bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparison of Unipedicular and Bipedicular Percutaneous Kyphoplasty for Kummell's Disease
Authors: Pan Dan , Ouyang Xiaojie , Huang Qinghua , Chen Dayong 
Published: December 2020

Abstract
Background: Percutaneous kyphoplasty (PKP) is effective for the treatment of Kummell's disease. However, controversy remains regarding whether a unipedicular or bipedicular PKP is superior.Methods: A retrospective study was performed to review 40 patients with stage I and II Kummell's disease who underwent PKP in our hospital from January 2015 to June 2018. Based on the transpedicular approach of PKP, those patients were randomly divided into unipedicular group (n = 19) and bipedicular group (n = 21) . Operative time, bone cement injection volume and cement leakage rate were compared in the two groups. Pre- and post-operative visual analogue score (VAS), local kyphotic angle and average vertebral height were also evaluated.Results: All patients underwent surgery successfully. Compared with preoperative condition, VAS was significantly decreased at 1 day after operation and the last follow-up in both groups (P < 0.05) , and local kyphotic angle and average vertebral height were restored markedly (P < 0.05). Operative time of both groups had no significant difference (P > 0.05). Bone cement injection volume was larger in bipedicular group (P < 0.05). At 1 day after operation and the last follow-up , the local kyphotic angle and average vertebral height in bipedicular group were restored better than those in unipedicular group (P < 0.05). There were 4 cases of cement leakage in both groups, with leakage rates of 21.1% and 19.0%, respectively, and the difference was not significant (P > 0.05).Conclusion: Both unipedicular and bipedicular PKP are effective for treating patients with stage I and II Kummell's disease, while postoperative pain relief and imaging results in bipedicular group were better than those in unipedicular group.
A High Throughput Method to Analyze the Interaction Proteins With p22 Protein of African Swine Fever Virus in Vitro
Authors: Zhu Xuejiao , Fan Baochao , Zhou Junming , Wang Dandan , Fan Huiying , Li Bin 
Published: December 2020

Abstract
Background: African Swine Fever Virus (ASFV) has been identified as the agent of ASF, which has resulting in a mortality rate of nearly 100% in domestic pigs worldwide. Protein p22 encoded by pKP177L was reported to be localized at the inner envelope of the virus, while the function of p22 remains unclear.Methods: Protein p22 interacted proteins of the host were immune-precipitated and identified by Liquid Chromatography Mass Spectrometry, analyzed by Go terms and KEGG pathways. Results: Numerous cellular proteins in 293-T that interacted with p22 protein were identified. These interacted proteins were related to the biological processes of binding, cell structure, signal transduction, cell adhesion, etc., and their interactions. At the same time, the interacted proteins participated in several KEGG pathways like ribosome, splicesome, etc. The key proteins in PPI network were closely related to actin filament organization and movement, resulting in affecting the process of phagocytosis and endocytosis. Conclusions: The identified high number of proteins interacted with p22 and as a large database should be very useful to elucidate the function of p22 in the near future, and lay the foundation for elucidating the mechanism of ASFV.
CPT1B, a Metabolic Molecule, Is Also an Independent Risk Factor in CN-AML
Authors: Ling Qing , Mao Shihui , Pan Jiajia , Wei Wenwen , Qian Yu , Li Fenglin , Huang Shujuan , Ye Wenle , Lin Xiangjie , Huang Jiansong , Wang Jinghan , Jin Jie 
Published: December 2020

Abstract
Background: In recent years, fatty acid oxidation has been considered as an important energy source for tumorigenesis and development. There are extensive studies on CPT1A, a kind of fatty acid oxidation rate-limiting enzyme. However, prognostic value and regulatory network of another subtype CPT1B in AML remains elusive. This submission aims to clarify the independent prognostic role of the metabolic molecule CPT1B in CN-AML from cilinical data and molecule levels including mRNA, miRNA and lncRNA. Method: First, we analysed the CPT1B expression in AML conhort via online database “GEPIA”. Following the assessments on differential mRNA, miRNA, lncRNA expression analysis on the paired CPT1B high and low expression groups, there were abnormal changes at different molecular levels. Moreover, we determine the preliminary predictions on the regulatory network of CPT1B in AML by constructing miRNA-mRNA and ceRNA networks. The study had yielded a series of molecules that potentially proved the prognostic prediction and metabolic function of CPT1B from the side. Finally, we analysed the CPT1B expression in our own conhort to make a verification. Result: Notably, there was a significantly high expression of CPT1B in AML patients than normal people and was associated with the poor outcome. Molecules from different levels verified our finding and revealed the possible regulatory mechanism of CPT1B in AML. Conclusion: CPT1B is a potential prognostic factor as well as a therapeutic target of AML.
Mediation Analysis of the Role of Air Pollution on the Relationship between Road Network Density and Cognitive Function: Results from a Cross-Sectional Study among Adults Age 60 and Older in Liaoning, China
Authors: Bai Xue , Wei Jiaxin , Luan Dechun , Liu Mengting , Gong Yonghui , Gao Qian 
Published: December 2020

