bluuebunny/crossref_metadata_2025_split huggingface dataset:
Two co‐inherited novel SNPs in the MC4R gene related to live body weight and hormonal assays in Awassi and Arabi sheep breeds of Iraq
Authors: Al‐Thuwaini Tahreer M. , Al‐Shuhaib Mohammed Baqur S. , Lepretre Frederic , Dawud Halla Hassan 
Published: December 2020

AbstractMelanocortin‐4 receptor (MC4R) gene plays a key role in the regulation of body weight and energy homeostasis. This study aims to evaluate the association of single nucleotide polymorphisms (SNPs) of the MC4R gene with live body weight and hormonal assays in two breeds of sheep that differ in productive performance, Awassi and Arabi. All known coding sequences of the MC4R gene were covered in this study. DNA samples from 150 animals (Awassi and Arabi breed) were genotyped by PCR‐single‐strand conformation polymorphism (PCR‐SSCP) to assess their pattern of genetic variation. Concerning exon 1, clear heterogeneity was detected with three different SSCP‐banding patterns. The sequencing reactions confirmed these variations by detecting the presence of the two novel SNPs, 107G/C and 138A/C, and three genotypes, GC, AC and AA. The 107G/C SNP was detected in GC genotype, while the 138A/C was detected on both GC and AC genotypes. The other SSCP‐banding pattern (AA genotype) did not show any detectable unique variation. Both SNPs were closely and strongly linked in both breeds (D' and r2 values were 1.00), which signifies that both loci were co‐inherited as one unit. Association analysis indicated that both breeds with GC/AC haplotype showed higher live body weight (37.250 ± 0.790) relative to the GG/AA (30.244 ± 0.968) and CC/CC (47.231 ± 1.230) haplotypes (p < .05). Concerning the genotyping of exon 2, only 362 bp showed heterogeneity with a missense mutation, with no significant association (p > .05) with the measured traits. In conclusion, the two novel SNPs (107G/C and 138 A/C) were highly associated with live body weight in both breeds. Haplotype analysis confirmed that these two novel SNPs were in strong linkage disequilibrium (LD) and could be used as genetic markers for sheep phenotypic trait improvement.
Thermal comfort in winter incorporating solar radiation effects at high altitudes and performance of improved passive solar design—Case of Lhasa
Authors: Huang Lingjiang , Kang Jian 
Published: December 2020

AbstractThe solar incidence on an indoor environment and its occupants has significant impacts on indoor thermal comfort. It can bring favorable passive solar heating and can result in undesired overheating (even in winter). This problem becomes more critical for high altitudes with high intensity of solar irradiance, while received limited attention. In this study, we explored the specific overheating and rising thermal discomfort in winter in Lhasa as a typical location of a cold climate at high altitudes. First, we evaluated the thermal comfort incorporating solar radiation effect in winter by field measurements. Subsequently, we investigated local occupant adaptive responses (considering the impact of direct solar irradiance). This was followed by a simulation study of assessment of annual based thermal comfort and the effect on energy-saving potential by current solar adjustment. Finally, we discussed winter shading design for high altitudes for both solar shading and passive solar use at high altitudes, and evaluated thermal mass shading with solar louvers in terms of indoor environment control. The results reveal that considerable indoor overheating occurs during the whole winter season instead of summer in Lhasa, with over two-thirds of daytime beyond the comfort range. Further, various adaptive behaviors are adopted by occupants in response to overheating due to the solar radiation. Moreover, it is found that the energy-saving potential might be overestimated by 1.9 times with current window to wall ratio requirements in local design standards and building codes due to the thermal adaption by drawing curtains. The developed thermal mass shading is efficient in achieving an improved indoor thermal environment by reducing overheating time to an average of 62.2% during the winter and a corresponding increase of comfort time.
Preoperative Frailty Assessment, Operative Severity Score, and Early Postoperative Loss of Independence in Surgical Patients Age 65 Years or Older
Authors: Owodunni Oluwafemi P. , Mostales Joshua C. , Qin Caroline Xu , Gabre-Kidan Alodia , Magnuson Thomas , Gearhart Susan L. 
Published: December 2020

BACKGROUND:
Preoperative discussions around postoperative discharge planning have been amplified by the COVID pandemic. We wished to determine whether our preoperative frailty screen would predict postoperative loss of independence (LOI).

