bluuebunny/crossref_metadata_2025_split huggingface dataset:
Construction of homologous cancer cell membrane camouflage in a nano-drug delivery system for the treatment of lymphoma
Authors: Zhao Qiangqiang , Sun Xiaoying , Wu Bin , Shang Yinghui , Huang Xueyuan , Dong Hang , Liu Haiting , Chen Wansong , GUI RONG , Li Jian 
Published: May 2020

Abstract
Background Non-Hodgkin's lymphoma (NHL) possesses great heterogeneity in cytogenetics, immunophenotype and clinical features, and chemotherapy currently serves as the main treatment modality. Although employing monoclonal antibod y targeted drugs has significantly improved its overall efficacy, various patients continue to suffer from drug resistance or recurrence. Chinese medicine has long been used in the treatment of malignant tumors. Results Accordingly, this study put forward the construction of a nano-platform based on mesoporous silica nanoparticles (MSNs) loaded with the traditional Chinese medicine isoimperatorin (ISOIM), which was camouflaged by the cancer cell membrane (CCM) called CCM@MSNs-ISOIM. The proposed nano-platform has characteristics of immune escape, anti-phagocytosis, high drug loading rate, pH response, good biocompatibility and active targeting of the tumor site, blocking the lymphoma cell cycle and promoting mitochondrial-mediated apoptosis.Conclusions Furthermore, this study provides a theoretical basis in finding novel clinical treatments for lymphoma.
Micelles self-assembled by 3-O-β-D-glucopyranosyl latycodigenin enhances cell membrane permeability, promotes antibiotic pulmonary targeting and improves anti-infect efficacy
Authors: Zhang Man , Ye Lili , Huang Hao , Cheng Dandan , Wu Wenbo , Liu Kaixin , Jiang Zhihong , Hou Yuanyuan , Bai Gang 
Published: May 2020

Abstract
Background: Nanoparticle-based pulmonary drug delivery systems are commonly developed and applied for drug-targeted delivery. It exhibits significant advantages compared to traditional pulmonary drug delivery systems. However, developing a formulation for each drug is a time-consuming and laborious task. Results: The present study designed and constructed a universal lung-targeting nanoparticle. The self-assembled micelles were composed of a platycodon secondary saponin, 3-O-β-D-glucopyranosyl platycodigenin 682 (GP-682), via its specific amphiphilic structure. After optimization, the GP-682 micelles obtained a stable zeta potential with a particle size between 60 to 90 nm, and the CMC value was approximately 42.3 μg/mL. Pre-incubation of GP-682 micelles markedly enhanced the cell membrane permeability, and improved drug uptake in vitro . The results were visualized using fluorescent dye tracing, transmission electron microscopy observation and a lactate dehydrogenase releasing assay. The benefits enhanced the distribution of levofloxacin in mouse lung tissue and reduced the overdosing of antibiotic. Pseudomonas aeruginosa PA 14 strain-induced acute lung injury mice model demonstrated that preinjection of GP-682 micelles followed by antibiotic administration produced a higher survival rate and anti-infection efficacy in vivo , which included a reduction in pulmonary injury, bacterial invasion and the expression of cytokines compared to treatment with levofloxacin alone. Conclusions: GP-682 micelles are another nanoparticle-based pulmonary drug delivery system to increase the use of antibiotic and provide a new option for antibiotic resistance and lung-targeting therapy.
PPAI: a web server for predicting protein-aptamer interactions
Authors: Li Jianwei , Ma Xiaoyu , Li Xichuan , Gu Junhua 
Published: May 2020

