bluuebunny/crossref_metadata_2025_split huggingface dataset:
English medium instruction: teachers’ challenges and coping strategies
Authors: Pun Jack K H , Thomas Nathan 
Published: April 2020

Abstract
English medium instruction (EMI) is a growing phenomenon in many countries. Some researchers have investigated the difficulties and strategies students use to cope with EMI. However, the pedagogical challenges faced and strategies used by other key participants, teachers, have been surprisingly under-researched. This study utilizes questionnaire and interview data to identify the challenges and coping strategies used by secondary school EMI science teachers in Hong Kong. A number of challenges and strategies were reported. The authors argue that many of these challenges can be overcome with principles and strategies from ELT, as the ‘English’ in EMI is often the main issue. The most prevalent strategy reported is the use of L1. Two perspectives on L1 usage in EMI contexts are discussed. Two emergent realities are presented as possible solutions: the idealized reality and the immediate reality. Implications for teachers and suggestions for future research are provided.
Priorities for improving chemicals management in the WHO European Region—stakeholders’ views
Authors: Vincenten Joanne A , Zastenskaya Irina , Schröder-Bäck Peter , Jarosinska Dorota Iwona 
Published: April 2020

Abstract

Background
Prevention of the impact of chemicals on human health and the environment is an increasing focus of public health polices and policy makers. The World Health Organization European Centre for Environment and Health wanted to know what were stakeholders’ priorities for improving chemicals management and prevention.

Methods
Semi-structured interviews were undertaken with 18 diverse stakeholders to answer this question. The interview questionnaire was developed using current WHO chemical meeting reports, the Evidence Implementation Model for Public Health Systems and categories of the theory of diffusion. Stakeholder views were attained on three main questions within the questionnaire. (i) What priority actions should be undertaken to minimize the negative impact of chemicals? (ii) Who needs to be more involved and what roles should they have? (iii) How can science and knowledge on chemicals and health be translated into policies more effectively and what are the greatest barriers to overcome?

Results
Cross cutting issues, such as legislation strengthening and enforcement, further collection of information, capacity building, education and awareness raising were considered priorities. The responders had the same vision on roles and responsibilities of different stakeholders. The greatest barrier to adoption, implementation and enforcement of evidence-based policies reported was leadership and political commitment to chemical safety.

Conclusions
Priorities raised differed depending on knowledge, professional background and type of stakeholder. Factors influencing priority identification at the national level include international and global context, availability of information, knowledge of the current situation and evidence-based good practice, and risks and priorities identified through national assessments.
Isolation and characterization of the novel oil-degrading strain Kosakonia cowanii IUMR B67 and expression of the degradation enzyme
Authors: Ren Jianjun , Wang Zhenzhu , Niu Dongze , Huhetaoli  , Huang Xu , Fan Bo , Li Chunyu 
Published: April 2020

Abstract
Oils in food waste can pollute the environment and negatively affect human health. Biodegradation is a promising method for disposing of waste edible oils. In this study, an oil-degrading bacterium was isolated from kitchen waste for efficient degradation of edible oils. Its growth and oil degradation characteristics were investigated in basic salt medium with edible oils as the sole carbon and energy source; the triacylglycerol lipase gene (EC 3.1.1.3) was cloned and expressed in Escherichia coli. A novel oil-degrading bacterium assigned as IUMR B67 was successfully isolated. Morphological and molecular analyses revealed that strain IUMR B67 belongs to Kosakonia cowanii. After 144 h of incubation, the oil degradation rate at 37°C was 95.80%. Optimal conditions for IUMR B67 were recorded at 37°C and 0.1% NaCl with 0.1% ammonium sulfate supplementation. The lipase gene of strain IUMR B67 was determined to be 912 base pairs, and the lipase activity of the expressed protein was 3.02 U/mL, which was significantly higher than the control (P &lt; 0.05). Overall, Kosakonia cowanii IUMR B67 is a novel edible oil-degrading strain that can hydrolyze oil via its lipase activity, which may be useful in the disposal of oils and oily food waste.
The Epithelial adhesin 1 tandem repeat region mediates protein display through multiple mechanisms
Authors: Raposo Colin J , McElroy Kyle A , Fuchs Stephen M 
Published: April 2020

