bluuebunny/crossref_metadata_2025_split huggingface dataset:
PSX-24 Seminal characteristics or Dorper rams with different social hierarchies in northern Mexico
Authors: Contreras-Villarreal Viridiana , Véliz-Deras Francisco Gerardo , Guillen-Muñoz Juan Manuel , Arellano Fernando , Carrillo-Moreno Dalia Ivette , Gaytán Alemán Leticia R , De Santiago-Miramontes Ma de los Angeles , Angel-García Oscar 
Published: November 2020

Abstract
The aim of this study was to evaluate the possible difference in seminal characteristics of Dorper rams of either high, medium or low social ranks in northern Mexico (26º N). The male’s social status was quantified through a competitive behavioral test in a herd of 36 adult males, the aggressive interactions and their consequences were registered to calculate the success index (SI), where low hierarchy (LH) rams had a SI of 0 to 0.33, medium hierarchy (MH) rams had a SI of 0.34 to 0.66 and high hierarchy (HH) had a SI of 0.67 to 1. Afterwards, the rams were subjected to an estrogenized female (2 mg of estradiol cypionate) for teasing in order to collect a semen sample with an artificial vagina. The semen was evaluated for volume, motility and concentration. The seminal characteristics differences were analyzed using a one-way ANOVA tests and stablishing a p-value of 0.10 as significance level in the SPSS statistical package. Results are shown in table 1. In conclusion, low hierarchy Dorper rams show a statistical tendency to have a lower sperm concentration.
PSX-14 Effects of thyme or/and celery on feed utilization, growth performance and meat quality of Barki lambs
Authors: Anele Uchenna , Shaaban Mahmoud , Kholif Ahmed , El Tawab Ahmed Abd , Radwan Mohamed , Hadhoud Fatma , Khattab Mostafa , Saleh Hisham 
Published: November 2020

Abstract
The current study evaluated the effects of in-feed administration of thyme, celery or their mixture (as an alternative to salinomycin ionophore) on feed utilization and growth performance of Barki lambs. Fifty-five lambs weighing 18.5 ± 1.1 kg and about 90 ± 5 days old were stratified into 5 treatment groups and fed: (1) a control diet of concentrates and corn stover (60:40, on dry matter basis), or the control diet supplemented with (2) 15 g of thyme, (3) 15 g of celery, (4) 15 g of thyme and celery mix, or (5) 1 g of salinomycin/lamb daily for 288 days. Additives increased (P &lt; 0.05) both corn stover and total intakes, while thyme and thyme/celery mix increased nutrient digestibility (P &lt; 0.05) compared with the control treatment. Celery, thyme/celery mix and salinomycin treatments decreased serum cholesterol. Celery increased (P &lt; 0.05) final body weight, weight gain and average daily gain without affecting shrunk liveweight, hot carcass weight or dressing percent. Thyme/celery mix increased fat thickness (P &lt; 0.001), while thyme, celery and thyme/celery mix treatments increased (P = 0.001) the ribeye area. Salinomycin, celery and thyme treatments increased (P &lt; 0.05) the lean proportion and decreased the fat proportion. Thyme, celery and thyme/celery mix treatments decreased (P &lt; 0.001) carcass protein, while thyme and thyme/celery treatments increased (P = 0.001) carcass water holding capacity. Salinomycin, thyme and celery treatments decreased (P = 0.002) meat brightness score, while celery treatment increased (P &lt; 0.05) redness of meat, without affecting yellowness, chroma or hue of the meat. Results in the present showed that these natural additives are comparable to salinomycin but further studies are necessary to validate the results and their mechanism of action.
PSX-18 High-density genomic description of Russian native sheep breed of the Republic of Tyva
Authors: Deniskova Tatiana Evgenievna , Abdelmanova Alexandra , Dotsev Arsen V , Sambu-Khoo Chechena , Reyer Henry , Selionova Marina I , Fornara Margaret S , Wimmers Klaus , Brem Gottfried , Zinovieva Natalia A 
Published: November 2020

