bluuebunny/crossref_metadata_2025_split huggingface dataset:
Biological Control of Collar Rot on Passion Fruits Via Induction of Apoptosis in the Collar Rot Pathogen by Bacillus subtilis
Authors: Chen Yu-Hsuan , Lee Pei-Chun , Huang Tzu-Pi 
Published: October 2020

The seedlings and fresh fruits of passion fruits are of high value in local and global trade. Fusarium solani is a main disease-causing agent affecting passion fruits. The objectives of this study were to develop Bacillus-based biocontrol agents for the management of Fusarium diseases on passion fruits and to investigate their putative control mechanisms. Our studies indicated that B. subtilis YBC and 151B1 show antagonistic activity to F. solani PF7 from passion fruits and inhibited the conidial germination of strain PF7. The application of broth cultures from B. subtilis 151B1 and YBC in SYB medium reduced disease severity of Fusarium wilt on the leaves of passion fruits and enhanced the survival rates of passion fruit seedlings challenged with F. solani PF7. With regard to the putative mechanisms of disease control, the results indicated that treatments consisting of the respective culture filtrates from B. subtilis 151B1 and YBC broths caused aberrant conidial morphology and loss of cell membrane integrity. Additionally, the treatments caused reductions in mitochondrial membrane potential and interfered with the energy metabolism of F. solani PF7. The treatments also enhanced reactive oxygen species accumulation and resulted in the externalization of phosphatidylserine, chromatin condensation, and DNA fragmentation, suggesting their function in triggering apoptotic-like cell death. In conclusion, B. subtilis 151B1 and YBC are potential biocontrol agents for passion fruit disease caused by F. solani. Their control efficacy may result from the surfactins produced to trigger apoptotic-like cell death, reducing mitochondrial membrane potential and interfering with the energy metabolism of the pathogen. [Formula: see text] Copyright © 2021 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .
Rubella virus-associated chronic inflammation in primary immunodeficiency diseases
Authors: Perelygina Ludmila , Icenogle Joseph , Sullivan Kathleen E. 
Published: October 2020

Purpose of the review
The aim of this article is to summarize recent data on rubella virus (RuV) vaccine in chronic inflammation focusing on granulomas in individuals with primary immunodeficiencies (PIDs).

Recent findings
The live attenuated RuV vaccine has been recently associated with cutaneous and visceral granulomas in children with various PIDs. RuV vaccine strain can persist for decades subclinically in currently unknown body site(s) before emerging in granulomas. Histologically, RuV is predominately localized in M2 macrophages in the granuloma centers. Multiple mutations accumulate during persistence resulting in emergence of immunodeficiency-related vaccine-derived rubella viruses (iVDRVs) with altered immunological, replication, and persistence properties. Viral RNA was detected in granuloma biopsies and nasopharyngeal secretions and infectious virus were isolated from the granuloma lesions. The risk of iVDRV transmissibility to contacts needs to be evaluated. Several broad-spectrum antiviral drugs have been tested recently but did not provide significant clinical improvement. Hematopoietic stem cell transplantation remains the only reliable option for curing chronic RuV-associated granulomas in PIDs.

Summary
Persistence of vaccine-derived RuVs appears to be a crucial factor in a significant proportion of granulomatous disease in PIDs. RuV testing of granulomas in PID individuals might help with case management.
Association between Neuraxial Labor Analgesia and Neonatal Morbidity after Operative Vaginal Delivery
Authors: Butwick Alexander J. , Wong Cynthia A. , Lee Henry C. , Blumenfeld Yair J. , Guo Nan 
Published: October 2020

Background
Up to 84% of women who undergo operative vaginal delivery receive neuraxial analgesia. However, little is known about the association between neuraxial analgesia and neonatal morbidity in women who undergo operative vaginal delivery. The authors hypothesized that neuraxial analgesia is associated with a reduced risk of neonatal morbidity among women undergoing operative vaginal delivery.

