bluuebunny/crossref_metadata_2025_split huggingface dataset:
Exploring patient satisfaction after operative and nonoperative treatment for midshaft clavicle fractures: a focus group analysis
Authors: Tutuhatunewa Eric Danjel , Stevens Martin , Dams Olivier C. , Son Jeffrey van , Louhanepessy Rebecca D. , Krabbe Paul F.M. , Postma Maarten J. , Diercks Ron L. 
Published: March 2020

Abstract
BackgroundThere is no consensus on the optimal treatment for displaced midshaft clavicle fractures. Several studies indicate superior patient satisfaction in favour of operative reconstruction. It is unknown what drives superior satisfaction in this treatment group. The aim of this study was to explore patient satisfaction and identify contributors to patient satisfaction after operative and nonoperative treatment for displaced midshaft clavicle fractures in adults using a focus group approach.MethodsFour face-to-face and two web-based focus groups were hosted. A total of 24 participants who were treated nonoperatively (n = 14) or operatively (n = 10) agreed to participate. Participants were selected using purposive sampling, ensuring variation in gender, age, treatment complications and outcomes. A question script was developed to systematically explore patient expectations, attitudes and satisfaction with different dimensions of care. All focus groups were voice-recorded and transcribed at verbatim. Thematic analysis was conducted on all face-to-face and web-based transcripts. ResultsThe main emerging themes across treatment groups were; need for more information, functional recovery, speed of recovery and patient-doctor interaction. There was no difference in themes observed between operative and nonoperative focus groups. The lack of information was the most important complaint in dissatisfied patients. Conclusion Our study shows that informing patients about their injury, treatment options and expectations for recovery is paramount for overall patient satisfaction after treatment for a displaced midshaft clavicle fracture.
A Family Cluster of Severe Acute Respiratory Syndrome Coronavirus 2 Infections
Authors: Gao Xuesong , Yuan Zheng , Yang Di , Li Hongjie , Zhang Yijin , Gao Ping , Liu Xiaomin , Zhao Wenshan , Xiao Te , Duan Xuefei 
Published: March 2020

Abstract
Background In December 2019, the outbreak of 2019 novel coronavirus disease (COVID-19) began in Wuhan, Hubei Province, China. The disease has rapidly spread from Wuhan to other provinces and other countries. Clinical and epidemiological knowledge is limited. We aimed to describe the clinical and epidemiological characteristics of a family cluster of COVID-19 in Beijing.Methods We reported a family cluster of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) cases in Beijing, China. We obtained and analyzed clinical and epidemiologic data from these patients. Throat swab samples were tested for SARS-CoV-2 using real-time reverse transcriptase-polymerase chain reaction assays.Results This family comprised three laboratory confirmed cases with clinical symptoms. All three patients had close contact with a relative from Wuhan, Hubei Province. Chest computerized tomography revealed ground-glass opacities and consolidation. Patient 1 was finally intubated and mechanical ventilated. Patient 2 received high-flow oxygen therapy. The clinical condition of Patient 3 has resolved.Conclusion SARS-CoV-2 infections tend to clusters. Advanced age and comorbidities are associated with adverse outcomes. Physicians should be aware of contact history so that infected patients can be identified promptly and further spreading prevented.
Effect of livelihood diversification on rural households’ poverty reduction in Central Zone of Tigray regional state, Ethiopia
Authors: Gebretsadik Yohannes Halefom , Teklemariam Berhane Tsegay , Gebru Hailay Nigusie 
Published: March 2020

Abstract
Rural livelihood diversification studies previously conducted in Ethiopia lack linkages with poverty. This study was aimed at investigating the effect of livelihood diversification on rural households’ poverty reduction. The result of this study revealed that 49.80%, 40.41% and 9.80% of the rural households’ income was generated from: agriculture, both agricultural & non-agricultural and non-agricultural income sources respectively. The mean rural livelihood diversification index (SDI) was 0.38. This low diversification index was a source of vulnerability to the adverse effects of climate changes. The absolute poverty line was calculated 6067.75 Birr/year per adult equivalents. Hence, 39.58% of female-headed households, 30.96% male-headed households and 32.65% of the population were poor. The logistic marginal effect analysis indicated that as the households’ SDI increased by one unit, the probability of being poor was reduced by 0.282 (p<0.01%). To reduce rural households' poverty, diversification of rural livelihood beyond agricultural income sources should be promoted through skill training, saving mobilization, improving access to credit, market information, and rural transport facility. Moreover, an appropriate institutional arrangement should be made for promoting a rural non-farm economy that can be possible by capacitating and extending of the small and micro-enterprises development agency into rural areas.
Overexpression of HOXA10 is associated with unfavorable prognosis of acute myeloid leukemia
Authors: Guo Chao , Ju Qian-qian , Zhang Chun-xia , Gong Ming , Li Zhen-ling , Gao Ya-yue 
Published: March 2020

