bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparison of the pathogenicity of Francisella orientalis in Nile tilapia (Oreochromis niloticus), Asian seabass (Lates calcarifer) and largemouth bass (Micropterus salmoides) through experimental intraperitoneal infection
Authors: Poudyal Sayuj , Pulpipat Theeraporn , Wang Pei‐Chi , Chen Shih‐Chu 
Published: July 2020

AbstractFrancisella orientalis is a highly virulent, emerging bacterium that causes mass mortalities in tilapia. This pathogen also affects numerous other warm‐water fish species, including three‐line grunt, hybrid striped bass and various ornamental fish. This study sheds light on two new species of fish that are susceptible to F. orientalis. Asian seabass and largemouth bass showed variable levels of susceptibility in a bacterial challenge experiment. After intraperitoneally injected with a dose of 106 CFU/fish, a total of 64.28% and 21.42% mortalities were obtained in Asian seabass and largemouth bass, respectively. Meanwhile, Nile tilapia showed acute mortality of 100%. All fish showed typical lesions of francisellosis, including multifocal granulomas in the spleen and head kidney. Immunohistochemical analysis revealed strong positive signals inside the granulomas of all fish. The bacterial recovery in solid media from infected fish was highest in Nile tilapia (85.71%), followed by Asian seabass (35.71%) and largemouth bass (21.42%). PCR results tested 100% positive for Nile tilapia, and 78.57% and 21.42% for Asian seabass and largemouth bass, respectively. In conclusion, Asian seabass and largemouth bass are susceptible to this pathogen, which warrants new management strategies when employing predation polyculture systems of these species with tilapia.
National Trends in Potentially Preventable Hospitalizations of Older Adults with Dementia
Authors: Anderson Timothy S. , Marcantonio Edward R. , McCarthy Ellen P. , Herzig Shoshana J. 
Published: July 2020

BACKGROUND/OBJECTIVESDementia is associated with higher healthcare expenditures, in large part due to increased hospitalization rates relative to patients without dementia. Data on contemporary trends in the incidence and outcomes of potentially preventable hospitalizations of patients with dementia are lacking.DESIGNRetrospective cohort study using the National Inpatient Sample from 2012 to 2016.SETTINGU.S. acute care hospitals.PARTICIPANTSA total of 1,843,632 unique hospitalizations of older adults (aged ≥65 years) with diagnosed dementia.MEASUREMENTSAnnual trends in the incidence of hospitalizations for all causes and for potentially preventable conditions including acute ambulatory care sensitive conditions (ACSCs), chronic ACSCs, and injuries. In‐hospital outcomes including mortality, discharge disposition, and hospital costs.RESULTSThe survey weighted sample represented an estimated 9.27 million hospitalizations for patients with diagnosed dementia (mean [standard deviation] age = 82.6 [6.7] years; 61.4% female). In total, 3.72 million hospitalizations were for potentially preventable conditions (40.1%), 2.07 million for acute ACSCs, .76 million for chronic ACSCs, and .89 million for injuries. Between 2012 and 2016, the incidence of all‐cause hospitalizations declined from 1.87 million to 1.85 million per year (P = .04) while the incidence of potentially preventable hospitalizations increased from .75 million to .87 million per year (P < .001), driven by an increased number of hospitalizations of community‐dwelling older adults. Among patients with dementia hospitalized for potentially preventable conditions, inpatient mortality declined from 6.4% to 6.1% (P < .001), inflation‐adjusted median costs increased from $7,319 to $7,543 (P < .001), and total annual costs increased from $7.4 to $9.3 billion. Although 86.0% of hospitalized patients were admitted from the community, only 32.7% were discharged to the community.CONCLUSIONThe number of potentially preventable hospitalizations of older adults with dementia is increasing, driven by hospitalizations of community‐dwelling older adults. Improved strategies for early detection and goal‐directed treatment of potentially preventable conditions in patients with dementia are urgently needed. J Am Geriatr Soc 68:2240–2248, 2020.
Subcellular structure and behaviour in fungal hyphae
Authors: ROBERSON R.W. 
Published: July 2020

