bluuebunny/crossref_metadata_2025_split huggingface dataset:
Analysis of lymph node metastasis in early gastric cancer: a single institutional experience from China
Authors: Chen Jinggui , Zhao Guangfa , Wang Yanong 
Published: March 2020

Abstract
Background
Lymph node metastasis (LNM) has a strong influence on the prognosis of patients with early gastric cancer (EGC). The aim of this study was to reveal the incidence of LNM and evaluate risk factors for LNM to determine the appropriate treatment for EGC in a Chinese population.

Methods
Patients who underwent radical gastrectomy with lymph node dissection for EGC between 2012 and 2017 were retrospectively analyzed. Univariate and multivariate analyses were conducted to identify clinicopathological features that were risk factors for LNM.

Results
A total of 1033 patients with EGC were enrolled. Of these patients, 668 (64.7%) were men, and 365 (35.3%) were women, ranging in age from 19 to 82 years (mean 56.9 ± 10.9 years). LNM was detected in 173(16.7%) patients with EGC. Among 508 patients with mucosal cancer, 44 (8.7%) patients had LNM. In 525 patients with submucosal cancer, the incidence of LNM was 24.6% (129/525). The age, gender, tumor size, type of differentiation, Lauren classification, and lymphovascular and perineural invasion showed a significant correlation with the rate of LNM in EGC by univariate and multivariate analyses. Patients with submucosal gastric cancer had an older age, a higher proportion of proximal lesion, larger tumor size, more frequent lymphovascular invasion, perineural invasion, and more LNM than patients with mucosal gastric cancer.

Conclusions
Our study revealed a relatively high incidence of LNM in EGC, compared with Japanese and Korean cohorts. Female sex, large tumor size, undifferentiated-type, and lymphovascular invasion were independent risk factors for LNM in EGC. Radical gastrectomy with lymphadenectomy should be performed in EGC patients with a high risk of LNM.
Changing demographic trends among South African occupational therapists: 2002 to 2018
Authors: Ned Lieketseng , Tiwari Ritika , Buchanan Helen , Van Niekerk Lana , Sherry Kate , Chikte Usuf 
Published: March 2020

Abstract
Background
South Africa’s quadruple burden of disease, coupled with health system challenges and other factors, predicts a high burden of disability within the population. Human Resources for Health policy and planning need to take account of this challenge. Occupational therapists are part of the health rehabilitation team, and their supply and status in the workforce need to be better understood.

Methods
The study was a retrospective record-based review of the Health Professions Council of South Africa database from 2002 to 2018. The data obtained from the Health Professions Council of South Africa was analysed for the following variables: geographical location, population groups, age, practice type and sex. Data was entered on a Microsoft Excel spreadsheet and analysed using the Statistical Package for the Social Sciences (SPSS version 22.0).

Results
In 2018, there were 5180 occupational therapists registered with the Health Professions Council of South Africa with a ratio of 0.9 occupational therapists per 10 000 population. There has been an average annual increase of 7.1% over the time period of 2002–2018. The majority of occupational therapists are located in the more densely populated and urbanised provinces, namely Gauteng, Western Cape and KwaZulu-Natal. Most of the registered occupational therapists are under the age of 40 years (67.7%). The majority (66%) are classified as white followed by those classified as black and coloured. Females make up 95% of the registered occupational therapists. Nationally, 74.8% of occupational therapists are deployed in the private sector catering for 16% of the population while approximately 25.2% are employed in the public sector catering for 84% of the population.

Conclusions
Under-resourcing and disparities in the profile and distribution of occupational therapy human resources remain an abiding concern which negatively impacts on rehabilitation service provision and equitable health and rehabilitation outcomes.
Protocol: low cost fast and efficient generation of molecular tools for small RNA analysis
Authors: López-Márquez Diego , Del-Espino Ángel , Bejarano Eduardo R. , Beuzón Carmen R. , Ruiz-Albert Javier 
Published: March 2020

Abstract
Background
Small RNAs are sequence-dependent negative regulators of gene expression involved in many relevant plant processes such as development, genome stability, or stress response. Functional characterization of sRNAs in plants typically relies on the modification of the steady state levels of these molecules. State-of-the-art strategies to reduce plant sRNA levels include molecular tools such as Target Mimics (MIMs or TMs), Short Tandem Target Mimic (STTMs), or molecular SPONGES (SPs). Construction of these tools routinely involve many different molecular biology techniques, steps, and reagents rendering such processes expensive, time consuming, and difficult to implement, particularly high-throughput approaches.

