bluuebunny/crossref_metadata_2025_split huggingface dataset:
Co-Registered Solid-State 123i-Mibg Spect And Ct Imaging For The Assessment Of Sympathetic Cardiac Innervation In Healthy Individuals And Variation Over Time
Authors: Nikitin Nikita , Minin Stanislav , Stirrup James , Pokushalov Evgeny , Romanov Alexander 
Published: March 2020

Abstract
Background. To evaluate global and regional ventricular and atrial cardiac iodine-123 meta-iodobenzylguanidine ( 123 I-mIBG) uptake and consistency over time in healthy individuals using co-registered SPECT and CT imaging. Fifteen healthy individuals (median age 31 years [26; 41]) were included in the study. All participants underwent CT and subsequent baseline 123 I -mIBG SPECT imaging (early and late acquisition) using a dedicated cardiac solid-state gamma camera. The heart-to-mediastinum (H/M) ratio, wash out rate (WR), summed 123 I-mIBG defect score (SDS) as well as presence and patterns of left atrium (LA) discrete 123 I-mIBG uptake areas were assessed. Follow-up SPECT imaging was acquired 5-7 days after initial procedure. Results. At baseline median H/M ratio on the early and late acquisitions were 1.61 [1.57; 1.71] and 1.68 [1.65; 1.71] respectively, the WR was 22.5% [18.8; 22.8]. Areas of reduced 123 I-mIBG uptake were detected in 60% (9/15) of cases and the median SDS was 1 [0; 2]. No significant changes were observed in global and regional 123 I-mIBG cardiac uptake between baseline and follow-up studies. At baseline 36 discrete uptake areas (DUA) were identified, 16 (44%) of which (median per individual 1 [1;1]) had moderate-high confidence score (CS). 5/16, 4/16, 4/16 and 3/16 moderate-high CS DUAs were located around the left sided-, right sided- PV ostia, LA walls, right atrium (RA) or superior vena cava (SVC), respectively. At follow-up 33 DUAs were identified, 16 (48%) of which (median per individual 1 [1;1], p=0.5 vs baseline) had moderate-high CS. Moderate-high CS discrete uptake areas had generally the same location as on the baseline procedure. Conclusion. Co-registered 123 I-mIBG SPECT and CT imaging demonstrated no significant changes in the global and regional 123 I-mIBG cardiac uptake (ventricular and atrial) over a short time interval in healthy individuals.
Preclinical evaluation of new α-radionuclide therapy targeting LAT1: 2-[211At]astato-α-methyl-L-phenylalanine in tumor-bearing model
Authors: OHSHIMA Yasuhiro , Suzuki Hiroyuki , Hanaoka Hirofumi , Sasaki Ichiro , Watanabe Shigeki , Haba Hiromitsu , Arano Yasushi , Tsushima Yoshito , Ishioka Noriko S. 
Published: March 2020

Abstract
PURPOSE: Targeted α-radionuclide therapy has growing attention as a promising therapy for refractory cancers. However, the application is limited to certain types of cancer. Since L-type amino acids transporter 1 (LAT1) is highly expressed in various human cancers, we prepared LAT1-selective α-emitting amino acid analog, 2-[211At]astato-α-methyl-L-phenylalanine ([211At]-2-AAMP), and evaluated its potential as a therapeutic agent. METHODS: [211At]-2-AAMP was prepared from the stannyl precursor. Stability of [211At]-2-AAMP was evaluated by both in vitro and in vivo. In vitro studies using LAT1 expressing human ovary cancer cell line, SKOV3, were performed for evaluating cellular uptake and cytotoxicity of [211At]-2-AAMP. Biodistribution and therapeutic studies in SKOV3 bearing mice were performed after intravenous injection of [211At]-2-AAMP. RESULTS: [211At]-2-AAMP was stable in murine plasma in vitro and excreted into urine as intact. Cellular uptake of [211At]-2-AAMP was inhibited by treatment with LAT1-selective inhibitor. After 24 hours of incubation, [211At]-2-AAMP suppressed clonogenic growth at 10 kBq/ml, and induced cell death and DNA double strand break at 25 kBq/ml. When injected to mice, [211At]-2-AAMP exhibited the peak accumulation in the tumor at 30 min postinjection, and the radioactivity levels in the tumor retained up to 60 min. The majority of the radioactivity was rapidly eliminated from the body into the urine as an intact form immediately after injection. [211At]-2-AAMP significantly improved the survival of mice (P<0.05) without serious side effects. CONCLUSION: [211At]-2-AAMP showed α-radiation-dependent cellular growth inhibition after taking up via LAT1. Furthermore, [211At]-2-AAMP provided a beneficial effect on survival in vivo. These findings suggest that [211At]-2-AAMP might be useful for treatment of LAT1-positive cancer.
Improving the Hip Fracture Surgical Consenting Process: A Single Centre Multi-Cycle Quality Improvement Process
Authors: Singh Kirit , Assaf Ali , Bayley Morgan , Gillespie Gordon 
Published: March 2020

