bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cannabidiol and Refractory Epilepsy: Parental and Caregiver Perspectives of Participation in a Compassionate Access Scheme
Authors: Harte Suzanne , Singh Yeeshu , Malone Stephen , Heussler Helen , Wallace Geoffrey 
Published: July 2020

Abstract
BackgroundThe Compassionate Access Scheme (CAS) being delivered through the Queensland Children’s Hospital is designed to allow access to an investigational purified Cannabidiol oral solution to paediatric patients with severe refractory epilepsy.The objectives of this study were to conduct semi-structured interviews to:1. Understand families’ expectations and attitudes about the use of an investigational cannabinoid product for their child’s seizures;2. Understand families’ perceptions of Cannabidiol’s efficacy for their child’s seizures; and other aspects of their child’s behaviour, quality of life and/or cognition.MethodsChildren aged 2-18 years had been enrolled in,orwere enrolled in a compassionate access scheme for Cannabidiol at the time of the study. Semi-structured interviews (n=19) with parents or caregivers (n=23) of children diagnosed with refractory epilepsy were voice-recorded, transcribed and analysed to generate common themes. ResultsKey themes emerged relating to seizure activity, family and school engagement, drug safety and legal access, efficacy, clinical support, social acceptance of the medication and program delivery. The use of Cannabidiol was perceived to have benefits in relation to reducing the severity and frequency of seizure activity for some, but not all patients experiencing refractory epilepsy. For other patients, benefits included improved social engagement, wakefulness and a reduction of side effects related to a reduction of conventional medication dosage.ConclusionThis study provided unique perspectives of families’ experiences managing untreatable epilepsy, their experiences with conventional and experimental pharmacological treatments and health services. Whilst families’ perceptions showed the use of Cannabidiol did not provide a therapeutic reduction in the seizure activity for all patients diagnosed with refractory epilepsy, it’s use as an additional pharmacological agent was perceived to provide other benefits by some patient families.
Collision Minimization Beacon Scheduling Scheme using RPL in Dense TSCH-based IoT Environment
Authors: Kim Jae-Young , Chung Sang-Hwa , Kim Gihwan , Kim Minjae 
Published: July 2020

Abstract
IEEE 802.15.4e Time Slotted Channel Hopping (TSCH) operates on a time-division scheme and uses 16 separate channels for each communication to ensure high reliability even in an industrial environment where many devices are concentrated. The process of participating in the network is indispensable because the entire network operates at the same time. However, there is a problem with TSCH's channel change technique, which results in longer network participation time for new nodes wanting to participate in the network. Previous research has randomly chosen channels for beaconing to reduce network formation time. However, this approach is effective for networks with fewer nodes, but conflicts in beacon messages in networks with approximately 20 or more networks result in network congestion, which increases network participation time. To solve this problem, this paper proposes a collision minimization scheduling technique. This strategy assigns a separate time zone to each node to exclude the possibility of beacon message collisions. This algorithm allocates timeslot based on RANK of RPL. By explicitly predicting the longest network joining time of the network participating nodes, the average network joining time was measured to be about 25 seconds to 27 seconds faster compared to the previous research.
Prevalence of Mild Cognitive Impairment in Community-Dwelling Chinese Populations Aged over 55 years: A Meta-Analysis and Systematic Review
Authors: Lu Yuan , Liu Chaojie , Yu Dehua , Fawkes Sally , Ma Jia , Zhang Min , Li Chunbo 
Published: July 2020

