bluuebunny/crossref_metadata_2025_split huggingface dataset:
Seroprevalence of human Brucellosis and associated risk factors among high-risk occupations in Mbeya Region of Tanzania
Authors: Sagamiko Frederick D. , Mfune Ruth L. , Hang’ombe Bernard M. , Karimuribo Esron D. , Mwanza Alfred M. , Sindato Calvin , Muma John B. 
Published: June 2020

Background: Brucellosis is a bacterial zoonotic disease of public health and economic importance. A cross-sectional study was conducted in Mbeya region between November 2015 and January 2016 to investigate the seroprevalence of human brucellosis and identify associated risk factors among individuals in risky occupations. Methods: A total of 425 humans from six occupational categories were serially tested for Brucella antibodies using the Rose Bengal Plate Test (RBPT) and competitive Enzyme Linked Immunosorbent Assay (c-ELISA), for screening and confirmation, respectively. A questionnaire survey was also administered to participants to collect epidemiological data. Results: The overall seroprevalence among the occupationally exposed individuals was 1.41% (95% CI: 00.64-3.12). Seroprevalence was higher among butcher men 5.6% (95% CI: 1.68-5.26), herds men 1.35% (95% CI: 0.18-9.02); and abattoir workers 1.1% (95% CI: 0.26-4.22) although there was no statistical significance. (P value = .18). Seroprevalence was also higher among men (1.8%) compared to females (0%) (P value = .19). and also, among those aged < 11 years (2.5%). Individuals who consumed raw milk had a higher seroprevalence (1.56%) compared to those who drunk boiled milk while seropositivity was 0.88% among those who assisted animals during parturition (P value = .49). Butcher men were at higher risk of exposure compared to other occupational categories. Our findings show the presence of brucellosis in occupationally exposed individuals in Mbeya region.Conclusion: There is need to sensitize the concerned professions in order to reduce the risk of acquiring Brucella infections from animals and animal products This also calls for public health awareness about the disease, and implementation of measures to prevent further spread of brucellosis within and outside the study area.
De waarheidsplicht en de geraden gevolgtrekking anno 2020: een zoektocht naar proportionaliteit
Authors: Seinen Cindy 
Published: June 2020

De waarheidsplicht en de geraden gevolgtrekking anno 2020: een zoektocht naar proportionaliteit
De waarheidsplicht komt in procedures steeds vaker aan de orde, ofwel omdat een partij om sanctionering ervan vraagt, dan wel omdat de rechter ambtshalve oordeelt dat sanctionering nodig is. Deze bijdrage behandelt de ontwikkelingen aan de hand van de typen gevolgtrekking die rechters sinds 2014 aan schendingen hebben verbonden.
Heel Holland spoort op
Authors: de Vries Arnout , Wemmers Shanna , Duijf Stan , Kallen Victor 
Published: June 2020

All citizens investigate crimes
Citizens investigating crimes themselves is a growing trend, because of democratization of information (e.g. social media), tools (e.g. apps) and knowledge (e.g. explanimations on YouTube). More and more citizens do their own research as modern Sherlocks. The police has to handle these trends in line with participant wishes and the law, but does not yet have concrete tools to do so. This article explores how the police participate in contemporary citizen criminal investigations, including the difficulties and benefits experienced. The obtained insights of the presented research serve as guidance, which can help police officers understand how to participate with citizens who have started, or want to start, a criminal investigation. The presented model explains how police can use it to better guide and stimulate, but also stop or protect citizens in their investigative activities. An app with professional guidance was piloted in four police units to participate with citizens that do their own research and learn from expectations and experiences. Citizens need guidance, but more importantly expect a certain degree of reciprocity in collaborating with police in criminal investigations.
Contribuições da concepção de aprendizagem significativa de David Ausubel na formação de jovens aprendizes.
Authors: Oliveira Marcelo Silva , Carvalho Marco Antônio de , Mariano Sangelita Miranda Franco 
Published: June 2020

