bluuebunny/crossref_metadata_2025_split huggingface dataset:
The influence of the Municipal Human Development Index and maternal education on infant mortality: a parallel investigation in a retrospective cohort study in the extreme south of Brazil
Authors: Anele Carolina Ribeiro , Hirakata Vânia Naomi , Goldani Marcelo Zubaran , Silva Clécio Homrich da 
Published: September 2020

Abstract
Background

Infant mortality is considered an important and sensitive health indicator in several countries, especially in underdeveloped and developing countries. Most of the factors influencing infant mortality are interrelated and are the result of social issues. Therefore, this study performed a parallel investigation of the influence of the MHDI on the macro-region of residence and maternal education on infant mortality in a capital in the extreme south of Brazil.

Methods

It is a retrospective cohort study with data on births and deaths in the first year of live for the period of 2000-2017. The association between the independent variables and the outcome was done by bivariate analysis through simple Poisson regression. The variables that can potentially be considered confusing were used in a multiple Poisson regression for robust variances - adjusted model.

Results

The study included 311361 children, of whom 2271 died. Maternal education, individually and jointly analyzed with the MHDI, showed association with the outcome of infant death in the first year of life, particularly for children of mothers with lower maternal education (p<0.001). In relation to other related factors, maternal age; number of Prenatal Care Consultations; gestational age, weight, gender and Apgar Index (5th minute) of the newborn showed association with IM (p<0.001).

Conclusions

Although the HDI is considered a good predictor of infant mortality by some authors, the analyses of the present study did not present an association between the MHDI and death in the first year of life after adjustments for the other variables. Unlike the MHDI, maternal education under eight years of study maintained an association with infant death, proving to be a social determinant with relevant impact on infant mortality. Thus, it is concluded that maternal education, individually, is higher than the MHDI to assess the outcome of infant mortality and is easily accessible information.
Association between Short Sleep Duration and Overweight/Obesity among middle-school students: a cross-sectional study in Fuzhou, China
Authors: Li Guobo , Wei Shichao , Zheng Fuhao , Xu Xingyan , Cai Yingying , Wu Siying 
Published: September 2020

Abstract
Background: This study was designed to investigate the prevalence of short sleep duration and explore the association between short sleep duration and overweight or obesity in adolescents from middle school in Fuzhou, China. Methods: Questionaires focusing on short sleep duration and overweight or obesity related factors were collected. The sleep duration was self-reported by the subjects. The body weight and height of each subject were measured by the qualified personnel. Logistic regression analysis was used to evaluate the effects of short sleep duration on overweight or obesity in school students. Results: The overall rates of overweight and obesity were 12.1% and 7.1%, respectively. The prevalence of short sleep duration among students was 82.8%. The majority of high school students (92.5%) suffered from short sleep duration. Compared with male children with a sleep duration of over 8 hrs a day, the odds ratios (95% CI) of overweight/obesity for those with a sleep duration of less than 6 h or 6 - 8 h, were 1.63 (1.25-2.13) and 1.06 (0.88-1.27). After adjusting social and demographic status, mental health and lifestyle factors, the odds ratios of female children were 1.38(0.99-1.93) and 1.04 (0.79-1.35), respectively. Conclusions: A large number of adolescents suffered from short sleep duration. Short sleep duration was negatively correlated with overweight/obesity among male children.
Evolutionary engineering of Lactobacillus bulgaricus reduces enzyme usage and enhances conversion of lignocellulosics to D-lactic acid by simultaneous saccharification and fermentation
Authors: J. Vishnu Prasad , Sahoo Tridweep K. , S. Naveen , Jayaraman Guhan 
Published: September 2020

