bluuebunny/crossref_metadata_2025_split huggingface dataset:
Brugada syndrome genetics is associated with phenotype severity
Authors: Ciconte Giuseppe , Monasky Michelle M , Santinelli Vincenzo , Micaglio Emanuele , Vicedomini Gabriele , Anastasia Luigi , Negro Gabriele , Borrelli Valeria , Giannelli Luigi , Santini Francesca , de Innocentiis Carlo , Rondine Roberto , Locati Emanuela T , Bernardini Andrea , Mazza Beniamino C , Mecarocci Valerio , Ćalović Žarko , Ghiroldi Andrea , D’Imperio Sara , Benedetti Sara , Di Resta Chiara , Rivolta Ilaria , Casari Giorgio , Petretto Enrico , Pappone Carlo 
Published: November 2020

Abstract

Aims
Brugada syndrome (BrS) is associated with an increased risk of sudden cardiac death due to ventricular tachycardia/fibrillation (VT/VF) in young, otherwise healthy individuals. Despite SCN5A being the most commonly known mutated gene to date, the genotype–phenotype relationship is poorly understood and remains uncertain. This study aimed to elucidate the genotype–phenotype correlation in BrS.

Methods and results
Brugada syndrome probands deemed at high risk of future arrhythmic events underwent genetic testing and phenotype characterization by the means of epicardial arrhythmogenic substrate (AS) mapping, and were divided into two groups according to the presence or absence of SCN5A mutation. Two-hundred probands (160 males, 80%; mean age 42.6 ± 12.2 years) were included in this study. Patients harbouring SCN5A mutations exhibited a spontaneous type 1 pattern and experienced aborted cardiac arrest or spontaneous VT/VF more frequently than the other subjects. SCN5A-positive patients exhibited a larger epicardial AS area, more prolonged electrograms and more frequently observed non-invasive late potentials. The presence of an SCN5A mutation explained &gt;26% of the variation in the epicardial AS area and was the strongest predictor of a large epicardial area.

Conclusion
In BrS, the genetic background is the main determinant for the extent of the electrophysiological abnormalities. SCN5A mutation carriers exhibit more pronounced epicardial electrical abnormalities and a more aggressive clinical presentation. These results contribute to the understanding of the genetic determinants of the BrS phenotypic expression and provide possible explanations for the varying degrees of disease expression.
Acuity Differences Among Newly Admitted Older Residents in Rural and Urban Nursing Homes
Authors: Jonk Yvonne , Thayer Deborah , Mauney Karen , Croll Zachariah , McGuire Catherine , Coburn Andrew F 
Published: November 2020

Abstract

Background and Objectives
Our primary objective was to assess rural–urban acuity differences among newly admitted older nursing home residents.

Research Design and Methods
Data included the 2015 Minimum Data Set v3.0, the Area Health Resources File, the Provider of Services File, and Rural–Urban Commuting Area codes. Activities of daily living, the Cognitive Function Scale, and aggression/wandering indicators were used to assess functional, cognitive, and behavioral status, respectively. Excluding assessments for short stays (less than 90 days), assessments for 209,719 newly admitted long-stay residents aged 65 and older across 14,834 facilities in 47 states were evaluated. Difference in differences (DID) generalized linear models with state-fixed effects and clustering by facilities were used to assess the interaction effect of older age (75 plus) on rural–urban acuity differences, controlling for socioeconomic factors, admission source, and market characteristics.

Results
Residents admitted to rural facilities were less functionally impaired (incidence rate ratio: 0.973–0.898) but had more cognitive (odds ratio [OR]: 1.03–1.22) and problem behaviors (OR: 1.19–1.48) than urban. Although older age was predictive of higher acuity, in DID models, the expected decline in functional status was comparable in rural and urban facilities, while the cognitive and behavioral status for older admissions was 8.0% and 8.5% lower in rural versus urban facilities, respectively.

