bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Association Between TP53 rs1625895 Polymorphism and the Risk of Sarcopenic Obesity in Iranian Older Adults: A Case-Control Study
Authors: Montazeri-Najababady Nima , Dabbaghmanesh Mohammad Hossein , Nasimi Nasrin , Sohrabi Zahra , Chatrabnous Nazanin 
Published: July 2020

Abstract
Background: Aging and obesity are the two major global health concerns. Sarcopenia, an age-linked disease, wherein a progressive loss of muscle volume, muscle strength, and physical activity occurs. In this study we evaluated the association of TP53 rs1625895 polymorphism with the susceptibility to sarcopenic obesity in Iranian old-age subjects. Total of 206 old individuals (45 sarcopenic and 161 non-sarcopenic) were recruited in this research and genotyped by PCR–RFLP. BMI, Skeletal Muscle Mass Index (SMI), body composition, Handgrip Strength (HGS), Gait Speed (GS), and biochemical parameters were measured. Chi-square test was done for genotypes and alleles frequency. Linear regression was applied to find the correlation between TP53 rs1625895 polymorphism, and biochemical and anthropometric parameters. The correlation between TP53 rs1625895 and the risk of sarcopenia and sarcopenic obesity was investigated by logistic regression.Results: G allele was significantly higher in sarcopenic obesity group [P =0.037, OR (CI 95%)=1.9 (1.03-3.5)] compared to A allele. BMI (P= 0.049) and LDL (P=0.04) were significantly differed between genotypes when GG was compared to AA/AG genotype. The results revealed when GG genotype compared to AA/AG genotype in adjusted model for age, the risk of sarcopenic obesity [P value= 0.011, OR (CI 95%); 2.72 (1.25-5.91)] increased. Similarly, GG/AG genotype increased the risk of sarcopenic obesity [P value= 0.028, OR (CI 95%); 2.43 (1.10-5.36)] in adjusted model for age compared to AA genotype.Conclusion: We concluded that TP53 rs1625895 polymorphism may increase the risk of sarcopenic obesity in Iranian population.
Development and Evaluation of a Novel Music-based Therapeutic Device for Upper Extremity Movement Training
Authors: Schaffert Nina , Janzen Thenille Braun , Ploigt Roy , Schlüter Sebastian , Vuong Veronica , Thaut Michael H. 
Published: July 2020

Abstract
Background

Restoration of upper limb motor function and patient functional independence are crucial treatment targets for neurologic recovery. Growing evidence indicates that music-based intervention is a promising therapeutic approach for the restoration of upper extremity functional abilities in neurologic conditions. In this context, music technology may be particularly useful to increase the availability and accessibility of music-based therapy and assist therapists in the implementation and assessment of targeted therapeutic goals. In the present study, we describe and evaluate a novel music-based therapeutic device (SONATA) for upper limb extremity movement training.
Methods

The device consists of a graphical user interface generated by a single-board computer displayed on a 32” touchscreen with build-in sound speakers controlled wirelessly by a computer tablet. The system includes two operational modes that allow users to play musical melodies on a virtual keyboard or draw figures/shapes whereby every action input results in controllable sensory feedback. Four functional tests were performed with 21 healthy individuals (12 males, age 26.4 ± 3.5 years) to evaluate the device’s operational modes and main features, such as presenting sequences of audiovisual stimuli at a pre-defined order (Tasks 1–3), displaying different shapes (Task 4), and collecting response and movement data (e.g., reaction time, correct/incorrect responses, and timing data).
Results

The results indicate feasibility and ease of use of the device, as shown by the participants’ performance accuracy in all tasks. The findings also demonstrate the reliability of the data acquired automatically by the system as we replicated the results of previous research showing a decrease in reaction time in sequences repeatedly presented in relation to random sequences, and that sequence length, rate and complexity affect accuracy of newly learned action sequences.
Conclusions

This device is a feasible tool for upper limb extremity movement training and opens new avenues for the the systematic evaluation of the benefits of music technologies in clinical research.
Computed Tomography Imaging Features of Malignant “Triton” Tumor for Its Clinical Diagnosis: Report of Two Cases
Authors: Li Yuan , Zeng Chun , Jiang Ning , Molloy David P. , Peng Qiling , Zhang Cheng , Shi Haoming , Chen Dan , Wu Qingchen 
Published: July 2020

