bluuebunny/crossref_metadata_2025_split huggingface dataset:
A method to analyze the sensitivity ranking of various abiotic factors to acoustic densities of fishery resources in the surface mixed layer and bottom cold water layer of the coastal area of low latitude: a case study in the northern South China Sea
Authors: Sun Mingshuai , Cai Yancong , Zhang Kui , Zhao Xianyong , Chen Zuozhi 
Published: July 2020

AbstractThis is an exploratory analysis combining artificial intelligence algorithms, fishery acoustics technology, and a variety of abiotic factors in low-latitude coastal waters. This approach can be used to analyze the sensitivity level between the acoustic density of fishery resources and various abiotic factors in the surface mixed layer (the water layer above the constant thermocline) and the bottom cold water layer (the water layer below the constant thermocline). The fishery acoustic technology is used to obtain the acoustic density of fishery resources in each water layer, which is characterized by Nautical Area Scattering Coefficient values (NASC), and the artificial intelligence algorithm is used to rank the sensitivity of various abiotic factors and NASC values of two water layers, and the grades are classified according to the cumulative contribution percentage. We found that stratified or multidimensional analysis of the sensitivity of abiotic factors is necessary. One factor could have different levels of sensitivity in different water layers, such as temperature, nitrite, water depth, and salinity. Besides, eXtreme Gradient Boosting and random forests models performed better than the linear regression model, with 0.2 to 0.4 greater R2 value. The performance of the models had smaller fluctuations with a larger sample size.
Depression, neuroticism and 2D:4D ratio: evidence from a large, representative sample
Authors: Lautenbacher Leopold Maria , Neyse Levent 
Published: July 2020

AbstractA body of literature reports higher rates of depression and neuroticism in female samples compared to male samples. Numerous studies have investigated the role of prenatal sex hormone exposure in this sex difference, using the ratio between the second and fourth digit of the hand (“2D:4D”) as a putative marker. However, the sample sizes of those studies were mostly small and results remained inconclusive. The aim of the present study is to test the suggested associations between depression, neuroticism and the 2D:4D ratio in a large, representative sample of over 3,000 German individuals. It was hypothesized that a higher 2D:4D (supposedly representing a more “feminine” prenatal hormone exposure) would positively predict (1) one’s history of depression as well as (2) neuroticism rates and (3) acute depressive symptom scores. Controlling for biological sex, we only found suggestive evidence for linear associations with neuroticism in the case of left hand 2D:4D ratios and the mean 2D:4D of both hands. However, additional analyses indicated that these results may have been spurious due to confounding. Our findings suggest that the 2D:4D ratio is not a relevant predictor of depression, while there was mixed evidence in the case of neuroticism.
Inverse optimal control with time-varying objectives: application to human jumping movement analysis
Authors: Westermann Kevin , Lin Jonathan Feng-Shun , Kulić Dana 
Published: July 2020

AbstractAnalysis of complex human movements can provide valuable insights for movement rehabilitation, sports training, humanoid robot design and control, and human–robot interaction. To accomplish complex movement, the central nervous system must coordinate the musculo-skeletal system to achieve task and internal (e.g., effort minimisation) objectives. This paper proposes an inverse optimal control approach for analysing complex human movement that does not assume that the control objective(s) remains constant throughout the movement. The movement trajectory is assumed to be optimal with respect to a cost function composed of the sum of weighted basis cost functions, which may be time varying. The weights of the cost function are recovered using a sliding window. To illustrate the proposed approach, a dataset consisting of standing broad jump to targets at three different distances is collected. The method can be used to extract control objectives that influence task success, identify different motion strategies/styles, as well as to observe how control strategy changes during the motor learning process. Kinematic analysis confirms that the identified control objectives, including centre-of-mass takeoff vector and foot placement upon landing are important to ensure that a given participant lands on the target. The dataset, including nearly 800 jump trajectories from 22 participants is also provided.
Behavioural responses of white sharks to specific baits during cage diving ecotourism
Authors: Becerril-García Edgar E. , Hoyos-Padilla Edgar M. , Micarelli Primo , Galván-Magaña Felipe , Sperone Emilio 
Published: July 2020