Abstract
Tuva breed is the oldest native sheep breed in Russia with more than 2000 year-history and comes in third place by population size among coarse wool sheep. Tuva sheep produce high-quality meat as well as coarse wool to make traditional clothing in the Republic of Tyva. In present study, we aimed to evaluate genetic diversity and to establish genetic links of the Tuva breed with the other Russian coarse wool sheep to understand the origin and current status of this valuable livestock species. Twenty samples of Tuva were genotyped using Ovine Infinium® HD SNP BeadChip (Illumina, CA, USA). SNP-data were processed in PLINKv1.9, R package ‘diveRsity’ and SneP software. High-density SNP-genotypes of 10 coarse wool sheep breeds were used as comparison groups. Observed heterozygosity (Ho) and allelic richness (Ar) in Tuva breed were 0.336 and 1.884, respectively, and were practically equal to mean values estimated across all analyzed sheep breeds (Ho=0.335, Ar=1.884). All coarse wool breeds showed moderate heterozygote excess which was the highest in Tuva breed (1.6%). Effective population sizes estimated for three and five generations ago for Tuva breed (Ne3 =118 and Ne5 =179) were less than those in other breeds (Ne3 = 314 and Ne5 = 441 for Edilbai or Ne3 = 300 and Ne5 = 450 for Kalmyk) but not the lowest (Ne3 = 49 and Ne5 = 72 for Buubei). Values of historical effective population size estimated for 60 generations ago for Tuva breed was 1457 that was compatible with those obtained for other breeds (Ne60 from 586 for Buubei to 2634 for Kalmyk). Despite the obvious decrease in effective population size, levels of genetic diversity are not critical that would allow to develop programs to ensure preservation and sustainable development of Tuva breed. The study was funded by RSF No. 19-16-00070.
PSX-4 Behavior of goats subjected to different social isolation treatments
Authors: Kannan Govind , Batchu Phaneendra , Kouakou Brou , Terrill Thomas H , Estrada-Reyes Zaira 
Published: November 2020

Abstract
Social isolation can be stressful to goats when they are separated from the herd for veterinary care or prior to slaughter. This experiment was conducted to determine the behavior responses in goats during isolation with or without visual contact with conspecifics. Forty-eight uncastrated male Spanish goats (8-mo old) were randomly assigned to one of four isolation (TRT) pens (1.5 x 1.5 m) with metal grill panels: (i) open grill with no visual contact with conspecifics (IO), (ii) covered panels to prevent visual contact (IC), (iii) covered panels with a 30 cm x 30 cm window on one side to allow visual contact with conspecifics (IW), or (iv) open grill to allow visual contact with conspecifics (IV, control), for 90 min (n = 12 goats/TRT). Behaviors were recorded by four trained individuals. Frequency of vocalization was recorded continuously, and moving, standing, climbing, lying, and visual contact behaviors, in addition to spatial location, were recorded at 5-min intervals, but grouped into 30-min blocks (TIME). The data were analyzed using Friedman’s Two-Way ANOVA by Ranks Test in SAS; however, unranked data means are presented. Both TRT and TIME had significant effects (P &lt; 0.01) on frequency of vocalization, with mean values of 44.1, 16.6, 4.3, and 2.1 (SEM = 4.58), respectively in IO, IC, IW, and IV groups. Vocalization was highest during the first 30-min period and decreased over time with mean frequencies of 26.9, 12.4, and 11.4 (SEM = 2.43), respectively during 30, 60, and 90 min. Frequencies of climbing and lying behaviors were the highest (P &lt; 0.01) in the IC treatment. Goats in IC group also spent more time facing the corner of the pen. The results indicate that goats with no visual contact with conspecifics spend more time vocalizing or trying to escape the pen, which may indicate distress.
PSVII-33 Inter-Animal Variability in Androstenone Transport and the Potential Effect on the Development of Boar Taint
Authors: Bone Christine , Cameron Jocelyn , Squires Jim 
Published: November 2020

