bluuebunny/crossref_metadata_2025_split huggingface dataset:
Structural characterisation of a nanobody derived from a naïve library that neutralises SARS-CoV-2
Authors: Huo Jiangdong , Bas Audrey Le , Ruza Reinis R. , Duyvesteyn Helen M.E. , Mikolajek Halina , Malinauskas Tomas , Tan Tiong Kit , Rijal Pramila , Dumoux Maud , Ward Philip N. , Ren Jingshan , Zhou Daming , Harrison Peter J. , Radeke Julika , Zhao Yuguang , Gilbert-Jaramillo Javier , Knight Michael , Carrique Loic , Shah Pranav N.M. , James William , Townsend Alain , Stuart David I. , Owens Raymond J. , Naismith James H. 
Published: June 2020

Abstract
The SARS-CoV-2 virus is more transmissible than previous coronaviruses and causes a more serious illness than seasonal flu. The SARS-CoV-2 receptor binding domain (RBD) of the Spike protein binds to the human angiotensin-converting enzyme 2 (ACE2) receptor as a prelude to viral entry into the cell. Using a naïve llama single chain nanobody library and PCR maturation we have produced a nanobody, H11-D4, with a KD 9 nM for RBD that blocks the binding of RBD to the ACE2. Single particle cryo-electron microscopy revealed that H11-D4 binds to each of the three RBDs in the Spike trimer. The 1.8 Å crystal structure of the H11-D4 – RBD complex has illuminated the molecular interactions that drive the high affinity. H11-D4 binds to an epitope on RBD that overlaps with the ACE2 binding, explaining the blocking of ACE2 binding. The nanobody showed potent neutralising activity against live SARS-CoV-2 virus.
Oral Care for Neuroscience patients in New Zealand – A national survey
Authors: Woon Caroline 
Published: June 2020

Abstract

Aims: To understand the experience and knowledge of neuroscience nurses working in acute ward settings in New Zealand regarding oral care.
To determine what educational requirements were needed to standardise oral care.

Data sources: A systematic review of articles was conducted using Cinahl, PubMed, Cochrane and Google scholar between 2007-2019.

Methods: An online survey using Survey Monkey with three out of five units participating from the north and South Island of New Zealand using qualitative and quantitative data.

Results: 34% of nurses completed the survey from three different neuroscience wards. Oral hygiene education was provided to the majority of respondents during their initial nursing training, however this was considered inadequate and most nurses did not receive oral hygiene education since their training. It was considered that there was a lack of oral care assessment tools and guidelines available in New Zealand. A lack of evidence-based practice existed. Therefore, inconsistencies over products and frequency of care was problematic. Barriers to effective oral care included the uncooperative patient, lack of access to the mouth and a perceived lack of time to provide oral care.

Conclusion: The experience and knowledge of neuroscience nurses in this study was varied. An opportunity existed to implement an oral assessment tool and guideline which could improve the oral care of the neuroscience patient and standardize care throughout New Zealand. Oral hygiene education should be provided and available for nursing students, registered nurses, health care assistants, patients and family to ensure consistent effective oral care.

Impact: As a result of this study, a guideline and assessment flowchart were created with an online e-learning experience. This was distributed to a number of hospitals nationwide to ensure standardization of care across all neuroscience wards.
Effect of human urine application on cabbage production and soil characteristics
Authors: Alemayehu Yitayal Addis , Demoz Assefa Adane , Degefu Mekonnen Amberber , Gebreeyessus Getachew Dagnew , Demessie Sintayehu Fetene 
Published: May 2020

Abstract
The aim of this study was to examine the effect of human urine on soil quality and salinity through repeated applications, and compare it with the effects of synthetic fertilizer and non-fertilized treatments. Six different fertilizer mixtures were applied to pots of head cabbage: 100% tap water, 100% urine, 1:1 urine and tap water, 1:2 urine and tap water, 1:3 urine and tap water, and synthetic fertilizer. The study design was completely randomized design (CRD) with three replications of pot-based experiments. The growth and yield parameters of producing head cabbage (Brassica oleracea) among treatments were compared. Soil residual test of the optimum treatment was also conducted for the optimum treatment to examine the effect on its characteristics. The optimum yield was obtained from the application of 1:3 of urine and water, and comparable to the synthetic fertilizer (F = 21.78; p = 0.964). The difference in the electrical conductivity of soil was statistically significant (F = 2.324; p = 0.049) after three rounds of applications which should be considered during urine fertilizer utilization. Generally, urine contains nutrients, which can substitute synthetic fertilizer, at a dilution factor of three. However, education should be delivered to enhance public acceptance and to create awareness on urine collection, storage and application for sustainable utilization.
Integrated water management recommendations in practice: coexistence of old and new ways in Arizona
Authors: Berger Lena , Henry Adam Douglas , Pivo Gary 
Published: May 2020

