bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of Bacillus spp. and Nitrosomonas sp. in commercial feed as a probiotic agent to increase growth performance and feed efficiency of sangkuriang satfish (Clarias gariepinus)
Authors: Yaqin A A , Sudarno  , Rozi  
Published: February 2020

Abstract
The purpose of this research was to know the combination effect of Bacillus spp., Nitrosomonas sp., that added to the commercial feed to feed conversion and growth on Sangkuriang Catfish. The method used in this experiment was design with 3 treatments and 3 replications. Samples (24.49±1.21 g) were a stocking density of 1,500 fish/pond and maintenanced for 30 days. The treatment applied was P0 treatment (feed without probiotic), P1 (5.0 x 107 CFU/gram feed of probiotic was sprayed), and treatment P2 (5.0 x 109 CFU/gram feed of probiotic is sprayed). The best treatment was probiotic in treatment P1 quantitatively made a real effect in growth and made a better feed efficiency than other treatments, with FCR (0.7±0.57), SGR (4.33±0.11), EPP (109.61±9.69), SR (96.91±0.45), DWG (2.07±0.18). Water quality of culture media was in the range of reasonable level of cultivation C. gariepinus. Adding probiotic P1 could be an effective alternative to increase growth performance and feed efficiency on C. gariepinus.
The Percentage of embryo viability after 48h sperm cryopreservation: effect of various natural cryoprotectant
Authors: Lestari S , Abinawanto  , Bowolaksono A , Gustiano R , Kristanto A H 
Published: February 2020

Abstract
Natural cryoprotectant is an important factor needed to protect cell compound. Its proper usage is based on toxicity which is influenced by its type, concentration, temperature, and exposure period of the compound. This study aims to evaluate the best concentration period of natural cryoprotectant, with the utilized sperm obtained from Tor soro fish using the stripping technique. It was further diluted with a solution consisting of fish ringer, methanol 10%, and natural cryoprotectant in a ratio of 1:10. The natural cryoprotectant used were honey, brown sugar, and date palm juice, at 10 % concentration. Furthermore, the sperm was stored in the liquid nitrogen at -196 °C for 48 h, melted at 36 °C for 1 minute, and mixed with 100 eggs for fertilization. The percentage of embryo viability was analyzed using ANOVA and Tukey test. The result showed an effect of natural cryoprotectant on frozen sperm towards the percentage of embryo viability (p<0.05). The honey cryoprotectant is a natural compound that produces the highest embryo viability percentage at 1080 minutes after fertilization with a total of 94.20 ± 2.79 (p<0.05).
Effect of different bacterial strain in probiotics on the growth performance of Nile Tilapia (Oreochromis niloticus)
Authors: Sholihuddin T D , Arief M , Kenconojati H 
Published: February 2020

Abstract
The main purpose of this study was to investigate the effect of different bacterial type in probiotics on the growth performance of Nile tilapia. In this study, commercial probiotics with a different type of bacteria were added to the diet. The first group, as control, was fed with a commercial diet without probiotic. The second group was fed with commercial diet plus probiotic X contained Lactobacillus sp., Bacillus sp. and Pseudomonas sp. The third group was applied commercial diet plus probiotic Y contained Lactobacillus sp., Bacillus sp. and Bifidobacterium sp. Feeding trial was performed for 30 days. The result showed that the growth performance of Nile Tilapia including weight gain (WG) and specific growth rate (SGR) was significantly higher than the control group (P<0.05). The addition of probiotics in the commercial diet also improved feed utilization significantly. The best growth performance was obtained in the third group where the probiotic Y applied. Different bacterial strain in probiotic could give a different effect on the growth performance of Nile Tilapia because of their different action mode in fish. Therefore, the appropriate selection of probiotic strains can lead to specific advantages for species in aquaculture.
Morphology, morphometrics, and some qualitative parameters of silver rasbora (Rasbora argyrotaenia) sperm
Authors: Adawiyah L A , Sulmartiwi L , Budi D S 
Published: February 2020

