bluuebunny/crossref_metadata_2025_split huggingface dataset:
The dynamics between in vitro culture and metabolism: embryonic adaptation to environmental changes
Authors: de Lima Camila Bruna , dos Santos Érika Cristina , Ispada Jéssica , Fontes Patrícia Kubo , Nogueira Marcelo Fábio Gouveia , dos Santos Charles Morphy Dias , Milazzotto Marcella Pecora 
Published: September 2020

AbstractPrevious studies have discussed the importance of an optimal range of metabolic activity during preimplantation development. To avoid factors than can trigger an undesirable trajectory, it is important to learn how nutrients and metabolites interact to help launching the correct developmental program of the embryo, and how much the in vitro culture system can impair this process. Here, using the bovine model, we describe a factorial experimental design used to investigate the biochemical and molecular signature of embryos in response to different combinations of morphological features—i.e. speed of development—and external stimuli during in vitro culture—i.e. different oxygen tensions and glucose supplementation. Our analyses demonstrate that the embryos present heterogeneous metabolic responses depending on early morphological phenotypes and the composition of their surroundings. However, despite the contribution of each single stimulus for the embryo phenotype, oxygen tension is determinant for such differences. The lower oxygen environment boosts the metabolism of embryos with faster kinetics, in particular those cultured in lower glucose concentrations.
The interaction of atoms and molecules with nanocapsules and hollow nanowires
Authors: Poteryaeva Valentina A. , Bubenchikov Michael A. , Bubenchikov Alexey Michailovich , Lun-Fu Alexandr Viktorovich 
Published: September 2020

AbstractNanoporous membranes are widely used in various fields, such as gas separation, water purification, catalytic processes, and the use of batteries in electrodes. Nowadays, hollow carbon spheres or nanowires are attracting attention of researchers and experimenters due to high adjustability of their mechanical and chemical properties. This makes it possible, among other things, to more accurately adjust permeability of membranes created from this material for various atoms and molecules, which ensures a good degree of gas separation. The mathematical simulation of gas separation via nanocapsule and hollow nanowire porous membrane is performed. Research has shown that such membranes are able to separate He/$$\text{CH}_4$$

CH
4

/$$\text{H}_2$$

H
2

/$$\text{N}_2$$

N
2

gas mixtures.
High glucose alters the DNA methylation pattern of neurodevelopment associated genes in human neural progenitor cells in vitro
Authors: Kandilya Deepika , Shyamasundar Sukanya , Singh Dhiraj Kumar , Banik Avijit , Hande Manoor Prakash , Stünkel Walter , Chong Yap Seng , Dheen S. Thameem 
Published: September 2020

AbstractMaternal diabetes alters the global epigenetic mechanisms and expression of genes involved in neural tube development in mouse embryos. Since DNA methylation is a critical epigenetic mechanism that regulates gene functions, gene-specific DNA methylation alterations were estimated in human neural progenitor cells (hNPCs) exposed to high glucose (HG) in the present study. The DNA methylation pattern of genes involved in several signalling pathways including axon guidance (SLIT1-ROBO2 pathway), and Hippo pathway (YAP and TAZ) was altered in hNPCs exposed to HG. The expression levels of SLIT1-ROBO2 pathways genes (including its effectors, SRGAP1 and CDC42) which mediates diverse cellular processes such as proliferation, neurogenesis and axon guidance, and Hippo pathway genes (YAP and TAZ) which regulates proliferation, stemness, differentiation and organ size were downregulated in hNPCs exposed to HG. A recent report suggests a possible cross-talk between SLIT1-ROBO2 and TAZ via CDC42, a mediator of actin dynamics. Consistent with this, SLIT1 knockdown downregulated the expression of its effectors and TAZ in hNPCs, suggesting that HG perturbs the cross-talk between SLIT1-ROBO2 and TAZ in hNPCs. Overall, this study demonstrates that HG epigenetically alters the SLIT1-ROBO2 and Hippo signalling pathways in hNPCs, forming the basis for neurodevelopmental disorders in offspring of diabetic pregnancy.
Glucose metabolism responds to perceived sugar intake more than actual sugar intake
Authors: Park Chanmo , Pagnini Francesco , Langer Ellen 
Published: September 2020

