bluuebunny/crossref_metadata_2025_split huggingface dataset:
Integrated bioinformatics analysis of aberrantly methylated-differentially expressed genes and pathways in age-related macular degeneration
Authors: Shen Yinchen , Li Mo , Liu Kun , Xu Xiaoyin , Zhu Shaopin , Wang Ning , Guo Wenke , Zhao Qianqian , Lu Ping , Yu Fudong , Xu Xun 
Published: January 2020

Abstract
Background: Age-related macular degeneration (AMD) represents the leading cause of visual impairment in the aging population. The goal of this study was to identify aberrantly methylated-differentially expressed genes (MDEGs) in AMD and explore the involved pathways by integrated bioinformatic analysis. Methods: Data of expression profiling GSE29801 and methylation profiling GSE102952 were obtained from the Gene Expression Omnibus database. We analyzed differentially methylated genes and differentially expressed genes in R software. Functional enrichment and protein–protein interaction (PPI) network analysis were performed using R package and Search Tool for the Retrieval of Interacting Genes online database. Hub genes were identified using Cytoscape. Results: 827 and 592 genes showed high and low expression, respectively, in GSE29801; 4117 hyper-methylated genes and 511 hypo-methylated genes were detected in GSE102952. After overlapping, we categorized 153 genes as hyper-methylated, low-expression genes (Hyper-LGs) and 24 genes as hypo-methylated, high-expression genes (Hypo-HGs). Four Hyper-LGs ( CKB , PPP3CA , TGFB2 , SOCS2 ) overlapped with AMD risk genes in Public Health Genomics and Precision Health Knowledge Base. KEGG pathway enrichment analysis indicated Hypo-HGs were enriched in the calcium signaling pathway, whereas Hyper-LGs were enriched in sphingolipid metabolism. In GO analysis, Hypo-HGs were enriched in fibroblast migration, membrane raft, coenzyme binding, etc. Hyper-LGs were enriched in mRNA transport, nuclear speck, DNA binding, etc. In PPI networks analysis, 23 nodes and 2 edges were established from Hypo-HGs, and 151 nodes and 73 edges were established from Hyper-LGs. Hub genes ( DHX9 , MAPT , PAX6 ) showed the greatest overlap. Conclusion: This study revealed potentially aberrantly MDEGs and pathways in AMD, which may improve the understanding of this disease.
Altered synaptic ultrastructure in the prefrontal cortex of Shank3-deficient rats
Authors: Jacot-Descombes Sarah , Keshav Neha U , Dickstein Dara L. , Wicinski Bridget , Janssen William G. M. , Hiester Liam , Harony-Nicolas Hala , Buxbaum Joseph D. , Hof Patrick R. , Varghese Merina 
Published: January 2020

Abstract
Background Deletion or mutations of SHANK3 lead to Phelan-McDermid syndrome and monogenic forms of autism spectrum disorder. SHANK3 encodes its eponymous scaffolding protein at excitatory glutamatergic synapses. Altered dendritic and spine morphology in the hippocampus, cerebellum and striatum have been associated with behavioral impairments in various Shank3-deficient animal models. Given the attentional deficit reported in these animals, our study explored whether deficiency of Shank3 in a rat model alters synaptic ultrastructure in the medial prefrontal cortex. Methods We used electron microscopy to determine the density of asymmetric synapses in layer III excitatory neurons of the medial prefrontal cortex in 5 week-old Shank3-homozygous knockout ( Shank3 -KO), heterozygous ( Shank3 -Het), and wild-type (WT) rats. We also measured postsynaptic density length, postsynaptic density area, and head diameter of dendritic spines at these synapses. Results All three groups had comparable synapse density and postsynaptic density length. Spine head diameter of Shank3 -Het rats, but not Shank3 -KO, was larger than WT rats. Shank3 -Het rats had wider head diameter in non-perforated synapses compared to WT and Shank3 -KO rats. The total postsynaptic density area was significantly larger in Shank3 -Het rats compared to Shank3 -KO and WT rats. These findings represent preliminary evidence for synaptic ultrastructural alterations in the medial prefrontal cortex of rats that lack one copy of Shank3 and mimic the heterozygous loss of SHANK3 in Phelan-McDermid syndrome. Limitations The Shank3 deletion in the rat model we used does not affect all isoforms of the protein and as such, would only model the effect of the mutations resulting in loss of the N-terminus of the protein. Given the higher prevalence of ASD in males, this study focused only on synaptic ultrastructure in male Shank3 -deficient rats. Conclusions We observed increased head diameter and postsynaptic density area in rats heterozygous for Shank3 deficiency. Further investigations of the mechanisms leading to altered synaptic ultrastructure in this animal model will enable us to understand better the role that Shank3 protein plays in autism spectrum disorder and Phelan-McDermid syndrome.
Relationship Between Postoperative Lordosis Distribution Index And Adjacent Segment Disease Following L4-S1 Posterior Lumbar Interbody Fusion
Authors: Zheng Guoquan , Wang Chunguo , Wang Tianhao , Hu Wenhao , Ji Quanbo , Hu Fanqi , Li Jianrui , Chaudhary Surendra K , Song Kai , Song Diyu , Zhang Zhifa , Hao Yongyu , Wang Yao , Li Jing , Zheng Qingyuan , Zhang Xuesong , Wang Yan 
Published: January 2020

