bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cross-sectional and prospective relationship between physical activity and depression symptoms
Authors: Marques Adilson , Bordado Joana , Peralta Miguel , Gouveia Elvio R. , Tesler Riki , Demetriou Yolanda , Gomez Baya Diego 
Published: September 2020

AbstractThis study aimed to analyse the cross-sectional and prospective relationship between moderate and vigorous physical activity (PA) and depression symptoms. This study analysed 32,392 European late middle-aged to older adults, from 14 European countries across a 4-year follow-up. Data was collected in the fourth (in 2011) and sixth (in 2015) wave, from the Survey of Health, Ageing and Retirement in Europe (SHARE). For the present analysis, participants were considered who responded to the EURO-D 12-item scale of depression symptoms and reported the intensity and frequency of PA. ANCOVAs were conducted to assess the cross-sectional and prospective associations. For both men and women, engaging in moderate or vigorous PA in 2011 was associated with a lower score of depression in 2011 and 2015. From the prospective analysis, moderate and vigorous PA in 2011 was inversely associated with the score of depression. This association remains significant in the fully adjusted for self-rated health, sociodemographic characteristics, and the presence of chronic diseases. Moderate and vigorous PA at least once a week is negatively related to the score of depression, both in men and women. PA is negatively associated with depression symptoms, and from prospective analysis PA predicts lower depression scores 4 years later.
Deciphering heterogeneity of septic shock patients using immune functional assays: a proof of concept study
Authors: Albert Vega Chloé , Oriol Guy , Bartolo François , Lopez Jonathan , Pachot Alexandre , Rimmelé Thomas , Venet Fabienne , Leray Véronique , Monneret Guillaume , Delwarde Benjamin , Brengel-Pesce Karen , Textoris Julien , Mallet François , Trouillet-Assant Sophie 
Published: September 2020

AbstractThe complexity of sepsis pathophysiology hinders patient management and therapeutic decisions. In this proof-of-concept study we characterised the underlying host immune response alterations using a standardised immune functional assay (IFA) in order to stratify a sepsis population. In septic shock patients, ex vivo LPS and SEB stimulations modulated, respectively, 5.3% (1/19) and 57.1% (12/21) of the pathways modulated in healthy volunteers (HV), highlighting deeper alterations induced by LPS than by SEB. SEB-based clustering, identified 3 severity-based groups of septic patients significantly different regarding mHLA-DR expression and TNFα level post-LPS, as well as 28-day mortality, and nosocomial infections. Combining the results from two independent cohorts gathering 20 HV and 60 patients, 1 cluster grouped all HV with 12% of patients. The second cluster grouped 42% of patients and contained all non-survivors. The third cluster grouped 46% of patients, including 78% of those with nosocomial infections. The molecular features of these clusters indicated a distinctive contribution of previously described genes defining a “healthy-immune response” and a “sepsis-related host response”. The third cluster was characterised by potential immune recovery that underlines the possible added value of SEB-based IFA to capture the sepsis immune response and contribute to personalised management.
An association study of C9orf3, a novel component of the renin-angiotensin system, and hypertension in diabetes
Authors: Ichikawa Mai , Konoshita Tadashi , Makino Yasukazu , Suzuki Jinya , Ishizuka Tamotsu , Nakamura Hiroyuki 
Published: September 2020

