bluuebunny/crossref_metadata_2025_split huggingface dataset:
Chronic kidney disease and acute kidney injury in the COVID-19 Spanish outbreak
Authors: Portolés Jose , Marques María , López-Sánchez Paula , de Valdenebro María , Muñez Elena , Serrano María Luisa , Malo Rosa , García Estefanya , Cuervas Valentín 
Published: June 2020

AbstractBackgroundNovel coronavirus disease 2019 (COVID-19) emerged in Wuhan and rapidly spread, affecting &gt;10 million cases worldwide. Caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and primarily manifesting as an acute respiratory failure with interstitial and alveolar pneumonia, it can also affect multiple organs. Kidney involvement was underestimated in early reports and its role remains controversial. The aim of this study was to analyse the role of kidney damage in COVID-19 outcome.MethodsThis is a prospective cohort study of 1603 consecutive patients admitted in a University Reference Hospital in the heart of the European outbreak.ResultsMedian age was 64 years, 40.4% were female, 15.2% presented diabetes mellitus, 35.7% hypertension and 20.3% obesity. On admission, the prevalence of elevated serum creatinine (sCr), proteinuria, leucocyturia and haematuria were 21.0, 37.8, 31.8 and 45.6%, respectively. In total, 43.5% of those with an elevated sCr had previous chronic kidney disease (CKD) and 11.4% of those with normal sCr developed an in-hospital acute kidney injury (AKI); 17 patients needed acute haemodialysis; and 197 patients died during hospitalization. Cox proportional hazard regression confirmed that elevated baseline sCr [hazard ratio (95% confidence interval) 2.40 (1.79–3.22)], previous CKD [1.59 (1.06-2.37)], haematuria [1 + 1.68 (0.92–3.06), 2–3 + 2.69 (1.49–4.87)] and in-hospital AKI [1.50 (0.92–2.44)] were independent risk factors for in-hospital death after adjusting for age, sex and comorbidity.ConclusionThe prevalence of acute and chronic kidney disease on admission and in-hospital AKI is higher than previously reported in Wuhan, and is associated with high in-hospital mortality. We should increase our awareness towards kidney involvement and design specific strategies for management of COVID-19 in these patients.
Effect of Physical Exercise Programs on Myofascial Trigger Points–Related Dysfunctions: A Systematic Review and Meta-analysis
Authors: Guzmán-Pavón María José , Cavero-Redondo Iván , Martínez-Vizcaíno Vicente , Fernández-Rodríguez Rubén , Reina-Gutierrez Sara , Álvarez-Bueno Celia 
Published: June 2020

AbstractObjectiveMyofascial pain syndrome is one of the primary causes of health care visits. In recent years, physical exercise programs have been developed for the treatment of myofascial trigger points, but their effect on different outcomes has not been clarified. Thus, this study aimed to assess the effect of physical exercise programs on myofascial trigger points.MethodsA systematic search was conducted in Pubmed, Web of Science, and Scopus. Articles analyzing the effect of physical exercise programs on pain intensity, pressure pain threshold, range of motion, and disability were included. Risk of bias was assessed using the Cochrane RoB2 tool. The DerSimonian-Laird method was used to compute the pooled effect sizes (ES) and their 95% confidence interval (95% CI) for pain intensity, pressure pain threshold, range of motion, and disability.ResultsA total of 24 randomized controlled trials were included in this systematic review and meta-analysis. The pooled ES were –0.47 (95% CI = –0.61 to –0.33) for pain intensity, 0.63 (95% CI = 0.31 to 0.95) for pressure pain threshold, 0.43 (95% CI = 0.24 to 0.62) for range of motion, and –0.18 (95% CI = –0.45 to 0.10) for disability.ConclusionsPhysical exercise programs may be an effective approach in the treatment of pain intensity, pressure pain threshold, and range of motion among patients with myofascial trigger points.
How Efficient are Decentralized Auction Platforms?
Authors: Bodoh-Creed Aaron L , Boehnke Jörn , Hickman Brent 
Published: June 2020

