bluuebunny/crossref_metadata_2025_split huggingface dataset:
Long non-coding RNA, LINC01614 as a potential biomarker for prognostic prediction in breast cancer
Authors: Wang Yaozong , Song Baorong , Zhu Leilei , Zhang Xia 
Published: November 2019

Background
Dysregulated long non-coding RNAs (lncRNAs) may serve as potential biomarkers of cancers including breast cancer (BRCA). This study aimed to identify lncRNAs with strong prognostic value for BRCA.

Methods
LncRNA expression profiles of 929 tissue samples were downloaded from TANRIC database. We performed differential expression analysis between paired BRCA and adjacent normal tissues. Survival analysis was used to identify lncRNAs with prognostic value. Univariate and multivariate Cox regression analyses were performed to confirm the independent prognostic value of potential lncRNAs. Dysregulated signaling pathways associated with lncRNA expression were evaluated using gene set enrichment analysis.

Results
We found that a total of 398 lncRNAs were significantly differentially expressed between BRCA and adjacent normal tissues (adjusted P value <= 0.0001 and |logFC| >= 1). Additionally, 381 potential lncRNAs were correlated Overall Survival (OS) (P value < 0.05). A total of 48 lncRNAs remained when differentially expressed lncRNAs overlapped with lncRNAs that had prognostic value. Among the 48 lncRNAs, one lncRNA (LINC01614) had stronger prognostic value and was highly expressed in BRCA tissues. LINC01614 expression was validated as an independent prognostic factor using univariate and multivariate analyses. Higher LINC01614 expression was observed in several molecular subgroups including estrogen receptors+, progesterone receptors+ and human epidermal growth factor receptor 2 (HER2)+ subgroup, respectively. Also, BRCA carrying one of four gene mutations had higher expression of LINC01614 including AOAH, CIT, HER2 and ODZ1. Higher expression of LINC01614 was positively correlated with several gene sets including TGF-β1 response, CDH1 signals and cell adhesion pathways.

Conclusions
A novel lncRNA LINC01614 was identified as a potential biomarker for prognosis prediction of BRCA. This study emphasized the importance of LINC01614 and further research should be focused on it.
Genetic and phylogenetic analysis of Chinese sacbrood virus isolates fromApis mellifera
Authors: Li Ming , Fei Dongliang , Sun Li , Ma Mingxiao 
Published: November 2019

BackgroundSacbrood virus (SBV) is one of the most pathogenic honeybee viruses that exhibits host specificity and regional variations. The SBV strains that infect the Chinese honeybeeApis ceranaare called Chinese SBVs (CSBVs).MethodsIn this study, a CSBV strain named AmCSBV-SDLY-2016 (GenBank accession No.MG733283) infectingA. melliferawas identified by electron microscopy, its protein composition was analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis and agar gel immunodiffusion assay, and its nucleotide sequence was identified using a series of reverse-transcription polymerase chain reaction fragments of AmCSBV-SDLY-2016 generated using SBV/CSBV-specific primers. To investigate phylogenetic relationships of the CSBV isolates, a phylogenetic tree of the complete open reading frames (ORF) of the CSBV sequences was constructed using MEGA 6.0; then, the similarity and recombination events among the isolated CSBV strains were analyzed using SimPlot and RDP4 software, respectively.ResultsSequencing results revealed the complete 8,794-nucleotide long complete genomic RNA of the strain, with a single large ORF (189–8,717) encoding 2,843 amino acids. Comparison of the deduced amino acid sequence with the SBV/CSBV reference sequences deposited in the GenBank database identified helicase, protease, and RNA-dependent RNA polymerase domains; the structural genes were located at the 5′ end, whereas the non-structural genes were found at the 3′ end. Multiple sequence alignment showed that AmCSBV-SDLY-2016 had a 17-amino acid (aa) and a single aa deletion at positions 711–729 and 2,128, respectively, as compared with CSBV-GD-2002, and a 16-aa deletion (positions 711–713 and 715–728) as compared with AmSBV-UK-2000. However, AmCSBV-SDLY-2016 was similar to the CSBV-JLCBS-2014 strain, which infectsA. cerana. AmCSBV-SDLY-2016 ORF shared 92.4–97.1% identity with the genomes of other CSBV strains (94.5–97.7% identity for deduced amino acids). AmCSBV-SDLY-2016 was least similar (89.5–90.4% identity) to other SBVs but showed maximum similarity with the previously reported CSBV-FZ-2014 strain. The phylogenetic tree constructed from AmCSBV-SDLY-2016 and 43 previously reported SBV/CSBV sequences indicated that SBV/CSBV strains clustered according to the host species and country of origin; AmCSBV-SDLY-2016 clustered with other previously reported Chinese and Asian strains (AC genotype SBV, as these strains originated fromA. cerana) but was separate from the SBV genomes originating from Europe (AM genotype SBV, originating fromA. mellifera). A SimPlot graph of SBV genomes confirmed the high variability, especially between the AC genotype SBV and AM genotype SBV. This genomic diversity may reflect the adaptation of SBV to specific hosts, ability of CSBV to cross the species barrier, and the spatial distances that separate CSBVs from other SBVs.
Haematopoietic stem cell reprogramming and the hope for a universal blood product
Authors: Barcia Durán José Gabriel , Lis Raphaël , Rafii Shahin 
Published: November 2019

