bluuebunny/crossref_metadata_2025_split huggingface dataset:
Establishment and Early Development of Even-Age Shortleaf Pine–Hardwood Mixtures Using Artificially Regenerated Shortleaf Pine and Various Site Preparation and Release Treatments
Authors: Clabo David C , Clatterbuck Wayne K 
Published: December 2019

Abstract
Shortleaf pine (Pinus echinata)–hardwood mixtures were once a common forest type on upland sites throughout the southeastern United States. These forest types have declined throughout much of shortleaf pine’s native range. Information on restoring shortleaf pine–hardwood types when a shortleaf pine seed source is not present is lacking. The objectives of this study were to investigate the effects of four site-preparation and release treatments (control, burn, herbicide, and herbicide and burn) on the development of even-aged mixtures of planted shortleaf pine and natural hardwoods located on the Highland Rim physiographic province of Tennessee, USA. Three years after study establishment, statistical differences among treatments occurred for shortleaf pine survival and basal diameter. Survival was greatest in the herbicide-only treatment. Height and basal diameter growth were greatest in the herbicide and burn treatment. Stem densities were greater in treatments that did not include a herbicide release than those where herbicide was used alone or combined with prescribed burning. The herbicide and burn treatment had the greatest potential after 3 years for developing into a balanced, mixed shortleaf pine–hardwood stand, whereas most treatments had an influx of exotic, invasive species such as Chinese privet (Ligustrum sinense) and callery pear (Pyrus calleryana).
High Transcriptional Error Rates Vary as a Function of Gene Expression Level
Authors: Meer Kendra M , Nelson Paul G , Xiong Kun , Masel Joanna 
Published: December 2019

Abstract
Errors in gene transcription can be costly, and organisms have evolved to prevent their occurrence or mitigate their costs. The simplest interpretation of the drift barrier hypothesis suggests that species with larger population sizes would have lower transcriptional error rates. However, Escherichia coli seems to have a higher transcriptional error rate than species with lower effective population sizes, for example Saccharomyces cerevisiae. This could be explained if selection in E. coli were strong enough to maintain adaptations that mitigate the consequences of transcriptional errors through robustness, on a gene by gene basis, obviating the need for low transcriptional error rates and associated costs of global proofreading. Here, we note that if selection is powerful enough to evolve local robustness, selection should also be powerful enough to locally reduce error rates. We therefore predict that transcriptional error rates will be lower in highly abundant proteins on which selection is strongest. However, we only expect this result when error rates are high enough to significantly impact fitness. As expected, we find such a relationship between expression and transcriptional error rate for non-C→U errors in E. coli (especially G→A), but not in S. cerevisiae. We do not find this pattern for C→U changes in E. coli, presumably because most deamination events occurred during sample preparation, but do for C→U changes in S. cerevisiae, supporting the interpretation that C→U error rates estimated with an improved protocol, and which occur at rates comparable with E. coli non-C→U errors, are biological.
Genome Sequences of 72 Bacterial Strains Isolated from Ectocarpus subulatus: A Resource for Algal Microbiology
Authors: Karimi Elham , Geslain Enora , KleinJan Hetty , Tanguy Gwenn , Legeay Erwan , Corre Erwan , Dittami Simon M 
Published: December 2019

AbstractBrown algae are important primary producers and ecosystem engineers in the ocean, and Ectocarpus has been established as a laboratory model for this lineage. Like most multicellular organisms, Ectocarpus is associated with a community of microorganisms, a partnership frequently referred to as holobiont due to the tight interconnections between the components. Although genomic resources for the algal host are well established, its associated microbiome is poorly characterized from a genomic point of view, limiting the possibilities of using these types of data to study host–microbe interactions. To address this gap in knowledge, we present the annotated draft genome sequences of seventy-two cultivable Ectocarpus-associated bacteria. A screening of gene clusters related to the production of secondary metabolites revealed terpene, bacteriocin, NRPS, PKS-t3, siderophore, PKS-t1, and homoserine lactone clusters to be abundant among the sequenced genomes. These compounds may be used by the bacteria to communicate with the host and other microbes. Moreover, detoxification and provision of vitamin B pathways have been observed in most sequenced genomes, highlighting potential contributions of the bacterial metabolism toward host fitness and survival. The genomes sequenced in this study form a valuable resource for comparative genomic analyses and evolutionary surveys of alga-associated bacteria. They help establish Ectocarpus as a model for brown algal holobionts and will enable the research community to produce testable hypotheses about the molecular interactions within this complex system.
Geodetic evidence for shallow creep along the Quito fault, Ecuador
Authors: Mariniere J , Nocquet J-M , Beauval C , Champenois J , Audin L , Alvarado A , Baize S , Socquet A 
Published: December 2019

