bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reduction of Physical Activity Levels During the COVID-19 Pandemic Might Negatively Disturb Sleep Pattern
Authors: Diniz Tiego A. , Christofaro Diego G. D. , Tebar William R. , Cucato Gabriel G. , Botero João Paulo , Correia Marilia Almeida , Ritti-Dias Raphael M. , Lofrano-Prado Mara C. , Prado Wagner L. 
Published: December 2020

BackgroundThe outbreak of novel coronavirus disease 2019 (COVID-19) has caused a global panic and public concern due to its mortality ratio and lack of treatments/vaccines. Reduced levels of physical activity have been reported during the outbreak, affecting the normal daily pattern.ObjectiveTo investigate (i) the relationship of physical activity level with sleep quality and (ii) the effects of reduction physical activity levels on sleep quality.MethodsA Google form was used to address personal information, COVID-19 personal care, physical activity, and mental health of 1,907 adult volunteers. Binary logistic regression was used to verify the association of physical activity parameters and sleep quality.ResultsInsufficient physical activity levels were a risk factor to have disturbed sleep pattern [OR: 1.28, 95% CI (1.01–1.62)]; however, when the BMI was added to the analysis, there was no more statistical difference [OR: 1.23, 95% CI (0.96–1.57)]. On the other hand, we found that the reduction of physical activity levels was associated with negative changes in sleep quality [OR: 1.73, 95% CI (1.37–2.18)], regardless all the confounders [OR: 1.30, 95% CI (1.01–1.68)], unless when feeling of depression was added in Model 6 [OR: 1.28, 95% CI (0.99–1.66)].ConclusionDisruption in daily physical activity routine, rather than physical activity level, negatively influences sleep quality during the COVID-19 quarantine.
The Impact of Covid-19 Pandemic Lockdown During Spring 2020 on Personal Trainers' Working and Living Conditions
Authors: Bratland-Sanda Solfrid , Mathisen Therese Fostervold , Sundgot-Borgen Christine , Sundgot-Borgen Jorunn , Tangen Jan Ove 
Published: December 2020

Purpose:The aim of this study was to map changes in working and living conditions of Norwegian personal trainers (PTs) during the Covid-19 lockdown spring 2020.Methods:A total of 150 PTs (mean (SD) age 35.5 (8.4) years, with 6.5 (5.3) years of experience as PT, and 61% female) in Norway responded to an online survey.Results:Number of PT sessions per week was reduced during the lockdown and the majority of the respondents reported loss of clients and negative impact on their working conditions. The official restrictions and guidelines were considered difficult to interpret and somewhat discriminatory compared to restrictions and guidelines for other comparable exercise occupational groups. The lockdown period provided more PTs to offer online and outdoor training. Living conditions were also affected with reported impairments in private economy and reduced vitality (p&lt; 0.05), and with vitality being lower in female compared to male respondents during, but not before, the lockdown period.Discussion:The reported negative impact of the Covid-19 lockdown period on PTs' working and living conditions are discussed with focus on the reported gender differences and considering the previously reported poor reputation of the fitness industry.
Cellular Proliferation of Equine Bone Marrow- and Adipose Tissue-Derived Mesenchymal Stem Cells Decline With Increasing Donor Age
Authors: Bagge Jasmin , MacLeod James N. , Berg Lise C. 
Published: December 2020

Background: Bone marrow (BM)- and adipose tissue (AT)-derived mesenchymal stem cells (MSCs) are used increasingly for autologous cell therapy in equine practice to treat musculoskeletal and other injuries. Current recommendations often call for 10–100 million MSCs per treatment, necessitating the expansion of primary cells in culture prior to therapeutic use. Of concern, human and rodent studies have shown a decline of both MSC recovery from sampled tissue and in vitro proliferative capacity with increasing donor age. This may be problematic for applications of autologous cell-based therapies in the important equine demographic of older patients.Objectives: To investigate the effect of donor age on the cellular proliferation of equine BM- and AT-MSCs.Study Design:In vitro study.Methods: BM- and AT-MSCs and dermal fibroblasts (biological control) were harvested from horses in five different age groups (n = 4, N = 60); newborn (0 days), yearling (15–17 months), adult (5–8 years), middle-aged (12–18 years), and geriatric (≥22 years). Proliferation of the cells was tested using an EdU incorporation assay and steady state mRNA levels measured for targeted proliferation, aging, and senescence biomarkers.Results: The cellular proliferation of equine BM- and AT-MSCs declined significantly in the geriatric cohort relative to the younger age groups. Proliferation levels in the two MSC types were equally affected by donor age. Analysis of steady state mRNA levels showed an up-regulation in tumor suppressors, apoptotic genes, and multiple growth factors in MSCs from old horses, and a down-regulation of some pro-cycling genes with a few differences between cell types.Main Limitations: Potential age-dependent differences in cell function parameters relevant to cell-therapy application were not investigated.Conclusions: The cellular proliferation of equine BM- and AT-MSCs declined at advanced donor ages. High levels of in vitro proliferation were observed in both MSC types from horses in the age groups below 18 years of age.
Comorbidity in chronic kidney disease: a large cross-sectional study of prevalence in Scottish primary care
Authors: MacRae Clare , Mercer Stewart W , Guthrie Bruce , Henderson David 
Published: December 2020

