bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical Significance of CRP and IL-6 Levels During the Perioperative Period of Hip Arthroplasty for Aged Patients with Femoral Neck Fractures
Authors: Zhao Fulong , Guo Lijuan , wang xuefei , Zhang Yakui 
Published: May 2020

Abstract
Background Aged patients with femoral neck fractures underwent hip arthroplasty become prone to infection. And serum CRP and IL-6 might be sensitive indicators of inflammation. The aim of the study is to analyze and explore the clinical significance of changes in C-reactive protein (CRP) and interleukin-6 (IL-6) levels during the perioperative period in older patients that underwent hip arthroplasty for femoral neck fractures.Methods This retrospective study analyzed data for 82 patients that underwent hip arthroplasty for femoral neck fractures from June 2018 to June 2019in our hospital. Of these, 39 patients underwent hemiarthroplasty (HA) and 43 underwent total hip arthroplasty (THA). CRP and IL-6 were measured before the operation (baseline) and on the 1st, 2nd, 3rd, and 7th day after hip arthroplasty. Comparisons between groups were performed with the t test and χ2 test.Results Post-operative serum CRP and IL-6levels showed a trend of first increasing, then decreasing, but all levels were higher than baseline. The serum CRP level peaked on the third post-operative day, and IL-6 peaked on the first post-operative day. We found no significant difference in CRP or IL-6 levels between patients that received HAs and patients that received THAs.Conclusion We showed a trend in CRP and IL-6 changes during the perioperative period. These findings suggested that CRP and IL-6 might be involved in the early inflammatory response. Thus, both CRP and IL-6 levels might be useful for evaluating the postoperative inflammatory response in older patients with femoral neck fractures that undergo hip arthroplasty.
Histone demethylase JMJD3 disrupts spectrin-dependent cytoskeleton in Pancreatic Ductal Adenocarcinoma cells by regulating H exokinase domain containing 1 expression
Authors: Xiao Zhangang , Shen Jing , Zhao Qijie , Xiang Shixin , Zhu Yinxin , Xiao Mingtao , Li Chi Han , Cho Chi Hin , Tong Joanna Hung-Man , To Ka-Fai , Chen Yangchao 
Published: May 2020

Abstract
Background: JMJD3 is a jmjd domain containing histone demethylase which can remove methyl groups from lysine 27 of histone 3 (H3K27) to active histone methylated genes. Previous studies have demonstrated that JMJD3 played a crucial role in inflammation. Methods: Our study showed that JMJD3 was significantly down-regulated in pancreatic ductal adenocarcinoma (PDAC) cell lines and tissues. Restored expression of JMJD3 inhibited oncogenic phenotypes of PDAC cells, including cell proliferation, cell migration, and in vivo tumorigenicity, indicating a tumor suppressive role. Gene-expression microarray revealed that Hexokinase domain containing 1 (HKDC1) was one of the JMJD3 downstream targets. Results: The expression of JMJD3 and HKDC1 in PDAC tissues was positively correlated. High H3K27 tri-methylation (H3K27me3) status in HKDC1 gene was attenuated by ectopic expression of JMJD3 in PDAC cells, suggested that JMJD3 regulated HKDC1 expression by histone demethylation activity. The tumor suppressive role of HKDC1 in PDAC was also proved. Moreover, HKDC1 was demonstrated to competitively bind to spectrin beta Ⅱ to induce cytoskeleton disruption, which may contribute to tumor suppression. Conclusion: Taken together, our study indicates that JMJD3 may disrupt spectrin-dependent cytoskeleton via activation of HKDC1 to suppress PDAC.
Possibility of Live Birth For Patients With Low Serum β-hCG 14 Days After Blastocyst Transfer
Authors: Wu Yixuan , Liu Haiying 
Published: May 2020

