bluuebunny/crossref_metadata_2025_split huggingface dataset:
Demitologizacja polskiej historii w reportażach literackich Pawła Smoleńskiego
Authors: Wiszniowska Monika 
Published: June 2020

Paweł Smoleński adds an important voice to the critical discussion about Polish history. In his books such as Pochówek dla rezuna, Syrop z piołunu. Wygnani w akcji „Wisła” and Krzyżyk niespodziany. Czas Goralenvolk (written together with Bartłomiej Kuraś) the author reveals the dark side of incidents that occurred during the Second World War and soon after. With full consciousness of the stereotypes and tradition fixed in the Polish memory, shows his relation to the history of na-tion from, on the one hand, and excavates from it the essence that may and should lead to a better understanding of the present time, on the other. Using the biography of ordinary people, Smoleński, showing the full value of private, colloquial, sometimes internally discrepant voices, reconstructs the process of passing into silence, political control of reminiscences and historical falsehood. His books are stories written to warn.
First of all, it is very good literature with a universal message. Smoleński shows in a very in-teresting way the “human measure of history,” helplessness of ordinary people in the face of politics and formal institutions, the easiness of controlling the reaction of masses and power of the language of propaganda. His books open the way for a better review of difficult past times but also, what is more important, help to better understand the present.
Bağ budama artıklarının peletlenmesi ve peletlerin fiziksel özelliklerinin belirlenmesi
Authors: KOÇER Abdülkadir , KÜRKLÜ Ahmet 
Published: June 2020

Amaç: Her yıl üzüm bağlarından önemli miktarda bağ budama artıkları ortaya çıkmaktadır. Bu artıklar önemli bir enerji potansiyeline sahiptir. Bu çalışmanın amacı bu artıkları pelet olarak değerlendirmektir.
Materyal ve Yöntem: Öğütülmüş bağ budama artıkları, iki farklı nem içeriğinde (yaklaşık %11 ve %15) ve üç farklı parçacık boyutunda (2, 4, 6 mm) pelet makinesinde çevre şartlarında peletlenmiştir. Peletlerin kalitesini belirleyen hacim yoğunluğu, parça yoğunluğu, mekanik dayanıklılık direnci, basınç direnci ve nem alma durumu gibi fiziksel özellikleri belirlenmiştir. Ayrıca, pelet makinesinin üretim kapasitesi ve enerji tüketim değerleri ölçülmüştür.
Araştırma Bulguları: Üretilen peletlerin fiziksel özellikleri materyal nem içeriği ve parçacık boyutuna bağlı olarak hacim ve parça yoğunluğu ortalama 696 kg m-3 ve 1262 kg m3, mekanik dayanıklılık direnci %91 ve gerilme direnci 2.36 N bulunmuştur. Ortalama üretim kapasitesi ise 45 kg h-1 olarak ölçülmüştür.
Sonuç: %11 ve %15 nem içeriğinde bağ budama artıklarından kaliteli peletler üretildi. Bu artıklardan elde edilen peletlerin fiziksel özellikleri literatür verileri ile uyumlu olduğu tespit edilmiştir.
Reproductive Performances of Friesian Holstein Dairy Cows in Different Agricultural Ecosystems
Authors: Kusmayadi Tendy , Hadiana Muhamad Hasan , Tanuwiria Ujang Hidayat 
Published: June 2020

The objective of this study was to study reproductive performance of Friesian Holstein (FH) dairy cows in the three different agro-ecosystems at the operational area of Bayongbong’s cooperative, Garut regency, Indonesia. The method used in this study was the survey method. The study was carried out in Lebakjaya village, which has drylands and rainfed agricultural ecosystem (AES DL-Rainfed); Cintanagara village which has drylands and irrigated rice field agricultural ecosystem (AES DL-IRF); and Pamalayan village which has drylands tropical forest (AES DL-Forest). The total samples were 208 dairy farmers and 315 head of FH dairy cows from the three places, 18 dairy farmers and 44 head in Lebakjaya village, 95 dairy farmers and 120 head in Cintanegara village, and 95 dairy farmers with 151 head in Pamalayan village. The study used a simple random sampling and the reproductive performances data were obtained from the field and artificial insemination practices. The data were descriptively analyzed using SPSS 22.0. After that, it was tested using T-Student statistical analyses. The results showed that the reproductive performances in AES DL-Rainfed were S/C 2.23 ± 0.45 times, days open (DO) 148.89 ± 65.52 days, and calving interval (CI) 431.00 ± 65.18 days. The reproductive performances in AES DL-IRF were S/C 2.31 ± 0.54 times, DO 161.95 ± 61.99 days, and CI 444.47 ± 61.21 days, and the reproductive performances in AES DL-Forest were S/C 2.11 ± 0.62 times, DO 138.38 ± 47.18 days, and CI 419.86 ± 46.95 days. The reproductive performances of AES DL-Forest were more efficient (P<0.05) than the AES DL-IRF. It was concluded that the reproductive performances in the three study locations, for the characters of reproductive value S/C and DO deviated from the ideal estimation, whereas the CI was around the ideal estimation.
Development of a Database of Coagulazonrgative Staphylococcus Strains, Circulating in Hospitals of Different Profiles
Authors: Kryazhev D. V. , Belyaeva E. V. , Ermolina G. B. , Boriskina E. V. , Kolesnikova E. A. , Speranskaya E. V. , Brusnigina N. F. 
Published: June 2020

