bluuebunny/crossref_metadata_2025_split huggingface dataset:
Equine granulosa cell tumours among other ovarian conditions: Diagnostic challenges
Authors: Renaudin Catherine D. , Kelleman Audrey A. , Keel Kevin , McCracken Jaye L. , Ball Barry A. , Ferris Ryan A. , McCue Patrick M. , Dujovne Ghislaine , Conley Alan J. 
Published: May 2020

AbstractBackgroundGranulosa cell tumours (GCT) are the most common ovarian tumours in mares. While the classical presentation may not represent diagnostic challenges, diagnosis is not easy in the early stages.ObjectivesIllustrate the variability in the presentation and serum biomarkers associated with ovarian abnormalities in the mare.Study designRetrospective case series.MethodsNonclassical cases of GCTs and other ovarian conditions were identified and behaviour, GCT endocrine results, palpation and ultrasonographic findings are described and the diagnostic value of each is discussed.ResultsMares in this case series with GCTs had been presenting clinical signs ranging from no behavioural changes to behaviours including aggression, stallion‐like and inability to work under saddle. Hormonal profiles of endocrinologically functional GCTs can be erratic and unpredictable. The clinical form and ultrasonographic appearance may also vary with time from an initially enlarged/anovulatory follicular structure that later develops a multicystic ‘honeycomb’ appearance. Mares with GCTs can also present with persistent anovulatory follicles or apparent luteal tissue that are unresponsive to treatment. If both ovaries are of relatively normal size and symmetry, but hormonal biomarkers are markedly increased (AMH >10 ng/mL, inhibin B and/or testosterone >100 pg/mL; 0.37 nmol/L), it is likely that a functional GCT is present. Still, it can be a challenge to decide which ovary to remove. Post‐surgical endocrine testing can be helpful, especially if histopathology is not performed or a GCT is not found.Main limitationsCases limited to 14.ConclusionsGranulosa cell tumours present with a wide variety of clinical signs that do not fit what is commonly described as ‘classic’. Only if AMH, testosterone and inhibin B concentrations are markedly increased, and there is an abnormally enlarged ovary, the diagnosis of a GCT is more confident. In the presence of normal size ovaries, normal hormonal biomarkers and abnormal behaviour, it is more likely that the ovaries are not involved.
Shading effects on canopy and tillering characteristics of continuously stocked palisadegrass in a silvopastoral system in the Amazon biome
Authors: Gomes Fagner Junior , Pedreira Bruno Carneiro , Santos Patrícia Menezes , Bosi Cristiam , Pedreira Carlos Guilherme Silveira 
Published: May 2020

AbstractIn silvopastoral (SP) systems, forage responses depend on the microenvironment in which the plants develop. Our objective was to evaluate canopy and tillering characteristics of shaded 'Marandu' palisadegrass [Brachiaria brizantha (Hochst A Rich) Stapf, syn. Urochloa brizantha] under continuous stocking in a SP system. Treatments were one full sun (FS) and three shaded systems (silvopasture, SP) corresponding to distances from tree groves: 7.5 m north (SP1), and 15 m (SP2) and 7.5 m south (SP3) studied during two rainy seasons (Year 1 and Year 2). The tree in the SP system was Eucalyptus urograndis (hybrid of Eucalyptus grandis W. Hill ex Maiden × Eucalyptus urophylla S. T. Blake). The photosynthetic active radiation was greater in FS (923 μmol m-2 s-1), followed by SP2 (811 μmol m-2 s-1), SP1 (727 μmol m-2 s-1) and SP3 (673 μmol m-2 s-1). Forage accumulation in FS was 15% greater than the mean of SP1, SP2 and SP3 (10,663 kg DM/ha). There was no difference in net accumulation of leaf, stem and dead material, averaging 3,302, 3,420 and 4,063 kg DM/ha respectively. Leaf accumulation and accumulation rate were greater in Year 2, and leaf accumulation rate was similar among treatments (19 kg DM ha−1 day−1). Leaf proportion increased 14% from Year 1 to Year 2. Specific leaf area was greater for treatments SP1 and SP3 (193 cm2/g). Tiller population density was similar across treatments in Year 1. Shaded palisadegrass maintains leaf productivity similar to FS under continuous stocking in an SP system.
Preparation of grape seed polypeptide and its calcium chelate with determination of calcium bioaccessibility and structural characterisation
Authors: Jiang Yuhan , Li Jing , Zhao Huan , Zhao Runtian , Xu Yi , Lyu Xiaoling 
Published: May 2020