Abstract
Background: The interactions between proteins and aptamers are prevalent in organisms and play an important role in various life activities. Thanks to the rapid accumulation of protein-aptamer interaction data, it is necessary and feasible to construct an accurate and effective computational model to predict aptamers binding to certain interested proteins and protein-aptamer interactions, which is beneficial for understanding mechanisms of protein-aptamer interactions and improving aptamer-based therapies. Results: In this study, a novel web server named PPAI is developed to predict aptamers and protein-aptamer interactions with key sequence features of proteins/aptamers and a machine learning framework integrated adaboost and random forest. A new method for extracting several key sequence features of both proteins and aptamers is presented, where the features for proteins are extracted from amino acid composition, pseudo-amino acid composition, grouped amino acid composition, C/T/D composition and sequence-order-coupling number, while the features for aptamers are extracted from nucleotide composition, pseudo-nucleotide composition (PseKNC) and Moreau-Broto autocorrelation coefficient. On the basis of these feature sets and balanced the samples with SMOTE algorithm, we validate the performance of PPAI by the independent test set. The results demonstrate that the Area Under Curve (AUC) is 0.907 for prediction of aptamer, while the AUC reaches 0.871 for prediction of protein-aptamer interactions. Conclusion: These results indicate that PPAI can query aptamers, predict aptamers and predict protein-aptamer interactions in batch mode precisely and efficiently, which would be a novel bioinformatics tool for the research of protein-aptamer interactions. PPAI web-server is freely available at http://39.96.85.9/PPAI.
Perceived Stress and Its Impact on Health Behavior of Chinese Residents during the Epidemic of COVID-19: An Internet Survey
Authors: Li Yuerong , Yao Lili , Luo Yetao , Yuan Feng , Yan Lupei 
Published: May 2020

Abstract
Background An outbreak of 2019 novel coronavirus disease(COVID-19) could cause a severe respiratory illness, which may come from Spray or ordinary contact, then pose a great threat to life. Meanwhile, epidemic may trigger excessive stress and have a terrible impact on mental health even health behaviors, especially some special individuals. So this study paved the way for psychological interventions focused on specific groups during the COVID-19 epidemic. Methods An internet survey was conducted on 2,449 residents in 20 provinces including Chongqing, Sichuan and Gansu et al, in order to collect the data of Chinese residents' perceived stress, COVID-19 awareness and health behaviors. SAS9.4 was used to analyze the relationship between health behaviors and perceived stress, and logistic regression was used to explore the influencing factors of health risk stress. Results The residents' perceived stress score was 22.25±7.2(total score was 56), and the incidence of health risk stress was 39.89% (977/2449). As perceived stress increased, the frequency of health behaviors such as washing hands and wearing a mask decreased in turn. Age, cognition of susceptibility to COVID-19, life-threaten level, cognition of the importance of home isolation, and cognition of the difference between common cold and COVID-19 were related to the occurrence of health risk stress. Conclusions There was a negative correlation between residents' health behaviors and perceived stress during the COVID-19 outbreak. It is of great significance to improve the public's awareness of the COVID-19, to provide good social support and psychological intervention for people with health risk stress and to promote their health behaviors.
On nature of determining displacement from sole knowledge of self interferometric perturbed phase
Authors: Khan Junaid 
Published: May 2020

Abstract
While self mixing interferometry(SMI) has proven to be suitable for displacement measurement and other sensing applications,its characteristic self mixing signal shape is strongly governed by the non-linear phase equation which forms relation between perturbed and unperturbed phase of self mixing laser.Therefore, while it is desirable for robust estimation of displacement of moving target, the algorithms to achieve this must have an objective direction which can be achieved by understanding the characteristic of extracting knowledge of perturbed phase from unperturbed phase. Therefore, this paper tends to deduce results from the self mixing interferometry(SMI) phase equation,by showing the impossibility of analytically deriving perturbed phase as continuous function of unperturbed phase either through finite or infinite substitutions. Therefore we never get exact displacement just by sole knowledge of perturbed phase. Moreover, it has theoretically shown that the action of practically deriving unperturbed phase from perturbed phase becomes a filltering process in spectral domain followed by perturbative or homotopical deformation.
Associations between socio-demographics, nutrition knowledge, nutrition competencies and attitudes in community-dwelling older adults in Singapore: Findings from the SHIELD study
Authors: Ong Rebecca Hui Shan , Chow Wai Leng , Cheong Magdalin , Lim Gladys Huiyun , Xie Weiyi , Baggs Geraldine , Huynh Dieu Thi Thu , Oh Hong Choon , How Choon How , Tan Ngiap-Chuan , Tey Siew Ling , Chew Samuel Teong Huang 
Published: May 2020

