bluuebunny/crossref_metadata_2025_split huggingface dataset:
A spontaneous genetically-induced epiallele at a retrotransposon shapes host genome function
Authors: Bertozzi Tessa M. , Hanin Geula , Takahashi Nozomi , Kazachenka Anastasiya , Ferguson-Smith Anne C. 
Published: November 2020

AbstractIntracisternal A-particles (IAPs) are endogenous retroviruses (ERVs) responsible for most insertional mutations in the mouse. Full-length IAPs harbour genes flanked by long terminal repeats (LTRs). Here, we identify a solo LTR IAP variant (C57iap1solo) recently formed in the inbred C57BL/6J mouse strain. In contrast to the C57BL/6J full-length IAP at this locus (C57iap1full), C57iap1solo lacks DNA methylation and H3K9 trimethylation. The distinct DNA methylation levels between the two alleles are established during preimplantation development, likely due to loss of KRAB zinc finger protein binding at the C57iap1solo variant. C57iap1solo methylation increases and becomes more variable in a hybrid genetic background yet is unresponsive to maternal dietary methyl supplementation. Differential epigenetic modification of the two variants is associated with metabolic differences and tissue-specific changes in adjacent gene expression. Our characterisation of C57iap1 as a genetically-induced epiallele with functional consequences establishes a new model to study transposable element repression and host-element co-evolution.
Phenotypic plasticity, population structure and adaptation in a young weed species with a worldwide distribution
Authors: Cornille A. , Tiret M. , Salcedo A. , Huang H.R. , Orsucci M. , Milesi P. , Kryvokhyzha D. , Holm K. , Ge X.J. , Stinchcombe J.R. , Glémin S. , Wright S.I. , Lascoux M 
Published: November 2020

SummaryThe colonization success of a species depends on phenotypic plasticity, adaptive potential and population structure. Assessing their relative contributions during a colonization process is challenging, and a large-scale experiment had yet to be done. In this study, we attempted to tease apart their effects on the fitness of one of the most common plant on Earth, the shepherd’s purse (Capsella bursa-pastoris), a self-fertilizing and allopolyploid weed, with a worldwide distribution. The overarching goal is to eventually understand how the shepherd’s purse extensive distribution range was established so rapidly. To do so, we carried out three common gardens, located in Europe, Asia and North America, and measured several life-history traits on field-collected accessions belonging to three distinct genetic clusters (Middle East, Europe, and Asia). Our experiment showed that (i) the success of C. bursa-pastoris is mainly due to its high degree of phenotypic plasticity; and (ii), genetic cluster effect reflected a classic pattern observed in core vs marginal populations, with the Middle Eastern cluster (putative core population) outperforming the European and Asian clusters. This study therefore revealed, in a model species, different relative contributions of plasticity and adaptation to fitness, depending on the population and the time since colonization occurred.
Kcnq2/Kv7.2 controls the threshold and bihemispheric symmetry of cortical spreading depolarization
Authors: Aiba Isamu , Noebels Jeffrey L 
Published: November 2020

AbstractSpreading depolarization (SD) is a slowly propagating wave of massive cellular depolarization associated with acute brain injury and migraine aura. Genetic studies link molecular defects in enhanced cytoplasmic Ca2+ flux, glial Na+-K+ ATPase, and interneuronal Na+ current with SD susceptibility, emphasizing the important roles of synaptic activity and extracellular ionic homeostasis in determining SD threshold. In contrast, gene mutations in ion channels that shape intrinsic membrane excitability are frequently associated with epilepsy susceptibility. It is not well known whether epileptogenic mutations in voltage-gated potassium channels that regulate membrane repolarization also modify SD threshold and generation pattern. Here we report that the Kcnq2/Kv7.2 potassium channel subunit, frequently mutated in developmental epilepsy, is an SD modulatory gene with significant control over seizure-SD transition threshold, bilateral cortical expression, and temporal susceptibility. Chronic DC-band cortical EEG recording from awake conditional Kcnq2 deletion mice (Emx2cre/+::Kcnq2flox/flox) revealed spontaneous cortical seizures and SD. In contrast to a related potassium channel deficient model, Kv1.1 KO mice, spontaneous cortical SDs in Kcnq2 cKO mice are tightly coupled to the terminal phase of seizures, arise bilaterally, and are observed predominantly during the dark phase. Administration of the nonselective Kv7.2 inhibitor XE991 to Kv1.1 KO mice reproduced the Kcnq2 cKO-like SD phenotype (tight seizure coupling and bilateral symmetry) in these mice, indicating that Kv7.2 currents directly and actively modulate SD properties. In vitro brain slice studies confirmed that Kcnq2/Kv7.2 depletion or pharmacological inhibition intrinsically lowers the cortical SD threshold, whereas pharmacological activators elevate the threshold to multiple depolarizing and hypometabolic SD triggers. Together these results identify Kcnq2/Kv7.2 as a distinctive SD regulatory gene, and point to SD as a potentially significant pathophysiological component of KCNQ2-linked epileptic encephalopathy syndromes. Our results also implicate KCNQ2/Kv7.2 channel activation as an adjunctive therapeutic target to inhibit SD incidence.
Quantifying the relevance of alternative stable states in many-species food webs
Authors: Karatayev Vadim A. , Baskett Marissa L. , van Nes Egbert H. 
Published: November 2020

