bluuebunny/crossref_metadata_2025_split huggingface dataset:
CRKL but not CRKII Inhibits Erythropoiesis and Megakaryopoiesis of CML via Inactivating Raf/MEK/ERK/Elk-1 Pathway
Authors: Guo Chunmei , Zhang Qiuling , Yan Jinsong , Lv Xinxin , Greenaway Frederick T , Zheng Shanliang , Sun Ming-Zhong , Liu Shuqing 
Published: August 2020

Abstract
Background: As members of the CT10 regulation of kinase (CRK) adaptor protein family, CRK-like (CRKL) and CRKII are involved in cell proliferation, survival, adhesion, migration and differentiation. However, the exact role and underlying mechanism of CRKL and CRKII in leukemic cell differentiation are still unknown. Methods: Quantitative real-time qPCR (qRT-PCR) was used to detect the expression levels of CRKL and CRKII in chronic myeloid leukemia (CML) patients and complete remission (CR) patients; Western blotting (WB) was used to measure the expression levels of CRKL and CRKII during hemin-induced erythroid differentiation of K562 cells; Benzidine staining, isobaric tags for relative and absolute quantitation (iTRAQ) proteomic analysis, cDNA microarray assay, qRT-PCR and WB were used to examine the effects of CRKL and CRKII deregulation on erythroid and megakaryocyte differentiation of K562 cells; PD98059 was used to investigate the underlying mechanism of CRKL in erythropoiesis and megakaryopoiesis. Results: CRKL was found to be overexpressed in chronic myeloid leukemia (CML) patients compared with normal samples, while its expression level was lower in CR patients than in corresponding CML patients. The CRKL expression level was significantly decreased during the erythroid differentiation of K562 cells following hemin treatment. Moreover, CRKL downregulation promoted erythroid and megakaryocyte differentiation of K562 cells accompanied by increased expression level of the erythroid differentiation markers γ-globin, glycophorin (GPA) and the megakaryocyte differentiation markers CD41, CD61. Furthermore, gene microarray and iTRAQ quantitative proteomic analysis showed that CRKL downregulation increased hemoglobin (HB) molecules HBD, HBA1, HBA2, HBZ, HBE1, HBG1 and globin transcription factor 1 (GATA1), high-mobility group protein (HMGB2) expression levels. Mechanistically, CRKL inhibited erythroid and megakaryocyte differentiation of K562 cell via inactivating Raf/MEK/ERK/Elk-1 pathway. Conversely, CRKII was only slightly overexpressed in CML patients and had no effect on erythroid differentiation of K562 cells. Conclusions: Taken together, our results demonstrate that CRKL but not CRKII contributes to development, progression, erythropoiesis and megakaryopoiesis of CML, providing novel insights into effective diagnosis and therapy for CML patients.
Managing Sacral Giant Cell Tumor with Surgery and Various Adjuvant Therapies
Authors: Zhang Yushen , Fu Jun , Xue Yingsen , Guo Zheng , Wang Zhen , Sun Liguo , Liu Xincheng , Fan Hongbin 
Published: August 2020

Abstract
Background and purpose: There is no consensus regarding the appropriate treatment of sacral giant cell tumor (SGCT). This study is to compare oncological and neurological outcomes of SGCT managed by surgery and various adjunctive therapies.Methods: A total of 31 patients with SGCT were retrospectively studied. They were divided into two Groups. A: 13 patients underwent surgery plus SAE and radiotherapy; Group B: 18 patients underwent surgery plus one arterial embolization and denosumab. The postoperative functional outcomes, recurrence, mortality, and complications were compared.Results: The mean operation time (231±49min) and blood loss (3167±856mL) of group B were significantly less than those of group A (283±41min, 5054±689mL) (p<0.05). The average follow-up was 68.5 months. The neurologic function scores showed no significant difference. The recurrence rate of group B (11.2%) was much lower than that of group A (30.7%, p=0.17). The cumulative survival rate of group B was higher than that of group A (p=0.133).Conclusions: In comparison to other adjuvant therapies, one arterial embolization plus denosumab can reduce the intra-operative blood loss, shorten the operation time, and decrease the recurrence rate in patients with SGCT.
Accuracy of Combining End-Tidal Carbon Dioxide and Pulse Pressure Variability Measurements in Predicting Fluid Responsiveness During Passive Leg Raising Test in Septic Shock Patient
Authors: Zhou Xiao-Fen , Yu Rong-Guo , Lin Feng-Hui , Huang Ting-Feng , Gong Shu-Rong , Zhang Ying-Rui , Chen Qian , Chen Han 
Published: August 2020

