bluuebunny/crossref_metadata_2025_split huggingface dataset:
Two-stage Dynamic Deregulation of Metabolism Improves Process Robustness & Scalability in EngineeredE. coli
Authors: Ye Zhixia , Li Shuai , Hennigan Jennifer N. , Lebeau Juliana , Moreb Eirik A. , Wolf Jacob , Lynch Michael D. 
Published: August 2020

AbstractWe report improved strain and bioprocess robustness as a result of the dynamic deregulation of central metabolism using two-stage dynamic control. Dynamic control is implemented using combinations of CRISPR interference and controlled proteolysis to reduce levels of central metabolic enzymes in the context of a standardized two-stage bioprocesses. Reducing the levels of key enzymes alters metabolite pools resulting in deregulation of the metabolic network. The deregulated network is more robust to environmental conditions improving process robustness, which in turn leads to predictable scalability from high throughput small scale screens to fully instrumented bioreactors as well as to pilot scale production. Additionally, as these two-stage bioprocesses are standardized, a need for traditional process optimization is minimized. Predictive high throughput approaches that translate to larger scales are critical for metabolic engineering programs to truly take advantage of the rapidly increasing throughput and decreasing costs of synthetic biology. In this work we demonstrate that the improved robustness ofE. colistrains engineered for the improved scalability of the important industrial chemicals alanine, citramalate and xylitol, from microtiter plates to pilot reactors.
A JAK/STAT-Mediated Inflammatory Signaling Cascade Drives Oncogenesis In AF10-Rearranged AML
Authors: Chen Bo-Rui , Deshpande Anagha , Barbosa Karina , Kleppe Maria , Li Xue , Yeddula Narayana , Campos Alexandre Rosa , Wechsler-Reya Robert J. , Bagchi Anindya , Meshinchi Soheil , Eaves Connie , Jeremias Irmela , Haferlach Torsten , Frank David A , Ronai Ze’ev , Chanda Sumit , Armstrong Scott A. , Adams Peter D. , Levine Ross L. , Deshpande Aniruddha J. 
Published: August 2020

ABSTRACTLeukemias bearing fusions of the AF10/MLLT10 gene are associated with poor prognosis, and therapies targeting these fusion proteins are lacking. To understand mechanisms underlying AF10 fusion-mediated leukemogenesis, we generated inducible mouse models of AML driven by the most common AF10 fusion proteins, PICALM/CALM-AF10 and KMT2A/MLL-AF10, and performed comprehensive characterization of the disease using transcriptomic, epigenomic, proteomic, and functional genomic approaches. Our studies provide a comprehensive map of gene networks and protein interactors associated with key AF10 fusions involved in leukemia. Specifically, we report that AF10 fusions activate a cascade of JAK/STAT-mediated inflammatory signaling through direct recruitment of JAK1 kinase. Inhibition of the JAK/STAT signaling by geneticJak1deletion or through pharmacological JAK/STAT inhibition elicited potent anti-oncogenic effects in mouse and human models of AF10 fusion AML. Collectively, our study identifies JAK1 as a tractable therapeutic target in AF10-rearranged leukemias.STATEMENT OF SIGNIFICANCEGene fusions of AF10/MLLT10 are recurrent in acute myeloid and lymphoid leukemia and are associated with extremely poor survival outcomes. We show that the JAK1 kinase is required for activation of the AF10 fusion oncotranscriptome and for leukemogenesis. Since a number of JAK/STAT pathways inhibitors are in clinical development or approved for use, our studies may help develop a therapeutic strategy for AF10-rearranged leukemias.
To close or to collapse: the role of charges on membrane stability upon pore formation
Authors: Lira Rafael B. , Leomil Fernanda S.C. , Melo Renan J. , Riske Karin A. , Dimova Rumiana 
Published: August 2020

