bluuebunny/crossref_metadata_2025_split huggingface dataset:
Congenital right diaphragmatic hernia in an adult
Authors: Al-Zayer Faisal , Aljaroof Abdulla H , Al-Marhoun Maram , Abualsaud Basem , Al-Zaher Mohammed , Meshikhes Abdul-Wahed 
Published: November 2019

AbstractDiaphragmatic hernia in the absence of trauma in adults is very rare. It occurs as a result of unilateral diaphragmatic agenesis. The diagnosis of this rare condition is typically made in early infancy. However, in asymptomatic patients, the diagnosis is often delayed for months and even years. We present a case of a 27-year-old female, who was referred 48-hours after Caesarean section with suspected pulmonary embolism. Computed tomography scan revealed herniation of the liver as well as bowel loops into the right hemi-thorax. Exploration through a right thoracotomy revealed right diaphragmatic agenesis. The contents were reduced into the abdomen, and the defect was repaired using a mesh. The patient had an uneventful postoperative recovery and was discharged home 10 days later. This case highlights the acute late presentation of right diaphragmatic eventration with abdominal visceral herniation in adulthood. The condition may be triggered by the increasing size of gravid uterus.
‘As though Miles of Ocean did not Separate us’: Print and the Construction of a Transatlantic Free Love Community at the Fin de Siècle
Authors: Jones Sarah L 
Published: November 2019

Abstract
This article argues that British and American free lovers – radical sexual reformers committed to the cause of ‘sexual freedom’ – came together through print to build a transatlantic community at the fin de siècle. Challenging existing narratives that characterize free love as isolated or incoherent, it argues that through print free lovers from Britain and America were able to forge links with each other, and to construct an important, coherent collective identity that transcended national boundaries. In doing so it makes two major interventions. First, it provides unique new insights into the history of free love in both the British and American contexts, placing a new focus on often overlooked transnational connections and exchanges that helped to shape late nineteenth-century free love campaigns. Second, it encourages historians to rethink the ways we look for and make sense of cohesive international reform communities more broadly in this period. By exploring how a small, radical group like the free lovers were able to cohere through processes of contestation and negotiation played out entirely in print, this article will show that, where necessary, print was enough for transatlantic reformers to construct common identities and negotiate coherent reform ideas. As such, it argues that historians of fin-de-siècle social reform should look again at the print culture of other contemporary reformers otherwise labelled divided, isolated, or marginalized to look for threads of cohesion, cooperation, and compromise.
Negative Alcohol and Tobacco Consumption Behaviors in an Australian Army Combat Brigade
Authors: Selman Jason , Rogers Rona , Molloy Sara 
Published: November 2019

Abstract

Introduction
Western militaries have consumption rates of alcohol and tobacco which are higher than the associated civilian populations, and are concerned about the negative effects on health, wellbeing, and military performance that such high rates may have.

Materials and Methods
A survey questionnaire which collected nonidentifiable data was distributed to all accessible members of an Australian Army Combat Brigade over the annual induction training and prefield exercise period between January and March 2019. The survey was composed of personal and demographic questions, followed by questions concerning the personal consumption habits of alcohol and tobacco. 1,606 complete and valid surveys were returned, representing 55.3% of the Brigade’s posted strength. Ethical clearance for this project was provided by the Australian Defence Force Joint Health Command Low Risk Ethics Panel (18-012).

Results
Almost one quarter of the Combat Brigade exceeded the Australian daily risk guideline for alcohol consumption, and over three quarters exceeded the occasion risk guideline; with 6.1% of soldiers drinking alcohol daily. 24.3% identified as tobacco smokers, with 15.9% smoking daily. Smoking rates and volumes were higher when deployed on field training exercises and on international training and operational deployments; as was the consumption of roll-your-own cigarettes. Overall, the main determinants of the negative consumption behaviors were gender (male) and age (under 25).

