bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association of ketamine use with lower risks of post-intubation hypotension in hemodynamically-unstable patients in the emergency department
Authors: Ishimaru Tadayoshi , Goto Tadahiro , Takahashi Jin , Okamoto Hiroshi , Hagiwara Yusuke , Watase Hiroko , Hasegawa Kohei ,   Japanese Emergency Medicine Network Investigators, Morita Hiroshi , Kawano Takahisa , Kamikawa Yohei , Nagai Hideya , Matsumoto Takashi , Nonami Suguru , Miyoshi Yusuke , Segawa Sho , Kitai Yuya , Tanaka Kenzo , Minami Saburo , Yakushiji Hiromasa , Okamoto Hiroshi , Miyauchi Naoto , Goto Yukari , Sato Nobuhiro , Gibo Koichiro , Okubo Masashi , Nakayama Yukiko , Miyamae Nobuhiro , Kaoru Hirose , Imamura Taichi , Uendan Azusa , Koyama Yasuaki , Kamura Hiroshi , Yoshiyuki Nakashima , Takahashi Jin , Irie Jin , Okada Nobunaga , Oya Seiro , Inoue Akihiko 
Published: November 2019

AbstractTo determine whether ketamine use for tracheal intubation, compared to other sedative use, is associated with a lower risk of post-intubation hypotension in hemodynamically-unstable patients in the emergency department (ED), we analyzed the data of a prospective, multicenter, observational study—the second Japanese Emergency Airway Network (JEAN-2) Study—from February 2012 through November 2017. The current analysis included adult non-cardiac-arrest ED patients with a pre-intubation shock index of ≥0.9. The primary exposure was ketamine use as a sedative for intubation, with midazolam or propofol use as the reference. The primary outcome was post-intubation hypotension. A total of 977 patients was included in the current analysis. Overall, 24% of patients developed post-intubation hypotension. The ketamine group had a lower risk of post-intubation hypotension compared to the reference group (15% vs 29%, unadjusted odds ratio [OR] 0.45 [95% CI 0.31–0.66] p < 0.001). This association remained significant in the multivariable analysis (adjusted OR 0.43 [95% CI 0.28–0.64] p < 0.001). Likewise, in the propensity-score matching analysis, the patients with ketamine use also had a significantly lower risk of post-intubation hypotension (OR 0.47 [95% CI, 0.31–0.71] P < 0.001). Our observations support ketamine use as a safe sedative agent for intubation in hemodynamically-unstable patients in the ED.
Partial reduced Pi transport function of PiT-2 might not be sufficient to induce brain calcification of idiopathic basal ganglia calcification
Authors: Nishii Kazuya , Shimogawa Ritsuko , Kurita Hisaka , Inden Masatoshi , Kobayashi Michio , Toyoshima Itaru , Taguchi Yoshiharu , Ueda Akihiro , Tamune Hidetaka , Hozumi Isao 
Published: November 2019

AbstractIdiopathic basal ganglia calcification (IBGC) is a rare intractable disease characterized by abnormal mineral deposits, including mostly calcium in the basal ganglia, thalamus, and cerebellum. SLC20A2 is encoding the phosphate transporter PiT-2 and was identified in 2012 as the causative gene of familial IBGC. In this study, we investigated functionally two novel SLC20A2 variants (c.680C > T, c.1487G > A) and two SLC20A2 variants (c.82G > A, c.358G > C) previously reported from patients with IBGC. We evaluated the function of variant PiT-2 using stable cell lines. While inorganic phosphate (Pi) transport activity was abolished in the cells with c.82G > A, c.358G > C, and c.1487G > A variants, activity was maintained at 27.8% of the reference level in cells with the c.680C > T variant. Surprisingly, the c.680C > T variant had been discovered by chance in healthy members of an IBGC family, suggesting that partial preservation of Pi transport activity may avoid the onset of IBGC. In addition, we confirmed that PiT-2 variants could be translocated into the cell membrane to the same extent as PiT-2 wild type. In conclusion, we investigated the PiT-2 dysfunction of four SLC20A2 variants and suggested that a partial reduced Pi transport function of PiT-2 might not be sufficient to induce brain calcification of IBGC.
Analysis of HIV-1 diversity, primary drug resistance and transmission networks in Croatia
Authors: Oroz Maja , Begovac Josip , Planinić Ana , Rokić Filip , Lunar Maja M. , Zorec Tomaž Mark , Beluzić Robert , Korać Petra , Vugrek Oliver , Poljak Mario , Lepej Snježana Židovec 
Published: November 2019

