bluuebunny/crossref_metadata_2025_split huggingface dataset:
Silencing of functional p53 attenuates NAFLD by promoting HMGB1-related autophagy induction
Authors: Zhang Xuequn , Lin Yiming , Lin Sisi , Li Chunxiao , Gao Jianguo , Feng Zemin , Wang Jinghua , Zhang Jie , Zhang Hong , Zhang Yuwei , Chen Xueyang , Chen Shenghui , Xu Chengfu , Li Youming , Yu Chaohui , Zeng Hang 
Published: June 2020

Abstract
Background and aim
Nonalcoholic fatty liver disease (NAFLD) is a common chronic liver disease worldwide, but its pathogenesis remains imprecisely understood and requires further clarification. Recently, the tumor suppressor p53 has received growing attention for its role in metabolic diseases. In this study, we performed in vivo and in vitro experiments to identify the contribution of p53–autophagy regulation to NAFLD.

Methods
Livers from wild-type and p53 knockout mice as well as p53-functional HepG2 cells and p53-dysfunctional Huh7 cells were examined for autophagy status and HMGB1 translocation. In vivo and in vitro NAFLD models were established, and steatosis was detected. In the cell models, autophagy status and steatosis were examined by p53 and/or HMGB1 silencing.

Results
First, the silencing of p53 could induce autophagy both in vivo and in vitro. In addition, p53 knockout attenuated high-fat diet-induced NAFLD in mice. Similarly, knockdown of p53 could alleviate palmitate-induced lipid accumulation in cell models. Furthermore, high mobility group box 1 (HMGB1) was proven to contribute to the effect of silencing p53 on alleviating NAFLD in vitro as an autophagy regulator.

Conclusion
The anti-NAFLD effect of functional p53 silencing is associated with the HMGB1-mediated induction of autophagy.

Graphic abstract
ADMA mediates gastric cancer cell migration and invasion via Wnt/β-catenin signaling pathway
Authors: Guo Q. , Xu J. , Huang Z. , Yao Q. , Chen F. , Liu H. , Zhang Z. , Lin J. 
Published: June 2020

Abstract
Objective
To explore the role of ADMA in gastric cancer.

Methods
The specimens of 115 gastric cancer patients were analyzed by ELISA and survival analysis. Functional assays were used to assess the effects of ADMA on gastric cancer cells. Experiments were conducted to detect the signaling pathway induced by ADMA in GC.

Results
Gastric cancer patients with high ADMA levels had poor prognosis and low survival rate. Furthermore, high level of ADMA did not affect the proliferation while promoted the migration and invasion of gastric cancer cell. Moreover, ADMA enhanced the epithelial–mesenchymal transition (EMT). Importantly, ADMA positively regulated β-catenin expression in GC and promoted GC migration and invasion via Wnt/β-catenin pathway.

Conclusions
ADMA regulates gastric cancer cell migration and invasion via Wnt/β-catenin signaling pathway and which may be applied to clinical practice as a diagnostic and prognostic biomarker.
Relationship between landslide and morpho-structural analysis: a case study in Northeast of Morocco
Authors: Benzougagh Brahim , Meshram Sarita Gajbhiye , Baamar Brahim , Dridri Abdallah , Boudad Larbi , Sadkaoui Driss , Mimich Khalid 
Published: June 2020

AbstractThe study of landslides in the Inaouene watershed (northeastern Morocco) provides information on the relationships between landslides and morpho-structural analysis. Landslides affect part of the slopes and slopes of the valley. The configuration of this relationship is controlled by the combination of several predisposing factors. Two localities were studied, located in similar morpho-structural contexts, but characterized by a different lithology. The first determining factor was lithology, in particular the dominance of friable geological formations, especially marls in the locality of Chebabate and limestone soft formations in the locality of Tahla. The second factor was the tectonics, which is well individualized in the limestone of Tahla; in spite of friable lithology of the marls of Chebabate, one manages to disturb traces of the tectonics notably with the level of intercalation of the sandstone benches. Another factor that controls the dissection and the evolution of the landslides is the precipitation by the effect of abundance of the water of impregnation in the formations. The fieldwork is devoted to the measurement of the different faults encountered in the two localities to make the stereographic projection in order to elaborate canvas. The principal objective of this study is to find the relation between the evolution of the risks of erosion and landslide as well as the various factors, who control their spatiotemporal evolution. The results obtained will help managers and decision-makers in the development of watersheds in order to take the necessary steps to reduce the negative impact of this natural hazard on the environment, the population and their property.
Potential risk assessment and spatial distribution of elemental concentrations in sediment
Authors: Ediagbonya T. F. , Balogun O. T. 
Published: June 2020

