bluuebunny/crossref_metadata_2025_split huggingface dataset:
Non-drug hemp, as a new variety of Cannabis sativa L. convar. non-narcotic Vyrovets
Authors: Vyrovets V. H. , Layko I. M. , Horshkova L. M. , Mishchenko S. V. , Kirichenko G. I. , Biryukova T. S. , Layko H. M. 
Published: November 2019

Aim. In the second half of the 20th and the beginning of the 21st century, there were significant changes in the hemp growing that affected the improvement of morphological, biological and economic properties, by providing the opportunity, on the example of new varieties, to declare the creation of a new subset of sown hemp in which the main achievements of breeders and seedlings were accumulated. Methods. With the creation of the Institute of Bast Crops, scientific breeding has begun, based on improved and developed new breeding methods that include several significant steps as increasing of the yield of created monoecious form, as the elimination of their narcotic properties. Results. For the first time in the world, non-drug hemp that can produce high yields of straw, fiber and seeds while maintaining immunity to damage to pests and diseases were created. Conclusions. Responding to the doubts of tax of collectors and botanists about the classification of the hemp family, we share the opinion of the first scientists and our teachers that include Cannabis sativa L. and Cannabis indica L. to one species of Cannabis sativa L., combining all kinds and varieties. The elimination of a number of high-yielding varieties of non-drug monoecious hemp, which became the result of long-term breeding, testifies the creation of a new variety of Cannabis sativa L. convar non-narcotic Vyrovets, emphasizing the leadership and executive role of Professor V. Vyrovets during a long selection process.
Keywords: hemp, genus, variety, geographical types, selection, productivity, elimination of narcotic.
The effect of nanoparticles of biogenic metal oxides on seed germination capability, photosynthetic pigment content, activities of PS II and superoxide dismutase in wheat seedlings
Authors: Gasimova F. I. , Khanishova M. A. , Taghiyeva K. R. , Azizov I. V. 
Published: November 2019

Aim. The effect of nanoparticles of copper, titanium, iron and aluminum oxides on the germination energy, germination capability, growth and development, the content of photosynthetic pigments, the activity of photosystems and superoxide dismutase of wheat seedlings was studied. Methods. The objects of the research were bread wheat (Triticum aestivum L) seedlings. The seeds of the experimental plants were treated with powders of nanoparticles of СuO, Fe2O3, ZnO, Al2O3, and TiO2, then sown in Petri dishes and in the pots with soil. Results. Nanoparticles were found to express various effects: СuO, and Al2O3 decreased the growth of seedlings, whereas TiO2, ZnO and Fe2O3 accelerating the growth of seedlings had a positive effect on the activities of PSII and superoxide dismutase. Conclusions. Nanoparticles of TiO2, ZnO and Fe2O3 had a positive effect on seed germination and accelerated the growth of wheat seedlings. Nanoparticles of ZnO and Fe2O3 also positively influenced on the activity of PS II, and the activity of the antioxidant enzyme-superoxide dismutase also increased.
Keywords: wheat, nanoparticles, germination, growth of seedlings, PS II, superoxide dismutase.
Usage of microsatellite loci linked with E genes, for identification and certification of soybean varieties
Authors: Zharikova D. O. , Aksyonova E. O. , Chebotar G. O. , Chebotar S. V. 
Published: November 2019

Aim. To investigate the molecular genetic polymorphism of soybean varieties created in different breeding centers of Ukraine by the loci Satt100, Satt229, Satt319, Satt354, Satt365, Sat_038 and to determine whether differentiation, identification and certification of varieties based on MS-analysis on listed loci can be used. Methods. DNA extraction, SSR-PCR, electrophoresis in 7% polyacrylamide gels and on ABI PRISM® Genetic Analyzer 3500 (Applied Biosystems), cluster analysis UPGMA. Results. In general, 28 alleles were detected. The most polymorphic locus was Satt365 − 6 alleles per locus. We did not detect heterozygous plants and heterogeneous soy varieties for studied loci. Conclusions. All varieties have been clearly differentiated on the dendrogram, that allows to offer an individual genetic formula for each of the varieties and recommend these microsatellite markers for use in the certification and registration of soy varieties.
Keywords: soybean, molecular genetic polymorphism, microsatellite loci.
Polymorphism of storage proteins of common backwheat (Fagopyrum esculentum Moench.) in groups of varieties with different flower color
Authors: Zaika Y. V. , Kozub N. A. , Sozinov I. A. , Bidnyk G. Ya. , Karazhbey P. P. 
Published: November 2019

