bluuebunny/crossref_metadata_2025_split huggingface dataset:
Binalebu-3: A high yielding, year-round, scented and seedless variety of lemon
Authors: Alam M S , Islam M R 
Published: February 2025

An investigation was carried out in three consecutive years at the Horticulture Division, Bangladesh Institute of Nuclear Agriculture (BINA), Mymensingh to characterize lemon germplasm for varietal development. Thirty-one local and exotic lemon germplasm were collected from home and abroad. The germplasm CL-0021 and CL-0027 were collected from Darziling, India. These two germplasm alone with check variety were evaluated during 2017-18, 2018-19 and 2019-20 at two location viz.Kashiar char, Mymensingh and Garobazar, Madhupur, Tangail. The experiments were carried out following randomized complete block design with three replications. BAUlebu-4 was used as a check variety in the experiments. From the results it was observed that CL-0021 was found to be superior to other exotic genotype and check variety BAUlebu-4 in case of fruit setting, disease and disease resistant. During 2017-18, 2018-19 & 2019-20 at Mymensingh, the highest fruit yield plant-1(55.50, 42.23 kg and 33.01 kg respectively), vitamin C (88.15, 88.21and 88.14 mg 100 g-1), plant height (225.55 cm, 189.11 cm and 165.01 cm), fruit length (7.92, 7.71 cm and 7.69 cm), number of fruits plant-1 (250.00, 2014.24 and 150.00), juice content fruit-1 (48.00%, 46.22% and 44.74%) and the lowest number of seeds fruit-1 (4.2,4.1, and 4) was recorded from CL-0021 in all the three consecutive year trials. Similar results were also observed at another location, Garobazar, Madhupur, which was higher compared to CL-0027 and BAUlebu-4 at both locations. The lowest fruit yield plant-1, vitamin C, the highest number of seeds fruit-1 observed at CL-0027 at both of Mymensingh and Tangail. Considering the better performance of fruit yield, vitamin C content, juice content and less number of seeds compare to CL-0021, CL-0027 and BAUlebu-4; CL-0021 genotype was selected as a new variety naming Binalebu-3 to grow commercially all over Bangladesh.
Bangladesh J. Nuclear Agric, 38(2): 27-34, 2024
Adoption and profitability of Binadhan-16 in some selected areas of Bangladesh
Authors: Sultana R , Rahman R H , Sarkar M M A , Haque M R , Islam S 
Published: February 2025

The study was conducted in three Binadhan-16 growing areas of Bangladesh, namely Mymensingh, Netrokona and Rangpur districts. The objectives of the study were to find out the determinants of adoption of Binadhan-16 rice variety at the farm level and to estimate the costs and return of Binadhan-16 cultivation in the study areas. Moreover, some problems of the Binadhan-16 cultivation were also investigated in this study. This study was based on primary data which were collected from 180 farmers, 60 from each district where 30 was adopter and 30 was non-adopter of Binadhan-16 growers. Data were collected through face to face interview with the help of a pre-designed interview schedule from three upazillas i e. Valuka (Mymensingh), Purbadhala (Netrokona) and Sadar (Rangpur). Descriptive statistics and logit regression model were used to fulfill the objectives. The result of logit regression model shows that farm size, yield, seed, training and extension contact have positive and significant effect on Binadhan-16 adoption. Education and cultivation duration were found as negatively significant variables in explaining the variation in Binadhan-16 adoption. The study also found that total variable cost of Binadhan-16 cultivation was Tk. 44346 per hectare which was 66.78% of total cost of production. The highest cost item was human labour which accounted for 46.44 % of the total cost. Cost of land preparation was 17.84% and land use cost accounted for 14.63% of total cost and ranked as second and third cost items, respectively. On an average, the total cost of production was Tk. 66404 per hectare where 33.22% was fixed costs and 66.78% was variable cost. It was found that Binadhan-16 production is profitable. The highest yield was found at Rangpur (5546 kg ha-1) followed by Mymensingh (5379 kg ha-1) and Netrokona (5297 kg ha-1). The average selling price of Binadhan-16 was Tk. 24.88 kg-1 where cost of production was Tk. 12.28 kg-1. The average gross margin was found Tk. 98756 on variable cost basis. The average net return per hectare was Tk. 76698. The average Benefit Cost Ratio (BCR) was 2.16. Regarding major problems of the variety cultivation, 15% mentioned about high labour cost which ranked top followed by quality seed in timely manner (14%), infestation of disease (11%), scarcity of labour (9%), low price of paddy and high transportation cost (8%). As the variety yield higher profit more emphasis should be given on farm size, seed, yield, and training programme for the expansion of the variety.
Bangladesh J. Nuclear Agric, 38(2): 125-135, 2024
Assessing mutation accumulation in DNA repair-deficient Listeria monocytogenes: implications for cgMLST cluster thresholds in outbreak analysis
Authors: Füszl Astrid , Pietzka Ariane , Hyden Patrick , Mösenbacher Tobias , Stöger Anna , Blaschitz Marion , Stadlbauer Silke , Hasenberger Petra , Schindler Stefanie , Heger Florian , Pleininger Sonja , Indra Alexander 
Published: February 2025