Abstract
Background HOXA family genes were crucial transcription factors involving cell proliferation and apoptosis. While few studies have focused on HOXA10 in AML. We aimed to investigate the prognostic significance of HOXA10. Methods We downloaded datasets from GEO and BeatAML database, to compare HOXA expression level between AML patients and controls. Kaplan-Meier curves were used to estimate the impact of HOXA10 expression on AML survival. The differentially expressed genes, miRNAs, lncRNAs and methylated regions between HOXA10-high and -low groups were obtained using R (version 3.6.0). Accordingly, the gene set enrichment analysis (GSEA) was accomplished using MSigDB database. Moreover, the regulatory TFs/microRNAs/lncRNAs of HOXA10 were identified. A LASSO-Cox model fitted OS to clinical and HOXA10-associated genetic variables by glmnet package. Results HOXA10 was overexpressed in AML patients than that in controls. The HOXA10-high group is significantly associated with shorter OS and DFS. A total of 1219 DEGs, 131 DEmiRs, 282 DElncRs were identified to be associated with HOXA10. GSEA revealed that 12 suppressed and 3 activated pathways in HOXA10-high group. Furthermore, the integrated regulatory network targeting HOXA10 was established. The LASSO-Cox model fitted OS to AML-survival risk scores, which included age, race, molecular risk, expression of IKZF2/LINC00649/LINC00839/FENDRR and has-miR-424-5p. The time dependent ROC indicated a satisfying AUC (1-year AUC 0.839, 3-year AUC 0.871 and 5-year AUC 0.813). Conclusions Our study identified HOXA10 overexpression as an adverse prognostic factor for AML. The LASSO-COX regression analysis revealed novel prediction model of OS with superior diagnostic utility.
Urtica dioica Extract Inhibits Cell Proliferation and Induces Apoptosis in HepG2 and HTC116 as Gastrointestinal Cancer Cell Lines
Authors: Kardan Mostafa , Rafiei Alireza , Golpour Monireh , Ebrahimzadeh Mohammad Ali , Akhavan-Niaki Haleh , Fattahi Sadegh 
Published: March 2020

Background:
Nowadays the use of plant-derived products has been extensively examined in the treatment
of many types of gastrointestinal cancers such as hepatocarcinoma and colon cancer. Urtica dioica is a traditional
herb that has many pharmacological effects and wildly used as a therapeutic agent in cancer. Herein, we have
evaluated the effects of the different concentrations of Methanolic Extract of Urtica dioica (MEUD) on viability,
death pattern, and expression of the apoptosis-related gene in normal Human Dermal Fibroblast (HDF), hepatocarcinoma
cell lines (HepG2) and colon-cancer cell line (HCT116).

Methods:
A high-performance liquid chromatography method was developed to simultaneously separate 3 phenolic
acids in MEUD. HepG2 and HCT116 cell lines as well as HDF normal cell line were cultured in suitable media.
After 24 and 48h, in the cultured cell with different concentrations of MEUD, cells viability was assessed by MTT
assay, and apoptosis was also evaluated at the cellular level by Annexin V/PI flow cytometry analyzing and AO/EB
staining. BCL2 and BAX gene expressions were assessed by TaqMan real-time PCR assay.

Results:
MEUD showed antiproliferative effects on HepG2 and HTC116 cells after 48h with an IC50 value of about
410 and 420μg/ml, respectively (P < 0.001). Apoptotic cells were observed in HepG2 and HTC116 cells but not in
HDF. Furthermore, the increased level of BAX/BCL-2 ratio was observed in HepG2 and HTC116 cells under the
treatment of different concentrations of MEUD.

