bluuebunny/crossref_metadata_2025_split huggingface dataset:
Long non-coding RNA PTCSC3 inhibits human oral cancer cell proliferation by inducing apoptosis and autophagy
Authors: Zhang Hongmei , Wang Jiang , Xun Wenxing , Wang Jia , Song Wei , Wang Xiaoxia 
Published: June 2020

IntroductionLong non-coding RNAs (lncRNAs) have gained increased attention due to the discovery of their roles in cancer-related processes. LncRNA PTCSC3 has been shown to have tumour-suppressive effects in thyroid cancer and glioblastoma. This study investigated the role of lncRNA PTSC3 in human oral cancer.Material and methodsCell viability was determined by MTT assay. The induction of apoptosis was confirmed by 4,6-diamidino-2-phenylindole (DAPI) and Annexin V/PI assays. Ultrastructural analysis was performed by electron microscopy. Transwell assay was used to monitor the invasion of oral cancer cells.ResultsThe results revealed significant (p < 0.05) suppression of PTCSC3 expression in human oral cancer tissues and cell lines. The overexpression of PTCSC3 caused a significant (p < 0.05) decline in the proliferation of the human oral cancer cells via induction of apoptotic cell death which was accompanied by remarkable enhancement of Bax and suppression of Bcl-2. The electron microscopic analysis showed the development of autophagic vesicles in both the SCC-1 and SCC-9 cells indicative of autophagy. The western blotting analysis showed that PTCSC3 overexpression caused a remarkable increase in LC3B-I and Beclin 1 expression. PTCSC3 overexpression caused a significant (p < 0.05) decrease in invasion of the human SCC-1 and SCC-9 oral cancer cells. The invasion of the SCC-1 and SCC-9 cells was inhibited by 62% and 69% respectively.ConclusionsOverall, the evidence suggests that lncRNA PTCSC3 acts as a tumour suppressor in human oral cancer and suppresses oral cancer proliferation via induction of apoptosis and autophagy.
Genetic diversity and genetic origin of Lanping black-boned sheep investigated by genome-wide single-nucleotide polymorphisms (SNPs)
Authors: Xiong Heli , He Xiaoming , Li Jing , Liu Xingneng , Peng Chaochao , Xi Dongmei , Deng Weidong 
Published: June 2020

Abstract. Lanping black-boned sheep was first discovered in the 1950s in Lanping county of China and characterized by black pigmentation on skin and internal organs. Due to the novel and unique trait, the genetic background of Lanping black-boned sheep is of great interest. Here, we genotyped genome-wide SNPs (single nucleotide polymorphisms) of Lanping black-boned sheep and Lanping normal sheep using Illumina OvineSNP50 BeadChip to investigate the genetic diversity and genetic origin of Lanping black-boned sheep. We also downloaded a subset SNP dataset of two Tibet-lineage sheep breeds and four other sheep breeds from the International Sheep Genomics Consortium (ISGC) as a reference for interpreting. Lanping black-boned sheep had a lower genetic diversity level when compared to seven other sheep breeds. Principal component analysis (PCA) showed that Lanping black-boned sheep and Lanping normal sheep were clustered into the Asian group, but there was no clear separation between the two breeds. Structure analysis demonstrated a high ancestry coefficient in Lanping black-boned sheep and Lanping normal sheep. However, the two populations were separated into two distinct branches in a neighbor-joining (NJ) tree. We further evaluated the genetic divergence using population FST, which showed that the genetic differentiation that existed between Lanping black-boned sheep and Lanping normal sheep was higher than that between Tibet sheep and Changthangi sheep, which revealed that Lanping black-boned sheep is a different breed from Lanping normal sheep on the genetic level. In addition, structure analysis and NJ tree showed that Lanping black-boned sheep had a relatively close relation with Tibet sheep. The results reported herein are a first step toward understanding the genetic background of Lanping black-boned sheep, and it will provide informative knowledge on the unique genetic resource conservation and mechanism of novel breed formation.
H migration in peroxy radicals under atmospheric conditions
Authors: Vereecken Luc , Nozière Barbara 
Published: June 2020