AbstractIntegrated water management (IWM) is widely regarded as a key strategy in achieving a variety of urban sustainability goals. Despite the promise of this strategy, however, uptake of IWM practices has generally been slow. A central reason for this lies in the divergence of action recommendations in the literature and actual water management praxis. In this paper, we explore how action taken by governments relate (or not) to IWM dimensions found in the literature. We do this by combining a corpus of actions taken by local governments in Arizona with a systematic review of the IWM literature. More precisely, we identify a confined set of IWM action dimensions particularly relevant to current praxis and apply these to water management practices reported by local governments in Arizona. We find that governments in the state systematically use IWM strategies to complement or enhance traditional water management approaches. Uptake differs across management spheres in terms of magnitude and form and is informed by contextual characteristics. Overall, our study indicates that transition may be guided by bottom-up experimentation, context-sensitive selection, and incremental change. This is in contrast to how IWM is often understood in the literature – as sharp shift and break with old traditions.
Estresse percebido em familiares de pacientes em sala de espera de um centro cirúrgico
Authors: Pereira Portella Guerreiro Monique , Duarte Siqueira Fernanda , Matte Dezordi Catia Cristiane , Kirchner Rosane Maria , De Lima Dalmolin Graziele , Fernandes Stumm Eniva Miladi 
Published: May 2020

Objetivo: avaliar o estresse percebido de familiares de pacientes em sala de espera de um Centro Cirúrgico e relacioná-lo com variáveis sociodemográficas. Metodologia: estudo transversal com 104 familiares de pacientes submetidos à cirurgia que estavam na sala de espera de um Centro Cirúrgico. Foram utilizados o Formulário de Caracterização Sociodemográfica e Escala de Estresse Percebido. Resultados:. As maiores médias da Escala de Estresse Percebido pertencem às mulheres, maiores de 40 anos, sem companheiro, com filhos, que recebem menos de três salários mínimos e que cursaram o ensino médio, com diferença estatística significativa observada para variável sexo, exclusivamente. Em geral, os familiares encontravam-se em nível baixo de estresse. Conclusão: A espera em sala de Centro Cirúrgico pode contribuir com o surgimento de sentimentos como ansiedade, nervosismo e consequentemente estresse. É importante incluir o familiar no cuidado de enfermagem, que inclui a prestação de informações, diálogo e respeito.
GESTANTES QUE RECEBEM INFORMAÇÕES DE PROFISSIONAIS DE SAÚDE CONHECEM SEUS DIREITOS NO PERÍODO GRAVÍDICO-PUERPERAL
Authors: Junqueira Tainara Lucinda , Coelho Amanda Santos Fernandes , Sousa Marília Cordeiro de , Louro Nathalya Da Silva , Silva Priscila Salomão da , Almeida Nilza Alves Marques 
Published: May 2020

Objetivo: Associar a aquisição de conhecimento de gestantes sobre os direitos no período gravídico-puerperal com recebimento de informações por profissionais de saúde. Metodologia: Estudo transversal, descritivo, quantitativo, prospectivo, composto por 88 gestantes, no período de maio a agosto de 2018. Análise com teste de Qui-quadrado e Exato de Fisher. Resultados: Houve associação quanto quem recebeu informação e acertou sobre o período de licença maternidade (p=0.005722), sobre o direito de abono de falta no trabalho mediante apresentação do atestado médico e comparecimento (p=0.007903), e a lei do acompanhante (p=0.005869). Houve associação de quem não recebeu informação e errou sobre o direito de realizar no mínimo 6 consultas e demais exames complementares no horário de trabalho (p=0.02705). Conclusão: É de suma importância que as gestantes recebam orientações sobre os direitos por parte dos profissionais de saúde e exijam seu cumprimento, melhorando sua assistência obstétrica.Descritores: Gestantes, conhecimento, legislação, mulheres.
Videocase sobre a lista de verificação do parto seguro: sensibilização dos profissionais da saúde
Authors: Rodrigues Chagas da Costa Arlete , Mizusaki Imoto Aline , Bernarda Donato Gottems Leila 
Published: May 2020

