bluuebunny/crossref_metadata_2025_split huggingface dataset:
Inflammation and cardiovascular diseases: lessons from seminal clinical trials
Authors: Liberale Luca , Montecucco Fabrizio , Schwarz Lena , Lüscher Thomas F , Camici Giovanni G 
Published: July 2020

AbstractInflammation has been long regarded as a key contributor to atherosclerosis. Inflammatory cells and soluble mediators play critical roles throughout arterial plaque development and accordingly, targeting inflammatory pathways effectively reduces atherosclerotic burden in animal models of cardiovascular (CV) diseases. Yet, clinical translation often led to inconclusive or even contradictory results. The Canakinumab Anti-inflammatory Thrombosis Outcome Study (CANTOS) followed by the Colchicine Cardiovascular Outcomes Trial (COLCOT) were the first two randomized clinical trials to convincingly demonstrate the effectiveness of specific anti-inflammatory treatments in the field of CV prevention, while other phase III trials—including the Cardiovascular Inflammation Reduction Trial one using methotrexate—were futile. This manuscript reviews the main characteristics and findings of recent anti-inflammatory Phase III trials in cardiology and discusses their similarities and differences in order to get further insights into the contribution of specific inflammatory pathways on CV outcomes. CANTOS and COLCOT demonstrated efficacy of two anti-inflammatory drugs (canakinumab and colchicine, respectively) in the secondary prevention of major adverse CV events (MACE) thus providing the first confirmation of the involvement of a specific inflammatory pathway in human atherosclerotic CV disease (ASCVD). Also, they highlighted the NOD-, LRR-, and pyrin domain-containing protein 3 inflammasome-related pathway as an effective therapeutic target to blunt ASCVD. In contrast, other trials interfering with a number of inflammasome-independent pathways failed to provide benefit. Lastly, all anti-inflammatory trials underscored the importance of balancing the risk of impaired host defence with an increase in infections and the prevention of MACE in CV patients with residual inflammatory risk.
Making Her Time (and Time Again): Feminist Phenomenology and Form in Recent British and Irish Fiction Written by Women
Authors: Gray Madeleine 
Published: July 2020

AbstractThis article reads Ali Smith’s 2014 novel How to Be Both alongside Deborah Levy’s Hot Milk (2016) and Sally Rooney’s Conversations with Friends (2017). Using Lauren Berlant’s conception of neoliberal “crisis subjectivity” and Sara Ahmed’s vision of feminist wonderment as an antidote to the neoliberal “promise of happiness,” it argues that each novel considers what might be salvaged and what might grow from situations in which young women become attuned to their mutual incarceration in neoliberal time’s double bind. It contends that the forced improvisation and feminist reorientation undertaken by the protagonists can be analyzed through the lens of temporality. Finally, it contemplates how the disciplinary-specific modes through which the novels’ protagonists consider temporality might be connected to the specific literary projects of their authors.
Insights into the evolution of symbiosis gene copy number and distribution from a chromosome-scaleLotus japonicusGifu genome sequence
Authors: Kamal Nadia , Mun Terry , Reid Dugald , Lin Jie-Shun , Akyol Turgut Yigit , Sandal Niels , Asp Torben , Hirakawa Hideki , Stougaard Jens , Mayer Klaus F X , Sato Shusei , Andersen Stig Uggerhøj 
Published: July 2020

AbstractLotus japonicus is a herbaceous perennial legume that has been used extensively as a genetically tractable model system for deciphering the molecular genetics of symbiotic nitrogen fixation. Our aim is to improve the L. japonicus reference genome sequence, which has so far been based on Sanger and Illumina sequencing reads from the L. japonicus accession MG-20 and contained a large fraction of unanchored contigs. Here, we use long PacBio reads from L. japonicus Gifu combined with Hi-C data and new high-density genetic maps to generate a high-quality chromosome-scale reference genome assembly for L. japonicus. The assembly comprises 554 megabases of which 549 were assigned to six pseudomolecules that appear complete with telomeric repeats at their extremes and large centromeric regions with low gene density. The new L. japonicus Gifu reference genome and associated expression data represent valuable resources for legume functional and comparative genomics. Here, we provide a first example by showing that the symbiotic islands recently described in Medicago truncatula do not appear to be conserved in L. japonicus.
Optimizing Parasitoid and Host Densities for Efficient Rearing of Ontsira mellipes (Hymenoptera: Braconidae) on Asian Longhorned Beetle (Coleoptera: Cerambycidae)
Authors: Wang Xingeng , Aparicio Ellen M , Duan Jian J , Gould Juli , Hoelmer Kim A 
Published: July 2020

