bluuebunny/crossref_metadata_2025_split huggingface dataset:
Single nucleotide polymorphism analysis in breast cancer using MassARRAY from Jammu and Kashmir, India
Authors: Bakshi Divya , Nagpal Ashna , Sharma Varun , Sharma Indu , Shah Ruchi , Sharma Bhanu , Bhat Amrita , Verma Sonali , Bhat Gh. Rasool , Abrol Deepak , Sharma Rahul , Vashnavi Samantha , Kumar Rakesh 
Published: July 2020

Abstract
BackgroundBreast Cancer (BC) is associated with inherited gene mutations. High throughput genotyping of BC samples has led to the identification and characterization of biomarkers for diagnosis of BC. The most common genetic variants studied are SNPs (Single Nucleotide Polymorphisms) that determine susceptibility to an array of diseases thus serving as a potential tool for identifying the underlying causes of breast carcinogenesis. MethodsSNP genotyping employing the Agena MassARRAY offers a robust, sensitive, cost-effective method to assess multiple SNPs and samples simultaneously. In this present study, we analyzed 15 SNPs of 14 genes in 550 samples (150 cases and 400 controls). We identified four SNPs of genes TCF21, SLC19A1, DCC, and ERCC1 showing significant association with BC in the population under study. ResultsThe SNPs were rs12190287 (TCF21) having OR 1.713 (1.08-2.716 at 95% CI) p-value 0.022 (dominant), rs1051266 (SLC19A1) having OR 3.461 (2.136-5.609 at 95% CI) p-value 0.000000466 (dominant), rs2229080 (DCC) having OR 0.6867 (0.5123 -0.9205 at 95% CI) p-value 0.0116 (allelic) and rs2298881 (ERCC1) having OR 0.669 (0.46-0.973 at 95% CI), p-value 0.035 (additive) respectively. The in-silico analysis was further used to fortify the above findings. ConclusionIt is further anticipated that the variants should be evaluated in other population groups that may aid in understanding the genetic complexity and bridge the missing heritability.
Mutational Profiles of Marker Genes of Cervical Carcinoma in Bangladeshi patients
Authors: Sharmin Shahana , Zohura Fatima Tuj , Islam Md. Sajedul , Shimonty Anika , Khan Md. Abdullah-Al-Kamran , Perveen Rehana , Sharmin Foujia , Ahsan Chowdhury Rafiqul , Islam Abul BMMK , Yasmin Mahmuda 
Published: July 2020

Abstract
Background: Cervical cancer is a gynecologic cancer type that develops in the cervix, accounting for 8% mortality of all female cancer patients. Infection with specific human papillomavirus (HPV) types is considered the most severe risk factor for cervical cancer. In the context of our socioeconomic conditions, an increasing burden of this disease and high mortality rate prevail in Bangladesh. Although several researches related to the epidemiology, HPV vaccination, and treatment modalities were conducted, researches on the mutation profiles of marker genes in cervical cancer in Bangladesh remain unexplored. Methods: In this study, five different genomic regions within the top three most frequently mutated genes (EGFR, KRAS and PIK3CA) in COSMIC database with a key role in the development of cervical cancers were selected to study the mutation frequency in Bangladeshi patients. In silico analysis was done in two steps: nucleotide sequence analysis and its corresponding amino acid analysis.Results: DNA from 46 cervical cancer tissue samples were extracted and amplified by PCR, using 1 set of primers designed for EGFR and 2 sets of primers designed for two different regions of both PIK3CA and KRAS gene. In total, 39 mutations were found in 28 patient samples. Eleven different mutations (23.91%), twenty-four different mutations (52.17%) and four mutations (8.7%) were found in amplified EGFR, PIK3CA and KRAS gene fragments, among which 1 (EGFR) was common in seven patient samples and 2 (PIKCA) were found in more than 1 patient. Our study shows that except for KRAS, the frequency of observed mutations in our patients is higher than those reported earlier in other parts of the world. Most of the exonic mutations were found only in the PIK3CA and EGFR genes.Conclusions: The study can be used as a basis to build a mutation database for cervical cancer in Bangladesh with the possibility of targetable oncogenic mutations. Further exploration needs to establish future diagnostics, personalized medicine decisions, and other pharmaceutical applications for specific cancer subtypes.
P2X7 Receptor Antagonist Attenuates Retinal Inflammation and Neovascularization Induced by Oxidized Low Density Lipoprotein
Authors: Yang Mingzhu , Qiu Ruiqi , Wang Weiping , Liu Jingyang , Li Ya , Zhu Qing , Lei Bo 
Published: July 2020

