bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Review on Investigating the experimental process for partial replacement of cement with sugarcane bagasse in the construction industry
Authors: Dawoud Nadin , Micheal Amany , Moussa Rania Rushdy 
Published: December 2020

Abstract
In the last few decades there has been speedily increasing in the agriculture and industrial wastes. This causes many environmental issues and raises the potential to contaminate the natural resources of living such as water, air and soil. Recently, the amount of organic waste produced daily has been rising, while it is poorly managed. It is either burned or disposed improperly, which effect negatively the environment and public health. On the other hand, during the cement production process many wastes, and pollutants are generated which have major negative impacts on the environment. Cement is considered as a substantial constituent of building materials in the construction industry. Many research’s intended to explore the potential of utilizing organic waste products in the construction industry by partially replacing cement with organic wastes such as sugarcane bagasse to create an eco-friendly brick with appropriate structure properties. Previous research’s used different treatment techniques to treat the organic waste and prepare it for construction industry. It was noticed that the treatment method used in previous research’s affected the structural properties of the new bricks with organic waste partially replacing. This research intends to study and analyse the process and techniques used by other researcher’s experimental work to treat and replace cement with sugarcane bagasse. This research will present the best procedures for partially replacing cement with sugarcane bagasse in cement bricks without compensating the structural properties of regular cement bricks. This study will analyse and compare previous researcher’s experimental work to obtain the best experimental program and to utilize sugarcane bagasse in brick industry.
IV—Moral Knowledge and Empirical Investigation in Late Ming China
Authors: Jenco Leigh K 
Published: December 2020

AbstractThis essay begins to explore the philosophical grounds on which Chinese literati thinkers came to legitimate, and in some cases value, alternative ways of life in the early modern era (sixteenth and seventeenth centuries). In this essay I examine arguments from two such scholars, the flamboyant iconoclast Li Zhi 李贄 (1527–1602) and his lifelong friend, the historian and classicist Jiao Hong 焦竑 (1540–1620), to show how this interest in the empirical world led them away from their commitments to moral universalism and toward an appreciation of the diversity and plurality of human moral values.
ExonSkipAD provides the functional genomic landscape of exon skipping events in Alzheimer’s disease
Authors: Yang Mengyuan , Ke Yiya , Kim Pora , Zhou Xiaobo 
Published: December 2020

Abstract
Exon skipping (ES), the most common alternative splicing event, has been reported to contribute to diverse human diseases due to the loss of functional domains/sites or frameshifting of the open reading frame (ORF) and noticed as therapeutic targets. Accumulating transcriptomic studies of aging brains show the splicing disruption is a widespread hallmark of neurodegenerative diseases such as Alzheimer’s disease (AD). Here, we built ExonSkipAD, the ES annotation database aiming to provide a resource/reference for functional annotation of ES events in AD and identify therapeutic targets in exon units. We identified 16 414 genes that have ~156 K, ~ 69 K, ~ 231 K ES events from the three representative AD cohorts of ROSMAP, MSBB and Mayo, respectively. For these ES events, we performed multiple functional annotations relating to ES mechanisms or downstream. Specifically, through the functional feature retention studies followed by the open reading frames (ORFs), we identified 275 important cellular regulators that might lose their cellular regulator roles due to exon skipping in AD. ExonSkipAD provides twelve categories of annotations: gene summary, gene structures and expression levels, exon skipping events with PSIs, ORF annotation, exon skipping events in the canonical protein sequence, 3′-UTR located exon skipping events lost miRNA-binding sites, SNversus in the skipped exons with a depth of coverage, AD stage-associated exon skipping events, splicing quantitative trait loci (sQTLs) in the skipped exons, correlation with RNA-binding proteins, and related drugs & diseases. ExonSkipAD will be a unique resource of transcriptomic diversity research for understanding the mechanisms of neurodegenerative disease development and identifying potential therapeutic targets in AD. Significance AS the first comprehensive resource of the functional genomics of the alternative splicing events in AD, ExonSkipAD will be useful for many researchers in the fields of pathology, AD genomics and precision medicine, and pharmaceutical and therapeutic researches.
Decoding the role of long noncoding RNAs in the healthy aging of centenarians
Authors: Jiang Jianjun , Cheng Lehua , Yan Liang , Ge Mingxia , Yang Liqin , Ying Haoqiang , Kong Qingpeng 
Published: December 2020

