bluuebunny/crossref_metadata_2025_split huggingface dataset:
The RAS‐PI3K‐AKT‐NF‐κB pathway transcriptionally regulates the expression of BCL2 family and IAP family genes and inhibits apoptosis in fibrous epulis
Authors: Jiang Yangyang , Fang Bing , Xu Bin , Chen Liang 
Published: November 2019

AbstractBackgroundEpulis has a tumor‐like appearance but is considered to be a massive reactive lesion rather than a true neoplasia. Limited information about the pathogenesis of epulis is available. The purpose of our study was to identify potential signaling pathways in fibrous epulis through transcriptome profiling.MethodsDifferentially expressed genes (DEGs) between fibrous epulis lesions and normal gingival tissues were detected using RNA sequencing (RNAseq). The expression levels of eighteen genes were validated using quantitative real‐time PCR (qRT‐PCR).ResultsRNAseq identified 533 upregulated genes and 732 downregulated genes. The top 10 upregulated genes were IL11, OSM, MMP3, KRT75, MMP1, IL6, IL1B, IL24, SP7, and ADGRG3. The top 10 downregulated genes were BCHE, TYR, DCT, KRT222, RP11‐507K12.1, COL6A5, PMP2, GFRA1, SCN7A, and CDH19. KEGG pathway analysis further indicated that the DEGs were enriched in “Pathways in cancer” and the “Ras signaling pathway”. quantitative real‐time PCR verified that the expression levels of SOS1, HRAS, PIK3CA, AKT3, IKBKA, IKBKB, NFKB1, BCL2, BCL2L1, XIAP, BIRC2, and BIRC3 were increased significantly.ConclusionsThe current transcriptomic profiling study reveals that in fibrous epulis, RAS‐PI3K‐AKT‐NF‐κB pathway transcriptionally regulates the expression of BCL2 family and IAP family genes, leading to increased proliferation and apoptosis inhibition.
Preparation of TiO2 supported on MDF biochar for simultaneous removal of methylene blue by adsorption and photocatalysis
Authors: Silvestri Siara , Stefanello Nádia , Sulkovski Aline Alexia , Foletto Edson Luiz 
Published: November 2019

AbstractBACKGROUNDThis article reports for the first time the preparation and characterization of biochar/titanium dioxide (TiO2) composites by pyrolysis from medium‐density fiberboard (MDF) biomass. Their adsorptive and photocatalytic activities were evaluated by removal of methylene blue (MB) dye from aqueous solutions with and without UV‐light irradiation.RESULTSCharacterization results suggested that the composite have different intrinsic characteristics in relation to the precursors. The biochar and TiO2 amounts of the composite significantly affected its performance. The MB removal efficiency with 1 g L−1 of the composite with 10% w/w TiO2 (B10T sample) was 2.3 and 2.2 times faster than that the B5T and B1T samples, respectively, and 1.5 times faster than TiO2 alone.CONCLUSIONThe loading of TiO2 significantly enhanced the adsorption performance of the composite and factors such as specific surface area, pore volume and pore size of the biochar synergistically promoted the photocatalytic activity of TiO2, coupled with a lower band gap for all composites produced. Both adsorption and photocatalytic efficiencies were confirmed to contribute to the degradation of the aqueous solution of MB by the hydroxyl radical (•OH) in lower molecular mass by‐products. The synthesized composite is a promising alternative sustainable material for removing chemical contaminants, since the biochar used as a support comes from the waste of the furniture industry. © 2019 Society of Chemical Industry
Controlling the l‐asparaginase extraction and purification by the appropriate selection of polymer/salt‐based aqueous biphasic systems
Authors: Magri Agnes , Pimenta Marcela V , Santos João HPM , Coutinho João AP , Ventura Sónia PM , Monteiro Gisele , Rangel‐Yagui Carlota O , Pereira Jorge FB 
Published: November 2019

