bluuebunny/crossref_metadata_2025_split huggingface dataset:
Wybrane pomiary antropometryczne i MNA (mini nutritional assessment) a niektóre czynniki predykcyjne niedożywienia osób starszych
Authors: Głowacka Mariola , Haor Beata , Zabielska Paulina , Jurczak Anna , Biercewicz Monika , Jabłońska Renata , Karakiewicz-Krawczyk Katarzyna , Karakiewicz Beata 
Published: January 2020

ABSTRACTIntroduction: To a vast extent the body’s ageing process affects changes in the human nutritional status and body composition. The nutritional status of elderly people is assessed by means of nutritional history, physical examinations, somatometric measurements, biochemical and immunological tests. Moreover, survey tools (questionnaires, scales) are used in order to perform a screening and in-depth analysis of food intake and nutrition in the elderly. Today, an accurate assessment of body composition is conducted by means of specialist devices, such as those used for bioelectrical impedance analysis and dual X-ray absorptiometry.The objective of the thesis was to analyse the usage of selected anthropometric measurements in correspondence with an mini nutritional assessment (MNA) in studies on the identification of undernourishment in seniors, with consideration of some of the initiating factors of nourishment disorders in the population of the elderly.Methods: A review of articles considered essential from the point of view of the study objective was performed by analysing the PubMed database. The search query used was: “mini nutritional assessment MNA elderly”.Results: Nutritional status measured with the MNA may evaluate the way seniors feel. Overweight and obesity usually results in a worse assessment of the quality of the elderly’s life. A disordered nutritional status could stem from a range of medical, psychological and social factors.Conclusions: The results of anthropometric measurements are essential for an optimal identification of the risk of undernourishment in senior citizens by means of an MNA. They also complement information onthe initiating factors of eating disorders in the population of elderly people. In order to be able to widely use anthropometric measurements to complement knowledge on the nutritional status of the elderly, it is indispensable to conduct studies among healthy senior citizens to obtain information that will serve as reference data.
Problem ponoszenia kosztów związanych ze starzeniem się społeczeństwa – analiza na przykładzie domów pomocy społecznej w gminie miejskiej Szczecin
Authors: Giezek Marta , Iwański Rafał , Kożybska Marta , Zabielska Paulina , Paszkiewicz Monika , Karakiewicz-Krawczyk Katarzyna , Flaga-Gieruszyńska Kinga , Karakiewicz Beata 
Published: January 2020

ABSTRACTIntroduction: Selected aspects of securing the needs of dependent elderly persons by the social assistance sector from the perspective of law, society and economics, are presented in the article.The purpose of the article is to evaluate the sources of funding full-time long-term care services for the benefit of dependent elderly persons in Szczecin.Materials and methods: The analysis was based on statistical and financial data concerning the costs of maintenance in nursing homes in Szczecin provided for the benefit of dependent persons aged over 65.Results: Currently, the main costs of securing full-time care service needs fulfilled by social assistance institutions are borne by the city, which covers more than half of the expenses connected with care. One third of the cost is covered by the seniors themselves, while the involvement of families in payments does not exceed 5%.Conclusions: A reduction in the number of working age persons, increases in the number of elderly persons, an increasing demand for long-term care facilities, rising average annual costs of maintenance in nursing homes and the very low participation of families in those costs all result in the need to allocate greater amounts of funds in city budgets for providing care for dependent persons aged over 65.
Markedly Increased Ocular Side Effect Causing Severe Vision Deterioration After Chemotherapy Using New or Investigational Epidermal or Fibroblast Growth Factor Receptor Inhibitors.
Authors: Shin Eunhae , Lim Dong Hui , Han Jisang , Nam Do-Hyun , Park Keunchil , Ahn Myung-Ju , Kang Won Ki , Lee Jeeyun , Ahn Jin-Seok , Lee Se-Hoon , Sun Jong-Mu , Jung Hyun Ae , Chung Tae-Young 
Published: January 2020