Conclusions:
The MEUD may influence hepatocarcinoma and colon-cancer cell lines at specific doses and change
their proliferation rate by changing the expression of BAX and BCL2.
Construction and Cloning of Plastic-degrading Recombinant Enzymes (MHETase)
Authors: Janatunaim Rifqi Z. , Fibriani Azzania 
Published: March 2020

Background:Polyethylene terephthalate (PET) is the most widely produced polyester plastic in the world. PET is very difficult to catalyze or biological depolymerization due to the limited access to ester bonds. Consequently, plastic will be stockpiled or flowed into the environment which is projected until hundreds of years. The most effective and environmental friendly plastic degradation method is biodegradation with microorganisms. Two specific enzyme for PET hydrolase, PETase and MHETase have been identified from Ideonella sakaiensis 201-F6. Recombinant genes are made to increase the effectiveness of enzymes in degrading PET. Previous studies of the PETase gene have been carried out, but to produce the final degradation PET product, the enzyme MHETase is needed. Thus, in this study the MHETase gene construction was carried out.Methods:The goal of this study is to construct MHETase gene in pUCIDT plasmid with native signal peptide from I. sakaensis 201-F6 and constitutive promoter J23106 was expressed in Escherichia coli BL21 (DE3) by heats shock. Expression analysis using SDS-PAGE and activity of enzyme is analyzed by spectrophotometry method and SEM.Results:MHETase gene protein was successfully constructed in pUCIDT +Amp plasmid with native signal peptide from Ideonella sakaensis 201-F6, T7 terminator and constitutive promoter J23106. PCR analysis showed that the gene successfully contained in the cells by band size (1813 bp) in electrophoresis gel. Analysis using Snap Gene, pairwise alignment using MEGA X, and NCBI was demonstrated that MHETase sequence the gene was in-frame in pUCIDT plasmid.Conclusion:MHETase gene was successfully constructed in plasmids by in silico method. Synthetic plasmids transformed in E. coli BL21 (DE3) contain MHETase gene sequences which were in frame. Hence, the E. coli BL21 (DE3) cells have the potential to produce MHETase proteins for the plastic degradation testing process. We will patent the construct of MHETase gene using constitutive promoter and signal peptide from native which expressed in E. coli BL21 (DE3). This patent refers to a more applicable plastic degradation system with a whole cell without the need for purification and environmental conditioning of pure enzymes.
Simple storage of sperm cells using combination of coconut and glycerol water towards motility and viability of Koi sperm (Cyprinus carpio)
Authors: Untsa Agista Tsani , Sutarjo Ganjar Adhywirawan , Hakim Riza Rahman 
Published: March 2020

Fish sperm is a crucial part of fish reproduction, especially for Cyprinus carpio. This study aimed to determine the effect of coconut water and glycerol on the motility and viability of sperm koi fish (Cyprinus carpio) and the best treatment on sperm storage of Showa koi fish.This research was conducted for 45 days at Installation ofPunten Freshwater Cultivation - Malang. Thecompletely randomized design (CRD) used with five treatments and three replications. The treatment comes in 750µl coconut water, 500µl coconut water, and 250µl glycerol, 375µl coconut water, and 375µl glycerol, 250µl coconut water and 500µl glycerol, and 750µl glycerol (P1, P2, P3, P4, and P5, respectively). Briefly, the spermis stored at a low temperature (4℃). Observation of sperm motility used sperm cell dilution contained physiologicalNaCl, while the viability calculation used eosin 2% by the smear method. Motility and viability investigationwere employedwere carried out under a microscope with 400x magnification. The results revealed that the addition of coconut water and glycerol had a significant difference on the motility and viability of sperm koi fish. It could survive 96 hours of storage, but the longer motility and viability would decrease. The P3 showed the best result compared to others. The P3 had an average motility scale of 96 - 5.00 and the average viability is 87.38%. Based on it, the treatment could increase the motility and viability of koi fish sperm.
Incidence and Associations of Chronic Kidney Disease in Community Participants With Diabetes: A 5-Year Prospective Analysis of the EXTEND45 Study
Authors: Sukkar Louisa , Kang Amy , Hockham Carinna , Young Tamara , Jun Min , Foote Celine , Pecoits-Filho Roberto , Neuen Brendon , Rogers Kris , Pollock Carol , Cass Alan , Sullivan David , Wong Germaine , Knight John , Peiris David , Gallagher Martin , Jardine Meg 
Published: March 2020

OBJECTIVE
To determine the incidence of and factors associated with an estimated glomerular filtration rate (eGFR) &lt;60 mL/min/1.73 m2 in people with diabetes.

RESEARCH DESIGN AND METHODS
We identified people with diabetes in the EXamining ouTcomEs in chroNic Disease in the 45 and Up Study (EXTEND45), a population-based cohort study (2006–2014) that linked the Sax Institute’s 45 and Up Study cohort to community laboratory and administrative data in New South Wales, Australia. The study outcome was the first eGFR measurement &lt;60 mL/min/1.73 m2 recorded during the follow-up period. Participants with eGFR &lt; 60 mL/min/1.73 m2 at baseline were excluded. We used Poisson regression to estimate the incidence of eGFR &lt;60 mL/min/1.73 m2 and multivariable Cox regression to examine factors associated with the study outcome.