Aim. To establish the presence of polymorphism and differences in variants of the protein spectrum of buckwheat seed storage proteins in groups of varieties with different colors of the flower corolla. Methods. Electrophoresis using the Laemmli method in 17.5% of the separating PAAG gels, visualization and identification of the spectrum variants. Results. The common variants of the electrophoretic spectrum for all groups of buckwheat varieties and the heterogeneity of each of the studied groups of genotypes are revealed. By frequency of variants of electrophoretic spectrum between groups of varieties were detected statistically significant differences and reliable predominance of individual variants in green-flower morphotypes of buckwheat were established. Conclusions. Differences in frequency of occurrence of variants of electrophoretic spectrum in different morphotypes of buckwheat indicate the probable processes of preservation of individual adaptive alleles of genes of seed storage proteins, which occurred in populations when varieties under the pressure of breeding process.
Keywords: edible buckwheat, spare proteins, globulins, albumins, polymorphism.
Physiological and biochemical analysis of transgenic wheat plants of seed generation T2 with double-stranded RNA suppressor of the proline dehydrogenase gene
Authors: Kulesh S. S. , Dubrovna O. V. , Slivka L. V. 
Published: November 2019

Aim. To carry out physiological and biochemical analysis of genetically modified plants of bread wheat of seed generation T2 with the double-stranded RNA suppressor of the proline dehydrogenase gene. Methods. Biochemical determination of free L-proline content and activity of the enzyme proline dehydrogenase; physiological examination of plant growth in vitro and in vivo. Results. It has been shown that transgenic plants, in contrast to control groups, grow more intensively in a selective medium with mannitol, maintaining a green color. It has been established that under normal conditions and under conditions of water deficit, plants of the T2 have an elevated level of free proline in the leaves, compared with the control genotypes. It was found that transformants are characterized by reduced activity of the enzyme of proline dehydrogenase, which manifests itself when the norm-stress-norm conditions change. Transgenic T2 plants had a higher resistance to aqueous deficiency compared to baseline, which was reflected in the nature of their growth. In conditions of soil moisture shortage, the yield of the most of the transformed lines was significantly higher than non-transformed plants. Conclusions. The obtained results allow us to conclude that the use of the pBi2E vector construct with the double-stranded RNA suppressor of the pdh gene is effective for the production of transgenic bread wheat plants with a high level of resistance to water deficiency.
Keywords: Triticum aestivum L., Agrobacterium-mediated transformation, RNA suppressor of the proline dehydrogenase gene, T2 plants, physiological-biochemical analysis.
Induction of breeding-valuable mutations in Triticum aestivum L. under the influence of physical and chemical mutagenic factors of the environment
Authors: Morgun V. V. , Yakymchuk R. A. 
Published: November 2019

Aim. The use of induced mutagenesis opens greater possibilities to drastically improve cultivated plants genetically. The areas of local and mass anthropogenic pollution have unique conditions of the effect of mutagenic factors, and they require the studying of their possible use while developing parental material for winter wheat breeding. The induction frequency of breeding-valuable mutations in Triticum aestivum L., affected by the contamination with physical and chemical mutagenic factors of the environment, was studied. Methods. The choice of mutants was made in М2 and М3 generations, using record techniques and separating visible mutations, phonological observations, biometric indicator analysis. Results. The pollutants of the environment cause the frequency increase of breeding-valuable mutations in T. aestivum L. by 7.5–12.5 times under the effect of the contamination with natural radioisotopes of the area of uranium ore mining, by 2.1–19.7 times – with heavy metals of industrial enterprises, and 4.1–9.8 times – with pesticides, prohibited and not suitable for use, and toxic wastes in the places of their storage. Spectrum is predominantly represented with low-grown forms, forms with intensive growth, a long-cylindrical spike, and it depends on the nature of a polluting agent and a genotype of a plant. Conclusions. The increase of frequency and the broadening of spectrum of breeding-valuable mutations, due to the induced mutagenesis with the environmental pollution, make it possible to use it for the implementation of breeding-genetic programs aimed at improving wheat cultivars.
Keywords: Triticum aestivum L., mutagenic factors, mutative variability, breeding-valuable mutations.
Application of mycorrhizal fungus Tuber melanosporum to stimulate the growth and development of soybean and spring barley
Authors: Oliferchuk V. P. , Fedorovych D. V. 
Published: November 2019

