bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fuziline alleviates isoproterenol‐induced myocardial injury by inhibiting ROS‐triggered endoplasmic reticulum stress via PERK/eIF2α/ATF4/Chop pathway
Authors: Fan Cai‐lian , Yao Zhi‐hong , Ye Meng‐nan , Fu Lei‐lei , Zhu Guo‐nian , Dai Yi , Yao Xin‐sheng 
Published: December 2019

AbstractFuziline, an aminoalcohol‐diterpenoid alkaloid derived fromAconiti lateralisradix preparata, has been reported to have a cardioprotective activity in vitro. However, the potential mechanism of fuziline on myocardial protection remains unknown. In this study, we aimed to explore the efficacy and mechanism of fuziline on isoproterenol (ISO)‐induced myocardial injury in vitro and in vivo. As a result, fuziline effectively increased cell viability and alleviated ISO‐induced apoptosis. Meanwhile, fuziline significantly decreased the production of ROS, maintained mitochondrial membrane potential (MMP) and blocked the release of cytochrome C, suggesting that fuziline could play the cardioprotective role through restoring the mitochondrial function. Fuziline also could suppress ISO‐induced endoplasmic reticulum (ER) stress via the PERK/eIF2α/ATF4/Chop pathway. In addition, using ROS scavenger NAC could decrease ISO‐induced apoptosis and block ISO‐induced ER stress, while PERK inhibitor GSK2606414 did not reduce the production of ROS, indicating that excess production of ROS induced by ISO triggered ER stress. And fuziline protected against ISO‐induced myocardial injury by inhibiting ROS‐triggered ER stress. Furthermore, fuziline effectively improved cardiac function on ISO‐induced myocardial injury in rats. Western blot analysis also showed that fuziline reduced ER stress‐induced apoptosis in vivo. Above these results demonstrated that fuziline could reduce ISO‐induced myocardial injury in vitro and in vivo by inhibiting ROS‐triggered ER stress via the PERK/eIF2α/ATF4/Chop pathway.
Coronally advanced flap and composite restoration of the enamel with or without connective tissue graft for the treatment of single maxillary gingival recession with non‐carious cervical lesion. A randomized controlled clinical trial
Authors: Cairo Francesco , Cortellini Pierpaolo , Nieri Michele , Pilloni Andrea , Barbato Luigi , Pagavino Gabriella , Tonetti Maurizio 
Published: December 2019

AbstractBackgroundAim of this study was to compare coronally advanced flap (CAF) and composite restoration of the cement–enamel junction (CEJ) with or without connective tissue graft (CTG) for treatment of single maxillary gingival recession with non‐carious cervical lesion (NCCL).Material and MethodsThirty patients with single gingival recessions and previously restored NCCL were randomly allocated to the two groups. A masked examiner evaluated recession reduction (RecRed), complete root coverage (CRC), keratinized tissue (KT) gain, increase in gingival thickness (GT), patient satisfaction and Root coverage Esthetic Score (RES).ResultsNo significant difference for RecRed and CRC was detected at 12 months. CAF + CTG resulted in greater increase of KT width and thickness (p < .001). An interaction between baseline GT and type of treatment was reported, suggesting that when baseline GT was ≤0.84 mm adding CTG led to higher RecRed, while for values >0.84 mm the use of CAF was associated with better outcomes. Similarly, CAF alone provided better final RES score for baseline GT > 0.82 mm.ConclusionBoth procedures were effective for root coverage at single RT1 recession with previously restored CEJ. Adding a CTG under CAF should be considered for Rec with thin gingival phenotype.
Morphology and Phylogeny of Two Novel Pleurostomatids (Ciliophora, Litostomatea), Establishing a New Genus
Authors: Pan Hongbo , Zhang Qianqian , Dong Jingyi , Jiang Jiamei 
Published: December 2019

