bluuebunny/crossref_metadata_2025_split huggingface dataset:
Elevated expression of FREM1 in breast cancer indicates favorable prognosis and high‐level immune infiltration status
Authors: Li Han‐ning , Li Xing‐rui , Lv Zheng‐tao , Cai Miao‐miao , Wang Ge , Yang Zhi‐fang 
Published: October 2020

AbstractBreast cancer (BC) poses one of the major threats to female's health worldwide. Immune infiltration in BC is a key representative of the tumor microenvironment and has been proven highly relevant for prognosis. The role of the FREM1 (FRAS1‐Related Extracellular Matrix 1) gene in carcinoma has not studied, moreover, the underlying mechanism remains largely unknown. This study aims to investigate the expression profile and potential action of FREM1 on BC progression. We applied series of bioinformatic methods as well as immunohistochemistry (IHC) and immunofluorescence (IF) to analyze FREM1 expression profile, its relationship with clinicopathological characteristics, impact on clinical outcomes, relevant functions, correlation with immune infiltration in BC. The results demonstrated that FREM1 had a dramatically reduced expression in BC tissues, possessed an inverse correlation with stage, age, and metastasis, and exhibited a higher level in invasive lobular breast carcinoma than in ductal one. Furthermore, decreased FREM1 expression was often associated with estrogen receptor (ER)/progesterone receptor (PR) negative and triple negative breast carcinoma (TNBC) status while human epidermal growth factor 2 (Her‐2) positive status, and considerably correlated with a worse overall survival (OS) and recurrence‐free survival (RFS). Meanwhile, the univariate/multivariate Cox model revealed that low‐FREM1 expression can be an independent prognostic factor for BC. Additionally, FREM1 was mainly involved in the cell metabolism and immune cells infiltration. Moreover, IHC and IF demonstrated a positive correlation of its expression with the immune infiltrating levels of CD4+, CD8+ T cells, and CD86+ M1 macrophages while a negative correlation with CD68+ pan‐macrophages and CD163+ M2 macrophages. These findings suggest that FREM1 can be a potential biomarker for evaluating the immune infiltrating status, and the BC prognosis.
Overexpression of miR‐329‐3p sensitizes osteosarcoma cells to cisplatin through suppression of glucose metabolism by targeting LDHA
Authors: Li Gang , Li Ye , Wang Da‐yong 
Published: October 2020

AbstractOsteosarcoma (OS) is one of the most frequent malignant bone tumor types. Traditional treatments of OS involve standard chemotherapy or combination with radiation before and after surgery. Cisplatin is one of the most effective chemotherapeutic drugs used for treating osteosarcoma. However, patients with advanced tumor stages develop cisplatin resistance, leading to a major clinical challenge. In this study, we investigated the roles of miR‐329‐3p in cisplatin sensitivity of osteosarcoma cells. We found miR‐329‐3p was significantly downregulated in osteosarcoma tissues compared with normal bone tissues. Overexpression of miR‐329‐3p suppressed osteosarcoma cell proliferation. Moreover, we observed low‐toxic cisplatin treatments suppressed miR‐329‐3p but higher concentrations of cisplatin‐induced miR‐329‐3p expression. In addition, miR‐329‐3p was significantly downregulated in cisplatin‐resistant Saos‐2 cells which displayed elevated glucose metabolism. Overexpression of miR‐329‐3p significantly impaired glucose metabolism of Saos‐2 cells. Bioinformatics analysis and luciferase assay consistently demonstrated the glycolysis enzyme, lactate dehydrogenase‐A (LDHA) was a direct target of miR‐329‐3p in osteosarcoma cells. Rescue experiments revealed restoration of LDHA in miR‐329‐3p‐overexpressed cisplatin‐resistant cells effectively recovered glucose metabolism, resulting in increased cisplatin resistance. This study demonstrates a miR‐329‐3p‐LDHA‐glucose metabolism‐cisplatin resistance axis in osteosarcoma cells, providing a miRNA‐based therapeutic strategy against chemoresistant osteosarcoma.
Contemporary transcatheter aortic valve implantation related thrombocytopenia
Authors: Abu Khadija Haitham , Ayyad Omar , Haberman Dan , Gandelman Gera , Poles Lion , Jonas Michael , Volodarsky Igor , Kweider Maher Abu , Maximovskih Alexander , Shimoni Sara , George Jacob , Blatt Alex 
Published: October 2020