Abstract
BackgroundSimultaneous saccharification and fermentation (SSF) of pre-treated lignocellulosics to biofuels and other platform chemicals has long been a promising alternative to separate hydrolysis and fermentation processes. However, the disparity between the optimum conditions (temperature, pH) for fermentation and enzyme hydrolysis leads to execution of the SSF process at sub-optimal conditions, which can affect the rate of hydrolysis and cellulose conversion. The fermentation conditions could be synchronized with hydrolysis optima by carrying out the SSF at a higher temperature, but this would require a thermo-tolerant organism. Economically viable production of platform chemicals from lignocellulosic biomass (LCB) has long been stymied because of the significantly higher cost of hydrolytic enzymes. The major objective of this work is to develop an SSF strategy for D- lactic acid production by a thermo-tolerant organism, in which the enzyme loading could significantly be reduced without compromising on the overall conversion.ResultsA thermo-tolerant strain of Lactobacillus bulgaricus was developed by adaptive laboratory evolution (ALE) which enabled the SSF to be performed at 45 °C with reduced enzyme usage. Despite the reduction of enzyme loading from 15 Filter Paper Unit/ gLCB (FPU/gLCB) to 5 FPU/gLCB, we could still achieve ~8% higher cellulose to D-Lactic acid (D-LA) conversion in batch SSF, in comparison to the conversion by separate enzymatic hydrolysis and fermentation processes at 45 °C and pH 5.5. Extending the batch SSF to SSF with pulse-feeding of 5% pre-treated biomass and 5 FPU/gLCB, at 12-hour intervals (36th h – 96th h), resulted in a titer of 108 g/L D-LA and 60% conversion of cellulose to D-LA. This is one among the highest reported D-LA titers achieved from LCB.ConclusionsWe have demonstrated that the SSF strategy, in conjunction with evolutionary engineering, could drastically reduce enzyme requirement and be the way forward for economical production of platform chemicals from lignocellulosics. We have shown that fed-batch SSF processes, designed with multiple pulse-feedings of the pre-treated biomass and enzyme, can be an effective way of enhancing the product concentrations.
Sortilin levels correlate with major cardiovascular events of diabetic patients with peripheral artery disease following revascularization: a prospective study
Authors: Biscetti Federico , Nardella Elisabetta , Rando Maria Margherita , Cecchini Andrea Leonardo , Bonadia Nicola , Bruno Piergiorgio , Angelini Flavia , Stasi Carmine Di , Contegiacomo Andrea , Santoliquido Angelo , Pitocco Dario , Landolfi Raffaele , Flex Andrea 
Published: September 2020

Abstract
Background: Peripheral artery disease (PAD) represents one of the most relevant vascular complications of type 2 diabetes mellitus (T2DM). Moreover, T2DM patients suffering from PAD have an increased risk of major adverse cardiovascular events (MACE) and major adverse limb events (MALE). Sortilin, a protein involved in apolipoproteins trafficking, is associated with lower limb PAD in T2DM patients.Objective: To evaluate the relationship between baseline serum levels of sortilin, MACE and MALE occurrence after revascularization of T2DM patients with PAD and chronic limb-threatening ischemia (CLTI).Research Design and Methods: We performed a prospective non-randomized study including 230 statin-free T2DM patients with PAD and CLTI. Sortilin levels were measured before the endovascular intervention and incident outcomes were assessed during a 12-month follow-up.Results: Sortilin levels were significantly increased in individuals with more aggressive PAD (2.25 ± 0.51 ng/mL vs 1.44 ± 0.47 ng/mL, p < 0.001). During follow-up, 83 MACE and 116 MALE occurred. In patients, who then developed MACE and MALE, sortilin was higher. In particular, 2.46 ± 0.53 ng/mL vs 1.55 ± 0.42 ng/mL, p < 0.001 for MACE and 2.10 ± 0.54 ng/mL vs 1.65 ± 0.65 ng/mL, p < 0.001 for MALE. After adjusting for traditional atherosclerosis risk factors, the association between sortilin and vascular outcomes remained significant in a multivariate analysis. In our receiver operating characteristics (ROC) curve analysis using sortilin levels the prediction of MACE incidence improved (area under the curve [AUC] = 0.94) and MALE (AUC = 0.72).Conclusions: This study demonstrates that sortilin correlates with incidence of MACE and MALE after endovascular revascularization in a diabetic population with PAD and CLTI.
Baseline Well-being, Perceptions of Critical Incidents, and Openness to Debriefing in Community Hospital Emergency Department Clinical Staff Before COVID-19, a Cross-Sectional Study
Authors: Cantu Laura , Thomas Listy 
Published: September 2020