Object of development: database «Strains of coagulase-negative staphylococcus, persisting in hospitals of different profile».The purpose of the study: inclusion of modern information technologies in the system of epidemiological surveillance and monitoring of infections caused by ecovars of coagulasenegative staphylococcus circulating in Nizhny Novgorod. Methodology: source data was converted into a set of related tables and organized into a hierarchical two-tier database. The database contains information about 1045 strains, circulated in hospitals of different profile of Nizhny Novgorod. To form a sample describing of clinical, epidemiological and phenotypic characteristics of strains requests are made. As a result of the query, a table is formed, where each line corresponds to one strain and contains information about the date, source and location of the recovery, the phenotype of antibiotic and phage resistance, the presence of the mecA gene, as well as sensitivity to different classes of disinfectants and the ability to form biofilm. The database is refillable. It was created using Microsoft Office Access with a standard application package. The database includes 17 queries and 14 tables. Type of implementing computer: IBM PC-compatible PC. View and version of the database management system: Access 2002 and later. Operating system version: Windows 7 and later. The developed database allows searching and processing of epidemiologically significant information about strains of coagulaze-negative staphylococcus.The application of the database is to improve the microbiological monitoring system in epidemiological surveillance of healthcare-associated infections.
A univariable and multivariable analysis of right censored time-to-event data based on restricted mean survival time: A combination with traditional survival methods.
Authors: Huang Qiao , Lyv Jun , Li Bing-hui , Ma Lin-lu , Deng Tong , Zeng Xian-Tao 
Published: June 2020

Abstract
Background: Many traditional survival analyses and restricted mean survival time (RMST) based analyses have been proposed for dealing with right censored time-to-event data. It is necessary to sort out the conditions and relationship among these methods for instruction. Comparison between hazard ratio (HR) and RMST in a study may promote appropriate understanding and application of the two effect measures.Method : Traditional survival analyses and RMST-based analyses were integrated into a flowchart and applied for univariable and multivariable analyses, RMST-based analyses included group comparison for difference in RMST, pseudo-value (PV) regressions, inverse probability of censoring probability (IPCW) regressions with group-specific weights and individual weights with homogeneity of censoring mechanism assumption. 4405 osteosarcomas were captured from Surveillance, Epidemiology and End Results Program Database. HR and difference in RMST (survival time lost or gain, STL or STG) were considered as effect measures and the effect of all included covariates on overall survival were reported for comparison. The relationship between difference in RMST and HR were explored when proportional hazard (PH) assumption was and was not met, respectively. Results: In group comparison and univariable regressions, using difference in RMST calculated by Kaplan-Meier methods as reference, PV regressions (R2=0.99) and IPCW regressions with group-specific weights (R2=1.00) provided more consistent estimation on difference in RMST than IPCW with individual weights (R2=0.09). In multivariable analysis, age (HR:1.03, STL: 3.86 months), diagnosis in 1970~1980s (HR:1.39 STL:27.49 months), metastasis (HR:4.47, STL: 202 months), surgery (HR:0.58, SLG:35.55 months) and radiation (HR:1.46, SLT:44.65 months) met PH assumption and were main independent factors for overall survival. In both univariable and multivariable variables, a robust negative logarithmic linear relationship between HRs estimated by Cox regression and differences in RMST by pseudo-value regressions was only observed when PH assumption was hold. Conclusion: The flowchart may be intuitive and helpful to instruct appropriate use of RMST based and traditional methods. PH assumption and homogeneity of censoring mechanism assumption will determine the appropriate selection of these method. HR and difference in RMST can be reported comprehensively.
Characterization of tumor microenvironment and Immune infiltration in head and neck squamous cell carcinoma
Authors: wu zenghong , cheng qing 
Published: June 2020

