bluuebunny/crossref_metadata_2025_split huggingface dataset:
Two Cobalt Chelatase Subunits Can Be Generated from a Single chlD Gene via Programed Frameshifting
Authors: Antonov Ivan V 
Published: March 2020

Abstract
Magnesium chelatase chlIDH and cobalt chelatase cobNST enzymes are required for biosynthesis of (bacterio)chlorophyll and cobalamin (vitamin B12), respectively. Each enzyme consists of large, medium, and small subunits. Structural and primary sequence similarities indicate common evolutionary origin of the corresponding subunits. It has been reported earlier that some of vitamin B12 synthesizing organisms utilized unusual cobalt chelatase enzyme consisting of a large cobalt chelatase subunit (cobN) along with a medium (chlD) and a small (chlI) subunits of magnesium chelatase. In attempt to understand the nature of this phenomenon, we analyzed &gt;1,200 diverse genomes of cobalamin and/or chlorophyll producing prokaryotes. We found that, surprisingly, genomes of many cobalamin producers contained cobN and chlD genes only; a small subunit gene was absent. Further on, we have discovered a diverse group of chlD genes with functional programed ribosomal frameshifting signals. Given a high similarity between the small subunit and the N-terminal part of the medium subunit, we proposed that programed translational frameshifting may allow chlD mRNA to produce both subunits. Indeed, in genomes where genes for small subunits were absent, we observed statistically significant enrichment of programed frameshifting signals in chlD genes. Interestingly, the details of the frameshifting mechanisms producing small and medium subunits from a single chlD gene could be prokaryotic taxa specific. All over, this programed frameshifting phenomenon was observed to be highly conserved and present in both bacteria and archaea.
Killing Time Again
Authors: Vihvelin Kadri 
Published: March 2020

Abstract
I have argued that even if time travel is metaphysically possible, there are some things a time traveler would not be able to do. I reply here to critics who have argued that my account entails fatalism about the past or entails that the time traveler is unfree or that she is bound by “strange shackles.” My argument does not entail any sort of fatalism. The time traveler is able to do many of the things that everyone else can do and is as free as any non-time-traveler. The time traveler is constrained only as we all are by the laws of nature. My argument shows only how strangely those constraints must operate if those laws permit time travel.
Health-related quality of life and symptoms of conservative care versus dialysis in patients with end-stage kidney disease: a systematic review
Authors: Verberne Wouter R , van den Wittenboer Iris D , Voorend Carlijn G N , Abrahams Alferso C , van Buren Marjolijn , Dekker Friedo W , van Jaarsveld Brigit C , van Loon Ismay N , Mooijaart Simon P , Ocak Gurbey , van Delden Johannes J M , Bos Willem Jan W 
Published: March 2020

AbstractBackgroundNon-dialytic conservative care (CC) has been proposed as a viable alternative to maintenance dialysis for selected older patients to treat end-stage kidney disease (ESKD). This systematic review compares both treatment pathways on health-related quality of life (HRQoL) and symptoms, which are major outcomes for patients and clinicians when deciding on preferred treatment.MethodsWe searched PubMed, Embase, Cochrane Library, Cumulative Index to Nursing and Allied Health Literature (CINAHL) Plus and PsycINFO from inception to 1 October 2019 for studies comparing patient-reported HRQoL outcomes or symptoms between patients who chose either CC or dialysis for ESKD.ResultsEleven observational cohort studies were identified comprising 1718 patients overall. There were no randomized controlled trials. Studies were susceptible to selection bias and confounding. In most studies, patients who chose CC were older and had more comorbidities and worse functional status than patients who chose dialysis. Results were broadly consistent across studies, despite considerable clinical and methodological heterogeneity. Patient-reported physical health outcomes and symptoms appeared to be worse in patients who chose CC compared with patients who chose dialysis but had not yet started, but similar compared with patients on dialysis. Mental health outcomes were similar between patients who chose CC or dialysis, including before and after dialysis start. In patients who chose dialysis, the burden of kidney disease and impact on daily life increased after dialysis start.ConclusionsThe available data, while heterogeneous, suggest that in selected older patients, CC has the potential to achieve similar HRQoL and symptoms compared with a dialysis pathway. High-quality prospective studies are needed to confirm these provisional findings.
Macrophage interactions with collecting duct epithelial cells are capable of driving tubulointerstitial inflammation and fibrosis in immunoglobulin A nephropathy
Authors: Pawluczyk Izabella Z A , Soares Maria S F , Barratt William A , Brown Jeremy R , Bhachu Jasraj S , Selvaskandan Haresh , Zeng Yiqing , Sarania Rishi , Molyneux Karen , Roberts Ian S D , Barratt Jonathan 
Published: March 2020

