bluuebunny/crossref_metadata_2025_split huggingface dataset:
DNA methylation age calculators reveal association with diabetic neuropathy in type 1 diabetes
Authors:   DCCT/EDIC Research Group, Roshandel Delnaz , Chen Zhuo , Canty Angelo J. , Bull Shelley B. , Natarajan Rama , Paterson Andrew D. 
Published: April 2020

Abstract
Background
Many CpGs become hyper or hypo-methylated with age. Multiple methods have been developed by Horvath et al. to estimate DNA methylation (DNAm) age including Pan-tissue, Skin & Blood, PhenoAge, and GrimAge. Pan-tissue and Skin & Blood try to estimate chronological age in the normal population whereas PhenoAge and GrimAge use surrogate markers associated with mortality to estimate biological age and its departure from chronological age. Here, we applied Horvath’s four methods to calculate and compare DNAm age in 499 subjects with type 1 diabetes (T1D) from the Diabetes Control and Complications Trial/Epidemiology of Diabetes Interventions and Complications (DCCT/EDIC) study using DNAm data measured by Illumina EPIC array in the whole blood. Association of the four DNAm ages with development of diabetic complications including cardiovascular diseases (CVD), nephropathy, retinopathy, and neuropathy, and their risk factors were investigated.

Results
Pan-tissue and GrimAge were higher whereas Skin & Blood and PhenoAge were lower than chronological age (p < 0.0001). DNAm age was not associated with the risk of CVD or retinopathy over 18–20 years after DNAm measurement. However, higher PhenoAge (β = 0.023, p = 0.007) and GrimAge (β = 0.029, p = 0.002) were associated with higher albumin excretion rate (AER), an indicator of diabetic renal disease, measured over time. GrimAge was also associated with development of both diabetic peripheral neuropathy (OR = 1.07, p = 9.24E−3) and cardiovascular autonomic neuropathy (OR = 1.06, p = 0.011). Both HbA1c (β = 0.38, p = 0.026) and T1D duration (β = 0.01, p = 0.043) were associated with higher PhenoAge. Employment (β = − 1.99, p = 0.045) and leisure time (β = − 0.81, p = 0.022) physical activity were associated with lower Pan-tissue and Skin & Blood, respectively. BMI (β = 0.09, p = 0.048) and current smoking (β = 7.13, p = 9.03E−50) were positively associated with Skin & Blood and GrimAge, respectively. Blood pressure, lipid levels, pulse rate, and alcohol consumption were not associated with DNAm age regardless of the method used.

Conclusions
Various methods of measuring DNAm age are sub-optimal in detecting people at higher risk of developing diabetic complications although some work better than the others.
MSX2 suppression through inhibition of TGFβ signaling enhances hematopoietic differentiation of human embryonic stem cells
Authors: Wang Hongtao , Wang Mengge , Wang Yu , Wen Yuqi , Chen Xiaoyuan , Wu Dan , Su Pei , Zhou Wen , Shi Lihong , Zhou Jiaxi 
Published: April 2020

Abstract
Background
Strategies of generating functional blood cells from human pluripotent stem cells (hPSCs) remain largely unsuccessful due to the lack of a comprehensive understanding of hematopoietic development. Endothelial-to-hematopoietic transition (EHT) serves as the pivotal mechanism for the onset of hematopoiesis and is negatively regulated by TGF-β signaling. However, little is known about the underlying details of TGF-β signaling during EHT.

Methods
In this study, by applying genome-wide gene profiling, we identified muscle segment homeobox2 (MSX2) as a potential mediator of TGF-β signaling during EHT. We generated MSX2-deleted human embryonic stem cell (hESC) lines using the CRISPR/Cas9 technology and induced them to undergo hematopoietic differentiation. The role of MSX2 in hematopoiesis and functional regulation of TGFβ signaling in EHT was studied.

Results
We identified MSX2 as a novel regulator of human hematopoiesis. MSX2 deletion promotes the production of hematopoietic cells from hESCs. Functional and bioinformatics studies further demonstrated that MSX2 deletion augments hematopoietic differentiation of hESCs by facilitating EHT. Mechanistically, MSX2 acts as a downstream target of TGFβ signaling to mediate its function during EHT.

