bluuebunny/crossref_metadata_2025_split huggingface dataset:
Irrigation of biomass plantations may globally increase water stress more than climate change
Authors: Stenzel Fabian , Greve Peter , Lucht Wolfgang , Tramberend Sylvia , Wada Yoshihide , Gerten Dieter 
Published: September 2020

Abstract
Bioenergy with carbon capture and storage (BECCS) is considered an important negative emissions (NEs) technology, but might involve substantial irrigation on biomass plantations. Potential water stress resulting from the additional withdrawals for irrigation warrants evaluation against the avoided climate change impact. Here we quantitatively assess potential side effects of BECCS with respect to water stress by disentangling the associated drivers (irrigated bioenergy, climate, land use patterns) using comprehensive global model simulations. By considering a widespread use of irrigated BECCS to limit global warming to 1.5°C, our results suggest that both the global area and population living under severe water stress will double by the end of the 21st century, which could even exceed the impact of climate change avoided by the NEs (3°C warming). Such side-effects of achieving substantial NEs would come as an extra pressure in an already water-stressed world and could only be avoided if sustainable water management would be implemented
globally.
Evolution of codon usage in 2019-new coronavirus causing human infection
Authors: Yao Xiaoting , Xie Yanfei , Guan Xiong , Ni Silu , Zuo Chenxiang , Rahman Siddiq Ur , Chen Dekun , Ma Wentao 
Published: September 2020

Abstract
Background: The recent emergence of viral pneumonia was caused by the novel coronavirus (SARS-Cov-2), which has spread to many countries and threated the global public health.Results: In the current study, we provide a primary evolutionary analysis based on the codon usage patterns of SARS-Cov-2 genome sequences. We employed bioinformatics technologies to measure the nucleotide compositions, the relative synonymous codon usage (RSCU), the codon adaptation index (CAI), and other indices. Our results reflected that there were some similarities of codon usage bias between SARS-Cov-2 and its natural reservoirs, suggesting that SARS-Cov-2 was tended to evolve its codon usage which was comparable to that of its hosts. Additionally, various degree of adaptation to the SARS-Cov-2 host and vector were estimated, even different genes have different codon adaptation to their reservoirs. We further suggested that SARS-Cov-2 isolates were evolving at a rapid substitution rate under their translation selection pressure of their hosts.Conclusions: The findings of the present study will provide help for the understanding of the elements leading to viral evolution and adaptation to reservoirs.
Sensory Processing in Adult ADHD – A Systematic Review
Authors: Schulze Marcel , Lux Silke , Philipsen Alexandra 
Published: September 2020

Abstract
BackgroundThe way we perceive our environment is driven by our sensory nervous system and our attentional resources. Attention-deficit/hyperactivity disorder (ADHD) is a neurodevelopmental disorder characterized by inattention, impulsivity, and hyperactivity. While cognitive and behavior dysfunctions have broadly been investigated, sensory processing has received less scientific attention. It has been shown, that children with ADHD show processing and modulatory deficits in multiple sensory domains, but very few studies examine to what extent these deficits persist in adult life. We conducted a systematic review of studies investigating sensory processing in adult ADHD.Main BodyUsing the keywords ‘ADHD’ and ‘sensory processing’, Web of Science and MEDLINE database were systematically searched for all articles published up to March 2020. 53 studies were included. Mostly, visual and auditory processing are studied, few investigated multisensory audiovisual and somatosensory processing. In summary, adult ADHD is marked by increased sensory gaining and deficient sensory inhibition. These disturbed gaining and inhibitory mechanisms were most prominent in the auditory modality but also visual modality impairment in terms of stimuli modulation were evident. Electrophysiological studies show alterations across all event-related potential (ERP) components associated with distractibility at early components (bottom-up) and inhibition and stimulus discrimination at later components (top-down). Brain imaging studies on sensory processing in ADHD are scarce, few pointing to higher resting state functional connectivity in visual areas and visual crossmodal activation for auditory stimuli. ConclusionSensory processing deficits extent from childhood to adult ADHD. These deficits are mainly driven by higher distractibility by irrelevant stimuli and modulatory impairment for relevant stimuli. In future studies, the relation of impaired bottom-up and top-down attentional mechanisms should be investigated and how they contribute to sensory processing deficits and clinical symptomatology in adult ADHD. This could help to gather more information about the underling processing deficits, so that specific adjusted training can be provided, that helps to overcome deficits in daily life functioning in e.g., not producing appropriate adaptive responses in social settings.Trial registrationN/A
Efficient production of human growth factors in Escherichia coli by fusing with small protein 6HFh8
Authors: Kim Young Su , Lee Hye-Jeong , Han Man-Ho , Yoon Nam-Kyung , Kim Yeu-Chun , Ahn Jung-Oh 
Published: September 2020

