bluuebunny/crossref_metadata_2025_split huggingface dataset:
Forces during cellular uptake of viruses and nanoparticles at the ventral side
Authors: Wiegand Tina , Fratini Marta , Frey Felix , Yserentant Klaus , Liu Yang , Weber Eva , Galior Kornelia , Ohmes Julia , Braun Felix , Herten Dirk-Peter , Boulant Steeve , Schwarz Ulrich S. , Salaita Khalid , Cavalcanti-Adam E. Ada , Spatz Joachim P. 
Published: January 2020

AbstractMany intracellular pathogens, such as mammalian reovirus, mimic extracellular matrix motifs to specifically interact with the host membrane. Whether and how cell-matrix interactions influence virus particle uptake is unknown, as it is usually studied from the dorsal side. Here we show that the forces exerted at the ventral side of adherent cells during reovirus uptake exceed the binding strength of biotin-neutravidin anchoring viruses to a biofunctionalized substrate. Analysis of virus dissociation kinetics using the Bell model revealed mean forces higher than 30 pN per virus, preferentially applied in the cell periphery where close matrix contacts form. Utilizing 100 nm-sized nanoparticles decorated with integrin adhesion motifs, we demonstrate that the uptake forces scale with the adhesion energy, while actin/myosin inhibitions strongly reduce the uptake frequency, but not uptake kinetics. We hypothesize that particle adhesion and the push by the substrate provide the main driving forces for uptake.
Sertoli cell ablation and replacement of the spermatogonial niche in mouse
Authors: Yokonishi Tetsuhiro , McKey Jennifer , Ide Shintaro , Capel Blanche 
Published: January 2020

AbstractSpermatogonia, which produce sperm throughout the male lifetime, are regulated inside a niche composed of Sertoli cells, and other testis cell types. Defects in Sertoli cells often lead to infertility, but replacement of defective cells has been limited by the inability to deplete the existing population. Here, we use an FDA-approved non-toxic drug, benzalkonium chloride (BC), to deplete testis cell types in vivo. Four days after BC administration, Sertoli cells are preferentially depleted, and can be replaced to promote spermatogenesis from surviving (host) spermatogonia. Seven days after BC treatment, multiple cell types can be engrafted from fresh or cryopreserved testicular cells, leading to complete spermatogenesis from donor cells. These methods will be valuable for investigation of niche-supporting cell interactions, have the potential to lead to a therapy for idiopathic male infertility in the clinic, and could open the door to production of sperm from other species in the mouse.
Tissue-infiltrating macrophages mediate an exosome-based metabolic reprogramming upon DNA damage
Authors: Goulielmaki Evi , Ioannidou Anna , Tsekrekou Maria , Stratigi Kalliopi , Poutakidou Ioanna K. , Gkirtzimanaki Katerina , Aivaliotis Michalis , Evangelou Konstantinos , Topalis Pantelis , Altmüller Janine , Gorgoulis Vassilis G. , Chatzinikolaou Georgia , Garinis George A. 
Published: January 2020

AbstractDNA damage and metabolic disorders are intimately linked with premature disease onset but the underlying mechanisms remain poorly understood. Here, we show that persistent DNA damage accumulation in tissue-infiltrating macrophages carrying an ERCC1-XPF DNA repair defect (Er1F/−) triggers Golgi dispersal, dilation of endoplasmic reticulum, autophagy and exosome biogenesis leading to the secretion of extracellular vesicles (EVs) in vivo and ex vivo. Macrophage-derived EVs accumulate in Er1F/− animal sera and are secreted in macrophage media after DNA damage. The Er1F/− EV cargo is taken up by recipient cells leading to an increase in insulin-independent glucose transporter levels, enhanced cellular glucose uptake, higher cellular oxygen consumption rate and greater tolerance to glucose challenge in mice. We find that high glucose in EV-targeted cells triggers pro-inflammatory stimuli via mTOR activation. This, in turn, establishes chronic inflammation and tissue pathology in mice with important ramifications for DNA repair-deficient, progeroid syndromes and aging.
Ultrafast photomechanical transduction through thermophoretic implosion
Authors: Kavokine Nikita , Zou Shuangyang , Liu Ruibin , Niguès Antoine , Zou Bingsuo , Bocquet Lydéric 
Published: January 2020

