bluuebunny/crossref_metadata_2025_split huggingface dataset:
Construction of a sensitive and specific lead biosensor using a genetically engineered bacterial system with a luciferase gene reporter
Authors: Nourmohammadi Esmail , Hosseinkhani Saman , Nedaeinia Reza , Khoshdel-Sarkarizi Hoda , Nedaeinia Mozhdeh , Ranjbar Maryam , Ebrahimi Neshat , Farjami Zahra , Nourmohammadi Mohammad , Mahmoudi Ali , Goli Mohammad , A.Ferns Gordon , Sadeghizadeh Majid 
Published: March 2020

Abstract
Background: A bacterial biosensor refers to genetically engineered bacteria that produce an assessable signal in the presence of a physical or chemical agent in the environment. Methods: We have designed and evaluated a bacterial biosensor expressing a luciferase-reporter gene controlled by pbr and cadA promoters in Cupriavidus metallidurans (previously termed Ralstonia metallidurans) containing CH34 and pI258 plasmids of Staphylococcus aureus, respectively, and that can be used for the detection of heavy metals. In the present study, we produced biosensor plasmids designated pGL3-luc/pbr-biosensor and pGL3-luc/cad-biosensor, that were based on the expression of luc+ under the control of the cad promoter and the cadC gene of S. aureus plasmid pI258 and pbr promoter and pbrR gene from plasmid pMOL30 of Cupriavidus metallidurans.Results: We found that the biodegradable pGL3-luc/pbr-biosensor could be used to measure lead concentrations between 1-100 μM in the presence of other metals, including: zinc, cadmium, tin and nickel. The latter metals did not result in gene expression of the reporter. The pGL3-luc/cad-biosensor was able to detect lead concentrations between 10 nM to 10 μM.Conclusions: This biosensor was found to be a specific for measuring lead ions in both environmental and biological samples.
Stem cell membrane-coated isotretinoin for acne treatment
Authors: Wang Shiyi , Jiang Rihua , Meng Tianqi , Zhang Fuqiang , Li Jing , Jin Yongri , Lee JeungHoon , Zhu Mingji , Jiang Jinlan 
Published: March 2020

Abstract
Background: Topical isotretinoin is commonly used to treat acne. However, topical isotretinoin has side effects and can permeate through the stratum corneum, the most important skin barrier. Therefore, this study aimed to demonstrate the efficacy of nanoparticles as stable carriers with great curative effects, low side effects, and strong transdermal ability.Results: In a rabbit model of hyperkeratinization, STCM-ATRA-NPs showed significant therapeutic efficacy. By contrast, negative therapeutic efficacy was observed in a golden hamster model of hyper sebum production. Scanning electron microscopy and Fourier transform infrared spectral analyses showed that nanoparticles could penetrate the stratum corneum. Western blotting demonstrated that the nanoparticles could enhance the transdermal efficacy of isotretinoin by reducing the effect of keratin and tight junction proteins. Further, nanoparticles enhanced endocytosis, thereby promoting drug penetration and absorption into the skin.Conclusion: STCM-ATRA-NPs were demonstrated to control isotretinoin release, reducing its side effects and efficiently permeating through the skin by reducing the effect of keratin and tight junction proteins and enhancing endocytosis.
Comparison of Clinical Outcomes Between Total Knee Arthroplasties Using Medial-Pivot and Posterior-Stabilized Prosthesis: Minimum 8-year Follow-up Results in Chinese population
Authors: Gao Zheng-yu , Guo Cui-cui , Zhang Zi-an , Shang Guang-qian , Yan Wei-ning , Xiang Shuai , Zhang Hai-ning , Wang Ying-zhen , Xu Hao 
Published: March 2020

