bluuebunny/crossref_metadata_2025_split huggingface dataset:
Changes in monocyte subsets are associated with clinical outcomes in severe malarial anaemia and cerebral malaria
Authors: Royo Jade , Rahabi Mouna , Kamaliddin Claire , Ezinmegnon Sem , Olagnier David , Authier Hélène , Massougbodji Achille , Alao Jules , Ladipo Yélé , Deloron Philippe , Bertin Gwladys , Pipy Bernard , Coste Agnès , Aubouy Agnès 
Published: November 2019

AbstractMonocytes are plastic heterogeneous immune cells involved in host-parasite interactions critical for malaria pathogenesis. Human monocytes have been subdivided into three populations based on surface expression of CD14 and CD16. We hypothesised that proportions and phenotypes of circulating monocyte subsets can be markers of severity or fatality in children with malaria. To address this question, we compared monocytes sampled in children with uncomplicated malaria, severe malarial anaemia, or cerebral malaria. Flow cytometry was used to distinguish and phenotype monocyte subsets through CD14, CD16, CD36 and TLR2 expression. Data were first analysed by univariate analysis to evaluate their link to severity and death. Second, multinomial logistic regression was used to measure the specific effect of monocyte proportions and phenotypes on severity and death, after adjustments for other variables unrelated to monocytes. Multivariate analysis demonstrated that decreased percentages of non-classical monocytes were associated with death, suggesting that this monocyte subset has a role in resolving malaria. Using univariate analysis, we also showed that the role of non-classical monocytes involves a mostly anti-inflammatory profile and the expression of CD16. Further studies are needed to decipher the functions of this sub-population during severe malaria episodes, and understand the underlying mechanisms.
Space division multiplexing in standard multi-mode optical fibers based on speckle pattern classification
Authors: Pauwels Jaël , Van der Sande Guy , Verschaffelt Guy 
Published: November 2019

AbstractIn optical communications the transmission bandwidth of single mode optical fibers is almost fully exploited. To further increase the capacity of a telecommunication link, multiplexing techniques can be applied across 5 physical dimensions: amplitude, quadrature, polarization, frequency and space, with all but the latter being nearly exhausted. We experimentally demonstrate the feasibility of an original space division multiplexing technique based on the classification of speckle patterns measured at the fiber’s output. By coupling multiple optical signals into a standard multimode optical fiber, speckle patterns arise at the fiber’s end facet. This is due to quasi-random interference between the excited modes of propagation. We show how these patterns depend on the parameters of the optical signal beams and the fiber length. Classification of the speckle patterns allows the detection of the independent signals: we can detect the state (i.e. on or off  ) of different beams that are multiplexed in the fiber. Our results show that the proposed space division multiplexing on standard multimode fibers is robust to mode-mixing and polarization scrambling effects.
3D-Printing and Machine Learning Control of Soft Ionic Polymer-Metal Composite Actuators
Authors: Carrico James D. , Hermans Tucker , Kim Kwang J. , Leang Kam K. 
Published: November 2019

AbstractThis paper presents a new manufacturing and control paradigm for developing soft ionic polymer-metal composite (IPMC) actuators for soft robotics applications. First, an additive manufacturing method that exploits the fused-filament (3D printing) process is described to overcome challenges with existing methods of creating custom-shaped IPMC actuators. By working with ionomeric precursor material, the 3D-printing process enables the creation of 3D monolithic IPMC devices where ultimately integrated sensors and actuators can be achieved. Second, Bayesian optimization is used as a learning-based control approach to help mitigate complex time-varying dynamic effects in 3D-printed actuators. This approach overcomes the challenges with existing methods where complex models or continuous sensor feedback are needed. The manufacturing and control paradigm is applied to create and control the behavior of example actuators, and subsequently the actuator components are combined to create an example modular reconfigurable IPMC soft crawling robot to demonstrate feasibility. Two hypotheses related to the effectiveness of the machine-learning process are tested. Results show enhancement of actuator performance through machine learning, and the proof-of-concepts can be leveraged for continued advancement of more complex IPMC devices. Emerging challenges are also highlighted.
A comparison of thermographic characteristics of the hands and wrists of rheumatoid arthritis patients and healthy controls
Authors: Gatt Alfred , Mercieca Cecilia , Borg Andrew , Grech Andrea , Camilleri Liberato , Gatt Corene , Chockalingam Nachiappan , Formosa Cynthia 
Published: November 2019

