bluuebunny/crossref_metadata_2025_split huggingface dataset:
Perspectives on the Intracellular Bacterium Chlamydia pneumoniae in Late-Onset Dementia
Authors: Balin Brian J. , Hudson Alan P. 
Published: July 2020

Abstract
Purpose of Review
Chronic diseases remain a daunting challenge for clinicians and researchers alike. While difficult to completely understand, most chronic diseases, including late-onset dementias, are thought to arise as an interplay between host genetic factors and environmental insults. One of the most diverse and ubiquitous environmental insults centers on infectious agents. Associations of infectious agents with late-onset dementia have taken on heightened importance, including our investigations of infection by the intracellular respiratory bacterium, Chlamydia pneumoniae (Cpn), in late-onset dementia of the Alzheimer’s type.

Recent Findings
Over the last two decades, the relationship of this infection to pathogenesis in late-onset dementia has become much clearer. This clarity has resulted from applying contemporary molecular genetic, biochemical, immunochemical, and cell culture techniques to analysis of human brains, animal models, and relevant in vitro cell culture systems. Data from these studies, taken in aggregate form, now can be applied to evaluation of proof of concept for causation of this infection with late-onset disease. In this evaluation, modifications to the original Koch postulates can be useful for elucidating causation.

Summary
All such relevant studies are outlined and summarized in this review, and they demonstrate the utility of applying modified Koch postulates to the etiology of late-onset dementia of the Alzheimer’s type. Regardless, it is clear that even with strong observational evidence, in combination with application of modifications of Koch’s postulates, we will not be able to conclusively state that Cpn infection is causative for disease pathogenesis in late-onset dementia. Moreover, this conclusion obtains as well for the putative causation of this condition by other pathogens, including herpes simplex virus type 1, Borrelia burgdorferi, and Porphyromonas gingivalis.
Effects of Improvisation Training on Student Teachers’ Behavioral, Neuroendocrine, and Psychophysiological Responses during the Trier Social Stress Test
Authors: Seppänen Sirke , Toivanen Tapio , Makkonen Tommi , Jääskeläinen Iiro P. , Anttonen Mikko , Tiippana Kaisa 
Published: July 2020

Abstract
Objectives
Teaching involves multiple performance situations, potentially causing psychosocial stress. Since the theater-based improvisation method is associated with diminished social stress, we investigated whether improvisation lessened student teachers’ stress responses using the Trier Social Stress Test (TSST; preparatory phase, public speech, and math task). Moreover, we studied the influence of interpersonal confidence (IC) – the belief regarding one’s capability related to effective social interactions – on stress responses.

Methods
The intervention group (n = 19) received a 7-week (17.5 h) improvisation training, preceded and followed by the TSST. We evaluated experienced stress using a self-report scale, while physiological stress was assessed before (silent 30-s waiting period) and during the TSST tasks using cardiovascular measures (heart rate, heart rate variability [HRV]), electrodermal activation, facial electromyography (f-EMG), and EEG asymmetry. Hypothalamus-pituitary-adrenal (HPA-axis) reactivity was assessed through repeated salivary cortisol sampling.

Results
Compared to the control group (n = 16), the intervention group exhibited less f-EMG activity before a public speech and higher HRV before the math task. The low IC intervention subgroup reported significantly less stress during the math task. The controls showed a decreased heart rate before the math task, and controls with a low IC exhibited higher HRV during the speech. Self-reported stress and cortisol levels were positively correlated during the post-TSST preparatory phase.

Conclusions
These findings suggest that improvisation training might diminish stress levels, specifically before a performance. In addition, interpersonal confidence appears to reduce stress responses. The decreased stress responses in the control group suggest adaptation through repetition.
DUALIZING INVOLUTIONS ON THE METAPLECTIC GL(2) à la TUPAN
Authors: BALASUBRAMANIAN KUMAR , TIWARI EKTA 
Published: July 2020

AbstractLet F be a non-Archimedean local field of characteristic zero. Let G = GL(2, F) and
$3\widetildeG = \widetilde{GL}(2,F)$
be the metaplectic group. Let τ be the standard involution on G. A well-known theorem of Gelfand and Kazhdan says that the standard involution takes any irreducible admissible representation of G to its contragredient. In such a case, we say that τ is a dualizing involution. In this paper, we make some modifications and adapt a topological argument of Tupan to the metaplectic group
$\widetildeG$
and give an elementary proof that any lift of the standard involution to
$\widetildeG$
; is also a dualizing involution.
A cross-language study on feedforward and feedback control of voice intensity in Chinese–English bilinguals
Authors: Cai Xiao , Yin Yulong , Zhang Qingfang 
Published: July 2020

