bluuebunny/crossref_metadata_2025_split huggingface dataset:
δ subunit‐containing GABAA IPSCs are driven by both synaptic and diffusional GABA in mouse dentate granule neurons
Authors: Sun Min‐Yu , Ziolkowski Luke , Mennerick Steven 
Published: January 2020

Key points
Current views suggest γ2 subunit‐containing GABAA receptors mediate phasic IPSCs while extrasynaptic δ subunits mediate diffusional IPSCs and tonic current.
We have re‐examined the roles of the two receptor populations using mice with picrotoxin resistance engineered into receptors containing the δ subunit.
Using pharmacological separation, we find that in general δ and γ IPSCs are modulated in parallel by manipulations of transmitter output and diffusion, with evidence favouring modestly more diffusional contribution to δ IPSCs.
Our findings also reveal that spontaneous δ IPSCs are mainly driven by channel deactivation, rather than by diffusion of GABA.
Understanding the functional contributions of the two receptor classes may help us understand the actions of drug therapies with selective effects on one population over the other.
AbstractGABAA receptors mediate transmission throughout the central nervous system and typically contain a δ subunit (δ receptors) or a γ2 subunit (γ2 receptors). δ IPSCs decay slower than γ2 IPSCs, but the reasons are unclear. Transmitter diffusion, rebinding, or slow deactivation kinetics of channels are candidates. We used gene editing to confer picrotoxin resistance on δ receptors in mice, then pharmacologically isolated δ receptors in mouse dentate granule cells to explore IPSCs. γ2 and δ components of IPSCs were modulated similarly by presynaptic manipulations and manipulations of transmitter lifetime, suggesting that GABA release recruits δ receptors proportionally to γ2 receptors. δ IPSCs showed more sensitivity to altered transmitter release and to a rapidly dissociating antagonist, suggesting an additional spillover contribution. Reducing GABA diffusion with 5% dextran increased the peak amplitude and decreased the decay of evoked δ IPSCs but had no effect on δ or dual‐component (mainly γ2‐driven) spontaneous IPSCs, suggesting that GABA actions can be local for both receptor types. Rapid application of varied [GABA] onto nucleated patches from dentate granule cells demonstrated a deactivation rate of δ receptors similar to that of δ spontaneous IPSCs, consistent with the idea that deactivation and local GABA actions drive δ spontaneous IPSCs. Overall, our results indicate that δ IPSCs are activated by both synaptic and diffusional GABA. Our results are consistent with a functional relationship between δ and γ2 GABAA receptors akin to that of slow NMDA and fast AMPA EPSCs at glutamate synapses.
Investigation on Tomographic-Based Nondestructive Characterization of Short Glass Fiber-Reinforced Composites as Obtained From Micro Injection Molding
Authors: Rathore Jitendra Singh , Konopczyński Tomasz , Hesser Jürgen , Lucchetta Giovanni , Carmignato Simone 
Published: January 2020

Abstract
Quantitative assessment of fiber characteristics in composite parts is of great significance in order to correlate them with the fiber-induced mechanical properties. X-ray computed tomography (CT) is being successfully used as a three-dimensional nondestructive measuring technique for the analysis of fiber characteristics (mainly the fiber orientation and fiber volume content) in fiber-reinforced composite materials. However, the accuracy of such analyses depends on various factors (e.g., scanning parameters, resolution), which is the motivation for this study. The current work investigates the effect of CT scanning parameters and spatial resolution on the obtained fiber orientation and fiber volume content. First a simulation study is carried out using a computationally generated fiber composite model followed by a validation using a thin-wall injection-molded part. The findings showed that the effect of CT settings is not significant on the measurements, but the resolution affects the estimated fiber volume content adversely. A preliminary error calculation method is proposed for correcting the overestimation in the fiber volume content.
Measuring the Performance Impact of Using the Microsoft HoloLens 1 to Provide Guided Assembly Work Instructions
Authors: Hoover Melynda , Miller Jack , Gilbert Stephen , Winer Eliot 
Published: January 2020

Abstract
The human performance benefits of four types of guided assembly instructions, including the Microsoft HoloLens 1, were analyzed in the context of a realistic assembly task. Several studies have confirmed the benefits of using augmented reality (AR) work instructions over traditional digital or paper instructions, but few have compared the effects of different AR hardware, including head-mounted displays, for complex assembly tasks. Participants completed a mock wing assembly task using the Microsoft HoloLens 1, and completion time, error count, and net promoter score (NPS) were recorded. These data were compared to data from previous research which employed desktop model-based instructions (MBIs), tablet MBI, and tablet AR instructions for the same task. The use of HoloLens AR instructions led to time saving of 16% over the tablet AR instructions. HoloLens users also had lower error rates than non-AR users. Despite the performance benefits of the HoloLens AR instructions, this condition had a lower NPS than the tablet AR group. The qualitative data showed that some users thought the HoloLens device was uncomfortable and that the tracking was not always exact. Although the users favored the tablet AR condition, the HoloLens condition had significantly faster assembly times. The authors recommend using the HoloLens for complex guided assembly instructions with minor changes, such as allowing the user to toggle the AR instructions on and off at will. The results of this paper can help manufacturing stakeholders understand the benefits of different AR technologies for manual assembly tasks.
Elevated-Temperature Wear Study of HVOF spray Cr3C2–NiCr-Coated Die Steels
Authors: Chhabra Pankaj , Kaur Manpreet 
Published: January 2020

