bluuebunny/crossref_metadata_2025_split huggingface dataset:
Molecular and Clinical Characterization of LAG3 in Breast Cancer Through 2994 Samples
Authors: Liu Qiang , Qi Yihang , Zhai Jie , Kong Xiangyi , Wang Xiangyu , Fang Yi , Wang Jing 
Published: June 2020

Abstract
Background Despite the promising impact of cancer immunotherapy targeting CTLA4 and PD1/PDL1, a large number of cancer patients fail to respond. LAG3 (Lymphocyte Activating 3), also named CD233, is a protein Coding gene served as alternative inhibitory receptors to be targeted in the clinic. The impact of LAG3 on immune cell populations and co-regulation of immune response in breast cancer remained largely unknown. Methods To characterize the role of LAG3 in breast cancer, we investigated transcriptome data and associated clinical information derived from a total of 2994 breast cancer patients. Results We observed that LAG3 was closely correlated with major molecular and clinical characteristics, and was more likely to be enriched in higher malignant subtype, suggesting LAG3 was a potential biomarker of triple-negative breast cancer. Furthermore, we estimated the landscape of relationship between LAG3 and ten types of cell populations in breast cancer. Gene ontology analysis revealed LAG3 were strongly correlated with immune response and inflammatory activities. We investigated the correlation pattern between LAG3 and immune modulators in pan-cancer, especially the synergistic role of LAG3 with other immune checkpoints members in breast cancer. Conclusions LAG3 expression was closely related to malignancy of breast cancer and might serve as a potential biomarker; LAG3 might plays an important role in regulating tumor immune microenvironment, not only T cells, but also other immune cells. More importantly, LAG3 might synergize with CTLA4, PD1/ PDL1 and other immune checkpoints, thereby lending more evidences to combination cancer immunotherapy by targeting LAG3, PD1/PDL1, and CTLA4 together.
Level of CRP predicts need for respiratory support in hospitalized COVID-19 patients
Authors: Markowicz Samuel , Donat Sunniva , Glaude Eddy-Laurent , Pommier Jean-David , Curlier Elodie , Guyomard-Rabenirina Stéphanie , Kikweta Jeanos , Amanako Junior , Ouassou Amael , Roger Pierre-Marie 
Published: June 2020

Abstract
PurposePredictors of disease severity in COVID-19 are essential to identify patients requiring hospitalization. Our aim was to determine the relationship of C-reactive protein (CRP) with the need for respiratory support.MethodsThis was a retrospective monocentric study of all patients hospitalized for confirmed COVID-19 pneumonia. CRP was measured on admission in the serum. Patients were classified using the Pneumonia Severity Index (PSI) and a 3-stage internal severity score (ISS) based upon respiratory parameters. Chest CT scans performed on admission were analysed following guidelines. Correlations of CRP levels with disease severity, radiological score, oxygen or mechanical ventilation requirement, and death were studied.Results61 patients were included from March 13th to April 8th, 2020. CRP levels were better related to the ISS compared to the PSI, being 18 (5-54), 130 (50-147) and 169 (97-241) mg/L respectively for the low, intermediate and high severity groups (p = 0.004 and p = 0.017). Analysis of the 51 available CT scans found a smaller correlation between CRP levels and radiological score (p < 0.05). The CRP levels were related to oxygen requirement (n = 50, p = 0.001), mechanical ventilation (n = 20, p = 0.004) and death (n = 10, p= 0.001).ConclusionCRP level on admission was a good marker of clinical and radiological severity in COVID-19 pneumonia, and could be used to identify patients needing hospitalization and intensive care.
Real-world evaluation of a computed tomography-first triage strategy for suspected Coronavirus disease 2019 in outpatients with fever in Japan: an observational cohort study
Authors: Miyake Shigeta , Higurashi Takuma , Jono Takashi , Akimoto Taisuke , Ogawa Fumihiro , Oi Yasufumi , Tanaka Katsushi , Hara Yu , Kobayashi Nobuaki , Kato Hideaki , Yamashiro Tsuneo , Utsunomiya Daisuke , Nakajima Atsushi , Yamamoto Tetsuya , Maeda Shin , Kaneko Takeshi 
Published: June 2020

