bluuebunny/crossref_metadata_2025_split huggingface dataset:
Self‐Assembling Proteins for Design of Anticancer Nanodrugs
Authors: Zou Qianli , Chang Rui , Yan Xuehai 
Published: March 2020

AbstractInspired by the diverse protein‐based structures and materials in organisms, proteins have been expected as promising biological components for constructing nanomaterials toward various applications. In numerous studies protein‐based nanomaterials have been constructed with the merits of abundant bioactivity and good biocompatibility. However, self‐assembly of proteins as a dominant approach in constructing anticancer nanodrugs has not been reviewed. Here, we provide a comprehensive account of the role of protein self‐assembly in fabrication, regulation, and application of anticancer nanodrugs. The supramolecular strategies, building blocks, and molecular interactions of protein self‐assembly as well as the properties, functions, and applications of the resulting nanodrugs are discussed. The applications in chemotherapy, radiotherapy, photodynamic therapy, photothermal therapy, gene therapy, and combination therapy are included. Especially, manipulation of molecular interactions for realizing cancer‐specific response and cancer theranostics are emphasized. By expounding the impact of molecular interactions on therapeutic activity, rational design of highly efficient protein‐based nanodrugs for precision anticancer therapy can be envisioned. Also, the challenges and perspectives in constructing nanodrugs based on protein self‐assembly are presented to advance clinical translation of protein‐based nanodrugs and next‐generation nanomedicine.
Hochfester Aufbeton zur Tragwerksverstärkung
Authors: Randl Norbert , Steiner Martin 
Published: March 2020

AbstractIn Bauteil‐ und Kleinkörperversuchen mit unterschiedlichen Prüfkörperformen und Test‐Setups wurde das Verbundtragverhalten zwischen hochfestem Auf‐ und normalfestem Altbeton untersucht. Während in Teil 1 über die Kleinkörperversuche berichtet wurde, stehen im Teil 2 Versuche an plattenartigen Bauteilen mit Fokus auf den flächigen Verbund im Vordergrund. Neben den herkömmlich betonierten normal‐ und höherfesten Aufbetonschichten wurde an zwei Bauteilen die Aufbetonage unter zyklischer Anregung der Grundplatte durchgeführt. Insgesamt bestätigten auch die Bauteilversuche die schon am Kleinkörper beobachtete Verbesserung der Verbundeigenschaften mit hochfestem Aufbeton. Sämtliche Platten mit normalfestem Aufbeton wiesen im Bereich der Verbundfugen im Bruchversuch lokale Schädigungen auf, bei den mit hochfestem Beton verstärkten Platten hingegen waren bis auf jene mit zyklischer Anregung während der Betonage kaum Verbundbeeinträchtigungen erkennbar.
Clinicopathological features and oncological outcomes of patients with young-onset rectal cancer
Authors: Zaborowski A M , Murphy B , Creavin B , Rogers A C , Kennelly R , Hanly A , Martin S T , O'Connell P R , Sheahan K , Winter D C 
Published: March 2020

Abstract

Background
The incidence of rectal cancer among adults aged less than 50 years is rising. Survival data are limited and conflicting, and the oncological benefit of standard neoadjuvant and adjuvant therapies is unclear.

Methods
Disease-specific outcomes of patients diagnosed with rectal cancer undergoing surgical resection with curative intent between 2006 and 2016 were analysed.

Results
A total of 797 patients with rectal cancer were identified, of whom 685 had surgery with curative intent. Seventy patients were younger than 50 years and 615 were aged 50 years or more. Clinical stage did not differ between the two age groups. Patients aged less than 50 years were more likely to have microsatellite instability (9 versus 1·6 per cent; P = 0·003) and Lynch syndrome (7 versus 0 per cent; P &lt; 0·001). Younger patients were also more likely to receive neoadjuvant chemoradiotherapy (67 versus 53·3 per cent; P = 0·003) and adjuvant chemotherapy (41 versus 24·2 per cent; P = 0·006). Five-year overall survival was better in those under 50 years old (80 versus 72 per cent; P = 0·013). The 5-year disease-free survival rate was 81 per cent in both age groups (P = 0·711). There were no significant differences in the development of locoregional recurrence or distant metastases.