AbstractSince the historical experiments of Crookes, the direct manipulation of matter by light has been both a challenge and a source of scientific debate. Here we show that laser illumination allows to displace a vial of nanoparticle solution over centimetre-scale distances. Cantilever-based force measurements show that the movement is due to millisecond-long force spikes, which are synchronised with a sound emission. We observe that the nanoparticles undergo negative thermophoresis, and ultrafast imaging reveals that the force spikes are followed by the explosive growth of a bubble in the solution. We propose a mechanism accounting for the propulsion based on a thermophoretic instability of the nanoparticle cloud, analogous to the Jeans’s instability that occurs in gravitational systems. Our experiments demonstrate a new type of laser propulsion and a remarkably violent actuation of soft matter, reminiscent of the strategy used by certain plants to propel their spores.
The accumulation profiles of terpene metabolites in three Muscat table grape cultivars through HS-SPME-GCMS
Authors: Sun Lei , Zhu Baoqing , Zhang Xuanyin , Wang Huiling , Yan Ailing , Zhang Guojun , Wang Xiaoyue , Xu Haiying 
Published: January 2020

AbstractAroma is an important parameter for table grapes and wines; terpenes are typical compounds in Muscat-type grape cultivars and can be easily perceived by humans because of their low olfactory threshold. Volatile terpenes contribute directly to the aroma character, while glycoside-bound terpenes are potential aromatic compounds and can be changed to their volatile forms via hydrolysis. With gas chromatography-mass spectrometry and a solid-phase microextraction method, an automatic data analysis platform was constructed; terpene compounds were identified and quantified from three table grape cultivars at three stages during berry development, and the raw data were deposited in MetaboLights. Terpene metabolite accumulation profiles are presented in this article for integrative analysis with the transcriptome data and phenotypic data to elucidate the important candidate genes and mechanism for terpene biosynthesis. Our method has applications in the identification and quantification of terpene compounds with very low or trace concentrations.
Conditional deletion of E11/Podoplanin in bone protects against ovariectomy-induced increases in osteoclast formation and activity
Authors: Staines Katherine A. , Hopkinson Mark , Dillon Scott , Stephen Louise A. , Fleming Robert , Sophocleous Antonia , Buttle David J. , Pitsillides Andrew A. , Farquharson Colin 
Published: January 2020

Abstract
E11/Podoplanin (Pdpn) is implicated in early osteocytogenesis and the formation of osteocyte dendrites. This dendritic network is critical for bone modelling/remodelling, through the production of receptor activator of nuclear factor κ B (RANK)-ligand (RANKL). Despite this, the role of Pdpn in the control of bone remodelling is yet to be established in vivo. Here we utilised bone-specific Pdpn conditional knockout mice (cKO) to examine the role of Pdpn in the bone loss associated with ovariectomy (OVX). MicroCT revealed that Pdpn deletion had no significant effect on OVX-induced changes in trabecular microarchitecture. Significant differences between genotypes were observed in the trabecular pattern factor (P&lt;0.01) and structure model index (P&lt;0.01). Phalloidin staining of F-actin revealed OVX to induce alterations in osteocyte morphology in both wild-type (WT) and cKO mice. Histological analysis revealed an expected significant increase in osteoclast number in WT mice (P&lt;0.01, compared with sham). However, cKO mice were protected against such increases in osteoclast number. Consistent with this, serum levels of the bone resorption marker Ctx were significantly increased in WT mice following OVX (P&lt;0.05), but were unmodified by OVX in cKO mice. Gene expression of the bone remodelling markers Rank, Rankl, Opg and Sost were unaffected by Pdpn deletion. Together, our data suggest that an intact osteocyte dendritic network is required for sustaining osteoclast formation and activity in the oestrogen-depleted state, through mechanisms potentially independent of RANKL expression. This work will enable a greater understanding of the role of osteocytes in bone loss induced by oestrogen deprivation.
Survival and prognostic factors of patients with esophageal fistula in advanced esophageal squamous cell carcinoma
Authors: Guan Xin , Liu Chao , Zhou Tianshuo , Ma Zhigang , Zhang Chunhui , Wang Bojun , Yao Yang , Fan Xiaona , Li Zhiwei , Zhang Yanqiao 
Published: January 2020

