bluuebunny/crossref_metadata_2025_split huggingface dataset:
Parents Need More Support: A Qualitative Study of the Experiences of Australian Parents Who Are Waiting for Surgical Intervention for Their Children With Otitis Media
Authors: Stephens Jacqueline H , O’Keefe Maree , Schembri Mark , Baghurst Peter A 
Published: October 2019

Objective: To explore the experiences, expectations, and motivations of parents/caregivers of children with otitis media who were booked to undergo tympanostomy tube insertion. Method: A cross-sectional cohort study was conducted using semistructured interviews with 39 parents. Interviews were conducted via telephone and analyzed for key themes. Results: Three themes emerged that incorporated a range of subthemes: (1) the impact of the child’s underlying condition on the family, (2) the cues and prompts that influenced parents to seek intervention, and (3) the parents’ expectations of the health-care system. The child’s otitis media disrupted the day-to-day functioning of the family and the child’s well-being, but despite this, the families found ways to adapt and cope. Parents were influenced by their friends, family, and medical practitioners when making treatment decisions and had differing expectations of the health-care system. Conclusion: Parents need support during their child’s illness to help with pressures placed on the family and also in making health-care decisions for their child. Clinicians should consider these issues when discussing treatment options with parents.
Identification of amino acid residues that are crucial for FXIII-A intersubunit interactions and stability
Authors: Li Bojun , Kohler Hans P. , Schroeder Verena 
Published: October 2019

Abstract
Coagulation factor XIII (FXIII) is the main stabilizer of the fibrin clot. It circulates in plasma as a tetramer of two A-subunits and two B-subunits. Under physiological conditions, FXIII-A exists as a dimer (FXIII-A2). The interactions between the FXIII-A-subunits that stabilize the FXIII-A2 dimer are not fully understood. We therefore designed a systematic approach to identify amino acid residues crucial for the expression and stability of FXIII-A2. Based on the available FXIII-A2 crystal structure, we identified 12 amino acid residues forming intersubunit salt bridges and 21 amino acid residues forming hydrogen bonds between the two A-subunits. We chose 10 amino acid residues that form 5 particularly strong interactions, performed site-directed mutagenesis, and expressed the mutants in CHO cells. Disruption of these interactions by single mutation of Lys257, Lys113, Asp343, Glu401, or Asp404 abolished the expression of properly folded, soluble, and functional FXIII-A in CHO cells. On the contrary, mutation of Glu111, Arg100, or Asn112 had no significant effect on FXIII-A expression. Our results suggest that 4 intersubunit interactions (Arg11-Asp343, Lys113-Asp367, Lys257-Glu401, and Arg260-Asp404) are essential for the stability of FXIII-A2. Our findings are supported by reported mutations at Lys257, Arg260, and Asp404 found in patients with congenital FXIII-A deficiency.
A Nuclear Factor Y (NFY) Site Positively Regulates the Human CD34 Stem Cell Gene
Authors: Radomska Hanna S. , Satterthwaite Anne B. , Taranenko Natalie , Narravula Sailaja , Krause Diane S. , Tenen Daniel G. 
Published: October 2019

AbstractProper regulation of the human CD34 gene requires a combinatorial action of multiple proximal and long-range, ciselements. This report shows that, like the murine CD34 5′ untranslated region (UTR), the corresponding region of the human CD34 gene is necessary for optimal promoter activity. We localized the most critical element of this region to base pairs +48/+75. Through oligonucleotide competition and antibody supershift experiments in electrophoretic mobility shift assays, we found that this sequence contains a binding site (CCAAT box) for the transcription factor NFY (nuclear factor Y), a factor mediating cell type-specific and cell-cycle regulated expression of genes. Mutating this site led to a 5-fold decrease in CD34 promoter activity in transient transfection experiments. Interestingly, NFY binds adjacently to the earlier identified c-myb binding site. Here we show that both binding sites are important for CD34 promoter function: mutating either site alone decreased CD34 promoter-driven reporter gene activity 4-fold. We also show that the integrity of the c-myb binding site is necessary for stabilization of NFY binding to its site. Such cooperation between c-myb, which is expressed in early hematopoietic cells, and NFY, which is expressed in many cell types, might contribute to specific activation of CD34 in stem cells. The CCAAT box motif was also noted in the 5′ UTR of the murine CD34 gene, however, NFY did not bind to this region. Thus, our results indicate that the functional similarities between the human and murine CD34 5′ UTRs are achieved through different molecular mechanism(s).
Megakaryocytes package contents into separate α-granules that are differentially distributed in platelets
Authors: Battinelli Elisabeth M. , Thon Jonathan N. , Okazaki Ross , Peters Christian G. , Vijey Prakrith , Wilkie Adrian R. , Noetzli Leila J. , Flaumenhaft Robert , Italiano Joseph E. 
Published: October 2019