AbstractBACKGROUNDl‐Asparaginase (ASNase) is an important biopharmaceutical for the treatment of acute lymphoblastic leukemia (ALL); however, with some restrictions due to its high manufacturing costs. Aqueous biphasic systems (ABS) have been suggested as more economical platforms for the separation/purification of proteins, but a full understanding of the mechanisms behind the ASNase partition is still a major challenge. Polymer/salt‐based ABS with different driving‐forces (salting‐out and hydrophilicity/hydrophobicity effects) were herein applied to control the partition of commercial ASNase.RESULTSThe main results showed the ASNase partition to the salt‐ or polymer‐rich phase depending on the ABS studied, with extraction efficiencies higher than 95%. For systems composed of inorganic salts, the ASNase partition was controlled by the polyethylene glycol (PEG) molecular weight used. Cholinium‐salts‐based ABS were able to promote a preferential ASNase partition to the polymer‐rich phase using PEG‐600 and to the salt‐rich phase using a more hydrophobic polypropylene glycol (PPG)‐400 polymer. It was possible to select the ABS composed of PEG‐2000 + potassium phosphate buffer as the most efficient to separate the ASNase from the main contaminant proteins (purification factor = 2.4 ± 0.2), while it was able to maintain the enzyme activity for posterior application as part of a therapeutic.CONCLUSIONPolymer/salt ABS can be used to control the partition of ASNase and adjust its purification yields, demonstrating the ABS potential as more economic platform for the selective recovery of therapeutic enzymes from complex broths. © 2019 Society of Chemical Industry
Biosensing of algal‐photosynthetic productivity using nanostructured bioelectrochemical systems
Authors: Mahmoud Rehab H , Abdo Sayeda M , Samhan Farag A , Ibrahim Mohamed K , Ali Gamila H , Hassan Rabeay YA 
Published: November 2019

AbstractBACKGROUNDMicroalgae have attracted worldwide interest resulting from their extensive applications in renewable energy and biomass production. However, in algal fuel cells, photosynthetically evolved oxygen hinders the use of algal biofilms formed at the anode surface. Here, nanostructured bio‐electrochemical systems have been designed to explore the algal bio‐electrochemistry at different illumination and growth conditions.RESULTSThree algal strains were screened for their exoelectrogenic activity and the possibility of direct electron transfer to the chemically modified surfaces. After adjusting light and dark conditions and medium compositions, oxygen production within the fuel cells was regulated. At the anode surface, the obtained bioelectrochemical responses and the morphological characterizations suggested that Oscillatoria agardhii has the potential to serve as electron donor and the nanostructured surface is the final electron acceptor. To that end, dual‐chamber algal fuel cells were constructed and glucose (10 g L–1) was used as carbon source.CONCLUSIONPower density of 26.8 mW m–2 was produced using the biofilm formed by O. agardhii. Eventually, the consumption of organic waste was monitored, whereas the chemical oxygen demand removal reached 82%. © 2019 Society of Chemical Industry
Organic phase screening for in‐stream reactive extraction of bio‐based 3‐hydroxypropionic acid: biocompatibility and extraction performances
Authors: Sánchez‐Castañeda Ana Karen , Moussa Marwen , Ngansop Luther , Trelea Ioan Cristian , Athès Violaine 
Published: November 2019

AbstractBACKGROUND3‐Hydroxypropionic acid (3‐HP) production through glycerol bioconversion by Lactobacillus reuteri suffers from low yields and productivities due to product inhibition. Reactive extraction assisted by a Hollow Fibre Membrane Contactor (HFMC) is a promising strategy for process intensification. However, the use of this integrated system is hindered by extraction phase toxicity towards the microorganism. This study describes a solvent selection strategy based on extraction performance (extraction yield and viscosity, related to mass transfer), and a low solvent toxicity, in order to find an extraction phase composition that allows continuous in stream extraction of 3‐HP.RESULTSInert diluent addition to a trioctylamine (TOA)‐decanol mixture decreased its toxicity and viscosity but decreased extraction yield. The linear and ramified long‐chain alcohols tested showed that increasing the number of carbon atoms decreased extraction performance as well as toxicity. Ramified alcohols showed the lowest extraction performance. Didodecylmethylamine (DDMA) gave higher extraction yield and lower solvent toxicity than TOA. Flow cytometry with dual staining for cell membrane integrity and enzymatic activity proved to give concordant and complementary information with cell bioconversion ability, being an adequate and quick method for solvent toxicity assessment. The selected organic phase consisted of 20% DDMA, 47% dodecanol and 33% dodecane by volume, and can be used for in‐stream extraction of 3‐HP produced by L. reuteri.CONCLUSIONSThe integrated selection criteria proposed in this study – extraction yield, solvent viscosity and toxicity – provide key information for choosing an organic phase with the best trade‐off between extraction performance and biocompatibility. © 2019 Society of Chemical Industry
Genome‐wide identification of nonvisual opsin family reveals amplification of RPE‐retinal G protein receptor gene (RGR) and offers novel insights into functions of RGR(s) in Paralichthys olivaceus (Paralichthyidae, Teleostei)
Authors: Liu Yuezhong , Zhang Wei , Du Xinxin , Liu Yuxiang , Qu Jiangbo , Liu Xiaobing , Liu Jinxiang , Zhang Quanqi 
Published: November 2019

