bluuebunny/crossref_metadata_2025_split huggingface dataset:
Long non-coding RNA LINC00115 contributes to the progression of colorectal cancer by targeting miR-489-3p via PI3K/AKT/mTOR pathway
Authors: Feng Weiyu , Li Baodong , Wang Jinbang , Zhang Huiliang , Liu Yonggang , Xu Dongli , Cheng Ke , Zhuang Jing 
Published: July 2020

Abstract
Background
Long noncoding RNAs (lncRNAs) are tumor-related regulators and have been found to be involved in the underlying molecular mechanisms of colorectal cancer (CRC). However, the role of lncRNA LINC00115 during CRC progression is not entirely elucidated.
Methods
The expression of LINC00115 was analyzed in paired CRC tissue samples and its clinical significance was evaluated. The biological effects on CRC cells proliferation, apoptosis, migration, invasion and PI3K/AKT/mTOR signaling were assessed by Cell Counting Kit-8 assay, Transwell assay, flow cytometry analysis and Western blot, respectively. The regulatory relationship between LINC00115 and miR-489-3p was determined by dual-luciferase reporter assays.
Results
LINC00115 was significantly overexpressed in CRC and its overexpression predicted poor outcome of the patients. Downregulation of LINC00115 markedly inhibited CRC cell proliferation, increased cell apoptosis, and suppressed cell migration and invasion. Moreover, downregulation of LINC00115 led to the inactivation of PI3K/AKT/mTOR signaling. Bioinformatics analysis identified miR-489-3p as a candidate target of LINC00115. Furthermore, we revealed an inverse correlation between LINC00115 and miR-489-3p in CRC tissues. miR-489-3p might directly target LINC00115 and downregulation of miR-489-3p could rescue the biological effects induced by the absence of LINC0015.
Conclusion
LINC00115 serves as an excellent oncogene of CRC metastasis, the deeper understanding of LINC00115/miR-489-3p axis might provide potential therapeutic targets for CRC metastasis.
The efficacy of mineral corticoid receptor antagonist, canrenone, in COVID-19 patients
Authors: Vicenzi Marco , Ruscica Massimiliano , Iodice Simona , Rota Irene , Ratti Angelo , Cosola Roberta Di , Bollati Valentina , Corsini Alberto , Aliberti Stefano , Blasi Francesco 
Published: July 2020

Abstract
Purpose: Despite the spread of SARS-CoV-2 global pandemic and the volume of clinical trials launched, no specific therapeutic drug approaches improving outcomes have been so far approved. In COVID-19 patients, aldosterone via ACE2 deregulation may be responsible for systemic and pulmonary vasoconstriction, inflammation and oxidative organ damage.Aim: To verify retrospectively the impact of the mineral corticoid receptor antagonist canrerone i.v. on the need of invasive ventilatory support and/or all-cause in-hospital mortality.Study design: In this retrospective study (CARDIOVID-19, ID-107162), COVID-19 patients hospitalized for severe respiratory failure were taken care by pneumologists or cardiologists and received two different therapeutic approaches according to personal skill of referral medical team on pharmacological management. Group 1 (n=39) were given vasodilator agents or RAAS-inhibitors and group 2 (n=30) were given canrenone i.v.Results: Among 69 consecutive COVID-19 patients, enrolled in COVID-19 NETWORK registry, the group given canrenone (200 mg/q.d. for a median of 14±11) showed a free event rate of 83% with a survival percentage of 90%. In group 1, not receiving canrenone, free event rate was 51% and survival 64%. Kaplan-Meier analysis for composite outcomes showed a Log Rank of 0.0004 and for mortality, Log Rank was 0.005.Conclusions: The novelty of our observation relies on the independent positive impact of the canrenone, a mineral corticoid receptor antagonist, on all-cause mortality and clinical improvement in COVID-19 patients.
Gut Microbiota May Underlie the Predisposition of Healthy Individuals to COVID-19-Sensitive Proteomic Biomarkers
Authors: Gou Wanglong , Fu Yuanqing , Yue Liang , Chen Geng-dong , Cai Xue , Shuai Menglei , Xu Fengzhe , Yi Xiao , Chen Hao , Zhu Yi , Xiao Mian-li , Jiang Zengliang , Miao Zelei , Xiao Congmei , Shen Bo , Wu Xiaomai , Zhao Haihong , Ling Wenhua , Wang Jun , Chen Yu-ming , Guo Tiannan , Zheng Ju-Sheng 
Published: July 2020