O presente artigo tem como propósito refletir sobre as contribuições da concepção de Aprendizagem Significativa de David Ausubel (1982) para a formação de aprendizes. Objetiva analisar a constituição do processo de ensino e aprendizagem do Programa Jovem Aprendiz (PJA) a partir de práticas educativas influenciadas pelas múltiplas contradições que permeiam a ação educacional demarcada por um espaço-tempo histórico, social e cultural. Este estudo bibliográfico estrutura-se a partir de: apontamentos iniciais sobre o PJA, aprendizagem e metodologia utilizada; discussão sobre aprendizagem significativa e prática educativa; caracterização do PJA e suas diretrizes bem como a interlocução entre estas e alternativas pedagógicas formativas. Conclui-se que a aprendizagem significativa resulta de uma prática pedagógica, que auxilia a formação da jovem classe trabalhadora, pois alicerçada ao conhecimento prévio, na análise crítica e intencionalidades garante o sucesso das ações realizadas, impedindo que o ensino fique restrito à esfera da idealização e desconectado de sua objetivação.
Pedagogias em diferentes espaços educativos
Authors: Terçariol Adriana Aparecida de Lima , Teixeira Rosiley Aparecida 
Published: June 2020

A publicação do número 34 da Revista Dialogia oportuniza o compartilhamento de estudos inéditos decorrentes de pesquisas e experiências desencadeadas em diferentes espaços educacionais que evidenciam concepções, princípios, encaminhamentos metodológicos, avanços, desafios, entre outros aspectos. Com isso, a comunidade científica é brindada com pesquisas que retratam as possibilidades pedagógicas para diferentes contextos e sujeitos, reconhecendo suas especificidades, propiciando assim reflexões entre Educação e práxis social.Desejamos uma boa leitura!Adriana Aparecida de Lima TerçariolRosiley Aparecida Teixeira(Editoras)
Manipulating Complex Robot Behavior for Autonomous and Continuous Operations
Authors: Liu Chengliang , Gong Liang , Zhang Wei 
Published: June 2020

Service robot control faces challenges of dynamic environment and complex behavior, which mainly include eye-hand coordination and continuous operations. However, current programming scheme lacks the ability of managing such tasks. In this chapter, we propose a methodology of software development paradigm for the continuous operation of the dual-arm picking robot. First, a dual-arm robot is built for picking with the purpose of selectively harvesting in plant factory. Second, a hierarchical control software is framed by means of “Sense Plan Act” (SPA) paradigm. Third, based on the previous design, programming concept, and the ROS system, the sub-node programming of visual module, motion module, eye-hand coordination module, and task planning module are implemented with a state machine-based architecture. The experimental results show that if total number of targets within the visual field is not more than three, the average picking time is less than 35 s. The fluency of concurrent task management shows the feasibility of manipulating complex robot behavior for autonomous and continuous operations with the finite state machine model and task level architecture.
Plum Breeding
Authors: Butac Madalina 
Published: June 2020

Worldwide, plum is one of the main species, occupying an area of about 2,600,000 ha and ensuring production about 11,700,000 tons. Even though there are over 6000 plum cultivars belonging to 19–40 species, there is still the need to create new cultivars due to the demands of growers and consumers. In addition, the large plum-growing countries (Romania, Serbia, Germany, Bulgaria, etc.) have decreased production due to plum pox virus (PPV) attack. Therefore, these countries developed breeding programs with the following objectives: resistance/tolerance to PPV, productivity, fruit quality, late blooming, self-fertility, different ripening times, short growing period, spur fructification, etc. Using different breeding methods (controlled hybridization, open pollination, selection in wild population on Prunus sp., and mutagenesis), in the last years, over 450 plum cultivars were released, from which 70% represent European cultivars and 30% Japanese cultivars.
Beyond the Quality of Life in Bowel Dysfunction after Spinal Cord Injury: Approaches to the Consequences in Motility, Immune System, and Microbiome
Authors: de la Cruz-Castillo Estefanía , García-Vences Elisa 
Published: June 2020