SummaryGrape seed polypeptide (GSPP) was obtained by liquid fermentation with Bacillus subtilis TK15.015 from waste grape seed pomace. Its chelation ability with calcium ions was excellent. Simulation of in vitro gastrointestinal digestion showed that GSPP and calcium chelate (GSPP‐Ca) slowly released calcium and decreased stomach stimulation. The solubility and transmission rate of GSPP‐Ca after digestion in the gastrointestinal tract for 8 h maintained high levels of 74.4% and 46.4%, respectively, which far exceeded the levels achieved by other calcium supplements. Structural characterisation showed that calcium ions mainly react with binding sites present in peptides such as carboxyl oxygen, hydroxyl oxygen, amino nitrogen and amide bonds. GSPP‐Ca may be a state in which a calcium‐encapsulating complex formed, leaving calcium ions in a soluble state, suggesting its possible use as a high‐quality calcium supplement.
Amino acid, structure and antioxidant properties of Haematococcus pluvialis protein hydrolysates produced by different proteases
Authors: Zhu Yunping , Zhao Xiaoyan , Zhang Xiaowei , Liu Hongkai , Ao Qiang 
Published: May 2020

SummaryThe impact of different protease hydrolysis on the amino acid, structure and antioxidant properties of H. pluvialis protein (HP) was investigated. Results showed that the hydrolysate obtained by Alcalase exhibited the highest degree of hydrolysis (20.59%) and peptide yield (92.64%). The essential amino acid, hydrophobic, sulphur and aromatic amino acid contents of enzyme hydrolysates were significantly higher than HP (P < 0.05). FTIR spectra showed that the β‐sheet proportion of HP hydrolysates were higher compared with HP, the proportion of random coil structure was lower. The α‐helix content of the hydrolysate obtained by Alcalase was the highest, while the turn proportion was the lowest. The Trypsin derived hydrolysate presented the best DPPH and ABTS scavenging ability, and ferric reducing antioxidant power than other HPHs. These results suggested that HP hydrolysates have a great potential as natural functional ingredients in food manufacture.
Effect of particle size on flow behaviour and physical properties of semi‐ripe plantain (AAB Musa spp) powders
Authors: Irie Kady Rosine , Petit Jérémy , Gnagne Eliane Hadiowe , Kouadio Olivier Kouadio , Gaiani Claire , Scher Joël , Amani Georges N’Guessan 
Published: May 2020

SummaryThis work was conducted to study the influence of particle size and shape on the flow behaviour of semi‐ripe plantain powders. An improved plantain variety (Big Ebanga), two hybrids (FHIA 21 and PITA 3), and a local variety (Corne 1, as reference material) were powdered by successive drying, milling, and sieving into three granulometric classes: unsieved (<500 µm), fine fraction (<180 µm; class 1), and coarse fraction (between 180 and 500 µm; class 2), thus differing in particle size distribution. Physical properties (water activity, particle size distribution, shape factors) were characterised and flow properties (basic flowability energy, compressibility, fluidisability, cohesion, etc.) were investigated using the FT4 powder rheometer. Convexity of unsieved and fine powders was slightly higher than for coarse powders. Fine powders of all plantain varieties were found to have the lowest water activity and the lowest flowability in unconfined conditions. Unsieved and fine powders presented a fluidised state at the end of the FT4 standard aeration test and their minimum fluidisation velocity was low, confirming the good fluidisability of these powders.
Clarifying the Dominant Logic Construct by Disentangling and Reassembling its Dimensions
Authors: Engelmann Alexander , Kump Barbara , Schweiger Christina 
Published: May 2020

