bluuebunny/crossref_metadata_2025_split huggingface dataset:
25 Cooking method and muscle type impact volatile compound development in beef steaks
Authors: Vierck Kelly R , Legako Jerrad F , Brooks J Chance 
Published: November 2020

Abstract
The objective of this study was to determine the influence of dry heat cookery on beef flavor development of multiple beef muscles. Beef strip loins, top sirloin butts, tenderloins, shoulder clods, and chuck rolls were collected from USDA Low Choice carcasses (Small00-Small100 marbling; n = 20). Subprimals were wet aged in the dark for 21 d at 0 - 4℃. Following aging, subprimals were fabricated into 2.54 cm thick steaks of the following muscles: Gluteus medius (GM), Infraspinatus (IF), Longissimus lumborum (LL\), Psoas major (PM), Serratus ventralis (SV), and Triceps brachii (TB). Steaks were cooked to a medium degree of doneness (71℃) on one of four randomly assigned cooking methods: charbroiler grill (CHAR), clamshell grill (CLAM), convection oven (OVEN), or salamander broiler (SALA). Volatile compound analysis was conducted using gas chromatography-mass spectrometry with solid phase microextraction. Data were analyzed as a split plot arrangement, with subprimal serving as the whole plot factor and cooking method serving as the subplot factor. The majority of compounds evaluated were impacted by the cooking method main effect (n = 21), followed by the cooking method × muscle interaction (n = 18), and muscle (n = 12). Charbroiler GM steaks produced the greatest amount of methional, 2,5-dimethylpyrazine, 3-ethyl-2,5-dimethylpyrazine, and 2-ethyl-3,5-dimethylpyrazine compared to all other treatments (P &lt; 0.05). Charbroiler and CLAM steaks produced the greatest concentration of methylpyrazine and trimethylpyrazine, as well as Strecker aldehydes (P &lt; 0.05), indicating that more direct applications of heat increased Maillard product production. Steaks cooked using OVEN and SALA produced more lipid oxidation products, such as alkanes, aldehydes, and lipid derived ketones. Gluteus medius and SV steaks produced the greatest concentration of volatile compounds, with SV steaks producing an increased concentration of lipid derived compounds (P &lt; 0.05) and GM steaks producing a greater concentration of Maillard products (P &lt; 0.05). These data indicate that cooking method has a direct impact on the flavor profile produced by steaks.
176 The Suitability of Autoclave and Pressure Cooker Washing Methods in Comparison to ANKOM200 to Determine Neutral Detergent Fiber Using Different Feedstuffs and Washing Solutions
Authors: Adams Jordan , Norris Aaron B , Rivera Madeline E , Batista Luiz Fernando Dias , Tedeschi Luis O 
Published: November 2020

Abstract
The use of the in vitro gas production (IVGP) technique requires accurate determination of neutral detergent fiber (NDF) residue. However, the NDF determination using standard procedures are not always feasible for IVGP; thus requiring micro-NDF methods, which need autoclave (AC) and pressure cooker (PC) to boil the solution. A complete randomized design using a 3×3 factorial arrangement was implemented to investigate the effect of washing methods (WM: AC, PC, or ANKOM200) and solution ratios (WS: 100 mL neutral detergent solution (ND), 150 mL ND, or 100 mL H2O/g sample) to determine NDF residues, assuming ANKOM200 and 100 mL ND/g as the standard methodology. Each factor combination was performed in triplicate with a replicate being comprised of 12 bottles or bags (two blanks and five feedstuffs in duplicate). Feedstuffs were: alfalfa hay (AH), bermudagrass hay (BH), two high-forage rations (G1 and G2), and a high-concentrate ration (FR). Following each run, bottles were filtered to obtain the NDF. Data were analyzed by diet using a random coefficients model. An interaction of WM ′ WS was present for AH and G1 (P &lt; 0.01), G2 and FR had tendencies (P = 0.08 and 0.06, respectively), whereas BH demonstrated no interaction (P = 0.37). The PC with 100 mL or 150 mL did not differ from the standard methodology for AH, G1, G2, and FR. The BH demonstrated differences between WM and WS (P &lt; 0.01). The PC had lower NDF residue compared to the AC and ANKOM200, whereas H2O had substantially greater NDF residue relative to both ND ratios. We concluded that H2O is not a suitable substitute for ND solution regardless of the feedstuff. Both micro-NDF washing methods may be satisfactory depending on the type of feedstuff used, but further investigation is required.
11 Carcass Attributes and Performance of Poultry (Gallus gallus domesticus) fed Olive Pomace
Authors: Conn Benny J , Snokhous Payton , Carter Ethan , Urso Phil , Legako Jerrad F , Kelley Stanley F , Beverly Marcy M , Anderson Mark 
Published: November 2020

