bluuebunny/crossref_metadata_2025_split huggingface dataset:
Immunologic perturbations in severe COVID-19/SARS-CoV-2 infection
Authors: Kuri-Cervantes Leticia , Pampena M. Betina , Meng Wenzhao , Rosenfeld Aaron M. , Ittner Caroline A.G. , Weisman Ariel R. , Agyekum Roseline , Mathew Divij , Baxter Amy E. , Vella Laura , Kuthuru Oliva , Apostolidis Sokratis , Bershaw Luanne , Dougherty Jeannete , Greenplate Allison R. , Pattekar Ajinkya , Kim Justin , Han Nicholas , Gouma Sigrid , Weirick Madison E. , Arevalo Claudia P. , Bolton Marcus J. , Goodwin Eileen C. , Anderson Elizabeth M. , Hensley Scott E. , Jones Tiffanie K. , Mangalmurti Nilam S. , Luning Prak Eline T. , Wherry E. John , Meyer Nuala J. , Betts Michael R. 
Published: May 2020

AbstractAlthough critical illness has been associated with SARS-CoV-2-induced hyperinflammation, the immune correlates of severe COVID-19 remain unclear. Here, we comprehensively analyzed peripheral blood immune perturbations in 42 SARS-CoV-2 infected and recovered individuals. We identified broad changes in neutrophils, NK cells, and monocytes during severe COVID-19, suggesting excessive mobilization of innate lineages. We found marked activation within T and B cells, highly oligoclonal B cell populations, profound plasmablast expansion, and SARS-CoV-2-specific antibodies in many, but not all, severe COVID-19 cases. Despite this heterogeneity, we found selective clustering of severe COVID-19 cases through unbiased analysis of the aggregated immunological phenotypes. Our findings demonstrate broad immune perturbations spanning both innate and adaptive leukocytes that distinguish dysregulated host responses in severe SARS-CoV-2 infection and warrant therapeutic investigation.One Sentence SummaryBroad immune perturbations in severe COVID-19
Molecular mapping of qBK1Z, a major QTL for bakanae disease resistance in rice
Authors: Lee Sais-Beul , Kim Namgyu , Jo Sumin , Hur Yeon-Jae , Lee Ji-youn , Cho Jun-Hyeon , Lee Jong-Hee , Kang Ju-Won , Song You-Chun , Bombay Maurene , Kim Sung-Ryul , Lee Jungkwan , Seo Young-Su , Ko Jong-Min , Park Dong-Soo 
Published: May 2020

AbstractBakanae disease is a fungal disease of rice (Oryza sativa L.) caused by the pathogen Gibberella fujikuroi (also known as Fusarium fujikuroi). Recently the disease incidence has increased in several Asian countries and continues to spread throughout the world. No rice varieties have been developed yet to be completely resistant to this disease. With increasing need to identify various genetic resources to impart resistance to local elite varieties, this study was carried out to identify novel quantitative trait loci (QTLs) from an indica variety Zenith. We performed a QTL mapping using 180 F2:9 recombinant inbred lines (RILs) derived from a cross between the resistant variety, Zenith, and the susceptible variety, Ilpum. A primary QTL study using the genotypes and phenotypes of the RILs indicated that the locus qBK1z conferring bakanae disease resistance from the Zenith was located in a 2.8 Mb region bordered by the two SSR markers, RM1331 and RM3530 on chromosome 1.The log of odds (LOD) score of qBK1z was 13.43, accounting for 30.9% of the total phenotypic variation. A finer localization of qBK1z was delimited at an approximate 730 kb interval in the physical map between Chr01_1435908 (1.43 Mbp) and RM10116 (2.16 Mbp). The development of a rice variety with a higher level of resistance against bakanae disease is a major challenge in many rice growing countries. Introducing qBK1z or pyramiding with other previously identified QTLs could provide effective genetic control of bakanae disease in rice.
The transcriptome ofListeria monocytogenesduring co-cultivation with cheese rind bacteria suggests adaptation by induction of ethanolamine and 1,2-propanediol catabolism pathway genes
Authors: Anast Justin M. , Schmitz-Esser Stephan 
Published: May 2020

