bluuebunny/crossref_metadata_2025_split huggingface dataset:
miR-100-5p Downregulates mTOR to Suppress the Proliferation, Migration, and Invasion of Prostate Cancer Cells
Authors: Ye Yun , Li Su-Liang , Wang Jian-Jun 
Published: December 2020

BackgroundPrevious studies have shown that miR-100-5p expression is abnormal in prostate cancer. However, the role and regulatory mechanism of miR-100-5p requires further investigation. Thus, the aim of this study was to observe the effects of miR-100-5p on the proliferation, migration and invasion of prostate cancer (PCa) cells and to explore the potential related regulatory mechanism.Materials and MethodsDifferential miRNA expression analysis was performed using next-generation sequencing (NGS) in the patients with PCa and benign prostatic hyperplasia (BPH). The expression levels of miR-100-5p were detected using real-time fluorescence quantitative PCR (qRT-PCR). PCa cells were transfected with NC-mimics or miR-100-5p mimics, inhibitor by using liposome transfection. Moreover, the CCK-8 proliferation assay, colony formation assay, cell scratch assay and Transwell assay were used to detect the effects of miR-100-5p on cell proliferation, migration, and invasion. In addition, the target gene of miR-100-5p was verified by luciferase reporter gene assay, and the influence of miR-100-5p on the expression of mTOR mRNA by qRT-PCR and the expression of mammalian target of rapamycin (mTOR) protein was detected by western blot and immunohistochemical staining.ResultsDifferential expression analysis of high-throughput sequencing data showed low expression of miR-100-5p in the patients of PCa. It was further confirmed by qRT-PCR that the expression of miR-100-5p in PCa cells was significantly lower than that in RWPE-1 cells (P&lt;0.01). miR-100-5p expression in lymph node carcinoma of prostate(LNCaP) cells was markedly upregulated after transfection with miR-100-5p mimics (P&lt;0.01), while cell proliferation, migration and invasion capacities were clearly reduced (P&lt;0.01). mTOR mRNA and protein expression was also substantially lowered (P&lt;0.01) and mTOR adjusted the expression of NOX4. Finally, we further confirmed by immunohistochemical staining that miR-100-5p regulated the expression of mTOR and NOX4.ConclusionmiR-100-5p is expressed at low levels in PCa cells, and it can suppress PCa cell proliferation, migration and invasion, the mechanism of which is related to downregulating the expression of mTOR.
Clinical Study of Using Biometrics to Identify Patient and Procedure
Authors: Sohn Jason W. , Kim Haksoo , Park Samuel B. , Lee Soyoung , Monroe James I. , Malone Thomas B. , Kinsella Timothy , Yao Min , Kunos Charles , Lo Simon S. , Shenk Robert , Machtay Mitchell 
Published: December 2020

PurposeTo reduce patient and procedure identification errors by human interactions in radiotherapy delivery and surgery, a Biometric Automated Patient and Procedure Identification System (BAPPIS) was developed. BAPPIS is a patient identification and treatment procedure verification system using fingerprints.MethodsThe system was developed using C++, the Microsoft Foundation Class Library, the Oracle database system, and a fingerprint scanner. To register a patient, the BAPPIS system requires three steps: capturing a photograph using a web camera for photo identification, taking at least two fingerprints, and recording other specific patient information including name, date of birth, allergies, etc. To identify a patient, the BAPPIS reads a fingerprint, identifies the patient, verifies with a second fingerprint to confirm when multiple patients have same fingerprint features, and connects to the patient’s record in electronic medical record (EMR) systems. To validate the system, 143 and 21 patients ranging from 36 to 98 years of ages were recruited from radiotherapy and breast surgery, respectively. The registration process for surgery patients includes an additional module, which has a 3D patient model. A surgeon could mark ‘O’ on the model and save a snap shot of patient in the preparation room. In the surgery room, a webcam displayed the patient’s real-time image next to the 3D model. This may prevent a possible surgical mistake.Results1,271 (96.9%) of 1,311 fingerprints were verified by BAPPIS using patients’ 2nd fingerprints from 143 patients as the system designed. A false positive recognition was not reported. The 96.9% completion ratio is because the operator did not verify with another fingerprint after identifying the first fingerprint. The reason may be due to lack of training at the beginning of the study.ConclusionWe successfully demonstrated the use of BAPPIS to correctly identify and recall patient’s record in EMR. BAPPIS may significantly reduce errors by limiting the number of non-automated steps.
Clinical Psychological Figures in Healthcare Professionals: Resilience and Maladjustment as the “Cost of Care”
Authors: Merlo Emanuele Maria , Stoian Anca Pantea , Motofei Ion G. , Settineri Salvatore 
Published: December 2020

