bluuebunny/crossref_metadata_2025_split huggingface dataset:
Severe Pantothenic Acid Deficiency Induces Alterations in the Intestinal Mucosal Proteome of Starter Pekin Ducks
Authors: Tang Jing , Feng Yulong , Zhang Bo , Wu Yongbao , Guo Zhanbao , Liang Suyun , Xie Ming , Zhou Zhengkui , Hou Shuisheng 
Published: June 2020

Abstract
Background: Pantothenic acid deficiency (PAD) results in growth depression and intestinal hypofunction of animals. The underlying mechanisms, however, remain to be established and an overview of molecular alterationsis still lacking.We investigated intestinal mucosalproteome changes induced by PADin ducks to explain its effects on growth and intestine.Methods:A total of128 one-day-oldPekinducks were divided into two groups,with 8 replicates and 8 birds per replicate. All the ducks were fed either a PAD or a pantothenic acid adequate (control, CON) dietfor 16 days.Results:High mortality, growthretardation,fasting hypoglycemia,reduced plasma insulin, and oxidative stresswere observed in the PAD group compared to the CON group. Furthermore, PAD induced morphological alterationsof the small intestine indicated by reduced villus height and villus surface area of duodenum, jejunum, and ileum.The duodenum mucosal proteome of ducks showed that 198 proteins were upregulated and 223 proteins were downregulated (> 1.5-fold change) in the PAD group compared to those in the CON group. Pathway analysis of these proteins exhibited the suppression of glycolysis and gluconeogenesis, fatty acid beta oxidation, tricarboxylic acid cycle, oxidative phosphorylation, oxidative stress, and intestinal absorption in the PAD group, indicating impaired energy generation and abnormal intestinal absorption.Wealso show that nine out of eleven proteins involved in regulation of actin cytoskeleton were upregulated by PAD, probably indicates reduced intestinal integrity.Conclusion:PAD leads to growth depression and intestinal hypofunctionof ducks, which are associated with impaired in energy generation, abnormal intestinal absorption, and regulation of actin cytoskeletonprocesses. These findings contribute to our understanding of the mechanisms of intestinal mucosa metabolic disorders due to PAD.
Therapeutic and Preventive Effects of a New Type Healthy and Viable Food Supplement on Fatty Liver and Blood Lipids in Animal Model
Authors: Bayat Azam , Khalkhali Aref , Mahjoub Ali Reza 
Published: June 2020

Abstract
Background: Fatty liver disease is one of the most common liver complications worldwide. Also, blood lipids are elevated cholesterol or triglyceride levels from the normal amount in the blood that causes fatty liver disease. The aim of this study was to investigate the therapeutic effects of a new healthy and viable food supplement from wheat on fatty liver disease and blood lipids in animal model.Methods: The new NBS healthy and viable food supplement was prepared by a green route. The NBS healthy and live food powder had various vitamins, macro and micro molecules, and ingredients such as B1, B2, B3, B5, B6, B9, C, K, A, E, D, phosphorus, potassium, sulfur, magnesium, calcium, iron, manganese, zinc, copper, omega-3 and etc. For therapeutic and preventive effects of the new healthy and viable dietary supplement on fatty liver and blood lipids, 25 Wistar rats weighing 180–220 g were used. The rats were divided into 5 groups and were treated with 25, 50 and 100 mg/kg of healthy and live medication.Results: Investigation of the interaction between the concentrations of the medication supplement showed that 100 mg/ kg has the most therapeutic effect against oasises in fatty liver disease. Also, it was found that the concentration of 1000 mg/ kg has the most reducing effect on the level of lipid profile.Conclusions: The new food supplement reduces the level of hepatic macrovesicles, microvesicles, and the steatosis symptoms without specific hepatic complications. Also, the healthy food causes reduction of lipid parameters.
Retroperitoneal Hemorrhage During Veno-Venous Extracorporeal Membrane Oxygenation in COVID-19 Patientss: Clinical Experience and Review of Literature
Authors: Zhang Jingchen , He Xujian , Hu Jia , Li Tong 
Published: June 2020

