bluuebunny/crossref_metadata_2025_split huggingface dataset:
Circ6401, a novel circular RNA, is implicated in repair of the damaged endometrium by Wharton’s jelly-derived mesenchymal stem cells through regulation of the miR-29b-1-5p/RAP1B axis
Authors: Shi Qin , Sun Baolan , Wang Di , Zhu Yi , Zhao Xinxin , Yang Xiaoqing , zhang yuquan 
Published: August 2020

Abstract
Background Accumulating evidence indicates that mesenchymal stem cells (MSCs) exert tissue repair effects and therapeutic angiogenesis through their noncoding RNAs (ncRNAs). Our previous studies showed that MSCs derived from Wharton’s jelly (WJ-MSCs) can ameliorate damaged human endometrium by promoting angiogenesis. There is limited information on the functions and mechanism of ncRNAs in MSC-induced endometrial repair, and additional studies are needed for more insights.Methods Here, WJ-MSCs were cocultured with or without endometrial stromal cells (ESCs) damaged by mifepristone (cocultured group versus non-cocultured group). TUNEL staining assays, EdU proliferation assays, flow cytometry apoptosis assays, and western blot assays were performed to observe the reparative effect of WJ-MSCs on damaged ESCs. Subsequently, circular RNA (circRNA) and microRNA microarrays were performed between the two groups. A subset of top upregulated circRNAs was validated by qRT-PCR. The functions of circ6401 (hsa_circ_0006401) in WJ-MSCs were investigated using lentivirus-mediated circRNA overexpression assays. The subcellular localization of circ6401 and miR-29b-1-5p in WJ-MSCs was identified by double RNA fluorescence in situ hybridization. Dual-luciferase reporter assays and western blot assays were performed to elucidate the regulatory mechanisms among circ6401, miR-29b-1-5p, and RAP1B.Results WJ-MSCs significantly improved ESC proliferation and upregulated the expression of vascular angiogenesis markers. Circ6401 was upregulated in WJ-MSCs cocultured with damaged ESCs, while miR-29b-1-5p was significantly downregulated. Furthermore, circ6401 was found to bind to miR-29b-1-5p and prevent it from decreasing the level of RAP1B, a crucial protein involved in the VEGF signaling pathway, which promoted angiogenesis and stimulated the proliferation of ESCs.Conclusions Our results showed the abundance and regulation profiles of ncRNAs of WJ-MSCs during repair of damaged ESCs, and for the first time, clarified the underlying mechanism by which circ6401 promotes endometrial repair by WJ-MSCs; thus, demonstrating that circ6401 may serve as a potential therapeutic target.
Relationship between alcohol consumption and cancer in rural Chinese adults: 1 5- year follow-up cohort study
Authors: Zhang Yue , Li Jingyi , Cheng Nannan , Yang Jie , Ye Lijing , Lu Xiaofan , Zhou Ziyi , Wang Zhuo , Liu Lishun , Song Yun , Li Chuan , Lu Qian , Wang Gailing , Huang Xiao , Tang Genfu , Wang Binyan , Qin Xianhui , Zalloua Pierre , Zhang Huisheng , Yan Fangrong , Xu Xiping 
Published: August 2020

Abstract
Background：We aimed to evaluate the association between alcohol consumption and risk of cancer incidence among rural Chinese adults. Methods: We utilized data from a community-based cohort study in rural China enrolled in 2003 and followed up prospectively up to 2018. Generalized estimating equation models were used to obtain odds ratios (OR) and 95% confidence intervals (CI) to analyze the relationship between alcohol consumption and cancer incidence. Results: After an average of 15 years of follow-up, a total of 9870 adult participants were included in this study. The results of the regression analysis for males showed that former drinkers had a significantly increased risk of cancer compared to never drinkers ([OR]2.46,95%[CI](1.43-4.23)). The cancer risk for current drinkers with heavy alcohol consumption(>400g/week) significantly increased ([OR]1.66,95% [CI] (1.18-2.34))compared to never drinkers. Among current drinkers, for every 100g of alcohol consumed per week, the risk of cancer increased by 15%. Among current drinkers, those aged 53.5 years or older , had a significant increase in the risk of cancer ([OR]1.26,95% [CI](1.12-1.42), for those with triglycerides ≥150 mg/dL, the risk of cancer was even higher ([OR]1.50,95%[CI](1.20-1.88), P for interaction 0.018), and for those with high density lipoprotein cholesterol (HDLC)<40 mg/dL, the risk of cancer increased the greatest ([OR]2.03,95%[CI](1.36-3.04), P for interaction 0.005). Conclusions: Among middle-aged and elderly males in rural China, the risk of cancer significantly increased among former and heavy current drinkers compared with never drinkers. Age, triglycerides, and HDLC may increase the risk of cancer along with alcohol consumption.
Ovarian Cancer Cells Direct Monocyte Differentiation Through a Non-canonical Pathway
Authors: Fogg Kaitlin C. , Miller Andrew E. , Li Ying , Flanigan Will , Walker Alyssa , O’Shea Andrea , Kendziorski Christina , Kreeger Pamela 
Published: August 2020