Abstract
The aim of the present study was to investigate the survival and prognostic factors of patients who were with advanced esophageal squamous cell carcinoma (ESCC) and developed an esophageal fistula. The data from 221 patients with advanced ESCC developed esophageal fistula from January 2008 to December 2017 at the Harbin Medical University Cancer Hospital was retrospectively analyzed. Hazard ratios (HRs) and 95% confidence intervals (CIs) were estimated by the Cox proportional hazard models. The median survival time after a diagnosis of the esophageal fistula was calculated using the Kaplan–Meier method. We found that the pathogens infected by patients are common bacteria in nosocomial infection. Besides, the incidence rate of esophagomediastinal fistula was the highest (54.2%) in the lower third of the esophagus. Kaplan–Meier analysis revealed a median survival time of 11.00 months and a median post-fistula survival time of 3.63 months in patients who developed esophageal fistula in advanced esophageal cancer. In the univariate analysis, gender, therapies for ESCC before the development of fistula, type of esophageal fistula, treatment of esophageal fistula and hemoglobin (Hb) level were the factors with significant prognostic value. Gender, type of esophageal fistula and Hb level were identified as independent prognostic factors in further multivariate analysis. In summary, our study demonstrated that several factors are significantly related to patients with esophageal fistula and should be concerned about in clinical practice.
Insulin-Dependence Predicts Surgical Complications and Hospital Admission following Knee Arthroscopy
Authors: Traven Sophia A. , Reeves Russell A. , Walton Zeke J. , Woolf Shane K. , Slone Harris S. 
Published: January 2020

AbstractWhile prior studies have demonstrated that insulin-dependence is an independent risk factor for postoperative complications, morbidity, and mortality following spine and shoulder, hip, and knee arthroplasty, it has not been evaluated in the setting of knee arthroscopy. Therefore, the purpose of this study is to compare the risk of postoperative complications among patients with insulin-dependent diabetes mellitus and noninsulin-dependent diabetes mellitus (IDDM and NIDDM respectively) with the general population following knee arthroscopy. A retrospective analysis of the National Surgical Quality Improvement Program's database for the years 2005 to 2016 was conducted. Logistic regression analyses were used to assess the relationship between diabetic status and outcomes. Multivariate models were established to adjust for age, sex, body mass index, hypertension, congestive heart failure, chronic obstructive pulmonary disease, smoking status, American Society of Anesthesiology classification, and functional status. A total of 86,023 patients were identified. Patients with IDDM were at a much higher risk of surgical complications (odds ratio [OR]: 2.186, 95% confidence interval [CI]: 1.226–1.157), including deep infections (OR: 3.082, 95% CI: 1.753–5.419) and return to operating room [OR] (OR: 1.933, 95% CI: 1.280–2.919), as well as unplanned hospital admission (OR: 1.770, 95% CI: 1.289–2.431). However, NIDDM was not an independent risk factor for subsequent medical or surgical complications, unplanned hospital admission, or 30-day mortality. Patients with IDDM were much more likely to have surgical complications, including deep infection and return to OR, as well as unplanned hospital admission following knee arthroscopy. These risks diminished among those with NIDDM, with their adjusted risk profiles comparable to those without diabetes. Since diabetes occurs in a heterogenous state, more weight should be given to those with insulin-dependence when risk-stratifying patients for surgery. This is a Level III, retrospective comparison study.
Superconducting and pseudogap transition temperatures in high-Tc cuprates and the Tc dependence on pressure
Authors: Marino E C , Corrêa Reginaldo O , Arouca R , Nunes Lizardo H C M , Alves Van Sérgio 
Published: January 2020