SummaryThis work briefly surveys the diversity of selected subcellular characteristics in hyphal tip cells of the fungal kingdom (Mycota). Hyphae are filamentous cells that grow by tip extension. It is a highly polarised mechanism that requires a robust secretory system for the delivery of materials (e.g. membrane, proteins, cell wall materials) to sites of cell growth. These events result it the self‐assembly of a Spitzenkörper (Spk), found most often in the Basidiomycota, Ascomycota, and Blastocladiomycota, or an apical vesicle crescent (AVC), present in the most Mucoromycota and Zoopagomycota. The Spk is a complex apical body composed of secretory vesicles, cytoskeletal elements, and signaling proteins. The AVC appears less complex, though little is known of its composition other than secretory vesicles. Both bodies influence hyphal growth and morphogenesis. Other factors such as cytoskeletal functions, endocytosis, cytoplasmic flow, and turgor pressure are also important in sustaining hyphal growth. Clarifying subcellular structures, functions, and behaviours through bioimagining analysis are providing a better understanding of the cell biology and phylogenetic relationships of fungi.Lay DescriptionFungi are most familiar to the public as yeast, molds, and mushrooms. They are eukaryotic organisms that inhabit diverse ecological niches around the world and are critical to the health of ecosystems performing roles in decomposition of organic matter and nutrient recycling (Heath, 1990). Fungi are heterotrophs, unlike plants, and comprise the most successful and diverse phyla of eukaryotic microbes, interacting with all other forms of life in associations that range from beneficial (e.g., mycorrhizae) to antagonistic (e.g., pathogens). Some fungi can be parasitic or pathogenic on plants (e.g., Cryphonectria parasitica, Magnaporthe grisea), insects (e.g., Beauveria bassiana, Cordyceps sp.), invertebrates (e.g., Drechslerella anchonia), vertebrates (e.g., Coccidioides immitis, Candia albicans) and other fungi (e.g., Trichoderma viride, Ampelomyces quisqualis). The majority of fungi, however, are saprophytes, obtaining nutrition through the brake down of non‐living organic matter.
User involvement in the implementation of welfare technology in home care services: The experience of health professionals—A qualitative study
Authors: Glomsås Heidi Snoen , Knutsen Ingrid Ruud , Fossum Mariann , Halvorsen Kristin 
Published: July 2020

AbstractAims and objectivesThe aim of this study is to learn more about factors that promote or inhibit user involvement among health professionals when implementing welfare technology in home care services.BackgroundIt is a health policy goal to increase the use of welfare technology in order to address some of the challenges that healthcare services are facing. Health professionals' involvement is important for the successful implementation of welfare technology in home care services.DesignThe study has an explorative and descriptive longitudinal design based on a qualitative approach. Five focus group interviews were conducted with 16 nurses and assistant nurses from three different municipalities over a period of 2 years. The data were analysed using reflexive thematic analysis. The COREQ checklist was used.ResultsThe analysis led to five main themes: competence a critical component, information and information lines, new ways of working, choice of welfare technology and change in patient services. From health professionals' perspective, there appeared to be a lack of preparedness for the change in the implementation of welfare technology entailed for home care services. The respondents experienced limited facilitation and opportunities for user involvement.ConclusionHealth professionals want to be more involved but emphasised that competence, information and collaborative arenas are necessary factors if involvement in the process is to be increased. Competence affected some of the respondents' attitudes and willingness to use the technology. The respondents also experienced that the managements' focus on facilitation and interest in user involvement in addition to infrastructures that functioned in various ways had an impact on user involvement.Relevance to clinical practiceThe knowledge gained from this study about factors that promote or inhibit user involvement among health professionals could contribute to better preparedness for further implementation of welfare technology in the field of home care.
Prognostic factors and surgical treatment in vulvar carcinoma: Single center experience
Authors: Pecorino Basilio , Scibilia Giuseppe , Ferrara Martina , Di Stefano Andrea Benedetto , D'Agate Maria Gabriella , Giambanco Laura , Scollo Paolo 
Published: July 2020

