bluuebunny/crossref_metadata_2025_split huggingface dataset:
Virtual Functional Mobility Test: A Potential Novel Tool for Assessing Mobility of Individuals With Parkinson´s Disease in a Multitask Condition.
Authors: Moura Júlia Araújo de , Leal Josevan Cerqueira , Piemonte Maria Elisa Pimentel , Quarles John P. , Mendes Felipe Augusto Dos Santos 
Published: December 2020

Abstract
Background: There are few instruments available for evaluating functional mobility during multitasking in people with Parkinson´s Disease. Virtual Reality is a potentially tool capable of aiding in the evaluation of functional mobility. The purpose of this study is to verify the potential of the Virtual Functional Mobility Test (VFMT) as a clinical tool to assess functional mobility of people with PD during multitasking condition. Method: 25 people with PD and 25 people without PD, matched for age and sex, were recruited. Participants were evaluated through the Trail Making Test, Timed "UP and GO" test, Timed “UG and GO” in dual task and through the VFMT, composed of 1) a simple task, involving exclusively motor skills and 2) a complex task, involving multitasking. The groups’ performances on the clinical and VFMT tests were compared to verify the virtual test´s sensitivity. It was evaluated the correlations between VFMT tasks and existing clinical tests, the reliability intra-rater and the users’ perception. Results: The VFMT and clinical tests were sensitive to differentiate the groups, except the trail making test part B (p= 0.332) and complex task (p= 0.052). Strong correlations were observed between parts A and B of the trail making test (r=0.75) and complex task (r=0.72); Moderate correlations between Timed Up and Go test and Timed Up and Go in dual task test with simple task (r=0.47) and complex task (r=0.55), respectively, was found. The complex task and simple task showed excellent and moderate reliability intra-rater, respectively. Conclusion: It was concluded that the novel VFMT is feasible, sensible, reliable and has potential as an instrument for the evaluation of functional mobility during multitasking in people with PD.
PCR-based Detection of Diarrheagenic Pathogens in Improved Water Sources: a Scoping Review Protocol of the Evidence in Low- and Middle-income Countries
Authors: Gemeda Shibabaw Tadesse , Soboksa Negasa Eshete , Tefera Yonatal Mesfin , Desta Adey Feleke , Gari Sirak Robele 
Published: December 2020

Abstract
Background: Occurrence of diverse human enteric bacterial, viral and protozoal pathogens in drinking water samples because of fecal contamination is of increasing public health concern, particularly in developing counties. Application of Polymerase Chain Reaction (PCR) based methods in detecting microbial quality of water provides more accurate, sensitive and rapid outcomes over conventional methods of microbial identification and quantification. Therefore, exploring water quality outcomes generated through PCR based methods is important to better understand the status and monitor progress towards internationally set goals for low- and middle-income countries. The aim of this scoping review is to map the existing evidence on the magnitude and characteristics of diarrheagenic pathogens as detected by PCR-based methods in improved water sources within the context of low- and middle-income countries. Methods: This scoping review will be undertaken in line with the JBI methodology for scoping reviews. We will consider studies from 2003 onwards that included PCR-based microbial water quality assessment of improved drinking water sources in low- and middle-income countries. The searches will be undertaken in PubMed/Medline, Scopus, Web of Science, JBI, Cochrane Library, and Google Scholar. A gray literature search will be conducted in Google and ProQuest. Discussion: This review will systematically discover and integrate the evidence available on the detection of diarrheagenic pathogens through the application of PCR based methods for water quality determination. In this review, information about the applied PCR method, detected diarrheagenic pathogens, water sample size, effectiveness in terms of time and cost, will be gathered and summarized. Diagrammatic and/or tabular presentation supported with a narrative synthesis will be used. The review will be reported according to a reporting guideline for scoping reviews: PRISMA extension for scoping reviews (PRISMA ScR).
Successful Transplantation of Spermatogonial Stem Cells Into the Seminiferous Tubules of Busulfan-treated Mice
Authors: Azizi Hossein , Tabar Amirreza Niazi , Skutella Thomas 
Published: December 2020