Abstract
Background Nutrition literacy refers to an individual’s knowledge, motivation and competencies to access, process, and understand nutrition information to make nutrition-related decisions. It is known to influence dietary habits of older adults. This cross-sectional study was designed to: (1) understand the nutrition knowledge, competencies and attitudes of community-dwelling older adults in Singapore (2) examine the differences between their nutrition knowledge, and socio-demographic factors, competencies and attitudes, and (3) identify factors associated with better nutrition knowledge in community-dwelling older adults in Singapore. Methods 400 (183 males and 217 females) nourished community-dwelling older adults aged 65 years and above took part in this study. Malnutrition Universal Screening Tool (MUST) was used to determine individuals who were at low risk of undernutrition. Nutrition knowledge, competencies, attitudes and sources of nutrition information were measured using a locally developed scale. Nutrition knowledge scores were summed to form the Nutrition Knowledge Index (NKI). Associations between NKI, competencies, attitudes and socio-demographic variables were examined using Chi-square and Fisher’s Exact tests. Factors associated with NKI were determined using a stepwise regression model with resampling based methods for model averaging. Results Bivariate analyses found significant differences in NKI scores for gender, monthly household earnings, type of housing, the self-reported ability to seek and understand nutrition information and having access to help from family/friends. Females had higher NKI scores compared to males ( p < 0.001). Compared to females, more males left food decisions to others ( p < 0.001), and fewer males reported consuming home cooked food ( p = 0.016). Differences in educational level were found for competencies like the self-reported ability to seek ( p < 0.001) and verify nutrition information ( p < 0.001). Stepwise regression analysis showed that being female, Chinese, self-reported ability to understand nutrition information and having access to help from family/friends were associated with higher NKI scores. Conclusions Our study revealed that nutrition knowledge of older males in Singapore was lower than females and more left food decisions to others. Nutrition education programs could be targeted at both the older male and their caregivers. Trial Registration This study was registered on 7 August 2017 at clinicaltrials.gov (ref. NCT03240952).
Positive association between physical outcomes and patient-reported outcomes in late-onset Pompe disease: a cross sectional study
Authors: Yuan Meng , Andrinopoulou Eleni-Rosalina , Kruijshaar Michelle E. , Lika Aglina , Harlaar Laurike , Ploeg Ans T Van Der , Rizopoulos Dimitris , Beek Nadine A.M.E. van der 
Published: May 2020

Abstract
Background Pompe disease is a rare, progressive metabolic myopathy. The aim of this study is to investigate the associations of physical outcomes with patient-reported outcome measures (PROMs) in late-onset Pompe disease. Methods We included 121 Dutch adult patients with Pompe disease. Physical outcomes comprised muscle strength (manual muscle testing using Medical Research Council [MRC] grading, hand-held dynamometry [HHD]), walking ability (6-minute walk test [6MWT]), and pulmonary function (forced vital capacity [FVC] in upright and supine positions). PROMs comprised quality of life (Short Form 36 health survey [SF-36]), participation (Rotterdam Handicap Scale [RHS]) and daily-life activities (Rasch-Built Pompe-Specific Activity [R-PAct] Scale). Analyses were cross-sectional: the time-point before, and closest to, start of Enzyme Replacement Therapy was chosen. Associations between PROMs and physical outcomes were investigated using linear regression models. Results RHS and R-PAct scores were better in patients with higher FVC supine and upright, HHD, MRC and 6MWT scores, accounting for the effect of sex, disease duration, use of wheelchair and ventilator support. While the SF-36 Physical Component Summary (PCS) was correlated positively with FVC upright, HHD, MRC and 6MWT scores, there was no significant relationship between the SF-36 Mental Component Summary (MCS) and any of the physical outcomes. Conclusions Participation, daily-life activities, and the physical component of quality of life of adult Pompe patients are positively correlated to physical outcomes. This work serves as a first step towards assessing how changes over time in physical outcomes are related to changes in PROMs, and to define the minimal change in physical outcomes required to make an important difference for the patient.
Two novel heterozygote mutations of ATM in a Chinese family with dystonia-dominant ataxia telangiectasia and literatures review
Authors: Liu Zhi-Jun , Wang Ya-Ling , xu yan 
Published: May 2020