AbstractThe survival ofListeria (L.) monocytogenesin foods and food production environments (FPE) is dependent on several genes that increase tolerance to stressors; this includes competing with intrinsic bacteria. We aimed to uncover genes that are differentially expressed (DE) inL. monocytogenessequence type (ST) 121 strain 6179 when co-cultured with cheese rind bacteria.L. monocytogeneswas cultivated in broth or on plates with either aPsychrobacterorBrevibacteriumisolate from cheese rinds. RNA was extracted from co-cultures in broth after two or 12 hours and from plates after 24 and 72 hours. Broth co-cultivations withBrevibacteriumorPsychrobacteryielded up to 392 and 601 DE genes, while plate co-cultivations significantly affected the expression of up to 190 and 485L. monocytogenesgenes, respectively. Notably, the transcription of virulence genes encoding theListeriaadhesion protein and Listeriolysin O were induced during plate and broth co-cultivations. The expression of several systems under the control of the global stress gene regulator, σB, increased during co-cultivation. A cobalamin-dependent gene cluster, responsible for the catabolism of ethanolamine and 1,2-propanediol, was upregulated in both broth and plate co-cultures conditions. Finally, a small non-coding (nc)RNA, Rli47, was induced after 72 hours of co-cultivation on plates and accounted for 50-90% of the total reads mapped toL. monocytogenes. A recent study has shown that Rli47 may contribute toL. monocytogenesstress survival by slowing growth during stress conditions through the suppression of branch-chained amino acid biosynthesis. We hypothesize that Rli47 may have an impactful role in the response ofL. monocytogenesto co-cultivation by regulating a complex network of metabolic and virulence mechanisms.
PU.1 enforces quiescence and limits hematopoietic stem cell expansion during inflammatory stress
Authors: Chavez James S. , Rabe Jennifer L. , Loeffler Dirk , Higa Kelly C. , Hernandez Giovanny , Mills Taylor S. , Ahmed Nouraiz , Gessner Rachel L. , Ke Zhonghe , Idler Beau M. , Kim Hyun Min , Myers Jason R. , Stevens Brett M. , Jordan Craig T. , Nakajima Hideaki , Ashton John , Welner Robert S. , Schroeder Timm , DeGregori James , Pietras Eric M. 
Published: May 2020

SummaryLoss of hematopoietic stem cell (HSC) quiescence and resulting clonal expansion are common initiating events in the development of hematological malignancy. Likewise, chronic inflammation related to aging, disease and/or tissue damage is associated with leukemia progression, though its role in oncogenesis is not clearly defined. Here, we show that PU.1-dependent repression of protein synthesis and cell cycle genes in HSC enforces homeostatic protein synthesis levels and HSC quiescence in response to IL-1 stimulation. These genes are constitutively de-repressed in PU.1-deficient HSC, leading to activation of protein synthesis, loss of quiescence and aberrant expansion of HSC. Taken together, our data identify a mechanism whereby HSC regulate their cell cycle activity and pool size in response to chronic inflammatory stress.
Protein context shapes the specificity of domain-peptide interactionsin vivo
Authors: Dionne Ugo , Bourgault Émilie , Dubé Alexandre K , Bradley David , Chartier François JM , Dandage Rohan , Dibyachintan Soham , Després Philippe C , Gish Gerald D , Lambert Jean-Philippe , Bisson Nicolas , Landry Christian R 
Published: May 2020

