bluuebunny/crossref_metadata_2025_split huggingface dataset:
Antiproliferative Activity of Functionalized Histidine‐derived Au(I) bis‐NHC Complexes for Bioconjugation
Authors: Jakob Christian H. G. , Dominelli Bruno , Hahn Eva M. , Berghausen Tobias O. , Pinheiro Teresa , Marques Fernanda , Reich Robert M. , Correia João D. G. , Kühn Fritz E. 
Published: June 2020

AbstractA series of histidine derived Au(I) bis‐NHC complexes bearing different ester, amide and carboxylic acid functionalities as well as wingtip substituents is synthesized and characterized. The stability in aqueous media, in vitro cytotoxicity in a set of cancer cell lines (MCF7, PC3 and A2780/A2780cisR) along with the cellular uptake are evaluated. Stability tests suggest hydrolysis of the ester within 8 h, which might lead to deactivation. Furthermore, the bis‐NHC system shows a sufficient stability against cysteine and the thiol containing peptide GSH. The benzyl ester and amide show the highest activity comparable to the benchmark compound cisplatin, with the ester only displaying a slightly lower cytotoxicity than the amide. A cellular uptake study revealed that the benzyl ester and the amide could have different intracellular distribution profiles but both complexes induce perturbations of the cellular physiological processes. The simple modifiability and high stability of the complexes provides a promising system for upcoming post modifications to enable targeted cancer therapy.
A sensitive fluorescent probe based on dithizone‐capped ZnS quantum dots for quercetin determination in biological samples
Authors: Sadeghi Susan , Hosseinpour‐Zaryabi Mohadeseh 
Published: June 2020

AbstractA simple turn on/off fluorescence approach based on dithizone‐capped ZnS quantum dots (ZnS@DZ QDs) with the help of lead ions as a fluorescent probe for the quantitative determination of quercetin is reported. The interaction of lead ions with dithizone led to the formation of a rigid structure on the surface of ZnS@DZ QDs and turned on the fluorescence intensity of the QDs. After addition of quercetin to this probe and interaction with lead ions, the fluorescence emission turned off. Concerning the quenching fluorescence intensity of ZnS@DZ QDs/Pb2+ QDs probe induced by the target, under the optimum conditions, the probe enabled detection of quercetin in the concentration range from 0.54 μM to 21.7 μM with a correlation coefficient of 0.993 and detection limit of 0.25 μM. The present probe was applied successfully to the determine quercetin as a nutritional biomarker in human serum and 24‐h urine samples.
POTEE stimulates the proliferation of pancreatic cancer by activating the PI3K/Akt/GSK‐3β/β‐catenin signaling
Authors: Hao Qingya , Gao Lihua , Niu Wenyang , Chen Liang , Zhang Peng , Chen Zhong 
Published: June 2020

AbstractTo explore the role of POTEE in the malignant development of pancreatic cancer and the possible mechanism. POTEE levels in pancreatic cancer samples were detected. The relationship between POTEE level and clinical data of pancreatic cancer patients was analyzed. After knockdown of POTEE or treatment of the GSK‐3β inhibitor, proliferative change in pancreatic cancer cells was assessed. Protein levels of vital genes in the PI3K/Akt/GSK‐3β/β‐catenin signaling influenced by POTEE were examined. POTEE was upregulated in pancreatic cancer samples. High level of POTEE indicated advanced tumor staging, large tumor size, and poor prognosis in pancreatic cancer patients. Knockdown of POTEE or treatment of Tideglusib remarkably attenuated proliferative ability in pancreatic cancer cells. Vital genes in the PI3K/Akt/GSK‐3β/β‐catenin signaling were downregulated by knockdown of POTEE. POTEE stimulates the proliferative ability in pancreatic cancer by activating the PI3K/Akt/GSK‐3β/β‐catenin signaling. High level of POTEE indicates advanced tumor staging, large tumor size and poor prognosis in pancreatic cancer patients.
Cyanidin‐3‐O‐glucoside attenuates endothelial cell dysfunction by modulatingmiR‐204‐5p/SIRT1‐mediated inflammation and apoptosis
Authors: Wang Zhihong , Zhang Min , Wang Zhong , Guo Zongjun , Wang Zongqiu , Chen Qun 
Published: June 2020

