bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sero - Prevalence and Risk Factors of Cattle and Human Brucellosis at Seka Chokorsa and Shebe Sonbo Jimma Zone Districts, South West Ethiopia
Authors: Tokon Ftsum Assefa , Gelalcha Benti Deresa , Moti Teferi Benti , Alemu Redeat Belaneh , Awash Hailu Degefu 
Published: November 2019

Abstract
Background: Brucellosis is contagious bacterial disease of major socio-economic and public health importance globally and it is also one of the priority zoonotic diseases in Ethiopia. Across-sectional epidemiological study was carried out from April 2017 to April 2018 to estimate the sero prevalence of bovine and human brucellosis and to assess the associated risk factors of brucellosis in Seka Chokorsa and Shebe Sonbo districts of Jimma zone. Results: The overall prevalence of cattle brucella infection at individual and herd level were 5.9% (95%CI: 4.1%-8.1%) and 26.6 (95%CI: 19.1%-35.3%) based on diagnosis using commercial kits of the competitive enzyme-linked Immunosorbent assay (CELISA) from Brucella abortus antibodies. Univariate logistic regression analysis at individual animal level showed that animals from large herd size and households which had a practice of introduction of new animals in their herds were 3.7 and 2.3 times more likely to be seropositive, respectively. The same scenario has been observed at herd level. Molecularly, five Brucella abortus was identified as the species affecting cattle in the study areas. From the total human serum samples tested only one serum was found to be positive for CFT and thus the prevalence is 0.42 %,( 95%CI: 0.01%-2.38%). From 238 respondents, 90% of them drink raw milk and milk products, and 70% of them also eat raw meat. Furthermore, slaughtering, assisting during delivery and poor management of aborted material is a common practice that favor transmission of the pathogen in the area. Conclusions: In conclusion, the results of this study showed that brucellosis is an important and widely distributed disease in cattle in the study areas. The finding of the infection in human indicates the public health importance of brucellosis in the area. The risk behaviors and practices observed suggests the need to design all-inclusive health programmes, such one-health approach aimed at controlling brucellosis spread in the study area.
CFTR deficiency causes cardiac dysplasia during zebrafish embryogenesis and is associated with dilated cardiomyopathy
Authors: Liu Yanyan , Lin Ziyuan , Liu Mingfeng , Liao Huijuan , Chen Yan , Zhang Xiaohu , Chan Hsiao Chang , Zhou Bin , Rao Li , Sun Huaqin 
Published: November 2019

Abstract
Mutations in the CFTR gene cause cystic fibrosis with myocardial dysfunction. However, it remains unknown whether CF-related heart disease is a secondary effect of pulmonary disease, or an intrinsic primary defect in the CF heart. Here, we used a zebrafish model, which lacks lung tissue, to investigate the role of CFTR in cardiogenesis during embryonic development. Our findings demonstrate that a loss of CFTR impairs cardiac development from the cardiac progenitor stage of heart development, resulting in cardiac looping defects, a dilated atrium, pericardial edema, and a decrease in heart rate. Furthermore, we found that cardiac development is perturbed in wild type embryos treated with a gating speciﬁc Cftr channel inhibitor, CFTRinh-172, at the blastula stage of development, but not with treatment at later stages. Gene expression analysis of blastulas indicated that transcript levels, including mRNAs associated with cardiovascular diseases, were significantly altered in embryos derived from cftr mutants relative to controls. To evaluate the role of CFTR in human heart failure, we performed a genetic association study on individuals with dilated cardiomyopathy and found that CFTR containing I556V mutation, which causes a channel defect, is associated with the disease. Similar to well-studied channel-defective CFTR mutants, CFTR I556V mRNA failed to restore cardiac dysplasia in mutant embryos.The present study reveals an important role for the CFTR ion channel in regulating cardiac development during early embryogenesis, supporting the hypothesis that CF-related heart disease results from an intrinsic primary defect in the CF heart.
Integrated mRNA and miRNA transcriptome analysis reveals a regulatory network for tuber expansion in Chinese yam (Dioscorea opposita)
Authors: Zhou Yun-Yi , Luo Shuzhen , Hameed Saba , Xiao Dong , Zhan Jie , Wang Aiqing , He Longfei 
Published: November 2019

