bluuebunny/crossref_metadata_2025_split huggingface dataset:
Lipidomics Profiling of Skin Surface Lipids in Senile Pruritus
Authors: Ma xiaolei , Lu Lulu , Zhao Zheng , Cai Mingru , Gao Na , Han Gangwen 
Published: July 2020

Abstract
Background: Senile pruritus is common, yet its etiology remains unknown. Aging-associated skin barrier defects and skin surface lipid (SSL) alterations have been postulated to play important roles in its occurrence. In the present study, the lipidomic profiles of SSLs in elderly patients were examined to better understand the potential causes of senile pruritus. Methods: Transepidermal water loss (TEWL) was evaluated to assess the skin barrier function. The Ameliorated Kawashima Itch Scale score was used to measure the pruritus severity. Liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) and multivariate data analysis were employed to investigate SSL alterations. Results: The results showed that senile pruritus patients had higher TEWL values than control subjects (13.13±4.28 versus 6.71±2.45, p<0.01). LC-MS/MS revealed significant differences in the lipidomic profiles and identified 81 species of SSLs that differed between the two groups. Compared with control subjects, senile pruritus patients had increased levels of ceramides (Cers), diacylglycerols, fatty acids, phosphatidylcholines, phosphatidylethanolamines, phytosphingosines, sphingosines, diacylceryl-3-O-carboxyhydroxymethylcholine, diacylglyceryl trimethylhomoserine, and unsaturated free fatty acids, but decreased levels of triacylglycerol. Cer-EOS, Cer-NDS, and Cer-NS were positively correlated with TEWL value (p<0.05). Pruritus severity score was positively correlated with sphingomyelin, Cer-NP, Cer-AS, Cer-NDS, and Cer-NS, but negatively correlated with Cer-BS, Cer-EODS, Cer-EOS, and Cer-AP. Conclusions: The present study indicated that patients with senile pruritus have impaired skin barrier function and altered SSL composition. Certain SSL species identified in this study may be potential targets for future studies on the pathogenesis of senile pruritus.Trial registration: Peking University International Hospital (Number: YN2018QN04; date: January 2019).
Changes in Self-Reported Sleep Duration With Age - a 36-Year Longitudinal Study of Finnish Adults
Authors: Hublin Christer , Haasio Lassi , Kaprio Jaakko 
Published: July 2020

Abstract
Background

Sleep deprivation is often claimed to be increasingly common, but most studies show small changes in sleep duration over the last decades. Our aim was to analyze long-term patterns in self-reported sleep duration in a population-based cohort.

Methods

Members of the Older Finnish Twin Cohort have responded to questionnaires in 1975 (N = 30,915 individuals, response rate 89%, mean age 36 years), 1981 (24,506, 84%, 41 years), 1990 (12,502, 77%, 44 years), and 2011 (8510, 72%, 60 years). Weibull regression models were used to model the effects of follow-up time and age simultaneously.

Results

Sleep duration has decreased in all adult age groups and in both genders. The mean duration was in men 7.57 hours in 1975 and 7.39 in 2011, and in women 7.69 and 7.37, respectively. The decrease was about 0.5 minutes in men and 0.9 in women per year of follow-up. In the age-group 18-34 years, mean sleep length was 7.69 hours in 1975 and 7.53 in 1990. Among 35-54-year-old it was 7.57 hours in 1975 and 7.34 in 2011, and in the age group of 55+ year olds 7.52 and 7.38, correspondingly. The change was largest in middle-aged group: about 23 minutes or about 0.6 minutes per year of follow-up.

