bluuebunny/crossref_metadata_2025_split huggingface dataset:
Molecular basis for neofunctionalization of duplicated E3 ubiquitin ligases underlying adaptation to drought tolerance inArabidopsis thaliana
Authors: Jiménez‐Morales Estela , Aguilar‐Hernández Victor , Aguilar‐Henonin Laura , Guzmán Plinio 
Published: July 2020

SUMMARYMultigene families in plants expanded from ancestral genes via gene duplication mechanisms constitute a significant fraction of the coding genome. Although most duplicated genes are lost over time, many are retained in the genome. Clusters of tandemly arrayed genes are commonly found in the plant genome where they can promote expansion of gene families. In the present study, promoter fusion to theGUSreporter gene was used to examine the promoter architecture of duplicated E3 ligase genes that are part of group C in theArabidopsis thaliana ATLfamily. Acquisition of gene expression byAtATL78, possibly generated from defectiveAtATL81expression, is described.AtATL78expression was purportedly enhanced by insertion of a TATA box within the core promoter region after a short tandem duplication that occurred during evolution of Brassicaceae lineages. This gene is associated with an adaptation to drought tolerance ofA. thaliana. These findings also suggest duplicated genes could serve as a reservoir of tacit genetic information, and expression of these duplicated genes is activated upon acquisition of core promoter sequences. Remarkably, drought transcriptome profiling in response to rehydration suggests thatATL78‐dependent gene expression predominantly affects genes with root‐specific activities.
Network‐based approaches for understanding gene regulation and function in plants
Authors: Ko Dae Kwan , Brandizzi Federica 
Published: July 2020

SummaryExpression reprogramming directed by transcription factors is a primary gene regulation underlying most aspects of the biology of any organism. Our views of how gene regulation is coordinated are dramatically changing thanks to the advent and constant improvement of high‐throughput profiling and transcriptional network inference methods: from activities of individual genes to functional interactions across genes. These technical and analytical advances can reveal the topology of transcriptional networks in which hundreds of genes are hierarchically regulated by multiple transcription factors at systems level. Here we review the state of the art of experimental and computational methods used in plant biology research to obtain large‐scale datasets and model transcriptional networks. Examples of direct use of these network models and perspectives on their limitations and future directions are also discussed.
Fat graft for reducing pain in chronic wounds
Authors: Cuomo Roberto , Giardino Francesco R. , Nisi Giuseppe , Han Jingjian , Diluiso Giuseppe , Tresoldi Marco M. , Pieretti Gorizio , Brandi Cesare , Grimaldi Luca 
Published: July 2020

AbstractBackgroundChronic wounds are one of the most important challenge for regenerative surgery. Plastic surgeon can use fat graft to increase wound healing because its growth factors can enhance tissue regeneration. In a recent study, the authors evaluated a reduction of pain in a cohort of patients submitted to breast reconstruction with breast implant and lipofilling, putting into evidence that growth factors in fat graft can reduce post‐surgical pain. The aim of this work is to evaluate ultra‐filtered fat graft potential in reducing pain in chronic wounds.Patients and MethodsFifty new patients with chronic wounds of different etiology were recruited for this study and divided into two groups: A, treatment and B, control. Twenty‐five patients per group. Negative pressure therapy dressing was applied after surgical debridement. Three days later patients in group A received ultrafiltered fat graft. Pain was evaluated with preoperative Visual Analogic Scale, repeated twice a day for 14 days and finally 21 days from procedures.ResultsIn group A (treated patients), pain was lower. These data were confirmed even after 7 days. The overall statistical analysis of the average of all values (SD 1.72) confirmed that the differences were significant at the 95% with the Chi‐square test and analysis of variance (P value < .05).ConclusionsThe ultra‐filtered fat graft placed on the wound bed and edges was effective in reducing pain in chronic wounds. The reduction of pain was statistically significant.
Optical imaging of collagen fiber damage to assess thermally injured human skin
Authors: Schroeder Alexandra B. , Karim Aos , Ocotl Edgar , Dones Jesús M. , Chacko Jenu V. , Liu Aiping , Raines Ronald T. , Gibson Angela L. F. , Eliceiri Kevin W. 
Published: July 2020