AbstractProtein-protein interactions (PPIs) between modular binding domains and their target peptide motifs are thought to largely depend on the intrinsic binding specificities of the domains. By combining deletion, mutation, swapping and shuffling of SRC Homology 3 (SH3) domains and measuring their impact on protein interactions, we find that most SH3s do not autonomously dictate PPI specificityin vivo. The identity of the host protein and the position of the SH3 domains within their host are both critical for PPI specificity, for cellular functions and for key biophysical processes such as phase separation. Our work demonstrates the importance of the interplay between a modular PPI domain such as SH3 and its host protein in establishing specificity to wire PPI networks.
Numerical predictions of the flow characteristics of subsonic jet emanating from corrugated lobed nozzle
Authors: V. Paul Roy , K.G. Kriparaj , P.S. Tide 
Published: May 2020

Purpose
The purpose of this study is to investigate the aerodynamic characteristics of subsonic jet emanating from corrugated lobed nozzle.

Design/methodology/approach
Numerical simulations of subsonic turbulent jets from corrugated lobed nozzles using shear stress transport k-ω turbulence model have been carried out. The analysis was carried out by varying parameters such as lobe length, lobe penetration and lobe count at a Mach number of 0.75. The numerical predictions of axial and radial variation of the mean axial velocity, u′u′ ¯ and v′v′ ¯ have been compared with experimental results of conventional round and chevron nozzles reported in the literature.

Findings
The centreline velocity at the exit of the corrugated lobed nozzle was found to be lower than the velocity at the outer edges of the nozzle. The predicted potential core length is lesser than the experimental results of the conventional round nozzle and hence the decay in centreline velocity is faster. The centreline velocity increases with the increase in lobe length and becomes more uniform at the exit. The potential core length increases with the increase in lobe count and decreases with the increase in lobe penetration. The turbulent kinetic energy region is narrower with early appearance of a stronger peak for higher lobe penetration. The centreline velocity degrades much faster in the corrugated nozzle than the chevron nozzle and the peak value of Reynolds stress appears in the vicinity of the nozzle exit.

Practical implications
The corrugated lobed nozzles are used for enhancing mixing without the thrust penalty inducing better acoustic benefits.

Originality/value
The prominent features of the corrugated lobed nozzle were obtained from the extensive study of variation of flow characteristics for different lobe parameters after making comparison with round and chevron nozzle, which paved the way to the utilization of these nozzles for various applications.
A pragmatic mixed-methods review of changing “case-complexity” of referrals to an intensive support service
Authors: Clifford Adam , Kemp Francesca Georgina 
Published: May 2020

Purpose
“Case-complexity” is a widely used but under-explored concept across health and social care. A region’s Intensive Support Teams (ISTs) had been reporting an increase in “case-complexity”, but had not tested this hypothesis against data. This study aims to investigate this question through a pragmatic mixed-methods approach as part of a wider service evaluation.

Design/methodology/approach
Health of the Nation Outcome Scales for People with Learning Disabilities (HoNOS-LD) scores were used (n = 1,766) to estimate average “case-complexity” of referrals over an eight-year sample period. Two focus groups for IST staff (n = 18) explored why “case-complexity” appears to be increasing. Participant perspectives were subjected to thematic analysis.

Findings
Average HoNOS-LD scores have steadily increased over the sample period, suggestive of increasing “case-complexity”. Focus groups identified three broad themes to potentially explain the increased complexity: effects of Transforming Care; people’s changing and unchanging support systems; and issues related to mild and borderline intellectual disability. Many perspectives are grounded in or supported by evidence.

Research limitations/implications
Implications and limitations of findings are discussed, including areas for further consideration and research. The well-designed “short-cut” is promoted as a strategy for busy professionals in need of practice-based evidence but with limited research time and resources.