AbstractResealing of membrane pores is crucial for cell survival. We study membrane surface charge and medium composition as defining regulators of membrane stability. Pores are generated by electric field or detergents. Giant vesicles composed of zwitterionic and negatively charged lipids mixed at varying ratios are subjected to a single strong electric pulse. Charged vesicles are prone to catastrophic collapse transforming them into tubular structures. The spectrum of destabilization responses includes the generation of long-living submicroscopic pores and partial vesicle bursting. The origin of these phenomena is related to the membrane edge tension, which governs pore closure. This edge tension significantly decreases as a function of the fraction of charged lipids. Destabilization of charged vesicles upon pore formation is universal – it is also observed with other poration stimuli. Disruption propensity is enhanced for membranes made of lipids with higher degree of unsaturation. It can be reversed by screening membrane charge in the presence of calcium ions. We interpret the observed findings in light of theories of stability and curvature generation and discuss mechanisms acting in cells to prevent total membrane collapse upon poration. Enhanced membrane stability is crucial for the success of electroporation-based technologies for cancer treatment and gene transfer.
Drug binding sites in the multidrug transporter MdfA in detergent solution and in lipid nanodiscs
Authors: Bahrenberg T. , Yardeni E. H. , Feintuch A. , Bibi E. , Goldfarb D. 
Published: August 2020

AbstractMdfA, a member of the major facilitator superfamily (MFS), is a multidrug/proton antiporter fromE. colithat has been considered a model for secondary multidrug (Mdr) transporters. Its transport mechanism, driven by a proton gradient, is associated with conformational changes, which accompany the recruitment of drugs and their release. In this work, we applied double-electron electron resonance (DEER) spectroscopy to locate the binding site of one of its substrates, tetraphenylphosphonium (TPP) within available crystal structures. We carried out Gd(III)-nitroxide distance measurements between MdfA labeled with a Gd(III) tag and the TPP analog mito-TEMPO (bearing the nitroxide moiety). Data were obtained both for MdfA solubilized in detergent micelles (n-dodecyl-β-D-maltopyranoside (DDM)), and reconstituted into lipid nanodiscs (ND). For both DDM and ND, the average position of the substrate at a neutral pH was found to be close to the ligand position in the If(inward facing) crystal structure, with the DDM environment exhibiting a somewhat better agreement than the ND environment. We therefore conclude that the Ifstructure provides a good description for substrate-bound MdfA in DDM solution, while in ND the structure is slightly modified. A second binding site was found for the ND sample situated at the cytoplasmic side, towards the end of transmembrane helix 7 (TM7). In addition, we used DEER distance measurements on Gd(III) doubly labeled MdfA to track conformational changes within the periplasmic and cytoplasmic sides associated with substrate binding. We detected significant differences in the periplasmic side of MdfA, with the ND featuring a more closed conformation than in DDM, in agreement with earlier reports. The addition of TPP led to a noticeable conformational change in the periplasmic face in ND, attributed to a movement of TM10. This change was not observed in DDM.Statement of SignificanceMdfA is multidrug transporter fromE. coli, which exhibits multidrug efflux activities with an unusually broad spectrum of drug specificities. While it has been established that solute transport by similar transporters is coupled to significant conformational changes, previous studies raised the possibility that this is not the case for MdfA. Moreover, it is not clear how MdfA functionally accommodates chemically dissimilar substrates. Towards resolving these open questions, we used double-electron electron resonance distance measurements to determine the binding site of a spin labeled drug analog within available crystal structures of MdfA and to examine how MdfA responds conformationally to drug binding. Moreover, we explored how these two are affected by the media, detergent micelles vs lipid nanodiscs.
A crossed-disciplinary evaluation of parental perceptions surrounding pediatric non-invasive brain stimulation research
Authors: Behan Michael , Nawshin Tanjila , Nemanich Samuel , Kowalski Jesse , Sutter Ellen , Francis Sunday , Dubinsky Janet , Freese Rebecca , Rudser Kyle , Gillick Bernadette 
Published: August 2020

Purpose
Recruitment for pediatric non-invasive brain stimulation (NIBS) studies is often challenged by low enrollment. Understanding parental perceptions regarding NIBS is crucial to develop new communication strategies to increase enrollment.