Abstract
Background: Alternatively-activated macrophages (AAMs), an anti-inflammatory macrophage subpopulation, have been implicated in the progression of high grade serous ovarian carcinoma (HGSOC). Increased levels of AAMs are correlated with poor HGSOC survival rates, and AAMs increase the attachment and spread of HGSOC cells in vitro. However, the mechanism by which monocytes in the HGSOC tumor microenvironment are differentiated and polarized to AAMs remains unknown. Methods: Using an in vitro co-culture device, we cultured naïve, primary human monocytes with a panel of five HGSOC cell lines over the course of seven days. An empirical Bayesian statistical method, EBSeq, was used to couple RNA-seq with observed monocyte-derived cell phenotype to explore which HGSOC-derived soluble factors supported differentiation to CD68+ macrophages and subsequent polarization towards CD163+ AAMs. Pathways of interest were interrogated using small molecule inhibitors, neutralizing antibodies, and CRISPR knockout cell lines. Results: HGSOC cell lines displayed a wide range of abilities to generate AAMs from naïve monocytes. Much of this variation appeared to result from differential ability to generate CD68+ macrophages, as most CD68+ cells were also CD163+. Differences in tumor cell potential to generate macrophages was not due to a MCSF-dependent mechanism, nor variance in established pro-AAM factors. TGFa was implicated as a potential signaling molecule produced by tumor cells that could induce macrophage differentiation, which was validated using a CRISPR knockout of TGFA in the OVCAR5 cell line. Conclusions: HGSOC production of TGFa drives monocytes to differentiate into macrophages, representing a central arm of the mechanism by which AAMs are generated in the tumor microenvironment.
The predictive value of preoperative positive urine cytology for development of bladder cancer after nephroureterectomy in patients with upper urinary tract urothelial carcinoma: A retrospective multi-center case-control study and meta-analysis
Authors: Fan Bo , Huang Yuanbin , Wen Shuang , Sun Min , Yang Xinrui , Sun Man , Chen Yuting , Huang Yan , Wang Yumei 
Published: August 2020

