bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Practical Guide to Performance Improvement: Change Acceleration Process and Techniques to Maintain Improvements
Authors: Dawson Anthony 
Published: December 2019

ABSTRACTPerformance improvement (PI) team members and leaders can use a variety of tools to help accelerate change and sustain performance for lasting success. Key factors for PI team members to consider for change management are the ability to work together to problem solve and the involvement of the project's champion or sponsor for the duration of the project. The PI team can use the Change Acceleration Process model, which includes the Threats & Opportunities Matrix; backward imaging; and the goals, roles, processes, and interpersonal relationships checklist to accelerate and maintain their improvement efforts. After reviewing this article, the reader should have a better understanding of how to use the Change Acceleration Process model and tools to promote teamwork, recognize external factors that may hinder improvement, and stay focused on the project goals for ultimate PI project success. This is the sixth article of a six‐part series about performance improvement.
Using a Tibetan Gong to Increase Staff Member Engagement During Time Outs
Authors: Brenckle Erica A. , Gealer Delia , Milligan Marsha 
Published: December 2019

AbstractPerioperative and procedure area nurses can encounter barriers during the time‐out process. In March 2016, a mock regulatory agency surveyor identified a gap during a time out in our cardiac catheterization laboratory. We worked with our facility's holistic nursing group to identify a solution to gain the full attention of all procedure area team members during each time out. Historically, ceremonial leaders used a gong to begin events because they thought that the sound helped participants focus on the ceremony. Because we wanted staff members to participate in a mindful practice during time outs, we decided to use a Tibetan gong to draw attention to the process. After implementing this change, staff members were more engaged during the time‐out process than they were before the change. In addition, facility leaders requested that we share our process with the staff members in the perioperative and endoscopic areas.
Evaluation of FeIIEDTA‐NO reduction by thiourea dioxide in NO removal with FeIIEDTA
Authors: He Feiqiang , Zhu Xinyu , Chen Xianchun , Ding Jianhua 
Published: December 2019

AbstractReducing FeIIEDTA‐NO is key for NO absorption with FeIIEDTA absorbent. In this work, the reducing agent thiourea dioxide (TD) was for the first time employed for reducing FeIIEDTA‐NO. The behavior and kinetics of reducing FeIIEDTA‐NO with TD had been examined with different pH value, varied TD concentrations, and various temperatures. The results investigated that FeIIEDTA‐NO reducing rate rose with pH value increasing as well as elevated temperature. The reaction order of FeIIEDTA‐NO by TD was proved to be 2 on FeIIEDTA‐NO. Meanwhile, activating energy, activating entropy, and activating enthalpy of reducing FeIIEDTA‐NO by TD were separately calculated to be 49.583 kJ mol−1, −64.956 J k−1 mol−1, and 46.943 kJ mol−1. The simultaneous reduction of FeIIIEDTA and FeIIEDTA‐NO by TD was investigated. Results showed that TD was more powerful in reducing FeIIIEDTA and FeIIEDTA‐NO compared with other reduction systems. Finally, nitric oxide removal experiment illustrates that TD can remarkably strengthen the NO removal with FeIIEDTA solution. These theoretical findings could provide constructive suggestions for industrial tail gas denitrification employing associative FeIIEDTA and TD system.
A spatio‐temporal long‐term assessment on the ecological response of reef communities in a Caribbean marine protected area
Authors: Martínez‐Rendis Abigail , Acosta‐González Gilberto , Arias‐González Jesús Ernesto 
Published: December 2019

Abstract

Coral reef biodiversity is rapidly decreasing as a result of the loss of coral cover, which modifies the structure and functioning of the ecosystem. Understanding how coral reef communities respond in space and over the long term is essential in order to implement management strategies and reduce the effects of biodiversity loss on coral reefs.
Fish, coral, and algae communities were used as indicators to evaluate changes in coral reef systems. The variation of these communities was studied in a marine protected area composed of three management zones in Cozumel Coral Reef National Park in Quintana Roo, Mexico, over a period of 11 years (2004–2014). The following parameters were monitored annually: (i) total fish density; (ii) fish trophic group densities; (iii) species richness and three fish diversity indices; (iv) relative scleractinian coral cover; and (v) relative macroalgae cover.
In the years in which coastal development, such as the construction of a marina, took place, an increase in the abundance of territorial herbivorous and planktivorous fish was observed. As the coral recovered, macrocarnivores and sessile benthic invertivores were re‐established, whereas scraper herbivores showed no changes in the period of study in any of the three management zones.
Coral cover recovery showed rapid phase‐shift reversal (phase‐shift, macroalgae dominance over coral) in the three zones. Even though the fish density and coral cover recovered, the diversity indices of each fish trophic group exhibited a reduction in the three management zones over time.
Recent Progress of Electrolyte Design for Lithium Metal Batteries
Authors: Hu Zhenglin , Li Guojie , Wang Aoxuan , Luo Jiayan , Liu Xingjiang 
Published: December 2019

