bluuebunny/crossref_metadata_2025_split huggingface dataset:
Asthma and COVID-19 - A systematic review
Authors: Mendes Natália F. , Jara Carlos P. , Mansour Eli , Araújo Eliana P. , Velloso Licio 
Published: November 2020

Abstract
Background. Severe coronavirus disease-19 (COVID-19) presents with progressive dyspnea, which results from acute lung inflammatory edema leading to hypoxia. As with other infectious diseases that affect the respiratory tract, asthma has been cited as a potential risk factor for severe COVID-19. However, conflicting results have been published over the last few months and the putative association between these two diseases is still unproven. Methods. Here, we systematically reviewed all reports on COVID-19 published since its emergence in December 2019 to June 30, 2020, looking into the description of asthma as a premorbid condition, which could indicate its potential involvement in disease progression. Results. We found 372 articles describing the underlying diseases of 161,271 patients diagnosed with COVID-19. Asthma was reported as a premorbid condition in only 2,623 patients accounting for 1.6% of all patients. Conclusions. As the global prevalence of asthma is 4.4%, we conclude that either asthma is not a premorbid condition that contributes to the development of COVID-19 or clinicians and researchers are not accurately describing the premorbidities in COVID-19 patients.
Loss of MiR-192-5p Initiates a Hyperglycolysis and Stemness Positive Feedback in Hepatocellular Carcinoma.
Authors: Gu Yuanzhuo , Ji Fubo , Liu Niya , Zhao Yongzhi , Wei Xiyang , Hu Shiyuan , Jia Wei , Wang Xin Wei , Budhu Anuradha , Ji Juling , Zhao Bin , Roessler Stephanie , Zheng Xin , Ji Junfang 
Published: November 2020

Abstract
Background: Emerging studies revealed that cancer stem cells (CSCs) possessed peculiar metabolic properties, which however remained largely unknown in hepatocellular carcinoma (HCC). Genetic silencing of liver-abundant miR-192-5p was a key feature for multiple groups of CSC-positive HCCs. We thus aimed to investigate essential metabolic features of hepatic CSCs via using HCCs with miR-192-5p silencing as a model.Methods: Datasets from two independent HCC cohorts were used. Data integration analyses of miR-192-5p with metabolome and mRNA transcriptome data in HCC Cohort 1 were performed to investigate miR-192-5p related metabolic features, which was further validated in Cohort 2. Cellular and molecular assays were performed to examine whether and how miR-192-5p regulated the identified metabolic features. Co-culture systems consisting of HCC cells and LX2 (human hepatic stellate cell line) or THP1 (human monocyte cell line) were established to explore effects of the identified metabolic properties on stemness features of HCC cells via interacting with co-cultured non-tumor cells.Results: High levels of glycolysis-related metabolites and genes were present in HCCs with low miR-192-5p and CSC-positive HCCs in two independent HCC cohorts. miR-192-5p knockout cells displayed CSC features and miR-192-5p loss led to an enhanced glycolytic phenotype via upregulating three bona fide targets, GLUT1 and PFKFB3 (two glycolytic enzymes) and c-Myc (regulating glycolytic genes’ expression). Meanwhile, c-Myc suppressed miR-192-5p transcription, ensuring a low-miR-192-5p/high-c-Myc loop to maintain hyperglycolysis. Moreover, over-produced lactic acid from hyperglycolytic HCC cells stimulated the ERK phosphorylation of co-cultured LX2 and THP1 non-tumor cells partially via NDRG3 and MCT1, which in turn promoted cell malignancy and stemness of HCC cells. Consistently, HCC patients with low level of miR-192-5p in their tumor tissues and high level of NDRG3 or MCT1 in their non-tumor tissues had the shortest overall survival.Conclusions: In CSC-positive HCCs, miR-192-5p loss enhanced glycolysis and over produced lactate might further increase HCC malignant features via interacting with environmental non-tumor cells.
Comparison of surgical, functional and oncological outcomes of innovatively “three-port” and traditionally “four-port” laparoscopic radical prostatectomy in patients with prostate cancer
Authors: Xu Ben , Cheng Si-da , Peng Yi-ji , Zhang Qian 
Published: November 2020