Abstract
Background: The COVID-19 pandemic is spreading globally with high disparity in the susceptibility of the disease severity. Identification of the key underlying factors for this disparity is highly warranted. Results: Here we describe constructing a proteomic risk score (PRS) based on 20 blood proteomic biomarkers which related to the progression to severe COVID-19. Among COVID-19 patients, per 10% increment in the PRS was associated with a 57% higher risk of progressing to clinically severe phase (RR=1.57; 95% CI, 1.35-1.82). We demonstrate that in our own cohort of 990 individuals without infection, this proteomic risk score is positively associated with proinflammatory cytokines mainly among older, but not younger, individuals. We further discovered that a core set of gut microbiota could accurately predict the blood proteomic biomarkers of COVID-19 using a machine learning model. The core OTU-predicted PRS had a significant correlation with actual PRS both cross-sectionally (n=132, p<0.001) and prospectively (n=169, p<0.05). Most of the core OTUs were highly correlated with proinflammatory cytokines. Fecal metabolomics analysis suggested potential amino acid-related pathways linking the above core gut microbiota to inflammation.Conclusions: Our study suggests that gut microbiota may underlie the predisposition of healthy individuals to COVID-19-sensitive proteomic biomarkers.
Comparison of Unilateral and Bilateral Polymethylmethacrylate-augmented Cannulated Pedicle Screw Fixation for Management of Lumbar Spondylolisthesis with Osteoporosis
Authors: Liu Yao-yao , Xiao Jun , Jin Huai-jian , Wang Zhong , Yin Xiang , Liu Ming-yong , Zhao Jian-hua , Liu Peng , Dai Fei 
Published: July 2020

Abstract
Background: Many studies have shown that cannulated pedicle screw (CPS) augmented by polymethylmethacrylate (PMMA) can obtain a satisfactory clinical efficacy in the treatment of lumbar spondylolisthesis with osteoporosis. However, accurate application of CPSs will help to avoid the difficulty of screw revision and reduce the incidence of PMMA related complications. This study aims to investigate the mid-term efficacy of CPS comparing unilateral and bilateral application in this common lumbar degenerative disease. Methods: Between May 2011 and May 2018, 50 patients with posterior fixation and fusion using traditional pedicle screw or CPSs for lumbar spondylolisthesis with osteoporosis were included in the study. Patients were divided into 2 groups: those with unilateral PMMA-augmented CPSs (group UC n=29) and those with bilateral PMMA-augmented CPSs (group BC, n=21). Operation time, blood loss, average hospitalization time, PMMA leakage and other complications were recorded. Visual Analog Scale (VAS) and Oswestry Disability Index (ODI) scores were used to evaluate the recovery of symptoms. Radiographic results were compared for the intervertebral fusion and screw loosening. Results: There were no significant differences in the baseline data of the 2 groups.VAS and ODI scores were improved significantly after surgery (P<0.05), with no differences between the groups (P>0.05). The operation time and blood loss in group UC were significantly lower than those in groups BC (P<0.05). However, the loss of intervertebral disc height and Taillard index had no difference in group UC compared with group BC. And the rates of PMMA leakage in group UC and BC were 7.0% and 11.9%, respectively (P<0.05). Bony fusion was achieved in all groups without screw loosening at the last follow-up. And only one patient experienced superficial infection in both groups, meanwhile two patients of cerebrospinal fluid leakage was observed in group BC.Conclusions: Unilateral application of PMMA-augmented CPS may provide adequate clinical safety and effectiveness in surgical treatment of lumbar spondylolisthesis with osteoporosis.
Explaining LARCs discontinuity in Ethiopia: The experience of women who access contraceptives in selected public health facilities
Authors: Abebe Solomon Mekonnen , Mihretie Getasew Sisay , Assefa Daniel T , Legesse Awol Yemane , Abera Mikyas 
Published: July 2020