ABSTRACT
The pathogenic yeast Candida glabrata is reliant on a suite of cell surface adhesins that play a variety of roles necessary for transmission, establishment and proliferation during infection. One particular adhesin, Epithelial Adhesin 1 [Epa1p], is responsible for binding to host tissue, a process which is essential for fungal propagation. Epa1p structure consists of three domains: an N-terminal intercellular binding domain responsible for epithelial cell binding, a C-terminal GPI anchor for cell wall linkage and a serine/threonine-rich linker domain connecting these terminal domains. The linker domain contains a 40-amino acid tandem repeat region, which we have found to be variable in repeat copy number between isolates from clinical sources. We hypothesized that natural variation in Epa1p repeat copy may modulate protein function. To test this, we recombinantly expressed Epa1p with various repeat copy numbers in S. cerevisiae to determine how differences in repeat copy number affect Epa1p expression, surface display and binding to human epithelial cells. Our data suggest that repeat copy number variation has pleiotropic effects, influencing gene expression, protein surface display and shedding from the cell surface of the Epa1p adhesin. This study serves to demonstrate repeat copy number variation can modulate protein function through a number of mechanisms in order to contribute to pathogenicity of C. glabrata.
ALS-associated genes in SCA2 mouse spinal cord transcriptomes
Authors: Scoles Daniel R , Dansithong Warunee , Pflieger Lance T , Paul Sharan , Gandelman Mandi , Figueroa Karla P , Rigo Frank , Bennett C Frank , Pulst Stefan M 
Published: April 2020

Abstract
The spinocerebellar ataxia type 2 (SCA2) gene ATXN2 has a prominent role in the pathogenesis and treatment of amyotrophic lateral sclerosis (ALS). In addition to cerebellar ataxia, motor neuron disease is often seen in SCA2, and ATXN2 CAG repeat expansions in the long normal range increase ALS risk. Also, lowering ATXN2 expression in TDP-43 ALS mice prolongs their survival. Here we investigated the ATXN2 relationship with motor neuron dysfunction in vivo by comparing spinal cord (SC) transcriptomes reported from TDP-43 and SOD1 ALS mice and ALS patients with those from SCA2 mice. SC transcriptomes were determined using an SCA2 bacterial artificial chromosome mouse model expressing polyglutamine expanded ATXN2. SCA2 cerebellar transcriptomes were also determined, and we also investigated the modification of gene expression following treatment of SCA2 mice with an antisense oligonucleotide (ASO) lowering ATXN2 expression. Differentially expressed genes (DEGs) defined three interconnected pathways (innate immunity, fatty acid biosynthesis and cholesterol biosynthesis) in separate modules identified by weighted gene co-expression network analysis. Other key pathways included the complement system and lysosome/phagosome pathways. Of all DEGs in SC, 12.6% were also dysregulated in the cerebellum. Treatment of mice with an ATXN2 ASO also modified innate immunity, the complement system and lysosome/phagosome pathways. This study provides new insights into the underlying molecular basis of SCA2 SC phenotypes and demonstrates annotated pathways shared with TDP-43 and SOD1 ALS mice and ALS patients. It also emphasizes the importance of ATXN2 in motor neuron degeneration and confirms ATXN2 as a therapeutic target.
Loss of function mutations in CCDC32 cause a congenital syndrome characterized by craniofacial, cardiac and neurodevelopmental anomalies
Authors: Harel Tamar , Griffin John N , Arbogast Thomas , Monroe Tanner O , Palombo Flavia , Martinelli Marcella , Seri Marco , Pippucci Tommaso , Elpeleg Orly , Katsanis Nicholas 
Published: April 2020

Abstract
Despite the wide use of genomics to investigate the molecular basis of rare congenital malformations, a significant fraction of patients remains bereft of diagnosis. As part of our continuous effort to recruit and perform genomic and functional studies on such cohorts, we investigated the genetic and mechanistic cause of disease in two independent consanguineous families affected by overlapping craniofacial, cardiac, laterality and neurodevelopmental anomalies. Using whole exome sequencing, we identified homozygous frameshift CCDC32 variants in three affected individuals. Functional analysis in a zebrafish model revealed that ccdc32 depletion recapitulates the human phenotypes. Because some of the patient phenotypes overlap defects common to ciliopathies, we asked if loss of CCDC32 might contribute to the dysfunction of this organelle. Consistent with this hypothesis, we show that ccdc32 is required for normal cilia formation in zebrafish embryos and mammalian cell culture, arguing that ciliary defects are at least partially involved in the pathomechanism of this disorder.
Surgical repair of a giant pulmonary artery aneurysm that caused severe left bronchial obstruction
Authors: Nagato Hisao , Nishiwaki Noboru , Kaneda Kozo , Tamura Nobushige 
Published: April 2020

