bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mobile primary health care clinics for Indigenous populations in Australia, Canada, New Zealand and the United States: a systematic scoping review
Authors: Beks Hannah , Ewing Geraldine , Charles James A. , Mitchell Fiona , Paradies Yin , Clark Robyn A. , Versace Vincent L. 
Published: November 2020

Abstract

Background
Mobile clinics have been used to deliver primary health care to populations that otherwise experience difficulty in accessing services. Indigenous populations in Australia, Canada, New Zealand, and the United States experience greater health inequities than non-Indigenous populations. There is increasing support for Indigenous-governed and culturally accessible primary health care services which meet the needs of Indigenous populations. There is some support for primary health care mobile clinics implemented specifically for Indigenous populations to improve health service accessibility. The purpose of this review is to scope the literature for evidence of mobile primary health care clinics implemented specifically for Indigenous populations in Australia, Canada, New Zealand, and the United States.

Methods
This review was undertaken using the Joanna Brigg Institute (JBI) scoping review methodology. Review objectives, inclusion criteria and methods were specified in advance and documented in a published protocol. The search included five academic databases and an extensive search of the grey literature.

Results
The search resulted in 1350 unique citations, with 91 of these citations retrieved from the grey literature and targeted organisational websites. Title, abstract and full-text screening was conducted independently by two reviewers, with 123 citations undergoing full text review. Of these, 39 citations discussing 25 mobile clinics, met the inclusion criteria. An additional 14 citations were snowballed from a review of the reference lists of included citations. Of these 25 mobile clinics, the majority were implemented in Australia (n = 14), followed by United States (n = 6) and Canada (n = 5). No primary health mobile clinics specifically for Indigenous people in New Zealand were retrieved. There was a pattern of declining locations serviced by mobile clinics with an increasing population. Furthermore, only 13 mobile clinics had some form of evaluation.

Conclusions
This review identifies geographical gaps in the implementation of primary health care mobile clinics for Indigenous populations in Australia, Canada, New Zealand, and the United States. There is a paucity of evaluations supporting the use of mobile clinics for Indigenous populations and a need for organisations implementing mobile clinics specifically for Indigenous populations to share their experiences. Engaging with the perspectives of Indigenous people accessing mobile clinic services is imperative to future evaluations.

Registration
The protocol for this review has been peer-reviewed and published in JBI Evidence Synthesis (doi: 10.11124/JBISRIR-D-19-00057).
SVFX: a machine learning framework to quantify the pathogenicity of structural variants
Authors: Kumar Sushant , Harmanci Arif , Vytheeswaran Jagath , Gerstein Mark B. 
Published: November 2020

Abstract
There is a lack of approaches for identifying pathogenic genomic structural variants (SVs) although they play a crucial role in many diseases. We present a mechanism-agnostic machine learning-based workflow, called SVFX, to assign pathogenicity scores to somatic and germline SVs. In particular, we generate somatic and germline training models, which include genomic, epigenomic, and conservation-based features, for SV call sets in diseased and healthy individuals. We then apply SVFX to SVs in cancer and other diseases; SVFX achieves high accuracy in identifying pathogenic SVs. Predicted pathogenic SVs in cancer cohorts are enriched among known cancer genes and many cancer-related pathways.
Microbial community analysis with a specific statistical approach after a record breaking snowfall in Southern Italy
Authors: Monaco Pamela , Divino Fabio , Naclerio Gino , Bucci Antonio 
Published: November 2020