Abstract
Background: Emergency department personnel routinely bear witness to traumatic experiences and critical incidents that can affect their own well-being. Peer support through debriefing has demonstrated positive impacts on clinicians’ well-being following critical incidents. This study explored community hospital emergency department staff’s perceptions of critical incidents, assessed openness to debriefing and measured baseline well-being. Our analysis provides a baseline of provider well-being immediately prior to the local onset of COVID-19. The potential need for additional resources to support frontline providers during the pandemic can be evaluated. Method: We conducted a cross-sectional study for 4-weeks prior to the first COVID-19 case in Connecticut using a survey offered to an interprofessional group of emergency department clinical staff. The main outcome measures were the Hospital Anxiety and Depression Scale (HADS) and the Professional Quality of Life (ProQOL) scale. Pearson’s chi-square test was used to identify significant differences in perceptions of critical incidents and debriefings between professional categories. One-way ANOVA and Tukey’s test were used to analyze significant differences in well-being between professional categories. Results: Thirty-nine clinical personnel from St. Vincent’s Emergency Department responded to the survey. Events frequently selected as critical incidents were caring for critically ill children (89.7%), mass casualty events (84.6%), and death of a patient (69.2%). Critical incidents were commonly reported (81.6%) as occurring once per week. Additionally, 76.2% of participants reported wanting to discuss a critical incident with their team. Across all respondents, 45.7% scored borderline or abnormal for anxiety, 55.9% scored moderate for burnout, and 55.8% scored moderate to high for secondary traumatic stress.Conclusions: At baseline, providers reported caring for critically ill children, mass casualty events, and death of a patient as critical incidents, which typically occurred once per week. Death of a patient occurs at increased frequency during the protracted mass casualty experience of COVID-19 and threatens provider well-being. Receptiveness to post-event debriefing is high but the method is still underutilized. With nearly half of staff scoring borderline or abnormal for anxiety, burnout, and secondary traumatic stress at baseline, peer support measures should be implemented to protect frontline providers’ well-being during and after the pandemic.
Target screening of plant secondary metabolites in river waters by liquid chromatography coupled to high resolution mass spectrometry (LC-HRMS)
Authors: Nanusha Mulatu Yohannes , Krauss Martin , Schönsee Carina D. , Günthardt Barbara F. , Bucheli Thomas D. , Brack Werner 
Published: September 2020

Abstract
Background: Substantial efforts have been made to monitor potentially hazardous anthropogenic contaminants in surface waters while for plant secondary metabolites (PSMs) almost no data on occurrence in the water cycle are available. These metabolites enter river waters through various pathways such as leaching, surface run-off and rain sewers or input of litter from vegetation and might add to the biological activity of the chemical mixture. To reduce this data gap we conducted a LC-HRMS target screening in river waters from two different catchments for 150 plant metabolites which were selected from a larger database considering their expected abundance in the vegetation, their potential mobility, persistence and toxicity in the water cycle and commercial availability of standards. Results: The screening revealed the presence of 12 out of 150 possibly toxic PSMs including coumarins (bergapten, scopoletin, fraxidin, esculetin and psoralen), a flavonoid (formononetin) and alkaloids (lycorine and narciclasine). The compounds narciclasine and lycorine were detected at concentrations up to 3 µg/L while esculetin and fraxidin occurred at concentrations above 1 µg/L. Nine compounds occurred at concentrations above 0.1 µg/L, the Threshold for Toxicological Concern (TTC) for non-genotoxic and non-endocrine disrupting chemicals in drinking water. Conclusions: Our study provides an overview of potentially biologically active PSMs in surface waters and recommends their consideration in monitoring and risk assessment of water resources. This is currently hampered by a lack of effect data including toxicity to aquatic organisms, endocrine disruption and genotoxicity and demands for involvement of these compounds in biotesting.
Accuracy of Implant Surgical Guides Fabricated Using Computer Numerical Control Milling for Edentulous Jaws: A Pilot Clinical Trial
Authors: Chai Jinyou , Liu Xiaoqian , Schweyen Ramona , Setz Jürgen , Pan Shaoxia , Liu Jianzhang , Zhou Yongsheng 
Published: September 2020