Abstract
Background: Mild cognitive impairment (MCI) is an intermediate phase between normal cognitive ageing and overt dementia, with amnesic MCI (aMCI) being the dominant subtype. This study aims to synthesise the prevalence results of MCI and aMCI in community-dwelling populations in China through a meta-analysis and systematic review.Methods: The study followed the Preferred Reporting Items for Systematic reviews and Meta-Analyses (PRISMA) protocol. English and Chinese studies published before 1 March 2020 were searched from ten electronic bibliographic databases. Two reviewers screened for relevance of the studies against the pre-defined inclusion and exclusion criteria and assessed the quality of the included studies using the Risk of Bias Tool independently. A random-effect model was adopted to estimate the prevalence of MCI and aMCI, followed by subgroup analyses and meta-regression. Sensitivity and publication bias tests were performed to verify the robustness of the meta-analyses.Results: A total of 41 studies with 112,632 participants were included in the meta-analyses. The Chinese community-dwelling populations over 55 years old had a pooled prevalence of 12.2% [95% confidence interval (CI): 10.6%, 14.2%] for MCI and 10.9% [95% CI: 7.7%, 15.4%] for aMCI, respectively. The prevalence of MCI increased with age. The American Psychiatric Association’s Diagnostic tool (DSM-IV) generated the highest MCI prevalence (13.5%), followed by the Petersen criteria (12.9%), and the National Institute on Aging Alzheimer’s Association (NIA-AA) criteria (10.3%). Women, rural residents, and those who lived alone and had low levels of education had higher MCI prevalence than others. ​Conclusion：Higher MCI prevalence was identified in community-dwelling populations in China compared with some other countries, possibly due to more relaxed criteria being adopted for confirming the diagnosis. The study shows that aMCI accounts for 66.5% of MCI, which is consistent with findings of studies undertaken elsewhere.Systematic review registration number： PROSPERO CRD42019134686
Response of Potato to Nitrogen and Phosphorus Fertilizer at Wag-Lasta Areas of Eastern Amhara, Ethiopia
Authors: Sebnie Workat , Esubalew Tilahun , Mengesha Merse 
Published: July 2020

Abstract
Background: Production and productivity of potato in Ethiopia is far below the world average due to soil fertility and other complicated problems. From the production constraints nutrient depletion due to soil erosion is a serious problem. Annually, nitrogen 122 kg ha-1 P 13 kg ha-1and K 82 kg ha-1 was estimated to deplete from Ethiopia (Haileslassie et al., 2005). The essential nutrients like, nitrogen and phosphorus are the most important influential nutrient for production of potato but they are deficient in most Ethiopian soils and thus application of these nutrients could significantly increase crop yields. In this context an experiment was conducted at Sekota and Lasta Lalibela districts (Woleh and Kechin Abeba irrigation command areas) of eastern Amhara to investigate the effects of nitrogen and phosphorus fertilizers for yield and yield component of potato under irrigation condition. Methods: Four rates of nitrogen (0, 46, 92 and 138 Kg N kg ha-1) and four rates of phosphorus (0, 23, 46, and 69 Kg P2O5 kg ha-1) were combined in factorial arrangement and laid out in randomized complete block design with three replications. Results: the result of the study revealed that nitrogen and phosphorus had significant effect on plant height, marketable, and total yield of potato. But phosphorus did not show a significant effect on plant height and unmarketable yield at Sekota district of Woleh irrigation command area. Highest yield 45.55 t ha-1 was obtained from in combined application of 138 N and 23 P2O5 in Lalibela and 17.12 t ha-1 was obtained from in combined application of 138 N and 46 P2O5 from Sekota districts of Woleh irrigation command area. Conclusion: application of nitrogen and phosphorous fertilizer in the study area was more efficient in terms of tuber yield in Lalibela (Kechin Abeba) than Woleh. Cost benefit analysis indicated that application of 46 N kg ha-1 and 23 P2O5 kg ha-1 is required for optimum production of potato in Woleh and application of 138 kg ha-1 N combined with 23 kg ha-1 P2O5 is found to be the appropriate rates for optimum productivity of Potato at Lalibela under irrigation conditions and the same agro-ecology.
Unusual Complications Following Left Ventricular Assisted Device Implantation: Case Series
Authors: Shalabi Amjad , Kachel Erez , Kassif Yigal , Faqeeh Muin , Preisman Sergey , Sternik Leonid , Grosman-Rimon Liza , Ghanem Diab , Amir Offer , Ram Eylon , Lavee Jacob , Grupper Avishai 
Published: July 2020

Abstract
Background:While left ventricular assisted devices (LVAD) have revolutionized the treatment of advanced heart failure, they are associated with a wide range of complications, including bleeding and infection which are the most common complications reported in the literature. Our case series report four unusual complications not related to gastrointestinal bleeding and infections and their management.Case presentation: A sixty one year old female after LVAD implantation with late onset of severe symptomatic aortic regurgitation treated by transfemoral transcatheter valve implantation (TAVI) with good long term results. A Seventy-five year old male patient with acute pump failure secondary to cable damage, who underwent urgent pump replacement. A forty-nine year old female patient with a history of myoma who developed massive uterine bleeding which was treated with emergent open hysterectomy after failed gonadotropin-releasing hormone therapy replacement. A fifty-seven year old male patient with device display failure one month after LVAD implantation without the ability to monitor speed, power consumption and blood flow.Conclusions:The incidence of LVAD complications not related to gastrointestinal bleeding and infections is variable and generally unexpected. It is important to know these complications and their management.
A mink (Neovison vison) model of self injury: effects of CBP-CREB axis on nerve injury and behavior
Authors: Liu Chunxiao , Guo Xiaolan , Si Huazhe , Li Guangyu 
Published: July 2020