Abstract
Background: To compare the surgical, functional and oncological outcomes between innovatively “three-port” and traditionally “four-port” laparoscopic radical prostatectomy (LRP) in patients with prostate cancer (PCa).Methods: We retrospectively collected the data of PCa patients treated at our institutions from June 2012 to May 2016. According to the inclusion criteria, a total of 234 patients were included in the study, including 112 in group A (four-port) and 122 in group B (three-port). The perioperatively surgical characteristics, functional and oncological outcomes were compared between groups.Results: None of statistical difference in the baseline parameters were detected between these two groups. Compared with group A, the operative time (OT) and estimated blood loss (EBL) were significantly less in group B. The similar follow-up results were obtained in the rate of positive surgical margin (PSM), prostate specific antigen (PSA) biochemical recurrence and continence after LRP without any statistically significant difference. An identical conclusion was also received in comparison of overall survival (OS) and biochemical recurrence-free survival (BRFS) between both groups. Conclusion: Innovatively “three-port” LRP can significantly shorten the OT and reduce the EBL compared with the traditionally “four-port” LRP. Meanwhile, it does not increase the positive rate of PSM and PSA biochemical recurrence. “Three-port” LRP could be popularized in the future in view of its superior surgical technique, considerably functional outcomes and remarkably oncological control.
A novel humanized Frizzled-7-targeting antibody enhances antitumor effects of Bevacizumab against triple-negative breast cancer via blocking Wnt/β-catenin signaling pathway
Authors: Xie Wei , Zhao Huijie , Wang Fengxian , Wang Yiyun , He Yuan , Wang Tong , Zhang Kunchi , Yang Hao , Zhou Zhaoli , Shi Haibin , Wang Jin , Huang Gang 
Published: November 2020

Abstract
Background: Anti-angiogenic therapy has been widely applied to the clinical treatment of malignant tumors. However, the efficacy of such treatments has been called into question, especially in triple-negative breast cancer (TNBC). Bevacizumab, the first anti-angiogenic agent approved by FDA, actually increases invasive and metastatic properties of TNBC cells, resulting from the activation of Wnt/β-catenin signaling in response to hypoxia. As a critical receptor of Wnt/β-catenin signaling, Frizzled-7 (Fzd7) is aberrantly expressed in TNBC, indicating Fzd7 a potential target for developing drugs to be combined with anti-angiogenic agents. Methods: Hybridoma technique and antibody humanization technique were utilized to generate a Fzd7-targeting antibody (SHH002-hu1). Biolayer interferometry (BLI) assay and near infrared (NIR) imaging were conducted to detect the affinity and targeting ability of SHH002-hu1. Next, whether SHH002-hu1 could suppress the invasion and migration of TNBC cells induced by Bevacizumab were validated, and the underlying molecular mechanisms were elucidated by luciferase reporter and western blot assays. The nude-mice transplanted TNBC models were established to assess the anti-TNBC activities of SHH002-hu1 when combined with Bevacizumab. Then, the effects on putative TNBC stem-like cells and Wnt/β-catenin signaling were evaluated by immunofluorescence (IF). Further, the tumor-initiating and self-renew capacity of TNBC cells were studied by secondary nude mouse xenograft model and sphere formation assay. In addition, the effects of SHH002-hu1 on the adaptation of TNBC cells to hypoxia were evaluated by the detection of vasculogenic mimicry (VM) and hypoxia-inducible factor-1α (HIF-1α) transcriptional activity.Results: The novel humanized antibody targeting Fzd7 (SHH002-hu1) exhibited extremely high affinity with Fzd7, and specifically targeted to Fzd7+ cells and tumor tissues. SHH002-hu1 repressed invasion, migration and epithelial-mesenchymal cell transformation (EMT) of TNBC cells induced by Bevacizumab through abating Wnt/β-catenin signaling. SHH002-hu1 significantly enhanced the capacity of Bevacizumab to inhibit the growth of TNBC via reducing the subpopulation of putative TNBC stem-like cells, further attenuating Bevacizumab-enhanced tumor-initiating and self-renew capacity of TNBC cells. Moreover, SHH002-hu1 effectively restrained the adaptation of TNBC cells to hypoxia via disrupting Wnt/β-catenin signaling. Conclusion: SHH002-hu1 significantly enhances the anti-TNBC capacity of Bevacizumab, and shows the potential of preventing TNBC recurrence, suggesting SHH002-hu1 a good candidate for the synergistic therapy together with Bevacizumab.
A Longitudinal Network Analysis of the German Knowledge Economy from 2009 to 2019: Spatio-Temporal Dynamics at the City–Firm Nexus
Authors: Zöllner Silke , Lüthi Stefan , Thierstein Alain 
Published: November 2020

