bluuebunny/crossref_metadata_2025_split huggingface dataset:
Glucocorticoid-Induced Leucine Zipper as a Druggable Target in Inflammatory Bowel Diseases
Authors: Ronchetti Simona , Gentili Marco , Ricci Erika , Migliorati Graziella , Riccardi Carlo 
Published: January 2020

Abstract
Inflammatory bowel diseases (IBDs) are chronic inflammatory disorders with a complex pathogenesis, affecting people of all ages. They are characterized by alternating phases of clinical relapse and remission, depending on the fine balance between immune cells and the gut microbiota. The cross talk between cells of the immune system and the gut microbiota can result in either tolerance or inflammation, according to multifactorial triggers, ranging from environmental factors to genetic susceptibility. Glucocorticoid (GC) administration remains the first-line treatment for IBDs, although long-term use is limited by development of serious adverse effects. Recently, new alternative pharmacological therapies have been developed, although these are not always effective in IBD patients. There is a constant demand for effective new drug targets to guarantee total remission and improve the quality of life for IBD patients. The glucocorticoid-induced leucine zipper (GILZ) has been implicated as a promising candidate for this purpose, in view of its powerful anti-inflammatory effects that mimic those of GCs while avoiding their unwanted adverse reactions. Here we present and discuss the latest findings about the involvement of GILZ in IBDs.
Performance estimation of membrane dehumidification based on heat exchanger analogy approaches using ε-NTU model
Authors: Lee Gilbong , Roh Chul Woo , Choi Bong Soo , Wang Eunseok , Ra Ho-Sang , Cho Junhyun , Baik Young-Jin , Lee Young-Soo , Shin Hyungki , Lee Beomjoon 
Published: January 2020

Abstract
Reports by the US Department of Energy in 2014 evaluated membrane heat pump technology as one of the most promising alternatives to conventional vapour compression methods. Vapour compression methods maintain an evaporator temperature lower than the dew point to deal with the latent heat load. In membrane heat pump systems, only the water vapour is transferred and there is no phase change. The migration is caused by the difference in vapour pressure before and after the membrane. A vacuum pump or blower is used to create the pressure difference. However, there is no methodology for predicting dehumidification performance of membranes when used as part of a cooling system. In this study, using the assumption that there is a similarity between heat transfer and moisture pervaporation, the performance indices of the membrane are derived using a well-known heat exchanger method, the ε-NTU models. Performance estimations are calculated for two representative system layouts: bypass and vacuum. Simple relations between design parameters are suggested, giving design guidelines for researchers.
Phosphocholine Antagonizes Listeriolysin O-Induced Host Cell Responses of Listeria monocytogenes
Authors: La Pietra Luigi , Hudel Martina , Pillich Helena , Abu Mraheil Mobarak , Berisha Besim , Aden Saša , Hodnik Vesna , Lochnit Günter , Rafiq Amir , Perniss Alexander , Anderluh Gregor , Chakraborty Trinad 
Published: January 2020

AbstractBackgroundBacterial toxins disrupt plasma membrane integrity with multitudinous effects on host cells. The secreted pore-forming toxin listeriolysin O (LLO) of the intracellular pathogen Listeria monocytogenes promotes egress of the bacteria from vacuolar compartments into the host cytosol often without overt destruction of the infected cell. Intracellular LLO activity is tightly controlled by host factors including compartmental pH, redox, proteolytic, and proteostatic factors, and inhibited by cholesterol.MethodsCombining infection studies of L. monocytogenes wild type and isogenic mutants together with biochemical studies with purified phospholipases, we investigate the effect of their enzymatic activities on LLO.ResultsHere, we show that phosphocholine (ChoP), a reaction product of the phosphatidylcholine-specific phospholipase C (PC-PLC) of L. monocytogenes, is a potent inhibitor of intra- and extracellular LLO activities. Binding of ChoP to LLO is redox-independent and leads to the inhibition of LLO-dependent induction of calcium flux, mitochondrial damage, and apoptosis. ChoP also inhibits the hemolytic activities of the related cholesterol-dependent cytolysins (CDC), pneumolysin and streptolysin.ConclusionsOur study uncovers a strategy used by L. monocytogenes to modulate cytotoxic LLO activity through the enzymatic activity of its PC-PLC. This mechanism appears to be widespread and also used by other CDC pore-forming toxin-producing bacteria.
Feasibility and Safety of Outpatient Parenteral Antimicrobial Therapy in Conjunction With Addiction Treatment for People Who Inject Drugs
Authors: Price Christin N , Solomon Daniel A , Johnson Jennifer A , Montgomery Mary W , Martin Bianca , Suzuki Joji 
Published: January 2020

Abstract

Background
Research is limited on combining outpatient parenteral antimicrobial therapy (OPAT) with addiction treatment for people who inject drugs (PWID) with serious infections.

