bluuebunny/crossref_metadata_2025_split huggingface dataset:
Quality of Sleep in People Living With HIV
Authors: Medeiros Rafaela Catherine da Silva Cunha de , Silva Tatiane Andreza Lima da , Santos Isis Kelly dos , Andrade Ricardo Dias de , Barboza Renata Rangel , Medeiros Jason Azevedo de , Araújo Juliany de Souza , Varela Phelipe Wilde de Alcântara , Knackfuss Maria Irany , Cabral Breno Guilherme de Araújo Tinoco , Dantas Paulo Moreira Silva 
Published: March 2020

Abstract
Background: Throughout the history of AIDS, changes in behavior patterns, sleep quality, development of depressive symptoms, low self-esteem, low levels of self-care, fear and anxiety were observed. The aim of this study is to analyze the factors associated with sleep quality in people living with HIV/AIDS (PLHA). Methods: It is a cross-sectional study with 314 PLHA. The Pittsburgh Sleep Quality Index was used to assess sleep quality. Socioeconomic, clinical and lifestyle habits were investigated through a structured anamnesis. Using the bivariate analysis, the differences between the sleep quality components in relation to the independent study variables were verified (p <0.10). Linear regression was performed following a multilevel hierarchical model for each sleep quality component (p ≤ 0.5). Results: Regarding the sleep quality domains, there was an association with socioeconomic and clinical factors. Sleep latency with marital status; usual efficiency with marital status and use of HAART and sleep disorders with schooling. Conclusions: The study concludes that sleep latency and usual efficiency in PLHA are associated with marital status, while sleep quality with gender and sleep disturbance with schooling.
Placenta-Specific Protein 1 Enhances Liver Metastatic Potential and is Associated with the PI3K/AKT/NF-κB Signaling Pathway in Colorectal Cancer
Authors: Ma Jiachi , Li Lei , Du Jun , Pan Chengwu , Zhang Chensong , Chen Yuzhong 
Published: March 2020

Abstract
Abstract
Background: To better explore the underlying mechanism of liver metastatic formation by placenta-specific protein 1 (PLAC1) in human colorectal cancer, we investigated the proliferation, invasion and angiogenic capabilities of human colorectal cancer cell lines with different liver metastatic potentials as well as the mechanism of action of PLAC1 in the metastatic process.
Methods: The expression of PLAC1 was detected by reverse transcriptase PCR, western blot and real-time PCR. The effect of PLAC1 on metastatic potential was determined by proliferation, invasion, and angiogenesis assays, including an in vitro coculture system consisting of cancer cells and vascular endothelial cells that were used to detect the relationship between cancer cells and angiogenesis. In addition, we also determined PLAC1 downstream targets that preferentially contribute to the metastatic process.
Results: PLAC1 was expressed in HT-29, WiDr and CaCo-2 colorectal cancer cells but not in Colo320 colorectal cancer cells. PLAC1 not only enhanced significantly the proliferation of CoLo320 and human umbilical vein endothelial cells (HUVECs), but also promoted the invasion of CoLo320 cells. The angiogenesis of HUVECs was enhanced by PLAC1 in a dose-dependent manner. In cocultured systems, angiogenesis was significantly increased by coculture with HT-29 cells. In addition, PLAC1 could promote angiogenesis in coculture with HT-29 cells. Furthermore, PLAC1-enhanced metastatic potential of colorectal cancer cells was dependent on activation of the PI3K/Akt/NF-κB pathway.
Conclusions: The activation of PI3K/Akt/NF-κB signaling by PLAC1 may be critical for the metastasis of colorectal cancer cells. According to our results, we suggest that modification of PLAC1 function might be a promising new therapeutic approach to inhibit the aggressive spread of colorectal cancer.
Two nuclear localization signals regulate intracellular localization of the Duck enteritis virus UL13 protein
Authors: Yang Linjiang , Hu Xixia , Cheng Anchun , Wang Mingshu , Jia Renyong , Yang Qiao , Wu Ying , Chen Shun , Liu Mafeng , Zhu Dekang , Ou Xumin , Wen Xingjian , Mao Sai , Sun Di , Zhang Shaqiu , Zhao Xinxin , Huang Juan , Gao Qun , Liu Yunya , Yu Yanling , Zhang Ling , TIan Bin , Pan Leichang , Rehman Mujeeb Ur , Chen Xiaoyue 
Published: March 2020