Abstract
Multi-location knowledge-intensive firms span their value chains and thus their locations across space. Increased globalization alters the spatial configuration of such networks of knowledge creation. Longitudinal social network analysis allows detecting temporal changes in the arrangement of nodes and edges in the network and resulting changes in the overall structure. We use this approach to study for Germany the spatio-temporal dynamics of knowledge-intensive services firms – advanced producer services (APS) – in the years between 2009 and 2019. Multi-location APS firms are considered as vanguard of spatial structural change and thus lending to study their location choice behavior. A common approach is to analyze a one-mode intercity network where cities are the nodes. We take a different approach and include the firms’ perspectives. We work directly with the original data structure of a two-mode network including cities and firms as two node sets and we apply stochastic actor-oriented models for network dynamics. Results show that the spatio-temporal dynamics are characterized by both agglomeration and network economies. On a local scale, APS firms continue their location expansion over time and concentrate in agglomerations where many other APS firms and a greater availability of workforce are present. Simultaneously, they also choose new locations in agglomerations further apart from their present locations. On a supra-local scale, the network grows denser over time. Agglomerations that are attractive for APS firms in 2009 become even more attractive in 2019. Our analysis contributes to an understanding of how interactions amongst cities and firms on a local scale give rise to the empirically observed network patterns on a supra-local scale.
Czy proporcja udziału ziarniaków z warstwą i bez warstwy subaleuronowej determinuje poziom i jakość białka w plonie jęczmienia?
Authors: Macewicz Jerzy 
Published: November 2020

Wysokobiałkowe odmiany jęczmienia mogą różnić się zawartością białka w plonie nawet o 50%. Stwierdzamy tę między odmianową zmienność, mimo że warstwa aleuronowa, gromadząca białka zapasowe ma jednakową wielkość w ziarniakach wszystkich odmian. Dotąd brak było morfologicznej interpretacji, wskazującej gdzie w ziarniakach gromadzona jest ta dodatkowa ilość białka, którą wyróżniają się odmiany wysokobiałkowe. Wykonano porównawcze obserwacje struktury endospermu w odmianie najwyżej- i najniżej białkowej. Autorzy barwili białka własną metodą histochemiczną: na powierzchni przełomu nie utrwalonego endospermu. Dzięki zastosowaniu tej prostej metody, stały się możliwe badania porównawcze dużej ilości pojedynczych ziarniaków. Wykazały one, że u części ziarniaków każdej rośliny wysokobiałkowej występuje specyficzna tkanka subaleuronowa. W obrazach mikroskopowych widoczna jest ona między skrobią endospermu a warstwą aleuronową i nie występuje w pozostałych ziarniakach tej samej rośliny. Zatem w niektórych ziarniakach białko gromadzone jest nie tylko w warstwie aleuronowej ale dodatkowo także w tkance subaleuronowej endospermu. Obecność białka subaleuronowego w części ziarniaków rośliny, zwiększa zawartość białka w plonie z tej rośliny czyniąc ją wysokobiałkową. U pozostałych roślin, ziarniaki wysokobiałkowe występują z niższą częstością lub nie występują wcale . rośliny te są niskobiałkowe. Udowodniliśmy w ten sposób, że wysokobiałkowe odmiany jęczmienia wyróżniają się zwiększonym udziałem ziarniaków wysokobiałkowych, zawierających obie tkanki gromadzące białka zapasowe: aleuronową i subaleuronową. Potwierdzeniem uzyskanego wyniku jest 100%-wy udział ziarniaków z tkanką subaleuronową we wszystkich roślinach etiopskiej formy jęczmienia - Hiproly.
Ponadto celem pracy jest wykazanie, że ziarniaki zbierane z pojedynczej rośliny różnią się nie tylko ilością białek, ale i ich jakością. Podjęto w tym celu próbę identyfikacji białek obecnych w obu typach ziarniaków stosując metody analityki biochemicznej. We frakcji białek wydzielonej z ziarniaków zawierających tkankę subaleuronową (wysokobiałkowych), w porównaniu do analogicznej frakcji białek wyizolowanej z ziarniaków bez tej tkanki (niskobiałkowych), stwierdzono znacznie silniejszą obecność kilku peptydów o masach cząsteczkowych w przedziale od 41 kDa do 34 kDa. Przypuszcza się, że są to izoformy endo-B-1, 4-ksylanazy.
Bioethical Aspects of Human Geneticization
Authors: Leźnicki Marcin 
Published: November 2020