Abstract
Background: Upper urinary tract urothelial carcinoma (UUT-UC) is a rare and severe urinary malignancy. Urine cytology is a common clinical method for the early diagnosis of urologic neoplasms. Several studies have explored the relationship between preoperative urine cytology and intravesical recurrence in patients with UUT-UC. However, the results of these studies are controversial or even contradictory, and investigations with UUT-UC patients in Northeast China are rare. Methods: In this retrospective case-control study, we first estimated the prognostic significance of preoperative urine cytology in the outcomes of intravesical recurrence in 142 patients with UUT-UC after radical nephroureterectomy (RNU). A nomogram model was established and we evaluated its effectiveness. Subsequently, after searching several databases, including PubMed, Embase, and Ovid, we quantitatively combined our results with the published data in a meta-analysis. Results: Our case-control study demonstrated that patients with muscle-invasive tumors (T2-T4) were more prone to preoperative positive urine cytology than those with non-muscle-invasive tumors (Tis-T1), suggesting that preoperative positive urine cytology may be associated with the aggressive form of UUT-UC. Kaplan-Meier curves demonstrated that the patients with positive urine cytology had significantly poorer intravesical recurrence-free survival (IV-RFS). Additionally, preoperative urine cytology (hazard ratio, HR=3.24) and lymph node status (HR=2.67) were validated as significant independent predictors of intravesical recurrence by multivariate analysis. Nomogram, calibration plots, AUC values and the C-index demonstrated that the predictive accuracy was significantly improved when preoperative urine cytology was combined. Subsequently, adding to our study, 11 eligible articles from 2010 to 2016 containing 2942 patients were sifted out for our meta-analysis. Overall analysis showed that preoperative positive urine cytology was associated with a 56% increased risk of intravesical recurrence (HR=1.56). In the subgroup analysis by region, study type, and sample size, the pooled HR was statistically significant for the Japan subgroup (HR=1.45), China subgroup (HR=1.66), cohort study subgroup (HR=1.44), and case-control study subgroup (HR=1.77), the subgroup with sample size greater than or equal to 100 (HR=1.42), and the subgroup with sample size less than 100 (HR=2.95). Conclusions: Preoperative urine cytology significantly correlated with intravesical recurrence in patients with UUT-UC after RNU, although these results need to be interpreted with caution. Large, prospective trials are required to further confirm its prognostic significance.
Dysregulation of ferroptosis may involve in the development of non-small cell lung cancer in Xuanwei area
Authors: Yang Zhang , Li Guangjian , Yang Jiapeng , Zhao Guangqiang , Shen Zhenghai , Yang Kaiyun , Tian Linwei , Zhou Qinghua , Peng Ren-Wang , Chen Ying 
Published: August 2020

Abstract
Background

The Xuanwei area of Yunnan Province, China is one of the regions with the highest incidence of lung cancer in the world. Local residents use bituminous coal as fuel for cooking and heating, which causes serious indoor air pollution. After the local government carried out furnace and stove reform work, the high incidence of lung cancer in residents continued. We herein wonder if there are specific mechanisms at protein level for the development of lung cancer in the area.
Methods

We investigated the changes of protein profiling in tumor of the patients from Xuanwei area. Tandem Mass Tag (TMT) were employed to screen the differential proteins between carcinoma and para-carcinoma tissues.
Results

We identified a total of 422 differentially-expressed proteins, among which 162 proteins were significantly upregulated and 260 were downregulated compared to para-carcinoma tissues. Many of the differentially-expressed proteins were related to ECM-receptor interaction, focal adhesion, PI3K/AKT pathway and ferroptosis. Further experiments on the two differential proteins, TXN2 and HP, showed that the change of their expressions could make the lung cancer cell lines more resistant to erastin or RSL-induced ferroptosis in vitro, and promote the growth of tumor in nude mice.
Conclusion

This study revealed that aberrant regulation of ferroptosis may involve in the development of lung cancer in Xuanwei area.
Social Perspective of Female Genital Mutilation/Cutting in Khartoum State – Sudan 2020
Authors: Elnimeiri Mustafa Khidir Mustafa , Satti Shahenaz seifalseen Mustafa , Abdelrahim Taqwa Mohieldeen Hamid , Ibrahim Mohanad Kamaleldin Mahmoud , Abdelbasit Reem Mahmoud Mohamed , Mingaryous Dimetry Adel Dimetry 
Published: August 2020

Abstract
Background Female genital cutting( FGC) comprises all procedures that involve partial or total removal of the external female genitalia, or other injury to the female genital organs for non-medical reasons and it is considered as violation of human rights of girls and women. The overall objective of this study was to explore the social factors contributing in the persistence of this practice despite of the efforts for eradication in Khartoum State-Methods: This was a community based cross-sectional study conducted in Khartoum State- Sudan including the three localities. The study included 920 females within the reproductive age, sampled proportionate to size using multistage cluster sampling The data were collected using standardized administered questionnaire, Data were analyzed using statistical package for social science's version 21. Analysis was composed of descriptive data and Multinominal Regression Test to study the associations between variables of interest.Results: 62% of the participants considered female genital cutting as a violation of girls’ and women’s rights, 20% of them mentioned rejection by husbands as the main risk if daughters were not subjected to female genital cutting ,71% of the participants considered female genital cutting as a harmful traditional practice while 26% did not consider it as such . ] Multinomial logistic regression estimates of daughters have been subjected to any form of female genital cutting and reasons of conduct of female genital cutting and the risk encountered when girls are not subjected to female genital cutting showed ,“the risk encountered when girls are not subjected to female genital cutting” ranked the first place in influence as the regression coefficient of this variable is 68.779, while the main reason for the conduct of female genital cutting ranked the second place in influence as the regression coefficient of this variable is .089.Conclusions: FGC is a cultural practice recognized as a violation of human rights. These findings support the social coordination norm model, and results indicate the widely prevailing misconceptions about FGC/M among the study participants especially among those participants supporting the continuation of FGC/M. Key words Female genital cutting, Social Factors, Violation, Sudan
Left ventricular fractional shortening as a novel predictor of clinical outcomes in patients with coronary artery disease after undergoing PCI: A Retrospective Cohort Study
Authors: Guo Qian-Qian , Tang Jun-Nan , Yang Xu-Ming , Zhang Jian-Chao , Cheng Meng-Die , Song Feng-Hua , Liu Zhi-Yu , Wang Kai , Jiang Li-Zhu , Fan Lei , Yue Xiao-Ting , Bai Yan , Dai Xin-Ya , Zhang Zeng-Lei , Zheng Ru-Jie , Zheng Ying-Ying , Zhang Jin-Ying 
Published: August 2020