Design/methodology/approach
Integrating a crossed-disciplinary approach, the authors conducted a survey at the 2018 Minnesota State Fair querying the perception of risk and preferences of current and future parents associated with pediatric NIBS research. The survey consisted of 28 closed-text questions including demographics, photographs portraying NIBS, terminologies and factors related to NIBS studies.

Findings
Complete surveys were analyzed from 622 parent participants. A significant number of participants (42.8%) perceived the photographs of NIBS as “risky.” Additionally, 65.43% perceived the term “Non-invasive brain therapy” as not risky, a word combination not currently being used when recruiting potential participants. Over 90% (561/622) of participants chose the photograph of child-friendly MRI suite.

Research limitations/implications
Although this survey identified aspects crucial in recruitment for pediatric NIBS research, there were limitations. For example, the authors did not record the sex or demographic distribution (e.g. rural versus urban setting) of the participants. These factors may also influence recruitment messaging.

Originality/value
For important medical research to impact and improve the lives of the potential remedies, participation by the public in clinical trials is necessary. Often the general public perceives the trials as risky as a result of poor marketing communication recruitment material. This study sought to be understood if how the message is encoded has an impact on the decoding by the receiver.
Merchants’ response towards urban tourism development in food markets
Authors: Mashkov Rotem , Shoval Noam 
Published: August 2020

Purpose
In an age when a tourist’s gaze is more involved in the daily lives of locals, it is evident that traditional food markets are being rediscovered as a space for recreation. Yet, the pressure of tourism development may result in retail gentrification to the point of losing the sense of local identity. Focusing on the “boutiquing” process at Mahane Yehuda Market in Jerusalem, this paper aims to measure the physical change in the marketplace, to understand merchants’ attitudes towards tourism development, and to differentiate merchants based on their responses to these changes.

Design/methodology/approach
Two main research methods were used: comparative mapping of the business mix and in-depth interviews with merchants. The first method was used to characterize the physical change in the market, and the second method was used to examine merchants’ attitudes and responses to tourism development.

Findings
There has been a significant physical change in the business mix of the market, with displacing mainly of traditional uses. A strong link between the merchants’ responses to tourism development and their stall ownership status has found.

Research limitations/implications
The limitations of the study lie in its nature as a qualitative study of a case study; there is difficulty in generalizing and drawing universal conclusions.

Originality/value
Add to existing knowledge regarding merchants’ responses in traditional food markets to tourism development in the context of retail gentrification.
Analysis of content strategies of selected brand tweets and its influence on information diffusion
Authors: P Sridevi , Niduthavolu Saikiran , Vedanthachari Lakshmi Narasimhan 
Published: August 2020

PurposeThe purpose of this paper is to design organization message content strategies and analyse their information diffusion on the microblogging website, Twitter.Design/methodology/approachUsing data from 29 brands and 9392 tweets, message strategies on twitter are classified into four strategies. Using content analysis all the tweets are classified into informational strategy, transformational strategy, interactional strategy and promotional strategy. Additionally, the information diffusion for the developed message strategies was explored. Furthermore, message content features such as text readability features, language features, Twitter-specific features, vividness features on information diffusion are analysed across message strategies. Additionally, the interaction between message strategies and message features was carried out.FindingsFinding reveals that informational strategies were the dominant message strategy on Twitter. The influence of text readability features language features, Twitter-specific features, vividness features that influenced information diffusion varied across four message strategies.Originality/valueThis study offers a completely novel way for effectively analysing information diffusion for branded tweets on Twitter and can show a path to both researchers and practitioners for the development of successful social media marketing strategies.
The impact of the COVID-19 lockdown on stock market performance: evidence from Vietnam
Authors: Anh Dao Le Trang , Gan Christopher 
Published: August 2020