Abstract
Background: Inflammation and neovascularization are two vital pathological phases of AMD. Recent evidence indicates that blocking P2X7 receptor may relieve inflammation. Here, we investigated whether A740003, a P2X7 receptor antagonist, could prevent retinal inflammation and neovascularization induced by ox-LDL and explored the underlying mechanisms.Methods: ARPE-19 cells were pretreated with A740003 for 2 hours before ox-LDL exposure. Western Blot, ELISA and qPCR were used to detect the proteins and mRNA levels of NLRP3, Caspase-1, P2X7R, pIKBa, IKBa, angiogenic factors and inflammatory cytokines. ROS assay was performed to detect the ROS generation. C57BL/6 mice were subretinally injected with ox-LDL to induce retinal inflammation and neovascularization. A740003 was administrated intraperitoneally starting from day 3 before to day 14 after ox-LDL injection. Retinal function was assessed by dark- and light-adapted ERG. Retinal neovascularization was detected by retinal whole flat mount and immunofluorescence. Thickness of INL and ONL was measured to evaluate the retinal edema. Results: ox-LDL induced inflammatory responses and VEGF overproduction in human RPE cells and mouse retinas. However, A740003 decreased the expression of NLRP3, P2X7R, HIF-1a and VEGF in ARPE-19 cells. Inflammatory cytokines including IL-1b and IL-18 were down-regulated in A740003 treated cells. A740003 suppressed ROS generation and inhibited the phosphorylation of IKBa significantly. Whole flat mount of retinas and immunofluorescence showed that A740003 inhibited the angiogenesis induced by ox-LDL in vivo. The elevated mRNA and protein levels of NLRP3, Caspase-1, P2X7R, HIF-1a and VEGF decreased and IL-1b release was repressed in A740003 treated mice. Besides, the phosphorylation of IKBa was suppressed in the retinas of A740003 treated mice. The retinal edema was alleviated by A740003 treatment. The b- and a-wave amplitudes of ERG were remarkably preserved in A740003 treated mice. Conclusions: A740003 significantly reduced ocular inflammatory responses and VEGF production in ARPE-19 cells. In addition, the P2X7R antagonist reduced retinal inflammation, neovascularization, and protected retinal function in mice. The protective effects were associated with regulating of NLRP3 inflammasome and NF-kB pathway, suppression of ROS generation, as well as inhibition of angiogenic factors.
The Impact of Leadership Autonomy Support, Psychological Capital and Creativity on Hospital Employees’ Innovative Behaviour
Authors: Slåtten Terje , Lien Gudbrand , Mutonyi Barbara Rebecca 
Published: July 2020

Abstract
Background: There is growing interest in and focus on healthcare services research to identify factors associated with innovation in healthcare organizations. However, previous innovation research has concentrated primarily on the organizational level. In contrast, this study focuses on innovation by individual employees. The specific aim is to examine factors with potential impact on individual employee innovation in hospital organizations. Thus, the study significantly deepens and broadens previous research on innovation in the domain of health services.Methods: A conceptual model was developed and tested on a sample of hospital employees. Partial least-squares structural equation modelling (PLS-SEM) was used to analyse the data with Stata software. Mediation analysis was used to test the proposed indirect effects.Results: Hospital employees’ individual innovative behaviour is directly and positively associated with individual creativity (b = 0.440), psychological capital (b = 0.34) and leadership autonomy support (b = 0.07). The relationships between leadership autonomy support, psychological capital and individual innovative behaviour are all mediated by employees’ creativity. Psychological capital mediates the relationship between leadership autonomy support and individual innovative behaviour. Overall, the proposed model explains 50% of the variance in hospital employees’ innovative behaviour.Conclusions: This study reveals a complex pattern of links between innovative behaviour and leadership autonomy support, employees’ creativity and employees’ psychological capital. However, the findings indicate that leadership autonomy support has a dominant and multifaceted impact on hospital employees’ innovative behaviour.
Natural Course of Fat Necrosis After Breast Reconstruction: A 10-Year Follow-Up Study
Authors: Lee Jeeyeon , Park Ho Yong , Kim Wan Wook , Lee Jeong Ju , Keum Hee Jung , Yang Jung Dug , Lee Jeong Woo , Lee Joon Seok , Jung Jin Hyang 
Published: July 2020

