bluuebunny/crossref_metadata_2025_split huggingface dataset:
The epidemiology of Mayaro virus in the Americas: A systematic review and key parameter estimates for outbreak modelling
Authors: Caicedo Edgar-Yaset , Charniga Kelly , Rueda Amanecer , Dorigatti Ilaria , Mendez Yardany , Hamlet Arran , Carrera Jean-Paul , Cucunubá Zulma M. 
Published: December 2020

AbstractMayaro virus (MAYV) is an arbovirus that is endemic to tropical forests in Central and South America, particularly within the Amazon basin. In recent years, concern has increased regarding MAYV’s ability to invade urban areas and cause epidemics across the region. We conducted a systematic literature review to characterise the evolutionary history of MAYV, its transmission potential, and exposure patterns to the virus. We analysed data from the literature on MAYV infection to produce estimates of key epidemiological parameters, including the generation time and the basic reproduction number, R0. We also estimated the force-of-infection (FOI) in epidemic and endemic settings. Seventy-six publications met our inclusion criteria. Evidence of MAYV infection in humans, animals, or vectors was reported in 14 Latin American countries. Nine countries reported evidence of acute infection in humans confirmed by viral isolation or reverse transcription-PCR (RT-PCR). We identified at least five MAYV outbreaks. Seroprevalence from population based cross-sectional studies ranged from 21% to 72%. The estimated mean generation time of MAYV was 15.2 days (95% CrI: 11.7-19.8) with a standard deviation of 6.3 days (95% CrI: 4.2-9.5). The per-capita risk of MAYV infection (FOI) ranged between 0.01 and 0.05 per year, producing R0 estimates between 1.1 and 2.9 in endemic settings. In an outbreak in Santa Cruz, Bolivia, R0 was estimated at 2.2 (95% CrI: 0.8-4.8). Although MAYV has been identified in urban vectors, there is not yet evidence of sustained urban transmission. MAYV’s enzootic cycle could become established in forested areas within cities similar to yellow fever virus.Author summaryEach year, diseases that are transmitted by mosquitoes cause substantial deaths and disability across the world. We performed a systematic literature review of Mayaro virus (MAYV) and estimated key epidemiological parameters that can be used to improve future outbreak response. We estimated the generation time and basic reproduction number for a historical outbreak. Our results suggest that the force-of-infection of MAYV in endemic settings is low. We did not find evidence of substantial urban transmission of MAYV. Nevertheless, similarities between MAYV and yellow fever virus epidemiology suggest that MAYV could emerge in urban areas. Local transmission of MAYV has never been reported outside of Central and South America. Our results highlight the need to continue monitoring emerging arboviruses in the Americas.
Validation of the IMPEDE VTE Score for Prediction of Venous Thromboembolism in Multiple Myeloma: A Retrospective Cohort Study
Authors: Covut Fahrettin , Ahmed Ramsha , Chawla Sanchit , Ricaurte Frank , Samaras Christy J. , Anwer Faiz , Mejia Garcia Alex V. , Angelini Dana E , Mazzoni Sandra , Faiman Beth , Valent Jason , Khouri Jack 
Published: December 2020