Abstract
Background: We sought to describe corneal epithelial changes after using epidermal (EGFR) or fibroblast growth factor receptor (FGFR) inhibitors as chemotherapy and to clarify incidence and prognosis.
Materials : Retrospective chart review.
Results : Among 6,871 patients and 17 EGFR or FGFR inhibitors, 1,161 patients (16.9%) referred for ophthalmologic examination. In total, 1,145 patients had disease-related or unrelated ocular complications. Among 16 patients with treatment-related ocular complications, three patients had treatment-related radiation retinopathy and one patient showed treatment-related corneal ulcer. Finally the authors identified that, in 12 patients, three EGFR inhibitors and two FGFR inhibitors caused corneal epithelial lesions. Vandetanib, Osimertinib, and ABT-414 caused vortex keratopathy in nine patients, while ASP-5878 and FPA-144 caused epithelial changes resembling corneal dysmaturation in three patients. The mean interval until symptoms appeared was 246 days with vandetanib, 196 days with osimertinib, 30 days with ABT-414, 55 days with ASP-5878, and 70 days with FPA-144. The mean of the lowest logarithm of minimal angle of resolution visual acuity results of the right and left eyes after chemotherapy were 0.338 and 0.413. The incidence rates of epithelial changes were 15.79% with vandetanib, 0.005% with osimertinib, 100% with ABT-414, 50.0% with ASP-5878, and 18.2% with FPA-144. After excluding deceased patients and those who were lost to follow-up or still undergoing treatment, we confirmed the reversibility of corneal lesions after the discontinuation of each agent. Seven patients showed full recovery of their vision and corneal epithelium, while three achieved a partial level of recovery. Although patients diagnosed with glioblastoma used prophylactic topical steroids before and during ABT-414 therapy, all developed vortex keratopathy.
Conclusions: EGFR and FGFR inhibitors are chemotherapy agents that could make corneal epithelial changes. Contrary to the low probability of ocular complication with old EGFR drugs, recently introduced EGFR and FGFR agents showed a high incidence of ocular complication with severe vision distortion. Doctors should forewarn patients planning chemotherapy with these agents that decreased visual acuity could develop due to corneal epithelial changes and also reassure them that the condition could be improved after the end of treatment without the use of steroid eye drops.
Oxaliplatin resistance is enhanced by saracatinib via upregulation Wnt-ABCG1 signaling in hepatocellular carcinoma
Authors: Liao Xia , Song Ge , Xu Zihan , Bu Yang , Chang Fan , Jia Fengan , Xiao Xuelian , Ren Xuejiao , Zhang Mei , Jia Qingan 
Published: January 2020

Abstract
Background: Chemo-resistance in hepatocellular carcinoma (HCC) is a major problem, and acquired drug resistance prevents cancer therapies from achieving complete responses. Molecular targeting therapy presents an opportunity to impede tumor through combination or sequential therapy, while the accurate effect is vague.
Methods: The efficacy of combinations between oxaliplatin and anti-cancer molecular targeting drugs was screened. Strangely, the combined chemotherapy with oxaliplatin and saracatinib induced significantly antagonistic effects. Then the antitumor effects of combined treatment with saracatinib and oxaliplatin were confirmed in wide type HCC as well as in saracatinib- and oxaliplatin-resistant HCC. RNA sequencing was used to explore the resistance mechanism, and the roles of ABCG1 and Wnt signaling in oxaliplatin resistance were confirmed.
Results: Chemotherapy with oxaliplatin and saracatinib individually induced strong anti-HCC effects, while combined or sequential treatment of HCC cells with these two drugs exhibited reduced efficacy compared to treatment with the single drugs. And it was saracatinib treatment caused oxaliplatin resistance. RNA sequencing revealed 458 genes that were altered by treatment with saracatinib and oxaliplatin. Of these, the gene encoding the ATP-binding cassette transporter G1 ABCG1 and Wnt-associated genes were significantly upregulated. Upregulation of ABCG1 and oxaliplatin resistance were associated with activation of Wnt signaling. Interference with ABCG1 expression or inhibition of Wnt signaling resulted in reversal of the saracatinib-induced oxaliplatin resistance in HCC.
Conclusions: These studies demonstrated that combined or sequential chemotherapy with oxaliplatin and saracatinib reduced antitumor efficacy, and this antagonism was attributed to the activation of Wnt signaling and upregulation of ABCG1 by saracatinib.
Characterization of a vacuolar sucrose transporter, HbSUT5, from Hevea brasiliensis: involvement in latex production through regulation of intracellular sucrose transport in the bark and laticifers
Authors: long xiangyu , Li Heping , Yang Jianghua , Xin Lusheng , He Bin , Huang Debao , Tang Chaorong 
Published: January 2020