AbstractPleurostomatida Schewiakoff, 1896 is a cosmopolitan order of ciliates. In the present study, we investigated two new pleurostomatid species, Apolitonotus lynni gen. et sp. nov. and Protolitonotus clampi sp. nov., with state‐of‐the‐art methods. Apolitonotus lynni lacks its oral extrusomes and its right kineties form an anterior semi‐suture near the dorsal margin. Based on these two features, the new genus Apolitonotus was established within the Protolitonotidae Wu et al., 2017. Protolitonotus clampi differs from its congeners by its size of 80–130 × 15–30 μm, 4–6 left, and 9–11 right kineties, extrusomes arranged along the oral slit, and two macronuclear nodules. Because Litonotus antarcticus possesses an anterior semi‐suture and oral extrusomes, it was transferred to the genus Protolitonotus, becoming P. antarctius comb. nov. (basionym Litonotus antarcticus Song and Wilbert, 2002). Phylogenetic analyses based on SSU rDNA sequences suggest a sister group relationship of P. clampi and the family Kentrophyllidae, and A. lynni is adelphotaxon to Litonotus gracilis, both within the order Pleurostomatida. Based on the new findings, an improved diagnosis for Protolitonotus was also provided.
Characteristics and composition of emulsion‐based functional low‐fat chicken meat balls fortified with dietary fiber sources
Authors: Santhi Dhanasekar , Kalaikannan Athiappan , Natarajan Amirthalingam 
Published: December 2019

AbstractThis study was conducted with the objective of fortifying emulsion‐based low‐fat chicken meat balls with dietary fiber sources and studying their characteristics and composition. Three experiments were conducted in which chicken meat balls were performed with the addition of pearl millet flour (PMF) and wheat flour (WF) combination in Experiment 1, with grape pomace powder (GPP) and pomegranate pomace powder (PPP) combination in Experiment 2, and with carrot pomace powder (CPP) and beetroot pomace powder (BPP) combination in Experiment 3 along with a control in each experiment and assessed for physicochemical and sensory characteristics. One best treatment from each experiment was selected based on the physicochemical and sensory characteristics, and the proximate composition of those treatments was assessed. Ether extract level was significantly (p ≤ .01) lower in the CPP–BPP treatment. Total ash was significantly (p ≤ .01) higher in the PMF–WF treatment. Insoluble fiber level was significantly (p ≤ .01) higher in GPP–PPP and CPP–BPP treatments. Inclusion of the fruit and vegetable sources had improved the fiber level, and inclusion of millets had enhanced the total ash content of the chicken meat balls.Practical applicationsMeat is protein‐rich food with high biological value containing various vitamins, minerals, and polyunsaturated fatty acids. Since meat is devoid of dietary fiber, which is considered as its major disadvantage, adding dietary fiber sources to meat products would enhance the nutritional value of meat. At present, chicken meat is mostly preferred by the consumers due to its leanness and low cost, and hence developing a functional chicken meat product would be an apt choice. Utilization of the common sources that are used in the regular cuisine/diet would satisfy the consumers to accept the product without any hesitation. In this study, common food ingredients such as pearl millet, wheat, grape, pomegranate, carrot, and beetroot had been used as functional components to fortify the chicken meat balls.
Microbial safety and probiotic potential of packaged yogurt products in Pakistan
Authors: Aziz Ghazal , Zaidi Arsalan , Bakht Urooj , Parveen Naila , Ahmed Ibrar , Haider Zeeshan , Muhammad Tariq 
Published: December 2019

AbstractYogurt is a health food with notable market production and demand. Because of this, we conducted a study on prominent commercial brands of yogurts in Pakistan for microbial content and the probiotic potential of the contained lactic acid bacteria (LAB), in the context of their label claims. All contained viable LAB, but the numbers (cfu g−1) varied considerably. Three of the products made explicit probiotic claims, but LAB from these displayed no probiotic attributes per WHO‐FAO guidelines. The yogurt starter and nonstarter Lactobacillus strains had no gelatinase or hemolytic activity and exhibited significant antibacterial activity against some human pathogens. One brand with a probiotic claim contained an L. acidophilus strain that showed cholesterol assimilation activity in vitro. Some potential human pathogens that were hemolytic and resistance to β‐lactam antibiotics were also detected in the products. The findings demonstrate a need for better quality control and regulation to ensure safety and efficacy of yogurt products.
Combined treatment with the histone deacetylase inhibitor LBH589 and a splice‐switch antisense oligonucleotide enhances SMN2 splicing and SMN expression in Spinal Muscular Atrophy cells
Authors: Pagliarini Vittoria , Guerra Marika , Di Rosa Valentina , Compagnucci Claudia , Sette Claudio 
Published: December 2019