AbstractBackgroundTubulointerstitial fibrosis is a powerful predictor of future progression inimmunoglobulin A (IgA) nephropathy (IgAN). Proximal tubular epithelial cells (PTECs), in concert with infiltrating macrophages, are regarded as the agents provocateurs for driving this fibrotic process. However, evidence is now emerging for a contributory role of the distal nephron. The aim of this study was to examine the potential influence of macrophages on collecting duct epithelial cells (CDECs) and their combined role in the progression of IgAN.MethodsCDECs were cultured with macrophage-conditioned media (MCM) generated from human monocyte cell lines U937 and THP-1 stimulated with or without 100 μg/mL galactose-deficient IgA1. CDECs were analysed for evidence of inflammation and fibrosis.ResultsStaining of IgAN biopsies for CD68+ macrophages revealed the presence of macrophages juxtaposed to collecting ducts and within their lumina. CDEC exposed to MCM from IgA1-stimulated THP-1 cells (THP-1-IgA-MCM) exhibited markedly increased expression of neutrophil-associated gelatinase (NGAL) and proinflammatory cytokinesinterleukin (IL)-1β, tumour necrosis factor-α, IL-6 and IL-8 compared with MCM from non-IgA-stimulated THP-1 cells (THP-1-MCM). U937-IgA-MCM increased fibronectin levels and reduced E-cadherinmRNA expression. THP-1-IgA-MCM-derived exosomes induced similar increases in NGAL and cytokine expression while in cross-over experiments exosomes extracted from IL-1β-exposed CDEC induced IL-1β and IL-6 mRNA expression in both sets of macrophages. MiRnome analysis revealed that microRNA (miR)-146a, -155 and -200b exhibited a &gt;2-fold increase in expression in CDEC treated with THP-1-IgA-MCM compared with THP-1-MCM. Enforced miR-146a suppression further enhanced NGAL expression, while ectopic miR-146a over-expression downregulated it. NGAL mRNA and miR-146a were upregulated in the biopsies of patients with progressive IgAN compared with non-progressive IgAN.ConclusionsTaken together, these data suggest that CDEC–macrophage interactions potentially contribute to the tubulointerstitial fibrosis characteristic of progressive IgAN.
Trends and Associations of Menthol Cigarette Smoking Among US Middle and High School Students—National Youth Tobacco Survey, 2011–2018
Authors: Sawdey Michael D , Chang Joanne T , Cullen Karen A , Rass Olga , Jackson Kia J , Ali Fatma Romeh M , Odani Satomi , Courtney-Long Elizabeth A , Armour Brian S , Ambrose Bridget K , Agaku Israel T 
Published: March 2020

Abstract

Introduction
Youth cigarette smoking decreased significantly over the last two decades in the United States. This study provides estimates and trends from 2011 to 2018 and factors associated with youth menthol and non-menthol smoking from 2016 to 2018.

Methods
Using data from the 2011–2018 National Youth Tobacco Surveys, past 30-day (current) menthol and non-menthol cigarette smoking were estimated for all youth (prevalence) and youth smokers (proportions). Trends were examined using Joinpoint regression, calculating the annual percent change (APC). Multivariate logistic regression analyses identified factors associated with menthol smoking.

