bluuebunny/crossref_metadata_2025_split huggingface dataset:
Insights into Functional Connectivity in Mammalian Signal Transduction Pathways by Pairwise Comparison of Protein Interaction Partners of Critical Signaling Hubs
Authors: Ramana Chilakamarti V. 
Published: December 2019

AbstractGrowth factors and cytokines activate signal transduction pathways and regulate gene expression in eukaryotes. Intracellular domains of activated receptors recruit several protein kinases as well as transcription factors that serve as platforms or hubs for the assembly of multi-protein complexes. The signaling hubs involved in a related biologic function often share common interaction proteins and target genes. This functional connectivity suggests that a pairwise comparison of protein interaction partners of signaling hubs and network analysis of common partners and their expression analysis might lead to the identification of critical nodes in cellular signaling. A pairwise comparison of signaling hubs across several related pathways might also reveal novel signaling modules. Analysis ofProteinInteractionConnectome byVenn (PIC-VENN) of transcription factors STAT1, STAT3, NFKB1, RELA, FOS and JUN, and their common interaction network suggested that BRCA1 and TSC22D3 function as critical nodes in immune responses by connecting the signaling nodes into signaling modules. Mutations or differential expression levels of these critical nodes in pathological conditions might deregulate signaling pathways and their target genes involved in inflammation. Biological connectivity emerges from the structural connectivity of interaction networks across several signaling hubs in related pathways. Application of PIC-VENN to several signaling hubs might reveal novel nodes and modules that can be targeted to simultaneously activate or inhibit cell signaling in health and disease.
Temperature sensitive Mutant Proteome Profiling: a novel tool for the characterization of the global impact of missense mutations on the proteome
Authors: Peck Justice Sarah A. , Qi Guihong , Wijeratne H. R. Sagara , Victorino José F. , Simpson Ed R. , Wijeratne Aruna B. , Mosley Amber L. 
Published: December 2019

ABSTRACTTemperature sensitive (TS) mutants have been foundational in the characterization of essential genes. However, a high-throughput workflow for characterization of biophysical changes in TS mutants is lacking.Temperature sensitiveMutantProteomeProfiling (TeMPP) is a novel application of mass spectrometry (MS) based thermal proteome profiling (TPP) to characterize effects of missense mutations on protein stability and PPIs. This study characterizes missense mutations in two different subunits of the 26S proteasome on the thermal stability of the proteome at large, revealing distinct mechanistic details that were not obtained using only steady-state transcriptome and proteome analyses. TeMPP is a precise approach to measure changes in missense mutant containing proteomes without the requirement for large amounts of starting material, specific antibodies against proteins of interest, and/or genetic manipulation of the biological system. Overall, TeMPP provides unique mechanistic insights into missense mutation dysfunction and connection of genotype to phenotype in a rapid, non-biased fashion.
Critiquing the “National Standards for School-based Initial Teacher Training Mentors” in England
Authors: Jerome Lee , Brook Victoria 
Published: December 2019

Purpose
In 2016, the National Standards for School-based Initial Teacher Training (ITT) Mentors were published in England. The purpose of this paper is to critique these standards through a comparison of how others have framed and defined the role of the mentor, drawing on equivalent standards already published in nursing (2008) and social work (2012).

Design/methodology/approach
An analysis of three sets of professional standards was conducted by adapting the “constant comparison” approach in which the researchers sought to combine a form of inductive coding with comparison across the texts. This enabled the identification of a number of common themes and omissions across the three sets of standards.

Findings
The analysis revealed the ITT mentor standards provide a comparatively limited account of the role of the mentor, particularly in relation to the process of assessment, the power dynamics between mentors and student teachers, and the school as an institutional site for professional learning.