Discussion and Implications
Although the higher prevalence of cognitive impairment and problem behaviors among rural admissions was attributable in part to older age, rural facilities admitted less complex individuals among older age residents than urban facilities. Findings may reflect less capacity to manage older, complex individuals in rural facilities.
Stress drop–magnitude dependence of acoustic emissions during laboratory stick-slip
Authors: Blanke Aglaja , Kwiatek Grzegorz , Goebel Thomas H W , Bohnhoff Marco , Dresen Georg 
Published: November 2020

SUMMARYEarthquake source parameters such as seismic stress drop and corner frequency are observed to vary widely, leading to persistent discussion on potential scaling of stress drop and event size. Physical mechanisms that govern stress drop variations are difficult to evaluate in nature and are more readily studied in controlled laboratory experiments. We perform two stick-slip experiments on fractured (rough) and cut (smooth) Westerly granite samples to explore fault roughness effects on acoustic emission (AE) source parameters. We separate large stick-slip events that generally saturate the seismic recording system from populations of smaller AE events which are sensitive to fault stresses prior to slip. AE event populations show many similarities to natural seismicity and may be interpreted as laboratory equivalent of natural microseismic events. We then compare the temporal evolution of mechanical data such as measured stress release during slip to temporal changes in stress drops derived from AEs using the spectral ratio technique. We report on two primary observations: (1) In contrast to most case studies for natural earthquakes, we observe a strong increase in seismic stress drop with AE size. (2) The scaling of stress drop with magnitude is governed by fault roughness, whereby the rough fault shows a more rapid increase of the stress drop–magnitude relation with progressing large stick-slip events than the smooth fault. The overall range of AE sizes on the rough surface is influenced by both the average grain size and the width of the fault core. The magnitudes of the smallest AE events on smooth faults may also be governed by grain size. However, AEs significantly grow beyond peak roughness and the width of the fault core. Our laboratory tests highlight that source parameters vary substantially in the presence of fault zone heterogeneity (i.e. roughness and narrow grain size distribution), which may affect seismic energy partitioning and static stress drops of small and large AE events.
Anatomical segmentectomy versus pulmonary lobectomy for stage I non-small-cell lung cancer: patients selection and outcomes from the European Society of Thoracic Surgeons database analysis
Authors: Tosi Davide , Nosotti Mario , Bonitta Gianluca , Mendogni Paolo , Bertolaccini Luca , Spaggiari Lorenzo , Brunelli Alex , Ruffini Enrico , Falcoz Pierre Emmanuel 
Published: November 2020

Abstract

OBJECTIVES
The aims of this study were to describe the potential selection criteria for patients scheduled for lobectomy versus segmentectomy for stage I non-small-cell lung cancer and to compare the 2 procedures in terms of intraoperative variables and postoperative outcomes using the European Society of Thoracic Surgeons (ESTS) Registry.

METHODS
This observational multicentre retrospective cross-sectional study was based on data collected from the ESTS database. The following were set as inclusion criteria: pulmonary lobectomy or segmentectomy for stage I primary lung cancer (according to 8th TNM edition), no previous lung surgery and no induction chemotherapy or radiotherapy. Statistical significance was examined using Mann–Whitney or 2 proportions Z tests.

RESULTS
Among 63 542 patients enrolled in the ESTS database (2007–2018), 17 692 met the inclusion criteria: 15 845 patients received lobectomy and 1847 segmentectomy. Video-assisted thoracic surgery (VATS) lobectomy and VATS segmentectomy were the 27.8% and 31.9% of the procedures, respectively. Lobectomy group was significantly younger and had a lower American Society of Anaesthesiology (ASA) score, lower comorbidities prevalence and better respiratory function. The segmentectomy group had lower complications rate (25.6% vs 33.8%). When considering only the last 5 years, ASA score was similar between the 2 groups, although pulmonary function remained significantly lower in the segmentectomy group.

