bluuebunny/crossref_metadata_2025_split huggingface dataset:
Flow studies on human GPVI-deficient blood under coagulating and noncoagulating conditions
Authors: Nagy Magdolna , Perrella Gina , Dalby Amanda , Becerra M. Francisca , Garcia Quintanilla Lourdes , Pike Jeremy A. , Morgan Neil V. , Gardiner Elizabeth E. , Heemskerk Johan W. M. , Azócar Lorena , Miquel Juan Francisco , Mezzano Diego , Watson Steve P. 
Published: July 2020

Abstract
The role of glycoprotein VI (GPVI) in platelets was investigated in 3 families bearing an insertion within the GP6 gene that introduces a premature stop codon prior to the transmembrane domain, leading to expression of a truncated protein in the cytoplasm devoid of the transmembrane region. Western blotting and flow cytometry of GP6hom (homozygous) platelets confirmed loss of the full protein. The level of the Fc receptor γ-chain, which associates with GPVI in the membrane, was partially reduced, but expression of other receptors and signaling proteins was not altered. Spreading of platelets on collagen and von Willebrand factor (which supports partial spreading) was abolished in GP6hom platelets, and spreading on uncoated glass was reduced. Anticoagulated whole blood flowed over immobilized collagen or a mixture of von Willebrand factor, laminin, and rhodocytin (noncollagen surface) generated stable platelet aggregates that express phosphatidylserine (PS). Both responses were blocked on the 2 surfaces in GP6hom individuals, but adhesion was not altered. Thrombin generation was partially reduced in GP6hom blood. The frequency of the GP6het (heterozygous) variant in a representative sample of the Chilean population (1212 donors) is 2.9%, indicating that there are ∼4000 GP6hom individuals in Chile. These results demonstrate that GPVI supports aggregation and PS exposure under flow on collagen and noncollagen surfaces, but not adhesion. The retention of adhesion may contribute to the mild bleeding diathesis of GP6hom patients and account for why so few of the estimated 4000 GP6hom individuals in Chile have been identified.
First-line non–anthracycline-based chemotherapy for extranodal nasal-type NK/T-cell lymphoma: a retrospective analysis from the CLCG
Authors: Qi Shu-Nan , Yang Yong , Song Yu-Qin , Wang Ying , He Xia , Hu Chen , Zhang Li-Ling , Wu Gang , Qu Bao-Lin , Qian Li-Ting , Hou Xiao-Rong , Zhang Fu-Quan , Qiao Xue-Ying , Wang Hua , Li Gao-Feng , Huang Hui-Qiang , Zhang Yu-Jing , Zhu Yuan , Cao Jian-Zhong , Wu Jun-Xin , Wu Tao , Zhu Su-Yu , Shi Mei , Xu Li-Ming , Yuan Zhi-Yong , Su Hang , Zhu Jun , Li Ye-Xiong 
Published: July 2020

Abstract
The present study investigated the survival benefit of non–anthracycline (ANT)-based vs ANT-based regimens in a large-scale, real-world cohort of patients with extranodal natural killer (NK)/T-cell lymphoma, nasal type (ENKTCL). Within the China Lymphoma Collaborative Group (CLCG) database (2000-2015), we identified 2560 newly diagnosed patients who received chemotherapy with or without radiotherapy. Propensity score matching (PSM) and multivariable analyses were used to compare overall survival (OS) and progression-free survival (PFS) between the 2 chemotherapy regimens. We explored the survival benefit of non–ANT-based regimens in patients with different treatments in early-stage disease and in risk-stratified subgroups. Non–ANT-based regimens significantly improved survivals compared with ANT-based regimens. The 5-year OS and PFS were 68.9% and 59.5% for non–ANT-based regimens compared with 57.5% and 44.5% for ANT-based regimens in the entire cohort. The clinical advantage of non–ANT-based regimens was substantial across the subgroups examined, regardless of stage and risk-stratified subgroup, and remained significant in early-stage patients who received radiotherapy. The survival benefits of non–ANT-based regimens were consistent after adjustment using multivariable and PSM analyses. These findings provide additional evidence supporting non–ANT-based regimens as a first-line treatment of patients with ENKTCL.
Development, validity and reproducibility of a whole grain food frequency questionnaire in Malaysian children
Authors: Koo H. C. , Lim G. P. , Kaur Satvinder , Chan K. Q. , Florence Tan Y. X. , Pang X. J. , Tang L. Y. 
Published: July 2020

Abstract
Background
To date, there is no validated whole grain assessment tool for children in any Southeast Asian countries. Hence, there is a need for a valid tool to assess whole grain intake among Malaysian children. This study aimed to develop, validate and test the reproducibility of a food frequency questionnaire (FFQ) in estimating whole grain intake among Malaysian children.