AbstractSpeech production requires the combined efforts of feedforward and feedback control, but it remains unclear whether the relative weighting of feedforward and feedback control is organized differently between the first language (L1) and the second language (L2). In the present study, a group of Chinese–English bilinguals named pictures in their L1 and L2, while being exposed to multitalker noise. Experiment 1 compared feedforward control between L1 and L2 speech production by examining intensity increases in response to a masking noise (90 dB SPL). Experiment 2 compared feedback control between L1 and L2 speech production by examining intensity increases in response to a weak (30 dB SPL) or strong noise (60 dB SPL). We also examined a potential relationship between L2 fluency and the relative weighting of feedforward and feedback systems. The results indicated that L2 speech production relies less on feedforward control relative to L1, exhibiting attenuated Lombard effects to the masking noise. In contrast, L2 speech production relies more on feedback control than L1, producing larger Lombard effects to the weak and strong noise. The relative weighting of feedforward and feedback control is dynamically changed as second language learning progresses.
Viability and vigour loss during storage of Rudbeckia mollis seeds having different mass: an intra-specific perspective
Authors: Genna Nicholas G. , Walters Christina , Pérez Héctor E. 
Published: July 2020

AbstractRecent evidence points to relationships between intra-specific seed mass variation and viability loss in response to ageing stress. However, little is known about how seed quality may change temporally in response to such stress. Here we examined seed–water relations of mass-separated Rudbeckia mollis seeds to better understand physiological status among mass classes. We then evaluated seed viability and vigour changes in response to various storage conditions or post-storage vigour tests (a 41°C, 75% RH stress for up to 45 d). We found similar pre-storage physiology among mass classes. However, seeds of lower mass deteriorated up to 1.5-fold faster than heavier seeds under certain conditions. Stressing seeds after storage resulted in distinct vigour differences among mass classes. For example, vigour in lower mass seeds tended to decline more compared to heavier seeds following storage in a climate-controlled room. Alternatively, vigour loss varied among mass classes following storage in a non-climate-controlled shed. Our results highlight the importance of distinguishing between pre-sowing storage and post-storage vigour effects when quantifying relative levels of viability loss among seeds of different mass. Furthermore, differential responses to storage and ageing stress among mass classes may have important implications for post-storage regeneration and subsequent population dynamics.
Low intakes of vitamins C and A are associated with obesity in early adulthood
Authors: Correa-Rodríguez María , Luis Gómez-Urquiza Jose , Medina-Martínez Irene , González-Jiménez Emilio , Schmidt-RioValle Jaqueline , Rueda-Medina Blanca 
Published: July 2020

Abstract. To evaluate the relationships between the intake of individual antioxidants as well as the dietary antioxidant quality score and obesity-related measures. A cross-sectional study was conducted on 562 young adults. Fat mass, fat-mass percentage, and fat-free mass were measured using a body composition analyzer. The intake of antioxidant nutrients including vitamins C, E, and A, selenium, zinc, and magnesium were calculated based on a 72-hour diet recall interview. We observed significant differences in the vitamin C (88.6 ± 72.6 mg/day vs. 70.7 ± 60.6 mg/day, p = 0.010), vitamin A (635.8 ± 519.8 μg/day vs. 492.6 ± 318.9 μg/day, p = 0.014), and selenium (135.3 ± 88.7 μg/day vs. 139.3 ± 79.3 μg/day, p = 0.034) intake between normal-weight and overweight or obese young adults. When the Dietary Antioxidant Quality Score (DAQS) was analyzed, there were no significant differences between normal-weight versus overweight or obese young adults after adjusting for confounders. Logistic regression analysis revealed that vitamin C intake (odds ratio (OR) 0.995, 95% CI 0.992–0.999, p = 0.013) and vitamin A intake (OR 0.999, 95% CI 0.999–1.000, p = 0.016) were independent predictors of overweight/obesity after adjusting for age, sex and energy intake. In contrast, a higher selenium intake was associated with an increased risk of overweight/obesity (OR 1.003, 95% CI 1.000–1.006, p = 0.034). Future longitudinal investigations of dietary antioxidant intake in relation to the development of obesity would be of interest to better understand the effect of dietary antioxidants on obesity.
Layering Structures in the Porous Material Beneath the Chang'e‐3 Landing Site
Authors: Ding Chunyu , Li Chunlai , Xiao Zhiyong , Su Yan , Xing Shuguo , Wang Yichen , Feng Jianqing , Dai Shun , Xiao Yuan , Yao Meijuan 
Published: July 2020