SUMMARY
Quito, the capital city of Ecuador hosting ∼2 million inhabitants, lies on the hanging wall of a ∼60-km-long reverse fault offsetting the Inter-Andean Valley in the northern Andes. Such an active fault poses a significant risk, enhanced by the high density of population and overall poor building construction quality. Here, we constrain the present-day strain accumulation associated with the Quito fault with new Global Positioning System (GPS) data and Persistent Scatterer Interferometric Synthetic Aperture Radar (PS-InSAR) analysis. Far field GPS data indicate 3–5 mm yr–1 of horizontal shortening accommodated across the fault system. In the central segment of the fault, both GPS and PS-InSAR results highlight a sharp velocity gradient, which attests for creep taking place along the shallowest portion of the fault. Smoother velocity gradients observed along the other segments indicate that the amount of shallow creep decreases north and south of the central segment. 2-D elastic models using GPS horizontal velocity indicate very shallow (&lt;1 km) locking depth for the central segment, increasing to a few kilometres south and north of it. Including InSAR results in the inversion requires locking to vary both along dip and along strike. 3-D spatially variable locking models show that shallow creep occurs along the central 20-km-long segment. North and south of the central segment, the interseismic coupling is less resolved, and data still allows significant slip deficit to accumulate. Using the interseismic moment deficit buildup resulting from our inversions and the seismicity rate, we estimate recurrence time for magnitude 6.5 + earthquake to be between 200 and 1200 yr. Finally, PS-InSAR time-series identify a 2 cm transient deformation that occurred on a secondary thrust, east of the main Quito fault between 1995 and 1997.
Ultra-low palaeointensities from East European Craton, Ukraine support a globally anomalous palaeomagnetic field in the Ediacaran
Authors: Shcherbakova V V , Bakhmutov V G , Thallner D , Shcherbakov V P , Zhidkov G V , Biggin A J 
Published: December 2019

SUMMARY
The time-averaged geomagnetic field is generally purported to be uniformitarian across Earth history—close to a geocentric axial dipole, with average strength within one order of magnitude of that at present. Nevertheless, recent studies have reported that the field was approximately ten times weaker than present in the mid-Palaeozoic (∼410–360 Ma) and late Ediacaran (∼565 Ma). Here we present the first whole-rock palaeointensity determinations of Ediacaran age outside of Laurentia. These were obtained by the Thellier-Coe, Wilson and microwave methods for basaltic rocks of 560–580 Ma age of the Ediacaran traps, southwestern margin of the East European Craton, Ukraine. All four studied sites showed extremely low instantaneous field values of (3–7) μT with corresponding VDMs of (0.4–1) × 1022 Am2. Summarizing all available data, the Ediacaran field appears to be anomalously characterized by ultra-low dipole moment and ultra-high reversal frequency. According to some geodynamo models, this state could indicate a weak dipole field regime prior to the nucleation of the solid inner core. However, given that ultra-low field intensities have also been detected in the Devonian, and that virtually no palaeointensity data exist for the intervening ∼150 Ma, the date of inner core nucleation remains extremely uncertain. Our new evidence of persistent ultra-weak magnetospheric shielding in the Ediacaran may be considered consistent with the recently hypothesized link between enhanced UV-B radiation in this interval and the subsequent Cambrian evolutionary radiation.
The state of diet-related NCD policies in Afghanistan, Bangladesh, Nepal, Pakistan, Tunisia and Vietnam: a comparative assessment that introduces a ‘policy cube’ approach
Authors: Buse Kent , Aftab Wafa , Akhter Sadika , Phuong Linh Bui , Chemli Haroun , Dahal Minakshi , Feroz Anam , Hofiani Sayad , Pradhan Nousheen Akber , Anwar Iqbal , Skhiri Hajer Aounallah , El Ati Jalila , Giang Kim Bao , Puri Mahesh , Noormal Bashir , Rabbani Fauziah , Hawkes Sarah 
Published: December 2019

