bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prognostic implications of CDC45 expression in hepatocellular carcinoma
Authors: Yang Chen , Xie Shufang , Wu Yi , Ru Guoqing , He Xianglei , Pan Hongyin , Wang Shibing , Tong Xiangmin 
Published: June 2020

Abstract
Background

The outcomes of hepatocellular carcinoma (HCC) remain poor despite dramatic improvements in treatment, and novel prognostic biomarkers are urgently needed to ameliorate the situation. Cell division cycle protein 45 (CDC45) plays a key role in DNA replication, which is considered to be involved in tumorigenesis and has become a new prognostic marker. This study investigated CDC45 expression in tumour tissues and defined its prognostic value in HCC patients.
Methods

Differential transcriptional and proteomic expression profiles were obtained and validated using multiple datasets. We then used immunohistochemistry (IHC) staining to examine the expression of CDC45 in tumour tissue specimens and compare them with adjacent normal tissue specimens using a constructed tissue microarray (TMA) and analysed the relationship of clinical features and prognosis in HCC patients. Functional enrichment analyses were used to describe significantly involved hallmark pathways of differentially expressed genes (DEGs).
Results

Contrary to the transcriptome expression level in the database, our results showed that the proteome expression of CDC45 in was evidently downregulated in HCC compared with normal adjacent tissues (P < 0.0001). Although we did not find any differences in terms of vascular invasion, Edmondson grade, lymphatic infiltration, or metastasis between patients with high and low CDC45 expression in our limited number of HCC samples, low expression of CDC45 was significantly correlated with aggressive characteristics, including microvascular invasion (P = 0.046). In addition, a multivariate analysis indicated that CDC45 expression (P = 0.035) was an independent prognostic factor for the overall survival (OS) rate of patients with HCC. Furthermore, patients with low CDC45 expression levels were significantly correlated with inferior OS rates than patients with high CDC45 expression levels (P < 0.05). Functional annotations indicated that CDC45 is involved in the most significant pathways, including the cell cycle, DNA replication, drug metabolism − cytochrome P450, metabolism of xenobiotics by cytochrome P450, and chemical carcinogenesis pathways.
Conclusions

Our findings showed that a low protein level of CDC45 was associated with a poor prognosis in HCC patients, indicating that CDC45 might be a novel prognostic marker and help identify new therapeutic targets for HCC.
WITHDRAWN: Positron Emission Tomographic imaging of tumor cell death using Zirconium-89-labeled APOMAB® Following Cisplatin Chemotherapy in Lung and Ovarian Cancer Xenograft Models
Authors: Liapis Vasilios , Tieu William , Wittwer Nicole , Gargett Tessa , Evdokiou Andreas , Takhar Prab , Rudd Stacey E. , Donnelly Paul S. , Brown Michael P. , Staudacher Alexander H. 
Published: June 2020

Abstract
Purpose. There are no currently approved non-invasive methods for detecting tumor treatment responses within the first few days of treatment. The monoclonal antibody, DAB4, or its chimeric derivative, chDAB4 (APOMAB®), targets the Lupus-associated or Sjögren Syndrome-B antigen (La/SSB). La/SSB is over-expressed in malignancy and is selectively targeted by chDAB4 in cancer cells dying after DNA-damaging treatment. Therefore, chDAB4 is a unique diagnostic tool that specifically detects dead cancer cells and could be used to distinguish between chemotherapy responsive and non-responsive patients. In this study, we performed preclinical validation studies using whole-body Positron-Emission Tomography (PET) to examine tumor and normal tissue uptake of 89Zr-labeled chDAB4 in lung or ovarian tumor-bearing mice, which were left untreated or given cisplatin chemotherapy.Methods. The binding of chDAB4 and its conjugates to dead cisplatin-treated human lung and ovarian cancer cells was assessed in vitro as well as its Fc-dependent effector functions. Mice bearing xenografts of H460 lung cancer or A2780 ovarian cancer cells were untreated or given cisplatin chemotherapy followed 24 hours later by 89Zr-labeled chDAB4. Post-cisplatin tumor responses were monitored using bioluminescence imaging and caliper measurements and 89Zr-labeled chDAB4 tumor uptake was measured using an Albira SI PET imager and PMOD analysis software. On completion of experiments, organs were dissected and biodistribution of 89Zr-labeled chDAB4 was measured using a Hidex gamma-counter.Results. The chDAB4 antibody bound only to dead A2780 and H460 cells, and its binding increased with cisplatin treatment in vitro. The chDAB4 antibody did not exhibit Fc-dependent effector functions. Chemotherapy significantly increased uptake of 89Zr-labeled chDAB4 in tumors but not in normal tissues for each tumor model. The greatest differences in average uptake of 89Zr-labeled chDAB4 in subcutaneous tumors were observed 3 days post-cisplatin chemotherapy compared to untreated mice, and before tumor shrinkage was evident.Conclusion. After administration of cisplatin chemotherapy, tumor xenograft uptake of 89Zr-labeled chDAB4 was detected in vivo by PET imaging. Given that the chDAB4 mAb lacked effector activity and that malignant rather than normal tissues were targeted after chemotherapy, these results support clinical development of chDAB4 as both a predictive marker of chemotherapy response and a theranostic imaging agent, which may guide subsequent delivery of chDAB4-directed antibody drug or radio-conjugate anticancer therapies.
Outpatient vs. Inpatient Hip Arthroplasty: A Matched Case-Control Study on 90-Day Complication Rate and 2-Year Patient Reported Outcomes
Authors: Rosinsky Philip J. , Chen Sarah L. , Yelton Mitchell J. , Lall Ajay C. , Maldonado David R. , Shapira Jacob , Meghpara Mitchell B , Domb Benjamin G 
Published: June 2020

