bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association of Periodontal Diseases with Intracranial Aneurysm Formation: Novel Predictive Indicators
Authors: Liu Keyun , Sun Jia , Shao Lingling , He Hongwei , Liu Qinglin , Li Youxiang , Ge Huijian 
Published: July 2020

Abstract
Background and purpose: We investigated whether periodontal diseases, specifically, periodontitis and gingivitis, could be risk factors of the incidence of intracranial aneurysms (IAs).Methods: We performed a case–control study to compare the differences in the periodontal disease parameters of 281 cases that were divided into the IA and non-IA groups. All cases underwent complete radiographic examination for IAs and examination for periodontal health. Results: Compared with those in the non-IA group, the cases in the IA group were older (53.95 ± 8.56 vs 47.79 ± 12.33, p < 0.001) and had a higher incidence of hypertension (76 vs 34, p = 0.006). Univariate analysis revealed that age (>50 years) and hypertension were risk factors of aneurysm formation (odds ratio [OR] 1.047, 95% confidence interval [95%CI] 1.022–1.073, p < 0.001 and OR 2.047, 95%CI 1.232–3.401, p = 0.006). In addition, univariate and multivariate logistic regression analyses showed that the parameters of periodontal diseases, including gingival index, plaque index, clinical attachment loss, and alveolar bone loss, were significantly associated with the occurrence of IA (all p < 0.05). For further statistical investigation, the parameters of periodontal diseases were divided into four layers on the basis of their averages. Poor periodontal health condition was correlated with a high risk of IAs. Conclusion: Periodontal diseases, especially severe gingivitis or periodontitis combined with hypertension, were significantly associated with the incidence of IAs.
Virtual Patients and Clinical Decision-making Skills: How Students Learn to Diagnose Depending on Their Psychological Characteristics
Authors: Zalewski Bartosz , Walkiewicz Maciej , Guziak Mateusz 
Published: July 2020

Abstract
Background:The goal of this exploratory study is to analyse which psychological characteristics of clinical psychology students are related to the effectiveness of learning clinical decision-making skills with the use of virtual patient (VP).Methods: Authors’ VP was used for measuring the ability to learn the decision-making: negative vs positive aspects of a patient’s functioning, reactance, coping, stage of change, cognitive errors, adequacy of assessment data, and the quality of assessment. Psychological questionnaires were used for measuring student’s: need for cognitive closure; ability to achieve closure; beliefs of changeability on human traits; the level of hope, intelligence, positive vs negative affect, and academic knowledge. Results: Developing clinical decision-making with the use of VP is effective for students who have a higher ability to tolerate ambiguity and complexity of data. Students convinced of the changeability of human traits take into consideration more diverse data and the process of diagnosis requires the analysis of complex phenomena. Students with dominant negative affect seem to build diagnoses more carefully and make fewer cognitive errors than those with positive affect.Conclusions:Our study indicates which properties of participants support and which weaken the acquisition of the ability to make a diagnosis.
Death by SARS-CoV 2 - a Romanian COVID-19 multi-centre comorbidity study
Authors: Stoian Anca Pantea , Pricop-Jeckstadt Mihaela , Pana Adrian , Ileanu Bogdan-Vasile , Schitea Ruxandra , Geanta Marius , Catrinoiu Doina , Suceveanu Andra Iulia , Serafinceanu Cristian , Pituru Silviu , Poiana Catalina , Timar Bogdan , Nitipir Cornelia , Parvu Simona , Arsene Andreea , Mazilu Laura , Toma Antonela , Hainarosie Razvan , Ceriello Antonio , Rizzo Manfredi , Jinga Viorel 
Published: July 2020

Abstract
Evidence regarding the relation between SARS-CoV-2 mortality and the underlying medical condition is scarce. We conducted an observational, retrospective study based on Romanian official data about location, age, sex and comorbidities for COVID-19 fatalities. Our findings indicate that males, hypertension, diabetes, obesity and chronic kidney disease were most frequent in the COVID-19 fatalities, that the burden of disease was low, and that the prognosis for 1-year survival probability was high in the sample. Evidence shows that age-dependent pairs of comorbidities could be a negative prognosis factor for the severity of disease for the SARS-CoV 2 infection.
The effectiveness of implementation of mandatary institutional isolation of all mild cases by developing Fangcang hospitals against the Corona Virus Disease 2019 (COVID-19): modified SEIR model simulation
Authors: Zeng Zhechun , WANG Jinwen , SU Jianglian , ZUO Huijuan , Xie Jiang 
Published: July 2020

