bluuebunny/crossref_metadata_2025_split huggingface dataset:
PROtective Ventilation with a low versus high Inspiratory Oxygen fraction (PROVIO) and its effects on postoperative pulmonary complications: protocol for a randomized controlled trial
Authors: Li Xue-Fei , Jiang Dan , Jiang Yu-Lian , Yu Hong , Jiang Jia-Li , He Lei-Lei , Yang Xiao-Yun , Yu Hai 
Published: November 2019

AbstractBackgroundPostoperative pulmonary complications (PPCs) are the most common perioperative complications following surgical site infection (SSI). They prolong the hospital stay and increase health care costs. A lung-protective ventilation strategy is considered better practice in abdominal surgery to prevent PPCs. However, the role of the inspiratory oxygen fraction (FiO2) in the strategy remains disputed. Previous trials have focused on reducing SSI by increasing the inhaled oxygen concentration but higher FiO2(80%) was found to be associated with a greater incidence of atelectasis and mortality in recent research. The trial aims at evaluating the effect of different FiO2added to the lung-protective ventilation strategy on the incidence of PPCs during general anesthesia for abdominal surgery.Methods and designPROtective Ventilation with a low versus high Inspiratory Oxygen fraction trial (PROVIO) is a single-center, prospective, randomized controlled trial planning to recruit 252 patients undergoing abdominal surgery lasting for at least 2 h. The patients will be randomly assigned to (1) a low-FiO2(30% FiO2) group and (2) a high-FiO2(80% FiO2) group in the lung-protective ventilation strategy. The primary outcome of the study is the occurrence of PPCs within the postoperative 7 days. Secondary outcomes include the severity grade of PPCs, the occurrence of postoperative extrapulmonary complications and all-cause mortality within the postoperative 7 and 30 days.DiscussionThe PROVIO trial assesses the effect of low versus high FiO2added to a lung-protective ventilation strategy on PPCs for abdominal surgery patients and the results should provide practical approaches to intraoperative oxygen management.Trial registrationwww.ChiCTR.org.cn, identifier:ChiCTR18 00014901. Registered on 13 February 2018.
Renal epithelial cells retain primary cilia during human acute renal allograft rejection injury
Authors: Verghese Elizabeth , Martelotto Luciano G. , Cain Jason E. , Williams Timothy M. , Wise Andrea F. , Hill Prudence A. , Langham Robyn G. , Watkins D. Neil , Ricardo Sharon D. , Deane James A. 
Published: November 2019

Abstract

Objectives
Primary cilia are sensory organelles which co-ordinate several developmental/repair pathways including hedgehog signalling. Studies of human renal allografts suffering acute tubular necrosis have shown that length of primary cilia borne by epithelial cells doubles throughout the nephron and collecting duct, and then normalises as renal function returns. Conversely the loss of primary cilia has been reported in chronic allograft rejection and linked to defective hedgehog signalling. We investigated the fate of primary cilia in renal allografts suffering acute rejection.

Results
Here we observed that in renal allografts undergoing acute rejection, primary cilia were retained, with their length increasing 1 week after transplantation and remaining elevated. We used a mouse model of acute renal injury to demonstrate that elongated renal primary cilia in the injured renal tubule show evidence of smoothened accumulation, a biomarker for activation of hedgehog signalling. We conclude that primary cilium-mediated activation of hedgehog signalling is still possible during the acute phase of renal allograft rejection.
Analysis of the clinical significance of DNA methylation in gastric cancer based on a genome-wide high-resolution array
Authors: Fang Wen-Liang , Chen Ming-Huang , Huang Kuo-Hung , Chang Shih-Ching , Lin Chien-Hsing , Chao Yee , Lo Su-Shun , Li Anna Fen-Yau , Wu Chew-Wun , Shyr Yi-Ming 
Published: November 2019

Abstract

Background
Aberrant DNA methylation is involved in gastric carcinogenesis and may serve as a useful biomarker in the diagnosis and detection of gastric cancer (GC) recurrence.