AbstractAlternative stable ecosystem states are possible under the same environmental conditions in many models of 2-3 interacting species and an array of feedback loops. However, multi-species food webs might dissipate the feedbacks that create alternative stable states through species-specific traits and feedbacks. To test this potential, we develop a manyspecies model of consumer-resource interactions with two classes of feedbacks: specialized feedbacks where individual resources become unpalatable at high abundance, or aggregate feedbacks where overall resource abundance reduces consumer recruitment. We quantify how trophic interconnectedness and species differences in demography affect the potential for either feedback to produce alternative stable states dominated by consumers or resources. We find that alternative stable states are likely to happen in many-species food webs when aggregate feedbacks or lower species differences increase redundancy in species contributions to persistence of the consumer guild. Conversely, specialized palatability feedbacks with distinctive species roles in consumer guild persistence reduce the potential for alternative states but increase the likelihood that losing vulnerable consumers cascades into a food web collapse at low stress levels, a dynamic absent in few-species models. Altogether, among-species trait variation can limit the set of processes that create alternative stable states and impede consumer recovery from disturbance.
Automated monitoring of animal behaviour with barcodes and convolutional neural networks
Authors: Gernat Tim , Jagla Tobias , Jones Beryl M. , Middendorf Martin , Robinson Gene E. 
Published: November 2020

AbstractBarcode-based tracking of individuals revolutionizes the study of animal behaviour, but further progress hinges on whether specific behaviours can be monitored. We achieve this goal by combining information obtained from the barcodes with image analysis through convolutional neural networks. Applying this novel approach to a challenging test case, the honeybee hive, we reveal that food exchange among bees generates two distinct social networks with qualitatively different transmission capabilities.
Digital transformation of TSG from a B2B to a B2C company
Authors: Pingali Srinivas Rao , Shah Grishma 
Published: November 2020

Learning outcomes
The main objective of this marketing strategy case is to understand why and how a firm should evaluate and rework its own business to stay competitive and aligned with external market forces.

Case overview/synopsis
Technical Solutions Group (TSG), was the India-based technical support division of Quatrro Global Services and focused on providing support to customers of large Original Equipment Manufacturers (OEMs) and software developers around the globe. Because of the increased competition and structural changes at the customer end, the business was facing flattened revenue growth and a drop in margins. TSG had to make strategic decisions on how to continue the business, either within the current paradigm of being a business to business business or transforming into a business to consumer business leveraging digital technology.

Complexity academic level
This case can be used in an undergraduate (upper level) or graduate-level course on marketing strategy, digital strategy or strategic management.

Supplementary materials
Teaching Notes are available for educators only.

Subject code
CSS 11: Strategy.
Exploring hotel employees’ regulatory foci and voice behavior: the moderating role of leader-member exchange
Authors: Dai You-De , Zhuang Wen-Long , Yang Po-Kai , Wang Yi-Jun , Huan Tzung-Cheng 
Published: November 2020

PurposeDrawing on leader-member exchange theory and regulatory focus theory, the purpose of this study is to explore the effects of hotel employees’ regulatory foci on their voice behavior and the moderating role of leader-member exchange.Design/methodology/approachThe questionnaire includes demographics, regulatory foci, leader-member exchange and voice behavior sections. The data was collected via a survey of 10 international tourist hotels in Taiwan and 479 valid questionnaires were completed. Confirmatory factor analysis and path analysis were used to test the composite reliability, discriminant validity and convergent validity. Multiple regression analysis was used to test the hypotheses.FindingsThe outcome of this study indicates that both promotion focus and prevention focus can benefit employees’ voice behavior; however, prevention-focused employees have more positive voice behavior than promotion-focused employees. In addition, the leader-member exchange can moderate the relationship between regulatory foci and voice behavior.Originality/valueThis is an empirical study in the hotel field to examine the moderating effects of leader-member exchange on the relationships between regulatory foci and voice behavior. This research is contributed toward human resource management literature in the hospitality and tourism domain. Practices for managers and suggestions for future research are discussed.
Oil price shocks and emerging stock markets revisited
Authors: Das Debojyoti , Kannadhasan M , Bhattacharyya Malay 
Published: November 2020