AbstractSurgery is the definitive treatment for burn patients who sustain full‐thickness burn injuries. Visual assessment of burn depth is made by the clinician early after injury but is accurate only up to 70% of the time among experienced surgeons. Collagen undergoes denaturation as a result of thermal injury; however, the association of collagen denaturation and cellular death in response to thermal injury is unknown. While gene expression assays and histologic staining allow for ex vivo identification of collagen changes, these methods do not provide spatial or integrity information in vivo. Thermal effects on collagen and the role of collagen in wound repair have been understudied in human burn models due to a lack of methods to visualize both intact and denatured collagen. Hence, there is a critical need for a clinically applicable method to discriminate between damaged and intact collagen fibers in tissues. We present two complementary candidate methods for visualization of collagen structure in three dimensions. Second harmonic generation imaging offers a label‐free, high‐resolution method to identify intact collagen. Simultaneously, a fluorophore‐tagged collagen‐mimetic peptide can detect damaged collagen. Together, these methods enable the characterization of collagen damage in human skin biopsies from burn patients, as well as ex vivo thermally injured human skin samples. These combined methods could enhance the understanding of the role of collagen in human wound healing after thermal injury and potentially assist in clinical decision‐making.
Design and Performance Analysis of Mixed Flow Turbine Rotors With Extended Blade Chord
Authors: Leonard Thomas , Spence Stephen , Filsinger Dietmar , Starke Andre 
Published: July 2020

Abstract
Mixed flow turbines offer additional design freedom compared with conventional radial turbines. This is useful in the automotive turbocharger application to reduce rotor inertia, which can be very beneficial for the transient response of a highly boosted downsized passenger car powertrain. A previously published study from the authors analyzed a series of nine mixed flow turbine rotors with varying blade cone angle and inlet blade angle. This paper reports an extension of that study with two further mixed flow turbine rotors where the chord length of the rotor blade was extended. The aim of this work was to understand both the aerodynamic and mechanical impacts of varying the chord length, particularly for the turbocharger application where off-design performance and transient response are very important. The baseline mixed flow rotor for this study had a blade cone angle of 30 deg and an inlet blade angle of 30 deg. Two further variations were produced; one with the trailing edge (TE) extended in the downstream direction across the entire blade span. In the second variation, the chord was extended at the hub corner only, while the shroud corner of the TE remained unchanged, with the aim of achieving some aerodynamic improvement while meeting mechanical requirements. When the blade was extended at both the hub and shroud, the inertia and stress levels increased significantly and the blade eigenfrequencies reduced. There was a significant improvement in peak efficiency, but the mechanical performance was unfavourable. The improvement in peak efficiency was mainly due to better exhaust diffuser performance and, therefore, would not be realized in most turbocharger installations. The blade that was extended at only the hub corner incurred very little additional inertia, and the centrifugal stresses and blade eigenfrequencies were improved. Consequently, it was possible to reduce the blade thickness at the TE in order to achieve a more aerodynamically optimized design. In this case, the mechanical performance was acceptable and there were efficiency improvements of up to 1.1% points at off-design conditions, with no reduction in peak efficiency or maximum mass flowrate. Therefore, the blade that was extended only at the hub produced some improvement within acceptable mechanical limits. The flow field features were considered for the three rotor geometries to explain the changes in loss and efficiency across the operating range.
Carbohydrate Hydrogel Products Do Not Improve Performance or Gastrointestinal Distress During Moderate-Intensity Endurance Exercise
Authors: King Andy J. , Rowe Joshua T. , Burke Louise M. 
Published: July 2020

