bluuebunny/crossref_metadata_2025_split huggingface dataset:
Proteome-wide and lysine crotonylation profiling reveals the importance of crotonylation in chrysanthemum (Dendranthema grandiforum) under low-temperature
Authors: Lin Ping , Bai Hui-ru , He Ling , Huang Qiu-xiang , Zeng Qin-han , Pan Yuan-zhi , Jiang Bei-bei , Zhang Fan , Zhang Lei , Liu Qin-lin 
Published: November 2020

Abstract
Background: Low-temperature severely affects the growth and development of chrysanthemum which is one kind of ornamental plant well-known and widely used in the world. Lysine crotonylation is a recently identified post-translational modification (PTM) with multiple cellular functions. However, lysine crotonylation under low-temperature stress has not been studied.Results: Proteome-wide and lysine crotonylation of chrysanthemum at low-temperature was analyzed using TMT (Tandem Mass Tag) labeling, sensitive immuno-precipitation, and high-resolution LC-MS/MS. The results showed that 2017 crotonylation sites were identified in 1199 proteins. Treatment at 4 °C for 24 h and −4 °C for 4 h resulted in 393 upregulated proteins and 500 downregulated proteins (1.2-fold threshold and P < 0.05). Analysis of biological information showed that lysine crotonylation was involved in photosynthesis, ribosomes, and antioxidant systems. The crotonylated proteins and motifs in chrysanthemum were compared with other plants to obtain orthologous proteins and conserved motifs. To further understand how lysine crotonylation at K136 affected APX (ascorbate peroxidase), we performed a site-directed mutation at K136 in APX. Site-directed crotonylation showed that lysine decrotonylation at K136 reduced APX activity, and lysine complete crotonylation at K136 increased APX activity.Conclusion: In summary, our study comparatively analyzed proteome-wide and crotonylation in chrysanthemum under low-temperature stress and provided insights into the mechanisms of crotonylation in positively regulated APX activity to reduce the oxidative damage caused by low-temperature stress. These data provided an important basis for studying crotonylation to regulate antioxidant enzyme activity in response to low-temperature stress and a new research ideas for chilling-tolerance and freezing-tolerance chrysanthemum molecular breeding.
Interaction study of Pasteurella multocida with culturable aerobic bacteria isolated from porcine respiratory tracts using coculture in conditioned media
Authors: Hanchanachai Nonzee , Chumnanpuen Pramote , E-KOBON Teerasak 
Published: November 2020

Abstract
Background: The porcine respiratory tract harbours multiple microorganisms, and the interactions between these organisms could be associated with animal health status. Pasteurella multocida is a culturable facultative anaerobic bacterium isolated from healthy and diseased porcine respiratory tracts. The interaction between P. multocida and other aerobic commensal bacteria in the porcine respiratory tract is not well understood. This study aimed to determine the interactions between porcine P. multocida capsular serotype A and D strains and other culturable aerobic bacteria isolated from porcine respiratory tracts using a coculture assay in conditioned media followed by calculation of the growth rates and interaction parameters.Results: One hundred and sixteen bacterial samples were isolated from five porcine respiratory tracts, and 93 isolates were identified and phylogenetically classified into fourteen genera based on 16S rRNA sequences. Thirteen isolates from Gram-negative bacterial genera and two isolates from the Gram-positive bacterial genus were selected for coculture with P. multocida. From 17x17 (289) interaction pairs, the majority of 220 pairs had negative interactions indicating competition for nutrients and space, while 17 pairs were identified as mild cooperative or positive interactions indicating their coexistence. All conditioned media, except those of Acinetobacter, could inhibit P. multocida growth. Conversely, the conditioned media of P. multocida also inhibited the growth of nine isolates plus themselves. Conclusion: Negative interaction was the major interactions among the coculture of these 15 representative isolates and the coculture with P. multocida. The conditioned media in this study might be further analysed to identify critical molecules and examined by the in vivo experiments. The study proposed the possibility of using these molecules in conditioned media to control P. multocida growth.
A HAWAII-2RG infrared camera operated under fast readout mode for solar polarimetry
Authors: Hanaoka Yoichiro , Katsukawa Yukio , Morita Satoshi , Kamata Yukiko , Ishizuka Noriyoshi 
Published: November 2020