AbstractLithium metal batteries (LMBs) bring a bright future for the industrialization with higher voltage and energy density than conventional lithium ion batteries (LIBs) as lithium metal offers an ultra‐high specific capacity and the lowest electrochemical potential. However, they tend to confront unstable interface with electrolytes, uncontrollable dendrite growth and notorious safety concerns, seriously blocking the practical application of lithium metal anodes. Electrolyte is always considered as the most critical factor, directly affecting the overall battery behavior. In this case, the rearrangement of active components or designing novel electrolytes become indispensable approaches and tremendous efforts are required to seek for positive solutions. Herein, we provide recent advancements in prevailing liquid, solid and gel electrolytes from the perspective of their potential application in LMBs. The key scientific issues related to enhanced performance have also been highlighted.
Improving spelling for at‐risk kindergartners through element skill frequency building
Authors: Kostewicz Douglas E. , Kubina Richard M. , Brennan Kaitlyn M. 
Published: December 2019

AbstractA student's ability to spell affects literacy outcomes. Students profit from explicit spelling instruction but may also benefit from frequency building or systematic practice. The method of frequency building leads toward effortless performance or behavioral fluency. Reaching certain frequencies of behavior produces a critical learning outcome called application. The current study focused on the effects of building element spelling behaviors for at‐risk kindergartners and the subsequent application to a compound spelling skill. Visual and quantitative analysis suggest a clear experimental effect between the attainment of performance criterion for letter sounds, letter naming, and sequencing on students' spelling behavior. A discussion of the results precedes future research directions.
Fiber fractionation to understand the effect of mechanical refining on fiber structure and resulting enzymatic digestibility of biomass
Authors: Corbett Derek B. , Knoll Charlie , Venditti Richard , Jameel Hasan , Park Sunkyu 
Published: December 2019

AbstractMechanical refining results in fiber deconstruction and modifications that enhance enzyme accessibility to carbohydrates. Further understanding of the morphological changes occurring to biomass during mechanical refining and the impacts of these changes on enzymatic digestibility is necessary to maximize yields and reduce energy consumption. Although the degree of fiber length reduction relative to fibrillation/delamination can be impacted by manipulating refining variables, mechanical refining of any type (PFI, disk, and valley beater) typically results in both phenomena. Separating the two is not straightforward. In this study, fiber fractionation based on particle size performed after mechanical refining of high‐lignin pulp was utilized to successfully elucidate the relative impact of fibrillation/delamination and fiber cutting phenomena during mechanical refining. Compositional analysis showed that fines contain significantly more lignin than larger size fractions. Enzymatic hydrolysis results indicated that within fractions of uniform fiber length, fibrillation/delamination due to mechanical refining increased enzymatic conversion by 20–30 percentage points. Changes in fiber length had little effect on digestibility for fibers longer than ~0.5 mm. However, the digestibility of the fines fractions was high for all levels of refining even with the high‐lignin content.
A pump‐free tricellular blood–brain barrier on‐a‐chip model to understand barrier property and evaluate drug response
Authors: Yu Fang , Kumar Nivasini D/O Selva , Foo Lynette C. , Ng Sum Huan , Hunziker Walter , Choudhury Deepak 
Published: December 2019