Abstract
Ontsira mellipes Ashmead is a gregarious larval ectoparasitoid of woodboring cerambycids. It is native to North America but can readily attack the exotic Asian longhorned beetle, Anoplophora glabripennis (Motschulsky). This study aimed to develop an efficient rearing system for this parasitoid, as a potential novel association biocontrol agent for the beetle, by investigating the effects of different densities of host (two, three, or four larvae) and parasitoid (one, two, three, four, five, six, seven, and eight female wasps) on Ontsira’s parasitization efficiency and reproductive outcomes. Results showed that overall parasitism and total numbers of parasitized hosts or progeny produced increased with host and/or parasitoid densities, but the number of parasitized hosts or progeny produced per female parasitoid decreased with parasitoid density at each given host density. Nonlinear regression indicated a consistent pattern of mutual interference as parasitoid density increased. Additional experiments showed that superparasitism (indirect interference) did not occur probably because the parasitoid detects hosts through vibration cues from host feeding and attacked (thus paralyzed) hosts are no longer detectable. Thus, the interference probably results from direct or exploitative competition. Interestingly, female parasitoids responded to increased parasitoid density with a significant increase in clutch size. Overall, per capita parasitization efficiency or reproductive outcomes were optimized at a low parasitoid–host ratio but with large group size of hosts and parasitoids. Therefore, an optimal combination of exposing three or four parasitoids to four hosts is proposed for efficient mass-rearing of this parasitoid.
Methanotroph populations and CH4 oxidation potentials in high-Arctic peat are altered by herbivory induced vegetation change
Authors: Rainer Edda M , Seppey Christophe V W , Tveit Alexander T , Svenning Mette M 
Published: July 2020

ABSTRACT
Methane oxidizing bacteria (methanotrophs) within the genus Methylobacter constitute the biological filter for methane (CH4) in many Arctic soils. Multiple Methylobacter strains have been identified in these environments but we seldom know the ecological significance of the different strains. High-Arctic peatlands in Svalbard are heavily influenced by herbivory, leading to reduced vascular plant and root biomass. Here, we have measured potential CH4 oxidation rates and identified the active methantrophs in grazed peat and peat protected from grazing by fencing (exclosures) for 18 years. Grazed peat sustained a higher water table, higher CH4 concentrations and lower oxygen (O2) concentrations than exclosed peat. Correspondingly, the highest CH4 oxidation potentials were closer to the O2 rich surface in the grazed than in the protected peat. A comparison of 16S rRNA genes showed that the majority of methanotrophs in both sites belong to the genus Methylobacter. Further analyses of pmoA transcripts revealed that several Methylobacter OTUs were active in the peat but that different OTUs dominated the grazed peat than the exclosed peat. We conclude that grazing influences soil conditions, the active CH4 filter and that different Methylobacter populations are responsible for CH4 oxidation depending on the environmental conditions.
Participatory learning and action cycles with women’s groups to prevent neonatal death in low-resource settings: A multi-country comparison of cost-effectiveness and affordability
Authors: Pulkki-Brännström Anni-Maria , Haghparast-Bidgoli Hassan , Batura Neha , Colbourn Tim , Azad Kishwar , Banda Florida , Banda Lumbani , Borghi Josephine , Fottrell Edward , Kim Sungwook , Makwenda Charles , Ojha Amit Kumar , Prost Audrey , Rosato Mikey , Shaha Sanjit Kumer , Sinha Rajesh , Costello Anthony , Skordis Jolene 
Published: July 2020