AbstractBackgroundTranscatheter aortic valve implantation related thrombocytopenia (TAVI‐rTP) is an inevitable phenomenon. However, no study has been performed on TAVI‐rTP in the current setting of extended clinical indications combined with technology improvements.MethodsPatients with severe symptomatic aortic stenosis who underwent transfemoral TAVI, from January 2016 to December 2019 were enrolled.ResultsTwo‐hundred and one consecutive patients (mean age 81.1 ± 7.1 years, 96 men) enrolled. Platelet count was recorded before and after aortic valve implantation. Dropped platelet count (DPC) occurred in all but 11 patients who were included. Two groups were created: DPC <30 and DPC ≥30%. DPC was compared with in‐hospital major adverse cardiovascular events. The mean DPC was 26 ± 14%. We found that 67 patients (33.3%) had DPC of more or equal to 30%. In the univariable analysis, the time of the procedure (94.4 ± 32.7 vs 79.4 ± 21.9, p = .002), the amount of the contrast used (125.4 ± 55.4 vs 108.4 ± 44.1, p = .02) and the residual AV gradient (13.3 ± 7.2 vs 14.7, p = .05) were related to a DCP ≥30%. A DPC ≥30% was associated with increased risk of life‐threatening/major bleeding (11.9% vs 1.5%, p = .001), major vascular complications (16.4% vs 5.2% p = .009) and death (3.0% vs 0%, p = .044) at 30 days. After multivariable analysis, the factors associated with a higher DPC were time and contrast.ConclusionContemporary TAVI‐rTP in this cohort of patients continues to be a common phenomenon but severe thrombocytopenia is less frequent. Patients developing a DPC ≥30% are associated with poor outcomes at 30 days.
Progress in Effects of Microenvironment of Carbon‐based Catalysts on Hydrodeoxygenation of Biomass
Authors: Li Tong , Li Hao , Li Chunli 
Published: October 2020

AbstractCarbon‐based catalysts have made significant progress in hydrodeoxygenation (HDO) of biomass. Since the HDO reaction usually carried on surface of catalyst, the interaction of reactant with catalyst is closely related to the surface microenvironment of catalyst, which may further have an impact on catalytic performance. This review will discuss the relationship between surface microenvironment of carbon support and catalytic performance and mechanism of HDO reaction during biomass upgrading. We will focus on the microenvironment effect associated with HDO reaction in carbon‐based catalysts including effects of surface microstructure and surface functionalization of carbon supports on preparation of heterogeneous catalysts and catalytic performance, respectively. Study of microenvironment may provide guidance for further development of efficient carbon‐based catalysts in biomass resource utilization.
Sensory Perception of Non‐Deuterated and Deuterated Organic Compounds
Authors: Salthammer Tunga , Monegel Friederike , Schulz Nicole , Uhde Erik , Grimme Stefan , Seibert Jakob , Hohm Uwe , Palm Wolf‐Ulrich 
Published: October 2020

AbstractThe chemical background of olfactory perception has been subject of intensive research, but no available model can fully explain the sense of smell. There are also inconsistent results on the role of the isotopology of molecules. In experiments with human subjects it was found that the isotope effect is weak with acetone and D6‐acetone. In contrast, clear differences were observed in the perception of octanoic acid and D15‐octanoic acid. Furthermore, a trained sniffer dog was initially able to distinguish between these isotopologues of octanoic acid. In chromatographic measurements, the respective deuterated molecule showed weaker interaction with a non‐polar liquid phase. Quantum chemical calculations give evidence that deuterated octanoic acid binds more strongly to a model receptor than non‐deuterated. In contrast, the binding of the non‐deuterated molecule is stronger with acetone. The isotope effect is calculated in the framework of statistical mechanics. It results from a complicated interplay between various thermostatistical contributions to the non‐covalent free binding energies and it turns out to be very molecule‐specific. The vibrational terms including non‐classical zero‐point energies play about the same role as rotational/translational contributions and are larger than bond length effects for the differential isotope perception of odor for which general rules cannot be derived.
Chalice‐Type Tridentate Silicon Lewis Acids of C3 Symmetry in a Single Step Starting from Hexadehydrotribenzo[12]annulene
Authors: Schwartzen Anna , Weddeling Jan‐Henrik , Langosch Jana , Neumann Beate , Stammler Hans‐Georg , Mitzel Norbert W. 
Published: October 2020