STUDY DESIGN:
This single-institutional study included demographic, procedural, and outcomes data from patients 65 years or older who underwent frailty screening before a surgical procedure. Frailty was assessed using the Edmonton Frail Scale. The Operative Severity Score was used to categorize procedures. The Hierarchical Condition Category risk-adjustment score, as calculated by the Centers for Medicare and Medicaid Services, was included. LOI was defined as an increase in support outside of the home after discharge. Univariable, multivariable logistic regressions, and adjusted postestimation analyses for predictive probabilities of best fit were performed.

RESULTS:
Five hundred and thirty-five patients met inclusion criteria and LOI was seen in 38 patients (7%). Patients with LOI were older, had a lower BMI, a higher Edmonton Frail Scale score (7 vs 3.0; p < 0.001), and a higher Hierarchical Condition Category score than patients without LOI. Being frail and undergoing a procedure with an Operative Severity Score of 3 or higher was independently associated with an increased risk of LOI. In addition, social dependency, depression, and limited mobility were associated with an increased risk for LOI. On multivariable modeling, frailty status, undergoing an operation with an Operative Severity Score of 3 or higher, and having a Hierarchical Condition Category score ≥1 were the most predictive of LOI (odds ratio 12.72; 95% CI, 12.04 to 13.44; p < 0.001). In addition, self-reported depression, weight loss, and limited mobility were associated with a nearly 11-fold increased risk of postoperative LOI.

CONCLUSIONS:
This study was novel, as it identified clear, generalizable risk factors for LOI. In addition, our findings support the implementation of preoperative assessments to aid in care coordination and provide specific targets for intervention.
Toward an Africanized Bioethics Curriculum
Authors: BEHRENS KEVIN G. , WAREHAM C. S. 
Published: December 2020

AbstractAlthough many bioethicists have given attention to the special health issues of Africa and to the ethics of research on the continent, only a handful have considered these issues through the lens of African moral thought. The question has been for the most part neglected as to what a distinctively African moral perspective would be for the analysis and teaching of bioethics issues. To address the oversight, the authors of this paper describe embarking on a project aimed at incorporating African moral perspective, values and philosophy into a teaching curriculum. The authors clarify the rationale for the project and discuss the strategies employed in Africanizing the bioethics curriculum.
An Examination into the Embryo Disposal Practices of Human Fertilization and Embryology Authority Licenced Fertility Centers in the United Kingdom
Authors: MAGUIRE ABIGAIL 
Published: December 2020

AbstractWhen fertility centers dispose of embryos, how should this be done? Current regulatory guidelines by the Human Fertilisation and Embryology Authority state that, when terminating the development of human embryos, a clinic should act with sensitivity, taking account of the embryo’s “special status” and respecting the interests of the gamete providers and recipients. As yet, it is unclear as to how and to what extent this achieved within fertility clinics in the UK. Resultantly, this paper examines the largely undocumented domain of embryo disposal practice. By undertaking an empirical study into policy and procedure and noting divergence in clinic practice, it then comments on the ethical implications of these protocols for patients and practitioners. Specifically, this paper argues for a more holistic approach to embryo disposal. An approach that effectively meets the requirements of the lab, is respectful of the “special status” of the human embryo, and, perhaps most importantly, reflects the multifaceted needs of the patient.
Effective Communication Following Pregnancy Loss: A Study in England
Authors: AUSTIN LOUISE , LITTLEMORE JEANNETTE , MCGUINNESS SHEELAGH , TURNER SARAH , FULLER DANIELLE , KUBERSKA KAROLINA 
Published: December 2020