ABSTRACTVenous thromboembolism (VTE) is highly prevalent in Multiple Myeloma (MM) patients, however a reliable VTE prediction tool in MM remains under study. The IMPEDE VTE score has recently emerged as a novel risk prediction tool for VTE in MM but needs external validation in different cohorts. We conducted a retrospective cohort study to validate this score. We reviewed 839 patients who were newly diagnosed with MM between 2010 and 2015 at Cleveland Clinic and included 575 patients in final analysis. The 6-month cumulative incidence of VTE among all patients was 10.7% (95% CI: 8.2 – 13.2) and the c-statistic of the IMPEDE VTE score to predict VTE within 6 months of treatment start was 0.68 (95% CI: 0.61 – 0.75). The 6-month cumulative incidence of VTE was 5.0% (95% CI: 2.1 – 7.9) in the low risk group, compared to 12.6% (95% CI: 8.9% – 16.4%) and 24.1% (95% CI: 12.2 – 36.1) in the intermediate and high risk groups (p<0.001 for both). In addition, a higher proportion of patients in the VTE cohort had ECOG performance status of ≥2 as compared to the no VTE cohort (33% vs. 16%, p=0.001). Other MM characteristics such as stage, immunoglobulin subtype, and cytogenetics were not found to be predictors of VTE. In summary, we have validated the IMPEDE VTE score in our patient cohort and our findings suggest that it can be utilized as a VTE risk stratification tool in prospective studies looking into investigating VTE prophylaxis strategies in MM patients.
Surprising Phenotypic Diversity of Cancer-associated mutations of Gly 34 in the Histone H3 tail
Authors: Lowe Brandon R. , Yadav Rajesh K. , Henry Ryan A. , Schreiner Patrick , Matsuda Atsushi , Fernandez Alfonso G. , Finkelstein David , Campbell Margaret , Kallappagoudar Satish , Jablonowski Carolyn M. , Andrews Andrew J. , Hiraoka Yasushi , Partridge Janet F. 
Published: December 2020

AbstractSequencing of cancer genomes has identified recurrent somatic mutations in histones, termed oncohistones, which are frequently poorly understood. Previously we showed that fission yeast expressing only the H3.3G34R mutant identified in aggressive pediatric glioma had reduced H3K36 trimethylation and acetylation, increased genomic instability and replicative stress, and defective homology-dependent DNA damage repair (Yadav et al., 2017). Here we show that surprisingly distinct phenotypes result from G34V (also in glioma) and G34W (giant cell tumors of bone) mutations, differentially affecting H3K36 modifications, subtelomeric silencing, genomic stability, sensitivity to irradiation, alkylating agents, hydroxyurea and influencing DNA repair. In cancer, only one of thirty alleles encoding H3 is mutated. Whilst co-expression of wild-type H3 rescues most G34 mutant phenotypes, G34R causes dominant hydroxyurea sensitivity and homologous recombination defects, and dominant subtelomeric silencing. Together, these studies demonstrate the complexity associated with different substitutions at even a single residue in H3 and highlight the utility of genetically tractable systems for their analysis.
Novel xylan degrading enzymes from polysaccharide utilizing loci ofPrevotella copriDSM18205
Authors: Linares-Pastén Javier A. , Hero Johan Sebastian , Pisa José Horacio , Teixeira Cristina , Nyman Margareta , Adlercreutz Patrick , Martinez M. Alejandra , Karlsson Eva Nordberg 
Published: December 2020

AbstractPrevotella copriDSM18205 is a bacterium, classified under Bacteroidetes that can be found in the human gastrointestinal tract (GIT). The role ofP. copriin the GIT is unclear, and elevated numbers of the microbe have been reported both in dietary fiber-induced improvement in glucose metabolism but also in conjunction with certain inflammatory conditions. These findings raised our interest in investigating the possibility ofP. coprito grow on xylan, and identify the enzyme systems playing a role in digestion of xylan-based dietary fibers inP. copri, which currently are unexplored. Two xylan degrading polysaccharide utilizing loci (PUL10 and 15) were found in the genome, with three and eight GH-encoding genes, respectively. Three of the eight gene products were successfully produced inEscherichia coli: One monomeric two-domain extracellular enzyme from GH43 (subfamily 12, in PUL10, 60 kDa) and two dimeric single module enzymes from PUL15, one extracellular GH10 (41 kDa), and one intracellular GH43 subfamily 1 enzyme (37 kDa). The GH43_12 enzyme was hydrolysing arabinofuranose residues from different substrates, and a model of the 3D-structure revealed a single arabinose binding pocket. The GH10 (1) and GH43_1 are cleaving the xylan backbone. Hydrolysis products of GH10 (1) were DP2-4, and seven subsites (−3 to +4) were predicted in the 3D-model of the GH10 active site. GH43_1 mainly produced xylose (in line with its intracellular location). Based on our results we propose that in PUL15, GH10 (1) is an extracellular endo-1,4-β-xylanase, that hydrolyses mainly glucuronosylated xylan polymers to xylooligosaccharides (XOS); while, GH43_1 in the same PUL, is an intracellular β-xylosidase, catalysing complete hydrolysis of the XOS to xylose. In PUL10, the characterized GH43_12 is an arabinofuranosidase, with a role in degradation of arabinoxylan, catalysing removal of arabinose-residues on xylan polymers.
Improved Photocleavable Proteins with Faster and More Efficient Dissociation
Authors: Lu Xiaocen , Wen Yurong , Zhang Shuce , Zhang Wei , Chen Yilun , Shen Yi , Lemieux M. Joanne , Campbell Robert E. 
Published: December 2020