Abstract
Background Hip fractures are a common acute trauma presentation that if requiring surgery, should be performed within 48 hours. Consenting the patient is an important element of the pathway from admission to operating theatre and errors can lead to delayed procedures, medico-legal exposure and may represent a loss of patient autonomy. The aim of this project was to evaluate areas for improvement in our consenting process for patients with capacity and trial new strategies to determine which achieved the greatest improvement. Methods Following a baseline measurement (n=24), deficiencies were noted in three key process measures of clarity of documentation, failure to record procedure-specific risks and not offering a copy of the consent form to the patient. Two quality improvement cycles then followed using targeted teaching for doctors performing the consenting process. Results Cycle 1 (n=26) following targeted teaching demonstrated improved clarity, with a reduction in abbreviations from 38% to 20%, while more than doubling the documentation of discussion of key procedure-specific risks from 31% to 72%. More patients were offered a copy of their consent form, rising from 12% to 48%. Cycle 2 (n=24) saw the introduction of pre-printed “risk of procedure” stickers. Although clarity measures continued to improve, improvements in pre-procedure risk documentation remained broadly static while the number of forms being offered to patients starkly declined to 8%. Conclusions Our project would suggest that while pre-printed stickers can be useful memory aids, specific teaching on consenting produces the greatest benefit. The usage of such tools should therefore be limited, as adjuncts only to detailed training.
Development of Information Road map for Personalized Colorectal Cancer Screening in Iran
Authors: maserat elham , Mohammadzadeh Zeinab , Zali Mohammad Reza 
Published: March 2020

Abstract
Background A data-driven colorectal cancer screening strategy based on personalized approach can improve health outcomes, facilitate early stratification of at-risk patients and reduce health care costs. This study aims to develop an information road map for personalized colorectal cancer screening in Iran. Methods This study is a Mix-Method Research (MMR) which consisted of three phases: phase I, development of a checklist with 275-items for assessing required data elements of personalized colorectal cancer screening; phase II, situational analysis of colorectal cancer screening dataset according to the checklist; phase III, development of national information road map for personalized colorectal cancer screening with in-depth interview and focus groups. Results Personalized datasets of colorectal cancer screening were defined in four dimensions, including clinical dataset (5 sub-dimensions, 162 items), genetic dataset (2 sub-dimensions, 67 items), demographic dataset (1 sub-dimension, 6 items) and a social determinant dataset (3 sub-dimensions, 40 items). The next step data elements of colorectal cancer screening based on personalized datasets were analyzed. Of the 275-items, only 96 items are recorded. Only 17.8% of clinical dataset of screening program were entered. The highest data elements of clinical dimension were related to pathological datasets (53.6%) in the present screening program. The lowest data elements of the clinical dimensions were related to the clinical history dataset (3.4%). 73% of pedigree data elements and 15.33% of social determinant datasets were entered. In the final step, a national information road map of personalized CRC screening with 6 layers (information leadership, personalized datasets, data integration, data architecture, data descriptor, and screening program layers) was developed. Conclusion Personalized screening based on integration dataset play a key role for the successful implementation of the screening program. Eliminating data deficiencies can improve the quality of documentation and may lead to improved screening performance. Therefore, standard datasets and indicators can help to identify information gaps and facilitate precise decision-making. Entering data was inadequate and poor in this study. Implementation of national road map can assist to improve the quality of data in personalized screening.
Helicobacter pylori infection as a risk factor for diabetes: A Meta-analysis of Case-Control Studies
Authors: Mansori Kamyar , Moradi Yousef , Naderpour Sara , Rashti Roya , Moghaddam Ali Baradaran , Saed Lotfolah , Mohammadi Hedye 
Published: March 2020

