bluuebunny/crossref_metadata_2025_split huggingface dataset:
HRTEM study of interfacial structure in SiCp/A390 composites
Authors: Wang Zhen , Wang Aiqin , Han Huihui , Xie Jingpei 
Published: April 2020

Abstract
SiCp/A390 composite was fabricated by powder metallurgy method and hot extrusion process. The interfacial microstructure of SiCp/A390 composites after heat treatment was characterized by HRTEM. The results show that the interface between SiC and Al matrix has an orientation relationship of [4

5
¯

10]SiC//[0

1
¯

1]Al, (10

1
¯

0) SiC//(

1
¯

11) Al and forming a semi-coherent interface. The crystallographic orientation relationship between SiC and Si in the SiCp/A390 composites is [010]SiC//[001]Si, (004)SiC//(220)Si. The interplanar spacing of Al matrix on lattice plane (111) is close to the twice interplanar spacing of the Si phase on lattice plane (400). They keep a semi-coherent crystal plane, with an orientation relationship of [01

1
¯

]Al//[001]Si, (111)Al//(400)Si.
Neuroprotective Effects of Grape Seed Procyanidins on Ethanol-Induced Injury and Oxidative Stress in Rat Hippocampal Neurons
Authors: Jin Wenyang , Sun Mizhu , Yuan Bingbing , Wang Runzhi , Yan Hongtao , Qiao Xiaomeng 
Published: April 2020

Abstract

Aims
Ethanol is a small molecule capable of interacting with numerous targets in the brain, the mechanisms of which are complex and still poorly understood. Studies have revealed that ethanol-induced hippocampal neuronal injury is associated with oxidative stress. Grape seed procyanidin (GSP) is a new type of antioxidant that is believed to scavenge free radicals and be anti-inflammatory. This study evaluated the ability and mechanism by which the GSP improves ethanol-induced hippocampal neuronal injury.

Methods
Primary cultures of hippocampal neurons were exposed to ethanol (11, 33 and 66 mM, 1, 4, 8, 12 and 24 h) and the neuroprotective effects of GSP were assessed by evaluating the activity of superoxide dismutase (SOD), the levels of malondialdehyde (MDA) and lactate dehydrogenase (LDH) and cell morphology.

Results
Our results indicated that GSP prevented ethanol-induced neuronal injury by reducing the levels of MDA and LDH, while increasing the activity of SOD. In addition, GSP increased the number of primary dendrites and total dendritic length per cell.

Conclusion
Together with previous findings, these results lend further support to the significance of developing GSP as a therapeutic tool for use in the treatment of alcohol use disorders.
Platelet-Rich Fibrin Improves Fat Graft Survival Possibly by Promoting Angiogenesis and Adipogenesis, Inhibiting Apoptosis, and Regulating Collagen Production
Authors: Yu Panxi , Zhai Zhen , Lu Haibin , Jin Xiaolei , Yang Xiaonan , Qi Zuoliang 
Published: April 2020

Abstract

Background
Platelet-rich fibrin (PRF) can promote fat graft survival, but limited data are currently available, and the underlying mechanism of this effect has not yet been explained.

Objectives
The aim of this study was to explore the mechanism by which PRF promotes fat graft survival, from the aspects of angiogenesis, adipogenesis, cellular apoptosis, and collagen production.

Methods
Nude mice were randomly assigned to a PRF group (subcutaneously injected with PRF and fat in the ratio of 1:5 by volume) and a control group (subcutaneously injected with normal saline and fat in the ratio of 1:5 by volume). On days 0, 3, 7, 14, 21, and 28 after transplantation, graft samples (n = 12) were obtained for quantification of target growth factors. In weeks 1, 2, 3, and 4 after transplantation, graft samples (n = 12) were obtained for the following evaluations. The volume and weight retention rates were calculated; gene and protein expression of vascular endothelial growth factor A (VEGF-A), peroxisome proliferator–activated receptor γ (PPAR-γ), COL1-A1, and BAX were evaluated; hematoxylin & eosin staining, Masson’s trichrome staining, α smooth muscle actin staining, and perilipin-1 staining were performed to evaluate graft survival.

