bluuebunny/crossref_metadata_2025_split huggingface dataset:
DNA Barcoding Reveals Cryptic Diversity in the Underestimated Genus Triplophysa (Cypriniformes: Cobitidae, Nemacheilinae) from the Northeastern Qinghai-Tibet Plateau
Authors: wang tai , Zhang Yanping , Yang Zhuoyu , Liu Zhe , Du Yanyan 
Published: September 2020

Abstract
Background: The northeastern part of the Qinghai-Tibet Plateau (QTP) is one of the areas where the number of species of plateau loach is the largest. As one of the three major groups of fishes distributed on the QTP, plateau loach have very important ecological value. However, their taxonomy and systematics are still controversial, and a large number of new species have been reported. The reason for this phenomenon is that the degree of morphological variation is low, the phylogenetic information provided by morphological and anatomical features used for species identification is relatively poor, and there are many cryptic species. Based on the high-density sampling points from the biodiversity hotspots surveyed, this study aims to evaluate the biodiversity of plateau loach in the northeastern of QTP and reveal the hidden diversity by comparing morphologic species with molecular operational taxonomic units (MOTUs). Results: After careful identification and comparison of the morphology and DNA barcoding of 1,630 specimens, 22 species were identified, 20 of which were considered valid local species and two of which were new species that had not been described. Based on the combination of morphological and molecular methods, a total of 24 native species have been found, two of which are cryptic species: Triplophysa robusta sp1 and Triplophysa minxianensis sp1. Fourteen of the 24 species form clusters of barcodes, which allow them to be reliably identified. The remaining cases involved 10 closely related species, some of which were rapidly differentiated, seemed to have experienced incomplete lineage sorting, or showed introgressions.Conclusions: The results highlight the need to combine traditional taxonomies with molecular methods to correctly identify species, especially in closely related species such as the plateau loach. This study provides a basis for protecting the biodiversity of plateau loach.
Evaluation of the Effect of Curcumin and Zinc Co-Supplementation on Glycemic Measurements, Lipid Profiles, Inflammatory and Antioxidant Biomarkers in Overweight or Obese Pre-Diabetic Patients: A study Protocol for a Randomized Double-Blind Placebo-Controlled Phase 2 Clinical Trial
Authors: Karandish Majid , Mozaffari-khosravi Hassan , Mohammadi Seyed Mohammad , cheraghian bahman , Azhdari Maryam 
Published: September 2020

Abstract
Background: The prevalence of prediabetes is increasing worldwide. Unfortunately, prediabetes is related to non-communicable diseases. A high risk of developing type 2 diabetes mellitus (T2DM) is reported in people with prediabetes. Curcumin, a polyphenol, might lead to its therapeutic role in obesity and some obesity-related metabolic diseases. Zinc is a trace element that plays a key role in the synthesis and action of insulin, carbohydrate metabolism, and decreasing inflammation. There has been no clinical trial of zinc and curcumin co-supplementation in patients with prediabetes. In previous studies, the single administration of zinc or curcumin hasn’t been conducted on many of the studied markers in pre-diabetic patients. Methods: The propose of this randomized double-blind placebo-controlled clinical trial is to investigate the effect of curcumin and zinc co-supplementation on glycemic measurements, lipid profiles, inflammatory and antioxidant biomarkers among 84 pre-diabetic patients with body mass index (BMI) between 25 and 35. Also, liver enzyme, serum zinc, urine zinc, blood pressure, anthropometric parameters, quality of life, adherence to co-supplementation, the side effects of co-supplementation, physical activity, and dietary intake will be assessed. Women or men (18-50 years old, for women: 18 years - before menopause) will be followed for three months (90 days). This study will be conducted at Yazd Diabetes Research Clinic, Shahid Sadoughi University of Medical Sciences. Discussion: A diet rich in antioxidants, polyphenols, and phytochemicals has been shown to have a beneficial role in prediabetes. According to the beneficial properties of curcumin or zinc and inadequate evidence, RCTs are needed to assess the effect of curcumin and zinc co-supplementation in native prediabetes patients. We hope the results of the present trial, negative or positive, fill this gap in the literature and facilitate the approach for a much larger, multi-center clinical trial. In conclusion, a synergic effect of co-supplementation along with a weight-loss diet may delay the progression to type 2 diabetes mellitus.Trial registration: The project is a registered clinical trial (Registration number: IRCT20190902044671N1, Iranian Registry of Clinical Trials (IRCT), registered October 11, 2019.
WITHDRAWN: Aging-related tumor associated fibroblasts changes could worsen the prognosis of GBM patients
Authors: Song Hongwang , Fu Xiaojun , Wu Chenxing , Li Shouwei 
Published: September 2020