Methods
This is a retrospective study of PWID (n = 68) requiring intravenous antibiotics evaluated for suitability for our OPAT program with concurrent addiction treatment.

Results
Most common infections were bacteremia and/or endocarditis (73.5%), bone and/or joint infections (32.4%), and epidural abscess (22.1%). Of the 20 patients (29.4%) who qualified, 100.0% completed the course of antibiotics, 30.0% experienced a 30-day readmission, and 15.0% relapsed. No overdoses, deaths, or peripherally inserted central catheter-line complications were reported.

Conclusions
Outpatient parenteral antimicrobial therapy with addiction treatment may be feasible and safe for PWID with serious infections.
Fly-on-a-Chip: Microfluidics for Drosophila melanogaster Studies
Authors: Zabihihesari Alireza , Hilliker Arthur J , Rezai Pouya 
Published: January 2020

Abstract
The fruit fly or Drosophila melanogaster has been used as a promising model organism in genetics, developmental and behavioral studies as well as in the fields of neuroscience, pharmacology, and toxicology. Not only all the developmental stages of Drosophila, including embryonic, larval, and adulthood stages, have been used in experimental in vivo biology, but also the organs, tissues, and cells extracted from this model have found applications in in vitro assays. However, the manual manipulation, cellular investigation and behavioral phenotyping techniques utilized in conventional Drosophila-based in vivo and in vitro assays are mostly time-consuming, labor-intensive, and low in throughput. Moreover, stimulation of the organism with external biological, chemical, or physical signals requires precision in signal delivery, while quantification of neural and behavioral phenotypes necessitates optical and physical accessibility to Drosophila. Recently, microfluidic and lab-on-a-chip devices have emerged as powerful tools to overcome these challenges. This review paper demonstrates the role of microfluidic technology in Drosophila studies with a focus on both in vivo and in vitro investigations. The reviewed microfluidic devices are categorized based on their applications to various stages of Drosophila development. We have emphasized technologies that were utilized for tissue- and behavior-based investigations. Furthermore, the challenges and future directions in Drosophila-on-a-chip research, and its integration with other advanced technologies, will be discussed.
Physiology and pathology of T-cell aging
Authors: Minato Nagahiro , Hattori Masakazu , Hamazaki Yoko 
Published: January 2020

Abstract
Acquired immune function shows recognizable changes over time with organismal aging. These changes include T-cell dysfunction, which may underlie diminished resistance to infection and possibly various chronic age-associated diseases in the elderly. T-cell dysfunction may occur at distinct stages, from naive cells to the end stages of differentiation during immune responses. The thymus, which generates naive T cells, shows unusually early involution resulting in progressive reduction of T-cell output after adolescence, but peripheral T-cell numbers are maintained through antigen-independent homeostatic proliferation of naive T cells driven by the major histocompatibility complex associated with self-peptides and homeostatic cytokines, retaining the diverse repertoire. However, extensive homeostatic proliferation may lead to the emergence of dysfunctional CD4+ T cells with features resembling senescent cells, termed senescence-associated T (SA-T) cells, which increase and accumulate with age. In situations such as chronic viral infection, T-cell dysfunction may also develop via persistent antigen stimulation, termed exhaustion, preventing possible immunopathology due to excessive immune responses. Exhausted T cells are developed through the effects of checkpoint receptors such as PD-1 and may be reversed with the receptor blockade. Of note, although defective in their regular T-cell antigen-receptor-mediated proliferation, SA-T cells secrete abundant pro-inflammatory factors such as osteopontin, reminiscent of an SA-secretory phenotype. A series of experiments in mouse models indicated that SA-T cells are involved in systemic autoimmunity as well as chronic tissue inflammation following tissue stresses. In this review, we discuss the physiological aspects of T-cell dysfunction associated with aging and its potential pathological involvement in age-associated diseases and possibly cancer.
Dietary soy isoflavones reduce pathogen-related mortality in growing pigs under porcine reproductive and respiratory syndrome viral challenge
Authors: Smith Brooke N , Oelschlager Maci L , Abdul Rasheed Muhammed Shameer , Dilger Ryan N 
Published: January 2020