Key Points
Mouse megakaryocytes can differentially sort and package endocytosed fibrinogen and endostatin into distinct α-granules. Platelet progenitors contain subpopulations of α-granules.
Investigating differences in trunk muscle activity in non-specific chronic low back pain subgroups and no-low back pain controls during functional tasks: a case-control study
Authors: Hemming Rebecca , Sheeran Liba , van Deursen Robert , Sparkes Valerie 
Published: October 2019

Abstract

Background
Trunk muscle dysfunction is often regarded as a key feature of non-specific chronic low back pain (NSCLBP) despite being poorly understood and variable with increases, decreases and no change in muscle activity reported. Differences in thoraco-lumbar kinematics have been observed in motor control impairment NSCLBP subgroups (Flexion Pattern, Active Extension Pattern) during static postures and dynamic activities. However, potential differences in muscle activity during functional tasks has not been established in these subgroups to date.

Methods
A case-control study design recruited 50 NSCLBP subjects (27 Flexion Pattern, 23 Active Extension Pattern) and 28 healthy individuals. Surface electromyography determined muscle activity during functional tasks: reaching upwards, step-down, step-up, lifting and replacing a box, stand-to-sit, sit-to-stand, bending to retrieve (and returning from retrieving) a pen from the floor. Normalised (% sub-maximal voluntary contraction) mean amplitude electromyography of bilateral musculature (transversus abdominis/internal oblique, external oblique, superficial lumbar multifidus and longissimus thoracis) was analysed using Kruskal-Wallis and post-hoc Mann-Whitney U tests.

Results
Transversus abdominis/internal oblique activity was significantly increased in the Flexion Pattern group compared to controls during stand-to-sit (p = 0.009) on the left side only. External oblique activity was significantly greater in the Active Extension Pattern group compared to controls during box lift (p = 0.016) on the right side only. Significantly greater activity was identified in the right Superficial lumbar multifidus during step up (p = 0.029), reach up (p = 0.013) and box replace (p = 0.007) in the Active Extension Pattern group compared to controls. However left-sided superficial lumbar multifidus activity was significantly greater in the Flexion Pattern group (compared to controls) only during stand-to-sit (p = 0.009). No significant differences were observed in longissimus thoracis activity bilaterally during any task. No significant differences between NSCLBP subgroups were observed.

Conclusions
Muscle activity in these NSCLBP subgroups appears to be highly variable during functional tasks with no clear pattern of activity identified. The findings reflect inconsistencies and variability in trunk muscle activity previously observed in these NSCLBP subgroups. Further work evaluating ratios of muscle activity and changes in muscle activity throughout task duration is warranted.
Cervical epidural hematoma after spinal manipulation therapy: a case report
Authors: Chen Qian , Feng Jun-fei , Tang Xin , Li Yu-ling , Chen Lu , Chen Guo 
Published: October 2019

Abstract

Background
Cervical spinal manipulation therapy is a common non-invasive treatment for neck pain and stiffness, and has been widely used in the population. However, most people do not pay attention to the potential risks of neck manipulation, such as ligament damage, fractures, and spinal cord injuries. Epidural hematoma is a disease in which blood accumulates in the epidural space of the vertebral body. This disease is usually caused by trauma or iatrogenic surgery, and may be associated with blood coagulopathies, neoplasms, or degenerative spinal disease. Reports of epidural hematoma caused by cervical spinal manipulation are rare.

Case presentation
We present the case of a patient with tetraplegia and spinal shock after neck manipulation. A physical examination of the patient on admission found tenderness in the neck and increased muscle tension in both upper limbs. The superficial sensation of the upper limb disappeared, but the deep sensation still remained. The lower extremity had 0/5 power on both sides. The sensation below the T2 level completely disappeared. A cervical magnetic resonance imaging scan showed an acute posterior epidural hematoma from the C3–T3 vertebrae. Ultimately, the patient underwent emergency hematoma removal and showed partial improvement in symptoms of paralysis during follow-up.

