bluuebunny/crossref_metadata_2025_split huggingface dataset:
Distinctive roles of syntaxin binding protein 4 and its action target, TP63, in lung squamous cell carcinoma: A theranostic study for the precision medicine
Authors: Bilguun Erkhem-Ochir , Kaira Kyoichi , Kawabata-Iwakawa Reika , Rokudai Susumu , Shimizu Kimihiro , Yokobori Takehiko , Oyama Tetsunari , Shirabe Ken , Nishiyama Masahiko 
Published: September 2020

Abstract
Background: Lung squamous cell carcinoma (LSCC) remains a challenging disease to treat, and further improvements in prognosis are dependent upon the identification of LSCC-specific therapeutic biomarkers and/or targets. We previously found that Syntaxin Binding Protein 4 (STXBP4) plays a crucial role in lesion growth and, therefore, clinical outcomes in LSCC patients through regulation of tumor protein p63 (TP63) ubiquitination. Methods: To clarify the impact of STXBP4 and TP63 for LSCC therapeutics, we assessed relevance of these proteins to outcome of 144 LSCC patients and examined whether its action pathway is distinct from those of currently used drugs in in vitro experiments including RNA-seq analysis through comparison with the other putative exploratory targets and/or markers.Results: Kaplan–Meier analysis revealed that, along with vascular endothelial growth factor receptor 2 (VEGFR2), STXBP4 expression signiﬁed a worse prognosis in LSCC patients, both in terms of overall survival (OS, p=0.002) and disease-free survival (DFS, p=0.041). These prognostic impacts of STXBP4 were confirmed in univariate Cox regression analysis, but not in the multivariate analysis. Whereas, TP63 (ΔNp63) closely related to OS (p=0.013), and shown to be an independent prognostic factor for poor OS in the multivariate analysis (p=0.0324). The action pathway of STXBP4 on suppression of TP63 (ΔNp63) was unique: Ingenuity pathway analysis using the knowledge database and our RNA-seq analysis in human LSCC cell lines indicated that 35 pathways were activated or inactivated in association with STXBP4, but the action pathway of STXBP4 was distinct from those of other current drug targets: STXBP4, TP63 and KDR (VEGFR2 gene) formed a cluster independent from other target genes of tumor protein p53 (TP53), tubulin beta 3 (TUBB3), stathmin 1 (STMN1) and cluster of differentiation 274 (CD274: programmed cell death 1 ligand 1, PD-L1). STXBP4 itself appeared not to be a potent predictive marker of individual drug response, but we found that TP63, main action target of STXBP4, might be involved in drug resistance mechanisms of LSCC. Conclusion: STXBP4 and the action target, TP63, could afford a key to the development of precision medicine for LSCC patients.
A sex/gender perspective on interventions to promote children’s and adolescents’ overall physical activity: Results from genEffects systematic review
Authors: Schulze Carolin , Demetriou Yolanda , Emmerling Sandra , Schlund Annegret , Phillips Susan P. , Puil Lorri , Coen Stephanie E. , Reimers Anne K. 
Published: September 2020

Abstract
Background To evaluate the effects of interventions on children’s and adolescents’ overall physical activity (PA) for boys and girls separately and to appraise the extent to which the studies haven taken sex/gender into account.Methods Systematic review and semi-quantitative analysis. Eleven electronic databases were searched to identify all relevant randomized and non-randomized controlled trials. Studies had to report overall PA as the main outcome to be eligible for inclusion in the review. The main outcomes of the studies is a quantified measure of overall PA. Additionally, all studies had to report sex/gender disaggregated overall PA at baseline and/or follow up and/or explain how they dealt with sex/gender during outcome analysis (i.e., sex/gender adjusted analyses) and/or report that there were no differences in the outcome when looking at sex/gender. PRISMA guidelines were followed. Two authors independently screened studies for eligibility and assessed the risk of bias. Semi-quantitative analyses were conducted to evaluate intervention effects, taking into account the extent to which studies have considered sex/gender aspects. To evaluate sex/gender considerations in primary studies, a newly developed sex/gender checklist was used. The study was registered previously (registration number CRD42018109528).Results In total, 97 articles reporting 94 unique studies with 164 outcomes for overall PA were included in the present review. Average sample size was 829 participants, ranging from five to 9,839. Participants’ ages ranged from three to 19 years. Our review shows that overall 35% of PA outcomes had significant effects in increasing overall PA of children and adolescents. Not including single sex/gender studies, 105 out of 120 PA outcomes resulted in same intervention effects for boys and girls. The interventions reported to have similar effects on PA outcomes for boys and girls showed higher quality of reporting sex/gender aspects of measurement instruments, participant flow and intervention content and materials than PA outcomes with effects only in boys or only in girls. Overall, consideration of sex/gender aspects in intervention studies is low.Conclusions There is still a need to address sufficient consideration of sex/gender aspects in developing and implementing interventions in the context of PA.
Multiple intravenous tranexamic acid doses in total knee arthroplasty in patients with rheumatoid arthritis: a randomized controlled study
Authors: Kang Bing-xin , Xu Hui , Gao Chen-xin , Zhong Sheng , Zhang Jing , Xie Jun , Sun Song-tao , Ma Ying-hui , Xu Xi-rui , Zhao Chi , Zhai Wei-tao , Xiao Lian-bo 
Published: September 2020

