bluuebunny/crossref_metadata_2025_split huggingface dataset:
BetweenSollenandSein: The CJEU’s reliance on international law in the interpretation of economic agreements covering occupied territories
Authors: Kassoti Eva 
Published: March 2020

AbstractThis contribution focuses on the Court of Justice of the European Union (Court or CJEU) reliance on international law in cases involving economic agreements covering occupied territories. In its earlier case law, the Court adopted a formalistic approach by ignoring the broader international legal framework of the dispute in an effort to achieve conformity with international law, while at the same time avoiding being drawn into political storms. The article continues by identifying an even more worrisome trend in the Court’s latest judgments in theFront PolisarioandWestern Sahara Campaign UKcases. In these two cases the Court showed its willingness to stretch the international rules of treaty interpretation to a breaking point in order to avoid pronouncing on the politically sensitive question of the de facto application of the EU’s agreements with Morocco in the territory of Western Sahara. The article concludes by asserting that the Court’s line of argumentation brings another dimension to theVölkerrechtsfreundlichkeitdebate. The classical, binary understanding of the Court’s approach as ‘open/hostile’ to international law only provides us with a partial picture of how international law was actually used in these cases. The Court’s apparent willingness to rely on international law as a heuristic device to reinforce an outcome that radically departs from the logic and structure of international law and international legal argumentation requires a more in-depth engagement with both the content of the international law rules invoked in those judgments and with the Court’s use of such rules.
Optimization of inscribed hexagonal fractal slotted microstrip antenna using modified lightning attachment procedure optimization
Authors: Anand Rohit , Chawla Paras 
Published: March 2020

AbstractAn inscribed hexagonal fractal slotted patch antenna with some additional geometry and slots is proposed for the optimization in this paper. This research work is concerned with the optimization of this slotted fractal antenna with the help of the curve-fitting method in conjunction with the modified version of Lightning Attachment Procedure Optimization (MLAPO) technique. The data required for the curve-fitting technique and for the optimization technique have been obtained by varying some of the parameters of the proposed antenna. Different equations are developed to know the relations between these parameters of the proposed antenna. The MLAPO technique is applied thereafter to calculate the different optimized geometrical parameters to optimize the bandwidth for the proposed antenna. The optimized geometrical parameters are verified with the help of a parametric variation to justify the reliable optimization. The bandwidth obtained by the MLAPO technique has been found to be better than that obtained by PSO and normal LAPO algorithm. The prototype of the optimized antenna is fabricated and the experimental results are found to be compatible with the results obtained by simulation. The proposed optimized antenna may be utilized in various applications in C and X bands.
Evolution of the Greater Caucasus Basement and Formation of the Main Caucasus Thrust, Georgia
Authors: Vasey Dylan A. , Cowgill Eric , Roeske Sarah M. , Niemi Nathan A. , Godoladze Tea , Skhirtladze Irakli , Gogoladze Salome 
Published: March 2020

AbstractAlong the northern margin of the Arabia‐Eurasia collision zone in the western Greater Caucasus, the Main Caucasus Thrust (MCT) juxtaposes Paleozoic crystalline basement to the north against Mesozoic metasedimentary and volcaniclastic rocks to the south. The MCT is commonly assumed to be the trace of an active plate‐boundary scale structure that accommodates Arabia‐Eurasia convergence, but field data supporting this interpretation are equivocal. Here we investigate the deformation history of the rocks juxtaposed across the MCT in Georgia using field observations, microstructural analysis, U‐Pb and40Ar/39Ar geochronology, and40Ar/39Ar and (U‐Th)/He thermochronology. Zircon U‐Pb analyses show that Greater Caucasus crystalline rocks formed in the Early Paleozoic on the margin of Gondwana. Low‐pressure/temperature amphibolite‐facies metamorphism of these metasedimentary rocks and associated plutonism likely took place during Carboniferous accretion onto the Laurussian margin, as indicated by igneous and metamorphic zircon U‐Pb ages of ~330–310 Ma.40Ar/39Ar ages of ~190–135 Ma from muscovite in a greenschist‐facies shear zone indicate that the MCT likely developed during Mesozoic inversion and/or rifting of the Caucasus Basin. A Mesozoic40Ar/39Ar biotite age with release spectra indicating partial resetting and Cenozoic (<40 Ma) apatite and zircon (U‐Th)/He ages imply at least ~5–8 km of Greater Caucasus basement exhumation since ~10 Ma in response to Arabia‐Eurasia collision. Cenozoic reactivation of the MCT may have accommodated a fraction of this exhumation. However, Cenozoic zircon (U‐Th)/He ages in both the hanging wall and footwall of the MCT require partitioning a substantial component of this deformation onto structures to the south.
Preseismic Fault Creep and Elastic Wave Amplitude Precursors Scale With Lab Earthquake Magnitude for the Continuum of Tectonic Failure Modes
Authors: Shreedharan Srisharan , Bolton David Chas , Rivière Jacques , Marone Chris 
Published: March 2020

