bluuebunny/crossref_metadata_2025_split huggingface dataset:
Serum selenium level and cancer risk: a nested case-control study
Authors: Narod Steven A. , Huzarski Tomasz , Jakubowska Anna , Gronwald Jacek , Cybulski Cezary , Oszurek Oleg , Dębniak Tadeusz , Jaworska-Bieniek Katarzyna , Lener Marcin , Białkowska Katarzyna , Sukiennicki Grzegorz , Muszyńska Magdalena , Marciniak Wojciech , Sun Ping , Kotsopoulos Joanne , Lubiński Jan 
Published: December 2019

Abstract
Background
Epidemiologic studies have demonstrated a relationship between selenium status and cancer risk among those with low selenium levels. It is of interest to prospectively evaluate the relationship between selenium and cancer among women who reside in a region with ubiquitously low selenium levels.

Methods
We performed a nested case-control study of baseline serum selenium levels and cancer risk using data and biological samples from 19,573 females that were participants in a biobanking initiative between 2010 and 2014 in Szczecin Poland. Cases included women with any incident cancer (n = 97) and controls (n = 184) were women with no cancer at baseline or follow-up. Serum selenium was quantified using mass spectroscopy.

Results
The odds ratio associated being below the cutoff of 70.0 μg/L compared to a level above 70.0 μg/L was 2.29 (95% CI 1.26–4.19; P = 0.007). The risks for women in the two middle categories were similar and suggests that the normal range be between 70 μg/L and 90 μg/L. There was evidence for an increased risk of cancer among women in the highest category of selenium levels (i.e., > 90 μg/L), but this association did not achieve statistical significance (OR = 1.63; 95%CI 0.63–4.19; P = 0.31).

Conclusions
Results from this study suggest that suggest that the optimum serum level of selenium in women living in Poland should be between 70 μg/L and 90 μg/L.
RADAR: differential analysis of MeRIP-seq data with a random effect model
Authors: Zhang Zijie , Zhan Qi , Eckert Mark , Zhu Allen , Chryplewicz Agnieszka , De Jesus Dario F. , Ren Decheng , Kulkarni Rohit N. , Lengyel Ernst , He Chuan , Chen Mengjie 
Published: December 2019

AbstractEpitranscriptome profiling using MeRIP-seq is a powerful technique for in vivo functional studies of reversible RNA modifications. We develop RADAR, a comprehensive analytical tool for detecting differentially methylated loci in MeRIP-seq data. RADAR enables accurate identification of altered methylation sites by accommodating variability of pre-immunoprecipitation expression level and post-immunoprecipitation count using different strategies. In addition, it is compatible with complex study design when covariates need to be incorporated in the analysis. Through simulation and real dataset analyses, we show that RADAR leads to more accurate and reproducible differential methylation analysis results than alternatives, which is available at https://github.com/scottzijiezhang/RADAR.
The ethical challenges raised in the design and conduct of pragmatic trials: an interview study with key stakeholders
Authors:   on behalf of the Ethics of Pragmatic Trials project, Nicholls Stuart G. , Carroll Kelly , Zwarenstein Merrick , Brehaut Jamie C. , Weijer Charles , Hey Spencer P. , Goldstein Cory E. , Graham Ian D. , Grimshaw Jeremy M. , McKenzie Joanne E. , Fergusson Dean A. , Taljaard Monica 
Published: December 2019

