bluuebunny/crossref_metadata_2025_split huggingface dataset:
Structural insight into the putative role of novel SARS CoV-2 E protein in viral infection: a potential target for LAV development and therapeutic strategies
Authors: Sarkar Manish , Saha Soham 
Published: May 2020

AbstractThe outbreak of COVID-19 across the world has posed unprecedented and global challenges on multiple fronts. Most of the vaccine and drug development has focused on the spike proteins and viral RNA-polymerases. Using the bioinformatics and structural modeling approach, we modeled the structure of the envelope (E)-protein of novel SARS-CoV-2. The E-protein of this virus shares sequence similarity with that of SARS-CoV-1, and is highly conserved in the N-terminal regions. Incidentally, compared to spike proteins, E proteins demonstrate lower disparity and mutability among the isolated sequences. Using homology modeling, we found that the most favorable structure could function as a gated proton channel. Combining pocket estimation and docking with water, we determined that GLU 8 and ASN 15 in the N-terminal region were in close proximity to form H-bonds. Additionally, two distinct “core” structures were visible, the hydrophobic core and the central core, which may regulate the opening/closing of the channel. We propose this as a mechanism of viral proton channeling activity which may play a critical role in viral infection. In addition, it provides a structural basis and additional avenues for LAV development and generating therapeutic interventions against the virus.Significance StatementStructural modeling of the novel SARS-CoV-2 envelope protein (E-protein) demonstrating its possible proton channeling activity
Nicotinamide mononucleotide redox cofactor system enables aldehyde accumulation inEscherichia coli
Authors: Richardson Kelly N. , Black William B. , Li Han 
Published: May 2020

ABSTRACTIt is challenging to biosynthesize industrially important aldehydes, which are readily consumed by the numerous alcohol dehydrogenases (ADHs) in cells. In this work, we demonstrate that a nicotinamide mononucleotide (NMN+)-dependent redox cofactor cycling system enables aldehyde accumulation inEscherichia colicrude lysates and whole cells. By specifically delivering reducing power to a recombinant enoate reductase, but not to endogenous ADHs, we convert citral to citronellal with minimal byproduct formation (98% and 83% product purity in crude lysate- and whole cell-based biotransformation, respectively). We envision the system’s universal application to lower the noise in biomanufacturing by silencing the host’s metabolic background.
Piggybacking on the Cholera Toxin: Identification of a Toxoid-binding Protein as an Approach for Targeted Delivery of Proteins to Motor Neurons
Authors: Balmforth Matthew R. , Haigh Jessica , Tiede Christian , Tomlinson Darren C. , Deuchars Jim , Webb Michael E. , Turnbull W. Bruce 
Published: May 2020

ABSTRACTA significant unmet need exists for the delivery of biologic drugs such as polypeptides or nucleic acids, to the central nervous system (CNS) for the treatment and understanding of neurodegenerative diseases. Naturally occurring toxoids have been considered as tools to meet this need. However, due to the complexity of tethering macromolecular drugs to toxins, and the inherent dangers of working with large quantities of recombinant toxin, no such route has been successfully exploited. Developing a method where toxoid and drug can be assembled immediately prior toin vivoadministration has the potential to circumvent some of these issues. Using a phage-display screen, we identified two antibody mimetics, Anti-Cholera Toxoid Affimer (ACTA) -A2 and ACTA-C6 that non-covalently associate with the non-binding face of the cholera toxin B-subunit. In a first step toward the development of a non-viral motor neuron drug-delivery vehicle, we show that Affimers can be selectively delivered to motor neuronsin vivo.
Cluster role in industry 4.0 – a pilot study from Germany
Authors: Götz Marta 
Published: May 2020

