bluuebunny/crossref_metadata_2025_split huggingface dataset:
External Validation of the DCD-N Score and a Linear Prediction Model to Identify Potential Candidates for Organ Donation After Circulatory Death: A Nationwide Multicenter Cohort Study
Authors: Nijhoff Maaike F. , Pol Robert A. , Volbeda Meint , Kotsopoulos Angela M.M. , Sonneveld Johan P.C. , Otterspoor Luuk , Abdo Wilson F. , Silderhuis Vera M. , El Moumni Mostafa , Moers Cyril 
Published: August 2020

Background.
Donation after circulatory death (DCD) is a procedure in which after planned withdrawal of life-sustaining treatment (WLST), the dying process is monitored. A DCD procedure can only be continued if the potential organ donor dies shortly after WLST. This study performed an external validation of 2 existing prediction models to identify potentially DCD candidates, using one of the largest cohorts.

Methods.
This multicenter retrospective study analyzed all patients eligible for DCD donation from 2010 to 2015. The first model (DCD-N score) assigned points for absence of neurological reflexes and oxygenation index. The second model, a linear prediction model (LPDCD), yielded the probability of death within 60 min. This study determined discrimination (c-statistic) and calibration (Hosmer and Lemeshow test) for both models.

Results.
This study included 394 patients, 283 (72%) died within 60 min after WLST. The DCD-N score had a c-statistic of 0.77 (95% confidence intervals, 0.71-0.83) and the LPDCD model 0.75 (95% confidence intervals, 0.68-0.81). Calibration of the LPDCD 60-min model proved to be poor (Hosmer and Lemeshow test, P < 0.001).

Conclusions.
The DCD-N score and the LPDCD model showed good discrimination but poor calibration for predicting the probability of death within 60 min. Construction of a new prediction model on a large data set is needed to obtain better calibration.
Meta-azotomics of engineered wastewater treatment processes reveals differential contributions of established and novel models of N-cycling
Authors: Park Mee-Rye , Annavajhala Medini K. , Chandran Kartik 
Published: August 2020

AbstractThe application of metagenomics and metatranscriptomics to field-scale engineered biological nitrogen removal (BNR) processes revealed a complex N-cycle network (the meta-azotome) therein in terms of microbial structure,potentialandextantfunction. Autotrophic nitrification bore the imprint of well-documentedNitrosomonasandNitrospirain most systems. However, in select BNR processes, complete ammonia oxidizing bacteria, comammoxNitrospira, unexpectedly contributed more substantially to ammonia oxidation than canonical ammonia oxidizing bacteria, based on metatranscriptomic profiling. Methylotrophic denitrification was distinctly active in methanol-fed reactors but not in glycerol-fed reactors. Interestingly, glycerol metabolism and N-reduction transcript signatures were uncoupled, possibly suggesting the role of other carbon sources in denitrification emanating from glycerol itself or from upstream process reactors. In sum, the meta-azotome of engineered BNR processes revealed both traditional and novel mechanisms of N-cycling. Similar interrogation approaches could potentially inform better design and optimization of wastewater treatment and engineered bioprocesses in general.
Measuring the density and viscosity of culture media for optimized computational fluid dynamics analysis ofin vitrodevices
Authors: Poon Christine 
Published: August 2020

