bluuebunny/crossref_metadata_2025_split huggingface dataset:
Efficiency of the use of mutations, induced on radiation-contaminated areas, when improving winter wheat cultivars
Authors: Yakymchuk R. A. 
Published: March 2020

Aim. Breeding of Triticum aestivum L. cultivars requires the development of generically diversified primary material. Induced mutagenesis plays an important role in the solution of the issue. Mutagenic activity of radionuclide contaminations of the alienation zone of ChNPP and industrial facilities of uranium manufacture was studied to determine the efficiency of their use when developing the material of winter wheat which is valuable for breeding. Methods. The selection of mutants was done using the methods of recording and singling out of visible mutations, phenological observations, analysis of biometric indicators, determination of total yield capacity and its components. Results. Chronic effect of the radiation of radionuclide contaminations of the alienation zone of ChNPP and the territory of uranium ore mining results in the increase of mutation variability of winter wheat by 8–14.9 and 9.8–12.6, respectively. Mutation spectrum included 24.29–49.34 % of those valuable for breeding. Productive mutants, which exceeded primary cultivars in crop capacity by 2.7–6.9 %, were developed. Mutant samples, whose high yield capacity was supplemented with grain quality enhancement or its maintenance at the level of a primary form, were identified. Conclusions. The expansion of genetic variety of primary breeding material due to mutagenesis, induced by radionuclide contaminations, creates the opportunities for its use in hybridization aimed at the implementation of breeding-genetic programs of the improvement of wheat cultivars.
Keywords: Triticum aestivum L., radionuclide contamination, mutation variability, valuable for breeding mutations, productive forms.
The effect of various NaCl concentrations on morphophysiological properties of bread wheat sprouts (Triticum aestivum L.)
Authors: Gasimova F. I. , Khanishova M. A. , Taghiyeva K. R. , Azizov I. V. 
Published: March 2020

Aim. The effects of various NaCl concentrations on germination energy, germination percentage of seeds and morpho-physiological indices of seedlings have been determined in bread wheat (Triticum aestivum L.) varieties. Methods. Wheat seeds were germinated at 0, 100, 150, 200 mM concentrations of NaCl using the roll method. Rezults. The delay of the growth of seedlings and root system was observed with increasing salt concentrations in all samples. The effect of various salt concentrations on separate varieties was diifferent. Conclusions. The highest indices of the development of coleoptiles and root system and the highest amount of chlorophyll were detected at 150 and 200 mM concentrations of NaCl in the Mirbashir-128 variety. The Bezostaya and Nurlu-99 varieties manifested medium tolerance, while the Sheki-1 variety was relatively less tolerant.
Keywords: NaCl, wheat varieties, germination energy, germination percentage, chlorophyll.
Renewing DNA integrity by cell repair systems
Authors: Lukash L. L. 
Published: March 2020

The four of six main cell repair systems have been reviewed. Some of them have been studied by Nobel laureates of 2015 year in the field of chemistry. The common title of their work is “For investigation of DNA repair mechanisms”. Numerous primer damages of DNA induced by exogenous and endogenous factors are corrected by complex cell systems including repair enzymes and other proteins. Mutational changes in the genes coding the repair enzymes are the most dangerous because they dramatically increase mutation frequency in pro- and eukaryotic cells. But due to the constant and correct action of cell repair enzymes DNA integrity is renewing, that is why the organisms on our planet are still alive and functionally active.
Keywords: DNA repair, reparative enzymes, pro- and eukaryotic cells, primer damages, mutations.
Comparative cytogenetic analysis of early reproductive losses depending of maternal age
Authors: Tkach I. R. , Huleyuk N. L. , Zastavna D. V. , Sosnina K. O. , Bezkorovaina G. M. , Helner N. V. 
Published: March 2020