Haematopoietic stem cells (HSCs) are the only adult stem cells with a demonstrated clinical use, even though a tractable method to maintain and expand human HSCs in vitro has not yet been found. Owing to the introduction of transplantation strategies for the treatment of haematological malignancies and, more recently, the promise of gene therapy, the need to improve the generation, manipulation and scalability of autologous or allogeneic HSCs has risen steeply over the past decade. In that context, reprogramming strategies based on the expression of exogenous transcription factors have emerged as a means to produce functional HSCs in vitro. These approaches largely stem from the assumption that key master transcription factors direct the expression of downstream target genes thereby triggering haematopoiesis. Both somatic and pluripotent cells have been used to this end, yielding variable results in terms of haematopoietic phenotype and functionality. Here, we present an overview of the haematopoietic reprogramming methods reported to date, provide the appropriate historical context and offer some critical insight about where the field stands at present.
Monitoring the Real‐Time Circulatory System‐Related Physiological and Pathological Processes In Vivo Using a Multifunctional NIR‐II Probe
Authors: Li Daifeng , Qu Chunrong , Liu Qiang , Wu Yifan , Hu Xiang , Qian Kun , Chang Baisong , He Shuqing , Yuan Ying , Li Yizhou , Ko Timothy , Yu Aixi , Cheng Zhen 
Published: November 2019

AbstractThe dysfunction of the circulatory system leads to various pathological processes with high morbidity. Recently, fluorescence imaging in the near‐infrared II (NIR‐II) window (1000–1700 nm) has attracted immense attention in many biological processes. The rapid metabolism and low toxicity of some NIR‐II organic small molecules indicate their feasibility for use in visualizing the circulatory system. However, most of the reported NIR‐II organic small molecules presently encounter such dilemmas as complicated synthetic procedures and low quantum yields (QY). To address this challenge, a series of facilely prepared NIR‐II organic small molecule CQ‐T (CQ‐1‐4T) are designed and these compounds are loaded with biocompatible human serum albumin (HSA) to improve QY. Among them, CQL (CQ‐4T/HSA) demonstrates superior optical properties and a 6.65‐fold increase in fluorescence compared to the small molecule alone. Further work validates the efficacy and accuracy of CQL in monitoring the real‐time circulatory system‐related physiological and pathological processes in vivo, including thrombosis, peripheral arterial disease, tumor angiogenesis, and lymphatic drainage. Moreover, the excellent optical properties of CQL enable precise tumor resection and sentinel lymph node biopsy under NIR‐II navigation. In conclusion, CQL is a novel and promising NIR‐II organic probe with multifunctional imaging capability. It is highly desirable to accelerate its future translations into the clinic.
Flexible Integrated Sensors: Transverse Piezoresistance and Longitudinal Thermal Resistance of One Single Carbon Fiber Beam
Authors: Huang Jingcheng , Chen Guoning , Hu Xiaoyan , Li Songhui , Wen Jingxiu , Liang Baowen , Jin Ying , Lu Yong , Lao Kete , Fu Changguan , Chen Runbo , Deng Honggui , Li Zhundong , Yang Zihang , Wu Yingzhu , Yang Min , Huang Hui , Tang Xiufeng , Luo Jianyi 
Published: November 2019