AbstractAimVulvar carcinoma represents 3–5% of all female genital cancers; the main surgical treatment is radical vulvectomy and inguinal lymphadenectomy. The aim of this study is to analyze prognostic factors in the patients underwent to primary surgery for vulvar carcinoma.MethodsOne hundred and eighteen cases of vulvar carcinoma underwent surgery between 2006 and 2016 at Operative Unit of Gynecology and Obstetrics of Cannizzaro Hospital (Catania, Italy) were retrospective analyzed. Risk factors for relapse (age, tumor size, FIGO stage, type of surgery, lymphadenectomy, margins status, metastatic nodes and radiotherapy) were evaluated by logistic regression. Univariate analysis of prognostic factors (age, tumor size, FIGO stage, metastatic inguinal nodes and type of surgery) was obtained by Cox proportional hazard model. Overall survival was calculated by Kaplan–Meier curves either for the entire population and for comparison between positive and negative variables (margin status, nodes and radiotherapy) with log‐rank test to determine significance. Statistical significance was reached for P < 0.05.ResultsType of surgery (radical local excision vs. radical vulvectomy) and positive inguinal nodes were identified as risk factors for relapse. Positive inguinal nodes and positive margins were identified as prognostic factors either for overall survival and disease specific survival; tumor size greater than 4 cm was identified as prognostic factors for overall survival. Overall survival was 38.4% and it was significantly higher in the patients with negative margins and nodes.ConclusionsNodes status, resection margins, age and type of surgery represent prognostic factors have to be considered for adjuvant treatment in the patients affected from vulvar carcinoma.
Labor Market Outcomes of Professional Women with Two Children after the One‐Child Policy in China
Authors: Shen Yang , Jiang Lai 
Published: July 2020

AbstractSince the implementation of the two‐child policy in China in 2016, it is unclear how professional women's labor force outcomes and family commitments have changed. Using interviews with 26 professional women with two children in Shanghai, we examined their work–life transitions and labor market outcomes. We found that the overarching constraints the interviewees faced included a lack of institutional childcare support, low paternal participation and increased physical and cognitive childcare labor. The women also experienced different constraining and enabling factors, leading to four types of labor market outcomes: enhancement, rebound, interruption and stagnation. Most of the interviewees who experienced career upward mobility after giving birth to a second child were urban singleton daughters who received tremendous parental support. Some participants experienced career interruption due to a lack of social support. The state should ensure family‐friendly work environments and promote paternal participation to reduce women's work–life conflict and address gender inequality.
The Plasmodium falciparum eIK1 kinase (PfeIK1) is central for melatonin synchronization in the human malaria parasite. Melatotosil blocks melatonin action on parasite cell cycle
Authors: Dias Bárbara K.M. , Nakabashi Myna , Alves Marina Rangel Rodrigues , Portella Danielle Pagliaminuto , dos Santos Benedito Matheus , Costa da Silva Almeida Fahyme , Ribeiro Ramira Yuri , Schuck Desiree C. , Jordão Alessandro Kappel , Garcia Celia R.S. 
Published: July 2020

AbstractMelatonin and its indoles derivatives are central in the synchronization of malaria parasites. In this research, we discovered that melatonin is unable to increase the parasitemia in the human malaria Plasmodium falciparum that lacks the kinase PfeIK1. The PfeIK1 knockout strain is a valuable tool in the screening of indol‐related compound that blocks the melatonin effect in wild‐type (WT) parasite development. The assays were performed by using flow cytometry with simultaneous labeling for mitochondria viability with MitoTracker Deep Red and nucleus staining with SYBR Green. We found that Melatotosil leads to an increase in parasitemia in P. falciparum and blocks melatonin effect in the WT parasite. Using microscopy imaging system, we found that Melatotosil at 500 nM is able to induce cytosolic calcium rise in transgenic PfGCaMP3 parasites. On the contrary, the compound Triptiofen blocks P. falciparum cell cycle with IC50 9.76 µM ± 0.6, inhibits melatonin action, and does not lead to a cytosolic calcium rise in PfGCaMP3 parasites. We also found that the synthetic indol‐related compounds arrested parasite cycle for PfeIK1 knockout and (WT) P. falciparum (3D7) in 72 hours culture assays with the IC50 values slighting lower for the WT strain. We concluded that the kinase PfeIK1 is central for melatonin downstream signaling pathways involved in parasite cell cycle progression. More importantly, the indol‐related compounds block its cycle as an upstream essential mechanism for parasite survival. Our data clearly show that this class of compounds emerge as an alternative for the problem of resistance with the classical antimalarials.
Volumetric tumor response assessment is inefficient without overt clinical benefit compared to conventional, manual veterinary response assessment in canine nasal tumors
Authors: Nell Esther , Ober Christopher , Rendahl Aaron , Forrest Lisa , Lawrence Jessica 
Published: July 2020

