bluuebunny/crossref_metadata_2025_split huggingface dataset:
A strategy using SNP linkage analysis for monogenic diseases PGD combined with HLA typing
Authors: Wang Yuqian , Qin Meng , Yan Zhiqiang , Guan Shuo , Kuo Ying , Kong Siming , Nie Yanli , Zhu Xiaohui , Zhi Xu , Qiao Jie , Yan Liying 
Published: May 2020

AbstractPreimplantation genetic diagnosis (PGD) of genetic diseases, combined with human leukocyte antigen (HLA) typing (PGD‐HLA), is a useful technique to have healthy offspring that are compatible with a sibling for hematopoietic stem cells transplantation (HSCT) to treat their genetic diseases. Here, we report a new strategy using single nucleotide polymorphism (SNP) linkage analysis for monogenic disease PGD combined with HLA typing, to simultaneously obtain the information of chromosomal aneuploidy, target mutations and HLA typing through a single low‐depth next generation sequencing (NGS) procedure. In this study, five couples with probands underwent SNP linkage analysis for PGD‐HLA typing were recruited. Within these five couples, two couples fortunately harvested four unaffected and HLA matched embryos with their siblings. After embryo transfer, two healthy neonates were born successfully. Subsequently, cord blood hematopoietic stem cells obtained from these two neonates were collected and frozen for treating their sick siblings. This novel strategy could provide abundant and specific SNPs for each family, therefore linkage information adjacent and even within HLA clusters were apparent. This study offers a highly flexible and precise method which could eliminate misdiagnosis caused by chromosomal recombination of the HLA gene, thus potentially benefit the success rate of HSCT.
Septate hyphae (Rhizoctonia sp) in sputum cytology
Authors: Martínez‐Girón Rafael , Martínez‐Torre Cristina 
Published: May 2020

AbstractThis article discusses the significance and characterisation of long branched yellowish filamentous structures found in a conventional sputum smear stained by the Papanicolaou method from a 55 year old gentleman who started immunosuppressive therapy six months previously when he had a kidney transplant.
Right atrial appendage aneurysm complicated with atrial septal defect: A rare case report
Authors: Duan ShaSha , Li ShuJuan , Sun ZhenTing , Shi YiLu , Fu WenYan , Zhang Lei , Zhao Jie , Wang YaXi , Zhang XiaoShan 
Published: May 2020

AbstractRight atrial appendage aneurysms (RAAAs) are rare heart malformations, presenting as isolated anomalies or co‐existing with other structural heart diseases. We describe a rare case of RAAA complicated with an atrial septal defect (ASD). The diagnosis was established using transthoracic echocardiography and confirmed using cardiac magnetic resonance imaging. To treat the ASD and reduced right atrium volume load, ASD transcatheter closure was performed. On echocardiography performed 3 months post discharge, the RAAA was observed to have reduced in size compared to that presurgery. Six years later, she was in good condition without any adverse events.
Key role of 5‐HT1A receptors in the modulation of the neuronal network underlying the respiratory rhythm generation in lampreys
Authors: Cinelli Elenia , Mutolo Donatella , Iovino Ludovica , Pantaleo Tito , Bongianni Fulvia 
Published: May 2020

AbstractIn mammals, 5‐HTexcitatory respiratory effects imply 5‐HT1A receptor‐mediated disinhibition of pre‐Bötzinger complex neurons. In the lamprey, 5‐HT1A receptors are involved in the neural control of locomotion, but their role in the respiratory regulation, particularly at the level of the putative respiratory rhythm generator, the paratrigeminal respiratory group (pTRG), is not known. We here investigate the respiratory function of inhibitory 5‐HT1A receptors within the pTRG of the isolated brainstem of the adult lamprey. The 5‐HT1A receptor agonists either bath applied or microinjected into the pTRG did not cause significant effects. However, the selective 5‐HT1A receptor antagonist (S)‐WAY 100135 bath applied or microinjected into the pTRG induced depressing respiratory effects or even apnoea, thus revealing that 5‐HT exerts a 5‐HT1A receptor‐mediated potent tonic influence on respiration and contributes to maintain baseline levels of respiratory activity. Microinjections of strychnine or bicuculline, either alone or in combination, into the pTRG prevented (S)‐WAY 100135‐induced apnoea. In addition, immunohistochemical studies corroborate the present findings suggesting that 5‐HT1A receptors are widely expressed in close apposition to the soma of glycine‐immunoreactive cells located within the pTRG region. The results show that in the lamprey respiratory network, 5‐HT exerts a tonic influence on respiration by a potent inhibitory control on both GABAergic and glycinergic mechanisms. The observed disinhibitory effects resemble the excitatory respiratory modulation exerted by 5‐HT1A receptor‐mediated inhibition of glycinergic and/or GABAergic neurons present in mammals, supporting the notion that some features of the neuronal network subserving respiratory rhythm generation are highly conserved throughout phylogeny.
Filtration efficiency of surgical and FFP3 masks against composite dust
Authors: Breul Sebastian , Van Landuyt Kirsten L. , Reichl Franz X. , Högg Christof , Hoet Peter , Godderis Lode , Van Meerbeek Bart , Cokic Stevan M. 
Published: May 2020

