bluuebunny/crossref_metadata_2025_split huggingface dataset:
Research on Personal Credit Evaluation of Internet Finance Based on Blockchain and Decision Tree Algorithm
Authors: Zhao Yuhao 
Published: August 2020

Abstract
With the development of Internet finance, existing financial platforms have gradually formed a large-scale, dynamic operating environment. How to ensure information security and realize personal credit evaluation is an urgent problem to be solved in the development of Internet financial platforms. The rise of blockchain technology has provided new solutions for the management of Internet financial platforms and information security. In view of the shortcomings of the current Internet financial credit evaluation, this article discusses the key standards of personal credit evaluation. With the help of blockchain, decision tree and other technologies, this paper designs the credit evaluation process and establishes personal credit evaluation technology. Experiments and analysis show that this technology can effectively improve the transparency of personal credit information in Internet finance. This technology is used to study credit risk assessment factors and provide new solutions for the intelligent transformation and upgrading of Internet finance.
Production of dengue virus-like particles serotype-3 in silkworm larvae and their ability to elicit a humoral immune response in mice
Authors: Umoto Doddy Irawan Setyo , Pambudi Sabar , Sjatha Fithriyah , Kato Tatsuya , Park Enoch Y. 
Published: August 2020

Abstract
To develop monovalent dengue virus-like particle for serotype 3 (DENV-LP/3), we prepared and expressed two structural polyprotein constructs using silkworm and Bm5 cells: DENV-3 Capsid-premembrane-envelope (DENV-3CprME) and premembrane-envelope (DENV-3prME). The expressed PA-tagged 3CprME and 3prME polypeptides were partially purified by PA-tag affinity chromatography and had molecular weights of 85 and 75 kDa, respectively. Expressed proteins were separately verified using the following primary antibodies: the anti-PA tag antibody, DENV premembrane polyclonal antibody, and DENV envelope polyclonal antibody. Transmission electron microscopy revealed that these DENV-3CprME and 3prME formed rough, spherical DENV-LPs (DENV-LP/3CprME and DENV-LP/3prME), respectively, with a diameter of 30–55 nm. The heparin-binding assay demonstrated that these DENV-LPs contained the envelope protein domain III on their surfaces. Both DENV-LPs showed an affinity to sera from human dengue patients and immunized mice. Immunization of mice with DENV-LP/3prME significantly induced the level of antibodies compared with DENV-LP/3CprME. These results indicate that DENV-LP/3prME is suitable as a vaccine candidate compared with DENV-LP/3CprME.
Genomic Regions Associated with Important Seed Quality Traits in Food-grade Soybeans
Authors: Whiting Rachel M. , Torabi Sepideh , Lukens Lewis , Eskandari Mehrzad 
Published: August 2020

Abstract
Background: The production of soy-based food products requires specific physical and chemical characteristics of the soybean seed. Identification of quantitative trait loci (QTL) associated with value-added traits, such as seed weight, seed protein and sucrose concentration, could accelerate the development of competitive high-protein soybean cultivars for the food-grade market through marker-assisted selection (MAS). The objectives of this study were to identify and validate QTL associated with these value-added traits in two high-protein recombinant inbred line (RIL) populations.Results: The RIL populations were derived from the high-protein cultivar ‘AC X790P’ (49% protein, dry weight basis), and two high-yielding commercial cultivars, ‘S18-R6’ (41% protein) and ‘S23-T5’ (42% protein). Fourteen large-effect QTL (R 2 >10%) were identified associated with seed protein concentration. Of these QTL, seven QTL were detected in both populations, and eight of them were co-localized with QTL associated with either seed sucrose concentration or seed weight. None of the protein-related QTL was found to be associated with seed yield in either population. Sixteen candidate genes with putative roles in protein metabolism were identified within seven of these protein-related regions: qPro_Gm02-3, qPro_Gm04-4, qPro_Gm06-1, qPro_Gm06-3, qPro_Gm06-6, qPro_Gm13-4 and qPro-Gm15-3.Conclusion: The use of RIL populations derived from high-protein parents created an opportunity to identify four novel QTL that may have been masked by large-effect QTL segregating in populations developed from diverse parental cultivars. In total, we have identified nine protein QTL that were detected either in both populations in the current study or reported in other studies. These QTL may be useful in the curated selection of new soybean cultivars for optimized soy-based food products.
Relationship between health literacy and fast food consumption: A population-based study from southern Iran
Authors: Namdar Azam , Naghizadeh Mohammad Mehdi , Montazeri Ali , Zamani Marziyeh 
Published: August 2020

