bluuebunny/crossref_metadata_2025_split huggingface dataset:
Inside environmental Clostridium perfringens genomes: antibiotic resistance genes, virulence factors and genomic features
Authors: Fourie Johannes Cornelius Jacobus , Bezuidenhout Cornelius Carlos , Sanko Tomasz Janusz , Mienie Charlotte , Adeleke Rasheed 
Published: May 2020

Abstract
Until recently, research has focused on Clostridium perfringens in clinical settings without considering environmental isolates. In this study, environmental genomes were used to investigate possible antibiotic resistance and the presence of virulence traits in C. perfringens strains from raw surface water. In silico assembly of three C. perfringens strains, DNA generated almost complete genomes setting their length ranging from 3.4 to 3.6 Mbp with GC content of 28.18%. An average of 3,175 open reading frames was identified, with the majority associated with carbohydrate and protein metabolisms. The genomes harboured several antibiotic resistance genes for glycopeptides, macrolide–lincosamide–streptogramin B, β-lactam, trimethoprim, tetracycline and aminoglycosides and also the presence of several genes encoding for polypeptides and multidrug resistance efflux pumps and 35 virulence genes. Some of these encode for haemolysins, sialidase, hyaluronidase, collagenase, perfringolysin O and phospholipase C. All three genomes contained sequences indicating phage, antibiotic resistance and pathogenic islands integration sites. A genomic comparison of these three strains confirmed high similarity and shared core genes with clinical C. perfringens strains, highlighting their health security risks. This study provides a genomic insight into the potential pathogenicity of C. perfringens present in the environment and emphasises the importance of monitoring this niche in the future.
Solar inactivated Vibrio cholerae induces maturation of JAWS II dendritic cell line in vitro
Authors: Ssemakalu Cornelius Cano , Ubomba-Jaswa Eunice , Motaung Keolebogile Shirley Caroline Mamotswere , Pillay Michael 
Published: May 2020

Abstract
Solar disinfection (SODIS) has been shown to reduce the risk associated with the contraction of water borne diseases such as cholera. However, little or no research has been undertaken in exploring the role played by the immune system following the consumption of solar inactivated water pathogens. This study investigated the potential for solar inactivated Vibrio cholerae to induce the maturation of dendritic cells in vitro. Dendritic cells are professional antigen presenting cells found in mammals. However, only in their mature form are dendritic cells able to play their role towards a long lasting immune response. Three strains of V. cholerae were solar irradiated for 7 hours. Thereafter, the solar irradiated, non-solar irradiated, phosphate buffered saline prepared and heat/chemically inactivated cultures of V. cholerae as well as lipopolysaccharide and cholerae toxin-β subunit were used to stimulate immature dendritic cells. After 48 hours, the dendritic cells were assessed for the expression of CD54, CD80, CD83, CD86, MHC-I and MHC-II cell surface markers. Results show that solar inactivated V. cholerae was able to induce maturation of the dendritic cells in vitro. These findings suggest that there may be an immunological benefit in consuming SODIS treated water.
Distribution and characteristics of geosmin and 2-MIB-producing actinobacteria in the Han River, Korea
Authors: Lee Eun-Sook , Kim Yu-Na , Kim Sae-Bom , Jung Jong-Soon , Cha Yeoung-Seop , Kim Bog-Soon 
Published: May 2020

Abstract
Actinobacteria can be one of the causes of earthy and musty odors in drinking water. In this study, the distribution and odor producing ability of actinobacteria isolated in the Han River as a source of tap water were investigated. Actinobacteria were detected in low concentration from December to February and this gradually increased in March and April. The number of actinobacteria detected was particularly high in April (63 CFU/mL), July (45 CFU/mL), and October (39 CFU/mL) due to the influence of rainfall. Actinobacteria with geosmin-producing genes were detected mainly in March and July. In contrast, actinobacteria with 2-MIB-producing genes were detected mainly in October. There was a difference in the time when actinobacteria with the geosmin and 2-MIB-producing gene were highly detected in the river. Also, the types of actinobacteria with the geosmin and 2-MIB-producing gene were different. More than 70% of the geosmin inducer gene was isolated in Streptomyces, but the 2-MIB inducer gene was detected in various genera of actinobacteria as well as Streptomyces. The detection of odorous substances in March and October when cyanobacteria were not detected, or the detected number was low, suggested that actinobacteria could be a cause of odor inducers in the Han River.
Effects of temporary external voltage on the performance and community of microbial fuel cells
Authors: Guo Jing , Cheng Jianping , Wang Jiaquan , Zhang Zerui , Xie Xiaoyun , Chu Pengpeng 
Published: May 2020

