bluuebunny/crossref_metadata_2025_split huggingface dataset:
Successful Laparoscopic Resection of Gastric Lymphangioma Under the Intraoperative Guidance of Indocyanine Green Fluorescence Imaging: A Case Report
Authors: Umino Ryosuke , Urabe Masayuki , Ohkura Yu , Yago Akikazu , Haruta Shusuke , Ueno Masaki , Udagawa Harushi 
Published: December 2020

Abstract
Background: Gastric lymphangioma (GLA) is an extremely rare tumor without an established therapeutic strategy. Surgical resection is considered the mainstay of treatment, although there is a high risk of local recurrence if negative margins are not achieved. Thus, it would be useful to develop tools and strategies to achieve safe and complete resection. We describe our experience with the first case involving GLA resection under the intraoperative guidance of indocyanine green (ICG) fluorescence imaging, which allowed us to achieve limited but complete resection with negative margins.Case Presentation: A 51-year-old Japanese man with a history of alcoholic liver disease underwent routine abdominal ultrasonography, which incidentally detected a 20-mm tumor adjacent to the lesser curvature of the stomach. The mass was suspected to be GLA based on its polycystic appearance. After a 16-month monitoring period, the patient was referred to our hospital for further assessment because of tumor growth and involvement of the left gastric artery. We selected surgical resection to facilitate a pathological diagnosis and treatment of potential invasion to the surrounding organs. Intraoperative use of a ICG navigation system revealed lymphatic drainage from the tumor, which we used to help determine the optimal excision line and minimize the loss of gastric volume. Pathological examination confirmed complete resection with negative margins and supported a diagnosis of lymphangioma.Conclusions: We performed laparoscopic radical resection of GLA under guidance from intraoperative ICG fluorescence imaging, which allowed us to maximize residual gastric volume. Although further cases are needed to validate this strategy, it may be useful for guiding the resection of GLA.
Blood Meal Sources and Feeding Behavior of Anophelines Mosquitoes in Bure District, Northwestern Ethiopia
Authors: Adugna Tilahun Wassie , Yewhelew Delensaw - , Degaga Emana - 
Published: December 2020

Abstract
Background: Mosquito blood meal sources determine their own feeding rates, adult survival, fecundity, hatching rates, and developmental times. The only female Anopheles mosquito takes blood meals from humans, birds, mammals, or other vertebrate animals for egg development. Studies of host preference patterns in blood-feeding anopheline mosquitoes are crucial to incriminating malaria vectors. However, the human blood index, foraging ration, and host preference index of anophelines mosquitoes were not known so far in Bure district Ethiopia.Methods: The origin of blood meals from all freshly fed and a few half-gravid exophagic and endophagic females collected using the center for disease control and prevention light trap catches were identified as human and bovine using Enzyme Linked Immune Sorbent Assay. Human blood index, forage ratio, and host feeding index were calculated.Results: A total of 617 specimens belonging to An. arabiensis (n = 209), An. funestus (n= 217), An. coustani (n= 123), An. squamosus (n= 54) and An. cinereus (n= 14) were only analyzed for blood ELISA. 577 of the overall specimens were positives for blood antigens of the host bloods. All anopheline mosquitoes assayed for blood meal sources had extremity mixed blood meals sources than single blood meals. The FR for a human was slightly > 1.0 than bovine for all Anopheles species. HFI for each pair of vertebrate hosts revealed that humans was a bit preferred blood meal source to bovine for all species (except An. squamosus), but did not exhibit a marked host selection.Conclusions: All assayed anopheline mosquitoes for blood meal ELISA had a mixed feed which tends to diminish the density of gametocytes in the mosquito stomach, thereby reducing the chance of fertilization of the female gamete and reduce the chances of malaria vector becoming infected. Moreover, An. coustani was the only species that had only human blood meal alone mean that this species has a potential to transmit the disease. Therefore, combination zooprophylaxis should be reinforced as a means of vector control because the study sites are mixed dwelling.
A Real-time Quantitative Polymerase Chain Reaction for the Specific Detection of Hammondia Hammondi and Its Differentiation from Toxoplasma Gondii
Authors: Schares Gereon , Vrhovec Majda Globokar , Tuschy Mareen , Joeres Maike , Bärwald Andrea , Koudela Bretislav , Dubey Jitender P. , Maksimov Pavlo , Conraths Franz J. 
Published: December 2020

