bluuebunny/crossref_metadata_2025_split huggingface dataset:
From space to lithosphere: inversion of the GOCE gravity gradients. Supply to the Earth’s interior study
Authors: Plasman Matthieu , Tiberi Christel , Cadio Cecilia , Saraswati Anita Thea , Pajot-Métivier Gwendoline , Diament Michel 
Published: June 2020

SUMMARY
The emergence of high resolution satellite measurements of the gravitational field (GOCE mission) offers promising perspectives for the study of the Earth’s interior. These new data call for the development of innovant analysis and interpretation methods. Here we combine a forward prism computation with a Bayesian resolution approach to invert for these gravity gradient data configuration. We apply and test our new method on satellite data configuration, that is 225 km height with a global and homogeneous geographic distribution. We first quantify the resolution of our method according to both data and parametrization characteristics. It appears that for reasonable density contrast values (0.1 g cm−3) crustal structures have to be wider than ∼28 km to be detectable in the GOCE signal. Deeper bodies are distinguishable for greater size (35 km size at 50 km depth, ∼80 km at 300 km depth). We invert the six tensor components, among which five are independent. By carefully testing each of them and their different combinations, we enlighten a trade off between the recovery of data and the sensitivity to inversion parameters. We particularly discussed this characteristic in terms of geometry of the synthetic model tested (structures orientation, 3-D geometry, etc.). In terms of RMS value, each component is always better explained if inverted solely, but the result is strongly affected by the inversion parametrization (smoothing, variances, etc.). On the contrary, the simultaneous inversion of several components displays a significant improvement for the global tensor recovery, more dependent on data than on density variance or on smoothness control. Comparing gravity and gradient inversions, we highlight the superiority of the GG data to better reproduce the structures especially in terms of vertical location. We successfully test our method on a realistic case of a complex subduction case for both gradient and gravity data. While the imaging of small crustal structures requires terrestrial gravity data set, the longest wavelength of the slab is well recovered with both data sets. The precision and homogeneous coverage of GOCE data however, counterbalance the heterogeneous and often quite non-existence coverage of terrestrial gravity data. This is particularly true in large areas which requires a coherent assemblage of heterogeneous data sets, or in high relief, vegetally covered and offshore zones.
Serum biomarkers associated with baseline clinical severity in young steroid-naïve Duchenne muscular dystrophy boys
Authors: Dang Utkarsh J , Ziemba Michael , Clemens Paula R , Hathout Yetrib , Conklin Laurie S ,   CINRG Vamorolone 002/003 Investigators, Hoffman Eric P 
Published: June 2020

AbstractDuchenne muscular dystrophy (DMD) is caused by loss of dystrophin in muscle, and while all patients share the primary gene and biochemical defect, there is considerable patient–patient variability in clinical symptoms. We sought to develop multivariate models of serum protein biomarkers that explained observed variation, using functional outcome measures as proxies for severity. Serum samples from 39 steroid-naïve DMD boys 4 to &lt;7 years enrolled into a clinical trial of vamorolone were studied (NCT02760264). Four assessments of gross motor function were carried out for each participant over a 6-week interval, and their mean was used as response for biomarker models. Weighted correlation network analysis was used for unsupervised clustering of 1305 proteins quantified using SOMAscan® aptamer profiling to define highly representative and connected proteins. Multivariate models of biomarkers were obtained for time to stand performance (strength phenotype; 17 proteins) and 6 min walk performance (endurance phenotype; 17 proteins) including some shared proteins. Identified proteins were tested with associations of mRNA expression with histological severity of muscle from dystrophinopathy patients (n = 28) and normal controls (n = 6). Strong associations predictive of both clinical and histological severity were found for ERBB4 (reductions in both blood and muscle with increasing severity), SOD1 (reductions in muscle and increases in blood with increasing severity) and CNTF (decreased levels in blood and muscle with increasing severity). We show that performance of DMD boys was effectively modeled with serum proteins, proximal strength associated with growth and remodeling pathways and muscle endurance centered on TGFβ and fibrosis pathways in muscle.
From the American Epicenter: Coronavirus Disease 2019 in Patients with Inflammatory Bowel Disease in the New York City Metropolitan Area
Authors: Axelrad Jordan E , Malter Lisa , Hong Simon , Chang Shannon , Bosworth Brian , Hudesman David 
Published: June 2020

Abstract

Background
We aimed to characterize patients with inflammatory bowel disease (IBD) and novel coronavirus disease 2019 (COVID-19).