AbstractTridentate Lewis acids with aligned functions were synthesized based on the rigid framework hexadehydrotribenzo[12]annulene. The backbone and its fluorinated analogue were synthesised in one‐pot syntheses, with alkyne deprotection and Sonogashira cross coupling reaction being carried out in one step. Hydrosilylation of the annulene with chlorohydrosilanes proceeded highly selectively and afforded rigid poly‐Lewis acids with three SiCl3 or SiCl2Me substituents perfectly oriented to one side of the molecule in a single step. The progress of hydrosilylation was investigated by time‐correlated NMR spectroscopic studies. The crystal structures show that the framework is symmetrically functionalised and the silyl substituents are aligned in one direction. To increase the acidity of the Lewis acids the chlorosilyl substituents were fluorinated with SbF3. Further investigation of hydrometallation reactions (M=B, Al, Ga, Sn) did not lead to corresponding structures.
A Bottom‐up In‐situ Preparation of Graphene‐like Porous Carbon for Ultrahigh Surface Area Specific Capacitance Supercapacitors
Authors: Hao Yisu , Liu Zheng , Chen Xingzhu , Liu Jingxiao , Tang Qunli , Hu Aiping , Chen Xiaohua 
Published: October 2020

AbstractNitrogen‐doped porous carbon is an ideal electrode material for supercapacitors, and the development of efficient and inexpensive graphene‐based porous carbon is of great significance to promote its application. In this study, a bottom‐up in‐situ method is developed to grow nitrogen‐doped 3D graphene‐like porous carbon (Cu‐GL‐NPC) with Cu as a catalyst. The Cu‐GL‐NPC possessed the high nitrogen content of 7.64% and the high ratio of pyrrolic nitrogen, along with open three‐dimensional porous structure, which led to the smooth transfer of electrolyte. Consequently, the Cu‐GL‐NPC delivered an extremely high surface area specific capacitance of 49.2 μF cm−2 at a current density of 1 A g−1. This study provides an insight for better understanding the effect of catalysts on the formation of porous carbon, which is beneficial for the design of new nanocarbon materials with different structures and different nitrogen doping types, and would offer ideas for the development of carbon materials for the next generation of supercapacitors.
Volunteering sustainability: An advancement in corporate social responsibility conceptualization
Authors: Farooq Qamar , Liu Xuan , Fu Peihua , Hao Yunhong 
Published: October 2020

AbstractIn order to meet the versatile expectations of stakeholders, enterprises need philanthropic representation in communities. This research aims to empirically investigate the employee‐specific viability of potential philanthropic initiatives of wholly foreign‐owned enterprises to delineate volunteering sustainability. It covers five potential internal and external volunteering initiatives—matchmaking, marriage arrangement, and poverty alleviation through skill development, anti‐sexual harassment awareness campaign and assistance to low paid workers, and finds the impact of intrinsic motivation for volunteering in proposed volunteering programs on employee's vital attitudes. By taking self‐determination theory, the extensive literature review of intrinsic motivation of employees and analysis of triple bottom line theory, the corporate social responsibility (CSR) and human resource management model proposed by Hao, Farooq, and Zhang (2018) has been empirically tested after collecting the data from 507 employees of sample wholly foreign‐owned enterprises.With ontological and epistemological assumptions in pragmatist stance, this research employed Qual plus Quan approach. HMLR was performed followed by PROCESS using SPSS for determining information technology usage moderation effects and partial mediation of organizational pride. The findings guide that moderated mediation effects of intrinsic motivation for volunteering in the same program are significant in the same direction for two employee attitudes; however, motivation has multifarious effects. The main theoretical implication is the CSR program specification of intrinsic motivation. The findings practically imply that companies should determine the intrinsic motivation of employees before designing volunteering programs. This research contributes to self‐determination theory by integrating the “people” tier of triple bottom line establishing the link of satisfaction to the intentional CSR activities.
Covalent Amine Tethering on Ketone Modified Porous Organic Polymers for Enhanced CO2 Capture
Authors: Jorayev Perman , Tashov Intizar , Rozyyev Vepa , Nguyen Thien S. , Dogan Nesibe A. , Yavuz Cafer T. 
Published: October 2020

