bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of DEGs and transcription factors involved in H. pylori-associated inflammation and their relevance with gastric cancer
Authors: Yin Honghao , Chu Aining , Liu Songyi , Yuan Yuan , Gong Yuehua 
Published: June 2020

Background
Previous studies have indicated that chronic inflammation linked to H. pylori infection is the leading causes for gastric cancer (GC). However, the exact mechanism is not entirely clear until now.

Purpose
To identify the key molecules and TFs involved in H. pylori infection and to provide new insights into H. pylori-associated carcinogenesis and lay the groundwork for the prevention of GC.

Results
GO and KEGG analysis revealed that the DEGs of Hp+-NAG were mainly associated with the immune response, chemokine activity, extracellular region and rheumatoid arthritis pathway. The DEGs of Hp+-AG-IM were related to the apical plasma membrane, intestinal cholesterol absorption, transporter activity and fat digestion and absorption pathway. In Hp+-NAG network, the expression of TNF, CXCL8, MMP9, CXCL9, CXCL1, CCL20, CTLA4, CXCL2, C3, SAA1 and FOXP3, JUN had statistical significance between normal and cancer in TCGA database. In Hp+-AG-IM network the expression of APOA4, GCG, CYP3A4, XPNPEP2 and FOXP3, JUN were statistically different in the comparison of normal and cancer in TCGA database. FOXP3 were negatively associated with overall survival, and the association for JUN was positive.

Conclusion
The current study identified key DEGs and their transcriptional regulatory networks involved in H. pylori-associated NAG, AG-IM and GC and found that patients with higher expressed FOXP3 or lower expressed JUN had shorter overall survival time. Our study provided new directions for inflammation-associated oncogenic transformation involved in H. pylori infection.
Real-time reverse transcription loop-mediated isothermal amplification for rapid detection of SARS-CoV-2
Authors: Lau Yee Ling , Ismail Ilyiana , Mustapa Nur Izati , Lai Meng Yee , Tuan Soh Tuan Suhaila , Hassan Afifah , Peariasamy Kalaiarasu M. , Lee Yee Leng , Chong Yoong Min , Sam I-Ching , Goh Pik Pin 
Published: June 2020

Background
Highly sensitive real-time reverse transcription polymerase chain reaction (RT-qPCR) methods have been developed for the detection of SARS-CoV-2. However, they are costly. Loop-mediated isothermal amplification (LAMP) assay has emerged as a novel alternative isothermal amplification method for the detection of nucleic acid.

Methods
A rapid, sensitive and specific real-time reverse transcription LAMP (RT-LAMP) assay was developed for SARS-CoV-2 detection.

Results
This assay detected one copy/reaction of SARS-CoV-2 RNA in 30 min. Both the clinical sensitivity and specificity of this assay were 100%. The RT-LAMP showed comparable performance with RT-qPCR. Combining simplicity and cost-effectiveness, this assay is therefore recommended for use in resource resource-limited settings.
An Estimate of Variability, Heritability and Genetic Advance for Grain Yield and Yield Components in Bread Wheat (Triticum aestivum L.)
Authors: Jaiswal Ranjana , Gaur S. C. , Jaiswal Sunil K. , Kumar Anil 
Published: June 2020

