bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transcriptomic and Metabolomic Analyses to Unravel Responses of Populus Simonii × P. Nigra to attack by Asian Gypsy moth Lymantria Dispar (Lepidoptera: Lymantriidae)
Authors: LV Yuntong , Sun Lili , Yin Jingjing , Jia Yanpeng , Du Hui , cao chuanwang 
Published: October 2020

Abstract
Background: Poplar is frequently attacked by herbivorous insects, including the Asian gypsy moth, Lymantria dispar. Here, we combined metabolomic and transcriptomic analysis to identify key genes and metabolites involved into the molecular mechanism of defensive enhancement against L. dispar herbivory on poplar. Results: The 3666 differentially expressed genes (DEGs, 1,799 up-regulated and 1,867 down-regulated) and 1,171 DEGs (695 up-regulated and 476 down-regulated) were specific in L. dispar herbivory and mechanical wounding, respectively. Moreover, the 9,108 and 7,656 ions were detected while 636 and 531 different ions were obtained using positive (pos) mode and negative (neg) mode, respectively. Among these ions, the 33 and 7 different ions were specific in L. dispar herbivory and mechanical wounding in pos mode while 46 and 4 different ions in L. dispar herbivory and mechanical wounding in neg mode, respectively. The 3,666 specific DEGs in L. dispar herbivory group were classified into phenylpropanoid and flavonoid secondary metabolism pathways by comprehensive networks between transcriptomes and metabolomes. Conclusions: The current findings greatly improve our understanding of the induced defensive response in poplar plants against L. dispar infestation, and will contribute to develop insect-resistance poplar varieties.
3D Printing Guide Plate for Accurate Hemicortical Bone Resection&nbsp;in Low-Grade Bone Sarcoma
Authors: Wu Hongwei , Li Xian'an , Yang Shuo , Bu Jie , Xu Xuezheng , Liu Jianfan , Wang Xin 
Published: October 2020

Abstract
Background: Surgical resection and reconstruction for low grade bone sarcoma in the metaphysis of the long bone remains challenging. We hypothesize that 3D printing osteotomy guide plate could assist to accurately resect the tumor lesion and save the joint function without higher recurrence rate. Methods: From January 2017 to August 2019, ten patients with low-grade malignant bone tumor in metaphysis of the limbs were treated with hemicortical resection using 3D printing guide plate and biological reconstruction. Results: Four patients were paracorticular osteosarcoma, five cases had highly differentiated chondrosarcoma, and one case was a chondromyxoid fibroma. Two of the cases involved the proximal humerus, while eight cases involved the distal femur. There was neither post-operation infection, internal fixation loosening, nor fracture occurrence in any of the patients. The Musculoskeletal Tumor Society score averaged at 27.1, while the International Society of Limb Salvage imaging score examination averaged 87.8%. Conclusions: Here, we demonstrate that the 3D printing osteotomy guide plate assisted hemicortical bone resection is a beneficial strategy to effectively resect the primary low-grade malignant bone tumors in the metaphysis of long bone and restore excellent joint function.
Self-evaluation of Health State in Chinese Navy of Different Fleets
Authors: Ding Yibo , Wu Shaoping , Wang Hao , Liu Jiluo , Liu Yan , Zhang Hongwei , Zhai Yujia , Tan Xiaojie , Cao Guangwen 
Published: October 2020

Abstract
Objective: To clarify physical and psychological health state and potential medical need of Chinese navy. Methods: A cross-sectional study was performed in the total of 438 personnel of two fleets in different latitudes in China by self-reported questionnaire. The physical and psychological states were investigated and the potential medical needs were explored.Results: The top was muscle and skeletal system disease, affecting to 59% of the population, followed by respiratory disease, skin disease, oral disease, et al. The frequency of oral disease increased gradually, with the first peak at 26-30 years old. Ophthalmic and Ear-nose-throat diseases increased rapidly over 40 years old. The frequencies of above diseases were higher in submarine of Fleet B than that of Fleet A (P<0.05). In Fleet A, the frequencies of influenza and diarrhea in submarine were higher than those in surface fleet (P<0.001). The corps with low psychological self-evaluation suffered more diseases than those with high evaluation (P=0.04). Conclusions: Fleets in different latitudes exhibited different disease frequencies. Submarine force was more susceptible to infectious disease than surface fleet possibly due to the closed environment. High effective medical care should be given according to the age threshold of 25 and 40.
Tanshinone IIA Combined with CsA Inhibit Myocardial Cell Apoptosis Induced by Renal Ischemia-Reperfusion Injury by Modulating Mitochondrial Function Through PI3K/Akt/Bad Pathway in Obese Rats
Authors: Tai He , Jiang Xiao-lin , Li Yue , Kong Liang , Yao Si-cheng , Song Nan , Lv Mei-jun , Wu Jin , Yang Ping , Yang Guan-lin , Kuang Jin-song , Lan Zhi-ming , Jia Lian-qun 
Published: October 2020