AbstractEffective removal of excess greenhouse gas CO2 necessitates new adsorbents that can overcome the shortcomings of the current capture methods. To achieve that, porous materials are often modified post‐synthetically with reactive amine functionalities but suffer from significant surface area losses. Herein, we report a successful amine post‐functionalization of a highly porous covalent organic polymer, COP‐130, without losing much porosity. By varying the amine substituents, we recorded a remarkable increase in CO2 uptake and selectivity. Ketone functionality, a rarely accessible functional group for porous polymers, was inserted prior to amination and led to covalent tethering of amines. Interestingly, aminated polymers demonstrated relatively low heats of adsorption, which is useful for the rapid recyclability of materials, due to the formation of suspected intramolecular hydrogen bonding.
A novel autoantibody targeting calreticulin is associated with cancer in patients with idiopathic inflammatory myopathies
Authors: Chen He , Yang Heng , Cheng Qiu‐Xiang , Ge Yong‐Peng , Peng Qing‐Lin , Zhang Ya‐Mei , Cheng Gen‐Hong , Wang Guo‐Chun , Lu Xin 
Published: October 2020

AbstractObjectivesTo investigate the prevalence and clinical significance of anti‐calreticulin autoantibodies (anti‐CRT Ab) in a large cohort of idiopathic inflammatory myopathy (IIM) patients.MethodsSera from 469 patients with IIM, 196 patients with other connective tissue diseases, 28 patients with solid tumors and 81 healthy controls were screened for anti‐CRT Ab by enzyme‐linked immunosorbent assay using human recombinant CRT protein. Sera from 35 IIM patients were tested using an immunoprecipitation assay to confirm the presence of anti‐CRT Ab. Subsequently, IIM–cancer patients were identified and divided into new‐onset, remission and recurrent groups based on their cancer status. The relationships between anti‐CRT Ab levels and IIM disease activity were also investigated.ResultsSerum anti‐CRT Ab was detected positive in 81 of the 469 (17.3%) IIM patients. Immunoprecipitated bands were observed at a molecular weight of 60 kDa corresponding to the CRT protein. The IIM patients with anti‐CRT Ab more frequently had cancers compared to the patients without anti‐CRT Ab. Moreover, the prevalence of anti‐CRT Ab differed according to the cancer status. The IIM patients with recurrent cancers had a much higher prevalence of anti‐CRT Ab than those with cancers in remission. Also, serum anti‐CRT Ab levels positively correlated with disease activity at baseline and at follow‐up visits.ConclusionWe report the existence of serum anti‐CRT Ab in IIM patients and demonstrate the possible association of anti‐CRT Ab with malignancy in IIM patients. Serum anti‐CRT Ab could serve as a novel candidate marker of cancer in IIM patients.
Uncovering the secreted signals and transcription factors regulating the development of mammalian middle ear ossicles
Authors: Ankamreddy Harinarayana , Bok Jinwoong , Groves Andrew K. 
Published: October 2020

AbstractThe mammalian middle ear comprises a chain of ossicles, the malleus, incus, and stapes that act as an impedance matching device during the transmission of sound from the tympanic membrane to the inner ear. These ossicles are derived from cranial neural crest cells that undergo endochondral ossification and subsequently differentiate into their final functional forms. Defects that occur during middle ear development can result in conductive hearing loss. In this review, we summarize studies describing the crucial roles played by signaling molecules such as sonic hedgehog, bone morphogenetic proteins, fibroblast growth factors, notch ligands, and chemokines during the differentiation of neural crest into the middle ear ossicles. In addition to these cell‐extrinsic signals, we also discuss studies on the function of transcription factor genes such as Foxi3, Tbx1, Bapx1, Pou3f4, and Gsc in regulating the development and morphology of the middle ear ossicles.
Climate change has different predicted effects on the range shifts of two hybridizing ambush bug (Phymata, Family Reduviidae, Order Hemiptera) species
Authors: Zhang Vicki Mengyuan , Punzalan David , Rowe Locke 
Published: October 2020