Problem: Wheat (Triticum aestivum L.) is one of the principal cereal crops grown worldwide and one of the important staples of nearly 2.5 billion of world population. India ranks second position in terms of both in area and production after china. The substantial improvement in production is utmost necessary to feed the growing population.
Objective: In India, wheat requirement by 2030 has been estimated at 100 million metric tons and to achieve this target, wheat production can be increased either through horizontal approach i.e. by the increasing area under cultivation or through vertical approach i.e. varietal/ hybrid improvement. For effective selection and utilization of superior genotype in the germplasm lines, knowledge of genetic parameters such as genetic variability, heritability and genetic advance is essential.
Materials and Methods: The experimental material comprised of ten parents, their 45 F1s and 45 F2s developed by the crossing of 10 parents viz. HUW 510, HUW 234, HUW 468, UP 2338, HD 2402, RAJ 1972, HD 2329, LOK-1, SONALIKA and K 65 in half-diallel programme to study the fourteen characters. The final trial of F1, F2 including parents was conducted during Rabi 2014-15 with three replications employing Randomized Complete Block Design at Research Farm, BRDPG College, Deoria, Uttar Pradesh, India.
Result: Wider range of phenotypic coefficient of variation (PCV) was observed for all the traits in F1 generation ranged from 2.19 (days to 50% flowering) to 13.47 (leaf area index) while, genotypic coefficient of variation (GCV) ranged from 1.50 (days to 50% flowering) to 11.28 (leaf area index). High heritability was observed in both F1 and F2 generation for leaf area index and day to maturity while, moderate to low level of heritability was recorded for other characters. The high value of genetic advance was not recorded in both F1 and F2 generation. Only moderate to the low value of genetic advance was observed for all the fourteen characters under study.
Conclusion: Estimate of phenotypic and genotypic coefficient of variation of fourteen characters in both generations reveals sufficient variability indicating ample scope for genetic improvement of these traits through selection. Moderate level of heritability accompanied with a moderate level of genetic advance was observed for plant height, leaf area index, days to maturity and grain yield per plant in both F1 and F2 generation indicates additive gene effect and selection may be effective.
Chemical Compostions of Essential Oils and Antimicrobial Activity of Hyptis suaveolens (L.) Poit. (Lamiaceae) from Vietnam
Authors: Chung Nguyen Thanh , Huong Le Thi , Dai Do Ngoc , Ogunwande Isiaka Ajani 
Published: June 2020

Aims: This present study described the chemical constituents and antimicrobial activity of essential oils hydrodistiled from the leaves and flowers of Hyptis suaveolens (L.) Poit.
Study Design: This research was designed to accommodate different stages such as collection of authentic sample of Hyptis suaveolens, obtain essential oil by hydrodistillation, chemical analysis of the oil samples by gas chromatography (GC) and gas chromatography coupled with mass spectrometry (GC/MS) and screening of the essential oils for antimicrobial activity.
Place and Duration of Study: School of Natural Science, Vinh University, Vinh City, Nghệ An Province, Vietnam, between August and December 2018.
Methodology: The leaves and flowers of H. suaveolens used for this study were gotten from Đồng Văn Commune, Pù Hoạt Nature Reserve, Vietnam. The collection was done in December 2018. Essential oils were distilled off using hydrodistillation method according to established procedure. The determination of antimicrobial efficacy of essential oils of H. suaveolens will be achieved by the method of microdilution broth susceptibility assay. The minimum inhibitory concentration (MIC) and IC50 were evaluated accordingly.
Results: The major compounds in the oil were b-caryophyllene (31.1% and 33.7%), caryophyllene oxide (17.6% and 3.9%), phytol (9.9% and 2.7%) and a-humulene (6.7% and 6.6%), respectively. The leaf oil displayed strong antimicrobial activity against Enterococcus faecalis ATCC299212, Bacillus cereus ATCC14579 and Candida albicans ATCC10231 with minimum inhibitory concentrations (MIC) of 16.0, 32.0 and 16.0 µg/mL respectively, while the IC50 values were 5.78, 9.35 and 6.78 µg/mL, respectively. The flower oil showed activity towards the same organisms with MIC values of 64.0, 64.0 and 16.0 µg/mL, respectively; with IC50 values of 20.45, 26.78 and 6.78 µg/mL, respectively. Both essential oils exhibited moderate activity towards Staphylococcus aureus ATCC25923 with MIC of 256.0 µg/mL.
Conclusion: The results are indication of the potential of H. suaveolens essential oils as source of antimicrobial agents.
Effect of Caffeine on Contrast Sensitivity among Young Adults of Abia State University
Authors: Obinna Anyatonwu Princewill , Ihesiulor Chikezie Grand , Udo Ubani Ahanna 
Published: June 2020