Abstract
Background: To identify the efficacy and safety of multiple doses of intravenous tranexamic acid (IV-TXA) on perioperative blood loss in patients with rheumatoid arthritis (RA) who have undergone primary unilateral total knee arthroplasty (TKA).Methods: For this single-center, single-blind randomized controlled clinical trial, 10 male and 87 female participants aged 50–75 years, with RA who underwent unilateral primary TKA were recruited. The patients received one dose of 1g IV-TXA was 10 min before skin incision, followed by articular injection 1.5 g TXA after cavity suture during the surgery. The patients were randomly assigned (1:1) into two groups and received an additional single dose of IV-TXA (1 g) for 3 h (group A) or three doses of IV-TXA (1 g) for 3, 6, and 12 h (group B) postoperatively. Primary outcomes were total blood loss (TBL), hidden blood loss (HBL), and maximum hemoglobin (Hb) decrease. Secondary outcomes were transfusion rate and D-dimer levels. All parameters were measured postoperatively during inpatient hospital stay.Results: Between September 2019 and May 2020, 104 participants were randomized, but 7 were lost to follow-up. The mean TBL, HBL, and maximum Hb decrease in group B (506.1 ± 227.0 mL, 471.6 ± 224.0 mL, and 17.5 ± 7.7 g/L, respectively) were significantly lower than those in group A (608.8 ± 244.8 mL, P = 0.035; 574.0 ± 242.3 mL, P = 0.033; and 23.42 ± 9.2 g/L, P = 0.001, respectively). No episode of transfusion occurred. D-dimer level was lower in group B than in group A on postoperative day 1 (P < 0.001), and the incidence of thromboembolic events was similar between the two groups (P > 0.05). Conclusion: In patients with RA, three doses of postoperative IV-TXA further enabled HBL and Hb decrease without increasing the incidence of adverse events in a short period of time after TKA.Trial registration: The trial was registered in Chinese Clinical Trial Registry (ChiCTR1900025013).
Capsaicin analog supplementation is not effective to improve 10-km running time-trial performance in amateur athletes: a randomized, crossover, double-blind and placebo-controlled study
Authors: Morano Ana Elisa von Ah , Padilha Camila S. , Soares Vinicius Aparecido Matos , Machado Fabiana Andrade , Hofmann Peter , Rossi Fabrício E , Lira Fabio 
Published: September 2020

Abstract
Background: To investigate the acute effects of a capsaicin analogue supplement on 10-km time trial performance and physiological responses in amateur athletes. Methods: Twenty-one participants (age = 29.3 ± 5.5 years), completed two randomized, double-blind trials: capsaicin analogue condition [Capsiate (CAP) = 24 mg] or a placebo condition. The participants consumed two doses of 12 mg of capsaicin or placebo capsule 45 minutes before and immediately at the start of each trial. The time required to complete 10-km, lactate concentration, maximum heart rate (HRpeak), and rating of perceived exertion (RPE) were recorded. Results: 10-km time-trial performance (CAP = 45.07 ± 6.41 min vs. placebo = 45.13 ± 6.73, P = 0.828) was not statistically significant different between conditions. No statistically significant differences between conditions were detected for lactate concentration (P = 0.507), HRpeak (P = 0.897) and RPE (P = 0.517). Conclusion: Two doses of 12 mg Capsaicin analogue supplementation did not improve performance and physiological responses in a 10-km running time-trial in amateur athletes.
Clinical efficacy of short-term pre-operative halo-pelvic traction in the treatment of severe spinal deformities complicated with respiratory dysfunction
Authors: Qi Longtao , Xu Beiyu , Li Chunde , Wang Yu 
Published: September 2020