Abstract
Background UL13 multifunctional tegument protein duck enteritis virus (DEV) is predicted as conserved herpesvirus protein kinase (CHPK); however, little is known about its subcellular localization signal. Results In this study, by transfection with two predicted nuclear signals of DEV UL13 fused to enhanced green fluorescent protein (EGFP), two bipartite nuclear localization signals (NLS) were identified. We found that the NLSs block its nuclear import using ivermectin and proved that nuclear localization signal of DEV UL13 is a classical importin α/β-dependent process. And we constructed the DEV UL13 mutant strain, with the NLSs of DEV UL13 deleted, to explore whether it can affect the virus replication Conclusions The DEV pUL13 amino acids 4 to 7 and 90 to 96 was predicted, and proved that this nuclear import occurs via the classical importin α/β-dependent process. We also found NLSs of pUL13 have no effect on DEV replication in cell culture. Our study enhances the understanding of DEV pUL13. Taken together, these results would provide significant information for the biological function of pUL13 during DEV infection.
Stigma for Common Mental Disorders in Racial Minorities and Majorities: A Systematic Review and Meta-Analysis
Authors: Eylem Ozlem , de Wit Leonore , straten Annemieke van , Steubl Lena , Melissourgaki Zaneta , Danışman Gözde Topgüloğlu , de Vries Ralph , Kerkhof Ad J.F.M , Bhui Kamaldeep , Cuijpers Pim 
Published: March 2020

Abstract
Background There is a strong stigma attached to mental disorders preventing those affected from getting psychological help. The consequences of stigma are worse for racial and/or ethnic minorities compared to racial and/or ethnic majorities since the former often experience other social adversities such as poverty and discrimination within policies and institutions. This is the first systematic review and meta-analysis summarizing the evidence on the impact of differences in mental illness stigma between racial minorities and majorities. Methods This systematic review and meta-analysis included cross-sectional studies comparing mental illness stigma between racial minorities and majorities. Systematic searches were conducted in the bibliographic databases of PubMed, PsycINFO and EMBASE until 20 th December 2018. Outcomes were extracted from published reports, and meta-analyses, and meta-regression analyses were conducted in CMA software. Results After screening 2,787 abstracts, 29 studies with 193,418 participants (N=35,836 in racial minorities) were eligible for analyses. Racial minorities showed more stigma than racial majorities (g=0.20 (95% CI: 0.12~0.27) for common mental disorders. Sensitivity analyses showed robustness of these results. Multivariate meta-regression analyses pointed to the possible moderating role of the number of studies with high risk of bias on the effect size. Racial minorities have more stigma for common mental disorders when compared with majorities. Limitations included moderate to high risk of bias, high heterogeneity, few studies in most comparisons, and the use of non-standardized outcome measures. Conclusions Mental illness stigma is higher among ethnic minorities than majorities. An important clinical implication of these findings would be to tailor anti-stigma strategies related with mental illnesses according to specific racial and/or ethnic backgrounds with the intention to improve mental health outreach.
Application of a new 68Ga-labeled cNGR dimer peptide to microPET imaging of ovarian cancer
Authors: Yang Yi , Zhang Jun , Zou Huifeng , Shen Yang , Wu Yiwei 
Published: March 2020

Abstract
Introduction: Peptides containing the asparagine-glycine-arginine (NGR) sequence have been found to specifically bind to cluster of differentiation 13 (CD13) (aminopeptidase N), a tumor neovascular biomarker that is overexpressed on the surface of angiogenic blood vessels and various tumor cells and plays an important role in angiogenesis and tumor progression. The aim of this study was to evaluate the efficacy of a gallium-68 ( 68 Ga)-labeled dimeric cyclic NGR (cNGR) peptide as a new molecular probe that binds to CD13 in vitro and in vivo .Materials and Methods: A dimeric cNGR peptide conjugated with 1,4,7,10-tetraazacyclododecane-N,N',N'',N'''-tetraacetic acid (DOTA) and DOTA-c(NGR) 2 was synthesized and labeled with 68 Ga. In vitro uptake and binding analysis was performed in two ovarian tumor cell lines, ES2 and SKOV3, each of which have different expression levels of CD13. An in vivo biodistribution study was performed in normal mice, and micro positron emission tomography (PET) imaging was performed in nude mice xenografts with ES2 and SKOV3 tumors. Results : 68 Ga-DOTA-c(NGR) 2 with high radiochemical purity (>95%) was obtained and found to be stable at room temperature and when incubated with bovine serum at 37°C for 3 h. In vitro studies showed that uptake of 68 Ga-DOTA-c(NGR) 2 in ES2 cells increased over time, was higher than that in SKOV3 cells at all time points, and could be blocked by cold DOTA-c(NGR) 2 . Biodistribution studies demonstrated that 68 Ga-DOTA-c(NGR) 2 was mainly excreted from the kidney and rapidly cleared from blood. MicroPET imaging of ES2 tumor xenografts showed that focal uptake in tumors was distinctly observed from 1 to 1.5 h post-injection of 68 Ga-DOTA-c(NGR) 2 . Clear and high-contrast tumor visualization occurred at 1 h, which corresponded to the highest tumor/background ratio of 10.30±0.26. Moreover, accumulation of the probe in ES2 tumors apparently declined with pretreatment of unlabeled peptide, which further proved the specificity of 68 Ga-DOTA-c(NGR) 2 . In SKOV3 tumor models, the tumor was not obviously displayed under the same imaging protocols. Conclusion : We conclude that 68 Ga-DOTA-c(NGR) 2 might be a potential molecular probe for evaluating the expression levels of CD13 in different tumors, thereby providing a basis for targeting angiogenesis in cancer therapy.
Heritability and correlation analysis of morphological and yield traits in genetically modified cotton
Authors: Rehman Abdul , Mustafa Nida , Xiongming Du , Azhar Muhammad Tehseen 
Published: March 2020