AbstractThe renin-angiotensin system (RAS) is important in the onset and course of cardiovascular, kidney, and metabolic disorders. Previous reports showed that the RAS blockade protects organs and suppress the development of type 2 diabetes mellitus. A novel component of the RAS, namely, chromosome 9 open reading frame 3 (C9orf3), was recently identified, however, its effects are unclear. We evaluated whether the genetic variant of C9orf3 is associated with morbidity of hypertension among subjects with type 2 diabetes. We enrolled 382 subjects with type 2 diabetes, 222 of whom were diagnosed with hypertension. Human leukocyte genomic DNA was isolated and a genetic variant was analyzed for a C/T variant of C9orf3 (rs4385527) via PCR analysis. The relationship between the genotype and hypertension morbidity among subjects with diabetes was examined. The proportion of the respective C9orf3 genetic variants were as follows 247 CC, 119 CT, and 16 TT. The risk of hypertension was determined to be 1.58, with a 95% confidence interval of 1.11–2.27. Moreover, the p value was 0.012 for allelic comparison and for Armitage’s trend test, with the C allele identified as the risk factor. Consequently, hypertension was markedly associated with type 2 diabetes in subjects with the C9orf3 variant, exhibiting a nearly 1.6-fold increased risk. The C variant of a new component of the RAS, C9orf3 (rs4385527) might have a considerable impact on the pathogenesis of hypertension in diabetes.
Aenigmachannidae, a new family of snakehead fishes (Teleostei: Channoidei) from subterranean waters of South India
Authors: Britz Ralf , Dahanukar Neelesh , Anoop V. K. , Philip Siby , Clark Brett , Raghavan Rajeev , Rüber Lukas 
Published: September 2020

AbstractPronounced organism-wide morphological stasis in evolution has resulted in taxa with unusually high numbers of primitive characters. These ‘living fossils’ hold a prominent role for our understanding of the diversification of the group in question. Here we provide the first detailed osteological analysis ofAenigmachanna gollumbased on high-resolution nano-CT scans and one cleared and stained specimen of this recently described snakehead fish from subterranean waters of Kerala in South India. In addition to a number of derived and unique features,Aenigmachannahas several characters that exhibit putatively primitive conditions not encountered in the family Channidae. Our morphological analysis provides evidence for the phylogenetic position ofAenigmachannaas the sister group to Channidae. Molecular analyses further emphasize the uniqueness ofAenigmachannaand indicate that it is a separate lineage of snakeheads, estimated to have split from its sister group at least 34 or 109 million years ago depending on the fossil calibration employed. This may indicate thatAenigmachannais a Gondwanan lineage, which has survived break-up of the supercontinent, with India separating from Africa at around 120 mya. The surprising morphological disparity ofAenigmachannafrom members of the Channidae lead us to erect a new family of snakehead fishes, Aenigmachannidae, sister group to Channidae, to accommodate these unique snakehead fishes.
NOTCH1 is critical for fibroblast-mediated induction of cardiomyocyte specialization into ventricular conduction system-like cells in vitro
Authors: Ribeiro da Silva Agatha , Neri Elida A. , Turaça Lauro Thiago , Dariolli Rafael , Fonseca-Alaniz Miriam H. , Santos-Miranda Artur , Roman-Campos Danilo , Venturini Gabriela , Krieger Jose E. 
Published: September 2020

AbstractCardiac fibroblasts are present throughout the myocardium and are enriched in the microenvironment surrounding the ventricular conduction system (VCS). Several forms of arrhythmias are linked to VCS abnormalities, but it is still unclear whether VCS malformations are cardiomyocyte autonomous or could be linked to crosstalk between different cell types. We reasoned that fibroblasts influence cardiomyocyte specialization in VCS cells. We developed 2D and 3D culture models of neonatal rat cardiac cells to assess the influence of cardiac fibroblasts on cardiomyocytes. Cardiomyocytes adjacent to cardiac fibroblasts showed a two-fold increase in expression of VCS markers (NAV1.5 and CONTACTIN 2) and calcium transient duration, displaying a Purkinje-like profile. Fibroblast-conditioned media (fCM) was sufficient to activate VCS-related genes (Irx3, Scn5a, Connexin 40) and to induce action potential prolongation, a hallmark of Purkinge phenotype. fCM-mediated response seemed to be spatially-dependent as cardiomyocyte organoids treated with fCM had increased expression of connexin 40 and NAV1.5 primarily on its outer surface. Finally, NOTCH1 activation in both cardiomyocytes and fibroblasts was required for connexin 40 up-regulation (a proxy of VCS phenotype). Altogether, we provide evidence that cardiac fibroblasts influence cardiomyocyte specialization into VCS-like cells via NOTCH1 signaling in vitro.
Spatial–temporal dynamics and driving factor analysis of urban ecological land in Zhuhai city, China
Authors: Hu Yunfeng , Zhang Yunzhi 
Published: September 2020