Abstract. A large data set of rate coefficients for H migration in
peroxy radicals is presented and supplemented with literature data to
derive a structure–activity relationship (SAR) for the title reaction
class. The SAR supports aliphatic RO2 radicals; unsaturated
bonds and β-oxo substitutions both endocyclic and exocyclic to the
transition state ring; and α-oxo (aldehyde), –OH, –OOH, and
–ONO2 substitutions, including migration of O-based hydrogen atoms.
Also discussed are –C(=O)OH and –OR substitutions. The SAR allows
predictions of rate coefficients k(T) for a temperature range of 200 to 450 K,
with migrations spans ranging from 1,4 to 1,9-H shifts depending on the
functionalities. The performance of the SAR reflects the uncertainty of the
underlying data, reproducing the scarce experimental data on average to a
factor of 2 and the wide range of theoretical data to a factor of 10 to 100,
depending also on the quality of the data. The SAR evaluation discusses the
performance in multi-functionalized species. For aliphatic RO2, we also
present some experimental product identification that validates the expected
mechanisms. The proposed SAR is a valuable tool for mechanism development and
experimental design and guides future theoretical work, which should allow for
rapid improvements of the SAR in the future. Relative
multi-conformer transition state theory (rel-MC-TST) kinetic theory is introduced as an aid for
systematic kinetic studies.
Long-term brown carbon and smoke tracer observations in Bogotá, Colombia: association with medium-range transport of biomass burning plumes
Authors: Rincón-Riveros Juan Manuel , Rincón-Caro Maria Alejandra , Sullivan Amy P. , Mendez-Espinosa Juan Felipe , Belalcazar Luis Carlos , Quirama Aguilar Miguel , Morales Betancourt Ricardo 
Published: June 2020

Abstract. Light-absorbing aerosols emitted during open biomass burning (BB) events such as wildfires and agricultural burns have a strong impact on the Earth's radiation budget through both direct and indirect effects. Additionally, BB aerosols and gas-phase emissions can substantially reduce air quality at local, regional, and global scales, negatively affecting human health. South America is one of largest contributors to BB emissions globally. After Amazonia, the BB emissions from wildfires and agricultural burns in the grassland plains of northern South America (NSA) are the most significant in the region. However, few studies have analyzed the potential impact of NSA BB emissions on regional air quality. Recent evidence suggests that seasonal variations in air quality in several major cities in NSA could be associated with open biomass burning emissions, but it is still uncertain to what extent those sources impact air quality in the region. In this work, we report on 3 years of continuous equivalent black carbon (eBC) and brown carbon (BrC) observations at a hilltop site located upwind of the city of Bogotá, and we demonstrate its association with fires detected by the MODerate-resolution Imaging Spectroradiometer (MODIS) in a 3000 km × 2000 km domain. Offline PM2.5 filter samples collected during three field campaigns were analyzed to quantify water-soluble organic carbon (WSOC), organic and elemental carbon (OC∕EC), and biomass burning tracers such as levoglucosan, galactosan, and potassium. MODIS active fire data and HYSPLIT back trajectories were used to identify potential biomass burning plumes transported to the city. We analyzed the relationship between BrC, WSOC, water-soluble potassium, and levoglucosan to identify signals of the regional transport of BB aerosols. Our results confirm that regional biomass burning transport from wildfires occurs annually during the months of January and April. The seasonality of eBC closely followed that of PM2.5 at the city air quality stations; however, the observed seasonality of BrC is distinctly different to that of eBC and strongly associated with regional fire counts. The strong correlation between BrC and regional fire counts was observed at daily, weekly, and monthly timescales. WSOC at the measurement site was observed to increase linearly with levoglucosan during high BB periods and to remain constant at ∼2.5 µgC m−3 during the low BB seasons. Our findings show, for the first time in this region, that aged BB plumes can regularly reach densely populated areas in the Central Andes of northern South America. A source footprint analysis involving BrC observations, back trajectories, and remotely sensed fire activity shows that the eastern savannas in NSA are the main BB source region for the domain analyzed.
Effects of Strongly Enhanced Atmospheric Methane Concentrations in a Fully Coupled Chemistry-Climate Model
Authors: Stecher Laura , Winterstein Franziska , Dameris Martin , Jöckel Patrick , Ponater Michael , Kunze Markus 
Published: June 2020