Conclusion
Despite accessing more treatment, young patients have disease-specific outcomes comparable to those of their older counterparts.
Pains and gains of environmental management system certification for the sustainable development of manufacturing companies: Heterogeneous effects of industry peer learning
Authors: Wang Jing , Mao Yunshi 
Published: March 2020

AbstractEnvironmental sustainability is a growing global concern. Environmental management systems (EMS) could be an effective strategic tool to help firms deal with their sustainable development. However, whether EMS certification pays off financially and how it takes effect can be debated. Thus far, these questions remain largely under‐researched. In particular, the effects of EMS certification on financial performance are inconclusive, and the reasons explaining the effects are underdeveloped. This study aims to enrich the current research by exploring the mediating and moderating roles from the perspective of cost‐efficiency trade‐offs to reveal how EMS certification affects financial performance. Applying a PROCESS procedure analysis and causal mediation analysis to a sample of 1,751 Chinese listed manufacturing firms from 2008 to 2016, this study shows that the effect of EMS certification on firms' financial performance is insignificant because their operating costs burden increases while their marketing efficiency and managerial efficiency improve. For the first time, this study demonstrates the moderating role of industry peer learning, as the mediating effects decrease with the growth of industry peer learning.
Predicting the effect of 5‐fluorouracil–based adjuvant chemotherapy on colorectal cancer recurrence: A model using gene expression profiles
Authors: Chen Quan , Gao Peng , Song Yongxi , Huang Xuanzhang , Xiao Qiong , Chen Xiaowan , Lv Xinger , Wang Zhenning 
Published: March 2020

ABSTRACTIt is critical to identify patients with stage II and III colorectal cancer (CRC) who will benefit from adjuvant chemotherapy (ACT) after curative surgery, while the only use of clinical factors is insufficient to predict this beneficial effect. In this study, we performed genetic algorithm (GA) to select ACT candidate genes, and built a predictive model of support vector machine (SVM) using gene expression profiles from the Gene Expression Omnibus database. The model contained four ACT candidate genes (EDEM1, MVD, SEMA5B, and WWP2) and TNM stage (stage II or III). After using Subpopulation Treatment Effect Pattern Plot to determine the optimal cutoff value of predictive scores, the validated patients from The Cancer Genome Atlas database can be divided into the predictive ACT‐benefit/‐futile groups. Patients in the predictive ACT‐benefit group with 5‐fluorouracil (5‐Fu)–based ACT had significantly longer relapse‐free survival (RFS) compared to those without ACT (P = .015); However, the difference in RFS in the predictive ACT‐futile group was insignificant (P = .596). The multivariable analysis found that the predictive groups were significantly associated with the effect of ACT (Pinteraction = .011). Consequently, we developed a predictive model based on the SVM and GA algorithm which was further validated to define patients who benefit from ACT on recurrence.
Synthesis and Biological Evaluation of Novel Carbazole Hybrids as Promising Antimicrobial Agents
Authors: Shaikh Mahamadhanif S. , Chandrasekaran Balakumar , Palkar Mahesh B. , Kanhed Ashish M. , Kajee Afsana , Mlisana Koleka P. , Singh Parvesh , Ghai Meenu , Cleopus Mahlalela Mavela , Karpoormath Rajshekhar 
Published: March 2020

AbstractTwo series of carbazole analogs of 8‐methoxy‐N‐substituted‐9H‐carbazole‐3‐carboxamides (series 1) and carbazolyl substituted rhodanines (series 2) were synthesized through facile synthetic routes. All the final compounds from these two series were evaluated for their preliminary in vitro antifungal and antibacterial activity against four fungal (Candida albicans, Cryptococcus neoformans, Cryptococcus tropicalis and Aspergillus niger) and four bacterial (Staphylococcus aureus, Bacillus subtilis, Escherichia coli and Pseudomonas aeruginosa) strains, respectively. Among the tested compounds, three compounds of series 1 displayed promising antifungal and antibacterial activity, especially against C. neoformans and S. aureus. In addition, one compound of series 1 displayed notable antimicrobial activity (MIC: 6.25 μg/mL) against clinical isolates of C. albicans and C. neoformans (MIC: 12.5 μg/mL). From the second series, four compounds exhibited significant antifungal and antibacterial activity, especially against C. neoformans and S. aureus. The most active compound of series 2 displayed a prominent antimicrobial activity against C. neoformans (MIC: 3.125 μg/mL) and S. aureus (MIC: 1.56 μg/mL), respectively.
Base Induced Condensation of Malononitrile with Erlenmeyer Azlactones: An Unexpected Synthesis of Multi‐Substituted Δ2‐Pyrrolines and Their Cytotoxicity
Authors: Anil Seegehalli M. , Sudhanva Muddenahalli S. , Swaroop Toreshettahally R. , Vinayaka Ajjampura C. , Rajeev Narasimhamurthy , Kiran Kuppalli R. , Shobith Rangappa , Sadashiva Maralinganadoddi P. 
Published: March 2020