AbstractWHO recommends participatory learning and action cycles with women’s groups as a cost-effective strategy to reduce neonatal deaths. Coverage is a determinant of intervention effectiveness, but little is known about why cost-effectiveness estimates vary significantly. This article reanalyses primary cost data from six trials in India, Nepal, Bangladesh and Malawi to describe resource use, explore reasons for differences in costs and cost-effectiveness ratios, and model the cost of scale-up. Primary cost data were collated, and costing methods harmonized. Effectiveness was extracted from a meta-analysis and converted to neonatal life-years saved. Cost-effectiveness ratios were calculated from the provider perspective compared with current practice. Associations between unit costs and cost-effectiveness ratios with coverage, scale and intensity were explored. Scale-up costs and outcomes were modelled using local unit costs and the meta-analysis effect estimate for neonatal mortality. Results were expressed in 2016 international dollars. The average cost was $203 (range: $61–$537) per live birth. Start-up costs were large, and spending on staff was the main cost component. The cost per neonatal life-year saved ranged from $135 to $1627. The intervention was highly cost-effective when using income-based thresholds. Variation in cost-effectiveness across trials was strongly correlated with costs. Removing discounting of costs and life-years substantially reduced all cost-effectiveness ratios. The cost of rolling out the intervention to rural populations ranges from 1.2% to 6.3% of government health expenditure in the four countries. Our analyses demonstrate the challenges faced by economic evaluations of community-based interventions evaluated using a cluster randomized controlled trial design. Our results confirm that women’s groups are a cost-effective and potentially affordable strategy for improving birth outcomes among rural populations.
Beyond Description: The Many Facets of Dental Biomechanics
Authors: Crofts S B , Smith S M , Anderson P S L 
Published: July 2020

Synopsis
Teeth lie at the interface between an animal and its environment and, with some exceptions, act as a major component of resource procurement through food acquisition and processing. Therefore, the shape of a tooth is closely tied to the type of food being eaten. This tight relationship is of use to biologists describing the natural history of species and given the high instance of tooth preservation in the fossil record, is especially useful for paleontologists. However, correlating gross tooth morphology to diet is only part of the story, and much more can be learned through the study of dental biomechanics. We can explore the mechanics of how teeth work, how different shapes evolved, and the underlying forces that constrain tooth shape. This review aims to provide an overview of the research on dental biomechanics, in both mammalian and non-mammalian teeth, and to synthesize two main approaches to dental biomechanics to develop an integrative framework for classifying and evaluating dental functional morphology. This framework relates food material properties to the dynamics of food processing, in particular how teeth transfer energy to food items, and how these mechanical considerations may have shaped the evolution of tooth morphology. We also review advances in technology and new techniques that have allowed more in-depth studies of tooth form and function.
The learning curve of video-assisted mediastinoscopic lymphadenectomy for staging of non-small-cell lung carcinoma
Authors: Daemen Jean H T , van den Broek Robert A M , Lozekoot Pieter W J , Maessen Jos G , Hulsewé Karel W E , Vissers Yvonne L J , de Loos Erik R 
Published: July 2020

Abstract

OBJECTIVES
The objective of this study was to define the learning process of video-assisted mediastinoscopic lymphadenectomy (VAMLA) by the assessment of consecutive procedural metrics.

METHODS
We conducted a single-centre retrospective observational study of all consecutive VAMLAs performed between 2011 and 2018 for the staging of non-small-cell lung carcinoma. Learning curves were assessed using non-risk adjusted cumulative observed minus expected (CUSUM) failure charts of complications. Boundary lines were defined by the acceptable and unacceptable complication rates of 4.5% and 15.0%. The Kruskal–Wallis test with post hoc analysis was used to assess trends in operation time and blood loss.

RESULTS
Two-hundred-thirty-six unique VAMLAs by 4 surgeons performing their first procedures were evaluated. CUSUM charts of surgeons A and B showed a typical learning curve with an initial incline, followed by a turning point towards lower complications rates after 16–17 cases, whereas surgeons C and D showed an average performance. The median time between consecutive VAMLAs was shorter for surgeons A and B (13.0 vs 28.5–38.0 days for surgeons C and D). Overcoming the learning curve, complication rates of surgeons A and B decreased from 19% to 3% and from 18% to 5%, respectively. Operation time and blood loss showed a significant improvement after, respectively, 81–100 and 61–80 procedures compared to the first 20 procedures.