Methods
We performed a case series of patients with IBD and confirmed or highly suspected COVID-19 to assess rates of severe outcomes.

Results
We identified 83 patients with IBD with confirmed (54%) or highly suspected (46%) COVID-19. The overall hospitalization rate was 6%, generally comprising patients with active Crohn’s disease or older men with comorbidities, and 1 patient expired.

Discussion
In this series of patients with IBD, severe outcomes of COVID-19 were rare and comparable to similarly aged individuals in the general population.
Linking Ecology, Morphology, and Behavior to Conservation: Lessons Learned from Studies of Sea Turtles
Authors: Wyneken Jeanette , Salmon Michael 
Published: June 2020

SynopsisHere we describe examples of studies that have contributed both to a basic understanding of the biology of imperiled marine turtles, and to their management and conservation. Key elements include, first and foremost, correctly identifying species that differ strikingly in their morphology at different life stages because with growth, they change size by several orders of magnitude and have accompanying shape changes. We also review comprehensive field studies documenting the need for management actions to correct abnormal shifts in sex ratios caused by climate change. We highlight the need to describe those perturbations in terms that are clear to regulators and personnel responsible for management and conservation policies. Finally, we review several basic studies that enhance our understanding of how selection has shaped morphological, functional, and performance attributes, and describe how that knowledge can be applied to the tasks required for enhancing species recovery.
Effect of childhood rotavirus vaccination on community rotavirus prevalence in rural Ecuador, 2008-13
Authors: Kraay Alicia N M , Ionides Edward L , Lee Gwenyth O , Trujillo William F Cevallos , Eisenberg Joseph N S 
Published: June 2020

Abstract

Background
Although live attenuated monovalent human rotavirus vaccine (Rotarix) efficacy has been characterized through randomized studies, its effectiveness, especially in non-clinical settings, is less clear. In this study, we estimate the impact of childhood Rotarix® vaccination on community rotavirus prevalence.

Methods
We analyse 10 years of serial population-based diarrhoea case-control study, which also included testing for rotavirus infection (n = 3430), and 29 months of all-cause diarrhoea active surveillance from a child cohort (n = 376) from rural Ecuador during a period in which Rotarix vaccination was introduced. We use weighted logistic regression from the case-control data to assess changes in community rotavirus prevalence (both symptomatic and asymptomatic) and all-cause diarrhoea after the vaccine was introduced. We also assess changes in all-cause diarrhoea rates in the child cohort (born 2008–13) using Cox regression, comparing time to first all-cause diarrhoea case by vaccine status.

Results
Overall, vaccine introduction among age-eligible children was associated with a 82.9% reduction [95% confidence interval (CI): 49.4%, 94.2%] in prevalence of rotavirus in participants without diarrhoea symptoms and a 46.0% reduction (95% CI: 6.2%, 68.9%) in prevalence of rotavirus infection among participants experiencing diarrhoea. Whereas all age groups benefited, this reduction was strongest among the youngest age groups. For young children, prevalence of symptomatic diarrhoea also decreased in the post-vaccine period in both the case-control study (reduction in prevalence for children &lt;1 year of age = 69.3%, 95% CI: 8.7%, 89.7%) and the cohort study (reduction in hazard for receipt of two Rotarix doses among children aged 0.5-2 years = 57.1%, 95% CI: 16.6, 77.9%).