BackgroundListeria (L.) monocytogenes is primarily transmitted via contaminated food and can cause listeriosis, an infection often associated with sepsis and meningitis in at-risk individuals. Accurate outbreak detection relies on whole genome sequencing (WGS) and core genome multilocus sequence typing (cgMLST), which use allele thresholds to identify related strains.MethodsThis study investigated mutation rates in L. monocytogenes, focusing on isolates with DNA repair deficiencies. Serial subcultivations were performed, comparing a repair-deficient isolate with a wild-type control. Genetic variability was assessed using WGS and cgMLST.ResultsMutation rates were significantly higher in repair-deficient isolates, exceeding typical cgMLST thresholds currently used in Listeria outbreak investigations, leading to a misclassification of related isolates as unrelated. An additional analysis of the Austrian Listeria database revealed that such deficiencies are rare among isolates.ConclusionsThe standard 7-allele cgMLST threshold effectively identifies related strains in most cases, but may require adjustments for hypermutator strains. Incorporating DNA repair data could improve the accuracy of outbreak investigations, ensuring reliable public health responses.
A systematic review on evaluating school safety signs and ways to improve its effectiveness
Authors: Wang Tongxu , Abdul Shukor Shureen Faris , Kozlowski Marek , Wan Mohamed Wan Srihani , Zhang Ting 
Published: February 2025

IntroductionSafety signs are essential visual communication tools that convey critical information regarding hazards, regulations, and emergency procedures across various environments. Their significance has garnered considerable attention due to their role in enhancing public safety. However, existing studies have predominantly focused on design factors, with limited research addressing the interaction of non-design factors among specific age groups and safety communication in public spaces, particularly concerning young children’s cognitive abilities and perceptions.MethodsThis systematic review aims to comprehensively analyze the characteristics and effectiveness of safety signs within the school environment. A systematic review of the literature on safety signs—specifically their design within school premises—was conducted using the Systematic Review and Meta-Analyses (PRISMA) approach to identify research gaps. Query strings were utilized to extract papers, identifying 48 peer-reviewed articles from 2000 to 2024 for review.ResultsThe review found that visual elements, such as dynamic safety signs and the use of highly saturated colors, significantly enhance the effectiveness of warnings. Several factors were identified that influence the efficacy of safety signs, including age-related cognitive abilities and the contextual understanding of young children. Additionally, the study highlights the effectiveness of technological tools, such as eye tracking and virtual reality (VR), for assessing human perception and improving testing methodologies. The role of enjoyment and experiential learning in enhancing safety communication are beneficial.DiscussionThis systematic review underscores the importance of considering both design and non-design factors in safety sign development, particularly in school environments. The findings suggest that dynamic, visually appealing signs, along with the use of advanced technological tools, can improve the effectiveness of safety messages. The integration of experiential learning also contributes to a better understanding of safety information. This review is a valuable resource for safety science researchers, visual designers, and policymakers by providing insights into the current state of research, identifying research gaps, and offering perspectives on the future of school safety signage pertinent to school-age children.
Case report: A novel intronic JMJD6 likely pathogenic variant (c.941+75G > T) associated with congenital eyelid coloboma in one of the identical twin sisters
Authors: Li Xin , Zhang Yuqi , Chai Gang , Su Weijie , Zhang Yan 
Published: February 2025

