bluuebunny/crossref_metadata_2025_split huggingface dataset:
Retracted: JMJD6‐STAT3Y705ph axis promotes autophagy in osteosarcoma cancer cells by regulating ATG
Authors: Wang Kun , Du Baoyin , Xu Bin , Lv Xin 
Published: January 2020

AbstractBackgroundOsteosarcoma cancer is a malignant tumor with poor outcome. Activation of STAT3 is closely related with tumor development. We intended to study the effects of JMJD6 on phosphorylation of STAT3 at Y705 site.MethodsOsteosarcoma cancer cell lines (Saos‐2, MG‐63, and HOS) and clinical specimens were obtained. Interference RNA or JMJD6 mimic was transfected into the cells to silence or mimic JMJD6. Immunoprecipitation assay and glutathione S‐transferase (GST) pull down was implemented to investigate whether JMJD6 is associated with STAT3. STAT3‐null HOS cells were simultaneously transfected with the plasmids bearing STAT3WT or STAT3Y705F and EGFP‐tagged LC3 plasmids. Recombinant full‐length JMJD6 protein was subjected to in vitro kinase activity assay for testing its ability to phosphorylate STAT3. The severity of autophagy was indicated by the number of autophagosomes, expression of EGFP‐LC3, ratio of LC3‐II to LC3‐I, degradation percentage of long‐lived proteins and expression of autophagy associated gene (ATG).ResultsJMJD6 modulated the phosphorylation of STAT3 at Y705 site in osteosarcoma cells. Results from immunoprecipitation and GST pull down assays showed that JMJD6 associated with STAT3 in osteosarcoma cells. JMJD6 silence impeded the formation of autophagosomes, inhibited the accumulation of EGFP‐LC3, decreased the ratio of LC3‐II to LC3‐I, blocked the degradation of long‐lived proteins, and repressed the expression of ATG. JMJD6‐induced autophagy was impaired by STAT3Y705F which was not phosphorylated by JMJD6. The JMJD6‐STAT3Y705ph axis was implicated in the transcriptional regulation of ATG.ConclusionJMJD6 was included in regulating the phosphorylation of STAT3Y705 and promoted autophagy of osteosarcoma cells through its kinase activity.
Three‐Dimensional (3D) cell culture monitoring: Opportunities and challenges for impedance spectroscopy
Authors: De León Sorel E. , Pupovac Aleta , McArthur Sally L. 
Published: January 2020

AbstractThree‐dimensional (3D) cell culture has developed rapidly over the past 5–10 years with the goal of better replicating human physiology and tissue complexity in the laboratory. Quantifying cellular responses is fundamental in understanding how cells and tissues respond during their growth cycle and in response to external stimuli. There is a need to develop and validate tools that can give insight into cell number, viability, and distribution in real‐time, nondestructively and without the use of stains or other labelling processes. Impedance spectroscopy can address all of these challenges and is currently used both commercially and in academic laboratories to measure cellular processes in 2D cell culture systems. However, its use in 3D cultures is not straight forward due to the complexity of the electrical circuit model of 3D tissues. In addition, there are challenges in the design and integration of electrodes within 3D cell culture systems. Researchers have used a range of strategies to implement impedance spectroscopy in 3D systems. This review examines electrode design, integration, and outcomes of a range of impedance spectroscopy studies and multiparametric systems relevant to 3D cell cultures. While these systems provide whole culture data, impedance tomography approaches have shown how this technique can be used to achieve spatial resolution. This review demonstrates how impedance spectroscopy and tomography can be used to provide real‐time sensing in 3D cell cultures, but challenges remain in integrating electrodes without affecting cell culture functionality. If these challenges can be addressed and more realistic electrical models for 3D tissues developed, the implementation of impedance‐based systems will be able to provide real‐time, quantitative tracking of 3D cell culture systems.
Plant‐derived protein bodies as delivery vehicles for recombinant proteins into mammalian cells
Authors: Schwestka Jennifer , Tschofen Marc , Vogt Stefan , Marcel Sylvain , Grillari Johannes , Raith Marianne , Swoboda Ines , Stoger Eva 
Published: January 2020