Spinal cord injury (SCI) is a harmful event that involves several repercussions on sensory and motor function that affects the quality of life (QoL) of patients. After SCI, many damage mechanisms are activated that impact on both autonomous extrinsic and intrinsic innervation toward the gut, and these changes modify the gut motility causing bowel dysfunction (BD), an entity that affects 40% of patients with SCI, being the second comorbidity after loss of mobility with no recognized cure. The severity of complications is ruled by the level and severity of injury, having a worse prognosis with an injury that is the most proximal to the brain. In the last 5 years, some experiments have tried to elucidate the consequences of dysbiosis in the gut and aggregated proinflammatory processes. The goal of this chapter is to establish the importance of bacterial composition and immune system repercussions in bowel dysfunction after SCI and how could it give rise to new therapies.
Clonal diversity and genetic variation of the sedge Carex nigra in an alpine fen depend on soil nutrients
Authors: Reisch Christoph , Meier Stefanie , Schmid Christoph , Bartelheimer Maik 
Published: June 2020

In this study we analysed the impact of water regime and soil nutrients on the clonal diversity and genetic variation of the sedge Carex nigra in a central alpine fen. For our analysis, we established 16 study plots randomly distributed over the fen. We determined the exact elevation of each plot as an indicator for the water regime and measured the content of phosphorous and potassium in the soil of each plot. Clonal diversity and genetic variation of C. nigra were assessed with nuclear microsatellites using leaf material collected in 20 subplots along a diagonal cross within each study plot. The influence of water regime and soil mineral nutrients on clonal diversity and genetic variation was estimated by Bayesian multiple regression. Our study revealed a clear impact of soil nutrient conditions on clonal diversity and genetic variation of C. nigra, which increased with the concentration of phosphorous and decreased with the concentration of potassium. Key background to these findings seems to be the relative offspring success from generative as compared to clonal propagation. Phosphorous acquisition is essential during seedling establishment. Clonal diversity and genetic variation increase, therefore, at sites with higher phosphorous contents due to more successful recruitment. High levels of clonal diversity and genetic variation at sites of low potassium availability may in contrast be mainly caused by increased plant susceptibility to abiotic stress under conditions of potassium deficiency, which brings about more gaps in C. nigra stands and favors the ingrowth from other clones or recruitment from seeds.
Simultaneous determination of phenolic metabolites in Chinese citrus and grape cultivars
Authors: Chen Yuan , Hong Yanyun , Yang Daofu , He Zhigang , Lin Xiaozi , Wang Guojun , Yu Wenquan 
Published: June 2020

Background
As the major bioactive compounds in citrus and grape, it is significant to use the contents of flavonoids and phenolic acids as quality evaluation criteria to provide a better view of classifying the quality and understanding the potential health benefits of each fruit variety.

Methods
A total of 15 varieties of citrus and 12 varieties of grapes were collected from Fujian, China. High-performance liquid chromatography method was used for the simultaneous determination of 17 phenolic compounds, including gallic acid, chlorogenic acid, caffeic acid, syringic acid, ρ-coumaric acid, ferulic acid, benzoic acid, salicylic acid, catechin, epicatechin, resveratrol, rutin, naringin, hesperidin, quercetin, nobiletin and tangeritin in the peels of citrus and grape cultivars. Further, the cultivars of citrus and grape were classified using principal component analysis (PCA) and hierarchical cluster analysis (HCA).

Results
A thorough separation of the 17 compounds was achieved within 100 min. The tested method exhibited good linearity (the limits of detection and limits of quantification were in the range of 0.03–1.83 µg/mL and 0.09–5.55 µg/mL, respectively), precision (the relative standard deviations of repeatability were 1.02–1.97%), and recovery (92.2–102.82%) for all the compounds, which could be used for the simultaneous determination of phenolic compounds in citrus and grape. Hesperidin (12.93–26,160.98 µg/g DW) and salicylic acid (5.35–751.02 µg/g DW) were the main flavonoids and phenolic acids in 15 citrus varieties, respectively. Besides, the hesperidin (ND to 605.48 µg/g DW) and salicylic acid (ND to 1,461.79 µg/g DW) were found as the highest flavonoid and the most abundant phenolic acid in grapes, respectively. A total of 15 citrus and 12 grape samples were classified into two main groups by PCA and HCA with strong consistency.
Identification of gene expression and DNA methylation of SERPINA5 and TIMP1 as novel prognostic markers in lower-grade gliomas
Authors: Zeng Wen-Jing , Yang Yong-Long , Wen Zhi-Peng , Chen Peng , Chen Xiao-Ping , Gong Zhi-Cheng 
Published: June 2020