Abstract
Introduction: Hammondia hammondi and Toxoplasma gondii are closely related protozoan parasites, but only T. gondii is zoonotic . Both species use felids as definitive hosts and cannot be differentiated by oocyst morphology. In T. gondii, a 529 bp repetitive element (TgREP-529) is of utmost diagnostic importance for PCR diagnostic tests. We identified a similar repetitive region in the H. hammondi genome (HhamREP-529).Material and Methods: Based on reported sequences, primers and probes were selected in silico and optimal primer probe combinations were explored, also by including previously published primers. The analytical sensitivity was tested using serial dilutions of oocyst DNA. For testing analytical specificity, DNA isolated from several related species were used as controls. The newly established TaqMan PCR (Hham-qPCR1) was applied to tissues collected from H. hammondi-infected gamma-interferon knockout (GKO) mice at varying time points post infection. Results: Ten forward and six reverse primers were tested in varying combinations. Four potentially suitable dual-labelled probes were selected. One set based on the primer pair (Hham275F, Hham81R) and the probe (Hham222P) yielded optimal results. In addition to excellent analytic specificity, the assay revealed an analytical sensitivity of genome equivalents of less than 1 oocyst. Investigation of the tissue distribution in GKO mice revealed the presence of parasite DNA in all examined organs, but to a varying extent suggesting 100- to 10.000-fold differences in parasitic loads between tissues in the chronic state of infection, 42 days post infection. Discussion: The use of the 529 bp repeat of H. hammondi is suitable for establishing a quantitative real-time PCR assay because this repeat probably exists about 200-times in the genome of a single organism, like its counterpart in T. gondii. Although there was enough sequence data available, only few of the primers predicted in silico revealed sufficient amplification; the identification of a suitable probe was also difficult. This is in accord with our previous observations on considerable variability in the 529 bp repetitive element of H. hammondi.Conclusions: The H. hammondi real-time PCR represents an important novel diagnostic tool for epidemiological and cell-biological studies on this and related parasites.
Do Contributing Factors for Health Inequality Really Indicate Worse Conditions Among Upper Non-manual Workers than Among Lower Non-manual and Manual Workers in Korea?: A Cross-sectional Study Using a Nationally Representative Survey Data of Korea
Authors: Noh Eunjeong , Khang Young-Ho 
Published: December 2020

Abstract
Background: Despite the consensus that higher occupational classes tend to have better health and lower mortality rates, a previous study reported reversed occupational gradients in mortality rates among Korean men after the economic crisis in the late 2000s. This study investigated occupational gradients in socioeconomic position and multiple pathway indicators known to affect mortality.Methods: We used data from 4,176 men aged 35–64 in Korea, derived from the 2007-2009 and 2013-2015 Korean National Health and Nutrition Examination Surveys, to compare the age-standardized prevalence and age-adjusted mean value of each contributing factor to health inequality among occupational groups, which were divided into upper non-manual workers, lower non-manual workers, manual workers, and others. Contributing factors included childhood and adulthood socioeconomic position indicators, biological risk factors, health behaviors, psychosocial factors, and work environment.Results: Upper non-manual workers had prominently higher levels of education, income, parental education, and economic activity than lower non-manual and manual workers. The rates of smoking and high-risk alcohol consumption were lower in the non-manual classes, and the rate of weight control activities was higher. Depression and suicidal ideation levels were lower and perceptions of the work environment were better among non-manual workers than among their manual counterparts.Conclusions: This study confirmed occupational inequality in a wide range of socioeconomic positions and pathway indicators in Korea, with consistently favorable patterns for upper non-manual workers. These occupational gradients do not support the previously reported reversed pattern of higher mortality in non-manual groups than in the manual job class in Korea.
Redistributing Ill-defined Causes of Death – A Case Study from the Burden 2020-Project in Germany
Authors: Wengler Annelene , Gruhl Heike , Plaß Dietrich , Leddin Janko , Rommel Alexander , Lippe Elena von der 
Published: December 2020

