bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identifying no-harm incidents in home healthcare: a cohort study using trigger tool methodology
Authors: Lindblad Marléne , Unbeck Maria , Nilsson Lena , Schildmeijer Kristina , Ekstedt Mirjam 
Published: March 2020

Abstract
Background Patient safety in home healthcare is largely unexplored. No-harm incidents may give valuable information about risk areas and system failures as a source for proactive patient safety work. We hypothesized that it would be feasible to retrospectively identify no-harm incidents and thus aimed to explore the cumulative incidence, preventability, types, and potential contributing causes of no-harm incidents that affected adult patients admitted to home Healthcare. Methods A structured retrospective record review using a trigger tool designed for home healthcare. A random sample of 600 home healthcare records from ten different organizations across Sweden was reviewed. Results In the study, 40,735 days were reviewed. In all, 313 no-harm incidents affected 177 (29.5%) patients; of these, 198 (63.2%) no-harm incidents, in 127 (21.2%) patients, were considered preventable. The most common no-harm incident types were “fall without harm,” “deficiencies in medication management,” and “moderate pain.” The type “deficiencies in medication management” was deemed to have a preventability rate twice as high as those of “fall without harm” and “moderate pain.” The most common potential contributing cause was “deficiencies in nursing care and treatment, i.e., delayed, erroneous, omitted or incomplete treatment or care.” Conclusion This study suggests that it is feasible to identify no-harm incidents and potential contributing causes such as omission of care using record review with a trigger tool adapted to the context. No-harm incidents and potential contributing causes are valuable sources of knowledge for improving patient safety, as they highlight system failures and indicate risks before an adverse event reach the patient.
Build a Bioinformatics Analysis Platform and Apply it to Routine Analysis of Microbial Genomics and Comparative Genomics
Authors: Liu Hualin , Xin Bingyue , Zheng Jinshui , Zhong Hao , Yu Yun , Peng Donghai , Sun Ming 
Published: March 2020

Abstract
Genomics and comparative genomics have been increasingly used as routine methods for general microbiological researches. However, it is usually necessary to call several tools or even write some scripts to complete some simple analysis, which is complicated for most biological researchers. To simplify the operation process, especially for the convenience of microbiologists in the analysis, here we have developed PGCGAP, a comprehensive, malleable and easily-installed prokaryotic genomics and comparative genomics analysis pipeline, which implements genome assembly, gene prediction and annotation, average nucleotide identity (ANI) calculation, phylogenetic analysis, COG annotation, pan-genome analysis, inference of orthologous gene groups, variants calling and annotation and screening for antimicrobial and virulence genes. Although we have tried our best to simplify the installation and usage of PGCGAP, it may be difficult for non-bioinformatician users to master it. So, a protocol was created to help microbiologists without any experience in bioinformatics to establish their own bioinformatics platform and perform routine analysis. This protocol shows how to choose equipment, to install a Linux subsystem on a laptop with windows 10 system, to install PGCGAP and perform all analysis with an example dataset. The protocol requires a basic understanding of Linux, so an additional web page was written to help uninitiated users learn Linux and whole-genome sequencing (https://github.com/liaochenlanruo/pgcgap/wiki/Learning-bioinformatics or http://bcam.hzau.edu.cn/linuxwgs.php).
A Mutant Vesicular Stomatitis Virus with Reduced Cytotoxicity and Enhanced Anterograde Trans-synaptic Efficiency
Authors: Lin Kunzhang , Zhong Xin , Ying Min , Li Lei , Tao Sijue , Zhu Xutao , He Xiaobin , Xu Fuqiang 
Published: March 2020

