bluuebunny/crossref_metadata_2025_split huggingface dataset:
Construccionismo Social: Aplicación del Grupo de Discusión en Praxis de Equipo Reflexivo en la Investigación Científica
Authors: Donoso Niemeyer Tania 
Published: October 2020

Este trabajo ilustra cómo la contribución de la técnica de Grupo de Discusión en el marco de una praxis del Equipo Reflexivo de Andersen puede ser utilizada como un método útil en la investigación científica cualitativa. Los postulados teóricos provienen del Construccionismo Social, desde el cual es posible conocer los significados y atribuciones que las propias personas señalan en relación a un determinado tema o problema.El Construccionismo Social estudia los fenómenos psicológicos a partir del modo que las personas aplican en su experiencia presente, las vivencias personales de su historia de vida, las influencias del contexto social y el conocimiento teórico en un determinado tema.Se ilustra el uso de este método de investigación en una experiencia de Grupo de Discusión con formato de Equipo Reflexivo, realizada con un grupo de 6 madres con niños entre 5 y 8 años. El propósito fue explorar los relatos de experiencia y las atribuciones de significado que ellas señalaban como sus principales recursos y debilidades en la crianza de sus hijos.
La Experiencia del Campus Virtual y la Evaluación del Uso que realizan los Estudiantes de Psicologia de la Red de Internet.
Authors: Ahumada Figueroa Luis 
Published: October 2020

En este trabajo, se presenta la incorporación de un recurso de aprendizaje virtual a la asignatura de Psicología Social disponible en la página del Campus Virtual de la Universidad Católica de Valparaíso- Chile.El sitio, dirigido a estudiantes de segundo año que cursan la asignatura mencionada, incorporó áreas temáticas no abordadas en los cursos regulares y una metodología de trabajo que favoreciera el desarrollo de una Comunidad de Aprendizaje integrando distintas perspectivas disciplinarias en el abordaje de problemas psicosociales.Específicamente, en el marco de un Aula Virtual complementaria a las clasespresenciales, se desarrollaron una serie de recursos de comunicación tales como foros de discusión, votaciones respecto a problemáticas vistas en clase y chat de conversación.Se incorporaron también una serie de recursos propios de la asignaturatales como textos de apoyo y material electrónico propios del curso así como recursos de información útiles para la búsqueda de información en Psicología (PsycFirst, PsycINFO_1887, Econlit, entre otros).Al comienzo y al final del curso (anual), se evaluó el impacto de esta metodología en el uso que realizan los estudiantes de la red de Internet, aplicando una encuesta elaborada específicamente para evaluar la utilización de la red de Internet por parte e los estudiantes.Mediante esta metodología de enseñanza aprendizaje se logró que el estudiante incorporara información adicional a los temas vistos en Cátedra, además se observó un cambio significativo en la evaluación respecto a la finalidad del uso de la Red y su utilización como recurso de aprendizaje.La utilización de esta herramienta virtual y esta nueva metodología de enseñanzaaprendizaje, mejora la calidad de la docencia, no tanto en el aprendizaje de los contenidos propios de la Psicología Social, como en el desarrollo de competencias transversales relacionadas principalmente con la gestión de la información y el conocimiento en el contexto de las Tecnologías de la Información y la Comunicación (TICs).
Algunos Factores de Riesgo en la Salud Mental de la Mujer Rural Temporera y su abordaje desde la Terapia Grupal
Authors: Garay Moffat Verónica 
Published: October 2020

En este artículo se abordan factores de riesgo psicosocial dentro del contexto de laterapia grupal dirigida mujeres rurales temporeras, que por la especificidad de sutarea, y por las características del tipo de familias al que pertenecen, requieren unanálisis de la situación problema desde un enfoque cada vez más participativo, conun énfasis en la autogestión, género y desarrollo humano. Se revisan diversos autores, encontrándose en éstos un referente de terapia que pone el acento en la reconstrucción de la identidad perdida, en lo que habitualmente rotulamos como una entidad estática y abordamos desde un esquema tradicional. Se comparan dos formas de abordaje terapéutico, transfiriendo un modelo terapéutico al tratamiento de la depresión.
A sequential explanatory mixed methods study design: An example of how to integrate data in a midwifery research project
Authors: Othman Shwikar , Steen Mary , Fleet Julie-Anne 
Published: October 2020