Abstract
Background There are several studies with varied and mixed results about the possible relationship between H. pylori and diabetes. Therefore, this current meta-analysis performed to determine the association between H. pylori infection and the risk of diabetes mellitus. Methods A systematic literature searches of international databases, including Medline (PubMed), Web of Sciences, Scopus, EMBASE, and CINHAL (January 1990–March 2019) was conducted to identify studies investigating the relationship between H. pylori infection and diabetes mellitus. Only case–control studies were analyzed using odds ratio (OR) with 95 % confidence intervals (CIs). Stratified and subgroup analyses were performed to explore heterogeneity between studies and assess effects of study quality. Logarithm and standard error logarithm odds ratio (OR) were also used for meta-analysis. Results A total of 41 studies involving 9559 individuals (case; 4327 and control; 5232) were analyzed. The pooled estimate of the association between H. pylori infection with diabetes was OR=1.27 (95% CI 1.11 to 1.45, P = 0.0001, I2 = 86.6%). The effect of H. pylori infection on diabetes mellitus (both types), type 1 and type 2 diabetes was 1.17 (95% CI 0.94 to 1.45), 1.19 (95% CI 0.98 to 1.45), and 1.43 (95% CI 1.11 to 1.85) respectively. Subgroup analysis by the geographical regions showed in Asian population risk of the effect of H. pylori infection on diabetes was slightly higher than other population.Conclusion In overall a positive association between H. pyloriinfection and diabetes mellitus was found.
Epidemiology of Gastroesophageal Reflux Disease in Iran: A Systematic Review and Meta-analysis
Authors: Karimian Mohammad , Salamati Majid , Shafiee Elham , Azami Milad 
Published: March 2020

Abstract
Introduction : Gastroesophageal reflux disease (GERD), which leads to acid reflux into the esophagus, is a common gastrointestinal disorder. Several studies have shown the prevalence of GERD in Iranian population, but their evidence is contradictory. Therefore, the present study was conducted to investigate the epidemiology of GERD in Iran. Methods: The entire steps of this systematic review and meta-analysis were based on the MOOSE protocol, and the results were accordance with the PRISMA guideline. To find potentially relevant published articles, comprehensive search was done on international online databases Scopus, Science Direct, EMBASE, PubMed/Medline, CINAHL, EBSCO, Cochrane Library (Cochrane Database of Systematic Reviews - CDSR), Web of Science (ISI) and Iranian online databases IranDoc, (SID), Magiran, RICST, Iranian National Library, and Barakat Knowledge Network System and the Google Scholar search engine in June 2019. Cochran test and I 2 index were used to assess the heterogeneity of the studies. Data were analyzed using Comprehensive Meta-Analysis (CMA) software. Results: The daily, weekly, monthly, and overall prevalence of GERD symptoms in Iranian population was 5.64% (95%CI [confidence interval]: 3.77%-8.35%; N=66,398), 12.50% (95%CI: 9.63%-16.08%; N=110,388), 18.62% (95%CI: 12.90%-26.12%; N= 70,749) and 43.07% (95%CI: 35.00%-51.53%; N=73,189), respectively. The daily, weekly, monthly, and overall prevalence of heartburn in Iranian population was 2.46% (95%CI: 0.93%-6.39%; N=18,774), 9.52% (95%CI: 6.16%-14.41%; N=54,125), 8.19% (95%CI: 2.42%-24.30%; N=19,363) and 23.20% (95%CI: 13.56%-36.79%; N=26,543), respectively. The daily, weekly, monthly, and overall prevalence of regurgitation in Iranian population was 4.00% (95%CI: 1.88%-8.32%; N=18,774), 9.79% (95%CI: 5.99%-15.60%; N=41,140), 13.76% (95%CI: 6.18%-44.31%; N=19,363) and 36.53% (95%CI: 19.30%-58.08%; N=21,174), respectively. Conclusion: The present meta-analysis provides comprehensive and useful information on the epidemiology of GERD in Iran for policy-makers and health care providers. This study showed a high prevalence of GERD in Iran. Therefore, effective measures on GERD-related factors such as lifestyle can be among the health policies of Iran.
Antibodies from multiple sclerosis brain identified Epstein-Barr virus nuclear antigen 1 &amp; 2 epitopes which are recognized by oligoclonal bands
Authors: Wang Zhe , Kennedy Peter GE , Dupree Cecily , Wang Min , Lee Catherin , Pointon Tiffany , Langford T. Dianne , Graner Michael , Yu Xiaoli 
Published: March 2020