Abstract
Background

An epidemic outbreak of a novel coronavirus (COVID-19) pneumonia has occurred in Wuhan City, China in December 2019 and subsequently led to the breakdown of the medical system. Fangcang hospital is developed to isolate patients with mild symptoms of an infectious disease from their families and communities on 28 January 2020. We present use a mathematical model to quantify the impact of the mandatory isolation strategy on the epidemic in Wuhan.
Methods

We developed a modified Susceptible-Exposed-Infectious-Removed (SEIR) model to simulate the Wuhan COVID-19 outbreak. We use reported case data up to 1 December 2019 from the Chinese Center for Disease Control and Prevention to parameterize the model. We used the model to estimate reductions in the number of confirmed cases and deaths in Wuhan.
Results

Compared with mandatory institutional isolation measures, home isolation for mild cases increase the number of confirmed cases from 51,214 to 61,349 in Wuhan, but has a more marked effect at the mortality, where death case increased by nearly 35.3% until April 25th.
Conclusion

The mandatory isolation strategy by using Fangcang hospitals applied on January 28, 2020 can effectively control the outbreak of COVID-19. We suggest that a mandatory isolation strategy be applied with success around the whole world.
Ozone Pollutants and the Onset of COVID-19 Symptoms in Chiba Prefecture, Japan
Authors: Dao Hang Viet , Fanjul Katrina , LE Ngoan Tran 
Published: July 2020

Abstract
Background

We examined the association between ozone pollutants in the breathing air and the onset of symptoms of COVID-19 infection.
Methods

We accessed online data of positive COVID-19 cases published by the Chiba Prefectural Government, including their ID, residence, age, sex, the dates of onset of symptoms, and tested positive with COVID-19. The daily data ozone concentrations were derived from an online database of 20 weather stations in the prefecture. The data of daily onset of symptoms of 735 patients were linked with the daily data of ozone concentrations. We estimated the number of daily person-years by dividing the number of the population by 365 (days). The incidence rate ratio and 95% confidence interval (IRR, 95% CI) were estimated by the levels of ozone concentrations.
Results

There was a significant positive association between the date of symptom onset and levels of ozone concentrations. Adjusted IRR, 95% CI, tertile_3 vs. tertile_1, was 1.69 (1.26, 2.26) for total participants, p for trend = 0.000. The positive association was observed in women but not in men, adjusted IRR, 95% CI, tertile_3 vs. tertile_1: 3.18 (1.94, 5.22), and 1.07 (0.73, 1.57), respectively. The positive association was seen for the sub-groups of ages under 60 but not 60+, adjusted IRR, 95% CI, tertile_3 vs. tertile_1: 1.90 (1.37, 2.65) and 1.10 (0.57, 2.17), respectively.
Conclusions

Ozone pollutants affect susceptibility to COVID-19 in the onset of symptoms. The findings warrant further studies on the mechanism of how ozone pollutants affect susceptibility to COVID-19.
P300-dependent acetylation of histone H3 is required for EGFR-mediated HMGA2 transcription in hepatocellular carcinoma
Authors: Liang Chao , Niu Jie , Wang Xiao , Yao Xin , Yang Ruo-Han , Zhang Zhan-Sheng , Liu Fan-Ye , Li Wen-Qi , Pei Shu-Hua , Sun Hua , Wang Chao-Jie , fang dong , Xie Song-Qiang 
Published: July 2020

Abstract
Background

High-mobility group protein A2 (HMGA2) is highly expressed in hepatocellular carcinoma cells and contributes to tumor metastasis and poor patient survival. However, the molecular mechanism of HMGA2 transcriptional regulation in hepatocellular carcinoma cells remains largely unclear.
Methods

Mouse model of lung metastasis was used to evaluate the effects of HMGA2 on hepatocellular carcinoma. DEN-induced rat model of hepatocellular carcinoma was used to measure the expression of HMGA2 and histone H3 acetylation. Immunohistochemistry was used to analyze the expression levels of HMGA2 in 39 matched HCC tissues and adjacent non-tumor tissues. Real-time PCR, Western blotting and Chromatin Immunoprecipitation assay were used to investigate the underlying mechanisms by which HMGA2 overexpression in hepatocellular carcinoma cells.
Result

