bluuebunny/crossref_metadata_2025_split huggingface dataset:
The prophylactic and therapeutic effects of moxibustion combined with traditional Chinese medicine decoction for treating chemotherapy-induced myelosuppression in early-stage breast cancer: study protocol for a randomized controlled trial
Authors: Ji Yajie , Li Siyu , Zhang Xinyue , Liu Yu , Lu Qing , Li Qiong , Chen Weili , Sheng Jiayu , Jiang Ke , Liang Hongli , Sha Shanyan , Li Mengting , Chen Zongxin , Zheng Peiyi , Wang Minhong , Feng Yuanyuan , Wang Lei , Wu Huangan , Liu Huirong , Huang Yan , Yin Zhiguang , Xue Xiaohong 
Published: October 2020

Abstract
Background
Traditional Chinese medicine (TCM) has a long history of use in breast cancer, but lacking systematic evidence to support its clinical benefits. In this study, we evaluated the prophylactic and therapeutic effects of moxibustion combined with decoctions for treating chemotherapy-induced myelosuppression (CIM) in early-stage breast cancer patients.

Methods
This is a randomized controlled clinical trial single-blinded for TCM decoction but not moxibustion. Patients are equally divided into the control group without decoction and moxibustion treatment (control), the decoction+moxibustion group (MD), and the placebo+moxibustion group (MP), according to the following stratification factors: age (below 40s, 40s, 50s, and 60s or above), chemotherapy regimen (anthracyclines, taxanes, anthracyclines+taxane, and others), and chemotherapy strategy (adjuvant and neoadjuvant). The TCM decoction is Wenshen Shengbai Decoction. The anticipated sample size is 462 cases (154 cases in each group). All participants are expected to treat with chemotherapy and recombinant human granulocyte colony-stimulating factor (rhG-CSF). The primary outcomes include the proportion of patients with relief of leukopenia and/or neutropenia, the myelosuppression-associated serious adverse event including grade 3–4 leukopenia and/or neutropenia, and febrile neutropenia, and the dose of rhG-CSF. The secondary outcomes include chemotherapy adherence, stratified analysis, adverse reactions, quality of life by EORTC Breast-Cancer-Specific Quality of Life Questionnaire including EORTC QLQ-C30 (V3.0) and QLQ-BR23, TCM Constitution, and 3-year disease-free survival and overall survival. Baseline information including age, surgical approach, chemotherapy regimen and strategy, pathological stage, and molecular subtype will be recorded.

Discussion
This will be the first randomized controlled trial to evaluate the efficacy of moxibustion combined with TCM decoction in treating CIM in early-stage breast cancer patients, aiming to standardize the TCM decoction and moxibustion method, thus providing evidence for its clinical benefit.

Trial registration
chictr.org.cn ChiCTR-INR-16009557. Registered on 23 October 2016.
Epigenomic landscape of enhancer elements during Hydra head organizer formation
Authors: Reddy Puli Chandramouli , Gungi Akhila , Ubhe Suyog , Galande Sanjeev 
Published: October 2020

Abstract
Background
Axis patterning during development is accompanied by large-scale gene expression changes. These are brought about by changes in the histone modifications leading to dynamic alterations in chromatin architecture. The cis regulatory DNA elements also play an important role towards modulating gene expression in a context-dependent manner. Hydra belongs to the phylum Cnidaria where the first asymmetry in the body plan was observed and the oral-aboral axis originated. Wnt signaling has been shown to determine the head organizer function in the basal metazoan Hydra.

Results
To gain insights into the evolution of cis regulatory elements and associated chromatin signatures, we ectopically activated the Wnt signaling pathway in Hydra and monitored the genome-wide alterations in key histone modifications. Motif analysis of putative intergenic enhancer elements from Hydra revealed the conservation of bilaterian cis regulatory elements that play critical roles in development. Differentially regulated enhancer elements were identified upon ectopic activation of Wnt signaling and found to regulate many head organizer specific genes. Enhancer activity of many of the identified cis regulatory elements was confirmed by luciferase reporter assay. Quantitative chromatin immunoprecipitation analysis upon activation of Wnt signaling further confirmed the enrichment of H3K27ac on the enhancer elements of Hv_Wnt5a, Hv_Wnt11 and head organizer genes Hv_Bra1, CnGsc and Hv_Pitx1. Additionally, perturbation of the putative H3K27me3 eraser activity using a specific inhibitor affected the ectopic activation of Wnt signaling indicating the importance of the dynamic changes in the H3K27 modifications towards regulation of the genes involved in the head organizer activity.

