bluuebunny/crossref_metadata_2025_split huggingface dataset:
EBV-miR-BART12 accelerates cancer cell migration and invasion through targeting TPPP1 in EBV associated cancer
Authors: Wu Yingfen , Wei Fang , Wang Dan , Tang Le , Yang Liting , Shi Lei , Guo Can , Xiong Fang , Li Xiayu , Wu Xu , Gong Zhaojian , Zhou Ming , Xiang Bo , Li Xiaoling , Li Guiyuan , Zeng Zhaoyang , Xiong Wei 
Published: December 2019

Abstract
Background: Epstein-Barr virus (EBV) is suggested to actively utilize its EBV microRNAs (miRNAs) to manipulate viral and cellular functions during neoplasia transformation. But its role in tumor development and maintenance remains unclear. The objective of this study is to examine EBV-miRNA-BART12 implicated in EBV associated carcinogenesis. Methods :Expression of EBV-miRNA-BART12 and TPPP1 (Tubulin Polymerization Promoting Protein 1) was confirmed by GEO datasets, RT-PCR and in situ hybridization. Luciferase analyses, RT-PCR and western blotting experiments examined and confirmed that EBV-miRNA-BART12 targeted the 3'-UTR of TPPP1 mRNA. Migration and invasion ability were measured by wound healing and transwell assay. And the mechanism was assessed by western blotting and immunofluorescence. Results : Our results showed that EBV-miRNA-BART12 was highly expressed and TPPP1 was lowly expressed in NPC and/or GC and tightly associated with these patients' prognosis. Additionally, EBV-miR-BART12 advanced the HDAC6 (Histone Deacetylase 6) activity in cells by inhibiting TPPP1, and stimulated acetylation of α-tubulin and β-catenin, thereby increasing the microtubule instability and activating the epithelial-mesenchymal transition (EMT) process. Conclusion : EBV-encoded BART12 promotes migration and invasion of EBV associated NPC and gastric cancer by inhibiting TPPP1 mRNA and promoting the microtubule instability and the cellular EMT process.
Associations between Physical Activity Motives and Trends in Moderate-to-Vigorous Physical Activity Among Adolescents Over Five Years.
Authors: Nader Patrick Abi , Gaudet Jeffrey , Brunet Jennifer , Gunnell Katie E. , Doré Isabelle , Sabiston Catherine M. , Boudreau Jonathan , Bélanger Mathieu 
Published: December 2019

Abstract
BACKGROUNDMotives for participation in physical activity (PA) have been associated with moderate-to-vigorous intensity PA (MVPA); however, these associations have yet to be established during the transition from childhood to adolescence. This study examined the longitudinal associations between five PA motives (i.e., enjoyment, competence, fitness, social, and appearance) and MVPA over a 5-year period spanning late childhood (9-11 years), early adolescence (12-14 years), and middle adolescence (15-16 years). METHODSSelf-report data (n = 937) were drawn from the ongoing Monitoring Activities for Teenagers to Comprehend their Habits study. The Motives for Physical Activity Measure-Revised (MPAM-R) questionnaire was used to measure PA motives. MVPA was assessed with an empirically supported 2-item measure developed specifically for adolescents. Data from three times points per school year over 5 years (resulting in 15 survey cycles) were analyzed using sex-stratified mixed effects regression models after establishing longitudinal measurement invariance for the MPAM-R.RESULTSMVPA increased from cycle 1 (9-11 years) to 7 (11-13 years) for girls and from cycle 1 (9-11 years) to 9 (12-14 years) for boys before decreasing for both sexes through to cycle 15 (14-16 years). Longitudinal measurement invariance was established for a 22-item version of the MPAM-R. For boys, enjoyment and competence motives were positively associated with MVPA from late childhood to middle adolescence and fitness motives were negatively associated with MVPA from late childhood to middle adolescence. For girls, enjoyment motives were positively associated with MVPA during late childhood and competence motives were positively associated with MVPA from late childhood to middle adolescence. CONCLUSIONSPA interventions should aim to increase enjoyment during PA and provide opportunities for children and adolescents to develop a desire to build competence during PA. PA interventions targeted to youth should avoid promoting the desire to be active to improve fitness, particularly among boys.
Complex evolution of porcine endogenous retroviruses
Authors: Chen Yicong , Chen Mingyue , Duan Xiaoyan , Cui Jie 
Published: December 2019