Abstract
Background. ASD is an acknowledged problem of PLIF. Many studies have been reported concerning the role of LDI in spinal biomechanics. However, few reports have been published about the impact of LDI on ASD following L4-S1 PLIF. Methods. The study enrolled 200 subjects who underwent L4-S1 PLIF for degenerative spine disease from 2009 to 2014. The average follow-up term was 84 months. Several lower lumbar parameters were measured, including lower lumbar lordosis (LLL), lumbar lordosis (LL) and LDI on the pre- and postoperative radiograph. Perioperative information, comorbidities and operative data were documented. Kaplan-Meier curves were plotted for the comparisons of ASD-free survival of 3 different kinds of postoperative LDI subgroups. Results. The incidence of ASD was found to be 8.5%. LL and LLL increased by 3.96 ° (38.71 ° vs 42.67 ° , P ＜ 0.001) and 3.60 ° (26.22 ° vs 28.82 ° , P ＜ 0.001) after lower lumbar fusion surgery, respectively. Lordosis distribution index (LDI) increased by 0.03 (0.66 vs 0.69, P=0.004) postoperatively. A significant difference(P=0.001) was observed when comparing the incidence of ASD among postoperative LDI subgroups. The Kaplan-Meier curves showed a marked difference in ASD-free survival between low and moderate LDI subgroup (Log Rank test, P=0.0012) , high and moderate LDI subgroup (Log Rank test, P=0.0005) Conclusion. Patients with abnormal postoperative LDI were statistically more likely to develop ASD than those who had normal postoperative LDI. Moreover, patients with low postoperative LDI were at greater risk for developing ASD than those with high postoperative LDI over time.
A presentation on EFECE: A new locking and compressing device with Kirschner-wire and a prelımınary study for bıomechanıcal evaluatıon of thıs system&nbsp;&nbsp;
Authors: Karadeniz emre 
Published: January 2020

Abstract
Background This study aims to refine the biomechanical properties of a new Kirschner wire locking and compressing implant called EFECE.Methods EFECE implants are 5 mm in length, cylinder-shaped wire locking and compressing implants with an 8 mm radius and a hole for insertion of 1.2 mm Kirschner wire. The EFECE implant contains two pieces that are attached to each other with threads. The top piece functions as a cap, while the second piece contains three gloves for the insertion of magnetically active three balls. The locking mechanism is applied with the help of the balls in the cone-shaped gloves. The locking strength of these implants is determined through the following methods: for group 1, distraction force is applied to the devices until failure is reached, and then maximum force and total extension at the time of failure are evaluated; for group 2, cyclic distraction forces are applied to the implants against cyclic loading, and then the presence of failure and the maximum extension in a cycle are evaluated.Results In group 1, the mean pullout strength was 920 Newton, and the mean extension was 5 mm. In group 2 there was no failure, and the mean maximum extension was 1.2 mm.Conclusions EFECE implant holding power is sufficient for fracture stabilization. Our results show that these implants can be used for fracture fixation. The characteristics associated with this procedure (compression along Kirschner wires, freedome from bone quality fixation, percutaneous surgical technique, and the use of magnets for implant removal) may change surgical daily practice in the near future.
Health Information Seeking Behavior of Breast Cancer Patients: A Qualitative Study
Authors: Ravangard Ramin , Chaman-Ara Kefayat , Bahrami Elham , bahrami mohammad amin 
Published: January 2020