Results
After transplantation, the concentrations of growth factors produced by the fat increased to varying degrees, and the addition of PRF made these concentration changes ever greater. Compared with the control group, the PRF group had a higher volume and weight retention rate, a higher expression level of VEGF-A and PPAR-γ, a lower expression level of COL1-A1 and BAX, a higher vessel density, less fibrosis, and more viable adipocytes.

Conclusions
PRF can promote autocrine function of the grafted fat to produce more growth factors. It greatly increased fat retention rate, possibly by promoting vascularization and adipogenic differentiation, inhibiting cellular apoptosis, and regulating collagen production.
Portable nanopore analytics: are we there yet?
Authors: Oliva Marco , Milicchio Franco , King Kaden , Benson Grace , Boucher Christina , Prosperi Mattia 
Published: April 2020

Abstract

Motivation
Oxford Nanopore technologies (ONT) add miniaturization and real time to high-throughput sequencing. All available software for ONT data analytics run on cloud/clusters or personal computers. Instead, a linchpin to true portability is software that works on mobile devices of internet connections. Smartphones’ and tablets’ chipset/memory/operating systems differ from desktop computers, but software can be recompiled. We sought to understand how portable current ONT analysis methods are.

Results
Several tools, from base-calling to genome assembly, were ported and benchmarked on an Android smartphone. Out of 23 programs, 11 succeeded. Recompilation failures included lack of standard headers and unsupported instruction sets. Only DSK, BCALM2 and Kraken were able to process files up to 16 GB, with linearly scaling CPU-times. However, peak CPU temperatures were high. In conclusion, the portability scenario is not favorable. Given the fast market growth, attention of developers to ARM chipsets and Android/iOS is warranted, as well as initiatives to implement mobile-specific libraries.

Availability and implementation
The source code is freely available at: https://github.com/marco-oliva/portable-nanopore-analytics.
Brain responsivity provides an individual readout for motor recovery after stroke
Authors: Tscherpel Caroline , Dern Sebastian , Hensel Lukas , Ziemann Ulf , Fink Gereon R , Grefkes Christian 
Published: April 2020

AbstractPromoting the recovery of motor function and optimizing rehabilitation strategies for stroke patients is closely associated with the challenge of individual prediction. To date, stroke research has identified critical pathophysiological neural underpinnings at the cellular level as well as with regard to network reorganization. However, in order to generate reliable readouts at the level of individual patients and thereby realize translation from bench to bedside, we are still in a need for innovative methods. The combined use of transcranial magnetic stimulation (TMS) and EEG has proven powerful to record both local and network responses at an individual’s level. To elucidate the potential of TMS-EEG to assess motor recovery after stroke, we used neuronavigated TMS-EEG over ipsilesional primary motor cortex (M1) in 28 stroke patients in the first days after stroke. Twenty-five of these patients were reassessed after &gt;3 months post-stroke. In the early post-stroke phase (6.7 ± 2.5 days), the TMS-evoked EEG responses featured two markedly different response morphologies upon TMS to ipsilesional M1. In the first group of patients, TMS elicited a differentiated and sustained EEG response with a series of deflections sequentially involving both hemispheres. This response type resembled the patterns of bilateral activation as observed in the healthy comparison group. By contrast, in a subgroup of severely affected patients, TMS evoked a slow and simplified local response. Quantifying the TMS-EEG responses in the time and time-frequency domain revealed that stroke patients exhibited slower and simple responses with higher amplitudes compared to healthy controls. Importantly, these patterns of activity changes after stroke were not only linked to the initial motor deficit, but also to motor recovery after &gt;3 months post-stroke. Thus, the data revealed a substantial impairment of local effects as well as causal interactions within the motor network early after stroke. Additionally, for severely affected patients with absent motor evoked potentials and identical clinical phenotype, TMS-EEG provided differential response patterns indicative of the individual potential for recovery of function. Thereby, TMS-EEG extends the methodological repertoire in stroke research by allowing the assessment of individual response profiles.
Passive immunotherapy for N-truncated tau ameliorates the cognitive deficits in two mouse Alzheimer’s disease models
Authors: Corsetti Veronica , Borreca Antonella , Latina Valentina , Giacovazzo Giacomo , Pignataro Annabella , Krashia Paraskevi , Natale Francesca , Cocco Sara , Rinaudo Marco , Malerba Francesca , Florio Rita , Ciarapica Roberta , Coccurello Roberto , D’Amelio Marcello , Ammassari-Teule Martine , Grassi Claudio , Calissano Pietro , Amadoro Giuseppina 
Published: April 2020

