bluuebunny/crossref_metadata_2025_split huggingface dataset:
Third‐Generation Anti–Cyclic Citrullinated Peptide Antibodies Improve Prediction of Clinical Arthritis in Individuals at Risk of Rheumatoid Arthritis
Authors: Di Matteo Andrea , Mankia Kulveer , Duquenne Laurence , Mahler Michael , Corscadden Diane , Mbara Katie , Garcia‐Montoya Leticia , Nam Jacqueline L. , Emery Paul 
Published: June 2020

ObjectiveTo 1) determine the prevalence of anti–cyclic citrullinated peptide 3 (anti‐CCP3) antibodies in anti‐CCP2 antibody–positive (anti‐CCP2+) at‐risk individuals, and 2) explore the additional value of anti‐CCP3 antibodies in anti‐CCP2+ at‐risk individuals for predicting progression to inflammatory arthritis.MethodsStored serum samples obtained from 347 anti‐CCP2+ (BioPlex 2200; Bio‐Rad) at‐risk individuals without clinical synovitis were tested for anti‐CCP3 antibodies. Anti‐CCP2 titers were categorized as low or high, and anti‐CCP3 titers were categorized as negative, low, or strong. Progression to inflammatory arthritis was defined as the development of clinical synovitis in ≥1 joint. Only subjects with ≥1 follow‐up visit were included in the progression analysis (n = 291).ResultsIn the 347 samples included, anti‐CCP3 antibody titers tended to be either negative (n = 138 [39.7%]) or strongly positive (n =189 [54.4%]), with very few subjects showing a low titer (n = 20 [5.7%]). In contrast, for anti‐CCP2 antibodies, more low titers were observed (n = 103 [29.7%]). Eighty‐eight of 291 subjects (30.2%) developed inflammatory arthritis. The rate of progression to inflammatory arthritis in the low‐titer anti‐CCP2 group and the high‐titer anti‐CCP2 group fell from 7.5% to 3.3% and from 38.9% to 9.8%, respectively, when anti‐CCP3 was negative. Progression in the high‐titer anti‐CCP2 group increased from 38.9% to 48.4% when anti‐CCP3 was strongly positive. The area under the curve was 0.72 for anti‐CCP2 (95% confidence interval [95% CI] 0.66, 0.78) and 0.76 for anti‐CCP3 (95% CI 0.70, 0.81) for assessment of progression. In the multivariable analysis, the odds ratio for the development of inflammatory arthritis in anti‐CCP3+ subjects was 1.73 (95% CI 1.20, 2.51) (P < 0.01).ConclusionAnti‐CCP3 antibodies improve the prediction of inflammatory arthritis in anti‐CCP2+ at‐risk individuals. The impact of anti‐CCP3 antibody status for the risk stratification of individuals with high‐titer anti‐CCP2 is particularly notable.
A new distribution‐free adaptive sample size control chart for a finite production horizon and its application in monitoring fill volume of soft drink beverage bottles
Authors: Khatun Mahfuza , Khoo Michael B.C. , Saha Sajal , Castagliola Philippe 
Published: June 2020

AbstractNonparametric control charts have received increasing attention in process monitoring. In this article, a new nonparametric sign (SN) control chart with variable sample size (VSS) for a finite horizon process is developed. The novelty of this research lies in the incorporation of the VSS technique into the nonparametric SN chart for a finite horizon process, hence, resulting in the development of a more sensitive nonparametric short run chart. The statistical performance of the new nonparametric VSS SN control chart is evaluated and compared with the existing fixed sample size (FSS) SN chart for a finite horizon process. The charts' performances are compared using the truncated average run length (TARL) and truncated standard deviation of the run length (TSDRL) criteria. The results obtained show that the nonparametric VSS SN short run chart is always quicker than the FSS SN short run chart in detecting process shifts for various underlying process distributions, hence, reducing scrap and rework cost. Finally, an application of the proposed control charting scheme is shown through a real‐life example on the fill volume of soft drink beverage bottles.
Networks of planar symmetric periodic orbits in a barred galaxy model
Authors: Zotos Euaggelos E. , Jung Christof , Papadakis Konstandinos E. , Saeed Tareq 
Published: June 2020