Abstract
Background: Breast cancer is the most common cancer of women worldwide. This study was aimed to review systematically the current literature on the health information seeking behavior of breast cancer patients and to conduct the qualitative analysis of findings. Methods: We searched PubMed, Web of Science, Science Direct, CINHAL, Embase, Google Scholar, Cochrane database of systematic review, PSYCINO, SID and Magiran databases. A total of 60 articles included in the study. Findings of all studies were extracted, the content analysis was conducted and a series of themes and categories were identified Results: 13 themes were identified in the published studies. The related findings of all themes are presented and discussed. Conclusion: We summarized and classified all the findings of studies on the health information seeking behavior of breast cancer patients. Our classification can be used for the comprehensive examination of the breast cancer patients’ health information seeking behavior to develop improvement policies.
Hospital Utilization for Non-Traumatic Dental Conditions in Oregon from 2013 to 2015
Authors: Brow Avery , Tranby Eric P. , Boynes Sean G 
Published: January 2020

Abstract
Introduction: Poor oral health remains a national concern impacting overall health and continues to burden an already overtaxed health care system. These issues are exacerbated by differing levels of coverage nationally, particularly in those states that lack any public oral health coverage. Those with significant and immediate need are forced to seek care from alternative settings and often are not equipped to provide dental treatment. Oregon operates a unique system called Coordinated Care Organizations (CCOs) designed to link providers from across disciplines and focus on prevention and chronic disease management. Methods: Using claims data from the All-Payer, All-Claims (APAC) database, this paper evaluates claims from 2013 to 2015 for all patients presenting to an emergency department (ED) or as an inpatient for a non-traumatic dental condition (NTDC) in the state of Oregon. Results: ED visits for NTDCs increased from 2013 to 2015 with 79% of ED visits for a dental condition paid for by Medicaid, and those aged 20-34 had the highest visit rate across all 3 years. Black and American Indian or Alaskan Native patients had the highest ED utilization. Residents of rural counties were more likely to utilize the ED than urban county residents. 76% of patients visited the ED 1 time with a charge of $13,888,516. Consistent with other findings, the increased visits are likely due to expanded Medicaid coverage. Increased usage by rural residents is also supported by other studies. Conclusions: This paper continues to demonstrate the need for multi-level solutions that continue to decrease ED utilization across Oregon.
Effect of low-dose exogenous surfactant on infants with acute respiratory distress syndrome after cardiac surgery: A retrospective analysis
Authors: Zhang Rongyuan , Wang Xu , Li Shoujun , Yan Jun 
Published: January 2020

Abstract
Purpose: To evaluate the effect of low-dose exogenous surfactant therapy on infants suffering acute respiratory distress syndrome (ARDS) after cardiac surgery. Materials and methods: We conducted a retrospective case-control study of the archive data of infants diagnosed with ARDS after cardiac surgery and admitted to pediatric cardiac surgical intensive care unit (PICU). A case was defined as a patient that received surfactant and standard therapy; a control was defined as a patient that underwent standard therapy. Controls were identified by matching patients based on age(±30d), weight(±3kg), risk adjustment congenital heart surgery-1 (RACHS-1), and initial ratio of partial pressure of oxygen/fraction of inspired oxygen (PaO2/FiO2) (±10). Outcome variables namely oxygenation indices (OI), ventilation index (VI), mechanical ventilation time and PICU time were compared.Results: Forty-four patients, 22 who received surfactant (surfactant group) and 22 who did not (control group) were analyzed. Surfactant group obtained a significant improvement on OI (13.9 vs 5.62; p=0.000) and VI (42.0 vs 22.4; p=0.000) in 6 hours, while control group got no improvement on OI (13.2 vs 11.5; p=0.065) and VI (40.2 vs 36.4; p=0.100). Compared with control group, surfactant group had shorter ventilation time (133.6h vs 218.4h; p=0.000) and PICU time (10.7d vs 17.5d; p=0.001). Infants in surfactant group under 3 months benefit more from OI and VI than infants over 3 months.Conclusions: In congenital heart disease infants with post-surgery ARDS, low-dose exogenous surfactant treatment could prominently improve oxygenation and reduced mechanical ventilation time and PICU time. And the improvement of oxygenation is more effective for infants under 3 months.
A comprehensive analysis of antigen-specific autoimmune liver disease related autoantibodies in patients with multiple sclerosis
Authors: Tsouris Zisis , Liaskos Christos , Dardiotis Efthymios , Barmakoudi Maria , Scheper Thomas , Tsimourtou Vana , Meyer Wolfgang , Hadjigeorgiou George , Bogdanos Dimitrios 
Published: January 2020