AbstractSediments are carrier and a sink of elements in the hydrological cycle. Monitoring of contaminated soil and sediment with metallic elements is of interest due to their influence on groundwater, surface water, plants, animals and humans. In this study, different sediment samples from five various places were taken, and the elemental concentration along with some physiochemical parameters were determined. The elemental concentrations were determined with proton-induced X-ray emission, while the physiochemical parameters were determined using a conductivity meter, pH meter and thermometer. This study showed that silicon had highest mean concentration and lead had the lowest mean concentration. Cadmium had the highest contamination values in all the locations. Okitipupa had the highest pollution load index, and the lowest pollution index was recorded in Irele. The overall risk index (RI) in all the locations in the sediment indicated very high ecological risk index. Pollution load index (PLI) also categorized ecological risk which ranged from 8.812 to 28.42. Mean PLI value (17.7) recorded in this study was far higher than the threshold (< 1), indicating the presence of heavy pollutant levels. Such high-level PLI values signified danger and measures are needed in order to reduce the sources of pollutants in the sediment. From enrichment value and Igeo-accumulation values, Sn was severely polluted. Si, Zr, Ag and Cd were extremely polluted. The physiochemical parameters correlated with the elemental concentrations both negatively and positively.
Polymorphism of TLR2 in bank vole populations in North Eastern Poland is not associated with Borrelia afzelii infection prevalence
Authors: Tarnowska Ewa , Niedziałkowska Magdalena , Stojak Joanna , Jędrzejewska Bogumiła 
Published: June 2020

AbstractPolymorphism in innate immune genes in host populations can structure spatial variation in the prevalence of infectious diseases. In Europe,Borrelia afzeliiis an important tick-borne pathogen of small mammals including the bank voles (Myodes glareolus). The Toll-like receptor 2 (TLR2) is an innate immune receptor that is important for detectingBorrelia burgdorferisensulatopathogens. The TLR2 gene is polymorphic in bank vole populations and is classified into four distinct clusters: C1, C2, C3, and C4. The C2 and C4 clusters versus the C1 and C3 clusters are associated with lower versus higher infection prevalence, respectively. We detected three TLR2 clusters in 487 bank voles from 30 populations in NE Poland: 84.2% of the obtained sequences belonged to the C1 variant, 7.2% to C2, and 8.6% to C3. However, no clear spatial structure of TLR2 clusters among the populations was detected.B. afzeliiinfection prevalence across all studied individuals was 12.1% and varied from 0 to 37.5% among populations. There were no significant differences inB. afzeliiprevalence among voles carrying alleles of different TLR2 clusters, or between individuals belonging to two mtDNA lineages. Most infected individuals were adults, and males were infected more often than females. There was no significant relationship between the prevalence of TLR2 clusters in the vole populations and climatic and environmental factors within the study area. We therefore could not confirm an adaptive role of the TLR2 C2 alleles in reducingB. afzeliiinfection prevalence in bank voles.
Linkages Between Tropical Cyclones and Extreme Precipitation over China and the Role of ENSO
Authors: Wang Licheng , Yang Zhengnan , Gu Xihui , Li Jianfeng 
Published: June 2020

AbstractThis research investigated the linkages between tropical cyclones (TCs) and extreme precipitation, and their associations with El Niño-Southern Oscillation (ENSO) over China. The contribution of TC-induced to total extreme precipitation events along the southeast coast of China was higher than 50%, and the values gradually decreased as TCs moved inland. However, the precipitation extremes (magnitude and frequency) related to TCs did not show statistically significant changes over the most recent 57 years. The impacts of TCs on precipitation extremes are evidently modulated by the ENSO phases. We found less extreme precipitation linked with TCs in southeastern China during El Niño phase, because of the fewer TC tracks over this region and less TC genesis in the western North Pacific (WNP). The small TC track density over southeastern China is due to the prevalent westerly steering flow and abnormal integrated vapor transport from northern to southern China during El Niño years. Additionally, warmer sea surface temperature, more vigorous westerlies, larger vorticity in 250 hPa, and higher divergence in 850 hPa in an El Niño phase jointly displaced the mean genesis of the WNP TCs eastward and this led to fewer TCs passing through southeastern China.
SR4R: An Integrative SNP Resource for Genomic Breeding and Population Research in Rice
Authors: Yan Jun , Zou Dong , Li Chen , Zhang Zhang , Song Shuhui , Wang Xiangfeng 
Published: June 2020