Abstract
Background: malignant triton tumors (MTTs) are an extremely rare subtype of malignant periphery nerve sheave tumors (MPNSTs). Clinical diagnosis of MTTs is difficult before surgery due to its low incidence and the lack of knowledge. Therefore, to describe and summarize the computed tomography (CT) imaging characteristics of malignant triton tumors (MTTs) is of great assistance for early and preoperative diagnosis.Case presentation: Two case reports were closely observed in our hospital, with the presumptive diagnosis of MTT by CT scan examination before surgery. The diagnosis of MTT was eventually confirmed by immunochemical (IHC) assay, which verified speculation of CT scans. Huge, irregular, well-circumscribed lobulated mass-like shadows can be observed from these patients by CT scans. Besides, heterogeneity of density within the body of tumor was well-established by CT scans, together with linear septum. Meanwhile, CT scans demonstrated that calcifications were remarkable at the margin of tumor body. Conclusions: Some CT image features from two cases admitted to our hospital were presented as a reference for the preoperative diagnosis of MTTs: (i) enormity of mass-like shadow; (ii) presence of well-circumscribed lobulated shape; (iii) septum within the well-defined mass accompanied with hemorrhage, necrosis and cystic changes as well as calcification, especially within neurofibromatosis type 1 (NF-1) patients.
Catheter Care Bundle and Low Catheter Infection Rates in a Home Parenteral Nutrition Population: A 4 Year Observational Study
Authors: DeLegge Mark , Hamilton Karen Sexton , Leone Melissa , Checkley L. Allyson 
Published: July 2020

Abstract
Background
Home Parenteral Nutrition (HPN) is often a life-saving therapy for patients. One of the most common complications for HPN is catheter-related blood stream infections (CRBSI). In the home setting there is no single defined “care bundle” for the on-going maintenance of central venous catheters (CVC) for the prevention of CRBSI. We evaluated the impact of a standardized catheter care bundle in patients receiving HPN on the incidence of CRBSI.
Methods
Data collection included use of standardized tools and processes to capture patient demographics, catheter complications including CRBSI and some associated risk factors. Reported data was collected and analyzed annually and compared year-to-year from the years 2014–2017 from one national home infusion company. CRBSI reported as number of infections/1000 catheter days
Results
The CRBSI rate/1000 days was reported as 0.43, 0.31, 0.30 and 0.23 (2014, 2015, 2016, 2017 respectively). The type of catheter, number of catheter lumens and type of nursing care provided had importance. In general, single lumen central venous catheters had numerically less CRBSI than double lumen central venous catheters; peripherally-inserted central venous catheters were the most common catheter used but also had the highest percentage of CRBSI.
Conclusion
The use of a catheter care bundle in an HPN population resulted in a 4- year reported outcomes of low and continuously declining CRBSI in a large, diverse United States-based HPN population.
LncRNA ZFPM2-AS1 Promotes Metastasis and Epithelial-mesenchymal Transition of Hepatocellular Carcinoma via Targeting miR-3612/ADAM15 Axis
Authors: Yang Nan , Chen Tianxiang , Yao Bowen , Wang Liang , Liu Runkun , Niu Yongshen , Tu Kangsheng , Liu Zhikui 
Published: July 2020

