bluuebunny/crossref_metadata_2025_split huggingface dataset:
Neuroscience Perspectives on Cognitive Training
Authors: Kuchinsky Stefanie E. , Haarmann Henk J. 
Published: January 2020

Abstract
The aim of this chapter is to spark a discussion regarding how cognitive neuroscience research can aid in the evaluation and development of effective cognitive training protocols. In particular, the authors pose questions relating to whether training-related neural plasticity (i.e., changes in brain function and structure in response to experience) could be used to facilitate the identification and targeting of the neural systems (for working memory and other executive functions) that both support performance on a desired outcome task (e.g., speech recognition) and are alterable via training. The chapter describes approaches that provide unique methodological perspectives for understanding the neural systems that support training-related improvements in cognition. The chapter also highlights the multiple challenges that have emerged from behavioral studies of cognitive training and that neuroscience techniques may help to address, including: establishing the extent to which cognitive training benefits exist for trained tasks and materials, transfer to untrained tasks and materials, persist for extended periods of time, and are effective across a range of individuals. Cognitive neuroscience research has begun not only to tackle these challenges but also to pose new questions, such as: Can training benefits be maximized via regulating or stimulating the neural systems that support behavior? How might our current approaches to cognitive training be significantly altered by novel and developing cognitive neuroscience methodologies?
Working Memory Training and Transcranial Direct Current Stimulation
Authors: Au Jacky , Buschkuehl Martin , Jaeggi Susanne M. 
Published: January 2020

Abstract
The aim of this chapter is to contribute to the discussion of the cognitive neuroscience of brain stimulation. In doing so, the authors emphasize work from their own laboratory that focuses both on working memory training and transcranial direct current stimulation. Transcranial direct current stimulation is one of the most commonly used and extensively researched methods of transcranial electrical stimulation. The chapter focuses on implementation of transcranial direct current stimulation to enhance and inform research on working memory training, and not on the underlying mechanisms of transcranial direct current stimulation. Thus, while respecting the intricacies and unknowns of the inner workings of electrical stimulation on the brain, the chapter relies on the premise that transcranial direct current stimulation is able to directly affect the electrophysiological profile of the brain and provides evidence that this in turn can influence behavior given the right parameters.
Beyond Limits: Unmasking Potential Through Movement Discovery
Authors: Fisher Beth E 
Published: January 2020

Abstract
Beth E. Fisher, PT, PhD, FAPTA, is a physical therapist, educator, and scholar whose clinical career has been shaped by numerous observations and experiences of patients’ remarkable potential to recover ideal movement capability. Currently, Dr Fisher is a Professor of Clinical Physical Therapy in the Division of Biokinesiology and Physical Therapy at the University of Southern California. She is Director of the Neuroplasticity and Imaging Laboratory, primarily using transcranial magnetic stimulation to investigate brain-behavior relationships during motor skill learning and motor control in both individuals without disabilities and individuals with neurologic disorders. Dr Fisher previously worked at Rancho Los Amigos Medical Center on the Adult Neurology and Brain Injury Services. During her years as a clinician and rehabilitation specialist, it was her greatest ambition to be a part of developing physical therapist interventions that would maximize neural and behavioral recovery in individuals with pathological conditions affecting the nervous system. Toward this goal, she has continued to consult and teach nationally and internationally on current concepts for the treatment of adults with neurological disorders. It has been her consistent clinical observation not only that patients are limited by impairments that result from their injury, but that movement abnormalities are in part the result of a patient’s automatic, implicit tendency to “respond” to those impairments via compensation.
Brain networks mediating the influence of background music on selective attention
Authors: Fernandez Natalia B , Trost Wiebke J , Vuilleumier Patrik 
Published: January 2020