Abstract
Background: Even though great advances have been made in the treatment of coronary artery disease (CAD) owing to coronary revascularization and modern antiremodeling therapy, it remains the major cause of cardiac morbidity and mortality worldwide. Risk stratification in CAD patients is primarily based on left ventricular ejection fraction (LVEF), risk scores, and some serum markers. The value of baseline Left ventricular fractional shortening (LVFS) level in predicting the clinical outcomes has not yet been determined.Methods: In this retrospective cohort study, a total of 3561 patients were enrolled in Clinical Outcomes and Risk Factors of Patients with CAD after percutaneous coronary intervention (PCI), from January 2013 to December 2017. After excluding patients without echocardiography data, we finally enrolled 2787 patients. These patients were divided into two groups according to LVFS value. The lower group (LVFS <31%, n=741), the higher group (LVFS≥31%, n=2046). The average follow-up time were 37.59±22.24 months.Results: We found that there were significant differences between the two groups in the incidence of all-cause mortality (ACM) (P<0.001), cardiac mortality (CM) (P<0.001), major adverse cardiovascular events (MACEs) (P<0.05) and major adverse cardiovascular and cerebrovascular events (MACCEs) (P<0.05). Multivariate Cox regression analyses showed that LVFS was an independent predictor for ACM (hazard ratio [HR]:0.473 [95% confidence interval [CI]:0.290-0.772],P=0.003), CM (HR: 0.393 [95% CI:0.213-0.725],P=0.003) in acute coronary syndrome (ACS) patients but that it was an independent predictor for only the incidence of CM (HR: 0.153 [95% CI:0.046-0.504],P=0.002) in stable CAD patients.Conclusion This study indicates that baseline LVFS is an independent and novel predictor of adverse long-term outcomes in CAD patients who underwent PCI.
Analysis Using Random Forest and Data Integration Methods of Non-promoter Methylation and Expression Data Identifies Dysregulated Genes in Central Carbon Metabolism Pathway in Oral Cancer
Authors: Majumder Partha P. , Mukhopadhyay Srija , Ghosh Sahana , Das Debodipta , Arun P. , Roy Bidyut , Biswas Nidhan K. , Maitra Arindam 
Published: August 2020