Abstract
Background: Although many previous study investigated the prediction of pregnancy outcomes by serumβ-hCG levels after blastocyst transfer, no study focused on the pregnancy outcomes of patients with initially low serum β-hCG levels. The purpose of the study was to investigate the pregnancy outcomes of patients with low serum β- level 14 days after blastocyst transfer. Methods: A retrospective study was performed in the Third Affiliated Hospital of Guangzhou Medical University. The purpose was to investigate the patients whose serum β-hCG levels were between 5-299 mIU/ml 14 days after frozen blastocyst transfer. Rates of live birth, early miscarriage, biochemical pregnancy loss and ectopic pregnancy were analyzed according to the female patients’ age by chi-squared test. Receiver operating characteristic (ROC) curves were plotted to explore the threshold for prediction of clinical pregnancy and live birth. Results: A total of 312 patients had serum β-hCG levels <300 mIU/ml 14 days after frozen blastocyst transfer, among which 18.6% were live birth, 47.4% were early miscarriage, 22.8% were biochemical pregnancy and 9.6% were ectopic pregnancy. Pregnancy outcomes were comparable between the patients aged <38 years and ≥38 years. ROC curve analysis showed that the predicted value ofβ-hCG for clinical pregnancy was 58.8 mIU/ml with the AUC of 0.752(95%CI :0.680-0.823), sensitivity of 95.0% and specificity of 53.5%.The threshold for live birth was 108.6mIU/ml with the area under the ROC curve (AUC) of 0.649(95%CI:0.0.583-0.715), sensitivity of 93.1% and specificity of 37.0%.For the β-hCG fold increase over 48 hours, the cut-off for clinical pregnancy was 1.4 with the AUC of 0.899(95%CI :0.801-0.996), sensitivity of 90.3% and specificity of 77.8%; the threshold for live birth was 1.9 with the AUC of 0.808(95%CI :0.724-0.891), sensitivity of 88.5% and specificity of 64.5%. Conclusions: Initially low serum β-hCG level 14 days after frozen blastocyst transfer indicated minimal likelihood of live birth. For patients having initial β-hCG >58.8 mIU/ml, luteal phase support is suggested to continue. Another serum β-hCG test and ultrasound should be performed one week later. If the initial serum β-hCG is < 58.8 mIU/ml, luteal phase support is suggested to discontinue and measurement of serum β-hCG and ultrasound can be arranged one week later.
Prevalence of Tissue BRCA Gene Mutation in Ovarian, Fallopian Tube and Primary Peritoneal Cancer: Thai Multi-institutional Study
Authors: Lertkhachonsuk Arb-aroon , Suprasert Prapaporn , Manchana Tarinee , Kittisiam Thannaporn , Kantathavorn Nuttavut , Chansoon Tharintorn , Khunamornpong Surapan , Pohthipornthawat Natkrita , Tangjitgamol Siriwan , Luasiripanthu Taksa , Teerapakpinyo Chinachote , Shuangshoti Shanop , Iemwimangsa Nareenart , Chantratita Wasun 
Published: May 2020

Abstract
Background:Ovarian, fallopian tube or primary peritoneal cancer patientswithBRCA gene mutation have enhanced sensitivity to platinum-based regimens and PARP inhibitors. However, the knowledgeregardingBRCA mutation in Thai patients are limited. This study is to identify the prevalence and characteristics of somatic and germline BRCA1&2mutations in Thai patients with these cancers.Methods::The paraffin blocks of tumorsthat had histology of high grade serous or high grade endometrioid, or clear cell carcinomawhich obtained between June 2016 and December 2017 were processed to evaluate BRCA mutation using next-generation sequencingsystem. Blood or normal tissue paraffin blocks of positive patients were further tested for germline BRCA mutation. Results:Tissue paraffin blocks of 178 patients were collected. Only 139 patients whose paraffin-blocks could be processed. PositiveBRCAmutation was identified in 24 cases (17.3%): BRCA1in 13 cases, BRCA2 in 10 cases, and BRCA1&2in another. Germline mutation study in blood or normal tissue in 23positive patients revealed BRCA mutation in 14 cases: BRCA1(8 cases)and BRCA2 (6 cases). Overall, the prevalence of somatic and germline mutation were 6.5% (9 in 138 patients) and 8.7% (14 in 138 patients) respectively. The most common histology associated withBRCA mutation was high grade serous cancer (27.3%). No significant differencebetween,stage, outcome, platinum status and survival outcome in patients with or without BRCA mutation.Conclusion: BRCA mutation in Thai ovarian cancer patients was demonstrated in less than 10%. Higher rate of mutation was found in high grade serous cancer.
Baked Food Consumption Is A Risk Factor for Chronic Kidney Disease: A 1:1 Paired Case-control Study
Authors: Lai Xiaoying , Zhou Shiyu , Lin Zewei , Yan Chuanjie , Zhu Hong , Li Wenyuan , An Shengli , Ouyang Ping 
Published: May 2020

Abstract
Background

Chronic Kidney Disease (CKD) has become a global public health problem. Identifying the risk factors of CKD can provide strategies for the prevention of CKD. Studies showed that lifestyles play an important role in CKD, but the relationship between them remained unclear. Thus we aimed to explore the association of lifestyle behaviors (the dietary habits especially) with CKD.
Methods