AbstractThe photocleavable protein (PhoCl) is a green-to-red photoconvertible fluorescent protein that, when illuminated with violet light, undergoes main chain cleavage followed by spontaneous dissociation of the resulting fragments. The first generation PhoCl (PhoCl1) exhibited a relative slow rate of dissociation, potentially limiting its utilities for optogenetic control of cell physiology. In this work, we report the X-ray crystal structures of the PhoCl1 green state, red state, and cleaved empty barrel. Using structure-guided engineering and directed evolution, we have developed PhoCl2c with higher contrast ratio and PhoCl2f with faster dissociation. We characterized the performance of these new variants as purified proteins and expressed in cultured cells. Our results demonstrate that PhoCl2 variants exhibit faster and more efficient dissociation, which should enable improved optogenetic manipulations of protein localization and protein-protein interactions in living cells.
Predictors of Physical and Mental Health in Healthcare Teams Working with COVID-19 patients: a scoping review protocol
Authors: Aguirre-Duarte Nelson , Øvretveit John , Kenealy Timothy 
Published: December 2020

AbstractIntroductionAs a result of the current pandemic (COVID-19), many clinical teams are exposed to stressful situations that may lead to physical and mental issues for clinical staff themselves (we exclude the effects of personal infection with the virus). Recent studies suggest some predictors could depend on context, notably country and the type of the health system.Methods and AnalysisThis protocol was follows using the PRISMA-ScR guideline (Preferred Reporting Items for Systematic reviews and Meta-Analyses extension for Scoping Reviews), which was revised and approved by the research team. This study aims to identify factors and evidence of the physical, behavioural and mental consequences of sustained clinical practice in a continuing pandemic. Our research seeks to fill this gap in the literature, and the results may suggest to governments, healthcare authorities and healthcare providers appropriate measures to mitigate risks to healthcare workers during a pandemic response.Dissemination and ethicsThe current research design is based on the use of publicly available information and does not require ethical approval. The findings will be disseminated in conferences. Results will be published and additionally shared with relevant local and national authorities.Strengths and Limitations of the studyThis will be the first scoping review to identify factors and evidence of the physical, behavioural and mental consequences of sustained clinical practice in a continuing pandemic with health impacts for clinical staff.The search strategy includes six electronic databases with peer-reviewed literature, as well as a broad range of grey literature sources.Although this study will not require a quality appraisal, which is consistent with the framework proposed by Arksey and O’Malley, the current study will formally assess the studies quality.This scoping review study has been registered with Open Science Framework to enhance its transparency.The search strategy proposed is broad, but the search strategy is limited to articles published in English, Spanish, Portuguese or Italian.
On classification of (n + 6)-dimensional nilpotent n-Lie algebras of class 2 with n ≥ 4
Authors: Jamshidi Mehdi , Saeedi Farshid , Darabi Hamid 
Published: December 2020

PurposeThe purpose of this paper is to determine the structure of nilpotent (n+6)-dimensional n-Lie algebras of class 2 when n≥4.Design/methodology/approachBy dividing a nilpotent (n+6)-dimensional n-Lie algebra of class 2 by a central element, the authors arrive to a nilpotent (n+5) dimensional n-Lie algebra of class 2. Given that the authors have the structure of nilpotent (n+5)-dimensional n-Lie algebras of class 2, the authors have access to the structure of the desired algebras.FindingsIn this paper, for each n≥4, the authors have found 24 nilpotent (n+6) dimensional n-Lie algebras of class 2. Of these, 15 are non-split algebras and the nine remaining algebras are written as direct additions of n-Lie algebras of low-dimension and abelian n-Lie algebras.Originality/valueThis classification of n-Lie algebras provides a complete understanding of these algebras that are used in algebraic studies.
Plant protection practices: how do risk perception, subjective and objective knowledge influence the preference of German consumers
Authors: Lehberger Mira , Becker Christine 
Published: December 2020