Abstract
Background: The Coronavirus disease 2019 pandemic continues to spread worldwide. Because of the absence of reliable rapid diagnostic systems, patients with symptoms of Coronavirus disease 2019 are treated as suspected of the disease. Use of computed tomography findings in Coronavirus disease 2019 are expected to be a reasonable method for triaging patients, and computed tomography-first triage strategies have been proposed. However, clinical evaluation of a computed tomography-first triage protocol is lacking.The aim of this study is to investigate the real-world efficacy and limitations of a computed tomography-first triage strategy in patients with suspected Coronavirus disease 2019.Methods: This was a single-center cohort study evaluating outpatients with fever who received medical examination at Yokohama City University Hospital, prospectively registered between 9 February and 5 May 2020. We treated according to the computed tomography-first triage protocol. The primary outcome was efficacy of the computed tomography-first triage protocol for patients with fever in an outpatient clinic. Efficacy of the computed tomography-first triage protocol for outpatients with fever was evaluated using sensitivity, specificity, positive predictive value, and negative predictive value. We conducted additional analyses of the isolation time of feverish outpatients and final diagnoses.Results: In total, 108 consecutive outpatients with fever were examined at our hospital. Using the computed tomography-first triage protocol, 48 (44.9%) patients were classified as suspected Coronavirus disease 2019. Nine patients (18.8%) in this group were positive for severe acute respiratory syndrome coronavirus 2 using polymerase chain reaction; no patients in the group considered less likely to have Coronavirus disease 2019 tested positive for the virus. The sensitivity, specificity, positive predictive value, and negative predictive value of our computed tomography-first triage protocol for Coronavirus disease 2019 were 100%, 60.2%, 18.8%, and 100%, respectively. The protocol significantly shortened the duration of isolation for the not-suspected versus the suspected group (70.5 vs. 1037.0 minutes, P < .001). Conclusions: Our computed tomography-first triage protocol was acceptable for screening patients with suspected Coronavirus disease 2019. This protocol will be helpful for appropriate triage, especially in areas where polymerase chain reaction is inadequate.
Bioinformatics analysis reveals the ceRNA co-expression network in tumor microenvironment and prognostic biomarkers in Sarcomas
Authors: Zou Dandan , Wang Yang , Wang Meng , Zhao Bo , Hu Fei , Li Yanguo , Zhang Bingming 
Published: June 2020

Abstract
Background

Sarcomas (SARCs) are rare, heterogeneous mesenchymal neoplasia. To understand the tumor microenviroment (TME) and identify potential biomarkers for prognosis that associated with TME of SARCs might provide effective clues for immune therapy.
Methods

We evaluated the immune scores and stromal scores by using the RNA sequencing dataset of SARCs patients from The Cancer Genome Atlas (TCGA) database and the ESTIMATE algorithm. Then the differentially expressed mRNAs (DEGs), miRNAs (DEMs) and lncRNAs (DELs) were filtered after comparing the two high- and low- scores groups. Next, based on these DERNAs, we established a competing endogenous RNA (ceRNA) network and explored the prognostic roles of biomarkers involved in the network with the help of bioinformatics analysis.
Results

High immune scores were significantly associated with favorable overall survival of SARCs patients. Next, a total of 328 DEGs, 18 DEMs and 67 DELs that commonly regulated in the immune and stromal scores groups were obtained. And for the DEGs, some of the Gene Ontology (GO) terms and pathways were mainly associated with immune processes. A ceRNA network were constructed with 142 nodes and 424 edges, in which hsa-miR-9-5p, hsa-miR-490-3p, hsa-miR-133a-3p, hsa-miR-133b and hsa-miR-129-5p were the top 5 nodes. Additionally, the protein-protein Interaction (PPI) network identified MMP9, TYROBP, CSF1, CXCR4, FBN1, FLNA, PDGFRB, CYBB, FCGR3A and MYH11 as hub nodes with considerable importance that functioned in the network. Finally, the Kaplan-Meier survival analysis demonstrated that 9 mRNAs (APOL1, EFEMP1, LYZ, MEDAG, MYH11, RARRES1, TNFAIP2, TNFSF10 and ZNF385A), 2 miRNAs (hsa-miR-9-5p and hsa-miR-183-5p) and 3 lncRNAs (CTD-2228K2.7, HOTAIRM1 and NCF1C) were closely associated with the overall survival of SARCs patients.
Conclusions