Abstract
Background: Having appropriate health literacy may affect the consumption of fast food. We aimed to evaluate the effect of health literacy on fast food consumption among the adult populations.Methods: We evaluated health literacy and fast food consumption using an appropriate questionnaire in 421 adult participants with the age range of 18-65 years old in Fasa city, Fars Province, southern Iran. Two-step cluster sampling and, then, systematic sampling were performed. Data collection was done through demographic information questionnaire, checklist on fast food consumption, and the Health Literacy for Iranian Adults (HELIA) questionnaire. Data were harvested by face-to-face conversations. Population data across groups with/without fast food intake were compared.Results: Most of our participants used fast food every few months (49.9%). People with low or unstable income consumed more fast food than others (P<0.05). Sandwich and hotdog were the most consumed fast food (60.8%) and pizza was in the next step (34.9%). Sausage and soda were the most seasoning food (66.7%). Most of our participants used fast food as dinner (67.9%) and along with their family (72.2%), suggesting the institutionalized consumption of this type of food in the family. Fun was the most frequent reason for the use of fast food (66.5%). Most of the participants completely knew about the raw materials for fast food and their adverse effects. Finally, we found that health literacy among those who used fast food was less than those who did not consumed fast food. This difference was also observed in the subscales of reading skills, critique, and decision-making.Conclusions: It seems differences in health literacy and its components can affect fast food consumption. In addition, there was a negative relationship between health literacy and fast food consumption, which was also observed in reading skills, critique, and decision-making.
Research on Sports Fitness Management Based on Blockchain and Internet of Things
Authors: Shan Yu , Mai Yuehui 
Published: August 2020

Abstract
The amount of exercise and fitness level of sports athletes can be quantitatively evaluated through the measurement of health and sports information, thereby ensuring effective fitness effects. The development of blockchain and Internet of Things technology provides a new perspective and technical means for fitness management technology. In view of the current problems in the field of sports fitness, this paper designs and implements a dynamic management technology for sports fitness based on the concept of Internet of Things and blockchain. First, based on an in-depth analysis of the current status of theoretical research and application of the Internet of Health at home and abroad, the theories and methods of sports information and health information collection are studied. A temperature sensor and an acceleration sensor are used to collect human body temperature and exercise steps, respectively, and then estimate human health and exercise conditions. Second, solve the privacy problem in the collection and transmission of the Internet of Things by adding blockchain technology. Finally, the machine learning method is used to clean and manage the information and data to realize the real-time detection and management of the athlete's fitness status. The actual case test shows that the functions and technical performance indicators of the dynamic fitness management technology can meet the needs of users in indoor and outdoor fitness management, and promote the development of the sports industry and provide a scientific reference.
The effects of varying doses of caffeine on cardiac parasympathetic reactivation following an acute bout of anaerobic exercise in recreational athletes
Authors: Sarshin Amir , Naderi Alireza , Cruz Carlos Janssen Gomes da , Feizolahi Foad , Forbes Scott C. , Candow Darren G. , Mohammadgholian Ebrahim , Amiri Mehrdad , Jafari Naghmeh , Rahimi Alireza , Alijani Eidi , Earnest Conrad P. 
Published: August 2020