Abstract
Background: Research on the relationship between road network density and cognitive function is lacking, and air pollution is related to both issues.Objective: We examined the mediating role of air pollution in relationship to road network density and cognitive function of older adults in Liaoning, China.Methods: This analysis included 745 adults age 60 and older. We obtained road network density for total roads, main roads, secondary roads, and other roads by OpenStreetMap and QGIS. We used air quality index (AQI) for the whole year, heating period, and nonheating period to assess individual air pollution exposure levels in different periods. We measured cognitive function using the Mini-Mental State Examination (MMSE). We used mediation analysis to evaluate the role of air pollution in the relationship between road network density and cognitive function.Results: The relationship between total road network density and global cognitive function was positive (β>0, p<0.01), and air pollution had a suppressing effect. The main road network density and cognitive function were negatively correlated, and the mediating effect of air pollution for all three periods accounted for 81.69%–90.09% of the total effects. The other road network density and cognitive function were positively correlated, and the suppressing effect of air pollution for all three periods accounted for 10.06%–18.50% of the total effects. The secondary road network density and cognitive function were positively correlated, and the suppressing effect of air pollution in the whole year and heating period accounted for 21.06% and 26.10% of the total effects, respectively. During nonheating period, the suppressing effect of air pollution was not statistically significant [β = −0.018; standard error (SE) = 0.014; 95% confidence interval (CI): −0.046, 0.007].Conclusion: Because the different categories of road network density have different relationships with cognitive function, air pollution had different effects on these relationships.
Identifying Genetic Variants Associated With Noise-induced Hearing Loss Based on a Novel Strategy for Evaluating Individual Susceptibility
Authors: Jiang Zhuang , Fa Botao , Zhang Xunmiao , Feng Yanmei , Wang Jiping , Shi Haibo , Sun Daoyuan , Wang Hui , Yin Shankai 
Published: December 2020

Abstract
Background: The overall genetic profile for noise-induced hearing loss (NIHL) remains to be explored. Here we used a novel machine learning (ML) strategy to evaluate individual susceptibility to NIHL and identify the underlying genetic variants based on a subsample of participants with extreme phenotype. Methods: Demographic and audiometric data of 5,539 shipbuilding workers from large cross-sectional surveys were included in four ML algorithms to predict the hearing level. The area under the curve (AUC) and prediction accuracy were used to assess the performance of the classification models. We screened 300 participants that were misclassified by all of the four ML models, with extreme phenotypes implying they were either highly susceptible or resistant to NIHL and used whole-exome sequencing (WES) to identify the underlying variants associated with NIHL risk among the NIHL-susceptible and NIHL-resistant individuals. Subsequently, candidate risk loci were validated in a large independent noise-exposed cohort, followed by a meta-analysis.Results: With 10-fold cross-validation, the performances of the four ML models were robust and similar, with average AUC and accuracy ranging from 0.783 to 0.798 and 73.7% to 73.8%, respectively. The phenotypes of the NIHL-susceptible group and NIHL-resistant group were significantly different (all p<0.001). After WES analysis and filtering, 12 novel variants contributing to NIHL susceptibility were identified and replicated. The meta-analyses shown that the rs41281334 A allele of CDH23 (OR=1.506, 95% CI=1.106-2.051) and the rs12339210 C allele of WHRN (OR=3.06, 95% CI=1.398-6.700) were significantly associated with increased risk of NIHL after adjustment for conventional risk factors.Conclusions: This study determined two novel genetic variants in CDH23 rs41281334 and WHRN rs12339210 associated with NIHL risk, based on a potential approach for evaluating individual susceptibility using ML models. Trial registration: Chinese Clinical Trial Registry (registration number: ChiCTR-RPC-17012580)
Changing Trends in Lipid Profile and Biomarkers of Renal Function and Bone Metabolism Before and After Switching From Tenofovir Disoproxil Fumarate to Tenofovir Alafenamide: a Prospective Observational Study
Authors: Ikeda Mahoko , Wakabayashi Yoshitaka , Okamoto Koh , Yanagimoto Shintaro , Okugawa Shu , Moriya Kyoji 
Published: December 2020