Abstract
Background Halo traction has been used as an adjunctive method in the treatment of severe spinal deformities. But there are few reports on the clinical efficacy of halo-pelvic traction (HPT) in the treatment of severe spinal deformities complicated with respiratory dysfunction. This study was to evaluate the clinical efficacy and complications associated with pre-operative HPT in the treatment of severe spinal deformities with respiratory dysfunction.Methods Thirty patients with severe spinal deformities complicated with respiratory dysfunction treated with short-term pre-operative HPT were retrospectively reviewed. Inclusion criteria were: (1) patients with severe kyphoscoliosis (coronal Cobb angle or kyphosis angle ≥100°) and respiratory failure, (2) patients undergoing HPT until posterior fusion surgery. All patients underwent general anesthesia for HPT application, which the pelvic ring used in this study was a half-ring, and the rods were all placed on the anterolateral side of the truck. Results The major coronal curve scoliosis averaged 116.00 ± 16.70° and was reduced to 63.23 ± 14.00° after HPT, 46.33 ± 10.70° after surgery. The major kyphosis was 102.40 ± 27.67° and was reduced to 52.23 ± 14.16° after HPT, 42.0 ± 11.92° after surgery. A signiﬁcantly increased FVC was observed after HPT (p<0.001), with a signiﬁcantly improved FVC% (p<0.001). Similarly, a signiﬁcantly increased FEV1 was also observed (p <0.001), with a signiﬁcantly improved FEV1% (p<0.001).Conclusion This study indicated that the modified HPT could be used to help patients with severe spinal deformities complicated with respiratory dysfunction achieve significant correction in both the coronal and sagittal deformities during the pre-operative treatment period along with improved respiratory function and in the absence of severe complications.
Are preoperative high soluble programmed death ligand 1 levels responsible for patients’ poor prognoses in hepato-biliary-pancreatic cancer?
Authors: Okada Rei , Otsuka Yuichiro , Tsuchiya Masaru , Maeda Tetsuya , Ishii Jun , Kubota Yoshihisa , Funahashi Kimihiko , Kaneko Hironori , Shimada Hideaki 
Published: September 2020

Abstract
Background: Several reports showed that high soluble programmed death-ligand 1(sPD-L1) level was a risk factor for poor prognosis in various tumors. To date, the clinicopathologic and prognostic impact of sPD-L1 level in patients with hepato-biliary-pancreatic cancer have not been determined. Methods: A total of 119 patients (66 patients with hepatocellular carcinoma, 23 patients with cholangiocarcinoma, 30 patients with pancreatic cancer) who were treated at the Toho University Omori Hospital (Tokyo, Japan) from 2008 to 2016 were retrospectively analyzed. sPD-L1 levels were measured using an enzyme-linked immunosorbent assay for PD-L1 to evaluate clinicopathologic and prognostic impact. Results: sPD-L1 levels were significantly higher in low-albumin group than normal albumin group. According to stages in hepatocellular carcinoma and cholangiocarcinoma, there were no significant differences in sPD-L1 levels, which gradually increased according to stage in pancreatic cancer. Using a cut-off value of 81.6pg/ml for sPD-L1level, the high sPD-L1 group showed significantly worse prognosis than the low sPD-L1 group in patients with pancreatic cancer. Multivariate analysis identified sPD-L1 level ≥ 81.6 mg/dl ( p = 0.047) as an independent predictor of poor overall survival in patients with pancreatic cancer.Conclusion: Using a cut-off value of 81.6pg/ml for sPD-L1level, high sPD-L1 levels were independently associated with poor prognosis in patients with pancreatic cancer. However, this association in hepatocellular carcinoma or cholangiocarcinoma was not clear.
Bioretention As A Control To Urban Drainage System With An Ecohydrological Base: GIS As A Tool On Decision Making
Authors: Rosa Altair , Procopiuck Mario , Macedo Marina Batalini de , Lago César Ambrogi Ferreira do , Sample David , Souza Vladimir Caramori de , Mendiondo Mario Eduardo 
Published: September 2020

