bluuebunny/crossref_metadata_2025_split huggingface dataset:
Standard‐space atlas of the viscoelastic properties of the human brain
Authors: Hiscox Lucy V. , McGarry Matthew D. J. , Schwarb Hillary , Van Houten Elijah E. W. , Pohlig Ryan T. , Roberts Neil , Huesmann Graham R. , Burzynska Agnieszka Z. , Sutton Bradley P. , Hillman Charles H. , Kramer Arthur F. , Cohen Neal J. , Barbey Aron K. , Paulsen Keith D. , Johnson Curtis L. 
Published: September 2020

AbstractStandard anatomical atlases are common in neuroimaging because they facilitate data analyses and comparisons across subjects and studies. The purpose of this study was to develop a standardized human brain atlas based on the physical mechanical properties (i.e., tissue viscoelasticity) of brain tissue using magnetic resonance elastography (MRE). MRE is a phase contrast‐based MRI method that quantifies tissue viscoelasticity noninvasively and in vivo thus providing a macroscopic representation of the microstructural constituents of soft biological tissue. The development of standardized brain MRE atlases are therefore beneficial for comparing neural tissue integrity across populations. Data from a large number of healthy, young adults from multiple studies collected using common MRE acquisition and analysis protocols were assembled (N = 134; 78F/ 56 M; 18–35 years). Nonlinear image registration methods were applied to normalize viscoelastic property maps (shear stiffness, μ, and damping ratio, ξ) to the MNI152 standard structural template within the spatial coordinates of the ICBM‐152. We find that average MRE brain templates contain emerging and symmetrized anatomical detail. Leveraging the substantial amount of data assembled, we illustrate that subcortical gray matter structures, white matter tracts, and regions of the cerebral cortex exhibit differing mechanical characteristics. Moreover, we report sex differences in viscoelasticity for specific neuroanatomical structures, which has implications for understanding patterns of individual differences in health and disease. These atlases provide reference values for clinical investigations as well as novel biophysical signatures of neuroanatomy. The templates are made openly available (github.com/mechneurolab/mre134) to foster collaboration across research institutions and to support robust cross‐center comparisons.
One‐pot synthesis of novel benzimidazoles with a naphthalene moiety as antimicrobial agents and molecular docking studies
Authors: Ersan Ronak Haj , Yuksel Ahmet , Ertan‐Bolelli Tugba , Dogen Aylin , Burmaoglu Serdar , Algul Oztekin 
Published: September 2020

AbstractIn an attempt to design a greener approach for the synthesis of a potent class of antimicrobials, 1,2‐phenylenediamine derivatives were reacted with various 1/2‐carboxylic acid‐substituted naphthalene derivatives to generate a series of naphthyl‐substituted benzimidazole derivatives (11–19) using polyphosphoric acid as catalyst under microwave irradiation and conventional synthesis method. This is an eco‐friendly and swift reaction method for a synthetic approach to diverse benzimidazoles. Structures of the synthesized compounds were established on the basis of spectral data and they were screened for their antimicrobial activity. Compound18showed maximum potency against all Gram‐positive and Gram‐negative bacterial strains with a minimum inhibitory concentration (MIC) value in the range of 7.81–62.50 μg/ml. Only compound17was found to be the most active against all fungal strains with a MIC value of 15.62 μg/ml. In this study, we performed molecular docking experiments to understand the interactions between compounds17and18andE. colitopoisomerase I, and we compared the results obtained with that of 2‐(3,4‐dimethoxyphenyl)‐5‐[5‐(4‐methylpiperazin−1‐yl)‐1H‐benzimidazol‐2‐yl]‐1H‐benzimidazole (DMA). Compounds17and18demonstrated strong interactions with important active site residues, similar to DMA. As a result, the compounds obtained from this study can be used in designing new potent inhibitors ofE. colitopoisomerase I.
“You are already all you need to be”: A case illustration of compassion‐focused therapy for shame and perfectionism
Authors: Matos Marcela , Steindl Stanley R. 
Published: September 2020