AbstractMolecular epidemiology of HIV-1 infection in treatment-naive HIV-1 infected persons from Croatia was investigated. We included 403 persons, representing 92.4% of all HIV-positive individuals entering clinical care in Croatia in 2014–2017. Overall prevalence of transmitted drug resistance (TDR) was estimated at 16.4%. Resistance to nucleoside reverse transcriptase inhibitors (NRTIs), non-nucleoside RTI (NNRTIs) and protease inhibitors (PIs) was found in 11.4%, 6.7% and 2.5% of persons, respectively. Triple-class resistance was determined in 2.2% of individuals. In addition, a single case (1.0%) of resistance to integrase strand-transfer inhibitors (InSTIs) was found. Deep sequencing was performed on 48 randomly selected samples and detected additional TDR mutations in 6 cases. Phylogenetic inference showed that 347/403 sequences (86.1%) were part of transmission clusters and identified forward transmission of resistance in Croatia, even that of triple-class resistance. The largest TDR cluster of 53 persons with T215S was estimated to originate in the year 1992. Our data show a continuing need for pre-treatment HIV resistance testing in Croatia. Even though a low prevalence of resistance to InSTI was observed, surveillance of TDR to InSTI should be continued.
Calcium signalling in mammalian cell lines expressing wild type and mutant human α1-Antitrypsin
Authors: Malintan Nancy T. , Buckingham Steven D. , Lomas David A. , Sattelle David B. 
Published: November 2019

AbstractA possible role for calcium signalling in the autosomal dominant form of dementia, familial encephalopathy with neuroserpin inclusion bodies (FENIB), has been proposed, which may point towards a mechanism by which cells could sense and respond to the accumulation of mutant serpin polymers in the endoplasmic reticulum (ER). We therefore explored possible defects in Ca2+-signalling, which may contribute to the pathology associated with another serpinopathy, α1-antitrypsin (AAT) deficiency. Using CHO K1 cell lines stably expressing a wild type human AAT (MAAT) and a disease-causing polymer-forming variant (ZAAT) and the truncated variant (NHK AAT), we measured basal intracellular free Ca2+, its responses to thapsigargin (TG), an ER Ca2+-ATPase blocker, and store-operated Ca2+-entry (SOCE). Our fura2 based Ca2+ measurements detected no differences between these 3 parameters in cell lines expressing MAAT and cell lines expressing ZAAT and NHK AAT mutants. Thus, in our cell-based models of α1-antitrypsin (AAT) deficiency, unlike the case for FENIB, we were unable to detect defects in calcium signalling.
Functional interactions between nitrite reductase and nitric oxide reductase from Paracoccus denitrificans
Authors: Albertsson Ingrid , Sjöholm Johannes , ter Beek Josy , Watmough Nicholas J. , Widengren Jerker , Ädelroth Pia 
Published: November 2019

AbstractDenitrification is a microbial pathway that constitutes an important part of the nitrogen cycle on earth. Denitrifying organisms use nitrate as a terminal electron acceptor and reduce it stepwise to nitrogen gas, a process that produces the toxic nitric oxide (NO) molecule as an intermediate. In this work, we have investigated the possible functional interaction between the enzyme that produces NO; the cd1 nitrite reductase (cd1NiR) and the enzyme that reduces NO; the c-type nitric oxide reductase (cNOR), from the model soil bacterium P. denitrificans. Such an interaction was observed previously between purified components from P. aeruginosa and could help channeling the NO (directly from the site of formation to the side of reduction), in order to protect the cell from this toxic intermediate. We find that electron donation to cNOR is inhibited in the presence of cd1NiR, presumably because cd1NiR binds cNOR at the same location as the electron donor. We further find that the presence of cNOR influences the dimerization of cd1NiR. Overall, although we find no evidence for a high-affinity, constant interaction between the two enzymes, our data supports transient interactions between cd1NiR and cNOR that influence enzymatic properties of cNOR and oligomerization properties of cd1NiR. We speculate that this could be of particular importance in vivo during metabolic switches between aerobic and denitrifying conditions.
Proton-pump inhibitor use is associated with a broad spectrum of neurological adverse events including impaired hearing, vision, and memory
Authors: Makunts Tigran , Alpatty Sama , Lee Kelly C. , Atayee Rabia S. , Abagyan Ruben 
Published: November 2019