AbstractThis study describes the effect of different baits on the attraction, surface behaviour and conditioning of white sharks Carcharodon carcharias during local ecotourism activities. The sightings, behaviours, and pictures used for photographic identification were obtained during August to November 2012–2014 onboard tourist boats in Guadalupe Island, Mexico. Four types of baits were used: (1) frozen bait; (2) frozen bait and natural chum; (3) fresh fish bait; and (4) mackerel bags. Data were analysed according to sex, maturity and the total of sharks using 6,145 sightings of 121 white sharks. The type of bait showed no significant difference on the effectiveness to attracting sharks. Ethological analysis showed that the type of bait had a significant effect on the shark’s surface behaviour during its interactions with boats. Natural chum and fresh baits showed short term behavioural patterns constituted by increased number of violent interactions with the bait, while the frozen bait did not generate a defined behavioural pattern. Conditioning of white sharks was determined by the number of interactions and the consumption frequency of the bait. Fifty nine percent of sharks (n = 41) showed no conditioning, 36% (n = 25) showed a low risk and only 5% (n = 3) were found to have a high risk of conditioning. The results suggest that current ecotourism has no effect on the conditioning of the white sharks, and that all baits have a similar effectiveness for attracting the sharks. However, a different behavioural pattern was observed when fresh bait and chum were used, which could increase the potential of accidents during ecotourism.
The osteogenic commitment of CD271+CD56+ bone marrow stromal cells (BMSCs) in osteoarthritic femoral head bone
Authors: Ilas Dragos C. , Baboolal Thomas G. , Churchman Sarah M. , Jones William G. , Giannoudis Peter V. , Bühring Hans-Jörg , McGonagle Dennis , Jones Elena 
Published: July 2020

AbstractOsteoarthritis (OA), the most common joint disorder, is characterised by progressive structural changes in both the cartilage and the underlying subchondral bone. In late disease stages, subchondral bone sclerosis has been linked to heightened osteogenic commitment of bone marrow stromal cells (BMSCs). This study utilised cell sorting and immunohistochemistry to identify a phenotypically-distinct, osteogenically-committed BMSC subset in human OA trabecular bone. Femoral head trabecular bone tissue digests were sorted into CD45-CD271+CD56+CD146-, CD45-CD271+CD56-CD146+ and CD45-CD271+CD56-CD146-(termed double-negative, DN) subsets, and CD45+CD271-hematopoietic-lineage cells served as control. Compared to the CD146+ subset, the CD56+ subset possessed a lower-level expression of adipocyte-associated genes and significantly over 100-fold higher-level expression of many osteoblast-related genes including osteopontin and osteocalcin, whilst the DN subset presented a transcriptionally ‘intermediate’ BMSC population. All subsets were tri-potential following culture-expansion and were present in control non-OA trabecular bone. However, while in non-OA bone CD56+ cells only localised on the bone surface, in OA bone they were additionally present in the areas of new bone formation rich in osteoblasts and newly-embedded osteocytes. In summary, this study reveals a distinct osteogenically-committed CD271+CD56+ BMSC subset and implicates it in subchondral bone sclerosis in hip OA. CD271+CD56+ subset may represent a future therapeutic target for OA and other bone-associated pathologies.
Digital and analogue modulation and demodulation scheme using vortex-based spin torque nano-oscillators
Authors: Jenkins Alex S. , Alvarez Lara San Emeterio , Freitas Paulo P. , Ferreira Ricardo 
Published: July 2020

AbstractIn conventional communications systems, information is transmitted by modulating the frequency, amplitude or phase of the carrier signal, which often occurs in a binary fashion over a very narrow bandwidth. Recently, ultra-wideband signal transmission has gained interest for local communications in technologies such as autonomous local sensor networks and on-chip communications, which presents a challenge for conventional electronics. Spin-torque nano-oscillators (STNOs) have been studied as a potentially low power highly tunable frequency source, and in this report we expand on this to show how a specific dynamic phase present in vortex-based STNOs makes them also well suited as Wideband Analogue Dynamic Sensors (WADS). This multi-functionality of the STNOs is the basis of a new modulation and demodulation scheme, where nominally identical devices can be used to transmit information in both a digital or analogue manner, with the potential to allow the highly efficient transmittance of data.
Morphological description of the first protozoeal stage of the deep-sea shrimps Aristeus antennatus and Gennadas elegans, with a key
Authors: Carreton Marta , Dos Santos Antonina , De Sousa Lígia Faria , Rotllant Guiomar , Company Joan B. 
Published: July 2020