Abstract
BackgroundAntiretrovirals, including tenofovir, can suppress human immunodeficiency virus (HIV) infection but cannot completely eradicate it. Patients with HIV infection are administered antiretroviral drugs over a long term; thus, managing consequent adverse drug reactions, such as renal dysfunction and bone mineral loss, is important. Currently, highly sensitive biomarkers that can detect adverse drug reactions early have not been well studied. MethodsThis single-center, prospective, observational study explored changes in the biomarkers of renal function, bone metabolism, and lipid profile before and after switching from tenofovir disoproxil fumarate (TDF) to tenofovir alafenamide (TAF) in patients with HIV infection. ResultsAll 31 enrolled patients had been treated with antiretrovirals for more than 5 years. The urinary liver-type fatty acid binding protein (L-FABP)/creatinine ratio was significantly decreased at 3 and 6 months after switching to TAF compared with that before switching to TAF (-0.5μg/g･Cr at 3 months, and -0.8μg/g･Cr at 6 months; p <005 for both at 3 and 6 months) . The urinary N-terminal telopeptide (NTx)/creatinine ratio decreased over the study period, and the ratios were significantly different between 3 months and 6 months (-11 nmol/mmol Cr at 3 months, -15.2 nmol/mmol Cr at 6 months; p =0.0069 at 3 months, p<.0001 at 6 months) . Low density lipoprotein-cholesterol level significantly increased at 3 (+26 mg/dL) and 6 months (+13 mg/dL) compared with that at the baseline (p < 0.0001).ConclusionsSwitching from TDF to TAF decreased the levels of renal and bone biomarkers, such as urinary L-FABP and NTx, but increased low density lipoprotein-cholesterol levels. Highly sensitive markers, such as urinary L-FABP and NTx, might be useful tools to predict adverse drug reaction early.
Modest Evolutionary Changes of the SARS-CoV-2 Genome in Bangladesh
Authors: Rahman Sezanur , Hasan Mehedi , Hossain Mohammad Enayet , Rahman Mohammed Ziaur , Rahman Mustafizur 
Published: December 2020

Abstract
Background: The human-to-human transmissive nature of SARS-CoV-2 makes Bangladesh, as well as the other South Asian regions, vulnerable to the ongoing pandemic of COVID-19 due to their high population densities. The present study was designed based on the genome wide analysis of Bangladeshi and other South Asian isolates. Complete sequences of SARS-CoV-2 were retrieved from the EpiCoV database in order to identify molecular features demonstrating the evolutionary trail and mutation rate.Result: The complete genome mutation rate of the Bangladeshi isolates was estimated to be 0.49E-3 nucleotide substitutions/site/year. A higher mutation rate was found in the non-structural protein-coding genes at: ORF6 (10.29E-3), ORF7a (31.81E-3), and ORF8 (18.35E-3). In contrast, the mutation rates of the structural protein-coding genes were relatively low at: M (1.14E-3), S (1.47E-3), E (3.35E-3), and N (4.59E-3).Conclusions: A comparison of Bangladeshi and other South Asian isolates demonstrated that there were limited mutational changes in the SARS CoV-2 genome. Knowledge of the Southeast Asian SARS CoV-2 evolutionary genome will help in selecting future vaccine candidates and designing therapeutic drug targets.
MiR-191-5p Is Upregulated in Culture Media of Implanted Human Embryo on Day Three of Development
Authors: Acuña-González Ricardo Josue , Morales-Hernández Fela Vanesa , López-Canales Jorge Skiold , Lozano-Cuenca Jair , Osorio-Caballero Mauricio , Flores-Herrera Héctor 
Published: December 2020