Abstract
Polarimetry is a crucial method to investigate solar magnetic fields. From the viewpoint of space weather, the magnetic field in solar laments, which occasionally erupt and develop into interplanetary flux ropes, is of particular interest. To measure the magnetic field in laments, high-performance polarimetry in the near-infrared wavelengths employing a high-speed, large-format detector is required; however, so far, this has been difficult to be realized. Thus, the development of a new infrared camera for advanced solar polarimetry has been started, employing a HAWAII-2RG (H2RG) array by Teledyne, which has 2048 x 2048 pixels, focusing on the wavelengths in the range of 1.0{1.6 um. We solved the problem of the difficult operation of the H2RGs under "fast readout mode" synchronizing with high-speed polarization modulation by introducing a "MACIE" (Markury ASIC Control and Interface Electronics) interface card and new assembly codes provided by Markury Scientific This enables polarization measurements with high frame-rates, such as 29{117 frames per seconds, using a H2RG. We conducted experimental observations of the Sun and confirmed the high polarimetric performance of the camera.
Measuring capabilities in health and physical activity promotion: a systematic review.
Authors: Till Maike , Abu-Omar Karim , Ferschl Susanne , Reimers Anne Kerstin , Gelius Peter 
Published: November 2020

Abstract
Background: The capability approach by Amartya Sen and Martha Nussbaum has gained increasing attention in the field of public health. As it combines individual, social and structural factors and shifts the focus of attention from the actual behavior towards available options for health behaviors that people can actually choose from, it may help advance our understanding of complex health issues. Objectives: The aim of this article is to identify and describe tools available to measure capabilities within the context of health, with a specific focus on capabilities for health-enhancing physical activity.Method: We conducted a systematic literature review using 11 databases covering scientific journal articles published in English or German between the years 2000 and 2020 with a focus on capabilities for health or physical activity. Results: We found a total of 51 articles meeting our inclusion criteria. Four articles measured capabilities using qualitative methods, one combined qualitative and quantitative methods, while the rest used quantitative methods. We identified a total 11 different capability questionnaires, all showing moderate to good validity/reliability. Only one questionnaire and one interview-based tool specifically dealt with capabilities for health enhancing physical activity.Conclusion: Although we were able to identify measurement tools for capabilities in health, this review has shown that there is no generic tool available for the measurement across all population- and age-groups, and tools focusing on physical activity are scarce. However, our results can be used as guide for future projects that aim at measuring capabilities.
Factors Influencing the Deterioration from Cognitive Decline of Normal Aging to Dementia among Nursing Home Residents
Authors: Hayajneh Audai A. , Rababa Mohammad , Alghwiri Alia A. , Masha'al Dina 
Published: November 2020

Abstract
Background: A dearth of differential research exists regarding the determinants of mild cognitive impairment (MCI) and moderate cognitive impairment or dementia among nursing home residents. This study aimed to identify and examine the association between medical factors (number of comorbidities, hospitalization, disability, depression, frailty and quality of life) and moderate cognitive impairment or dementia in nursing homes residents.Methods: A cross-sectional design was used in this study. Convenience sampling of 182 participants was conducted in nursing homes located in the central part of Jordan. Montreal cognitive assessment (MoCA) was used to screen both MCI and moderate cognitive impairment or dementia. Bivariate analysis, including t-test and ANOVA test, and logistic and linear regression models were used to examine and identify the medical factors associated with moderate cognitive impairment or dementia compared to mild cognitive impairment.Results: Most nursing home residents had MCI (87.4%) compared to a few with moderate cognitive impairment or dementia. Age (t = -2.773), number of comorbidities (t = -4.045), depression (t = -4.809), frailty (t = -4.038), and quality of life physical (t = 3.282) and mental component summaries (t = 2.469) were significantly different between the stages of cognitive impairment. Marital status (t = -4.050, p < 0.001), higher-income (t = 3.755, p < 0.001), recent hospitalization (t = 2.622,p = 0.01), depression (t = -2.737, p = 0.007), and frailty (t = 2.852, p = 0.005) were significantly associated with mental ability scores among nursing home residents.Conclusion: The coexistence of comorbidities and depression among nursing home residents with MCI necessitates prompt management by healthcare providers to combat depressive symptoms in order to delay the dementia trajectory among at-risk residents. Trail registration: ClinicalTrials.gov NCT04589637, October 15,2020, Retrospectively registered.
The sniffing bead system, an olfactory dysfunction screening tool for geriatric subjects: A cross-sectional study
Authors: Min Hyun Jin , Kim Sun Mi , Han Doug Hyun , Kim Kyung Soo 
Published: November 2020