CONCLUSIONS
VAMLA is a safe procedure to adopt and perform with acceptable complication rates from the first operation onward, regardless of the caseload. To overcome its learning curve, 16–17 cases are required, preferably at least 1 per 2 weeks.
Rethinking Transitional Justice in South Sudan: Critical Perspectives on Justice and Reconciliation
Authors: Akech Joseph Geng 
Published: July 2020

Abstract
The horrific violations committed in South Sudan’s civil war led to the establishment of mechanisms ensuring the prosecution of serious crimes and reconciliation within communities shattered by war. Through a peace deal, three mutually supportive but independent institutions of transitional justice were formed: The Commission for Truth, Reconciliation and Healing, The Hybrid Court for South Sudan and The Compensation and Reparation Authority. This article examines the extent to which these mechanisms can achieve their respective aims, and argues that prosecutions need to be implemented in an integrated manner alongside reconciliation and healing because of the massive scale of abuses and enormous number of perpetrators. The article further contends that their collaboration with local, indigenous justice mechanisms is crucial because of the latter’s extensive experience in facilitating accountability and reconciliation within divided communities in South Sudan. The article concludes that whilst a limited amnesty is inevitable, a blanket amnesty is counterproductive to the aims of transitional justice in post-conflict South Sudan. Further, the article cautions against the use of immunity, arguing that this would deny justice to victims and could lead to a rise in revenge or private retribution, lawlessness and impunity.
The Importance of Cellular Immunity in the Development of Vaccines and Therapeutics for COVID-19
Authors: Schlom Jeffrey , Donahue Renee N 
Published: July 2020

AbstractIt is important to develop vaccines that can also mediate T-cell responses to SARS-CoV-2 to limit severity of infections, and to analyze the cellular immunome in the use of anti-SARS-CoV-2 therapeutics.
Feeding a calf starter containing monensin alone or in combination with an oregano, and cobalt blend to Holstein calves
Authors: Wu Jianping , Guo Jinsheng , Liu Ting , Chen Hao , Bai Yan , Casper David P 
Published: July 2020

Abstract
Gut health is critically important for growing neonatal calves, and nutritional technologies are needed to prevent disease and stress challenges. Previous work feeding monensin (MON) in combination with an oregano, prebiotic, and cobalt-lactate (EOC) blend had demonstrated improved calf gut health and growth performance. The objective of this study was to evaluate the growth performance of calves fed MON and EOC alone or in combination. Eighty (80) newborn Holstein (37) female and (43) male calves were randomly assigned to one of four treatments arranged in a 2 × 2 factorial (MON and EOC). Treatments were: 1) Control: without MON or EOC added to the calf starter (CS); 2) MON: 50.8 mg/kg CS (Elanco, Greenfield, IN); 3) EOC: 44.1 mg/kg CS (Rum-A-Fresh, Ralco Inc. Marshall, MN); 4) MON + EOC: MON and EOC added to CS. Calves were fed colostrum followed by whole milk through weaning at 42 d, while CS was fed ad libitum through the 70-d experimental period. The MON by EOC interaction was found to be nonsignificant (P &gt; 0.41) for growth performance. Calves fed without or with MON demonstrated similar (P &gt; 0.70) body weight (BW; 68.7 and 68.9 kg without and with MON, respectively), while calves fed EOC demonstrated greater (P &lt; 0.01) BW (67.3 and 70.4 kg without and with EOC, respectively) compared with calves fed without EOC. Calves fed a CS containing MON were similar (P &gt; 0.47) in average daily gain (ADG; 0.88 and 0.91 kg/d) compared with calves fed without MON; however, feeding calves a CS with EOC increased (P &lt; 0.01) ADG (0.84 and 0.95 kg/d) by 13% through the 70-d experimental period compared with calves not fed EOC. Frame measurements indicated that the greater ADG was due to increased (P &lt; 0.10) frame growth for calves fed essential oils (EO) compared with calves fed without EO. A MON by EOC interaction (P &lt; 0.01) for serum propionate concentration demonstrated calves fed MON + EOC and EOC were greater (P &lt; 0.05) compared with calves fed Control, while calves fed MON were intermediate and different (P &lt; 0.05). Feeding calves a CS with EOC increased (P &lt; 0.04) immunoglobulin A, immunoglobulin G, and immunoglobulin M concentrations compared with calves fed without EOC. A MON by EOC interaction was detected (P &lt; 0.01) for total tract starch digestibility for calves fed EOC or MON + EOC demonstrating greater (P &lt; 0.05) starch digestibilities than Control-fed calves. These data demonstrate that EOC and MON fed in combination was not beneficial for enhancing the growth performance, but that calf growth performance can be improved with EOC compared with MON.
Exosomes derived from chronic lymphocytic leukaemia cells transfer miR-146a to induce the transition of mesenchymal stromal cells into cancer-associated fibroblasts
Authors: Yang Yanli , Li Jun , Geng Yinghua 
Published: July 2020

