bluuebunny/crossref_metadata_2025_split huggingface dataset:
Correlates of Sun Protection Behaviors Among Melanoma Survivors
Authors: Heckman Carolyn , Manne Sharon , Kashy Deborah , Ritterband Lee , Bhurosy Trishnee , Coups Elliot 
Published: December 2020

Abstract
Background: The study objective was to assess potential correlates of sun protection behaviors among melanoma survivors.Methods: Participants were 441 melanoma survivors recruited from three health centers and a state cancer registry in the United States. Sun protection behaviors (sunscreen, shade, protective shirts, and hats) were assessed through an online survey, as were potential correlates (demographic, melanoma risk, knowledge and attitudinal, psychological, and social influence factors). Hierarchical multiple regression analyses were conducted. Results: Correlates of sun protection behaviors included education, skin cancer risk factors, melanoma knowledge and attitudes, melanoma worry and distress, physician recommendation for sun protection, injunctive norms, and pro-protection attitudes (e.g., perceived barriers, self-efficacy). Conclusions: Future efforts to improve sun safety among melanoma survivors may benefit from targeting individuals with lower education levels, and addressing sun protection social influence, barriers, and self-efficacy.
Patient Satisfaction and Associated Factors Among Adult Outpatient Ophthalmic Service Users’ at University of Gondar Comprehensive Specialized Hospital Tertiary Eye Care and Training Center, Northwest Ethiopia, 2020: An Institution-Based Cross-Sectional Study
Authors: Lorato Merkineh , Hussen Mohammed , Tegegne Mebratu 
Published: December 2020

Abstract
Background: Patient satisfaction is explained as “the extent to which patients are happy with their health care, both inside and outside of the doctor’s office”. Observed complain and limited evidence on patient satisfaction specific to ophthalmic services. So, aimed to assess the overall patient satisfaction and associated factors among adult outpatient ophthalmic service users at University of Gondar Comprehensive Specialized Hospital, Tertiary Eye Care and Training Center, Northwest Ethiopia, 2020.Methods and materials: An institution-based cross-sectional study was conducted from June 26 to August 5 with a sample size of 414. A systematic random sampling technique was used to select participants. An interviewer administered questionnaire was employed to collect the data. The data was analyzed by SPSS 20. Descriptive statistics such as frequency cross tabulation test was carried out to translate data into information. P-value less than 0.05 was considered as statistically significant. Result: A total of 403 participants with 97.34% response rate were included in this study. Their mean age was 46.67±16.21 years. The majorities were males (59.8%). The overall patient satisfaction was 57.1% at 95% CI :( 52.1%-61.5%). The top three sub-domains with greater score of satisfaction were general outlook (67.5%), communication with providers (64.5%), and interpersonal manner (61.5%). The least satisfaction score was to waiting time (45.9%) sub domain. Being unmarried (AOR = 2.51, 95% CI 1.21-5.23) and earning a monthly income of Ethiopian Birr ≥2500 (AOR = 5.90; 95% CI: 2.11-16.5) were associated with the patient satisfaction.Conclusion: The result infers that nearly sixty percent of the study participants were satisfied. While improving each sub-domain of patient satisfaction, waiting time and financial aspect sub-domains need greater emphasis by the hospital manager to improve patient satisfaction. The hospital manager, policymakers and other stakeholders need to set a quality improvement plan to solve the issue.
The Control Algorithm of Whole Angle Mode for HRG Based on the Vector Composition
Authors: Zhao Wanliang , Cheng Yuxiang , Li Shaoliang , Song Lijun 
Published: December 2020

Abstract
The Hemispherical Resonator Gyroscope (HRG) has many advantages such as high precision, high reliability and long life-time, it is widely used in the space-launcher and the satellites. The HRG has been mechanized to operate in two the distinct operating modes, the Force to Rebalanced (FTR) mode and the Whole Angle (WA) mode. Different from the traditional control algorithm is based on average methods which usually used for WA mode, in the paper, a new electrostatic control algorithm is presented, which based on the vector composition and decomposition method to control the equivalent drive force in order to track the phase of the standing wave. The mathematics model and the control algorithm are presented in the paper. And the hardware experimental circuit system is implemented, the HRG has a range of more than 300°/s with the linearity of 40 ppm. Meanwhile, the energy transition efficiency increases by an order of magnitude, rather than the average methods.
“We are not Hard to Reach, but we may find it Hard to Trust”…. Involving and Engaging ‘Seldom Listened to’ Community Voices in Clinical Translational Health Research: A Social Innovation Approach.
Authors: Islam Safina , Joseph Olivia , Chaudry Atiha , Forde Davine , Keane Annie , Wilson Cassia , Begum Nasima , Parsons Suzanne , Grey Tracy , Holmes Leah , Starling Bella 
Published: December 2020

