bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genome-wide identification and expression analysis of the calmodulin-binding transcription activator (CAMTA) gene family in wheat (Triticum aestivum L.)
Authors: Yang Fan , Dong Fushuang , Liu Yongwei , Chai Jianfang , Zhao He , Lv Mengyu , Zhou Shou 
Published: February 2020

Abstract
Plant calmodulin-binding transcription activator (CAMTA) proteins play important roles in hormone signal transduction, developmental regulation, and environmental stress tolerance. However, in wheat, the CAMTA gene family has not been systematically characterized. In this work, 15 wheat CAMTA genes were identified using a genome-wide search method. Their chromosome location, physicochemical properties, subcellular localization, gene structure, protein domain, and promoter cis-elements were systematically analyzed. Phylogenetic analysis classified the TaCAMTA genes into three groups (groups A, B, and C), numbered 7, 6, and 2, respectively. The results showed that most TaCAMTA genes contained stress-related cis-elements. Finally, to obtain tissue-specific and stress-responsive candidates, the expression profiles of the TaCAMTAs in various tissues and under biotic and abiotic stresses were investigated. Tissue-specific expression analysis showed that all of the TaCAMTA genes were expressed in multiple tissues, suggesting that the wheat CAMTA genes may play important roles in growth and development. Under abiotic stress, TaCAMTA1, 3, and 6 were up-regulated after drought treatment, while the expression levels of TaCAMTA1 and 3 were induced after cold treatment, indicating that they may be involved in drought and cold stress response. This work systematically identified the CAMTA gene family in wheat at the whole-genome-wide level, providing important candidates for further functional analysis in developmental regulation and the stress response in wheat.
Wee1 Inhibition Enhances the Anti-Tumor Effects of Capecitabine in Preclinical Models of Triple-Negative Breast Cancer.
Authors: Pitts Todd , Simmons Dennis M , Bagby Stacey M , Hartman Sarah J , Yacob Betelehem W , Gittleman Brian , Tentler John J , Cittely Diana , Ormond D Ryan , Messersmith Wells A , Eckhardt S Gail , Diamond Jennifer R 
Published: February 2020

Abstract

Background: Triple-negative breast cancer (TNBC) is an aggressive subtype defined by lack of hormone receptor expression and non-amplified HER2. Adavosertib (AZD1775) is a potent, small molecule, ATP-competitive inhibitor of the Wee1 kinase that potentiates the activity of many DNA-damaging chemotherapeutics and is currently in clinical development for multiple indications. The purpose of this study was to investigate the combination of AZD1775 and capecitabine/5-FU in preclinical TNBC models. Methods: TNBC cell lines were treated with AZD1775 and 5-FU and cellular proliferation was assessed in real-time using IncuCyte® Live Cell Analysis. Apoptosis was assessed via the Caspase-Glo 3/7 assay system. Western blotting was used to assess changes in expression of downstream effectors. TNBC PDX models were treated with AZD1775, capecitabine, or the combination and assessed for tumor growth inhibition. Results: From the initial PDX screen, two of the four TNBC PDX models demonstrated a better response in the combination treatment than either of the single agents. As confirmation, two PDX models were expanded for statistical comparison . Both PDX models demonstrated a significant growth inhibition in the combination versus either of the single agents. (TNBC012, p<0.05 combo vs adavosertib or capecitabine, TNBC013, p<0.01 combo vs adavosertib or capecitabine ). An enhanced antiproliferative effect was observed in the adavosertib/5-FU combination treatment as measured by live cell analysis. An increase in apoptosis was observed in two of the four cell lines in the combination when compared to single agent treatment. Treatment with single agent adavosertib resulted in an increase in p-CDC2 in a dose dependent manner that was also observed in the combination treatment. Similar results were observed with γH2AX in two of the four cell lines tested. No significant changes were observed in Bcl-xL following treatment in any of the cell lines. Conclusions: The combination of adavosertib and capecitabine/5-FU demonstrated enhanced combination effects both in vitro and in vivo in preclinical models of TNBC. These results support the clinical investigation of this combination in patients with TNBC, including those with brain metastasis given the CNS penetration of both agents.
Knockdown lncRNA Neat1 regulates the activation of microglia and alleviate neuronal apoptosis through AKT/STAT3 pathway after cerebral ischemic reperfusion.
Authors: Ni Xunran , Su Qian , Xia Wenbo , Zhang Yanli , Jia Kejuan , Li Guozhong , Su Zhiqiang 
Published: February 2020

