bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of using a hemostatic gelatin sponge versus a dense polytetrafluorethylene membrane for socket sealing following immediate implant placement. A randomized clinical study
Authors: Shalash Mahmoud , Emara Aalaa 
Published: December 2024

Abstract

Background
Socket sealing following flapless immediate implant placement involves placing a barrier membrane to prevent the ingrowth of bacteria or contaminants to the dental implant during the early phase of healing. Dense polytetrafluoroethylene membranes have been the gold standard socket sealing barrier due to their impermeability to the oral flora, which allows for undisturbed implant healing. The search for a more cost-efficient material, that is easy to handle and apply for sealing the socket without compromising implant osseointegration was sought. This study aims to evaluate the effectiveness of socket sealing after immediate implant placement in the premolar/molar region using a gelatin sponge, as opposed to a dense polytetrafluoroethylene membrane, and its impact on clinical implant osseointegration.
Materials and Methods
The study was conducted on 20 patients, comprising 12 males and 8 females with an age range of 25–60 years. Those meeting the eligibility criteria of immediate implant placement were selected and divided equally into two groups. A control group, where an immediate implant and a dense polytetrafluorethylene membrane was used, and a test group where an immediate implant and a gelatin sponge was used. Exclusion criteria included smokers, presence of systemic or metabolic conditions, or a local pathology that would contraindicate implant placement. Teeth were extracted using a flapless approach. Implants were placed, and sockets were sealed using the allocated barrier. In the control group, the membrane was removed after 4 weeks. For the test group, the gelatin sponge was left to resorb spontaneously. After 4 months, implants were uncovered, and a 30 N/cm reverse torque test was applied to assess the clinical osseointegration.
Results
All implants healed uneventfully, without any radiographic evidence of bone loss. There was no clinical or radiographic difference between the outcomes in the control and the test groups. For the 20 dental implants subjected to a 30 N/cm reverse torque test, clinical integration was observed.
Conclusion
Within the limitations of this study, both dense polytetrafluorethylene membranes and gelatin sponges are effective methods for socket sealing, allowing for successful osseointegration following flapless immediate implant placement in posterior sites, with gelatin sponge offering easier handling and a reduced cost compared to dense polytetrafluorethylene. A larger study sample to verify the outcomes of this study is recommended.
Analysis of Efficacy and Safety of Body Gamma Knife Stereotactic Radiosurgery in Patients with Early- stage Non-small-cell Lung Cancer: A 10-Year Cohort Study
Authors: DENG Xuyan , HU Qilu , LIU Lihua , Kong Dechen , LI Hongqi 
Published: December 2024

Abstract

Introduction: Stereotactic body radiotherapy is frequently used for treating non-operable early-stage non-small cell lung cancer . Body gamma knife stereotactic radiosurgery is a specialized SBRT technique established in China. Here, the long-term efficacy, failure pattern, and toxicity of γ-SRS for initial-phase NSCLC were evaluated through a cohort study, including a 10-year follow-up.
Methods:To conduct this study, 29 patients with initial-phase NSCLC received γ-SRS treatment between 2007 and 2010. The prescribed doses for the Gross Target Volume (GTV), Clinical Target Volume (CTV), and Planning Target Volume (PTV) were 70, 60, and 50 gray (Gy), respectively, administeredin 10 fractions, with the creation of curves for the 50, 60, and 70% isodoses to cover 80, 90, and 100% of the GTV,CTV, and PTV, respectively. Patients underwent CT/PET-CT imaging at one, three, and six months post-treatment, followed by imaging every six months for two years, and later, annual examinations were conducted.
Results:Patients were aged 71 years on average, the mean follow-up was 166 months, overall survival（OS）was 75 months, and the corresponding median progression-free survival (PFS) was 57 months. Moreover, the first, third, fifth, and ten-year OS rates were 93.1, 75.9, 65.5, and 34.9%, while the PFS rates were 86.2, 65.3, 43.6, and 27.7%. Only two patients (6.9%) had grade 3 treatment-associated adverse events（Pneumonia）, while grade 4 or 5 events were not reported.
Conclusion:This study depicted the efficacy of γ-SRS for inoperable early-phase NSCLC patients or those who refused surgery. The primary patterns of failure outcomes were regional relapse and distant metastasis.
A study of the effects of vibration training at different frequencies on lower limb muscle strength in semi-elite short track speed skaters
Authors: Qi Qianrong , Gao Yuanyuan 
Published: December 2024

