bluuebunny/crossref_metadata_2025_split huggingface dataset:
ASPN is a Potential Promising Biomarker for Common Heart Failure
Authors: Zhang Kai , Wu Min , Qin Xianyu , Zhou Xianwu , Zhou Jianrong , Chen Shaoxian , Wen Pengju , Wu Yueheng , Zhuang Jian 
Published: June 2020

Abstract
Background: Heart failure (HF), the leading cause of adult mortality and morbidity worldwide, is the end-stage of various diseases, especially ischemic cardiomyopathy (ICM) and dilated cardiomyopathy (DCM). This study aimed to investigate the common molecular mechanism of ICM and DCM.Methods: Four gene expression datasets, GSE1869, GSE5406, GSE57338, and GSE79962, were obtained from the Gene Expression Omnibus (GEO) database. The differentially expressed genes (DEGs) of ICM or DCM samples compared with those of nonfailing samples were identified. Gene ontology (GO) annotation, Kyoto encyclopedia of gene and genome (KEGG) pathway analysis, and the protein-protein network (PPI) of the coregulated DEGs in at least three datasets were performed using the online tools of DAVID, the KOBAS database, and the STRING database, respectively. Hub genes of HF were analyzed for their correlation with left ventricular ejection fraction (LVEF) in dataset GSE19303. The expression levels of notable DEGs were further validated in our tissue microarray (TMA).Results: Fifty-nine coregulated ICM and sixty-eight coregulated DCM relevant DEGs were identified (in at least three datasets). Moreover, 38 common DEGs between ICM and DCM relevant DEGs were obtained that were mainly involved in inflammatory/stress processes, proliferation, and some lipid metabolism pathways. Among the ten hub genes with top degrees, four genes showed a correlation with LVEF, and ASPN had the most significant correlation. Finally, the expression of ASPN protein was validated in our TMA and was significantly increased in ICM and DCM left ventricular samples.Conclusion: The present study revealed some common molecular mechanisms of HF with different causes. Furthermore, ASPN may be a potential promising biomarker for HF.
Compared with Two-Stage Revision, Destination Joint Spacers Have a Similar Infection-Relief Rate and a Higher Complication Rate
Authors: Cai Yuanqing , Fang Xinyu , Lin Yi , Huang Zida , Zhang Chaofan , Li Wenbo , Zhang Wenming , Guan Zhenpeng 
Published: June 2020

Abstract
Background: Two-stage revision is regarded as the “gold standard” for periprosthetic joint infection (PJI) treatment, in which antibiotic-impregnated spacers play an important role. However, some patients are unable to undergo reimplantation of the prosthesis after spacer implantation. The clinical outcomes of these patients remain unknown. The purpose of this research was to study the infection-relief rate and clinical outcome of these patients.Methods: From January 2006 to December 2017, data from PJI patients who underwent implantation with antibiotic-impregnated cement spacers in our center due to chronic PJI were collected retrospectively. Age, sex, body mass index (BMI) and laboratory test results were recorded, and the infection-relief rate and clinical outcomes were observed.Results: A total of 62 patients who were diagnosed with PJI were enrolled, with an age of 65.13 ±9.94 (39-88) years. There were 21 cases in the destination joint spacer group and 41 cases in the temporary spacer group (reimplantation of prosthesis after infection relief). The Charlson comorbidity index (CCI) in the destination joint spacer group was higher than that in the temporary spacer group. Our study showed that the infection-relief rate of destination joint spacers was similar to that of two-stage revision, but the incidence of complications was higher than that of two-stage revision, especially for the type I spacer used in this study.Conclusions: The infection-relief rate of destination joint spacers was similar to that of two-stage revision, but the complication rate was higher than that of two-stage revision (especially for the type I spacer).
Clinical Characteristics of Epstein–Barrvirus Infection in the Pediatric Nervous System
Authors: Cheng Huan , Chen DouDou , Peng XiaoLing , wu Peng , Jang Li , Hu Yue 
Published: June 2020