Abstract
Background: To examine the effects of varying doses of caffeine on autonomic reactivation following anaerobic exercise. Methods: Recreationally active males (N=20; 24±2y) participated in a randomized, double-blind, placebo-controlled, crossover study where participants ingested: (1) Control (CON; no supplement), (2) a non-caffeinated placebo (PLA), (3) 3-mg∙kg-1 of caffeine (CAF3) or (4) 6-mg∙kg-1 of caffeine (CAF6) prior to Wingate testing. Parasympathetic (lnRMSSD, primary outcome) and global HRV (lnSDNN, secondary outcome) were assessed at rest (i.e., pre-ingestion), 45-min post-ingestion, and 5-min and 35-min post-exercise recovery. We used a GLM to assess mean (95% CI) changes from pre-ingestion baseline.Results: Overall, we observed a significant trend for lnRMSSD and lnSDNN (both, p=0.001, ηp2=0.745). Forty-five minutes after treatment ingestion, we observed a significant increase in lnRMSSD for CAF3 (0.15ms, 95%CI, 0.07,0.24) and CAF6 (0.16ms, 95%CI, 0.06,0.25), both being significant (both, p<0.004) vs. CON (-0.02ms, 95%CI, -0.09,0.04). Five-minutes after exercise, all treatments demonstrated significant declines in lnRMSSD vs. baseline (all, p<0.001). After 35-min of recovery, lnRMSSD returned to a levels not significantly different than baseline for CAF3 (0.03ms, 95%CI, -0.05, 0.12) and CAF6 (-0.03ms, 95%CI, -0.17, 0.10), while PLA (-0.16ms, 95%CI, -0.25, -0.06) and CON (-0.17ms, 95%CI, -0.28, -0.07) treatments remained significantly depressed. A similar pattern was also observed for SDNN.Conclusion: Caffeine ingestion increases resting cardiac autonomic modulation and accelerates post-exercise autonomic recovery after a bout of an anaerobic exercise test in recreationally active young men. However, no differences between caffeine doses on cardiac autonomic reactivity were observed.
Conversion From Chronic To Episodic Migraine In Patients Treated With Erenumab: Real-Life Data From An Italian Region
Authors: Ornello Raffaele , Casalena Alfonsina , Frattale Ilaria , Caponnetto Valeria , Gabriele Amleto , Affaitati Giannapia , Giamberardino Maria Adele , Assetta Maurizio , Maddestra Maurizio , Marzoli Fabio , Viola Stefano , Cerone Davide , Marini Carmine , Pistoia Francesca , Sacco Simona 
Published: August 2020

Abstract
Background. Most patients treated with erenumab in clinical practice have chronic migraine (CM). We assessed the rate and possible predictors of conversion from CM to episodic migraine (EM) in a real-life study.Main body. We performed a subgroup analysis of patients treated with erenumab from January 2019 to February 2020 in the Abruzzo region, central Italy. Treatment was provided according to current clinical practice. For the purpose of the present study, we included patients fulfilling the definition of CM for the three months preceding erenumab treatment and with at least 6 months of follow-up after treatment. We assessed the rate of conversion to EM from baseline to Months 4-6 of treatment and during each month of treatment. To test the clinical validity of conversion to EM, we also assessed the decrease in monthly headache days (MHDs), acute medication days, and median headache intensity on a Numerical Rating Scale (NRS). We included in our study 91 patients with CM. At Months 4-6, 62 patients (68.1%) converted from CM to EM; the proportion of converters increased from Month 1 to Month 5. In the overall group of patients, median MHDs decreased from 26.5 (IQR 20-30) to 7.5 (IQR 5-16; P<0.001) compared with baseline, while median acute medication days decreased from 21 (IQR 16-30) to 6 (IQR 3-10; P<0.001) and median NRS scores decreased from 8 (IQR 7-9) to 6 (IQR 4-7; P<0.001). Significant decreases were found both in converters and in non-converters. We found no significant predictors of conversion to EM among the patients’ baseline characteristics.Conclusions. In our study, two thirds of patients with CM converted to EM during 6 months of treatment with erenumab. MHDs, acute medication use, and headache intensity decreased regardless of conversion from CM to EM.
In Silico Analysis of CadF Epitope-based Vaccine Design Against Campylobacter Jejuni
Authors: Naseri Mona Moballegh , Shams Saeed , Naseri Mohammad Moballegh , Bakhshi Bita 
Published: August 2020