AbstractAn efficient, metal free approach to synthesize multi‐substituted Δ2‐pyrroline derivatives by mild base catalyzed cyclocondensation of malononitrile with Erlenmeyer azlactones via 1,2 addition was developed. The modularity of this reaction was used to assemble a range of poly‐substituted pyrrolines. Further, synthesized products were screened for cytotoxic properties on different cancer cell lines such as A549 (Human lung adenocarcinoma cells), HeLa (Human cervical adenocarcinoma cells), Jurkat (Human chronic myeloid leukemia cells) and K562 (Human leukemic T cell Lymphoblast cells). Among the synthesized library of compounds, 6f and 6q displayed potent cytotoxic activity.
Novel use of fused cardiac computed tomography and transesophageal echocardiography for left atrial appendage closure
Authors: Peters Andrew , Motiwala Afaq , O'Neill Brian , Patil Pravin 
Published: March 2020

AbstractThe use of the Watchman left atrial appendage occlusion device (Boston Scientific Inc.) is becoming increasingly frequent in patients with atrial fibrillation. Cardiac computed tomography (CT) for device sizing pre‐procedure can help facilitate more accurate device selection compared with transesophageal echo (TEE) alone. CT can also help identify minor lobes and trabeculations that may not be apparent on TEE. We report a series of three cases to highlight the utility of a novel application of CT‐TEE fusion imaging to provide procedural guidance during Watchman implant and to assess for peri‐device leak post‐implant.
The impact of the extent of side branch disease on outcomes following bifurcation stenting
Authors: Zimarino Marco , Barbato Emanuele , Nakamura Sunao , Radico Francesco , Di Nicola Marta , Briguori Carlo , Gil Robert J. , Kanic Vojko , Perfetti Matteo , Pellicano Mariano , Mairic Kristina , Stankovic Goran ,   the European Bifurcation Club
Published: March 2020

AbstractObjectivesTo define the impact of side branch (SB) lesion length on clinical outcomes after percutaneous coronary intervention (PCI) on bifurcation lesions.BackgroundThe role of the SB lesion length remains questionable in PCI planning and its implication on clinical outcome is controversial.MethodsData from the retrospective multicenter EBC‐P2BiTO registry were analyzed. The primary endpoint was the occurrence of major adverse cardiac events (MACE), defined as the composite of cardiac death, myocardial infarction excluding periprocedural, or stent thrombosis at 13 months median follow‐up (IQR 11–28). By using propensity scores for inverse probability of treatment weighting (IPTW), the comparison of treatment groups was adjusted to correct for potential confounding.ResultsAmong 1,252 patients, SB was normal in 489 (39%), diseased in 763 (61%) cases. MACE occurred in 68 patients (5.4%). The optimal discriminant SB lesion length for MACE was ≥10 mm, with an area under the curve of 0.71 (p < .01).The incidence of MACE was higher among patients with SB lesions ≥10 mm (8%) than with normal SB (4.1%) (hazard ratio [HR], 2.8; 95% confidence interval [CI], 1.5–5.3; p = .001, IPTW‐adjusted) or SB lesions <10 mm (5.1%) (HR, 1.5; 95% CI, 1.1–3.3; p = .048, IPTW‐adjusted), being similar between these last two groups.ConclusionsIn bifurcation PCI, SB lesion length ≥ 10 mm identifies patients at higher risk of MACE than those with <10 mm SB lesions and those without SB disease, considering that no differences were observed among these last two groups. Careful planning is mandatory when approaching bifurcations with long SB lesions.
BRIDGE – ORANGE HEADQUARTERS – EAST AND WEST BRIDGES
Authors: Lopes Adriano , Gonçalves Sílvio , Breda Ricardo 
Published: March 2020

AbstractAt the top of Bridge building, the architect designed two crossings between opposing structural blocks, creating unique and privileged workspaces. They form the main structure of the various levels of roofs and give support to gardens, leisure areas and large glazed skylights. They are independent structural pieces supported by the building structure. This article addresses the solutions used as well as the cantilever construction method that has been implemented given the central and elevated location on site.
BUILDING AT ESTRELA 24 (LISBON) ‐ INNOVATION IN CONSTRUCTION OF THE REHABILITATION OF EXISTING OLD BUILDING (FEASABILITY PROCESS AND PHASES)
Authors: Pires Miguel M. 
Published: March 2020