Aim. To evaluate the effect of soybean and spring barley seeds treatment with haploid cells of fungus Tuber melanosporum IMB F- 100106 on the growth and development of soybean and spring barley. Methods. The study was conducted in field conditions on the experimental field of the Department of Agroecology and Biosafety IAP NANU. The level of development of the fungus was evaluated visually and by counting ectomycorrhizal threads. Morphometric and biochemical methods were used to characterize the structure of the crop. Results. The treatment of soybean and spring barley seeds with T. melanosporum promotes productive growth and increases the yield of crops. Conclusions. Plant inoculation with T. melanosporum promotes productive growth and increases the yield of soybean and barley, promotes the possibility of creating new technologies of organic and regenerative agriculture and allows preserving of the genetic stability of this fungus. Keywords: mycorrhiza, Tuber melanosporum, crop yields, genetic stability.
Assessment of functional relationships between acids and neutral compounds of canabinoids in modern monoecious varieties of Cannabis sativa L.
Authors: Polyakova A. S. 
Published: November 2019

Aim. The research was aimed at determining the correlation between neutral substances and acids in varieties of Cannabis sativa L. Methods. Taking into account lability and isomerization of cannabinoids under the influence of various factors, the TLC method was used for preserving the naturally formed ratio of cannabinoids without disturbing their qualitative and quantitative interrelations. Results. In the hemp variety YuSO 1 excluded from breeding work to reduce the content of neutral substances, varieties of French selection – Fedora 17 and Futura 77 with relatively high content of cannabinoids and with varieties of hemp Zolotonis’ky 15 and Viktoriya with reduced or absent content of neutral substances, high interdependence between neutral compounds and acids was determined. In the varieties of hemp YuSO 31, Hlyana, Hlukhivs’ky 46 insufficient selections for reducing the content of neutral substances led to a violation of the formed relationships between acids and neutral substances. Correlation interdependence was negative. Conclusions. The determined correlation between neutral substances and acids in cannabis varieties indicates the existing biologically determined dependence between them taking into account natural acids.
Keywords: THC, CBD, CBN, CBDА, THCA.
Isolation and identification of a strain producing riboflavin
Authors: Radchenko M. M. , Beyko N. E. , Andriiash G. S. , Тigunova О. О. , Shulga С. М. 
Published: November 2019

Aim. Aim of investigation was to receive riboflavin strain-producers using natural sources for development of riboflavin technology. Methods. Strain-producers were isolated by the method of imprints (replica). The identification of stains was done by commonly used techniques using the «Bergey's Manual of Systematic Bacteriology». The resulting clones were tested for accumulation of riboflavin by fluorometric method. Results. 9 natural sources (seeds of corn and potato tubers) were investigated, pure cultures of microorganisms werr isolated and their identification was carried out. Two types of bacterial colonies of the genus Bacillus were identified. Selected strains weretested for antibiotic susceptibility and for the ability to accumulate riboflavin. Conclusions. As a result of the research, strain-producing riboflavin is isolated, the strain is classified as B. subtilis. The strain accumulated 4.3 g / l of riboflavin in a sucrose medium during a 72 hours cultivation. This strain was accepted as a source for the development of riboflavin technology.
Keywords: riboflavin, stain, microbial synthesis, Bacillus subtilis.
Related to Ppd-1 and Vrd gene systems peculiarities of initial development rate in new European winter bread wheat cultivars
Authors: Stelmakh A. F. , Fayt V. I. 
Published: November 2019

Aim. Vernalization response and photosensitivity were evaluated in some new cultivars originating from West European countries. Methods. Vrd and Ppd-1gene effects were measured by comparing numbers of days to heading at planting in natural and 10-hours photoperiods after preliminary green seedling vernalization of various duration. Those genes inheritance was studied under environment combinations securing the division of their effects. Results. Studied gene effects were inherited as partially dominant with additive-epistatic interactions. Evaluated stocks characterized often by durable vernalization requirement and photosensitivity presence differing essentially from modern Ukrainian cultivars. Conclusions. We call in question the assertion of leading Ukrainian breeders that strong physiological reactions of initial development delay are the factors limiting the modern productivity level in winter bread wheat.
Keywords: winter bread wheat, heading dates, vernalization requirement, photosensitivity, modern cultivars, productivity.
The role of allelic and nonallelic interactions of genes in the mechanism of heterosis
Authors: Hablak S. G. , Abdullaeva Ya. A. , Ryabovol L. O. , Ryabovol Ya. S. 
Published: November 2019

