bluuebunny/crossref_metadata_2025_split huggingface dataset:
iTRAQ-based quantitative proteomics analysis of cantaloupe (Cucumis melo var. saccharinus) after cold storage
Authors: Song Wen , Tang Fengxian , Cai Wenchao , Zhang Qin , Zhou Fake , Ning Ming , Tian Huan , Shan Chunhui 
Published: January 2020

Abstract
Background: During the low temperature storage, cantaloupe is susceptible to the cold stress, resulting in the loss of edible and commercial quality. To ascertain the molecular mechanisms of cold tolerance in cantaloupe, cold-sensitive cultivar Goldqueen-308 (GE) and cold-tolerant cultivar Jiashi-310 (JS) were used for quantitative proteomic analysis with iTRAQ in parallel. Results: In this work, two commercial cultivars were treated at 0.5℃ for 0, 12 and 24 days. Phenotypes assays showed that GE suffered a more severe damage as the cold treatment time extended. Proteomic analysis revealed that the number of differentially expressed proteins (DEPs) changed significantly over time in cold-exposed cantaloupe. Comparing with GE, JS responded in a prompter manner in terms of expressing cold-responding proteins during the similarly cold treatment. Furthermore, much more different groups of proteins were mobilized in response to the cold treatment in JS comparing with GE. Metabolic analysis indicated that more amino acids were up-regulated in JS during the early phases of cold stress. This study also identified some DEPs since they were up-regulated in JS or down-regulated in GE in terms of molecular mechanisms, which were mainly related to carbohydrate and energy metabolism, structure proteins, ROS scavening, amino acid metabolic and signaling transduction. Moreover, iTRAQ analysis was confirmed to be reliable via the results of phenotypes assays, metabolic analysis and q-PCR validation. Conclusion: By proteomics information,we found that the prompt response and the significant mobilization of proteins in JS maintained a higher level of cold tolerance, and the delay of cold response in GE could be a critical reason for the severe chilling injury. The candidate proteins we found will be the basis of future studies for further investigations and our findings may help to better understand the novel mechanisms of cold tolerance in cantaloupe.
Protein changes as robust signatures of fish chronic stress: a proteomics approach in fish welfare research
Authors: de Magalhães Cláudia Raposo , Schrama Denise , Farinha Ana Paula , Revets Dominique , Kuehn Annette , Planchon Sébastien , Rodrigues Pedro Miguel , Cerqueira Marco Alexandre 
Published: January 2020

AbstractBackground In the verge of getting a greater understanding of fish welfare, sensitive technological tools, such as proteomics, may assist the aquaculture industry as it allows an unbiased approach for the discovery of potential biomarkers for stress monitoring. Stress is characterized by a cascade of physiological responses that end-up inducing further changes at the whole-animal level that might either increase fitness or impair welfare. Monitorization of this dynamic process, up till now relies on indicators that are only a snapshot of the stress level experienced. Within this scope, using gilthead seabream ( Sparus aurata ) as a model, three chronic stress conditions, namely overcrowding, handling and hypoxia, were employed to evaluate the potential of the fish protein-based adaptations as reliable signatures of chronic stress, in contrast with the commonly used indicators of primary and secondary stress responses.Results A large spectrum of biological variation regarding cortisol and glucose levels was observed, which values rose higher in net handled fish. In this sense, a potential pattern of stressor-specificity was evidenced since the magnitude of response and tolerance varied markedly from a permanent (crowding) to a repetitive stressor (handling). Gel-based proteomics analysis of the plasma proteome also revealed that net handled fish had the highest number of proteins with significantly altered abundance, compared to the other trials, whereas mass spectrometric analysis, followed by gene ontology enrichment and protein-protein interaction analyses, characterized those as humoral components of the innate immune system and key elements on the response to stimulus.Conclusions Overall, this study represents the first screening of more reliable signatures of physiological adaptation to chronic stress in fish, allowing the future development of novel biomarker models to monitor fish welfare.
On the utility of RNA sample pooling to optimize cost and statistical power in RNA sequencing experiments
Authors: Assefa Alemu Takele , Vandesompele Jo , Thas Olivier 
Published: January 2020