AbstractAccurate information on commercial marine species larvae is key to fisheries science, as their correct identification is the first step towards studying the species’ connectivity patterns. In this study, we provide a complete morphological description of the first protozoeal stage of the valued deep-sea blue and red shrimpAristeus antennatusand of the small mesopelagic shrimpGennadas elegans. These two larval morphologies previously posed a risk of misidentification, thus hindering the study ofA. antennatuslarval ecology and dynamics in the context of fisheries science. Using specimens caught in the plankton at various locations in the Northwestern Mediterranean Sea and identification confirmed by molecular methods, the larvae ofA. antennatusandG. elegansare distinguished from each other by the ornamentation of the antennula. A possible confusion in previous descriptions of Aristeidae larvae is addressed and a new key for the identification of Dendrobranchiata larvae provided.
MTFMT deficiency correlates with reduced mitochondrial integrity and enhanced host susceptibility to intracellular infection
Authors: Seo Jung-Hwa , Hwang Cheol-Sang , Yoo Joo-Yeon 
Published: July 2020

AbstractMitochondria behave as functional and structural hubs for innate defense against intracellular infection. While the mitochondrial membrane serves as a platform for the assembly of signaling complexes activated by intracellular infection, various danger molecules derived from impaired mitochondria activate innate signaling pathways. Using methionyl-tRNA formyl transferase (MTFMT)-deficient cells, which exhibit impaired mitochondrial activity, we examined the role of mitochondrial integrity in regulating innate defense against infection. Since MTFMT functions at the early steps of mitochondrial translation, its loss was expected to cause defects in mitochondrial activity. Under transient MTFMT gene silencing conditions, we observed shortened mitochondria along with reduced activity. MTFMT-silenced cells were more susceptible to intracellular infection, as examined by infection with RNA viruses and the intracellular bacterium Shigella flexneri. In support of this observation, MTFMT-silenced cells possessed lowered basal NF-κB activity, which remained low after S. flexneri infection. In addition, the mitochondrial accumulation of evolutionarily conserved signaling intermediate in Toll pathway (ECSIT), an adaptor protein for NF-κB activation, was significantly decreased in MTFMT-silenced cells, explaining the reduced NF-κB activity observed in these cells. Since impaired mitochondria likely release mitochondrial molecules, we evaluated the contribution of mitochondrial N-formyl peptides to the regulation of bacterial infection. Transient transfection of mitochondrial-derived N-formyl peptides favored S. flexneri infection, which was accompanied by enhanced bacterial survival, but did not affect host cell viability. However, transient transfection of mitochondrial-derived N-formyl peptides did not affect basal NF-κB activity. Altogether, these data suggest that the integrity of mitochondria is essential to their proper function in protecting against infection, as intact mitochondria not only block the release of danger molecules but also serve as signaling hubs for the downstream NF-κB pathway.
Tumor microenvironment characterization in head and neck cancer identifies prognostic and immunotherapeutically relevant gene signatures
Authors: Huo Mengqi , Zhang Ying , Chen Zhong , Zhang Suxin , Bao Yang , Li Tianke 
Published: July 2020

AbstractThe tumor microenvironment (TME) is of great clinical significance for predicting the therapeutic effect of tumors. Nonetheless, there was no systematic analysis of cellular interactions in the TME of head and neck cancer (HNSC). This study used gene expression data from 816 patients with HNSC to analyze the scores of 22 immune cells. On this basis, we have established a novel TMEscore-based prognostic risk model. The relationship between TMEscore and clinical and genomic characteristics was analyzed. The sample was divided into risk-H and risk-L groups based on the prognosis risk model of TMEscore, with significant differences in overall survival between the two groups (log rank p < 0.001). In terms of clinical features, the TMEscore is closely related to the T staging, Grade, and HPV. As for genomic characteristics, the genomic features of the Risk-H samples are a low expression of immune-related genes and high-frequency mutations of TP53 and CEP152. This model was validated in an external test set, in which the prognosis for Risk-H group and Risk-L group was also significantly different (log rank p = 0.017). A quantitative method of TME infiltration pattern is established, which may be a potential predictor of HNSC prognosis.
Humanized GPRC6AKGKY is a gain-of-function polymorphism in mice
Authors: Pi Min , Xu Fuyi , Ye Ruisong , Nishimoto Satoru K. , Kesterson Robert A. , Williams Robert W. , Lu Lu , Quarles L. Darryl 
Published: July 2020