Abstract
We derive universal analytic expressions for the critical temperatures of the superconducting (SC) and pseudogap (PG) transitions of the high-Tc
cuprates as a function of doping. These are in excellent agreement with the experimental data both for single-layered materials such as LSCO, Bi2201 and Hg1201 and multi-layered ones, such as Bi2212, Bi2223, Hg1212 and Hg1223. Optimal doping occurs when the chemical potential vanishes. We show that the SC coupling is enhanced with the number of layers, N, which allows for an accurate description of Tc in the Bi, Hg and Tl multi-layered families of cuprates. We also study the pressure dependence of the SC transition temperatures, obtaining excellent agreement with the experimental data for different materials and dopings. These results are obtained from an effective Hamiltonian for the itinerant oxygen holes, which includes both the electric repulsion between them and their magnetic interactions with the localized copper ions. We show that the former interaction is responsible for the PG and the latter, for the SC phases, the phase diagram of cuprates resulting from the competition between both. The Hamiltonian is defined on a bipartite oxygen lattice, which results from the fact that only the p

x
and p

y
oxygen orbitals alternatively hybridize with the 3d copper orbitals. From this, we can provide an unified explanation for the

d

x

2

−

y

2

symmetry of both the SC and PG order parameters and obtain the Fermi pockets observed in ARPES experiments.
Flux jumps in ring-shaped and assembled bulk superconductors during pulsed field magnetization
Authors: Zhou Difan , Shi Yunhua , Dennis Anthony R , Cardwell David A , Durrell John H 
Published: January 2020

Abstract
Bulk (RE)BCO, where RE is a rare-earth element or yttrium, superconductors fabricated in the form of rings are potentially useful for a variety of solenoidal-type applications, such as small, high field nuclear magnetic resonance and electromagnetic undulators. It is anticipated that the practical exploitation of these technologically important materials will involve pulse field magnetization (PFM) and, consequently, it is important to understand the behavior of ring-shaped samples subjected to the PFM process. Macroscopic flux jumps were observed in PFM experiments on ring-shaped bulk samples when the peak applied field reaches a threshold magnitude, similar to behavior reported previously in cylindrical samples. Magnetic flux jumps inward when the thermal instability is triggered, however it subsequently flows outwards from the sample, resulting in a relatively low trapped field. This behavior is attributed to a variety of effects, including the inhomogeneity of the material, which may lead to the formation of localized hot spots during the PFM process. In order to further elucidate this phenomena, the properties of a structure consisting of a bulk superconducting ring with a cylindrical superconductor core were studied. We observe that, although a flux jump occurs consistently in the ring, a critical state is established at the boundary of the ring-shaped sample and the core. We provide a detailed account of these experimental observations and provide an explanation in terms of the current understanding of the PFM process.
Piston crank mechanism simulation using finite element method
Authors: Miklos I Zs , Miklos C C , Alic C I 
Published: January 2020

Abstract
In this paper we present the analysis of the piston crank mechanism, using the finite element method using mechanical event simulation (MES). Mechanical event simulation is a modern process of modeling and analysis of mechanical systems, much different from the classical methods known in mechanical engineering. The use of MES involves a combination of kinematics of mechanical systems, dynamics of rigid bodies, respectively nonlinear stress analysis. So, mechanical event simulation is engineering by simulating a physical event in a virtual laboratory. The crank-piston mechanism analysis has been achieved with the Simulation Mechanical program, which aimed to determine the stress state, nodal displacement or relative elongations (strains) of mechanism elements, at different time steps of the kinematic cycle, depending on the kinematic and kinetostatic parameters variable according to different curves.
Control and monitoring of multi-disc comminution process
Authors: Kruszelnicka W , Tomporowski A , Flizikowski J , Kasner R 
Published: January 2020

Abstract
The article deals with the issues of intelligent, based on genetic algorithms, systems for grinding of grainy biomass to be used for further energy production processes. The aim of the study is to develop the basis for processing, active monitoring and subsequently, adaptive control of the process functional characteristics on the basis of solutions provided by artificial intelligence and identification of input variables of mathematical models and indexes, their mutual relations as well as their impact on the output variables such as: degree of fineness, production yield, power demand and unit energy consumption as well as CO2 emissions. A research methodology for practical control of the grinding potentials to improve the parameters of the multi disc grinding process parameters including: energy and power consumption, the product granulometry and the process efficiency improvement; CO2 emission index being a determinant of environmental efficiency has been developed. Parameters providing the grinding process with best possible conditions in terms of energy efficiency and emissivity, depending on the kind of the feed material, have been determined
Fuzzy decision system with a single variable parameter used in the intelligent control of the processes
Authors: Tirian G O , Chioncel C P , Holubeck R , Cinkar K 
Published: January 2020