Abstract
Background Acute cerebral ischemia may cause serious consequences, one of them is brain injury caused by the uncontrolled reperfusion which occurs once the circulation is re-established. Neat1 is one of long non-coding RNA presents important roles in the immune system. However, the potential roles and underlying molecular mechanisms of it in cerebral ischemic reperfusion injury is still unclear.The aim of the present study was to investigate the function of LncRNA Neat1 in cerebral ischemic reperfusion injury and its possible advantage for neure.MethodsIn our study, oxygen glucose deprivation was used in vitro to mimic cerebral I/R injury. CCK8 was used to mesure cell viability and flow cytometry measured cell apoptosis. qRT-pcr was used to measure the phenotypic marker of M1 and M2 microglia, western blot was used to detect the protein expression of AKT/STAT3 pathway.ResultsKnockdown the LncRNA Neat1 alleviated the apoptosis induced by OGD/R and increased the cell viability.We further provided that Neat1 may inhibite microglia polarize to M1 phenotype to reduce the damage of immunoreaction caused after cerebral I/R injury through AKT/STAT3 pathway. ConclusionsWe evaluated the function and mechanism of lncRNA Neat1 in cerebral I/R injury, And LncRNA Neat1 may be a potential target for new therapeutic intervention.
Effectiveness of recombinant Escherichia coli for the production of (R)-(+)-perillyl alcohol&nbsp;﻿
Authors: Sun Chao , Dong Xianjuan , Zhang Rubing , Xie Congxia 
Published: February 2020

Abstract
Background:(R)-(+)-perillyl alcohol is a naturally oxygenated monoterpene that is produced in perilla leaves, citrus, lemon and lavender. Perillyl alcohols have many uses, including natural flavor additives, insecticides, jet fuels and anti-cancer therapies. The bioconversion of readily available monoterpene precursors, such as (R)-(+)-limonene are recognized as valuable oxygenated derivatives. However, as this natural product is present at low concentrations in plant oils, alternative microbial production methods are required for its extraction from natural plant sources. Results:In this study, we engineered Escherichia coli to possess a heterologous mevalonate (MVA) pathway, including limonene synthase, P-cymene monoxygenase hydroxylase and P-cymene monoxygenase reductase for the production of (R)-(+)-perillyl alcohol. The concentration of (R)-(+)-limonene (the monoterpene precursor to (R)-(+)-perillyl alcohol) reached 27.3 mg/L from glucose. Enhanced (R)-(+)-perillyl alcohol production was therefore achieved. The strain produced (R)-(+)-perillyl alcohol at a titer of 45.7 mg/L in a 5 L bioreactor fed batch system. Conclusions:These data highlight the efficient production of (R)-(+)-perillyl alcohol through microbial fermentation. This can serve as a platform for the future production of other monoterpenes.
Gender-Based Vulnerability: Combining Pareto ranking and geostatistics to model gender-based vulnerability in Rohingya refugee settlements in Bangladesh
Authors: Nelson Erica , Saade Daniela Reyes , Greenough P.Gregg 
Published: February 2020

Abstract
Background: The Rohingya refugee crisis in Bangladesh continues to outstrip humanitarian resources and undermine the health and security of over 900,000 people. Spatial, sector-specific information is required to better understand the needs of vulnerable populations, such as women and girls, and to target interventions with improved efficiency and effectiveness. This study aimed to create a gender-based vulnerability index and explore the geospatial and thematic variations in gender-based vulnerability of Rohingya refugees residing in Bangladesh by utilizing pre-existing, open source data. Methods: Data sources included remotely-sensed REACH data on humanitarian infrastructure, United Nations Population Fund resource availability data, and the Needs and Population Monitoring Survey conducted by the International Organization for Migration in October 2017. Data gaps were addressed through probabilistic interpolation. A vulnerability index was designed through a process of literature review, variable selection and thematic grouping, normalization, and scorecard creation, and Pareto ranking was employed to rank sites based on vulnerability scoring. Spatial autocorrelation of vulnerability was analyzed with the Global and Anselin Local Moran’s I applied to both combined vulnerability index rank and disaggregated thematic ranking. Results: Twenty-four point one percent of settlements were ranked as ‘most vulnerable,’ with 30 highly vulnerable clusters identified predominantly in the upazila of Sadar. Five settlements in Dhokkin, Somitapara, and Pahartoli were categorized as less vulnerable outliers amongst highly vulnerable neighboring sites. Security- and health-related variables appear to be the most significant drivers of gender-specific vulnerability in Cox’s Bazar. Clusters of low security and education vulnerability measures are shown near Kutupalong. Conclusion: The humanitarian sector produces tremendous amounts of data that can be analyzed with spatial statistics to improve research targeting and programmatic intervention. The critical utilization of these data and the validation of vulnerability indexes are required to improve the international response to the global refugee crisis. This study presents a novel methodology that can be utilized to not only spatially characterize gender-based vulnerability in refugee populations, but can also be calibrated to identify and serve other vulnerable populations during crises.
A classroom intervention targeting working memory, attention and language skills: a cluster randomised feasibility trial
Authors: Rowe Anita , Titterington Jill , Holmes Joni , Henry Lucy , Taggart Laurence 
Published: February 2020

