bluuebunny/crossref_metadata_2025_split huggingface dataset:
Characterization of mesenchymal heart cells obtained from patients with tetralogy of Fallot and ventricular septal defect
Authors: Kozyrev I. A. , Golovkin A. S. , Ignatieva E. S. , Dokshin P. M. , Grekhov E. V. , Gordeev M. L. , Pervunina T. M. , Kostareva A A. , Malashicheva A. B. 
Published: November 2019

Objective. Phenotypic analysis of cardiac mesenchymal cells from patients with a ventricular septal defect and tetralogy of Fallot.Methods. The study included cardiac mesenchymal cells from 8 patients with ventricular septal defect and from 18 patients with tetralogy of Fallot who underwent surgery. Using the flow cytometry method, the content of the following antigens on the cell surface was evaluated: CD31, CD34, CD90, CD117, CD146, CD166, PDGFRB.Results. Cardiac mesenchymal cells from patients with ventricular septal defect are statistically significantly different from patients with tetralogy of Fallot in surface markers CD90 and PDGFRB. The content of a stem cell marker PDGFRB tends to decrease with age in patients with tetralogy of Fallot.Conclusion. The cells obtained from myocardial tissue from patients with tetralogy of Fallot and from patients with ventricular septal defects have characteristics of mesenchymal stem cells. The differences found between the cells from the two groups of patients indicate that pathology can affect the phenotype of cardiac mesenchymal cells. Apparently, the stem properties of the mesenchymal heart cells tend to decrease with age.
Performance of Intergrowth 21st Growth Charts in the Diagnosis of Small and Large for Gestational Age in Term and Preterm Newborns
Authors: Marques Bárbara , Martins Rosa , Rodrigues Teresa , Oliveira Graça , Abrantes Margarida 
Published: November 2019

Introduction: Birth weight is a major contributor to neonatal morbidity and mortality and is associated with chronic diseases in adulthood. This study aimed to evaluate the use of Intergrowth 21st instead of the Fenton & Kim 2013 growth charts in the diagnosis of small and large for gestational age in a group of Portuguese newborns.Material and Methods: We conducted an analytical and retrospective study to evaluate birth weight of term and preterm newborns using both growth charts. Groups studied: ‘Term-weeks’ and ‘Term-days’ (term newborns with gestational age in weeks and days, respectively), ‘Preterm-weeks’ and ‘Preterm-days’ (preterm newborns with gestational age in weeks and days, respectively).Results: A total of 14 056 newborns were included, 6% preterm. Using the Intergrowth 21st growth charts, the groups ‘Term-weeks’ (n = 12 081), ‘Term-days’ (n = 1118), ‘Preterm-weeks’ (n = 617) and ‘Preterm-days’ (n = 240), classified as small for gestational age according to the Fenton & Kim 2013 growth charts were adequate for gestational age in 52.8%, 57.8%, 37.7% and 9.3% respectively; and 9.2%, 9.2%, 5.9% and 0.6% of adequate for gestational age newborns were large for gestational age, respectively. In the ‘Pretermdays’ group, 7.9% of adequate for gestational age newborns were small for gestational age and 22.2% of large for gestational age newborns were adequate for gestational age, all with gestational age below 231 days.Discussion: The use of the Intergrowth 21st growth charts in this sample resulted in a lower number of newborns being classified as small for gestational age, except in very preterm newborns.Conclusion: Considering the results obtained, we suggest that Portuguese maternity hospitals use the Intergrowth 21st instead of the Fenton & Kim 2013 growth charts. However, more studies are needed to confirm these results.
Ex-vivo Surgical Repair of a Renal Artery Aneurysm with Kidney Autotransplantation
Authors: Sobrinho Gonçalo , Ministro Augusto , Silva Artur , Pedro Luís 
Published: November 2019

Renal artery aneurysms are rare. They are most commonly degenerative, congenital or due to medial fibroplasia. Proximal aneurysms can be repaired by endovascular and in-situ surgical techniques. However, aneurysms of the distal renal artery and its branches require ex-vivo surgical repair, also known as auto-transplantation: the kidney is removed, dissected and reconstructed in cold ischemia, and put back in place. A 69-year-old woman, with hypertension, presented with bilateral renal artery aneurysms with a diameter of 3.4 cm on the right kidney and 1 cm on the left kidney. The right renal artery aneurysm, which was due to medial fibroplasia, was successfully repaired using the ex-vivo surgical technique. Patency was confirmed by postoperative computed tomography angiography.
Reninoma: Uma Causa Rara de Hipertensão Endócrina
Authors: Marques Bernardo , Couto Joana , Lemos Manuel C. , Rodrigues Fernando 
Published: November 2019