PurposeThe study aims to understand the role of different streams of oil shocks (demand, supply and risk shocks) on the oil-importing and exporting countries' stock returns. The study also examines the impact of crude oil shocks across the economic regimes and market states. Besides, the role of the Global Financial Crisis (GFC) of 2008 in shaping the oil–stock relationship is also investigated.Design/methodology/approachThe authors revisit the impact of oil shocks on emerging equity markets by using the novel shock decomposition algorithm proposed by Ready (2018). The authors consider 24 emerging equity markets for the period spanning over July 15, 2002, to June 18, 2018, and bifurcate them based on oil dependence. The authors use rolling and dynamic conditional correlation analysis to understand the time-varying co-movements between oil prices and stock returns. The regime and state-specific dependence of stock returns on the structural oil shocks are captured by the Markov regime switching and quantile regression models.FindingsThe authors find that the demand shocks are positively associated with stock markets, whereas the supply shocks are negatively related, except in some of the oil-exporting countries. The risk-based shocks also appear to have a negative association with stocks. The authors do not find evidence of strong regime dependence and the direction of relationship across the high and low regimes is somewhat stable. Further, the authors observe an intense oil–stock relationship in the bearish market conditions. Besides, the authors also report evidences of changes in oil–stock relationship onset the GFC.Originality/valueThis is among the first studies to use the oil shock decomposition algorithm of Ready (2018) in the context of emerging equity markets. Additionally, oil shocks' role on the stock market movements across the regimes and market states is studied comprehensively. Thus, the nature of oil shock and the extent to which the emerging markets are exposed is observed in this study.
Implementing an intelligent video monitoring system to detect falls of older adults at home: a multiple case study
Authors: Lapierre Nolwenn , St-Arnaud Alain , Meunier Jean , Rousseau Jacqueline 
Published: November 2020

Purpose
Older adults are at a high risk of falling. The consequences of falls are worse when the person is unable to get up afterward. Thus, an intelligent video monitoring system (IVS) was developed to detect falls and send alerts to a respondent. This study aims to explore the implementation of the IVS at home.

Design/methodology/approach
A multiple case study was conducted with four dyads: older adults and informal caregivers. The IVS was implemented for two months at home. Perceptions of the IVS and technical variables were documented. Interviews were thematically analyzed, and technical data were descriptively analyzed.

Findings
The rate of false alarms was 0.35 per day. Participants had positive opinions of the IVS and mentioned its ease of use. They also made suggestions for improvement.

Originality/value
This study showed the feasibility of a two-month implementation of this IVS. Its development should be continued and tested with a larger experimental group.
Do policy and training changes influence patterns of police use of force? An interrupted time-series analysis
Authors: Rockwell Alexis Rain , Bishopp Stephen A. , Orrick Erin A. 
Published: November 2020

PurposeThe current study examines the effect of changing a specific use-of-force policy coupled with de-escalation training implementation on patterns of police use of force.Design/methodology/approachAn interrupted time-series analysis was used to examine changes in police use-of-force incident records gathered from a large, southwestern US metropolitan police department from 2013 to 2017 based on a TASER policy change and de-escalation training implementation mid-2015.FindingsResults demonstrate that changes to use-of-force policy regarding one type of force (i.e. use of TASERs) coinciding with de-escalation training influence the prevalence of use-of-force incidents by increasing the reported police use-of-force incidents after the changes were implemented. This finding is somewhat consistent with prior literature but not always in the desired direction.Practical implicationsWhen police departments make adjustments to use-of-force policies and/or trainings, unintended consequences may occur. Police administrators should measure policy and training outcomes under an evidence-based policing paradigm prior to making those adjustments.Originality/valueThis study is the first to measure the effects of changing use-of-force policy and implementing de-escalation techniques in training on patterns of police use of force and shows that these changes can have a ripple effect across types of force used by police officers.
Remaking Shanghai: New Divisions in an Expanding Metropolis
Authors: Gu Honghuan , Logan John R. , Wu Ruijun 
Published: November 2020

AbstractThis study of Shanghai analyzes the city's emerging patterns of residential settlement in 2010. Most previous research on China focused on central city patterns, but by 2010 urban development in major metropolitan areas was taking place predominantly in the suburbs. The analysis re‐examines and extends previous studies of the level of segregation by various key population characteristics, showing that the highest segregation is found in the suburban ring, where original villager residents are now joined by an influx of migrants from other regions and by intracity movers. We probe the sources of the segregation pattern in multivariate analyses at two scales—an innovative analysis at the level of individuals that shows how people's citizenship status, occupation and education affect the type of housing that they are able to live in, and jointly contribute to their location in the metropolitan area. We show that the patterns attributable to the market reform period mostly did not supplant the socialist urban structure, but rather used it as its foundation. Segregation today can be attributed less to current class inequality than to state policies in the distant and recent past that have determined when, where and for whom housing is built.
Introducing human papillomavirus (HPV) primary testing in the age of HPV vaccination: projected impact on colposcopy services in Wales
Authors: Pesola F , Rebolj M , Leeson S , Dunk L , Pickford L , Gjini A , Sasieni P 
Published: November 2020

