bluuebunny/crossref_metadata_2025_split huggingface dataset:
Laser-wound stimulated adventitious root formation of Rosa canina cuttings involves a complex response at plant hormonal and metabolic level
Authors: Morales Orellana Raul Javier , Rath Thomas , Druege Uwe , Tandrón Moya Yudelsy A. , von Wirén Nicolaus , Winkelmann Traud 
Published: December 2024

IntroductionThe presence of wounds in addition to the excision-induced wounds after severance from the stock plants is known to positively influence adventitious root formation of woody plant cuttings. Previous morphological studies highlighted laser wounding as a technique allowing to precisely control the decisive ablation depth. However, the biochemical processes involved in the response of rooting to the additional wounding remained unexplored.MethodsThe present study analyzed changes in the plant hormone and carbohydrate profiles in response to laser treatments of rose leafy single-node stem cuttings (Rosa canina ‘Pfänder’). Concentrations of four groups of plant hormones and of carbohydrates were monitored in three different stem sections of the cutting base during the first eight days after excision of cuttings. In addition, histology was employed to investigate anatomical changes at the basal wound and the laser wounds at the start and the end of the experiment after 40 days.ResultsLaser ablation caused an increase of vascular tissue dimension directly in the laser wound, and increased the quantity and quality of rooting compared to control cuttings. A clear early local rise of jasmonic acid (JA) was detected directly in wounded areas after laser marking, as well as an increase in abscisic acid (ABA) that persisted for the subsequent days. Indole-3-acetic acid (IAA) levels were relatively high on day zero, but decreased thereafter. Interestingly, higher IAA levels were maintained in the stem section below the axillary bud compared with the opposite section. Laser-treated cuttings presented a clear increase in contents of IAA-amino acid conjugates (IAAGlu and IAAsp) and the oxidation product OxIAA. Differences in concentration of these IAA metabolites were related to the position of the laser wound relative to the axillary bud and leaf. Additionally, laser treatments caused gradually increased levels of the cytokinin N6-isopentenyladenine (iP) in laser-treated zones, and of zeatin riboside specifically when the laser wound was placed on the leaf-bud side. Additional laser wounding reduced starch and sucrose levels in all wounded sections at the end of the evaluation period, independently of the wounding location.DiscussionThe results of this study indicate that presence of additional injured tissue triggers a complex biochemical adjustment at the base of the cutting responsible of inducing vascular tissue growth and capable of generating a positive response to adventitious root formation.
The relationship between adolescent sleep duration and exposure to school bullying: the masking effect of depressive symptoms
Authors: Zeng Rushuang , Han Dang , Du Wei , Wen Jing , Zhang Youxian , Li Zongyu , Du Qun , Qi Yan , Li Yu , He Jia 
Published: December 2024

BackgroundAdolescents who suffer from school bullying are often accompanied by problems such as sleep disorders and depression. However, it remains unclear how depressive symptoms and sleep assessments such as sleep duration interact in the specific mechanisms of exposure to school bullying.ObjectiveTo understand the role of sleep duration, depressive symptoms on school bullying in adolescents and the mediating role of sleep duration in this context.MethodsA total of 1730 adolescents were selected from Xinjiang province, China by stratified cluster random sampling in 2020, and their demographic characteristics, exposure to school bullying, depressive symptoms, and sleep duration were investigated by questionnaire. Multifactorial logistics regression analyses were performed to examine the effects of sleep duration and depressive symptoms on school bullying. Furthermore, the bootstrap method was used to explore the mediating effect and masking effect of depressive symptoms between sleep duration and school bullying by PROCESS macro in SPSS 26.0.ResultsA total of 16.42% of adolescents suffered from school bullying and 12.25% showed depressive symptoms. Multifactorial logistics regression analyses revealed that possible depression and depression increase the risk of exposure to school bullying compared to normal group. Sleep duration less than 8 h and between 8 and 10 h are protective factors for exposure to three types of school bullying relative to those who sleep more than 10 h. Additionally, sleep duration affected exposure to school bullying through depressive symptoms (β = 0.011) and depressive symptoms masked the effect between sleep duration and exposure to school bullying with an effect of 60.17%. The masking effect remained stable after adjusting for gender, age, ethnicity, body mass index, and exercise intensity (indirect effect = −0.017, 95%CI: −0.026 to −0.009).ConclusionDepressive symptoms may be a risk factor for exposure to school bullying, while moderate and inadequate sleep duration may be protective factors for exposure to all three types of school bullying simultaneously. The effect of sleep duration on adolescents’ exposure to school bullying was affected by the masking effect of depressive symptoms, and appropriate sleep duration and a positive and healthy mindset were beneficial in reducing the occurrence of school bullying.
Understanding mental health challenges and associated risk factors of post-natural disasters in Bangladesh: a systematic review
Authors: Karim Md. Zawadul , Al-Mamun Md. , Eva Maliha Azad , Ali Md. Hazrat , Kalam Abul , Uzzal Nurul Islam , Das Pranto Kumer 
Published: December 2024