Originality/value
The findings and discussion will be of value to anyone involved in the design, commissioning and delivery of mental health and challenging behaviour services to people with intellectual and developmental disabilities (IDD) under Transforming Care. Study methodology is easily replicable to build broader picture about “case-complexity” among UK’s IDD population.
Factors influencing marketing channel choices for improved indigenous chicken farmers: insights from Baringo, Kenya
Authors: Kiprop Emmanuel Kiprotich , Okinda Cedric , Akter Asma , Geng Xianhui 
Published: May 2020

PurposeImproved indigenous chicken is considered a sustainable agricultural practice with social, economic and environmental indicators. Therefore, the analysis of the choice of market channels is of considerable importance to farmers with reference to improved livelihoods and poverty alleviation in developing countries. The purpose of this study is to investigate the factors that influence market channel choices among improved indigenous chicken farmers in Baringo County and to rank the determinants according to their level of importance in influencing farmer's choice of marketing channels.Design/methodology/approachA multistage sampling technique was employed to collect data from 209 households for the study conducted between April and July 2019, out of which, 198 useful responses were obtained. Multinomial logit regression and neural network models were used to analyze the factors influencing market channel choice based on socioeconomic, demographic and farm characteristics.FindingsIt was established that group membership, education, market distance, transport costs, farm size, cost of information and bargain costs were statistically significant in the choice of market channels (wholesaler, brokers, processors and supermarkets). With the direct consumer as the base market choice. The cost of transport had the highest normalized importance in the prediction of a farmer's selection of market channels for both radial basis function (RBF) and multilayer perceptron (MLP) neural networks. However, flock attributes and age of household head had the least normalized importance in MLP and RBF, respectively.Research limitations/implicationsDue to the insufficiency of resources and time, this study only focused on a small part of the country (Baringo County). However, improved indigenous chicken farming is widely practiced in Kenya. Further studies can be carried out in other counties to validate the results of this study.Practical implicationsThe outcome can be used in policy implementation involving improved indigenous chicken production in Kenya.Originality/valueThis study suggests the methods aimed at enhancing poultry sector in other counties in Kenya as well as other developing countries.
Artificial intelligence exploitation in facility management using deep learning
Authors: Marzouk Mohamed , Zaher Mohamed 
Published: May 2020

Purpose
This paper aims to apply a methodology that is capable to classify and localize mechanical, electrical and plumbing (MEP) elements to assist facility managers. Furthermore, it assists in decreasing the technical complexity and sophistication of different systems to the facility management (FM) team.

Design/methodology/approach
This research exploits artificial intelligence (AI) in FM operations through proposing a new system that uses a deep learning pre-trained model for transfer learning. The model can identify new MEP elements through image classification with a deep convolutional neural network using a support vector machine (SVM) technique under supervised learning. Also, an expert system is developed and integrated with an Android application to the proposed system to identify the required maintenance for the identified elements. FM team can reach the identified assets with bluetooth tracker devices to perform the required maintenance.

Findings
The proposed system aids facility managers in their tasks and decreases the maintenance costs of facilities by maintaining, upgrading, operating assets cost-effectively using the proposed system.

Research limitations/implications
The paper considers three fire protection systems for proactive maintenance, where other structural or architectural systems can also significantly affect the level of service and cost expensive repairs and maintenance. Also, the proposed system relies on different platforms that required to be consolidated for facility technicians and managers end-users. Therefore, the authors will consider these limitations and expand the study as a case study in future work.

Originality/value
This paper assists in a proactive manner to decrease the lack of knowledge of the required maintenance to MEP elements that leads to a lower life cycle cost. These MEP elements have a big share in the operation and maintenance costs of building facilities.
Crisis situations: how should micro, small and medium enterprises handle them with a long term view?
Authors: Csath Magdolna 
Published: May 2020

Purpose
Micro, Small and Medium Enterprises (MSMEs) are the backbone of economies. They employ about 70 percent of the working age population and produce on average about half of new value-added. Self-employed micro’s are a special segment of the MSME population. They are either the typical service providers or the new start-ups, later becoming rapidly growing gazelles. It is therefore very important that the majority of these businesses survive crises. There are plenty of suggestions about what governments should do to help, but what should MSMEs themselves do, not only for survival but also for a successful future? This is the topic of this article.

