bluuebunny/crossref_metadata_2025_split huggingface dataset:
Implementasi Text Mining Untuk Analisis Opini Publik Terhadap Calon Presiden
Authors: Kartikasari Trya Sovi , Setiawan Hendry , Lucky Tirma Irawan Paulus 
Published: November 2020

Sistem presidensial merupakan salah satu bentuk demokrasi di Indonesia. Sistem tersebut menitikberatkan pada penyelenggaraan pemilihan umum presiden dan wakilnya yang dilakukan secara langsung oleh rakyat. Tingkat terpilihnya seorang presiden dapat dilihat dari opini publik yang beredar, salah satunya pada media sosial yang juga merupakan bagian dari kampanye. Dalam penelitian ini akan dianalisa opini yang berkaitan dengan elektabilitas calon presiden dari media sosial Twitter dari media sosial Twitter menggunakan metode Naïve Bayes Classifier (NBC) dan menentukan faktor-faktor yang terbentuk dari opini menggunakan Principal Component Analysis (PCA). Data opini dari media sosial Twitter didapatkan menggunakan kata kunci “Jokowi” dan “Prabowo”. Sebagian opini tersebut dipilih sebagai data latih untuk didapatkan kelas bersentimen negatif dan positif. Setelah proses pelatihan, dilakukan proses terhadap data uji dan data validasi. Hasil akurasi untuk data uji topik Jokowi pada tweet bersentimen positif mendapatkan akurasi sebesar 88.63% dan negatif sebesar 91.06%. Sementara untuk Prabowo bersentimen positif mendapatkan akurasi sebesar 88.58% dan negatif sebesar 80.37%. Rerata akurasi untuk keseluruhan topik adalah adalah 86.89%. Untuk mendapatkan faktor pada setiap sentimen, dilakukan proses perhitungan nilai PCA. Setiap sentimen tersebut kemudian dilakukan analisis faktor oleh pakar, yakni didapatkan 20 faktor yang sudah berhasil diinterpretasikan oleh pakar.
Exosome-mediated miR-1290 regulation of SLU7 affects hepatocellular carcinoma process
Authors: Zeng Yong , Tan Xingqin , Gong Jianpin , Liu Zhichuan 
Published: November 2020

Abstract
Objective: Liver cancer, as a very common malignant tumor in clinic, has a high incidence rate and a poor prognosis. Understanding the relationship of exosomes miR-1290 and SLU7 with liver cancer may be a breakthrough in the diagnosis and treatment of liver cancer in the future.Methods: Altogether 64 patients with liver cancer and 58 healthy people in our hospital were collected, and liver cancer cells were purchased. qPCR was used to detect the mRNA levels of miR-1290 and SLU7. Western blot was used to detect the relative expression of SLU7 protein, and the effect of exosomes miR-1290 and SLU7 on the biological behavior of liver cancer cells was analyzed. Double fluorescein reporter was used to verify the targeted relationship between miR-1290 and SLU7.Results: miR-1290 was highly expressed in liver cancer (P< 0.050) and SLU7 was poorly expressed in liver cancer (P< 0.050). Exosomes miR-1290 could inhibit the relative expression of SLU7 protein (p < 0.050), promote the proliferation, invasion, migration and epithelial-mesenchymal transformation of liver cancer cells, and reduce the ability of apoptosis (P< 0.050). Double luciferase report and rescue experiment confirmed that miR-1290 and SLU7 had targeted relationship (P< 0.050).Conclusion: Exosomes miR-1290 may be a potential target for the diagnosis and treatment of liver cancer in the future through targeting inhibition of SLU7 in the occurrence and development of liver cancer.
The Effect of Dual Strain Probiotic on the Growth and Feeding Intolerance in Premature Neonates of Kabul city: A Randomized Clinical Trial
Authors: Aslamzai Mansoor , Raheen Abdul Rahim , Hamidi Mohammad Farouq , Hassin Zemary 
Published: November 2020