AbstractSince its introduction, Prahalad and Bettis's concept of dominant logic has informed a variety of scholarly conversations in management and strategy research. However, scholars have interpreted dominant logic in different ways, emphasizing different aspects, such as managerial mindsets, administrative tools and management functions, as defining elements. Similarly, empirical studies have captured various aspects, such as meanings of entrepreneurs, observable strategic decisions and business model similarity, as indicators of dominant logic. Consequently, the concept lacks analytical clarity, and it is difficult to compare or generalize findings from this diverse set of studies. The aim of this review is to improve conceptual clarity by analysing, comparing and evaluating the existing interpretations and assessments of dominant logic in 94 studies. In the first part of the review, by disentangling the interpretations of the concept, we show that dominant logic consists of four defining dimensions: (i) shared mental models; (ii) values and premises; (iii) organizational practices; and (iv) organizing structures. In the second part, we reassemble dominant logic into an integrative model and theorize about how these dimensions operate in concert to produce a firm's dominant logic. Thus, our main contribution is a clarification and synthesis of the literature, which comes with implications on how future research can conceptualize and operationalize dominant logic more consistently.
Incorporating the Clinical Frailty Scale into routine outpatient nephrology practice: an observational study of feasibility and associations
Authors: Kumarasinghe Anuttara Panchali , Chakera Aron , Chan Kien , Dogra Sharan , Broers Sally , Maher Sean , Inderjeeth Charles , Jacques Angela 
Published: May 2020

AbstractBackgroundThere is an unmet need for routine and accurate prognostication of older adults with end‐stage kidney disease (ESKD) and subsequently inadequate advance care planning. Frailty, a clinical syndrome of increased vulnerability, is predictive of adverse health outcomes in the renal population. We propose the Clinical Frailty Scale (CFS) as a feasible tool for routine use in the nephrology outpatient setting to address this unmet need.AimsTo assess feasibility and associations of incorporating CFS assessment into routine outpatient nephrology practice in the pre‐dialysis setting.MethodsCFS was integrated into the outpatient nephrology clinic proforma. A convenience sample of 138 patients aged >50 years, with estimated glomerular filtration rate (eGFR) <30 mL/min/1.73 m2, attending the outpatient service between September 2018 and April 2019 was included.ResultsEighty‐one CFS assessments were completed by nephrologists, nephrology advanced trainees and clinical nurse specialists. CFS completion rates were 79% from the multidisciplinary Low Clearance Clinic and 41% from nurse‐led Pre‐dialysis Education Clinic.Planned modality of ESKD management varied with degree of frailty (P < 0.001). 21% of patients who had CFS completed were planned for Conservative Management of ESKD, in contrast to only 5% of those who did not have CFS assessment completed (P < 0.001).ConclusionFrailty assessment via CFS was feasible in outpatient practice when integrated into routine clinical assessment in a dedicated clinic.Planned ESKD management varied with the degree of frailty. Completion of frailty assessment, when compared with non‐completion, appears to be associated with increased planned conservative management of ESKD.
Cryopreservation of lipoaspirates: in vitro measurement of the viability of adipose‐derived stem cell and lipid peroxidation
Authors: Kim Dong Yeon , Kim Eunjin , Kim Ki Joo , Jun Young‐Joon , Rhie Jong‐Won 
Published: May 2020

AbstractAs the storage time of the fat tissue passes by, lipid peroxidation and creation of by‐products may take place. The objective of this study was to evaluate the cell viability and functional changes of adipose‐derived stem cells (ADSCs) in the cryopreserved lipoaspirates at different temperatures in accordance with lipid peroxidation. Lipoaspirates acquired from liposuction were divided into four different temperature groups and stored at 4°C, −20°C, −80°C, and −196°C. After isolating ADSC from each sample, gross cell morphology and cell viability were compared with doubling time and colony‐forming unit (CFU) formation ability. Acid value, that is, thiobarbituric acid value was measured to assess lipid peroxidation. No viable ADSC was observed in −20°C and −196°C samples for past 1 week and a superior number of the live cells were detected in the 4°C group compared with the −80°C group. However, the persistence of cell division and CFU formation after 1 week was only observed in adipocytes stored at −80°C. Lipid peroxidation mainly occurred at 4°C and −20°C storage samples. If the lipoaspirates were planned to be cryopreserved, it is advised to store at −80°C. However, the number of actually functional ADSCs is very low. Furthermore, even in the cryopreserved status, continuous lipid peroxidation and by‐product creation took place, suggesting shorter preservation period as possible in the clinics.
Facilitators and barriers of using digital technology for the management of diabetic foot ulcers: A qualitative systematic review
Authors: Foong Hui Foh , Kyaw Bhone Myint , Upton Zee , Tudor Car Lorainne 
Published: May 2020

