bluuebunny/crossref_metadata_2025_split huggingface dataset:
Contrasting responses of native and alien plant species to soil properties shed new light on the invasion of dune systems
Authors: Vitti Stefano , Pellegrini Elisa , Casolo Valentino , Trotta Giacomo , Boscutti Francesco 
Published: August 2020

Abstract

Aims
Among terrestrial ecosystems, coastal sandy dunes are particularly prone to alien plant invasion. Many studies related the invasion of dune habitats to anthropic causes, but less is known about the role of soil properties and plant traits in plant invasion. In this study, we tested the relationships between soil features and alien plant invasion in dune systems, focusing on the interplay between soil nutrients, soil salinity and plant functional traits.

Methods
Study sites were sandy barrier islands of the Marano and Grado lagoon (northern Adriatic Sea). One hundred plots (4 m × 4 m) were selected within 10 areas according to the main habitats occurring along the ecological gradient of dune system (foredune, backdune and saltmarsh). In each plot, we recorded all plant species occurrence and abundance and we collected a soil core. For each soil sample, soil texture, conductivity (as proxy of soil salinity), organic carbon and nitrogen content were analyzed and related to the species number and cover of native and alien plants. Variation of main reproductive and vegetative functional traits among habitats was also analyzed for both alien and native species.

Important Findings
Soil properties were strongly related to overall plant diversity, by differently affecting alien and native species pools. In backdune, the most invaded habitat, a high soil conductivity limited the number of alien species, whereas the content of soil organic carbon increased along with alien plant abundance, suggesting also the occurrence of potential feedback processes between plant invasion and soil. We found a significant convergence between native and alien plant functional trait spectra only in backdune habitat, where environmental conditions ameliorate and plant competition increases. Our findings suggest that in harsh conditions only native specialized plants can thrive while at intermediate conditions, soil properties gradient acts in synergy with plant traits to curb/facilitate alien plant richness.
The impacts of warming and nitrogen addition on competitive ability of native and invasive populations of Plantago virginica
Authors: Luo Xi , Zheng Yi , Xu Xiaohong , Xiao Rui , Guo Hui 
Published: August 2020

Abstract

Aims
Global change factors (e.g. warming and nitrogen deposition) may influence biological invasions, but how these factors may influence the performance of invasive species and further mediate the interactions with native competitors remain still unknown.

Methods
Here, we conducted a 5-month greenhouse experiment to examine the effects of warming (using open-top chambers, +0.62°C) and N addition (adding NH4NO3 at a rate of 4.2 g m−2) on the performance of the native and invasive populations of an invasive species Plantago virginica in competition with a native Plantago asiatica.

Important Findings
Under warming treatment and its interaction with nitrogen addition treatment (W × N), invasive and native populations of P. virginica had different biomass allocation strategies to compete with native competitor P. asiatica. Native population of P. virginica (PV-Na) increased more below-ground biomass, whereas those from the invasive population (PV-In) increased more above-ground biomass. We also found that invasive species P. virginica had stronger responses to warming and N addition than the native species P. asiatica. The competitive ability of the invasive plants was significantly reduced by warming which indicated that the invasive plant were much stronger sensitivity to elevated temperature than native plant. Similarly, N addition and W × N reduced the competitive response of PV-In in below-ground biomass, but increased the competitive response of PV-Na in above-ground and total biomass when they grew with the P. asiatica. The results show that P. virginica have occurred differential biomass allocation strategies during its invasions and invasive population exhibit flexible competition ability to adapt to environmental changes (especially warming). These findings may potentially help to predict plant invasions and make management strategies in a world with changing climate.
Spectrophotometric characterization of the Philae landing site and surroundings with the Rosetta/OSIRIS cameras
Authors: Hoang Hong Van , Fornasier S , Quirico E , Hasselmann P H , Barucci M A , Sierks H , Tubiana C , Güttler C 
Published: August 2020