Abstract
Background

It is essential to assess the patients’ volume responsiveness before fluid infusion in patients with circulatory failure. The hypothesis is that the combining of end-tidal carbon dioxide (EtCO2) and pulse pressure variability (PPV) measurements can non-invasively predict fluid responsiveness during passive leg raising (PLR) test.
Methods

Pulse indicates continuous cardiac output, right radial artery blood pressure, and EtCO2 were monitored in 71 septic shock patients with mechanical ventilation. A standard PLR test was performed; cardiac index (CI), arterial pressure, stroke volume variability (SVV), PPV, and EtCO2 were measured before and after the PLR test. Patients with an increase in CI greater than 15% after the PLR test were defined as fluid responders. Receiver-operating characteristics (ROC) curve analysis was carried out to assess the predictive performance of the measured parameters.
Results

PPV, △EtCO2 and △EtCO2 variation ratio (△EtCO2%) in fluid-responders were significantly greater than those in the non-responders. The AUC of △EtCO2% was 0.884 (95% CI, 0.785 to 0.948) with a cut-off value of > 5.88%; yielded a sensitivity of 91.89% (95% CI, 78.1–98.3%) and aspecificity of 76.47% (95% CI, 58.8–89.3%). The AUC of PPV was 0.852 (95% CI, 0.748 to 0.925) with a cut-off value of > 10%; yielded a sensitivity of 81.1% (95% CI, 64.8–92%) and specificity of 79.41% (95% CI, 62.1–91.3%). The AUC of PPV-△EtCO2% combination was 0.942 (95% CI, 0.86 to 0.984) with a cut-off value of > 0.666; yielded a sensitivity of 83.8% (95% CI, 68–93.8%) and aspecificity of 94.1% (95% CI, 80.3–99.3%). The AUC of PPV-△EtCO2% combination was significantly greater than either △EtCO2% or PPV.
Conclusion

The combination of PPV and △EtCO2 can better predict fluid responsiveness during PLR.
Immune interaction map of human SARS-CoV-2 target proteins: implications for therapeutic avenues
Authors: Subbarayan Karthikeyan , Ulagappan Kamatchi , Wickenhauser Claudia , Seliger Barbara 
Published: August 2020

Abstract
Background There exists increasing evidence that people with preceding medical conditions, such as asthma, diabetes, cancers and heart disease, have a higher risk of infection with SARS-CoV-2 and are more vulnerable to severe disease.Methods To get insights into the role of the immune system upon COVID-19 infection, 2811 genes of the gene ontology term “immune system process GO: 0002376” were selected for analyses. The immune system genes potentially co-expressed with the human targets of SARS-CoV-2 (HT-SARS-CoV-2) ACE2, TMPRSS2 and FURIN were determined in tissue samples from patients with cancer and diabetes mellitus. The network between HT-SARS-CoV-2 and immune system process genes was analyzed based on functional protein associations using STRING. In addition, STITCH was employed to determine druggable targets.Results DPP4 was the only immune system process gene, which was coexpressed with the three HT-SARS-CoV-2 genes, while eight other immune genes were at least co-expressed with two HT-SARS-CoV-2 genes. STRING analysis between immune and HT-SARS-CoV-2 genes plotted 19 associations of 8 commonly networking genes in mixed healthy (323) and cancer (11003) tissues in addition to normal (87), cancer (90) and diabetic (128) pancreatic tissues. Using this approach, three druggable connections between HT-SARS-CoV-2 and immune system process genes were identified. They include positive associations of ACE2 - DPP4 and TMPRSS2 – SRC as well as a negative association of FURIN with ADAM17. Furthermore, the 16 drugs were extracted from STITCH (score <0.8) with 32 target genes.Conclusions This bioinformatics pipeline identified for the first time an immunological network associated with COVID-19 infection thereby postulating novel therapeutic opportunities.
Analysis of foot skeletal structure by 3D foot scanner on smartphone
Authors: Yamashita Tomoko , Yamashita Kazuhiko , Sato Mitsuru , Ryotokuji Takeshi , Kawasumi Masashi , Takase Yoshimasa , Hara Shinsuke 
Published: August 2020