Conclusions
The activation-associated histone marks H3K4me3, H3K27ac and H3K9ac mark chromatin in a similar manner as seen in bilaterians. We identified intergenic cis regulatory elements which harbor sites for key transcription factors involved in developmental processes. Differentially regulated enhancers exhibited motifs for many zinc-finger, T-box and ETS related TFs whose homologs have a head specific expression in Hydra and could be a part of the pioneer TF network in the patterning of the head. The ability to differentially modify the H3K27 residue is critical for the patterning of Hydra axis revealing a dynamic acetylation/methylation switch to regulate gene expression and chromatin architecture.
Chemotherapy combined with radiotherapy for successful treatment of angioimmunoblastic T-cell lymphoma: a case report
Authors: Xiong Yudi , Yang Lei , Dai Jing , Zhou Fuxiang , Zhou Yunfeng 
Published: October 2020

Abstract
Background
The incidence of angioimmunoblastic T-cell lymphoma is rare worldwide, and it has a poor prognosis. There is no proven or standard first-line therapy that works for the majority of patients with angioimmunoblastic T-cell lymphoma because of the rarity of this disease. The treatment and management are challenging for clinicians.

Case presentation
This report presents the diagnosis and treatment of a 65-year-old Chinese man who presented with cough and lymph node swellings in the left axillary region. The patient was diagnosed with angioimmunoblastic T-cell lymphoma. He underwent eight cycles of chemotherapy with CHOP (cyclophosphamide, hydroxydaunorubicin, oncovin, prednisone) followed by TOMO radiotherapy (helical tomotherapy, a kind of radiotherapy for cancer treatment using spiral computed tomographic scanning). After treatment, the therapeutic effects were evaluated by magnetic resonance imaging and computed tomography about every 3 months. The patient recovered well with no sign of tumor recurrence and no obvious severe treatment-related adverse effects.

Conclusion
This treatment experience indicates an essential role for the combination of radiation therapy with CHOP, which may have a better prognosis than treatments without radiation therapy. But challenges warrant further validation in prospective studies.
Advances in the role of m6A RNA modification in cancer metabolic reprogramming
Authors: Han Xiu , Wang Lin , Han Qingzhen 
Published: October 2020

AbstractN6-methyladenosine (m6A) modification is the most common internal modification of eukaryotic mRNA and is widely involved in many cellular processes, such as RNA transcription, splicing, nuclear transport, degradation, and translation. m6A has been shown to plays important roles in the initiation and progression of various cancers. The altered metabolic programming of cancer cells promotes their cell-autonomous proliferation and survival, leading to an indispensable hallmark of cancers. Accumulating evidence has demonstrated that this epigenetic modification exerts extensive effects on the cancer metabolic network by either directly regulating the expression of metabolic genes or modulating metabolism-associated signaling pathways. In this review, we summarized the regulatory mechanisms and biological functions of m6A and its role in cancer metabolic reprogramming.
Revealing historical fire regimes of the Cumberland Plateau, USA, through remnant fire-scarred shortleaf pines (Pinus echinata Mill.)
Authors: Stambaugh Michael C. , Marschall Joseph M. , Abadir Erin R. 
Published: October 2020

Abstract
Background
Vegetation of the Cumberland Plateau (USA) has undergone dramatic transitions since the last glaciation and particularly since the onset of widespread logging and twentieth century fire exclusion. Shortleaf pine (Pinus echinata Mill.), one of the most fire-dependent conifers in the US, occurs throughout the Cumberland Plateau, but its abundance has declined dramatically since Euro-American settlement and continues to decline. To better understand the historical ecology of fire within the natural range of shortleaf pine, we reconstructed fire regimes at three new sites throughout the central and southern Cumberland Plateau region based on fire scars on shortleaf pine trees.

Results
Fire event chronologies extended back to the seventeenth century and revealed historical fire regimes that were frequent and dominated by dormant-season and low-severity events. Fires occurred on average every 4.4 to 5.3 years at the study sites before widespread Euro-American settlement, and were more frequent (2.3 to 3.8 years) following settlement. Cumberland Plateau fires may be linked to adjacent ecoregions such as the Eastern Highland Rim to the west. Among all sites, we found that long-term trends in fire activity were similar and fit into a regional waveform pattern of fire activity likely driven by humans (i.e., Native American depopulation, European settlement, and twentieth century fire exclusion).