Abstract
Background: Growth factors (GFs) are signaling proteins that affect cellular processes such as growth, proliferation, and differentiation. GFs are used as cosmeceuticals, exerting anti-wrinkle, anti-aging, and whitening effects, and also as pharmaceuticals to treat wounds, growth failure, and oral mucositis. However, in mammalian and bacterial cells, low productivity and expression in inclusion bodies, respectively, of GFs does not satisfy the consumer demand. Here, we aimed to develop a bacterial expression system that produces high yields of soluble GFs that can be purified in their native forms.Results: We present Fh8, an 8-kDa peptide from Fasciola hepatica with an N-terminal hexa-histidine (6HFh8), as a fusion partner for enhanced human GF production in recombinant Escherichia coli. The fusion partner harboring a tobacco etch virus (TEV) protease cleavage site was fused to the N-terminus of 10 human GFs: acidic and basic fibroblast growth factors (aFGF and bFGF, respectively), epidermal growth factor (EGF), human growth hormone (hGH), insulin-like growth factor 1 (IGF-1), vascular endothelial growth factor 165 (VEGF165), keratinocyte growth factor 1 (KGF-1), placental growth factor (PGF), stem cell factor (SCF), and tissue inhibitor of metalloproteinase 1 (TIMP-1). The fusion proteins were expressed in E. coli under the control of T7 promoter at three temperatures (25 °C, 30 °C, and 37 °C). All individual fusion proteins, except for SCF and TIMP-1, were successfully overexpressed in cytoplasmic soluble form at more than one temperature. Further, the original aFGF, IGF-1, EGF, and VEGF165 proteins were cleaved from the fusion partner by TEV protease. Five-liter fed-batch fermentation approaches for the 6HFh8-aFGF (lacking disulfide bonds) and 6HFh8-VEGF165 (a cysteine-rich protein) were devised to obtain the target protein on a g/l scale. The two GFs were successfully highly purified (>99% purity). Furthermore, they exerted similar cell proliferative effects as those of their commercial equivalents.Conclusions: We demonstrated that 6HFh8-GF fusion proteins could be overexpressed on a g/l scale in the cytoplasm of E. coli, with the GFs subsequently highly purified and maintaining their biological activity. Hence, the small protein 6HFh8 can be used for efficient mass-production of various GFs.
Context-dependent cross-modal interaction in the medial prefrontal cortex of rats
Authors: Zheng Mengyao , Xu Jinghong , Keniston Les , Wu Jing , Chang Song , Yu Liping 
Published: September 2020

Abstract
Cross-modal interaction (CMI) could significantly influence the perceptional or decision-making process in many circumstances. However, it remains poorly understood what integrative strategies are employed by the brain to deal with different task contexts. To explore it, we examined neural activities of the medial prefrontal cortex (mPFC) of rats performing cue-guided two-alternative forced-choice tasks. In a task requiring rats to discriminate stimuli based on auditory cue, the simultaneous presentation of an uninformative visual cue substantially strengthened mPFC neurons' capability of auditory discrimination mainly through enhancing the response to the preferred cue. Doing this also increased the number of neurons revealing a cue preference. If the task was changed slightly and a visual cue, like the auditory, denoted a specific behavioral direction, mPFC neurons frequently showed a different CMI pattern with an effect of cross-modal enhancement best evoked in information-congruent multisensory trials. In a choice free task, however, the majority of neurons failed to show a cross-modal enhancement effect and cue preference. These results indicate that CMI at the neuronal level is context-dependent in a way that differs from what has been shown in previous studies.
Risk Factors for Lassa Fever Infection Among Survivours at Federal Medical Centre, Owo, Ondo State, Nigeria.
Authors: Ilesanmi Olayinka Stephen , Owoeye-Lawal Oluwatosin Temitope , Aro Adewale Johnson , Afolabi Aanuoluwapo Adeyimika , Kareem Adesola Olawumi , Abejegah Chukwuyem , Ayodeji Oladele Oluwafemi 
Published: September 2020