PurposeThis paper aims to explore the nature of Industry 4.0 (I4.0) cluster, to establish and empirically verify in the pilot study the role of clusters in developing the fourth industrial revolution. It aims to find out if the cluster can provide a conducive knowledge environment fostering the advancement of I4.0; simplify the implementation of I4.0 by making it faster, easier, and cheaper and finally be applied as policy tool organising the development of I4.0.Design/methodology/approachThis paper is based on the narrative literature review mapping the cluster’s nature with I4.0 features. It adopts the case study approach and uses simple statistical analysis to explore the basic characteristics of I4.0 clusters combined with a survey technique – short questionnaire organised in three major blocks.FindingsDrawing on German pilot study it can be confirmed that clusters offer conducive environment facilitating the emergence, testing and development of I4.0 specific solutions. They provide favourable knowledge environment, simplify and increase the efficiency of the business processes and organise the policymaking in this area.Originality/valueThe results of presented pilot study rooted in Germany – a country seen as the front-runner in implementing the solutions of the fourth industrial revolution – can add a certain value to the emerging research on unearthed linkages between clusters and I4.0. This paper might be seen as a contribution to the emerging literature on the spatial dimension of I4.0. It expands previous research on cluster channels’ likely impact on I4.0.
A tipping point analysis of service-learning hours and student outcomes
Authors: Garger John , Vracheva Veselina P. , Jacques Paul 
Published: May 2020

PurposeAlthough extant literature links overstimulation to various job outcomes, most studies do not consider a service-learning context, and they suggest a linear association between stimuli and outcomes. This paper examines the link between the number of service-learning hours students work and three educational outcomes – student satisfaction with the service-learning project, class relevancy to the service-learning project and expected community involvement.Design/methodology/approachApplying activation theory and Yerkes–Dodson law, we test curvilinear relationships between service-learning hours and student outcomes.FindingsResults suggest that students benefit from service learning up to a certain duration of a service-learning project.Originality/valueThis study identifies the tipping point of the number of service-learning hours beyond which students perceive decrements to three outcomes.
Georgian higher education from the perspective of the international literature from 2003 to 2019
Authors: Lanahan Brian 
Published: May 2020

PurposeHigher education in the Republic of Georgia has experienced a massive isomorphic transformation since the Rose Revolution of 2003 and continues to transform with aspirations toward Euro-Atlantic integration, compliance with the Bologna Process and as a reflection of the Europeanization of its higher education sector.Design/methodology/approachThis literature review documents and analyzes literature published between 1991 and 2019 on the development of higher education in the Republic of Georgia from 2003 to 2019.FindingsThe findings reflect the evolving political landscape and aspiration for Euro-Atlantic integration against the backdrop of one of the most impoverished education systems in the world, as measured by percentage of gross domestic product spending. More explicitly, what findings have been reported in the international literature on Georgian higher education from 2003 to 2019?Research limitations/implicationsThe choice to review only English publications was deliberate and done after consultation with a leading Georgian scholar, who noted that the Georgian language publishing market is small and of varied quality; leading Georgian scholars most often seek to publish in English journals and books, and all technical and policy reports produced by the Minister of Education, NGOs and large international organizations (e.g. World Bank) are available in English.Originality/valueThis literature review documents and analyzes literature on the development of higher education in the Republic of Georgia from 2003 to 2019 as a reflection of the evolving political landscape and aspiration for Euro-Atlantic integration against the backdrop of one of the most impoverished education systems in the world, as measured by percentage of gross domestic product (GDP) spending. This unique political and economic history makes higher education in Georgia worthy of review.
When is transparent packaging beneficial?
Authors: Sabri Ouidade , Doan Hai Van , Malek Faten , Bachouche Hager 
Published: May 2020