Introduction: Caffeine is the most widely consumed psychoactive substance. Unlike many other psychoactive substances, it is legal and unregulated in the world. Several experts suggest that moderate use of caffeine (300 mg or equivalent to 3 cups or less to regular size cups of coffee per day) is safe and so not likely to cause health issues. Intake of caffeine has been suggested to affect the visual system. This study examined the effect of consumption of caffeine on contrast sensitivity of the eye among young adults of Abia State University students within the age range of 17-30 yrs.
Methods: This study employed a cross-sectional design and was carried out within the confinement of Abia State University Eye Clinic. A total of 100 young adults consisting of an equal number of males and females were measured using the Rabin’s contrast sensitivity at baseline, 30 mins until 120 mins. One way analysis of variance (ANOVA) and the Chi-square statistical analysis was used to analyze statistically the research hypotheses at 95% level of significance.
Results: The findings of this study reveal that mean contrast sensitivity values for 16 to less than 20 years old were 1.70 at baseline and increased to 1.79, 1.89, 1.93 except at 120 minutes post-ingestion of caffeine. In males and females, contrast sensitivity increased by 1.57%, 3.7%, 5.3% and 10.7%, 20.7% 23.3% respectively. At 95% confidence level, caffeine ingestion had a significant effect on contrast sensitivity for both males (4.79) and females (5.8).
Conclusion: This study depicts that the ingestion of caffeine affects or increases contrast sensitivity in both males and females although this effect is short-lived.
Social media reduce users' moral sensitivity: Online shaming as a possible consequence
Authors: Ge Xiaoyu 
Published: June 2020

AbstractIn this study, we propose that social media reduce users' moral sensitivity through the mediation of the perceived moral intensity of hostile comments, which leads to behavioral consequences for online shaming. Three separate studies were conducted to explore this statement. Study 1 (N = 160) compared moral sensitivity between participants in simulated social media situations and a control group. Study 2 (N = 412) tested the mediating role of perceived moral intensity through self‐rated questionnaires. Study 3 (N = 295) examined the behavioral consequences of reduced moral sensitivity on online shaming by manipulating social media and perceived moral intensity. Across these three studies with their different methodologies, we found consistent support for our prediction that social media reduce users' moral sensitivity. Also, our findings shed light on perceived moral intensity as a mediator. As expected, less perceived moral intensity and less moral sensitivity (as serial mediators) induced by social media led to a higher tendency to participate in online shaming. In addition, our research suggests that the harmful effects of social media could be restricted by improving users' perceived moral intensity in the form of reminders. These findings provide novel insights into the underlying mechanism of cyberviolence on social media and also contribute to the literature on the antecedents and consequences of moral sensitivity.
Impact of a Multifaceted Educational Program to Improve Provider Skills for Lupus Pregnancy Planning and Management: A Mixed‐Methods Approach
Authors: Njagu Ravyn , Criscione‐Schreiber Lisa G. , Eudy Amanda , Snyderman Amanda , Clowse Megan E. B. 
Published: June 2020

ObjectiveTo bring recent advances in pregnancy management in lupus to women nationwide, this multidimensional educational intervention sought to equip community rheumatologists with the needed skills, attitudes, and confidence to manage contraceptive decisions and pregnancy planning for women with lupus.MethodsThe program included an in‐person didactic, training in use of a comprehensive handout to guide contraception and pregnancy conversations, a simulated clinical experience, and access to an innovative website (www.lupuspregnancy.org). The program was analyzed using mixed methods, which included a quantitative survey by e‐mail before and after program completion and multiple qualitative interviews about attendees’ experiences integrating created resources into practice.ResultsThe analysis included 68 preintervention surveys and 55 postintervention surveys. For qualitative analysis, eight interviews were completed until thematic saturation was achieved. After completion of the program, there was an increase in providers reporting a systematic approach to preparing a woman with lupus for pregnancy (from 45.6% to 94.6%; P < 0.0001). Confidence in choosing both appropriate contraception and pregnancy‐compatible medications improved significantly. As expected, change in knowledge about contraception was limited. Qualitative themes included the utility of the printable handouts, enthusiasm for the program, increased confidence and, importantly, increased empathy for the patients.ConclusionWe created a valuable implementation tool that improves self‐reported provider skills and confidence in managing women with lupus who desire pregnancy. Providers now have access to a unique curriculum and resources that encourage providers to have open and accurate conversations about pregnancy, thus creating lasting clinical change.
p‐Type Transparent Quadruple Perovskite Halide Conductors: Fact or Fiction?
Authors: Hu Sanlue , Xia Bing , Lin Yang‐Peng , Katase Takayoshi , Fujioka Jun , Kamiya Toshio , Hosono Hideo , Du Ke‐Zhao , Xiao Zewen 
Published: June 2020