Results
We have developed a vector and a cloning strategy that significantly reduces the number of steps required for the generation of MIMs against any given small RNA (sRNA). Our pGREEN-based binary expression vector (pGREEN-DLM100) contains the IPS1 gene from A. thaliana bisected by a ccdB cassette that is itself flanked by restriction sites for a type IIS endonuclease. Using a single digestion plus a sticky-end ligation step, the ccdB cassette that functions as a negative (counter) selection system is replaced by a pair of 28 nt self-annealing primers that provide specificity against the selected target miRNA/siRNA. The method considerably reduces the number of steps and the time required to generate the construct, minimizes the errors derived from long-range PCRs, bypasses bottlenecks derived from subcloning steps, and eliminates the need for any additional cloning technics and reagents, overall saving time and reagents.

Conclusions
Our streamlined system guarantees a low cost, fast and efficient cloning process that it can be easily implemented into high-throughput strategies, since the same digested plasmid can be used for any given sRNA. We believe this method represents a significant technical improvement on state-of-the-art methods to facilitate the characterization of functional aspects of sRNA biology.
A combination of spearmint and flaxseed extract improved endocrine and histomorphology of ovary in experimental PCOS
Authors: Mehraban Mina , Jelodar Gholamali , Rahmanifar Farhad 
Published: March 2020

AbstractBackgroundPolycystic ovary syndrome (PCOS) is a complex reproduction and endocrine disorder of women in the reproductive age. Spearmint (Mentha spicataL.) has anti-androgenic activity and flaxseed (Linum usitatissimumL.) contains phytoestrogen and was reported to improve PCOS conditions. This study aimed to evaluate PCOS conditions following administration of a mixture of these two plants.MethodsTwenty-four rats with regular cycles were randomly divided into four groups as control (C) and treatment-control (TC) received a combination of spearmint extract (SE) + flaxseed extract (FE). PCOS was induced in PCOS and treatment (T) groups by a single intramuscular injection of estradiol valerate. The treatment group received a combination of SE and FE for 30 days, 7 weeks after injection of estradiol valerate. Estrous cycles were monitored for 10 days and in the last day animals were sacrificed, ovaries were collected for the histomorphometric study and the serum levels of progesterone, testosterone, estradiol, and dehydroepiandrosterone (DHEA) were measured.ResultSignificant rise in progesterone and a decrease in testosterone and estradiol with no significant change of DHEA in the T group, was observed in comparison with the PCOS group (P < 0.05). No significant difference noticed between T and control groups (C &CT) regarding evaluated hormones. A significant increase in primary, pre-antral and antral follicles noticed in the T group compared to the PCOS group. The number of cystic follicles decreased in the T group compared with the PCOS group. Granulosa layer thickness increased while the thickness of theca decreased significantly in the T group compared to the PCOS group (P < 0.05). No significant endocrine or histological differences noticed between C and TC groups.ConclusionA combination of flaxseed and spearmint extract improved the endocrine profile and the histomorphometric features of the ovary in the T group compared to the PCOS group.
Neuromuscular exercise and pain neuroscience education compared with pain neuroscience education alone in patients with chronic pain after primary total knee arthroplasty: study protocol for the NEPNEP randomized controlled trial
Authors: Larsen Jesper Bie , Skou Søren T. , Arendt-Nielsen Lars , Simonsen Ole , Madeleine Pascal 
Published: March 2020

Abstract
Background
Total knee arthroplasty (TKA) is considered an effective treatment for pain relief and improved physical performances in end-stage knee osteoarthritis. However, several studies have reported less favorable outcomes after TKA with chronic pain rates of approximately 20%. Exercise might be an effective treatment strategy for chronic pain following TKA, but no randomized controlled trials have evaluated its effect. Therefore, the purpose of this randomized controlled trial is to investigate whether a 12-week neuromuscular exercise (NEuroMuscular EXercise training program for patients with knee or hip osteoarthritis assigned for total joint replacement; NEMEX-TJR) program combined with pain neuroscience education (PNE) provides greater pain relief and improvement in physical performances than PNE alone at 12 months follow-up in a population of patients with chronic pain after primary TKA.

