bluuebunny/crossref_metadata_2025_split huggingface dataset:
The behavioural and neuropathologic sexual dimorphism and absence of MIP-3α in tau P301S mouse model of Alzheimer’s disease
Authors: Sun Yao , Guo Yongqing , Feng Xuejian , Jia Meng , Ai Ning , Dong Yue , Zheng Yayuan , Fu Lu , Yu Bin , Zhang Haihong , Wu Jiaxin , Yu Xianghui , Wu Hui , Kong Wei 
Published: January 2020

Abstract
Background : Tau hyper-phosphorylation has been considered a major contributor to neurodegeneration in Alzheimer’s disease (AD) and related tauopathies, and has gained prominence in therapeutic development for AD. To elucidate the pathogenic mechanisms underlying AD and evaluate therapeutic approaches targeting tau, numerous transgenic mouse models that recapitulate critical AD-like pathology have been developed. Tau P301S transgenic mice is one of the most widely used mouse models in AD research. Extensive studies have demonstrated that sex significantly influences AD pathology, behavioral status and therapeutic outcomes, suggesting that studies using mouse models of AD must consider sex- and age-related differences in neuropathology, behavior, and plasma content. Method : We systematically investigated differences in tau P301S transgenic mice (PS19 line) and wildtype littermates of different sex behavioral performance, tau neuropathology and biomarkers in plasma and brain. Results : Male P301S transgenic mice exhibited significant changes in weight loss, survival rate, clasping, kyphosis, composite phenotype assessment, nest building performance, tau phosphorylation at Ser202/Thr205 and astrocyte activation compared to that of wild type littermates. In contrast, female P301S transgenic mice were only sensitive in the Morris Water Maze and open field test. In addition, we characterized the absence of macrophage-inflammatory protein (MIP-3α) and the upregulation of interferon (IFN)-γ, interleukin (IL)-5, and IL-6 in the plasma of P301S transgenic mice, which can be served as potential plasma biomarkers in P301S Tg mice. Male P301S transgenic mice expressed more monokine induced by IFN-γ (MIG), tumor necrosis factor-α (TNF-α), IL-10 and IL-13 than those of female P301S mice. Conclusion : Our findings highlight sexual dimorphism in the behavior, neuropathology, and plasma proteins in tau P301S transgenic AD mice, indicating that the use of male P301S transgenic mice may be more suitable for assessing anti-phosphorylated tau therapeutic strategies for AD and related tauopathies, and the MIP-3α may be a new potential plasma biomarker.
Vaccination accelerates hepatic erythroblastosis induced by blood-stage malaria
Authors: Delic Denis , Wunderlich Frank , Al-Quraishy Saleh , Abdel-Baki Abdel-Azeem S. , Dkhil Mohamed A. , Araúzo-Bravo Marcos J. 
Published: January 2020

