bluuebunny/crossref_metadata_2025_split huggingface dataset:
The safety and effectiveness of genetically corrected iPSCs derived from β-thalassaemia patients in nonmyeloablative β-thalassaemic mice
Authors: Xian Yexing , Xie Yingjun , Song Bing , Ou Zhanhui , Ouyang Shuming , Xie Yuhuan , Yang Yi , Xiong Zeyu , Li Haoxian , Sun Xiaofang 
Published: July 2020

Abstract
Background
β-Thalassaemia is a clinically common cause of hereditary haemolytic anaemia stemming from mutations in important functional regions of the β-globin gene. The rapid development of gene editing technology and induced pluripotent stem cell (iPSC)-derived haematopoietic stem cell (HSC) transplantation has provided new methods for curing this disease.

Methods
Genetically corrected β-thalassaemia (homozygous 41/42 deletion) iPSCs that were previously established in our laboratory were induced to differentiate into HSCs, which were transplanted into a mouse model of IVS2–654 β-thalassaemia (B6;129P2-Hbbtm2Unc/J mice) after administration of an appropriate nonmyeloablative conditioning regimen. We also investigated the safety of this method by detecting the incidence of tumour formation in these mice after transplantation.

Results
The combination of 25 mg/kg busulfan and 50 mg/(kg day) cyclophosphamide is an ideal nonmyeloablative protocol before transplantation. Genetically corrected β-thalassaemic HSCs survived and differentiated in nonmyeloablated thalassaemia mice. No tumour formation was observed in the mice for 10 weeks after transplantation.

Conclusion
Our study provides evidence that the transplantation of genetically corrected, patient-specific iPSCs could be used to cure genetic diseases, such as β-thalassaemia major.
Advanced organ support (ADVOS) in the critically ill: first clinical experience in patients with multiple organ failure
Authors: Fuhrmann Valentin , Weber Theresa , Roedl Kevin , Motaabbed Jasmin , Tariparast Adel , Jarczak Dominik , de Garibay Aritz Perez Ruiz , Kluwe Johannes , Boenisch Olaf , Herkner Harald , Kellum John A. , Kluge Stefan 
Published: July 2020

Abstract
Background
Prevalence of multiple organ failure (MOF) in critically ill patients is increasing and associated mortality remains high. Extracorporeal organ support is a cornerstone in the management of MOF. We report data of an advanced hemodialysis system based on albumin dialysis (ADVOS multi device) that can regulate acid–base balance in addition to the established properties of renal replacement therapy and albumin dialysis systems in critically ill patients with MOF.

Methods
34 critically ill patients with MOF received 102 ADVOS treatment sessions in the Department of Intensive Care Medicine of the University Medical Center Hamburg-Eppendorf. Markers of metabolic detoxification and acid–base regulation were collected and blood gas analyses were performed. A subgroup analyses were performed in patients with severe acidemia (pH < 7.2).

Results
Median number of treatment sessions was 2 (range 1–9) per patient. Median duration of treatment was 17.5 (IQR 11–23) hours per session. Treatment with the ADVOS multi-albumin dialysis device caused a significant decrease in bilirubin levels, serum creatinine, BUN and ammonia levels. The relative elimination rate of bilirubin was concentration dependent. Furthermore, a significant improvement in blood pH, HCO3− and PaCO2, was achieved during ADVOS treatment including six patients that suffered from severe metabolic acidosis refractory to continuous renal replacement therapy. Delta pH, HCO3− and PaCO2 were significantly affected by the ADVOS blood flow rate and pH settings. This improvement in the clinical course during ADVOS treatments allowed a reduction in norepinephrine during ADVOS therapy. Treatments were well tolerated. Mortality rates were 50% and 62% for 28 and 90 days, respectively.