Conclusions
The decline in shortleaf pine and other fire-dependent ecosystems across the Cumberland Plateau is due to multiple interacting factors and, based on these data, frequent fire should be considered a historically important ecological driver of these systems.
Real-time integrity monitoring for a wide area precise positioning system
Authors: Wang Yuechen , Shen Jun 
Published: October 2020

AbstractThe wide area precise positioning system (WAPPS) is a high-precision positioning system based on a global navigation satellite system. Using a GEO satellite or a communication network, it provides users, in its service area, with real-time satellite orbit, clock, and other corrections. Users can achieve centimeter-level static positioning or decimeter-level kinematic positioning by precise point positioning. With the demands for applications of both high-precision and safety of life in real time, WAPPS is facing urgent needs to improve its service integrity. This study presents a real-time integrity monitoring approach for WAPPS. Using dual-frequency ionosphere-free corrections of GPS and BDS, along with monitor station data, related error models are established and the integrity monitoring is achieved, based on the analysis of satellite corrected residuals. In addition, satellite faults are simulated for performance verification. The results show that the algorithm can monitor both step and drift faults effectively and alert users in time.
Loss of Inhibin Advances Follicle Activation and Female Puberty Onset but Blocks Oocyte Maturation in Zebrafish
Authors: Lu Huijie , Zhao Cheng , Zhu Bo , Zhang Zhiwei , Ge Wei 
Published: October 2020

Abstract
Inhibin was first characterized in mammals as a gonadal dimeric protein that inhibited pituitary follicle-stimulating hormone (FSH) secretion. As in mammals, the inhibin-specific α subunit (INHA/Inha/inha) has also been characterized in teleosts; however, its functions and physiological importance in fish reproduction remain unknown. Using CRISPR/Cas9 method, we generated an inha-deficient zebrafish line and analyzed its reproductive performance. As expected, pituitary expression of fshb increased significantly in both the young and the adult inha mutant. The expression of lhb also increased in the mutant, but only in sexually mature adults. Interestingly, the expression of activin βA (inhbaa) increased significantly in both the ovary and the testis of inha mutant, and the expression of ovarian aromatase (cyp19a1a) also increased dramatically in the mutant ovary. The juvenile female mutant showed clear signs of early follicle activation or precocious puberty onset. However, the adult female mutant was infertile with follicles arrested at the full-grown stage without final oocyte maturation and ovulation. Although follicle growth was normal overall in the mutant, the size and distribution of yolk granules in oocytes were distinct and some follicles showed granulosa cell hypertrophy. In contrast to females, inha-null males showed normal spermatogenesis and fertility. As reported in mammals, we also found sporadic tumor formation in inha mutants. Taken together, our study not only confirmed some conserved roles of inhibin across vertebrates, such as inhibition of FSH biosynthesis and tumor formation, but also revealed novel aspects of inhibin functions such as disruption of folliculogenesis and female infertility but no obvious involvement in spermatogenesis in fish.
Assembling the Laity: Standardizing Lay Buddhist Affiliation via Education in Contemporary Korea
Authors: Kaplan Uri 
Published: October 2020

AbstractThis paper discusses the new system of lay Buddhist education revolutionizing the Chogye Order of Korean Buddhism in the twenty-first century. Already involving over a half a million Buddhist laypeople and five hundred monasteries throughout the country, this system is now the hallmark of the Chogye Order’s propagation agenda, and it has transformed lay Buddhist activities in contemporary Korea. It is grounded upon a novel lay registration network which requires attending a basic Buddhism course at a designated temple in order to receive an official Lay ID. It now involves a lay ranking structure based on educational achievements, and culminates in the yearly Lay Propagator Exam. Besides delineating the historical construction of this system, this paper will investigate lay attitudes and motivations for joining the programs, analyze official textbook agendas, and provide ethnographic snippets from classroom rituals, in an overall attempt to paint a picture of the new Korean Buddhist lay orthodoxy the Chogye Order is currently in the process of creating.
Cold War Mnemonics: History, Melancholy, and Landscape in South Korean Films of the 1960s
Authors: Park Hyun Seon 
Published: October 2020

