bluuebunny/crossref_metadata_2025_split huggingface dataset:
Androgen Receptor Regulates the Proliferation of Stretched Myoblasts and Its Mechanisms: IGF-1R-mediated p38 and ERK1/2 Pathways
Authors: Fu Shaoting , Lin Xiaojing , Yin Lijun , Wang Xiaohui 
Published: December 2020

Abstract
BackgroundOur previous study has indicated that in C2C12 myoblasts androgen receptor (AR) plays a crucial role in 15% stretch-induced proliferation through insulin-like growth factor (IGF-1)-modulated PI3K/Akt, p38 and ERK1/2 pathways. In this study we further explored AR's effects on 15% and 20% stretches-modulated myoblasts proliferation and involved pathways in L6 (no detectable AR) and C2C12 (abundant AR) myoblasts.MethodsThe proliferation and the above indicators were compared between stretched L6 and C2C12 myoblasts by CCK8, ELISA and Western blot, then were detected again in stretched L6 myoblasts after transfection with AR overexpression plasmid utilizing LipoPlusTM 2000 reagent or treatment with different concentrations (200, 500, 1000 ng/ml) IGF-1 recombinant polypeptide.ResultsWe found that ①Under un-stretched states, the proliferation rate of L6 cells was slower than that of C2C12 cells, and the proliferation of C2C12 cells was repressed by AR knockdown using siRNA interference, while L6 myoblasts proliferation was promoted by overexpression AR. ②The proliferation rate as well as the activities of p38 and ERK1/2 increased by 15% stretch were much lower in L6 than that in C2C12 cells; AR overexpression enhanced the proliferation of 15% stretched L6 cells accompanied with increased activities of p38 and ERK1/2; ③20% stretch decreased the proliferation and activity of p38 in L6 more than that in C2C12 myoblasts; overexpression of AR totally reversed the anti-proliferation of 20% stretch and significantly enhanced p38 activity in L6 myoblasts;④In L6 myoblasts, overexpression AR increased IGF-1R expression despite no detectable IGF-1 was secreted; and recombinant IGF-1 increased not only the proliferation, but also the protein level of IGF-1R and activities of p38 and ERK1/2 in a dose-dependent manner in 15% stretched L6 myoblasts. ConclusionsAR fulfilled 15% stretch-induced pro-proliferation of myoblasts by activating IGF-1R-p38 and ERK1/2 pathways, while 20% stretch-induced anti-proliferation was mediated by inhibiting AR-IGF-1R-p38 pathway. This study is beneficial to understand in depth the role and mechanisms of AR on appropriate exercise increases while excessive exercise decreases muscle mass.
The Heterogeneity of Intraductal Carcinoma of the Prostate Is Associated With Different Efficacy of Standard First-Line Therapy for Patients With Metastatic Castration-Resistant Prostate Cancer
Authors: Wang Zhipeng , Zhu Sha , Zhao Jinge , Nie Ling , Chen Xueqin , Zhang Mengni , Chen Ni , Sun Guangxi , Chen Junru , Ni Yuchao , Dai Jingdong , Liu Zhenhua , Tao Ronggui , Zhang Xingming , Zhu Xudong , Zhang Haoran , Liang Jiayu , Wang Zilin , He Ben , Shen Pengfei , Zeng Hao 
Published: December 2020

Abstract
BackgroundTo explore whether patients with distinct intraductal carcinoma of the prostate (IDC-P) subtypes respond differently to standard first-line therapy among patients with metastatic castration resistant prostate cancer (mCRPC).MethodsWe retrospectively analyzed data of 170 mCRPC patients receiving abiraterone (ABI) or docetaxel (DOC) as first-line therapy between 2014 and 2019. PSA response, PSA progression-free survival (PSA-PFS), radiographic progression-free survival (rPFS), and overall survival (OS) were analyzed and compared based on the presence of IDC-P and its sub-patterns.ResultsTotally, IDC-P was confirmed in 91/170 (53.5%) patients. Among them, 36/91 (39.6%) and 55/91 (60.4%) harbored IDC-P pattern 1 and pattern 2, respectively. The presence of IDC-P was confirmed to be associated with poor prognosis in the whole cohort. Patients with IDC-P pattern 1 shared similar clinical outcomes to those without IDC-P in both ABI and DOC treatment. However, compared to patients with IDC-P pattern 1 and without IDC-P, IDC-P pattern 2 had markedly poorer prognosis in either ABI (PSA-PFS: P<0.001; rPFS: P<0.001) or DOC (PSA-PFS: P<0.001; rPFS: P<0.001) treatment. For patients without IDC-P, DOC had comparable therapeutic efficacy with ABI. In contrast, the therapeutic efficacy of DOC in patients with either IDCP pattern 1 (PSA-PFS: P=0.021; rPFS: P=0.027) or pattern 2 (PSA-PFS: P=0.003; rPFS: P=0.007) was significantly inferior to ABI.ConclusionCompared to DOC, ABI exhibited better efficacy in patients with IDC-P of either pattern. However, IDC-P pattern 2 still responded unsatisfactorily to either ABI or DOC therapy. Novel therapeutic strategies appropriate for IDC-P pattern 2 need to be further investigated in the future.
Modification of the Interface Between Degradable Magnesium Alloy and Physiological Environment for Controlled Biodegradation Using Fibrous Polymer/Metal-Organic Framework Nanocomposite Coatings
Authors: Khalili Mohammad Amin , Tamjid Elnaz 
Published: December 2020

