bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impact of genome duplication on secondary metabolite composition in non-cultivated species: a systematic meta-analysis
Authors: Gaynor Michelle L , Lim-Hing Simone , Mason Chase M 
Published: June 2020

Abstract

Background and Aims
Whole-genome duplication is known to influence ecological interactions and plant physiology; however, despite abundant case studies, much is still unknown about the typical impact of genome duplication on plant secondary metabolites (PSMs). In this study, we assessed the impact of polyploidy events on PSM characteristics in non-cultivated plants.

Methods
We conducted a systematic review and meta-analysis to compare composition and concentration of PSMs among closely related plant species or species complexes differing in ploidy level.

Key Results
We assessed 53 studies that focus on PSMs among multiple cytotypes, of which only 14 studies compared concentration quantitatively among cytotypes. We found that whole-genome duplication can have a significant effect on PSM concentration; however, these effects are highly inconsistent.

Conclusion
Overall, there was no consistent effect of whole-genome duplication on PSM concentrations or profiles.
Words Go Together Like ‘Bread and Butter’: The Rapid, Automatic Acquisition of Lexical Patterns
Authors: Conklin Kathy , Carrol Gareth 
Published: June 2020

Abstract
While it is possible to express the same meaning in different ways (‘bread and butter’ versus ‘butter and bread’), we tend to say things in the same way. As much as half of spoken discourse is made up of formulaic language or linguistic patterns. Despite its prevalence, little is known about how the processing system treats novel patterns and how rapidly a sensitivity to them arises in natural contexts. To address this, we monitored native English speakers’ eye movements when reading short stories containing existing (conventional) patterns (‘time and money’), seen once, and novel patterns (‘wires and pipes’), seen one to five times. Subsequently, readers saw both existing and novel phrases in the reversed order (‘money and time’; ‘pipes and wires’). In four to five exposures, much like existing lexical patterns, novel ones demonstrate a processing advantage. Sensitivity to lexical patterns—including the co-occurrence of lexical items and the order in which they occur—arises rapidly and automatically during natural reading. This has implications for language learning and is in line with usage-based models of language processing.
vmPFC Drives Hippocampal Processing during Autobiographical Memory Recall Regardless of Remoteness
Authors: McCormick Cornelia , Barry Daniel N , Jafarian Amirhossein , Barnes Gareth R , Maguire Eleanor A 
Published: June 2020

Abstract
Our ability to recall past experiences, autobiographical memories (AMs), is crucial to cognition, endowing us with a sense of self and underwriting our capacity for autonomy. Traditional views assume that the hippocampus orchestrates event recall, whereas recent accounts propose that the ventromedial prefrontal cortex (vmPFC) instigates and coordinates hippocampal-dependent processes. Here we sought to characterize the dynamic interplay between the hippocampus and vmPFC during AM recall to adjudicate between these perspectives. Leveraging the high temporal resolution of magnetoencephalography, we found that the left hippocampus and the vmPFC showed the greatest power changes during AM retrieval. Moreover, responses in the vmPFC preceded activity in the hippocampus during initiation of AM recall, except during retrieval of the most recent AMs. The vmPFC drove hippocampal activity during recall initiation and also as AMs unfolded over subsequent seconds, and this effect was evident regardless of AM age. These results recast the positions of the hippocampus and the vmPFC in the AM retrieval hierarchy, with implications for theoretical accounts of memory processing and systems-level consolidation.
Phage Therapy for Limb-threatening Prosthetic KneeKlebsiella pneumoniaeInfection: Case Report and In Vitro Characterization of Anti-biofilm Activity
Authors: Cano Edison J , Caflisch Katherine M , Bollyky Paul L , Van Belleghem Jonas D , Patel Robin , Fackler Joseph , Brownstein Michael J , Horne Bri’Anna , Biswas Biswajit , Henry Matthew , Malagon Francisco , Lewallen David G , Suh Gina A 
Published: June 2020