Abstract
Background The cause of death statistics in Germany include a relatively high share (26% in 2017) of ill-defined deaths (IDD). To make use of the cause of death statistics for Burden of Disease calculations we redistribute those IDD to valid causes of death.Methods The process of proportional redistribution is described in detail. It makes use of the distribution of the valid ICD-codes in the data. We use examples of stroke, diabetes, and heart failure to illustrate how IDD are reallocated. ResultsThe largest increases for both women and men can be found for lower respiratory infections, diabetes mellitus, and stroke. The numbers of deaths for these causes more than double after redistribution. ConclusionThis is the first comprehensive redistribution of IDD within the German cause of death statistics. Performing a redistribution is necessary, otherwise there would be an underreporting of certain causes of death or large numbers of deaths coded to residual or unspecific codes.
Spatial Clustering of Maternal Health Services Utilization and Its Associated Factors in Tanzania: Evidence From Tanzania Demographic Health Survey 2015-16
Authors: Assenga Blanka Thobias , Msengwa Amina Suleiman , Mbago Maurice Chakusaga 
Published: December 2020

Abstract
Background: Maternal healthcare services are major component for the health outcomes of the pregnant woman in making sure the deaths of mother and child are prohibited. This study aimed to examine the spatial clustering of maternal health care services utilization and the associated factors in Tanzania.Methods: The study utilized the Tanzania Demographic Health Survey and Malaria Indicators Survey 2015-16 data. The study carried out to 7,013 women aged between 15 and 49 years who had a live birth in the five years preceding the survey. Spatial analysis was done using the Bernoulli model through Kullodorff scan statistics, and multiple logistic regression analysis was employed to identify the predictors of maternal health services utilization Results: Spatial variations of antenatal care visit and delivery care across the regions was observed. Spatial scan statistic identified high utilization performing cluster for antenatal care centroid in Morogoro Region [RR=1.67, p<0.001] and low utilization in Kaskazini Pemba Region [RR=0.38, p<0.001]. For delivery care utilization, high utilization was observed in Mtwara Region [RR=1.83, p<0.001] and low utilization in Kigoma Region [RR=0.41, P<0.001]. Utilization of antenatal and delivery care services was higher among women from the richest households, with fewer children, living in urban areas and had acquired a higher education level. Conclusion: Results permit more insight into the differences in maternal health service utilization in relation to demographic characteristics in the country. In addition, findings highlight areas with low utilization performing clusters. Such finds are beneficial in the implementation of policy and intervention to expand maternal health care uptake in cross the regions.
Genome-wide Identification and Expression Profiling Analysis of WOX&nbsp;Family Proteins Encoded Genes in Triticeae Plant Species
Authors: Shi Lei , Wang Ke , Du Lipu , Song Yuxia , Li Huihui , Ye Xingguo 
Published: December 2020

Abstract
Background: Genotype dependence of plant regeneration is an important factor restricting the genetic improvement of Triticeae plant species. The WUSCHEL-related homeobox (WOX) is a group of plant specific transcription factor, which play an important role in plant growth and development, and cell division and differentiation. Recent studies revealed that the application of regeneration-related genes such as WOX and BABY BOOM (BBM) could improve plant regeneration. The application of WOX genes is one of the ways to improve the genetic transformation system of Triticeae and other species, but there are rare studies in this area.Results: From the available genome sequence database, in total 136 WOX transcripts were identified for the Triticeae plants, including 43 in Triticum aestivum, 30 in Triticum turgidum, 25 in Triticum dicoccoides, 17 in Hordeum vulgare, 13 in Aegilops tauschii, and 8 in Triticum urartu. All of the WOX family genes were distributed on the chromosomes of homologous groups 1 to 5 in the six Triticeae species, part of which were confirmed by their specific PCR markers using a set of T. durum-T. aestivum genome D substitution lines. All of the WOX proteins in the six Triticeae species could be grouped into three clades, similar to those in rice (Oryza sativa L.) and Arabidopsis. WOX family members were conserved among these Triticeae plants, all of them contained the conserved HOX DNA-binding homeodomain, and WUS clade members contained the characteristic WUS motif, while only TaWUS and TaWOX9 in all the six Triticeae plant species contained the ERF-associated amphiphilic repression (EAR) motif. The expression profiles of TaWOX genes by quantitative real-time PCR (qPCR) showed obvious difference among WOX family members.Conclusions: Totally 130 WOX genes were identified in the six Triticeae plant species. The WOX family genes were located on the chromosomes in the five homologous groups except groups 6 and 7 in the Triticeae species, and their expression profiles were different in different tissues, indicating that each of them had diverse function. The findings in this study could provide a basis for evolution and functional investigation and practical application of the WOX family genes in Triticeae plant species.
Analysis of Oral Microbial Dysbiosis Associated With Early Childhood Caries
Authors: Zheng Hui , Xie Teng-Fei , Li Shao-Kai , Qiao Xiao-Tong , Feng Yan , Lu You-Guang 
Published: December 2020