Abstract
Background: Long non-coding RNAs (lncRNAs) have obtained growing attention due to their potential effects as novel regulators in various tumors. This study aimed to investigate the expression and roles of lncRNA ZFPM2-AS1 in the progression of hepatocellular carcinoma (HCC). Methods: Transwell was used to determine migration and invasion of HCC cells in vitro. The lung metastasis mouse model was established to detect tumor metastasis of HCC in vivo. The direct binding of miR-3612 to 3'UTR of DAM15 was confirmed by luciferase reporter assay. The expression of ZFPM2-AS1 and miR-3612 in HCC specimens and cell lines were detected by real-time PCR. The correlation among ZFPM2-AS1 and miR-3612 were disclosed by a dual-luciferase reporter assay, RIP assay and biotin pull-down assay.Results: In present study, we found that ZFPM2-AS1 was up-regulated in HCC tissues and cells and its upregulation was associated with TNM stage, vascular invasion, and poor prognosis of HCC patients. Functionally, gain- and loss-of-function experiments indicated that ZFPM2-AS1 promoted cell migration, invasion and EMT progress in vitro and in vivo. ZFPM2-AS1 could function as a competing endogenous RNA (ceRNA) by sponging miR-3612 in HCC cells. Mechanically, miR-3612 inhibited HCC metastasis and alternation of miR-3612 reversed the promotive effects of ZFPM2-AS1 on HCC cells. In addition, we confirmed that ADAM15 was a direct target of miR-3612 in HCC and mediated the biological effects of miR-3612 and ZFPM2-AS1 in HCC. Curcumin, an active derivative from turmeric, exerts its anticancer effects through ZFPM2-AS1/miR-3612/ADAM15 pathway. Our data identified ZFPM2-AS1 as a novel oncogenic lncRNA and correlated malignant clinical outcomes in HCC patients. Conclusions: ZFPM2-AS1 performed as oncogenic role via targeting miR-3612 and subsequently promoted ADAM15 expression in HCC. Our results revealed that ZFPM2-AS1 could be a potential prognostic biomarker and therapeutic target for HCC.
SARS-COV2 Envelope Protein (E) interacts with the Lysophosphatidic Acid Receptor 1 (LPAR1) from humans
Authors: NALLUR GIRISH 
Published: July 2020

Abstract
A proteomic screen of human proteins interacting with the SARS-COV2 Envelope (E) protein identified LPAR1 as a strong candidate. Physical association of E protein and LPAR1 was confirmed by co-immunoprecipitation and cell surface staining. LPAR1-E protein interaction was confirmed in all eight human cell lines tested. Many additional proteins participating in the E protein interactions network were also enriched from each of the cell lines, some of which were cell type specific. These findings suggest that LPAR1 is likely a cell surface receptor for the E protein, and pave the way for follow-on studies aimed at understanding the biological significance of the interactions in SARS-COV disease, including the signaling mechanisms.
Baseline predictors of attrition in a randomised controlled trial of treatments for people with recurrent binge eating episodes associated to overweight or obesity
Authors: Mannan Haider , Palavras Marly , Hay phillipa , Claudinho Angelica 
Published: July 2020

Abstract
PurposeUnderstanding the high rate of treatment attrition in trials of people with eating disorders is important as it can compromise the quality of the trials. In clinical practice, it may also contribute to illness chronicity, relapse, and costs. Thus, we investigated factors associated with treatment attrition to a new manualised psychotherapy HAPIFED compared to CBT-E, for individuals with Bulimia Nervosa or Binge Eating Disorder comorbid with overweight or obesity.MethodsIn total, 98 participants were recruited with half randomised to HAPIFED and half to the control intervention CBT-E, all administered in groups of up to 10 participants. An investigator external to the site conducted the random allocation, which was concealed from the statistician involved in the analysis, and known only to the therapists until the finalization of the 12-month follow-up after the end of active treatment. Three scenarios in the timeline treatment of a total of 30 sessions were assessed: 33% or 60% or 75% of presence. Logistic regression analysis was performed to find the correlates of attrition.ResultsNone of the six variables - frequency of binge eating episodes, purging, eating disorder symptom severity, weight, illness duration and mental health-related quality of life - significantly predicted attrition at 33%, but longer illness duration predicted lower treatment attrition at both 60% and 75% presence of the interventions. Also for 75% presence, lower weight predicted lower treatment attrition.ConclusionsLate treatment dropout was associated with lower illness length and a higher weight. More research is needed to recognize factors that may interfere with engagement in treatments aiming to avoid early dropout.Trial registrationUS National Institutes of Health clinical trial registration number NCT02464345, date of registration 1 June 2015.
Rab25 Acts as a Promising Novel Bio-Marker in the Prognosis of Patients with Hepatocellular Carcinoma
Authors: Dai Zhong , Yao Ke-Qing , Hu Xing-Sheng , Li Yi-Qun , Liu Yu-Tao , Li Shu-Luan , Ji Xue 
Published: July 2020