Abstract
The information commons for rice (IC4R) database is a collection of 18 million single nucleotide polymorphisms (SNPs) identified by resequencing of 5152 rice accessions. Although IC4R offers ultra-high density rice variation map, these raw SNPs are not readily usable for the public. To satisfy different research utilizations of SNPs for population genetics, evolutionary analysis, association studies, and genomic breeding in rice, raw genotypic data of these 18 million SNPs were processed by unified bioinformatics pipelines. The outcomes were used to develop a daughter database of IC4R – SnpReady for Rice (SR4R). SR4R presents four reference SNP panels, including 2,097,405 hapmapSNPs after data filtration and genotype imputation, 156,502 tagSNPs selected from linkage disequilibrium-based redundancy removal, 1180 fixedSNPs selected from genes exhibiting selective sweep signatures, and 38 barcodeSNPs selected from DNA fingerprinting simulation. SR4R thus offers a highly efficient rice variation map that combines reduced SNP redundancy with extensive data describing the genetic diversity of rice populations. In addition, SR4R provides rice researchers with a web interface that enables them to browse all four SNP panels, use online toolkits, as well as retrieve the original data and scripts for a variety of population genetics analyses on local computers. SR4R is freely available to academic users at http://sr4r.ic4r.org/.
Predictors of success in hypoglossal nerve stimulator implantation for obstructive sleep apnea
Authors: Chao Tiffany N. , Thaler Erica R. 
Published: June 2020

ObjectiveCurrent guidelines for hypoglossal nerve stimulator (HGNS) implantation eligibility include drug‐induced sleep endoscopy (DISE) findings and other patient characteristics but lead to highly variable rates of surgical success across institutions. Our objective was to determine whether additional factors seen on preoperative evaluation could be used as predictors of surgical success.Study designRetrospective chart review.SettingSingle‐institution academic tertiary care medical center.Subjectsand Methods:This study included patients with obstructive sleep apnea (OSA) who underwent HGNS implantation between 2015 and 2018. Surgical success was defined as a postoperative apnea‐hypopnea index (AHI) of less than 20 events per hour and an AHI reduction of at least 50%. Preoperative polysomnogram (PSG) results, DISE findings, and physical parameters were compared between surgical successes and failures.ResultsA total of 68 patients were included in the analysis. The overall surgical success rate was 79.4% (54/68). Elevated preoperative AHI was associated with an increased likelihood of treatment failure, with an AHI of (36.9 ± 16.8) events/hour in the success group compared to (49.4 ± 19.6) events/hour in the failure group (P = 0.05). Patients observed to have partial lateral oropharyngeal collapse on DISE was more frequently associated with the treatment failure group than in the success group (P = 0.04).ConclusionPatients who underwent HGNS implantation overall had a very high treatment response rate at our institution. Factors that may predispose patients to surgical failure included the presence of lateral oropharyngeal collapse and a significantly elevated preoperative AHI. These should be considered when determining surgical candidacy for HGNS implantation.
Demonstration of a prototype design synthesis capability for space access vehicle design
Authors: Rana L. , Chudoba B. 
Published: June 2020