Human geneticization, discussed in the following text, is usually associated with the bio-medical practices aimed at human improvement, with the use of genetic enhancement, also understood as genetic improvement or genetic correction. Despite the wide range of benefits claimed by scientists (including biotechnologists and geneticists), these practices undoubtedly generate a number of ethical and legal problems. They concern, among other things, the legitimacy of conducting research in the field of biotechnology or genetics, including reprogenetics, its possible direction, projections related to the development of the research, as well as the methods and means used to control the aforementioned research, or even the problem of both the legislative and moral validity of its further practical implementation. This can be seen, for example, in the ongoing discussion on the geneticization of human life, and in the broader context of its progressive biomedicalization. In view of the considerable breadth and complexity of the issue of geneticization, this text focuses primarily on a closer examination of the possibilities that genetic enhancements, supported by the potential which genetic engineering might bring, followed by due consideration of selected bio-ethical dilemmas that may arise from the application of such enhancements.
Identification of Major Compounds and α-Amylase and α-Glucosidase Inhibitory Activity of Rhizome of Musa balbisiana Colla: An in-vitro and in-silico Study
Authors: Swargiary Ananta , Daimari Manita 
Published: November 2020

Background:α-Amylase and α-glucosidase inhibitors are widely used to suppress postprandial glycemia in the treatment of type-2 diabetes.Objectives:To evaluate the metallic content, phytocompounds, and α-amylase and α-glucosidase inhibitory activity of Musa balbisiana rhizome using in-vitro and in-silico methods.Materials and Methods:Heavy metal content was detected by AAS following standard protocol. Major phytochemicals of the plant were analyzed by GC-MS technique. Enzyme inhibition study was carried out by UV/VIS spectrophotometric methods. The drug-likeness and bio-availability properties of major compounds were carried out using computer-aided tools – SwissADME and ADMElab. Docking and visualization were performed in AutoDock vina and Discovery studio tools.Results:The study found that the fruits of M. balbisiana contain a negligible amount of toxic elements. GC-MS analysis showed five major compounds from the rhizome of M. balbisiana. Invitro enzyme assays revealed strong α-amylase and α-glucosidase inhibitory properties of the plant. All five compounds were predicted to have a drug-likeness property with high cell membrane permeability and bio-availability. The compounds were also predicted to have low to moderate toxicity properties. The Docking study showed strong binding affinities of plant compounds with α-amylase and α-glucosidase. Out of five compounds, C5 showed the best binding affinity with active pockets of α-amylase and α-glucosidase.Conclusion:The in-vitro and in-silico study suggests the antihyperglycemic property of the rhizome of Musa balbisiana and a possible candidate for the therapeutic antidiabetic agent(s).
A Tailored Web- and Text-Based Intervention to Increase Physical Activity for Latino Men: Protocol for a Randomized Controlled Feasibility Trial
Authors: Gans Kim M , Dulin Akilah , Palomo Vanessa , Benitez Tanya , Dunsiger Shira , Dionne Laura , Champion Gregory , Edgar Rachelle , Marcus Bess 
Published: November 2020

Background
Latino men in the United States report low physical activity (PA) levels and related health conditions (eg, diabetes and obesity). Engaging in regular PA can reduce the risk of chronic diseases and yield many health benefits; however, there is a paucity of interventions developed exclusively for Latino men.

Objective
To address the need for culturally relevant PA interventions, this study aims to develop and evaluate Hombres Saludables, a 6-month theory-based, tailored web- and text message-based PA intervention in Spanish for Latino men. This protocol paper describes the study design, intervention, and evaluation methods for Hombres Saludables.

Methods
Latino men aged 18-65 years were randomized to either the individually tailored PA internet intervention arm or the nutrition and wellness internet control arm. The PA intervention included 2 check-in phone calls; automated SMS text messages; a pedometer; a 6-month gym membership; access to a private Facebook group; and an interactive website with PA tracking, goal setting, and individually tailored PA content. The primary outcomes were feasibility, acceptability, and efficacy (minutes per week of total moderate-to-vigorous PA assessed via the ActiGraph GT3X+ accelerometer worn at the waist and 7-day physical activity recall at baseline and 6 months). Secondary outcomes examined potential moderators (eg, demographics, acculturation, and environmental variables) and mediators (eg, self-efficacy and cognitive and behavioral processes of change) of treatment effects at 6 months post randomization.

Results
This study was funded in September 2016. Initial institutional review board approval was received in February 2017, and focus groups and intervention development were conducted from April 2017 to January 2018. Recruitment for the clinical trial was carried out from February 2018 to July 2019. Baseline data collection was carried out from February 2018 to October 2019, with a total of 43 participants randomized. Follow-up data were collected through April 2020. Data cleaning and analysis are ongoing.

Conclusions
We developed and tested protocols for a highly accessible, culturally and linguistically relevant, theory-driven PA intervention for Latino men. Hombres Saludables used an innovative, interactive, web- and text message–based intervention for improving PA among Latino men, an underserved population at risk of low PA and related chronic disease. If the intervention demonstrates feasibility, acceptability, and preliminary efficacy, we will refine and evaluate it in a larger randomized control trial.