Background
Lower-grade gliomas (LGGs) is characteristic with great difference in prognosis. Due to limited prognostic biomarkers, it is urgent to identify more molecular markers to provide a more objective and accurate tumor classification system for LGGs.

Methods
In the current study, we performed an integrated analysis of gene expression data and genome-wide methylation data to determine novel prognostic genes and methylation sites in LGGs.

Results
To determine genes that differentially expressed between 44 short-term survivors (<2 years) and 48 long-term survivors (≥2 years), we searched LGGs TCGA RNA-seq dataset and identified 106 differentially expressed genes. SERPINA5 and TIMP1 were selected for further study. Kaplan–Meier plots showed that SERPINA5 and TIMP1 expression were significantly correlated with overall survival (OS) and relapse-free survival (RFS) in TCGA LGGs patients. We next validated the correlation between the candidate genes expression and clinical outcome in CGGA LGGs patients. Multivariate analysis showed that TIMP1 mRNA expression had a significant prognostic value independent of other variables (HR = 4.825, 95% CI = 1.370–17.000, P = 0.014). Then, differential methylation sites were identified from differentially candidate gene expression groups, and all four methylation sites were significantly negatively correlated with gene expression (spearman r < − 0.5, P < 0.0001). Moreover, hyper-methylation of four methylation sites indicated better OS (P < 0.05), and three of them also shown statistical significantly association with better RFS, except for SERPINA5 cg15509705 (P = 0.0762).

Conclusion
Taken together, these findings indicated that the gene expression and methylation of SERPINA5 and TIMP1 may serve as prognostic predictors in LGGs and may help to precise the current histology-based tumors classification system and to provide better stratification for future clinical trials.
The effect of journal guidelines on the reporting of antibody validation
Authors: Hoek Joyce M. , Hepkema Wytske M. , Halffman Willem 
Published: June 2020

Background
Despite the widespread use of antibodies as a research tool, problems with specificity, lot-to-lot consistency and sensitivity commonly occur and may be important contributing factors to the ‘replication crisis’ in biomedical research. This makes the validation of antibodies and accurate reporting of this validation in the scientific literature extremely important. Therefore, some journals now require authors to comply with antibody reporting guidelines.

Methods
We used a quasi-experimental approach to assess the effectiveness of such journal guidelines in improving antibody reporting in the scientific literature. In a sample of 120 publications, we compared the reporting of antibody validation and identification information in two journals with guidelines (Nature and the Journal of Comparative Neurology) with two journals without guidelines (Science and Neuroscience), before and after the introduction of these guidelines.

Results
Our results suggest that the implementation of antibody reporting guidelines might have some influence on the reporting of antibody validation information. The percentage of validated antibodies per article slightly increased from 39% to 57% in journals with guidelines, whereas this percentage decreased from 23% to 14% in journals without guidelines. Furthermore, the reporting of validation information of all primary antibodies increased by 23 percentage points in the journals with guidelines (OR = 2.80, 95% CI = 0.96-INF; adjusted p = 1, one-tailed), compared to a decrease of 13 percentage points in journals without guidelines. Fortunately, the guidelines seem to be more effective in improving the reporting of antibody identification information. The reporting of identification information of all primary antibodies used in a study increased by 58 percentage points (OR = 17.8, 95% CI = 4.8-INF; adjusted p = 0.0003, one-tailed) in journals with guidelines. This percentage also slightly increased in journals without guidelines (by 18 percentage points), suggesting an overall increased awareness of the importance of antibody identifiability. Moreover, this suggests that reporting guidelines mostly have an influence on the reporting of information that is relatively easy to provide. A small increase in the reporting of validation by referencing the scientific literature or the manufacturer’s data also indicates this.

