bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of BRCA1 and BRCA2 mutations on endometrial carcinoma survival rates
Authors: Wang Aifen , Holloway Robert W , Zhao Zihan , Zhang Ziyue 
Published: April 2020

Abstract
Background: To evaluate Effect of BRCA1 and BRCA2 mutations on endometrial carcinoma survival rates. Methods : Data were collected from The Cancer Genome Atlas endometrial cancer database for pathogenic mutations in BRCA1 (58) and BRCA2 (77), coexisting BRCA1 /2 mutations (40), and non-mutations (461). Clinicopathological features and survival rates were compared. Survival time was analyzed using combined data and Cox proportional hazard models, with BRCA1 and BRCA2 as time-varying covariates. Results: Patients with BRCA1 mutations presented with higher risk disease (endometrioid endometrial carcinoma grade 3 and uterine serous carcinoma) than non-carriers and BRCA2 mutation carriers ( p = 0.005 non carriers vs BRCA1 mutation carriers, p = 0.008 BRCA1 mutation carriers vs BRCA2 mutation carriers). BRCA1 and BRCA2 mutation carriers tended to have more endometrioid endometrial carcinoma grade 3 than non-carrier group. Overall survival (OS) rates were higher for all patients with BRCA1 and BRCA2 mutations than non-carriers. Patients with BRCA2 mutations had the most favorable progression-free survival (PFS), followed by patients with BRCA1 and BRCA2 co-mutations, and then BRCA1 alteration carriers ( p = 0.011). BRCA1/2 non-carriers had the worst PFS and OS. Conclusions: Patients with BRCA1 mutations presented with higher risk disease than non-carriers and BRCA2 mutation carriers. BRCA1 and BRCA2 mutation carriers had more favorable OS and PFS than non- BRCA mutation carriers in patients with endometrioid endometrial carcinoma and uterine serous carcinoma.
Study on the Mechanism of Buyang Huanwu Decoction for the treatment of Ischemic Stroke Based on Network Pharmacology
Authors: Wang Kai , Lei Lu , Cao Jinyi , Qiao Yi , Liang Ruimin , Duan Jialin , Feng Zhijun , Ding Yi , Ma Yang , Zhang Enhu , Yang Zhifu 
Published: April 2020

Abstract
Background: Buyang Huanwu Decoction (BYHWD) is one of the representative prescriptions for tonifying qi and promoting blood circulation. This formula has been widely used in Chinese clinical practice for treatment and prevention of ischemic cerebral vascular diseases. However, the mechanism and active compounds of BYHWD used in clinical practice for ischemic stroke are not well understood. The purpose of this study was to understand the potential active components of BYHWD and further explore its mechanism of improving ischemic stroke. Methods: This study was based on network pharmacology and bioinformatics analysis. The compounds of BYHWD were obtained from public databases. Oral bioavailability as well as drug-likeness were screened by using absorption, distribution, metabolism, and excretion (ADME) criteria. Then, components of BYHWD, candidate targets of each component and known therapeutic targets of ischemic cerebral were collected. A network of compound-target genes and compound-ischemic cerebral was established by means of network pharmacology data sources. The enrichment of key targets and pathways was analyzed by using string database and DAVID database. In addition, we verified three key targets predicted by western blot analysis (IL6, VEGFA and HIF1A). Results: Network pharmacology analysis results of BYHWD identified 7 herbs, 42 compounds and 79 target genes associated to cerebral ischemia. The 10 key compounds were baicalein, beta-carotene, Baicalin, kaempferol, luteolin, quercetin, hydroxysafflor yellow A, isorhamnetin, Bifendate, formononetin,Calycosin, AstragalosideIV, Stigmasterol, sitosterol, Z-ligustilide, Dihydrocapsaicin. Core genes in this network were IL6, TNF, VEGFA, HIF1A, MAPK1, MAPK3, JUN, STAT3, IL1B and IL10. And pathways TNF, IL-17, Apoptosis, PI3K-Akt, Toll-like receptor, MAPK, NF-kappa B and HIF-1 signaling pathway, etc. related to ischemic stroke were identified. In vitro experiments, The results showed that compared with the control group (no treatment), BYHWD could significantly inhibit the expression of IL6 and increased the expression of HIF1A and VEGFA. Conclusions: Network pharmacology analysis can reveal close interactions between multi-components and multi-targets, and enhance our understanding of the potential effects of BYHWD in cerebral ischemia.
Prognostic Nomogram Incorporating Immunohistochemical Results for the Overall Survival of Patients with Bladder Cancer.
Authors: Wang Tianwei , Wang Yunyan 
Published: April 2020