Design/Methodology/Approach
The article is based on international research findings and the long-time professional experience of the author on the operations of the MSME sector in an international context.

Findings
MSMEs can survive and even thrive on challenges created by sudden crisis situations, like a pandemic, if they have a proactive managerial mentality and also diverse professional knowledge, often called intellectual capital. There are well-known, but perhaps now neglected, management techniques which can support this combination of short and long run orientation, including future building aspirations. Combined they can enhance the intellectual capital stock of business, serving as a springboard for future sustainable success.

Originality/value
Crisis situations do not happen – fortunately - too often. Therefore. experiments about how to handle them, especially in the MSME sector are not extensively available. This paper suggests a tool-set which might be especially useful not only for handling crisis situations, but also for improving chances for lasting business success.
Status quo bias and shoppers’ mobile website purchasing resistance
Authors: Nel Jacques , Boshoff Christo 
Published: May 2020

Purpose
Shopping statistics indicate that online shoppers prefer purchasing products using the desktop website of the retailer, rather than using the mobile website on a mobile phone to purchase products (mobile website purchasing). Therefore, using status quo bias theory, this study aims to investigate mobile website purchasing resistance of those customers using only desktop website purchasing.

Design/methodology/approach
To test the conceptual model an online questionnaire was used to collect data from customers purchasing products using only the desktop website on a computer (n = 484) and not the retailer’s mobile website.

Findings
Due to cognitive dissonance, customers using only desktop purchasing trivialize mobile website purchasing perceived attractiveness while perceiving more cognitive effort in mobile website purchasing to maintain consonance with their inertia. Further, relative advantage perceptions of mobile website purchasing lead to more trivialization of mobile website purchasing attractiveness perceptions. Desktop purchasing inertia enhances resistance through alternative attractiveness and cognitive effort perceptions, respectively, and cognitive effort and alternative attractiveness perceptions in serial. Desktop purchasing habit has the strongest positive influence on desktop purchasing inertia.

Research limitations/implications
This study was conducted in a high-involvement product context. Replication in a low-involvement product context is necessary to confirm the robustness of the results.

Practical implications
Retailers can use the findings to develop strategies to lower mobile website purchasing resistance in an online-mobile concurrent channel environment.

Originality/value
The study provides novel insights into mobile website purchasing resistance in an online-mobile concurrent channel environment. Further, the study addresses the gap in research on inertia and switching costs in the adoption of concurrent channels.
Factors affecting successful transition between post-disaster recovery phases: a case study of 2010 floods in Sindh, Pakistan
Authors: Ali Rizwan Akbar , Mannakkara Sandeeka , Wilkinson Suzanne 
Published: May 2020

Purpose
This paper aims to describe an in-depth study conducted on transition of recovery into subsequent recovery phases after the 2010 super floods in the Sindh province of Pakistan. The objectives of this research were to examine the post-disaster activities after the floods and highlight the critical areas hindering the transition into an effective recovery phase.

Design/methodology/approach
A case study approach based on literature reviews with semi-structured interviews with disaster management stakeholders were applied as the primary source of data.

Findings
The study found that long-term recovery was the most neglected phase of post-disaster recovery (PDR). The factors hindering successful transition following short-term recovery activities are lack of following: community-level involvement, local administration and community capacity, disaster governance, different stakeholders and coordination, information and knowledge management.

Research limitations/implications
This paper examines the long-term disaster recovery after the 2010 super floods in three districts of Sindh. Therefore, researchers are encouraged to investigate the factors in other areas for different types of disasters.

Practical implications
These findings are critical to planning future post-disaster recoveries in the region. It also provides a basis to investigate other types of disasters.

Originality/value
The transition of recovery into long-term phase has never been investigated before. The recovery phase is an opportune time to incorporate strategies for building back better, resilience, mitigation and preparedness. A PDR that does not incorporate these strategies in the long-term leaves affected communities in more vulnerable conditions for future disasters.