bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Effect of Lactofem Oral Probiotic Capsule on Lactobacilli Colonization and Some Vaginal Health Parameters
Authors: Balaghi Zohreh , Azima Sara , Motamedifar Mohammad , Kaviani Maasumeh , Poordast Tahereh , Zare Najaf 
Published: April 2020

<b><i>Background and Objective:</i></b> Healthy vaginal ecosystem is conserved with the connection of vaginal epithelium and flora in which lactobacilli play a significant role. The present study aimed to examine the effect of lactofem oral probiotic capsule on <i>Lactobacillus</i> colonization and some other vaginal health indices in women aged 18–55 years. <b><i>Materials and Methods:</i></b> This interventional, double-blind controlled clinical trial was conducted on 70 women with Nugent score = 4–6 and vaginal pH &#x3e;4.5. The participants were divided into an oral probiotic group and a control group. The oral probiotic group was required to take one 500 mg lactofem capsule daily for 2 months, while placebo was prescribed for the control group. Samples from 6 visits were examined during the period of prescription. The data were analyzed using the SPSS statistical software, version 18. <b><i>Results:</i></b> The results showed no significant difference in the average colonization of vaginal <i>Lactobacillus</i> in the 2 groups before and during the intervention (<i>p</i> = 0.26). Also, no significant difference was observed in the 2 groups’ Nugent mean scores before and after the intervention up to the 60th day. However, a statistically significant difference was found in this regard on the 70th day (<i>p</i> = 0.032). Moreover, the results indicated no significant difference in the 2 groups’ mean vaginal pH before and after the intervention (<i>p</i> = 0.101). <b><i>Conclusion:</i></b> Lactofem oral capsule could improve the participants’ Nugent scores, but caused no change in <i>Lactobacillus</i> colonization or vaginal pH.
Functional constipation masked as irritable bowel syndrome
Authors: Tosto Monica , D’Andrea Paola , Salamone Ignazio , Pellegrino Salvatore , Costa Stefano , Lucanto Maria Cristina , Pallio Socrate , Magazzu’ Giuseppe , Guandalini Stefano 
Published: April 2020

Abstract
Background
Rome IV criteria for functional gastrointestinal disorders state that children suspected of having Irritable Bowel Syndrome (IBS) with Constipation (IBS-C) should be preliminarily treated for constipation. We aimed at verifying if functional constipation may indeed lead to an erroneous diagnosis of IBS with diarrhea (IBS-D) or IBS with mixed pattern of diarrhea and constipation (IBS-M).

Methods
We prospectively enrolled in an unblinded fashion 10 and 16 consecutive children referred to our center who met Rome IV criteria for a diagnosis of IBS-D and IBS-M, respectively. Patients who fulfilled criteria for suspect “occult constipation” were then given a bowel cleaning regimen with Polyethylene glycol 3350, re-evaluated at 2 months and followed up for at least 6 months. Sixteen additional patients with IBS with Constipation (IBS-C) referred in the same period served as control. The endpoints were: 1) a decrease of more than 50% in abdominal pain intensity and frequency scores; and 2) for patients with IBS-D and IBS-M: resolution of diarrhea.

Results
The endpoints were met by 8 (80%) and 14 (87%) of the patients with IBS-D and IBS-M, respectively, with decrease of abdominal pain and resolution of “diarrhea”. The response was not significantly different from that observed in 15 (93%) of the IBS-C control group.

Conclusion
Acknowledging the limitations of the small number of patients and of the uncontrolled nature of the study, we suggest that a possibly large number of patients labeled as IBS-D or IBS-M may actually simply present functional constipation and should be managed as such.
lncRNA CRNDE promotes the proliferation and metastasis by acting as sponge miR-539-5p to regulate POU2F1 expression in HCC
Authors: Li Zhixi , Wu Gang , Li Jie , Wang Youyu , Ju Xueming , Jiang Wenjun 
Published: April 2020

Abstract
Background
This article focuses on the roles and mechanism of lncRNA CRNDE on the progression of HCC.