Taken together, our study confirmed that the prognosis value of immune scores for SARCs patients, also we identified a list of TME-related biomarkers which might contribute to prognostic prediction and help improve the efficacy of immune therapy.
Improved inflammatory bowel disease, wound healing and normal of oxidative burst under treatment with empagliflozin in glycogen storage disease type Ib
Authors: Grünert Sarah Catharina , Elling Roland , Maag Bärbel , Wortmann Saskia B. , Derks Terry G. J. , Hannibal Luciana , Schumann Anke , Rosenbaum-Fabian Stefanie , Spiekerkoetter Ute 
Published: June 2020

Abstract
Background

Glycogen storage disease type Ib (GSD Ib) is a rare inborn error of glycogen metabolism due to mutations in SLC37A4. Besides a severe form of fasting intolerance, the disorder is usually associated with neutropenia and neutrophil dysfunction causing serious infections, inflammatory bowel disease, oral, urogenital and perianal lesions as well as impaired wound healing. Recently, SGLT2 inhibitors such as empagliflozin that reduce the plasma levels of 1,5-anhydroglucitol have been described as a new treatment option for the neutropenia and neutrophil dysfunction in patients with GSD Ib.
Results

We report on a 35-year-old female patient with GSD Ib who had been treated with G-CSF for neutropenia since the age of 9. She had a large chronic abdominal wound as a consequence of recurrent operations due to complications of her inflammatory bowel disease. Treatment with 20 mg empagliflozin per day resulted in normalisation of the neutrophil count and neutrophil function even after termination of G-CSF. The chronic abdominal wound that had been unchanged for 2 years before the start of empagliflozin nearly closed within 12 weeks. No side effects of empagliflozin were observed.
Conclusion

SGLT2 inhibitors are a new and probably safe treatment option for GSD Ib-associated neutropenia and neutrophil dysfunction. We hypothesize that restoration of neutrophil function and normalisation of neutrophil apoptosis leads to improvement of wound healing and ameliorates symptoms of inflammatory bowel disease.
Serum procalcitonin levels predict disseminated intravascular coagulation in patients with sepsis shock: A retrospective cohort study
Authors: Fu Guang , He Xi-si , Li Hao-li , Zhan Hai-chao , Lu Jun-fu , Chen Yan-hong , Yang Zi-Long , Huang Jia-jia , Feng Yong-wen , Luan Ying-yi , Wu Ming 
Published: June 2020

Abstract
Background

Complication of disseminated intravascular coagulation (DIC) is a determinant of the prognosis in patients with sepsis shock. Procalcitonin (PCT) has been advocated as a marker of bacterial sepsis. The purpose of this study was to evaluate the relationship between serum PCT levels and DIC with sepsis shock
Methods

A cohort study was designed which included patients that admitted in intensive care unit (ICU) between January 1, 2015 and December 31, 2018 and the follow-up to discharge. 164 septic shock patients were divided into DIC and non-DIC groups according to international society of thrombosis and homeostasis (ISTH). PCT was measured at the admission to ICU, and all the participants received routine biochemical coagulation test subsequently.
Results

PCT levels were considerably higher in septic shock patients who developed DIC than those who did not (54.6[13.6–200]vs12.6[2.4–53.3]ng/ml), respectively, P < 0.001). Multivariable logistic regression model revealed that PCT level was significantly associated with risk of DIC independent of conventional risk factors. In addition, curve fitting showed a linear relationship between PCT and DIC score. The Receiver Operating characteristic(ROC) curve suggested that the optimal cut-off point for PCT to predicting DIC induced by septic shock was 42.0 ng/ml, and the area under the curve (AUC) was 0.701(95% CI [0.619–0.784], P < 0.001). More importantly, incorporating PCT with other risk factors into the prediction model significantly increased the AUC for prediction of DIC induced by sepsis shock (0.801vs 0.706; P = 0.012).
Conclusions

Our study suggests that PCT levels on admission is significantly and independently associated with DIC development subsequently with septic shock, combining PCT levels with other risk factors could significantly improve the prediction of DIC induced by sepsis shock.
Embarked on social processes (the rivers) in dynamic and multilevel networks (the boats)
Authors: Lazega Emmanuel 
Published: June 2020