Conclusions

There has been a slight decrease in mean sleep duration during the 36-year follow-up. Although the sleep duration was longer in 1970s and 1980s, the probable main cause for the change in this study population is the effect of aging.
Novel Transplant of Combined Platelet-rich Fibrin Releasate and Bone Marrow Stem Cells Prevent Bone Loss in Ovariectomized Osteoporotic Mice
Authors: wong chin chean , Liao Jeng-Hao , Sheu Shi-Yuan , Lin Po-Yu , Chen Chih-Hwa , Kuo Tzong-Fu 
Published: July 2020

Abstract
Background: Osteoporosis is a metabolic bone disorder characterized by deterioration in the quantity and quality of bone tissue, with a consequent increase susceptibility to fracture. Methods: In this study, we sought to determine the efficacy of platelet-rich fibrin releasates (PRFr) in augmenting the therapeutic effects of stem cell-based therapy in treating osteoporotic bone disorder. An osteoporosis mouse model was established through bilateral ovariectomy on 12-week-old female ICR (Institute of Cancer Research) mice. Eight weeks postoperatively, the ovariectomized (OVX) mice were left untreated (control) or injected with PRFr, bone marrow stem cells (BMSCs), or the combination of BMSCs and PRFr. Two different injection (single versus quadruple) dosages were tested to investigate the accumulative effects of BMSCS and PRFr on bone quality. Eight weeks after injection, the changes in tibial microstructural profiles included the percentage of bone volume versus total tissue volume (BV/TV, %), bone mineral density (BMD, g/cm3), trabecular number (Tb.N, number/mm), and trabecular separation (Tb.Sp, mm) and bony histology were analyzed. Results: Postmenopausal osteoporosis model was successfully established in OVX mice, evidenced by reduced BMD, decreased BV/TV, lower Tb.N but increased Tb.Sp. Eight weeks after injection, there was no significant change to BMD and bone trabeculae could be detected in mice that received single-injection regimen. In contrast, in mice which received 4 doses of combined PRFr and BMSCs, the BMD, BV/TV, and TB.N increased, and the TB.Sp decreased significantly compared to untreated OVX mice. Moreover, the histological analysis showed the trabecular spacing become narrower in OVX-mice treated with quadruple injection of BMSCs and combined PRFr and BMSCs than untreated control.Conclusion: The systemic administration of combined BMSCs and PRFr protected against OVX-induced bone mass loss in mice. Moreover, the improvement of bony profile scores in quadruple-injection group is better than the single-injection group, probably through the increase in effect size of cells and growth factors. Our data also revealed the combination therapy of BMSCs and PRFr has better effect in enhancing osteogenesis, which may provide insight for the development of a novel therapeutic strategy in osteoporosis treatment.
Awareness, Utilization and Influencing Factors of Social Support for Main Informal Caregivers of Schizophrenia Patients: A Cross-sectional Study In Primary Care Settings In Beijing, China
Authors: Wang Meirong , Jin Guanghui , Wei Yun , Wang Feiyue , Pan Zhaolu , Chen Lifen , Lu Xiaoqin 
Published: July 2020

Abstract
Background: Main informal caregivers of people with schizophrenia in Beijing are increasingly called upon to provide home-based care for their patients due to the increasing number of schizophrenia patients. This study aims toinvestigate the further needs of social supportsin the perspective of the main informal caregivers and to analyze the influencing factors of awareness and utilization of social supports in Beijing. The results obtained from this study could potentially act as reference for health and clinical professionals to implement suitable and effective programs and interventions for main informal caregivers.Methods: A mixed-methods approach were used in this study. We performed individual in-depth interviews with 10main informal caregivers fromurban districts of Beijing to identify thecaregivers’ perspective on social supports and further needs.Meanwhile, the factors influencing the awareness and utilization of social supportsfor main informal caregiverswere assessed utilizing self-designed questionnairein 6 urban districts in Beijing.Results: Most caregiversin in-depth interviews expressed that they had negative experiences with respect to obtaining social support, and they emphasized that they would require more support. More financial support, respect, and rehabilitation institutions were needed by the caregivers.Quantitative results show that although the government provides multi-channel resources forschizophrenia patients and their families,the awareness and utilization rate of some resources such as rehabilitation, employment, and health services were relatively lower, the rate were less than 10.0% and 5.0% separately. Conclusions: The awareness and utilization rate of social support are lowforschizophrenia patients.More services and better public attitudes should be considered for schizophrenia patients and their caregivers.
QTL Mapping and Identification of Candidate Genes for Heat Tolerance at the Flowering Stage in Rice
Authors: Chen Lei , Wang Qiang , Zhang Xiaoli , Tang Maoyan , Pan Yinghua , Gao Guoqing , Lv Ronghua , Tao Wei , Jiang Ligeng , Liang Tianfeng 
Published: July 2020

