bluuebunny/crossref_metadata_2025_split huggingface dataset:
Smartband use during enhanced recovery after surgery facilitates inpatient recuperation following minimally invasive colorectal surgery
Authors: Yin Tzu-Chieh , Huang Ching-Wen , Tsai Hsiang-Lin , Su Wei-Chih , Ma Cheng-Jen , Chang Tsung-Kun , Wang Jaw Yuan 
Published: June 2020

Abstract
Background Enhanced recovery after surgery (ERAS) is valuable in perioperative care for its ability to improve short-term surgical outcomes and facilitate patient recuperation after major surgery. Early postoperative mobilization is a vital component of the integrated care pathway and is a factor strongly associated with successful outcomes. However, early mobilization still has various definitions and lacks specific strategies. Methods Patients who underwent minimally invasive surgery for colorectal cancer followed our perioperative ERAS program, including mobilization from the first postoperative day. After perioperative care skills were improved in our well-established program, compliance, inpatient surgical outcomes, and complications associated with adding smartband use were evaluated and compared with the outcomes for standard protocol. Quality of recovery was evaluated using patient-rated QoR-40 questionnaires the day before surgery, on postoperative day 1 and 3, and on the day of discharge. Results Smartband use after minimally invasive colorectal surgery failed to increase compliance with early mobilization or reduce the occurrence of postoperative complications significantly compared with standard ERAS protocol. However, when smartbands were utilized, quality of recovery was optimized and patients returned to their preoperative status earlier, at postoperative day 3. The duration of theoretical hospital stays and hospital stays of uncomplicated patients wearing smartbands was also reduced by 1.1 and 0.9 days, respectively ( P = 0.0091 and 0.049). Conclusions Smartbands enable enhanced communication between patients and surgical teams and strengthen self-management in patients undergoing minimally invasive colorectal resection surgery. Accelerated recovery to preoperative functional status can be facilitated by integrating smartbands into the process of early mobilization during ERAS.
Dexmedetomidine alleviates LPS-induced neuronal dysfunction via AKT/GSK-3β/CRMP-2 pathway
Authors: Zeng Wei , Zhang Chunyuan , Long Qingshan , Li Yalan 
Published: June 2020

Abstract
Background

Dexmedetomidine (Dex), an α2-adrenergic receptor agonist, shows intervention effect on cognitive dysfunction in elderly patients after general anesthesia. However, the underlying pathological mechanism remains to be further elucidated. The aim of the study was to investigate effects of Dex on LPS-induced neuronal damages in cultured hippocampal neurons.
Methods

We applied LPS to mimic the post-surgical inflammation in cultured hippocampal neuron. Cultured hippocampal neurons were treated with LPS in a dose- and time- dependent manner, then were administrated with or without Dex. The neuronal morphology including neurite outgrowth and synaptic transmission was observed, and mEPSCs was recorded by electrophysiological patch clamp. Cell lysates were subjected for western blot to assess the explore the potential underlying mechanism.
Results

Compared to control group, administration of LPS significantly impaired the neurite outgrowth in a concentration- and time-dependent manner. Dex treatment markedly reversed LPS-induced impairment of neurite outgrowth in hippocampal neurons. Electrophysiological patch clamp results showed that LPS induced synaptic transmission dysfunction, which could be restored after Dex addition. Furthermore, western blotting assays showed that LPS suppressed the AKT/GSK-3β/CRMP-2 signaling pathway and Dex administration significantly re-activated this pathway to encounter the inhibitory effect of LPS.
Conclusion

Addition of Dex showed significant protection effect on LPS-induced hippocampal neuron damages, including neurite outgrowth and synaptic transmission. Dex functioned via the activation of GSK-3β/CRMP-2 signaling pathway to alleviate LPS-induced neurological dysfunctions.
­­Awareness of the Fruits and Veggies (FNV) Campaign Among Target Audiences in California and Virginia: A Cross-sectional Study
Authors: Englund Tessa Rhianon , Hedrick Valisa Ellen , Patiño Sofia Rincón-Gallardo , Kennedy Lauren Elaine , Hosig Kathryn Wright , Serrano Elena Lidia , Kraak Vivica Ingrid 
Published: June 2020