AbstractDisruption of the blood–brain barrier (BBB) leads to various neurovascular diseases. Development of therapeutics required to cross the BBB is difficult due to a lack of relevant in vitro models. We have developed a three‐dimensional (3D) microfluidic BBB chip (BBBC) to study cell interactions in the brain microvasculature and to test drug candidates of neurovascular diseases. We isolated primary brain microvascular endothelial cells (ECs), pericytes, and astrocytes from neonatal rats and cocultured them in the BBBC. To mimic the 3D in vivo BBB structure, we used type I collagen hydrogel to pattern the microchannel via viscous finger patterning technique to create a matrix. ECs, astrocytes, and pericytes were cocultured in the collagen matrix. The fluid flow in the BBBC was controlled by a pump‐free strategy utilizing gravity as driving force and resistance in a paper‐based flow resistor. The primary cells cultured in the BBBC expressed high levels of junction proteins and formed a tight endothelial barrier layer. Addition of tumor necrosis factor alpha to recapitulate neuroinflammatory conditions compromised the BBB functionality. To mitigate the neuroinflammatory stimulus, we treated the BBB model with the glucocorticoid drug dexamethasone, and observed protection of the BBB. This BBBC represents a new simple, cost‐effective, and scalable in vitro platform for validating therapeutic drugs targeting neuroinflammatory conditions.
Adopting a flipped classroom approach for teaching molar calculations to biochemistry and genetics students
Authors: Francis Nigel , Morgan Alwena , Holm Sean , Davey Ross , Bodger Owen , Dudley Ed 
Published: December 2019

AbstractThe flipped classroom is a relatively new active learning pedagogical intervention, gaining popularity as a blended learning methodology. The flipped classroom comprises two distinct parts, directed learning carried out at the student's own pace away from the classroom and an interactive, class‐based activity encouraging problem‐solving and experiential learning. This research presents a 1‐year study to measure student performance and perception toward a flipped classroom approach to teaching core biochemical calculations to first‐year undergraduate biochemistry and genetics students. A post‐task questionnaire showed an overall positive student perception with an associated significant improvement in the end of module summative assessment. These results suggest that this teaching approach offers some advantages over more traditional teaching pedagogies.
Creating Flavin Reductase Variants with Thermostable and Solvent‐Tolerant Properties by Rational‐Design Engineering
Authors: Maenpuen Somchart , Pongsupasa Vinutsada , Pensook Wiranee , Anuwan Piyanuch , Kraivisitkul Napatsorn , Pinthong Chatchadaporn , Phonbuppha Jittima , Luanloet Thikumporn , Wijma Hein J. , Fraaije Marco W. , Lawan Narin , Chaiyen Pimchai , Wongnate Thanyaporn 
Published: December 2019

AbstractWe have employed computational approaches—FireProt and FRESCO—to predict thermostable variants of the reductase component (C1) of (4‐hydroxyphenyl)acetate 3‐hydroxylase. With the additional aid of experimental results, two C1 variants, A166L and A58P, were identified as thermotolerant enzymes, with thermostability improvements of 2.6–5.6 °C and increased catalytic efficiency of 2‐ to 3.5‐fold. After heat treatment at 45 °C, both of the thermostable C1 variants remain active and generate reduced flavin mononucleotide (FMNH−) for reactions catalyzed by bacterial luciferase and by the monooxygenase C2 more efficiently than the wild type (WT). In addition to thermotolerance, the A166L and A58P variants also exhibited solvent tolerance. Molecular dynamics (MD) simulations (6 ns) at 300–500 K indicated that mutation of A166 to L and of A58 to P resulted in structural changes with increased stabilization of hydrophobic interactions, and thus in improved thermostability. Our findings demonstrated that improvements in the thermostability of C1 enzyme can lead to broad‐spectrum uses of C1 as a redox biocatalyst for future industrial applications.
Highly Loaded Mildly Edge‐Oxidized Graphene Nanosheet Dispersions for Large‐Scale Inkjet Printing of Electrochemical Sensors
Authors: Nagar Bhawna , Jović Milica , Bassetto Victor Costa , Zhu Yingdi , Pick Horst , Gómez‐Romero Pedro , Merkoçi Arben , Girault Hubert H. , Lesch Andreas 
Published: December 2019

AbstractInkjet printing of electrochemical sensors using a highly loaded mildly edge‐oxidized graphene nanosheet (EOGN) ink is presented. An ink with 30 mg/mL EOGNs is formulated in a mixture of N‐methyl pyrrolidone and propylene glycol with only 30 min of sonication. The absence of additives, such as polymeric stabilizers or surfactants, circumvents reduced electrochemical activity of coated particles and avoids harsh post‐printing conditions for additive removal. A single light pulse from a xenon flash lamp dries the printed EGON film within a fraction of a second and creates a compact electrode surface. An accurate coverage with only 30.4 μg of EOGNs per printed layer and cm2 is achieved. The EOGN films adhere well to flexible polyimide substrates in aqueous solution. Electrochemical measurements were performed using cyclic voltammetry and differential pulse voltammetry. An all inkjet‐printed three‐electrode living bacterial cell detector is prepared with EOGN working and counter electrodes and silver‐based quasi‐reference electrode. The presence of E. coli in liquid samples is recorded with four electroactive metabolic activity indicators.
Electrochemical Oxidation of Glycine with Bimetallic Nickel−Manganese Oxide Catalysts
Authors: Mohan Roopathy , Modak Arindam , Subramanian Palaniappan , Cahan Rivka , Sivakumar P. , Gedanken Aharon , Schechter Alex 
Published: December 2019