Abstract
We assessed the technical content of sugar, salt and trans-fats policies in six countries in relation to the World Health Organization ‘Best Buys’ guidelines for the prevention and control of non-communicable diseases (NCDs). National research teams identified policies and strategies related to promoting healthy diets and restricting unhealthy consumption, including national legislation, development plans and strategies and health sector-related policies and plans. We identified relevant text in relation to the issuing agency, overarching aims, goals, targets and timeframes, specific policy measures and actions, accountability systems, budgets, responsiveness to inequitable vulnerabilities across population groups (including gender) and human rights. We captured findings in a ‘policy cube’ incorporating three dimensions: policy comprehensiveness, political salience and effectiveness of means of implementation, and equity/rights. We compared diet-related NCD policies to human immunodeficiency virus policies in relation to rights, gender and health equity. All six countries have made high-level commitments to address NCDs, but dietary NCDs policies vary and tend to be underdeveloped in terms of the specificity of targets and means of achieving them. There is patchwork reference to internationally recognized, evidence-informed technical interventions and a tendency to focus on interventions that will encounter least resistance, e.g. behaviour change communication in contrast to addressing food reformulation, taxation, subsidies and promotion/marketing. Policies are frequently at the lower end of the authoritativeness spectrum and have few identified budgetary commitments or clear accountability mechanisms. Of concern is the limited recognition of equity and rights-based approaches. Healthy diet policies in these countries do not match the severity of the NCDs burden nor are they designed in such a way that government action will focus on the most critical dietary drivers and population groups at risk. We propose a series of recommendations to expand policy cubes in each of the countries by re-orienting diet-related policies so as to ensure healthy diets for all.
Serum Newborn Screening Blood Metabolites Are not Associated With Childhood-onset Inflammatory Bowel Disease: A Population-based Matched Case-control Study
Authors: Kuenzig M Ellen , Hawken Steven , Wilson Kumanan , Talarico Robert , Chakraborty Pranesh , Sood Manish M , Benchimol Eric I 
Published: December 2019

AbstractBackgroundOriginally used for screening of inborn errors of metabolism, routine metabolite profiles of newborns have also been associated with prematurity and some childhood diseases. We sought to determine whether metabolites measured during routine newborn screening could identify infants who develop inflammatory bowel disease (IBD) in childhood.MethodsWe conducted a population-based matched case-control study using health administrative data from Ontario, Canada. Children born 2006 to 2015 with IBD were identified using a validated algorithm and matched to 5 controls based on birth date, sex, rural/urban household, and mean neighborhood income quintile at birth. Cases and controls were linked deterministically to metabolic profiles from Newborn Screening Ontario. We fit a lasso penalized logistic regression model and used 10-fold cross-validation to obtain internally valid performance measures. Models included metabolites, amino acids, and endocrine markers. Models also included ratios of metabolites, gestational age, birth weight, mode of delivery, age at serum collection, maternal age at delivery, maternal history of IBD, and parity.ResultsThree hundred eight cases of IBD, diagnosed at 5.5 ± 2.8 years, were matched to 1540 controls. No individual metabolites were associated with IBD. The c-statistic was 0.50 for the training data. After 10-fold cross-validation the C statistic was 0.50, indicating no significant association between metabolites and IBD diagnosis.ConclusionsNewborn screening serum metabolites could not identify children who will develop IBD in this population-based cohort. Future studies with an expanded panel of metabolites may provide improved prediction of IBD.
Things and Terms: Relations Between Materiality, Language, and Politics in Post-revolutionary Iran
Authors: Sefat Kusha 
Published: December 2019