AbstractThis paper presents the case of a 28‐year‐old woman diagnosed with major depressive disorder, with strong features of perfectionism, shame, and self‐criticism, treated via 12 sessions of compassion‐focused therapy (CFT). CFT is an integrative therapeutic approach that draws upon evolutionary psychology, attachment theory, and applied psychological processes from neuroscience, clinical and social psychology. The effectiveness of compassion focused approaches with perfectionism and self‐criticism across a range of clinical disorders is becoming increasingly well‐established. Given this mounting evidence, a four‐phase, 12‐session CFT treatment plan was developed for this case: (1–2) establishing the therapeutic relationship; (3–4) psychoeducation regarding the evolutionary model of compassion; (5–8) compassionate mind training and skills development; (9–11) working with perfectionism, shame, and self‐criticism. A follow‐up session focused on envisioning a compassionate future. Therapeutic process and clinical outcome will be discussed, as well as implications for using CFT in clinical practice, especially where perfectionism, shame, and self‐criticism are part of the clinical presentation.
Advanced sequence optimization for the high efficient yield of human group A rotavirus VP6 recombinant protein in Escherichia coli and its use as immunogen
Authors: Masoudi Maha , Teimoori Ali , Tabaraei Alijan , Shahbazi Majid , Divbandi Marzieh , Lorestani Nazanin , Yamchi Ahad , Nikoo Hadi Razavi 
Published: September 2020

AbstractRotavirus is the important etiological agents of infectious diarrhea among children under 5 years old. Rotaviruses are divided into 10 serogroups (A–J) and each group is based on genetic properties of major structural protein VP6. We designed a novel VP6 sequence optimization to increase the expression level of this protein. Numerous factors such as codon adaptation index, codon pair bias, and guanine‐cytosine content were adapted based on Escherichiacoli codon usage. In addition, the ribosome binding site (RBS) of pET‐15b was redesigned by the RBS calculator and the secondary structure of VP6 messenger RNA was optimized in the whole length of the coding sequence. Various factors including isopropyl beta‐
d‐thiogalactoside (IPTG) concentration, temperature, and induction time were analyzed for the optimization of the best expression in E. coli by sodium dodecyl sulfate‐polyacrylamide gel electrophoresis and western blotting. The recombinant VP6 (rVP6) protein was purified by the Ni‐sepharose and then the hyperimmune sera were generated against rVP6 in rabbits. Among three different temperatures, IPTG concentrations, and postinductions, the level of rVP6 was higher at 37°C, 1 mM of IPTG, and 8 h, respectively. Also, the high expression level of rVP6 was obtained in the insoluble aggregate form (43.8 g/L). After purification, the yield of rVP6 was 10.83 g/L. The rVP6 specific antiserum was confirmed by both immunofluorescent and western blotting. The versatile sequence optimization was the reason to produce a high level of rVP6 compared to other reports and can potentially apply to produce cheaper commercial kits to diagnose serological tests and new rotavirus vaccines.
Digging metagenomic data of pangolins revealed SARS‐CoV‐2 related viruses and other significant viruses
Authors: Yang Shixing , Shan Tongling , Xiao Yuqing , Zhang Heteng , Wang Xiaochun , Shen Quan , Wang Yan , Yao Yuxin , Liu Qi , Wang Hao , Zhang Wen 
Published: September 2020

AbstractPangolin metagenomic data obtained from public databases were used to assemble partial or complete viral genomes showing genetic relationship to severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2), Sendai virus, flavivirus, picornavirus, parvovirus, and genomovirus, respectively. Most of these virus genomes showed genomic recombination signals. Phylogeny based on the SARS‐CoV‐2‐related virus sequences assembled in this study and those recently published indicated that pangolin SARS‐CoV‐2‐related viruses were clustered into two sub‐lineages according to geographic sampling sites. These findings suggest the need for further pangolin samples, from different countries, to be collected and analyzed for coronavirus to elucidate whether pangolins are intermittent hosts for SARS‐CoV‐2.
TMT‐MS/MS proteomic analysis of the carbohydrate‐active enzymes in the fruiting body of Pleurotus tuoliensis during storage
Authors: Ye Si‐Qiang , Zou Yuan , Zheng Qian‐Wang , Liu Ying‐Li , Li Rui‐Rong , Lin Jun‐Fang , Guo Li‐Qiong 
Published: September 2020