Methods
Using United States birth certificate data, the study identified women with singleton pregnancies who underwent operative vaginal (forceps- or vacuum-assisted delivery) in 2017. The authors examined the relationships between neuraxial labor analgesia and neonatal morbidity, the latter defined by any of the following: 5-min Apgar score less than 7, immediate assisted ventilation, assisted ventilation greater than 6 h, neonatal intensive care unit admission, neonatal transfer to a different facility within 24 h of delivery, and neonatal seizure or serious neurologic dysfunction. The authors accounted for sociodemographic and obstetric factors as potential confounders in their analysis.

Results
The study cohort comprised 106,845 women who underwent operative vaginal delivery, of whom 92,518 (86.6%) received neuraxial analgesia. The proportion of neonates with morbidity was higher in the neuraxial analgesia group than the nonneuraxial group (10,409 of 92,518 [11.3%] vs. 1,271 of 14,327 [8.9%], respectively; P < 0.001). The unadjusted relative risk was 1.27 (95% CI, 1.20 to 1.34; P < 0.001); after accounting for confounders using a multivariable model, the adjusted relative risk was 1.19 (95% CI, 1.12 to 1.26; P < 0.001). In a post hoc analysis, after excluding neonatal intensive care unit admission and neonatal transfer from the composite outcome, the effect of neuraxial analgesia on neonatal morbidity was not statistically significant (adjusted relative risk, 1.07; 95% CI, 1.00 to 1.16; P = 0.054).

Conclusions
In this population-based cross-sectional study, a neonatal benefit of neuraxial analgesia for operative vaginal delivery was not observed. Confounding by indication may explain the observed association between neuraxial analgesia and neonatal morbidity, however this dataset was not designed to evaluate such considerations.

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Science of Learning Strategy Series: Article 1, Distributed Practice
Authors: Van Hoof Thomas J. , Sumeracki Megan A. , Madan Christopher R. 
Published: October 2020

Abstract:
Distributed practice is an evidence-based, learning-science strategy that is relevant to the planning and implementation of continuing professional development (CPD). Spacing-out study or practice over time allows the brain multiple opportunities to process new and complex information in an efficient way, thus increasing the likelihood of mastery and memory. Research from cognitive psychology and neuroscience provide the rationale for distributed practice, and examples of its implementation in health professions education have begun to appear in the literature. If used appropriately or extended creatively, some common CPD interventions can fully leverage distributed practice. Through increased understanding, CPD planners can benefit from distributed practice in efforts to improve educational activities, and CPD participants can benefit by making more informed educational choices.
Newer risk assessment strategies in hypertrophic cardiomyopathy
Authors: Chaudhry-Waterman Nadia , Cohen Mitchell I. 
Published: October 2020

Purpose of review
The present article serves to review current risk assessment guidelines for sudden cardiac death (SCD) in patients with hypertrophic cardiomyopathy (HCM) and to discuss how these guidelines can be applied to patients with childhood HCM. New diagnostic techniques that could lead to more accurate risk assessment tools are also discussed.

Recent findings
Current guidelines for risk assessment in childhood HCM are extrapolated from adult guidelines and lack background research to validate their use. Continuous variables, such as wall thickness, are converted to binary variables, which is particularly concerning in pediatric patients’ where weight gain and linear growth is likely to lead to more significant hemodynamic changes in shorter periods of time. Some studies have even shown that risk factors concerning in adults may actually be protective in pediatric patients. Additionally, large gaps still remain between genotype and phenotype expression in HCM.

Summary
A better understanding of the relationship between cause, phenotype, and outcomes is needed to truly be able to determine risk for SCD in childhood HCM. Larger studies, including newer technologies and quantitative models, similar to the European HCM Risk-SCD model, which allows for a quantitative risk diagnosis, are needed as well.
Effect of Ketamine on Rumination in Treatment-Resistant Depressive Patients
Authors: Vidal Sonia , Jermann Françoise , Aubry Jean-Michel , Richard-Lepouriel Hélène , Kosel Markus 
Published: October 2020

Abstract

Background
A rapid antidepressant effect of ketamine has repeatedly been documented in the literature, and identifying clinical features associated with a better response to this treatment is currently an essential question. Considering the relationship between rumination and depression and the need to identify potential predictors of response to ketamine, we analyzed the effect of a single injection of ketamine 0.5 mg/kg on rumination in treatment-resistant depressive (TRD) patients and explored whether baseline ruminative style and early improvements of rumination would predict a greater antidepressant effect of ketamine.