Abstract
Objectives: In this study, we want to combine GATA3, VEGF, EGFR and Ki67 with clinical information to develop and validate a prognostic nomogram for bladder cancer.Methods: A total of 188 patients with clinical information and immunohistochemistry were enrolled in this study, from 1996 to 2018. Univariable and multivariable cox regression analysis was applied to identify risk factors for nomogram of overall survival (OS). The calibration of the nomogram was performed and the Area Under Curve (AUC) was calculated to assess the performance of the nomogram. Internal validation was performed with the validation cohort., the calibration curve and the AUC were calculated simultaneously.Results: Univariable and multivariable analysis showed that age (HR: 2.229; 95% CI: 1.162-4.274; P=0.016), histology (HR: 0.320; 95% CI: 0.136-0.751; P=0.009), GATA3 (HR: 0.348; 95% CI: 0.171-0.709; P=0.004), VEGF (HR: 2.295; 95% CI: 1.225-4.301; P=0.010) and grade (HR: 4.938; 95% CI: 1.339-18.207; P=0.016) remained as independent risk factors for OS. The age, histology, grade, GATA3 and VEGF were included to build the nomogram. The accuracy of the risk model was further verified with the C-index. The C-index were 0.65 (95% CI, 0.58-0.72) and 0.58 (95% CI, 0.46-0.70) in the training and validation cohort respectively. Conclusions: A combination of clinical variables with immunohistochemical results based nomogram would predict the overall survival of patients with bladder cancer.
A Qualitative Study of User Perspectives on Outcomes of Orthotic Interventions
Authors: Hall Natalie , Parker Daniel , Williams Anita Ellen 
Published: April 2020

Abstract
Background: Orthotic services offer various health benefits and it is estimated that for every £1 spent on orthotic care the savings to the NHS are up to £4. Several reports into orthotic services in the UK have reported a lack of data relating to outcomes of orthotic care. This lack of data means it is difficult to demonstrate the overall efficacy of orthotic services. There is also lack of clarity about the influences on positive outcomes and we do not know what outcomes are relevant to users. This qualitative study aimed to explore the influences on outcomes of orthotic interventions and opinions on outcome measurement from the user’s perspective. Method: An advisory group involving experts in the field along with a review of the literature was conducted. From this, semi-structured questions were developed, ethical approval obtained, and a focus group was undertaken with five users. Data was transcribed and analysed using thematic analysis.Results: The participants expressed a need for orthotic provision which enables them to function physically and psychologically in the context of all life’s activities. Current practice neglects the impact of altered body image and there is dissatisfaction related to waiting times, impacting on their desired outcomes. However, they expressed positive thoughts about the patient professional relationship and being involved in decision making. They identified fundamental issues around usability of three current outcome measure tools with no one outcome measurement tool being suitable for their complex conditions and interventions. Conclusions: Communication of the clinicians understanding about the physical, psychological and socio-economic impact of living with a chronic condition, being involved in decision making, continuity of care and timeliness of provision were identified as key factors influencing positive health outcomes. Due to the complexity of conditions presenting to orthotic services it would seem that there needs to be a range of outcome measures specific to the user’s condition and the orthoses provided for it. Mutually agreed and individualised goal setting may be a solution that ensure the user can self-monitor positive health gains and services can provide evidence for effective outcomes.
Does massive bowel resection in newborns affect further immunity in children ?
Authors: Sznurkowska Katarzyna , Borkowska Anna , Jankowska Agnieszka , Malanowska Magdalena , Zagierski Maciej , Zieliński Maciej , Trzonkowski Piotr , Czauderna Piotr , Łosin Marcin , Sidorkiewicz Agnieszka Szlagatys 
Published: April 2020

Abstract
Background

Short bowel syndrome (SBS) is defined as the a malabsorptive condition most often caused by massive resection of the small intestine. In children most cases of SBS originate in the newborn period and result from congenital anomalies or necrotizing enterocolitis. Loss of gut mucosa during resection does not only mean loss of absorption surface, but also deprives organism of many immunocompetent cells concentrated in gut associated lymphoid tissue, which is regarded the largest immune organ in humans.
Aim of the study:

