bluuebunny/crossref_metadata_2025_split huggingface dataset:
RETRACTED ARTICLE: Fire and summer temperatures work together breaking physical seed dormancy
Authors: Luna Belén 
Published: April 2020

AbstractFire and high summer soil temperatures can break physical seed dormancy in Mediterranean fire-prone ecosystems. Their independent effect is somewhat recognized but both factors may act together with a synergistic effect yet unknown. This study aims to determine the isolated and combined effects of fire and summer temperatures on the release of physical seed dormancy in Cistaceae species. Fire and summer temperature treatments were applied in a factorial experiment to seeds of 12 species of Cistaceae. Seeds previously exposed or not to a heat shock (fire simulation) were kept for 1 or 2 months at constant or alternating temperatures (summer temperatures simulation). Additionally, I compared the effect of exposing the seeds to a heat shock before or after they had been subjected to the summer temperatures. Heat shock increased germination of all species, but summer temperatures produced different results. When seeds were exposed to summer temperatures after heat shock, germination decreased. This negative effect disappeared when heat shock was simulated at the end of the summer temperatures. Fire and summer temperatures modulate timing of germination in Cistaceae with a joint control on post-fire regeneration. Cycling of sensitivity to physical dormancy release may be the mechanism to explain this fine-tuning, which would ensure germination when environmental conditions are suitable for growth. These results contribute to our understanding of vegetation dynamics and postfire regeneration in Mediterranean ecosystems.
Gamma3 nail with U-Blade (RC) lag screw is effective with better surgical outcomes in trochanteric hip fractures
Authors: Han Seung-Beom , Jung Jae-Kyun , Jang Chul-Young , Kwak Dae-Kyung , Kim Jeong-Woo , Yoo Je-Hyun 
Published: April 2020

AbstractThe objective of this retrospective study was to investigate the surgical outcomes of AO/OTA 31 A1-3 trochanteric fractures treated with the new-generation Gamma3 nail with U-Blade (RC) lag screw and to analyze the risk factors related to fixation failure. A total of 318 consecutive patients who underwent cephalomedullary nailing using Gamma3 nail with U-Blade lag screw for trochanteric hip fractures between September 2015 and June 2018 were enrolled. The average age was 80 years and most patients (69%) were women. The mean follow-up was 12.2 months with a minimum of 6 months. 309 (97.2%) showed bony union with a mean time to union of 13.5 ± 8.7 weeks. Cut-out occurred in 2 patients (0.6%) and 7 patients showed excessive collapse (≥15 mm) of the proximal fragment. These 9 patients were assigned to the failure group. The presence of a basicervical fracture component and comminution of the anterior cortex on preoperative 3-D CT showed a significant association with fixation failure, including cut-out, although comminution of the anterior cortex was the only independent risk factor for fixation failure on multivariate logistic regression analysis. Gamma3 nail with U-Blade lag screw showed favorable results for trochanteric hip fractures, with low cut-out rate (0.6%). However, more caution is required in treating trochanteric fractures with a basicervical fracture component and anterior cortex comminution even with this nail.
Sprifermin (rhFGF18) versus vehicle induces a biphasic process of extracellular matrix remodeling in human knee OA articular cartilage ex vivo
Authors: Reker D. , Siebuhr A. S. , Thudium C. S. , Gantzel T. , Ladel C. , Michaelis M. , Aspberg A. , Berchtold M. W. , Karsdal M. A. , Gigout A. , Bay-Jensen A. C. 
Published: April 2020