Abstract

The aim of this study is to examine the impact of lower limb muscle strength in semi-elite athletes engaged in short track speed skating through the lens of different frequency vibration training. The methodology employed in this study is as follows: The study employs an experimental methodology. Subsequent to the screening process, a total of 75 male semi-elite short track speed skaters were selected from the Shenyang Institute of Physical Education. The participants were randomly assigned to one of three groups: a 30 Hz frequency vibration group comprising 25 individuals, a 50 Hz frequency vibration group comprising 25 individuals, and a control group comprising 25 individuals. The experimental group underwent the intervention on two occasions per week, with three groups participating on each occasion. The total duration of the intervention was four weeks, with an amplitude of 2mm. Each session comprised 30 seconds of whole-body vibration training. The control group comprised 25 individuals who performed the same duration and posture of semi-squatting movement on the vibration platform, but without additional vibration stimulation. The maximum strength, rapid strength, and strength endurance of the knee flexors and extensors during centripetal contraction were evaluated both before and after the intervention. The results are presented in the following manner: (1) Maximum power: the peak moments of the knee flexor and extensor muscle groups of both legs of the subjects exhibited a notable enhancement in comparison to the values observed prior to the training period (P < 0.01). The vibration frequency of 50 Hz demonstrated a more pronounced impact on the improvement of the peak moments of the knee flexor muscle groups on both sides. (2) Rapid power: the 50Hz vibration frequency was observed to have a more significant enhancement effect on the peak power of the bilateral knee flexor muscle groups and the left knee extensor muscle groups (P < 0.05). (3) Muscular endurance: a significant difference was observed in the total work of the subjects' bilateral knee flexor and extensor muscle groups with different frequencies of vibration stimulation (P < 0.05), with a highly significant difference noted in the total work of the left knee flexor muscle group (P < 0.01). It can be concluded that four weeks of vibration training at different frequencies can significantly improve the muscle strength of the knee joints in semi-elite short track speed skaters. Furthermore, the results demonstrate that a vibration frequency of 50 Hz is more effective than 30 Hz in terms of maximal and rapid strength when the amplitude is 2 mm.
Scalable Reinforcement Learning for Large-Scale Coordination of Electric Vehicles Using Graph Neural Networks
Authors: Orfanoudakis Stavros , Robu Valentin , Duque Edgar Mauricio Salazar , Palensky Peter , Vergara Pedro 
Published: December 2024

Abstract

As the adoption of electric vehicles (EVs) accelerates, addressing the challenges of large-scale, city-wide optimization becomes critical to ensuring efficient use of charging infrastructure and maintaining electrical grid stability. This study introduces EV-GNN, a novel graph-based solution that addresses scalability challenges and captures uncertainties in EV behavior from a Charging Point Operator's (CPO) perspective. We prove that EV-GNN enhances classic RL algorithms' scalability and sample efficiency by combining an end-to-end Graph Neural Network (GNN) architecture with Reinforcement Learning (RL) and employing a branch pruning technique. We further demonstrate that the proposed architecture's flexibility allows it to be combined with most state-of-the-art deep RL algorithms to solve a wide range of problems, including those with continuous, multi-discrete, and discrete action spaces. Extensive experimental evaluations show that EV-GNN significantly outperforms state-of-the-art RL algorithms in scalability and generalization across diverse EV charging scenarios, delivering notable improvements in both small- and large-scale problems.
Tumor-derived exosomal miR-21-5p mediates lung fibroblasts activation via TNFAIP3/NF-kB signaling to promote non-small cell lung cancer cell migration and invasion
Authors: Fan Youqiang , Hao Jingduo , Cen Xueying , Song Kun , Yang Chunbo , Xiao Shengwen , Cheng Siming 
Published: December 2024