Abstract
Background: Unintended pregnancy remains an important public health problem among teenagers and women in developing countries. Modern family planning methods, particularly Long Acting Reversible Contraceptives (LARCs), are highly effective in addressing the problem and its undesirable outcomes in maternal mortality. But dissatisfaction with contraceptives has contributed to their early discontinuation, but the reasons in Ethiopia are not adequately explored.Objective: This study aims to explore the main reasons of discontinuation of LARCs-use within one year of adoption in selected public health facilities in Ethiopia.Methods: Institution-based, multi-center prospective-cohort study design was used to identify the reasons of discontinuation among LARCs-users in Ethiopia. The study covered four university hospitals and health centers in Addis Ababa, Gondar, Mekelle and Jimma. The main study employed survey and in-depth interviews. But this article is based on qualitative data collected through in-depth interviews. It explored the decision-making processes involved in LARCs-discontinuation based on the experiences of 29 women aged 15-49 who did within a year of insertion. Interview data were thematically organized, coded and analyzed in light of the study objectives.Results: The study found women were sufficiently aware of the various types of contraceptives, including LARCs. Many of them experimented with different types before deciding on their choice. On LARCs, most were satisfied with its benefits while they also mentioned several reasons to discontinue its use including weight gain/loss, heavy menses, tiredness, reduced libido, desire to conceive and husbands’ disapproval. Their decisions to use or not use LARCs reflected the common cultural values about childbirth and myths on contraceptive use.Conclusions: The study found women use (or discontinue use) of contraceptives depends on their knowledge about their options, personal experience with side-effects, and the support from significant others, mainly husbands. As such, any campaign to promote family planning methods has to take into account the status of women in the Ethiopian society and cultural definition of their sexuality.
Non-hormonal Treatments For Menopausal Symptoms and Sleep Disturbances: A Comparison Between Purified Pollen Extracts and Soy Isoflavones
Authors: De Franciscis Pasquale , Conte Anna , Schiattarella Antonio , Riemma Gaetano , Cobellis Luigi , Colacurci Nicola 
Published: July 2020

Background:
Besides hot-flushes, sleep disturbances increase around menopause, impacting on the
quality of life. When hormone replacement therapy is contraindicated, it is necessary to provide alternative treatments.

Objectives:
This study aimed to observe the effects of an herbal remedy from pollen extracts and soy isoflavones
for menopausal complaints, particularly on sleep disorders.

Methods:
A six-month prospective observational study was performed in women in natural menopause suffering
from menopausal symptoms and sleep disturbances. Three groups were compared: 57 women receiving two tablets/
day containing herbal remedy from pollen extracts (group A), 60 women receiving one tablet/day containing
isoflavones 60 mg (group B), 47 women not receiving any treatment (group C). At 3 (T3) and 6 months (T6), the
daily number of hot-flushes, Kupperman index for menopausal symptoms, the Pittsburgh Sleep Quality Index
(PSQI) test were assessed.

Results:
Both groups A and B showed a significant improvement of hot flushes (p<0.001) and Kuppermann Index
(p<0.001) from T0 to T3 and from T0 to T6. No significant differences between treatment groups were found
at T3, while at T6 group A showed greater decrease of daily hot flashes and better improvement of Kupperman
Index as compared to group B (respectively, -48.8% versus -18.4% and -24.4% versus -15.4%; p<0.001). Improvement
of global sleep quality was more evident in the pollen treated group compared to isoflavones group at
both three (-24.7% versus -9.3%, p<0.001) and six (-52.9% vs -4.0%; p<0.001) months, mainly for the scores
related to subjective sleep quality, sleep latency and habitual sleep efficiency.

Conclusions:
Non-hormonal treatments can effectively be used in symptomatic menopausal women: among these,
after six months of treatment, pollen extracts might achieve a better improvement of hot flushes, sleep disturbances
and menopause-related symptoms than soy isoflavones. Herbal remedy from pollen extracts is mainly
effective when the quality of sleep is the most disturbing complaint.
Atrial Remodeling in Atrial Fibrillation. Comorbidities and Markers of Disease Progression Predict Catheter Ablation Outcome
Authors: Szilágyi Judit , Sághy László 
Published: July 2020