AbstractThis article examines the way that cinematic mnemonics of 1960s South Korean films ciphers the heterogeneous and conflicting experiences regarding two entangled wars: the Korean War and the Cold War. In a close reading of Kim Suyong’s Mist (An’gae, 1967) and Yi Sŏnggu’s The General’s Mustache (Changgun ŭi suyŏm, 1968), the article argues for the multifaceted aesthetics of Cold War mnemonics, which illuminates a binding and unbinding technology of affective memories in which the traumatic experience of the Korean war parallels the dominant narrative of Cold War historiography. In Mist and The General’s Mustache, historical trauma and the experience of loss take up important positions in relation to melancholic landscape and mnemonic devices. Visualizing the interstice between melancholy and mourning, between memory and history, and between landscape and interiority through the devices of flashback, widescreen, montage, and metanarrative structure, the exploration of mnemonic technologies is inextricably linked with the postwar Korean subject’s dual efforts to remember historical loss and to incorporate shameful memories. While Mist shows the male protagonist’s short visit to his countryside hometown, during which he is troubled by memories of the past and, thus, his encounter with the unfinished work of mourning, The General’s Mustache, beginning with a photojournalist’s suspicious death, assembles the fragmentary pieces of modern Korean history’s secrets through multiple frames of testimony and confession. Produced during the time of Cold War turmoil as well as at the height of global modernization, these films release alternative thinking about time, memory, and history, asking us to remember what is left behind in Cold War historiography.
Departure and Repatriation as Cold War Dissensus: Domestic Ethnography in Korean Documentary
Authors: Park Jinhee 
Published: October 2020

AbstractThis article examines autobiographic documentaries about families that expose “dissensus” in the mapping of transborder migration and diasporic desire that were the results of the Cold War in North Korea, South Korea, and Japan. Jae-hee Hong (dir. My Father’s Emails) and Yong-hi Yang (dir. Dear Pyongyang and Goodbye Pyongyang) document the ongoing Cold War in their fathers’ histories through their position as a “familial other,” who embodies both dissensus and intimacy. Hong reveals that anticommunism in South Korean postwar nation building reverberated in the private realm. Yang documents her Zainichi father, who sent his sons to North Korea during the Repatriation Campaign in Japan. The anticommunist father in South Korea (Hong’s) and the communist father in Japan (Yang’s) engendered family migration with contrasting motivations, departure from and return to North Korea, respectively. Juxtaposing these two opposite ideologies in family histories, as well as juxtaposing the filmmakers’ dissonance with the given ideologies in domestic space, provide the aesthetic form for “dissensus.” The politics of aesthetics in domestic ethnography manifests in that the self and the Other are inextricably interlocked because of the reciprocity of the filmmaker and the communist or anticommunist subject.
CHIP mutations affect the heat shock response differently in human fibroblasts and iPSC-derived neurons
Authors: Schuster S. , Heuten E. , Velic A. , Admard J. , Synofzik M. , Ossowski S. , Macek B. , Hauser S. , Schöls L. 
Published: October 2020

ABSTRACT
C-terminus of HSC70-interacting protein (CHIP) encoded by the gene STUB1 is a co-chaperone and E3 ligase that acts as a key regulator of cellular protein homeostasis. Mutations in STUB1 cause autosomal recessive spinocerebellar ataxia type 16 (SCAR16) with widespread neurodegeneration manifesting as spastic-ataxic gait disorder, dementia and epilepsy. CHIP−/− mice display severe cerebellar atrophy, show high perinatal lethality and impaired heat stress tolerance. To decipher the pathomechanism underlying SCAR16, we investigated the heat shock response (HSR) in primary fibroblasts of three SCAR16 patients. We found impaired HSR induction and recovery compared to healthy controls. HSPA1A/B transcript levels (coding for HSP70) were reduced upon heat shock but HSP70 remained higher upon recovery in patient- compared to control-fibroblasts. As SCAR16 primarily affects the central nervous system we next investigated the HSR in cortical neurons (CNs) derived from induced pluripotent stem cells of SCAR16 patients. We found CNs of patients and controls to be surprisingly resistant to heat stress with high basal levels of HSP70 compared to fibroblasts. Although heat stress resulted in strong transcript level increases of many HSPs, this did not translate into higher HSP70 protein levels upon heat shock, independent of STUB1 mutations. Furthermore, STUB1(−/−) neurons generated by CRISPR/Cas9-mediated genome editing from an isogenic healthy control line showed a similar HSR to patients. Proteomic analysis of CNs showed dysfunctional protein (re)folding and higher basal oxidative stress levels in patients. Our results question the role of impaired HSR in SCAR16 neuropathology and highlight the need for careful selection of proper cell types for modeling human diseases.
The F1 loop of the talin head domain acts as a gatekeeper in integrin activation and clustering
Authors: Kukkurainen Sampo , Azizi Latifeh , Zhang Pingfeng , Jacquier Marie-Claude , Baikoghli Mo , von Essen Magdaléna , Tuukkanen Anne , Laitaoja Mikko , Liu Xiaonan , Rahikainen Rolle , Orłowski Adam , Jänis Janne , Määttä Juha A. E. , Varjosalo Markku , Vattulainen Ilpo , Róg Tomasz , Svergun Dmitri , Cheng R. Holland , Wu Jinhua , Hytönen Vesa P. , Wehrle-Haller Bernhard 
Published: October 2020