RESULTS
Of 9,313 participants with diabetes, 2,106 (22.6%) developed incident eGFR &lt;60 mL/min/1.73 m2 over a median follow-up time of 5.7 years (interquartile range, 3.0–5.9 years). The eGFR &lt;60 mL/min/1.73 m2 incidence rate per 100 person-years was 6.0 (95% CI 5.7–6.3) overall, 1.5 (1.3–1.9) in participants aged 45–54 years, 3.7 (3.4–4.0) for 55–64 year olds, 7.6 (7.1–8.1) for 65–74 year olds, 15.0 (13.0–16.0) for 75–84 year olds, and 26.0 (22.0–32.0) for those aged 85 years and over. In a fully adjusted multivariable model incidence was independently associated with age (hazard ratio 1.23 per 5-year increase; 95% CI 1.19–1.26), geography (outer regional and remote versus major city: 1.36; 1.17–1.58), obesity (obese class III versus normal: 1.44; 1.16–1.80), and the presence of hypertension (1.52; 1.33–1.73), coronary heart disease (1.13; 1.02–1.24), cancer (1.30; 1.14–1.50), and depression/anxiety (1.14; 1.01–1.27).

CONCLUSIONS
In participants with diabetes, the incidence of an eGFR &lt;60 mL/min/1.73 m2 was high. Older age, remoteness of residence, and the presence of various comorbid conditions were associated with higher incidence.
Sistema de robótica industrial en operaciones agroindustriales: optimización del proceso de limpieza y selección de frutas
Authors: García Sánchez Roberto , García Vacacela Roberto Carlos , Villavicencio Bermudes Nicolás , Meléndez Rangel Jesús 
Published: March 2020

Este artículo derivado de una investigación cuantitativa, de tipo cuasi experimental, planteó estudiar el uso del tiempo con la simulación del sistema de robótica industrial en la limpieza y selección de frutas. La población estuvo conformada por la cantidad diaria de unidades de mango (900) y piñas (700) procesadas en un período de 26 semanas de trabajo. La información provino de las estadísticas gestionadas y mantenidas por Agrocalidad, los datos unificados y consolidados por el Sistema de Información Nacional del Ministerio de Agricultura, Ganadería, Acuacultura y Pesca (SINAGAP) y la información que aportó la simulación de robótica industrial, implementada con software de ABB Robotics. Una vez obtenido el conjunto informativo se utilizó el paquete estadístico R 3.2.x. El grupo G1 estuvo conformado por 55 días de trabajo de una empacadora promedio. Mientras que el grupo G2 fue designado por los registros de tiempos empleados en las dos (2) partes del proceso de limpieza en los 55 días. Se concluye que el proceso tradicional manual de limpieza y selección de frutas, incrementa la cantidad de tiempo usado por unidad; mientras que en el proceso automatizado con robótica industrial, mantiene una tendencia a la estabilidad entre los 3,2 minutos para el mango y 2,7 minutos para la piña.
РЕПЕРТУАР ШОРСКОГО КАЙЧИ В. Е. ТАННАГАШЕВА
Authors: Arbachakova Ljubov 
Published: March 2020

Сегодня, когда живая сказительская традиция алтае-саянских народов почти угасла, нам удалось сделать аудиозаписи героических сказаний в исполнении представителя мрасской сказительской школы – В. Е. Таннагашева (1932–2007 гг.). По учтённым записям в репертуаре сказителя насчитывается 72 героических сказания, из них 37 – сделаны в аудио и видеозаписи, а 35 – в самозаписи кайчи. 16 эпических произведений из этого репертуара не имеют вариантов. Имеющиеся аудио и видеозаписи сказаний В. Е. Таннагашева были сделаны в разные годы Л. Н. Арбачаковой и Д. А. Функом. Имеются отдельные фиксации сказаний В. Е. Таннагашева, произведённые другими собирателями. Всё это составляет прекрасную источниковую базу для исследования творчества одного из выдающихся современных сказителей Горной Шории.Today, when living story-tellers tradition of the Altai-Sayan peoples gradually fade away, we were able to make audio recordings of heroic legends in a performance of representative of Mrass story-tellers school V. Tannagashev (1932–2007). According to ledger recordings, in the repertoire of the storyteller 72 heroic legends, among them 37 made audio and video recordings, and 35 self writing of story-teller (kajchi), 16 epic stories do not have options. Available audio and video recordings of legends of V. Tannagashev, made by L. N. Arbachakova and D. A. Funk. There are some fixing stories, produced other collectors provide a good background for deep research, dedicated to the creativity of one of the greatest storytellers of Mountain Shoria.
DOĞU AKDENİZ’DE DENİZ YETKİ ALANLARI VE ENERJİ KAYNAKLARI ÇERÇEVESİNDE TÜRKİYE’NİN ENERJİ GÜVENLİĞİ
Authors: PEKER Hasan Sencer , ÖZTÜRK OKTAY Kübra , ŞENSOY Yavuz 
Published: March 2020