AbstractClinical and neuropathological studies have shown that tau pathology better correlates with the severity of dementia than amyloid plaque burden, making tau an attractive target for the cure of Alzheimer’s disease. We have explored whether passive immunization with the 12A12 monoclonal antibody (26–36aa of tau protein) could improve the Alzheimer’s disease phenotype of two well-established mouse models, Tg2576 and 3xTg mice. 12A12 is a cleavage-specific monoclonal antibody which selectively binds the pathologically relevant neurotoxic NH226-230 fragment (i.e. NH2htau) of tau protein without cross-reacting with its full-length physiological form(s). We found out that intravenous administration of 12A12 monoclonal antibody into symptomatic (6 months old) animals: (i) reaches the hippocampus in its biologically active (antigen-binding competent) form and successfully neutralizes its target; (ii) reduces both pathological tau and amyloid precursor protein/amyloidβ metabolisms involved in early disease-associated synaptic deterioration; (iii) improves episodic-like type of learning/memory skills in hippocampal-based novel object recognition and object place recognition behavioural tasks; (iv) restores the specific up-regulation of the activity-regulated cytoskeleton-associated protein involved in consolidation of experience-dependent synaptic plasticity; (v) relieves the loss of dendritic spine connectivity in pyramidal hippocampal CA1 neurons; (vi) rescues the Alzheimer’s disease-related electrophysiological deficits in hippocampal long-term potentiation at the CA3-CA1 synapses; and (vii) mitigates the neuroinflammatory response (reactive gliosis). These findings indicate that the 20–22 kDa NH2-terminal tau fragment is crucial target for Alzheimer’s disease therapy and prospect immunotherapy with 12A12 monoclonal antibody as safe (normal tau-preserving), beneficial approach in contrasting the early Amyloidβ-dependent and independent neuropathological and cognitive alterations in affected subjects.
Economic Exchange or Gender Identities? Housework Division and Wives’ Economic Dependency in Different Contexts
Authors: Mandel Hadas , Lazarus Amit , Shaby Maayan 
Published: April 2020

Abstract
This paper explores cross-country variation in the relationship between division of housework and wives’ relative economic contribution. Using ISSP 2012 data from 19 countries, we examined the effect of two contextual factors: women’s employment rates, which we link to economic exchange theories; and gender ideology context, which we link to cultural theories. In line with economic-based theories, economic exchange between housework and paid work occurs in all countries—but only in households which follow normative gender roles. However, and consistent with the cultural-based theory of ‘doing gender’, wives undertake more housework than their spouses in all countries—even if they are the main or sole breadwinners. This universal gendered division of housework is significantly more salient in more conservative countries; as the context turns more conservative, the gender gap becomes more pronounced, and the relationship between paid and unpaid work further removed from the economic logic. In gender egalitarian societies, in contrast, women have more power in negotiating housework responsibilities in non-normative gender role households. In contrast to gender ideology, the cross-country variations in women’s employment did not follow the expectations that derive from the economic exchange theory.
First Genome Sequence of the Gunnison’s Prairie Dog (Cynomys gunnisoni), a Keystone Species and Player in the Transmission of Sylvatic Plague
Authors: Tsuchiya Mirian T N , Dikow Rebecca B , Cassin-Sackett Loren 
Published: April 2020