Abstract
Boar taint is a meat quality issue characterized by an off-odour or off-flavour in pork caused by the accumulation of androstenone in the fat. Our previous work demonstrated that androstenone binds non-specifically to albumin in the plasma and suggested that the binding affinity of androstenone might vary between individual animals, which may affect the subsequent development of boar taint. Therefore, the purpose of this study was to characterize the binding of androstenone in the plasma of animals with high and low fat androstenone concentrations to determine the effect of androstenone binding affinity on the development of boar taint. Plasma and backfat samples were obtained from 5-month-old terminal cross [Duroc x (Landrace x Yorkshire)] (n = 8) boars. An androstenone specific enzyme-linked immunosorbent assay (ELISA) was used to determine animals with high (n = 4) or low (n = 4) fat androstenone concentrations. The plasma from each boar was incubated with radiolabeled [3H]-androstenone in the presence or absence of excess unlabeled androstenone. Excess unlabeled androstenone created competition for binding sites allowing the displacement of [3H]-androstenone from albumin to be quantified. Incubations were analyzed by a novel high-performance liquid chromatography (HPLC) method we previously developed to assess androstenone binding. Statistical analysis was conducted using a two-way ANOVA. The plasma albumin concentrations were similar across individual boars; however, the percentage of androstenone that could be displaced from albumin in boars with high fat androstenone concentrations ranged from 7.1 ± 2.4% to 21.9 ± 5.2%, and was significantly lower (P = 0.01) than the percentage of androstenone displaced in the animals with low fat androstenone concentrations. These results suggest that the binding affinity of androstenone is inversely related with fat androstenone concentrations, demonstrating for the first time that the transport of androstenone in the plasma may contribute to the development of boar taint.
PSIV-34 Program Chair Poster Pick: Validating adapted scales to measure individual and situational interest in animal science undergraduates
Authors: Erickson MaryGrace , Marks Danielle , Karcher Elizabeth , Wattiaux Michel 
Published: November 2020

Abstract
Efforts to improve the quality of teaching and learning in animal science are forestalled by the lack of psychometric scales validated in our disciplinary context. Researchers have used instruments validated outside of animal science reliably, but this approach has questionable validity. The objective of our research was to adapt and validate scales to measure the motivational variables individual interest (II) and situational interest (SI) in introductory animal sciences students. A total of 254 introductory course students in two consecutive semesters rated their interest in animal sciences on unidimensional II (8-item) and 3-factor SI (11-item) scales previously validated for psychology undergraduates. After adapting instruments with wording specific to animal sciences, we conducted a series of confirmatory factor analyses. First, we discovered and removed two problematic items from the unidimensional II scale, offered theory-based explanations for differential item functioning in animal sciences students, and validated a revised II scale (λ = 0.74 - 0.94, CFI = 0.995, RMSEA = 0.027). Next, we confirmed the validity and reliability of the SI scale and its three subscales (λ = 0.83 - 0.96, CFI = 0.979, RMSEA = 0.048). Finally, to explore the dimensionality of SI in our population, we fitted a bifactor model and computed ancillary indices. Results supported the reliability and empirical validity of the bifactor model as an alternative conceptualization of SI (CFI = 0.986, RMSEA = 0.044). and indicated that the SI scale is mostly unidimensional (ω H = 0.923). This suggests that total SI scores can be used as a composite measure but that subscale scores are substantially contaminated by the general SI factor and should not be interpreted as unique. We present the finalized scales, recommendations for their use in animal sciences classrooms, and suggestions for future research.
PSI-28 Effect of Pluronic-F68 fog solution on performance and morbidity of newly-received heifer calves
Authors: Sowers CiCi A , Gouvea Vinicius N , Barnes Michael L , Duff Glenn C 
Published: November 2020

Abstract
Morbidity and mortality from bovine respiratory disease of newly-received feedlot cattle continues to be a problem for the feedlot industry. The objective of this study was to evaluate effects of utilizing a novel breathing treatment containing a non-ionic surfactant (Pluronic-F68) on performance and morbidity of high-risk calves during a 45-d receiving period. Angus/Angus-cross heifer calves (n = 240) were acquired in Delhi, LA and transported (14h) to the research facility. Heifers were allowed 21-h rest with access to water and RAMP® prior to processing. Heifers were sorted into 16 pens by processing order and randomized into one of two treatments: novel breathing treatment containing 6.25% Pluronic-F68 solution, 28.13% glycerin, and 65.62% water (FOG; n = 8 pens/treat and 15 heifers/pen) and control (CON; n = 8 pens/treat and 15 heifers/pen). Control heifers were held in an enclosed stock trailer for 10 min and followed by FOG heifers, during which time treatment was administered. The person responsible for identifying signs of morbidity was blinded to treatment assignments. Data were analyzed as a completely randomized design using MIXED (continuous) or GLIMMIX (binomial) models of SAS 9.4. Average daily gain was similar between treatments (P = 0.91). No differences were found in dry matter intake (P = 0.14) nor in feed efficiency (P = 0.58). There were no differences (P = 0.74) in final body weights. Morbidity was similar at first, second and third antimicrobial administration regardless of treatment (P ≥ 0.34). The number of antimicrobial treatments required or the management of BRD was similar between treatments (P = 0.72) There was no difference (P = 0.44) in mortality between FOG and CON groups. The Pluronic-F68 solution did not improve performance or reduce morbidity of newly received heifer calves; however, further research with a different concentration and/or duration of fogging may be warranted.
PSI-29 Effects of maternal supplementation of ruminally undegradable protein during early gestation on subsequent progeny performance in beef cattle
Authors: Selman Alexis , Lemke Lexi , Woodbury Jordan , Rosasco Shelby L , Scholljegerdes Eric J , Beard Joslyn K , Summers Adam F 
Published: November 2020