Abstract
Background: Spermatogonial stem cells (SSCs) in the testis are crucial for transferring genetic information to the next generation. Successful transplantation of SSCs to infertile men is an advanced therapeutic application in reproductive biology research. Methods: In this experimental research, both in vitro and in vivo characterization of undifferentiated and differentiated SSCs were performed by morphology - immunocytochemistry (ICC), immunohistochemistry (IMH), Fluidigm Real-Time polymerase chain reaction (RT-PCR) and flow cytometry analysis. The isolated SSCs were finally microinjected into the rete testis of busulfan-treated mice. The compact undifferentiated and more loosely connected round differentiated SSCs were isolated during testicular cell expansion from their specific feeder layer.Results: ICC analysis indicated high and low expression levels of Zbtb16 in undifferentiated and differentiated germ cells. Also, IMH analysis showed different expression levels of Zbtb16 in the two different germ stem cell populations of the testicular tissue. While Fluidigm RT-PCR analysis indicated overexpression of the TAF4B germ cell gene, the expression of DAZL, VASA, and Zbtb16 were down-regulated during the differentiation of SSCs (P< 0.05). Also, flow cytometry analysis confirmed the significant downregulation of Itgb1 and Itga4 during differentiation. By transplantation of SSCs into busulfan-treated NOD/SCID mice, GFP-labeled sperm cells developed. Conclusions: In the current study, we performed a transplantation technique that could be useful for the future microinjection of SSCs during infertility treatment and for studying in vivo differentiation of SSCs into sperm.
Warburg Effect-Promoted Exosomal Circ_0072083 Releasing Up-Regulates Nango Expression Through Multiple Pathways and Enhances Temozolomide Resistance in Glioma
Authors: Ding Chenyu , Yi Xuehan , Chen Xiangrong , Wu Zanyi , You Honghai , Chen Xiaoyong , Zhang Gaoqi , Sun Yong , Bu Xingyao , Wu Xiyue , Lin Zhangya , Gu Jianjun , Lin Yuanxiang , Kang Dezhi 
Published: December 2020

Abstract
BackgroundTemozolomide (TMZ) resistance limits its application in glioma. Exosome can carry circular RNAs (circRNAs) to regulate drug resistance via sponging microRNAs (miRNAs). miRNAs can control mRNA expression by regulate the interaction with 3’UTR and methylation. Nanog homeobox (NANOG) is an important biomarker for TMZ resistance. Hitherto, it is unknown about the role of exosomal hsa_circ_0072083 (circ_0072083) in TMZ resistance in glioma, and whether it is associated with NANOG via regulating miRNA sponge and methylation.MethodsTMZ-resistant (n=36) and sensitive (n=33) patients were recruited. The sensitive cells and constructed resistant cells were cultured and exposed to TMZ. circ_0072083, miR-1252-5p, AlkB homolog H5 (ALKBH5) and NANOG levels were examined via quantitative reverse transcription polymerase chain reaction and western blot. The half maximal inhibitory concentration (IC50) of TMZ, cell proliferation, apoptosis, migration and invasion were analyzed via Cell Counting Kit-8, colony formation, flow cytometry, wound healing and transwell assays. The in vivo function was assessed using xenograft model. The N6-methyladenosine (m6A) level was analyzed via methylated RNA immunoprecipitation (MeRIP). Target relationship was investigated via dual-luciferase reporter assay and RNA immunoprecipitation. Warburg effect was investigated via lactate production, glucose uptake and key enzymes expression. Exosome was isolated and confirmed via transmission electron microscopy and specific protein expression.Resultscirc_0072083 expression was increased in TMZ-resistant glioma tissues and cells. circ_0072083 knockdown restrained the resistance of resistant cells via decreasing IC50 of TMZ, proliferation, migration, invasion and xenograft tumor growth and increasing apoptosis. circ_0072083 silence reduced NANOG expression via blocking ALKBH5-mediated demethylation. circ_0072083 could regulate NANOG and ALKBH5 via targeting miR-1252-5p to control TMZ resistance. Warburg effect promoted the release of exosomal circ_0072083 in resistant cells. Exosomal circ_0072083 from resistant cells increased the resistance of sensitive cells to TMZ in vitro and xenograft model. Exosomal circ_0072083 level was enhanced in resistant patients, and it had a diagnostic value and indicated a lower overall survival in glioma. Conclusion﻿Exosomal circ_0072083 promoted TMZ resistance via increasing NANOG via regulating miR-1252-5p-mediated degradation and demethylation in glioma.
LINC00899 promotes osteogenic differentiation and alleviates osteoporosis via targeting miR-374a to regulate RUNX2
Authors: Gao Xiaoya , Xue Yun , Yang Kechun 
Published: December 2020