AbstractOpsins play important roles in the image‐forming visual pathways and numerous biological systems such as the biological clock and circadian rhythm. However, the nonvisual opsins involved in nonimage forming process are not clear to date. The aim of this study was to characterize nonvisual opsins in Paralichthys olivaceus. A total of 24 nonvisual opsin genes were identified. Expressions of these genes in eye, brain, heart, testis, and fin were investigated by quantitative real‐time polymerase chain reaction (qRT‐PCR). Testis contained a surprisingly large number of nonvisual opsins including Opn4m2, Tmt2a, Tmt3b, Opn3, RRH, Opn7a, and Opn7b. Syntenic and phylogenetic analyses confirmed that the RGRa and RGRb originated from the teleost‐specific genome duplication (TSGD). qRT‐PCR results demonstrated high RGRa and RGRb expression in the eye, while the expression levels in the brain, heart, testis, and fin were relatively weak. In situ hybridization results presented here revealed the presence of both RGRa and RGRb mRNA‐positive signals in the ganglion cell layer but absence in the intracellular compartment of retinal pigment epithelium (RPE) and Müller glial cells. Therefore, we hypothesized that RGRa and RGRb had undergone subfunctionalization in P. olivaceus after TSGD. In conclusion, this study provides novel insights into the evolutionary fates of the RGR genes, still, further studies need to be done to explore the mechanism about the lack of RGR genes' expression in RPE.
Three‐component, one‐pot synthesis of pyrano[3,2‐c]chromene derivatives catalyzed by ammonium acetate: Synthesis, characterization, cation binding, and biological determination
Authors: Mhiri Chiheb , Boubakri Lamia , Ternane Riadh , Mansour Lamjed , Harrath Abdel Halim , Al‐Tamimi J. , Baklouti Lassaad , Hamdi Naceur 
Published: November 2019

AbstractThree‐component reaction of arylaldehydes with malononitrile and 4‐hydroxycoumarine using CH3COONH4 as a catalyst at reflux was used for the synthesis of novel substituted pyrano[3,2‐c]chromene derivatives. The structure of these compounds was assigned by spectroscopic data such as (IR, 1HNMR, 13CNMR, and mass spectral data). The cation binding properties of chromene derivatives 4a‐c towards Cu2+, Ni2+, and Zn2+ were studied in methanol. The results showed that Zn2+ is the most complexed in this series of cations, and 4c is best complexed with either Ni2+ and Zn2+. Antimicrobial properties of new pyrano[3,2‐c]chromene derivatives are investigated, the compound 4c presents against Micrococcus luteus LB 14110 an MIC value of 0.0185 mg/mL quite better to that of ampicillin (0.0195 mg/mL) used as standard. Concerning acetylcholinesterase inhibition activity (AChEI), compound 4c presents an interesting AChEI activity with an inhibition of 52%.
Evolution of annual report: A longitudinal investigation between 1973 and 2015
Authors: Uyar Ali 
Published: November 2019

AbstractThis study analyzes how corporate annual reports have evolved between 1973 and 2015. The coverage of annual reports is cyclical while the average cycle spans over several years rather than one single year. We suppose that external shocks such as the financial crisis affect this trend. The results show that reports have substantially improved in content and design and presented more information that is relevant for the stakeholders. In particular, annual reports have evolved into more comprehensive business documentation. It is undergoing an evolution in financial information as well as in nonfinancial data. Although the stockholders were the main target of annual reports in earlier years, the stakeholders seem to be the main target of the report in later years. Over time, reports gained more professional look while presenting more voluntary and mandatory information.
Dietary supplementation with pioglitazone hydrochloride and chromium methionine manipulates lipid metabolism with related genes to improve the intramuscular fat and fatty acid profile of yellow‐feathered chickens
Authors: Jin Cheng‐long , Zeng Huan‐ren , Gao Chun‐qi , Yan Hui‐chao , Tan Hui‐ze , Wang Xiu‐qi 
Published: November 2019