Abstract
Background: Retroperitoneal hemorrhage is a rare and severe complication in patients undergoing extracorporeal membrane oxygenation (ECMO). Retroperitoneal hematoma after the operation of multiple ECMO in a single center at the same time is extremely rare. The causes and treatment options can provide clinical experience and bring some inspiration.Case presentation: Three cases of retroperitoneal hemorrhage patients with Corona Virus Disease-19 (COVID-19) are introduced; they had respiratory failure and were treated with veno-venous ECMO or veno-arterial-venous ECMO. Retroperitoneal hemorrhage occurred during ECMO treatment. Among the three cases, 2 cases were found due to abdominal pain, and 1 case was found because of a decrease in ECMO circuit flow rate and hemoglobin level. 2 cases were treated with transcatheter arterial embolization, and 1 case was treated conservatively. The hemorrhage in each of the 3 cases did not deteriorate. Through early diagnosis and treatment, satisfactory treatment results were achieved for these 3 patients.Conclusions: During the period of ECMO treatment, there is a low incidence of retroperitoneal hematoma, yet it comes with a high risk. This is due to anticoagulant use and some local mechanical injuries. If a decline in blood flow velocity and hemoglobin is detected, retroperitoneal hematoma should be taken into consideration, and early aggressive therapy should be started.
Genipin Suppresses Growth and Metastasis in Hepatocellular Carcinoma Through Blocking Activation of STAT-3
Authors: Hong Ming , Lee Selena , Clayton Jacob , Yake Wildman , Li Jinke 
Published: June 2020

Abstract
Background Signal transducer and activator of transcription-3 (STAT-3) can facilitate cancer progression and metastasis by being constitutively active via various signalling. Abundant evidence has indicated that STAT-3 may be a promising molecular target for cancer treatment. Methods In this study, a dual-luciferase assay-based screening of 537 compounds for STAT-3 inhibitors of hepatocellular carcinoma (HCC) cells was conducted, leading to the identification of genipin. Effects of genipin on HCC were assessed in patient-derived xenograft mice model. Western blotting assay, chromatin immunoprecipitation (ChIP) assay, molecular docking study, tube formation assay, three-dimensional top culture assay, histological examination and immunofluorescence were utilized to evaluate the regulatory signalling pathway. Results Our research have demonstrated that genipin suppresses STAT-3 phosphorylation and nuclear translocation, which may attribute to the binding capacity of this compound to the Src homology-2 (SH2) domain of STAT-3. In addition, we also demonstrated the therapeutic effects of genipin in patient-derived HCC xenograft mice model.Conclusion In conclusion, genipin showed therapeutic potential for HCC treatment by interacting with SH2-STAT-3 domain and suppressing the activity of STAT-3. In future study, further research are expected for exploring the potential role of genipin in combination with chemotherapy or radiotherapy for HCC.
3D-Printed Screw-Rod Auxiliary System for Unstable Atlas Fractures: A Retrospective Analysis
Authors: Wu Chao , Deng Jiayan , Li Tao , Pan Jian , Hu Haigang , Tan Lun 
Published: June 2020

Abstract
Objective To develop and validate a 3D-printed screw-rod auxiliary system for unstable atlas fractures.Methods Fourteen patients who underwent occipitocervical fusion with the 3D-printed screw-rod auxiliary system enrolled in our hospital from 2017 to 2019 were reviewed. The operation time, blood loss and radiation times during the operation were recorded. The maximum fracture displacement values pre- and post-operation were measured based on CT imaging. All screw grades were evaluated after surgery. The O-C2 angle and OCI angle pre-operation, post-operation and at the last follow-up were measured. The dysphagia scale 3 and 12 months after surgery and the NDI 3 and 12 months after surgery were assessed.Results The average surgery time, average blood loss and average radiation times for the 14 patients were 112.14 minutes, 171.43 ml and 5.07 times, respectively. There was a significant difference in maximum fracture displacement between pre- and post-operation values (P<0.05). A total of 56 screws were inserted; 3 screws were classified as grade 1, and the others were classified as grade 0. There was a significant difference in the O-C2 and OCI angles from pre-operation values to values 3 days after the operation (P=0.002, P<0.05); there was no significant difference in the O-C2 or OCI angle from 3 days after the operation to the last follow-up (P=0.079; P=0.201). The dysphagia scales of two patients were assessed as mild 3 months after surgery, and the others were assessed as normal. All patients’ dysphagia scores returned to normal 12 months after surgery. The average NDI and average neck VAS scores 12 months after surgery were 2.53 and 8.41, respectively.Conclusion It is clinically feasible with the assistance of a screw-rod auxiliary system to perform occipitocervical fusion for unstable atlas fractures. This novel technique can objectively restore the occipitocervical angle of patients, and there are few postoperative complications.
Predictive hazard markers for the ranking of pulmonary toxicity of nanomaterials
Authors: Nishida Chinatsu , Izumi Hiroto , Tomonaga Taisuke , Takeshita Jun-ichi , Yamasaki Kei , Yatera Kazuhiro , Morimoto Yasuo 
Published: June 2020

