bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prevalence of Short Stature and Malnutrition among Egyptian Primary School Children and their Coexistence with Anemia
Authors: Shafie Ali M. El , Kasemy Zeinab A. , omar Zein A. , Alkalash Safa H. , salama Amal A. , mahrous kerollos s. , Hewedy Shaimaa M. , Kotb Nessreen M. , El-Hady Heba S. Abd , Eladawy Eman S. , Zeid Mohamed A. , Hamid Manar E. Abd El , Hemeda Emad H. , Elshafie Mohamed A. , meligy Esraa A. El , bahbah Wael A. 
Published: May 2020

Abstract
Background: Under nutrition and overweight typically occur during nutritional transition periods in developing countries including Egypt. Short stature and anemia are public health concern due to its strong link with malnutrition which is a preventable risk factor. Objectives: to estimate the prevalence of overweight, obesity, underweight and short stature and its concurrence with anemia, also to determine the etiological profile of short stature among primary school children in Egypt.Methods: A cross-sectional study was carried out on 33150 Egyptian children aged 6-11 years old from January 2018 to January 2020, allocated in 59 primary schools from diverse geographical districts in Egypt. Complete anthropometric measurements were conducted and applied according to WHO growth charts. Hemoglobin level was measured. Systematic approach to detect the etiology of short stature was applied randomly to a sample of 380 stunted children.Results: The prevalence of underweight was 8.2 %, while obesity and overweight represented 21.8% (9.6% and 12.2% respectively). Overall short stature constituted 17%. The main etiologies of short stature were familial (40.8%) and constitutional (24.2%). Anemia was diagnosed in 26% of children; while concurrent anemia and stunting was reported in 9.9%. Regarding anemia and anemia with stunting were more common among girls (30.0 % (OR=1.50, CI95%: 1.43-1.58) and 11.4% (OR=1.39, CI95%:1.29-1.49) respectively), who were living in rural areas (33.4% (OR=1.96, CI 95%:1.87-2.06) &12.7% (OR= 1.72, CI 95%:1.60-1.85)) and those who had low socioeconomic status )34.6% (OR= 2.54, CI 95%:2.29-2.82) & 17.2% (OR= 3.32, CI 95%:2.85-3.88() respectively. Anemia with stunting was significantly higher among children aged ≥9 years old representing 12% (OR= 1.40, CI 95%:1.30-1.51). Conclusion: Prevalence of short stature, obesity and anemia was high among primary school children in Egypt with a strong concurrence between anemia and stunting. Intensive parental health education and in-depth nutritional assessment are required.
SUV39H1-mediated DNMT3A is participated in the epigenetic regulation of Tim-3 and galectin-9 in cervical cancer
Authors: Zhang Li , Tian Sijuan , Zhao Minyi , Yang Ting , Quan Shimin , Yang Qing , Song Lihua , Yang Xiaofeng 
Published: May 2020

Abstract
Background: Methylation of histone 3 at lysine 9 (H3K9) and DNA methylation are among the most highly conserved epigenetic marks that correlate well with gene silencing. The tumor microenvironment significantly influences therapeutic responses and clinical outcomes. The epigenetic-regulation mechanism of the costimulatory factors Tim-3 and galectin-9 in cervical cancer remains unknown. Methods: The methylation status of HAVCR2 and LGALS9 was detected by MS-PCR in cervical cancer tissues and cell lines. The underlying molecular mechanisms of SUV39H1-DNMT3A-Tim-3/galectin-9 regulation was elucidated using cervical cancer cell lines containing siRNA or/and over-expression system. Confirmation of the regulation of DNMT3A by SUV39H1 used ChIP-qPCR. Results: Here, we show that SUV39H1 up-regulates H3K9me3 expression in DNMT3A promoter region, which in turn induced expression of DNMT3A. In addition, our mechanistic studies indicate that DNMT3A mediates the epigenetic modulation of the HAVCR2 and LGALS9 genes by directly binding to their promoter regions in vitro . Moreover, in an in vivo assay, the expression profile of SUV39H1 up-regulates the level of H3K9me3 in the DNMT3A promoter region was found to correlate with Tim-3 and galectin-9 expression at the cellular level，indicating that SUV39H1-H3K9me3-DNMT3A is a crucial regulatory axis in cervical cancer. Conclusion: These results indicate that SUV39H1-DNMT3A is a crucial Tim-3 and galectin-9 regulatory axis in cervical cancer.
Identification of Differential Methylation Regions and Correspondence Targeted Genes in Oral Squamous Cell Carcinoma
Authors: Niu Yan , Shan Qiusheng , Guo Yuanling 
Published: May 2020