Conclusions
In this case series in patients with MOF, ADVOS was able to eliminate water-soluble and albumin-bound substances. Furthermore, the device corrected severe metabolic and respiratory acid–base disequilibrium. No major adverse events associated with the ADVOS treatments were observed.
Research on robot boxing movement simulation based on kinect sensor
Authors: Li Peicong 
Published: July 2020

AbstractIn the process of continuous development, robot technology has been widely used in aerospace, medical, education, and service industries, and the relationship between robots and people is getting closer and closer. The improvement of robot intelligence is a process of continuous learning of external knowledge. Since the introduction of human-computer interaction technology, it has provided more and more technical support for human research robots. This paper firstly takes Tai Chi as an example, analyzes and deconstructs its standard Tai Chi putter movements, and obtains the corresponding movement parameter information. Then, the human body depth image and bone structure are obtained through the kinect sensor, and the human joint position data is recorded, and then the coordinate data is sent to the brain robot, thereby realizing the imitation learning of the robot’s push movements in Tai Chi. The experimental results show that the proposed method can simulate the practice process of inversion of Tai Chi, can well analyze the shortcomings in the practice process, and can then promote the development of the sport.
Integral inequalities for a fractional operator of a function with respect to another function with nonsingular kernel
Authors: Mohammed Pshtiwan Othman , Abdeljawad Thabet 
Published: July 2020

AbstractAt first, we construct a connection between the Atangana–Baleanu and the Riemann–Liouville fractional integrals of a function with respect to a monotone function with nonsingular kernel. By examining this relationship and the iterated form of Prabhakar fractional model, we are able to find some new Hermite–Hadamard inequalities and related results on integral inequalities for the two models of fractional calculus which are defined using monotone functions with nonsingular kernels.
Box-scheme as alternative food network—the economic integration between consumers and producers
Authors: de Souza Ronaldo Tavares 
Published: July 2020

AbstractAs a growing option for small farmers, alternative food networks (AFN) have attracted the attention of researchers around the world. Nevertheless, not much focus is given to box-schemes as a format itself. The nomenclature is often associated with community-supported agriculture (CSA) and solidarity purchase groups (SPG), but many companies have reduced geographic and informational distances without demanding such a narrow relationship between collaborators. The aim of this article is to analyze the relationship between companies and their customers. A theoretical framework was built based on thoughts about economic integration and the relationship in the retail environment to examine the possibility of reciprocity and redistribution. The fieldwork was conducted in São Paulo, Brazil. Technology is found to be fundamental to the rise of such companies, but the Internet is not the only key, the telephone is also important for a warmer and closer relationship. The interviews revealed different signals of reciprocity supporting a trusting relationship while the occasional buyers demonstrate more opportunistic behaviors. Of the cases studied, one successful company demonstrated signs of reciprocity between companies and customers, as well as customer integration based on redistribution characteristics. This confirmed the possibility of the box-scheme contributing to rural sustainability, related to customers who have built a broader perspective, going beyond price and quality. The article concludes defending that this format of commerce should be further studied to confirm its capacity to support rural development and continue to deepen the understanding of the conditions that can make it successful.
Development and validation of an ELISA for a biomarker of thyroid dysfunction, thyroid peroxidase autoantibodies (TPO-Ab), in dried blood spots
Authors: Eick Geeta N. , Cepon-Robins Tara J. , Devlin Maureen J. , Kowal Paul , Sugiyama Larry S. , Snodgrass J. Josh 
Published: July 2020

Abstract
Background
The prevalence of allergic and autoimmune conditions has been steadily increasing in wealthy nations over the past century. One hypothesis put forward to explain this is the Old Friends Hypothesis, which posits that increased hygiene, urbanization, and lifestyle changes have reduced our exposure to parasites and microbes that we co-evolved with, resulting in immune dysregulation. However, research in traditionally living populations, who are exposed to greater parasite and pathogen loads such as those encountered during our evolution, is limited, in part due to a lack of minimally invasive, field-friendly biomarkers of autoimmune disorders. We therefore developed an ELISA to assess positivity for thyroid peroxidase autoantibody (TPO-Ab), an indicator of autoimmune thyroid disease, based on dried blood spot (DBS) samples.

Results
We used the Accubind anti-thyroid peroxidase test system to screen our validation samples comprising matched fingerprick DBS, venous DBS, and plasma samples from 182 adults. After confirming that we had TPO-Ab-positive individuals in our validation sample (n = 12), we developed an indirect ELISA to measure TPO-Ab levels from one 3-mm DBS punch. The sensitivity and specificity of our assay for DBS samples ranged from 91.7–100% and 98.2–98.8%, respectively, using a cut-off value of ≥ 26 IU/mL. Intra-assay reliability for duplicate quality control DBS punches was 5.2%, while inter-assay reliability ranged from 11.5–24.4% for high, medium, and low DBS controls. Dilutional linearity ranged from 80 to 120%, and spike and recovery experiments indicated that the DBS matrix does not interfere with the detection of TPO-Ab. TPO-Ab levels remained stable in DBS samples stored at − 28 °C or − 80 °C, but decreased over time in DBS samples kept at 22 °C or at 37 °C.