Results
A total of 157 patients who received surgery for GC were enrolled in the present study. A genome-wide methylation analysis was performed in tumor and adjacent normal tissues for the discovery set of 16 GC patients; the top three hypermethylated CpG sites of DNA promoters were selected for validation in tissue and plasma samples for the validation set of 141 GC patients. The frequencies of the top three hypermethylated genes in available patient tissues (n = 141) and plasma samples (n = 106) were 41.8% and 38.7%, respectively, for ADAM19; 40.4% and 42.5%, respectively, for FLI1; and 56.7% and 50.9%, respectively, for MSC. In both tissue and plasma samples, FLI1 hypermethylation was associated with more advanced GC and liver and distant lymphatic metastasis, and ADAM19 hypermethylation was associated with more stage IV GC. In plasma samples, MSC hypermethylation was more common in non-superficial type GC than samples without MSC hypermethylation. In both tissue and plasma samples, patients with methylation of all the three genes had significantly more liver metastases, distant lymphatic metastases, and paraaortic lymph node metastases than patients with two or fewer hypermethylated genes. The survival analysis showed that only for stage III GC, patients with hypermethylation of two or three genes had a worse 5-year disease-free survival rate than those with hypermethylation of one or none of the three genes. Subgroup analysis showed that FLI1 hypermethylation in both tissue and plasma samples was associated with liver metastasis in MSI−/EBV− GC, and MSC hypermethylation in tissue samples was correlated with liver metastasis in MSI+ or EBV+ GC. Patients with FLI1 hypermethylation in plasma samples had a significantly worse 5-year disease-free survival rate than those without FLI1 hypermethylation in MSI−/EBV− GC. FLI1 hypermethylation was an independent prognostic factor affecting the overall survival and disease-free survival in both tissue and plasma samples.

Conclusions
DNA methylation is a useful biomarker for predicting tumor recurrence patterns and GC patient survival.
Satisfaction of Nigerian patients with health services: a protocol for a systematic review
Authors: Akunne Maureen O. , Okonta Mathew J. , Ukwe Chinwe V. , Heise Thomas L. , Ekwunife Obinna I. 
Published: November 2019

AbstractBackgroundPatient-based assessment of health services is becoming popular in measuring the standard of care. Both quantitative and qualitative methods are available. Patient satisfaction surveys are commonly used to record the experiences of patients in hospitals, whereas qualitative designs (e.g., interviews and focus group discussions) are used less frequently. To date, there has been no systematic review published devoted to patient satisfaction with health services in Nigeria. We aim to (1) systematically analyze relevant quantitative studies to pinpoint excellent procedures in measuring patient satisfaction with health services, (2) to investigate if a reference method (gold standard method) exists, and (3) to identify relevant topics which are recognized by patients as important for the delivery of a high-quality health service in Nigeria.MethodsSearches of eight electronic journal databases, including MEDLINE, EMBASE, CINAHL, PsycINFO, AJOL, CDSR, DARE, and HTA will be conducted to identify studies assessing patient satisfaction with health services in Nigeria. The searches will be supported by manual searches in reference lists of relevant primary studies and systematic reviews. The review will be limited to studies published since 2007. After a stepwise screening process by two reviewers, data from included studies will be extracted and reviewed. The COSMIN RoB checklist will be used to critically appraise included studies. We will carry out an extensive data synthesis to answer the review questions.DiscussionThe intended systematic review will provide information on how the satisfaction of patients with health services has earlier been described and assessed in Nigerian studies. It will establish if a gold standard method exists and synthesize information on topics which might be of special interest to patients. Review findings will enrich the debate on patient-centered care and overall performance of health quality standards in Nigeria.Systematic review registrationPROSPEROCRD42018108140
An automated fruit harvesting robot by using deep learning
Authors: Onishi Yuki , Yoshida Takeshi , Kurita Hiroki , Fukao Takanori , Arihara Hiromu , Iwai Ayako 
Published: November 2019

Abstract
Automation and labor saving in agriculture have been required recently. However, mechanization and robots for growing fruits have not been advanced. This study proposes a method of detecting fruits and automated harvesting using a robot arm. A highly fast and accurate method with a Single Shot MultiBox Detector is used herein to detect the position of fruit, and a stereo camera is used to detect the three-dimensional position. After calculating the angles of the joints at the detected position by inverse kinematics, the robot arm is moved to the target fruit’s position. The robot then harvests the fruit by twisting the hand axis. The experimental results showed that more than 90% of the fruits were detected. Moreover, the robot could harvest a fruit in 16 s.
Brittle to quasi-brittle transition and crack initiation precursors in crystals with structural Inhomogeneities
Authors: Papanikolaou S. , Shanthraj P. , Thibault J. , Woodward C. , Roters F. 
Published: November 2019