Abstract
Aging is the largest risk factor of major human diseases. Long noncoding RNAs (lncRNAs) as the key regulatory elements have shown a strong impact on multiple biological processes as well as human disease mechanisms. However, the roles of lncRNAs in aging/healthy aging processes remain largely unknown. Centenarians are good models for healthy aging studies due to avoiding major chronic diseases and disabilities. To illustrate their ubiquitous nature in the genome and the ‘secrets’ of healthy aging regulation from the perspective of lncRNAs, peripheral blood samples from two regions consisting 76 centenarians (CENs), 54 centenarian-children (F1) and 41 spouses of centenarian-children (F1SP) were collected for deep RNA-seq. We identified 11 CEN-specific lncRNAs that is particularly expressed in longevous individuals. By kmers clustering, hundreds of human lncRNAs show similarities with CEN-specific lncRNAs, especially with ENST00000521663 and ENST00000444998. Using F1SP as normal elder controls (age: 59.9 ± 6.6 years), eight lncRNAs that are differentially expressed in longevous elders (CEN group, age: 102.2 ± 2.4 years) were identified as candidate aging/health aging–related lncRNAs (car-lncs). We found that the expression of eight car-lncs in human diploid fibroblasts displayed dynamic changes during cell passage and/or H2O2/rapamycin treatment; of which, overexpression either of THBS1-IT1 and THBS1-AS1, two lncRNAs that highly expressed in CENs, can remarkably decrease p16, p21 and the activity of senescent related β-galactosidase, suggesting that THBS1-IT1 and THBS1-AS1 can inhibit cellular senescence. We provided the first comprehensive analysis of lncRNA expression in longevous populations, and our results hinted that dysregulated lncRNAs in CENs are potential protective factors in healthy aging process.
Penalized model-based clustering of fMRI data
Authors: Dilernia Andrew , Quevedo Karina , Camchong Jazmin , Lim Kelvin , Pan Wei , Zhang Lin 
Published: December 2020

AbstractFunctional magnetic resonance imaging (fMRI) data have become increasingly available and are useful for describing functional connectivity (FC), the relatedness of neuronal activity in regions of the brain. This FC of the brain provides insight into certain neurodegenerative diseases and psychiatric disorders, and thus is of clinical importance. To help inform physicians regarding patient diagnoses, unsupervised clustering of subjects based on FC is desired, allowing the data to inform us of groupings of patients based on shared features of connectivity. Since heterogeneity in FC is present even between patients within the same group, it is important to allow subject-level differences in connectivity, while still pooling information across patients within each group to describe group-level FC. To this end, we propose a random covariance clustering model (RCCM) to concurrently cluster subjects based on their FC networks, estimate the unique FC networks of each subject, and to infer shared network features. Although current methods exist for estimating FC or clustering subjects using fMRI data, our novel contribution is to cluster or group subjects based on similar FC of the brain while simultaneously providing group- and subject-level FC network estimates. The competitive performance of RCCM relative to other methods is demonstrated through simulations in various settings, achieving both improved clustering of subjects and estimation of FC networks. Utility of the proposed method is demonstrated with application to a resting-state fMRI data set collected on 43 healthy controls and 61 participants diagnosed with schizophrenia.
Risk of Acute Kidney Injury andClostridioides difficileInfection With Piperacillin/Tazobactam, Cefepime, and Meropenem With or Without Vancomycin
Authors: Lee Jazmin D , Heintz Brett H , Mosher Hilary J , Livorsi Daniel J , Egge Jason A , Lund Brian C 
Published: December 2020