Abstract
Prevalent across societies and times, music has the ability to enhance attention, a property relevant to clinical applications, but the underlying brain mechanisms remain unknown. It is also unclear whether music produces similar or differential effects with advancing age. Here, we used event-related functional magnetic resonance imaging to investigate the influence of music exposure evoking four types of emotions on distinct attentional components measured with a modified attention network test, across 19 young (21 ± 2.6) and 33 old participants (72 ± 5.4). We then determined whether music-related effects differed across age groups and whether they were associated with particular acoustic features. Background music during selective attention requiring distractor conflict resolution was associated with faster response times and greater activations of fronto-parietal areas during happy and high-arousing music, whereas sad and low-valence music was associated with slower responses and greater occipital recruitment. Shifting and altering components of attention were unaffected. The influence of music on performance and brain networks was similar between age groups. These behavioral and neuroimaging results demonstrate the importance of affective music dimensions, particularly arousal, in enhancing selective attention processes. This study adds novel support to the benefits of music in the rehabilitation of attention functions.
A repository of web-based bioinformatics resources developed in India
Authors: Agarwal Abhishek , Agrawal Piyush , Sharma Aditi , Kumar Vinod , Mugdal Chirag , Dhall Anjali , Raghava Gajendra P.S. 
Published: January 2020

AbstractIndiaBioDb (https://webs.iiitd.edu.in/raghava/indiabiodb/) is a manually curated comprehensive repository of bioinformatics resources developed and maintained by Indian researchers. This repository maintains information about 543 freely accessible functional resources that include around 258 biological databases. Each entry provides a complete detail about a resource that includes the name of resources, web link, detail of publication, information about the corresponding author, name of institute, type of resource. A user-friendly searching module has been integrated, which allows users to search our repository on any field. In order to retrieve categorized information, we integrate the browsing facility in this repository. This database can be utilized for extracting the useful information regarding the present scenario of bioinformatics inclusive of all research labs funded by government and private bodies of India. In addition to web interface, we also developed mobile to facilitate the scientific community.
First description of a herpesvirus infection in genus Lepus
Authors: Abade dos Santos F.A. , Monteiro M. , Pinto A. , Carvalho C.L. , Peleteiro M.C. , Carvalho P. , Mendonça P. , Carvalho T. , Duarte M.D. 
Published: January 2020

AbstractDuring the necropsies of Iberian hares obtained in 2018/2019, along with signs of the nodular form of myxomatosis, other unexpected external lesions were also observed. Histopathology revealed nuclear inclusion bodies in stromal cells suggesting the additional presence of a nuclear replicating virus.Transmission electron microscopy further demonstrated the presence of herpesvirus particles in the tissues of affected hares.We confirmed the presence of herpesvirus in 13 MYXV-positive hares by PCR and sequencing analysis. Herpesvirus-DNA was also detected in seven healthy hares, suggesting its asymptomatic circulation.Phylogenetic analysis based on DNA polymerase gene, grouped this virus within the gammaherpesviruses, close to herpesviruses from bats and rodents.We propose to name this new virus Leporid gammaherpesvirus 5 (LeHV-5), according to the International Committee on Taxonomy of Viruses standards.The impact of herpesvirus infection on the reproduction and mortality of the Iberian hare is yet unknown but may aggravate the decline of wild populations caused by the recently emerged natural recombinant myxoma virus.
GPCR-mediated clearance of tau in post-synaptic compartments attenuates tau pathologyin vivo
Authors: Schaler Ari W. , Runyan Avery M. , Fowler Stephanie L. , Figueroa Helen Y. , Shioda Seiji , Santa-Maria Ismael , Duff Karen E. , Myeku Natura 
Published: January 2020

