bluuebunny/crossref_metadata_2025_split huggingface dataset:
Computational design of ancestral and consensus sequence of apical membrane antigen 1 (AMA1) of Plasmodium spp
Authors: Nurdiansyah Rizky , Kemal Rahmat Azhari 
Published: February 2020

Background: It is important to design a malaria vaccine targeting all human malaria parasites as well as non-human primate parasites to eradicate malaria and prevent zoonotic malaria. Apical membrane antigen 1 (AMA1) protein is shared by human-infecting Plasmodium species. Ancestral sequence reconstruction (ASR) and consensus sequence construction on AMA1 might be able to overcome the antigenic distinction between those species.
Objective: We aimed to computationally design the ancestral and consensus sequence of Plasmodium AMA1 protein and analyze the sequences for its putative immunogenicity.
Methods: We utilized bioinformatics software to computationally design ancestral and consensus sequences of AMA1 protein. AMA1 protein sequences of human-infecting Plasmodium and non-human primate Plasmodium were retrieved from PlasmoDB. ASR was designed using MEGA X while consensus was inferred using UGENE. Phylogenetic tree consisting of existing Plasmodium sequences and the ancestral sequence was constructed using IQTREE webserver and visualized with FigTree.
Results: Phylogenetic analysis showed that Plasmodium spp. were divided into 2 major groups, P. falciparum (Clade F) and non-falciparum (Clade NF) thus three ancestral and consensus sequences were designed based on each clade and both clades at once. Reconstructed ancestral sequences were located as sister branch for naturally occurring strains. On the contrary, consensus sequences are located within the branch of corresponding naturally occurring strains. Sequence analysis showed the presence of CD8+ T cell epitope in all computationally-designed sequences.
Conclusion: Ancestral and consensus AMA1 sequences are potential for further studies as a malaria vaccine candidate.
Role of malondialdehyde (MDA) in patients with breast cancer diseases
Authors: Yerizel Eti , Astria Niki , Khambri Daan 
Published: February 2020

Background: Breast cancer is one of the most common cancers as well as one of the leading causes of cancer mortality in women worldwide. Cancer risk potentially continues to increase because of the many sources of exposure to carcinogenic chemical compounds. Carcinogenic compounds can contribute to free radical formation which might further interact and damage biomolecules such as lipids. Lipid peroxidation will increase malondialdehyde (MDA) levels, triggering gene mutations that leads to cancer.
Objective: The purpose of this research was to measure and compare MDA levels between breast cancer patients and control.
Methods: This research was observational research using a cross-sectional comparative design of 30 breast cancer patients and 30 healthy controls. The place of this research is in Ropanasuri specialized surgery hospital and biochemical laboratory, Faculty of Medicine, Andalas University, Padang. This research was conducted from August to September 2019. The MDA was measured using a spectrophotometer and an independent T-test was done.
Results: The result of this research showed the mean MDA level of breast cancer patients was 3.98 ± 0.35 nmol/ml, higher than controls was 3.04 ± 0.36 nmol/ml with p-value = 0.001.
Conclusion: There were significant differences in MDA levels among breast cancer patients and control in Ropanasuri specialized surgery hospital, Padang.
Alanine amino transferase (ALT) specific activities in long term systemic hypoxic rat brain tissues
Authors: Ramadhani Rizka , Prijanti Ani Retno 
Published: February 2020

Background: Brain as a very aerobic organ is sensitive to hypoxia. Energy scarcities must be overcome by gluconeogenesis, which uses alanine or lactate as starting material. The reaction is catalyzed by alanine amino transaminase (ALAT or ALT), also known as glutamate pyruvate transaminase (GPT).
Objective: To investigate whether the specific activities of alanine aminotransferase (ALT) increased in hypoxic rat brain.
Methods: This experimental study used rats exposed to systemic normobaric hypoxia during 14 days. A group of 5 rats was sacrificed in days 1, 3, 7 and 14. The specific activities of ALT were analyzed in their brains using a reaction coupled with lactate dehydrogenase (LDH) activities.
Results: The ALT specific activities in rat brain were very low. There was no significant increase of specific activities during long term hypoxia (p > 0.05).
Conclusion: The rat brain ALT has no role in gluconeogenesis.
“Eu era completamente saudável…”: um caso de consentimento desinformado
Authors: Silva Margarida Baldaque , Nogueira Teixeira Joana , Pires Rosa 
Published: February 2020