Abstract
The aims of this study are to inform the morphology, morphometrics, and some qualitative parameters of silver rasbora (Rasbora argyrotaenia) sperm as the knowledge that can be used as a basis for further research. This study was conducted in March until April 2018 in Wet Laboratory and Instrument Laboratory Universitas Airlangga, Banyuwangi Campus, Indonesia. A total of 10 male of silver rasbora with a weight of 4.11 ± 0.31g and length 7.78 ± 0.20 cm was induced using Ovaprim™ at a dose of 0.35 mL/g body weight to stimulate spermiation. Abdominal gentle stripping was done after 10 hours to collect sperm. The observed parameters are sperm morphology, head length and tail length as morphometric parameters, thickness, colour, and smell. Morphological description based on observation; a sperm consists of an oval head, a cylindrical midpiece, and a thin elongated tail. Averages of head length and tail length are 1.64 ± 0.25 mm and 30.57 ± 2.87 mm. Some qualitative parameters of sperm were observed include thick consistency, milky white colour, and fishy odour.
Detection potential fishing zones of Longtail tuna (Thunnus tonggol) using fisheries and remotely sensed data in the waters around Madura Island
Authors: Syah A F , Sholeh M 
Published: February 2020

Abstract
In situ data of Longtail tuna fishing location and satellite-based oceanographic data of chlorophyll-a concentration (chl-a), sea-surface temperature (SST), and salinity were employed to figure out the impacts of oceanographic condition on the pattern of potential fishing zones for Longtail tuna in waters around Madura Island. The relation of those parameters to the dissemination of Longtail tuna was analyzed with a maximum entropy (Maxent). The predictive model performance was estimated using the area under the curve (AUC). The jackknife test was then employed to examine each parameter’s model contribution. The results from the Maxent model exhibited a high predictive success of the model with AUC value of 0.932. Maxent prediction showed a high probability occurrence of Longtail tuna occurred in the offshore area of the Java Sea. The results also revealed that the Longtail tuna habitat selection was significantly controlled by the salinity ranges from 32.3 – 32.5 ppt, SST ranges from 29.5–30.5°C, and chl-a ranges from 0.2 – 0.4 mg/m3. Besides, among the set of oceanographic variables utilized (SST and chl-a), salinity exhibited the highest value of the jackknife test. Therefore salinity expressed to be the most significant factor in the geographic dissemination of Longtail tuna in the waters Madura Island.
Evaluation of aqueous extract of robusta coffee (Coffea canephora) leaves for controlling Argulus japonicus infestation on common carp seed
Authors: Afifah N , Kismiyati  , Kenconojati H 
Published: February 2020

AbstractArgulus japonicusis common crustacea ectoparasite that infest in freshwater fish. The heavy infestation may trigger other fish pathogen infection which lead to mass mortality. Common carp seed has been known as the most vulnerable host forA. japonicusinfestation due to their immature immune system. Generally,A. japonicusinfestation could be controlled by chemical pesticides but its long-term use could create a harmful effect on the environment. Therefore, an eco-friendly natural product is needed to replace chemical pesticide. The purpose of this study was to evaluate the effectivity of water extract of robusta coffee leaves to controlA. japonicusinfestation. Water extract of robusta coffee leaves was obtained from decoction method. The fish were infected withA. japonicusby artificial infestation using cohabitation method. The infected fish were dipped at different concentration 400 (P1), 800 (P2), 1200 (P3) and 1600 ppm (P4) of water extract for 30 minutes. The result presented that the water extract of robusta coffee leaves was effective to controlA. japonicusinfestation in 1600 ppm. This study revealed that robusta coffee leaves have anti-parasitic activity againstA. japonicus. Hence, it can be used as an alternative natural product for controllingA. japonicusinfestation in fish.
The different effects of heat shock duration and initial period on hatching rate, abnormality rate, egg yolk absorption, and survival rate of spotted barb (Puntius binotatus) larvae
Authors: Mukti A T , Ahmadi M , Widjiati  , Luqman E M 
Published: February 2020

AbstractSpotted barb (Puntius binotatus) is one of Indonesian endemic fish with high economic value. However, this fish has slow growth, so innovative technology was required to increase fish growth. This study aimed to determine the different effects of heat shock duration and initial period on hatching rate, abnormality rate, length of egg yolk absorption, and survival rate of spotted barb larvae. This study was used an experimental method using factorial completely randomized design, with two factors of treatment, namely heat shock duration (1, 1.5, and 2 min) and initial period (3, 4, and 5 min after fertilization) of fish embryos. Egg fertilization was conducted artificially. The fish embryo was shocked at the temperature of 40ºC. Parameters of hatching and abnormality rates, length of egg yolk absorption period, and survival rate were observed. Data were analyzed statistically using analysis of variance followed Duncan’s multiple range test with a confidence level of 95%. The results showed that the fertilization rate of the spotted barb was above 81%. Treatment of heat shock duration and initial period influenced hatching rate of fish embryo, and abnormality and survival rates of larvae; however, treatments have similar lengths of egg yolk absorption in spotted barb larvae.
Blood glucose and digestive tract andoparasite helminth infection of cantang grouper (Epinephelus lanceolatus x Ephelus fuscoguttatus) from traditional ponds in the Kampung Kerapu of Lamongan East Java
Authors: Angwarmas B , Sulmartiwi L , Mahasri G , Rahayu N D , Pamenang G D 
Published: February 2020