Abstract
Background Cotton is known for its fiber and it is grown in tropical and sub-tropical areas of the world. It has a significant role in GDP of Pakistan. Therefore, present two years research was conducted to estimate heritability and association among various yield contributing parameters of cotton. The selected genotypes of cotton were hybridized in green house of the department. The F 0 cotton seed along with parents were planted in the field conditions during May, 2018. The sowing of this experiment was completed in three replications followed by RCBD. The data was recorded at maturity for various agronomic traits including plant height, number of bolls per plant, number of sympodial branches per plant, seed cotton yield, boll weight, seed index, ginning out turn, fiber length, fiber strength, and fiber fineness. Level of significance of data was computed by ANOVA to assess the difference among cotton genotypes which was used for estimation of heritability and correlation analysis among the related traits. Results Association analysis revealed that seed cotton yield had significant positive relationship with plant height, number of bolls per plant, number of sympodial branches per plant, ginning out turn, staple length and fiber strength. Staple length and fiber strength were negatively linked with each other. Estimates of heritability were high for all observed traits except number of sympodial branches per plant and boll weight. Conclusion The parent IUB-222 was found best for plant height, number of bolls per plant, boll weight, ginning out turn, seed cotton yield and seed index. NIAB-414 and VH-367 were identified best parents for fiber length, strength and fineness. Among crosses NIAB-414 × IUB-222 was best for number of bolls per plant, seed index, seed cotton yield and fiber fineness. Whereas, cross NIAB-414 × CIM-632 was good for plant height. The combination of A555 × CIM-632 was best for number of sympodial branches per plant, boll weight, fiber length and strength. VH-367 × CIM-632 proved best for ginning out turn. The correlation results from this study would be helpful to breed cotton cultivars with good yield and quality characters. Broad sense heritability was high for all of parameters which provides the strong evidence that selection in early generations can improve the performance of these traits.
Longitudinal Microglial Activation in Tau Transgenic P301S Mice Predicts Increased Tau Accumulation and Deteriorated Spatial Learning
Authors: Eckenweber Florian , Luque Jose Medina , Blume Tanja , Sacher Christian , Biechele Gloria , Wind Karin , Deussing Maximilian , Briel Nils , Lindner Simon , Boening Guido , von Ungern-Sternberg Barbara , Unterrainer Marcus , Albert Nathalie L. , Zwergal Andreas , Levin Johannes , Bartenstein Peter , Cumming Paul , Rominger Axel , Höglinger Günter U. , Herms Jochen , Brendel Matthias 
Published: March 2020