Abstract
Background

The differential methylation included hypermethylation and hypomethylation plays significant role in the progression of many kind of cancers but study little in oral squamous cell carcinoma (OSCC).
Methods

GSE123781 and GSE87053 was used to analysis the differential methylation regions (DMRs) and predict the target genes in OSCC by R software and wANNOVAR respectively. the biological process and cell pathways of common targeted genes between GSE123781 and GSE87053 were analyzed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes pathway (KEGG) enrichment analysis. The hub genes (hub genes 1) in common targeted genes associated with cancer biological process was identified by protein to protein interaction network (PPI). In addition, GSE74530 and GSE30784 was used to identify common differentially expressed genes (DEGs) in OSCC by R software. The biological process and cell pathways of common DEGs was analyzed by GO and KEGG enrichment analysis. The hub genes (hub genes 2) in common DEGs associated with cancer biological process was identified by PPI network. The significant hub genes between hub genes 1 and hub genes 2 were identified by Venn picture. Finally, the expression level of significant hub genes and correspondence relationship with head and neck squamous cell carcinoma (HNSCC) patient survival were confirmed by The Cancer Genome Atlas (TCGA) dataset.
Results

There are 2146 common targeted genes regulated by DMRs between GSE123781 and GSE87053 and 278 hub genes in common targeted genes associated with cancer biological process. In addition, there are 895 common DEGs between GSE74530 and GSE30784 and 144 hub genes in common DEGs associated with cancer biological process. There are 9 significant hub genes between hub genes 1 and hub genes 2. Finally, these 9 significant hub genes differentially expressed in HNSCC tissues except CCR7 and quite associated with the survival of HNSCC patients.
Conclusions

CCR7, ETS1, RUNX3, CCR1, C3AR1, LAMB1, IRF7, LGALS3 and CDKN3 both are DEGs and regulated by DMRs in OSCC, which are quite associated with the progression of OSCC and the survival of HNSCC patients. All of these genes have much potential to be new biomarkers in targeted therapy of OSCC.
Comparison of Overall Survival of Gastric Neoplasms Containing Neuroendocrine Carcinoma Components with Gastric Adenocarcinoma: A Propensity Score Matching Study
Authors: Chen Jiahui , Wang Anqiang , Ji Ke , Bu Zhaode , Ji Jiafu 
Published: May 2020

Abstract
Background Gastric neoplasms containing neuroendocrine carcinoma (NEC) components are rare malignancies with highly aggressive behavior and poor prognosis, including pure NEC and mixed tumor containing NEC components. We attempt to investigate whether there is a distinct difference in overall survival (OS) between Gastric neoplasms containing NEC components and gastric adenocarcinoma.Methods Surgically resected gastric neoplasms containing NEC components (n = 54) and gastric adenocarcinoma (n = 600) from January 2013 to December 2019 at Peking University Cancer Hospital were retrospectively analyzed. Patients were categorized into the surgical group and the neoadjuvant group and adjusted using propensity score matching. In the two groups, gastric neoplasms containing NEC components were divided into pure NEC, mixed tumors with neuroendocrine carcinoma components less than 30% (< 30% G-HMiNEN), between 30% and 70% (G-HMiNEN) and more than 70% (> 70% G-HMiNEN). OS was compared between these groups and gastric adenocarcinoma.Results The OS of gastric neoplasms containing neuroendocrine NEC components was poorer than that of gastric adenocarcinoma in the surgical group, whether the ratio of neuroendocrine cancer components was less than 30%, between 30% and 70%, higher than 70% or 100%. Cox multivariate regression analysis proved tumor category (neoplasms containing NEC components or gastric adenocarcinoma) to be an independent risk factor for prognosis. In patients receiving neoadjuvant therapy, however, the difference was not significant.Conclusions Gastric neoplasms containing NEC components, regardless of the proportion of neuroendocrine carcinoma components, had poorer overall survival than gastric adenocarcinoma in patients treated with surgery directly, indicating a higher degree of malignancy than gastric adenocarcinoma.
Serum CGRP, VIP, and PACAP Usefulness in Migraine: A Case-Control Study in Chronic Migraine Patients in Real Clinical Practice.
Authors: Pereda Sara Pérez , Toriello-Suárez María , Ocejo-Vinyals Gonzalo , Guiral-Foz Sandra , Castillo-Obeso Jesús , Montes-Gómez Silvia , Martínez-Nieto Rosa M , Iglesias Fernando , González-Quintanilla Vicente , Oterino Agustin 
Published: May 2020