The aim of this study was to investigate the protection efficiency of two types of face masks against composite dust and to characterize the particles that penetrated through the masks. Composite dust was created by grinding a commercial nano‐filled composite in a plexiglass box without using water cooling or high vacuum evacuation, in order to obtain a worst‐case exposure. Dust particles were collected using a personal inhalable aerosol sampler (IOM) fixed inside a custom‐made phantom head. Surgical and filtering facepiece (FFP3) masks were tested, and the situation without a mask served as control. The IOM sampler contained a cassette with two filters to collect large inhalable (4–100 µm) and respirable dust particles (<4 µm). The amount of particles was determined gravimetrically by weighing filters before and after composite grinding, and further characterized by electron microscopy. Particle collection for both inhalable and respirable dust was the highest when no mask was used, and the lowest with the use of a FFP3 mask. Different sizes and shapes of particles were observed, with the largest particles (>1 µm) being seen when no mask was applied, whereas only nanoparticles could be detected when either type of face mask was applied. Even though FFP3 masks showed a higher filtration efficacy than surgical masks of the inhalable dust fraction, penetration of a small respirable particle fraction was inevitable for both masks.
NPM resistance: A political intervention
Authors: Nyland Kari , Ahlgren Per Christian , Lapsley Irvine 
Published: May 2020

AbstractThis paper offers a unique perspective on NPM enactment. NPM is the key means by which neoliberal policies penetrate society and there is now a considerable literature on NPM in many economies. The influence of NPM tends to be depicted as significant, embedded, and irreversible. This study offers findings of resistance and rejection of NPM by a cadre of politicians who did not accept the trappings of NPM. The importance of accounting and metrics was rejected by politicians in this study. These actions also influenced the actions of professionals in health care organizations.
Can we project changes in fish abundance and distribution in response to climate?
Authors: Fernandes Jose A. , Rutterford Louise , Simpson Stephen D. , Butenschön Momme , Frölicher Thomas L. , Yool Andrew , Cheung William W. L. , Grant Alastair 
Published: May 2020

AbstractLarge‐scale and long‐term changes in fish abundance and distribution in response to climate change have been simulated using both statistical and process‐based models. However, national and regional fisheries management requires also shorter term projections on smaller spatial scales, and these need to be validated against fisheries data. A 26‐year time series of fish surveys with high spatial resolution in the North‐East Atlantic provides a unique opportunity to assess the ability of models to correctly simulate the changes in fish distribution and abundance that occurred in response to climate variability and change. We use a dynamic bioclimate envelope model forced by physical–biogeochemical output from eight ocean models to simulate changes in fish abundance and distribution at scales down to a spatial resolution of 0.5°. When comparing with these simulations with annual fish survey data, we found the largest differences at the 0.5° scale. Differences between fishery model runs driven by different biogeochemical models decrease dramatically when results are aggregated to larger scales (e.g. the whole North Sea), to total catches rather than individual species or when the ensemble mean instead of individual simulations are used. Recent improvements in the fidelity of biogeochemical models translate into lower error rates in the fisheries simulations. However, predictions based on different biogeochemical models are often more similar to each other than they are to the survey data, except for some pelagic species. We conclude that model results can be used to guide fisheries management at larger spatial scales, but more caution is needed at smaller scales.
Pyoderma gangrenosum: From historical perspectives to emerging investigations
Authors: Hobbs Misty M. , Ortega‐Loayza Alex G. 
Published: May 2020