Abstract
Background: This study aimed to develop a simple and one-off olfactory screening test, the sniffing bead system, for general clinical use in older adults. Methods: In this cross-sectional study, geriatric subjects (aged > 50 years) who underwent neurocognitive and olfactory function tests were included. Overall, 137 subjects were enrolled, and the study was conducted at Chung-Ang University, Seoul, Korea. Olfactory function was measured by obtaining the scores of the sniffing bead system using 2-phenylethyl alcohol, n-butanol, and the YSK olfactory function test. Time taken for each olfactory function test was also measured.Results: The score of the 2-phenylethyl alcohol sniffing bead test was 2.58 ± 1.52, which was significantly associated with the YSK_threshold (2.41 ± 1.79) (p < 0.001, Pearson's correlation coefficient = 0.429), YSK_identification (8.93 ± 3.25) (p = 0.014, Pearson's correlation coefficient = 0.208) and YSK_threshold-discrimination-identification (17.46 ± 5.49) (p < 0.001, Pearson's correlation coefficient = 0.316) test scores. In the normal cognitive function group, YSK_threshold (p < 0.001, Pearson's correlation coefficient = 0.479), YSK_identification (p = 0.003, Pearson's correlation coefficient = 0.316), and YSK_threshold-discrimination-identification (p < 0.001, Pearson's correlation coefficient = 0.429) were significantly correlated with the scores of the 2-phenylethyl alcohol sniffing bead system. In the impaired cognitive function group, the YSK_threshold (p = 0.002, Pearson's correlation coefficient = 0.415) and YSK_ threshold-discrimination-identification (p = 0.004, Pearson's correlation coefficient = 0.385) were significantly correlated with the scores of the 2-phenylethyl alcohol sniffing bead system. Time taken for the 2-phenylethyl alcohol sniffing bead system was 5.00 ± 1.51 min, which was significantly lower than that for the YSK_threshold-discrimination-identification (20.43 ± 5.29 min) (p < 0.001). The scores of the 2-phenylethyl alcohol sniffing bead system were significantly correlated with those of the n-butanol sniffing bead system (3.50 ± 1.21) (p < 0.001, Pearson's correlation coefficient = 0.315). Conclusions: This sniffing bead system was specifically designed for screening olfactory function in older adults, and it may allow for the rapid and accurate assessment of olfactory dysfunction.
Health Care Staff Support for Mothers in NICU: A Focused Ethnography Study
Authors: Negarandeh Reza , Hassankhani Hadi , jabraeili mahnaz , Abbaszadeh mohammaf , Best Amy 
Published: November 2020

Abstract
Background: Mothers of premature infants in the neonatal intensive care unit (NICU) have complex needs and require a significant amount of support during the NICU admission. However, little is known about mothers support needs in the NICU. The aim of study was to explore mothers and health staff experiences and care practices meeting support needs of the mothers in the NICU. Methods: A focused ethnographic approach was adopted. Observation and interviews with 21 mothers, 18 nurses and 5 physicians were undertaken over a seven month period. Qualitative data analysis was conducted using the Roper and Shapira (2000) five-step framework. Result: Two main themes of “Insufficient attention to mothers’ support needs (Subthemes: inadequate accompany the mother in care, assigning monitoring and care to the mother, inadequate sharing of medical information) and “Supporting the mothers in certain circumstances” (Subthemes: reassuring the mother, supporting mothers with reduced functional capacity, providing information) were obtained. Conclusion: This study showed that, there is a gap exists between the level of expected support and the actual support received by mothers. In this study, Staff support for mothers was selective support that in many cases was inadequate and so support was provided only when the mother greatly needed support. However, most staff considered meeting maternal support as a necessity, they often confirmed inadequate maternal support in NICU and considered workload as a barrier to maternal support.
Transcriptome differences between Cupriavidus necator NH9 grown with 3-chlorobenzoate and that grown with benzoate
Authors: Moriuchi Ryota , Dohra Hideo , Kanesaki Yu , Ogawa Naoto 
Published: November 2020