Abstract
Objective: This study aims to illustrate the underlying molecular mechanisms of long noncoding RNAs (LncRNAs) LINC00899 in osteoporosis.Methods: Real Time-quantitative Polymerase Chain Reaction (RT-qPCR) was used to examine the levels of LINC00899, miR-374a and RUNX2 in clinical tissues or human bone mesenchymal stem cells (hBMSCs). The interaction between miR-374a and LINC00899 or RUNX2 was predicted by starBase and verified by luciferase reporter assay and RNA binding protein immunoprecipitation (RIP) assay. Alkaline phosphatase (ALP) activity and Alizarin Red S (ARS) staining were also used to evaluate the osteogenic ability of hBMSCs.Results: The expression levels of LINC00899 were gradually increased, but miR-374a expression was decreased with the prolongation of osteogenic induction. In addition, the expression of LINC00899 was lowly expressed in osteoporotic patients’ bone tissues and knockdown of LINC00899 decreased the expression of osteogenesis-related genes. Moreover, LINC00899 was confirmed to inhibit miR-374a expression by direct interaction. Finally, we demonstrated that RUNX2 was a target of miR-374a, and the silencing of miR-374a partially abolished the inhibitory effect of LINC00899 knockdown on the expression of RUNX2, OPN and OCN.Conclusions: We demonstrated that LINC00899 facilitated the osteogenic differentiation of hBMSCs and prevented osteoporosis by sponging miR-374a and enhancing RUNX2 expression, which might provide a useful therapeutic strategy for osteoporosis patients.
Prevalence of Peripheral Neuropathic Pain among Adult Diabetic Patients (T2DM) with Peripheral Neuropathy and Quality of Life of the Patients with Peripheral Neuropathic Pain
Authors: Abu-Shennar Jawad Ahmad , Bayraktar Nurhan , Bebis Hatic 
Published: December 2020

Abstract
Aim The aim of the study was to assess the prevalence of diabetic peripheral neuropathic pain (DPNP) among adult patients suffering from type 2 diabetes mellitus (T2DM) with diabetic peripheral neuropathy (DPN) as well as to determine the quality of life of patients with DPNP.Materials and method The study designed in cross-sectional research model was conducted at the Jordanian Ministry of Health in Jordan, Amman during the period from 15 June 2019 to 5 October 2019. A total of 400 adult T2DM patients with DNP were recruited for the study. Data were collected by way of an assessment tool package, including descriptive data (demographics, anthropometric measurements, laboratory measurements, and clinical data), the Douleur Neuropathique4 (DN4) questionnaire, physical exam, Quality of Life Questionnaire (EQ-5D), and Numeric Pain Rating Scale (NRS). To analyze study data descriptive and inferential statistics were used.Results Results of the current study showed that the prevalence of DPNP among adult T2DM patients with DPN was high (47.8%). About half of the participants had mild or moderate pain with the most frequently reported symptoms of tingling and burning. Uncontrolled diabetes was found to be the main predictor of DPNP among patients with DPN. Participants who had DPNP reported having problems in connection with mobility, self-care, routine activities, discomfort, and anxiety or/and depression, and they had a significantly lower quality of life with poorer self-rated health status than those without DPNP. Besides, the effect of pain on their quality of life was found to be significant.Conclusion The study recommends, in accordance with the results, that strategies should be developed for an effective management of painful DPN with an integrated and interdisciplinary approach. Current clinical guideline recommendations should be followed to improve patient care and reduce the burden of the disease.
Evaluation of S2 alar and traditional S1 pedicle fixation for severe lumbar spondylolisthesis in different bone mineral densities: a finite element analysis
Authors: Wang Juehan , Chen Wei , Yang Xi , Zhu Ce , Ling Tingxian , Feng Ganjun , Song Yueming , Liu Limin 
Published: December 2020