AbstractWe present the networks of planar symmetric periodic orbits in a single barred galaxy model. More specifically, we determine how the numerical value of the bar's semimajor axis affects not only the positions but also the linear stability of the periodic solutions. The atlas of the periodic orbits, with multiplicity up to 10, is presented on the (x, E) plane so as to be able to understand the importance of the total orbital energy on the periodic networks. For every orbital family, we also compute the horizontal and vertical critical solutions of the system, from which new periodic families bifurcate. Our analysis indicates that several periodic families contain orbits, which qualify as x1 type orbits and stabilize the barred structure of the galaxy. Furthermore, the numerical results suggest that the semimajor axis of the bar highly influences the overall orbital properties of the dynamical system.
Graphitic‐Based Solid‐State Supercapacitors: Enabling Redox Reaction by In Situ Electrochemical Treatment
Authors: Amjadipour Mojtaba , Su Dawei , Iacopi Francesca 
Published: June 2020

AbstractInvited for this month's cover picture is the group of Integrated Nano Systems Lab (INSys Lab), part of the Centre for Clean Energy Technology, University of Technology Sydney. The cover picture illustrates an efficient in situ pathway to generate and attach oxygen functional groups to graphitic electrodes for supercapacitors by inducing hydrolysis of water molecules within the gel electrolyte. Read the full text of the Article at 10.1002/batt.201900204.
Adapting with Microbial Help: Microbiome Flexibility Facilitates Rapid Responses to Environmental Change
Authors: Voolstra Christian R. , Ziegler Maren 
Published: June 2020

AbstractAnimals and plants are metaorganisms and associate with microbes that affect their physiology, stress tolerance, and fitness. Here the hypothesis that alteration of the microbiome may constitute a fast‐response mechanism to environmental change is examined. This is supported by recent reciprocal transplant experiments with reef corals, which have shown that their microbiome adapts to thermally variable habitats and changes over time when transplanted into different environments. Further, inoculation of corals with beneficial bacteria increases their stress tolerance. But corals differ in their ability to flexibly associate with different bacteria. How scales of microbiome flexibility may reflect different metaorganism adaptation mechanisms is discussed and future directions for research are pinpointed. It is posited that microbiome flexibility is a broad phenomenon that contributes to the ability of organisms to respond to environmental change. Importantly, adapting with microbial help may provide an alternate route to organismal adaptation that facilitates rapid responses.
A comprehensive model for the diffusion and hybridization processes of nucleic acid probes in fluorescence in situ hybridization
Authors: Lima Joana F. , Maia Paulo , T. Magalhães Beatriz , Cerqueira Laura , Azevedo Nuno F. 
Published: June 2020

AbstractFluorescence in situ hybridization (FISH) has been extensively used in the past decades for the detection and localization of microorganisms. However, a mechanistic approach of the whole FISH process is still missing, and the main limiting steps for the hybridization to occur remain unclear. In here, FISH is approached as a particular case of a diffusion‐reaction kinetics, where molecular probes (MPs) move from the hybridization solution to the target RNA site within the cells. Based on literature models, the characteristic times taken by different MPs to diffuse across multiple cellular barriers, as well as the reaction time associated with the formation of the duplex molecular probe‐RNA, were estimated. Structural and size differences at the membrane level of bacterial and animal cells were considered. For bacterial cells, the limiting step for diffusion is likely to be the peptidoglycan layer (characteristic time of 7.94 × 102 – 4.39 × 103 s), whereas for animal cells, the limiting step should be the diffusion of the probe through the bulk (1.8–5.0 s) followed by the diffusion through the lipid membrane (1 s). The information provided here may serve as a basis for a more rational development of FISH protocols in the future.
iFR uncovers profound but mostly reversible ischemia in CTOs and helps to optimize PCI results
Authors: Kayaert Peter , Coeman Mathieu , Drieghe Benny , Bennett Johan , McCutcheon Keir , Dens Jo , Ungureanu Claudiu , Zivelonghi Carlo , Agostoni Pierfrancesco , Bataille Yoann , de Hemptinne Quentin , Gevaert Sofie , De Pauw Michel , Haine Steven 
Published: June 2020

