bluuebunny/crossref_metadata_2025_split huggingface dataset:
Examination of Aerosol-Induced Convective Invigoration Using Idealized Simulations
Authors: Cotton William R. , Walko Robert 
Published: October 2020

Abstract
Idealized large-eddy simulations (LESs) are performed of deep convective clouds over south Florida to examine the relative role of aerosol-induced condensational versus mixed-phase invigoration to convective intensity and rainfall. Aerosol concentrations and chemistry are represented by using output from the GEOS-Chem global atmospheric chemistry model run with and without anthropogenic aerosol sources. The results clearly show that higher aerosol concentrations result in enhanced precipitation, larger amounts of cloud liquid water content, enhanced updraft velocities during the latter part of the simulation, and a modest enhancement of the latent heating of condensation. Overall, our results are consistent with the concept that convective cloud invigoration is mainly due to condensational invigoration and not primarily to mixed-phase invigoration. Furthermore, our results suggest that condensational invigoration can result in appreciable precipitation enhancement of ordinary warm-based convective clouds such as are common in locations like south Florida.
Different Impacts of the East Asian Winter Monsoon on the Surface Air Temperature in North America during ENSO and Neutral ENSO Years
Authors: Ma Tianjiao , Chen Wen , Graf Hans-F. , Ding Shuoyi , Xu Peiqiang , Song Lei , Lan Xiaoqing 
Published: October 2020

AbstractThe present study investigates different impacts of the East Asian winter monsoon (EAWM) on surface air temperature (Ts) in North America (NA) during ENSO and neutral ENSO episodes. In neutral ENSO years, the EAWM shows a direct impact on the Ts anomalies in NA on an interannual time scale. Two Rossby wave packets appear over the Eurasian–western Pacific (upstream) and North Pacific–NA (downstream) regions associated with a strong EAWM. Further analysis suggests that the downstream wave packet is caused by reflection of the upstream wave packet over the subtropical western Pacific and amplified over the North Pacific. Also, the East Asian subtropical westerly jet stream (EAJS) is intensified in the central and downstream region over the central North Pacific. Hence, increased barotropic kinetic energy conversion and the interaction between transient eddies and the EAJS tend to maintain the circulation anomaly over the North Pacific. Therefore, a strong EAWM tends to result in warm Ts anomalies in northwestern NA via the downstream wave packet emanating from the central North Pacific toward NA. A weak EAWM tends to induce cold Ts anomalies in western-central NA with a smaller magnitude. However, in ENSO years, an anomalous EAJS is mainly confined over East Asia and does not extend into the central North Pacific. The results confirm that the EAWM has an indirect impact on the Ts anomalies in NA via a modulation of the tropical convection anomalies associated with ENSO. Our results indicate that, for seasonal prediction of Ts anomalies in NA, the influence of the EAWM should be taken into account. It produces different responses in neutral ENSO and in ENSO years.
An updated explanation of ancestral karyotype changes and reconstruction of evolutionary trajectories to form Camelina sativa chromosomes
Authors: Zhang Zhikang , Meng Fanbo , Sun Pengchuan , Yuan Jiaqing , Gong Ke , Liu Chao , Wang Weijie , Wang Xiyin 
Published: October 2020

Abstract
Background
Belonging to lineage I of Brassicaceae, Camelina sativa is formed by two hybridizations of three species (three sub-genomes). The three sub-genomes were diverged from a common ancestor, likely derived from lineage I (Ancestral Crucifer karyotype, ACK). The karyotype evolutionary trajectories of the C. sativa chromosomes are currently unknown. Here, we managed to adopt a telomere-centric theory proposed previously to explain the karyotype evolution in C. sativa.

Results
By characterizing the homology between A. lyrata and C. sativa chromosomes, we inferred ancestral diploid karyotype of C. sativa (ADK), including 7 ancestral chromosomes, and reconstructed the evolutionary trajectories leading to the formation of extant C. sativa genome. The process involved 2 chromosome fusions. We found that sub-genomes Cs-G1 and Cs-G2 may share a closer common ancestor than Cs-G3. Together with other lines of evidence from Arabidopsis, we propose that the Brassicaceae plants, even the eudicots, follow a chromosome fusion mechanism favoring end-end joining of different chromosomes, rather than a mechanism favoring the formation circular chromosomes and nested chromosome fusion preferred by the monocots.