Abstract

Background: Glioblastoma multiforme (GBM) is the most malignant tumor in human brain, with highly heterogeneity among different patients. Age could function as an incidence and prognosis risk factor for many tumors.Method: A series of bioinformatic experiments were conducted to evaluate the differences of incidence, differential expressed genes, enriched pathways with the data from Surveillance, Epidemiology, and End Results (SEER) program, the cancer genome atlas (TCGA) and Chinese glioma genome atlas (CGGA) project.Results: We discovered in our present study that distinct difference of incidence and prognosis of different aged GBM patients. By a series of bioinformatic method, we found that the tumor associated fibroblasts (TAFs) was the most crucial tumor microenvironment (TME) component that led to this phenomenon. Epithelial-mesenchymal transition (EMT) could be the mechanism by which TAFs regulate the progression of GBM. Conclusion: We have proposed a close correlation between age and GBM incidence and prognosis, and propose the underlying mechanism behind this correlation by mining different databases, which laid the foundation for future research.
Autosomal recessive polycystic kidney disease: case report of a newborn with rare PKHD1 mutation, rapid renal enlargement and early fatal outcome
Authors: Serra Gregorio , Corsello Giovanni , Antona Vincenzo , D'Alessandro Maria Michela , Cassata Nicola , Cimador Marcello , Giuffrè Mario , Schierz Ingrid Anne Mandy , Piro Ettore 
Published: September 2020

Abstract
Introduction: Autosomal recessive polycystic kidney disease (ARPKD; MIM#263200) is one of the most frequent pediatric renal cystic diseases, with an incidence of 1:20,000. It is caused by mutations of the PKHD1 gene, on chromosome 6p12. The clinical spectrum is highly variable, ranging from late-onset milder forms to severe perinatal manifestations. The management of newborns with severe pulmonary insufficiency is challenging, and causes of early death are sepsis or respiratory failure. In cases of massive renal enlargement, early bilateral nephrectomy and peritoneal dialysis may reduce infant mortality. However, there is no conclusive data on the role of surgery, and decision-making is driven by patient’s clinical condition and expertise of the center. Patient presentation: We hereby describe a preterm female newborn with perinatal, rapid and bilateral, abnormal growth of both kidneys, respiratory failure and initial signs of liver disease. She was subsequently confirmed to be affected by a rare and severe homozygous mutation of the PKHD1 gene, inherited from both her consanguineous parents. Our patient died 78 days after birth, due to a fungal sepsis which worsened her respiratory insufficiency. Conclusions: This patient report shows some of the clinical and ethical issues of neonatal ARPKD, and the need of multidisciplinary approach and good communication with the family. Target next generation sequencing (NGS) techniques may guide and support clinicians, as well as guarantee to these patients the most appropriate clinical management, avoiding unnecessary and/or disproportionate treatments.
Glycaemia and hand grip strength in aging people: Guangzhou Biobank Cohort Study
Authors: Liang Xue , Jiang Chao Qiang , Zhang Wei Sen , Zhu Feng , Jin Ya Li , Cheng Kar Keung , Lam Tai Hing , Xu Lin 
Published: September 2020

