bluuebunny/crossref_metadata_2025_split huggingface dataset:
Relationship between Energy Dosage and Apoptotic Cell Death by Modulated Electro-Hyperthermia
Authors: Kao Patrick Hung-Ju , Chen Chia-Hung , Tsang Yuk-Wah , Lin Chen-Si , Chiang Hsin-Chien , Huang Cheng-Chung , Chi Mau-Shin , Yang Kai-Lin , Li Wen-Tyng , Kao Shang-Jyh , Minnaar Carrie Anne , Chi Kwan-Hwa , Wang Yu-Shan 
Published: June 2020

AbstractModulated electro-hyperthermia (mEHT) is a form of mild hyperthermia (HT) used for cancer treatment. The principle utility of HT is the ability not only to increase cell temperature, but also to increase blood flow and associated pO2 to the microenvironment. While investigational evidence has shown the unique ability of mEHT to elicit apoptosis in cancer cells, in vivo and in vitro, the same trait has not been observed with conventional HT. There is dissension as to what allows mEHT to elicit apoptosis despite heating to only mild temperatures, with the predominant opinion in favor of increased temperature at a cellular level as the driving force. For this study, we hypothesized that in addition to temperature, the amount of electrical energy delivered is a major factor in induction of apoptosis by mEHT. To evaluate the impact of electrical energy on apoptosis, we divided generally practiced mEHT treatment into 3 phases: Phase I (treatment start to 10 min. mark): escalation from 25 °C to 37 °C Phase II (10 min. mark to 15 min. mark): escalation from 37 °C to 42 °C Phase III (15 min. mark to 45 min. mark): maintenance at 42 °C Combinations of mEHT at 18 W power, mEHT at 7.5 W power, water bath, and incubator were applied to each of the three phases. Power output was recorded per second and calculated as average power per second. Total number of corresponding Joules emitted per each experiment was also recorded. The biological effect of apoptotic cell death was assayed by annexin-V assay. In group where mEHT was applied for all three phases, apoptosis rate was measured at 31.18 ± 1.47%. In group where mEHT was only applied in Phases II and III, apoptosis rate dropped to 20.2 ± 2.1%. Where mEHT was only applied in Phase III, apoptosis was 6.4 ± 1.7%. Interestingly, when mEHT was applied in Phases I and II, whether Phase III was conducted in either water bath at 42 °C or incubator at 37 °C, resulted in nearly identical apoptosis rates, 26 ± 4.4% and 25.9 ± 3.1%, respectively. These results showed that accumulation of mEHT at high-powered setting (18 W/sec) during temperature escalation (Phase I and Phase II), significantly increased apoptosis of tested cancer cells. The data also showed that whereas apoptosis rate was significantly increased during temperature escalation by higher power (18 W/sec), apoptosis was limited during temperature maintenance with lower power (7.5 W/sec). This presents that neither maintenance of 42 °C nor accumulation of Joules by mEHT has immediate correlating effect on apoptosis rate. These findings may offer a basis for direction of clinical application of mEHT treatment.
Hierarchical biota-level and taxonomic controls on the chemistry of fossil melanosomes revealed using synchrotron X-ray fluorescence
Authors: Rossi Valentina , Webb Samuel M. , McNamara Maria E. 
Published: June 2020