PurposeThis study explores the effects of the COVID-19 outbreak and its following lockdown on daily stock returns in Vietnam, a fast-growing emerging market that successfully revived after the pandemic lockdown.Design/methodology/approachThis study uses panel-data regression models to evaluate the influence of the daily increase in the number of COVID-19 confirmed cases during pre-lockdown and lockdown on daily stock returns of 723 listed firms in Vietnam from 30 January to 30 May 2020.FindingsThe study confirms the adverse impact of the daily increasing number of COVID-19 cases on stock returns in Vietnam. The study also discloses that the Vietnam stock market before and during the nationwide lockdown performed in opposing ways. Though COVID-19 pre-lockdown had a significant, negative impact on Vietnam's stock returns, the lockdown period had a significant, positive influence on stock performance of the entire market and the different business sectors in Vietnam. The financial sector was hardest hit on the Vietnam stock market during the COVID-19 outbreak.Research limitations/implicationsThe study indicates investors' confidence and trust in the Vietnam government's decisions to combat COVID-19 and favorable stocks prices were the main reasons that the Vietnam stock market rebounded during and after lockdown.Originality/valueThis is the first study to examine the impact of COVID-19 during the pre-lockdown and lockdown periods on stock performance in Vietnam, a rapidly developing economy that was successful in controlling the pandemic with a rejuvenated stock market after lockdown.
Risk management and corporate governance failures in Islamic banks: a case study
Authors: Alhammadi Salah , Archer Simon , Asutay Mehmet 
Published: August 2020

Purpose
The purpose of this paper is to show how the choice and ongoing evaluation of a firm’s business model, as a matter of strategic guidance, are key aspects of corporate governance (CG), with particular reference to risk management (RM) in Islamic banks.

Design/methodology/approach
This research uses a case study approach, with a single case, which was chosen as it fits very well the purpose of this research. The data collection was based largely on documentary evidence. Company data were collected from company annual reports, press releases and legitimate web sites. The ORBIS Bank Focus database was also used to produce a comparative financial analysis.

Findings
The study findings illustrate how an apparently successful business model may fail due to an inherent instability that could have been identified through the application of careful risk analysis (including stress testing) in the choice and ongoing evaluation of the business model, which robust CG and strategic guidance require. In particular, Arcapita’s problems illustrate the dangers to Islamic financial institutions (IFI) from business models that involve undue exposure to liquidity risk.

Practical implications
The issues raised in the paper are important in that Islamic banking and finance is an integral part of the global banking and finance industry. Investors and regulators are now requesting corporate management to provide improved service to shareholders and other stakeholders alike. IFI rely on the confidence of investors and market participants, just like conventional institutions and when this confidence erodes, it may prove difficult to regain.

Social implications
The global credit crisis of 2008 caused significant difficulties to firms, especially financial institutions, even with substantial government intervention in the economy, which raised some issues of CG and ethics.

Originality/value
This paper extends the knowledge of the potential effects of weaknesses in CG and RM, with specific reference to strategic guidance in the choice and ongoing evaluation of a firm’s business model, especially in relation to the Islamic banking sector. It also provides a telling illustration of the need for the enhancements of the Basel Committee’s prudential requirements set out in the various Basel III documents.
Process mining approach to formal business process modelling and verification: a case study
Authors: Ito Sohei , Vymětal Dominik , Šperka Roman 
Published: August 2020

Purpose
The need for assuring correctness of business processes in enterprises is widely recognised in terms of business process re-engineering and improvement. Formal methods are a promising approach to this issue. The challenge in business process verification is to create a formal model that is well-aligned to the reality. Process mining is a well-known technique to discover a model of a process based on facts. However, no studies exist that apply it to formal verification. This study aims to propose a methodology for formal business process verification by means of process mining, and attempts to clarify the challenges and necessary technologies in this approach using a case study.

