bluuebunny/crossref_metadata_2025_split huggingface dataset:
Broadcast Meteorologists’ Views on Climate Change: A State-of-the-Community Review
Authors: Perkins David R. , Timm Kristin , Myers Teresa , Maibach Edward 
Published: January 2020

AbstractBroadcast meteorologists—highly skilled professionals who work at the intersection between climate scientists and the public—have considerable opportunity to educate their viewers about the local impacts of global climate change. Prior research has shown that, within the broadcast meteorology community, views of climate change have evolved rapidly over the past decade. Here, using data from three census surveys of U.S. broadcast meteorologists conducted annually between 2015 and 2017, is a comprehensive analysis of broadcast meteorologists’ views about climate change. Specifically, this research describes weathercasters’ beliefs about climate change and certainty in those beliefs, perceived causes of climate change, perceived scientific consensus and interest in learning more about climate change, belief that climate change is occurring (and the certainty of that belief), belief that climate change is human caused, perceptions of any local impacts of climate change, and perceptions of the solvability of climate change. Today’s weathercaster community appears to be sharing the same viewpoints and outlooks as most climate scientists—in particular, that climate change is already affecting the United States and that present-day trends are largely a result of human activity.
Metachronous Sudden Sensorineural Hearing Loss: Patient Characteristics and Treatment Outcomes
Authors: Cooper Timothy , Dixit Ronak , Hobson Candace E. , Hirsch Barry E. , McCall Andrew A. 
Published: January 2020

ObjectivesIdiopathic sudden sensorineural hearing loss (ISSNHL) is a distressing condition that can significantly affect quality of life. Unilateral ISSNHL, occurring first in 1 ear and then the contralateral ear at a separate and discrete time, is a rare presentation that we refer to as metachronous ISSNHL. Our objective was to characterize the presentation of metachronous ISSNHL and report on management and hearing outcomes.Study DesignRetrospective case series.SettingOtology clinic at an academic tertiary referral center.Subjects and MethodsPatients ≥18 years old presenting with metachronous ISSNHL between April 2008 to November 2017 were identified through review of the clinic electronic medical record. Metachronous ISSNHL was defined as unilateral ISSNHL occurring in temporally discrete episodes (>6 months apart) affecting both ears. Patients with identifiable causes for sudden hearing loss were excluded. Patient demographics, comorbidities, management, and audiologic outcomes were recorded.ResultsEleven patients with metachronous ISSNHL were identified out of 558 patients with ISSNHL. In patients with metachronous ISSNHL, the mean ± standard deviation age at the time of ISSNHL in the second ear was 58.6 ± 15.2 years (range, 31‐77 years). The mean interval between episodes was 9.6 ± 7.5 years (range, 1‐22 years). Patients were treated with systemic and intratympanic steroids with variable hearing recovery; 5 patients with resultant bilateral severe to profound hearing loss underwent successful cochlear implantation.ConclusionMetachronous ISSNHL is uncommon. Treatment is similar to ISSNHL, and cochlear implantation can successfully restore hearing in individuals who do not experience recovery.
Seasonal variability in metal and metalloid burdens of mussels: using data from the German Environmental Specimen Bank to evaluate implications for long-term mussel monitoring programs
Authors: Knopf Burkhard , Fliedner Annette , Radermacher Georg , Rüdel Heinz , Paulus Martin , Pirntke Ulrike , Koschorreck Jan 
Published: January 2020

Abstract
Background
Metal and metalloid concentrations in mussels can vary between seasons. In biota monitoring, the sampling time is therefore an important issue. Within the German Environmental Specimen Bank (ESB) program blue mussels (Mytilus edulis Complex) are sampled regularly since the 1980s. The samples are collected in two-monthly intervals at two North Sea sites and in 6-month intervals at one Baltic Sea site. All samples from one site and year are combined to annual composite samples and archived as sub-samples under cryogenic conditions. In order to investigate a possible reduction of the number of annual sampling intervals while maintaining comparability with the long-term composite sample data, the seasonal variability of metals/metalloids was analyzed based on the half-yearly and bimonthly samples of 2013, 2015 and 2017.

