bluuebunny/crossref_metadata_2025_split huggingface dataset:
A population pharmacokinetic model is beneficial in quantifying hair concentrations of ritonavir-boosted atazanavir: A study of HIV-infected Zimbabwean adolescents.
Authors: NGARA Bernard , Zvada Simbarashe , Chawana Tariro Dianah , Stray-Pedersen Babill , Nhachi Charles Fungai Brian , Rusakaniko Simbarashe 
Published: June 2020

Abstract
Background Adolescents experience higher levels of non-adherence to HIV treatment. Drug concentration in hair promises to be reliable for assessing exposure to antiretroviral (ARV) drugs. Pharmacokinetic modelling can explore utility of drug in hair. We aimed at developing and validating a pharmacokinetic model based on atazanavir/ritonavir (ATV/r) in hair and identify factors associated with variabilities in hair accumulation. Methods We based the study on secondary data analysis whereby data from a previous study on Zimbabwean adolescents which collected hair samples at enrolment and three months follow-up was used in model development. We performed model development in NONMEM (version 7.3) ADVAN 13. Results There is 16% / 18% of the respective ATV/r in hair as a ratio of steady-state trough plasma concentrations. At follow-up, we estimated an increase of 30% /42% of respective ATV/r in hair. We associated a unit increase in adherence score with 2% increase in hair concentration both ATV/r. Thinner participants had 54% higher while overweight had 21% lower atazanavir in hair compared to normal weight participants. Adolescents receiving care from fellow siblings had atazanavir in hair at least 54% less compared to other forms of care. Conclusion The determinants of increased ATV/r concentrations in hair found in our analysis are monitoring at follow up event, body mass index, and caregiver status. Measuring drug concentration in hair is feasibly accomplished and could be more accurate for monitoring ARV drugs exposure. Keywords Pharmacokinetic modelling, HIV/AIDS, Adolescents, Adherence, Hair, NONMEM
Occupational pesticide exposure and the risk of death in patients with Parkinson’s disease: an observational study in Southern Brazil.
Authors: Medeiros Márcio Schneider , Reddy Sumanth P. , Socal Mariana P. , Schumacher-Schuh Artur Francisco , Rieder Carlos Roberto de Mello 
Published: June 2020

Abstract
Background: Multiple studies have suggested that various pesticides are associated with a higher risk of developing Parkinson’s disease (PD) and may influence the progression of the disease. However, the evidence regarding the impact of pesticide exposure on mortality among patients with PD is equivocal. This study examines whether pesticide exposure influences the risk of mortality among patients with PD in Southern Brazil. Methods: A total of 150 patients with idiopathic PD were enrolled from 2008-2013 and followed until 2019. In addition to undergoing a detailed neurologic evaluation, patients completed surveys regarding socioeconomic status and environmental exposures. Results: Twenty patients (13.3%) reported a history of occupational pesticide exposure with a median duration of exposure of 10 years (mean = 13.1, SD = 11.2). Patients with a history of occupational pesticide exposure had higher UPDRS-III scores, though there were no significant differences in regards to age, sex, disease duration, Charlson Comorbidity Index, and age at symptom onset. Patients with occupational pesticide exposure were more than twice as likely to die than their unexposed PD counterparts (HR = 2.32, 95% CI [1.15, 4.66], p = 0.018). Occupational pesticide exposure was also a significant predictor of death in a cox-proportional hazards model which included smoking and caffeine intake history (HR = 2.23, 95% CI [1.09, 4.59], p = 0.03)) and another which included several measures of socioeconomic status (HR = 3.91, 95% CI [1.32, 11.58], p = 0.01).Conclusion: In this prospective cohort study, we found an increased all-cause mortality risk in PD patients with occupational exposure to pesticides. More studies are needed to further analyze this topic with longer follow-up periods, more detailed exposure information, and more specific causes of mortality.
CT findings of patients infected with SARS-CoV-2
Authors: Wang Xiaoyang , Liu Chenbin , Hong Liang , Yuan Cuiyun , Ding Jiguang , Jia Qing , Sun Gangqiang , Peng Wenxian , Sun Qingfeng 
Published: June 2020