AbstractFossil melanosomes, micron-sized granules rich in melanin in vivo, provide key information for investigations of the original coloration, taxonomy and internal anatomy of fossil vertebrates. Such studies rely, in part, on analysis of the inorganic chemistry of preserved melanosomes and an understanding of melanosome chemical taphonomy. The extent to which the preserved chemistry of fossil melanosomes is biased by biotic and abiotic factors is, however, unknown. Here we report the discovery of hierarchical controls on the inorganic chemistry of melanosomes from fossil vertebrates from nine biotas. The chemical data are dominated by a strong biota-level signal, indicating that the primary taphonomic control is the diagenetic history of the host sediment. This extrinsic control is superimposed by a biological, tissue-level control; tissue-specific chemical variation is most likely to survive in fossils where the inorganic chemistry of preserved melanosomes is distinct from that of the host sediment. Comparative analysis of our data for fossil and modern amphibians reveals that most fossil specimens show tissue-specific melanosome chemistries that differ from those of extant analogues, strongly suggesting alteration of original melanosome chemistry. Collectively, these findings form a predictive tool for the identification of fossil deposits with well-preserved melanosomes amenable to studies of fossil colour and anatomy.
Real-time Colorimetric Quantitative Molecular Detection of Infectious Diseases on Smartphone-based Diagnostic Platform
Authors: Yin Kun , Pandian Vikram , Kadimisetty Karteek , Zhang Xin , Ruiz Carlos , Cooper Kumarasen , Liu Changchun 
Published: June 2020

AbstractRapid diagnostics of infectious diseases and accurate identification of their causative pathogens play a crucial role in disease prevention, monitoring, and treatment. Conventional molecular detection of infectious pathogens requires expensive equipment and well-trained personnel, thus limiting its use in centralized clinical laboratories. To address this challenge, a portable smartphone-based quantitative molecular detection platform, termed “smart connected pathogen tracer” (SCPT), has been developed for pathogen monitoring and disease surveillance. The platform takes advantage of synergistically enhanced colorimetric loop-mediated isothermal amplification (LAMP) assay and smartphone-based color analysis, enabling simple, rapid and reliable nucleic acid quantification without need for expensive fluorescence detection equipment. The SCPT platform has been successfully applied to quantitatively detect: i) HPV DNA in saliva and clinical vaginal swab samples, and ii) HIV RNA in plasma samples with comparable sensitivity to state-of-art machine. It has also been demonstrated for disease spatiotemporal mapping and pathogen tracking by wireless connection and web-based surveillance. Such simple, cost-affordable, portable molecular detection platform has great potential for on-site early disease detection, remote healthcare monitoring, and epidemic surveillance.
Topographic distinction in long-term value signals between presumed dopamine neurons and presumed striatal projection neurons in behaving monkeys
Authors: Enomoto Kazuki , Matsumoto Naoyuki , Inokawa Hitoshi , Kimura Minoru , Yamada Hiroshi 
Published: June 2020

AbstractNigrostriatal dopamine (DA) projections are anatomically organized along the dorsolateral-ventromedial axis, conveying long-term value signals to the striatum for shaping actions toward multiple future rewards. The present study examines whether the topographic organization of long-term value signals are observed upon activity of presumed DA neurons and presumed striatal projection neurons (phasically active neurons, PANs), as predicted based on anatomical literature. Our results indicate that DA neurons in the dorsolateral midbrain encode long-term value signals on a short timescale, while ventromedial midbrain DA neurons encode such signals on a relatively longer timescale. Activity of the PANs in the dorsal striatum is more heterogeneous for encoding long-term values, although significant differences in long-term value signals were observed between the caudate nucleus and putamen. These findings suggest that topographic DA signals for long-term values are not simply transferred to striatal neurons, possibly due to the contribution of other projections to the striatum.
1,25(OH)2VitD3 supplementation enhances suppression of grass pollen-induced allergic asthma by subcutaneous and sublingual immunotherapy in a mouse model
Authors: Hesse Laura , Petersen Arjen H. , Oude Elberink Joanne N. G. , van Oosterhout Antoon J. M. , Nawijn Martijn C. 
Published: June 2020