Results
In mussels from the North Sea site Eckwarderhoerne seasonality of metals/metalloids was comparable in all 3 years (arsenic being the only exception). At the North Sea site Koenigshafen seasonality of cobalt, nickel, cadmium, copper, lead, and arsenic was comparable in 2013 and 2015 but not in 2017, while selenium showed the same seasonality in all 3 years. Within 1 year, concentrations of metals and metalloids can vary by the same order of magnitude as observed between annual composite samples of different years making it impossible to select just one representative sampling time point per year that would provide the same information as the respective annual composite sample.

Conclusions
The findings highlight the importance of carefully selecting the sampling time point when using mussels in biota monitoring. For the German ESB program it is recommended to continue with the current sampling strategy and analyze annual composite samples in order to maintain comparability with the long-term data series, which are a special feature of the ESB.
The utility of intravenous ketamine for the management of intraoperative penile erection: a retrospective single-center analysis of endourological surgeries over a 4-year
Authors: Eroğlu Aşkın , Tuncalı Bahattin , Ekin Rahmi Gökhan 
Published: January 2020

Abstract
Background
To assess the prevalence of intraoperative penile erection in our endourology practice and the utility of intravenous ketamine in the management of the condition.

Methods
Of 402 endoscopic urological procedures performed in our clinic over a 4-year (2015–2019) period, a total of 9 cases with intraoperative penile erection impeding instrumentation during endourological surgery were included. Data on patient age, weight, height, American Society of Anesthesiologists (ASA) physical status classification system scores, type and duration of surgery, type and level of anesthesia, onset of erection, treatment characteristics and treatment outcome were recorded for each patient.

Results
The mean (SD) age was 68.3 years (range, 66.0–77.0 years). ASA physical status category I and II were noted in 55.6 and 44.4% of patients, respectively. All cases received spinal anesthesia (n = 9) at T8–10 dermatome levels, for TURP in 7 (77.8%) cases and for TURBT in 2 (22.2%) cases. The onset of penile erection was post-urethroscope in 7 (77.8%) cases. The average total ketamine dose was 34.3 mg (range, 18.0–75.0 mg). The average duration of the operation was 91.7 min (range, 40.0–140.0 min). Ketamine treatment resulted in resolved erection with delayed procedure in 7 (77.8%) cases, while conversion to general anesthesia was required in 2 (22.5%) cases.

Conclusions
In conclusion, the prevalence of intraoperative penile erection during spinal anesthesia for endourological surgery was 2.2% in our experience. These findings demonstrated that intravenous injection of ketamine is an effective and safe method for immediate resolution of intraoperative penile erection with a high success rate.
The round goby genome provides insights into mechanisms that may facilitate biological invasions
Authors: Adrian-Kalchhauser Irene , Blomberg Anders , Larsson Tomas , Musilova Zuzana , Peart Claire R. , Pippel Martin , Solbakken Monica Hongroe , Suurväli Jaanus , Walser Jean-Claude , Wilson Joanna Yvonne , Alm Rosenblad Magnus , Burguera Demian , Gutnik Silvia , Michiels Nico , Töpel Mats , Pankov Kirill , Schloissnig Siegfried , Winkler Sylke 
Published: January 2020

Abstract
Background
The invasive benthic round goby (Neogobius melanostomus) is the most successful temperate invasive fish and has spread in aquatic ecosystems on both sides of the Atlantic. Invasive species constitute powerful in situ experimental systems to study fast adaptation and directional selection on short ecological timescales and present promising case studies to understand factors involved the impressive ability of some species to colonize novel environments. We seize the unique opportunity presented by the round goby invasion to study genomic substrates potentially involved in colonization success.

Results
We report a highly contiguous long-read-based genome and analyze gene families that we hypothesize to relate to the ability of these fish to deal with novel environments. The analyses provide novel insights from the large evolutionary scale to the small species-specific scale. We describe expansions in specific cytochrome P450 enzymes, a remarkably diverse innate immune system, an ancient duplication in red light vision accompanied by red skin fluorescence, evolutionary patterns of epigenetic regulators, and the presence of osmoregulatory genes that may have contributed to the round goby’s capacity to invade cold and salty waters. A recurring theme across all analyzed gene families is gene expansions.