BackgroundChronic kidney disease (CKD) is commonly comorbid with hypertension, diabetes, and cardiovascular disease (CVD). However, the extent of comorbidity in CKD across a range of concordant (shared pathophysiology and/or treatment) conditions and discordant (unrelated pathophysiology and/or different or contradictory treatment) conditions is not well documented.AimTo ascertain the prevalence of comorbidity, across 39 physical and mental health comorbidities, in adults with CKD in a large, nationally representative primary care population.Design and settingCross-sectional analysis of a primary care dataset representing 1 274 374 adults in Scotland.MethodThis study was a secondary analysis of general practice electronic medical record data using binary logistic regression models adjusted for age, sex, and socioeconomic status. Data of adults aged ≥25 years and 40 long-term conditions were used.ResultsA total of 98.2% of adults with CKD had at least one comorbidity, versus 51.8% in controls. After adjustment for age, sex, and deprivation, people with CKD were more likely to have 1 (adjusted odds ratio [aOR] 6.5, 95% confidence interval [CI] = 6.0 to 7.1), 2–3 (aOR 15.2, 95% CI = 14.0 to 16.5), 4–6 (odds ratio [OR] 26.6, 95% CI = 24.4 to 28.9), and ≥7 other conditions (OR 41.9, 95% CI = 38.3 to 45.8). Furthermore, all concordant (seven out of seven), the majority of discordant physical health conditions (17 out of 24), and mental health conditions (six out of eight) had statistically significant positive associations with CKD after adjustment.ConclusionChronic kidney disease is associated with extreme comorbidity across a wide range of mental and physical conditions. Routine care for people with CKD should include recognition and management of comorbidities, and clinical guidelines should support clinicians to do this.
A prospective study of the monitoring of patients with chronic myeloid leukemia upon withdrawal of tyrosine kinase inhibitor therapy
Authors: Turkina A. G. , Petrova A. N. , Chelysheva E. Yu. , Shukhov O. A. , Tsyba O. N. , Golenkov A. K. , Vysotskaia L. L. , Bykova A. V. , Nemchenko I. S. , Gusarova G. A. , Pospelova O. M. , Gurianova M. A. , Martynkevich I. S. , Abdullaev A. O. , Sudarikov A. B. , Kulikov S. M. , Savchenko V. G. 
Published: December 2020

Introduction. The advent of tyrosine kinase inhibitors (TKIs) in clinical practice drastically improved prognosis in patients with chronic myeloid leukaemia (CML). Adverse events of the TKI therapy and its high ﬁnancial burden warrant the trend to gradually abandon this treatment.Aim. To assess the results of CML patient monitoring after the withdrawal of TKI therapy.Patients and methods. This prospective study included 98 chronic phase CML patients satisfying the criteria: any receiving of TKI therapy for ≥3 years; deep molecular response (DMR, BCR-ABL ≤ 0.01 % IS) during ≥ 2 years. The withdrawal was followed by quantitative BCR-ABL estimation performed monthly for the ﬁrst 6 months of the survey, bimonthly for 1 year and every 3 months from the second year onwards. Therapy was resumed at a loss of major molecular response (MMR, BCR-ABL ≥ 0.1 % IS).Results. The MMR loss upon the TKI withdrawal was observed in 48 (49 %) patients. Survival without MMR loss was 52 % past 24 months since withdrawal, with a median of 35 months (23–52). The duration of therapy, MR and the MR depth at the time of withdrawal signiﬁcantly correlated with a conserved post-therapy MMR. Gender, age, a Sokal risk group, type and line of TKI therapy at withdrawal, and imatinib resistance in history were not observed to signiﬁcantly impact molecular relapse-free remission. MMR was recovered in all 48 patients with TKI therapy resumed in molecular relapse. In 65 % of the patients, adverse therapy events observed during treatment completely resolved by 6 months of post-therapy monitoring. Musculoskeletal pain (withdrawal syndrome, WS) was reported in 42 % patients in the post-therapeutic period, which did not lead to TKI resumption. The WS development correlated with an elder age and longer therapy prior to withdrawal.Conclusion. Molecular relapse-free survival in CML patients with treatment-free remission (TFR) is comparable to other published evidence. Monitoring safety during TFR is attested by the lack of disease progression and MMR recovery upon TKI resumption in all patients.
Oligoclonality and subpopulation structure of bone marrow T-cells in patients with aplastic anaemia
Authors: Abramova A. V. , Galtseva I. V. , Mikhailova E. A. , Kapranov N. M. , Davydova Yu. O. , Fidarova Z. T. , Troitskaya V. V. , Parovichnikova E. N. , Savchenko V. G. 
Published: December 2020

