bluuebunny/crossref_metadata_2025_split huggingface dataset:
Anti-Cancer Activity of Piperine Against Colon Carcinogenesis Via Modulation of NF-κB/Nrf-2/Keap1/HO-1 Signalling Pathways
Authors: Rehman Muneeb U , Rashid Summya , Arafah Azher , Qamar Wajhul , Al-Saffar Rana M , Ahmad Ajaz , Almatroudi Nada M , Alqahtani Saeed M Abdullah , Rashid Shahzada Mudasir , Ahmad Sheikh Bilal 
Published: June 2020

Abstract
Background: Colon cancer is the most common cancer in men and women globally, killing millions of people annually. Though widespread development has been made in the management of colorectal cancer, still there is an urgency to find novel targets for effective treatment of these patients. Piperine is an alkaloid found in black pepper having anticancer, anti-oxidant and anti-inflammatory activities. Nuclear factor-erythroid 2–kelch-like ECH-associated protein 1(Nrf-2/Keap1)/ Heme-oxygenase1 (HO-1) signaling pathway plays a vital part in shielding cells from the injury of intracellular oxidative stress and inflammation. A potential cross-talk between Nrf-2 and NF-κB pathways is recognized during cancerous growth and expansion. We studied this pathway extensively in the present study to discover novel targets in prevention of chemically induced colon cancer with piperine.Results: We found that NF-κB inhibition occurs by the activation of Nrf-2 blocks downstream inflammatory mediators/cytokines (TNF-α, IL-6, IL-1β, Cox-2, PGE2, iNOS, NO, MPO) and triggers antioxidant response machinery (HO-1, NQO-1 GSH, GR, GPx, CAT, SOD), scavenges ROS, decreases lipid peroxidation. Histological findings further validated our defensive effects of piperine on colon carcinogenesis. Piperine down-regulates CEA, MDF and ACF, markers of precancerous lesions in colon.Conclusion: Our results indicate that piperine may be an effective molecule for the prophylactic treatment of colon carcinogenesis by targeting NF-κB/Nrf-2/Keap1/HO-1 pathway as a progressive strategy in preclusion and effective treatment of colorectal cancer.
COUP-TFI Deletion Affects Angiogenesis and Apoptosis Related Genes Expression in Ex-Vivo Mice Placenta
Authors: Viola Luigi , Marzinotto Stefania , Bertacchi Michele , Londero Ambrogio P , Orsaria Maria , Bertozzi Serena , Driul Lorenza , Loreto Carla Di , Studer Michele , Mariuzzi Laura , Fruscalzo Arrigo 
Published: June 2020

Abstract
Background: Chicken Ovalbumin Upstream Promoter-Transcription Factor I (COUP-TFI) is a member of the steroid/thyroid nuclear receptor superfamily. Aim of the study was to investigate whether the absence of this gene affects placental development and fetal growth in a mouse model. For this scope placentas of COUP-TFI-knockout (COUP-TFI –/–) and wild-type (WT) were collected at the 18.5 days post-coitum. mRNA amount of following genes known to be involved in different key molecular pathways were evaluated: Bax and Bcl-2 (apoptosis), p21 and p53 (proliferation), VEGF-A, PlGF, HIF1α, Flt-1, ENG, and INHA (angiogenesis). Mice litter weight at birth was also compared.Results: RT-qPCR analysis showed an increased mRNA expression of VEGF-A and Bax in placental tissue of COUP-TFI –/– mice compared to WT mice. Also, a lost in the positive correlation between Bcl-2 and INHA, p21 and ENG, as well as HIF1α and Flt-1 mRNA expression in COUP-TFI null mutants was found. Finally, the mean weight of WT mice was 1.6 g (±0.14) compared to 1.3 grams (±0.13) of COUP-TFI –/– mice (p<0.05). Conclusions: Our results show that COUP-TFI deletion is associated to a lower birth weight in mouse and an increased placental mRNA expression of pro-apoptotic Bax and pro-angiogenetic VEGF-A genes.
Prevalence of Anxiety and Depression in General Population of Iran during the COVID-19 Pandemic: A Web-Based Cross-Sectional Study
Authors: Maroufizadeh Saman , Pourshaikhian Majid , Pourramzani Ali , Sheikholeslami Farzaneh , Moghadamnia Mohammad Taghi , Alavi Seyed Amirhossein 
Published: June 2020