Abstract
BackgroundThere is a link between hyperglycemia and mechanical functions of muscle. However, existing evidence of the association between hyperglycemia and weaker muscle strength is limited and inconsistent. We examined whether glycemic status was associated with relative grip strength (RGS) in older Chinese.MethodsIn 2008-2012, 9180 participants (2516 men and 6664 women) from the Guangzhou Biobank Cohort Study had fasting and 2-hour post-load glucose measured. Glycemic status was categorized as normoglycaemia, prediabetes (i.e., impaired fasting glucose and/or impaired glucose tolerance) and diabetes. RGS was assessed using a Jamar Hydraulic Hand Dynamometer divided by body mass index. General linear model was used to assess the association of glycemic status with RGS.ResultsAfter adjusting for age, smoking status, alcohol use, physical activity, health status, body fat percentage and waist circumference, in men, hyperglycemia was associated with a lower RGS, with the RGS being 1.38 (95% confidence interval (CI)=1.34, 1.42) in normoglycaemia, 1.35 (95% CI=1.30, 1.39) in prediabetes, 1.33 (95% CI=1.29, 1.38) in newly diagnosed diabetes and 1.32 (95% CI=1.27, 1.37) in known diabetes (P for trend <0.001). The association of glycemic status with RGS was non-significant in women. Among the normoglycaemic group, no association was found between fasting glucose and RGS in men, whereas a significantly inverse association was found in women, with adjusted β for RGS per mmol/l increase in fasting glucose being -0.05 to -0.04 (P values from 0.002 to 0.03).ConclusionsHigher fasting glucose was associated with reduced grip strength in a dose-response manner, and the association was significant even in women with normoglycaemia. Our findings suggest that lowering glucose across the whole range might be important in preserving muscle strength, especially in aging women.
Infection Control and Management of COVID-19: Challenges for Paediatric Tertiary Care Hospitals
Authors: Remppis Jonathan , Ganzenmueller Tina , Vasconcelos Malte Kohns , Heinzel Oliver , Handgretinger Rupert , Renk Hanna 
Published: September 2020

Abstract
PURPOSE: To describe infection control measures and patient management at a tertiary children’s hospital in southern Germany during the COVID-19 pandemic.METHODS: Prospective, observational study of infection control measures, patient management, clinical and virologic data of paediatric patients treated at our hospital during the COVID-19 pandemic from February to May 2020. Infection control measures were documented beginning with preparation for the pandemic. All paediatric patients with suspected SARS-CoV-2 infection were prospectively included in the study.RESULTS: With local triage, restraint of patient admission and testing strategies implemented, healthcare capacity remained adequate and no healthcare-associated infections occurred. Workload in the paediatric emergency department significantly decreased following the lockdown of schools and kindergartens. 7 of 174 (4%) children with and 2 of 208 (1%) children without typical symptoms, respectively, were diagnosed with COVID-19 by PCR. Six out of nine inpatients treated for COVID-19 had underlying comorbidities, two were admitted to the intensive care unit. One patient died shortly after discharge.CONCLUSIONS: While COVID-19 generally causes mild disease in children, severe illness and fatal cases may occur, particularly among children with underlying diseases. Tertiary children’s hospitals may face challenges with treating potential high-risk patients during the pandemic. Thus, timely establishment of effective testing and triage strategies is crucial.
The Identification of Hub Genes and Pathways in type 2 Diabetes Mellitus by Bioinformatics Analysis
Authors: Li Jing , Jiang Xinkui , Chen Libo , Chen Hong 
Published: September 2020

Abstract
Background This study aimed to identify potential core genes and pathways involved in type 2 diabetes mellitus (T2DM) through exhaustive bioinformatics analysis. This study elucidated parts of the pathogenesis of T2DM and screened therapeutic targets of the treatment. Method: The original microarray data GSE25724 was downloaded from the Gene Expression Omnibus database. Data were processed by the limma package in R software and the differentially expressed genes(DEGs) were identified. Gene Ontology(GO) functional analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway analysis were carried out to identify potential biological functions and pathways of the DEGs. The STRING(Search Tool for the Retrieval of Interacting Genes ) and Cytoscape software were used to establish a protein-protein interaction(PPI) network for the DEGs.Hub genes were identified using the PPI network.Results In total, 75 DEGs were involved in T2DM, with 1 up-regulated gene, and 74 down-regulated genes. GO enrichment analysis showed that DEGs mainly enriched in the regulation of hormone levels, unfolded protein binding.KEGG pathway enrichment analysis showed that DEGs were significantly enriched in the fatty acid metabolism pathway, propionate metabolism pathway, and degradation pathway of valine, leucine, and isoleucine. Furthermore,Neuroendocrine protein 7B2(SCG5),Synaptosomal-associated protein 25 (SNAP25), Sterol Carrier Protein 2 (SCP2), Carboxypeptidase E (CPE),and Protein Convertase Subtilisin/Kexin Type 1 (PCSK1) were the core genes in the PPI network.Conclusion This study identified 5 hub genes as potential biomarkers of type 2 diabetes through bioinformatics analysis, which might increase our understanding of the potential molecular mechanisms of T2DM and provided targets for further research.
Evaluation frequency of Human Herpes Virus type 8 in Patients with Breast Cancer
Authors: Fozuni Elaheh , arabzadeh seyed Mohamad Ali , Mollaei Hamid Reza , Iranpour Maryam , Afshar Reza Malekpour 
Published: September 2020

