bluuebunny/crossref_metadata_2025_split huggingface dataset:
Distribution and Conservation Status of the Crayfish Fauna of South Carolina, USA
Authors: Brown Bryan L. , Turnbull Matthew , Skelton James , Creed Robert P. 
Published: April 2020

Abstract
Recent studies have revealed integral and complex relationships between hosts and their symbionts. Some of these findings demonstrate that symbionts can affect the gene expression of their hosts. We used a model cleaning symbiosis system of crayfish and their branchiobdellidan symbionts to examine whether symbionts could affect host gene expression through indirect means, by changing the interaction of the host crayfish with the environment. Previous research has shown this symbiosis to be a complex, context dependent relationship in which outcomes can shift between mutualism, when branchiobdellidans act as cleaners of their hosts, and parasitism, when damage to hosts’ gills through branchiobdellidan feeding produces negative effects. These shifts are known to occur with changes in symbiont densities and environmental conditions. We manipulated densities of branchiobdellidans on host crayfish and examined the effect on expression of 3 candidate immune genes. We had 2 competing working hypotheses: 1) That branchiobdellidans would cause an increase expression in immune genes because of damage to the hosts’ gills; 2) That branchiobdellidans would decrease expression of immune genes by acting as cleaners and thus alleviating environmental stress. We found that the second hypothesis was strongly supported when branchiobdellidan activity decreased expression in 2 of the 3 candidate genes. One of these genes (astacidin) was related to generalized immune defense, while the other (prophenoloxidase) is a key part of the melanization cascade that provides defense in the case of physical damage and intrusion of microbes or foreign bodies. Given that there is no known mechanism through which branchiobdellidans can directly affect host physiology through chemical or genetic interactions, we concluded that this alteration in gene expression occurred through alleviation of environmental stress by the symbionts. Such indirect effects may be common in nature and continued study using easily manipulated systems like the crayfish-branchiobdellidan symbiosis may continue to produce insights regarding the importance of symbioses in ecological systems.
Optimism and happiness in undergraduate students: Cognitive flexibility and adjustment to university life as mediators
Authors: Demirtaş Ayşe Sibel 
Published: April 2020

De acuerdo con el objetivo del enfoque de psicología positiva, este estudio fue diseñado para construir un modelo de mediación múltiple que permita comprender mejor las relaciones entre optimismo, flexibilidad cognitiva, ajuste a la vida universitaria y felicidad. Un total de 386 estudiantes universitarios (64% mujeres y 36% hombres) entre 18-22 años participaron en el estudio. Los participantes fueron reclutados de una pequeña universidad en Turquía. El Test de Orientación de Vida, el Inventario de Flexibilidad Cognitiva, la Escala de Vida Universitaria y el Formulario Corto del Cuestionario de Felicidad de Oxford fueron utilizados como medidas. Los resultados mostraron que el optimismo, la flexibilidad cognitiva, el ajuste a la vida universitaria y la felicidad correlacionan positivamente. Los resultados de los análisis mediacionales también indicaron que la mediación múltiple en serie de la flexibilidad cognitiva y el ajuste a la vida universitaria en el modelo hipotético fue estadísticamente significativa y explicó aproximadamente el 50% de la varianza de la felicidad.
In accordance with the goal of the positive psychology approach, this study was designed to build an understanding of the relationships among optimism, cognitive flexibility, adjustment to university life and happiness by proposing a multiple mediation model. A total of 386 undergraduates (64% female and 36% male) between 18-22 years participated in the study. The participants were recruited from a small university in Turkey. The Life Orientation Test, Cognitive Flexibility Inventory, University Life Scale, and Oxford Happiness Questionnaire Short Form were utilized as measures. The results showed that optimism, cognitive flexibility, adjustment to university life, and happiness positively correlated. The findings from the mediational analyses also indicated that the serial multiple mediation of cognitive flexibility and adjustment to university life in the hypothetical model was statistically significant and explained approximately 50% of the variance in happiness.
Impact of Application of Fertilizer and Lime on Yield of Banana (Grand Naine) and Soil Parameters in Acidic Soil of Arunachal Pradesh
Authors: Kumar Jitendra , Kalita H. , Angami Thejangulie , Ramajayam D. , Sen Amit , Shukla Kshtiz 
Published: May 2020