AbstractBACKGROUNDThe fruiting body of Pleurotus tuoliensis deteriorates rapidly after harvest, causing a decline in its commercial value and a great reduction in its shelf life. According to the present research, carbohydrate‐active enzymes (CAZymes) may cause the softening, liquefaction and autolysis of mature mushrooms after harvest. To further understand the in vivo molecular mechanism of CAZymes affecting the postharvest quality of P. tuoliensis fruiting bodies, a tandem mass tags labelling combined liquid chromatography–tandem mass spectrometry (TMT‐MS/MS) proteomic analysis was performed on P. tuoliensis fruiting bodies during storage at 25 °C.RESULTSA total of 4737 proteins were identified, which had at least one unique peptide and had a confidence level above 95%. Consequently, 1307 differentially expressed proteins (DEPs) were recruited using the criteria of abundance fold change (FC) >1.5 or < 0.67 and P < 0.05. The identified proteins were annotated by dbCAN2, a meta server for automated CAZymes annotation. Subsequently, 222 CAZymes were obtained. Several CAZymes participating in the cell wall degradation process, including β‐glucosidase, glucan 1,3‐β‐glucosidase, endo‐1,3(4)‐β‐glucanase and chitinases, were significantly upregulated during storage. The protein expression level of CAZymes, such as xylanase, amylase and glucoamylase, were upregulated significantly, which may participate in the P. tuoliensis polysaccharide degradation.CONCLUSIONSThe identified CAZymes degraded the polysaccharides and lignin, destroying the cell wall structure, preventing cell wall remodeling, causing a loss of nutrients and the browning phenomenon, accelerating the deterioration of P. tuoliensis fruiting body. © 2020 Society of Chemical Industry
Military Combat, Posttraumatic Stress Disorder, and the Course of Alcohol Use Disorders in a Cohort of Australian Vietnam War Veterans
Authors: O'Toole Brian I. , Gorman Patrick , Catts Stanley V. 
Published: September 2020

AbstractThe present study examined the course of diagnosed alcohol use disorders (AUDs) in a cohort of Australian veterans of the Vietnam War (N = 388) who were assessed 22 and 36 years after returning home. Standardized interviews provided data on AUDs, posttraumatic stress disorder (PTSD), other psychiatric diagnoses, and combat exposure. Overall, 148 veterans (38.1%) had no history of alcohol‐related diagnoses, 151 veterans (38.9%) had a past AUD diagnosis that was not current at the second assessment point, and 89 veterans (22.9%) had a current AUD diagnosis at the second assessment. Less education, lower intelligence test scores, and misconduct were individual risk factors for AUDs, as were first‐interview diagnoses of PTSD, antisocial personality disorder, generalized anxiety, and dysthymia, but not depression; these variables were all nonsignificant after controlling for combat exposure and PTSD. Multinomial regression was used to assess the relative contributions of combat exposure and PTSD to the course of AUDs. Combat exposure and PTSD had different patterns of association with AUDs whereby combat exposure, but not PTSD, was associated with a history of AUDs, odds ratio (OR) = 1.02, but not with current AUDs, whereas PTSD, but not combat exposure, was associated with current AUDs, OR = 3.37. Current numbing and avoidance symptoms were associated with current AUDs, OR = 4.48. The results do not support a mutual maintenance model of PTSD and AUDs but are consistent with a self‐medication model, which suggests treatment for PTSD may have beneficial effects on AUDs.
Additional Diagnostic Value of Fusion Imaging of CEUS and First CEUS of Invisible Hepatic Lesions ≤2 cm
Authors: Zhou Yan , Wang Yandong , Wang Fengmei , Zhang Xiang , Ding Jianmin , Zhou Hongyu , Jing Xiang 
Published: September 2020

ObjectiveTo explore the clinical value of image fusion of contrast‐enhanced ultrasonography (CEUS) and contrast‐enhanced computed tomography (CECT) in the diagnosis of invisible lesions with a size ≤2 cm on conventional ultrasound imaging, and compare it with the clinical value of “first CEUS” .MethodsA total of 132 patients with 147 lesions with abnormal blood supply with a size ≤2 cm on CECT were included in this study. “first CEUS” was performed for these lesions. Then “fusion CEUS,” that is, CEUS administered after fusion of US and CECT images, was carried out. The detection rates of the “first CEUS” and “fusion CEUS” were compared. How “fusion CEUS” corrects the misdiagnosis of liver lesions on CECT was analyzed.ResultsOne hundred nine lesions considered as HCC and 38 lesions considered as benign lesions on CECT were included. The detection rates for the lesions of “first CEUS” and “fusion CEUS” were 71.4% and 96.6%, respectively (P < 0.001). Among the 147 lesions, 68 were with a diameter ≤ 1 cm. The detection rate of “first CEUS” and “fusion CEUS” were 55.9% and 95.6%, respectively (P < 0.001) for the lesions with a size ≤1 cm. “Fusion CEUS” and “first CEUS” corrected the misdiagnosis in 2 lesions on CECT.ConclusionThe “first CEUS” and “fusion CEUS” can improve the lesion conspicuity. Compared with “first CEUS,” “fusion CEUS” has a higher diagnostic ability and hence can detect most of the invisible lesions on the former.
Expression of ACE2 and TMPRSS2 Proteins in the Upper and Lower Aerodigestive Tracts of Rats: Implications on COVID 19 Infections
Authors: Sato Taku , Ueha Rumi , Goto Takao , Yamauchi Akihito , Kondo Kenji , Yamasoba Tatsuya 
Published: September 2020

