bluuebunny/crossref_metadata_2025_split huggingface dataset:
Motor neuron preservation and decrease of in vivo TDP-43 phosphorylation by protein CK-1δ kinase inhibitor treatment
Authors: Martínez-González Loreto , Rodríguez-Cueto Carmen , Cabezudo Diego , Bartolomé Fernando , Andrés-Benito Pol , Ferrer Isidro , Gil Carmen , Martín-Requero Ángeles , Fernández-Ruiz Javier , Martínez Ana , de Lago Eva 
Published: March 2020

AbstractPathogenesis of amyotrophic lateral sclerosis (ALS), a devastating disease where no treatment exists, involves the compartmentalization of the nuclear protein TDP-43 (TAR DNA-binding protein 43) in the cytoplasm which is promoted by its aberrant phosphorylation and others posttranslational modifications. Recently, it was reported that CK-1δ (protein casein kinase-1δ) is able to phosphorylate TDP-43. Here, the preclinical efficacy of a benzothiazole-based CK-1δ inhibitor IGS-2.7, both in a TDP-43 (A315T) transgenic mouse and in a human cell-based model of ALS, is shown. Treatment with IGS-2.7 produces a significant preservation of motor neurons in the anterior horn at lumbar level, a decrease in both astroglial and microglial reactivity in this area, and in TDP-43 phosphorylation in spinal cord samples. Furthermore, the recovery of TDP-43 homeostasis (phosphorylation and localization) in a human-based cell model from ALS patients after treatment with IGS-2.7 is also reported. Moreover, we have shown a trend to increase in CK-1δ mRNA in spinal cord and significantly in frontal cortex of sALS cases. All these data show for the first time the in vivo modulation of TDP-43 toxicity by CK-1δ inhibition with IGS-2.7, which may explain the benefits in the preservation of spinal motor neurons and point to the relevance of CK-1δ inhibitors in a future disease-modifying treatment for ALS.
Plasma B-type natriuretic peptide cannot predict treatment response to ibuprofen in preterm infants with patent ductus arteriosus
Authors: Oh Seong Hee , Lee Byong Sop , Jung Euiseok , Oh Moon Yeon , Do Hyun-Jeong , Kim Ellen Ai-Rhan , Kim Ki-Soo 
Published: March 2020

AbstractPlasma B-type natriuretic peptide (BNP) is a useful marker for diagnosis of hemodynamically significant PDA (hsPDA) and serial BNP measurement is also valuable for monitoring treatment response. This retrospective study was performed to evaluate whether plasma BNP level can predict treatment response to ibuprofen in preterm infants born at <30 weeks of gestation with hsPDA. Plasma BNP was measured before (baseline) and 12 to 24 h after (post-treatment) completion of the first (IBU1) and second (IBU2) course of ibuprofen. We compared the BNP levels of responders (closed or insignificant PDA) with those of non-responders (hsPDA requiring further pharmacologic or surgical closure) to each course of ibuprofen. The treatment response rates for IBU1 (n = 92) and IBU2 (n = 19) were 74% and 26%, respectively. In IBU1, non-responders had lower gestational age and birth weight than responders (both, P = 0.004), while in IBU2, non-responders had lower birth weight (P = 0.014) and platelet counts (P = 0.005) than responders; however, baseline BNP levels did not differ significantly between responders and non-responders in either IBU1 (median 1,434 vs. 1,750 pg/mL) or IBU2 (415 vs. 596 pg/mL). Post-treatment BNP was a useful marker for monitoring treatment efficacy of IBU1 and IBU2 for hsPDA with a cut-off value of 331 pg/mL (P < 0.001) and 423 pg/mL(P < 0.010), respectively. We did not identify a cut-off baseline BNP level that could predict treatment response to ibuprofen in preterm infants with hsPDA.
A GIS-based 3D slope stability analysis method based on the assumed normal stress on the slip surface
Authors: Yu Guo , Xie Mowen , Liang Jun , Farooq Asim , Williams Edward J. 
Published: March 2020