The present study was aimed at standardizing fertilizer and lime requirement for tissue banana (Grand Naine) cultivation in acidic soil of mid hill of Arunachal Pradesh The experiment was conducted at ICAR RC farm Gori, ICAR RC for NEH Region, AP centre, Basar. The effect of twelve different treatments of combination of fertilizers and Lime was studied on growth parameters of banana plant, post harvest fruit quality parameters and on the soil chemical and physical parameter their availability & uptake of major nutrients were also studied. The results revealed. Plant growth parameters like pseudo stem circumference (36.96 cm) and Plant height (92.85 cm) was highest in the treatment receiving L1F2. Similarly, treatment L2F2 recorded highest number of leaves (5.96), suckers (1.41) and leaf area (3586 cm2). Treatment L2F1 recorded lowest stem circumference (22.3 cm), number of leaves (3.52) and leaf area (1420 cm2). Similarly, treatment having L3F1 recorded lowest number of suckers (0.41). The plants treated with (50% lime + 100% RDF) performed best in terms of both yield and quality attributes. The effects of lime application significantly improve pH of soil that resulted in the improved the availability of phosphorous. Application of 125% fertilizer was not found to significantly enhance the yield of Banana. The lime application significantly improve the soil nutrient concentration Viz Available N, P K, Ca pH and reduced Al concentration that resulted into enhance banana production compare to the control.
Impact of interfractional anatomical variation and setup correction methods on interfractional dose variation in IMPT and VMAT plans for pancreatic cancer patients: A planning study
Authors: Ashida Ryo , Nakamura Mitsuhiro , Yoshimura Michio , Mizowaki Takashi 
Published: April 2020

AbstractPurposeTo investigate the impact of interfractional anatomical changes and setup correction methods on dose distributions in pancreatic cancer patients under breath‐hold conditions.MethodsThree intensity‐modulated proton therapy (IMPT) plans with different beam arrangements and one volumetric‐modulated arc therapy (VMAT) plan prescribing 54 Gy in 30 fractions were created for 10 patients who underwent three additional CT scans performed at an interval of 1–2 weeks. The additional CT sets were rigidly registered to the simulation CT set using both bone‐matching (BM) and organ‐matching (OM) methods in each patient. Recalculated dose distributions and dose–volume indices on the additional CT sets using either the BM or the OM method were compared with the simulation values.ResultsDifferences in the gross tumor volume D98% value from the simulation sets ranged from −0.8 to −5.9% on average. In addition, the variations were larger with OM compared with BM for two IMPT plans. Meanwhile, differences in the D98% value in the region isotropically enlarged by 5 mm from the gross tumor volume were significantly improved with OM on two IMPT plans and the VMAT plan. Among the organs at risk, the dose–volume indices were significantly improved with OM only in the duodenum on all plans.ConclusionOrgan‐matching may be a better setup correction technique than BM for both photon therapy and IMPT plans. However, in some beam arrangements of IMPT, the dose distribution may be somewhat worse using OM, due to interfractional anatomical variation. Therefore, it is important to choose beam angles that are less likely to be influenced by changes in the gastrointestinal gas volume, especially in IMPT plans.
Analysis of factors associated with brittle response in patients with Parkinson’s disease
Authors: Yan Yayun , Li Yanyan , Liu Xiufeng , Zhang Liyao , Wang Lu , Chang Ying 
Published: April 2020

AbstractIntroductionThe brittle response (BR) in patients with Parkinson’s disease (PD) refers to a special type of levodopa‐induced dyskinesia (LID). This study aimed to describe the clinical characteristics of BR patients and to analyze the associated risk factors.MethodsA retrospective study was conducted to analyze the data of 97 patients with PD. Patients were divided into a BR group and a non‐brittle response (NBR) group. Demographic and clinical data, motor symptoms, and non‐motor symptoms of the two groups were assessed.ResultsAmong 97 PD patients, 11 were in the BR group and 86 were in the NBR group. The proportion of female patients was 72.7% and 38.3%, respectively, in the BR and NBR groups (P < 0.05). Compared to NBR patients, BR patients had relatively low body weight, low BMI, long disease duration, high levodopa equivalent daily dosage (LEDD), and high levodopa dose per weight (P < 0.05). The BR group had significantly higher scores of UPDRS (II, III, and IV) (P < 0.05). But no difference was found in the UPDRS I, emotional state, cognitive status, and accompanied by REM sleep behavior disorder (RBD) (P> 0.05). Multivariate logistic regression analysis showed that BR patients had lower body weight and higher levodopa dose per weight.ConclusionBR is associated with being female, low body weight, low BMI, long disease duration, high LEDD, and high levodopa dose per weight. Body weight and levodopa dose per body weight are independent risk factors for BR.
Medical Cannabis Use by Rheumatology Patients Following Recreational Legalization: A Prospective Observational Study of 1000 Patients in Canada
Authors: Fitzcharles Mary‐Ann , Rampakakis Emmanouil , Sampalis John , Shir Yoram , Cohen Martin , Starr Michael , Häuser Winfried 
Published: April 2020