Abstract
In livestock production systems, feed is the most expensive production input. The goal of any producer is to find feed that provides a balance of nutrients to meet requirements for maintenance, growth, and performance while efficiently contributing favorable carcass attributes at a low cost. Olive pomace, a by-product of the olive industry, can serve as a source of fat (14%), protein (3.6%), and fiber (44.5%). The objective of this study was to determine adipose tissue content and sensory attributes of chicken breast (Phase I), as well as determine growth of growing chickens fed a mixture of commercial feed and olive pomace (Phase II). In Phase I, mature broilers were fed rations consisting of 25%, 50%, and 75% olive pomace blended with standard poultry feed for 27d. After harvest, a fat sample was taken for fatty acid analysis, and a breast was taken from each bird for sensory analysis. The only difference in fatty acid composition was in the 50% treatment, which had lower amounts of docosahexaenoic acid and higher amounts of decanoic acid. No significant differences were detected between treatment groups in the sensory analysis (P = 0.79). The young chickens (n = 40) were divided into three groups and fed a standard diet (control), or a standard diet with either 25% or 75% replaced with olive pomace. They were fed ad libitum for 35d; total feed consumed was measured daily and bodyweight (BW) was recorded weekly. No difference was detected between control and 25% in BW, but the 75% was lighter (P &lt; 0.01) than the other groups. Additionally, 75% consumed more feed (0.91/lb/bird/d; P &lt; 0.01) than any other group with no difference between control (0.64/lb/bird/d) and 25% (0.71/lb/bird/d). The results show that olive pomace can be utilized in chicken rations and will not affect growth or the quality of the end product.
115 Nitrogen fertilizer effects on above ground sward characteristics and beef heifer performance from native warm-season grass blends
Authors: Chappell Caroline , Marks Landon , Mason Katie , Mullenix Mary K , Dillard Sandra L , Muntifering Russ B 
Published: November 2020

Abstract
A 2-yr study was conducted at Black Belt Research and Extension Center in Marion Junction, AL, to evaluate the effect of nitrogen (N) fertilizer application rate on forage production characteristics, nutritive value, and animal performance of beef heifers grazing a mixture of native warm-season grasses (NWSG) including big bluestem, little bluestem, and indiangrass. Six, two-hectare plots were randomly assigned to one of two treatments (0 or 67 kg N ha-1 applied in early April; n = 3 replications per treatment). Paddocks were continuously stocked with four weaned Angus × Simmental beef heifers (initial BW 288 ± 7 kg) from late May/early June through mid-to-late August during 2018 (73 grazing d) and 2019 (70 grazing d), respectively. Put-and-take cattle were used to manage forage to a target of 38 cm. Forage mass and canopy heights were collected every two weeks during the trial. Visual ground cover ratings, canopy light interception, and botanical composition were measured at the beginning and end of the trial in each year. Hand-plucked samples were collected every two weeks during the grazing trial to determine forage nutritional value. Data were analyzed using the PROC MIXED procedure in SAS 9.4, and differences were declared significant when P ≤ 0.05. Nitrogen fertilized NWSG had greater crude protein (P &lt; 0.0001), sward heights (P = 0.0003), and canopy light interception at the beginning of the season (P = 0.0049) compared to non-fertilized paddocks. However, there were no differences (P ≥ 0.05) among N-fertility treatments for mean forage mass, heifer ADG, or BCS across the 2-yr study. Botanical composition data indicated that indiangrass decreased from 64% to 61% (P = 0.0022) and weed pressure increased from 11% to 15% (P = 0.0064) across the summer grazing season. Canopy light interception decreased by 51% from early June to August in fertilized NWSG and 26% in unfertilized paddocks, respectively. These data illustrate that NWSG systems may provide a viable grazing system in the summer months under reduced N inputs.
87 Evaluation of two stocking rates for grazing heifers on mixed sward native warm-season grass pastures in the Black Belt Prairie region
Authors: Parish Jane A , Yankey Kalisha C , Durst Libby S 
Published: November 2020

