bluuebunny/crossref_metadata_2025_split huggingface dataset:
RNA-sequencing reveals positional memory of multipotent mesenchymal stromal cells from oral and maxillofacial tissue transcriptomes
Authors: Onizuka Satoru , Yamazaki Yasuharu , Park Sung-Joon , Sugimoto Takayuki , Sone Yumiko , Sjöqvist Sebastian , Usui Michihiko , Takeda Akira , Nakai Kenta , Nakashima Keisuke , Iwata Takanori 
Published: May 2020

Abstract
Background Multipotent mesenchymal stromal cells (MSCs) can be isolated from numerous tissues and are attractive candidates for therapeutic clinical applications due to their immunomodulatory and pro-regenerative capacity. Although the minimum criteria for defining MSCs have been defined, their characteristics are known to vary depending on their tissue of origin.Results We isolated and characterized human MSCs from three different bones (ilium (I-MSCs), maxilla (Mx-MSCs) and mandibular (Md-MSCs)) and proceeded with next generation RNA-sequencing. Furthermore, to investigate the gene expression profiles among other cell types, we obtained RNA-seq data of human embryonic stem cells (ESCs) and several types of MSCs (periodontal ligament-derived MSCs, bone marrow-derived MSCs, and ESCs-derived MSCs) from the Sequence Reads Archive and analyze the transcriptome profile. We found that MSCs derived from tissues of the maxillofacial region, such as the jaw bone and periodontal ligament, were HOX-negative, while those derived from other tissues were HOX-positive. We also identified that MSX1, LHX8, and BARX1 , an essential regulator of craniofacial development, were strongly expressed in maxillofacial tissue-derived MSCs. Although MSCs may be divided into two distinct groups, the cells originated from over the neck or not, on the basis of differences in gene expression profile, the expression patterns of all CD antigen genes were similar among different type of MSCs, except for ESCs.Conclusions Our findings suggest that MSCs from different anatomical locations, despite meeting general characterization criteria, have remarkable differences in gene expression and positional memory. Although stromal cells from different anatomical sources are generally categorized as MSCs, their differentiation potential and biological functions vary. We suggested that MSCs may retain an original tissue memory about the developmental process, including gene expression profiles. This could have an important impact when choosing an appropriate cell source for regenerative therapy using MSCs.
Processing of human IVF/IVM oocytes for single cell RNA sequencing
Authors: Olsen Kristina W. , Gruhn Jennifer R. , Hoffmann Eva R. , Grøndahl Marie Louise 
Published: May 2020

Abstract
The protocol describes the step-by-step procedure of isolating and preparing human oocytes 1) oocytes donated in connection with IVF/ICSI treatment and 2) in vitro matured (IVM) oocytes from small antral follicles donated in connection to fertility preservation. It details how cumulus cells and the zona pellucida are enzymatically removed to ensure that only the oocyte is analyzed. We have successfully used this protocol in connection with SMART-Seq2 (Takara).This oocyte preparation has recently been used in Sankar et al. 2020 (Sankar et al. 2020).
Effectiveness, Safety, and Major Adverse Limb Events in Atrial Fibrillation Patients with Concomitant Diabetes Mellitus Treated with Non-Vitamin K Antagonist Oral Anticoagulants
Authors: Chan Yi-Hsin , Lee Hsin-Fu , Li Pei-Ru , Liu Jia-Rou , Chao Tze-Fan , Wu Lung-Sheng , Chang Shang-Hung , Yeh Yung-Hsin , Kuo Chi-Tai , See Lai-Chu , Lip Gregory Y. H. 
Published: May 2020