AbstractSpinal muscular atrophy (SMA) is a motor neuron disease caused by loss of function mutations in the Survival Motor Neuron 1 (SMN1) gene and reduced expression of the SMN protein, leading to spinal motor neuron death, muscle weakness and atrophy. Although humans harbour the highly homologous SMN2 gene, its defective splicing regulation yields a truncated and unstable SMN protein. The first therapy for SMA was recently approved by the Food and Drug Administration and consists of an antisense oligonucleotide (Nusinersen) rendering SMN2 functional and thus improving patients’ motor activity and quality of life. Nevertheless, not all patients equally respond to this therapy and the long‐term tolerability and safety of Nusinersen are still unknown. Herein, in vivo splicing assays indicated that the HDAC inhibitor LBH589 is particularly efficient in rescuing the SMN2 splicing defect in SMA fibroblasts and SMA type‐I mice‐derived neural stem cells. Western blot analyses showed that LBH589 also causes a significant increase in SMN protein expression in SMA cells. Moreover chromatin immunoprecipitation analyses revealed that LBH589 treatment induces widespread H4 acetylation of the entire SMN2 locus and selectively favors the inclusion of the disease‐linked exon 7 in SMN2 mature mRNA. The combined treatment of SMA cells with sub‐optimal doses of LBH589 and of an antisense oligonucleotide that mimic Nusinersen (ASO_ISSN1) elicits additive effects on SMN2 splicing and SMN protein expression. These findings suggest that HDAC inhibitors can potentiate the activity of Nusinersen and support the notion that ‘SMN‐plus’ combinatorial therapeutic approaches might represent an enhanced opportunity in the scenario of SMA therapy.
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Lacunar syndromes: Are they all equally benign?
Authors: Narasimhalu Kaavya , Woon Fung Peng , Ng Szu Chyi , De Silva Deidre Anne 
Published: December 2019

AbstractBackgroundThere is a paucity of prognostic data following clinical lacunar syndromes, particularly comparisons between the various lacunar syndromes.AimsTo compare the outcomes, disability at discharge, at 1 and 3 months, use of rehabilitation facilities and duration to return to work/daily activities, between patients with various clinical lacunar syndromes.MethodsIschemic stroke patients with clinical lacunar syndromes were prospectively recruited. Functional outcomes were measured by the modified Rankin Scale (mRS), with unfavorable outcome defined as mRS > 1. Use of rehabilitation facilities and duration to return to work/ daily activities was also ascertained. Logistic regression analyses were performed to determine predictors of outcome measures.ResultsOf the 192 ischemic stroke patients (mean age 62 ± 11 years) with clinical lacunar syndromes, 72(37%) patients had ataxic hemiparesis, 53(28%) patients had pure motor syndrome, 41(21%) had sensorimotor syndrome, 17(9%) had pure sensory syndrome, and 9(5%) had clumsy hand dysarthria syndrome. Compared to pure sensory strokes, patients with pure motor syndrome (OR 7.72 CI 1.5‐38), sensorimotor syndrome (OR 6.06, CI 1.18‐31), and ataxic hemiparesis (OR 18.1 CI 3.79‐91) were more likely to have unfavorable outcome at 1 month, but there was no difference at 3 months. There was no independent association between specific lacunar syndromes with use of rehabilitation facilities and duration to return to work/ daily activities.ConclusionsNearly 1/3 of patients with clinical lacunar syndromes have unfavorable 3‐month functional outcome. There were differences in the functional status at discharge between clinical lacunar syndromes, but not for other outcomes.
Sjögren syndrome combined with myositis which is pathologically compatible with inclusion body myositis
Authors: Mitsutake Akihiko , Arakawa Akira , Sato Tatsuya , Katsumata Junko , Seki Tomonari , Maekawa Risa , Hideyama Takuto , Nishino Ichizo , Shiio Yasushi 
Published: December 2019