AbstractThe use of digital technology has been shown to be effective in managing chronic conditions. Telemedicine and mobile application are two common applications of digital technology in managing diabetic foot ulcers (DFU). The facilitators and barriers of using it for DFU management are yet to be explored. This is a qualitative systematic review. Five bibliography databases and grey literature sources were searched (2000‐2019). Two reviewers independently screened the citations, extracted the data, assessed the quality of the included studies, and performed thematic synthesis. Three studies on patients and five studies on healthcare practitioners (HCPs) were included. Two studies focused on the use of mobile applications and six on telemedicine. In studies on patients, four analytical themes were generated: the relationships with HCPs; the attitude towards the usage of digital technology; the role of wound image taking; and impact of digital technology on DFU care, encompassing 15 facilitators (eg, enabling community support, improving wound care knowledge) and 12 barriers (eg, lack of technological savviness, difficulty reading on smartphones). Three analytical themes were generated from studies on HCPs: the impact of digital technology on HCPs; the role of digital technology in DFU care; and organisation of DFU care delivery, encompassing 17 facilitators (eg, adequate wound care training, digital technology enables holistic care) and 16 barriers (eg, lack of multidisciplinary approach in caring for DFU, lack of direct contact in care provision). Patients and HCPs reported various barriers and facilitators relating to different aspects of using digital technology in DFU management. Our findings can help inform future research as well as the adoption of digital technology in DFU management.
Identification of two novel mutations in POU4F3 gene associated with autosomal dominant hearing loss in Chinese families
Authors: Bai Xiaohui , Zhang Fengguo , Xiao Yun , Jin Yu , Zheng Qingyin , Wang Haibo , Xu Lei 
Published: May 2020

AbstractAutosomal dominant non‐syndromic hearing loss is genetically heterogeneous with 47 genes identified to date, including POU4F3. In this study, by using a next‐generation sequencing panel targeting 127 deafness genes, we identified a pathogenic frameshift mutation c.704_705del and a missense mutation c.593G>A in two three‐generation Chinese families with late‐onset progressive ADNSHL, respectively. The novel mutations of POU4F3 co‐segregated with the deafness phenotype in these two families. c.704_705del caused a frameshift p.T235fs and c.593G>A caused an amino acid substitution of p.R198H. Both mutations led to an abnormal and incomplete protein structure. POU4F3 with either of the two mutations was transiently transfected into HEI‐OC1 and HEK 293 cell lines and immunofluorescence assay was performed to investigate the subcellular localization of mutated protein. The results indicated that both c.704_705del (p.T235fs) and c.593G>A (p.R198H) could impair the nuclear localization function of POU4F3. The p.R198H POU4F3 protein was detected as a weak band of the correct molecular weight, indicating that the stability of p.R198H POU4F3 differed from that of the wild‐type protein. While, the p.T235fs POU4F3 protein was expressed with a smaller molecular weight, implying this mutation result in a frameshift and premature termination of the POU4F3 protein. In summary, we report two novel mutations of POU4F3 associated with progressive ADNSHL and explored their effects on POU4F3 nuclear localization. These findings expanded the mutation spectrum of POU4F3 and provided new knowledge for the pathogenesis of POU4F3 in hearing loss.
Implementation of Hip Fracture Co‐Management Program (AGS CoCare: Ortho®) in a Large Health System
Authors: Sinvani Liron , Goldin Mark , Roofeh Regina , Idriss Nayla , Goldman Ariel , Klein Zachary , Mendelson Daniel Ari , Carney Maria Torroella 
Published: May 2020