Background and objectivesNatural disasters are harmful occurrences caused by the Earth's geological and meteorological processes. Bangladesh is recognized as one of the country's most vulnerable to natural disasters. Therefore, the people of Bangladesh remain at high risk of natural disasters. However, no study has been conducted in Bangladesh to provide an overall perspective on mental health issues that arise after natural disasters. Thus, this systematic review aims to identify all mental health issues and related risk factors brought on by natural disasters in Bangladesh.Study designSystematic review.MethodsBetween April to May 2024, a systematic search was conducted across many databases, including PubMed, following the PRISMA guideline. Ultimately, 17 publications were included in this study.ResultsThe findings reveal that depression, anxiety, stress, suicidal ideation, post-traumatic stress disorder, major depressive disorder, generalized anxiety disorder and sleep disturbances are found as major risk factors for natural disasters. Prevalence of depression, anxiety and, stress, suicidal ideation ranges from 16.3% to 92.71%, 6% to 92.19%, 32.41% to 58%, 10.9% to 57.5% among people as consequences of natural disasters like heatwaves, cyclones, flood, river bank erosion etc. There are some associated risk factors which provoke prominent mental health problems, including (i) Demographic factors (age, gender, marital status, number of children, lower education, living location, living infrastructure, family conflicts, family member death, land for cultivation, loss of domestic animals), (ii) socio-economic factors (environmental settings, social support, disaster warning system, displacement, food crisis, financial support, income loss, vulnerable livelihoods) (iii) behavior and health-related factors (behavior pattern, physical injury during natural disaster, physical disability), (iv) other factors (increased temperature, living together, lower social class). People affected by natural disasters are often viewed in terms of their physical damage, while their mental health is always ignored.ConclusionCalculative measures are needed to create an overall picture of the effect of natural disasters on people's mental health in Bangladesh. Therefore, the government needs to consider establishing potential measures to lessen the impact of natural disasters on people's mental health.
Early numerical skills and mathematical domains in autistic students in primary school
Authors: Bejarano-Martín Álvaro , Casado-Vara Roberto , Magán-Maganto María , Díez Emiliano , Jenaro Cristina , Flores Noelia , Orrantia Josetxu , Canal-Bedia Ricardo 
Published: December 2024

IntroductionIt is crucial to provide a quality educational response to the needs of autistic children across various mathematical domains. However, there is no consensus on which of the early skills have the greatest predictive effect in the short and long term within these domains. Therefore, this research aimed to a) compare early numerical skills and mathematics domains, and 2) analyze the predictive value of early numerical skills into mathematics domains.MethodsForty-two children (twenty-one autistic children and twenty-one non-autistic children) aged 6-12 years participated in the study. Three areas were evaluated through different tasks: 1) control tasks: reading, impulse control and manual speed, 2) early numerical skills: counting, verbal subitizing, magnitude comparison and estimation, and 3) mathematical domains: arithmetic calculation and arithmetic word problems.ResultsSignificant differences were found in subitizing and estimation tasks. Both groups showed similar mathematical skills in arithmetic calculation and arithmetic word problems. For autistic students, several non-symbolic tasks predict performance in mathematical domains, whereas for non-autistic students, symbolic tasks were predictors.DiscussionAlthough mathematics does not seem to be an area of concern for autistic children, future studies should explore early numerical and mathematical domains in children with cognitive support needs through longitudinal research.
COVID-19 and social distancing: pandemic has altered social relationships and contacts in older adults over 4 years
Authors: Kastner Lydia , Suenkel Ulrike , von Thaler Anna-Katharina , Eschweiler Gerhard W. , Dankowski Theresa , Mychajliw Christian , Brockmann Kathrin , Heinzel Sebastian , Thiel Ansgar 
Published: December 2024