Abstract
BackgroundThere exists paucity of information regarding successfully managed Lassa Fever (LF) patients. This study is aimed at determining the perceived risk factors for LF infection among patients who had been managed and on follow up at Federal Medical Centre, Owo, Ondo State. MethodsA cross sectional study of 101 LF survivors who had been managed and attending follow up clinic at Federal Medical Centre, Owo was done. Data were collected using semi-structured interviewer-administered questionnaire. Respondents were assessed for symptoms and factors that may have predisposed them to LF, and data analyzed with SPSS version 23. Those who had more than three identifiable risks were categorised as high risk. Descriptive statistics was done, and association between socio-demographic characteristics and high risk were explored using chi square test. ResultsThe median age of respondents was 33 years (range 8-85 years), 61.4% were males, 92.1% have heard of LF prior to being diagnosed, 55.9% heard through the mass media. The perceived cause of Lassa fever infection among respondents included consumption of food contaminated by rats’ feces among 57(56.4%) persons, while 16 (15.8) attributed their infection to contact with LF-infected persons. Among respondents with primary education and below, 5 (29.4%) had high risk of Lassa fever infection compared to 7 (8.3%) with secondary education and above (p=0.014). ConclusionMass media and other sources of information should be well harnessed in the communication of risks and preventive practices for LF. Health education should be intensified both in hospitals and schools to capture both young and old in LF prevention activities.
Asphericity of Tumor FDG Uptake in Non-small Cell Lung Cancer: Reproducibility and Implications for Harmonization in Multicenter Studies
Authors: Rogasch Julian MM , Furth Christian , Bluemel Stephanie , Radojewski Piotr , Amthauer Holger , Hofheinz Frank 
Published: September 2020

Abstract
BackgroundAsphericity (ASP) of the primary tumor’s metabolic tumor volume (MTV) in FDG-PET/CT is independently predictive for survival in patients with non-small cell lung cancer (NSCLC). However, comparability between PET systems may be limited. Therefore, reproducibility of ASP was evaluated at varying image reconstruction and acquisition times to assess feasibility of ASP assessment in multicenter studies.MethodsRetrospective study of 50 patients with NSCLC (female, 20; median age, 69 years) undergoing pretherapeutic FDG-PET/CT (median, 3.7 MBq/kg; 180 s/bed position). Reconstruction used OSEM with TOF4/16 (iterations, 4; subsets, 16; in-plane filter, 2.0, 6.4 or 9.5 mm), TOF4/8 (4 it; 8 ss; filter, 2.0/6.0/9.5 mm), PSF+TOF2/17 (2 it; 17 ss; filter, 2.0/7.0/10.0 mm) or Bayesian penalized likelihood (Q.Clear; beta, 600/1750/4000). Resulting reconstructed spatial resolution (FWHM) was determined from hot sphere inserts of a NEMA IEC phantom. Data with approx. 5 mm FWHM were retrospectively smoothed to achieve 7 mm FWHM. List mode data were rebinned for acquisition times of 120/90/60s. Threshold-based delineation of primary tumor MTV was followed by evaluation of relative ASP/SUVmax/MTV differences between datasets and resulting proportions of discordantly classified cases.ResultsReconstructed resolution for narrow/medium/wide in-plane filter (or low/medium/high beta) was approx. 5/7/9 mm FWHM. Comparing different pairs of reconstructed resolution between TOF4/8, PSF+TOF2/17, Q.Clear and the reference algorithm TOF4/16, ASP differences were lowest at FWHM of 7 vs. 7 mm. Proportions of discordant cases (ASP >19.5% vs. ≤19.5%) were also lowest at 7 mm (TOF4/8, 2%; PSF+TOF2/17, 4%; Q.Clear, 10%). Smoothing of 5 mm data to 7 mm FWHM significantly reduced discordant cases (TOF4/8, 38% reduced to 2%; PSF+TOF2/17, 12% to 4%; Q.Clear, 10% to 6%) resulting in proportions comparable to original 7 mm data. Shorter acquisition time only increased proportions of discordant cases at <90s. ConclusionsASP differences were mainly determined by reconstructed spatial resolution, and multicenter studies should aim at comparable FWHM (e.g., 7 mm; determined by in-plane filter width). This reduces discordant cases (high vs. low ASP) to an acceptable proportion for TOF and PSF+TOF of <5% (Q.Clear: 10%). Data with better resolution (i.e. lower FWHM) could be retrospectively smoothed to the desired FWHM resulting in a comparable number of discordant cases.
Reverse Shoulder Arthroplasty Versus Antegrade Nailing for 3- and 4-Part Proximal Humeral Fractures in Elderly Patients.
Authors: AMRI Khalil , AYARI Rabie , TLEMSANI Karim LATRECH , ABDENNADHER Achraf , SALAH Mohamed BEN , DHAHRI Rim , CHOURABI Chadia , NOUISRI Lotfi 
Published: September 2020