AbstractAccumulation of pathological tau in synapses has been identified as an early pathogenic event in Alzheimer’s disease (AD) and correlates strongly with cognitive decline in patients with AD. Tau is a cytosolic, axonal protein. However, in the disease condition, tau accumulates in post-synaptic compartments and pre-synaptic terminals, either due to missorting within neurons, trans-synaptic transfer between neurons or due to failure of clearance systems in synapses. Using a sub-cellular fractionation assay, we show that progressive deposition of seed competent tau occurs predominantly in post-synaptic compartments in a tau transgenic mouse and in AD patient brain, making these neuronal structures particularly vulnerable to tau toxicity. Tau-mediated post-synaptic toxicity could be further exacerbated by impaired proteasome activity which we detected by measuring the levels of polyubiquitin chains that target proteins to proteasomal degradation. To combat the accumulation of tau and proteasome impairment at the subcellular level, we devised a therapeutic strategy of proteasome-mediated clearance of tau restricted to the post-synaptic compartment. Utilizing the pharmacology of GPCRs, we show thatin vivostimulation of the PAC1R receptor by its ligand can propagate intracellular PKA signaling leading to enhanced synaptic proteasome activity and reduced tau in the post-synaptic compartment. Over time, clearance of post-synaptic tau led to reduced tauopathy and cognitive decline in rTg4510 mice. Together, these results highlight a novel therapeutic strategy of targeting GPCRs that propagate cAMP/PKA signaling as a tool to activate proteolysis restricted to synapses to prevent the accumulation of tau in the early stages of AD.
Neural differentiation is moderated by age in scene- but not face-selective cortical regions
Authors: Srokova Sabina , Hill Paul F. , Koen Joshua D. , King Danielle R. , Rugg Michael D. 
Published: January 2020

AbstractThe aging brain is characterized by neural dedifferentiation – an apparent decrease in the functional selectivity of category-selective cortical regions. Age-related reductions in neural differentiation have been proposed to play a causal role in cognitive aging. Recent findings suggest, however, that age-related dedifferentiation is not equally evident for all stimulus categories and, additionally, that the relationship between neural differentiation and cognitive performance is not moderated by age. In light of these findings, in the present experiment younger and older human adults (males and females) underwent fMRI as they studied words paired with images of scenes or faces prior to a subsequent memory task. Neural selectivity was measured in two scene-selective (parahippocampal place area and retrosplenial cortex) and two face-selective (fusiform and occipital face areas) regions of interest using both a univariate differentiation index and multivoxel pattern similarity analysis. Both methods provided highly convergent results which revealed evidence of age-related reductions in neural dedifferentiation in scene-selective but not face-selective cortical regions. Additionally, neural differentiation in the parahippocampal place area demonstrated a positive, age-invariant relationship with subsequent source memory performance (recall of the image category paired with each recognized test word). These findings extend prior findings suggesting that age-related neural dedifferentiation is not a ubiquitous phenomenon, and that the specificity of neural responses to scenes is predictive subsequent memory performance independently of age.Significance StatementIncreasing age is associated with reduced neural specificity in cortical regions that are selectively responsive to a given perceptual stimulus category (age-related neural dedifferentiation), a phenomenon which has been proposed to contribute to cognitive aging. Recent findings reveal that age-related neural dedifferentiation is not present for all types of visual stimulus categories, and the factors which determine when the phenomenon arises remain unclear. Here, we demonstrate that scene- but not face-selective cortical regions exhibit age-related neural dedifferentiation during an attentionally-demanding task. Additionally, we report that higher neural selectivity in the scene-selective ‘parahippocampal place area’ is associated with better memory performance after controlling for variance associated with age group, adding to evidence that neural differentiation impacts cognition across the adult lifespan.
A novel strategy for single-cell metabolic analysis highlights dynamic changes in immune subpopulations
Authors: Ahl Patricia J. , Hopkins Richard A. , Xiang Wen Wei , Au Bijin , Kaliaperumal Nivashini , Fairhurst Anna-Marie , Connolly John E. 
Published: January 2020

SummaryA complex interaction of anabolic and catabolic metabolism underpins the ability of leukocytes to mount an immune response. Their capacity to respond and adapt to changing environments by metabolic reprogramming is crucial to their effector function. However, current methods lack the ability to interrogate this network of metabolic pathways at the single cell level within a heterogeneous population. Here we present Met-Flow, a novel flow cytometry-based method that captures the metabolic state of immune cells by targeting key proteins and rate-limiting enzymes across multiple pathways. We demonstrate the ability to simultaneously measure divergent metabolic profiles and dynamic remodeling in human peripheral blood mononuclear cells. Using Met-Flow, we discovered that glucose restriction and metabolic remodeling drive the expansion of an inflammatory central memory T cell subset. This method captures the complex metabolic state of any cell as it relates to its phenotype and function, leading to a greater understanding of the role of metabolic heterogeneity in immune responses.
Taking a landscape approach to conservation goals: designing multi-objective landscapes
Authors: Renwick Anna R. , Chauvenet Alienor L.M. , Possingham Hugh P. , Adams Vanessa M. , McGowan Jennifer , Gagic Vesna , Schellhorn Nancy A. 
Published: January 2020