Abstract
Cantang grouper Epinephelus fuscoguttatus x Epinephelus lanceolatus (E. fuscoguttatus x E. lanceolatus) is a hybrid fish between female tiger grouper and male kertang grouper, which has a high resistance to the environment and pathogen infection. The aim of this research was to find out the stress level (blood glucose levels) and helminth infections in the digestive tract of the cantang grouper (E. fuscoguttatus x E. lanceolatus) on a traditional pond in Kampung Kerapu Lamongan, East Java. This research is a survey research with 40 samples of Cantang grouper with purposive sampling. The results of the study showed that the Cantang groupers were reared in traditional ponds, positively infected by a single worms Prosorhynchus, with a prevalence of 24.4%. The lowest blood glucose levels was 0 mg/dL and the highest was 117 mg/dL. Infection was occurred in Cantang groupers that had blood glucose levels were below of normal, and above of normal with moderate infection degrees. The results also showed that there was no close correlation between blood glucose level of the Cantang grouper with intestinal helminth infections.
Inventory of ectoparasite helminth on the Hybrid Grouper (Epinephelus fuscoguttatus x Epinephelus lanceolatus) from traditional ponds in the Kampung Kerapu Lamongan East Java Indonesia
Authors: Rahayu N D , Sulmartiwi L , Mahasri G , Muntalim  , Angwarmas B , Pamenang G D 
Published: February 2020

Abstract
The purpose of this study was to determine ectoparasite helminth species, predilection, prevalence and intensity of attacks on Hybrid Grouper “Cantang” (Epinephelus fuscoguttatus x Epinephelus lanceolatus) from traditional ponds in Kampung Kerapu, Lamongan. This research is a survey with descriptive method. Hybrid Grouper “Cantang” samples were taken as many as 45 fish. Observations were made at the Anatomy and Fish Culture Laboratory, and Microbiology Laboratory of the Faculty of Fisheries and Marine Airlangga University, Surabaya. The result of this study, 25 of 45 Grouper were known to be infested. The ectoparasite helminth which infest the Groupers is Zeylanicobdella arugamensis which is a group of marine leech with a prevalence of 55.56 % so that it belongs to the very often category and the intensity of 11.32 ind/fish is classified in the moderate category. The distribution of Zeylanicobdella arugamensis predilection which infested Groupers was found most on the fins, 162 individual or 57.24 %.
Different Concentration of Rice Bran Suspension on Fecundity and Offspring Production of Each Moina macrocopa Broodstock
Authors: Ullimaz A , Nindarwi D D , Mubarak A S 
Published: February 2020

AbstractM. macrocopaculture success is influenced by the quality and quantity of inoculant, media, and feed used. Rice bran contains protein, lipid, amino acids, and vitamin B that supportsM. macrocopareproduction. This study aimed to determine the relationship of different rice bran suspension concentration against the reproduction of M. Macrocopa and the optimum concentration of bran suspension that produced the highest fecundity and offspring production level. This study was conducted using completely randomized design experimental method containing six treatments with four replications, i.e 22 mg/L (P0), 29.7 mg/L (P1), 39.6 mg/L (P2), 49.5 mg/L (P3), 59.4 mg/L (P4), and 69.3 mg/L (P5). Results showed that different suspension concentrations of rice bran inM. macrocopainfluenced significantly (p < 0.05) against the fecundity and offspring production on each broodstock with the optimum rice bran suspension concentration of 69.3 mg/L, as it produced the highest fecundity and offspring production level ofM. macrocopa.
Biological activities of Indonesian mangroves obtained by subcritical water extraction
Authors: Pangestuti Ratih , Siahaan Evi Amelia , Untari Febriana , Chun Byung Soo 
Published: February 2020