RESUMOObjetivo: Descrever as etapas de produção e análise de conteúdo de um videocase para sensibilizar os profissionais de saúde sobre a Lista de Verificação do Parto Seguro. Metodologia: Estudo metodológico realizado a partir da construção de tecnologia educativa com a participação de nove especialistas. O método consistiu em duas etapas: elaboração do roteiro, gravação de entrevistas e de imagens reais em serviços de atenção ao parto e edição do vídeo; envio do vídeo aos participantes para confirmação e aprovação do uso dos trechos das falas selecionadas, e análise de conteúdo. Na análise dos dados, empregou-se o Índice de Validade de Conteúdo (IVC). Resultados: A duração do vídeo é de 15 minutos e 28 segundos, com aprovação de 100% dos especialistas no IVC. Conclusão: O vídeo pode ser utilizado na educação permanente dos profissionais de saúde: médicos, enfermeiros, e técnicos de enfermagem. Descritores: Lista de Checagem, Segurança do Paciente, Qualidade da Assistência à Saúde, Tecnologia educacional, Educação Permanente.
O processo de construção de material educativo para mulheres vítimas de violência
Authors: Schelb Márcia , De Oliveira Maria Liz Cunha , Gottems Leila Bernarda Donato , Soares Chariglione Isabelle Patriciá Freitas 
Published: May 2020

Objetivo: Decrever o processo de construção de um folder educativo sobre violência contra a mulher, destinada a promoção da saúde de mulheres. Metodologia: Utilizando-se o modelo da pesquisa-ação, a construção do folder contemplou quatro etapas: identificação do problema; preparação do conteúdo, baseado na literatura científica; construção gráfica do folder e revisão do material por pares. Resultados: O trabalho resultou na produção da versão final do material em formato de um folder, intitulado “Violência contra a mulher”. Discussão: A participação ativa dos profissionais e das mulheres vitimas de violência, com o uso de estratégia dialógica e coletiva, permeou o processo de construção do folder. As opiniões das mulheres e dos peritos, consideraram o folder como recurso adicional das atividades educativas, realizadas em qualquer serviço de atenção a mulher.
Enfermagem em Práticas Avançadas no atendimento pré-hospitalar:Oportunidade de ampliação do acesso no Brasil
Authors: Malvestio Marisa Aparecida Amaro , Behringer Lilian Prates Prates Belém , Martuchi Sérgio Dias , Fonseca Marcos Aurélio Da Silva , Da Silva Luciano , Souza Eduardo Fernando de , Hanszman Gilson Clementino , Bezerra Rildo 
Published: May 2020

O objetivo é analisar o cenário de implementação da EPA no atendimento pré-hospitalar (APH) como ferramenta de acesso ao cuidado de urgência no Brasil. Metodologia: Frente ao cenário das urgências, foi realizada análise da literatura relacionada a EPA e seus resultados, bem como investigação não-exaustiva sobre a participação do enfermeiro no APH em outros países, com vistas a explorar o impacto da introdução de enfermeiros na urgência pré-hospitalar no Brasil. Conclusão: A ampliação do papel dos enfermeiros no modelo brasileiro de APH pode viabilizar o alcance da cobertura 100% e incrementar a capacidade de avaliação e a realizaçãode procedimentos em tempo adequado, assegurando acesso oportuno ao cuidado. Decs: Serviços Médicos de Emergência; Prática Avançada de Enfermagem; Assistência à Saúde
Implantação da auditoria operativa em uma Autarquia Municipal
Authors: Liberatti Vanessa Moraes , Rafaeli Candido Pedro Danielli , Gvozd Raquel , Pissinati Paloma De Souza Cavalcante , Cunha Isabel Cristina Kowal , Haddad Maria do Carmo Fernandez Lourenço 
Published: May 2020

Objetivo: relatar a experiência do processo de implantação da auditoria operativa na Autarquia Municipal de Saúde de um município de grande porte do sul do Brasil. Método: trata-se de um relato de experiência, realizado por meio da análise de documentos internos e públicos da referida Autarquia de Saúde, elaborados durante os anos de 2004 a 2018. Resultados: identificou-se um aumento no número de auditores, ampliação da quantidade de serviços credenciados ao Sistema Único de Saúde que passaram a ser auditados, implantação e implementação de relatórios de auditoria, auxílio no desenvolvimento de manuais e protocolos, além de melhoria na qualidade da assistência prestada. Conclusão: a implantação do serviço de auditoria operativa é de suma importância na gestão do SUS, não só nos serviços de média e alta complexidade, mas também na atenção primária e demais setores e serviços de assistência à saúde.Descritores: Auditoria de Enfermagem; Sistema Único de Saúde; Enfermagem.
Educação em Enfermagem no Brasil: avanços e riscos
Authors: Ximenes Neto Francisco Rosemiro Guimarães 
Published: May 2020

