bluuebunny/crossref_metadata_2025_split huggingface dataset:
HDAC4 regulates GR signaling Contributes to Stress-Induced Hyperalgesia in the Medial Prefrontal Cortex
Authors: Zhang Li , Chen Chen , Qi Jian 
Published: November 2019

Abstract
“Stress-induced hyperalgesia (SIH)” is a phenomenon that stress can lead to an increase in pain sensitivity. Recent findings showed that epigenetic mechanisms have been known to play fundamental roles in stress and pain. Histone acetylation is an outstanding epigenetic feature that is changed in numerous stress-related disease situations. However, epigenetic mechanism for the control of SIH is not well known. We investigated the effect of histone acetylationon pain hypersensitivity on SPS (single-prolonged stress) + CFA (complete Freund’s adjuvant) model. Here, we show that the glucocorticoid receptor (GR) is regulated by histone deacetylases 4 (HDAC4) on stress-induced hyperalgesia and the paw withdrawal threshold in the SPS+CFA group dropped significantly compared with the control group. HDAC4-expressing neurons in the medial prefrontal cortex (mPFC) were increased in the SPS+CFA-exposed group compared with CFA-exposed group. Inhibiting HDAC4 by microinjection of sodium butyrate into the mPFC could disrupting glucocorticoid receptor (GR) signaling pathway, lowered SPS+CFA-caused mechanical allodynia and alleviated anxiety-like behavior. Together, our studies suggest that HDAC inhibitors may involve in the process of stress-induced anxiety-like behavior, amplifying the sensitivity to pain.
Transcriptomic analysis among sub-clinically ill cattle following Bovine Respiratory Disease vaccine protocol and challenge with Bovine Viral Diarrhea Virus
Authors: Fleming Damarius S , Herring Andy D. , Miller Laura C , Gill Clare A. 
Published: November 2019

Abstract
Background: Bovine viral diarrhea (BVD) is a widespread immunosuppressive disease of cattle. Infection with bovine viral diarrhea virus (BVDV) can also be sub-clinical and persistent in herds, leading to high economic impact to cattle producers. Complicating this is BVD’s ability to predispose cattle to bovine respiratory disease (BRD) complex. Although many producers vaccinate for BRD to help protect herds from this predisposition, there are gaps in the knowledge of differences between the protection afforded by modified live (MLV) or killed (KV) vaccines, and the level of cross-protection they offer to BVDV. These knowledge gaps affect the economic impact of BVDV because, with more information on vaccine efficacy, cattle producers could possibly reduce treatment costs and disease spread. The study objective was to determine the level of BRD vaccine cross-protection by examining the underlying differential gene expression response to a sub-clinical BVDV challenge in Nellore-Angus cattle.
Results: Statistical analysis of clinical data showed that MLV had lower BVDVa, BVDV2, and BVDVb antibody titers (p < 0.001) compared to the KV vaccine. The three treatment groups (no vaccine (NON), KV, MLV; n = 5 per group) were also compared by RNA-seq analysis of buffy coats from cattle at 14 dpi. The BRD KV group displayed a higher antibody (AB) response, however transcriptomic analysis showed that the KV group still showed high innate gene expression at 14 dpi. The MLV showed displayed a higher number of adaptive and cell-mediated immune response genes being differentially expressed in reaction to challenge at 14 dpi. Analysis also showed the difference in vaccine cross-protection involved evidence of complement activation as part of the MLV bolstered immune response. Lack of detection of complement activation in the KV group could explain the higher titers, while complement activation in the MLV group could lead to less AB response due to better cell-mediated response. Upregulation of MHC molecules and T-cell related gene expression was also observed within the MLV group only.
Conclusions: Although both vaccines provide some level of cross-protection over non-vaccinated animals, the results point to MLV producing cross-protection that extends the adaptive immune response to BVDV.
Promoting rational antibiotics prescribing for non-complicated infections: Understanding social influence in primary care networks in Germany
Authors: Poss-Doering Regina , Kamradt Martina , Glassen Katharina , Andres Edith , Kaufmann-Kolle Petra , Wensing Michel 
Published: November 2019