Abstract
Mangroves are still considered as underexploited marine resources in Indonesia, and functional materials from Indonesian mangroves are not characterized yet. In this study, different part of mangroves (Sonneratia alba leaves - SAL, Sonneratia alba roots - SAR, Sonneratia alba fruit - SAF, Rhizhopora mucronata - RMF, Rhizhopora lamarckii - RLF) were hydrolyzed using the subcritical water extraction (SCWE) system at 120 ºC. Mangroves hydrolysates (SAL, SAR, SAF, RMF and RLF) were further analyzed for total protein, sugar, reducing sugar, saponin and flavonoid content. The antioxidant capacity and functional material contents including total sugar, reducing sugar, protein, flavonoid and saponin contents. The highest flavonoid and saponin contents were obtained from RLF with the value of 20.13±0.17 mg/g and 31.83±0.18 mg/g, respectively. The antioxidant capacity and antibacterial properties of mangroves hydrolysates varied significantly based on the sample materials, with RLF showing the highest total antioxidant activity and antibacterial activity. The results of our study suggest that tropical mangroves especially R. lamarckii could be valorized efficiently, as a source of bioactive material using subcritical water extraction
Viability of Tor fish spermatozoa (Tor soro, Valenciennes 1842) 48-hours cryopreservation: the effects of duck egg yolk as a natural cryoprotectant
Authors: Wulandari P D , Abinawanto  , Subagja J , Kristanto A H 
Published: February 2020

Abstract
Tor is endemic freshwater fish in Sunda, locally known as kancra or dewa, which inhabits rivers with clean and clear water quality. In local customary and cultural perspectives, it is sacred fish whose populations have persistently depleted into scarcity. Sperm cryopreservation is one of many techniques to maintain its survival from extinction by inducing breeding at the reproductive stage. This study was designed to determine the effects of duck egg yolk as a cryoprotectant on the viability of Tor fish spermatozoa after 48 hours of cryopreservation. It employed the stripping method to obtain the fish semen and used diluent solution consisting of fish ringer solution, 10% methanol, and duck egg yolk in various concentrations (0%, 5%, 10%, 15%, 20%, 25%), with 1:10 ratio of sperm to diluent solution. These samples were equilibrated at 5°C for 10 minutes, frozen at -10°C for 48 hours in a freezer, and then defrosted at 40°C for 1 minute. The One-way ANOVA revealed statistically significant differences (p <0.05), and the post-hoc Tukey test also showed statistically significant differences (p <0.05). Because the 10% duck egg yolk produced the highest viability (averagely 83.75±1.71%), this concentration is the best cryoprotectant for optimal Tor spermatozoa cryopreservation.
Probiotic enriched Daphnia sp: the nutritional profile and enzymatic activities
Authors: Riyani E , Yuniarti A , Hariati A M 
Published: February 2020

Abstract
Live food played an important role in aquaculture especially hatcheries. This study was carried out to evaluate the effect of enriched different probiotic doses on total lenght, nutritional profile and enzymatic activities of Daphnia sp. This research used 3 treatments and 3 replications. The experimental treatments are 0, 5 and 10 ml L−1 and enriched period was 0, 12 and 24 hours. Probiotic consortia bacteria of Bacillus subtilis (107 CFU ml−1) and Lactobacillus casei (107 CFU ml−1) which is fermented by the addition of spices and molasses. Daphnia sp. was cultured in fresh water with a density of 20 ind ml−1. Observed parameters include total lenght, nutritional profile and enzymatic activities. The results showed that probiotic doses effect significantly different (p<0.05). In this research, the best performance enriched at 10 ml L−1 doses of probiotic and 12 hours incubation period included total lenght of 1.8 mm, and moisture, protein, lipid, ash amounted to 98.43±0.03, 42.72±0.3, 6.34±0.07, 11.72±0.39 %, protease and lipase activity were 21.92±0.90 U ml−1 and 0.82±0.06 U ml−1. Enriched Daphnia sp. has live food potential for hatcheries.
Enrichment of feed for growth of cantang grouper (E. fuscoguttatus x E. lanceolatus) in floating cages
Authors: Afifah D D , Satyantini W H , Mukti A T , Cahyoko Y 
Published: February 2020