Conclusions
Although spinal manipulation is simple and neck pain is common and recurrent in the general population, the basic condition and disease history of patients should be determined before manipulation. For high-risk patients, caution should be applied for cervical spinal manipulation or it should be prohibited. For a suspected hematoma, MRI should be used at an early stage to diagnose and locate the hematoma.
Robust hypergraph regularized non-negative matrix factorization for sample clustering and feature selection in multi-view gene expression data
Authors: Yu Na , Gao Ying-Lian , Liu Jin-Xing , Wang Juan , Shang Junliang 
Published: October 2019

AbstractBackgroundAs one of the most popular data representation methods, non-negative matrix decomposition (NMF) has been widely concerned in the tasks of clustering and feature selection. However, most of the previously proposed NMF-based methods do not adequately explore the hidden geometrical structure in the data. At the same time, noise and outliers are inevitably present in the data.ResultsTo alleviate these problems, we present a novel NMF framework named robust hypergraph regularized non-negative matrix factorization (RHNMF). In particular, the hypergraph Laplacian regularization is imposed to capture the geometric information of original data. Unlike graph Laplacian regularization which captures the relationship between pairwise sample points, it captures the high-order relationship among more sample points. Moreover, the robustness of the RHNMF is enhanced by using the L2,1-norm constraint when estimating the residual. This is because the L2,1-norm is insensitive to noise and outliers.ConclusionsClustering and common abnormal expression gene (com-abnormal expression gene) selection are conducted to test the validity of the RHNMF model. Extensive experimental results on multi-view datasets reveal that our proposed model outperforms other state-of-the-art methods.
Human mitochondrial genome compression using machine learning techniques
Authors: Wang Rongjie , Zang Tianyi , Wang Yadong 
Published: October 2019

Abstract

Background
In recent years, with the development of high-throughput genome sequencing technologies, a large amount of genome data has been generated, which has caused widespread concern about data storage and transmission costs. However, how to effectively compression genome sequences data remains an unsolved problem.

Results
In this paper, we propose a compression method using machine learning techniques (DeepDNA), for compressing human mitochondrial genome data. The experimental results show the effectiveness of our proposed method compared with other on the human mitochondrial genome data.

Conclusions
The compression method we proposed can be classified as non-reference based method, but the compression effect is comparable to that of reference based methods. Moreover, our method not only have a well compression results in the population genome with large redundancy, but also in the single genome with small redundancy. The codes of DeepDNA are available at https://github.com/rongjiewang/DeepDNA.
An ARFID case report combining family-based treatment with the unified protocol for Transdiagnostic treatment of emotional disorders in children
Authors: Eckhardt Sarah , Martell Carolyn , Duncombe Lowe Kristina , Le Grange Daniel , Ehrenreich-May Jill 
Published: October 2019

Abstract

Background
This case report discusses the presentation and treatment of a nine-year-old female with a history of significant weight loss and food refusal using a combined approach of Family-Based Treatment (FBT) and the Unified Protocol for Transdiagnostic Treatment of Emotional Disorders in Children (UP-C).

Case presentation
The patient was diagnosed with avoidant/restrictive food intake disorder (ARFID), separation anxiety disorder, and a specific phobia of choking, and subsequently treated with a modified version of FBT, in conjunction with the UP-C. At the end of treatment, improvements were seen in the patient’s weight and willingness to eat a full range of foods. Decreases in anxiety regarding eating/choking, fears of food being contaminated with gluten, and fears of eating while being away from parents were also observed.

Conclusions
These findings highlight promising results from this combined treatment approach, referred to as FBT + UP for ARFID. Further research is needed to evaluate the use of this treatment in patients presenting with a variety of ARFID symptoms.
Vitiligo susceptibility at workplace and in daily life: the contribution of oxidative stress gene polymorphisms
Authors: Chiarella Pieranna 
Published: October 2019