Abstract
Wear and surface damage of tools (die materials) in the hot metal forming industry is a critical problem observed and is producing an adverse effect on the process economy. The problem occurs when tool and workpiece interact at higher temperatures. However, the research related to the wear and friction of dies is still lacking. In the present investigation, tribological (wear and friction) studies were conducted with an aim to explore the potential of Cr3C2–NiCr surface coating. The high-velocity oxy-fuel (HVOF) spray technique was used to formulate the coatings. The microhardness, surface roughness, bond strength, and porosity of the coated specimens were found and analyzed. Subsequently, elevated-temperature wear and friction study of the uncoated and coated specimens were done in the laboratory. The study was conducted at 25 N and 50 N loads. The coated specimens showed an increase in wear resistance at all test parameters. The lowest value of coefficient of friction (COF) and the specific wear-rate for the coated specimens were observed at 400 °C. Wear mechanisms were studied by the scanning electron microscopy (SEM) technique. The wear mechanisms were observed to be adhesive in nature at room temperatures and the combination of abrasive/oxidative/adhesive in nature at higher temperatures for the Cr3C2–NiCr-coated specimens.
Contact Model for Incompressible Neo-Hookean Materials Under Finite Spherical Indentation
Authors: Guo Zaoyang , Lyu Qihui , Jiang Li , Chen Yang , Dong Leiting , Zhang Han 
Published: January 2020

Abstract
In this paper, a contact model is proposed to predict the contact response of an incompressible neo-Hookean half-space under finite spherical indentations. The axisymmetric finite element (FE) model is created to simulate the contact behaviors. Inspired by the numerical results, the radius of the contact circle is derived. The contact force is then obtained by modifying the radius of the contact circle of the Hertz model. The format of the distribution of the contact pressure is also developed according to the Hertz model. A parameter, determined by fitting the numerical results, is introduced to characterize the effect of the indentation depth on the shape of the distribution function of the contact pressure. The newly proposed contact model is numerically validated to predict well the contact behaviors, including the contact force, the radius of the contact circle, and the distribution of the contact pressure, for the incompressible neo-Hookean half-space under spherical indentation up to the indenter radius. However, the Hertz model is verified to offer acceptable predictions of the contact behaviors for the incompressible neo-Hookean materials within the indentation depth of 0.1 times of the indenter radius.
Modeling Backlash-Like Hysteresis of Tendon Sheath Mechanism-Pair
Authors: Hong Junho , Hong Dahie , Kim Byung Gon 
Published: January 2020

Abstract
Tendon sheath mechanism (TSM) is an effective power transmission system to access remote locations through tortuous channels, and it is widely used in endoscopic surgical robots. However, owing to deformation of TSM during power transmission, the input–output hysteresis is expressed in the form of backlash-like hysteresis. This paper is premised on the idea that sheath deformation can occur if the sheath is fixed only at certain points rather than being fully fixed at all points. Based on our hypothesis, a new TSM-pair backlash-like hysteresis model was derived, which considers the deformation property of the sheath. Experimental setups were designed to validate the new model, and its mechanical parameters were identified. The experimental results revealed that the sheath significantly deforms and that such deformation produces backlash-like hysteresis together with the tendon deformation. This model can be used to provide accurate prediction and control of TSM.
Glypican-3–Specific CAR T Cells Coexpressing IL15 and IL21 Have Superior Expansion and Antitumor Activity against Hepatocellular Carcinoma
Authors: Batra Sai Arun , Rathi Purva , Guo Linjie , Courtney Amy N. , Fleurence Julien , Balzeau Julien , Shaik Rahamthulla S. , Nguyen Thao P. , Wu Meng-Fen , Bulsara Shaun , Mamonkin Maksim , Metelitsa Leonid S. , Heczey Andras 
Published: January 2020

Abstract
Hepatocellular carcinoma (HCC) is the fourth most common cause of cancer-related death in the world, and curative systemic therapies are lacking. Chimeric antigen receptor (CAR)–expressing T cells induce robust antitumor responses in patients with hematologic malignancies but have limited efficacy in patients with solid tumors, including HCC. IL15 and IL21 promote T-cell expansion, survival, and function and can improve the antitumor properties of T cells. We explored whether transgenic expression of IL15 and/or IL21 enhanced glypican-3-CAR (GPC3-CAR) T cells' antitumor properties against HCC. We previously optimized the costimulation in GPC3-CARs and selected a second-generation GPC3-CAR incorporating a 4-1BB costimulatory endodomain (GBBz) for development. Here, we generated constructs encoding IL15, IL21, or both with GBBz (15.GBBz, 21.GBBz, and 21.15.GBBz, respectively) and examined the ability of transduced T cells to kill, produce effector cytokines, and expand in an antigen-dependent manner. We performed gene-expression and phenotypic analyses of GPC3-CAR T cells and CRISPR-Cas9 knockout of the TCF7 gene. Finally, we measured GPC3-CAR T-cell antitumor activity in murine xenograft models of GPC3+ tumors. The increased proliferation of 21.15.GBBz T cells was at least in part dependent on the upregulation and maintenance of TCF-1 (encoded by TCF7) and associated with a higher percentage of stem cell memory and central memory populations after manufacturing. T cells expressing 21.15.GBBz had superior in vitro and in vivo expansion and persistence, and the most robust antitumor activity in vivo. These results provided preclinical evidence to support the clinical evaluation of 21.15.GPC3-CAR T cells in patients with HCC.