Abstract
Background Multiple sclerosis (MS) is a chronic inflammatory demyelinating disease of the central nervous system (CNS), the etiology of which is poorly understood. The most common laboratory abnormality associated with MS is increased intrathecal IgG synthesis and the presence of oligoclonal bands (OCBs) in the brain and cerebrospinal fluid (CSF). However, the major antigenic targets of these antibody responses are unknown. The risk of MS is increased after infectious mononucleosis (IM) due to EBV infection, and MS patients have higher serum titers of anti-EBV antibodies than control populations. Objectives To identify disease-relevant epitopes of IgG antibodies in MS. Methods We screened phage-displayed random peptide libraries (12-mer) with total IgG antibodies purified from the brain of a patient with acute MS. We identified and characterized the phage peptides for binding specificity to intrathecal IgG from patients with MS and from controls by ELISA, phage-mediated Immuno-PCR, and isoelectric focusing. Results Two phage peptides were identified that share sequence homologies with EBV nuclear antigens 1 and 2 (EBNA1 and EBNA2), respectively. The specificity of the EBV epitopes found by panning with MS brain IgG was confirmed by ELISA and competitive inhibition assays. Using a highly sensitive phage-mediated immuno-PCR assay, we determined specific bindings of the two EBV epitopes to IgG from CSF from 46 MS and 5 inflammatory control (IC) patients. MS CSF IgG have significantly higher bindings to EBNA1 epitope than to EBNA2 epitope, whereas EBNA1 and EBNA2 did not significantly differ in binding to IC CSF IgG. Further, the EBNA1 epitope was recognized by OCBs from multiple MS CSF as shown in blotting assays with samples separated by isoelectric focusing. Conclusions The EBNA1 epitope is reactive to MS intrathecal antibodies corresponding to oligoclonal bands. This reinforces the potential role of EBV in the etiology of MS.
Person-Centred, Occupation Based Intervention Program Supported with Problem-Solving Therapy for Type 2 Diabetes: a randomized controlled trial
Authors: Ağce Zeynep Bahadır , Ekici Gamze 
Published: March 2020

Abstract
Background: Individuals with diabetes mellitus both have difficulty in solving problems in daily life and have similar difficulties with self-care regimens. We examined the effect of occupation-based intervention supported with problem-solving therapy of individuals with type 2 diabetes mellitus on participation and satisfaction of meaningful occupations, diabetes-related psychosocial self-efficacy, preferred coping strategies and individual well-being. Methods: This study was planned as a single-blind, randomized controlled study with 3-months’ follow-up involving sixty-seven adults with type 2 diabetes. The Canadian Occupational Performance Measure, the Diabetes Empowerment Scale, the Brief COPE and the Five-item World Health Organization Well-Being Index were used. The intervention was conducted in 6 modules, and each implemented about 60 minutes and for 1 session per week. The six modules included evaluations, diabetes education, and problem-solving therapy. The most important feature of the program is its focus on meaningful occupations for the person and provided an opportunity for the participants to apply problem-solving therapy through valued occupation. Differences between groups were analysed with the Mann Whitney U test, and the Friedman test was used to calculate group-time interaction differences (i.e. baseline, after six modules and after three months). Results: Individuals with type 2 diabetes mellitus in both groups identified the most significant meaningful activity problems in self-care as personal care. Significant improvement was reported in the intervention group when compared to the control group regarding the participation in meaningful occupation, the satisfaction of performance, psychological self-efficacy and well-being results (p<0.001) after the six modules and 3 months follow-up. The participants' use of the effective coping strategies, active coping and acceptance strategies, self-efficacy as revealed results showed improvement in favor of the intervention group (p<0.05). The intervention group showed significantly improving between measurements at baseline and after three months of modules except for ineffective coping style (p<0.001). Conclusions: The person-centred occupation-based problem-solving therapy encourages participation in meaningful occupations, positive effects on psychological self-efficacy, and improves effective coping styles and well-being of type 2 diabetes mellitus. Problem-solving therapies that incorporate individuals’ priorities via meaningful occupation could be used to supported to lead a meaningful life of individuals of type 2 diabetes mellitus. Trial Registration: ClinicalTrials.gov Identifier: NCT03783598. Retrospectively Registered. First Posted-December 21, 2018, Last Update Posted-February 18, 2020. Key words: Diabetes mellitus, Person-centred, Occupation-based, Problem-solving, Psychosocial self-efficacy, Coping, Well-being, Quality of life, Occupational therapy
Beauveria bassiana ERL836 and JEF-007 with similar virulence show different gene expression when infecting western flower thrips, Frankniella occidentalis
Authors: Kim Jae Su , Kim Sihyeon , Kim Jong Cheol , Lee Se Jin , Lee Mi rong , Park So Eun , Li Dongwei , Baek Sehyeon , Shin Tae Young 
Published: March 2020