Abstract
Objective: To eradicate infectious diseases, vaccination is an important strategy. CadF protein of Campylo bacter jejuni is one of the important factors in the process of the pathogenesis of the bacterium. So, the purpose of the work was to perform a bioinformatics study for identifying an epitope-based CadF vaccine, as a subunit vaccine. CadF sequences were extracted from the NCBI database. In silico analysis including all ergeni city, antigenicity, epitope conservancy assessment, molecular docking, etc.was done by different servers. Results:The results showed that CadF is an antigenic and non-allergenic protein and provides a suitable structure for vaccine design. Among epitopes, LSDSLALRL has been confirmed to stimulate both B and T cells. This 9-mers peptide is located in 135-143 segment of the CadF protein and interacted with HLA-A0101 and HLA-DRB1 0101 with energies of docking -26.18kcal/mol and -109.89kcal/mol. The peptide is not an allergen and as an antigen, it has the ability for motivating the immune system. Hence, the analyses are performed on that the epitope structure could verify the design of a vaccine against C. jejuni. The obtained theoretical results showed that CadF protein could be used for designing and evaluating a new vaccine in humans.
Specific Dynamic Variations in the Peripheral Blood Lymphocyte Subsets in COVID-19 and Severe Influenza a Patients: A Retrospective Observational Study
Authors: Qian Fang , Gao Guiju , Song Yangzi , Xu Yanli , Wang Aibin , Wang Sa , Yiwei Hao , Chen Meiling , Ma Xiaoyang , Zhao Tianwei , Guo Xiaodi , Chen Zhihai , Zhang Fujie 
Published: August 2020

Abstract
Background:
Both COVID-19 and influenza A contribute to increased mortality among the elderly and those with existing comorbidities. Changes in the underlying immune mechanisms determine patient prognosis. This study aimed to analyze the role of lymphocyte subsets in the immunopathogenesisof COVID-19 and severe influenza A, and examined the clinical significance of their alterations in the prognosis and recovery duration.
Methods:
By retrospectively reviewing of patients in four groups (healthy controls, severe influenza A, non-severe COVID-19 and severe COVID-19) who were admitted to Ditan hospital between 2018 to 2020, we performed flow cytometric analysis and compared the absolute counts of leukocytes, lymphocytes, and lymphocyte subsets of the patients at different time points (weeks 1–4).
Results:
We reviewed the patients’ data of 110 healthy blood donors, 80 Non-severe-COVID-19, 19 Severe-COVID-19 and 43 severe influenza A. We found total lymphocytes (0.93 × 109/L, 0.84 × 109/L vs 1.78 × 109/L, P < 0.0001) and lymphocyte subsets (T cells, CD4+ and CD8+ T cell subsets) of both severe patients to be significantly lower than those of healthy donors at early infection stages. Further, significant dynamic variations were observed at different time points (weeks 1–4).
Conclusions:
Our study indicates lymphopenia to be associated with disease severity and suggests the plausible role of lymphocyte subsets in disease progression, which in turn affects prognosis and recovery duration in patients with severe COVID-19 and influenza A.
Comparative study of a single posterior approach and combined posterior-anterior approach for patients with lower lumbar tuberculosis
Authors: Cui Yaqing , Gong Liqun , Zhou Yongchun , Duan Dapeng , Fan Yayi , Liu Jun , Li Weiwei 
Published: August 2020

Abstract
Background

To compare the surgical effect of a single posterior approach with a combined posterior-anterior approach for patients with lower lumbar tuberculosis (TB).
Method:

119 cases of lower lumbar TB were conducted from January 2008 to December 2016 using two procedures. Out of 119 cases, 73 patients were operated with a single posterior approach (group A), whereas, 46 patients were operated using the combined posterior-anterior approach (group B). The evaluative items were compared between two groups, including operation time, blood loss, surgical complication rate, cure rate, Visual Analog Scale (VAS) score, Japanese Orthopaedic Association (JOA) score, kyphosis Cobb’s angle, and time of abscess disappearance. Furthermore, the period between bone graft fusion and the patients’ return to regular activity were compared between the two groups to evaluate the therapeutic effects.
Results

No significant differences of gender, age, BMI, illness duration, and preoperative indexes of VAS, JOA, ESR, CRP, and kyphosis Cobb’s angle were found between the two groups of patients, who were followed-up for 24–60 months. Although the operation time and blood loss rate were less in group A as compared to group B, no remarkable differences in VAS, JOA, ESR, CRP and kyphosis Cobb’s angle were found. Furthermore, the rate of surgical complications, cure rate, and time of bone graft fusion did not show appreciable differences between the two groups. However, the time of abscess disappearance and time return to regular activity were less in group B as opposed to patients of group A.
Conclusion