Abstract
Background Vaccination induces survival of otherwise lethal blood-stage infections of the experimental malaria Plasmodium chabaudi . Blood-stage malaria induces extramedullary erythropoiesis in the liver. This study investigates how vaccination affects the course of malaria-induced expression of erythrocytic genes in the liver. Methods Female Balb/c mice were vaccinated at week 3 and week 1 before challenging with 10 6 P. chabaudi- parasitized erythrocytes. The non-infectious vaccine consisted of erythrocyte ghosts isolated from P. chabaudi -infected erythrocytes. Gene expression microarrays and quantitative real-time PCR were used to compare mRNA expression of different erythrocytic genes in the liver of vaccination-protected and non-protected mice during infections on days 0, 1, 4, 8, and 11 p.i. . Results Global transcriptomics analyses reveal vaccination-induced modifications of malaria-induced increases in hepatic gene expression on days 4 and 11 p.i.. On these days, vaccination also alters hepatic expression of the erythropoiesis-involved genes Ermap, Kel, Rhd , Rhag , Slc4a1, Gypa, Add2, Ank1, Epb4.1, Epb4.2, Epb4.9, Spta1, Sptb, Tmod1 , Ahsp, Acyp1 , Gata1, Gfi1b, Tal1, Klf1, Epor , and Cldn13 . In vaccination-protected mice, expression of these genes, except Epb4.1 , is significantly higher on day 4 p.i. than in un-protected non-vaccinated mice, reaches maximal expression at peak parasitaemia on day 8 p.i., and is slowed down or even decreased towards the end of crisis phase on day 11 p.i.. After day 1 p.i., Epor expression takes about the same course as that of the other erythroid genes. Hepatic expression of Epo, however, is delayed in both vaccinated and non-vaccinated mice for the first 4 days p.i. and is maximal at significantly higher levels in vaccinated mice on day 8 p.i. , before declining towards the end of crisis phase on day 11 p.i. . Conclusion The present data indicate that vaccination accelerates malaria-induced erythroblastosis in the liver for 1-2 days. This may contribute to earlier replenishment of peripheral red blood cells by liver-derived reticulocytes, which may favour final survival of otherwise lethal blood-stage malaria, since reticulocytes are not preferred as host cells by P. chabaudi .
Lessons learned, challenges and outlooks for decision-making after a decade of experience monitoring the impact of Indoor Residual Spraying in Benin, West Africa
Authors: Akogbéto Martin C , Dagnon Fortuné , Aikpon Rock , Osse Razaki , Salako Albert , Ahogni Idelphonse , Akinro Bruno , Sominahouin André , Sidick Aboubakar , Tokponnon Filémon , Padonou Germain Gil 
Published: January 2020

Abstract
Background Since 2008, Indoor Residual Spraying (IRS) has been performed in Benin in 19 districts, including 4 in southern Benin, 9 in Atacora, and 8 in Atacora, Alibori and Donga in northern Benin. However, Benin still struggles with questions about IRS cost benefit and epidemiological impact. We discussed lessons learned and challenges from 10 years of IRS in Benin to be shared with the stakeholders involved in vector control implementation for decisions making. Methods We have assessed entomological parameters in IRS communes since 2008. In all IRS intervention communes, decreases in Human Biting Rate (HBR) of An. gambiae, blood feeding inhibition and Entomological Inoculation Rate (EIR) as compared to control district have been measured. Results EIR was reduced by 80-90%, which is encouraging, but should be observed with caution because: (i) the reduction may be insufficient to decrease epidemiological indicators given that the residual EIR in IRS districts is still higher than it is in some regions of stable malaria; (ii) the reduction in EIR is based on comparisons with control communes, but it is difficult to select control areas with the same environmental characteristics as intervention areas; (iii) despite the reduction, half of all mosquitoes that entered IRS-treated houses succeeded in taking human blood meals. Further, there are behaviors among Benin’s population that limit IRS efficacy, including recent data showing that >90% of people are not protected by IRS between 7-10 PM. This is due to the fact that they remain outside and that most people are not protected from mosquito bites after 10 PM because they either sleep outside without IRS protection or inside without an ITN. Moreover, people have large amounts of clothing hanging on walls where mosquitoes can rest instead of IRS-treated walls. Finally, other components are important to consider in implementing IRS among which: (i) Vector resistance management strategies are sometimes poorly understood; this is actually different from should be replacing one insecticide with another after the emergence of resistance; (ii) African countries should prepare to finance IRS themselves. Conclusion To curtail residual malaria transmission, additional interventions able to target vectors escaping IRS should be prioritized.
Correlation between the efficacy of stem cell therapy for osteonecrosis of the femoral head and cell viability
Authors: Wu ZhanYu , Sun Qi , Liu Ming , Grottkau Brian , He ZhiXu , Zou Qiang , Ye Chuan 
Published: January 2020