Descrição do caso:Homem de 60 anos, psicólogo. Antecedentes pessoais e familiares irrelevantes. Sem medicação habitual. Assintomático.Na consulta anual de Medicina do Trabalho, foi-lhe pedido estudo analítico com PSA. Por elevação do PSA, foi encaminhado para consulta de Urologia. Aí realizou biópsia prostática, cujo resultado foi inconclusivo.Um mês depois, recorre ao SU por febre, hematúria macroscópica e artralgias migratórias. Diagnosticada prostatite com artrite reativa e lesão renal aguda (atribuída ao consumo de AINEs). Iniciou ciprofloxacina (urocultura estéril). O estudo analítico efetuado mostrou anemia, elevação dos marcadores inflamatórios e ANAs positivos. Na reavaliação em consulta de Medicina Interna, mantinha poliartralgias e referia náuseas, epigastralgia e anorexia, com perda de 10% do peso corporal, bem como anedonia, apatia, preocupação e insónia. Foi pedido novo estudo analítico, EDA, TC-TAP, orientado para consulta de Doenças Auto-Imunes e medicado com domperidona e trazodona.Por agravamento da função renal, foi convocado para internamento, onde ocorreu melhoria espontânea da mesma. Manteve anemia com necessidade de transfusão. Iniciou corticosteróide e ciclosfosfamida. Realizou biópsia renal e RMN abdominal (para caraterização de micronódulos hepáticos encontrados na TC, mas foi inconclusiva devido à impossibilidade de administrar contraste dada a função renal). Teve alta com o diagnóstico de poliangeíte microscópica e medicado com epoetina, prednisolona, cotrimoxazol, nistatina e ranitidina. Ficou com sessões em Hospital de Dia agendadas durante 5 meses e foi encaminhado para consulta de Nefrologia. Comentário:Este caso constitui um exemplo de sobrediagnóstico (deteção clínica de patologias que sem investigação médica permaneceriam silenciosas e nunca derivariam num quadro sintomático ou em morte). Como fica demonstrado, a solicitação do PSA sérico pode iniciar uma cadeia diagnóstica danosa para o doente. Recomenda-se uma decisão partilhada e informada entre o médico e o utente. Palavras-chave: PSA, consentimento informado, poliangeíte microscópica.
Uma improvável queimadura: Relato de caso de Esclerose Sistémica Difusa
Authors: Dias Rosa Catarina Isabel 
Published: February 2020

Introdução:A Esclerose Sistémica Difusa (ESD) é uma doença autoimune, de causa desconhecida, que pode afetar múltiplos sistemas de órgãos, condicionando gravemente a qualidade de vida dos doentes afetados. Embora não sejam conhecidos os agentes que modifiquem a evolução da doença, a terapêutica imunossupressora deverá ser iniciada numa fase precoce, sendo por esse motivo primordial o seu reconhecimento nas fases mais iniciais. Inevitavelmente a ESD condiciona elevado impacto a nível físico e psicológico do doente, devendo ser considerados fatores como o estado nutricional, a dor, o descondicionamento físico, a atrofia muscular, comorbilidades, o impacto psicológico da doença e o isolamento social. A abordagem dos doentes com ESD deve ser por isso baseada numa visão global e multidisciplinar. Descrição do caso:Apresenta-se um caso de uma mulher de 50 anos, sem antecedentes pessoais ou familiares de relevo para a doença atual. Apresentava edema e rubor de ambas as mãos associado a rigidez matinal de 30 minutos com duas semanas de evolução, sendo objetivável ao exame objetivo edema em todos os dedos de ambas as mãos, rubor ligeiro nas articulações metacarpicofalâncias, interfalângicas distais e interfalângicas proximais, ausência de dor à palpação ou mobilização das articulações e ligeira limitação na flexão. Foi orientada a nível hospitalar inicialmente pela especialidade de Medicina Interna tendo sido colocada a hipótese diagnóstica de ESD após realização de meios complementares de diagnóstico. Por se tratar de uma doença que multiorgânica mantem seguimento atualmente em consultas de Reumatologia, Dermatologia, Medicina Física e de Reabilitação e Pneumologia. Comentário:Embora a ESD seja uma doença extremamente rara, o relato deste caso realça a importância do conhecimento por parte do médico de família dos sinais de alarme da doença reumatológica. Alem disso o médico de família tem ainda papel na gestão da multimorbilidade associada e de suporte aos cuidadores do doente.
Empatia e arte – poderá um programa de arte numa unidade de saúde familiar influenciar a empatia percecionada pelos utentes?
Authors: Tomaz Teresa , Neves Ana Marta , Barbosa Benvinda , Fachado Francisco , Fonte Pedro 
Published: February 2020