Abstract
Background: Head and neck squamous cell carcinoma (HNSCC), is a frequent malignant tumor of the head and neck. Most HNSCC arise in the lip, oral cavity, paranasal sinuses, oropharynx, larynx, nasopharynx, and the pharynx. HNSCC is a heterogeneous disease entity, and the role of immune cells in HNSCC, particularly in immunotherapy has not yet been extensively studied. Method: In this study, we applied CIBERSORT to infer the infiltration of 22 subsets of TIICs using gene expression data. This was to determine the relationship of the immune subpopulation, patients’ survival, function, and expression differences to reveal potential targets and biomarkers for immunotherapy. Results: Somatic mutations were the most common and the variant classification was missense mutation. The most common DNA sequence polymorphism type was SNP and the most common single nucleotide variants (SNV) class was C>T. The median number of variants per sample was 78 in HNSCC patients. The top 10 mutated genes related to TMB were TP53, TTN, FAT1, MUC16, CDKN2A, CSMD3, SYNE1, LRP1B, NOTCH1, and PIK3CA. We portrayed the immune scene in detail, uncovering the awesome immune infiltration styles of various subtypes in HNSCC. Conclusion: The intricate connection between TIIC, TMB, and genomic alterations was additionally set up. Our paintings advance the information of immune response and offer significant assets for research to enhance immunotherapy.
Both A-to-G and C-to-T base editing in human cells with dual base editor (A&amp;C-BEmax)
Authors: Zhu Biyun , Zhang Xiaohui , Chen Liang , Wang Liren , Li Dali 
Published: June 2020

Abstract
As promising genome editing tools, cytidine (CBEs) and adenosine base editors (ABEs) efficiently catalyze C•G to T•A and A•T to G•C conversions, respectively. However, CBEs or ABEs only generate single type of nucleotide conversions, which limits the sequence diversity of edited site and therapeutic applications at certain circumstances. A dual base editor(A&C-BEmax) has been developed to achieve both C•G to T•A and A•T to G•C mutations through fusion of cytidine and adenosine deaminases to Cas9n. In this protocol, we demonstrate the generation of diverse point mutations, including a simultaneous -113A-to-G and -114C-to-T conversions, in the hemoglobin gamma (HBG) gene promoter region in HUDEP-2 cells through A&C-BEmax with a single sgRNA, leading to reactivation of γ-globin for the potential treatment of β-hemoglobinopathy. A&C-BEmax is a valuable tool not only for dissecting the function of genomic sequence at a single base resolution but also for the therapeutics of genetic disorders. This step-by-step protocol is related to the publication “Dual base editor catalyzes both cytidine and adenine base conversions in human cells” in Nature Biotechnology.
Comparative transcriptome analysis provides insights into seed germination in herbicide resistance Polypogon fugax
Authors: Yu Xiaoyue , Tang Wei , Yang Yongjie , Zhang Jianping , Lu Yongliang 
Published: June 2020

Abstract
Background

Herbicide resistant mutations are predicted to exhibit fitness cost under herbicide-free conditions. Asia minor bluegrass (Polypogon fugax) is a common weed species in the winter crops. Our previous study established a P. fugax accession (LR) resistant to acetyl-CoA carboxylase (ACCase) inhibiting herbicides. Besides, LR also exhibited fitness cost, like lower germination, relative to the sensitive plants (LS). But little is known about the gene expression profile of seed germination process in herbicide resistance P.fugax.
Results

The maximum germination (gMAX) of LS reached 94%, while that of LR was 52%. Exogenous gibberellin and abscisic acid synthesis inhibitor fluridone could break the dormancy of LR, increasing gMAX to 78% and 94%, respectively. A comparative transcriptome was conducted to analyze the genes expression profile of LS and LR at two germination time points. A total of 11,856 and 23,123 differentially expressed genes (DEGs) were identified in LS and LR at two time points. Most of DEGs were involved in lipid metabolism, carbohydrate metabolism, amino acid metabolism and secondary metabolites biosynthesis. Four genes related to GA biosynthesis and signal transduction showed higher expression in LS and five genes related to ABA biosynthesis and signal transduction showed higher expression in LR during germination. Twenty-four genes involved in glycolysis, TCA cycle, pentose phosphate pathway and fatty acid metabolism had higher expression levels in LS than LR during germination.
Conclusions