Aim. Human reproduction characterized by a high incidence of aneuploidies. Approximately 99 % of conceptions with anomalies of karyotype terminate of pregnancy loss, mainly during the first 14 weeks of intrauterine development. The frequency and spectrum of karyotype anomalies in the chorionic villus of early reproductive losses were studied depending of maternal age. Methods. Banding cytogenetic and interphase mFISH with the centromeric probe panel for chromosomes 13, 14, 15, 16, 17, 18, 21, 22, X and Y were used. Results. The contribution of different karyotype abnormalities in the genesis of the early reproductive losses depends on the age of the woman, namely, with age significantly increases the proportion of aneuploidy due to autosomal trisomies and reduced contribution of polyploidy and gonosomal monosomy. The main autosomal trisomy in the material of lost pregnancies from women under the age of 35 is 16, 21 and 15, 22, 13 and 18 in order of decreasing frequency, and from older women 16, 15, 22, 21, 13 and 14. Conclusions. The structure and rate of karyotype anomalies in the material of lost pregnancies varies with maternal age.
Keywords: early reproductive loss, maternal age, karyotype abnormalities.
The frequency of genetic factors of thrombophilia in women with recurrent pregnancy losses
Authors: Chorna L. B. , Makukh H. V. , Zastavna D. V. , Prokopchuk N. V. , Helner N. V. 
Published: March 2020

Aim. There is growing evidence that recurrent pregnancy losses (RPL) are associated with the presence of inherited thrombophilias but data are inconsistent. The present study aimed to assess the distribution of inherited risk factors of thrombophilia among women with RPL. Methods. We studied 68 women with RPL and 120 healthy women of control group, inhabitants of Western Ukraine. In all subjects the detection of genetic factors of thrombophilia were determined by polymerase chain reaction and restriction fragment length polymorphism method. Results. The prevalence of heterozygotes for FV 1691G/A among women with RPL and controls were: 12 % versus 4 %, respectively. Women heterozygosity for factor V Leiden was significantly more prevalent in the RPL group than in controls (OR 3.11, 95 % CI 1.02–9.46). Results showed that carriers of PAI-1 4G allele have increased odds on more than 2 times in comparison to the carriers of homozygous 5G5G genotypes. A significant relationship between allele variation 677T of MTHFR gene (OR 1.70, 95 % CI 1.09–2.67) and RPL was observed. Conclusions. Significance of 1691G/A mutation of V blood coagulation factor gene, alleles variations of 677T of MTHFR gene and 4G of PA1-1 gene in the structure of predisposition to RPL in group of west Ukrainian women was established.
Keywords: genetic factors, hereditary thrombophilia, molecular genetic testing, RPL.
Effects of genes controlling the plant development rates on the formation of individual productivity of wheat and soybean
Authors: Avksentiieva O. A. , Zubrich A. I. , Vasilchenko M. S. , Shulik V. V. 
Published: March 2020

Aim. The aim of the work was to study the effects of genes controlling the plant development rates on the speed of transition to generative development and formation of individual productivity of isogenic lines of wheat (Triticum aestivum L.) and soybean plants (Glycine max (L.) Merr.) according to the genes. Methods. The experiments were carried out in the field conditions. The periods of sprouting and earing (PSE) of wheat, the periods of sprouting and flowering (PSF) and ripening (PSR) of soybean isogenic lines and the elements of the structure of individual productivity were determined. Results. It was shown that under conditions of a natural long day (16 hours), wheat plants with genotypes VRN-A1a, VRN-D1a, PPD-A1a and PPD-D1a more rapidly passed to the earing. In soybean isogenic lines, the presence of dominant alleles of the E-series genes increased the duration of phases of PSF and PSR. The maximum indicators of productivity elements were characterized by isogenic lines, developed at a faster rate. Conclusions. The genes controlling the development rates of soft wheat and soybean crops, defining the timing of the transition to generative development, determine their productivity.
Keywords: Triticum aestivum L., Glycine max (L.) Merr., VRN, PPD, EE genes, rates of development, productivity.
The resistance of chernozem soil microorganisms to soluble copper compounds
Authors: Havryliuk O. A. , Govorukha V. M. , Tashyrev A. B. 
Published: March 2020

Аim. Determination of resistance of Ukrainian chernozem soil microorganisms to the influence of toxic copper(II). Methods. Content of copper-resistant microorganisms in chernozem was determined by counting colonies on a solid nutrient medium containing Cu(II). Resistance of microorganisms was determined by cultivation in the medium with concentration gradient of Сu2+. Results. Microorganisms resistant to toxic copper(II) by ultrahigh concentrations were shown to be present in chernozem soil. Microorganisms grew in the medium containing up to 500 mg/l Cu2+ (CuSO4 solution) and up to 10000 mg/l Cu2+ (in complex with citrate). Chelation of copper(II) with citrate was found to lead to increase of microbial resistance in 20 times. It was determined that a vanishingly small number of microorganisms resistant to toxic copper by ultrahigh concentrations can exist in a natural ecosystem. The number of viable microorganisms decreases with the increase in the content of Cu2+ that is described by the hyperbolic curve. Conclusions. The proposed methodological approach not only allows to isolate copper-resistant microorganisms from natural ecosystems of all geographic zones of the globe, but also avoid complex genetic transformations in order to obtain perspective genetically modified strains for further application in biotechnologies for purification of industrial wastewater.
Keywords: copperresistant microorganisms, chernozem soil of Ukraine, diversified microbial community, environmental biotechnologies.
Development and studying of Triticum aestivum L. material with introgressions from Aegilops biuncialis Vis.
Authors: Kozub N. A. , Sozinov I. A. , Bidnyk H. Ya. , Demianova N. A. , Sozinova A. I. , Karelov A. V. , Pylypenko L. A. , Blume Ya. B. , Sozinov A. A. 
Published: March 2020