Methods
For this randomized controlled superiority trial, 120 patients with moderate-to-severe chronic pain after TKA are recruited from Aalborg University Hospital, Denmark. Patients are randomly assigned in a 1:1 ratio to one of two interventions: (a) NEMEX-TJR twice weekly for 12 weeks combined with two sessions of PNE or (b) two sessions of PNE given over 6 weeks. Assessment is performed at baseline before intervention and at 3, 6, and 12 months after initiation of the intervention. Outcome assessors are blinded toward group allocation. The primary outcome is the change in the Knee Injury and Osteoarthritis Outcome Score4 (KOOS4), defined as the mean score for the KOOS subscales pain, symptoms, activities of daily living, and quality of life. Secondary outcomes include all KOOS subscale scores and scores for PainDETECT, the Fear-Avoidance Beliefs Questionnaire, Global Perceived Effect, the Pain Catastrophizing Scale, pain intensities, temporal summation, conditioned pain modulation, and pressure pain thresholds. Physical performances are measured with walking, stair climbing, and chair standing tests as well as tests of muscle strength and power.

Discussion
The findings will be useful in establishing effective treatment strategies for chronic pain after TKA. The randomized controlled trial involves rigorous scientific methods and uses clinically applicable interventions. The study interventions are conducted in clinical settings, thereby enhancing the possibility of future implementation of the treatments in the health care systems.

Trial registration
ClinicalTrials.gov identifier: NCT03886259. Registered 22 March 2019. Ethics committee registration: N-20180046.
Wrist deformity, bother and function following wrist fracture in the elderly
Authors: Lawson Andrew , Santhakumar Partiban , Naylor Justine M. , Churches Tim , Frost Steve , Harris Ian A. 
Published: March 2020

Abstract
Objective
Wrist deformity in older people is common following treatment for a wrist fracture, particularly after non-surgical treatment. A cohort of older wrist fracture patients were surveyed by telephone regarding perceived deformity, bother with deformity and patient-reported wrist function. The objectives were to: (1) determine whether older patients with wrist fractures perceived a deformity of their wrist and if they were bothered by it; (2) test if there were associations between deformity and treatment-type and between deformity and function; (3) test for associations between bother and treatment-type and between bother and function; (4) measure the test–retest reliability of the ‘bother’ question.

Results
Of 98 eligible patients who were invited to participate, 41 responded. Out of 41, 14 (34%) believed they had a deformity and 4 (10%) reported that they were bothered by the appearance of their wrist. Deformity was associated with non-surgical treatment (RR = 3.85, p = 0.006) but was not significantly associated with functional outcomes (p = 0.15). All those who were bothered belonged to the non-surgical treatment group. Bother was significantly associated with poorer functional outcomes (p = 0.006) and this association was clinically significant (MD = 35 points). The deformity and bother questions were found to have excellent test–retest reliability; κ = 1.00 and κ = 0.92, respectively.
Improved detection of esp, hyl, asa1, gelE, cylA virulence genes among clinical isolates of Enterococci
Authors: Kiruthiga Alexander , Padmavathy Kesavaram , Shabana Praveen , Naveenkumar Venkatesan , Gnanadesikan Sumathi , Malaiyan Jeevan 
Published: March 2020

Abstract
Objective
Virulence factors (VFs) among the clinical strains of enterococci play a vital role in pathogenesis. This study was aimed to screen for cylA, asa1, gelE, esp and hyl among Enterococcus faecalis (n = 89) and E. faecium (n = 51) by multiplex PCR. The previously reported multiplex PCR was modified to 2 duplex (asa1 and gelE, cylA and esp) PCRs and 1 simplex (hyl) PCR. The idea of the modification of the multiplex PCR proposed here emerged in the course of the research study when majority of the isolates which phenotypically exhibited virulence traits were found to be negative for the respective gene.