Abstract
BackgroundSelf-injurious behavior (SIB) is a clinically challenging problem within both the general population and several clinical disorders. However, the precise molecular mechanism of SIB is still confused, and few animal models exist. Here, we systematic investigated the genesis and development of SIB based on behavioral and pathophysiology study in mink (Neovison vison) models.MethodWe used night-vision video to observe the mink behavior for four weeks,HE stain was performed to characteristic the pathology change of brain. We performed IHC assay to detect the protein level of Iba-1、p-CREB 、CBP and p300 in the brain tissues. Elisa assay used to examined the levels of NfL and NfH in serum and CSF of mink. qRT-PCR assay was mused to detected the expression of Bcl2, NOR1, FoxO4、c-FOS、CBP and p300 in brain tissues. Western blot was used to detect the protein levels of p-CREB、CBP and p300 in brain tissues. We also used Evans Blue as a tracer to detect whether the blood brain barrier was impaired in the brain of mink.RusultFirst we combine behavioral testing, histopathological and molecular biology experiments found that CBP was related with SIB. Mechanism analysis showed that the dysregulation of CBP in brain activated CREB signaling, resulting in nerve damage of brain and SIB symptoms in minks. Importantly, the CBP-CREB interaction inhibitor helped to relieve SIB and nerve damage in brain tissues.ConclusionOur results illustrate an induction of CBP and an activation of CREB, as a novel mechanism in the genesis of SIB. These finding indicate that CBP-CREB axis is critical for SIB and demonstrate the efficacy of the CBP-CREB interaction inhibitor in treating these behaviors.
Molecular dynamics simulation and experimental study of the surface-display of SPA protein via Lpp-OmpA system for screening of IgG
Authors: Vahed Majid , Ramezani Fatemeh , Tafakori Vida , Mirbagheri V.S. , Najafi Akram , Ahmadian Gholamreza 
Published: July 2020

Abstract
Staphylococcal protein A (SpA) is a major bacterial virulence factor of Staphylococcus aureus. S. aureus is capable of escaping from immune system recognition by the surface display of protein A. The SpA protein is broadly used to purify immunoglobulin G (IgG) antibodies. This study investigates the ability of the fusion of Lpp’-OmpA (46 − 159) to anchor and display five repeat domains of protein A with 295 residues length (SpA295) of S. aureus on the Escherichia coli cell surface to develop a novel bioadsorbent. First, the binding between Lpp’-OmpA-SPA295 and IgGFc and anticipation of this structure was investigated using molecular dynamics simulation. Then high IgG recovery from human serum by the surface-displayed system of Lpp’-OmpA-SPA295 performed experimentally. In silico analysis showed the binding potential of SPA295 to IgG after surface expression on LPP-OmpA. Surface-engineered E. coli displaying SpA protein and IgG-binding assay with SDS-PAGE analysis exhibited the high potential of the expressed complex on the surface of E. coli for IgG recapture from human serum which is applicable to conventional immune precipitation.
Integrated Blood Pressure-Chemical Sensing Epidermal Patch
Authors: Sempionatto Juliane , Lin Muyang , Yin Lu , Paz Ernesto de la , Pei Kexin , Sonsa-ard Thitaporn , Silva Andre de Loyola , Khorshed Ahmed , Zhang Fangyu , Tostado Nicholas , Xu Sheng , Wang Joseph 
Published: July 2020

