bluuebunny/crossref_metadata_2025_split huggingface dataset:
Attracting Israeli nursing students to community nursing
Authors: Sela-Vilensky Yael , Grinberg Keren , Nissanholtz-Gannot Rachel 
Published: October 2020

AbstractBackgroundThe shift from inpatient care to community patient care has had an essential impact on the nursing profession. Despite the growing demand for community nurses in many countries, their number remains relatively low and many students do not perceive this field as an interesting career to pursue. In this review we aimed to understand if exposure of undergraduate nursing students to various nursing work settings during their studies affects their work setting choices after graduation.MethodsA literature search of papers relating to work setting preferences of nursing students in Israel and other countries was performed. Israel Ministry of Health, Nursing Administration documents and other related documents were also reviewed, with a focus on the nursing training program in Israel.FindingsWhile most first-year nursing students have limited knowledge regarding the profession, in later years, their preferences for post-graduation work settings are affected by their exposure to the various clinical fields through knowledge gained in courses together with clinical practice placements. In Israel, specific classroom courses in community nursing are allocated only 6% of the total time allocated to all classroom courses in nursing, and a single clinical placement in community nursing takes place during the third or fourth year of the nursing program, exposing students to a single aspect of community nursing during their nursing training. Studies in other countries have reported that students’ experience during clinical placement contributes to shaping students’ opinions of nurses’ roles within that field. Nursing students who had a primary healthcare placement showed greater intention for working in this setting after graduation.ConclusionsThe lack of exposure to the various aspects of community nursing during undergraduate studies contributes to a lack of motivation for entering this field. Therefore, a profound change is needed in nursing training programs’ curricula to prepare graduates to face future challenges in community nursing. Whilst both hospital and community nursing are equally important, nursing leaders and policy makers must be made aware of the various factors that contribute to new registered nurses’ preferences of hospital over community nursing and build strategies for directing nurses to work in the community in order to respond to the expected nurse shortage in this setting.
Application of intelligent orienteering based on Internet of things
Authors: Zhang Donghui , Liu Ruijie 
Published: October 2020

AbstractOrienteering has gradually changed from a professional sport to a civilian sport. Especially in recent years, orienteering has been widely popularized. Many colleges and universities in China have also set up this course. With the improvement of people’s living conditions, orienteering has really become a leisure sport in modern people’s life. The reduced difficulty of sports enables more people to participate, but it also exposes a series of problems. As the existing positioning technology is relatively backward, the progress in personnel tracking, emergency services, and other aspects is slow. To solve these problems, a new intelligent orienteering application system is developed based on the Internet of things. ZigBee network architecture is adopted in the system. ZigBee is the mainstream scheme in the current wireless sensor network technology, which has many advantages such as convenient carrying, low power consumption, and signal stability. Due to the complex communication environment in mobile signal, the collected information is processed by signal amplification and signal anti-interference technology. By adding anti-interference devices, video isolators and other devices, the signal is guaranteed to the maximum extent. In order to verify the actual effect of this system, through a number of experimental studies including the relationship between error and traffic radius and the relationship between coverage and the number of anchor nodes, the data shows that the scheme studied in this paper has a greater improvement in comprehensive performance than the traditional scheme, significantly improving the accuracy and coverage. Especially the coverage is close to 100% in the simulation experiment. This research has achieved good results and can be widely used in orienteering training and competition.
Behavioral modifications by a large-northern herbivore to mitigate warming conditions
Authors: Jennewein Jyoti S. , Hebblewhite Mark , Mahoney Peter , Gilbert Sophie , Meddens Arjan J. H. , Boelman Natalie T. , Joly Kyle , Jones Kimberly , Kellie Kalin A. , Brainerd Scott , Vierling Lee A. , Eitel Jan U. H. 
Published: October 2020

Abstract
Background
Temperatures in arctic-boreal regions are increasing rapidly and pose significant challenges to moose (Alces alces), a heat-sensitive large-bodied mammal. Moose act as ecosystem engineers, by regulating forest carbon and structure, below ground nitrogen cycling processes, and predator-prey dynamics. Previous studies showed that during hotter periods, moose displayed stronger selection for wetland habitats, taller and denser forest canopies, and minimized exposure to solar radiation. However, previous studies regarding moose behavioral thermoregulation occurred in Europe or southern moose range in North America. Understanding whether ambient temperature elicits a behavioral response in high-northern latitude moose populations in North America may be increasingly important as these arctic-boreal systems have been warming at a rate two to three times the global mean.

Methods
We assessed how Alaska moose habitat selection changed as a function of ambient temperature using a step-selection function approach to identify habitat features important for behavioral thermoregulation in summer (June–August). We used Global Positioning System telemetry locations from four populations of Alaska moose (n = 169) from 2008 to 2016. We assessed model fit using the quasi-likelihood under independence criterion and conduction a leave-one-out cross validation.