Conclusions
The expanded innate immune system of round goby may potentially contribute to its ability to colonize novel areas. Since other gene families also feature copy number expansions in the round goby, and since other Gobiidae also feature fascinating environmental adaptations and are excellent colonizers, further long-read genome approaches across the goby family may reveal whether gene copy number expansions are more generally related to the ability to conquer new habitats in Gobiidae or in fish.
Decreased expression of a phagocytic receptor Siglec-1 on alveolar macrophages in chronic obstructive pulmonary disease
Authors: Tanno Atsushi , Fujino Naoya , Yamada Mitsuhiro , Sugiura Hisatoshi , Hirano Taizou , Tanaka Rie , Sano Hirohito , Suzuki Satoshi , Okada Yoshinori , Ichinose Masakazu 
Published: January 2020

Abstract
Background
Alveolar macrophages are professional phagocytes that remove microbial pathogens inhaled into the lung. The phagocytic ability is compromised in chronic obstructive pulmonary disease (COPD). However, the molecular mechanisms underlying this defect in phagocytosis are not clearly defined.

Materials and methods
Cell suspensions were collected from lung tissues of patients undergoing lung resection. Alveolar macrophages were detected as FSChi/ SSChi/CD45+/CD206+ cells in the isolated cell suspension by flow-cytometry. The cell surface expression of plasma membrane-bound phagocytic receptors (Fcγ receptor I (FcγRI), a complement receptor CD11b, macrophage scavenger receptor-1 (MSR-1), CD36 and Siglec-1) was determined on the alveolar macrophages. Correlations between the expression levels of the phagocytic receptors and disease severity were analysed. Phagocytosis of fluorescence-tagged bacteria by human alveolar macrophages was evaluated.

Results
The flow-cytometry analyses revealed that FcγRI, CD11b, MSR-1 and Siglec-1, but not CD36, were expressed on human alveolar macrophages. Among these receptors, Siglec-1 expression was significantly decreased on alveolar macrophages in COPD ex-smokers (n = 11), compared to control never-smokers (n = 11) or control ex-smokers (n = 9). The Siglec-1 expression on alveolar macrophages was significantly correlated with lung function (forced expiratory volume in 1 s) and with the severity of emphysema. Treatment of human alveolar macrophages with an anti-Siglec1 blocking antibody decreased phagocytosis of non-typeable Haemophilus influenzae (NTHi).

Conclusion
Our findings demonstrated reduced expression of Siglec-1 on alveolar macrophages in COPD, which is involved in engulfment of NTHi.
How does the onset of incontinence affect satisfaction with life among older women and men? Findings from a nationally representative longitudinal study (German Ageing Survey)
Authors: Buczak-Stec Elżbieta , König Hans-Helmut , Hajek André 
Published: January 2020

Abstract
Background
There is a large body of evidence showing that incontinence is associated with decreased health-related quality of life (HRQoL). Moreover, while a few cross-sectional studies have shown that incontinence is associated with decreased life satisfaction, there is a lack of studies regarding whether the onset of incontinence influences life satisfaction of affected individuals longitudinally. Thus, the objective of this study is: (i) to investigate the impact of incontinence on life satisfaction and (ii) whether this effect differed between women and men using a large population-based sample longitudinally.

Methods
Longitudinal data from 2008 to 2014 were retrieved from a nationally representative sample (9869 observations in regression analysis) of community-dwelling individuals aged 40 years and over (German Ageing Survey, DEAS). Physician-diagnosed incontinence was reported by respondents. Life satisfaction was quantified using the well-established Satisfaction with Life Scale. Linear fixed-effects regressions were used.

Results
After adjusting for potential confounders (e.g., self-rated health or depression), regressions revealed that the onset of incontinence was associated with a decline in life satisfaction in men (β = −.25, p < .01), but not in the total sample and in women. These differences were significant (p < .05). In a further sensitivity analysis, individuals with cancer were excluded. However, in terms of significance and effect size, the impact of incontinence on life satisfaction in men remained almost the same in both models.

Conclusions
The onset of incontinence markedly reduces life satisfaction among men aged 40 and over. Interventional strategies to postpone incontinence may assist in maintaining life satisfaction in men.
Task shifting for point-of-care early infant diagnosis: a comparison of the quality of testing between nurses and laboratory personnel in Zimbabwe
Authors: Simmonds Francis M. , Cohn Jennifer E. , Mafaune Haurovi W. , Nyamundaya Tichaona H. , Mahomva Agnes , Chadambuka Addmore 
Published: January 2020

Abstract
Background
To decentralize point-of-care early infant diagnosis (POC EID), task shifting to cadres such as nurses is important. However, this should not compromise quality of testing through generating high rates of internal quality control (IQC) failures and long result turnaround times. We used data from a POC EID project in Zimbabwe to compare IQC rates and result return to caregivers for samples run on a POC EID technology (Alere q HIV 1/2 Detect) between nurses and laboratory-trained personnel to assess effects of task shifting on quality of testing.

