bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dextran sulphate-induced tau assemblies cause endogenous tau aggregation and propagation in wild-type mice
Authors: Masuda-Suzukake Masami , Suzuki Genjiro , Hosokawa Masato , Nonaka Takashi , Goedert Michel , Hasegawa Masato 
Published: July 2020

AbstractAccumulation of assembled tau protein in the central nervous system is characteristic of Alzheimer’s disease and several other neurodegenerative diseases, called tauopathies. Recent studies have revealed that propagation of assembled tau is key to understanding the pathological mechanisms of these diseases. Mouse models of tau propagation are established by injecting human-derived tau seeds intracerebrally; nevertheless, these have a limitation in terms of regulation of availability. To date, no study has shown that synthetic assembled tau induce tau propagation in non-transgenic mice. Here we confirm that dextran sulphate, a sulphated glycosaminoglycan, induces the assembly of recombinant tau protein into filaments in vitro. As compared to tau filaments induced by heparin, those induced by dextran sulphate showed higher thioflavin T fluorescence and lower resistance to guanidine hydrochloride, which suggests that the two types of filaments have distinct conformational features. Unlike other synthetic filament seeds, intracerebral injection of dextran sulphate-induced assemblies of recombinant tau caused aggregation of endogenous murine tau in wild-type mice. AT8-positive tau was present at the injection site 1 month after injection, from where it spread to anatomically connected regions. Induced tau assemblies were also stained by anti-tau antibodies AT100, AT180, 12E8, PHF1, anti-pS396 and anti-pS422. They were thioflavin- and Gallyas-Braak silver-positive, indicative of amyloid. In biochemical analyses, accumulated sarkosyl-insoluble and hyperphosphorylated tau was observed in the injected mice. In conclusion, we revealed that intracerebral injection of synthetic full-length wild-type tau seeds prepared in the presence of dextran sulphate caused tau propagation in non-transgenic mice. These findings establish that propagation of tau assemblies does not require tau to be either mutant and/or overexpressed.
Neural correlates of polygenic risk score for autism spectrum disorders in general population
Authors: Khundrakpam Budhachandra , Vainik Uku , Gong Jinnan , Al-Sharif Noor , Bhutani Neha , Kiar Gregory , Zeighami Yashar , Kirschner Matthias , Luo Cheng , Dagher Alain , Evans Alan 
Published: July 2020

AbstractAutism spectrum disorder is a highly prevalent and highly heritable neurodevelopmental condition, but studies have mostly taken traditional categorical diagnosis approach (yes/no for autism spectrum disorder). In contrast, an emerging notion suggests a continuum model of autism spectrum disorder with a normal distribution of autistic tendencies in the general population, where a full diagnosis is at the severe tail of the distribution. We set out to investigate such a viewpoint by investigating the interaction of polygenic risk scores for autism spectrum disorder and Age2 on neuroimaging measures (cortical thickness and white matter connectivity) in a general population (n = 391, with age ranging from 3 to 21 years from the Pediatric Imaging, Neurocognition and Genetics study). We observed that children with higher polygenic risk for autism spectrum disorder exhibited greater cortical thickness for a large age span starting from 3 years up to ∼14 years in several cortical regions localized in bilateral precentral gyri and the left hemispheric postcentral gyrus and precuneus. In an independent case–control dataset from the Autism Brain Imaging Data Exchange (n = 560), we observed a similar pattern: children with autism spectrum disorder exhibited greater cortical thickness starting from 6 years onwards till ∼14 years in wide-spread cortical regions including (the ones identified using the general population). We also observed statistically significant regional overlap between the two maps, suggesting that some of the cortical abnormalities associated with autism spectrum disorder overlapped with brain changes associated with genetic vulnerability for autism spectrum disorder in healthy individuals. Lastly, we observed that white matter connectivity between the frontal and parietal regions showed significant association with polygenic risk for autism spectrum disorder, indicating that not only the brain structure, but the white matter connectivity might also show a predisposition for the risk of autism spectrum disorder. Our findings showed that the fronto-parietal thickness and connectivity are dimensionally related to genetic risk for autism spectrum disorder in general population and are also part of the cortical abnormalities associated with autism spectrum disorder. This highlights the necessity of considering continuum models in studying the aetiology of autism spectrum disorder using polygenic risk scores and multimodal neuroimaging.
The Immunoglobulin M-Shed Acute Phase Antigen (SAPA)-test for the Early Diagnosis of Congenital Chagas Disease in the Time of the Elimination Goal of Mother-to-Child Transmission
Authors: Castro-Sesquen Yagahira E , Tinajeros Freddy , Bern Caryn , Galdos-Cardenas Gerson , Malaga Edith S , Valencia Ayala Edward , Hjerrild Kathryn , Clipman Steven J , Lescano Andrés G , Bayangos Tabitha , Castillo Walter , Menduiña María Carmen , Talaat Kawsar R , Gilman Robert H ,   Chagas Working Group in Bolivia and Peru
Published: July 2020