BackgroundCongenital eyelid coloboma (CEC) is a rare genetic disease, manifesting as a congenital partial or total defect of the eyelid. In this study, we report a pedigree with CEC caused by a novel pathogenic variant in JMJD6.Case reportThe proband was a 3-year-old girl who presented with a congenital coloboma of the left upper eyelid, accompanied by hypoplasia of the ipsilateral eyebrow. Karyotype analysis was normal. Whole-exome sequencing (WES) identified a novel pathogenic variant in JMJD6 (c.941+75G &gt; T), which was classified as a likely pathogenic (LP) and de novo variant. To date, this variant has not been reported.ConclusionOur study found a novel pathogenic variant in JMJD6 (c.941+75G &gt; T), which broadens the CEC phenotype spectrum and JMJD6 gene variant spectrum, providing a basis for clinical diagnosis, genetic counseling, and treatment.
Prevalence of stress and associated factors among students in Ethiopia: a systematic review and meta-analysis
Authors: Bezie Anmut Endalkachew , Abere Giziew , Zewude Girum Tareke , Desye Belay , Daba Chala , Abeje Eyob Tilahun , Keleb Awoke 
Published: February 2025

IntroductionStress is an increasing public health issue among the student population. This stress affects their academic performance, mental health, and overall well-being. As a result, we conducted a systematic review and meta-analysis to determine the pooled prevalence and associated factors of stress among students in Ethiopia.MethodsAn extensive search of electronic databases such as PubMed, Google Scholar, Semantic Scholar, HINARI, and Science Direct, cross-referencing, and Google manual search was conducted to identify cross-sectional studies published from 1998 to 2024. The preferred PRISMA guideline was used to report items for this systematic review and meta-analysis. To extract data, Microsoft Excel 16 and to analyze STATA 17 software were used. The JBI quality assessment tool was used for the included studies with medium to high-quality scores. To estimate the pooled prevalence of stress and its associated factors, a random effects model was used. The funnel plot and Egger’s regression test were used to assess publication bias, and I2 test statistics were used to determine heterogeneity of the included studies. The protocol for this review has been registered with PROSPERO (ID: CRD42024578490).ResultsA total of 23 studies with 8,946 study participants met the inclusion criteria. The pooled prevalence of stress among students in Ethiopia was 37.64% (95% CI: 29.61–45.66; I2 = 98.8%). Female gender (OR = 1.82, 95% CI: 1.57–2.12), rural resident (OR = 1.51, 95% CI: 1.22–1.87), living outside university dormitory (OR = 2.02, 95% CI: 1.34–3.05), the habit of alcohol consumption (OR = 1.46, 95% CI: 1.12–1.91), being a cigarette smoker (OR = 2.36, 95% CI: 1.49–3.74), being a khat chewer (OR = 1.35, 95% CI: 1.02–1.80), working in an unfavorable environment (OR = 1.80, 95% CI: 1.20–2.71), and having poor social support (OR = 1.93, 95% CI: 1.39–2.68), were significantly associated with an increased risk of stress.ConclusionThe findings of this systematic review and meta-analysis indicated a high prevalence of stress among students. The study identified female gender, being a rural residence, living outside a university dormitory, khat chewing, having the habit of alcohol consumption, working in an unfavorable environment, poor social support, and cigarette smoking as significant risk factors for stress. To develop coping skills and resilience, integrating mental health education into the curriculum could help students to prevent stress. Furthermore, strategies such as academic support programs, substance use reduction programs, counseling services, and stress management workshops could be beneficial.Systematic review registrationhttps://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42024578490, CRD42024578490.
Hybrid Feel-Own-Move®: protocol for an effectiveness-implementation study of a psychomotor intervention for survivors of domestic violence
Authors: Machorrinho Joana , Veiga Guida , Marmeleira José , Scheffers Mia , Santos Graça Duarte 
Published: February 2025