ABSTRACT
We investigate Abydos, the final landing site of the Philae lander after its eventful landing from the Rosetta spacecraft on comet 67P/Churyumov–Gerasimenko on 2014 November 12. Over 1000 OSIRIS-level 3B images were analysed, which cover the 2014 August–2016 September timeframe, with spatial resolution ranging from 7.6 m pixel−1 to approximately 0.06 m pixel−1. We found that the Abydos site is as dark as the global 67P nucleus and spectrally red, with an average albedo of 6.5 per cent at 649 nm and a spectral slope value of about 17 per cent/(100 nm) at 50° phase angle. Similar to the whole nucleus, the Abydos site also shows phase reddening but with lower coefficients than other regions of the comet, which may imply a thinner cover of microscopically rough regolith compared to other areas. Seasonal variations, as already noticed for the whole nucleus, were also observed. We identified some potential morphological changes near the landing site implying a total mass-loss of (4.7–7.0) × 105 kg. Small spots ranging from 0.1 to 27 m2 were observed close to Abydos before and after perihelion. Their estimated water ice abundance reaches 30–40 per cent locally, indicating fresh exposures of volatiles. Their lifetime ranges from a few hours up to three months for two pre-perihelion spots. The Abydos surroundings showed a low level of cometary activity compared to other regions of the nucleus. Only a few jets are reported originating nearby Abydos, including a bright outburst that lasted for about 1 h.
The hierarchical fragmentation of filaments and the role of sub-filaments
Authors: Clarke S D , Williams G M , Walch S 
Published: August 2020

ABSTRACT
Recent observations have revealed the presence of small fibres or sub-filaments within larger filaments. We present a numerical fragmentation study of fibrous filaments investigating the link between cores and sub-filaments using hydrodynamical simulations performed with the moving-mesh code arepo. Our study suggests that cores form in two environments: (i) as isolated cores, or small chains of cores, on a single sub-filament, or (ii) as an ensemble of cores located at the junction of sub-filaments. We term these isolated and hub cores, respectively. We show that these core populations are statistically different from each other. Hub cores have a greater mean mass than isolated cores, and the mass distribution of hub cores is significantly wider than isolated cores. This fragmentation is reminiscent of parsec-scale hub-filament systems, showing that the combination of turbulence and gravity leads to similar fragmentation signatures on multiple scales, even within filaments. Moreover, the fact that fragmentation proceeds through sub-filaments suggests that there exists no characteristic fragmentation length-scale between cores. This is in opposition to earlier theoretical works studying fibre-less filaments which suggest a strong tendency towards the formation of quasi-periodically spaced cores, but in better agreement with observations. We also show tentative signs that global collapse of filaments preferentially form cores at both filament ends, which are more massive and dense than other cores.
The molecular mechanisms that underlie fragile X-associated premature ovarian insufficiency: is it RNA or protein based?
Authors: Rosario Roseanne , Anderson Richard 
Published: August 2020

Abstract
The FMR1 gene contains a polymorphic CGG trinucleotide sequence within its 5′ untranslated region. More than 200 CGG repeats (termed a full mutation) underlie the severe neurodevelopmental condition fragile X syndrome, while repeat lengths that range between 55 and 200 (termed a premutation) result in the conditions fragile X-associated tremor/ataxia syndrome and fragile X-associated premature ovarian insufficiency (FXPOI). Premutations in FMR1 are the most common monogenic cause of premature ovarian insufficiency and are routinely tested for clinically; however, the mechanisms that contribute to the pathology are still largely unclear. As studies in this field move towards unravelling the molecular mechanisms involved in FXPOI aetiology, we review the evidence surrounding the two main theories which describe an RNA toxic gain-of-function mechanism, resulting in the loss of function of RNA-binding proteins, or a protein-based mechanism, where repeat-associated non-AUG translation leads to the formation of an abnormal polyglycine containing protein, called FMRpolyG.
Evidence for stage-based larval vulnerability and resilience to acidification in Crassostrea virginica
Authors: Miller A Whitman , Reynolds Amanda , Minton Mark S , Smith Rachel 
Published: August 2020