Abstract
This study evaluated the effects of temporary external voltage on the performance of two-chambered microbial fuel cells (MFC) that use nitrate wastewater as a substrate. Results indicate that the external voltage affected the performance of the MFC during their operation, and this effect remained even after the voltage was removed. The degradation efficiency of the chemical oxygen demand increased in the MFC under external voltages of 0.5, 0.8, and 1.1 V, and the optimal applied voltage was 1.1 V. Compared with the control group without external voltages, the MFC under a voltage of 1.1 V achieved higher current densities and efficiency of nitrate removal during their operation. The MFC with an applied voltage of 1.1 V also achieved the highest maximum power density of 2,035.08 mW/m3. The applied voltages of 0.5 and 0.8 V exerted a positive effect on the performance of the MFC. High-throughput sequencing was used to explore the anode and cathode biofilms. Results showed that the influence was highly associated with microbial community in bio-anode. The predominant functional family changed from Methanotrichaceae during start-up to Flavobacteriaceae in a steady phase.
Analysis of eutrophication potential of municipal wastewater
Authors: Preisner M. , Neverova-Dziopak E. , Kowalewski Z. 
Published: May 2020

Abstract
One of the main factors of the increased eutrophication level of surface waters is the high anthropogenic loads of biogenic substances discharged into water bodies. Municipal wastewaters, containing large amounts of nitrogen and phosphorus play one of the key roles in the acceleration of eutrophication intensity. The main direction in the prevention of eutrophication caused by wastewater discharge has become the reduction of nutrient loads introduced to wastewater receivers in accordance with strict legal requirements achievable only in advanced technologies. The treated wastewater quality standards are actually developed for total nitrogen and total phosphorus content, disregarding the fact that eutrophication potential of treated wastewater is determined by the content of non-organic nutrient forms directly bioavailable for water vegetation. That is why the currently used energy-consuming and expensive technologies do not always guarantee effective protection against eutrophication and its consequences. The goal of the study was to analyze the most widely used wastewater treatment technologies for enhanced biological nutrients removal in treated wastewater eutrophication potential. For this purpose, an analysis of the operation of 18 wastewater treatment plants based on different technologies in Finland, Canada, Poland, Russia and the United States was realized. The analysis concluded that the eutrophication potential of treated wastewater to a large extent is conditioned by the applied technology. The results of the research concluded that the eutrophication potential can serve an important criterion for decision-making regarding the proper selection of wastewater treatment technologies aimed at eutrophication mitigation.
A technológiai fejlődés lehetséges munkahelyteremtő-, és romboló hatásai
Authors: Boros József 
Published: May 2020