Abstract
Background: Acute myocardial injury (AMI), which is induced by renal ischemia-reperfusion (IR), is a significant cause of acute kidney injury (AKI)-related associated death. Obesity increases the severity and frequency of AMI and AKI. Tanshinone IIA (TIIA) combined with cyclosporine A (CsA) pretreatment was used to alleviate myocardial cell apoptosis induced by renal IR, and to determine whether TIIA combined with CsA would attenuate myocardial cell apoptosis by modulating mitochondrial function through the PI3K/Akt/Bad pathway in obese rats. Methods: Male rates were fed a high fat diet for 8 weeks to generate obesity. AKI was induced by 30 min of kidney ischemia followed 24 h of reperfusion. Obese rats were given TIIA (10 mg/kg·d) for 2 weeks and CsA (5 mg/kg) 30 min before renal IR. Related indicators were examined.Results: TIIA combined with CsA alleviated the pathohistological injury and apoptosis induced by renal IR in myocardial cells. In addition, TIIA combined with CsA improved cardiac function and decreased the serum myocardial enzyme spectrum in obese rats after renal IR. At the same time, TIIA combined with CsA improved mitochondrial function. Abnormal function of mitochondria was supported by decreases in the respiration controlling rate (RCR), intracellular adenosine triphosphate (ATP), oxygen consumption rate, and mitochondrial membrane potential (MMP), and increases in mitochondrial reactive oxygen species (ROS), opening of the mitochondrial permeability transition pore (mPTP), mitochondrial DNA damage, and mitochondrial respiratory chain complex enzymes (Ⅰ, Ⅱ, Ⅲ, Ⅳ, and Ⅴ). The injury of mitochondrial dynamic function was assessed by a decrease in Drp1, and increases in Mfn1 and Mfn2, and mitochondrial biogenesis injury was assessed by decreases in PGC-1, NRF1, and TFam. TIIA combined with CsA can attenuate apoptosis through modulating mitochondrial function through the PI3K/Akt/Bad pathway in obese rats.Conclusion: We used isolated mitochondria from rat myocardial tissues to demonstrate that myocardial mitochondrial dysfunction occurred along with renal IR to induce myocardial cell apoptosis; obesity aggravated apoptosis. TIIA combined with CsA attenuated myocardial cell apoptosis by modulating mitochondrial function through the PI3K/Akt/Bad pathway in obese rats.
Surface-based Map Plasticity of the Sensorimotor Cortex in Hip Disorder at Local and Extensive Levels: A Resting-state fMRI&nbsp;Study
Authors: Ma Jie , Hua Xu-Yun , Zheng Mou-Xiong , Wu Jia-Jia , Huo Bei-Bei , Xing Xiang-Xin , Feng Sheng-Yi , Li Bo , Xu Jian-Guang 
Published: October 2020

Abstract
Background: Pain is one of the manifestations of hip disorder and has been proven to lead to the remodeling of somatotopic map plasticity in the cortex. However, it’s not clear whether hip disorder with pain induces somatotopic map plasticity in the cortex. We aimed to evaluate the surface-based map plasticity of the somatotopic cortex in hip disorder at local and extensive levels by resting-state functional magnetic resonance imaging (rs-fMRI).Methods: 20 patients with osteonecrosis of the femoral head (ONFH) (12 males and 8 females, age= 56.80±13.60 years) with Visual Analogue Scale (VAS) scores ≥ 4 and 20 healthy controls (9 males and 11 females, age= 54.56±10.23 years) were enrolled in this study. rs-fMRI data and T1 imaging data were collected, and surface-based regional homogeneity (ReHo), seed-based functional connectivity (FC), cortical thickness and the volume of subcortical gray nuclei were calculated.Results: Compared with the healthy controls, the ONFH patients showed signiﬁcantly increased surface-based ReHo in areas distributed mainly in the left dorsolateral prefrontal cortex and frontal eye field, the right frontal eye field and the premotor cortex and decreased surface-based ReHo in the right primary motor cortex and primary sensory cortex. When the area with decreased surface-based ReHo in the frontal eye field and right premotor cortex was used as the regions of interest (ROI), compared with the controls, the ONFH patients displayed increased FC in the right middle frontal cortex and right inferior parietal cortex and decreased FC in the right precentral cortex and right middle occipital cortex. ONFH patients also showed signiﬁcantly decreased cortical thickness in the para-insular area, supplementary motor cortex area and frontal eye field and decreased volume of subcortical gray matter nuclei in the right nucleus accumbens (479.32±88.26 vs 539.44±68.36, P=0.026). Conclusions: Hip disorder patients showed cortical plasticity changes, mainly in sensorimotor and pain-related regions.
Yield Characteristics Strengthening and Yield Strength Anti-Dispersion Identification Based on Bending Deformation
Authors: Duan Yong-chuan , Qiao Hai-di , Zhang Fang-fang , Liu Yu , Tian Le , Yang Liu , Guan Ying-ping 
Published: October 2020