Abstract
Background: International debate around the best models of speech and language therapy provision for children with language disorders has highlighted the need for research into classroom-based approaches and intervention dosage. Working memory (WM) is a cognitive skill that underpins attention and language. ‘Recall to Enhance Children’s Attention, Language and Learning’ (RECALL) is a novel, six-week, classroom-based intervention targeting WM to enhance attention and language skills in 4-5 year olds.Methods : A cluster randomised feasibility trial was conducted to address uncertainties around: the feasibility of a definitive trial to evaluate RECALL; the acceptability of RECALL to the health professionals and teachers who would deliver it; and the factors that may impact on the fidelity of its delivery in the classroom. Six classes of 4-5 year olds participated: 2 received RECALL; 2 received an existing intervention targeting attention skills (not underpinned by WM theory); and 2 received education as usual (no intervention). Ten children in each class ( n= 60) were sampled to assess the appropriateness of the outcome measures that may be used in a definitive trial. A process evaluation included: observations of the fidelity of the intervention delivery and semi-structured interviews with the health professionals (HPs) and teachers who delivered RECALL.Results: The recruitment targets were achieved and all six schools completed the trial. For the experimental RECALL intervention, 95% of sessions were delivered but fidelity to the intervention protocol varied between the two schools (76% versus 45%). The interview data revealed mixed findings regarding the acceptability of the intervention tasks and the outcome measures. A greater understanding of the theory underpinning RECALL would have enhanced the teachers’ fidelity to its delivery. Conclusions: The trial processes (e.g., recruitment and consent procedures) could be easily scaled-up into a future definitive trial but the RECALL intervention requires modification to enhance its acceptability. Large class sizes, child and facilitator factors impacted on the dose (number of practice items) accessed by individual children, particularly those most at risk. This study highlights the need for thorough training for teachers and HPs engaged in the delivery of classroom interventions for children with language disorders. Trial registration: ISRCTN13633886 . Registered 7 Sept 2018 . Keywords: working memory, classroom interventions, dosage, working memory, attention, language, feasibility
Isolation, identification and quantification of gallic acid (gallotannins) through HPTLC in leaf galls of Madhuca longifolia (Koenig) j.f. Macb
Authors: Kumkum  , Preeti Mishra  , Rishikesh Meena  , Vidya Patni  
Published: February 2020

Plant galls (cecidia) are pathologically produced cells, tissues, or organs of plants that have developed by hypertrophy and hyperplasia of plant tissues under the effect of gall causing organisms. Madhuca longifolia (Koenig) j.f Macb. is an economically and medicinally very important plant of the family Sapotaceae. It is a tropical mixed deciduous tree. Various galls due to insect infestation are found on almost all tree species. Leaf galls of Madhuca longifolia induced by insects of order Diptera and Hymenoptera represent a unique pattern of chemical perturbations which normally do not occur in normal leaf. During the present investigation, an accurate, fast and easy HPTLC method was followed for quantification of gallic acid occur in the normal leaf and dried leaf galls of the Madhuca longifolia plant. The protocol followed in this study resulted in an intense peak and was able to give a good resolution of gallic acid from normal leaf and galled tissues of Madhuca longifolia (Koenig) j.f. Macb. Adaxial leaf gall induced by insect Mohwadiplosis Orientalis Rao belonging to Diptera class, Marginal gall induced by another Dipteran and vein gall by chalcidoiid Hymenopteran class of insect. Variation in Gallic acid present in normal leaf and leaf galls of Madhuca longifolia was critically reported. It was found that Gallic acid content increased almost two folds in gall tissues as compared to normal leaf tissue. Galled leaf (Dipteran adaxial gall) had a maximum amount of Gallic acid (344.4 ng) while in normal leaf and another leaf galls had less amount of gallic acid was found (180ng). This study is of practical importance because gallic acid is the most important active phenolic acid, which may be correlated with post-infection biochemical defense. Compound gallic acid has been reported for the first time from leaf galls of Madhuca longifolia (Koenig) j. f. Macb. Plant in the study.
PROFIL TAKSONOMI PENGETAHUAN SISWA SMP DALAMPEMECAHAN MASALAH SOAL CERITA SISTEM PERSAMAAN LINEAR DUA VARIABEL DITINJAU DARI GAYA KOGNITIF
Authors: Patta Rahmawati 
Published: February 2020

