bluuebunny/crossref_metadata_2025_split huggingface dataset:
Long term evaluation of corneal graft survival after lamellar Keratoplasty without limbal stem cell transplantation in patients with delayed-onset mustard gas keratopathy.
Authors: Bagheri Shiva , Daryabari Seyed-Hashem , Aghamollaei Hossein , Jadidi Khosrow 
Published: November 2020

Abstract
Background: Iran is one of the biggest chemical warfare victims in the world and mustard gas keratopathy is a common complication in this rare injury. in recent years, finding an effective and uncomplicated corneal transplantation method has been a concern for ophthalmologists. In this study, we report the results of lamellar Keratoplasty (LK) without limbal stem cell transplantation in patients with delayed-onset mustard gas keratopathy (DMGK). Methods: Thirty-one eyes of 22 veterans with DMGK who underwent conventional LK were included. The presence of limbal stem cell deficiency (LSCD) was proven clinically and so by impression cytology. The results were evaluated with respect to Best spectacle-corrected visual acuity (BSCVA), refractive error (RE), corneal clarity and corneal graft survival rates.Results: The mean age of patients at the time of surgery was 52.93±5.8 years and the mean follow-up duration was 36.25±20.65 months. The mean preoperative BSCVA was 1.67±0.32 logarithm of the minimal angle of resolution unit (Log MAR), which significantly improved and reached 0.32±0.22 Log MAR after surgery. The mean preoperative spherical equivalent RE was 2.47±1.17 diopters which increased to 3.5±1.08 diopters after complete suture removal. Epithelial graft rejection occurred in 9.67% of eyes (3/31) and was treated successfully with topical eye drops. at the end of follow-up period, no signs of graft rejection were found but two eyes had mild central corneal opacity. Conclusions: According to the results of this study, LK without LSC transplantation, can be successful in patients with DMGK, because of partial rather than complete LSCD.
Imaging of stem cell therapy in Acute Respiratory Distress Syndrome in sheep and its relationship with histopathology
Authors: Chaleshtori Sirous Sadeghian , Dezfouli Mohammad Reza Mokhber , Abbasi Javad , Fakhr Massoumeh Jabbari , Vajhi Alireza , Esmaeilnejad Mohammad Reza , Mohajeri Saeed Farzad 
Published: November 2020

Abstract
Methods: 10 healthy sheep were separated as the control (PBS) and treatment(BM-MSCs). The BM-samples were aspirated in the treatment group and isolation and expansion of BM-MSCs were achieved. 24h after ARDS-induction by E.coli LPS (400 µg/kg), 5×107 BM-MSCs and 1 ml PBS were intrapulmonary infused in the treatment and control groups, respectively. The total lung volume and the Hounsfield unit by CT-scan and the cardiac parameters by echocardiography were calculated before of ARDS (time -24) and cells/PBS infusion time(time 0) and then for 6,12,24,48,72,168h after cells/PBS infusion. At the end, the sheep were sacrificed and the hearts and lungs were macroscopically and microscopically checked.Results: The results showed the total lung volume and the Hounsfield unit increased at time 0, but BM-MSCs declined their amount, so that the changes were significant at 168h compared with time 0 and comparisons between the two-groups represented the significant difference at 72h and 168h. The cardiac parameters did not changes significantly in the two-groups at different times but the comparison between them demonstrated significant difference in LVPWs, IVSs,IVSd,%FS,RVOTVmax,LA(d1) and AO(d2) at 168 h, LVIDs,ESV and %EF at 72h and 168h and LVPWd at 72h. Also, the histopathology findings indicated decline of inflammatory reactions, edema, hemorrhage and hyperemia in the respiratory system and no observation of damage to the heart compared with the control-group. Conclusions: The results determined BM-MSCs in ARDS decrease edema and inflammation of the lungs and increase the alveoli air volume and prevent damage to heart function.
Headache in the emergency department in Chongqing Province, China: A multicentre study
Authors: Li Qin , Ye Tao , Ma Jingxi , Yuan Dongli , Peng Xuemei , Cai Min , Xi Zhengwei , Zhang Yujiao , Liu Qinlin , Tan Ge 
Published: November 2020