Abstract
Understanding the connecting structure of brain network is the basis to reveal the principle of the brain function and elucidate the mechanism of brain diseases. Trans-synaptic tracing with neurotropic viruses has become one of the most effective technologies to dissect the neural circuits. Although the retrograde trans-synaptic tracing for analyzing the input neural networks with recombinant rabies and pseudorabies virus has been broadly applied in neuroscience, viral tools for analyzing the output neural networks are still lacking. The recombinant vesicular stomatitis virus (VSV) has been used for the mapping of synaptic outputs. However, several drawbacks, including high neurotoxicity and rapid lethality in experimental animals, hinder its application in long-term studies of the structure and function of neural networks. To overcome these limitations, we generated a recombinant VSV with replication-related N gene mutation, VSV-NR7A, and examined its cytotoxicity and efficiency of trans-synaptic spreading. We found that by comparison with the wild-type tracer of VSV, the NR7A mutation endowed the virus lower rate of propagation and cytotoxicity in vitro, as well as significantly reduced neural inflammatory responses in vivo and much longer animal survival when it was injected into the nucleus of the mice brain. Besides, the spreading of the attenuated VSV was delayed when injected into the VTA. Importantly, with the reduced toxicity and extended animal survival, the number of brain regions that was trans-synaptically labeled by the mutant VSV was more than that of the wild-type VSV. These results indicated that the VSV-NR7A, could be a promising anterograde tracer that enables researchers to explore more downstream connections of a given brain region, and observe the anatomical structure and the function of the downstream circuits over a longer time window. Our work could provide an improved tool for structural and functional studies of neurocircuit.
Genomic Characterization of an Emerging Enterobacteriaceae Species: the First Case of Co-infection With a Typical Pathogen in a Human Patient
Authors: Zhang Zhao , Li Daixi , shi xing , Zhai Yao , Guo Yatao , Zheng Yali , Zhao Lili , He Yukun , Chen Yusheng , Wang Zhanwei , Su Jianrong , Kang Yu , Gao Zhancheng 
Published: March 2020

Abstract
Background Opportunistic pathogens are important for clinical practice as they often cause antibiotic-resistant infections. However, little is documented for many emerging opportunistic pathogens and their biological characteristics. Here, we isolated a novel species strain of extended-spectrum β-lactamase-producing Enterobacteriaceae from a patient of with a biliary tract infection. And we explored the biological and genomic characteristics of this strain to provide new evidence and detailed information for opportunistic pathogens about the co-infection they may cause. Results The isolate grows grew very slowly but confers conferred strong protection for the co-infected cephalosporin-sensitive Klebsiella pneumonia pneumoniae . As the initial laboratory testing failed to identify the taxonomy of the strain, great perplexity was caused in the etiological diagnosis , and anti-infection treatment for the patient. Rigorous sequencing efforts achieved the complete genome sequence of the isolate which we designated as AF18. AF18 is phylogenetically close to a few strains respectively isolated from soil, clinical sewage, and patients, forming a novel species together, while the taxonomic nomenclature of which is still under discussion. And this is the first report of human infection of this novel species. Like As its relatives, AF18 harbors many genes related to cell mobility, various genes adaptive to both natural wild environment and animal host, tens of over 30 mobile genetic mobile elements, and a plasmid bearing bla CTX-M-3 gene, indicating its ability to disseminate antimicrobial - resistant genes from the natural environment to patients. Transcriptome sequencing identified two sRNAs that critically regulate the growth rate of AF18 , which could serve as targets for novel antimicrobial strategies. Conclusions Our findings imply that AF18 and its species are not only infection-relevant but also potential disseminators of antibiotic resistance genes i n transferring antibiotic determinants , which highlights the need for continuous monitoring for this novel species and efforts to develop controlling treatment strategies.
Depreciation analysis and filter model review
Authors: abidi monem 
Published: March 2020

Abstract
Deciding to invest in oneself is not a direct and simple action. It is as complicated as it took a good time to besiege the factors influencing this investment process. In this respect, many approaches have emerged, sometimes contradictory, and complementary in other cases. The introduction of depreciation raises the challenge to pinpoint the problem, which remains so far fierce to any domestication. This paper presents a model that takes into account these different lines (such as the average level of aptitude of the entire population, the degree of homogeneity of skills within the same type of diploma). It turns out that the market value of a diploma tends to zero if the dispersion of skills is equal to productivity multiplied by the average aptitude of this level. A new conception of investment in education is thus formed, in which the price awarded to diplomas depends largely on the ability, whatever the average, or in terms of variance, stopping their investment in training at the same level.
Recombinant Adenovirus Expressing Vesicular Stomatitis Virus G Proteins induce both humoral and cell-medicated immune responses in Mice and Goats
Authors: Xue Xiaojuan , Yu Zhaorong , Jin Hongyan , Liang Lin , Li Jiayang , Li Xiaolu , Wang Yong , Cui Shangjin , Li Gang 
Published: March 2020