Abstract
Background: Calcitonin gene-related peptide (CGRP), vasoactive intestinal peptide (VIP) and pituitary adenylate cyclase activating polypetide-38 (PACAP-38) have relevant roles in migraine pathophysiology. Their serum levels have been proposed as biomarkers for migraine. Our aim was to assess their diagnostic value in real clinical practice in a cohort of chronic migraine (CM), episodic migraine (EM) and healthy controls (HC).Methods: We recruited subjects with CM, EM and HC at two medical centers. Blood samples were drawn under fasting conditions in the interictal period, immediately centrifuged and stored at -80º C. Serum levels were determined by ELISA. Neuropeptide levels, the effect of preventatives, correlations with clinical and demographic variables, and their diagnostic value were studied among clinical categories.Results: 296 age- and sex-matched subjects (101 CM, 98 EM and 97 HC) were included. All three neuropeptide serum levels were higher in CM [median and IQ for CGRP= 18.023 pg/ml (14.4-24.7); VIP= 121.732 pg/ml (48.72-186.72) and PACAP= 204.931 pg/ml (101.08-597.64)] vs EM [CGRP = 14.659 pg/ml (10.29-17.45); VIP = 75.603 pg/ml (28.722-107.10); and PACAP = 94.992 pg/ml (65.77-128.48)] and vs HC [CGRP = 13.988 pg/ml (10.095-17.87); VIP = 84.685 pg/ml (35.32-99.79), and PACAP = 103.142 pg/ml (59.42-123.97)]. Using multinomial modeling, only VIP (OR 1.011, 95% CI=1.003-1.018, p=0.005) and PACAP (OR=1.003, 95% CI=1.001-1.005, p=0.002) increased the risk for CM, but not for EM. CGRP did not predict CM or EM. This model could correctly classify only 62/101 (61.38%) of CM, 75/98 (76.53%) of EM, and 5/97 (4.12%) of HC [globally 147/296 (49.8%)]. Individually, PACAP performed the best for classifying clinical categories [global accuracy 150/296 (50.67%)]. In CM, neuropeptide levels were higher in those OnaBT-treated than in no-treated patients.Conclusions: Although interictal serum CGRP and VIP were higher in CM than both EM or HC, their utility to discriminate migraine categories was low. Contrary to other studies, PACAP serum levels were also higher in CM than in EM or HC and had more discriminative capability to distinguish CM from EM and HC. Further investigation is needed for determination technique standardization.
Prevalence and impact of burnout, secondary traumatic stress and compassion satisfaction on hand hygiene of healthcare workers in medical aid team during COVID-19 pandemic
Authors: Zhou Qian , Lai Xiaoquan , Wan Chaoyang , Zhang Xinping , Tan Li 
Published: May 2020

Abstract
Background Healthcare workers suffered mental burden, especially in the period of COVID-19. Professional quality of life quality is suitable to measure how healthcare workers feel in medical aid team. Current evidence of impact of professional quality of life on hand hygiene behavior or even IPC measures was limited, especially in emerging infectious disease period. This study aimed to assess the prevalence of burnout, secondary traumatic stress and compassion satisfaction and explore their impact on self-reported hand hygiene behavior among medical aid team in Wuhan, China, where strict management was conducted to prevent COVID-19 spread and guarantee healthcare workers’ health. Results A cross-sectional study was conducted using online questionnaire covering professional quality of life and self-reported hand hygiene behavior based on COVID-19 guideline. A total of 1,734 healthcare workers were surveyed. The prevalence of burnout, secondary trauma and compassion satisfaction were low and average levels (69.61 and 30.39%), low and average levels (33.33 and 66.21%), average and high levels (49.65 and 49.71%), respectively. Burnout was negatively associated with overall hand hygiene (Coef. =-0.088, p<0.001), low hand hygiene (Coef. =-0.109, p<0.001), medium hand hygiene (Coef. =-0.088, p<0.001) and high hand hygiene (Coef. =-0.065, p<0.001). Conclusions Healthcare workers with higher compassion satisfaction reported higher hand hygiene compared to the lower. Healthcare workers in medical aid team experience lower level burnout, and higher level of compassion satisfaction during COVID-19 pandemic compared to the general period. The lower burnout and higher compassion satisfaction are associated with higher self-reported hand hygiene behavior. Burnout and compassion satisfaction in healthcare workers should be emphasize and need interventions targeting. The management of healthcare workers in Wuhan, China may be constructive for the future medical aid team.
Ultrasound-guided core needle biopsy compared to open biopsy: a new diagnostic approach to major salivary gland enlargement in Sjögren’s syndrome
Authors: Zabotti Alen , Callegher Sara Zandonella , Lorenzon Michele , Pegolo Enrico , Scott Cathryn Anne , Tel Alessandro , Giovannini Ivan , Robiony Massimo , Loreto Carla Di , Zuiani Chiara , Vita Salvatore De 
Published: May 2020