Abstract
Optimal use of native warm-season grasses in pasture systems involves stocking grazing livestock at suitable rates. The study objective was to evaluate forage nutritive value and heifer ADG at two stocking rates on mixed-sward pastures of big bluestem (Andropogon gerardi Vitman), little bluestem (Andropogon scoparius), and indiangrass (Sorghastrum nutans L.). Pastures (3 replications) were stocked for 56 d during June and July in 2 yr with crossbred (Bos taurus) heifers (n = 24 heifers/year) stratified by initial BW (288.3 ± 1.7 kg) to one of two continuous stocking rates: 1.9 heifers/ha (HIGH) and 1.2 heifers/ha (LOW). Mean forage nutritive values on a DM basis were not different between HIGH and LOW stocking rates, respectively, for CP (7.0 ± 0.2% vs 6.7 ± 0.2%; P = 0.27), ADF (41.0 ± 0.6 vs. 41.4 ± 0.6; P = 0.64), NDF (69.9 ± 0.5 vs. 68.7 ± 0.5; P = 0.09), or relative feed value (RFV) (76.0 ± 1.0 vs. 76.9 ± 1.0; P = 0.53). There was a year effect (P &lt; 0.01) and stocking rate x day effect (P &lt; 0.01) for TDN. At LOW, TDN decreased from day 0 to day 28 (P = 0.02) and day 28 to day 56 (P = 0.02). At HIGH, TDN decreased (P &lt; 0.01) from day 0 to day 28 but remained steady until day 56 (P = 0.21). There was a stocking rate x day interaction (P &lt; 0.01) with ADG: LOW day 28 to 56 (1.20 ± 0.08 kg/day), HIGH day 0 to 28 (0.89 ± 0.08 kg/day), HIGH day 28 to 56 (0.44 ± 0.08 kg/day), and LOW day 0 to 28 (0.30 ± 0.08 kg/day). Further assessment of cattle ADG using more divergent stocking rates and plant persistence measures is warranted to inform ideal native grass stocking rate recommendations.
5 Animal performance in grass monoculture or silvopasture systems using tree legumes
Authors: da Silva Izabela Aline Gomes , Dubeux Jose Carlos Batista , de Mello Alexandre C Leão , da Cunha Márcio Vieira , dos Santos Mércia Ferreira , Apolinário Valéria Oliveira , da Silva Gleise Medeiros , de Freitas Erinaldo Viana , DiLorenzo Nicolas 
Published: November 2020