Results
Both male and female moose in all populations increasingly, and nonlinearly, selected for denser canopy cover as ambient temperature increased during summer, where initial increases in the conditional probability of selection were initially sharper then leveled out as canopy density increased above ~ 50%. However, the magnitude of selection response varied by population and sex. In two of the three populations containing both sexes, females demonstrated a stronger selection response for denser canopy at higher temperatures than males. We also observed a stronger selection response in the most southerly and northerly populations compared to populations in the west and central Alaska.

Conclusions
The impacts of climate change in arctic-boreal regions increase landscape heterogeneity through processes such as increased wildfire intensity and annual area burned, which may significantly alter the thermal environment available to an animal. Understanding habitat selection related to behavioral thermoregulation is a first step toward identifying areas capable of providing thermal relief for moose and other species impacted by climate change in arctic-boreal regions.
Development of chromosome-based T7 RNA polymerase and orthogonal T7 promoter circuit in Escherichia coli W3110 as a cell factory
Authors: Ting Wan-Wen , Tan Shih-I , Ng I-Son 
Published: October 2020

Abstract
Background
Orthogonal T7 RNA polymerase (T7RNAP) and T7 promoter is a powerful genetic element to mediate protein expression in different cells. Among all, Escherichia coli possess advantages of fast growth rate, easy for culture and comprehensive elements for genetic engineering. As E. coli W3110 owns the benefits of more heat shock proteins and higher tolerance to toxic chemicals, further execution of T7-based system in W3110 as cell factory is a conceivable strategy.

Results
Three novel W3110 strains, i.e., W3110:IL5, W3110::L5 and W3110::pI, were accomplished by chromosome-equipped T7RNAP. At first, the LacZ and T7RNAP with isopropyl-β-D-thiogalactopyranoside (IPTG) induction showed higher expression levels in W3110 derivatives than that in BL21(DE3). The plasmids with and without lacI/lacO repression were used to investigate the protein expression of super-fold green fluorescence protein (sfGFP), carbonic anhydrase (CA) for carbon dioxide uptake and lysine decarboxylase (CadA) to produce a toxic chemical cadaverine (DAP). All the proteins showed better expression in W3110::L5 and W3110::pI, respectively. As a result, the highest cadaverine production of 36.9 g/L, lysine consumption of 43.8 g/L and up to 100% yield were obtained in W3110::pI(−) with plasmid pSU-T7-CadA constitutively.

Conclusion
Effect of IPTG and lacI/lacO regulator has been investigated in three chromosome-based T7RNAP E. coli strains. The newly engineered W3110 strains possessed similar protein expression compared to commercial BL21(DE3). Furthermore, W3110::pI displays higher production of sfGFP, CA and CadA, due to it having the highest sensitivity to IPTG, thus it represents the greatest potential as a cell factory.
Antioxidant-rich Tamarindus indica L. leaf extract reduced high-fat diet-induced obesity in rat through modulation of gene expression
Authors: Kuddus Syed Abdul , Bhuiyan Mazharul Islam , Subhan Nusrat , Shohag Md Hasanuzzaman , Rahman Aura , Hossain Muhammad Maqsud , Alam Md Ashraful , Khan Ferdous 
Published: October 2020

Abstract
Background
Different parts of the medicinal plant Tamarindus indica L. are full of phytochemicals that are able to reduce elevated blood pressure, blood sugar and lipids. These pharmacological effects are due to the presence of antioxidant type compounds in those parts of the plant. This study was aimed to explore the molecular mechanism of anti-obesity effects of ethanolic extract of T. indica L. leaves (TILE) through the evaluation of biochemical parameters and gene expression analysis in high-fat diet (HFD) consuming Wistar rats.

Methods
Male Wistar rats were supplied with a standard diet (SD), or HFD, or HFD with 100 mg/kg or 200 mg/kg or 400 mg/kg TILE for 8 weeks. The body weight, liver weight, fat weight, plasma lipids, and oxidative stress-related parameters were measured. The transcript levels of different adipogenesis related transcription factors, lipogenic enzymes, and lipolytic enzymes were also evaluated by quantitative real-time PCR.