Aim. Studying the heterosis effect from the point of view of allelic and nonallelic gene interaction in F1 hybrids from crossing the arabidopsis ecotypes Col-0 and La-0. Methods. Hybridological method of crossing mutant lines and analysis of the inheritance of the signs of the root system. Results. When plants of different races Col-0 and La-0 cross in the F2 generation, polymeric interaction of genes occurs. In that case splitting in F2 goes in the ratio 15: 1. At the same time, hybrids of the first generation exhibit heterosis, which is manifested in a more powerful development of a rosette of leaves in comparison with the initial forms. Conclusions. The emergence of heterosis in hybrids of the first generation can be explained on the basis of the allelic and nonallelic gene interaction, which creates a favorable combination of genes during hybridization, causing the best manifestation of an economically valuable trait.
Keywords: Arabidopsis thaliana (L.) Heynh., heterosis, gene, mutation, race.
Features of the chromosomal apparatus in patients with juvenile idiopathic arthritis with concomitant multifactorial pathology
Authors: Bagatska N. V. , Nefidova V. E. , Medzianovska O. V. 
Published: November 2019

Aim. To determine the level of chromosomal disorders in peripheral blood lymphocytes of children and adolescents with juvenile idiopathic arthritis with concomitant multifactorial pathology in vitrо. Methods. Cytogenetic analysis was carried out in patients with juvenile idiopathic arthritis with concomitant multifactorial pathology in vitro, using a common methodology. Statistical analysis of the results of research was carried out using Excel software package and SPSS Statistics 17.0. Results. Chromosomal aberrations in peripheral blood lymphocytes were revealed in 100 % of patients. The general level of chromosomal abnormalities in the patients with concomitant pathology was 5.57 per 100 cells, and in the comparison group – 4.10 per 100 cells. In both groups aberrations of chromatid type prevailed. Conclusions. In patients with juvenile idiopathic arthritis, accompanied by different multifactorial disorders, spontaneous level of chromosomal abnormalities in blood lymphocytes in vitro was 1.4 times higher compared to the level of chromosomal aberrations in patients without concomitant pathology. The frequency of chromatid type aberrations was higher than the chromosome type aberrations rate in 1.6 times in the main group and in 2.2 times in the comparison group.
Keywords: juvenile idiopathic arthritis, children, adolescents, chromosomes, aberrations, concomitant multifactorial pathology.
Telomere lengths in women with early reproductive losses
Authors: Huleyuk N. I. , Zastavna D. V. , Tyrka M. 
Published: November 2019

Aim. Study of the relative telomere lengths in women with a history of early reproductive losses. Methods. Relative Telomere Length (RTL) was studied in the peripheral blood lymphocytes using a real time polymerase chain reaction (RT-PCR). Results. RTL was studied in 281 women, among which 169 - with early reproductive losses (ERL) (surveyed group) and 112 - have healthy children and no reproductive losses in history (control group). For women under the age of 35, the average RTL value is significantly higher than that of older women, P = 0.003597. In women with ERL, RTL is significantly lower than that of women with a preserved reproductive function, P = 0.0000001. The value of RTL is significantly lower in women with ERL under 35 years compared with control, P = 0.0000001, and is similar to value of RTL in women in the control group at the age of 36, P> 0.05. Conclusions. The telomere lengths is significantly lower in both older women and women with ERL. The similarity of RTLvalue in women with ERL up to 35 years of age and in women with a preserved reproductive function at the age of 36 years testifies to the telomeric theory of reproductive aging. Keywords: telomeres, RT-PCR, women, age, early reproductive loss.
Stem cells and genetics of development
Authors: Lukash L. L. 
Published: November 2019