Abstract
Background
The transition to outpatient-based surgery is a major development occurring in recent years in the field of total hip arthroplasty (THA). The effect of this transition on patient reported outcomes (PROs) is still not well established. The purpose of the current study was to compare patients undergoing inpatient THA (iTHA) to patients undergoing outpatient THA (oTHA) regarding (1) perioperative variables including: surgical time, blood loss, and length of stay (2) 90-day complication rates and unplanned emergency room or office visits (3) 2-year PROs including: modified Harris hip score (mHHS), Harris hip score (HHS), forgotten joint score (FJS), pain, and satisfaction, as well as quality of live measures.
Methods
The XXX registry was analyzed for patients undergoing THA between July 2014 and April 2016. The first 100 patients undergoing oTHA were selected and matched to 100 patients undergoing iTHA via propensity matching based on the following variables: age, sex, body mass index (BMI), Charlson comorbidity index (CCI), and smoking status. The primary outcomes were PROs at two years post-operatively. The secondary outcomes were perioperative surgical variables, 90-day complication rates, and unplanned emergency and clinic visits.
Results
After exclusions, 91 patients remained in each group and were compared. The oTHA group showed improved 2-year PROs with regard to mHHS (91.5 vs. 86.2; P = 0.02), HHS (92.3 vs. 87.4; P = 0.02), and pain (1.0 vs. 1.5; P = 0.04). The oTHA group had an average length of stay of 6.8 hours compared to 43.2 hours for the iTHA group (P < 0.001). There were no significant differences between the groups regarding readmissions, emergency room visits, and unplanned clinic visits. Complications and revision rates were similar in both groups.
Conclusion
In appropriately selected patients, oTHA can achieve improved postoperative 2-year PROs compared to iTHA. We found no differences regarding postoperative short-term complications or two-year revision rates, and no differences in unplanned office visits or readmissions.
Correlation between percutaneous transthoracic needle biopsy and recurrence in stage I lung cancer: a systematic review and meta-analysis
Authors: Li Haichao , Chen Rong , Zhao Jian 
Published: June 2020

Abstract
Objective To systematically evaluate the correlation between percutaneous transthoracic needle biopsy (PTNB) and recurrence in stage I lung cancer. Methods The databases of PubMed, EMbase, The Cochrane Library, CNKI, WanFang Data and China Biology Medicine disc were retrieved to collect relevant literatures about the correlation between percutaneous transthoracic needle biopsy(PTNB) and recurrence in stage I lung cancer. The retrieval time was limited from the time of their database establishment to April 30/2020.Screened the literature, extracted the data and assessed the quality of studies included. Then the meta-analysis was performed by using Stata l6.0 software. Results A total of 8 cohort studies involving 2760 lung cancer patients were included. The results of meta-analysis showed that PTNB did not increase the risk of total recurrence and pleural recurrence in the patients with stage I lung cancer.The result of subgroup analysis is according to the tumor location. For stage I lung cancer, PTNB will increase the risk of pleural recurrence in patients with sub-pleural lesions but not in those without sub-pleural lesions. Conclusion To stage I lung cancer, PTNB is not associated with the total recurrence and pleural recurrence but PTNB will increase the risk of pleural recurrence in patients with sub-pleural lesions.
Case Report of Diaphragmatic Hernia with Small Hernia Sac Orifice
Authors: Wang Yun-jin , Chen Liu , Zhou Chao-ming , zhang zhengmian 
Published: June 2020