Abstract
Background: To investigate the present situation of emergency headache in many general hospitals in Chongqing Province, China.Methods: From January 1 to December 31, 2017, we studied patients who complained of headaches as the main symptom in the emergency department of 17 general hospitals in Chongqing Province. Each patient with a headache was interviewed and the medical history was collected by administering a questionnaire. The diagnosis of headache is based on the third edition of the International Classification of Headache Disorders (ICHD-3 beta). Results: In 2017, 16434 patients complained of headache in 17 general hospitals in Chongqing, accounting for 2.3% of all emergency patients. Eight thousand one male patients (48.7%) and 8433 female patients (51.3%) experienced a headache. The average age was (41.8 ± 13.2) years, and the course of disease was 3 hours to 15.2 years. Regarding the initial diagnosis, 7299 patients (44.4%) were diagnosed secondary headache, 5076 (30.9%) were diagnosed with primary headache and 4059 (24.865%) could not be diagnosed. The hospitalization rates for these patients were 60.3%, 12.2%, and 41.5%, respectively. Migraine is still the most common type of primary headache. Conclusions: This study shows that the misdiagnosis rate and missed diagnosis rate of patients with an emergency headache are high, and the emergency department still performs overtesting and hospitalizations.
Implementation and Sustainability of an Enhanced Pulmonary Rehabilitation Program in a single centre
Authors: Braak Kim van der , Wald Joshua , Tansey Catherine , Paes Thais , Sedeno Maria , Selzler Anne-Marie , Stickland Michael , Bourbeau Jean , Janaudis-Ferreira Tania 
Published: November 2020

Abstract
Background: Pulmonary rehabilitation (PR) has major benefits for patients with chronic obstructive pulmonary disease (COPD). The availability of PR in Canada is low. To facilitate implementation, a standardized and enhanced PR Program has been developed with a self-management education intervention that has been validated and shown to be effective. The objectives of our study were to assess the implementation of this program into a single site; determine the sustainability 18 months after implementation; and to identify the satisfaction with, facilitators of and barriers to implementation and sustainability of the program as perceived by patients with COPD and HCPs. Methods: We conducted a prospective pre-post study which consisted of two phases: 1) the implementation phase (first six months after implementation) and 2) the sustainability phase (18 months after implementation). Guided by the RE-AIM framework, outcomes including: Reach (number of patients enrolled and demographics), Effectiveness (Change in patient outcomes), Adoption (HCPs’ characteristics), Implementation (Program fidelity) and Maintenance (Patient outcomes at 3-month follow-up). Results: Reach: Twenty-six patients were included for both phases (two different samples). Effectiveness: Clinically important improvements in patient outcomes were found for functional exercise capacity, knowledge, functional status and self-efficacy in both phases of the study. Adoption: All HCPs involved in PR (N=8) agreed to participate and used the program in both phases of the study. Implementation: Fidelity for the group education sessions ranged from 76-95% in the implementation phase and from 82-88% in the sustainability phase. Maintenance (implementation phase): Clinical important improvements at 3-month follow-up were found in knowledge, self-efficacy for exercise and walking. Patients and HCPs were highly satisfied with the program. Lack of time was reported as the most common barrier and having longstanding procedures as the most common facilitator by HCPs to implementation and sustainability of the program. Conclusions: The enhanced PR program was accepted by the patients and HCPs and could be reliably implemented and maintained at a single expert center. The program provided clinical benefits for patients in terms of functional capacity, knowledge and self-efficacy for walking. These findings will guide planning for wide scale dissemination and implementation of the program.
Anti-echinococcal effect of verapamil involving regulating calcium/calmodulin-dependent protein kinase Ⅱ response in vitro and in a murine infection model
Authors: Gao Hai-Jun , Sun Xu-Dong , Luo Yan-Ping , Pang Hua-Sheng , Ma Xing-Ming , Zhang Ting , Jing Tao , Hu Wei , Shen Yu-Juan , Cao Jian-Ping 
Published: November 2020