Conclusions
Rotarix vaccination may suppress transmission, including asymptomatic transmission, in low- and middle-income settings. It was highly effective among children in a rural community setting and provides population-level benefits through indirect protection among adults.
Cognitive Heuristics, Inter-Institutional Politics, and Public Perceptions of Insulated Institutions: The Case of the U.S. Supreme Court
Authors: Zilis Michael A 
Published: June 2020

AbstractHow do citizens form perceptions about the ideological priorities of insulated institutions? Currently, there is little consensus on how or even whether citizens form such views. Focusing on the Supreme Court, I argue that perceptions of institutional ideology are influenced by an inter-institutional heuristic, or the popular perception that the president directly and indirectly influences the Court’s ideological direction. Using a multiple method approach, I demonstrate that citizens perceive the Court’s preferences to coincide with the president’s, changing predictably in the aggregate and varying substantially at the micro-level. The findings speak to debates about polarization in politics, showing that citizens may perceive insulated institutions as ideologically extreme due to factors beyond their control.
Mycobacterium avium Complex: Addressing Gaps in Diagnosis and Management
Authors: Daley Charles L , Winthrop Kevin L 
Published: June 2020

Abstract
Nontuberculous mycobacteria (NTM) are ubiquitous in the environment and an important cause of disease. The most common species causing pulmonary disease are members of Mycobacterium avium complex (MAC). MAC pulmonary disease (MAC-PD) can be chronic, debilitating, costly, and associated with a high mortality. However, MAC diagnoses are often delayed due to the nonspecific presentation of MAC-PD and radiological findings that overlap with other pulmonary diseases. Patients with risk factors and who meet the diagnostic criteria—which include clinical, radiological, and microbiologic criteria—should be considered for treatment. Diagnosis requires 2 or more positive sputum cultures or 1 bronchoscopic specimen culture. The recommendation for those who are treated is a 3-drug regimen including macrolide, rifamycin, and ethambutol that is continued for 12 months beyond sputum culture conversion to negative. MAC-PD is difficult to treat, with frequent drug-related side effects and suboptimal treatment outcomes. Refractory and recurrent disease is common, leading to lifelong follow-up of patients. There are limited treatment options for patients with macrolide-resistant or refractory disease. Amikacin liposome inhalation suspension is recommended for treatment-refractory patients whose cultures remain positive after 6 months of guideline-based therapy. Among the research priorities to improve patient outcomes and quality of life are developing new, more rapid diagnostic tests, investigating biomarkers associated with disease progression, and identifying new drugs and routes of administration as well as new, shorter, and better-tolerated regimens.
Carprofen elicits pleiotropic mechanisms of bactericidal action with the potential to reverse antimicrobial drug resistance in tuberculosis
Authors: Maitra Arundhati , Evangelopoulos Dimitrios , Chrzastek Alina , Martin Liam T , Hanrath Aidan , Chapman Ellie , Hailes Helen C , Lipman Marc , McHugh Timothy D , Waddell Simon J , Bhakta Sanjib 
Published: June 2020

AbstractBackgroundThe rise of antimicrobial drug resistance in Mycobacterium tuberculosis coupled with the shortage of new antibiotics has elevated TB to a major global health priority. Repurposing drugs developed or used for other conditions has gained special attention in the current scenario of accelerated drug development for several global infectious diseases. In a similar effort, previous studies revealed that carprofen, a non-steroidal anti-inflammatory drug, selectively inhibited the growth of replicating, non-replicating and MDR clinical isolates of M. tuberculosis.ObjectivesWe aimed to reveal the whole-cell phenotypic and transcriptomic effects of carprofen in mycobacteria.MethodsIntegrative molecular and microbiological approaches such as resazurin microtitre plate assay, high-throughput spot-culture growth inhibition assay, whole-cell efflux inhibition, biofilm inhibition and microarray analyses were performed. Analogues of carprofen were also synthesized and assessed for their antimycobacterial activity.ResultsCarprofen was found to be a bactericidal drug that inhibited mycobacterial drug efflux mechanisms. It also restricted mycobacterial biofilm growth. Transcriptome profiling revealed that carprofen likely acts by targeting respiration through the disruption of membrane potential. The pleiotropic nature of carprofen’s anti-TB action may explain why spontaneous drug-resistant mutants could not be isolated in practice.ConclusionsThis immunomodulatory drug and its chemical analogues have the potential to reverse TB antimicrobial drug resistance, offering a swift path to clinical trials of novel TB drug combinations.
Clofazimine pharmacokinetics in patients with TB: dosing implications
Authors: Abdelwahab Mahmoud Tareq , Wasserman Sean , Brust James C M , Gandhi Neel R , Meintjes Graeme , Everitt Daniel , Diacon Andreas , Dawson Rodney , Wiesner Lubbe , Svensson Elin M , Maartens Gary , Denti Paolo 
Published: June 2020

Abstract

Background
Clofazimine is in widespread use as a key component of drug-resistant TB regimens, but the recommended dose is not evidence based. Pharmacokinetic data from relevant patient populations are needed to inform dose optimization.