Abstract
Porcine reproductive and respiratory syndrome virus (PRRSV) is an economically important disease, and ingestion of soy isoflavones (ISF) may benefit PRRSV-infected pigs due to demonstrated anti-inflammatory and antiviral properties. The objective of this experiment was to recreate immunological effects previously observed in young pigs infected with PRRSV receiving ISF and determine how those effects influence growth performance during the entire growth period from weaning to market. In total, 96 weaned barrows were group housed in a biosafety level-2 containment facility and allotted to 1 of 3 experimental treatments that were maintained throughout the study: noninfected pigs received an ISF-devoid control diet (NEG, n = 24), and infected pigs received either the control diet (POS, n = 36) or that supplemented with total ISF in excess of 1,600 mg/kg (ISF, n = 36). Following a 7-d adaptation, weanling pigs were inoculated intranasally with either a sham-control (PBS) or live PRRSV (1 × 105 TCID50/mL, strain NADC20). After inoculation, individual blood samples (n = 8 to 12/treatment) were routinely collected to monitor viral clearance and hematological parameters, including serum neutralizing anti-PRRSV antibody production. Pen-based oral fluids were used to monitor PRRSV clearance at later growth stages. A 1- or 2-way ANOVA was performed to compare experimental treatments depending on whether the outcome was repeatedly measured. In general, PRRSV infection decreased performance during early growth phases, resulting in 5.4% lower final BW for POS vs. NEG pigs (P &lt; 0.05). Dietary ISF elicited inconsistent effects on growth performance, increased (P &lt; 0.05) neutrophil cell counts and the relative proportion of memory T-cells, and decreased (P &lt; 0.05) the time to full PRRSV clearance from oral fluids. Dietary ISF also elicited earlier, more robust anti-PRRSV neutralizing antibody production when compared with POS pigs. Additionally, and most notably, POS pigs experienced ~50% greater infection-related mortality rate vs. ISF pigs (P &lt; 0.05), which may have significant economic implications for producers. Overall, dietary ISF ingestion supported immune responses and reduced mortality in PRRSV-infected pigs when fed to growing pigs though the biological mechanism of these effects remains unclear.
Investigation of Ayahuasca β-Carboline Alkaloids and Tryptamine in Sweat Samples from Religious Community Participants by GC-MS
Authors: Tavares Lidiane , Monedeiro Fernanda , Bordin Dayanne Mozaner , De Martinis Bruno Spinosa 
Published: January 2020

AbstractAyahuasca, a hallucinogenic beverage used in religious rituals in South America, has become a global phenomenon. Its main active components are the β-carbolines alkaloids, harmine (HRM) and harmaline (HRL), as well as the potent hallucinogen N,N-dimethyltryptamine (DMT). Despite its rising consumption, information regarding possible clinical applications and toxicological effects of ayahuasca is still limited. This study presents the first investigation of the use of sweat for the determination of DMT, HRM and HRL in ayahuasca users during a religious ritual. Sweat is an alternative matrix with advantages over many conventional biological samples, mainly because the collection procedure is non-invasive, easy and simple and samples can be collected without disturbing the religious ritual. In the study, solid-phase extraction was performed under basic conditions. Linearity was observed ranging from 20 to 1500 ng/patch with coefficients of determination (R2) higher than 0.99 for all analytes. The results indicated high selectivity for all investigated analytes, with extraction efficiency exceeding 70%, accuracy ranging from 87.5 to 102.4%, intra-assay precision of 1.85–9.44% and inter-assay precision between 3.34 and 9.85%. The limits of detection were 15 ng/patch for HRM and HRL and 10 ng/patch for DMT. The sweat proved to be a viable option to monitor ayahuasca use.
Comparative Survival of Asian and White Metastatic Castration-Resistant Prostate Cancer Men Treated With Docetaxel
Authors: Halabi Susan , Dutta Sandipan , Tangen Catherine M , Rosenthal Mark , Petrylak Daniel P , Thompson Ian M , Chi Kim N , De Bono Johann S , Araujo John C , Logothetis Christopher , Eisenberger Mario A , Quinn David I , Fizazi Karim , Morris Michael J , Higano Celestia S , Tannock Ian F , Small Eric J , Kelly William Kevin 
Published: January 2020