Methods
Ten TRD outpatients who participated in a 4-week open study on the antidepressant effect of ketamine also completed the Ruminative Response Scale the day before, the day after, and a week after ketamine administration.

Results
We found that in our patients, a single rapid 1-minute intravenous injection of ketamine 0.5 mg/kg was efficacious in reducing rumination, but neither severity of rumination at baseline nor early improvements of rumination after ketamine injection predicted antidepressant response.

Conclusions
Our preliminary data suggest that a single injection of ketamine 0.5 mg/kg can be efficacious in reducing rumination in TRD patients but rumination does not seem to be a useful clinical predictor of response to ketamine. Larger studies are necessary to confirm these results.
The past the present and the future of face transplantation
Authors: Siemionow Maria 
Published: October 2020

Purpose of review
Face transplantation represents vascularized composite allotransplantation (VCA) organ and became one of the most rewarding reconstructive options for severely disfigured patients. This review summarizes the past, current and future challenges of face transplantation, based on our experience and literature reports.

Recent findings
In 2005, first partial face transplantation was reported by French team. In 2008, we have performed the US first near-total face transplantation. Currently, more than 40 face transplant cases were reported worldwide. Based on the outcomes of our three patients and the literature reports, face transplantation improved aesthetics, function and the quality of life of face transplant patients. However, there are still many challenges encountered including the side effects of immunosuppressive protocols, the psychological and social problems as well as the financial challenges which need to be address in the near future to maintain face transplantation in the armamentarium of reconstructive surgery.

Summary
Currently, feasibility of face transplantation was confirmed; however, the life-long immunosuppressive protocols bearing serious side effects are still required to prevent face rejection. Thus, for the future of face and other VCA, novel approaches of cell-based therapies or engineered scaffolds should be developed to make face transplantation safer.
Donor-specific antibodies in kidney transplantation: the University of Wisconsin experience
Authors: Garg Neetika , Parajuli Sandesh , Mandelbrot Didier A. , Djamali Arjang 
Published: October 2020

Purpose of reviewAntibody-mediated rejection (ABMR) is the leading cause of allograft failure in kidney transplant recipients. This article provides a summary of the clinical research relating to donor-specific antibodies (DSA) and ABMR in kidney transplant recipients at the University of Wisconsin-Madison Transplant Center.Recent findingsOver 40% of the kidney transplant candidates on the UNOS waitlist are sensitized, and both preformed and de novo DSA are associated with increased risk of rejection and graft loss. We have developed graded induction-desensitization treatment and monitoring protocols based on the degree of immunologic risk. We have also implemented standard treatment and surveillance strategies for patients with ABMR. Additional important observations from our studies include high rates of ABMR in patients with positive C4d staining in postreperfusion biopsies and rise in DSA at 1 week after transplant, and increased risk of kidney allograft failure in patients with de novo DSA and ABMR, as well as in patients with HLA-DSA undetectable ABMR. We also found worse outcomes with de novo DSA following simultaneous pancreas--kidney and liver--kidney transplantation. Notably, favorable long-term graft outcomes were observed in patients with DSA who do not present the classic histopathological findings of ABMR.SummaryIn order to improve long-term outcomes for kidney transplant recipients, further research focusing on the pathogenic mechanisms elicited by HLA and non-HLA DSA, and novel therapies targeting these pathways is needed.
Early application of topical antibiotic powder in open-fracture wounds
Authors: Burbank Kimberly M. , Schauer Steven G. , De Lorenzo Robert A. , Wenke Joseph C. 
Published: October 2020