Conclusions
The present work will contribute to understanding the formation of C. sativa chromosomes, providing insight into Brassicaceae karyotype evolution.
Comparative transcriptome analysis reveals that PCK1 is a potential gene affecting IMF deposition in buffalo
Authors: Huang Jieping , Feng Xue , Zhu Ruirui , Guo Duo , Wei Yutong , Cao Xiaodan , Ma Yun , Shi Deshun 
Published: October 2020

Abstract
Background
In China, although buffaloes are abundant, beef is mainly obtained from cattle, and this preference is mainly attributed to the low intramuscular fat (IMF) content of buffalo. Genetic factors are an important driver that affects IMF deposition.

Results
To reveal the intrinsic factors responsible for the low IMF content of buffalo, mRNA expression patterns in muscle and adipose tissue between buffalo and cattle were characterized by RNA sequencing analysis. The IMF content in Nanyang cattle was higher than that in Xinyang buffalo. A total of 1566 mRNAs expressed in adipose tissue showed differential expression between the longissimus dorsi muscles of buffalo and cattle. Functional annotation suggested a difference in the glycolysis/gluconeogenesis pathway between the two species. The results of RT-qPCR analysis and gain-of-function experiments confirmed the positive association between the IMF content and phosphoenolpyruvate carboxykinase 1 (PCK1) expression in buffalo. In both mouse C2C12 cells and cultured bovine myocytes, the activity of the PCK1 promoter in buffalo is lower than that in cattle. However, in mouse 3T3-L1 adipocytes and cultured bovine adipocytes, the activity of PCK1 in buffalo promoter is higher than that in cattle.

Conclusions
These results indicate the important role of PCK1 in buffalo IMF deposition and illustrate the differences between buffalo and cattle promoter activity that drive PCK1 expression. This research helps to establish a foundation for further studies investigating IMF deposition in buffalo.
Identification of fungi in Tunisian olive orchards: characterization and biological control potential
Authors: Gharsallah Houda , Ksentini Ines , Naayma Sourour , Hadj Taieb Karama , Abdelhedi Nour , Schuster Christina , Triki Mohamed Ali , Ksantini Mohieddine , Leclerque Andreas 
Published: October 2020

AbstractBackgroundOlive production is the main agricultural activity in Tunisia. The diversity of fungi was explored in two different olive groves located in two distant geographical zones in Sfax (Tunisia) with different management practices.ResultsFungal isolation was made from soil and the major olive tree pests, namely the Olive fly,Bactrocera oleaeGmelin (Diptera: Tephritidae), and the Olive psyllid,Euphyllura olivinaCosta (Homoptera: Psyllidae). A total of 34 fungal isolates were identified according to their phenotypic, genotypic, biochemical and biological activities. Twenty fungal species were identified belonging to six different genera (Alternaria,Aspergillus,Cladosporium,Fusarium,LecanicilliumandPenicillium) by the analysis of their ITS1–5.8S–ITS2 ribosomal DNA region. Different bioassays performed in this work revealed that 25/34 (73.5%) of the identified fungal isolates showed an entomopathogenic and/or antagonistic activity, 9/34 (26.5%) of them displayed phytopathogenic features.ConclusionsFungal species that showed entomopathogenic and/or antagonistic potentialities and that are non-phytopathogenic, (17/34; 50%) of our fungal isolates, could be explored for olive protection against fungal diseases and pests, and might have a future application as biocontrol agents.
Long non-coding RNAs: emerging players regulating plant abiotic stress response and adaptation
Authors: Jha Uday Chand , Nayyar Harsh , Jha Rintu , Khurshid Muhammad , Zhou Meiliang , Mantri Nitin , Siddique Kadambot H. M. 
Published: October 2020

Abstract
Background
The immobile nature of plants means that they can be frequently confronted by various biotic and abiotic stresses during their lifecycle. Among the various abiotic stresses, water stress, temperature extremities, salinity, and heavy metal toxicity are the major abiotic stresses challenging overall plant growth. Plants have evolved complex molecular mechanisms to adapt under the given abiotic stresses. Long non-coding RNAs (lncRNAs)—a diverse class of RNAs that contain > 200 nucleotides(nt)—play an essential role in plant adaptation to various abiotic stresses.

