bluuebunny/crossref_metadata_2025_split huggingface dataset:
Analysis of Leaf Trait and Differentially Expressed Genes of Leaf Development in Eucommia ulmoides Oliver after Ultraviolet-B (UV-B) Radiation
Authors: Dewen Li , Ying Liu , Dandan Du , Yuangang Zu , Zhonghua Tang , Yusen Zhao 
Published: October 2020

Abstract
Background Eucommia ulmoides Oliver (EUO) has a great medicinal value in China. The using leaf model of Eucommia planting (ULMEP) is a promising approach to resolve the shortage of EUO resources. We focused on leaf responses and differentially expressed genes (DEGs) for transcriptome profiling after ultraviolet-B radiation (UV-B) in the east-north China. ResultsThe leaf traits were significantly affected after UV-B treatment, and the leaf area, leaf thickness and total leaf fresh/dry weight significantly increased (p<0.05). Anatomical results showed that cells from the leaf epidermal and palisade tissue were larger than those of control. Two transcriptome databases were assembled and integrated, the transcriptome databases comparative analysis revealed 1,826 up-regulated and 1,872 down-regulated genes. Among them, the up-regulated unigenes were mainly participated in Signal transduction mechanisms, and Cell wall/membrane biogenesis by the KOG database, and up-regulated genes were analyzed in plant hormone signal transduction and phenylpropanoid metabolic pathways by KEGG pathway. Furthermore, randomly 10 candidate unigenes expression were examined using RT-qPCR, the two libraries exhibited differential expression and were same as those obtained by sequencing. Conclusions This study was helpful to clarify the cause of leaf enlargement after UV-B treatment, the differentially expressed genes (DEGs) from plant auxin and phenylpropanoid biosynthesis signal pathways were focused on after UV-B treatment. This study provided a valuable reference for the ULMEP in the east-north China.
History of severe hypoglycemia in type II diabetes mellitus unmasked significant atherosclerotic coronary artery disease: a matched case-control study
Authors: Raffali Mohd Asyiq Al-Fard bin Mohd , Muhammad Syawal Faizal , Sukor Norlela , Hamid Hamzaini Abdul , Jyung Tiau Wei , Govindaraju Shathishkumar , Farouk Noor Diyana Mohamad , Nawi Azmawati Mohammed , Hassan Hamat Hamdi Che , Kamaruddin Nor Azmi 
Published: October 2020

Abstract
BackgroundHistory of severe hypoglycemia (SH) has been associated with cardiovascular (CV) events among patients with type II diabetes mellitus (T2DM). In this study, we screened patients with SH for atherosclerotic coronary artery disease (ACAD) .MethodsWe conducted a matched case-control study involving 28 T2DM patients with SH within the last 5 years with no documented ACAD and matched them with 28 T2DM patients with no SH. All subjects underwent coronary artery calcium scoring (CACS) followed by CT coronary angiography (CCTA) if indicated.ResultsHistory of SH in T2DM was associated with 78% (22 out of 28) incidence of significant ACAD compared to 46% (13 out of 28) in those without SH, based on either CACS≥400, or at least one coronary artery with ≥50% stenosis from CCTA, ( P =0.026).ConclusionThis study supported that SH in T2DM is associated with ACAD compared to T2DM without SH.
Laparoscopic Fixation of Biological Mesh at Hiatus with Glue and Suture During Hiatal Hernia Repair
Authors: Nie Yusheng , Yang Huiqi , Xiong Yao , Guan Lei , Yuan Xin , Chen Fuqiang , Chen Jie 
Published: October 2020

Abstract
Aim: Laparoscopic paraesophageal hernia repair is associated with higher recurrence rate. Mesh is used to reduce the recurrence rate. This retrospective study is to review our experience of biological mesh fixed with suture and medical glue in hiatal hernias repairs.Methods: A retrospective chart review was conducted for a consecutive series of patients undergoing laparoscopic hiatal herniorrhaphy between January 2018 and January 2019. After hiatus closure, a piece of biological prosthesis was fixed with medical glue and suture for reinforcement of the crural closure. Clinical outcomes were reviewed, and data were collected regarding operative details, complications, symptoms, and follow-up imaging. Radiological evidence of any size of hiatal hernia was considered to indicate a recurrence.Results: Thirty-six patients underwent surgery uneventfully without any serious complication. There was no mortality. The follow-up was, on average, 18.4 months, and there was no symptomatic recurrence. There was one anatomical recurrence without any related presentation. The method of mesh fixation with medical glue and suture took 12 mins on average, and the handling was fairly easy.Conclusions: Biological mesh fixed with suture and medical glue was safe and effective for repairing large hiatal hernias. Of course, a longer follow-up is still needed for determining long-term outcomes.
Diagnosis and Treatment of Obturator Hernia: Retrospective Analysis of 86 Clinical Cases at a Single Institution.
Authors: LI ZHENGZHENG , Gu Chaoyang , Wei Mingtian , Yuan Xing , Wang Ziqiang 
Published: October 2020