Abstract
Background: Public involvement in clinical translational research is increasingly recognized as essential for relevant and robust research. Public involvement must be diverse and inclusive to enable research that has the potential to reach those that stand to benefit from it the most, and thus address issues of health equity. Several recent reports, however, indicate that public involvement is exclusive, including in its interactions with ethnic groups. This paper outlines a novel community-led methodology – a community sandpit – to address the inclusion of ethnic groups in public involvement in research, reports on its evaluation, findings, legacy and impact.Methods: Through careful planning, relationship-building, co-design and co-delivery between the Public Programmes team based at Manchester University NHS Foundation Trust and the Greater Manchester Black and Minority Ethnic Network, the community sandpit was held in July 2018. Results: 15 community organisations took part in the participatory 2-day event, as well as 6 researchers, and 6 creative practitioners. 6 community-based partnership projects were seed-funded; 4 of these carried on to receive additional funding.Conclusions: Evaluation of the sandpit showed the format to be well-received by all: it levelled power relationships between community organisations, health researchers and research infrastructure; it developed capacity amongst researchers about the accessibility, role and potential of community organisations. Described as “not another community seed fund” by community partners, the sandpit offered equitable avenues for collaboration within Greater Manchester translational research and led to the formation of the Black, Asian and Minority Ethnic Research Advisory Group. The method has the potential to be replicated elsewhere to support inclusive public involvement in research and inclusive research.
A Case of Primary Hepatic Extranodal Marginal Zone B-Cell Lymphoma of Mucosa-Associated Lymphoid Tissue (MALT) Type Treated by Radiofrequency Ablation (RFA) and Literature Review
Authors: Xu Zhe , Pang Chong , Sui Jidong , Gao Zhenming 
Published: December 2020

Abstract
BackgroundPrimary hepatic mucosa-associated lymphoid tissue (MALT) lymphoma is an extremely rare liver malignancy and is usually lack characteristic imaging findings, which is often misdiagnosed. Case presentationThe present study reports a case of a 63-year-old woman diagnosed with primary hepatic extranodal marginal zone B-cell lymphoma of MALT type. The patient received the needle biopsy and radiofrequency ablation and has not shown relapse up for the following 12 months.ConclusionsThis case is reported for its rarity and the treatment for this patient is unique and effective. Our case provides a new option for the treatment of hepatic MALT lymphoma. We also present our findings from a systematic review to improve our current understanding of the disease.
Improving the Anthocyanin Accumulation of Hypocotyls in Radish Sprouts by Hemin-induced NO
Authors: Su Nana , Liu Ze , Chen Hui , Niu Mengyang , Cui Jin 
Published: December 2020

Abstract
Background: The biosynthesis of anthocyanin in the hypocotyls of radish (Raphanus sativus L.) sprouts was enhanced by hemin in our preliminary experiments, but the underlying mechanism is unclear. Here, we found that NO (nitric oxide) exerted an essential role in Hemin-regulated anthocyanin biosynthesis, which was supported by the following results.Results: Hemin boosted anthocyanin as well as NO content. NO-scavenger cPTIO (carboxy-PTIO) significantly attenuated hemin-induced increase of anthocyanin content, transcripts of anthocyanin synthesis related genes and positive transcription factors, implying that NO played a prominent role during hemin-induced anthocyanin biosynthesis. Hemin specific inhibitor ZnPP (Zinc Protoporphyrin) strongly reduced anthocyanin content, while, NO donor SNP (Sodium Nitroprusside) addition considerably reversed this inhibition and by contrast, resulted in a significant increase in anthocyanin accumulation, closely paralleling the transcripts of structural genes and transcription factors. Moreover, NO content, NR (nitrate reductase) activity and expression level of NOA (nitric oxide associated factor) were up-regulated by Hemin. Conclusions:Those consequences indicated that NO might work downstream in Hemin-heightened anthocyanin accumulation in radish sprouts.
Functional Gene Assessment of Wheat: Breeding Implication in Ningxia Province
Authors: Zhang Weijun , Zhao Junjie , He Jinshang , Kang Ling , Wang Xiaoliang , Zhang Fuguo , Hao Chenyang , Ma Xiongfeng , Chen Dongsheng 
Published: December 2020