Abstract
Primary care networks in Germany are formalized regional collaborations of physicians and other healthcare providers. Common goals are optimized healthcare processes and services for patients, enhanced communication, agency for professional concerns and strengthened economic power. In the ARena study (Sustainable reduction of antibiotic-induced antimicrobial resistance), 14 primary care networks in two federal German states aimed to promote appropriate antibiotics use for acute non-complicated infections by fostering awareness and understanding. Factors related to the role of primary care networks were to be identified. Methods For this study, audio-recorded telephone interviews were conducted with physicians, non-physician health professionals and stakeholder representatives. Pseudonymized verbatim transcripts were coded using thematic analysis. In-depth analysis was based on the inductive categories ‘social support’, ‘social learning’, ‘social normative pressures’ and ‘social contagion’ to reflect social influence processes. To foster understanding of the networks’ potential impact on antibiotics prescribing, data generated through surveys with physicians and non-physician health professionals were analyzed descriptively. Results : Social influence processes proved to be relevant regarding knowledge transfer, manifestation of best-practice care and self-reflection. Peer communication was seen as great asset, main reason for membership and affirmative for own perspectives. All interviewed physicians (n=27) considered their network to be a strong support factor for daily routines, introduction of new routines, and continuity of care. They utilized network-offered training programs focusing on best practice guideline-oriented use of antibiotics and considered their networks supportive in dealing with patient expectations. A shared attitude combined with ARena intervention components facilitated reflected management of antibiotics prescribing. Non-physician health professionals (n=11) also valued network peer exchange. They assumed their employers joined networks to offer improved and continuous care. Stakeholders (n=7) expected networks and their members to be drivers for care optimization. Conclusion: Primary care networks play a crucial role in providing a platform for professional peer exchange, social support and reassurance. With regards to their impact on antibiotics prescribing for acute non-complicated infections, networks seem to facilitate and amplify quality improvement programs by providing a platform for refreshing awareness, knowledge and self-reflection among care providers. They are well suited to promote a rational use of antibiotics. .
Serum metabolomics study of end-stage renal disease with depression: potential biomarkers for diagnosis and promising targets for therapy
Authors: Hu Jie , Yuan Dezhi , Tian Kuan , Feng Liping , Zhao Qiuye , Liao Xiaohui , Luo Huirong , Jiang Qihui , Wang Xuefei , Zhang Xiaotian , Hu Ling , Wang Hongkai , Zhou Peng , Guo Xingxian , Ran Jianhua , Li Jinfang 
Published: November 2019

Abstract
Background End-stage renal disease (ESRD) is the most severe stage during the development of the renal failure. And depression is the most common psychological disorder in patients with ESRD, which in turn aggravates the progression of renal failure and seriously reduce the quality of life in ESRD patients with depression, but its underlying mechanism remains unclear. This study aimed to reveal the pathogenesis and discover novel peripheral biomarkers for ESRD with depression through metabolomics analysis.Methods Ultra-high-performance liquid chromatography tandem quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS) was used to explore changes of serum metabolites among healthy controls (n = 12), ESRD patients (n = 17), and ESRD patients with depression (n = 17). Also, the differential metabolites between groups were subjected to clustering analysis, pathway analysis, receiver operating characteristic (ROC) curve analysis.Results A total of 57 significant serum differential metabolites were identified between the ESRD without depression group and the ESRD with depression group, which were involved in 19 metabolic pathways, such as energy metabolism, glycerolipid metabolism and glutamate-centered metabolism. Moreover, the area under the ROC curve of Gentisic acid, Uric acid, 5-HT, 2-Phosphoglyceric acid, Leucyl-phenylalanine, Propenoyl carnitine, Malaoxon, Pregnenolone, 6-Thioxanthine 5'-monophosphate, Hydroxyl ansoprazole, Zileuton O-glucuronide, Cabergoline, PA (16:0/18:2(9Z,12Z)), PG (18:0/18:1(11Z)), probucol, etc. and their combination was greater than 0.90.Conclusions Inflammation, oxidative stress and metabolic abnormalities in energy metabolism, glycerolipid metabolism and glutamate-centered metabolism may be associated with the pathogenesis of ESRD with depression, which may be promising targets for therapy. Furthermore, the identified differential metabolites may serve as biomarkers for the diagnosis of ESRD patients with depression.
The Correlation among English Learning Anxiety, Speaking and Writing Achievements of Senior High School Students
Authors: Lestari Dian Eva , Loeneto Bambang , Ihsan Diemroh 
Published: November 2019

