bluuebunny/crossref_metadata_2025_split huggingface dataset:
Integrative proteomics reveals principles of dynamic phospho-signaling networks in human erythropoiesis
Authors: Karayel Özge , Xu Peng , Bludau Isabell , Bhoopalan Senthil Velan , Yao Yu , Freitas Colaco Ana Rita , Delgado Alberto Santos , Schulman Brenda A. , Alpi Arno F. , Weiss Mitchell J. , Mann Matthias 
Published: May 2020

SUMMARYHuman erythropoiesis is exquisitely controlled at multiple levels and its dysregulation leads to numerous human diseases. Despite many functional studies focused on classical regulators, we lack a global, system-wide understanding of post-translational mechanisms coordinating erythroid maturation. Using the latest advances in mass spectrometry (MS)-based proteomics we comprehensively investigate the dynamics of protein and post-translational regulation ofin vitroreconstituted CD34+HSPC-derived erythropoiesis. This quantifies and dynamically tracks 7,400 proteins and 27,000 phosphorylation sites. Our data reveals differential temporal protein expression encompassing most protein classes and numerous post-translational regulatory cascades. Drastic cell surface remodeling across erythropoiesis include numerous orchestrated changes in solute carriers, providing new stage-specific markers. The dynamic phosphoproteomes combined with a kinome-targeting CRISPR/Cas9 screen reveal coordinated networks of erythropoietic kinases and downregulation of MAPK signaling subsequent to c-Kit attenuation as key drivers of maturation. Our global view of erythropoiesis establishes a central role of post-translational regulation in terminal differentiation.
Awakening a latent carbon fixation cycle inEscherichia coli
Authors: Satanowski Ari , Dronsella Beau , Noor Elad , Vögeli Bastian , He Hai , Wichmann Philipp , Erb Tobias J. , Lindner Steffen N. , Bar-Even Arren 
Published: May 2020

AbstractCarbon fixation is one of the most important biochemical processes. Most natural carbon fixation pathways are thought to have emerged from enzymes that originally performed other metabolic tasks. Can we recreate the emergence of a carbon fixation pathway in a heterotrophic host by recruiting only endogenous enzymes? In this study, we address this question by systematically analyzing possible carbon fixation pathways composed only ofEscherichia colinative enzymes. We identify the GED (Gnd-Entner-Doudoroff) cycle as the simplest pathway that can operate with high thermodynamic driving force. This autocatalytic route is based on reductive carboxylation of ribulose 5-phosphate (Ru5P) by 6-phosphogluconate dehydrogenase (Gnd), followed by reactions of the Entner-Doudoroff pathway, gluconeogenesis, and the pentose phosphate pathway. We demonstrate thein vivofeasibility of this new-to-nature pathway by constructingE. coligene deletion strains whose growth on pentose sugars depends on the GED shunt, a linear variant of the GED cycle which does not require the regeneration of Ru5P. Several metabolic adaptations, most importantly the increased production of NADPH, assist in establishing sufficiently high flux to sustain this growth. Our study exemplifies a trajectory for the emergence of carbon fixation in a heterotrophic organism and demonstrates a synthetic pathway of biotechnological interest.
Circadian clock mechanism driving mammalian photoperiodism
Authors: Wood S.H. , Hindle M.M. , Mizoro Y. , Cheng Y. , Saer B.R.C. , Miedzinska K. , Christian H.C. , Begley N. , McNeilly J. , McNeilly A.S. , Meddle S.L. , Burt D.W. , Loudon A.S.I. 
Published: May 2020