Methods
This cross-sectional retrospective study used data from all 46 sites (10 hub and 36 spoke sites in Zimbabwe that piloted POC EID for routine clinical use from December 2016 to June 2017). IQC failure rates were downloaded from each POC EID platform and exported to excel to analyze IQC failure rates by type of operator. Turnaround time (TAT) from sample collection to issuing of results to caregiver was extracted from the EID test request form and uploaded into a project specific Excel-based database for analysis.

Results
A total of 1847 tests were conducted by 45 testers (12 laboratory-trained and 33 non-laboratory-trained personnel), including 165 errors. There were no significant differences in IQC failure rates between non-laboratory testers (137 [9.2%] of 14830 tests) and specialized laboratory-trained (28 [7.7%] of 364 tests; p = 0.354). Over time, IQC failure rates for both non-laboratory (χ2 = 18.5, p < 0.000) and specialized laboratory-trained testers (χ2 = 8.7, p < 0.003) decreased significantly. There were similar proportions of clients who were issued with results between samples processed by non-laboratory testers (1283 [98.9%] of 1297 tests) and samples processed by specialized laboratory-trained testers (315 [98.7%] of 319 tests; p = 0.790). The overall median turnaround time from sample collection to receipt of results by caregiver for samples run by laboratory-specialized testers was not statistically different from samples run by non-laboratory-specialized testers (1 day [IQR 0–3] versus 0 days [IQR 0–2]; p = 0.583).

Conclusions
Similar IQC failure rates and TATs between non-laboratory and specialized laboratory-trained operators suggest that non-specialized laboratory-trained personnel can perform POC EID equally well as specialized laboratory personnel.
Biologically effective dose (BED) escalation of stereotactic body radiotherapy (SBRT) for hepatocellular carcinoma patients (≤5 cm) with CyberKnife: protocol of study
Authors: Sun Jing , Zhang Aimin , Li Wengang , Wang Quan , Li Dong , Zhang Dan , Duan Xuezhang 
Published: January 2020

Abstract
Background
There is a lack of data on the biologically effective dose and the efficacy of stereotactic body radiotherapy in hepatocellular carcinoma patients, and this study was conducted to explore the relation between BED and efficacy.

Methods
This study is designed as a mono-center study. The participants are randomized into three group, and received the following recommended schedule: 49Gy/7f, 54Gy/6f and 55Gy/5f with BED10 in correspondence to 83.3Gy, 102.6Gy and 115.5Gy. The primary outcome measures are to calculate local control rates (LC), overall survival rates (OS) and progression-free survival rates (PFS). The secondary outcome measures are to observe radiation-induced liver injury (RILD) rates, Child-Pugh score and indocyanine green retention rate at 15 min (ICG-R15) value before and after CK-SBRT. Moreover, gastrointestinal toxicities are also observed.

Discussion
There is no uniform standard for CK-SBRT dose schedule of hepatocellular carcinoma. We propose to conduct a study determining the optimal CK-SBRT schedule of hepatocellular carcinoma patients (≤5 cm). The trial protocol has been approved by the Institutional Review Board of 302 Hospital of PLA (People’s Liberation Army). The Ethics number is 2017111D.

Trail registration
Clinical trails number: NCT03295500. Date of registration: November, 2017.
Clinical evaluation of ultrasonic subgingival debridement versus ultrasonic subgingival scaling combined with manual root planing in the treatment of periodontitis: study protocol for a randomized controlled trial
Authors: Yan Yue , Zhan Yalin , Wang Xian’e , Hou Jianxia 
Published: January 2020