Abstract
The online derivation of material yield is important for consistency control of product quality. Compare with other deformation, bending deformation is more suitable for online identification. The transition portion of bending force stroke curve is related to the yield strength of material, but for a wide set of materials with different thickness and properties, its longer transition curve shows higher identification scattering. In this paper, the yield characteristics strengthening method and the new anti-dispersion identification method are studied to reduce the scattering. It is found that the yield characteristics are comprehensively affected by several factors. The higher yield strength, together with thinner thickness, can weaken the yield characteristics. The thicker material with wider die span can strengthen the yield characteristics. Window vector method (WV) and standardized fitting residual method (SFR) are proposed. Through the numerical study found that the yield load obtained by WV method shows a most accurate correlation with yield strength. A novel piecewise correlation equation is proposed and added a thickness variable to eliminate partial dispersion from varied thickness. Furthermore, this paper uses the experimental data to extract the generalization error of new correlation model fitted by yield loads from WV method, maximum error is below 12%. This method can improve the accuracy and efficiency of online ranking materials.
Silica-Based Nanoparticles Targeting Dendritic Cells for Cancer Immunotherapy
Authors: Liu Yajing , Yao Lintong , Zhang Yun , shen Wenhui , Chen Chunxia , Wang Hongfei , Gao Yanfeng , Qi Yuanming , Chen Zhenzhen 
Published: October 2020

Abstract
BackgroundVaccination is a promising anticancer strategy, but the limited delivery routes and short retention of antigens and immunomodulatory agents are problems that need to be solved in vaccine design. Because silicon nanoparticles have a tunable pore size and high loading capacity, they have been used in a variety of drug delivery systems, but their roles in tumor vaccine and tumor immunotherapy need to be examined.MethodsCD40 mAb was attached to mesoporous silica nanoparticles (MSNs) through covalent conjunction, and MSN-CD40/OVA/CpG nanoparticles were examined by Fourier transform-infrared spectroscopy, transmission electron microscopy and nanoparticle analyzer. In vitro functions of nanoparticles were detected by cytotoxicity, cellular uptake, DC maturation, cross-presentation and T cell priming. In vivo functions were monitored by tumor elimination, DC maturation, cross-presentation and T cell activity.ResultsWe encapsulated anti-CD40 monoclonal antibodies, ovalbumin (OVA) antigen, and a toll-like receptor-9 agonist (CpG) in mesoporous silica nanoparticles (MSNs). The resulting MSN-CD40/OVA/CpG nanoparticles were efficiently phagocytized by splenocytes and bone marrow-derived dendritic cells (BMDC). The MSN-CD40/OVA/CpG nanoparticles induced the BMDC to express the costimulatory molecules CD80 and CD86, and release tumor necrosis factor-α. We found that MSN-CD40/OVA/CpG nanoparticles correctly enhanced antigen cross-priming, and stimulated T cell proliferation and interferon γ (IFNγ) production in vitro. In vivo, the MSN-CD40/OVA/CpG nanoparticles strongly increased intracellular IFNγ secretion and its release from OVA257–264 peptide-specific splenocytes into the cell supernatant, induced dendritic cell expression of major histocompatibility complex-II, and stimulated lymphocyte CD80 and CD86 expression. The MSN-CD40/OVA/CpG nanoparticles also inhibited tumor growth, enhanced tumor infiltration of CD8+ and CD4+ T cells, and stimulated IFNγ secretion from splenocytes. In conclusion, we believe these MSN-CD40/OVA/CpG nanoparticles are a promising strategy for improving antigen cross-presentation, cytotoxic T lymphocyte immune activity, and anti-tumor immunotherapy.
Earliest Detection to Date of SARS-CoV-2 in Florida: Identification Together With Influenza Virus on the Main Entry Door of a University Building, February 2020
Authors: Lednicky John , Salemi Marco , Subramaniam Kuttichantran , Waltzek Thomas B , Sabo-Attwood Tara , Loeb Julia C. , Hentschel Shannon , Tagliamonte Massimo , Marini Simone , Alam Md. Mahbubul , Stephenson Caroline J. , Elbadry Maha , Morris Glenn 
Published: October 2020