Methods
A total of 392 children participated in the FFQ development and 112 children aged 9–12 years participated in the validation phase; with a subsample of 50 children participating in the reproducibility phase. Three-day diet record (3DR) as the reference method in validation phase. Spearman correlations, mean difference, Bland-Altman plot and cross-classification analyses were used to assess validity. The reproducibility was tested through a repeat administration of the FFQ, with 1 month time interval. Reproducibility analyses involved intra-class correlation coefficient (ICC), Cronbach’s alpha and cross-classification analyses.

Results
The FFQ consisted of 156 whole grain food items from six food groups. Mean intake of whole grain in FFQ1 and 3DR were correlated well (r = 0.732), demonstrated good acceptance of the FFQ. Bland Altman plots showed relatively good agreement for both the dietary methods. Cross-classification of whole grain intake between the two methods showed that < 9.9% of children were grossly misclassified. Outcomes from ICC (0.989) and Cronbach’s alpha (0.995) demonstrated excellent reliability. All the children were classified in the same or adjacent quartile of whole grain intake.

Conclusions
Overall, the findings support the validity of the developed FFQ to appropriately estimate the whole grain intake in Malaysian children. This validated FFQ will be a valuable tool for future studies, to analyses the impact of whole grain consumption with disease relationship among Malaysian schoolchildren.
Bacteria pathogens drive host colonic epithelial cell promoter hypermethylation of tumor suppressor genes in colorectal cancer
Authors: Xia Xiaoxuan , Wu William Ka Kei , Wong Sunny Hei , Liu Dabin , Kwong Thomas Ngai Yeung , Nakatsu Geicho , Yan Pearlly S. , Chuang Yu-Ming , Chan Michael Wing-Yan , Coker Olabisi Oluwabukola , Chen Zigui , Yeoh Yun Kit , Zhao Liuyang , Wang Xiansong , Cheng Wing Yin , Chan Matthew Tak Vai , Chan Paul Kay Sheung , Sung Joseph Jao Yiu , Wang Maggie Haitian , Yu Jun 
Published: July 2020

Abstract
Background
Altered microbiome composition and aberrant promoter hypermethylation of tumor suppressor genes (TSGs) are two important hallmarks of colorectal cancer (CRC). Here we performed concurrent 16S rRNA gene sequencing and methyl-CpG binding domain-based capture sequencing in 33 tissue biopsies (5 normal colonic mucosa tissues, 4 pairs of adenoma and adenoma-adjacent tissues, and 10 pairs of CRC and CRC-adjacent tissues) to identify significant associations between TSG promoter hypermethylation and CRC-associated bacteria, followed by functional validation of the methylation-associated bacteria.

Results
Fusobacterium nucleatum and Hungatella hathewayi were identified as the top two methylation-regulating bacteria. Targeted analysis on bona fide TSGs revealed that H. hathewayi and Streptococcus spp. significantly correlated with CDX2 and MLH1 promoter hypermethylation, respectively. Mechanistic validation with cell-line and animal models revealed that F. nucleatum and H. hathewayi upregulated DNA methyltransferase. H. hathewayi inoculation also promoted colonic epithelial cell proliferation in germ-free and conventional mice.

Conclusion
Our integrative analysis revealed previously unknown epigenetic regulation of TSGs in host cells through inducing DNA methyltransferase by F. nucleatum and H. hathewayi, and established the latter as CRC-promoting bacteria.
Estimation of exponential growth rate and basic reproduction number of the coronavirus disease 2019 (COVID-19) in Africa
Authors: Musa Salihu S. , Zhao Shi , Wang Maggie H. , Habib Abdurrazaq G. , Mustapha Umar T. , He Daihai 
Published: July 2020

