bluuebunny/crossref_metadata_2025_split huggingface dataset:
IMDAA Regional Reanalysis: Performance Evaluation During Indian Summer Monsoon Season
Authors: Ashrit Raghavendra , Indira Rani S. , Kumar Sushant , Karunasagar S. , Arulalan T. , Francis Timmy , Routray Ashish , Laskar S. I. , Mahmood Sana , Jermey Peter , Maycock Adam , Renshaw Richard , George John P. , Rajagopal E. N. 
Published: January 2020

AbstractThe Indian Monsoon Data Assimilation and Analysis (IMDAA) is a regional high‐resolution atmospheric reanalysis over the Indian subcontinent. This regional reanalysis over India is the first of its kind and is produced by the National Centre for Medium Range Weather Forecasting and Met Office, UK, in collaboration with the India Meteorological Department under the National Monsoon Mission project of the Ministry of Earth Sciences, Government of India. The reanalysis runs from 1979 to 2018, to span the era of modern meteorological satellites. This article briefly describes the IMDAA system and discusses the performance of the IMDAA during summer monsoon (June–September). This study provides evidence for substantial improvements seen in IMDAA compared to the ERA‐Interim reanalysis fields over India. The evaluation is carried out for the period of 1979–1993 for all major features associated with the Indian Monsoon to highlight improvements compared to ERA‐Interim and to document the biases. The study also demonstrates the potential use of the IMDAA data for applications such as wind resource assessment over India.
The Role of Ice Splintering on Microphysics of Deep Convective Clouds Forming Under Different Aerosol Conditions: Simulations Using the Model With Spectral Bin Microphysics
Authors: Qu Yi , Khain Alexander , Phillips Vaughan , Ilotoviz Eyal , Shpund Jacob , Patade Sachin , Chen Baojun 
Published: January 2020

AbstractObservations during the Ice in Clouds Experiment‐Tropical (ICE‐T) field experiment show that the ice particles concentration in a developing deep convective clouds at the level of T = −15 °C reached about 500 L−1, that is, many orders higher than that of ice‐nucleating particle. To simulate microphysics of these clouds, the 2‐D Hebrew University Cloud model (HUCM) with spectral bin microphysics was used in which two main types of ice multiplication mechanisms were included in addition to the Hallet‐Mossop mechanism. In the first ice multiplication mechanism ice splinters form by drop freezing and drop‐ice collisions. Ice multiplication of this type dominates during developing stage of cloud evolution, when liquid water content is significant. At later stage when clouds become nearly glaciated, ice crystals are produced largely by ice splintering during ice‐ice collisions (the second ice multiplication mechanism). Simulations show that droplet size distributions, as well as size distributions of ice particles, agree well with the measurements during ICE‐T. Simulations with different cloud condensation nuclei concentrations show the existence of the “optimum” cloud condensation nuclei concentration (or droplet concentration), at which concentration of ice splinters reaches maximum. In these simulations ice nucleation caused by the direct formation of ice crystals upon ice‐nucleating particles, as well as the Hallett‐Mossop process, has a negligible contribution to the ice crystal concentration.
A Mathematical Model for the Release, Transport, and Retention of Per‐ and Polyfluoroalkyl Substances (PFAS) in the Vadose Zone
Authors: Guo Bo , Zeng Jicai , Brusseau Mark L. 
Published: January 2020

AbstractPer‐ and polyfluoroalkyl substances (PFAS) are emerging contaminants of critical concern. As surfactants, PFAS tend to accumulate at air‐water interfaces and may stay in the vadose zone for long times before contaminating groundwater. Yet not well understood, the extent of retention in the vadose zone has critical implications for risk management and remediation strategies. We present the first mathematical model that accounts for surfactant‐induced flow and solid‐phase and air‐water interfacial adsorption. We apply the model to simulate PFOS (a PFAS compound of primary concern) transport in the vadose zone at a model fire‐training area site impacted by aqueous film‐forming foam (AFFF). Air‐water interfacial adsorption is shown to have a significant impact—amplified by the low water content due to gravity drainage—total retardation factors range from 233 to 1,355 for the sand and 146 to 792 for the soil used in the study. The simulations illustrate it can take several decades or longer for PFOS to reach groundwater. Counterintuitively, the lower water content in the sand—due to stronger drainage and weaker capillary retention—leads to retardation factors greater than for the soil. Also, most PFOS is adsorbed at air‐water interfaces with only 1–2% in the aqueous phase. The implications include (1) fine‐texture materials could have lower retardation factors than sand due to higher retained water content, (2) soil PFAS concentrations are likely to be orders of magnitude higher than those in groundwater at source zones. Both implications are consistent with recent field observations at hundreds of AFFF‐impacted sites.
Efficacy and safety of FOLFIRINOX as salvage treatment in advanced biliary tract cancer: an open-label, single arm, phase 2 trial
Authors: Belkouz Ali , de Vos-Geelen Judith , Mathôt Ron A. A. , Eskens Ferry A. L. M. , van Gulik Thomas M. , van Oijen Martijn G. H. , Punt Cornelis J. A. , Wilmink Johanna W. , Klümpen Heinz-Josef 
Published: January 2020