Abstract
BackgroundQuestions persist about patterns of initial dissemination of SARS-CoV-2 in the United States in early 2020.MethodsIn February and March, 2020, environmental surface swab samples were collected from the handle of the main entry door of a major university building in Florida, as part of a pilot surveillance project screening for influenza. Samples were taken at the end of regular classroom hours, between the dates of February 1-5 and February 19-March 4, 2020. ResultsInfluenza H1N1pdm09 was isolated from the door handle on four of the 19 days sampled. Both SARS-CoV-2 and influenza virus were detected in the sample collected on February 21, 2020. Based on sequence analysis, the Florida SARS-CoV-2 strain (designated UF-11) was identical to strains being identified in Washington state during the same time period, while the earliest similar sequences were sampled in China/Hubei between Dec 30th 2019 and Jan 5th 2020. The first human case of COVID-19 was not officially reported in Florida until March 1st. In an analysis of sequences from COVID-19 patients in this region of Florida, there was only limited evidence of subsequent dissemination of the UF-11 strain. Identical or highly similar strains, possibly related through a common transmission chain, were detected with increasing frequency in Washington state between end of February and beginning of March. ConclusionsOur data provide further documentation of the rapid early spread of SARS-CoV-2, and underscore the likelihood that closely related strains were cryptically circulating in multiple U.S. communities before the first “official” cases were recognized.
Evaluation of a Participatory Research Informed Physical Activity Intervention Among Women from Disadvantaged Neighborhoods - A Pilot Study
Authors: Ramji Rathi , Rämgård Margareta , Carlsson Elisabeth , Shleev Sergey , Awad Eman , Criovic Stefan , Kottorp Anders 
Published: October 2020

Abstract
Background: Citizens living in disadvantaged neighborhoods are susceptible to chronic diseases associated with factors such as physical inactivity and poor nutrition. Women are considered more vulnerable in these situations owing to lack of access to health promotional activities catering their needs. This study aimed at evaluating the effects of a participatory research informed physical activity intervention on the health and related behavior of women living in disadvantaged neighborhoods in Southern Sweden. Methods: Forty women were invited to participate in a participatory research informed physical activity intervention delivered by health promoters and health ambassadors over a 3-month period. Quality of life was assessed using the WHOQOL-BREF questionnaire and questions regarding behavioral factors including frequency of leisure time physical activity, everyday activities, intake of fruit, vegetable, fish and soda were assessed at three time points including t1, prior to intervention start, t2 immediately post-intervention and t3, 6-month post-intervention. Furthermore, biomedical outcomes such as hemoglobin, blood pressure, resting heart rate, blood flow rate, body mass index, fat mass index and muscle mass index were also assessed at the same three time points. Wilcoxon’s Sign Rank Test was used to compare pre- and post-test scores. Results: There was an increase in the scores of the different domains of health related quality of life at t2 immediately post-intervention (p < .05), however the scores reversed at point t3. The behavioral factors including physical activity, everyday activities, and fruit and vegetable intake significantly improved at both follow-up time points post intervention compared to that ahead of the intervention (p < .05). Systolic and diastolic pressure, significantly decreased at 6-month follow-up (t3) compared to that at pre- intervention start (p < .05). There were no significant changes in other biomedical outcomes or behavioral factors. Conclusion: The participatory research informed physical activity intervention pilot tested among women in disadvantaged neighbourhoods promoted prolonged engagement in physical activity, improved diet and had a positive effect on physical health. However, perceived health seemed to depend on the social interactions among participants during organized group activities indicating that future activities should be of longer duration for achieving sustainable improvements in perceived health.
Pharmacological activation of GPR55 improved cognitive impairment, neuroinflammation, oxidative stress and apoptosis induced by lipopolysaccharide in mice
Authors: Wang Xin , Xiang Xiao Tong , Hu Jie , Wu Yu Mei , Li YueYue , Jin Shi Yu , Wu Xian 
Published: October 2020