Abstract
Background: Rab25 was indicated to be involved in several human tumors. However, the clinical significance of Rab25 in hepatocellular carcinoma (HCC) was still unclear. The purpose of this study was to investigate the expression and prognostic value of Rab25 in HCC.Methods: The relative mRNA expression levels of Rab25 in HCC tissues and adjacent normal tissues were detected by quantitative real-time polymerase chain reaction (qRT-PCR). Chi-square test was used to analyze the relationship between Rab25 expression and clinical characteristics of patients. The prognostic value of Rab25 in HCC was estimated through Kaplan-Meier method and cox regression analysis.Results: Rab25 gene expression level was significantly higher in HCC tissues than that in normal tissues (P<0.001). Importantly, the increased Rab25 expression was closely associated with TNM stage (P=0.024), metastasis (P=0.022) and invasion classification (P=0.039). Moreover, patients with high Rab25 expression tended to have obviously shorter overall survival than those with low expression of Rab25 (log rank test, P<0.001) via Kaplan-Meier analysis. Univariate and multivariate cox regression analyses revealed that Rab25 was an independent prognostic factor of HCC.Conclusions: Rab25 is up-regulated in HCC and contributes to the progression of this tumor. What’s more, Rab25 may be a potential bio-marker for the prognosis of HCC.
Transcription Factor FOXK1 Regulates the lncRNA NEAT1 to Promote Glioma Development Through Mediating KDM3A
Authors: Liu Feng , Wu Hao , Wu Guangyong , Long Jun , Dai Jin , Wang Zhifei 
Published: July 2020

Abstract
Background: Long noncoding RNAs are widely studied in glioma. However, the role of the lncRNA NEAT1 and KDM3A in glioma has not yet been reported. We aimed to reveal the role of these two lncRNAs in the development of glioma through this study.Methods: Samples from glioma patients and normal brain tissues were collected, and the expression of NEAT1 was detected by qRT-PCR. A dual-luciferase reporter gene assay, chromatin immunoprecipitation (ChIP), RNA-binding protein immunoprecipitation (RIP), and RNA pulldown experiments were used to identify the relationship between FOXK1, NEAT1, miR-128, and KDM3A. The CCK8 assay, Transwell assay and flow cytometry were used to detect cell viability, invasion and migration ability, and the cell cycle and apoptosis, respectively. Tumor formation experiments verified the effect of NEAT1 on gliomas in vivo.Results: FOXK1 and NEAT1 were significantly overexpressed in glioma tissues and cells, and NEAT1 was significantly related to WHO classification. FOXK1 bound the NEAT1 gene promoter region in glioma cells, and interference with FOXK1 inhibited NEAT1 expression. NEAT1 inhibited miR-128 expression by binding miR-128; significantly improved cell viability and invasion and migration capabilities; increased the expression of KDM3A and activated the Wnt signaling pathway. Interference with KDM3A reversed the above results. In addition, interference with NEAT1 decreased KDM3A expression and inhibited tumor growth.Conclusion: Interference with NEAT1 promoted the expression of miR-128, thereby suppressing the expression of KDM3A and inhibiting the occurrence and development of glioma, while the expression of NEAT1 was shown to be regulated by the upstream transcription factor FOXK1.
Thyroid dysfunction as a modifiable risk factor for age-related macular degeneration: A case-control investigation
Authors: Farvardin Mohsen , mousavi Seyed esmaeil , Zare Kamran , Bazdar Somaye , Farvardin Zahra , Johari Mohammadkarim 
Published: July 2020

Abstract
The authors have requested that this preprint be removed from Research Square.
Pipeline leak detection using the multiple signal classification-like method
Authors: Li Juan , Wu Ying , Lu Changgang 
Published: July 2020

Abstract
Leakages in pipelines can cause severe hazards to industries, the environment and people. For the purpose of an accurate identification of the leakage location, a transient-based leakage detection method using multiple signal classification (MUSIC)-like is applied to this paper. The localization is achieved by a one-dimensional search of leak location along the pipe, which means it involves low computational cost. The performance of the MUSIC-Like method in the cases of a single leak and multiple leaks is discussed by comparison with three spectral-based methods. In the single-leak case, the MUSIC-like algorithm provides precise localization estimation even for a high level of noise. For the multiple-leak case, the MUSIC-like method is superior to the other three methods. It is capable of identifying all leaks where the leak-to-leak distance is less than half the shortest probing wavelength. Therefore, the MUSIC-like method has an excellent performance in leak detection and location.
Rainwater as an alternative drinking water source for the Chronic Kidney Disease Of Uncertain etiology (CKDu) prone areas: a case study for Girandurukotte, Sri Lanka
Authors: Jayasooriya V. M. , Perera V. M. M. , Muthukumaran S. 
Published: July 2020