Abstract
This paper is the written text underlying the keynote presentation at the Sunbelt XXXVIII in Utrecht, 2018. It presents a neo-structural approach to social processes in the organizational society and the usefulness of the analyses of multilevel networks to understand how we navigate these processes and are made aware of them when we face cooperation dilemmas. Empirical illustrations look at how multilevel networks and relational infrastructures are useful to research a process such as coopetitive learning in science, business and government. A conclusion focuses on the role of multilevel relational infrastructures in institutional entrepreneurship, social change and politics, as well as on our responsibility to develop our knowledge of these social processes and multilevel relational infrastructures as open science.
Influence of Climate on the Spatiotemporal Distribution of Malaria in Thulamela Municipality, Limpopo Province, South Africa
Authors: Makondo Lungile , Adeola Abiodun , Makgoale Thabo , Botai Joel , Adisa Omolola , Botai Christina 
Published: June 2020

Background:
Malaria, though curable, continues to be a major health and socioeconomic challenge. Malaria cases have been on the rise for the last two years in the malaria-endemic region of South Africa. Thulamela Municipality in Limpopo, South Africa, which falls within several municipalities at Vhembe district that are affected by malaria. About 33,448 malaria cases were reported over a period of 20 years (1998 January-2018 December).

Objective:
The study aims to determine the influence of climate on the spatiotemporal distribution of malaria cases in Thulamela Municipality for the last two decades (1998 January-2018 December).

Methods:
The analysis is divided into two sections, including temporal and spatial distribution of malaria cases, and the correlating climatic and environmental factors. Time series analysis is conducted to determine the variations of malaria and climate. Malaria and climatic factors (rainfall, maximum temperature, minimum temperature) were globally correlated using matrix scatterplot spearman correlation with a certain significance level. The Ordinary Least Squares (OLS) regression was performed to determine the significant climate factors that locally affect the spatial distribution of malaria cases. The local environmental factor (rivers) was analyzed using buffering and terrain analysis.

Results:
A positive spearman correlation of the time series was found with the significance level of 0.01. The climate variables were not strongly significant to the spatial distribution of malaria at the village level. The villages which continued to record high malaria cases were in proximity to rivers by 2km. The Thulamela municipality falls within 20-30°C, which is essential for the incubation of mosquitoes and transmission of malaria. The areas receiving about 125 to 135 mm of total monthly rainfall record high malaria cases. The temperature, rainfall, and rivers are important factors for malaria transmission.

Conclusion:
Knowledge of the drivers of the spatiotemporal distribution of malaria is essential for a predicting system to enhance effective malaria control in communities such as the Thulamela municipality.
REVISÃO DE LITERATURA E REVISÃO SISTEMÁTICA: UMA ANÁLISE OBJETIVA
Authors: Gomes Nazareth Camila Cristine 
Published: June 2020

RESUMO A pesquisa contou com um total de 57 (cinquenta e sete) trabalhos científicos que incluíram artigos científicos e editoriais de revistas científicas. Ao todo foram selecionados 28 (vinte e oito) trabalhos científicos com base no delineamento da pesquisa de revisão sistemática, sendo: 22 (vinte e dois) trabalhos científicos cujo eixo era a temática da revisão de literatura e 7 (sete) trabalhos científicos discutiam especificamente as diferenças e semelhanças entre revisão de literatura e revisão sistemática. Estes sete artigos serviram de base para o estudo no presente trabalho já que versam exclusivamente sobre o assunto. O objetivo do presente trabalho é realizar uma análise objetiva das diferenças entre a revisão de literatura comparada com a revisão sistemática de literatura. Palavras Chave: Revisão de Literatura; Revisão Sistemática; Revisão Narrativa ABSTRACT A survey included a total of 57 (fifty-seven) scientific papers, including scientific articles and editorials from scientific journals. When 28 (twenty-eight) scientific papers were selected based on systematic review research, 22 (twenty-two) scientific papers focused on literature review and 7 (seven) scientific studies discussed as the similarities between literature review and systematic review. These seven articles served as the basis for the study in the present work since they deal exclusively with the subject.The objective of the present work is to carry out an objective analysis of the differences between a literature review compared to a systematic review. Key words: Literature Review; Systematic Review; Narrative Review
A FOTOPOLIMERIZAÇÃO DE COMPÓSITOS ODONTOLÓGICOS ALÉM DA POTÊNCIA LUMINOSA
Authors: Ribeiro de Magalhães Filho Thales 
Published: June 2020