Abstract
Background

The Partnership for a Healthier America launched the branded Fruits & Veggies (FNV) Campaign in 2015 to promote fruit and vegetable sales and intake to mothers and teens in two US pilot media market areas: Fresno, California and Hampton Roads, Virginia. Limited evidence is available on the effectiveness of marketing strategies to influence consumers’ fruit and vegetable demand. This cross-sectional study assessed associations between awareness of the FNV Campaign and attitudes, beliefs, intentions, and behaviors related to fruit and vegetable purchases and consumption among targeted audiences from the California and Virginia pilot markets.
Methods

Data were collected using an online survey between February and July 2017 using a purposive sample (n = 1604) of youth aged 14–20 years (n = 744) and mothers aged 21–36 years (n = 860).
Results

Approximately 20% (n = 315) of respondents reported awareness of the FNV Campaign. Youth aware of the FNV Campaign (n = 167) reported higher intentions to buy (p = 0.003) and eat (p = 0.009) fruits and vegetables than unaware youth respondents. Mothers aware of the FNV Campaign (n = 148) reported greater encouragement for friends and family to eat fruits and vegetables (p = 0.013) and had approximately 1.5 times higher odds of reporting trying a new fruit or vegetable (p = 0.04) than unaware mothers. Daily fruit and vegetable intake frequency did not differ by Campaign awareness.
Conclusions

Awareness of the FNV Campaign was associated with limited but positive cognitive and behavioral outcomes among target audience respondents. The present findings can inform future research to enhance understanding and improve effectiveness of FNV Campaign marketing practices as it expands throughout new markets nationwide.
The Effect of Temperature Upon Transmission Of&nbsp;COVID-19 : Australia And Egypt Case Study
Authors: Anis Adly 
Published: June 2020

Abstract
Background: Several previous studies have recognized the effect of air temperature on the survival and transmission of viruses and germs. The current study investigated the effect of air temperature on the transmission of coronavirus covid-19 by monthly temperature averages maps analyzing.Methods: The study demonstrated the relationship between temperature and transmission speed of Covid-19 virus, It confirmed that the most appropriate average temperature for virus activity and transmission ranges between 13-24 ° C, by analyzing the maps of monthly temperature averages in Egypt and Australia.Results: The study reached, through cartographic analysis, to confirm the relationship between temperature and increase in the number of confirmed cases of covid-19, This study confirmed that the most appropriate average temperature for virus activity and transmission ranges between 13-24 ° C, by analyzing the maps of monthly temperature averages in Egypt and Australia.Conclusions: Results support that the most appropriate average temperature for the survival transmission of COVID-19 ranges between 13-24 ° C. Australia and Egypt are models to confirm the relationship between temperature and COVID-19 activity and spread.
Ecological and Floristic Study of Hirmi Forest, Tigray Region, Northern Ethiopia
Authors: Girmay Mehari , Bekele Tamrat , Demissew Sebsebe , Lulekal Ermias 
Published: June 2020

Abstract
Background: The Dryland area in Ethiopia contributes large vegetation resources. However, the landmass has received less attentioneven if it has high ecological, environmental and economic importance. The present study was conducted in Hirmi forest, which is one of the dry forest in Northwestern, Ethiopia; to study the floristic composition, plant community types, community-environment relation, vegetation structure and regeneration status of the forest.Method: Vegetation and environmental data were collected from 80 sampling plots having equal size of 25m×25m were designated as the main plots. Within the main plot, five 2mx2m subplots were laid to record seedlings, saplings, and herbaceous species. Furthermore, within each subplot, soil samples were collected to analyze the relationship of edaphic parameters with the plant community. DBH, height, BA, density, vertical structure and frequency were computed. Floristic diversity and evenness were computed using Shannon diversity and Evenness indices. The plant community types and vegetation-environment relationships were analyzed using classification and ordination tools in R package (ver. 3.6.1), respectively.Result: A total of 171 vascular plant species belonging to 135 genera and 56 families were recorded. About 5.85% of the species were endemic/near endemic to Ethiopia. Woody species (48%) were dominant over herbs (45.6%) and liana (6.4%). The highest number of species was recorded in plant families of Fabaceae (16.37%) and Poaceae (11.7%) followed by Asteraceae (7.02%) and Combretaceae (3.5%). Five plant communities were identified and positively correlated with altitude, slope, sand, silt, soil organic matter, total Nitrogen and disturbance. Vegetation structure reveals that, large number individual species were categorized in the lower class of DBH, BA, frequency and height. The highest Shannon diversity index and evenness values of the study area were 4.21 and 0.95, respectively. Anogeissus leiocarpa, Combretum hartmannianum, Ziziphus mucronata, Terminalia macroptera, Acacia polyacantha were the species with high important value.Conclusion: the study shows Hirmi forest has high species diversity, richness, and endemism. However, due to various disturbances, it is under poor regeneration status that requires urgent conservation measures. Integrated forest management, monitoring, applying restoration techniques by considering the significant environmental factors associated with the species diversity, IUCN threat and regeneration status of the species is recommended to preserve the study forest.
GDF-15 as a biomarker of aging
Authors: Liu Huan , Huang Yun , Lyu Yongnan , Dai Wen , Tong Yongqing , Li Yan 
Published: June 2020