Abstract
Background: Sucrose (Suc), as the precursor molecule for rubber biosynthesis in Hevea brasiliensis , is transported via phloem-mediated long-distance transport from leaves to laticifers in trunk bark, where latex (cytoplasm of laticifers) is tapped for rubber. In our previous report, six Suc transporter (SUT) genes have been cloned in Hevea tree, among which HbSUT3 is verified to play an active role in Suc loading to the laticifers. In this study, another latex-abundant SUT isoform, HbSUT5 , with expressions only inferior to HbSUT3 was characterized especially for its roles in latex production.
Results: Both phylogenetic analysis and subcellular localization identify HbSUT5 as a tonoplast-localized SUT protein under the SUT4-clade (=type III). Suc uptake assay in baker’s yeast reveals HbSUT5 to be a typical Suc-H + symporter, but its high affinity for Suc (Km = 2.03 mM at pH 5.5) and the similar efficiency in transporting both Suc and maltose making it a peculiar SUT under the SUT4-clade. At the transcript level, HbSUT5 is abundantly and preferentially expressed in Hevea barks. The transcripts of HbSUT5 are conspicuously decreased both in Hevea latex and bark by two yield-stimulating treatments of tapping and ethephon, the patterns of which are contrary to HbSUT3. Under the ethephon treatment, the Suc level in latex cytosol decreases significantly, but that in latex lutoids (polydispersed vacuoles) changes little, suggesting a role of the decreased HbSUT5 expression in Suc compartmentalization in the lutoids and thus enhancing the Suc sink strength in laticifers.
Conclusions: Our findings provide insights into the roles of a vacuolar sucrose transporter, HbSUT5, in Suc exchange between lutoids and cytosol in rubber-producing laticifers.
Reliability and correlation of mixture cell correction in methylomic and transcriptomic blood data
Authors: Chaumette Boris , Kebir Oussama , Dion Patrick A. , Rouleau Guy A. , Krebs Marie-Odile 
Published: January 2020

Abstract
Objectives: DNA methylome and RNA transcriptome studies are growing, but investigators have to consider the cell type composition of tissues used. In blood samples, for instance, data reflect the picture of a mixture of cells. Specialized algorithms address the cell-type heterogeneity issue. We tested if the estimation of cell-type composition in the blood is correlated between two heterogeneous datasets. Results: We used methylome and transcriptome datasets obtained from a cohort of ten individuals whose blood was sampled at two different timepoints. We examined how the cell composition derived from these omics correlated with each other using “CIBERSORT” for transcriptome and “EstimateCellCounts function” for the methylome. The correlation coefficients between the two omic datasets range from 0.45 to 0.81. There was a minimal correlation between the two timepoints using the same algorithm. Our results suggest that a posteriori correction of a mixture of cells present in blood samples is reliable. Using an omic dataset to correct a second dataset for relative fractions of cells seems to be appropriate, but only when the samples are simultaneously collected. Such approaches could be beneficial when there are difficulties to control the cell types in the second dataset, even when the sample size is limited.
Differential expression of IDA (INFLORESCENCE DEFICIENT IN ABSCISSION)-like genes in Nicotiana benthamiana during corolla abscission, stem growth and water stress
Authors: Ventimilla Daniel , Domingo Concha , Gonzalez-Ibeas Daniel , Talon Manuel , Tadeo Francisco R. 
Published: January 2020