AbstractThermal imaging has been applied to detect possible temperature variations in various rheumatic disorders. This study sought to determine whether rheumatoid arthritis (RA) patients without active synovitis in their hands exhibit different baseline thermographic patterns of the fingers and palms when compared to healthy individuals. Data from 31 RA patients were compared to that of 51 healthy controls. The RA patients were recruited upon confirmed absence of synovitis by clinical examination and musculoskeletal ultrasound. Participants underwent medical infrared imaging of the regions of interest (ROIs). Significant differences were found between the mean temperatures of the palm regions (29.37 °C (SD2.2); n = 306) and fingers (27.16 °C (SD3.2); n = 510) of the healthy participants when compared to the palm regions (31.4(SD1.84)°C; n = 186) and fingers (30.22 °C (SD2.4); n = 299) of their RA counterparts (p = 0.001), with the latter group exhibiting higher temperatures in all ROIs. Logistic regression models confirm that both palm and finger temperature increase significantly in RA without active inflammation. These innovative findings provide evidence that baseline thermal data in RA differs significantly from healthy individuals. Thermal imaging may have the potential to become an adjunct assessment method of disease activity in patients with RA.
Natural infection by the protozoan Leptomonas wallacei impacts the morphology, physiology, reproduction, and lifespan of the insect Oncopeltus fasciatus
Authors: Vasconcellos Luiz Ricardo C. , Carvalho Luiz Max F. , Silveira Fernanda A. M. , Gonçalves Inês C. , Coelho Felipe S. , Talyuli Octávio A. C. , Alves e Silva Thiago L. , Bastos Leonardo S. , Sorgine Marcos H. F. , Reis Leonan A. , Dias Felipe A. , Struchiner Claudio J. , Gazos-Lopes Felipe , Lopes Angela H. 
Published: November 2019

AbstractTrypanosomatids are protozoan parasites that infect thousands of globally dispersed hosts, potentially affecting their physiology. Several species of trypanosomatids are commonly found in phytophagous insects. Leptomonas wallacei is a gut-restricted insect trypanosomatid only retrieved from Oncopeltus fasciatus. The insects get infected by coprophagy and transovum transmission of L. wallacei cysts. The main goal of the present study was to investigate the effects of a natural infection by L. wallacei on the hemipteran insect O. fasciatus, by comparing infected and uninfected individuals in a controlled environment. The L. wallacei-infected individuals showed reduced lifespan and morphological alterations. Also, we demonstrated a higher infection burden in females than in males. The infection caused by L. wallacei reduced host reproductive fitness by negatively impacting egg load, oviposition, and eclosion, and promoting an increase in egg reabsorption. Moreover, we associated the egg reabsorption observed in infected females, with a decrease in the intersex gene expression. Finally, we suggest alterations in population dynamics induced by L. wallacei infection using a mathematical model. Collectively, our findings demonstrated that L. wallacei infection negatively affected the physiology of O. fasciatus, which suggests that L. wallacei potentially has a vast ecological impact on host population growth.
Bisphenol-A (BPA) in Foods commonly consumed in Southwest Nigeria and its Human Health Risk
Authors: Adeyi Adebola A. , Babalola Babafemi A. 
Published: November 2019

AbstractBisphenol-A (BPA) is a synthetic chemical ubiquitous in the environment and listed as an endocrine disruptor. It has the tendency of migrating into food stored in materials containing it. This study, therefore, determines the concentrations of BPA in foods commonly consumed in Southwest Nigeria by the adult population and also estimates the risk associated with human exposure. Eight different food categories were selected for this study. Standard QuEChERS protocol was used for sample extraction and analysed using gas chromatography-mass spectrometry (GC-MS). Vegetable oil had the highest BPA concentration (28.4 ng/g). This was followed by aquatic canned fish (26.3 ng/g), canned beef (21.3 ng/g) and crayfish (17.5 ng/g). These concentrations were below the 600 ng/g limit of the European Commission for BPA in foods. Bisphenol-A was not detected in raw beef, chicken, cheese, apple, tomatoes, beans and rice; and chicken eggs. The adult population had an average dietary intake of 30.4 ng/kg bw/day. There is no likely occurrence of harmful health effects of BPA in the selected foods with respect to the current concentrations found therein. However, routine monitoring is recommended to prevent human exposure to BPA.
Chemo-treated 4T1 breast cancer cells radiation response measured by single and multiple cell ionization using infrared laser trap
Authors: Muhammed Endris , Chen Li , Gao Ying , Erenso Daniel 
Published: November 2019