Abstract
The occupation and use of increasingly impermeable urban land have made it difficult to infiltrate water and, consequently, increase the volume of runoff in different cities, which has required the development of bioretention techniques in the field of hydrology. The aim of this article is to define and apply criteria for the identification of areas for the construction of Bioretention systems for evaluations based on Geographic Information System indicators, considering the aspects of quantity and quality in urban drainage . The developed method allows to verify and compare changes in the surface of urban areas and their interference in the local environment , the mapping of land use and occupation to simplify procedures to define and prioritize areas for the construction of Bioretention systems, the use of resources from georeferenced bases to resolve eco-hydrological issues. The study develops technical bases for the use of a georeferencing tool to analyze areas with speed and consistency as a basis for decisions on the implementation of Bioretention systems
Fine structure of the intercalated disc and cardiac junctions in the black widow spider Latrodectus mactans
Authors: SUN Yan , LEE Seung-Min , KU Bon-Jin , Moon Myung-Jin 
Published: September 2020

Abstract
Arthropods have an open circulatory system with a simple tubular heart, so it has been estimated that the contractile pumping structure of the cardiac muscle will be less efficient than that of vertebrates. Nevertheless, certain arthropods are known to have far superior properties and characteristics than vertebrates, so we investigated the fine structural features of intercalated discs and cardiac junctions of cardiac muscle cells in the black widow spider Latrodectus mactans. Characteristically, the spider cardiac muscle has typical striated features and represents a functional syncytium that supports multiple connections to adjacent cells by intercalated discs. Histologically, the boundary lamina of each sarcolemma connects to the basement membrane to form an elastic sheath, and the extracellular matrix allows the cells to be anchored to other tissues. Since the intercalated disc is also part of sarcolemma, it contains gap junctions for depolarization and desmosomes that keep the fibers together during cardiac muscle contraction. Furthermore, fascia adherens and macula adherens (desmosomes) were also identified as cell junctions in both sarcolemma and intercalated discs. To enable the coordinated heartbeat of the cardiac muscle, the muscle fibers have neuronal innervations by multiple axons from the motor ganglion.
Differential kynurenine pathway metabolism in highly-metastatic aggressive breast cancer subtypes: beyond IDO1-induced immunosuppression
Authors: Heng Benjamin , Bilgin Ayse A. , Lovejoy David B , Tan Vanessa X , Milioli Heloisa Helena , Gluch Laurence , Busamante Sonia , Sabaretnam Tharani , Moscato Pablo , Lim Chai K , Guillemin Gilles J 
Published: September 2020

Abstract
Background: Immunotherapy has recently been proposed as a promising treatment to stop breast cancer (BrCa) progression and metastasis. However, there has been limited success in the treatment of BrCa with immune checkpoint inhibitors. This implies that BrCa tumors have other mechanism(s) to escape immune surveillance. While the kynurenine pathway (KP) is known to be a key player mediating tumor immune evasion and while there are several studies on the roles of the KP in cancer, little is known about KP involvement in BrCa.Methods: To understand how KP is regulated in BrCa, we examined the KP profile in BrCa cell lines and clinical samples (n = 1,997) that represent major subtypes of BrCa (luminal, HER2-enriched, and triple-negative (TN)). We carried out qPCR, western blot/immunohistochemistry and ultra-high pressure liquid chromatography on these samples to quantify KP enzyme gene, protein and activity respectively.Results: We revealed that the KP is highly dysregulated in the HER2-enriched and TN BrCa subtype. Gene, protein expression and KP metabolomic profiling have shown that the downstream KP enzymes KMO and KYNU are highly upregulated in the HER2-enriched and TN BrCa subtypes, leading to increased production of the potent immunosuppressive metabolites anthranilic acid (AA) and 3-hydroxylanthranilic acid (3HAA).Conclusions: Our findings suggest that KMO and KYNU inhibitors may represent new promising therapeutic targets for BrCa. We also showed that KP metabolite profiling can be used as an accurate biomarker for BrCa subtyping, as we successfully discriminated TN BrCa from other BrCa subtypes.
Improving Retention of Community-recruited Participants in HIV Prevention Research Through Saturday Household Visits; Findings from the HPTN 071 (PopART) Study in South Africa.
Authors: Bell-Mandla Nomtha Fundiswa , Sloot Rosa , Maarman Gerald , Griffith Sam , Moore Ayana , Floyd Sian , Hayes Richard , Fidler Sarah , Ayles Helen , Bock Peter 
Published: September 2020