Abstract
Magnesium-based implants (MBIs) have recently attracted great attention in bone regeneration due to elastic modulus similar to bone. Nevertheless, the degradation rate and hydrogen release of MBIs in the body have to be tackled for practical applications. In the present study, we present a metal organic framework (MOF) nanoplates to reduce the degradation rate of AZ91 magnesium alloy. Zeolitic imidazolate frameworks (ZIF-8) with specific surface area of 1789 m2.g-1 were prepared by solvothermal methods, and after dispersion in a chitosan solution (10%w/w), the suspesnsion was electrospun on the surface of AZ91 alloy. Studying of the degradation rate in simulated body fluid (SBF) by electrochemical analysis including potentiodynamic polarization and electrochemical impedance spectroscopy reveals that the degradation rate of the surface modified implants is ~20 times less than the unmodified specimens. The reduced alkalization of the physiological environment and hydrogen release due to the implant degradation are shown. In vitro studies by fibroblasts and MG63 osteosarcoma cells exhibit improved cell adhesion and viability. The mechanisms behind the improved degradiation resistance and enhanced bioactivity are presented and discussed. Surface modification of MBIs by MOF-chitosan coatings is a promosing strategy to control the biodegradation of magnesium implants for bone regeneration.
Sleep During Infancy, Inhibitory Control and Working Memory in Toddlers: Findings from the FinnBrain Cohort Study
Authors: Muñoz Isabel Morales , Nolvi Saara , Mäkelä Tiina , Eskola Eeva , Korja Riikka , Fernandes Michelle , Karlsson Hasse , Paavonen E Juulia , Karlsson Linnea 
Published: December 2020

Abstract
Background: Sleep difficulties are associated with executive functioning (EF) impairment in school-aged children. However, much less is known about how sleep in infancy relates to EF in infants and/or toddlers. The aim of this study was to investigate whether parent-reported sleep patterns in infants at 6 and 12 months of age were associated with inhibitory control (IC) and working memory (WM) performances at 30 months. Methods: The children were divided into three sleep groups (i.e., “bad sleepers”, “intermediate sleepers” and “good sleepers”) based on percentile cut-off points in order to have a comprehensive understanding of the direction and nature of the associations between sleep and aspects of EF in early childhood. Sleep was assessed using the Brief Infant Sleep Questionnaire, IC was measured using a modified version of the Snack Delay task (N=425), and WM by using the Spin the Pots task (N=430). Results: Our results reported an inverted U-shaped association between proportion of daytime sleep at 12 months and IC at 30 months, indicating that average proportions of daytime sleep were longitudinally associated with better IC performance. Furthermore, a linear relation between time awake during night at 12 months and WM at 30 months was found, with more time awake at night associating with worse WM. Conclusions: Our findings support the hypothesis that sleep disruption in early childhood is associated with the development of later EF and suggest that different sleep difficulties at 12 months distinctively affect WM and IC in toddlers, possibly also in a non-linear manner.
New Ther1-derived SINE Squam3 in Scaled Reptiles
Authors: Vassetzky Nikita S , Kosushkin Sergei A , Korchagin Vitaly I , Ryskov Alexey P 
Published: December 2020