Aim. The aim of the study was to develop and study T. aestivum material with introgressions from Ae. biuncialis. Methods. Quantitative traits of F4 lines from crossing wheat with Crimean accessions of Ae. biuncialis were studied. SDS and APAG electrophoreses of storage proteins were used to identify alleles at the Glu-1 and Gli-1 loci, including introgressed ones. Results. F4 lines from crosses of wheat with Ae. biuncialis showed a wide range of yield traits. Some lines had a clavate spike and a hairy leaf blade. Using storage proteins as genetic markers the presence of chromosome 1U was identified among the progeny of plants analyzed; some of them had translocation of arm 1UL. Lines with introgressions of chromosome 1M were not revealed. Conclusions. T. aestivum lines with introgressions from Ae. biuncialis were developed without amphidiploid production. Lines with chromosome 1U were selected, including lines with translocations of arm 1UL.
Keywords: Triticum aestivum L., Aegilops biuncialis Vis., introgression.
Influence of various factors on the antiradical activity of Panax ginseng С. A. Meyer extracts
Authors: Reshetnikov V. N. , Shutava H. G. , Shysh S. N. , Gil T. V. 
Published: March 2020

Aim. The aim of the work was to evaluate the anti-radical activity of extracts of ginseng's underground and overground organs Panax ginseng C.A. Mey, depending on various factors – age of plants, collection time and cultivation conditions. Methods. Extracts obtained from various plant organs were evaluated in the model system with cation-radicals ABTS. At the same time, the amount of extractive substances and phenolic compounds was determined. Results. Samples of leaves and roots of ginseng of 1, 2, 3, 5, 9 years of vegetation from the collection of the Central Botanical Garden of the National Academy of Sciences of Belarus were analyzed. It is established that extracts of ginseng leaves of P. ginseng can be considered as sufficiently effective anti-radical agents. Anti-radical activity of extracts of roots and leaves of ginseng differed significantly depending on the weather conditions of the growing season. Conclusions. It is shown that extracts of ginseng leaves differ by the maximum antiradical activity. Extracts of ginseng roots showed a much lower anti-radical activity in comparison with leaf extracts, which may be due to both a smaller amount of extractives extracted from water-ethanol extraction and their composition.
Keywords: ginseng, antiradical activity, phenolic compounds, extractives.
Development of caryological and molecular-genetic investigations of wetland and dryland populations of conifers
Authors: Sedel'nikova T. S. 
Published: March 2020

Aim. On the basis of studies which carried out by various authors, regularities of karyotypic and genetic divergence of populations of conifers growing in wetlands and dry lands are analyzed. Methods. The karyological and molecular-genetic methods of analysis are used. Results. Data are obtained on possible ways of evolution of karyotypes, consisting in changes in the number of chromosomes, the size and morphology of chromosomes, the number of secondary constrictions in chromosomes, the spectrum of chromosome rearrangements, and the frequency of their occurrence, distinguishing wetland populations of conifers species from dry land ones. With the help of isozyme markers and markers of nuclear and cytoplasmic genomes differences in the genetic structure of conifers populations from wetlands and dry lands are revealed. Conclusions. The genotypic structure of conifers species in wetlands and dry lands, caused by different vector of natural selection in contrasting ecological conditions of growth, differs significantly, and their karyotypic and genetic differentiation corresponds to the level of populations and groups of populations.
Keywords: populations of conifers, wetlands, dry lands, karyological and molecular-genetic markers.
Rosana – a new variety of alfalfa (Medicago sativa L.)
Authors: Bugayov V. D. , Gorensky V. M. , Mamaliga V. S. , Maximov A. M. 
Published: March 2020