Abstract
Background: To evaluate the accuracy of a computer numerical control (CNC) milled surgical guide for implant placement in edentulous jaws.Methods: Edentulous patients seeking implants treatment were recruited in this prospective cohort study. Radiographic guides with diagnostic templates were fabricated from wax-up dentures. Patients took cone-beam computed tomography (CBCT) wearing the radiopaque radiographic guides. Implant positions were virtually designed in the planning software based on the CBCT data, and the radiographic templates were converted into surgical guides using CNC milling technique. Forty-four implants were placed into 12 edentulous jaws following guided implant surgery protocol. Post-surgery CBCT scans were made for each jaw, and the deviations between the planned and actual implant positions were measured. Deviation of implant position was compared between maxilla and mandible, and between cases with and without anchor pins using independent t-test.Results: Nine patients (3 males and 6 females) with 12 edentulous jaws were recruited. The mean age of patients was 59.2±13.9 years old. All 44 implants was placed without complication and survived, The mean three dimensional linear deviation of implant position between virtual planning and actual placement was 1.53 ±0.48mm at the implant neck and 1.58 ±0.49 mm at the apex. The angular deviation was 3.96 ±3.05 degrees. No significant difference was found in the deviation of implant position between maxilla and mandible (P=0.28 at neck, 0.08 at apex), nor between cases with and without anchor pins (P=0.87 at neck, 0.06 at apex). Conclusions: The guides fabricated using the CNC milling technique provided comparable accuracy as those fabricated by Stereolithography. The displacement of the guides on edentulous arch might be the main contributing factor of deviation.Trial registration: Chinese Clinical Trial Registry, ChiCTR-ONC-17014159
Developing EU environmental standards for the food, drink and milk industries: key environmental issues and data collection
Authors: Santonja German Giner , Karlis Panagiotis 
Published: September 2020

Abstract
Background

The European Commission organised the review of the best available techniques reference document for the food, drink and milk industries according to the provisions of the Industrial Emissions Directive (Directive 2010/75/EU). Under this policy, an exchange of information between Member States, industrial organisations, non-governmental organisations promoting the protection of the environment and the European Commission took place.

Results

A crucial part for developing these EU environmental standards was a systematic determination of the key environmental issues of the food, drink and milk sector. Key environmental issues were defined for emissions to air and water, and for energy and water consumption. The key environmental issues were systematically determined by using four criteria proposed by the European Commission. Moreover, a data collection procedure was designed and real-plant emissions and consumption data were obtained for the first time from a representative variety of food, drink and milk installations across the European Union.

Conclusions

The development of the EU environmental standards for the food, drink and milk sector are based on a systematic determination of the key environmental issues and on a representative data collection for this sector. This paper also highlights the main normative, organisational and procedural factors addressed during the environmental policy integration carried out to develop these standards.
Frictional properties of anorthite (feldspar): Implications for the lower boundary of the seismogenic zone
Authors: Masuda Koji 
Published: September 2020

Abstract
Earthquake magnitude is closely related to the depth extent of the seismogenic zone, and higher magnitude earthquakes occur where the seismogenic zone is thicker. The frictional properties of the dominant mineral constituents of the crust, such as feldspar-group minerals, control the depth extent of the seismogenic zone. Here, the velocity dependence of the steady-state friction of anorthite, the calcic endmember of the feldspar mineral series, was measured at temperatures from 20 to 600 °C, pore pressures of 0 (“dry”) and 50 MPa (“wet”), and an effective pressure of 150 MPa. The results support previous findings that the frictional properties of feldspar play a dominant role in limiting the depth extent of the seismogenic zone. This evidence suggests that brittle deformation of anorthite may be responsible for brittle fault movements in the brittle–plastic transition zone.
Modeling Acute Ethanol-Induced Impairment with Gastric Organoids
Authors: Wang Wanjuan , Zhao Ying , Su Zeqi , Chu Fuhao , Li Tao , Li Yuan , Song Xiaoyu , Liu Xu , Liu Xing , Ding Xia 
Published: September 2020