AbstractSprifermin, recombinant human fibroblast growth factor 18 (rhFGF18), induces cartilage regeneration in knees of patients with osteoarthritis (OA). We hypothesized that a temporal multiphasic process of extracellular matrix (ECM) degradation and formation underlie this effect. We aimed to characterize the temporal ECM remodeling of human knee OA articular cartilage in response to sprifermin treatment. Articular cartilage explants from patients with knee OA (npatients = 14) were cultured for 70 days, with permanent exposure to sprifermin (900, 450, 225 ng/mL), FGF18 (450 ng/mL), insulin-like growth factor-1 (100 ng/mL, positive control) or vehicle (nreplicates/treatment/patient = 2). Metabolic activity (AlamarBlue) and biomarkers of type IIB collagen (PIIBNP) formation (Pro-C2 enzyme-linked immunosorbent assay [ELISA]) and aggrecanase-mediated aggrecan neo-epitope NITEGE (AGNx1 ELISA) were quantified once a week. At end of culture (day 70), gene expression (quantitative reverse transcription polymerase chain reaction) and proteoglycan content (Safranin O/Fast green staining) were quantified. The cartilage had continuously increased metabolic activity, when treated with sprifermin/FGF18 compared to vehicle. During days 7–28 PIIBNP was decreased and NITEGE was increased, and during days 35–70 PIIBNP was increased. At end of culture, the cartilage had sustained proteoglycan content and relative expression of ACAN < COL2A1 < SOX9 < COL1A1, indicating that functional chondrocytes remained in the explants. Sprifermin induces a temporal biphasic cartilage remodeling in human knee OA articular cartilage explants, with early-phase increased aggrecanase activity and late-phase increased type II collagen formation.
SMRT sequencing of the Oryza rufipogon genome reveals the genomic basis of rice adaptation
Authors: Li Wei , Li Kui , Huang Ying , Shi Cong , Hu Wu-Shu , Zhang Yun , Zhang Qun-Jie , Xia En-Hua , Hutang Ge-Ran , Zhu Xun-Ge , Liu Yun-Long , Liu Yuan , Tong Yan , Zhu Ting , Huang Hui , Dan Zhang  , Zhao Yuan , Jiang Wen-Kai , Yuan Jie , Niu Yong-Chao , Gao Cheng-Wen , Gao Li-Zhi 
Published: April 2020

AbstractAsian cultivated rice is believed to have been domesticated from a wild progenitor, Oryza rufipogon, offering promising sources of alleles for world rice improvement. Here we first present a high-quality chromosome-scale genome of the typical O. rufipogon. Comparative genomic analyses of O. sativa and its two wild progenitors, O. nivara and O. rufipogon, identified many dispensable genes functionally enriched in the reproductive process. We detected millions of genomic variants, of which large-effect mutations could affect agronomically relevant traits. We demonstrate how lineage-specific expansion of gene families may have contributed to the formation of reproduction isolation. We document thousands of genes with signatures of positive selection that are mainly involved in the reproduction and response to biotic- and abiotic stresses. We show that selection pressures may serve as forces to govern substantial genomic alterations that form the genetic basis of rapid evolution of mating and reproductive systems under diverse habitats.
The [4Fe-4S] cluster of sulfurtransferase TtuA desulfurizes TtuB during tRNA modification in Thermus thermophilus
Authors: Chen Minghao , Ishizaka Masato , Narai Shun , Horitani Masaki , Shigi Naoki , Yao Min , Tanaka Yoshikazu 
Published: April 2020

AbstractTtuA and TtuB are the sulfurtransferase and sulfur donor proteins, respectively, for biosynthesis of 2-thioribothymidine (s2T) at position 54 of transfer RNA (tRNA), which is responsible for adaptation to high temperature environments in Thermus thermophilus. The enzymatic activity of TtuA requires an iron-sulfur (Fe-S) cluster, by which a sulfur atom supplied by TtuB is transferred to the tRNA substrate. Here, we demonstrate that the Fe-S cluster directly receives sulfur from TtuB through its inherent coordination ability. TtuB forms a [4Fe-4S]-TtuB intermediate, but that sulfur is not immediately released from TtuB. Further desulfurization assays and mutation studies demonstrated that the release of sulfur from the thiocarboxylated C-terminus of TtuB is dependent on adenylation of the substrate tRNA, and the essential residue for TtuB desulfurization was identified. Based on these findings, the molecular mechanism of sulfur transfer from TtuB to Fe-S cluster is proposed.
Acetylation of histone H3K27 signals the transcriptional elongation for estrogen receptor alpha
Authors: Gao Yujing , Chen Lijia , Han Yali , Wu Fangrui , Yang Wen-Si , Zhang Zheng , Huo Tong , Zhu Yingmin , Yu Chengtai , Kim Hong , Lee Mark , Tang Zhen , Phillips Kevin , He Bin , Jung Sung Yun , Song Yongcheng , Zhu Bokai , Xu Rui-Ming , Feng Qin 
Published: April 2020