Result
Phytochemical analysis demonstrated that TILE is enriched with a substantial level of polyphenols (287.20 ± 9.21 mg GAE/g extract) and flavonoids (107.52 ± 11.12 mg QE/g extract) which might be the reason of significant antioxidant and radical scavenging activities. Feeding of TILE (400 mg/kg/day) to HFD-fed rats increased activity of superoxide dismutase and catalase which is reflected as a significant reduction of oxidative stress markers like nitric oxide and malondialdehyde. TILE (400 mg/kg/day) feeding also down-regulated the mRNA levels of proadipogenic transcription factors including liver X receptor alpha (LXRα), peroxisome proliferator-activated receptor gamma (PPARγ), and sterol regulatory element-binding protein 1c (SREBP1c) in diet-induced obese rats. As a consequence of this, the mRNA level of lipogenic enzymes like acetyl-CoA carboxylase (ACC), fatty acid synthase (FAS), diacylglycerol acyltransferase (DGAT), and HMG-CoA reductase was down-regulated with a parallel up-regulation of the transcript level of lipolytic enzyme, hormone-sensitive lipase (HSL).

Conclusion
Observations from this study indicate that antioxidant-rich TILE can reduce HFD-induced body weight, fat weight and liver weight as well as blood lipids through down-regulating the gene expression of proadipogenic transcription factors and lipogenic enzymes with a concerted diminution of the gene expression of lipolytic enzyme, HSL.
Involving research participants in a pan-European research initiative: the EPAD participant panel experience
Authors: Gregory S. , Bunnik E. M. , Callado A. B. , Carrie I. , De Boer C. , Duffus J. , Fauria K. , Forster S. , Gove D. , Knezevic I. , Laquidain A. , Pennetier D. , Saunders S. , Sparks S. , Rice J. , Ritchie C. W. , Milne R. 
Published: October 2020

Abstract
Background
Including participants in patient and public involvement activities is increasingly acknowledged as a key pillar of successful research activity. Such activities can influence recruitment and retention, as well as researcher experience and contribute to decision making in research studies. However, there are few established methodologies of how to set up and manage participant involvement activities. Further, there is little discussion of how to do so when dealing with collaborative projects that run across countries and operate in multiple linguistic and regulatory contexts.

Methods
In this paper we describe the set-up, running and experiences of the EPAD participant panel. The EPAD study was a pan-European cohort study with the aim to understand risks for developing Alzheimer’s disease and build a readiness cohort for Phase 2 clinical trials. Due to the longitudinal nature of this study, combined with the enrolment of healthy volunteers and those with mild cognitive impairments, the EPAD team highlighted participant involvement as crucial to the success of this project. The EPAD project employed a nested model, with local panels meeting in England, France, Scotland, Spain and The Netherlands, and feeding into a central study panel. The local panels were governed by terms of reference which were adaptable to local needs.

Results
The impact of the panels has been widespread, and varies from feedback on documentation, to supporting with design of media materials and representation of the project at national and international meetings.

Conclusions
The EPAD panels have contributed to the success of the project and the model established is easily transferable to other disease areas investigating healthy or at-risk populations.
Serratus anterior plane block in modified radical mastectomy surgery: a case series
Authors: Datu Madonna Damayanthie , Prasetyadhi Jokevin 
Published: October 2020

AbstractBackgroundPostoperative breast pain may lead to poorer outcome if left untreated. Common analgesia modalities for postoperative breast pain include opioids and regional anesthesia. However, both of these modalities can cause significant side effects or complications. Serratus anterior plane (SAP) block is a new procedure that is relatively easier to perform and safer, compared with other modalities. Previous studies have reported its usefulness in reducing the need for both intraoperative and postoperative opioids.CaseWe reported 2 patients that underwent SAP block combined with general anesthesia in modified radical mastectomy (MRM). Patient 2 was given rescue analgesia during the intraoperative period. The administration of postoperative opioids did not exceed 24 h in both patients. Pain assessment using numeric rating scale (NRS) showed minimal postoperative pain. No side effects were found during 24-h monitoring period.ConclusionSAP block can be used as one of the modalities in managing the pain of MRM surgery.
Wisdom of crowds and collective decision-making in a survival situation with complex information integration
Authors: Hamada Daisuke , Nakayama Masataka , Saiki Jun 
Published: October 2020

Abstract
Background
The wisdom of crowds and collective decision-making are important tools for integrating information between individuals, which can exceed the capacity of individual judgments. They are based on different forms of information integration. The wisdom of crowds refers to the aggregation of many independent judgments without deliberation and consensus, while collective decision-making is aggregation with deliberation and consensus. Recent research has shown that collective decision-making outperforms the wisdom of crowds. Additionally, many studies have shown that metacognitive knowledge of subjective confidence is useful for improving aggregation performance. However, because most of these studies have employed relatively simple problems; for example, involving general knowledge and estimating values and quantities of objects, it remains unclear whether their findings can be generalized to real-life situations involving complex information integration. This study explores the performance and process of the wisdom of crowds and collective decision-making by applying the wisdom of crowds with weighted confidence to a survival situation task commonly used in studies of collective decision-making.