BackgroundDomestic violence is a public health concern, impacting the health and well-being of women and children globally. Shelter homes are one of the support services for victims’ recovery, although providing holistic healthcare in this setting remains a struggle. Feel-Own-Move® (FOM) is an evidence-based psychomotor intervention designed to help women who have experienced domestic violence reconnect with their bodies. Hybrid FOM (H-FOM) is a version of FOM that combines in-person with online sessions for both women and children living in shelter homes. To examine the effectiveness and implementation success of H-FOM are the aims of this study.MethodsThis protocol details an effectiveness-implementation type I hybrid study, to be carried out in shelter homes across three European countries. Health outcomes of the participants, and the implementation success within professionals from the shelter homes and the psychomotor therapists responsible for implementing H-FOM will be assessed. Results will be analyzed through a mixed methods approach, following the conceptual model of implementation science and the RE-AIM framework.DiscussionThis effectiveness-implementation study is expected to contribute to understanding H-FOM health-related effects on women and children survivors of violence, as well as to its sustainable implementation, up-scaling and integration into trauma support services and associated healthcare policy. H-FOM is expected to (i) improve the known effects of FOM on women survivors of DV, while ensuring continuity of the therapeutic process following relocation, and promoting the health and well-being of children living in the shelter homes.
The impact of resource management practices on the performance of micro and small enterprises in Werabe Town, Ethiopia
Authors: Refera Matewos Kebede , Habib Redela Seman , Lemma Habtamu Regassa , Seman Abdilkerim Asrar , Antonios Endalew Adamu 
Published: February 2025

IntroductionEfficient resource management is essential for enhancing organizational performance; however, its implementation in micro and small enterprises (MSEs) has received limited academic attention. Taking into account these obvious reasons, this study aims to investigate the influence of resource management practices on the performance of micro and small enterprises in Werabe Town, Ethiopia.MethodsWe distributed a structured questionnaire to 265 randomly selected small business operators. Both descriptive and inferential statistical methods, including correlation and regression analysis were used to analyse the collected data.ResultsThe findings indicate low overall MSE performance, driven by inadequate financial, human resource, material, and information management practices. Based on regression analysis, these four factors explain 64.3% of the variations in performance, showing that they have a positive and statistically significant effect.ConclusionTherefore, improving financial planning, cash flow, and working capital management is crucial for maintaining efficient financial operations. However, financial efficiency alone is not enough; a formal HR planning process, a well-defined job description, carrying out a recruiting and selection, and establishing a pay system are equally crucial. Additionally, streamlining material requirement planning, procurement processes, and inventory control systems ensures resource availability and operational efficiency. Furthermore, pre-made and streamlined information management software is appropriate to improve the flow of information for better decision-making across financial, human, and material resources.
Kami Fumi-e: Japanese Paper Images to Be Trampled on—A Mystery Resolved
Authors: Montanari Riccardo , Colomban Philippe , Alberghina Maria Francesca , Schiavone Salvatore , Pelosi Claudia 
Published: February 2025

There has been long-standing debate as to whether Kami Fumi-e (paper images to be trampled on) had actually been used in image trampling sessions as part of the 250-year persecution of Christianity enforced by the Tokugawa Shogunate. Sacred images of Christianity officially recorded to have been trampled on are housed in the permanent collection of the Tokyo National Museum and are almost uniquely made of metal alloy. The controversy regarding paper images, apart from the medium being considered unsuitable for such extreme use, was fueled by the appearance of a significant number of them in museum collections and institutions worldwide in the 20th century. Most of the prints bear dates from different eras of the Edo period, sometimes hundreds of years apart; therefore, long-standing arguments regarding their authenticity marked the last century. In order to distinguish later copies from potentially original pieces, if ever existed, XRF, Raman, and FTIR analytical techniques were used to study the materials characterizing them. In addition, detailed observation of the main visual features (overall design composition, inscriptions, paper support, etc.) was carried out to highlight potential discrepancies that could pair with scientific evidence and lead to a definitive conclusion.
Arabidopsis thaliana Plants’ Overexpression of the MYB Transcription Factor VhMYB60 in the Face of Stress Hazards Enhances Salt and Cold Tolerance
Authors: Chen Zhe , Wang Jinghan , Li Wenhui , Chen Xiang , Zhao Changjia , Guo Yanbo , Li Yingnan , Chen Zhuo , Li Xingguo , Han Deguo 
Published: February 2025