PurposeThe purpose of this paper is to demonstrate that the positive effect of packaging transparency on purchase intention is moderated by product quality risk (PQR) associated with the product category.Design/methodology/approachTwo separate experiments were conducted. Study 1 was designed to test the mediating role of perceived quality to account for the positive effect of transparency on purchase intention. Two types of packaging (opaque vs transparent) for a product associated with a high level of PQR were examined. Study 2 extended the findings by introducing the moderating role of PQR. A 2 (type of packaging: opaque vs transparent)*2 (PQR: low vs high) between subjects design was used.FindingsThe moderating role of the product PQR level is established: transparent packaging improves the product perceived quality and brand purchase intention when the product is associated with a high PQR, whereas there is no such preference for transparent packaging when the product is associated with a low PQR.Practical implicationsThe results offer insights to better understand the potential gains from adopting transparent packaging. If a brand manager's main goals are to develop sales, costly investments in research and development of transparent packaging appear to be fruitful only for products associated with high PQR.Originality/valueThis paper contributes to packaging, cue utilisation and perceived risk literatures by evidencing the moderating role of PQR to explain the positive effect of transparency on purchase intention.
Hybrid calibration and detection approach for mobile robotic manufacturing systems
Authors: Bai Jing , Zhang Yuchang , Qin Xiansheng , Wang Zhanxi , Zheng Chen 
Published: May 2020

PurposeThe purpose of this paper is to present a visual detection approach to predict the poses of target objects placed in arbitrary positions before completing the corresponding tasks in mobile robotic manufacturing systems.Design/methodology/approachA hybrid visual detection approach that combines monocular vision and laser ranging is proposed based on an eye-in-hand vision system. The laser displacement sensor is adopted to achieve normal alignment for an arbitrary plane and obtain depth information. The monocular camera measures the two-dimensional image information. In addition, a robot hand-eye relationship calibration method is presented in this paper.FindingsFirst, a hybrid visual detection approach for mobile robotic manufacturing systems is proposed. This detection approach is based on an eye-in-hand vision system consisting of one monocular camera and three laser displacement sensors and it can achieve normal alignment for an arbitrary plane and spatial positioning of the workpiece. Second, based on this vision system, a robot hand-eye relationship calibration method is presented and it was successfully applied to a mobile robotic manufacturing system designed by the authors’ team. As a result, the relationship between the workpiece coordinate system and the end-effector coordinate system could be established accurately.Practical implicationsThis approach can quickly and accurately establish the relationship between the coordinate system of the workpiece and that of the end-effector. The normal alignment accuracy of the hand-eye vision system was less than 0.5° and the spatial positioning accuracy could reach 0.5 mm.Originality/valueThis approach can achieve normal alignment for arbitrary planes and spatial positioning of the workpiece and it can quickly establish the pose relationship between the workpiece and end-effector coordinate systems. Moreover, the proposed approach can significantly improve the work efficiency, flexibility and intelligence of mobile robotic manufacturing systems.
Understanding regional variation in fish prices: evidence from a developing economy
Authors: Rajeev Meenakshi , Nagendran Pranav 
Published: May 2020

PurposeThis paper examines the prices of fishery products (an important source of protein for the poor) and drivers of their inter-regional variations in India, where fishery is a critical sector. By explaining regional price differences, we make an attempt to derive policy implications as to how fish price inflation can be controlled.Design/methodology/approachThe paper is primarily based on secondary data provided by the Ministry of Agriculture, Government of India. In the absence of data on inter-regional trade, appropriate indicators are constructed using the gravity model to capture supply side factors that may influence regional price differences. Pooled regressions are carried out for a representative marine and an aquaculture fish variety separately for the period 2011 to 2017.FindingsAfter controlling for income levels, it is found that marine fish prices can be reduced by improving intra-state transport infrastructure. For reducing the price of aquacultures, it is shown that it is imperative to reduce the distance between producers and consumers.Research limitations/implicationsThe study is limited by the availability of data on interstate trade and consumption of fish and has only used prices of representative fish varieties instead of average marine and aquaculture fish prices.Originality/valueThis paper considers trade and value chain based business theories to explain regional price differences. It analyzes the drivers of relative price differences and suggests measures to control them using a gravity model of trade.
Co-morbid psychological distress groups among incarcerated men
Authors: Wolff Nancy , Caravaca Sánchez Francisco 
Published: May 2020

Purpose
This study aims to examine the behavioral health disorders and trauma exposure are disproportionately represented among incarcerated men. Historically, prisons have been inadequately equipped to respond to the behavioral health needs of incarcerated people. Given the abundance of behavioral health need and the relatively limited availability of prison-based treatment resources, population health management strategies, particularly need stratification, are vital.