AbstractThe study proposes a geographic information systems (GIS)-based slope stability analysis method assuming a normal stress distribution acting on the slip surface. Compared with traditional methods, the three-dimensional (3D) safety factor acquired through this method will more closely approximate the actual value. First, a 3D slope stability analysis model is developed using grid column units, and the spatial expression of calculation parameters based on the grid column is given by the spatial analysis capability of GIS. Then, four equilibrium equations are derived under the limit equilibrium condition. The normal stress distribution acting on the slip surface is analyzed to construct a reasonable normal stress distribution approximation function. The 3D safety factor is obtained through the approximation function and the Mohr-Coulomb strength criterion. Moreover, we develop a GIS-based extension module which combines the grid-based data with the 3D slope stability analysis model. The accuracy and feasibility of the module are verified by three typical cases.
Eosinophil accumulation in postnatal lung is specific to the primary septation phase of development
Authors: Loffredo Lucas F. , Coden Mackenzie E. , Jeong Brian M. , Walker Matthew T. , Anekalla Kishore Reddy , Doan Ton C. , Rodriguez Raul , Browning Mandy , Nam Kiwon , Lee James J. , Abdala-Valencia Hiam , Berdnikovs Sergejs 
Published: March 2020

AbstractType 2 immune cells and eosinophils are transiently present in the lung tissue not only in pathology (allergic disease, parasite expulsion) but also during normal postnatal development. However, the lung developmental processes underlying airway recruitment of eosinophils after birth remain unexplored. We determined that in mice, mature eosinophils are transiently recruited to the lung during postnatal days 3–14, which specifically corresponds to the primary septation/alveolarization phase of lung development. Developmental eosinophils peaked during P10-14 and exhibited Siglec-Fmed/highCD11c−/low phenotypes, similar to allergic asthma models. By interrogating the lung transcriptome and proteome during peak eosinophil recruitment in postnatal development, we identified markers that functionally capture the establishment of the mesenchymal-epithelial interface (Nes, Smo, Wnt5a, Nog) and the deposition of the provisional extracellular matrix (ECM) (Tnc, Postn, Spon2, Thbs2) as a key lung morphogenetic event associating with eosinophils. Tenascin-C (TNC) was identified as one of the key ECM markers in the lung epithelial-mesenchymal interface both at the RNA and protein levels, consistently associating with eosinophils in development and disease in mice and humans. As determined by RNA-seq analysis, naïve murine eosinophils cultured with ECM enriched in TNC significantly induced expression of Siglec-F, CD11c, eosinophil peroxidase, and other markers typical for activated eosinophils in development and allergic inflammatory responses. TNC knockout mice had an altered eosinophil recruitment profile in development. Collectively, our results indicate that lung morphogenetic processes associated with heightened Type 2 immunity are not merely a tissue “background” but specifically guide immune cells both in development and pathology.
Arteriovenous Malformation MAP2K1 Mutation Causes Local Cartilage Overgrowth by a Cell-Non Autonomous Mechanism
Authors: Konczyk Dennis J. , Goss Jeremy A. , Smits Patrick J. , Sudduth Christopher L. , Al-Ibraheemi Alyaa , Greene Arin K. 
Published: March 2020

AbstractExtracranial arteriovenous malformation (AVM) is most commonly caused by MAP2K1 mutations in the endothelial cell. The purpose of this study was to determine if local tissue overgrowth associated with AVM is caused by direct or indirect effects of the MAP2K1 mutation (i.e., cell-autonomous or cell-non autonomous). Because cartilage does not have blood vessels, we studied ear AVMs to determine if overgrown cartilage contained AVM-causing mutations. Cartilage was separated from its surrounding tissue and isolated by laser capture microdissection. Droplet digital PCR (ddPCR) was used to identify MAP2K1 mutations. MAP2K1 (p.K57N) variants were present in the tissue adjacent to the cartilage [mutant allele frequency (MAF) 6–8%], and were enriched in endothelial cells (MAF 51%) compared to non-endothelial cells (MAF 0%). MAP2K1 mutations were not identified in the overgrown cartilage, and thus local cartilage overgrowth likely results from the effects of adjacent mutant blood vessels (i.e., cell-non autonomous).
Pharmacological inhibition of ataxia-telangiectasia mutated exacerbates acute kidney injury by activating p53 signaling in mice
Authors: Uehara Masahiro , Kusaba Tetsuro , Ida Tomoharu , Nakai Kunihiro , Nakata Tomohiro , Tomita Aya , Watanabe-Uehara Noriko , Ikeda Kisho , Kitani Takashi , Yamashita Noriyuki , Kirita Yuhei , Matoba Satoaki , Humphreys Benjamin D. , Tamagaki Keiichi 
Published: March 2020