AbstractBackgroundProsthetic joint infection (PJI) is a potentially limb-threatening complication of total knee arthroplasty. Phage therapy is a promising strategy to manage such infections including those involving antibiotic-resistant microbes, and to target microbial biofilms. Experience with phage therapy for infections associated with retained hardware is limited. A 62-year-old diabetic man with a history of right total knee arthroplasty 11 years prior who had suffered multiple episodes of prosthetic knee infection despite numerous surgeries and prolonged courses of antibiotics, with progressive clinical worsening and development of severe allergies to antibiotics, had been offered limb amputation for persistent right prosthetic knee infection due to Klebsiella pneumoniae complex. Intravenous phage therapy was initiated as a limb-salvaging intervention.MethodsThe patient received 40 intravenous doses of a single phage (KpJH46Φ2) targeting his bacterial isolate, alongside continued minocycline (which he had been receiving when he developed increasing pain, swelling, and erythema prior to initiation of phage therapy). Serial cytokine and biomarker measurements were performed before, during, and after treatment. The in vitro anti-biofilm activity of KpJH46Φ2, minocycline and the combination thereof was evaluated against a preformed biofilm of the patient’s isolate and determined by safranin staining.ResultsPhage therapy resulted in resolution of local symptoms and signs of infection and recovery of function. The patient did not experience treatment-related adverse effects and remained asymptomatic 34 weeks after completing treatment while still receiving minocycline. A trend in biofilm biomass reduction was noted 22 hours after exposure to KpJH46Φ2 (P = .063). The addition of phage was associated with a satisfactory outcome in this case of intractable biofilm-associated prosthetic knee infection. Pending further studies to assess its efficacy and safety, phage therapy holds promise for treatment of device-associated infections.
Global evaluation of echocardiography in patients with COVID-19
Authors: Dweck Marc R , Bularga Anda , Hahn Rebecca T , Bing Rong , Lee Kuan Ken , Chapman Andrew R , White Audrey , Salvo Giovanni Di , Sade Leyla Elif , Pearce Keith , Newby David E , Popescu Bogdan A , Donal Erwan , Cosyns Bernard , Edvardsen Thor , Mills Nicholas L , Haugaa Kristina 
Published: June 2020

Abstract

Aims
To describe the cardiac abnormalities in patients with COVID-19 and identify the characteristics of patients who would benefit most from echocardiography.

Methods and results
In a prospective international survey, we captured echocardiography findings in patients with presumed or confirmed COVID-19 between 3 and 20 April 2020. Patient characteristics, indications, findings, and impact of echocardiography on management were recorded. Multivariable logistic regression identified predictors of echocardiographic abnormalities. A total of 1216 patients [62 (52–71) years, 70% male] from 69 countries across six continents were included. Overall, 667 (55%) patients had an abnormal echocardiogram. Left and right ventricular abnormalities were reported in 479 (39%) and 397 (33%) patients, respectively, with evidence of new myocardial infarction in 36 (3%), myocarditis in 35 (3%), and takotsubo cardiomyopathy in 19 (2%). Severe cardiac disease (severe ventricular dysfunction or tamponade) was observed in 182 (15%) patients. In those without pre-existing cardiac disease (n = 901), the echocardiogram was abnormal in 46%, and 13% had severe disease. Independent predictors of left and right ventricular abnormalities were distinct, including elevated natriuretic peptides [adjusted odds ratio (OR) 2.96, 95% confidence interval (CI) 1.75–5.05) and cardiac troponin (OR 1.69, 95% CI 1.13–2.53) for the former, and severity of COVID-19 symptoms (OR 3.19, 95% CI 1.73–6.10) for the latter. Echocardiography changed management in 33% of patients.

Conclusion
In this global survey, cardiac abnormalities were observed in half of all COVID-19 patients undergoing echocardiography. Abnormalities were often unheralded or severe, and imaging changed management in one-third of patients.
Incident atrial fibrillation and risk of psychoactive drug redemptions and psychiatric hospital contacts: a Danish Nationwide Register-based Follow-up Study
Authors: Hagengaard Louise , Polcwiartek Christoffer , Andersen Mikkel Porsborg , Sessa Maurizio , Krogager Maria Lukacs , Gislason Gunnar , Schou Morten , Torp-Pedersen Christian , Søgaard Peter , Kragholm Kristian Hay 
Published: June 2020

Abstract

Aims
To investigate whether incident atrial fibrillation or flutter (AF) diagnosis increases the risk of psychiatric outcomes compared with the general population.

