bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Mitochondrial Permeability Transition Pore Activates the Mitochondrial Unfolded Protein Response and Promotes Aging
Authors: Angeli Suzanne , Foulger Anna C. , Chamoli Manish , Peiris Tanuja Harshani , Gerencser Akos A. , Shahmirzadi Azar Asadi , Andersen Julie K. , Lithgow Gordon J. 
Published: November 2020

SUMMARYMitochondrial activity determines aging rate and the onset of chronic diseases. The mitochondrial permeability transition pore (mPTP) is a pathological pore in the inner mitochondrial membrane thought to be composed of the F-ATP synthase (complex V). OSCP, a subunit of F-ATP synthase, helps protect against mPTP formation. How the destabilization of OSCP may contribute to aging, however, is unclear. We have found that loss OSCP in the nematodeCaenorhabditis elegansinitiates the mPTP and shortens lifespan specifically during adulthood, in part via initiation of the mitochondrial unfolded protein response (UPRmt). Pharmacological or genetic inhibition of the mPTP inhibits the UPRmtand restores normal lifespan. Loss of the putative pore-forming component of F-ATP synthase extends adult lifespan, suggesting that the mPTP normally promotes aging. Our findings reveal how an mPTP/UPRmtnexus may contribute to aging and age-related diseases and how inhibition of the UPRmtmay be protective under certain conditions.
Rapid conversion of replicating and integratingSaccharomyces cerevisiaeplasmid vectors via Cre recombinase
Authors: Nickerson Daniel P. , Quinn Monique A. , Milnes Joshua M. 
Published: November 2020

ABSTRACTPlasmid shuttle vectors capable of replication in bothSaccharomyces cerevisiaeandEscherichia coliand optimized for controlled modificationin vitroandin vivoare a key resource supporting yeast as a premier system for genetics research and synthetic biology. We have engineered a series of yeast shuttle vectors optimized for efficient insertion, removal and substitution of plasmid yeast replication loci, allowing generation of a complete set of integrating, low copy and high copy plasmids via predictable operations as an alternative to traditional subcloning. We demonstrate the utility of this system through modification of replication loci via Cre recombinase, bothin vitroandin vivo, and restriction endonuclease treatments.
Isolation and biophysical characterization of GSU0105, a triheme c-type cytochrome fromGeobacter sulfurreducens
Authors: Brittain Tyler J. , O’Malley Matthew C. , Swaim Coleman M. , Fink Reilly A. , Kokhan Oleksandr 
Published: November 2020

AbstractC-type cytochromes play an important role in respiration of dissimilatory metal-reducing bacteria. They form extended conduits for charge transfer between the cellular metabolism and external electron acceptors such as particles of iron oxide, metal ions, and humic substances. Out of more than a hundred c-type cytochromes inGeobacter sulfurreducens, only a small fraction has been previously characterized. Here we present our results on expression and biophysical characterization of GSU0105, a novel 3-heme cytochrome, important for Fe(III) respiration inG. sulfurreducens. We successfully cloned the gene and achieved ~3 mg/L of culture GSU0105 expression inE.coli. Despite a similar size (71 amino acids) and the same number of c-type hemes to the members of the cytochrome (cyt) c7family, multiple sequence alignment suggests that GSU0105 does not belong to the cyt c7family. UV-Vis spectroscopy revealed typical c-type cytochrome spectral features, including a weak iron-sulfur charge transfer band suggesting that at least one heme is ligated with a methionine residue. Far UV circular dichroism studies demonstrate approximately 35% content of α-helices and β-sheets, each, as well as thermal aggregation occurring above 60 °C. A combination of SAXS and analytical size exclusion chromatography data shows that GSU0105 is monomeric in solution. Finally, affinity pull-down assays demonstrate high binding affinity to PpcD and weaker binding to the other members of the cyt c7family.
BRAF controls the effects of metformin on neuroblast cell divisions inC. elegans
Authors: Qu Zhi , Ji Shaoping , Zheng Shanqing 
Published: November 2020

AbstractMetformin has demonstrated substantial potential for use in cancer treatments. LKB-AMPK and mTOR are reported to be the main targets of metformin in relation to its ability to prevent cancer cell proliferation. However, the role of metformin in the control of neoplastic cancer cell growth is possibly independent of LKB-AMPK and mTOR. UsingC. elegansas a model, we found that the neuronal Q-cell divisions in L1-arrested worms were suppressed following metformin treatment in AMPK-deficient mutants, suggesting that the mechanism by which metformin suppresses these cell divisions is independent of AMPK. Our results showed that the mTOR pathway indeed played a role in controlling germ cell proliferation, but it was not involved in the neuronal Q-cell divisions occurring in L1-arrested worms. We found that the neuronal Q-cells divisions were hold at G1/S cell stage by metforminin vivo. Additionally, we demonstrated that metformin could reduce the phosphorylation activity of BRAF and block the BRAF-MAPK oncogenesis pathway to regulate neuronal Q-cell divisions during L1 arrest. This work discloses a new mechanism by which metformin treatment acts to promote neuronal cancer prevention, and these results will help promote the study of the anticancer mechanisms underlying metformin treatments.
The building blocks of organizational resilience: a review of the empirical literature
Authors: Vakilzadeh Kijan , Haase Alexander 
Published: November 2020