AbstractEcological land is a type of land that has considerable ecological value. Understanding the evolution of urban ecological land in Zhuhai, China, holds great significance for revealing the evolution of ecological land in the Dawan District of southern China. We explored the temporal and spatial variation in urban ecological land in Zhuhai using the transformation matrix, equivalent ecological land, landscape index and ecological land center of gravity migration methods. Multivariate logistic regression was used to analyze the mechanism of ecological land change, and a transition probability map of the ecological land in the study area was drawn. The results showed the following. (1) From 1991 to 2018, the area of ecological land in Zhuhai city continuously decreased, with a reduction in area of 274.8 km2, or 32.3%. Sharp changes mainly occurred from 1991 to 2000. (2) The ecological land in the study area has gradually become fragmented, and the degree of landscape heterogeneity has increased. Affected by the expansion of the outer edge of the city to the southwest and the construction of ecological land within the city, the center of gravity of the ecological land has shifted to the northeast by 1346 m. (3) The elevation, slope, distance from built-up land and growth rate of built-up land are important factors influencing the transformation of ecological land. In the future, rivers and shallow coastal waters, tidal flats, and grasslands in the study area have the highest probability of transformation. The Jinwan District and Xiangzhou District will face severe ecological land protection pressure. The method of spatial–temporal analysis of urban ecological land developed in this paper can be applied in similar studies on other cities, and the results obtained for Zhuhai, China, have reference value for future urban planning and ecological protection work.
The shaping of immunological responses through natural selection after the Roma Diaspora
Authors: Dobon Begoña , ter Horst Rob , Laayouni Hafid , Mondal Mayukh , Bianco Erica , Comas David , Ioana Mihai , Bosch Elena , Bertranpetit Jaume , Netea Mihai G. 
Published: September 2020

AbstractThe Roma people are the largest transnational ethnic minority in Europe and can be considered the last human migration of South Asian origin into the continent. They left Northwest India approximately 1,000 years ago, reaching the Balkan Peninsula around the twelfth century and Romania in the fourteenth century. Here, we analyze whole-genome sequencing data of 40 Roma and 40 non-Roma individuals from Romania. We performed a genome-wide scan of selection comparing Roma, their local host population, and a Northwestern Indian population, to identify the selective pressures faced by the Roma mainly after they settled in Europe. We identify under recent selection several pathways implicated in immune responses, among them cellular metabolism pathways known to be rewired after immune stimulation. We validated the interaction between PIK3-mTOR-HIF-1α and cytokine response influenced by bacterial and fungal infections. Our results point to a significant role of these pathways for host defense against the most prevalent pathogens in Europe during the last millennium.
Evaluation of low-intensity pulsed ultrasound on doxorubicin delivery in 2D and 3D cancer cell cultures
Authors: Paškevičiūtė Miglė , Januškevičienė Indrė , Sakalauskienė Kristina , Raišutis Renaldas , Petrikaitė Vilma 
Published: September 2020

AbstractThe aim of our study was to evaluate the influence of low-intensity pulsed US on the delivery of doxorubicin (DOX) into MDA-MB-231 triple-negative breast cancer and A549 non-small cell lung cancer cell 2D and 3D cultures. US with pulse repetition frequency of 10 Hz and 1 MHz center frequency was generated with peak negative pressure of 0.5 MPa and 50% duty cycle. SonoVue microbubbles were used. Spheroids were formed using 3D Bioprinting method. DOX delivery in 2D and 3D cultures was assessed using fluorescence microscopy. US without the addition of microbubbles did not enhance the penetration of DOX into monolayer-cultured cells and tumor spheroids. In the presence of microbubbles US improved the delivery of DOX into the edge end middle zones of A549 and MDA-MB-231 spheroids. Application of low-intensity pulsed US in combination with microbubbles may be a promising approach to enhance the delivery of DOX into tumor spheroids.
Prevalence, risk factors, and optimized management of moderate-to-severe thirst in the post-anesthesia care unit
Authors: Lee Chia-Wei , Liu Shih-Ting , Cheng Ya-Jung , Chiu Ching-Tang , Hsu Yu-Fen , Chao Anne 
Published: September 2020