AbstractWe present a study that uses a laser trapping technique for measurement of radiation sensitivity of untreated and chemo-treated cancer cells. We used a human mammary tumor cell line (4T1) treated by an antitumor compound, 2-Dodecyl-6-methoxycyclohexa-2, 5-diene-1,4-dione (DMDD), which was extracted from the root of Averrhoa carambola L. The untreated control group, and both 2-hour and 24-hour treated groups of 4T1 cells were used in this study. The absorbed threshold ionization energy (TIE) and the threshold radiation dose (TRD) were determined using a high-power infrared laser (at 1064 nm) trap by single and multiple cells trapping and ionization. The results were analyzed using descriptive and t-statistics. The relation of the TIE and TRD to the mass of the individual cells were also analyzed for different hours of treatment in comparison with the control group. Both TIE and TRD decrease with increasing treatment periods. However, the TRD decreases with mass regardless of the treatment. Analyses of the TRD for single vs multiple cells ionizations within each group have also consistently showed this same behavior regardless of the treatment. The underlying factors for these observed relations are explained in terms of radiation, hyperthermia, and chemo effects.
Novel miR-29b target regulation patterns are revealed in two different cell lines
Authors: Zhao Wenting , Cheng Lesley , Quek Camelia , Bellingham Shayne A. , Hill Andrew F. 
Published: November 2019

AbstractMicroRNAs (miRNAs) are a class of small non-coding RNAs that regulate gene or protein expression by targeting mRNAs and triggering either translational repression or mRNA degradation. Distinct expression levels of miRNAs, including miR-29b, have been detected in various biological fluids and tissues from a large variety of disease models. However, how miRNAs “react” and function in different cellular environments is still largely unknown. In this study, the regulation patterns of miR-29b between human and mouse cell lines were compared for the first time. CRISPR/Cas9 gene editing was used to stably knockdown miR-29b in human cancer HeLa cells and mouse fibroblast NIH/3T3 cells with minimum off-targets. Genome editing revealed mir-29b-1, other than mir-29b-2, to be the main source of generating mature miR-29b. The editing of miR-29b decreased expression levels of its family members miR-29a/c via changing the tertiary structures of surrounding nucleotides. Comparing transcriptome profiles of human and mouse cell lines, miR-29b displayed common regulation pathways involving distinct downstream targets in macromolecular complex assembly, cell cycle regulation, and Wnt and PI3K-Akt signalling pathways; miR-29b also demonstrated specific functions reflecting cell characteristics, including fibrosis and neuronal regulations in NIH/3T3 cells and tumorigenesis and cellular senescence in HeLa cells.
Proteome characterization of used nesting material and potential protein sources from group housed male mice, Mus musculus
Authors: Barabas Amanda J. , Aryal Uma K. , Gaskill Brianna N. 
Published: November 2019

AbstractLaboratory mice (Mus musculus)communicate a variety of social messages through olfactory cues and it is often speculated that these cues are preserved in nesting material. Based on these speculations, a growing number of husbandry recommendations support preserving used nests at cage cleaning to maintain familiar odors in the new cage. However, the content of used nesting material has never been chemically analyzed. Here we present the first comprehensive proteome profile of used nesting material. Nests from cages of group housed male mice contain a variety of proteins that primarily originate from saliva, plantar sweat, and urine sources. Most notably, a large proportion of proteins found in used nesting material belong to major urinary protein (“MUP”) and odorant binding protein (“OBP”) families. Both protein families send messages about individual identity and bind volatile compounds that further contribute to identity cues. Overall, this data supports current recommendations to preserve used nesting material at cage cleaning to maintain odor familiarity.
Distribution pattern and molecular signature of cholinergic tuft cells in human gastro-intestinal and pancreatic-biliary tract
Authors: Schütz Burkhard , Ruppert Anna-Lena , Strobel Oliver , Lazarus Michael , Urade Yoshihiro , Büchler Markus W. , Weihe Eberhard 
Published: November 2019