Abstract
Introduction: Proximal humerus fractures are prevalent and widespread, accounting for 5% of all fractures, mainly in the elderly osteoporotic patients following a low-level trauma. Three-and four-part fractures represent 13 to 16% of these fractures and are generally associated with a worse prognosis. Depending on the fracture type and the patient status, several therapeutic options would be available ranging from osteosynthesis to arthroplasty. The aim of our study was to compare the functional and radiological results, as well as the complications of reverse shoulder arthroplasty and anterograde nailing when managing these fractures. Methods: A retrospective and descriptive study was conducted within the orthopedic surgery department of the Military Hospital of Instruction of Tunis, over a period of 42 months, from January 2016 to June 2019 with an average follow-up of 18 months. We included 31 cases of complex fractures of the proximal humerus in elderly patients, aged over 65 years, 16 of whom were treated by reverse total arthroplasty and 15 by antegrade nailing. Results: Our patient pool included 8 men and 23 women with a mean age of 73 years, ranging from 65 to 85. A low-energy fall was the main cause of the fractures with the dominant side being affected in 55% of cases. Four-part fractures were predominant representing 68% of the fractures. Constant and DASH scores at the last follow-up were at 68 and 24 respectively. Most patients were satisfied with the outcome of their surgeries, with an overall satisfaction rate of 87%. The complications rate was in accordance with the literature. Functional results in the group treated with reverse total shoulder arthroplasty were better with a lower complications rate. Conclusion: Reverse total shoulder arthroplasty has become an interesting surgical alternative, as the functional and anatomical results in our study were promising, while elucidating an improved quality of life for our patients. These findings have encouraged us to continue using this technique as a first-line choice for complex fractures of the proximal humerus in the elderly
Comparison of the Effects of Transcranial Direct Current Stimulation and Mindfulness-Based Stress Reduction on Mental Fatigue, Quality of Life and Aggression in Mild Traumatic Brain Injury Patients: A Randomized Clinical Trial
Authors: Shirvani Sheida , davoudi mohammadreza , Shirvani Masoud , Koleini Peiman , Panah Safora Hojat , shoshtari Fatemeh , Omidi Abdollah 
Published: September 2020