Abstract
Background: To explore the clinical characteristics, diagnosis and treatment of obturator hernia.Methods: Eighty-six patients who were diagnosed as obturator hernia by abdominal CT in the Department of Gastrointestinal Surgery of our hospital between 2009 and 2019 were enrolled in this study. Patient characteristics, surgical method, postoperative complications and mortalities were retrospectively reviewed, and the patients were followed by telephone or clinic visit to check for the recurrence.Results: 30 days mortality rate of 5.5% and 46.1% were observed in surgery group and non-surgery group, respectively. Surgery was performed as an emergency procedure in 59 cases and elective procedure in 14 cases depending on different hernia contents, intestinal necrosis and signs of peritonitis. In the emergency surgery group, segmental intestinal resection with anastomosis was performed in 24 patients(24/59, 40.7%). There were 4 deaths(4/59, 6.8%) in this group ,all of which occurred in patients undergoing SI resections. In contrast, no bowel resection, postoperative complications, or death occurred in the elective surgery group. 3 -year recurrence rates of 5.1% (3/59)and 7.1%(1/14) were observed in the emergency surgery and the elective surgery group, respectively. Conclusions: CT examination plays an important role in improving the diagnostic rate of obturator hernia. In elderly people with comorbidities, timely surgical treatment is the key to improve the efficacy of obturator hernia and prevent the deterioration of the condition. In addition, postoperative mortality is significantly associated with bowel resection and postoperative complications.
Clinical analysis of ECOG PS and adverse reactions in patients with anlotinib at advanced NSCLC as the third or further line treatment: a retrospective observational study
Authors: gu wei guo , Hu MingBin , Deng JianXiong , Qiu Feng 
Published: October 2020

Abstract
Background: Previous studies have shown that anlotinib be a decent choice in third- or futher line treatment of advanced NSCLC. However, the study anslysis of relationship between Eastern Cooperative Oncology Group performance status(ECOG PS) and adverse reactions in advanced NSCLC with anlotinib therapy is less. Methods: We evaluated the efficacy and toxicity of anlotinib in patients with previously treated advanced NSCLC from June 2018 to March 2019. Survival analysis was performed by the Kaplan–Meier method.Results: According to ECOG PS devided into PS 0-1 group(n=63) and PS 2 group(n=28) . The PS 0-1 of median progression-free survival (mPFS, 5.5 vs.2.7 months, P＜0.05) , overall survival (mOS, 9.2 vs.4.7 months，P＜0.05) was longer than PS 2. The PS 0-1 of objective remission rate(ORR, 45.1% vs 6.6%), disease control rate(DCR, 8.8% vs 1.1%) was significanly more than PS 2. The PS 0-1 group of I-II adverse reactions was high than PS 2 group(52.3% vs 32.1%), but the III-IV adverse reactions were less than PS 2 group(9.5% vs 10.7%). Moreover, multivariate analysis indicated that PS was independent risk factor of PFS（HR=2.816, 95%CI：1.661-4.773，P<0.0001）as well as OS(HR=3.188, 95%CI：1.789-5.682，P=0.0001) for anlotinib therapy in NSCLC. Conclusions: Patients in advanced NSCLC with PS 0–1 get better PFS and OS than PS 2 followed by anlotinib treatment in NSCLC. Advanced lung squamous cell carcinoma(LUSC) recived anlotinib treatment with the same efficacy comparable to adenocarcinoma, and patients with PS 0-1 may benefit the most than PS 2.
Absorbable self-retaining barbed double-needle suture(SRBDS) in transperitoneal laparoscopic partial nephrectomy for T1 renal neoplasms
Authors: Wei Hai-bin , Bai Yu-chen , Zhang Qi , Mao Zu-jie , Li En-hui , Wang Shuai , Qi Xiao-long , Liu Feng , Zhang DaHong 
Published: October 2020