AbstractDespite considerable recent insight into the molecular phenotypes and type 2 innate immune functions of tuft cells in rodents, there is sparse knowledge about the region-specific presence and molecular phenotypes of tuft cells in the human digestive tract. Here, we traced cholinergic tuft cells throughout the human alimentary tract with immunohistochemistry and deciphered their region-specific distribution and biomolecule coexistence patterns. While absent from the human stomach, cholinergic tuft cells localized to villi and crypts in the small and large intestines. In the biliary tract, they were present in the epithelium of extra-hepatic peribiliary glands, but not observed in the epithelia of the gall bladder and the common duct of the biliary tract. In the pancreas, solitary cholinergic tuft cells were frequently observed in the epithelia of small and medium-size intra- and inter-lobular ducts, while they were absent from acinar cells and from the main pancreatic duct. Double immunofluorescence revealed co-expression of choline acetyltransferase with structural (cytokeratin 18, villin, advillin) tuft cell markers and eicosanoid signaling (cyclooxygenase 1, hematopoietic prostaglandin D synthase, 5-lipoxygenase activating protein) biomolecules. Our results indicate that region-specific cholinergic signaling of tuft cells plays a role in mucosal immunity in health and disease, especially in infection and cancer.
Ultra-thin ion exchange film on the ceramic supporter for output power improvement of reverse electrodialysis
Authors: Jung Dong Hyeon , Han Eui Don , Kim Byeong Hee , Seo Young Ho 
Published: November 2019

AbstractIn this study, ultra-thin ion exchange film on the ceramic supporter (UTFCS) composed of thin polymer layer and nanoporous ceramic layer with low electrical resistance was developed. The electrical properties and permselectivity of UTFCSs were evaluated and the properties of UTFCSs were compared with other ion exchange membranes. Fabricated UTFCSs were applied to a reverse electrodialysis (RED) system to evaluate the output characteristics and compared with other ion exchange membranes. The power density of RED using UTFCS was 36.6 mW/m2, which was 8% higher than that of a commercial anion exchange membrane. In addition, possibility as power source was experimentally verified by driving LEDs. The proposed UTFCS can be applied not only to RED but also to energy development such as fuel cells and microbial cells.
Mio-Pliocene piracy, relict landscape and drainage reorganization in the Namcha Barwa syntaxis zone of eastern Himalaya
Authors: Jaiswara Nilesh Kumar , Pandey Prabha , Pandey Anand K. 
Published: November 2019

AbstractThe presence of unique elevated low relief relict landscape in the transient Dibang catchment, at the orographic edge of Tibet-Himalaya in the tectonically active Namcha Barwa syntaxial zone, is modelled to understand evolving regional landscape, drainage reorganization and tectonics. This elevated low relief landscape represents a Mio-Pliocene abandoned paleo-channel of the Yarlung river, which was captured by the headward eroding Siang river owing to >600 m base level advantage. The river capture caused isolation of the Dibang river, which evolved as a transient parched catchment since 3–6 Ma after loss of ~17 times drainage area and 4–17 times discharge. The drainage area and discharge gained by the Siang river triggered enormous incision causing aneurysm leading to the accelerated growth of the Tsangpo gorge and affected regional tectonics. This paleo-drainage reorganization is reflected in the Mio-Pliocene sedimentation pattern in the southern Tibet-Himalaya and foreland basins.
Roles of Toll-like receptor 2/4, monoacylglycerol lipase, and cyclooxygenase in social defeat stress-induced prostaglandin E2 synthesis in the brain and their behavioral relevance
Authors: Nie Xiang , Kitaoka Shiho , Shinohara Masakazu , Kakizuka Akira , Narumiya Shuh , Furuyashiki Tomoyuki 
Published: November 2019

AbstractInflammation in the brain and periphery has been associated with stress-related pathology of mental illness. We have shown that prostaglandin (PG) E2, an arachidonic acid-derived lipid mediator, and innate immune receptors Toll-like receptor (TLR) 2/4 are crucial for repeated stress-induced behavioral changes in rodents. However, how the stress induces PGE2 synthesis in the brain and whether TLR2/4 are involved in the PGE2 synthesis remain unknown. Using mice lacking TLR2 and TLR4 in combination, here we show that social defeat stress (SDS) induced the PGE2 synthesis in subcortical, but not cortical, tissues in a TLR2/4-dependent manner. It is known that PGE2 in the brain is mainly derived by monoacylglycerol lipase (MAGL)-mediated conversion of endocannabinoid 2-arachidonoylglycerol to free-arachidonic acid, a substrate for cyclooxygenase (COX) for PGE2 synthesis. We found that TLR2/4 deletion reduced the mRNA expression of MAGL and COX1 in subcortical tissues after repeated SDS. Perturbation of MAGL and COX1 as well as COX2 abolished SDS-induced PGE2 synthesis in subcortical tissues. Furthermore, systemic administration of JZL184, an MAGL inhibitor, abolished repeated SDS-induced social avoidance. These results suggest that SDS induces PGE2 synthesis in subcortical regions of the brain via the MAGL-COX pathway in a TLR2/4-dependent manner, thereby leading to social avoidance.
A New Picture of the Global Impacts of El Nino-Southern Oscillation
Authors: Lin Jialin , Qian Taotao 
Published: November 2019