AbstractCrack initiation emerges due to a combination of elasticity, plasticity, and disorder, and it displays strong dependence on the material’s microstructural details. The characterization of the structural uncertainty in the original microstructure is typically empirical and systematic characterization protocols are lacking. In this paper, we propose an investigational tool in the form of the curvature of an ellipsoidal notch: As the radius of curvature at the notch increases, there is a dynamic phase transition from notch-induced crack initiation to disorder-induced crack nucleation. We argue that the this transition may unveil the characteristic length scale of structural disorder in the material. We investigate brittle but elastoplastic metals with continuum, microstructural disorder that could originate in a manufacturing process, such as alloying. We perform extensive and realistic simulations, using a phase-field approach coupled to crystal plasticity, where microstructural disorder and notch width are systematically varied. We identify the brittle-to-quasi-brittle transition for various disorder strengths in terms of the damage and stress evolution. Moreover, we investigate precursors to crack initiation that we quantify in terms of the expected stress drops during displacement control loading.
Outcome evaluation by patient reported outcome measures in stroke clinical practice (EPOS) protocol for a prospective observation and implementation study
Authors: Rimmele D. Leander , Lebherz Lisa , Frese Marc , Appelbohm Hannes , Bartz Hans-Jürgen , Kriston Levente , Gerloff Christian , Härter Martin , Thomalla Götz 
Published: November 2019

AbstractIntroductionThe impact of stroke-related impairment on activities of daily living may vary between patients, and can only be estimated by applying patient-reported outcome measures. The International Consortium for Health Outcome Measurement has developed a standard set of instruments that combine clinical and longitudinal patient-reported outcome measures for stroke. The present study was designed (1) to implement and evaluate the feasibility of the use of it as a consistent outcome measure in clinical routine at the stroke center of a German university hospital, (2) to characterize impairment in everyday life caused by stroke, and (3) to identify predictive factors associated with patient-relevant outcomes.MethodsWe plan to enroll 1040 consecutive patients with the diagnosis of acute ischemic stroke, transient ischemic attack, or intracerebral hemorrhage in a prospective observational study. Demographics, cardiovascular risk factors, and living situation are assessed at inpatient surveillance. At 90 days and 12 months after inclusion, follow-up assessments take place including the Patient-reported Outcomes Measurement Information System 10-Question Short Form (PROMIS-10), the Patient- Health Questionnaire-4, and the simplified modified Ranking Scale questionnaire. The acceptance and feasibility (1) will be assessed by a process evaluation through qualitative semi-structured interviews with clinical staff and patients and quantitative analyses of the data quality evaluating practicability, acceptance, adoption, and fidelity to protocol. The primary outcome of objective 2 and 3 is health-related quality of life measured with the PROMIS-10. Additional outcomes are depressive and anxiety symptoms and patient participation in their social roles. Patient-reported outcomes will be assessed in their longitudinal course using (generalized) mixed regressions. Exploratory descriptive and inference statistical analyses will be used to find patterns of patient characteristics and predictive factors of the outcome domains.PerspectiveThe results will describe and further establish the evaluation of stroke patients of a stroke center by standardized PROMs in everyday life.Trial registrationThe trial is registered atClinicalTrials.gov(NCT03795948). Approval of the local ethics committee (Ethik-Kommission der Ärztekammer Hamburg) has been obtained.
Heat shock protein 70 increases cell proliferation, neuroblast differentiation, and the phosphorylation of CREB in the hippocampus
Authors: Kwon Hyun Jung , Kim Woosuk , Jung Hyo Young , Kang Min Soo , Kim Jong Whi , Hahn Kyu Ri , Yoo Dae Young , Yoon Yeo Sung , Hwang In Koo , Kim Dae Won 
Published: November 2019