Abstract
Purpose: Little is known about the biomechanical performance of L5-S2 alar internal fixation constructs after posterior lumbar interbody fusion. This study aimed to compare the biomechanical effect of L5-S1 internal fixation and L5-S2 alar internal fixation on severe lumbar spondylolisthesis. Methods: A normal male volunteer without a history of spinal disease was selected, lumbar CT data was collected. An intact L5-S2 three-dimensional finite element model was created by Mimics and Geomagic.Two kinds of fixation methods were reconstructed including (1) the L5-S1 screw fixation model and (2) the L5-S2 alar fixation model. The inverse repair was performed using Geomagic software, the internal fixation device was drawn using Creo software, and the model parameters were set and analyzed using ANSYS Workbench software. Results: The validity of the intact model shows that the ROM of the model is similar to that of a reported cadaveric study. The average stress of the L5-S2 alar internal fixation device was 86.9-111% higher(P＜0.001) than that of the L5-S1 fixation device when the bone of the S1 screw path reached the yield threshold. The maximal stress of the S1 screw in the L5-S2 alar fixation was significantly lower (P＜0.001) than it in the L5-S1 fixation when the stress exceeds the S1 bone yield threshold. When the S1 screw path bone yielded, the maximal deformation value of the S1 screw path was similar in both models (P>0.05), while the average deformation value of the S1 screw path in L5-S2 alar fixation was significantly higher (P＜0.01) than it in L5-S1 fixation. Conclusion: Extending fixation to the S2 wing can significantly improve internal fixation device stability and reduce the risk of intraoperative and postoperative fractures while avoiding injury to the sacroiliac joint, reducing the difficulty of surgery and the risk of injury to surrounding tissues. It is a reasonable plan for the treatment of moderate and severe lumbar spondylolisthesis with osteoporosis.
Dietary Intakes and Nutritional Status Among Migrant Children in Primary Schools: A Cross-Sectional Study
Authors: Zeng Jing , Shao Lijing , Li Yongjun , Yang Mei , Xiang Bing , Hao Liping , Yang Xuefeng , Cheng Guangwen 
Published: December 2020

Abstract
Background: Nutrition plays a crucial role in children’s growth and development, migrant children have their own characteristics in nutrition. The current study aims to assess the dietary status of migrant school-age children and underlying factors. Methods: A cross-sectional survey was carried out on migrant children in 25 classes with the 3rd to 6th grades from two primary schools by cluster sampling. Children’s 3-day 24-hour diet were recorded, the dietary intake of calories and nutrients were calculated by using the China Food Composition for each day, and were presented the average intake of the 3 day period. The assessment of energy and nutrient intakes by estimated energy requirement (EER) and estimated average requirement (EAR), respectively. The Dietary Guidelines for Chinese Residents and Chinese Dietary Reference Intakes were used as evaluation standards to assess the dietary intakes and nutritional status of these children.Results: Participants included 752 migrant children aged 9-12y (430 boys and 322 girls). Deficiency proportions of energy and protein were 46.1% and 40.1% for boys, 44.4% and 65.1% for girls. Energy supply proportions of macronutrients (protein, fat, and carbohydrate) in boys were 12.4%、28.8%、58.8%, those of girls were 11.1%、28.6%、60.3%. The insufficiency proportions of vitamin A, B vitamins, calcium, zinc and selenium were considerably serious (>60%). In dietary pattern, intakes of cereal, poultry and edible oil were relatively adequate, but milk, fish, eggs and soybean products were seriously inadequate. Conclusions: Characteristics such as unreasonable dietary pattern, relative lack of energy and nutrients, and excess snack intake coexist, it is necessary to carry out nutrition intervention to help migrant children to balance the diet and rectify the deficiencies.
PENGEMBANGAN MODEL PEMBELAJARAN BERBASIS ‎TEKNOLOGI INFORMASI PADA PESANTREN DI ‎SUMATERA BARAT
Authors: Rajab Rajab , Zulmuqim Zulmuqim , Hidayatullah Rully 
Published: December 2020

Abstrak: Penelitian ini bertujuan untuk mengembangkan proses pembelajaran berbasis teknoligi informasi di pesantren, medorong pendidik/guru di pesantren menggunakan teknologi informasi dalam proses pembelajaran. Metode yang digunakan dalam penelitian ini adalah metode deskriptif dan dipadukan dengan kajian perpustakaan, sehingga akan mendapatkan konsep dan kajian yang mampu membawa pesantren ke era revolusi industeri 4.0. Permasalahan yang terjadi di pesantren antara lain proses pembelajaran di kelas masih terpusat pada guru, sumber pengetahuan utama masih berasal dari guru dan literasi yang berkembang masih calistung (membaca, menulis dan berhitung). Hasil penelitian antara lain; pimpinan pesantren dapat melaksanakan pembelajaran berbasis teknologi informasi mengunakan Learning Management System (LMS), seperti; Google Classroom, Zoom Clouds Meeting, Schoology dan LMS lainnya. Sehingga proses pembelajaran tidak hanya terpusat pada guru tetapi terpusat pada peserta didik/santri dan literasi yang digunakan adalah literasi data, literasi manusia dan literasi teknologi.
Kata Kunci: Revolusi Industri 4.0, pembelajaran, teknologi informasi.
KOMUNIKASI PROFETIK RASULULLAH DALAM MEMBANGUN UKHUWWAH DI MADINAH
Authors: Mukoyimah Mukoyimah 
Published: December 2020