A formação universitária em Enfermagem, tem passado por diversas e significativas mudanças e avanços a exemplo da Diretrizes Curriculares Nacionais, ampliação da carga horária da graduação em Enfermagem, para no mínimo de quatro mil horas, a integralização em cinco anos, currículos em tempo integral adotados por muitas Escolas, dentre outros. O ensino a distância representa um risco para as profissões da saúde, sobretudo para a Enfermagem, assim como para a população brasileira, sujeito de cuidado.
Whole Genome Re-sequencing of Soybean Accession EC241780 Providing Genomic Landscape of Candidate Genes Involved in Rust Resistance
Authors: Ratnaparkhe Milind Balkrishna , Marmat Niharika , Kumawat Giriraj , Shivakumar Maranna , Kamble Viraj Gangadhar , Nataraj Vennampally , Ramesh Shunmugiah Veluchamy , Deshmukh Milind Panjabrao , Singh Ajay Kumar , Sonah Humira , Deshmukh Rupesh Kailasrao , Prasad Manoj , Chand Suresh , Gupta Sanjay 
Published: June 2020

Background:
In this study, whole genome re-sequencing of rust resistant soybean genotype
EC241780 was performed to understand the genomic landscape involved in the resistance mechanism.

Methods:
A total of 374 million raw reads were obtained with paired-end sequencing performed with
Illumina HiSeq 2500 instrument, out of which 287.3 million high quality reads were mapped to Williams
82 reference genome. Comparative sequence analysis of EC241780 with rust susceptible cultivars
Williams 82 and JS 335 was performed to identify sequence variation and to prioritise the candidate
genes.

Results:
Comparative analysis indicates that genotype EC241780 has high sequence similarity with
rust resistant genotype PI 200492 and the resistance in EC241780 is conferred by the Rpp1 locus.
Based on the sequence variations and functional annotations, three genes Glyma18G51715, Glyma18G51741
and Glyma18G51765 encoding for NBS-LRR family protein were identified as the most
prominent candidate for Rpp1 locus.

Conclusion:
The study provides insights of genome-wide sequence variation more particularly at
Rpp1 loci which will help to develop rust resistant soybean cultivars through efficient exploration of
the genomic resource.
Extremophile Microbial Communities and Enzymes for Bioenergetic Application Based on Multi-Omics Tools
Authors: Fongaro Gislaine , Maia Guilherme Augusto , Rogovski Paula , Cadamuro Rafael Dorighello , Lopes Joana Camila , Moreira Renato Simões , Camargo Aline Frumi , Scapini Thamarys , Stefanski Fábio Spitza , Bonatto Charline , Souza Doris Sobral Marques , Stoco Patrícia Hermes , Duarte Rubens Tadeu Delgado , da Cruz Ariadne Cristiane Cabral , Wagner Glauber , Treichel Helen 
Published: June 2020

:
Genomic and proteomic advances in extremophile microorganism studies are increasingly
demonstrating their ability to produce a variety of enzymes capable of converting biomass into bioenergy.
Such microorganisms are found in environments with nutritional restrictions, anaerobic environments,
high salinity, varying pH conditions and extreme natural environments such as hydrothermal
vents, soda lakes, and Antarctic sediments. As extremophile microorganisms and their enzymes
are found in widely disparate locations, they generate new possibilities and opportunities to explore
biotechnological prospecting, including biofuels (biogas, hydrogen and ethanol) with an aim toward
using multi-omics tools that shed light on biotechnological breakthroughs.
The Antitumor Effects of Icaritin Against Breast Cancer is Related to Estrogen Receptors
Authors: Tao Cheng-Cheng , Wu Yue , Gao Xiang , Qiao Ling , Yang Ying , Li Fang , Zou Jie , Wang Yu-Hao , Zhang Shu-Yu , Li Chang-Long , Zhang Yuan-Yuan , Sun Xiao-Dong 
Published: May 2020

Objective:
We aim to investigate the anticancer effects and mechanisms of
icaritin against breast cancer.