Abstract
BackgroundNeuroinflammation, oxidative stress and apoptosis are implicated in the pathogenesis of Alzheimer’s disease (AD). The purpose of the present study was to investigate the neuroprotective effects and possible mechanism of G-protein coupled receptor 55 (GPR55) agonist, O-1602, on lipopolysaccharide (LPS)-induced cognitive deficits in mice. MethodsICR mice were treated with intracerebroventricular (i.c.v.) injection of LPS. Cognitive tests were performed, including the open field, Morris water maze, novel object recognition, and passive avoidance tests. The expression of GPR55, NF-κB p65, caspase-3, Bax and Bcl-2 were examined in the hippocampus by western blotting. Pro-inflammatory cytokines and microglia were detected by ELISA kit and immunohistochemical analyses, respectively. The malondialdehyde (MDA) level, and superoxide dismutase (SOD) activity were examined by assay kits. Furthermore, TUNEL-staining was used to detect neuronal apoptosis.ResultsI.c.v. injection of LPS exhibited impaired performance in the behavior tests, which were ameliorated by O-1602 treatment(2.0 or 4.0 μg/mouse, i.c.v.). Importantly, O-1602 reversed GPR55 down-regulation, decreased the expression of NF-κB p65, and suppressed the accumulation of pro-inflammatory cytokines and microglia activation, decreased malondialdehyde (MDA) level, and increased superoxide dismutase (SOD) activity. In addition, O-1602 also significantly decreased Bax and increased Bcl-2 expression as well as decreased caspase-3 activity and TUNEL-positive cells, suppressed neuronal apoptosis in the hippocampus of LPS-treated mice.Conclusionswe conclude that O-1602 may ameliorate LPS-induced cognition deficits via inhibiting neuroinflammation, oxidative stress and apoptosis mediated by NF-κB signaling in mice.
Knowledge Translation of Scholarly Publishing Impacts on Public Health
Authors: Ran Na 
Published: October 2020

Abstract
BACKGROUND: Although scholarly publishing plays a key role in learning, the role of knowledge translation of scholarly publishing with education and income on public health has not been well established. The objective was to describe how knowledge translation of scholarly publishing impacts on public health. METHODS: The correlations between the input data and the target data were firstly calculated. After the input data that is not correlated to the target have been removed, the principal component analysis will be performed to avoid multicollinearity problems in the input data. Finally, the multivariate regression method is used to fit the relationship between the principal components and the target data. Thus both dimensionality reduction and personalized optimization oriented a target can be done.RESULTS: After the public health in China is measured by Life expectancy and Death rate, the Pearson correlation coefficient, principal component analysis, and linear regression method have been performed. It proved that some activities of knowledge translation of scholarly publishing with a focus on health and well-being have the highest correlations with the first principal component. Results are also presented on that the first and the second principal component explain 99.3% of the variation (p<0.01) in Life expectancy and 92.8 % of the variation (p<0.01) in Death rate, respectively. CONCLUSIONS: Scholarly publishing, education, income, health expenditure, nurses, and midwives appear to have a similarly important effect on public health.
New Familial Cases of Karyomegalic Interstitial Nephritis With Mutations in the FAN1&nbsp;Gene
Authors: REJEB Imen , Jerbi Mouna , Jilani Houweyda , Gaied Hanène , Elaribi Yasmina , Hizem Syrine , Aoudia Raja , Hedri Hafedh , Zaied Chiraz , Abid Salwa , Bacha Hassen , BenAbdallah Taieb , BenJemaa Lamia , Goucha Rim 
Published: October 2020

Abstract
Karyomegalic interstitial nephritis (KIN) is a rare disease entity that was first described by Burry in 1974 and given this term by Mihatsch 1979 and al five years later. KIN is characterized by chronic tubulo-interstitial nephritis associated with enlarged tubular epithelial cell nuclei, which leads to progressive decline of renal function. The prevalence of this disease is less than 1% and its pathogenesis is unclear. KIN results from the mutation in the FAN 1 (FANCD2/FANCI-Associated Nuclease 1) gene, a gene involved in the DNA damage response pathway, particularly in the kidney. KIN seems to be rather multifactorial involving an environmental factor Ochratoxin A (OTA) but especially a genetic predisposition. In this article, we report 6 additional cases of KIN with histological evidence in 3 patients. Family clustering and differences in susceptibility to develop the nephropathy in spite of a high OTA contamination in all subjects show that there is a genetic susceptibility. So we completed our explorations by a genetic study. This study allowed us to identify novel mutations in the FAN1 gene in the affected members. To our best knowledge, this is the first Tunisian study involving familial cases of KIN with mutations on the FAN1 gene.