AbstractObjectiveVitiligo is a frequently underestimated and little known dermal disease whose symptoms appear as white patches on several skin areas of the body. In this review, the impact of idiopathic and chemical-induced vitiligo at workplace and in daily life is discussed. Also, the influence of selected oxidative stress gene polymorphisms on melanocyte damage is described to understand their involvement in the disease.MethodsA PubMed search was carried out to select the journal articles reporting an association between specific oxidative stress polymorphic genes and vitiligo.ResultsThe double-null glutathione S-transferase T1 and M1 genotypes are associated with vitiligo while the relationship between nuclear factor erythroid 2-related factor 2, heme oxygenase, catalase and superoxide dismutase gene polymorphisms and the disease should be confirmed by further studies.ConclusionsThe polymorphic genes analysed here may have a role in the susceptibility of patients affected by vitiligo, while little is known about the affected workers, due to the lack of epidemiologic data on these subjects. However, the similarity of the skin lesions observed in both groups might have in common some genetic factors making all these individuals susceptible to the development of vitiligo, regardless of the disease-triggering factor.
Omega-3 and polyunsaturated fat for prevention of depression and anxiety symptoms: systematic review and meta-analysis of randomised trials
Authors: Deane Katherine H. O. , Jimoh Oluseyi F. , Biswas Priti , O'Brien Alex , Hanson Sarah , Abdelhamid Asmaa S. , Fox Chris , Hooper Lee 
Published: October 2019

BackgroundThere is strong public belief that polyunsaturated fats protect against and ameliorate depression and anxiety.AimsTo assess effects of increasing omega-3, omega-6 or total polyunsaturated fat on prevention and treatment of depression and anxiety symptoms.MethodWe searched widely (Central, Medline and EMBASE to April 2017, trial registers to September 2016, ongoing trials updated to August 2019), including trials of adults with or without depression or anxiety, randomised to increased omega-3, omega-6 or total polyunsaturated fat for ≥24 weeks, excluding multifactorial interventions. Inclusion, data extraction and risk of bias were assessed independently in duplicate, and authors contacted for further data. We used random-effects meta-analysis, sensitivity analyses, subgrouping and Grading of Recommendations, Assessment, Development and Evaluations (GRADE) assessment.ResultsWe included 31 trials assessing effects of long-chain omega-3 (n= 41 470), one of alpha-linolenic acid (n= 4837), one of total polyunsaturated fat (n= 4997) and none of omega-6. Meta-analysis suggested that increasing long-chain omega-3 probably has little or no effect on risk of depression symptoms (risk ratio 1.01, 95% CI 0.92–1.10,I2= 0%, median dose 0.95 g/d, duration 12 months) or anxiety symptoms (standardised mean difference 0.15, 95% CI 0.05–0.26,I2= 0%, median dose 1.1 g/d, duration 6 months; both moderate-quality evidence). Evidence of effects on depression severity and remission in existing depression were unclear (very-low-quality evidence). Results did not differ by risk of bias, omega-3 dose, duration or nutrients replaced. Increasing alpha-linolenic acid by 2 g/d may increase risk of depression symptoms very slightly over 40 months (number needed to harm, 1000).ConclusionsLong-chain omega-3 supplementation probably has little or no effect in preventing depression or anxiety symptoms.Declaration of interestL.H. and A.A. were funded to attend the World Health Organization Nutrition Guidance Expert Advisory Group (NUGAG) Subgroup on Diet and Health meetings and present review results. The authors report no other conflicts of interest.
Hippocampal Neurochemical Profile and Glucose Transport Kinetics in Patients With Type 1 Diabetes
Authors: Bednařík Petr , Henry Pierre-Gilles , Khowaja Amir , Rubin Nathan , Kumar Anjali , Deelchand Dinesh , Eberly Lynn E , Seaquist Elizabeth , Öz Gülin , Moheet Amir 
Published: October 2019

Abstract

Context
Longstanding type 1 diabetes (T1D) may lead to alterations in hippocampal neurochemical profile. Upregulation of hippocampal glucose transport as a result of recurrent exposure to hypoglycemia may preserve cognitive function during future hypoglycemia in subjects with T1D and impaired awareness of hypoglycemia (IAH). The effect of T1D on hippocampal neurochemical profile and glucose transport is unknown.

Objective
To test the hypothesis that hippocampal neurochemical composition is altered in T1D and glucose transport is upregulated in T1D with IAH.

Design and participants
Hippocampal neurochemical profile was measured with single-voxel magnetic resonance spectroscopy at 3T during euglycemia in 18 healthy controls (HC), 10 T1D with IAH, and 12 T1D with normal awareness to hypoglycemia (NAH). Additionally, 12 HC, 8 T1D-IAH, and 6 T1D-NAH were scanned during hyperglycemia to assess hippocampal glucose transport with metabolic modeling.