Abstract
Background
SINEs compose a significant part of animal genomes and are used to study the evolution of taxa. Despite significant advances in SINE studies in vertebrates and higher eukaryotes in general, their own evolution is poorly understood.
Results
We have found and described in detail a new SINE family Squam3 specific for scaled reptiles (Squamata). The subfamilies of this SINE demonstrate different distribution in the genomes of squamates, which together with the data on similar SINEs in the tuatara allowed us to propose a scenario of their evolution in the context of reptilian evolution.
Conclusions
Ancestral SINEs preserved in small numbers in most genomes can give rise to taxon-specific SINE families. Analysis of this aspect of SINEs can shed light on the history and mechanisms of SINE variation in reptilian genomes.
WITHDRAWN: Determinants of Market Participation and Intensity of Marketed Surplus Among Maize Producers in Fogera District of South Gondar Zone, Ethiopia: Heckman Two Stage Equation Approach.
Authors: Beadgie Walelgn Yalew , Reddy Ponguru 
Published: December 2020

Abstract
The agricultural productivity is low due to use of low level of improved agricultural technologies, risks associated with or no access to market facilities and low participation of the smallholder farmers. Hence the study focused on the specific objectives were to identify factors that affect market participation decision of households and to determine factors affecting the volume of market supply of maize. Quantitative and qualitative data were collected from primary and secondary sources. The cross-sectional survey was conducted using structured questionnaire, key informant interviews, and focus-group discussions. A stratified stage sampling technique was used to draw 150 sample units using systematic random sampling technique. Descriptive statistics and Heckman Two-Stage model was employed. The result indicated that 56% of market participant were male headed, while 30.67% were female headed. Whereas 15.58% of non-market participants were male headed households, while 5.84% of non-market participants were female headed households. Out of 17 potential variables, seven variables age, area of maize, oxen number, distance to market, access to market information, member of cooperatives and inverse mill’s ratio were significantly influence the decision & extent participation in maize marketing. Therefore, the following points are recommended to develop sustainable production and marketing of maize that is locally adaptable and acceptable to increase the competitiveness of smallholder farmers: improving access to credit to apply fertilizer, farmers should rely on intensive cultivation rather than extensive cultivation and strengthen extension service.
The Effect of miR-539 Regulating TRIAP1 on the Apoptosis, Proliferation, Migration and Invasion of Osteosarcoma Cells
Authors: Liu Huowen , Yang Min , Zhang Yufeng , Yang Zhiqiang , Chen Zhe , Xie Yuanlong , Peng Binglong , Cai Lin 
Published: December 2020

Abstract
Objective
The purpose of this study is to explore the effect of miRNA-539 on osteosarcoma and the underlying mechanism, so as to find a new method for early diagnosis and treatment of osteosarcoma.
Method
miRNA-539 mimics was transfected into osteosarcoma cells 143b and MG-63 and upregulated the expression of miR-539. QT-PCR was used to detect transfection efficacy. CCK-8 method was used to detect proliferation of 143b and MG-63 osteosarcoma cells and flow cytometry was used to detect the apoptosis of osteosarcoma cells 143b and MG-63. Wound-healing test and Transwell test were used to detect the migration and invasion ability of osteosarcoma cells. TRIAP1 was found to be the potential target gene of miRNA-539 by online bioinformatics software and the expression level of TRIAP1 in osteosarcoma cells overexpressing miRNA-539 was detected by qT-PCR. Western blot was used to detect the level of expression of TRIAP1 and its downstream genes (p53, p21, apfa1 and caspase9) in osteosarcoma cells 143b and MG63 transfected with miR-539 mimics or miR-539 mimics-NC. A model of osteosarcoma subcutaneously transplanted in nude mice was constructed to observe the effect of miRNA-539 on the growth rate of osteosarcoma in vivo.
Results
After transfection of miRNA-539 mimics in osteosarcoma cells 143b and MG63, the proliferation level, migration ability, and invasion ability of the osteosarcoma cells were significantly lower than that in the control group, and the apoptosis level was significantly higher than that in the control group (P < 0.01). The dual luciferase reporter confirmed that TRIAP1 was the target of miR-539, and the expression level of TRIAP1 in 143b and MG63 transfected with miRNA-539 mimics was proved to be significantly lower than that in the control group (P < 0.01).The western blot showed the expression of genes targeted by TRIAP1 was upregulated when the expression of TRIAP1 was downregulated. In vivo, the osteosarcoma growth rate in the miRNA-539 mimics group was significantly slower than that in the control group (P < 0.01).
Conclusion
MiRNA-539 may inhibit the cell proliferation, migration and invasion of osteosarcoma cells and promote the apoptosis of osteosarcoma cells by targeting on TRIAP1.
Identification of a Glycolysis-Related Gene Signature for Survival Prediction of Ovarian Cancer Patients
Authors: Zhang Dai , Yang Si , Li Yiche , Wang Meng , Yao Jia , Zheng Yi , Deng Yujiao , Li Na , Wei Bajin , Wu Ying , Zhai Zhen , Dai Zhijun , Kang Huafeng 
Published: December 2020