The objectives of the study were to analyze whether or not there were a significant correlation among English learning anxiety, speaking, and writing achievements of the eleventh graders’. The sample of the study were 112 eleventh graders’. They were selected randomly. The study used quantitative research design. The questionnaire and the writing and speaking tests were done to analyze the data. The researcher used foreign language classroom anxiety scale questionnaire to measure the students’ English learning anxiety with five-point likert scale, and speaking and writing tests were used to measure the students’ speaking and writing achievements. Pearson product moment correlation and regression were analyzed by using SPSS. The results of study were negative low correlation between English learning anxiety and speaking achievement (r=-.269 with p<0.01) and English learning anxiety and writing achievement (r=-.213 with p<0.05).
Saintpaulia Sulu Yaprak Özütü Kullanılarak Sentezlenen Gümüş Nanopartiküllerin Antibakteriyel ve Antibiyofilm Aktivitesi
Authors: KORKMAZ Nesrin 
Published: November 2019

Günümüzde nanopartiküller farklı alanlarda etkin bir şekilde kullanılmaktadır. İlk zamanlar nanopartiküllerin sentezinde fiziksel ve kimyasal yöntemler kullanılmaktaydı. Kimyasal olarak sentezlenmiş nanoparçacıkların toksik etkisinin üstesinden gelmek için biyosentez (yeşil sentez) bir alternatif olarak ortaya çıkmıştır. Bu çalışmada, Afrika menekşesinin (Saintpaulia) yaprak özütü ile gümüş nanopartiküllerin (AgNP’lerin) yeşil sentezi gerçekleştirilerek karakterizasyonu yapılmıştır. Karakterizasyon sonucunda ~432 nm dalga boyunda spektrum gösteren, 40.4 nm büyüklüğünde küresel boyutta nanopartiküllerin oluştuğu belirlenmiştir. AgNP’lerin dört Gram-pozitif ve dört Gram-negatif bakteri suşlarına karşı antibakteriyel ve biyofilm inhibisyon aktiviteleri belirlenmiştir. 10 mM konsantrasyonda gümüş nanopartiküller tüm bakteri suşlarına karşı bakteriyosidal etki göstermiştir. Antibiyofilm aktivitesi çalışmasında ise en yüksek inhibisyon yüzdesi 10 mM konsantrasyonda %80.3 oranında Salmonella infantis suşuna karşı elde edilmiştir.
A resilience assessment framework for critical infrastructure networks' interdependencies
Authors: Imani Maryam , Hajializadeh Donya 
Published: November 2019

Abstract
Critical infrastructures (CIs) provide essential services to the society. As infrastructures are becoming more interdependent, there is an increasing need for better management of their interactions and interdependencies. Interdependencies among CI can cause cascading failures and, hence, amplify negative consequences due to these failures. This can also affect CIs' service restoration rate and consequently reduce their resilience in coping with these hazardous events. The common challenge currently faced by CI asset owners is the lack of robust resilience-informed business planning and management strategies in response to interdependent assets' failures due to low-probability/high-impact hazards. This is of particular importance as CI owners and managers are investing more on improving the resilience of their assets in response to extreme environmental hazards. This study has approached CI nexus from the interdependency management point of view. It has developed an integrated resilience assessment framework to identify and map interdependency-induced vulnerabilities in critical infrastructure networks. This framework can potentially support effective management of the interdependencies in CI networks. The findings have been reflected in mapping the connection between the changes in resilience due to interdependency-induced failures and the cost of intervention scenarios, providing means of exploring shared intervention strategies.
Enhancing the Therapeutic Efficacy of Bortezomib in Cancer Therapy Using Polymeric Nanostructures
Authors: Korani Mitra , Korani Shahla , Zendehdel Elham , Nikpoor Amin Reza , Jaafari Mahmoud Reza , Orafai Hossein M. , Johnston Thomas P. , Sahebkar Amirhossein 
Published: November 2019