Abstract
Background: Prematurity is prevalent worldwide and accounts as the leading cause of neonatal death. Slow weight gain, feeding intolerance, prolong hospital stay, recurrent infection and high mortality are significant issues for preterm neonates. Dual strain probiotic can manage such complication of prematurity. Objective: To evaluate the effectiveness of dual strain probiotic on weight gaining and feeding intolerance of preterm neonates in Kabul city.Method: This randomized controlled trial conducted at Neonatal units of Malalai and Maiwand Hospitals in Kabul city. Finally, 84 preterm neonates assessed for the effect of dual strain probiotic. The clinical parameters were daily weight gain and the risk of feeding intolerance and neonatal mortality. Statically analysis was performed by SPSS 20. Results: Forty-two premature neonates in dual strain probiotic group and another forty-two preterm babies in control group were investigated. The preterm neonates in probiotic versus control group had mean age (7.2±6.3 vs 6.8±3.3 day; P=0.63), weight (1495.5±273 vs 1476.2±273 g; P=0.75) and gestational age (33.6±2.1 vs 32.9±2.4 week; p=0.14). Sex distribution in probiotic group was (boys 54.4%, girl 45.2%) and in control group was (boys 50%, girl 50%). Both groups were observed for mean days of (17.6±6.4 vs 18.8±6.3; P=0.45). The premature newborns in probiotic versus control group had mean daily weight gain (15.4±9.4 vs 10±7.9g; CI=1.75-9.31, P=0.005), risk of feeding intolerance (RR=0.65, CI=0.1-4.1, P=0.6) and risk of mortality during hospital stay (RR=0.65, CI=0.1-0.41, P=0.8).Conclusion: Preterm neonates in our country had slow growth than high-income countries. Dual strain probiotic significantly improved the daily weight gain of premature neonates. However, probiotic also decreased the risk of feeding intolerance and death during hospital stay but these findings were not statistically significant.Clinical trial registration number: This RCT was registered in the Australian New Zealand Clinical Trials Registry with the number of ACTRN12620000538943. Date of Registration is first May 2020. https://www.anzctr.org.au/ACTRN12620000538943.aspx
A Retrospective Analysis of the Effects of Different Analgesics on the Pain and Functional Recovery of Patients with Traumatic Thoracolumbar Fractures in the Peri-Treatment Period
Authors: Yuan Hao , Chang Quan-Yuan , Chen Jie , Wang Ya-Ting , Gan Zong-Jin , Wen Song , Li Ting-Ting , Xiong Liu-Lin 
Published: November 2020

Abstract
Background: There are few literatures analyze the effects of traumatic thoracolumbar vertebral fractures on prognosis pain and functional recovery from the perspective of analgesics used in the perioperative period, and lack of clinical guidance on the application of analgesics for its surgery.Objective: To analyze the effects of peri-treatment analgesics on pain and recovery status of patients with thoracolumbar fractures combined with network pharmacology.Methods: 719 patients with thoracolumar fractures were collected and divided into acetaminophen dihydrocodeine group (n=476), celecoxib group (n=130) and etoricoxib group (n=113) according to the use of analgesics. The visual analogue scale (VAS) was used to measure the degree of pain the number of days of the use of three analgesics, the postoperative Oswestry dysfunction index (ODI) scores and some Japanese orthopedics Association score (JOA) were recorded. The targets of three analgesics were obtained by Drugbank, and the target of acute pain caused by fracture is obtained by Genecards, then Venn diagram of the intersection genes was drawn through Venn. Protein interaction mapping (PPI) was obtained by using STRING database, the functional enrichment analysis of Gene Ontology (GO) and the enrichment analysis of Kyoto Encyclopedia of Genes and Genomics (KEGG) pathway were carried out based on Metascape database.Results: The codeine group was higher than celecoxib and etoricoxib groups in the number of days of preoperative and postoperative analgesic use, VAS score difference on the preoperative and postoperative, and postoperative ODI index (all P<0.01). There exist no statistically conspicuous differences among the three groups in basic characteristics, injury and intraoperative conditions, ODI score except ODI index, three JOA marks, and postoperative walking ability (P>0.05). Identify 3332 disease targets. PPI network analysis identified OPRM1, OPRK1, TRPV1, PTGS1 and PTGS2 as the core targets for acetaminophen dihydrocodeine in the treatment. Conclusion: If multiple analgesic modes are adopted in the perioperative analgesic use, and the use of acetaminophen dihydrocodeine in combination with anti-inflammatory analgesics, the duration of analgesic use is expected to be shortened as far as possible to reduce the pain degree in postoperative recovery, providing a reference for the optimal use of anesthetics for clinical traumatic thoracolumbar fractures.
Health Insurance Ownership among Mollucans in Indonesia
Authors: Laksono Agung Dwi , Sillehu Sahrir , Khoiri Abu , Wulandari Ratna Dwi 
Published: November 2020