Results
From 2011 to 2018, menthol cigarette smoking among current youth cigarette smokers significantly decreased from 57.3% to 45.7% (APC: −3.0%), while non-menthol (38.2% to 47.3% [APC: 2.9%]) and unknown menthol status (not sure\missing) (4.5% to 7.0% [APC: 7.1%]) significantly increased. Menthol cigarette smoking among high school, male, female, and non-Hispanic white current cigarette smokers decreased, but remained unchanged among middle school, non-Hispanic black, and Hispanic smokers. Significantly higher proportions of menthol cigarette smokers smoked on ≥20 days, ≥2 cigarettes per day, and ≥100 cigarettes in their lifetime compared to non-menthol smokers. Among current cigarette smokers, non-Hispanic blacks, Hispanics, flavored non-cigarette tobacco users, frequent smokers (≥20 days), those smoking 2–5 cigarettes per day, and those living with someone who uses tobacco had higher odds of menthol cigarette smoking.

Conclusions
In 2018, nearly half of current youth cigarette smokers smoked menthol cigarettes. While menthol cigarette smoking declined from 2011 to 2018 among all youth and among youth smokers, there was no change in menthol cigarette smoking among non-Hispanic black, Hispanic, and middle school cigarette smokers.

Implications
This study finds that overall cigarette and menthol cigarette smoking declined in youth from 2011 to 2018. However, menthol cigarette smoking among non-Hispanic black, Hispanic, and middle school youth cigarette smokers did not change. Information from this study can help inform efforts to reduce menthol cigarette smoking among US youth, particularly racial/ethnic minority populations.
Renovating Christian Charity: Global Catholicism, the Save the Children Fund, and Humanitarianism During The First World War*
Authors: Houlihan Patrick J 
Published: March 2020

Abstract
This article argues that the Vatican’s involvement in the fledgling stages of the Save the Children Fund helped globalize what began as a local British charity, reshaping Christian humanitarianism as a response to total war. Centred on children as irreproachable war victims and the hope of the future, the ideology of Christian charity and the Vatican’s financial networks helped mobilize resources to combat famine across shattered imperial state structures in Central and Eastern Europe. With diplomatic credentials as a peace advocate, Pope Benedict XV (1914–22) symbolically led this new wave of religious humanitarianism. Attempting to stabilize war-torn societies, Christian humanitarianism towards children was an ideology that overcame wartime British anti-Germanism, raising fears about the spectre of Bolshevism after the Russian Revolution of 1917. In contrast to 19th-century religious mobilization that hardened confessional boundaries against the liberal secular state, however, this was a moment when the Catholic Church as a global religious organization intervened for all people, irrespective of faith commitments. Influencing later human rights developments, religiously informed humanitarianism became forgotten in the Vatican’s aggressive anti-communist diplomacy in the inter-war era. Ecumenical religious charity was important for the modern history of humanitarianism and non-governmental organizations.
Comparative Effectiveness of Targeted Intrathecal Drug Delivery Using a Combination of Bupivacaine with Either Low-Dose Fentanyl or Hydromorphone in Chronic Back Pain Patients with Lumbar Postlaminectomy Syndrome
Authors: Ade Timothy , Roh Justin , Sharma Gautam , Mohan Mahesh , DeLozier Sarah J , Janes Jessica L , Hayek Salim M 
Published: March 2020

AbstractObjectiveTargeted intrathecal drug delivery (TIDD) is an effective interventional pain management modality often used in postlaminectomy patients with refractory chronic low back pain. A combination of intrathecal bupivacaine with an opioid is often used. However, intrathecal catheter tip granulomas have occurred with use of morphine or hydromorphone but generally not with fentanyl. The objective of this study was to compare the efficacy of TIDD using bupivacaine/fentanyl vs bupivacaine/hydromorphone in patients with chronic intractable low back pain postlaminectomy.Materials and MethodsA retrospective comparative analysis of consecutive patients with lumbar postlaminectomy syndrome who were trialed and later received TIDD with a combination of bupivacaine/hydromorphone or bupivacaine/fentanyl between June 2009 and May 2016 at a single tertiary medical center.ResultsWe identified a cohort of 58 lumbar postlaminectomy patients receiving a TIDD admixture of either hydromorphone/bupivacaine (30 patients) or low-dose fentanyl/bupivacaine (28 patients) with at least two years of follow-up. The fentanyl group had significantly lower baseline opioid consumption and a lower rate of intrathecal opioid dose escalation. Both groups had similar and significant reductions in pain scores over the two-year follow-up period. No granulomas were observed.ConclusionTIDD using a low-dose fentanyl admixture with bupivacaine in patients with postlaminectomy syndrome and refractory chronic low back pain results in similar pain relief to TIDD with hydromorphone and bupivacaine. Low-dose intrathecal fentanyl leads to a lower rate of opioid escalation and may be safer than hydromorphone.
Hamlet and Character in Modernist Criticism
Authors: Murray Nathan 
Published: March 2020