A 1:1 matched case-control study including 1414 participates from the HUIQIAO health database system from Jan. 2015 to Dec. 2018 was conducted. Our main outcome measure was the diagnose of CKD, and exposures were lifestyle behaviors measured by a questionnaire. The anthropometric characteristics were included as confounding variables.
Results

We used conditional logistics regression to assess the odds ratios (ORs) and adjusted ORs for the risk factors. With the assumption of missing at random (MAR) pattern, the missing values of confounding variables and exposures were handled by multiple imputation. We found that lifestyle behaviors regarding skipping breakfast ≥ 3 times per week (1.672, 95% CI, 1.086–2.574), sleep efficiency ≤ 75% (1.633, 95% CI, 1.195–2.232), consuming baked food frequently (1.683, 95% CI, 1.163–2.434), proper intake of oil (0.789, 95% CI, 0.624–0.996), proper intake of aquatic product (0.732, 95% CI, 0.567–0.944), proper intake of soybean and nuts (0.625, 95% CI, 0.447–0.873) were associated with CKD.
Conclusion

These results suggest that moderate intake of soybean, nuts, and aquatic product, control oil intake, having breakfast at least 4 times per week, maintaining good sleep efficiency, and consuming less baked foods may contribute to prevent the development of CKD.
Trial registration:
This study is registered at http://www.chictr.org.cn (ChiCTR2000031864).
Relationships between motor scores and cognitive functioning in FMR1 female premutation X carriers indicate early involvement of cerebello-cerebral pathways.
Authors: Storey Elsdon , Bui Minh Q , Stimpson Paige , Tassone Flora , Atkinson Anna , Loesch Danuta 
Published: May 2020

Abstract
Background Smaller expansions of CGG trinucleotide repeats in the FMR1 X-linked gene termed ‘premutation’ lead to a neurodegenerative disorder: Fragile X Associated Tremor/Ataxia Syndrome (FXTAS) in nearly half of aged carrier males, and 8-16% females. Core features include intention tremor, ataxia, and cognitive decline, and white matter lesions especially in cerebellar and periventricular locations. A ‘toxic’ role of elevated and expanded FMR1 mRNA has been linked to the pathogenesis of this disorder. The emerging issue concerns the trajectory of the neurodegenerative changes: is the pathogenetic effect confined to overt clinical manifestations? Here we explore the relationships between motor and cognitive scale scores in a sample of 57 asymptomatic adult female premutation carriers of broad age range. Methods Three motor scale scores (ICARS-for tremor/ataxia, UPDRS-for parkinsonism, and the Clinical Tremor) were related to 11 cognitive tests and two psychiatric pathology scores - DASS and SCL-90 - using Spearman’s rank correlations. Robust regression, applied in relationships between all phenotypic measures, and genetic molecular and demographic data, identified age and educational levels as common correlates of these measures, which were incorporated as confounders in correlation analysis. Results Cognitive tests demonstrating significant correlations with motor scores were those assessing psychomotor speed/visual attention (SDMT; TMT-A) and those dependent on various aspects of executive functioning for their execution: sequencing and alternation (TMTB-A); working memory (DS backwards); and non-verbal reasoning (MR). The largest number of motor x cognitive correlations involved ICARS and Tremor scales, which reflect the type of motor dysfunctions seen in FXTAS. Conclusions Subtle motor impairments correlating with cognitive deficits may occur in female premutation carriers not meeting diagnostic criteria for FXTAS. This pattern of cognitive deficits is consistent with those seen in other cerebellar disorders. Our results provide evidence that more than one category of clinical manifestation reflecting cerebellar changes – motor and cognitive - may be simultaneously affected by premutation carriage across a broad age range in asymptomatic carriers. Future longitudinal studies should determine whether cognitive dysfunction tracking motor impairments is driven by the premutation status common to all carriers, or only to a subset of carriers who will develop FXTAS in older age.
Heat Dissipation Performance of Loader-Digger Cooling System based on Simulation and Test Data
Authors: Li Lei , Chen Xi , Liu Di , Zhou Hong-gen , Wu Haiyan 
Published: May 2020