Abstract
Health insurance is an effort to transfer risks to increase public access to the use of health services. The study was aimed at analyzing factors related to health insurance ownership among Mollucans in Indonesia. The sample size was 788 respondents. The variables analyzed included health insurance ownership, age group, gender, education level, employment status. The final stage was employed multinomial logistic regression. The results show Mollucans in the ≤19 age group were 0.182 times more likely than Mollucans in the ≥ 50 age group to have government-run type health insurance. Mollucans in the 20-29 age group were 0.219 times more likely than Mollucans in the ≥ 50 age group to have government-run health insurance. On the other hand, Mollucans with primary education was 0.196 times more likely than Mollucans with higher education to have a government-run type of health insurance. Mollucans with secondary education was 0.415 times more likely than Mollucans with higher education to have government-run health insurance. Meanwhile, unemployed Mollucans have a probability of 0.358 times compared to employed Mollucans to have the government-run type of health insurance. Finally, unemployed Mollucans are 0.056 times more likely than employed Mollucans to have private-run health insurance. It was concluded that 3 proven variables associated with health insurance ownership among Moluccans in Indonesia, namely age group, education level, and employment status.
Does Low-Temperature Sterilization Affect the Geometrical Properties of 3D Printed Models and Surgical Guides from a Reconstruction of CT Images using the Mimics Innovation Suite?
Authors: Toro Mauricio , Cardona Aura , Restrepo Daniel , Buitrago Laura 
Published: November 2020

Abstract
Background: Fused deposition anatomical models and surgical guides are the embodiment of digital surgical planning. Although the models are primarily produced for training or simulation, they often touch the patient tissues and fluids while the guides are always in close contact with the patient. Therefore, sterility is a requirement but not at the expense of losing dimensions and shape. In the present study, we assessed the effects of sterilization with vaporized hydrogen peroxide on the geometrical properties and accuracy of 3D-printed pieces reconstructed and processed using the Mimics Innovation Suite.Methods: computer tomography (CT) scan images from 16 patients were selected, eight from patients with maxillary and mandibular defects for the anatomical models, and eight from patients with mandibular defects for the cutting guides. The devices were manufactured in medical-grade acrylonitrile butadiene styrene from the primary files of the CT scans reconstructions. Two scanning processes were repeated after the production, one before and the other after sterilization. The dimensional error was estimated in each step of the process by comparing the scanned images of the printed pieces between before and after the sterilization process with the original design.Results: The average of the estimated mean differences between the printed pieces before and after sterilization were -0,011 ± 0,252 mm (95%CI -0,011; -0,010) for the models and 0,003 ± 0,057 mm (95%CI 0,002; 0,003) for the guides. Regarding the dimensional error of the sterilized parts compared to the original design, the estimated mean differences were -0,082 ± 0,626 mm (95%CI -0,083; -0,081) for the models and 0,126 ± 0,205 mm (95%CI 0,126; 0,127) for the cutting guides.Conclusion: The geometrical properties of the 3D-printed anatomical models and cutting guides designed with the Mimics Innovation Suite and manufactured in ABS was not affected after the process of low-temperature sterilization. The devices maintained dimensional stability after the sterilization but also showed high reliability relating to the original design.
Characterisation of a Highly Potent and Near Pan-Neutralising Anti-HIV Monoclonal Antibody Expressed in Tobacco Plants
Authors: Moore Catherine Margaret , Grandits Melanie , Grünwald-Gruber Clemens , Altmann Friedrich , Kotouckova Maria , Teh Audrey Y.H. , Ma Julian K.C. 
Published: November 2020