AbstractThe lunar penetrating radar (LPR) onboard the Chinese Chang'e‐3 (CE‐3) mission obtained high‐resolution profile data for the continuous ejecta deposits of the Ziwei crater. Geological background suggests that the continuous ejecta deposits contain few large boulders, and the ejecta deposits were largely originated from the pre‐impact regolith. Using the top ~50 ns of radar data, we estimate the bulk density and porosity for the ejecta deposits based on hyperbolic echo patterns in the radargram that are caused by subsurface boulders. The physical properties are close to those of typical lunar regolith. Numerous subparallel and discontinuous short layers are visible in the radargram of the continuous ejecta deposits. The dielectric coefficients of the layering structures are estimated, and their permittivity is slightly larger than that of typical lunar regolith and less than that of basaltic rocks. Cratering physics together with the geological context of this area suggest that the layering structures are most likely ground gravels and/or melt‐welded breccias that were sheared due to the horizontal momentum of the impact ejecta. This interpretation is indicative of the origin of the enigmatic layering structures in regolith core samples returned by the Apollo and Luna missions. The results also highlight the importance of ejecta emplacement in shaping the structure of lunar regolith.
Ionospheric F Layer Scintillation Weakening as Observed by COSMIC/FORMOSAT‐3 During the Major Sudden Stratospheric Warming in January 2013
Authors: Yu Tao , Ye Hailun , Liu Huixin , Xia Chunliang , Zuo Xiaomin , Yan Xiangxiang , Yang Na , Sun Yangyi , Zhao Biqiang 
Published: July 2020

AbstractSudden stratospheric warming (SSW) events in Northern Hemisphere winter atmosphere play a significant effect on large ionospheric disturbances. Nevertheless, the SSW effects on small‐scale structure in ionosphere, that is, the ionospheric F layer irregularities, are generally lack of understanding. This work carefully studied the equatorial F layer scintillation during the SSW event extending from 4 January to 5 February in 2013 by using the data of COSMIC/FORMOSAT‐3 (a Constellation Observing System for Meteorology, Ionosphere, and Climate mission). The occurrence rate of ionospheric F layer scintillation was found to be apparently decreased by about 30% during the SSW period. This phenomenon was obviously seen in American sector (90°W–0°) and African sector (0°–90°E). Another prominent feature was the ~0.5 local time hour delay of maximum scintillation occurrence rate associated with the suppression of equatorial F layer irregularities in the first 2 hr after the sunset during the SSW event in January 2013.
Association of progression-free or event-free survival with overall survival in diffuse large B-cell lymphoma after immunochemotherapy: a systematic review
Authors: Zhu Jie , Yang Yong , Tao Jin , Wang Shu-Lian , Chen Bo , Dai Jian-Rong , Hu Chen , Qi Shu-Nan , Li Ye-Xiong 
Published: July 2020