Abstract
High temperature of the radiator group is harmful to the power system and hydraulic system. In order to improve the heat dissipation performance of the loader, the flow field characteristics of the cooling chamber are analyzed by simulation and heat balance test. Firstly, the mathematical model of heat flow is established. Secondly, the flow field in the cooling chamber under different speeds is simulated based on CFX. And then the influence of fan position and internal flow field distribution on radiator performance is studied. Through the simulation of four different distances, it is concluded that the optimal distance between cooling fan and radiator is 76 mm. Finally, a testing system is built for the temperature acquisition of engine water radiator, torque converter oil radiator, hydraulic oil radiator and air-to-air cooler of the hood structure, and then the simulation and optimization results are verified.
Inhibition of multiple SARS-CoV-2 proteins by an antiviral biomolecule, seselin from Aegle marmelos deciphered using molecular docking analysis
Authors: Nivetha R. , Bhuvaragavan S. , Janarthanan S. 
Published: May 2020

Abstract
Our earlier experimental and computational report produced the evidence on anti-viral nature of the compound seselin purified from the leaf extracts of Aegle marmelos against Bombyx mori Nuclear Polyhedrosis Virus (BmNPV). In the pandemic situation of COVID-19 caused by SARS-COV-2 virus, an in silico effort to evaluate the potentiality of the seselin has been made to test its efficacy against multiple targets of SARS-COV-2 such as SARS-CoV-2S spike protein, COVID-19 main protease and free enzyme of the SARS-CoV-2 (2019-nCoV) main protease. The ligand, seselin showed the best interaction with receptors SARS-CoV-2S protein, COVID-19 main protease and free enzyme of the SARS-CoV-2 (2019-nCoV) main protease with a binding energy of -6.6 kcal/mol, -6.9 kcal/mol and -6.7 kcal/mol, respectively. Docking analysis with three different receptors identified that all the computationally predicted lowest energy complexes were stabilized by intermolecular hydrogen bonds and stacking interactions. The aminoacid residues involved in interactions are THR111 and GLN110 for spike protein, SER1003, ALA958 and THR961 for COVID-19 main protease, and for SARS-CoV-2 (2019-nCoV) main protease, it is THR111, GLN110 and THR292. The outcome of pharmacokinetic analysis suggests that the compound had favourable drugability properties by obeying Lipinski rule of five with efficient ADME properties and exhibiting high affinity towards the binding site that it was directed to. The results suggest that the seselin has inhibitory potential over multiple SARS-COV-2 targets and holds a high potential to work effectively as a novel drug for COVID-19, if evaluated in experimental set ups in foreseeable future.
Prognostic Impact of Coagulopathy in Patients with COVID-19: a Meta-analysis of 35 Studies and 6427 Patients
Authors: Polimeni Alberto , Leo Isabella , Spaccarotella Carmen , Mongiardo Annalisa , Sorrentino Sabato , Sabatino Jolanda , De Rosa Salvatore , Indolfi Ciro 
Published: May 2020

Abstract
Coronavirus Disease 2019 (COVID-19) is a highly contagious disease that appeared in China in December 2019. Several patients with severe COVID-19 infection can develop a coagulopathy according to the ISTH-criteria for disseminated intravascular coagulopathy (DIC). We conducted a meta-analysis of all available studies on COVID-19 to explore the impact of coagulopathy on severe illness and mortality. An electronic search was performed within PubMed, Google Scholar and Scopus electronic databases. The primary endpoint was the difference of D-dimer values between Non-Severe vs Severe disease and Survivors vs Non-Survivors. The primary analysis showed that mean d-dimer is significantly higher in COVID-19 patients with severe disease than in those without (SMD -2.15 [-2.73 to -1.56], I2 98%, P <0.0001). Additional analysis of platelet count showed lower levels of mean PLT in Severe patients than those observed in the Non-Severe patients (SMD 0.77 [0.32 to 1.22], I2 96%, P <0.001). Interestingly, longer mean PT was found in Severe group (SMD -1.34 [-2.06 to -0.62], I2 98%, P <0.0002) compared to Non-Severe group. In conclusion, the results of the present meta-analysis, the largest and most comprehensive to date, demonstrate that Severe COVID-19 infection is associated with higher D-dimer values, lower platelet count and prolonged PT.
Thermal state of the upper mantle and the origin of the Cambrian-Ordovician ophiolite pulse: Constraints from ultramafic dikes of the Hayachine-Miyamori ophiolite
Authors: Kimura Takafumi , Ozawa Kazuhito , Kuritani Takeshi , Iizuka Tsuyoshi , Nakagawa Mitsuhiro 
Published: May 2020