Abstract
Silvopasture systems (SPS) area management option to enhance delivery of ecosystem services and diversification of income. This study evaluated productive responses of signalgrass (Urochloadecumbens Stapf.) in monoculture or in SPS in the sub-humid tropical region of Brazil during six months of the rainy season. The experimental design was randomized complete block with three replications. Treatments were signalgrass + Mimosa caesalpiniifolia Benth. (SPS-Mimosa); signalgrass + Gliricidia sepium (Jacq.) (SPS-Gliricidia); and signalgrass monoculture. Cattle were managed under continuous stocking with variable stocking rate. Response variables included herbage mass (HM), herbage accumulation (HA), stocking rate (SR), average daily gain (ADG), and gain per area (GPA). Herbage mass (HM) was greater (P &lt; 0.0001) on signal grass monoculture (2045 kg DM/ha) than in SPS (1370 kg DM/ha, average for both SPS). Herbage accumulation rate was similar for signalgrass in monoculture and SPS-Gliricidia (avg. 61 kg DM ha-1 d-1), and both were greater than SPS-Mimosa (37 kg DM ha-1 d-1). Average daily gain was greater(P &lt; 0.0001) for SPS-Gliricidia (1.1 kg head-1 d-1), followed by signalgrass in monoculture (0.9 kg head-1 d-1), and SPS-Mimosa (0.3 kg head-1 d-1). Stocking rate ranged from 0.6 animal units (1AU = 450-kg animal) per ha in April to 2.2 AUha-1 in June. Stocking rate and animal performance per area were similar for signalgrass in monoculture and SPS-Gliricidia, and both greater than SPS-Mimosa. Gliricidia enhanced animal performance and provided ecosystem services. Mimosa, however, reduced animal performance likely due to the competition with signalgrass. Mimosa trees, however, are an important source of income (timber used for fence posts) and that might overcome the losses in animal performance.
58 Effects of Diploid and Tetraploid Annual Ryegrass Cultivars on Cattle Performance, Herbage Mass, and Nutritive Value
Authors: Montgomery Sarah M , Karisch Brandi , Parish Jane A , Morrison Jesse , Baldwin Brian 
Published: November 2020

Abstract
Annual ryegrass (Lolium multiflorum Lam.) can be divided into two cytotypes: diploid (2n = 2x) and tetraploid (2n = 4x). Polyploid versions of a given plant species result in increased seedling vigor, more robust growth and, potentially, greater yield. While most data suggest no difference in yield between diploid and tetraploid cultivars, there is still potential for weight gain advantages thanks to increased non-structural carbohydrates in tetraploid cells. The objectives of this study were to compare weight gain of cattle grazing either diploid or tetraploid cultivars of annual ryegrass. Two diploid annual ryegrass cultivars (Marshall and Tam 90) and two tetraploid cultivars (Jumbo and Nelson) were planted in 0.81-ha pastures, each replicated four times. Four steers (mean initial BW = 214 kg) were randomly assigned to each pasture for an 82-d grazing period. Body weights were recorded starting at day 0 then every 28 days throughout the season. Data were analyzed in the GLM procedure of SAS using a significance level of α = 0.05. There were significant differences between cytotypes with respect to seasonal yield with diploids (9547.90 kg/ha) yielding greater than tetraploids (7762.00 kg/ha). As expected, ADF and NDF fractions were significantly less for tetraploids (ADF; 31.39%, NDF; 31.39%) compared to diploids (ADF; 33.75%, NDF; 53.25%). Tetraploids also contained significantly greater crude protein (13.50%) than diploids (12.90%). Cytotype had no effect on final average daily gain (ADG) (P = 0.9427). However, final ADG was significantly impacted by cultivar (P = 0.0134). Final ADG for Tam 90 (1.10 kg/d) was greater than Marshall (0.92 kg/d). Cattle grazing Nelson (1.04 kg/d) and Jumbo (0.99 kg/d) did not differ. Results show that regardless of differences in seasonal yield and nutritive value between cytotypes, there were no advantages in ADG for cattle grazing either cytotype.
97 Impact of Regionalized Forage Quality and Quantity and Feed Grain Water Use on the Daily Texas Beef Cattle Water Footprint and Supply Chain Efficiency
Authors: Menendez Hector M , Tedeschi Luis O 
Published: November 2020