Abstract
Background: Echinococcosis caused by the larval stage of cestode of the genus Echinococcus is a parasitic zoonosis, imposing serious threat on the health of humans and animals globally. Albendazole is the drug of choice for treatment of echinococcosis, but it is difficult to meet the clinical challenge in chemotherapy due to its low curative rate and severe side effects. Hence, novel anti-parasitic targets and effective treatment alternatives are urgently needed. Previous study has showed that verapamil can suppress the growth of Echinococcus granulosus (E. granulosus) larva, but the mechanism remains unclear. The aim of the present study is to gain insight into the anti-echinococcal effect of verapamil on Echinococcus with particular focus on the regulatory role of verapamil to calcium/calmodulin-dependent protein kinase Ⅱ (Ca2+/CaM-CamK Ⅱ) in infected mice.Methods: The anti-echinococcal effect of verapamil on E. granulosus protoscolex (PSCx) in vitro and Echinococcus multilocularis (E. multilocularis) metacestodes in infected mice were assessed. The morphological alteration of Echinococcus spp. induced by verapamil was observed by scanning electron microscope (SEM), and the changes of calcium content in both parasite and mice sera and livers were measured by SEM-energy dispersive spectrometer, inductively coupled plasma mass spectrometry and alizarin red staining accordingly. Additionally, the changes on the protein and mRNA levels of CaM and CamK Ⅱ in infected mice, and on the mRNA levels of CamK Ⅱ in E. granulosus PSCx after treatment of verapamil were evaluated by immunohistochemistry or/and real-time quantitative polymerase chain reaction (RT-qPCR).Results: In vitro, E. granulosus PSCx could be killed by verapamil at 0.5 μg/mL or more within 8 days. Under these conditions, the ultrastructure of PSCx was damaged and accompanied with obvious calcium loss and down-regulation of CamK Ⅱ mRNA. In vivo, the weight and the calcium content of E. multilocularis metacestodes from the mice were reduced after treatment with verapamil at 40 mg/kg, meanwhile, an elevation of calcium content in the serum and liver of infected mice was observed. In addition, down-regulation of both the over-expressed CaM and CamK Ⅱ proteins and mRNAs in the liver of mice infected with E. multilocularis metacestodes were found after treatment of verapamil.Conclusions: Verapamil exerted a parasiticidal activity on Echinococcus both in vitro and in vivo through down-regulation of Ca2+/CaM-CamK Ⅱ that was over-activated by parasitic infection. It has been speculated that the Ca2+/CaM-CamK Ⅱ can be a novel drug-target and verapamil is shown to be a potential anti-echinococcal drug for controlling echinococcosis in the future.
Satisfaction with nursing care and its related factors in patient with COVID-19
Authors: Parizad Naser , Goli Rasoul , Arad Mansour , Baghaie Rahim , Habibzadeh Hossein 
Published: November 2020

Abstract
Background: Patients’ satisfaction is a fundamental factor in the quality of nursing care. The emergence of the novel coronavirus (COVID-19) and the highly contagious virus can affect nursing care by increasing the number of care-seekers. This study aimed to determine the patients’ satisfaction and related factors in patients with COVID-19 hospitalized in Taleghani Hospital, Urmia-Iran, in 2020.Methods: This descriptive correlational study was conducted on 196 patients with COVID-19 hospitalized in Taleghani Hospital, Urmia. Purposive convenient sampling was used to recruit participants. Study participants completed Patient Satisfaction Instrument (PSI) and demographics questionnaires. Data were analyzed with SPSS software version 25.0.Results: The majority of patients (68.9%) were moderately satisfied with nursing care. Based on Pearson Correlation Analysis, only residential status had a significant inverse relationship with satisfaction level in patients with COVID-19 (r = -0.0238, P=0.001).Conclusion: The patients’ satisfaction with nursing care was mostly at a moderate level. Thus, there is a need to educate health personnel and nurses in particular and motivate them to have active participation in achieving patients’ satisfaction with COVID-19. Notably, only the institutes that take patients’ satisfaction as a top priority can succeed in a competitive market of health services.
Subjective experience of time in dementia with Lewy bodies during COVID-19 lockdown
Authors: Torboli Dylan , Mioni Giovanna , Bussé Cinzia , Cagnin Annachiara , Vallesi Antonino 
Published: November 2020