Compared with adjacent non-tumour tissues, the expression of HMGA2 was significantly upregulated in human HCC tumor tissues. Moreover, We demonstrated that the expression HMGA2 was upregulated in HCC, and the elevated HMGA2 could promote tumor metastasis. Incubation HCC cells with Epidermal growth factor (EGF) could promote the expression of HMGA2 mRNA and protein. Knockdown of p300 can reverse EGF-induced HMGA2 expression and histone H3-K9 acetylation, while a phosphorylation-mimic p300 S1834D mutant can stimulate HMGA2 expression as well as H3-K9 acetylation in HCC cells. Furthermore, we identified p300-mediated H3-K9 acetylation participates in EGF-induced HMGA2 expression in HCC. In addition, the levels of H3-K9 acetylation positively correlated with the expression levels of HMGA2 in human HCC specimens.
Conclusion

p300-dependent acetylation of histone H3 is required for EGFR-mediated HMGA2 transcription in hepatocellular carcinoma.
Follow up the children with ocular lesion induced by two common intrauterine infections (Cytomegalovirus,Toxoplasmosis): A cohort study in Tehran, Iran
Authors: Vafaee-Shahi Mohammad , noorbakhsh samileh , Tahernia Leila , Ashouri Sarvenaz 
Published: July 2020

Abstract
Background

Intrauterine infections lead to involvement of various organs in fetus, including the eye. The aim of this study was to determine the frequency and clinical response of ocular lesions to specific drugs in infants with congenital Toxoplasma and cytomegalovirus infections.
Methods

This historical cohort study was performed in the pediatric and ophthalmology department of Hazrat Rasool Akram Hospital in Tehran from October 2011 to November 2017. Patients included 78 infants with intrauterine infection (proven) and ocular involvement (cataracts, glaucoma, and retinitis). Infants who did not undergo additional and diagnostic tests, eye examinations, follow-up and did not receive effective treatment were excluded from the study. Three patients expired during the study. Finally, 37 patients (including 25 patients with cytomegalovirus and 12 patients with toxoplasma) were included to assess and the clinical response to ocular lesions was evaluated for one year.
Results

Of 12 cases with toxoplasmosis, 5 patients received complete treatment and 4 patients had appropriate clinical responses. Of 25 patients with CMV, 18 patients received complete treatment and 9 patients had appropriate clinical responses.
Conclusion

Successful treatment will be obtained in near 80% of ocular toxoplasmosis,so anti toxoplasma treatment is recommended in all confirmed cases .About 50% of CMV infected cases (with hearing loss, ocular involvement ) might respond well to antiviral therapy, We recommend anti CMV treatment in any age (< 2 years old) but it is not indicated in CMV infected cases with severe brain involvement.
Rapid Skin Temperature Measurement is Valuable in Evaluating Inflammation and Functional Status After Total Knee Arthroplasty：A Case Control Study
Authors: Xu Hao , Huang Hui , Yin Lu-xu , Xiang Shuai , Shang Guang-qian , Zhang Hai-ning , Wang Ying-zhen , Yan Weining 
Published: July 2020

Abstract
Background：The skin temperature of the knee joint changes after total knee arthroplasty (TKA), and these changes are easy to detect and can be measured simply and accurately, but its significance is not clear.Methods: A total of 205 patients who underwent unilateral primary TKA were selected; the bilateral knee skin temperature, inflammatory indicators, pain score, knee range of motion (ROM) and quadriceps muscle strength of the patients were measured from preoperatively to 6 months postoperatively; and the relationships between the differential skin temperature and inflammatory and functional prognosis were analyzed.Results: The differential skin temperature was significantly correlated with the inflammatory index, pain score, ROM and muscle strength. A difference in skin temperature between the two sides of >0.52°C at 2 weeks after the operation suggested that the level of c-reactive protein increased (sensitivity 81.4%, specificity 39.8%) and affected the ROM at 2 weeks and 6 weeks after the operation, as well as the muscle strength at 6 weeks and 3 months after the operation. A difference in skin temperature of >2.15°C at 2 weeks after the operation suggested that the level of c-reactive protein increased by more than three times (sensitivity 90.9%, specificity 95.2%) and affected the ROM at 3 months and 6 months after the operation and muscle strength at 6 weeks and 3 months after the operation.Conclusion: The difference in the knee skin temperature between the operated side and contralateral side is closely related to functional recovery and the magnitude of increase in inflammatory indicators in patients after TKA. Skin temperature measurements can be used as references for predicting functional recovery and inflammatory indicators.Trial registration：Medical Ethics Committee of Affiliated Hospital of Qingdao University,QYFYKYLL2327945,2015-03-01
IVIM Magnetic Resonance Imaging in Guiding Target Delineation and Dose Escalation of Concurrent Radiochemotherapy in Recurrent Stage IIIB Pulmonary Adenosquamous Carcinoma: A Case Report
Authors: Xu Peng , Chen Yixuan , Liu Anwen , Cai Jing 
Published: July 2020