Os tumores justa-glomerulares são causas raras de hipertensão arterial de difícil controlo e cursam habitualmente com hipocaliémia, hiperreninémia e hiperaldosteronismo secundário. Descreve-se o caso de uma doente, de 45 anos, com história pessoal de hipertensão arterial de difícil controlo associada a hipocaliémia desde os 35 anos, medicada com quatro classes de anti-hipertensores. Do estudo realizado destaca-se hiperaldosteronismo secundário [aldosterona 44,3 ng/dL (4 - 28 ng/dL), renina > 1000 mUI/mL (4,4 - 46,16 mUI/mL)] e tomografia axial computorizada, que identificou formação nodular heterogénea localizada no terço médio do rim direito, com 3,7 cm de diâmetro. Foi realizada nefrectomia parcial, cuja análise histológica confirmou o diagnóstico de reninoma. Após a cirurgia, verificou-se normalização dos doseamentos hormonais (aldosterona 9,2 ng/dL; renina 1,20 mUI/mL) e da pressão arterial. Pretende-se chamar a atenção para esta causa potencialmente curável de hipertensão arterial endócrina. A ressecção cirúrgica é o tratamento de escolha e leva à normalização da pressão arterial.
Mapping the spread of Avian Influenza Virus in Poultry at Polewali Mandar Regency, 2008-2013
Authors: Thaha Aminah Hajah , Rauf Juliawati , Bagenda Isnaniah 
Published: November 2019

The spread of Avian Influenza (AI) virus have a significant impact on the decline in the bird population, economic losses, and food safety. The purpose of this study was to determine level and patterns of distribution AI in chicken at Polewali Mandar Regency, period 2008-2013. The results showed that in 2008 to 2013 cases of poultry deaths by AI has spread in 13 districts of the total 16 districts and 53 villages of the total 167 villages in Polewali Mandar Regency. It shows that 81,25% of district and 31,74% of villages in Polewali Mandar has been infected with AI. The number of deaths of poultry caused AI period 2008 to 2013 is 49,182. The chicken that are infected native chicken, broiler, ornamental chickens (Philipin), cuckoo, rooster, and ducks. The chicken reared in backyard and commercial poultry sector 3 with the number of chicken are 10-10000. Trend increase in cases of poultry death by AI period 2008 to 2013 in Polewali Mandar generally occurs in January, February, March, July, September, November, and December.
Phenotypic Analysis Combined with Tandem Mass Tags (TMT) Labeling Reveal the Heterogeneity of Strawberry Stolon Buds
Authors: Guan Ling , Zhao Mizhen , Qian Yaming , Yu Hongmei , Xia Jin , Wu Ejiao 
Published: November 2019

Abstract
Background: Ramet propagation in strawberry (Fragaria × ananassa) is the most effective way in production. However, the lack of systematically phenotypic observations and high-throughput methods limits our ability to analyze the key factors regulating the heterogeneity in strawberry stolon buds. Results: From observation, we found that the axillary bud located in the first node quickly stepped into dormancy (DSB), after several bract and leaf buds were differentiated. The stolon apical meristem (SAM) degenerated as the new ramet leaf buds (RLB), and the new active axillary stolon buds (ASB) differentiated continually after the differentiation of the first leaf. Using the tandem mass tags (TMT) labeling method, a total of 7,271 strawberry proteins were identified. Between ASB and DSB, the spliceosome DEPs, such as Ser/Arg-rich (SR) and heterogeneous nuclear ribonucleoprotein particle (hnRNP), showed the highest enrichment and high PPI connectivity. This indicated that the differences in DEPs (e.g., SF-3A and PK) at the transcriptional level may be causing the differences between the physiological statuses of ASB and DSB. As expected, the photosynthetic pre-form RLB mainly differentiated from ASB and DSB judging by the DEP enrichment of photosynthesis. However, there are still other specialized features of DEPs between RLB and DSB and between ASB and DSB. The DEPs relative to DNA duplication [e.g., minichromosome maintenance protein (MCM 2, 3, 4, 7)], provide a strong hint of functional gene duplication leading the bud heterogeneity between RLB and DSB. In addition, the top fold change DEP of LSH 10-like might be involved in the degeneration of SAM into RLBs, based on its significant function in modulating the plant shoot initiation. As for RLB/ASB, the phenylpropanoid biosynthesis pathway probably regulates the ramet axillary bud specialization, and further promotes the differentiation of xylem when ASB develops into a new stolon [e.g., cinnamyl alcohol dehydrogenase 1 (CAD1) and phenylalanine ammonia-lyase 1 (PAL1)]. Conclusions: By using phenotypic observation combined with proteomic networks with different types of strawberry stolon buds, the definite dormancy phase of DSB was identified, and the biological pathways and gene networks that might be responsible for heterogeneity among different stolon buds in strawberry were also revealed.
The pathogen Moniliophthora perniciosa promotes differential proteomic modulation of cacao genotypes with contrasting resistance to witches´ broom disease
Authors: Santos Everton Cruz , Pirovani Carlos Priminho , Correa Stephany , Micheli Fabienne , Gramacho Karina P 
Published: November 2019