Abstract
Background: High-temperature stress can cause serious abiotic damage that limits the yield and quality of rice. Heat tolerance during the flowering stage of rice is a key trait that can guarantee a high and stable yield under heat stress. Heat tolerance is a complex trait that is regulated by numerous quantitative trait loci (QTLs); of which, however, few underlying genes have been fine mapped and cloned.Results: In this study, the F2:3 population derived from a cross between Huanghuazhan (HHZ), a heat-tolerant indica cultivar, and 9311, a heat-sensitive indica rice variety, were used to map the QTLs for heat tolerance during the flowering stage of rice. The F2:3 lines were treated under 38 °C from 9:30 am to 3:30 pm continuously for 3 days in a phytotron, and spikelet fertility was assessed. A new major QTL, qHTT8, controlling heat tolerance was located on chromosome 8 using the bulked-segregant analysis (BSA)-seq method. The QTL qHTT8 was determined to be located in the 355500–4520000 bp region, with a size of 0.965 Mb. The candidate region of qHTT8 on chromosome 8 contained 65 predicted genes. Ten putative predicted genes were found to be associated with abiotic stress tolerance. In future studies, the backcross population will be constructed to fine map and validate the effect of qHTT8.Conclusion: The information obtained in this study is useful for the fine mapping and cloning of qHTT8 and breeding heat-tolerant varieties of rice using marker-assisted selection.
PvB3s: Genome-wide and Transcriptome-wide Identification and Analysis Reveals the B3 Family Regulate Auxin to Resist Salt Stress at the Sprout Stage in Common Bean(P. Vulgaris L. ) .
Authors: Zhang Qi , Li Wei-jia , Zhang Wen-jing , Yin Zhen-gong , Wang Yu-xin , Zhang Ming-yu , Zhang Shuo , Liu Yang-cheng , Zhao Hao-hao , Zhang Wen-hui , Du Ji-Dao 
Published: July 2020

Abstract
Background: B3 gene family is a transcription factor family unique to plants, which play an important role in plant growth and development by binding specific DNA sequences. However, data on the B3 genes in the common bean and participate in many abiotic stresses especially salt stress are limited. Result: A total of encoding 100 proteins were identified in common bean. Phylogenetic analysis showed that PvB3s were classified into 4 subgroups, and these clusters were supported by several group-specific features, including exon/intron structure, MEME motifs, and predicted binding site structure. Collinearity analysis showed the connection of PvB3s in the same species and different species. The genes expression pattern showed that PvB3s expressed with a tissue-specific manner during sprout stage. Through RNA-seq and qRT-PCR analysis, it was found that there were differences in expression in extreme materials under salt stress. The determination of auxin content and the analysis of PvB3s expression in the enriched pathway showed that PvB3s would respond to auxin to enhance salt tolerance in common bean sprouting stage. Conclusion: The results provided useful and rich resources of PvB3s for the functional characterization and understanding of B3 transcription factors (TFs) in common bean, which further provides insights that PvB3s may respond to auxin to enhance salt tolerance of common bean.
Influence of Saskatoon Berry and Cyanidin-3-Glucoside on Gut Microbiota and Relationship with Metabolism and Inflammation in High Fat-High Sucrose Diet-Induced Insulin Resistant Mice
Authors: Huang Fei , Zhao Ruozhi , Xia Mi , Shen Garry 
Published: July 2020

Abstract
Background

Type 2 Diabetes (T2D) has become one of most common and harmful chronic diseases worldwide. T2D is characterized as insulin resistant and is often associated with unhealthy dietary habits. The present study assessed the effects of freeze-dried Saskatoon berry powder (SBp) and cyanidin-3-glucoside (C3G, an anthocyanin enriched in SBp) on metabolism, inflammatory markers and gut microbiota in high fat-high sucrose diet (HFHS) diet induced insulin resistant mice.
Results