Originality/value
The study’s originality lies in the inter-professional comparative analysis, which revealed a number of potentially contentious issues not immediately apparent from a close textual analysis of the ITT mentor standards.
Gratitude and leadership in higher education institutions
Authors: Garg Naval , Gera Shilpika 
Published: December 2019

Purpose
The purpose of this paper is to examine the relationship between gratitude and transformational leadership among university teachers. The study also intended to investigate the mediating effect of social intelligence (SI) on this relationship between gratitude and leadership.

Design/methodology/approach
Correlation and regression are used to analyze the relationship between gratitude, SI and leadership. Mediating effect of SI is accessed using Baron and Kenny’s (1986) method and bootstrapping technique.

Findings
Findings reveal positive relationship between gratitude and teacher leadership. SI is also found to mediate partially. The paper also discusses practical implications, limitations and scope for future researchers.

Originality/value
This is one of the pioneer studies that explore the role of gratitude in leadership development. Findings of the study will guide educational administrators to become more effective and efficient leaders.
Protecting intellectual property from insider threats
Authors: Kim Hyungjin Lukas , Hovav Anat , Han Jinyoung 
Published: December 2019

PurposeThe purpose of this paper is to propose a theory of information security intelligence and examine the effects of managers’ information security intelligence (MISI) on employees’ procedural countermeasure awareness and information security policy (ISP) compliance intention.Design/methodology/approachA survey approach and structural equation modeling is utilized. Partial least squares (WarpPLS 6.0) and nonlinear algorithm are employed to analyze and examine the hypotheses. In total, 324 employees from companies in South Korea participated in the survey, which was conducted by a professional survey service company.FindingsMISI positively affects employees’ awareness of information security procedural countermeasures; information security knowledge and problem-solving skills have positive effects on procedural countermeasures awareness; MISI increases employees’ compliance intention through procedural countermeasure awareness; and information security procedural countermeasures positively affect employees’ ISP compliance intention.Research limitations/implicationsThis study proposes a theory of information security intelligence and examines its impacts on employees’ compliance intentions. The study highlights the mediating role of information security procedural countermeasures between information security intelligence and employees’ compliance intentions.Practical implicationsManagers should improve and explicitly demonstrate information security knowledge and problem-solving skills to increase employees’ ISP compliance intention. To protect the organization’s intellectual capital, managers should champion the development and promotion of PCM, rather than leave these functions to the information security group.Originality/valueThis is the first empirical study to propose and validate MISI.
A theory of (research) practice makes sense in sensemaking
Authors: van Hilten Adriana 
Published: December 2019

PurposeThe purpose of this paper is to introduce Bourdieu’s social theory, and its “thinking tools” of habitus, doxa, field and capital, as a sensemaking theory.Design/methodology/approachThe emic research studied, for a particular group, the firm-wide implementation of a new system. The study used data occurring naturally in the organization (executive newsletters), and externally (third-party surveys), as well as 23 participant interviews to structure the social space (field) and determine what is of interest (identity). Interviews were coded for habitus, doxa, field, capital, symbolic violence and strategies to re-assert interviewees’ own doxa versus logic imposed by the powerful.FindingsA unique, esteemed identity was being erased through executive attempts to introduce a new culture at the firm, and the new systems represented a challenge to this valued identity. Participants used strategies to re-assert their identity through not participating in the logic of the new tool: discussing misuse, lack of use, relative unimportance and low priority of the new tool.Practical implicationsChange that threatens an esteemed, valued identity is more likely to be resisted. The logic of an established practice or system (beyond merely gathering user requirements) is beneficial in understanding potential reactions to a new system. Change in systems that occur simultaneously with the imposition of a new culture, particularly where the system is seen as being a representation of that imposed culture, may be resisted through non-practice (misuse or lack of use) of the new system.Originality/valueThe paper demonstrates the applicability of Bourdieu’s social theory to organizational studies, providing a sensemaking of change and acts of resistance.
Eight‐year post‐trial follow‐up of health care and long‐term care costs of tele‐based health coaching
Authors: Mustonen Erja , Hörhammer Iiris , Absetz Pilvikki , Patja Kristiina , Lammintakanen Johanna , Talja Martti , Kuronen Risto , Linna Miika 
Published: December 2019