Abstract
Background: Ovarian cancer (OV) is deemed as the most lethal gynecological cancer in women. The aim of this study was construct an effective gene prognostic model for OV patients.Methods: The expression profiles of glycolysis-related genes (GRGs) and clinical data of patients with OV were extracted from The Cancer Genome Atlas (TCGA) database. Univariate, multivariate, and least absolute shrinkage and selection operator Cox regression analyses were conducted, and a prognostic signature based on GRGs was constructed. The predictive ability of the signature was analyzed in training and test sets.Results: Based on nine GRGs (ISG20, CITED2, PYGB, IRS2, ANGPTL4, TGFBI, LHX9, PC, and DDIT4), a gene risk signature was identified to predict the outcome of patients with OV. The signature showed a good prognostic ability for OV, particularly high-grade OV, in the TCGA dataset, with areas under the curve of 0.709, 0.762, and 0.808 for 3-, 5- and 10-year survival, respectively. Similar results were found in the test sets, and the signature was also an independent prognostic factor. Moreover, a nomogram combining the prediction model and clinical factors was constructed.Conclusion: Our study established a nine-GRG risk model and a nomogram to better perform on OV patients’ survival prediction. The risk model represents a promising and independent prognostic predictor for OV patients. Moreover, our study of GRGs could offer guidance for underlying mechanisms explorations in the future.
Laboratory and Field Performance of Five Cost-Effective Commercial Light Traps for Capturing Mosquitoes In China
Authors: Huang Run , Song Hongyun , Fang Qian , Qian Junping , Zhang Yaodan , Jiang Hong 
Published: December 2020

Abstract
BackgroundMosquito traps for household use are popular because they are small, cost-effective, user friendly, and environmentally friendly. At present, there are many variations and specifications of mosquito traps intended for household use on the market. Their labels claim they are powerful, but research and evaluation of their claims is lacking.MethodsThis article tested the key parameters, the laboratory capture rate, and the greenhouse field capture rate of 5 popular mosquito traps intended for household use,and compared them with the BG-trap, used by professionals to monitor mosquitoes in the field.ResultsThe study found that the wavelength of 395–400 nm had a better capture rate for Culex quinquefasciatus. In the laboratory experiment, the capture rate was between 34.7%-65.0%. The analysis showed that the total radiance, fan speed, and design of the air guide of the traps are important factors that affect the mosquito catch rate. Field tests in the greenhouse found that the 5 mosquito traps had low catch rates for Aedes albopictus. The average percentage of Cx. quinquefasciatus, Ae. albopictus, Anopheles Sinensis, and other flying insects captured every night was 51.76%, 25.29%, 14.12%, and 8.82%. There was no significant difference in the capture rate of Ae. albopictus by the 5 mosquito traps in the greenhouse, while the mosquito species captured during the same period by the human landing catch method were all Ae. albopictus, suggesting that the dominant species of mosquitoes in the greenhouse was Ae. albopictus. The comparison experiment of mosquito trap 5, with the highest capture rate in the laboratory simulation and greenhouse site, and the BG-trap in the morning, afternoon, and night showed that the capture rate of the BG-trap on Ae. albopictus and Cx. quinquefasciatus was higher than that of mosquito trap 5. Combined with the results of the human landing catch method during the same period, it showed that the BG-trap can more accurately reflect the composition of the mosquito community.ConclusionsAccording to this study, it is suggested that the current 395–400 nm wavelength mosquito traps are not suitable for mosquito control measures in the domestic indoor environment where Ae. albopictus is the dominant species. The mosquito traps intended for household use can be improved by increasing the fan speed and optimizing the air guide. With a higher catch rate, the BG-trap is more suitable for mosquito monitoring than the UV-trap.
Prevalence of Paradoxical Psoriasis after Exposure to Tumor Necrosis Factor Inhibitors (TNFi) in Children - Results from A Single Tertiary Center
Authors: Kodama Sarah , Gupta Deepti , Sullivan Erin , Rosenwasser Natalie , Zhao Yongdong 
Published: December 2020