ABSTRACT
Using image analysis of scanning electron micrographs (SEMs), we compared differences in growth of D-stage veligers [i.e. prodissoconch I and II (PI and PII) larvae] of eastern oysters Crassostrea virginica grown in mesohaline water under high- and low-CO2 conditions. We found SEMs to reveal no evidence of dissolution or shell structure deformity for larval shells in either of the CO2 treatments but detected prominent growth lines in the PII regions of larval shells. The number of growth lines closely approximated the duration of the experiment, suggesting that growth lines are generated daily. Mean growth line interval widths were 20% greater for larval shells cultured in low- vs high-CO2 conditions. Crassostrea virginica veliger larvae were shown to tolerate high CO2 levels and aragonite saturation states (Ωarag) &lt; 1.0, but larval growth was slowed substantially under these conditions. Differences in growth line interval width translate into substantial changes in shell area and account for previously observed differences in total shell area between the treatments, as determined by light microscopy and image analysis. Other studies have documented high mortality and malformation of D-stage larvae in bivalves when pre-veliger life stages (i.e. eggs, gastrula and trochophores) were exposed to elevated CO2. Our experiments revealed statistical differences in rates of larval survival, settlement and subsequent early-stage spat mortality for veligers reared in high- and low-CO2 conditions. Although each of these rates was measurably affected by high CO2, the magnitude of these differences was small (range across categories = 0.7–6.3%) suggesting that the impacts may not be catastrophic, as implied by several previous studies. We believe the apparent disparity among experimental results may be best explained by differential vulnerability of pre-veliger stage larvae and veligers, whereby PI and PII larvae have greater physiological capacity to withstand environmental conditions that may be thermodynamically unfavourable to calcification (i.e. Ωarag &lt; 1.0).
Evidence for Multi-stage Melt Transport in the Lower Ocean Crust: the Atlantis Bank Gabbroic Massif (IODP Hole U1473A, SW Indian Ridge)
Authors: Zhang Wei-Qi , Liu Chuan-Zhou , Dick Henry J B 
Published: August 2020

AbstractThe architecture of lower oceanic crust at slow- and ultraslow-spreading ridge is diverse, yet the mechanisms that produce this diversity are not well understood. Particularly, the 660-km2 gabbroic massif at Atlantis Bank (Southwest Indian Ridge) exhibits significant compositional zonation, representing a high magma supply end member for accretion of the lower ocean crust at slow and ultraslow-spreading ridges. We present the petrographic and geochemical data of olivine gabbros from the 809-metre IODP Hole U1473A at Atlantis Bank gabbroic massif. Structurally, the upper portion of U1473A consists of a ∼600-metre shear zone; below this, the hole is relatively undeformed, with several minor shear zones. Olivine gabbros away from the shear zones have mineral trace element compositions indicative of high-temperature reaction with an oxide-undersaturated melt. By contrast, olivine gabbros within shear zones display petrographic and chemical features indicative of reaction with a relatively low-temperature, oxide-saturated melt. These features indicate an early stage of primitive to moderately evolved melt migration, followed by deformation-driven transport of highly evolved Fe–Ti-rich melts to high levels in this gabbroic massif. The close relationship between shear zones and the reaction with oxide-saturated melts suggests that syn-magmatic shear zones provide a conduit for late-stage, Fe–Ti-rich melt transport through Atlantis Bank lower crust. This process is critical to generate the compositional zonation observed. Thus, the degree of syn-magmatic deformation, which is fundamentally related to magma supply, plays a dominant role in developing the diversity of lower ocean crust observed at slow- and ultraslow-spreading ridges.
Neutrophil subpopulations and their activation potential in patients with antiphospholipid syndrome and healthy individuals
Authors: Mauracher Lisa-Marie , Krall Moritz , Roiß Johanna , Hell Lena , Koder Silvia , Hofbauer Thomas M , Gebhart Johanna , Hayden Hubert , Brostjan Christine , Ay Cihan , Pabinger Ingrid 
Published: August 2020

Abstract

Objectives
Patients with APS are at increased risk of thromboembolism. Neutrophils have been shown to play a role in inducing thrombosis. We aimed to investigate differences in neutrophil subpopulations, their potential of activation and neutrophil extracellular trap (NET) formation comparing high and low-density neutrophils (HDNs/LDNs) as well as subpopulations in patients with APS and controls to gain deeper insight into their potential role in thrombotic manifestations in patients with APS.

Methods
HDNs and LDNs of 20 patients with APS and 20 healthy donors were isolated by density gradient centrifugation and stimulated. Neutrophil subpopulations, their activation and NET release were assessed by flow cytometry.