Abstract
Background：Congenital diaphragmatic hernia(CDH) is an embryonic stage in which the abdominal organs enter the thoracic cavity under the action of pressure difference between the thoracic and abdominal cavity, resulting in pulmonary hypoplasia due to the dysplasia of the diaphragm and the failure to heal the posterolateral pleura and peritoneum during the development of the diaphragm.Case presentation: A 7-month-old girl was admitted to hospital with the chief complaint of repeated vomiting for 1 day. Diagnosis was established by chest and abdominal radiographs and computerized tomography(CT). During the operation, it was found that there were many contents of intrathoracic hernia, and the hernia sac orifice was small. It was difficult to return them by thoracoscopy,then they were transferred to laparoscopic-assisted content return of diaphragmatic hernia.The patient had a good postoperative result.Conclusion: This is a very rare case of Congenital diaphragmatic hernia with small hernia sac orifice, and it emphasizes the significance of preoperative computerized tomography.
Improvement of Thick Coal Seam Gas Drainage Efficiency Using Highly Pressurized Multi-Discharge CO2 Gas Fracturing Technique
Authors: Lu Tingkan , Kaijage Dickson , Li Dongyin , Yang Xuelin 
Published: June 2020

Abstract
Thick coal seam fracture stimulations were carried out to improve pre gas drainage efficiency using the highly pressurized multi-discharge carbon dioxide gas fracturing technique. The paper presents research results obtained from field work and numerical simulation. The field data demonstrates that the multi-discharge fracturing technique can effectively enhance thick coal seam permeability, so as to improve thick coal seam gas drainage efficiency. On the other hand, regardless of numbers of discharge set used, the effective gas drainage radius remains unchanged, which is inconsistent with the traditional coal seam fracture stimulation, so the causes are discussed in detail. Numerical simulation indicates that the technique provides two basic functions in improving efficiency of coal seam gas drainage: 1) improving coal seam permeability and 2) forming gas pressure gradient of coal seam. The former creates fracture network of coal seam, and the latter, as a driving force, drives coal seam gas from a fracturing borehole to the drainage boreholes through the network developed.
Primary or interval debulking surgery for stage IV ovarian cancer patients: experience from a ten-year case series
Authors: Deng Qiuling , Feng Zheng , Wen Hao , Ju Xingzhu , Chen Xiaojun , Bi Rui , Wu Xiaohua 
Published: June 2020

Abstract
Background

Whether patients with stage IV ovarian cancer can benefit from neoadjuvant chemotherapy (NACT) is unclear. The main aim was to compare the survival outcomes of primary debulking surgery (PDS) versus NACT followed by interval debulking surgery (IDS) in stage IV ovarian cancer patients.
Methods

This study retrospectively analyzed 190 consecutive patients with stage IV ovarian cancer who underwent either PDS or NACT + IDS between June 2003 and June 2013 at Fudan University Shanghai Cancer Center. Progression free survival (PFS) and overall survival (OS) were analyzed with Kaplan-Meier method. Multivariable cox proportional hazard models tested for associations of potential explanatory variables with survival.
Results

A total of 111 patients underwent PDS and 79 patients received NACT + IDS. More NACT patients had stage IVB disease compared with PDS patients (p = 0.001). Additionally, a higher rate of complete resection (45.6% vs 21.6%, p < 0.001) was achieved in NACT patients compared to those with PDS. There was no significant difference of platinum sensitivity between the two groups (p = 0.998). The median PFS for PDS and NACT groups were 14 (95%CI, 12–16) and 16 (95%CI, 13–17) months, respectively (p = 0.744). The median OS were 40 (95%CI, 31–49) and 53(95% CI, 41–65) months for PDS and NACT groups, respectively (p = 0.094). In addition, NACT was more beneficial than PDS in patients with extra-abdominal lymph node metastases (median OS (95%CI) = 37 (24–50) vs 60 (41–79) months, p = 0.006).
Conclusions