CONCLUSIONS
According to the ESTS database, segmentectomy was preferably offered to ‘compromised’ patients, with limited respiratory function, higher ASA score and relevant comorbidities. Nevertheless, the procedure showed lower complications rate and similar short-term outcomes compared to lobectomy. During the last 5 years, segmentectomy appeared to be regarded as a valid alternative, even for selected patients who could tolerate both procedures.
Monogamy in a Moment: How do Brief Social Interactions Change Over Time in Pair-Bonded Zebra Finches (Taeniopygia guttata)?
Authors: Prior Nora H , Smith Edward , Dooling Robert J , Ball Gregory F 
Published: November 2020

SynopsisResearch on monogamy has largely focused on marked behaviors that are unique to pair bonded partners. However, these marked behaviors represent only a subset of the pair-directed behaviors that partners engage in; the influence of pair bonding on mundane or subtle social interactions among partners remains largely unknown. In this study, we describe the changes that occur during brief social reunions (or greets) over the course of pair bonding in zebra finches. We quantified pair-directed behavior during 5-min reunions from three stages of pair bonding: initial pairing (between 4 and 72 h), early pairing (1–2 weeks), and late pairing (&gt;1 month). These social interactions were operationalized in multiple ways. First, we quantified the overall activity levels (call and movement rates) for both the male and female. Overall, females were more active than males, but for both males and females calling activity was highest at initial pairing. We quantified behavioral coordination between partners in two ways: (1) similarity in call and movement rates between partners and (2) temporal synchrony of calls and movements between partners (via sliding correlation coefficients of time-stamped calls and movements). Overall, there were no effects of pairing stage on behavioral coordination. Finally, we used principal component analyses to disentangle behavioral coordination from the activity levels of the male and female. These results contribute to a growing line of evidence that male and female zebra finches differentially contribute to social dynamics and highlight the influence of pair bonding on the development of social dynamics. Furthermore, our preliminary analyses raise the hypothesis that behavioral coordination during the earliest phases of pairing is modulated by the extent and nature of prior experience. Overall, while behavioral coordination is clearly important for many salient interactions such as duetting, courtship displays, and biparental care, the significance of mundane social interactions for monogamous partnerships remains largely unknown.
The Presence and Accessibility of Competitive Resources Affect Trapping Efficiency of Spotted-Wing Drosophila (Diptera: Drosophilidae)
Authors: Singh Sandeep , Huang Juan , Grieshop Matthew J 
Published: November 2020

Abstract
Spotted-wing drosophila (Drosophila suzukii, (Matsumura)) is an invasive vinegar fly that has become a serious threat to soft fruit crops. Monitoring for this pest is typically performed using drowning traps baited with live yeast cultures or fermentation volatile blends. Trapping programs using these compounds provide highly variable results across production systems, geographic regions, and growing seasons. Trap competition with fruit is one hypothesis for this inconsistency. This study evaluated the trapping efficiency of yeast and wine baits in the presence and absence of small quantities of host fruits in two binary-choice laboratory experiments. The first experiment evaluated trap capture in clear 946-ml traps with easily accessible water, apple pomace, blueberry, raspberry, strawberry, cherry, or grape as competitive influences. The second experiment evaluated the same competitors, but they were made less accessible. Recapture of flies in arenas containing competitive fruit was reduced by 64–88% when fruit was ‘accessible’ and from 0 to 51% when it was ‘inaccessible’ compared with arenas containing a water competitor. All fruit types provided statistically similar levels of trap interference. In the first experiment, yeast captured more flies compared with wine, whereas in the second experiment, wine captured more flies than yeast. Our results support the hypothesis that the presence of fruit or other reproductive resources will reduce trap captures and that this reduction is likely mediated by the relative accessibility of the fruit versus the trap. Thus, attempts to develop population estimates based on traps should incorporate fruit availability/accessibility.
Understanding Pediatric Drug Lag Time: Review of Selected Drug Package Inserts
Authors: Beleck Aviva , Nachman Sharon 
Published: November 2020

Abstract
The wait for a pharmaceutical drug to become approved by the FDA for pediatrics lasts approximately 8 years longer than that for adults. One of the reasons given is the concern that simultaneous pediatric and adult trials may affect licensing in adults. We reviewed drug package inserts obtained from the FDA database for 5 selected agents for the years prior to and after being FDA approved for pediatric use. There were no new contraindications, warnings, or adverse events identified during pediatric clinical trials that would have put adult licensure at risk if approval was obtained in parallel for pediatric populations. The few changes in the package inserts in those years were due to ongoing adult clinical trials and post-marking experience in adults. The concern that pediatric trials might affect adult licensure does not appear to be justifiable.
Scaling parameter of the lethal effect of mammalian cells based on radiation-induced OH radicals: effectiveness of direct action in radiation therapy
Authors: Kusumoto Tamon , Ogawara Ryo , Igawa Kazuyo , Baba Kentaro , Konishi Teruaki , Furusawa Yoshiya , Kodaira Satoshi 
Published: November 2020