Background: The health professionals are involved in the paths of care for patients with different medical conditions. Their life is frequently characterized by psychopathological outcomes so that it is possible to identify consistent burdens. Besides the possibility to develop pathological outcomes, some protective factors such as resilience play a fundamental role in facilitating the adaptation process and the management of maladaptive patterns. Personal characteristics and specific indexes such as burdens and resilience are essential variables useful to study in-depth ongoing conditions and possible interventions. The study was aimed at highlighting the presence and the relations among factors as personal variables, burdens, and resilience, to understand health professionals' specific structure and functions.Methods: The observation group was composed of 210 participants, 55 males (26.2%), and 155 females (73.8%), aged from 18 to 30 years old with a mean age of 25.92 years old (SD = 3.33). The study considered personal characteristics of the subjects, such as age, gender, years of study, days of work per week, hours of work per week, and years of work. Our study had been conducted with the use of measures related to burdens (Caregivers Burden Inventory) and resilience (Resilience Scale for Adults).Results: The performed analyses consisted of descriptive statistics, correlations, and regressions among the considered variables. Several significant correlations emerged among personal characteristics, CBI, and RSA variables. Specifically, age and work commitment indexes appeared to be significantly related to the development of burdens, differently from the years of study. Significant correlations emerged among personal and RSA variables, indicating precise directions for both domains. Age and gender were identified as predictors to perform multivariate regression analyses concerning CBI factors. Significant dependence relations emerged with reference to all CBI variables.Conclusion: Pathological outcomes and resilience factors represent two sides of the health professionals' experiences, also known as “invisible patients.” Greater knowledge about present conditions and future possibilities is a well-known need in literature so that the current analyses considered fundamental factors. In line with state of the art, future studies are needed in order to deepen elusive phenomena underlying maladjustment.
Les Sociétés Populaires comtadines : un test dans l’étude des options collectives
Authors: Lapied Martine 
Published: December 2020

The popular societies in Comtat : a test for a study of collective choices.
This work takes place into an attempt to identify politic temperaments and their formation during the french Revolution in the Comtat area, where opposite areas take shape — High, Middle, and Low Comtat, but also the Rhone region — making up a total example, remarkable by the density of its popular societies (83 % of the communes have a society in the year II). One notices too the precocity of foundations, from 1790 to the summer 1791, then from April to December 1792, which gives comparatively less importance to the terrorist period. Different rythms, contrasting attitudes lead to a variative typology of popular societies, echoing to different choices (sometimes unstable, as in Avignon) which they reflect
La dissolution des Sociétés Populaires parisiennes au printemps de l’an II
Authors: Monnier Raymonde 
Published: December 2020

The dissolution of the Parisian Popular Societies in the spring of the year II.
This work considers the ground and ways of dissolution of most popular societies in Paris between the end of germinal and prairial year II. It analyses the debates which go on with the first assaults, during the struggle of factions, before germinal. After the Hebertist’s fall, the progress of self-dissolution starts : studying several significant instances, the author brings out the counter-revolutionary intrigues of moderates, who attempt to take control of societies. The return of «honnêtes gens», giving a substance to charges of federalism, makes the patriots more sensitive to jacobin pressure for a suspension of popular societies. They yield to the need of unity, required by the persistence of interior counter-revolution to rely on the Revolutionary Government.
La symbolique des Trois Ordres réunis, dans la médaille révolutionnaire
Authors: Benzaken Jean-Charles 
Published: December 2020