AbstractBackgroundThere is a concern that the apparent effectiveness of interventions tested in clinical trials may not be an accurate reflection of their actual effectiveness in usual practice. Pragmatic randomized controlled trials (RCTs) are designed with the intent of addressing this discrepancy. While pragmatic RCTs may increase the relevance of research findings to practice they may also raise new ethical concerns (even while reducing others). To explore this question, we interviewed key stakeholders with the aim of identifying potential ethical challenges in the design and conduct of pragmatic RCTs with a view to developing future guidance on these issues.MethodsInterviews were conducted with clinical investigators, methodologists, patient partners, ethicists, and other knowledge users (e.g., regulators). Interviews covered experiences with pragmatic RCTs, ethical issues relevant to pragmatic RCTs, and perspectives on the appropriate oversight of pragmatic RCTs. Interviews were coded inductively by two coders. Interim and final analyses were presented to the broader team for comment and discussion before the analytic framework was finalized.ResultsWe conducted 45 interviews between April and September 2018. Interviewees represented a range of disciplines and jurisdictions as well as varying content expertise. Issues of importance in pragmatic RCTs were (1) identification of relevant risks from trial participation and determination of what constitutes minimal risk; (2) determining when alterations to traditional informed consent approaches are appropriate; (3) the distinction between research, quality improvement, and practice; (4) the potential for broader populations to be affected by the trial and what protections they might be owed; (5) the broader range of trial stakeholders in pragmatic RCTs, and determining their roles and responsibilities; and (6) determining what constitutes “usual care” and implications for trial reporting.ConclusionsOur findings suggest both the need to discuss familiar ethical topics in new ways and that there are new ethical issues in pragmatic RCTs that need greater attention. Addressing the highlighted issues and developing guidance will require multidisciplinary input, including patient and community members, within a broader and more comprehensive analysis that extends beyond consent and attends to the identified considerations relating to risk and stakeholder roles and responsibilities.
Recent advances in the use of benzimidazoles as corrosion inhibitors
Authors: Marinescu Maria 
Published: December 2019

AbstractBackgroundBenzimidazole, a key heterocycle in therapeutic chemistry, and its derivatives, are recently mentioned in the literature as corrosion inhibitors for steels (CS, MS), pure metals (Fe, Al, Cu, Zn) and alloys. Benzimidazoles are good corrosion inhibitors for extremely aggressive, corrosive acidic media such as 1 M HCl, 1 M HNO3, 1.5 M H2SO4, basic media, 0.1 M NaOH or salt solutions. Benzimidazole derivatives act as mixed type inhibitors, exhibiting stronger inhibitive effect on the cathodic reaction than on the anodic one.ConclusionThese review highlights recent research in the field of benzimidazole compounds that their role as corrosion inhibitors, the structure of the compounds, electrochemical studies, the experimental conditions, the proposed mechanisms as well as the quantum theoretical studies that predict the structure of the compounds with inhibition properties.
Engineering the oleaginous yeast Yarrowia lipolytica for production of α-farnesene
Authors: Liu Yinghang , Jiang Xin , Cui Zhiyong , Wang Zhaoxuan , Qi Qingsheng , Hou Jin 
Published: December 2019

AbstractBackgroundYarrowia lipolytica, a non-traditional oil yeast, has been widely used as a platform for lipid production. However, the production of other chemicals such as terpenoids in engineeredY. lipolyticais still low. α-Farnesene, a sesquiterpene, can be used in medicine, bioenergy and other fields, and has very high economic value. Here, we used α-farnesene as an example to explore the potential ofY. lipolyticafor terpenoid production.ResultsWe constructed libraries of strains overexpressing mevalonate pathway and α-farnesene synthase genes by non-homologous end-joining (NHEJ) mediated integration into theY. lipolyticachromosome. First, a mevalonate overproduction strain was selected by overexpressing relevant genes and changing the cofactor specificity. Based on this strain, the downstream α-farnesene synthesis pathway was overexpressed by iterative integration. Culture conditions were also optimized. A strain that produced 25.55 g/L α-farnesene was obtained. This is the highest terpenoid titer reported inY. lipolytica.ConclusionsYarrowia lipolyticais a potentially valuable species for terpenoid production, and NHEJ-mediated modular integration is effective for expression library construction and screening of high-producer strains.
Inhibition of Nrf2/HO-1 signaling leads to increased activation of the NLRP3 inflammasome in osteoarthritis
Authors: Chen Zhuming , Zhong Huan , Wei Jinsong , Lin Sien , Zong Zhixian , Gong Fan , Huang Xinqia , Sun Jinhui , Li Peng , Lin Hao , Wei Bo , Chu Jiaqi 
Published: December 2019