AbstractObjectiveTo evaluate the long‐term effect of telephone health coaching on health care and long‐term care (LTC) costs in type 2 diabetes (T2D) and coronary artery disease (CAD) patients.Data Sources/Study SettingRandomized controlled trial (RCT) data were linked to Finnish national health and social care registries and electronic health records (EHR). Post‐trial eight‐year economic evaluation was conducted.Study DesignA total of 1,535 patients (≥45 years) were randomized to the intervention (n = 1034) and control groups (n = 501). The intervention group received monthly telephone health coaching for 12 months. Usual health care and LTC were provided for both groups.Principal FindingsIntention‐to‐treat analysis showed no significant change in total health and long‐term care costs (intervention effect €1248 [3 percent relative reduction], CI −6347 to 2217) in the intervention compared to the control group. There were also no significant changes among subgroups of patients with T2D or CAD.ConclusionsHealth coaching had a nonsignificant effect on health care and long‐term care costs in the 8‐year follow‐up among patients with T2D or CAD. More research is needed to study, which patient groups, at which state of the disease trajectory of T2D and cardiovascular disease, would best benefit from health coaching.
Dorsal striatal functional connectivity changes in Internet gaming disorder: A longitudinal magnetic resonance imaging study
Authors: Lee Deokjong , Namkoong Kee , Lee Junghan , Jung Young‐Chul 
Published: December 2019

AbstractInternet gaming disorder (IGD) is a behavioral addiction involving excessive online game use despite negative psychosocial consequences. Unrestricted online gaming may lead to changes in striatal activity and the relationship between the striatum and other cortical regions. This study investigated structural and functional abnormalities involving the striatum through longitudinal follow‐up magnetic resonance imaging (MRI) assessments. Eighteen young males with IGD (mean age: 23.8 ± 2.0 years) and 18 controls (mean age: 23.9 ± 2.7 years) were evaluated. Subjects were reassessed ≥1 year after the first visit (mean follow‐up duration: 22.8 ± 6.7 months), using voxel‐based morphometry and seed‐based resting‐state functional connectivity (FC) analyses in seed regions of the dorsal and ventral striatum. Subjects with IGD had smaller gray matter volume (GMV) in the anterior/middle cingulate cortex compared with controls during initial and follow‐up assessments. They exhibited decreased FC between the left dorsal putamen and left medial prefrontal cortex (mPFC) compared with controls. They exhibited increased FC strength between the right dorsal putamen and right middle occipital gyrus (MOG) during follow‐up. Subjects with IGD showed a significant correlation between changes in the dorsal putamen‐MOG FC and gaming time per day. Young males with IGD showed an altered FC pattern in the dorsal striatum during follow‐up. FC of the dorsal striatum in IGD increased in the mPFC and decreased in the MOG. These findings showed that IGD was accompanied by weakening of prefrontal control and strengthening of the sensorimotor network, suggesting that uncontrolled gaming may be related with functional neural changes in the dorsal striatum.
Automation, Algorithms, and Beyond: Why Work Design Matters More Than Ever in a Digital World
Authors: Parker Sharon K. , Grote Gudela 
Published: December 2019