Abstract
Late gestation protein supplementation has been shown to be beneficial to fetal development. However, little is known about early gestation protein supplementation when supplements contain high or low amounts of ruminally undegraded protein (RUP). Therefore, two-year study was conducted using 28 mixed-aged cows consumed a basal diet balanced to meet nutrient requirements for early gestation were randomly assigned to receive one of three treatments during the first trimester of pregnancy, 1) no supplement (CON; n = 10); 2) 38% CP supplement containing 36% RUP (LRUP; n = 9); 3) 38% protein supplemented containing 50% RUP (HRUP; n = 9). All cows were managed similarly after the completion of the supplementation period. There were no treatment × year interactions observed for any variable measured. Subsequent progeny birth BW did not differ between treatments (P = 0.19). Calf weights did not differ due to treatment due to treatment (P ≥ 0.08) at weaning; however, calf weaning weight was greater in year 1 compared to year 2 (P &lt; 0.0001). After weaning, calves were placed in an individual feeding system for a 60 d feeding period. Body weight was recorded biweekly and intake was measured daily. Intake from day 0 to 14 and 14 to 28 tended to be greater (P ≤ 0.07) for HRUP and LRUP when compared to unsupplemented control. Nonetheless, ADG and gain:feed was similar for all treatments (P ≥ 0.21). Steers were transitioned to a finishing diet and harvested at approximately 18 mo of age. No differences were observed in ribeye area, hot carcass weight or yield grade (P ≥ 0.23). Overall, under the conditions of this study, supplementing cows during the first trimester with a high protein supplement that varied in ruminally undegradable protein did not impact progeny growth or carcass characteristics.
PSI-38 Relationship between body condition score, claw set, and foot angle in Angus and Red Angus beef cows grazing native annual-grass rangeland in northern California
Authors: Banwarth Megan R , DeAtley Kasey , Doyle Stephen 
Published: November 2020

Abstract
Evaluating the structure of beef cows, especially when replacement females are being selected, has great emphasis on production goals, particularly longevity of the female. Two structure traits that are important when evaluating beef cattle are claw set and foot angle, yet the relationship between body condition scores (BCS), claw set, foot angle and year of age has not been extensively investigated. The objective of this study was to determine the relationship between BCS, claw set and foot angle in Angus (n = 40) and Red Angus (n = 19) beef cows grazing irrigated pasture and annual-grass rangeland in northern California. Traits were collected 44 d post-weaning and included BCS, claw set, and foot angle. Claw set and foot angle were collected using the scale of the American Angus Association (e.g., scale = 1–9). Spring-calving beef cows were under similar management. At time of trait collection cows were grazing irrigated pasture (CP: 38%, and NDF: 49.2%, on a DM basis). Data were analyzed using ANOVA and Pearson correlation. Fixed effects were claw set, foot angle and breed, and respective interactions. Body condition score was related to foot angle scores (r = 0.38; P &lt; 0.01). Claw set was related to foot angle scores (r = 0.30; P &lt; 0.05). Results showed BCS was not different among breeds, claw set, or year of age (P &gt; 0.05). No relationship was detected among BCS and claw set. Interaction terms were not significant (P &gt; 0.05). Cows with acceptable foot angle had lower BCS than unacceptable cows (5.86 ± 0.15 vs. 6.72 ± 0.21 BCS). Results indicate that BCS and foot angle are related. Further research to investigate effect of BCS on differences of foot angle is warranted.
PSI-40 Two mitochondrial lineages revealed in North American yak
Authors: Treffer Leah K , Rice Edward S , Fuller Anna M , Cutler Samuel , Petersen Jessica L 
Published: November 2020