Materials and Methods:
Both estrogen receptor (ER) positive breast cancer cells MCF-
7 and ER-negative MDA-MB-231 cells were employed. We examined the effects of
icaritin on the proliferation and migration by wound healing assay and transwell assay.
Cell apoptosis and cell cycle of MCF-7 and MDA-MB-231 cells were analyzed using
Flow cytometry. Cell autophagy of MCF-7 and MDA-MB-231 cells was assessed by
western blotting, acridine orange staining and confocal microscopy. We also detected
the expression of apoptosis-related genes by western blotting. In addition, an autophagy
inhibitor was used to investigate whether cytoprotective autophagy was induced.
Meanwhile, an ER inhibitor was utilized to explore whether ER was involved in
autophagy.

Results:
Icaritin inhibited the proliferation and migration, and induced cell cycle arrest of
both MDA-MB-231 and MCF-7 cells. Icaritin significantly induced apoptosis of MDA-MB-
231 cells by activating caspase-3. And icaritin stimulated autophagy in MCF-7 cells, as
evidenced by increased LC3II/LC3I, enhanced p62 degradation, the accumulation of
endogenous LC3 puncta formation, and the increased autophagy flux. Icaritin induced
autophagy through upregulating the phosphorylation of AMPK and ULK1. Chloroquine,
an autophagy inhibitor, increased icaritin-induced apoptosis and proliferation inhibition of
MCF-7 cells. Meanwhile, tamoxifen, an ER inhibitor, reversed icaritin-induced autophagy
and proliferation inhibition of MCF-7 cells.

Conclusion:
Our study demonstrated that the antitumor effects of icaritin against breast
cancer are related to ER, which suggested that the status of ER should be considered in
the clinical application of icaritin.
Development of Sustained Release Oseltamivir Phosphate Dry Powder Inhaler: In-Vitro Characterization and In-Vivo Toxicological Studies
Authors: Sahastrabudhe Harshal , Kenjale Prathmesh , Pokharkar Varsha 
Published: May 2020

Background:
Oseltamivir Phosphate (OP) is an ethyl ester prodrug prescribed for the treatment
of influenza virus infection. Current marketed formulations of OP have been observed to be supplemented
with an adverse effect during post-marketing surveillance. These prerequisites are sufficed
by developing a sustained release Dry Powder for Inhalation (DPI).

Objectives:
The objective of the present study was to develop OP-DPI by an innovative formulation
approach comprising of Immediate (IR) and Sustained (SR) Release portions.

Methods:
DPI formulation comprising IR and SR portions were prepared by spray drying technique
using Hydroxy Propyl Methyl Cellulose (HPMC) as the rate-controlling polymer for SR portion. The
spray-dried product was further characterized for various pharmaco-technical, in-vitro and in-vivo parameters.

Results:
OP-DPI showed a burst release of 49% within 15 min further sustaining the drug release up to
9 hrs. The in-vitro aerodynamic performance of OP-DPI showed maximum deposition at stage 3 and
Fine Particle Dose (FPD) of 1.08 mg indicating deposition in the upper respiratory tract. Solid-state
characterization by DSC and XRD indicated the partial amorphization of OP due to spray drying. In-vivo
toxicological examination revealed no sign of inflammation, indicating the safety of the developed
formulation. Accelerated stability study as per ICH guidelines displayed no significant change in the
solid-state characterization and drug-related performance of OP-DPI.

Conclusion:
Prepared novel and scalable OP-DPI may have the potential to overcome the problems
associated with existing marketed dosage forms of OP. Further, localized drug delivery of the antiviral
drug through the pulmonary route might be clinically beneficial in controlling the viral proliferation.
Polycystic Ovary Syndrome and Probiotics: A Natural Approach to an Inflammatory Disease
Authors: Schiattarella Antonio , Riemma Gaetano , La Verde Marco , Franci Gianluigi , Chianese Annalisa , Fasulo Diego , Fichera Michele , Gallo Pasquale , De Franciscis Pasquale 
Published: June 2020

:
Polycystic ovary syndrome (PCOS) is a condition that affects about 15% of women of
reproductive age and is correlated with infertility, insulin resistance, and obesity. The etiology of
PCOS is multifactorial and genetic, endocrine, and metabolic causes are involved.

:
New evidence suggests a link between microorganisms residing in the digestive tracts of humans
and the development of PCOS. Moreover, an imbalance in the gut microbial community could be a
possible factor for the onset of insulin resistance and obesity. Hyperandrogenism, a key feature of
PCOS, could also play a critical role in shaping the microbiome community.

:
Probiotics could modify the gut microbiota and serve as a potential treatment for PCOS. Here we
disclose the association between PCOS and intestinal microbiota and the possible role of probiotics
as a new treatment approach.