AbstractEndothelial cell (EC) dysfunction is a major symptom associated with the initiation of atherosclerosis (AS). Cyanidin‐3‐O‐glucoside (C3G) has the potentials to attenuate AS symptoms. In the current study, the mechanism driving the effects of C3G on AS rabbits and injured ECs were explored by focusing on the changes in miR‐204‐5p/SIRT1 axis. AS symptoms were induced in rabbits using high‐fatty diet (HFD) plus balloon catheter injured method and handled with C3G of two doses. Then the changes in artery wall structure, hemodynamics parameters, blood lipid level, systemic inflammation, and miR‐204‐5p/SIRT1 axis were detected. EC dysfunction was imitated by subjecting human umbilical vein endothelial cells (HUVECs) to TNF‐α, which was then handled with C3G. The changes in apoptosis, inflammation, and miR‐204‐5p/SIRT1 axis were detected. The results showed that the administrations of C3G improved artery wall structure and hemodynamics parameters, decreased blood lipid levels, and suppressed pro‐inflammatory cytokine production in HFD rabbits, which was associated with the down‐regulation of miR‐204‐5p and the up‐regulation of SIRT1. In in vitro assays, the treatments of C3G suppressed apoptosis, inhibited inflammation, down‐regulated miR‐204‐5p level, and induced SIRT1 level in HUVECs. The overexpression of miR‐204‐5p impaired the protective effects of C3G on the injured HUVECs by increasing cell apoptosis and inflammation. The findings outlined in the current study confirmed the protective effects of C3G on EC function, which was associated with the down‐regulation of miR‐204‐5p and the up‐regulation of SIRT1.
Demonstrating the Manufacture of Human CAR‐T Cells in an Automated Stirred‐Tank Bioreactor
Authors: Costariol Elena , Rotondi Marco C. , Amini Arman , Hewitt Christopher J. , Nienow Alvin W. , Heathman Thomas R. J. , Rafiq Qasim A. 
Published: June 2020

AbstractChimeric antigen receptor T‐cell (CAR‐T) therapies have proven clinical efficacy for the treatment of hematological malignancies. However, CAR‐T cell therapies are prohibitively expensive to manufacture. The authors demonstrate the manufacture of human CAR‐T cells from multiple donors in an automated stirred‐tank bioreactor. The authors successfully produced functional human CAR‐T cells from multiple donors under dynamic conditions in a stirred‐tank bioreactor, resulting in overall cell yields which were significantly better than in static T‐flask culture. At agitation speeds of 200 rpm and greater (up to 500 rpm), the CAR‐T cells are able to proliferate effectively, reaching viable cell densities of >5 × 106 cells ml‐1 over 7 days. This is comparable with current expansion systems and significantly better than static expansion platforms (T‐flasks and gas‐permeable culture bags). Importantly, engineered T‐cells post‐expansion retained expression of the CAR gene and retained their cytolytic function even when grown at the highest agitation intensity. This proves that power inputs used in this study do not affect cell efficacy to target and kill the leukemia cells. This is the first demonstration of human CAR‐T cell manufacture in stirred‐tank bioreactors and the findings present significant implications and opportunities for larger‐scale allogeneic CAR‐T production.
Interactive learning modules with 3D printed models improve student understanding of protein structure–function relationships
Authors: Howell Michelle E. , Booth Christine S. , Sikich Sharmin M. , Helikar Tomáš , van Dijk Karin , Roston Rebecca L. , Couch Brian A. 
Published: June 2020

AbstractEnsuring undergraduate students become proficient in relating protein structure to biological function has important implications. With current two‐dimensional (2D) methods of teaching, students frequently develop misconceptions, including that proteins contain a lot of empty space, that bond angles for different amino acids can rotate equally, and that product inhibition is equivalent to allostery. To help students translate 2D images to 3D molecules and assign biochemical meaning to physical structures, we designed three 3D learning modules consisting of interactive activities with 3D printed models for amino acids, proteins, and allosteric regulation with coordinating pre‐ and post‐assessments. Module implementation resulted in normalized learning gains on module‐based assessments of 30% compared to 17% in a no‐module course and normalized learning gains on a comprehensive assessment of 19% compared to 3% in a no‐module course. This suggests that interacting with these modules helps students develop an improved ability to visualize and retain molecular structure and function.
Decoration of electrical conductive polyurethane‐polyaniline/polyvinyl alcohol matrixes with mussel‐inspired polydopamine for bone tissue engineering
Authors: Ghorbani Farnaz , Ghalandari Behafarid , Khan Ab Lateef , Li Dejian , Zamanian Ali , Yu Baoqing 
Published: June 2020