Abstract
Background: The overall genetic distribution and divergences for cloned genes among wheat varieties that occurred during the breeding process of the past few decades in Ningxia province of China are poorly understood. Here we report the genetic diversities of 44 important genes underpinning grain yield, quality, adaptation and resistance in 121 Ningxia and 86 introduced wheat cultivars and advanced lines.Results: Population structure indicated characteristics of genetic components of Ningxia wheats including landraces of particular genetic resources, introduced varieties with rich genetic diversities and modern cultivars in different times. Analysis of allele frequencies showed that dwarfing alleles Rht-B1b at Rht-B1 and Rht-D1b at Rht-D1 , 1BL/1RS translocation, Hap-1 at GW2-6B and Hap-H at Sus2-2B are present very frequently in Ningxia modern cultivars and introduced varieties from other regions, but absent in landraces, indicating that the introduced wheat germplasm with numerous beneficial genes are vital for broadening genetic diversities of Ningxia wheat varieties. Large population differentiation occurred at adaptation genes between modern cultivars and well-adapted landraces. Founder parents have excellent allele combinations of important genes with a higher number of favorable alleles compared with modern cultivars. The gene flows manifested that six founder parents greatly contributed to breeding improvement in Ningxia province, in particular Zhou 8425B for yield related genes.Conclusions: These results will greatly benefit for wheat breeding in Ningxia province and other areas with similar ecological environments.
Comparative Transcriptome Analysis of Genes Involved in Penicillium Chrysogenum Induced Resistance to Root-knot Nematode in Tomato&nbsp;
Authors: Zhang Mengyue , Sikandar Aatika , Zhu Xiaofeng , Wang Yuanyuan , Liu Xiaoyu , Fan Haiyan , Xuan Yuanhu , Chen Lijie , Duan Yuxi 
Published: December 2020

Abstract
Background: Tomato root-knot nematode is a soil-borne disease caused by Meloidogyne incognita. Enhancement of natural plant-defense mechanisms to provide resistance against pathogens may be a promising alternative environmentally friendly nematode management approach. Recently, the biocontrol effect against different pathogens in the presence of Penicillium chrysogenum has been reported in a wide range of plants and pathogens. For understanding the molecular mechanisms of the resistance induced by P. chrysogenum Snef1216 to RKN, transcriptomes of inducer control ‘IRCK’ (induced by Snef1216 only) and pathogen + inducer ‘IRN’ were compared to those of control groups, namely negative control ‘CKCK’ (no inoculum), pathogen control ‘CKN’ (inoculum of RKN only).Results: Numerous high-quality reads were generated by Novogene, means of the RNA-seq method. After being aligned to the reference genome, four comparative transcriptomic profile maps between any pairwise comparisons were obtained to find significantly differentially expressed genes (DEGs) and three databases of induced resistance (IR)-related, nematode pathogenesis (NP)-related and basal resistance (BR)-related genes were gotten. By hierarchal clustering, the phylogenetic relationship between highly and fewer DGEs were obtained and classified the resistance and susceptible responses into two clusters after inoculation with RKN. Finally, the results were verified by RT-qPCR and analysis of important plant defense enzymes.Conclusions: Within an integrated and more sustainable management approach, the use of biocontrol organisms, like P. chrysogenum, seems to be a promising alternative.
Time-of-day Dependent Light Effects on Subjective Affect and Comfort
Authors: Chen Lijun , Yan Fangfang , Fan Shuhan , Wu Yifan , Yang Jia , Yang Hua , Huang Chang-Bing 
Published: December 2020