Abstract
The growing demand for tracking the effects of diverse daily activities upon the body physiological response calls for continuous monitoring devices, with integrated hemodynamic and metabolic sensing modalities. This work presents the first demonstration of an integrated wearable sensor that monitors the blood pressure and heart rate via ultrasonic transducers, along with parallel non-invasive electrochemical detection of biomarker levels, such as glucose, lactate, caffeine, and alcohol, in sweat and interstitial fluid. Such simultaneous non-invasive blood-pressure/chemical sensing was implemented by monitoring the dynamic effects of everyday activities, such as exercise and intake of food and drinks, upon the user’s physiological states. Leveraging novel material selection and assembly processes, the multiplexed sensing modalities were optimized to ensure reliable sensing without crosstalk between individual sensors, along with mechanical resiliency and flexibility for conformal contact to curved skin surfaces. The simultaneous acoustic and electrochemical sensors were evaluated on multiple human subjects under different stimuli, and the dynamic correlation of the hemodynamic activities and corresponding metabolic variations was monitored and discussed. Such multimodal blood-pressure/chemical wearable sensor offers a collection of previously unavailable information towards enhancing our understanding of the body’s response to common activities while holding considerable promise for predicting abnormal cardiac events and improving remote, telemetric, and personalized healthcare medical outcome.
Hippocampal spatial and sequential representations of event structure scaffold precise episodic temporal order memory
Authors: Liu Chuqi , Ye Zhifang , Chen Chuansheng , Axmacher Nikolai , Xue Gui 
Published: July 2020

Abstract
The hippocampus plays an important role in representing spatial locations and sequences and for transforming representations via pattern separation and completion. How these representational structures and operations support memory for the temporal order of random items is still poorly understood. We addressed this question by leveraging the method of loci (MOL), a powerful mnemonic strategy for temporal order memory that particularly recruits hippocampus-dependent computations of spatial locations and associations. Applying representational similarity analysis to fMRI activation patterns revealed that hippocampal subfields contained representations of both temporal context and multiple features of sequence structure, including location identity, distance, and sequence boundaries. Critically, the hippocampal CA1 and CA23DG exhibited spatial and sequential pattern separation, respectively, enabling the encoding of multiple items in the same location and reducing swap errors across adjacent locations. Our results suggest that the hippocampus can flexibly reconfigure multiplexed event structure representations to support accurate temporal order memory.
Gut microbiota and fecal metabolites in captive and wild North China leopard (Panthera pardus japonensis) by comparsion using 16s rRNA gene sequencing and LC/MS-based metabolomics
Authors: Cao Heqin , Hua Yan , Wang Jiao , He Fengping , Jiang Guangshun 
Published: July 2020

Abstract
Background: Gut microbes significantly contribute to nutrient digestion and absorption, intestinal health and immunity, and are essential for the survival and environmental adaptation of wild animals. However, there are few studies on the gut microbiota of captive and wild North China leopard (Panthera pardus japonensis). Results: A total of 10 mainly bacterial phyla were identified in the fecal microbiota of North China leopard, Lachnoclostridium (p = 0.003), Peptoclostridium (p = 0.005), Bacteroides (p =0.008), Fusobacterium (p = 0.017) and Collinsella (p = 0.019) were significantly higher than those of wild North China leopard. Distinct differences in the fecal metabolic phenotypes of captive and wild North China leopard were found, such as content of l-methionine, n-acetyl-l-tyrosine, pentadecanoic acid and oleic acid. Differentially abundant gut microbes were associated with fecal metabolites, especially the bacteria in Firmicutes and Bacteroidetes, involved in the metabolism of N-acetyl-L-alanine and D-quinovose.Conclusion: This study reports for the first time the differences in gut microbiota abundance between captive and wild North China leopard, as well as significant differences in fecal metabolic phenotypes between two groups.
Comparative Analysis of Human Microglial Models for Studies of HIV Replication and Pathogenesis
Authors: Rai Mohammad A , Hammonds Jason , Pujato Mario , Mayhew Christopher , Roskin Krishna , Spearman Paul 
Published: July 2020

Abstract
Background: HIV associated neurocognitive disorders cause significant morbidity and mortality despite the advent of highly active antiretroviral therapy. A deeper understanding of fundamental mechanisms underlying HIV infection and pathogenesis in the central nervous system is warranted. Microglia are resident myeloid cells of the brain that are readily infected by HIV and may constitute a CNS reservoir. We evaluated two microglial model cell lines (C20, HMC3) and two sources of primary cell-derived microglia (monocyte-derived microglia [MMG] and induced pluripotent stem cell-derived microglia [iPSC-MG]) as potential model systems for studying HIV-microglia interactions. Results: All four microglial model cells expressed typical myeloid markers with the exception of low or absent CD45 and CD11b expression by C20 and HMC3, and all four expressed the microglia-specific markers P2RY12 and TMEM119. Marked differences were observed upon gene expression profiling, however, indicating that MMG and iPSC-MG cluster closely together with primary human microglial cells, while C20 and HMC3 were similar to each other but very different from primary microglia. Expression of HIV-relevant genes also revealed important differences, with iPSC-MG and MMG expressing relevant genes at levels more closely resembling primary microglia. iPSC-MG and MMG were readily infected with R5-tropic HIV, while C20 and HMC3 lack CD4 and require pseudotyping for infection. Despite many similarities, HIV replication dynamics and HIV-1 particle capture by Siglec-1 differed markedly between the MMG and iPSC-MG. Conclusions: MMG and iPSC-MG appear to be viable microglial models that are susceptible to HIV infection and bear more similarities to authentic microglia than two transformed microglia cell lines. The observed differences in HIV replication and particle capture between MMG and iPSC-MG warrant further study.
Molecular Characterizations, Virulence Determinants and Antimicrobial Resistance Profiles of Methicillin-Resistant Staphylococcus aureus (MRSA) in the North of Iran
Authors: Kouhsari Ebrahim , Hosseini Mojgan , Ahmadi Alireza , Khadivar Parand , Rahimi Somayeh , Amini Abolfazl 
Published: July 2020