Abstract
Domestic yak (Bos grunniens) are bovids native to the Asian Qinghai-Tibetan Plateau. Studies of Asian yak have revealed that introgression with domestic cattle has contributed to the evolution of the species. When imported to North America (NA), some hybridization with B. taurus did occur. The objective of this study was to use mitochondrial (mt) DNA sequence data to better understand the mtDNA origin of NA yak and their relationship to Asian yak and related species. The complete mtDNA sequence of 14 individuals (12 NA yak, 1 Tibetan yak, 1 Tibetan B. indicus) was generated and compared with sequences of similar species from GeneBank (B. indicus, B. grunniens (Chinese), B. taurus, B. gaurus, B. primigenius, B. frontalis, Bison bison, and Ovis aries). Individuals were aligned to the B. grunniens reference genome (ARS_UNL_BGru_maternal_1.0), which was also included in the analyses. The mtDNA genes were annotated using the ARS-UCD1.2 cattle sequence as a reference. Ten unique NA yak haplotypes were identified, which a haplotype network separated into two clusters. Variation among the NA haplotypes included 93 nonsynonymous single nucleotide polymorphisms. A maximum likelihood tree including all taxa was made using IQtree after the data were partitioned into twenty-two subgroups using PartitionFinder2. Notably, six NA yak haplotypes formed a clade with B. indicus; the other four haplotypes grouped with B. grunniens and fell as a sister clade to bison, gaur and gayal. These data demonstrate two mitochondrial origins of NA yak with genetic variation in protein coding genes. Although these data suggest yak introgression with B. indicus, it appears to date prior to importation into NA. In addition to contributing to our understanding of the species history, these results suggest the two major mtDNA haplotypes in NA yak may functionally differ. Characterization of the impact of these differences on cellular function is currently underway.
Evaluation of Potential Optical Radiation Hazards from LED Flashlights
Authors: Landry Robert J. , James Robert H. , Miller Sharon A. , Ilev Ilko K. 
Published: November 2020

Abstract
We performed optical radiation safety evaluations of LED flashlights to determine if they pose potential ocular hazards. Six commercially available flashlight samples were randomly selected from various vendors online. They were evaluated in accordance with specifications provided in the American National Standards Institute/Illuminating Engineering Society of North America (ANSI/IESNA) Standards RP 27.1 and RP 27.3. Four of the flashlights were found to have relatively high blue-light-weighted radiance values with short times (40 to 50 s) to reach the exposure limit specified in RP 27.1. These flashlights are in Risk Group 2 and present a moderate risk for retinal damage. Two of the flashlights are in Risk Group 1 and present a low risk for retinal damage. None of the flashlights present an ultraviolet (UV) radiation hazard or a retinal thermal hazard. Cautionary labeling on the packaging as required by RP 27.3 and on the flashlight handle is recommended for flashlights and on other handheld light sources that are in Risk Group 2 or Risk Group 3.
Tintelnotia destructans Keratitis: A Clinicopathological Report and Review of the Literature
Authors: Kaufmann Claude , Arnold Mihaela , Schipf Alexander , Bruderer Vera L. , Iselin Katja C. 
Published: November 2020

Purpose:
To present the first clinicopathological report of Tintelnotia destructans keratitis, a filamentous fungus and as of yet uncommon virulent ocular pathogen.

Methods:
A 70-year-old man presented with an infectious keratitis featuring a stromal infiltrate with feathery borders and a viscous hypopyon. Despite initial improvement under a combined therapy with natamycin and voriconazole, a perforation in the further course required a penetrating keratoplasty. Cultures and the corneal lenticule were available for microscopic examination and antifungal susceptibility testing. The limited literature on the subject was reviewed.

Results:
Microscopic examination of cultures revealed hyphae and conidia being produced in globose fruiting bodies, a common characteristic of Tintelnotia sp. Histopathology showed short-branched hyphae that grew across the cornea regardless of the orientation of the collagen lamellae. Molecular methods identified the species T. destructans. The pattern of antifungal susceptibility included amphotericin B, ciclopirox, natamycin, posaconazole, voriconazole, and terbinafine. The postoperative clinical course was without complications.

Conclusions:
Although the clinical signs corresponded to the classic features of fungal keratitis, microscopic analysis revealed morphological characteristics of a fungal class that has shown little ophthalmological appearance so far. Data on T. destructans keratitis are highly limited in the literature, but all identified species shared sensitivity to terbinafine.