Integration of mixed methods involves bringing together quantitative and qualitative approaches and methodologies. Limited application in midwifery research has identified a need for practical examples. How to integrate two research approaches and methodologies in a sequential explanatory mixed methods study, at the design, methods, interpretation and reporting levels will be explained. This paper describes and discusses an example of how integration was used to develop a better understanding of midwives’ knowledge and confidence after attending a healthy eating education workshop/webinar. This example illustrates how integration can be achieved and emphasises how a weaving technique can be used, and findings are presented in a joint display and extreme case analysis. The sequential explanatory design was adopted to merge and mix different datasets to be collected and analysed. Then, using meta-analysis to identify areas of convergence or discordance, which provided a more comprehensive overview and understanding of the key themes that linked midwives' knowledge and confidence. The application of this mixed methods design assisted in investigating and exploring midwives' knowledge and confidence levels and provided clear insights for midwives needs and the effectiveness of healthy eating education on practice.
Optimiser les soins palliatifs précoces aux patients atteints d’un cancer hématologique recevant une greffe de cellules souches : rôle de l’infirmière praticienne
Authors: Lewis Ellen 
Published: October 2020

La greffe de cellules souches hématopoïétiques (GCSH) est une approche thérapeutique intensive des hémopathies malignes qui s’accompagne d’un important risque de morbidité et de mortalité. Malgré la lueur d’espoir qu’elle apporte aux patients, les traitements sont longs et pénibles, tant du point de vue physique que psychosocial. Habituellement, la greffe de cellules souches hématopoïétiques a une visée curative qui laisse peu de place à un partenariat éventuel avec les services de soins palliatifs et, lorsque ces derniers sont proposés, il est souvent trop tard pour en tirer un bénéfice notable. Il faudrait donc systématiser le recours aux soins palliatifs pour les patients atteints d’hémopathie maligne qui reçoivent une GCSH; cependant, il demeure difficile de trouver la meilleure voie pour établir un tel partenariat. Les infirmières praticiennes, qui sont de plus en plus nombreuses, possèdent les connaissances et les compétences requises pour combler les lacunes dans la prestation de soins palliatifs aux patients recevant une GCSH. L’algorithme de soins palliatifs précoces dispensés par les infirmières praticiennes aux patients recevant une GCSH (dit NEST en anglais, pour « Nurse-Practitioner-Delivered Early Palliative Care for Stem Cell Transplant ») vise justement à faire le pont entre les services palliatifs et les équipes d’hématologie et de greffe afin d’offrir aux patients les meilleurs soins possible.
Pan-TRK Immunohistochemistry: An Example-Based Practical Approach to Efficiently Identify Patients With NTRK Fusion Cancer
Authors: Conde Esther , Hernandez Susana , Sanchez Elena , Regojo Rita Maria , Camacho Carmen , Alonso Marta , Martinez Rebeca , Lopez-Rios Fernando 
Published: October 2020

Context.—
Food and Drug Administration–approved TRK inhibitors with impressive overall response rates are now available for patients with multiple cancer types that harbor NTRK rearrangements, yet the identification of NTRK fusions remains a difficult challenge. These alterations are highly recurrent in extremely rare malignancies or can be detected in exceedingly small subsets of common tumor types. A 2-step approach has been proposed, involving a screening by immunohistochemistry (IHC) followed by a confirmatory method (fluorescence in situ hybridization, reverse transcriptase–polymerase chain reaction, or next-generation sequencing) in cases expressing the protein. However, there is no interpretation guide for any of the available IHC clones.

Objective.—
To provide a pragmatic update on the use of pan-TRK IHC. Selected examples of the different IHC staining patterns across multiple histologies are shown.

Data Sources.—
Primary literature review with PubMed, combined with personal diagnostic and research experience.