AbstractEach year in the UK there are approximately 250,000 miscarriages, 3,000 stillbirths and 3,000 terminations following a diagnosis of fetal-abnormality. This paper draws from original empirical research into the experience of pregnancy loss and the accompanying decisionmaking processes. A key finding is that there is considerable variation across England in the range of options that are offered for disposal of pregnancy remains and the ways in which information around disposal are communicated. This analysis seeks to outline the key features of what constitutes effective communication in this context, where effective communication is taken to mean that patients are provided with the key information necessary, in an appropriate manner, so that they are fully able to make a decision. A primary source of evidence includes interviews with the bereaved and pregnancy-loss support workers, in order to understand how the options available, and associated necessary procedures, are communicated. In addition, patient information leaflets are also analyzed as they offer an important tool for information delivery at a difficult and emotionally charged time. Following this, an overview is provided of the information that these leaflets should contain, along with guidance on effective presentation of this information.
Hourly Surface Observations Suggest Stronger Solar Dimming and Brightening at Sunrise and Sunset Over China
Authors: Wang Yawen , Zhang Jiahua , Sanchez‐Lorenzo Arturo , Tanaka Katsumasa , Trentmann Jörg , Yuan Wenping , Wild Martin 
Published: December 2020

AbstractExploration of the diurnal pattern of global dimming and brightening has been limited by the paucity of high‐temporal resolution observations. Based on 22 years’ continuous observations of hourly surface solar radiation (SSR) over 96 stations across China for 1993–2014, this study evidences higher relative changes in diurnal SSR in terms of both frequency and magnitude at sunrise and sunset, with a longer pathway for the sun passing through the atmosphere, than the hours in between. This, in general, leads to a further shortening of the days between sunrise and sunset in the cold seasons and the polluted region of north China with low radiation levels but a further extension in the other seasons and regions with sufficient sunlight. Pollution reduction by 39.4%–69.7% during the 2014 Asia‐Pacific Economic Cooperation summit at Beijing are accompanied with a 10.4–80.0% rebound of diurnal SSR in the forenoon and late afternoon.
Radiation Concerns for the Neuroanesthesiologists
Authors: Burman Sourav , Das Abanti , Mahajan Charu , Rath Girija P. 
Published: December 2020

AbstractWith the advent of minimally invasive neurosurgical techniques and rapid innovations in the field of neurointervention, there has been a sharp rise in diagnostic and therapeutic modalities requiring radiation exposure. Neuroanesthesiologists are currently involved in various procedures inside as well as outside the operating room (OR) like intensive care units, interventional suites, and gamma knife units. The ambit expands from short-lasting diagnostic scans to lengthy therapeutic procedures performed under fluoroscopic guidance. Hence, a modern-day neuroanesthesiologist has to bear the brunt of the radiation exposure in both inside and outside the OR. However, obliviousness and nonadherence to the relevant radiation safety measures are still prevalent. Radiation protection and safety are topics that need to be discussed with new vigor in the light of current practice.
Does Use of a Feeding Protocol Change Outcomes in Gastroschisis? A Report from the Midwest Pediatric Surgery Consortium
Authors: Dekonenko Charlene , Fraser Jason D. , Deans Katherine , Fallat Mary E. , Helmrath Michael , Kabre Rashmi , Leys Charles M. , Burns R Cartland , Corkum Kristine , Dillon Patrick A. , Downard Cynthia , Wright Tiffany N. , Gadepalli Samir K. , Grabowski Julia , Hernandez Edward , Hirschl Ronald , Johnson Kevin N. , Kohler Jonathan , Landman Matthew P. , Landisch Rachel M. , Lawrence Amy E. , Mak Grace Z. , Minneci Peter , Rymeski Beth , Sato Thomas T. , Slater Bethany J. , Peter St. Shawn D. 
Published: December 2020