AbstractBackgroundNo standard treatment is available for advanced biliary tract cancer (BTC) after first-line therapy with gemcitabine plus cisplatin (GEMCIS). The objective of this study was to evaluate safety and anti-tumour activity of fluorouracil, leucovorin, irinotecan plus oxaliplatin (FOLFIRINOX) as salvage treatment in patients with previously treated advanced BTC.MethodsIn this two-stage phase 2 study, patients with advanced BTC who had disease progression or unacceptable toxicity after ≥3 cycles of GEMCIS were eligible. Primary endpoints were safety and efficacy (defined as objective response rate, ORR). In stage one, ten patients were treated with FOLFIRINOX every 2 weeks. In stage two, an additional 20 patients were enrolled at a starting dose as defined in stage one, provided that in stage ≥1 objective response or ≥2 stable diseases were observed and ≤3 patients had serious adverse events (SAEs) within the first 6 weeks of treatment. Secondary endpoints were progression-free survival (PFS) and overall survival (OS).ResultsForty patients were screened for eligibility and 30 patients were enrolled. In stage one, one patient had a partial response and five patients had stable disease. One patient had a SAE during the first 6 weeks of treatment, and five patients required a dose reduction due to adverse events. The most common grade 3–4 adverse events in stage one were neutropaenia, mucositis and diarrhoea. Stage two was initiated with FOLFIRINOX in an adapted dose. In stage two, grade 3–4 neutropaenia, diarrhoea, nausea and vomiting were the most common adverse events. The ORR, median PFS and OS in all patients were 10%, 6.2 and 10.7 months, respectively.ConclusionsIn patients with advanced BTC who progressed after or were intolerant to GEMCIS, FOLFIRINOX can be administered safely and could be considered as an option for salvage treatment in these patients.Clinical trial registrationClinicalTrials.gov Identifier NCT02456714.
MiR-107 confers chemoresistance to colorectal cancer by targeting calcium-binding protein 39
Authors: Liang Yu , Zhu Danxi , Hou Lidan , Wang Yu , Huang Xin , Zhou Cui , Zhu Liming , Wang Yingying , Li Lei , Gu Yan , Luo Meng , Wang Jianhua , Meng Xiangjun 
Published: January 2020

Abstract
Background
Chemoresistance remains a critical event that accounts for colorectal cancer (CRC) lethality. The aim of this study is to explore the ability of dichloroacetate (DCA) to increase chemosensitivity in CRC and the molecular mechanisms involved.

Methods
The effects of combination treatment of DCA and oxaliplatin (L-OHP) were analysed both in vitro and in vivo. The DCA-responsive proteins in AMPK pathway were enriched using proteomic profiling technology. The effect of DCA on CAB39–AMPK signal pathway was analysed. In addition, miRNA expression profiles after DCA treatment were determined. The DCA-responsive miRNAs that target CAB39 were assayed. Alterations of CAB39 and miR-107 expression were performed both in vitro and on xenograft models to identify miR-107 that targets CAB39–AMPK–mTOR signalling pathway.

Results
DCA increased L-OHP chemosensitivity both in vivo and in vitro. DCA could upregulate CAB39 expression, which activates the AMPK/mTOR signalling pathway. CAB39 was confirmed to be a direct target of miR-107 regulated by DCA. Alterations of miR-107 expression were correlated with chemoresistance development in CRC both in vitro and in vivo.