AbstractGPRC6A is proposed to regulate energy metabolism in mice, but in humans a KGKY polymorphism in the third intracellular loop (ICL3) is proposed to result in intracellular retention and loss-of-function. To test physiological importance of this human polymorphism in vivo, we performed targeted genomic humanization of mice by using CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats-CRISPR associated protein 9) system to replace the RKLP sequence in the ICL3 of the GPRC6A mouse gene with the uniquely human KGKY sequence to create Gprc6a-KGKY-knockin mice. Knock-in of a human KGKY sequence resulted in a reduction in basal blood glucose levels and increased circulating serum insulin and FGF-21 concentrations. Gprc6a-KGKY-knockin mice demonstrated improved glucose tolerance, despite impaired insulin sensitivity and enhanced pyruvate-mediated gluconeogenesis. Liver transcriptome analysis of Gprc6a-KGKY-knockin mice identified alterations in glucose, glycogen and fat metabolism pathways. Thus, the uniquely human GPRC6A-KGKY variant appears to be a gain-of-function polymorphism that positively regulates energy metabolism in mice.
Sodium ions allosterically modulate the M2 muscarinic receptor
Authors: Friedman Sheli , Tauber Merav , Ben-Chaim Yair 
Published: July 2020

AbstractG protein coupled receptors (GPCRs) play a key role in the vast majority of cellular signal transduction processes. Previous experimental evidence has shown that sodium ion (Na+) allosterically modulate several class A GPCRs and theoretical studies suggested that the same also holds true for muscarinic receptors. Here we examined, using Xenopus oocytes as an expression system, the effect of Na+ on a prototypical GPCR, the M2 muscarinic receptor (M2R). We found that removal of extracellular Na+ resulted in a decrease in the potency of ACh toward the M2R and that a conserved aspartate in transmembrane domain 2 is crucial for this effect. We further show that this allosteric effect of Na+ does not underlie the voltage-dependence of this receptor.
Combinational therapy with antibiotics and antibiotic-loaded adipose-derived stem cells reduce abscess formation in implant-related infection in rats
Authors: Yoshitani Junya , Kabata Tamon , Arakawa Hiroshi , Kato Yukio , Nojima Takayuki , Hayashi Katsuhiro , Tokoro Masaharu , Sugimoto Naotoshi , Kajino Yoshitomo , Inoue Daisuke , Ueoka Ken , Yamamuro Yuki , Tsuchiya Hiroyuki 
Published: July 2020

AbstractImplant-related infection is difficult to treat without extended antibiotic courses. However, the long-term use of antibiotics has led to the development of multidrug- and methicillin-resistant Staphylococcusaureus. Thus, alternatives to conventional antibiotic therapy are needed. Recently, mesenchymal stem cells have been shown to have antimicrobial properties. This study aimed to evaluate the antimicrobial activity and therapeutic effect of local treatment with antibiotic-loaded adipose-derived stem cells (ADSCs) plus an antibiotic in a rat implant-associated infection model. Liquid chromatography/tandem mass spectrometry revealed that ADSCs cultured in the presence of ciprofloxacin for 24 h showed time-dependent antibiotic loading. Next, we studied the therapeutic effects of ADSCs and ciprofloxacin alone or in combination in an implant-related infection rat model. The therapeutic effects of ADSCs plus antibiotics, antibiotics, and ADSCs were compared with no treatment as a control. Rats treated with ADSCs plus ciprofloxacin had the lowest modified osteomyelitis scores, abscess formation, and bacterial burden on the implant among all groups (P < 0.05). Thus, local treatment with ADSCs plus an antibiotic has an antimicrobial effect in implant-related infection and decrease abscess formation. Thus, our findings indicate that local administration of ADSCs with antibiotics represents a novel treatment strategy for implant-associated osteomyelitis.
A time-dependent diffusion MRI signature of axon caliber variations and beading
Authors: Lee Hong-Hsi , Papaioannou Antonios , Kim Sung-Lyoung , Novikov Dmitry S. , Fieremans Els 
Published: July 2020