The benefits of ingesting exogenous carbohydrate (CHO) during prolonged exercise performance are well established. A recent food technology innovation has seen sodium alginate and pectin included in solutions of multiple transportable CHO, to encapsulate them at pH levels found in the stomach. Marketing claims include enhanced gastric emptying and delivery of CHO to the muscle with less gastrointestinal distress, leading to better sports performance. Emerging literature around such claims was identified by searching electronic databases; inclusion criteria were randomized controlled trials investigating metabolic and/or exercise performance parameters during endurance exercise >1 hr, with CHO hydrogels versus traditional CHO fluids and/or noncaloric hydrogels. Limitations associated with the heterogeneity of exercise protocols and control comparisons are noted. To date, improvements in exercise performance/capacity have not been clearly demonstrated with ingestion of CHO hydrogels above traditional CHO fluids. Studies utilizing isotopic tracers demonstrate similar rates of exogenous CHO oxidation, and subjective ratings of gastrointestinal distress do not appear to be different. Overall, data do not support any metabolic or performance advantages to exogenous CHO delivery in hydrogel form over traditional CHO preparations; although, one study demonstrates a possible glycogen sparing effect. The authors note that the current literature has largely failed to investigate the conditions under which maximal CHO availability is needed; high-performance athletes undertaking prolonged events at high relative and absolute exercise intensities. Although investigations are needed to better target the testimonials provided about CHO hydrogels, current evidence suggests that they are similar in outcome and a benefit to traditional CHO sources.
Different Time Course of Recovery in Achilles Tendon Thickness After Low-Load Resistance Training With and Without Blood Flow Restriction
Authors: Chulvi-Medrano Iván , Picón-Martínez Moisés , Cortell-Tormo Juan Manuel , Tortosa-Martínez Juan , Alonso-Aubin Diego Alexandre , Alakhdar Yasser 
Published: July 2020

Context: Blood flow restriction research has focused on muscular strength and hypertrophy. Limited data have been reported about the blood flow restriction effect on the tendon. Objective: To analyze and compare the time course of recovery in Achilles tendon thickness after a single bout of low-intensity resistance training (LI-RT) and low-intensity blood flow restriction training (LI-BFRT). Methods: A total of 56 healthy participants (24.60 [4.0] y; 23.65 [3.4] body mass index) were included. The dominant leg was assigned for LI-BFRT using low load (30% 1-repetition maximum) and 30% of the total occlusion pressure (52.21 [17.89] mm Hg) in plantar-flexion exercise (1 × 30 + 3 × 15 repetitions). The nondominant leg was assumed as a control condition. Main Outcome Measure: Sonography images were taken before the intervention, immediately posttraining, and 24 hours after exercise (post-24) for the Achilles tendon thickness. Results: Changes in Achilles tendon thickness for LI-BFRT group were significant post- (−14.5%; P < .05) and post-24 (−9.2%; P < .05). In contrast, LI-RT group showed a transient decrease after exercise (−9.67%; P < .05) followed by a recovery of thickness post-24 (−1.06%; P < .05). Thickness post-24 was different between LI-BFRT versus LI-RT (P < .01). Hedge effect size analysis showed a large effect (g = 0.90) in LI-BFRT pre–post condition and a medium effect (g = 0.57) in post- to post-24. The LI-RT obtained a medium effect (g = 0.53) in pre–post condition and a small effect (g = 0.49) in post- to post-24. Conclusions: This study showed a different time course of the acute response in Achilles tendon thickness between LI-BFRT and LI-RT. This may be associated with intratendinous fluid movement in response to LI-BFRT.
Clinical burden of diabetes in Italy in 2018: a look at a systemic disease from the ARNO Diabetes Observatory
Authors: Bonora Enzo , Cataudella Salvatore , Marchesini Giulio , Miccoli Roberto , Vaccaro Olga , Fadini Gian Paolo , Martini Nello , Rossi Elisa 
Published: July 2020

