bluuebunny/crossref_metadata_2025_split huggingface dataset:
Inflammation as a predictor of acute kidney injury and mediator of higher mortality after acute kidney injury in non-cardiac surgery
Authors: Murashima Miho , Nishimoto Masatoshi , Kokubu Maiko , Hamano Takayuki , Matsui Masaru , Eriguchi Masahiro , Samejima Ken-ichi , Akai Yasuhiro , Tsuruya Kazuhiko 
Published: December 2019

AbstractThis retrospective cohort study examined the roles of inflammation in acute kidney injury (AKI). Serum albumin and C-reactive protein (CRP) were used as markers of inflammation. Adults who underwent non–cardiac surgery from 2007 to 2011 were included. Exclusion criteria were urological surgery, obstetric surgery, missing data, and pre-operative dialysis. Subjects were followed until the end of 2015 or loss to follow-up. Associations between pre–operative albumin or CRP and post-operative AKI or association between AKI and mortality were examined by logistic or Cox regression, respectively. Mediation analyses were performed using albumin and CRP as mediators. Among 4,538 subjects, 272 developed AKI. Pre-operative albumin was independently associated with AKI (odds ratio [95% confidence interval (CI)]: 0.63 [0.48–0.83]). During a median follow-up of 4.5 years, 649 died. AKI was significantly associated with mortality (hazard ratio [HR] [95% CI]: 1.58 [1.22–2.04]). Further adjustment for pre-operative albumin and CRP attenuated the association (HR [95% CI]: 1.28 [0.99–1.67]). The proportions explained by mediating effects of lnCRP and albumin were 29.3% and 39.2% and mediation effects were statistically significant. In conclusion, inflammation is a predictor of AKI and a mediator of mortality after AKI. Interventions targeting inflammation might improve outcomes of AKI.
Gestational exposure to the cannabinoid WIN 55,212-2 and its effect on the innate intestinal immune response
Authors: Hernández-Cervantes Rosalía , Pérez-Torres Armando , Prospéro-García Óscar , Morales Montor Jorge 
Published: December 2019

AbstractThe consequences of marijuana consumption during pregnancy and its effects on the function of the immune system have been little studied. Marijuana is one of the most consumed recreational drugs among pregnant women, and it is known that gestational exposure to marijuana can have serious effects on the offspring after birth. In this study, we challenged the immune system of Wistar rats by infecting them with the parasitic nematode Trichinella spiralis. A treatment group of these animals was prenatally exposed to the cannabinoid WIN 55,212-2; a control group was not exposed. At 5 days of infection, the treated animals were less effective in eliminating intestinal parasites; moreover, this effect was correlated with a deficiency in mucus production, lower recruitment of eosinophils in the duodenum, and a reduced percentage of Tγδ and NK cells. In conclusion, the gestational administration of the synthetic cannabinoid WIN 55,212-2 induces lasting changes to the function of the immune system against infection with T. spiralis in male Wistar rats, making them more susceptible to infection.
Hyperuniform disordered waveguides and devices for near infrared silicon photonics
Authors: Milošević Milan M. , Man Weining , Nahal Geev , Steinhardt Paul J. , Torquato Salvatore , Chaikin Paul M. , Amoah Timothy , Yu Bowen , Mullen Ruth Ann , Florescu Marian 
Published: December 2019