Abstract
Background: Insect-killing fungal species, Beauveria bassiana, is as an environment-friendly pest management tool, and many isolates are on the track of industrialization. However, some of B. bassiana isolates show similar morphology and virulence against insect pests, and so it is hard to differentiate them. Herein we used two patented isolates, ERL836 and JEF-007, and investigated their virulence against western flower thrips, Frankliniella occidentalis, and further analyzed genome structures and transcriptional responses when infecting the thrips to see possible differences.Results: The two isolates showed no significant differences in fungal growth, conidial production, virulence against thrips, and they were structurally similar in genome. But, in transcription level, ERL836 appeared to infect thrips easily, while JEF-007 appeared to have more difficulty. In the GO analysis of ERL836 DEGs (differentially expressed genes), the number of up-regulated genes was much larger than that of down-regulated genes, when compared to JEF-007 DEGs (more genes down-regulated). Interestingly, in the enrichment analysis using shared DEGs between two infecting isolates, plasma membrane-mediated transporter activity and fatty acid degradation pathway including cytochrome P450 were more active in infecting ERL836.Conclusion: The two B. bassiana isolates had similar morphology and virulence as well as genome structure, but in transcription level they differently infected western flower thrips. This comparative approach using shared DEG analysis could be easily applied to characterize the difference of the two B. bassiana isolates, JEF-007 and ERL836.
Integrated analysis of autophagy-related genes(ATGs) reveals the prognostic value of oral squamous cell carcinoma
Authors: Huang Guangzhao , Li Zhi-yun , Rao Yu , Lv Xiao-zhi 
Published: March 2020

Abstract
Background: Increasing evidence demonstrated that autophagy paly a crucial role in initiation and progression of OSCC. The aim of this study was to explore the prognostic value of autophagy-related genes(ATGs) in patients with OSCC. RNA-seq and clinical data were downloaded from TCGA database following extrating ATGs expression profiles. Then, differentially expressed analysis was performed in R software EdgeR package, and the potential biological function of differentially expressed ATGs were explored by GO and KEGG enrichment analysis. Furthermore, a risk score model based on ATGs was constructed to predict the overall survival. Moreover, univariate, multivariate cox regression and survival analysis were used to select autophagy related biomarkers which were identified by RT-qPCR in OSCC cell lines, OSCC tissues and matched normal mucosal tissues. Results: Total of 232 ATGs were extrated and 37 genes were differentially expressed in OSCC. GO and KEGG analysis indicated that these differentially expressed genes were mainly located in autophagosome membrane, and associated with apoptosis, platinum drug resistance, ErbB signaling pathway and TNF signaling pathway. Furthermore, a risk score model including 9 variables was constructed and subsequently identified with univariate, multivariate cox regression, survival analysis and Receiver Operating Characteristic curve(ROC). Moreover, ATG12 and BID were identified as potential autophagy related biomakers. Conclusion: This study successfully constructed a risk model to predict the prognosis of patients with OSCC, and the risk score may be as a independent prognostic biomarker in OSCC. ATG12 and BID were identified as potential biomarkers in tumor diagnosis and treatment of OSCC.
Host DNA contents in fecal metagenomics as a biomarker for intestinal diseases and effective treatment
Authors: Jiang Puzi , Lai Senying , Wu Sicheng , Zhao Xing-Ming , Chen Wei-Hua 
Published: March 2020

Abstract
BackgroundCompromised intestinal barrier (CIB) has been associated with many enteropathies, including colorectal cancer (CRC) and inflammatory bowel disease (IBD). We hypothesized that CIB could lead to increased host-derived contents including epithelial cells into the gut, change its physio-metabolic properties, and globally alter microbial community and metabolic capacities. ResultsConsistently, we found host DNA contents (HDCs), calculated as the percentage of metagenomic sequencing reads mapped to the host genome, were significantly elevated in patients of CRC and Crohn’s disease (CD). Consistent with our hypothesis, we found that HDC correlated with microbial- and metabolic-biomarkers of these diseases, contributed significantly to machine-learning models for patient stratification and was consequently ranked as a top contributor. CD patients with treatment could partially reverse the changes of many CD-signature species over time, with reduced HDC and fecal calprotectin (FCP) levels. Strikingly, HDC showed stronger correlations with the reversing changes of the CD-related species than FCP, and contributed great in classifying treatment responses, suggesting that it was also a biomarker for effective treatment. ConclusionsTogether, we identified a novel biomarker from fecal metagenomics for intestinal diseases and effective treatment; our results also suggested that host-derived contents may have greater impact on gut microbiota than previously anticipated.