basis for the design of new endoscopic lumbar interbody fusion cage.Methods Sixty volunteers (22 men and 38 women) who underwent lumbar CT scans were collected and sent to the GEAW4.4 workstation. The distances from posterior corner in the lumbar spine to the corresponding targets of the contralateral anterior corner and the included angles between each path line in sagittal and axial plane were measured and analyzed statistically.Results In the medium group, PC path was the shortest, PA path and PB path had little difference (P=0.123), with no statistical significance. In the full-length group, PF path was the shortest, and there was no significant difference between PD path and PE path (P =0.177). PE was the optimal path. The included angles a1, a2, a3, b1, b2, and b3 in sagittal plane and c1, c2 and c3 in axial plane were significantly different (P=0.000), namely, a1 >a2>a3, b1>b2>b3, and c1<c2<c3. Conclusions This study provides anatomic feasibility for percutaneous endoscopic transforaminal oblique fixation from posterior corner in lumbar spine and particularly provides anatomic basis for the design of new endoscopic lumbar interbody fusion cage.
Identification of an autophagy-related gene expression prognostic model in endometrial carcinoma patients
Authors: Jiang Pinping , Sun Wei , Shen Ningmei , Wang Qiang , Wang Shouyu , Fu Shilong 
Published: May 2020

Abstract
Background

Autophagy, as a lysosomal degradation pathway, has been reported to be involved in various pathologies, including cancer. However, the expression profiles of autophagy-related genes (ARGs) in endometrial cancer (EC) remain poorly understood.
Methods

In this study, we analyzed the expression of MRGs using The Cancer Genome Atlas (TCGA) data to screen differentially expressed MRGs (DE-MRGs) significantly correlated to EC patients’ prognosis. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of DE-MRGs were investigated. LASSO algorithm and Cox regression analysis were performed to select MRGs closely related to EC patients’ outcomes. A prognostic signature was developed and the efficacy were validated in part of and the entire TCGA EC cohort. Moreover, we developed a comprehensive nomogram including the risk model and clinical features to predict EC patients' survival probability.
Results

Ninety-four ARGs significantly dysregulated in EC samples compared with the normal control samples. Functional enrichment analysis showed these differentially expressed ARGs (DE-ARGs) were highly enriched in apoptosis, P53 signaling pathway, and various cancer development. Among the 94 DE-ARGs, we subsequently screen out four-ARGs closely related to EC patients outcomes, which are ERBB2, PTEN, TP73 and ARSA. Based on the expression and coefficiency of 4 DE-ARGs, we developed a prognostic signature and further validated its efficacy in part of and the entire TCGA EC cohort. The four ARGs signature was independent of other clinical features, and was proved to effectively distinguish high- or low-risk EC patients and predicted patients' OS accurately. Moreover, the nomogram showed the excellent consistency between the prediction and actual observation in terms of patients' 3- and 5-year survival rates.
Conclusions

It was suggested that the ARG prognostic model and the comprehensive nomogram may guide the precise outcome prediction and rational therapy in clinical practice.
Hypertension patients infected with COVID- 19 undergo a longer period of negative conversion:A retrospective study
Authors: Xiao Bing , Fu Dou , Yang Wei , Zou Can , Zhang Bing , Li Xia , Zeng Jing , Li YuanFa , Chen Gong , Sha Junping 
Published: May 2020