Abstract
Introduction : Abnormal liver function tests are frequently seen in patients with multiple sclerosis (MS) and their origin at times is attributed to the possible co-occurrence or the de novo induction of autoimmune liver diseases (AILDAiLD), namely autoimmune hepatitis (AIH) and primary biliary cholangitis (PBC). Aim: To assess the presence of AILD-related autoantibodies in a well-defined cohort of MS patients. Material and Methods : 133 MS (93 female) patients (mean age 42.7±11.9SD years, mean duration of disease 11.2 ±7.2 years were studied. Eighty age and sex-matched healthy individuals were tested as normal controls (NCs). Autoantibody testing was performed by indirect immunofluorescence (IF) using triple tissue and HEp-2, a multiparametric line immunoassay detecting anti-LKM1(anti-CYP2D6), anti-LC1(anti-FTCD), soluble liver antigen/liver-pancreas(anti-SLA/LP), AMA-M2, and (AMA-M2-3E), PBC-specific ANA (anti-gp210, anti-sp100 and anti-PML), and ELISA for anti-F-actin SMA and anti-dsDNA antibodies. Results : The prevalence of at least one AILD-related autoabs was more frequent in MS patients compared to NCs (22.3% vs 7.25% p=0.0045). AIH-1 related anti-F-actin antibodies were present in 21 (15.8%), at relatively low titres (all but three of the SMA-VG pattern by IF); anti-dsDNA in 3 (2.3%), and anti-SLA/LP in none; AIH-2 anti-LKM1 autoantibodies in 1 (0.8 %, negative by IF), and anti-LC1 in none. PBC-specific AMA-M2 in 2 (1.5%), but negative for AMA-M2-3E and IF) and PBC-specific ANA anti-PML in 6 (4.5%), anti-sp100 in 1 (0.8 %) and anti-gp210 in 1 (0.8 %). Amongst the 30 MS patients with at least one autoab positivity, only 4 (3%) had overt AILD (2 AIH-1 and 2 PBC). Conclusions : Despite the relatively frequent presence of liver autoantibodies, tested either by IF or monospecific assays, overt disease is rather infrequent discouraging autoantibody screening strategies of MS patients in the absence of clinical suspicion.
Who Should Discuss Advance Directives With Patients With Cancer and When? A Systematic Literature Review
Authors: Boyer Pierre , Gimenez Laëtitia , Druel Vladimir , Marcaud Alicia , Rougé-Bugat Marie-Eve 
Published: January 2020

Abstract
Background Among patients with cancer, discussion about the desired care is important, because progression of the disease, particularly signs of impaired consciousness, can lead to unwanted care in situations where no advance directives (ADs) have been provided. The aim of this study was to identify the most appropriate individual and time for addressing the subject of ADs with patients with cancer.Methods A systematic literature review was carried out between September 2014 and August 2015, using the following databases or sets of databases: PubMed, Web of Science, Cochrane Library, EM Premium, and the French database Public Health Data Bank. The methodological aspects of the articles were selected and evaluated with the help of PRISMA international guidelines.Results Of the 1170 articles identified, 7 were included (6 descriptive studies, 1 interventional study). A doctor was cited as the preferred individual for discussing the subject of advance directives with the patient. In 3 studies, this was listed as a general practitioner, whereas in the other 3 studies, it was an oncologist. The most opportune time was during the absence of a serious pathology (3 studies) and at the time the disease was diagnosed (2 studies).Conclusions Our results support the goals of Claeys-Leonetti, a 2016 French law concerned with the rights of patients receiving end-of-life care, and they suggest the implementation of a consultation for discussing and drafting ADs.
POTENCY TO RECYCLE HYDRAPULPER REJECT WASTE OF PAPER MILL INTO COMPOSITE PARTICLEBOARD
Authors: Rizaluddin Andri Taufick , Setiawan Yusup 
Published: January 2020