Introdução: A empatia é uma técnica essencial na relação clínica, podendo ser treinada e aferida. Pensa-se que a exposição dos profissionais de saúde a programas de arte possa melhorar a empatia clínica percecionada pelos utentes; porém, desconhece-se qual a sua influência na comunicação com os utentes. Objetivos: Avaliar a influência dum programa de arte na empatia percecionada pelos utentes relativamente aos profissionais de saúde duma unidade de saúde familiar e verificar a relação entre a empatia percecionada pelos utentes e as características sociodemográficas dos mesmos. Métodos: Estudo quase-experimental, com avaliação pré e pós-intervenção, conduzido numa unidade de saúde familiar entre janeiro e julho de 2018. Utilizaram-se amostras de conveniência calculadas a partir da população inscrita na unidade de saúde familiar com idade igual ou superior a 18 anos. A intervenção, aplicada aos médicos e enfermeiros, consistiu num programa de arte constituído por três sessões referentes a cinema, literatura e fotografia. Para determinar a influência da intervenção, avaliou-se a empatia pré e pós-intervenção através do questionário “The Consultation and Relational Empathy” e dum questionário sociodemográfico. As associações entre variáveis foram testadas com testes não paramétricos (Mann-Whitney e Kruskal-Wallis) e correlação de Spearman, com um nível de significância de 0,05.Resultados: Obtiveram-se 390 e 371 questionários antes e após a intervenção. Verificou-se um aumento estatisticamente significativo da empatia percecionada pelos utentes após a intervenção (p=0,001). As únicas associações estatisticamente significativas verificadas foram com a escolaridade (p=0,000) e situação profissional (p=0,0001) no período pré-intervenção.Discussão: Demonstra-se que um programa de arte aplicado a uma equipa de saúde pode apresentar impacto na empatia percecionada pelos utentes, alertando para a possibilidade de criação futura de espaços de integração artística orientados para o ensino da empatia clínica nos cuidados de saúde primários.
PENGARUH PENAMBAHAN SUMBER PROTEIN NABATI BUNGKIL KELAPA TERHADAP PERTAMBAHAN BOBOT BADAN TERNAK KAMBING PERANAKAN ETTAWA (PE)
Authors: Sarimo Hariyati 
Published: February 2020

Hariyati Sarimo. 621415025. Effect of Addition of Coconut Vegetable Protein Source to the Increase in Weight of Ettawa (PE) Goat Breeding Body. Department of Animal Husbandry, Faculty of Agriculture, Gorontalo State University. Guided by Nibras Karnain Laya and Umbang Arif Rokhayati. The study aimed to determine body weight gain and feed conversion. This research was conducted in March 2019 - April 2019. The location of the study was conducted in Paris Village, Mootilango District, Gorontalo Regency. The study used 20 female ettawa (PE) Peranakan goats with age 2-3 years by providing forage feed and 50% coconut cake added. While processing data using the t test. The results showed that the addition of coconut cake was significantly different (P 0.05) to the value of the weight gain of ettawa goat breeds. where the average increase in body weight of ettawa Peranakan goat (PE) is 33 g / head / day. For feed conversion in ettawa (PE) crossbreed goats who were given an additional feed of coconut cake showed feed conversion of 0.18% and Etawa Peranakan goats given forage feed alone had a feed conversion value of 0.27%. The conclusion is that the addition of coconut cake was significantly different (P 0.05) on the increase in Ettawa (PE) goat body weight gain.
IMPLEMENTASI PEMBELAJARAN BERBASIS SISTEM KREDIT SEMESTER (SKS) DI SMA NEGERI 2 BALIKPAPAN TAHUN AJARAN 2018/2019
Authors: Hardiana Trianingtyas Novri , Casmudi Casmudi , Indriawati Prita 
Published: February 2020