AbstractThe authors examine study participants who have Type 2 diabetes to determine whether cognition affects glucose levels in contrast to widely held suppositions. Thirty participants who have type 2 diabetes consume beverages that have identical ingredients but have deceptive nutrition facts labels. Blood glucose levels measured four times before and after beverage consumption show that blood glucose levels increase when participants believe the beverage has high sugar content as portrayed on the labels. Also, individual eating behaviors and nutritional satisfaction are linked to changes in blood glucose levels. The study results support the concept of anticipatory budgeting on glucose metabolism. The findings provide pressing evidence for the psychobiological model of chronic disease, suggesting that psychological intervention programs may be important for diabetes management, beyond current programs in which type 2 diabetes is managed through diet, exercise, and medications only.
Spatial mapping of the collagen distribution in human and mouse tissues by force volume atomic force microscopy
Authors: Calò Annalisa , Romin Yevgeniy , Srouji Rami , Zambirinis Constantinos P. , Fan Ning , Santella Anthony , Feng Elvin , Fujisawa Sho , Turkekul Mesruh , Huang Sharon , Simpson Amber L. , D’Angelica Michael , Jarnagin William R. , Manova-Todorova Katia 
Published: September 2020

AbstractChanges in the elastic properties of living tissues during normal development and in pathological processes are often due to modifications of the collagen component of the extracellular matrix at various length scales. Force volume AFM can precisely capture the mechanical properties of biological samples with force sensitivity and spatial resolution. The integration of AFM data with data of the molecular composition contributes to understanding the interplay between tissue biochemistry, organization and function. The detection of micrometer-size, heterogeneous domains at different elastic moduli in tissue sections by AFM has remained elusive so far, due to the lack of correlations with histological, optical and biochemical assessments. In this work, force volume AFM is used to identify collagen-enriched domains, naturally present in human and mouse tissues, by their elastic modulus. Collagen identification is obtained in a robust way and affordable timescales, through an optimal design of the sample preparation method and AFM parameters for faster scan with micrometer resolution. The choice of a separate reference sample stained for collagen allows correlating elastic modulus with collagen amount and position with high statistical significance. The proposed preparation method ensures safe handling of the tissue sections guarantees the preservation of their micromechanical characteristics over time and makes it much easier to perform correlation experiments with different biomarkers independently.
Perfluorooctanoic acid in indoor particulate matter triggers oxidative stress and inflammation in corneal and retinal cells
Authors: Tien Peng-Tai , Lin Hui-Ju , Tsai Yi-Yu , Lim Yun-Ping , Chen Chih Sheng , Chang Ching-Yao , Lin Chao-Jen , Chen Jamie Jiin-Yi , Wu Shan-Mei , Huang Yuh-Jeen , Wan Lei 
Published: September 2020

AbstractTo investigate the particle size distribution of particulate matter and the concentration of specific perfluorinated compounds in indoor dust samples from several locations. Then, we used cell-based assays to investigate the effect of perfluorinated compounds on human corneal epithelial (HCEpiC), endothelial cells (HCEC) and retinal pigment epithelial cells (RPE). Indoor dust samples were collected at five different locations and PM50–10, PM10–2.5, and PM2.5–1 were fractionized. The presence and levels of 8:2 fluorotelomer alcohol, 10:2 fluorotelomer alcohol, and perfluorooctanoic acid were detected by gas chromatography–mass spectrometry. The effect of perfluorooctanoic acid on the activation of reactive oxygen species, transepithelial resistance as well as the expression of interleukin (IL)-6 and IL-8 were determined. The basolateral media of human corneal epithelial or human corneal endothelial cells were used to treat human corneal endothelial or retinal pigment epithelial cells, respectively to indicate the potential of ocular surface inflammation may result in retinal inflammation. Among perfluorinated compounds, only perfluorooctanoic acid was detected in all indoor dust samples. Perfluorooctanoic acid had the highest concentration among all perfluorinated compounds in the samples. Exposure to perfluorooctanoic acid impaired tight junction sealing and increased the levels of reactive oxygen species in human corneal epithelial cells. In human corneal epithelial cells, secretion of IL-6 and IL-8 in both apical and basolateral media was promoted significantly by perfluorooctanoic acid treatment. Stimulation with the basolateral media from perfluorooctanoic acid-treated human corneal epithelial cells induced inflammation in human corneal endothelial cells. The treatment of retinal pigment epithelial cells with the basolateral media from stimulated human corneal endothelial cells also elicited the secretion of proinflammatory cytokines. The results indicate that perfluorooctanoic acid exposure impaired the tight junction of corneal cells and caused inflammatory reactions in the retina. Exposure of the cornea to perfluorooctanoic acid contained in particulate matter might induce oxidative stress and inflammation in the retina and represent a risk factor for age-related macular degeneration.
A cross-sectional study on risk factors for infection with Parvovirus B19 and the association with anaemia in a febrile paediatric population in Ghana
Authors: Herr Wiebke , Krumkamp Ralf , Hogan Benedikt , Dekker Denise , Gyau Kennedy , Owusu-Dabo Ellis , Sarpong Nimako , Jaeger Anna , Loag Wibke , Winter Doris , Akenten Charity Wiafe , Eibach Daniel , Fickenscher Helmut , Eis-Hübinger Anna , May Jürgen , Kreuels Benno 
Published: September 2020