Abstract
Despite meticulous surgical care and systemic antibiotics, open fracture wounds have high rates of infection leading to increased morbidity. To reduce infection rates, orthopaedic surgeons may administer local antibiotics using various carriers that may be ineffective due to poor antibiotic release from carriers, subsequent surgery to remove nondegradable carriers, and mismatch between release kinetics and material degradation. Biofilms form rapidly as bacteria that are within the wound multiply quickly and transform from the antibiotic-susceptible planktonic phenotype to the antibiotic-tolerant biofilm phenotype. This tolerance to antibiotics can occur within hours. Currently, local antibiotics are placed in the wounds using a carrier such as polymethylmethacrylate beads; however, this occurs after surgical debridement that can be hours to even a day after initial injury allowing bacteria enough time to form a biofilm that makes the antibiotic containing polymethylmethacrylate beads less effective. In contrast, emerging practices in elective surgical procedures, such as spine fusion, place antibiotic powder (e.g. vancomycin) in the wound at the time of closure. This has been shown to be extremely effective, presumably because of the very small-time period between potential contamination and local antibiotic application. There is evidence that suggests that the ineffectiveness of local antibiotic use in open fractures is primarily due to the delay in application of local antibiotics from the time of injury and propose a concept of topical antibiotic powder application in the prehospital or emergency department setting.
Did Pre-exposure Prophylaxis Roll-Out Influence the Epidemic of Rectal Lymphogranuloma Venereum in Belgium? Results From the National Surveillance System
Authors: De Baetselier Irith , Tsoumanis Achilleas , Florence Eric , Van den Berghe Wim , Crucitti Tania , Van den Bossche Dorien , Kenyon Chris 
Published: October 2020

Background:
An increase of lymphogranuloma venereum (LGV) in HIV negative men who have sex with men is reported in several European countries including Belgium before the implementation of pre-exposure prophylaxis (PrEP).

Setting:
The epidemiological characteristics of the male rectal LGV epidemic in Belgium were explored before and after the introduction of PrEP.

Methods:
Segmented regression models were used to examine a change in trends before and after the introduction of PrEP in the male rectal LGV epidemic in Belgium and among men attending a large HIV/sexually transmitted infection clinic in Antwerp, Belgium.

Results:
Although an increase of 69% was noted in absolute numbers in 2019 compared with 2018 (140 vs 83 cases) in Belgium, models showed that the rate of increase did not change after the introduction of PrEP. More than half of the cases were found in HIV-negative men (56.2%) in 2019, but no difference in the magnitude of the trend was found after the introduction of PrEP. Nevertheless, the data reveal that a statistical significant increase of LGV prevalence was noted among non–HIV-positive men in an HIV/sexually transmitted infection clinic after the implementation of PrEP. Indeed, LGV prevalence in the Antwerp male PrEP cohort increased from 0.8% in 2017 to 2.4% in 2019.

Conclusions:
The trend of LGV increase did not accelerate after the introduction of PrEP. Continued surveillance in men who have sex with men irrespective of their HIV status is required for the management and control of the LGV epidemic.
How might controlled fusion fit into the emerging low-carbon energy system of the mid-twenty-first century?
Authors: Tynan G. R. , Abdulla A. 
Published: October 2020

We examine the characteristics that fusion-based generation technologies will need to have if they are to compete in the emerging low-carbon energy system of the mid-twenty-first century. It is likely that the majority of future electric energy demand will be provided by the lowest marginal cost energy technology—which in many regions will be stochastically varying renewable solar and wind electric generation coupled to systems that provide up to a few days of energy storage. Firm low-carbon or zero-carbon resources based on gas-fired turbines with carbon capture, advanced fission reactors, hydroelectric and perhaps engineered geothermal systems will then be used to provide the balance of load in a highly dynamic system operating in competitive markets governed by merit-order pricing mechanisms that select the lowest-cost supplies to meet demand. These firm sources will have overnight capital costs in the range of a few $/Watt, be capable of cycling down to a fraction of their maximum power output, operate profitably at low utilization fraction, and have a suitable unit size of order 100 MWe. If controlled fusion using either magnetic confinement or inertial confinement approaches is to have any chance of providing a material contribution to future electrical energy needs, it must demonstrate these key qualities and at the same time prove robust safety characteristics that avoid the perceived dread risk that plagues nuclear fission power, avoid the generation of long-lived radioactive waste and demonstrate highly reliable operations.This article is part of a discussion meeting issue ‘Prospects for high gain inertial fusion energy (part 1)’.
Crossed beam energy transfer between optically smoothed laser beams in inhomogeneous plasmas
Authors: Hüller Stefan , Raj Gaurav , Luo Mufei , Rozmus Wojciech , Pesme Denis 
Published: October 2020