ObjectiveTo determine the demand for colposcopy in the Cervical Screening Wales programme after the introduction of human papillomavirus (HPV) cervical screening, which coincided with the start of screening of women vaccinated against HPV types 16/18.DesignThe study used a computational model that assigns screening and screening‐related colposcopy events to birth cohorts in individual calendar years.SettingCervical Screening Wales.PopulationWomen aged 25–64 years from birth cohorts 1953–2007.Methods and main outcome measuresWe estimated the numbers of colposcopies and high‐grade cervical intraepithelial lesions (CIN2+) within Cervical Screening Wales in 2018–32, using official population projections for Wales and published estimates of the effects of HPV screening and vaccination.ResultsVaccination will reduce the number of colposcopies by 10% within the first 3–4 years after the national roll‐out of HPV screening, and by about 20% thereafter. The number of screening colposcopies is estimated to increase from 6100 in 2018 and peak at 8000 (+31%) in 2021, assuming current screening intervals are maintained. The numbers of CIN2+ lesions follow similar patterns, stabilising at around 1000 diagnoses per year by 2026, approximately 60% lower than at present. Extending the screening intervals to 5 years for all women shows similar trends but introduces peaks and troughs over the years.ConclusionsVaccination will not fully prevent an increase in colposcopies and detected CIN2+ lesions during the first 2–3 years of HPV‐based screening but the numbers are expected to decrease substantially after 5–6 years.Tweetable abstractHPV‐based cervical screening will initially increase colposcopy referral. In 6 years, this increase will be reversed, partly by HPV vaccination.
Effects of seasoning on the formation of heterocyclic amines and polycyclic aromatic hydrocarbons in meats: A meta‐analysis
Authors: Neves Thaís de Moura , da Cunha Diogo Thimoteo , de Rosso Veridiana Vera , Domene Semíramis Martins Álvares 
Published: November 2020

AbstractCooking improves digestibility, palatability, and microbiological profile of meats, but can produce compounds with carcinogenic potential, such as heterocyclic amines (HAs) and polycyclic aromatic hydrocarbons (PAHs). It has been shown that the formation of these compounds in meats can be inhibited by spiced marinades, but there is a complexity to check and compare the results of isolated studies with so many variables involved. Thus, this work aimed to review studies that evaluated the effects of spices on the formation of HAs and PAHs in meats according to cooking techniques and spice type. A meta‐analysis with a random effect model was conducted using response ratios (R) to identify and summarize previous results and answer the research question. The use of spices before frying (R* = 0.52), grilling (R* = 0.63), or roasting (R* = 0.74) meat, and spicing with garlic and onion (R* = 0.57), pepper (R* = 0.63), and other spices with phenolic compounds (R* = 0.63), decrease the formation of HAs and PAHs, due to the antioxidant and electron transfer capacity. In this article, we discuss how the improvement of culinary techniques with the dissemination of knowledge about meat preparation conditions is an effective strategy for reducing the formation and ingestion of HAs and PAHs; this is important due to the growing evidence about the association between meat consumption and chronic diseases. This is the first systematic review with meta‐analysis about this topic and can guide industry, food services, and population to improve the safety associated with meat consumption.
Ethnoveterinary for food‐producing animals and related food safety issues: A comprehensive overview about terpenes
Authors: Silva Jonas Joaquim Mangabeira da , Campanharo Sarah Chagas , Paschoal Jonas Augusto Rizzato 
Published: November 2020

AbstractAlternatives to the use of conventional veterinary drugs in food‐producing animals have gained attention, such as the use of natural products (NPs), mainly to soften the risks to the animal, the environment, and consumer's health. Although NPs have consistent advantages over conventional drugs, they cannot be considered risk free under food safety matters. In this way, this document presents a comprehensive overview of the importance of considering both the pharmacological and toxicological properties of the constituents of a NP from plants intending the standardization and regulation of its use in food‐producing animals. Terpenes are the most diverse class of natural substances present in NP of vegetal origin with a broad range of biological activities that can be explored in veterinary science; however, certain plants and terpenes also have significant toxic effects, a fact that can harm the health of animals and consequently generate economic losses and risks for humans. In this context, this review gathered scientific data of vegetal species of importance to ethnoveterinary for food‐producing animals, which produce terpenes, its biological effects, and their implications on food safety issues for consumers. For this, more than 300 documents were selected from different online scientific databases. The present data and discussion may contribute to the rational commercial exploration of this class of NPs in veterinary medicine.