Abstract
Background: To investigate the clinical characteristics of Epstein–Barr virus (EBV) infection in the pediatric nervous system (NS).Methods: We retrospectively analyzed the clinical data and follow-up results of 89 children with neurological damage caused by EBV who were hospitalized in the children's hospital of Chongqing Medical University from January 2008 to April 2019.Results: EBV infection of the NS can occur at any time of the year. The highest incidence was seen in the age group of 0–4 years. Fever is the main clinical feature (74/89, 83.1%). The main clinical types were encephalitis/meningoencephalitis (64/89, 71.9%), acute myelitis (2/89, 2.2%), acute disseminated encephalomyelitis (ADEM) (3/89, 3.4%), Guillain–Barré Syndrome (GBS) (15/89, 16.9%), neurological damage caused by EBV-hemophagocytic lymphohistiocytosis (EBV-HLH) (4/89, 4.5%), and NS-post-transplant lymphoproliferative disorder (NS-PTLD) (1/89, 1.1%). Anti-N-methyl-D-aspartate receptor encephalitis was found during the convalescence of EBV encephalitis. EBV encephalitis/meningitis showed no symptoms of tonsillitis, lymph node enlargement, skin rash, hepatosplenomegaly. Acute motor axonal neuropathy is the chief complication in GBS caused by EBV.Conclusion: There were significant differences in neurological complications caused by EBV. The prognosis of EBV infection in the NS is generally good. These illnesses are often self-limiting. A few cases may show residual sequelae.
Serum Neutrophil Gelatinase-Associated Lipocalin at 3 Hours After Return of Spontaneous Circulation in Patients with Cardiac Arrest and Therapeutic Hypothermia: Early Predictor of Acute Kidney Injury
Authors: Choi Yoon Hee , Lee Dong Hoon , Lee Jae Hee 
Published: June 2020

Abstract
Background: Neutrophil gelatinase-associated lipocalin (NGAL) could be used as a predictive marker of acute kidney injury (AKI) in patients with return of spontaneous circulation (ROSC) after out-of-hospital cardiac arrest (OHCA) who are managed with targeted temperature management (TTM). However, the NGAL measurement timepoints vary. The primary objective of this study was to determine an association between AKI and NGAL, both immediately (ROSC-NGAL) and 3 hours after ROSC (3h-NGAL), in OHCA patients with TTM. The secondary objective was to ascertain the association between NGAL levels in the early post-ROSC phase and the neurologic outcomes at discharge.Methods: This prospective observational study was conducted between January 2016 and December 2018 and enrolled adult OHCA patients (≥18 years) with TTM after ROSC. Serum NGAL was measured immediately and 3 hours after ROSC. Univariate and multivariate analysis was performed to identify the associations between AKI, poor neurologic outcome, and NGAL.Results: Among 861 OHCA patients, 89 patients were enrolled. AKI occurred in 48 (55.1%) patients. On multivariate logistic regression analysis, 3h-NGAL was significantly associated with AKI (odds ratio [OR] 1.022; 95% confidence interval [CI] 1.009–1.035; p = 0.001). The AUROC of 3h-NGAL for AKI was 0.910 (95% CI 0.830–0.960), and a cut-off value of 178 ng/mL was identified. Both ROSC-NGAL and 3h-NGAL were not significantly associated with poor neurologic outcome on multivariate logistic regression analysis (ROSC-NGAL; OR 1.017; 95% CI 0.998–1.036; p = 0.084, 3h-NGAL; OR 0.997; 95% CI 0.992–1.001; p = 0.113) Conclusions: The serum NGAL concentration measured 3 hours after ROSC is an excellent early predictive marker for AKI in OHCA patients treated with TTM. Future research is needed to identify the optimal measurement timepoint to establish NGAL as a predictor of neurologic outcome and to validate the findings of this research.
Caspase 4 Overexpression in Clear Cell Renal Cell Carcinoma and Its Prognostic Role
Authors: Meng Lingfeng , Tian Zijian , Long Xingbo , Diao Tongxiang , Hu Maolin , Wang Miao , Zhang Wei , Zhang Yaoguang , Wang Jianye , He Yuhui 
Published: June 2020

