bluuebunny/crossref_metadata_2025_split huggingface dataset:
Using intravenous pump infusion data to optimize continuous infusion concentrations and reduce drug and fluid waste
Authors: Forshay Charlotte M , Hansen Kevin N , Eckel Stephen F 
Published: August 2020

Abstract

Purpose
To outline a data-driven analysis involving use of intravenous (i.v.) pump data to identify optimal drug and fluid amounts for various continuous infusions, with the primary goal of minimizing medication waste. This methodology incorporates analysis of vial sizes, infusion rates, number of bag exchanges, and bag volumes to determine optimal concentrations that improve operational efficiencies and decrease drug and fluid waste.

Methods
A retrospective evaluation of i.v. infusion pump utilization data for continuous infusions of norepinephrine, phenylephrine, vasopressin, and cisatracurium was performed using data provided by 9 hospitals in North Carolina during January, April, and June of 2015.

Results
The recommended medication concentrations and fluid volumes were determined using a novel 4-step analysis, the VERB (Vial, Exchange, Rate, and Bag) analysis, which applied optimal practices for cost reduction, operational efficiency, and patient safety. The application of the VERB analysis to the i.v. infusion pump utilization data resulted in the following recommended medication concentrations: norepinephrine, 4 mg/100 mL (final concentration, 40 µg/mL) and 16 mg/250 mL (final concentration, 64 µg/mL); phenylephrine, 10 mg/100 mL (final concentration, 100 µg/mL); vasopressin, 20 units/100 mL (final concentration, 0.2 unit/mL); and cisatracurium, 200 mg/100 mL (final concentration, 2 mg/mL). It was determined that implementation of the recommended concentrations by the 9 study hospitals would result in significant medication cost savings and fluid volume savings.

Conclusion
Analysis of i.v. infusion pump data from multiple hospitals using VERB analysis resulted in standardized medication concentrations and bag sizes for continuous infusions that reduce drug and fluid waste and improve operational efficiencies.
Embryonic Exposure to Ethanol Increases Anxiety-Like Behavior in Fry Zebrafish
Authors: Pinheiro-da-Silva Jaquelinne , Agues-Barbosa Thais , Luchiari Ana Carolina 
Published: August 2020

AbstractAimsFetal alcohol spectrum disorder (FASD) is an umbrella term to describe the effects of ethanol (Eth) exposure during embryonic development, including several conditions from malformation to cognitive deficits. Zebrafish (Danio rerio) are a translational model popularly applied in brain disorders and drug screening studies due to its genetic and physiology homology to humans added to its transparent eggs and fast development. In this study, we investigated how early ethanol exposure affects zebrafish behavior during the initial growth phase.MethodsFish eggs were exposed to 0.0 (control), 0.25 and 0.5% ethanol at 24 h post-fertilization. Later, fry zebrafish (10 days old) were tested in a novel tank task and an inhibitory avoidance protocol to inquire about morphology and behavioral alterations.ResultsAnalysis of variance showed that ethanol doses of 0.25 and 0.5% do not cause morphological malformations and did not impair associative learning but increased anxiety-like behavior responses and lower exploratory behavior when compared to the control.ConclusionOur results demonstrate that one can detect behavioral abnormalities in the zebrafish induced by embryonic ethanol as early as 10 days post-fertilization and that alcohol increases anxious behavior during young development in zebrafish.
Seed size, number and strategies in annual plants: a comparative functional analysis and synthesis
Authors: Hodgson John G , Montserrat Marti Gabriel , Šerá Bozena , Jones Glynis , Bogaard Amy , Charles Mike , Font Xavier , Ater Mohammed , Taleb Abdelkader , Santini Bianca A , Hmimsa Younes , Palmer Carol , Wilson Peter J , Band Stuart R , Styring Amy , Diffey Charlotte , Green Laura , Nitsch Erika , Stroud Elizabeth , Warham Gemma 
Published: August 2020

