bluuebunny/crossref_metadata_2025_split huggingface dataset:
Utility of published skinfold thickness equations for prediction of body composition in very young New Zealand children
Authors: Asadi Sharin , Bloomfield Frank H. , Alexander Tanith , McKinlay Chris J. D. , Rush Elaine C. , Harding Jane E. ,   for the hPOD, HUMBA and DIAMOND Study Teams
Published: April 2020

AbstractMeasurement of body composition is increasingly important in research and clinical settings but is difficult in very young children. Bioelectrical impedance analysis (BIA) and air displacement plethysmography (ADP) are well-established but require specialist equipment so are not always feasible. Our aim was to determine if anthropometry and skinfold thickness measurements can be used as a substitute for BIA or ADP for assessing body composition in very young New Zealand children. We used three multi-ethnic cohorts: 217 children at a mean age of 24·2 months with skinfold and BIA measurements; seventy-nine infants at a mean age of 20·9 weeks and seventy-three infants at a mean age of 16·2 weeks, both with skinfold and ADP measurements. We used Bland–Altman plots to compare fat and fat-free mass calculated using all potentially relevant equations with measurements using BIA or ADP. We also calculated the proportion of children in the same tertile for measured fat or fat-free mass and tertiles (i) calculated using each equation, (ii) each absolute skinfold, and (iii) sum of skinfold thicknesses. We found that even for the best equation for each cohort, the 95 % limits of agreement with standard measures were wide (25–200 % of the mean) and the proportion of children whose standard measures fell in the same tertile as the skinfold estimates was ≤69 %. We conclude that none of the available published skinfold thickness equations provides good prediction of body composition in multi-ethnic cohorts of very young New Zealand children with different birth history and growth patterns.
Integrative phenotypic and gene expression data identify myostatin as a muscle growth inhibitor in Chinese shrimp Fenneropenaeus chinensis
Authors: Kong Jie , Yan Yunjun , Lu Xia , Luan Sheng , Meng Xianhong , Dai Ping , Chen Baolong , Cao Baoxiang , Qiang Guangfeng , Luo Kun 
Published: April 2020

AbstractGrowth traits, largely determined by muscle growth, are the most critical economic traits in shrimp breeding. Myostatin (Mstn) is a conserved inhibitor of muscle growth in vertebrates, but until now solid evidence supporting a similar function of Mstn in invertebrates has been lacking. In the present study, we examined the Mstn expression along with growth trait data in a Fenneropenaeus chinensis population, to establish a potential correlation between Mstn and growth. The heritabilities of FcMstn expression, body weight at 190 days of culture, body weight and length at 230 days of culture, and average daily gain were estimated using 773 individuals and a thirteen-generation pedigree. The results showed FcMstn expression was negatively correlated with the growth traits, and the mean FcMstn expression in females was significantly lower than that of males, indicating Mstn negatively regulates muscle growth in shrimp, and its lower expression may underscore the faster growth of females. Low heritabilities were detected for FcMstn expression, suggesting that the expression of Mstn might be heritable in shrimp. These results provide strong support for a growth inhibitory function of Mstn in F. chinensis, and suggest a potential method for selective breeding of this species without substantial experimental resources and labor force.
Screening of Natural Products and Approved Oncology Drug Libraries for Activity against Clostridioides difficile
Authors: Pal Rusha , Seleem Mohamed N. 
Published: April 2020

AbstractClostridioides difficile is the most common cause of healthcare-associated diarrhea. Infection of the gastrointestinal tract with this Gram-positive, obligate anaerobe can lead to potentially life-threatening conditions in the antibiotic-treated populace. New therapeutics are urgently needed to treat this infection and prevent its recurrence. Here, we screened two libraries from the National Cancer Institute, namely, the natural product set III library (117 compounds) and the approved oncology drugs set V library (114 compounds), against C. difficile. In the two libraries screened, 17 compounds from the natural product set III library and 7 compounds from the approved oncology drugs set V library were found to exhibit anticlostridial activity. The most potent FDA-approved drugs (mitomycin C and mithramycin A) and a promising natural product (aureomycin) were further screened against 20 clinical isolates of C. difficile. The anticancer drugs, mitomycin C (MIC50 = 0.25 μg/ml) and mithramycin A (MIC50 = 0.015 μg/ml), and the naturally derived tetracycline derivative, aureomycin (MIC50 = 0.06 μg/ml), exhibited potent activity against C. difficile strains. Mithramycin A and aureomycin were further found to inhibit toxin production by this pathogen. Given their efficacy, these compounds can provide a quick supplement to current treatment to address the unmet needs in treating C. difficile infection and preventing its recurrence.
Modelling and observations: Comparison of the magnetic field properties in a prominence
Authors: Mackay D. H. , Schmieder B. , López Ariste A. , Su Y. 
Published: April 2020