Abstract
Livestock water use sustainability is a growing concern in the beef cattle sector. The Water Footprint Assessment (WFA) method has been used to quantify the water footprint (WF) of beef products but does not suggest any specific management strategies to decrease the WF of beef cattle (WFB) within and across the beef supply chain. The WFB is primarily influenced by forage and grain production water uses (m3/t), which are directly linked to dry matter (kg/d) and water intake (L/d) and cattle growth (kg/d). Therefore, the objective of this study was to assess the alteration of forage quality and above-ground biomass production (t/ha) of annual ryegrass (Lolium multiflorum) and bermudagrass (Cynodon dactylon), in addition to published WF estimates for corn (Zea mays) and soybean (Glycine max) production (m3/t) on the daily Texas WFB. A dynamic Texas Beef Water Footprint Model (TXWFB) was developed to predict WFB, using the System Dynamic methodology and equations from the Ruminant Nutrition System (RNS) and Beef Nutrient Requirements (NASEM) models. Results indicated that forage and crop biomass production is a high-leverage solution to offset the daily Texas WFB (%∆ = -55 to 130). The alteration of forage TDN had less of an impact on the Texas WFB (%∆ = -39 to 17). An ANOVA with a Tukey Posthoc test indicated that all WFB scenarios were significantly different (P &lt; 0.05) except for the low versus base TDN under low water use conditions scenario. The variability in the use of green and blue waters for grains indicated that the final WFB, in the feedlot phase, may be lower than the WFB in the cow-calf or stocker stages under certain efficiency conditions. Identification of high and low-leverage solutions may help Texas cattle stakeholders implement systemic strategies that aid in the efforts for sustainable beef water use.
111 Metsulfuron and variable nitrogen application rates on infected tall fescue: effects on stocker performance, forage yield, nutritive value, ergovaline concentration and botanical composition
Authors: Meng Kevin R , Bailey Eric , Zeltwanger Josh , Allen Hannah , Adams Mikaela , Brake Derek 
Published: November 2020

Abstract
Chemical seed-head suppression of endophyte infected tall fescue (Lolium arundinaceum) improves stocker cattle performance but may decrease forage yield. Spring nitrogen application increases tall fescue growth with a concomitant increase in ergot alkaloids, produced by the symbiotic endophyte Epichloë coenophiala. We hypothesized that greater amounts of nitrogen applied to tall fescue would increase forage yield and offset losses in forage production from chemical suppression of seed-heads with metsulfuron without effect on alkaloid concentration. Ninety-six steers (270 ± 20 kg) were randomly assigned to one of sixteen paddocks (1.8 ha) on April 18 and continuously grazed for 57 d. Paddocks were blocked by previous use (n = 4) and randomly assigned to one of four treatments; no metsulfuron, no nitrogen (NEGCON), metsulfuron with 0 (MET0), 67 (MET67), or 134 (MET134) kg/ha of ammonium nitrate, applied March 11. Steers grazing MET0 paddocks were removed 17 d early due to insufficient forage availability. Steer weight, forage yield, forage nutritive value and ergot alkaloids in forage samples were measured monthly. Seed-head frequency and species composition were determined in June. Metsulfuron application reduced (P &lt; 0.01) tall fescue seed-heads by 80%. Metsulfuron decreased (P = 0.03) ergovaline but ergovaline increased (P &lt; 0.01) at each monthly sampling across treatments. Nitrogen had no impact on ergovaline concentration (P = 0.50). Forage yield tended to be least (P = 0.07) for MET0, intermediate for NEGCON and MET67, and tended to be greatest for MET134 (P = 0.08). Steer ADG was not affected by treatment (P &lt; 0.80). Metsulfuron decreased NDF (P=0.02) regardless of fertilization rate. Forage CP increased with fertilization (P &lt; 0.01) and no differences were detected between NEGCON and MET0 (P = 0.45). Species composition was not impacted (P &gt;0.07) by treatment. Metsulfuron decreased seed-head growth and ergovaline concentration in tall fescue. Additional nitrogen fertilizer ameliorated forage yield lost to metsulfuron application but did not impact steer gain.
31 Determination of Optimal Harvest Weight for Mangalica Pigs Using a Serial Harvest Approach to Measure Growth Performance and Carcass Characteristics
Authors: Reeves Maegan A , Charlton Courtney E , Wilkerson Shannon R , Rehm John G , Brandebourg Terry D 
Published: November 2020