PurposeResilience is critical for organizations in today's volatile business environment, yet some will survive (and even thrive) despite adversity, while others will perish. Why do some organizations handle adversity better than others? The past literature confirms the importance of specific resources, capabilities and structures in dealing with adversity. However, empirical research on organizational resilience remains highly diverse, and the available results have not yet been presented succinctly.Design/methodology/approachA literature review of empirical research on organizational resilience was conducted to summarize the diverse findings of 69 studies, focusing on the factors that lead to resilience.FindingsSeveral building blocks affect how organizations successfully anticipate, cope with and adapt to adversity. Anticipation entails environmental scanning, resilience plans, specific leadership behavior and resources. Coping necessitates particular leadership qualities, a certain organizational culture and innovation. Adaptation requires an organization to learn from adversity and initiate change processes, which influences its ability to anticipate adversity in the long run.Originality/valueBy exclusively analyzing empirical research on organizational resilience, this study summarizes and assembles the results into building blocks for organizational resilience. The findings elaborate on the composition of a concept that is known for its complexity.
Conditional factors for training activities in Chinese, Indian and Mexican subsidiaries of German companies
Authors: Vogelsang Beke , Pilz Matthias 
Published: November 2020

PurposeThe purpose of this paper is to analyse the qualification measures of 12 German multi-national companies (MNCs), all of which are present in China, India and Mexico. In particular, the transfer of dual initial training practices and further training measures are investigated. It examines the impact consistent training strategies across national borders have emerged in German companies or local arrangements have developed despite identical internal influencing factors.Design/methodology/approachBecause of its design, the focus is on the external factors that influence the companies’ training measures. However, an exploratory approach was followed. To pursue the research question face-to-face expert interviews were conducted with 46 training managers in 12 active companies in all 3 countries. The interviews were completely transcribed and evaluated using qualitative methods.FindingsThe analysis shows that it is not internal company factors but country-specific contextual factors that influence training measures and that companies cannot act in the same way worldwide.Research limitations/implicationsThe study is based on 12 MNC and only analyses the blue-collar area. Therefore, it would have to be evaluated whether a similar analysis would result from a survey of other companies in different sectors or whether the differences in terms of training and further training measures would then be even greater.Practical implicationsThe study supports the internationalization strategies of MNC by providing first-hand empirical results concerning recruitment and training of blue colour workers on an intermediate skill level. It gives evidence on the need of national adaptation in the process of transferring training cultures from countries of origin into the host countries. More attention must, therefore, be paid to external factors when developing and implementing training measures.Social implicationsThe economic development in many countries includes an expansion of foreign investments. MNC provides employment and income for workers and their families. However, successful foreign investments also include sustainable recruitment and training strategies of the local workforce. The results of the study support policymakers to guide and support foreign companies to develop successful Human Resource Management strategies in the host countries.Originality/valueThis paper is original because due to the research design the internal factors are kept largely constant and the external influencing factors are singularly focused in detail. Therefore, this procedure makes it possible to investigate whether consistency training strategies across national borders have emerged in German companies or local arrangements have developed despite identical internal influencing factors.
How is HRD related to other disciplines? The analysis of 100 most frequently cited empirical articles published in three HRD journals
Authors: Seo Jaekyo , Lee Suhyung , Ardichvili Alexandre 
Published: November 2020

Purpose
Human resource development (HRD) as an applied discipline is an example of a scholarly field that has emerged through the collaboration of scholars and practitioners with backgrounds in various foundational disciplines. This study interested in both the influence of other disciplines on HRD research and in HRD’s influence on other disciplines. The purpose of this study is to take stock of the relationships between HRD research and research in other academic disciplines affecting and affected by HRD research.

Design/methodology/approach
The study used content analysis of the top 100 most frequently cited empirical articles published in three HRD journals between 2000 and 2019 to identify disciplinary foundations of HRD research. The influence of HRD on other disciplines was scrutinized through citation analysis of work citing the top 100 articles. In addition, categorizing the 100 articles into six research themes, the influence of six research themes on citation patterns was explored.