AbstractMRI provides a unique non-invasive window into the brain, yet is limited to millimeter resolution, orders of magnitude coarser than cell dimensions. Here, we show that diffusion MRI is sensitive to the micrometer-scale variations in axon caliber or pathological beading, by identifying a signature power-law diffusion time-dependence of the along-fiber diffusion coefficient. We observe this signature in human brain white matter and identify its origins by Monte Carlo simulations in realistic substrates from 3-dimensional electron microscopy of mouse corpus callosum. Simulations reveal that the time-dependence originates from axon caliber variation, rather than from mitochondria or axonal undulations. We report a decreased amplitude of time-dependence in multiple sclerosis lesions, illustrating the potential sensitivity of our method to axonal beading in a plethora of neurodegenerative disorders. This specificity to microstructure offers an exciting possibility of bridging across scales to image cellular-level pathology with a clinically feasible MRI technique.
Circular RNA circFGD4 suppresses gastric cancer progression via modulating miR-532-3p/APC/β-catenin signalling pathway
Authors: Dai Xinglong , Liu Jianjun , Guo Xiong , Cheng Anqi , Deng Xiaoya , Guo Liqun , Wang Ziwei 
Published: July 2020

Abstract
Background: Mounting evidence has displayed critical roles of circular RNAs (circRNAs) in multiple cancers. The underlying mechanisms by which circFGD4 contributed to gastric cancer (GC) are still unclear.
Methods: The levels and clinical values of circFGD4 in GC patients were detected and analysed by quantitative real-time PCR. The biological roles of circFGD4 in GC were assessed in vitro and in vivo experiments. Dual-luciferase reporter, fluorescence in situ hybridization, RNA immunoprecipitation, biotin-coupled RNA pull-down, and TOP/Flash and FOP/Flash reporter gene assays were employed to evaluate the effects of circFGD4 on miR-532-3p-mediated adenomatous polyposis coli (APC)/β-catenin signalling in GC cells.
Results: circFGD4 expression was down-regulated the most in human GC tissues and cell lines. Low expression of circFGD4 was correlated with poor tumour differentiation, lymphatic metastasis, and poor prognosis of GC patients. circFGD4 suppressed GC cell viability, colony formation, migration, induced epithelial–mesenchymal transition (EMT), and tumorigenesis and metastasis in vivo. Next, we validated that circFGD4 acted as a sponge of miR-532-3p to relieve the tumour-promoting effects of miR-532-3p on its target APC. The mechanistic analysis demonstrated that the circFGD4 suppressed GC cell viability, migration, and EMT by modulating the miR-532-3p/APC axis to inactivate the β-catenin signalling.
Conclusion: circFGD4 suppressed GC progression through sponging miR-532-3p and enhancing APC expression to inactivate the β-catenin signalling. Thus circFGD4 provides a novel potential biomarker and valuable therapeutic strategy for GC.
A dynamical and radiation semi-analytical model of pulsar-star colliding winds along the orbit: Application to LS 5039
Authors: Molina E. , Bosch-Ramon V. 
Published: July 2020

Context.Gamma-ray binaries are systems that emit nonthermal radiation peaking at energies above 1 MeV. One proposed scenario to explain their emission consists of a pulsar orbiting a massive star, with particle acceleration taking place in shocks produced by the interaction of the stellar and pulsar winds.Aims.We develop a semi-analytical model of the nonthermal emission of the colliding-wind structure, which includes the dynamical effects of orbital motion. We apply the model to a general case and to LS 5039.Methods.The model consists of a one-dimensional emitter, the geometry of which is affected by Coriolis forces owing to orbital motion. Two particle accelerators are considered: one at the two-wind standoff location and the other one at the turnover produced by the Coriolis force. Synchrotron and inverse Compton emission is studied taking into account Doppler boosting and absorption processes associated to the massive star.Results.If both accelerators are provided with the same energy budget, most of the radiation comes from the region of the Coriolis turnover and beyond, up to a few orbital separations from the binary system. Significant orbital changes of the nonthermal emission are predicted in all energy bands. The model allows us to reproduce some of the LS 5039 emission features, but not all of them. In particular, the MeV radiation is probably too high to be explained by our model alone, the GeV flux is recovered but not its modulation, and the radio emission beyond the Coriolis turnover is too low. The predicted system inclination is consistent with the presence of a pulsar in the binary.Conclusions.The model is quite successful in reproducing the overall nonthermal behavior of LS 5039. Some improvements are suggested to better explain the phenomenology observed in this source, such as accounting for particle reacceleration beyond the Coriolis turnover, unshocked pulsar wind emission, and the three-dimensional extension of the emitter.