Abstract
Background: Coronavirus disease 2019 (COVID-19) is a global public health emergency and may result in psychological problems such as anxiety and depression in general population. Therefore, this study aimed to determine the prevalence of anxiety and depression and their associated factors in general population of Iran during the COVID-19 pandemic.Methods: We conducted this web-based cross-sectional study on 5328 individuals in Iran, between 17 and 29 April 2020. Data were collected through an anonymous online questionnaire via social media like WhatsApp and Telegram. The online survey collected information on demographic characteristics, COVID-19-related variables, and anxiety and depression. Anxiety and depression were assessed using the Generalized Anxiety Disorder-7 (GAD-7) and the Patient Health Questionnaire-9 (PHQ-9), respectively. Simple and multiple logistic regression analyses were performed to determine independent predictors of anxiety and depression.Results: The mean GAD-7 and PHQ-9 total scores were 7.17 (SD=5.42) and 7.80 (SD=6.68), respectively. The prevalence of anxiety, depression, and comorbid anxiety-depression were 30.1%, 33.4%, and 22.1%, respectively. According to the adjusted analysis, anxiety was significantly associated with being female, being young and middle-aged, being unemployed or housewife, having chronic diseases, long time thinking about COVID-19, having family members, friends, and/or relatives infected with COVI-19, and death of family members, relatives or friends due to COVID-19. Same results were also found for depression. Furthermore, depression was associated with being single, being resident in urban area, and having high risk individual in family. Conclusion: The prevalence of anxiety and depression were considerably high in general population of Iran during the COVID-19 pandemic. In addition, the findings suggest that more attention needs to be paid to vulnerable groups such as women, young/middle-aged adults, unemployed people, people with chronic disease. Thus, a holistic approach, including management policies, psychosocial interventions, training, support, and tele-mental health, is absolutely crucial to reduce mental health problems of the COVID-19 and any outbreaks of other infectious diseases.
Pengembangan Blog Pembelajaran Kimia Berbasis Contextual Teaching Learning (CTL) Materi Reaksi Oksidasi-Reduksi
Authors: Sulistyani Yeni , Nirwana Ratih Rizqi 
Published: June 2020

Pelaksanaan pembelajaran harus mampu menyesuaikan diri agar tujuan pendidikan dapat <em>tercapai. Kegiatan pembelajaran peserta didik tidak hanya dapat dilakukan di sekolah dan pada jam pelajaran saja. Kemudahan akses internet seharusnya dapat dimanfaatkan peserta didik sebagai salah satu sumber belajar. Saat ini sudah tersedia banyak situs yang menampilkan materi-materi pelajaran, namun masih banyak informasi yang tidak sesuai. Sehingga diperlukan pengembangan secara optimal agar bahan ajar yang diakses siswa tepat. Penggunaan blog sebagai media diharapkan dapat memudahkan peserta didik untuk mengakses materi pelajaran. Materi konsep reaksi oksidasi reduksi merupakan salah satu materi yang dianggap sulit peserta didik. Pengembangan blog pembelajaran ini diharapkan mampu meningkatkan pemahaman peserta didik mengenai konsep reaksi oksidasi reduksi. Penelitian ini menggunakan metode pengembangan Research and Development (R n D), dengan model Reisser dan Molenda (1990-an) yaitu model ADDIE yang meliputi analysis, design, development, implementation, dan evaluation. Blog pembelajaran yang telah dikembangkan mempunyai kategori sangat tinggi atau sangat efektif berdasarkan hasil belajar peserta didik yang meliputi kognitif sebesar 80,04%, afektif sebesar 85,8 %, psikomotorik sebesar 84%, dan tanggapan peserta didik sebesar 85,83 %. Blog pembelajaran ini layak digunakan sebagai media pembelajaran penunjang</em><em> pembelajaran kimia berbasis contextual teaching and learning pada materi konsep reaksi oksidasi-reduksi</em>
Keterampilan Berpikir Tingkat Tinggi Peserta Didik Kelas XI dengan Model (Experiential Learning) pada Materi Titrasi Asam Basa
Authors: Khoirussaadah Khoir Saadah , Hakim Fachri 
Published: June 2020