Abstract
Objective

Persistent (≥2 months) major salivary gland (SG) enlargement in primary Sjögren's syndrome (pSS) patients is a well-known sign of possible involvement by B-cell lymphoma. The study aimed to evaluate the diagnostic accuracy and safety of ultrasound-guided core needle biopsy (US-guided CNB) of major SGs in comparison to open surgical biopsy.

Methods

Prospective pSS patients (cases) with clinically persistent SG enlargement underwent US-guided CNB, and were compared to retrospective pSS patients (controls) submitted to open surgical biopsy. The features analyzed were pre-biopsy clinical and laboratory findings, biopsy-related complications (reported by the patient with a questionnaire and clinically verified), adequacy of the material for histology and diagnosis rendered.

Results

Thirteen cases underwent US-guided CNB: in 9/13 biopsy was performed on the parotid and in 4/13 on the submandibular gland. Sufficient material was obtained for pathological diagnosis in all samples. The final diagnoses were: 5/13 (38.5%) B-cell lymphoma, 1/13 (7.7%) lympho-epithelial sialadenitis, 4/13 (30.7%) other sialadenitis (granulomatous consistent with sarcoidosis, IgG4-related disease, chronic sclerosing, diffuse chronic) and 3/13 (23.1%) miscellaneous lesions. Thirteen controls underwent open surgical biopsy of the parotid, in one inadequate material was obtained, in 12 (92.3%) the pathologic diagnoses were: 4/12 (33.3%) B-cell lymphoma, 2/12 (16.7%) lympho-epithelial sialadenitis, 4/12 (33.3%) uncertain lymphoproliferative lesions, 2/12 (16.7%) miscellaneous lesions. 6/13 (46.1%) cases reported 6 transient complications, and 12/13 (92.3%) controls 2 persistent and 14 transient complications.

Conclusion

US-guided CNB represents a novel, clinically relevant and safe approach to the management of pSS patients with parotid or submandibular persistent enlargement.
Imaging analysis of 13 rare cases of renal collecting (Bellini) duct carcinoma patients in northen China.
Authors: Lyu Zhehao , Liu Lili , Li Huimin , Wang Haibo , Liu Qi , Chen Tingting , Xu Meiling , Tian Lin , Fu Peng 
Published: May 2020

Abstract
Background: Collective duct carcinoma (CDC) is a highly malignant kidney tumor which is rare in clinical. We report our 12-year experience of Collecting (Bellini) duct carcinoma (CDC) and retrospectively analyzed patients and tumour characteristics, clinical manifestations, different imaging characteristics including CT, MRI and PET/CT. Methods: From January 2007 to December 2019, we retrospectively examined all renal tumors and identifed 13 cases of CDC and tried to improve the understanding of the imaging features of the disease.Results: The study group included 10 men and 3 women, with an average age of 64.23±10.74 years old. Of these 13 patients, the main clinical manifestations include gross hematuria, flank pain or waist discomfort. The mean tumour size was 8.48 ± 2.48 cm. In this group of cases, 6 cases are cortical-medullary involved type, 7 cases are cortex-medullary-pelvis involved type. 11 cases were nephritis type and 2 cases were mass type. The lesions appeared solid or complex solid and cystic on CT and MRI. The parenchymal part of 13 CDC tumors showed isodensity or slightly higher density on unenhanced CT scan. Two patients with PET/CT showed increased radioactivity intake. Evidence of intra-abdominal metastatic disease was present on CT in 9 of 13 ( 69.23% ) cases. The incidence of metastasis reached 69%.Conclusions: The Collecting (Bellini) duct carcinoma has its certain imaging characteristics which were different from the other renal cell carcinoma. A renal tumor should be considered as CDC when it locates in the junction zone of the renal cortex and medulla, with unclear border, slight enhancement and metastases in early stage. PET/CT can greatly enrich the key information of diagnosis, surgery and treatment options, which provide significant help to the clinic.
Nuclear overexpression levels of MAGE-A3 predict poor prognosis in patients with prostate cancer
Authors: khalvandi Azadeh , Abolhasani Maryam , Madjd Zahra , Shekarabi Mehdi , Arami Masoumeh Kourosh , Mohsenzadegan Monireh 
Published: May 2020