Abstract
Objective: We aimed to describe the chest CT findings in sixty-seven patients infected by Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Method and material: We retrospectively reviewed 67 patients hospitalized in Ruian People's Hospital. All the patients received the positive diagnosis of SARS-CoV-2 infection. The CT and clinical data were collected between January 23 rd , 2020 and February 10 th , 2020. The CT images were analyzed by the senior radiologists. Conclusion: There are 54 patients with positive CT findings and 13 patients with negative CT findings. The typical CT findings in hospitalized patients with SARS-CoV-2 infection were ground glass opacities (42/54), lesions located in the peripheral area (50/54), multiple lesions (46/54), and lesions located in the lower lobes (42/54). There were less typical CT findings, including air bronchogram (18/54), pleural thickening or pleural effusion (14/54), consolidation (12/54), lesions in the upper lobes (12/54), interlobular septal thickening (11/54), reversed halo sign (9/54), single lesion (8/54), air cavities (4/54), bronchial wall thickening (3/54), and intrathoracic lymph node enlargement (2/54).
miR-138-5p inhibits the metastasis of prostate cancer by targeting FOXC1
Authors: Zhang Dapeng , Liu Xiaodong , Zhang Qingwei , Chen Xin 
Published: June 2020

Abstract
Background: This study aimed to uncover the effect of miR-138-5p on the proliferation and metastasis of PCa cell lines, and further explore the potential regulatory mechanisms via regulating FOXC1.Methods: 60 pairs tumor tissues and corresponding paracancerous tissues from PCa patients were collected to determine the expression level of miR-138-5p by qRT-PCR. Subsequently, over-expression of miR-138-5p were established to explore the proliferation and metastasis of miR-138-5p in PCa cell lines was analyzed by CCK-8, Tranwell assay and Wounding healing assay, respectively. Bioinformatics analysis and luciferase reporter gene assay were performed to search for the target genes of miR-138-5p, and FOXC1 was selected. Finally, the biological role of miR-138-5p and FOXC1 in the progression of PCa was clarified by a series of rescue experiments. Results: The results of qRT-PCR revealed that miR-138-5p was lowly expressed in PCa tissues and cell lines. Besdies, these PCa patients with low-miR-138-5p had a higher Gleason score, lymph node metastasis and poor prognosis of PCa, compared with the patients with high-miR-138-5p. Over-expression of miR-138-5p inhibited the viability, migratory and invasive capacities of PC-3 and DU-145 cells. Bioinformatics analysis and luciferase reporter gene assay suggested that FOXC1 was predicted to be the target of miR-138-5p. Moreover, FOXC1 level was negatively correlated to that of miR-138-5p in PCa tissues. Importantly, FOXC1 could reverse miR-138-5p mimic induced-inhibition of PCa malignant progression.Conclusions: Downregulated miR-138-5p was closely associated with Gleason score, distant metastasis and poor prognosis of PCa patients. In addition, miR-138-5p alleviated the malignant progression of PCa by targeting and downregulating FOXC1.
Surgical ligation of patent ductus arteriosus in preterm neonates weighing less than 1,500 g: A 9-year single center experience
Authors: Lee Jun Ho , Lee Hyun Ju , Park Hyun-Kyung , Ahn Ja-Hye , Kim Hee Sun , Jang Hyo Jun , Ro Sun Kyun , Kim Hyuck 
Published: June 2020