AbstractParvovirus B19 (B19V) occurs globally and can cause severe anaemia. The role of co-infections with Plasmodium falciparum (P. falciparum) has been controversially discussed. The study aimed to determine prevalence and severity of B19V infection, and the effect of co-infections on the risk for anaemia. Between November 2013 and April 2015 a total of 1186 hospital visits of children with fever admitted to a hospital in Ghana were recorded. Malaria, B19V and additional diagnostics for fever causes were performed. Recent B19V infection was defined as PCR and/or IgM positivity. Risk factors for a B19V infection and for anaemia were analysed. The prevalence of anaemia was compared between children with/without B19V infection, stratified for the presence of malaria. B19V IgM/PCR was positive in 6.4% (n = 76; 40 IgM + , 30 PCR + , 6 IgM + and PCR +). Among the B19V cases 60.5% had a simultaneous P. falciparum infection. B19V IgM positivity but not PCR positivity was associated with moderate-severe anaemia (OR = 2.6; 95%-CI: 1.3–5.3; P < 0.01 vs. OR = 0.9; 95%-CI: 0.4–1.8; P = 0.70). P. falciparum and IgM positive B19V infection were independent risk factors for anaemia with no evidence of effect modification. Our data show a significant association between B19V infection, defined as IgM but not PCR positivity, and moderate-severe anaemia. A multiplicative effect of B19V and P. falciparum infection was not found.
Rheological analysis of sputum from patients with chronic bronchial diseases
Authors: Patarin Jérémy , Ghiringhelli Étienne , Darsy Guillaume , Obamba Martinien , Bochu Philippe , Camara Boubou , Quétant Sébastien , Cracowski Jean-Luc , Cracowski Claire , Robert de Saint Vincent Matthieu 
Published: September 2020

AbstractBronchial diseases are characterised by the weak efficiency of mucus transport through the lower airways, leading in some cases to the muco-obstruction of bronchi. It has been hypothesised that this loss of clearance results from alterations in the mucus rheology, which are reflected in sputum samples collected from patients, making sputum rheology a possible biophysical marker of these diseases and their evolution. However, previous rheological studies have focused on quasi-static viscoelastic (linear storage and loss moduli) properties only, which are not representative of the mucus mobilisation within the respiratory tract. In this paper, we extend this approach further, by analysing both quasi-static and some dynamic (flow point) properties, to assess their usability and relative performance in characterising several chronic bronchial diseases (asthma, chronic obstructive pulmonary disease, and cystic fibrosis) and distinguishing them from healthy subjects. We demonstrate that pathologies influence substantially the linear and flow properties. Linear moduli are weakly condition-specific and even though the corresponding ranges overlap, distinct levels can be identified. This directly relates to the specific mucus structure in each case. In contrast, the flow point is found to strongly increase in muco-obstructive diseases, which may reflect the complete failure of mucociliary clearance causing episodic obstructions. These results suggest that the analysis of quasi-static and dynamic regimes in sputum rheology is in fact useful as these regimes provide complementary markers of chronic bronchial diseases.
A link between appendectomy and gastrointestinal cancers: a large-scale population-based cohort study in Korea
Authors: Park Youn Young , Lee Kil-yong , Oh Seong Taek , Park Sang Hyun , Han Kyung Do , Lee Jaeim 
Published: September 2020