AbstractThere are few data regarding disparities in overall survival (OS) between Asian and white men with metastatic castration-resistant prostate cancer (mCRPC). We compared OS of Asian and white mCRPC men treated in phase III clinical trials with docetaxel and prednisone (DP) or a DP-containing regimen. Individual participant data from 8820 men with mCRPC randomly assigned on nine phase III trials to receive DP or a DP-containing regimen were combined. Men enrolled in these trials had a diagnosis of prostate adenocarcinoma. The median overall survival was 18.8 months (95% confidence interval [CI] = 17.4 to 22.1 months) and 21.2 months (95% CI = 20.8 to 21.7 months) for Asian and white men, respectively. The pooled hazard ratio for death for Asian men compared with white men, adjusted for baseline prognostic factors, was 0.95 (95% CI = 0.84 to 1.09), indicating that Asian men were not at increased risk of death. This large analysis showed that Asian men did not have shorter OS duration than white men treated with docetaxel.
Refugee-Run Grassroots Organizations: Responsive Assistance beyond the Constraints of US Resettlement Policy
Authors: Gonzalez Benson Odessa 
Published: January 2020

Abstract
Refugee-resettlement policy as constitutive of neoliberal governance has been critically examined as delimiting service provision by state-contracted refugee-serving agencies. What remains unexamined is how state-detached refugee-run organizations at the grassroots fit into the privatized, marketized and technocratic modalities of such governance. This study examines Refugee Community Organizations (RCOs) in the US and their scope of services in relation to publicly funded resettlement services, drawing on focus groups and 40 interviews with RCO leaders of Bhutanese communities in 35 US cities. Findings illustrate RCOs with a wide scope of services, in terms of eligibility, time limits, proximity and modality. Who and when: RCOs target those neglected by work-oriented policies and provide assistance well beyond policy time limits. Where and how: RCOs are closer to communities in terms of both geographical and sociocultural proximity. Issues of equity and social justice are thus raised, as RCOs aim to assume important functions of the state and pursue the mandates of federal policy, without adequate resources and legitimacy.
Endomembrane architecture and dynamics during secretion of the extracellular matrix of the unicellular charophyte, Penium margaritaceum
Authors: Domozych David S , Sun Li , Palacio-Lopez Kattia , Reed Reagan , Jeon Susan , Li Mingjia , Jiao Chen , Sørensen Iben , Fei Zhangjun , Rose Jocelyn K C 
Published: January 2020

AbstractThe extracellular matrix (ECM) of many charophytes, the assemblage of green algae that are the sister group to land plants, is complex, produced in large amounts, and has multiple essential functions. An extensive secretory apparatus and endomembrane system are presumably needed to synthesize and secrete the ECM, but structural details of such a system have not been fully characterized. Penium margaritaceum is a valuable unicellular model charophyte for studying secretion dynamics. We report that Penium has a highly organized endomembrane system, consisting of 150–200 non-mobile Golgi bodies that process and package ECM components into different sets of vesicles that traffic to the cortical cytoplasm, where they are transported around the cell by cytoplasmic streaming. At either fixed or transient areas, specific cytoplasmic vesicles fuse with the plasma membrane and secrete their constituents. Extracellular polysaccharide (EPS) production was observed to occur in one location of the Golgi body and sometimes in unique Golgi hybrids. Treatment of cells with brefeldin A caused disruption of the Golgi body, and inhibition of EPS secretion and cell wall expansion. The structure of the endomembrane system in Penium provides mechanistic insights into how extant charophytes generate large quantities of ECM, which in their ancestors facilitated the colonization of land.
Star–disc alignment in the protoplanetary discs: SPH simulation of the collapse of turbulent molecular cloud cores
Authors: Takaishi Daisuke , Tsukamoto Yusuke , Suto Yasushi 
Published: January 2020

ABSTRACT
We perform a series of three-dimensional smoothed particle hydrodynamics (SPH) simulations to study the evolution of the angle between the protostellar spin and the protoplanetary disc rotation axes (the star–disc angle ψsd) in turbulent molecular cloud cores. While ψsd at the protostar formation epoch exhibits broad distribution up to ∼130°, ψsd decreases (≲ 20°) in a time-scale of ∼104 yr. This time-scale of the star–disc alignment, talignment, corresponds basically to the mass doubling time of the central protostar, in which the protostar forgets its initial spin direction due to the mass accretion from the disc. Values of ψsd both at t = 102 yr and t = 105 yr after the protostar formation are independent of the ratios of thermal and turbulent energies to gravitational energy of the initial cloud cores: α = Ethermal/|Egravity| and γturb = Eturbulence/|Egravity|. We also find that a warped disc is possibly formed by the turbulent accretion flow from the circumstellar envelope.
Globular clusters in Coma cluster ultra-diffuse galaxies (UDGs): evidence for two types of UDG?
Authors: Forbes Duncan A , Alabi Adebusola , Romanowsky Aaron J , Brodie Jean P , Arimoto Nobuo 
Published: January 2020