Abstract
Background
Since the first case of coronavirus disease 2019 (COVID-19) in Africa was detected on February 14, 2020, the cumulative confirmations reached 15 207 including 831 deaths by April 13, 2020. Africa has been described as one of the most vulnerable region with the COVID-19 infection during the initial phase of the outbreak, due to the fact that Africa is a great commercial partner of China and some other EU and American countries. Which result in large volume of travels by traders to the region more frequently and causing African countries face even bigger health threat during the COVID-19 pandemic. Furthermore, the fact that the control and management of COVID-19 pandemic rely heavily on a country’s health care system, and on average Africa has poor health care system which make it more vulnerable indicating a need for timely intervention to curtail the spread. In this paper, we estimate the exponential growth rate and basic reproduction number (R0) of COVID-19 in Africa to show the potential of the virus to spread, and reveal the importance of sustaining stringent health measures to control the disease in Africa.

Methods
We analyzed the initial phase of the epidemic of COVID-19 in Africa between 1 March and 13 April 2020, by using the simple exponential growth model. We examined the publicly available materials published by the WHO situation report to show the potential of COVID-19 to spread without sustaining strict health measures. The Poisson likelihood framework is adopted for data fitting and parameter estimation. We modelled the distribution of COVID-19 generation interval (GI) as Gamma distributions with a mean of 4.7 days and standard deviation of 2.9 days estimated from previous work, and compute the basic reproduction number.

Results
We estimated the exponential growth rate as 0.22 per day (95% CI: 0.20–0.24), and the basic reproduction number, R0, as 2.37 (95% CI: 2.22–2.51) based on the assumption that the exponential growth starting from 1 March 2020. With an R0 at 2.37, we quantified the instantaneous transmissibility of the outbreak by the time-varying effective reproductive number to show the potential of COVID-19 to spread across African region.

Conclusions
The initial growth of COVID-19 cases in Africa was rapid and showed large variations across countries. Our estimates should be useful in preparedness planning against further spread of the COVID-19 epidemic in Africa.
The influence of system settings on positioning accuracy in acoustic telemetry, using the YAPS algorithm
Authors: Vergeynst Jenna , Baktoft Henrik , Mouton Ans , De Mulder Tom , Nopens Ingmar , Pauwels Ine 
Published: July 2020

Abstract
Background
Acoustic positioning telemetry allows to collect large amounts of data on the movement of aquatic animals by use of autonomous receiver stations. Essential in this process is the conversion from raw signal detections to reliable positions. A new advancement in the domain is Yet Another Positioning Solver (YAPS), which combines the detection data on the receivers with a model of animal movement. This transparent, flexible and on-line available positioning algorithm overcomes problems related to traditional point-by-point positioning and filtering techniques. However, its performance has only been tested on data from one telemetry system, providing transmitters with stable burst interval. To investigate the performance of YAPS on different system parameters and settings, we conducted a simulation study.

Results
This paper discusses the effect of varying burst types, burst intervals, number of observations, reflectivity levels of the environment, levels of out-of-array positioning and temporal receiver resolution on positioning accuracy. We found that a receiver resolution better than 1 ms is required for accurate fine-scale positioning. The positioning accuracy of YAPS increases with decreasing burst intervals, especially when the number of observations is low, when reflectivity is high or when information out-of-array is used. However, when the burst interval is stable, large burst intervals (in the order of 1 to 2 min) can be chosen without strongly hampering the accuracy (although this results in information loss). With random burst intervals, the accuracy can be much improved if the random sequence is known.

Conclusions
As it turns out, the key to accurate positioning is the burst type. If a stable burst interval is not possible, the availability of the random sequence improves the positioning of random burst interval data significantly.
COVID-19 crisis, safe reopening of simulation centres and the new normal: food for thought
Authors: Ingrassia Pier Luigi , Capogna Giorgio , Diaz-Navarro Cristina , Szyld Demian , Tomola Stefania , Leon-Castelao Esther 
Published: July 2020

Abstract
Background
The world is facing a massive burden from the coronavirus disease 2019 (COVID-19) pandemic. Governments took the extraordinary step of locking down their own countries to curb the spread of the coronavirus. After weeks of severe restrictions, countries have begun to relax their strict lockdown measures. However, reopening will not be back to normal.
Simulation facilities (SF) are training spaces that enable health professionals and students to learn skills and procedures in a safe and protected environment. Today’s clinicians and students have an expectation that simulation laboratories are part of lifelong healthcare education. There is great uncertainty about how COVID-19 will impact future training in SF. In particular, the delivery of training activities will benefit of adequate safety measures implemented for all individuals involved.
This paper discusses how to safely reopen SF in the post-lockdown phase.