AbstractThe encapsulation of biopharmaceuticals into micro‐ or nanoparticles is a strategy frequently used to prevent degradation or to achieve the slow release of therapeutics and vaccines. Protein bodies (PBs), which occur naturally as storage organelles in seeds, can be used as such carrier vehicles. The fusion of the N‐terminal sequence of the maize storage protein, γ‐zein, to other proteins is sufficient to induce the formation of PBs, which can be used to bioencapsulate recombinant proteins directly in the plant production host. In addition, the immunostimulatory effects of zein have been reported, which are advantageous for vaccine delivery. However, little is known about the interaction between zein PBs and mammalian cells. To better understand this interaction, fluorescent PBs, resulting from the fusion of the N‐terminal portion of zein to a green fluorescent protein, was produced in Nicotiana benthamiana leaves, recovered by a filtration‐based downstream procedure, and used to investigate their internalization efficiency into mammalian cells. We show that fluorescent PBs were efficiently internalized into intestinal epithelial cells and antigen‐presenting cells (APCs) at a higher rate than polystyrene beads of comparable size. Furthermore, we observed that PBs stimulated cytokine secretion by epithelial cells, a characteristic that may confer vaccine adjuvant activities through the recruitment of APCs. Taken together, these results support the use of zein fusion proteins in developing novel approaches for drug delivery based on controlled protein packaging into plant PBs.
Online control of cell culture redox potential prevents antibody interchain disulfide bond reduction
Authors: Handlogten Michael W. , Wang Jihong , Ahuja Sanjeev 
Published: January 2020

AbstractThe phenomenon of monoclonal antibody (mAb) interchain disulfide bond reduction during manufacturing processes continues to be a focus of the biotechnology industry due to the potential for loss of product, increased complexity of purification processes, and reduced stability of the drug product. We hypothesized that antibody reduction can be mitigated by controlling the cell culture redox potential and subsequently established a threshold redox potential above which the mAb remained intact and below which there were significant and highly variable amounts of reduced mAb. Using this knowledge, we developed three control schemes to prevent mAb reduction in the bioreactor by controlling the cell culture redox potential via an online redox probe. These control methodologies functioned by increasing the concentration of dissolved oxygen (DO), copper (II) (Cu), or both DO and Cu to maintain the redox potential above the threshold value. Using these methods, we were able to demonstrate successful control of antibody reduction. Importantly, the redox control strategies did not significantly impact the cell growth, viability, mAb production, or product quality attributes including aggregates, C‐terminal lysine, high mannose, deamidation, and glycation. Our results demonstrate that controlling the cell culture redox potential is a simple and effective method to prevent mAb reduction.
Pressurized intraperitoneal aerosol chemotherapy: a review of the introduction of a new surgical technology using the IDEAL framework
Authors: Tate S J , Torkington J 
Published: January 2020

Abstract

Background
The IDEAL (Idea, Development, Evaluation, Assessment, Long-term study) framework is a scheme of investigation for innovative surgical therapeutic interventions. Pressurized intraperitoneal aerosol chemotherapy (PIPAC) is a procedure based on laparoscopy to deliver intraperitoneal chemotherapy for peritoneal metastases, introduced in 2011. The aim of this article was to review literature on PIPAC and assess whether development of the technique has followed the IDEAL framework.

Methods
A search of MEDLINE and Embase was carried out to identify scientific reports on PIPAC published between January 2000 and February 2019. The studies were categorized according to the IDEAL stages.

Results
Eighty-six original research papers on PIPAC were identified. There were 23 stage 0, 18 stage 1, 25 stage 2a and six stage 2b studies. Protocol papers for stage 1, 2b and 3 studies, and trial registrations for stage 2a studies, were also identified. The number of centres publishing reports and the number of publications has increased each year. Overall, there has been progression through the IDEAL stages; however, about 60 per cent of clinical reports published in 2018 were stage 1 Idea-type studies.

Conclusion
Since its introduction, studies investigating PIPAC have progressed in line with the IDEAL framework. However, the majority of studies reported recently were stage 0 and 1 studies.
A trip down memory lane: Antecedents and outcomes of ad‐evoked nostalgia on Facebook
Authors: Youn Seounmi 
Published: January 2020

AbstractIntegrating the theories of nostalgia and consumer‐brand relationships, this study developed and tested a theoretical model for understanding ad‐evoked nostalgia within the context of Facebook. Online survey data (n = 395) were collected using Amazon MTurk to disentangle the relationships between antecedents, ad‐evoked nostalgia, and outcomes. An analysis of the structural model revealed that the need to belong and nostalgia proneness had positive, direct effects on ad‐evoked nostalgia, which subsequently affected self‐brand connections and brand engagement behaviors on Facebook. Facebook use intensity was not found to influence ad‐evoked nostalgia. Theoretical and managerial implications, along with future research directions, were discussed.
Percutaneous closure of patent ductus arteriosus in premature infants: A French national survey: A Titanic movement in technology and technique— Beware the icebergs!
Authors: Wilson Neil 
Published: January 2020

