bluuebunny/crossref_metadata_2025_split huggingface dataset:
Understanding barriers to women seeking and receiving help for perinatal mental health problems in UK general practice: development of a questionnaire
Authors: Ford Elizabeth , Roomi Hannah , Hugh Hannah , van Marwijk Harm 
Published: December 2019

AbstractAim:To develop a questionnaire to measure quantitatively barriers and facilitators to women’s disclosure of perinatal mental health problems in UK primary care. To pilot and evaluate the questionnaire for content validity and internal consistency.Background:Around 15% of women develop a mental illness in the perinatal period, such as depression, anxiety or post-traumatic stress disorder. In the United Kingdom, 90% of these women will be cared for in primary care, yet currently in as many as 50% of cases, no discussion of this issue takes place. One reason for this is that women experience barriers to disclosing symptoms of perinatal mental illness in primary care. These have previously been explored qualitatively, but no tool currently exists with which to measure these barriers quantitatively.Methods:Questionnaire items, drawn from qualitative literature and accounts of women’s experiences, were identified, refined iteratively and arranged in themes. The questionnaire was piloted using cognitive debriefing interviews to establish content validity. Women completed a refined version online. Responses were analysed using descriptive statistics. Internal consistency of subscales was calculated using Cronbach’s alpha.Findings:Cognitive debriefing interviews with five women showed the majority of questionnaire items were relevant, appropriate and easy to understand. The final questionnaire was completed by 71 women, and the majority of subscales had good internal consistency. The barrier scoring most highly was fear and stigma, followed by willingness to seek help and logistics of attending an appointment. Family/partner support and general practitioners’ (GPs) reaction were the lowest scoring barriers. Factors facilitating disclosure were GPs being empathetic and non-judgemental and listening during discussions. In the future, this questionnaire can be used to examine which barriers are most important for particular groups of women. This may enable the development of strategies to improve acknowledgement and discussion, and prevent under-recognition and under-treatment, of perinatal mental health problems in primary care.
Statistical Analysis ofTECDistributions Observed Over South and Central America
Authors: Villalobos J. , Valladares C. E. 
Published: December 2019

AbstractTotal electron content (TEC) values measured with several arrays of dual‐frequency GPS receivers operating continuously and simultaneously at equatorial and low latitudes are used to construct regional maps of TEC over South and Central America and the Caribbean region. This extended database comprises TEC values collected by 126 stations that operated in South America in 2008, 181 stations in 2009, and 324 stations in 2010. The latter year includes GPS stations in Central America and the Caribbean region, extending the TEC coverage from the southern tip of South America to the northern boundary of Mexico (~30°N latitude). The TEC maps contain high (0.5° × 0.5°) spatial resolution and good (30 min) temporal resolution. The most prominent feature of these maps is the day‐to‐day variability that is observed during all seasons and under quiet and active magnetic conditions. Single station plots display TEC variations with time scales of few hours and the appearance of TEC enhancements near‐midnight that can be accounted for by electric fields associated with disturbed magnetic conditions and the reverse fountain effect, respectively. To assess the TEC dependencies upon season, solar flux, magnetic activity, and local time over South and Central America, two statistical procedures were employed. First, we constructed monthly averages of TEC values along three field lines that intersect the magnetic equator at 70°, 60°, and 50°W longitudes that show a complex pattern of variability and symmetry/asymmetry of the equatorial anomaly depending on the season, the longitude, and the solar flux. Our second analysis consists of a non‐linear least‐square fit to simultaneously extract the solar flux, magnetic, and seasonal dependencies of TEC for each square cell (0.5° × 0.5°) of the regional maps and for each 30‐min time sector. It is found that during low solar flux conditions, the anomaly is weak or non‐existent. When the solar flux F10.7 index increases to 85 units the anomaly shows to be fully developed but contains a pronounced longitudinal/ seasonal dependence. During the equinoxes and afternoon hours, the northern crest extends across the South American continent, but the southern crest prevails in the eastern side of South America. It is found that the non‐linear numerical fitting of the TEC distributions can reproduce only 70% of the TEC variability.
The Southern Annular Mode and Southern Ocean Surface Westerly Winds in E3SM
Authors: Lee Doo Young , Petersen Mark R. , Lin Wuyin 
Published: December 2019