Symbolics of the Three Orders gathered in the revolutionary medal.
Through the numismatic production of the first years of the Revolution, the article follows a theme which was then rather fashionable in this sphere as well as in iconography : symbolics of the Three Orders, as one can follow it from the lower and humble position of the Third Estate, bearing as Atlas the weight of kingdom, to the stages of its emancipation, marked by the great days of 1789. Portrayed individuals or emblematic objects get organised into the dialectic game of the three components, face to face or gathered.
Suspicion et Terreur dans le district de Châtillon-sur-Indre (31 juillet 1790-13 frimaire an IV)
Authors: Barlet Philippe 
Published: December 2020

L'auteur, étudiant la notion de suspicion dans le cadre d’un district berrichon distingue deux «terreurs», celle de l'an II, essentiellement politique, dirigée contre les élites urbaines liées à l'Ancien Régime, et celle de l'an III (Vendémiaire-Ventôse), économique et qui frappe les paysans par un ensemble de mesures d'intimidation destinées à leur faire livrer leur grains.
Il souligne la cohésion sociale et la stabilité du personnel administratif, où la bourgeoisie urbaine se maintient au pouvoir pendant toute la période. La rupture accrue avec le monde paysan provoque en l’an III la «Vendée de Palluau». Globalement, l’impact de la Terreur dans ce district rural semble très limité (216 arrestations).
Républiques et monarchies à l’époque révolutionnaire : une diplomatie nouvelle ?
Authors: Rao Anna Maria 
Published: December 2020

Monarchies et républiques peuvent-elles coexister ou la guerre est-elle inévitable entre elles ? La République française, fondée sur la souveraineté du peuple, doit-elle respecter les traités signés par les despotes, ou doit-elle soutenir et même encourager l’expression de la volonté populaire contre la tyrannie ? Mais d’autre part les peuples soumis au despotisme monarchique sont-ils dignes de la liberté ? Ces questions animèrent constamment les rapports entre la France et l’Italie entre 1796 et 1800.
Pour la France du Directoire, la révolution étant terminée, il fallait rassurer l’Europe : des républiques et des rois pouvaient s’allier pour des intérêts communs. Pour les patriotes italiens comme pour les néo-jacobins français, la révolution n’étant pas terminée, la France ne devait pas chercher ses amis dans les gouvernements mais dans les peuples ; à partir de 1800, les patriotes italiens eux-mêmes se rallièrent à l’idée d’une coexistence entre les régimes républicains du nord de la péninsule et les régimes monarchiques du sud.
Morphologic Analysis of Urinary Podocytes in Focal Segmental Glomerulosclerosis
Authors: Shirai Yoko , Miura Kenichiro , Yokoyama Takashi , Horita Shigeru , Nakayama Hideki , Seino Hiroshi , Ando Taro , Shiratori Atsutoshi , Yabuuchi Tomoo , Kaneko Naoto , Ishiwa Sho , Ishizuka Kiyonobu , Hara Masanori , Hattori Motoshi 
Published: December 2020

Background
The development of glomerulosclerosis in FSGS is associated with a reduction in podocyte number in the glomerular capillary tufts. Although it has been reported that the number of urinary podocytes in FSGS exceeds that of minimal-change nephrotic syndrome, the nature of events that promote podocyte detachment in FSGS remains elusive.

Methods
In this study, we provide detailed, morphologic analysis of the urinary podocytes found in FSGS by examining the size of the urinary podocytes from patients with FSGS, minimal-change nephrotic syndrome, and GN. In addition, in urinary podocytes from patients with FSGS and minimal-change nephrotic syndrome, we analyzed podocyte hypertrophy and mitotic catastrophe using immunostaining of p21 and phospho-ribosomal protein S6.