Results
The wisdom of crowds and collective decision-making outperformed individual judgment. However, collective decision-making did not outperform the wisdom of crowds. Contrary to previous studies, weighted confidence showed no advantage from comparison between confidence-weighted and non-weighted aggregations; a simulation analysis varying in group size and sensitivity of confidence weighting revealed interaction between group size and sensitivity of confidence weighting. This reveals that it is because of small group size and not the peculiarity of the survival task that results in no advantage of weighted confidence.

Conclusions
The study’s findings suggest that the wisdom of crowds could be applicable to complex problem-solving tasks, and interaction between group size and sensitivity of confidence weighting is important for confidence-weighted aggregation effects.
C-reactive protein course after classical complication free total knee arthroplasty using navigation
Authors: Nam Jun Ho , Cho Myung Rae , Lee Seo Ho , Song Suk-Kyoon , Choi Won-Kee 
Published: October 2020

Abstract
Purpose
The purpose is to estimate the degree of normalization of C-reactive protein (CRP) at 2 weeks and 4 weeks after uncomplicated total knee arthroplasty (TKA) using computer navigation. We also wish to determine whether the degree of normalization of CRP at 2 and 4 weeks differs after TKA performed in one knee and after TKA performed sequentially in both knees. We also want to analyze the patient factors that may influence the normalization of CRP.

Material and methods
We studied 400 knees who underwent primary computer-navigated TKA for treatment of advanced osteoarthritis: the TKAs were all performed by the same surgeon. We retrospectively analyzed CRP levels during the preoperative period, the early postoperative period (5–7 days), the 2-week postoperative period (12–14 days), and the 4-week postoperative period (25–30 days). We have assumed gender, age, body mass index (BMI), staged bilateral TKA, and preoperative CRP as the potential patient factors associated with CRP normalization.

Results
In unilateral TKA, CRP was normalized in 94 cases (34.3%) and in 219 cases (81.4%) within 2 weeks and 4 weeks after surgery, respectively. In second-knee, staged bilateral TKA, CRP was normalized in 46 cases (35.1%) and in 104 cases (79.4%) within 2 weeks and 4 weeks after surgery, respectively. There were no statistical differences between unilateral TKA and second-knee, staged bilateral TKA during the 2-week postoperative and the 4-week postoperative period. Compared to women, men were 1.99 times less likely to have normalized CRP at 2 weeks after surgery (P = 0.02).

Conclusion
CRP was less likely to normalize during the 2-week postoperative period in men than it is in women, while there was no difference between men and women in the normalization of CRP during the 4-week postoperative period. There were no statistical differences in the course of CRP levels after unilateral TKA and staged bilateral TKA during the 2-week postoperative and the 4-week postoperative period.
Lysinuric protein intolerance: an overlooked diagnosis
Authors: Olgac Asburce , Yenicesu Idil , Ozgul Rıza Köksal , Biberoğlu Gürsel , Tümer Leyla 
Published: October 2020

Abstract
Background
Lysinuric protein intolerance (LPI) is an autosomal recessively inherited inborn error of metabolism (IEM) caused by the defect in the dibasic cationic amino acid transporter found on the basolateral membrane of the lung, small intestine, and kidney due to mutations in the SLC7A7 gene, which encodes the y+LAT1 protein. LPI may present as an acute hyperammonemic episode or as chronic symptoms. Major clinical symptoms are feeding problems, vomiting and diarrhea, failure to thrive, hepatosplenomegaly, and cytopenia. We present a delayed diagnosis of symptomatic LPI with a homozygous mutation in the SLC7A7 gene.

Case presentation
A 15-year-old girl was referred to our clinic due to growth retardation and diarrhea. Physical examination showed short stature, retarded puberty, and hepatosplenomegaly. Laboratory tests showed normal complete blood count and biochemical analyses except elevated aspartate aminotransferase, triglyceride, total cholesterol, and ferritin. Peripheral blood smear and hemoglobin electrophoresis were within normal limits. Bone marrow analysis showed hemophagocytic cells. Postprandial ammonium level was found elevated. Low lysine, arginine, and ornithine and elevated glycine and alanine in plasma amino acid analysis and high amount of lysine and slightly elevated arginine and ornithine excretion in urine were detected. Molecular genetic analysis of the SLC7A7 gene showed a previously reported homozygous mutation. Low protein diet, sodium benzoate, l-carnitine, low-dose l-citrulline, and calcium replacement were initiated. The patient is now in good condition still being followed up in our department.

Conclusions
LPI is a metabolic disorder with multi-systemic involvement that may have severe consequences if left untreated. Initiation of early treatment is essential for the prevention of severe chronic complications. Also, confirmation of the genetic defect may provide the parents to have healthy offsprings in the future with the help of genetic counselling and preimplantation genetics.