Context. Direct magnetic field measurements in solar prominences occur infrequently and are difficult to make and interpret. As a consequence, alternative methods are needed to derive the main properties of the magnetic field that supports the prominence mass. This is important for our understanding of solar prominences, but also for understanding how eruptive prominences may affect space weather.
Aims. We present the first direct comparison of the magnetic field strength derived from spectro-polarimetric observations of a solar prominence, with corresponding results from a theoretical flux rope model constructed from on-disc normal component magnetograms.
Methods. We first used spectro-polarimetric observations of a prominence obtained with the magnetograph THEMIS operating in the Canary Islands to derive the magnetic field of the observed prominence by inverting the Stokes parameters measured in the He D3 line. Next, we constructed two data-constrained non-linear force-free field (NLFFF) models of the same prominence. In one model we assumed a strongly twisted flux rope solution, and in the other a weakly twisted flux rope solution.
Results. The physical extent of the prominence at the limb (height and length) is best reproduced with the strongly twisted flux rope solution. The line-of-sight average of the magnetic field for the strongly twisted solution results in a magnetic field that has a magnitude of within a factor of 1−2 of the observed magnetic field strength. For the peak field strength along the line of sight, an agreement to within 20% of the observations is obtained for the strongly twisted solution. The weakly twisted solution produces significantly lower magnetic field strengths and gives a poor agreement with the observations.
Conclusions. The results of this first comparison are promising. We found that the flux rope insertion method of producing a NLFFF is able to deduce the overall properties of the magnetic field in an observed prominence.
Optical and X-ray discovery of the changing-look AGN IRAS 23226-3843 showing extremely broad and double-peaked Balmer profiles
Authors: Kollatschny W. , Grupe D. , Parker M. L. , Ochmann M. W. , Schartel N. , Herwig E. , Komossa S. , Romero-Colmenero E. , Santos-Lleo M. 
Published: April 2020

Aims.We detected a very strong X-ray decline in the galaxy IRAS 23226-3843 within theXMM-Newtonslew survey in 2017. Subsequently, we carried out multi-band follow-up studies to investigate this fading galaxy in more detail.Methods.We took deep follow-upSwift,XMM-Newton, andNuSTARobservations in combination with optical SALT spectra of IRAS 23226-3843 in 2017. In addition, we reinspected optical, UV, and X-ray data that were taken in the past.Results.IRAS 23226-3843 decreased in X-rays by a factor of more than 30 with respect to ROSAT andSwiftdata taken 10 to 27 years before. The broadbandXMM-Newton/NuSTARspectrum is power-law dominated, with a contribution from photoionized emission from cold gas, likely the outer accretion disk or torus. The optical continuum decreased by 60% and the Balmer line intensities decreased by 50% between 1999 and 2017. The optical Seyfert spectral type changed simultaneously with the X-ray flux from a clear broad-line Seyfert 1 type in 1999 to a Seyfert 1.9 type in 2017. The Balmer line profiles in IRAS 23226-3843 are extremely broad. The profiles during the minimum state indicate that they originate in an accretion disk. The unusual flat Balmer decrement Hα/ Hβwith a value of 2 indicates a very high hydrogen density ofnH >  1011cm−3at the center of the accretion disk. IRAS 23226-3843 shows unusually strong FeII blends with respect to the broad line widths, in contrast to what is known from Eigenvector 1 studies.
Management of Wounds with Exposed Bones or Tendons in Children by Vacuum-Assisted Closure Therapy: A Prospective Study
Authors: Rasool Altaf , Bashir Sheikh Adil , Ahmad Prince Ajaz , Bijli Akram Hussain , Baba Umer Farooq , Yasir Mir , Wani Adil Hafeez 
Published: April 2020