Abstract
Background: "Core microbes" play a key role in the development of caries and lead to microflora disorders. Our goal was to detect the core microbes associated with the microbial flora imbalance in early childhood caries (ECC). Methods: Fifteen caries-free children and fifteen high-caries (DMFT≥10) children aged 4-6 years old were recruited according to the diagnostic criteria of caries suggested by the WHO. The 16S rRNA genes from plaque samples loaded in saliva were amplified by PCR, and the PCR products were sequenced by the Illumina Miseq platform. The sequencing results were analyzed by professional software to determine the composition and structure of the saliva microorganisms.Results: There were statistically significant differences between the groups regarding the relative abundance of S. mutans (Wilcoxon rank-sum test, P<0.05). No significant difference was found between the groups regarding other species or functional genes. Conclusion: Neither unique microbes leading to early caries in deciduous teeth nor characteristics of a microbial community with a disordered structure were found. Some microorganisms related to dental caries in young children were found, but whether these microbes were truly related or were false positives requires further verification.
Random forest classification for predicting lifespan-extending chemical compounds
Authors: Kapsiani Sofia , Howlin Brendan James 
Published: December 2020

Abstract
Ageing is a major risk factor for many conditions including cancer, cardiovascular and neurodegenerative diseases. Pharmaceutical interventions that slow down ageing and delay the onset of age-related diseases are a growing research area. The aim of this study was to build a machine learning model based on the data of the DrugAge database to predict whether a chemical compound will extend the lifespan of the worm species Caenorhabditis elegans. Five predictive models were built using the random forest algorithm with molecular fingerprints and/or molecular descriptors as features. Feature selection was achieved using variation and mutual information-based methods. The best performing classifier, built using molecular descriptors, achieved an area under the curve (AUC) score of 0.815 for classifying the compounds in the test set. The features of the model were ranked using the Gini importance measure of the random forest algorithm. The top 30 most important features included descriptors related to atom and bond counts, topological and partial charge properties. The model was applied to predict the class of compounds in an external database, consisting of 1,738 small-molecules. The chemical compounds of the screening database with a predictive probability of ≥ 0.80 for increasing the lifespan of Caenorhabditis elegans were broadly separated into (i) flavonoids, (ii) fatty acids and conjugates, and (iii) organooxygen compounds.
A Personalized Nutrition Plan Improves Outcomes of Facial Regeneration With Platelet-rich Fibrin Liquid Matrixes
Authors: Nacopoulos Cleopatra , Vlastos I M , Vesala Anna-Maria , Lazou Evgenia , Chaniotis Dimitrios , Gkouskou Kalliopi 
Published: December 2020

Abstract
Background: The importance of nutrition in the prevention of skin ageing has been shown by large observational studies. Despite this fact, there are no studies assessing dietary changes as adjunct procedures to aesthetic interventions. The objective of this manuscript is to study whether a personalized nutritional plan conveys additional benefits to Platelet-Rich Fibrin (PRF) facial regenerationMethods: Forty seven healthy women (mean age 52.5 years old) were offered minimally invasive facial regeneration with the use of PRF liquid matrixes, as well as a personalized nutritional plan. The nutritional plan was informed by a nutrigenetic test based on 128 polymorphisms. Horizontal forehead lines, zygomatic wrinkles or mid-cheek furrows, nasolabial folds, perioral expression wrinkles, and marionette line were assessed separately with the use of the Facial Wrinkles Assessment Scale (FWAS)Results: Total FWAS scores change was statistically significantly better in women who reported an at least partially adaptation of nutritional recommendations for a 3-month period or longer (z=2.4, p=0.008).Conclusions: Personalized nutritional recommendations based on individual needs as well as on generally accepted dietary guidelines can improve treatment outcomes of minimally invasive facial skin aesthetics interventions.
Spinal Versus General Anesthesia for the Outpatient Unicompartmental Knee Arthroplasty: A Randomized Controlled Trial
Authors: Chen Chang , Yin Yiran , Xu Huan , Chen Ge 
Published: December 2020