Our results indicated that GA biosynthesis and transduction, carbohydrate and fatty acid metabolism was more actively expressed in LS plants, resulting in stronger germination ability of LS relative to LR. The highly expressed genes in ABA biosynthesis and transduction contribute to maintain the seed dormancy of LR plants. This study provided new insight into transcriptional changes and interaction in seed germination process of P. fugax, and compared the differential expression profile between the herbicides resistant and sensitive plants during seed germination.
Proteomic analysis of differentially expressed proteins in endothelial cells under low shear stress
Authors: Ren Peile , Zhao Yiwei , Zhang Daifang , Shinge Shafiu Adam U , Yu Fengxu , Nie Yongmei 
Published: June 2020

Abstract
Background: Shear stress (SS) affects the morphology, proliferation, differentiation and migration of endothelial cells, and regulates protein expression.Methods: This study aims to conduct a proteomic analysis of human umbilical vein endothelial cells (HUVECs) under low SS. HUVECs were cultured on glass slides (test group n=30; control group n=30) and transferred to parallel-plate flow chamber. The test group was subjected to low SS at 4.58 dyne/cm2 for 2 hours, and the control group was maintained under static conditions. Approximately 2´107 cells were collected from each group and analyzed by two-dimensional electrophoresis. The protein from each spot were identified by mass spectrometry. The biological functions and processes associated with the identified proteins were evaluated using the NCBInr, SwissProt, and DAVID databases. The protein expression of 14-3-3 proteins, annexin I, annexin II, zyxin, filamin A, filamin B, Ras-related nuclear protein (Ran), and talin were assessed by Western blotting.Results: A total of 234 proteins were analyzed; 14 proteins were upregulated and 78 were downregulated in the test group compared with the control group. The expression of 14-3-3 proteins, annexin I, annexin II, zyxin, filamin A, and filamin B was significantly decreased, whereas the expression of Ran and talin was significantly increased in the test group.Conclusion: The current results provide evidence that SS changes the protein profile of HUVECs.
Novel Surveillance of Occupational Injury Powered by Machine Learning Using Chief Complaint at Emergency Triage
Authors: Weng Ting-Chia , Wei Yu-Ting , Chan Tsung-Yu , Chen Wen-Chau , Chen Ming-Hong , Wang Jung-Der , Li Chung-I , Kuo Yau-Chang 
Published: June 2020

Abstract
Background: Underreporting of occupational injuries remains a global health issue, and warrants social awareness. To assist surveillance of occupational injuries, we developed an automatic screening model using chief complaint at emergency department to improve detection and reporting of occupational injuries.Methods: A total of 181,843 emergency visits aged 15 to 65 year-old were included at a medical center in Taiwan from April, 2015 to March, 2018. The retrospective cohort composed of 9,307 cases of occupational injuries and 172,536 controls. We applied the first 30 months as training dataset, and the following 6 months for prospective testing. Natural Language Processing (NLP) was applied to analyze patients’ chief complaints. The sentences were processed by JiebaR, a Chinese text segmentation technique, reviewed by two occupational physicians, and transformed by a word-embedding model using Glove. Logistic regression was conducted to predict suspected cases of occupational injuries.Results: The prediction model using the chief complaint alone can achieve an overall AUC of 0.936 [95% confidence interval (CI): 0.931 to 0.941], with high sensitivity (93.1%, 95% CI: 91.8 to 94.4) and adequate specificity (84.8%, 95% CI: 84.4 to 85.2). Patients in the most urgent or severe conditions showed the highest accuracy around 90%. We also observed increasing trends of referral to specialists of occupational medicine, and reimbursement rate.Conclusions: Interdepartmental coordination and integration of machine learning may augment the detection of occupational injuries at the emergency triage and improve the reporting, compensation, and prevention of occupational injuries.Contributions to the literaturel This novel surveillance powered by supervised machine learning can help to identify occupational injuries at the emergency triage, and it achieves 93.1% sensitivity and 84.8% specificity.l Along with the system implementation, there are trends of increasing reimbursement rate in the National Labor Insurance and increasing case referrals to specialists of occupational medicine.l Combining the use of Artificial Intelligence (AI) with conventional medical information, such as chief complaints, may provide an effective surveillance system for occupational injuries at hospitals. l Effective screening of labors who suffer from occupational injuries will promote Total Worker Health.
Treatment Pattern and Overall Survival of Patients with Advanced Non-Squamous Non-Small Cell Lung Cancer: A Multicentric Real-World Study in China (CTONG1506)
Authors: Zhou Qing , Song Yong , Zhang Xin , Chen Gongyan , Zhang Yiping , Yu Ping , Chen Jianhua , Yu Zhuang , Hu Yi , Song Xia , Zhong Diansheng , Feng Guosheng , Yang Lulu , Zhan Lu jing , Ma Xiao , Lu Xingwei , Wu Yi-Long 
Published: June 2020