Abstract
Background: Caspase 4 (CASP4) dysregulation is related to the occurrence, development, and outcome of many malignant tumors, but its role in clear cell renal cell carcinoma (ccRCC) is unclear. This study was conducted to investigate the expression level of CASP4 in tumor tissues and its relationship with clinical prognosis of patients with ccRCC. Methods: First, the Oncomine and The Cancer Genome Atlas databases were used to determine CASP4 mRNA expression in ccRCC and its association with ccRCC prognosis. We then performed immunohistochemical staining and evaluation of 30 paired ccRCC and adjacent normal tissues to confirm these results. The correlation between CASP4 expression and ccRCC prognosis was evaluated using Kaplan-Meier analysis, and related genes and pathways were obtained from The Cancer Genome Atlas database by gene set enrichment analysis and gene set variation analysis. Finally, we explored the co-expression of genes with CASP4 in ccRCC. Results: CASP4 mRNA expression in ccRCC was significantly higher than that in normal tissues (p < 0.001). Kaplan-Meier analysis showed that the overall survival of patients with ccRCC showing high CASP4 expression was significantly reduced (p < 0.001). We then used external datasets (Gene Expression Omnibus database and patients from our center) to verify the level of CASP4 expression and survival differences (all p < 0.05). We also found that differential expression levels of CASP4 were correlated with pathological grade and clinical TNM stage (all p < 0.05). Conclusions: Overall, our study shows that CASP4 is highly expressed in ccRCC and is an important factor affecting prognosis. Thus, CASP4 may be a potential prognostic biomarker of ccRCC.
LncRNA LINC00342 contributes to the growth and metastasis of colorectal cancer via targeting miR-19a-3p/NPEPL1
Authors: Shen Peng , Qu Lili , Wang Jingjing , Ding Quchen , Zhou Chuanwen , Xie Rui , Wang Honggang , Ji Guozhong 
Published: June 2020

Abstract
Background Long intergenic non-protein coding RNA 342 (LINC00342) has been identified as a novel oncogene, however, the functional role of LINC00342 in colorectal cancer (CRC) remained unclear. Methods The expression of LINC00342 was detected by real-time PCR. Cell proliferation, migration and invasion and xenograft model were examined to analyze the biological functions of LINC00342 in vitro and in vivo. Dual-luciferase reporter and RNA immunoprecipitation (RIP) assays were used to identify the target interactions between LINC00342, miR-19a-3p and aminopeptidase like 1 (NPEPL1). Results LINC00342 was highly expressed in CRC. Downregulation of LINC00342 inhibited cell proliferation and metastasis of CRC cells. Moreover, knocking down LINC00342 could weaken the tumor growth in vivo. Mechanistic investigation revealed that LINC00342 may sponge miR-19a-3p to regulate NPEPL1 expression. Further investigation indicated that the oncogenesis facilitated by LINC00342 was inhibited by NPEPL1 depletion.Conclusion LINC00342 promoted CRC progression by competitively binding miR-19a-3p with NPEPL1.
Knockdown of lncRNA LINC01234 Suppresses The Tumorigenesis of Liver Cancer via Sponging miR-513a-5p
Authors: Xu Wen , Li Kesang , Song Changfeng , Wang Xiaotong , Li Yueqi , Bai Xue , Liang Xin , Deng Wanli , Wang Junqing , Liu Jianwen 
Published: June 2020

Abstract
Background: Liver cancer is a frequent malignancy with poor prognosis. It has been reported that many lncRNAs could regulate the progression of liver cancer. To identify potential therapeutic targets for liver cancer, we conducted bioinformatics analysis of lncRNAs in tumor tissues and adjacent normal tissues. Methods: The differential expression of lncRNAs between liver cancer tissues and adjacent normal tissues were examined by bioinformatics analysis. Cell proliferation was tested by CCK-8. Cell apoptosis in liver cancer was detected by flow cytometry. Gene and protein expression in liver cancer cells were measured by q-PCR and Western-blot, respectively. Xenograft tumor model was established to verify the function of LINC01234 on liver cancer in vivo.Results: LINC01234 was found to be notably upregulated in liver cancer tissues. In addition, knockdown of LINC01234 significantly inhibited the proliferation, invasion and induced the apoptosis of liver cancer cells. Meanwhile, miR-513a-5p was a downstream target of LINC01234 and USP4 was a direct target of miR-513a-5p. Moreover, downregulation of LINC01234 inhibited the tumorigenesis of liver cancer via inactivating TGF-β signaling.Conclusion: Downregulation of LINC01234 could inhibit the progression of liver cancer. Thus, LINC01234 may serve as a potential novel target for treatment of liver cancer.
Rapid screening for individualized chemotherapy optimization of colorectal cancer: a novel conditional reprogramming technology-based functional diagnostic assay.
Authors: Li YingJie , Guo Dagang , Zhang Yihong , Wang Lin , Sun Tingting , Li Zhongwu , Zhang Xiaoyan , Wang Shuai , Chen Yiyou , Wu Aiwen 
Published: June 2020