Key Points
First national multicenter report of interventional closure of patent ductus arteriosus in preterm infants.
Results describe commendable technical and procedural success of 99% with year‐on‐year tendency for intervention in smaller patients.
Reported complications (8.9%) and outcome (1‐year mortality 6.8%) are far superior to contemporary surgical intervention but indicate room for improvement.
Communication to cure cath chaos
Authors: Blankenship James C. 
Published: January 2020

Key Points
Communication errors in the cath lab may lead to incorrect medication administration or dosing, or incorrect sizes of devices deployed in patients, with catastrophic results. Use of closed‐loop communication minimizes these errors.
In this study, two rounds of quality improvement projects improved, but did not completely close the communication gap.
Standard setting by professional societies and individual cath lab leaders may be necessary to improve the use of closed‐loop communication in cath labs—although a completely successful strategy remains to be identified.
Microfluidic System with Extended‐Gate‐Type Organic Transistor for Real‐Time Glucose Monitoring
Authors: Didier Pierre , Lobato‐Dauzier Nicolas , Clément Nicolas , Genot Anthony J. , Sasaki Yui , Leclerc Éric , Minamiki Tsukuru , Sakai Yasuyuki , Fujii Teruo , Minami Tsuyoshi 
Published: January 2020

AbstractOrganic field‐effect transistors (OFETs) can be potentially employed to monitor cell activities for healthcare and medical treatment because of their attractive properties such as ease of use, flexibility, and low‐cost manufacturing processes. Although current OFET‐based sensors are suitable for point‐of‐care testing, the establishment of real‐time monitoring methods is in high demand for continuous monitoring of health conditions and/or biological cell activities. In this regard, we herein propose a microfluidic platform integrated with an extended‐gate‐type OFET for real‐time glucose monitoring. The mechanism of glucose detection depends on the artificial receptor phenylboronic acid and its boronate esterification. After optimization of the microfluidics for the OFET‐based sensor, the sensor was used to monitor glucose consumption and release in a model of pseudo‐liver cells. Random increases or decreases in the glucose concentration were reproducibly monitored.
Electrochemical Reduction of CO2 into C1 and C2 Hydrocarbons Using Dendritic Cu and Cu2O Electrodes
Authors: Feroze Muhammad Tajmeel , Sami Syed Kamran , Doonyapisut Dulyawat , Kim Byeongkyu , Chung Chan‐Hwa 
Published: January 2020

AbstractElectroreduction of carbon dioxide to C1 and C2 hydrocarbons has emerged as an efficient way to utilize carbon dioxide and maintain the broken carbon‐cycle in the environment. Copper is a promising catalyst for such a purpose. The selectivity and stability of hydrocarbon production is still a challenge. Here we propose a simple one‐step electro‐deposition of copper followed by annealing in air, in which the dendritic structures of Cu and Cu2O electrodes have been prepared, to enhance the copper‐electrode properties in terms of morphology, selectivity, stability, and electrochemical performance. The selectivity of ethylene using modified dendritic Cu2O electrodes remains stable (from 19.21 % to 18.01 %) after the course of over 10 hours by applying a constant potential of −1.4 V. Our finding suggests a promising lead for the design of practical catalytic electrodes for the fuel synthesis with electrochemical CO2 reduction.
Influence of Quantum Dot Surface on Electrochemical DNA Sensing Mechanism
Authors: Fuku Xolile , Baker Priscilla , Iwuoha Emmanuel 
Published: January 2020