Abstract
Background
Yam tuber is a storage organ, derived from the modified stem. Tuber expansion is a complex process, and depends on the expressions of genes that can be influenced by environmental and endogenous factors. However, little is known about the regulatory mechanism of tuber expansion. In order to identify the genes and miRNAs involved in tuber expansion, we examined the mRNAs and small RNAs in Dioscorea opposita (Chinese yam) cv. Guihuai 16 tuber during its initiation and expansion stages.
Results
A total of 14238 differentially expressed genes in yam tuber at its expansion stage were identified by using RNA sequencing technology. Among them, 5723 genes were up-regulated, and 8515 genes were down-regulated. Functional analysis revealed the coordination of tuber plant involved in processes of cell events, metabolism, biosynthesis, and signal transduction pathways at transcriptional level, suggesting that these differentially expressed genes are somehow involved in response to tuber expansion, including CDPK, CaM, CDL, SAUR, DELLA, SuSy, and expansin. In addition, 541 transcription factor genes showed differential expression during the expansion stage at transcriptional level. MADS, bHLH, and GRAS were involved in cell differentiation, division, and expansion, which may relate to tuber expansion. Noteworthy, data analysis revealed that 22 known tuber miRNAs belong to 10 miRNA families, and 50 novel miRNAs were identified. The integrated analysis of miRNA-mRNA showed that 4 known miRNAs and 11 genes formed 14 miRNA-target mRNA pairs were co-expressed in expansion stage. miRNA160, miRNA396, miRNA535 and miRNA5021 may be involved in complex network to regulate cell division and differentiation in yam during its expansion stage.
Conclusion
The mRNA and miRNA datasets presented here identified a subset of candidate genes and miRNAs that are putatively associated with tuber expansion in yam, a hypothetical model of genetic regulatory network associated with tuber expansion in yam was put forward, which may provide a foundation for molecular regulatory mechanism researching on tuber expansion in Dioscorea species.
The value of miR-155 as a biomarker for the diagnosis and prognosis of lung cancer: a systematic review with meta-analysis
Authors: Shao Chuchu , Yang Fengming , Qin Zhiqiang , Jing Xinming , Shu Yongqian , Shen Hua 
Published: November 2019

Abstract
Background: Recently, a growing number of studies have reported the coorelation between miR-155 and the diagnosis and prognosis of lung cancer, but results of these researches were still controversial due to insufficient sample size. Thus, we carried out the systematic review and meta-analysis to figure out whether miR-155 could be a screening tool in the detection and prognosis of lung cancer.
Methods: A meta-analysis of 13 articles with 19 studies was performed by retrieving the PubMed, Embase and Web of Science. We screened all correlated literaters until December 1st, 2018. For the diagnosis analysis of miR-155 in lung cancer, sensitivity(SEN), specificity (SPE), positive likelihood ratio (PLR), negative likelihood ratio (NLR), diagnostic odds ratio (DOR) and area under the ROC curve (AUC) were pooled to evaluate the accuracy of miRNA-155 in the diagnosis of lung cancer. For the prognosis analysis of miR-155 in lung cancer, the pooled HRs and 95% CIs of miR-155 for overall survival/disease free survival/progression-free survival (OS/DFS/PFS) were calculated. In addition, Subgroup and meta-regression analyses were performed to distinguish the potential sources of heterogeneity between studies.
Results: For the diagnostic analysis of miR-155 in lung cancer, the pooled SEN and SPE were 0.82 (95% CI: 0.72-0.88) and 0.78 (95% CI: 0.71-0.84), respectively. Besides, the pooled PLR was 3.75 (95% CI: 2.76-5.10), NLR was 0.23 (95% CI: 0.15-0.37), DOR was 15.99 (95% CI: 8.11-31.52) and AUC was 0.87 (95% CI: 0.84-0.90), indicating a significant value of miR-155 in the lung cancer detection. For the prognostic analysis of miR-155 in lung cancer, up-regulated miRNA-155 expression was not significantly associated with a poor OS (pooled HR = 1.26, 95% CI: 0.66-2.40) or DFS/PFS (pooled HR = 1.28, 95% CI: 0.82-1.97).
Conclusions: The present meta-analysis demonstrated that miR-155 could be a potential biomarker for the detection of lung cancer but not an effective biomarker for predicting the outcomes of lung cancer. Furthermore, more well-designed researches with larger cohorts were warranted to confirm the value of miR-155 for the diagnosis and prognosis of lung cancer.
The Responses Of Antakya (Antioch) Churches To Cultural Shifts
Authors: Yilmaz Yonca , Zeren Mine Tanaç 
Published: November 2019