Conclusions
This study confirmed that the members of an Australian Army Combat Brigade have higher negative health consumption behaviors concerning alcohol and tobacco than the general civilian population. The results also identified a cohort of Senior Non-Commissioned Officers and Warrant Officers (E5–E9) between the ages of 35 and 44 who had high negative consumption behaviors which could result in poor health outcomes. This could be a suitable cohort for a targeted campaign to reduce tobacco and alcohol consumption and to assist to make healthy life changes.
Neonatal follow-up programs in Canada: A national survey
Authors: Albaghli Fawaz , Church Paige , Ballantyne Marilyn , Girardi Alberta , Synnes Anne 
Published: November 2019

Abstract

Background
A 2006 Canadian survey showed a large variability in neonatal follow-up practices. In 2010, all 26 tertiary level Neonatal Follow-Up clinics joined the Canadian Neonatal Follow-Up Network (CNFUN) and agreed to implement a standardized assessment (including the Bayley Scales of Infant and Toddler Development-III (Bayley-III) at 18 months corrected age for children born &lt; 29 weeks’ gestation. It is unknown whether the variability in follow-up practices lessened as a result.

Objectives
To describe the current status of neonatal follow-up services in Canada and changes over time.

Methods
A comprehensive online survey was sent to all tertiary level CNFUN Follow-up programs. Questions were based on previous survey results, current literature, and investigator expertise and consensus.

Results
Respondents included 23 of 26 (88%) CNFUN programs. All sites provide neurodevelopmental screening and referrals in a multidisciplinary setting with variations in staffing. CNFUN programs vary with most offering five to seven visits. Since 2006, assessments at 18 months CA increased from 84% to 91% of sites, Bayley-III use increased from 21% to 74% (P=0.001) and eligibility for follow-up was expanded for children with stroke, congenital diaphragmatic hernia and select anomalies detected in utero. Audit data is collected by &gt; 80% of tertiary programs.

Conclusion
Care became more consistent after CNFUN; 18-month assessments and Bayley-III use increased significantly. However, marked variability in follow-up practices persists.
Translational Landscape of Protein-Coding and Non-Protein-Coding RNAs upon Light Exposure in Arabidopsis
Authors: Kurihara Yukio , Makita Yuko , Shimohira Haruka , Fujita Tomoya , Iwasaki Shintaro , Matsui Minami 
Published: November 2019

Abstract
Light is one of the most essential environmental clues for plant growth and morphogenesis. Exposure to blue monochromatic light from darkness is a turning point for plant biological activity, and as a result dramatic changes in gene expression occur. To understand the translational impacts of blue light, we have performed ribosome profiling analysis and called translated open reading frames (ORFs) de novo within not only mRNAs but also non-coding RNAs (ncRNAs). Translation efficiency of 3,823 protein-coding ORFs, such as nuclear chloroplast-related genes, was up-regulated by blue light exposure. Moreover, the translational activation of the microRNA biogenesis-related genes, DCL1 and HYL1, was induced by blue light. Considering the 3-nucleotide codon periodicity of ribosome footprints, a few hundred short ORFs lying on ncRNAs and upstream ORFs (uORFs) on mRNAs were found that had differential translation status between blue light and dark. uORFs are known to have a negative effect on the expression of the main ORFs (mORFs) on the same mRNAs. Our analysis suggests that the translation of uORFs is likely to be more stimulated than that of the corresponding mORFs, and uORF-mediated translational repression of the mORFs in five genes was alleviated by blue light exposure. With data-based annotation of the ORFs, our analysis provides insights into the translatome in response to environmental changes, such as those involving light.
Education Needs of Dutch Physical Therapists for the Treatment of Patients With Advanced Cancer: A Mixed Methods Study
Authors: Ten Tusscher Marieke R , Groen Wim G , Geleijn Edwin , Berkelaar Dagmar , Aaronson Neil K , Stuiver Martijn M 
Published: November 2019

Abstract

Background
The survival rates for patients with advanced cancer have increased over time. Many patients experience symptoms and functional limitations that impair activities of daily living and limit quality of life. A number of these health problems are amenable to physical therapist treatment. However, physical therapists caring for patients with advanced cancer require special training and skills.

Objective
The study aimed to assess the educational needs and clinical uncertainties of Dutch physical therapists in relation to treatment of patients with advanced cancer.