:
Atrial fibrillation is the most common supraventricular arrhythmia affecting an increasing
proportion of the population in which mainstream therapy, i.e. catheter ablation, provides freedom
from arrhythmia in only a limited number of patients. Understanding the mechanism is key in
order to find more effective therapies and to improve patient selection. In this review, the structural
and electrophysiological changes of the atrial musculature that constitute atrial remodeling in atrial
fibrillaton and how risk factors and markers of disease progression can predict catheter ablation
outcome will be discussed in detail.
Gonioprobe, an Innovative Gamma-probe to Guide Parathyroid Radioguided Surgery: First Clinical Experiences with Navigator and Lock-ontarget Functions
Authors: Bononi Marco , Viviana Frantellizzi , De Feo Maria Silvia , Sollaku Saadi , Pani Arianna , Falconi Rita , Pani Roberto , Cavallaro Giuseppe , Brozzetti Stefania , De Vincentis Giuseppe 
Published: July 2020

Background:
Radioguided surgery represents a validated technique for the detection
and the excision of abnormal parathyroid glands responsible for primary hyperparathyroidism
(PHPT). To date little attention has been paid as to how the characteristics of gamma-probes can influence
surgical procedure and time, thus having an impact on postoperative morbidity, hospitalization
and costs.

Methods:
We designed a new prototype of gamma-probe, the Gonioprobe, and tested its clinical
utility in the operating room. Gonioprobe, thanks to its 5 scintillating independent crystals, performs
the dual function of Navigator and Lock-on-target. These characteristics allow the immediate
guidance of the surgeon’s hand towards the source with very high precision, and with a much
higher spatial resolution than commercial probes. Gonioprobe was used during intervention to detect
abnormal parathyroid tissue, and to ensure no radioactivity in surgery bed after adenoma removal.

Results:
We tested our gamma-probe on parathyroid adenomas particularly difficult to identify at a
visual inspection due to anatomy modifications from previous neck surgery and/or characterized by
uncommon localization. Moreover, parathyroid adenomas were hardly removable due to the
proximity to the esophagus, neck vessels and/or recurrent laryngeal nerve (RLN). An intraoperative
nerve monitoring system was used to protect the recurrent laryngeal nerve from injuries.
Parathyroid hormone (PTH) assay and frozen biopsy confirmed the successful excision of the adenomas.

Conclusion:
The intraoperative use of the innovative Gonioprobe along with the nerve monitoring system allowed an
accurate and safe removal of parathyroid adenomas and offered a significant advantage by reducing surgical time and
postoperative complications, as well as hospitalization and costs.
Purifying Crude Petroleum by Using Porous Ceramic Balls
Authors: Hadi Enas M. , Yousif Khulood H. 
Published: July 2020

Background:
Background: In this study ceramic crude petroleum filter was prepared from Iraqi White Kaolin with ratio
(70%) and Alumina (Al2O3) with ratio (30%), with natural additives Palm Frond with ratios (5,10,15,25,35 and 45)% in
different partical size to produce pores, formed by dry pressing then fairing at 1100(˚C). The filters are harmless and
environmentally friendly materials. Some assessments were carried out, such as (apparent porosity ratio, water absorption
ratio, and apparent density). From the test results obtained the apparent porosity was 60.7% , water absorption was 89.3%
and an apparent density of 0.68% with a 45% ratio of fine (P.F).

Methods:
Size and distribution of pores were
characterized by using Scanning Electron Microscope (SEM). The crude petroleum treated with filters evaluated by tests
such as (API Gravity, Sulfur Content, Asphaltenes Content, and Metallic Content).

Results:
The result of API Gravity
before immersion crude petroleum filter balls was 24.70 and after immersion crude petroleum filter balls for 7 days for 30
% (P.F) increase to 31.0 and reach to 32.5 after immersion for 14 days. Sulfur Content before immersion crude petroleum
filter balls was 3.76 and after immersion crude petroleum filter balls for 7 days for 30 % (P.F) decrease down to 3.1 and
reach to 2.6 after immersion for 14 days.