Methods
We used qRT-PCR to detect the expression of lncRNA CRNDE in HCC cells, normal cells and clinical tissues. MTT assay, FCM analysis, Transwell migration and invasion assay were used to detect the effects of lncRNA CRNDE on cell viability, apoptosis, migration and invasion of HCC cells. The expression of apoptosis-related proteins Bcl-2, Bax, Cleaved Caspase 3, Cleaved Caspase 9, EMT epithelial marker E-cadherin and mesothelial marker Vimentin were analyzed by Western blot. Online prediction software was used to predict the binding sites between lncRNA CRNDE and miR-539-5p, or miR-539-5p and POU2F1 3’UTR. Dual luciferase reporter assay, qRT-PCR and RNA pulldown were used to detect target-relationship between lncRNA CRNDE and miR-539-5p. Dual luciferase reporter assay, qRT-PCR, Western blot and Immunofluorescence were used to detect target-relationship between miR-539-5p and POU2F1. qRT-PCR was used to detect the expression of miR-539-5p and POU2F1 in clinical tissues. Rescue experiments was used to evaluate the association among lncRNA CRNDE, miR-539-5p and POU2F1. Finally, we used Western blot to detect the effects of lncRNA CRNDE, miR-539-5p and POU2F1 on NF-κB and AKT pathway.

Results
lncRNA CRNDE was highly expressed in HCC cells and HCC tissues compared with normal cells and the corresponding adjacent normal tissues. lncRNA CRNDE promoted the cell viability, migration and invasion of HCC cells, while inhibited the apoptosis and promoted the EMT process of HCC cells. lncRNA CRNDE adsorbed miR-539-5p acts as a competitive endogenous RNA to regulate POU2F1 expression indirectly. In HCC clinical tissues, miR-539-5p expression decreased and POU2F1 increased compared with the corresponding adjacent normal tissues. lncRNA CRNDE/miR-539-5p/POU2-F1 participated the NF-κB and AKT pathway in HCC.

Conclusion
lncRNA CRNDE promotes the expression of POU2F1 by adsorbing miR-539-5p, thus promoting the progression of HCC.
Scleral thinning surgery for bullous retinal detachment with retinal pigment epithelial tear in central serous chorioretinopathy: a case report
Authors: Maggio Emilia , Mete Maurizio , Maraone Giorgia , Arena Fabrizio , Pertile Grazia 
Published: April 2020

Abstract
Background
Bullous serous retinal detachment (RD) with retinal pigment epithelial (RPE) tear is a rare and severe variant of chronic central serous chorioretinopathy (CSC). Due to its atypical presentation, it may raise diagnostic issues, leading to inappropriate therapeutic procedures. The optimum treatment for this CSC variant is still uncertain.

Case presentation
A 65-year-old male was referred for vitreo-retinal surgery with a provisional diagnosis of rhegmatogenous RD in his right eye. Dilated fundus examination showed an inferior bullous RD with no evidence of retinal breaks, while a large RPE tear was detected in the temporal quadrant. Ocular ultrasound showed no mass lesion. The axial length was 23.63 mm. Enhanced depth imaging optical coherence tomography (EDI-OCT) revealed a pachychoroid pattern in both eyes. The patient referred a history of CSC in the right eye and the recent use of intravenous corticosteroids for bronchitis. Laser therapy and photodynamic therapy were not applicable due to the extension and elevation of the RD. Two months after oral treatment with eplerenone, the subretinal fluid increased significantly. The patient underwent two 4 × 4 mm deep lamellar sclerectomies in the inferior quadrants. The surgical treatment resulted in complete RD resolution.

Conclusion
A correct diagnosis of bullous variant of chronic CSC with RPE tear is critical to avoid inappropriate procedures and to prevent severe visual loss as a result of neuroretinal damage. Scleral thinning surgery may be considered a valid option, resulting in rapid and long-lasting resolution of RD.
Vaccination coverage rates and predictors of HPV vaccination among eligible and non-eligible female adolescents at the Brazilian HPV vaccination public program
Authors: Faisal-Cury Alexandre , Levy Renata Bertazzi , Tourinho Maria Fernanda , Grangeiro Alexandre , Eluf-Neto José 
Published: April 2020

Abstract
Background
Since March 2014, the quadrivalent HPV vaccine has been incorporated into the Brazilian Unified Health Care System and began to be offered, without direct costs, for girls from 9 to 13 years of age. Older female adolescents would have the option to be vaccinated at private health care system being responsible for the payment of HPV vaccine. The present study aimed to evaluate the coverage rates and predictors of HPV vaccination in Brazil among two groups of female adolescents: eligible and non-eligible for the HPV vaccination public program.