AbstractProton-pump inhibitors, PPIs, are considered effective therapy for stomach acid suppression due to their irreversible inhibition of the hydrogen/potassium pump in the gastric parietal cells. They are widely prescribed and are considered safe for over-the-counter use. Recent studies have shown an association between PPI use and Alzheimer dementia, while others have disputed that connection. We analyzed over ten million United States Food and Drug Administration Adverse Event Reporting System reports, including over forty thousand reports containing PPIs, and provided evidence of increased propensity for memory impairment among PPI reports when compared to histamine-2 receptor antagonist control group. Furthermore, we found significant associations of PPI use with a wide range of neurological adverse reactions including, migraine, several peripheral neuropathies, and visual and auditory neurosensory abnormalities.
Sleep timing and duration in indigenous villages with and without electric lighting on Tanna Island, Vanuatu
Authors: Smit Andrea N. , Broesch Tanya , Siegel Jerome M. , Mistlberger Ralph E. 
Published: November 2019

AbstractIt has been hypothesized that sleep in the industrialized world is in chronic deficit, due in part to evening light exposure, which delays sleep onset and truncates sleep depending on morning work or school schedules. If so, societies without electricity may sleep longer. However, recent studies of hunter-gatherers and pastoralists living traditional lifestyles without electricity report short sleep compared to industrialized population norms. To further explore the impact of lifestyles and electrification on sleep, we measured sleep by actigraphy in indigenous Melanesians on Tanna Island, Vanuatu, who live traditional subsistence horticultural lifestyles, in villages either with or without access to electricity. Sleep duration was long and efficiency low in both groups, compared to averages from actigraphy studies of industrialized populations. In villages with electricity, light exposure after sunset was increased, sleep onset was delayed, and nocturnal sleep duration was reduced. These effects were driven primarily by breastfeeding mothers living with electric lighting. Relatively long sleep on Tanna may reflect advantages of an environment in which food access is reliable, climate benign, and predators and significant social conflict absent. Despite exposure to outdoor light throughout the day, an effect of artificial evening light was nonetheless detectable on sleep timing and duration.
Effects of homophily and academic reputation in the nomination and selection of Nobel laureates
Authors: Gallotti Riccardo , De Domenico Manlio 
Published: November 2019

AbstractIn collective decision-making, a group of independent experts propose individual choices to reach a common decision. This is the case of competitive events such as Olympics, international Prizes or grant evaluation, where groups of experts evaluate individual performances to assign resources, e.g. scores, recognitions, or funding. However, there are systems where evaluating individual’s performance is difficult: in those cases, other factors play a relevant role, leading to unexpected emergent phenomena from micro-scale interactions. The Nobel assignment procedure, rooted on recommendations, is one of these systems. Here we unveil its network, reconstructed from official data and metadata about nominators, nominees and awardees between 1901 and 1965, consisting of almost 12,000 individuals and 17,000 nominations. We quantify the role of homophily, academic reputation of nominators and their prestige neighborhood, showing that nominees endorsed by central actors – who are part of the system’s core because of their prestigious reputation – are more likely to become laureate within a finite time scale than nominees endorsed by nominators in the periphery of the network. We propose a mechanistic model which reproduces all the salient observations and allows to design possible countermeasures to mitigate observed effects.
Comparison of Graphene and Carbon Nanotube Saturable Absorbers for Wavelength and Pulse Duration Tunability
Authors: Hussain Syed Asad 
Published: November 2019

AbstractGraphene (Gr) and Carbon nanotube (CNT) saturable absorbers (SAs) are considered as broadband absorbers and have been used in various studies in the past to get a broad wavelength and pulse duration tunable laser. However, the literature lacks a comparative study on these SAs where one will continue to give longer pulses than the other. In this paper we have compared these two SAs and provided a guideline on how to design them for tunable operation. The parameters that have been compared in this research can be used for other broad gain materials and SAs.
Antimicrobial resistance in pathogenic aerobic bacteria causing surgical site infections in Mbarara regional referral hospital, Southwestern Uganda
Authors: Hope Derick , Ampaire Lucas , Oyet Caesar , Muwanguzi Enoch , Twizerimana Hillary , Apecu Richard Onyuthi 
Published: November 2019