AbstractThe annual photoperiod cycle provides the critical environmental cue synchronizing rhythms of life in seasonal habitats. In 1936, Bünning proposed a circadian-based coincidence timer for photoperiodic synchronization in plants. Formal studies support the universality of this so-called coincidence timer, but we lack understanding of the mechanisms involved. Here we show in mammals that long photoperiods induce the circadian transcription factorBMAL2, in thepars tuberalisof the pituitary, and triggers summer biology through the eyes absent / thyrotrophin (EYA3 / TSH) pathway. Conversely, long-duration melatonin signals on short photoperiods induce circadian repressors includingDEC1, suppressing BMAL2 and the EYA3/TSH pathway, triggering winter biology. These actions are associated with progressive genome-wide changes in chromatin state, elaborating the effect of the circadian coincidence timer. Hence, circadian clock-pituitary epigenetic pathway interactions form the basis of the mammalian coincidence timer mechanism. Our results constitute a blueprint for circadian-based seasonal timekeeping in vertebrates.
Discrete regulation of β-catenin-mediated transcription governs identity of intestinal epithelial stem cells
Authors: Borrelli Costanza , Valenta Tomas , Handler Kristina , Vélez Karelia , Moro Giulia , Lafzi Atefeh , de Vargas Roditi Laura , Hausmann George , Moor Andreas E , Basler Konrad 
Published: May 2020

AbstractThe homeostasis of the gut epithelium relies upon continuous renewal and proliferation of crypt-resident intestinal epithelial stem cells (IESCs). Wnt/β-catenin signaling is required for IESC maintenance, however, it remains unclear how this pathway selectively governs the identity and proliferative decisions of IESCs. Here, we demonstrate that C-terminally-recruited transcriptional co-factors of β-catenin act as all-or-nothing regulators of Wnt-target gene expression. Blocking their interactions with β-catenin rapidly induces loss of IESCs and intestinal homeostasis. Conversely, N-terminally recruited co-factors fine-tune β-catenin’s transcriptional output to ensure proper self-renewal and proliferative behaviour of IESCs. Impairment of N-terminal interactions triggers transient hyperproliferation of IESCs, eventually resulting in exhaustion of the self-renewing stem cell pool. IESC mis-differentiation, accompanied by intrinsic and extrinsic stress signalling results in a process resembling aberrant “villisation” of intestinal crypts. Our data suggest that IESC-specific Wnt/β-catenin output requires discrete regulation of transcription by transcriptional co-factors.
Human iPSC-derived hypertrophic chondrocytes reveal a mutation-specific unfolded protein response in chondrodysplasias
Authors: Pretemer Yann , Kawai Shunsuke , Watanabe Makoto , Nagata Sanae , Nishio Megumi , Tamaki Sakura , Alev Cantas , Xue Jing-Yi , Wang Zheng , Fukiage Kenichi , Tsukanaka Masako , Futami Tohru , Ikegawa Shiro , Toguchida Junya 
Published: May 2020

SummaryChondrodysplasias are hereditary diseases caused by mutations in the components of growth cartilage. Although the unfolded protein response (UPR) has been identified as a key disease mechanism in mouse models, no suitablein vitrosystem has been reported to analyze the pathology in humans. Here, utilizing human chondrodysplasia-specific iPSCs, we examined the UPR caused by mutations inMATN3orCOL10A1. In growth plate-like structures formed from iPSC-derived sclerotomein vivo, the hypertrophic zone was disrupted, and induced hypertrophic chondrocytesin vitroshowed varying levels of ER stress depending on the mutation. Autophagy inducers and chemical chaperones succeeded in reducing ER stress only in some mutants, while transcriptome analysis revealed many mutation-specific changes in genes involved in apoptosis, metabolism, and protein trafficking. In this way, our system has allowed the precise evaluation of the UPR caused by each mutation, opening up new avenues for treatment of individual chondrodysplasia patients.
Paternal multigenerational exposure to an obesogenic diet drives epigenetic predisposition to metabolic disorders
Authors: Raad Georges , Serra Fabrizio , Martin Luc , Derieppe Marie-Alix , Gilleron Jérôme , Costa Vera L , Pisani Didier F , Amri Ez-Zoubir , Trabucchi Michele , Grandjean Valérie 
Published: May 2020