Abstract
Objective To compare the clinical outcomes of Medial-pivot and Posterior-stablized total knee arthroplasty during 8 years follow-up. Methods 109 patients with osteoarthritis who underwent primary total knee arthroplasty were conducted and divided into two groups according to the choice of prosthesis,49 in the Medial-pivot(MP) group and 60 in the Postirior-stabilized(PS) group.There were no statistical differences in age,gender,BMI,operative side and operation time between the two groups (P>0.05).The same protocol of perioperative treatment was adopted in both groups. Comparison of clinical outcome and survival rates were acquired through minimum 8 years follow-up. Results The prosthesis survival rate andthe excellent and good rate of MP group were 93.9% and 88.9% during average 8.3 years follow-up period,versus the prosthesis survival rate and the excellent or good rate of PS group were 93.3% and 86.7% during average 8.4 years follow-up period.There were no statistical diferences in survival rate,excellent and good rate and radiolucency of weight-bearing areas between the two groups ( P >0.05). There were no statistical differences in range of motion,KSS score and WOMAC index between the two groups preoperatively (P>0.05).The range of motion at all follow-up time points and KSS score at 6 months postoperatively of PS group were superior than those of MP group ( P <0.05),while the WOMAC index at 8 years postoperatively and Kujala scores at 1year and 8 years postoperatively of PS group were better than those of MP group( P <0.05). Conclusion Either medial-pivot or post-stabilized total knee arthroplasty could obtain excellent or good mid-term clinical result. Although with less range of motion, the patients using MP prosthesis could obtain better results in bone loss, performance of the patellofemoral joint and mid-term satisfaction. However, the use of MP prosthesis required more professional and precise technology and it should be cautious when using for knees with severe valgus deformation.
Comparison of Calculated Skin Doses in Cineradiography and Four Dimensional Kinematic Computed Tomography of the Wrist
Authors: Goelz Leonie , Asmus Ariane , Kim Simon , Diehl Joscha , Eisenschenk Andreas , Schüler Gabriele , Mutze Sven 
Published: March 2020

Abstract
Background: Previous studies have discussed the diagnostic value of four dimensional kinematic CT in cases of carpal instabilities. This analysis compares calculated skin doses of 4D CT and conventional cineradiography of the wrist in cases of suspected SLL rupture.Methods: Retrospective calculation and interpolation of skin doses and effective doses for ten consecutive 4D CT examinations and 41 cineradiographies for suspected lesions of the scapholunate ligament. Standardised anterior-posterior and lateral cine sequences using a flat-panel digital subtraction imager and of 4D kinematic CT using a dual-source scanner were acquired and acquisition parameters recorded. We tested if the skin dose of 4D CT is different from cineradiography. Results: Median dose area product (DAP) of cineradiography was 135.34 cGycm 2 resulting in a calculated median skin dose of 32.6 mSv (confidence interval 26.86-42.90 mSv) and an estimated effective skin dose of 3.26 µSv. CT dose index (CTDI) for 4D examinations was recorded to be 26.79 mGy and the dose-length product (DLP) was 150 mGy*cm. This resulted in an estimated skin dose of 34 mSv, which is covered by the confidence interval of cineradiography, and an effective skin dose of 3.4 µSv. Conclusions: Skin dose calculations are comparable for 2D cineradiography in two plains and 4D kinematic CT of the wrist. Calculated effective doses are <0.01 mSv.
Diagnosis of left ventricular hypertrophybased on convolutional neural network
Authors: Jian Zini , Wang Xianpei , Zhang Jingzhe , Wang Xinyu , Deng Youbin 
Published: March 2020

Abstract
Background: Clinically, doctors obtain the left ventricular posterior wall thickness (LVPWT) mainly by observing ultrasonic echocardiographic video stream to capture a single frame of images with diagnostic significance, and then mark two key points on both sides of the posterior wall of the left ventricle with their own experience for computer measurement. In the actual measurement, the doctor's selection point is subjective, which is not only time-consuming and laborious, but also difficult to accurately locate the edge, which will bring errors to the measurement results.Material/Methods: In this paper, a convolutional neural network model of left ventricular posterior wall positioning was built under the TensorFlow framework, and the target region images were obtained after the positioning results were processed by non-local mean filtering and opening operation. Then the edge detection algorithm based on threshold segmentation is used. After the contour was extracted by adjusting the segmentation threshold through prior analysis and the OTSU algorithm, the design algorithm completed the computer selection point measurement of the thickness of the posterior wall of the left ventricle. Results: The proposed method can effectively extract the left ventricular posterior wall contour and measure its thickness. The experimental results show that the relative error between the measurement result and the hospital measurement value is less than 15%, which is less than 20% of the acceptable repeatability error in clinical practice. Conclusions: Therefore, the method proposed in this paper not only has the advantage of less manual intervention, but also can reduce the workload of doctors.
Development Of An Excel-Based Cost Effectiveness Analysis Tool For Ascertaining Value For Money In Implementation Of Hiv And Other Health Interventions At Bomu Hospital, Kenya
Authors: NJERU NANCY MUCOGO , Owiti Elizabeth , Ahmed Aabid 
Published: March 2020