Tujuan penelitian ini untuk mendeskripsikan implemantasi pembelajaran berbasis sistem kredit semester di SMA Negeri 2 Balikpapan. Jenis penelitian dengan pendekatan kualitatif. Sumber data berupa data primer dan sekunder. Teknik pengumpulan data dengan observasi, wawancara, dan dokumentasi. Teknik analisis data dengan menggunakan teknik analisis data model interaktif (Miles dan Huberman). Pengecekan keabsahan data dengan menggunakan teknik triangulasi. Hasil penelitian ini menyimpulkan bahwa: 1) SMA negeri 2 Balikpapan menerapkan 7 prinsip penyelenggaran Sistem Kredit Semester, 2) Mekanisme penyelenggaraan Sistem Kredit Semester terdapat 3 tahapan yakni tahapan persiapan, tahapan pelaksanaan dan terhapan evaluasi serta monitoring. 3) SMA Negeri 2 Balikpapan telah melaksanakan persiapan pelaksanaan SKS secara baik dan matang, dan pada dasarnya pengimplementasian SKS sudah terlaksana dengan baik, namun belum terlaksana secara maksimal disebabkan terdapat berbagai kendala pengimplementasian dalam proses pembelajaran, 4) Upaya atau solusi yang dapat di ambil sekolah untuk mengatasi kendala yang terjadi dengan memberikan pelatihan mengenai IT pada guru yang kurang dan In house traning mengenai penerapan Sistem Kredit Semester.
An effective approach for botulinum toxin injection in patients with stroke for focal spasticity: dual guidance
Authors: Ata Emre , Kosem Murat 
Published: February 2020

Objective: This study has aimed to compare the efficacy of botulinum toxin (BTX) injections, applied to the upper limb muscles of the stroke patients in our clinic who have being diagnosed with focal spasticity, that are performed via ultrasonography and ultrasonography + electrical muscle (EM) stimulator guidance. Literature didn’t include detailed crosscheck of ultrasound guided BTX and EM treatment applications for upper limb focal spasticity patients
Materials and Methods: Electronic data on 62 hemiplegic stroke patients with grade 2 and 3 focal spasticity who had received botulinum toxin injections into their upper limb muscles by the same physician, who used similar protocol and recorded the results, were scanned retrospectively. The spasticity of the patients in both groups was assessed with the Modified Ashworth Scale at the end of two weeks and three months.
Results: A statistically significant difference was found between the Modified Ashworth Scale values of both groups in terms of all muscles, compared to the values seen in the pre-treatment period (p<0.05). The Modified Ashworth Scale values at 3 months posttreatment in ultrasonography + electrical muscle stimulator group were not statistically different from those at 2 weeks posttreatment, with respect to wrist flexion and finger flexion. In intergroup comparison, there was no statistically significant difference between the Modified Ashworth Scale values of at pretreatment and 2 weeks posttreatment. However, statistically significant difference in all muscle groups was found in favor of the ultrasonography + electrical muscle stimulator group at 3 months posttreatment controls (p<0.05).
Conclusion: Upper limb spasticity due to stroke can be substantially recovered with botulinum toxin injections that are applied via only ultrasonography guidance or via ultrasonography + electrical muscle stimulator guidance.. According to data from the assessment at 3 months posttreatment, the botulinum toxin injection performed via ultrasonography + electrical muscle stimulator guidance had more positive effects .
SISTEM PENDUKUNG KEPUTUSAN MENENTUKAN HASIL BUDIDAYA UDANG VANAME DENGAN METODE ALGORITMA C4.5 (PT ANUGERAH SUMBER LAUT JAYA)
Authors: Iqbal Muchamad , Usino Wendi , Triono Triono 
Published: February 2020