Abstract
Background: Emergence and prevalence of Methicillin-Resistant Staphylococcus aureus (MRSA) has become a major universal health concern, limiting therapeutic options. Methods: In the North side of Iran, during the years 2015 to 2017, a total number of 37 MRSA isolates, including 19 clinical isolates from hospitalized patients and 18 colonizing isolates from health care workers were identified from three hospitals, in Gorgan, North of Iran. Antimicrobial susceptibility test was performed using the disk diffusion method and E-test. The presence of virulence and antibiotic resistance determinants were evaluated by PCR. The genotypic characterization was further analyzed using multi-locus sequence, spa, SCCmec, and agr typing.Results: The frequency of MRSA among S. aureus isolates was 38.14% (37/97). The most frequent S. aureus resistant isolates were found to be obstinate against penicillin (98%) and gentamicin (82.5%). Additionally, the lowest resistance rates were found against daptomycin (0%), vancomycin (2.7%), and quinupristin-dalfopristin (5.4%). All MRSA isolates were susceptible to daptomycin with MIC50/MIC90 of 0.25/0.5 µg/ml. One isolate belonging to the ST239-SCCmecIII/t037 clone (MIC≥16μg/ml) was resistant to vancomycin. All but one isolate that shares the ST22-SCCmec IV/t790 strain were positive for both tsst and pvl genes. The most predominant MRSA isolates (27%) were associated with the ST239-SCCmec III/t037 clone; and followed by ST239-SCCmec III/t924 (16.2%).Conclusions: In our study, circulating MRSA strains were genetically diverse with a high prevalence of the ST239-SCCmecIII/t037 clone. These findings emphasize the need for future and continuous surveillance studies on MRSA to prevent the dissemination of multidrug resistance and existing MRSA clones in an effective manner.
The GBA p.G85E mutation in Korean patients with non-neuronopathic Gaucher disease: founder and neuroprotective effects
Authors: Kim Yoo-Mi , Choi Jin-Ho , Kim Gu-Hwan , Sohn Young Bae , Ko Jung-Min , Lee Beom Hee , Cheon Chong Kun , Lim Han Hyuk , Heo Sun-Hee , Yoo Han-Wook 
Published: July 2020

Abstract
Background
Gaucher disease (GD) is caused by a deficiency of β-glucocerebrosidase, encoded by GBA. Haplotype analyses previously demonstrated founder effects for particular GBA mutations in Ashkenazi Jewish and French-Canadian populations. This study aimed to investigate the clinical characteristics and mutation spectrum of GBA in Korean GD patients and to identify founder effect of GBA p.G85E in non-neuronopathic GD patients.
Results
The study cohort included 63 GD patients from 58 unrelated families. Among them, 18 patients from 17 families harboured the p.G85E mutation. Haplotype analysis was performed for 9 probands and their parents for whom DNA samples were available. In 58 unrelated probands, the GBA mutation p.L483P was the most common (30/116 alleles, 26%), followed by p.G85E (16%), p.F252I (13%), and p.R296Q (10%). Of the 18 patients harbouring the p.G85E mutation, their median age at diagnosis was 3.8 (range 1.2–57) years. No patients developed neurological symptoms during follow-up periods of 2.2–20.3 (median 13.9) years. The size of the shared haplotype containing GBA p.G85E was 732 kbp, leading to an estimated age of 3,075 years.
Conclusion
The GBA p.G85E mutation, which appears to be neuroprotective despite producing distinctive visceromegaly and skeletal symptoms, exhibited a potential founder effect in Korean GD patients.