Abstract
Background: Morphologic features are the most common criteria for selecting human embryo to be transferred to the receptive uterine cavity. However, such characteristics are not valid for embryos in cellular arrest. The aim of this study was to quantify the expression profile of hsa-miR-21-3p, -24-1-5p, -191-5p, and -372-5p on day 3 of culture media from in vitro fertilization (IVF) embryo that were implanted or failed to be implanted in patients (n=25 pregnant and 25, non-pregnant patients). Methods: Fifty patients were accepted in the Department of Reproductive Biology of a Hospital in México City, based on the Institutional inclusion criteria for in vitro fertilization. On day 3 of development, embryos were transferred to women, and the culture medium was collected from implanted embryos (n=25, pregnant patients) and non-implanted embryos (n=25, non-pregnant). In the culture medium, RNA was isolated using TRIzol reagent. MiRNA expression was detected through RT-PCR with specific primers. Expression bands were quantified using an optic density.Results: The expression profiles were compared between pregnant and non-pregnant patients revealing a significant 5.2-fold greater expression of hsa-miR-191-5p in the former group (p ≤0.001) and a significantly higher expression of hsa-miR-24-1-5p (p =0.043) in the latter. No significant difference was found between the two groups in regard hsa-miR-21-3p or hsa-miR-372-5p (p =0.41). Conclusions: According to the results, has-miR-191-5p could possibly be a possible biomarker of adequate human embryo development. This miRNA modulated IGF2BP-1 and IGF2R, which are associated with the implantation window. On the other hand, hsa-miR-24-1-5p may be related to a poor prognosis of human embryo development.
Factors Associated with Alcohol Consumption Among Youth Men, Ethiopian 2016 Demographic Health Survey.
Authors: Tegegne Kindie Mekuria , Abate Biruk Beletew , Kassaw Mesfine Wudu , G/mariam Etsay Hailu , Yimam Mohammed Ahmed 
Published: December 2020

Abstract
Background: Alcohol consumption is a global issue, both developed and underdeveloped countries' health trouble. Specifically in Ethiopia, youth men 15-24 years of age are engaged in psychoactive substances mainly alcohols. Alcoholic person has other concomitant mental illness the discord of school, unemployment and being low socio-economic status.Methods: A cross-sectional study using the 2016 Ethiopia Demographic and Health Survey (EDHS) data set was applied. The samples were selected using a two-stage stratified cluster sampling technique. The data were analyzed with SPSS version 20. Multivariate logistic regression analysis was performed to identify factors associated with alcohol consumption by controlling confounders. An adjusted odds ratio with a 95 % confidence interval was considered to declare a statistically significant association.Results: The present study comprised of 4486 weight samples of youth men. The prevalence of alcohol consumption among youth men in this study was 39.3 %. Men aged 15-19 (AOR=1.27 (95% CI =1.01-1.60), being unemployed (AOR=1.62 (95% CI=1.27-2.07), don’t have awareness about HIV/AIDS (AOR=4.70 (95%=2.44-9.01),being married (AOD 0.58(0.39-0.85) and poorer youth men (AOD =1.75(95%CI=1.21-2.54) ,being orthodox (AOR=5.12(95%CI =2.47-10.64), protestant (AOR=0.42(95% CI =0.20- 0.87)Muslim (AOR=0.07(95% CI=0.03-0.16) religious followers were significantly associated with alcohol consumption.Conclusion: Alcohol consumption among youth men was relatively high when we compared with other countries study. Strengthening the awareness of HIV/AIDS, change alcohol drinking experience on religious ceremonies especially in orthodox, organizes the youth and job creation is suggested to avert alcohol consumption trajectory.
Gender Differences in Mental Health Outcomes Amid the COVID-19 Pandemic and a Collapsing Economy: A Cross-Sectional Study
Authors: Hajj Aline , Badro Danielle A , Selwan Carla Abou , Sacre Hala , Aoun Randa , Salameh Pascale 
Published: December 2020