Abstract

Background
Diagnosis of congenital Chagas disease (CChD) in most endemic areas is based on low-sensitive microscopy at birth and 9-month immunoglobulin G (IgG), which has poor adherence. We aim to evaluate the accuracy of the Immunoglobulin M (IgM)-Shed Acute Phase Antigen (SAPA) test in the diagnosis of CChD at birth.

Methods
Two cohort studies (training and validation cohorts) were conducted in 3 hospitals in the department of Santa Cruz, Bolivia. Pregnant women were screened for Chagas disease, and all infants born to seropositive mothers were followed for up to 9 months to diagnose CChD. A composite reference standard was used to determine congenital infection and was based on the parallel use of microscopy, quantitative polymerase chain reaction (qPCR), and IgM–trypomastigote excreted-secreted antigen (TESA) blot at birth and/or 1 month, and/or the detection of anti–Trypanosoma cruzi IgG at 6 or 9 months. The diagnostic accuracy of the IgM-SAPA test was calculated at birth against the composite reference standard.

Results
Adherence to the 6- or 9-month follow-up ranged from 25.3% to 59.7%. Most cases of CChD (training and validation cohort: 76.5% and 83.7%, respectively) were detected during the first month of life using the combination of microscopy, qPCR, and/or IgM-TESA blot. Results from the validation cohort showed that when only 1 infant sample obtained at birth was evaluated, the qPCR and the IgM-SAPA test have similar accuracy (sensitivity: range, 79.1%–97.1% and 76.7%–94.3%, respectively, and specificity: 99.5% and 92.6%, respectively).

Conclusions
The IgM-SAPA test has the potential to be implemented as an early diagnostic tool in areas that currently rely only on microscopy.
Novel electroanatomical map for permanent his bundle pacing: the Mont Blanc approach - influence of the learning curve and procedural outcome
Authors: Imnadze Guram , Vijayaraman Pugazhendhi , Bante Hendrik , Eitz Thomas , Bergau Leonard , Baridwan Nafilah , El Hamriti Mustapha , Molatta Stephan , Braun Martin , Khalaph Moneeb , Sohns Christian , Sommer Philipp 
Published: July 2020

Abstract

Aims
Pacing the specific conduction system like the Bundle of His (HB) can lead to more physiologic activation patterns compared to traditional right ventricular apical pacing. The aim of this study was to estimate the feasibility and value of electroanatomical mapping (EAM) for HB pacing during the learning curve and its impact on procedural outcome.

Methods and results
Fifteen consecutive patients were treated using EAM of the His bundle region before implantation. Voltage and activation maps of HB potentials were performed. The activation time from His potential to R wave (ECG-reference) was measured and correlated to the HV interval. The atrial and ventricular potentials were blended so the active window could only see the His potential. After completing the activation map, it was transformed into a peak-to-peak voltage map of the HB. With reversed black and white colour scale, the exact point of the maximal His signal amplitude was visualized. Procedural data for the implantation were analysed using this innovative approach. The average total procedural time and fluoroscopy time was 88.2 ± 19.1 min and 10.9 ± 4.5 min, respectively. The 3D mapping time was 18.4 ± 5.1 min. The 13.9 ± 5.1 His potential points were needed in average to complete the map. No periprocedural complications were seen in this cohort. In 86.7% of cases, His bundle pacing was successful. The average threshold for the His bundle stimulation and the R-wave amplitude was 1.62 ± 1 V (@1.0 ms) and 4.8 ± 3.2 mV, respectively. The pacing impedance was 513.5 ± 102.8 Ω. Average paced QRS complex width was 116.9 ± 20.3ms. On average 2.6 ± 1.6 lead positions were targeted to find the optimal pacing site.