AbstractIntroductionOsteoarthritis (OA) is an inflammatory disease of the joints that causes progressive disability in the elderly. Reactive oxygen species (ROS) play an important role in OA development; they may activate the NLRP3 inflammasome, thereby inducing the secretion of proinflammatory IL-1β and IL-18, leading to the aggravation of the downstream inflammatory response. Nrf2 is a key transcription factor that regulates the expression of antioxidant enzymes that protect against oxidative stress and tissue damage. We aimed to explore the underlying mechanism of OA development by investigating NLRP3, ASC, Nrf2, and HO-1 expression in synovia and their regulatory networks in OA.MethodsHuman total knee replacement samples were subjected to histology and micro-CT analysis to determine the pathological changes in the cartilage and subchondral bone and to assess the expression of inflammation-related markers in the synovial tissue by immunohistochemistry (IHC), qRT-PCR, and Western blot. To investigate these pathological changes in an OA animal model, adult Sprague-Dawley rats were subjected to anterior cruciate ligament transection and medial meniscectomy. Articular cartilage and subchondral bone changes and synovial tissue were also determined by the same methods used for the human samples. Finally, SW982 cells were stimulated with lipopolysaccharide (LPS) as an in vitro inflammatory cell model. The correlation between NLRP3 and Nrf2 expression was confirmed by knocking down NLRP3 or Nrf2.ResultsCartilage destruction and subchondral bone sclerosis were found in the OA patients and OA model rats. Significantly increased expression levels of NLRP3, ASC, Nrf2, and HO-1 were found in the synovial tissue from OA patients. NLRP3, ASC, Nrf2, and HO-1 expression in the synovium was also upregulated in the OA group compared with the sham group. Furthermore, the NLRP3, Nrf2, HO-1, IL-1β, and IL-18 expression in LPS-treated SW982 cells was increased in a dose-dependent manner. As expected, the expression of NLRP3 was upregulated, and the expression of IL-1β and IL-18 was downregulated after Nrf2 silencing. However, knocking down NLRP3 did not affect the expression of Nrf2.ConclusionsROS-induced oxidative stress may be the main cause of NLRP3 inflammasome activation and subsequent release of downstream factors during OA development. Nrf2/HO-1 signaling could be a key pathway for the activation of the NLRP3 inflammasome, which may contribute to the progression of OA. Herein, we discovered a novel role of Nrf2/HO-1 signaling in the production of NLRP3, which may facilitate the prevention and treatment of OA.
Association of the mean platelet volume and red cell distribution width with dipper and non-dipper blood pressure in prehypertensive non-smokers
Authors: Taban Sadeghi Mohammadreza , Soroureddin Zahra , Nouri-Vaskeh Masoud , Nazarpoori Pantea , Aghayari Sheikh Neshin Saeideh 
Published: December 2019

Abstract
Objective
Absence of nocturnal blood pressure (BP) dipping is associated with poor health outcomes, including increased mortality. Non-dipper BP seems to be a predictor of cardiovascular damage in hypertensive patients. The aim of this study was to investigate the association of the mean platelet volume (MPV) and red cell distribution width (RDW) with nocturnal dipping/non dipping status in newly diagnosed and untreated prehypertensive non-smokers, using ambulatory BP monitoring.

Results
Twenty-eight patients (15 males) in the dipper group and 24 patients (11 males) in the non-dipper group were evaluated in this study. The age of patients was 41.64 ± 15.01 and 37.96 ± 15.08 years in the dipper and non-dipper groups, respectively. The rate of nocturnal systolic BP drop in the dipper and non-dipper groups was 13.79 ± 3.35% (10.20–22.10) and 5.96 ± 2.87% (1.10–9.30) (P < 0.001), respectively. Also, the mean rate of nocturnal diastolic BP drop in the dipper and non-dipper groups was 17.02 ± 5.09% (10.30–26.90) and 6.19 ± 2.75% (1.20–9.70) (P < 0.001), respectively. RDW and MPV were significantly higher in non-dipper patients than dipper patients (P = 0.001 and P = 0.012, respectively). Bivariate analysis revealed that MPV was inversely correlated with the nocturnal systolic BP drop (P = 0.005, r = − 0.385). Furthermore, RDW was inversely correlated with systolic BP drop (P = 0.019, r = − 0.324).
Dose-dependent roles of aspirin and other non-steroidal anti-inflammatory drugs in abnormal bone remodeling and skeletal regeneration
Authors: Xie Yong , Pan Meng , Gao Yanpan , Zhang Licheng , Ge Wei , Tang Peifu 
Published: December 2019