AbstractAs approximately 70% of human breast tumors are estrogen receptor α (ERα)-positive, estrogen and ERα play essential roles in breast cancer development. By interrupting the ERα signaling pathway, endocrine therapy has been proven to be an effective therapeutic strategy. In this study, we identified a mechanism by which Transcription Start Site (TSS)-associated histone H3K27 acetylation signals the Super Elongation Complex (SEC) to regulate transcriptional elongation of the ESR1 (ERα) gene. SEC interacts with H3K27ac on ESR1 TSS through its scaffold protein AFF4. Depletion of AFF4 by siRNA or CRISPR/Cas9 dramatically reduces expression of ESR1 and its target genes, consequently inhibiting breast cancer cell growth. More importantly, a AFF4 mutant which lacks H3K27ac interaction failed to rescue ESR1 gene expression, suggesting H3K27 acetylation at TSS region is a key mark bridging the transition from transcriptional initiation to elongation, and perturbing SEC function can be an alternative strategy for targeting ERα signaling pathway at chromatin level.
Morphological Errors in Monolingual Spanish-Speaking Children With and Without Developmental Language Disorders
Authors: Castilla-Earls Anny , Auza Alejandra , Pérez-Leroux Ana Teresa , Fulcher-Rood Katrina , Barr Christopher 
Published: April 2020

Purpose
The purpose of this study was to identify which morphological markers have the best diagnostic accuracy to identify developmental language disorders (DLD) in monolingual Spanish-speaking children.

Method

The participants in this study included 50 Spanish-speaking monolingual children with (
n
= 25) and without (
n
= 25) DLD. Data collection took place in Mexico. Children were administered a comprehensive elicitation task that set up felicitous contexts to produce morphological structures previously identified as problematic for Spanish-speaking children with DLD: articles, direct object pronouns, adjectives, plurals, verb conjugations, and the subjunctive in Spanish.

Results
Statistically significant group differences between children with and without DLD were found for all morphological structures examined but plurals. Logistic regression analyses suggested that a model that included clitic and verbs was the best model to uniquely predict group membership. This model showed sensitivity of 96% and specificity of 80%.

Conclusion
Clitics and verbs should be considered morphological markers of DLD in monolingual Spanish-speaking children.
Hearing Aid Amplification Reduces Communication Effort of People With Hearing Impairment and Their Conversation Partners
Authors: Beechey Timothy , Buchholz Jörg M. , Keidser Gitte 
Published: April 2020

Objectives
This study investigates the hypothesis that hearing aid amplification reduces effort within conversation for both hearing aid wearers and their communication partners. Levels of effort, in the form of speech production modifications, required to maintain successful spoken communication in a range of acoustic environments are compared to earlier reported results measured in unaided conversation conditions.

Design
Fifteen young adult normal-hearing participants and 15 older adult hearing-impaired participants were tested in pairs. Each pair consisted of one young normal-hearing participant and one older hearing-impaired participant. Hearing-impaired participants received directional hearing aid amplification, according to their audiogram, via a master hearing aid with gain provided according to the NAL-NL2 fitting formula. Pairs of participants were required to take part in naturalistic conversations through the use of a referential communication task. Each pair took part in five conversations, each of 5-min duration. During each conversation, participants were exposed to one of five different realistic acoustic environments presented through highly open headphones. The ordering of acoustic environments across experimental blocks was pseudorandomized. Resulting recordings of conversational speech were analyzed to determine the magnitude of speech modifications, in terms of vocal level and spectrum, produced by normal-hearing talkers as a function of both acoustic environment and the degree of high-frequency average hearing impairment of their conversation partner.