Such issues of developmental genetics as preformism and epigenesis, potential totipotency of somatic cells of an organism, the role of stem cells (SCs) in differentiation, tissue formation and regeneration, homeotic genes that control these processes were reviewed. Signal regulatory systems (Wnt-signaling and others) take part in the realization of potential ability of stem and differentiated cells of an organism to reversible transmission paths or reprogramming from one stations to the others. The universal properties of the SCs (the ability to the selfrenewing by divisions, differentiation, transdifferentiation, etc.) were formed on the basis of homologous genes of the simple organisms in the course of a prolonged evolution of living forms. Structural, functional and regulatory systems of the simple and complex eukaryotic organisms have much in common, and, probably, their individual elements might be interchangeable. The implementation of these functions is based on the use of common signal regulatory systems which control the universal functions of the cell. A specific example of the regulation of myogenic differentiation during embryogenesis and tissue regeneration is considered.
Keywords: developmental genetics, preformism, epigenesis, somatic cell totipotency, stem cells (SC), differentiation, reprogramming, embryogenesis, regeneration, homeotic genes, myogenesis.
Genetic and epigenetic alterations of VHL gene in clear cell renal cell carcinoma
Authors: Onyshchenko K. V. , Grygorenko V. M. , Pereta L. V. , Serbai Yu. R. , Voitsitskyi T. V. , Skrypkina I. Ya. 
Published: November 2019

Aim. Renal cell carcinomas (RCC) – cancerous neoplasms of the genitourinary system representing about 3% of human malignant tumors. For malignancy degree indexing and tumor typing, shape of cell nucleus is widely used. However, genetic changes, in particular inactivation of von Hippel-Lindau (VHL) gene can serve as indicators of RCC progression. Thus, the purpose of our study was establishing the methylation status and loss of heterozygosity of the VHL gene as a potential and applicable clinical marker of kidney tumors. Methods. Determination of allelic imbalance in VHL gene expression was performed by PCR of STR-markers with subsequent fragments separation in 8% PAAG and by capillary gel electrophoresis of fluorescent-labeled PCR fragments. Methyl-specific PCR was used for epigenetic variability of VHL gene promoter. To detect statistically significant differences between tumor specimens and adjacent kidney tissues, Fisher's exact test and Mann-Whitney U-criterion were applied. Results. In 57% of the tumor samples for the marker D3S1038 and 48% for the D3S1317 loss of heterozygosity of the VHL gene was detected. Polymorphic information content for these loci was 84% for D3S1038 and 90% for D3S1317. The VHL promoter hypermethylation was 77%. Conclusions. The obtained results indicate that VHL gene can be reviewed as a candidate for not only diagnostic, but also prognostic application in RCC cancer.
Keywords: clear cell renal cell carcinoma, epigenetic changes, methylation, loss of heterozygosity, VHL.
Analysis of hsa-miR-30a-5p and hsa-miR-200c-3p microRNA expression in brain tumor
Authors: Skrypkina I. Ya. , Onyshchenko K. V. , Gerasymchuk D. О. , Anopriyenko O. V. , Areshkov P. O. 
Published: November 2019

Aim. Despite scientific and clinical success, glioblastoma remains the most aggressive tumor of the brain with extremely low prognostic potential for the patient. Biomarkers determining prognosis, and hence a treatment strategy, remains an actual problem at the moment. miRNAs can be considered as markers of glioblastoma. World’s long-term study confirm the legitimacy of miRNAs usage as reliable markers for the tumors diagnosis and prognosis, in particular brain tumors. The purpose of our study was miRNAs targeting, the change in expression of which in gliomas can be reliably associated with malignancy degree and tumor progression. Methods. To achieve the purpose of the investigation there were used such methods as bioinformatics search of the miRNAs for 3’-UTR genes associated with gliomas development, RNA isolation and miRNA-specific synthesis of cDNAs. The expression levels of miRNAs were determined by the real-time quantitative PCR (qPCR). The relative expression levels of targeted miRNAs were evaluated by comparing their content in tumor and adjacent tissues, conditionally healthy brain tissues of the same patients. Results. The analysis of qPCR results showed a decreased concentration of miR-30a-5p and miR-200c-3p in brain tumors relative to adjacent normal tissue in average of the 5 and 5.8 times, respectively (p <0.0001). Area under the ROC curve analysis of miR-30a-5p was AUC = 0.88528, for miR-200c-3p - AUC = 0.808. Conclusions. As an additional diagnostic and prognostic marker of glibalastoma signature determination, the expression level of hsa-miR-30a-3p and hsa-miR-200c-3p showed a good diagnostic potential (AUC = 7-9).
Keywords: glioblastoma, microRNA, hsa-miR-30a-5p, hsa-miR-200c-3p.