Conclusions
We developed an in-house, kit-independent indirect ELISA assay to determine individuals’ TPO-Ab positivity based on dried blood spots, representing a cost-effective method with potential applications in a range of research settings.
Influence of particle aggregation on the tephra dispersal and sedimentation from the October 8, 2016, eruption of Aso volcano
Authors: Tsuji Tomohiro , Nishizaka Naoki , Ohnishi Kozo 
Published: July 2020

AbstractThe phreatomagmatic eruption at Aso volcano on October 8, 2016, released PDC and fallout up to 320 km from the vent, providing an opportunity to study the dispersal and sedimentation of tephra bearing wet and dry aggregates. We investigated fresh air-fall tephra and conducted both dry- and wet-sieving to assess the size distribution of both aggregate-bearing deposits on the ground and particles in the plume prior to aggregation. The tephra was asymmetrically distributed in the ENE direction, with a relatively wide distribution and small maximum grain sizes north of the depositional axis, reflecting different wind directions at each altitude. The secondary accumulation maxima at 4–8 km NE from the vent was characterized by the segregation of lapilli followed by accretionary pellets that were rapidly created by aggregation in the wet plume, thereby enhancing the removal of airborne ash. Coalesced aggregates possibly arose from salt precipitation processes. For dry aggregates fallen in medial-to-distal regions, observed deposition duration and fall velocity calculations concluded that fine ash was deposited not as individual particles but as aggregates. Most grain-size distributions along the dispersal axis based on the two sieving methods used were multimodal, with the coarser mode fining according to distance from the vent and the finer mode stabilizing at around 4ϕ; this is interpreted as resulting from the mixture of grain-size distributions with different origins, i.e., the normal settling of individual clasts and particle aggregation. The wet-sieved total grain-size distribution included 20.4 wt% fine ash compared with only 2.4 wt% fine ash in the dry-sieved distribution, suggesting that aggregated particles accounted for 18 wt% of the deposit. Total grain-size distributions based on wet-sieving showed extremely poor sorting that could be explained by the following causes: the release of coarse-grained ejecta originating from the host rock of the crater, fine particles due to fragmentation from a phreatomagmatic eruption, the supply of water and salt from the crater to the plume, and aggregation enhancement of the fine ash on the ground, reflecting the effect of the crater lake as external water source.
Reproductive performance of laying snails (Archachatina marginata) fed on roughages and different concentrate mixes
Authors: Jimoh Olatunji Abubakar , Akinola Micheal Olawale 
Published: July 2020

Abstract
Background
This study aims to investigate the reproduction output of Archachatina marginata fed on roughage and concentrate feed with leaf meal-inclusive diets. One hundred fifty points of lay snails weighing between 150 and 170 g were randomly allotted to 5 treatment diets with three replicates, ten snails per replicate, designated as treatment 2 (GNCD) which has groundnut cake, treatment 3 (GLLM) has Gliricidia sepium, treatment 4 (MOLM) has Moringa oleifera, and treatment 5 (LLLM) has Leucaena leucocephala leaf meals as protein source in snail diet and a control (treatment 1) (UPWP) fed on roughage (unripe pawpaw and watermelon peel) in a 12-week feed trial. Feed intake, weight gain, egg lay, egg fertility, hatchability, and gonadosomatic index were measured.

Results
The result revealed snails fed with UPWP had the least final weight and weight gain which was accompanied by the highest feed intake across the treatments. Average egg laid, hatchability, fertility, embryo mortality, and average juvenile produced by snails on roughage-based diet were significantly the least across the treatments. High gonadosomatic index of snails on leaf meal-based diet accompanied with better reproductive output in laying snails was recorded.