Abstract
PurposeThe purpose of study was to evaluate the safety and hemostatic efficacy of absorbable self-retaining barbed double-needle suture (SRBDS) in transperitoneal LPN for T1 renal neoplasms.MethodsIn total, 75 patients with renal neoplasm masses (< 7 cm) undergoing transperitoneal LPN were collected since January 2018 to October 2019. After tumor resection with a cold Endoscissor, 3-0 and 0 SRBDS was performed for inner and outer running stitches without either hem-o-lok or LapraTy clip, respectively. Patient demographic information, neoplasm characteristics, perioperative factors, and oncologic outcomes were collected and analyzed. ResultsAccording to our result, Median tumor size and R.E.N.A.L score was 4.69 cm and 7. Median operative time and overall warm ischemia time was 73 min and 13.14 min. Mean estimated blood loss was 67 ml. intraoperative complications was not showing up and no case was switched to open radical nephrectomy. Postoperative pathological assessment confirmed that negative surgical margins. only single patient experienced secondary surgery-related hemorrhage on the fifth day post-operation, and was treated by transfusion and digital subtraction angiography (DSA) highly selective embolization of the bleeding vessel. No patients developed urinoma or urinary fistula. the overall complications were manageable during three-year follow-up period. ConclusionSRBDS was a safe and efficient surgical endoscopic suturing technique with low complication rate in T1 renal neoplasms, which might be considered as an alternative to other suturing techniques, tissue sealants and glues for partial nephrectomy in the future.
Pancreatic Duct Occlusion: A Safe and Valid Alternative&nbsp;to Duct Anastomosis After Pancreaticoduodenectomy in a Low Volume Centre
Authors: Giuliani Antonio , Rocca Aldo , Segreto Anna Lucia , Paccone Marianna , Izzo Maria Lucia , Buondonno Antonio , Luciani Claudio , Natale Giuseppe Di , Coluzzi Mariagrazia , Komici Klara , Amato Bruno , Calise Fulvio 
Published: October 2020

Abstract
Background: Pancreaticoduodenectomy is the only possible choice of treatment for peri-ampoullar neoplasms. Morbidity in pancreatic surgery is mainly related to the development of a postoperative pancreatic fistula (POPF). According to International Study Group on Pancreatic Fistula it is possible to grade POPF based on clinical variables. Three main different surgical strategies have been proposed to deal with the pancreatic stump following pancreaticoduodenectomy: pancreatojejunostomy, pancreatogastrostomy and pancreatic duct occlusion, but none of them has been clearly demonstrated to be superior to the others. The aim of our study is to evaluate the feasibility of duct occlusion and its correlations with postoperative pancreatic fistula, “brittle diabetes” and overall survival in a low volume centre. We decided to review our previous experience in the light of the recent Covid pandemic where, in our country, it has been forced in many regions to displace treatment of oncological patients in low volume hospitals with limited experienceMethods: We retrospectively reviewed 56 consecutive patients, from a prospective maintained database, who underwent Whipple’s procedure from January 2007 to December 2014 in a tertiary Hepatobiliary Surgery and Liver Transplant Unit with a low volume of pancreatic resections. The mean follow-up was 24.5 months. Results: The overall incidence of postoperative pancreatic fistula was 66.6%: 15 patients had a Grade A (31.25%), 13 a Grade B fistula (27.03%), and 4 (8.3%) suffered from a life-threatening Grade C fistula. At the last follow-up, 24 of the 28 patients who were alive (85.6%) habitually used substitutive pancreatic enzyme. Conclusion: Duct occlusion can be a safe alternative to pancreatic anastomosis especially in low volume centres and for those patients (age >75 years, obese, hard pancreatic texture, small pancreatic duct) at higher risk of clinically relevant POPF.Trial registration: 'retrospectively registered'
Association Between Prenatal Phthalate Exposure and Anthropometric Measures of Newborns in a Sample of Iranian Population
Authors: Darvishmotevalli Mohammad , Moradnia Maryam , Hosseini Reza , Bina Bijan , Feizi Awat , Pour Karim Ebrahim , Pourzamani Hamid Reza , Kelishadi Roya 
Published: October 2020