AbstractAllergen specific immunotherapy (AIT) can provide long-term alleviation of symptoms for allergic disease but is hampered by suboptimal efficiency. We and others have previously shown that 1,25(OH)2-VitaminD3 (VitD3) can improve therapeutic efficacy of AIT. However, it is unknown whether VitD3 supplementation has similar effects in sublingual and subcutaneous immunotherapy. Therefore, we aimed to test VitD3 supplementation in both grass pollen (GP) subcutaneous-IT (SCIT) and sublingual-IT (SLIT) in a mouse model for allergic airway inflammation. To this end, GP-sensitized BALB/c mice received GP-SCIT or GP-SLIT with or without 10 ng VitD3, followed by intranasal GP challenges and measurement of airway hyperresponsiveness (AHR) and inflammation. VitD3 supplementation of GP-SCIT resulted in enhanced induction of GP-specific (sp)-IgG2a and suppression of spIgE after challenge. In addition, eosinophil numbers were reduced and levels of IL10 and Amphiregulin were increased in lung tissue. In GP-SLIT, VitD3 supplementation resulted in enhanced sp-IgG2a levels in serum, enhanced suppression of eosinophils and increased IL10 levels in lung tissue, as well as suppression of AHR to methacholine. These data show that VitD3 increases efficacy of both SCIT and SLIT, by enhancing induction of blocking antibodies and suppression of airway inflammation, underscoring the relevance of proficient VitD3 levels for successful AIT.
A copper-dependent compound restores ampicillin sensitivity in multidrug-resistant Staphylococcus aureus
Authors: Crawford Cameron L. , Dalecki Alex G. , Perez Mildred D. , Schaaf Kaitlyn , Wolschendorf Frank , Kutsch Olaf 
Published: June 2020

AbstractMulti-drug resistant Staphylococcus aureus, including methicillin-resistant S. aureus (MRSA), has become a worldwide, major health care problem. While initially restricted to clinical settings, drug resistant S. aureus is now one of the key causative agents of community-acquired infections. We have previously demonstrated that copper dependent inhibitors (CDIs), a class of antibiotics that are only active in the presence of copper ions, are effective bactericidal agents against MRSA. A second-generation CDI, APT-6K, exerted bactericidal activity at nanomolar concentrations. At sub-bactericidal concentrations, it effectively synergized with ampicillin to reverse drug resistance in multiple MRSA strains. APT-6K had a favorable therapeutic index when tested on eukaryotic cells (TI: > 30) and, unlike some previously reported CDIs, did not affect mitochondrial activity. These results further establish inhibitors that are activated by the binding of transition metal ions as a promising class of antibiotics, and for the first time, describe their ability to reverse existing drug resistance against clinically relevant antibiotics.
A unique hybrid characteristic having both pro- and anti-inflammatory phenotype transformed by repetitive low-dose lipopolysaccharide in C8-B4 microglia
Authors: Mizobuchi Haruka , Yamamoto Kazushi , Tsutsui Shoko , Yamashita Masafumi , Nakata Yoko , Inagawa Hiroyuki , Kohchi Chie , Soma Gen-Ichiro 
Published: June 2020

AbstractAlthough lipopolysaccharide (LPS) is regarded as an inducer of inflammation, previous studies have suggested that repetitive low-dose LPS has neuroprotective effects via immunomodulation of microglia, resident macrophages of brain. However, microglia transformed by the stimulus of repetitive low-dose LPS (REPELL-microglia) are not well characterized, whereas microglia transformed by repetitive high-dose LPS are well studied as an endotoxin tolerance model in which the induction of pro-inflammatory molecules is suppressed. In this study, to characterize REPELL-microglia, the gene expression and phagocytic activity of REPELL-microglia were analyzed with the murine C8-B4 microglia cell line. The REPELL-microglia were characterized by a high expression of pro-inflammatory molecules (Nos2, Ccl1, IL-12B, and CD86), anti-inflammatory molecules (IL-10, Arg1, Il13ra2, and Mrc1), and neuroprotective molecules (Ntf5, Ccl7, and Gipr). In addition, the phagocytic activity of REPELL-microglia was promoted as high as that of microglia transformed by single low-dose LPS. These results suggest the potential of REPELL-microglia for inflammatory regulation, neuroprotection, and phagocytic clearance. Moreover, this study revealed that gene expression of REPELL-microglia was distinct from that of microglia transformed by repetitive high-dose LPS treatment, suggesting the diversity of microglia transformation by different doses of LPS.
Comparison of radiotherapy techniques with flattening filter and flattening filter-free in lung radiotherapy according to the treatment volume size
Authors: Arslan Alaettin , Sengul Burak 
Published: June 2020