Abstract
Background: Studies of epigenomic alterations associated with diseases primarily focus on methylation profiles of promoter regions of genes, but not of other genomic regions. In our past work (Das et al. 2019) on patients suffering from gingivo-buccal oral cancer – the most prevalent form of cancer among males in India – we have also focused on promoter methylation changes and resultant impact on transcription profiles. Here, we have investigated alterations in non-promoter (gene-body) methylation profiles and have carried out an integrative analysis of gene-body methylation and transcriptomic data of oral cancer patients. Methods: Tumor and adjacent normal tissue samples were collected from 40 patients. Data on methylation in the non-promoter (gene-body) regions of genes and transcriptome profiles were generated and analyzed. Because of high dimensionality and highly correlated nature of these data, we have used Random Forest (RF) and other data-analytical methods.Results: Our integrative analysis of non-promoter methylation and transcriptome data has revealed significant methylation-driven alterations in some genes that also significantly impact on their transcription levels. These changes result in enrichment of the Central Carbon Metabolism (CCM) pathway, primarily by dysregulation (overexpression) of (a) NTRK3, which plays a dual role as an oncogene and a tumor suppressor; (b) SLC7A5 (LAT1) which is a transporter dedicated to essential amino acids, and is overexpressed in cancer cells to meet the increased demand for nutrients that include glucose and essential amino acids; and, (c) EGFR which has been earlier implicated in progression, recurrence, and stemness of oral cancer, but we provide evidence of epigenetic impact on overexpression of this gene for the first time. Conclusions: In rapidly dividing cancer cells, metabolic reprogramming from normal cells takes place to enable enhanced proliferation. In the present analysis, we have identified that among oral cancer patients, genes in the CCM pathway – that plays a fundamental role in metabolic reprogramming – are significantly dysregulated because of perturbation of methylation in non-promoter regions of the genome. This result compliments our previous result that perturbation of promoter methylation results in significant changes in key genes that regulate the feedback process of DNA methylation for the maintenance of normal cell division.
Eating alone at each meal and associated health status by cohabitation situation among Japanese community-dwelling elderly: a cross-sectional analysis of the KAGUYA study
Authors: Kushida Osamu , Moon Jong-Seong , Matsumoto Daisuke , Yamasaki Naomi , Takatori Katsuhiko 
Published: August 2020

Abstract
Background: This study investigated the association between eating alone at each meal and health status including functional capacity according to cohabitation situation among Japanese community-dwelling elderly.Methods: This was a cross-sectional analysis of baseline data from the Keeping Active across Generations Uniting the Youth and the Aged (KAGUYA) study in Japan. A self-administered questionnaire was mailed to all 8004 residents aged 65 or older residing in the same Japanese town the participants in March 2016. Eating alone was assessed by first asking whether participants ate three separate meals each day (i.e., breakfast, lunch, and dinner), and those who answered affirmatively were then asked how many people were usually present at each meal. Health status was assessed in terms of subjective health, medical history, care needs, body mass index, depression, and functional capacity.Results: Data from 3057 respondents were analyzed. Among those living with others, those who reported not being in good subjective health and a history of hypertension were significantly more likely to eat alone at breakfast (odds ratio 1.27; 95% confidence interval 1.01–1.61, and 1.26; 1.06–1.49). Depressive symptoms and many subscales of functional capacity were also significantly associated with eating alone at breakfast, lunch, and dinner (P < 0.05). Among those living alone, those eating alone at breakfast had lower scores for indicators of functional capacity including information practice (P = 0.010) and total scores (P = 0.049).Conclusions: For both cohabitation situations, many health status indicators were related to eating alone at each meal, especially breakfast.
Genetic background contributions to the disproportion of vitamin A deficiency in pregnant women of certain ethnicities in populations of the United States.
Authors: Suzuki Masako , Wang Tao , Garretto Diana J , Isasi Carmen R. , Cardoso Wellington V , Greally John M. , Quadro Loredana 
Published: August 2020

Abstract
Vitamin A is an essential micronutrient that plays critical roles in many biological functions of the body. Limited access to vitamin A-rich food or supplements severely affects tissue and blood levels of vitamin A. Therefore, low serum vitamin A and poverty levels are strongly associated in vitamin A deficiency (VAD) studies that have focused mainly on developing countries. The current national prevalence rate of vitamin A deficiency in the United States is reported to be very low (< 1%). However, several studies, including ours, have suggested that people from certain ethnic groups still face a higher proportion of vitamin A deficiency. Here, we re-analyzed two different datasets of serum retinol levels of pregnant females to assess the VAD status differences between women of different ancestries. We found that pregnant females with non-Hispanic Black and with Latin American/Afro-Caribbean ancestry have strikingly high proportions of vitamin A deficiency. Moreover, we identified candidate genetic variants that associate with the disproportions between these different ancestries. Maternal vitamin A deficiency increases the risk of adverse health outcomes for both the mother and offspring later in life. Measuring serum retinol levels of pregnant women in the higher risk groups and provision of food interventions based on genetic information to improve the vitamin A status of at-risk women are needed. Our study strongly suggests that emergency actions need to be taken to reduce vitamin A deficiency in specific, at-risk ethnic groups.