Abstract
A 63-year-old woman on oral steroids for systemic lupus erythematosus presented with a giant pulmonary artery aneurysm severely compressing the left main bronchus and lung. Her presenting symptom was severe respiratory distress. Surgical graft replacement of the main and branch pulmonary arteries was performed, and her respiratory function improved dramatically. Bronchial obstruction was resolved immediately after the procedure.
How hospitals in mainland China responded to the outbreak of COVID-19 using information technology–enabled services: An analysis of hospital news webpages
Authors: Yan Aihua , Zou Yi , Mirchandani Dinesh A 
Published: April 2020

Abstract

Objective
Many countries have implemented quarantine rules during the global outbreak of coronavirus disease 2019 (COVID-19). Understanding how hospitals can continue providing services in an effective manner under these circumstances is thus important. In this study, we investigate how information technology (IT) helped hospitals in mainland China better respond to the outbreak of the pandemic.

Materials and Methods
We conducted a content analysis of pages published on the websites of the top 50 hospitals in mainland China between January 22 and February 21, 2020. In total, we analyzed 368 pages that the hospitals published during the initial days of the COVID-19 pandemic. The purpose was to identify common themes related to the utilization of IT by these hospitals in response to the pandemic’s outbreak.

Results
We identified 5 focal themes across the webpages published by the hospitals during our study period, including (1) popular medical science education, (2) digitalized hospital processes, (3) knowledge management for medical professionals, (4) telemedicine, and (5) new IT initiatives for healthcare services. Our analysis revealed that Chinese hospitals spent greater effort in promoting popular medical science education in the initial stages of our study period and more on telemedicine in the latter stages.

Discussion
We propose a configurational approach for hospitals to design response strategies to pandemic outbreaks based on their available resources.

Conclusions
Our study provides rich insights for hospitals to better utilize their IT resources and some recommendations for policymaker to better support hospitals in the future.
Biology, Ecology, and Management of Eastern Grape Leafhopper (Hemiptera: Cicadellidae), a Key Pest of Vineyards in North America
Authors: Jarrell Kevin R , Rebek Eric J , Wayadande Astri C , Giles Kristopher L 
Published: April 2020

AbstractEastern grape leafhopper, Erythroneura comes (Say), is a key pest of grapes (Vitales: Vitaceae) in the central and northeastern United States and eastern Canada. This native insect uses its piercing-sucking mouthparts to puncture the mesophyll cells of leaves and suck out the contents, thereby reducing the quality and quantity of fruit. Eastern grape leafhopper has been reported as a vineyard pest for nearly 200 yr and is one of several Erythroneura spp. that feed on grapes in North America. Overwintered adults become active in early spring and may feed on grasses, weeds, and other plants before feeding on grapevines. Although eastern grape leafhopper has an extensive host range, it is only known to oviposit on wild and cultivated grapes. This multivoltine species can outbreak periodically, which has generated much research into improved monitoring and integrated pest management strategies employed throughout its native range. In this review, we describe the biology, life history, population dynamics, and phenology of eastern grape leafhopper and provide a historical account of monitoring and management strategies for this important vineyard pest.
Mon-735: a new low-mass pre-main-sequence eclipsing binary in NGC 2264
Authors: Gillen Edward , Hillenbrand Lynne A , Stauffer John , Aigrain Suzanne , Rebull Luisa , Cody Ann Marie 
Published: April 2020

ABSTRACT
We present Mon-735, a detached double-lined eclipsing binary (EB) member of the ∼3 Myr old NGC 2264 star-forming region, detected by Spitzer. We simultaneously model the Spitzer light curves, follow-up Keck/HIRES radial velocities, and the system’s spectral energy distribution to determine self-consistent masses, radii, and effective temperatures for both stars. We find that Mon-735 comprises two pre-main-sequence M dwarfs with component masses of M = 0.2918 ± 0.0099 and 0.2661 ± 0.0095 M⊙, radii of R = 0.762 ± 0.022 and 0.748 ± 0.023 R⊙, and effective temperatures of Teff = 3260 ± 73 and 3213 ± 73 K. The two stars travel on circular orbits around their common centre of mass in P = 1.9751388 ± 0.0000050 d. We compare our results for Mon-735, along with another EB in NGC 2264 (CoRoT 223992193), to the predictions of five stellar evolution models. These suggest that the lower mass EB system Mon-735 is older than CoRoT 223992193 in the mass–radius diagram (MRD) and, to a lesser extent, in the Hertzsprung–Russell diagram (HRD). The MRD ages of Mon-735 and CoRoT 223992193 are ∼7–9 and 4–6 Myr, respectively, with the two components in each EB system possessing consistent ages.
Stellar coronal mass ejections – II. Constraints from spectroscopic observations
Authors: Odert P , Leitzinger M , Guenther E W , Heinzel P 
Published: April 2020