Abstract
In gene expression studies, RNA sample pooling is sometimes considered because of budget constraints or lack of sufficient input material. Using microarray technology, RNA sample pooling strategies have been reported to optimize both the cost of data generation as well as the statistical power for differential gene expression (DGE) analysis. For RNA sequencing, with its different quantitative output in terms of counts and tunable dynamic range, the adequacy and empirical validation of RNA sample pooling strategies have not yet been evaluated. In this study, we comprehensively assessed the utility of pooling strategies in RNA-seq experiments using empirical and simulated data. Mathematical descriptions of the data generating mechanism in pooled experiments are used to reinforce our interpretations from the empirical and simulation studies. The results demonstrate that pooling strategies in RNA-seq studies can be both cost-effective and powerful when the number of pools, pool size and sequencing depth are optimally defined. For high within-group gene expression variability, small RNA sample pools are effective to reduce the variability and compensate for the loss of the number of replicates. Unlike the typical cost-saving strategies, such as reducing sequencing depth or number of biological samples, an adequate pooling strategy is effective in maintaining the power of testing for DGE for low to medium abundance levels, along with a substantial reduction of the total cost of the experiment.
EMG-pressure Response in the Transverse Abdominis and Multifidus Muscles among Individuals With and Without Chronic Low Back Pain
Authors: Wang Chu huai , Li Xin , Lo Wai Leung Ambrose , Lu Song wei , Liu Howe , Lin Ke yu , Lai Jian yang , Li Le 
Published: January 2020

Abstract
Background: Pressure biofeedback unit (PBU) is a non-invasive, low-cost, and widely used device for monitoring, evaluating and training transverse abdominals (TA) and multifidus (MF) muscles of patients with low back pain (LBP). There has been little research on quantifying muscle activity under different pressures. The primary aim of this preliminary study was to explore the response between deep local trunk muscles (TA and MF) and different target pressures of PBU in seated positions. Methods: Twenty-two patients with chronic LBP (cLBP) and 24 age matched healthy individuals were recruited. Electromyography (EMG) signals were recorded from the TA and MF muscles while individuals contract the TA and MF muscles in seated position to achieve PBU pressure value of 50, 60 and 70mmHg in random order. The t-test was used to compare between-group and within-subjects’ effects to examine the effect of different pressure values. Spearman’s correlation analysis was performed in the cLBP group to determine potential correlations. Results: The %MVIC of the TA and MF in the cLBP group were statistically higher than the control group at each pressure value (P＜0.05). The slope of the cLBP subjects was significantly steeper than the healthy control subjects (TA: P=0.01, MF: P＜0.001). During maximal voluntary isometric contraction (MVIC) of TA and MF, compared with pain-free group, cLBP patients showed a significant decrease (P≤0.001). MF MVIC was significantly and moderately negatively correlated with visual analog scale (VAS) (r = -0.48, P=0.024) and Oswestry Disability Index (ODI) (r = -0.59, P=0.004). Conclusions: The study demonstrates the feasibility of using PBU to assess muscle contraction that corresponds with changes of muscle activity as measured by EMG. The use of EMG to quantify the extent of how much the PBU activates muscles may provide important information to clinicians and researchers for patients with LBP.
Network Pharmacology Analysis on Zhichan Powder in the Treatment of Parkinson's Disease
Authors: Li Jia , Qi Xinchang , Sun Yajuan , Zhang Yingyu , Chen Jiajun 
Published: January 2020

Aim and Objective:
Effective components and the mechanism of action of Zhichan powder
for the treatment of Parkinson's disease were researched at a systematic level.

Materials and Methods:
Screening of active components in Zhichan powder for the treatment of
Parkinson's disease was conducted using the Traditional Chinese Medicine Systems Pharmacology
database, and a medicine-target-disease network was established with computational network
pharmacology.

Results:
By using network pharmacology methods, we identified 18 major active components in
Zhichan powder through screening, indicating a connection between chemical components of this
Traditional Chinese Medicine and Parkinson’s disease-related targets.

Conclusion:
The medicine-target-disease system of Zhichan powder established by network
pharmacology permitted visualization of clustering and differences among chemical components in
this prescription, as well as the complex mechanism of molecular activities among those effective
components, relevant targets, pathways, and the disease. Thus, our results provide a new perspective
and method for revealing the mechanism of action of Traditional Chinese Medicine prescriptions.
Therapeutic Study of Phytochemicals Against Cancer and Alzheimer’s Disease Management
Authors: Ahmad Syed Sayeed , Waheed Tayyaba , Rozeen Sayed , Mahmood Sufia , Kamal Mohammad Amjad 
Published: January 2020