Abstract
Background: Witches’ broom disease (WBD) of cacao (Theobroma cacao L.), caused by Moniliophthora perniciosa, is the most important limiting factor for the cacao production in Brazil. Hence, the development of cacao genotypes with durable resistance is the key challenge for control the disease. Proteomic methods are often used to study the interactions between hosts and pathogens, therefore helping classical plant breeding projects on the development of resistant genotypes. The present study compared the proteomic alterations between two cacao genotypes standard for WBD resistance and susceptibility, in response to M. perniciosa infection at 72 hours and 45 days post-inoculation; respectively the very early stages of the biotrophic and necrotrophic stages of the cacao x M. perniciosa interaction.

Results: A total of 554 proteins were identified, being 246 in the susceptible Catongo and 308 in the resistant TSH1188 genotypes. The identified proteins were involved mainly in metabolism, energy, defense and oxidative stress. The resistant genotype showed more expressed proteins with more variability associated with stress and defense, while the susceptible genotype exhibited more repressed proteins. Among these proteins, stand out pathogenesis related proteins (PRs), oxidative stress regulation related proteins, and trypsin inhibitors. Interaction networks were predicted, and a complex protein-protein interaction was observed. Some proteins showed a high number of interactions, suggesting that those proteins may function as cross-talkers between these biological functions.

Conclusions: We present the first study reporting the proteomic alterations of resistant and susceptible genotypes in the T. cacao x M. perniciosa pathosystem. The important altered proteins identified in the present study are related to key biologic functions in resistance, such as oxidative stress, especially in the resistant genotype TSH1188, that showed a strong mechanism of detoxification. Also, the positive regulation of defense and stress proteins were more evident in this genotype. Proteins with significant roles against fungal plant pathogens, such as chitinases, trypsin inhibitors and PR 5 were also identified, and they may be good resistance markers. Finally, important biological functions, such as stress and defense, photosynthesis, oxidative stress and carbohydrate metabolism were differentially impacted with M. perniciosa infection in each genotype.
Nasopharyngeal carriage, spa types and antibiotic susceptibility profiles of Staphylococcus aureus from healthy children less than 5 years in Eastern Uganda
Authors: Kateete David , Asiimwe Benon B , Mayanja Raymond , Mujuni Brian , Bwanga Freddie , Najjuka Christine F , Källander Karin , Rutebemberwa Elizeus 
Published: November 2019

Abstract
Background:
Staphylococcus aureus carriage is a known risk factor for staphylococcal disease. However, the carriage rates vary by country, demographic group and profession. This study aimed to determine the S. aureus carriage rate in children in Eastern Uganda, and identify S. aureus lineages that cause infection in Uganda.

Methods:
Nasopharyngeal samples from 742 healthy children less than 5 years residing in the Iganga/Mayuge Health and Demographic Surveillance Site in Eastern Uganda were processed for isolation of S. aureus. Antibiotic susceptibility testing based on minimum inhibitory concentrations (MICs) was determined by the BD PhoenixTM system. Genotyping was performed by spa and SCCmec typing.