Antakya (Antioch), located in the southern region of Turkey, is one of the oldest settlements in the country. Its history dates back to the prehistoric times. It has been through countless invasions throughout its history. It has been dominated by various civilizations and has been the center of many religions. The city, which was founded by Alexander the Great in the Roman period, has many routes to nearly all directions as a result of its geographical location. Due to its context, this makes the city the point of convergence of cultures. After the Roman period, Byzantine and Arab-dominated city (AC 395 — AC 963), were exposed to constant war between the Christian and Muslim communities for the domination right to the city. Today in Antakya, although the majority of the population is Muslim and Christian, the Sunni Arabs, Sunni Turks, Shia Arabs, Assyrians, Catholics, Orthodox Christians, Protestant Arabs, Arabs, Armenians, Jewish people and other minority groups all live together in harmony, thus forming the dynamics of multicultural city structure. The name “Christian” was first coined in this historic city. Antakya also hosts the Church of Saint Peter, which is believed to be one of the earliest Christian houses of worship, making it extremely valuable for Christianism. Indigenous inhabitants of Antakya have lived in the same land since the foundation of Christianity. Today, 90 percent of the Christians are Orthodox, 10 percent are Protestants and other believers, where the population of Christians are decreasing. Bearing in mind the aforementioned history and context, a research was conducted on the Orthodox Church, Antakya Protestant Church and Vakıflı Armenian Church which all still exist to this day in the city. Purpose of the research is to evaluate the structure of the churches in regards to the following parameters;- The responses of the churches to the indigenous inhabitants- Cultural shifts in the ever-changing sociocultural values of the society- The city image they present.The reason behind choosing these three structures for the study is the fact that all three structures boast Christian symbolism and imagery.
Process of developing education material on water, sanitation and hygiene (WaSH) and diarrhoea prevention and testing its acceptability among tribal mothers
Authors: Jeyakumar Angeline , Godbharle Swapnil , Giri Bibek Raj , Mirzaie Zakir Hussain , Jori Chandrashekhar 
Published: November 2019

Abstract
The study aimed to develop education material on WaSH practices and assess its content and acceptability. Online survey of 19 posters identified two Marathi posters that were not context- or setting-specific. A three-phased evaluation study was conducted to assess (i) WaSH practices of mothers attending an ANC centre (n = 40) and (ii) performed a quantitative content analysis of education material developed on WaSH (n = 38). Focus group discussions (FGDs) were employed for qualitative content analysis (n = 10). (iii) Acceptability was derived from the scores of quantitative content analysis. Relevance of content and use of fear appeal emerged as significant characteristics using qualitative content analysis. In quantitative content analysis, the maximum score for the domains pertaining to relevance and comprehension was 5 each and for the layout was 3. The mean scores for posters 1 and 2 were 4.34 ± 0.9 and 4.00 ± 1.1 for relevance; and 4.89 ± 0.3 and 4.95 ± 0.2 for comprehension, respectively. Acceptability ranging between 68 and 100% in all three domains for both the posters indicated high acceptability. Maximum acceptability score was obtained for comprehension followed by relevance and layout for both the posters. Tribal populations can benefit from these communication aids that are context- and setting-specific. These can be adopted with relevant cultural modifications to suit similar settings.
Comparative performance of regression and the Markov based approach in the prediction of the future condition of a water distribution pipe network amidst data scarce situations: a case study of Kampala water, Uganda
Authors: Sempewo Jotham Ivan , Kyokaali Lydia 
Published: November 2019

AbstractGlobally, water utilities are grappling with the challenge of predicting the condition of deteriorating pipe network infrastructure amidst financial constraints and data-scarce scenarios. As a result, new innovative approaches such as statistical regression and Markov-based approaches have been introduced to aid water distribution pipe renewal decision making. However, comparison of the performance of these models under limited data has not been undertaken so far. In addition, the models have been applied elsewhere, in different environments and data availability scenarios. This paper addresses therefore the mentioned research gap and compares the performance of statistical regression and Markov models in the prediction of a condition of a pipe in a developing country. In addition, the criticality analysis of a block is studied. The data used for assessment is from Kampala water, the largest area in the National Water and Sewerage Corporation, Uganda. The results show that 78.26% of the prediction of the regression model is accurate in comparison to 88.4% for the Markov model. This means that the Markov-based approach is more superior than a regression model in a data scarce scenario. The approach will go a long way in helping water utilities in development of water decision pipe renewal plan amidst a limited budget and in data scarce scenarios.
El mercado laboral en el Caribe y las desigualdades socio-laborales (1890-1930) Los trabajadores calificados de la UFCO y de la Northern Railway Company a las puertas de la crisis de 1929
Authors: Sánchez Lovell Adriana 
Published: November 2019