Abstract
Background: Although fat necrosis is a minor postoperative complication after breast reconstruction, occasionally it mimics to tumor recurrence in patients with breast cancer. Therefore, the surgeon should distinguish between benign fat necrosis and true local recurrence. The authors evaluated the clinical characteristics of fat necrosis after breast reconstruction and investigated the natural course of fat necrosis.Methods: Between 2007 and 2013, a total of 362 patients underwent breast reconstruction after partial or total mastectomy for breast cancer in Kyungpook National University Hospital. Clinicopathologic characteristics and the occurrence of fat necrosis were assessed during surveillance for 10 years of mean follow-up period.Results: There were 42 cases (11.6 %) of fat necrosis after breast reconstruction with partial or total mastectomy which were confirmed by needle or excision biopsy. The fat necrosis was resolved after a mean period of 45.9 months (SD, ± 42.1) and 26 cases (61.9 %) of fat necrosis were almost completely resolved (less than 5 mm) during 10-year follow-up period.Conclusion: Based on the natural course of fat necrosis, the fat necrosis after breast reconstruction can be only monitored, if pathologic confirmation was done. More than half of the cases will be resolved within 2–3years.
LncRNA LEF1-AS1 Promotes Metastasis of Prostatic Carcinoma via the Wnt/β-Catenin Pathway
Authors: Li Weiyuan , Yang Ganggang , Yang Dengke , Li Dong , Sun Qian 
Published: July 2020

Abstract
Background: Long noncoding RNAs (lncRNAs), which are important functional regulators in cancer, have emerged as critical molecular regulators in various biological processes. However, the mechanisms by which LEF1-AS1 modulates Androgen-Independent Prostate Cancer (AIPC) development remain largely unknown.Methods: The LEF1-AS1 expression level was detected in tumour tissues and adjacent normal tissues of AIPC patients by using next-generation sequencing technology and qRT-PCR. Cell proliferation, migration and invasion were assessed by colony formation, EDU assays and transwell assays, respectively. Xenograft assay was conducted to determine the effect of LEF1-AS1 on cell proliferation in vivo.Results: LEF1-AS1 promoted the proliferation, migration, invasion and angiogenic ability of AIPC cells in vitro and in vivo. In this mechanism, LEF1-AS1 recruited the transcription factor C-myb to the promoter region of FZD2, which activated FZD2 transcription. Moreover, LEF1-AS1 functioned as a competing endogenous RNA (ceRNA) acting as a sponge for miR-328, which activated CD44.Conclusion: Collectively, these data indicate that LEF1-AS1 is a tumour promoter in the development of AIPC and that it may contribute to the improvement of AIPC diagnosis and therapy.
Primary Bone Lymphoma of the Humerus (About A Case and Literature Review)
Authors: Yahyaoui Mounir , Aharram Soufiane , Amghar Jawad , Agoumi Omar , Daoudi Abdelkrim 
Published: July 2020

Abstract
Background: Primary localised bone lymphoma is a rare affection prevalent in humans. It often involves long bones, but can be found in all the skeleton bones. The diagnosis is based on the anatomopathological study with immunohistochemical study of a bone biopsy. Case presentation: We also report the first diagnosed case of primary bone lymphoma of the humerus since the inauguration of our university hospital (since September 2014).Conclusions: It is an extremely rare pathology, of which the orthopedist must know. Treatment is based on chemotherapy (with or without anti-CD20) followed by radiotherapy. The prognosis of primary localised bone lymphoma is very favorable.
A phase II randomized trial of sodium oligomannate in Alzheimer's dementia
Authors: Wang Tao , Kuang Weihong , Chen Wei , Zhang Liming , Xu Wenwei , Li Yingjie , Li Hailin , Peng Ying , Chen Yangmei , Wang Baojun , Xiao Jinsong , Li Honghua , Yan Chuanzhu , Du Yifeng , Tang Mouni , He Zhiyi , Chen Haibo , Li Wei , Lin Hong , Shi Shugui , Bi Jianzhong , Zhou Huadong , Cheng Yan , Gao Xiaoping , Guan Yihui , Huang Qiu , Chen Kewei , Xin Xianliang , Ding Jian , Geng Meiyu , Xiao Shifu 
Published: July 2020