Abstract
Prairie dogs (genus Cynomys) are a charismatic symbol of the American West. Their large social aggregations and complex vocalizations have been the subject of scientific and popular interest for decades. A large body of literature has documented their role as keystone species of western North America’s grasslands: They generate habitat for other vertebrates, increase nutrient availability for plants, and act as a food source for mammalian, squamate, and avian predators. An additional keystone role lies in their extreme susceptibility to sylvatic plague (caused by Yersinia pestis), which results in periodic population extinctions, thereby generating spatiotemporal heterogeneity in both biotic communities and ecological processes. Here, we report the first Cynomys genome for a Gunnison’s prairie dog (C. gunnisoni gunnisoni) from Telluride, Colorado (USA). The genome was constructed using a hybrid assembly of PacBio and Illumina reads and assembled with MaSuRCA and PBJelly, which resulted in a scaffold N50 of 824 kb. Total genome size was 2.67 Gb, with 32.46% of the bases occurring in repeat regions. We recovered 94.9% (91% complete) of the single copy orthologs using the mammalian Benchmarking Universal Single-Copy Orthologs database and detected 49,377 gene models (332,141 coding regions). Pairwise Sequentially Markovian Coalescent showed support for long-term stable population size followed by a steady decline beginning near the end of the Pleistocene, as well as a recent population reduction. The genome will aid in studies of mammalian evolution, disease resistance, and the genomic basis of life history traits in ground squirrels.
High incidence of sea turtle stranding in the southwestern Atlantic Ocean
Authors: Cantor Mauricio , Barreto André Silva , Taufer Renata M , Giffoni Bruno , Castilho Pedro V , Maranho Andrea , Beatriz Carla , Kolesnikovas Christiane , Godoy Daniela , Rogério Daniel W , Dick Jeferson L , Groch Karina R , Rosa Liana , Cremer Marta J , Cattani Pâmela E , Valle Rodrigo R , Domit Camila 
Published: April 2020

AbstractSea turtles are globally threatened due to short- and long-term exposure to anthropogenic activities. Many life-history traits make it difficult to study these species in the wild. Stranding events provide invaluable data to infer key aspects of sea turtle ecology, such as regional occurrence, health status, mortality rates, and potential threats. In this study, we investigate spatial and temporal patterns of sea turtle occurrence and mortality in the southwestern Atlantic Ocean based on a systematic, large-scale survey programme covering 1040 km of coastline during 732 d of two consecutive years. From the 12 571 animals recovered, juvenile green turtles were the most common (90.4%), but four other species were also recorded. A significant non-linear effect of time and space in the number of stranding events was observed. The most parsimonious mixed linear model further indicated that stranding events were influenced by individual (sex, size), health (body condition, pathologies, decomposition), and oceanographic factors (chlorophyll-a, sea surface temperature, salinity, wave height). More than simply describing the spatiotemporal occurrence and aspects of sea turtles’ life history in an otherwise understudied area, this intensive field study provides evidence for relatively high mortality rates—an essential baseline information for guiding conservation efforts.
Simplified Approach to Incorporating Glycemic Response Within a Continuous Insulin Infusion Algorithm to Improve the Incidence of Hypoglycemia in a Single Burn Center
Authors: Hendrix Hayden A , Velamuri Sai R , Sultan-Ali Ibrahim , Arif Faisal , Hickerson William L , Hill David M 
Published: April 2020

Abstract
Attaining adequate glycemic control in burn patients has been shown to reduce infection-related mortality. Previous internal evaluation of continuous insulin infusion (CII) use revealed a hypoglycemia rate of 0.6% and an average time within goal glycemic range (70–149 mg/dl) of 13.8 h/day. A new algorithm, designed to adjust dosage based on glycemic response, underwent six iterations over 2 years before the final version was implemented. The purpose of this retrospective study was to assess the post-implementation performance of the newly developed CII algorithm. The current study was powered to detect a 50% reduction in hypoglycemic events, as compared to a pre-implementation historical control. The cohort was 62% male with a mean age of 54.5 ± 17.4. Sixty-five percent had thermal injuries with a median 23.5 (11–45) %TBSA. There were no differences in demographics between groups. Among the 20 records reviewed, 5239 point-of-care glucose values were assessed. Post-implementation, hypoglycemia rates were significantly lower (0.6% vs 0.2%; P &lt; .001). There was no difference in median blood glucose between groups (149.9 vs 146.5 mg/dl; P = .56). Time spent within goal glycemic range was not significantly different (13.8 vs 14.7 h/day; P = 0.23). There were no differences in infection, length of stay, or survival. The consolidation, education, and implementation of a single, dynamic CII algorithm reduced the incidence of hypoglycemia. The authors expect that education and diligence with follow-up glucose monitoring will further improve time within goal glycemic range by preventing rebound hyperglycemia.
The Impact of Blood Type O on Major Outcomes in Patients With Severe Burns
Authors: Yao Renqi , Hou Wenjia , Shen Tuo , Zhao Shuo , He Xingfeng , Sun Yu , Ma Bing , Wu Guosheng , Xia Zhaofan 
Published: April 2020