Abstract
Background:CTONG1506, an observational study assessed the real-world treatment patterns and overall survival (OS) of Chinese advanced non-squamous non-small cell lung cancer (NSCLC) patients in current treatment practices.Methods:Patients initiated with 1st line therapy were identified from 12 tertiary hospitals across China. Survival data were collected 1- and 2-years after study initiation. OS was estimated using the Kaplan-Meier method.Results:Among 540 patients with survival data, median OS was 21.4 months (95% CI: 18.1–25.5), and 2-year OS rate was 46.3% (95% CI: 42.0%-51.0%). Median OS for patients with epidermal growth factor receptor (EGFR+) mutation (n = 203), anaplastic lymphoma kinase (ALK+) rearrangement (n = 24), EGFR-/ALK- were 27.9 months (95% CI: 23.4-NA), 24.5 months (95% CI: 18.1-NA), and 15.7 (95% CI: 13.1–21.1) months, respectively. Median OS was not reached in EGFR exon 19 deletion patients compared to EGFR exon 21 L858R mutation patients (21.4 months, 95% CI: 16.7–35.6, P = 0.038). 93 EGFR + patients received tyrosine kinase inhibitor (TKI) alone, 21 received chemotherapy alone and 66 received TKI and chemotherapy [median OS 25.5 months, 18.1 months, and 35.6 months, respectively]. For EGFR + patients, TKI alone was the preferred therapy in 1st (58.2%) and 2nd (56.8%) line when compared to chemotherapy (35.1% and 31.1% respectively). In 3rd line, chemotherapy was preferred (46.0%) over TKI only (38.0%) in these patients.ConclusionsOS for patients with advanced non-squamous NSCLC patients aligned with previous trials. EGFR + patients who received both TKI and chemotherapy had longer median OS, which is consistent with results from other trials.
WITHDRAWN: Long-term Elevated Siglec-10 in Cerebral Spinal Fluid Heralds Better Prognosis for Patients with Aneurysmal Subarachnoid Hemorrhage
Authors: Gao Sen , Peng Zheng , Chen Xiang-Xin , Liu Xun-Zhi , Wang Han , Lu Yue , Zhuang Zong , Hang Chun-Hua , Li Wei 
Published: June 2020

Abstract
Background
The presence of aneurysmal subarachnoid hemorrhage (aSAH) is usually accompanied by excessive inflammatory response leading to the destruction of central nervous system, while the sialic acid-binding Ig-like lectin 10 (Siglec-10) is a factor recognized as having the ability to modify the inflammatory microenvironment, indicating a potential protective effect in aSAH.
Methods
We collected the cerebrospinal fluid (CSF) of control group and aSAH patients at different time points, measured the Siglec-10 concentration by the enzyme-linked immunosorbent assay (ELISA), and evaluated the changes of Glasgow Outcome Scale (GOS) and Glasgow Coma Scale (GCS) in the disease progression.
Results
Our data showed that the Siglec-10 level in CSF could respond to aSAH and quickly rose to a peak, then fell back to a stable range slightly higher than the normal range. Severely impaired patients were likely to maintain high levels of Siglec-10 for longer periods. Generally, having high-level and long-term Siglec-10 indicated a better prognosis.
Conclusions
These results suggested that Siglec-10 could possibly reduce the degree of inflammatory response related to the damage-associated molecular patterns (DAMPs) by regulating the balance between pro-inflammatory and anti-inflammatory, thereby improving the patient's prognosis. These findings might provide new treatment perspectives for aSAH and other inflammation-related diseases.
A simple kinetic modelling of coronavirus infection in a system
Authors: NaaderAlizadeh  
Published: June 2020

Abstract
In this paper, provide an analysis of data on the characteristics of patients with confirmed COVID-19 infection throughout Iran and other countries such as China, S. Korea,USA, Italy, Spain, Germany, France, UK, Austria, and Argentina, and presented a kinetic modelling for prediction of infection, death, recovery and patients populations.The predict results of infection, death and recovery rate constants helps to interpret of public sentiment on disseminating related health information, and comparison different governments behavior to overcoming the outbreak of the coronavirus (COVID-19). Calculation results are in agreement with the census data of the coronavirus infection in many regions of the world.