Abstract
This article is a critical history of the conflict over the ‘living character’, especially in the context of the debates over Hamlet between A. C. Bradley, T. S. Eliot and G. Wilson Knight. I argue that Bradley is a nuanced critic, who, far from seeing Hamlet as merely the imitation of a real person, recognizes the role of the reader in filling narrative gaps, smoothing over inconsistencies, and ultimately accepting the incompleteness of the text. I argue that Eliot and Wilson Knight, who attack Hamlet’s character in their writings, should be understood as engaged in the deliberate construction of a teleological metanarrative that presents the destabilized modernist character as the natural culmination of literary history.
Whole exome sequencing in unclassified autoinflammatory diseases: more monogenic diseases in the pipeline?
Authors: Kosukcu Can , Taskiran Ekim Z , Batu Ezgi Deniz , Sag Erdal , Bilginer Yelda , Alikasifoglu Mehmet , Ozen Seza 
Published: March 2020

Abstract

Objective
Autoinflammatory diseases (AIDs) are characterized by recurrent sterile systemic inflammation attacks. More than half of the patients remain genetically undiagnosed with next-generation sequencing panels for common AIDs. In this study, we aimed to define phenotype-genotype correlations in a cohort of unclassified AID patients via whole exome sequencing (WES).

Methods
Patients with features of AIDs were included in this study followed in the Department of Pediatric Rheumatology at Hacettepe University. They were first screened for MEFV with Sanger sequencing and then WES performed for the patients with clinically insignificant results. Pre-analysis of WES data was done by considering the 13 most common AID-related genes. Further bioinformatic analysis was performed if the patient remained genetically undiagnosed.

Results
The median age at disease onset was 1.2 years (range 0.2–16) and at the time of study recruitment was 14 years (range 3.5–17). In our cohort, WES provided a definite or probable disease-causing variant in 4 of 11 patients (36%). Heterozygous mutations for two of these genes were previously associated with neurological defects (ADAM17, TBK1), also homozygous ADAM17 mutations were observed in one family with neonatal inflammatory skin and bowel disease. Besides, two genes (LIG4, RAG1) were associated with immunodeficiency although the patients had presented with inflammatory features. Finally, for one patient, we associated a strong candidate gene (NLRC3) with autoinflammatory features.

Conclusion
WES strategy is cost-effective and provides substantial results for a selected group of undefined AID patients. Our results will contribute to the spectrum of unclassified AIDs.
Origin and evolution of dengue virus type 2 causing outbreaks in Kenya: Evidence of circulation of two cosmopolitan genotype lineages
Authors: Langat Solomon K , Eyase Fredrick Lunyagi , Berry Irina Maljkovic , Nyunja Albert , Bulimo Wallace , Owaka Samuel , Ofula Victor , Limbaso Samson , Lutomiah Joel , Jarman Richard , Distelhorst John , Sang Rosemary C 
Published: March 2020