Abstract
Background: The influence of foot skeletal structure on hallux valgus (HV) is poorly understood, so its detailed understanding at an early stage is required for prevention. In this study, a system using a 3D foot scanner on smartphone was developed for the purpose of clarifying relationships between skeletal features and the degree of HV risk. Methods: This system analyzes the foot skeletal structure by sending 2D video images recorded on smartphone to a computer or cloud server, where the 3D foot skeleton model is constructed by the 2D images. This system was developed to identify 10 skeletal features that are expected to be associated with hallux valgus. The participants comprised 419 individuals (40–89 years), and they maintained a standing pose with their toes 12 cm apart and heels 8 cm apart during the video recording. The height and weight were additionally measured to calculate BMI.Results: The study results showed that age-based variations in the foot skeletal features were observed slightly for men whereas distinctively for women. It was observed for women that the great toe-first metatarsal head-heel (GFH) angle increases with age, so it suggests that the increase of the GFH angle characterizes the development of HV. The multiple regression analysis reveals that the features determining the GFH angle are the second toe-heel-navicular angle and the axis of the bone distance among the skeletal features related to the midfoot, and the transverse arch length and height among those related to the forefoot. Their adjusted coefficients of determination were 0.52 and 0.48 for men and women, respectively. Conclusion: Using a 3D foot scanner on smartphone, we developed a simple method to access the skeletal structure of the foot related to HV risk. Trial registration: University hospital Medical Information Network- Clinical Trials (UMIN-CTR) with the number: UMIN 000037694. Registration date: 20/08/2019.
Using pH as a single indicator for evaluating/controlling nitritation systems under influence of major operational parameters
Authors: Chen Chongjun , Wen Jingyu , Zhang Min , Adams Mabruk , Ma Ji , Zhu Guoying 
Published: August 2020

Abstract
This study focused on using pH as a single indicator to evaluate/control the performance of the nitritation system under the influence of three major operational parameters, and a total of fifteen batch tests were conducted. Results indicated that there were important interactions among different operational parameters and pH in the nitritation system; it was possible to propose the optimal nitritation operation scheme to compensate for negative changes in operational parameters. The optimal carbon to nitrogen (C/N) ratio was kept at 2.0 to ensure efficient removal of ammonium. The reaction time was the lowest (150 min) with the temperature = 20 °C, C/N = 0, and sludge/water ratio = 1:1. However, the C/N ratio could be adjusted to close to zero by reducing the temperature to about 10 °C, weakening the heterotrophic bacteria, and supplying sufficient biomass. The C/N ratio and sludge/water ratio could also be set at 4.0 and 1:3 respectively to deal with the impact of low temperature and organic matter. Results of this study might be useful to explain the optimal conditions and process control schemes with pH as a single indicator.
From Social Reform to Knowledge Dissemination
Authors: Tan Hua 
Published: August 2020

The translation of non-literary texts, especially science texts, compared to that of literary texts, tends to receive less attention not only from general readers in public, but also from scholars. One phenomenon of such tendency is that non-literary texts are far less retranslated. Different from literary texts, which could have as many as dozens of retranslations, such as the English novel Jane Eyre, which has more than thirty Chinese retranslations, non-literary texts in general have much fewer retranslations, with many of them never retranslated. The reasons for retranslation of non-literary texts differ from those for literary texts. Literary texts are retranslated, as investigated by many researchers, often because of particular consideration of new target reader groups, language, style, aesthetics, commercial interest, and the like; while non-literary texts tend not to be retranslated for that many different purposes, it is commonly agreed that knowledge dissemination is the major motive behind their retranslations.
Peran WTO dalam Menjembatani Benturan Kepentingan Antara Uni Eropa dan Indonesia dalam Perdagangan Biodiesel
Authors: Mariatulkubtia Anggi 
Published: August 2020