OBJECTIVESAcute hip fracture is common and leads to significant morbidity and mortality. Co‐management programs, such as American Geriatric Society (AGS) CoCare: Ortho®, that optimize perioperative care of older adults, have demonstrated improved outcomes. Yet there is substantial variation in hip fracture care nationally. Our objective was to describe the implementation of AGS CoCare: Ortho® across a large integrated health system.DESIGNProgram implementation of four phases.SETTINGLarge integrated health system.PARTICIPANTSOne tertiary and three community hospitals.MEASUREMENTSThe first two phases were communication and system‐level planning. The communication phase consisted of getting health system leadership buy‐in, creating an interdisciplinary steering committee, and building a business model. The planning phase consisted of choosing process and outcome measures, ensuring accurate and timely data collection, and creating standardized order sets and physician documentation.RESULTSThe second two phases were hospital‐level planning and implementation. The planning phase consisted of identifying sites and developing the co‐management structure. The implementation phase consisted of identifying and engaging frontline staff, rolling out the program, optimizing workflow, and educating providers.CONCLUSIONThe program was implemented at four diverse sites. Major lessons learned included the need for an engaged steering committee to oversee the program; the importance of standardizing order sets and documentation; the utilization of hospitalists as co‐managers; the benefit of developing and actively using a data dashboard; the challenge of ensuring wide uptake of education modules; and the need to take proactive steps to improve multidisciplinary communication. J Am Geriatr Soc 68:1706‐1713, 2020.
Nutritional status, food choices, barriers and facilitators to healthy nutrition in pregnant women with severe mental illness: a mixed methods approach
Authors: Frayne J. , Hauck Y. , Sivakumar P. , Nguyen T. , Liira H. , Morgan V. A. 
Published: May 2020

AbstractBackgroundAlthough widely acknowledged that adequate maternal nutrition is important for mother and baby, limited research has focussed on women with severe mental illnesses (SMI) in pregnancy. The present study reports on nutritional factors and food choices and investigates barriers and facilitators to healthy nutritional choices by pregnant women with SMI.MethodsA prospective mixed method study was undertaken of 38 pregnant women with SMI, including a cross‐sectional survey, a food frequency questionnaire and 12 postnatal qualitative interviews, with integrated analysis of all data.ResultsElevated rates of obesity (35%) were found, with 82% of women having above the recommended gestational weight gain. Despite perceived knowledge, 32% of women did not meet any of the Five Food Group serving recommendations for pregnancy and consumed above‐recommended levels for processed (19%) and sugar snacks (51%). Thematic analysis identified four main barriers: a discrepancy between knowledge and action, food cravings, mental health, and physical health. During pregnancy, food cravings were reported in 66% of women, psychological distress in 71% and physical distress in 37%. Screening identified 19% with potential eating disordered behaviours. Despite the challenges, several facilitators were identified and covered three themes: access to a dietitian, information delivery and support, and comprehensive care.DiscussionWomen with SMI in pregnancy struggle with issues of obesity, gestational weight gain, food cravings and possible eating disorder behaviours. They have additional challenges when pregnant, with management of their mental health and physical health having a direct impact. Interventional strategies in this population should incorporate findings from this research.
Advances in therapeutic vaccines for treating human papillomavirus‐related cervical intraepithelial neoplasia
Authors: Barra Fabio , Della Corte Luigi , Noberasco Giovanni , Foreste Virginia , Riemma Gaetano , Di Filippo Claudia , Bifulco Giuseppe , Orsi Andrea , Icardi Giancarlo , Ferrero Simone 
Published: May 2020

AbstractAimHuman papillomavirus (HPV) is the etiologic agent of the majority of cervical intraepithelial lesions (CIN) and cervical cancers. While prophylactic HPV vaccines prevent infections from the main high‐risk HPV types associated with cervical cancer, alternative nonsurgical and nonablative therapeutics to treat HPV infection and preinvasive HPV diseases have been experimentally investigated. Therapeutic vaccines are an emerging investigational strategy. This review aims to introduce the results of the main clinical trials on the use of therapeutic vaccines for treating HPV infection and ‐related CIN, reporting the ongoing studies on this field.MethodsData research was conducted using MEDLINE, EMBASE, Web of Sciences, Scopus, ClinicalTrial.gov, OVID and Cochrane Library querying for all articles related to therapeutic vaccines for the treatment of HPV‐related CIN. Selection criteria included randomized clinical trials, nonrandomized controlled studies and review articles.ResultsPreliminary data are available on the evaluation of therapeutic vaccines for treating cervical HPV infections and CIN. Despite having in vitro demonstrated to obtain humoral and cytotoxic responses, therapeutic vaccines have not yet clinically demonstrated consistent success; moreover, each class of therapeutic vaccines has advantages and limitations. Early clinical data are available in the literature for these compounds, except for MVA E2, which reached the phase III clinical trial status, obtaining positive clinical outcomes.ConclusionDespite promising results, to date many obstacles are still present before hypothesize an introduction in the clinical practice within the next years. Further studies will draw a definitive conclusion on the role of therapeutic vaccines in this setting.