AbstractThe following article describes a set of innovative solutions within the scope of a building rehabilitation contract located at Calçada da Estrela, N24 – Lisbon. Steel and composite structures were used to design bold solutions, best reconciling the construction stages and definitive phases with advantages in terms of feasibility, reduction of time and optimization of quantities, costs and waste. The use of BIM methodologies proved to be useful in clear understanding of the constructive phasing at the work site. Methodologies of structural calculation, constructive phases and monitoring of existing wall on‐site are presented.
PENHA DA BARROCA RAILWAY BRIDGE – CONTEMPORANY AESTHETICS, IMPROVED PERFORMANCE
Authors: Afecto Ricardo , Lopes Nuno 
Published: March 2020

AbstractThe Penha da Barroca Bridge is a railway bridge that is under construction and is within the framework of the Beira Baixa railway line modernization project which itself is a key part of the Atlantic Corridor that concerns Portugal, Spain, France and Germany. All foreseen interventions in this railway line were also identified as priority investments by the National Stategic Plan for Transports and Infrastructures – time‐horizon 2014‐2020 (PETI3+). The bridge is closely to Guarda and is composed by steel latticed columns supporting a steel‐concrete composite deck. The steel component part of deck corresponds to a steel truss and the concrete component part is guaranteed by a concrete slab over which will be settled the rail track (ballasted track).
THE 125 MODULAR BRIDGES PROJECT TO PERU
Authors: André António , Ferraz Inês , Coelho Hugo , Fernandes José , Pacheco Pedro 
Published: March 2020

AbstractThis paper presents the general aspects that were considered on the design phase of 125 modular steel bridges developed in Portugal for delivery to a department of the Peruvian Ministry of Transport and Communications. Some of the main features of manufacturing, pre‐assembly and containerization in Turkey are also presented. Some of the tests performed on the floor modules are also introduced, namely the load tests until failure, the fatigue tests, the durability validation tests of the hot deep galvanized and the antiskid coating. The main results of the tests are also discussed, and their conclusions presented.
FIELD EVALUATION OF UNINTENDED COMPOSITE ACTION BETWEEN STEEL PLATE GIRDERS AND CONCRETE SLAB IN HIGHWAY BRIDGES
Authors: Lu Renxiang , Judd Johnn P. 
Published: March 2020

AbstractUnintended composite action between steel plate girders and concrete slab is a well‐recognized, but challenging aspect of evaluating highway bridge behaviour because the degree of slip between the slab and girder is difficult to predict. In this study, a method to evaluate the effect of unintended composite action using field testing is presented. The method is illustrated using a four‐span five girder highway bridge instrumented with strain gages at maximum positive and negative moment locations and subjected to a vehicle with a calibrated load. Results indicate that all the girders exhibited partial composite action. Measurement of composite action was less reliable when the instrumented location was not close to the load.
SHEAR STRENGTH OF STEEL‐CONCRETE COMPOSITE PLATE GIRDERS – A REVIEW
Authors: Nascimento Sérgio , Pedro José Oliveira 
Published: March 2020

ResumeThe shear strength of slender steel‐concrete composite plate girders is usually evaluated through one of the several design methods proposed in the last 50 years, taking into consideration the steel girders shear strength only. Although for composite girders the concrete slab may increase the shear strength, this contribution is not considered in these methods. But, the shear strength of steel‐concrete composite plate girders can be assessed by means of two recent proposed methods. The results from design methods are compared with experimental tests data.
BEHAVIOUR OF AUSTENITIC STAINLESS STEEL ANGLES UNDER CENTERED COMPRESSION
Authors: Sarquis Fernando R. , de Lima Luciano R. O. , da S. Vellasco Pedro C. G. , Rodrigues Monique C. , da Silva André T. 
Published: March 2020

AbstractThis paper presents an experimental and numerical analysis of 304 austenitic stainless steel hot‐rolled equal leg angles with fixed end supports. The experimental programme consisted of material characterisation under tension and compression tests, initial geometrical imperfections measurements and full‐scale tests to obtain ultimate loads and associated failure modes . Besides the experimental programme, a finite element numerical model was also developed to expand the studied sections and lengths through a parametric study to verify the accuracy of Eurocode 3 – Part 1.4 buckling curve.
Porous Frameworks Based on Supramolecular Ball Joints: Bringing Flexibility to Ordered 3D Lattices
Authors: Savastano Matteo , Bazzicalupi Carla , Bianchi Antonio 
Published: March 2020