IntroductionSocial isolation is a main risk factor for loneliness, health issues and psychological diseases. With its restriction measures, the coronavirus pandemic has led to an objective reduction in meaningful interactions, communication, and social contacts in general (social isolation). However, it has been shown that older adults cope differently with social isolation. Therefore, the aim of the present study was to investigate the changes of social contacts of older adults over the pandemic period of 4 years.MethodsFor this purpose, N = 175 older adults (Mage = 72.60, SDage = 6.12 years, Mdnage = 72, Range: 60–87 years) were asked at 3 time points (2019, 2021, 2023) with how many people they had contact in the reference month (May, November). In addition to the number of contacts, participants were also asked about the type of the relationship (e.g., family, friends, neighbors), the type of contact (e.g., telephone, video conference and/or by written messages) and the emotional closeness (close, medium, low). We used an ego-centered “social network” circle to measure social contacts of older adults before, during and after the pandemic. The data collection was limited by the changing corona restrictions.ResultsResults indicate that behavior in social contacts essentially depends on age, gender, and level of depression. We found a clear temporal drop in social contacts independently of age and gender during the pandemic. After the pandemic close contacts did not recover to prepandemic level. Especially, Young-Old (&lt;72 years) recovered less in terms of the number of social contacts than the Old-Old (≥72 years).DiscussionOur study, thus, provides longitudinal insights into the course of social contacts and suggests that social isolation may have more negative and long-term impact on close contacts, which need further clarification and temporal extension.
HIV risk and influence factors among MSM who had sought sexual partners in core venues: a continuous sentinel surveillance in 2010–2022
Authors: Yang Zijie , Wei Lan , Xu Zhongliang , Li Simei , Xing Yiwen , Zhang Yan , Yuan Yuan , Liu Shaochu , Xie Wei , Tan Wei , Ye Wei , Tan Jingguang , Shi Xiangdong , Yan Xiangyu , Feng Tiejian , Jia Zhongwei , Zhao Jin 
Published: December 2024

BackgroundSeeking sexual partners in men who have sex with men (MSM) venues has been regarded as a high-risk behavior for HIV among MSM. Nevertheless, with the implementation of venue-based interventions and the change in the way MSM seek sexual partners, the continued status of MSM venues as the HIV risk factor remains inconclusive. This study endeavors to delve into this ambiguity by examining the MSM sexual contact network (SCN) as a foundation.MethodsA series of cross-sectional surveys were conducted in Shenzhen in the period 2010–2022. MSM sexual contact network and venue network were acquired, and network metrics were employed to identify core MSM and core venues. We compared the risk of HIV and risk behaviors between MSM who sought sexual partners in core venues and those who did not, with subgroup analyses based on different time periods.ResultsThe overall HIV prevalence among the 4,408 MSM surveyed in this study was 14.6%. Notably, 17 core venues were identified out of the 68 reported MSM venues, with 1,486 MSM who had sought sexual partners in core venues. These MSM had significantly higher risk of HIV and were more likely to take HIV testing and receive intervention services. Subgroup analyses showed that the heightened HIV risk associated with seeking partners in core venues was specific to the period 2010–2014, while HIV testing and service access remained consistently higher across all-period subgroups. Multiple sexual partners, seeking partners in core venues, receptive or both sexual roles, drug abuse, absence of HIV test, unprotected anal intercourse (UAI), and lower education levels were associated with elevated HIV risk among MSM.ConclusionFollowing the implementation of differentiated venue-based interventions, the risk of HIV among MSM who had sought sexual partners in core venues decreased to a level comparable to that of MSM who had not. The accessibility of HIV testing and intervention services remains uneven between MSM who had sought sexual partners in core venues and those who had not. As the Internet sex-seeking behavior gains prevalence among MSM, strategic adjustments of public health resource allocation may be necessary to address this imbalance.
Application of the transgender male voice questionnaire in a Brazilian population sample
Authors: Schwarz Karine , Villas-Bôas Anna Paula , Cielo Carla Aparecida , da Silva Dhiordan Carodoso , da Silva Eliane Dias , Fighera Tayane Muniz , Costa Angelo Brandelli , Lobato Maria Inês Rodrigues , Spritzer Poli Mara 
Published: December 2024