AbstractDesigning landscapes to accommodate both humans and nature poses huge challenges, but is increasingly recognised as an essential component of conservation and land management. The land-sparing land-sharing framework has been proposed as a tool to address this challenge. However, it has been largely criticised for its simplicity. We provide a new conceptual framework amenable to the application of structured decision-making that moves beyond the dichotomy of land-sparing or land-sharing. Using this new framework, we present a general system model that can be used to make land management decisions for the conservation of species, ecosystem services and production land at different spatial scales. The model can be parameterised for specific systems using information about: the current state of the landscape, the rates of change between landscape states, and the cost and effectiveness of taking actions. To demonstrate the utility of the model we apply it to three different landscape types. Across our three case studies, we show that investment into one of three management actions (varying degrees of management and restoration) can move the system towards more biodiversity or more managed land depending on the objectives of the land manager. We show that the dynamic and flexible nature of the landscape is important to take into account rather than a static snapshot in time. Rather than focusing on establishing the perfect landscape with a set proportion dedicated to production and to biodiversity conservation, we argue that a more useful approach is to establish incremental movements towards a landscape that meets the goals of multiple objectives. Our framework can be used to illustrate to decision makers the costs and trade-offs of different actions and help them determine land management policy.
Isolation and long-term expansion of a single adult human renal epithelial cell with efficient kidney regeneration capacity
Authors: Wang Yujia , Zhao Zixian , Ma Qiwang , Nie Hao , Sun Yufen , Feng Xiaobei , Zhang Ting , Ma Yu , Nie Jing , Zuo Wei 
Published: January 2020

ABSTRACTA wide spectrum of lethal kidney diseases involves the irreversible destruction of the tubular structures which leads to loss of renal function. A reliable technological platform to culture and transplant adult human-derived cells with nephrogenic potential offers great hope to facilitate human kidney regeneration. Here, we show that in an appropriate feeder cell-based culture system, it is feasible to isolate and long-term expand the progenitor-like SOX9+ renal epithelial cells (SOX9+ RECs) from adult mammalians. Single cell-derived SOX9+ REC lines can be established from human needle biopsy or urine samples with molecular homogeneity and genomic stability maintained during culture. Such cells grown in 3D culture system could self-organize into renal organoids composed of proximal tubular, Loop of Henle (LOH) and distal tubular cells as illustrated by single cell transcriptomic analysis. Once being transplanted into the physically injured mouse kidney, the expanded single human SOX9+ RECs incorporated into the damaged area and demonstrated capacity of regenerating functional tubules in vivo. Altogether, the ability to extensively propagate human SOX9+ REC in culture whilst concomitantly maintaining their intrinsic lineage differentiation commitment suggests their future application in regenerative medicine.
Linker histone H1 determines cell stiffness and differentiation
Authors: Kijima Marie , Yamagishi Hiroyuki , Kawano Riho , Konishi Tomoki , Okumura Takuya , Hayase Masanori , Mizuta Ryushin 
Published: January 2020

ABSTRACTThe flexibility or stiffness, one mechanical property of cells, is a promising label-free biomarker for underlying cytoskeletal or nuclear changes associated with various disease processes and changes in cell state. However, the molecular changes responsible for the whole-cell mechanical stiffness remain to be clarified. Recently, it was shown that the deterministic lateral displacement (DLD) microfluidic device, originally developed for size fractionation of some particles, might be applied to distinguish cells according to their stiffness. In this experiment, using the DLD device and various cell lines differentially expressing histone H1, a positively-charged protein localized in the linker region of chromatin, we found linear relationships between histone H1 quantity and cell stiffness. We also found that the histone H1 quantity affected cell size and even cell differentiation.
Alteration of fecal microbial compositions and bacterial taxa in female osteoporotic patients
Authors: Zhu Cuifeng , Liu Jianguo , Pan Yong , Fan Ye , Jin Leigang , Liu Yan , Zhang Zhentian , Gan Yu , Tang Wei , Li Jinhuan , Xiong Zhuang , Xu Genming , Lin Xiuping , Zhang Yuan , Cai JinChuan , Yang Muxiu , Zhang Leijun , Xiao Liehui , Pan Yi , Wang Kejian , Xu Aimin 
Published: January 2020