Abstract
Background: The aim of this study was to determine the feasibility and outcomes of early surgical ligation in preterm neonates with hemodynamically significant patent ductus arteriosus (HSPDA) and to investigate predictors for surgical treatment after unsuccessful medical management.Methods: Medical records from the neonatal intensive care unit of Hanyang University Seoul Hospital from January 2010 to December 2018 were retrospectively reviewed. 233 preterm neonates weighing less than 1,500 g with HSPDA were enrolled in our study. Of these preterm neonates, 134 underwent surgical ligation and were subdivided into the early ligation group (n = 49; within 10 days of age) and the late ligation group (n = 85; after 10 days of age).Results: The mean gestational age and birth weight were significantly lower in the patent ductus arteriosus (PDA) ligation group than in the Non-ligation group (p < 0.001). PDA ductal diameter > 2.0 mm (p < 0.001), low Apgar score at 5 minutes (p = 0.033), and chorioamnionitis (p = 0.037) were the predictors for receiving surgical treatment for PDA. Early ligation was significantly associated with a low incidence of culture-proven sepsis (p = 0.004), mechanical ventilator time > 4 weeks (p = 0.007), necrotizing enterocolitis stage (NEC) ≥ III (p = 0.022), and intraventricular hemorrhage (IVH) grade ≥ III (p = 0.035).Conclusions: Early surgical ligation minimizes the adverse effects of HSPDA in predicted preterm neonates who subsequently require surgical treatment for PDA. This result suggests that in preterm neonates weighing less than 1,500 g with HSPDA that is unresponsive to medical treatment, delayed ductal closure should be avoided to reduce severe NEC, severe IVH, culture-proven sepsis, and facilitate earlier endotracheal extubation.
Experiential learning for children's dental anxiety: a cluster randomized trial
Authors: Zhu Minmin , Yu Hongbing , Xie Bo , Li Hongwen , He Qian , Li Huimin , Su Jing , Li Xueqi 
Published: June 2020

Abstract
Background: Dental anxiety (DA) has an impact on the quality of dental treatment and may have long-lasting implications for children. A recent study introducing experiential learning (EL) into children’s oral health promotion resulted in better oral hygiene. The purpose of the study is to evaluate whether EL can reduce children's DA. Methods: In September 2018, we recruited 988 children aged 7-8 years from 24 classes to participate in a cluster-randomized trial. Classes were randomly assigned to EL (in which children received a lively presentation on oral health and participated in a role play in a simulated dental clinic in the classroom) or the Tell-Show-Do (TSD) group (in which children received a conventional TSD behavior management). The primary outcome was the prevalence of DA after the procedure of pit and fissure sealant (PFS), assessed by a modified children's fear survey schedule-dental subscale. Secondary outcomes were changes in blood pressures (BP) and pulse rates (PR) before and after the PFS procedure. The intervention effects were estimated by means of mixed effect models, which included covariates of gender and school (and baseline value for BP and PR only), and a random cluster effect.Results: In 396 children of the EL group who received the PFS treatment, the prevalence of DA (score ≥ 38) was 18.5%, compared with 24.3% in 391 children of the TSD group (OR=0.65; 95% confidence interval, 0.46-0.93; P=0.019). The increases in BP and PR after the PFS were also significantly less in the EL group. Conclusions: School-based experiential learning intervention before a dental visit is feasible and effective in lowering children’s dental anxiety during PFS.Trial registration: The trial was registered in Chinese Clinical Trial Registry on 5 January 2020 (No.: ChiCTR2000028878, retrospectively registered), http://www.chictr.org.cn/edit.aspx?pid=47970&htm=4.
Contextual Effects of Social Integration and Disintegration on Health Status: Evidence from South Korea
Authors: Jo Eun-Bi , Kwon Rang Hee , Jung Minsoo 
Published: June 2020

Abstract
Background : Many studies have shown that various social integration variables represented by social capital are beneficial to communities, including collective health. However, the rapid decline in fertility rates and the breakup of familyism in developed countries require a new approach to social disintegration, but the literature is insufficient. Here, we explored the contextual effects of social integration and social disintegration on the health of individuals.

Methods : The research data consist of merged datasets of 6909 respondents who were quota-sampled by approximately 30 people from 229 local governments in Korea. The individual-level independent variable is a social integration measure consisting of 26 questions in four areas. The community-level independent variables are five integral and aggregate variables extracted from 81 indicators. The dependent variable is self-rated health status. Potential confounders are gender, age, annual income, educational attainment, district type, and the number of beds in medical institutions per 1,000 people.

Results : The results showed that at the individual level, the higher the inclusive attitude of in- and out-of-networks, after adjusting for potential confounders, the less likely the respondent belongs to the unhealthy group (p <0.001). At the community level, the higher the proportion of single-person households in a community after adjusting for potential confounders, the less likely the respondent belongs to the unhealthy group (p <0.05). The effect size was 0.22.