Conclusion
Combined with the results of previous studies on journal guidelines, our study suggests that the effect of journal antibody guidelines on validation practices by themselves may be limited, since they mostly seem to improve antibody identification instead of actual experimental validation. These guidelines, therefore, may require additional measures to ensure effective implementation. However, due to the explorative nature of our study and our small sample size, we must remain cautious towards other factors that might have played a role in the observed change in antibody reporting behaviour.
Forecast and Analyze the Revenue of Biman Bangladesh Airlines Limited Based on ARIMA Model
Authors: Rahman Sumaiya , Ahmed Shohel , Faruq Tahrima 
Published: June 2020

Background: As a public limited company in Bangladesh, Biman Bangladesh Airlines Limited has been struggling to establish itself as a profitable company after taking many initiatives. The work presented in this article constitutes a contribution to modeling and forecasting the financial positionof Biman Bangladesh by using a time series approach.Methodology: The article demonstrates how the income and expenditure data could be utilized to forecast future profit scenarios by developing several Autoregressive Integrated Moving Average (ARIMA) time series with the regression model. Utilizing the Akaike Information Criterion (AIC) values, we identify the best fit ARIMA model and use this to forecast the financial scenarios for the subsequent years. To successfully build the model we use R Programming.
Results and Conclusion: The model predicts future values of income, expenditure and using these two, the profit or loss scenarios can be used for forecasting from year 2018 to 2025. The results forecast that Income would increase or decrease in contest of the Expenditure. As a result Biman Bangladesh may have face significant losses in the years 2020, 2021 and 2024.
Food Consumption Practices of Men and Women across Rural-Urban Interface of South Indian Megacity Bangalore
Authors: Geetha K. , Yatnatti Shilpa , Vijayalakshmi D. , Dittrich Christoph 
Published: June 2020

Background: Food consumption practices involving dietary diversity, healthy and unhealthy practices have greater influence on nutritional and health status of the individual. Men and women always behave differently and have different consumption pattern due to various factors. Urbanization gradients along rural-urban interface of Bangalore mega city helps for comparative study of these factors.
Aims: To compare food consumption practices between men and women across rural-urban interface of Bangalore, India.
Methodology: Men (n=150) and women (n=150) from 300 middle income households in the rural-urban interface of Bangalore, were surveyed for dietary diversity score (DDS), healthy and unhealthy dietary practices and response to questions on health and nutrition.
Results: Findings revealed that, least DDS was recorded in transition area among both men (48.0%) and women (47.7%). In rural maximum difference for healthy habit score was existed between men (50.8%) and women (44.0%). Average unhealthy habits score was more among women in rural (33.2%) and transition (35.4%) areas, whereas in urban, men had higher score (41.8%). Health and nutrition aspects indicated, fasting on religious belief was more practiced by women in transition area (56%). Consumption of health supplements was more among women, especially in urban (34%).
Conclusion: It can be concluded that, women have poor food consumption practices compared to men. Even though women are observed to be more health conscious than men, their dietary habits are compounded with various factors such as socio-cultural, occupational and urbanization. In this regard nutrition programmes must be strengthened to decrease risk factors for non-communicable diseases and to improve overall health of the individuals.
Characterization, Manipulation, and Isolation ofParamecium aureliaUsing a Micro‐Electromigration Chip and Computer Vision
Authors: Shunmugam Ashaa Preyadharishini , Fernandez Javier G. 
Published: June 2020

AbstractManipulating microorganisms with inherent motility is a challenging yet significant aim with implications in many biological, environmental, and technological applications. Many microorganisms that are broadly available in nature can be used as self‐powered systems that can be directed with external stimuli.Parameciumis a unicellular protozoan that exhibits a negative galvanotaxis where the cell follows the direction of weak electric fields. Here, the galvanotactic behavior ofParameciais studied to achieve the precise manipulation of these organisms. Using a specially devised microfluidic chip and computer vision, unprecedented levels of manipulation and isolation ofParameciaare demonstrated, enabling their integration, use, and study in micro‐electromechanical systems.