ObjectiveRecreational legalization of cannabis may influence the medical use by patients. When only medical access was legally available in Canada, 4.3% of rheumatology patients reported use. With the current recreational legalization, we have reexamined the prevalence and characteristics of medical cannabis use in this same rheumatology setting.MethodsConsecutively attending rheumatology patients participated in an onsite survey comprising the following two questionnaires: 1) demographic and disease information completed by the rheumatologist and 2) patient anonymous questionnaire of health status, cannabis use (recreational and/or medicinal), and characteristics of cannabis use.ResultsOf 1047 attendees from June to August 2019, with 1000 participating, medical cannabis had been used by 12.6% of patients (95% confidence interval 10.7%‐14.8%), with half continuing use for mostly pain relief. Discontinuation was due to lack of effect in 57% of patients and side effects in 28% of patients. Ever medical users were younger (61.2 vs. 64.9 years; P = 0.006), more likely unemployed/disabled (16.7% vs. 5.9%; P < 0.001), and had more previous (47.6% vs. 25.5%; P < 0.001) and current recreational cannabis use (17.5% vs. 3.1%; P < 0.001) than nonusers. Most patients used multiple methods of administration, including smoking, vaporizing, and using oral oil preparations, but were poorly knowledgeable of product content, which was bought solely via the legal medical route by only 20%, and only one‐third disclosed their use to the rheumatologist.ConclusionMedical cannabis use has tripled for rheumatology patients since recreational legalization, with users being younger, not working, and having recreational cannabis experience. Concerning issues are the poor knowledge of the product being used, access via the nonmedical route, and nondisclosure to the physician.
Incidence of Uveitis in Secukinumab‐treated Patients With Ankylosing Spondylitis: Pooled Data Analysis From Three Phase 3 Studies
Authors: Deodhar Atul A. , Miceli‐Richard Corine , Baraliakos Xenofon , Marzo‐Ortega Helena , Gladman Dafna D. , Blanco Ricardo , Das Gupta Ayan , Martin Ruvie , Safi Jorge , Porter Brian , Shete Abhijit , Rosenbaum James T. 
Published: April 2020

ObjectiveThe objective of this study was to report the incidence of uveitis in secukinumab‐treated patients with ankylosing spondylitis (AS) in a pooled analysis of three phase 3 trials (MEASURE 1‐3 [ClinicalTrials.gov identifiers NCT01358175, NCT01649375, NCT02008916]).MethodsAnalysis included pooled patient‐level data from all patients (N = 794) who received any dose (one or more) of secukinumab up to the last patient attending the week 156 study visit in MEASURE 1 and up to the week 156 visit in MEASURE 2 and the week 104 visit in MEASURE 3 for each patient. Postmarketing data were from the periodic safety update report. Incidence of uveitis is reported as the exposure‐adjusted incidence rate (EAIR) per 100 patient‐years of secukinumab exposure.ResultsOverall, 135 (17%) patients reported preexisting (but not active or ongoing) uveitis at baseline, and 589 (74.2%) patients were HLA antigen B27 positive. The EAIR for uveitis was 1.4 per 100 patient‐years over the entire treatment period. Among all cases of uveitis (n = 26), 14 (54%) were flares. The exposure‐adjusted reporting rate of uveitis in the postmarketing data (which included patients across the three approved indications of psoriasis, psoriatic arthritis, and AS) was 0.03 per 100 patient‐years based on cumulative secukinumab exposure of 96 054 patient‐years.ConclusionThe incidence rate of uveitis in secukinumab‐treated patients with active AS does not suggest an increased risk with secukinumab treatment.
Integrated Dual‐Mode Chromatography to Enrich Extracellular Vesicles from Plasma
Authors: Van Deun Jan , Jo Ala , Li Huiyan , Lin Hsing‐Ying , Weissleder Ralph , Im Hyungsoon , Lee Hakho 
Published: April 2020