Abstract
Background: Porcine endogenous retroviruses (PERVs) are proviruses that can replicate in human cells. However, the evolutionary process leading to the generation of modern PERVs is not well understood.Results: We mined 14 pig genomes and other available 304 mammalian genomes in silico, which led to the documentation of 185 full-length PERVs. Notably, we found two novel ERVs in the lesser Egyptian jerboa ( Jaculus jaculus ) and rock hyrax ( Procavia capensis ) named ERV-Gamma-Jja and ERV-Gamma-Pca, respectively, which were the source of the modern PERVs. Phylogenetic analyses provided evidence for the multiple origins of PERVs involving hosts of rodents, rock hyrax, and pigs. Conclusion: These new findings help us to understand the complex evolution of the modern PERVs.
Continuous Medical Education among resident physicians in Abha, Saudi Arabia: Current Practice and Existing Gaps
Authors: Almoalwi Najwa , Alsaleem Safar , Siddiqui. Aesha Farheen 
Published: December 2019

Abstract
Background: Continuing Medical Education (CME) is concerned with the maintenance, improvement, and promotion of the health care and exhausts the physicians’ working life-span. Although international research is extensive, only a dearth of studies exists in Saudi Arabia and the Middle East. This study aims to explore CME practices of resident physicians in Abha City and the existing gaps. Method: A cross-sectional questionnaire-based descriptive study conducted on residents training in various health-care facilities in Abha city. Results: Participants in the study were (n=300) doctors, of whom (n = 239, 79.7%) reported attending lectures and seminars for their CME needs followed by (n = 119, 39.7%) who attended case presentations. Electronic CMEs were used by a minority of (n = 24, 8%), and only (n = 82, 27.3%) attended journal clubs. Being busy was the main barrier against self-reading as per (n = 212, 70.7%) of participants, and by (n = 155, 51.7%) against lectures and seminars, and by (n = 124, 41.3%) against courses attendance. There was no significant association between gender, nationality, or training level and satisfaction with CME activities (p = 0.982, p = 0.924, and p = 0.5400 respectively). Satisfaction with CME activities varied considerably across specialties (p = 0.039), psychiatry trainees were far less satisfied than their restorative dentistry counterparts (p = 0.0046), as well as for General Surgery trainees (P = 0.0230), Ophthalmology trainees (p = 0.0301), pediatrics trainees (p = 0.0214), and Preventive Medicine trainees (p = 0.0283). Discussion: The participant physicians favored contact CME activities but not non-contact CME activities. In consistence with global research findings, being busy was the main barrier against CME goals attainment. Participants affirmed that CME activities improved their clinical practice, clinical skills and academic skills. Conclusion: Promotion of online learning in Saudi Arabia. Support of residents with protected time for their CME activities. Encouragement of trainees in to further advice about their preference in terms of CME forms to help boost their engagement
Synergistic In-Vitro Effects through Radiation and Blocking the Epidermal Growth Factor Receptor (EGFR) with the Monoclonal Antibody Cetuximab in Prostate Carcinoma Cell Line DU145
Authors: Schneider Raik , Ochel Hans-Joachim , Neumann Karsten , Jandrig Burkhard , Hass Peter , Wahlke Matthias , Schostak Martin , Brunner Thomas , Christoph Frank , Gademann Guenther 
Published: December 2019

Abstract
Background: The EGF-receptor is often overexpressed in advanced prostate carcinoma. In-vitro studies in prostate carcinoma cell-line DU145 demonstrated increased sensibilities to radiation with Cetuximab; in-vivo effects were not detected. Methods: In vitro, we analyzed the effect of radiation and Cetuximab in cell-lines DU145 and A431 (reference), using a proliferation assay, colony-forming unit assay, and Annexin-V apoptosis assay. We analyzed changes in the protein expression of pEGFR and pERK1/2 post-radiation and Cetuximab. Additionally, we investigated the impact of Cetuximab long-term treatment on the development of secondary-resistance-mutations. Results: DU145 cell counts were reduced by 44% after 4Gy (p=0.006) and by 55% after 4Gy and Cetuximab (p<0.001). The surviving fraction was 0.69 after 2Gy; 0.41, 4Gy; and 0.15, 6Gy (p<0,001). The additional Cetuximab-treatment did not significantly alter the impact on growth reduction or on the surviving fraction. After radiation and Cetuximab-treatment minor effects on the apoptotic cell-fraction in DU145 were detected. Using western blot, there were no pEGFR and pERK1/2 protein signals after Cetuximab-treatment. While no mutations of RAS, BRAF, PI3KCA and no amplifications of HER2 were detected, there were a TP53 mutation before and after long-term treatment with Cetuximab. Conclusion: Radiation inhibits cell-proliferation and colony-growth and induces apoptosis in DU145. Despite blocking EGFR-MAP-Kinase pathway with Cetuximab, no significant radiation-sensitizing-effect was detected. Cetuximab-treatment did not cause typical resistance mutations in DU145. Further research must clarify whether a combination of anti-EGFR therapeutics and immune-oncological approaches can increase the radiation-sensitizing-effect.
Two novel gene-specific markers at pik locus facilitate the application of rice blast resistant alleles in breeding
Authors: Tian Dagang , Chen Ziqiang , Lin Yan , Chen Zaijie , Luo Jiami , Wang Mo , Chen Songbiao , Ji Pingsheng Ji , Yang Liming , Wang Zhonghua , Wang Feng 
Published: December 2019