Design
This was a mixed methods study.

Methods
A survey and 2 focus groups were conducted among physical therapists working in primary care who had previously received at least basic oncology training.

Results
A total of 162 physical therapists completed the survey. The most frequently reported educational needs were related to effective interprofessional collaboration (61.7%), knowledge of medical treatment (49.4%), and current evidence on physical therapist interventions in this population (49.4%). In the focus groups, physical therapists (n = 17) voiced uncertainties about treating patients with bone metastases, setting realistic goals, when and how to end a treatment episode, interprofessional collaboration, finding and using evidence, and using clinimetrics.

Conclusion
These results support the need for specific education programs for physical therapists working with advanced cancer patients to increase the availability of high-quality oncology rehabilitation for this population.
The Practice-Based Implementation (PBI) Network: Technology (Tech) into Care pilot
Authors: Pratt Kimberly M , Branch LaToya Z , Houston Jorielle B 
Published: November 2019

Abstract
Technology can support the use of evidence-based behavioral health treatments, as well as serve as a link to care to improve patient engagement and adherence to treatment. While research suggests that mobile applications (apps) specifically have the potential to enhance the delivery of behavioral health treatment, little guidance exists on how clinicians can effectively and safely integrate mobile apps into their clinical practice. The purpose of this paper is to describe the Department of Defense Practice-Based Implementation (PBI) Network’s approach to implementation and present the results of the Technology (Tech) into Care pilot. The PBI Network conducted this pilot to address providers’ knowledge gaps regarding the integration of mobile apps into behavioral health care within the Military Health System (MHS) and examine the feasibility of widespread dissemination of the practice change in the MHS.
A mixed-method design guided by the Promoting Action on Research Implementation in Health Services framework was used to evaluate the approach and identify facilitators and barriers to practice change.
Positive preliminary support for the feasibility of the Tech into Care approach was demonstrated in improvements in providers’ knowledge, comfort, and perceived level of preparation to integrate mobile apps into care. Organizational challenges including time constraints and staff turnover negatively impacted implementation success. The PBI Network Tech into Care implementation approach is a feasible method to address knowledge gaps related to the integration of mobile apps into clinical practice within the MHS. Contextual factors, such as competing time demands, often impede providers’ ability to effectively integrate mobile apps into care.
Loss of BICD2 in muscle drives motor neuron loss in a developmental form of spinal muscular atrophy
Authors: Rossor AM , Sleigh JN , Groves M , Muntoni F , Reilly MM , Hoogenraad CC , Schiavo G 
Published: November 2019

AbstractBICD2 is a key component of the dynein/dynactin motor complex. Autosomal dominant mutations in BICD2 cause Spinal Muscular Atrophy Lower Extremity Predominant 2 (SMALED2), a developmental disease of motor neurons. In this study we sought to examine the motor neuron phenotype of conditional Bicd2−/− mice. Bicd2−/− mice show a significant reduction in the number of motor axons of the L4 ventral root compared to wild type mice. Muscle-specific knockout of Bicd2, but not motor neuron-specific Bicd2 loss, results in a reduction in L4 ventral axons comparable to global Bicd2−/− mice. Rab6, a small GTPase required for the sorting of secretory vesicles from the TGN to the plasma membrane is a major binding partner of BICD2. We therefore examined the secretory pathway in SMALED2 patient fibroblasts and demonstrated impaired flow of constitutive secretory cargoes. Together, these data indicate that BICD2 loss from muscles is a major driver of non-cell autonomous pathology with important implications for future therapeutic approaches to SMALED2.SummaryMissense mutations in the cargo adaptor protein BICD2 cause SMALED2, a developmental disease of motor neurons. In this study, the authors show that BICD2 mutations cause motor neuron loss by a non-cell autonomous mechanism determining a disabling impairment of muscle function.
An optimized 16S rRNA sequencing protocol for vaginal microbiome to avoid biased abundance estimation
Authors: Zhang Qiongqiong , Zhang Lei , Wang Ying , Zhao Meng , Chen Rui , Tao Zhi , Lyu Tao , Huang Zhenyu , Liao Qinping 
Published: November 2019