Abstract
We model a decentralized, dynamic auction market platform in which a continuum of buyers and sellers participate in simultaneous, single-unit auctions each period. Our model accounts for the endogenous entry of agents and the impact of intertemporal optimization on bids. We estimate the structural primitives of our model using Kindle sales on eBay. We find that just over one-third of Kindle auctions on eBay result in an inefficient allocation with deadweight loss amounting to 14% of total possible market surplus. We also find that partial centralization—for example, running half as many 2-unit, uniform-price auctions each day—would eliminate a large fraction of the inefficiency, but yield lower seller revenues. Our results also highlight the importance of understanding platform composition effects—selection of agents into the market—in assessing the implications of market redesign. We also prove that the equilibrium of our model with a continuum of buyers and sellers is an approximate equilibrium of the analogous model with a finite number of agents.
The Negative Association between Alternative Financial Services Usage and Financial Well-Being: Variations by Income
Authors: Chen Zibei , Livermore Michelle 
Published: June 2020

Abstract
As reliance on alternative financial services (AFS) usage continues its exponential expansion among American families, policy debates over banking regulation perdure with limited empirical understanding of how usage affects individuals’ financial lives. Using data from the 2014 Survey of Household Economics and Decisionmaking, this study explored the association between AFS use and financial well-being using a nationally representative sample (N = 5,896). It also examined the role of household income in AFS use and its relation to financial well-being. Results from regression analyses indicated that AFS use was negatively associated with present financial security measured by credit score, making ends meet, subjective financial well-being, and credit card payment, and that future financial security was measured by having an emergency fund and a rainy-day fund. Moreover, the interaction models showed that lower-income groups had the most negative associations between AFS and most financial well-being indicators, suggesting a substantive role of income in exacerbating the negative relationships. This was the first known study linking use of AFS and household financial well-being with a focus on the role of income. The article concludes with a discussion of implications for policy and social work practice.
Remdesivir Receives Emergency Use Authorization for Severely Ill Patients with COVID-19
Authors: Aschenbrenner Diane S. 
Published: June 2020

The antiviral remdesivir has received Food and Drug Administration (FDA) Emergency Use Authorization to treat patients severely ill with COVID-19. The drug is still considered unapproved and under investigation.Nurses should review the Fact Sheet for Health Care Providers for full information regarding reconstitution and administration of remdesivir. Health care providers should report all adverse events and medication errors to the FDA MedWatch program and to Gilead, the drug's manufacturer.
Preventing Falls Among Behavioral Health Patients
Authors: Ocker Stephanie Ann , Barton Sandra A. , Bollinger Norma , Leaver Cynthia A. , Harne-Britner Sarah , Heuston Melanie M. 
Published: June 2020

ABSTRACT

Background:
Inpatient falls are among the most common hospital incidents reported. Fall-related injuries have significant implications for patients, staff, and organizations. Adult behavioral health inpatients are responsible for higher rates of injurious falls and challenge traditional fall prevention methods. An inpatient behavioral health unit in an acute care hospital identified an increased rate of injury from falls per 1,000 patient-days in three months; three falls resulted in major injury.

Purpose:
The purpose of this quality improvement (QI) report is to describe the redesign of a fall prevention program for adult behavioral health inpatients using evidence-based practice (EBP) and QI methods.

Methods:
Root cause analyses (RCAs) were conducted on all three fall-related major injuries. Concurrently, a literature review identified EBP recommendations for fall prevention on behavioral health units. A fall prevention action program was developed consisting of four elements: RN education to improve Morse Fall Scale scoring, individualized fall prevention patient plans, revised staff workflow, and improved fall prevention communication.

Results:
There were no fall-related injuries for six months and no fall-related major injuries for 12 months following implementation of the fall prevention program. Overall, this reduction in fall-related major injuries was sustained over a 21-month period.

Conclusion:
An interprofessional team approach using EBP and RCA is effective in redesigning and implementing a fall prevention program for the adult inpatient behavioral health population.
Matrix Metalloproteinase 11 as a Novel Tumor Promoter and Diagnostic and Prognostic Biomarker for Pancreatic Ductal Adenocarcinoma
Authors: Zhang Xu , Lu Jun , Zhou Li , You Lei , Liang Zhiyong , Guo Junchao , Zhao Yupei 
Published: June 2020

Objectives
Matrix metalloproteinase 11 (MMP-11) was found to be implicated in tumorigenesis in cancers. However, the significance of MMP-11 in pancreatic ductal adenocarcinoma (PDAC) is unclear.

Methods
In the study, we detected malignant biological behaviors of pancreatic cancer after downregulation of MMP-11. Furthermore, we explored the possible mechanism, and the diagnostic value of serum MMP-11 level was analyzed in 116 patients with pathologically confirmed PDAC. In addition, we explored their prognostic value in PDAC.