Abstract
Background: Evidence of adverse clinical outcomes for non-vitamin K antagonist oral anticoagulant (NOACs) and warfarin in patients with atrial fibrillation (AF) and diabetes mellitus are limited. We investigated the effectiveness, safety, and major adverse limb events for NOACs versus warfarin among diabetic AF patients.Methods: In this nationwide retrospective cohort study collected from Taiwan National Health Insurance Research Database, we identified a total of 20,967 and 5,812 consecutive AF patients with diabetes taking NOACs and warfarin from June 1, 2012, to December 31, 2017, respectively. We used propensity-score stabilized weighting to balance covariates across study groups.Results: NOAC was associated with a lower risk of major adverse cardiovascular events (MACE) (adjusted hazard ratio (aHR):0.88; [95% confidential interval (CI):0.78-0.99]; P=0.0283), major adverse limb events (MALE) (aHR:0.72;[95%CI:0.57-0.92]; P=0.0083), and major bleeding (aHR:0.67;[95%CI:0.59-0.76];P<.0001) compared to warfarin. NOACs decreased MACE in patients of 75 but not in those aged <75 years (P interaction=0.01), and in patients with ischemic heart disease (IHD) compared to those without IHD (P interaction<0.01). For major adverse limb events, the advantage of risk reduction for NOAC over warfarin persisted in high risk subgroups including age 75 years, chronic kidney disease, IHD, peripheral artery disease, or use of concomitant antiplatelet drugs. Conclusion: Among diabetic AF patients, NOACs were associated with a lower risk of thromboembolism, major bleeding, and major adverse limb events than warfarin. Thromboprophylaxis with NOACs should be considered in the diabetic AF population with a high atherosclerotic burden.
Identifying barriers, facilitators, and implementation strategies for a faith-based physical activity program
Authors: Haughton Jessica , Takemoto Michelle L. , Schneider Jennifer , Hooker Steven P. , Rabin Borsika , Brownson Ross C. , Arredondo Elva M. 
Published: May 2020

Abstract
Background: Community engagement is critical to the acceleration of evidence-based interventions into community settings. Harnessing the knowledge and opinions of community leaders increases the likelihood of successful implementation, scale up, and sustainment of evidence-based interventions. Faith in Action (Fe en Acción) is an evidence-based promotora-led physical activity program designed to increase moderate-to-vigorous physical activity among churchgoing Latina women.Methods: We conducted in-depth interviews using a semi-structured interview guide based on the Consolidated Framework for Implementation Research (CFIR) at various Catholic and Protestant churches with large Latino membership in San Diego County, California to explore barriers and facilitators to implementation of Faith in Action and identify promising implementation strategies for program scale-up and dissemination. We interviewed 22 pastors and church staff and analyzed transcripts using an iterative-deductive team approach. Results: Pastors and church staff described barriers and facilitators to implementation within three domains of CFIR: characteristics of individuals (lack of self-efficacy for and knowledge of physical activity; influence on churchgoers’ behaviors), inner setting (church culture and norms, alignment with mission and values, competing priorities, lack of resources), and outer setting (need for buy-in from senior leadership). From the interviews, we identified four promising implementation strategies for the scale-up of faith-based health promotion programs: 1) health behavior change training for pastors and staff; 2) tailored messaging; 3) developing community collaborations; and 4) gaining denominational support.Conclusions: While churches can serve as valuable partners in health promotion, specific barriers and facilitators to implementation must be recognized and understood. Addressing these barriers through targeted implementation strategies at the adopter and organizational level can facilitate improved program implementation and lead the way for scale-up and dissemination.
Postnatal home visits by health extension workers in rural areas of Ethiopia: a cross-sectional study design
Authors: Tesfau Yemane Berhane , Kahsay Alemayehu Bayray , Gebrehiwot Tesfay Gebregzabher , Medhanyie Araya Abrha , Godefay Hagos 
Published: May 2020

Abstract
Background : In low-income countries like Ethiopia, where families have poor access to or do not utilize the services of formal health care systems, community health workers provide postnatal care services through home visits. However, the extent and effectiveness of home-based postnatal visits by community health workers such as the Ethiopian health extension workers (HEWs) are not well explored. This community -based study aimed to determine the coverage, contents of postnatal home visits and associated factors by health extension workers in Northern Ethiopia. Methods : We conducted a community based cross-sectional study in the rural Districts in Northern Ethiopia from August to September 2018. A total of 705 mothers who gave a live birth in the year preceding the survey were selected using multistage random sampling. A structured questionnaire was applied to collect data by interviewing the mothers. Data were analyzed using SPSS version 22 statistical software. Association of postnatal home visits with possible explanatory variables was investigated using logistic regression. Results : One hundred and two (14.5%) mothers and newborns received PNC home visit within three days after birth from HEW and 170(24.1%) reported postnatal home visits within 42 days. Among the mothers who received postnatal home visits, 6.5% measured their blood pressure, 11.2% measured their temperature, 20% counseled about family planning, 16.5% counseled on newborn danger signs, 11.2% counseled on the skin to skincare of the newborn and 14.1% of their newborns were measured their weight at home. Mothers who received at least one home visit during pregnancy (AOR, 7.49; CI 3.55-15.80), participated in pregnant women forum (AOR, 3.16; CI 1.67-5.99), notified their birth (AOR, 6.16; CI 3.50-10.84) and those members of community health insurance (AOR, 1.87; CI 1.13-3.10) were factors associated with postnatal home visit by a health extension worker. Conclusion : The coverage of postnatal home visits by health extension workers remains low in rural districts of Northern Ethiopia. The existing health systems should consider interventions that improve pregnancy and birth notification strategies and more efforts should be made at improving community-based participation and linkages with community health workers.
High Level of Vaccination and Protection Against Hepatitis B with Low Rate of Hepatitis C HCV infection Markers among Hospital Health Care Personnel in North of Iran: A cross-Sectional study.
Authors: Saffar Hiva , Khoshayand Negar , Parsaei Mohammad-Reza , Ghorbani Gholam-Reza , Aarabi Mohsen , Ghara Ali-Asghar Nadi , Saffar mohammed-Jafar 
Published: May 2020