AbstractPurposeSnow and ice ecosystems present unexpectedly high microbial abundance and diversity. Although arctic and alpine snow environments have been intensively investigated from a microbiological point of view, few studies have been conducted in the Apennines. Accordingly, the main purpose of this research was to analyze the microbial communities of the snow collected in two different locations of Capracotta municipality (Southern Italy) after a snowfall record occurred on March 2015 (256 cm of snow in less than 24 h).MethodsBacterial communities were analyzed by the Next-Generation Sequencing techniques. Furthermore, a specific statistical approach for taxonomic hierarchy data was introduced, both for the assessment of diversity within microbial communities and the comparison between different microbiotas. In general, diversity and similarity indices are more informative when computed at the lowest level of the taxonomic hierarchy, the species level. This is not the case with microbial data, for which the species level is not necessarily the most informative. Indeed, the possibility to detect a large number of unclassified records at every level of the hierarchy (even at the top) is very realistic due to both the partial knowledge about the cultivable fraction of microbial communities and limitations to taxonomic assignment connected to the quality and completeness of the 16S rRNA gene reference databases. Thus, a global approach considering information from the whole taxonomic hierarchy was adopted in order to obtain a more consistent assessment of the biodiversity.ResultThe main phyla retrieved in the investigated snow samples wereProteobacteria,Actinobacteria,Bacteroidetes, andFirmicutes. Interestingly, DNA from bacteria adapted to thrive at low temperatures, but also from microorganisms normally associated with other habitats, whose presence in the snow could be justified by wind-transport, was found. Biomolecular investigations and statistical data analysis showed relevant differences in terms of biodiversity, composition, and distribution of bacterial species between the studied snow samples.ConclusionThe relevance of this research lies in the expansion of knowledge about microorganisms associated with cold environments in contexts poorly investigated such as the Italian Apennines, and in the development of a global statistical approach for the assessment of biological diversity and similarity of microbial communities as an additional tool to be usefully combined with the barcoding methods.
Anti-inflammatory effects of 18-nor-ent-pimara-9(11),15-diene-4β-ol isolated from the roots of Aralia continentalis on LPS-induced in RAW264.7 cells
Authors: Eo Hyun Ji , Park Youngki , Hong Seong Su , Park Gwang Hun 
Published: November 2020

Abstract
Aralia continentalis (A. continentalis) is a medicinal plant belonging to Araliaceae, it has been reported to exert anti-cancer, anti-bacterial, anti-inflammatory, anti-platelet and anti-oxidative activities. But the potential mechanism for the anti-inflammatory effect of compounds isolated from the roots of A. continentalis is still insufficient. So, we evaluated whether compounds isolated from the roots of A. continentalis exert anti-inflammatory effects and elucidated its potential mechanism in RAW264.7 cells. The concentrated residue was subsequently suspended in H2O and partitioned with n-hexane, methylene chloride (CH2Cl2), ethyl acetate (EtOAc) and n-butanol (n-BuOH). The fractions were subjected to sequential column chromatography over silica-gel, RP-18, MPLC, recycling and preparative HPLC to isolated the novel compound. The novel compound was identified as 18-nor-ent-pimara-9(11),15-diene-4β-ol and confirmed anti-inflammatory activity. The 18-nor-ent-pimara-9(11),15-diene-4β-ol dose-dependently blocked NO production and inhibited iNOS, COX-2, TNF-α and IL-1β expression in LPS-stimulated RAW264.7 cells. The 18-nor-ent-pimara-9(11),15-diene-4β-ol inhibited LPS-stimulated degradation of IκB-α and nuclear accumulation of p65, which resulted in the suppression of NF-κB activation in RAW264.7 cells. Also, the 18-nor-ent-pimara-9(11),15-diene-4β-ol attenuated the phosphorylation of p38 and ERK1/2 in LPS-induced RAW264.7 cells. These results suggest that the nor-ent-pimara-9(11),15-diene-4β-ol isolated from the roots of A. continentalis may have grate potential for the development of anti-inflammatory drugs.
In situ virtual restoration of artifacts by imaging technology
Authors: Qiao Chengquan , Zhang Wenwen , Gong Decai , Gong Yuxuan 
Published: November 2020