Objectives
To determine clofazimine exposure, evaluate covariate effects on variability, and simulate exposures for different dosing strategies in South African TB patients.

Patients and methods
Clinical and pharmacokinetic data were obtained from participants with pulmonary TB enrolled in two studies with intensive and sparse sampling for up to 6 months. Plasma concentrations were measured by LC-MS/MS and interpreted with non-linear mixed-effects modelling. Body size descriptors and other potential covariates were tested on pharmacokinetic parameters. We simulated different dosing regimens to safely shorten time to average daily concentration above a putative target concentration of 0.25 mg/L.

Results
We analysed 1570 clofazimine concentrations from 139 participants; 79 (57%) had drug-resistant TB and 54 (39%) were HIV infected. Clofazimine pharmacokinetics were well characterized by a three-compartment model. Clearance was 11.5 L/h and peripheral volume 10 500 L for a typical participant. Lower plasma exposures were observed in women during the first few months of treatment, explained by higher body fat fraction. Model-based simulations estimated that a loading dose of 200 mg daily for 2 weeks would achieve average daily concentrations above a target efficacy concentration 37 days earlier in a typical TB participant.

Conclusions
Clofazimine was widely distributed with a long elimination half-life. Disposition was strongly influenced by body fat content, with potential dosing implications for women with TB.
Inherent privacy limitations of decentralized contact tracing apps
Authors: Bengio Yoshua , Ippolito Daphne , Janda Richard , Jarvie Max , Prud'homme Benjamin , Rousseau Jean-François , Sharma Abhinav , Yu Yun William 
Published: June 2020

Abstract
Recently, there have been many efforts to use mobile apps as an aid in contact tracing to control the spread of the SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) (COVID-19 [coronavirus disease 2019]) pandemic. However, although many apps aim to protect individual privacy, the very nature of contact tracing must reveal some otherwise protected personal information. Digital contact tracing has endemic privacy risks that cannot be removed by technological means, and which may require legal or economic solutions. In this brief communication, we discuss a few of these inherent privacy limitations of any decentralized automatic contact tracing system.
Analytical Quality by Design as an Important Tool to Determine the Best Analytical Conditions for Isoniazid and Its Respective Succinylated Prodrug
Authors: Rampini Denise , de Araújo Renan Vinícius , Foitinho Rodrigo Esteves , Lourenço Felipe Rebello , Giarolla Jeanine 
Published: June 2020

Abstract

Background
Tuberculosis is a worldwide health concern and isoniazid is the most used and considered one of the most effective drugs for its treatment. The “quality” concept must be incorporated into the final pharmaceutical product, according to the quality by design (QbD) definition. Therefore, the determination of analytical test conditions is extremely important and the design of experiments (DoE) becomes a very useful tool.

Objective
This paper used the concept of QbD to assist the development of analytical conditions for isoniazid and its respective prodrug, applying HPLC.

Method
HPLC analytical methodologies were developed for isoniazid and its succinylated derivative. The experimental design was carried out using three analytical parameters at three levels. Four chromatographic responses were studied. The impact of analytical parameters on chromatographic responses was assessed using a Pareto chart. Regression models were obtained using multiple regression analysis. DoE analysis was conducted using the Minitab® program and the experiments were performed sequentially, with varying factors.

Results
We identify three main risk parameters: mobile phase (high), flow rate (moderate), and pH of buffer (moderate). The ratio of mobile phase buffer (X2) and mobile phase pH (X3) had a major influence on the peak resolutions (Y3). The capacity factors for iso-suc (Y1) and isoniazid (Y2) peaks should be within 3–9 and 4–10, respectively. The peak resolutions between iso-suc and isoniazid (Y3) should be above two.