Abstract
BackgroundAromatic compounds derived from human activities are often released into the environment. Many of them, especially halogenated aromatics, are persistent in nature and pose threats to organisms. Therefore, the microbial degradation of these compounds has been studied intensively. Our laboratory has studied the expression of genes in Cupriavidus necator NH9 involved in the degradation of 3-chlorobenzoate (3-CB), a model compound for studies on bacterial degradation of chlorinated aromatic compounds. In this study aimed at exploring how this bacterium has adapted to the utilization of chlorinated aromatic compounds, we performed RNA-seq analysis of NH9 cells cultured with 3-CB, benzoate (BA), or citric acid (CA). The purpose of these analyses was to identify differentially expressed genes encoding products with various biological functions involved in the degradation of 3-CB and BA.ResultsDifferential expression analysis confirmed strong induction of genes encoding enzymes in degradation pathways of 3-CB and BA, including benABCD (more than 256-fold compared with CA) encoding benzoate 1,2-dioxygenase involved in initial hydroxylation of both 3-CB and BA, and cbnABCD (more than 200-fold compared with BA and CA) encoding enzymes of chlorocatechol ortho-cleavage pathway. Four genes encoding major facilitator superfamily transporters were specifically induced by 3-CB or BA, and one cluster of genes encoding components of the ATP-binding cassette transporter system was significantly induced by 3-CB. Stress response genes encoding chaperones, proteases, the phosphate transporter PstBACS, and superoxide oxidase were upregulated in response to 3-CB and/or BA. Gene Ontology enrichment analysis revealed that genes encoding dioxygenases were upregulated by both 3-CB and BA. Intriguingly, the “cell motility,” “signal transduction,” and “chemotaxis” terms were significantly upregulated by BA compared with 3-CB. Consistent with this, in semi-solid agar plate assays, NH9 cells showed stronger chemotaxis to BA than to 3-CB.ConclusionsOur results showed that the chemotaxis behavior of NH9 differs between 3-CB and BA. We inferred that NH9 has not fully adapted to the utilization of chlorinated benzoate, unlike its analogous aromatic compound BA, in nature.
Mild Hyponatremia Is Associated with Low Skeletal Muscle Mass, Physical Function Impairment, and Depressive Mood in the Elderly
Authors: Fujisawa Chisato , Umegaki Hiroyuki , Sugimoto Taiki , Samizo Satoshi , Huang Chi Hsien , Fujisawa Haruki , Sugimura Yoshihisa , Kuzuya Masafumi , Toba Kenji , Sakurai Takashi 
Published: November 2020

Abstract
Background: Mild hyponatremia (serum sodium 130-135 mEq/L) is a common electrolyte disorder in the elderly. However, its association with both sarcopenia and cognitive function remains to be clarified. Therefore, here we investigated the association of mild hyponatremia with skeletal muscle mass, physical function, and cognitive function in the elderly. Methods: We enrolled 75 participants with mild hyponatremia and 2907 with normonatremia (serum sodium, 136-145 mEq/L) aged ≥ 70 years who visited the Memory Disorder Outpatient Center of Japan’s National Center for Geriatrics and Gerontology. Skeletal muscle mass index (SMI), grip strength (GS), walking speed (WS), one-leg standing (OLS) test times, and neuropsychological test scores were determined. Results: One-way analysis of covariance showed that elderly participants with mild hyponatremia had lower SMI (7.1 ± 0.2, 7.2 ± 0.2 kg/m2, p = 0.04), weaker GS (19.1 ± 1.9 vs 21.4 ± 1.8 kg, p = 0.01), slower WS (0.9 ± 0.1 vs 1.1 ± 0.1 m/s, p = 0.001), and higher GDS- 15 score (6.4 ± 0.9 vs 5.2 ± 0.9, p = 0.002) than those with normonatremia. Multiple logistic regression analysis indicated that mild hyponatremia was independently associated with sarcopenia (odds ratio [OR]: 2.2, p = 0.02), slower WS (OR: 5.3, p = 0.04) and shorter OLS time (OR: 2.5, p = 0.02) as well as with severe depressive mood (OR: 2.6 p = 0.006) but not with SMI (OR: 1.6, p = 0.2) or GS (OR: 1.9, p = 0.09). Conclusions: Our results suggest that elderly people with even mild hyponatremia had physical function impairment and depressive mood.
Characterization of florfenicol resistance genes in the coagulase-negative Staphylococcus&nbsp;(CoNS) isolates and genomic features of&nbsp;a&nbsp;multidrug-resistant Staphylococcus lentus strain&nbsp;H29
Authors: Wu Chongyang , Zhang Xueya , Liang Jialei , Li Qiaoling , Lin Hailong , Lin Chaoqing , Liu Hongmao , Zhou Danying , Lu Wei , Sun Zhewei , Lin Xi , Zhang Hailin , Li Keiwei , Xu Teng , Bao Qiyu , Lu Junwan 
Published: November 2020