Tren dalam studi matematika dan sains internasional menunjukkan 95% peserta didik Indonesia berada pada kemampuan berpikir tingkat tinggi yang rendah. Upaya untuk menyikapi hal ini, perlu dilakukan inovasi untuk meningkatkan berpikir tingkat tinggi peserta didik. Model pembelajaran (<em>Experiential Learning</em>) merupakan salah satu model pembelajaran yang menciptakan suasana pembelajaran yang kondusif, dan pembelajaran yang dikaitkan dengan pengalaman lansung mampu meningkatkan penguasaan konsep peserta didik dan akan berpengaruh terhadap berpikir tingkat tinggi peserta didik. Jenis penelitian eksperimen, sampel penelitian yaitu peserta didik kelas XI IPA 1 kelas kontrol dan kelas XI IPA 2 kelas eksperimen. Instrumen pengumpulan data berupa soal pretest, posttest dan wawancara. Hasil penelitian menunjukkan terdapat perbedaan berpikir tingkat tinggi peserta didik yang menggunakan model pembelajaran berbasis pengalaman (<em>Experiential Learning</em>) dengan peserta didik yang menggunakan metode konvensional. Hal tersebut sesuai dengan hasil pretest peserta didik mampu menjawab 25% soal jenjang C4, 51% soal jenjang C5, dan 52% soal jenjnag C6, setelah menggunakan model pembelajaran berbasis pengalaman (<em>Experiential Learning</em>) mengalami kenaikan dimana peserta didik kelas eksperimen mampu menjawab 44% soal jenjang C4, 74% soal jenjang C5, dan 72% jenjang soal C6.
Pengembangan Modul Kimia Berbasis Teaching Factory pada materi Redoks Terintegrasi Kompetensi Keahlian Teknik Fabrikasi Logam dan Manufaktur
Authors: Fahmi Khoerussani Nur 
Published: June 2020

<span lang="IN">Penelitian ini didasarkan karena kurangnya sumber belajar kimia yang dihubungkan dengan materi kejuruan SMK serta didasarkan pada pembelajaran teaching factory yang diterapkan di sekolah belum berjalan maksimal karena tidak ada produk yang dihasilkan dalam pembelajaran kimia. Tujuan penelitian ini ialah menghasilkan modul pembelajaran kimia berbasis teaching factory pada Materi Redoks Terintegrasi Kompetensi Keahlian Teknik Fabrikasi Logam dan Manufaktur serta untuk mengetahui kualitas modul yang dikembangkan. Penelitian pengembangan ini menggunakan jenis penelitian RnD (Research and Development) dengan model pengembangan 4D yang dikembangkan oleh Thiagarajan meliputi tahapan define, design, develop, dan disseminate. Tetapi pada penelitian ini hanya sampai pada tahap develop bagian initial testing. Subjek dari penelitian ini adalah peserta didik kelas X SMK Negeri 7 Semarang kompetensi keahlian TFM. Hasil uji kualitas modul menunjukkan bahwa modul dikategorikan sangat layak oleh validator materi dan media dengan nilai koefisien Aiken’s V sebesar 0,89. Hasil respon angket tanggapan peserta didik dikategorikan baik dengan nilai skor rata-rata 83,11. Berdasarkan data tersebut, modul kimia berbasis teaching factory terintegrasi kompetensi kahlian TFM dinyatakan layak untuk digunakan.</span>
Performance evaluation of potential inland flood management options through a three-way linked hydrodynamic modelling framework for a coastal urban watershed
Authors: Ghosh Mousumi , Mohanty Mohit Prakash , Kishore Pushpendra , Karmakar Subhankar 
Published: June 2020

AbstractThis study proposes a novel comprehensive hydrodynamic flood modelling framework over Mithi river watershed in Mumbai, India, a coastal urban area, to reduce the inundation extent by incorporation of different inland hydraulic scenarios. First, the study addresses the issue of data scarcity by adapting alternate robust techniques to estimate design rainfall, tidal elevation and discharge, the key inputs for a flood model. Following that, a three-way linked flood model has been developed in the MIKE FLOOD platform, considering river, stormwater, overland flow and tidal influence to generate flood inundation and subsequently hazard maps for various inland hydraulic scenarios, by incorporating different feasible cross-sections and lining materials. The flood inundation and hazard maps have been derived for 10-, 50- and 200-year return periods of design rainfall, discharge and tide to identify the best possible flood-reducing hydraulic scenario. It is observed that a ‘trapezoidal river cross-section lined with concrete’ relatively maximizes the reduction in flooding extent. The proposed framework can be implemented as an effective flood mitigation strategy in data-scarce, densely populated and space-constrained areas.
Removal of COD, NH4-N, and perfluorinated compounds from wastewater treatment plant effluent using ZnO-coated activated carbon
Authors: Tang Jiawei , Liu Yu , Su Peidong , Quan Jingwei , Hu Yufeng , Wang Wenqian , Zhang Chunhui 
Published: June 2020