AbstractElectronic skins require to integrate multiple‐sensing functions to sense stimuli from the outside environment (such as pressure, temperature, and humidity), and to distinguish the signals from the different stimuli. Here, reported is the feasibility to use only one single carbon fiber beam (CFB) to simultaneously detect pressure and temperature changes from the outside environment, and the signals from pressure and temperature stimuli can be effectively distinguished through its transverse piezoresistance and longitudinal thermal resistance. This work also reveals that the transverse piezoresistance follows the electron quantum tunneling between the carbon fibers, while the longitudinal thermal resistance follows the impurity scattering mechanism. The flexible pressure sensors, temperature sensors, and their integrated sensors can be easily constructed like blocks to use the same material CFBs, and these sensors have demonstrated unique advantages in wearable healthcare monitoring devices and electronic skin products.
Impact of natural neuromedin‐B receptor variants on iron metabolism
Authors: Rametta Raffaela , Dongiovanni Paola , Baselli Guido A. , Pelusi Serena , Meroni Marica , Fracanzani Anna L. , Busti Fabiana , Castagna Annalisa , Scarlini Stefania , Corradini Elena , Pietrangelo Antonello , Girelli Domenico , Fargion Silvia , Valenti Luca 
Published: November 2019

AbstractIron overload heritability remains partly unexplained. By performing whole exome sequencing in three patients with a clinical phenotype of hemochromatosis not accounted by known genetic risk factors, we identified in all patients rare variants predicted to alter activity of Neuromedin‐B receptor (NMBR). Coding NMBR mutations were enriched in 129 patients with hereditary hemochromatosis or iron overload phenotype, as compared to ethnically matched controls, including 100 local healthy blood donors and 1000Genomes project participants (15.5% vs 5%, P = .0038 at burden test), and were associated with higher transferrin saturation in regular blood donors (P = .04). Consistently, in 191 patients with nonalcoholic fatty liver, the most common low‐frequency p.L390 M variant was independently associated with higher ferritin (P = .03). In 58 individuals, who underwent oral iron challenge, carriage of the p.L390 M variant was associated with higher transferrin saturation and lower hepcidin release. Furthermore, the circulating concentration of the natural NMBR ligand, Neuromedin‐B, was reduced in response to iron challenge. It was also decreased in individuals carrying the p.L390 M variant and with hemochromatosis in parallel with increased transferrin saturation. In mice, Nmbr was induced by chronic dietary iron overload in the liver, gut, pancreas, spleen, and skeletal muscle, while Nmb was downregulated in gut, pancreas and spleen. Finally, Nmb amplified holo‐transferrin dependent induction of hepcidin in primary mouse hepatocytes, which was associated with Jak2 induction and abolished by the NMBR antagonist PD168368. In conclusion, NMBR natural variants were enriched in patients with iron overload, and associated with facilitated iron absorption, possibly related to a defect of iron‐induced hepcidin release.
A Pd‐Catalyzed Three‐Component Reaction and Hydrogenation Strategy to Prepare 2‐Functionalized Cyclic Ethers Involving a Radical Initiating C(sp3)‐H Activation
Authors: Wan Zi‐juan , Yuan Xiao‐feng , Xiao Tian , Wang Xiao‐rong , Luo Jun 
Published: November 2019

AbstractA Pd‐catalyzed three‐component reaction and hydrogenation strategy to prepare a series of 2‐functionalized cyclic ethers with high to excellent yields was established. A plausible reaction mechanism for the three‐component coupling of N‐tosylhydrazones, aryl halides and cyclic ethers was proposed, which involves a radical initiating α‐C(sp3)‐H activation of the ethers and addition at β‐position of the palladium carbene‐derived α‐substituted styrenes. The resulting free radicals would undergo a disproportionation reaction to afford 2‐(2′,2′‐diarylvinyl)ethers and 2‐(2′,2′‐diarylethyl)ethers. The latter could also be formed from the oxidation of the free radicals via benzylic cations.
Tetrabenzononacene: “Butterfly Wings” Stabilize the Core
Authors: Müller Matthias , Maier Steffen , Tverskoy Olena , Rominger Frank , Freudenberg Jan , Bunz Uwe H. F. 
Published: November 2019