Results
The magnitude of spectral modifications of speech produced by normal-hearing talkers during conversations with aided hearing-impaired interlocutors was smaller than the speech modifications observed during conversations between the same pairs of participants in the absence of hearing aid amplification.

Conclusions
The provision of hearing aid amplification reduces the effort required to maintain communication in adverse conditions. This reduction in effort provides benefit to hearing-impaired individuals and also to the conversation partners of hearing-impaired individuals. By considering the impact of amplification on both sides of dyadic conversations, this approach contributes to an increased understanding of the likely impact of hearing impairment on everyday communication.
Precise multi‐dimensional temperature‐rise characterisation of switchgear based on multi‐conditional experiments and LPTN model for high‐capacity application
Authors: Wang Xiaorui , Xia Haotian , Yu Zhanqing , Guan Yonggang , Zhao Biao , Huang Yulong , Qu Lu , Ding Jiudong 
Published: April 2020

AbstractAs the power load increases, the capacity of switchgear becomes larger so the problem of overheating of switchgears becomes more significant. Whether the switchgears operate safely and reliably affects the reliability and economy of the entire power system. Different methods have been tried to study the thermal problems of switchgears. However, there are few experimental studies on the switchgear entity. In this study, a series of temperature rise tests were carried out on medium‐voltage high‐capacity switchgear to explore laws of temperature rise. First, five groups of multi‐conditional experiments were carried out including changes of the load current, ventilation conditions and loop resistance. Then, multi‐dimensional temperature‐rise characterisation was analysed based on the experimental results and the relating theory. Finally, a circuit‐based lumped‐parameter thermal network (LPTN) model was developed by analysing the heat dissipation in switchgear and used to determine the steady‐state temperature distribution of the switchgear. The model is verified by comparing the simulation results with the experimental results.
Testing cable system reliability after commissioning and during operation life: in medio stat virtus?
Authors: Montanari Gian Carlo , Seri Paolo , Ghosh Riddhi , Franchi Bononi Stefano , Albertini Marco 
Published: April 2020

AbstractQuality control, commissioning and diagnostic tests on insulation systems of electrical apparatus are necessary to reduce the failure risk under operation, eliminate infant mortality failures and reduce the impact of ageing on insulation system reliability. This study tries to establish a path which, based on the statistical–phenomenological modelling of the hazard rate, or bathtub curve, drives in the choice of test overvoltage and procedures. The choice of the test voltage and its waveform should be made carefully to improve reliability (reduce failure hazard) in any section of the bathtub curve. This may require, however, different choices depending on the section which corresponds to the planned test. For example, large overvoltages could be acceptable to remove the infant mortality to ensure the highest reliability of newly commissioned apparatus, but they can incept highly energetic, and, thus, fast degradation mechanisms on apparatus operating on the field for a period of time, that can worsen, rather than improving, the insulation system reliability. Resorting to partial discharge measurement during any overvoltage test seems to be the optimal solution to find the most appropriate overvoltage for any type of testing need, leveraging the Latin concept that ‘in medio stat virtus’.
Lipotransfer führt zu Verbesserung von Schluck- und Artikulationsstörungen nach mikrochirurgischer Zungenrekonstruktion – ein Fallbericht
Authors: Cai Aijia , Iro Heinrich , Horch Raymund E 
Published: April 2020