AbstractIn external radiotherapy (RT), the use of flattening filter-free (FFF) radiation beams obtained by removing the flattening filter (FF) in standard linear accelerators is rapidly increasing, and the benefits of clinical use are the issue of research. Advanced treatment techniques have increased the interest in the operation of linear accelerators in FFF mode. The differences of the beams with non-uniform dose distribution created by removing FF compared to the beams with uniform dose distribution used as a standard were examined. These differences were compared in the treatment plans of lung patients who have different planning target volumes (PTV). Clinac IX linear accelerator units were used. Twenty patients with previously completed treatment were divided into two groups depending on the size of the target volume. All patients underwent two different intensity-modulated RT (IMRT) plans using FF and FFF beams. The Wilcoxon Signed-Rank test was used to compare two different techniques (Significance p < 0.05). There was no statistically significant difference between the two techniques when looking at the D2%(Gy), D98%(Gy), D50%(Gy), homogeneity (HI), and conformity index (CI) data for both groups. When the critical organ doses were evaluated, there was a statistically significant difference only in the V20(%) values of the lungs, but these differences were not very large. Monitor unit (MU) data were found to be lower in FF planning, and treatment time was lower in FFF planning. Except for shorter treatment times, and of the lungs V20(%) value, in standard fractionated RT of lung cancer, there was no significant difference between the use of FFF and FF techniques for large and small target volumes.
Uptake, efflux, and toxicity of inorganic and methyl mercury in the endothelial cells (EA.hy926)
Authors: Liu Songnian , Tsui Martin Tsz-Ki , Lee Elizabeth , Fowler Josh , Jia Zhenquan 
Published: June 2020

AbstractCardiovascular disease (CVD) is the major cause of morbidity, mortality, and health care costs in the United States, and possibly around the world. Among the various risk factors of CVD, environmental and dietary exposures to mercury (Hg), a highly toxic metal traditionally regarded as a neurotoxin, has been recently suggested as a potential contributor towards human atherosclerotic development. In this study, we investigated the toxicity, type of cell death, dose-dependent uptake, and efflux of inorganic HgII (as HgCl2) and methylmercury or MeHg (as CH3HgCl) in EA.hy926 endothelial cells, as these two forms of Hg are often reported to be present in human blood among the general populations (~20–30% as HgII and ~70–80% as MeHg). Our results showed that HgII is more toxic than MeHg to the endothelial cells, owing to the higher uptake into the cytoplasm and perhaps importantly lower efflux of HgII by the cells, thus the “net” accumulation by the endothelial cells is higher for HgII than MeHg when exposed to the same Hg levels in the media. Furthermore, both HgII and MeHg were found to induce apoptotic and necrotic cell death. This study has important implications for the contributions of these two common Hg species to the development of atherosclerosis, an important process leading to CVD.
Entanglement-enhanced testing of multiple quantum hypotheses
Authors: Zhuang Quntao , Pirandola Stefano 
Published: June 2020

AbstractQuantum hypothesis testing has been greatly advanced for the binary discrimination of two states, or two channels. In this setting, we already know that quantum entanglement can be used to enhance the discrimination of two bosonic channels. Here, we remove the restriction of binary hypotheses and show that entangled photons can remarkably boost the discrimination of multiple bosonic channels. More precisely, we formulate a general problem of channel-position finding where the goal is to determine the position of a target channel among many background channels. We prove that, using entangled photons at the input and a generalized form of conditional nulling receiver at the output, we may outperform any classical strategy. Our results can be applied to enhance a range of technological tasks, including the optical readout of sparse classical data, the spectroscopic analysis of a frequency spectrum, and the determination of the direction of a target at fixed range.
MiR-4269 suppresses the tumorigenesis and development of pancreatic cancer by targeting ZEB1/OTX1 pathway
Authors: Sui Xin , Sui Zhenghui 
Published: June 2020