Abstract
Background The management of complex soft tissue defects with exposed bones/tendons is always a challenging task for the surgeon and the problem becomes more pronounced when it comes to the management of these wounds in children. Though flap procedures are considered the standard for managing the complex soft tissue defects with exposed bones/tendons yet small blood vessels for anastomosis, long operative period, increased chances of perioperative thrombosis, and difficult perioperative management in children add to the difficulty in performing flap procedures in children. The vacuum-assisted closure (VAC) therapy has emerged as a novel modality for the management of the difficult wounds with added advantages, especially in children.
Objective To evaluate the efficacy of VAC in the management of wounds with exposed bones/tendons in children.
Patients and Method Forty-six children of complex wounds with exposed bones/tendons were included in the study from July 2016 to June 2018.
Results Out of 46 patients, 31 were male; the patients had a mean age of 8.4 years. Road traffic accident was the most common mode of injury (54%), with most of the wounds located over extremities. The mean duration of VAC therapy was 12 days. More than 90% coverage of the exposed structure was seen in 89% of patients. The wounds were definitively managed by split-thickness skin graft in 89% of patients and flap cover in 6.5% of patients. The mean cost of the VAC therapy at our government run hospital was 187 Indian rupees per day. No significant major complications were seen during the treatment.
Conclusion VAC therapy is an efficient, safe, and cost-effective modality of treatment for the management of complex wounds in the pediatric population.
Recent Advances in Decarboxylative Reactions of Alkynoic Acids
Authors: Lee Sunwoo , Idris Muhammad Aliyu 
Published: April 2020

Alkynoic acids have been widely employed as alkyne and alkene sources in decarboxylative reactions. Alkynoic acid coupling leads to the formation of direct coupling products and cyclized products through sequential reactions. Moreover, homocoupling and multicomponent reactions have been developed. The decarboxylative addition of alkynoic acids generates the corresponding alkene products. A number of synthetic methods are utilized for the preparation of arylpropynoic acids including the Sonogashira coupling and the carboxylation of terminal alkynes. Recently, the use of decarboxylative halogenations has also been reported. This review covers decarboxylative reactions of alkynoic acids reported between 2013 and 2019; further, it is divided into several sections according to the type of reaction.1	Introduction2	Direct Coupling3	Sequential Reactions4	Homocoupling5	Multicomponent Reactions6	Addition7	Halogenations8	Synthesis of Alkynoic Acids9	Conclusion
Methodological issues in the study of phonetic symbolism
Authors: Tsur Reuven , Gafni Chen 
Published: April 2020

Abstract
There is a growing research literature on phonetic symbolism in poetry, sometimes with incongruent results.
Through a theoretical structural analysis we show that, (a) individual speech sounds have (sometimes conflicting) potentials to
suggest elementary percepts, such as abruptness, hardness, smoothness; and (b) from these elementary percepts some general
psychological atmosphere may be abstracted that may be individuated in specific emotions as ‘love,’ ‘joy,’ or ‘anger,’ by semantic
feature-addition. This proposal can reconcile incongruous research results. Sound-symbolic lexical entries are governed by similar
principles, but fossilized. Large-scale statistical investigation may reveal significant sound-symbolic effects only when the same
phonemes repeat throughout the poem. They may, however, miss conspicuous local sound effects, revealed only by local analysis.
Some sceptical conclusions in the research literature may be due to this phenomenon. The proposed method may account not only for
statistical correlations, but also for the perception of a pervasive emotional atmosphere in a poem.
Mitochondrial DNA mutations induce mitochondrial biogenesis and increase the tumorigenic potential of Hodgkin and Reed–Sternberg cells
Authors: Haumann Sophie , Boix Julia , Knuever Jana , Bieling Angela , Vila Sanjurjo Anton , Elson Joanna L , Blakely Emma L , Taylor Robert W , Riet Nicole , Abken Hinrich , Kashkar Hamid , Hornig-Do Hue-Tran , Wiesner Rudolf J 
Published: April 2020