AbstractA 60‐year‐old woman previously diagnosed as Sjögren syndrome (SjS) presented with a three‐year history of progressive muscle weakness in the neck and predominantly proximal part of the four limbs. Pathological study showed patchy, dot‐like p62 aggregates in muscle fibers in combination with increased HLA‐ABC and ‐DR expression, which is pathologically consistent with sporadic inclusion body myositis (sIBM). Oral prednisolone improved muscle strength. Three years after the initiation of treatment, the patient still can walk. Myositis in our patient, albeit pathologically characterized by features of sIBM, responded well to prednisolone.
Tumor genetic heterogeneity analysis of chronic sun‐damaged melanoma
Authors: Sanna Adriana , Harbst Katja , Johansson Iva , Christensen Gustav , Lauss Martin , Mitra Shamik , Rosengren Frida , Häkkinen Jari , Vallon‐Christersson Johan , Olsson Håkan , Ingvar Åsa , Isaksson Karolin , Ingvar Christian , Nielsen Kari , Jönsson Göran 
Published: December 2019

AbstractChronic sun‐damaged (CSD) melanoma represents 10%–20% of cutaneous melanomas and is characterized by infrequent BRAF V600E mutations and high mutational load. However, the order of genetic events or the extent of intra‐tumor heterogeneity (ITH) in CSDhigh melanoma is still unknown. Ultra‐deep targeted sequencing of 40 cancer‐associated genes was performed in 72 in situ or invasive CMM, including 23 CSDhigh cases. In addition, we performed whole exome and RNA sequencing on multiple regions of primary tumor and multiple in‐transit metastases from one CSDhigh melanoma patient. We found no significant difference in mutation frequency in melanoma‐related genes or in mutational load between in situ and invasive CSDhigh lesions, while this difference was observed in CSDlow lesions. In addition, increased frequency of BRAF V600K, NF1, and TP53 mutations (p < .01, Fisher's exact test) was found in CSDhigh melanomas. Sequencing of multiple specimens from one CSDhigh patient revealed strikingly limited ITH with >95% shared mutations. Our results provide evidence that CSDhigh and CSDlow melanomas are distinct molecular entities that progress via different genetic routes.
Clinical course of porokeratosis ptychotropica over 7 years in an otherwise healthy child
Authors: Ho Tina , Schwentker Ann R. , Barron David R. , Lucky Anne W. 
Published: December 2019

AbstractPorokeratosis ptychotropica is an unusual variant of porokeratosis characterized by papules and plaques located on the buttocks and gluteal cleft and showing multiple coronoid lamellae on histology. In this case report, we present the longitudinal clinical course of porokeratosis ptychotropica in a pediatric patient with individual red‐brown hyperkeratotic lesions that enlarged and became confluent prior to surgical intervention. We also discuss the etiology of porokeratosis ptychotropica and review current as well as future treatment options for the disease.
The impact of depressive mood and cognitive impairment on quality of life of the elderly
Authors: Voros Viktor , Martin Gutierrez David , Alvarez Federico , Boda‐Jorg Adrienn , Kovacs Attila , Tenyi Tamas , Fekete Sandor , Osvath Peter 
Published: December 2019

BackgroundThe quality of life (QOL) of the elderly can be influenced by numerous factors. We assessed QOL, cognitive functions, depression and clinical data in elderly aged 65 and over with the aim of analysing factors affecting their QOL.MethodsSemi‐structured interviews were conducted with elderly over the age of 65, and their QOL, cognitive functions and depressive symptoms were assessed by validated clinical tests and screening tools.ResultsThe correlation between QOL scales and cognitive tests was not significant. In contrast, the results of depression scales showed significant negative correlation with the scores of the QOL scales. A better QOL was determined by lower age, lack of depressive symptoms, and higher scores in the QOL‐AD (Alzheimer's disease) scale. Depressive mood has much more negative impact on the QOL of the elderly than cognitive impairment.ConclusionsOur results demonstrated a close correlation between QOL and depressive mood in the elderly. The early detection and effective management of affective and cognitive symptoms in the elderly can not only restore mental health but may also improve their QOL.
Efficiency‐adjusted public capital, capital grants, and growth
Authors: Crivelli Ernesto 
Published: December 2019

AbstractA debate on whether capital grants, and especially European Union (EU) funds, actually contribute to growth has gained prominence lately. This article empirically assesses the relationship between the quality of public investment, capital grants, and growth in a sample of 43 emerging and peripheral economies over 1991–2015. To this end, the contribution of public capital to growth is estimated using efficiency‐adjusted public capital stock series, which reflects the quality of public investment management institutions. In addition, the determinants of effective public investment are analyzed. The results suggest that capital grants contribute positively to effective public investment, and the latter is significant in explaining variations in economic growth. Finally, the article illustrates the impact of raising EU funds absorption on potential growth in emerging and peripheral EU countries.
Radiogenic trigger and driver for continental rifting and initial ocean spreading
Authors: Maystrenko Yuriy Petrovich , Slagstad Trond 
Published: December 2019