Abstract
Objective: Paradoxical psoriasis has been increasingly reported in adults after exposure to tumor necrosis factor inhibitors (TNFi). Systematic studies in pediatric population are lacking. We aimed to investigate the relationship between TNFi therapy and the onset of new psoriasis in children. Methods: Patients with diagnosis of chronic nonbacterial osteomyelitis (CNO), juvenile idiopathic arthritis (JIA), or inflammatory bowel disease (IBD) treated with TNFi between 2005 and 2015 were identified. Baseline characteristics were compared among those developing psoriasis versus those who did not using t-tests or Wilcoxon Rank Sum tests. Results: A total of 1,092 patients were included with a median follow up of 21-36 months for CNO, JIA and IBD after the initiation of first TNFi. Psoriasis developed after exposure to TNFi in 4 of 28 CNO patients (14%), 3 of 620 JIA patients (0.5%) and 25 of 450 IBD patients (5.6%). There was no significant difference in distribution of age, gender, family history of psoriasis or concomitant medications between psoriasis subset and non-psoriasis subset. Among those who developed psoriasis, 38% had partial and 47% had complete responses to topical therapies or phototherapy. Eleven patients (35%) discontinued, none (0%) switched and twenty (65%) continued TNFi therapies. Conclusion: Cumulative incidence rates of TNFi-associated psoriasis varied in three underlying diseases. Increasing awareness of this unwanted side effect in pediatric community is important to ensure timely diagnosis and treatment. Fortunately , the majority of children affected had a favorable outcome.
Study Problems and Depressive Symptoms in Adolescents During the COVID-19 Outbreak: Poor Parent-child Relationship as a Vulnerability
Authors: Wang Jingyi , Wang Hao , Lin Haijiang , Richards Marcus , Yang Shuyue , Liang Hongbiao , Chen Xiaoxiao , Fu Chaowei 
Published: December 2020

Abstract
Background Little is known about the prevalence of and risk factors for adolescent mental health problems during the COVID-19 outbreak. We aimed to investigate the prevalence of depressive symptoms, their association with study-relevant problems, and the moderating effect of parent-child relationship among Chinese adolescents during the school closures.Results Using data from 6435 adolescents, we found that the prevalence of depressive symptoms was 17.7%. All the study problem measures were associated with more severe depressive symptoms. There was a moderating effect of the parental relationship on the associations between study problems and depressive symptoms. The association between number of study problems and depressive symptoms was stronger in adolescents with a poor parent-child relationship (regression coefficient 4.34 [95% CI 2.97, 5.72]) than those with a good or normal relationship (2.55 [2.35, 2.75]), p for interaction 0.002, on multivariable adjustment.Conclusions Study problems due to school closures were particularly problematic for adolescents who had poor parent-child relationships. Public health initiatives could help students to adjust study habits and improve parent-child relationships, thereby protecting against the development of depression.
Postoperative Air Leaks With Prompt Improvement of Low Suction on Digital Drainage Devices: a Retrospective Study
Authors: Mitsui Suguru , Tauchi Shunsuke , Uchida Takahiro , Ohnishi Hisashi , Shimokawa Toshio , Tobe Satoshi 
Published: December 2020

Abstract
Purpose: The aim was to investigate the most effective suction pressure for preventing or promptly improving postoperative air leaks.Methods: We retrospectively analyzed the postoperative data of 242 patients who were monitored with a digital drainage system after pulmonary resection between December 2017 and June 2020. We divided the patients into 3 of group by suction pressure, A (Low-pressure suction group: -5 cm H₂O), B (Intermediate-pressure group: -10 cm H₂O), C (High-pressure suction group: -20 cm H₂O). Duration of air leaks, duration of chest tube replacement, the amount of postoperative air leak, fluid volume drained before chest tube removal, and the maximum amount of air leaks during drainage were evaluated.Results: A total 217 patients were included. In the order of A, B, and C groups, duration of air leaks gradually decreased and significant trend was observed (p=0.019). Duration of chest tube replacement did not significantly differ among the three groups (p=0.126). The amount of postoperative air leak just after surgery did not significantly differ among the three groups (p=0.175), however, the amount of postoperative day 1 air leak gradually decreased with statistical significance in order of A, B, and C groups (p=0.033). The maximum amount of air leaks during drainage gradually decreased in order of A, B and C groups (p=0.036). Fluid volume drained before chest tube removal did not significantly differ among the three groups (p=0.986).Conclusion: Low-pressure suction after pulmonary resection would be useful for preventing or promptly improving postoperative air leaks.