Abstract
An Organization Capacity Assessment done in Bomu hospital in April 2015 with an aim to strengthen the local institution’s capacity for sustainable fight against HIV Aids pandemic led to the development of this excel-based cost effectiveness analysis tool. Objective: The specific objectives were to develop a cost-effectiveness analysis excel tool complete with instruction manual for use in costing, determining prices of services and carrying out economic evaluations. Method: A technical working team (TWG) comprising of senior management of the hospital and the University of Nairobi Fellow was formed. The TWG identified program where cost indicators would be derived, developed cost indicators collection tools, collected costing data and build necessary consensus and assumptions. The data was used to design the tool complete with working instructions. Results: The tool comprises of processes, instructions, excel data entry provisions and inbuilt formulae in excel sheets. The unique tool features include; simple to use instructions, systematic listing of cost elements with a drop down option to allow selection as required, allows additional cost elements to be added thus increasing scope of use . The tool also separates costs and sub-costs in a manner that allows cost drivers to be known precisely as well as avoid double costing among others. Conclusion: The tool is transferable to other facilities and can be replicated in all hospitals within the country, in private, public and non – governmental organizations. Its regular review, improvements and utilization will be important for the health sector to fully benefit from its use. It will be necessary to sensitize health facilities to address information gaps and also ensure that data is available in form and detail necessary for costing purposes. Key words: Economic evaluation, Costing, Resources, Treatment outcomes
The State and Biochemical Composition of Coniferous Plant Wood in Places of Degradation
Authors: Bukharina Irina , Vedernikov Konstantin , Pashkova Anna , Zagrebin Yegor 
Published: March 2020

Abstract
Background. A significant deterioration in the state of dark coniferous forests, which in some cases is accompanied by their drying, has manifested itself throughout the Northern Hemisphere. This process is very dynamic and covers the entire boreal zone from Europe to the North American continent, covering all forest-forming species Research in the field of the ecological biochemistry of wood opens up wide possibilities for studying the stability of spruce stands. Coniferous wood, including spruce, consists of polymer structural components (cellulose, hemicellulose and lignin) and non-structural components (including extractives, ash and other minor substances). Extractives with high biological activity, which are produced as protective compounds during the life of plants from external environmental stresses, play an important protective function in coniferous wood. The structure of wood and its biochemical composition can change under various influences. The content of extractives varies greatly not only in different areas, but also from specimen to specimen, depending on their condition. In this regard, the aim of our research was to study the content of extractives in spruce wood in specimens of different life states.Methods. This work presents the results of studies on the characteristics of the biochemical composition of Siberian spruce (Pícea obovata L.) in mass drying conditions in the territory of the Udmurt Republic (Russia). The enumeration method was used to create a plot taxation. A study of the biochemical composition of wood was carried out based on the content of extractives from specimens in various life states. Results. Trial plot stands are characterized by low tree density in the main canopy. According to biochemical analysis, the highest content of extractives was observed in specimens with good and satisfactory vitality. In trees with an unsatisfactory life state, biochemical parameters were low, which is associated with the lack of self-regulation in dead wood. Conclusions. Obviously, the substances under study play a large role in the adaptive reactions of Pícea obovata, and the increase in their production is a response to negative environmental factors.
Diagnostic value of the lymphocyte-to-monocyte and albumin-to-globulin ratios in patients with colon cancer
Authors: Huang Li , Mo Zhuning , Hu Zuojian , Zhang Linyan , Qin Shanzi , Qin Xue , Li Shan 
Published: March 2020