AbstractPyoderma gangrenosum (PG) is a rare disease of unknown aetiology, first described over a century ago. Initially thought to have an infectious cause, and now primarily considered an autoinflammatory condition, PG continues to be poorly understood, commonly misdiagnosed, and difficult to treat. In this review, we discuss the journey of our understanding of PG to date, including first descriptions, challenges with diagnosis, presumed pathogenesis, and treatments used. We highlight major historical landmarks and their importance, explain the rationale behind current investigations, note outstanding gaps in knowledge, and explore the future directions of PG research. We summarise what we have known, what we are working on knowing, and what we have yet to explore about PG, illustrating overall trends to invigorate future research.
Hydrolytic resistance of K2O–PbO–SiO2 glasses in aqueous and high‐humidity environments
Authors: Palomar Teresa , Mosa Jadra , Aparicio Mario 
Published: May 2020

AbstractSome historical glasses (lead‐wood ash glasses, lead‐crystal glasses…) are silicate glasses with high content of lead and potassium. This work presents the evaluation of the chemical stability of high‐lead glasses in a high relative humidity atmosphere and as result of aqueous immersion. In both situations, the alteration mechanism begins with the lixiviation of alkali metal and lead ions, followed by the hydrolytic attack of the silica glass network. According to the results, the glasses with a higher content of lead show the fastest degradation due to their higher hygroscopicity. Environmental CO2 can be dissolved in the adsorbed water and favor the formation of intermediate degradation compounds.
CircRNA WHSC1 targets the miR‐646/NPM1 pathway to promote the development of endometrial cancer
Authors: Liu Yao , Chen Shuo , Zong Zhi‐Hong , Guan Xue , Zhao Yang 
Published: May 2020

AbstractCircular RNAs (circRNAs) play important roles in human cancer progression. Their high stability and tissue specificity make circRNAs important molecular targets for clinical diagnosis, treatment and prognosis. However, the functions and molecular mechanisms of circRNA WHSC1 in endometrial cancer are unknown. CircWHSC1 expression in normal endometrial and endometrial cancer tissues was detected using PCR. Overexpression or knockdown of circWHSC1 in endometrial cancer cell lines HEC‐1B or Ishikawa, respectively, cell function experiments were used to detect the impact of circWHSC1 on endometrial cancer cells. A nude mouse xenograft model was used to detect changes in tumorigenesis of HEC‐1B cells after circWHSC1 overexpression. Bioinformatics and dual luciferase reporter gene technology were used to predict and validate the sponging ability of circWHSC1 on microRNAs. Gene expression changes were detected by using Western blotting. CircWHSC1 expression was increased in endometrial cancer tissues. CircWHSC1 overexpression promoted the proliferation, migration and invasion of endometrial cancer cells and decreased apoptosis. CircWHSC1 knockdown had the opposite effect. CircWHSC1 overexpressed nude mice showed increased tumorigenicity. Bioinformatics predicted that circWHSC1 binds to miR‐646, which was confirmed using luciferase reporter gene assays. High expression of miR‐646 could reverse the effect of circWHSC1 on endometrial cancer cells. Western blotting showed increased or decreased levels of nucleophosmin 1 (NPM1), an miR‐646 downstream target, after circWHSC1 overexpression or knockdown, respectively. CircWHSC1 promotes endometrial cancer development through sponging miR‐646 and targeting NPM1.
Adaptable fiberglass post after 3D guided endodontic treatment: Novel approaches in restorative dentistry
Authors: Silva Amanda Stephanie , Carvalho Santos Alice Cecília , de Sousa Caneschi Camila , Machado Vinícius Carvalho , Moreira Allyson Nogueira , dos Santos Alves Morgan Luís Fernando , Tavares Warley Luciano Fonseca 
Published: May 2020