Abstract
Background: With a human type 5 replication-defective adenovirus expression vector, we constructed the three recombinant adenoviruses (rAd) and expressed the Vesicular Stomatitis Virus (VSV) Indiana serotype glycoprotein (VSV-IN-G), VSV New Jersey serotype glycoprotein (VSV-NJ-G), and the G fusion protein [two serotypes G (VSV-IN-G-NJ-G)]. Three rAds were named rAd-IN, rAd-NJ, and rAd-IN-NJ. The three rAds were inoculated into AAV-293 cells, and the AAV-293 cells were serially propagated to 20 generations until the virus titers were stable, then TCID50 was determined. In direct immunofluorescence and western blot were used for detecting the expression of the target proteins and lymphocyte proliferation test was used for immune cell numbers. Results: The results showed that G proteins we expressed with good reactogenicity. The rAds were used to subcutaneously inoculate mice three times with 2-week intervals, and goats two times with 3-week intervals, respectively. On 0, 2, 4, and 6 weeks of post-inoculation for the mice and 0, 3, 6, 9, and 12 weeks for goats, their sera were collected and NT antibodies were determined. The results showed that the rAds could induce the production of VSV antibodies in the mice, and VSV NT antibodies in the goats. The antibody levels were 1:16 to 1: 32 in mice, and 1:32 to 1: 64 in the goats. The rAds induced strong immune lymphocyte proliferations in mice and goats, which was significantly higher than those of the negative control groups. Conclusion: The three rAds expressed VSV-G proteins at high levels, and induced humoral and cellular immune responses in both mice and goats, which laid a foundation for further studies of the recombinant adenovirus vaccines expressing VSV glycoprotein.
Molecular confirmation of hemothropic mycoplasmas (hemoplasmas) in domestic cats (Felis catus) in Romania
Authors: Imre Mirela , Văduva Cristina , Dărăbuș Gheorghe , Morariu Sorin , Herman Viorel , Suici Tijana , Lait Philippa , Imre Kálmán 
Published: March 2020

Abstract
Background: The hemotropic mycoplasmas (hemoplasmas) of the genus Mycoplasma are recognized as important bacteria that parasitize red blood cells, causing hemolytic anemia in many mammalian species, including cats. No information is available concerning the presence of feline hemoplasma infections in cats in Romania. Thus, the objective of the present study was to provide data on the occurrence and molecular characterization of hemothropic mycoplasmas in client owned cats in Romania. Methods : Blood samples from 51 unhealthy cats, originating from Timişoara Municipality, Romania, were screened for the presence of hemoplasmas using conventional polymerase chain reaction (PCR) targeting the 16S rRNA gene and sequencing assays. Results: Molecular analysis revealed 11 (21.6%) positive samples, consisting of 8 (72.7%) Candidatus Mycoplasma haemominutum and 3 (27.3%) Mycoplasma haemofelis confirmed positives. Candidatus Mycoplasma turicensis was not detected, and no co-infections were registered. No significant associations ( p > 0 . 05) were found between the hemoplasma infection status and age, gender, breed, presence of ectoparasites, FeLV/FIV positivity of cats or the sampling season. However, outdoor access was positively associated ( p =0.049) with infection and could be considered a risk factor (OR=4.1) in acquiring feline hemotropic mycoplasmas. Conclusions : The findings support the emergence of feline hemoplasma infections in previously uninvestigated territories of Europe, providing useful information for small animal practitioners. To our knowledge, the present survey is the first reported molecular evidence of feline hemoplasma infections in Romania.
Major Leg Wound Complications between the Proximal and Distal Segments of Saphenous Veins Harvested by No-Touch Technique in Coronary Artery Bypass Graft Surgery: study protocol for a single-center randomized within-subject controlled clinical trial
Authors: Tian Meice , Hu Shengshou , Wang Xianqiang , Li Sidong , Wang Liqing 
Published: March 2020