Dünyada artan
enerji ihtiyacı ve bağımlılığı; tarihsel önemini yüzyıllardır koruyan Doğu
Akdeniz’i günümüzde tekrar diplomasinin merkezine çekmeye başlamıştır. Doğu
Akdeniz’de bulunan hidrokarbon rezervleri kıyıdaş devletlerin bu rezervler
üzerinde arama ve yararlanma hakkı iddia etmeleri ve bu iddia edilmiş haklarını
hukuki zemine oturtabilmek için ikili anlaşmalar yapmalarına sebep olmuştur.
Ancak bu rezervlerden yararlanma hakkının hem Kuzey Kıbrıs Türk Cumhuriyeti’nin
varlığını hem de Türkiye ve Kuzey Kıbrıs Türk Cumhuriyeti arasındaki ikili
anlaşmayı göz ardı etmesi sebebiyle bölgede bir takım gerilimler ortaya
çıkmaktadır. Kıbrıs adasının hukuki durumu da göz önünde bulundurulduğunda,
rezervlerden elde edilen ekonomik değerlerin hem ada halkının ortak çıkarına
sunulmaması hem de Türkiye’nin Kuzey Kıbrıs Türk Cumhuriyeti ile anlaşmasından
doğan yetki alanlarının gasp edilmesi açısından önemi bulunmaktadır.

Bu çalışmada,
Doğu Akdeniz’deki tarafların iddia ettiği yetki alanları ve bu alanlardan elde
edilen çıktının paylaşımı uluslararası hukuk nezdinde bir zemine oturtulmaya
çalışılacaktır. Bu sebeple önce Doğu Akdeniz’deki yetki alanlarının
Uluslararası Deniz Hukuku’na uygun biçimde ayrımı yapılacak olup sonrasında
Kıbrıs adasının ve Türkiye’nin Doğu Akdeniz’deki konumuna değinilmiştir. Güney
Kıbrıs Rum Yönetimi’nin adanın tek söz sahibi ve temsilcisi gibi davranmasının
uluslararası hukuka aykırılığından bahsedilecek olup olası çözüm yollarına da
değinilmiştir.
Multi-objective Optimisation of Gene Regulatory Networks: Insights from a Boolean Circadian Clock Model
Authors: Akman Ozgur Ekim , Fieldsend Jonathan Edward 
Published: March 2020

The gene regulatory networks that comprise circadian clocks modulate biological function across a range of scales, from gene expression to performance and adaptive behaviour. These timekeepers function by generating endogenous rhythms that can be entrained to the external 24-hour day-night cycle, enabling organisms to optimally time biochemical processes relative to dawn and dusk. In recent years, computational models based on differential equations, and more recently on Boolean logic, have become useful tools for dissecting and quantifying the complex regulatory relationships underlying the clock’s oscillatory dynamics. Optimising the parameters of these models to experimental data is, however, non-trivial. The search space is continuous and increases exponentially with system size, prohibiting exhaustive search procedures, which are often emulated instead via grid-searching or random explorations of parameter space. Furthermore, to simplify the search procedure, objective functions representing fits to individual experimental datasets are often aggregated, meaning the information contained within them is not fully utilised.Here, we examine casting this problem as a multi-objective one, and illustrate how the use of an evolutionary optimisation algorithm — the multi-objective evolution strategy (MOES) — can significantly accelerate the parameter search procedure. As a test case, we consider an exemplar circadian clock model based on Boolean delay equations — dynamic models that are discrete in state but continuous in time. The discrete nature of the model enables us to directly compare the performance of our optimiser to grid searches based on enumeration of the parameter space at a fixed resolution. We find that the MOES generates near-optimal parameterisations in computation times which are several orders of magnitude faster than the grid search. As part of this investigation, we also show that there is a distinct trade-off between the performance of the clock circuit in free-running and entrained photic environments. Importantly, runtime results indicate that the use of multi-objective evolutionary optimisation algorithms will make the investigation of larger and more complex models computationally tractable.