Design/methodology/approach
A trading company simulation model is used as a case study. A workflow model is discovered from an event log produced by a simulation tool and manually complemented to a formal model. Correctness requirements of both domain-dependent and domain-independent types of the model are checked by means of model-checking.

Findings
For business process verification with both domain-dependent and domain-independent correctness requirements, more advanced process mining techniques that discover data-related aspects of processes are desirable. The choice of a formal modelling language is also crucial. It depends on the correctness requirements and the characteristics of the business process.

Originality/value
Formal verification of business processes starting with creating its formal model is quite new. Furthermore, domain-dependent and domain-independent correctness properties are considered in the same framework, which is also new. This study revealed necessary technologies for this approach with process mining.
Street vending: transformative entrepreneurship for individual and collective well-being
Authors: Giraldo Mario , Garcia-Tello Luis , Rayburn Steven William 
Published: August 2020

Purpose
This study aims to explore the lived experience of vendors as they enact street vending practice that emerges as transformative entrepreneurship and service where they live and work.

Design/methodology/approach
This research qualitatively explores street vending in a multi-cultural, multi-local study to understand how these businesses operate to positively impact individual, collective and societal well-being.

Findings
This research reveals street vending is a creative, transformative entrepreneurial activity that improves individual and collective well-being. The research exposes multiple forms of habitual and transformative value delivered by vendors, resulting in improved eudaimonic and hedonic well-being that ripples out from vendors to families, communities and society.

Research limitations/implications
A framework of street vending practice is provided to guide service designers and policymakers as they seek to support street vendors as they move from informal to formal and from survival to growth business modes.

Originality/value
This research extends existing conceptualizations of transformative entrepreneurship beyond prior focus on economic transformation and prior limitations of transformative entrepreneurship to business in growth modes.
Older consumers’ reaction to a patronizing sales interaction
Authors: Chéron Emmanuel , Weins Christian , Kohlbacher Florian 
Published: August 2020

Purpose
The purpose of this study is to investigate the impact of patronizing by salespeople when interacting with older consumers in a retail situation of shopping for a mobile phone. In addition to patronizing behavior, the impact of the age of the salesperson and gender of the consumer are explored.

Design/methodology/approach
The study is based on statistical analyzes of a between-participants controlled experiment collected via an online survey of 338 members of the German Senior Citizens League.

Findings
The study contributes to the field of services marketing by confirming that older consumers reject patronizing sales interactions and by showing that men are more tolerant of condescendence than women, especially when younger salespeople are involved.

Research limitations/implications
A limitation of this study is the use of fictional situations with a selected number of manipulated variables in a simulated sales interaction.

Practical implications
Rejection of a patronizing sales interaction was found to be similar by both genders with an older salesperson. Furthermore, retail shops of technical appliances could prevent potential problems by being cautious of having younger male salespeople interacting with older women customers.

Originality/value
Research on the impact of condescending sales interaction as perceived by older consumers is scarce and has not previously considered the role of customer gender and salesperson age. Beyond investigating the perception of participants to patronizing, the role of the salesperson age and customer gender were investigated.
Identification of study habits and skills associated with the academic performance of Bachelor's degree Food Science and Technology students at the University of León (Spain)
Authors: Renes E. , Prieto B. , López M. , Fresno J. M. , González‐Raurich M. , Tornadijo M. E. , Prieto M. , Álvarez‐Ordóñez A. 
Published: August 2020

AbstractThe aim of this study was to identify factors that influence the academic success or failure of Food Science and Technology students at the University of León (Spain). A questionnaire of 68 questions was distributed among 185 students during the 2017 to 2018 academic year. Those students who were satisfied with passing exams, studied in the library, and/or faced personal problems showed low academic performance. In addition, some factors, such as studying Physics previously, attending class, and/or studying all the topics for the exams, were associated (P < 0.05) positively with the grade point average obtained. Academic performance of the students in the different years of study did not present significant differences (P ≥ 0.05). This study reinforces the need to introduce changes in the current University of León´s education program.