Crossed beam energy transfer, CBET, in high-intensity laser–plasma interaction is investigated for the case of optically smoothed laser beams. In the two approaches to laser-driven inertial confinement fusion experiments, the direct-drive and the indirect-drive, CBET is of great importance because it governs the coupling of laser energy to the plasma. We use the two-dimensional wave-coupling code H
armony
to simulate the transfer between two laser beams with speckle structure that overlap in a plasma with an inhomogeneous flow profile. We compare the CBET dynamics for laser beams with spatial incoherence and with spatio-temporal incoherence; in particular we apply the smoothing techniques using random phase plates (RPPs) and smoothing by spectral dispersion (SSD), respectively. It is found that for laser beams (wavelength
λ
0
) with intensities (
I

L

) above
I

L

∼ 2 × 10
15
W cm
−2
(
λ
0
/0.35 µm)
−2
(
T

e

/keV), both the so-called plasma-induced smoothing as well as self-focusing in intense laser speckles induce temporal incoherence; the latter affects the CBET and the angular distribution of the light transmitted behind the zone of beam overlap. For RPP-smoothed incident beams, the resulting band width of the transmitted light can already be of the same order as the effective band width of the SSD available at major laser facilities. We examine the conditions when spatio-temporal smoothing techniques become efficient for CBET.

This article is part of a discussion meeting issue ‘Prospects for high gain inertial fusion energy (part 1)’.
Determinants of Time to Convalescence among COVID-19 Patients at Millennium COVID-19 Care Center in Ethiopia: A prospective cohort study
Authors: Leulseged Tigist W. , Hassen Ishmael S. , Maru Endalkachew H. , Zewde Wuletaw C. , Chamesew Nigat W. , Yegile Kalkidan T. , Bayisa Abdi B. , Jagema Tariku B. , Admasu Teketel T. , Edo Mesay G. , Gurara Eyosias K. , Hassen Meseret D. , Menyelshewa Etsegenet Y. , Abdi Firaol M. , Tefera Mahlet B. , Ali Siham S. 
Published: October 2020

ABSTRACTAimTo estimate time to recovery/convalescence and identify determinants among COVID-19 infected patients admitted to Millennium COVID-19 Care Center in Addis Ababa, Ethiopia.MethodsA prospective cohort study was conducted among a randomly selected sample of 360 COVID-19 patients who were on follow up from 2ndJune to 5thJuly 2020. Kaplan Meier plots, median survival times, and Log-rank test were used to describe the data and compare survival distribution between groups. Association between time to recovery/ convalescence and determinants was assessed using the Cox proportional hazard survival model, where hazard ratio, P-value, and 95% CI for hazard ratio were used for testing significance.ResultsThe mean age of the participants was 32.4 years (± 12.5 years). On admission, 86.9 % had mild COVID-19, 78.6% were asymptomatic and 11.4% of the patients had a history of pre-existing co-morbid illness. The Median time to recovery/ convalescence among the study population was 16 days. The log-rank test shows that having non-mild (moderate and severe) disease, having one or more symptoms at presentation, and presenting with respiratory and constitutional symptoms seems to extend the time needed to achieve recovery. The Final Cox regression result shows that the presence of symptom at presentation was found to be a significant factor that affects time to recovery/ convalescence, the rate of achieving recovery/ convalescence among symptomatic patients was 44% lower than patients who were asymptomatic at presentation (HR= 0.560, 95% CI= 0.322-0.975, p-value=0.040).ConclusionsPresence of symptom was found to be associated with delayed viral clearance. This implies symptomatic patients are more likely to be infectious because of the prolonged viral shedding in addition to the presence of a more concentrated virus in the upper respiratory tract that enhances the transmission. Therefore, attention should be given in the isolation and treatment practice of COVID-19 patients with regard to presence of symptom.Key MessagesThe study assessed the time to RT-PCR proven recovery (two consecutive negative viral shedding) and identified determinants that affected the time.Symptomatic infection is associated with delayed viral clearance.The finding of the study could be used to guide the isolation and treatment practice.