Abstract
Background: P301S tau transgenic mice show age-dependent accumulation of neurofibrillary tangles in brainstem, hippocampus, and neocortex, leading to neuronal loss and cognitive deterioration. However, there is hitherto only sparse documentation of the role of neuroinflammation in tau mouse models. Thus, we analyzed longitudinal microglial activation by small animal 18kDa translocator protein positron-emission-tomography (TSPO µPET) imaging in vivo , in conjunction with terminal assessment of tau pathology, spatial learning, and cerebral glucose metabolism. Methods: Transgenic P301S (n=33) and wild-type (n=18) female mice were imaged by 18 F-GE-180 TSPO µPET at the ages of 1.9, 3.9 and 6.4 months. We conducted behavioral testing in the Morris water maze, 18 F-fluordesoxyglucose ( 18 F-FDG) µPET and AT8 tau immunohistochemistry at 6.3-6.7 months. Terminal microglial immunohistochemistry served for validation of TSPO µPET results in vivo, applying target regions in brainstem, cortex, cerebellum and hippocampus. We compared the results with our historical data in amyloid -β mouse models. Results: TSPO expression in all target regions of P301S mice increased exponentially from 1.9 to 6.4 months, leading to significant differences in the contrasts with wild-type mice at 6.4 months (+11-23%, all p<0.001), but the apparent microgliosis proceeded more slowly than in our experience in amyloid-β mouse models. Spatial learning and glucose metabolism of AT8-positive P301S mice were significantly impaired at 6.3/6.5 months compared to the wild-type group. Longitudinal increases in TSPO expression predicted greater tau accumulation and lesser spatial learning performance at 6.7/6.3 months. Conclusions: Monitoring of microglial activation in P301S tau transgenic mice by TSPO µPET indicates a delayed time course when compared to amyloid-β mouse models. Detrimental associations of microglial activation with outcome parameters are opposite to earlier data in amyloid-β mouse models. The contribution of microglial response to pathology accompanying amyloid-β and tau over-expression merits further investigation.
Changes of Health Related Quality of Life during Pregnancy based on Pregnancy Context: A prospective study
Authors: Kazemi Ashraf , Dadkhah Aazam , Torabi Fatemeh 
Published: March 2020

Abstract
Background: The significance of planned pregnancy is an accepted principle for improving the health of pregnant women; and quality of life, as one of the important indicators of women's health, is reduced in high-risk pregnancies. The aim of this research was to investigate the changes in the health related quality of life (HRQL) in low risk pregnancies in different groups based on pregnancy context. Methods: The present study was a longitudinal prospective study conducted on 250 pregnant women divided into three groups of women with planned pregnancy, unplanned/wanted pregnancy and unwanted pregnancy. Then, using WHOQOL-26 questionnaire, the quality of life of these women was measured in physical, psychological, social and environmental dimensions at the beginning of pregnancy as well as at the end of the first, second and third trimesters. Results: Based on the results, the mean score of environmental-HRQL in women with unwanted pregnancy was significantly lower than the other two groups. All dimensions on HRQL were influenced by time and group. However, changes in the physical, psychological and social dimensions of HRQL varied within the groups. Physical- HRQL changes were different within the groups. The intergroup effect on environmental dimension of quality of life changes was significant.Conclusion: It was observed in this study that HRQL in the women with unwanted pregnancy was lower than the women with planned pregnancy and those with unplanned /wanted pregnancy. Moreover, increase in gestational age would lower quality of life, but this decline had a similar pattern in different groups.
Evaluation of the anaesthetic theatre educational environment at the University of the Witwatersrand.
Authors: Khan Saad Ali , Perrie Helen , Scribante Juan , Green-Thompson Lionel 
Published: March 2020

Abstract
Background The educational environment impacts on various aspects of students’ professional development and well-being and is an important aspect of medical education programmes. Limited research investigating the theatre educational environment has been identified in South Africa and there is limited understanding of this environment at the University of the Witwatersrand (Wits). This study aims to describe the educational environment in theatre for registrars in the Department of Anaesthesiology at Wits. Methods A prospective, descriptive study design was followed, involving anaesthetic registrars at Wits, using the adapted Anaesthetic Theatre Educational Environment Measure (ATEEM) questionnaire between April and June 2019. A convenience sampling method was implemented to administer the anonymous questionnaire. Categorical variables were described using numbers and percentages. Descriptive statistics were used to calculate the total scores. The independent t-test or analysis of variance test was used to make comparisons. Results A total of 85 anaesthetic registrars participated in this study. The mean total (SD) adapted ATEEM score was 116.5 (16.9) out of a total of 164. The total score for male and female registrars was 118.4 (13.5) and 115.3 (18.7) respectively (p=0.419). First-year registrars scored 121.6 (14.3), second years 111.1 (19.0), third years 111.4 (14.6) and fourth years 122.3 (18.7) (p=0.003). Junior and senior registrars scored 117.1 (17.0) and 115.7 (17.1) respectively (p=0.708). The three hospitals were scored 112.3 (19.5), 119.8 (16.4) and 115.4 (12.0) respectively (p=0.187). The mean scores for the five domains were autonomy 25.4/32 (3.1), perception of atmosphere 31.6/44 (5.4), workload/supervision/support 17.3/28 (3.5), perception of teachers and teaching 14.3/20 (2.9), and learning opportunities and orientation to learning 27.9/40 (5.1). Conclusion The theatre educational environment is perceived as more positive than negative, but with room for improvement. The specific domains that require attention to further improve the educational environment were perception of atmosphere, workload/supervision/support, perception of teachers and teaching, and learning opportunities and orientation to learning. With the availability of a validated instrument to assess the educational environment objectively and quantitatively, regular quality assessments of the educational environment should be included in the training institution’s practice.
Short-course Rifaximin therapy is effective and safe in patients with Diarrhea-Predominant Irritable Bowel Syndrome
Authors: Zhuang Xiaojun , Tian Zhenyi , Luo Mei , Xiong Lishou 
Published: March 2020