Abstract
Functioning mitochondria are crucial for cancer metabolism, but aerobic glycolysis is still considered to be an important pathway for energy production in many tumor cells. Here we show that two well established, classic Hodgkin lymphoma (cHL) cell lines harbor deleterious variants within mitochondrial DNA (mtDNA) and thus exhibit reduced steady-state levels of respiratory chain complexes. However, instead of resulting in the expected bioenergetic defect, these mtDNA variants evoke a retrograde signaling response that induces mitochondrial biogenesis and ultimately results in increased mitochondrial mass as well as function and enhances proliferation in vitro as well as tumor growth in mice in vivo. When complex I assembly was impaired by knockdown of one of its subunits, this led to further increased mitochondrial mass and function and, consequently, further accelerated tumor growth in vivo. In contrast, inhibition of mitochondrial respiration in vivo by the mitochondrial complex I inhibitor metformin efficiently slowed down growth. We conclude that, as a new mechanism, mildly deleterious mtDNA variants in cHL cancer cells cause an increase of mitochondrial mass and enhanced function as a compensatory effect using a retrograde signaling pathway, which provides an obvious advantage for tumor growth.
Left bundle branch pacing improved heart function in a 10-year-old child after a 3-month follow-up
Authors: Ji Wei , Chen Xueying , Shen Jie , Zhu Diqi , Chen Yiwei , Li Fen 
Published: April 2020

AbstractAimsAs a physiological pacing strategy, left bundle branch pacing (LBBP) were used to correct left bundle branch block (LBBB), however, there is no relevant report in children. We aimed to evaluate the feasibility of LBBP in children.Methods and resultsLeft bundle branch pacing was performed in a 10-year-old girl with a second-degree atrioventricular and LBBB. Under the guide of fluoroscopy, the pacing lead was deeply screwed into the interventricular septum to gain right bundle branch block (RBBB) pattern of paced QRS. Selective LBBP was achieved with a typical RBBB pattern of paced morphology and a discrete component between stimulus and ventricular activation in intracardiac electrogram and reached the standard of the stimulus to left ventricular activation time of 56 ms. At a 3-month follow-up, the LBBP acquired the reduction of left ventricular size and enhancement of left ventricular ejection fraction.ConclusionThe application of LBBP in a child was first achieved with inspiring preliminary results. The LBBP can be carried out in children by cautiousness under the premise of strict grasp of indications.
Canadian Noir: Consumer Culture, Colonial Nationalism and the Cardinal Series
Authors: Jones Manina 
Published: April 2020

Abstract
Giles Blunt’s Cardinal police-procedural novels and their recent television adaptations evidence the noir genre’s sombre aesthetic, focus on a morally tainted hero, are preoccupied with seemingly irrational violence, and fixate on unresolved past injustices. In doing so, they reflect Canada’s aesthetic and ethical relationship to questions of national and transnational culture, colonial territoriality, and the moral principles at stake in the representation of violence. This Canadian ‘re-branding’ of noir features is haunted by deep-seated historical dissension and the present-day repercussions that are at the heart of the country’s national identity. Focusing on the first season of Cardinal (2017) and the novel from which it was adapted, Forty Words for Sorrow (2002), this essay examines the series’ stylish – if conflicted – reworking of noir’s roots in American crime fiction and film, and its use of contemporary Nordic influences, which work to salvage a form of Canadian cultural authenticity from the cultural dominance of US television and film crime dramas.
Seeking support beyond alliance? Rethinking great power partner politics after the Cold War
Authors: Ding Lu , Sun Xuefeng 
Published: April 2020

Abstract
Since the end of the Cold War, establishing partnerships has been part and parcel of the grand strategy of great powers. The partners that great powers seek fall under the two categories of security partners and political-economic partners. Statistics show a significant variation in the proportions of great powers’ security partners. The authors argue that such variation is mainly determined by two factors, namely, great powers’ strategic threats, and their ways of maintaining national security [self-help or security-dependent (on the United States)]. Specifically, both the security-dependent great powers that are under China’s strategic threat and the self-help great powers that are under the US’s strategic threat have a higher proportion of security partners than the security-dependent great powers that are not under China’s strategic threat and the self-help great powers that are under China’s strategic threat. These findings will help to refine the current theories of great power politics.
Efficacy of dalbavancin against MRSA biofilms in a rat model of orthopaedic implant-associated infection
Authors: Silva Vanessa , Antão H Sofia , Guimarães João , Prada Justina , Pires Isabel , Martins Ângela , Maltez Luís , Pereira José E , Capelo José L , Igrejas Gilberto , Poeta Patrícia 
Published: April 2020

Abstract

Objectives
The aim of this study was to evaluate the efficacy of dalbavancin against MRSA biofilm-related infection in orthopaedic implants in vivo.