En este escrito se hace referencia a las transformaciones en el mercado laboral en la Región Caribe de Costa Rica Costa Rica. El objetivo consiste en comprender cambios en la composición de la fuerza laboral y en las condiciones laborales, en lo que respecta a la producción agroexportadora así como a la construcción de infraestructura ferroviaria, en un periodo comprendido desde 1890 hasta 1930. Para establecer las conexiones, se discuten las relaciones comerciales entre Costa Rica, Panamá y Jamaica, las migraciones laborales entre las mismas, y las investigaciones sobre la United Fruit Company en Cuba, que además, son tomadas como referente comparativo. La bibliografía consultada, consiste en publicaciones relativas a la historia del trabajo de las multinacionales bananeras en Centroamérica y el Caribe ístmico e insultar. Las fuentes comprenden los Censos Nacionales de 1891 y 1927, así como el Fondo Northern, disponible en el Archivo Nacional de Costa Rica (ANCR).
Palabras Clave: historia del trabajo, mercado laboral, ferrocarriles, caribe.
Matemática y complejidad
Authors: Polanía Quiza Luis Arturo , Ovalle Cerquera Daniel Enrique , Cárdenas Lis Juan David 
Published: November 2019

En este trabajo se presentan algunos elementos relacionados con el enfoque o filosofía de la complejidad y su integración con la matemática. Se describen las particularidades de los sistemas dinámicos no-lineales como sistemas complejos, hecho raíz de la noción de ver a las matemáticas como una disciplina unificadora de la ciencia: una disciplina transdisciplinar. Además, se entiende el Teorema de Incompletud de Kurt Gödel, como una de las más significativas razones que puede argumentar la insuficiencia teórica de la lógica clásica a partir de fundamentos matemáticos, ajustándose a la noción de indecidibilidad de Murray Gell-Man, y así, abrir las puertas a las lógicas no clásicas, en particular, a la lógica del tercero incluido como la lógica de la complejidad.
Palabras Clave: Complejidad; Transdisciplina; Sistemas Dinámicos no-lineales; Lógica No-Clásica; Indecidibilidad; Sistemas Complejos
Evaluaciones de impacto social. Los antropólogos en la elaboración de proyectos de la Comisión Federal de Electricidad en México
Authors: Silvia Salazar Patiño Janeth , Arriaga Ornelas José Luis 
Published: November 2019

En México el servicio de energía eléctrica es prestado de manera casi-monopólica por la Comisión Federal de Electricidad (CFE), que es en una "empresa productiva del Estado" y presenta tendencias de crecimiento sostenidas, lo cual le lleva a emprender proyectos de infraestructura permanentemente. El nuevo marco normativo para realizar esos proyectos le obliga a realizar Evaluaciones de Impacto Social previas. En este trabajo se describe la inclusión de algunos antropólogos para colaborar en las primeras evaluaciones, se comparte la vivencia directa en algunos de ellos y se reflexiona sobre el tipo de rol que se le pide jugar. Se expone cómo se están llevando a cabo las evaluaciones y se ofrecen datos recabados durante proyectos de subestaciones y nuevas líneas de transmisión en la región de Silao, Guanajuato, en donde se documentó que la gente se resiste, ya sea por desconfianza o como estrategia para obtener la mejor ganancia. Finalmente se reflexiona sobre la posición del antropólogo, las limitaciones que ahora se le imponen en este tipo de estudios y las potencialidades que tendría como mediador y evaluador de los proyectos, sin soslayar la disyuntiva deontológica al colaborar en proyectos e influir en las decisiones que orienten las acciones planificadas de la CFE.

Palabras clave: Evaluación de impacto social; antropología; energía eléctrica; Comisión Federal de Electricidad.
In Vitro Investigation Demonstrates IGFR/VEGFR Receptor Cross Talk and Potential of Combined Inhibition in Pediatric Central Nervous System Atypical Teratoid Rhabdoid Tumors
Authors: Obaid Halah , Kannappan Sunand , Gupta Mehul , Ruan Yibing , Zhang Chunfen , Bose Pinaki , Narendran Aru 
Published: November 2019

Background:
Atypical teratoid rhabdoid tumor of the central nervous system (CNS
ATRT) is a malignancy that commonly affects young children. The biological mechanisms contributing
to tumor aggressiveness and resistance to conventional therapies in ATRT are unknown. Previous
studies have shown the activity of insulin like growth factor-I receptor (IGF-1R) in ATRT tumor
specimens and cell lines. IGF-1R has been shown to cross-talk with other receptor tyrosine
kinases (RTKs) in a number of cancer types, leading to enhanced cell proliferation.