AbstractPurifying extracellular vesicles (EVs) from complex biological fluids is a critical step in analyzing EVs molecularly. Plasma lipoprotein particles (LPPs) are a significant confounding factor as they outnumber EVs >104‐fold. Given their overlap in size, LPPs cannot be completely removed using standard size‐exclusion chromatography. Density‐based separation of LPPs can be applied but is impractical for routine use in clinical research and practice. Here a new separation approach, known as dual‐mode chromatography (DMC), capable of enriching plasma EVs, and depleting LPPs is reported. DMC conveniently integrates two orthogonal separation steps in a single column device: i) size exclusion to remove high‐density lipoproteins (HDLs) that are smaller than EVs; and ii) cation exchange to clear positively charged ApoB100‐containing LPPs, mostly (very) low‐density lipoproteins (V)LDLs, from negatively charged EVs. The strategy enables DMC to deplete most LPPs (>97% of HDLs and >99% of (V)LDLs) from human plasma, while retaining EVs (>30% of input). Furthermore, the two‐in‐one operation is fast (15 min per sample) and equipment‐free. With abundant LPPs removed, DMC‐prepared samples facilitate EV identification in imaging analyses and improve the accuracy for EV protein analysis.
Simple and Scalable Mechanochemical Synthesis of Noble Metal Catalysts with Single Atoms toward Highly Efficient Hydrogen Evolution
Authors: Jin Haiyan , Sultan Siraj , Ha Miran , Tiwari Jitendra N. , Kim Min Gyu , Kim Kwang S. 
Published: April 2020

AbstractDesigning a facile strategy to access active and atomically dispersed metallic catalysts are highly challenging for single atom catalysts (SACs). Herein, a simple and fast approach is demonstrated to construct Pt catalysts with single atoms in large quantity via ball milling Pt precursor and N‐doped carbon support (K2PtCl4@NC‐M; M denotes ball‐milling). The as‐prepared K2PtCl4@NC‐M only requires a low overpotential of 11 mV and exhibits 17‐fold enhanced mass activity for the electrochemical hydrogen evolution compared to commercial 20 wt% Pt/C. The superior hydrogen evolution reaction (HER) catalytic activity of K2PtCl4@NC‐M can be attributed to the generation of Pt single atoms, which improves the utilization efficiency of Pt atoms and the introduction of Pt‐N2C2 active sites with near‐zero hydrogen adsorption energy. This viable ball milling method is found to be universally applicable to the fabrication of other single metal atoms, for example, rhodium and ruthenium (such as Mt‐N2C2, where Mt denotes single metal atom). This strategy also provides a promising and practical avenue toward large‐scale energy storage and conversion application.
Layered Oxide Cathodes Promoted by Structure Modulation Technology for Sodium‐Ion Batteries
Authors: Xiao Yao , Abbasi Nasir Mahmood , Zhu Yan‐Fang , Li Shi , Tan Shuang‐Jie , Ling Wei , Peng Ling , Yang Tingqiang , Wang Lude , Guo Xiao‐Dong , Yin Ya‐Xia , Zhang Han , Guo Yu‐Guo 
Published: April 2020

AbstractConsidering the ever‐growing climatic degeneration, sustainable and renewable energy sources are needed to be effectively integrated into the grid through large‐scale electrochemical energy storage and conversion (EESC) technologies. With regard to their competent benefit in cost and sustainable supply of resource, room‐temperature sodium‐ion batteries (SIBs) have shown great promise in EESC, triumphing over other battery systems on the market. As one of the most fascinating cathode materials due to the simple synthesis process, large specific capacity, and high ionic conductivity, Na‐based layered transition metal oxide cathodes commonly suffer from the sluggish kinetics, multiphase evolution, poor air stability, and insufficient comprehensive performance, restricting their commercialization application. Here, this review summarizes the recent advances in layered oxide cathode materials for SIBs through different optimal structure modulation technologies, with an emphasis placed on strategies to boost Na+ kinetics and reduce the irreversible phase transition as well as enhance the store stability. Meanwhile, a thorough and in‐depth systematical investigation of the structure–function–property relationship is also discussed, and the challenges as well as opportunities for practical application electrode materials are sketched. The insights brought forward in this review can be considered as a guide for SIBs in next‐generation EESC.
Entrapping Digestive Enzymes with Engineered Mesoporous Silica Particles Reduces Metabolic Risk Factors in Humans
Authors: Waara Erik R. , Iqbal Muhammad N. , Robert‐Nicoud Ghislaine , Benziane Boubacar , Vallhov Helen , Wasik Agata M. , Lindgren Maria , Hagman Emilia , Rinde Mia , Kupferschmidt Natalia , Berlin Roger , Johnston Eric V. , Danielsson Pernilla , Bengtsson Tore 
Published: April 2020