There were no significant survival differences between PDS and NACT groups for stage IV ovarian cancer patients. However, NACT had survival benefit in patients with extra-abdominal lymph node metastases.
Clinical Investigations External Validation of Multimodal Termination of Resuscitation Rules for Out-of-hospital Cardiac Arrest Patients in the Covid-19 Era
Authors: Jung Haewon , Lee Mijin , Cho Jae Wan , Lee Sang Hun , Lee Suk Hee , Mun You Ho , Chung Han-sol , Kim Yang Hun , Kim Gyun Moo , Park Sin-youl , Jeon Jae Cheon , Kim Changho 
Published: June 2020

Abstract
Background:Futile resuscitation for out-of-hospital cardiac arrest (OHCA) patients in the COVID-19 era can lead to risk of disease transmission and unnecessary transport. Various existing basic or advanced life support (BLS or ALS) rules for termination of resuscitation (TOR)have been derived and validated in North America and Asian countries. This study aimed to evaluate the external validation of these rules in predicting the survival outcomes of OHCA patients in the COVID-19 era.Methods: A multicenter observational study was performed using the WinCOVID-19 Daegu registry data collected from 18 February to 31March 2020. The outcomes of each rule were compared to the actual patient survival outcomes. The sensitivity, specificity, false positive ratio (FPR), and positive predictive value (PPV) of each TOR rule were evaluated. Results: Of the 184 OHCA patients, 170 patients,who showed cardiac arrest of presumed cardiac etiology, were enrolled. TOR was recommended for 122 patientsbased on the international BLS-TOR rule, which showed 85% specificity, 74% sensitivity, 0.8% FPR, and 99% PPV for predicting unfavorable survival outcomes. When the traditional BLS-TOR rules and KoCARC TOR rule II were applied to our registry, one patient met the TOR criteria but survived at hospital discharge. With regard to the FPR (upper limit of 95% confidence interval<5%) and PPV (>99%) criteria, only the KoCARCTOR rule I, which included a combination ofthree factors including not being witnessed by emergency medical technicians, presenting with an asystole at the scene, and not experiencing prehospital shock delivery or ROSC, was found to be superiorto all other TOR rules. Conclusion: Among the previous nine BLS and ALS TOR rules, KoCARCTOR rule I was most suitable for predicting poor survival outcomes and showed improved diagnostic performance. Further research on variations in resources and treatment protocols among facilities, regions, and cultures will be useful in determining the feasibility of TOR rules for COVID-19 patients worldwide.Trial registration: Not applicable
Determinants of Dehydration Status and Associated Risk Factors of Cholera Outbreak in Oromia, Ethiopia
Authors: Ali Endale Alemayehu , Tilahun Tsigereda , Mebrate Eshetu 
Published: June 2020

Abstract
Introductions: Cholera is a diarrheal disease caused by infection of the intestine with the gram-negative bacteria Vibrio cholera. According to updated global burden of cholera estimate 2019 in Ethiopia 68,805,272 populations are at risk of cholera with incidence rate of 4 per 1000 population and case fatality of 3.8% estimated annual number of cases 275,221.Methods: The main objective of this study is to identify the significant risk factors of dehydration status of cholera outbreak in Oromia regional state of Ethiopia. Ordinal logistic regression was used to model the data by incorporating the assumption behind this novel model. Results: The results of the study indicated that of the total 965 cholera patients, most of them 560(58%) were severely dehydrated by cholera. The overall goodness of model (p-valu=0.07) shows that the model fits the data well. Besides, the proportional odds assumption also revealed that the slop coefficients in the model are the same across dehydration status (p-value=0.094). For those have history of travel, the odds of severely dehydrated versus the combined some dehydrated and no dehydrated was exp(1.133804)=3.11 times higher than those have no history of travel (p-value<0.01). All the other factors like history of contact with other patients, other sick patients in the family, Intravenous and Antibiotics drugs are statistically significant with 5% level of significance to determine the status of dehydration. Conclusions: The ordinal logistic regression was fitted the data well and most of the included factors were significant for the dehydration status of cholera outbreak.
The association between life satisfaction, emotional support and perceived health among Intimate Partner Violence (IPV) survivors
Authors: Hui Vivian , Constantino Rose 
Published: June 2020

Abstract
Background : Intimate partner violence (IPV) is a pressing phenomenon whose consequences are associated with severe physical and mental health outcomes. Every minute, around 24 people in the United States are raped, physically harmed, or emotionally abused by their intimate partner. Although having experienced IPV is not modifiable, emotional support is a protective factor to prevent victims from committing suicide. The psychological state of IPV victims is critical in post-traumatic events and this is evidenced in numerous qualitative interviews. Therefore, The objective of this study is to identify IPV and socio-demographic factors associated with emotional support, life satisfaction, and perceived health status in the United States.