Abstract
Background Ataxia-telangiectasia (A-T) is an autosomal recessive disorder with high clinical heterogeneity. A-T may present in complicated variable forms, mainly including classic A-T and milder forms. Contrary to the classic A-T, the milder form does not present the cardinal features of A-T, including ataxia and telangiectasia. A few ATM mutations have been reported in variant A-T cases manifested as isolated dystonia without any signs of classical A-T. To date, more than 400 disease-related ATM mutations have been identified in patients with A-T. Methods A pedigree with A-T and predominant dystonia were collected. Genetic testing of the proband were performed by target exome-sequencing of a panel designed to cover 101 genes associated with movement disorders. The candidate variants were further confirmed by Sanger sequencing.Results Two novel ATM variants (p.I2683T and p.S2860P) with probable pathogenicity were identified in the family. The proband presented obviously isolated segmental dystonia without any signs of ataxia and telangiectasias. We then reviewed previously published literatures of genetically confirmed A-T cases with predominant dystonia and summarized the clinical characteristics of dystonia-dominant A-T. Conclusion This is the first report of A-T patient with predominant dystonia in China. Dystonia may appear as one of the predominant manifestations or initial symptom of A-T. ATM genetic testing should be early considered for those patients with predominant dystonia, despite without accompanying ataxia or telangiectasia.
Caspase-3 Silencing Attenuates Tumor Repopulation Via Impairing Radiation-Induced DNA Damage Response and Downstream Pathway in Non-Small-Cell Lung Cancer
Authors: Zhao Minghui , Wang Yiwei , Zhao Yucui , He Sijia , Zhao Ruyi , Song Yanwei , Cheng Jin , Gong Yanping , Xie Jianzhu , Wang Yulan , Hu Binjie , Tian Ling , Huang Qian 
Published: May 2020

Abstract
Background: Radiotherapy is an effective treatment on non-small-cell lung cancer (NSCLC). However, radiation-induced dying tumor cells are postulated to generate potent growth signals to stimulate the repopulation of adjacent surviving tumor cells, which may promote tumor recurrence. We investigated the role of caspase-3-centered molecular mechanism in NSCLC repopulation after radiotherapy.Methods: The stable caspase-3 knockout (Casp3 KO) NSCLC cells were compared with wild-type cells for growth-promoting effect after radiotherapy using in vitro repopulation model. Western blotting, quantitative real-time PCR, enzyme-linked immunosorbent assay and luciferase reporter assay were used to identify the possible molecules and pathway. To elucidate the function of caspase-3 in tumor repopulation, a series of in vitro assays were performed with Casp3 KO NSCLC cells. Finally, tumor cell growth rate of Casp3 KO and wild-type tumor cells in vivo was tested using xenograft tumor assay and key proteins were further confirmed in tumor tissues with or without radiotherapy.Results: We found that radiation induced DNA damage response (DDR) and caspase-3 activation, as well as promoted tumor repopulation in NSCLC cells. Unexpectedly, depleting caspase-3 significantly attenuated ataxia-telangiectasia mutated kinase (ATM)/p53-mediated DDR via attenuating endonuclease G (EndoG) nuclear migration, thus decreasing the growth-promoting effect of irradiated dying cells. Moreover, we identified p53 as a regulator of the Cox-2/PGE2 axis, which was probably involved in caspase-3-centered tumor repopulation after radiotherapy. Additionally, depleting caspase-3 in NSCLC cells showed impaired tumor growth in nude mice model.Conclusions: Our findings demonstrated that caspase-3 was implicated in tumor repopulation and that was accompanied by promoting DDR and downstream Cox-2/PGE2 axis activation in NSCLC cells. This hitherto undescribed signaling pathway mediated by caspase-3 may deepen insight into the radiobiology and provide therapeutic targets to reduce NSCLC recurrence after radiotherapy.
IFN-γ Enhances the Therapeutic Effect of Mscs on Experimental Renal Fibrosis
Authors: Kanai Ryo , Nakashima Ayumu , Doi Shigehiro , Kimura Tomoe , Yoshida Ken , Maeda Satoshi , Ishiuchi Naoki , Yamada Yumi , Ike Takeshi , Doi Toshiki , Kato Yukio , Masaki Takao 
Published: May 2020