Aim. Lucerne sowing in Ukraine is one of the most common fodder crops from perennial legumes. Most varieties grown today are not sufficiently adapted to a number of unfavorable abiotic and biotic environmental factors. Rosana variety was created to solve some of the above problems under the conditions of the Polissya and the forest-steppe of Ukraine. Methods. 560 plants, differing in high winter resistance and a set of economic and valuable features, were selected from the grassland of Regent's varieties of alfalfa, created in previous years from genetic plasma of varieties WL 202 (USA) and Veselopodolianska 11 (Ukraine). During the four study cycles, gumboil counts were carried out at the level of green mass and seed yield, leafyness, resistance to pathogens of root rot and other diseases, intensity of regrowth in spring and autumn. Results. The Rosana variety is similar to the Regina variety in the form of a bush, the size of leaves, the height of plants, the duration of individual phases of growth and development, and the class of rest. The variety is characterized by high intensity of regrowth, during the growing season can form 3–4 slopes, with increased feed quality, increased seed yield. Resistant to root rot, drought-proof and winter-resistant. Resistant to sinking. Medieval The period of productive longevity is 4–5 years. The height of the plants of the first slope is 80–90 cm. The yield of the seeds is 0.5 t / ha. Collection of dry matter – 14.5–15.0 t / ha. Protein content – 20.9 %, cellulose – 21.4 %. The first slope is 54–55 days. Clearness – 48 %. Conclusions. The quality of Rosana's fodder and seed yield is significantly higher than the Regina variety and the conditional standard. Characterized by a number of other economic and valuable features. It is recommended for distribution in Polissya and Forest-steppe zones of Ukraine in clean and mixed crops with other perennial herbs for the production of high-quality feed (haylage, hay, herbal pellets).
Keywords: alfalfa crop, varietal, winter resistance, dry matter, seeds.
Activity of antioxidant enzymes in chloroplasts of transgenic plants of wheat with double-stranded RNA-suppressor of the prolindehydrogenase gene
Authors: Kulesh S. S. , Dubrovna O. V. , Sokolovska-Serhiienko O. G. , Kiriziy D. A. , Priadkina G. O. , Stasik O. O. 
Published: March 2020

Aim. To investigate the activity of antioxidant enzymes in chloroplasts of transgenic wheat plants variety Zymoyarka containing a double-stranded RNA-suppressor of the proline dehydrogenase gene. Methods. Spectrophotometrical determination of the activity of enzymes. Results. Under physiological conditions, the activity of superoxide dismutase (SOD) and ascorbate peroxidase (APX) in transgenic plants significantly exceeded these indices in non-transgenic forms. Under soil drought, the activity of SOD in transgenic plants decreased significantly and amounted to only 70 % of the activity of this enzyme in control plants, the activity of APX was 12 % lower than that of control. The water deficit increased sharply (4–5 times) the level of free L-proline in transgenic wheat plants with reduced activity pdh. Conclusions. Biotechnological plants of wheat with increased activity of SOD and APX under optimum conditions can be characterized by increased adaptive plasticity compared to plants of original variety with lower activity of the enzymes. The increased activity of antioxidant enzymes may be a prerequisite for increasing the tolerance of these plants to stressors of different origins.
Keywords: Triticum aestivum L., transgenic plants, double-stranded RNA-suppressor of the proline dehydrogenase gene, L-proline, antioxidant enzymes.
Genetic markers of moldovan tsigayand karakul sheep
Authors: Lyutskanov P. I. , Mashner O. A. , Marzanov N. S. 
Published: March 2020

Aim. The study of the genetic structure of the two created Moldovan types of sheep – Tsigay wool-meat-dairy and karakul astrakhan -meat-and-milk types by the frequency of antigens, genotypes, alleles, homozygosity level and the number of effective alleles. Methods. Genetic analysis was performed using seven blood group systems (A, B, C, D, M, R, I) by the method of setting the hemolysis and agglutination reactions. Results. At MoldovanTsigay sheep, the frequency of antigens in blood group systems varies from 0.05 to 0.95. There are 38 genotypes and 20 alleles. The total homozygosity constituted 0.5393 and the efficacy per allele was 1.866. In contrast to the Tsigay sheep the Moldovan karakul sheep have 136 genotypes and 48 alleles, the total homozygosity is the same – 0.5230, and the efficiency per allele is almost twice higher – 3.3574. Conclusions.Genetic markers are useful in an objective assessment of genetic diversity and the degree of kinship of sheep breeds. Being an integral part of the genofondof the population, they provide valuable information about the changes occurring in it in the breeding process.
Keywords: Sheep, antigens, genotypes, alleles, homozygosity.
Common and specific gene signatures among three different endometriosis subtypes
Authors: Jiang Li , Zhang Mengmeng , Wang Sixue , Han Yuanyuan , Fang Xiaoling 
Published: March 2020