AbstractDeparting from the canons of the cultural and material turns, this paper emphasizes the shortcomings that each body of work has shown in addressing political transformations. So doing, it argues that shifting relations between materiality and language occasion different kinds of politics. Specifically, the paper offers a new interpretation of one of the most critical epochs in the political history of modern Iran, by illustrating that the confluence of the material and linguistic worlds in the Islamic Republic during the 1980s, brought about a distinct political field in which relations between words and their material referents became fixed at the level of multitudes. This blocked public processes of performativity and resignification of signs in ways that might have threatened the centrality of the revolutionary leader, Imam Khomeini. What developed was a social milieu in which Khomeini never faced the possibility of defeat in politics.
Metabolic studies reveal that ruminal microbes of adult steers do not degrade rumen-protected or unprotected L-citrulline
Authors: Gilbreath Kyler R , Nawaratna Gayan I , Wickersham Tryon A , Satterfield M Carey , Bazer Fuller W , Wu Guoyao 
Published: December 2019

AbstractIn vitro and in vivo experiments were conducted to determine the metabolism of rumen-protected or unprotected l-citrulline (Cit) plus l-glutamine (Gln) by ruminal microbes. In the in vitro experiment, whole ruminal fluid (3 mL, containing microorganisms) from steers was incubated at 37 ºC with 5 mM Cit plus 6 mM Gln (in a rumen-protected or unprotected form) for 0, 0.5, 2, or 4 h after which times 50 µL samples were collected for AA and ammonia analyses. In the in vivo experiment, at 0.5 h before and 0, 0.5, 1, 2, 4, and 6 h after cannulated adult steers consumed 0.56 kg dried-distillers’ grain mixed with 70 g Cit plus 70 g Gln (in a rumen-protected or unprotected form), samples of ruminal fluid and jugular venous blood were obtained for AA analyses. Results from both in vitro and in vivo experiments demonstrated extensive hydrolysis of rumen-unprotected Gln into glutamate, but little degradation of the rumen-protected Gln or rumen-protected and unprotected Cit by ruminal microbes. Concentrations of Cit and arginine in the plasma of steers consuming rumen-protected or unprotected AA increased at 1 and 2 h after the meal, respectively, when compared with values at 0 h. Collectively, these novel findings indicate that ruminal microbes of adult steers do not degrade extracellular Cit in a rumen-protected or unprotected form. Our results refute the view that all dietary AAs are extensively catabolized by ruminal microorganisms and also have important implications for dietary supplementation with Cit to ruminants to enhance the concentration of arginine in their plasma and their productivity.
Contrast-Enhanced Mammography: A Scientific Review
Authors: Lewin John M , Patel Bhavika K , Tanna Aneri 
Published: December 2019

AbstractIn this paper we provide an overview of contrast-enhanced mammography (CEM) and a review of the published literature in order to provide a picture of the current state of the evidence on the performance of CEM. Clinical research was fairly sparse following the demonstration of the technique in research subjects about 18 years ago, but the number of publications rapidly increased following commercialization 9 years ago, and even more so in the last 5 years. Initial studies compared CEM with mammography, and clearly showed that CEM could detect cancers not visible on mammography. More recent studies have primarily focused on comparing the performance of CEM with contrast-enhanced magnetic resonance imaging (MRI) in selected cohorts. These studies have almost uniformly shown CEM and MRI to have similar sensitivities, with sensitivity and accuracy showing more variability from study to study. With increasing clinical use, a large number of retrospective reviews of CEM have appeared, showing utility of CEM in the diagnostic clinical setting. Most recently, a small number of papers have been published looking at CEM for high-risk and dense breast screening, two potentially large applications of the technique, showing it to outperform mammography in both populations. CEM has clearly been shown to have clinical utility, but more prospective studies, including screening studies, are needed to further evaluate its performance, especially in comparison with MRI.
Implementation of a Diagnostic-Concentration Bioassay for Detection of Susceptibility to Pyrethroids in Soybean Aphid (Hemiptera: Aphididae)
Authors: Menger James , Beauzay Patrick , Chirumamilla Anitha , Dierks Cole , Gavloski John , Glogoza Phillip , Hamilton Krista , Hodgson Erin W , Knodel Janet J , MacRae Ian V , Pezzini Daniela T , Potter Bruce D , Varenhorst Adam J , Koch Robert L 
Published: December 2019