Abstract
Background

We analyzed the mRNA expression of chemokines in rat lungs following intratracheal instillation of nanomaterials in order to find useful predictive markers of the pulmonary toxicity of nanomaterials. Four nanomaterials, nickel oxide (NiO) and cerium dioxide (CeO2) as nanomaterials with high pulmonary toxicity, and titanium dioxide (TiO2) and zinc oxide (ZnO) as nanomaterials with low pulmonary toxicity, were intratracheally administered into rat lungs at 0.8 mg/kg BW or 4 mg/kg BW as low and high doses, respectively. Five chemokine genes (C-X-C motif chemokine 5 (CXCL5), C-C motif chemokine 2 (CCL2), C-C motif chemokine 7 (CCL7), C-X-C motif chemokine 10 (CXCL10), and C-X-C motif chemokine 11 (CXCL11) ) were selected using cDNA microarray analysis at 1 month after instillation of NiO in the high dose group. The mRNA expression of these 5 genes in the lung tissues were evaluated at 3 days, 1week, 1 month, 3 months and 6 months after instillation of the 4 nanomaterials, using real-time quantitative polymerase chain reaction (RT-qPCR) during the observation periods.
Results

An increased expression of CXCL5 in the NiO and CeO2 groups was persistently higher than in the control group, and that in the TiO2 and ZnO groups was transiently higher than in the control group. CCL2 and CCL7 also showed an increasing tendency similar to CXCL5, although there was not a persistent significance. The receiver operating characteristic (ROC) results of nanomaterial toxicity with the expression level of each gene showed a considerable relationship between the pulmonary toxicity ranking of nanomaterials and the expression of CXCL5, CCL2 and CCL7, with area under the curves (AUC) of 0.95 or higher at 1 week and 1 month. The expression levels of these 3 genes also moderately or strongly correlated with the degree of inflammatory cells in the lung tissues.
Conclusion

Our results suggest that 3 chemokine genes, especially CXCL5, followed by CCL2 and CCL7, can be useful as biomarkers for the ranking of the pulmonary toxicity of nanomaterials.
The diets of sows with fish oil might decrease the oxidative stress and inflammatory response in sows, but increase the susceptibility to inflammatory response in their offspring
Authors: Luo Wenli , Zhang Jing , Xu Weina , Xu Jianxiong 
Published: June 2020

Abstract
Backgroup: This aim of this study was to investigate that the effect of supplement maternal diet with fish oil on the oxidative stress and inflammatory response of sows and their offspring.Methods: Twelve sows were divided into two groups. Sows were fed soybean oil diet (SD) or soybean oil + fish oil diet (FD) from gestating to lactating period. The blood samples of lactating sows were collected. At the age of 14 days, one piglet was selected from each litter. After the blood was collected from the anterior vena cava, LPS was injected into the neck muscle. The blood was collected 5 hours after LPS injection. At 48h after LPS injection, blood and liver samples were collected.Results: Maternal fish oil supplementation improved the health of sows by increasing the level of HDL-C and decreasing the levels of AKP and TNF-α in the plasma of sow (P<0.05), and induced a positive effect on litter immune status associated with a modification of both IgG in the colostrum and IL-10 in the milk(P<0.05).In addition, antioxidant capacity in piglets increased by improving the level of GSH-Px and T-AOC (P<0.05) in the plasma of piglets 48h after LPS challenged. Meanwhile, the expression of GSH-Px mRNA and p-ERK protein in the livers of piglets was inhibited (P<0.05). However, in FD group, the level of IL-1β and IL-6 in the plasma of piglets were significantly higher before and after LPS challenged (P<0.05). The expression of NF-κB mRNA and p-IκB-α protein was increased in the liver of piglets (P<0.05). Conclusion: These results indicated that the diet of sow with fish oil might decrease the oxidative stress and inflammatory response in sows and enhance the antioxidative ability in their progenies, but might increase the susceptibility to inflammatory response in progenies.
Possible associations between callers’ degree-of-worry and their socioeconomic status when contacting out-of-hours services: a prospective cohort study
Authors: Gamst-Jensen Hejdi , Thilsted Sita LeBlanc , Folke Fredrik , Tolstrup Janne S. , Thygesen Lau Caspar 
Published: June 2020