ObjectivesThe aim of this study was to adapt and apply the Portuguese version of the Transgender Man Voice Questionnaire in a sample of Brazilian transgender men and to investigate the relationship between voice satisfaction and hormone therapy duration. In addition, we suggest reducing and reformulating the questionnaire for screening.MethodsWe conducted a cross-sectional study of 31 transgender men aged 18–50 years undergoing hormone therapy who answered a questionnaire adapted from the Transgender Woman Voice Questionnaire, validated in Portuguese. Sociodemographic and clinical data were collected from the individuals’ electronic medical records: age, smoking status, and type and duration of hormone therapy. The questionnaire, consisting of 30 questions rated on a Likert scale, was answered individually during a psychotherapy session. In each question, the gender-specific words were modified. Furthermore, we added a question: 31 (After GAHT, my voice became completely male), with the response options yes or no. In questions 32 and 33, asking participants to provide an overall rating of their voice. Total score ranged from 0 to 120, with higher scores indicating greater dissatisfaction with voice.ResultsMean patient age was 30.13 ± 7.6 years, and 19.4% were smokers. The mean duration of hormone therapy was 29.7 ± 24.9 months, and 95% received intramuscular testosterone cypionate, maintaining serum testosterone levels within the male reference range. The questionnaire mean total score was 51 ± 17.72. There was a significant negative correlation between the questionnaire total score and duration of hormone therapy (r = −0.484, p = 0.006). The questionnaire had a high level of internal consistency/reliability, with a Cronbach’s alpha coefficient of 0.95 for all items and a split-half Spearman-Brown coefficient of 0.96. For the elaboration of a screening tool, it is suggested to remove questions 8, 10, 12, 13, 14, 17, 19, 23, 27, and 29 and modify question 1.ConclusionLonger hormone therapy favors voice deepening and satisfaction with voice. The psychometric properties of the Transgender Man Voice Questionnaire are reliable, supporting its use as a screening tool in clinical practice and as an adjunct to the planning of vocal and communication support for transgender individuals.
Multidimensional internet use related to cognitive performance in older persons: a nationwide cross-sectional study
Authors: Fan Yue , Wei Hua , Tao Qunshan 
Published: December 2024

IntroductionThe internet has been increasingly recognized as a potential driver for enhancing cognitive functioning in older adults; however, the mechanisms underlying this relationship remain insufficiently understood in the literature. This study aimed to investigate the associations between different dimensions of internet use—activity, device, and frequency—and cognitive performance in older adults.MethodsThis cross-sectional study utilized data from the fourth wave of the China Health and Retirement Longitudinal Study (CHARLS), involving a nationally representative sample of 17,676 participants aged 60 years and above. Multiple linear regression analyses were conducted to explore the relationship between MIU and cognitive ability. To explore the moderating effect of age on the relationship between internet use and cognitive functioning, the population was divided into upper (over 75 years) and lower (under 75 years) age groups.ResultsThe analysis revealed a significant positive association between internet use and cognitive function. Specifically, MIU were found to contribute positively to cognitive performance. Subgroup analyses showed that participants aged 60–75 years benefited more from internet use, whereas those over 75 years exhibited a weaker association, indicating a potential decline in the cognitive benefits with advancing age.ConclusionAccording to the results of the present study, MIU significantly increased the cognitive ability of older adult individuals. Additionally, MIU strongly influences components of cognitive functioning, including language, attention, calculation ability, orientation, memory. However, aging may weaken the relationship between MIU and cognitive ability.
The effects of dance interventions on reducing the risk of falls in older adults: a network meta-analysis
Authors: Li Ying , Wang Zhaoguo , Li Jiahao , Yang Honghao , Fang Zilong 
Published: December 2024