IntroductionDiabetes is a highly prevalent disease worldwide and represents a challenge for patients and healthcare systems. This population-based study evaluated diabetes burden in Italy in 2018 by assessing all aspects of outpatient and hospital care.Research design and methodsWe investigated data of 11 300 750 residents in local health districts contributing to ARNO Diabetes Observatory (~20% of Italian inhabitants). All administrative healthcare claims were analyzed to gather information on access to medical resources. Subjects with diabetes, identified by antihyperglycemic drug prescriptions, disease-specific copayment exemption and hospital discharge codes, were compared with age, sex and residency-matched non-diabetic individuals.ResultsWe identified 697 208 subjects with ascertained diabetes, yielding a prevalence of 6.2% (6.5% in men vs 5.9% in women, p<0.001). Age was 69±15 (mean±SD). As compared with non-diabetic subjects, patients with diabetes received more prescriptions of any drugs (+30%, p<0.001), laboratory tests, radiologic exams and outpatient specialist consultations (+20%, p<0.001) and were hospitalized more frequently (+86%, p<0.001), with a longer stay (+1.4 days, p<0.001). Although cardiovascular diseases accounted for many hospital discharge diagnoses, virtually all diseases contributed to the higher rate of hospital admissions in diabetic subjects (235 vs 99 per 1000 person-years, p<0.001). Healthcare costs were >2-fold higher in subjects with diabetes, mainly driven by hospitalizations and outpatient care related to chronic complications rather than to glucose-lowering drugs, diabetes-specific devices, or metabolic monitoring.ConclusionsThe burden of diabetes in Italy is particularly heavy and, as a systemic disease, it includes all aspects of clinical medicine, with consequent high expenses in all areas of healthcare.
Interventions to reduce inequalities in avoidable hospital admissions: explanatory framework and systematic review protocol
Authors: Sowden Sarah , Nezafat-Maldonado Behrouz , Wildman Josephine , Cookson Richard , Thomson Richard , Lambert Mark , Beyer Fiona , Bambra Clare 
Published: July 2020

IntroductionInternationally there is pressure to contain costs due to rising numbers of hospital admissions. Alongside age, socioeconomic disadvantage is the strongest risk factor for avoidable hospital admission. This equity-focussed systematic review is required for policymakers to understand what has been shown to work to reduce inequalities in hospital admissions, what does not work and where the current gaps in the evidence-base are.Methods and analysisAn initial framework shows how interventions are hypothesised to reduce socioeconomic inequalities in avoidable hospital admissions. Studies will be included if the intervention focusses exclusively on socioeconomically disadvantaged populations or if the study reports differential effects by socioeconomic status (education, income, occupation, social class, deprivation, poverty or an area-based proxy for deprivation derived from place of residence) with respect to hospital admission or readmission (overall or condition-specific for those classified as ambulatory care sensitive). Studies involving individuals of any age, undertaken in OECD (Organisation for Economic Co-operation and Development) countries, published from 2000 to 29th February 2020 in any language will be included. Electronic searches will include MEDLINE, Embase, CINAHL, Cochrane CENTRAL and the Web of Knowledge platform. Electronic searches will be supplemented with full citation searches of included studies, website searches and retrieval of relevant unpublished information. Study inclusion, data extraction and quality appraisal will be conducted by two reviewers. Narrative synthesis will be conducted and also meta-analysis where possible. The main analysis will examine the effectiveness of interventions at reducing socioeconomic inequalities in hospital admissions. Interventions will be characterised by their domain of action and approach to addressing inequalities. For included studies, contextual information on where, for whom and how these interventions are organised, implemented and delivered will be examined where possible.Ethics and disseminationEthical approval was not required for this protocol. The research will be disseminated via peer-reviewed publication, conferences and an open-access policy-orientated paper.PROSPERO registration numberCRD42019153666.
Comparison of visceral fat area measured by CT and bioelectrical impedance analysis in Chinese patients with gastric cancer: a cross-sectional study
Authors: Gao Bo , Liu Yu , Ding Chao , Liu Shunli , Chen Xiaotian , Bian Xiaojie 
Published: July 2020