Abstract
As one of the most prevalent malignant tumors, pancreatic cancer (PC) is a leading fatal cancer worldwide. Surging evidence has unraveled that miRNAs are involved in the occurrence and progression of multiple cancers, including PC. The tumor suppressor effects of miR-4269 have been certified in gastric carcinoma. However, the potential function of miR-4269 remains largely unclear, which drives us to identify the role of miR-4269 in PC development. In the present study, we determined the expression pattern of miR-4269 in PC cells and normal cells. Results of RT-qPCR analysis illuminated that miR-4269 expression level in PC cells was lower than that in normal cells. Functional assays demonstrated that up-regulation of miR-4269 obviously inhibited the proliferation, migration and invasion of PC cells. In order to elucidate the mechanism governing miR-4269 in PC, we carried out bioinformatics analysis and further experimental investigations. Our results validated that ZEB1 was a direct target of miR-4269. Additionally, ZEB1 activated the transcription of OXT1. More importantly, miR-4269 attenuated the expression level of OXT1 via targeting ZEB1. Ultimately, our findings confirmed that miR-4269 served as a cancer suppressor in PC through regulation of ZEB1/OTX1 pathway, which suggested that miR-4269 might represent a promising target for the clinical treatment of PC.
miR-135a inhibits malignant proliferation and diffusion of non-small cell lung cancer cells by down-regulating ROCK1 protein
Authors: Zhao Yanan , Sun Xiaosong , Zhu Kun , Cheng Min 
Published: June 2020

Abstract
Objective: To seek the clinical significance and regulatory mechanism of miR-135a and Rho-associated protein kinase 1 (ROCK1) in non-small cell lung cancer (NSCLC).
Methods: NSCLC cells were purchased, and miR-135a-mimics, miR-135a-inhibitor, miR-NC, si-ROCK1 and Sh-ROCK1 were transfected into NSCLC cells HCC827 and NCI-H524. qRT-PCR and Western blot were used to detect the expression of miR-135a, ROCK1, Bax, Caspase3, Bcl-2, N-cadherin, vimentin and E-cadherin. MTT, scratch test, Transwell and flow cytometry were used to analyze the cell proliferation, migration, invasion and apoptosis.
Results: miR-135a was low expressed in serum of NSCLC group, while ROCK1 was opposite. miR-135a low level or ROCK1 high level was associated with poor prognosis of NSCLC and lower 3-year OS. Over-expression of miR-135a and inhibition of ROCK1 expression could control malignant growth and diffusion of cells and expression of Bcl-2, N-cadherin and vimentin proteins, and promote apoptosis and expression of Bax, Caspase3 and E-cadherin proteins. After transfection of miR-135a-mimics+sh-ROCK1 to HCC827 and NCI-H524, the malignant proliferation and diffusion behavior of the cells were not different from those of the miR-NC group with no transfection sequence. The double luciferase report revealed that miR-135a has a targeting relationship with ROCK1.
Conclusion: miR-135a is abnormally down-regulated in NSCLC. As a serum indicator, miR-135a has the potential to diagnose NSCLC and predict prognosis. The up-regulated expression of miR-135a protein can down-regulate the ROCK1 protein, inhibit the malignant proliferation, migration, invasion, EMT and other diffusion behaviors of NSCLC cells, and increase the apoptosis ability of cells.
Low‐temperature‐grown gallium arsenide photoconductors with subpicosecond carrier lifetime and photoresponse reaching 25 mA/W under 1550 nm CW excitation
Authors: Tannoury C. , Billet M. , Coinon C. , Lampin J‐F. , Peytavit E. 
Published: June 2020