Introduction. The main pathogenetic mechanism of the development of aplastic anemia (AA) is a violation of the immune regulation of hematopoiesis.Aim: to study of the subpopulation composition of T-cells and the repertoire of the T-cell receptor in AA patients.Patients and Methods. The study included AA patients (n = 40) without prior immunosuppressive therapy in 2018–2020. The T-cell subpopulation structure and T-cell receptor Vβ-family (TCR-Vβ) oligoclonality were studied in samples of bone marrow using ﬂow cytometry.Results. We report characteristic properties of T-cell subpopulations of bone marrow in all AA patients: elevated counts of cytotoxic T-cells, effector CD4+ and CD8+ cells, CD4+ memory cells, which may suggest a long-term antigenic stimulation with subsequent activation of these cell subpopulations resulting in hyperexpression of pro-inﬂammatory cytokines. Diminishing of naive CD4+ and CD8+ cells, regulatory and double negative T-cells may indicate a relaxing control of cytokine-producing T-cells. A relationship has been established between the AA severity and counts of effector, regulatory, double negative and PD-1 positive T-cells. A highest count of potentially cytokine-producing T-cells and lowest count of cells involved in T-cell activity regulation were observed in very severe AA patients. Studies of the TCR-Vβ repertoire revealed oligoclonal expansion in the cytotoxic T-cell subpopulation.Conclusion. Enrichment in selected Vβ families suggests autoreactive T-cell clonality and attests to the immune nature of AA. A dynamic TCR-Vβ repertoire assay may be recommended in the disease monitoring. Flow cytometry helps identify valuable biomarkers for T-cell clone monitoring in AA and a better assessment of the disease progression.
Whole-body magnetic resonance imaging for diagnosis and treatment monitoring in multiple myeloma
Authors: Lutsik N. S. , Mendeleeva L. P. , Solovev M. V. , Kulikov S. M. , Chabaeva Yu. A. , Yatsyk G. A. 
Published: December 2020

Introduction. Whole-body diffusion-weighted magnetic resonance imaging (MRI) is an informative method for bone marrow inﬁltration diagnosis in patients with multiple myeloma (MM) and post-monitoring in autologous haematopoietic stem cell transplantation (auto-HSCT).Aim: to study bone marrow lesions in MM patients using whole-body MRI prior to and after chemotherapy with subsequent auto-HSCT.Materials and methods. Forty patients with MM were included in a prospective study of whole-body MRI before and after high-dose chemotherapy with auto-HSCT. All patients had whole-body MRI prior to and at +100 day of auto-HSCT. Antitumour response was assessed after induction and at +100 day. The number and volume of bone marrow lesions prior to and at +100 day of auto-HSCT were determined, along with apparent diffusion coefﬁcient (ADC) in the lesions.Results. We observed a signiﬁcant reduction of 29 % in the number of lesions, 40 % — in their volume and 33 % — in ADC. A signiﬁcant correlation was revealed between relative reduction in the number and volume of foci (r = 0.52, p = 0.0017). A correlation was found between relative reduction in the foci number and ADC (r = 0.47, p = 0.016). Patients with lesions > 7 cm3 in MRI data exhibited a lesser reduction in the foci number and volume and ADC values after auto-HSCT compared to patients with lesions < 7 cm3.Conclusion. Whole-body MRI with diffusion-weighted imaging and subsequent estimation of the number and volume of lesions and their ADC values prior to and after auto-HSCT add power to assessing antitumour response in MM patients with auto-HSCT.
Manfaat Jintan Hitam (Nigella sativa) sebagai Antiinflamasi dan Pereda Nyeri pada Osteoartritis (OA)
Authors: Widjaja Chanief Hassan 
Published: December 2020