ABSTRACT
We have been studying the effectiveness of direct action, which induces clustered DNA damage leading to cell killing, relative to indirect action. Here a new criterion Direct Ation-Based Biological Effectiveness (DABBLE) is proposed to understand the contribution of direct action for cell killing induced by C ions. DABBLE is defined as the ratio of direct action to indirect action. To derive this ratio, we describe survival curves of mammalian cells as a function of the number of OH radicals produced 1 ps and 100 ns after irradiation, instead of the absorbed dose. By comparing values on the vertical axis of the survival curves at a certain number of OH radicals produced, we successfully discriminate the contribution of direct action induced by C ions from that of indirect action. DABBLE increases monotonically with increasing linear energy transfer (LET) up to 140 keV/μm and then drops, when the survival curves are described by the number of OH radicals 1 ps after irradiation. The trend of DABBLE is in agreement with that of relative biological effectiveness (RBE) of indirect action. In comparison, the value of DABBLE increases monotonically with LET, when the survival curves are described by the number of OH radicals 100 ns after irradiation. This finding implies that the effectiveness of C ion therapy for cancer depends on the contribution of direct action and we can follow the contribution of direct action over time in the chemical phase.
The WRKY transcription factor AaGSW2 promotes glandular trichome initiation in Artemisia annua
Authors: Xie Lihui , Yan Tingxiang , Li Ling , Chen Minghui , Ma Yanan , Hao Xiaolong , Fu Xueqing , Shen Qian , Huang Yiwen , Qin Wei , Liu Hang , Chen Tiantian , Hassani Danial , Kayani Sadaf-llyas , Rose Jocelyn K C , Tang Kexuan 
Published: November 2020

Abstract
Glandular secreting trichomes (GSTs) synthesize and secrete large quantities of secondary metabolites, some of which have well-established commercial value. An example is the anti-malarial compound artemisinin, which is synthesized in the GSTs of Artemisia annua. Accordingly, there is considerable interest in understanding the processes that regulate GST density as a strategy to increase artemisinin production. In this study, we identified a GST-specific WRKY transcription factor from A. annua, AaGSW2, which is positively regulated by the direct binding of the homeodomain proteins AaHD1 and AaHD8 to the L1-box of the AaGSW2 promoter. Overexpression of AaGSW2 in A. annua significantly increased GST density, while AaGSW2 knockdown lines showed impaired GST initiation. Ectopic expression of AaGSW2 homologs from two mint cultivars, Mentha spicata and Mentha haplocalyx, in A. annua also induced GST formation. These results reveal a molecular mechanism involving homeodomain and WRKY proteins that controls glandular trichome initiation, at least part of which is shared by A. annua and mint.
An Analysis of Exertional Safety After Blood Donation in Active Duty Military Personnel—A Feasibility Study
Authors: Carius Brandon M , Umar Mohamad , Aden James K , Schauer Steve G 
Published: November 2020

ABSTRACT

Introduction
Whole blood (WB) is the optimal resuscitation fluid in hemorrhagic shock. Military research focuses on mortality benefits of WB acquired through walking blood banks (WBBs). Few military-based studies on donation effects exist, almost exclusively performed on small special operation forces. No Department of Defense regulations for postdonation precautions in nonaviation crew members exist. Further study is warranted regarding safety and limitations in postdonation populations.

Materials and Methods
A feasibility (n = 25) prospective interventional study examined the safety of exertion (defined as a 1.6-km treadmill run at volunteers’ minimum passing pace for the Army Physical Fitness Test) following 1 unit of WB donation. Subjects served as their own controls, performing baseline testing 7 days before donation, with repeat testing 1 h following donation conducted by Armed Services Blood Program personnel. Adverse events, pre- and postexertion vital signs (VS) were evaluated.