Conclusion
These findings suggest that the miR-107 induces chemoresistance through CAB39–AMPK–mTOR pathway in CRC cells, thus providing a promising target for overcoming chemoresistance in CRC.
Loss of PARP-1 attenuates diabetic arteriosclerotic calcification via Stat1/Runx2 axis
Authors: Li Peng , Wang Ying , Liu Xue , Liu Bin , Wang Zhao-yang , Xie Fei , Qiao Wen , Liang Er-shun , Lu Qing-hua , Zhang Ming-xiang 
Published: January 2020

AbstractAccelerated atherosclerotic calcification is responsible for plaque burden, especially in diabetes. The regulatory mechanism for atherosclerotic calcification in diabetes is poorly characterized. Here we show that deletion of PARP-1, a main enzyme in diverse metabolic complications, attenuates diabetic atherosclerotic calcification and decreases vessel stiffening in mice through Runx2 suppression. Specifically, PARP-1 deficiency reduces diabetic arteriosclerotic calcification by regulating Stat1-mediated synthetic phenotype switching of vascular smooth muscle cells and macrophage polarization. Meanwhile, both vascular smooth muscle cells and macrophages manifested osteogenic differentiation in osteogenic media, which was attenuated by PARP-1/Stat1 inhibition. Notably, Stat1 acts as a positive transcription factor by directly binding to the promoter of Runx2 and promoting atherosclerotic calcification in diabetes. Our results identify a new function of PARP-1, in which metabolism disturbance-related stimuli activate the Runx2 expression mediated by Stat1 transcription to facilitate diabetic arteriosclerotic calcification. PARP-1 inhibition may therefore represent a useful therapy for this challenging complication.
Antisymmetric linear magnetoresistance and the planar Hall effect
Authors: Wang Yishu , Lee Patrick A. , Silevitch D. M. , Gomez F. , Cooper S. E. , Ren Y. , Yan J.-Q. , Mandrus D. , Rosenbaum T. F. , Feng Yejun 
Published: January 2020

AbstractThe phenomena of antisymmetric magnetoresistance and the planar Hall effect are deeply entwined with ferromagnetism. The intrinsic magnetization of the ordered state permits these unusual and rarely observed manifestations of Onsager’s theorem when time reversal symmetry is broken at zero applied field. Here we study two classes of ferromagnetic materials, rare-earth magnets with high intrinsic coercivity and antiferromagnetic pyrochlores with strongly-pinned ferromagnetic domain walls, which both exhibit antisymmetric magnetoresistive behavior. By mapping out the peculiar angular variation of the antisymmetric galvanomagnetic response with respect to the relative alignments of the magnetization, magnetic field, and electrical current, we experimentally distinguish two distinct underlying microscopic mechanisms: namely, spin-dependent scattering of a Zeeman-shifted Fermi surface and anomalous electron velocities. Our work demonstrates that the anomalous electron velocity physics typically associated with the anomalous Hall effect is prevalent beyond the ρxy(Hz) channel, and should be understood as a part of the general galvanomagnetic behavior.
Heterologous viral protein interactions within licensed seasonal influenza virus vaccines
Authors: Koroleva Marina , Batarse Frances , Moritzky Savannah , Henry Carole , Chaves Francisco , Wilson Patrick , Krammer Florian , Richards Katherine , Sant Andrea J. 
Published: January 2020

AbstractCurrently, licensed influenza virus vaccines are designed and tested only for their ability to elicit hemagglutinin (HA)-reactive, neutralizing antibodies. Despite this, the purification process in vaccine manufacturing often does not completely remove other virion components. In the studies reported here, we have examined the viral protein composition of a panel of licensed vaccines from different manufacturers and licensed in different years. Using western blotting, we found that, beyond HA proteins, there are detectable quantities of neuraminidase (NA), nucleoprotein (NP), and matrix proteins (M1) from both influenza A and influenza B viruses in the vaccines but that the composition differed by source and method of vaccine preparation. We also found that disparities in viral protein composition were associated with distinct patterns of elicited antibody specificities. Strikingly, our studies also revealed that many viral proteins contained in the vaccine form heterologous complexes. When H1 proteins were isolated by immunoprecipitation, NA (N1), M1 (M1-A), H3, and HA-B proteins were co-isolated with the H1. Further biochemical studies suggest that these interactions persist for at least 4 h at 37 °C and that the membrane/intracytoplasmic domains in the intact HA proteins are important for the intermolecular interactions detected. These studies indicate that, if such interactions persist after vaccines reach the draining lymph node, both dendritic cells and HA-specific B cells may take up multiple viral proteins simultaneously. Whether these interactions are beneficial or harmful to the developing immune response will depend on the functional potential of the elicited virus-specific CD4 T cells.
Label-free impedance flow cytometry for nanotoxicity screening
Authors: Ostermann Melanie , Sauter Alexander , Xue Ying , Birkeland Eivind , Schoelermann Julia , Holst Bodil , Cimpan Mihaela Roxana 
Published: January 2020