Abstract
Ophiolite pulses, which are periods of enhanced ophiolite generation and emplacement, are thought to have a relevance to highly active superplumes (superplume model). However, the Cambrian-Ordovician pulse has two critical geological features that cannot be explained by such a superplume model: predominance of subduction-related ophiolites and scarcity of plume-related magma activities. We addressed this issue by estimating the mechanism and condition of magma generation, including mantle potential temperature (MPT), from a ~500 Ma subduction-related ophiolite, the Hayachine-Miyamori ophiolite. We developed a novel method to overcome difficulties in global MPT estimation from an arc environment by using porphyritic ultramafic dikes showing flow differentiation, which have records of the chemical composition of the primitive magma, including its water content, because of their high pressure (~0.6 GPa) intrusion and rapid solidification. The solidus conditions for the primary magmas are estimated to be ~1450 °C, ~5.3 GPa. Geochemical data of the dikes show passive upwelling of a depleted mantle source in the garnet stability field without a strong influence of slab-derived fluids. These results, combined with the extensive fluxed melting of the mantle wedge prior to the dike formation, indicate sudden changes of the melting environment, its mechanism, and the mantle source from extensive fluxed melting of the mantle wedge to decompressional melting of the sub-slab mantle, which has been most plausibly triggered by a slab breakoff. The estimated MPT of the sub-slab mantle is ~1350 °C, which is very close to that of the current upper mantle and may reflect the global value of the upper mantle at ~500 Ma if small-scale convection maintained the shallow sub-slab mantle at a steady thermal state. We, therefore, conclude that the Cambrian-Ordovician ophiolite pulse is not attributable to the high temperature of the upper mantle. Frequent occurrence of slab breakoff, which is suggested by our geochemical compilation of Cambrian-Ordovician ophiolites, and subduction termination, which is probably related to the assembly of the Gondwana supercontinent, may be responsible for the ophiolite pulse.
Penerapan Perilaku Keluarga Sadar Gizi (KADARZI) dan Status Gizi Balita Usia 24-59 Bulan di Puskesmas Kranji Bekasi
Authors: Oktaviani Putri Pratiwi , Djafar Meylina , Fayasari Adhila 
Published: May 2020

<em>This study aimed to analyze association between the implementation of nutrition conscious family with the nutritional status of children aged 24-59 months in Puskesmas Kranji Bekasi. This research used a cross sectional design. Subjects were 71 mothers who had children aged 24-59 months taken by purposive sampling. Weight and height data were measured by stepping scales and microtoise, then converted to z-scores. Bivariate analysis using Chi-Square test. Underweight about 9 (12,7%) subjects, and 42 (59,2%) families did not apply KADARZI, indicator points that have not been done includes providing nutritional supplements (vitamin A) (7 %), varied meals (14,1%), using iodized salt (16,9%), weighing regularly (19,7%), and giving exclusive breastfeeding 31 (43,7%). Based on Chi-Square test is proved that there is correlation between KADARZI implementation with nutritional status of children aged 24-59 months. The implementation of KADARZI had a significant association between nutritional status of children aged 24-59 months (p-value 0,006), particularly at indicators irregularly posyandu participation.</em>
Physical Inactivity is Liable to the Increased Cardiovascular Risk and Impaired Cognitive Profile
Authors: Teixeira Robson B. , dos Santos Amorim Paulo Roberto , Marins João C.B. , de L.X. Martins Yuri , de Souza Magalhães Marques Samuel , Aguiar Victor P.R. , Palotás András , Lima Luciana M. 
Published: May 2020

Background:Sedentary life-style is a significant public health issue. It increases the incidence of type-2 diabetes mellitus (DM2) and systemic arterial hypertension (SAH), which in turn may impair physical and mental health. In fact, disrupted glucose metabolism is characteristic of Alzheimer’s dementia, and it is often dubbed as type-3 diabetes.Objective:The purpose of this study was to assess the level of activity, body composition, cardiovascular risk and cognitive profile of patients with DM2 and/or SAH. The study was cross-sectional design.Methods:The sample consisted of 120 individuals which 35% men and 65% women, with an average of 64±9 years old and 60±11 years old, respectively. Various parameters were evaluated such as anthropometric variables, pedometer recordings and brief cognitive screening battery (BCSB), which assesses the immediate memory, verbal fluency, learning, late memory and recognition. Chi-square and Fisher's exact test were applied to observe possible differences between men and women. In addition to Kruskall-Wallis, in the comparison between patients with SAH; DM2 and SAH + DM2.Results:A high rate of physical inactivity was found among those enrolled in this project. Females were characterized by increased body fat, whereas men displayed visceral fat excess. BCSB demonstrated reduced verbal fluency, late memory and recognition, with women presenting significantly worse results.Conclusion:Low level of daily physical activity is apparently correlated with obesity, elevated cardiovascular risk, and cognitive dysfunction.