Conclusions.—
In-depth knowledge of pan-TRK IHC will help pathologists implement a rational approach to the detection of NTRK fusions in human malignancies.
Effect of Immobilization Methods on the Production of Polyethylene-cellulose Biocomposites via Ethylene Polymerization with Metallocene/MAO Catalyst
Authors: Tumawong Praonapa , Chaichana Ekrachan , Jongsomjit Bunjerd 
Published: October 2020

Polyethylene-cellulose biocomposites were synthesized here via the ethylene polymerization with metallocene as a catalyst along with methylaluminoxane (MAO) as a cocatalyst. The immobilization method in which the catalyst or cocatalyst is fixed onto the catalytic filler (cellulose) can be classified into 3 methods according to the active components fixed onto the filler surface: 1) only metallocene catalyst (Cellulose/Zr), 2) only MAO cocatalyst (Cellulose/MAO) and 3) mixture of metallocene and MAO (Cellulose/(Zr+MAO)). It was found that the different immobilization methods or different fillers altered the properties of the obtained composites and also the catalytic activity of the polymerization systems. It was found that Cellulose/MAO provided the highest catalytic activity among all fillers due to a crown-alumoxane complex, which caused the heterogeneous system with this filler behaved similarly to the homogeneous system. The different fillers also produced the biocomposites with some different properties such as crystallinity which Cellulose/Zr provided the highest crystallinity compared with other fillers as observed by a thermal gravimetric analysis-differential scanning calorimetry (TGA-DSC). Nevertheless, the main crystal structure indicated to the typical polyethylene was still observed for all obtained biocomposites with different fillers as observed by an X-ray diffractometer (XRD). Copyright © 2021 by Authors, Published by BCREC Group. This is an open access article under the CC BY-SA License (https://creativecommons.org/licenses/by-sa/4.0).
The Saccharomyces cerevisiae ABC subfamily D transporter Pxa1/Pxa2p co‐imports CoASH into the peroxisome
Authors: van Roermund Carlo W. T. , IJlst Lodewijk , Baker Alison , Wanders Ronald J. A. , Theodoulou Freddie L. , Waterham Hans R. 
Published: October 2020

ATP‐binding cassette (ABC) subfamily D transporters are important for the uptake of fatty acids and other beta‐oxidation substrates into peroxisomes. Genetic and biochemical evidence indicates that the transporters accept fatty acyl‐coenzyme A that is cleaved during the transport cycle and then re‐esterified in the peroxisomal lumen. However, it is not known whether free coenzyme A (CoA) is released inside or outside the peroxisome. Here we have used Saccharomyces cerevisiae and isolated peroxisomes to demonstrate that free CoA is released in the peroxisomal lumen. Thus, ABC subfamily D transporter provide an import pathway for free CoA that controls peroxisomal CoA homeostasis and tunes metabolism according to the cell’s demands.
Occludin‐binding single‐chain variable fragment and antigen‐binding fragment antibodies prevent hepatitis C virus infection
Authors: Shimizu Yoshimi , Shinoda Takehiro , Shirasago Yoshitaka , Kondoh Masuo , Shinya Naoko , Hanada Kentaro , Yagi Kiyohito , Suzuki Tetsuro , Wakita Takaji , Kimura‐Someya Tomomi , Shirouzu Mikako , Fukasawa Masayoshi 
Published: October 2020

Occludin (OCLN) is a tetraspan membrane component of epithelial tight junctions and a known receptor for hepatitis C virus (HCV). Previously, we established functional monoclonal antibodies (mAbs) that bind to each extracellular loop of OCLN and showed their ability to prevent in vitro and in vivo HCV infection. In this study, we converted these mAbs to corresponding monovalent antigen‐binding fragments (Fabs) and single‐chain variable fragment (scFv) antibodies. These Fab fragments and scFv antibodies demonstrate similar binding specificity and affinity to parental anti‐OCLN mAbs. Moreover, Fab fragments and scFv antibodies inhibit in vitro HCV infection. The small functional monovalent OCLN‐binding probes reported in our study have high potential as drug candidates and tools for biological and pharmaceutical studies of OCLN.
The role of the cytoskeletal proteins MreB and FtsZ in multicellular cyanobacteria
Authors: Springstein Benjamin L. , Weissenbach Julia , Koch Robin , Stücker Fenna , Stucken Karina 
Published: October 2020