Abstract
Background: Rice blast disease, caused by Magnaporthe oryzae, is a major constraint for rice production in the world. Introgression of blast-durable resistance genes into high-yielding rice cultivars has been considered an agricultural priority in an effort to control the disease. The blast resistance Pik locus, located on chromosome 11, contains at least six important resistance genes, but these genes have not been widely employed in resistance breeding since existing markers hardly satisfy current breeding needs owing to their limited scope of application.Results: In the present study, two PCR-based markers, Pikp-Del and Pi1-In, were developed to target the specific InDel (insertion/deletion) of the Pik-p and Pi-1 genes, respectively. The two markers precisely distinguished Pik-p, Pi-1, and the K-type alleles at the Pik locus, which is a necessary element for functional genes from rice varieties. Conclusions:Two gene-specific markers of Pi-kp and Pi1 identified that only several old varieties contain the two genes, nearly half these varieties yet carry the K-type alleles. Therefore, these identified varieties can be new gene sources for developing blast resistant rice. The two newly developed markers should be highly useful for using Pi-kp, Pi1 and other resistance genes at the Pik locus in marker-assisted selection (MAS) breeding programs.
Impact of risk assessment on the demand for dental services in Primary Health Care
Authors: Ramos Danielle Viana Ribeiro , Monteiro Camila Nascimento , Borchardt Danielle , Tribis Leonardo , Sanches Thais Paragis , Bonfim Daiana , Palacio Danielle da Costa , Sousa Maria da Luz Rosário , Mota Marília Jesus Batista de Brito 
Published: December 2019

Abstract
Background: The access to dental health services is a big problem in many parts of the world. The Primary Health Care proposes the organization of the access with equity and it can be done by stratification of risk and scale of vulnerabilities. The aim was to evaluate the impact of the implementation of risk classification tools to improve the access to dental health demand in Primary Health Care and to evaluate the effectiveness of implantation of the tool: risk classification and Coelho and Savassi’s scale for organization of dental health service demand in one Primary Health Service in São Paulo city.Method: This was a cross-sectional study in which medical records (n = 1215) were evaluated before and after the implementation of the tool. The outcomes were 'immediate care', 'first dental appointment' and 'treatment completed', the independent variables were risk classification, Coelho and Savassi’s scale, caries risk, periodontal disease, soft tissue, age and gender. Descriptive analyzes were performed comparing the variables of the study before and after the implantation of the tools (risk classification and ”Coelho and Savassi” scale). Logistic Regression models and chi-square tests were performed to analyze these associations.Results: The most prevalent age group was 20 to 59 years old, 62.2% before and 59.4% after implantation. The main complaint of dental demand was pain. Regarding the “Coelho and Savassi” scale”, 87.1% presented low social risk (R1), 8.5% medium risk (R2), 4.2% high risk (R3). The immediate care before implantation was 9.4% (n=56) and 39.9% (n=246) after implantation (p <0.001). The first dental appointment was 20.7% before implantation and 34.9% after the implantation (p <0.001). Regarding the treatment completed, there was an increase from 10% to 14.1% after the implantation of the tools (p = 0.001).Conclusions: The implementation of the tools ‘risk classification and ”Coelho and Savassi” scale’ had impact in organization of odontological demand, with indicated an increase in the number of visits on the same day, first dental appointments and completed treatments. These results reinforce the importance of use of the tools to organize oral health care access.
CPI INFLATION IN AFRICA: FRACTIONAL PERSISTENCE, MEAN REVERSION AND NONLINEARITY
Authors: Yaya O. S. , Akintande O. J. , Ogbonna A. E. , Adegoke H. M. 
Published: December 2019