AbstractWe introduce a hyperuniform-disordered platform for the realization of near-infrared photonic devices on a silicon-on-insulator platform, demonstrating the functionality of these structures in a flexible silicon photonics integrated circuit platform unconstrained by crystalline symmetries. The designs proposed advantageously leverage the large, complete, and isotropic photonic band gaps provided by hyperuniform disordered structures. An integrated design for a compact, sub-volt, sub-fJ/bit, hyperuniform-clad, electrically controlled resonant optical modulator suitable for fabrication in the silicon photonics ecosystem is presented along with simulation results. We also report results for passive device elements, including waveguides and resonators, which are seamlessly integrated with conventional silicon-on-insulator strip waveguides and vertical couplers. We show that the hyperuniform-disordered platform enables improved compactness, enhanced energy efficiency, and better temperature stability compared to the silicon photonics devices based on rib and strip waveguides.
Exogenous and endogenous microbiomes of wild-caught Phormia regina (Diptera: Calliphoridae) flies from a suburban farm by 16S rRNA gene sequencing
Authors: Deguenon Jean M. , Travanty Nicholas , Zhu Jiwei , Carr Ann , Denning Steven , Reiskind Michael H. , Watson David W. , Michael Roe R. , Ponnusamy Loganathan 
Published: December 2019

AbstractThe black blow fly,Phormia regina(Meigen) (Diptera: Calliphoridae) is one of the most abundant carrion flies in North America. Calliphorids are important in agriculture and animal production, veterinary sciences, forensics and medical entomology. While the role of flies in the epidemiology of human and animal diseases is an active area of research, little is known about the microorganisms associated with these insects. We examined the diversity of wild-caught black blow fly endogenous (internal body) and exogenous (external body) microbial communities using 16S rRNA gene sequencing. Overall, 27 phyla, 171 families and 533 genera were detected, and diversity was significantly higher (P< 0.05) on external body surfaces. At the genus level,Dysgonomonas,Ignatzschineria,Acinetobacter,Vagococcus,Myroides, andWohlfahrtiimonaswere predominant. Cloning and sequencing of nearly full-length fragments of the 16S rRNA gene showed that some of the species identified are known to be pathogenic to humans, animals, and plants.Myroides odoratimimusandAcinetobacter radioresistensare well-known, multi-drug resistant bacteria. These results provide a snapshot of the microbial communities harbored by adult black blow flies and call for more comprehensive studies to better characterize the role these flies may play in the transmission of pathogenic microorganisms.
Prediction of IDH and TERT promoter mutations in low-grade glioma from magnetic resonance images using a convolutional neural network
Authors: Fukuma Ryohei , Yanagisawa Takufumi , Kinoshita Manabu , Shinozaki Takashi , Arita Hideyuki , Kawaguchi Atsushi , Takahashi Masamichi , Narita Yoshitaka , Terakawa Yuzo , Tsuyuguchi Naohiro , Okita Yoshiko , Nonaka Masahiro , Moriuchi Shusuke , Takagaki Masatoshi , Fujimoto Yasunori , Fukai Junya , Izumoto Shuichi , Ishibashi Kenichi , Nakajima Yoshikazu , Shofuda Tomoko , Kanematsu Daisuke , Yoshioka Ema , Kodama Yoshinori , Mano Masayuki , Mori Kanji , Ichimura Koichi , Kanemura Yonehiro , Kishima Haruhiko 
Published: December 2019

AbstractIdentification of genotypes is crucial for treatment of glioma. Here, we developed a method to predict tumor genotypes using a pretrained convolutional neural network (CNN) from magnetic resonance (MR) images and compared the accuracy to that of a diagnosis based on conventional radiomic features and patient age. Multisite preoperative MR images of 164 patients with grade II/III glioma were grouped by IDH and TERT promoter (pTERT) mutations as follows: (1) IDH wild type, (2) IDH and pTERT co-mutations, (3) IDH mutant and pTERT wild type. We applied a CNN (AlexNet) to four types of MR sequence and obtained the CNN texture features to classify the groups with a linear support vector machine. The classification was also performed using conventional radiomic features and/or patient age. Using all features, we succeeded in classifying patients with an accuracy of 63.1%, which was significantly higher than the accuracy obtained from using either the radiomic features or patient age alone. In particular, prediction of the pTERT mutation was significantly improved by the CNN texture features. In conclusion, the pretrained CNN texture features capture the information of IDH and TERT genotypes in grade II/III gliomas better than the conventional radiomic features.
Oral administration of EP4-selective agonist KAG-308 suppresses mouse knee osteoarthritis development through reduction of chondrocyte hypertrophy and TNF secretion
Authors: Murahashi Yasutaka , Yano Fumiko , Chijimatsu Ryota , Nakamoto Hideki , Maenohara Yuji , Amakawa Masahiro , Miyake Yoshihide , Yamanaka Hiroyuki , Iba Kousuke , Yamashita Toshihiko , Tanaka Sakae , Saito Taku 
Published: December 2019