ABSTRACT
Detections of stellar coronal mass ejections (CMEs) are still rare. Observations of strong Balmer line asymmetries during flare events have been interpreted as being caused by CMEs. Here, we aim to estimate the maximum possible Balmer line fluxes expected from CMEs to infer their detectability in spectroscopic observations. Moreover, we use these results together with a model of intrinsic CME rates to infer the potentially observable CME rates for stars of different spectral types under various observing conditions, as well as the minimum required observing time to detect stellar CMEs in Balmer lines. We find that generally CME detection is favoured for mid- to late-type M dwarfs, as they require the lowest signal-to-noise ratio for CME detection, and the fraction of observable-to-intrinsic CMEs is largest. They may require, however, longer observing times than stars of earlier spectral types at the same activity level, as their predicted intrinsic CME rates are lower. CME detections are generally favoured for stars close to the saturation regime, because they are expected to have the highest intrinsic rates; the predicted minimum observing time to detect CMEs on just moderately active stars is already &gt;100 h. By comparison with spectroscopic data sets including detections as well as non-detections of CMEs, we find that our modelled maximum observable CME rates are generally consistent with these observations on adopting parameters within the ranges determined by observations of solar and stellar prominences.
Chandra observations of the planetary nebula IC 4593
Authors: Toalá J A , Guerrero M A , Bianchi L , Chu Y-H , De Marco O 
Published: April 2020

ABSTRACT
The Advanced CCD Imaging Spectrometer (ACIS-S) camera on board the Chandra X-ray Observatory has been used to discover a hot bubble in the planetary nebula (PN) IC 4593, the most distant PN detected by Chandra so far. The data are used to study the distribution of the X-ray-emitting gas in IC 4593 and to estimate its physical properties. The hot bubble has a radius of ∼2 arcsec and is found to be confined inside the optically bright innermost cavity of IC 4593. The X-ray emission is mostly consistent with that of an optically thin plasma with temperature kT ≈ 0.15 keV (or TX ≈ 1.7 × 106 K), electron density ne ≈ 15 cm−3, and intrinsic X-ray luminosity in the 0.3–1.5 keV energy range LX = 3.4 × 1030 erg s−1. A careful analysis of the distribution of hard (E &gt;0.8 keV) photons in IC 4593 suggests the presence of X-ray emission from a point source likely associated with its central star (CSPN). If this was the case, its estimated X-ray luminosity would be LX, CSPN = 7 × 1029 erg s−1, fulfilling the log(LX, CSPN/Lbol) ≈ −7 relation for self-shocking winds in hot stars. The X-ray detection of the CSPN helps explain the presence of high-ionization species detected in the ultraviolet spectra as predicted by stellar atmosphere models.
The 2019 super-Eddington outburst of RX J0209.6−7427: detection of pulsations and constraints on the magnetic field strength
Authors: Vasilopoulos G , Ray P S , Gendreau K C , Jenke P A , Jaisawal G K , Wilson-Hodge C A , Strohmayer T E , Altamirano D , Iwakiri W B , Wolff M T , Guillot S , Malacaria C , Stevens A L 
Published: April 2020

ABSTRACT
In 2019 November, MAXI detected an X-ray outburst from the known Be X-ray binary system RX J0209.6−7427 located in the outer wing of the Small Magellanic Cloud. We followed the outburst of the system with NICER, which led to the discovery of X-ray pulsations with a period of 9.3 s. We analysed simultaneous X-ray data obtained with NuSTAR and NICER, allowing us to characterize the spectrum and provide an accurate estimate of its bolometric luminosity. During the outburst, the maximum broad-band X-ray luminosity of the system reached (1–2) × 1039 erg s−1, thus exceeding by about one order of magnitude the Eddington limit for a typical 1.4 M⊙ mass neutron star (NS). Monitoring observations with Fermi/GBM and NICER allowed us to study the spin evolution of the NS and compare it with standard accretion torque models. We found that the NS magnetic field should be of the order of 3 × 1012 G. We conclude that RX J0209.6−7427 exhibited one of the brightest outbursts observed from a Be X-ray binary pulsar in the Magellanic Clouds, reaching similar luminosity level to the 2016 outburst of SMC X-3. Despite the super-Eddington luminosity of RX J0209.6−7427, the NS appears to have only a moderate magnetic field strength.
Intra-abdominal hypertension in early post-kidney transplantation period is associated with impaired graft function
Authors: Dupont Vincent , Debrumetz Alexandre , Leguillou Adrien , Morland David , Wynckel Alain , Colosio Charlotte , Mokri Laetitia , Schvartz Betoul , Vuiblet Vincent , Larre Stephane , Barbe Coralie , Rieu Philippe 
Published: April 2020