AbstractIn combination with bulky substituents at the core, fourfold benzannulation at the cata‐positions stabilizes a nonacene sufficiently to allow its isolation and characterization by 1H NMR and X‐ray analysis. The four benzo units blueshift the absorption spectrum in comparison to a solely linear nonacene, but significantly increase the stability in the solid state.
High‐Performance Quinoline‐Malononitrile Core as a Building Block for the Diversity‐Oriented Synthesis of AIEgens
Authors: Guo Zhiqian , Yan Chenxu , Zhu Wei‐Hong 
Published: November 2019

AbstractIn vivo fluorescent monitoring of physiological processes with high‐fidelity is essential in disease diagnosis and biological research, but faces extreme challenges due to aggregation‐caused quenching (ACQ) and short‐wavelength fluorescence. The development of high‐performance and long‐wavelength aggregation‐induced emission (AIE) fluorophores is in high demand for precise optical bioimaging. The chromophore quinoline‐malononitrile (QM) has recently emerged as a new class of AIE building block that possesses several notable features, such as red to near‐infrared (NIR) emission, high brightness, marked photostability, and good biocompatibility. In this minireview, we summarize some recent advances of our established AIE building block of QM, focusing on the AIE mechanism, regulation of emission wavelength and morphology, the facile scale‐up and fast preparation for AIE nanoparticles, as well as potential biomedical imaging applications.
Retracted: The Manganese(I)‐Catalyzed Asymmetric Transfer Hydrogenation of Ketones: Disclosing the Macrocylic Privilege
Authors: Passera Alessandro , Mezzetti Antonio 
Published: November 2019

AbstractThe bis(carbonyl) manganese(I) complex [Mn(CO)2(1)]Br (2) with a chiral (NH)2P2 macrocyclic ligand (1) catalyzes the asymmetric transfer hydrogenation of polar double bonds with 2‐propanol as the hydrogen source. Ketones (43 substrates) are reduced to alcohols in high yields (up to >99 %) and with excellent enantioselectivities (90–99 % ee). A stereochemical model based on attractive CH–π interactions is proposed.
Preparation and Characterization of Oil Rich in Odd Chain Fatty Acids fromRhodococcus opacusPD630
Authors: Chu Mei‐Yun , Zhang Lin‐Shang , Lou Wen‐Yong , Zong Min‐Hua , Tang Yu‐Qian , Yang Ji‐Guo 
Published: November 2019

AbstractOdd‐chain fatty acids (OCFA) are widely used in pharmaceutical and food industries, as well as the chemical industry. In this study, oil rich in OCFA was produced byRhodococcus opacusPD630, and 1‐propanol in combination with glucose was used as the carbon and energy source. Oil was extracted by the subcritical extraction system. Gas chromatography (GC), ultra‐performance liquid chromatography‐mass spectrometry (UPLC‐MS), and differential scanning calorimetry (DSC) were used to evaluate the fatty acids composition, triacylglycerol (TAG) molecular species, and thermal properties, respectively. It was found that the content of OCFA reached 68.15%, where pentadecanoic acid (7.74%), heptadecanoic acid (18.20%), and heptadecenoic acid (42.71%) were the dominant OCFA. The most abundant TAG were HePaHa (15.82%), HePHa (11.42%), HePaHe (9.68%), HeHaHa (9.62%), HePaO (8.26%), and HePPa (8.00%). In terms of thermal profiles, recrystallization was detected in the oil rich in OCFA. Above all, these findings greatly extend the utilization of the microbial oil rich in OCFA, and they may have a significant impact on the future development of the microbial oil industry.
Hunters'structured questionnaires enhance ecological knowledge and provide circumstantial survival evidence for the world's rarest turtle
Authors: Pham Van Thong , Le Duc Olivier , Leprince Benjamin , Bordes Cedric , Luu Vinh Quang , Luiselli Luca 
Published: November 2019