AbstractAimA universal attribute of species is that their distributions are limited by numerous factors that may be difficult to quantify. Furthermore, climate change‐induced range shifts have been reported in many taxa, and understanding the implications of these shifts remains a priority and a challenge. Here, we use Maxent to predict current suitable habitat and to project future distributions of two closely related, parapatrically distributed Phymata species in light of anthropogenic climate change.LocationNorth America.TaxonPhymata americana Melin 1930 and Phymata pennsylvanica Handlirsch 1897, Family: Reduviidae, Order: Hemiptera.MethodsWe used the maximum entropy modeling software Maxent to identify environmental variables maintaining the distribution of two Phymata species, Phymata americana and Phymata pennsylvanica. Species occurrence data were collected from museum databases, and environmental data were collected from WorldClim. Once we gathered distribution maps for both species, we created binary suitability maps of current distributions. To predict future distributions in 2050 and 2070, the same environmental variables were used, this time under four different representative concentration pathways: RCP2.6, RCP4.5, RCP6.0, and RCP8.5; as well, binary suitability maps of future distributions were also created. To visualize potential future hybridization, the degree of overlap between the two Phymata species was calculated.ResultsThe strongest predictor to P. americana ranges was the mean temperature of the warmest quarter, while precipitation of the driest month and mean temperature of the warmest quarter were strong predictors of P. pennsylvanica ranges. Future ranges for P. americana are predicted to increase northwestward at higher CO2 concentrations. Suitable ranges for P. pennsylvanica are predicted to decrease with slight fluctuations around range edges. There is an increase in overlapping ranges of the two species in all future predictions.Main conclusionsThese evidences for different environmental requirements for P. americana and P. pennsylvanica account for their distinct ranges. Because these species are ecologically similar and can hybridize, climate change has potentially important eco‐evolutionary ramifications. Overall, our results are consistent with effects of climate change that are highly variable across species, geographic regions, and over time.
The balancing trick: Optimized sampling of imbalanced datasets—A brief survey of the recent State of the Art
Authors: Susan Seba , Kumar Amitesh 
Published: October 2020

AbstractThis survey paper focuses on one of the current primary issues challenging data mining researchers experimenting on real‐world datasets. The problem is that of imbalanced class distribution that generates a bias toward the majority class due to insufficient training samples from the minority class. The current machine learning and deep learning algorithms are trained on datasets that are insufficiently represented in certain categories. On the other hand, some other classes have surplus samples due to the ready availability of data from these categories. Conventional solutions suggest undersampling of the majority class and/or oversampling of the minority class for balancing the class distribution prior to the learning phase. Though this problem of uneven class distribution is, by and large, ignored by researchers focusing on the learning technology, a need has now arisen for incorporating balance correction and data pruning procedures within the learning process itself. This paper surveys a plethora of conventional and recent techniques that address this issue through intelligent representations of samples from the majority and minority classes, that are given as input to the learning module. The application of nature‐inspired evolutionary algorithms to intelligent sampling is examined, and so are hybrid sampling strategies that select and retain the difficult‐to‐learn samples and discard the easy‐to‐learn samples. The findings by various researchers are summarized to a logical end, and various possibilities and challenges for future directions in research are outlined.
Volatile constituents of yuzu (Citrus junos Sieb. ex Tanaka) peel oil: A review
Authors: Uehara Ayaka , Baldovini Nicolas 
Published: October 2020

AbstractYuzu (Citrus junos Sieb. ex Tanaka) is a very specific citrus variety mostly produced in Japan and South Korea. In recent years, it received considerable interest from the flavour and fragrance industry, and many studies devoted to the characterization of its volatile constituents have been published, often leading to somewhat contradictory results. This comprehensive review aims at giving a general view of the volatile components and the putative key odorants of Yuzu peel oil.
Astrocytes in the regulation of cerebrovascular functions
Authors: Cohen‐Salmon Martine , Slaoui Leila , Mazaré Noémie , Gilbert Alice , Oudart Marc , Alvear‐Perez Rodrigo , Elorza‐Vidal Xabier , Chever Oana , Boulay Anne‐Cécile 
Published: October 2020

AbstractAstrocytes are the most numerous type of neuroglia in the brain and have a predominant influence on the cerebrovascular system; they control perivascular homeostasis, the integrity of the blood–brain barrier, the dialogue with the peripheral immune system, the transfer of metabolites from the blood, and blood vessel contractility in response to neuronal activity. These regulatory processes occur in a specialized interface composed of perivascular astrocyte extensions that almost completely cover the cerebral blood vessels. Scientists have only recently started to study how this interface is formed and how it influences cerebrovascular functions. Here, we review the literature on the astrocytes' role in the regulation of the cerebrovascular system. We cover the anatomy and development of the gliovascular interface, the known gliovascular functions, and molecular factors, the latter's implication in certain pathophysiological situations, and recent cutting‐edge experimental tools developed to examine the astrocytes' role at the vascular interface. Finally, we highlight some open questions in this field of research.