AbstractBACKGROUNDIntramuscular fat (IMF) and polyunsaturated fatty acids (PUFAs) have been thought to play a crucial role in improving meat quality. Considering the ability of pioglitazone hydrochloride (PGZ) to deposit fat, and the anti‐stress capability of chromium methionine (CrMet), we combined these compounds to produce higher quality meat in poultry. A total of 3000 female chickens were divided into four groups (five replicates, each with 150 chickens): control, control plus15 mg·kg−1 PGZ, control plus 200 μg·kg−1 CrMet, and control plus15 mg·kg−1 PGZ plus 200 μg·kg−1 CrMet. The experiment lasted for 28 days.RESULTSCompared to the control group and the PGZ group, the average daily gain (ADG) was significantly increased in the PGZ plus CrMet group, whereas the feed‐to‐gain ratio (F/G) was decreased from 0 to 14 days. Meanwhile, the redness value of breast muscle and IMF of thigh muscle increased in the PGZ plus CrMet group compared with the control group and these detections in the PGZ plus CrMet group exhibited highest value among the four groups. The cooking loss decreased in the breast muscle and thigh muscle after PGZ combined with CrMet in diets. The percentages of C16:1, C18:2n‐6 and PUFAs increased in the PGZ plus CrMet group. The mRNA abundance of peroxisome proliferator activated receptor (PPAR) γ, PPAR coactivator 1 α, and fatty acid binding protein 3 was significantly enhanced with PGZ plus CrMet supplementation.CONCLUSIONCollectively, dietary supplementation with PGZ plus CrMet improved growth performance and meat quality by decreasing the cooking loss and increasing the IMF and PUFA levels. © 2019 Society of Chemical Industry
Functional and conformational properties of proteolytic enzyme‐modified potato protein isolate
Authors: Akbari Nastaran , Mohammadzadeh Milani Jafar , Biparva Pourya 
Published: November 2019

AbstractBACKGROUNDPotato protein hydrolysates (PPHs) were preparedwith Alcalase on intact potato protein isolates (PPI), with differenthydrolysis times (0.5–4 h), and functional and conformational properties of resultant hydrolysates were investigated.RESULTSThe degree of hydrolysis changed during incubation. Peptide bond cleavage increased and hydrolysis progressed rapidly. Gel electrophoresis showed that, by increasing the hydrolysis time, peptides with an apparent molecular weight below 20 kDa increased. It also revealed that, among potato protein components, patatin was more sensitive to Alcalase® hydrolysis than protease inhibitors. Enzymatic hydrolysis significantly enhanced the solubility and foam capacity of PPHs, but impaired foam stability (P < 0.05). Limited enzymatic hydrolysates (0.5PPH) at the interface improved the emulsion activity and stability index. These emulsions also had the smallest z‐average and polydispersity index and showed the highest zeta potential. Fourier‐transform infrared spectrometry (FTIR) analysis indicated extensive disruption of hydrogen bonds in PPHs, besides augmentation of α‐helices and β‐turns, and a decline in the β‐sheets in the secondary structure of the PPHs was shown.CONCLUSIONPotato protein isolate, especially 0.5PPH, has good functional and conformational properties. Overall, our results provide new insights into the use of potato protein hydrolysates as a functional food component in the food industry. © 2019 Society of Chemical Industry
Feasibility of Resistance Exercise for Posttraumatic Stress and Anxiety Symptoms: A Randomized Controlled Pilot Study
Authors: Whitworth James W. , Nosrat Sanaz , SantaBarbara Nicholas J. , Ciccolo Joseph T. 
Published: November 2019