Abstract
In vitro patient tumor models such as patient-derived organoids (PDO) and conditionally reprogrammed (CR) cell culture are important for translational research and pre-clinical drug testing.In this study we present a personalized drug sensitivity test for late stage, potentially operable colorectal cancer (CRC) using patient-derived primary cell culture based on a new generation CR technology. We explored the clinical feasibility of using CR-based primary cell culture system to guide CRC chemotherapy, and established the correlation between in vitro drug sensitivity and patient clinical response.Our novel CR platform (termed i-CR) can be used to propagate primary colorectal tumor cells that represent individual patient tumors effectively by keeping the clonal heterogeneity and comparable drug responses.Therefore, our platform can be used to test and optimize therapeutic regimens pre-clinically, study cancer cell biology, and model tumor re-emergence to identify new targeted therapeutics from an effective personalized medicine standpoint.
Disruption of Plasmodium Falciparum Histidine-Rich Protein II can affect Heme Metabolism in the Blood Stage
Authors: Yang Yingchao , Tang Tongke , Feng Bo , Li Shanshan , Hou Nan , Ma Xiao , Jiang Lubin , Xin Xiaofang , Chen Qijun 
Published: June 2020

Abstract
Background. Heme is a key metabolic factor in the life of malaria parasite. In the blood stage it acquires host hemoglobin to generate amino acids for its own protein synthesis and by-product heme for metabolic use. The malaria parasite also can de novo synthesize heme by itself. Plasmodium falciparum-specific histidine-rich protein 2 (PfHRP2) is a histidine-rich protein and has a heme-binding site to mediate hemozoin formation, a bio-crystallized form of heme-aggregates. It is interesting to investigate the vibration of hemoglobin-derived heme metabolism and de novo heme-biosynthetic pathway in the Pfhrp2 disrupted parasites during the intraerythrocytic stages. Methods. A CRISPR-Cas9 system was used to disrupt the gene locus of Pfhrp2. DNA was extracted from the transgenic parasites and polymerase chain reaction (PCR), western blotting and southern blotting were used to manifest the successful establishment of transgenic parasites. RNA-seq and comparative transcriptome analysis were performed to identify the difference in gene expression between 3D7 and Pfhrp2- 3D7 parasites. Results. Pfhrp2- transgenic parasites were successfully established by the CRISPR/Cas9 system. The disruption of the exon 2 of Pfhrp2 can down-regulate the gene expression of Pfhrp3 which involved in hemoglobin-derived heme metabolism. It also up-regulates the gene expression level of enzymes of heme biosynthesis.Conclusion: These data support that although Pfhrp2 is a dispensable gene for intraerythrocytic stages parasite but heme metabolism’s stabilizing is very important. The disruption of Pfhrp2 can both affect the pathway of heme metabolism and biosynthesis. A co-operation mechanism may exist between the heme biosynthesis and metabolism pathways for parasite growth in blood stage.
Clients and professionals elicit long-term care preferences by using ‘What matters to me’: A process evaluation
Authors: Leersum Catharina Margaretha van , Moser Albine , Steenkiste Ben van , Wolf Judith R.L.M. , Weijden Trudy van der 
Published: June 2020

Abstract
Background
‘What matters to me’ is a five-category preference elicitation tool to assist clients and professionals in choosing long-term care. This study aimed to evaluate the use of and experiences with this tool.
Methods
A mixed-method process evaluation was applied. Participants were clients, relatives, and professionals. They were all involved in decision-making on long-term care. Data collection comprised online user activity logs (N = 71), questionnaires (N = 38), and interviews (N = 20). Descriptive statistics was used for quantitative data, and a thematic analysis for qualitative data.
Results
Sixty-nine percent of participants completed one or more categories in an average time of 6.9 (± 0.03) minutes. The tool was rated 6.63 (± 0.88) out of seven in the Post-Study System Usability Questionnaire (PSSUQ). Ninety-five percent experienced the tool as useful in practice. Suggestions for improvement included a separate version for relatives and a non-digital version. Although professionals thought the potentially extended consultation time could be problematic, all participants would recommend the tool to others.
Conclusion
‘What matters to me’ seems useful to assist clients and professionals with preference elicitation in long-term care. Evaluation of the impact on consultations between clients and professionals by using ‘What matters to me’ is needed.
Identification of a Repressor for the Two iol Operons Required for Inositol Catabolism in Geobacillus Kaustophilus
Authors: Yoshida Ken-ichi , Shirae Yusuke , Nishimura Ryo , Fukui Kaho , Ishikawa Shu 
Published: June 2020