Indonesia's success at the World Trade Organization (WTO) in demanding the European Union (EU) to drop its biodiesel anti-dumping policy in 2018, as well as EU’s compliance with the decision, is a unique case when faced with the perception that international institutions tend to be inclined towards developed countries and disadvantaging developing countries. Utilizing Robert O. Keohane's liberal institutional functionalism theory, this paper describes how the WTO acted as a facilitator in resolving biodiesel disputes between the two parties. This paper argues that the WTO not only provides a dispute settlement mechanism and helps balance information biases, but also raises the cost of reputation and credibility that EU must pay if it chooses to defect the ruling. This research is not intended to show that the WTO, or international institutions and regimes in general, is an antidote for any disputes between developed and developing countries, or that it can always successfully mediate disputes between countries in general. Instead, this paper shows that international institutions can provide a platform for developing countries when facing developed countries, as long as the said actor acts rationally and makes careful calculations about its bargaining position and possible steps to be taken by its opponent.Keywords: WTO, European Union, Indonesia, anti dumping, liberal institutions, international regime, biodiesel. Kemenangan Indonesia di World Trade Organization (WTO) terhadap tuntutan penghapusan bea masuk anti dumping biodiesel Uni Eropa pada tahun 2018, serta kepatuhan Uni Eropa pada keputusan WTO tersebut, dapat dikatakan sebagai sebuah anomali ketika dihadapkan pada persepsi bahwa institusi internasional cenderung condong kepada negara maju dan merugikan negara berkembang. Tulisan ini menjabarkan bagaimana WTO berperan sebagai fasilitator dalam upaya penyelesaian sengketa biodiesel di antara kedua pihak, dengan menggunakan teori fungsionalisme institusi liberal Robert O. Keohane. WTO tidak hanya memberikan platform dan menyeimbangkan bias informasi lewat mekanisme penyelesaian sengketanya, namun juga menaikkan biaya reputasi dan kredibilitas yang harus dibayarkan Uni Eropa jika ia mengabaikan putusan institusi perdagangan internasional tersebut. Penelitian ini tidaklah ditujukan untuk menunjukkan bahwa WTO, atau institusi dan rezim internasional secara umum, dapat menjadi penawar bagi tiap-tiap pertikaian antara negara maju dan negara berkembang, atau menengahi pertikaian antar negara secara umum. Tulisan ini menunjukkan bahwa institusi internasional dapat memberikan wadah bagi negara berkembang ketika dihadapkan dengan negara maju, selama aktor bertindak rasional dan membuat perhitungan yang matang mengenai posisi tawarnya serta langkah-langkah yang akan ditempuh oleh lawannya.Keyword: WTO, Uni Eropa, Indonesia, anti dumping, institusi liberal, rezim internasional, biodiesel.
PENGARUH HERBAFARM DAN PUPUK NPK MUTIARA 16:16:16 TERHADAP PERTUMBUHAN DAN PRODUKSI TANAMAN KACANG HIJAU (Vignaradiata L.)
Authors: Rahmayani Pulungan  , Maizar  , M. Nur  
Published: August 2020

ABSTRACT
The purpose of this study was to determine the interaction and main effects of herbafarm and NPK Mutiara 16:16:16 fertilizer on the growth and production of mung bean plants. (H) consisting of 4 levels namely 0, 1.5, 3, 4.5 cc / L of water, and the second factor is NPK Mutiara 16:16:16 (N) consisting of 4 levels of treatment namely 0, 10.8, 21.6,32.4 grams/plot, so that there are 16 treatment combinations with 3 replications there are 48 experimental unit plots. Each experimental unit consisted of 12 plants and 2 of them were used as sample plants so that the entire plant was 576 plants. The best treatment was giving herbafarm 3 cc / l water and NPK fertilizer pearl 21.6 g / plot ((H2N2) with the results the best filling speed of dry matter is 0.33 g, the percentage of planted pods is 71.33%, the weight of dry seeds is 27.50 grams per harvest. The main effect of giving herbafarm 3 cc / l water gives the best results on plant height 65.67 cm, age flowering 31.92 days, effective filling time 19.74 days and weighing 100 seeds 5.99 grams, while applying NPK pearl fertilizer 21.6 g / plot gives the best results on plant height 61.67 cm and effective filling time 19.90 days, but the application of pearl NPK fertilizer 32.4 g / plot gave the best results at the age of flowering 32.26 days and weighing 100 seeds 5.96 grams.
Keywords: Herbafarm, Growth, Production, Mung Beans.
PENGARUH KEPEKATAN NUTRISI DAN BERBAGAI MEDIA TANAM PADA PERTUMBUHAN SERTA PRODUKSI BAWANG MERAH (Allium ascalonicum L) DENGAN HIDROPONIK NFT
Authors: Shamora Della Hoya Simbolon  , Ernita  , M.Nur  
Published: August 2020