Results:
The processed samples yielded 144 S. aureus isolates (one per child) therefore, the S. aureus carriage rate in children was 19.4% (144/742). Thirty one percent (45/144) of the isolates were methicillin resistant (MRSA) yielding a carriage rate of 6.1% (45/742). All isolates were susceptible to rifampicin, vancomycin and linezolid. Moreover, all MRSA were susceptible to vancomycin, linezolid and clindamycin. Compared to methicillin susceptible S. aureus (MSSA) isolates (68.8%, 99/144), MRSA isolates were more resistant to non-beta-lactam antimicrobials –trimethoprim/sulfamethoxazole 73.3% (33/45) vs. 27.3% (27/99) [p<0.0001]; erythromycin 75.6% (34/45) vs. 24.2% (24/99) [p<0.0001]; chloramphenicol 60% (27/45) vs. 19.2% (19/99) [p<0.0001]; gentamicin 55.6% (25/45) vs. 25.3% (25/99) [p=0.0004]; and ciprofloxacin 35.6% (16/45) vs. 2% (2/99) [p<0.0001]. Furthermore, 42 MRSA (93.3%) were multidrug resistant (MDR) and one exhibited high-level resistance to mupirocin. Overall, 61 MSSA (61.6%) were MDR, including three mupirocin and clindamycin resistant isolates. Seven spa types were detected among MRSA, of which t037 and t064 were predominant and associated with SCCmec types I and IV, respectively. Fourteen spa types were detected in MSSA which consisted mainly of t645 and t4353.

Conclusions:
S. aureus carriage rate in healthy children in Eastern Uganda is high and comparable to rates for hospitalized patients in Kampala. The detection of mupirocin resistance is worrying as it could rapidly increase if mupirocin is administered in a low-income setting. S. aureus strains of spa types t064, t037 (MRSA) and t645, t4353 (MSSA) are prevalent and could be responsible for majority of staphylococcal infections in Uganda.
Association Between Basilar Artery Configuration and Vessel Wall Features: A Prospective High-resolution Magnetic Resonance Imaging Study
Authors: Xu Ziqi , Li Mingyao , Hou Zhikai , Lyu Jinhao , Zhang Na , Lou Xin , Miao Zhongrong , Ma Ning 
Published: November 2019

Abstract
Background
The relationship between intracranial vessel configuration and wall features remains poorly investigated. Therefore, we aimed to investigate the relationship between the distal and proximal anatomical configuration of basilar artery (BA) and BA vessel wall features on high-resolution magnetic resonance imaging (HRMRI) .
Methods
From September 2014 to January 2017, patients with suspected symptomatic intracranial arterial stenosis underwent HRMRI. Patients with severe BA stenosis were selected for this prospective study and divided into two groups corresponding to complete and incomplete BA configuration based on characteristics of the bilateral vertebral arteries and posterior cerebral arteries. Culprit blood vessel wall features on HRMRI included plaque enhancement, intraplaque hemorrhage, remodeling patterns, and plaque distribution. Culprit vessel wall features were compared between patients in the complete and incomplete BA configuration groups.
Results
Among the 298 consecutively enrolled patients, 34 had severe BA stenosis. 20 patients had complete anatomical BA configuration and another 14 of them displayed incomplete configuration. There were no significant differences in vessel wall features between the complete and incomplete configuration patient groups. However, the proximal configuration of BA was associated with intraplaque hemorrhage (p=0.002) while the distal configuration of BA correlated with strong enhancement of BA plaque (p=0.041).
Conclusions
No association was found between the complete and incomplete BA configuration groups and blood vessel wall features. The proximal configuration of BA was related with intraplaque hemorrhage and the distal configuration of BA was associated with strong plaque enhancement. Further studies are warranted to confirm these findings.
Trial Registration : URL: http://www.clinicaltrials.gov. Unique identifier: NCT02705599 (March 10, 2016).
Validating a transnational fracture treatment registry using a standardized method
Authors: Frese Jasper , Gode Annalice , Heinrichs Gerhard , Will Armin , Schulz Arndt-Peter 
Published: November 2019

Abstract
Aim

Subsequent to a three-month pilot phase, recruiting patients for the newly established BFCC (Baltic Fracture Competence Centre) transnational fracture registry, a validation of the data quality needed to be carried out, applying a standardized method.Method

During the literature research, the method of “adaptive monitoring” fulfilled the requirements of the registry and was applied. It consisted of a three-step audit process; firstly, scoring of the overall data quality, followed by source data verification of a sample size, relative to the scoring result, and finally, feedback to the registry on measures to improve data quality. Statistical methods for scoring of data quality and visualisation of discrepancies between registry data and source data were developed and applied.Results

Initially, the data quality of the registry scored as medium. During source data verification, missing items in the registry, causing medium data quality, turned out to be absent in the source as well. A subsequent adaptation of the score evaluated the registry’s data quality as good. It was suggested to add variables to some items in order to improve the accuracy of the registry.Discussion