Abstract
BackgroundIdentifying successful strategies to improve participant retention in longitudinal studies remains a challenge. In this study we evaluated whether non-traditional fieldworker shifts (after hours during the week and weekends) enhanced participant retention when compared to retention during traditional weekday shifts in the HPTN 071 (PopART) population cohort (PC). MethodsHPTN 071 (PopART) PC participants were recruited and followed up in their homes on an annual basis by research fieldworkers over a 3-4 year period. The average number of successful follow-up visits, where a PC participant was found and retained in the study, was calculated for each of 3 visit schedules (early weekday shift, late weekday shift, and Saturday shift), and standardized to account for variation in fieldwork shift duration. We used one-way univariate analysis of variance (ANOVA) to describe differences in mean-successful visits and 95% confidence intervals between the shift types.ResultsData on 16651 successful visits were included. Successful visit rates were higher when conducting Saturday visits (14.0; 95% CI: 11.3-16.6) compared to both regular (4.5; 95% CI: 3.7-5.3) and late weekday shifts (5.3; 95% CI: 4.7-5.8) overall and in all subgroup analyses (P<0.001). The successful visit rate was higher amongst women than men were during all shift types (3.2 vs. 1.3, p<0.001). Successful visit rates by shift type did not differ significantly by age, over time, by PC round or by community triplet. ConclusionThe number of people living with HIV continues to increase annually. High quality evidence from longitudinal studies remains critical for evaluating HIV prevention and treatment strategies. This study showed a significant benefit on participant retention through introduction of Saturday shifts for home visits and these data can make an important contribution to the emerging body of evidence for improving retention in longitudinal research. Trial registrationPopART was approved by the Stellenbosch University Health Research Ethics Committees (N12/11/074), London School of Hygiene and Tropical Medicine (6326) ethics committee and the Division of AIDS (DAIDS) (Protocol ID 11865). PopART was registered with ClinicalTrials.gov (registration number NCT01900977).
Designing a Permissioned Blockchain Network for the Halal Industry Using Hyperledger Fabric with Multiple Channels and the Raft Consensus Mechanism
Authors: Surjandari Isti , Yusuf Harman , Laoh Enrico , Maulida Rayi 
Published: September 2020

Abstract
Blockchain is an alternative solution that can improve the performance of the halal supply chain. Blockchain can address all the issues in the halal supply chain, such as the contamination of halal products and the disobedience of halal processes among all parties in the chain, including end customers. The type of technology suitable for this case is permissioned blockchain, in which administrators can determine the rights of each halal supply chain participant category. The determination of these rights must be done by consensus so that no party feels aggrieved. This study uses a blockchain network with three channels and the Raft consensus algorithm to design a web interface and test its capabilities. With regard to the web interface, there were no validity failures during the invoke and query tests. In addition, the web interface was also successful in thwarting the formation of a block in the case of data input errors from the user. Additionally, the server can act as a provider of information and validator of the web interface. The results of simulations conducted on the blockchain network show that the system’s transaction speed is fast, and all the transactions are successfully transferred to other peers. Thus, permissioned blockchain is useful for the halal supply chain not just because it can secure transactions while addressing halal issues but also because the transaction speed and rate of transfer data are very effective.
Unconventional reconciliation path for quantum mechanics and general relativity
Authors: Yuguru Samuel 
Published: September 2020

Abstract
Physics in general is successfully governed by quantum mechanics at the microscale and principles of relativity at the macroscale. Any attempts to unify them using conventional methods have somewhat remained elusive for nearly a century up to the present stage. Here in this study, a classical gedanken experiment of electron-wave diffraction of a single slit is intuitively examined for its quantized states. A unidirectional monopole field as quanta of the electric field is pictorially conceptualized. Its application towards quantum mechanics and general relativity in accordance with existing knowledge in physics paves an alternative path towards their reconciliation process by assuming a multiverse at a hierarchy of scales. Such an outcome provides an approximate intuitive guide to examine physics in general from alternative perspectives using conventional methods.