Conclusions
Our results not only improve the understanding of EHT, but may also provide novel insight into the efficient production of functional blood cells from hPSCs for regenerative medicine.
HIF1α overexpression enhances diabetic wound closure in high glucose and low oxygen conditions by promoting adipose-derived stem cell paracrine function and survival
Authors: Xu Jin , Liu Xiaoyu , Zhao Feng , Zhang Ying , Wang Zhe 
Published: April 2020

Abstract
Background
Adipose-derived stem cell (ADSC) transplantation is a promising strategy to promote wound healing because of the paracrine function of stem cells. However, glucose-associated effects on stem cell paracrine function and survival contribute to impaired wound closure in patients with diabetes, limiting the efficacy of ADSC transplantation. Hypoxia-inducible factor (HIF)1α plays important roles in wound healing, and in this study, we investigated the effects of HIF1α overexpression on ADSCs in high glucose and low oxygen conditions.

Methods
Adipose samples were obtained from BALB/C mice, and ADSCs were cultured in vitro by digestion. Control and HIF1α-overexpressing ADSCs were induced by transduction. The mRNA and protein levels of angiogenic growth factors in control and HIF1α-overexpressing ADSCs under high glucose and low oxygen conditions were analyzed by quantitative reverse transcription-polymerase chain reaction and western blotting. The effects of ADSC HIF1α overexpression on the proliferation and migration of mouse aortic endothelial cells (MAECs) under high glucose were evaluated using an in vitro coculture model. Intracellular reactive oxygen species (ROS) and 8-hydroxydeoxyguanosine (8-OHdG) levels in ADSCs were observed using 2,7-dichlorodihydrofluorescein diacetate staining and enzyme-linked immunosorbent assays, respectively. Apoptosis and cell cycle analysis assays were performed by flow cytometry. An in vivo full-thickness skin defect mouse model was used to evaluate the effects of transplanted ADSCs on diabetic wound closure.

Results
In vitro, HIF1α overexpression in ADSCs significantly increased the expression of vascular endothelial growth factor A, fibroblast growth factor 2, and C-X-C motif chemokine ligand 12, which were inhibited by high glucose. HIF1α overexpression in ADSCs alleviated high glucose-induced defects in MAEC proliferation and migration and significantly suppressed ADSC ROS and 8-OHdG levels, thereby decreasing apoptosis and enhancing survival. In vivo, HIF1α overexpression in ADSCs prior to transplantation significantly enhanced angiogenic growth factor expression, promoting wound closure in diabetic mice.

Conclusions
HIF1α overexpression in ADSCs efficiently alleviates high glucose-induced paracrine dysfunction, decreases oxidative stress and subsequent DNA damage, improves viability, and enhances the therapeutic effects of ADSCs on diabetic wound healing.
Dedifferentiated soft tissue leiomyosarcoma with heterologous osteosarcoma component: case report and review of the literature
Authors: Gaeta Raffaele , Matera Davide , Muratori Francesco , Roselli Giuliana , Baldi Giacomo , Campanacci Domenico Andrea , Franchi Alessandro 
Published: April 2020

Abstract
Background
Soft tissue dedifferentiated leiomyosarcoma with heterologous osteosarcomatous component is an extremely rare entity described in only few cases in the literature.

Case presentation
We report the case of a 65-year-old male patient who, after initial inadequate surgery of a tumor of the left forearm, developed local recurrence that was treated with neoadjuvant chemotherapy, surgery and postoperative radiation therapy. Histologically the tumor showed an abrupt separation of two different patterns. One component consisted of interlacing fascicles of spindle cells with cigar-shaped nuclei strongly positive for smooth muscle actin, desmin and H-caldesmon. The other component consisted of a high-grade pleomorphic sarcoma with osteoid and chondroid matrix production, which positive for SATB2. Thus, a final diagnosis of dedifferentiated leiomyosarcoma was rendered. Fifteen months after treatment, the patient presented further local and distant relapse with pulmonary metastases and died 23 months after the first presentation.