Abstract
Background: With the wide use of florfenicol to prevent and treat the bacterial infection of domestic animals, the emergence of the florfenicol resistance bacteria is increasingly serious. It is very important to elucidate the molecular mechanism of the bacteria’s resistance to florfenicol.Methods: The minimum inhibitory concentration (MIC) levels were determined by the agar dilution method, and polymerase chain reaction (PCR) was conducted to analyze the distribution of florfenicol resistance genes in 39 CoNS strains isolated from poultry and livestock animals and seafood. The whole genome sequence of one multidrug resistant strain, Staphylococcus lentus H29, was characterized, and comparative genomics analysis of the resistance gene-related sequences was also performed.Results: As a result, the isolates from the animals showed a higher resistance rate (23/28, 82.1%) and much higher MIC levels to florfenicol than those from seafood. Twenty-seven animal isolates carried 37 florfenicol resistance genes (including 26 fexA, 6 cfr and 5 fexB genes) with one carrying a cfr gene, 16 each harboring a fexA gene, 5 with both a fexA and a fexB genes and the other 5 with both a fexA and a cfr genes. On the other hand, all 11 isolates from seafood were sensitive to florfenicol, and only 3 carried a fexA gene each. The whole genome sequence of S. lentus H29 was composed of a chromosome and two plasmids (pH29-46, pH29-26) and harbored 11 resistance genes, including 6 genes [cfr, fexA, ant(6)-Ia, aacA-aphD, mecA and mph(C)] encoded on the chromosome, 4 genes [cfr, fexA, aacA-aphD and tcaA] on pH29-46 and 1 gene (fosD) on pH29-26. We found that the S. lentus H29 genome carried two identical copies of the gene arrays of radC-tnpABC-hp-fexA (5,671 bp) and IS256-cfr (2,690 bp), of which one copy of the two gene arrays was encoded on plasmid pH29-46, while the other was encoded on the chromosome.Conclusions: The current study revealed the wide distribution of florfenicol resistance genes (cfr, fexA and fexB) in animal bacteria, and to the best of our knowledge, this is the first report that one S. lentus strain carried two identical copies of florfenicol resistance-related gene arrays.
Diversity and plant growth-promoting potential of (un)culturable bacteria in the Hedera helix phylloplane
Authors: Stevens Vincent , Thijs Sofie , Vangronsveld Jaco 
Published: November 2020

Abstract
BackgroundA diverse community of microbes naturally exists on the phylloplane, the surface of leaves. It is one of the most prevalent microbial habitats on earth and bacteria are the most abundant members, living in a community that is highly dynamic. While culture-independent approaches greatly increased our knowledge microbial communities such as the phylloplane, one of the challenges for microbiologists today remains to develop strategies to “culture the uncultured”.ResultsWe isolated bacteria from the phylloplane of Hedera helix (common ivy), a widespread evergreen, using the growth media LB, LB01, YMA, YFlour and YEx. We further included a comparison with the uncultured phylloplane, which contained the highest intra-sample (alpha) diversity. Inter-sample (beta) diversity shifts from LB and LB01 containing the highest amount of resources to YMA and YFlour which are more selective, and YEx which is more limited but also more varied in resources. The H. helix phylloplane is dominated by Proteobacteria, Actinobacteria, Bacteroidetes and Firmicutes in a distribution also found in other phyllosphere studies which further strengthens the finding that the phyllosphere community composition on higher taxonomic level is similar across various host plant species. All growth media more or less equally favored Actinobacteria and Gammaproteobacteria, whereas Bacteroidetes could only be found on LB01, YEx and YMA. LB and LB01 greatly favored Firmicutes and YFlour was most selective for Betaproteobacteria. At genus level, LB favored the growth of Bacillus and Stenotrophomonas, while YFlour was most selective for Burkholderia and Curtobacterium. The in vitro plant growth promotion (PGP) profile that we obtained by testing 200 isolates constitutes an important first step to find candidates with advantageous traits within microbe-assisted approaches. Our isolation effort also resulted in a significant collection of bacterial strains underrepresented in public databases, mostly from the phylum Actinobacteria.ConclusionsThis study contributes as a case study of bacterial culturability including an evaluation of five different growth media, a comparison with the uncultured H. helix phylloplane community and its relation with functional characteristics such as PGP potential which help us to understand the ecological and functional role of microbial members living in the phylloplane.