Methods
We used data from the 2015 Brazilian National Adolescent School-Based Health Survey, which involved a probabilistic sample of 5404 female adolescents students at public and private schools. Using a questionnaire, we gathered information on sociodemographic characteristics, sexual behavior, and respondent perception of parental supervision and have been vaccinated for HPV. Age-specific vaccination rates were analyzed in girls aged 9 to 13 at the time of public vaccination (eligible for public policy), as well among those 14 to 17 years old not eligible by the Ministry of Health for vaccination. We used Poisson regression models to investigate associated factors.

Results
HPV vaccine coverage was 83.5 and 21.8% among eligible and non-eligible populations, respectively. In both populations, the chance of being vaccinated decreased with older age. In the eligible population there is a greater chance of being vaccinated among ethnic group “pardas” but not with other indicators of socioeconomic status. In the non-eligible population, there was a clear association between higher vaccine coverage and greater maternal education and living with the mother.

Conclusion
Our findings highlight the importance of public policies to minimize inequities in access to cancer prevention measures in vulnerable adolescents. A public policy of HPV vaccination for older female adolescents would increase coverage with possible reduction of HPV-related diseases in this group of women.
Words matter: the prevalence of chiropractic-specific terminology on Australian chiropractors’ websites
Authors: Young Kenneth J. 
Published: April 2020

Abstract
Background
Chiropractors use words and phrases in unique ways to express traditional, chiropractic-specific theories. This lexicon represents concepts that reinforce the separation of chiropractic from other health care professions. It may impact referrals both to and from chiropractors, lead to public confusion about health care issues, and reduce cross-disciplinary research. Therefore, it is important to understand how prevalent chiropractic-specific terms are in publicly available media.

Methods
Five chiropractic terms were selected: subluxation, adjustment, vital (−ism/−istic), wellness, and Innate (Intelligence). States and territories in Australia were proportionately sampled according to population of chiropractors using a Google search for chiropractors’ private practice websites. The top results were recorded. Websites were word-searched on every publicly available page for the five terms. Context was checked to count only terms that were used to support a chiropractic-specific concepts. The number of occurrences of each term was recorded, tallied nationally and by state/territory. Descriptive statistics were applied to determine prevalence.

Results
Three hundred sixty-nine websites were sampled, based on an estimate of 5500 chiropractors practising in Australia. Nationally, 85% of chiropractors used one or more terms. The term adjust (−ing/−ment) occurred most frequently, being found on 283 websites (77%) with a total of 2249 occurrences. Wellness was found on 199 websites (54%) with 872 occurrences; subluxation was found on 104 websites (28%), 489 occurrences; vital (−ism/−istic) on 71 websites (19%) with 158 occurrences; and Innate was least used, being found on 39 websites (11%) with 137 occurrences.

Conclusion
A majority of the Australian chiropractors sampled used one or more chiropractic-specific terms on their websites. Future research should explore the effects of chiropractic language on the public, policy-makers, and other health care professionals.
mRNAs biomarker related to the control of proliferation and cell death in HepG2/C3A spheroid and monolayer cultures treated with piperlongumine
Authors: Biazi Bruna Isabela , Zanetti Thalita Alves , Marques Lilian Areal , Baranoski Adrivanio , Coatti Giuliana Castello , Mantovani Mário Sérgio 
Published: April 2020

Abstract
Background
Cell culture (spheroid and 2D monolayer cultures) is an essential tool in drug discovery. Piperlongumine (PLN), a naturally occurring alkaloid present in the long pepper (Piper longum), has been implicated in the regulation of GSTP1 activity. In vitro treatment of cancer cells with PLN increases ROS (reactive oxygen species) levels and induces cell death, but its molecular mode of action has not been entirely elucidated.

Methods
In this study, we correlated the antiproliferative effects (2D and 3D cultures) of PLN (CAS 20069–09-4, Sigma-Aldrich) with morphological and molecular analyses in HepG2/C3A cell line. We performed assays for cytotoxicity (MTT), comet assays for genotoxicity, induction of apoptosis, analysis of the cell cycle phase, and analysis of the membrane integrity by flow cytometry. Relative expression of mRNA of genes related to proliferation, apoptosis, cell cycle control, metabolism of xenobiotics, and reticulum endoplasmic stress.