Setting
University medical center.

Main Outcome Measures
Concentrations of hippocampal neurochemicals measured during euglycemia and ratios of maximal transport rate to cerebral metabolic rate of glucose (Tmax/CMRGlc), derived from magnetic resonance spectroscopy–measured hippocampal glucose as a function of plasma glucose.

Results
Comparison of hippocampal neurochemical profile revealed no group differences (HC, T1D, T1D-IAH, and T1D-NAH). The ratio Tmax/CMRGlc was not significantly different between the groups, T1D-IAH (1.58 ± 0.09) and HC (1.65 ± 0.07, P = 0.54), between T1D-NAH (1.50 ± 0.09) and HC (P = 0.19), and between T1D-IAH and T1D-NAH (P = 0.53).

Conclusions
Subjects with T1D with sufficient exposure to recurrent hypoglycemia to create IAH did not have alteration of Tmax/CMRglc or neurochemical profile compared with participants with T1D-NAH or HC.
Advanced Glycation End Products: Building on the Concept of the “Common Soil” in Metabolic Disease
Authors: Ruiz Henry H , Ramasamy Ravichandran , Schmidt Ann Marie 
Published: October 2019

AbstractThe role of advanced glycation end products (AGEs) in promoting and/or exacerbating metabolic dysregulation is being increasingly recognized. AGEs are formed when reducing sugars nonenzymatically bind to proteins or lipids, a process that is enhanced by hyperglycemic and hyperlipidemic environments characteristic of numerous metabolic disorders including obesity, diabetes, and its complications. In this mini-review, we put forth the notion that AGEs span the spectrum from cause to consequence of insulin resistance and diabetes, and represent a “common soil” underlying the pathophysiology of these metabolic disorders. Collectively, the surveyed literature suggests that AGEs, both those that form endogenously as well as exogenous AGEs derived from environmental factors such as pollution, smoking, and “Western”-style diets, contribute to the pathogenesis of obesity and diabetes. Specifically, AGE accumulation in key metabolically relevant organs induces insulin resistance, inflammation, and oxidative stress, which in turn provide substrates for excess AGE formation, thus creating a feed-forward–fueled pathological loop mediating metabolic dysfunction.
On rough convergence of ρ-Cauchy sequence of triple sequences
Authors: Esi Ayhan , Aiyub M. , Subramanian N. , Esi Ayten 
Published: October 2019

AbstractIn this paper we define and study rough convergence of triple sequences and the set of rough limit points of a triple sequence. We also investigate the relations between the set of cluster points and the set of rough limit points of Cauchy sequences of triple sequence spaces.
Effect of Temperature on Effluent Quality in a Biological Wastewater Treatment Process
Authors: Tejaswini E. , Uday Bhaskar Babu G. , Seshagiri Rao A. 
Published: October 2019

Abstract
In the present study, the temperature effects on the organic carbon and nitrogen removal in an activated sludge process are evaluated. Benchmark Simulation Model No.1 (BSM1) based on activated sludge process is used for all the simulation purposes. A steady state simulation is performed to analyze the effluent concentrations with varying kinetic parameters obtained from different temperature coefficients over a wide range of temperatures from 15 °C to 35 °C. The temperature coefficient ‘a’ is assumed to have different set of values specific to the kinetic parameters, namely, Maximum heterotrophic growth rate

μ

m
H

\left( {{\mu _{mH}}} \right)

, Maximum autotrophic growth rate

μ

m
A

\left( {{\mu _{mA}}} \right)

, Heterotrophic decay rate

b
H

\left( {{b_H}} \right)

, Autotrophic decay rate

b
A

\left( {{b_A}} \right)

. The effluent concentration defined in terms of Chemical Oxygen Demand (COD), Biochemical Oxygen Demand (BOD), Total Suspended Solids (TSS), Total Nitrogen (TN) and Ammonia are observed to be significantly changing with a change in the kinetic parameters which are in turn a strong function of temperature coefficient. Emphasis is laid on the temperature range of 25–30 °C as it is commonly the most operated temperature range in a WWTP in India. It is also noticed that at temperatures <20 °C and >30 °C, the effluent limitations are violated from the standard values.