AbstractTectonic faults fail in a continuum of modes from slow earthquakes to elastodynamic rupture. Precursory variations in elastic wavespeed and amplitude, interpreted as indicators of imminent failure, have been observed in limited natural settings and lab experiments where they are thought to arise from contact rejuvenation and microcracking within and around the fault zone. However, the physical mechanisms and connections to fault creep are poorly understood. Here we vary loading stiffness during frictional shear to generate a range of slip modes and measure fault zone properties using transmitted elastic waves. We find that elastic wave amplitudes show clear changes before fault failure. The temporal onset of amplitude reduction scales with lab earthquake magnitude and the magnitude of this reduction varies with fault slip. Our data provide clear evidence of precursors to lab earthquakes and suggest that continuous seismic monitoring could be useful for assessing fault state and seismic hazard potential.
Opto-thermoelectric pulling of light-absorbing particles
Authors: Lin Linhan , Kollipara Pavana Siddhartha , Kotnala Abhay , Jiang Taizhi , Liu Yaoran , Peng Xiaolei , Korgel Brian A. , Zheng Yuebing 
Published: March 2020

AbstractOptomechanics arises from the photon momentum and its exchange with low-dimensional objects. It is well known that optical radiation exerts pressure on objects, pushing them along the light path. However, optical pulling of an object against the light path is still a counter-intuitive phenomenon. Herein, we present a general concept of optical pulling—opto-thermoelectric pulling (OTEP)—where the optical heating of a light-absorbing particle using a simple plane wave can pull the particle itself against the light path. This irradiation orientation-directed pulling force imparts self-restoring behaviour to the particles, and three-dimensional (3D) trapping of single particles is achieved at an extremely low optical intensity of 10−2 mW μm−2. Moreover, the OTEP force can overcome the short trapping range of conventional optical tweezers and optically drive the particle flow up to a macroscopic distance. The concept of self-induced opto-thermomechanical coupling is paving the way towards freeform optofluidic technology and lab-on-a-chip devices.
NOD2 inhibits tumorigenesis and increases chemosensitivity of hepatocellular carcinoma by targeting AMPK pathway
Authors: Ma Xiaomin , Qiu Yumin , Sun Yanlin , Zhu Lihui , Zhao Yunxue , Li Tao , Lin Yueke , Ma Dapeng , Qin Zhenzhi , Sun Caiyu , Han Lihui 
Published: March 2020

AbstractNucleotide binding oligomerization domain 2 (NOD2) is a recognized innate immune sensor which can initiate potent immune response against pathogens. Many innate immune sensors have been reported to be of great importance in carcinogenesis. However, the role of NOD2 in cancer is not well understood. Here we investigated the role of NOD2 in the development of hepatocellular carcinoma (HCC). We demonstrated that NOD2 deficiency promoted hepatocarcinogenesis in N-nitrosodiethylamine (DEN)/carbon tetrachloride (CCl4) induced HCC mice model and xenograft tumor model. In vitro investigation showed that NOD2 acted as a tumor suppressor and inhibited proliferation, colony formation and invasion of HCC cells. Clinical investigation showed that NOD2 expression was completely lost or significantly downregulated in clinical HCC tissues, and loss of NOD2 expression was significantly correlated with advanced disease stages. Further investigation showed that NOD2 exerted its anti-tumor effect through activating adenosine 5′-monophosphate (AMP) -activated protein kinase (AMPK) signaling pathway, and NOD2 significantly enhanced the sensitivity of HCC cells to sorafenib, lenvatinib and 5-FU treatment through activating AMPK pathway induced apoptosis. Moreover, we demonstrated that NOD2 activated AMPK pathway by directly binding with AMPKα-LKB1 complex, which led to autophagy-mediated apoptosis of HCC cells. Altogether, this study showed that NOD2 acted as a tumor suppressor as well as a chemotherapeutic regulator in HCC cells by directly activating AMPK pathway, which indicated a potential therapeutic strategy for HCC treatment by upregulating NOD2-AMPK signaling axis.
Combination therapy with mTOR kinase inhibitor and dasatinib as a novel therapeutic strategy for vestibular schwannoma
Authors: Sagers Jessica E. , Beauchamp Roberta L. , Zhang Yanling , Vasilijic Sasa , Wu Limeng , DeSouza Patrick , Seist Richard , Zhou Wenjianlong , Xu Lei , Ramesh Vijaya , Stankovic Konstantina M. 
Published: March 2020