Results
LncRNAs play a significant role as ‘biological regulators’ for various developmental processes and biotic and abiotic stress responses in animals and plants at the transcription, post-transcription, and epigenetic level, targeting various stress-responsive mRNAs, regulatory gene(s) encoding transcription factors, and numerous microRNAs (miRNAs) that regulate the expression of different genes. However, the mechanistic role of lncRNAs at the molecular level, and possible target gene(s) contributing to plant abiotic stress response and adaptation, remain largely unknown. Here, we review various types of lncRNAs found in different plant species, with a focus on understanding the complex molecular mechanisms that contribute to abiotic stress tolerance in plants. We start by discussing the biogenesis, type and function, phylogenetic relationships, and sequence conservation of lncRNAs. Next, we review the role of lncRNAs controlling various abiotic stresses, including drought, heat, cold, heavy metal toxicity, and nutrient deficiency, with relevant examples from various plant species. Lastly, we briefly discuss the various lncRNA databases and the role of bioinformatics for predicting the structural and functional annotation of novel lncRNAs.

Conclusions
Understanding the intricate molecular mechanisms of stress-responsive lncRNAs is in its infancy. The availability of a comprehensive atlas of lncRNAs across whole genomes in crop plants, coupled with a comprehensive understanding of the complex molecular mechanisms that regulate various abiotic stress responses, will enable us to use lncRNAs as potential biomarkers for tailoring abiotic stress-tolerant plants in the future.
Unlocking the relationships among population structure, plant architecture, growing season, and environmental adaptation in Henan wheat cultivars
Authors: Yang Jian , Zhou Yanjie , Hu Weiguo , Zhang Yu’e , Zhou Yong , Chen Yongxing , Wang Xicheng , Zhao Hong , Cao Tingjie , Liu Zhiyong 
Published: October 2020

AbstractBackgroundEcological environments shape plant architecture and alter the growing season, which provides the basis for wheat genetic improvement. Therefore, understanding the genetic basis of grain yield and yield-related traits in specific ecological environments is important.ResultsA structured panel of 96 elite wheat cultivars grown in the High-yield zone of Henan province in China was genotyped using an Illumina iSelect 90 K SNP assay. Selection pressure derived from ecological environments of mountain front and plain region provided the initial impetus for population divergence. This determined the dominant traits in two subpopulations (spike number and spike percentage were dominance in subpopulation 2:1; thousand-kernel weight, grain filling rate (GFR), maturity date (MD), and fertility period (FP) were dominance in subpopulation 2:2), which was also consistent with their inheritance from the donor parents. Genome wide association studies identified 107 significant SNPs for 12 yield-related traits and 10 regions were pleiotropic to multiple traits. Especially, GY was co-located with MD/FP, GFR and HD atQTL-ple5A,QTL-ple7A.1andQTL-ple7B.1region. Further selective sweep analysis revealled that regions under selection were around QTLs for these traits. Especially, grain yield (GY) is positively correlated with MD/FP and they were co-located at theVRN-1Alocus. Besides, a selective sweep signal was detected atVRN-1Blocus which was only significance to MD/FP.ConclusionsThe results indicated that extensive differential in allele frequency driven by ecological selection has shaped plant architecture and growing season during yield improvement. The QTLs for yield and yield components detected in this study probably be selectively applied in molecular breeding.
Glycaemia and hand grip strength in aging people: Guangzhou biobank cohort study
Authors: Liang Xue , Jiang Chao Qiang , Zhang Wei Sen , Zhu Feng , Jin Ya Li , Cheng Kar Keung , Lam Tai Hing , Xu Lin 
Published: October 2020

Abstract
Background
There is a link between hyperglycemia and mechanical functions of muscle. However, existing evidence of the association between hyperglycemia and weaker muscle strength is limited and inconsistent. We examined whether glycemic status was associated with relative grip strength (RGS) in older Chinese.