AbstractSurgical site infections (SSI) remain a common postoperative complication despite use of prophylactic antibiotics and other preventive measures, mainly due to increasing antimicrobial resistance. Here, we present antimicrobial resistance rate of bacteria isolated in clinical cases of SSI. A hospital based descriptive cross sectional study was conducted on 83 consented postoperative patients with clinical SSI. Data on patients was obtained using structured data collection form. Two swabs were collected aseptically from each patient. Bacteriological culture examination and identification was done following standard microbiological techniques. Antibiotic susceptibility test was done by Kirby-Bauer disc diffusion method. Gram negative bacteria (GNB) were predominant (65.59%) with the dominant being Klebsiella species (29.03%). Overall 86% of aerobic bacteria isolated were multidrug resistant (MDR) where 65.63% and 96.72% of Gram positive and Gram negative isolates were MDR respectively. All the isolates with exception of Enterococci species were resistant to ampicillin. GNB showed high resistance to ceftriaxone, sulfamethoxazole/trimethoprim and gentamicin. All the isolated Klebsiella spp were MDR. S. aureus were all resistant to oxacillin. The isolation rate was higher in emergency, males and dirty wounds in relation to nature of surgery, gender and class of surgical wound respectively. These findings necessitate judicious antibiotic use and calls for surveillance of SSIs periodically as well as strict adherence to good sanitation practice to reduce spread of drug-resistant pathogens.
Understanding the structural degradation of South American historical silk: A Focal Plane Array (FPA) FTIR and multivariate analysis
Authors: Badillo-Sanchez Diego , Chelazzi David , Giorgi Rodorico , Cincinelli Alessandra , Baglioni Piero 
Published: November 2019

AbstractSilk artifacts constitute an invaluable heritage, and to preserve such patrimony it is necessary to correlate the degradation of silk fibroin with the presence of dyes, pollutants, manufacturing techniques, etc. Fourier Transform Infrared Spectroscopy with a Focal plane array detector (FPA FTIR) provides structural information at the micron scale. We characterized the distribution of secondary structures in silk fibers for a large set of South American historical textiles, coupling FTIR with multivariate statistical analysis to correlate the protein structure with the age of the samples and the presence of dyes. We found that the pressure applied during attenuated total reflectance (ATR) measurements might induce structural changes in the fibers, producing similar spectra for pristine and aged samples. Reflectance spectra were thus used for the rigorous characterization of secondary structures. Some correlation was highlighted between the age of the samples (spanning over five centuries) and specific changes in their secondary structure. A correlation was found between the color of the samples and structural alterations, in agreement with the chemical nature of the dyes. Overall, we demonstrated the efficacy of reflectance FPA µ-FTIR, combined with multivariate analysis, for the rigorous and non-invasive description of protein secondary structures on large sets of samples.
Specific heat of 2D interacting Majorana fermions from holography
Authors: Maraner Paolo , Pachos Jiannis K. , Palumbo Giandomenico 
Published: November 2019

AbstractMajorana fermions are a fascinating medium for discovering new phases of matter. However, the standard analytical tools are very limited in probing the non-perturbative aspects of interacting Majoranas in more than one dimensions. Here, we employ the holographic correspondence to determine the specific heat of a two-dimensional interacting gapless Majorana system. To perform our analysis we first describe the interactions in terms of a pseudo-scalar torsion field. We then allow fluctuations in the background curvature thus identifying our model with a (2 + 1)-dimensional Anti-de Sitter (AdS) geometry with torsion. By employing the AdS/CFT correspondence, we show that the interacting model is dual to a (1 + 1)-dimensional conformal field theory (CFT) with central charge that depends on the interaction coupling. This non-perturbative result enables us to determine the effect interactions have in the specific heat of the system at the zero temperature limit.
Antibiotic Resistome Biomarkers associated to the Pelagic Sediments of the Gulfs of Kathiawar Peninsula and Arabian Sea
Authors: Mootapally Chandrashekar , Nathani Neelam M. , Poriya Paresh , Beleem Imtiyaz , Dabhi Jignesh C. , Gadhvi Indra R. , Joshi Chaitanya G. 
Published: November 2019

AbstractAntibiotic resistance has been one of the most persistent global issue. Specifically, marine microbiomes have served as complex reservoirs of antibiotic resistant genes. Molecular advancements have enabled exploration of the uncultured microbial portion from hitherto difficult to sample niches such as deeper oceans. The Gulfs of Kathiawar Peninsula have been known for their unique properties like extreme tidal variations, different sediment textures and physicochemical variations. Pelagic sediment cores across four coordinates each of the Gulf of Kutch, Gulf of Khambhat and an open Arabian Sea were collected, processed for metagenomic sequencing and assessed for antibiotic and metal resistome. The dominant genes were mostly resistant to macrolides, glycopeptides and tetracycline drugs. Studied samples divided into three clusters based on their resistome with carA, macB, bcrA, taeA, srmB, tetA, oleC and sav1866 among the abundant genes. Samples from creek of Gulf of Kutch and mouth of Gulf of Khambhat were most diverse in resistance gene profile. Biomarkers observed include gyrA mutation conferring resistance gene in the Arabian Sea; Proteobacteria species in Gulf of Kutch and Arabian sea; while Aquificae, Acidobacteria and Firmicutes species in the Gulf of Khambhat. Region-wise differentially abundant 23 genes and 3 taxonomic biomarkers were proposed for antibiotic resistance monitoring.
Compton profile of few-layer graphene investigated by electron energy-loss spectroscopy
Authors: Feng Zhenbao , Zhang Xiaoyan , Sakurai Yoshiharu , Wang Zongliang , Li Hefu , Hu Haiquan 
Published: November 2019