ObjectiveThe efficacy of dance in reducing fall risks among older adults highlights its potential for geriatric health, but the optimal dance style remains uncertain. The present study endeavors to systematically analyze the effects of various dance forms on reducing the risk of falls among older adult, with the aim of providing evidence-based insights into the most efficacious dance styles for this population.DesignThe network meta-analysis of the existing literature was conducted to synthesize the available evidence regarding the effectiveness of various dance interventions in reducing the risk of falls among older adults.MethodsWe searched seven databases for randomized controlled trials on dance interventions for fall prevention in older adults and used Stata 17.0 for network meta-analysis.ResultsTwenty-seven studies (n = 1,219 older adults) were included. Creative Dance (CD) significantly improved the Berg Balance Scale score in healthy older adults (p &lt; 0.05) and ranked best. Folk Dance (FD), CD, and Ballroom Dance (BD) all significantly improved Timed Up and Go time compared to controls, with FD ranking best overall. In Parkinson’s disease patients, both BD and FD significantly improved the Berg Balance Score, with FD again ranking best.ConclusionCreative dance, Folk dance, and Ballroom dance effectively reduce the risk of falls in older adults. Creative dance enhanced the Berg Balance Score the most among healthy older adults, while Folk dance improved the Timed Up and Go test performance the best. Similarly, among patients with Parkinson’s disease, Folk dance exhibits the best effect in improving Berg Balance Scale scores.Clinical trial registrationThe protocol of this study was registered with the International Prospective Register of Systematic Review, PROSPERO, under the identification number CRD42022323426, and can be integrally assessed online (https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42022323426).
Climate change impact on blood haemogram in the horse: a three-year preliminary study
Authors: Deniz Ömer , Aragona Francesca , Murphy Barbara A. , Tümer Kenan Çağrı , Bozacı Serkan , Fazio Francesco 
Published: December 2024

IntroductionThe global climatic changes pose a substantial threat to the well-being and productivity of both humans and animals.MethodsThis study examined the impact of climate changes during different seasons over a 3-year monitoring period (2021–2023) on various blood parameters including, white blood cells (WBC), neutrophils, basophils, eosinophils, lymphocytes, and monocytes, hematocrit (HCT), hemoglobin (HGB), red blood cells (RBC), platelets (PLT), mean corpuscular hemoglobin concentration (MCHC), mean corpuscular volume (MCV), and mean corpuscular hemoglobin (MCH). The study focused on 25 Thoroughbred mares located in Kastamonu-Türkiye. Thermal and hygrometric parameters, including ambient temperature, relative humidity, and ventilation, were collected. Subsequently, Temperature-Humidity index (THI) was computed. Blood samples were collected on the first day of every month from January 2021 to December 2023 and used for a complete blood count analysis. Between 2021 and 2023, changes in environmental indicators were correlated to changes in hematological parameters.ResultsTwo-way for repeated measures ANOVA revealed a significant seasonal fluctuation (&lt;0.0001) in ambient temperature, relative humidity, and THI. There was a reduction in RBC (&lt;0.01), and MCH (&lt;0.01) every year, HGB (&lt;0.0001) in summer 2021, 2022 and in summer and autumn 2023. HCT (&lt;0.0001), MCV (&lt;0.01), showed decreasing values in autumn 2022 and 2023. MCHC values showed increasing values in July and August 2021, 2022 and in June 2023. WBC levels increased throughout the spring periods of 2021 and 2022. In April 2021, there were elevated levels of lymphocytes and monocytes (&lt;0.0001) respectively.DiscussionThese findings could be helpful to promote the monitoring of physiological status both for the assessment of welfare status and for diagnostic purposes for the evaluation of possible disease outbreaks due to climate change in veterinary medicine.