Results
PLN reduced the cell proliferation by the cell cycle arrest in G2/M. Changes in the mRNA expression for CDKN1A (4.9x) and CCNA2 (0.5x) of cell cycle control genes were observed. Cell death occurred due to apoptosis, which may have been induced by increased expression of proapoptotic mRNAs (BAK1, 3.1x; BBC3, 2.4x), and by an increase in 9 and 3/7 active caspases. PLN induced cellular injury by ROS generation and DNA damage. DNA damage induced MDM2 signaling (3.0x) associated with the appearance of the monastral spindle in mitosis. Genes associated with ROS degradation also showed increased mRNA expression (GSR, 2.0x; SOD1, 2.1x). PLN induce endoplasmic reticulum stress with the increase in the mRNA expression of ERN1 (4.5x) and HSPA14 (2.2x). The xenobiotic metabolism showed increased mRNA expression for CYP1A2 (2.2x) and CYP3A4 (3.4x). In addition to 2D culture, PLN treatment also inhibited the growth of 3D culture (spheroids).

Conclusion
Thus, the findings of our study show that several gene expression biomarkers (mRNAs) and monastral spindle formation indicated the many pathways of damage induced by PLN treatment that contributes to its antiproliferative effects.

Graphical abstract
Gene expression of renal lactoferrin and glycemic homeostasis in diabetic rats with reference to the protective role of exogenous bovine lactoferrin
Authors: Qari Sameer H. , Attia Kamal 
Published: April 2020

Abstract
Background
This study is intended to clarify the influence of exogenous bovine lactoferrin (bLf) treatment on glycemic homeostasis, gene expression, and production of lactoferrin (Lf) in rats with experimentally induced diabetes.

Methodology
Fifty adult male rats were used; 15 rats were used as the normal control rats (group A) and were injected an intraperitoneal (I/P) with 1 ml of isotonic saline daily for 3 months. Thirty-five rats were administered I/P injection of 60 mg of streptozotocin per kilogram body weight to induce diabetes. Thirty streptozotocin-treated rats were selected and used as diabetic rats, and they were subdivided into 2 equal groups (15 rats each): the untreated diabetic group (group B) and treated diabetic group (group C). The rats in the diabetic group were administered an I/P injection of 1 ml of isotonic saline daily for 3 months, while rats in the diabetic treatment group were administered an I/P injection of 300 mg of bLf/kg body weight/day/3 months. At the end of the experiment, blood and renal tissue samples were collected from all rats. The levels of insulin, glucose, and lactoferrin in plasma were measured. Homeostatic model assessment for insulin resistance (HOMA-IR) was performed. The numbers of Lf-mRNA copies in renal tissue were assessed using quantitative RT-PCR. To measure Lf production in renal tissue, Western blot analysis was applied.

Results
The obtained data demonstrated that the treatment of diabetic rats with bLf maintained glycemic homeostasis at normal levels but increased the mRNA expression of renal Lf.

Conclusion
In diabetic rats, bovine lactoferrin treatment offers the potential for protection against incidences of insulin resistance (IR) by stimulating the suppressed expression and production of the LTF gene.
Evaluación ambiental de la producción a larga escala de microperlas de quitosano modificadas con tiourea como alternativa de valorización de residuos en el sector camaronicultor
Authors: Meramo-Hurtado Samir I. , Moreno-Sader Kariana , González Delgado Angel Darío 
Published: April 2020

La generación de residuos durante el procesamiento del camarón es un alarmante problema de salud pública y medioambiental, lo que ha motivado a la búsqueda de alternativas de valorización de estos residuos tal como la producción de quitosano. Debido a sus múltiples propiedades, este biopolímero ha sido utilizado en diferentes sectores como el farmacéutico, tratamiento de aguas y biomedicina. En esta investigación, se realizó la simulación y evaluación ambiental de un proceso químico enfocado en la conversión de quitosano a partir del exoesqueleto de camarón hacia microperlas de quitosano modificadas con tiourea. Para ello, se utilizó el software de simulación Aspen Plus® y el algoritmo de reducción de residuos (WAR). Cuatro casos fueron considerados referentes a las contribuciones de energía y productos. El rendimiento de producción de las microperlas (MPs) fue de 2 kg MPs-Tiourea/kg quitosano. La evaluación ambiental mostró que el caso 3 alcanzó el valor más alto para impactos totales de salida (1.23×10+3 PEI/h), seguido por el caso 2, caso 1 y caso base. Se concluyó que la contribución de energía es menor a la del flujo de productos y que el proceso es amigable con el medio ambiente para las categorías de impactos atmosféricos y toxicológicos.
Criminología & complejidad humana
Authors: Venera Redondo Ronald Enrique 
Published: April 2020