We have aimed to access the influence of bowel resection on adaptive immunity in children, basing on peripheral lymphocyte populations and serum immunoglobulins.
Patients and methods:

18 children, who underwent bowel resection in the first month of life and required further home parenteral nutrition were enrolled into the study. 12 healthy children, constituted control group. Based on flow cytometry the following subpopulations of lymphocytes were evaluated: T, B, NK, CD4+, C8 + and activated T cells. Serum immunoglobulins were determined with the use of immunoturbidimetric method.
Results

The percentage of B lymphocytes was reduced, while the rates of lymphocytes T and CD8 + lymphocytes were higher compared to healthy children. We documented significantly lower absolute count and proportion of NK cells in SBS group than in the control group. Absolute counts of lymphocytes, lymphocytes B, T, CD4 + and percentages of lymphocytes CD4+, and activated T cells inversely correlated with the time after resection. No statistically significant differences were found between the levels of IgA, IgM and IgG in the studied and the control group
Conclusions

Children with SBS do not present with clinical signs of immunodeficiency as well as deficits in peripheral lymphocyte populations and serum immunoglobulins. Lower number of NK cells in SBS patients compared to healthy children needs to be verified in larger cohort. The tendency of the lymphocyte subpopulations to decrease over time after resection points out the necessity for longer follow- up.
Retrospective analysis of the clinical features of outpatients with novel coronavirus in areas outside Wuhan, China
Authors: Li Yong-Tao , Peng Mei-Lian , Fu Jia-dan , Liu Li , Guo Yong-zheng , Huang Jian-Rong , Sheng Ji-Fang , Li Lan-Juan , Xu Kai-jin 
Published: April 2020

Abstract
Objective: The novel coronavirus pneumonia (COVID-19) has spread rapidly across the globe with the movement of people. How to diagnose COVID-19 quickly and accurately is a concern for all. We retrospectively assessed the clinical characteristics of patients with COVID-19 detected by outpatient screening in areas outside Wuhan, China, to guide early screening outside the epidemic area, to isolate and treat COVID-19-positive patients, and to control the spread of this virus in the region.Results: Among the 213 patients treated in the fever clinic of our hospital, 41 tested positive for novel coronavirus (2019-nCoV) and 172 were negative. Among the positive patients, 13 (31.7%) of the patients had been to Wuhan, while 28 (68.3%) had not been to Wuhan. There were 4 cases of clustering occurrence. The main symptoms exhibited by COVID-19-positive patients were fever (87.8%), cough (68.3%), and expectoration (34.1%). The C-reactive protein (CRP) levels were increased in 35 (85.3%) positive patients; the hydroxybutyrate dehydrogenase in the myocardial zymogram was increased in 22 positive patients (53.6%) and 38 negative patients (22.1%); computed tomography (CT) findings revealed lung lesions in all 41 positive patients (100%).Conclusion: We classified the patient population and analyzed the data to understand the early clinical performance of COVID-19. Our research illustrate that screening for COVID-19 outside Wuhan should focus on early symptoms such as fever and cough, in combination with lung CT findings, epidemiological history, and sputum pathogen detection to determine whether patients need further isolation.
Etika Kepemimpinan Kristen dalam Perspektif Poskolonial: Studi Kasus Program Pelatihan Kepemimpinan dan Manajemen Mahasiswa di Universitas Kristen Duta Wacana
Authors: Mintardjo Jeannette Josephine , Wijaya Yahya 
Published: April 2020