Abstract

MicroRNAs (miRNAs) have been recognized as significant mediators in non-small cell lung cancer (NSCLC). However, their particular effects on NSCLC remain largely unknown. In addition, fibroblasts are essential components of the tumor microenvironment (TME) and play significant roles in tumor development. It has been also reported that exosomes can mediate the communication between tumor cells and fibroblasts in TME. However, the role of extracellular miR-21 in the intercellular communication of tumor cells and fibroblasts remains elusive. The differentially expressed miRNAs between NSCLC and normal tissues were identified by screening the Gene Expression Omnibus (dataset, GSE63805) and The Cancer Genome Atlas databases using R language software. The bioinformatics analysis results showed that compared with normal tissues, miR-21-5p was upregulated in lung cancer tissues, while the increased expression levels of miR-21-5p were associated with worse survival rate in patients with NSCLC. Additionally, exosomes could deliver miR-21-5p from NSCLC cells to lung fibroblasts, thus enhancing the levels of miR-21-5p in fibroblasts. Furthermore, the results demonstrated that tumor necrosis factor α induced protein 3 (TNFAIP3) was a direct target of miR-21-5p in NSCLC cells. These results suggested that exosomal miR-21-5p derived from NSCLC cells could activate lung fibroblasts via targeting TNFAIP3-mediated nuclear factor κB (NF-κB) signaling. Most importantly, exosomal miR-21-5p could facilitate fibroblast activation, which in turn could enhance the migration and epithelial-mesenchymal transition of NSCLC cells. Overall, the findings of the present study revealed that NSCLC cell-derived exosomal miR-21-5p could mediate lung fibroblast activation via the TNFAIP3/NF-kB signaling pathway to promote NSCLC cell migration and invasion. Therefore, targeting exosomal miR-21-5p could provide a potential strategy for the prevention and treatment of NSCLC.
Association between Composite Dietary Antioxidant Index and cataract: a new insight from a cross-sectional study
Authors: Liu Xuefen , Wei Daheng , Hao Haitao , Wang Shuxia , Ding Guanyu , Liu Jiashun , Liu Hua 
Published: December 2024

Abstract

Objectives Growing evidence suggests that elevated CDAI may confer protection against various health conditions, despite the extensive examination of the association between the composite dietary antioxidant index (CDAI), an essential metric of antioxidant intake, and the risk of chronic diseases. However, the correlation between CDAI and the likelihood of age-related cataracts is inadequately characterized. To address this gap, we performed a cross-sectional investigation to evaluate a potential link between CDAI and cataract occurrence. Method The National Health and Nutrition Examination Survey (NHANES), which ran from 2005 to 2008, provided the data for this investigation. We evaluated dietary antioxidants using two 24-hour food recall interviews. To derive CDAI, we concentrated on six specific antioxidants. Based on a history of cataract surgery, we indirectly evaluated age-related cataracts. Logistic regression models were utilized to analyze the correlation between CDAI and cataract risk. Additionally, we investigated nonlinear relationships using curve-fitting methods and performed subgroup analyses to evaluate potential effect modifications. Results This research included 8,582 participants, of whom 5,296 were free of cataracts and 2,881 were diagnosed with cataracts. Compared with normal subjects, the CDAI of cataract patients was lower (-0.32 ± 3.47) (p < 0.001). In multivariate logistic regression, CDAI as a continued variable was inversely correlated with cataract risk [0.95 (00.93–0.97), p < 0.0001]. We looked at CDAI as a categorical variable and discovered that people in Q3 had a much lower risk of cataracts than people in Q1. For each unit increase in CDAI, cataract risk decreased by 15% [0.85(0.72–0.99), p = 0.039]. Subgroup analyses and interaction tests showed that factors like age, sex, level of education, marital status, tobacco use, alcohol consumption, hypertension, hyperlipidemia, and diabetic mellitus did not significantly change the negative relationship between CDAI and cataracts. Conclusions The inverse relationship between the CDAI and cataract risk persisted even after controlling for other possible factors. This suggests that eating a diet rich in antioxidants may reduce the risk of cataract.
Elimination of highly multidrug-resistant wound bacteria by the lactic acid bacterial drug candidate ILP100
Authors: -Tomenius Hava Lofton , Pang Yanhong , Pallin Anton , Myktybekova Zhanar , Lelham Ninus , Riesbeck Kristian , Vågesjö Evelina , Roos Stefan , Phillipson Mia Mia 
Published: December 2024

Abstract

The rise and spread of antibiotic resistance poses a global threat, further exacerbated by geopolitical conflicts. Limosilactobacillus reuteri genetically modified to produce human CXCL12 (ILP100) has previously been shown to accelerate wound healing. Here, we report that ILP100 has potent bactericidal effects against MDR wound pathogens isolated from Ukrainian war victims, suggesting ILP100 as an important therapeutic alternative for eradication of MDR infections, while shortening the time to fully healed.
Giant chlorine isotope effect of ion-molecule reaction
Authors: Tian Shan , Zhi Yaya , Hu Jie , Xie Jingchen , Wu Chun-Xiao 
Published: December 2024