Abstract
This study investigated the removal of chemical oxygen demand (COD), NH4-N, and perfluorinated compounds (PFCs) in the effluent from a wastewater treatment plant (WWTP) using ZnO coated activated carbon (ZnO/AC). Results suggested that the optimal dosage of the ZnO/AC was 0.8 g/L within 240 min of contact time, at which the maximum removal efficiency of COD was approximately 86.8%, while the removal efficiencies of PFOA and PFOS reached 86.5% and 82.1%. In comparison, the removal efficiencies of NH4-N, PFBA, and PFBS were lower, at approximately 47.9%, 44.0%, and 55.4%, respectively. In addition, COD was preferentially adsorbed before PFCs and NH4-N, when the contact time ranged from 0 to 180 min, and the order of PFCs removal showed a positive correlation with C-F chain length. The kinetic study revealed that the removal of COD, NH4-N, and PFCs could be better depicted and predicted by the Lagergren quasi-second order dynamic model with high correlation coefficients, which involved liquid membrane diffusion, intraparticle diffusion, and photocatalytic reactions. The saturated ZnO/AC was finally regenerated using ultrasound for 3 h and retained excellent performance, which proved it could be considered as an effective and alternative technology.
Exercise and Supplementation of Black Mulberry Fruit Extract, Sunflower Seed and Pumpkin Seed Enhance Cognitive Performance among Sedentary University Students
Authors: Shalan Nor A.A.M. , Rahim Norhazira A. , Mohamad Nur I. 
Published: June 2020

Background:
sedentary lifestyle has been found to influence cognitive impairment,
whereas physical activity and diet have been found to reduce the negative impacts of a sedentary lifestyle.

Objectives:
This study aims to examine the synergistic effects of the black mulberry, sunflower seed,
and pumpkin seed supplements, including exercise on cognitive performance among sedentary university
students.

Subjects and Methods:
Participants (n=36) were randomly assigned to receive black mulberry, sunflower seed,
and pumpkin seed with and without exercise for 60 days. Then, they were required to complete cognitive
task assessment for domain attention (visual reaction time and auditory reaction time), perception
(fast counting and basic music) and executive (Eriksen flanker task and Stroop test) on day 0
(baseline), 30, and 60 of the experiment. Following that, blood samples were collected and analyzed
for malondialdehyde serum concentration as an oxidative stress marker.

Results:
All participants showed significant faster reaction time in cognitive tasks for domain attention
and basic music test for domain perception on day 30 and 60 compared to day 0. However, no
significant changes were observed within group, on cognitive task for domain executive. In comparison
between the groups, participants in group treatment showed significant faster reaction time for
cognitive tasks for domain attention and for Erikson flanker task for domain executive as compared
to group exercise alone, and group treatment with exercise. For domain perception (basic music test),
participants in group treatment with exercise achieved faster reaction time compared to group treatment
and exercise alone. Despite the reduction of malondialdehyde concentration in all groups, no
significant difference was found between them.

Conclusion:
It was indicated from the findings that the consumption of black mulberry fruit extract,
sunflower seeds, and pumpkin seeds with and without exercise independently enhanced attention,
perception, and executive function among sedentary young adults.
Synthesis of New Zerumbone Hydrazones and Their In-vitro Anticancer Activity
Authors: Vu Vu V. , Vu Tran K. 
Published: June 2020

Background:
A series of new zerumbone hydrazones 5a-f and 9a-f have been synthesized
in via an in situ procedure in high yields. The structure of synthesized compounds has been
confirmed using 1H, 13C NMR and HR-MS. The bioassay result showed that several compounds exhibited
cytotoxic effects against three human cancer cell lines, including HepG-2, SK-LU-1, and
MCF-7. Compound 9a showed the best cytotoxic effect against HepG-2, SK-LU-1, and MCF-7
with IC50 values of 8.20, 6.66, and 9.35 μM, respectively.

Objective:
This study aims at developing new zerumbone hydrazones as anticancer agents based
on zerumbone, a natural compound wildly growing in Vietnam.

Methods:
A series of new zerumbone hydrazones was designed, synthesized, and evaluated for cytotoxicity
against three human cancer cell lines, including HepG-2, MCF-7, and SKLu-1, using the
MTT method.

Results:
The bioassay result showed that several compounds exhibited cytotoxic effects against
three human cancer cell lines, including HepG-2, SK-LU-1, and MCF-7. Especially, compound 9a
displayed the best cytotoxic effect against HepG-2, SK-LU-1, and MCF-7 with IC50 values of 8.20,
6.66, and 9.35 μM, respectively.

Conclusion:
The research results suggest that some compounds could be considered as leads for
the future design of zerumbone hydrazones in which bio-isosteric replacements in theortho position
of the phenyl ring could be performed to improve the cytotoxic activity.
An Overview on Resistivity, Diagnostic Challenges and Zoonotic Significance of: Mycobacterium avium ssp. paratuberculosis (MAP)
Authors: Adhikari Niran 
Published: June 2020

Background:
Mycobacterium avium ssp. paratuberculosis (MAP) is a gram-positive, contagious, rod-shaped intracellular pathogen.