AbstractObjectivesThe study aimed to demonstrate through instant wave‐free ratio (iFR) measurements that myocardium distal to a chronic total occlusion (CTO) is ischemic, that ischemia is reversible by PCI, and that iFR assessment after PCI can be used to optimize PCI results.BackgroundThe greatest benefit of revascularization is found in patients with low fractional flow reserve. In patients with CTOs, iFR measurement may be more appropriate to evaluate ischemia as it does not require maximal microvascular vasodilation, which may be hampered by microvascular dysfunction.MethodsThe iFR was measured in 81 CTO patients, both pre‐ and post‐PCI in 63 patients, and only post‐PCI in the following 18 patients. A pressure wire pullback was performed post‐PCI if iFR ≤0.89.ResultsThe first 63 patients all had significant ischemia distal to the CTO with a median iFR of 0.33 [0.22; 0.44], improving significantly post‐PCI to a median iFR of 0.93 [0.89;0.96] (p < .001). In the complete cohort, the median iFR post‐PCI was 0.93 [0.86;0.96] but still ≤0.89 in 23 patients (30%). 12 of these patients had further PCI optimization because of a residual focal pressure gradient on pullback, after which only two had a final iFR ≤0.89.ConclusionsIn CTO patients with an indication for PCI, iFR consistently demonstrated profound myocardial ischemia. Successful PCI immediately relieved ischemia in 70% of patients. In the remaining 30% of cases, a manual iFR pullback proved helpful in guiding further optimization of the PCI result.
Coordination Chemistry and Methylation of Mixed‐Substituted Tetraphosphetanes (RP−PtBu)2 (R=Ph, Py)
Authors: Schoemaker Robin , Kossatz Philipp , Schwedtmann Kai , Hennersdorf Felix , Weigand Jan J. 
Published: June 2020

AbstractSynthesis of mixed‐substituted tetraphosphetanes (RP−PtBu)2 (R=Ph (4), Py (5); Py=2‐pyridyl) is achieved from the condensation of dipyrazolylphosphanes RPpyr2 (R=Py (1), Ph (3); pyr=3,5‐dimethylpyrazolyl) as P1‐building block (R−P) and tBuPH2 in an equimolar ratio. Compound 5 is of special interest since the presence of two pyridyl‐substituents as well as the P4‐core allows for a rich coordination chemistry with coinage metal salts [Cu(MeCN)4][OTf], Ag[OTf] and in situ formed [Au(tht)][OTf] (tht=tetrahydrothiophene). Both tetraphosphetanes undergo alkylation reaction with MeOTf to give a series of tetraphosphetanium and tetraphosphetanediium triflate salts with additional methylation of the pyridyl‐moiety in case of 5 resulting in interesting novel cyclic trications. Harsh reaction condition and an excess of MeOTf converts 5 into the cyclic trication [‐P(MePy)PMe2P(MePy)PtBu‐]3+ (133+; MePy=1‐methylpyridiniumyl) through the elimination of isobutene. This salt undergoes a complicated rearrangement reaction involving a P−P/P−P bond metathesis to form trication [‐P(MePy)3PtBu‐]3+ (173+) when reacted with Me2PPMe2.
Controlling PROTACs with Light
Authors: Verma Shreya , Manna Debasish 
Published: June 2020

AbstractProteolysis targeting chimeras, PROTACs, are emerging as a powerful strategy for exerting exogenous control over protein levels, allowing small molecules to exploit the ubiquitin–proteasome pathway for targeted protein degradation. This highlight focuses on the fusion of photochemistry with these bifunctional compounds, which has provided a novel pathway for spatiotemporally tuning the activation of PROTACs in the form of their photocaged and photoswitchable versions. Photocaged PROTACs consist of a hindered optolabile group that detaches only upon irradiation at a specific wavelength, releasing the active PROTAC. These modified PROTACs are inactive in the dark. Photoswitchable PROTACs are photoisomerizable molecules with azobenzene linkages that are active in either the cis or trans form and inactive in the other. The isomers interconvert upon irradiation with an appropriate wavelength of light and relax to the thermodynamically stable isomer in the dark or with another wavelength of light. Although photocaged PROTACs only permit activation control for protein degradation, photoswitching PROTACs offer reversible activation and deactivation by using suitable wavelengths of light.
Reprogramming of Human Hepatic Non‐Parenchymal Cells: Step‐by‐Step Protocol
Authors: Kirchner Varvara A. , Twaroski Kirk , Wysoglad Kelli , Algoo Jenna , LeCluyse Edward L. , Song Gi‐Won , Tak Eunyoung , Chen Weili , Lee Sung‐Gyu , Pruett Timothy L. , Tolar Jakub 
Published: June 2020