AbstractBackground and AimsPlants depend fundamentally on establishment from seed. However, protocols in trait-based ecology currently estimate seed size but not seed number. This can be rectified. For annuals, seed number should simply be a positive function of vegetative biomass and a negative function of seed size.MethodsUsing published values of comparative seed number as the ‘gold standard’ and a large functional database, comparative seed yield and number per plant and per m2 were predicted by multiple regression. Subsequently, ecological variation in each was explored for English and Spanish habitats, newly calculated C-S-R strategies and changed abundance in the British flora.Key ResultsAs predicted, comparative seed mass yield per plant was consistently a positive function of plant size and competitive ability, and largely independent of seed size. Regressions estimating comparative seed number included, additionally, seed size as a negative function. Relationships differed numerically between regions, habitats and C-S-R strategies. Moreover, some species differed in life history over their geographical range. Comparative seed yield per m2 was positively correlated with FAO crop yield, and increasing British annuals produced numerous seeds. Nevertheless, predicted values must be viewed as comparative rather than absolute: they varied according to the ‘gold standard’ predictor used. Moreover, regressions estimating comparative seed yield per m2 achieved low precision.ConclusionsFor the first time, estimates of comparative seed yield and number for &gt;800 annuals and their predictor equations have been produced and the ecological importance of these regenerative traits has been illustrated. ‘Regenerative trait-based ecology’ remains in its infancy, with work needed on determinate vs. indeterminate flowering (‘bet-hedging’), C-S-R methodologies, phylogeny, comparative seed yield per m2 and changing life history. Nevertheless, this has been a positive start and readers are invited to use estimates for &gt;800 annuals, in the Supplementary data, to help advance ‘regenerative trait-based ecology’ to the next level.
MELODI Presto: a fast and agile tool to explore semantic triples derived from biomedical literature
Authors: Elsworth Benjamin , Gaunt Tom R 
Published: August 2020

ABSTRACT

Summary
The field of literature-based discovery is growing in step with the volume of literature being produced. From modern natural language processing algorithms to high quality entity tagging, the methods and their impact are developing rapidly. One annotation object that arises from these approaches, the subject–predicate–object triple, is proving to be very useful in representing knowledge. We have implemented efficient search methods and an application programming interface, to create fast and convenient functions to utilize triples extracted from the biomedical literature by SemMedDB. By refining these data, we have identified a set of triples that focus on the mechanistic aspects of the literature, and provide simple methods to explore both enriched triples from single queries, and overlapping triples across two query lists.

Availability and Implementation
https://melodi-presto.mrcieu.ac.uk/.

Supplementary information
Supplementary data are available at Bioinformatics online.
Traditional Horticulture Practices Increase the Production of Selected Withanolides in Withania Somnifera (L.) Dunal—A RP-UFLC Analysis
Authors: Ankad Gireesh M , Pai Sandeep R , Hiremath Jagadishchandra , Hegde Harsha V 
Published: August 2020

Abstract
The study evaluates the effect of two traditional horticulture treatments mentioned in Vrikshayurveda, a text from ancient India on the science of plant life, namely Kunapa jala (KJ) and Pancha gavya (PG) on the production of Withaferin A (WFA), withanolide A (WIA) and Withanolide B (WIB) in Withania somnifera (L) Dunal. Leaves and roots of W. somnifera were collected from different treated groups viz. control, KJ, PG, farmyard manure (FYM) and inorganic fertilizer (NPK). Reverse phase ultra-flow liquid chromatography (RP-UFLC) method was developed, validated for simultaneous detection of WFA, WIA and WIB. Statistical analysis of data was performed by ANOVA and tested for significance by the Dunnett multiple comparison test and data were expressed as mean ± standard deviation (SD). Results revealed, leaves possessed highest WFA content and roots possessed highest content of WIA and WIB. PG treated leaves were observed highest WFA (18.29 mg/g) and roots were observed highest WIA (19.63 mg/g) and WIB (1.36 mg/g). Conclusively, RP-UFLC method for simultaneous detection of withanolides has been developed and validated to evaluate the effect of traditional horticulture treatments. It is concluded that the enhanced production of withanolides can be achieved by the application of PG when compared to NPK application.
Towards an epistemology for conjunctural inter-urban comparison
Authors: Leitner Helga , Sheppard Eric 
Published: August 2020