The Study aimed at discovering (1) the profile of knowledge taxonomy of student in solving story problem of linear equation system of two variables based on cognitive style, (2) the comparison of knowledge taxonomy of student in solving story problem of linear equation system of two variables based on cognitive style.The Study is a qualitative research. The instrument of the study was the researcher himself as the main instrument guided by tasks in solving story problem of linear equation system of two variables and a valid, reliable guided interview. Data were collected by conducting task based interview. The subjects of the study were two grade VIII students of SMPN 5 Marioriwawo in Soppeng district consisted of one student who categorized as field independent named as SFI (subject field independent) and one student who categorized as field dependent named as SFD (subject field dependent).
Menumbuhkan Wirausaha Baru Bagi Mahasiswa dilingkungan UNIBA Surakarta
Authors: Dewi Riana Rachmawati , Annisa Nenden Nur , Suryo Ibnu 
Published: February 2020

Tujuan dari pengabdian ini adalah untuk menumbuhkan minat berwirausaha bagi mahasiswa di UNIBA Surakarta. Metode yang dipergunakan berupa tahapan kegiatan adalah Observasi dan survey, pengumpulan materi, modelling, diskusi/konsultasi, monitoring dan terakhir evaluasi. Hasilnya, sebanyak 2 (dua) kelompok mahasiswa mulai memberanikan diri untuk membuat usaha makanan (kue) dan sesuai dengan rencana awal maka kedua kelompok mahasiswa tersebut mendapatkan bantuan / stimulan dana sebesar Rp. 750.000,- per kelompok. Total dana yang dikeluarkan untuk praktek mahasiswa berwirausaha sebesar Rp. 1.500.000,-. Simpulan, perlu upaya yang berkelanjutan untuk dapat menumbuhkan jiwa wirausaha dikalangan mahasiswa. Upaya modelling ini dilakukan untuk memberikan semangat kepada mahasiswa yang berani untuk mencoba dan memulai (start-up) hal yang baru dan berasal dari hobi dapat dikatakan masih mampu untuk memotivasi mahasiswa memulai bisnis.
Pelatihan Penulisan Proposal Penelitian Tindakan Kelas pada Guru MTs Pesantren DDI Kaballangangg
Authors: Thalhah Sitti Zuhaerah , Raupu Sumardin , Arifanti Dwi Risky 
Published: February 2020

Salah satu upaya dalam meningkatkan kualitas pembelajaran oleh seorang guru profesional adalah melakukan Penelitian Tindakan Kelas (PTK). Tujuan Pelatihan penulisan PTK yaitu: 1). Meningkatkan pemahaman para guru tentang PTK, 2). Mengembangkan pengetahuan dan keterampilan Penelitian Tindakan Kelas para guru di MTs Pesantren DDI Kaballangang. Metode yang digunakan dalam pelaksanaan pelatihan ini adalah metode ceramah dan demonstrasi. Metode ceramah sebagai upaya untuk meningkatkan pemahaman guru berupa penjelasan tentang prinsip-prinsip PTK, prosedur pelaksanaan PTK, implementasi PTK, dan penulisan laporan PTK, untuk metode demonstrasi sebagai upaya mengembangkan pengetahuan dan keterampilan para guru berupa latihan identifikasi masalah dalam proses pembelajaran yang dapat dipecahkan melalui PTK, dan rancangan proposal PTK. Kegiatan ini sangat membantu para guru dalam mengembangkan profesinya dengan adanya hasil luaran berupa proposal PTK. Secara umum, hasil pelaksanaan pelatihan ini adalah cukup baik dan reponsif, artinya terjadi tingkat pemahaman para guru tentang PTK. Hal dapat dilihat bahwa 85% guru mampu mengembangkan keterampilan menulis proposal PTK pada saat pelatihan.
PKM Kelompok Guru Bidang Studi Biologi Kota Palopo Untuk Meningkatkan Keterampilan Media Pembelajaran
Authors: Saparuddin Saparuddin , Charmilasari Charmilasari , Syakur Akhmad 
Published: February 2020