O objetivo deste estudo foi mostrar que a potência da luz das unidades de fotopolimerização (LED) não é a única ou a propriedade mais importante para uma polimerização satisfatória de compósitos dentários. A distribuição regular e o ângulo de incidência dos raios de luz também são igualmente responsáveis por uma polimerização mais completa e profunda. Esta revisão de literatura mostra que avançamos o suficiente na potência da luz emitida pelos LEDs, mas no que diz respeito à regularidade e ângulo dos raios emitidos, ainda temos muito a avançar. Novos compósitos com cargas reflexivas e preocupações com a diferença de índice de refração entre as cargas e a matriz são importantes para melhorar essa polimerização, mas os LEDs com vários comprimentos de onda podem complicar ainda mais a distribuição regular dos raios de luz emitidos. Dispositivos com diferentes LEDs emitindo raios de luz com diferentes comprimentos de onda não distribuem essas ondas no modo regular. Portanto, parte da restauração pode estar sub polimerizada, tornando-se menos resistente aos esforços mastigatórios e à infiltração. O estudo também relembra propostas de avaliação científica dessa distribuição regular e conclui que os LEDs modernos emitem raios de luz com grande potência e irradiância, mas ainda são irregulares na distribuição dos raios de luz emitidos.Palavras-chave: fotopolimerização, conprimento de onda, irradiância, resina composta.PHOTOPOLIMERIZATION OF DENTAL COMPOSITES BEYOND THE LIGHT POWER EMITED BY LED LIGHT CURING UNITSABSTRACT: The objective of this study was to show that light power of light curing units (LCU) is not the only or the most important property for a satisfactory polymerization of dental composites. Regular distribution and angle of incidence of the light rays are also equally responsible for a fuller and deeper polymerization. This literature review shows that we advanced enough in the power of light emitted by LCUs, but as regards the regularity and angle of the emitted rays, we still have much to advance. New composites with reflective fillers and concerns about the difference of refractive index between the fillers and the matrixare important help to improve this polymerization, but LCUs with several wavelengths can further complicate the regular distribution of emitted light rays. Devices with different LEDs emitting light rays with different wavelengths does not distribute these waves in a regular mode. Therefore, part of the restoration can be under polymerized, becoming less resistant to masticatory efforts and infiltration as well. The study also recalls proposals for scientific evaluation of this regular distribution and concludes that modern LCUs emit light rays with great power and irradiance, but are still irregular in the distribution of emitted light rays.Keywords: photopolymerization, wavelength, Irradiance, composite.
Defining Abdominal Obesity as a Risk Factor for Coronary Heart Disease in the U.S.: Results From the Hispanic Community Health Study/Study of Latinos (HCHS/SOL)
Authors: Chirinos Diana A. , Llabre Maria M. , Goldberg Ronald , Gellman Marc , Mendez Armando , Cai Jianwen , Sotres-Alvarez Daniela , Daviglus Marta , Gallo Linda C. , Schneiderman Neil 
Published: June 2020

OBJECTIVE
Various organizations have highlighted the need to examine whether abdominal obesity cut points are appropriate for identification of cardiovascular risk among ethnic minority adults, particularly Hispanic/Latino adults living in Western societies. This study aimed 1) to establish optimal definitions for abdominal obesity among Hispanics/Latinos and 2) to determine the level of agreement between the presence of metabolic syndrome diagnosed by the current Joint Interim Statement (JIS) definition and an updated definition with optimal abdominal obesity cut points.

RESEARCH DESIGN AND METHODS
The sample included 16,289 adults who self-identified as Hispanic/Latino ages 18–74 years enrolled in the Hispanic Community Health Study/Study of Latinos (HCHS/SOL). Receiver operating characteristic curves were used to derive sensitivity and specificity values. The largest sum of sensitivity plus specificity was used to determine appropriate cut points.

RESULTS
Among U.S. Hispanic/Latino adults, waist circumference cut points of &gt;102 cm in men (in line with current JIS criteria) and &gt;97 cm (9 points higher than JIS criteria) in women provide optimal discrimination for cardiovascular risk as judged by the presence of coronary heart disease. When using these cut points to create an updated metabolic syndrome definition among women, we found disagreement between our updated definition and the current JIS criteria. The prevalence of the metabolic syndrome was overestimated by ∼5 percentage points among women based on JIS criteria in comparison with our definition.

CONCLUSIONS
Our results suggest that the current recommendations for waist circumference cut points may not be appropriate for U.S. Hispanic/Latino women.