Abstract
Based on the results obtained from the fuzzy systems presented in other published articles, a simplified fuzzy system was made, which was considered as the main factor the temperature difference. In this paper, another type of fuzzy system (single input and two outputs) has been developed and tested using the fuzzy toolbox in Matlab for viewing the resulting graphs and comparing them with the graphs resulting from the other types systems has come to the conclusion that the optimal solution is this fuzzy system with one input and two outputs.
Numerical research of thermal stress in a gas burner with a cylindrical mantle in relation to its construction
Authors: Prochaska B , Radić N , Gvero P 
Published: January 2020

Abstract
Over the last few years there has been a great interest in research into the improvement of gas condensing boilers with pre-mixing. Many researchers are concerned with the improvement of the construction of these boilers, whose individual parts directly affect some parameters such as combustion efficiency, burning intensity, flame stability and NOx emissions. The outer mantle of a cylindrical burner stands out among the parts of the structure as the said parameters depend on it This paper analyses the outer cylinder of the premixed burner in relation to thermal stress. Numerical FEM analysis was performed based on experimental measurements and CFD input data. The tests were carried out in different gas and different materials. The results obtained show different thermal stresses which are dependent on the different gas and materials on the outer cylindrical mantle. This indicates the necessity to optimize materials and patterns applied to the outer cylindrical mantle of the burner, which will be the subject of further research.
Study about wear of wheel’s bandage for C.F.R. 060 - EA 5100kW electric locomotive at circulation in curve with R = 300 m radius
Authors: Ursu-Neamţ G V 
Published: January 2020

Abstract
In this paper we study wear of lead wheels bandage for C.F.R. 060-EA 5100 kW at dynamic circulation in curve with 300 m radius. This study show how variation of wear factor is for lead wheels towards its admitted value on speed domain from 0 km/h until maximum speed of this locomotive in curve with articulated bogies. Among this it is also determined maximum admitted speed in curve impose by Technical Exploitation Regulation.
Influence of heating and moisture content on sludge drying process
Authors: Flori M , Miloştean D 
Published: January 2020

Abstract
Drying is one of the most efficient processes to reduce sludge volume by lowering moisture content. Vacuum filters are equipment suitable for sludge drying in small wastewater treatment plants. It is known that by reducing pressure in the filter, the vaporization process begins at lower temperature. The aim of this study is to estimate the values of liquid (free water) and gaseous (air) phase ratios in sludge cake during vacuum drying. Numerical results in function of heating temperature and cake initial moisture content are estimated by finite element analysis in a central point situated on cake surface.
Harnessing technology for mitigating water woes in the city of Bengaluru
Authors: Iyer L S , Giri S V 
Published: January 2020

Abstract
Industrialization has caused most of the world’s environmental problems like climate change, water security issues, biodiversity issues among others. Water-related issues like water scarcity, lack of water quality, water sanitation issues, lack of proper water resources management are some of them. Urbanization, population increase, pollution has led to an increase in water demand. Water being the elixir of life, is essential for the day-to-day living of an individual. The Fourth Industrial Revolution technologies like AI, IoT, Blockchain, Machine Learning have the capability of bringing solutions to these issues. The current study focuses on the water woes of Bengaluru, a fast-growing urban city, due to its migrating population. The woes are also due to the irresponsible behaviour of builders converting lakes into real estate infrastructure leading to clogged drains, excess sewage creation and flooding. A huge mismatch between demand and supply of water is created due to these issues. Before the city hits the Day Zero – no water day, it is significant to set up water infrastructure along with technology implementation which will help resolve this burning issue at the earliest.
Optimal Secret Text Compression Technique for Steganographic Encoding by Dynamic Ranking Algorithm
Authors: Jayapandiyan Jagan Raj , Kavitha C. , Sakthivel K. 
Published: January 2020

Abstract
In this research work, proposing an algorithm, which dynamically selects the best compression algorithms among several compression techniques for steganography encoding. Ranking and selection of best algorithm for each and every steganographic transaction is based on several factors like type of cover image being used for the transmission, length of the secret message, type of the message, compression ratio of the secret message being shared, encoding ratio of secret message over the medium etc., The proposed algorithm dynamically ranks and selects the right compression algorithm to be used for the given secret file to occupy lesser embedding space in stego-image.