AbstractWe propose a central role for work design in understanding the effects of digital technologies. We give examples of how new technologies can—depending on various factors—positively and negatively affect job resources (autonomy/control, skill use, job feedback, relational aspects) and job demands (e.g., performance monitoring), with consequences for employee well‐being, safety, and performance. We identify four intervention strategies. First, work design choices need to be proactively considered during technology implementation, consistent with the sociotechnical systems principle of joint optimization. Second, human‐centred design principles should be explicitly considered in the design and procurement of new technologies. Third, organizationally oriented intervention strategies need to be supported by macro‐level policies. Fourth, there is a need to go beyond a focus on upskilling employees to help them adapt to technology change, to also focus on training employees, as well as other stakeholders, in work design and related topics. Finally, we identify directions for moving the field forward, including new research questions (e.g., job autonomy in the context of machine learning; understanding designers’ work design mindsets; investigating how job crafting applies to technology); a reorientation of methods (e.g., interdisciplinary, intervention studies); and steps for achieving practical impact.
Distinctive properties of the interactions between Notch and Notch ligands
Authors: Hozumi Katsuto 
Published: December 2019

AbstractAlthough Notch signaling is known to be critical for the specification of cell fate in various developing organs, the particular roles of each Notch and Notch ligand (NotchL) have not yet been elucidated. The phenotypes found in loss‐of‐function experiments have varied, depending on the expression profiles of the receptors and ligands. However, in some cases, their significances differ from others, even with comparable levels of expression, suggesting a distinctive functional receptor‐ligand interaction during the activation process of Notch signaling. In this review, the phenotypes observed in Notch/NotchL‐deficient situations are introduced, and their distinct roles are accentuated. The distinctive features of the specific combinations of Notch/NotchL are also discussed. This review aims to highlight the unanswered questions in this field to help improve our understanding of the preferential functional interaction between Notch and NotchL.
A practical guide to DNA metabarcoding for entomological ecologists
Authors: Liu Mingxin , Clarke Laurence J. , Baker Susan C. , Jordan Gregory J. , Burridge Christopher P. 
Published: December 2019

1. DNA metabarcoding is a cost‐effective species identification approach with great potential to assist entomological ecologists. This review presents a practical guide to help entomological ecologists design their own DNA metabarcoding studies and ensure that sound ecological conclusions can be obtained.2. The review considers approaches to field sampling, laboratory work, and bioinformatic analyses, with the aim of providing the background knowledge needed to make decisions at each step of a DNA metabarcoding workflow.3. Although most conventional sampling methods can be adapted to DNA metabarcoding, this review highlights techniques that will ensure suitable DNA preservation during field sampling and laboratory storage. The review also calls for a greater understanding of the occurrence, transportation, and deposition of environmental DNA when applying DNA metabarcoding approaches for different ecosystems.4. Accurate species detection with DNA metabarcoding needs to consider biases introduced during DNA extraction and PCR amplification, cross‐contamination resulting from inappropriate amplicon library preparation, and downstream bioinformatic analyses. Quantifying species abundance with DNA metabarcoding is in its infancy, yet recent studies demonstrate promise for estimating relative species abundance from DNA sequencing reads.5. Given that bioinformatics is one of the biggest hurdles for researchers new to DNA metabarcoding, several useful graphical user interface programs are recommended for sequence data processing, and the application of emerging sequencing technologies is discussed.
Electrical stimulation of the ventral tegmental area evokes sleep‐like state transitions under urethane anaesthesia in the rat medial prefrontal cortex via dopamine D1‐like receptors
Authors: Gretenkord Sabine , Olthof Bas M. J. , Stylianou Myrto , Rees Adrian , Gartside Sarah E. , LeBeau Fiona E. N. 
Published: December 2019