Conclusion
Electroanatomical mapping-guided implantation of His-bundle leads can facilitate the identification of optimal pacing sites and allow to minimize procedure and fluoroscopy times even during the phase of the learning curve.
The impact of a personalized, community-based counselling and referral programme on modern contraceptive use in urban Ghana: a retrospective evaluation
Authors: Henry Elizabeth G , Hackett Kristy M , Bawah Ayaga , Asuming Patrick O , Agula Caesar , Canning David , Shah Iqbal 
Published: July 2020

Abstract
Community-based demand-generation family planning programmes have been associated with increased contraceptive use in rural areas of Ghana. However, rigorous evaluations of such programmes in urban contexts are lacking. We used a retrospective, cross-sectional with comparison group design to estimate the immediate and sustained impact of the Willows intervention on modern contraceptive use in Kumasi, Ghana. The Willows intervention is a home-based counselling and referral programme for women in low-income urban settlements. We analysed data from a cross-sectional representative survey of 1205 women of reproductive age in the intervention area and 1108 women in a matched comparison site. The main outcome was women’s reported contraceptive use at: (1) baseline (January 2013); (2) programme close (December 2016); and (3) follow-up (August to October 2018). We estimated the programme effect at the community level and for women who reported receiving a family planning counselling visit. We used coarsened exact matching to assess the impact of the intervention relative to outcomes for matched comparison women. Comparing those who reported a family planning visit in the intervention area with matched comparison area women who reported no visit, we estimated a 10.5 percentage point increase in use of modern contraceptives from baseline to close (95%CI : 6.2, 14.8; P &lt; 0.001) and a 7.6 percentage point increase from baseline to follow-up (95%CI : 3.3, 11.9; P &lt; 0.001). However, only 20.2% of women in the Willows intervention area reported a visit. The Willows intervention, therefore, did not achieve its aim to reach all reproductive-aged women in the community. At the community level, we found no significant effect of the intervention at either programme close or 2 years later. We recommend that similar community-based interventions strive for greater outreach and simultaneously launch robust prospective impact evaluations.
The trade-off between biodiversity and sustainable fish harvest with area-based management
Authors: Hilborn Ray , Akselrud Caitlin Allen , Peterson Henry , Whitehouse George A 
Published: July 2020

Abstract
While fisheries provide food and employment for hundreds of millions of people, they also can have significant impact on biodiversity. We explore the potential of area-based fisheries management to simultaneously maintain biodiversity and high levels of sustainable food production. We used two illustrative examples of fisheries that have different gear types, areas, and species to evaluate the trade-off between biodiversity and harvest. We calculate the optimal effort by gear and area that maximizes a weighted objective function of biodiversity and harvest, ranging from 100% of the weight on harvest to 100% on biodiversity. We found for both case studies that the trade-off was highly convex, with win–win solutions allowing for high levels of both fishery harvest and conservation. This is achieved by reducing or eliminating fishing effort that negatively impacts high conservation value species while maintaining fishing effort with gears and in areas where there is low conservation impact. We suggest that, in most fisheries, such situations can be found and that effective area-based management can provide for high levels of biodiversity protection and food production.
Symptoms, Infection Duration, and Hemagglutinin Inhibition Antibody Response in Influenza A Infections
Authors: Tricoche Alexandria D , Wagner Abram L , Balmaseda Angel , Sanchez Nery , Patel Mayuri , Lopez Roger , Schiller Amy , Ojeda Sergio , Frutos Aaron M , Kuan Guillermina , Gordon Aubree 
Published: July 2020

Abstract

Background
Many influenza studies assume that symptomatic and asymptomatic cases have equivalent antibody responses.

Methods
This study examines the relationship between influenza symptoms and serological response. Influenza-positive index cases and household members in Managua, Nicaragua, during 2012–2017 were categorized by symptom status.

Results
Antibody response was assessed using hemagglutination inhibition assays (HAI). Among 510 cases, 74.5% had ≥4-fold increase in HAI antibodies, and 75.3% had febrile illness. In a logistic regression model, febrile cases had 2.17 times higher odds of a ≥4-fold titer rise compared to asymptomatic cases (95% confidence interval, 1.02–4.64).