Results
We observed that MMP-11 could be expressed and activated in the cytoplasm of PDAC cells. Immunohistochemistry staining of PDAC tissues showed that MMP-11 was highly expressed in cancerous ductal epithelium instead of cancer stroma. We found that downregulation of MMP-11 inhibited proliferation of PDAC cell lines. The expression levels of cyclin-dependent kinase 4 and cyclin D1 were downregulated after MMP-11 knockdown. As for its clinical value, the serum level of MMP-11 was shown to be a potent promising diagnostic marker for PDAC.

Conclusions
Matrix metalloproteinase 11 may act as a tumor promoter, playing a positive role in PDAC development. Serum MMP-11 also has great potential to be a promising diagnostic marker for PDAC.
Hydrogen Gas Alleviates Sepsis-Induced Brain Injury by Improving Mitochondrial Biogenesis Through the Activation of PGC-α in Mice
Authors: Xie Keliang , Wang Yaoqi , Yin Lijun , Wang Yuzun , Chen Hongguang , Mao Xing , Wang Guolin 
Published: June 2020

ABSTRACT
Sepsis-associated encephalopathy (SAE) affects approximately one-third of septic patients, and there is a lack of effective therapeutics for SAE. Hydrogen gas is a new medical gas that exerts anti-inflammation, antioxidation, and anti-apoptotic effects and can effectively protect septic mice. Mitochondrial dysfunction, which can be improved by mitochondrial biogenesis, is a type of molecular pathology in sepsis. Peroxisome proliferator-activated receptor gamma co-activator 1α (PGC-1α), which can be inhibited by SR-18292, is the key regulatory factor of mitochondrial biogenesis. Therefore, we investigated the effects of hydrogen gas on mitochondrial function and mitochondrial biogenesis in mice with SAE and the related regulatory mechanisms. Cecal ligation and puncture was used to induce sepsis in mice. The mice with hydrogen gas therapy were exposed to 2% H2 inhalation for 1 h beginning at both 1 and 6 h after operation, and mice were also injected with a PGC-1α inhibitor, SR-18292. We recorded the 7-day survival rates of the mice and detected their cognitive function using a Y-maze test. The Nissl bodies in the CA1 region of hippocampus were observed by Nissl staining, and the apoptotic cells were observed by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling assay staining. The mitochondrial membrane potential (MMP), adenosine triphosphate (ATP) level, and mitochondrial respiratory chain complexes I and II were analyzed using commercial kits. The mitochondrial morphology was observed by transmission electron microscopy. The expression levels of PGC-1α, nuclear respiratory factor 2 (NRF2), and mitochondrial transcription factor A (Tfam) were detected by Western blot analysis. The present study showed that hydrogen gas therapy increased the 7-day survival rate, improved cognitive function, increased the mitochondrial function (MMP, ATP level, complex I activity) and expression of mitochondrial biogenesis parameters (PGC-1α, NRF2, Tfam). However, the injection of SR-18292 (a PGC-1α inhibitor) decreased mitochondrial function, PGC-1α activation, and expression of NRF2 and Tfam. Therefore, these results indicate that hydrogen gas alleviates sepsis-induced brain injury in mice by improving mitochondrial biogenesis through the activation of PGC-1α.
Temporal Dysregulation of the Angiopoietin-2/-1 Ratio After Trauma and Associations With Injury Characteristics and Outcomes
Authors: Uhlich Rindi M. , Richter Robert P. , Hu Parker J. , Kirkman Alyssa A. , Ashtekar Amit R. , Zheng Lei , Walker Shannon C. , Reynolds Lindy M. , Griffin Russell L. , Jansen Jan O. , Kerby Jeffrey D. , Richter Jillian R. 
Published: June 2020