Abstract
Background : The No-Touch saphenous vein harvesting technique has been proved to reduce vein graft occlusion after coronary artery bypass graft surgery. However, the optimum segment of the saphenous vein for this technique is still undetermined. Previous studies implicated lower occlusion of proximal segments of saphenous veins compared to distal segments, but with concern of higher leg wound complications. No randomized trials have been carried out accordingly. This pilot study is a single-center randomized within-subject-controlled tria l, comparing the leg wound complications of proximal segments of saphenous veins harvested by No-Touch versus distal segments. Methods : The study is planning intending to enroll 100 coronary artery bypass graft surgery patients from our institution, with each patient requiring two vein grafts with or without cardiopulmonary bypass. Both proximal and distal segments of saphenous veins will be harvested by No-Touch technique from ipsilateral legs. The proximal vein segments will be randomized to the left or right coronary territory for bypassing. The primary endpoint is major leg wound complications at 6 days postoperatively. Secondary endpoints include major leg wound complications during 3-month follow-up and graft vessel occlusion at 3 months and 1 year. Clinical events include major adverse cardiac or cerebrovascular events and recurrence of angina Discussion : This is the first study assessing the safety and effectiveness between different segments of No-Touch saphenous veins for coronary artery bypass graft surgery, and will help to establish preliminary evidence for decision making of precise vein graft conduit choice. Trial registration : ClinicalTrials.gov: NCT04284956
Tumor resection reduced circulating tumor cell dissemination
Authors: Zeng Chong , Zhang Xiaoli , Yao Jie 
Published: March 2020

Abstract
Background: Cancer is a major public health problem worldwide. Whether to perform tumor resection or non-resection remains controversial face different types. Circulating tumor cells (CTCs) which are classified into three subpopulations epithelial (E-CTC), mesenchymal (M-CTC), and epithelial mesenchymal (E/M-CTC) based on expression of specificity biomarker.Methods: We identified CTCs using CanPatrol CTC enrichment technique from peripheral blood samples of 930 patients with tumor resection or non-resection. Patients were divided into two groups: Resection (n=615) and Non-resection (n=315). The relationship between tumor resection and count of CTCs were studied by using correlation analysis.Results: The mean patient age was 57.52 years (range, 17–87 years). Univariate analysis tumor resection could significantly reduce the number of total CTC cells (hazard ratio，HR, 0.92, 95% confidence interval, CI= 0.903-0.937; P < 0.001), the E-CTC cells (HR, 0.889, 95% CI= 0.837-0.944; P < 0.001), the M-CTC cells (HR, 0.799, 95% CI= 0.735-0.869; P < 0.001), and the E/M-CTC cells (HR, 0.908, 95% CI= 0.888-0.928; P < 0.001).Furthermore, correlation analysis between total CTC and E-CTC, M-CTC, and E/M-CTC, the pearson correlation coefficient was 0.467, 0.458, and 0.963, respectively.Conclusions: In conclusion，the tumor resection can reduce the number of CTCs in peripheral blood.
Percutaneous vertebroplasty for Kummell’s disease: Short-term analysis of therapeutic effects and outcome predictors
Authors: Li Yibing , Gao Wenjie , Wang Xiaodong , Hao Dingjun 
Published: March 2020

Abstract
Background: To retrospectively investigate the short-term therapeutic effect of percutaneous vertebroplasty (PVP) for Kummell’s disease using a variable angle needle and to analyze the possible outcome predictors.Methods: Twenty-seven patients were included in this study. Medical records were evaluated. The following factors were analyzed on imaging data: location of lesion, the percentage of bone marrow edema, patterns of cement spreading, and post-procedural kyphosis correction. Visual analogue score (VAS) was used to analyze the clinical effect, and the improvement rate was calculated. Statistical analyses were conducted to evaluate the relationship between those factors and therapeutic outcomes using Fisher’s exact test, Chi-squared test, and Student’s unpaired t-test.Results: The effectiveness rate was 70.4% at 1 week, 74.1% after 1 month, and 63.0% after 3 months. Six months and one year after procedures, the effective rate remained steady at 70.4%. Post-procedural kyphosis correction ≥8° was a risk factor for inferior pain relief. An area of edema over 40% was a prognostic factor for superior pain relief at the first follow-up point. Cement morphology was a positive predictor for better pain relief at 1 month follow-up.Conclusions: Compared to the therapeutic effects of PVP on fresh osteoporotic vertebral compression fractures (OVCF), percutaneous vertebroplasty for Kummell’s disease was effective in only 63% to 74.1% of the patients at different follow-up points. Overemphasized kyphotic correction (over 8 degrees) was associated with poor outcomes. Areas of edema over 40% and cement morphology of mixed type were positive prognostic factors in the early stage.
IgG4-related aortitis/periaortitis and periarteritis: a distinct spectrum of IgG4-related disease
Authors: Peng Linyi , Zhang Panpan , Li Jieqiong , Liu Zheng , Lu Hui , Zhu Liang , Wang Xiaorong , Teng Fei , Li Xuemei , Guo Huifang , Fei Yunyun , Zhang Wen , Zhao Yan , Zeng Xiaofeng , Zhang Fengchun 
Published: March 2020