Background:Phytochemicals are a significant piece of conventional prescription and have been researched in detail for conceivable consideration in current drug discovery. Medications and plants are firmly identified for traditional prescriptions and ethnomedicines that are basically arranged from plants. Recognizing the medical advantages of phytochemicals is of fundamental advancement in medication and useful sustenance improvement. Secondary metabolites of different plants have been customarily used for the improvement of human wellbeing. The phytochemicals are diets rich, which can upgrade neuroplasticity and protection from neurodegeneration.Results:Phytochemicals keep on entering clinical preliminaries or provide leads for the synthesis of medicinal agents. Phytochemicals are a great extent cancer prevention agents in nature at lower concentrations and under favorable cell conditions that adequately avoid the oxidation of different molecules that have an ability to produce free radicals and thus protect the body.Conclusion:The purpose of this review is to describe the use of phytochemicals against cancer and Alzheimer’s disease treatment.
Hydroxycitric Acid Inhibits Renal Calcium Oxalate Deposition by Reducing Oxidative Stress and Inflammation
Authors: Liu Xiao , Yuan Peng , Sun Xifeng , Chen Zhiqiang 
Published: January 2020

Objective:
The study aimed to evaluate the preventive effects of hydroxycitric
acid（HCA） for stone formation in the glyoxylate-induced mouse model.

Materials and Methods:
Male C57BL/6J mice were divided into a control group,
glyoxylate(GOX) 100 mg/kg group, a GOX+HCA 100 mg/kg group, and a GOX+HCA
200 mg/kg group. Blood samples and kidney samples were collected on the eighth day
of the experiment. We used Pizzolato staining and a polarized light microscope to
examine crystal formation and evaluated oxidative stress via the levels of
malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione peroxidase
(GSH-Px). Quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) was
used to detect the expression of monocyte chemotactic protein-1(MCP-1), nuclear
factor-kappa B (NF κ B), interleukin-1 β (IL-1 β) and interleukin-6 (IL-6) messenger RNA
(mRNA). The expression of osteopontin (OPN) and a cluster of differentiation-44(CD44)
were detected by immunohistochemistry and qRT-PCR. In addition, periodic acid Schiff
(PAS) staining and TUNEL assay were used to evaluate renal tubular injury and
apoptosis.

Results:
HCA treatment could reduce markers of renal impairment (Blood Urea
Nitrogen and serum creatinine). There was significantly less calcium oxalate crystal
deposition in mice treated with HCA. Calcium oxalate crystals induced the production of
reactive oxygen species and reduced the activity of antioxidant defense enzymes. HCA
attenuated oxidative stress induced by calcium oxalate crystallization. HCA had
inhibitory effects on calcium oxalate-induced inflammatory cytokines, such as MCP-1, IL-
1 β, and IL-6. In addition, HCA alleviated tubular injury and apoptosis caused by calcium
oxalate crystals.

Conclusion:
HCA inhibits renal injury and calcium oxalate crystal deposition in the
glyoxylate-induced mouse model through antioxidation and anti-inflammation.
Upper and Lower Airways Diseases in Childhood
Authors: Parisi Giuseppe Fabio , Leonardi Salvatore 
Published: January 2020

:
In the last decade, extensive research has provided consistent advances in molecular biology and “omics”
science (genomics, metabolomics, proteomics, and transcriptomics), which have led to the detailed characterization of the
etiology, pathophysiological mechanisms, and subtypes of many diseases. Based on these developments, the concept of
precision medicine was established.

:
About a quarter of medical consultations related to primary care in children involves respiratory problems. About 10% of
the cases are asthma, while the other most important respiratory diseases includes bronchiolitis, acute bronchitis and
respiratory infections. Today, beyond the most common respiratory pathology, it is appropriate to discuss what are the
novelties in the management of more complex, frequent and infrequent, pathologies because they are those in which it is
possible to implement the concept of precision medicine.
Evaluation of Mandibular Third Molar Region in a Turkish Population Using Cone-Beam Computed Tomography
Authors: Orhan Büyük Kaan , Yılmaz Dilek , Ozemre Mehmet Ozgur , Kamburoğlu Kıvanç , Gulen Orhan , Gulsahi Ayse 
Published: January 2020

Objectives:
To evaluate impacted mandibular third molar tooth region and obtain linear
measurements using CBCT images and to assess the relationship between the impacted third molar
and the mandibular canal.

Methods:
CBCT scans of 351 patients (208 females, 143 males) were assessed. Age, gender, and
impaction site were recorded for each patient. The relationship of third molars with the vertical
axis of second molars, 2nd molar resorption and the relationship between third molar apices and
the mandibular canal were assessed. In addition, the distance between ramus and second molar,
mesiodistal width of the third molar, the angle between third molar and second molar, and width
of the third molar capsule were measured. Binary Logistic Regression, Chi-Square Test, and General
Linear Model were used for statistical analysis.