Results
cylA, gelE and asa1 were significantly predominant in E. faecalis (59.55%, 85.39%, 86.51%) than E. faecium (1.96%, 60.78%, 9.80%) (p < 0.0001, p = 0.001967, p < 0.0001). hyl was detected in E. faecium (5.9%) only. The number of VFs detected in each isolate was recorded as the VF score. E. faecalis isolates had a VF score pattern of score 4 (34.83%), score 3 (26.96%), score 2 (28.08%) and score 1 (8.98%) while E. faecium had score 4 (1.96%), score 3 (7.84%), score 2 (25.49%) and score 1 (41.18%). This modification of the PCR protocol could resolve the problem of decreased detection of virulence determinants in enterococci.
Anserine and glucosamine supplementation attenuates the levels of inflammatory markers in rats with rheumatoid arthritis
Authors: Zhao Jiang Minghao , Chen Xiang , Cheng Ke , Shi Qingming , Peng Kun 
Published: March 2020

AbstractRheumatoid arthritis (RA) is an autoimmune disorder that affects the joint synovium. Anserine is a functional dipeptide containing methylhistidine and β-alanine, and is present in the brain and skeletal muscle of birds and mammals. Glucosamine is an amino sugar used in the synthesis of glycosylated proteins and lipids. We evaluated the effects of anserine and glucosamine on RA. Rats were assigned into the control group, RA group, anserine group (1 mg/kg), glucosamine group (200 mg/kg), or anserine plus glucosamine group (anserine, 1 mg/kg + glucosamine, 200 mg/kg). Treatment was continued for 45 consecutive days and was administered orally. The serum levels of catalase, glutathione peroxidase (Gpx), superoxide dismutase (SOD), reduced glutathione (GSH), lipid peroxidation, uric acid, nitric oxide, ceruloplasmin, zinc, copper, prostaglandin E2 (PGE2), matrix metalloproteinase (MMP)-3, tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 were assayed. The mRNA and protein levels of nuclear factor (NF)-κB and inducible nitric oxide synthase (iNOS) in synovial tissue were also determined. Anserine plus glucosamine significantly increased the catalase, SOD, Gpx, GSH, and zinc levels compared to the control, anserine, and glucosamine groups. Also, anserine plus glucosamine significantly reduced the PGE2, MMP-3, TNF-α, IL-1β, and IL-6 levels compared to the control, anserine, and glucosamine groups. Furthermore, anserine plus glucosamine significantly reduced the mRNA and protein levels of NF-κB and iNOS compared to the control, anserine, and glucosamine groups. Therefore, supplementation of anserine plus glucosamine shows therapeutic potential for RA.
Multiple solutions for fractional p-Laplace equation with concave-convex nonlinearities
Authors: Chen Qiang , Chen Caisheng , Shi Yanling 
Published: March 2020

AbstractIn this paper, we investigate the existence of solutions for the fractional p-Laplace equation $$ (-\Delta)_{p}^{s}u+V(x) \vert u \vert ^{p-2}u=h_{1}(x) \vert u \vert ^{q-2}u+h_{2}(x) \vert u \vert ^{r-2}u \quad \mbox{in } \mathbb{R}^{N}, $$(−Δ)psu+V(x)|u|p−2u=h1(x)|u|q−2u+h2(x)|u|r−2uin RN, where $N>sp$N>sp, $0< s<1<p$0<s<1<p, $1< q< p< r< p_{s}^{*}:=\frac{Np}{N-sp}$1<q<p<r<ps∗:=NpN−sp, and the potential function $V(x)>0$V(x)>0 and $h_{1}(x)$h1(x), $h_{2}(x)$h2(x) are allowed to change sign in $\mathbb {R}^{N}$RN. By using variant fountain theorem, we prove that the above equation admits infinitely many small and high energy solutions.
Simulation of advection–diffusion–dispersion equations based on a composite time discretization scheme
Authors: Bu Sunyoung , Bak Soyoon 
Published: March 2020

AbstractIn this work, we develop a high-order composite time discretization scheme based on classical collocation and integral deferred correction methods in a backward semi-Lagrangian framework (BSL) to simulate nonlinear advection–diffusion–dispersion problems. The third-order backward differentiation formula and fourth-order finite difference schemes are used in temporal and spatial discretizations, respectively. Additionally, to evaluate function values at non-grid points in BSL, the constrained interpolation profile method is used. Several numerical experiments demonstrate the efficiency of the proposed techniques in terms of accuracy and computation costs, compare with existing departure traceback schemes.
Disinfection exhibits systematic impacts on the drinking water microbiome
Authors: Dai Zihan , Sevillano-Rivera Maria C. , Calus Szymon T. , Bautista-de los Santos Q. Melina , Eren A. Murat , van der Wielen Paul W. J. J. , Ijaz Umer Z. , Pinto Ameet J. 
Published: March 2020