Abstract
Light, one of the key environmental components for both life and work, played significant role in subjective feelings (e.g. affect and comfort), but the exact effects and mechanisms were still to be determined. The present study screened thirty healthy adults (13 females, 22.45 ± 3.26 years) and examined subjective affect and comfort under short-term white lights with different combination of correlated color temperature (CCT) and illuminance at different times of day (e.g. morning, afternoon, and evening). Our results showed a significant interaction between illuminance level and time-of-day on subjective comfort. Participants felt more comfortable under 50 lx and 100 lx instead of 500 lx in the evening, and more comfortable under 500 lx in the morning and afternoon. In addition, a positive correlation between illuminance and comfort in the morning and a negative correlation between them in the evening were found. No significant effect of CCT on any subjective feeling was revealed. Our results necessitate the consideration of time-of-day in understanding lighting effects and application of healthy lighting in daily life.
Improper Acupuncture Causing Severe Multiple Soft Tissue Damage: A Case Report
Authors: xie yulong , peng yan , zhou wengzong , qin qiantong , wang hui , ni bing , wang wenjie , li tianyu , liu yanqing , jiang donghong , dong yunxia , miao rui 
Published: December 2020

Abstract
Background Acupuncture therapy is well known. But there is no report that acupuncture in patients with coagulation dysfunction in stroke may lead to severe soft tissue injury. we report a case of acupuncture leading to severe soft tissue injury.Methods A 63-year-old female patient was hospitalized for stroke rehabilitation. After 2 years of discharge, severe subcutaneous hematoma caused by acupuncture was readmitted to hospital. She received medication and physical rehabilitation. we monitored the international standardized ratio (INR) in plasma and observed the degree of dissipation of subcutaneous hematoma. the changes of patients during treatment were explored by data analysis.Findings Subcutaneous hematoma (area 20×20 cm) disappeared completely after 3 weeks of physical factor therapy. Small range (area ≤3×3 cm) subcutaneous hematoma can heal itself within 2 weeks. Improper acupuncture is only one of the causes of soft tissue injury. Stroke with coagulation dysfunction is not a contraindication of acupuncture treatment.Interpretation Our report suggests that previously unanticipated acupuncture treatment for stroke patients with coagulation dysfunction may lead to severe multiple soft tissue injuries. Although coagulation dysfunction is not a contraindication of acupuncture treatment, acupuncture treatment for stroke patients with coagulation dysfunction should be vigilant, strengthen the level of acupuncture operation, and fundamentally eliminate the problem. These cases are validation and supplement of adverse clinical reactions caused by improper acupuncture.
Rubella Virus-associated Uveitis: The Essentiality of Aqueous Humor Virological Analysis
Authors: Provost Julien , Labetoulle Marc , Bouthry Elise , Haigh Oscar , Leleu Igor , Kobal Alfred , Mouriaux Frédéric , Barreau Emmanuel , Vauloup-Fellous Christelle , Rousseau Antoine 
Published: December 2020

Abstract
Aims / background: Rubella virus-associated uveitis (RVAU) classically presents with the clinical features of Fuchs uveitis syndrome (FUS). We report a series RVAU, and discuss the relevance of available diagnostic strategies, and vaccination to prevent disease. Methods: We retrospectively included patients with RV-positive aqueous humor (AH) with RT-PCR and/or intraocular RV-IgG production, between January 2014 and December 2019. RV-IgG titers from AH and serum were compared with other virus-specific IgG titers (VZV and/or CMV and/or HSV-1), to determine the derived Goldmann-Witmer coefficient (GWC’). Clinical findings at presentation and during follow-up are reported, as well as the anti-RV vaccination status.Results: All 13 included patients demonstrated intraocular synthesis of RV-IgG (median GWC’: 9.5; 3.2-100). RV-RNA was detected in one patient while PCR results were negative for other HSV1, VZV and CMV. The mean delay in diagnosis was 13 ± 12.6 years, with an initial presentation of FUS in only 3 patients (23%). Only four patients had been vaccinated, but all after the recommended age. Conclusion: As RVAU is a pleiomorphic entity, virological analysis (RV RT-PCR and GWC’) of aqueous humor is essential to improve the diagnosis and management of this entity. Improper vaccination against RV appears to be implicated in RVAU.
Construction of an Effective Delivery System for DNA&nbsp;Vaccines Using Biodegradable Polylactic Acid Based Microspheres
Authors: Ren Shuaikai , Guo Liang , Wang Chunxin , Ru Jiaxi , Yang Yunqi , Wang Yan , Sun Changjiao , Cui Haixin , Zhao Xiang , Guo Huichen 
Published: December 2020