Abstract
Background

Breast cancer is one of the most common malignancies and the most common cause of death women around worldwide. Recently, viral etiology theory has been proposed on physiopathology of the breast cancer.
Method:

In a retrospective study, to study of non-familial agents and a viral factors breast cancer, the Real time PCR were used to detect Kaposi Herpes virus or Human herpes virus type8(HHV8) in 138 patients with breast cancer.
Result

Out of the 138 embedded paraffin breast cancer tissues, 17(12.3%) were positive for HHV8. Among the viral positive breast cancer samples, 8 samples (10.95%) were positive in intermediate grade and 10 samples (11.9%) were positive in 40–60 years old age group. There was no significant relation between grades of tumors and there was a significant relation between age and breast cancer.
Conclusion

Out of 17 positive samples 15 samples (88.23%) and 2 samples (11.77%) had more than 5 and less than 3 infected lymph node. In this study, there was a significant relation between infected lymph node and positive samples with HHV-8. Therefore, it is likely that the lymphocytes infected with HHV-8 virus may be the source of cancer cells and may also cause infected cells to spread to other organs, as well as the treatment of these patients is difficult.
Validation of in vivo nodal staging of solid malignancies with USPIO-enhanced MRI: a workflow protocol
Authors: Driessen Daphne A.J.J. , Gouw Didi J.J.M. de , Stijns Rutger C.H. , Litjens Geke , Israël Bas , Philips Bart W.J. , Hermans John J. , Dijkema Tim , Klarenbeek Bastiaan R. , Post Rachel S. van der , Nagtegaal Iris D. , Grunsven Adriana C.H. van Engen-van , Brosens Lodewijk A.A. , Veltien Andor , Zámecnik Patrik , Scheenen Tom W.J. 
Published: September 2020

Abstract
BackgroundIn various cancer types, the first step towards extended metastatic disease is the presence of lymph node metastases. Imaging methods with sufficient diagnostic accuracy are required to personalize treatment. Lymph node metastases can be detected with ultrasmall superparamagnetic iron oxide (USPIO)-enhanced magnetic resonance imaging (MRI), but this method needs validation. Here, a workflow is presented which is designed to compare MRI-visible lymph nodes on a node-to-node basis with histopathology. MethodsIn patients with prostate, rectal, periampullary, esophageal, and head-and-neck cancer, in vivo USPIO-enhanced MRI was performed to detect lymph nodes suspicious for harboring metastases. After lymphadenectomy, but before histopathological assessment, a 7 Tesla (T) preclinical ex vivo MRI of the surgical specimen was performed, and in vivo MR-images were radiologically matched to ex vivo MR images. Lymph nodes were annotated on the ex vivo MRI for an MR-guided pathological examination of the specimens. ResultsMatching lymph nodes of ex vivo MRI to pathology was feasible in all cancer types. The annotated ex vivo MR images enabled a comparison between USPIO-enhanced in vivo MRI and histopathology which allowed for analyses on nodal, or at least on nodal station level.ConclusionsA workflow was developed to validate in vivo USPIO-enhanced MRI with histopathology. Guiding the pathologist towards lymph nodes in the resection specimens during histopathological work-up allowed analysis at nodal level, or at least nodal station level of in vivo suspicious lymph nodes with corresponding histopathology, providing direct information for validation of in vivo USPIO-enhanced MRI detected lymph nodes. Trial registrationVALINODE data is collected from patients undergoing USPIO-enhanced MRI in the clinical setting. Therefore, the trial has not been registered in an online trial register. 7TNANO1 is registered at Clinicaltrials.gov: NCT02751606, April 26 2016 (https://clinicaltrials.gov/ct2/show/NCT02751606). NANO-PANC is registered at Clinicaltrials.gov: NCT04311047, March 17 2020 (https://www.clinicaltrials.gov/ct2/show/NCT04311047). PRECIES is registered in the Dutch Trial Register: NTR6072, August 5 2016 (https://www.trialregister.nl/trial/5797). USPIO-NECK is registered at Clinicaltrials.gov: NCT03817307, January 25 2019 (https://clinicaltrials.gov/ct2/show/NCT03817307).
ZnT8 Loss-of-Function Accelerates Functional Maturation of hESC-Derived β Cells and Resists Metabolic Stress Induced Cell Death in Diabetes
Authors: Li Weida , Ma Qing , Xiao Yi-Ni , Li Sheng , Wang Menghan , Yang Zhihao , Xiao Tinghui , Xu Minglu , Zhang Tengjiao , Hu Rui , Zhang Zhen-Ning , He Qing , Su Qiang , Yuan Fei , Zhao jiaxu , Chen Zheng-Jun , Shi Weiyang , Deng Qiaolin , Cheng Xin 
Published: September 2020