AbstractPost-operative thirst is common and may cause intense patient discomfort. The aims of this retrospective study conducted in a high-volume post-anesthesia care unit (PACU) were as follows: (1) to examine the prevalence of moderate-to-severe post-operative thirst—defined as a numerical rating scale (NRS) score of 4 or higher, (2) to identify the main risk factors for moderate-to-severe post-operative thirst, and (3) to maximize the efficacy and safety of thirst management through a quality improvement program. During a 1-month quality improvement program conducted in August 2018, a total of 1211 adult patients admitted to our PACU were examined. Moderate-to-severe thirst was identified in 675 cases (55.8%). The use of glycopyrrolate during anesthesia was associated with moderate-to-severe thirst (71.7% versus 66.4%, respectively, p = 0.047; adjusted odds ratio: 1.46, p = 0.013). Following a safety assessment, ice cubes, room temperature water, or an oral moisturizer were offered to patients. A generalized estimating equation model revealed that ice cubes were the most effective means for thirst management—resulting in an estimated thirst intensity reduction of 0.93 NRS points at each 15-min interval assessment (p < 0.001)—followed by room temperature water (− 0.92/time-point, p < 0.001) and the oral moisturizer (− 0.60/time-point; p < 0.001). Patient satisfaction (rated from 1 [definitely dissatisfied] to 5 [very satisfied]) followed a similar pattern (ice cubes: 4.22 ± 0.58; room temperature water: 4.08 ± 0.55; oral moisturizer: 3.90 ± 0.55, p < 0.001). The use of glycopyrrolate—an anticholinergic agent that reduces salivary secretion—was the main independent risk factor for moderate-to-severe post-operative thirst. Our findings may provide clues towards an optimized management of thirst in the immediate post-operative period.
Different organic manure sources and NPK fertilizer on soil chemical properties, growth, yield and quality of okra
Authors: Adekiya Aruna Olasekan , Ejue Wutem Sunny , Olayanju Adeniyi , Dunsin Oluwagbenga , Aboyeji Christopher Muyiwa , Aremu Charity , Adegbite Kehinde , Akinpelu Olanike 
Published: September 2020

AbstractUse of organic manures to meet the nutrient requirement of crop would be an inevitable practice in the years to come for sustainable agriculture since organic manures generally improve the soil physical, chemical and biological properties. Hence, field experiments were carried out in 2017 and 2018 to compare the impact of different organic manures and NPK fertilizer on soil properties, growth, yield, proximate and mineral contents of okra (Abelmoschus esculentus L.). The treatments each year 2017 and 2018 consisted of: rabbit manure, cow dung, poultry manure, green manure [Mexican sunflower (Tithonia diversifolia Asteraceae)], pig manure, NPK 15-15-15 fertilizer applied at 120 kg N ha−1 and a control (no manure/inorganic fertilizer). The seven treatments were laid out in a randomized complete block design with three replication. Organic manures and NPK fertilizer increased the soil organic matter (OM), N, P, K, Ca and Mg (NPK fertilizer did not increase OM, Ca and Mg significantly), growth, yield, minerals, protein, ash, carbohydrate and mucilage contents of okra fruit as compared with control. Organic manures improved okra yield compared with NPK fertilizer. Okra growth and yield parameters were significantly higher in 2018 compared with 2017. Control, rabbit manure, cow dung, poultry manure, green manure, pig manure and NPK fertilizer in 2018 increased the pod yield of okra by 9.7%, 35.3%, 57.9%, 36.2%, 39.2%, 45.5% and 3.2%, respectively compare with the same treatment in 2017. Amongst various organic manures, poultry manure produced significantly higher plant growth, yield, mineral and proximate composition of okra because of its high soil chemical properties which could be related to its lowest C: N ratio, lignin and lignin: N ratio. Results also showed that okra grown during high intensity rainfall has higher yield but with reduced quality except its mucilage content. Therefore, planting of okra with poultry manure under moderate rainfall will enhance the health benefit from the fruit, however, those that desire its mucilage content planting during high rainfall is recommended.
Multimodal combination of GC × GC-HRTOFMS and SIFT-MS for asthma phenotyping using exhaled breath
Authors: Stefanuto Pierre-Hugues , Zanella Delphine , Vercammen Joeri , Henket Monique , Schleich Florence , Louis Renaud , Focant Jean-François 
Published: September 2020