Abstract
Purpose Previous studies have showed that age,sex and comorbidities might be associated with severity of patients infected with COVID-19 ,which endangers public health worldwide rapidly.The characteristics and length of negative conversion of Non-severe COVID-19 patients with or without hypertension is limited.This study aims to assess whether non-severe COVID-19 patients with hypertension undergone more longer period of negative conversion .Methods This single-center, retrospective study was performed in Xiantao first People’s Hospital Affiliated to Yangtze University(xiantao, hubei,China) by using medical records.Non-severe COVID-19 patients with a history of hypertension From January 23 to February15 were enrolled as group A. A Control group(group B) was matched (1:1) according to age,sex and the admission date.Data on clinical records, laboratory results, and radiological tests was collected. we conducted all analyses with SPSS software(22.0).Results We enrolled 24 and 24 Non-severe COVID-19 patients with and without hypertension, respectively. The most common symptoms were fever and cough in both groups.The frequency of fatigue is more in patients with hypertension.The levels of AST,ALT and CRP were higher in group A.The mean periods of negative conversion for COVID-19 virus were 17 days(SD:5.5) and 15 days(SD:3.6)for patients with and without hypertension(P=0.021).The mean hospitalization periods were 16.8 days (SD:5.6) and 13.7 days (SD:3.8) , respectively (p=0.083) .Conclusion Non-severe COVID-19 patients with hypertension undergone a longer negative conversion for COVID-19 virus and spent more time on clearing COVID-19 virus.
Beagle dog 90-day oral toxicity study of a novel coccidiostat – ethanamizuril
Authors: Zhang Keyu , Wang Xiaoyang , Wei Shuya , Wang Chunmei , Wang Mi , Liu Yingchun , Zhang Lifang , Xue Feiqun , Tang Shusheng 
Published: May 2020

Abstract
Background: Ethanamizuril is a novel triazine compound that exhibits remarkable anticoccidial activity in poultry. Due to its pharmacological properties, this study was to evaluate the 90-day subchronic toxicity of feeding ethanamizuril fed to beagle dog. Methods: The subchronic toxicity study was conducted in beagle dogs administered ethanamizuril by diet at doses of 12, 60 or 300 mg/kg/day for 90 days, respectively. Results: Ethanamizuril was well tolerated at low and middle dosages and there were no test article-related effects on survival, clinical observations, clinical pathology parameters, organs weight, macroscopic or microscopic evaluations. Test article-related changes were limited to effects on food consumption and histologic changes of kidneys in the 300 mg/kg/day group in both genders. However, the characteristic toxicities of ethanamizuril are recoverable in kidneys. Conclusions: Therefore, the no-observed-adverse-effect level (NOAEL) was considered to be 60 mg/kg/day, the middle dosage level tested. These results add to the safety database for ethanamizuril with potential for use as a novel coccidiostat.
Bacillus subtilis spore vaccines displaying protective antigen induce functional antibodies and protective potency
Authors: Oh Yeonsu , Kim Jung Ae , Kim Chang-Hwan , Choi Soo-Keun , Pan Jae-Gu 
Published: May 2020

Abstract
Background: Bacillus anthracis is the causative agent of anthrax, a disease of both humans and various animal species, and can be used as a bioterror agent. Effective vaccines are available, but those could benefit from improvements, including increasing the immunity duration, reducing the shot frequency and adverse reactions. In addition, more sophisticated antigen delivery and potentiation systems are urgently required. The protective antigen (PA), one of three major virulence factors associated with anthrax was displayed on the surface of Bacillus subtilis spores, which is a vaccine production host and delivery vector with several advantages such as a low production cost, straightforward administration as it is safe for human consumption and the particulate adjuvanticity. Mice were immunized orally (PO), intranasally (IN), sublingually (SL) or intraperitoneally (IP) with the PA displaying probiotic spore vaccine. Clinical observation, serological analysis and challenge experiment were conducted to investigate the safety and efficacy of the vaccine. Results: A/J mice immunized with the PA spore vaccine via PO, IN, SL, and IP were observed to have increased levels of active antibody titer, isotype profiles and toxin neutralizing antibody in sera, and IgA in saliva. The immunized mice were demonstrated to raise protective immunity against the challenge with lethal B. anthracis spores. Conclusions: In this study, we developed a B. subtilis spore vaccine that displays the PA on its surface and showed that the PA-displaying spore vaccine was able to confer active immunity to a murine model based on the results of antibody isotype titration, mucosal antibody identification, and a lethal challenge experiment.