Results
There were no adverse events throughout testing. Only resting heart rate (68 vs. 73 beats · min−1, p &lt; 0.01) and postexertion heart rate were significantly different among pre- and postdonation VS. Additional significant findings were time to attain postexertion normocardia (116 vs. 147 seconds, p &lt; 0.01). A small but statistically significant change in Borg perceived exertional scores was noted (10.3 vs. 10.8, p &lt; 0.05).

Conclusions
This feasibility study demonstrates the first safety test of regular military populations performing exertion immediately following the standardized WB donation. VS changes may translate into a small but significant increase in perceived postdonation exertion. Future studies should expand duration and intensity of exertion to match combat conditions.
How Common Knowledge Is Possible
Authors: Immerman Daniel 
Published: November 2020

Abstract
The two of us commonly know a proposition just in case (i) we both know it, (ii) we both know that we both know it, (iii) we both know that we both know that we both know it, and so on. In a recent paper titled ‘Uncommon Knowledge’, Harvey Lederman (2018) argues against the possibility of common knowledge. His argument rests on the empirical claim that there are minor individual variations in how we perceive things. This motivates a principle about perception: when two people are perceiving something and it perceptually appears a certain way to one of them, then for all that person knows, it perceptually appears a slightly different way to the other. In this paper, I challenge Lederman’s perceptual principle and thereby his argument. In particular, I argue that there are some circumstances in which things perceptually appear a certain way to me and nonetheless I know that they don’t perceptually appear in a slightly different way to you. Indeed, I argue that not only are there exceptions to the perceptual principle, but they are widespread.
The exomoon corridor: Half of all exomoons exhibit TTV frequencies within a narrow window due to aliasing
Authors: Kipping David 
Published: November 2020

ABSTRACTExomoons are expected to produce potentially detectable transit timing variations (TTVs) upon their parent planet. Unfortunately, distinguishing moon-induced TTVs from other sources, in particular planet–planet interactions, has severely impeded its usefulness as a tool for identifying exomoon candidates. A key feature of exomoon TTVs is that they will always be undersampled, due to the simple fact that we can only observe the TTVs once per transit/planetary period. We show that it is possible to analytically express the aliased TTV periodicity as a function of planet and moon period. Further, we show that inverting an aliased TTV period back to a true moon period is fraught with hundreds of harmonic modes. However, a unique aspect of these TTV aliases is that they are predicted to occur at consistently short periods, irrespective of what model one assumes for the underlying moon population. Specifically, 50 per cent of all exomoons are expected to induce TTVs with a period between 2 to 4 cycles, a range that planet–planet interactions rarely manifest at. This provides an exciting and simple tool for quickly identifying exomoons candidates and brings the TTV method back to the fore as an exomoon hunting strategy. Applying this method to the candidate, Kepler-1625b i, reveals that its TTV periodicity centres around the median period expected for exomoons.
Characterizing Transcriptional Regulatory Sequences in Coronaviruses and Their Role in Recombination
Authors: Yang Yiyan , Yan Wei , Hall A Brantley , Jiang Xiaofang 
Published: November 2020

Abstract
Novel coronaviruses, including SARS-CoV-2, SARS, and MERS, often originate from recombination events. The mechanism of recombination in RNA viruses is template switching. Coronavirus transcription also involves template switching at specific regions, called transcriptional regulatory sequences (TRS). It is hypothesized but not yet verified that TRS sites are prone to recombination events. Here, we developed a tool called SuPER to systematically identify TRS in coronavirus genomes and then investigated whether recombination is more common at TRS. We ran SuPER on 506 coronavirus genomes and identified 465 TRS-L and 3,509 TRS-B. We found that the TRS-L core sequence (CS) and the secondary structure of the leader sequence are generally conserved within coronavirus genera but different between genera. By examining the location of recombination breakpoints with respect to TRS-B CS, we observed that recombination hotspots are more frequently colocated with TRS-B sites than expected.