Results
LDNs of both groups showed higher baseline activation, lower response to stimulation (regulation of activation markers CD11b/CD66b), but higher NET formation compared with HDNs. In patients with APS, the absolute number of LDNs was higher compared with controls. HDNs of APS patients showed higher spontaneous activation [%CD11b high: median (interquartile range): 2.78% (0.58–10.24) vs 0.56% (0.19–1.37)] and response to stimulation with ionomycin compared with HDNs of healthy donors [%CD11b high: 98.20 (61.08–99.13) vs 35.50% (13.50–93.85)], whereas no difference was found in LDNs. NET formation was increased in patients’ HDNs upon stimulation.

Conclusion
HDNs and LDNs act differently, unstimulated and upon various stimulations in both healthy controls and APS patients. Differences in HDNs and LDNs between patients with APS and healthy controls indicate that neutrophils may enhance the risk of thrombosis in these patients and could thus be a target for prevention of thrombosis in APS.
Transfusion-transmissible dengue infections
Authors: Perera Lakshika , De Zoysa Nandrani , Jayarajah Umesh , Senanayake Nilanthi , De Zoysa Ishan , Seneviratne Suranjith L 
Published: August 2020

Abstract
Dengue is an important global health problem and is endemic in many developing and developed countries. Transmission of dengue may occur in several ways and information on transfusion-transmitted dengue is limited. We conducted a literature search on transfusion-related dengue using the PubMed, Scopus, Embase and Google Scholar databases and have summarized the findings. A number of apparently healthy blood donors have been found to be infected with the dengue virus (DENV) and thus may transmit the virus onto recipients of this blood. It is not possible to identify and exclude such donors at the donor selection stage and thus reliable screening tests should be available in highly endemic areas to ensure a safe blood supply.
The impact of heart rate on pulse wave velocity: an in-silico evaluation
Authors: Bikia Vasiliki , Stergiopulos Nikolaos , Rovas Georgios , Pagoulatou Stamatia , Papaioannou Theodore G. 
Published: August 2020

Background:
Clinical and experimental evidence regarding the influence of heart rate (HR) on arterial stiffness and its surrogate marker carotid-to-femoral pulse wave velocity (cf-PWV) is conflicting. We aimed to evaluate the effect of HR on cf-PWV measurement under controlled haemodynamic conditions and especially with respect to blood pressure (BP) that is a strong determinant of arterial stiffness.

Method:
Fifty-nine simulated cases were created using a previously validated in-silico model. For each case, cf-PWV was measured at five HR values, 60, 70, 80, 90, 100 bpm. With increasing HR, we assessed cf-PWV under two scenarios: with BP free to vary in response to HR increase, and with aortic DBP (aoDBP) fixed to its baseline value at 60 bpm, by modifying total peripheral resistance accordingly. Further, we quantified the importance of arterial compliance (C) on cf-PWV changes caused by increasing HR.

Results:
When BP was left free to vary with HR, a significant HR-effect on cf-PWV (0.66 ± 0.24 m/s per 10 bpm, P < 0.001) was observed. This effect was reduced to 0.21 ± 0.14 m/s per 10 bpm (P = 0.048) when aoDBP was maintained fixed with increasing HR. The HR-effect on the BP-corrected cf-PWV was higher in the case of low C = 0.8 ± 0.3 ml/mmHg (0.26 ± 0.15 m/s per 10 bpm, P = 0.014) than the case of higher C = 1.7 ± 0.5 ml/mmHg (0.16 ± 0.07 m/s per 10 bpm, P = 0.045).

Conclusion:
Our findings demonstrated that relatively small HR changes may only slightly affect the cf-PWV. Nevertheless, in cases wherein HR might vary at a greater extent, a more clinically significant impact on cf-PWV should be considered.
Combined effect of high depressive symptom burden and hypertension on new-onset stroke: evidence from a nationwide prospective cohort study
Authors: Liu Siyuan , Qiao Yanan , Zhang Yuxia , Wu Ying , Ke Chaofu , Shen Yueping 
Published: August 2020

Objective:
The aim of this study was to investigate whether the combination of high depressive symptom burden and hypertension increased the risk of stroke among the middle-aged and elderly Chinese using a nationwide prospective study.

Methods:
Data from the China Health and Retirement Longitudinal Study (CHARLS) during 2011–2015 were used. A total of 12 604 Chinese participants aged 45 years and older were included for final analysis. Multivariate Cox proportional hazards regression model was used to explore the associations between high depressive symptom burden, hypertension and new-onset stroke.