Main body
The paper outlines 10 focus points and provides operational tips and recommendations consistent with current international guidelines to reopen SF safely in the post-lockdown phase. Considering a variety of national advices and regulations which describe initial measures for the reopening of workplaces as well as international public health recommendations, we provide points of reflection that can guide decision-makers and SF leaders on how to develop local approaches to specific challenges. The tips have been laid out taking also into account two main factors: (a) the SF audience, mainly consisting of undergraduate and postgraduate healthcare professionals, who might face exposure to COVID-19 infection, and (b) for many simulation-based activities, such as teamwork training, adequate physical distancing cannot be maintained.

Conclusions
The planning of future activities will have to be based not only on safety but also on flexibility principles.
Sharing common methods consistent with national and international health guidelines, while taking into account the specific characteristics of the different contexts and centres, will ultimately foster dissemination of good practices.
This article seeks to further the conversation. It is our hope that this manuscript will prompt research about the impact of such mitigation procedures and measures in different countries.
Serum prolactin in patients with relapsing remitting multiple sclerosis
Authors: Alwakil Hoda A. , Al-Malt Ayman M. , Ragab Osama A. , Abdel Ghafar Muhammed T. , Tageldin Elsayed A. 
Published: July 2020

Abstract
Background
Multiple sclerosis (MS) is an inflammatory demyelinating disorder that affects the central nervous system (CNS) of females more than males. The objective of the current study was to assess serum level of prolactin (PRL) and tumor necrosis factor alpha (TNFα) in patients with relapsing remitting multiple sclerosis (RRMS) and to explore their role in disease activity.

Subjects and methods
Fifty females were included in this study, 40 patients with RRMS were evaluated during relapse and remission and 10 age-matched healthy subjects who served as controls. All patients were subjected to neurological evaluation including Expanded Disability Status Scale (EDSS), brain, and spine magnetic resonance image (MRI); serum PRL and TNFα levels were measured for all patients (during relapse and remission) and controls.

Results
Median serum PRL level was significantly higher in MS patients during relapse than remission and control subjects (P = 0.041). TNFα level was significantly higher in MS patients in relapse than remission (P = 0.026) as well as the healthy controls (P = 0.001). The area under the receiver operating characteristic curve (AUROC) was analyzed for prediction of MS relapse, AUROC of serum TNFα was 0.811 and that of serum PRL was 0.678. Both serum PRL and TNFα were positively correlated in MS patients in relapse with each other (r = 0.672, P < 0.001) and also with age, EDSS, number of relapses, and MRI lesion number (P value = 0.001).

Conclusion
Elevated serum PRL and TNFα levels are associated with relapse in MS patients. Moreover, they are positively correlated with EDSS, disease duration, and MRI lesion number.
Práticas Pedagógicas no Incentivo à Leitura e à Escrita / Pedagogical Practices to Encourage Reading and Writing
Authors: Rocha Lecenilda Barbosa , Miguel Joelson Rodrigues 
Published: July 2020

O presente estudo objetivou uma discussão sobre as Práticas Pedagógicas no Incentivo à Leitura e à Escrita. A metodologia consistiu em revisão integrativa com os seguintes aportes teóricos de autores como: Oliveira (2014), Mortatti (2016), Silva (2011), Frade (2014), orientam sobre aspectos históricos da leitura e escrita; Moreira, Vicente e Maraschin (2017), Soares (2018), Franco (2016), que discorrem sobre práticas pedagógicas de ensino da leitura e escrita; Beskow e Beskow (2015), Savage (2015), que refletem sobre os métodos de incentivo à leitura e escrita; além de autores como Fernandes (2016), Rios (2015), Nóvoa (1992). e Salomão (2014), que discutem sobre a importância da leitura e escrita para a formação do discente. Os resultados evidenciaram que cabe formalmente a escola e aos professores desenvolverem as relações entre a leitura e o indivíduo, em todas as suas interfaces. É importante ressaltar que a leitura é a base do processo de alfabetização e da formação da cidadania. Nesta perspectiva, cada professor deve ter claro que educa-se para o desenvolvimento das potencialidades do ser humano, individual e social.
Nem Sagrada, Nem Profana – Simplesmente Geni: Representações culturais no canto de Chique Buarque de Holanda / Nor Sacred, Nor Profan - Simply Geni: Cultural Representations on the music from Chique Buarque de Holanda
Authors: Purificação Marcelo Máximo , Catarino Elisângela Maura , Muller Fabiano Hector Lira , Gonçalves Ronne Clayton De Castro , Teixeira Maria Filomena Rodrigues , Paz Claudia Denis Alves da 
Published: July 2020