Abstract
Background: The rate of traumatic brain injuries (TBIs) due to the accidents is high around the world. Patients with mild TBIs may suffer from some psychological disorders, including aggression, and mental fatigue, and thus their quality of life decreased. Among different treatments for TBI, two treatments, namely transcranial direct current stimulation (tDCS), and mindfulness-based stress reduction (MBSR) have shown to be effective. So, this study aimed to compare the effects of these two treatments on mental fatigue, aggression and quality of life in mTBI patients.Materials and methods: This randomized controlled trial was conducted on 2000 TBI patients referred to emergency and neurosurgery departments of Shahid Beheshti Hospital, Kashan, Iran, in 2017. They were selected using the convenience sampling method. Data were collected using the mental fatigue scale, the World Health Organization Quality of Life- BREF (short version), and the Buss-Perry Aggression Questionnaires. Then, the data were analyzed using a Mixed Repeated Measures ANOVAs, and the Levene and Kolmogorov-Smirnov tests by SPSS-23 software.Results: Data were collected from 48 patients with mTBI. The mean age of patients in the three groups of MBSR, tDCS and control were 69.38 + 6.11 (25% male), 25.40 + 12.11 (25% male) and 69.37 + 0.2 (18.8% male), respectively. There was no significant difference between the three groups in terms of mental fatigue, quality of life and aggression (P <0.05). Also, the results showed that there was a significant difference between the main effect of time and the interaction between time and group (P <0.001).Conclusions: Both MBSR and tDCS methods are effective in reducing the mental fatigue and aggression and increasing quality of life of mTBI patients; MBSR treatment, as indicated in the present study, can be more effective than tDCS in patients with mTBI.Trial registration: Thailand Registry of Clinical Trials, TCTR20180827003 Registered on August 24, 2018.
Enlightening the Taxonomy Darkness of Human Gut Microbiomes With Cultured Biobank
Authors: Liu Chang , Du Meng-Xuan , Abuduaini Rexiding , Yu Hai-Ying , Li Dan-Hua , Wang Yu-Jing , Zhou Nan , Jiang Min-Zhi , Niu Peng-Xia , Han Shan-Shan , Chen Hong-He , Shi Wen-Yu , Wu Linhuan , Xin Yu-Hua , Ma Juncai , Zhou Yuguang , Jiang Cheng-Ying , Liu Hong-Wei , Liu Shuang-Jiang 
Published: September 2020

Abstract
Background The cultivated gut microbial resource plays essential role in gut microbiome studies such as gut microbial function and their interactions with host. Though several major studies had been performed to understand the cultured human gut microbiota, up to 70% of the Unified Human Gastrointestinal Genome species remain uncultivated and their taxonomy is not clear. Large-scale gut microbial isolation and identification and their access to pubic are imperative for gut microbial studies and for understanding of the human gut microbial functions.Results Here, we report the construction of an human Gut Microbial Biobank (hGMB) (homepage: hgmb.nmdc.cn) by large-scale cultivation of 10,558 isolates from 239 feces of healthy Chinese volunteers, and deposited 1,170 strains representing 404 different species in International Depository Authority for long-term preservation and public access worldwidely. We discovered and denominated 107 new species, and proposed 28 new genera and 3 new families. The new species and their newly sequenced genomes uncovered 16 “most-wanted” or “medium priority” taxa proposed by the Human Microbiome Project and 42 previously-uncultured MAGs in IGGdb, respectively. The hGMB represented over 80% of the common and dominant human gut microbial genera or species of global human gut 16S rRNA gene amplicon data (n=11,647), and covered 70% of the known genes (KEGG Orthologys) and 10% of the functionally-unknown genes in the global human gut gene catalogs. Conclusions A publically accessible human Gut Microbial Biobank (hGMB) that contains 1,170 strains and represents 404 human gut microbial speces is estabolished. The hGMB expands the currently known, taxonomically-characterized gut microbial resources and genomic repository by adding 107 new species and 115 new genomes of human gut microbes. Based on the newly discovered species in this study, 28 new genera and 3 new families of human gut microbes were identified and proposed.
Studies on washability characteristics of low-volatile Indian coking coal by differential crushing
Authors: Chandra Sugali Sekhar 
Published: September 2020

Abstract
The effect of differential crushing on liberation characteristics has been studied for a low volatile coking coal of Indian origin through washability studies. Two parameters, namely “Index of Washability” (IW) and “Near Gravity Material Index” (NGMI), are used to describe the ease of washability. The ROM Coal is crushed to four different top sizes namely, 75 mm, 25 mm, 13 mm and 6 mm.On the basis of calculated IW it is observed that relative ease of washabiltiy increases with decrease in top size. From calculated IW values it may be said that this coal can be economically beneficiated using gravity process after crushing to -6 mm size. From the calculated NGMI values, the critical specific gravities have been estimated and the values for crushing to -75 mm,-25 mm,-13 mm and − 6 mm are 1.65, 1.68, 1.53 and 1.58 respectively. These critical specific gravity values suggest the separation at this specific gravity range is most difficult task using gravity methods. From NGMI analysis, it may be said that the NGMI values for coals crushed to -25 mm & -6 mm are identical (≈ 0.18) at 17% clean coal ash content. This suggests that with similar degree of difficulty, clean coal of 17% ash can be produced from these two different crushing sizes. In order to increase the yield for the clean coal of 17% ash, the decision on blending these two size coals may need to be taken.