Abstract
Soybean aphid, Aphis glycines Matsumura, remains the most economically damaging arthropod pest of soybean in the midwestern United States and southern Canada. Foliar applications of a limited number of insecticide modes of action have been the primary management tactic, and pyrethroid resistance was documented recently with full concentration–response leaf-dip and glass-vial bioassays. Full concentration–response bioassays can be cumbersome, and a more efficient assessment tool was needed. In this study, we implemented a diagnostic-concentration glass-vial bioassay using bifenthrin and λ-cyhalothrin. Bioassays were conducted with field-collected soybean aphid populations to assess the geographic extent and severity of resistance to pyrethroids. In 2017, 10 of 18 and 11 of 21 field populations tested with bifenthrin and λ-cyhalothrin, respectively, had mean proportion mortalities less than the susceptible laboratory population. In 2018, 17 of 23 and 13 of 23 field populations tested with bifenthrin and λ-cyhalothrin, respectively, had mean proportion mortalities less than the susceptible laboratory population. Populations collected after reported field failures of a pyrethroid insecticide generally had mean proportion mortalities less than the susceptible laboratory population. In both years, there was a strong correlation between chemistries, which suggests cross-resistance between these insecticides. The diagnostic-concentration glass-vial bioassays reported here will provide the foundation for an insecticide resistance monitoring program with the ability to determine practical levels and geographic extent of insecticide resistance.
Flea Beetle (Coleoptera: Chrysomelidae) Populations, Effects of Feeding Injury, and Efficacy of Insecticide Treatments on Eggplant and Cabbage in Southwest Virginia
Authors: Mason James , Michael Alford Adam , Patrick Kuhar Thomas 
Published: December 2019

Abstract
Flea beetles, are common pests of cabbage Brassica oleracea L. (Brassicales: Brassicaceae) and eggplant Solanum melongena L. (Solanales: Solanaceae), but little is known about the flea beetle populations in Virginia, their impact on yield, or the most effective control methods. This research investigates flea beetle populations and the impact of their feeding injury on cabbage and eggplant in Southwest Virginia and determines the most efficacious control methods. In Whitethorne, VA, cabbage and eggplant crops were vacuum sampled weekly throughout two summers (2015, 2016). Crucifer flea beetle, Phyllotreta cruciferae (Goeze) (Coleoptera: Chrysomelidae), and striped flea beetle, Phyllotreta striolata Fabr. (Coleoptera: Chrysomelidae) were found on cabbage; whereas, eggplant flea beetle, Epitrix fucula (Crotch) (Coleoptera: Chrysomelidae), and the tobacco flea beetle, Epitrix hirtipennis (Melsheimer) (Coleoptera: Chrysomelidae) were found on eggplant. To evaluate the impact of flea beetle feeding on these plants flea beetle densities and defoliation were assessed weekly and individual plant, as well as whole plot yields, assessed at harvest. For cabbage, significant yield reductions were observed between 1 and 20% and &gt;60% defoliation. Similarly, significant yield reductions were observed between 41 and 60% and &gt;60% defoliation for eggplant. The efficacy of various insecticides was also evaluated. Soil application of the systemic neonicotinoid dinotefuran, imidacloprid, and the foliar-applied bifenthrin resulted in the fewest beetles, the least amount of leaf defoliation, and the highest yield in cabbage and eggplant. This research helps vegetable growers to better understand the severity of these pests and how to effectively combat them.