AbstractHigh‐performance p‐type transparent conductors (TCs) can be used for a variety of optoelectronic applications, particularly transparent electronics with complementary metal–oxide–semiconductor circuits. Recently, several quadruple perovskite halides, such as Cs4CdSb2Cl12 and Cs4CdBi2Cl12, have been theoretically predicted to be excellent p‐type TCs that surpass all the conventional p‐type TCs and hence attracted a lot of attention. In this work, experiments and hybrid density functional theory (DFT) calculations are combined to evaluate the p‐type dopability in these quadruple perovskite halides. The experimental results reveal that these materials are optically transparent but electrically insulating, which contradicts the previous prediction. The hybrid DFT calculations show that the difficulty in forming p‐type doping in these compounds is because of the easy formation of the compensating n‐type intrinsic defects in the p‐type region, which is resulted from too deep valence band maximum levels. It is further demonstrated that the bandgap underestimation associated with the semilocal DFT and the unintentional chemical boundary overestimation should be the origins of the p‐type conductivity predicted previously. The results highlight the importance of reasonable bandgap description and chemical boundary determination in predicting defect thermodynamics.
Interconnection between Trait, Structure, and Composition of Grain Boundaries in Cu(In,Ga)Se2 Thin‐Film Solar Cells
Authors: Raghuwanshi Mohit , Wuerz Roland , Cojocaru‐Mirédin Oana 
Published: June 2020

AbstractGrain boundaries (GBs) are crucial for solar cells incorporating polycrystalline absorbers and particularly for those characterized by small grain sizes (≈2 µm). For example, random GBs in Si solar cells are found to have a detrimental effect on the cell performance being characterized by an increased recombination activity relative to grains. Yet, their role in Cu(In,Ga)Se2 (CIGS) solar cells still remains controversial. The recent electron‐beam‐induced current (EBIC) study shows that 58% of the GBs in CIGS exhibit enhanced electrical properties considered to be benign (for the device performance). Yet, they coexist with 16% detrimental GBs (reduced electrical properties) and 27% neutral ones (no change in electrical property when compared with the bulk). In the present study, these different GBs are investigated by combining EBIC with electron backscattered diffraction and atom probe tomography techniques on identical GBs. For the first time, a successful correlation is shown (for any device) that interconnects the GB characteristics to its composition. Sufficient statistics demonstrate that the collective fluctuations of all elements at GBs determine its trait. In general, benign (detrimental) GBs are characterized by Cu depletion (enrichment) that favored the formation of donor (acceptor) defects.
Low Cost, Flexible, Perovskite BaTiO3 Nanofibers‐Based p–n Homojunction for Multifunctional Sensing of Physical and Chemical Stimuli
Authors: Veeralingam Sushmitha , Ravindranath Ajay Narayan Konda , Badhulika Sushmee 
Published: June 2020

AbstractIn this work, a flexible, low cost, and cleanroom‐free technique to fabricate barium titanate (BaTiO3) hybrid p–n homojunction‐based multifunctional sensor for photodetection, strain sensing, and chemiresistive sensing is reported. The n‐type BaTiO3 nanofibers are synthesized using electrospinning technique and deposited on the flexible indium tin oxide (ITO) using spin‐coating method while p‐type BaTiO3 nanoparticles are synthesized using modified sol–gel method and deposited using drop‐casting method. Detailed characterization studies are performed to reveal the formation of tetragonal phase p‐type nanoparticles and n‐type nanofibers of BaTiO3 assembled on the ITO substrate. The fabricated hybrid junction photodetector displays an excellent external quantum efficiency of ≈3500 and photoresponsivity of ≈11 A W−1. Further, the hybrid junction when employed as strain sensor exhibits a gauge factor of 14 and when operated in chemiresistive sensing mode can detect α1‐acid glycoprotein with an excellent sensitivity of 49.7 µm−1 in the wide dynamic range of 0.05–10 × 10–6 m and detection limit of 12 × 10–9 m. Detailed studies to understand the underlying mechanism of operation for each application are done. The strategy outlined here can be further employed for sensing various physical and chemical stimuli, paving a new path for developing flexible multifunctional nanoelectronic devices.
In‐Depth Atomic Mapping of Polarization Switching in a Ferroelectric Field‐Effect Transistor
Authors: Li Xiaoyan , Zhu Qiuxiang , Vistoli Lorenzo , Barthélémy Agnès , Bibes Manuel , Fusil Stéphane , Garcia Vincent , Gloter Alexandre 
Published: June 2020