Osteoartritis (OA) adalah salah satu penyakit yang sering dijumpai di berbagai penjuru dunia. OA sering mengenai wanita atau pria terutama pada mereka yang sudah berusia lanjut. Tidak jarang penderita OA sering mengeluhkan rasa nyeri yang hebat akibat penyakit tersebut. OA adalah penyakit tulang degeneratif yang sering dihubungkan dengan adanya kerusakan kartilago sendi pada penderita. Walaupun OA pada awalnya disebut sebagai penyakit sendi non inflamasi, beberapa studi menunjukkan bahwa OA dapat terjadi akibat adanya inflamasi kronis yang mendasari penyakit tersebut. Beberapa studi menunjukkan bahwa sitokin inflamasi IL-1β dan TNF-α berperan penting pada pathogenesis OA. OA yang tidak ditangani akan berujung pada disabilitas penderitanya. Nigella sativa atau jintan hitam adalah salah satu tanaman herbal yang sering digunakan sebagai obat antiinflamasi untuk berbagai penyakit, salah satunya adalah osteoarthritis. Nigella sativa memproduksi senyawa thymoquinine yang dapat berperan sebagai senyawa antiinflamasi dan antiproliferative pada OA. Metode penelitian yang digunakan untuk adalah studi literatur dari buku, jurnal nasional dan internasional yang diterbitkan pada rentang tahun 2010 hingga tahun 2020. Hasil studi menunjukkan bahwa Nigella sativa berperan sebagai antiinflamasi dan pereda nyeri pada pasien OA.
Komplikasi Muskuloskeletal pada Pasien Diabetes Melitus
Authors: Prakasa Dirga Yudha 
Published: December 2020

Latar belakang. Selain komplikasi makrovaskular dan mikrovaskuler, pasien diabetes mungkin mengalami beberapa komplikasi muskuloskeletal. Dampak komplikasi muskuloskeletal terhadap kualitas hidup penderita diabetes sangat besar. Terdapat banyak data epidemiologi yang mengaitkan komplikasi ini dengan diabetes, tetapi patogenesis dan faktor penyebab belum dijelaskan lebih lanjut. Oleh karena itu, tujuan dari studi literatur ini adalah untuk mengetahui lebih lanjut komplikasi muskuloskeletal pada pasien diabetes melitus. Metode. Studi ini merupakan literature review, dengan sumber pustaka yang digunakan sebanyak 22 pustaka, berasal dari 1 jurnal nasional dan 21 jurnal internasional. Hasil. Beberapa penelitian menunjukkan komplikasi muskuloskeletal yang sering dijumpai pada penderita diabetes melitus antara lain kapsulitis bahu, sindrom mobilitas sendi terbatas (LJM), kontraktur Dupuytren, carpal tunnel syndrome dan trigger finger. Kerusakan pembuluh dan saraf, glikosilasi protein, dan peningkatan kolagen di kulit dan jaringan ikat muskuloskeletal adalah beberapa faktor yang diduga berkontribusi dalam perkembangan gangguan muskuloskeletal pada penderita diabetes. Kesimpulan. Manifestasi muskuloskeletal dari diabetes sering ditemui, dan meskipun tidak mengancam nyawa, ia merupakan penyebab penting dari morbiditas, nyeri dan kecacatan.

Kata Kunci: diabetes melitus, komplikasi, muskuloskeletal
KARATERISTIK FESES AYAM KAMPUNG SUPER YANG DIBERI KUNYIT
Authors: Wahyudi Ferdi , Datau Fahria , Dako Safriyanto 
Published: December 2020

Tujuan penelitian ini adalah untuk mengetahui bagaimana penggunan kunyit dalam pakan ayam kampung super terhadap karateristik Feses yang dihasilkan. Sebanyak 120 ekor Ayam Kampung Super digunakan dalam penelitian ini. Periode perlakuan dilakukan pada hari ke-17 sampai hari ke-60. Kandang yang digunakan adalah personal cage, ukuran kandang 100 cm x 100 cm, sebanyak 20 unit dan masing masing cage. Rancangan penelitian menggunakan Rancangan Acak Lengkap (RAL) dengan 5 perlakuan dan 4 ulangan. Pemberian tepung kunyit pada pakan ayam kampung super tanpa kunyit (P0) menunjukan feses yang dihasilkan dominan bertekstur padat-cair, dengan warna feses putih kecoklatan dan bau yang sangat menyengat. Sedangkan pemberian pakan dan tepung kuyit hingga 6%, tekstur feses dominan bertekstur padat, warna feses coklat sedikit putih, hitam, dengan bau yang tidak menyengat. Terjadi penurunan pH feses berkisar 6.60 ( tanpa kunyit) menjadi 5.61 (diberi kunyit). Bobot feses setiap perlakuan adalah sama. Pemberian tepung kunyit hingga 6 % dalam bahan pakan mampu menurunkan pH feses, dan memperbaiki karateristik visual feses ayam Kampung Super
PERSONALIZED APPROACH TO ACUTE PERIANAL VENOUS THROMBOSIS TREATMENT DEPENDING ON CLINICAL MANIFESTATIONS
Authors: Garmanova T. N. , Markaryan D. R. , Kazachenko E. A. , Tsar'kov P. V. 
Published: December 2020