AbstractChronic inflammatory lung diseases impact more than 300 million of people worldwide. Because they are not curable, these diseases have a high impact on both the quality of life of patients and the healthcare budget. The stability of patient condition relies mostly on constant treatment adaptation and lung function monitoring. However, due to the variety of inflammation phenotypes, almost one third of the patients receive an ineffective treatment. To improve phenotyping, we evaluated the complementarity of two techniques for exhaled breath analysis: full resolving comprehensive two-dimensional gas chromatography coupled to high-resolution time-of-flight mass spectrometry (GC × GC-HRTOFMS) and rapid screening selected ion flow tube MS (SIFT-MS). GC × GC-HRTOFMS has a high resolving power and offers a full overview of sample composition, providing deep insights on the ongoing biology. SIFT-MS is usually used for targeted analyses, allowing rapid classification of samples in defined groups. In this study, we used SIFT-MS in a possible untargeted full-scan mode, where it provides pattern-based classification capacity. We analyzed the exhaled breath of 50 asthmatic patients. Both techniques provided good classification accuracy (around 75%), similar to the efficiency of other clinical tools routinely used for asthma phenotyping. Moreover, our study provides useful information regarding the complementarity of the two techniques.
Genetic basis of falling risk susceptibility in the UK Biobank Study
Authors: Trajanoska Katerina , Seppala Lotta J. , Medina-Gomez Carolina , Hsu Yi-Hsiang , Zhou Sirui , van Schoor Natasja M. , de Groot Lisette C. P. G. M. , Karasik David , Richards J. Brent , Kiel Douglas P. , Uitterlinden Andre G. , Perry John R. B. , van der Velde Nathalie , Day Felix R. , Rivadeneira Fernando 
Published: September 2020

AbstractBoth extrinsic and intrinsic factors predispose older people to fall. We performed a genome-wide association analysis to investigate how much of an individual’s fall susceptibility can be attributed to genetics in 89,076 cases and 362,103 controls from the UK Biobank Study. The analysis revealed a small, but significant SNP-based heritability (2.7%) and identified three novel fall-associated loci (Pcombined ≤ 5 × 10−8). Polygenic risk scores in two independent settings showed patterns of polygenic inheritance. Risk of falling had positive genetic correlations with fractures, identifying for the first time a pathway independent of bone mineral density. There were also positive genetic correlations with insomnia, neuroticism, depressive symptoms, and different medications. Negative genetic correlations were identified with muscle strength, intelligence and subjective well-being. Brain, and in particular cerebellum tissue, showed the highest gene expression enrichment for fall-associated variants. Overall, despite the highly heterogenic nature underlying fall risk, a proportion of the susceptibility can be attributed to genetics.
Development of whole-cell and cell-free biosensors for the detection and differentiation of organic and inorganic forms of copper
Authors: Costello Alan , Parker Joanne , Clynes Martin , Murphy Richard 
Published: September 2020

Abstract
The modern world has seen exposure of bacterial communities to toxic metals at selective levels. This manifests itself both intentionally, through medicines and un-intentionally through waste streams. There is growing concern that selective exposure to metals may be linked to microbial resistance to antibiotics. For a microbe to become resistant to a specific metal it must first come in contact with it. The transition metal copper has the ability to enter bacterial cells without need for a copper specific uptake mechanism. Copper is commonly used as an antimicrobial in the healthcare industry, consumer products and as a growth promoter of livestock in the agricultural sector. Here we report a study into the uptake of different organic and inorganic sources of copper. A whole-cell bacterial biosensor was developed to quantify the specific uptake of copper from various sources. Furthermore, a cell-free sensor was utilized to investigate the response to copper sources when uptake is eliminated as a factor. The data within suggest inorganic copper to have greatly reduced uptake compared to organic sources and that there is significant difference between copper oxides, Cu2O and CuO.