Abstract
Background: Osteonecrosis of the femoral head (ONFH) is a common disease that greatly affects the quality of life of patients. Repair of the necrotic area is key to successful treatment. Currently, the combination of stem cell transplantation and decompression is used clinically to promote the repair of necrotic areas based on the characteristics of stem cells. However, a considerable number of patients do not achieve a satisfactory outcome in terms of repair of the femoral head necrotic area, and it is very important to determine the reasons for the poor curative effect. The aim of this study was to investigate the correlation between stem cell viability and the repair efficacy of stem cell therapy combined with core decompression for early-stage ONFH.
Methods: A total of 30 patients with idiopathic ONFH underwent core decompression combined with autologous stem cell transplantation. The Harris hip score (HHS) and difference in necrosis area before and after surgery were measured. The mean repair ratio was set as the threshold to divide the patients into group A (ratio above the mean) and group B (ratio below the mean). The ultrastructure, proliferative capacity, and multidirectional differentiation ability were compared between the groups.
Results : At 9 months after surgery, the HHS and magnetic resonance imaging (MRI) findings improved by varying degrees. Based on the mean repair ratio of (62.2 ± 27.0)%, the threshold for dividing the patients into groups A and B was set to 62.2%. Better repair (group A) was associated with more rapid proliferation and a healthier ultrastructure. The cells in group A showed stronger specific staining signifying osteogenic and chondrogenic differentiation; alkaline phosphatase (ALP) activity, an indicator of osteogenic differentiation, was higher in group A than in group B (OD, 2.39 ± 0.44 and 1.85 ± 0.52; p < 0.05).
Conclusions: The quality of implanted stem cells is closely related to treatment efficacy and determines whether the defective self-repair in the necrotic area can be corrected to enhance repair and thus achieve the desired therapeutic outcome.
A novel association of CCDC80 with gestational diabetes mellitus in pregnant women: a propensity score analysis from a case-control study
Authors: Liu Lei , Hu Jiajin , Wang Ningning , Liu Yang , Wei Xiaotong , Gao Ming , Ma Yanan , Wen Deliang 
Published: January 2020

Abstract
Background: Gestational diabetes mellitus (GDM) is a growing global epidemic. Our study aims to confirm the association between circulatory coiled-coil domain-containing 80 (CCDC80) in pregnant women with GDM, to investigate the discriminatory power of CCDC80 on GDM, and to explore the relationships between this molecular level and clinical cardiometabolic parameters. Methods: A 1:2 matched case-control study with 61 GDM patients and 122 controls was conducted using a propensity score matching protocol. All participants were screened from a multicenter prospective pre-birth cohort: Born in Shenyang Cohort Study (BISCS). During 24 and 28 weeks of gestation, follow-up individuals underwent an oral glucose tolerance test (OGTT) and blood sampling for cardiometabolic characterization. Results: Following propensity score matching adjustment for clinical variables, including maternal age, gestational age, body mass index, SBP and DBP, plasma CCDC80 levels were significantly decreased in patients with GDM when compared with controls (0.25±0.10 vs. 0.31±0.12 ng/ml, P =0.003). Conditional multi-logistic regression analyses after adjustments for potential confounding factors revealed that CCDC80 was a strong and independent protective factor for GDM (ORs <1). In addition, the results of the ROC analysis indicated the CCDC80 exhibited the capability to identify pregnant women with GDM (AUC=0.633). Finally, multivariate regression analyses showed that CCDC80 levels were positively associated with AST, monoamine oxidase, complement C1q, LDL-C, apolipoprotein A1and B, and negatively associated with blood glucose levels at 1 h post- OGTT. Conclusions: Biomarker CCDC80 could be of great value for the development of prediction, diagnosis and therapeutic strategies against GDM in pregnant women.
Comparing video-based versions of Halsted’s ‘See One, Do One’ and Peyton’s ‘4-step Approach’ for teaching surgical skills: a randomized controlled trial
Authors: Seifert Lukas Benedikt , Schnurr Benedikt , Stefanescu Maria-Christina , Sader Robert , Rüsseler Miriam , Sterz Jasmina 
Published: January 2020