Results:
The highest percentage of impaction was found for mesioangular followed by transversal
and vertical. The transversal impacted third molars revealed a significant association with adjacent
second molar root resorption (p<0.001). There was a statistical significance between the second
molar resorption and distance between ramus and second molar (p<0.001). The mesioangular impacted
third molars revealed significant relation with the mandibular canal (p<0.05). The most
frequent variation found was the dental canal followed by the retromolar canal. In general, higher
measurement values were obtained for men when compared to women (p<0.05).

Conclusion:
CBCT assessment of the third molar region provided useful information regarding
impacted mandibular third molar surgery operations.
PELATIHAN BATIK CELUP DI GAMPONG JANTHO
Authors: Naufa Miftahun , Andeska Niko , Kholilah Anni 
Published: January 2020

AbstrakJantho merupakan sebuah daerah dengan dataran tinggi yang menjadi ibu kota Aceh Besar, kota Jantho menjadi pusat kota dan menjadi pusat pemerintahan serta administrasi untuk daerah Aceh Besar, meskipun kota Jantho menjadi pusat administrasi, tetapi kota Jantho masih dominan dengan daerah perhutanan dan perbukitan. Gampong Jantho memiliki sumber daya alam yang besar namun belum dapat termanfaatkan secara maksimal oleh masyarakat dan pemerintah Aceh Besar. Melihat sumber daya alam yang besar menjadi ide dan daya tarik untuk mengolahnya sehingga dapat meningkatkan perekonomian daerah tersebut. Permasalahan yang ada di Gampong Jantho adalah kurangnya pembinaan dalam sektor pembuatan kerajinan. Dengan terselenggaranya pengabdian masyarakat diharapkan dapat menambah dan mengembangkan wawasan masyarakat untuk membuat karya yang memiliki nilai jual. Salah satu upaya yang ingin dikembangkan adalah membuat kerajinan batik celup, batik celup merupakan satu kerajinan batik yang sangat mudah dipelajari dan dapat dimanfaatkan untuk meningkatkan sektor perdagangan masyarakat setempat.Kata Kunci: batik celup, gampong jantho.AbstractJantho is a highland area which is the capital of Aceh Besar, Jantho city is the center of the city and the administrative and administrative center for the Greater Aceh area, although Jantho city is the administrative center, but Jantho city is still dominant in forest and hilly areas. Gantong Jantho has large natural resources but cannot be fully utilized by the community and the government of Aceh Besar. Seeing large natural resources is an idea and attraction to process them so that they can improve the economy of the area. The problem that exists in Jantho Village is the lack of guidance in the craft making sector. With the implementation of community service, it is expected to be able to add and develop community insight to create works that have a sale value. One of the efforts to be developed is to make dyed batik, batik dye is a batik craft that is very easy to learn and can be used to improve the local community's trade sector.Keywords: batik dyes, gampong jantho.
KEBERADAAN RAPA’I DABO’IH GRUP BUNGONG SITANGKEE SEBAGAI REPRODUKSI BUDAYA DI PERKAMPUNGAN BEKAS EVAKUAS CARE KORBAN PASCA TSUNAMI ACEH
Authors: Andika Beni , Sari Fani Dila 
Published: January 2020