Abstract

Rafetus swinhoei, a giant softshell turtle from southern China and northern Vietnam, is the rarest turtle species on Earth, and only two individuals are known worldwide (one in the wild). There is very little ecological information for this species, and the remnant distribution remains completely unknown.
This article reports for the first time in the international literature the results of a structured questionnaire survey concerning the ecology and distribution of R. swinhoei in Vietnam. The standardized questionnaire consisted of 15 questions and was administered to 10 former Rafetus hunters with several years of experience in catching this species.
There was considerable consensus among the hunters regarding several aspects of the biology of R. swinhoei, with special reference to its reproductive and feeding habits. The mean weight of 58 captured individuals was 53 kg, with the majority of individuals weighing 15–40 kg (median = 40 kg) and only 17.2% of individuals weighing more than 100 kg.
Based on the hunters'answers, it was established that the population collapse of R. swinhoei in Vietnam occurred in two phases: first during the 1980s, when the population size dramatically decreased, and second, at the beginning of the 1990s, when many hunters simultaneously collected multiple individuals from populations already experiencing severe impacts. There was also a general agreement that these turtles became very rare after 1991–92.
In total, 90% of the hunters agreed that there should still be wild R. swinhoei individuals at their respective sites, and some of the hunters also reported a few recent sightings that they attributed to the target species.
As several sites of likely presence were identified, we suggest that extensive trapping should be planned at these sites in future.
Highly Nitrogen‐Doped Porous Carbon Nanosheets as High‐Performance Anode for Potassium‐Ion Batteries
Authors: Zhang Dong Mei , Chen Zhi Wen , Bai Jie , Yang Chun Cheng , Jiang Qing 
Published: November 2019

AbstractPotassium‐ion batteries (PIBs) represent a promising alternative to lithium‐ion batteries owing to the high abundance and low cost of potassium resources. However, it is still a challenge to design anode materials with superior electrochemical performance because of the intrinsic large ionic radius and heavy molar mass of K+. Here, highly nitrogen‐doped porous carbon nanosheets (N‐PCNs) were fabricated through a template‐removal method. As an anode in PIBs, N‐PCNs exhibits superior rate property of 127.5 mAh g−1 at a current density of 5 A g−1 and cycling stability of 151.2 mAh g−1 after 10000 cycles at 1 A g−1. These remarkable performances originate from ultra‐high N doping (12 at%) as well as high specific surface area (848.3 m2 g−1) with abundant hierarchical pores, which supply numerous active sites for K+ insertion/extraction, enhance the electronic conductivity, and also offer efficient electrolyte diffusion paths. Density functional theory calculations demonstrate that pyrrolic‐ and pyridinic‐N doping can efficiently promote the adsorption of K+ and reduce volume expansion after the insertion of K+, leading to high specific capacity and superior cycling stability of N‐PCNs.
Reactive oxygen species responsive nanoplatforms as smart drug delivery systems for gastrointestinal tract targeting
Authors: Bertoni Serena , Machness Ariella , Tiboni Mattia , Bártolo Raquel , Santos Hélder A. 
Published: November 2019

AbstractThe pharmacological therapy for gastrointestinal (GI) diseases, such as inflammatory bowel diseases, continues to present challenges in targeting efficacy. The need for maximal local drug exposure at the inflamed regions of the GI tract has led research to focus on a disease‐targeted drug delivery approach. Smart nanomaterials responsive to the reactive oxygen species (ROS) concentrated in the inflamed areas, can be formulated into nanoplatforms to selectively release the active compounds, avoiding unspecific drug delivery to healthy tissues and limiting systemic absorption. Recent developments of ROS‐responsive nanoplatforms include combination with other materials to obtain multi‐responsive systems and modifications/derivatization to increase the interactions with biological tissues, cell uptake and targeting. This review describes the applications of ROS‐responsive nanosystems for on‐demand drug delivery to the GI tract.
Pityriasis rubra pilaris‐like graft‐vs‐host disease following allogeneic stem cell transplant in two patients
Authors: Wang Jennifer Y. , Tabata Mika M. , Pugliese Silvina , Phillips Darci , Kim Jinah , Weng Wen‐Kai , Kwong Bernice Y. 
Published: November 2019

AbstractChronic cutaneous graft‐vs‐host disease (GVHD) has several atypical variants. We describe two cases of GVHD with clinical and histopathologic features of pityriasis rubra pilaris (PRP), which responded to additional immunosuppression. Recognition of this newly described PRP‐like clinical presentation of GVHD may prompt early consideration of additional steroid‐sparing therapies.
Pelvic Schwannoma: In light of a case report
Authors: Pak Haleh , Haji Maghsoudi Leila 
Published: November 2019

AbstractA 41‐year‐old man with abdominal enlargement, as a result of large retroperitoneal Schwannoma, was reported to our center. It was seen to have caused the displacement of the colon and was attached to the presacral region of the spine. Following the diagnosis, successful resection of Schwannoma was achieved.