Conclusion
Snails fed with Leucaena leucocephala-inclusive diet had better egg production, fertility, hatchability, and juvenile snails accompanied with gonadosomatic index across the treatments. Concentrate ration is essential for optimal reproductive output in snails.
Pregnancy After Breast Cancer in Patients With Germline BRCA Mutations
Authors: Lambertini Matteo , Ameye Lieveke , Hamy Anne-Sophie , Zingarello Anna , Poorvu Philip D. , Carrasco Estela , Grinshpun Albert , Han Sileny , Rousset-Jablonski Christine , Ferrari Alberta , Paluch-Shimon Shani , Cortesi Laura , Senechal Claire , Miolo Gianmaria , Pogoda Katarzyna , Pérez-Fidalgo Jose Alejandro , De Marchis Laura , Ponzone Riccardo , Livraghi Luca , Estevez-Diz Maria Del Pilar , Villarreal-Garza Cynthia , Dieci Maria Vittoria , Clatot Florian , Berlière Martine , Graffeo Rossella , Teixeira Luis , Córdoba Octavi , Sonnenblick Amir , Luna Pais Helena , Ignatiadis Michail , Paesmans Marianne , Partridge Ann H. , Caron Olivier , Saule Claire , Del Mastro Lucia , Peccatori Fedro A. , Azim Hatem A. 
Published: July 2020

PURPOSE Young women with germline BRCA mutations have unique reproductive challenges. Pregnancy after breast cancer does not increase the risk of recurrence; however, very limited data are available in patients with BRCA mutations. This study investigated the impact of pregnancy on breast cancer outcomes in patients with germline BRCA mutations. PATIENTS AND METHODS This is an international, multicenter, hospital-based, retrospective cohort study. Eligible patients were diagnosed between January 2000 and December 2012 with invasive early breast cancer at age ≤ 40 years and harbored deleterious germline BRCA mutations. Primary end points were pregnancy rate, and disease-free survival (DFS) between patients with and without a pregnancy after breast cancer. Pregnancy outcomes and overall survival (OS) were secondary end points. Survival analyses were adjusted for guarantee-time bias controlling for known prognostic factors. RESULTS Of 1,252 patients with germline BRCA mutations ( BRCA1, 811 patients; BRCA2, 430 patients; BRCA1/2, 11 patients) included, 195 had at least 1 pregnancy after breast cancer (pregnancy rate at 10 years, 19%; 95% CI, 17% to 22%). Induced abortions and miscarriages occurred in 16 (8.2%) and 20 (10.3%) patients, respectively. Among the 150 patients who gave birth (76.9%; 170 babies), pregnancy complications and congenital anomalies occurred in 13 (11.6%) and 2 (1.8%) cases, respectively. Median follow-up from breast cancer diagnosis was 8.3 years. No differences in DFS (adjusted hazard ratio [HR], 0.87; 95% CI, 0.61 to 1.23; P = .41) or OS (adjusted HR, 0.88; 95% CI, 0.50 to 1.56; P = .66) were observed between the pregnancy and nonpregnancy cohorts. CONCLUSION Pregnancy after breast cancer in patients with germline BRCA mutations is safe without apparent worsening of maternal prognosis and is associated with favorable fetal outcomes. These results provide reassurance to patients with BRCA-mutated breast cancer interested in future fertility.
Playing against Type: The Moral Merchant on the Early Qing Stage
Authors: Fox Ariel 
Published: July 2020

Abstract
This article explores the changing depiction of the merchant and the mercantile in the early Qing. A figure of much anxiety and mistrust in the late imperial imagination, the traveling merchant moves things out of their proper place—through both his movement of goods across space and his own mobility across social strata. In the early Qing play Shiwu guan 十五貫 (Fifteen Strings of Cash), however, the merchant's facilitation of the circulation of money and man does not trouble the social whole as much as constitute it. The merchant-hero breaks through narrative and economic impasses, directing capital away from the dead ends of hoarding and incest and toward the creation of productive marriages. In recuperating the merchant as a moral subject and his circulation of money as a moral act, Shiwu guan offers new possibilities for the construction of selfhood both onstage and off.
The Ancient Chinese Arts of the Ear: Etymology, Meteorology, Musicology
Authors: Xiaodun Wang , Schoenberger Casey 
Published: July 2020