AbstractThe development of reliable and cost-efficient methods to assess the toxicity of nanomaterials (NMs) is critical for the proper identification of their impact on human health and for ensuring a safe progress of nanotechnology. In this study, we investigated the reliability and applicability of label-free impedance flow cytometry (IFC) for in vitro nanotoxicity screening, which avoids time-consuming labelling steps and minimizes possible NM-induced interferences. U937 human lymphoma cells were exposed for 24 h to eight different nanomaterials at five concentrations (2, 10, 20, 50, and 100 μg/mL). The NMs’ effect on viability was measured using IFC and the results were compared to those obtained by trypan blue (TB) dye exclusion and conventional flow cytometry (FC). To discriminate viable from necrotic cells, the IFC measurement settings regarding signal trigger level and frequency, as well as the buffer composition, were optimised. A clear discrimination between viable and necrotic cells was obtained at 6 MHz in a sucrose-based measurement buffer. Nanomaterial-induced interferences were not detected for IFC. The IFC and TB assay results were in accordance for all NMs. The IFC was found to be robust, reliable and less prone to interferences due to the advantage of being label-free.
Transmissibility of acute haemorrhagic conjunctivitis in small-scale outbreaks in Hunan Province, China
Authors: Zhang Siyu , Hu Qingqing , Deng Zhihong , Hu Shixiong , Liu Fuqiang , Yu Shanshan , Liu Ruoyun , He Chunlei , Li Hongye , Gao Lidong , Chen Tianmu 
Published: January 2020

AbstractAcute haemorrhagic conjunctivitis (AHC) outbreaks are reported frequently in China. However, the transmissibility of AHC remains unclear. This study aimed to calculate the transmissibility of the disease with and without interventions. An AHC outbreak dataset from January 2007 to December 2016 in different schools was built in Hunan Province. A Susceptible-Infectious-Recovered (SIR) model was adopted to calculate the effective reproduction number (Reff) of AHC. Reff was divided into two parts (Runc and Rcon) where Runc and Rcon represent the uncontrolled and controlled Reff , respectively. Based on Runc and Rcon, an index of effectiveness of countermeasures (Ieff) was developed to assess the effectiveness of countermeasures in each outbreak. During the study period, 34 AHC outbreaks were reported in 20 counties of 9 cities in Hunan Province, with a mean total attack rate of 7.04% (95% CI: 4.97–9.11%). The mean Runc of AHC outbreaks was 8.28 (95% CI: 6.46–10.11). No significance of Runc was observed between rural and urban areas (t = −1.296, P = 0.205), among college, secondary, and primary schools (F = 0.890, P = 0.459), different levels of school population (F = 0.738, P = 0.538), and different number of index cases (F = 1.749, P = 0.180). The most commonly implemented countermeasures were case isolation, treatment, and health education, followed by environment disinfection, symptom surveillance, and school closure. Social distance, prophylaxis, and stopping eye exercises temporary were implemented occasionally. The mean value of Rcon was 0.16 (range: 0.00–1.50). The mean value of Ieff was 97.16% (range: 71.44–100.00%). The transmissibility of AHC is high in small-scale outbreaks in China. Case isolation, treatment, and health education are the common countermeasures for controlling the disease.
25-vitamin D reduces inflammation in uremic environment
Authors: Brito Rodrigo Barbosa de Oliveira , Rebello Jacqueline Ferritto , Grabulosa Caren Cristina , Pinto Walter , Morales Armando , Elias Rosilene Motta , Moyses Rosa Maria Affonso , Dalboni Maria Aparecida 
Published: January 2020