AbstractObesity is a growing societal scourge responsible for approximately 4 million deaths worldwide. Recent studies have uncovered that paternal excessive weight induced by an unbalanced diet affects the metabolic health of offspring. These reports mainly employed single-generation male exposure. However, the consequences of multigenerational unbalanced diet feeding on the metabolic health of progeny remain largely unknown. Here, we show that maintaining paternal western diet feeding for five consecutive generations in mice induces a gradual enhancement in fat mass and related metabolic diseases over generations. Strikingly, chow-diet-fed progenies from these multigenerational western-diet-fed males develop a “healthy” overweight phenotype that is not reversed after 4 subsequent generations. Mechanistically, sperm RNA microinjection experiments into zygotes suggest that sperm RNAs are sufficient for establishment but not for long-term maintenance of epigenetic inheritance of metabolic pathologies. Progressive and permanent metabolic deregulation induced by successive paternal western-diet-fed generations may contribute to the worldwide epidemic of metabolic diseases.
Bright Soluble Private Limited: a case study on accounting process
Authors: Dubblish Puneet , Bhatia Shikha 
Published: May 2020

Learning outcomes
Learning outcomes of this paper are to analyse, record and classify financial transactions; prepare unadjusted trial balance; record the adjustment and closing entries and prepare post-closing trial balance; and prepare financial statements.

Case overview/synopsis
The case aims to induce users to draw up financial statements from the details provided. The complete accounting process is covered through solving the case. The case follows a start-up company from its first set of financial transactions to preparing the first set of financial statements. The case will help in application of accounting concepts, principles and the processes for recording transactions and preparation of financial statements.

Complexity academic level
The case is best suited for senior undergraduate- and graduate-level students of management/business schools in the courses of introductory financial accounting, intermediate accounting and financial reporting.

Supplementary materials
Teaching notes are available for educators only.

Subject code
CSS 1: accounting and finance.
Does wind speed effects performance and cost of energy? A case study of wind farm
Authors: Hulio Zahid Hussain , Jiang Wei 
Published: May 2020

Purpose
The rapid rising of renewable energy sources particularly wind energy cannot be ignored. The numerical increase in wind energy farms throughout the world is the best example. The purpose of this paper is to assess the basic question of whether wind characteristics affect the performance and cost of energy. The importance of this question cannot be ruled out while comparing renewable energy to a conventional form of energy more specifically especially for the developing country where the cost of energy is very high.

Design/methodology/approach
The research design of this paper is consists of an assessment of local wind characteristics of the wind farm site using Weibull k and c parameters. The performance model is used to assess the performance of the wind turbine (WT) corresponding to local wind characteristics. The wind correlation with WT in terms of changing wind speed has been assessed to quantify the effects of wind speed on the WT behavior and failure of WT components. Similarly, the power curve of WT is assessed and compared with the International Electrotechnical Commission standards 61400-12-2. The WT power coefficient and tip speed ratio corresponding to wind speed is also investigated. The energy volume and cost of energy lost model is used to determine the cost and volume loss of energy/kWh of the wind farm.

Findings
The findings of practical wind farms showed that the wind conditions of the site are showing a strong tendency that can be determined from the results of Weibull k and c parameters. The k and c parameters are observed to be 3.44 and 9.16 m/s, respectively, for a period of a year. The standard deviation is observed to be 2.56 for a period of a year. WT shows the efficient behavior can be obtained from the power coefficient and tip speed of WT at different wind speeds. Also, wind farm observation showed that to be some increasing wind speed cause of based WT component failures. The results of energy volume and cost/kWh assessment showed that the major portion of energy volume and cost of energy is lost owing to network, voltage dip and frequency surge, electrical and mechanical components failures.