AbstractThis article explores leadership paradigms employed in the Management and Leadership Training Program for Students (P2KMM) in Universitas Kristen Duta Wacana. An evaluation of the leadership paradigm is important because it plays a determining role in whether a leader would be successful or failed ethically. Using post-colonial approach, this study focuses on the moral spirit acknowledged as the Duta Wacana Values, particularly the value of ‘Service to the World.’ As a part of the research, a fieldwork was carried out both by observation of 2017 P2KMM writing materials, as well as interviews with representatives of P2KMM committee and the student staff . The finding indicates that the said value tends to be overlooked in the UKDW leadership program for students.
AbstrakArtikel ini adalah studi etika kepemimpinan dengan perspektif postkolonial. Yang diteliti adalah paradigma kepemimpinan yang digunakan dalam Program Pelatihan Kepemimpinan dan Manajemen Mahasiswa(P2KMM) di Universitas Kristen Duta Wacana. Pentingnya penilaian terhadap paradigma kepemimpinan terletak pada peran menentukan dari paradigm kepemimpinan dalam keberhasilan atau kegagalan etisseorang pemimpin. Menggunakan pendekatan poskolonial, artikel ini menyoroti serangkaian nilai-nilai moral yang diakui sebagai ‘Nilai-nilai Duta Wacana,’ khususnya nilai ‘Melayani Dunia.’ Sebagai bagian dari penelitian, dilakukan observasi terhadap materi P2KMM UKDW tahun 2017 dan wawancara terhadap perwakilan tim penyusun materi dan mahasiswa pendamping P2KMM. Temuan penelitian ini menunjukkan bahwa ‘Melayani Sesama,’ cenderung terabaikan dalam program pembentukan kepemimpinan mahasiswa UKDW.
Gereja Lintas Denominasi: Membaca Narasi Kekerasan dalam Yosua 8
Authors: Listijabudi Daniel Kurniawan , Yudhita Rena Sesaria 
Published: April 2020

Abstract
The concern of this article is the fact that the Bible, especially the Old Testament, contains accounts of violence. The book of Joshua chapter 8 is one of the best examples. This article uses the method of communitarian reading to interpret that text. The event where the LORD actively commands approves Joshua to do violence to humans and other creatures is an interesting research point. Learning from the academic discourse about violence, this article observes how several congregations read the biblical text which is full of violence through so-called reader responses criticism tabulated based on some spiritual-denominational values or theological premises within a communitarian reading of four churches (Mennonite, Pentecostal-Charismatic, Calvinist, and Catholic) in their clusters.
Abstrak
Penulisan dalam artikel ini secara kritis didasari oleh kenyataan bahwa teks-teks Alkitab khususnya di dalam Perjanjian Lama, memuat narasinarasi mengenai kekerasan. Salah satu contohnya adalah teks Yosua pasal 8, yang menjadi pusat pembacaan komunitarian dalam penelitian ini. Semakin menarik perhatian untuk diteliti bahwa di dalam teks-teks yang memuat narasi kekerasan tersebut, pihak Tuhan digambarkan merestui bahkan secara aktif memerintahkan umat-Nya (dalam hal ini bangsa Israel) untuk melakukan tindakan kekerasan kepada sesama manusia, maupun ciptaan yang lain. Berdasarkan hal itulah, maka setelah memertimbangkan diskursus kekerasan dari para ahli, penelitian ini mengobservasi bagaimana jemaat-jemaat membaca teksAlkitab yang memuat narasi kekerasan dengan menggunakan metode pembacaan respon pembaca (reader response criticism) yang ditabulasikan berdasarkan nilai-nilai spiritual denominasional dari empat gereja yangmewakili komunitas yang terpilih (Mennonite, Pantekostalit-Kharismatik, Calvinis, dan Katholik) di wilayah Yogyakarta melalui interaksi per cluster secara komunitarian.
Peroxynitrite-Mediated Structural Changes in Histone H2A: Biochemical and Biophysical Analysis
Authors: Khan Md. Asad , Akram Md. Faiz , Alam Khursheed , Ahsan Haseeb , Rizvi Moshahid A. 
Published: April 2020

Background:
Peroxynitrite, a nitrating and oxidizing agent, is formed by the interaction
between nitric oxide and superoxide radicals. H2A histone is a basic nucleoprotein and is one of
the major core histones responsible for packaging DNA. It has been shown that they are highly sensitive
to oxidizing and nitrating agents.

Objective:
Nitration of tyrosine residues in proteins by peroxynitrite is regarded as a marker of
nitrosative damage. The dityrosine bond, an oxidative covalent cross-link between two tyrosines in
protein, is increasingly identified as a marker of oxidative stress, aging and neurodegerative
diseases.

Methods:
Peroxinitrite-mediated nitration and dinitration in H2A histone was assessed by various
biophysical techniques.

Results:
The data presented in this study showed that the dityrosine content was found to be elevated
in H2A histone modified with peroxynitrite. The formation of dityrosine showed a decrease in
fluorescence intensity, generation of a new peak in FT-IR, increase in hydrodynamic size, and loss
of secondary and tertiary structure of H2A resulting in a partially folded structure.