ABSTRACT
Ultra-diffuse galaxies (UDGs) reveal extreme properties. Here, we compile the largest study to date of 85 globular cluster (GC) systems around UDGs in the Coma cluster, using new deep ground-based imaging of the known UDGs and existing imaging from the Hubble Space Telescope of their GC systems. We find that the richness of GC systems in UDGs generally exceeds that found in normal dwarf galaxies of the same stellar mass. These GC-rich UDGs imply haloes more massive than expected from the standard stellar mass–halo mass relation. The presence of such overly massive haloes presents a significant challenge to the latest simulations of UDGs in cluster environments. In some exceptional cases, the mass in the GC system is a significant fraction of the stellar content of the host galaxy. We find that rich GC systems tend to be hosted in UDGs of lower luminosity, smaller size, and fainter surface brightness. Similar trends are seen for normal dwarf galaxies in the Coma cluster. A toy model is presented in which the GC-rich UDGs are assumed to be ‘failed’ galaxies within massive haloes that have largely old, metal-poor, alpha-element-enhanced stellar populations. On the other hand, GC-poor UDGs are more akin to normal, low surface brightness dwarfs that occupy less massive dark matter haloes. Additional data on the stellar populations of UDGs with GC systems will help to further refine and test this simplistic model.
Constraining the gravitational-wave afterglow from a binary neutron star coalescence
Authors: Banagiri Sharan , Coughlin Michael W , Clark James , Lasky Paul D , Bizouard M A , Talbot Colm , Thrane Eric , Mandic Vuk 
Published: January 2020

ABSTRACT
Binary neutron star mergers are rich laboratories for physics, accessible with ground-based interferometric gravitational-wave detectors such as the Advanced LIGO and Advanced Virgo. If a neutron star remnant survives the merger, it can emit gravitational waves that might be detectable with the current or next generation detectors. The physics of the long-lived post-merger phase is not well understood and makes modelling difficult. In particular the phase of the gravitational-wave signal is not well modelled. In this paper, we explore methods for using long duration post-merger gravitational-wave signals to constrain the parameters and the properties of the remnant. We develop a phase-agnostic likelihood model that uses only the spectral content for parameter estimation and demonstrate the calculation of a Bayesian upper limit in the absence of a signal. With the millisecond magnetar model, we show that for an event like GW170817, the ellipticity of a long-lived remnant can be constrained to less than about 0.5 in the parameter space used.
Predictions for the 21cm-galaxy cross-power spectrum observable with SKA and future galaxy surveys
Authors: Vrbanec Dijana , Ciardi Benedetta , Jelić Vibor , Jensen Hannes , Iliev Ilian T , Mellema Garrelt , Zaroubi Saleem 
Published: January 2020

Abstract
In this paper we use radiative transfer + N-body simulations to explore the feasibility of measurements of cross-correlations between the 21 cm field observed by the Square Kilometer Array (SKA) and high-z Lyman Alpha Emitters (LAEs) detected in galaxy surveys with the Subaru Hyper Supreme Cam (HSC), Subaru Prime Focus Spectrograph (PFS) and Wide Field Infrared Survey Telescope (WFIRST). 21cm-LAE cross-correlations are in fact a powerful probe of the epoch of reionization as they are expected to provide precious information on the progress of reionization and the typical scale of ionized regions at different redshifts. The next generation observations with SKA will have a noise level much lower than those with its precursor radio facilities, introducing a significant improvement in the measurement of the cross-correlations. We find that an SKA-HSC/PFS observation will allow to investigate scales below ∼10 h−1 Mpc and ∼60 h−1 Mpc at z = 7.3 and 6.6, respectively. WFIRST will allow to access also higher redshifts, as it is expected to observe spectroscopically ∼900 LAEs per square degree and unit redshift in the range 7.5 ≤ z ≤ 8.5. Because of the reduction of the shot noise compared to HSC and PFS, observations with WFIRST will result in more precise cross-correlations and increased observable scales.