AbstractClimate variability and change in the Southern Hemisphere (SH) are influenced by the Southern Annular Mode (SAM) and are closely related to changes in the kinematic properties of the SH surface zonal winds. The SAM and SH surface zonal winds have strong effects on the atmospheric and oceanic circulation system. In this study we investigate the variability and trend in the SAM and position and strength of the surface zonal wind stress (TAUX), using two ensembles of simulations covering the historical record from the Energy Exascale Earth System Model (E3SM‐HIST and Atmospheric Model Intercomparison Project) for 1979–2014. In addition, performance of two CO
forcing simulations from the E3SM (E3SM‐1pctCO2 and 4xCO2) is assessed to examine the sensitivity of the variability and changes in the SAM and SH surface TAUX to climate forcing. In general, all E3SM simulations tend to capture the dominant feature of the SAM pattern reasonably well. The annual SAM index in the E3SM‐HIST simulation shows a significant increasing trend. These features are similar to the trends in the strength (along with poleward shift in the position) of the annual surface TAUX. For the climatological surface TAUX position and strength, the two CO
forcing simulations show slightly poleward movement and stronger intensity, while the E3SM‐HIST is equatorward and weaker than observations. In the relationship between the SAM and surface TAUX, we show that the SAM index exhibits a positive (negative) relationship with the strength (position) of the surface TAUX in the variability for all seasons and annual mean.
Quantified Aeolian Dune Changes on Mars Derived From Repeat Context Camera Images
Authors: Davis Joel M. , Grindrod Peter M. , Boazman Sarah J. , Vermeesch Pieter , Baird Thomas 
Published: December 2019

AbstractAeolian systems are active across much of the surface of Mars and quantifying the activity of bedforms is important for understanding the modern and recent Martian environment. Recently, the migration rates and sand fluxes of dunes and ripples have been precisely measured using repeat High Resolution Imaging Science Experiment (HiRISE) images. However, the limited areal extent of HiRISE coverage means that only a small area can be targeted for repeat coverage. Context Camera (CTX) images, although lower in spatial resolution, have wider spatial coverage, meaning that dune migration can potentially be monitored over larger areas. We used time series, coregistered CTX images and digital elevation models to measure dune migration rates and sand fluxes at six sites: Nili Patera, Meroe Patera, two sites at Herschel crater, McLaughlin crater, and Hellespontus Montes. We observed dune displacement in the CTX images over long‐term baselines (7.5–11 Earth years; 4–6 Mars years). Bedform activity has previously been measured at all these sites using HiRISE, which we used to validate our results. Our dune migration rates (0.2–1.1 m/EY) and sand fluxes (2.4–11.6 m3m−1EY−1) compare well to measurements made with HiRISE. The use of CTX in monitoring dune migration has advantages (wider spatial coverage, faster processing time) and disadvantages (ripples not resolved, digital elevation model dune heights may be underestimates); the future combined use of HiRISE and CTX is likely to be beneficial.
Scaling of Dust Flux With Friction Velocity: Time Resolution Effects
Authors: Dupont S. 
Published: December 2019

AbstractThe intermittency of aeolian soil erosion has motivated studies to extend to small time resolution (less than 15–30 min) the scaling property of erosion fluxes with the friction velocity
. However, common methods used to estimate
such as the law of the wall or the eddy covariance are only valid for stationary conditions, that is, long periods of 15–30 min. Unlike these methods, the wavelet transform method is applicable for nonstationary conditions. Here, this method is used to investigate the scaling property of the dust flux
with
at 1‐min and 10‐s resolutions, during erosion events. At 15‐min resolution,
and
estimated from the wavelet transform are identical to those obtained by eddy covariance. At smaller resolution,
and
exhibit strong fluctuations around their 15‐min trend, reflecting the nonstationarity of the flow and the intermittency of dust emission, respectively. While the 15‐min resolution
appears as correlated with
as with the mean wind speed
, with decreasing resolution,
becomes less correlated with
but still significantly correlated with
. Our results suggest that
is a suitable scaling parameter of
over usual 15‐ to 30‐min periods with the advantage of being height independent compared to
. However, at small time resolution,
becomes more relevant to scale
, the surface friction velocity becoming hardly accessible due to the absence of a constant momentum flux layer. Our study demonstrates the suitability of the wavelet transform to estimate dust fluxes at small time resolution or during nonstationary events.
Atmospheric Diffusivity: A New Energetic Framework for Understanding the Midlatitude Circulation Response to Climate Change
Authors: Mooring Todd A. , Shaw Tiffany A. 
Published: December 2019