Design/methodology/approach
A sample of 943 male inmates from three Spanish prisons completed a structured questionnaire. Need groups are based on current depression, anxiety and stress symptoms assessed by the DASS-21 and were validated using adverse childhood experiences (ACE), prison-based abuse, prison-based substance use, social support and resilience.

Findings
Three need groups were identified, namely, minimal, mild/moderate and severe, each representing about one-third of the sample. The severe group had the highest level of all three types of psychological distress, ACE and prison-based adversity and substance use. No statistical differences in social support and resilience were found among the groups. These findings provide a platform for future research to explore how the complexity of behavioral health care need can be identified and stratified for strategic and rational treatment matching. Proving whether a population health management approach improves behavioral health and personal safety outcomes within funding-constrained carceral environments is the next research priority.

Originality/value
This study is the first to group co-morbid psychological distress into need categories using a social determinants of health framework for validation.
Manufacturing network integration and culture: an institution-based view
Authors: Arellano Maricela C. , Sancha Cristina , Netland Torbjørn , Gimenez Thomsen Cristina 
Published: May 2020

PurposeIn pursuit of increased competitiveness, global manufacturers often seek tighter integration among the plants in their production networks. However, this is a challenging task because plants are dispersed across multiple institutional environments. Although the literature provides abundant evidence of how formal institutional environments affect the integration among plants, little is known about the role of the informal institutional environment – such as culture. In this study, the authors investigate the relationship between different dimensions of culture and manufacturing network integration.Design/methodology/approachThe authors combine survey data from the most recent International Manufacturing Strategy Survey with secondary data that capture cultural dimensions. They then analyze the responses from 581 assembly plants in 21 countries obtained from the survey using a multilevel regression model.FindingsThe study results show that plants located in masculine and long-term-oriented national cultures are associated with lower levels of integration with other plants. The results for the other four Hofstede dimensions of national culture were not statistically significant. At the level of organizational culture, the authors found that a collaborative plant environment positively relates to higher levels of network integration. They did not find statistically significant evidence for the relationship between cultural or geographical distance and network integration.Practical implicationsThis research provides managers with practical insights into the types and combinations of cultural environments that affect the integration of plants in a global network. This knowledge is useful for informing effective integration strategies and tactics.OriginalityThe authors provide new, empirical evidence of the relation between the informal institutional environments of a plant and its integration in a manufacturing network. Drawing on an institution-based view, they contribute to the literature on manufacturing networks by discussing and testing empirically the role of national and organizational culture in network integration.
Risk of robbery in a tourist destination: a monthly examination of Atlantic City, New Jersey
Authors: Drawve Grant , Kennedy Leslie W. , Caplan Joel M. , Sarkos James 
Published: May 2020

Purpose
The purpose of this study is to identify potential changes in crime generators and attractors based on monthly models in a high-tourist destination.

Design/methodology/approach
A risk terrain modeling approach was used to assess spatial relationships between 27 crime generator and attractor types in Atlantic City, New Jersey with robbery occurrence for the 2015 calendar year. In total, 12 separate monthly models were run to identify changes in risk factors based on the month of the year.

Findings
Results indicated unique significant risk factors based on the month of the year. Over the warmer and summer months, there was a shift in environmental risk factors that falls in line with more of a change in routine activities for residents and tourists and related situational contexts for the crime.

Practical implications
The analytical approach used in the current study could be used by police departments and jurisdictions to understand types of crime generators and attractors influencing local crime occurrence. Subsequent analyses were used by Atlantic City Police Department to direct place-based policing efforts.