A történelem során a technológiai változások gyakran a munkavállalói szorongások alapját szolgálták. 1811 és 1816 között Angliában a munkavállalók egy csoportja – akik „Luddistáknak” nevezték magukat – tönkretették azokat a gépeket, amelyekről azt gondolták, hogy veszélyeztetik a munkahelyüket. A 19. századi gondolkodók és közgazdászok, mint Karl Marx és David Ricardo azt jósolták, hogy a gazdaság gépesítése végső soron a munkavállalói körülményeket rontaná, megfosztva őket attól, hogy egy elfogadható szintű bérből élhessenek. Az elmúlt évszázadban John M. Keynes (az 1930-as években) és Wassily Leontief (az 1950-es években) azon félelmüket fogalmazták meg, hogy egyre több és több munkást váltanak ki gépi megoldásokkal, amely munkanélküliséghez vezet. Az utóbbi években Brynjolfsson és McAfee (2014) állította, hogy a jelenlévő technológiák csökkentik a munkaerő iránti keresletet és az emberi munkaerő egy részét tartós hátrányba sodorják. Azonban számos kompenzációs mechanizmus létezik, amelyek ellensúlyozhatják az automatizálás és általában a folyamatinnováció kezdeti munkaerő-kiszorító hatásait (Vivarelli, 2015). Először is, miközben a munkavállalókat kiváltják azokban az iparágakban, amelyekben bevezetik az új gépekbe épített technológiát, további munkavállalókra van szükség az új gépeket gyártó iparágakban. Másodszor, az automatizálás (és általánosságban a folyamatinnováció) csökkenti az átlagos költségeket. Acemoglu és Restrepo (2017) azt állapították meg, hogy ez egyrészről ár-termelékenységi („priceproductivity”) hatást eredményez (mivel a termelési költségek csökkennek, az iparág kibővítheti és növelheti munkaerő-igényét); és másrészről a termelés méretgazdaságossági hatásához vezet (az automatizálás miatti költségcsökkenés a teljes kibocsátás növekedéséhez vezet, és minden iparágban növeli a munkaerő iránti igényt). Hasonlóképpen, Vivarelli (2015) azzal érvel, hogy az alacsonyabb átlagköltségek alacsonyabb árakat eredményezhetnek (ha az ipar piaci struktúrája tökéletesen versenyképes), stimulálják a termékkeresletet, vagy extra nyereséget eredményezhetnek (ha az ipar szerkezete nem tökéletesen versenyképes). Ha ezeket az extra nyereségeket újra befektetik a cégbe, ez a beruházás új munkahelyeket teremthet. Az előadás ezen ellensúlyozó eseteket szándékozik bemutatni és valós példákkal alátámasztani a szakirodalom alapján.
A millenniumi generáció munkatapasztalatai
Authors: Liskuné Vathy Veronika , Bognár István Ádám , Kőmíves Péter Miklós , Körösparti Péter , Nagy Adrián Szilárd 
Published: May 2020

Ha nyitott szemmel járunk a mindennapok során, akkor nem kerülheti el figyelmünket a hallgatói munkavégzés jelensége. Ezen tanulmány keretein belül az volt a célunk, hogy megvizsgáljuk a Debreceni Egyetem Gazdaságtudományi Karán első évfolyamon tanulmányaikat megkezdő hallgatók munkatapasztalatainak meglétét, későbbi elhelyezkedési terveit, jövedelmi igényeit. Emellett szakirodalmi vizsgálatok keretében elemezzük a hallgatói munkavégzéssel kapcsolatos legfontosabb forrásokat.
A felsőoktatásba újonnan belépő hallgatók szemléletének változására fontos figyelmet fordítani, mivel tanulmányaik befejezése után aktív munkakeresővé válnak. A Debreceni Egyetem Gazdaságtudományi Karán a 2019/2020-as tanévben mintegy 2000 fő került felvételre, akik közül 1700 meg is kezdte a tanulmányait. A kutatásunkat ezen hallgatók bevonásával végeztük.
Az adatfelvétel a Debreceni Egyetem által szervezett gólyatáborában résztvevő hallgatók körében történt. A felmérés kiterjed többek között a kezdő fizetési igények felmérésére, az eddigi munkatapasztalatokra, a jövőbeni tervekre, ehhez kapcsolódóan a külföldi munkavállalással kapcsolatos hajlandóságra, valamint arra, hogy mely tényezőket veszik figyelembe a munkahely választás során.
Az innováció lehetséges hatása a sportágak versenyképességére
Authors: Madarász Tamás 
Published: May 2020

A sportágakhoz kapcsolódó innovációs és fejlesztő tevékenységek, illetve a folyamatok során létrejövő eszközök elősegítik a teljesítménykényszerben lévő sportági szereplőket (sportoló, edző, sportvezető), hogy szakágukban kiemelkedő eredményeket érjenek el.
A sportági eredményesség nagymértékben kihathat a sportágak társadalmi megítélésére és a gazdasági potenciáljukra, ezáltal a szakágak jövőjére is. Tanulmányomban arra vállalkozom, hogy bemutassam a versenysporthoz kapcsolódó innovációs folyamatokat, továbbá ezen újító tevékenységek során létrejövő termékeket, majd megvizsgáljam, hogy ezen kellékek hogyan és milyen mértékben befolyásolják a vizsgált sportágak gazdasági és sportszakmai eredményességét.
A stressz és a szabadidősport jelenléte a mai emberek életében
Authors: Kinczel Antónia 
Published: May 2020