AbstractThe midlatitude circulation response to climate change is not well understood. Within an energetic framework the circulation is connected to the atmospheric diffusivity. Here we define a novel diagnostic diffusivity framework as a tool for interpreting changes in the midlatitude atmospheric circulation. The diffusivity is computed by dividing the zonally and vertically integrated transient eddy moist static energy (MSE) transport by the zonal‐mean 925 hPa MSE gradient, and we investigate the diffusivity response to global‐scale sea surface temperature (SST) perturbations in aquaplanet and Atmospheric Model Intercomparison Project (AMIP) general circulation model simulations. The midlatitude diffusivity maximum shifts poleward with increasing SST in both the aquaplanet simulations and Southern Hemisphere winter of the AMIP simulations. Our framework indicates that this diffusivity shift is associated with Ferrel circulation and 925 hPa MSE gradient changes, rather than with total MSE transport changes. The MSE gradient‐related shift cannot be understood purely in terms of Clausius‐Clapeyron changes in specific humidity following the global‐mean temperature response, because atmospheric temperature gradients change significantly even when SST gradients are held fixed. The intensity of the diffusivity maximum also increases with SST for the aquaplanet configuration but is relatively insensitive to SST for AMIP Southern Hemisphere winter. Additional energy balance model experiments suggest that while diffusivity changes caused by SST perturbations can be large, their effect on zonal‐mean temperature is minor. Finally, the latitudes and intensities of the diffusivity maxima are strongly correlated with the same properties of the zonal‐mean eddy kinetic energy maxima. However, existing theories cannot adequately explain these linear relationships.
Swift evolutionary response of microbes to a rise in anthropogenic mercury in the Northern Hemisphere
Authors: Ruuskanen Matti O , Aris-Brosou Stéphane , Poulain Alexandre J 
Published: December 2019

Abstract
Anthropogenic mercury remobilization has considerably increased since the Industrial Revolution in the late 1700s. The Minamata Convention on Mercury is a United Nations treaty (2017) aiming at curbing mercury emissions. Unfortunately, evaluating the effectiveness of such a global treaty is hampered by our inability to determine the lag in aquatic ecosystem responses to a change in atmospheric mercury deposition. Whereas past metal concentrations are obtained from core samples, there are currently no means of tracking historical metal bioavailability or toxicity. Here, we recovered DNA from nine dated sediment cores collected in Canada and Finland, and reconstructed the past demographics of microbes carrying genes coding for the mercuric reductase (MerA)—an enzyme involved in Hg detoxification—using Bayesian relaxed molecular clocks. We found that the evolutionary dynamics of merA exhibited a dramatic increase in effective population size starting from 1783.8 ± 3.9 CE, which coincides with both the Industrial Revolution, and with independent measurements of atmospheric Hg concentrations. We show that even low levels of anthropogenic mercury affected the evolutionary trajectory of microbes in the Northern Hemisphere, and that microbial DNA encoding for detoxification determinants stored in environmental archives can be used to track historical pollutant toxicity.
Elastic kirigami patch for electromyographic analysis of the palm muscle during baseball pitching
Authors: Yamagishi Kento , Nakanishi Takenori , Mihara Sho , Azuma Masaru , Takeoka Shinji , Kanosue Kazuyuki , Nagami Tomoyuki , Fujie Toshinori 
Published: December 2019