Abstract
Background: Concurrent radiochemotherapy is regarded as a standard treatment for inoperable, locally advanced non-small cell lung cancer (NSCLC). However, the local control rate is less than 50%. Functional imaging can reveal changes in the tumour microstructure and tumour volume early during the process of radiotherapy, which is beneficial for the early evaluation of efficacy and dose escalation radiotherapy.Case presentation: Here, we describe a case of recurrent stage IIIB pulmonary adenosquamous carcinoma. Intravoxel incoherent motion (IVIM) was used to guide the delineation of the target area and dose escalation of radical concurrent chemoradiotherapy, and complete remission was achieved.Conclusion: Our case report provides a novel method for IVIM functional MRI in guiding accurate target delineation and dose escalation of radical radiotherapy.
Reflux Esophagitis and Fatigue: Are They Related?
Authors: Kang Sung-Goo , Hwang Hyun jee , Kim Youngwoo , Lee Junseak , Oh Jung Hwan , Kim Jinsu , Lim Chul-Hyun , Youn Seung Bae , Jung Sung Hoon 
Published: July 2020

Abstract

Background: Gastroesophageal reflux disease (GERD) is a chronic, recurrent disease. Reflux esophagitis can interfere with sleep via acid reflux, which could cause daytime sleepiness or fatigue. However, little is known about the association between reflux esophagitis and fatigueObjectives: We evaluated the association between fatigue and reflux esophagitis in subjects seen at health check-ups.Methods: Consecutive patients who were scheduled for screening endoscopy were enrolled prospectively at the Comprehensive Medical Examination Center of St. Vincent Hospital and Eunpyeong St. Mary's Hospital, Korea. Three validated questionnaires were used to assess fatigue, daytime hypersomnolence, anxiety, and depression: the Multidimensional Fatigue Inventory-Korean version (MFI-K), Epworth Sleepiness Scale (ESS), and Hospital Anxiety and Depression Scale (HADS).Results: We investigated 497 consecutive eligible subjects. The reflux esophagitis and symptomatic GERD groups comprised 103 (20.7%) and 92 (18.5%) subjects, respectively. The MFI-K total, ESS, HADS-anxiety, and HADS-depression scores did not differ between the esophagitis and non-esophagitis groups (50.0±11.5 vs. 49.7±10.9, P=0.769; 6.2±2.8 vs. 6.1±3.1, P=0.819; 5.8±3.1 vs. 5.2±3.2, P=0.069; 6.2±3.6 vs. 6.0±3.3, P=0.527). However, the MFI-K total, ESS, HADS-anxiety, and HADS-depression scores were higher in the symptomatic group than in the non-symptomatic group (54.7±12.7 vs. 48.6±10.3, P<0.001; 7.1±3.5 vs. 5.9±2.9, P=0.002; 6.4±3.3 vs. 5.1±3.1, P<0.001; 7.5±4.0 vs. 5.7±3.1, P<0.001). In addition, HADS-depression was correlated with the MFI-K score (P<0.001).Conclusion: There was no evidence of reflux esophagitis causing daytime sleepiness, fatigue, anxiety, or depression, but GERD symptoms were associated with these. Depression was also associated with fatigue. Further studies should clarify the association between fatigue and reflux esophagitis.
Spatial Analysis of Japanese Encephalitis Virus Transmission Risk Factors in Singapore
Authors: Humaidi Mahathir , KWAN Ivan , Xinli YAP , YAP Grace , LIM Xiao Fang , LIAN Jing Er , YEO Gladys , CHONG Chee Seng , Beng HOW Choon , MAILEPESSOV Diyar 
Published: July 2020