Objective:
This study aims to evaluate the role of IGF-1 receptor cross-talk in ATRT biology and the
potential for therapeutic targeting.

Methods:
Cell lines derived from CNS ATRT specimens were analyzed for IGF-1 mediated cell
proliferation. A comprehensive receptor tyrosine kinase (RTK) screen was conducted following
IGF-1 stimulation. Bioinformatic analysis of publicly available cancer growth inhibition data to
identify correlation between IC50 of a VEGFR inhibitor and IGF-1R expression.

Results:
Comprehensive RTK screen identified VEGFR-2 cross-activation following IGF-1 stimulation.
Bioinformatics analysis demonstrated a positive correlation between IC50 values of VEGFR
inhibitor Axitinib and IGF-1R expression, supporting the critical influence of IGF-1R in modulating
response to anti-angiogenic therapies.

Conclusion:
Overall, our data present a novel experimental framework to evaluate and utilize receptor
cross-talk mechanisms to select effective drugs and combinations for future therapeutic trials in
ATRT.
Herbal medicine: current progress, and challenges
Authors: Barkat Abul , Goyal Anjali , Harshita Abul , Pottoo Faheem Hyder , Beg Sarwar , Ahmad Farhan J 
Published: November 2019

:
Phytomedicine or herbal medicines have played an important role in disease treatment because of high therapeutic potential and better patient compliance. Although the practice of phytomedicne greatly affected during the dawn of the scientific era, there is a revival of interest in its potential by late 20th century, especially in the search of new drugs. Herbal drugs and its derivatives represent more than 50% of entire drugs in modern healthcare system. Because of the low success rate and huge capital investment hinders the further application of phytomedicine in disease treatment. In recent time, an increase of interest in the herbal meidicine has been observed by pharmaceutical industry to search novel phytotherapeutics. In the search of new phytomedicine there are several challenges identified like-the reproducibility of pharmacological activity of herbal extracts, toxicity and adverse effects, adulteration and contamination, herb–drug interactions issues; poor scientific and regulatory standards. In this review, we illustrated a short overview on herbal medicine history, current clinical perspective, pharmaceutical and regulatory challenges as well as its clinical presence. Moreover, problems encountered in drug discovery from herbal resources and its possible solutions are described.
An LC-MS/MS Method for Determination of Triple Drugs Combination of Valsartan, Amlodipine and Hydrochlorothiazide in Human Plasma for Bioequivalence Study
Authors: Said Rana , Arafat Basel , Arafat Tawfiq , Mallah Eyad 
Published: November 2019

Background:
Current guidelines for the treatment of hypertension recommend combination
therapy, which intends to control blood pressure and enhance cardiovascular protection.

Materials and Methods:
A sensitive, reliable and selective tandem mass spectrometry (LC-MS/MS)
method has been developed for simultaneous quantification of amlodipine (AML), valsartan (VAL)
and hydrochlorothiazide (HCTZ) in human plasma. The chromatographic system was equipped with
ACE 5 C8 (50 X 2.1 mm) column and utilized a mobile phase composition of 0.5 mM Ammonium
Chloride & 0.04% FA-Methanol (45:55% v/v). The method used three internal standards; AML-D4,
HCTZ-D2 C13 and VAL-D3 with 10% intra- and inter-day precision, and 6% bias for all the analytes.

Results:
The assay was found to be linear with R-2 > 0.998, and the limits of quantification for AML,
VAL and HCTZ were 0.2, 50.0 and 2.0 ng/mL, respectively. The analytes were found to be stable in
plasma samples over short and long term storage.

:
The developed method is rapid with a run time of 3.5 min and cost-effective since the simple sample
preparation method is adopted. This method was successfully applied for the bioequivalence study of
AML, VAL, and HCTZ in human plasma after administration of the fixed-dose combination tablet of
(10/160/25 mg). Pharmacokinetic parameters (Cmax and AUC0-72) for AML and (Cmax, AUC0-t, AUC0-∞)
for VAL and HCTZ were used for bioequivalence assessment. These were determined by noncompartmental
analysis of concentration data.

Conclusion:
The result showed 90% confidence intervals (obtained by ANOVA) which were within
the predefined ranges. As a consequence, this method can be successfully applied for measuring and
quantifying a large number of samples.