ZusammenfassungNach mikrochirurgischer Zungenrekonstruktion durch freie Lappenplastiken kommt es häufig zu einer Atrophie der Zunge, was zu funktionellen Einschränkungen wie Artikulationsstörungen oder Dysphagie führt. Eine Volumenaugmentation mittels Lipotransfer könnte dieser Problematik entgegenwirken. Wir beschreiben einen Fallbericht über wiederholte Lipotransfers in eine atrophierte rekonstruierte Zunge. Ein 66-jähriger Patient hatte bei Plattenepithelkarzinom der Zunge eine Hemiglossektomie und die anschließende Zungenrekonstruktion mittels freier Radialislappenplastik erhalten. Nach Atrophie der rekonstruierten Zunge führten wir wiederholte Lipotransfer-Sitzungen zur Augmentation des atrophierten Gewebes durch. Insgesamt führten drei Lipotransfer-Sitzungen zu einem zufriedenstellenden Ergebnis. Durch die Augmentation der Zunge konnte eine Verbesserung der ursprünglichen Schluck- und Artikulationsstörungen erzielt werden.Dies ist nach unserem Kenntnisstand der erste Fallbericht über Augmentation eines Lappens in der Mundhöhle mittels Lipotransfer, welches ein nützliches Mittel bei der Zungenrekonstruktion darstellt. Wir empfehlen daher die Volumenaugmentation mittels Lipotransfer als zusätzliches Hilfsmittel zur Verbesserung des funktionellen Status bei der Zungenrekonstruktion.
Aktueller Algorithmus zur Diagnostik und Therapie des Brustimplantat-assoziierten anaplastischen großzelligen Lymphoms
Authors: Moellhoff Nicholas , Frank Konstantin , Wiggenhauser Paul S. , Schenck Thilo L. , Mayr Doris , Giunta Riccardo E. 
Published: April 2020

ZusammenfassungBei elektiven Operationen gilt es ein höchstes Maß an Sicherheit zu gewährleisten und Patienten ausführlich über die mit dem Eingriff verbundenen Risiken zu informieren. Insbesondere bei ästhetischen Eingriffen in der Plastischen Chirurgie ist es die Pflicht des Chirurgen eine ganzheitliche und umfassende Aufklärung auch über mögliche Langzeitfolgen durchzuführen. In den letzten Jahren steht die Verwendung von Brustimplantaten zur ästhetischen Brustaugmentation oder zur Brustrekonstruktion nach Tumorresektion zunehmend im Fokus der Öffentlichkeit, nachdem sich weltweit die Fallzahlen von seltenen Brustimplantat-assoziierten Krankheitsbildern häufen und die Ursachen nicht vollständig geklärt sind. Das breast implant-associated anaplastic large cell lymphoma (engl. BIA-ALCL) wurde 2016 von der Weltgesundheitsorganisation als lymphatische Neoplasie klassifiziert.Mittlerweile wurden weltweit knapp 800 Fälle, die vor allem texturierte Implantate betreffen, dokumentiert. Die maligne Erkrankung führt zu einer Verunsicherung von Patientinnen mit Implantaten. Daher ist es eine zentrale Aufgabe der Plastischen Chirurgen dieses seltene Krankheitsbild zu verstehen, es frühzeitig zu erkennen und klare Handlungsempfehlungen zu formulieren. Das vorliegende Manuskript soll den aktuellen Stand zum Vorgehen im Hinblick auf Diagnostik und Therapie des BIA-ALCL zusammenfassen.
Wie gut werden Patientinnen auf Internetseiten von deutschen universitären Brustzentren über die Möglichkeiten der Brustrekonstruktion informiert?
Authors: Keck Maike , Bergmann Philipp Alexander , Deindl Philipp , Wittig Klaus Stephan , Lohmeyer Jörn Andreas 
Published: April 2020