AbstractXOFs‐type materials (X=M, C, S, that is, metal–organic frameworks, covalent organic frameworks and supramolecular organic frameworks, respectively) share a common unifying feature: mutual spatial orientation of constituting components is strictly directional and unchanging by design. Herein, we illustrate an alternate design for porous architectures, as rigid joints constituted by coordinative (MOFs), covalent (COFs), or hydrogen‐donor/acceptor (SOFs) bonds, are replaced by supramolecular ball joints, which confer unprecedented flexibility, especially angular, to porous networks. The obtained frameworks remain highly organized but are also permutable: lacking a forced convergence towards an immutable minimum energy structure, these systems remain able to adjust depending on external conditions. Results of POF (permutable organized framework) synthesis is a family of structures rather than a single pre‐determined three‐dimensional arrangement, as we demonstrate with an illustrative set of 5 XRD structures.
Euler‐Lagrange‐Modell zur Simulation des Carbonate‐Looping‐Prozesses
Authors: May Jan , Alobaid Falah , Stroh Alexander , Daikeler Alexander , Ströhle Jochen , Epple Bernd 
Published: March 2020

AbstractEs existieren bereits umfangreiche experimentelle Untersuchungen zum Carbonate‐Looping‐Prozess (CaL) in Bezug auf Prozessdesign und ‐entwicklung. Die wenigen numerischen Arbeiten beruhen hauptsächlich auf Lösung der 1D‐Bilanzgleichungen oder der Bestimmung von Gleichgewichtszuständen. Für eine genaue Prozessbeschreibung sind jedoch anspruchsvollere Ansätze erforderlich. Daher wurde ein dreidimensionales, transientes CFD‐DEM‐Modell für die Simulation im MWth‐Maßstab entwickelt. Zur Modellentwicklung und Validierung wurden experimentelle Daten einer 1‐MWth‐Pilotanlage, einem maßstabsverkleinerten kalten Modell und einer Quasi‐2D‐Laborwirbelschicht verwendet. Die numerischen Ergebnisse stimmen gut mit den Messungen überein und zeigen die Eignung des Modells.
Recent Highlights in Anti‐infective Medicinal Chemistry from South Africa
Authors: Veale Clinton G. L. , Müller Ronel 
Published: March 2020

AbstractGlobal advancements in biological technologies have vastly increased the variety of and accessibility to bioassay platforms, while simultaneously improving our understanding of druggable chemical space. In the South African context, this has resulted in a rapid expansion in the number of medicinal chemistry programmes currently operating, particularly on university campuses. Furthermore, the modern medicinal chemist has the advantage of being able to incorporate data from numerous related disciplines into the medicinal chemistry process, allowing for informed molecular design to play a far greater role than previously possible. Accordingly, this review focusses on recent highlights in drug‐discovery programmes, in which South African medicinal chemistry groups have played a substantive role in the design and optimisation of biologically active compounds which contribute to the search for promising agents for infectious disease.
Somatic Liver Knockout (SLiK): A Quick and Efficient Way to Generate Liver‐Specific Knockout Mice Using Multiplex CRISPR/Cas9 Gene Editing
Authors: Johnson Collin G. , Chen Tong , Furey Nika , Hemmingsen Madeline G. , Bissig Karl‐Dimiter 
Published: March 2020

AbstractSomatic liver knockout (SLiK) is a method developed to rapidly generate a liver‐specific knockout of one or several genes. This technique combines the strengths of CRISPR/Cas9 gene editing and hydrodynamic tail‐vein injection, a simple in vivo method for transfection of hepatocytes, to harness the powerful selection pressure of tyrosinemic livers to replace host hepatocytes with any desired gene deletion. In this protocol, we will describe sgRNA design and cloning, hydrodynamic tail‐vein injection of targeting constructs, and screening and validation methods for efficient in vivo gene editing. © 2020 by John Wiley & Sons, Inc.Support Protocol 1: sgRNA designSupport Protocol 2: sgRNA construction: daisy chaining multiple sgRNAsBasic Protocol: Delivery of DNA by hydrodynamic tail‐vein injection and liver repopulation of edited hepatocytesSupport Protocol 3: Validation of CRISPR/Cas9 cutting in vivo
Aseptic Technique
Authors: Bykowski Tomasz , Stevenson Brian 
Published: March 2020

AbstractThis article describes common laboratory procedures that can reduce the risk of culture contamination (sepsis), collectively referred as “aseptic technique.” Two major strategies for aseptic work are described: using a Bunsen burner and using a laminar flow hood. Both methods are presented in the form of general protocols applicable to a variety of laboratory tasks such as pipetting and dispensing aliquots, preparing growth media, and inoculating, passaging, and spreading microorganisms on petri dishes. © 2020 by John Wiley & Sons, Inc.