Methods : This study analysed the data from 2007 Behavioral Risk Factor Surveillance System. Univariate analyses, multivariable logistic regression analyses and ordinal logistic regression analyses were used to estimate the adjusted odds ratios (AORs) and 95% confidence intervals (95% CIs) for factors associated with IPV. Analyses were conducted using SPSS version 25.

Results : The analyses show that there is a strong association between IPV experience and emotional support (AOR:1.810; 95% CI = 1.626 -2.015). Participants who had unwanted sex with an intimate partner in the past 12 months, have 2.28 higher odds to receive less emotional support and 2.05 higher odds to perceive poor life satisfaction. In addition, participants who reported experiencing IPV were associated with (AOR: 3.12; 95% CI =2.68 – 3.62) times the odds of having ≥ 6 days more mentally unhealthy days in a month. For perceived health outcomes, people who had been threatened with violence by a sex partner have 1.74 (95% CI =1.54 – 1.96) times the odds of having poor perceived general health status. IPV survivors have 3.12 (95% CI =2.68 – 3.62) times the odds of having ≥ 6 days more mentally unhealthy days in a month.

Conclusions : People reported with any IPV experience are more likely to receive less emotional support, perceive dissatisfaction in life, and poor health outcomes. This study shows the need for policies centered on the development of interventions that focus on mental health for those who have experienced IPV.
Network Pharmacology-Based Strategy for Predicting Therapy Targets of Tripterygium wilfordii on Acute Myeloid Leukemia
Authors: Fang Tingting , Liu Lanqin , liu wenjun 
Published: June 2020

Abstract
Background.
Acute myeloid leukemia (AML) is a common malignant tumor of the hematopoietic system. How to extend the survival time of AML patients and improve their prognosis is still a major medical problem. Chinese medicine has a long history in treating AML. Tripterygium wilfordii (TW) is a traditional Chinese medicine. With the deepening of pharmacological research of traditional Chinese medicine, triptolide, one of its active ingredients, has been proven to have a positive effect on the treatment of AML. Therefore,this study aimed on studying the potential therapeutic targets and pharmacological mechanism of TW in Acute myeloid leukemia (AML) based on network pharmacology.
Methods.
The active components of TW were obtained by network pharmacology through oral bioavailability, drug-likeness filtration. Comparative analysis was used to study the overlapping genes between active ingredient’s targets and AML treatment-related targets. Using STRING database to analyze interactions between overlapping genes. KEGG pathway analysis and Gene Ontology enrichment analysis were conducted in DAVID. These genes were analyzed for survival in OncoLnc database.
Results.
We screened 53 active ingredients, the results of comparative analysis showed that 8 active ingredients had an effect on AML treatment. Based on the active ingredients and overlapping genes, we constructed the Drug-Compounds-Genes-Disease Network. Survival analysis of overlapping genes indicated that some targets possess a significant influence on patients’ survival and prognosis. The enrichment analysis showed that the main pathways of targets are Toll-like receptor signaling pathway, NF-kappa B signaling pathway and HIF-1 signaling pathway.
Conclusion.
This study, using a network pharmacologic approach, provides another strategy that can help us to understand the mechanisms by which TW treats AML comprehensively.
DeepGraphMol, a multi-objective, computational strategy for generating molecules with desirable properties: a graph convolution and reinforcement learning approach
Authors: Khemchandani Yash , O'Hagan Steve , Samanta Soumitra , Swainston Neil , Roberts Timothy J , Bollegala Danushka , Kell Douglas 
Published: June 2020

Abstract
We address the problem of generating novel molecules with desired interaction properties as a multi-objective optimization problem. Interaction binding models are learned from binding data using graph convolution networks (GCNs). Since the experimentally obtained property scores are recognised as having potentially gross errors, we adopted a robust loss for the model. Combinations of these terms, including drug likeness and synthetic accessibility, are then optimized using reinforcement learning based on a graph convolution policy approach. Some of the molecules generated, while legitimate chemically, can have excellent drug-likeness scores but appear unusual. We provide an example based on the binding potency of small molecules to dopamine transporters. We extend our method successfully to use a multi-objective reward function, in this case for generating novel molecules that bind with dopamine transporters but not with those for norepinephrine. Our method should be generally applicable to the generation in silico of molecules with desirable properties.