Methods and results
First-time AF patients and population controls naive to psychiatric disease or filled prescriptions for psychotropic drugs were identified in Danish nationwide registries during 2005–14. AF patients were matched 1:2 with exposure density matching. Patients and controls were compared for 1-year cumulative incidences of depression, anxiety, and stress disorders, and for filled drug prescriptions for antidepressant, anxiolytic, selected antipsychotics, and hypnotics. Lastly, we examined 1-year cumulative incidences of a composite endpoint of the above-mentioned diagnoses or drug redemptions. We included 146 377 AF patients and 292 754 matched controls, 55% men and median age 74 (25–75% 65–82) years. AF patients had significantly higher cumulative incidences of composite endpoints. Furthermore, filled prescriptions for anxiolytics and hypnotics were significantly higher for AF patients compared with healthy population controls. The cumulative incidence of the composite endpoint was significantly higher in AF patients relative to controls 11.1% vs. 8.3%. For the composite endpoint, a significantly higher risk was apparent both in unadjusted (HR: 2.76, 95% CI: 2.67–2.85) and adjusted (HR: 2.51, 95% CI: 2.43–2.60) models for AF patients vs. controls in the first 3 months after study inclusion.

Conclusion
First-time AF patients were significantly more likely to have psychiatric outpatient or hospital contacts and to fill prescriptions for psychotropic drugs compared with healthy population controls. The risk was significantly elevated only during the first 3 months after AF diagnosis.
Host genotype explains rhizospheric microbial community composition: the case of wild cotton metapopulations (Gossypium hirsutum L.) in Mexico
Authors: Hernández-Terán Alejandra , Navarro-Díaz Marcelo , Benítez Mariana , Lira Rafael , Wegier Ana , Escalante Ana E 
Published: June 2020

ABSTRACTThe rhizosphere provides several benefits to the plant host being a strong determinant for its health, growth and productivity. Nonetheless, the factors behind the assembly of the microbial communities associated with the rhizosphere such as the role of plant genotypes are not completely understood. In this study, we tested the role that intraspecific genetic variation has in rhizospheric microbial community assemblages, using genetically distinct wild cotton populations as a model of study. We followed a common garden experiment including five wild cotton populations, controlling for plant genotypes, environmental conditions and soil microbial community inoculum, to test for microbial differences associated with genetic variation of the plant hosts. Microbial communities of the treatments were characterized by culture-independent 16S rRNA gene amplicon sequencing with Illumina MiSeq platform. We analyzed microbial community diversity (alpha and beta), and diversity structure of such communities, determined by co-occurrence networks. Results show that different plant genotypes select for different and specific microbial communities from a common inoculum. Although we found common amplicon sequence variants (ASVs) to all plant populations (235), we also found unique ASVs for different populations that could be related to potential functional role of such ASVs in the rhizosphere.
The virulence factor urease and its unexplored role in the metabolism of Cryptococcus neoformans
Authors: Toplis Barbra , Bosch Caylin , Schwartz Ilan S , Kenyon Chris , Boekhout Teun , Perfect John R , Botha Alfred 
Published: June 2020