Conclusion : Thus far, social integration has been preferred, with the positive aspects of social capital being emphasized. However, this study shows that in some cases, social disintegration can instead positively influence an individual's health. Therefore, further studies of the various conditions of social context effects on health are necessary.
Cyclin H predicts a poor prognosis and promotes the proliferation in ovarian cancer
Authors: Peng Chen , Yang Yansong , Ji Li , Yang Panpan , Yang Xiaoqing , Zhang Yuquan 
Published: June 2020

Abstract
Background: Cell cycle dysregulation plays a key role in the pathogenesis of malignant tumor. As a part of CDK-activating kinase (CAK) trimeric complex, Cyclin H is necessary for the regulation of the cell cycle and proliferation. The objective of this investigation was to characterize the clinical significance and biological functions of cyclin H in ovarian cancer.Methods: Immunohistochemical staining was performed on 60 cases of ovarian cancer and the correlation between cyclin H expression and clinical characteristics was analyzed. The function of cyclin H in ovarian cancers was further explored using HO8910 cells and subcutaneous xenograft model of nude mice. Results: Cyclin H was slightly expressed in grade 1 ovarian cancer and highly expressed in high grade 2 and grade 3 tissues. The result of spearman rank correlation analysis showed that the expression of cyclin H positively correlated with tumor grade, the FIGO stage, histologic grade and peritoneal metastasis of ovarian cancer. Cyclin H was positively correlates with Ki67 and p-CDK2 in ovarian cancer. In addition, we found the five-year survival rate of cyclin H low expressed patients was higher than that in the cyclin H high expressed patients. Furthermore, knockdown of cyclin H was then achieved using a shRNA in HO8910 ovarian cancer cell line. Silencing of cyclin H resulted in G1/S cell cycle arrest of ovarian cancer cells and suppressed the growth of ovarian cancer. The expression of Ki67 was decreased in cyclin H silenced ovarian cancer. Conclusions: These results suggest that high expression of cyclin H predicts a poor prognosis and promotes the growth of ovarian cancer via regulating cell cycle.
Non-active implantable device treating acid reflux with a new dynamic treatment approach: 1-year results
Authors: Bjelović Miloš , Harsányi László , Altorjay Áron , Kincses Zsolt , Forsell Peter 
Published: June 2020

Abstract
BACKGROUND RefluxStop™ is an implantable, non-active, single use device used in the laparoscopic treatment of GERD. RefluxStop™ aims to block the movement of the LES up into the thorax and keep the angle of His in its original, anatomically correct position. This new device restores normal anatomy, leaving the food passageway unaffected. METHODS In a prospective, single arm, multicentric clinical investigation analyzing safety and effectiveness of the RefluxStop™ device to treat GERD, 50 subjects with chronic GERD were operated using a standardized surgical technique between December 2016 and September 2017. They were followed up for one year (CE-mark investigation 6-months). Primary safety outcome was prevalence of serious adverse events related to the device, and primary effectiveness outcome reduction of GERD symptoms based on GERD-HRQL score. Secondary outcomes were prevalence of adverse events other than serious adverse events, reduction of total acid exposure time in 24-hour pH monitoring, and reduction in average daily PPI usage and subject satisfaction. RESULTS There were no serious adverse events related to the device. Average GERD-HRQL total score at 1 year improved 86% from baseline (p<0.001). 24-hour pH monitoring compared to baseline showed a mean reduction percentage of overall time with pH<4 from 16.35% to 0.80% at the 6-month visit (p<0.001), with 98% of subjects showing normal 24-hour pH. At 1 year: No new cases of dysphagia were recorded, present in 2 subjects, which existed already at baseline. Regular daily PPI usage occurred in all 50 subjects at baseline. At 1-year follow-up, only 1 subject took regular daily PPIs due to a too low placement of the device thereby prohibiting its function. None or minimal occasional episodes of regurgitation occurred in 97.8% of evaluable subjects. Gas bloating disappeared in 30 subjects and improved in 7 subjects.CONCLUSION The new principle of RefluxStop™ is safe and effective to treat GERD according to investigation results. At 1-year follow-up, both the GERD-HRQL score and 24-h pH monitoring results indicate success for the new treatment principle. In addition, with the dynamic treatment for acid reflux, which avoids compressing the food passageway, prevalence of dysphagia and gas bloating are significantly reduced.
Classification and morphology of middle mesial canals of mandibular first molars in a Southern Chinese subpopulation: a cone-beam computed tomographic study
Authors: Yang Yeqing , Chen Ming , Zeng Junkai , Wu Buling 
Published: June 2020