Abstract
The restoration of incomplete artifacts is important for presenting the value of artifacts. Even though the existing methods provide rich solutions for the restoration of artifacts, they may not be the ultimate and perfect methods. The innovation of restoration methods may provide better restoration effects in some aspects or bring people a different visiting experience, which motivates people to explore new restoration methods. In this paper, based on virtual imaging technology, a virtual imaging restoration method for incomplete artifacts were proposed. In order to examine the effects and potential of the virtual imaging restoration, a bronze mirror and a mural were restored through our newly invented virtual imaging restoration device. The effects of the restorations were evaluated through the illumination test and the questionnaire surveys. The results proved that the virtual imaging restoration can visually achieve in situ restoration, and it is completely reversible. Virtual imaging restoration also performed well in terms of visiting experience, authenticity, discernibility, and economy. Limitations of this restoration method are related to the scope of application, the viewing angle, and the imaging effects. Overall, virtual imaging restoration is worth recommending and the potential of this method and its encouraging results may provide a choice or pretest tool for the restoration of some incomplete artifacts in the near future.
Large overjet as a risk factor of traumatic dental injuries: a prospective longitudinal study
Authors: Schatz Jean-Paul , Ostini Enrico , Hakeberg Magnus , Kiliaridis Stavros 
Published: November 2020

Abstract

Purpose
The aim of this longitudinal prospective study was to evaluate if schoolchildren with large overjet experience a greater risk of traumatic dental injuries (TDI) compared to children with normal or small overjet.

Methods
A sample of children aged 6‑13 years was prospectively evaluated after 1 year: from the initial sample, data concerning trauma cases of 1413 children were collected to determine the number and types of injuries, the influence of overjet on the risk of TDI, and the relationships between trauma, age, and gender.

Results
The observed prevalence of trauma was higher for boys, with the largest frequency between the ages of 8 to 12 years: 67.9% of all injuries were hard tissue injuries and 32.1% subluxation and luxation injuries. Children with an overjet of 6 mm or more showed a statistically increased risk of getting trauma [RR = 3.37 with CI (1.81; 6.27)].

Conclusion
In this prospective study, overjet stood out among variables as the most significant risk factor of TDI: an increased overjet of 6 mm or more had a major impact on the risk of trauma, which would speak in favor of early orthodontic correction of an increased overjet to reduce the prevalence of dental trauma
Protective effects of peel extracts of Irvingia wombolu on metabolic disorders in streptozotocin-induced diabetic rats
Authors: Matsinkou Rosane Soh , Oumbe Valere Aime Soh , Ngondi Judith Laure , Oben Julius Enyong 
Published: November 2020

AbstractBackgroundDiabetes is widely recognized as associated with several structural and functional liver, kidney, and heart abnormalities. Therefore, the present study was conducted to evaluate the protective effect of peel extracts ofIrvingia womboluagainst diabetes complications.MethodsDiabetes was induced by intravenous administration of streptozotocin (STZ) (50 mg/kg) through the right jugular vein on rats and animals with blood glucose values of at least 250 mg/dl received orally aqueous extract of peel (AEP), hydroethanolic extract of peel (HEP), tolbutamide and DMSO 10%. Their effects on the concentration of blood glucose, total cholesterol, HDL-C, LDL-C, triglycerides, malondialdehyde (MDA) and activities of catalase (CAT), reduced glutathione (GSH), superoxide dismutase (SOD), aspartate aminotransferase (AST), and alanine aminotransferase (ALT) in diabetic and normal rats were investigated using standard methods.ResultsTwenty-one days of treatment with AEP and HEP at a dose of 400 mg/kg significantly (P < 0,05) reduced the fasting blood glucose to a point of reaching normal value. The antihyperlipidemic assessment of extracts revealed a significant (P < 0,05) decrease in total cholesterol, triglycerides, LDL levels, and a significant (P < 0,05) increase in HDL level in the plasma of treated diabetic rats. Furthermore, plasma biomarkers of liver and kidney dysfunction were significantly reduced in treated diabetic rats. We also observed increased activities of catalase, SOD, and reduced glutathione in diabetic treated rats.ConclusionThe present findings suggest that AEP and HEP have a protective effect on liver, kidney, and heart in experimental diabetic rats which can be beneficial in the management of diabetes and its complications.
Automated synthesis of 18F radiolabelled indole containing Oncrasin-like molecules; a comparison of iodonium salts and boronic ester chemistry
Authors: McDonald Alexander F. , Goh Yit Wooi , White Jonathan M. , Scott Andrew M. , Ackermann Uwe 
Published: November 2020