Conclusions
We designed 27 experiments, obtaining 1.0 mL/min flow rate, 95% buffer in the mobile phase, and pH 7.0 as the optimal analytical conditions.

Highlights
Analytical Quality by Design was used as an important tool to determine the best analytical test conditions for isoniazid and its respective prodrug - succinylated isoniazid
Development of a Loop-Mediated Isothermal Amplification Assay as an Early-Warning Tool for Detecting Emerald Ash Borer (Coleoptera: Buprestidae) Incursions
Authors: Kyei-Poku George , Gauthier Debbie , Quan Guoxing 
Published: June 2020

AbstractThe emerald ash borer (EAB), Agrilus planipennis (Fairmaire), is the most destructive invasive insect species of ash (Fraxinus spp.) in North America. An accurate method for early detection of this noxious insect pest is indispensable to providing adequate warning of A. planipennis infestation. A loop-mediated isothermal amplification (LAMP) assay (EAB-LAMP) was developed based on mitochondrial cytochrome c oxidase subunit I (COI) gene. The EAB-LAMP required only 30 min at 65°C to amplify A. planipennis DNA from specimens collected from geographically distinct locations. There was no cross-reactivity with other Agrilus and insect species. The developed EAB-LAMP differentially detected traces of A. planipennis genome (COI) within frass from various Fraxinus species. EAB-LAMP was also able to distinguish among A. planipennis DNA and other Agrilus species and nontarget insect species in trap captures. By detecting A. planipennis DNA in two additional trap captures (in situ), the EAB-LAMP was more sensitive and reliable than visual inspection. We tested the quantitative nature of the assay by evaluating pooled trap samples and demonstrated that the EAB-LAMP was capable of functioning optimally using a pool size of at least five individual trap samples. This potentially circumvents the need to perform large-scale individual analysis for processing trap samples. Considering its performance, specificity, sensitivity, and repeatability, the developed EAB-LAMP could be a valuable tool to support strategy and operation of large-scale surveillance for A. planipennis and could profitably be used in routine monitoring programs for effective management of A. planipennis.
Field Edge and Field-to-Field Ecotone-Type Influences on Two Cotton Herbivores: Cotton Fleahopper, Pseudatomoscelis seriatus (Hemiptera: Miridae), and Verde Plant Bug, Creontiades signatus
Authors: Esquivel Isaac L , Brewer Michael J , Coulson Robert N 
Published: June 2020

Abstract
In the United States, the average field size has roughly doubled from the 1980s to the mid-2000s, while average cropland has stayed the same. This will likely influence how semi-natural habitats and edges affect local patterns and processes such as natural pest control or pest densities. We hypothesized that densities of two cotton pests, cotton fleahopper (Pseudatomoscelis seriatus) and verde plant bug (Creontiades signatus) (Hemiptera: Miridae), and corresponding cotton injury in a cotton agroecosystem were affected by field edge, ecotone type (described by the neighboring habitat), and the influence of ecotone type on edge effects. Studies over 2 yr using transect and random point sampling indicated that densities of both insects declined significantly and in a linear fashion from the cotton field boundary (0 m) to field interior (200–300 m from field edge). The decline was influenced by ecotone type for cotton fleahopper. Pest densities in cotton at the interface with semi-natural habitat were higher but declined at a greater rate into the cotton field interior compared to densities seen at the interfaces with sorghum or another cotton field. These effects were also observed for verde plant bug and the cotton boll injury it causes. Regardless of the pest densities near the field edge and the rate of decline into the field interior, densities beyond 100 m into the field were up to 70% less than field edges for both insect species and for boll injury. Potential for land managers to improve sampling efficiency when scouting is apparent. For example, pest species may be at economic threshold in certain parts of the field but not others, leading to different management decisions in larger fields. Therefore, for cotton fleahopper and verde plant bug, edges should be the focus of initial pest detection and sampling, and interior field sampling may only be required when edges are above the economic threshold.