Abstract. In a previous study the quasi-instantaneous chemical impacts (rapid adjustments) of strongly enhanced methane (CH4) mixing ratios have been analyzed. However, to quantify the influence of the respective slow climate feedbacks on the chemical composition it is necessary to include the radiation driven temperature feedback. Therefore, we perform sensitivity simulations	with doubled and fivefold present-day (year 2010) CH4 mixing ratios with the chemistry-climate model EMAC and include in a novel set-up a mixed layer ocean model to account for tropospheric warming. We find that the slow climate feedbacks counteract the reduction of the hydroxyl radical in the troposphere, which is caused by the strongly enhanced CH4 mixing ratios. Thereby also the resulting prolongation of the tropospheric CH4 lifetime is weakened compared to the quasi-instantaneous response considered previously. Changes in the stratospheric circulation evolve clearly with the warming of the troposphere. The Brewer-Dobson circulation strengthens, affecting the response of trace gases, such as ozone, water vapour and CH4 in the stratosphere, and also causing stratospheric temperature changes. In the middle and upper stratosphere, the increase of stratospheric water vapour is reduced with respect to the quasi-instantaneous response. Weaker increases of the hydroxyl radical cause the chemical depletion of CH4 to be less strongly enhanced and thus the in situ source of stratospheric water vapour as well. However, in the lower stratosphere water vapour increases more strongly when tropospheric warming is accounted for enlarging its overall radiative impact. The response of the stratospheric adjusted temperatures driven by slow climate feedbacks is dominated by these increases of stratospheric water vapour, as well as strongly decreased ozone mixing ratios above the tropical tropopause, which result from enhanced tropical upwelling. While rapid radiative adjustments from ozone and stratospheric water vapour make an essential contribution to the effective CH4 radiative forcing, the radiative impact of the respective slow feedbacks is rather moderate. In line with this, the climate sensitivity from CH4 changes in this chemistry-climate model setup is not significantly different from the climate sensitivity in carbon dioxide-driven simulations, provided that the CH4 effective radiative forcing includes the rapid adjustments from ozone and stratospheric water vapour changes.
Dissolved CH4 coupled to photosynthetic picoeukaryotes in oxic waters and to cumulative chlorophyll a in anoxic waters of reservoirs
Authors: León-Palmero Elizabeth , Contreras-Ruiz Alba , Sierra Ana , Morales-Baquero Rafael , Reche Isabel 
Published: June 2020

Abstract. Methane (CH4) emissions from reservoirs are
responsible for most of the atmospheric climatic forcing of these aquatic
ecosystems, comparable to emissions from paddies or biomass burning.
Primarily, CH4 is produced during the anaerobic mineralization of
organic carbon in anoxic sediments by methanogenic archaea. However, the
origin of the recurrent and ubiquitous CH4 supersaturation in oxic
waters (i.e., the methane paradox) is still controversial. Here, we
determined the dissolved CH4 concentration in the water column of
12 reservoirs during summer stratification and winter mixing to explore
CH4 sources in oxic waters. Reservoir sizes ranged from 1.18 to 26.13 km2. We found that dissolved CH4 in the water column varied
by up to 4 orders of magnitude (0.02–213.64 µmol L−1), and all oxic depths
were consistently supersaturated in both periods. Phytoplanktonic sources
appear to determine the concentration of CH4 in these reservoirs
primarily. In anoxic waters, the depth-cumulative chlorophyll a
concentration, a proxy for the phytoplanktonic biomass exported to
sediments, was correlated to CH4 concentration. In oxic waters, the
photosynthetic picoeukaryotes' abundance was significantly correlated to the
dissolved CH4 concentration during both the stratification and the
mixing. The mean depth of the reservoirs, as a surrogate of the vertical
CH4 transport from sediment to the oxic waters, also contributed
notably to the CH4 concentration in oxic waters. Our findings suggest
that photosynthetic picoeukaryotes can play a significant role in
determining CH4 concentration in oxic waters, although their role as
CH4 sources to explain the methane paradox has been poorly explored.
Hydrometeorological droughts in the Miño–Limia–Sil hydrographic demarcation (northwestern Iberian Peninsula): the role of atmospheric drivers
Authors: Sorí Rogert , Vázquez Marta , Stojanovic Milica , Nieto Raquel , Liberato Margarida L. R. , Gimeno Luis 
Published: June 2020