Results
The size of the urinary podocytes was strikingly larger in samples obtained from patients with FSGS compared with those with minimal-change nephrotic syndrome and GN (P=0.008). Urinary podocytes from patients with FSGS had a higher frequency of positive immunostaining for p21 (P<0.001) and phospho-ribosomal protein S6 (P=0.02) than those from patients with minimal-change nephrotic syndrome. Characteristic features of mitotic catastrophe were more commonly observed in FSGS than in minimal-change nephrotic syndrome urinary samples (P=0.001).

Conclusions
We posit that the significant increase in the size of urinary podocytes in FSGS, compared with those in minimal-change nephrotic syndrome, may be explained by hypertrophy and mitotic catastrophe.
Nahid Sırrı Örik’in “Eve Düşen Yıldırım” Hikâyesini Tahlil Denemesi
Authors: AFACAN Tuğba 
Published: December 2020

Yaşadığı dönemde herhangi bir edebî topluluğa katılmayan Nahid Sırrı Örik, özellikle roman ve hikâyeleriyle adını günümüze taşıyan yazarlardan birisidir. Yazarın “Eve Düşen Yıldırım” adlı hikâyesinin incelendiği makale, başlıca beş bölümden oluşmaktadır. Giriş bölümünde öncelikle yazarla ilgili kısa bir bilgi verildikten sonra hikâyenin künyesine ve hikâye hakkında çıkan tartışmalara değinilmiştir. Konu bölümünde hikâyenin muhtevası ayrıntılı bir şekilde anlatılmış, tema başlığında ferdî ve sosyal temaların birlikte kullanıldığı bilgisine ulaşılmıştır. Yapı bölümü; olay örgüsü, şahıs kadrosu, zaman, mekân, dil ve anlatım olmak üzere beş alt başlıkta ayrıntılı olarak değerlendirilmiştir. Olay örgüsünde hikâyenin gerilim noktalarını oluşturan belli başlı olaylar ele alınarak kahramanlar arasındaki çatışma unsurları ortaya konulmuştur. Şahıslar ise öncelikle fiziki özellikleri ile tanıtılmış ve sonrasında şahısların hikâyedeki ayırıcı niteliklerine yer verilmiştir. Zaman bahsinde hikâyenin vaka zamanı ve sosyal zamanı belirlenmiştir. Hikâyenin dış mekânı Ankara’dır ve bunun yanında Cebeci’deki ev ise iç mekânı oluşturur. Son olarak dil ve anlatım bahsinde de yazarın üslubu ve hikâyede kullanılan temel anlatım tarzları örnekler verilerek kısaca açıklanmıştır. Sonuç bölümünde ise yazarın güzellik tutkusuna vurgu yapılarak “Eve Düşen Yıldırım”ın yazarının edebî anlayışını yansıtan tipik bir hikâye örneği olduğu bulgusu saptanmıştır.
PENINGKATAN KETERAMPILAN MOTORIK HALUS MELALUI PERMAINAN SAINS BILLON PADA KELOMPOK B
Authors: Sastra Miharja Revalina Rizky Rinjany 
Published: December 2020