AbstractWe applied three 16S rRNA sequencing protocols on vaginal microbiome samples, to evaluate whether they produce unbiased estimation of vaginal microbiome composition. We modified the 27F primer (hereafter denoted as 27F’). Using vaginal samples from 28 healthy women and 10 women with bacterial vaginosis, we sequenced three 16S rRNA sequencing protocols, i.e., 27F-338R, 27F’-338R and 341F-806R protocols, naming after their PCR primer sets, to test whether the sequencing results are consistent with the clinical diagnostics, morphology and qPCR results. First, the 27F primer would not align withGardnerlla vaginalisvery well, leading to poor amplification of such species. By modifying the primer sequences, the modified 27F primer (27F’) was able to amplifyGardnerlla vaginalisvery well. Second, the DNA sequence of characteristic speciesLactobacillus crispatusis identical withLactobacillus garrinarum, leading to biased estimation of abundance ofLactobacillus crispatuswhen using V3-V4 as PCR target region; in contrast, such bias did not occur when using V1-V2 as a target region. Third, optimized 27F’-338R avoided above-mentioned biases and restored the well-established community state types (CSTs) clustering.ImportanceVaginal microbiome has profound effects on the health of women and their newborns. Our study found that two well-established 16S rDNA sequencing protocols led to systementical biased estimation of characteristic species of vaginal microbiome. Subsequent analysis proved that the PCR primer fetching efficacy and target region identity were major contributor for such bias. With carefully selected target region and optimized PCR primer set, we were able to eliminate such biases and provide accurate estimation of vaginal microbiome, which showed high consistency with clinical diagnostics. We modified the 27F primer (27F’). Using the optimized PCR primer set of 27F’ and 338R to target the V1-V2 hyper-variable region, our 16S rRNA sequencing correctly evaluate the composition of vaginal microbiome.
An improved growth medium for enhanced inoculum production of the plant growth-promoting fungusSerendipita indica
Authors: Osman Mohamed , Stigloher Christian , Mueller Martin J. , Waller Frank 
Published: November 2019

AbstractThe plant endophytic fungusSerendipita indicacolonizes roots of a wide range of plant species and can enhance growth and stress resistance of these plants. Due to its ease of axenic cultivation and its broad host plant range including the model plant Arabidopsis thaliana and numerous crop plants, it is widely used as a model fungus to study beneficial fungus-root interactions. In addition, it was suggested to be utilized for commercial applications, e.g. to enhance yield in barley and other species. To produce inoculum,S. indicais mostly cultivated in a complex Hill-Käfer medium (CM medium), however, growth in this medium is slow, and yield of chlamydospores, which are often used for plant root inoculation, is relatively low. We tested and optimized a simple vegetable juice-based medium for an enhanced yield of fungal inoculum. The described vegetable juice (VJ) medium is based on commercially available vegetable juice and is easy to prepare. VJ medium was superior to the currently used CM medium with respect to biomass production in liquid medium and hyphal growth on agar plates. Using solid VJ medium supplemented with sucrose (VJS), a high amount of chlamydospores developed already after 8 days of cultivation, producing significantly more spores than on CM medium. Use of VJ medium is not restricted toS. indica, as it also supported growth of two pathogenic fungi often used in plant pathology experiments: the ascomyceteFusarium graminearum, the causal agent of Fusarium head blight disease on wheat and barley, andVerticillium longisporum, the causal agent of verticillium wilt. The described VJ medium is recommended for a streamlined and efficient production of inoculum for the plant endophytic fungusSerendipita indicaand might prove superior for the propagation of other fungi for research purposes.
Adventures in Multi-Omics I: Combining heterogeneous datasets via relationships matrices
Authors: Akdemir Deniz , Knox Ron , Isidro-Sánchez Julio 
Published: November 2019