AbstractAccurate assessment of tumor response to therapy is critical in guiding management of veterinary oncology patients and is most commonly performed using response evaluation criteria in solid tumors criteria. This process can be time consuming and have high intra‐ and interobserver variability. The primary aim of this serial measurements, secondary analysis study was to compare manual linear tumor response assessment to semi‐automated, contoured response assessment in canine nasal tumors. The secondary objective was to determine if tumor measurements or clinical characteristics, such as stage, would correlate to progression‐free interval. Three investigators evaluated paired CT scans of skulls of 22 dogs with nasal tumors obtained prior to and following radiation therapy. The automatically generated tumor volumes were not useful for canine nasal tumors in this study, characterized by poor intraobserver agreement between automatically generated contours and hand‐adjusted contours. The radiologist's manual linear method of determining response evaluation criteria in solid tumors categorization and tumor volume is significantly faster (P < .0001) but significantly underestimates nasal tumor volume (P < .05) when compared to a contour‐based method. Interobserver agreement was greater for volume determination using the contour‐based method when compared to response evaluation criteria in solid tumors categorization utilizing the same method. However, response evaluation criteria in solid tumors categorization and percentage volume change were strongly correlated, providing validity to response evaluation criteria in solid tumors as a rapid method of tumor response assessment for canine nasal tumors. No clinical characteristics or tumor measurements were significantly associated with progression‐free interval.
Preparation and Characterization of Nb-1Zr-0.1C Alloy Suitable for Liquid Metal Coolant Channels of High Temperature Reactors
Authors: Majumdar S. , Kishor J. , Behera A. N. , Vishwanadh B. , Borgohain A. , Kapoor R. , Tewari R. , Kain V. , Krishnan Madangopal , Dey G. K. , Banerjee S. 
Published: July 2020

Abstract
A novel process comprising of aluminothermic coreduction of mixed oxides followed by arc and electron beam melt refining was developed for preparation of Nb-1Zr-0.1C alloy. The parameters of the process were optimized by considering the thermodynamic (heat) and mass balance phenomenon. The ingots of the homogenized alloy produced after electron beam melt consolidation were further extruded into tubes. The alloy was vacuum annealed at 1350–1800 °C to study the stability of Nb2C and Nb(Zr)C carbide precipitates in the microstructure. Compression creep tests conducted at 900 and 1000 °C revealed a stress exponent value of 2 and activation energy of 508 kJ/mol. NbSi2-based coatings were developed on the Nb-1Zr-0.1C alloy tubes using pack siliconizing process. The coated alloy was tested for oxidation at 1250 °C, and corrosion in liquid lead-bismuth eutectic (LBE) alloy at 875 °C for prolonged duration. The silicide-coated alloy showed superior oxidation and LBE corrosion resistance at high temperatures. The alloy was found to be a promising material for coolant channels of high temperature reactors.
A Comparative Study on Severe Accident Phenomena Related to Melt Progression in Sodium Fast Reactors and Pressurized Water Reactors
Authors: Bachrata Andrea , Bertrand Fréderic , Marie Nathalie , Serre Fréderic 
Published: July 2020