Findings
The findings indicated that empirical research in HRD, as represented by the top 100 articles, relied mainly on theories and frameworks from two disciplines, management and psychology. Another important finding is that the top 100 HRD articles were cited most often in management publications and the rate of citation in management journals has been growing rapidly since 2005. The citation frequency of HRD articles in other disciplines including psychology, social sciences, education and medicine and nursing shows a general upward trend as well. In addition, there was a difference in disciplines providing theoretical foundations to the HRD articles and citing the articles depending on six predominant research themes, identified in HRD articles.

Originality/value
This study empirically identified theories and disciplines contributing to HRD research, considering the influence of cited research on the HRD articles through content analysis. In addition, the findings of this study broadened the understanding of the relationship between HRD research and other disciplines by examining the contributions of HRD articles to other disciplines. Finally, this research provided new information regarding the changes in dominant themes in HRD research over time.
Women leading change: re-shaping gender in Ghanaian mines
Authors: Kilu Rufai Haruna , Omorede Adesuwa , Uden Maria , Sanda Mohammed-Aminu 
Published: November 2020

Purpose
There is growing attention towards inclusive mining to make an economic case for gender equality and diversity in the industry. Available literature lacks sufficient empirical evidence on the subject matter in Ghana. Therefore, this paper aims to understudy women miners in Ghana and document their role in recent change regimes in mine works gender profiles. An observed change that is stimulating a shift in background dispositions leads to increasing number of women taking up mine jobs.

Design/methodology/approach
In working towards achieving the aim of the study, both qualitative design and a multiple case study approaches are deployed. Four multinational Ghanaian mines and a mining and technology university were used to understudy the women miners and their role towards a change in mine work gender perspectives.

Findings
The results showed a regime of “ore-solidarity movement” (women in mining – Ghana). A kind of solidarity identified conventionally as a social movement in active resource and self-mobilization, engaged in a symbolic contestation for change of the status quo (dominant masculinity cultures) in furtherance of gender equity and inclusion in milieu of mine works reforms in Ghana.

Originality/value
The study is of high scientific, political and public interest to better understand women’s movements in the mining industries in Ghana and to frame them theoretically. It offers solid empirical evidence on roles women miners play to ensure gender shape-shifting and liberalizing the mining space for women’s participation. This move towards inclusive mining implies poverty eradication among women, work towards achieving sustainable mining, competitiveness and assurance for gender-driven social innovative mining.
An economic production model with imperfect quality components and probabilistic lead times
Authors: El-Kassar Abdul-Nasser , Ishizaka Alessio , Temouri Yama , Al Sagheer Abdullah , Vaz Daicy 
Published: November 2020

PurposeThis study investigates a production process that requires N kinds of components for the production of a finished product. The producer orders the various kinds of components from different suppliers and receives the orders in lots at the beginning of each production cycle. Similar to situations often encountered in real life, the lead times are random variables with known probability distributions so that a production cycle starts whenever all N kinds of components become available. Each of the lots received at the start of a production run contains both perfect and imperfect quality components. Once all N kinds of components become available, the producer initiates a screening process to detect the imperfect components. The production of the finished product uses only perfect quality components. The imperfect components are removed from inventory whenever the screening process is completed. The percentage of components of perfect quality present in each lot is a random variable with a known probability distribution.Design/methodology/approachThis production process is described and modeled mathematically and the optimal production/ordering policy is derived based on the mathematical model.FindingsThe formulated mathematical model resulted in the determination of the optimal policy consisting of the optimal number of finished items ordered to be produce during each production run, the number of components ordered from each supplier, and the reorder point. The derived closed form expression for the optimal lot size depends on the minimum of the number of perfect quality components in a lot, whereas the reorder point is determined based on the maximum lead time.Practical implicationsThe modeling approach and results of this study provide practical implications that may be beneficial to both production and supply chain managers as well as researchers.Originality/valueThis modeling approach that incorporates decision-making related to the logistics of acquiring the components and accounts for the probabilistic nature of the lead times and quality of components addresses a gap in the logistics/production literature.
Digitalization opportunities for the procurement function: pathways to maturity
Authors: Seyedghorban Zahra , Samson Danny , Tahernejad Hossein 
Published: November 2020