:
Bortezomib (VELCADE®) is a boronate peptide and first-in-class proteasome inhibitor serving an
important role in degenerating several intracellular proteins. It is a reversible inhibitor of the 26S proteasome,
with antitumor activity and antiproliferative properties. This agent principally exerts its antineoplastic effects by
inhibiting key players in the nuclear factor κB (NFκB) pathway involved in cell proliferation, apoptosis, and
angiogenesis. This medication is used in the management of multiple myeloma. However, more recently, it has
been used as a therapeutic option for mantle cell lymphoma. While promising, bortezomib has limited clinical
applications due to its adverse effects (e.g., hematotoxicity and peripheral neuropathy) and low effectiveness in
solid tumors resulting from its poor penetration into such masses and suboptimal pharmacokinetic parameters.
Other limitations to bortezomib include its low chemical stability and bioavailability, which can be overcome by
using nanoparticles for its delivery. Nanoparticle delivery systems can facilitate the targeted delivery of chemotherapeutic
agents in high doses to the target site, while sparing healthy tissues. Therefore, this drug delivery
system has provided a solution to circumvent the limitations faced with the delivery of traditional cancer chemotherapeutic
agents. Our aim in this review was to describe polymer-based nanocarriers that can be used for the
delivery of bortezomib in cancer chemotherapy.
Evaluation of Cell-detaching Effect of EDTA in Combination with Oxaliplatin for a Possible Application in HIPEC After Cytoreductive Surgery: A Preliminary in-vitro Study
Authors: Schubert Justyna , Khosrawipour Tanja , Pigazzi Alessio , Kulas Joanna , Bania Jacek , Migdal Pawel , Arafkas Mohamed , Khosrawipour Veria 
Published: November 2019

Background:
Ethylenediaminetetraacetic acid (EDTA), a commonly used compound in laboratory
medicine, is known for its membrane-destabilization capacity and cell-detaching effect. This preliminary study
aims to assess the potential of EDTA in removing residual tumor cell clusters. Using an in-vitro model, this effect
is then compared to the cytotoxic effect of oxaliplatin which is routinely administered during HIPEC procedures.
The overall cell toxicity and cell detaching effects of EDTA are compared to those of Oxaliplatin and the additive
effect is quantified.

Methods:
HT-29 (ATCC® HTB-38™) cells were treated with A) EDTA only B) Oxaliplatin only and C) both
agents using an in-vitro model. Cytotoxicity and cell detachment following EDTA application were measured via
colorimetric MTS assay. Additionally, detached cell groups were visualized using light microscopy and further
analyzed by means of electron microscopy.

Results:
When solely applied, EDTA does not exhibit any cell toxicity nor does it add any toxicity to oxaliplatin.
However, EDTA enhances the detachment of adherent colon carcinoma cells by removing up to 65% (p<0.05) of
the total initial cell amount. In comparison, the sole application of highly concentrated oxaliplatin induced cell
mortality by up to 66% (p<0.05). While detached cells showed no mortality after EDTA treatment, cell clusters
exhibited a decreased amount of extracellular and adhesive matrix in-between cells. When combined, Oxaliplatin
and EDTA display a significant additive effect with only 30% (mean p <0.01) of residual vitality detected in the
initial well. EDTA and Oxaliplatin remove up to 81% (p <0.01) of adhesive HT-29 cells from the surface either
by cytotoxic effects or cell detachment.

Conclusions:
Our data support EDTA’s potential to remove microscopical tumor cell clusters from the peritoneum
and possibly act as a supplementary agent in HIPEC procedures with chemotherapy. While adding EDTA to
HIPEC procedures may significantly decrease the risk of PM recurrence, further in-vivo and clinical trials are
required to evaluate this effect.
Relationship between Health Costs and Inflammatory Profile in Public Health
Authors: Rocha Ana P.R. , Lira Fábio S. , Bueno Denise R. , Inoue Daniela S. , Queiroz Dayane C. , Codogno Jamile S. 
Published: November 2019

Introduction:
The association between obesity and physical activity level is well established in the literature, as well as its consequences that lead to chronic noncommunicable diseases. In addition, it is also possible to obtain the immunometabolic mechanism that explains the pathway of associations between obesity, chronic noncommunicable diseases and level of physical activity. It also seems clear that treating illnesses has a financial impact on healthcare systems around the world, so it seems important to assess the financial impact on the healthcare system of individuals with immunometabolic dysfunction.

Aim:
To assess whether there is a correlation between metabolic and inflammatory markers and healthcare costs according to body adiposity and habitual physical activity (HPA).

Methods:
cross-sectional study, the sample consisted of men and women aged over 50. Participants underwent evaluations that included the following variables: i) immunometabolic markers, ii) healthcare costs, iii) obesity, iv) habitual physical activity, and v) history of personal illness. Statistical significance was set at values lower than 5% and the software used was BioEstat.