AbstrakKreativitas grup Bungong Sitangkee mempertunjukan Rapa’i Daboi’h merupakan upaya reproduksi budaya di perkampungan bekas pengungsian NGO (Non Goverment Organization) CARE di perkampungan Teurebeuh Kecamatan Kota Jantho Kabupaten Aceh Besar. Rapa’i Dabo’ih adalah seni pertunjukan atraksi yang menakjubkan. Seni pertunjukan ini digemari karena bentuk sajian pertunjukan Rapa’i Dabo’ih yang atraktif dengan debus sebagai puncak dari permainan yang disertai instrumen Rapa’i dengan lantunan syair-syair berisikan syiar agama Islam. Reproduksi budaya adalah proses mempertahankan identitas budaya yang dilakukan oleh masyarakat korban pascatsunami yang sudah tinggal menetap di area bekas pengungsian sebagai pelestarian dan eksistensi kebudayaan asalnya. Tujuan penelitian ini adalah mengungkap keberadaan Rapa’i Daboih sebagai reproduksi budaya yang terjadi di perkampungan CARE bekas pengungsian korban pascatsunami di Aceh 2004 lalu. Identifikasi reproduksi budaya ditinjau dari pemkanaan ulang seni pertunjukan Rapa’i Dabo’ih oleh Grup Bungong Sitangkee di Kecamatan Kota Jantho Kabupaten Aceh Besar. Metode yang digunakan adalah metode kualitatif, pengumpulan data dilakukan melalui observasi dan mengamati pertunjukan dan wawancara. Hasil penelitian ini mengungkapkan bagaimana kebradaan reproduksi budaya dengan studi kasus Rapa’i Dabo’ih.Kata Kunci: rapa’i dabo’ih, reproduksi, budaya.AbstractThe Bungong Sitangkee group's creativity demonstrates Rapa 'i Daboi'h is an effort to reproduce culture in the CARE (Non Government Organization) in Teurebeuh village, JAntho City District, Aceh Besar District. Rapa’i Dabo’ih is an amazing performing arts performance. This performance art is favored because of the attractive form of the Rapa'i Dabo'ih show with debus as the culmination of the game accompanied by the Rapa'i instrument with the recitation of verses containing Islamic symbols. Cultural reproduction is the process of maintaining cultural identity carried out by post-tsunami victims who have settled in ex-refugee areas as a preservation and existence of their original culture. The purpose of this study is to uncover the existence of Rapa'i Daboih as a cultural reproduction that occurred in the CARE village of ex-refugee victims after the tsunami in Aceh in 2004. Identification of cultural reproduction is reviewed from the reopening of the Rapa 'i Dabo'ih performance by the Bungong Sitangkee Group in Kota Jantho District, Aceh Besar Regency. The method used is a qualitative method, data collection is done through observation and observing performances and interviews. The results of this study reveal how the existence of cultural reproduction with the Rapa'i Dabo'ih case study. Keywords: rapa'i dabo’ih, reproduction, culture.
English Self-Assessment Based on Pluralism in Preventing Radicalism
Authors: Andriyani Santi , Yulistianti Hayu Diah , Saidah Nusrotus 
Published: January 2020

The practice of radicalism in Indonesia has increased among all age groups, including young generation. They were mostly affected by radicalism and extremism. The aim of this study was to find out the implementation of English Self-Assessment Based on Pluralism in Preventing Youth Radicalism. This study used a descriptive analysis method. The instrument of this study was the students’ self-assessment worksheet focused on pluralism materials. The result showed that the implementation of English self-assessment through the values ​​of pluralism can improve the students’ understanding by using the technique of reading comprehension. And the religious understanding of the students was good criteria
Keywords: English Self-Assessment, Preventing Radicalism, Self-Assessment
ANALISIS ANGGARAN PARSIAL DAN PREFERENSI PETANI TERHADAP KARAKTER BEBERAPA VARIETAS UNGGUL PADI LAHAN SAWAH TADAH HUJAN DI KABUPATEN SAMBAS (STUDI KASUS DESA MATANG DANAU, KEC.PALOH)
Authors: Burhansyah Rusli 
Published: January 2020

Problems with rainfed lowland rice cultivation in Sambas Regency include; the level of productivity is still low and the attack of blast disease and planthopper pests. Therefore, the introduction of new high-yielding varieties that have high productivity and resistant to blast disease and planthopper pests. Besides that, new improved varieties are in accordance with the tastes of the local community. This study aims to determine the analysis of the financial feasibility and preferences of farmers to the character of superior varieties of rice in rain-fed rice fields. The field research was carried out on 10 hectares of farmers' land and survey in Matang Danau village, Paloh sub-district. Research time is from April to August 2018. Respondents were 30 ethnic Malay cooperative farmers. Data collected included input and output data of rice farming, farmer characteristics, plant morphology. Data collection through observation and interviews with farmers. Observations were made on growth in the field and just before harvest. From the results of the study showed that the new superior variety of Inpari 41 was feasible to be cultivated with MBCR 1.49 with additional gross revenue of Rp 5,435,000 (39.92%) with again on the total cost of Rp 1,252,940 (22.45%) of the variety Cilosari. Farmers like the Inpari 41 variety because based on plant type, plant height, panicle length, number of productive tillers, level of production, resistance to pests and diseases, grain loss rate, grain color, aspects that get attention in developing rice in rainfed lowland are vegetative growth, grain form, quality of rice, rice texture, and taste of rice that are favored by local farmers as information for breeders in assembling new superior varieties of rainfed lowland rice. Keywords: Financial feasibility, Preference, New high yielding varieties, Rainfed lowland