AbstractOwing to their high surface‐to‐volume ratio, electrocatalytic activity, biocompatibility and novel electron transport properties, quantum dots (QDs) are highly attractive materials for the ultrasensitive detection of biological macromolecules via bioelectronic devices. In this study, a QD‐based genosensor was developed, in which Ga2Te3‐based QDs were synthesized using an aqueous solution approach by mixing 3‐mercaptosuccinic acid (3MSA)‐capped gallium metal precursor with reduced tellurium metal. The results enabled us to reach an original understanding related to the active material involved in the probe DNA sensing mechanism. The morphological and structural characterization of the QDs was performed prior to their utilization in a DNA sensor construction. High‐resolution TEM (HR‐TEM) and atomic force microscopy (AFM) images confirmed the spherical and crystalline nature of the QDs, whereas X‐ray photoelectron spectroscopy (XPS) and X‐ray diffraction (XRD) analyses were able to confirm the oxidation states and formation of the prepared QDs. UV/Vis was capable of finding the optical band gap energy and the photostability of the QDs. The resultant Ga2Te3 QDs together with metal ions confirmed their use for DNA signal detection through their DNA binding mechanism in the genosensor construction. Genosensing in Cs+ and Li+ ions exhibited high sensitivity (2.74–3.69 μA ng−1 mL) and very low detection limits (0.4 pg mL−1) with a linear dynamic range of 0.1–1 ng mL−1.
Multi‐Spectroscopic Interrogation of the Spatial Linker Distribution in Defect‐Engineered Metal–Organic Framework Crystals: The [Cu3(btc)2−x(cydc)x] Showcase
Authors: Rivera‐Torrente Miguel , Filez Matthias , Meirer Florian , Weckhuysen Bert M. 
Published: January 2020

AbstractIn the past few years, defect‐engineered metal–organic frameworks (DEMOFs) have been studied due to the plethora of textural, catalytic, or magnetic properties that can be enhanced by carefully introducing defect sites into the crystal lattices of MOFs. In this work, the spatial distribution of two different non‐defective and defective linkers, namely 1,3,5‐benzenetricarboxylate (BTC) and 5‐cyano‐1,3‐benzenedicarboxylate (CYDC), respectively, has been studied in different DEMOF crystals of the HKUST‐1 topology. Raman micro‐spectroscopy revealed a nonhomogeneous distribution of defect sites within the [Cu3(btc)2−x(cydc)x] crystals, with the CYDC linker incorporated into defect‐rich or defect‐free areas of selected crystals. Additionally, advanced bulk techniques have shed light on the nature of the copper species, which is highly dynamic and directly affects the reactivity of the copper sites, as shown by probe molecule FTIR spectroscopy. Furthermore, electron microscopy revealed the effect of co‐crystallizing CYDC and BTC on the crystal size and the formation of mesopores, further corroborated by X‐ray scattering analysis. In this way we have demonstrated the necessity of utilizing micro‐spectroscopy along with a whole array of bulk spectroscopic techniques to fully describe multicomponent metal–organic frameworks.
Diphenylberyllium Reinvestigated: Structure, Properties, and Reactivity of BePh2, [(12‐crown‐4)BePh]+, and [BePh3]−
Authors: Müller Matthias , Buchner Magnus R. 
Published: January 2020

AbstractThe first synthesis of BePh2 was accomplished almost a century ago. However, its structure has remained unknown so far, while the corresponding aryls of the elements adjacent to beryllium in the periodic table are well investigated. Herein, we present an improved synthesis for diphenylberyllium and show by X‐ray diffraction that it forms a trinuclear complex in the solid state. NMR spectroscopy revealed that this structure is also retained in solution but exhibits dynamic behavior. Its stability against heat and coordinating solvents is discussed and the possible obstacles to the synthesis of BePh2 from BeCl2 are examined. In the process of this study two ether adducts, BePh2⋅Et2O and Be2Ph4⋅Et2O, have been characterized as well as the previously unknown triphenylberyllate anion. From the latter several single‐crystal structures were obtained under various conditions, in which [BePh3]− is either isolated or acts as a ligand for Li+. Furthermore, the crown ether induced selfionization of BePh2 is described and the resulting [(12‐crown‐4)BePh]+ cation was isolated, which shows an unusual 4+1 coordination around the Be atom.
6‐Triphenylphosphinehexanoic Acid Conjugated Near‐Infrared Persistent Luminescence Nanoprobe for Autofluorescence‐Free Targeted Imaging of Mitochondria in Cancer Cells
Authors: Su Yu‐Bin , Zhao Xu , Chen Li‐Jian , Qian Hai‐Long , Yan Xiu‐Ping 
Published: January 2020