ABSTRACT
Integrin activation and clustering by talin are early steps of cell adhesion. Membrane-bound talin head domain and kindlin bind to the β integrin cytoplasmic tail, cooperating to activate the heterodimeric integrin, and the talin head domain induces integrin clustering in the presence of Mn2+. Here we show that kindlin-1 can replace Mn2+ to mediate β3 integrin clustering induced by the talin head, but not that induced by the F2–F3 fragment of talin. Integrin clustering mediated by kindlin-1 and the talin head was lost upon deletion of the flexible loop within the talin head F1 subdomain. Further mutagenesis identified hydrophobic and acidic motifs in the F1 loop responsible for β3 integrin clustering. Modeling, computational and cysteine crosslinking studies showed direct and catalytic interactions of the acidic F1 loop motif with the juxtamembrane domains of α- and β3-integrins, in order to activate the β3 integrin heterodimer, further detailing the mechanism by which the talin–kindlin complex activates and clusters integrins. Moreover, the F1 loop interaction with the β3 integrin tail required the newly identified compact FERM fold of the talin head, which positions the F1 loop next to the inner membrane clasp of the talin-bound integrin heterodimer.
This article has an associated First Person interview with the first author of the paper.
Stepwise Assembly of Ultrathin Poly(vinyl alcohol) Films on Photoresponsive Diarylethene Crystals
Authors: Chiba Hiroka , Morimoto Masakazu , Irie Masahiro 
Published: October 2020

Abstract
Rodlike two-component mixed crystals composed of 1-(5-methyl-2-phenyl-4-thiazolyl)-2-(5-methyl-2-p-tolyl-4-thiazolyl)perfluorocyclopentene (1a) and 1,2-bis(5-methyl-2-p-tolyl-4-thiazolyl)perfluorocyclopentene (2a) undergo light-driven bending upon alternate irradiation with ultraviolet (UV) and visible light. To provide stability of the crystals in organic solvents, the crystal surface was coated with ultrathin poly(vinyl alcohol) (PVA) films using a stepwise assembly method. The crystals coated with 15 ultrathin PVA layers were stable in n-hexane and no appreciable suppression in the bending performance was observed by the PVA coating. The light-driven bending could be repeated more than 1000 times.
BioSounds: an open-source, online platform for ecoacoustics
Authors: Darras Kevin F.A. , Pérez Noemí , - Mauladi , Hanf-Dressler Tara 
Published: October 2020

Passive acoustic monitoring of soundscapes and biodiversity produces vast amounts of audio recordings. However, the management of these raw data presents technical challenges and their analysis suffers from bottlenecks. A multitude of software solutions exist, but none can perform all the data processing needed by ecologists for analysing large acoustic data sets. The field of ecoacoustics needs a software tool that is free, evolving, and accessible. We take a step in that direction and present BioSounds: an open-source, online platform for ecoacoustics designed by ecologists and built by software engineers. Biosounds can be used for archiving and sharing recordings, manually creating and reviewing annotations of sonant animals in soundscapes, analysing audio in time and frequency, and storing reference recordings for different taxa. We present its features and structure, and compare it with similar software. We describe its operation mode and the workflow for typical use cases such as the analysis of bird and bat communities sampled in soundscape recordings. BioSounds is available from: https://github.com/nperezg/biosounds