Methods
One MRSA strain isolated from human osteomyelitis was used to promote biofilm formation on the surface of screws. The implants were inserted in the proximal tibia under general anaesthesia. Thirty-nine Wistar rats were divided into three groups [control group (no treatment), Group 1 (7 days of treatment) and Group 2 (14 days of treatment)]; both treatment groups were administered dalbavancin intraperitoneally and euthanized after treatment. cfu of bacteria present in both the tibia and the implant were quantified. The infection severity was assessed by histopathology and scored from 0 (no infection) to 4 (severe infection).

Results
The high number of cfu/g and cfu/mL present in the control group indicated a well-established infection. There was a significant reduction in cfu in rats treated with dalbavancin both in the tibia (2.8 × 105 cfu/g) and the implant (1.1 × 106 cfu/mL) in Group 1 (1.8 × 103 cfu/g and 2.4 × 105 cfu/mL, respectively) and in Group 2 (8.2 cfu/g and 8.2 × 103 cfu/mL, respectively). Most animals from the control group presented an infection scored as 3 (severe). At the end of the experiment, most rats from Groups 1 and 2 presented an infection scored as 2 (moderate) and 0 (no infection), respectively.

Conclusions
Although there was a marked decrease in cfu number, signs of biofilm-induced infection prevailed after 14 days of treatment. Further studies should be carried out to evaluate the potential of dalbavancin in the treatment of bone and orthopaedic implant-associated MRSA infections.
Methyltransferase DnmA is responsible for genome-wide N6-methyladenosine modifications at non-palindromic recognition sites in Bacillus subtilis
Authors: Nye Taylor M , van Gijtenbeek Lieke A , Stevens Amanda G , Schroeder Jeremy W , Randall Justin R , Matthews Lindsay A , Simmons Lyle A 
Published: April 2020

AbstractThe genomes of organisms from all three domains of life harbor endogenous base modifications in the form of DNA methylation. In bacterial genomes, methylation occurs on adenosine and cytidine residues to include N6-methyladenine (m6A), 5-methylcytosine (m5C), and N4-methylcytosine (m4C). Bacterial DNA methylation has been well characterized in the context of restriction-modification (RM) systems, where methylation regulates DNA incision by the cognate restriction endonuclease. Relative to RM systems less is known about how m6A contributes to the epigenetic regulation of cellular functions in Gram-positive bacteria. Here, we characterize site-specific m6A modifications in the non-palindromic sequence GACGmAG within the genomes of Bacillus subtilis strains. We demonstrate that the yeeA gene is a methyltransferase responsible for the presence of m6A modifications. We show that methylation from YeeA does not function to limit DNA uptake during natural transformation. Instead, we identify a subset of promoters that contain the methylation consensus sequence and show that loss of methylation within promoter regions causes a decrease in reporter expression. Further, we identify a transcriptional repressor that preferentially binds an unmethylated promoter used in the reporter assays. With these results we suggest that m6A modifications in B. subtilis function to promote gene expression.
The consequences of altered microbiota in immune-related chronic kidney disease
Authors: Lau Wei Ling , Chang Yongen , Vaziri Nosratola D. 
Published: April 2020

Abstract
The normal gut microbiome modulates host enterocyte metabolism and shapes local and systemic immunity. Accumulation of urea and other waste products in chronic kidney disease induces gut dysbiosis and intestinal wall inflammation (leaky gut). There are decreased numbers of bacteria that generate short-chain fatty acids, which are an important nutrient source for host enterocytes and also contribute to regulation of the host immune system. Anaerobic proteolytic bacteria that express urease, uricase and indole and p-cresol enzymes, such as Enterobacteria and Enterococci, are increased. Microbial-derived uremic toxins such as indoxyl sulfate and trimethylamine N-oxide contribute to the pathophysiology of immune-related kidney diseases such as diabetic nephropathy, lupus nephritis and immunoglobulin A (IgA) nephropathy. Animal and clinical studies suggest potential benefits of dietary and probiotic interventions in slowing the progression of immune-related kidney diseases.