AbstractWe propose an epistemology for conjunctural inter-urban comparison, stressing the dialectical relationship between the general and the particular. We spatialise conjunctural analysis, avoiding methodological territorialism by extending the explanatory framework outwards in space to incorporate inter-territorial connections and supra-territorial scalar relations. We then provide three guiding principles for conjunctural comparison: an open starting point, a three-dimensional socio-spatial ontology and the general/particular dialectic. Illustrating this with comparative fieldwork on urban land transformations in Jakarta and Bangalore, we stress-test received theories and develop Inter-scalar Chains of Rentiership: this mid-range concept clarifies shared tendencies across the cities, particularities differentiating them and their inter-relations.
Pyrrhonian Scepticism in Thomas Elyot'sDefence of Good Women(1540)
Authors: Gligorijevic Kosta 
Published: August 2020

AbstractThis article discusses the first known mention of Pyrrhonian Scepticism in the English language, which occurs in Thomas Elyot’s 1540 Defence of Good Women. The article inquires into the sources of Elyot’s knowledge of Pyrrhonism and into his motivations for mentioning Pyrrhonists in a text on the social and political status of women. I conclude that, on the balance of evidence, the most likely source of Elyot’s ideas on Pyrrhonism is Galen’s De Temperamentis. Furthermore, I argue that the rejection of a straw man version of Pyrrhonism serves as a tool of authorial positioning for Elyot, and in particular as a means of reassuring his reader that the Defence is not intended ironically. Elyot chooses Pyrrhonism as a philosophical position representing bad faith arguments, and he likely does so because of the hostile treatment of Pyrrhonism in Galen’s works, and especially in De Temperamentis.
Coronavirus disease 2019 (COVID-19) in the heart transplant population: a single-centre experience
Authors: Caraffa Raphael , Bagozzi Lorenzo , Fiocco Alessandro , Bifulco Olimpia , Nadali Matteo , Ponzoni Matteo , Carrozzini Massimiliano , Toscano Giuseppe , Fraiese Angela Pompea , Metra Marco , Lombardi Carlo Maria , Serafini Francesco , Ribola Angela , Jorgji Vjola , Bottio Tomaso , Gerosa Gino 
Published: August 2020

AbstractOBJECTIVESFew anecdotal cases have been reported in the literature regarding heart transplant recipients and infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). We report our experience with 6 patients hospitalized in Northern Italy during the outbreak.METHODSOf the 396 living heart transplant recipients from 1985 to 2020 included in the study, 6 patients developed the novel 2019 coronavirus disease. Risk factors, last follow-up characteristics, onset presentation, in-hospital course of disease and blood examinations data were collected for these patients.RESULTSAll patients were symptomatic and had positive results from a nasopharyngeal swab test for SARS-CoV-2. Of the 6 patients, 5 were hospitalized and 1 remained self-quarantined at home. Two patients died and 3 were discharged home. Two patients were admittted to the intensive care unit . Immunosuppressive therapy was modified with a median reduction comprising doses that were 50% cyclosporine and 50% mycophenolate. All patients received a medium-dose of corticosteroids as a bolus medication in addition to their therapy. All hospitalized patients received hydroxychloroquine; 2 patients received ritonavir/lopinavir. Broad-spectrum antibiotics for prophylaxis were administered to all. One patient had an ischaemic stroke and died of sepsis.CONCLUSIONSIn the absence of any strong evidence regarding the treatment of heart transplant recipients infected with SARS-CoV-2, we faced a new challenge in managing viral infection in an immunosuppressed population. Because immunomodulation interaction with the infection seems to be crucial for developing severe forms of the disease, we managed to reduce immunosuppressive therapy by adding medium doses of corticosteroids. Despite the limited number of affected patients, this report suggests that special considerations should be given to treating coronavirus disease in the heart transplant recipient population.
Organizing talk in group speaking tests: learning from high-scoring students
Authors: Stephenson Michael , Hall Graham 
Published: August 2020

Abstract
This article investigates how group speaking test candidates manage group talk during assessed, time-limited tasks. Drawing on recorded and transcribed data, it explores how higher-scoring learners manage spoken assessment tasks through their talk, moving conversations on or slowing them down in order to perform and complete the tasks’ requirements in a timely way. These data are contrasted with the ways in which lower-scoring test candidates attempt to organize and manage assessed group talk. While the data and findings from this particular study can be used to teach students about, and prepare them for, multi-person discussions and, more specifically, task-oriented and time-limited group speaking tests, we also suggest that our awareness-raising investigatory approach, if brought into the classroom, offers learners (and teachers) a way of uncovering some of what actually occurs during talk, illuminating the ‘invisible rules’ that guide how participants engage in and manage interaction.
Bacterial and fungal diversity in the lorandite (TlAsS2) mine ‘Allchar' in the Republic of North Macedonia
Authors: Taleski Vaso , Dimkić Ivica , Boev Blazo , Boev Ivan , Živković Sanja , Stanković Slaviša 
Published: August 2020