AbstractThe failure of remodeling process that constantly regenerates effete, aged bone is highly associated with bone nonunion and degenerative bone diseases. Numerous studies have demonstrated that aspirin and other non-steroidal anti-inflammatory drugs (NSAIDs) activate cytokines and mediators on osteoclasts, osteoblasts and their constituent progenitor cells located around the remodeling area. These cells contribute to a complex metabolic scenario, resulting in degradative or synthetic functions for bone mineral tissues. The spatiotemporal effects of aspirin and NSAIDs in the bone remodeling are controversial according the specific therapeutic doses used for different clinical conditions. Herein, we review in vitro, in vivo, and clinical studies on the dose-dependent roles of aspirin and NSAIDs in bone remodeling. Our results show that low-dose aspirin (< 100 μg/mL), which is widely recommended for prevention of thrombosis, is very likely to be benefit for maintaining bone mass and qualities by activation of osteoblastic bone formation and inhibition of osteoclast activities via cyclooxygenase-independent manner. While, the roles of high-dose aspirin (150–300 μg/mL) and other NSAIDs in bone self-regeneration and fracture-healing process are difficult to elucidate owing to their dual effects on osteoclast activity and bone formation of osteoblast. In conclusion, this study highlighted the potential clinical applications of low-dose aspirin in abnormal bone remodeling as well as the risks of high-dose aspirin and other NSAIDs for relieving pain and anti-inflammation in fractures and orthopedic operations.
Efficient cover image selection based on spatial block analysis and DCT embedding
Authors: Abed Sa’ed , Al-Roomi Suood Abdulaziz , Al-Shayeji Mohammad 
Published: December 2019

AbstractIn steganography, the cover medium is widely treated as a mere container for the embedded information, even though it affects the stego-image quality, security, and robustness. In addition, there is no consensus on the characteristics of a suitable cover image. In this work, we introduce and practically prove the most suitable cover image (MSCI) framework to automatically select a cover image for a given secret image. This paper proposes choosing the most suitable cover from a set of images based on two steps. First, a set of cover images is filtered based on relative entropy and a histogram in order to identify the most suitable candidates. Second, the local block pixel intensity features of the candidates are analyzed to select the most suitable cover image. Furthermore, cover image local blocks were optimized, using rotation and flipping, during the embedding process to further improve stego-image representation. The proposed framework demonstrated high visual image quality when compared with existing solutions. Steganalysis tests indicated that the proposed solution for cover selection provided an increased resistance to modern steganalyzers with up to 30% lowered detection rate, which improved security.
Insect pollinator dependence of shea (Vitellaria paradoxa C.F. Gaertn.) in the Guinea Savanna zone of Ghana
Authors: Nasare Latif Iddrisu , Kwapong Peter K. , Doke Dzigbodi Adzo 
Published: December 2019

AbstractShea (Vitellaria paradoxa C.F. Gaertn.) is a multipurpose tree species indigenous to the Sudano Sahelian zone of Africa and occurs as the most abundant economic tree species in northern Ghana. The edible oil (shea butter) extracted from shea kernel is ranked as the most economic product of the species. Although fruit set and yield of shea are known to be influenced by insect pollination, the actual contribution of insect pollinators to its reproductive success has rarely been studied. This study estimated the percentage insect pollinator dependence and monetary value of insect pollination per bag of shea kernel (85 kg) in the Guinea savanna zone. Open pollination and insect exclusion treatments were applied to the flowers of 18 randomly selected matured shea trees and observed from the onset of flowering to fruit maturity. Proportion of total production value attributed to insect pollination approach was used in estimating the monetary value of pollination per bag of shea kernel with the average market price as proxy. The study revealed an insect pollinator dependence of 77% and 73% for fruit set and dry kernel yield, respectively. Mean number of fruit set per inflorescence varied significantly between insect-excluded and open-pollinated inflorescences (p < 0.05). The monetary value of insect pollination was estimated at GH₵ 73.21 (US$ 18.67) per bag of kernel as of August 2016. Shea is a high insect pollinator–dependent species and the conservation of insect pollinators would be critical to the sustainability of yield.
School functioning and internalizing problems in young schoolchildren
Authors: Pedersen Marit Løtveit , Holen Solveig , Lydersen Stian , Martinsen Kristin , Neumer Simon-Peter , Adolfsen Frode , Sund Anne Mari 
Published: December 2019