Abstract
Background: This study aimed to examine the associations between the urinary concentrations of phthalate metabolites among a sample of Iranian pregnant women with anthropometric measures of neonates. Methods: Urine samples were obtained from 121 pregnant women at their first trimester of pregnancy; the levels of monobutyl phthalate (MBP), mono-benzyl phthalate (MBzP), mono-2-ethylhexyl phthalate (MEHP), and mono (2-ethyl-5hydroxyhexyl) phthalate (MEHHP) metabolites were determined by gas chromatography mass spectrometry (GC/MS). The correlation between the urinary concentration of these metabolites and some socio-demographic factors of the participants (maternal education, age, family income, pre-pregnancy body mass index), their lifestyle variables (smoking habit, food pattern, and physical activity), cleaning products use data (cosmetic and household cleaning products) with anthropometric measures of neonates were investigated. Results: MBzP, MBP, MEHP, and MEHHP were detected in 100% of participants with the concentration ranged from 120 to 860 μg/g creatinine. Significant correlations were observed between the urinary levels of maternal MBzP (adjusted β =0.3 (0.001), p=0.03) and MEHHP and (adjusted β =0.3 (0.001), p=0.04) with the birth weight of female neonates. MBP (adjusted β = -0.3(0.02), p=0.04) and MBzP (adjusted β =-0.3 (0.001), p=0.02) were found negatively associated with head circumference in male and female newborns, respectively. Significant differences existed in the birth weight of infants whose mothers used plastic packaging for pickle and those who had history of passive smoking (p-value< 0.05). Conclusions: the studied metabolites had higher concentration in the Iranian pregnant women urine compared to the other countries. Higher levels of prenatal exposure to phthalate metabolites may adversely impact the health status of newborns.
Mental Distress and Associated Factors Among Hospitalized Medical Surgical Adult Inpatients in Public Hospitals, Addis Ababa, Ethiopia, 2020. Cross-sectional Study
Authors: Tesfa Shegaw , Giru Berhanu Wordofa , Bedada Tadesse 
Published: October 2020

Abstract
Background: Mental distress is a mental or psychological syndrome, which influences the health status and treatment effectiveness, getting quality of care in a hospitalized medical surgical inpatient. It is more common in hospital setting than community setting population. Thus, this study aimed to assess the prevalence of mental distress and associated factors among hospitalized medical surgical adult inpatients in public hospitals, Addis Ababa, Ethiopia, 2020.Methods: Institutional based cross-sectional study was conducted with a total of 408 study subjects from March 1-30, 2020. Systematic random sampling technique was used and data was collected using interviewer administered questionnaire. Data was collected by trained nursing students and collected data was entered into Epi-data 3.1 and exported to SPSS version 26 for analysis, and then binary and multiple logistic regressions was performed to check the association between dependent and independent variable.Result: The prevalence of mental distress among hospitalized medical surgical adult inpatients in public hospitals was 53.1% with (95%CI; 48%, 58%). Variables of being married [AOR=2.67; 95%CI(1.065,6.683)], private employee [AOR=2.21; 95%CI(1.001, 4.900)], daily laborer [AOR=4.70; 95%CI(1.218, 18.215)], rural residence [AOR=1.85; 95%CI(1.047,3.264)], taking alcohol [AOR=1.68; 95%CI(1.025, 2.740)], previous psychiatric illness [AOR=3.40; 95%CI(1.078, 10.737)] and co-morbidity [AOR=1.93; 95%CI (1.200, 3.094)] were found to be significantly associated with mental distress; while age, sex, ethnicity, religion, educational status, income, smoking, chat, social support, living condition, history of hospitalization, ward admitted and length of hospital stay were not significantly associated.Conclusion: The prevalence of mental distress was high and being married, private employee, daily laborer, residence in rural area, previous history of psychiatric illness, alcohol used and comorbidity disorder were associated with mental distress among hospitalized adult inpatients. Therefore, health care providers should provide special consideration to those group of patients admitted to the hospital.
Spatio-Temporal Modelling of Culicoides Latreille (Diptera: Ceratopogonidae) Populations on Reunion Island (Indian Ocean)
Authors: Grimaud Yannick René Pierre , Tran Annelise , Benkimoun Samuel , Boucher Floriane , Esnault Olivier , Cêtre-Sossah Catherine , Cardinale Eric , Garros Claire , Guis Hélène 
Published: October 2020