Abstract
Chronic Kidney Disease of uncertain etiology (CKDu) is a fatal disease that causes death from kidney failure due to unknown risk factors and has already affected more than 400,000 people in the rural agricultural landscape (dry zone) of Sri Lanka. The major drinking source in Sri Lanka is groundwater and it is suspected that the pollution of groundwater sources due to agricultural means has a major impact on CKDu. The primary objective of this study is to determine whether rainwater can be used as an alternative safe drinking water source in Girandurukotte area, Sri Lanka, which is known to be an area endemic for CKDu. The physical, chemical, and biological analyses were performed to compare the water quality parameters of three water sources (groundwater, surface water, and rainwater) for Girandurukotte area. The most common storage tanks in polyethylene (PE) and ferrocement (FC) were compared to assess the influence of the material of rainwater tank on water quality. The results showed that there is a significant difference in rainwater in terms of water quality compared to groundwater and surface water. Rainwater in FC and PE tanks showed significant differences (p &lt; 0.05) for some parameters however, they were still within accepted potable drinking water standards.
Diosgenin via NMDA Receptor Exerted Anxiolytic-like Effect on Maternally Separated Mice
Authors: Boroujeni Shakiba Nasiri , Lorigooini Zahra , Boldaji Fatemeh Rahimi , Amini-Khoei Hossein 
Published: July 2020

Background and aim:
Anxiety is one of the most common psychiatric disorders that lead to the disruption
of daily life and also the quality of life. Routine medications have many side effects and cause physical
dependence and psychosocial addiction. Diosgenin is a phytosteroid found in a number of herbs. The present
study aimed to investigate the anxiolytic-like effect of diosgenin in the maternal separation model in male mice
focusing on the role of NMDA receptors.

Material and Methods:
Maternal separation (MS) paradigm was performed daily (3 h) from postnatal day
(PND) 2-14. Male mice were treated with different doses of diosgenin to find effective and sub-effective doses. In
the next step, mice were treated with an effective dose of diosgenin plus NMDA and or a sub-effective dose of
diosgenin plus ketamine (NMDA antagonist). Valid behavioral tests for the evaluation of anxiety-like behavior
were performed. Then, mice were euthanized, the hippocampus was dissected out and gene expression of NMDA
receptors (NR2a and NR2b subunits) was assessed.

Results:
MS provokes anxiety-like behaviors in the open field test (OFT) and elevated plus maze (EPM) test.
Diosgenin significantly mitigated the negative effects of MS. Co-administration of NMDA attenuated anxiolyticlike
effect of the effective dose of diosgenin, while ketamine potentiated the anxiolytic effect of sub-effective
dose of diosgenin. Furthermore, MS increased the expression of the NMDA receptor in the hippocampus which to
some extent modulated with diosgenin.

Conclusion:
Diosignin has an anxiolytic-like effect on MS mice which at least, in part, mediated through NMDA
receptors.
Cytotoxic Effects of Blue Scorpion Venom (Rhopalurus junceus) in a Glioblastoma Cell Line Model
Authors: Lozano-Trujillo Laura A. , Garzón-Perdomo Diana K. , Vargas Andrea C.R. , de los Reyes Lina M. , Avila-Rodriguez Marco F. , Gay Olivia T.G. , Turner Liliana F. 
Published: July 2020

Background:Cancer is one of the main death causes worldwide. Besides, current therapies and treatments to counter cancer, it is needed new alternatives to diminish the cell proliferation of oncogenic processes.Methods:One of the most promissory therapy includes the use of blue scorpion venom as a specific cytotoxic agent to kill tumoral cells including Glioblastoma multiforme. We show evidence of the cytotoxic effect of blue scorpion venom in a cellular model of Glioblastoma multiforme.Methods:We show evidence of the cytotoxic effect of blue scorpion venom in a cellular model of Glioblastoma multiforme.Results:Our results demonstrate that 50 μg/ml of scorpion venom is capable to diminish the viability of Glioblastoma populations.Conclusion:It is possible that the action mechanism could be associated with a loss of membrane integrity. Additionally, some metalloproteinases as MMP2 and MMP9 may also participate in the potential action mechanism.