Abstract
Dengue fever (DF) is an arboviral disease caused by dengue virus serotypes 1-4 (DENV 1-4). Globally, DF incidence and disease burden have increased in the recent past. Initially implicated in a 1982 outbreak, DENV-2 recently reemerged in Kenya causing outbreaks between 2011 and 2014 and more recently 2017–8. The origin and the evolutionary patterns that may explain the epidemiological expansion and increasing impact of DENV-2 in Kenya remain poorly understood. Using whole-genome sequencing, samples collected during the 2011–4 and 2017–8 dengue outbreaks were analyzed. Additional DENV-2 genomes were downloaded and pooled together with the fourteen genomes generated in this study. Bioinformatic methods were used to analyze phylogenetic relationships and evolutionary patterns of DENV-2 causing outbreaks in Kenya. The findings from this study have shown the first evidence of circulation of two different Cosmopolitan genotype lineages of DENV-2; Cosmopolitan-I (C-I) and Cosmopolitan-II (C-II), in Kenya. Our results put the origin location of C-I lineage in India in 2011, and C-II lineage in Burkina Faso between 1979 and 2013. C-I lineage was the most isolated during recent outbreaks, thus showing the contribution of this newly emerged strain to the increased DENV epidemics in the region. Our findings, backed by evidence of recent local epidemics that have been associated with C-I in Kenya and C-II in Burkina Faso, add to the growing evidence of expanding circulation and the impact of multiple strains of DENV in the region as well as globally. Thus, continued surveillance efforts on DENV activity and its evolutionary trends in the region, would contribute toward effective control and the current vaccine development efforts.
High-throughput DNA sequencing of museum specimens sheds light on the long-missing species of theBokermannohyla claresignatagroup (Anura: Hylidae: Cophomantini)
Authors: Lyra Mariana L , Lourenço Ana Carolina C , Pinheiro Paulo D P , Pezzuti Tiago L , Baêta Délio , Barlow Axel , Hofreiter Michael , Pombal José P , Haddad Célio F B , Faivovich Julián 
Published: March 2020

AbstractThe two species of the Bokermannohyla claresignata species group (Anura: Hylidae) have not been collected for the last four decades. It is the only species group of the hyline tribe Cophomantini that has not yet been analysed genetically. Its phylogenetic position is thus uncertain, and it has a combination of adult and larval character states that make this group a crucial missing piece that hinders our understanding of Cophomantini phylogenetics and character evolution. We obtained DNA sequences from a museum larval specimen of Bok. claresignata, using specialized extraction methods and high-throughput DNA sequencing, and combined the molecular phylogenetic results with available phenotypic information to provide new insights into the taxonomy and phylogenetic relationships of its species group. Our phylogenetic results place Bok. claresignata as sister to the Boana pulchella group, supporting its inclusion in Boana, together with Bokermannohyla clepsydra. In light of this new finding, we recognize a newly defined Boana claresignata group to accommodate these species, thus resolving both the polyphyly of Bokermannohyla and the paraphyly of Boana. Considering the phylogenetic relationships of the Boana claresignata group, we also discuss the evolution of suctorial tadpoles and mature oocyte/egg pigmentation in Cophomantini.
Hand Dermatitis in Nursing Students
Authors: Özyazicioğlu Nurcan , Sürenler Semra , Aydin Ayla İrem , Atak Meryem 
Published: March 2020

ABSTRACT

OBJECTIVE
To determine the frequency of self-reported hand dermatitis and the factors influencing its prevalence among nursing students.

METHODS
Researchers collected demographic data and used a self-assessment form to identify dermatologic symptoms. The questionnaires were distributed to the students and collected again after the students completed the forms.

RESULTS
Hand dermatitis was present in 20.9% of nursing students. The most common symptoms were irritation/pruritus, redness/cracking, flaking/rash, swelling, and vesicles in hands. The grade of the student, the presence of allergy complaints, and medication used to treat dermatitis were statistically significantly associated with dermatitis prevalence. The use of gloves and cleansing agents used in handwashing were factors linked to hand dermatitis.

CONCLUSIONS
Hand dermatitis increases in parallel with the increase of clinical practice hours among nursing students. Familial and environmental factors also increase the risk of hand dermatitis.
Using VoiceThread to Build a Community of Inquiry in Blended RN-to-BSN Education
Authors: Merriam Deborah , Hobba-Glose Janice 
Published: March 2020

Abstract
VoiceThread, a cloud-based social media tool, was used to create a sense of community in an undergraduate blended nursing leadership course. The purpose of this quasi-experimental study was to explore the use of VoiceThread to build a community of inquiry. The Community of Inquiry and IDEA surveys were completed by 163 students enrolled in an undergraduate nursing leadership course within an RN-BS curriculum. Results indicated using VoiceThread increased student-perceived teacher excellence, supported social presence and teacher presence, and promoted a community of inquiry.