PurposeThe purpose of this paper is to identify which plant protection practices consumers prefer and why. The authors focused on beneficial insects, genetically modified (GM) plants, synthetic chemical pesticides, biological plant protection, mechanical-physical plant protection as well as biotechnical plant protection. The authors studied the effects of the risk perception and both subjective and objective knowledge on preference.Design/methodology/approachThe authors collected cross-sectional data from n = 1,223 people living in Germany. For this, the authors used an online panel and quoted participation after age, gender, income and region. The authors used multiple regression analyses and also explored moderation effects of knowledge on risk perception to explain participants' preferences.FindingsThe most preferred plant protection practice in the study sample was the application of beneficial insects. The authors found evidence that risk perception, as well as objective and subjective knowledge, can have a pivotal direct effect on preferences. Additionally, subjective as well as objective knowledge typically moderated the effect of risk perception, depending on the plant protection practice in focus. Overall, the authors found that levels of subjective and objective knowledge of plant protection practices were rather low among German participants.Originality/valueWhile studies on consumer preferences for GM or organic food are abundant, preferences regarding other types of common plant protection practices are hardly investigated. To tackle this research gap, the authors focused on six different and common plant protection practices and compare results.
EntreCompEdu, a professional development framework for entrepreneurial education
Authors: Grigg Russell 
Published: December 2020

PurposeThe purpose of this paper is to present a new professional development framework (EntreCompEdu) that identifies the competencies educators need to promote entrepreneurial education in primary, secondary and vocational settings.Design/methodology/approachThis conceptual paper explores the relationships between the framework's various constructs to articulate its rationale and value. Its design was informed by literature review and critical feedback from an advisory group of European and national policymakers, university staff, teachers and education consultants. It is currently in a pilot stage.FindingsThe paper proposes a new model in the field resting on six pedagogical principles. These are translated into five areas of competence: entrepreneurial knowledge and understanding, planning and organization, teaching and training, assessment, and professional learning.Research limitations/implicationsThis paper only describes the conceptual thinking and contents of EntreCompEdu based on a limited literature review. Empirical research is necessary to assess the impact of EntreCompEdu on teaching. There are implications for building a network to support educators' professional development.Practical implicationsEntreCompEdu and its training materials offer educators structure and guidance to develop their competences. These are available in open access format, viahttps://www.entrecompedu.eu. Participants will have access to a bank of effective teaching practices and support network across Europe.Social implicationsCollaboration is essential to effective entrepreneurial education, with EntreCompEdu facilitating a support network across Europe.Originality/valueEntreCompEdu is an original response to a policy problem, namely the need for a professional development framework to support the implementation of EntreComp. It is timely given calls to pay further attention to teachers' professional development and widen the appeal of entrepreneurial education.
Criteria for selecting partner cities in a national-level resilience network
Authors: Elkhidir Elrasheid , Mannakkara Sandeeka , Wilkinson Suzanne 
Published: December 2020

Purpose
The purpose of this paper is to establish the factors affecting the selection of a suitable partner city for resilience building at the national level.

Design/methodology/approach
An exploratory sequential research was adopted using New Zealand as a case study. Data were collected through focus groups and semi-structured interviews and subsequently validated through an online survey.

Findings
The study confirmed that the criteria for selecting partner cities for collaboration and knowledge sharing on resilience were similarity of hazards, geographic proximity, city resources and priorities, resilience performance, city size and demographics, previous relationship, willingness to collaborate and similar industries.

Practical implications
The findings of this paper will help guide cities that are interested in developing national-level resilience partnerships through the process of selecting the most suitable partner cities.