AbstractCulture medium is frequently modelled as water in computational fluid dynamics (CFD) analysis ofin vitroculture systems involving flow, such as bioreactors and organ-on-chips. However, culture medium can be expected to have different properties to water due to its higher solute content. Furthermore, cellular activities such as metabolism and secretion of ECM proteins alter the composition of culture medium and therefore its properties during culture. As these properties directly determine the hydromechanical stimuli exerted on cellsin vitro, these, along with any changes during culture must be known for CFD modelling accuracy and meaningful interpretation of cellular responses. In this study, the density and dynamic viscosity of DMEM and RPMI-1640 media supplemented with typical concentrations of foetal bovine serum (0, 5, 10 and 20% v/v) were measured to serve as a reference for computational design analysis. Any changes in the properties of medium during culture were also investigated with NCI-H460 and HN6 cell lines. The density and dynamic viscosity of the media increased proportional to the % volume of added foetal bovine serum (FBS). Importantly, the viscosity of 5% FBS-supplemented RPMI-1640 was found to increase significantly after 3 days of culture of NCI-H460 and HN6 cell lines, with distinct differences between magnitude of change for each cell line. Finally, these experimentally-derived values were applied in CFD analysis of a simple microfluidic device, which demonstrated clear differences in maximum wall shear stress and pressure between fluid models. Overall, these results highlight the importance of characterizing model-specific properties for CFD design analysis of cell culture systems.
Curvilinear features are important for animate/inanimate categorization in macaques
Authors: Yetter Marissa , Robert Sophia , Mammarella Grace , Richmond Barry , Eldridge Mark A. G. , Ungerleider Leslie G. , Yue Xiaomin 
Published: August 2020

AbstractThe current experiment investigated the extent to which perceptual categorization of animacy, i.e. the ability to discriminate animate and inanimate objects, is facilitated by image-based features that distinguish the two object categories. We show that, with nominal training, naïve macaques could classify a trial-unique set of 1000 novel images with high accuracy. To test whether image-based features that naturally differ between animate and inanimate objects, such as curvilinear and rectilinear information, contribute to the monkeys’ accuracy, we created synthetic images using an algorithm that distorted the global shape of the original animate/inanimate images while maintaining their intermediate features (Portilla and Simoncelli, 2000). Performance on the synthesized images was significantly above chance and was predicted by the amount of curvilinear information in the images. Our results demonstrate that, without training, macaques can use an intermediate image feature, curvilinearity, to facilitate their categorization of animate and inanimate objects.
Gene dosage screens in yeast reveal core signalling pathways controlling heat adaptation
Authors: Jann Cosimo , Johansson Andreas , Smith Justin D. , Parts Leopold , Steinmetz Lars M. 
Published: August 2020

AbstractHeat stress causes proteins to unfold and lose their function, jeopardizing essential cellular processes. To protect against heat and proteotoxic stress, cells mount a dedicated stress-protective programme, the so-called heat shock response (HSR). Our understanding of the mechanisms that regulate the HSR and their contributions to heat resistance and growth is incomplete. Here we employ CRISPRi/a to down- or upregulate protein kinases and transcription factors inS. cerevisiae. We measure gene functions by quantifying perturbation effects on HSR activity, thermotolerance, and cellular fitness at 23, 30 and 38°C. The integration of these phenotypes allowed us to identify core signalling pathways of heat adaptation and reveal novel functions for the high osmolarity glycerol, unfolded protein response and protein kinase A pathways in adjusting both thermotolerance and chaperone expression. We further provide evidence for unknown cross-talk of the HSR with the cell cycle-dependent kinase Cdc28, the primary regulator of cell cycle progression. Finally, we show that CRISPRi efficiency is temperature-dependent and that different phenotypes vary in their sensitivity to knock-down. In summary, our study quantifies regulatory gene functions in different aspects of heat adaptation and advances our understanding of how eukaryotic cells counteract proteotoxic and other heat-caused damage.
Adhesion-mediated heterogeneous actin organization governs apoptotic cell extrusion
Authors: Le Anh Phuong , Rupprecht Jean-François , Mège René-Marc , Toyama Yusuke , Lim Chwee Teck , Ladoux Benoît 
Published: August 2020