Abstract

Isotope substitution profoundly influences chemical reactions, signifying different reaction rates due to mass-related nuclear quantum effects. Due to the smaller mass-difference ratio, heavy-atom isotope effect is usually much weaker than the hydrogen/deuterium effect, which has been the underlying principle for chemical isotope labelling, a prevailing technique of environmental assessments. Here we report a giant chlorine (35,37Cl) isotope effect of the collisional reactions between iso-dichloroethylene (naturally comprising 35,37Cl-isotopologues) and argon ion. As a grave challenge to conventional understandings, we find that the isotopic signature δ37Cl of the dehydrochlorination channel is one thousand times larger than the regular values, namely, an extraordinary priority of the product C2H37Cl+. Hydrogen-atom scrambling and roaming assisted 35Cl-elimination mechanisms are proposed for this unprecedentedly strong isotope effect. Our finding adds a new dimension of isotope effects and enlightens related methodological renovations of environmental assessment.
WITHDRAWN: Investigation of Factors Affecting Digital Game Addiction in 10-14 Age Group Children
Authors: Güler Funda , Gün Canan Sümeyra , Yıldırım Öznur , Çalışır Hüsniye 
Published: December 2024

Abstract

The full text of this preprint has been withdrawn, as it was submitted in error. Therefore, the authors do not wish this work to be cited as a reference. Questions should be directed to the corresponding author.
Damage evolution mechanism of aluminum alloy stiffened cylinders during flow forming
Authors: Meng Yehui , Yu Zhongqi , Wang Fengqi 
Published: December 2024

Abstract

Fracture is one of typical defects for complex stiffened cylinders during flow forming. The introduction of complex structures makes fracture prediction challenging. This study aims to assess the forming limits of stiffened cylinders during flow forming, alongside the selection of appropriate prediction models. Therefore, a spinnability method was proposed to evaluate the formability of stiffened cylinders and its corresponding finite element model was developed. The results show that cracks initiated at the stiffener downstream fillet in flow forming of AA6061-T6 stiffener cylinders, and then developed along the circumferential direction due to significant axial tensile stresses. Four typical uncoupled damage models were incorporated into the finite element model to simulate the damage evolution in spinnability tests of stiffened cylinders. The prediction results show that all selected models demonstrated an ability to correctly predict the location of cracking, but H-C model offers the most accurate predictions in spinnability tests of AA 6061-T6 stiffened cylinders. These approaches can provide an evaluation method for flow spinning process design of stiffened cylinders.
Fuzzy Mixed Method for Sustainability Assessment of Independent Smallholders’ Oil Palm Plantation Business
Authors: Safriyana Safriyana 
Published: December 2024

Abstract

In terms of productivity and economic benefits, oil palm plantations, particularly those managed by independent smallholders, exhibit rapid development. The urgency of addressing the oil palm plantation sector is highlighted by the disparities in quality and productivity experienced by independent smallholders. Additionally, sustainability issues are crucial due to market concerns about agricultural practices and certification requirements, which demand continuous traceability. The research objectives aimed to model sustainability evaluation using the Indonesian Sustainable Palm Oil (ISPO) standard as the national benchmark. This study designed an adaptive assessment and improvement model specifically for independent smallholders. The methodology included (1) a multidimensional scaling (MDS) analysis to evaluate sustainability performance and (2) an adaptive neuro-fuzzy inference system (ANFIS) to integrate the overall sustainability index. Nineteen indicators of sustainability were categorized into six dimensions: environmental, economic, social, technological, policy, and institutional. The results indicated the following sustainability levels for the independent smallholder plantations: 39.54% for environmental, 43.21% for economic, 42.82% for social, 42.93% for technological, 42.17% for policy, and 41.66% for institutional dimensions. The aggregated sustainability index showed an overall sustainability rate of 42.09%, reflecting a less sustainable condition. Recommendations for improving sustainability are provided based on the specific indicators evaluated.
UBE2T promotes PTC progression by activating the JAK/STAT3 pathway via negative regulation of SOCS2
Authors: Zhang Lijun , Li Chengyuan , Zhou Jianing , Li Lin , Zhang Xiang , Fang Haisheng , Cai Jingsheng , Tong Houchao , Wen Jianfei , Zhang Heda , Shen Meiping , Si Yan 
Published: December 2024