Peningkatan keterampilan motorik halus melalui permainan sains billon pada kelompok B TK Al Munawaroh Banjarsari. Penelitian ini dilatarbelakangi hasil pengamatan dan pengalaman peneliti bahwa kemampuan motorik halus pada anak kelompok B TK Al Munawaroh Banjarsari masih rendah. Dengan keterbatasan media yang ada di sekolah, kegiatan pembelajaran yang monoton, serta kurangnya stimulus yang dilakukan karena lembaga lebih mengutamakan calistung untuk peserta didiknya, dalam peningkatan keterampilan motorik halus menjadi kurang maksimal. Penelitian ini menggunakan metode single case experimental dengan partisipan empat orang anak berusia lima sampai enam tahun berjenis kelamin laki-laki dua orang dan berjenis kelamin perempuan dua orang yang dilibatkan dalam penelitian keterampilan motorik halus melalui permainan sains billon. Teknik pengumpulan data menggunakan lembar observasi, dan dokumentasi, sedangkan untuk teknik analisi data menggunakan statistik deskriptif. Kesimpulan hasil penelitian ini adalan penerapan permainan sains billon untuk peningkatan keterampilan motorik halus pada kelompok B TK Al Munawaroh Banjarsari. Kemampuan keterampilan motorik halus anak sebelum diberi intervesi belum berkembang (BB), pada intervensi 1 mulai berkembang (MB), dan intervensi 2 berkembang sesuai harapan (BSH). Dari uraian tersebut dapat ditarik kesimpulan penerapan permainan sains billon dapat meningkatkan keterampilan motorik halus kelompok B TK Al Munawaroh Banjarsari.
Kata Kunci: Keterampilan Motorik Halus, Permainan Sains Billon, Anak Usia Dini
ASSESSMENT OF THE ANTIBIOTIC RESISTANCE OF BACTERIAL ISOLATES RECOVERED FROM DOG FEED SOLD IN ADO-EKITI METROPOLIS
Authors: Atere Victor 
Published: December 2020

Animal feed has been incriminated in many animal infectious diseases. This study was carried out to investigate and document the bacteriological safety of dog feed sold in Ado-Ekiti Metropolis. Five feed samples were analyzed, the bacterial load, coliform count, total Salmonella count as well as test for campylobacter was carried out on the feed samples. Pure culture of the isolates were subjected to antibiotic susceptibility test using disc diffusion method. The total bacterial count ranged between 4.41 log10CFU/g and 6.36 log10CFU/g. All feed samples had coliform ranging between 2.09 log10CFU/g and 3.93 log10CFU/g. Salmonella was only recovered from feed sample DED. Only sample DDL harbours Campylobacter sp. Other bacteria isolated from the feed were Escherichia coli, Bacillus sp, Enterobacter sp, Klebsiella sp, Staphylococcus sp and Lactobacillus sp. The antibiogram showed that nitofuratoin and ciproflocacin had the best activity where 93.10 % (54 out of 58) were susceptible to the two antibiotic. Ampicilin was most resisted with only 36.21 % (21) susceptibility. Thirty one (31) of the isolates showed resistance to 3 or more antibiotic constituting 53.44 % of the entire bacterial isolate. Antibiotic resistance pattern mostly encountered were AMP, AMO, TLY and AMP, AMO, FUR with thirteen (13) isolates each. These results showed that dog feed may not be totally safe for the pets. Based on the type of bacteria isolated and the antibiotic resistance, good manufacturing practices should be ensured by manufactures in other to reduce the rate of contamination.
La memoria andina colonial: escritores indígenas y mestizos de los siglos XVI y XVII
Authors: Herrera Villagra Alejandro 
Published: December 2020

Tres excelentes escritores, el Inca Garcilaso de la Vega, Juan de Santa Cruz Pachacuti Yamqui Salcamaygua y Felipe Guaman Poma de Ayala, consiguieron registrar un recuerdo trascendente de la antigüedad andina. Postulamos que en los Comentarios Reales [1609], la Relación de Antigüedades deste Reyno del Pirú [1613] y la Nueva Corónica y Buen Gobierno [1615], ellos construyeron una memoria del pasado reivindicado a pesar de la colonialidad de su época. Este hecho nos ha impulsado a interpretar las circunstancias en que la historia de los pueblos de los Andes durante el siglo XVI experimentó una serie de alteraciones culturales, y a explicar cómo una identidad étnica trascendió gracias a la escritura histórica.
Family Disaster Planning Untuk Meningkatkan Pengetahuan dan Sikap Disabilitas Dalam Menghadapi Bencana
Authors: Susanti Brigitta Ayu Dwi , Aprilia Eva Nurlina 
Published: December 2020