Abstract
Background: Ethanol have been linked to atrophic gastritis and gastric carcinoma. Although it is well known that ethanol can result in hypochlorhydria, the molecular mechanisms underlying this phenomenon remain poorly understood.Results: Here we used gastric organoids to show that ethanol permeabilized the apical membrane of gastric parietal cells and induced ezrin hypochlorhydria. The functional consequences of ethanol on parietal cell physiology were studied using organoids. Gastric organoids were pre-incubated in the basic medium or with EGTA or E64 , and incubated at 37℃ in either medium alone, or medium containing 6% ethanol. We assessed ezrin proteolysis. Ethanol permeabilization induced activation of calpainⅠand subsequent proteolysis of ezrin, which resulted in the liberation of ezrin from the apical membrane of the parietal cells. Significantly, expression of calpain-resistant ezrin restored the functional activity of parietal cells in the presence of ethanol.Conclusion: Taken together, our data indicated that ethanol disrupted the apical membrane-cytoskeletal interactions in gastric parietal cells and thereby caused hypochlorhydria.
Pacemaker-generated stress fracture of the second rib: a case report
Authors: Ksela Jus , Racman Mark , Zbacnik Rok , Djordjevic Anze , Jan Matevz 
Published: September 2020

Abstract
Background: Pocket-related complications following the implantation of cardiovascular implantable electronic devices primarily include pocket hematoma, infection, skin erosion or decubitus, device migration, and Twiddler's syndrome, with other pathologies such as nerve impairment or bone lesions being extremely rarely encountered. We report a case of a 20-year old asthenic, non-athlete female patient presenting with a device-generated fracture of the second rib several months after sub-muscular permanent pacemaker implantation due to repeated bilateral pre-pectoral pocket infections.Case presentation: A 20-year old female patient was readmitted to our institution 9 months following sub-pectoral implantation of a permanent pacemaker, complaining of severe pocket-related pain, which arose spontaneously in the absence of direct trauma, intense physical activity or vigorous coughing, and was associated with normal day-to-day activity. To rule out a pacemaker re-infection, a native computed tomography and a positron emission tomography - computed tomography of the thorax were performed. Both modalities excluded an infection but showed a healing fracture and a focus of enhanced metabolic activity in the anterolateral part of the right second rib, indicating a non-traumatic or stress fracture of the bone. Consequently, a complete extraction of the pulse generator and both leads was performed and the smallest available single-chamber pulse generator with a single atrial electrode was implanted in the sub-fascial, pre-muscular pocket in the now recovered and uninfected left subclavicular region, alleviating patient’s severe pain symptoms and significantly enhancing her quality of life.Conclusions: In the absence of direct trauma, intense physical activity or vigorous coughing, we assume that in this asthenic girl a normal day-to-day motion of the right shoulder has persistently forced the sub-muscularly placed pulse generator toward thoracic wall, putting increased repetitive pressure force on the underlying bones, finally causing a fatigue stress fracture of the second rib. In asthenic phenotype patients with small thorax and short subclavicular distance, a sub-muscular pacemaker implantation can potentially cause unique and unexpected pocket-related adverse events necessitating advanced diagnostics and timely treatment.
NOD1 mediates interleukin-18 processing in epithelial cells
responding to Helicobacter pylori infection
Authors: Ferrero Richard , Tran Le Son , Ying Le , D'Costa Kimberley , Wray-McCann Georgie , Kerr Genevieve , Le Lena , Allison Cody , Ferrand Jonathan , Chaudhry Hassan , Emery Jack , De Paoli Amanda , Creed Sarah , Kaparakis-Liaskos Maria , Como Julia , Dowling Jennifer , Johanesen Priscilla , Kufer Thomas , Pedersen John , Mansell Ashley , Philpott Dana , Elgass Kirstin , Abud Helen , Nachbur Ueli , Croker Ben , Masters Seth 
Published: September 2020

Abstract
The interleukin-1 family members, IL-1β and IL-18, are processed into their biologically active forms by multi-protein complexes, known as inflammasomes. Although the inflammasome pathways that mediate IL-1β processing in myeloid cells have been well defined, those involved in IL-18 processing, particularly in non-myeloid cells, are still not well understood. Here, we report that the host defence molecule NOD1 regulates IL-18 processing in epithelial cells to the mucosal pathogens, Helicobacter pylori and Pseudomonas aeruginosa. We show that IL-18 is important in protecting against pre-neoplastic changes induced by gastric H. pylori infection in vivo. NOD1 mediates IL-18 processing via homotypic CARD-CARD interactions with caspase-1, and independently of canonical inflammasome proteins (NLRP3, ASC). These findings reveal an unanticipated role for NOD1 in the formation of bioactive IL-18, thereby underlining the differences in inflammasome functions between haematopoietic and non-haematopoietic cells.