AbstractElectrospinning is a versatile technology for the fabrication of nanofibrous matrixes to regenerate defects. This study aims to develop a functionalized and electroconductive polymeric matrix to improve rat bone marrow mesenchymal stem cell adhesion, proliferation, and differentiation. Herein, the influence of the chemical composition of the substrate on homogeneous modification of the surface with mussel‐inspired polydopamine (PDA) is focused. Accordingly, the deposition of PDA on the surface was proved by Fourier transform infrared spectroscopy. Morphologies of the scaffolds demonstrated homogeneous decoration of the polyvinyl alcohol (PVA)/polyurethane (PU)‐polyaniline (PANI) matrixes with PDA, while a lower density of mussel‐inspired polymer was observed in bare PU‐PANI constructs. Although uniform and dense precipitation of PDA reduced conductivity of scaffolds 1.2 times compared with the samples with a low density of the PDA, 1.1 and 1.2 times enhancement in tensile strength and Young's modulus, respectively, were the strength of the applied process, especially in bone tissue engineering area. Contact angle measurements demonstrated about two times reduction in measured values, which shows improvement in hydrophilicity of PDA‐modified PVA/PU‐PANI fibers compared with PDA‐coated PU‐PANI ones. Swelling ratio and mass loss ratio calculations revealed enhancement in measured values as a function of homogeneous and dense coating, which arise from hydrophilicity of the polymeric substrate. The bioactivity test indicated that a dense layer of PDA strongly supports formations of hydroxyapatite‐like crystals. Moreover, homogeneous decoration of conductive matrixes with PDA showed suitable cell viability, adhesion, and spreading while cell‐scaffolds interactions improved under electrical stimulation. Higher expression of alkaline phosphatase and secretion of Collagen I under the electrical field proved the applicability of modified electroconductive scaffolds for further preclinical and clinical studies to introduce as a reconstructive bone substitute.
Development and validation of a pretreatment nomogram to predict overall survival in gastric cancer
Authors: Bando Etsuro , Ji Xinge , Kattan Michael W. , Seo Ho Seok , Song Kyo Young , Park Cho‐Hyun , Bencivenga Maria , de Manzoni Giovanni , Terashima Masanori 
Published: June 2020

AbstractBackgroundPretreatment clinical staging is essential to select therapy. However, there have been no published pretreatment gastric cancer nomograms constructed using pretreatment clinical prognostic factors, including in nonresection patients. We aimed to develop a new pretreatment gastric cancer nomogram for individualized prediction of overall survival (OS).MethodsThe nomogram was developed using data of 5231 Japanese gastric cancer patients, and it was created with a Cox regression model. Fifteen clinical variables, which were obtained at pretreatment, were collected and registered. Data of two independent cohorts of patients from Seoul St. Mary's Hospital (1001 patients), and the University of Verona (389 patients) formed the external validation cohorts. The model was validated internally and externally using measures of discrimination (Harrell's C‐index), calibration, and decision curve analysis.ResultsThe developed nomogram showed good discrimination, with a C‐index of 0.855; that of the American Joint Committee on Cancer (AJCC) clinical stage was 0.819. In the external validation procedure, the C‐indexes were 0.856 (AJCC, 0.795) in the Seoul St. Mary's cohort and 0.714 (AJCC, 0.648) in the University of Verona cohort. The nomogram performed well in the calibration and decision curve analyses when applied to both the internal and external validation cohorts. A stage‐specific subset survival analysis of the three risk groups calculated using the nomogram also showed the superiority of nomogram‐prediction when compared to AJCC.ConclusionThis new pretreatment model accurately predicts OS in gastric cancer and can be used for patient counseling in clinical practice and stratification in clinical trials.
Development and validation of an immune‐related prognostic signature in lung adenocarcinoma
Authors: Sun Sijin , Guo Wei , Wang Zhen , Wang Xin , Zhang Guochao , Zhang Hao , Li Renda , Gao Yibo , Qiu Bin , Tan Fengwei , Gao Yushun , Xue Qi , Gao Shugeng , He Jie 
Published: June 2020