AbstractOsteoarthritis (OA) is one of the world’s most common degenerative diseases, but there is no disease-modifying treatment available. Previous studies have shown that prostaglandin E2 (PGE2) and PGE2 receptor 4 (EP4) are involved in OA pathogenesis; however, their roles are not fully understood. Here, we examined the efficacy of oral administration of KAG-308, an EP4-selective agonist, in surgically induced mouse knee OA. Cartilage degeneration and synovitis were significantly inhibited by the KAG-308 treatment. Chondrocyte hypertrophy and expression of tumor necrosis factor alpha (TNF) and matrix metalloproteinase 13 (Mmp13) in the synovium were suppressed in the KAG-308-treated mice. In cultured chondrocytes, hypertrophic differentiation was inhibited by KAG-308 and intranuclear translocation of histone deacetylase 4 (Hdac4) was enhanced. In cultured synoviocytes, lipopolysaccharide (LPS)-induced expression of TNF and Mmp13 was also suppressed by KAG-308. KAG-308 was detected in the synovium and cartilage of orally treated mice. TNF secretion from the synovia of KAG-308-treated mice was significantly lower than control mice. Thus, we conclude that oral administration of KAG-308 suppresses OA development through suppression of chondrocyte hypertrophy and synovitis. KAG-308 may be a potent candidate for OA drug development.
Effects of miR-98 in intrauterine extracellular vesicles on maternal immune regulation during the peri-implantation period in cattle
Authors: Nakamura Keigo , Kusama Kazuya , Ideta Atsushi , Kimura Koji , Hori Masatoshi , Imakawa Kazuhiko 
Published: December 2019

AbstractEvidence accumulated suggests that extracellular vesicles (EVs) present in uterine lumen play a role in conceptus-endometrial cell interactions during peri-implantation periods. However, how intrauterine EVs function on endometrium have not been well characterized. To study how intrauterine EVs affect endometrial milieu in cattle, bovine endometrial epithelial cells (EECs) were treated with EVs isolated from uterine flushing fluids (UFs) on day 17 or 20 pregnancy (P17, P20, respectively; conceptus implantation to endometrium begins on days 19–19.5). RNA extracted from EECs were then subjected to RNA sequence analysis. The analysis revealed that transcripts related to immune system were down-regulated in EECs treated with EVs on P20 compared with those on P17. To investigate whether microRNAs (miRNAs) in EVs regulate maternal immune system in the endometrium during the peri-implantation, microRNA sequence and in silico analyses were performed, identifying bta-miR-98 in EVs as a potential miRNA to regulate maternal immune system. Furthermore, the treatment of EECs with bta-miR-98 negatively regulated several immune system-related genes, CTSC, IL6, CASP4 and IKBKE, in EECs. These results suggest that EVs containing bta-miR-98 is a regulator of maternal immune system, possibly allowing the conceptus attachment to the endometrial epithelium during the peri-implantation period.
Adaptation of muscle activation after patellar loading demonstrates neural control of joint variables
Authors: Barroso Filipe O. , Alessandro Cristiano , Tresch Matthew C. 
Published: December 2019