AbstractIn this paper, acquisition of the valence Compton profile of few-layer graphene using electron energy-loss spectroscopy at large scattering angle is reported. The experimental Compton profile is compared with the corresponding theoretical profile, calculated using the full-potential linearized augmented plane wave method based on the local-density approximation. Good agreement exists between the theoretical calculation and experiment. The graphene profile indicates a substantially greater delocalization of the ground state charge density compared to that of graphite.
Myc/Max dependent intronic long antisense noncoding RNA, EVA1A-AS, suppresses the expression of Myc/Max dependent anti-proliferating gene EVA1A in a U2 dependent manner
Authors: Niehus Svenja E. , Allister Aldrige B. , Hoffmann Andrea , Wiehlmann Lutz , Tamura Teruko , Tran Doan Duy Hai 
Published: November 2019

AbstractThe Myc gene has been implicated in the pathogenesis of most types of human cancerous tumors. Myc/Max activates large numbers of pro-tumor genes; however it also induces anti-proliferation genes. When anti-proliferation genes are activated by Myc, cancer cells can only survive if they are downregulated. Hepatocellular carcinoma (HCC) specific intronic long noncoding antisense (lnc-AS) RNA, the EVA1A-AS gene, is located within the second intron (I2) of the EVA1A gene (EVA-1 homolog A) that encodes an anti-proliferation factor. Indeed, EVA1A, but not EVA1A-AS, is expressed in normal liver. Depletion of EVA1A-AS suppressed cell proliferation of HepG2 cells by upregulation of EVA1A. Overexpression of EVA1A caused cell death at the G2/M phase via microtubule catastrophe. Furthermore, suppressed EVA1A expression levels are negatively correlated with differentiation grade in 365 primary HCCs, while EVA1A-AS expression levels are positively correlated with patient survival. Notably, both EVA1A and EVA1A-AS were activated by the Myc/Max complex. Eva1A-AS is transcribed in the opposite direction near the 3′splice site of EVA1A I2. The second intron did not splice out in a U2 dependent manner and EVA1A mRNA is not exported. Thus, the Myc/Max dependent anti-proliferating gene, EVA1A, is controlled by Myc/Max dependent anti-sense noncoding RNA for HCC survival.
The Impact of NAFLD and Waist Circumference Changes on Diabetes Development in Prediabetes Subjects
Authors: Lee Jiwoo , Cho Yun Kyung , Kang Yu Mi , Kim Hwi Seung , Jung Chang Hee , Kim Hong-Kyu , Park Joong-Yeol , Lee Woo Je 
Published: November 2019

AbstractThe aim of this study was to investigate the association of nonalcoholic fatty liver disease (NAFLD) with diabetes and the impact of waist circumference (WC) changes in subjects with prediabetes. We enrolled 6240 subjects with prediabetes who underwent health check-ups in 2007 and revisited our hospital at least once for a follow-up examination between 2008 and 2013. Subjects were stratified by WC changes into three groups. The relative risks (RRs) for diabetes according to the NAFLD status and WC change were evaluated. The prevalence of NAFLD was 45.4% (2830/6240). During follow-up, the incidence of diabetes was 8.1% (505/6240). Subjects with NAFLD had a higher incidence of diabetes and the adjusted RRs were 1.81 (95% confidence interval [CI], 1.47 to 2.21), after adjustment for potential confounding factors. The adjusted RRs were related to WC changes. The adjusted RRs for diabetes according to tertiles of WC change (first, second, and third tertile) were 1.64 (95% CI, 1.08 to 2.49), 1.73 (95% CI, 1.28 to 2.34), and 2.04 (95% CI, 1.42 to 2.93), respectively. NAFLD has significantly increased risk of incident diabetes in subjects with prediabetes. The risk for diabetes is gradually increased with tertiles of WC change.