Abstract
Background: Sodium oligomannate (GV-971), a marine-derived oligosaccharide, is a novel agent that may improve cognition in AD patients.Methods: The 24-week multi-center, randomized, double-blind, placebo parallel controlled clinical trial was conducted in AD in China between 24 October 2011 and 10 July 2013. The study included a 4-week screening/washout period, followed by a 24-week treatment period. Patients were randomized 1:1:1 ratio to receive GV-971 900 mg, 600 mg, or placebo capsule in treatment period, respectively. The primary outcome was cognitive improvement as assessed by changes in Alzheimer's Disease Assessment Scale-cognitive subscale 12-item (ADAS-cog12) scores from baseline to week 24. The secondary efficacy outcomes included CIBIC-Plus, ADCS-ADL and NPI at 24 weeks after treatment compared with baseline. A sub-group study was assessment of the change in cerebral glucose metabolism by fluorodeoxyglucose positron emission tomography measurements.Results: Comparing with placebo group (n=83, change -1.45), the ADAS-Cog12 score change in GV-971 600mg group (n=76) was -1.39 (p=0.89), GV-971 900mg group (n=83) was -2.58 (p=0.30). The treatment responders according to CIBIC-plus assessment was significantly higher in GV-971 900mg group than placebo group (92.77% vs 79.52%, p<0.05). GV-971 900mg subgroup showed a significantly lower cerebral metabolic rate for glucose decline than the placebo subgroup at the left precuneus, right posterior cingulate, and right hippocampus. The respective rates of treatment-related AEs were 5.9%, 14.3%, and 3.5%.Conclusions: GV-971 was safe and well tolerated. GV-971 900mg was chosen for phase III clinical study.Trial registration: ClinicalTrials.gov, NCT01453569. Registered 18 October, 2011, https://clinicaltrials.gov/ct2/show/NCT01453569?term=NCT01453569&draw=2&rank=1.
Mental health impacts among health workers during COVID-19 in a low resource setting: a cross-sectional survey from Nepal
Authors: Khanal Pratik , Devkota Navin , Dahal Minakshi , Paudel Kiran , Joshi Devavrat 
Published: July 2020

Abstract
Background: Health care workers exposed to COVID-19 might be at risk of developing mental health problems. The study aimed to identify factors associated with anxiety, depression and insomnia among health workers involved in COVID-19 response in Nepal. Methods: This was a cross-sectional web-based survey conducted in between April 26 to May 12, 2020. A total of 475 health workers participated in the study. Anxiety and depression were measured using 14-item Hospital Anxiety and Depression Scale (HADS: 0- 21) and insomnia was measured by using 7-item Insomnia Severity Index (ISI: 0-28). Multivariable logistic regression analysis was done to determine the risk factors of mental health outcomes. Results: Overall, 41.9% of health workers had symptoms of anxiety, 37.5% had depression symptoms and 33.9% had symptoms of insomnia. Stigma faced by health workers was significantly associated with higher odds of experiencing symptoms of anxiety (AOR: 2.47; 95% CI: 1.62-3.76), depression (AOR: 2.05; 95% CI: 1.34-3.11) and insomnia (AOR: 2.37; 95% CI: 1.46-3.84). History of medication for mental health problems was significantly associated with higher likelihood of experiencing symptoms of anxiety (AOR: 3.40; 95% CI:1.31-8.81) , depression (AOR: 3.83; 95% CI: 1.45-10.14) and insomnia (AOR: 3.82; 95% CI: 1.52-9.62) while inadequate precautionary measures in the workplace was significantly associated with higher odds of exhibiting symptoms of anxiety (AOR: 1.89; 95% CI: 1.12-3.19) and depression (AOR: 1.97; 95% CI: 1.16-3.37). Nurses (AOR: 2.33; 95% CI: 1.21-4.47) were significantly more likely to experience anxiety symptoms than other health workers. Conclusion: The study findings revealed higher proportion of anxiety, depression and insomnia among health workers during the early phase of the pandemic in Nepal. Health workers facing stigma, those with history of medication for mental health problem, and those reporting to having inadequate precautionary measures in their workplace were more at risk of developing mental health outcomes. A focus on improving mental health wellbeing of health workers should be immediately initiated with attention to reduction of stigma, ensuring adequate support system such as personal protective equipments, and family support for those with history of mental health problems.
CANCER OF THE FEMALE REPRODUCTIVE SYSTEM IN THE FAR EASTERN FEDERAL DISTRICT
Authors: Gordienko V. P. , Leontjeva S. N. , Korobkova T. N. 
Published: July 2020