Male C57 BL/6J mice received control, HFHS, HFHS + SBp (8.0 g/kg body weight/day) or HFHS + C3G (7.2 mg/kg/day, equal amount of C3G in 8.0 g/kg/day SBp) diet for 11 weeks. HFHS diet significantly increased the levels of glucose, cholesterol, triglycerides, insulin resistance and inflammatory mediators in plasma. The results of 16S rRNA gene sequencing demonstrated that HFHS diet increased the ratio of Bacteroidetes/Firmicutes (B/F) phylum bacteria and an elevated abundance of Muriculaceae family bacteria in the feces of mice. SBp or C3G supplementation attenuated HFHS diet-induced disorders in metabolism and inflammatory markers, and increased B/F ratio and Muriculaceae abundance in mouse gut compared to HFHS diet alone. The abundance of Muriculaceae in the gut microbiota negatively correlated with body weight, glucose, lipids, insulin resistance and inflammatory mediators in mice. The results of functional predication analysis suggest that HFHS diet upregulated the genes of gut bacteria involved in inflammation-related cellular processes, and inhibited bacteria involved in metabolism. SBp and C3G partially neutralized the alterations induced by HFHS diet in gut microbiota implicated in metabolism or inflammation.
Conclusion

The findings of the present study suggest that SBp is a potential prebiotic food mitigating Western diet-induced disorders in metabolism, inflammation and gut dysbiosis, and C3G possibly contributes to the beneficial effects of SBp.
Brain HIV-1 latently-infected reservoirs targeted by the suicide gene strategy
Authors: Saeb Sepideh , Ravanshad Mehrdad , Pourkarim Mahmoud Reza , Daouad Fadoua , Baesi Kazem , Rohr Olivier , Wallet Clémentine , Schwartz Christian 
Published: July 2020

Abstract
Several strategies are currently investigated to reduce the pool of all HIV-1 reservoirs in infected patients in order to achieve functional cure. The most prominent HIV-1 cell reservoirs in the brain are microglial cells. Virus infection maybe lifelong. Infected microglial cells are believed to be the source of peripheral tissues reseeding and responsible for the emergence of drug resistance. Clearing infected cells from the brain is therefore crucial. However, many characteristics of microglial cells and the central nervous system prevent the eradication of brain reservoirs. Current trials, such as “shock and kill”, the “deep and lock” and the gene editing strategies do not respond to these difficulties. Therefore, new strategies have to be designed when considering brain reservoirs such as microglial cells. We set up an original gene suicide strategy using a latently infected microglial model. In this paper we provide proof of concept of this strategy. Our results demonstrate that this strategy enables the eradication of latently-infected microglial cells.
The Effects of Race/Ethnicity and Physician Recommendation for Physical Activity on Physical Activity Levels and Arthritis Symptoms Among Adults With Arthritis
Authors: Huckleby Jeremy , Williams Faustine , Ramos Rose , Napoles Anna 
Published: July 2020

Abstract
Background: Among U.S. adults with physician-diagnosed arthritis, we examined the effects of race/ethnicity and receiving physician exercise recommendation on meeting aerobic and strengthening physical activity guidelines, and arthritis symptoms, and whether race/ethnicity moderates the effects of physician recommendation on activity levels and symptoms.Methods: Retrospective, cross-sectional study of National Health Interview Survey pooled data from 2002, 2006, 2009, and 2014. The study included 27,887 U.S. adults aged ≥18 years with arthritis. Outcomes were meeting aerobic (yes/no) and strengthening guidelines (yes/no), arthritis-associated activity limitations (yes/no) and arthritis-related pain (0-10; higher score=more pain). Predictors were race/ethnicity (White, African American, Latino, and Asian) and receipt of physician recommendation for exercise (yes/no). Covariates included demographic and health characteristics and U.S. region.Results: Controlling for covariates, physician exercise recommendation was independently associated with meeting aerobic (AOR=1.14; 95% CI 1.06, 1.24) and strengthening (AOR=1.17; 95% CI 1.06, 1.28) guidelines; effects did not differ by race/ethnicity. African Americans were more likely than Whites to meet strengthening guidelines (AOR=1.22; 95% CI 1.07, 1.40) and Asians were less likely to meet aerobic (AOR=0.80; 95% CI 0.65, 0.99) and strengthening (AOR=0.76; 95% CI 0.60, 0.96) guidelines. Compared to Whites, African Americans (B=0.51; 95% CI 0.26, 0.76) and Latinos (B=0.43; 95% CI 0.14, 0.72) reported more severe, while Asians reported less severe (B=-0.60; 95% CI -1.17, -0.04) joint painConclusions: Disparities in pain exist for African Americans and Latinos with arthritis. Physician exercise recommendation is critical among patients with arthritis to relieve symptom burden.
Demographic and clinical characteristics of foreign residents who visited hospitals for mental health problems in Japan: a multicenter study in a metropolitan area
Authors: Takubo Youji , Nemoto Takahiro , Iwai Momoko , Kashima Minako , Yamaguchi Eriko , Maruyama Akiko , Miura Sachio , Saito Hisaaki , Tsujino Naohisa , Mizuno Masafumi 
Published: July 2020