Abstract
Dementia with Lewy bodies (DLB) is a neurodegenerative disease characterized by cognitive, behavioral and motor symptoms and has a more challenging clinical management and poorer prognosis respect to other forms of dementia. The experience of lockdown leads to negative psychological outcomes for fragile people such as elderly with dementia, particularly for DLB, causing a worsening of cognitive and neuropsychiatric symptoms. An individual’s feeling of time passage is strongly related to her cognitive and emotional state. We therefore assessed the subjective experience of the passage of time for present and past time intervals (Subjective Time Questionnaire, STQ) during the lockdown due to coronavirus disease (COVID-19) in 23 patients with DLB (18 of which were re-tested in a post-lockdown period) and compared their experience with that of 21 caregivers with similar age and 46 younger university students. Patients showed a significantly slower perception of time and also reported feeling less time pressure. We argue that these alterations might be related to the distinctive features of DLB and their exacerbation recorded by the patients’ caregivers during the period of lockdown, though our results show that the patients’ experience of time passage in a post-lockdown period remained similarly slow. Overall, we show an impairment of the subjective perception of time passage in DLB tested during the COVID-19 lockdown.
Predictive model for COVID 19 curve - An evolutionary approach
Authors: Rangarajan Srikanth , Poranki Srikanth , Sammakia Bahgat 
Published: November 2020

Abstract
In this manuscript we propose a novel method that models the evolution, spread and transmission of COVID 19 pandemic. The proposed model is inspired partly from the evolutionary based state of the art genetic algorithm. The rate of virus evolution, spread and transmission of the COVID 19 and its associated recovery and death rate are modeled using the principle inspired from evolutionary algorithm. Furthermore, the interaction within a community and interaction outside the community is modeled. Using this model, the maximum healthcare threshold is fixed as a constraint. Our evolutionary based model distinguishes between individuals in the population depending on the severity of their symptoms/infection based on the fitness value of the individuals. There is a need to differentiate between virus infected diagnosed (Self isolated) and virus infected non-diagnosed (Highly interacting) sub populations/group. In this study the model results does not compare the number outcomes with any actual real time data based curves. However, the results from the model demonstrates that a strict lockdown, social-distancing measures in conjunction with more number of testing and contact tracing is required to flatten the ongoing COVID-19 pandemic curve. A reproductive number of 2.4 during the initial spread of virus is predicted from the model for the randomly considered population. The proposed model has the potential to be further fine-tuned and matched accurately against real time data.
Apolipoprotein C1 stimulates the malignant process of renal cell carcinoma via the Wnt3a signaling&nbsp;
Authors: jiang hao , Tang Jing-Yuan , Xue Dong , Chen Yi-Meng , Wu Ting-Chun , Zhuang Qian-Feng , He Xiao-Zhou 
Published: November 2020

Abstract
Background Renal cell carcinoma (RCC) is a clinically common tumor in the urinary system, showing an upward trend of both incidence and mortality. Apolipoprotein C1 (APOC1) has been identified as a vital regulator in tumor progression. This study aims to uncover the biological function of APOC1 in RCC process and the underlying mechanism. MethodsDifferential levels of APOC1 in RCC samples and normal tissues in a downloaded TCGA profile and clinical samples collected in our center were detected by quantitative reverse transcription PCR (qRT-PCR). The prognostic value of APOC1 in RCC was assessed by depicting Kaplan-Meier survival curves. After intervening APOC1 level by transfection of sh-APOC1 or oe-APOC1, changes in phenotypes of RCC cells were examined through CCK-8, colony formation, Transwell assay and flow cytometry. Subsequently, protein levels of EMT-related genes influenced by APOC1 were determined by Western blot. The involvement of the Wnt3a signaling in APOC1-regulated malignant process of RCC was then examined through a series of rescue experiments. Finally, a RCC xenograft model was generated in nude mice , aiming to further clarify the in vivo function of APOC1 in RCC process.ResultsAPOC1 was upregulated in RCC samples. Notably, its level was correlated to overall survival of RCC patients, displaying a certain prognostic value. APOC1 was able to stimulate proliferative, migratory and invasive abilities in RCC cells. The Wnt3a signaling was identified to be involved in APOC1-mediated RCC process. Notably, Wnt3a was able to reverse the regulatory effects of APOC1 on RCC cell phenotypes. In vivo knockdown of APOC1 in xenografted nude mice slowed down the growth of RCC.ConclusionsAPOC1 stimulates the malignant process of RCC via targeting the Wnt3a signaling.
Gender differences in self-harm and drinking behaviors among high school students in Beijing, China
Authors: Li Chai-Quan , ZHANG Jing-Shu , MA Shang , Ruo-Ran LV , DUAN Jia-Li , LUO Dong-Mei , YAN Xiao-Jin , MA Ning , SONG Yi 
Published: November 2020