AbstractEngineered mesoporous silica particles (MSP) are thermally and chemically stable porous materials composed of pure silica and have attracted attention for their potential biomedical applications. Oral intake of engineered MSP is shown to reduce body weight and adipose tissue in mice. Here, clinical data from a first‐in‐humans study in ten healthy individuals with obesity are reported, demonstrating a reduction in glycated hemoglobin (HbA1c) and low‐density lipoprotein cholesterol, which are well‐established metabolic and cardiovascular risk factors. In vitro investigations demonstrate sequestration of pancreatic α‐amylase and lipase in an MSP pore‐size dependent manner. Subsequent ex vivo experiments in conditions mimicking intestinal conditions and in vivo experiments in mice show a decrease in enzyme activity upon exposure to the engineered MSP, presumably by the same mechanism. Therefore, it is suggested that tailored MSP act by lowering the digestive enzyme availability in the small intestine, resulting in decreased digestion of macronutrient and leading to reduced caloric uptake. This novel MSP based mechanism‐of‐action, combined with its excellent safety in man, makes it a promising future agent for prevention and treatment of metabolic diseases.
An Ultrastrong and Highly Stretchable Polyurethane Elastomer Enabled by a Zipper‐Like Ring‐Sliding Effect
Authors: Shi Chen‐Yu , Zhang Qi , Yu Cheng‐Yuan , Rao Si‐Jia , Yang Shun , Tian He , Qu Da‐Hui 
Published: April 2020

AbstractElastomers with excellent mechanical properties are in substantial demand for various applications, but there is always a tradeoff between their mechanical strength and stretchability. For example, partially replacing strong covalent crosslinking by weak sacrificial bonds can enhance the stretchability but also usually decreases the mechanical strength. To surmount this inherent tradeoff, a supramolecular strategy of introducing a zipper‐like sliding‐ring mechanism in a hydrogen‐bond‐crosslinked polyurethane network is proposed. A very small amount (0.5 mol%) of an external additive (pseudo[2]rotaxane crosslinker) can dramatically increase both the mechanical strength and elongation of this polyurethane network by nearly one order of magnitude. Based on the investigation of the relationship between molecular structure and mechanical properties, this enhancement is attributable to a unique molecular‐level zipper‐like ring‐sliding motion, which efficiently dissipates mechanical work in the solvent‐free network. This research not only provides a distinct and general strategy for the construction of high‐performance elastomers but also paves the way for the practical application of artificial molecular machines toward solvent‐free polyurethane networks.
Facile Synthesis of Efficient Luminogens with AIE Features for Three‐Photon Fluorescence Imaging of the Brain through the Intact Skull
Authors: Qin Wei , Alifu Nuernisha , Lam Jacky W. Y. , Cui Yuhan , Su Huifang , Liang Guodong , Qian Jun , Tang Ben Zhong 
Published: April 2020

AbstractVisualization of the brain in its native environment is important for understanding common brain diseases. Herein, bright luminogens with remarkable aggregation‐induced emission (AIE) characteristics and high quantum yields of up to 42.6% in the solid state are synthesized through facile reaction routes. The synthesized molecule, namely BTF, shows ultrabright far‐red/near‐infrared emission and can be fabricated into AIE dots by a simple nanoprecipitation procedure. Due to their high brightness, large Stokes shift, good biocompatibility, satisfactory photostability, and large three‐photon absorption cross section, the AIE dots can be utilized as efficient fluorescent nanoprobes for in vivo brain vascular imaging through the intact skull by a three‐photon fluorescence microscopy imaging technique. This is the first example of using AIE dots for the visualization of the cerebral stroke process through the intact skull of a mouse with high penetration depth and good image contrast. Such good results are anticipated to open up a new venue in the development of efficient emitters with strong nonlinear optical effects for noninvasive bioimaging of living brain.