Abstract
Background
IDA (INFLORESCENCE DEFICIENT IN ABSCISSION)-like signaling peptides and the associated HAE (HAESA)-like family of receptor kinases were originally reported in the model plant Arabidopsis thaliana (Arabidopsis) to be deeply involved in the regulation of abscission. Actually, IDA peptides, as cell-to-cell communication elements, appear to be implicated in many developmental processes that rely on cell separation events, and even in the responses to abiotic stresses. However, the knowledge related to the molecular machinery regulating abscission in economically important crops is scarce. In this work, we determined the conservation and phylogeny of the IDA -like and HAE -like gene families in relevant species of the Solanaceae family and analyzed the expression of these genes in the allopolyploid Nicotiana benthamiana, in order to identify members involved in abscission, stem growth and in the response to drought conditions.
Results
The phylogenetic relationships among the IDA -like members of the Solanaceae studied, grouped the two pairs of NbenIDA1 and NbenIDA2 protein homeologs with the Arabidopsis prepropeptides related to abscission. Analysis of promoter regions searching for regulatory elements showed that these two pairs of homeologs contained both hormonal and drought response elements, although NbenIDA2A lacked the hormonal regulatory elements. Expression analyses showed that the pair of NbenIDA1 homeologs were upregulated during corolla abscission. NbenIDA1 and NbenIDA2 pairs showed tissue differential expression under water stress conditions, since NbenIDA1 homeologs were highly expressed in stressed leaves while NbenIDA2 homeologs, especially NbenIDA2B , were highly expressed in stressed roots. In non-stressed active growing plants, nodes and internodes were the tissues with the highest expression levels of all members of the IDA -like family and their putative HAE -like receptors.
Conclusion
Our results suggest that the pair of NbenIDA1 homeologs are involved in the natural process of corolla abscission while both pairs of NbenIDA1 and NbenIDA2 homeologs are implicated in the response to water stress. The data also suggest that IDA peptides may be important during stem growth and development. These results provide additional evidence that the functional module formed by IDA peptides and its receptor kinases, as defined in Arabidopsis, may also be conserved in Solanaceae.
LncRNA and transcriptomic analysis of fetal membrane reveal potential targets involved in oligohydramnios
Authors: Ou Yu-hua , Liu Yu-kun , Zhu Li-qiong , Chen Man-qi , Yi Xiao-chun , Chen Hui , Zhang Jian-ping 
Published: January 2020

Abstract
Background: The multiple causes of oligohydramnios make it challenging to study. Long noncoding RNAs (lncRNAs) are sets of RNAs that have been proven to function in multiple biological processes. The purpose of this study is to study expression level and possible role of lncRNAs in oligohydramnios.
Methods: In this study, total RNA was isolated from fetal membranes resected from oligohydramnios pregnant women (OP) and normal amount of amniotic fluid pregnant women (Normal). LncRNA microarray was used to analyze the differentially expressed lncRNAs and mRNAs. Kyoto Encyclopedia of Genes and Genomes (KEGG) was used to analyze the main enrichment pathways of differentially expressed mRNAs. Real-time quantitative PCR (qPCR) was used to validate the lncRNA expression level.
Results: LncRNA microarray analysis revealed that a total of 801 lncRNAs and 367 mRNAs were differentially expressed in OP; in these results, 638 lncRNAs and 189 mRNAs were upregulated, and 163 lncRNAs and 178 mRNAs were downregulated. Of the lncRNAs, 566 were intergenic lncRNA, 351 were intronic antisense lncRNA, and 300 were natural antisense lncRNA. The differentially expressed lncRNAs were primarily located in chromosomes 2, 1, and 11. KEGG enrichment pathways revealed that the differentially expressed mRNAs were enriched in focal adhesion as well as in the signaling pathways of Ras, TNF, estrogen, and chemokine. The qPCR results confirmed that LINC00515 and RP11-388P9.2 were upregulated in OP. Furthermore, the constructed lncRNA–miRNA–mRNA regulatory network revealed TNR, CFTR, ABCA12, and COL9A2 as the candidate targets of LINC00515 and RP11-388P9.2.
Conclusions:
In summary, we revealed the profiles of lncRNA and mRNA in OP. These results may offer potential targets for biological prevention for pregnant women with oligohydramnios detected before delivery and provide a reliable basis for clinical biological treatment in OP.
Characterization of wheat homeodomain-leucine zipper family genes and functional analysis of TaHDZ5-6A in drought tolerance in transgenic Arabidopsis
Authors: Li Shumin , Chen Nan , Li Fangfang , Mei Fangming , Wang Zhongxue , Cheng Xinxiu , Kang Zhensheng , Mao Hude 
Published: January 2020