A mai embereket rendkívűl sok dolog lefoglalja, mint például a munka, a család, a tanulás, ezért a szabadidejüket olyan tevékenységekkel próbálják eltölteni, amelyek elősegítik őket a relaxációban, megnyugvásban, feltöltődésben. Elmondható, hogy a mai világ emberei le vannak terhelve, sokkal stresszesebbek és idegesebbek, próbálnak a legjobban teljesíteni, hogy megfelelően éljenek. Sok embernek nehezére esik a kommunikáció, a közösségi élet. Rengetegen vannak, akik rákényszerűlnek arra, hogy otthonról is dolgozzanak, mivel a projektek és többhetes határidők nem várnak. Ezek mellett még rengeteg dolgot el kell végezni, kell foglalkozni a gyermekekkel, kell szórakozni, figyelni kell az egészségre, háztartást kell irányítani stb., így kevés idő marad arra, hogy azt végezzék az emberek amit igazából szeretnek. A kutatásomban felmértem a romániai és magyarországi lakosok szabadidős szokásait, a sportban való aktivításukat ugyanakkor az aktív rekrációs tevékenységekhez való hozzáállásukat (N=273). Az eredmények azt mutatják, hogy még mindig a passzív szabadidős tevékenységek (tv nézés, olvasás, zenehallgatás, internetezés) a legnépszerűbbek az emberek körében, bár legtöbben ülő munkát végeznek. Azok az emberek, akik szabadidejükben sportolnak, sokkal felfrissültebbnek érezték maukgat, továbbá jobb volt a hangulatuk, pozitívabban gondolkodtak. Főleg azok a sportok kerülnek előtérbe, amelyeket az emberek a szabadban, a parkokban űzhetik.
Casting Issues of Thick-Walled High Pressure Die Castings
Authors: Molnár Dániel , Kiss Ádám , Majoros Csaba 
Published: May 2020

Die casting is a manufacturing process for producing accurately dimensioned, sharply defined, smooth or textured surface metal parts. It is accomplished by injecting liquid metal at fast velocity and under high pressure into reusable steel dies. Compared to other casting processes, die casting is at the top end of both velocity and pressure. The high velocity translates into a very turbulent flow condition. The process is often described as the shortest distance between raw material and the finished product.
Pre-fill is a process technique that is the result of significantly delaying the start of fast shot beyond the “metal at the gate” position. It can be stated as a percentage of cavity fill or as a distance beyond metal at the gate.
Citizenship Ceremonies in Germany: A More Universalist Kulturnation
Authors: Jacob Maria 
Published: May 2020

After 2000, a profound liberalization of naturalization criteria in Germany was followed by culturalist setbacks: language requirements were tightened, a civics test, a pledge of allegiance, and citizenship ceremonies were introduced. Can these developments be termed a liberal assimilationist turn in what it means to be a citizen or is this a revival of the old notion of a German cultural identity as Kulturnation (cultural nation)? The qualitative study of German citizenship ceremonies presented herein, provides an in-depth analysis of the logics of “liberal assimilation” and the nexus between culture, citizenship, and national belonging. At the ceremonies, “culture” is referenced as a universally human feature that binds people together. Conversely, culture is also presented as an individual folkloristic asset that can be used for profit and for contributing to the value of diversity. This twofold conceptual specification of “culture” is then discussed as an adaptation of the originally universalist Kulturnation idea and as a modern solution to the problem of societal integration.
Full text available at: https://doi.org/10.22215/rera.v11i1.256
Re-Discovering the Importance of Citizenship Through Immigrants' Experiences: Naturalization and Political Integration in Padua, Italy
Authors: Shkopi Eriselda , Vathi Zana 
Published: May 2020

This paper focuses on processes of political integration for immigrants in the Italian context, constituting as it does an understudied topic. It does so by looking at one specific community, Albanian immigrants, who have been typically heavily stigmatized. While Albanian immigration in Italy has been a focus of previous research, no consideration so far has been given to naturalization and its influence on other political processes at the level of immigrants’ daily lives. Through the meanings which participants of this research attribute to citizenship and their acting as political agents, the paper unpacks the relations between this "status passage" (Glaser and Strauss 1971) and the political integration of immigrants. The findings show a very complex picture in which multiple factors and interactions play an important role. Legally speaking, Italian citizenship is a pre-condition for immigrants to enjoy the right to vote in elections at all levels, which participants considered a significant indicator of their political integration. Therefore, the political integration of immigrants is heavily conditioned by naturalization, which gives access to political rights, voice and representation as regulated at the state level. However, when considering the role of age and social capital in processes of political integration, there is also reason to believe that the political mobilization and participation of the youngest and most well-educated participants is not as exclusively attached to such formal recognition as a political subject.