Originality/value
Despite the existence of international intercity resilience networks, there is a lack of information on the criteria affecting the selection of suitable resilience partner cities at the national level. This paper addresses this gap and offers informed decision-making criteria for cities to consider.
Influence of tourists’ sociodemographic characteristics on their perception of destination personality and emotional experience of a city break destination
Authors: Šagovnović Ivana , Kovačić Sanja 
Published: December 2020

PurposeThe purpose of this paper is to investigate the influence of tourists’ sociodemographic characteristics on their perception of destination personality and emotional experience on the example of the city break tourism destination.Design/methodology/approachTo examine this relationship, survey research was conducted on a sample of 203 national and international tourists who visited Novi Sad, the second-largest city in Serbia.FindingsResearch results confirmed the role of travelers’ sociodemographic variables in shaping their emotional experience and destination personality perception. The findings pointed out significant divergences in the perception of emotional experience in the case of respondents’ education level, previous visits to the city and travel companion. On the other hand, the analysis showed that repeat visitors significantly differed from first-time visitors regarding destination personality perception. In addition, differences in both destination personality and emotional experience assessment were found between national and international city break travelers.Originality/valueThe current study is first to focus on the role of travelers’ sociodemographic variables in simultaneous modeling of their perception of destination personality and emotional experience within the city break destination context. Besides, results revealed some new influencing factors of both destination personality and emotional experience perception, thus contributing to the existing tourism literature. In addition, this paper offers useful practical implications for city break marketers to adapt promotional activities, more effectively present the desired brand personality of the city to different sociodemographic categories of tourists and sustain repeat tourists’ perception of Positive surprise.
A road traffic accidents prediction model for traffic service robot
Authors: Zhang Chaohui , Li Yijing , Li Tian 
Published: December 2020

PurposeIn recent years, the demand for road traffic has continued to increase, but the casualties and economic losses caused by traffic accidents have also remained high. Therefore, the use of social service robots to manage, supervise and warn real-time traffic information has become an inevitable trend of traffic safety management.Design/methodology/approachIn order to explore the inherent objective development law of road traffic accidents, in this paper, the factor analysis (FA) is used to explore the main influencing factors of traffic accidents, then the random forest algorithm is applied to build an FA–RF-based road traffic accident severity prediction model to predict two- and three-category accidents.FindingsBy comprehensively comparing the classification results of the two- and the three-category accident prediction, it also finds that due to the intersection between injuries and fatalities and the lack of necessarily external environmental information, the FA–RF model has a large degree of misjudgment for injuries and fatalities. Therefore, it is recommended to establish a real-time autonomous information communication mechanism between different kinds of social robots, which can improve the prediction of traffic accidents.Originality/value(1) A fusion model of FA–RF is considered to predict traffic accidents, which can be applied in traffic service robot. (2) It is recommended to establish a real-time autonomous information communication mechanism between different kinds of social robots, which can improve the prediction of traffic accidents.
On the motivational nature of authentic leadership practices: a latent profile analysis based on self-determination theory
Authors: Levesque-Côté Julie , Fernet Claude , Morin Alexandre J.S. , Austin Stéphanie 
Published: December 2020

PurposeAlthough one of the central premises of authentic leadership theory is that authentic leaders mobilize their followers, the underlying motivational mechanisms of this process remain poorly understood. Drawing on self-determination theory, this study aims to fill that gap by examining authentic leadership practices (ALP) as theoretical antecedents of employees' motivation profiles.Design/methodology/approachLatent profile analyses conducted on a sample of 501 employees revealed four profiles: self-determined, unmotivated, highly motivated and moderately motivated.FindingsALP were associated with a higher likelihood of membership into the most adaptive motivation profiles. Employees in these profiles displayed more optimal job functioning: higher organizational commitment and performance, and lower intentions to leave their organization.Originality/valueThese findings underscore the predictive power of autonomous motivation for employee functioning and provide new insights into how ALP can improve work motivation, and hence job functioning. Our results account not only for how ALP affects the complete range of behavioral regulations at work but also the different patterns in which these regulations combine within employees.