AbstractLimiting microbial growth during drinking water distribution is achieved either by maintaining a disinfectant residual or through nutrient limitation without using a disinfectant. The impact of these contrasting approaches on the drinking water microbiome is not systematically understood. We use genome-resolved metagenomics to compare the structure, metabolic traits, and population genomes of drinking water microbiome samples from bulk drinking water across multiple full-scale disinfected and non-disinfected drinking water systems. Microbial communities cluster at the structural- and functional potential-level based on the presence/absence of a disinfectant residual. Disinfectant residual alone explained 17 and 6.5% of the variance in structure and functional potential of the drinking water microbiome, respectively, despite including multiple drinking water systems with variable source waters and source water communities and treatment strategies. The drinking water microbiome is structurally and functionally less diverse and variable across disinfected compared to non-disinfected systems. While bacteria were the most abundant domain, archaea and eukaryota were more abundant in non-disinfected and disinfected systems, respectively. Community-level differences in functional potential were driven by enrichment of genes associated with carbon and nitrogen fixation in non-disinfected systems and γ-aminobutyrate metabolism in disinfected systems likely associated with the recycling of amino acids. Genome-level analyses for a subset of phylogenetically-related microorganisms suggests that disinfection selects for microorganisms capable of using fatty acids, presumably from microbial decay products, via the glyoxylate cycle. Overall, we find that disinfection exhibits systematic selective pressures on the drinking water microbiome and may select for microorganisms able to utilize microbial decay products originating from disinfection-inactivated microorganisms.
Impacts of ocean current–atmosphere interactions on mesoscale eddy energetics in the Kuroshio extension region
Authors: Shan Xuan , Jing Zhao , Sun Bingrong , Wu Lixin 
Published: March 2020

AbstractThe surface ocean current’s imprint on the wind stress (known as the current feedback) has a significant influence on the wind power input to the ocean. In this study, we investigate the effect of the current feedback on mesoscale eddy energetics in the Kuroshio extension region using a high-resolution (9 km) coupled regional climate model. We perform three sets of simulations: one calculates the wind stress without the surface current, one includes only the mesoscale eddy’s current and another includes the entire current in the computation. In this way, the mesoscale eddy’s current feedback can be isolated and its contribution to the entire current feedback can be assessed. The simulation results show that the mesoscale eddy’s current feedback results in negative wind power input to mesoscale eddies and reduces the surface eddy kinetic energy (EKE) by ~ 20% in the Kuroshio extension region. This negative wind power is compensated primarily by the enhanced conversion from mean flow kinetic energy (MKE) and eddy potential energy (EPE) to EKE and secondarily by the reduced pressure flux divergence and horizontal dissipation. Compared to the mesoscale eddy’s current feedback, the entire current feedback has a similar impact on EKE and the wind power on mesoscale eddies, while it affects the EKE budget differently. It weakens the MKE to EKE conversion partly due to the reduced kinetic energy input to background flows by wind. Correspondingly, the negative wind power on mesoscale eddies is primarily compensated by the enhanced EPE to EKE conversion.
Optimization of immobilized Lactobacillus pentosus cell fermentation for lactic acid production
Authors: Wang Jianfei , Huang Jiaqi , Laffend Hannah , Jiang Shaoming , Zhang Jing , Ning Yuchen , Fang Mudannan , Liu Shijie 
Published: March 2020

AbstractParametric optimization is an effective way in fermentation process to improve product yield and productivity in order to save time, space and financial resources. In this study, Box–Behnken design was applied to optimize the conditions for lactic acid production by immobilized Lactobacillus pentosus ATCC 8041 cell fermentation. Two quadratic models and response surface methodology were performed to illustrate the effect of each parameters and their interactions on the lactic acid yield and glucose consumption rate in immobilized L. pentosus ATCC 8041 cell fermentation. The maximum lactic acid yield was obtained as 0.938 ± 0.003 g/g glucose with a productivity of 2.213 ± 0.008 g/(L × h) under the optimized conditions of 2.0 mm bead diameter, 5.60 pH, 115.3 g/L initial glucose concentration, and 398.2 mg biomass (CDW) in 100 mL hydrogel. The analysis of variance indicated that the quadratic model was significant and could be used to scale up the fermentation process.