Abstract
Background: hepatitis B virus (HBV) and C virus (HCV) are among the leading causes of mortality worldwide. Health care personnel (HCP) are subjected to increased risk of these infections. Therefore, HBV vaccination and post-vaccination serologic testing (PVST) are recommended for them. Our Objectives in this study were investigate how well the vaccination guidelines for hospital HCPs were implemented. Moreover, the prevalence rates of HBV and HCV infections were calculated. To determine the presence of immunological memory, vaccinated personnel negative to antibody against HB surface antigen with one dose of HB vaccine were boosted. Methods: From 1 July to 30 November 2017, a cross-Sectional study among HCPs working in public hospitals were conducted. All HCPs from various professional categories potentially at risk of exposure to contaminated sources were included. The information was gathered via interview and self-administered questionnaire. The questions were focused on the demographic characteristics, HB vaccination and immunity status and time elapsed since initial vaccination series, and frequency of needelstick injuries during the past 12 months of their work. Moreover, the prevalence rate of HBV and HCV infections were calculated. To determine the presence of immunological memory, subjects negative to HBV seromarkers received a booster dose of the vaccine.Results: A total of 186 out of 766 participants were male and nurses comprised 71% of personnel. Although all HCP were vaccinated, 84% of them completed the course and less than 5% of them received PVST. According to the results, 0.78%, 4.6%, and 83% were serologically positive to HBV surface antigen, antibodies against HBV core, and S antigens, respectively. Approximately, 91% of seronegative participants responded to a booster dose and only 0.91% of the personnel was Anti-HCV positive.Conclusion: Most HCP received full HBV vaccination course. Although a minority did PVST, the HBV vaccine-induced long-term protection and HB vaccine booster were not required. Therefore, policies should be made to increase the rate PVST after immunization. According to the results, the HCV infection rate was low and thus pre-recruitment screening was not necessary.
Resistance phenotype and molecular epidemiology of Carbapenem-resistant Klebsiella pneumoniae isolates in Shanghai
Authors: Zhang Wenxia , Chen Hongyou , Chen Chen , Chen Junhao , Li Lingxia , Chen Min , Zhang Jue 
Published: May 2020

Abstract
Background: The emergence and wide global spread of carbapenem-resistant Klebsiella pneumoniae (CRKP) isolates are of great concern, and the aim of this study was to investigate drug resistance, molecular epidemiology, and genetic relationship of CRKP isolates from patients in Shanghai, China. Methods: A retrospective study was conducted from April 2018 to July 2019, and a total of 133 CRKP isolates were collected. Antimicrobial susceptibility was determined by VITEK-2 automated microbiology analyzer platform (bioMérieux, France) and the broth microdilution method. Polymerase chain reaction (PCR) assays were used to investigate the presence of drug resistance genes. A modified carbapenem inactivation method (mCIM) was performed to detect carbapenemases. Multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE) were conducted for genetic relatedness of 50 CRKP isolates selected. Results: Among 670 isolates of K. pneumoniae, 133 (19.85%) strains were identified as carbapenem-resistant K. pneumoniae (CRKP), of which, 76.69% (102/133) strains were isolated from ICUs. All the 133 CRKP isolates were found to be carbapenemase-producers and harbor blaKPC-2 gene. No other carbapenemase genes of blaNDM, blaOXA−48, blaVIM, and blaIMP were detected. Furthermore, β-lactamase genes of blaSHV, blaCTX, and blaTEM were the most common resistance-associated genes among these KPC-2 producing isolates. All the 133 CRKP strains displayed more than 95% of resistance to cephalosporins and carbapenems, except for gentamicin, Trimethoprim-sulfamethoxazole, amikacin, tigecycline and colistin. The most common sequence type was ST11, accounting for 90.0% of the 50 CRKP selected, followed by ST15 (10%). PFGE analysis clustered the 50 KPC-2-producing isolates into seven (A-G) distinct clonal clusters at 85% cut off. Of which, cluster A and G were the two major clusters, accounting for the majority of the strains collected in emergency ICU and neurosurgical ICU. And all the strains of cluster D and E were collected in cardiothoracic surgery ICU, expect for one strain collected in one outpatient. Conclusion: The KPC-2-producing K.pneumoniae belonged to ST11 was widely disseminated in ICUs, and active and effective surveillance of infection control strategies was initiated to limit the spread of CRKP strains.
Neuroprotection by hypercapnia in brain ischemic injury followed by persistent hypoxia: an in vivo and in vitro experimental study
Authors: Yang Wanchao , Wang Qi , Wu Xiaohong , Li Tingting , Wan Qiang , Li Wen-Zhi 
Published: May 2020