Abstract
Background: The aging process is accompanied by the gradual development of chronic systemic inflammation (inflamm-aging). Growth differentiation factor-15 (GDF-15) is associated with inflammation and known to be a stress-induced factor. The present study aimed to explore the association of GDF-15 with aging.Methods: In this cross-sectional study, serum GDF-15, hematological parameters, and biomedical parameters were determined in 120 healthy individuals (23-83 years old, males). Three telomere related parameters, including telomere length, telomerase activity, and the expression of human telomerase reverse transcriptase (hTERT) mRNA were also quantified.Results: The older group has a higher levels of GDF-15 and lower expression of hTERT mRNA, and PBMC telomerase activity (p<0.001). In individuals with high GDF-15 levels, they were older, and presented with the lower level of hTERT mRNA and T/S ratio (p<0.01). Spearman correlation analysis shows that GDF-15 positively correlated with age (r=0.664, p<0.001), and negatively correlated with telomere length (r=-0.434, p<0.001), telomerase activity (r=-0.231, p=0.012), and hTERT mRNA (r=-0.206, p=0.024). Furthermore, in multivariate regression analysis, GDF-15 levels showed a statistically significant linear and negative relationship with PBMC telomerase activity (β-coefficient=-0.583, 95% CI -1.044 to -0.122, p=0.014), telomere length (β-coefficient=-0.200, 95% CI -0.305 to -0.094, p<0.001), and hTERT mRNA (β-coefficient=-0.207, 95% CI -0.312 to -0.102, p<0.001) after adjusting for confounders.Conclusions: In conclusion, our results show that circulating GDF-15 is the potential biomarkers of aging that may influence the risk and progression of multiple aging conditions.
Analysis on correlation between the Earth's Rotation and Earthquakes Preceding the Occurrence of the 2019 M7.1 Ridgecrest Earthquake
Authors: Li Yane , Chen Xuezhong 
Published: June 2020

Abstract
For earthquakes ( M ³2.0) occurring in and around the rupture zone of the M7.1 Ridgecrest earthquake between January 1990 and June 2019, we analyzed the correlation between the phase of Earth's rotation and the occurrence of earthquakes. The Schuster's test and the two-rate Poisson model were used to statistically examined the correlation between the phase of Earth's rotation and the occurrence of earthquakes. For the Schuster's test,the results were judged based on the P -value. When the P -value is smaller, the correlation is higher. In general, if P £ 5%,the correlation is significant. For the two-rate Poisson model,the results were evaluated by R . When the 95% confidence lower limit on R ( R L ) is over one, earthquakes could be non-random. We calculated the temporal variation on the P -value for earthquakes that occurred in the study region and found some lower P -values,corresponding significant correlation, for earthquakes (2.0≤ M ≤2.4) occurring before the M 7.1 event. When the P -value reached to the lowest,earthquakes are prone to occur when the Earth's acceleration of rotation is increasing or near the peak acceleration.This correlation is also confirmed by the two-rate Poisson model. The spacial distribution of the P -values for earthquakes (2.0≤ M ≤2.4) occurring from Jul. 2013 to Jun. 2019 shows that areas of low P -value were located mainly in and around the northwest segment of the rupture zone of the M 7.1 Ridgecrest earthquake. The lower P -value obtained in this study uncovers the Earth's rotation-triggered earthquakes in and around the rupture zone prior to the M 7 .1 Ridgecrest earthquake,is possible to be considered as one of its precursors.
Effects of Microbial Fertilizer on the Growth, Physiology, and Chlorophyll Fluorescence Response of Spinach Seedlings
Authors: zhang beibei , Jing Qi , Zhang Hui , Wang Jiaxuan , Yang Yang 
Published: June 2020