AbstractThe role of dopamine in regulating sleep‐state transitions during, both natural sleep and under anaesthesia, is still unclear. Recording in vivo in the rat mPFC under urethane anaesthesia, we observed predominantly slow wave activity (SWA) of <1 Hz in the local field potential interrupted by occasional spontaneous transitions to a low‐amplitude‐fast (LAF) pattern of activity. During periods of SWA, transitions to LAF activity could be rapidly and consistently evoked by electrical stimulation of the ventral tegmental area (VTA). Spontaneous LAF activity, and that evoked by stimulation of the VTA, consisted of fast oscillations similar to those seen in the rapid eye movement (REM)‐like sleep state. Spontaneous and VTA stimulation‐evoked LAF activity occurred simultaneously along the dorsoventral extent of all mPFC subregions. Evoked LAF activity depended on VTA stimulation current and could be elicited using either regular (25–50 Hz) or burst stimulation patterns and was reproducible upon repeated stimulation. Simultaneous extracellular single‐unit recordings showed that during SWA, presumed pyramidal cells fired phasically and almost exclusively on the Up state, while during both spontaneous and VTA‐evoked LAF activity, they fired tonically. The transition to LAF activity evoked by VTA stimulation depended on dopamine D1‐like receptor activation as it was almost completely blocked by systemic administration of the D1‐like receptor antagonist SCH23390. Overall, our data demonstrate that activation of dopamine D1‐like receptors in the mPFC is important for regulating sleep‐like state transitions.
Quantification of intratumoral collagen and elastin fibers within hepatocellular carcinoma tissues finds correlations with clinico‐patho‐radiological features
Authors: Maehara Junki , Masugi Yohei , Abe Tokiya , Tsujikawa Hanako , Kurebayashi Yutaka , Ueno Akihisa , Ojima Hidenori , Okuda Shigeo , Jinzaki Masahiro , Shinoda Masahiro , Kitagawa Yuko , Oda Yoshinao , Honda Hiroshi , Sakamoto Michiie 
Published: December 2019

AimEmerging evidence suggests a promising role for tumor stromal factors in characterizing patients with various types of malignancies, including hepatocellular carcinoma (HCC). We quantified the amount of collagen and elastin fibers in HCC samples with the aim of clarifying the clinico‐patho‐radiological significance of fiber deposition in HCC.MethodsWe computed the amount of collagen and elastin fibers using digital image analysis of whole‐slide images of Elastica van Gieson‐stained tissues from 156 surgically resected HCCs. Furthermore, we assessed the correlations between the fiber content of HCC samples and clinical, pathological, and radiological features, including immunohistochemistry‐based molecular subtypes and immunosubtypes.ResultsThe intratumoral area ratio of collagen in HCC tissues (median 3.4%, range 0.1–22.2%) was more than threefold that of elastin (median 0.9%, range 0.1–9.0%); there was a strong positive correlation between the amounts of collagen and elastin. Higher levels of combined collagen and elastin were significantly associated with the confluent multinodular macroscopic tumor type, the absence of a fibrous capsule, intratumoral steatosis, scirrhous tumor stroma, dense inflammatory‐cell infiltrates, and the biliary/stem cell markers‐positive HCC subtype. The associations of higher collagen levels with radiological findings, including heterogeneous enhancement and persistent enhancement on dynamic computed tomography, were significant. In contrast, the associations of radiological findings with elastin fibers were not significant. Intratumoral fibrous stroma in HCC comprised septum‐like and perisinusoidal fibrosis; these two forms represented distinct distribution patterns of fibers and fibroblasts.ConclusionQuantitative analysis suggested that stromal fiber‐rich HCCs likely represent a distinct clinico‐patho‐radiological entity.
“We will Manage It” – Did Chancellor Merkel's Dictum Increase or Even Cause the Refugee Movement in 2015?
Authors: Pries Ludger 
Published: December 2019

AbstractIn public and scientific debates about the dynamics of the so‐called refugee crisis of 2015, there are highly controversial positions concerning the statement on August 2015 by Chancellor Merkel: “We will manage it” Did this dictum actually increase or even unleash the refugee migration towards Europe in 2015? Was Germany's governmental policy against the legal European and national frame? Based on an analysis of legal documents and public statements, the article first reconstructs the development of refugee immigration into the EU, especially to Germany, in 2015 and reviews whether the German government violated law and opened one‐sided borders. Based on survey data of 4,500 refugees in 2016, it then analyses whether the Merkel dictum significantly changed the volume and the reasons why refugees decided to flee to Germany. Finally, some conclusions are drawn.