Conclusion:
We report that H2A may undergo conformational and structural changes under nitrosative
and oxidative stress from the deleterious effects of peroxynitrite.
Moxifloxacin Hydrochloride-Loaded Eudragit® RL 100 and Kollidon® SR Based Nanoparticles: Formulation, In vitro Characterization and Cytotoxicity
Authors: Kırımlıoğlu Gülsel Yurtdaş , Özer Sinan , Büyükköroğlu Gülay , Yazan Yasemin 
Published: April 2020

Background:
Considering the low ocular bioavailability of conventional formulations
used for ocular bacterial infection treatment, there is a need to design efficient novel drug delivery
systems that may enhance precorneal retention time and corneal permeability.

Aim and Objective:
The current research focuses on developing nanosized and non-toxic Eudragit®
RL 100 and Kollidon® SR nanoparticles loaded with moxifloxacin hydrochloride (MOX) for its
prolonged release to be promising for effective ocular delivery.

Methods:
In this study, MOX incorporation was carried out by spray drying method aiming ocular
delivery. In vitro characteristics were evaluated in detail with different methods.

Results:
MOX was successfully incorporated into Eudragit® RL 100 and Kollidon® SR polymeric
nanoparticles by a spray-drying process. Particle size, zeta potential, entrapment efficiency,
particle morphology, thermal, FTIR, NMR analyses and MOX quantification using HPLC method
were carried out to evaluate the nanoparticles prepared. MOX loaded nanoparticles demonstrated
nanosized and spherical shape while in vitro release studies demonstrated modified-release pattern,
which followed the Korsmeyer-Peppas kinetic model. Following the successful incorporation of
MOX into the nanoparticles, the formulation (MOX: Eudragit® RL 100, 1:5) (ERL-MOX 2) was
selected for further studies because of its better characteristics like cationic zeta potential, smaller
particle size, narrow size distribution and more uniform prolonged release pattern. Moreover, ERLMOX
2 formulation remained stable for 3 months and demonstrated higher cell viability values for
MOX.

Conclusion:
In vitro characterization analyses showed that non-toxic, nano-sized and cationic
ERL-MOX 2 formulation has the potential of enhancing ocular bioavailability.
Scaffold-based Screening and Molecular Dynamics Simulation Study to Identify Two Structurally Related Phenolic Compounds as Potent MMP1 Inhibitors
Authors: Patra Swagata , Saravanan Parameswaran , Das Bhaskar , Subramanian Venkatesan , Patra Sanjukta 
Published: April 2020

Background:
Matrix metalloproteinase 1 are zinc-dependent endopeptidases responsible
for the controlled breakdown of the extracellular matrix resulting in the maintenance of
homeostasis. Dysregulation of MMP1 leads to the progression of various pathological conditions
like cancer, rheumatoid arthritis, cardiovascular disease, skin damage and fibrotic disorder. Thus,
MMP1 inhibition is the potential drug target of many synthetic MMP1 inhibitors but lack of
substrate specificity hinders their clinical applicability. Hence, inhibitors from natural products
have gained widespread attention.

Objective:
The present study attempts screening of novel MMP1 inhibitors from the ZINC
database based on experimentally reported natural inhibitors of MMP1 as a scaffold.

Methods:
Molecular docking study was performed with 19 experimentally reported natural
inhibitors spanning across nine different classes followed by virtual screening using the selected
compounds. The selected compounds were subjected to molecular dynamics simulation.

Results:
Twenty compounds were screened with a cut-off of -9.0 kcal/mol of predicted free energy
of binding, which further converged to 6 hits after docking studies. After comparing the docking
result of 6 screened hits, two best compounds were selected. ZINC02436922 had the best
interaction with six hydrogen bond formation to a relatively confined region in the S1’site of
MMP1 and -10.01 kcal/mol of predicted free energy of binding. ZINC03075557 was the secondbest
compound with -9.57 kcal/mol predicted binding free energy. Molecular dynamics simulation
of ZINC02436922 and ZINC03075557 corroborates docking study.

Conclusion:
This study indicated phenolic compounds ZINC02436922 and ZINC03075557 as
potential MMP1 inhibitors.