Abstract
This article draws on historical linguistic evidence, archeological finds, and written accounts of ancient practices to argue that, in the pre-Qin and Han periods of Chinese history, an important stratum of knowledge related to earthly energies, vibrations, pitch, tonality, music, memory, and recitation existed in conceptual parallel to systems of visual knowledge of heavenly bodies, light, color, and the written record. Masters of the former set of skills were frequently blind and entrusted with a distinct set of ritual and advisory functions, including ushering in the seasons, pronouncing on elements of the calendar, predicting military fortunes, and performing official policy admonishments. Of particular importance to this group of experts was the concept of “winds” or “airs” (fēng) and a closely related verb for “sing,” “chant,” or “remonstrate” (fĕng). The etymological relationship of these words, along with words for listening, smell, sounds, and fragrance, led to a conceptual blending whereby the “energy” (qi) of wise words and “fragrant” virtue could carry on “winds” of oral transmission to correct public morality and governance. This led to an etiological hierarchy, in some ways inverted by current standards, in which the purpose of studying pitch and tonality was not, first and foremost, analysis of music qua art but, rather, the encoding, transmission, and influence of natural energies and social harmony.
Tao Yuanming in Recently Unearthed Epitaphs from the Sui and Tang
Authors: Kexian Hu , Zhang Yuan 
Published: July 2020

Abstract
A recently discovered collection of epitaphs (muzhi 墓誌) reveals copious references to Tao Yuanming (Tao Qian 陶潛, 365–427), a writer of pervasive influence on Chinese culture. In recent decades, both English and Chinese scholarship has focused on Tao's literary and historical reception, with little attention paid to his representation in epitaph writing. This article, through an examination of these newly unearthed documents, presents forty-seven epitaph fragments with direct mention of Tao's name. Most were written in the Tang dynasty, when Tao was ardently appreciated as a poet, and his literary vocabulary was widely borrowed and imitated. However, a close reading of epitaphs illustrates that Tao's image as a moral exemplar was perhaps even more prominent than his role as esteemed poet. He was invoked to suggest the comparable personal traits of the tomb owner (muzhu 墓主), his name frequently juxtaposed with various historical figures renowned for their virtue. His name is also used as an adjective to modify carefully selected images to further characterize him as a moral exemplar. In Tang epitaphs, moral concerns together with philosophical contemplation on the motives of reclusion play a significant role, laying the foundation for the complexity of Tao's image in the Song period. Current research seeks to increase our understanding of the process behind the construction of Tao as a cultural icon.
Mitochondrial dynamics during spermatogenesis
Authors: Varuzhanyan Grigor , Chan David C. 
Published: July 2020

ABSTRACT
Mitochondrial fusion and fission (mitochondrial dynamics) are homeostatic processes that safeguard normal cellular function. This relationship is especially strong in tissues with constitutively high energy demands, such as brain, heart and skeletal muscle. Less is known about the role of mitochondrial dynamics in developmental systems that involve changes in metabolic function. One such system is spermatogenesis. The first mitochondrial dynamics gene, Fuzzy onions (Fzo), was discovered in 1997 to mediate mitochondrial fusion during Drosophila spermatogenesis. In mammals, however, the role of mitochondrial fusion during spermatogenesis remained unknown for nearly two decades after discovery of Fzo. Mammalian spermatogenesis is one of the most complex and lengthy differentiation processes in biology, transforming spermatogonial stem cells into highly specialized sperm cells over a 5-week period. This elaborate differentiation process requires several developmentally regulated mitochondrial and metabolic transitions, making it an attractive model system for studying mitochondrial dynamics in vivo. We review the emerging role of mitochondrial biology, and especially its dynamics, during the development of the male germ line.
High Sensitive Electrochemical Nitrite Sensor Using Fe2O3/MoS2 Nanocomposites Synthesized by Facile Method
Authors: Arunbalaji Subramanian , Ismail Mohamed Abubakkar Mohamed , Arivanandhan Mukannan , Alsalme Ali , Alghamdi Abdulaziz , Jayavel Ramasamy 
Published: July 2020

Abstract
An amperometric nitrite sensor is fabricated using Fe2O3/MoS2 (FM) nanocomposites synthesized by solution mixing process. Structural properties of the nanocomposite are studied by XRD analysis. Morphological studies by HRTEM analysis reveal the formation of nanocomposite comprising Fe2O3 nanoplates on MoS2 nanosheets. The composition of the nanocomposite has been studied by EDX analysis. The fabricated amperometric sensor can be used for a wide range of sensing from 10 µM–10 mM with a lower limit of detection (LOD) of 0.2 µM. The high sensitivity (310 µA mM−1 cm−2), high stability and low interference of FM nanocomposites demonstrate that the prepared composite is a promising material for nitrite sensing in drinking water.