Abstract
Background: Many studies in Arabidopsis and rice have demonstrated that HD-Zip transcription factors play important roles in plant development and responses to abiotic stresses. Although common wheat (Triticum aestivum L.) is one of the most widely cultivated and consumed food crops in the world, the function of the HD-Zip proteins in wheat is still largely unknown. Results: To explore the potential biological functions of HD-Zip genes in wheat, we performed a bioinformatics and gene expression analysis of the HD-Zip family. We identified 113 HD-Zip members from wheat and classified them into four subfamilies (I-IV) based on phylogenic analysis against proteins from Arabidopsis, rice, and maize. Most HD-Zip genes are represented by two to three homeoalleles in wheat, which are named as TaHDZX_ZA, TaHDZX_ZB, or TaHDZX_ZD, where X denotes the gene number and Z the wheat chromosome on which it is located. TaHDZs in the same subfamily have similar protein motifs and intron/exon structures. The expression profiles of TaHDZ genes were analysed in different tissues, at different stages of vegetative growth, during seed development, and under drought stress. We found that most TaHDZ genes, especially those in subfamilies I and II, were induced by drought stress, suggesting the potential importance of subfamily I and II TaHDZ members in the responses to abiotic stress. Compared with wild-type (WT) plants, transgenic Arabidopsis plants overexpressing TaHDZ5-6A displayed enhanced drought tolerance, lower water loss rates, higher survival rates, and higher proline content under drought conditions. Additionally, the transcriptome analysis identified a number of differentially expressed genes between 35S::TaHDZ5-6A transgenic and wild-type plants, many of which are involved in stress response. Conclusions: Our results will facilitate further functional analysis of wheat HD-Zip genes, and also indicate that TaHDZ5-6A may participate in regulating the plant response to drought stress. Our experiments show that TaHDZ5-6A holds great potential for genetic improvement of abiotic stress tolerance in crops.
Psychometric properties of the Adelaide Diagnostic Learning Inventory - Brief (ADLIB)
Authors: Minas Harry 
Published: January 2020

Abstract
Objective: There has been increased attention in recent years to mental health, quality of life, stress and academic performance among university students, and the possible influence of learning styles. Brief reliable questionnaires are useful in large-scale multivariate research designs, such as the largely survey-based research on well-being and academic performance of university students. The objective of this study was to examine the psychometric properties of a briefer version of the 39-item Adelaide Diagnostic Learning Inventory. Results: In two survey samples - medical and physiotherapy students - a 21-item version Adelaide Diagnostic Learning Inventory - Brief (ADLIB) was shown to have the same factor structure as the parent instrument, and the factor structure of the brief instrument was found to generalise across students of medicine and physiotherapy. Sub-scale reliability estimations were in the order of magnitude of the parent instrument. Sub-scale inter-correlations, inter-factor congruence coefficients, and correlations between ADLIB sub-scale scores and several external measures provide support support for the construct and criterion validity of the instrument.
Time to Change’s social marketing campaign for a new target population: results from 2017-2019.
Authors: González-Sanguino Clara , Potts Laura C , Milenova Maria , Henderson Claire 
Published: January 2020

Abstract
Background. Since 2009 Time to Change has included among its strategies a social marketing campaign to tackle the stigma surrounding mental health problems. At the start of its third phase (2016-2021) the target group of the campaign was kept as people between their mid-twenties and mid-forties but changed to middle-low income groups, with the content focused on men .
Methods. Participants (n=3700) were recruited through an online market research panel, before and after each burst of the campaign. They completed an online questionnaire evaluating knowledge (Mental Health Knowledge Schedule, MAKS); attitudes (Community Attitudes toward Mental Illness, CAMI); and desire for social distance (Intended Behaviour subscale of the Reported and Intended Behaviour Scale, RIBS). Socio-demographic data and awareness of the campaign were also collected.
Results. For each of the 3 bursts, significant pre-post awareness differences were found (OR=2.83, CI=1.90-4.20, p<0.001; OR=1.72, CI=1.22-2.42, p=0.002; OR=1.41, CI=1.01-1.97, p=0.043), and awareness at the end of the third burst was 33%. Demographic factors associated with awareness for one or more bursts included having children, familiarity with mental illness, male sex, being Black, Asian or other ethnic minorities and living in London or the East Midlands regions. An improvement across bursts in the “living with” subscale item of the RIBS, and in the “recover” and “advice to a friend” MAKS items were found. Familiarity with mental illness had the strongest association with all outcome measures, while the awareness of the campaign was also related with higher scores in MAKS and RIBS.
Conclusions. These interim results suggest that the campaign is reaching and having an impact on its new target audience to a similar extent as did the TTC phase 1 campaign. While over the course of TTC we have found no evidence that demographic differences in stigma have widened, and indeed those by age group and region of England have narrowed, those for socioeconomic status, ethnicity and sex have so far remained unchanged. By targeting a lower socioeconomic group and creating relatively greater awareness among men and in Black and ethnic minority groups, the campaign is showing the potential to address these persistent differences in stigma.