Originality/value
With growing crime and place research that accounts for temporal scales, the authors advance these endeavors by focusing on a tourist destination, Atlantic City, New Jersey.
The effect of nepotism on tolerance to workplace incivility: mediating role of psychological contract violation and moderating role of authentic leadership
Authors: arici Hasan Evrim , Arasli Huseyin , Cakmakoglu Arici Nagihan 
Published: May 2020

PurposeThis multilevel study investigates the effect of employees' perception of nepotism on tolerance to workplace incivility through the mediating role of psychological contract violation and the moderating role of authentic leadership in organizations.Design/methodology/approachUsing time-lagged data from 547 frontline employees working in four- and five-star hotels, this study's hypotheses were analyzed by conducting hierarchical regression analysis and hierarchical linear modelling.FindingsThe findings indicate that non-family members' perception of nepotism triggered perceived tolerance to the uncivil behavior of family members by the management and that this relationship between nepotism perception and tolerance to workplace incivility was mediated by psychological contract violation. In line with expectations, authentic leadership moderated the effect of nepotism perception on tolerance to workplace incivility.Originality/valueThis study is among the first to examine the effects of nepotism perception on tolerance to workplace incivility by focusing on the mediator role of psychological contract violation at the individual level and the moderator role of authentic leadership at the group level.
Why export competitiveness differs within Indian textile industry? Determinants and empirical evidence
Authors: Dhiman Rahul , Kumar Vinod , Rana Sudhir 
Published: May 2020

Purpose
This study aims to examine whether export competitiveness (EC) in the two groups of the Indian textile industry i.e. “textiles” and “textile products” group differ.

Design/methodology/approach
The study examines how exchange rate (ER), real effective exchange rate (REER) and EC of both the groups are related in the long run over the period 1991-1992 to 2018-2019 using Granger causality test and Johansen and Juselius co-integration test.

Findings
The study confirms that EC is a challenge that needs to be addressed to sustain in the international market, as the volatile trend can be found for EC in both groups. The econometric framework shedding light on both groups of the textile industry suggest that select determinants have different relationships with the EC for two groups. The findings of the Granger causality test reveal that the presence of unidirectional causality running from ER to EC in the case of both the groups. Also, the select variables are found to be co-integrated in the long run. However, in the case of REER, no causality is found running from REER to EC.

Originality/value
ER is a vital determinant of EC and exporters can sustain competitiveness in global markets by reducing their profit mark-up in the face of an appreciating currency.
What do we learn from dystrophic epidermolysis bullosa, nails only? Idiopathic nail dystrophy may harbor a COL7A1 mutation as the underlying cause
Authors: Yang Rui , Duan Yuanyuan , Kong Qingtao , Li Weiwei , Xu Jie , Xia Xinyi , Sang Hong 
Published: May 2020

AbstractDystrophic epidermolysis bullosa (DEB) is a genodermatosis caused by mutations in the COL7A1 gene. DEB, nails only (DEB‐na), is a rare type of DEB. Patients with DEB‐na can be overlooked, and genetic testing is helpful to determine the correct diagnosis. We collected two families with DEB‐na. Clinical information was analyzed. Ultrastructural analysis of the skin tissue was performed. Blood samples were obtained. Next‐generation sequencing was performed and the results were confirmed by Sanger sequencing. A genetic study revealed two novel heterozygous mutations: COL7A1:c.6742G>A (p.G2248R) in patient 1 and c.7181C>G (p.P2394R) in patient 2. Precise diagnosis was made for every patient based on clinical findings and genetic studies. We summarized the phenotype and COL7A1 mutations related to DEB‐na. We report a new phenotype of DEB‐na and two novel mutations in COL7A1. In addition, we emphasize the importance of careful clinical examination and genetic testing in the diagnosis of DEB‐na.