ABSTRACT
Cryptococcal urease is believed to be important for the degradation of exogenous urea that the yeast encounters both in its natural environment and within the human host. Endogenous urea produced by the yeast's own metabolic reactions, however, may also serve as a substrate for the urease enzyme. Using wild-type, urease-deletion mutant and urease-reconstituted strains of Cryptococcus neoformans H99, we studied reactions located up- and downstream from endogenous urea. We demonstrated that urease is important for cryptococcal growth and that, compared to nutrient-rich conditions at 26°C, urease activity is higher under nutrient-limited conditions at 37°C. Compared to cells with a functional urease enzyme, urease-deficient cells had significantly higher intracellular urea levels and also showed more arginase activity, which may act as a potential source of endogenous urea. Metabolic reactions linked to arginase were also affected, since urease-positive and urease-negative cells differed with respect to agmatinase activity, polyamine synthesis, and intracellular levels of proline and reactive oxygen species. Lastly, urease-deficient cells showed higher melanin levels at 26°C than wild-type cells, while the inverse was observed at 37°C. These results suggest that cryptococcal urease is associated with the functioning of key metabolic pathways within the yeast cell.
Comparative studies of egg parasitoids of the pine processionary moth (Thaumetopoea pityocampa,Den. & Schiff.) in historic and expansion areas in France and Bulgaria
Authors: Georgiev Georgi , Rousselet Jérôme , Laparie Mathieu , Robinet Christelle , Georgieva Margarita , Zaemdzhikova Gergana , Roques Alain , Bernard Alexis , Poitou Laura , Buradino Maurane , Kerdelhue Carole , Rossi Jean-Pierre , Matova Maria , Boyadzhiev Peter , Mirchev Plamen 
Published: June 2020

AbstractTo investigate enemy pressure across the range of the pine processionary moth (Thaumetopoea pityocampa, Den. & Schiff.) as it spreads northwards in Europe because of climate change, a survey of 206 egg batches from historical and newly colonized areas at both the westernmost and the easternmost ends of the front was carried out in 2016–2018. Out of them, 97 egg batches were collected from nine locations in three regions in France and 109 from four localities in two regions in Bulgaria, both within historical and newly colonized ranges of the pest. The average number of eggs per batch collected in Bulgaria (226.6 ± 43.2) was higher than that in France (194.3 ± 50.1). However, the hatching percentage was higher in French samples, varying from 69.8 to 95.7, vs 49.8 to 85.2 per cent in Bulgarian samples. Four primary parasitoids (Ooencyrtus pityocampae, Baryscapus servadeii, Anastatus bifasciatus, Trichogramma sp.) and a hyperparasitoid (Baryscapus transversalis, found only in two regions of the historical range in Bulgaria) were identified. The oligophagous species B. servadeii was present at all sites within the historical range of the pest. In newly colonized areas, the impact of primary parasitoids on the host was distinctly low, suggesting that they lag behind the range expansion of pine processionary moth. In France, the most abundant species in the T. pityocampa parasitoid complex were B. servadeii in Ré Island and Orléans (97.3 and 87.4 per cent, respectively), and Trichogramma sp. (99.7 per cent) in a newly colonized locality in Fréhel. Ooencyrtus pityocampae prevailed in three of four Bulgarian localities (72.1 per cent in Sandanski, 89.7 per cent in Maglizh and 65.7 per cent in Sladak kladenets), whereas B. servadeii was the most abundant in Gega (75.4 per cent). Mortality of B. servadeii and O. pityocampae caused by the hyperparasitoid B. transversalis amounted to 4.8–6.2 per cent. The impact of predators on the pine processionary moth in the egg stage was negligible at most sites, reaching 12.5 per cent in only one site (Maglizh).
Chemoprevention of esophageal adenocarcinoma
Authors: Snider Erik J , Kaz Andrew M , Inadomi John M , Grady William M 
Published: June 2020

Abstract
Esophageal adenocarcinoma (EAC) is a major cause of cancer-related death, particularly in Western populations, and is rapidly rising in Asian populations at this time. Virtually all EACs develop from the precursor lesion Barrett’s esophagus (BE), which is the most significant risk factor for EAC. However, the rates of progression from BE to EAC are low and patients with BE are asymptomatic. Thus, any strategy for EAC prevention must carry a low risk of harm in order to be clinically useful. Since current EAC-screening and BE-surveillance methods carry some procedural risk and are burdensome, there is an opportunity for chemoprevention, i.e. medications or dietary factors that may prevent BE from progressing to EAC. A variety of candidate chemoprevention therapies have been assessed to date. Proton-pump inhibitors (PPIs) are the best studied and have modest EAC-chemoprevention efficacy in BE patients, with a recent randomized trial showing that high-dose PPI may be more effective than low-dose PPI. Aspirin and other non-steroidal anti-inflammatory drugs have moderate quality observational and randomized-trial evidence for preventing progression of BE to EAC, but their risks for harm have precluded their routine clinical use. Other therapies (statins, metformin, female sex hormones) generally do not have strong evidence to support their use in EAC chemoprevention. Although progress has been made in this field, there is still a need for more effective and safe chemoprevention therapies for EAC.
National Partnership to Improve Dementia Care in Nursing Homes Campaign: State and Facility Strategies, Impact, and Antipsychotic Reduction Outcomes
Authors: Crystal Stephen , Jarrín Olga F , Rosenthal Marsha , Hermida Richard , Angell Beth 
Published: June 2020