Abstract
Background: Ample evidence has revealed that the lymphocyte-to-monocyte ratio (LMR) and albumin-to-globulin ratio (AGR) are cancer-related inflammatory markers. The present study aimed to assess whether LMR and AGR, alone or in combination with carcinoembryonic antigen (CEA), was a useful diagnostic marker for colon cancer.Methods: This retrospective study enrolled 251 patients with colon cancer, 171 patients with benign colon diseases, and 187 healthy control subjects from January 2012 to September 2019. Mann-Whitney U test or Chi-square test were used to analyze differences between groups in laboratory parameters and clinicopathological features. The diagnostic value of LMR and AGR combined or not with CEA in colon cancer patients was determined via a receiver operating characteristic (ROC) curve.Results: The levels of LMR and AGR were decreased in the colon cancer group compared with the healthy control and benign colon disease groups. The LMR and AGR were correlated with lymph node metastasis, tumor size, and clinical stage. Moreover, AGR was associated with distant metastasis. Both the LMR (r = −0.137, p = 0.030) and AGR (r = −0.178, p = 0.005) were negatively correlated with the concentration of CEA. LMR or AGR combined with CEA could enhance sensitivity (75.30% for LMR + CEA, 58.57% for AGR + CEA) and generate larger area under curve (AUC; 0.75 for LMR + CEA, 0.74 for AGR + CEA) compared with the LMR (p < 0.001), AGR (p < 0.001), or CEA (p < 0.001) alone.Conclusion: The combination of LMR or AGR with CEA may enhance the sensitivity and diagnostic efficacy of detecting colon cancer from benign colon diseases.
Knowledge and practice of postoperative nausea, vomiting management, and predictors of knowledge among health professionals in referral hospitals of northwest Ethiopia, 2019
Authors: Yetneberk Tikuneh , Fentie Yewlsew , Yimer Hailu , Belayneh Tadesse , Tarekegn Abraham 
Published: March 2020

Abstract
Background Good knowledge conjoined with evident practice of postoperative nausea, and vomiting management is vital for the optimal management of patients. On the other hand, inadequate knowledge and poor practice of health professionals on postoperative nausea vomiting risk reduction and management come up with complications, reduce patient satisfaction, increase patient, and hospital costs. Objective To assess knowledge, practice, and predictors of knowledge regarding postoperative nausea and vomiting management among health professionals working in referral hospitals in northwest Ethiopia. Methods A multicenter cross-sectional study was conducted on 407 health professionals from February 27 to March 30, 2019, in referral hospitals in northwest Ethiopia. Stratified random Sampling followed by simple random sampling was used to select the study participants. Both bivariable and multivariable logistic regression were used to identify factors associated with the knowledge level of health professionals in PONV management. In the multivariable analysis, variables with a p-value <0.05 were considered statistically significant. Results 52.8% of the study participants had good knowledge of PONV management (95% CI: 47.9-57.2). Male professionals were 1.95 times more likely knowledgeable as compared with females. The odds of physicians being knowledgeable were 5.36 times more likely than midwives, while the odds of anesthetists being knowledgeable were 3.88 times more likely than midwives. Professionals trained on PONV management were 5.32 (AOR: 5.32; 95%CI: 1.58, 17.89) times more likely knowledgeable than those who did not train. Conclusions Nearly fifty % of healthcare providers working in the perioperative working areas had poor knowledge of PONV management. Gender, profession, and training on PONV management were factors significantly affecting the knowledge level of health professionals. Regular training seminars, continuous professional development, adherence to the latest guidelines, and conducting clinical audits are recommended to increase knowledge and improve the practices of health professionals in postoperative nausea vomiting management.
Exploring the Pharmacological Mechanism of the modified Chinese medicine formulas “Shenfu-Linguizhugan decoction” treating Dilated cardiomyopathy Based on Network Pharmacology
Authors: Quan Wuxia , Miao Yandong 
Published: March 2020