Abstract
Background:

Telephone triage within out-of-hours (OOH) services aims to ascertain the urgency of a caller’s medical condition in order to determine the correct type of health care needed, ensuring patient safety. To improve the triage process by increasing patient-centred communication, a triage tool has been developed, whereby callers are asked to rate their degree-of-worry (DOW) as a measure of self-evaluated urgency. Studies show that low socioeconomic status (SES), being single and non-Western ethnicity are associated to low self-rated health and morbidity and these factors may also be associated with high DOW. The aim of this paper was to examine if low SES, being single and non-Western ethnicity were associated to high DOW of callers contacting OOH services.
Methods:

A prospective cohort study design, at the OOH services for the Capital Region of Denmark. Over two weeks, 6,869 of 38,787 callers met the inclusion criteria: ≥18 years, patients themselves or close relative/friend, reported DOW, had a valid personal identification number and gave informed consent. Callers were asked to report their DOW (1 = minimal worry to 5 = maximal worry), which was dichotomized into low (1–3) and high (4–5) DOW and linked to data from electronical medical records and Statistics Denmark. Socioeconomic factors (education and annual household income), marital status and ethnicity were assessed in relation to DOW by logistic regression.
Results:

High DOW was reported by 38.2% of the participants. Low SES (low educational level; OR 1.5, 95% CI 1.3–1.7 and low annual household income; 1.5, 1.3–1.6) was associated with high DOW and so too was being single; 1.2, 1.1–1.3 and of non-Western ethnicity; 2.9, 2.5–3.4.
Conclusions:

Knowledge of the association of low SES, marital status as single and non-Western ethnicity with high DOW among callers to OOH services may give call handlers a better understanding of callers’ DOW. If this does not correspond to the call handler’s perception of urgency, this knowledge may further encourage patient-centred communication, aid the triage process and increase patient safety. A better understanding of socioeconomic variables and their relation to callers’ DOW gives direction for future research to improve telephone triage of OOH services.
Low-dose warfarin combined with low-dose aspirin associated Spinal intramedullary hemorrhage: a case report
Authors: Zhou Pan , Liu Ren-li , Zhang Ke-bao , Liu De-hong , Deng Zhe 
Published: June 2020

Abstract
Background:Spinal intramedullary hemorrhage is a rare event, the disease still has not received enough attention and many characteristics of the disease have not been sufficiently explored. Due to its rarity, it can easily be forgotten or misdiagnosed. Case presentation:We report a case of 72-year-old male patient with a medical history of coronary heart disease, atrial fibrillation (AF), hypertension, cervical spondylosis, carotid and vertebral artery stenosis with low-dose anticoagulation combined with low-dose antiplatelet therapy who suffered from sudden posterior neck and back pain for 3 hours visited our emergency department (ED). The International Normalization Ratio (INR) were controlled well, and other laboratory tests were within normal limits or only slightly abnormal. However, the patient was diagnosed with spinal intramedullary hemorrhage and died finally. Conclusion:Anticoagulation combined with antiplatelet-associated rare and life-threatening complications should be paid more attention.
Discontinuation of Hydroxychloroquine in Older Patients with Systemic Lupus Erythematosus: A Multicenter Retrospective Study
Authors: Ruiz Ruth Fernandez , Bornkamp Nicole , Kim Mimi Y. , Askanase Anca D. , Zezon Anna , Tseng Chung-E , Belmont H. Michael , Saxena Amit , Salmon Jane E. , Lockshin Michael , Buyon Jill P. , Izmirly Peter M. 
Published: June 2020