Abstract
Introduction Gastroschisis feeding practices vary. Standardized neonatal feeding protocols have been demonstrated to improve nutritional outcomes. We report outcomes of infants with gastroschisis that were fed with and without a protocol.
Materials and Methods A retrospective study of neonates with uncomplicated gastroschisis at 11 children's hospitals from 2013 to 2016 was performed.Outcomes of infants fed via institutional-specific protocols were compared with those fed without a protocol. Subgroup analyses of protocol use with immediate versus delayed closure and with sutured versus sutureless closure were conducted.
Results Among 315 neonates, protocol-based feeding was utilized in 204 (65%) while no feeding protocol was used in 111 (35%). There were less surgical site infections (SSI) in those fed with a protocol (7 vs. 16%, p = 0.019). There were no differences in TPN duration, time to initial oral intake, time to goal feeds, ventilator use, peripherally inserted central catheter line deep venous thromboses, or length of stay. Of those fed via protocol, less SSIs occurred in those who underwent sutured closure (9 vs. 19%, p = 0.026). Further analyses based on closure timing or closure method did not demonstrate any significant differences.
Conclusion Across this multi-institutional cohort of infants with uncomplicated gastroschisis, there were more SSIs in those fed without an institutional-based feeding protocol but no differences in other outcomes.
Evaluation of a Feline Bone Surrogate and In Vitro Mechanical Comparison of Small Interlocking Nail Systems in Mediolateral Bending
Authors: Marturello Danielle M. , von Pfeil Dirsko J.F. , Déjardin Loïc M. 
Published: December 2020

Abstract
Objective The aim of this study was to (1) evaluate bending structural properties of a machined short fibre epoxy (SFE) feline bone surrogate (FBS), (2) compare the bending behaviour of small angle-stable interlocking nails (I-Loc; Targon) and locking compression plates (LCP) and (3) evaluate the effect of implant removal on FBS bending strength.
Methods Part 1: Feline cadaveric femurs (n = 10) and FBS (n = 4) underwent cyclic four-point bending and load to failure. Part 2: Fracture gap FBS constructs (n = 4/group) were stabilized in a bridging fashion with either I-Loc 3 and 4, Targon 2.5 and 3.0, LCP 2.0 and 2.4, then cyclically bent. Part 3: Intact FBS with pilot holes, simulating explantation, (n = 4/group) underwent destructive bending tests. Bending compliance, angular deformation and failure moment (FM) were statistically compared (p < 0.05).
Results Native bone and FBS were similar for all outcome measures (p > 0.05). The smallest and largest bending compliance and angular deformation were seen in the I-Loc 4 and LCP 2.0 respectively (p < 0.05). While explanted Targon FBS had the lowest FM (p < 0.05), I-Loc and LCP constructs FM were not different (p > 0.05).
Conclusion The similar bending properties of short fibre epoxy made FBS and native feline femurs suggest that this model could be used for mechanical testing of implants designed for feline long bone osteosynthesis. The I-Loc constructs smaller angular deformation which also suggests that these implants represent a valid alternative to size-matched Targon and LCP for feline fracture osteosynthesis. The significantly lower FM of explanted Targon may increase the risk of secondary fracture following implant removal.
Human Mesenchymal Stem Cell Sheets Improve Uterine Incision Repair in a Rodent Hysterotomy Model
Authors: Kuramoto Goro , Hammad Ibrahim A. , Einerson Brett D. , Allshouse Amanda A. , Debbink Michelle , Grainger David W. , Silver Robert M. , Okano Teruo 
Published: December 2020