ABSTRACT
The purpose of this study was to investigate the interaction of the application of AB Mix nutrition and growing media on the growth and production of Shallots (Allium ascalonicum L.) on NFT hydroponics. This study uses a ]Compound Plot Design in the form of a Completely Randomized Design consisting of the main plot, namely the nutrient concentration of AB Mix (N) and subplots of planting media (M). Nutrition AB Mix consists of 4 treatment levels, 600, 1200, 1800, 2400 ppm / l water and various planting media treatments consisting of 3 levels, namely husk, cocopeat, and sawdust charcoal media. Each experimental unit consisted of 4 plants and 2 plants used as samples. The parameters observed were as follows: plant height, harvest age, number of tubers, wet tuber weight, dry tuber weight, tuber weight per tuber, tuber weight loss. The observations of each treatment were statistically analyzed and continued with further tests of Honest Significant Difference at the 5% level. The interaction of the application of AB Mix nutrition and planting media had a real effect on all parameters, with the best AB Mix 1800 ppm and cocopeat growing media. The effect of AB Mix main plot has significantly affected all parameters observed with the best treatment of AB Mix 1800 ppm nutrition. The effect of subplot planting media significantly affected all parameters of observation with the best treatment of cocopeat growing media
Keywords: AB Mix, Shallots, NFT hydroponics
Efektifitas Spiritual Hipnoterapi terhadap Penurunan Nyeri Dismenore pada Mahasiswi Kebidanan
Authors: Pratiwi Iva Gamar Dian , Hasanah Laylatul 
Published: August 2020

Dismenore atau nyeri haid yaitu ketidaknyamanan yang sering dialami remaja khususnya perempuan. Dismenore pada remaja terjadi 60% - 90% dan mengganggu aktivitas sehari hari. (Lestari H. Metusala, J.&Suryanto, 2010). Dari hasil penelitian, di Indonesia angka kejadian dismenore sebesar 64,25%, diantaranya 54,89% dismenore primer, 9,36% dismenore sekunder (Ningsih 2011). Wanita membutuhkan obat untuk mengurangi rasa nyeri baik dengan farmakologi maupun non farmakologi. Pengobatan yang di butuhkan wanita yaitu pengobatan yang tidak bersifat invasive dan yang cocok dengan pasien nyeri yang memerlukan penanganan holistik untuk mengintervensi psikis serta mencegah terjadinya konsumsi obat-obatan analgetik yang tidak rasional, pengobatan tersebut yaitu hipnoterapi.( Lucas N.N&Sugianto,2017). Penelitian ini bertujuan untuk menganalisa efektifitas spiritual hipnoterapi terhadap penurunan disminore. Jenis PenelitianÂ ini adalah penelitian kuantitatif pra experimental dengan pendekatan cross sectional. Rancangan penelitian yang digunakan adalah one group pretest posttest, jumlah sampel 30 responden. Tekhnik sampling yang digunakan purposive sampling yang sesuai kriteria inklusi. Penelitian ini dilakukan bulan April sampai Oktober 2019. Intrumen yang digunakan adalah kuesioner penilaian nyeri Visual Analog Scale (VAS) dengan skala Numeric Rating Scale (NRS). Hasil analisa data dengan Wilcoxon signed rank test didapatkan hasil p = 0,000<0,005 maka dapat disimpulkan bahwa spiritual hipnoterapi efektif dalam menurunkan nyeri dismenore pada mahasiswi Kebidanan.Â Dysmenorrhea or menstrual pain is discomfort that is often experienced by adolescents, especially women. Dysmenorrhea in adolescents occurs 60% - 90% and interferes with daily activities. (Lestari H. Metusala, J. & Suryanto, 2010). From the results of the study, in Indonesia the incidence of dysmenorrhea was 64.25%, including 54.89% of primary dysmenorrhea and 9.36% of secondary dysmenorrhea (Ningsih 2011). Women need drugs to reduce the pain, both with pharmacology and non-pharmacology, one of which is hypnotherapy. This study aims to analyze the spiritual effectiveness of hypnotherapy against decreasing dysminorrhea. This type of research is a pre-quantitative quantitative research with cross sectional approach. The research design used was one group pretest posttest, with a sample of 30 respondents. The sampling technique used is purposive sampling that matches the inclusion criteria. This research was conducted from April to October 2019. The instrument used was the Visual Analog Scale (VAS) pain assessment questionnaire with the Numeric Rating Scale (NRS). The results of data analysis with the Wilcoxon signed rank test showed p = 0,000 <0.005, so it can be concluded that there is a meaningful effectiveness of spiritual hypnotherapy for the reduction of dysmenorrhea pain in Midwifery students.