Abstract
Background Singapore was previously endemic for JEV but the threat of JE remains. There is continued JEV transmission in animal hosts despite the banning of pig farming in 1992. Surveillance of mosquitoes, ardeid birds and swine gives public health officials useful warning signs for the monitoring of JEV. In this study, we aim to develop a JE Risk Map based on the data gathered by spatial and quantitative survey of the JE-associated vectors and animal hosts. Methods For over a year across the island, the distribution and population of herons were visually surveyed and the distribution of wild boars was captured with wildlife camera traps. Mosquito population data was collected from 16 sites with ecological factors suitable for JE transmission. The animal and vector data were then spatially analysed using open-sourced GIS software. Results Four JE high risk clusters were interpolated from the combined risk factors of data points. A JEV positive Culex tritaeniorynchus pool was subsequently detected in the largest of these JE high risk clusters.
Interferon Regulatory Factor 4 (IRF4) is a Prognostic Marker and Related to Immune Infiltration in Breast and Colorectal Cancers
Authors: Huang Qingyan , Yu Zhikang , Gan Yuhong , Wu Heming , Zhong Zhixiong 
Published: July 2020

Abstract
Background: Interferon regulatory factor 4 (IRF4) is a transcription factor that involves in immune cells differentiation. However, it is not clear the relationship between IRF4 and tumor prognosis and immune infiltration.Methods: IRF4 expression levels in different cancers and corresponding normal tissues were analyzed by Oncomine database and Tumor Immune Estimation Resource (TIMER). The prognosis value of IRF4 was assessed by PrognoScan and Kaplan-Meier plotter. The correlation between IRF4 and tumor-infiltrating immune cells and immune cells markers was performed by TIMER and Gene Expression Profiling Interactive Analysis (GEPIA). In addition, we explored the genes regulated by IRF4 in Gene Transcription Regulation Database (GTRD) and then put the above genes in Enrich online tool for Gene Ontology (GO) and pathway enrichment analysis.Results: Decreased expression levels of IRF4 were observed in breast and colorectal cancers. Survival analysis shown that high level of IRF4 was associated with better prognostic outcome in breast and colorectal cancer patients. IRF4 expression was positively correlated with infiltrating levels of B cells, CD8+ T cells, T cells (general), dendritic cells (DCs), Th1, T cell exhaustion and monocytes, and immune cells markers. Beside, functional enrichment analysis of the potential genes regulated by IRF4 indicated that IRF4 may be involved in many important biological processes including immune regulation by regulating various genes.Conclusions: High expression of IRF4 shown better prognostic outcome for breast and colorectal cancers. IRF4 was associated with immune infiltration in breast and colorectal cancers. Therefore, IRF4 maybe serve as a potential prognostic biomarker in breast and colorectal cancers with immune infiltration.
MiR-18a-5p promotes proliferation, migration and invasion of hepatocellular carcinoma by targeting CPEB3
Authors: Zheng Jianxing , Wu Dongyang , Wang Libing , Qu Fengzhi , Cheng Daming , Wu Xiaotang , Cheng Ling , Wei Yanbing , Liu Xiaogang 
Published: July 2020

Abstract
Objective

The study aims to explore the mechanism of miR-18a-5p targeting CPEB3 gene in regulating the occurrence and development of hepatocellular carcinoma (HCC).
Methods

Differential and survival analyses were conducted on HCC expression profiles from TCGA database to screen out target miRNAs on which targeted prediction was conducted. qRT-PCR was used to detect the expressions of miR-18a-5p and CPEB3. MTT assay examined the proliferation activity, wound healing assay analyzed the migration ability and Transwell assay detected the invasion ability of HCC cells after overexpressing miR-18a-5p.Dual luciferase assay verified the targeting relationship between miR-18a-5p and CPEB3. Meanwhile, MTT, wound healing and Transwellassays determined whether the overexpression of CPEB3 reversed the promoting effect of miR-18a-5p on HCC cells.
Results

Bioinformatic analysis showed that miR-18a-5p was significantly highly expressed in HCC tissues and its target binding site was found in CPEB3 genewith low expression.The qRT-PCR found that high miR-18a-5p expression was observed in HCC cells, and the expression of CPEB3 was significantly low. Overexpression of miR-18a-5p promoted proliferation, migration and invasion of HCC cells. Dual luciferase assay observed that miR-18a-5p inhibited the expression of CPEB3 while overexpression of CPEB3 reversed the promoting effect of miR-18a-5p on the growth of HCCcells.
Conclusion

miR-18a-5p promoted the proliferation and migration of HCC cells by inhibiting the expression of CPEB3. The role of miR-18a-5p /CPEB3 in HCCfound in this study provided a new potential target for the prognostic treatment of HCC patients.