Abstract
We investigated the effects of different types of bacillus on the growth, physiological characteristics, and chlorophyll fluorescence of spinach seedlings in the soil environment of a Qinxi demonstration garden in Taibai County. Five different fertilization treatments were conducted in the growing room: no fertilizer (CK), addition of Bacillus subtilis (Bs, T1), addition of Bs and Bacillus mucilaginosus (Bm) (T2), addition of Bs and Bacillus amyloliquefaciens (Ba) (T3), and addition of Bs and Ba (T4). There were significant differences in the plant height (PH), leaf length (LL), and leaf weight (LW) of the spinach seedlings (F=54.37, 13.30, and 46.03, respectively; P<0.01). The growth and physiological characteristics of the spinach seedlings attained a maximum under the Bs and Bm treatments. There were differences in the activities of the PSII reaction centers between the five treatments. Under the Bs and Bm treatments, the ABS/RC, TR0 /RC, and ET0 /RC increased significantly, while the DI0 /RC decreased. The OJIP curve increased under different types of fertilization, and the growth trends under the Bs and Bm treatments were the largest. The leaf light response curve (LC) increased significantly under the Bs and Bm treatments. The plant growth characteristics (LL, LW, PH) were positively correlated with the J-I-P test chlorophyll fluorescence parameters (PIABS, φP0, φE0, ψ0, TR0/RC, and ET0/RC), and negatively correlated with φD0 and DI0/RC. The leaf physiological characteristics (SP, SC, Chla, Chlb, Chla+b, Chla/b, and WP) were positively correlated with the J-I-P test chlorophyll fluorescence parameters (PIABS, φP0, φE0, ψ0, ABS/RC, TR0 /RC, and ET0 /RC), and negatively correlated with φD0 and DI0 /RC. The leaf MDA was significantly positively correlated with φD0 and DI0 /RC, and positively correlated with other J-I-P test chlorophyll fluorescence parameters. The Bs and Bm treatments promoted the growth of the spinach seedlings and improved the adaptability of the crops to soil by enhancing the effective phosphorus utilization rate.
Transcriptome Analyses on Compound Material Regulating Saline Stress and Alkaline Stress on Cotton
Authors: An Mengjie , Wang Xiaoli , Chang Doudou , Wang Shuai , Hong Dashuang , Fan Hua , Wang Kaiyong 
Published: June 2020

Abstract
Background: Soil salinization and alkalinization are the main factors that affect the agricultural productivity in the world. Evaluating the persistence of the modifier applied in field soils is an important part of the regulation of saline stress and alkaline stress. Result: To determine the molecular mechanism of cotton’s responses to the regulation of saline stress and alkaline stress by the modifier, in this study, cotton was planted in the salinized soil (NaCl 8g kg-1) and alkalized soil (Na2CO3 8g kg-1) after application of the modifier, and ion content, physiological characteristics, and transcription and sequencing of new leaves during the flowering and boll-forming stage of cotton were analyzed. The results showed that compared with saline stress, alkaline stress increased the content of Na+, K+, SOD, and MDA in leaves, and the application of modifier reduced the content of Na+ but increased the K+/Na+ ratio, the activities of SOD, POD, CAT, and REC. Transcriptome analysis revealed that after the application of the modifier, the Na+/H+ exchanger gene in cotton leaves was down-regulated, the K+ transporter, K+ channel and POD genes were up-regulated. Besides, the down-regulation of genes related to lignin synthesis in phenylalanine biosynthesis pathway was consistent with the study results of ion content and physiological characteristics in leaves. The quantitative analysis with PCR proved the reliability of the results of RNA sequencing.Conclusion: These findings indicate that the modifier alleviated saline stress and alkaline stress on cotton by regulating candidate genes in key biological pathways, which improves our understanding of the molecular mechanism of the modifier regulating saline stress and alkaline stress.
SAGA CORE subunit Spt7 is required for correct Ubp8 localization, chromatin association and deubiquitinase activity
Authors: Nuno-Cabanes Carme , Garcia-Molinero Varinia , Martín-Expósito Manuel , Gas Maria-Eugenia , Oliete-Calvo Paula , Garcia-Oliver Encar , Iglesia-Vaya María De la , Rodriguez-Navarro Susana 
Published: June 2020