Abstract
Background: Reunion Island regularly faces outbreaks of bluetongue and epizootic hemorrhagic diseases, two insect-borne orbiviral diseases of ruminants. Hematophagous midges of the genus Culicoides (Diptera: Ceratopogonidae) are the vectors of bluetongue (BTV) and epizootic hemorrhagic diseases of deer (EHDV) viruses. In a previous study, statistical models based on environmental and meteorological data were developed for the five Culicoides species present in the island to provide a better understanding of their ecology and predict their presence and abundance. The purpose of this study was to couple these statistical models with a Geographic Information System (GIS) in order to produce dynamic maps of the distribution of Culicoides throughout the island. Methods: Based on meteorological data from ground weather stations and satellite-derived environmental data, the abundance of each Culicoides species was estimated for the 2,214 husbandry locations on the island for the period ranging from February 2016 to June 2018. A large-scale Culicoides sampling campaign including 100 farms was carried out in March 2018 to validate the model. Results: According to the model predictions, no husbandry location was free of Culicoides throughout the study period. The five Culicoides species were present on average in 57.0% of the husbandry locations for C. bolitinos, 40.7% for C. enderleini, 26.5% for C. grahamii, 87.1% for C. imicola and 91.8% for C. kibatiensis. The models also showed high seasonal variations in their distribution. During the validation process, predictions were acceptable for C. bolitinos, C. enderleini and C. kibatiensis, with normalized root mean square errors (NRMSE) of 15.4%, 13.6% and 16.5% respectively. The NRMSE was 27.4% for C. grahamii. For C. imicola, the NRMSE was acceptable (11.9%) considering all husbandry locations except in two specific areas, the Cirque de Salazie – an inner mountainous part of the island- and the sea edge, where the model overestimated its abundance. Conclusions: Our model provides, for the first time, an operational tool to better understand and predict the distribution of Culicoides in Reunion Island. As it predicts a wide spatial distribution of the five Culicoides species throughout the year and taking into consideration their vector competence, our results suggest that BTV and EHDV can circulate continuously on the island. It implies significant limitations for any preventive or control actions targeting vector populations. To assess other control strategies, our model could be coupled with an epidemiological model of BTV and EHDV transmission.
Ultrasound Assisted Extraction and Response Surface Methodology and Artificial Neural Network Optimization of Pectin From Orange Peel
Authors: Hundie Ketema Beyecha 
Published: October 2020

Abstract
The objective of study was ultrasound-assisted extraction of pectin from orange peel using response surface method and artificial neural network technique. The following findings are absorbed from the effects of extraction parameters and technique used. The pH solution was highly significant compared to ultrasound power. As well as interaction between ultrasound and pH were found to be strongly influenced the extraction yield of pectin. The optimal parameters for extraction were irradiation time of 22.5min, pH of 1.5, and ultrasound power of 155W and liquid-solid ratio 22.5:1 mL/ g. Under these conditions, yield of pectin was 26.87% experimentally, while 26.74 and 26.93% of yield were predicted by response surface and artificial neural network model respectively.The extracted pectin was categorized as high methoxyl pectin, since it has 63.13% degree of esterification, which is above 50% affirmed by Fourier transform infrared spectroscopy detection. Both response surface methodology and artificial neural network model prediction was in good agreement with experimental data; however, the prediction of artificial neural network prediction was better than artificial neural network. Therefore, artificial neural network model is much more accurate in estimating the values of pectin yield and mean square error when compared with the response surface methodology.
Efficacy of a Novel Hemostatic Adhesive Powder in Patients With Upper Gastrointestinal Tumor Bleeding
Authors: Shin Jongbeom , Cha Boram , Park Jin-Seok , Ko Weonjin , Kwon Kye Sook , Lee Jin-Woo , Kim Hyung Kil , Shin Yong Woon , Lee Don Haeng 
Published: October 2020

Abstract
Background: Gastrointestinal tumor bleeding remains a clinical challenge because it is difficult to treat with conventional endoscopic hemostatic options. Recently, an endoscopic hemostatic powder (UI-EWD) was developed and reported to provide effective control of upper gastrointestinal bleeding. The aim of current study was to evaluate the feasibility and efficacy of this novel hemostatic powder in tumor bleeding.Methods: A total of 41 consecutive patients with upper gastrointestinal tumor bleeding were included. UI-EWD was applied in all patients as an auxiliary hemostatic method as a salvage therapy or monotherapy during endoscopic treatment. Hemostasis success rates, adverse event related to UI-EWD, and rates of re-bleeding were evaluated.Results: In all cases, UI-EWD application was successful at tumor bleeding sites. Immediate hemostasis occurred in 40/41 (97.5%) patients, and re-bleeding within 28 days occurred in 10 of 40 (22.5%) patients that achieved initial hemostasis. The success rate of immediate hemostasis for UI-EWD monotherapy was 100% (23/23). The re-bleeding rate at 28 days after UI-EWD monotherapy was 26.1% (6/23). No adverse events associated with UI-EWD application were encountered.Conclusions: The success rate of UI-EWD for immediate hemostasis in cases of GI tumor bleeding was excellent and UI-EWD produced promising results with respect to the prevention of re-bleeding. Based on these results, we suggest that UI-EWD be considered an effective salvage therapy or even monotherapy for GI tumor bleeding.