AbstractEmerging evidence suggests that exercise may beneficially affect posttraumatic stress symptoms (PTSS), but few randomized trials exist. Additionally, the effects of resistance exercise (i.e., weight lifting or strength training) on PTSS have not been thoroughly examined. This study aimed to explore the feasibility of a brief high‐intensity resistance exercise intervention for PTSS and related issues, such as anxiety, sleep, alcohol use, and depression, in non‐treatment–seeking adults who screened positive for posttraumatic stress disorder (PTSD) and anxiety. The sample included 30 non‐treatment–seeking, urban‐dwelling adults (M age = 29.10 years, SD = 7.38; 73.3% female) who screened positive for PTSD and anxiety and were randomly assigned to either a 3‐week resistance exercise intervention or a time‐matched contact control condition. The results suggest the intervention was feasible, with 80.0% (n = 24) of participants completing the study, 88.9% of the resistance exercise sessions attended, and no adverse effects reported. Additionally, resistance exercise had large beneficial effects on symptoms of avoidance, d = 1.26, 95% CI [0.39, 2.14]; and hyperarousal, d = 0.90, 95% CI [0.06, 1.74], relative to the control condition. Resistance exercise also produced large improvements concerning sleep quality, d = 1.31, 95% CI [0.41, 2.21], and hazardous alcohol use, d = 0.99, 95% CI [0.13, 1.86], compared to the control condition. Overall, the findings suggest that 3 weeks of high‐intensity resistance exercise is a feasible intervention for PTSS reduction in non‐treatment–seeking adults who screen positive for PTSD and anxiety; additional research is needed to verify these preliminary findings.
Dogs and Disease Threats to Giant Pandas in China
Authors: Yan Xia , Owens Jacob R. , Wen Yiping , Su Xiaoyan , Wang Zhenghao , Liu Songrui , Zhang Dongsheng , Callan Ramana , Wenlei Bi , Qi Dunwu , Spotila James R. , Hou Rong , Zhang Zhihe 
Published: November 2019

ABSTRACTThe potential threat of domestic dogs to wildlife habitat in China is not widely recognized, despite their large population, lack of regulations regarding their control, and threat they pose to native species. In a case study in 2017, we surveyed villages surrounding Liziping Nature Reserve, the primary site for the release of captive‐born giant pandas (Ailuropoda melanoleuca) into the wild. We conducted surveys of dog owners to assess the population size, demographics, free‐roaming status, and vaccination and sterilization history of their dogs. We collected blood and fecal samples to assess the prevalence of viral and parasite disease threats. At least 370 owned dogs lived near the core giant panda habitat; 64% were free‐roaming, 21% had positive antibody titers for ≥1 of the 4 viruses we tested (canine distemper, parvovirus, rotavirus, rabies), and 67% were positive for gastrointestinal parasites. The high proportion of free‐roaming dogs, uninhibited access to the reserve, and high prevalence of infectious diseases indicate that dogs pose a serious threat to wildlife within Liziping. The extent of this threat throughout the giant panda nature reserve network is unknown and should be assessed. © 2019 The Authors. Journal of Wildlife Management published by Wiley Periodicals, Inc. on behalf of The Wildlife Society.
The Sustainability of Wolverine Trapping Mortality in Southern Canada
Authors: Mowat Garth , Clevenger Anthony P. , Kortello Andrea D. , Hausleitner Doris , Barrueto Mirjam , Smit Laura , Lamb Clayton , DorsEy BenJAMIN , Ott Peter K. 
Published: November 2019

ABSTRACTRange declines, habitat connectivity, and trapping have created conservation concern for wolverines throughout their range in North America. Previous researchers used population models and observed estimates of survival and reproduction to infer that current trapping rates limit population growth, except perhaps in the far north where trapping rates are lower. Assessing the sustainability of trapping requires demographic and abundance data that are expensive to acquire and are therefore usually only achievable for small populations, which makes generalization risky. We surveyed wolverines over a large area of southern British Columbia and Alberta, Canada, used spatial capture‐recapture models to estimate density, and calculated trapping kill rates using provincial fur harvest data. Wolverine density averaged 2 wolverines/1,000 km2 and was positively related to spring snow cover and negatively related to road density. Observed annual trapping mortality was >8.4%/year. This level of mortality is unlikely to be sustainable except in rare cases where movement rates are high among sub‐populations and sizable un‐trapped refuges exist. Our results suggest wolverine trapping is not sustainable because our study area was fragmented by human and natural barriers and few large refuges existed. We recommend future wolverine trapping mortality be reduced by ≥50% throughout southern British Columbia and Alberta to promote population recovery. © 2019 The Authors. The Journal of Wildlife Management published by Wiley Periodicals, Inc. on behalf of The Wildlife Society.