AbstractChronic kidney disease (CKD) is characterized by loss of renal function and a consequent increase of serum uremic toxins, which contribute to inflammation status. Deficiency of 25-vitamin D, often found in patients with CKD, has been included as an inflammatory factor since it might modulate the immune system. The aim of this study was to investigate the role of 25-vitamin D on inflammatory pathways in healthy and uremic environment. Toll-like receptor 4 (TLR4), oxidative stress (ROS), vitamin D receptor (VDR), 1-α hydroxylase (CYP27), 24 hydroxylase, cathelicidin, and MCP-1 were evaluated in monocytes exposed to a uremic serum pool compared with healthy pool. The human monocytes lineage (U937) was incubated with or without 25-vitamin D (50 ng/ml for 24 hours). TRL4, VDR, CYP27, CYP24, and ROS were evaluated by flow cytometry. We used ELISA to measure IL-6, TNF-α, IL-10, cathelicidin, and MCP-1 in the cell culture supernatant. We observed a higher expression of TRL-4, IL-6, TNF-α, IL-10, cathelicidin and MCP-1 in monocytes incubated with uremic serum when compared with serum from healthy individuals. Supplementation of 25-vitamin D was able to reduce the expression of TRL4, cathelicidin, and MCP-1 in the uremic environment. There was no difference in the expression of VDR, CYP27 and CYP24 intracellular enzymes. This in vitro study showed that the uremic pool activates inflammatory response in monocytes, which was reversed by 25-vitamin D supplementation; this finding suggests that 25-vitamin D has an anti-inflammatory role in the uremic environment.
Machine-learning facilitates selection of a novel diagnostic panel of metabolites for the detection of heart failure
Authors: Marcinkiewicz-Siemion M. , Kaminski M. , Ciborowski M. , Ptaszynska-Kopczynska K. , Szpakowicz A. , Lisowska A. , Jasiewicz M. , Tarasiuk E. , Kretowski A. , Sobkowicz B. , Kaminski K. A. 
Published: January 2020

AbstractThe metabolic derangement is common in heart failure with reduced ejection fraction (HFrEF). The aim of the study was to check feasibility of the combined approach of untargeted metabolomics and machine learning to create a simple and potentially clinically useful diagnostic panel for HFrEF. The study included 67 chronic HFrEF patients (left ventricular ejection fraction-LVEF 24.3 ± 5.9%) and 39 controls without the disease. Fasting serum samples were fingerprinted by liquid chromatography-mass spectrometry. Feature selection based on random-forest models fitted to resampled data and followed by linear modelling, resulted in selection of eight metabolites (uric acid, two isomers of LPC 18:2, LPC 20:1, deoxycholic acid, docosahexaenoic acid and one unknown metabolite), demonstrating their predictive value in HFrEF. The accuracy of a model based on metabolites panel was comparable to BNP (0.85 vs 0.82), as verified on the test set. Selected metabolites correlated with clinical, echocardiographic and functional parameters. The combination of two innovative tools (metabolomics and machine-learning methods), both unrestrained by the gaps in the current knowledge, enables identification of a novel diagnostic panel. Its diagnostic value seems to be comparable to BNP. Large scale, multi-center studies using validated targeted methods are crucial to confirm clinical utility of proposed markers.
Physicochemical properties, microstructures, nutritional components, and free amino acids of Pleurotus eryngii as affected by different drying methods
Authors: Yang Rui-Lin , Li Qin , Hu Qing-Ping 
Published: January 2020

AbstractIn this study, we determined the influences of different drying techniques such as natural air (ND), hot-air (HD), vacuum (VD), infrared (ID), microwave (MD), and freeze drying (FD) methods on the color, shrinkage ratio (SR), rehydration ratio (RR), firmness, crispness, microstructures, nutritional components, and free amino acids of Pleurotus eryngii. The results showed that these parameters were markedly influenced by different drying techniques. Among them, FD was the most effective drying method which retained the main characteristics of the fresh P. eryngii in above mentioned indexes, followed by ND and HD at 40 °C. Finally, despite the least drying time, MD treatment was not suitable to the drying process of P. eryngii slices since it damaged physicochemical properties and caused massive losses of the main nutrients and free amino acids. The results will provide a theoretical basis for industrial processing of P. eryngii.
Faces Divulge the Origins of Caribbean Prehistoric Inhabitants
Authors: Ross Ann H. , Keegan William F. , Pateman Michael P. , Young Colleen B. 
Published: January 2020

AbstractThe origins of the first peoples to colonize the Caribbean Islands have been the subject of intense debate for over 30 years. Competing hypotheses have identified five separate migrations from the mainland with a separate debate concerning the colonization of The Bahamas. Significant differences in the facial morphology of the pre-Columbian inhabitants of Hispaniola and Cuba led to the present study of Lucayan skulls from The Bahamas. The goal was to determine which group the native Lucayans more closely resembled to resolve this long-standing dispute. The results indicate that they are related to groups from Hispaniola and Jamaica and not to Cuban inhabitants. This study clarified the larger picture of Caribbean migrations and supports evidence for a Carib invasion of the Greater Antilles around AD 800.