AbstractNeurofibromatosis type 2 (NF2) is an inherited disorder characterized by bilateral vestibular schwannomas (VS) that arise from neoplastic Schwann cells (SCs). NF2-associated VSs are often accompanied by meningioma (MN), and the majority of NF2 patients show loss of the NF2 tumor suppressor. mTORC1 and mTORC2-specific serum/glucocorticoid-regulated kinase 1 (SGK1) are constitutively activated in MN with loss of NF2. In a recent high-throughput kinome screen in NF2-null human arachnoidal and meningioma cells, we showed activation of EPH RTKs, c-KIT, and SFK members independent of mTORC1/2 activation. Subsequently, we demonstrated in vitro and in vivo efficacy of combination therapy with the dual mTORC1/2 inhibitor AZD2014 and the multi-kinase inhibitor dasatinib. For these reasons, we investigated activated mTORC1/2 and EPH receptor-mediated signaling in sporadic and NF2-associated VS. Using primary human VS cells and a mouse allograft model of schwannoma, we evaluated the dual mTORC1/2 inhibitor AZD2014 and the tyrosine kinase inhibitor dasatinib as monotherapies and in combination. Escalating dose-response experiments on primary VS cells grown from 15 human tumors show that combination therapy with AZD2014 and dasatinib is more effective at reducing metabolic activity than either drug alone and exhibits a therapeutic effect at a physiologically reasonable concentration (~0.1 µM). In vivo, while AZD2014 and dasatinib each inhibit tumor growth alone, the effect of combination therapy exceeds that of either drug. Co-targeting the mTOR and EPH receptor pathways with these or similar compounds may constitute a novel therapeutic strategy for VS, a condition for which there is no FDA-approved pharmacotherapy.
Humans use Optokinetic Eye Movements to Track Waypoints for Steering
Authors: Lappi Otto , Pekkanen Jami , Rinkkala Paavo , Tuhkanen Samuel , Tuononen Ari , Virtanen Juho-Pekka 
Published: March 2020

AbstractIt is well-established how visual stimuli and self-motion in laboratory conditions reliably elicit retinal-image-stabilizing compensatory eye movements (CEM). Their organization and roles in natural-task gaze strategies is much less understood: are CEM applied in active sampling of visual information in human locomotion in the wild? If so, how? And what are the implications for guidance? Here, we directly compare gaze behavior in the real world (driving a car) and a fixed base simulation steering task. A strong and quantifiable correspondence between self-rotation and CEM counter-rotation is found across a range of speeds. This gaze behavior is “optokinetic”, i.e. optic flow is a sufficient stimulus to spontaneously elicit it in naïve subjects and vestibular stimulation or stereopsis are not critical. Theoretically, the observed nystagmus behavior is consistent with tracking waypoints on the future path, and predicted by waypoint models of locomotor control - but inconsistent with travel point models, such as the popular tangent point model.
Carbohydrate response element binding protein (ChREBP) correlates with colon cancer progression and contributes to cell proliferation
Authors: Lei Yu , Zhou Shuling , Hu Qiaoling , Chen Xueling , Gu Jiang 
Published: March 2020