AbstractObjectiveThe rehabilitation of extensively damaged teeth with great structural loss and calcified root canals represents a challenge for dentistry. Classically, this restorative procedure of endodontically treated teeth involves intraradicular post placement. Retentive function post that can closely adapt to the root canal without the excessive wear of intraradicular dentin has a fundamental importance to ensure the longevity of the restoration.Clinical considerationsThis report presents a clinical case of 3D guided access to a severely calcified pulp canal followed by the cementation of a fiberglass post that is adaptable to the root canal and prosthetic rehabilitation with full ceramic crown.Conclusions3D guided access allowed conservative and safe root canal treatment. The adaptable fiberglass post meets the specific needs of the described case and has a simple technique and low cost.Clinical significanceGuided endodontics is a minimally invasive approach for teeth with calcified root canals. The post used in this study meets the demands of teeth endodontically treated with conservative techniques. The professional should not need to adapt the anatomy of the root canal to the anatomical reality of the post. This one adapts itself to the shape of the canal.
Nursing Home Responses to Performance‐based Accountability: Results of a National Survey
Authors: Shetty Kanaka D. , Tolpadi Anagha A. , Robbins Michael W. , Taylor Erin A. , Campbell Kyle N. , Damberg Cheryl L. 
Published: May 2020

OBJECTIVESThe Centers for Medicare & Medicaid Services (CMS) Nursing Home Quality Initiative aims to improve quality through performance measurement. We describe quality improvement (QI) changes that skilled nursing facilities (SNFs) reported making in response to CMS performance measurements and whether reported QI changes were associated with better performance on CMS performance measures.DESIGNNationally representative survey.SETTINGA total of 15,475 SNFs that reported quality performance on Nursing Home Compare in 2016.PARTICIPANTSA total of 1,182 SNFs (58% of random sample of 2,045 SNFs).MEASUREMENTSAdoption of 22 possible QI changes, grouped into seven categories (organizational culture, health information technology, care process redesign, provider incentives, changes to staffing responsibilities, performance monitoring, and measure‐specific QI initiatives and technical assistance); performance on the CMS Nursing Home Compare Five‐Star Quality Rating System's quality measure rating.RESULTSSNFs reported making an average of 13 QI changes (interquartile range = 11‐16 changes). SNFs mostly commonly reported becoming a learning organization (87%) and providing training to staff on QI strategies (87%). After controlling for patient and facility characteristics, larger SNFs were more likely to obtain assistance on measure reporting from QI organizations and use provider champions than smaller SNFs by 14 and 11 percentage points, respectively. Rural SNFs and SNFs with higher proportions of disabled, black, or Hispanic residents adopted QI changes at similar rates as other SNFs. Of the 22 QI changes, 20 were considered at least somewhat helpful by more than 80% of adopting SNFs. Implementation of all 22 QI changes (vs no changes) was associated with a .48‐star higher quality measure rating (95% confidence interval = .003‐.98 stars; P = .05).CONCLUSIONIn response to CMS measurement programs, SNFs reported making substantial QI investments that were associated with better performance on CMS quality measures. To guide future SNF investments in QI, work is needed to identify the QI changes that yield the greatest performance improvements.
Management of polyethylene glycol solution aspiration using bronchoscopic lavage in a dog
Authors: Aguayo Santaella Daniela M. , Olin Shelly J. , Willard Michael D. , Whittemore Jacqueline C. 
Published: May 2020

AbstractPolyethylene glycol lavage solutions are used for colonic preparation in dogs and are considered relatively safe. Aspiration is an uncommon but potentially devastating complication of polyethylene glycol administration. Full recovery is possible and often rapid in people treated with bronchoalveolar lavage. A healthy 2‐year‐old male Beagle used in an endoscopy teaching laboratory aspirated a small amount of polyethylene glycol lavage solution. Although initially appearing unaffected, the dog quickly became hypoxemic. Bronchoscopy was used to lavage the lungs and aspirate tracheal/pulmonary fluid 5 times over the course of 45 minutes. The dog completely recovered. This report presents the successful treatment of polyethylene glycol aspiration in a dog. Although the seriousness of aspiration might not be immediately evident, bronchoscopy and lavage should be pursued because of the rapidly progressive nature of polyethylene glycol‐induced pulmonary edema.
Convective activity in a Martian magma chamber recorded by P‐zoning in Tissint olivine
Authors: Mari N. , Hallis L. J. , Daly L. , Lee M. R. 
Published: May 2020