Multiseriate and true‐branching cyanobacteria are at the peak of prokaryotic morphological complexity. However, little is known about the mechanisms governing multiplanar cell division and morphogenesis. Here, we study the function of the prokaryotic cytoskeletal proteins, MreB and FtsZ in Fischerella muscicola PCC 7414 and Chlorogloeopsis fritschii PCC 6912. Vancomycin and HADA labeling revealed a mixed apical, septal, and lateral trichome growth mode in F. muscicola, whereas C. fritschii exhibits septal growth. In all morphotypes from both species, MreB forms either linear filaments or filamentous strings and can interact with FtsZ. Furthermore, multiplanar cell division in F. muscicola likely depends on FtsZ dosage. Our results lay the groundwork for future studies on cytoskeletal proteins in morphologically complex cyanobacteria.
On the optimization of bone SPECT/CT in terms of image quality and radiation dose
Authors: Tulik Monika , Tulik Piotr , Kowalska Teresa 
Published: October 2020

AbstractIntroductionThe purpose of this study was to present the optimization process of CT parameters to reduce patient exposure during bone SPECT/CT without affecting the quality of SPECT images with attenuation correction (AC).Material and methodsA fillable phantom reflecting realistic bone scintigraphy conditions was developed and acquired on an AnyScan SC. SPECT/CT scans were carried out with different x‐ray tube current values (10, 20, 30, 40, 50, 60, 70, 90, 110, 130, 150, and 200 mA) at three different high‐voltage values (80, 100, and 120 kV). The contrast (C) and coefficients of variation (CV) in the SPECT images as well as the signal‐to‐noise ratio (SNR) and noise (SDCT) in the CT images with CTDIvol were measured. An optimal acquisition protocol that obtained SPECT/CT images with no artifacts on both CT and SPECT images, acceptable C, SNR, CV, and SDCT values, and the largest reduction in patient exposure compared to the reference acquisition procedure was sought.ResultsThe optimal set of parameters for bone SPECT/CT was determined based on a phantom study. It has been implemented in clinical practice. Two groups of patients were examined according to the baseline and optimized protocols, respectively. The new SPECT/CT protocol substantially reduced patients’ radiation exposure compared to the old protocol while maintaining the required diagnostic quality of SPECT and CT images.ConclusionsIn the study, we present a methodology that finds a compromise between diagnostic information and patient exposure during bone SPECT/CT procedures.
Development of a New Bone‐Mimetic Surface Treatment Platform: Nanoneedle Hydroxyapatite (nnHA) Coating
Authors: Eichholz Kian F. , Von Euw Stanislas , Burdis Ross , Kelly Daniel J. , Hoey David A. 
Published: October 2020

AbstractThe hierarchical structure of bone plays pivotal roles in driving cell behavior and tissue regeneration and must be considered when designing materials for orthopedic applications. Herein, it is aimed to recapitulate the native bone environment by using melt electrowriting to fabricate fibrous microarchitectures which are modified with plate‐shaped (pHA) or novel nanoneedle‐shaped (nnHA) crystals. Nuclear magnetic resonance spectroscopy, scanning electron microscopy, transmission electron microscopy, and X‐ray diffraction demonstrate that these coatings replicate the nanostructure and composition of native bone. Human mesenchymal stem/stromal cell (MSC) mineralization is significantly increased fivefold with pHA scaffolds and 14‐fold with nnHA scaffolds. Given the protein stabilizing properties of mineral, these materials are further functionalized with bone morphogenetic protein 2 (BMP2). nnHA treatment facilitates controlled release of BMP2 which further enhance MSC mineral deposition. Finally, the versatility of this nnHA treatment method, which may be used to coat different architectures/materials including fused deposition modeling (FDM) scaffolds and Ti6Al4V titanium, is demonstrated. This study thus outlines a method for fabricating scaffolds with precise fibrous microarchitectures and bone‐mimetic nnHA extrafibrillar coatings which significantly enhance MSC osteogenesis and therapeutic protein delivery, and leverages these results to show how this surface treatment method may be applied to a wider field for multiple orthopedic applications.
Origin of High Nonradiative Recombination and Relevant Optoelectronic Properties of Ba2Bi1+xNb1−xO6: Candidate for Photo(electro)catalysis and Photovoltaic Applications?
Authors: Kangsabanik Jiban , Borkar Hitesh , Bhawna  , Siddiqui Mohd Salman , Aslam M. , Alam Aftab 
Published: October 2020