ABSTRACT
Traumatic injury and hemorrhagic shock result in endothelial cell activation and vascular dysfunction that, if not corrected, can propagate multiorgan failure. Angiopoietin-1 and angiopoietin-2 are important regulators of endothelial cell function, and the ratio of plasma angiopoietin-2-to-1 is a useful indicator of overall vascular health. We therefore characterized plasma angiopoietin-2/-1 ratios over time after trauma in adults in an effort to gain insight into the pathophysiology that may drive post-traumatic vasculopathy and organ injury. We performed a single-center prospective observational study to measure plasma angiopoietin-1 and -2 levels and determine angiopoietin-2/-1 ratios in adult trauma patients upon hospital arrival and after 12, 24, and 48 h. Compared with levels in healthy adults, angiopoietin-1 levels were significantly elevated at hospital arrival, and angiopoietin-2 levels were significantly elevated at 12, 24, and 48 h. These kinetics translated in angiopoietin-2/-1 ratios that were significantly greater than controls at 24 and 48 h. After regression analysis, elevated angiopoietin-2 levels were independently associated with blunt injuries at admission, with coagulopathy at admission and 12 h, and with hemorrhagic shock at 24 and 48 h. Significant correlations were observed between both angiopoietins and 24-h transfusion requirements. Angiopoietin-2/-1 ratios correlated with mechanical ventilation duration and intensive care unit and hospital lengths of stay. In this study, we demonstrate novel temporal associations between angiopoietin dysregulation and blunt injuries, acute coagulopathy, and hemorrhagic shock. Moreover, our findings highlight the presence of endothelial activation following traumatic insults in adults that may contribute to worse clinical outcomes.
Human Lungs Airway Epithelium Upregulate MicroRNA-17 and MicroRNA-548b in Response to Cold Ischemia and Ex Vivo Reperfusion
Authors: Elgharably Haytham , Okamoto Toshihiro , Ayyat Kamal S. , Niikawa Hiromichi , Meade Sirena , Farver Carol , Chan E. Ricky , Aldred Micheala A. , McCurry Kenneth R. 
Published: June 2020

Background.
Lung ischemia-reperfusion injury after transplantation is associated with worse clinical outcomes. MicroRNA (miR) are critical regulators of gene expression that could provide potential targets for novel gene therapy. Herein, we aim to examine the feasibility of using the ex vivo lung perfusion (EVLP) platform to examine the changes in miR expression in human lungs in response to cold ischemia and ex vivo reperfusion (CI/EVR).

Methods.
Twenty-four human lungs were perfused in cellular EVLP system for 2 h, and tissue samples were obtained before and after EVLP as well as from control donors. MicroRNA expression profiling of the lung tissue was performed using next-generation sequencing and downstream predicted target genes were examined. In situ hybridization assay of the validated miR was used to identify the expressing cell type.

Results.
After 2 h of EVLP, cytokines production was significantly increased (IL-1β, IL-6, IL-8, IL-10, and TNF-α). MicroRNA sequencing identified a significant change in the expression of a total of 21 miR after CI and 47 miR after EVR. Validation using quantitative polymerase chain reaction showed significant upregulation of miR-17 and miR548b after CI/EVR. Downstream analysis identified abundant inflammatory and immunologic targets for miR-17 and miR-548b that are known mediators of lung injury. In situ hybridization assays detected positive signals of the 2 miR expression in alveolar epithelial cells.

Conclusions.
This study demonstrates the feasibility of using the EVLP platform to study miR signature in human lungs in response to CI/EVR. We found that miR-17 and miR-548b were upregulated in alveolar epithelial cells after CI/EVR, which merit further exploration.
The Knowledge, attitude and practices about bio-medical waste management system in a tertiary care 60 bedded hospital in Rajasthan, India
Authors: Verma Virender , Soni Priya , Kalhan Meenakshi , Nanda Sanjiv , Kumar Anjani , Nandan Vivek 
Published: June 2020

Background and PurposeFrom antiquity, the healthcare facilities have treated sick people but there was little awareness about the fact that hospitals generate a lot of hazardous bio-medical waste, which had been disposed without proper guidelines. Lately, it has been a proven thing that bio-medical-waste is a potential hazard for heath-personals, and environment. Therefore, it must be disposed in a proper manner.ObjectivesThis was an observational study carried out at one of the tertiary care hospitals in Rajgarh city about the knowledge, attitude and practices of the hospital staff about the bio-medical waste management.Material and methodAn observational (cross-sectional) study using questionnaires was carried out at one of the non-NABH accredited tertiary care hospitals of Rajgarh City in Rajasthan (India).ResultsMore than 70% staff (except housekeeping) had good ideas about waste categories, segregation and color coding except in radioactive waste. The house keeping staff did well in 3 categories (Linen, sharps and glasses). Most of the employees has clarity about BMW except the housekeeping staff which did well on most of parameters except barcoding, pretreatment of anatomical/ biotechnology waste, knowledge about STP plant (Sewage treatment plant), Hazmat and signage. On actual hospital rounds we found that the hazmat practices were in a poor shape. Most of staff was aware occupational hazards except housekeeping staff.ConclusionA written policy, induction training of healthcare workers, constant and repetitive workshops, and motivation are important human factors to implement the biomedical waste practices in a small-scale healthcare organization. Risk-stratification, understanding of health hazards and how to activate hazmat protocols are important things which are to be brought into practice.
Innocent until proven guilty: Privacy-preserving search over a central CODIS criminal database from the field
Authors: Blindenbach Jacob A. , Jagadeesh Karthik A. , Bejerano Gill , Wu David J. 
Published: June 2020