Abstract

Background
Thyroid cancer, particularly papillary thyroid carcinoma (PTC), is one of the most common malignant tumors of the endocrine system. Malignant biological behaviors such as tumor invasion and cervical lymph node metastasis are closely associated with the prognosis of PTC. To date, no effective method has been identified to accurately predict the invasive biological behavior of PTC.
Objective
This study aims to investigate the potential molecular mechanisms underlying the high invasiveness of PTC mediated by UBE2T.
Methods
We examined the expression of UBE2T in PTC using data from the TCGA and GEO databases and validated these findings in clinical samples from our institution, analyzing clinical pathological features. Subsequently, we explored the impact of UBE2T on the biological behavior of PTC cells through stable overexpression or knockdown of the UBE2T gene. Additionally, we elucidated the potential mechanisms by which UBE2T promotes PTC progression, with a particular focus on its role in activating the JAK-STAT signaling pathway.
Results
Our results demonstrate that UBE2T plays a crucial role in promoting PTC progression by activating the JAK-STAT signaling pathway. Correlation analysis and co-immunoprecipitation (co-IP) experiments identified cytokine signaling suppressor 2 (SOCS2) as a key molecule mediating UBE2T's action in the JAK-STAT pathway. Further rescue experiments and immunofluorescence (IF) assays confirmed that UBE2T promotes PTC progression by negatively regulating SOCS2, thereby activating the JAK-STAT3 pathway.
Conclusion
This study reveals the mechanistic role of UBE2T in the high invasiveness of PTC, highlighting its negative regulation of SOCS2 to activate the JAK-STAT3 signaling pathway and drive PTC progression. These findings provide new insights into the mechanisms of PTC invasion and may offer potential therapeutic targets for inhibiting PTC metastasis and recurrence.
Role of AMPK/ACC/SREBP-1 and MAPK Pathways in Glucose Intolerance and Liver Dysfunction of Largemouth Bass
Authors: liao zhihong , he xuanshu , gu xinyu , ye tao , chen anqi , guo yucai , zhao wei , Niu Jin 
Published: December 2024

Abstract

Background and Objectives Largemouth bass (Micropterus salmoides), a carnivorous fish, struggles to process dietary carbohydrates, often resulting in energy metabolism issues and fatty liver disease. This study explored the liver glycolipid accumulation and mitochondrial dysfunction in both largemouth bass and primary hepatocytes treated with high glucose.Methods An 8-week feeding experiment was conducted to evaluated the effects of high carbohydrate (HC) diet on liver morphology, fatty acid metabolism, bile acid metabolism, and MAPK signaling pathways in largemouth bass. Primary hepatocytes were treated with high-glucose (HG) medium to simulate the conditions of high carbohydrate feeding to further evaluated the effects of high-glucose treatment on cell growth, ROS production, antioxidant capacity, mitochondrial fusion, fission, mitophagy and mitochondrial apoptosis.Results The results showed that the HC diet significantly increased the hepatosomatic and visceral somatic indices, causing liver enlargement, mitochondrial damage, and glycolipid buildup. Compared to a control diet, the HC diet enhanced lipid synthesis through the AMPK/ACC/SREBP-1 pathway and increased the phosphorylation levels of ERK, JNK and p38 MAPK, while it decreased bile acid synthesis by downregulating cholesterol 7-hydroxylase (CYP7A1) and sterol 12-hydroxylase (CYP8B1). In vitro experiments showed that high glucose (HG) treatment in primary hepatocytes inhibited cell growth, promoted apoptosis, increased reactive oxygen species (ROS), and reduced antioxidant capacity. Mechanistically, HG treatment led to mitochondrial fission and damage. Damaged mitochondria bind to autophagosomes for lysosomal degradation, resulting in mitochondrion-dependent apoptosis by regulating p38 MAPK/BCL-2/CAS3 signaling pathway.Conclusions High glucose could not only induce accumulation of lipid and glycogen mediated by the AMPK/ACC/SREBP-1 signaling pathway, but could activate p38MAPK-mediated signaling to induce mitochondrial apoptosis.