AbstractBackgroundModerate hyperhydration is often achieved in the early post-kidney transplantation period. Whether this strategy could lead to the development of intra-abdominal hypertension (IAH) has never been assessed so far. We aimed to study the incidence of IAH after kidney transplantation and its association with graft function recovery.MethodsWe conducted a prospective monocentric study among patients undergoing kidney transplantation at the University Hospital of Reims between May 2017 and April 2019. Intravesical pressure (IVP) was monitored every 8 h from Day 0 to 3.ResultsA total of 107 patients were enrolled. Among 55 patients included in the analysis, 74.5% developed IAH. Body mass index &gt;25 kg/m2 was associated with IAH development {odds ratio [OR] 10.4 [95% confidence interval (CI) 2.0–52.9]; P = 0.005}. A previous history of peritoneal dialysis was protective [OR 0.06 (95% CI 0.01–0.3); P = 0.001]. IAH Grades III and IV occurred in 30.9% of patients and correlated with higher Day 3 creatininaemia (419.6 ± 258.5 versus 232.5 ± 189.4 μmol/L; P = 0.02), higher delayed graft function incidence (41.2 versus 7.9%; P = 0.04), lower Kirchner index measured using scintigraphy (0.47 ± 0.09 versus 0.64 ± 0.09; P = 0.0005) and decreased Day 30 estimated glomerular filtration rate (35.8 ± 18.8 versus 52.5 ± 21.3, P = 0.05). IAH patients had higher fluid balance (P = 0.02). Evolution of IVP correlated with weight gain (P &lt; 0.01) and central venous pressure (P &lt; 0.001).ConclusionsIAH is frequent after kidney transplantation and IAH Grades III and IV are independently associated with impaired graft function. These results question current haemodynamic objectives and raise for the first time interest in intra-abdominal pressure monitoring in these patients.Clinical Trial NotationClinicalTrials.gov identifier: NCT03478176
Value of [18F]-FDG positron emission tomography in patients with recurrent glioblastoma receiving bevacizumab
Authors: Graham Maya S , Krebs Simone , Bale Tejus , Domfe Kwaku , Lobaugh Stephanie M , Zhang Zhigang , Dunphy Mark P , Kaley Thomas , Young Robert J 
Published: April 2020

Abstract

Background
Treatment of recurrent glioblastoma (GBM) with bevacizumab can induce MRI changes that confound the determination of progression. We sought to determine the value of [18F]-fluorodeoxyglucose (FDG) positron emission tomography (PET) in GBM patients receiving bevacizumab at the time of suspected progression and, thereby, its utility as a potential prognostic adjunct in progressive disease.

Methods
This retrospective study included patients who underwent brain FDG PET within 4 weeks of receiving bevacizumab for recurrent GBM with suspected progression. Volumes-of-interest were placed over the reference lesion with measurement of maximum standardized uptake value (SUVmax), peak standardized uptake value (SUVpeak), metabolic tumor volume, total lesion glycolysis (TLG), and tumor-to-normal contralateral white matter ratios (TNR-WM). Tumors were additionally categorized as non-avid or avid based on qualitative FDG uptake. Associations between baseline variables and overall survival (OS) were examined using univariable and multivariable Cox proportional hazards regression, with P &lt; .05 considered significant.

Results
Thirty-one patients were analyzed. Qualitative FDG uptake was significantly associated with OS (P = .03), with a median OS of 9.0 months in non-avid patients versus 4.5 months in avid patients. SUVmax, SUVpeak, TNR-WM, and TLG were significantly associated with OS (P &lt; .001, TLG: P = .009). FDG avidity and SUVmax remained significantly associated with OS (P = .046 and .048, respectively) in the multivariable analysis including age, KPS, and MGMT status. Dichotomizing patients using an SUVmax cutoff of 15.3 was associated with OS (adjusted P = .048).

Conclusion
FDG PET is a promising imaging tool to further stratify prognosis in recurrent GBM patients on antiangiogenic therapy.