Abstract

Background
Oncrasin-1 is a small molecule which was identified from a screen of KRAS mutant cancer cells and has shown specificity for KRAS mutant cell killing. We aimed to develop a radiolabelled form of Oncrasin-1 to enable in-vivo imaging of mutant KRAS expression in malignant tumours. This work outlines the synthesis of 3 fluorinated derivatives and development of iodonium salt and boronic ester precursors for radiolabelling with the 18F isotope.

Results
In our hands, synthesis of iodonium salts were not easily accessible due to the 3-carbaldehyde indole structure being preferentially oxidized by conditions required for iodonium salt formation, rather than benzyl iodide. Synthesis and radiolabelling of boronic acid pinacol ester precursors were successful, with the products being obtained in yields of 10.76% ± 0.96% (n = 5), 14.7% ±8.58% (n = 3) and 14.92% ±3.9% (n = 3) for 18F KAM001, 18F KAM002 and 18F KAM003 respectively, with radiochemical purity of greater than 99%.

Conclusions
The successful synthesis of these tracers has been undertaken utilizing boronic ester radio-fluorination methods and will allow for investigation of Oncrasin based molecules as potential diagnostics for cancers expressing mutant KRAS protein.
Negotiating growth of online education in higher education
Authors: Morris Neil P. , Ivancheva Mariya , Coop Taryn , Mogliacci Rada , Swinnerton Bronwen 
Published: November 2020

Abstract
Universities are facing growing internal and external pressures to generate income, educate a widening continuum of learners, and make effective use of digital technologies. One response has been growth of online education, catalysed by Massive Open Online Courses, availability of digital devices and technologies, and notions of borderless global education. In growing online education, learning and teaching provision has become increasingly disaggregated, and universities are partnering with a range of private companies to reach new learners, and commercialise educational provision. In this paper, we explore the competing drivers which impact decision making within English universities and their strategies to grow online education provision, through interviews with senior managers, and interrogation of their views through the lens of a range of internal, external and organisational drivers. We show that pressures facing universities may be alleviated by growth of online education provision, but that negotiating an appropriate route to realise this ambition involves attempts to resolve these underlying tensions deriving from competing drivers. We use a modified form of the PEST model to demonstrate the complexities, inter-dependencies and processes associated with these drivers when negotiating delivery of unbundled online education through use of private company services, or in partnership with private companies.
Validity and reliability testing of the Toddler and Infant (TANDI) Health Related Quality of Life instrument for very young children
Authors: Verstraete Janine , Ramma Lebogang , Jelsma Jennifer 
Published: November 2020