AbstractThe DNA damage response after kidney injury induces cell cycle arrest in renal tubular epithelial cells, resulting in the secretion of pro-fibrotic cytokines, thereby promoting interstitial fibrosis in a paracrine manner. Phosphorylation of ataxia-telangiectasia mutated (ATM) is the initial step in the DNA damage response and subsequent cell cycle arrest; however, the effects of ATM inhibition on the injured kidney have not been explored. Pharmacological ATM inhibition by KU55933 in cisplatin-treated mice did not ameliorate, but instead exacerbated cisplatin-induced DNA damage and tubular injury, thereby increasing mortality. Analysis of isolated tubular epithelia by FACS from bigenic SLC34a1-CreERt2; R26tdTomato proximal tubular-specific reporter mice revealed that KU55933 upregulated p53 and subsequent pro-apoptotic signaling in tubular epithelia of cisplatin-treated mice, leading to marked mitochondrial injury and apoptosis. In addition, KU55933 attenuated several DNA repair processes after cisplatin treatment, including single-strand DNA repair and Fanconi anemia pathways, suggesting that DNA repair after dual treatment of cisplatin and KU55933 was not sufficient to prevent the cisplatin-induced tubular injury. Our study suggested that ATM inhibition does not increase DNA repair after cisplatin-induced DNA damage and exacerbates tubular injury through the upregulation of p53-dependent pro-apoptotic signaling. Acute kidney injury must be carefully monitored when ATM inhibitors become available in clinical practice in the future.
Simultaneous effect of strain rate and humidity on the structure and mechanical behavior of spider silk
Authors: Yazawa Kenjiro , Malay Ali D. , Masunaga Hiroyasu , Norma-Rashid Y. , Numata Keiji 
Published: March 2020

AbstractSpider dragline silk fibers are important in nature for capturing prey and as a lifeline. However, spider silk is exposed to a range of humidity and deformation conditions, and it is important to understand what effect these have on its properties. Here, we simultaneously investigated the effect of a wide range of strain rates on the structural and mechanical properties of spider silk under different humidity conditions. The toughness of the silk fiber was enhanced under mild humidity and high deformation rate conditions, which occur in the natural habitat of spiders. Structural changes in the fiber upon tension were monitored with a wide-angle X-ray scattering system, showing that during stretching the orientation of the crystalline β-sheets aligned, whereas the crystallite size decreased. These findings help to understand the link between the structural changes and mechanical behavior of spider silk.
Gas fractions and depletion times in galaxies with different degrees of interaction
Authors: Díaz-García S. , Knapen J. H. 
Published: March 2020

Context. A moderate enhancement of the star formation rates (SFR) in local interacting galaxies has been reported, but the physical mechanisms leading to this increase are not clear.
Aims. We study the atomic gas content and the central stellar mass concentration for a sample of almost 1500 nearby galaxies to further investigate the nature of starbursts and the influence of galaxy-galaxy interactions on star formation.
Methods. We used a sample of catalogued interacting and non-interacting galaxies in the S4G survey – along with archival H I gas masses, stellar masses (M*), and SFRs from IRAS far-infrared fluxes – and calculate depletion times (τ) and gas fractions. We traced the central stellar mass concentration from the inner slope of the stellar component of the rotation curves, dRv*(0). Starbursts are defined as galaxies with a factor > 4 enhanced SFR relative to a control sample of non-interacting galaxies which are ±0.2 dex in stellar mass and ±1 in T-type.
Results. Starbursts are mainly early-type (T ≲ 5), massive spiral galaxies (M* ≳ 1010 M⊙) that are not necessarily interacting. For a given stellar mass bin, starbursts are characterised by lower gas depletion times, similar gas fractions, and larger central stellar mass concentrations than non-starburst galaxies. The global distributions of gas fraction and gas depletion time of interacting galaxies are not statistically different from those of their non-interacting counterparts. However, in the case of currently merging galaxies, the median gas depletion time is a factor of 0.4 ± 0.2 that of control sample galaxies, and their SFRs are a factor of 1.9 ± 0.5 enhanced, even though the median gas fraction is similar.
Conclusions. Starbursts present long-lasting star formation in circumnuclear regions, which causes an enhancement of the central stellar density at z ≈ 0 in both interacting and non-interacting systems. Starbursts have low gas depletion timescales, yet similar gas fractions as normal main-sequence galaxies. Galaxy mergers cause a moderate enhancement of the star formation efficiency.
Overcoming Knowledge Barriers for Inclusion of School-Based Speech-Language Pathologists in the Management of Students with Mild Traumatic Brain Injury
Authors: O'Brien Katy H. 
Published: March 2020