Methods:
MAP is the etiologic agent of Johne’s disease in cattle, and has tremendous economic effect in the ruminant industry. Simultaneously, the MAP has also been suspected as a cause of Crohn’s disease in humans.

Results:
There has been a challenge in the diagnosis of MAP due to its long incubation period, unknown pathogenesis & cross-reactivity among its closely related sub-species. The survival of MAP inside the host macrophages & monocytes, is still unclear. Resistivity & survival of MAP in the outside environment is also high.

Conclusion:
Thus, more research about its pathogenesis, control, and potential role as a zoonotic pathogen must be carried out in future.
Rhanterium suaveolens, Vitamin E and C Pretreatment Prevents Valproic Acid Induced Renal Oxidant Damage
Authors: Amrani Amel , Benaissa Ouahiba , Boubekri Nassima , Benayache Fadila , Benayache Samir , Zama Djamila 
Published: June 2020

Background:
Long-term administration of valproic acid (VPA) is known to promote renal
tubular injury mediated by an increase in renal oxidative stress. Recent evidence indicates that natural
antioxidants are alternative to attenuate oxidative stress and kidney damage.

Objective:
This study was performed to investigate the protective effects of n-butanol extract of
Rhanterium suaveolens, vitamin E (Vit E), and vitamin C (Vit C) against VPA induced nephrotoxicity
in mice.

Methods:
Mice were randomly divided into 6 groups (n: 8) and treated daily for 12 days. They received
VPA (300 mg/kg intraperitoneally (ip)), buthanolic extract (100 mg/kg), Vit E (100 mg/kg),
and Vit C (16.66 mg/kg) 1h prior to administration of VPA. On day 13, blood and Kidneys samples
were analyzed for biomarker levels and histopathological changes. Kidneys homogenates were used
for the determination of oxidative stress parameters that include malondialdehyde (MDA),
glutathione (GSH) level and Glutathione Peroxidase (GPx) activity.

Results:
Treatment with VPA showed a significant increase in the levels of serum creatinine, urea
and malondialdehyde (MDA) and decreasing the enzymatic activity (GPx) as well as GSH levels in
the kidney with marked necrotic epithelial cells and infiltration in kidney sections as compared to the
control group. Pretreatment with the n-butanol extract of R. suaveolens, Vit C or Vit E 1 h prior to
administration of VPA showed a significant decrease in the levels of serum creatinine, urea, and
MDA, as well as an improvement in the antioxidant elements and histological changes compared to
those previously seen in the group treated with VPA alone.

Conclusion:
It is concluded that n-butanol extract of R. suaveolens, Vit C and Vit E pretreatment effectively
improved renal function and tissue oxidative damage caused by VPA.
A Glance on Rare Earth Oxides: Importance, Reserves, De mand,
Applications, Critical Uncertainties, Global Economy, and Zeta Potential
Characterization
Authors: Santos Silas , Rodrigues Orlando , Campos Letícia 
Published: June 2020

Background:
Innovation mission in materials science requires new approaches to form functional materials,
wherein the concept of its formation begins in nano/micro scale. Rare earth oxides with general form (RE2O3; RE from
La to Lu, including Sc and Y) exhibit particular proprieties, being used in a vast field of applications with high
technological content since agriculture to astronomy. Despite of their applicability, there is a lack of studies on surface
chemistry of rare earth oxides. Zeta potential determination provides key parameters to form smart materials by
controlling interparticle forces, as well as their evolution during processing. This paper reports a study on zeta potential
with emphasis for rare earth oxide nanoparticles. A brief overview on rare earths, as well as zeta potential, including
sample preparation, measurement parameters, and the most common mistakes during this evaluation are reported.

Methods:
A brief overview on rare earths, including zeta potential, and interparticle forces are presented. A practical
study on zeta potential of rare earth oxides - RE2O3 (RE as Y, Dy, Tm, Eu, and Ce) in aqueous media is reported.
Moreover, sample preparation, measurement parameters, and common mistakes during this evaluation are discussed.

Results:
Potential zeta values depend on particle characteristics such as size, shape, density, and surface area. Besides,
preparation of samples which involves electrolyte concentration and time for homogenization of suspensions are
extremely valuable to get suitable results.

Conclusion:
Zeta potential evaluation provides key parameters to produce smart materials seeing that interparticle forces
can be controlled. Even though zeta potential characterization is mature, investigations on rare earth oxides are very
scarce. Therefore, this innovative paper is a valuable contribution on this field.