AbstractBackgroundLung adenocarcinomas (LUAD) is the most common histological subtype of lung cancers. Tumor immune microenvironment (TIME) is involved in tumorigeneses, progressions, and metastases. This study is aimed to develop a robust immune‐related signature of LUAD.MethodsA total of 1774 LUAD cases sourced from public databases were included in this study. Immune scores were calculated through ESTIMATE algorithm and weighted gene co‐expression network analysis (WGCNA) was applied to identify immune‐related genes. Stability selections and Lasso COX regressions were implemented to construct prognostic signatures. Validations and comparisons with other immune‐related signatures were conducted in independent Gene Expression Omnibus (GEO) cohorts. Abundant infiltrated immune cells and pathway enrichment analyses were carried out, respectively, through ImmuCellAI and gene set enrichment analysis (GSEA).ResultsIn Cancer Genome Atlas (TCGA) LUAD cohorts, immune scores of higher levels were significantly associated with better prognoses (P < .05). Yellow (n = 270) and Blue (n = 764) colored genes were selected as immune‐related genes, and after univariate Cox regression analysis (P < .005), a total of 133 genes were screened out for subsequent model constructions. A four‐gene signature (ARNTL2, ECT2, PPIA, and TUBA4A) named IPSLUAD was developed through stability selection and Lasso COX regression. It was suggested by multivariate and subgroup analyses that IPSLUAD was an independent prognostic factor. It was suggested by Kaplan‐Meier survival analysis that eight out of nine patients in high‐risk groups had significantly worse prognoses in validation data sets (P < .05). IPSLUAD outperformed other signatures in two independent cohorts.ConclusionsA robust immune‐related prognostic signature with great performances in multiple LUAD cohorts was developed in this study.
Client attendance measures in counselling psychology trainees
Authors: Meier Scott T. 
Published: June 2020

AbstractCounselling supervisors and educators have yet to reach a consensus about standard measures for evaluating the performance of graduate student therapists. Compared to self‐reports and rater judgements, client attendance records provide low‐inference behavioural data partially reflective of clients’ engagement with therapists. This naturalistic study evaluates client attendance data using archival records of 92 doctoral students in beginning and advanced counselling practica who produced data for 771 clients, 3,949 scheduled sessions and 3,186 attended sessions. Providing evidence of the sample representativeness for clinicians, results indicated that the mean number of sessions attended per client for student therapists (5.65) was very similar to that reported in the literature for outpatient therapists (<6). As expected because of structural differences in client assignment, beginning and advanced students evidenced statistically significant differences on attendance variables such as number of clients and scheduled sessions. In contrast, attendance ratios were equivalent across counsellor experience, suggesting that these individual difference variables could be useful for evaluation of therapist competencies related to client engagement.
Impact of lesion preparation strategies on outcomes of left main PCI: The EXCEL trial
Authors: Beohar Nirat , Chen Shmuel , Lembo Nicholas J. , Banning Adrian P. , Serruys Patrick W. , Leon Martin B. , Morice Marie‐Claude , Généreux Philippe , Kandzari David E. , Kappetein Arie Pieter , Sabik Joseph F. , Dressler Ovidiu , McAndrew Thomas , Zhang Zixuan , Stone Gregg W. 
Published: June 2020

AbstractObjectivesWe examined outcomes according to lesion preparation strategy (LPS) in patients with left main coronary artery (LMCA) percutaneous coronary intervention (PCI) in the EXCEL trial.BackgroundThe optimal LPS for LMCA PCI is unclear.MethodsWe categorized LPS hierarchically (high to low) as: (a) rotational atherectomy (RA); (b) cutting or scoring balloon (CSB); (c) balloon angioplasty (BAL); and d) direct stenting (DIR). The primary endpoint was 3‐year MACE; all‐cause death, stroke, or myocardial infarction.ResultsAmong 938 patients undergoing LMCA PCI, RA was performed in 6.0%, CSB 9.5%, BAL 71.3%, and DIR 13.2%. In patients treated with DIR, BAL, CSB, and RA, respectively, there was a progressive increase in SYNTAX score, LMCA complex bifurcation, trifurcation or calcification, number of stents, and total stent length. Any procedural complication occurred in 10.4% of cases overall, with the lowest rate in the DIR (7.4%) and highest in the RA group (16.1%) (ptrend = .22). There were no significant differences in the 3‐year rates of MACE (from RA to DIR: 17.9%, 20.2%, 14.5%, 14.7%; p = .50) or ischemia‐driven revascularization (from RA to DIR: 16.8%, 10.8%, 12.3%, 14.2%; p = .65). The adjusted 3‐year rates of MACE did not differ according to LPS.ConclusionsThe comparable 3‐year outcomes suggest that appropriate lesion preparation may be able to overcome the increased risks of complex LMCA lesion morphology.
The contribution of fiber components to water absorption of wheat grown in the UK
Authors: Lovegrove Alison , Wood Abigail J. , Hassall Kirsty L. , Howes Liz , Poole Mervin , Tosi Paola , Shewry Peter 
Published: June 2020