Abstract
Chronic lymphocytic leukaemia (CLL) is the most prevalent leukaemia and remains incurable. Mesenchymal stem cells (MSCs) can promote tumour progression by differentiating into cancer-associated fibroblasts (CAFs). However, the mechanisms by which tumour cells induce the transition of MSCs to CAFs are still largely undefined. Exosomes can regulate recipient cellular function by mediating intracellular communication. This study aimed to investigate whether CLL cells regulate the transition of bone marrow-derived MSCs (BM-MSCs) to CAFs via exosomal miR-146a delivery. The exosomes were isolated from CLL cell line MEC-1 (CLL-Exo) and then co-cultured with BM-MSCs. The expression of α-smooth muscle actin (α-SMA) and fibroblast-activated protein (FAP) were determined by immunofluorescence, quantitative real-time polymerase chain reaction and western blot. A luciferase reporter assay was performed to verify whether ubiquitin-specific peptidase 16 (USP16) was a target of miR-146a. CLL-Exo treatment up-regulated miR-146a and down-regulated expression of CAF markers (α-SMA and FAP) and USP16. The inducing effect of CLL-Exo on CAF marker expression was compromised when miR-146a expression was inhibited in CLL-Exo. USP16 was confirmed as a direct target of miR-146a and USP16 overexpression in BM-MSCs abrogated the CLL-Exo-mediated up-regulation of CAF markers. Collectively, CLL-Exo delivered miR-146a into BM-MSCs where miR-146a mediated transition of BM-MSCs into CAFs by targeting USP16.
Decline in Sublethal Effects of Bt Corn on Corn Earworm (Lepidoptera: Noctuidae) Linked to Increasing Levels of Resistance
Authors: Reay-Jones Francis P F , Bilbo Thomas R , Reisig Dominic D 
Published: July 2020

Abstract
As part of an ongoing longitudinal study in North and South Carolina, this study reports the recovery of Helicoverpa zea (Boddie) pupae in field trials with genetically engineered corn, Zea mays L., hybrids that produce insecticidal toxins from Bacillus thuringiensis (Bt) in 2017–2019. In total, 10,400 corn ears were collected, which led to 3,927 H. zea pupae (2,215 in South Carolina and 1,712 in North Carolina). Late-planted corn led to a 3.39-fold increase in recovery of pupae compared to early-planted corn. Bt corn expressing Cry1F + Cry1Ab and Cry1A.105 + Cry2Ab2 had 1.67-fold and 2.51-fold fewer pupae than non-Bt near-isolines, respectively. Only six pupae were recovered from the hybrid expressing Cry1F + Cry1Ab + Vip3Aa20. Averaged across trials, Bt corn expressing either Cry1A.105 + Cry2Ab2 or Cry1F + Cry1Ab significantly reduced pupal weight compared to non-Bt near-isolines in North and South Carolina. Combining our data with a previous study at the same locations (Bilbo et al. 2018), reduction in pupal weight between Bt and non-Bt near-isolines significantly declined from 2014 to 2019 for Cry1Ab + Cry1F in North and South Carolina. This decline in levels of a sublethal effect of Bt corn expressing Cry1Ab + Cry1F on H. zea at both locations is likely correlated with resistance development.
Cumulation Across Attitudes and Plural Projection
Authors: Schmitt Viola 
Published: July 2020

AbstractThis paper investigates cumulative readings of sentences in which some, but not all of the plural expressions have a de dicto reading, i.e. sentences where the lower plural is interpreted in the scope of an attitude verb like believe. I argue that such cases represent a problem for existing accounts of cumulativity, because the required cumulative relation cannot be formed. I then motivate and propose an alternative analysis where all plural expressions are interpreted in situ: I expand the ‘plural projection’ framework put forth by Haslinger & Schmitt (2018, 2019), Schmitt (2019), where embedded pluralities ‘project’ to the denotations of higher nodes in the sense that the latter reflect the part-structure of the former and where cumulativity is derived via a compositional rule in a step-by-step fashion. I show that if the denotations of the plurals with the de dicto construal are analyzed as pluralities of individual concepts, which project in the afore-mentioned sense to pluralities of propositions, the data can be explained straightforwardly. This proposal differs from treatments in terms of collective belief that don’t appeal to pluralities of propositions ( Pasternak 2018a, b), in that it (i) arguably generalizes to a larger number of examples and (ii) links grammatical plurality in the embedded clause to the availability of cumulative readings.