Abstract
Mangalica pigs are a popular niche breed given their reputation for superior quality pork. However, growth and carcass parameters for this breed are poorly documented. Our objective was to better characterize optimal harvest weights for the Mangalica breed. To accomplish this, a growth trial was conducted whereby pigs (n=56) were randomly distributed across stratified harvest weights (50, 57, 68, 82, 93, 102, 127 kg) in a completely randomized design. Pigs were fed standard finisher rations with individual daily feed intakes and weekly body weights recorded for all animals. At 24h postmortem, carcasses were split and ribbed with marbling and loin eye area (LEA) measured at the 10th rib. Primal cuts were fabricated and individually weighed. Fat back was separated from the loin and weighed. As expected, live weight significantly increased across weight class (P &lt; 0.0001). ADG was similar across classes up to 82 kg live weight before steadily declining with increasing weight class (P &lt; 0.0025). Likewise, feed efficiency did not differ between classes until weights heavier than 82 kg (P &lt; 0.03). LEA significantly increased by class up to 82 kg and then plateaued as harvest weight increased further (P &lt; 0.003). Marbling score significantly increased with increasing weight class up to 102 kg where they then plateaued (p &lt; 0.04). Fat back dramatically increased across all weight classes (p &lt; 0.0001) despite negligible increases in LEA or marbling after 102 kg. Primal cut weights for the ham (P &lt; 0.0001), loin (P &lt; 0.0001), Boston butt (P &lt; 0.0001), shoulder (P &lt; 0.0001), and belly (P &lt; 0.0001) all significantly increased with increasing live weight. These data suggest an optimal harvest weight occurs between 82 to 102 kg while offering little objective justification for the current practice of harvesting Mangalica pigs at much heavier live weights.
8 Assessment of consistency in quantification of ribonucleic acid across multiple methods
Authors: DeCarlo Andrea N , Parrish Joseph , Bridges William , Pratt Scott 
Published: November 2020

Abstract
Quantification and assessment of quality of ribonucleic acid (RNA) is essential for assays determining gene expression including qRT-PCR, northern blotting, and RNA sequencing. Methods assessing RNA abundance and quality vary in detection range and specificity, in part due to their differences in how quantification is achieved. Agilent chip technology utilizes fluorescent labeling of nucleic acids coupled to electrophoresis to separate RNA based on size. Nanodrop utilizes spectrophotometry assesses quantity nucleic acids based on absorption of light and allows for an estimation of purity using the A260/280 (nucleic acid to protein) ratio. RiboGreen assays utilize a fluorescent intercalating dye that specifically targets single-stranded RNA. The objective of this study was to determine the most accurate method of RNA quantification between these three assays. Male pituitary samples (n = 220) were collected at a local abattoir, anterior lobe isolated, flash frozen in methanol dry-ice bath, and stored at -80°C until further processing. Total RNA was isolated from all samples and samples were subjected to spectrophotometry, Agilent nano chip assay, and Ribogreen assay. Data were analyzed for differences in standard deviation of total yield (mg/g) across all methods and each pair of methods using an O’Brien test for unequal variances. Standard deviation differed due to quantification method (P = 0.0002). Over 5 times greater variability in quantification was observed between Agilent chip technology compared to nanodrop (P = 0.0036), 2 times greater between nanodrop and Ribogreen (P &lt; 0.0001), and 10 times greater variation between Agilent and Ribogreen methods (P = 0.0029). The Ribogreen assay provides the smallest variation in quantification from sample to sample and is the most reliable method out of the three technologies for quantification of RNA; however, it does not address issues of RNA quality.