AbstractTo investigate progression-free survival (PFS) and event-free survival (EFS) as early efficacy endpoints in diffuse large B-cell lymphoma (DLBCL), this systematic review included phase III randomized controlled trials (RCTs), phase II trials, and retrospective studies in newly diagnosed DLBCL receiving rituximab-containing chemotherapy through databases search up to 2019. Quality control was performed, where studies with high risk of bias were excluded. Prediction models were first established using the RCTs, and then externally validated in the phase II and retrospective populations. Trial-level surrogacy analysis was conducted by correlating the logarithmic (log) hazard ratio (HR) for PFS or EFS and log HR for OS. Correlation analysis at treatment arm-level was performed between 1-, 2-, 3-, and 5-year PFS or EFS rates and 5-year OS. The correlation was evaluated using the Pearson correlation coefficientrin weighted linear regression, with weight equal to patient size. Sensitivity analyses were performed to assess the consistency of predictive model by leaving one subgroup of trials out at a time. Twenty-six phase III RCTs, 4 phase II trials and 47 retrospective studies were included. In trial-level surrogacy, PFS (r, 0.772; 95% confidence interval [CI], 0.471–0.913) or EFS (r, 0.838; 95% CI, 0.625–0.938) were associated with OS. For rituximab immunochemotherapy treatment arms in RCTs, there was a linear correlation between 1 and 5-year PFS (r, 0.813–0.873) or EFS (r, 0.853–0.931) and 5-year OS. Sensitivity analysis demonstrated reasonable overall consistency. The correlation between PFS and OS was externally validated using independent phase II, and retrospective data (r, 0.795–0.897). We recommend PFS and EFS as earlier efficacy endpoints in patients with DLBCL primarily treated with rituximab-containing immunochemotherapy.
NMDAR-mediated modulation of gap junction circuit regulates olfactory learning in C. elegans
Authors: Choi Myung-Kyu , Liu He , Wu Taihong , Yang Wenxing , Zhang Yun 
Published: July 2020

AbstractModulation of gap junction-mediated electrical synapses is a common form of neural plasticity. However, the behavioral consequence of the modulation and the underlying molecular cellular mechanisms are not understood. Here, using a C. elegans circuit of interneurons that are connected by gap junctions, we show that modulation of the gap junctions facilitates olfactory learning. Learning experience weakens the gap junctions and induces a repulsive sensory response to the training odorants, which together decouple the responses of the interneurons to the training odorants to generate learned olfactory behavior. The weakening of the gap junctions results from downregulation of the abundance of a gap junction molecule, which is regulated by cell-autonomous function of the worm homologs of a NMDAR subunit and CaMKII. Thus, our findings identify the function of a gap junction modulation in an in vivo model of learning and a conserved regulatory pathway underlying the modulation.
Water rights shape crop yield and revenue volatility tradeoffs for adaptation in snow dependent systems
Authors: Malek Keyvan , Reed Patrick , Adam Jennifer , Karimi Tina , Brady Michael 
Published: July 2020

AbstractIrrigated agriculture in snow-dependent regions contributes significantly to global food production. This study quantifies the impacts of climate change on irrigated agriculture in the snow-dependent Yakima River Basin (YRB) in the Pacific Northwest United States. Here we show that increasingly severe droughts and temperature driven reductions in growing season significantly reduces expected annual agricultural productivity. The overall reduction in mean annual productivity also dampens interannual yield variability, limiting yield-driven revenue fluctuations. Our findings show that farmers who adapt to climate change by planting improved crop varieties may potentially increase their expected mean annaul productivity in an altered climate, but remain strongly vulnerable to irrigation water shortages that substantially increase interannual yield variability (i.e., increasing revenue volatility). Our results underscore the importance for crop adaptation strategies to simultaneously capture the biophysical effects of warming as well as the institutional controls on water availability.
Structure of the DOCK2−ELMO1 complex provides insights into regulation of the auto-inhibited state
Authors: Chang Leifu , Yang Jing , Jo Chang Hwa , Boland Andreas , Zhang Ziguo , McLaughlin Stephen H. , Abu-Thuraia Afnan , Killoran Ryan C. , Smith Matthew J. , Côté Jean-Francois , Barford David 
Published: July 2020

AbstractDOCK (dedicator of cytokinesis) proteins are multidomain guanine nucleotide exchange factors (GEFs) for RHO GTPases that regulate intracellular actin dynamics. DOCK proteins share catalytic (DOCKDHR2) and membrane-associated (DOCKDHR1) domains. The structurally-related DOCK1 and DOCK2 GEFs are specific for RAC, and require ELMO (engulfment and cell motility) proteins for function. The N-terminal RAS-binding domain (RBD) of ELMO (ELMORBD) interacts with RHOG to modulate DOCK1/2 activity. Here, we determine the cryo-EM structures of DOCK2−ELMO1 alone, and as a ternary complex with RAC1, together with the crystal structure of a RHOG−ELMO2RBD complex. The binary DOCK2−ELMO1 complex adopts a closed, auto-inhibited conformation. Relief of auto-inhibition to an active, open state, due to a conformational change of the ELMO1 subunit, exposes binding sites for RAC1 on DOCK2DHR2, and RHOG and BAI GPCRs on ELMO1. Our structure explains how up-stream effectors, including DOCK2 and ELMO1 phosphorylation, destabilise the auto-inhibited state to promote an active GEF.
Searching large-scale scRNA-seq databases via unbiased cell embedding with Cell BLAST
Authors: Cao Zhi-Jie , Wei Lin , Lu Shen , Yang De-Chang , Gao Ge 
Published: July 2020