AbstractBackground and objectivesThe water absorption (WA) of white wheat flour is a major factor affecting processing quality, and millers, therefore, process their wheat to achieve the required level. Although it is likely that WA is determined by the amounts and compositions of three major grain components, starch, protein, and arabinoxylan, the contribution of the latter is not agreed and not recognized in the widely used Farrand equation.FindingsWe have measured a range of parameters related to fiber amount and composition and tested the ability of these to improve the prediction of WA using a modified Farrand equation. The addition of a range of single fiber traits improved the prediction of WA from a baseline of 82.98% to a maximum of 86.78%, but inclusion of all fiber traits as PCs resulted in a further improvement to 90%. Inclusion of the PCs also accounted for variation in WA between harvest years. The greatest improvement from inclusion of a single trait was observed with β‐glucan, the inclusion of arabinogalactan peptide (AGP) also resulted in improved prediction of WA.ConclusionsThe study shows that fiber components contribute to variation in WA, including differences between harvest years, but that β‐glucan and AGP have similar or greater impacts than AX.Significance and noveltyThe study dissects the contributions of AX amount and composition to WA and demonstrates a contribution of b‐glucan for the first time.
In vitro antioxidant and angiotensin I‐converting enzyme inhibitory properties of enzymatically hydrolyzed quinoa (Chenopodium quinoa) and kiwicha (Amaranthus caudatus) proteins
Authors: Chirinos Rosana , Pedreschi Romina , Velásquez‐Sánchez Margoth , Aguilar‐Galvez Ana , Campos David 
Published: June 2020

AbstractBackground and objectivesThis study investigated the in vitro antioxidant and angiotensin I‐converting enzyme (ACE‐I) inhibitory properties of quinoa (QPH) and kiwicha (KPH) protein hydrolysates.FindingsEnzymatic treatments with Neutrase for 120 min for quinoa and sequential Alcalase‐Neutrase hydrolysis for 240 min for kiwicha protein, both at 50°C, presented high antioxidant activities and ACE‐I inhibition (1.50 and 1.67 μmol TE/mg of protein and 89.2 and 72.8%, respectively) and the lowest IC50 values (0.08 and 0.29 mg/ml, respectively). After simulated gastrointestinal digestion (pepsin–pancreatin), both protein hydrolysates did not display significant changes in their antioxidant and ACE‐I inhibition properties.ConclusionsThe in vitro antioxidant and antihypertensive properties (ACE‐I inhibition) of QPH and KPH obtained via enzymatic hydrolysis using food‐grade commercial enzymes were demonstrated. In addition, tested in vitro bioactive properties did not change after simulated gastrointestinal digestion.Significance and noveltyThe results of this research might be used to obtain QPH and KPH with bioactive properties and/or as starting material for subsequent processes of separation and purification to obtain bioactive peptides.
Application of chemometrics to prediction of some wheat quality factors by near‐infrared spectroscopy
Authors: Williams Phil C. 
Published: June 2020

AbstractBackground and objectivesPhysical quality parameters of wheat include kernel texture and test weight, which affect classification, grading, and price. Physicochemical factors are associated with flour functionality. The objective of the work was to determine the effectiveness with which these factors could be predicted in early generations using near‐infrared spectroscopy (NIRS). Wheat breeders need to know how their new genetic lines carry these factors, which carry no identifiable absorbers in the NIR region, can be predicted in early generations, by experts in the use of NIRS and its associated chemometrics.FindingsWith the exception of protein content, for which absorbers are plentiful, and was included to verify the spectral quality of the sample sets, none of the strictly physical factors could be reliably predicted with the most widely used chemometric options in the hands of experts in their use. Random forest algorithms were capable of prediction of all physicochemical factors, except Farinograph development time, with a reasonable degree of reliability.ConclusionsReliable predictions of quality factors in wheat that can be predicted by NIRS with satisfactory reliability are limited to chemical and physicochemical factors for which absorbers exist in the NIR region.Significance and noveltyChemical, physicochemical, and physical factors can be predicted with acceptable reliability by NIRS using a computerized spectrophotometer in association with Random Forest software. Farinograph development time remains a challenge for NIRS application.
A case of drug‐induced Stevens‐Johnson syndrome‐like eruption predominating in mucosa: A case report
Authors: Ishiguro Akihiro , Shibata Tomoyuki , Yanagishita Takeshi , Takama Hiroyuki , Uchida Rimi , Ohshima Yuichiro , Watanabe Daisuke 
Published: June 2020

AbstractAtypical Stevens‐Johnson syndrome (SJS) is a variant of SJS with complete absence of or only few cutaneous manifestations. It usually affects children with Mycoplasma‐induced respiratory infection. It was unique because our case was induced by drug and occurred in an adult.