ABSTRACTApoptotic extrusion is crucial in maintaining epithelial homeostasis and has implications in diseases of epithelial tissues. Current literature supports that epithelia respond to extrusion to maintain their integrity by the formation of a supracellular actomyosin ring (purse-string) in the neighbors that encompasses the dying cells. However, little is known about whether other types of actin structures could contribute to extrusion as well as how forces generated by mechanosensitive proteins in the cells are integrated. Here, we found that during extrusion, a heterogeneous actin network composed of lamellipodia protrusions and discontinuous actomyosin cables, was reorganized in the neighboring cells and was the main factor driving extrusion forwards. The early presence of basal lamellipodia protrusion participated both in basal sealing of the extrusion site and in orienting the actomyosin purse-string at the later stage of extrusion. These sequential events are essential in ensuring a successful extrusion in apicobasal direction. The co-existence of these two mechanisms is determined by the interplay between the cell-cell and cell-substrate adhesions. A theoretical model integrates the role of these cellular mechanosensitive components to explain why a dual-mode mechanism, which combined lamellipodia protrusion and purse-string contractility, leads to more efficient extrusion than a single-mode mechanism. We anticipate that our approach will be useful to provide mechanistic insight into epithelial homeostasis, morphogenetic events and tumorigenesis.
Identification and characterization of drug resistance mechanisms in cancer cells against Aurora kinase inhibitors CYC116 and ZM447439
Authors: Kollareddy Madhu , Zheleva Daniella , Džubák Petr , Srovnal Josef , Radová Lenka , Doležal Dalibor , Koudeláková Vladimíra , Brahmkshatriya Pathik Subhashchandra , Lepšík Martin , Hobza Pavel , Hajdúch Marián 
Published: August 2020

AbstractCYC116 is a selective Aurora kinase inhibitor that has been tested in a Phase I study in patients with advanced solid tumors. Although CYC116 has shown desirable preclinical efficacy, the potential for emergence of resistance has not been explored. We established several CYC116 resistant clones from isogenic HCT116 p53+/+ and HCT116 p53−/− cell line pairs. We also generated resistant clones towards ZM447439 (quinazoline derivative), a model Aurora inhibitor. The selected clones were 10-80 fold resistant to CYC116 and cross-resistant to other synthetic Aurora inhibitors including AZD1152, VX-680, and MLN8054. Resistant clones displayed multidrug resistant phenotypes, tested by using 13 major cytostatics. All clones were highly resistant to etoposide followed by other drugs. Interestingly, all CYC116 clones but not ZM447439 became polyploid. ZM447439, but not CYC116 induced three novel mutations in Aurora B. Leu152Ser significantly affected ZM447439 binding, but not CYC116. Gene expression studies revealed differential expression of more than 200 genes. Some of these genes expression profiles were also observed in CYC116 resistant primary tumors. Bcl-xL (BCL2L1) was found to be overexpressed in CYC116 clones and its knockdown resensitized the p53+/+ resistant clone to CYC116. Finally Bcl-xL overexpressing p53+/+ CYC116 clones were highly sensitive to navitoclax (ABT-263) compared to parent cells. The data shed light on the genetic basis for resistance to Aurora kinase inhibitors which could be used to predict clinical response, to select patients who might benefit from therapy and to suggest suitable drug combinations for a particular patient population.
Cryo-electron microscopy structures of pyrene-labeled ADP-Pi- and ADP-actin filaments
Authors: Chou Steven Z. , Pollard Thomas D. 
Published: August 2020

AbstractWe report high resolution cryo-electron microscopy structures of actin filaments with N-1-pyrene conjugated to cysteine 374 and either ADP (3.2 Å) or ADP-phosphate (3.0 Å) in the active site. Polymerization buries pyrene in a hydrophobic cavity between subunits along the long-pitch helix with only minor differences in conformation compared with native actin filaments. These structures explain how polymerization increases the fluorescence 20-fold, how myosin and cofilin binding to filaments reduces the fluorescence and how profilin binding to actin monomers increases the fluorescence.
University students' preference regarding social media content, internet usage and online friendship patterns
Authors: Butt Asma 
Published: August 2020