Solid waste of paper mill hydrapulper reject (HR) has a relatively high plastic content, with around 45-49% composition of the total HR. About 99% of the plastic has the catagory of High Density Polyethylene (HDPE). HDPE is a type of Polyethylene (PE) plastic which has high recyclability and may be reformed at high temperature. This study tries to discribe the potential of producing particleboards derived from HR using a hot-press method. Molded HR was being hot-pressed at a presssure of 25 kgf/cm2for 5 – 15 minutes of residence time, with a variation of of the amount of HR from 150-500 gram, and variations in hot-press temperature of 150-180°C. The resulting product is then analyzed for parameters of moisture content, density, water absorption, thickness swelling, and internal bond compared to SNI 03-2105-2006 and JIS A 5908-2003. The results showed that most of the samples were relatively accepted to the SNI. The best results are obtained at HR weight of 250 gram with 165 and 180°C pressing temperature, and pressing time more than 10 minutes. The addition of HDPE pellets shows an increment of density and decrement of moisture content of particleboard, while the addition of MA shows some decrements of moisture content and water absoption of particleboard.
Distribution of collocational expressions from the perspective of psychological and contextual meaning
Authors: Ahmad Bestun Hassan 
Published: January 2020

This study, entitled (Distribution of collocational expressions from the perspective of psychological and contextual meaning: Proverbs as an example), uses descriptive analysis method whilst its data is gathered from literature and from native speakers.
Apart from the introduction, the study consists of two parts: The first part is devoted to introducing the formulaic expressions and proverbs. The science of psychology of language is separated from psycholinguistics by explaining the scope and aim of each of them.
The second part of the study which has two sections describes various psychological dimensions. The first section, depending on psychological literature, introduces problems and psychological disorders which can be found in proverbs. In the second section of the second part, examples of the Kurdish proverbs are listed under the titles of the problems and psychological disorders. Whenever needed, the proverbs are explained psychologically.
Finally, the results, the list of references and an Arabic and English version of the abstract of the study are given.
De Monte Albán a chinatown: montajes fallidos, mudanzas inciertas
Authors: Peimbert Duarte Alejandro José 
Published: January 2020

<p>El artículo aborda tres sitios en Mexicali, México, encadenados no solo territorialmente, sino por los hilos de la historia: el río Nuevo, los condominios Monte Albán y el barrio La Chinesca. Cada uno de estos lugares evidencia no solo la conformación de un paisaje híbrido; sino que, según se argumenta en el texto, ciertos lugares fundacionales inscritos en dicho paisaje han sido transformados por diversas formas de especulación. Así, los cambios en el territorio son definidos por una sucesión de montajes fallidos y mudanzas inciertas.</p>
EFFECTS OF DIETS WITH DIFFERENT PROTEIN LEVEL FOR COBIA (Rachycentron canadum) GROWTH IN CONTROLLED TANK
Authors: Setianingsih Lulus , Santoso Limin , Saputra Suryadi 
Published: January 2020

The feed given to cobia’s juvennile (Rachycentron canadum) still uses expensive factory-made feed to be the basis of this research. This aims of this research to examine the effect of feed formula with different protein levels on optimal growth cobia. This research method used a completely randomized design (CRD) with three treatments and three replications. The treatment in this study were different protein level commercial feed (as control/46%), and formula feed (protein P1 46% and P2 protein 48%). The parameters tested included absolute weight, daily growth rate, feed conversion ratio, protein retention, and survival rate. The data obtained were analyzed by analysis of variance and continued with the duncan test with a 95% confidence interval. The results showed that the growth of absolute weight and daily growth rates generated from each treatment were significantly different (p <0.05), K (80.30 ± 11.20c g and 1.78 ± 0.25c g/day), P1 (21.87 ± 1.95a g and 0.49 ± 0.05a g/day), P2 (37.43 ± 3.29b g and 0.83 ± 0.07b g/day).