Abstract
Background:
International immigration to Japan, where homogeneous ethnicity is a population characteristic, has been growing. Although immigration is recognized as a risk factor for multiple mental-health related issues, there are few regional reports on foreign residents who visit a hospital for mental health problems in Japan. We aimed to examine such patients’ characteristics.
Methods:
A multicenter retrospective study using medical records was conducted. The subjects were foreign patients who presented at the psychiatry departments in three core regional hospitals in the Keihin region, which faces Tokyo Bay and is well known to include the largest traditional industrial zone in Japan, over a period of three years. We investigated the patients’ demographic and clinical information including nationality/region, spoken language, use of a medical interpreter, pathway to hospitals, and outcome.
Results:
The percentage of foreign individuals among all patients was 1.4% (205/14511). The mean age of the foreign patients was 45.8 years, and the sex ratio was 1:1.9 (men: women). China (35.1%) was the most common nationality, followed by the Philippines (18.5%), Korea (16.1%), and Brazil (4.9%). About one-fourth of the subjects could not speak Japanese; therefore, interpretation was required by family members/friends (17.1%) or a professional interpreter (5.4%). Neurotic disorder (ICD code: F4) was the most common diagnosis (24.4%).
Conclusions:
The percentage of foreign patients seeking psychiatric treatment (1.4%) was relatively low, compared with the percentage of foreign residents living in the Keihin region (4.4%). The age distribution of foreign residents who visited psychiatric department was dissociated from the age distribution of foreign residents in Japan. This suggests that young foreign residents with a relatively high risk for mental illness are not accessing appropriate services. The development of a community-based integrated care system accessible to foreign residents seems to be indispensable.
CT Blend Signs are not Associated with Poor Outcome in Patients with ICH Following a Stereotactic Minimally Invasive Surgery
Authors: Yang Xu , Zhang Linshan , Wang Likun , Mao Yuanhong , Li Yinghui , Luo Jingbiao , Wu Guofeng 
Published: July 2020

Abstract
Backgrounds: The initial CT blend sign has been used as an imaging maker to predict haematoma expansion and poor outcome in patients with small volume intracerebral haemorrhage (ICH). However, the relationship between the blend sign and the outcome of patients remains elusive. The present study aimed to retrospectively observe the impact of initial CT blend signs on the short-term outcome of patients with hypertensive ICH underwent a stereotatic minimally invasive surgery (sMIS). Methods: Two hundreds and forty-two patients with spontaneous ICH were enrolled. Based on the initial CT features, the patients were assigned to a blend sign group (including 91 patients) or a non-blend sign group (control group, including 151 patients). The NIHSS, GCS and the mRS were used to observe the efficacy of the sMIS. The rates of severe pulmonary infection, cardiac complications were also compared between the two groups. Results: No significant differences in NIHSS and GCS in one week or two weeks after surgery were observed between the two groups. The proportion of patients with good outcome during follow-up did not show any difference between the two groups. However, both the two groups displayed good functional outcome over admission. The rate of rehaemorrhage increased in the blend sign group. No significant differences in severe pulmonary infections and cardiac complications were noted between the two groups. Conclusions：The initial CT blend sign are not associated with poor outcome of patients with hypertensive ICH underwent sMIS. ICH patients with CT blend sign should be also treated by performing sMIS if they are candidates for surgery.