Abstract
Background HIV remains one of the most important health issues worldwide, with almost 40 million people living with HIV. Although patients develop antibodies against the virus, its high mutation rate allows evasion of immune responses. Some patients, however, produce antibodies that are able to bind to, and neutralise different strains of HIV. One such ‘broadly neutralising’ antibody is ‘N6’. Identified in 2016, N6 can neutralise 98% of HIV-1 isolates with a median IC50 of 0.066 µg/mL. This neutralisation breadth makes N6 a very promising therapeutic candidate. Results N6 was expressed in a glycoengineered line of N. benthamiana plants (pN6) and compared to the mammalian cell-expressed equivalent (mN6). Expression at 49mg/kg (fresh leaf tissue) was achieved in plants, although extraction and purification are more challenging than for most plant-expressed antibodies. N-glycoanalysis demonstrated the absence of xylosylation and a reduction in α(1,3)-fucosylation that are typically found in plant glycoproteins. The N6 light chain contains a potential N-glycosylation site, which was modified and displayed more α(1,3)-fucose than the heavy chain. The binding kinetics of pN6 and mN6, measured by surface plasmon resonance, were similar for HIV gp120. pN6 had a 10-fold higher affinity for FcγRIIIa, which was reflected in an antibody-dependent cellular cytotoxicity assay, where pN6 induced a more potent response from effector cells than that of mN6. pN6 demonstrated the same potency and breadth of neutralisation as mN6, against a panel of HIV strains. Conclusions The successful expression of N6 in tobacco supports the prospect of developing a low-cost, low-tech production platform for a monoclonal antibody cocktail to control HIV in low-to middle income countries.
Glutamic Acid Reshapes The Phytobiome To Protect Plants Against Pathogens
Authors: Kim Da-Ran , Jeon Chang-Wook , Cho Gyeongjun , Thomashow Linda , Weller David , Paik Man-Jeong , Lee Yong Bok , Kwak Youn-Sig 
Published: November 2020

Abstract
Background: The physiology and growth of plants are strongly influenced by their associated microbiomes. Conversely, the composition of the phytobiome is flexible, responding to the state of the host and raising the possibility that it can be engineered to benefit the plant. However, technology for engineering the structure of the microbiome is not yet available.Results: Here we show that glutamic acid reshapes the plant microbial community and enriches populations of Streptomyces, a functional core microbe, both above and below ground, in strawberry and tomato. Upon application of glutamic acid, the population size of Streptomyces increased dramatically in the anthosphere and the rhizosphere. At the same time, diseases caused by species of Fusarium were significantly reduced in both habitats. Plant resistance-related genes were not activated, suggesting that glutamic acid modulates the microbiome community directly, rather than activating the host’s own protective mechanisms.Conclusions: Much is known about the structure of plant-associated microbial communities, but little has been learned about how the community composition and complexity are controlled. Our results demonstrate that the microbiome community can be engineered and unlock the mode of action of glutamic acid.
WITHDRAWN: Genotype-Dependent Recruitment of the Strawberry Holobiome
Authors: Cellini Antonio , Sangiorgio Daniela , Donati Irene , Ferrari Erika , Tanunchai Benjawan , Wahdan Sara Fareed Mohamed , Sadubsarn Dolaya , Farneti Brian , Buscot Francois , Spinelli Francesco , Purahong Witoon 
Published: November 2020