ObjectivePatients with coronavirus disease 2019 (COVID‐19), caused by severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2), exhibit not only respiratory symptoms but also symptoms of chemo‐sensitive disorders. Cellular entry of SARS‐CoV‐2 depends on the binding of its spike protein to a cellular receptor named angiotensin‐converting enzyme 2 (ACE2), and the subsequent spike protein‐priming by host cell proteases, including transmembrane protease serine 2 (TMPRSS2). Thus, high expression of ACE2 and TMPRSS2 is considered to enhance the invading capacity of SARS‐CoV‐2.MethodsTo elucidate the underlying histological mechanisms of the aerodigestive disorders caused by SARS‐CoV‐2, we investigated the expression of ACE2 and TMPRSS2 proteins using immunohistochemistry, in the aerodigestive tracts of the tongue, hard palate with partial nasal tissue, larynx with hypopharynx, trachea, esophagus, and lung of rats.ResultsCo‐expression of ACE2 and TMPRSS2 proteins was observed in the taste buds of the tongue, nasal epithelium, trachea, bronchioles, and alveoli with varying degrees of expression. Remarkably, TMPRSS2 expression was more distinct in the peripheral alveoli than in the central alveoli. These results coincide with the reported clinical symptoms of COVID‐19, such as the loss of taste, loss of olfaction, and respiratory dysfunction.ConclusionsA wide range of organs have been speculated to be affected by SARS‐CoV‐2 depending on the expression levels of ACE2 and TMPRSS2. Differential distribution of TMPRSS2 in the lung indicated the COVID‐19 symptoms to possibly be exacerbated by TMPRSS2 expression. This study might provide potential clues for further investigation of the pathogenesis of COVID‐19.Level of EvidenceNA Laryngoscope, 131:E932–E939, 2021
A Narrative Review of the Anti‐Hyperglycemic and Satiating Effects of Fish Protein Hydrolysates and Their Bioactive Peptides
Authors: Sharkey Shaun J. , Harnedy‐Rothwell Pádraigín A. , Allsopp Philip J. , Hollywood Lynsey E. , FitzGerald Richard J. , O'Harte Finbarr P. M. 
Published: September 2020

AbstractPrevalence of type 2 diabetes and overweight/obesity are increasing globally. Food supplementation as a preventative option has become an attractive option in comparison to increased pharmacotherapy dependency. Hydrolysates of fish processing waste and by‐products have become particularly interesting in a climate of increased food wastage awareness and are rapidly gaining traction in food research. This review summarizes the available research so far on the potential effect of these hydrolysates on diabetes and appetite suppression. Scopus and Web of Science are searched using eight keywords (fish, hydrolysate, peptides, satiating, insulinotropic, incretin, anti‐obesity, DPP‐4 [dipeptidylpeptidase‐4/IV]) returning a total of 2549 results. Following exclusion criteria (repeated appearances, non‐fish marine sources [e.g., macroalgae], and irrelevant bioactivities [e.g., immunomodulatory, anti‐thrombotic]), 44 relevant publications are included in this review. Stimulation of hormone secretion, regulation of glucose uptake, anorexigenic potential, identified mechanisms of action, and research conducted on the most potent bioactive peptides identified within these hydrolysates are all specifically addressed. Results of this review conclude that despite wide methodological variation between studies, there is significant potential for the application of fish protein hydrolysates in the management of bodyweight and hyperglycemia.
Ultra–high‐frequency ultrasound imaging of sural nerve: A comparative study with nerve biopsy in progressive neuropathies
Authors: Puma Angela , Grecu Nicolae , Villa Luisa , Butori Catherine , Besson Thomas , Cambieri Chiara , Cavalli Michele , Azulay Nicolas , Sacconi Sabrina , Raffaelli Charles 
Published: September 2020