Abstract
Price stability has been one of the key mandates that apex monetary authorities strive to achieve globally. While most developed economies have achieved single digit inflation rates, most developing economies, especially African countries still experience alarming double-digit inflation rates. This paper therefore examined the dynamics of inflation in sixteen African countries. We employed the fractional persistence framework with linear trend and non-linear specifications based on Chebyshev’s polynomial in time. The results indicated nonlinear time trend in inflation for most of the countries. With the exception of Burkina Faso, which exhibited plausibility of naturally reverting to its mean level, the majority of the selected African countries would require stronger interventions to revert their observed inflationary levels to their mean levels.
Successful Reduction of Blood Culture Contamination in an Emergency Department by Monitoring and Feedback
Authors: Al-Hamad Arif M. 
Published: December 2019

Background:Blood Culture (BC) contamination is a common problem in the Emergency Department (ED) and is associated with prolonged length of patient stay and excess costs.Objective:The study aimed to investigate the impact of monthly monitoring and feedback of BC results on contamination rates.Methods:Data from a previous study showed that the contamination rate in the ED consistently exceeded the recommended level. This triggered an ad hoc Quality Improvement team to develop and implement a corrective action plan. In 2017, BC contamination rates were reported to the ED on a monthly basis. In response to this, ED staff conducted intensified educational workshops, followed by private counselling and competency assessment of nurses who collected contaminated BCs.Results:A total of 12 educational workshops were conducted in February and March, 2017. The intervention resulted in >60% reduction in the contamination rate, from 8.6% baseline level to less than 3%. Of the 2660 BC sets drawn in 2017 from 1318 patients, 128 (4.8%) were contaminated, accounting for 39.5% of the total number of positive cultures. Sixty percent of the contaminated BCs grew Coagulase-negativeStaphylococcusspecies; other contaminants includedCorynebacteriumspp.,Micrococcusspp.,Propionibacteriumspp.,viridansStreptococcus, andNeisseriaspp.Conclusion:Continuous monitoring and feedback of contamination rates reduced BC contamination.
Propiedades materiales e índices de rendimiento acústico de veintidós maderas mexicanas. Determinación por ultrasonido.
Authors: Sotomayor Castellanos Javier Ramón , Villaseñor Aguilar José María 
Published: December 2019

El objetivo de la investigación fue determinar las propiedades materiales (densidad aparente, velocidad del ultrasonido, módulo dinámico y coeficiente de amortiguamiento) y los índices de rendimiento acústico (coeficiente de radiación acústica, coeficiente de eficiencia acústica, índice de vibración acústica e índice de anti-vibración acústica) de la madera de 22 especies mexicanas, con el fin de evaluar su potencial uso en aplicaciones acústicas, como lo es la fabricación de instrumentos musicales en general, y en particular, los de cuerda. Se encontró que las propiedades materiales y los índices de rendimiento acústico de las veintidós maderas estudiadas, son características de cada una de ellas. La velocidad del ultrasonido no presenta una relación lineal con la densidad aparente de la madera. En cambio, el módulo dinámico es proporcional a la densidad aparente y las maderas de baja densidad aparente presentan mejores coeficientes de eficiencia acústica en comparación con las especies de altas densidades. Se concluye que un criterio de selección para una madera con aptitudes para ser utilizada en la fabricación de instrumentos musicales es la combinación de una baja densidad aparente, un alto módulo dinámico y un bajo coeficiente de amortiguamiento.
Against Anarchy and Hitler: French Nationalism and Spanish Civil War
Authors: Molodiakov V. E. 
Published: December 2019

Combination of internal political and social crisis with armed conflict in the neighbour country behind the less dangerous frontier without any possibility of obtaining fastly any real aid from allies is one of the worst possible political scenarios in the time of peace. France faced such a situation in 1936 after her Popular Front’s electoral victory and the beginnig of military mutiny in Spain provoqued by further escalation of internal political struggle. Mutiny developed into civil war that, beeing local geographically, became a global political problem because it troubled many great powers and first of all France. This article depicts and analyzes position and views on Spanish civil war and its antecedents of French nationalist royalist movement «Action française» leaded by Charles Maurras (1868–1952) and her allies in next generations of French nationalists – philosopher and political writer Henri Massis (1886–1970) and novelist Robert Brasillach (1909–1945). All of them from the first day hailed Spanish Nationalist cause and were sure in her final victory so took side against any French help, first of all military, to Spanish Republican government, propagated Franco’s political program, denounced Soviet intervention into Spanish affairs and “Communist threat”. Staying for Catholic and Latin unity French nationalists were anxious to prevent Franco’s rapprochement with Nazi Germany that they regarded as France’s “hereditary emeny” notwithstanding of political regime. Trips of Maurras and Massis to Spain in 1938 and theirs meetings with Franco were aimed to demonstrate this kind of unity with silent but clear anti-German overtone. Brasillach’s “History of War in Spain” (1939) became the first French overview of the events from Nationalist point of view.
PENERAPAN MODEL PEMBELAJARAN INKUIRI TERBIMBING UNTUK MENINGKATKAN HASIL BELAJAR IPA SISWA KELAS V SDIT RAUDHATURRAHMAH PEKANBARU
Authors: Purnamasari Ayu 
Published: December 2019