AbstractWe evaluated whether the central nervous system (CNS) chooses muscle activations not only to achieve behavioral goals but also to minimize stresses and strains within joints. We analyzed the coordination between quadriceps muscles during locomotion in rats before and after imposing a lateral force on the patella. Vastus lateralis (VL) and vastus medialis (VM) in the rat produce identical knee torques but opposing mediolateral patellar forces. If the CNS regulates internal joint stresses, we predicted that after imposing a lateral patellar load by attaching a spring between the patella and lateral femur, the CNS would reduce the ratio between VL and VM activation to minimize net mediolateral patellar forces. Our results confirmed this prediction, showing that VL activation was reduced after attaching the spring whereas VM and rectus femoris (RF) activations were not significantly changed. This adaptation was reversed after the spring was detached. These changes were not observed immediately after attaching the spring but only developed after 3–5 days, suggesting that they reflected gradual processes rather than immediate compensatory reflexes. Overall, these results support the hypothesis that the CNS chooses muscle activations to regulate internal joint variables.
Anti-escaping of incident laser in rare-earth doped fluoride ceramics with glass forming layer
Authors: Shi H. F. , Lin P. J. , Yang J. X. , Yuan J. L. , Pun E. Y. B. , Song Y. , Zhao X. , Lin H. 
Published: December 2019

AbstractAdaptive fluoride ceramic with glass forming layer (GCZBL-Er) used in laser anti-escaping has been prepared by one-step synthesis, and the thickness of glass layer is identified as ~0.41 mm. Blue, green and red emissions of Er3+/Yb3+ codoped fluoride ceramic (CZBL-Er) and glass layer (GZBL-Er) have been investigated under ~980 nm laser pumping. With the forming of thin glass layer on ceramic surface, the absorption intensities on diffuse reflection of GCZBL-Er at 974 nm and 1.53 μm increase by 48% and 53% than those of CZBL-Er. Excited by a 979 nm laser, the presence of the glass layer increases the absolute absorption rate in spectral power from 75% in CZBL-Er to 83% in GCZBL-Er, which is consistent with the improvement in the absorbed photon number. In addition, the quantum yield of GCZBL-Er complex is raised by 28.4% compared to the case of ceramic substrate by photon quantification. Intense absorption-conversion ability and efficient macroscopical anti-escaping effect confirm the superiority of ingenious structure in the fluoride ceramics with glass forming layer, which provides a new approach for developing the absorption-conversion materials of anti-NIR laser detection.
In Utero Exposure to Glucocorticoids and Pubertal Timing in Sons and Daughters
Authors: Sand Sofie Aagaard , Ernst Andreas , Lunddorf Lea Lykke Harrits , Brix Nis , Gaml-Sørensen Anne , Ramlau-Hansen Cecilia Høst 
Published: December 2019

AbstractEarly pubertal timing has been associated with adult diseases, and identifying preventable causes is of importance. In utero exposure to exogenous glucocorticoids, has been associated with changes in the reproductive hormonal axes in the children, which may influence pubertal timing. Exogenous glucocorticoids can be indicated for diseases such as asthma, allergy, skin diseases, as well as muscle and joint diseases. The aim was to explore the association between in utero exposure to glucocorticoids and pubertal timing in the children. This population-based study was conducted in the Puberty Cohort including 15,819 children, which is a sub-cohort of the Danish National Birth Cohort. Information on maternal glucocorticoid treatment was collected through interviews during pregnancy. Information on pubertal timing was obtained by questionnaires every 6 months throughout puberty, including Tanner Stages, axillary hair, acne, voice break, first ejaculation and menarche. The potential impact of confounding by indication was explored by stratifying on indication and treatment status. Overall, 6.8% of the children were exposed to glucocorticoids in utero. Exposure to glucocorticoids in utero was not associated with earlier puberty for neither boys nor girls with combined estimates of 0.4 months (95% CI: –1.5; 2.2) and –0.7 months (95% CI: –2.5; 1.2).
The Relationship Between Evaluation Methods for Chemotherapy-Induced Peripheral Neuropathy
Authors: Yoshida Yoichiro , Satoh Atsushi , Yamada Teppei , Aisu Naoya , Matsuoka Taisuke , Koganemaru Tomoko , Kajitani Ryuji , Munechika Taro , Matsumoto Yoshiko , Nagano Hideki , Komono Akira , Sakamoto Ryohei , Morimoto Mitsuaki , Arima Hisatomi , Hasegawa Suguru 
Published: December 2019