AbstractClosure and opening of oceans on time‐scales of a few hundred million years is a fundamental tectonic process on Earth, typically referred to as a “Wilson cycle”. Subduction of oceanic and continental crust leading up to and during continent–continent collision can refertilize and enrich the orogenic continental lithospheric mantle in heat‐producing elements. The resulting thermal anomaly weakens the lithosphere and, along with structural weaknesses (e.g. sutures), make this orogenic lithosphere more prone to rifting given an extensional stress field. Thermal modelling shows that anomalously hot lithosphere can focus asthenospheric upwellings over time‐scales of a few hundred million years. Processes related to closure of oceans thus provide a mechanism for later localization of rifting and an extensional driving force.
Detrital zircon geochronology analysis of the Late Mesozoic deposition in the Turpan‐Hami basin: Implications for the uplift history of the Eastern Tian Shan, north‐western China
Authors: Shen Tianyi , Chen Yue , Wang Guocan , Ji Junliang , Wang Yong , Zhang Pan , Sotiriou Paul , Guo Rong , Xu Zezhuang 
Published: December 2019

AbstractAnalysing the provenance changes of synorogenic sediments in the Turpan‐Hami basin by detrital zircon geochronology is an efficient tool to examine the uplift and erosion history of the easternmost Tian Shan. We present detrital zircon U‐Pb analysis from nine samples that were collected within marginal lacustrine Middle‐Late Jurassic and aeolian‐fluvial Early Cretaceous strata in the basin. Middle‐Early Jurassic (159–172 Ma) zircons deriving from the southern Junggar dominated the Middle Jurassic sample from the western Turpan‐Hami basin, whereas Permian‐Carboniferous (270–330 Ma) zircons from the Bogda mountains were dominant in the Late Jurassic to Early Cretaceous samples. Devonian‐Silurian (400–420 Ma) and Triassic (235–259 Ma) zircons from the Jueluotage and Harlik mountains constituted the subordinate age groups in the Late Jurassic and Early Cretaceous samples from the eastern basin respectively. These provenance transitions provide evidence for uplift of the Bogda mountains in the Late Jurassic and the Harlik mountains since the Early Cretaceous.
Effect of nurse‐led care on quality of care and level of HbA1C in patients with diabetic foot ulcer: A randomized clinical trial
Authors: Dehghan Nayeri Nahid , Samadi Nasrin , Larijani Bagher , Sayadi Leila 
Published: December 2019

AbstractDiabetic foot ulcer (DFU) is a serious and costly complication in diabetes which affects different aspects of life and can reduce patient's quality of life. Various views to manage DFU have been introduced. The nurse‐led team, as a multidisciplinary team, can be effective due to a holistic approach to some disease management; but in patients with DFU it has not been assessed. Therefore, this study was designed to determine the effect of nurse‐led care on quality of care (QOC) and improvement of HbA1C in Patients with DFU. This was a randomized clinical trial study performed on 52 patients with DFU. Patients were randomly assigned into two groups: nurse‐led care intervention and standard care. The study was conducted in one of the hospitals affiliated to Tehran University of Medical Sciences. Data were collected using two questionnaires: Demographic characteristics and Quality Patient Care Scale (QUALPACS) and taking a blood sample for HbA1C. The nurse‐led Care interventions were conducted in three stages: Integrated, Interdisciplinary, and Comprehensive. Descriptive and analytic statistical methods were used to analyze the data. P < .05 was considered significant. The results demonstrated that according to repeated measures test, (before, 4 and 12 weeks after the intervention), the level of QOC dimensions (Psychosocial, communication and physical aspect) in the nurse‐led group had a significant difference with control group (P < .0001). Also, according to the results of Independent t test, there was a significant difference in total QOC scoring and HbA1c between intervention and control groups after the intervention and follow up (P < .0001). Considering the burden of diabetes and DFUs, it seems that the establishment of a nurse‐led care approach can be an effective strategy to manage and treat these patients, and eliminate the disruption of care and achieve optimal care quality.