Both single posterior approach and combined posterior-anterior approach are effective for the treatment of lower lumbar TB with a high cure rate and low surgical complication rate. Addtionally, single posterior approach is less traumatic than the combined posterior-anterior approach, but with a much slower lesion healing and activity recovery.
Prognostic value of Glypican family genes in early-stage pancreatic ductal adenocarcinoma after pancreaticoduodenectomy and possible mechanisms
Authors: Liu Jun-qi , Liao Xi-wen , Wang Xiang-kun , Yang Cheng-kun , Zhou Xin , Liu Zheng-qian , Han Quan-fa , Fu Tian-hao , Zhu Guang-zhi , Han Chuang-ye , Su Hao , Huang Jian-lu , Ruan Guo-tian , Yan Ling , Ye Xin-ping , Peng Tao 
Published: August 2020

Abstract
Background: This study explored the prognostic significance of Glypican (GPC) family genes in patients with pancreatic ductal adenocarcinoma (PDAC) after pancreaticoduodenectomy using data from The Cancer Genome Atlas (TCGA).Methods: A total of 112 PDAC patients from TCGA were included in the analysis.The relationship between overall survival and the expression of GPC family genes as well as basic clinical characteristics was analyzed using the Kaplan-Meier method with the log-rank test. Joint effects survival analysis was performed to further examine the relationship between GPC genes and prognosis. A prognosis nomogram was established based on clinical characteristics and prognosis-related genes. Prognosis-related genes were investigated by genome-wide co-expression analysis and gene set enrichment analysis (GSEA) to identify potential underlying mechanisms.Results: High expression of GPC2, GPC3, and GPC5 was significantly associated with favorable survival (log-rank P = 0.031, 0.021, and 0.028, respectively; adjusted P value = 0.005, 0.022, and 0.020, respectively), and joint effects analysis of these genes was effective for prognosis prediction. The prognosis nomogram was applied to predict the survival probability using the total score calculated. Genome-wide co-expression and GSEA analyses suggested that the GPC2 mechanisms affecting prognosis involved sequence-specific DNA binding, protein transport, cell differentiation and oncogenic signatures (KRAS, RAF, STK33, and VEGFA). GPC3 may be related to cell adhesion, angiogenesis, inflammatory response, signaling pathways like Ras, Rap1, PI3K-Akt, chemokine, GPCR, and signatures like cyclin D1, p53, PTEN. GPC5 may be involved in transcription factor complex, TFRC1, oncogenic signatures (HOXA9 and BMI1), gene methylation, phospholipid metabolic process, glycerophospholipid metabolism, cell cycle, and EGFR pathway.Conclusion: GPC2, GPC3, and GPC5 expression may serve as prognostic indicators in PDAC, and the combination of these genes showed a higher efficiency for prognosis prediction.
Ozone Induces Autophagy by Activating PPARγ/mTOR in Rat Chondrocytes Treated with IL-1β
Authors: Sun panpan , Xu Weichneg , Zhang Cong , Zhao Xu , Lin Xiaowen , Gong Moxuan , Fu Zhijian 
Published: August 2020

Abstract
Background: Osteoarthritis (OA) is the main cause of older pain and disability, its dysfunction due to discomfort as well as the quality of life of patients having adverse effects. Medical ozone (O3) has been found to have antioxidative and anti-inflammatory effects in the treatment of osteoarthritis. However, it is unclear whether O3 can induces autophagy through the PPARγ/mTOR autophagy pathway in chondrocytes treated with IL-1β. Methods: Primary chondrocytes were isolated from wistar rat cartilage within 3days. The OA chondrocyte model was induced via treatment with IL-1β to chondrocytes for 24 hours. Then the cells were treated with O3 and GW9662, the inhibitor of PPARγ. Cell viability was assessed by CCK-8. Further, the cells subjected to western blot analysis, qRT-PCR and immunofluorescence assay. The numbers of autophagosomes were observed via transmission electron microscopy. Results: 30μg/ml O3 improved the viability of chondrocytes. O3 significantly increased the level of autophagy proteins and the numbers of autophagosomes in chondrocytes treated with IL-1β via treated with O3. qRT-PCR results showed that O3 decreased the levels of IL-6,TNF-α and MMP-3,MMP-13 in chondrocytes treated with IL-1β. Conclusions: 30μg/ml O3 improved autophagy via activating PPARγ/mTOR signaling and suppressing inflammation in chondrocytes treated with IL-1β.