Objective The study aimed to assess the feasibility of creating and transplanting human umbilical cord mesenchymal stem cell sheets applied to a rat model of hysterotomy, and additionally to determine benefits of human umbilical cord mesenchymal stem cell sheet transplantation in reducing uterine fibrosis and scarring.
Study Design Human umbilical cord mesenchymal stem cell sheets are generated by culturing human umbilical cord mesenchymal stem cells on thermo-responsive cell culture plates. The temperature-sensitive property of these culture dishes facilitates normal cell culture in a thin contiguous layer and allows for reliable recovery of intact stem cell sheets without use of destructive proteolytic enzymes.We developed a rat hysterotomy model using nude rats. The rat uterus has two distinct horns: one horn provided a control/untreated scarring site, while the second horn was the cell sheet transplantation site.On day 14 following surgery, complete uteri were harvested and subjected to histologic evaluations of all hysterotomy sites.
Results The stem cell sheet culture process yielded human umbilical cord mesenchymal stem cell sheets with surface area of approximately 1 cm2.Mean myometrial thickness in the cell sheet-transplanted group was 274 μm compared with 191 μm in the control group (p = 0.02). Mean fibrotic surface area in the human umbilical cord mesenchymal stem cell sheet-transplanted group was 95,861 μm2 compared with 129,185 μm2 in the control group. Compared with control horn sites, cell sheet-transplanted horns exhibited significantly smaller fibrotic-to-normal myometrium ratios (0.18 vs. 0.27, respectively, p = 0.029). Mean number of fibroblasts in cell sheet-transplanted horns was significantly smaller than the control horns (483 vs. 716/mm2, respectively, p = 0.001).
Conclusion Human umbilical cord mesenchymal stem cell sheet transplantation is feasible in a rat model of hysterotomy. Furthermore, use of stem cell sheets reduces fibroblast infiltration and uterine scar fibrotic tissue formation during hysterotomy healing, potentially mitigating risks of uterine scar formation.
Key Points
A Multicenter Evaluation of Paradoxical Adipose Hyperplasia Following Cryolipolysis for Fat Reduction and Body Contouring: A Review of 8658 Cycles in 2114 Patients
Authors: Nikolis Andreas , Enright Kaitlyn M 
Published: December 2020

Abstract

Background
Paradoxical adipose hyperplasia (PAH) is a rare, moderate-to-severe adverse event associated with cryolipolysis (CoolSculpting, CS).

Objectives
The aim of this study was to describe the incidence, diagnosis, and treatment of PAH occurring after CS for nonsurgical fat reduction.

Methods
A multicenter evaluation of all patients who underwent CS treatment between January 2015 and December 2019 at 8 Canadian medical centers was conducted. Data abstracted included symptoms, management strategy, outcome, operator characteristics, device characteristics, patient characteristics, body region, and CS treatment details. Incidence of PAH was calculated based on the number of treatment cycles.

Results
Our findings revealed incidence rates between 0.05% and 0.39%, which are slightly higher than the manufacturer’s quoted rate of 0.025% (1 per 4000 cycles). Incidence rates at all sites were dramatically reduced by over 75% with the implementation of newer models of CS units. Of patients who developed PAH, 55% were male and 77.8% were of European ethnic origin. The majority of cases (76.9%) were associated with older models of CS units.

Conclusions
Development of PAH may be related to a combination of factors, including older models of CS units and applicators, as well as individual characteristics that predispose certain patients.

Level of Evidence: 4
FifBase: a comprehensive fertility-associated indicators factor database for domestic animals
Authors: Li Hao , Hou Junyao , Chen Ziyu , Zeng Jingyu , Ni Yu , Li Yayu , Xiao Xia , Zhou Yaqi , Zhang Ning , Long Deyu , Liu Hongfei , Yang Luyu , Bai Xinyue , Li Qun , Li Tongtong , Che Dongxue , Li Leijie , Wang Xiaodan , Zhang Peng , Liao Mingzhi 
Published: December 2020

Abstract
Fertility refers to the ability of animals to maintain reproductive function and give birth to offspring, which is an important indicator to measure the productivity of animals. Fertility is affected by many factors, among which environmental factors may also play key roles. During the past years, substantial research studies have been conducted to detect the factors related to fecundity, including genetic factors and environmental factors. However, the identified genes associated with fertility from countless previous studies are randomly dispersed in the literature, whereas some other novel fertility-related genes are needed to detect from omics-based datasets. Here, we constructed a fertility index factor database FifBase based on manually curated published literature and RNA-Seq datasets. During the construction of the literature group, we obtained 3301 articles related to fecundity for 13 species from PubMed, involving 2823 genes, which are related to 75 fecundity indicators or 47 environmental factors. Eventually, 1558 genes associated with fertility were filtered in 10 species, of which 1088 and 470 were from RNA-Seq datasets and text mining data, respectively, involving 2910 fertility-gene pairs and 58 fertility-environmental factors. All these data were cataloged into FifBase (http://www.nwsuaflmz.com/FifBase/), where the fertility-related factor information, including gene annotation and environmental factors, can be browsed, retrieved and downloaded with the user-friendly interface.