Full text available at: https://doi.org/10.22215/rera.v11i1.258
Introduction: Integration Through Crises
Authors: D'Erman Valerie , Verdun Amy 
Published: May 2020

The introductory paper to this Special Issue discusses the idea of crisis in relation to European integration from a historical perspective in order to contextualize four different current events in the European Union (EU) in turn – euro area crisis, migration crisis, Brexit, and the rise of populist responses to EU governance. We turn to the wider scholarly concept of ‘crisis’ and apply it to large-scale events affecting the EU, in order to relate events to broader theoretical discussions about the progression of the EU. Existing literature on the topic highlights different varieties of crisis scenarios: those that undermine the basic integrity of the undertaking; those that threaten certain domains or the activities of certain groups; and those that reflect short-term, but acute dangers that may be overcome without structural damage. This introductory contribution situates each of the four above-mentioned ‘crises’ in the context of these varieties and offers suggestions for how each crisis might influence the future direction of European integration by using illustrations from each of the articles in this special issue.

Full text available at: https://doi.org/10.22215/rera.v12i1.1230
The Union of Laughter and Forgetting? The European Union and the Construction of its own History
Authors: Tesler-Mabe Hernan 
Published: May 2020

As recently as one year ago, the European Union was seemingly on a direct path toward its avowed goal of "ever closer union." In numerous publications, EU authorities asserted that they had the confidence of European peoples desirous only of further integration. In the wake of the failed referenda for a European Constitution, however, enthusiasts of European Union can no longer be certain that their enterprise will succeed. The European Union, once strong and united, seems now an entity teetering on the edge of collapse. The reasons for such a dramatic shift are, of course, wide-ranging. Yet I would suggest that a great part of the general European disillusionment with European Union has come about as a result of the actions of the Europeanists themselves. Over the last decades, European officials have exhibited a frightfully high incidence of revisionism in their literature. This practice, I argue, has caused many Europeans to question the integrity of the project of European Union. For my presentation, I intend to undertake a close study of a selection of documents published by the European Communities. In this endeavour, I will compare and contrast the messages imparted in different editions of these works and consider the semiotic significance of the textual and non-textual language appearing therein. In this manner, I hope to achieve two aims. First, I mean to add a corrective element to a literature that, guided by a teleological interpretation of integration, endows integration with”logic" to be found only in hindsight. Second, I intend to examine the many meanings that the EU has had over its history and assess how closely policy has adhered to the ideological goals of prominent Europeanists. In sum, I hope to shed light on the fundamental disconnect between advocates of Europe and the "man on the street" and help establish a dialogue which may contribute to resolving the current impasse within the European Union.
Full text available: https://doi.org/10.22215/rera.v2i4.178
Propaganda Instruments in Contemporary Campaigns: Comparison of Estonian Political Television Advertisements and Modern Television Commercials
Authors: Uudelepp Agu 
Published: May 2020

The author argues in the present article that although propaganda is considered mostly a tool of ideological communication suitable for use during wars or in totalitarian states, it is still used in contemporary democratic societies at peacetime and there are no major differences between employing instruments of propaganda in the public or the private sectors. The present analysis is based on the similarities and differences between Estonian political television advertisements and modern television commercials with an emphasis on the application of propaganda instruments. The author employed content analysis when studying the sample in which were 100 non-political and 84 political advertisements. This research shows that Estonian political television advertisements and international non-political television advertisements share some significant similarities: cognitive propaganda instruments are more widely employed than social ortechnological ones. The role oftechnological propaganda instruments is diminishing and such instruments are replaced by structural ones. A major difference is that, on average, there are more propaganda instruments per advertisement in political television advertisements than in non-political television advertisements, and technological propaganda instruments are not employed in non-political television advertisements.
Full text available: https://doi.org/10.22215/rera.v3i1.182