Abstract
BackgroundHistone H2B deubiquitination is performed by numerous deubiquitinases in eukaryotic cells including Ubp8, the catalytic subunit of the tetrameric deubiquitination module (DUBm: Ubp8; Sus11; Sgf11; Sgf73) of the Spt-Ada- Gcn5 acetyltransferase (SAGA). Ubp8 is linked to the rest of SAGA through Sgf73 and is activated by the adaptors Sus1 and Sgf11. It is unknown if DUBm/Ubp8 might also work in a SAGA-independent manner.ResultsHere we report that a tetrameric DUBm is assembled independently of the SAGA-core components SPT7, ADA1 and SPT20. In the absence of SPT7, i.e. independent of the SAGA complex, Ubp8 and Sus1 are poorly recruited to SAGA-dependent genes and to chromatin. Notably, cells lacking Spt7 or Ada1, but not Spt20, show lower levels of nuclear Ubp8 than wild type cells, suggesting a possible role for SAGA CORE subunits in Ubp8 localization. Last, deletion of SPT7 leads to defects in Ubp8 deubiquitinase activity in in vivo and in vitro assays.ConclusionsCollectively, our studies show that a stable DUBm is assembled regardless of SAGA integrity; however its function and localization is affected by the absence of Spt7 or Ada1.
Regulator of Cullins-1 (ROC1) Negatively Regulates the Gli2 Regulator SUFU to Activate the Hedgehog Pathway in Bladder Cancer
Authors: Wang Wei , Qiu Jianxin , Qu Pin , Chen Hui , Lan Jianyun , Chen Haitao , Li Lei , Gu Min 
Published: June 2020

Abstract
Background: The regulator of cullins-1 (ROC1) is an essential subunit in the Cullin-RING ligase (CRL) protein complex and was shown to be critical in bladder cancer cell survival and malignant progression. This study aimed to explore the regulatory mechanism of ROC1 in bladder cancer malignant progression. Methods: This study explored the underlying mechanisms using both in vitro and in vivo experiments. The expression of the components of Sonic Hedgehog (SHH) pathway was determined by western blotting analysis. ROC1 expression in human tumours was evaluated by immunohistochemical analysis. Results: The data showed that ROC1 overexpression promoted growth of bladder cancer cells, whereas knockdown of ROC1 expression had an opposite effect in bladder cancer cells. Mechanistically, ROC1 was able to target SUFU for ubiquitin-dependent degradation, allowing the Gli2 release from the SUFU complex to activate SHH pathway. Furthermore, knockdown of SUFU expression partially rescue the ROC1 knockdown-suppressed SHH activity as well as cancer cell growth inhibition. At ex vivo, tissue microarray analysis of human bladder cancer specimens revealed an positive association of ROC1 expression with the SHH pathway activity. Conclusion: The current study demonstrated the dysregulation of ROC1-SUFU-GLI2 axis played an important role in bladder cancer progression and targeting of ROC1 expression is warranted further investigation as a novel strategy for future control of bladder cancer.
The Effect of Different Levels of Prostate-Specific Antigen and Prostate Imaging Report and Data System Scores on Logistic Regression Model to Predict Clinically Significant Prostate Cancer—A Single Center Retrospective Study
Authors: Zhang Loudong , Zhu Hua , Gu Donghua , Pan Xiaodong , zheng bing 
Published: June 2020

Abstract
Background: At present, there are various clinical regression models for predicting prostate cancer. But what about the diagnostic effectiveness of these models in different parameter ranges, and are the models applicable to everyone? This study aimed to study the influence of different levels of prostate-specific antigen (PSA) and Prostate Imaging Report and Data System version 2 (PI-RADS v2) scores on the regression model to predict clinically significant prostate cancer (csPCa).Methods: This retrospective study screened 251 patients from our hospital, who were divided into different groups. The regression model was established for each group to predict csPCa, and the effects of PSA and PI-RADS scores on each model were analyzed through the diagnostic effects of the model.Results: In patients with lower PSA scores, although the model was less sensitive than PSA, the AUC of the model was much greater. With the rise of PSA, the sensitivity of the model surpassed that of PSA, while the specificity became the opposite, and the AUC gap also gradually decreased. In the group with low PI-RADS score, the sensitivity and specificity of PI-RADS were lower than the model, and the gap was larger. Although the gap between the two gradually decreased with the increase of PI-RADS, the diagnostic efficiency of the model was still slightly larger than that of pure PI-RADS.Conclusion: As the PSA and PI-RADS v2 scores increase, the diagnostic advantages of the regression model will gradually decrease. However, for patients with low levels of PSA and PI-RADS scores,the regression model is less affected by PSA and PI-RADS, and can better utilize its clinical diagnostic advantages.