Abstract
Purpose: To compare the early clinical outcomes between anesthetic techniques in outpatient unicompartmental knee arthroplasty (UKA) and investigate factors responsible for delayed discharge. Method: A randomized controlled trial was conducted on 64 consecutive and unselected patients who underwent primary, medial UKA and randomly divided into two groups:32 patients with spinal anaesthesia(Group A) and 32 with general anaesthesia(Group B). There were no significant differences in gender, age, body mass index (BMI), the hospital for Special Surgery knee score (HSS), and American Society of Anesthesiology(ASA) class between the two groups (P >0.05).Results：Totally 64 patients were involved, including 20 males and 42 females, with an average age of 60.4 ± 3.8 year-old. The length of stay(LOS) and total expense were significantly less in group A than those in group B (P < 0.05). There was no significant difference between two groups on the discharge day and the reasons for not discharge(P>0.05). Lack of mobilization (84%), pain (23%), and PONV (17%) were main reasons for patients not meeting the discharge criteria on DOS (Table 2). Patients with spinal anaesthesia underwent a longer surgery time than those with GA.(P<0.05); Patients in the Group A had a significantly earlier oral fluid intake and walking unaided(P<0.05); Pain occurred significantly earlier in Group B(P<0.05). Conclusion: In patients who underwent outpatient UKA, spinal anaesthesia is significantly superior to general anaesthesia with shorter discharge time and lower cost. With strict patient selection and safe anaesthesia, outpatient UKA could attain a good clinical outcome with both anaesthesia techniques.
Altered gray-white matter boundary in toddlers at risk for autism relates to later diagnosis of Autism Spectrum Disorder.
Authors: Godel Michel , Andrews Derek Sayre , Amaral David Gil , Ozonoff Sally , Young Gregory S. , Lee Joshua K. , Nordahl Christine Wu , Schaer Marie 
Published: December 2020

Abstract
Background: Recent neuroimaging studies have highlighted differences in cerebral maturation in individuals with autism spectrum disorder (ASD) in comparison to typical development. For instance, the sharpness of the gray-white matter boundary is decreased in adults with ASD. To determine how the gray-white matter boundary integrity relates to early ASD phenotypes, we used a regional structural MRI index called the gray-white matter contrast (GWC) on a sample of toddlers with a hereditary high risk for ASD. Methods: We used a surface-based approach to compute vertex-wise GWC in a longitudinal cohort of toddlers at high-risk for ASD imaged twice between 12 and 24 months (n=20). A full clinical assessment of ASD-related symptoms was performed in conjunction with imaging and again at three years of age for diagnostic outcome. Three outcome groups were defined (ASD, n=9; typical development, n=8; non-typical development, n=3).Results: ASD diagnostic outcome at age 3 was associated with widespread increases in GWC between age 12 and 24 months. Many cortical regions were affected, including regions implicated in social processing and language acquisition. In parallel, we found that early onset of ASD symptoms (i.e. prior to 18-months) was specifically associated with slower GWC rates of change during the second year of life. These alterations were found in areas mainly belonging to the central executive network.Limitations: Our study is the first to measure maturational changes in GWC in toddlers who developed autism, but the limited size of our sample warrants further replication in independent and larger samples. Conclusion: These results suggest that ASD is linked to early alterations of the gray-white matter boundary in widespread areas. Early onset of symptoms constitutes an independent clinical parameter associated with a specific corresponding neurobiological developmental trajectory. Altered neural migration and/or altered myelination processes potentially explain these findings.