Abstract
Background: Cone-beam computed tomography (CBCT) was employed to study the morphology and curvature of middle mesial canals (MMCs) of mandibular first molars (MFMs).Methods: CBCT scanning was performed on MFMs of 1100 patients. Patients' images that met the inclusion criteria were divided into group A (<40 years old) and group B (≥40 years old) for further study. The images were used to study the incidence of MMCs at different ages, measure the curvature of MMCs in the mesiodistal and buccolingual directions using the Schneider method, and observe the anatomical morphology of the mesial root canal system.Results: In 875 patients, 1750 MFM images met the inclusion criteria. Among these cases, 158 MFMs contained an MMC, yielding an incidence rate of 9.03%. The incidence rate of MMCs was 11.22% in group A and 6.61% in group B, and this difference was statistically significant (P<0.05). The curvature in the mesiodistal direction was 29.39±8.53º in group A and 26.06±8.50º in group B, and this difference was also significant (P<0.05). The curved regions in groups A and B were often located in the middle 1/3 of canal. No significant difference in the distance between MMC orifices and mesiobuccal canal orifices or mesiolingual canal orifices was noted (P>0.05). The most common mesial root canal morphological type was type II (3-2) (53.80%). Conclusion: The incidence of MMCs in MFMs declined as age increased. The canal systems of MMCs were varied and complex, mainly exhibiting an obvious mesiodistal curve. CBCT is an outstanding method to help guide root canal therapy.
Transposable element abundance correlates with mode of transmission in microsporidian parasites
Authors: de Albuquerque Nathalia Rammé Medeiros , Ebert Dieter , Haag Karen Luisa 
Published: June 2020

Abstract
The extreme genome reduction and physiological simplicity of some microsporidia has been attributed to their intracellular, obligate parasitic lifestyle. Although not all microsporidian genomes are small (size range from about 2 to 50 MB), it is suggested that the size of their genomes has been streamlined by natural selection. We explore the hypothesis that vertical transmission in microsporidia produces population bottlenecks, and thus reduces the effectiveness of natural selection. Here we compare the transposable element (TE) content of 47 microsporidian genomes, and show that genome size is positively correlated with the amount of TEs, and that species that experience vertical transmission have larger genomes with higher proportion of TEs. Our findings are consistent with earlier studies inferring that nonadaptive processes play an important role in microsporidian evolution.
Electrochemical assessment of pigments-binding media interactions in oil paint deterioration: a case study on indigo and Prussian blue
Authors: Doménech-Carbó Antonio , Doménech-Carbó María Teresa , Osete-Cortina Laura , Donnici Margherita , Guasch-Ferré Nuria , Gasol-Fargas Rosa M. , Iglesias-Campos Manuel Ángel 
Published: June 2020

Abstract
The degradation of laboratory oil paint film specimens containing indigo and Prussian blue pigments and pictorial samples from the Sant Francesc de Paula painting exhibited in the Tomàs Balvey Arxiu Museum (Cardedeu (Catalonia), Spain) has been studied by voltammetry of immobilized particles. This technique, combined with light microscopy, scanning electron microscopy-energy dispersive X-ray analysis, nanoindentation-atomic force microscopy, attenuated total reflectance-Fourier-transform infrared spectroscopy and gas chromatography-mass spectroscopy techniques permits one to propose a dual scheme for the degradation of the pigments when naturally aged and submitted to accelerated UVA aging. Under conditions of moderate temperature, humidity and natural illumination, and low gradients of these parameters, Prussian blue acts as a radical scavenger moderating the production of reactive oxygen species produced in the oil binding medium by the action of ultraviolet radiation, resulting in the formation, in the solid state, of the solid-solution, {KFeIII[FeII(CN)6]}x{FeIII[FeIII(CN)6]}1–x, known as Berlin green, which then promotes the formation of indigo adducts with radicals. In the case of localized areas of the Sant Francesc de Paula paint, strong degradation occurs where Prussian blue acts as a promoter of the indigo oxidation to isatin, thus resulting in a considerable chromatic shift.