AbstractBackgroundEmpiric antimicrobial therapy for healthcare-acquired infections often includes vancomycin plus an anti-pseudomonal beta-lactam (AP-BL). These agents vary in risk for adverse events, including acute kidney injury (AKI) and Clostrioides difficile infection (CDI). Studies have only examined these risks separately; thus, our objective was to evaluate AKI and CDI risks simultaneously with AP-BL in the same patient cohort.MethodsThis retrospective cohort study included 789 200 Veterans Health Administration medical admissions from 1 July 2010 through 30 June 2016. The antimicrobials examined were vancomycin, cefepime, piperacillin/tazobactam, and meropenem. Cox proportional hazards regression was used to contrast risks for AKI and CDI across individual target antimicrobials and vancomycin combination therapies, including adjustment for known confounders.ResultsWith respect to the base rate of AKI among patients who did not receive a target antibiotic (4.6%), the adjusted hazards ratios for piperacillin/tazobactam, cefepime, and meropenem were 1.50 (95% CI: 1.43–1.54), 1.00 (.95–1.05), 0.92 (.83–1.01), respectively. Co-administration of vancomycin increased AKI rates (data not shown). Similarly, against the base rate of CDI (0.7%), these ratios were 1.21 (1.07–1.36), 1.89 (1.62–2.20), and 1.99 (1.55–2.56), respectively. Addition of vancomycin had minimal impact on CDI rates (data not shown).ConclusionsPiperacillin/tazobactam increased AKI risk, which was exacerbated by concurrent vancomycin. Cefepime and meropenem increased CDI risk relative to piperacillin/tazobactam. Clinicians should consider the risks and benefits of AP-BL when selecting empiric regimens. Further well-designed studies evaluating the global risks of AP-BL and patient specific characteristics that can guide empiric selection are needed.
Dietary Pattern Trajectories in Middle Age and Physical Function in Older Age
Authors: Talegawkar Sameera A , Jin Yichen , Xue Qian-Li , Tanaka Toshiko , Simonsick Eleanor M , Tucker Katherine L , Ferrucci Luigi 
Published: December 2020

Abstract

Background
Increasingly, lifestyle factors in midlife are reported to impact health and functional status in old age. This work examines associations between dietary trajectories in middle age and subsequent impairments in physical function.

Method
Data are from 851 participants (61% men, mean age at first dietary assessment = 47 years, range 30–59 years) from the Baltimore Longitudinal Study of Aging. We used latent class analysis to derive dietary trajectories based on adherence to the Alternative Healthy Eating Index-2010 (AHEI), and further classified them based on tertiles, as poor (score &lt;39.3), intermediate (39.3–48.9), or good (&gt;48.9). Physical function was assessed with the Short Physical Performance Battery (SPPB). Random effects tobit regression models were used to examine associations between dietary trajectories and later physical function.

Results
Two latent classes of AHEI scores were generated and labeled “greatly improved” or “moderately improved.” In the greatly improved class, participants showed a trend in overall AHEI score from poor/intermediate to good diet categories across dietary assessments with age, over time. In the moderately improved class, the overall AHEI score shifted from poor to intermediate diet categories over time, and the prevalence of the good diet category remained low. Mean AHEI score between ages 30 and 59 years was higher in the greatly, than moderately, improved class. The moderately improved class had 1.6 points lower SPPB score (indicating poorer physical function) at older age than the greatly improved class (p = .022).