AbstractCancers are characterized by reprogrammed glucose metabolisms to fuel cell growth and proliferation. Carbohydrate response element binding protein (ChREBP) is a glucose-mediated transcription factor that strongly regulates glycolytic and lipogenic pathways. It has been shown to associate with metabolic diseases, such as obesity, diabetes and non-alcoholic fatty liver diseases. However, how it associates with cancers has not been well understood. In this study, ChREBP expression was assessed by immunohistochemistry in colon tissue arrays containing normal colon tissue and cancer tissue at different clinical stages. Tissue mRNA levels of ChREBP were also measured in a cohort of colon cancer patients. We found that ChREBP mRNA and protein expression were significantly increased in colon cancer tissue compared to healthy colon (p < 0.001), and their expression was positively correlated to colon malignancy (for mRNA, p = 0.002; for protein p < 0.001). Expression of lipogenic genes (ELOVL6 and SCD1) in colon cancer was also positively associated with colon malignancy (for both genes, p < 0.001). In vitro, ChREBP knockdown with siRNA transfection inhibited cell proliferation and induced cell cycle arrest without changes in apoptosis in colon cancer cell lines (HT29, DLD1 and SW480). Glycolytic and lipogenic pathways were inhibited but the p53 pathway was activated after ChREBP knockdown. Taken together, ChREBP expression is associated with colon malignancy and it might contribute to cell proliferation via promoting anabolic pathways and inhibiting p53. In addition, ChREBP might represent a novel clinical useful biomarker to evaluate the malignancy of colon cancer.
NBIA: a network-based integrative analysis framework – applied to pathway analysis
Authors: Nguyen Tin , Shafi Adib , Nguyen Tuan-Minh , Schissler A. Grant , Draghici Sorin 
Published: March 2020

AbstractWith the explosion of high-throughput data, effective integrative analyses are needed to decipher the knowledge accumulated in biological databases. Existing meta-analysis approaches in systems biology often focus on hypothesis testing and neglect real expression changes, i.e. effect sizes, across independent studies. In addition, most integrative tools completely ignore the topological order of gene regulatory networks that hold key characteristics in understanding biological processes. Here we introduce a novel meta-analysis framework, Network-Based Integrative Analysis (NBIA), that transforms the challenging meta-analysis problem into a set of standard pathway analysis problems that have been solved efficiently. NBIA utilizes techniques from classical and modern meta-analysis, as well as a network-based analysis, in order to identify patterns of genes and networks that are consistently impacted across multiple studies. We assess the performance of NBIA by comparing it with nine meta-analysis approaches: Impact Analysis, GSA, and GSEA combined with classical meta-analysis methods (Fisher’s and the additive method), plus the three MetaPath approaches that employ multiple datasets. The 10 approaches have been tested on 1,737 samples from 27 expression datasets related to Alzheimer’s disease, acute myeloid leukemia (AML), and influenza. For all of the three diseases, NBIA consistently identifies biological pathways relevant to the underlying diseases while the other 9 methods fail to capture the key phenomena. The identified AML signature is also validated on a completely independent cohort of 167 AML patients. In this independent cohort, the proposed signature identifies two groups of patients that have significantly different survival profiles (Cox p-value 2 × 10−6). The NBIA framework will be included in the next release of BLMA Bioconductor package (http://bioconductor.org/packages/release/bioc/html/BLMA.html).
A new multigene HCIQ subfamily from the sea anemone Heteractis crispa encodes Kunitz-peptides exhibiting neuroprotective activity against 6-hydroxydopamine
Authors: Kvetkina Aleksandra , Leychenko Elena , Chausova Victoria , Zelepuga Elena , Chernysheva Nadezhda , Guzev Konstantin , Pislyagin Evgeny , Yurchenko Ekaterina , Menchinskaya Ekaterina , Aminin Dmitry , Kaluzhskiy Leonid , Ivanov Alexis , Peigneur Steve , Tytgat Jan , Kozlovskaya Emma , Isaeva Marina 
Published: March 2020

AbstractThe Kunitz/BPTI-type peptides are ubiquitous in numerous organisms including marine venomous animals. The peptides demonstrate various biological activities and therefore they are the subject of a number of investigations. We have discovered a new HCIQ subfamily belonging to recently described multigene HCGS family of Heteractis crispa Kunitz-peptides. The uniqueness of this subfamily is that the HCIQ precursors contain a propeptide terminating in Lys-Arg (endopeptidase cleavage site) the same as in the neuro- and cytotoxin ones. Moreover, the HCIQ genes contain two introns in contrast to HCGS genes with one intron. As a result of Sanger and amplicon deep sequencings, 24 HCIQ isoforms were revealed. The recombinant peptides for the most prevalent isoform (HCIQ2c1) and for the isoform with the rare substitution Gly17Glu (HCIQ4c7) were obtained. They can inhibit trypsin with Ki 5.2 × 10−8 M and Ki 1.9 × 10−7 M, respectively, and interact with some serine proteinases including inflammatory ones according to the SPR method. For the first time, Kunitz-peptides have shown to significantly increase neuroblastoma cell viability in an in vitro 6-OHDA-induced neurotoxicity model being a consequence of an effective decrease of ROS level in the cells.
Induced Beta Power Modulations during Isochronous Auditory Beats Reflect Intentional Anticipation before Gradual Tempo Changes
Authors: Graber Emily , Fujioka Takako 
Published: March 2020