PurposeThis study aimed to find out the web content accessed by university students and to compare the level of interaction with real-life friends and online friends.Design/methodology/approachIn this study, the quantitative research design used, and the researcher collected data through the survey method. The population comprises all undergraduate students at the University of the Punjab, Lahore. The sample of 320 students, age ranges from 18 to 22 years from eight selected departments, collected through a simple random sampling technique and after extraction 284 questionnaires evaluated by using Statistical Package for Social Sciences (SPSS).FindingsThe findings of the study showed that students preferred activities on the Internet is to access social networking sites. Additionally, the mobile phone is the most commonly used device among university students to access the Internet. Furthermore, students mostly used Facebook to keep in touch with their old friends and talk on different topics more easily with their online friends as compared to real-life friends. The study also shows that the results of both the hypothesis are significant; therefore, no difference exists regarding time spent on the Internet in real-life friendship patterns and online friendship patterns.Originality/valueThe research was used to find out the difference between the online friendship and real-life friendship patterns of the two groups who use the Internet for less time and who spend more time on the Internet among the university students.
What determines forward-looking information disclosure in Bangladesh?
Authors: Dey Pappu Kumar , Roy Manas , Akter Mohsina 
Published: August 2020

PurposeThe study aims to examine the level and extent of forward-looking information (FLI) disclosure and identify the determinants driving the FLI disclosure (FLID) in the context of an emerging and developing economy.Design/methodology/approachThe sample includes annual reports of the top 30 listed companies in Bangladesh for the years 2013–2017. The content analysis approach is used to examine the practice of FLID and to determine the extent of FLID based on the index. Multiple linear regression analysis is performed to identify the determinants of FLID.FindingsThis research finds that board size, auditor's global affiliation, leverage and profitability have a substantial positive impact on FLID. By contrast, firm size and listing age have a significant negative association with FLID. Moreover, contrary to our expectation, female representation in the boardroom has an inverse effect on FLID. This study, however, does not suggest any significant impact of board independence.Research limitations/implicationsSmall sample size may limit the generalizability of the findings. Besides, the FLID index score may be affected by the subjective judgment while analyzing the content of the annual report.Practical implicationsThe findings of this paper may assist the regulators and policymakers in incorporating this new reporting paradigm in regulations. Alternatively, the current research can serve as a basis to further understand the importance of FLID for the stakeholders.Originality/valueThis empirical study contributes to the current FLI literature in Bangladesh. A handful of studies have been done to examine the nature and level of FLID and find out the determinants of FLID in the developing countries. To the best of the authors' knowledge, no study yet has been explored on FLID and its determinants by classifying them as qualitative and quantitative in Bangladesh.
The nature and extent of foresight-infused strategy: a case study highlighting the liberal arts academy’s future move from traditional education
Authors: Klakurka Jan , Irwin Bill 
Published: August 2020

Purpose
Over the past seven years within a small, liberal arts (LA) Canadian university, significant paradigm shifts in students’ programmatic choices have occurred reflecting student preference for business-related programs versus traditional LA offerings. Grounded in strategic foresight (SF) practices, this paper aims to investigate drivers of declining traditional LA enrolment that are currently a boon for management studies, positing implications for long-term futures of the LA Academy.

Design/methodology/approach
This paper lays out foundational research exploring phenomena in the academy, including disruptive forces, and explores how SF can clarify and shape long-term choices. Seeking to answer what paradigm-shifting forces really mean for the future of the academy, a case study approach is used to interpret disruptions to a Canadian institution facing present challenges and an uncertain future. Scenarios are developed for the broader academy using an environmental understanding to better inform predictive actions envisioned in academic institutional future planning.

Findings
The outcome of this research, including four scenarios, will be used to better understand student and stakeholder motivations informing future academic planning. As institutional paradigms appear resistant to change, these foresight-inspired findings are valuable considerations for institutional administrators, particularly those at stressed organizations facing unsettling realities.