AbstractAn association between appendectomy and subsequent gastrointestinal (GI) cancer development has been postulated, although the evidence is limited and inconsistent. To provide clarification, we investigated the link between appendectomy and GI cancers in a large nationwide appendectomy cohort. This cohort was derived from the claims database of the National Health Insurance Service in South Korea and comprised 158,101 patients who had undergone appendectomy between 2007 and 2014. A comparison cohort of 474,303 subjects without appendectomy was selected after 1:3 matching by age and sex. The incidence of GI cancers after appendectomy was observed, and risk factors for GI cancers were determined by using a multivariable-adjusted proportional hazards model. Appendectomy did not significantly increase the incidence of GI cancers in the overall population (1.529 and 1.557 per 1000 person-years in the non-appendectomy and appendectomy cohorts, respectively). However, appendectomy significantly increased the incidence of GI cancers in subgroups consisting of elderly (≥ 60 years) patients (adjusted HR, 1.102; 95% confidence interval, 1.011–1.201; p = 0.028) or women (adjusted HR, 1.180; 95% confidence interval, 1.066–1.306; p = 0.001).
Integrating metabolomics and targeted gene expression to uncover potential biomarkers of fungal/oomycetes-associated disease susceptibility in grapevine
Authors: Maia Marisa , Ferreira António E. N. , Nascimento Rui , Monteiro Filipa , Traquete Francisco , Marques Ana P. , Cunha Jorge , Eiras-Dias José E. , Cordeiro Carlos , Figueiredo Andreia , Sousa Silva Marta 
Published: September 2020

AbstractVitis vinifera, one of the most cultivated fruit crops, is susceptible to several diseases particularly caused by fungus and oomycete pathogens. In contrast, other Vitis species (American, Asian) display different degrees of tolerance/resistance to these pathogens, being widely used in breeding programs to introgress resistance traits in elite V. vinifera cultivars. Secondary metabolites are important players in plant defence responses. Therefore, the characterization of the metabolic profiles associated with disease resistance and susceptibility traits in grapevine is a promising approach to identify trait-related biomarkers. In this work, the leaf metabolic composition of eleven Vitis genotypes was analysed using an untargeted metabolomics approach. A total of 190 putative metabolites were found to discriminate resistant/partial resistant from susceptible genotypes. The biological relevance of discriminative compounds was assessed by pathway analysis. Several compounds were selected as promising biomarkers and the expression of genes coding for enzymes associated with their metabolic pathways was analysed. Reference genes for these grapevine genotypes were established for normalisation of candidate gene expression. The leucoanthocyanidin reductase 2 gene (LAR2) presented a significant increase of expression in susceptible genotypes, in accordance with catechin accumulation in this analysis group. Up to our knowledge this is the first time that metabolic constitutive biomarkers are proposed, opening new insights into plant selection on breeding programs.
Targeting colony stimulating factor-1 receptor signalling to treat ectopic pregnancy
Authors: Ahmad S. Furquan , Duncan W. Colin , Campbell Lisa L. , Beaty Robyn E. , Koscielniak Magda , Collins Frances , Saunders Philippa T. K. , Horne Andrew W. 
Published: September 2020

Abstract1–2% of pregnancies are ectopic, the majority implanting in the Fallopian tube. A single, systemic dose of methotrexate, a DNA-synthesis (S phase) inhibitor, has been used since 1991 for outpatient treatment of women with stable EP. However, methotrexate has limited clinical and cost effectiveness, restricting its use to 25–30% of these women. There is an unmet need for better medical treatment for EP. Colony stimulating factor-1 (CSF-1) promotes placentation and creates a pro-inflammatory environment that is fundamental for the maintenance of a normal pregnancy. We hypothesised that CSF-1 is also involved in the placentation and maintenance of an EP. Herein, we demonstrate the immunolocalisation of the CSF-1 receptor (CSF-1R) as well as its ligand (CSF-1) in immortalised first trimester trophoblast cells. We show that a specific CSF-1R kinase inhibitor, GW2580, abolishes CSF-1 induced trophoblast cell proliferation and migration and can be cytotoxic. We then demonstrate the expression of CSF-1R and CSF-1 in the cytotrophoblast and syncytiotrophoblast within ectopic implantation sites from women with EP. Our data suggests that CSF-1 is involved in the survival and proliferation of trophoblast cells in EP. This suggests that pharmacological disruption of CSF-1/CSF-1R signaling axis could be the basis of a new therapeutic for EP.
Osteopontin regulates biomimetic calcium phosphate crystallization from disordered mineral layers covering apatite crystallites
Authors: Iline-Vul Taly , Nanda Raju , Mateos Borja , Hazan Shani , Matlahov Irina , Perelshtein Ilana , Keinan-Adamsky Keren , Althoff-Ospelt Gerhard , Konrat Robert , Goobes Gil 
Published: September 2020