Discussion and conclusions
Dedifferentiated leiomyosarcoma is a highly malignant neoplasm with a poor outcome. Extensive sampling of soft tissue leiomyosarcomas is recommended to detect possible dedifferentiated areas as they represent a crucial prognostic parameter.
DFT-based low-complexity optimal cell ID estimation in NB-IoT
Authors: Savaux Vincent 
Published: April 2020

AbstractThis paper deals with cell identifier (ID) estimation for narrowband-Internet of things (NB-IoT) system. It is suggested to transform the usual maximum likelihood (ML) estimator expression in order to highlight a discrete Fourier transform (DFT), which can be computed with fast algorithms. Therefore, the proposed method is a DFT-based low-complexity cell ID estimator that can be qualified as optimal in the ML sense. The principle is extended to the practical case where the channel is unknown and must be estimated. In this scenario, the concentrated likelihood function needs to be maximized, in which the ML channel estimate is a function of the unknown cell ID parameter. This operation only involves a few additional multiplications. Simulation results reveal that the performance of the proposed method actually matches the optimal one of the ML cell ID estimator. Furthermore, the technique is robust to residual frequency offset up to several hundreds of Hertz. We also show that the mean square error of channel estimation reaches its Cramér-Rao bound (CRB).
Seamless clustering multi-hop routing protocol based on improved artificial bee colony algorithm
Authors: Zhang Tianyi , Chen Geng , Zeng Qingtian , Song Ge , Li Chao , Duan Hua 
Published: April 2020

AbstractAn important issue in the mobile sink wireless sensor networks (MSWSNs) is sensor energy optimization. In order to alleviate the problem of unbalanced network load and high energy consumption in MSWSNs, we proposed a new data collection protocol in this paper: seamless clustering multi-hop routing protocol based on improved artificial bee colony algorithm (IABCP). Because of limited by the communication sensing range and intelligence of ordinary nodes, routing paths can only be constructed by crude methods. And the movement of the sink node will generate a large amount of energy consumption for locating the sink node. In order to solve this problem, we assign the task of routing table generation to the sink node, which will generate the routing table through the improved artificial bee colony algorithm. In addition, we adopt a new method to select cluster head (CH) nodes; node uses the average energy of the surrounding nodes and its own residual energy to calculate the claimed cluster head time. Moreover, we added a sub cluster head CH-β node. When the CH node reaches the number of replacement rounds, the CH-β node becomes CH directly. The simulation results show that our routing protocol is more robust compared with three other protocols.
Discrete Hopfield neural network based indoor Wi-Fi localization using CSI
Authors: Dang Xiaochao , Tang Xuhao , Hao Zhanjun , Ren Jiaju 
Published: April 2020

AbstractThe fingerprint indoor localization method based on channel state information (CSI) has gained widespread attention. However, this method fails to provide a better localization effect and higher localization accuracy due to poor fingerprint accuracy, unsatisfactory classification and matching effect, and vulnerability to environmental impacts. In order to solve the problem, this paper proposes a CSI fingerprint indoor localization method based on the Discrete Hopfield Neural Network (DHNN). The method mainly consists of off-line and on-line phases. At the off-line phase, a low-pass filter is applied to conduct a preliminary processing on the fingerprint information of each reference point, and then, phase difference is adopted to correct the fingerprint data of all reference points. In this way, the quality of fingerprint data is improved, hence avoiding problems such as indoor environmental changes and multipath effect of signals, etc. in which impact the fingerprint data. Finally, the characteristic fingerprint database is established after acquiring relatively accurate fingerprint data. At the on-line phase, to maintain the consistency of data, the data of each reference point in the fingerprint database is set as an attractor. Meanwhile, the localization information of the test point is processed to make convergence judgment through DHNN. Eventually, the localization result is obtained. The experimental results show that the localization accuracy with a median error of 1.6 m can be achieved through the proposed method in the experimental environment. Compared with similar methods, it has a higher stability which can significantly reduce the cost of manpower and time.
Error reduction through post processing for wireless capsule endoscope video
Authors: Floor Pål Anders , Farup Ivar , Pedersen Marius , Hovde Øistein 
Published: April 2020