AbstractHuman induced pluripotent stem cells (h‐iPSCs) represent a potentially unlimited source for the generation of human hepatocyte‐like cells (h‐iHLCs) for the establishment of platforms to study drug‐induced hepatotoxicity, liver disease modeling, and ultimately the application of h‐iHLCs in treatment of patients with end‐stage liver disease. To understand the impact of donor‐specific factors on the generation of h‐iHLCs, the model for the direct comparison of h‐iHLCs and primary human hepatocytes (PHHs) from the same human donor is needed. This study proposes a step‐by‐step protocol for plating, expansion, and characterization of primary human hepatic non‐parenchymal cells (h‐NPCs) isolated from the human liver, the reprogramming of generated h‐NPCs into h‐iPSCs and subsequent differentiation of reprogrammed h‐iPSCs into h‐iHLCs. The ultimate goal is to compare the gene expression involved in hepatocyte metabolism between h‐iHLCs and PHHs from the same human donor thus eliminating interdonor variability. This newly developed protocol of h‐NPC culture, expansion, and reprogramming into h‐iPSCs allows: (1) utilization of a single organ source (i.e., liver) for isolation of PHHs and h‐NPCs and (2) the in‐depth study of donor factors involved in the generation of h‐iHLCs with direct comparison to PHHs from the same donor. © 2020 Wiley Periodicals LLC.Basic Protocol 1: Plating and expansion of human hepatic NPCs in cultureBasic Protocol 2: Reprogramming of h‐NPCs to h‐NPC‐derived induced pluripotent stem cells (h‐iPSCs)Basic Protocol 3: Culture, passaging, and freezing of h‐iPSCsSupport Protocol 1: Confirmation of h‐NPC ability to uptake Sendai virus: GFP‐Sendai infectionSupport Protocol 2: Characterization of h‐NPCs amenable to transduction with Sendai particlesSupport Protocol 3: Characterization of h‐iPSCs: Clearance of viral reprogramming vectorsSupport Protocol 4: Preparation of Matrigel‐coated plates
Application of essential oil of Pistacia atlantica Gum, polypropylene and silica nanoparticles as a new milk packaging
Authors: Ellahi Hasan , Khalili Sadrabad Elham , Hekmatimoghaddam Seyedhossein , Jebali Ali , Sarmast Elham , Akrami Mohajeri Fateme 
Published: June 2020

AbstractThe aim of current study was to investigate the antimicrobial effect of gum essential oil of Pistacia atlantica (wild pistachio) tree (GEO) and design a new film based on polypropylene polymer coated with silica nanoparticles and GEO. The antimicrobial activity of the packaging film was evaluated with or without milk on Staphylococcus aureus, Salmonella enterica, Escherichia coli, and Listeria monocytogenes during 35 days. The results showed that GEO has significant antibacterial properties. It was most effective on Salmonella enterica, while its effect on Listeria monocytogenes was the weakest. Antimicrobial activity of the film without milk showed no significant differences among the different sizes of nanoparticles used (0.05, 0.025, and 0.051 g) (p ≥ .05). It can be concluded that polypropylene incorporated with GEO and silica nanoparticles active film had antimicrobial properties up to 35 days, while using with milk or without milk. Therefore, this type of packaging is effective to enhance the shelf life of milk.
Risk stratification in oral squamous cell carcinoma using staging of the eighth American Joint Committee on Cancer: Systematic review and meta‐analysis
Authors: Almangush Alhadi , Pirinen Matti , Youssef Omar , Mäkitie Antti A. , Leivo Ilmo 
Published: June 2020