Conclusions
Findings suggest that improving diet quality in middle age may contribute to better physical function in older age.
Heterochronic Parabiosis: Old Blood Induces Changes in Mitochondrial Structure and Function of Young Mice
Authors: Gonzalez-Armenta Jenny L , Li Ning , Lee Rae-Ling , Lu Baisong , Molina Anthony J A 
Published: December 2020

Abstract
Heterochronic parabiosis models have been utilized to demonstrate the role of blood-borne circulating factors in systemic effects of aging. In previous studies, heterochronic parabiosis has shown positive effects across multiple tissues in old mice. More recently, a study demonstrated old blood had a more profound negative effect on muscle performance and neurogenesis of young mice. In this study, we used heterochronic parabiosis to test the hypothesis that circulating factors mediate mitochondrial bioenergetic decline, a well-established biological hallmark of aging. We examined mitochondrial morphology, expression of mitochondrial complexes, and mitochondrial respiration from skeletal muscle of mice connected as heterochronic pairs, as well as young and old isochronic controls. Our results indicate that young heterochronic mice had significantly lower total mitochondrial content and on average had significantly smaller mitochondria compared to young isochronic controls. Expression of complex IV followed a similar pattern: young heterochronic mice had a trend for lower expression compared to young isochronic controls. Additionally, respirometric analyses indicate that young heterochronic mice had significantly lower complex I, complex I + II, and maximal mitochondrial respiration and a trend for lower complex II-driven respiration compared to young isochronic controls. Interestingly, we did not observe significant improvements in old heterochronic mice compared to old isochronic controls, demonstrating the profound deleterious effects of circulating factors from old mice on mitochondrial structure and function. We also found no significant differences between the young and old heterochronic mice, demonstrating that circulating factors can be a driver of age-related differences in mitochondrial structure and function.
GALLO: An R package for genomic annotation and integration of multiple data sources in livestock for positional candidate loci
Authors: Fonseca Pablo A S , Suárez-Vega Aroa , Marras Gabriele , Cánovas Ángela 
Published: December 2020

Abstract

Background
The development of high-throughput sequencing and genotyping methodologies has enabled the identification of thousands of genomic regions associated with several complex traits. The integration of multiple sources of biological information is a crucial step required to better understand patterns regulating the development of these traits.

Findings
Genomic Annotation in Livestock for positional candidate LOci (GALLO) is an R package developed for the accurate annotation of genes and quantitative trait loci (QTLs) located in regions identified in common genomic analyses performed in livestock, such as genome-wide association studies and transcriptomics using RNA sequencing. Moreover, GALLO allows the graphical visualization of gene and QTL annotation results, data comparison among different grouping factors (e.g., methods, breeds, tissues, statistical models, studies), and QTL enrichment in different livestock species such as cattle, pigs, sheep, and chickens.

Conclusions
Consequently, GALLO is a useful package for annotation, identification of hidden patterns across datasets, and data mining previously reported associations, as well as the efficient examination of the genetic architecture of complex traits in livestock.
Predicting pressure injury using nursing assessment phenotypes and machine learning methods
Authors: Song Wenyu , Kang Min-Jeoung , Zhang Linying , Jung Wonkyung , Song Jiyoun , Bates David W , Dykes Patricia C 
Published: December 2020

Abstract

Objective
Pressure injuries are common and serious complications for hospitalized patients. The pressure injury rate is an important patient safety metric and an indicator of the quality of nursing care. Timely and accurate prediction of pressure injury risk can significantly facilitate early prevention and treatment and avoid adverse outcomes. While many pressure injury risk assessment tools exist, most were developed before there was access to large clinical datasets and advanced statistical methods, limiting their accuracy. In this paper, we describe the development of machine learning-based predictive models, using phenotypes derived from nurse-entered direct patient assessment data.

Methods
We utilized rich electronic health record data, including full assessment records entered by nurses, from 5 different hospitals affiliated with a large integrated healthcare organization to develop machine learning-based prediction models for pressure injury. Five-fold cross-validation was conducted to evaluate model performance.

Results
Two pressure injury phenotypes were defined for model development: nonhospital acquired pressure injury (N = 4398) and hospital acquired pressure injury (N = 1767), representing 2 distinct clinical scenarios. A total of 28 clinical features were extracted and multiple machine learning predictive models were developed for both pressure injury phenotypes. The random forest model performed best and achieved an AUC of 0.92 and 0.94 in 2 test sets, respectively. The Glasgow coma scale, a nurse-entered level of consciousness measurement, was the most important feature for both groups.