AbstractThe wireless capsule endoscope (WCE) is a pill-sized device taking images, which are transmitting to an on-body receiver, while traveling through the digestive system. Since image data is transmitted through the human body, which is a harsh medium for electromagnetic wave propagation, noise may at times heavily corrupt the reconstructed image frames. A common way to combat noise is to use error-correcting codes. In addition one may also utilize inter- and intra frame correlation to reduce the impact of noise at the receiver side, placing no extra demand on the WCE. However, it is then of great importance that the chosen post processing methods do not alter the content of the image as this can lead to miss-detection by gastroenterologists. In this paper we will investigate the possibility for additional noise suppression and error concealment at the receiver side in a high intensity error regime. Due to the high correlation generally inherent in WCE video, satisfactory results are obtained, as concluded from both subjective tests with gastroenterologists as well as the structural similarity (SSIM) metric. More surprisingly, the subjective tests indicate that the inpainted frames in many cases can be used for clinical assessment. These results indicate that one can apply error reduction through post processing together with error-correcting codes to obtain a more noise-robust system without any further demand on the WCE.
Mathematical evaluation of the role of cross immunity and nonlinear incidence rate on the transmission dynamics of two dengue serotypes
Authors: Janreung Sutawas , Chinviriyasit Wirawan , Chinviriyasit Settapat 
Published: April 2020

AbstractDengue fever is a common disease which can cause shock, internal bleeding, and death in patients if a second infection is involved. In this paper, a multi-serotype dengue model with nonlinear incidence rate is formulated to study the transmission of two dengue serotypes. The dynamical behaviors of the proposed model depend on the threshold value $R_{{0}}^{{n}}$R0n known as the reproductive number which depends on the associated reproductive numbers with serotype-1 and serotype-2. The value of $R_{{0}}^{{n}}$R0n is used to reflect whether the disease dies out or becomes endemic. It is found that the proposed model has a globally stable disease-free equilibrium if $R_{{0}}^{{n}}\leq 1$R0n≤1, which indicates that if public health measures that make (and keep) the threshold to a value less than unity are carried out, the strategy in disease control is effective in the sense that the number of infected human and mosquito populations in the community will be brought to zero irrespective of the initial sizes of sub-populations. When $R_{{0}}^{{n}}>1$R0n>1, the endemic equilibria called the co-existence primary and secondary infection equilibria are locally asymptotically stable. The effects of cross immunity and nonlinear incidence rate are explored using data from Thailand to determine the effective strategy in controlling and preventing dengue transmission and reinfection.
Energy-efficient sensory data gathering based on compressed sensing in IoT networks
Authors: Du Xinxin , Zhou Zhangbing , Zhang Yuqing , Rahman Taj 
Published: April 2020

AbstractThe Internet of Things (IoT) networks have become the infrastructure to enable the detection and reaction of anomalies in various domains, where an efficient sensory data gathering mechanism is fundamental since IoT nodes are typically constrained in their energy and computational capacities. Besides, anomalies may occur occasionally in most applications, while the majority of time durations may reflect a healthy situation. In this setting, the range, rather than an accurate value of sensory data, should be more interesting to domain applications, and the range is represented in terms of the category of sensory data. To decrease the energy consumption of IoT networks, this paper proposes an energy-efficient sensory data gathering mechanism, where the category of sensory data is processed by adopting the compressed sensing algorithm. The sensory data are forecasted through a data prediction model in the cloud, and sensory data of an IoT node is necessary to be routed to the cloud for the synchronization purpose, only when the category provided by this IoT node is different from the category of the forecasted one in the cloud. Experiments are conducted and evaluation results demonstrate that our approach performs better than state-of-the-art techniques, in terms of the network traffic and energy consumption.
Effect of Lamellar Inorganic Fillers on the Properties of Epoxy Emulsion Cement Mortar
Authors: Li Huabing , Zuo Jiandong , Dong Biqin , Xing Feng 
Published: April 2020

AbstractLamellar inorganic fillers have been used to improve the performance of polymer composites. In this paper, five kinds of lamellar inorganic fillers, including montmorillonite (MMT), mica, talc, glass flake (GF) and lamellar double hydroxide (LDH), were selected to modify epoxy emulsion cement mortar (EECM). The research evaluated the effects of the structure characteristics of lamellar fillers on the mechanical properties, water absorption and chloride ion permeability resistance of EECM, with comparison to granular ground calcium carbonate (GCC). Results indicated that lamellar fillers had no obvious superiority than GCC in the mechanical strength of EECM, even MMT caused the decline of the mechanical strength. However, lamellar fillers had improved the chloride ion permeability resistance of EECM compared with GCC, and they had the similar effect on reducing of water absorption except MMT. Although the low aspect ratio (AR) of the lamellar fillers benefited the increase of the strength and water resistance of EECM, the lamellar fillers with higher AR could improve the chloride ion permeability resistance of EECM more efficiently.
Ground golf-induced changes in the blood pressure of healthy elderly people
Authors: Ebine Naoyuki , Itoh Masahiro , Horiuchi Masahiro , Hojo Tatsuya , Yoshimura Miho , Fukuoka Yoshiyuki 
Published: April 2020