The aim of the study was to assess the incidence and mortality rates of female reproductive system cancer in the Far Eastern Federal District over the last ten years (2008–2017).Material and Methods. In our study we used statistical reports on the female reproductive system cancer incidence and mortality rates and 10-year follow-up data.Results. In 2017, 25155 new cases of female reproductive system cancer were diagnosed in the Far Eastern Federal District (26.6 % higher than in 2008). A total of 128776 patients (2.1 % of the population of the region) were followed up. Over the study period, there has been increase in the number of patients with stage I–II breast cancer (+4.8 %), cervical cancer (+6.3 %), uterine body (+3.9 %) and ovarian cancer (+5.7 %), with simultaneous reduction in the number of patients with advanced stages, except ovarian cancer (+5.0 %). For the past 10 years, cancer incidence rates have been steadily increasing, while cancer mortality rates have been decreasing with the exception of cervical cancer (+13.7 %). Morphological confirmation of the diagnosis (over 90 %) exceeded the national average ones. The number of patients followed-up for 5 or more years appeared to be lower than the average values in Russia; however the 1-year mortality rates after diagnosis improved. Ovarian cancer remains the most deadly of the gynecological cancers (22.9–27.9 %).Conclusion. Over the last ten years in the Far Eastern Federal District, in contrast to the overall decline in the mortality rates, there has been a steady increase in the incidence rates of female reproductive system cancer, except for cervical cancer.
Death in Krčmar: A Contribution to the Quantification of the Victims of the 1918 Spanish Flu Pandemic
Authors: Krivošejev Vladimir 
Published: July 2020

It is estimated that the Spanish flu pandemic, which affected the entire planet from 1918 to 1919, affected about five hundred million people, or one-third of the world's population at the time, and killed about fifty million people. The disease was noticed among Serbian soldiers in Corfu in April 1918, and in May among soldiers on the Salonika Front, but without fatal consequences. During the summer, fatalities were also reported, mainly due to lung compaction. Then the epidemic was reported in occupied Serbia as well. Just at the time of the breakthrough of Salonika Front on the 15th of September, a new wave of the epidemic started, this time fatal. Many soldiers remained lying and dying in military hospitals set up along the way. Some soldiers made it home but then passed away, and some found their homes empty. In occupied Serbia, mass dying began before liberation. In the region of Valjevo, the first deaths occurred in early October, but mass deaths started in late October. This lasted less than two months. Then, by the beginning of the spring of 1919, sporadic deaths due to "pneumonia" were seen as a common complication of Spanish flu, which does not necessarily mean that the epidemic had stopped, but that its end did not have any fatal consequences. In the lowlands of the Valjevo region, mortality was relatively low (in the parish of the church in Rabrovica - 0,44%), in the hilly area the mortality rate was slightly higher (in the parish of the church in Brankovina - 1,32%), and in the high mountain areas, it was very high. An analysis of the number of recorded deaths in the books of the church in Krčmar indicates that over these two months 141 people (4.47% of the population) died in seven villages that belong to the parish of this church. That number is almost equal to the number of deaths from all possible causes over a 33month period: throughout 1917, the first 9 months of 1918, and throughout 1919. The highest mortality rate , 9,38% of the population, was recorded in the village of Mratišić, and the lowest, 1,61% in the village of Gornji Lajkovac. A higher number of deaths were recorded among the female population, but this can be attributed to the decrease in the number of males due to previous years of war.
Ancient Myth in Brazilian Cinematography: Black Orpheus
Authors: Stevanović Lada 
Published: July 2020

The paper deals with two film adaptations of the myth of Orpheus that were made in Brazil in 1959 and 1999. In view of the fact that in both films Orpheus appears as Afro-Brazilian, these two versions of the myth may be related to Sartre’s concept of Black Orpheus, and the movement of Negritude that appeared in Paris in the 1930s as an answer of the black francophone intellectuals to racial myths and colonial stereotypes. Regarding the fact that the European attitude towards ancient Greece is also characteristic of another kind of colonialism that is cultural, based on the claim of exclusive right to this past, the ancient myth that often appears only as a mirror of this relationship, functions in these films as a space for subversion and resistance to different types of colonial power. The films that are the focus of this paper are Black Orpheus (1959) directed by Marcel Camus, which is a French-Italian-Brazilian co-production, and Orpheus (1999) by Carlos Diegues, an entirely Brazilian production. Both films were inspired by the theatrical play written by Vinícius de Moraes, Orfeu da Conceição (1953). Although he himself participated in the production of Camus’s Black Orpheus, in the end he refused to be credited because in the film, the main idea of his drama was lost, which was to show Afro-Brazilians as the main protagonists of the Greek myth pointing out injustices and difficulties of Afro-Brazilian people in social reality, but also in the context of cultural and racial hegemony that clearly claimed the ancient Greek myth to be the heritage of European white men. Similarly, disappointed, Carlos Diegues decided to make another film together with Vinícius de Moraes. However, the plan was interrupted by Moraes death in 1980, but Diegues succeeded in filming it in 1999. The paper compares the two films focusing on the question to which extent these films challenge or confirm European cultural elitism, racial stereotypes and class inequalities.