Results:
There were 244 stroke events occurred during a 4-year follow-up. Compared with those without high depressive symptom burden and hypertension, the adjusted hazard ratios (95% confidence intervals) were 1.96 (1.13–3.42) for individuals with high depressive symptom burden alone, 2.84 (1.77–4.57) for individuals with hypertension alone and 4.38 (2.66–7.20) for individuals with comorbid high depressive symptom burden and hypertension, respectively. In the subgroup analyses, people with the coexistence of high depressive symptom burden and hypertension had the highest risk of new-onset stroke in all subgroups.

Conclusion:
Our results suggest a combined effect of high depressive symptom burden and hypertension on stroke risk among the middle-aged and elderly Chinese.
Cisapride Use in Pediatric Patients With Intestinal Failure and Its Impact on Progression of Enteral Nutrition
Authors: Martinez Andrea , Belza Christina , Betts Zachary , de Silva Nicole , Avitzur Yaron , Wales Paul W. 
Published: August 2020

ABSTRACTObjective:Gastrointestinal dysmotility is common in patients with pediatric intestinal failure (PIF), leading to delays in advancement of enteral nutrition (EN). Few studies have been published regarding the safety and efficacy of cisapride for improvement of enteral tolerance and ability to wean parenteral nutrition. Our objective was to describe a single center experience on the use of cisapride in patients with PIF.Methods:Retrospective chart review of patients was performed. Demographic, intestinal anatomy, and outcome data were collected. Percentage of EN before initiation of cisapride, progression of EN at 3 and 6 months, and ability to wean parenteral nutrition after initiation of cisapride were calculated.Results:Prokinetics were used in 61 of 106 patients (56.6%); 29 of 60 patients (48.3%) failed to progress EN on other prokinetics and started on cisapride. Before cisapride the progress of EN plateaued for a mean of 42.3 (standard deviation [SD] 60.2) days. The rate of feed progression was 0.14% (SD 0.19)/day pre‐cisapride and 0.69%/day (SD 0.31) after cisapride initiation (P < 0.001). Percentage of EN improved significantly from baseline to 3 months postinitiation (23.9% vs 79.4%, respectively; P < 0.001). Electrocardiogram was performed on initiation of cisapride and after every dose change. Medication was discontinued in 2 of 29 (6.8%).Conclusion:This retrospective study suggests that cisapride may be beneficial in PIF patients who fail to progress EN on first line prokinetics. The most significant period of improvement occurs within 3 months of cisapride initiation. Cardiac side effects in our cohort were lower than previously reported; however, cardiac monitoring is still recommended.
Use of Anthropometry Versus Ultrasound for the Assessment of Body Fat and Comorbidities in Children With Obesity
Authors: El‐Koofy Nehal , Soliman Hend , Elbarbary Mennat‐Allah , Garhy Al Shimaa El , Sheba Maha , Fouad Hanan 
Published: August 2020

ABSTRACTObjectives:We aimed to examine the association between abdominal fat measured by ultrasound and anthropometric indices in children with obesity, and those with normal weight. We also examined the association between anthropometry and fat measures in the prediction of comorbidities in children with obesity.Methods:Forty children with body mass index of >95th percentile were included as cases, and a comparable group of 32 healthy average‐weight peers were included as controls in this study. All children underwent clinical assessment, anthropometric measures, and evaluation of abdominal subcutaneous fat (SCF) and visceral fat by ultrasound. Fasting blood sugar, serum transaminases, and lipid profile of all the included children were also evaluated.Results:Children with obesity had a mean age of 8.7 ± 2.9 years (range 3–13). The SCF and intraperitoneal fat (IPF) values correlated well with each other and with anthropometric measurements in children with obesity. Among all the included cases, 90% were metabolically unhealthy, 70% had hypertension, 52.5% had dyslipidemia, and 22.5% had echogenic liver. Anthropometric measures, abdominal SCF and IPF were higher in children with complications. SCF was observed as a good predictor for hepatic echogenicity among the measured ultrasound parameters (P: 0.03, odds ratio 4.6). The best cutoff value for SCF in cases with hepatic echogenicity was 23.2 mm with an overall accuracy of 80%.Conclusions:In children with obesity, abdominal SCF and IPF correlated well with anthropometric measures and were higher in children with comorbidities. This finding, however, did not predict comorbidities apart from those with echogenic liver.