Abstract
Background: Nanotechnology represents a new impetus for biomedical research applications, especially using nanotechnology to formulate microspheres or nanospheres based delivery system for treatment of infectious diseases in animals.Results: Polylactic acid (PLA) microspheres with an average size of 156nm were prepared by combining emulsion polymerization coupled with emulsion−solvent evaporation. Coating with three different molecular weights of polyethylenimine (PEI) polymers increased the surface charges of the resulting PLA/PEI microspheres. Electrostatic interactions enabled plasmid DNA to adsorb tightly to the microspheres. After pig kidney-15 cells were cultured with the PLA/PEI/DNA complexes for 48 h, all three PLA/PEI microspheres successfully transferred plasmid DNA into the cells with high transfection efficiency. The protection rate of PLA/PEI microspheres loaded with DNA vaccine against foot-and-mouth disease in guinea pigs reached 87.5%, which was significantly higher than that of the control group injected with only DNA vaccine.Conclusions: In this work, PLA/PEI microspheres were constructed by preparing PLA microspheres and modifying with PEI polymers, and were shown a great potential in improve the efficacy, biosafety and economic effects of DNA vaccines. The results indicated PLA/PEI microspheres were expected to be an effective delivery system for DNA vaccines.
Comparison of Various Orgainc Acids for xylo-oligosaccharide Productions in Term of pKa Values and Combined Severity
Authors: Cao Rou , Liu Xinlu , Guo Jianming , Xu Yong 
Published: December 2020

Abstract
Background: Methods to produce XOS have been intensively investigated, including enzymatic hydrolysis, steam explosion, and acid hydrolysis. Acid hydrolysis is currently the most widely used method to produce XOS due to its advantages of fewer processing steps, stronger raw material adaptability, higher yield, and better reproducibility. Especially, organic acids such as acetic acid, formic acid and xylonic acid work better as compared with mineral acids. However, the catalytic mechanism of different organic acids has been little studied. In this paper, four different organic acids, including formic acid, glycolic acid, lactic acid, and acetic acid were selected to compare their hydrolytic effects.Results: Using pKa values as the benchmark, the yield of XOS increased with the increasing value of pKa. The yield of XOS was 37% when hydrolyzed by 5% acetic acid (pKa=4.75) at 170℃ for 20 min. Combined severity (CS), a parameter associated with temperature and reaction time was proposed, was proposed to evaluate the hydrolysis effect. The results of CS were consistent with that of pKa values on both the yield of XOS and the inhibitor.Conclusion: The results based on pKa values and combined severity, a parameter associated with temperature and reaction time, concluded that acetic acid is a preferred catalyst. Combining the techno-economic analysis and environmental benefits, acetic acid hydrolysis process has lower factory production costs, and it is also an important metabolite and a carbon source for wastewater anaerobic biological treatment. In conclusion, production of xylo-oligosaccharides by acetic acid is an inexpensive, environment-friendly, and sustainable processing technique.
Polyploidization, hybridization, and maternal and paternal lineages in Cyprinids (Teleostei: Cypriniformes)
Authors: Wen Yan ling , Chai Jing , Ma Wei , Murphy Robert W. , He Shunping , Chen Ziming , Zhang Ya-ping , Lu Xuemei 
Published: December 2020

Abstract
Background

The reconstruction of phylogenetic relationships for allopolyploids using genomic data is challenging because hybridization and polyploidy can blur history. In cyprinids, one allopolyploidization event involves goldfish and common carp, yet little is known about the origins of other cyprinid polyploid lineages, including their maternal and paternal ancestral lineages.
Results

Herein we employ 10 HOX genes, genomic and transcriptomic data of representative species from seven subfamilies in Cyprinidae to investigate the origins of polyploid lineages. Analyses of the nuclear and mitochondria genomes reveal that the Schizothoracinae and Cyprininae share the same maternal common ancestor, and identify the candidate genes for identifying maternal gene-copies from Cyprininae. Gene-trees show a close relationship between Sinocyclocheilus grahami and Cyprininae, and indicate that they share the same whole genome duplication (WGD) event about 12.10 ~ 14.03 Ma. Another allopolyploidization event involves Torinae; one duplication clustering with Schizothoracinae and Schizopygopsinae identifies them as paternal siblings. Further, Labeoninae has a history of recurrent hybridization, which is supported by both gene-trees from genomes and HOX genes.
Conclusions

Collectively, the diverse WGD history makes Cyprinidae a candidate system for investigating the origin of hybridization and polyploidization in vertebrates. Further investigations should enlarge the representatives, combine morphological traits, and reconstruct gene-trees based on whole-genome markers.