AbstractDetails of apatite formation and development in bone below the nanometer scale remain enigmatic. Regulation of mineralization was shown to be governed by the activity of non-collagenous proteins with many bone diseases stemming from improper activity of these proteins. Apatite crystal growth inhibition or enhancement is thought to involve direct interaction of these proteins with exposed faces of apatite crystals. However, experimental evidence of the molecular binding events that occur and that allow these proteins to exert their functions are lacking. Moreover, recent high-resolution measurements of apatite crystallites in bone have shown that individual crystallites are covered by a persistent layer of amorphous calcium phosphate. It is therefore unclear whether non-collagenous proteins can interact with the faces of the mineral crystallites directly and what are the consequences of the presence of a disordered mineral layer to their functionality. In this work, the regulatory effect of recombinant osteopontin on biomimetic apatite is shown to produce platelet-shaped apatite crystallites with disordered layers coating them. The protein is also shown to regulate the content and properties of the disordered mineral phase (and sublayers within it). Through solid-state NMR atomic carbon-phosphorous distance measurements, the protein is shown to be located in the disordered phases, reaching out to interact with the surfaces of the crystals only through very few sidechains. These observations suggest that non-phosphorylated osteopontin acts as regulator of the coating mineral layers and exerts its effect on apatite crystal growth processes mostly from afar with a limited number of contact points with the crystal.
Anticorrosive non-crystalline coating prepared by plasma electrolytic oxidation for ship low carbon steel pipes
Authors: Ma Chunsheng , Liu Jian , Zhu Xinhe , Xue Wenbin , Yan Zhijun , Cheng Dong , Fu Jingguo , Ma Shenglin 
Published: September 2020

AbstractA corrosion-resistant non-crystalline coating was fabricated by plasma electrolytic oxidation (PEO) on Q235 low carbon steel for ship pipes. The distribution and composition of chemical elements and phases of PEO coatings were analyzed by an orthogonal experiment, and the formation mechanism of PEO coatings was discussed. The corrosion current densities and corrosion potentials were measured. The results indicated that the formation of a transition layer mainly containing Fe3O4 was crucial for achieving an excellent coating quality. Furthermore, the corrosion current density of coated steel was reduced by 78% compared with the bare steel.
Influence of Co concentration on properties of NiO film by sparking under uniform magnetic field
Authors: Tippo Posak , Thongsuwan Wiradej , Wiranwetchayan Orawan , Kumpika Tewasin , Kantarak Ekkapong , Singjai Pisith 
Published: September 2020

AbstractNickel oxide (NiO) films cover numerous electronic applications, including transparent conducting oxides and hole transport layer, because of its high transparency and wide band gap. A sparking discharge is a new and unique method for the deposition of NiO films due to non-complex operation and non-requirement of a vacuum atmosphere. Unfortunately, NiO films by the sparking method display a porous surface with inferior crystallinity. By assisting a uniform magnetic field in the sparking method, the porous and the crystallinity of NiO are improved. However, electrical properties of the NiO films deposited by this strategy are poor. In order to improve the electrical properties of NiO, a substitutional of Ni ions by Co ions is considered. In this study, we report an influence of Co concentration on properties of NiO films by sparking under a uniform magnetic field. Our results indicate that an increase in Co concentration to 0.1 M improves the crystallinity and increases a carrier concentration of NiO, resulting in a reduction of the resistivity. This consequence is in agreement with the increase in a number of higher-valence Ni3+ because of the Co2+ substituted Ni2+. Based on our research, Co-NiO film is promising materials for a transparent conductor.