Abstract
Backgrounds: Therapeutic hypercapnia was shown to have a potential neuroprotective role in our previous studies in a rat model of ischemia followed by hypoxia; however, it is unknown how hypercapnia affects blood-brain barrier (BBB) function under hypoxic conditions in cerebral ischemia. We aimed to observe the BBB permeability changes in response to cerebral ischemia followed by acute hypoxia or hypercapnic hypoxia using in vivo and in vitro models.Methods: Adult rats underwent unilateral common carotid artery ligation, at 60 min of ligation, they were exposed to systemic hypoxia with ventilation of 15% oxygen (O2) combined with 8% carbon dioxide (CO2) for 180 min. Cerebral blood flow, BBB integrity, infarct volume and behavior were assessed in this study. In vitro, rat brain microvascular endothelial cells (BMECs) were isolated and cultured under O2 (1% or 21%) with or without 15% CO2 for 6 h. Cell viability and transendothelial electrical resistance (TEER) were measured. The ZO-1 and occludin protein levels were explored in BMECs by Western blotting.Results: Arterial blood O2 (PaO2) tensions averaged 56.1 mmHg during simple hypoxia, and arterial blood CO2 tensions (PaCO2) were maintained at normal values or 60–80 mmHg. Hypercapnia treatment significantly reduced brain infarct volume and pathophysiological changes in hypoxic ischemia rats. Furthermore, in the in vitro experiment, hypercapnia significantly improved the growth condition of BMECs, reduced endothelial cell permeability and attenuated the loss of ZO-1 and occludin protein in BMECs induced by hypoxia. Conclusions: Hypercapnia exerts beneficial effects on the BBB permeability in the rat model of hypoxic-ischemic injury and recovers the neurologic status especially within one week, possibly by preventing the loss of tight junction proteins.
Simulation design of acceleration sensor with multi-stage induction system
Authors: Feng Jiacheng 
Published: May 2020

Abstract
Acceleration sensor can monitor acceleration and trigger switches i n a dynamic environment. Based on the coupling mechanism of magneto-mechanical, this paper presents the design of an acceleration sensor . With the characteristics of integrated mechanism of perception and triggering, no power consumption and high robustness, it can be widely used in active and passive vehicle safety, robot and dynamic monitoring system. In order to improve the robustness of system, the closing and holding mechanism of switch is designed. Considering the comprehensive effects of elastic element, suction contact and permanent magnet, the paper built the mathematical model of sensor and the threshold searching algorithm. The simulation results show that the acceleration switch has the characteristic of sensitivity in specific dynamic signal, and it works well in overload and vibration disturbance environment. In conclusion, the paper presents a new design model and method for bilateral multi- stage acceleration sensor.
Canine helminthiases and associated risk factors in Kigali city, Rwanda
Authors: Ntampaka Pie , Niragire François , Nyaga Philip Njeru , Habarugira Gervais 
Published: May 2020

Abstract
Background

Despite that canine helminthiases pose a public health risk to humans and livestock, the prevalence of canine helminthiases in Rwanda is unknown. This study aimed to determine the prevalence of canine helminthiases and to identify the risk factors of such infections in Kigali, the capital city of Rwanda. A cross-sectional study involved 93 dogs selected across Kigali city. Faecal samples were collected from clinically healthy dogs and eggs worms were identified using McMaster technique. Risk factors for canine helminthiases were analysed by multivariable binary logistic regression analysis.
Results