AbstractPrivate and public breeding programs, as well as companies and universities, have developed different genomics technologies which have resulted in the generation of unprecedented amounts of sequence data, which bring new challenges in terms of data management, query, and analysis. The magnitude and complexity of these datasets bring new challenges but also an opportunity to use the data available as a whole. Detailed phenotype data, combined with increasing amounts of genomic data, have an enormous potential to accelerate the identification of key traits to improve our understanding of quantitative genetics. Data harmonization enables cross-national and international comparative research, facilitating the extraction of new scientific knowledge. In this paper, we address the complex issue of combining high dimensional and unbalanced omics data. More specifically, we propose a covariance-based method for combining partial datasets in the genotype to phenotype spectrum. This method can be used to combine partially overlapping relationship/covariance matrices. Here, we show with applications that our approach might be advantageous to feature imputation based approaches; we demonstrate how this method can be used in genomic prediction using heterogenous marker data and also how to combine the data from multiple phenotypic experiments to make inferences about previously unobserved trait relationships. Our results demonstrate that it is possible to harmonize datasets to improve available information across gene-banks, data repositories or other data resources.Key messageSeveral covariance matrices obtained from independent experiments can be combined as long as these matrices are partially overlapping. We demonstrate the usefulness of this methodology with applications in combining data from several partially linked genotypic and phenotypic experiments.Author contribution statement–DA: Conception or design of the work, statistics, R programs, simulations, drafting the article, and critical revision of the article.–JIS: R programs, graphs, drafting the article, critical revision of the article.–RK: Critical revision of the article.
Transgenic mice expressing human alpha-synuclein in noradrenergic neurons develop locus coeruleus pathology and non-motor features of Parkinson’s disease
Authors: Butkovich LM , Houser MC , Chalermpalanupap T , Porter-Stransky KA , Iannitelli AF , Boles JS , Lloyd GM , Coomes AS , Eidson LN , De Sousa Rodrigues ME , Oliver DL , Kelly SD , Chang J , Bengoa-Vergniory N , Wade-Martins R , Giasson BI , Joers V , Weinshenker D , Tansey MG 
Published: November 2019

AbstractDegeneration of locus coeruleus (LC) neurons and dysregulation of noradrenergic signaling are ubiquitous features of Parkinson’s disease (PD). The LC is among the first brain regions affected by α-synuclein (asyn) pathology, yet how asyn affects these neurons remains unclear. LC-derived norepinephrine (NE) can stimulate neuroprotective mechanisms and modulate immune cells, while dysregulation of NE neurotransmission may exacerbate disease progression, particularly non-motor symptoms, and contribute to the chronic neuroinflammation associated with PD pathology. Although transgenic mice overexpressing asyn have previously been developed, transgene expression is usually driven by pan-neuronal promoters and thus has not been selectively targeted to LC neurons. Here we report a novel transgenic mouse expressing human wild-type asyn under control of the noradrenergic-specific dopamine β-hydroxylase promoter. These mice developed oligomeric and conformation-specific asyn in LC neurons, alterations in hippocampal and LC microglial abundance, upregulated GFAP expression, degeneration of LC fibers, decreased striatal dopamine (DA) metabolism, and age-dependent behaviors reminiscent of non-motor symptoms of PD that were rescued by adrenergic receptor antagonists. These mice provide novel insights into how asyn pathology affects LC neurons and how central noradrenergic dysfunction may contribute to early PD pathophysiology.Significance statementα-synuclein (asyn) pathology and loss of neurons in the locus coeruleus (LC) are two of the most ubiquitous neuropathologic features of Parkinson’s disease (PD). Dysregulated NE neurotransmission is associated with the non-motor symptoms of PD including sleep disturbances, emotional changes such as anxiety and depression, and cognitive decline. Importantly, loss of central NE may contribute to the chronic inflammation in, and progression of, PD. We have generated a novel transgenic mouse expressing human asyn in LC neurons to investigate how increased asyn expression affects the function of the central noradrenergic transmission and associated behaviors. We report cytotoxic effects of oligomeric and conformation-specific asyn, astrogliosis, LC fiber degeneration, disruptions in striatal dopamine metabolism, and age-dependent alterations in non-motor behaviors without inclusions.