Penelitian ini bertujuan untuk meningkatkan hasil belajar IPA siswa melalui model pembelajaran inkuiri terbimbing pada siswa Kelas V SDIT Raudhaturrahmah Pekanbaru Tahun Pelajaran 2017/2018. Penelitian ini dilaksanakan pada bulan Maret sampai bulan April 2018. Dengan jumlah siswa 30 orang, 14 orang siswa laki-laki, dan 16 orang siswa perempuan. Desain penelitian ini adalah Penelitian Tindakan Kelas (PTK). Parameter yang diukur adalah, hasil belajar siswa, aktivitas siswa, dan aktivitas guru. Sedangkan instrumen penelitian yang digunakan adalah lembar butir soal dan lembar observasi aktivitas siswa dan aktivitas guru. Hasil observasi siklus I pertemuan pertama menunjukkan aktivitas guru sudah cukup (55%), pertemuan kedua (65%) kategori baik dan pemuan ketiga baik sekali (85%). Untuk siklus II observasi aktivitas guru meningkat dengan kategori amat baik sekali (85%) dan sesuai dengan perencanaan, untuk aktivitas siswa juga meningkat dengan kategori baik sekali (95%). Persentase aktivitas siswa dalam melaksanakan pembelajaran pada siklus I pertemuan 1 yaitu 50% kategori cukup dan pertemuan ke 2 yaitu 60% kategori cukup, dan pertemuan tiga 85% kategori baik sekali. Sedangkan pada siklus II pertemuan 1 yaitu 85% kategori baik sekali dan pertemuan ke 2 siklus II 95% kategori baik sekali. Berdasarkan hasil analisis data dari pembahasan, nilai rata-rata skor dasar siswa sebelum penerapan model pembelajaran inkuiri terbimbing adalah 59.83 kemudian meningkat menjadi 72,17 pada siklus I dan mengalami peningkatan kembali pada siklus II menjadi 80,17. Dengan demikian dapat disimpulkan bahwa dengan penerapan model pembelajaran inkuiri terbimbing dapat meningkatkan hasil belajar IPA siswa Kelas V SDIT Raudhaturrahmah Pekanbaru.
Penggunaan Gambar Struktur Organisasi Kabupaten/Kota untuk Meningkatkan Hasil Belajar Pendidikan Kewarganegaraan pada Siswa
Authors: Jamuir Jamuir 
Published: December 2019

Penelitian ini dilaksanakan di SD Negeri 010 Lubuk Keranji Kecamatan Bandar Petalangan Kabupaten Pelalawan dimulai dari tanggal 25 september 2018 sampai tanggal 02 Oktober 2018. Adapun yang menjadi subjek dalam penelitian perbaiakan pembelajaran ini adalah siswa kelas IV dengan jumlah siswa 15 orang, dengan 8 orang siswa laki-laki dan 7 orang siswa perempuan. Penelitian ini dikhususkan pada mata pelajaran Pendidikan Kewarganegaraan. Pada mata pelajaran Pendidikan Kewarganegaraan yaitu sebelum dilakukan tindakan data awal ketuntasan hasil belajar siswa hanya mencapai 46,6%, atau 7 orang dari 15 siswa, dan setelah dilakukan tindakan pada siklus pertama meningkat mencapai 66,6% atau 10 orang dari 15 siswa setelah siklsu ke II jauh meningkat lagi dengan ketuntasan mencapai 100% atau keseluruhan siswa. Berdasarkan hasil penelitian dan analisa yang telah diuraikan di atas, maka dapat disimpulkan bahwa perbaikan pembelajaran pada mata pelajaran Pendidikan Kewarganegaraan Penerapan strategi pembelajaran dengan menggunakan Media Gambar struktur organisasi kabupaten/kota dapat meningkatkan hasil belajar Pendidikan Kewarganegaraan materi pemerintahan kabupaten/kota pada siswa kelas IV SD Negeri 010 Lubuk Keranji Kecamatan Bandar Petalangan Kabupaten Pelalawan.