‘Beta’ (Vitisriparia × V. labrusca) is a vine fruit tree of the genus Vitis which is a cross between American and riparian grapes. In the current situation of grape production in northern regions, cold, drought, and salinity are important bottlenecks restricting its development, while some grape rootstocks with excellent traits show the disadvantage of poor resilience. ‘Beta’ (Vitis riparia × V. labrusca), one of the most extensively utilized rootstocks in viticulture, has demonstrated remarkable resilience to adverse conditions. However, the mechanisms by which ‘Beta’ rootstocks resist abiotic stresses are unknown and need to be further investigated. In this study, we successfully isolated and cloned a novel MYB transcription factor, VhMYB60, from the ‘Beta’ grapevine. This factor spans 972 base pairs and encodes a protein comprising 323 amino acids. Subcellular localization studies revealed that VhMYB60 is predominantly expressed within the nucleus. Furthermore, tissue-specific expression analysis demonstrated that VhMYB60 is more abundantly expressed in the mature leaves and roots of the grape plant. Further studies showed that salt and cold stress notably increased VhMYB60 gene expression in both mature leaves and grape roots. Compared with the control, Arabidopsis thaliana (Arabidopsis) plants molecularly modified to overexpress VhMYB60 exhibited enhanced salt and cold resistance and improved survival rates. Moreover, notable changes were detected in chlorophyll, malondialdehyde (MDA), proline, peroxidase (POD), catalase (CAT), and superoxide dismutase (SOD) levels. Concurrently, the expression levels of structural genes that are positively correlated with resistance to adversity stress were markedly elevated in Arabidopsis plants that overexpress VhMYB60. Consequently, VhMYB60 may serve as a pivotal transcription factor in the regulation of ‘Beta’ resistance.
SsNEP2 Plays a Role in the Interaction Between Sclerotinia sclerotiorum and Coniothyrium minitans
Authors: Zhao Huizhang , Zhu Zihang , Xu Yueli , Wang Haixuan , Xie Jiatao , Cheng Jiasen , Jiang Daohong , Fu Yanping 
Published: February 2025

Sclerotinia sclerotiorum, a fungal pathogen that is spread worldwide and causes serious diseases on crops, can be parasitized specifically by the mycoparasite Coniothyrium minitans. SsNEP2, encoding a necrosis-inducing protein in S. sclerotiorum, was previously inferred to play a role in the virulence to host plants. In this study, silencing of SsNEP2 in S. sclerotiorum had no significant (p < 0.01) influence on mycelial morphology, while overexpression led to lower mycelial growth and more branches. When amended with the fermentation broth of the SsNEP2 silencing mutants, conidial germination of C. minitans was promoted, while conidial production decreased. When parasitized by C. minitans, enhanced resistance of the SsNEP2 silencing mutants and weaker resistance of the overexpressed transformants were observed compared to the wild-type S. sclerotiorum strain 1980. In addition, the expression of SsNEP2 in C. minitans enhanced mycelial parasitism on S. sclerotiorum and restored the effect of silencing SsNEP2 in S. sclerotiorum on mycoparasitism. Thus, we highlight the role of SsNEP2 as a PAMP-like protein in the mycoparasitism between C. minitans and its host fungus S. sclerotiorum. SsNEP2 can be used to promote the biological potential of C. minitans.
Metabolomic Profiling of the Striatum in Shank3 Knockout ASD Rats: Effects of Early Swimming Regulation
Authors: Meng Yunchen , Hu Yiling , Xue Yaqi , Zhen Zhiping 
Published: February 2025

Objectives: This study aimed to investigate the regulatory impact of early swimming intervention on striatal metabolism in Shank3 gene knockout ASD model rats. Methods: Shank3 gene knockout exon 11–21 male 8-day-old SD rats were used as experimental subjects and randomly divided into the following three groups: a Shank3 knockout control group (KC), a wild-type control group (WC) from the same litter, and a Shank3 knockout swimming group (KS). The rats in the exercise group received early swimming intervention for 8 weeks starting at 8 days old. LC-MS metabolism was employed to detect the changes in metabolites in the striatum. Results: There were 17 differential metabolites (14 down-regulated) between the KC and WC groups, 19 differential metabolites (18 up-regulated) between the KS and KC groups, and 22 differential metabolites (18 up-regulated) between the KS and WC groups. Conclusions: The metabolism of striatum in Shank3 knockout ASD model rats is disrupted, involving metabolites related to synaptic morphology, and the Glu and GABAergic synapses are abnormal. Early swimming intervention regulated the striatal metabolome group of the ASD model rats, with differential metabolites primarily related to nerve development, synaptic membrane structure, and synaptic signal transduction.