Methods
In 2008–2012, 9180 participants (2516 men and 6664 women) from the Guangzhou Biobank Cohort Study had fasting and 2-h post-load glucose measured. Glycemic status was categorized as normoglycaemia, prediabetes (i.e., impaired fasting glucose and/or impaired glucose tolerance) and diabetes. RGS was assessed using a Jamar Hydraulic Hand Dynamometer divided by body mass index. General linear model was used to assess the association of glycemic status with RGS.

Results
After adjusting for age, smoking status, alcohol use, physical activity, health status, body fat percentage and waist circumference, in men, hyperglycemia was associated with a lower RGS, with the RGS being 1.38 (95% confidence interval (CI) = 1.34, 1.42) in normoglycaemia, 1.35 (95% CI = 1.30, 1.39) in prediabetes, 1.33 (95% CI = 1.29, 1.38) in newly diagnosed diabetes and 1.32 (95% CI = 1.27, 1.37) in known diabetes (P for trend < 0.001). The association of glycemic status with RGS was non-significant in women. Among the normoglycaemic group, no association was found between fasting glucose and RGS in men, whereas a significantly inverse association was found in women, with adjusted β for RGS per mmol/l increase in fasting glucose being − 0.05 to − 0.04 (P values from 0.002 to 0.03).

Conclusions
Higher fasting glucose was associated with reduced grip strength in a dose-response manner, and the association was significant even in women with normoglycaemia. Our findings suggest that lowering glucose across the whole range might be important in preserving muscle strength, especially in aging women.
Feasibility of a yoga intervention to decrease pain in older women: a randomized controlled pilot study
Authors: Seguin-Fowler Rebecca , Graham Meredith , Ward Judy , Eldridge Galen , Sriram Urshila , Fine Diane 
Published: October 2020

Abstract
Background
A significant proportion of older women suffer from chronic pain, which can decrease quality of life. The objective of this pilot randomized study was to evaluate the feasibility of a flow-restorative yoga intervention designed to decrease pain and related outcomes among women aged 60 or older.

Methods
Flow-restorative yoga classes were held twice weekly for 1 hour and led by a certified yoga instructor. Participants randomized to the intervention group attended the yoga classes for 12 weeks and received supplemental materials for at-home practice. Those randomized to the control group were asked to maintain their normal daily routine. Feasibility was evaluated using recruitment and retention rates, class and home practice adherence rates, and participant satisfaction surveys. Outcome measures (self-reported pain, inflammatory markers, functional fitness, quality of life, resilience, and self-reported physical activity) were assessed at baseline and post-intervention. Paired t-tests or Wilcoxon signed-rank tests were used to examine changes in outcome measures within treatment groups.

Results
Thirty-eight participants were recruited and randomized. Participants were primarily white, college-educated, and higher functioning, despite experiencing various forms of chronic pain. Attendance and retention rates were high (91 and 97%, respectively) and the majority of participants were satisfied with the yoga program (89%) and would recommend it to others (87%). Intervention participants also experienced reductions in pain interference and improvements in energy and social functioning.

Conclusions
This pilot study provides essential data to inform a full scale randomized trial of flow-restorative yoga for older women with chronic pain. Future studies should emphasize strategies to recruit a more diverse study population, particularly older women at higher risk of disability and functional decline.

Trial registration
Clinicaltrials.gov, NCT03790098. Registered 31 December 2018 – Retrospectively registered
Implementing contact tracing for tuberculosis in Kyrgyz Republic and risk factors for positivity using QuantiFERON-TB Gold plus
Authors: Corbett Caroline , Kulzhabaeva Aizat , Toichkina Tatjana , Kalmambetova Gulmira , Ahmedov Sevim , Antonenka Uladzimir , Iskakova Altyn , Kosimova Dilorom , Migunov Dasha , Myrzaliev Bakyt , Sahalchyk Evgeni , Umetalieva Nagira , Vogel Monica , Kadyrov Abdylat , Hoffmann Harald 
Published: October 2020

Abstract
Background
Effective active case finding (ACF) activities are essential for early identification of new cases of active tuberculosis (TB) and latent TB infection (LTBI). Accurate diagnostics as well as the ability to identify contacts at high risk of infection are essential for ACF, and have not been systematically reported from Central Asia. The objective was to implement a pilot ACF program to determine the prevalence and risk factors for LTBI and active TB among contacts of individuals with TB in Kyrgyz Republic using Quantiferon-TB Gold plus (QuantiFERON).