Conclusions
Studies relying on serological assays may not generalize to asymptomatic infections.
Sleep Problems and Posttraumatic Stress: Children Exposed to a Natural Disaster
Authors: Lai Betty S , La Greca Annette M , Colgan Courtney A , Herge Whitney , Chan Sherilynn , Medzhitova Julia , Short Mary , Auslander Beth 
Published: July 2020

AbstractObjectiveSleep plays a critical role in children’s growth and development. This study examined the frequency and persistence of children’s sleep problems following a natural disaster, risk factors for children’s sleep problems, and the bidirectional relationship between children’s sleep problems and posttraumatic stress symptoms (PTSS) over time.MethodsThis study assessed 269 children (53% female, M = 8.70 years, SD = 0.95) exposed to Hurricane Ike at 8 months (Time 1) and 15 months (Time 2) post-disaster. Children completed measures of hurricane exposure and related stressors, stressful life events, sleep problems, and PTSS.ResultsChildren’s sleep problems were significantly correlated from Time 1 to Time 2 (r = .28, p &lt; .001). Risk factors for sleep problems at Time 2 were younger age, sleep problems at Time 1, and PTSS, not including sleep items, at Time 1. Examinations of the bidirectional relationship between sleep problems and PTSS indicated that PTSS significantly predicted later sleep problems, but sleep problems did not significantly predict later PTSS.ConclusionsFindings demonstrate that PTSS may contribute to the development and course of children’s sleep problems post-disaster.
Multiwavelength analysis of the variability of the blazar 3C 273
Authors: Fernandes S , Patiño-Álvarez V M , Chavushyan V , Schlegel E M , Valdés J R 
Published: July 2020

ABSTRACT
We present multiwavelength light curves and polarimetric data of the Flat Spectrum Radio Quasar 3C 273 over 8 yr. The wavelength range of our data set extends from radio to gamma-rays. We found that the optical emission in this source is dominated by the accretion disc during the entire time-frame of study. We additionally find that in contrast with the observed behaviour in other blazars, 3C 273 does not show a correlation between the gamma-ray spectral index and the gamma-ray luminosity. Finally, we identified an anticorrelation between the 15 GHz and V-band light curves for the time-range JD245 = 4860–5760, which we speculate is the consequence of the inner part of the accretion disc falling into the black hole, followed by the ejection of a component into the jet.
Dust size and spatial distributions in debris discs: predictions for exozodiacal dust dragged in from an exo-Kuiper belt
Authors: Rigley Jessica K , Wyatt Mark C 
Published: July 2020

ABSTRACT
The spectral energy distributions of some nearby stars show mid-infrared (IR) excesses from warm habitable zone dust, known as exozodiacal dust. This dust may originate in collisions in a planetesimal belt before being dragged inwards. This paper presents an analytical model for the size distribution of particles at different radial locations in such a scenario, considering evolution due to destructive collisions and Poynting–Robertson (P–R) drag. Results from more accurate but computationally expensive numerical simulations of this process are used to validate the model and fit its free parameters. The model predicts 11 μm excesses (R11) for discs with a range of dust masses and planetesimal belt radii using realistic grain properties. We show that P–R drag should produce exozodiacal dust levels detectable with the Large Binocular Telescope Interferometer (LBTI) ($R_{11} \gt 0.1{{\ \rm per\ cent}}$) in systems with known outer belts; non-detection may indicate dust depletion, e.g. by an intervening planet. We also find that LBTI could detect exozodiacal dust dragged in from a belt too faint to detect at far-IR wavelengths, with fractional luminosity f ∼ 10−7 and radius ∼10–80 au. Application to systems observed with LBTI shows that P–R drag can likely explain most (5/9) of the exozodiacal dust detections in systems with known outer belts; two systems (β Uma and η Corvi) with bright exozodi may be due to exocomets. We suggest that the three systems with exozodiacal dust detections but no known belt may have cold planetesimal belts too faint to be detectable in the far-IR. Even systems without outer belt detections could have exozodiacal dust levels $R_{11} \gt 0.04{{\ \rm per\ cent}}$ which are problematic for exo-Earth imaging.
Evidence for a high mutual inclination between the cold Jupiter and transiting super Earth orbiting π Men
Authors: Xuan Jerry W , Wyatt Mark C 
Published: July 2020