Conclusions
This model accurately predicts pressure injury development and, if validated externally, may be helpful in widespread pressure injury prevention.
Estimates of Non-Alcoholic Food-Derived Ethanol and Methanol Exposure in Humans
Authors: Gürler Mukaddes , Martz Walter , Taştekin Burak , Najafova Tahmina , Dettmeyer Reinhard B 
Published: December 2020

Abstract
Food-derived alcohol is almost not in question due to its low concentration. Nevertheless, could it pose a problem for some risk groups and forensic cases? To answer this, we aimed to simultaneously evaluate ethanol and methanol ingredients of a variety of non-alcoholic foods in two different countries and estimate their possible health and forensic consequences. Alcohols in foods were analyzed by headspace gas chromatography. Human average acute daily food consumptions and food-derived blood alcohol concentrations (BACs) were determined by using the data of The European Food Safety Authority Nutrition Survey. Methanol and ethanol ingredients of similar foods varied between the two cities. Most foods produce higher methanol concentrations than the maximum allowable dose level (23 mg). Especially fruit juices lead to the critical level of ethanol for children (6 mg/kg body weight). Based on the results, adult daily intake of selected food groups does not bear ethanol that exceeds the legal limit of BAC or the limit not allowed from a religious perspective and does not lead to acute alcohol toxicity. But these low levels of ethanol and methanol consumed via non-alcoholic foods for life can raise the vulnerability to chronic health problems (cancer, liver cirrhosis, Alzheimer’s disease, autism, ocular toxicity and alterations in fetal development) and may lead to positive ethanol metabolite results (e.g., ethyl glucuronide) when a low cutoff level is used. Therefore, studies on the alcohol contents of various natural and processed non-alcoholic foods along with their effects on humans and new regulations on labeling the food products and conscious food consumption are of particular importance. It would also be important to consider unintentional alcohol consumption via non-alcoholic foods in the evaluation of clinical and forensic cases.
Fatal, Intentional Overdose of Ranolazine: Postmortem Distribution of Parent Drug and Its Major Metabolite
Authors: Gottardo Rossella , De Battisti Zeno , Federico Busetti , Murari Matilde , Tagliaro Franco 
Published: December 2020

Abstract
Ranolazine is a selective inhibitor of the late inward sodium current, approved for the treatment of chronic angina. Here, a case of a possibly suicidal death due to acute ranolazine overdosing is reported. A 41-year-old woman was found unconscious by her son and was urgently admitted to the intensive care unit. She had ingested an unknown amount of ranolazine tablets. Seventeen hours after admission, the patient died. An autopsy was performed 4 days postmortem. A routine screening analysis for drugs of abuse and medicinal drugs performed by liquid chromatography (LC)–ion trap mass spectrometry (MS) on autopsy samples of biological fluids did not detect any relevant presence of toxicologically relevant compounds, but ranolazine. A quantitative analysis was then carried out by liquid chromatography--triple quadrupole (LC–QqQ) MS in order to quantify ranolazine and its major metabolite o-desmethylranolazine in biological fluids and organs. Ranolazine concentrations in biological fluids were as follows: cardiac blood, 19.5 μg/mL; femoral blood, 12.3 μg/mL; bile, 0.87 μg/mL and vitreous humor, 15.4 μg/mL. For O-desmethylranolazine, the concentrations in cardiac blood, femoral blood, bile and vitreous humor were 10.7, 9.6, 11,103 and 11.4 μg/mL, respectively. The cause of death was attributed to ranolazine overdosing. To the best of our knowledge, this is the first report of a fatality associated with ranolazine, in which the postmortem distribution of ranolazine and its metabolite has been quantitatively assessed. The present study can therefore provide useful information for interpretation of the causes and mechanisms of death in ranolazine-associated fatalities.