The authors show in this Letter that photoconductors based on GaAs grown at low temperatures can exhibit photoresponses as high as 25 mA/W under continuous‐wave 1550‐nm‐wavelength illumination. It is achieved by using an optical Fabry–Pérot cavity in order to improve the external quantum efficiency and by decreasing the post‐growth annealing temperature down‐to 450°C.
Quantitative MR-Bildgebung zur Charakterisierung der Skelettmuskulatur
Authors: Engelke Klaus , Chaudry Oliver , Nagel Armin 
Published: June 2020

ZusammenfassungDieser Beitrag gibt einen Überblick über Magnetresonanztomographie-basierte (MRT-basierte) Methoden zur Quantifizierung der Muskeldegeneration. Neben Muskelvolumen kann mit Dixon-Bildgebung insbesondere der prozentuale Fettgehalt bestimmt werden. Daneben gibt es Ansätze, frühe Anzeichen einer Degeneration über die Verteilung des Entzündungsgrades oder der Natriumkonzentration in der Muskulatur zu visualisieren und quantifizieren. Bis auf die Natrium-Bildgebung werden diese Methoden bei Muskelerkrankungen routinemäßig zur Diagnose und Verlaufskontrolle eingesetzt.Im Bereich der Osteologie und Gerontologie wird zwar die Bedeutung der Muskel-Knochen-Einheit unter anderem für Frakturprädiktion und Gebrechlichkeit im Alter immer wieder betont, Degeneration der Muskulatur wird aber im Wesentlichen über extrinsische Parameter wie Muskelkraft und -funktion erfasst. Häufig benutzte intrinsische Parameter wie DXA Lean Mass oder Muskelvolumen, bestimmt mit CT oder MRT, korrelieren nur mäßig mit extrinsischen Parametern. Eine genauere Charakterisierung von Muskelqualität sollte dieses Manko aber beseitigen. Mit CT und MRT stehen entsprechende Methoden zur Verfügung, die jetzt aber in Studien zur altersassoziierten Muskeldegeneration, in Interventionsstudien und in Studien zur Frakturrisikoprognostik auch eingesetzt werden müssen.
Umweltgerechtigkeit: Subjektive Hitzebelastung als Folge des
Klimawandels in konträren Stadtquartieren
Authors: Looks Peggy , Borchers Peggy , Reinfried Franziska , Oertel Holger , Kugler Joachim 
Published: June 2020

Zusammenfassung
Hintergrund Bislang liegen für die Stadt Dresden keine Daten
zur Wahrnehmung des Klimawandels und der damit verbundenen Zunahme von
Hitzeperioden, die insbesondere gezielte Handlungsableitungen für
vulnerable Gruppen ermöglichen vor.
Methode Um diese Lücke zu schließen wurde 2017 eine
repräsentative Befragung der Bevölkerung in Dresden
durchgeführt. Zwei ausgewählte Stadtquartiere, die sich
sowohl städtebaulich als auch sozialstrukturell voneinander
unterscheiden, wurden im Rahmen dieses Beitrages miteinander verglichen. Im
Besonderen ging es um die Frage, ob eine Verteilungs- bzw.
Verfahrensgerechtigkeit vorliegt, was die Auswirkungen der subjektiven
Hitzebelastung sowie beeinflussender Faktoren anbelangt.
Ergebnisse Die Ergebnisse zeigen, dass sich insbesondere
Bewohner/innen des Stadtgebietes mit überwiegend
Plattenbaugebäuden, wenig Grünflächen und einem
erhöhten Anteil an sozial-benachteiligten Personen stärker
von sommerlicher Hitze belastet fühlen und zudem weniger
Möglichkeiten haben, sich den hohen Temperaturen anzupassen.
Schlussfolgerung Aus den Ergebnissen lassen sich neben
Stadtentwicklungsmaßnahmen v. a. auch Maßnahmen zur
Prävention hitzebedingter Erkrankungen ableiten. Diese erfordern
bspw. eine verstärkte Beratung und Aufklärung der Menschen
direkt vor Ort.