AbstractIn the present study, we investigated the effects of heat shock protein 70 (HSP70) on novel object recognition, cell proliferation, and neuroblast differentiation in the hippocampus. To facilitate penetration into the blood–brain barrier and neuronal plasma membrane, we created a Tat-HSP70 fusion protein. Eight-week-old mice received intraperitoneal injections of vehicle (10% glycerol), control-HSP70, or Tat-HSP70 protein once a day for 21 days. To elucidate the delivery efficiency of HSP70 into the hippocampus, western blot analysis for polyhistidine was conducted. Polyhistidine protein levels were significantly increased in control-HSP70- and Tat-HSP70-treated groups compared to the control or vehicle-treated group. However, polyhistidine protein levels were significantly higher in the Tat-HSP70-treated group compared to that in the control-HSP70-treated group. In addition, immunohistochemical study for HSP70 showed direct evidences for induction of HSP70 immunoreactivity in the control-HSP70- and Tat-HSP70-treated groups. Administration of Tat-HSP70 increased the novel object recognition memory compared to untreated mice or mice treated with the vehicle. In addition, the administration of Tat-HSP70 significantly increased the populations of proliferating cells and differentiated neuroblasts in the dentate gyrus compared to those in the control or vehicle-treated group based on the Ki67 and doublecortin (DCX) immunostaining. Furthermore, the phosphorylation of cAMP response element-binding protein (pCREB) was significantly enhanced in the dentate gyrus of the Tat-HSP70-treated group compared to that in the control or vehicle-treated group. Western blot study also demonstrated the increases of DCX and pCREB protein levels in the Tat-HSP70-treated group compared to that in the control or vehicle-treated group. In contrast, administration of control-HSP70 moderately increased the novel object recognition memory, cell proliferation, and neuroblast differentiation in the dentate gyrus compared to that in the control or vehicle-treated group. These results suggest that Tat-HSP70 promoted hippocampal functions by increasing the pCREB in the hippocampus.
Enhancing ecological-economic efficiency of intensive shrimp farm through in-out nutrient budget and feed conversion ratio
Authors: Chaikaew Pasicha , Rugkarn Natcha , Pongpipatwattana Varot , Kanokkantapong Vorapot 
Published: November 2019

Abstract
In aquaculture systems, insufficient nutrients impede shrimp growth while excessive amounts of nutrient inputs lead to environmental degradation and unnecessary high investment. A study of in-out nutrient budgets in an intensive Litopenaeus vannamei farm was conducted in this work to measure the amount of nitrogen (N) and phosphorus (P) input and output from the system. The feed conversion ratio (FCR) was obtained to determine the level of nutrient input performance. Between September and December 2017, monthly water and sediment samples were taken within one crop cycle. Nutrient concentrations in sediment and water increased over 90 days. The total nitrogen concentration in the pond water and effluents were in accordance with wastewater quality control for aquaculture; however, the total phosphorus concentration failed to meet the water quality control from the water input through the end of the crop cycle. The nutrient budget model showed that the input/output contained 107.8 kg N and 178.4 kg P. Most of the N input came from shrimp diets (80%) while most of the P came from fertilizer (57%). Both N (46%) and P (54%) mainly deposited in the sediment as an output process. The FCR of this farm is 2.0. Based on the 1.8 FCR scenario, this farm could reduce 147 kg of feed in total, which accounts for 9.04 kg N and 2.21 kg P reduction. The farmer could save $1027 USD crop− 1 ($1 USD = 32.7 baht), as well as reducing uneaten food at the bottom of the pond. This study offers a simple and straightforward model that can practically reduce environmental impacts and increase the profitability of farms.
Association between dietary sodium, calcium, saturated fat and blood pressure in obese Egyptian adolescents
Authors: Hassana Nayera E. , El Shebinib Salwa M. , El-Masrya Sahar A. , Ahmedb Nihad H. , Alia Manal M. , El-Saeedc Gamila S. M. , El-Lebedyd Dalia 
Published: November 2019