Abstract
Background: Large vessels could be involved in IgG4-related disease(IgG4-RD).This study aimed to clarify the clinical features and evaluate the treatment efficacy for IgG4-RD with aortitis/periaortitis and periarteritis (PAO/PA). Methods: This study enrolled 587 IgG4-RD patients in a prospective cohort with a follow-up time for more than 6 months. The distribution of IgG4-related PAO/PA was classified into four types: type 1, thoracic aorta; type 2a, abdominal aorta; type 2b, abdominal aorta and iliac artery; type 2c, iliac artery; type 3, thoracic and abdominal aorta; type 4, other arteries. Patient’s demographic data, clinical characteristics, laboratory parameters, and treatment efficacy were analyzed. Results: Of 587 IgG4-RD patients, 89(15.2%) had PAO/PA. The average age was 58.3±11.1 years, with male predominance (85.4%). Vessels affected were as follows: abdominal aorta (83.1%), iliac artery (70.8%), thoracic aorta (13.5%) and other vessels (13.5%). The most prevalent distribution type of IgG4-related PAO/PA was type 2b, with 74 (83.1%) patients, followed by type 2a, type 2c, type 3, and type 1. 55 (61.8%) PAO/PA patients had hydronephrosis, with renal insufficiency occurred in 43 (48.3%), and 31 (34.8%) PAO/PA patients had D-J stent drainage due to severe ureteral obstruction. After treatment with glucocorticoid and immunosuppressants, 82% patients achieved a remission with shrinking of perivascular mass. Conclusions: IgG4-RD with PAO/PA was distinct from non-PAO/PA in demographic features, organs involvement distribution, inflammatory markers, serum IgG4 and IgE. The most common affected vessel was abdominal aorta, and most patients responded well with treatment.
The Role of Series Cholecystectomy in High Risk Acute Cholecystitis Patients Who Underwent Gallbladder Drainage
Authors: Wang Chi-Chih , Tseng Ming-Hseng , Lin Chun-Che , Wu Sheng-Wen , Yang Tzu-Wei , Sung Wen-Wei , Wang Yao-Tung , Lee Hsiang-Lin , Shiu Bei-Hao , Tsai Ming-Chang 
Published: March 2020

Abstract
Background Cholecystectomy (CCY) is the only definitive therapy for acute cholecystitis. We conducted this study to evaluate which patients may not benefit from further CCY after percutaneous transhepatic gallbladder drainage (PTGBD) has been performed in acute cholecystitis patients.Methods Acute cholecystitis patients with PTGBD treatment were selected from one million random samples from the National Health Insurance Research Database obtained between January 2004 and December 2010. Recurrent biliary events (RBEs), RBE-related medical costs, RBE-related mortality rate and an RBE-free survival curve were compared in patients who accepted CCY within 2 months and patients without CCY within 2 months after the index admission.Results 365 acute cholecystitis patients underwent PTGBD at the index admission. A total of 190 patients underwent further CCY within 2 months after the index admission. The other 175 patients did not accept further CCY within 2 months after the index admission. RBE-free survival was significantly better in the CCY within 2 months group (60% vs. 42%, p<0.001). The RBE-free survival of the CCY within 2 months group was similar to that of the no CCY within 2 months group in patients ≥ 80 years old and patients with a Charlson Comorbidity Index (CCI) score ≥ 9.Conclusion We confirmed CCY after PTGBD reduced RBEs, RBE-related medical expenses, and the RBE-related mortality rate in patients with acute cholecystitis. In patients who accepted PTGBD, the RBE and survival benefits of CCY within 2 months became insignificant in patients ≥ 80 years old or with a CCI score ≥ 9.