Originality/value
Generally, it can be concluded that the WTs are now able to cope with variable wind speeds. However, the results of this paper are showing that WT performance and availability decreased due to increased wind speeds. It can also be a reason to decreased volume and increase the cost of energy/kWh.
Correlates of tax registration compliance among self-employed in Denkyira corridor of Ghana
Authors: Adu Kofi Osei , Amponsah Stephen 
Published: May 2020

PurposeThis paper aims to investigate the correlates of tax registration among self-employed in Upper Denkyira East municipal and Upper Denkyira West district in Ghana.Design/methodology/approachIn all, 783 self-employed were interviewed and discriminant analysis was used to examine the factors that influence a self-employed person’s decision to register with Ghana Revenue Authority.FindingsThe study found that, age of the self-employed, number of times tax officers visit and inspect self-employed, experience in business, number of years of schooling, distance to tax office and estimated annual profit are important discriminants of tax registration compliance.Originality/valueTax registration is the fundamental element of tax compliance since any effort to exact compliance from taxpayers begins with registration with the tax authority. However, previous studies in the Ghanaian informal sector such as Baba (2010), Baba and Asante (2012) and Razak and Adafula (2013) have focused mainly on aggregate tax compliance (registration, filing, reporting and payment combined) without given specific attention to this fundamental component of tax compliance risk. Therefore, this study seeks to address the gap in the literature.
The effects of organizational justice dimensions on facets of job engagement
Authors: Deepa S.M. 
Published: May 2020

PurposeThis study aims to examine the relationship between four dimensions of organizational justice (distributive, procedural, interpersonal and informational) and the three facets of job engagement (physical, cognitive and emotional).Design/methodology/approachA cross-sectional approach was used to survey a sample of 281 professionals in information technology who resided in the southern part of India and varied on experience, age and gender. Data was analyzed by employing the partial least squares structural equation modeling (PLS-SEM) approach.FindingsThe result emphasizes the individual role of each justice dimension on three job engagement facets, which suggests that all four dimensions of organizational justice have positive effects on physical, cognitive and emotional job engagement.Originality/valueThe current study contributes to the literature by examining all three job engagement dimensions and provides evidence that distributive, procedural, interpersonal and informational justices are significant predictors of physical, cognitive and emotional job engagement.
Does an open-plan office actually work? A workplace gap analysis: importance and perceived support of key activities
Authors: Chacon Vega Ricardo Jose , Gale Stephen P. , Kim Yujin , Hong Sungil , Yang Eunhwa 
Published: May 2020

Purpose
This study aims to investigate the performance of open-plan office layouts and to identify occupants’ concerns in existing open-plan office layouts.

Design/methodology/approach
Workplace activity questionnaire (WAQ) was administered in the form of an online survey in March 2019, as part of a design briefing process for the expansion of the office facilities located in Bangalore, India, for a Fortune 100 software technology company. A total of 4,810 questionnaires were distributed and 3,877 responses were received (80.6% response rate). After that, 849 incomplete responses were eliminated from the analysis, resulting in a final sample size of 3,028. The questionnaire included 11 key activities conducted by the office workers and established the gap between the workers’ perceived importance and support from their existing facilities using a five-point Likert scale.

Findings
The findings of this study provide strong evidence that different physical environments influence the satisfaction of occupants. An improvement of the facilities, especially by enabling areas for quiet working, should be prioritized in relation to the other activities surveyed. Also, office workers perceived significantly different support levels for quiet working depending on their department, while there was no significant difference between the workers of different buildings.

Research limitations/implications
Individual demographic information was not collected because of the possibility of personal identification. There was also a lack of objective environmental measures, such as temperature and noise level. Thus, the quality of indoor environments was unknown. In this study, some respondents mentioned dissatisfaction with indoor environmental quality, including noise, temperature and air quality in their comments.

Originality/value
In the programming stage of a workplace design process, the WAQ survey tool has value because it renders important insight into the perception of a live workplace, which can then be used to determine priorities for a design effort. It clearly identifies the areas to focus on, ask questions about and develop improvements. Validating its reliability will enhance its credibility and confidence in its use. In addition, the large sample size provides statistical advantages in the data analysis, providing a higher likelihood to find a true positive of the findings of the study. Also, having a relatively high response rate provides an advantage of mitigating the risk of having non-response bias in the analysis.
Cluster development policies and firms’ performance: evidence from an emerging economy in Latin America
Authors: Aboal Diego , Perera Marcelo , Rovira Flavia 
Published: May 2020

Purpose
Impact evaluations of cluster programs at firm level are still scarce in the literature. The available evidence on the effectiveness of such programs based on rigorous quantitative impact evaluations is mixed. The purpose of this paper is to contribute to the body of literature the evaluates quantitatively the impact of cluster programs in emerging economies on firms’ performance. In particular, the authors evaluate the impact of a cluster program in Uruguay on firms’ sales and exports.