AbstractBackgroundOverweight/obesity, high dietary fat, sodium and low calcium intake are recognized as risk factors for primary hypertension in obese adults, children and adolescents.AimThe aim of this work was to assess the association between some dietary factors (fat, sodium, calcium) and blood pressure (BP) among obese Egyptian adolescents.Subjects and methodsSeventy-seven adolescents shared in this study. Their height and weight were reported. Body mass index (BMI) was calculated. Blood pressure was measured; the mean values were corrected for age and sex in the form of centile. Dietary interview consisting of 24 h recall was performed. Fasting blood glucose and serum lipid profile were determined.ResultsData revealed that, the percent of the prehypertensive was 42.86% and 38.80%, for boys and girls, no hypertensive cases were recorded. The mean values of their weight, DBP, and the serum lipid parameters were high compared to their controls; significant differences were found between the daily intake of calories, protein, carbohydrate, dietary fiber and cholesterol at p ≤ 0.05. The mean values of the daily intake of the vitamins and minerals were low in all groups compared to the RDAs, except sodium intake which was high in the prehypertensive boys and normal and prehypertensive girls.ConclusionThis study showed a high incidence of pre-hypertension among obese Egyptian adolescents, which is associated with high intake of sodium, saturated fat and cholesterol, along with low intake of calcium.
The Soviet Project of the 1930s to Found a “World Literature” and British Literary Internationalism
Authors: Clark Katerina 
Published: November 2019

Abstract
A major lacuna in Pascale Casanova’s account of world literature in her World Republic of Letters is the Soviet venture into establishing a “world literature” (mirovaia literatura) to be centered not in Paris but in Moscow. This aim was most actively pursued between the wars, when many writers were implicated in its international network. This moment in literary history provides a missing link in the progression from the more elitist world literature as conceived by Goethe and others in the early nineteenth century to world literature in our postcolonialist present and era of globalization. This article outlines the networks that sought to foster such a world literature and the main aesthetic controversies within the movement. In particular, the article looks at the efforts of such official spokesmen as Andrei Zhdanov, Karl Radek, and Georg Lukács to proscribe “bourgeois” modernism. It takes members of the British Writers’ International and their associated journals the Left Review and New Writing as case studies in the interplay between Moscow as putative “metropole” and the “periphery.”
Literary History after Literary Dominance
Authors: Hayot Eric 
Published: November 2019

Abstract
The various pronouncements of the nation’s dissolution seem to have been premature. Literary history is still very much within the nation, especially if one considers the realm of the middle- and lowbrow, or indeed the vast swaths of genre fiction. What has changed in literary history is the position of literature itself. The discipline of literary study (whether one thinks of it as literary history or literary criticism) institutionalized itself during a period of literary dominance. Now that that dominance is over—now that the field of narrative aesthetic culture includes television, film, and video games, and now that those genres dominate not only markets but the forms of representativity that used to belong almost exclusively to literature—what is the future for literary studies, either as a scholarly discipline or as an institutional field?
When More Is Not Better: Three Common Mistakes in Health Messaging Interventions
Authors: Farrow Katherine , Grolleau Gilles , Mzoughi Naoufel 
Published: November 2019

AbstractHealth messaging interventions frequently make three well-intentioned but mistaken choices in their communications strategies. To increase their persuasiveness, these messages frequently call attention to the greatest possible numbers of people engaging in undesirable behavior, victims of this behavior, and reasons why one should change the behavior. We raise recent research suggesting how and why the intuitively attractive more-is-better heuristic can be unproductive, and suggest ways to overcome these pitfalls.
Model systems for regeneration: Hydra
Authors: Vogg Matthias C. , Galliot Brigitte , Tsiairis Charisios D. 
Published: November 2019

ABSTRACT
The freshwater polyp Hydra provides a potent model system for investigating the conditions that promote wound healing, reactivation of a developmental process and, ultimately, regeneration of an amputated body part. Hydra polyps can also be dissociated to the single cell level and can regenerate a complete body axis from aggregates, behaving as natural organoids. In recent years, the ability to exploit Hydra has been expanded with the advent of new live-imaging approaches, genetic manipulations that include stable transgenesis, gene silencing and genome editing, and the accumulation of high-throughput omics data. In this Primer, we provide an overview of Hydra as a model system for studying regeneration, highlighting recent results that question the classical self-enhancement and long-range inhibition model supposed to drive Hydra regeneration. We underscore the need for integrative explanations incorporating biochemical as well as mechanical signalling.