AimsTo identify the common and specific molecular mechanisms of three well-defined subtypes of endometriosis (EMs): ovarian endometriosis (OE), peritoneal endometriosis (PE), and deep infiltrating endometriosis (DIE).MethodsFour microarray datasets:GSE7305andGSE7307for OE,E-MTAB-694for PE, andGSE25628for DIE were downloaded from public databases and conducted to compare ectopic lesions (EC) with eutopic endometrium (EU) from EMs patients. Differentially expressed genes (DEGs) identified by limma package were divided into two parts: common DEGs among three subtypes and specific DEGs in each subtype, both of which were subsequently performed with the Kyoto Encyclopedia of Genes (KEGG) pathway enrichment analysis. The protein-protein interaction (PPI) network was constructed by common DEGs and five hub genes were screened out from the PPI network. Besides, these five hub genes together with selected interested pathway-related genes were further validated in an independent OE RNA-sequencing datasetGSE105764.ResultsA total of 54 EC samples from three EMs subtypes (OE, PE, DIE) and 58 EU samples were analyzed, from which we obtained 148 common DEGs among three subtypes, and 729 specific DEGs in OE, 777 specific DEGs in PE and 36 specific DEGs in DIE. The most enriched pathway of 148 shared DEGs was arachidonic acid (AA) metabolism, in which most genes were up-regulated in EC, indicating inflammation was the most common pathogenesis of three subtypes. Besides, five hub genes AURKB, RRM2, DTL, CCNB1, CCNB2 identified from the PPI network constructed by 148 shared DEGs were all associated with cell cycle and mitosis, and down-regulated in EC, suggesting a slow and controlled proliferation in ectopic lesions. The KEGG pathway analysis of specific DEGs in each subtype revealed that abnormal ovarian steroidogenesis was a prominent feature in OE; OE and DIE seems to be at more risk of malignant development since both of their specific DEGs were enriched in the pathways in cancer, though enriched genes were different, while PE tended to be more associated with dysregulated peritoneal immune and inflammatory microenvironment.ConclusionBy integrated bioinformatic analysis, we explored common and specific molecular signatures among different subtypes of endometriosis: activated arachidonic acid (AA) metabolism-related inflammatory process and a slow and controlled proliferation in ectopic lesions were common features in OE, PE and DIE; OE and DIE seemed to be at more risk of malignant development while PE tended to be more associated with dysregulated peritoneal immune and inflammatory microenvironment, all of which could deepen our perception of endometriosis.
Sanitary quality in seeds from species of Caatinga biome and control methods for fungi
Authors: Nascimento Luan Vítor , Nogueira Geovane de Almeida , Costa Alves Tatianne Raianne , Medeiros Araújo Maria Bruna , Dombroski Jeferson Luiz Dallabona , Machado Frederico Santos , Ambrósio Márcia Michelle de Queiroz 
Published: March 2020

Sanitary and quality of seeds of three native species (Aroeira, Jucá, and Mofumbo) from the Caatinga biome, Brazil, and different control methods of microorganisms in seeds were evaluated. We used 500 seeds of each species, 100 for each treatment: control, superficial disinfestation, Enzimatic II, Captana and Mancozebe. Seeds were distributed in Petri dishes containing potato-dextrose-agar culture medium and stored in incubator type Biochemical Oxygen Demand at 28 ± 2 °C for five days. For germination tests, 100 seeds were arranged in germitest paper previously sterilized and moistened with sterilized distilled water, and incubated for 19 (mofumbo), 20 (jucá), and 25 (aroeira) days in incubators at 28 ± 2 °C. Aspergillus flavus, A. niger, A. ochraceus, Lasiodiplodia sp., Penicillium sp. and Rhizopus sp. are associated with the studied seeds. Captana reduced the incidence of Aspergillus and Penicillium in the seeds, being the most efficient in controlling the microfauna associated with these seeds. Enzimatic II also proved to be an efficient control for Aspergillus spp. There was no interference of the tested products on seed germination.