Abstract
Background
Periodontal diseases are regarded as the most common diseases of mankind. The prevalence rate of periodontal disease assumes a clear growth tendency, increasing by 57.3% from 1990 to 2010. Thereby, effective periodontal therapy is still a long-term task and a difficult problem. The goals of periodontal therapy are to eliminate the infectious and inflammatory processes of periodontal diseases. Root planing, in order to eliminate the “infected cementum,” has been an important step in the treatment of periodontitis since the 1970s. However, along with the understanding of the effects of endotoxin on the root surface, the necessity of manual root planing has been gradually queried. Ultrasonic instruments, which are more recent innovations, would not remove the cementum excessively, and are also more time-saving and labor-saving compared to using hand instruments. Hence, an increasing number of dentists prefer to do scaling with ultrasonic instruments only. However, the necessity of root planing remains emphasized in the international mainstream views of periodontal mechanical treatment. Therefore, this study is devoted to compare the clinical effect of ultrasonic subgingival debridement and ultrasonic subgingival scaling combined with manual root planing, which takes the implementation of root planing as the only variable and is more in line with the current clinical situation, thus hoping to provide some valuable reference to dentists.

Methods/design
Forty adult patients who fit the inclusion criteria are being recruited from the Peking University Hospital of Stomatology (Beijing, China). By means of randomization tables, one quadrant of the upper and lower teeth is the test group and the other is the control group. Test group: ultrasonic subgingival scaling combined with manual root planing. Control group: ultrasonic subgingival debridement. In a 24-week follow-up period, plaque index, probing depth, clinical attachment loss, bleeding index, furcation involvement, mobility, and patient-reported outcome (Visual Analog Scale for pain and sensitivity) will be observed and documented.

Discussion
This study evaluates the effectiveness of ultrasonic subgingival scaling combined with manual root planing and ultrasonic subgingival debridement alone in the nonsurgical treatment of periodontitis with a split-mouth design after 1, 3 and 6 months. The result of the trial should potentially contribute to an advanced treatment strategy for periodontitis with an ideal clinical outcome.

Trial registration
International Clinical Trials Registry Platform (ICTRP), ID: ChiCTR1800017122. Registered on 12 July 2018.
Interactions between the gut microbiome and host gene regulation in cystic fibrosis
Authors: Dayama Gargi , Priya Sambhawa , Niccum David E. , Khoruts Alexander , Blekhman Ran 
Published: January 2020

AbstractBackgroundCystic fibrosis is the most common autosomal recessive genetic disease in Caucasians. It is caused by mutations in theCFTRgene, leading to poor hydration of mucus and impairment of the respiratory, digestive, and reproductive organ functions. Advancements in medical care have led to markedly increased longevity of patients with cystic fibrosis, but new complications have emerged, such as early onset of colorectal cancer. Although the pathogenesis of colorectal cancer in cystic fibrosis remains unclear, altered host-microbe interactions might play a critical role. To investigate this, we characterized changes in the microbiome and host gene expression in the colonic mucosa of cystic fibrosis patients relative to healthy controls, and identified host gene-microbiome interactions in the colon of cystic fibrosis patients.MethodsWe performed RNA-seq on colonic mucosa samples from cystic fibrosis patients and healthy controls to determine differentially expressed host genes. We also performed 16S rRNA sequencing to characterize the colonic mucosal microbiome and identify gut microbes that are differentially abundant between patients and healthy controls. Lastly, we modeled associations between relative abundances of specific bacterial taxa in the gut mucosa and host gene expression.ResultsWe find that 1543 genes, includingCFTR, show differential expression in the colon of cystic fibrosis patients compared to healthy controls. These genes are enriched with functions related to gastrointestinal and colorectal cancer, such as metastasis of colorectal cancer, tumor suppression, p53, and mTOR signaling pathways. In addition, patients with cystic fibrosis show decreased gut microbial diversity, decreased abundance of butyrate producing bacteria, such as Ruminococcaceae andButyricimonas, and increased abundance of other taxa, such as Actinobacteria andClostridium. An integrative analysis identified colorectal cancer-related genes, includingLCN2andDUOX2, for which gene expression is correlated with the abundance of colorectal cancer-associated bacteria, such as Ruminococcaceae andVeillonella.ConclusionsIn addition to characterizing host gene expression and mucosal microbiome in cystic fibrosis patients, our study explored the potential role of host-microbe interactions in the etiology of colorectal cancer in cystic fibrosis. Our results provide biomarkers that may potentially serve as targets for stratifying risk of colorectal cancer in patients with cystic fibrosis.