AbstractRecently, perovskite oxides have shown a lot of promise as active materials for photovoltaic (PV) and photoelectrochemical (PEC) devices. In this article, a series of oxides Ba2Bi1+xNb1−xO6 (0 ≤ x ≤ 0.8) is evaluated for PV/PEC applications via a combined theoretical and experimental study. Ab‐initio calculation reveals that the stoichiometric compound (Ba2BiNbO6) spontaneously induces the formation of BiNb antisites, leading to off‐stoichiometric structure. The electronic structure of off‐stoichiometric compounds confirms mixed valence character of Bi, introducing intermediate bands in the band structure. UV–vis diffuse reflectance spectroscopy (DRS) predicts the bandgap in the visible range (1.5–1.9 eV). Simulation reveals excellent absorption properties, indicating the possibility of high photoconversion efficiency. Lack of sharp peaks in the DRS, however, raises concern about the level of nonradiative recombination. Point defect simulation suggests the possibility of few deep level donors BiBa, NbBi, VO, in high concentrations (≈1018–1019 cm−3), which can act as nonradiative recombination centers. Ultraviolet photoelectron spectroscopy confirms majority carriers to be n‐type. Self‐consistent simulation of the Fermi level (EF) suggests an overall cancellation of donors and acceptors, leading to EF pinning at the mid‐gap region explaining the observed high bulk resistivity. This indicates the requirement of extrinsic doping to achieve desired properties for applications.
Transcranial Ultrasound Innovations Ready for Broad Clinical Application
Authors: Beisteiner Roland , Lozano Andres M. 
Published: October 2020

AbstractBrain diseases are one of the most important problems in our rapidly ageing society. Currently, there are not many effective medications and surgical options are limited due to invasiveness and non‐invasive brain stimulation techniques cannot be well targeted and cannot access deep brain areas. A novel therapy is transcranial ultrasound which allows a variety of treatments without opening of the skull. Recent technological developments generated three revolutionary options including 1) targeted non‐invasive surgery, 2) highly targeted drug, antibody, or gene therapy via local opening of the blood–brain barrier, and 3) highly targeted brain stimulation to improve pathological brain functions. This progress report summarizes the current state of the art for clinical application and the results of recent patient investigations.
Comparison of the continuation and discontinuation of perioperative antiplatelet therapy in laparoscopic surgery for colorectal cancer: A retrospective, multicenter, observational study (YCOG 1603)
Authors: Ohya Hiroki , Watanabe Jun , Suwa Yusuke , Nakagawa Kazuya , Suwa Hirokazu , Ozawa Mayumi , Ishibe Atsushi , Kunisaki Chikara , Endo Itaru 
Published: October 2020

AbstractAimThe present study aimed to examine the effect of continuing antiplatelet therapy in the perioperative period for patients undergoing laparoscopic resection for colorectal cancer who had received preoperative antiplatelet therapy.MethodsThis retrospective, multicenter, observational study included patients who underwent laparoscopic surgery for colorectal cancer between January 2011 and May 2020. The study population was limited to patients who used antiplatelet therapy preoperatively.ResultsA total of 214 colorectal cancer patients who received antiplatelet therapy preoperatively were included in the present study. Eighty‐nine patients underwent surgery under the continuation of antiplatelet therapy, and 125 patients underwent surgery under the discontinuation of antiplatelet therapy before surgery. There were no significant differences between the two groups with regard to intraoperative blood loss (P = .889), intraoperative blood transfusion (P = 1.000), and conversion to laparotomy (P = 1.000). There were no significant differences between the two groups in the incidence of postoperative hemorrhagic complications (Clavien‐Dindo Grade ≥II, P = .453; Grade ≥III, P = .572) or three‐point major adverse cardiovascular events (P = .268). However, there were two cases of postoperative non‐fatal stroke in the discontinued antiplatelet therapy group.ConclusionsThe present study revealed that there were no significant differences in the surgical outcomes and postoperative complications between colorectal cancer patients who underwent laparoscopic resection with the continuation of antiplatelet therapy in the perioperative period and those in whom antiplatelet therapy was discontinued during the perioperative period. From the viewpoint of cardiovascular and cerebrovascular risk, it may be better for patients undergoing laparoscopic surgery for colorectal cancer to continue antiplatelet therapy. This study was registered with the Japanese Clinical Trials Registry as UMIN000038707 (http://www.umin.ac.jp/ctr/index.htm).