AbstractThe Tissint Martian meteorite is an unusual depleted olivine‐phyric shergottite, reportedly sourced from a mantle‐derived melt within a deep magma chamber. Here, we report major and trace element data for Tissint olivine and pyroxene, and use these data to provide new insights into the dynamics of the Tissint magma chamber. The presence of irregularly spaced oscillatory phosphorous (P)‐rich bands in olivine, along with geochemical evidence indicative of a closed magmatic system, implies that the olivine grains were subject to solute trapping caused by vigorous crystal convection within the Tissint magma chamber. Calculated equilibration temperatures for the earliest crystallizing (antecrystic) olivine cores suggest a Tissint magma source temperature of 1680 °C, and a local Martian mantle temperature of 1560 °C during the late Amazonian—the latter being consistent with the ambient mantle temperature of Archean Earth.
Sutural loading in bone‐ versus dental‐borne rapid palatal expansion: An ex vivo study
Authors: Fox Gavin C. , Jones Thomas A. , Wilson John M. , Claro Wanda I. , Williams Richard A. , Trojan Terry M. , Al Dayeh Ayman 
Published: May 2020

AbstractObjectivesTo measure and compare the success rate and strains generated during bone‐ (BRPE) and dental‐borne rapid palatal expansion (DRPE) at the alveolar bone, zygomaticomaxillary (ZMS) and internasal (INS) sutures. Additionally, the magnitude and the pattern of midpalatal suture (MPS) separation in the 2 groups was assessed.Setting and Sample PopulationThe study was performed ex vivo using 28 pig heads.Materials and MethodsHeads were dissected, and the MPS, ZMS, INS and the alveolar bone were exposed. A differential‐variable‐reluctance‐transducer (DVRT) was installed across the MPS, and single‐element strain gauges were installed at the remaining sites. Expanders were placed and activated at one turn per minute for 30 turns. Strains at the alveolar bone and the sutures and the separation of the MPS were measured.ResultsSuccessful expansion of the MPS was achieved in 69% of the BRPE subjects compared to 27% in the DRPE group. The average separation of the MPS was higher (230 ± 109 µm per turn vs. 79 ± 61 µm) and the MPS opening happened at an earlier stage of expansion in the BRPE. Higher strains at the ZMS were seen in the BRPE group, while higher strain at the alveolar bone was found in the DRPE group.ConclusionsThe BRPE group demonstrated more successful and effective expansion of the MPS. Higher strain was found at the alveolar bone in the DRPE. A tendency for higher strain at the ZMS was noticed in the BRPE.
Predictors of the Analgesic Efficacy of CT‐Guided Percutaneous Pulsed Radiofrequency Treatment of Gasserian Ganglion in Patients With Idiopathic Trigeminal Neuralgia
Authors: Ren Hao , Zhao Chunmei , Jia Zipu , Bian Jiajia , Luo Fang 
Published: May 2020

AbstractAimsAlthough pulsed radiofrequency (PRF) has few postoperative adverse reactions, its analgesic efficacy for trigeminal neuralgia (TN) is not always guaranteed. The response rate of PRF targeting the Gasserian ganglion for patients with TN varies. This study aims to identify the predictors of the analgesic efficacy of CT‐guided percutaneous PRF in patients with idiopathic TN.MethodsThe patients with idiopathic TN who failed to respond to conservative treatment and underwent CT‐guided percutaneous PRF treatment of the Gasserian ganglion from July 2008 to August 2018 at our pain clinic were screened. A positive response was defined as a ≥50% reduction in the pain numeric rating scale (NRS) score from baseline. The demographics and other pretreatment clinical data were analyzed by logistic regression analysis to identify the predictors of a positive response.ResultsOf the total 102 patients with idiopathic TN who were evaluated in this study, 57 patients (55.9%) were positively responsive to our treatment after 1 year. The binary logistic regression analysis revealed that the positive response to a prior peripheral branch nerve block of the trigeminal nerve with steroid and local anesthetic agents was an independent predictor of the analgesic efficacy of PRF treatment (odds ratio [OR] = 3.685, 95% confidence interval [CI] = 1.583 to 8.577, P = 0.002). The disease duration of TN was also included in the multivariate regression model, although the P value was 0.058 (OR 0.807, 95% CI 0.646 to 1.007).ConclusionFor patients who previously had positive responses to peripheral branch nerve block of the trigeminal nerve, PRF is likely to have better efficacy.