AbstractNumbness and pain are currently evaluated using subjective methods such as the visual analogue scale (VAS). PainVision (PV) is an analytical instrument that was designed to quantitatively assess sense perception and nociception in patients. Chemotherapy-induced peripheral neuropathy (CIPN) is one of the most important adverse events that renders prolonged chemotherapy difficult. To assess the features of CIPN, we aimed to compare PV methods with existing methods. A total of 73 patients received oxaliplatin for metastatic colorectal cancer. Registered patients included 37 men and 36 women in the range of 37 to 89 years (median 70). CIPN was evaluated a total of 483 times (median per patient six times). Our study examined the correlation between evaluation methods of CIPN using VAS and PV, respectively. The average VAS (hand), VAS (foot) and PV scores of CIPN were 18.4 (range: 0–100), 23.8 (range: 0–100), and 24.7 (range: 0–496), respectively. VAS (hand), VAS (foot), and FACT/GOG-NTX (NTX2, NTX4 and NTX8) were significantly correlated with PV. PV showed no correlation with a Disk-Criminator or the monofilament test used as a quantitative evaluation. The evaluation of CIPN is complex, and further improvement is required for evaluation with PV.
Complement activation by autoantigen recognition in the growth process of benign prostatic hyperplasia
Authors: Hata Junya , Machida Takeshi , Matsuoka Kanako , Hoshi Seiji , Akaihata Hidenori , Hiraki Hiroyuki , Suzuki Toshiyuki , Ogawa Soichiro , Kataoka Masao , Haga Nobuhiro , Ishibashi Kei , Homma Yoshimi , Sekine Hideharu , Kojima Yoshiyuki 
Published: December 2019

AbstractThe pathophysiology of benign prostatic hyperplasia (BPH) remained unclear. Here, we concentrated on the complement activation in the growth of BPH using a rat model. BPH tissues were harvested from rats after rat urogenital sinus implantation. The local expression and deposition levels of C1q, C3, mannose-binding lectin (MBL), factor B (FB), and C5b-9 in the rat and human BPH tissues were analyzed by real-time RT-PCR, western blotting and immunohistochemistry (IHC). Serum IgG levels in the rat BPH model were analyzed by ELISA, and IHC was used to assess tissue localization. Proteins binding serum IgG autoantibody in the BPH rats were isolated by immunoprecipitation. C1q, C3, MBL, FB and C5b-9 were highly localized in rat BPH tissues compared to normal tissues. In contrast, C3, FB and C5b-9, but not C1q and MBL, were abundantly detected in human BPH tissues compared to normal tissues. Diffuse localization of IgG in rat BPH tissues was found. Heat shock protein 90, annexin, α-smooth muscle actin, and β-actin were identified as targets for IgG autoantibodies in the BPH model. Our results strongly suggested the role for complement activation in the growth process of BPH, likely triggered by classical pathway activation with autoantibodies.
Outcomes of Cardiovascular Surgery Utilizing Heparin versus Direct Thrombin Inhibitors in Cardiopulmonary Bypass in Patients with Previously Diagnosed HIT
Authors: Carlson Daniel S. , Bartholomew John R. , Gomes Marcelo P. , McCrae Keith R. , Chaturvedi Shruti 
Published: December 2019