Abstract. Drought is one of the world's primary natural hazards because of its environmental, economic, and social impacts. Therefore, monitoring and prediction for small regions, countries, or whole continents are
challenging. In this work, the meteorological droughts affecting the
Miño–Limia–Sil hydrographic demarcation in the northwestern Iberian
Peninsula during the period of 1980–2017 were identified. For this purpose and to assess the combined effects of temperature and precipitation on
drought conditions, the 1-month standardized precipitation evapotranspiration index (SPEI1) was utilized. Some of the
most severe episodes occurred during June 2016–January 2017, September 2011–March 2012, and December 2014–August 2015. An empirical-orthogonal-function analysis revealed that the spatial variability of the SPEI1 shows strong homogeneity in the region, and the drought phenomenon consequently behaves in the same way. Particular emphasis was given to investigating atmospheric circulation as a driver of different drought conditions. To this aim, a daily weather type classification based on the Lamb weather type (LWT) classification was utilized for the entire Iberian Peninsula. Results showed that atmospheric circulation from the southwest, west, and northwest are directly related to wet conditions in the Miño–Limia–Sil hydrographic demarcation during the entire hydrological year. Contrastingly, weather types imposing atmospheric circulation from the northeast, east, and southeast are best associated with dry conditions. Anomalies of the integrated vertical flow of humidity and their divergence for the onset, peak, and termination of the 10 most severe drought episodes also confirmed these results. In this sense, the major atmospheric teleconnection patterns related to dry and wet conditions were the Arctic Oscillation, Scandinavian pattern, and North Atlantic Oscillation. Hydrological drought investigated through the standardized runoff index was closely related to dry and wet conditions revealed by the SPEI at shorter temporal scales (1–2 months), especially during the rainy months (December–April).
Whereof one cannot speak … but should try to
Authors: Hilb Claudia 
Published: June 2020

ABSTRACT
The multiplicity of voices reflecting on the actual crisis shows that we are not looking at extreme dichotomies but are rather facing the coexistence of contradictory elements, or elements in tension, or a state of paradox. The essay tries to list these contradictory elements—newness or repetition? solidarity or egoism? local or global? private or public? democratic or autocratic outcome? nature or culture?—in an attempt to organize the current perplexity.
COMMUNICATIVE CULTURE FORMATION OF FUTURE SOCIAL WORKERS IN THE EDUCATIONAL PROCESS OF PEDAGOGICAL UNIVERSITY
Authors: Omelchenko Svitlana , Chernukha Nadiia , Spivak Yaroslav , Spivak Liudmyla , Uteubaeva Elmira 
Published: June 2020

Article considers the essence of the process of communicative culture formation of future social workers in the educational process of the pedagogical university. The communicative culture is singled out and considered through an interdisciplinary approach in the professional education of social workers. The peculiarities of the communicative culture formation of future social workers in the educational process in the pedagogical university are highlighted. Emphasis is placed on the need to use various tools in the phenomenon under study, such as: verbal, non-verbal, information and communication. The students' attitude to the process of communicative culture formation of future social workers in the educational process of the pedagogical university are investigated. The results of the experimental study are described.
Preventie is niet vrijblijvend
Authors: Toebes B.C.A. 
Published: June 2020

Preventie is niet vrijblijvend. Over het preventie-oeuvre van Jos Dute
Doel van deze bijdrage is om bloot te leggen hoe Jos Dute heeft bijgedragen aan het preventiediscours. Na een bespreking van Dutes inaugurale rede volgt een uitgebreide analyse van infectieziektenregulering in het licht van de recente uitbraak van COVID-19. Dit gevolgd door een kortere analyse van de regulering van alcohol.
Over hulp bij zelfdoding
Authors: Gevers J.K.M. 
Published: June 2020

Over hulp bij zelfdoding. Enkele gedachten naar aanleiding van de recente uitspraak van het Bundesverfassungsgericht
Recent heeft het Duitse constitutionele hof een baanbrekende uitspraak gedaan waarin een wettelijke bepaling die het verlenen van hulp bij zelfdoding aan banden legt, met een beroep op het zelfbeschikkingsrecht nietig wordt verklaard. Volgens het hof zijn de mogelijkheden voor de wetgever op dit gebied grenzen te stellen zeer beperkt. Naar aanleiding van de uitspraak wordt ingegaan op de rol van de rechter, het oprukkend zelfbeschikkingsrecht en de positie van hulp bij zelfdoding ten opzichte van euthanasie.
Alternatieve behandeling en het toezicht van de IGJ
Authors: Hubben J.H. 
Published: June 2020