AbstractThe eighth edition of the American Joint Committee on Cancer (AJCC8) staging manual has major changes in oral squamous cell carcinoma (OSCC). We searched PubMed, OvidMedline, Scopus, and Web of Science for studies that examined the performance of AJCC8 in OSCC. A total of 40 808 patients were included in the studies of our meta‐analysis. A hazard ratio (HR) of 1.87 (95%CI 1.78‐1.96) was seen for stage II, 2.65 (95%CI 2.51‐2.80) for stage III, 3.46 (95%CI 3.31‐3.61) for stage IVa, and 7.09 (95%CI 4.85‐10.36) for stage IVb. A similar gradual increase in risk was noted for the N classification. For the T classification, however, there was a less clear variation in risk between T3 and T4. AJCC8 provides a good risk stratification for OSCC. Future research should examine the proposals introduced in the published studies to further improve the performance of AJCC8.
Modeling the Hepatology Workforce in the United States: A Predicted Critical Shortage
Authors: Russo Mark W. , Fix Oren K. , Koteish Ayman A. , Duggan Katie , Ditmyer Marcia , Fuchs Michael , Chung Raymond T. , Reddy Gautham 
Published: June 2020

Background and Aims
Liver disease is prevalent in the United States, and as the population ages, an increasing number of patients are anticipated to present for care. The state of the current hepatology workforce and future demand for hepatology providers is not known. The aim of this study was to model future projections for hepatology workforce demand.

Approach and Results
A workforce study of hepatology providers in the United States was completed using primary and secondary data sources. An integrated workforce framework model was used that combined socioeconomic factors that drive economic demand, epidemiological factors that drive need, and utilization rates of health care services. Supply and demand projections were calculated for adult and pediatric hepatology professionals. Sensitivity analyses were conducted to cover the feasible range of these assumptions. An electronic survey of American Association for the Study of Liver Diseases (AASLD) members whose practice included 50% or more hepatology was conducted. In 2018, the adult and pediatric workforce included 7,296 and 824 hepatology providers, respectively, composed of hepatologists, gastroenterologists, and advanced practice providers whose practice was ≥50% hepatology. The modeling analysis projects that in 2023, 2028, and 2033, there will be shortages of 10%, 23%, and 35% adult hepatology providers, respectively, and 19%, 20%, and 16% pediatric hepatology providers, respectively. In sensitivity analyses, a shortage of hepatology providers is predicted even under optimistic assumptions. Among the respondents to the survey, the median age was higher among gastroenterologists and general hepatologists compared with transplant hepatologists. The most common category treated by transplant hepatologists was general hepatology.

Conclusions
There is an impending critical shortage of adult and pediatric hepatology providers. Strategies are needed to encourage clinicians to pursue hepatology, especially in areas outside of transplant centers.
Priority Pharmaceutical Micropollutants and Feasible Management Initiatives to Control Water Pollution from the Perspective of Stakeholders in Metropolis of Southern Brazil
Authors: Barcellos Demian da Silveira , Helwig Karin , Gervasoni Ronald , Teedon Paul , Possetti Gustavo Rafael Collere , Bollmann Harry Alberto 
Published: June 2020

Abstract
The search for common agreement between stakeholders is one of the biggest challenges when solving environmental problems. There are different views, perceptions, knowledge, and sectoral goals for these stakeholders. In complex environmental problems, such as the management of pharmaceutical micropollutants, it is essential to establish the intersectoral and individual sector priorities for a collective approach to the problem. This research aimed to identify the priority micropollutants for intervention initiatives and the management goals of the stakeholders involved in the “product chain” of pharmaceuticals in the region of Curitiba, Brazil. First, the most consumed pharmaceutical micropollutants in the region and those previously detected in water were identified, and a “long list” of 40 pharmaceuticals was drawn up for stakeholder prioritization. Stakeholders of the region were selected by intentional sampling and were invited to a workshop that was designed to list, by consensus, the priority micropollutants and objectives for their management. The event was attended by 37 stakeholders from different sectors. It was divided into 2 stages: the 1st stage was a multisectoral discussion to select the priority pharmaceutical micropollutants; the 2nd stage was a sectoral discussion to establish management objectives to control and reduce the presence of these pollutants in waters. The meeting generated a coherent prioritization of pharmaceuticals where ethinyl estradiol, ciprofloxacin, ibuprofen, diclofenac, estradiol, caffeine, and fluoxetine were prioritized and their importance was justified. The main sectoral goals prioritized were related to the drug take-back program, the characterization of the presence of pharmaceuticals in the sanitation cycle, and the creation of a permanent multisector discussion environment for the theme. The multisectoral definition, established by consensus, of management priorities is promising and this strategic approach can be replicated in other developing countries. Integr Environ Assess Manag 2020;16:955–967. © 2020 SETAC