Design/methodology/approach
The authors use state-of-the-art impact evaluation methods to evaluate the impact of the program. In particular, difference in differences and matching methods

Findings
There is very strong evidence that the program had a positive impact on exports and the propensity to export of firms. However, the evidence of a positive impact on sales is weak. The evidence suggests that the maximum effect of the program can be found in the fourth or fifth year after the intervention.

Originality/value
The contribution of this paper to the literature is fourfold. First, this paper adds to the scarce body of literature evaluating the effects of cluster development programs with state-of-the-art impact evaluation methods. Second, it adds evidence for Latin America, a region that has implement a number of cluster policies (Maffioli et al., 2016) and where, as far as the authors know, there is only one additional paper evaluating rigorously the impacts of them (Figal-Garone et al., 2015). In addition, the authors provide evidence about the timing of the effects after the implementation of a cluster policy, an important issue that is mostly overlooked in the existent literature. Finally, the paper focus its attention on the impacts on exports and the propensity to export of firms, key elements for small open economies in Latin America that are heavily reliant on foreign currency inflows.
Revisiting cognitive assessment in the Indian prison setting
Authors: Aggarwal Pankhuri , Joseph Angela A. 
Published: May 2020

Purpose
Individuals with cognitive impairment are more likely to come into contact with the criminal justice system (Kimbell, 2016). Yet, only a handful of studies describe the nature of cognitive impairment experienced by inmates and the different types of challenges faced by researchers and clinicians while conducting cognitive assessments in correctional settings specifically in low-and middle-income countries.

Design/methodology/approach
In the present paper, the authors describe different types of ethical and logistical challenges they faced while conducting cognitive assessments with inmates in India and suggest ways in which future researchers and clinicians could overcome them.

Findings
Authors raise a discussion on the purpose, advantages, and limitations of psychological testing, highlighting alternative ways of cognitive assessment that may be more effective, resource-efficient, and sustainable.

Originality/value
Implications for education and training in psychological assessment, forensic and clinical practice and policymaking are discussed.
A literature review about the prevalence and identification of people with an intellectual disability within court Liaison and Diversion services
Authors: Marshall-Tate Karina , Chaplin Eddie , McCarthy Jane , Grealish Annmarie 
Published: May 2020

Purpose
Expert consensus is that people with an intellectual disability are over represented across the criminal justice setting (CJS). Primary research studies have been conducted in police stations and prisons, but little is known about the prevalence of this population in the court setting. The purpose of this paper is to conduct a literature review to find out more about the prevalence of defendants with an intellectual disability in court.

Design/methodology/approach
A literature review was conducted using standard systematic review methodology (Julian et al., 2011) and the PRISMA reporting guidelines (Moher et al., 2009).

Findings
Two papers met the inclusion criteria and were critically appraised. The papers reported prevalence findings ranging from 10%–20%.

Research limitations/implications
Differences in study design, sampling, recruitment and diagnostic criteria affect the ability to make comparisons or synthesise findings.

Practical implications
It is important that future primary and secondary research studies standardise operational terms to enable true comparison between studies, systematic reviews and evidence syntheses.

Social implications
Defendants with an intellectual disability need to be identified to enable criminal justice professionals to make reasonable adjustments to proceedings and consider diversion and alternative disposal options. This will likely improve outcomes for this population and reduce recidivism.

Originality/value
This literature review contributes to the growing evidence base about meeting the criminal justice needs of people with a learning disability and recognition of the increased prevalence across the CJS and specifically within the court setting.