Trial Registration
Clinicaltrials.gov: NCT03196570; https://clinicaltrials.gov/ct2/show/NCT03196570

International Registered Report Identifier (IRRID)
DERR1-10.2196/23690
Technology-Supported Guidance Models Stimulating the Development of Critical Thinking in Clinical Practice: Protocol for a Mixed Methods Systematic Review
Authors: Zlamal Jaroslav , Gjevjon Edith Roth , Fossum Mariann , Solberg Marianne Trygg , Steindal Simen Alexander , Strandell-Laine Camilla , Larsen Marie Hamilton , Pettersen Fredrik Solvang , Nes Andréa Aparecida Gonçalves 
Published: November 2020

BackgroundCritical thinking is an essential skill that nursing students need to develop. Technological tools have opened new avenues for technology-supported guidance models, but the challenges and facilitators of such guidance models, as well as how they stimulate the development of critical thinking, remain unclear.ObjectiveWe developed a protocol for a mixed methods systematic review to investigate the use of technology-supported guidance models that stimulate the development of critical thinking in nursing education clinical practice.MethodsA convergent integrated design following the Joanna Briggs Institute Manual for Evidence Synthesis will be employed. A pair of authors will select the articles by screening titles and abstracts, and the methodological quality of the articles included in the review will be assessed by a pair of authors according to checklists for specific study designs. The data will be extracted using the standardized Joanna Briggs Institute mixed methods data extraction form and following a convergent integrated approach. The thematic synthesis for data transformation will be used.ResultsDevelopment of a comprehensive systematic search strategy was completed in October 2020. The database searches were performed on October 21, 2020. As of January 2021, analysis and synthesis is ongoing. Completion of this review is expected by January 2021.ConclusionsBy combining evidence from studies with varied methodological approaches, the results should provide broad insight into the use of technology-supported guidance models for clinical practice in nursing education with a focus on the development of nursing students’ critical thinking. The results of this mixed methods systematic review can also be used to develop or improve current technology-supported guidance models for clinical practice in nursing education.International Registered Report Identifier (IRRID)PRR1-10.2196/25126
Analisis Kesesuaian Lahan Permukiman di Kecamatan Playen Kabupaten Gunungkidul menggunakan Pendekatan Analytic Hierarchy Process
Authors: Ratnawati Hera , Djojomartono, Ph.D. Prijono Nugroho 
Published: November 2020

Peran Kecamatan Playen sebagai kecamatan dengan pertumbuhan ekonomi yang tinggi di Kabupaten Gunungkidul berpengaruh terhadap peningkatan kepadatan penduduk yang ada pada daerah tersebut setiap tahunnya. Kepadatan penduduk yang semakin meningkat dari tahun ke tahun menyebabkan kebutuhan akan ruang juga semakin meningkat, yang berdampak pada terjadinya alih fungsi lahan menjadi lahan permukiman. Penelitian ini bertujuan untuk melakukan analisis kesesuaian lahan permukiman di Kecamatan Playen Kabupaten Gunungkidul menggunakan metode Analytic Hierarchy Proces. Data yang digunakan dalam penelitian ini yaitu berupa data parameter AHP, Peta RDTR Kecamatan Playen dengan Peraturan Daerah Nomor 6 Tahun 2011 dan update data yang dilakukan pada tahun 2018, serta data administrasi berupa batas desa dan batas kecamatan Playen. Data parameter AHP terdiri dari kerawanan bencana, kemiringan lereng, penggunaan lahan, hidrogeologi, jarak terhadap jalan, jenis tanah dan curah hujan. Kesesuaian lahan permukiman diperoleh dengan metode Analytic Hierarchy Process. Proses yang dilakukan yaitu penghitungan AHP dengan menggunakan software Expert Choice 11, pembobotan terhadap masing-masing data parameter, untuk selanjutnya ditampalkan terhadap data administrasi Kecamatan Playen. Hasil akhir dalam penelitian ini yaitu tingkat kesesuaian lahan permukiman di Kecamatan Playen, yaitu S1 (sesuai), S2 (agak sesuai), S3 (kurang sesuai), N1 (tidak sesuai untuk saat ini), dan N2 (tidak sesuai permanen), dengan masing-masing luasan 1.771 ha dan proporsi 16,974 %; 3.865 ha dan proporsi 37,042 %,; 2.647 ha dan proporsi 25,367 %,; 1.893 ha dan proporsi 18,144 %; dan 258 ha dan proporsi 2,472 %.