AbstractMitochondria, as the energy provider for cells, play a key role in cell physiological processes. Mitochondrion‐targeted imaging is of great significance for understanding the physiological functions and pathology involved in various diseases. Herein, we report the fabrication of 6‐triphenylphosphinehexanoic acid (TPP) conjugated near‐infrared persistent luminescence nanoparticles (PLNP) (PLNP‐TPP) for mitochondria‐targeted imaging in cancer cells. The PLNP was prepared by doping Cr(III) into gallogermanate through a hydrothermal method. The as‐prepared PLNP showed small size, monodispersity, excellent near‐infrared (NIR) luminescence and visible light renewable NIR luminescence. The grafted TPP facilitated the transportation of PLNP‐TPP across the mitochondrial membrane and enabled staining of the mitochondrial region in live cancer cells. This work provides a promising strategy for fabricating PLNP nanoprobes to realize mitochondrial‐targeting bioimaging.
Synthetic Biology Enables Programmable Cell‐Based Biosensors
Authors: Hicks Maggie , Bachmann Till T. , Wang Baojun 
Published: January 2020

AbstractThe front cover artwork is provided by the group of Dr. Baojun Wang (The University of Edinburgh). The image shows an engineered bacterial cell containing a genetic amplifier circuit which transforms a weak input signal into a larger easily detectable output signal. The electronics symbols used to illustrate the genetic circuit highlight the programmability of the circuit components enabled by state‐of‐the‐art synthetic biology tools. Read the full text of the Review at 10.1002/cphc.201900739.
Real‐time performance assessment using fast interrupt request on a standard Linux kernel
Authors: Souza Rayanne , Freitas Miguel , Jimenez Marcelo , Magalhães João , Kubrusly Alan C. , Rodriguez Noemi 
Published: January 2020

SummaryThis article presents the use of ARM's fast interrupt request (FIQ) to accomplish better jitter performance on real‐time drivers without using patches for real‐time extensions on the native Linux kernel code. Writing an FIQ interrupt handler is challenging due to the lack of Linux kernel support and the need to avoid page faults exception during its execution. We investigate and evaluate a mechanism that employs static mapping for peripherals and changes on the Linux kernel code to allow the FIQ interrupt handler to be written in the C language. Furthermore, the FIQ performance was evaluated by comparing it with a timer Interrupt Request on Linux PREEMPT‐RT in full CONFIG_PREEMPT_RT mode. Both were applied on a Linux driver for data acquisition of a pipeline inspection gauge system. Results show that the FIQ approach was able to reduce in 97.49% the interrupt jitter and, as a result, allowed an increase in the data acquisition frequency from 1024 Hz to 2048 Hz, showing that the FIQ approach can be considered for real‐time applications without resorting to real‐time extensions.
Correcting the January optimism effect
Authors: Franses Philip Hans 
Published: January 2020

AbstractEach month, various professional forecasters give forecasts for next year's real gross domestic product (GDP) growth and unemployment. January is a special month, when the forecast horizon moves to the following calendar year. Instead of deleting the January data when analyzing forecast updates, I propose a periodic version of a test regression for weak‐form efficiency. An application of this periodic model for many forecasts across a range of countries shows that in January GDP forecast updates are positive, whereas the forecast updates for unemployment are negative. I document that this January optimism about the new calendar year is detrimental to forecast accuracy. To empirically analyze Okun's law, I also propose a periodic test regression, and its application provides more support for this law.
Genetic effect estimates in case‐control studies when a continuous variable is omitted from the model
Authors: Sheng Ying , Huang Chiung‐Yu , Lobach Siarhei , Zablotska Lydia , Lobach Iryna 
Published: January 2020

AbstractLarge‐scale genome‐wide analyses scans on massive numbers of various cases and controls are archived in the genetic databases that are publically available, for example, the Database of Genotypes and Phenotypes (https://www.ncbi.nlm.nih.gov/gap/). These databases offer unprecscendented opportunity to study the genetic effects. Yet, the set of nongenetic variables in these databases is often brief. From the statistical literature, we know that omitting a continuous variable from a logistic regression model can result in biased estimates of odds ratios (OR), even when the omitted and the included variables are independent. We are interested in assessing what information is needed to recover the bias in the OR estimate of genotype due to omitting a continuous variable in settings when the actual values of the omitted variable are not available. We derive two estimating procedures that can recover the degree of bias based on a conditional density of the omitted variable given the disease status and the genotype or the known distribution of the omitted variable and frequency of the disease in the population. Importantly, our derivations show that omitting a continuous variable can result in either under‐ or over‐estimation of the genetic effects. We performed extensive simulation studies to examine bias, variability, false‐positive rate, and power in the model that omits a continuous variable. We show the application to two genome‐wide studies of Alzheimer's disease.