Zusammenfassung
Einleitung Das Internet hat sich in den letzten Jahrzehnten zu einer der wichtigsten Informationsquellen für Patienten entwickelt. Wie gut sind die Möglichkeiten, sich im Internet über eine Brustrekonstruktion zu informieren? Wie präsent ist die Plastische Chirurgie mit ihrem Spektrum an modernen rekonstruktiven Möglichkeiten in Deutschland?
Methoden Insgesamt wurden 35 Homepages der universitären Brustzentren in Deutschland bezüglich Erwähnung einer Zusammenarbeit mit einem Plastischen Chirurgen bzw. einer Abteilung für Plastische Chirurgie und deren Verlinkung, Angaben bezüglich rekonstruktiver Möglichkeiten wie der Implantatrekonstruktion, Eigengeweberekonstruktion mit gestielten und freien Lappenplastiken sowie Lipofilling analysiert.
Ergebnisse Auf 49 % der Homepages wird ein Plastischer Chirurg bzw. eine kooperierende Abteilung erwähnt, 20 % beinhalten eine direkte Verlinkung. Auf 91 % der Seiten wird die Möglichkeit einer Brustrekonstruktion beschrieben, auf 80 % der Webseiten die Verwendung von Eigengewebe im Allgemeinen. In 51 % der Fälle wird ein freier Gewebetransfer erwähnt, wobei nur 23 % die Verfahren wie DIEP-Lappenplastik, TMG oder I-GAP genauer beschreiben. Nur zwei Zentren verwenden hierzu detaillierte Abbildungen. Bei sechs Internetauftritten (17 %) wird lediglich eine Rekonstruktion mit gestieltem TRAM Lappen beschrieben. Eine Rekonstruktion mit Silikonimplantat wird auf 71 % der Websites erwähnt. Die Möglichkeit eines Lipofillings wird in 31 % erörtert.
Diskussion In etwa der Hälfe der Webauftritte universitärer Brustzentren ist keine Zusammenarbeit mit einem Plastischen Chirurgen ersichtlich. Die Informationen über den freien Gewebetransfer zur Brustrekonstruktion weisen große Lücken auf.
Large Fibre Neuropathy: Part of Fibromyalgia or Coexisting
Entity?
Authors: Gezginaslan Ömer , Sürmeli Reyhan , Atalay Sevgi Gümüş 
Published: April 2020

Zusammenfassung
Zielsetzung Ziel dieser Studie ist es, die Inzidenz von Large Fibre
Neuropathy (LFN) bei Fibromyalgie (FM) -Patienten mit peripheren
Neuropathie-Beschwerden zu untersuchen und den Einfluss von LFN und FM auf
Schmerzen, Lebensqualität, Schlafqualität, Behinderung, und
depressive Symptome.
Methoden Zwischen Juni 2018 und Februar 2019 wurden insgesamt 104
Patienten (67 Frauen, 37 Männer; Durchschnittsalter: 52,21±9,53
Jahre; Spanne 31–74 Jahre) mit der Diagnose FM in die Studie
eingeschlossen. Die Patienten wurden in 2 Gruppen eingeteilt: Gruppe 1,
einschließlich Patienten mit Polyneuropathie (PNP) (n=48) und
Gruppe 2, einschließlich Nicht-PNP-Patienten (n=54). Gruppe 1
wurde auch weiter in 2 Untergruppen als sensorische PNP (n=28) und
sensomotorische PNP (n=20) unterteilt. Alle Patienten wurden anhand der
Visual Analog Scale (VAS), des Fibromyalgia Impact Questionnaire (FIQ), des
Short Form-36 (SF-36), des Pittsburgh Sleep Quality Index (PSQI) und des Beck
Depression Inventory (BDI) bewertet.
Ergebnisse Es gab keinen statistisch signifikanten Unterschied in den
demografischen Merkmalen zwischen den Gruppen (p>0,05). Es gab einen
statistisch signifikanten Unterschied in den VAS-, SF-36-, BDI-, FIQ- und
PSQI-Werten zwischen Gruppe 1 und Gruppe 2 (p<0,05). Es gab keinen
statistisch signifikanten Unterschied in den VAS-, SF-36-, BDI-, FIQ- und
PSQI-Werten zwischen den sensorischen und sensomotorischen PNP-Gruppen
(p>0,05).
Schlussfolgerungen Unsere Studienergebnisse zeigen, dass FM mit LFN die
Schmerzen, die Lebensqualität, die Schlafqualität, die
Behinderung und die depressiven Symptome negativ beeinflusst, gegenüber
denjenigen ohne LFN, was auf die Bedeutung des Neuropathiemanagements bei
FM-Patienten hinweist.