PurposeThis research aims at investigating the common practical problem of how procurement can be transformed from tactical and administrative to becoming an organizational strategic partner and indeed a competitive weapon, using modern technologies in particular. We investigated how procurement can be reinvented, from being digitized to digitalized to digitally integrated, ultimately contributing in business terms beyond supply chain effectiveness but also to profit generation.Design/methodology/approachA case study approach was designed to investigate three firms, each at very different stages of digital maturity in procurement. Interviews with managers, investigation of processes and documentary materials and in-depth follow-up discussions were conducted.FindingsThe iterative digitalization transformation discovered encompasses complexities rooted in organizational structure, supply chain design and the management of the technology for employees' uptake. There are both operations and strategy implications as a result. This initial research phase led to mapping a model of digital maturity as well as identifying its underlying constructs.Originality/valueThis research discovered that the implementation of digital technologies can lead the procurement function of the supply chain to completely grow out of its administrative and clerical shell into a strategic, consultative, value-adding and potentially revenue-generating function, thereby contributing to the well-being of not only the supply chain but also the entire organization.
Cybersecurity and global regulatory challenges
Authors: Bechara Fabio Ramazzini , Schuch Samara Bueno 
Published: November 2020

Purpose
This study aims to define objectively what are the elements that should be considered in the repositioning of international cooperation, less under its value, which is unquestionable, but more under the optics of the procedure, how can it be operationalized. International cooperation goes beyond the regulatory effort, which, although an important step, is insufficient. It is inserted in an environment in which there is a multiplicity of forces and instances, non-converging and tensioned. At the same time, in the authors’ view, it is not about cooperation between states or between states and international organizations only, it must understand the private sector equally, which has the expressive property of the technologies used.

Design/methodology/approach
The study uses an interdisciplinary approach, and the method of analysis is the typothetical deductive.

Findings
Cybersecurity as a global and complex issue demands cooperation between nations, but also the private sector and civil society engagement. It also demands a good governance in the decision making process, more integrated, accurated and precised.

Originality/value
This study is original, and it represents a special concern and vision from professional and academic fields.
Efficacy of rtsa-byugs vs diclofenac gel in relieving knee pain of patients with osteoarthritis of the knee
Authors: Nidup Dorji , Kietinun Somboon , Niempoog Sunyarn , Sriyakul Kusuma 
Published: November 2020

PurposeRtsa-byugs, a massage oil from Bhutan, is a traditional herbal formula known for its anti-inflammatory properties and used in osteoarthritis treatment. This study investigates the efficacy of rtsa-byugs vs diclofenacgel in relieving knee pain in osteoarthritis patients.Design/methodology/approachA single-blind, randomized controlled trial was conducted amongst osteoarthritis knee patients at an orthopedic outpatient department of Thammasat University Hospital. Participants were randomly allocated to the rtsa-byugs (N = 31) or the Diclofenac gel (N = 31) group. Primary outcomes were assessed by the knee injury and osteoarthritis outcome scores (KOOS), visual analog scale (VAS) and goniometer at day 0, 1, 3, 7.Findings62 participants completed the study. The result of the KOOS scores demonstrated a significant improvement of symptoms at the end of the study in both treatment groups. Improvement of symptoms, pain, daily life living, sport and recreational score and quality of life assessment showed a significant difference from baseline (p < 0.001) within both groups. The quality of life score for the rtsa-byugs group increased significantly on day 3 and 7. The VAS score in both groups decreased with a significant difference from baseline to day 7. The mean value of extension of angle measurement was decreased in day 7, and the mean of flexion score increased in both groups when compared with the baseline.Research limitations/implicationsThe duration of the study was very limited and included a small sample consisting of men and women.Originality/valueRtsa-byugs is safe and effective in relieving pain from osteoarthritis of the knee and can be used as an alternative treatment for knee osteoarthritis.
Employee learning stimulated by manager-as-coach
Authors: Lyons Paul , Bandura Randall 
Published: November 2020

Purpose
The purpose of this paper is to explore the ways a manager in a coaching role may influence employees to embrace a learning orientation based upon the growth mindset. Conceptual in nature, this paper uses recent research, interpretations, explanations and suggestions to propose how manager-as-coach can informally and formally apply basic interventions to assist employee learning and change.

Design/methodology/approach
Based upon a review of relevant literature of theory and practice using several search tools, the authors have isolated a few critical areas to explore to include role of the coach, performance appraisal – the stimulus for manager-as-coach interaction with an employee, feedback orientation and environment, the growth mindset and learning orientation and the dynamics of self-regulated learning. Information from these areas is integrated to inform practitioners of approaches to take in a manager-as-coach role.

Findings
Included for each of the main segments presented are specific, practitioner “Commitment advice/action agendas” for manager-as-coach to stimulate and guide employee learning. These agendas contribute sound, practical information to the body of information concerned with manager-as-coach.

Originality/value
A contribution this work makes is to propose how learning orientation and the growth mindset are intertwined to the extent they assist the manager-as-coach supply the motivational support for employee learning. The resultant learning may lead to one or more of: improved work performance, individual development, attitudes toward learning and increased employee commitment or engagement.