ABSTRACT
The Allchar mineral mine is one of the oldest arsenic–antimony mines in the Republic of North Macedonia. The mine is a well-known reservoir of the worldwide purest source of the thallium-bearing mineral, lorandite (TlAsS2). The current study evaluated the bacterial and fungal diversity of three As- and Tl-contaminated sites in Allchar mineral mine. We used a combination of high-throughput sequencing and bioinformatic analyses. Trace metal content was detected using inductively coupled plasma optical emission spectrometry. Our analysis showed the presence of 25 elements and confirmed a high concentration of As and Tl. Alpha diversity indices suggested a high diversity and evenness of bacterial and fungal communities. Bacterial phyla that dominated the environment were Bacteroidetes, Acidobacteria, Planctomycetes, Actinobacteria and Verrucomicrobia. Looking at the genus level, we found the following groups of bacteria: Chryseolinea, Opitutus, Flavobacterium, Pseudomonas, Terrimonas, Sphingomonas and Reyranella. For the fungi genera, we report Tetracladium sp., Coprinellus micaceus, Coprinus sp. from Ascomycota and Basidiomycota phyla in all sites. We also observed a high abundance of the fungal species Pilidium sp., Dendroclathra lignicola, Rosellinia desmazieri, Hypomyces rosellus and Coprinellus disseminatus. This study is the first to identify specific As- and Tl-tolerant fungal (Pilidium sp., Cladophialophora sp., Neobulgaria sp. and Mycena acicula) and bacterial (Trichococcus, Devosia, Litorilinea and Gimesia) genera from Allchar mine, suggesting bioremediation and industrial potential.
Structurally complex carbohydrates maintain diversity in gut-derived microbial consortia under high dilution pressure
Authors: Yao Tianming , Chen Ming-Hsu , Lindemann Stephen R 
Published: August 2020

ABSTRACTDietary fibers are major substrates for maintaining and shaping gut microbiota, but the structural specificity of these fibers for the diversity, structure and function of gut microbiota are poorly understood. Here, we employed an in vitro sequential batch fecal culture approach to address two ecological questions: (i) whether the chemical complexity of a carbohydrate influences its ability to maintain microbial diversity against high dilution pressure (ii) whether substrate structuring or obligate microbe-microbe metabolic interactions (e.g. exchange of amino acids or vitamins) exert more influence on maintained diversity. Sorghum arabinoxylan (SAX, a complex polysaccharide), inulin (a low-complexity oligosaccharide) and their corresponding monosaccharide controls were selected as model carbohydrates. Our results demonstrate that complex carbohydrates stably sustain diverse microbial consortia. Furthermore, other metabolic interactions were less influential in structuring microbial consortia consuming SAX than inulin. Finally, very similar final consortia were enriched on SAX from the same individual's fecal microbiota one month later, suggesting that polysaccharide structure is more influential than stochastic alterations in microbiome composition in governing the outcomes of sequential batch cultivation experiments. These data suggest that carbohydrate structural complexity affords independent niches that structure fermenting microbial consortia, whereas other metabolic interactions govern the composition of communities fermenting simpler carbohydrates.
Mucoidy, a general mechanism for maintaining lytic phage in populations of bacteria
Authors: Chaudhry Waqas , Lee Esther , Worthy Andrew , Weiss Zoe , Grabowicz Marcin , Vega Nicole , Levin Bruce 
Published: August 2020

ABSTRACT
We present evidence that phage resistance resulting from overproduction of exopolysaccharides, mucoidy, provides a general answer to the longstanding question of how lytic viruses are maintained in populations dominated by bacteria upon which they cannot replicate. In serial transfer culture, populations of mucoid Escherichia coli MG1655 that are resistant to lytic phages with different receptors, and thereby requiring independent mutations for surface resistance, are capable of maintaining these phages with little effect on their total density. Based on the results of our analysis of a mathematical model, we postulate that the maintenance of phage in populations dominated by mucoid cells can be attributed primarily to high rates of transition from the resistant mucoid states to susceptible non-mucoid states. Our tests with both population dynamic and single cell experiments as well as genomic analysis are consistent with this hypothesis. We discuss reasons for the generalized resistance of these mucoid E. coli, and the genetic and molecular mechanisms responsible for the high rate of transition from mucoid to sensitive states responsible for the maintenance of lytic phage in mucoid populations of E. coli.
The Effects of Autocratic Characteristics on Public Opinion toward Democracy Promotion Policies: A Conjoint Analysis
Authors: Escribà-Folch Abel , Muradova Lala H , Rodon Toni 
Published: August 2020