AbstractA simple template‐free hydrothermal route followed by high‐temperature (800 °C) annealing in air forms Ni–Mn bimetallic oxides, namely NiMn2O4, Ni1.5Mn1.5O4, and MnNi2O4, which are characterized by XRD, Raman, EDS, and SEM analysis. The electrocatalytic activity of these metal oxides toward the oxidation of glycine molecules in alkaline condition was studied by cyclic voltammetry and linear sweep voltammetry methods. Among other nickel manganese bimetallic oxides and monometallic oxides (Mn2O3, NiO), Ni1.5Mn1.5O4 shows excellent redox characteristics with high oxidation current density (310 μA cm−2 at 0.43 V vs. Ag/AgCl) and lower onset potential (0.22 V vs. Ag/AgCl). Additionally, Ni1.5Mn1.5O4 exhibits a moderate Tafel slope (78 mV dec−1) and is electrochemically stable, as confirmed from chronoamperometry, indicating its potential for glycine oxidation. The linear dependence of the oxidation current with glycine concentration signifies that the overall process is diffusion controlled. The electrochemical results suggest that bimetallic mixed Mn and Ni oxides are promising glycine oxidation catalysts, which may be attributed to the cooperative effect between different Ni and Mn elements.
Carbon/Inorganic Hybrid Nanoarchitectures as Carriers for Signaling Elements in Electrochemical Immunosensors: First Biosensor for the Determination of the Inflammatory and Metastatic Processes Biomarker RANK‐ligand
Authors: Valverde Alejandro , Serafín Verónica , Montero‐Calle Ana , González‐Cortés Araceli , Barderas Rodrigo , Yáñez‐Sedeño Paloma , Campuzano Susana , Pingarrón José M. 
Published: December 2019

AbstractThis work reports the development of the first integrated electrochemical immunosensor for the determination of the ligand receptor activator nuclear factor‐κB (RANKL), a biomarker that plays an important role in the regulation of bone resorption process and, as reported recently, in oncology by modulating the immune system response facilitating the tumor growth and metastatic process during cancer development. The developed platform involves a sandwich‐type immunoassay with covalent immobilization of biotinylated capture antibodies onto streptavidin/4‐aminobenzoic acid (p‐ABA) grafted screen‐printed carbon electrodes (bCAb‐Strep/p‐ABA‐SPCE) and the use of an hybrid nanomaterial composed of gold nanoparticles (AuNPs) and multi‐walled carbon nanotubes (MWCNTs) as nanocarrier of multiple detector antibody and HRP molecules. Amperometric detection with the H2O2/hydroquinone (HQ) system allowed the construction of a linear calibration plot for RANKL standards between 10.4 and 1,000 pg mL−1 with a LOD of 3.1 pg mL−1. The determination implied simple handling protocols and short assay times. The developed immunosensor was applied to the determination of RANKL in human serum of patients diagnosed with colorectal cancer (CRC) or rheumatoid arthritis (RA). Each determination required only 5 μL of undiluted serum and took less than 2 h counting since the preparation of blocked‐bCAb‐Strep/p‐ABA‐SPCE.
Synthesis of β‐Glucosides with 3‐O‐Picoloyl‐Protected Glycosyl Donors in the Presence of Excess Triflic Acid: A Mechanistic Study
Authors: Mannino Michael P. , Demchenko Alexei V. 
Published: December 2019

AbstractOur previous study showed that picoloylated donors are capable of providing excellent facial stereoselectivity through the H‐bond‐mediated aglycone delivery (HAD) pathway. Presented herein is a detailed mechanistic study of stereoselective glycosylation with 3‐O‐picoloylated glucosyl donors. While reactions of glycosyl donors equipped with the 3‐O‐benzoyl group are typically non‐stereoselective because these reactions proceed via the oxacarbenium intermediate, 3‐O‐picoloylated donors are capable of providing enhanced, but somewhat relaxed, β‐stereoselectivity by the HAD pathway. In an attempt to refine this reaction, we noticed that glycosylations are highly β‐stereoselective in the presence of NIS and stoichiometric TfOH. The HAD pathway is highly unlikely because the picoloyl nitrogen is protonated under these reaction conditions. The protonation and glycosylation were studied by low‐temperature NMR, and the intermediacy of the glycosyl triflate has been observed. This article is dedicated to broadening the scope of this reaction in application to a variety of substrates and targets.
Assembly of Water‐Soluble Sugar‐Coated AIE‐Active Fluorescent Organic Nanoparticles for the Detection of Ginsenosides Based on Carbohydrate‐Carbohydrate Interactions
Authors: Jia Li‐yan , Liu Guang‐jian , Ji Yan‐ming , Zhang Yuan , Xing Guo‐wen 
Published: December 2019