ObjectivesBioelectrical impedance analysis (BIA) is a simple and inexpensive method to estimate body composition. However, the accuracy of BIA is unknown. We aimed to assess the accuracy of BIA in estimating visceral fat area (VFA) in patients with gastric cancer.Study designThis was a cross-sectional study comparing the accuracy of BIA in estimating VFA with the gold standard method measured by CT. VFA was measured in enrolled patients both by CT and BIA. VFA by CT at umbilical level ≥100 cm2 was considered as visceral obesity. Reliability between the two methods was assessed by intraclass correlation coefficient (ICC) and consistency was assessed by Bland-Altman method (95% limits of agreement). The area under the receiver operating characteristic curve (AUROC) was used to assess the performance of BIA in diagnosing visceral obesity.SettingThe study was conducted in China.ParticipantsFrom 1 January 2017 to 1 December 2018, a total of 157 patients diagnosed with gastric cancer were enrolled.ResultsOverall, VFA by CT and BIA in patients was 84.39±46.43 cm2 and 71.94±22.44 cm2, respectively. VFA estimated by BIA was positively correlated with VFA measured by CT using Pearson’s test (r=0.650, p<0.001). Overall, ICC for the two methods was 0.675. The mean bias between the two measurements was 12.45±36.13 cm2. The 95% limits of agreement ranged from −58.36 cm2 to 83.26 cm2. The cut-off value for diagnosing visceral obesity by BIA was 81 cm2 (AUROC: 0.822, p<0.001, 95% CI 0.758 to 0.887).ConclusionsVFA measured by BIA showed satisfactory reliability with that measured by CT. However, the absolute values of the two methods were not interchangeable. The cut-off value for VFA by BIA in diagnosing visceral obesity was 81 cm2 for patients with gastric cancer in the Chinese population.
Experiences of digital communication with automated patient interviews and asynchronous chat in Swedish primary care: a qualitative study
Authors: Entezarjou Artin , Bolmsjö Beata Borgström , Calling Susanna , Midlöv Patrik , Milos Nymberg Veronica 
Published: July 2020

ObjectivesTo explore staff experiences of working with a digital communication platform implemented throughout several primary healthcare centres in Sweden.DesignA descriptive qualitative approach using focus group interviews. Qualitative content analysis was used to code, categorise and thematise data.SettingPrimary healthcare centres across Sweden, in both rural and urban settings.ParticipantsA total of three mixed focus groups, comprising 19 general practitioners and nurses with experience using a specific digital communication platform.ResultsFive categories emerged: ‘Fears and Benefits of Digital Communication’, ‘Altered Practice Workflow’, ‘Accepting the Digital Society’, ‘Safe and Secure for Patients’ and ‘Doesn't Suit Everyone and Everything’. These were abstracted into two comprehensive themes: ‘Adjusting to a novel medium of communication’ and ‘Digitally filtered primary care’, describing how staff experienced integrating the software as a useful tool for certain clinical contexts while managing the communication challenges associated with written communication.ConclusionsFamily medicine staff were ambivalent concerning the use of digital communication but, after a period of adjustment, it was seen as a useful communication tool especially when combined with continuity of care. Staff acknowledged limitations regarding use by inappropriate patient populations, information overload and misinterpretation of text by both staff and patients.
Keeping afloat: a case study tracing the emergence of drowning prevention as a health issue in Bangladesh 1999–2017
Authors: Dodd Madeleine , Zwi Anthony , Rahman Aminur , Chowdhury Fazlul Kader , Ivers Rebecca Q , Jagnoor Jagnoor 
Published: July 2020

BackgroundDrowning is a leading cause of child death in Bangladesh. The present study investigated the emergence of drowning reduction as a priority within Bangladesh and the position it currently holds on the national policy agenda.MethodsThis case study documents the evolution of policy responses to drowning, reporting on data from semistructured interviews and a document analysis. To identify key factors that have facilitated the prioritisation of drowning prevention, data were synthesised using Shiffman and Smith’s 2007 Policy Prioritisation Framework. Furthermore, an inductive approach was used to identify key themes unique to drowning prevention that were not embedded in the framework.ResultsFour key phases of policy development for drowning prevention were distinguishable: (1) identification of issue and the emergence of actor support; (2) enhanced leadership and the accumulation of issue characteristics; (3) the formation of an internal frame and its impact on global support; and (4) enhanced national recognition and supportive global normative factors. Four additional themes unique to the case of drowning were also identified: competing health priorities, limited issue awareness, shift of disease burden to non-communicable diseases and the multisectoral nature of drowning.ConclusionThis study demonstrates that the level of political prioritisation given to drowning prevention has evolved over the last decade and a half. A comprehensive understanding of factors that have elevated the issue onto the policy agenda will ensure future stakeholder engagement activities can be designed to foster deeper and more sustained commitment by key actors and organisations.