Abstract
BackgroundCultivated strawberry (Fragaria × ananassa Duch., fam. Rosaceae) is an important fruit crop, greatly appreciated for its aroma and nutraceutical properties. Niche-specific characterisation of plant microbiome, from rhizosphere to aboveground plant organs, is crucial to understand the influence of structure and function of the microbial communities on plant phenotype, performances and disease resistance. Strawberry cultivation is challenged by a large variety of pathogens, which cause substantial economic losses and require the frequent application of pesticides. Biological control is a promising and safer alternative to the use of xenobiotic pesticides. Biological control agents isolated from the microbiome of the host plant may have a superior efficacy in comparison to non-indigenous microbial inoculants. Therefore, the characterization of the native microbiome along different plant compartments is a key step for the successful microbial manipulation in farmlands. Results Here, we provide the first comprehensive description of the soil, rhizosphere, root and aerial parts microbiome of three commercially important strawberry cultivars (‘Darselect’, ‘Elsanta’ and ‘Monterey’) under cultural conditions. The fungal and bacterial microbiomes were functionally characterised to investigate their influence on plant disease tolerance, plant mineral nutrient content and fruit quality. The core microbiome included 24 bacteria and 15 fungal operative taxon units which were present in all compartments and plant genotypes. However, both plant organ and genotype had a significant role in assembling the microbial communities. The microbial community assemblage across different soil and plant compartments significantly correlated with disease resistance, mineral nutrient content in the plant and with fruit quality parameters. Interestingly, only the disease tolerant genotype ‘Monterey’ was able to recruit Pseudomonas fluorescens in all plant organs and to establish symbiosis with the arbuscular mycorrhiza Rhizophagus irregularis. These two species include several strains acting as pathogen biocontrol agents, plant growth promoters and plant defence inducers. Conclusions Altogether, our study provides the first comprehensive view of strawberry microbiome in relation to plant genotype, health and nutritional status and fruit quality parameters, shedding light on potential practical applications to increase the sustainability of crop production.
Determination of the most important characteristics in bread wheat grain yields under salinity stress
Authors: Ragab Khaled , Kheir Ahmed 
Published: November 2020

Abstract
Background

Salinity has adverse effects on crop production in arid and semi-arid regions but so far, less attention has been paid to this impact on wheat characteristics. A pot experiment was carried out in cage-house to determine the most important characteristics in wheat under wide range of salinity using the stepwise regression analysis. The data collected on five groups of characteristics (i.e. seedling, phonology, spike, physiology, yield and its contributions).
Results

The findings showed that the salinity levels below 3.5 dSm− 1 triggered stimulation in the growth of wheat seedling, while the salinity rates increased by more than 3.5 dSm− 1 significantly reduced all the seedling characteristics studied. The salinity level of 3.5 dS m− 1 resulted in an earliness percentage of 4.2, 4.9, 3.8 and 2.2% respectively in the developmental stages of booting, heading, anthesis and maturity. Meanwhile, rising salinity levels up to 10.5 dSm− 1 resulted in a 21.7% decrease in emergence speed and a delay of 3.9, 10.8 and 8.5% in booting, heading and anthesis developmental stages respectively. Salinity stress decreased the flag leaf area and increased concentration of chlorophyll pigments, where the percentage increase for chlorophyll a was higher compared with chlorophyll b and total chlorophyll. The stress of salinity decreased all the studied spike characteristics with significant effect on the number of spike− 1 and spike kernel weights.
Conclusions

Salinity stress decreased yield and other contributes where its effect was strong on plant height, root dry weight, biological and grain yield. The stepwise regression showed that biological yield, spike kernels weight and number of spikes pot− 1 characteristics under both normal and salinity stress are the important selection criteria of high grain yield.
The Inhibition of EV71 Virus-Induced Apoptosis by ZVAD Through ROS and mTOR Pathway
Authors: Xu Tiantian , Li Yinghua , Guo Min , Zhao Mingqi , Chen Haiyang , Wang Changbing , Kuang Lu , Xiao Misi , Lin Zhengfang , Zhu Bing 
Published: November 2020

Abstract
Background: Emerging evidence that Enterovirus 71 (EV71) infection closely related to infection with the generation of apoptosis. Excess apoptosis exacerbates inflammatory reactions and can even result in the death of the cell. The ZVAD is a broad caspase inhibitor, can prevent apoptosis and regulate the survival in many different cell types. Methods: The aims of this project were to elucidate the mechanism of the ZVAD in anti-EV71 virus.Results: Our data showed that reduced viral replication was observed after treated with ZVAD in EV71 infected Vero and SK-N-SH cells. Meanwhile, Vero and SK-N-SH cells infected with EV71virus were both exhibit higher cells viability in the presence of ZVAD. Additionally, ZVAD can decrease the apoptosis, ROS and inflammation induced by EV71 in the infected Vero cells. However, ZVAD may play an antiviral role in SK-N-SH cells through the mTOR signaling pathwayConclusions: In addition to inhibiting caspase, ZVAD also inhibits apoptosis by regulating ROS and inflammation cytokines to achieve antiviral effect.