Abstract

Background: Emergency plans to contain the COVID-19 failed to take into consideration the repercussions of the combined economic and sanitary crisis among the most vulnerable populations who might be disproportionately affected, including women and abused people. This study primarily aimed to assess the effects of the COVID-19 and economy-related variables on Lebanese adults’ mental health, focusing on gender differences.Methods: An online-based cross-sectional study was conducted between May 10-20, 2020, using validated scales. All adults living in Lebanon, with access to the internet, were eligible, regardless of their age, socioeconomic status, race, ethnicity, nationality, sex, gender identity, religion, geography, ability, and culture (n=502).Results: The multivariate analysis, adjusted on sociodemographic characteristics, economic, and coronavirus-related variables, showed that women exhibit higher levels of stress, anxiety, and post-traumatic stress symptoms (PTSS) than men. Moreover, violence at home was associated with more stress and insomnia, with borderline results for anxiety and well-being. No significant difference was found for PTSS. Conclusion: In the context of combined health and economic crises, mental health outcomes are not equally felt by women. Additional studies in a few months would help better understand the long-term impact, especially on PTSS and QOL, which might not be perceptible during the crisis. Focused public health strategies are warranted to support and protect the most vulnerable populations.
Cross-Regional Research in Demographic Impact on Safety Consciousness and Safety Citizenship Behavior of Construction Workers: A Comparative Study between Mainland China and Hong Kong
Authors: MENG Xiangcheng , CHAN Alan H.S. 
Published: December 2020

Abstract
Background: The construction industry has rapidly developed with the continuous prosperity in Hong Kong and Mainland China, though accidents still occur with unacceptable frequency and severity. For promoting the safety issue of workers in construction industry, safety citizenship behavior (SCB) and safety consciousness (SC) were considered two influential constructs and further studied with integration of sociodemographic theories by scholars. However, no study has compared the SC and SCB of construction workers in terms of the demographic influence between Mainland China and Hong Kong. Methods: To fill this research gap, this study investigated the territorial difference between these two regions by conducting a cross-sectional questionnaire survey with recruitment of 253 Mainland construction workers and 256 Hong Kong construction workers. Results: Significant similarities and differences of SC and SCB performance were revealed in terms of the workers with different genders, education levels, weekly working hours, and ages. Conclusions: This study provides insights into the comparison of demographic influence on SC and SCB of construction workers between Hong Kong and Mainland China, which is unique as it can yield useful managerial knowledge relevant to the personal safety of targeted groups of construction workers with particular demographic characteristic in both regions and contribute the implementation of safety interventions in line with the specific distinction in territorial aspect.
Predictors for Uptake of Tetanus Toxoid Vaccination During Pregnancy Among Women of Reproductive Age in Tanzania; An Analysis of Data from the 2015-16 Tanzania Demographic and Health Survey and Malaria Indicators Survey
Authors: Tungaraza Maximillian Biyemo , Moshi Fabiola Vicent 
Published: December 2020

Abstract
Background: Tetanus is a vaccine preventable disease. Tanzania through its Ministry of Health, Community Development, Gender, Elderly and Children (MoHCDGEC) recommend all women should receive at least five TT doses in their reproductive life. Little is known on predictors of TT uptake during pregnancy among women of reproductive age in TanzaniaMethod: The study used data from the 2015-16 Tanzania Demographic and Health Survey and Malaria Indicators Survey (2015-16 TDHS-MIS). A total of 6924women of active reproductive age from 15 to 49 were included in the analysis. Both univariate and multiple regression analyses were used to determine the predictors of TT uptake during pregnancy among women of reproductive age in Tanzania.Results: More than half, 3480 (50.3%) of the study population had either no or one tetanus injection. A total of 3444 (49.7%) had two or more tetanus injection during pregnancy. After adjusted for confounders, predictors of uptake of tetanus vaccination were early antenatal booking (AOR=1.174 at 95% CI=1.033-1.335, p=0.014), age group of women [ 20 to 34 years (AOR=1.433 at 95% CI= 1.155-1.778, p=0.001), more than 34 years (AOR=1.379 at 95% CI=1.065-1.786, p=0.015)], wealth index [ rich (AOR=1.261 at 95% CI= 1.083-1.468, p = 0.003)], parity [para 2-4 (AOR=0.401 at 95% CI=0.343-0.468, p<0.001), para 5 and above (AOR=0.217 at 95% CI=0.178-0.265, p<0.001)], level of education [ primary level, (AOR=0.864 at 95% CI=0.754-0.99, p=0.035)] zones [Unguja Zanzibar Island (AOR=0.434 at 95% CI=0.309-0.609, p<0.001), Pemba (Pemba Island) (AOR=0.34 at 95% CI=0.226-0.512, p<0.001)] and adequate ANC visits (AOR=0.649 at 95% CI=0.582-0.723, p<0.001)Conclusion: Antenatal care service utilization, including both timeliness and the number of ANC visits attended were found important predictors for TT vaccine uptake. Therefore, the responsible ministry of health in the country should strengthen the strategies in place to increase maternal awareness on importance of ANC service utilization.