Abstract
Background and Aim Gut dysbiosis has been implicated in the pathogenesis of irritable bowel syndrome (IBS). Small intestinal bacterial overgrowth (SIBO), a manifestation of gut dysbiosis, is associated with diarrhea-predominant IBS (IBS-D). Rifaximin could effectively and safely improve IBS symptoms. The aim of this study was to investigate the effect of rifaximin on Chinese IBS-D patients.Methods This study included 78 IBS-D patients defined by the Rome IV criteria. Patients received 400 mg rifaximin twice daily for 2 weeks and participated in a 10-week follow-up. Gastrointestinal (GI) symptoms, quality of life (QOL) and lactulose hydrogen breath test (LHBT) results were assessed before and after treatment. Results All participants showed significant improvements in all GI symptom subdomains after rifaximin treatment (all P < 0.05), which were maintained for at least 10 weeks of follow-up. Additionally, QOL scores were increased with concomitant improvement of clinical symptoms (all P < 0.05). The 45 rifaximin-responsive patients (57.7%) achieved significantly greater GI-symptom improvement than non-responders (all P < 0.05). No GI symptoms were associated with the presence of SIBO (all P > 0.05). SIBO normalization after rifaximin treatment measured by LHBT was found in 44.4% (20/45) of patients with SIBO before treatment. Conclusion A short course (2 weeks) of rifaximin effectively and safely improved GI symptoms and QOL in Chinese patients with IBS-D. However, it did not eradicate SIBO completely, indicating that rifaximin might not be closely associated with LHBT normalization and that an additional therapeutic mechanism of rifaximin is urgently needed.
Circ_0072995 promotes cell carcinogenesis via up-regulating miR-149-5p-mediated SHMT2 in breast cancer
Authors: Qi Chuang , Qin Xianxiong , Zhou Zuozhi , Wang Yan , Yang Qin , Liao Tianzhi 
Published: March 2020

Abstract
Background Circ_0072995 is a novel identified circRNA and has been identified to involve in the metastasis of breast cancer. However, the detailed function and mechanism of circ_0072995 in the biological property of breast cancer cell remain vague. Methods The expression of circ_0072995, microRNA (miR)-149-5p and serine hydroxymethyltransferase 2 (SHMT2) mRNA was detected using quantitative real-time polymerase chain reaction. Western blot was used to detect levels of SHMT2, hexokinase-2 (HK-2), lactate dehydrogenase a chain (LDHA), glucose transporter 1 (GLUT1) and phosphoinositide 3-kinase (PI3K)/p-protein kinase B (AKT) pathway/mammalian target of rapamycin (mTOR) pathway-related protein. Cell proliferation, apoptosis, migration, and invasion were analyzed using cell counting kit-8 assay, flow cytometry, caspase-3 activity analysis, cell adhesion assay and transwell assay, respectively. Glucose metabolism was calculated by measuring glucose uptake, lactate production, and adenosine triphosphate (ATP) levels. The interaction between miR-149-5p and circ_0072995 or SHMT2 was confirmed by dual-luciferase reporter assay. In vivo tumorigenesis was performed using the murine xenograft model. Results Circ_0072995 and SHMT2 were up-regulated in breast cancer tissues and cell lines, and knockdown of circ_0072995 or SHMT2 suppressed cell malignant properties and anaerobic glycolysis; importantly, SHMT2 overexpression attenuated the anticancer action of si-Circ_0072995 in breast cancer. Besides, we also found miR-149-5p directly bound to circ_0072995 or SHMT2 in breast cancer cells, and circ_0072995 promoted the expression of SHMT2 by competitively binding to miR-149-5p. Moreover, circ_0072995 activated PI3K/AKT/mTOR pathway via elevating SHMT2 through miR-149-5p in vitro and in vivo. Additionally, xenograft tumors analysis showed circ_0072995 silence suppressed tumor growth via regulating SHMT2 and miR-149-5p. Conclusion This study demonstrated that circ_0072995 promoted cell malignant phenotypes and anaerobic glycolysis in breast cancer via up-regulating SHMT2 through sponging miR-149-5p, and activated PI3K/AKT/mTOR pathway via miR-149-5p/ SHMT2 axis, indicating a promising molecular target for breast cancer treatment.