Abstract
Grouper (Epinephelus sp.) is one of the superior species in the development of marine culture in Indonesia which has high economic value and has become an export commodity. One of the factors that influence production results in aquaculture is feed. Good quality of feed is crucial for fish survival and growth. Therefore, the feed given to cultured fish must be able to meet all the nutrients needed. One way that can be used to improve nutrition in feed is enrichment. This study aims to determine the enrichment of feed on the growth of cantang grouper by addition multivitamins to the feed. Cantang grouper reared in two floating net cages; one is given artificial feed without enrichment and the other one with multivitamin enrichment. The multivitamin dose for enrichment is 0.3%. Sampling was carried out by measuring the weight and length of 10 fishes every seven days from each floating net cage. The growth of cantang grouper which was given a multivitamin enrichment in the feed showed a growth rate 2.53 grams/day, length of growth 0.055 cm/day, feed efficiency was 29.4% higher and FCR was 3.4 lower compared to the grouper without enriched feed.
Analysis degradation of mangrove vegetation in Tangerang District, Banten Province
Authors: Haryanti R , Fahrudin A , Susanto H A 
Published: February 2020

Abstrak
One of the main coastal ecosystems of Tangerang Regency is the mangrove ecosystem is the mangrove ecosystem has numerous functions. However, this does not guarantee that it would not experience degradation and damage. Therefore, this study aims to analyse the level of mangrove ecosystem degradation in Tangerang Regency. The method used in this study is purposive sampling, whilst the measurement was performed through transect and non-transect methods with a reference to Indonesian National Standard Number 7717 year 2011. Data analysis in this study refers to calculations based on the Decree of the State Minister of Environment Number 201 year 2004 and Fauzi and Anna’s study in 2005. Results of this study indicated that the level of mangrove density of all village sites/stations in the category of rarely-damaged due to the fact that density values in all villages are below the range of 1,000 trees/ha. Meanwhile, the percentage value of mangrove cover is under the category of rarely-damaged to tightly-good. Even so, observation areas in Tangerang Regency are dominated by mangrove conditions in a rarely-damaged category. According to the analysis of the calculation of degradation level indicated mangrove ecosystem in Tangerang give trend in degradation categories.
Determination spesies flying fishes (exocoetidae) in makassar strait
Authors: Indrayani  , Sambah A B , Kurniawan A , Pariakan A , Jufri A , Wiadnya D G R 
Published: February 2020

Abstract

Exocoetidae have valid name 71 species, in Indonesia we found 18 species Makassar Strait is a potential area for catching flying fish. The purpose of this study was to determine the composition and distribution of flying fish species captured by gillnet. Flying fish data collection is conducted from March to May 2019. There are six species of flying fish collected. The results showed that in Makassar Strait, the species could be an identified of morphologic of flying fish is Hirundichthys oxycephalus, Cypselurus poecilopterus, Cheilopogon abeia, Cheilopogon spilopterus. Flyingfish was obtaining at latitude 117045’-118030’ ‘BT and 4030’BT - 3005’ LS during the study. There are expected to be reference literature as primary data for the management and sustainable use of flying fish in the Makassar Strait.
Potential stock of stony corals in Indonesia
Authors: Hadi T A , Giyanto  , Siringoringo R M , Budiyanto A , Johan O , Souhoka J , Abrar M , Sari N W P , Sadarun B , Prayudha B , Sutiadi R , Dzumalek A R , Sulha S , Suharsono  
Published: February 2020

Abstract
Indonesia is part of the coral triangle region which is known as the world’s highest marine biodiversity, including stony corals. This situation used to benefit local people by trading ornamental corals. The aims of this study are to investigate the potential stock of ornamental corals as well as the reef conditions. The study took place in Kendari, Luwuk, Sumbawa and Belitung where the ornamental coral-collecting activities used to occur. The result indicates there were approximately 110 species of corals found with the majority of coral status are common (harvest limited) and uncommon (harvest with cautions). Based on the juvenile existence, only 66 corals have potential stocks. Euphyllidae (Euphyllia glabrescens and Plerogyra sinousa) had the highest potential stock in Kendari and Luwuk, while Fungiidae (Fungia spp) held the highest potential stock in Belitung and Sumbawa. This difference is likely related to different locality or habitat type. In general, coral reefs in Kendari, Luwuk and Belitung are in good condition, while fair condition occurs in Sumbawa. To conclude, the stock of ornamental corals is still high and the reefs are generally good, suggesting the collecting activities may be allowed with proper quota and continuous monitoring habitat condition to ensure the sustainability.