Background: The family as the smallest unit in society has a share in disaster preparedness, especially for families with disabilities, this is due to the lack of disaster management for families with disabilities. According to a 2013 UN global survey, worldwide 20% of people with disabilities can save themselves and 31% of people say they need someone who can help during a disaster. Imogiri Bantul District is the red zone that suffered the worst damage and from the results of preliminary studies disaster prepared families have not been formed in real terms. Aims of this study is to analize effect pamily disaster planning to improve knowledge and ability disability person in disaster. Methods: Quasi eksperiment pre and post test without control. Research instrument with preparedness instrument. There are 31 subjects in this research. Results: Knowledge and attitude to prepare disaster in disabilities can improve significantly p<0.005 (Wilcoxon test). Conclusion: With family disaster planning the knowledge and attitude can improve significantly to prepare disaster in disabilities and their family.
Savaş ve Propaganda: 1683 Viyana Kuşatması Üzerine Bir Değerlendirme
Authors: Çolak Songül , Aydar Metin 
Published: December 2020

Propaganda, insanları istenen şekilde düşünmeye ve davranmaya ikna etmek için tasarlanmış bir yöntemdir. Geçmişi, insanların birbirleriyle ilk iletişim kurmaya başladığı zamana dayanmaktadır. Bu doğrultuda propaganda, kişisel çıkarlara hizmet eden ve sık başvurulan bir yöntem olmuştur. Tarih boyunca insanlar, propaganda faaliyetlerinden çeşitli şekillerde yararlanmışlardır. Özellikle savaş zamanlarında etkin bir şekilde kullanılan propaganda faaliyetleri, bu süreçte insanları ikna etmek, cesaretlendirmek ve savaşı desteklemek konusunda önemli bir role hizmet etmiştir. Bu bağlamda, bir savaşın başlamasından bitmesine kadar uygulanan propaganda faaliyetleri, bu kavramla savaşın birbirinden ayrılmaz bir şekilde bağlantılı olduğunu göstermektedir.
Bu çalışma, XVII. yüzyılın son çeyreğinde ortaya çıkan, Türk ve Avrupa tarihinin en önemli siyasî hadiselerinden birisi olan 1683 Viyana Kuşatması’ndaki propaganda faaliyetlerine odaklanmaktadır. Sonuçları itibarıyla, gerek Osmanlı Devleti’ni gerekse Habsburg İmparatorluğu’yla birlikte diğer Batılı devletlerin geleceğini şekillendiren bu savaş, siyasî, sosyal ve iktisadî açıdan pek çok kez ele alınmıştır. Fakat savaşın öncesinden sonuna kadar gerçekleştirilen propaganda faaliyetleri pek ele alınmamıştır. Bu noktadan hareketle çalışma, her iki tarafın savaşın karar aşamasından sonuçlanmasına kadar geçen süreçte başvurdukları propaganda yöntemlerini ortaya koymaktadır.
Çalışmada, Sadrazam Merzifonlu Kara Mustafa Paşa’nın, çeşitli propaganda faaliyetleriyle devlet erkânını savaşa ikna etmek, ordunun moralini yüksek tutmak ve düşmanın maneviyatını kırmak gibi propaganda faaliyetlerine giriştiği görülmektedir.
WR6: A Rejuvenated Runaway Star
Authors: Gvaramadze V. V. 
Published: December 2020

Abstract
WR6 is considered as a runaway star because of its large separation from the Galactic mid-plane. In this note, we use Gaia DR2 data on WR6 and the systemic velocity of the nebula around this star to derive the space velocity of WR6 of

38.1

−
6.5

+
9.0

km

s

−
1

, confirming that this star is indeed a runaway. Possible implications of this finding are discussed.