Abstract
Surface electromyography (sEMG) is widely used to analyze human movements, including athletic performance. For baseball pitchers, a very precise movement is required to pitch the ball into the strike zone. The palm muscles appear to play a key role in this movement, and a real-time recording of sEMG from the palm muscle is useful in the analysis of motion during baseball pitching. However, the currently available devices with rigid and bulky electrodes (including connective wires) impede natural movements of the wearer and recording of sEMG from the palm muscles during vigorous action. Here, we describe a skin-contact patch consisting of kirigami-based stretchable wirings and conductive polymer nanosheet-based ultraconformable bioelectrodes, which address the challenge of mechanical mismatch between human skin and electrical devices. The key strategy is a kirigami-inspired wiring design and a mechanical gradient structure from nanosheet-based flexible bioelectrodes to a bulk wearable device. This approach would buffer the mechanical stress applied to the skin-contact bioelectrodes during an arm swing movement. With this patch, we precisely measure sEMG at the abductor pollicis brevis muscle (APBM) in a baseball player during ball pitching. We observe differences in the activity of the APBM between different types of pitches—fastball and curveball. This sEMG measurement system will enable the analysis of motion in unexplored muscle areas, such as on the palm and the sole, leading to a deeper understanding of muscular activity during performance in a wide range of sports and other movements.
Pan-cancer molecular subtypes revealed by mass-spectrometry-based proteomic characterization of more than 500 human cancers
Authors: Chen Fengju , Chandrashekar Darshan S. , Varambally Sooryanarayana , Creighton Chad J. 
Published: December 2019

AbstractMass-spectrometry-based proteomic profiling of human cancers has the potential for pan-cancer analyses to identify molecular subtypes and associated pathway features that might be otherwise missed using transcriptomics. Here, we classify 532 cancers, representing six tissue-based types (breast, colon, ovarian, renal, uterine), into ten proteome-based, pan-cancer subtypes that cut across tumor lineages. The proteome-based subtypes are observable in external cancer proteomic datasets surveyed. Gene signatures of oncogenic or metabolic pathways can further distinguish between the subtypes. Two distinct subtypes both involve the immune system, one associated with the adaptive immune response and T-cell activation, and the other associated with the humoral immune response. Two additional subtypes each involve the tumor stroma, one of these including the collagen VI interacting network. Three additional proteome-based subtypes—respectively involving proteins related to Golgi apparatus, hemoglobin complex, and endoplasmic reticulum—were not reflected in previous transcriptomics analyses. A data portal is available at UALCAN website.
TRPV1 activity and substance P release are required for corneal cold nociception
Authors: Li Fengxian , Yang Weishan , Jiang Haowu , Guo Changxiong , Huang Andrew J. W. , Hu Hongzhen , Liu Qin 
Published: December 2019

AbstractAs a protective mechanism, the cornea is sensitive to noxious stimuli. Here, we show that in mice, a high proportion of corneal TRPM8+ cold-sensing fibers express the heat-sensitive TRPV1 channel. Despite its insensitivity to cold, TRPV1 enhances membrane potential changes and electrical firing of TRPM8+ neurons in response to cold stimulation. This elevated neuronal excitability leads to augmented ocular cold nociception in mice. In a model of dry eye disease, the expression of TRPV1 in TRPM8+ cold-sensing fibers is increased, and results in severe cold allodynia. Overexpression of TRPV1 in TRPM8+ sensory neurons leads to cold allodynia in both corneal and non-corneal tissues without affecting their thermal sensitivity. TRPV1-dependent neuronal sensitization facilitates the release of the neuropeptide substance P from TRPM8+ cold-sensing neurons to signal nociception in response to cold. Our study identifies a mechanism underlying corneal cold nociception and suggests a potential target for the treatment of ocular pain.
Maternal vaccination and protective immunity against Zika virus vertical transmission
Authors: Shan Chao , Xie Xuping , Luo Huanle , Muruato Antonio E. , Liu Yang , Wakamiya Maki , La Jun-Ho , Chung Jin Mo , Weaver Scott C. , Wang Tian , Shi Pei-Yong 
Published: December 2019