The application of the method of adaptive monitoring has only been published by Jacke et al., with a similar improvement of the scoring result following the audit process. Displaying data from the registry in graphs helped to find missing items and discover issues with data formats. Graphically comparing the degree of agreement between the registry and source data allowed to discover systematic faults.Conclusions

The method of adaptive monitoring gives a substantiated guideline for systematically evaluating and monitoring a registry’s data quality and is currently second to none. The resulting transparency of the registry’s data quality could be helpful in annual reports, as published by most major registries. As the method has been rarely applied, further successive applications in established registries would be desirable.
Validating a transnational fracture treatment registry using a standardized method
Authors: Frese Jasper , Gode Annalice , Heinrichs Gerhard , Will Armin , Schulz Arndt-Peter 
Published: November 2019

Abstract
Aim Subsequent to a three-month pilot phase, recruiting patients for the newly established BFCC (Baltic Fracture Competence Centre) transnational fracture registry, a validation of the data quality needed to be carried out, applying a standardized method.Method During the literature research, the method of “adaptive monitoring” fulfilled the requirements of the registry and was applied. It consisted of a three-step audit process; firstly, scoring of the overall data quality, followed by source data verification of a sample size, relative to the scoring result, and finally, feedback to the registry on measures to improve data quality. Statistical methods for scoring of data quality and visualisation of discrepancies between registry data and source data were developed and applied.Results Initially, the data quality of the registry scored as medium. During source data verification, missing items in the registry, causing medium data quality, turned out to be absent in the source as well. A subsequent adaptation of the score evaluated the registry’s data quality as good. It was suggested to add variables to some items in order to improve the accuracy of the registry.Discussion The application of the method of adaptive monitoring has only been published by Jacke et al., with a similar improvement of the scoring result following the audit process. Displaying data from the registry in graphs helped to find missing items and discover issues with data formats. Graphically comparing the degree of agreement between the registry and source data allowed to discover systematic faults.Conclusions The method of adaptive monitoring gives a substantiated guideline for systematically evaluating and monitoring a registry’s data quality and is currently second to none. The resulting transparency of the registry’s data quality could be helpful in annual reports, as published by most major registries. As the method has been rarely applied, further successive applications in established registries would be desirable.
The predictors and patterns of the early recurrence of pancreatic ductal adenocarcinoma after pancreatectomy: the influence of pre- and post- operative adjuvant therapy
Authors: Suto Hironobu , Okano Keiichi , Oshima Minoru , Ando Yasuhisa , Takahashi Shigeo , Shibata Toru , Kamada Hideki , Kobara Hideki , Masaki Tsutomu , Suzuki Yasuyuki 
Published: November 2019

Abstract
Background The perioperative factors predicting or influencing early pancreatic ductal adenocarcinoma recurrence are unclear. This study attempted to identify the predictive factors for early pancreatic ductal adenocarcinoma recurrence post-pancreatectomy and the influence of pre- and post- operative adjuvant therapy.
Methods One hundred and fifteen patients undergoing curative resection for pancreatic ductal adenocarcinoma between 2000 and 2016 at our institution were retrospectively analyzed. Patients were divided into two groups: those who did (n=34) and did not (n=81) experience a recurrence within 6 months postoperatively.
Results Multivariate analyses demonstrated postoperative CA19-9 de-normalization, no postoperative adjuvant chemotherapy, and serosal invasion were independent risk factors for early recurrence (P<0.001, P=0.001, and P=0.010, respectively). A subgroup analysis showed patients with (n=51) and without (n=64) preoperative chemoradiotherapy had different predictors. Although postoperative adjuvant chemotherapy was not a significant indicator in patients with preoperative chemoradiotherapy, CA19-9 de-normalization and no postoperative adjuvant chemotherapy were significant indicators in patients without preoperative chemotherapy. Preoperative chemotherapy strongly prevented early local recurrence while postoperative adjuvant chemotherapy prevented early distant recurrence.
Conclusions CA19-9 de-normalization was an important predictor of early recurrence of pancreatic ductal adenocarcinoma. Although postoperative adjuvant chemotherapy was an important preventive measure against early recurrence, particularly for distant recurrence, preoperative chemoradiotherapy could strongly prevent the early local recurrence of pancreatic ductal adenocarcinoma. These perioperative adjuvant therapies could have a complementary relationship.