A personagem Geni de Chique Buarque de Holanda, aparece como uma das figuras mais emblemáticas da música popular brasileira; vista e analisada por diversas áreas do conhecimento, Geni é alvo de várias verdades e conceitos. Este trabalho, não pretende desvendar quem é Geni, mas sim, suscitar diálogos e reflexões sobre as representatividades que essa figura nos traz, vistas a partir da letra da canção em liames com o aparato teórico dos Estudos Culturais (EC).
Metodologias Ativas e Recursos Tecnológicos nos Processos de Ensino e Aprendizagem / Active Methodologies and Technological Resources in the Teaching and Learning Processes
Authors: Almeida Carlos Eugenio De Castro , Miguel Joelson Rodrigues 
Published: July 2020

Este estudo objetivou uma discussão a respeito das Metodologias Ativas e Recursos Tecnológicos nos Processos de Ensino e Aprendizagem e suas contribuições no contexto de sala de aula. Os novos recursos tecnológicos apesar de indispensáveis para a colaboração dos processos de ensino e aprendizagem, ainda representam um desafio para muitos profissionais da educação. Trata-se de uma revisão integrativa que levou em conta as idéias dos seguintes autores: Ramos (2013) a partir do momento em que a instituição escolar, os professores, a gestão participativa incluir de forma colaborativa as tecnologias com o viés de contribuição para o ensino e aprendizagem; Sancho (2006); Ramos (2012) que possibilitam visualizar a importância dos meios tecnológicos e destaca a reflexão sobre o uso dessas ferramentas; Xavier (2015) aborda as relações educador e educando e como a formação continuada oportuniza essa relação pedagógica; Campos (2011) a formação do professorado precisa contemplar novas habilidades que propiciem as ferramentas tecnológicas em espaços de mediação no processo de ensino e aprendizagem; Mendonça e Leite (2009) no tocante as metodologias ativas não são novidades, visto que têm se destacado refletindo sobre a função do docente e do educando no processo de ensino e aprendizagem; Padilha, (2018) a Metodologia Ativa é uma estratégia que põe o alunado como fundamentais agentes de seu aprendizado. Os resultados demonstraram que dominar as novas tecnologias como um instrumento que possibilite ampliar o papel ativo dos alunos, leva a um aprendizado mais significativo sobre si, sobre os outros e sobre o mundo social real. Para tanto, a capacitação docente contínua em ferramentas inovadoras são fundamentais. Neste sentido, são bem vindas políticas públicas mais eficazes em atendimento a essa demanda docente.
A Importância da Educação Ambiental para o avanço da Produção Agroecológica na Região do Semiárido Pernambucano / The Importance of Environmental Education for the Advancement of Agroecological Production in the Pernambuco Semi-Arid Region
Authors: Alencar Francisca Érica De , Campelo Junior Marcos Vinicius 
Published: July 2020

A agroecologia surgiu com o objetivo de resgatar e combinar o conhecimento do agricultor com as bases científicas, para que seja o mais sustentável possível. Na região semiárida de Pernambuco os níveis de assistência técnica são ainda muito baixos, mas alguns órgãos não governamentais realizam esses suportes. O objetivo do presente estudo foi discutir sobre a importância da educação ambiental para o avanço da produção agroecológica na região semiárida de Pernambuco. A metodologia constou de 23 estudos sobre a temática em questão, extraídos de maneira aleatória na base de dados Scielo entre Livros e artigos. Utilizou-se como filtro, as palavras-chave: Agroecologia, Agricultura Familiar e, Educação Ambiental. Os resultados levaram a crer que a expansão das práticas agroecológicas junto à educação ambiental (EA), implicaram em benefícios para a sociedade, desde o aprendizado sobre como manejar suas propriedades de forma sustentável, consumindo menos água, utilizando menos insumos externos, sem degradar o meio ambiente, bem como evitando prejudicar a saúde da sua própria família e da comunidade a que abastece com seus produtos.