AbstractBackgroundGut microbiota, mainly characterized by fecal bacterial compositions, affects human immune system and pathophysiological development. Our aim was to measure the quantitative differences of fecal bacterial compositions between osteoporotic patients and healthy subjects, and to identify novel bacterial taxa that speculate the incidence of osteoporosis in female.MethodWe recruited 104 female subjects, including 45 osteoporotic individuals and 59 healthy control. Fecal samples were collected for further analysis by 16S rRNA quantitative arrays and bioinformatics analysis.ResultsAnalyses of α- and β-diversity demonstrated that the diversity and composition of fecal bacterial compositions were both significant different in osteoporosis group, as compared with healthy group. Multiple bacterial genera were significantly increased (e.g., Roseburia and Bacteroides) or decreased (e.g., Streptococcus and Dorea) in the osteoporotic cases. Furthermore, the osteoporosiscould be efficiently determined by the random forest model based on differential taxa (area under ROC curve = 0.93).ConclusionThere were obvious different fecal microbial characteristics between female osteoporosis and healthy subjects. These findings provided evidence for understanding the host-gut microbiota interplay in female osteoporosis, and supported clinical applications of gut microbiota analysis for female osteoporosis diagnosis
Novel ionophores active against La Crosse virus identified through rapid antiviral screening
Authors: Sandler Zachary J. , Vu Michelle N. , Menachery Vineet D. , Mounce Bryan C. 
Published: January 2020

AbstractBunyaviruses are significant human pathogens, causing diseases ranging from hemorrhagic fevers to encephalitis. Among these viruses, La Crosse virus (LACV), a member of the California serogroup, circulates in the eastern and midwestern United States. While LACV infection is often asymptomatic, dozens of cases of encephalitis are reported yearly. Unfortunately, no antivirals have been approved to treat LACV infection. Here, we developed a method to rapidly test potential antivirals against LACV infection. From this screen, we identified several potential antiviral molecules, including known antivirals. Additionally, we identified many novel antivirals that exhibited antiviral activity without affecting cellular viability. Valinomycin, a potassium ionophore, was among our top targets. We found that valinomycin exhibited potent anti-LACV activity in multiple cell types in a dose-dependent manner. Valinomycin did not affect particle stability or infectivity, suggesting that it may preclude virus replication by altering cellular potassium ions, a known determinant of LACV entry. We extended these results to other ionophores and found that the antiviral activity of valinomycin extended to other viral families including bunyaviruses (Rift Valley fever virus, Keystone virus), enteroviruses (Coxsackievirus, rhinovirus), flavirivuses (Zika), and coronaviruses (229E and MERS-CoV). In all viral infections, we observed significant reductions in virus titer in valinomycin-treated cells. In sum, we demonstrate the importance of potassium ions to virus infection, suggesting a potential therapeutic target to disrupt virus replication.ImportanceNo antivirals are approved for the treatment of bunyavirus infection. The ability to rapidly screen compounds and identify novel antivirals is one means to accelerate drug discovery for viruses with no approved treatments. We used this approach to screen hundreds of compounds against La Crosse virus, an emerging bunyavirus that causes significant disease, including encephalitis. We identified several known and previously unidentified antivirals. We focused on a potassium ionophore, valinomycin, due to its promisingin vitroantiviral activity. We demonstrate that valinomycin, as well as a selection of other ionophores, exhibits activity against La Crosse virus as well as several other distantly related bunyaviruses. We finally observe that valinomycin has activity against a wide array of human viral pathogens, suggesting that disrupting potassium ion homeostasis with valinomycin may be a potent host pathway to target to quell virus infection.