Results:
The correlation between metabolic and inflammatory markers and healthcare costs demonstrated a positive and significant relationship, adjusted for obesity and HPA, between glucose concentrations and exam costs (r = 0.343, p-value = 0.007) and total cost (r = 261; p-value = 0.043); HOMA index and cost of exams (r = 0.267; p-value = 0.038); and IL-10 and cost of medical consultation (r = 0.297; p-value = 0.020).

Conclusion:
Metabolic and inflammatory markers may be related to the costs of consultations and examinations, independent of obesity and HPA.
Exploration of the Hepatoprotective Effect and Mechanism of Swertia mussotii Franch in an Acute Liver Injury Rat Model
Authors: Yun Haixia , Duan Xianglan , Xiong Wendou , Ding Yiwei , Wu Xinyu , Kang Junhua , Pu Xiaoyan , Yang Yingzhong , Chen Zhi 
Published: November 2019

Background and Objective:
Swertia mussotii Franch, also known as “Zangyinchen”, is
one of a Tibetan traditional herb used for treatment of liver diseases over thousands of years at
Qinghai-Tibet Plateau, has been confirmed to be hepatoprotective. However, the underlying
mechanism is largely unknown.

Materials and Method:
In this study, we evaluated the effect of S. mussotii treatment in a carbon
tetrachloride-induced acute liver injury rat model by examining the serum alanine
aminotransferase, aspartate aminotransferase, total bilirubin levels and performing histological
observations of the liver tissues. Meanwhile, the metabolomics analysis was used to explore the
molecular mechanism of S. mussotii treatment by high performance liquid chromatography tandem
mass spectrometry.

Results:
The results showed that S. mussotii treatment could effectively improve the serum alanine
aminotransferase, aspartate aminotransferase, total bilirubin in acute liver injury rat model.
Histological observation showed that S. mussotii treatment could effectively alleviate liver injury.
Moreover, the metabolomics analysis showed that S. mussotii treatment could normalize the levels
of many fatty acid metabolism related metabolites. And the results of pathway analysis showed
that these metabolites significantly enriched in fatty acid biosynthesis pathway (myristic acid,
dodecanoic acid and capric acid) and linoleic acid metabolism pathway (13-OxoODE).

Conclusion:
The results indicated that S. mussotii treatment could significantly improve acute liver
injury through affecting the pathways related to lipid metabolism.
Effects of Hydrogen-rich Water on the PI3K/AKT Signaling Pathway in Rats with Myocardial Ischemia-reperfusion Injury
Authors: Li Liangtong , Li Xiangzi , Zhang Zhe , Liu Li , Liu Tongtong , Li Shaochun , Liu Sen , Zhou Yujuan , Liu Fulin 
Published: November 2019

Background:
The effects of hydrogen-rich water on PI3K/AKT-mediated
apoptosis were studied in rats subjected to myocardial ischemia-reperfusion injury
(MIRI).

Methdos:
Sixty rats were divided randomly into a hydrogen-rich water group and a
control group. The hearts were removed and fixed in a Langendorff device. Hearts from
the control group were perfused with K-R solution, and hearts from the hydrogen-rich
water group was perfused with K-R solution + hydrogen-rich water. The two treatment
groups were then divided randomly into pre-ischemic period, ischemic period and
reperfusion period groups(10 rats per group), which were subjected to reverse perfusion
for 10 min, normal treatment for 20 min, and reperfusion for 20 min, respectively. The
mRNA and protein expression levels of PI3K, AKT, p-AKT, FoxO1, Bim and Caspase-3
in each group were detected by RT-qPCR, immunohistochemistry (IHC) and Western
blotting. Caspase-3 activity was detected by spectrophotometry.

Results:
Among the hydrogen-rich water group, the PI3K/AKT signaling pathway was
significantly activated, and FoxO1, Bim, and Caspase-3 mRNA and protein levels were
significantly decreased in ischemia-reperfusion subgroup compared with the preischemic
and ischemic subgroups. In the ischemia-reperfusion hydrogen-rich water
group, PI3K, AKT and p-AKT mRNA and protein expression levels were increased while
the FoxO1, Bim and Caspase-3 expression levels were significantly decreased
compared with those in the corresponding control group (p<0.05).

Conclusion:
Hydrogen-rich water can activate the PI3K/AKT signaling pathway,
alleviate ischemia-reperfusion injury in isolated rat hearts, and inhibit cardiomyocyte
apoptosis.