AbstractBackgroundSymptoms of anxiety and depression are common mental health problems in children and are often referred to as internalizing symptoms. Youth with such symptoms are at greater risk for poor academic achievement, school non-completion, and future mental health problems, all of which, lead to public health consequences and costs to society. The aim of the current study was to investigate associations between young school children’s internalizing symptoms and school functioning, as assessed separately by the teachers and the children.MethodsThis study is a cross-sectional study including children (N = 750. 58% girls) from the ages of 8–12 years with elevated levels of self-reported symptoms of anxiety (MASC-C) and/or depression (SMFQ). Teachers reported the academic achievement, school adaptation (TRF) and internalizing symptoms (BPM-T) of the children.Associations were analyzed using linear regression analyses.ResultsBoth teacher-reported internalizing symptoms and children’s self-reported depressive symptoms were associated with poor academic achievement and school adaptation, while self-reported symptoms of anxiety were not. Symptoms of depression as assessed by the children were associated with teacher-rated internalizing symptoms, while self-reported symptoms of anxiety were not.ConclusionWe found negative associations between school functioning and internalizing symptoms, as assessed by both the teachers and the children. The dual findings strengthen the validity of these relationships. Thus, prevention of depressive and anxiety symptoms in children may lead to positive changes in school domains such as academic achievement and school adaptation. The weak and non-significant associations between teacher-rated internalizing problems and children`s self-report on depression- and anxiety symptoms respectively, indicate that teachers may have difficulties recognizing children with these symptoms.Trial registrationClinical TrialsNCT02340637, Registered on June 12, 2014, Retrospectively registered.
Sleep deprivation of rats increases postsurgical expression and activity of L-type calcium channel in the dorsal root ganglion and slows recovery from postsurgical pain
Authors: Li Qi , Zhu Zi-yu , Lu Jian , Chao Yu-Chieh , Zhou Xiao-xin , Huang Ying , Chen Xue-mei , Su Dian-san , Yu Wei-feng , Gu Xi-yao 
Published: December 2019

AbstractPerioperative sleep disturbance is a risk factor for persistent pain after surgery. Clinical studies have shown that patients with insufficient sleep before and after surgery experience more intense and long-lasting postoperative pain. We hypothesize that sleep deprivation alters L-type calcium channels in the dorsal root ganglia (DRG), thus delaying the recovery from post-surgical pain. To verify this hypothesis, and to identify new predictors and therapeutic targets for persistent postoperative pain, we first established a model of postsurgical pain with perioperative sleep deprivation (SD) by administering hind paw plantar incision to sleep deprivation rats. Then we conducted behavioral tests, including tests with von Frey filaments and a laser heat test, to verify sensory pain, measured the expression of L-type calcium channels using western blotting and immunofluorescence of dorsal root ganglia (an important neural target for peripheral nociception), and examined the activity of L-type calcium channels and neuron excitability using electrophysiological measurements. We validated the findings by performing intraperitoneal injections of calcium channel blockers and microinjections of dorsal root ganglion cells with adeno-associated virus. We found that short-term sleep deprivation before and after surgery increased expression and activity of L-type calcium channels in the lumbar dorsal root ganglia, and delayed recovery from postsurgical pain. Blocking these channels reduced impact of sleep deprivation. We conclude that the increased expression and activity of L-type calcium channels is associated with the sleep deprivation-mediated prolongation of postoperative pain. L-type calcium channels are thus a potential target for management of postoperative pain.