AbstractBackgroundGround golf is a popular sport among the elderly in Japan. Several types of exercise can reduce the body's mean arterial pressure (MAP), but little is known about how ground golf affects the MAP. We investigated the effects of ground golf on the MAP and the oxygen uptake ($$ \dot{\mathrm{V}}{\mathrm{O}}_2 $$V̇O2) in a healthy elderly population.Participants and methodsThirteen elderly Japanese people (3 males and 10 females, mean age of 66 years) participated. All participants played 8 holes of ground golf 6 times, as game (G)1 to G6. The MAP, heart rate (HR), and$$ \dot{\mathrm{V}}{\mathrm{O}}_2 $$V̇O2were measured at rest and every 5 min during each game.ResultsA linear trend analysis revealed that participants’ MAP values progressively decreased as each game proceeded with marginal differences (p= 0.054). There were no significant differences in HR between at rest and any of the games. The$$ \dot{\mathrm{V}}{\mathrm{O}}_2 $$V̇O2during the games (except for G6) were significantly higher than that at-rest (p< 0.05). The resting MAP values were negatively associated with the ground golf-induced changes in MAP (r= 0.786,p= 0.001). The participants with greater changes in$$ \dot{\mathrm{V}}{\mathrm{O}}_2 $$V̇O2during the games showed significantly greater reductions in MAP (r= 0.276,p= 0.043).ConclusionsPlaying ground golf reduced the participants’ MAP and increased their$$ \dot{\mathrm{V}}{\mathrm{O}}_2 $$V̇O2. Participants with higher resting MAP experienced greater reductions in MAP by playing ground golf, which suggests that ground golf can be a useful recreational sport for the elderly.
Annexin A2–STAT3–Oncostatin M receptor axis drives phenotypic and mesenchymal changes in glioblastoma
Authors: Matsumoto Yuji , Ichikawa Tomotsugu , Kurozumi Kazuhiko , Otani Yoshihiro , Fujimura Atsushi , Fujii Kentaro , Tomita Yusuke , Hattori Yasuhiko , Uneda Atsuhito , Tsuboi Nobushige , Kaneda Keisuke , Makino Keigo , Date Isao 
Published: April 2020

AbstractGlioblastoma (GBM) is characterized by extensive tumor cell invasion, angiogenesis, and proliferation. We previously established subclones of GBM cells with distinct invasive phenotypes and identified annexin A2 (ANXA2) as an activator of angiogenesis and perivascular invasion. Here, we further explored the role of ANXA2 in regulating phenotypic transition in GBM. We identified oncostatin M receptor (OSMR) as a key ANXA2 target gene in GBM utilizing microarray analysis and hierarchical clustering analysis of the Ivy Glioblastoma Atlas Project and The Cancer Genome Atlas datasets. Overexpression of ANXA2 in GBM cells increased the expression of OSMR and phosphorylated signal transducer and activator of transcription 3 (STAT3) and enhanced cell invasion, angiogenesis, proliferation, and mesenchymal transition. Silencing of OSMR reversed the ANXA2-induced phenotype, and STAT3 knockdown reduced OSMR protein expression. Exposure of GBM cells to hypoxic conditions activated the ANXA2–STAT3–OSMR signaling axis. Mice bearing ANXA2-overexpressing GBM exhibited shorter survival times compared with control tumor-bearing mice, whereas OSMR knockdown increased the survival time and diminished ANXA2-mediated tumor invasion, angiogenesis, and growth. Further, we uncovered a significant relationship between ANXA2 and OSMR expression in clinical GBM specimens, and demonstrated their correlation with tumor histopathology and patient prognosis. Our results indicate that the ANXA2–STAT3–OSMR axis regulates malignant phenotypic changes and mesenchymal transition in GBM, suggesting that this axis is a promising therapeutic target to treat GBM aggressiveness.