Abstract
Background: Although hydroxychloroquine (HCQ) is a mainstay of treatment for patients with Systemic Lupus Erythematosus (SLE), ocular toxicity can result from accumulated exposure. As the longevity of patients with SLE improves, data are needed to balance the risk of ocular toxicity and the risk of disease flare, especially in older patients with quiescent disease. Accordingly, this study was initiated to examine the safety of HCQ withdrawal in older SLE patients.Methods: Data were obtained by retrospective chart review at three major lupus centers in New York City. Twenty-six patients who discontinued HCQ, and thirty-two patients matched for gender, race/ethnicity and age were included in this study. The primary outcome was the occurrence of a lupus flare classified by the revised version of the Safety of Estrogens in Lupus Erythematosus: National Assessment version of the Systemic Lupus Erythematosus Disease Activity Index (SELENA-SLEDAI) Flare composite index, within one year of HCQ withdrawal or matched time of continuation.Results: Five patients (19.2%) in the HCQ withdrawal group compared to five (15.6%) in the HCQ continuation group experienced a flare of any severity (odds ratio [OR] = 1.28; 95% CI: 0.31, 5.30; p = 0.73). There were no severe flares in either group. The results were similar after adjusting for length of SLE, number of American College of Rheumatology criteria, low complement levels, and SELENA-SLEDAI score, and in a propensity score analysis (OR = 1.18; 95% CI: 0.23, 6.16, p = 0.84). The analysis of time to any flare revealed a non-significant earlier time to flare in the HCQ withdrawal group (log rank p = 0.67). Most flares were in the cutaneous and musculoskeletal systems, but one patient in the continuation group developed pericarditis. The most common reason for HCQ withdrawal was maculopathy.Conclusions: In this retrospective study of older stable patients with SLE on long-term HCQ, withdrawal did not significantly increase the risk of flares.
Gelactin-9 Mediates the Therapeutic Effect of Mesenchymal Stem Cells on Experimental Endotoxemia
Authors: Zhao Yiming , Yu Dingding , Wang Hongda , Jin Wang , Li Xiang , Hu Yonghao , Qin Yafei , Kong Dejun , Li Guangming , Ellen Acheampong , Wang Hao 
Published: June 2020

Abstract
Background: Endotoxemia, mediated by uncontrolled immunocytes activation toward Lipopolysaccharide, could deteriorate into severe septic shock, but with limited treatment effect. Mesenchymal stem cells (MSCs), with excellent immune regulatory capacities, have displayed potential in multiple inflammatory disease treatment. Gelactin-9 (Gal-9), a newly discovered immune checkpoint, has been demonstrated to mediate immunomodulatory effect of MSCs in vitro. However, its in vivo role in alleviating endotoxemia remains to be elucidated.Methods: MSCs (2.5×10⁵/ml) were obtained and stimulated with IFN-γ (20ng/ml) for 72 hours. Gal-9 expression on MSCs were measured by ELISA, RT-PCR, flow cytometry and immunofluorescence respectively. Then, experimental endotoxemia was induced by LPS injection (10mg/kg, i.p.), followed by the treatment with Gal-9-MSC (20ng/ml, 72 hours), MSCs and MSC+α-lactose (10.8mg/mL, 500ul, i.v.). Therapeutic effects of MSC-based treatments were assessed by monitoring murine sepsis score, survival rate, splenocyte proportion, phenotype polarization, inflammatory mediator levels and pathological manifestations. Furthermore, Gal-9 expression in multiple organs was also detected after administering the treatments.Results: It has been found that MSCs expressed Gal-9 and its level was increased in a dose-dependent manner after being stimulated by IFN-γ. Moreover, adoptive transferred of IFN-γ pre-stimulated MSCs into endotoxemia mice was found with relieved symptoms and increased survival rate. Flow cytometry analysis indicated that Gal-9-MSC could promote macrophage polarization to M2-subtype and increase Treg ratios in spleen. Further results also demonstrated that, Gal-9-mediated MSC therapy could assist in attenuating local and circulating pro-inflammatory mediators expression (TNF-α, IL-1β, IFN-γ and iNOS), but increasing anti-inflammatory mediators expression (T-SOD and IL-35). Additionally, after administrating Gal-9-MSC, it was also found there was a significant relief in pathological manifestations, and with a higher expression of Gal-9 in liver, kidney and lung homogenate.Conclusions: This study revealed that Gal-9 mediated therapeutic effects of MSCs in alleviating endotoxemia injury, which provides a novel idea for supplementing the research of MSC immunoregulatory mechanism, and offers an excellent candidate to be used in treatment of endotoxemia in the clinical settings.