AbstractThe ferroelectric control of a Mott transistor is a promising strategy for nonvolatile low‐power electronics. Understanding the fundamental limits of the ferroelectric‐field effect is challenging, as the relevant length scales are restricted to a few atomic planes within the interface. Here, the polarization switching process of a prototypical ferroelectric Mott transistor combining BiFeO3, a ferroelectric material with a large polarization, and (Ca,Ce)MnO3, a charge‐transfer insulator in which a few percent of Ce doping triggers a metal–insulator transition is investigated. While scanning probe microscopy indicates a complete switching of the ferroelectric gate, in‐depth atomic‐scale polarization mapping with scanning transmission electron microscopy reveals incomplete polarization reversal at the interface. Therefore, transport measurements show that the electronic properties of the Mott channel are virtually unchanged by the polarization direction. Nevertheless, in nanometer size areas where interfacial polarization switching occurs, dramatic changes of the electronic properties of (Ca,Ce)MnO3 are revealed. These results indicate how the performance of mesoscale Mott devices is hindered, and at the same time reveal the possibility of nanoscale energy‐efficient Mott transistors.
Iridium‐Catalyzed Oxidative Annulation of 2‐Arylindoles with Benzoquinone Leading to Indolo[1,2‐f]phenanthridin‐6‐ols
Authors: Guo Shenghai , Liu Yangfan , Zhang Xinying , Fan Xuesen 
Published: June 2020

AbstractAn iridium‐catalyzed oxidative [4+2] annulation reaction of 2‐arylindoles using quinone derivatives as C2 synthons has been developed. From this reaction, a series of diversely substituted indolo[1,2‐f]phenanthridine derivatives were synthesized in an efficient manner. Moreover, diverse transformations of the phenanthridine derivatives thus obtained have also been performed, which showcase the synthetic potential of the present protocol.magnified image
Accelerated weathering behavior of castor oil bio‐based thermosets
Authors: Echeverri David A. , Pérez William A. , Inciarte Helen C. , Rios Luis A. 
Published: June 2020

AbstractBio‐based thermosets based on vegetable oils are promissory materials due to their good performance, renewability and biodegradability. In this work, accelerated weathering tests were performed over bio‐based thermosets obtained from castor oil to evaluate the long‐term performance under environmental conditions. The thermosets were obtained through homopolymerization or copolymerization with styrene of maleated castor oil methacrylate. The changes in chemical and physical properties were monitored by Fourier‐transform infrared spectroscopy (FTIR), color measurements, scanning electron microscopy (SEM) and proton nuclear magnetic resonance (1HNMR). Both materials showed evidences of chemical changes, such as formation of double bonds and hydroxyl groups, as well as the decomposition of carbonyl ester groups. The observed physical changes were discoloration as well as the formation of cracks and blistering. However, the degradation phenomena were less severe in the copolymer due to the high stability of styrene moieties.
A Five‐layer π‐Aromatic Structure Formed through Self‐assembly of a Porphyrin Trimer and Two Aromatic Guests
Authors: Ueda Masahiro , Kimura Masaki , Miyagawa Shinobu , Takaya Hikaru , Naito Masaya , Tokunaga Yuji 
Published: June 2020

AbstractIn this study we synthesized two‐ and four‐armed porphyrins – bearing two carboxyl and four 2‐aminoquinolino functionalities, respectively, at their meso positions – as a complementary hydrogen bonding pair for the self‐assembly of a D2‐symmetric porphyrin trimer host. Two units of the two‐armed porphyrin and one unit of the four‐armed porphyrin self‐assembled quantitatively into the D2‐symmetric porphyrin trimer, stabilized through ammidinium‐carboxylate salt bridge formation, in CH2Cl2 and CHCl3. The porphyrin trimer host gradually bound two units of 1,3,5‐trinitrobenzene between the pair of porphyrin units, forming a five‐layer aromatic structure. At temperatures below −40 °C, the rates of association and dissociation of the complexes were slow on the NMR spectroscopic time scale, allowing the 1 : 1 and 1 : 2 complexes of the trimer host and trinitrobenzene guest(s) to be detected independently when using less than 2 eq of trinitrobenzene. Vis titration experiments revealed the values of K1 (2.1±0.4×105 M−1) and K2 (2.2±0.06×104 M−1) in CHCl3 at room temperature.