Abstract
Does the level of public support for democracy promotion policies vary with the characteristics of potential autocratic targets? We conduct an experimental study with a conjoint design on a sample of 1,464 US citizens that manipulates several core characteristics of potential autocratic targets. We then compare citizens’ preferences with the cross-national evidence testing the determinants of democracy promotion success. We find that respondents support the use of coercive measures (military action and sanctions) precisely in contexts where, according to comparative research, these instruments are unlikely to foster democratization: oil-rich, exclusionary, personalistic regimes with no elections, and with no ties to the United States. Conversely, the characteristics driving public support for the use of democracy aid are more consistent with those favoring effectiveness: autocratic regimes with multi-party elections and with links to the United States. These findings have important policy implications by contributing to understand the micro-foundations of target selection.
Microhomologies Are Associated with Tandem Duplications and Structural Variation in Plant Mitochondrial Genomes
Authors: Xia Hanhan , Zhao Wei , Shi Yong , Wang Xiao-Ru , Wang Baosheng 
Published: August 2020

AbstractShort tandem repeats (STRs) contribute to structural variation in plant mitochondrial genomes, but the mechanisms underlying their formation and expansion are unclear. In this study, we detected high polymorphism in the nad7-1 region of the Pinus tabuliformis mitogenome caused by the rapid accumulation of STRs and rearrangements over a few million years ago. The STRs in nad7-1 have a 7-bp microhomology (TAG7) flanking the repeat array. We then scanned the mitogenomes of 136 seed plants to understand the role of microhomology in the formation of STR and mitogenome evolution. A total of 13,170 STRs were identified, and almost half of them were associated with microhomologies. A substantial amount (1,197) of microhomologies was long enough to mediate structural variation, and the length of microhomology is positively correlated with the length of tandem repeat unit. These results suggest that microhomology may be involved in the formation of tandem repeat via microhomology-mediated pathway, and the formation of longer duplicates required greater length of microhomology. We examined the abundance of these 1,197 microhomologies, and found 75% of them were enriched in the plant mitogenomes. Further analyses of the 400 prevalent microhomologies revealed that 175 of them showed differential enrichment between angiosperms and gymnosperms and 186 differed between angiosperms and conifers, indicating lineage-specific usage and expansion of microhomologies. Our study sheds light on the sources of structural variation in plant mitochondrial genomes and highlights the importance of microhomology in mitochondrial genome evolution.
Expression Evolution of Ancestral XY Gametologs across All Major Groups of Placental Mammals
Authors: Martínez-Pacheco Mónica , Tenorio Mariela , Almonte Laura , Fajardo Vicente , Godínez Alan , Fernández Diego , Cornejo-Páramo Paola , Díaz-Barba Karina , Halbert Jean , Liechti Angelica , Székely Tamas , Urrutia Araxi O , Cortez Diego 
Published: August 2020

AbstractPlacental mammals present 180 million-year-old Y chromosomes that have retained a handful of dosage-sensitive genes. However, the expression evolution of Y-linked genes across placental groups has remained largely unexplored. Here, we expanded the number of Y gametolog sequences by analyzing ten additional species from previously unexplored groups. We detected seven remarkably conserved genes across 25 placental species with known Y repertoires. We then used RNA-seq data from 17 placental mammals to unveil the expression evolution of XY gametologs. We found that Y gametologs followed, on average, a 3-fold expression loss and that X gametologs also experienced some expression reduction, particularly in primates. Y gametologs gained testis specificity through an accelerated expression decay in somatic tissues. Moreover, despite the substantial expression decay of Y genes, the combined expression of XY gametologs in males is higher than that of both X gametologs in females. Finally, our work describes several features of the Y chromosome in the last common mammalian ancestor.