Abstract
Background: Teaching complex motor skills at a high level remains a challenge in medical education. Established methods often involve large amounts of teaching time and material. The implementation of standardized videos in those methods might help save resources. In this study, video-based versions of Peyton’s ‘4-step Approach’ and Halsted’s ‘See One, Do One’ are compared. We hypothesized that the video-based ‘4-step Approach’ would be more effective in learning procedural skills than the ‘See One, Do One Approach’. Methods: One-hundred-two naïve students were trained to perform a structured facial examination and a Bellocq’s tamponade with either Halsted’s (n = 57) or Peyton’s (n = 45) method within a curricular course. Steps 1 (Halsted) and 1-3 (Peyton) were replaced by standardized teaching videos. The performance was measured directly (T1) and 8 weeks (T2) after the intervention by blinded examiners using validated checklists. An item-analysis was also carried out. Results: At T1, performance scores significantly differed in favor of the video-based ‘4-step Approach’ (p < 0.01) for both skills. No differences were found at T2 (p < 0.362). The item-analysis revealed that Peyton’s method was significantly more effective in the complex subparts of both skills. Conclusions: The modified video-based version of Peyton’s ‘4-step Approach’ is the preferred method for teaching especially complex motor skills in a large curricular scale. Furthermore, an effective way to utilize Peyton’s method in a group setting could be demonstrated. Further studies have to investigate the long-term learning retention of this method in a formative setting.
The Detrimental Impacts of Negative Age Stereotype on Episodic Memory of Older Adults: Does Social Participation Moderate the Effects?
Authors: Chan Stephen Cheong Yu , Au Alma May Lan , Lai Simon Man Kin 
Published: January 2020

Abstract
Background: Memory performances were shown to be negatively affected by negative age stereotype primes among older adults. The purpose of the study was to examine the effects of negative age stereotype primes on episodic memory using implicit priming intervention and further investigate whether social participation moderate the effects. Methods: A total of 105 community-dwelling older adults residing in Hong Kong were randomly allocated to two experimental conditions. Participants were primed either with negative age stereotypes (n = 53) or neutral words (n = 52) by implicit priming intervention which was framed as reaction task prior to the episodic memory tasks. Other measures such as demographic variables and social participation rate were collected by questionnaire during the delayed recall interval. Results: Results indicated that experimental group performed significantly worse than the control group among all measures in the memory performances. Follow-up analyses showed that social participation moderated the effects of negative age stereotypes in the recognition task. Conclusions: Using a simple scale to measure social participation, we found there was a significant moderating effect of social participation on the effects of negative age stereotype primes on episodic memory, these results provide initial support for social participation might act as an effective strategy against negative age stereotypes. Trial registration ClinicalTrials.gov: NCT04202120 (first posted December 17, 2019), (Retrospectively registered) Keywords: Age stereotypes, episodic memory, older adults, social participation
Trajectories of hospitalization in three groups of patients with incurable gastric cancer identified by hierarchical clustering: A retrospecitve analysis
Authors: Murakawa Yasuko , Sakayori Masato , Otsuka Kazunori 
Published: January 2020

Abstract
Purpose: The therapeutic goal for advanced solid malignancies is not to achieve cure but to prolong survival and maintain quality of life (QOL). To date, no study has reported the trajectory of the QOL throughout the clinical course of a patient with advanced malignancy. As hospitalization is considered a predictor of QOL, we retrospectively analyzed the trajectory of hospitalization in patients with incurable gastric cancer throughout the clinical course. Methods: The data of 85 patients with incurable gastric cancer were collected, including age, sex, Eastern Cooperative Oncology Group (ECOG) Performance Status (PS), treatment, histology, sites of metastases at first consultation, planned and unplanned hospitalization throughout the clinical course , and overall survival (OS). We ranked the patients by OS and hospitalization using a hierarchical clustering analysis. Results: Three clusters were identified corresponding to short, intermediate, and long OS/hospitalization (Clusters 1, 2, and 3, respectively). Patients in Cluster 3 were more likely to have an ECOG PS of 0–2 and receive palliative chemotherapy than the other clusters. No other differences in histology, age, sex, and or extra-peritoneal metastasis sites were observed between the three groups. In Cluster 3, planned hospitalization accumulated gradually during the early clinical phase, while unplanned hospitalization accumulated rapidly in later phases. Conclusions: No specific characteristics were associated with short, intermediate, and long OS/hospitalization. Patients in the long OS/hospitalization group exhibited a rapid accumulation of unplanned hospitalization during the latter clinical course. Further research is needed to identify specific predictors of and measures to avoid a long OS/hospitalization.
Psychometric properties of Integrated Palliative Outcome Scale: Czech standardization and validation
Authors: Vlckova Karolina , Hoschlova Eva , Chroustova Eva , Loucka Martin 
Published: January 2020