ABSTRACTThe early conceptual design (CD) phase of space access vehicles (SAVs) is the most abstract, innovative and technologically challenging phase of the entire aerospace design life cycle. Although the design decision-making during this phase influences around 80 percent of the overall life cycle cost, it is the most abstract and thus least understood phase of the entire design life cycle. The history of SAV design provides numerous examples of project failures that could have been avoided if the decision-maker had had the capability to forecast the potential risks and threats correctly ahead of time during the conceptual design phase. The present study addresses this crucial phase and demonstrates a best-practice synthesis methodology prototype to advance the current state of the art of CD as applied to SAV design. Developed by the Aerospace Vehicle Design (AVD) Laboratory at the University of Texas at Arlington (UTA), the Aerospace Vehicle Design Synthesis process and software (AVDS) is a prototype solution for a flight vehicle configuration–flexible (generic) design synthesis capability that can be applied to the primary categories of SAVs. This study focusses on introducing AVDS, followed by the demonstration and verification of the system’s capability through a sizing case study based on the data-rich Boeing X-20 Dyna-Soar spaceplane.
Interleukin-6 and total antioxidant capacity levels following N-acetylcysteine and a combination nutraceutical intervention in a randomised controlled trial for bipolar disorder
Authors: Bortolasci C.C. , Voigt C. , Turner A. , Mohebbi M. , Gray L. , Dodd S. , Walder K. , Berk M. , Cotton S.M. , Malhi G.S. , Ng C.H. , Dowling N. , Sarris J. , Dean O.M. 
Published: June 2020

AbstractObjective:The aims of this study were to evaluate changes in inflammatory and oxidative stress levels following treatment with N-acetylcysteine (NAC) or mitochondrial-enhancing agents (CT), and to assess the how these changes may predict and/or moderate clinical outcomes primarily the Montgomery-Åsberg Depression Rating Scale (MADRS).Methods:This study involved secondary analysis of a placebo-controlled randomised trial (n = 163). Serum samples were collected at baseline and week 16 of the clinical trial to determine changes in Interleukin-6 (IL-6) and total antioxidant capacity (TAC) following adjunctive CT and/or NAC treatment, and to explore the predictability of the outcome or moderator effects of these markers.Results:In the NAC-treated group, no difference was observed in serum IL-6 and TAC levels after 16 weeks of treatment with NAC or CT. However, results from a moderator analysis showed that in the CT group, lower IL-6 levels at baseline was a significant moderator of MADRS χ2 (df) = 4.90, p = 0.027) and Clinical Global Impression-Improvement (CGI-I, χ2 (df) = 6.28 p = 0.012). In addition, IL-6 was a non-specific but significant predictor of functioning (based on the Social and Occupational Functioning Assessment Scale (SOFAS)), indicating that individuals with higher IL-6 levels at baseline had a greater improvement on SOFAS regardless of their treatment (p = 0.023).Conclusion:Participants with lower IL-6 levels at baseline had a better response to the adjunctive treatment with the mitochondrial-enhancing agents in terms of improvements in MADRS and CGI-I outcomes.
Royal Free Hospital-Nutritional Prioritizing Tool improves the prediction of malnutrition risk outcomes in liver cirrhosis patients compared with Nutritional Risk Screening 2002
Authors: Wu Yuchao , Zhu Yage , Feng Yali , Wang Ruojing , Yao Naijuan , Zhang Mengmeng , Liu Xiaohui , Liu Huan , Shi Lei , Zhu Li , Yang Nan , Chen Hongmei , Liu Jinfeng , Zhao Yingren , Yang Yuan 
Published: June 2020

AbstractThe European Society for Clinical Nutrition and Metabolism (ESPEN) guidelines recommend the Royal Free Hospital-Nutritional Prioritizing Tool (RFH-NPT) to identify malnutrition risk in patients with liver disease. However, little is known about the application of the RFH-NPT to screen for the risk of malnutrition in China, where patients primarily suffer from hepatitis virus-related cirrhosis. A total of 155 cirrhosis patients without liver cancer or uncontrolled co-morbid illness were enrolled in this prospective study. We administered the Nutritional Risk Screening 2002 (NRS-2002), RFH-NPT, Malnutrition Universal Screening Tool (MUST) and Liver Disease Undernutrition Screening Tool (LDUST) to the patients within 24 h after admission and performed follow-up observations for 1·5 years. The RFH-NPT and NRS-2002 had higher sensitivities (64·8 and 52·4 %) and specificities (60 and 70 %) than the other tools with regard to screening for malnutrition risk in cirrhotic patients. The prevalence of nutritional risk was higher under the use of the RFH-NPT against the NRS-2002 (63 v. 51 %). The RFH-NPT tended more easily to detect malnutrition risk in patients with advanced Child–Pugh classes (B and C) and lower Model for End-stage Liver Disease scores (<15) compared with NRS-2002. RFH-NPT score was an independent predictive factor for mortality. Patients identified as being at high malnutrition risk with the RFH-NPT had a higher mortality rate than those at low risk; the same result was not obtained with the NRS-2002. Therefore, we suggest that using the RFH-NPT improves the ability of clinicians to predict malnutrition risk in patients with cirrhosis primarily caused by hepatitis virus infection at an earlier stage.
Diet and sedentary behaviour in relation to mortality in US adults with a cardiovascular condition: results from the National Health and Nutrition Examination Survey linked to the US mortality registry
Authors: Ricci Cristian , Leitzmann Michael F. , Freisling Heinz , Schutte Aletta E. , Schutte Rudolph , Kruger Salome H. , Smuts Cornelius M. , Pieters Marlien 
Published: June 2020