Abstract
Background:Mesenchymal stem cells (MSCs) administered for therapeutic purposes can be activated by interferon-γ (IFN-γ) secreted from natural killer cells in injured tissues and exert anti-inflammatory effects. These processes may require a substantial period of time, leading to a delayed onset of MSCs therapeutic effects. Here, we investigatedwhetherpretreatment with IFN-γ potentiates the anti-fibrotic and anti-inflammatory activities of MSCs in an injured kidney. Methods:MSCs with or without IFN-γ treatment were injected into rats after ischemia-reperfusion injury (IRI) or unilateral ureter obstruction (UUO) to evaluate the therapeutic effect of IFN-γ-treated MSCs. In addition, we used conditioned medium obtained from IFN-γ-treated MSCs to investigate fibrotic change in cultured cells induced by transforming growth factor-β1 and phenotypic change of macrophages using THP-1 monocytes.Results:Administration of MSCs treated with IFN-γ strongly ameliorated renal fibrosis and inflammation in rat IRI and UUO models compared with that of untreated MSCs. In vitro experiments demonstrated that IFN-γ treatment enhanced the anti-fibrotic effects of MSCs by inhibiting the transforming growth factor-β1/Smad signaling pathway. Most notably, secretion of prostaglandin E2 from MSCs was significantly increased by treatment with IFN-γ. Increased prostaglandin E2 induced polarization of immunosuppressive CD163-positive macrophages. In addition, knockdown of prostaglandin E synthase weakened the anti-fibrotic effects of MSCs treated with IFN-γ in IRI rats, suggesting the involvement of prostaglandin E2 in the beneficial effects of IFN-γ.Conclusions:Administration of MSCs treated with IFN-γ may represent a promising therapy to prevent the progression of renal fibrosis.
A strain affiliated to Bacillus amyloliquefaciens alleviates high-carbohydrate diet- induced metabolic syndrome by restoration of acetate-producing bacteria in fish intestines
Authors: Xu Rong , Li Miao , Wang Tong , Zhao Yi-Wei , Shan Cheng-Jie , Qiao Fang , Chen Li-Qiao , Du Zhen-Yu , Zhang Mei-Ling 
Published: May 2020

Abstract
Background: Increasing the utilization efficiency of high-carbohydrate diet has the potential to promote “protein sparing effects” in farmed fish; however, many fish utilize carbohydrates poorly. The intestinal microbiota plays an important role in carbohydrate degradation. Whether the addition of functional bacteria could increase the carbohydrate utilization efficiency and alleviate high-carbohydrate diet-induced adverse effects is unknown.Results: A bacterial strain that could degrade starch in vitro was isolated from the intestines of Nile tilapia (Oreochromis niloticus). The bacterium was affiliated to Bacillus amyloliquefaciens (designated as B. amy SS1) based on 16S rRNA gene sequencing. Three diets, including control diet (CON), high-carbohydrate diet (HCD), and high-carbohydrate diet supplemented with B. amy SS1 (HCB), were used to feed Nile tilapia for 10 weeks. The beneficial effects of B. amy SS1 on weight gain and protein accumulation were observed. The HCB decreased blood glucose levels and reduced lipid deposition compared with the HCD group. To detect the possible mechanism, the intestinal microbiota composition was characterized using high-throughput sequencing. The HCB increased the abundance of short-chain fatty acid-producing bacteria. Gas chromatographic analysis indicated that theconcentration of acetate increased dramatically in the HCB group compared with that in the HCD group. Glucagon-like peptide-1 (GLP-1) levels increased in the intestine and serum of the HCB group. Different concentrations of sodium acetate (low (HLA), 900 mg/kg; medium (HMA), 1800 mg/kg, and high (HHA), 3600 mg/kg) were added to the HCD to feed the fish for eight weeks. The HMA and HHA groups mirrored the effects of the HCD supplemented with B. amy SS1 by increasing serum GLP-1 levels. Increased acetate concentrations stimulated GLP-1 production, which might account for the effects caused by the addition of B. amy SS1 to the HCD.Conclusions: This study systematically analyzed the influence of B. amy SS1 on fish metabolism, suggesting that B. amy SS1 treatment alleviates the metabolic syndrome caused by HCD by enriching acetate-producing bacteria in fish intestines. Regulating the intestinal microbiota and their metabolites might represent a powerful strategy for fish nutrition modulation and health maintenance in future.