<p>Communication is a core activity in every interaction. Every interaction contains a vision, so does the Prophet Muhammad. as a communicator. Prophetic communication of the Prophet needs to be an example/<em>uswatun hasanah</em> in building <em>ukhuwwah</em>. This study aims to provide an understanding back to the communicator/<em>da'i</em> of the current era about what prophetic communication should be done in facing of the complex conditions in the middle of the communicant. This research is a qualitative library research on hadiths delivered by the Prophet in Medina. While the data collection techniques is documentation and the data analysis used is interpretation. The results of this study shows the prophetic communication built on the personal and communal understanding of communicant, giving rise to the right method and way to build <em>ukhuwwah</em> in Medina. The communication of <em>Rasulullah</em> was carried out clearly, repeating the message of <em>da'wah</em>, and adjusting the capability of the <em>mad'u</em>/communicant faced so that the Islamic community's <em>ukhuwwah</em> was well developed.</p><p> </p>
PELAYANAN KOMUNIKASI PERBANKAN DAN KEPUASAN NASABAH (PERSPEKTIF KOMUNIKASI ISLAM)
Authors: Perdana Dian Adi 
Published: December 2020

<p><em>This article explains about the optimization of banking communication services that causes customer (campus community) dissatisfaction. Banking communication services as the main source of the problem are not being able to use internet banking in banking services, the lack of employees at the nearest BTN operational office, payment schedules that are often delayed because of internet integrity connecting between the campus and the bank is broken and service dissatisfaction has not yet reached the remote area. This study is a qualitative research by doing interview, observation, and documentation in data collection. The interactive analysis model is done in a descriptive qualitative manner and the data is presented with a SWOT analysis to determine the company's situation and strategy. The conclusion is the component of customer satisfaction in the form of: responsiveness, compliance, empathy, tangible, reliability and assurance which are elements of patterns of improvement in communication services have a positive influence on customer satisfaction in using banking services. This must be started and addressed by prioritizing customer needs. Researchers found that service influences customer satisfaction, which means that the greater the influence of service, the greater the satisfaction achieved by these customers, and these efforts continue to be carried out by BTN in maintaining customer trust in the company. Another thing is the need to optimize the strength they have to respond to opportunities that arise from external companies.</em></p><p> </p>
HOAKS DAN ALQURAN: UPAYA MEMBANGUN PERADABAN KRITIS DALAM MENERIMA BERITA DAN ADAB DALAM BERKOMUNIKASI
Authors: Wirdiyana Salwa Sofia 
Published: December 2020

<p><em>This paper seeks to parse the solution to the problem of hoax from the perspective of Quranic interpretation holistically and comprehensively. This study is a qualitative research by reading the relationship (munasabah) between verses about hoax in the Holy Quran that are represented by the terms ifk, fa&gt;siq, muna&gt;fiq, murjifu&gt;n, and tabayyun. After reading the verses related to the term, it was concluded that hoax news can be minimized by critical thinking, having emotional maturity, performing tabayyun, and broadening insight. In addition, the Holy Quran also teaches good communication ethics, namely qaulan sadi&gt;dan (correct speech), qaulan bali&gt;gan (good words that leave an imprint on the soul), qaulan maisu&gt;ran (proper speech), qaulan layyinan (soft words), qaulan kari&gt;man (noble words), and qaulan ma'ru&gt; fan (good words).</em></p><p> </p>
Prośrodowiskowe kształtowanie terenów zieleni - propozycje dla dzielnicy Ursynów m.st. Warszawy
Authors: Staroszczyk Justyna , Skowroński Antoni 
Published: December 2020

It is well known that green areas are a significant component of urban environment. Plants influence urban conditions in many ways; they have a positive effect on both people’s health and environmental issues, there are many reasons why the proportion of green areas in cities should be increased. It is important to take advantage of plant properties and suitable methods of shaping green areas to benefit nature.
The article emphasizes the importance of comprehensive environmental protection. It presents selected methods of shaping green areas and nursing plants, which are in common use in Western Europe and USA, but are under-used in Poland, the main aim of the article is to propose solutions which allow to shape green areas in the Ursynów district of Warsaw in favor of environmental protection e.g. in a way that makes it possible to preserve biodiversity or reduce drink water use.