AbstractA series of water‐soluble turn–on fluorescent organic nanoparticles (FONs) were designed and assembled for the detection of ginsenosides based on carbohydrate‐carbohydrate interactions (CCIs) and aggregation‐induced emission mechanism. Especially, the tetraphenylethylene luminogen coated by four sialic acids (TPE4S) exhibited a considerably high fluorescence enhancement (32‐fold) upon addition of polyglycosylated protopanaxadiol (PPD) derivatives. It is the first time that CCIs were employed for probing amphiphilic ginsenosides effectively. Our current results provide a novel sensing strategy using water‐soluble FONs to detect widely existing saponins with important biological activities.
A topic‐centric access control model for the publish/subscribe paradigm
Authors: Xie Rongna , Shi Guozhen , Guo Yunchuan , Li Fenghua 
Published: December 2019

SummaryThe publish/subscribe paradigm provides loosely coupled and scalable communication for the Internet of Things (IoT). In this paradigm, access control is an efficient approach to guaranteeing security. However, existing access control methods are not suitable for the publish/subscribe paradigm in the sensing layer of the IoT due to their coarse‐grained controls and lack of self‐configuration. To address these problems, in this paper, we propose a topic‐centric access control model (TCAC) to realize fine‐grained authorization for the sensing layer of the IoT. First, we use topics, a fundamental concept for the publish/subscribe paradigm, as the basic access control unit to dynamically authorize access according to the attributes of devices, users, and topics. Second, an administration model for TCAC is proposed to manage these attributes and configure access policies to effectively implement user‐driven access controls. Finally, a healthcare case is used to demonstrate the security of the proposed TCAC. The results show that our model is dynamic, fine‐grained, and user driven.
Copper‐Catalyzed Huisgen 1,3‐Dipolar Cycloaddition Tailored for Phosphorothioate Oligonucleotides
Authors: Honcharenko Malgorzata , Honcharenko Dmytro , Stromberg Roger 
Published: December 2019

AbstractAn efficient method for attachment of a variety of reporter groups to oligonucleotides (ONs) is copper (I) [Cu(I)]‐catalyzed Huisgen azide‐alkyne 1,3‐dipolar cycloaddition (“click reaction”). However, in the case of ONs with phosphorothioate modifications as internucleosidic linkages (PS‐ONs), this conjugation method has to be adjusted to be compatible with the sulfur‐containing groups. The method described here is adapted for PS‐ONs, utilizes solid‐supported ONs, and implements the Cu(I) bromide dimethyl sulfide complex (CuBr × Me2S) as a mediator for the click reaction. The solid‐supported ONs can be readily transformed into “clickable ONs” by on‐line addition of an alkyne‐containing linker that subsequently can react with an azido‐containing moiety (e.g., a peptide) in the presence of CuBr × Me2S. © 2019 by John Wiley & Sons, Inc.Basic Protocol 1: Conjugation on solid supportSupport Protocol: Removal of 4,4′‐dimethoxytrityl group from amino linkerBasic Protocol 2: Removal of protecting groups and cleavage from solid supportBasic Protocol 3: HPLC purification
Chemoenzymatic Halocyclization of γ,δ‐Unsaturated Carboxylic Acids and Alcohols
Authors: Younes Sabry H. H. , Tieves Florian , Lan Dongming , Wang Yonghua , Süss Philipp , Brundiek Henrike , Wever Ron , Hollmann Frank 
Published: December 2019

AbstractInvited for this month's cover is the group of Prof. Dr. Frank Hollmann at Delft University of Technology in the Netherlands. The Front Cover shows the vanadium‐dependent haloperoxidase from the marine organism Curcuvaria inaequalis, which efficiently activates halides as hypohalites that can then initiate spontaneous halo‐lactonization and halo‐etherification reactions. The Communication itself is available at 10.1002/cssc.201902240.