AbstractSchool-based speech-language pathologists (SLPs) can play an important role in the recovery of children who have sustained a mild traumatic brain injury (mTBI). Two types of knowledge barriers are described here that impact the beliefs and ability of SLPs to respond to the needs of these students. Foundational knowledge is relatively stable over time, and encompasses basic definitions and understanding of the injury. In contrast, flexible knowledge addresses clinical management, including assessment and treatment, and should be regularly updated to align with current best practice recommendations. Clinicians are sensitive to this difference, seeking clinically applicable continuing education. However, general poor understanding of pediatric mTBI paired with rapidly advancing research in the field has led to widespread inaccuracies in both foundational and flexible knowledge. Suggestions are provided for educational initiatives and for advocacy of the role of SLPs in the care of students with mTBI.
Development of a scale of Chinese primary school teachers’ job embeddedness
Authors: Sun Xuguang , Huang Ailing 
Published: March 2020

AbstractBased on the background of Chinese education reform and Chinese cultural values, the paper aims to develop a good reliable and valid scale to measure the job embeddedness of Chinese primary school teachers. To achieve the above purpose, first, we created these domains and items through an analysis of previous literature and an opened questionnaire survey of Chinese primary school teachers. Second, three experts and scholars from education and psychology evaluated the content validity of the scale. Finally, we randomly selected 534 Chinese primary school teachers to form a sample, and then tested the reliability and validity of the scale by SPSS and Amos software. We found that the scale of Chinese primary school teachers’ job embeddedness consists of five domains, including 28 items. The reliability and validity of the scale is acceptable. The five domains were named “fit to organization”, “fit to community”, “organization-related sacrifice”, “links to community” and “fit to family”. Therefore, we come to the following conclusions: first, the structural model of job embeddedness of Chinese primary school teachers consists of five dimensions. Second, the scale gets a good reliability and validity.
“I’ll tell you later on”
Authors: Mlynář Jakub 
Published: March 2020

Abstract
This article investigates an interactional phenomenon in which oral history interview participants
deal with temporal structure in extended storytelling. It is based on the observation that while narrating a
life story, participants routinely use its temporal structure as an organizing principle of the interview.
Drawing inspiration from Sacks’ notion of tying devices and Genette’s distinction of prolepsis/analepsis, I
have identified two forms of practices that interrelate storytelling sequences in an interview. For the first
form, I propose the term analeptic tying: in this practice, turns produced earlier are treated as a resource
for the current turn. For the second form, I propose the term proleptic tying, which refers to planned turns
of speech that have yet to be produced being treated as a resource. I discuss the proleptic and analeptic
tying devices in relation to relevant research in ethnomethodology/conversation analysis, which is the
approach taken in this article.
Antioxidant Activity of Blended of Robusta Coffee (Coffea canephora L.) with The White Turmeric (Curcuma zedoaria (Berg.) Roscoe) and Wild Ginger (Curcuma xantorrhiza, Roxb.)
Authors: Gafar P A , Agustini S 
Published: March 2020

Abstract
This research aims to study the manufacture of herbal mixed coffee by blending the ground roasted coffee with the white turmeric extract and wild Javanese turmeric extract as a natural additive. The treatment was the ratio between ground roasted coffee and white turmeric and wild Javanese turmeric extract, which consisted of three levels namely 80:20%, 60:40%, 40:60%. The measurement of antioxidant activity was carried out using the DPPH method as % inhibition. The results showed that the antioxidant activity of coffee mixed with white turmeric was 18%, 19%, 48% respectively for each ratio and IC50 of 12.98%; while coffee mixed with Javanese turmeric extract showed the antioxidant activity of 20%; 32%; 48% with IC50 7.71%.
In-vivo Test of Calcium Soap from Palm Fatty Acid Distillate (PFAD) in Three Cow Farms
Authors: Indarto Antonius , Handojo Lienda A. , Shofinita Dian , Saadi Muhammad R. , Yulistia Dea , Hasyyati Fathinah I. 
Published: March 2020