Abstract
Human embryonic stem cells (hESCs) derived β cells (SC-β cells) hold great promise for diabetes treatment, yet how to achieve functional maturation of these SC-β cells and protect them against metabolic stresses such as glucotoxicity and lipotoxicity remain elusive. By single cell RNA-seq pseudotime analysis, we revealed that ZnT8 is involved in SC-β cells functional maturation process and its loss of function (LOF) accelerates functional maturation of SC-β cells. As a result, ZnT8 LOF improves glucose-stimulated insulin secretion (GSIS) and enhances proinsulin to insulin conversion efficiency in SC-β cells, both in vitro and in vivo, by releasing the negative feedback of zinc inhibition on insulin secretion. Furthermore, SC-β cells with ZnT8 LOF are resistant to metabolic stresses induced cell death, as lipotoxicity or glucotoxicity, displaying higher survival. Most importantly, transplantation of SLC30A8-/- SC-β cells into diabetic mice significantly improves glycemia restoration and SC-β cell survival with long-term stability. Therefore, our study offers an advanced cell replacement therapy for diabetes with both improved SC-β cell survival and function against metabolic stress.
Visual Barcodes for Multiplexing Live Microscopy-Based Assays
Authors: Kaufman Tom , Nitzan Erez , Firestein Nir , Ginzberg Miriam , Iyengar Seshu , Patel Nish , Ben-Hamo Rotem , Porat Ziv , Hilfinger Andreas , Kafri Ran , Straussman Ravid 
Published: September 2020

Abstract
While multiplexing samples using DNA barcoding revolutionized the pace of biomedical discovery, multiplexing of live imaging-based applications has been limited by the number of fluorescent proteins that can be deconvoluted using common microscopy equipment. To address this limitation we developed visual barcodes that discriminate the clonal identity of single cells by targeting different fluorescent proteins to specific subcellular locations. We demonstrate that deconvolution of these barcodes is highly accurate and robust to many cellular perturbations. We then used visual barcodes to generate ‘Signalome’ cell-lines by multiplexing live reporters to monitor the simultaneous activity in 12 branches of signaling, in live cells, at single cell resolution, over time. Using the ‘Signalome’ we identified two distinct clusters of signaling pathways that balance growth and proliferation, emphasizing the importance of growth homeostasis as a central organizing principle in cancer signaling. The ability to multiplex samples in live imaging applications, both in vitro and in vivo may allow better high-content characterization of complex biological system
Influence of Pre-Plasma on Accelerated Protons in Ultraintense Laser Interaction with Not-So-Thin Foils
Authors: Gizzi Leonida , Boella Elisabetta , Labate Luca , Baffigi Federica , Bilbao Pablo , Brandi Fernando , Cristoforetti Gabriele , Fazzi Alberto , Fulgentini Lorenzo , Giove Dario , Koester Petra , Palla Daniele , Tomassini Paolo 
Published: September 2020

Abstract
We report on recent experimental results on proton acceleration from laser interaction with foil targets at ultra-relativistic intensities. We show a three-fold increase in the proton cut-off energy when a micrometer scale-length pre-plasma is introduced by irradiation with a low energy femtosecond pre-pulse. The foil target is sufficiently thick to prevent disruption of the sheath field at the rear surface by the shock launched by the pre-pulse. Measurements are compared with accurate, numerical hydrodynamic and Particle-In-Cell simulations where the role of the finite plasma scale-length at the laser-target interface is taken into account and the role of stochastic heating in enhancing fast electron production is discussed.