Abstract
BackgroundGeobacillus kaustophilus HTA426, a thermophilic Gram-positive bacterium, grows on inositol as its sole carbon source, and an iol gene cluster required for inositol catabolism has been postulated with reference to the iol genes in Bacillus subtilis. The iol gene cluster consists of two tandem operons induced in the presence of inositol; however, the mechanism underlying the induction remains unclear. B. subtilis iolQ is known to be involved in the regulation of iolX encoding a scyllo-inositol dehydrogenase, and its homolog in HTA426 was found two genes upstream of the first gene (gk1899) of the iol gene cluster and termed as iolQ in G. kaustophilus.ResultsWhen iolQ was inactivated, not only the myo-inositol dehydrogenase activity in the cell due to the expression of gk1899 but also the transcription of the two iol operons became constitutive. IolQ was produced and purified as a C-terminal His-tag fusion in Escherichia coli and subjected to the in vitro gel mobility shift assay to examine its DNA binding property. It was observed that IolQ bound to the DNA fragments containing each of the two iol promoter regions, and its DNA binding was antagonized by myo-inositol. Moreover, DNase I footprint analyses were conducted to determine the two binding sites of IolQ within each of the iol promoter regions. By comparing the sequences of the binding sites, a consensus sequence for IolQ binding was deduced to be a palindrome of 5′-RGWAAGCGCTTSCY-3′ (where R = A or G, W = A or T, S = G or C, and Y = C or T).ConclusionIolQ functions as a transcriptional repressor regulating the induction of the two iol operons responding to myo-inositol.
Evaluation of maize lodging resistance based on the critical wind speed of stalk breaking during the late growth stage
Authors: Xue Jun , Ming Bo , Xie Ruizhi , Wang Keru , Hou Peng , Li Shaokun 
Published: June 2020

Abstract
Background
The accurate evaluation of the stalk-lodging resistance during the late stage of maize growth can provide a basis for the selection of cultivars, the evaluation of cultivation techniques, and timely mechanical grain harvesting. In this study, the critical wind speed of stalk breaking, plant morphology, stalk mechanical strength, and lodging rate were investigated in 10 maize cultivars to identify the parameters as evaluate lodging resistance during the later growth stage of maize, and clarify the relationship with the stalk mechanical strength, critical wind speed of stalk breaking, and natural lodging rate in the field.
Results
The results showed that, in the late growth stage, with increasing number of days after physiological maturity, (1) the stalk lodging rate gradually increased, (2) the stalk breaking force and rind penetration strength (RPS) of the third internode above the soil gradually decreased, and (3) the critical wind speed of stalk breaking increased first and then decreased, and was highest at about 16–24 days after physiological maturity. Furthermore, the stalk lodging rate was significantly negatively correlated with the critical wind speed of stalk breaking, however was not correlated with plant height, ear height, stalk breaking force, or the RPS. Additionally, the critical wind speed of stalk breaking was significantly positively correlated with the stalk breaking force and the RPS.
Conclusion
This indicates that the critical wind speed of stalk breaking is a superior way to determine the stalk lodging resistance compared to traditional indicators. These results suggest that, in the late growth stage, the decrease in the stalk mechanical strength is an important reason for the decrease in the critical wind speed of stalk breaking and the increase in the lodging rate.
Micro-haplotyping in polyploids using massively parallel amplicon sequencing
Authors: Geest Geert van , Willemsen Johan , Gujjula Krishna Reddy , Siddavatam Prasad , Willis Christopher Roy , Ramadhar Ravindra , Voorrips Roeland E , Arens Paul 
Published: June 2020

Abstract
Background

Haplotyping in polyploids is a very relevant but challenging task. Most methods based on short-read technologies require haplotype assembly, which is error-prone and computationally intensive. However, most (auto)polyploids have high SNP densities. Therefore, haplotyping could already be done in regions of a size that can be entirely covered by reads generated by current short-read sequencing techniques. Here, we used a method that enables massively parallel amplicon sequencing in order to generate such micro-haplotypes in autotetraploid potato and autohexaploid chrysanthemum.
Results

For potato, we generated 79 million reads to sequence 412 regions in 96 samples. The average region size was 154 base-pairs. On average, we found 2.4 haplotypes per locus per individual. For chrysanthemum, we generated a similar amount of reads to sequence 940 regions in 92 samples. The average region size here was 171 base-pairs. On average, 2.7 haplotypes per locus per individual were found. Concordance with dosage data based on SNP-arrays was up to 96.8% in potato and 94.2% in chrysanthemum.
Conclusions

Interpretation of genotyping data generated with next-generation sequencing can be challenging in polyploids, because estimation of allele dosages is often a requisite. Taking advantage of high SNP densities in many autopolyploids we show that massively parallel amplicon sequencing can generate high-quality data with high information content. This has a broad range of applications, including linkage mapping, diversity studies, and genomic selection.