Abstract
Background Outcome measurement is an essential part of the evaluation of palliative care and the measurements need to be reliable, valid and adapted to the culture in which they are used. The Integrated Palliative Outcome Scale (IPOS) is a widely used tool for assessing outcomes in palliative care. The aim of this study was to provide Czech version of IPOS and asses its psychometric properties.Methods Patients receiving palliative care in hospice or hospitals completed IPOS and part of the sample also completed Edmonton Symptom Assessment System (ESAS) and Palliative Performance Scale (PPS). The reliability of Czech IPOS was tested with Cronbach alpha (internal consistency) and Intraclass correlation coefficient and Weighted Kappa (test-retest reliability). Construct validity was assessed with factor analysis (Exploratory Factor Analysis) and convergent validity was tested with correlation analysis (Spearman correlation).Results Sample consisted of 140 patients (mean age 72; 90 women; 81% oncologic disease). IPOS internal consistency was 0.789; ICC= 0.88. To study convergent validity, we assessed the correlations of IPOS with ESAS (R= 0.4) and PPS (R= -0.2), however, these results have to be considered preliminary due to the small sample size. Exploratory factor analysis revealed a 2-factor solution on our data. The first factor covers emotional and information needs and the second factor covers physical symptoms.Conclusion Czech IPOS has very good reliability regarding both internal consistency and test-retest reliability. Together with an item analysis results, we can conclude that the Czech adaptation of the tool was successful. The convergent validity needs to be assessed on the larger sample and the proposed 2-factor internal structure of the questionnaire has to be confirmed by using CFA.
The impact of occupational and personal factors on musculoskeletal pain - a cohort study of female teachers, nurses and sonographers
Authors: Arvidsson Inger , Simonsen Jenny Gremark , Andersson Agneta Lindegård , Björk Jonas , Nordander Catarina 
Published: January 2020

Abstract
Background Musculoskeletal pain is common in the general population and constitutes a major public health problem. A large proportion of these conditions may be work related. This study aimed to explore the relative importance of physical, psychosocial and personal factors, for musculoskeletal single- and multisite pain, among women in ordinary professions with a broad variety of occupational exposures. Methods A cohort of 1115 women responded to a questionnaire of ergonomic, psychosocial, personal and life-style factors, and musculoskeletal pain (based on frequency and intensity of complaints in nine anatomical sites), at baseline and at follow-up (mean 28 months later). Sum-scores of ergonomic- and psychosocial factors were created. The importance of the exposures at baseline for multisite pain (≥four pain-sites) at follow-up were estimated using ordinal regression. Multi-exposure Poisson regression models of exposures at baseline were used for outcomes of single-site pain at follow-up, in the neck, the shoulders, the hands, the lower back and the feet. Results High sum-scores of ergonomic- and psychosocial factors at baseline were of importance for multisite pain at follow-up, although the strongest risk factor was the presence of multisite pain at baseline. There was a large fluctuation in number of pain-sites between the two time-points, on the individual level. Eighteen percent of the study population reported multisite pain both at baseline and at follow-up, while only eleven percent did not report any pain, neither at baseline nor at follow-up. Among the single-site outcomes, a high sum-score of ergonomic factors was a risk factor of pain in the neck, the hands and the feet. A high sum-score of psychosocial factors was a risk factor of neck- and shoulder pain. The strongest risk factor was, however, pain in the actual anatomical site at baseline. Only a few of the personal and life-style factors were of importance for subsequent single- and multisite pain. Conclusions An overwhelming majority of the women in common occupations were affected by musculoskeletal pain. Both ergonomic- and psychosocial factors were predictive of single- and multisite pain. The findings points to the need of actions on individual, organizational and societal level.