Abstract
Palm Fatty Acid Distillate (PFAD) is a by-product of Crude Palm Oil (CPO) refinery. As the largest CPO producer, Indonesia exported an abundant amount of PFAD without further processing. The use of calcium soap as a supplement for ruminant feed will increase the productivity of dairy cows in Indonesia. Hence, this study reported in-vivo tests that were conducted to determine the effect of calcium soap supplementation on dairy cows. The result showed that feeding calcium soap to dairy cows could increase productivity and extend the lactation period. The milk quality also improved by the increase of fat content and density which lead to an increase in milk prices. Based on the field observations in three animal husbandries, calcium soap improved the health and fertility of cows.
Production Nanocellulose from Raw Materials For Oil Palm Empty Bunches (TKKS) with Hydrolysis and Freeze Drying Methods
Authors: Emmanuela Maria W , Kusumawati Endang , Regiana Anggi , Suminar Dian Ratna 
Published: March 2020

Abstract
Accumulation Oil Palm Empty Bunches (TKKS) cause environmental problems if not solved. TKKS has a high cellulose composition, so it can be used as nanocellulose which has a higher value. The process of making nanocellulose from TKKS through the isolation stage of α-cellulose with a delignification process using Sodium Hydroxide (NaOH) 17.5% (b/v) at 80°C, bleaching using Hydrogen Peroxide (H2O2) 10% (v/v), hydrolysis using sulfuric acid and drying using freeze drying at a heating temperature of 53°C for 7 hours. Cellulose yield produced from the isolation process of α-cellulose from TKKS was 20.8%. The results of drying process will reduce the aggregation of particles in the nanocellulose produced, so that nanocellulose is produced at 160-298 nm.
Optimization of Ceric Ammonium Nitrate and Ferrous Ammonium Sulfate in the Synthesis of Chitosan-Graft-Maleic Anhydride
Authors: Nadia Milla , Timotius Daniel , Wirawan Sang K. , Eviana Putri Nur Rofiqoh , Kusumastuti Yuni 
Published: March 2020

Abstract
Amongst various methods for chitosan modification, grafting process is one of the promising techniques to alter chitosan surface properties. Maleic anhydride (MA) was grafted onto chitosan in the presence of an initiator to produce chitosan-graft-maleic anhydride (CTS- graft-MA). In this study, we compared and optimized two types of initiators: Ceric Ammonium Nitrate (CAN) and Ferrous Ammonium Sulfate (FAS) with various ratios in the synthesis of chitosan-graft-maleic anhydride. Chitosan was dissolved in dilute acetic acid solution. Subsequently, the initiator was added into solution to initiate the reaction and maleic anhydride was added immediately afterward. The reaction was carried out for 45 minutes at 70 °C with 430 rpm of agitation. The results of Fourier Transformed Infrared (FTIR) spectroscopy showed an appearance of a new peak at 1705.07 cm−1 which belongs to C=O bands for both initiators. To find out the optimum results from CAN and FAS, the back-titration method was performed. The optimum ratio for CAN is 2.51% with the acidic content 5.4421 mmol/gr, while FAS is 0.268% with the acidic content 4.4673 mmol/gr. In conclusion, CAN is more effective than FAS to initiate the grafting process on CTS-graft- MA.
Electron correlation effects in isomers of C20
Authors: Cleland Deidre M , Fletcher Emily K , Kuperman Ariel , Per Manolo C 
Published: March 2020

AbstractThe C20molecule exists as three low-energy isomers: the monocyclic ring, a corannulene-like bowl structure, and the cage-the smallest possible fullerene. The curious structures of these isomers, along with the valuable properties and possible applications of fullerenes more generally, mean that C20has attracted interest both experimentally and computationally. Unfortunately, previous theoretical studies have found these C20isomers present a significant computational challenge, and widely used methods such as Density Functional Theory with different functionals have been unable to agree on even the relative ordering of the isomer energies. Even accurate high-level methods such as coupled-cluster with singles, doubles, and perturbative triples (CCSD(T)) and Diffusion Monte Carlo (DMC) have previously disagreed on the energetic ordering of these isomers. Here we re-examine the ring, bowl, and cage isomers of C20using a more accurate DMC technique. We employ a novel method to go beyond the single-determinant DMC approaches previously used, and instead use more accurate multi-determinant trial wavefunctions. Our results show that the fullerene cage exhibits different electron correlation effects than the ring and bowl, which when taken into account leads to a reordering of their relative energies. This finally establishes agreement between DMC and relatively recent complete-basis CCSD(T) results, thereby resolving a long-standing disparity between these two high-level descriptions of the C20isomers. The approach we use is generalisable, and could be used to provide insight into even larger systems in future.