Methods
An enhanced ACF project in the Kyrgyz Republic was implemented in which close and household (home) contacts of TB patients from the Issyk-Kul Oblast TB Center were visited at home. QuantiFERON and the tuberculin skin test (TST) alongside clinical and bacteriological examination were used to identify LTBI and active TB cases among contacts. The association for QuantiFERON positivity and risk factors were analysed and compared to TST results.

Results
Implementation of ACF with QuantiFERON involved close collaboration with the national sanitary and epidemiological services (SES) and laboratories in the Kyrgyz Republic. From 67 index cases, 296 contacts were enrolled of whom 253 had QuantiFERON or TST results; of those 103 contacts had LTBI (positive TST or IGRA), and four (1.4%) active TB cases were detected. Index case smear microscopy (OR 1.76) and high household density (OR 1.97) were significant risk factors for QuantiFERON positivity for all contacts. When stratified by age, association with smear positivity disappeared for children below 15 years. TST was not associated with any risk factor.

Conclusions
This is the first time that ACF activities have been reported for Central Asia, and provide insight for implementation of effective ACF in the region. These ACF activities using QuantiFERON led to increase in the detection of LTBI and active cases, prior to patients seeking treatment. Household density should be taken into consideration as an important risk factor for the stratification of future ACF activities.
Influence of MIF polymorphisms on CpG island hyper-methylation of CDKN2A in the patients with ulcerative colitis
Authors: Sakurai Naoko , Shibata Tomoyuki , Nakamura Masakatsu , Takano Hikaru , Hayashi Tasuku , Ota Masafumi , Nomura-Horita Tomoe , Hayashi Ranji , Shimasaki Takeo , Ostuka Toshimi , Tahara Tomomitsu , Arisawa Tomiyasu 
Published: October 2020

Abstract
Background
CDKN2A hypermethylation is among the major events associated with carcinogenesis and is also observed in non-neoplastic colonic mucosa in patients with ulcerative colitis (UC). Macrophage migration inhibitory factor (MIF) plays a crucial role in promoting gastrointestinal inflammation characteristic of UC. The aim of this study is to explore associations between CDKN2A methylation status and MIF polymorphisms (rs755622 and rs5844572).

Methods
One hundred and fifty-nine patients diagnosed with UC were enrolled in this study. The methylation status of p14ARF and p16INK4a was determined by MSP; MIF genotypes were identified by PCR-SSCP.

Results
We found no differences with respect to mean age, gender, clinical type (chronic continuous or relapse/remitting), or extent of disease among the patients with methylated and unmethylated p14ARF or p16INK4a. Carrying the rs755622 C allele indicated a significantly higher risk for p14ARF methylation (odds ratio (OR), 2.16; 95% confidence interval (CI), 1.08–4.32; p = 0.030); similarly, carrying the rs5844572 7-repeat allele indicated a significantly higher risk for p16INK4a methylation (OR, 2.57; 95% CI, 1.26–5.24; p = 0.0094) after an adjusted regression analysis. The carriers of the rs755662 C allele or the rs5844572 7-repeat allele were both at a significantly higher risk for methylation of both p14ARF and p16INK4a when compared to the cohort in which neither of the genes were methylated (OR, 2.70; 95% CI, 1.22–6.01; p = 0.015 and OR, 2.87; 95% CI, 1.25–6.62; p = 0.013, respectively). Additionally, carrying rs755622 C allele was significantly associated with CIHM in chronic continuous of clinical type and total colitis (OR, 25.9; 95% CI, 2.55–262.6; p = 0.0059 and OR, 4.38; 95% CI, 1.12–17.2; p = 0.034, respectively), and carrying 7-repeat allele of rs5844572 was significantly associated in chronic continuous type (OR, 14.5; 95%CI, 1.46–144.3; p = 0.022).

Conclusions
Taken together, our findings suggest that MIF genotypes associated with inflammation may also be involved in promoting carcinogenesis via CDKN2A hypermethylation in patients diagnosed with UC.