AbstractCVD is the most common chronic condition and the highest cause of mortality in the USA. The aim of the present work was to investigate diet and sedentary behaviour in relation to mortality in US CVD survivors. The National Health and Nutrition Examination Surveys conducted between 1999 and 2014 linked to the US mortality registry updated to 2015 were investigated. Multivariate adjusted Cox regression was used to derive mortality hazards in relation to sedentary behaviour and nutrient intake. A multiplicative and additive interaction analysis was conducted to evaluate how sedentariness and diet influence mortality in US CVD survivors. A sample of 2473 participants followed for a median period of 5·6 years resulted in 761 deaths, and 199 deaths were due to CVD. A monotone increasing relationship between time spent in sedentary activities and mortality risk was observed for all-cause and CVD mortality (hazard ratio (HR) = 1·20, 95 % CI 1·09, 1·31 and HR = 1·19, 95 % CI 1·00, 1·67, respectively). Inverse mortality risks in the range of 22–34 % were observed when comparing the highest with the lowest tertile of dietary fibre, vitamin A, carotene, riboflavin and vitamin C. Sedentariness below 360 min/d and dietary fibre and vitamin intake above the median interact on an additive scale influencing positively all-cause and CVD mortality risk. Reduced sedentariness in combination with a varied diet rich in dietary fibre and vitamins appears to be a useful strategy to reduce all-cause and CVD mortality in US CVD survivors.
Effects of dietary carbohydrate sources on lipid metabolism and SUMOylation modification in the liver tissues of yellow catfish
Authors: Yang Shui-Bo , Zhao Tao , Wu Li-Xiang , Xu Yi-Chuang , Tan Xiao-Ying 
Published: June 2020

AbstractDysregulation in hepatic lipid synthesis by excess dietary carbohydrate intake is often relevant with the occurrence of fatty liver; therefore, the thorough understanding of the regulation of lipid deposition and metabolism seems crucial to search for potential regulatory targets. In the present study, we examined TAG accumulation, lipid metabolism-related gene expression, the enzyme activities of lipogenesis-related enzymes, the protein levels of transcription factors or genes involving lipogenesis in the livers of yellow catfish fed five dietary carbohydrate sources, such as glucose, maize starch, sucrose, potato starch and dextrin, respectively. Generally speaking, compared with other carbohydrate sources, dietary glucose promoted TAG accumulation, up-regulated lipogenic enzyme activities and gene expressions, and down-regulated mRNA expression of genes involved in lipolysis and small ubiquitin-related modifier (SUMO) modification pathways. Further studies found that sterol regulatory element binding protein 1 (SREBP1), a key transcriptional factor relevant to lipogenic regulation, was modified by SUMO1. Mutational analyses found two important sites for SUMOylation modification (K254R and K264R) in SREBP1. Mutant SREBP lacking lysine 264 up-regulated the transactivation capacity on an SREBP-responsive promoter. Glucose reduced the SUMOylation level of SREBP1 and promoted the protein expression of SREBP1 and its target gene stearoyl-CoA desaturase 1 (SCD1), indicating that SUMOylation of SREBP1 mediated glucose-induced hepatic lipid metabolism. Our study elucidated the molecular mechanism of dietary glucose increasing hepatic lipid deposition and found that the SREBP-dependent transactivation was regulated by SUMO1 modification, which served as a new target for the transcriptional programmes governing lipid metabolism.