AbstractInduced beta-band power modulations in auditory and motor-related brain areas have been associated with automatic temporal processing of isochronous beats and explicit, temporally-oriented attention. Here, we investigated how explicit top-down anticipation before upcoming tempo changes, a sustained process commonly required during music performance, changed beta power modulations during listening to isochronous beats. Musicians’ electroencephalograms were recorded during the task of anticipating accelerating, decelerating, or steady beats after direction-specific visual cues. In separate behavioural testing for tempo-change onset detection, such cues were found to facilitate faster responses, thus effectively inducing high-level anticipation. In the electroencephalograms, periodic beta power reductions in a frontocentral topographic component with seed-based source contributions from auditory and sensorimotor cortices were apparent after isochronous beats with anticipation in all conditions, generally replicating patterns found previously during passive listening to isochronous beats. With anticipation before accelerations, the magnitude of the power reduction was significantly weaker than in the steady condition. Between the accelerating and decelerating conditions, no differences were found, suggesting that the observed beta patterns may represent an aspect of high-level anticipation common before both tempo changes, like increased attention. Overall, these results indicate that top-down anticipation influences ongoing auditory beat processing in beta-band networks.
Attention and cardiac phase boost judgments of trust
Authors: Li Xinyi , Chiu Michelle , Swallow Khena M. , De Rosa Eve , Anderson Adam K. 
Published: March 2020

AbstractFluctuations in mental and bodily states have both been shown to be associated with negative affective experience. Here we examined how momentary fluctuations in attentional and cardiac states combine to regulate the perception of positive social value. Faces varying in trustworthiness were presented during a go/no-go letter target discrimination task synchronized with systolic or diastolic cardiac phase. Go trials lead to an attentional boosting of perceived trust on high trust and ambiguous neutral faces, suggesting attention both boosted existing and generated positive social value. Cardiac phase during face presentation interacted with attentional boosting of trust, enhancing high trust faces specifically during relaxed diastolic cardiac states. Confidence judgments revealed that attentional trust boosting, and its cardiac modulation, did not reflect altered perceptual or response fluency. These results provide evidence for how moment-to moment fluctuations in top-down mental and bottom-up bodily inputs combine to enhance a priori and generate de novo positive social value.
Plasmonic coupling in closed-packed ordered gallium nanoparticles
Authors: Catalán-Gómez S. , Bran C. , Vázquez M. , Vázquez L. , Pau J. L. , Redondo-Cubero A. 
Published: March 2020

AbstractPlasmonic gallium (Ga) nanoparticles (NPs) are well known to exhibit good performance in numerous applications such as surface enhanced fluorescence and Raman spectroscopy or biosensing. However, to reach the optimal optical performance, the strength of the localized surface plasmon resonances (LSPRs) must be enhanced particularly by suitable narrowing the NP size distribution among other factors. With this purpose, our last work demonstrated the production of hexagonal ordered arrays of Ga NPs by using templates of aluminium (Al) shallow pit arrays, whose LSPRs were observed in the VIS region. The quantitative analysis of the optical properties by spectroscopic ellipsometry confirmed an outstanding improvement of the LSPR intensity and full width at half maximum (FWHM) due to the imposed ordering. Here, by engineering the template dimensions, and therefore by tuning Ga NPs size, we expand the LSPRs of the Ga NPs to cover a wider range of the electromagnetic spectrum from the UV to the IR regions. More interestingly, the factors that cause this optical performance improvement are studied with the universal plasmon ruler equation, supported with discrete dipole approximation simulations. The results allow us to conclude that the plasmonic coupling between NPs originated in the ordered systems is the main cause for the optimized optical response.