Alternatieve behandeling en het toezicht van de IGJ
In de Wkkgz (2006) is de IGJ aangewezen als toezichthouder op niet-BIG-registreerde alternatieve beroepsbeoefenaren. De wet kent een speciale, strenge toetsingsnorm voor de deze categorie behandelaars. Er valt moeilijk inzicht te krijgen in de wijze waarop de IGJ aan dit nieuwe toezicht gestalte geeft. De vraag rijst of de IGJ zich daarbij niet te terughoudend opstelt.
Ouderenzorg en recht op preventie
Authors: Roscam Abbing H.D.C. 
Published: June 2020

Ouderenzorg en recht op preventie
‘Voorzorg’ en samenwerking van het medisch en sociaal domein ten behoeve van ouderenzorg zijn essentieel. Gemeenten hebben een belangrijke taak in het formuleren van maatregelen bij gezondheidsbedreigingen. Risico’s als gevolg van bezuinigingen op thuiszorg kunnen worden voorkomen door de specialist ouderengeneeskunde in te schakelen. Preventief ouderenbeleid levert gezondheidswinst.
Abortion withdrawal of sexual violence pregnancy: the role of the sex offender
Authors: Pimentel Renata Macedo Martins , Drezett Jefferson , Macedo Jr Hugo , Andreoni Solange , Silva Rebeca Souza e 
Published: June 2020

Introduction: Unwanted pregnancy is a serious consequence for women who experience sexual violence. Although deciding on abortion is frequent in these cases, there is not much information on women who give up abortion in this circumstance.
Objective: To analyse the associated factors in abortion withdrawal of sexual violence pregnancy.
Methods: A cross-sectional epidemiological study with a convenience sample of adolescents and women with pregnancy due to sexual violence and requesting legal abortion between August 1994 and December 2012, at Hospital Pérola Byington, São Paulo, Brazil. Pregnant women who gave up abortion after receiving the procedure approval were included and, in another group, pregnant women who completed the abortion. The variables were selected from a digitized database and analyzed using SPSS 15.0 software. The outcome was abortion withdrawal. The study variables were age; low education level; gestational age; color/black ethnicity; not being united; declare religion; serious threat from the aggressor; known offender; and residence of the aggressor. Odds ratios with 95% confidence intervals were calculated. The analysis used Wald's chi-square test (?2W) and logistic regression with variable of interest defined as the known aggressor. The research was approved by the Research Ethics Committee of the Federal University of São Paulo, Opinion No. 6767.
Results: The study included 941 women, 849 (90.2%) who had an abortion and 92 (9.8%) who gave up after being approved. Age ranged from 10-46 years, mean 23.2 ± 7.9 years, gestational age 4-22 weeks, average 11.9 ± 4.5 weeks. Among those who gave up abortion, 12.0% were <14 years old; 50.0% had gestational age ? 13 weeks; 50.0% had low education; 14.2% were black; 90.2% single; 85.9% declared to have religion; 50.0% were threatened; 12.0% of the cases occurred at the perpetrator's residence and 53.3% of the victims were raped by known perpetrators. In logistic regression, the only significant variable was the known perpetrator, increasing the victim's chance of giving up abortion twice.
Conclusion: The known sex offender has influenced the woman or adolescent's decision to give up legal abortion.
Description of soft tissue artifacts and related consequences on hindlimb kinematics during canine gait
Authors: Lin Cheng-Chung , Wang Shi-Nuan , Lu Ming , Chao Tzu-Yi , Lu Tung-Wu , Wu Ching-Ho 
Published: June 2020

Background
Soft tissue artifacts (STAs) are a source of error in marker-based gait analysis in dogs. While some studies have revealed the existence of STAs in the canine hindlimb, STAs and their influence on kinematic gait analysis remain unclear.

Methods
Thirteen healthy Taiwan dogs affixed with twenty skin markers on the thigh and crus were recruited. Soft tissue artifacts and their influence on the determination of segment poses and stifle angles were assessed by simultaneously measuring marker trajectories and kinematics of the underlying bones via a model-based fluoroscopic analysis method.

Results
Markers on the thigh showed higher STAs than those on the crus, with root-mean-square amplitudes up to 15.5 mm. None of the tested marker clusters were able to accurately reproduce the skeletal poses, in which the maximum root-mean-square deviations ranged from 3.4° to 8.1°. The use of markers resulted in overestimated stifle flexion during 40–60% of the gait cycle and underestimated stifle flexion during 80–90% of the gait cycle.

Conclusions
Considerable magnitudes and effects of STAs on the marker-based 3D gait analysis of dogs were demonstrated. The results indicate that the development of error-compensation techniques based on knowledge regarding STAs is warranted for more accurate gait analysis.