AbstractIntroduction: Nerve ultrasound has been used increasingly in clinical practice as a complementary test for diagnostic assessment of neuropathies, but nerve biopsy remains invaluable in certain cases. The aim of this study was to compare ultra–high‐frequency ultrasound (UHF‐US) to histologic findings in progressive polyneuropathies. Methods: Ten patients with severe, progressive neuropathies underwent ultrasound evaluation of the sural nerve before nerve biopsy. Ultrasound data were compared with histologic results in a retrospective manner. Results: Sural nerves were easily identified on UHF‐US. Nerve hyperechogenicity correlated with inflammatory infiltrates on biopsy. Nerve fascicles could be identified and measured on ultrasound in the majority of patients. Discussion: Hyperechogenicity on UHF‐US may be a marker of nerve inflammation in neuropathies. Furthermore, the UHF‐US probe allows for evaluation of sensory nerves in spite of their small size, providing valuable information on their size and on their internal structure.
Dialogical Family Guidance (dfg)—Development and implementation of an intervention for families with a child with neurodevelopmental disorders
Authors: Cavonius‐Rintahaka Diana , Aho Anna Liisa , Billstedt Eva , Gillberg Christopher 
Published: September 2020

AbstractAimTo describe the development and implementation of a Dialogical Family Guidance (DFG) intervention, aimed at families with a child with neurodevelopmental disorders (NDD).DesignThe DFG components are presented and the content of a DFG training course. Professionals' experiences after the DFG training were evaluated.MethodsDialogical Family Guidance development phases and implementation process are examined. The Revised Standards for Quality Improvement Reporting Excellence checklist (SQUIRE 2.0) was used to provide a framework for reporting new knowledge.ResultsThe DFG training course seemed to increase possibilities of a more independent role as a nurse to deliver the DFG family intervention. The project showed that the use of dialogue can be difficult for some professionals. Analysis of the questionnaire completed after DFG training reported a high level of satisfaction. DFG training offered a new approach to deliver knowledge and understanding to families using dialogue, including tailored psychoeducation and emotional and practical guidance.
Physical plasma therapy accelerates wound re‐epithelialisation and enhances extracellular matrix formation in cutaneous skin grafts
Authors: Frescaline Nadira , Duchesne Constance , Favier Maryline , Onifarasoaniaina Rachel , Guilbert Thomas , Uzan Georges , Banzet Sébastien , Rousseau Antoine , Lataillade Jean‐Jacques 
Published: September 2020

AbstractSkin grafting is a surgical method of cutaneous reconstruction, which provides volumetric replacement in wounds unable to heal by primary intention. Clinically, full‐thickness skin grafts (FTSGs) are placed in aesthetically sensitive and mechanically demanding areas such as the hands, face, and neck. Complete or partial graft failure is the primary complication associated with this surgical procedure. Strategies aimed at improving the rate of skin graft integration will reduce the incidence of graft failure. Cold atmospheric plasma (CAP) is an emerging technology offering innovative clinical applications. The aim of this study was to test the therapeutic potential of CAP to improve wound healing and skin graft integration into the recipient site. In vitro models that mimic wound healing were used to investigate the ability of CAP to enhance cellular migration, a key factor in cutaneous tissue repair. We demonstrated that CAP enhanced the migration of epidermal keratinocytes and dermal fibroblasts. This increased cellular migration was possibly induced by the low dose of reactive oxygen and nitrogen species produced by CAP. Using a mouse model of burn wound reconstructed with a full‐thickness skin graft, we showed that wounds treated with CAP healed faster than did control wounds. Immunohistochemical wound analysis showed that CAP treatment enhanced the expression of the dermal–epidermal junction components, which are vital for successful skin graft integration. CAP treatment was characterised by increased levels of Tgfbr1 mRNA and collagen I protein in vivo, suggesting enhanced wound maturity and extracellular matrix deposition. Mechanistically, we show that CAP induced the activation of the canonical SMAD‐dependent TGF‐β1 pathway in primary human dermal fibroblasts, which may explain the increased collagen I synthesis in vitro. These studies revealed that CAP improved wound repair and skin graft integration via mechanisms involving extracellular matrix formation. CAP offers a novel approach for treating cutaneous wounds and skin grafts. © 2020 The Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.