AbstractSingle-cell RNA-seq (scRNA-seq) is being used widely to resolve cellular heterogeneity. With the rapid accumulation of public scRNA-seq data, an effective and efficient cell-querying method is critical for the utilization of the existing annotations to curate newly sequenced cells. Such a querying method should be based on an accurate cell-to-cell similarity measure, and capable of handling batch effects properly. Herein, we present Cell BLAST, an accurate and robust cell-querying method built on a neural network-based generative model and a customized cell-to-cell similarity metric. Through extensive benchmarks and case studies, we demonstrate the effectiveness of Cell BLAST in annotating discrete cell types and continuous cell differentiation potential, as well as identifying novel cell types. Powered by a well-curated reference database and a user-friendly Web server, Cell BLAST provides the one-stop solution for real-world scRNA-seq cell querying and annotation.
Molecular basis for the distinct functions of redox-active and FeS-transfering glutaredoxins
Authors: Trnka Daniel , Engelke Anna D. , Gellert Manuela , Moseler Anna , Hossain Md Faruq , Lindenberg Tobias T. , Pedroletti Luca , Odermatt Benjamin , de Souza João V. , Bronowska Agnieszka K. , Dick Tobias P. , Mühlenhoff Uli , Meyer Andreas J. , Berndt Carsten , Lillig Christopher Horst 
Published: July 2020

AbstractDespite their very close structural similarity, CxxC/S-type (class I) glutaredoxins (Grxs) act as oxidoreductases, while CGFS-type (class II) Grxs act as FeS cluster transferases. Here we show that the key determinant of Grx function is a distinct loop structure adjacent to the active site. Engineering of a CxxC/S-type Grx with a CGFS-type loop switched its function from oxidoreductase to FeS transferase. Engineering of a CGFS-type Grx with a CxxC/S-type loop abolished FeS transferase activity and activated the oxidative half reaction of the oxidoreductase. The reductive half-reaction, requiring the interaction with a second GSH molecule, was enabled by switching additional residues in the active site. We explain how subtle structural differences, mostly depending on the structure of one particular loop, act in concert to determine Grx function.
The sources and transmission routes of microbial populations throughout a meat processing facility
Authors: Zwirzitz Benjamin , Wetzels Stefanie U. , Dixon Emmanuel D. , Stessl Beatrix , Zaiser Andreas , Rabanser Isabel , Thalguter Sarah , Pinior Beate , Roch Franz-Ferdinand , Strachan Cameron , Zanghellini Jürgen , Dzieciol Monika , Wagner Martin , Selberherr Evelyne 
Published: July 2020

AbstractMicrobial food spoilage is responsible for a considerable amount of waste and can cause food-borne diseases in humans, particularly in immunocompromised individuals and children. Therefore, preventing microbial food spoilage is a major concern for health authorities, regulators, consumers, and the food industry. However, the contamination of food products is difficult to control because there are several potential sources during production, processing, storage, distribution, and consumption, where microorganisms come in contact with the product. Here, we use high-throughput full-length 16S rRNA gene sequencing to provide insights into bacterial community structure throughout a pork-processing plant. Specifically, we investigated what proportion of bacteria on meat are presumptively not animal-associated and are therefore transferred during cutting via personnel, equipment, machines, or the slaughter environment. We then created a facility-specific transmission map of bacterial flow, which predicted previously unknown sources of bacterial contamination. This allowed us to pinpoint specific taxa to particular environmental sources and provide the facility with essential information for targeted disinfection. For example, Moraxella spp., a prominent meat spoilage organism, which was one of the most abundant amplicon sequence variants (ASVs) detected on the meat, was most likely transferred from the gloves of employees, a railing at the classification step, and the polishing tunnel whips. Our results suggest that high-throughput full-length 16S rRNA gene sequencing has great potential in food monitoring applications.