Conclusion:
So Asphaltenes content before immersion crude petroleum filter
balls was 6.68 and after immersion crude petroleum filter balls for 7 days 30 % (P.F) decreased down to 2 and reach to 1.6
after immersion for 14 days, metallic contact such as Vanadium and Nickel before immersion crude petroleum filter balls
respectively was 86 ppm, 32 ppm while after immersion crude petroleum filter balls for 7 days they become 53.26 ppm
and 15.35 ppm and for 14 days they reached to 47.52 ppm and 11.43 ppm respectively.
EVALUASI PEMANFAATAN LABORATORIUM DALAM PEMBELAJARAN BIOLOGI DI MADRASAH ALIYAH NEGERI SEKABUPATEN SLEMAN
Authors: Sundari Retna 
Published: July 2020

Penelitian ini bertujuan mengevaluasi pemanfaatan laboratorium pembelajaran biologi di Madrasah Aliyah Negeri se-Kabupaten Sleman. Penelitian ini merupakan penelitian evaluasi model CIPP (Contex, Input, Process, Product). Subjek penelitian meliputi 5 Kepala Madrasah, 12 guru biologi dan 400 siswa kelas X di Madrasah Aliyah Negeri se-Kabupaten Sleman. Data dikumpulkan dengan angket, observasi dan wawancara terstruktur. Hasil penelitian menunjukkan (1) dukungan kepala madrasah terhadap program kegiatan laboratorium cukup tinggi, (2) ketersediaan sarana prasarana dalam kategori cukup, (3) pengelolaan laboratorium dalam kategori cukup, (4) pemanfaatan laboratorium dalam kategori cukup, (5) kendala-kendala yang dihadapi guru cukup tinggi, (6) minat siswa terhadap kegiatan laboratorium dan manfaat kegiatan laboratorium bagi siswa pada kategori tinggi. Kata kunci: evaluasi, pemanfaatan laboratorium, pembelajaran biologi
KEMAMPUAN MUSIKALITAS SEBAGAI SARANA PENGEMBANGAN KETERAMPILAN SOSIAL
Authors: Djohan Djohan 
Published: July 2020

Penelitian ini bertujuan untuk mengembangkan instrumen kepekaan musikalitas sehingga dapat memberikan kontribusi terhadap perkembangan peran musik dalam pendidikan serta peningkatan keterampilan sosial pada siswa sekolah dasar. Penelitian ini dilakukan terhadap 381 siswa kelas 3, 4, dan 5 sekolah dasar di Jakarta dan Yogyakarta. Hasil penelitian menunjukkan bahwa kepekaan musikalitas siswa dapat diukur melalui instrumen kepekaan terhadap musik dan kemampuan ini memiliki korelasi yang signifikan dengan skor kecerdasan sosial. Kata kunci: penghargaan diri, musik pendidikan, penilaian kemampuan musikalitas
Comparison of Geographic Information System and Subjective Assessments of Momentary Food Environments as Predictors of Food Intake: An Ecological Momentary Assessment Study
Authors: Elliston Katherine G , Schüz Benjamin , Albion Tim , Ferguson Stuart G 
Published: July 2020

Background
It has been observed that eating is influenced by the presence and availability of food. Being aware of the presence of food in the environment may enable mobile health (mHealth) apps to use geofencing techniques to determine the most appropriate time to proactively deliver interventions. To date, however, studies on eating typically rely on self-reports of environmental contexts, which may not be accurate or feasible for issuing mHealth interventions.

Objective
This study aimed to compare the subjective and geographic information system (GIS) assessments of the momentary food environment to explore the feasibility of using GIS data to predict eating behavior and inform geofenced interventions.

Methods
In total, 72 participants recorded their food intake in real-time for 14 days using an ecological momentary assessment approach. Participants logged their food intake and responded to approximately 5 randomly timed assessments each day. During each assessment, the participants reported the number and type of food outlets nearby. Their electronic diaries simultaneously recorded their GPS coordinates. The GPS data were later overlaid with a GIS map of food outlets to produce an objective count of the number of food outlets within 50 m of the participant.

Results
Correlations between self-reported and GIS counts of food outlets within 50 m were only of a small size (r=0.17; P<.001). Logistic regression analyses revealed that the GIS count significantly predicted eating similar to the self-reported counts (area under the curve for the receiver operating characteristic curve [AUC-ROC] self-report=0.53, SE 0.00 versus AUC-ROC 50 m GIS=0.53, SE 0.00; P=.41). However, there was a significant difference between the GIS-derived and self-reported counts of food outlets and the self-reported type of food outlets (AUC-ROC self-reported outlet type=0.56, SE 0.01; P<.001).

Conclusions
The subjective food environment appears to predict eating better than objectively measured food environments via GIS. mHealth apps may need to consider the type of food outlets rather than the raw number of outlets in an individual’s environment.