Abstract
Background: Dilated cardiomyopathy (DCM) is a non-ischaemic cardiac muscle disease with structural and functional myocardial aberration can lead to extensive morbidity and mortality due to complications in particular heart failure and arrhythmia. Two classic Chinese medicine formulas, Shenfu decoction and Linguizhugan decoction, were both shown to exert therapeutic effects on heart disease. Thus, modified Shenfu and Linguizhugan decoction (SFLGZGD) is recommended for treatment DCM. However, its chemical and pharmacological characteristics remain to be elucidated. In the current study, a network pharmacology approach was applied to characterize the action mechanism and target genes of SFLGZGD on DCM.Methods: The gene expression of DCM was obtained from the Gene Expression Omnibus (GEO). All compounds were obtained from the correlative databases, and active mixture were selected according to their oral bioavailability (OB) and drug-likeness (DL) index. The potential targets of SFLGZGD were obtained from the traditional Chinese medicine systems pharmacology (TCMSP) database. The compound-target and target-pathway networks were constructed. The protein-protein interactive (PPI) network generated by R software was visualized by Cytoscape, and the topology scores, functional regions, and gene annotations were analyzed using plugins of Bisogenet and CytoNCA. The potential pathways related to target genes were determined by gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses.Results: A total of 963 differentially expressed genes (DEGs), including 538 upregulated genes and 425 downregulated, were obtained from GSE19303. A total of 636 ingredients in SFLGZGD were obtained, among which, 93 were chosen as bioactive components. The compound-target network included 10 bioactive components and 18 potential targets and a total of 1939 genes obtained in the PPI network, among them, a total of 16 genes were screened out. Moreover,129 terms on the GO analysis and six pathways obtained. Among these potential targets, EGFR, CDKN1A, MMP1, COL1A1, COL3A1, MMP3, ICAM1, and HSPB1 were identified as relatively high-degree targets.Conclusions: The network pharmacology-based approach in the current study has shown promising potential in identifying major therapeutic targets from TCM formulations. Besides, our study suggested that network pharmacology prediction may provide a useful tool for describing the molecular mechanism of SFLGZGD on DCM.
Isolation of a Pseudomonas putida strain that degrades p-hydroxybenzoic acid from the soil of a Panax ginseng field
Authors: Shen Liang , Zhu Guangwei , Guo Shuai , Li Xiwen , Xiao Shuiming , Xu Jiang , Chen Shilin 
Published: March 2020

Abstract
Background: Panax ginseng is a valuable herbal medicine which has been applied in eastern Asia to maintain their physical vitality for millennia. However, continuous cropping leads to the accumulation of allelochemicals, which subsequently reduces the quality and yield of P. ginseng, and arable soils for P. ginseng cultivation are getting scarce. Methods: In this study, a strain highly efficient degrading p-hydroxybenzoic acid was isolated from the rhizosphere soil of a ginseng cultivation field. Subsequently, its draft genome was sequenced, and the key genes involving the p-hydroxybenzoic acid-degrading pathway were identified by bioinformatics software. Results: Based on its biophysical/biochemical characteristics, analysis of the 16S rRNA sequence and gyrB gene authentication, strain LD6 was identified as Pseudomonas putida LD6. The initial p-hydroxybenzoic acid concentration in minimal salt medium was 100 mg•L-1, and after 8 d of bacterial culture in the laboratory, the degraded reached 97.35%. The p-hydroxybenzoic acid content was decreased by 35.21%, and the ginseng death rate was decreased by 38.46% in the rhizosphere soil of P. ginseng inoculated with strain LD6. The draft genome sequence consisted of 5,765,634 bp, and the genome comprised 5186 protein-coding genes, and the pobA, catB, pcaG, UbiA, and UbiX genes were annotated, and may play important roles in p-hydroxybenzoic acid degradation.Conclusion: Isolation and manipulation of functional bacteria is an effective strategy to alleviate ginseng continuous cropping problems and enhance the quality and yield of P. ginseng.
Assessment of the explosive chamber in the projector system of Hayabusa2 for asteroid sampling
Authors: Takano Yoshinori , Yamada Keita , (Deceased) Chisato Okamoto , Sawada Hirotaka , Okazaki Ryuji , Sakamoto Kanako , Kebukawa Yoko , Kiryu Kento , Shibuya Takazo , Igisu Motoko , Yano Hajime , Tachibana Shogo 
Published: March 2020

Abstract
We report an assessment of the explosive chamber in the projector system used during the sampling operation of the Hayabusa2 project at the surface of the C-type asteroid Ryugu. Although the explosion process was designed as a closed system (cf. Sawada et al., Space Sci. Rev., 2017), volatile combustion gases and semivolatile organics were produced together with quenched carbonaceous products. The chemical compositions of the gases, organics, and inorganics were investigated in the screening analysis. A solid-phase microextraction technique and thermal desorption coupled with gas chromatography/mass spectrometry analysis revealed that aliphatic ( three rings > four rings, resulting in abiogenic molecular patterns. The most intense inorganic fingerprints were due to potassium (K + ) and chloride (Cl – ) ions derived from the initial KTB explosive and RK ignition charge. We discuss quality control and quality assurance issues applicable to future sample processes during the Hayabusa2 project.