AbstractBackgroundDespite the high burden of disease in younger children there are few tools specifically designed to estimate Health Related Quality of Life (HRQoL) in children younger than 3 years of age. A previous paper described the process of identifying a pool of items which might be suitable for measuring HRQoL of children aged 0–3 years. The current paper describes how the items were pruned and the final draft of the measure, Toddler and Infant (TANDI) Health Related Quality of Life, was tested for validity and reliability.MethodsA sample of 187 caregivers of children 1–36 months of age were recruited which included children who were either acutely ill (AI), chronically ill (CI) or from the general school going population (GP). The TANDI, an experimental version of the EQ-5D-Y proxy, included six dimensions with three levels of report and general health measured on a Visual Analogue Scale (VAS) from 0 to 100. The content validity had been established during the development of the instrument. The TANDI, Ages and Stages Questionnaire (ASQ), Faces, Leg, Activity, Cry, Consolabilty (FLACC) or Neonatal Infant Pain Scale (NIPS) and a self-designed dietary information questionnaire were administered at baseline. The TANDI was administered 1 week later in GP children to establish test-retest reliability. The distribution of dimension scores, Cronbach’s alpha, rotated varimax factor analysis, Spearman’s Rho Correlation, the intraclass correlation coefficient, Pearson’s correlation, analysis of variance and regression analysis were used to explore the reliability, and validity of the TANDI.ResultsConcurrent validity of the different dimensions was tested between the TANDI and other instruments. The Spearman’s Rho coefficients were significant and moderate to strong for dimensions of activity and participation and significant and weak for items of body functions. Known groups were compared and children with acute illness had the lowest ranked VAS (median 60, range 0–100), indicating worse HRQoL. The six dimensions of the TANDI were tested for internal consistency and reliability and the Cronbach’s α as 0.83. Test-retest results showed no variance for dimension scores of movement and play, and high agreement for pain (83%), relationships (87%), communication (83%) and eating (74%). The scores were highly correlated for the VAS (ICC = 0.76;p < 0.001).ConclusionThe TANDI was found to be valid and reliable for use with children aged 1–36 months in South Africa. It is recommended that the TANDI be included in future research to further investigate HRQoL and the impact of interventions in this vulnerable age group. It is further recommended that future testing be done to assess the feasibility, clinical utility, and cross-cultural validity of the measure and to include international input in further development.
An insight into artisanal leather making in Ghana
Authors: Appiah-Brempong Miriam , Essandoh Helen M. K. , Asiedu Nana Yaw , Dadzie Samuel Kwame , Momade Francis Warings Yao 
Published: November 2020

Abstract
The production of leather from animal skins and hides through tanning processes began in the pre-historic ages. Despite the origination of new leather making techniques such as chrome tanning which is being employed extensively today, the traditional method of leather production primarily through vegetable tanning is still being practised mostly in artisanal tanneries in developing nations including Ghana. Artisanal leather making, thus, contributes beneficially to rural livelihood. Nevertheless, the growth of this sector has been stifled by the lack of innovative technologies to enhance productivity. This challenge could be partly linked to the knowledge gap on the scientific principles governing artisanal leather production processes. This study, therefore, elaborates on various process steps and materials employed in traditional leather making in Ghana and the scientific principles underlying each of the processes. It also makes a comparison between traditional and modern leather manufacturing processes and identifies knowledge and technological gaps which would inspire in-depth scientific research into artisanal leather making.

Graphical abstract
Genes predisposing to syndromic and nonsyndromic infertility: a narrative review
Authors: Yahaya Tajudeen O. , Liman Usman U. , Abdullahi Haliru , Koko Yahuza S. , Ribah Samuel S. , Adamu Zulkarnain , Abubakar Suleiman 
Published: November 2020

AbstractBackgroundAdvanced biological techniques have helped produce more insightful findings on the genetic etiology of infertility that may lead to better management of the condition. This review provides an update on genes predisposing to syndromic and nonsyndromic infertility.Main bodyThe review identified 65 genes linked with infertility and infertility-related disorders. These genes regulate fertility. However, mutational loss of the functions of the genes predisposes to infertility. Twenty-three (23) genes representing 35% were linked with syndromic infertility, while 42 genes (65%) cause nonsyndromic infertility. Of the 42 nonsyndromic genes, 26 predispose to spermatogenic failure and sperm morphological abnormalities, 11 cause ovarian failures, and 5 cause sex reversal and puberty delay. Overall, 31 genes (48%) predispose to male infertility, 15 genes (23%) cause female infertility, and 19 genes (29%) predispose to both. The common feature of male infertility was spermatogenic failure and sperm morphology abnormalities, while ovarian failure has been the most frequently reported among infertile females. The mechanisms leading to these pathologies are gene-specific, which, if targeted in the affected, may lead to improved treatment.ConclusionsMutational loss of the functions of some genes involved in the development and maintenance of fertility may predispose to syndromic or nonsyndromic infertility via gene-specific mechanisms. A treatment procedure that targets the affected gene(s) in individuals expressing infertility may lead to improved treatment.