Abstract
Melanoma antigen gene A3 (MAGE-A3) is one of the most immunogenic cancer- testis antigens and is common in various types of cancers. Due to its high immunogenicity and oncogenicity, it has been a candidate for cancer immunotherapy and used as a cancer vaccine in clinical trials of various types of cancers. In this study, for the first time, we performed immunohistochemical analysis to evaluate the expression of MAGE-A3 in 153 formalin-fixed paraffin-embedded prostate tissue samples including 112 cases of prostate cancer (PCa), 22 cases of benign prostatic hyperplasia (BPH) and 19 cases of high-grade prostatic intraepithelial neoplasia (HPIN). Both nuclear and cytoplasmic expressions of MAGE-A3 with different staining intensities were observed among the tissues. Increased expression of MAGE-A3 was significantly found in PCa tissues compared to both HPIN and BPH tissues (nuclear expression at P = 0.011, and cytoplasmic expression at p = 0.034; for both comparisons P < 0.0001, respectively). A significant correlation was observed between higher nuclear and cytoplasmic expressions of MAGE-A3 with Gleason score (P < 0.0001 and 0.006, respectively). Increased expression of MAGE-A3 was associated with shorter biochemical recurrence-free survival (BCR-FS) and disease-free survival (DFS) of patients (P = 0.042 and P = 0.0001, respectively). In multivariate analysis, nuclear expression of MAGE-A3 and Gleason score (≤ 7 vs > 7) were independent predictors of the DFS (both; P = 0.019). Nuclear expression of MAGE-A3 was also significantly related to BCR-FS (P = 0.015). MAGE-A3 can be considered as a valuable predictor for poor prognosis and an attractive option for vaccine immunotherapy in patients with PCa.
Persistent Barriers to the Adoption of Active Surveillance in Low-Risk Prostate Cancer: A Qualitative Analysis of Community and Academic Urologists
Authors: Ellis Shellie D , Hwang Soohyun , Morrow Emily , Kimminau Kim S. , Goonan Kelly , Petty Laurie , Ellerbeck Edward , Thrasher J Brantley 
Published: May 2020

Abstract
Background Clinical practice guidelines recommend active surveillance as the preferred treatment option for low-risk prostate cancer, but only a minority of eligible men receive active surveillance, and practice variation is substantial. The aim of this study is to describe barriers to urologists’ recommendation of active surveillance in low-risk prostate cancer and explore variation of barriers by setting.Methods We conducted semi-structured interviews among 22 practicing urologists, evenly distributed between academic and community practice. We coded barriers to active surveillance according to a conceptual model of determinants of treatment quality to identify potential opportunities for intervention.Results Community and academic urologists were generally in agreement on factors influencing active surveillance. Urologists perceived patient-level factors to have the greatest influence on recommendations, particularly tumor pathology, patient age, and judgements about the patient’s ability to adhere to follow-up protocols. They also noted cross-cutting clinical barriers, including concerns about the adequacy of biopsy samples, inconsistent protocols to guide active surveillance, and side effects of biopsy procedures. Urologists had differing opinions on the impact of environmental factors, such as financial disincentives and fear of litigation.Conclusions Despite national and international recommendations, both academic and community urologists note a variety of barriers to implementing active surveillance in low risk prostate cancer. These barriers will need to be specifically addressed in efforts to help urologists offer active surveillance more consistently.Trial Registration: N/A