Abstract
The nuclear safety approach has to cover accident sequences involving core degradation in order to develop reliable mitigation strategies for both existing and future reactors. In particular, the long-term stabilization of the degraded core materials and their coolability has to be ensured after a severe accident. This paper focuses on severe accident phenomena in pressurized water reactors (PWR) compared to those potentially occurring in future GenIV-type sodium fast reactors (SFR). First, the two considered reactor concepts are introduced by focusing on safety aspects. The severe accident scenarios leading to core melting are presented and the initiating events are highlighted. This paper focuses on in-vessel severe accident phenomena, including the chronology of core damage, major changes in the core configuration and molten core progression. Regarding the mitigation means, the in-vessel retention phenomena and the core catcher characteristics are reviewed for these different nuclear generation concepts (II, III, and IV). A comparison between the PWR and SFR severe accident evolution is provided as well as the relation between governing physical parameters and the adopted mitigation provisions for each reactor concept. Finally, it is highlighted how the robustness of the safety demonstration is established by means of a combined probabilistic and deterministic approach.
Cross-Cultural Adaptation and Psychometric Evaluation of the Community Healthy Activities Model Program for Seniors Physical Activity Questionnaire in Chinese Older Adults
Authors: Shi Xiaoyang , Wang Yan , Huang Xiuxiu , Gao Shangshang , Wan Qiaoqin , Shang Shaomei 
Published: July 2020

The study’s aims were to translate the Community Healthy Activities Model Program for Seniors (CHAMPS) questionnaire to Chinese and examine its psychometric properties. Adapting it for use in China involved forward translation, synthesis, back translation, expert review, and pretesting. A convenience sample of 201 Chinese older adults completed the Chinese version (CHAMPS-C) to evaluate its construct validity index and associations with physiological, psychosocial, and energy expenditure measures. The construct validity index of the CHAMPS-C was 0.95, and it had fair to moderate associations with physiological and psychosocial measures, other scales of physical activity, and accelerometer measurements. Our structured, stepwise process of cross-cultural adaptation produced a scale (i.e., CHAMPS-C), with items equivalent in meaning to the English version, for use with Chinese older adults. The findings of this study indicate that the CHAMPS-C has acceptable reliability and validity to assess the physical activity of older Chinese adults.
Different effect of media opacity on automated and manual measurement of foveal avascular zone of optical coherence tomography angiographies
Authors: Zhang Jinyu , Tang Fang Yao , Cheung Carol , Chen Xiaolin , Chen Haoyu 
Published: July 2020

BackgroundOptical coherence tomography angiography (OCTA) provides not only visualisation but also quantitative measurement of foveal avascular zone (FAZ). Media opacity is common in elderly subjects with cataracts. This study aimed to investigate the impact of media opacity on automated and manual FAZ measurement.MethodsCirrus 5000 OCTA and Triton OCTA were used to image FAZ using a 3×3 mm scanning protocol from 30 eyes of 30 healthy normal subjects. Media opacity was simulated with neutral-density filters (optical density (OD): 0.10–0.48 in Cirrus 5000 and 0.15–0.51 in Triton). Signal strength (SS) and signal strength intensity (SSI) were provided by the built-in software in Cirrus 5000 and Triton, respectively. FAZ area, perimeter and circularity were measured automatically using the built-in software as well as a customised MATLAB program. FAZ metrics were also measured manually. The correlations between the OD, SS/SSI and FAZ metrics were analysed using Spearman correlation.ResultsIncreased OD significantly correlated with decreased SS/SSI (rs=−0.602 and −0.925, respectively, both p<0.001), decreased automated FAZ area (rs=−0.344 and −0.766, respectively, both p<0.001), but increased manual FAZ area in both Cirrus 5000 and Triton (rs=0.423 and 0.543, respectively, both p<0.001). Similar results were found for FAZ perimeter and circularity. There was a positive correlation between SS/SSI with the automated FAZ area but negative correlation with the manual FAZ area.ConclusionsThe effect of media opacity on quantitative measurement of FAZ is different between automated and manual measurements. Cautions must be taken when interpreting FAZ measurement in eyes with media opacity.