El presente artículo de refl exión registra cómo históricamente el ser humano ha tratado de explicar usando disímiles medios provenientes de las diferentes ciencias humanas y naturales, el origen y causa del delito en la sociedad, diversifi cando las múltiples explicaciones sobre el móvil de las conductas delictivas que emanan del ser humano. Así las cosas, este trabajo tiene como fin tratar de explicar y entender el delito como un acto de evolución del ser humano, hacia un estado de necesidad, en el cual el delito se convierte en un instrumento irrenunciable del agresor con el objeto de satisfacer desviaciones que ha adquirido con el paso de su historia.
Falencias en la protección del derecho a la salud: una mirada a la población desplazada
Authors: Aristizábal Gómez Kelly Viviana 
Published: April 2020

El presente artículo de revisión tiene como fi nalidad identifi car las principales falencias en materia de protección al derecho a la salud de la población que se encuentra en situación de desplazamiento forzado, a partir de un análisis juicioso que inicia con una descripción del origen del fenómeno del desplazamiento en Colombia, continúa con la evolución normativa y jurisprudencial que ocupa al tema, precisa el alcance jurídico que se le atribuye al derecho a la salud, para fi nalmente desarrollar cada una los aspectos que más limitaciones y afectación al derecho a la salud han generado al pretender aplicar la regulación normativa existente al respecto a las personas que se encuentran en situación de desplazamiento forzado.
Los valores y la interpretación material, sistemática y evolutiva de la constitución y los derechos humanos a la luz del tridimensionalismo jurídico
Authors: Picado Vargas Carlos Adolfo 
Published: April 2020

Este es un artículo en el cual se expuso un análisis sobre la relevancia de la interpretación jurídica en el Derecho Constitucional y la protección de los derechos fundamentales y humanos, en aras de consolidar un Estado Social de Derecho, en el cual se persiga el carácter práctico de la ley, pero sin obviar quiénes serán sus destinatarios. Asimismo, exaltar la incidencia de los valores y principios en la interpretación, comprensión y aplicación de la ley por parte de los operadores judiciales, teniendo en cuenta las nuevas circunstancias fácticas y el contexto histórico cambiante, la necesidad de impartir juicios en los que se aplique la ponderación y razonabilidad, estableciendo niveles jerárquicos, remitiéndose a precedentes constitucionales; respetando ciertos parámetros, deberes propios del ordenamiento jurídico, con miras a regular las conductas de los ciudadanos en sociedad.
¿Es Colombia una nación diasporita y transnacional? Análisis del nuevo discurso del estado colombiano frente a la diáspora
Authors: Mej Mar Fernanda 
Published: April 2020

Con motivo del aumento exponencial en el número de nacionales que han abandonado el país en la última década y media, el Estado colombiano ha incorporado un nuevo discurso y diseñado nuevos derechos específicos para la diáspora. Es así como hoy Colombia se define oficialmente como una Nación diaspórica y transnacional, generando con ello un cambio sin precedentes en la forma cómo concibe la institución y el ejercicio ciudadanos. A la luz de las propuestas del modelo transnacional de ciudadanía, el presente artículo analiza el alcance de estos nuevos derechos en el contexto migratorio colombiano, intentando dar luces sobre cuán cerca se encuentra Colombia de un nuevo estatus transnacional de ciudadanía. Sostenemos que, a pesar de representar transformaciones importantes en la concepción y ejercicio ciudadanos, la consolidación de un estatus transnacional de ciudadanía se encuentra aún lejos de materializarse.

AbstractDue to the large numbers of Colombians who have left their country in the last decade and a half, Colombia has adopted a new discourse as well as a new set of rights specifically designed for the diaspora. Hence, Colombia now defines itself as diasporic and transnational nation, bringing forward unprecedented changes on how the institution and exercise of citizenship is conceived. In light of the proposals coming from the transnational citizenship model, this article examines and analyzes the scope to which this new set of rights brings Colombia closer or not to a new transnational citizenship status. We argue that, despite generating important transformations on the idea and exercise of citizenship, the consolidation of a transnational citizenship status is still far from materializing.