tujuan PKM ini adalah memberikan 1) Pelatihan pembuatan media pembelajaran Biologi, 2) Pendampingan pembuatan media pembelajaran biologi 3) Menyediakan sumber referensi bagi guru terkait ragam media pembelajaran biologi dan tata cara pembuatannya. Kegiatan pelatihan dan penyuluhan menghadirkan nara sumber dari dosen yang sesuai dengan disiplin ilmu yang dibutuhkan pada pelatihan pembuatan media pembelajaran. Setiap setelah mendapatkan materi kemudian dilanjutkan dengan pelatihan pembuatan media pembelajaran. Kegiatan ini tidak sekedar pelatihan saja tetapi dilanjutkan dengan pendampingan di sekolah masing-masing. Hasil dari kegiatan PKM menunjukkan bahwa guru-guru sangat antusias dan berpartisipasi serta memiliki keinginan yang kuat untuk tetap melanjutkan program-program yang sangat bermanfaat bagi mereka, seperti pembuatan media pembelajaran berupa awetan, multimedia, dan e- learning. Pemahaman dan keterampilan yang mereka peroleh dilaksanakn dan diterapkan di sekolah masing-masing.
Manajemen Kepala Madrasah dalam Meningkatkan Kompetensi Profesional Guru Pendidikan Agama Islam
Authors: Tamrin Husni , Nuzuar Nuzuar , Dedi Syahrial 
Published: February 2020

The purpose of this study was to determine the management of madrasa principals in improving the professional competence of Islamic religious education teachers. In this study, the authors used a type of qualitative research with a descriptive approach that is research that describes or illustrates the state or status of phenomena. The results of the study, several things that need to be done by the madrasa head include: 1) Knowing the condition / condition of the teacher in the background of environmental, social and economic life, 2) stimulating the teacher's work spirit in various ways, 3) maintaining the availability of facilities needed to develop the ability teacher, 4) increasing teacher participation in the school environment, 5) fostering a sense of family within the school environment, 6) strengthening the relationship of the school with the community, committee and student guardians. Conclusions, the management of madrasas in improving the professional competence of Islamic religious education teachers (PAI) are: 1) involving teachers in training activities, 2) promoting discipline, 3) motivating teachers, 4) supervision. Conclusion, The application of the management of the madrasa head at the MA Al-Manshuriyah Sungai Baung has already been implemented, the professional competence of PAI teachers at the Al-Manshuriyah MA has been very good
Keywords: Principal Management, PAI Teacher Competence
Peran Kepala Sekolah Sebagai Administrator dan Supervisor
Authors: Hamidi Hamidi , Nuzuwar Nuzuwar , Nurmal Ifnaldi 
Published: February 2020

The purpose of this study was to find out how the role of the principal as an administrator and supervisor. The design of this study uses a qualitative descriptive approach. The research subjects consisted of principals, teachers and staff staff at Megang Sakti Public Middle School, Musi Rawas District. The results of the study explained that the principal had applied his main tasks and functions as the principal. The conclusions of this study are that the Principal of Megang Sakti Middle School has made a good work program, this can be seen from school activities, both related to students and with educators and school education.
Keywords: Work Program, Role of Principal
Sistem Pakar IQ dalam Pengelompokan Anak Reterdasi Mental dengan Algoritma K-Means
Authors: Rahayu Novi 
Published: February 2020

Reterdated mental children are children who have an intellectual level below the average. mentally retarded children are children who have intellectual deficiencies or limitations, are below the normal average, so have difficulties in academic, communication, and social tasks, and require special education services. Expert system is one part of artificial intelligence which has recently experienced very rapid development. This study aims to help the Dharma Wanita Extraordinary School to place students and students into a class with groups that are equally backward by applying the K-Means algorithm. The design of this expert system uses Data Flow Diagrams (DFD) and the programming language used to build expert systems in this study is Visual Basic. The results of the expert system built in this study help in diagnosing the level of mental reterdation based on the symptoms given by the user and the level of mental reterdation based on possible values ​​using the K-Means algorithm calculation.
Keywords:Expert System, Mental Retarded Children, K-Means Algorithm.