Abstract
ABO blood type has been reported to be a predictor of poor prognosis in critically ill patients. Here, we aim to correlate different blood types with clinical outcomes in patients with severe burns. We conducted a single-center retrospective cohort study by enrolling patients with severe burn injuries (≥40% TBSA) between January 2012 and December 2017. Baseline characteristics and clinical outcomes were compared between disparate ABO blood types (type O vs non-O type). Multivariate logistic and linear regression analyses were performed to identify an association between ABO blood type and clinical outcomes, including in-hospital mortality, the development of acute kidney injury (AKI), and hospital or ICU length of stay. A total of 141 patients were finally enrolled in the current study. Mortality was significantly higher in patients with type O blood compared with those of other blood types. The development of AKI was significantly higher in patients with blood type O vs non-O blood type (P = .001). Multivariate analysis demonstrated that blood type O was independently associated with in-hospital mortality and AKI occurrence after adjusting for other potential confounders. Our findings indicated the blood type O was an independent risk factor of both increased mortality and the development of AKI postburn. More prudent and specific treatments are required in treating these patients to avoid poor prognosis.
Omitting surgery for early breast cancer showing clinical complete response to primary systemic therapy
Authors: Shigematsu Hideo , Fujisawa Tomomi , Shien Tadahiko , Iwata Hiroji 
Published: April 2020

Abstract
Breast cancer is highly sensitive to systemic therapy. High probability of pathological complete response suggests a clinical question that omitting surgery is an effective alternative to surgery in breast cancer showing clinical complete response to primary systemic therapy. However, the validity of omitting surgery for early breast cancer after primary systemic therapy has not been sufficiently established; thus, even if pathological complete response is expected in patients showing clinical complete response, excision of the primary tumor site remains the standard treatment of breast cancer. Inappropriate omitting surgery increases the incidence of local recurrence, which can be the risk of a subsequent distant metastasis and reduced overall survival. To achieve acceptable local control rate, omitting surgery should be investigated in patients with early breast cancer where a high percentage of pathological complete response, a high concordance rate between clinical complete response and pathological complete response and an acceptable local control rate are expected. This review presents concept and ongoing clinical trials for omitting surgery for patients with breast cancer showing clinical complete response to primary systemic therapy.
Deltamethrin Persistence on Textiles Used to Make Visual Targets for Tsetse Fly Control is Positively Correlated With Their Polyester Content
Authors: Kamba Mebourou Emmanuel , Bourquin Martine , Oloo Francis , Vallat Armelle , Guerin Patrick M 
Published: April 2020

AbstractInsecticide-impregnated traps and visual targets are used for tsetse (Diptera, Glossinidae) population control. Such devices are made with textiles and deltamethrin is frequently the insecticide of choice. However, persistence of an insecticide on textiles is affected by exposure to weather. Here we examine the effect of weathering on the capacity of four textiles with increasing proportions of polyester (0, 35, 65, and 100%) with cotton and viscose to retain deltamethrin. Textiles tested were those used to make visual targets in a pan-African program to maximize target efficiency for controlling tsetse vectors of African trypanosomiasis. Following impregnation in an aqueous suspension of deltamethrin at 1,000 mg/m2, textiles were weathered for 18 mo at Lambwe Valley, Kenya and sampled every 3 mo to make knockdown tests on the tsetse fly Glossina pallidipes Austen. Deltamethrin content of the textiles was established using gas chromatography mass-spectrometry at impregnation and after 9 mo of weathering. Textiles with higher proportions of polyester retained deltamethrin better: respectively, 100% polyester and 65:35 polyester/viscose textiles retained deltamethrin at 17 and 11 mg/m2 9-mo post-treatment that caused 100% knockdown in G. pallidipes after 1 h, and killed 67 and 47% of flies, respectively, after 24 h. Eighteen-month weathered 100% polyester treated textile still knocked down all tsetse exposed to it within 2 h. The LD50 for deltamethrin on filter paper for G. pallidipes was estimated at 28.8 mg/m2, indicating that deltamethrin is more available on polyester to kill tsetse.