AbstractHeparin-induced thrombocytopenia (HIT) is a life-threatening complication of heparin therapy. Heparin is generally avoided in patients with a history of HIT; however, it remains the anticoagulant of choice for cardiac surgery requiring cardiopulmonary bypass (CPB) because of limited experience with alternative anticoagulants such as direct thrombin inhibitors (DTIs) during CPB. We report outcomes of surgery requiring CPB (30-day mortality, rate of thrombosis, and hemorrhage) in patients with prior HIT who received either heparin or a DTI intraoperatively. Seventy-two patients with a prior diagnosis of HIT confirmed by a positive serotonin release assay underwent CBP with a positive HIT antibody at the time of surgery. Thirty-day mortality was 0 and 8.5% in the DTI and heparin cohorts (p = 0.277). Thrombotic events occurred in 1 (7.7%) of the patients treated with DTI and 15 (25.4%) receiving heparin (p = 0.164). In the DTI cohort, 7 (53.8%) had minimal bleeding, 5 (38.5%) had mild bleeding, 1 (7.8%) had moderate bleeding, and none had severe bleeding. In the heparin group, 16 (27.1%) had minimal bleeding, 14 (23.7%) had mild bleeding, 25 (42.4%) had moderate bleeding, and 4 (6.8%) had severe bleeding (p = 0.053). DTI was associated with a lower rate of moderate to severe hemorrhage than heparin (odds ratio 0.097 [95% confidence interval 0.011–0.824], p = 0.033) in a logistic regression model adjusted for thrombocytopenia and length on bypass. DTI appears to be safe in selected patients undergoing CPB after a diagnosis of HIT, and was not associated with higher rates of 30-day mortality, thrombosis, or hemorrhage.
Appropriate Timing for Evaluation of the Short-Term Effectiveness of Unicompartmental Knee Arthroplasty
Authors: Inui Hiroshi , Taketomi Shuji , Yamagami Ryota , Kono Kenichi , Kawaguchi Kohei , Nakazato Keiu , Takagi Kentarou , Kage Tomofumi , Tanaka Sakae 
Published: December 2019

AbstractSatisfactory results have been achieved with unicompartmental knee arthroplasty (UKA) for the treatment of isolated unicompartmental knee disease. However, UKA is associated with a significantly higher rate of revision. There is a tendency toward early revision of UKA for persistent pain because surgeons are not aware that outcomes can spontaneously improve with time. The aim of this study was to identify the time period that patients achieved the highest clinical outcomes following UKA. In total, we examined 72 knees that underwent Oxford UKA. We evaluated the range of motion and clinical results including Knee Society Knee Score, Knee Society Function Score (KSFS), and patient-reported scores using the Knee injury and Osteoarthritis Outcome Score (KOOS) at 1, 2, and 3 years postoperatively. The extension angle showed significant recovery from 1 to 2 years postoperatively, but no significant recovery was observed between 2 and 3 years. The flexion angle at 2 years is larger than at 1 year. The KSFS at 3 years is significantly lower than that at 1 year. The KOOS subscale of activities of daily living showed no significant differences among three periods after operation. The subscales of pain, symptom, sports, and quality of life showed significant recovery from 1 to 2 years postoperatively, but no significant recovery was observed from 2 to 3 years. To evaluate the effectiveness of Oxford UKA, surgeons should obtain clinical outcomes 2 years after the operation.
Spectroscopic investigations of negatively charged tin-vacancy centres in diamond
Authors: Görlitz Johannes , Herrmann Dennis , Thiering Gergő , Fuchs Philipp , Gandil Morgane , Iwasaki Takayuki , Taniguchi Takashi , Kieschnick Michael , Meijer Jan , Hatano Mutsuko , Gali Adam , Becher Christoph 
Published: December 2019

Abstract
The recently discovered negatively charged tin-vacancy centre in diamond is a promising candidate for applications in quantum information processing (QIP). We here present a detailed spectroscopic study encompassing single photon emission and polarisation properties, the temperature dependence of emission spectra as well as a detailed analysis of the phonon sideband and Debye–Waller factor. Using photoluminescence excitation spectroscopy we probe an energetically higher lying excited state and prove fully lifetime limited linewidths of single emitters at cryogenic temperatures. For these emitters we also investigate the stability of the charge state under resonant excitation. These results provide a detailed insight into the spectroscopic properties of the SnV− centre and lay the foundation for further studies regarding its suitability in QIP.