Abstract
BackgroundSelf-harm and drinking were both serious problems in adolescents and many studies presented evidence of their association. However, gender differences in this association was seldom deeply discussed. Our study was aimed to evaluate the prevalence of self-harm and explore its association with drinking behaviors by gender and investigate the extent to which the gender differences exist in the association between self-harm and drinking.MethodsA total of 32,362 students in grades 7 to 12 were anonymously surveyed and included in our study using two-stage, stratified probability proportion sampling in Beijing, China. Self-harm, drinking behaviors and other basic information were obtained from anonymous questionnaire. Demographic variables, self-harm as well as drinking behaviors were analyzed with Chi-square test and Gamma test between genders and the gender differences in this association was analyzed by log-binomial regression. ResultsThe total prevalence of self-harm was 13.7% with no significant gender difference (B=0.352, P=0.553). The prevalence of self-harm decreased with age in girls (G=-0.163, P<0.001). Self-harm was associated with drinking behaviors in both boys and girls. The Log-binomial regression demonstrated that girls in 16-19 years were at lower risk of self-harm than girls in 12-15 years while this association was weaker in boys (1.493 vs 1.128). The higher OR for self-harm was found among girls with early drinking experiences compared with boys (2.565 vs 1.863). Girls who ever drunk (2.211 vs 1.636), were currently drinking (3.400 vs 2.122) and binged drinking (6.357 vs 3.924) were of greater risk for self-harm than boys. ConclusionSelf-harm has significant positive association with drinking among high school students and girls with drinking behaviors were at higher risk of suffering self-harm. Identifying adolescents’ drinking behaviors is of vital importance to self-harm prevention and special attention should be focused on younger girls.
Cost-utility analysis of pralatrexate for relapsed or refractory peripheral T-cell lymphoma based on a case-matched historical control study along with single arm clinical trial
Authors: Park Seonyoung , Kim Ah-Young , Cho Hyeonseok , Baik Deborah , Lee Hankil , Cho Sunghwa , Kang hye-young 
Published: November 2020

Abstract
Background: Patients with relapsed or refractory peripheral T-cell lymphoma (R/R PTCL) treated with pralatrexate have previously shown superior overall survival (OS) compared to those who underwent conventional chemotherapy (CC, 15.4 vs. 4.07 months). We conducted an economic evaluation of pralatrexate from a societal perspective in Korea based on data from the PROPEL phase II study.Methods: Using a Markov model with a weekly cycle, we simulated the experience of patients with R/R PTCL receiving pralatrexate or CC for 15 years. The model consists of five health states; initial treatment, treatment pause, subsequent treatment, stem cell transplantation (SCT) success, and death. Comparative effectiveness was based on PROPEL phase II single-arm study and its matched historical control analysis. Costs included drug, drug administration, monitoring, adverse event management, and SCT costs. Results: The incremental cost-effectiveness ratio of the base case was $39,153 per quality-adjusted life-year (QALY) gained. The results of one-way sensitivity analysis ranged from $33,949 to $51,846 per QALY gained, which remained within an implicit willingness-to-pay (WTP) threshold of anticancer drugs in Korea. Conclusions: Pralatrexate is a cost-effective intervention with improved OS and incremental costs within the WTP limit. Pralatrexate could function as a new therapeutic option for patients suffering from life-threatening R/R PTCL.