ABSTRACT
π Men hosts a transiting super Earth (P ≈ 6.27 d, m ≈ 4.82 M⊕, R ≈ 2.04 R⊕) discovered by TESS and a cold Jupiter (P ≈ 2093 d, msin I ≈ 10.02 MJup, e ≈ 0.64) discovered from radial velocity. We use Gaia DR2 and Hipparcos astrometry to derive the star’s velocity caused by the orbiting planets and constrain the cold Jupiter’s sky-projected inclination (Ib = 41°−65°). From this, we derive the mutual inclination (ΔI) between the two planets, and find that 49° &lt; ΔI &lt; 131° (1σ) and 28° &lt; ΔI &lt; 152° (2σ). We examine the dynamics of the system using N-body simulations, and find that potentially large oscillations in the super Earth’s eccentricity and inclination are suppressed by general relativistic precession. However, nodal precession of the inner orbit around the invariable plane causes the super Earth to only transit between 7 and 22 per cent of the time, and to usually be observed as misaligned with the stellar spin axis. We repeat our analysis for HAT-P-11, finding a large ΔI between its close-in Neptune and cold Jupiter and similar dynamics. π Men and HAT-P-11 are prime examples of systems where dynamically hot outer planets excite their inner planets, with the effects of increasing planet eccentricities, planet–star misalignments, and potentially reducing the transit multiplicity. Formation of such systems likely involves scattering between multiple giant planets or misaligned protoplanetary discs. Future imaging of the faint debris disc in π Men and precise constraints on its stellar spin orientation would provide strong tests for these formation scenarios.
Survey of planetesimal belts with ALMA: gas detected around the Sun-like star HD 129590
Authors: Kral Quentin , Matrà Luca , Kennedy Grant M , Marino Sebastian , Wyatt Mark C 
Published: July 2020

ABSTRACT
Gas detection around main-sequence stars is becoming more common with around 20 systems showing the presence of CO. However, more detections are needed, especially around later spectral type stars to better understand the origin of this gas and refine our models. To do so, we carried out a survey of 10 stars with predicted high likelihoods of secondary CO detection using ALMA in band 6. We looked for continuum emission of mm-dust as well as gas emission (CO and CN transitions). The continuum emission was detected in 9/10 systems for which we derived the discs’ dust masses and geometrical properties, providing the first mm-wave detection of the disc around HD 106906, the first mm-wave radius for HD 114082, 117214, HD 15745, HD 191089, and the first radius at all for HD 121191. A crucial finding of our paper is that we detect CO for the first time around the young 10–16 Myr old G1V star HD 129590, similar to our early Sun. The gas seems colocated with its planetesimal belt and its total mass is likely in the range of (2–10) × 10−5 M⊕. This first gas detection around a G-type main-sequence star raises questions as to whether gas may have been released in the Solar system as well in its youth, which could potentially have affected planet formation. We also detected CO gas around HD 121191 at a higher signal-to-noise ratio than previously and find that the CO lies much closer-in than the planetesimals in the system, which could be evidence for the previously suspected CO viscous spreading owing to shielding preventing its photodissociation. Finally, we make estimates for the CO content in planetesimals and the HCN/CO outgassing rate (from CN upper limits), which we find are below the level seen in Solar system comets in some systems.
Frequent Retroviral Gene Co-option during the Evolution of Vertebrates
Authors: Wang Jianhua , Han Guan-Zhu 
Published: July 2020

Abstract
Endogenous retroviruses are ubiquitous in the vertebrate genomes. On occasion, hosts recruited retroviral genes to mediate their own biological functions, a process formally known as co-option or exaptation. Much remains unknown about the extent of retroviral gene co-option in vertebrates, although more than ten retroviral gene co-option events have been documented. Here, we use a phylogenomic approach to analyze more than 700 vertebrate genomes to uncover retroviral gene co-option taking place during the evolution of vertebrates. We identify a total of 177 independent retroviral gene co-option events in vertebrates, a majority of which have not been reported previously. Among these retroviral gene co-option events, 93 and 84 involve gag and env genes, respectively. More than 78.0% (138 out of 177) of retroviral gene co-option occurred within mammals. The gag and env co-option events share a generally similar temporal pattern with less frequent retroviral gene co-option identified in the deep branches, suggesting that retroviral gene co-option might have not been maintained for very long time periods. Moreover, we find co-opted retroviral genes are subject to different selection pressure, implying potentially diverse cellular functionality. Our study provides a comprehensive picture of co-opted retroviral genes during the evolution of vertebrates and has implications in understanding the ancient evolution of vertebrate–retrovirus interaction.