Abstract

Background and Objectives
Antipsychotic medications have been widely used in nursing homes to manage behavioral and psychological symptoms of dementia, despite significantly increased mortality risk. Use grew rapidly during the 2000s, reaching 23.9% of residents by 2011. A national campaign for safer dementia care in U.S. nursing homes was launched in 2012, with public reporting of quality measures, increased regulatory scrutiny, and accompanying state and facility initiatives. By the second quarter of 2019, use had declined by 40.1% to 14.3%. We assessed the impact of state and facility initiatives during the Campaign aimed at encouraging more-judicious prescribing of antipsychotic medications.

Research Design and Methods
Our mixed-methods strategy integrated administrative and clinical data analyses with state and facility case studies.

Results
Results suggest that substantial change in prescribing is achievable through sustained, data-informed quality improvement initiatives integrating educational and regulatory interventions, supported by public quality reporting. Adequate staffing, particularly of registered nurses, is key to support individualized management of symptoms through nonpharmacological strategies. Case study results suggest that state and facility initiatives during the campaign achieved considerable buy-in for the goal of more conservative prescribing, through a social process of normalization. Reporting and reduction of antipsychotic use was not followed by increases in sedative-hypnotic medication use. Rather, sedative-hypnotic use declined in tandem with antipsychotic reduction, suggesting that increased attention to prescribing patterns led to more cautious use of other risky psychotropic medications.

Discussion and Implications
Quality improvement initiatives to change entrenched but problematic clinical practices face many barriers to success, including provider-level inertia; perceptions that alternatives are not available; and family and staff resistance. Nevertheless, systemic change is possible through concerted, collaborative efforts that touch prescribing practices at multiple points; integrate educational and regulatory influences; activate local and state champions for improvement; foster reputational influences through public reporting and benchmarking; and support a social process of normalization of preferred care processes as a best practice that is in the interest of patients.
Fromcontrollingtoconnecting: M’Wikwedong as a place of urban Indigenous health promotion in Canada
Authors: Sanchez-Pimienta Carlos E , Masuda Jeff ,   M'Wikwedong Indigenous Friendship Centre
Published: June 2020

AbstractIn recent years, health promotion has come under critique for being framed according to the contexts and priorities of Western communities, with the notion of ‘control’ underpinning much of its theoretical and practical development. Ceding space to Indigenous voices and knowledge is one way forward to overcoming this limitation and decolonizing the field. This paper reports on insights gained from a participatory digital storytelling project focused on Indigenous health promotion that took place at M’Wikwedong Indigenous Friendship Centre in the city of Owen Sound, Canada. The research team was formed by M’Wikwedong’s Executive Director, five Indigenous youth and two university researchers. We co-created data through an 8-month digital storytelling process that involved 13 weekly research meetings, the creation of 4 digital stories and video screenings. We analysed data from seven group interview transcriptions, field notes and video transcripts through qualitative coding and theme building. The four themes we identified speak to the ways M’Wikwedong reinforced connections to youth, their sense of self, place in the city and Indigenous cultures. From our findings, we theorize that egalitarianism of knowledge, restoring balance in relationships and Indigenous leadership are core components of an ‘ethos of connection’ that underlies Indigenous health promotion. The ‘ethos of connection’ challenges Western notions of ‘control’ and brings attention to the unique expertise and practices of urban Indigenous communities and organizations as a primary basis for health promotion.