Originality/value
The case study identifies that for the LA Academy, myriad future unknowns exist, including its continued existence in today’s form. Institutions are generally unresponsive to the precursors of future change and are not systematically exploring future options.
Return to work after maternity leave: the role of support policies on work attitudes of women in management positions
Authors: Costantini Arianna , Dickert Stephan , Sartori Riccardo , Ceschi Andrea 
Published: August 2020

PurposeThis study aims to expand our knowledge on the processes through which work–family policies relate to work–family conflict as well as work–related attitudes among women in management positions returning to work after maternity leave.Design/methodology/approachA total of 238 women in management positions who recently have returned to work after maternity leave completed a self-reported questionnaire.FindingsResults show that the availability of policies was either directly or indirectly positively related to work attitudes among female managers. Also, findings show that work–family conflict partially mediates the relationship between the availability of communication and psychological support and flexible time management policies with work engagement, and policy availability moderates the relationship between work–family conflict and work engagement.Originality/valueManagers have a crucial role in conveying the value of work–family policies and in creating a culture supporting the management of work and family. By investigating the processes underlying the role of work–family policies in influencing work attitudes of women in managerial positions, this study sheds light on how the awareness of the available policies might be an important determinant of work-related well-being and organizational commitment.
Foreign trade, financial development, agriculture, energy consumption and CO2 emission: testing EKC among emerging economies
Authors: Koshta Nitin , Bashir Hajam Abid , Samad Taab Ahmad 
Published: August 2020

Purpose
The main purpose of this study is to explore the presence of the EKC hypothesis in emerging economies. Additionally, the present study also explores the existence of the “resource curse hypothesis” (RCH), and the causal relationship among the variables that are considered for testing the presence of EKC and RCH hypothesis for a panel of selected emerging economies for the time period between 1990 and 2014.

Design/methodology/approach
The authors performed unit root test followed by cointegration test to test the existence of cointegrating relationship among the variables. Dynamic ordinary least square (DOLS) and fully modified ordinary least square (FMOLS) methods are used to obtain long-run estimates of considered variables, and the Granger causality test is performed to test the directional causality.

Findings
The long-run estimates obtained from DOLS and FMOLS techniques support the presence of the EKC (inverted U-shape) and the RCH.

Originality/value
To the best of the authors’ knowledge, the present work is the pioneer study for EKC and RCH investigation in the context of emerging economies. The policy implication is that these economies should look forward to drafting new policies to reduce environmental degradation and promote sustainable development.
Tribological behavior of oils additised with a phosphonium-derived ionic liquid compared to a commercial oil
Authors: Bartolomé Sáez Marlene , Hernández Battez Antolin E. , Espina Casado Jorge , Viesca Rodríguez José L. , Fernández-González Alfonso , González Rodriguez Rubén 
Published: August 2020

Purpose
The purpose of this paper is to study the antifriction, antiwear and tribolayer formation properties of the trihexyltetradecylphosphonium bis(2,4,4-trimethylpentyl) phosphinate ionic liquid (IL) as additive at 1 wt.% in two base oils and their mixtures, comparing the results with those of a commercial oil.

Design/methodology/approach
The mixture of the base oils used in the formulation of the commercial oil SAE 0W20 plus the IL was tested under rolling/sliding and reciprocating conditions to determine the so-called Stribeck curve, the tribolayer formation and the antifriction and antiwear behaviors.

Findings
The use of this IL as additive in these oils does not change their viscosity; improves the antifriction and antiwear properties of the base oils, making equal or outperforming these properties of the SAE 0W20; and the thickness and formation rate of the tribolayer resulting from the IL-surface interaction is highly dependent on the type of base oil and influence on the friction and wear results.

Originality/value
The use of this IL allows to replace partial or totally commercial antifriction and antiwear additives.

Peer review
The peer review history for this article is available at: https://publons.com/publon/10.1108/ILT-05-2020-0179/