Aim: to investigate the time period and reasons for seeing a doctor of patients with acute thrombosis and to assess treatment satisfaction.Methods: The main complaints, symptom duration, pain severity and pathological process characteristics of patients with acute perianal venous thrombosis were recorded. The treatment strategy was determined by the doctor and the patient: the surgical thrombosed hemorrhoid removal or conservative treatment with analgesics, venotonics, drugs for stool softening. Pain severity on days 3, 7, 30 and overall satisfactions on day 30 were evaluated.Results: 62 patients were included. The main symptoms were pain (69.4%) and discomfort (16.1%). During the first 72 hours from the disease onset 21.5% of patients saw a doctor and 66.7% of all patients underwent the surgery, on day 4–7 — 50% saw a doctor and 77% underwent the surgery, > 8 days — 28.5% and 33% respectively. During the appointment pain was 4 points according to VAS, 3 days after surgery — 5.9 points, 4–7 days — 3.9 points, > 8 days — 2.5 points. 64% of patients underwent the surgery with average pain level of 5 points, in the conservative group — of 4.5 points (p = 0.014). On day 30 80.6% of all patients were completely satisfied. Having the pain severity > 4points nobody was satisfied with conservative treatment, while 80% of surgical patients were satisfied.Conclusion: Considering the symptom duration, pain continuing > 3 days, perianal discomfort ora «bump» the treatment tactics should be accepted by the doctor and the patient together for achieving a high treatment satisfaction.
CLINICAL-MORPHOLOGICAL CLASSIFICATION OF ACUTE THROMBOSIS OF THE EXTERNAL HEMORRHOID NODE IN RELATION TO A DIFFERENTIATED APPROACH IN ITS TREATMENT
Authors: Shchegolev A. I. , Sokolova A. A. , Trunin E. M. , Begishev O. B. , Sigua B. V. , Gainullina R. F. 
Published: December 2020

Acute thrombosis of the external hemorrhoid node is one of the common manifestations of hemorrhoids and a frequent reason for seeking specialized medical care. Despite existing prospective studies proving the effectiveness of operative treatment, there is no clear position in determining the indications, timing and methods of surgery, nor the combination of the latter with conservative therapy.The article formulated and described the original clinical-morphological classification of acute thrombosis of the external hemorrhoid node based on the data of its ultrasound examination (ultrasound). Effectiveness of various methods of treating acute thrombobosis of external hemorrhoid node, carried out on the basis of proposed classification, is evaluated.Purpose of the study. Improving treatment outcomes for patients with acute thrombosis of the external hemorrhoid node.Results. Ultrasound types of thrombosis of external hemorrhoidal node are revealed:– mononuclear (in ultrasound, it is a single thrombus or a dense, intimately soldered group of thrombotic masses of an an- or isoechogenic structure with untested swelling of the skin and subcutaneous fat (PWD), from 10 mm or more in size);– polynuclear (clusters of thrombotic masses of an- or isoechogenic structure up to 10 mm in size, located at a short distance from each other, with moderate swelling of the skin and subcutaneous fat);– edema (during sonography, multiple small, up to 2–3 mm, anechogenic thrombotic masses are visualized against the background of pronounced edema of subcutaneous fat fiber and skin).Effectiveness of various methods of treating patients with acute thrombosis of external hemorrhoid node in groups formed on the basis of proposed classification is evaluated. It has been found that in patients with edematous sonographic type of thrombosis, it is more advisable to apply only conservative therapy or carry out surgical treatment after completion of taking diosmin-containing preparations. Such tactics reduced the duration of treatment and reduced pain syndrome. In patients with a mononuclear type of thrombosis of the external hemorrhoidal node, a similar result was noted during surgery and the beginning of conservative therapy on the day of circulation.Conclusion. Application of ultrasound diagnostics allows establishing the type of hemorrhoid node thrombosis and using the obtained data when choosing treatment tactics. A differentiated approach to treating this category of patients, based on the proposed classification, allows improving the treatment results of patients with acute thrombosis of the external hemorrhoid node.