AbstractThe presumption of innocence (i.e., the principle that one is considered innocent until proven guilty) is a cornerstone of the criminal justice system in many countries, including the United States. DNA analysis is an important tool for criminal investigations1. In the U.S. alone, it has already aided in over half a million investigations using the Combined DNA Index System (CODIS) and associated DNA databases2. CODIS includes DNA profiles of crime scene forensic samples, convicted offenders, missing persons and more. The CODIS framework is currently used by over 50 other countries3including much of Europe, Canada, China and more. During investigations, DNA samples can be collected from multiple individuals who may have had access to, or were found near a crime scene, in the hope of finding a single criminal match4. Controversially, CODIS samples are sometimes retained from adults and juveniles despitenotyielding any database match4–6. Here we introduce a cryptographic algorithm that finds any and all matches of a person’s DNA profile against a CODIS databasewithoutrevealing anything about the person’s profile to the database provider. With our protocol, matches are immediately identified as before; however, individuals who donotmatch anything in the database retain their full privacy. Our novel algorithm runs in 40 seconds on a CODIS database of 1,000,000 entries, enabling its use to privately screen potentially-innocent suspects even in the field.
Mass-spectrometry-based near-complete draft of theSaccharomyces cerevisiae proteome
Authors: Gao Yuan , Ping Lingyan , Duong Duc , Zhang Chengpu , Dammer Eric B. , Li Yanchang , Chen Peiru , Chang Lei , Gao Huiying , Wu Junzhu , Xu Ping 
Published: June 2020

AbstractProteomics approaches designed to catalogue all open reading frames (ORFs) under a defined set of growth conditions of an organism have flourished in recent years. However, no proteome has been sequenced completely so far. Here we generate the largest yeast proteome dataset, including 5610 identified proteins using a strategy based on optimized sample preparation and high-resolution mass spectrometry. Among the 5610 identified proteins, 94.1% are core proteins, which achieves near complete coverage of the yeast ORFs. Comprehensive analysis of missing proteins in our dataset indicate that the MS-based proteome coverage has reached the ceiling. A review of protein abundance shows that our proteome encompasses a uniquely broad dynamic range. Additionally, these values highly correlate with mRNA abundance, implying a high level of accuracy, sensitivity and precision. We present examples of how the data could be used, including re-annotating gene localization, providing expression evidence of pseudogenes. Our near complete yeast proteome dataset will be a useful and important resource for further systematic studies.
3D Visualization of Macromolecule Synthesis
Authors: Duerr Timothy J. , Comellas Ester , Jeon Eun Kyung , Farkas Johanna E. , Joetzjer Marylou , Garnier Julien , Shefelbine Sandra J. , Monaghan James R. 
Published: June 2020

AbstractMeasuring nascent macromolecular synthesisin vivois key to understanding how cells and tissues progress through development and respond to external cues. Here, we performin vivoinjection of alkyne- or azide-modified analogs of thymidine, uridine, methionine, and glucosamine to label nascent synthesis of DNA, RNA, protein, and glycosylation. Three-dimensional volumetric imaging of nascent macromolecule synthesis was performed in axolotl salamander tissue using whole mount click chemistry-based fluorescent staining followed by light sheet fluorescent microscopy. We also developed an image processing pipeline for segmentation and classification of morphological regions of interest and individual cells, and we apply this pipeline to the regenerating humerus. We demonstrate our approach is sensitive to biological perturbations by measuring changes in DNA synthesis after limb denervation. This method provides a powerful means to quantitatively interrogate macromolecule synthesis in heterogenous tissues at the organ, cellular, and molecular levels of organization.