Abstrak - Kondisi geografis Indonesia dengan dua per tiganya perairan dapat memberikan keuntungan tersendiri bagi masyarakat Indonesia yang berprofesi sebagai nelayan. Salah satu komoditas budidaya air laut yang memiliki nilai ekonomi dan permintaan ekspor yang tinggi salah satunya adalah udang vaname (Liopenaeus Vannamei), yang sering disebut sebagai udang putih. Salah satu metode klasifikasi adalah algoritma C4.5, metode algoritma C4.5 dalam pemilihan atribut dapat dilakukan dengan cara menggunakan Gain dengan tujuan mencari nilai Entropy. Algoritma C4.5 dapat membagi-bagi data berdasarkan kriteria yang dipilih agar terbentuk sebuah pohon keputusan (Decision Tree) yang menggunakan pendekatan secara top down. Dari data-data yang diperoleh maka bisa dihitung dari hasil perhitungan pada luas tambak, bibit, pakan, dan pupuk akan didapatkan hasil optimasinya. Hasil dari program yang dibuat berdasarkan data-data kualitas air yang dianalisa dengan teknik data mining dapat dimanfaatkan untuk mendapatkan informasi yang berguna untuk pihak perusahaan dan dari pemodelan pohon keputusan dengan metode Algoritma C4.5 didapatkan hasil accuracy sebesar 76,47%, precision sebesar 72,72% dan recall sebesar 88,88%. Dari hasil pengujian tersebut diketahui bahwa klasifikasi termasuk dalam kategori Fair Classification.
Abstract - Indonesia's geographical condition, with two-by-one of its waters, can provide its own benefits for Indonesians who are fishermen. One of the sea water cultivation commodities which has economic value and high export demand one of them is shrimp Vaname (Liopenaeus Vannamei), which is often referred to as white shrimp. One method of classification is algorithm C 4.5, algorithm method C 4.5 in attribute selection can be done by using Gain with the purpose of finding the value of Entropy. The C 4.5 algorithm can divide data based on criteria chosen to form a decision Tree using a top down approach. From the data obtained, it can be calculated from the results of calculations on the area of pond, seedlings, feed, and fertilizer will be obtained the results of its optimization. The results of the programs created based on water quality data analyzed by data mining techniques can be utilized to obtain useful information for the company and from the modeling of decision tree with algorithm method C 4.5 Obtained the accuracy of 76.47%, precision at 72.72% and recall by 88.88%. From the test results it is known that the classification belongs to the Fair Classification category.
IMPLEMENTASI METODE K-MEANS DALAM PENGKLASTERAN DAERAH PUNGUTAN LIAR DI KABUPATEN SUKABUMI (STUDI KASUS : DINAS KEPENDUDUKAN DAN PENCATATAN SIPIL)
Authors: Sembiring Falentino , Octaviana Octaviana , Saepudin Sudin 
Published: February 2020

Abstrak - Pungutan liar (pungli) merupakan tindakan kejahatan yang dilakukan oleh pihak yang tidak bertanggung jawab atau seseorang atau pegawai negeri atau pejabat negara dengan cara meminta pembayaran uang yang tidak termasuk kedalam aturan administrasi yang dibutuhkan. Metode K-Means dapat membantu mengklasifikasikan daerah pungutan liar di kabupaten Sukabumi pada dinas kependudukan dan pencatatan sipil. Variabel yang digunakan dalam penelitian ini E-KTP, AKTA dan Kartu Keluarga Setiap variabel memiliki nilai bobot yang berbeda. Data yang akan digunakan dalam penelitian ini adalah data laporan pada bulan Januari 2019 berbentuk SQL. Penelitian ini hanya akan membahas tentang berapa banyak kejadian pungli yang terjadi di setiap kecamatan yang ada di kabupaten Sukabumi tentang kependudukan dan pencatatan sipil. Adapun tujuan dari penelitian ini adalah untuk menentukan cluster tingkat pungutan liar tinggi, sedang dan rendah. Hasil dari penelitian ini memperoleh data indeks kecamatan dengan tingkat jumlah laporan masyarakat daerah terhadap pungutan liar, data dengan klasifikasi tingkat tinggi yaitu Cireunghas, Gegerbitung, Kalapa Nunggal, Kalibunder, Purabaya, Simpenan, Parung Kuda, Sukaraja, Nagrak, Nyalindung, Pelabuhanratu, Surade, Warungkiara. Data tingkat pungutan liar sedang terdapat pada 19 kecamatan dan 15 tingkat pungutan liar rendah. Hal ini dapat menjadi masukan kepada dinas kependudukan dan pencatatan sipil, kecamatan yang menjadi tindakan pungutan liar paling tinggi diprioritas untuk melakukan penyuluhan kepada kecamatan tersebut.
Abstract - Illegal Levies is a criminal offensse done by someone or civil servant and the government by demanding a specific amount of money outside the right protocols or policy that does not related with the official administration fees. K-Means method will be able to help for classifying the illegal levies area in sukabumi district at The Department of Population and Civil Registration. Variables that used in this research are electronic ID card, birth certificate and family card. Meanwhile, data that will be used in this research are report data on January 2016. The purpose of this research is to determine the rate of illegal levies. The result that have been collected based on the high extortion rate from each sub-districts are Cireunghas, Gegerbitung, Kalapa Nunggal, Kalibunder, Purabaya, Simpenan, Parung Kuda, Sukaraja, Nagrak, Nyalindung, Pelabuhanratu, Surade, Warungkiara, 19 sub-districts with medium rate of illegal levies and 15 sub-district with low rate of illegal levies. This can become a recommendation for The Department of Population and Civil Registration, sub-districts with the highest rate of illegal levies will be prioritized in counseling for the certrain sub-districts.