The overall prevalence of intestinal helminthiases in dogs was 39.8%. The most prevalent species was Ancylostoma spp (32.3%, n = 93). About 41.9% (n = 31) of dogs suffering from ancylostomosis and/or toxocarosis had high egg counts per gram (EPG) of faeces (≥ 550). Approximately 97.3% (n = 37) of dogs infected with helminths had mono-infection. Logistic regression analysis showed that dog's age and location were significantly associated with the prevalence of canine helminthiases. The adjusted odds ratio (AOR) of dogs suffering from helminthiases was more than 61% lower in dogs dewormed once to twice a year compared to those who were never dewormed. The AOR was more than 42% lower for restricted dogs compared to non-restricted ones.
Conclusions

All the identified helminths are zoonotic and they pose a public health risk to humans. There is urgent need of awareness among dog owners on the role of dogs in transmitting zoonotic helminthiases to other animals and humans as well as effective control measures.
Inverse correlation between Interleukin-34 and gastric cancer, a potential biomarker for prognosis
Authors: Liu Qinghua , Zhang Ying , Zhang Jiwei , Tao Kun , Hambly Brett D , Bao Shisan 
Published: May 2020

Abstract
Background Gastric cancer (GC) is a malignancy with high morbidity/mortality, partly due to a lack of reliable biomarkers for early diagnosis. It is important to develop reliable biomarker(s) with specificity, sensitivity and convenience for early diagnosis. The role of tumour-associated macrophages (TAMs) and survival of GC patients are controversial. Macrophage colony stimulating factor (MCSF) regulates monocytes/macrophages. Elevated MCSF is correlated with invasion, metastasis and poor survival of tumour patients. IL-34, a ligand of the MCSF receptor, acts as a “twin” to MCSF, demonstrating overlapping and complimentary actions. IL-34 involvement in tumours is controversial, possibly due to the levels of MCSF receptors. While the IL34/MCSF/MCSFR axis is very important for regulating macrophage differentiation, the specific interplay between these cytokines, macrophages and tumour development is unclear.Methods A multi-factorial evaluation could provide more objective utility, particularly for either prediction and/or prognosis of gastric cancer. Precision medicine requires molecular diagnosis to determine the specifically mutant function of tumours, and is becoming popular in the treatment of malignancy. Therefore, elucidating specific molecular signalling pathways in specific cancers facilitates the success of a precision medicine approach. Gastric cancer tissue arrays were generated from stomach samples with TNM stage, invasion depth and the demography of these patients (n = 185). Using immunohistochemistry/histopathology, MCSF, IL-34 and macrophages were determined.Results We found that IL-34 may serve as a predictive biomarker, but not as an independent, prognostic factor in GC; MCSF inversely correlated with survival of GC in TNM III‑IV subtypes. Increased CD68+TAMs were a good prognostic factor in some cases and could be used as an independent prognostic factor in male T3 stage GC.Conclusion Our data support the potency of IL-34, MCSF, TAMs and the combination of IL34/TAMs as novel biological markers for GC, and may provide new insight for both diagnosis and cellular therapy of GC.
Deep Double-Pilot-Based Hybrid Precoding in UAV-Enabled MmWave Massive MIMO
Authors: Hong Ziyao , Li Ting , Li Fei 
Published: May 2020

Abstract
Unmanned aerial vehicle (UAV) enabled communication system provides flexibility and reliability compared to conventional ones. Millimeter wave and massive MIMO have widely been researched since recent years, which are promising techniques for the next and even the later generation communication system. Hybrid precoding, as a method to reduce the high cost in hardware and power brought by massive antenna array, develops fiercely and is often combined to deep learning, a kind of popular optimization tool, which brings overwhelming performance. On the other hand, there are not so many attentions about the hybrid precoding in time-varying millimeter wave massive MIMO, which is necessary to be considered in a UAV-enabled communication scenario because the performance will degrade seriously if the channel changed while the transmitter and receiver use the precoding matrix corresponding to the expired channel, yet. In this paper, we propose a double-pilot-based hybrid precoding system, which completes analog precoding and digital precoding separately--predicting the previous one using deep learning structure and updating equivalent channel frequently for the post one by enhancing the frequency of equivalent channel estimation.