AbstractAn important goal of the Zika virus (ZIKV) vaccine is to prevent a congenital syndrome in fetuses of pregnant women, but studies directly evaluating maternal vaccination for ZIKV are lacking. Here we report maternal vaccination using a live-attenuated ZIKV vaccine (3ʹUTR-∆10-LAV) in a pregnant mouse model. Maternal immunization with 3ʹUTR-∆10-LAV does not cause any adverse effects on pregnancy, fetal development, or offspring behavior. One maternal immunization fully protects dams against ZIKV infection and in utero transmission. Although neutralizing antibody alone is sufficient to prevent in utero transmission, a higher neutralizing titer is required to protect pregnant mice against in utero transmission than that required to protect non-pregnant mice against viral infection. The immunized dams transfer maternal antibodies to pups, which protect neonates against ZIKV infection. Notably, pregnancy weakens maternal T cell response to 3ʹUTR-∆10-LAV vaccination. Our results suggest that, besides vaccinating non-pregnant individuals, 3ʹUTR-∆10-LAV may also be considered for maternal vaccination.
A metal–peptide capsule by multiple ring threading
Authors: Sawada Tomohisa , Inomata Yuuki , Shimokawa Koya , Fujita Makoto 
Published: December 2019

AbstractCavity creation is a key to the origin of biological functions. Small cavities such as enzyme pockets are created simply through liner peptide folding. Nature can create much larger cavities by threading and entangling large peptide rings, as learned from gigantic virus capsids, where not only chemical structures but the topology of threaded rings must be controlled. Although interlocked molecules are a topic of current interest, they have for decades been explored merely as elements of molecular machines, or as a synthetic challenge. No research has specifically targeted them for, and succesfully achieved, cavity creation. Here we report the emergence of a huge capsular framework via multiple threading of metal–peptide rings. Six equivalent C4-propeller-shaped rings, each consisting of four oligopeptides and Ag+, are threaded by each other a total of twelve times (crossing number: 24) to assemble into a well-defined 4 nm-sized sphere, which acts as a huge molecular capsule.
Audio-visual experience strengthens multisensory assemblies in adult mouse visual cortex
Authors: Knöpfel Thomas , Sweeney Yann , Radulescu Carola I. , Zabouri Nawal , Doostdar Nazanin , Clopath Claudia , Barnes Samuel J. 
Published: December 2019

AbstractWe experience the world through multiple senses simultaneously. To better understand mechanisms of multisensory processing we ask whether inputs from two senses (auditory and visual) can interact and drive plasticity in neural-circuits of the primary visual cortex (V1). Using genetically-encoded voltage and calcium indicators, we find coincident audio-visual experience modifies both the supra and subthreshold response properties of neurons in L2/3 of mouse V1. Specifically, we find that after audio-visual pairing, a subset of multimodal neurons develops enhanced auditory responses to the paired auditory stimulus. This cross-modal plasticity persists over days and is reflected in the strengthening of small functional networks of L2/3 neurons. We find V1 processes coincident auditory and visual events by strengthening functional associations between feature specific assemblies of multimodal neurons during bouts of sensory driven co-activity, leaving a trace of multisensory experience in the cortical network.
Evolution of imprinting via lineage-specific insertion of retroviral promoters
Authors: Bogutz Aaron B. , Brind’Amour Julie , Kobayashi Hisato , Jensen Kristoffer N. , Nakabayashi Kazuhiko , Imai Hiroo , Lorincz Matthew C. , Lefebvre Louis 
Published: December 2019

AbstractImprinted genes are expressed from a single parental allele, with the other allele often silenced by DNA methylation (DNAme) established in the germline. While species-specific imprinted orthologues have been documented, the molecular mechanisms underlying the evolutionary switch from biallelic to imprinted expression are unknown. During mouse oogenesis, gametic differentially methylated regions (gDMRs) acquire DNAme in a transcription-guided manner. Here we show that oocyte transcription initiating in lineage-specific endogenous retroviruses (ERVs) is likely responsible for DNAme establishment at 4/6 mouse-specific and 17/110 human-specific imprinted gDMRs. The latter are divided into Catarrhini- or Hominoidea-specific gDMRs embedded within transcripts initiating in ERVs specific to these primate lineages. Strikingly, imprinting of the maternally methylated genes Impact and Slc38a4 was lost in the offspring of female mice harboring deletions of the relevant murine-specific ERVs upstream of these genes. Our work reveals an evolutionary mechanism whereby maternally silenced genes arise from biallelically expressed progenitors.