AbstractThe El Nino-Southern Oscillation (ENSO) is the dominant interannual variability of Earth’s climate system and plays a central role in global climate prediction. Outlooks of ENSO and its impacts often follow a two-tier approach: predicting ENSO sea surface temperature anomaly in tropical Pacific and then predicting its global impacts. However, the current picture of ENSO global impacts widely used by forecasting centers and atmospheric science textbooks came from two earliest surface station datasets complied 30 years ago, and focused on the extreme phases rather than the whole ENSO lifecycle. Here, we demonstrate a new picture of the global impacts of ENSO throughout its whole lifecycle based on the rich latest satellite, in situ and reanalysis datasets. ENSO impacts are much wider than previously thought. There are significant impacts unknown in the previous picture over Europe, Africa, Asia and North America. The so-called “neutral years” are not neutral, but are associated with strong sea surface temperature anomalies in global oceans outside the tropical Pacific, and significant anomalies of land surface air temperature and precipitation over all the continents.
Functional conservation of RecQ helicase BLM between humans and Drosophila melanogaster
Authors: Cox Rebecca L. , Hofley Carolyn M. , Tatapudy Pallavi , Patel Romil K. , Dayani Yaron , Betcher Madison , LaRocque Jeannine R. 
Published: November 2019

AbstractRecQ helicases are a family of proteins involved in maintaining genome integrity with functions in DNA repair, recombination, and replication. The human RecQ helicase family consists of five helicases: BLM, WRN, RECQL, RECQL4, and RECQL5. Inherited mutations in RecQ helicases result in Bloom Syndrome (BLM mutation), Werner Syndrome (WRN mutation), Rothmund-Thomson Syndrome (RECQL4 mutation), and other genetic diseases, including cancer. The RecQ helicase family is evolutionarily conserved, as Drosophila melanogaster have three family members: DmBlm, DmRecQL4, and DmRecQL5 and DmWRNexo, which contains a conserved exonuclease domain. DmBlm has functional similarities to human BLM (hBLM) as mutants demonstrate increased sensitivity to ionizing radiation (IR) and a decrease in DNA double-strand break (DSB) repair. To determine the extent of functional conservation of RecQ helicases, hBLM was expressed in Drosophila using the GAL4 > UASp system to determine if GAL4 > UASp::hBLM can rescue DmBlm mutant sensitivity to IR. hBLM was able to rescue female DmBlm mutant sensitivity to IR, supporting functional conservation. This functional conservation is specific to BLM, as human GAL4 > UASp::RECQL was not able to rescue DmBlm mutant sensitivity to IR. These results demonstrate the conserved role of BLM in maintaining the genome while reinforcing the applicability of using Drosophila as a model system to study Bloom Syndrome.
Enzyme-Assisted Photoinjection of Megadalton Molecules into Intact Plant Cells Using Femtosecond Laser Amplifier
Authors: Rukmana Taufiq Indra , Moran Gabriela , Méallet-Renault Rachel , Ohtani Misato , Demura Taku , Yasukuni Ryohei , Hosokawa Yoichiroh 
Published: November 2019

AbstractFemtosecond laser photoporation has become a popular method to deliver various kinds of molecules such as genes, proteins, and fluorescent dyes into single mammalian cells. However, this method is not easily applied to plant cells because their cell wall and turgor pressure prevent the delivery, especially for larger molecules than the mesh size of the cell wall. This work is the first demonstration of the efficient photoinjection of megadalton molecules into a cytoplasm of an intact single plant cell by employing a femtosecond laser amplifier under moderate enzyme treatment conditions. The intense femtosecond laser pulse effectively formed a pore on the cell wall and membrane of Tobacco BY-2, and 2 MDa dextran molecules were introduced through the pore. Along with the pore formation, induced mechanical tensile stresses on BY-2 cells were considered to increase permeability of the cell membrane and enhance the uptake of large molecules. Moreover, the moderate enzyme treatment partially degraded the cell wall thereby facilitating the increase of the molecular introduction efficiency.