bluuebunny/crossref_metadata_2025_split huggingface dataset:
Service Providers’ Perceptions of Support Needs for Indigenous Cancer Patients in Saskatchewan: a Needs Assessment
Authors: Sedgewick Jennifer R. , Ali Anum , Badea Andreea , Carr Tracey , Groot Gary 
Published: December 2020

Abstract
Background: In Saskatchewan, Canada, Indigenous cancer care services at the municipal, provincial, and federal levels are intended to improve quality care but can result in a complex, fragmented, and multi-jurisdictional health care system. A multi-phase needs assessment project was initiated to document Indigenous cancer care needs. Guided by Indigenous patient partners, clinicians, academics, and policy makers, the present study reflects a needs assessment of Indigenous cancer supports from the perspectives of cancer care service providers.Methods: Qualitative data were collected through three group interviews with 20 service providers for cancer patients and their families at three Saskatchewan cities. Participants included chemotherapy and radiation nurses, social workers, a patient navigator, dieticians, and practicum students. A semi-structured interview guide was used to conduct the sessions to allow for freedom of responses. Data were recorded, transcribed verbatim, and analyzed using thematic analysis. Results: Service providers’ perspectives were categorized into five themes: 1) addressing travel-related issues, 2) logistical challenges, 3) improvements to Indigenous-specific health care supports, 4) cultural sensitivity in health care, and 5) consistency in care. Supports differed for the two Indigenous groups, First Nations and Métis. Service providers made recommendations regarding how needs could be met. They saw language translation providers and Elder supports as important. Travel recommendations included medical taxis accommodating for more breaks to alleviate the uncomfortable side effects of cancer treatment and Indigenous-specific accommodations be provided. These recommendations aligned with supports that are available in four other Canadian provinces.Conclusions: These results identified gaps in supports and outlined recommendations to address barriers to cancer care from the perspectives of service providers. These recommendations may inform evidence-based health system interventions for Indigenous cancer patients and ultimately aim to improve cancer care services, quality of life, and health outcomes of Indigenous patients throughout their cancer journey.
­­STAT3 combines with Rho-ROCK signaling pathway inhibitor, regulate axon growth of ganglion cells derived from retinal Müller cells
Authors: zhou xuezhi , Wang Yujue , Peng Manjuan , He Ye , Peng Jingjie , Zhang Xuan , Wang Chao , Song Weitao 
Published: December 2020

Abstract
BackgroundMüller differentiated RGCs have potential therapeutic value for glaucoma. However, axonal regeneration of differentiated RGCs has been a difficult problem. Studies have confirmed that STAT3 and Y27632 play essential roles in regulating neuronal axon regeneration. Whether STAT3 and Y27632 can induce the Müller differentiated RGCs axon regeneration is still unknown.MethodRetina Müller cells were isolated and purified from Day 21 SD rats’ retina and were differentiated into retinal stem cells. The stem cells were randomly divided into five groups (control group, AAV-STAT3 group, shSTAT3 group, Y27632 group and AAV-STAT3 + Y27632 group). The axon length in each group were measured by ImageJ. Immunofluorescence were used to label the RGCs. The mRNA level of pluripotent associated and differentiation-associated proteins was analysed by qRT-PCR. Stem cells in different groups were injected into mice model of glaucoma. Immunohistochemical, Immunohistochemistry and OCT were performed to access RGC layer thickness in glaucoma model. VEP was used to detect the optic nerve conduction function.ResultsIn this study, we found that overexpression of STAT3 could promote the growth of RGCs axons generated by Müller cell differentiation. Combined with Y27632, axonal regeneration was significantly longer than that of the STAT3 group. However, after STAT3 was knocked out, axonal regeneration significantly decreased or even stopped. The mRNA levels of Esrrb, Prdm14, Sox2, and Rex1 in Müller differentiated RGCs after overexpression STAT3 combined with Y27632 were significantly increased, while the mRNA levels of Nestin, Eomes, Mixl1 and Gata4 were significantly decreased. The mRNA levels of Socs3, Pten, Klf9, and Mdm4 were significantly decreased, while the mRNA levels of Dclk2, Armcx1, C-MYC, and Nrn1 were significantly increased. The mRNA levels of differentiation and pluripotency marker genes showed opposite results after STAT3 deletion. After injecting Müller differentiated RGCs intervened by STAT3 combined with Y27632 into the eyes of the glaucoma model mice, the axon length, OCT displayed RGC layer thickness and the electrophysiology indicated by VEP were superior to those of the glaucoma model group.ConclusionsThese findings suggested that STAT3 combined with Y27632 can significantly improve the axonal growth level of RGCs, and reveal the potential mechanism to induce pluripotency of RGCs.
Trapped in a Vicious Cycle of Violence: A Qualitative Inquiry into the Experiences of HIV Serodiscordant Married Couples
Authors: larki mona , bahri narjes , roudsari robab latifnejad 
Published: December 2020

Abstract
Background: Violence against women is a major, complex, multidimensional and widespread public health concern worldwide. The current qualitative study was conducted to understand the experience of violence among HIV negative women in serodiscordant relationships.Methods: A qualitative description (QD) was conducted from October 2018 to January 2020 (16-month period) in Mashhad, Iran. The researchers recruited 15 HIV negative women who lived with HIV positive men through purposive sampling method. The data were collected using semi-structured interviews. Data analyzed using conventional content analysis adopted by Graneheim and Lundman (2004). Results: The main overarching theme emerged entitled: trapped in a vicious cycle of violence. It consisted of four categories of self-directed violence, intimate partner violence, cultural violence and structural violence. The cycle of violence began soon after awareness of husband's infection with acts such as suicide attempts and a sense of abhorrence for living with an infected person, and continued with confrontation with various types of violence in the family and society.Conclusions: This study provided an insight into different aspects of violence in Iranian women in HIV serodiscordant relationships. Healthcare providers should be able to address topics related to the violence for women who are in HIV serodiscordant relationships.
Community Health Workers: Challenges and Vulnerabilities of&nbsp;Accredited Social Health Activists Working in Conflict-affected Settings in the State of Assam, India
Authors: Rajbangshi Preety R , Nambiar Devaki , Srivast Aradhana 
Published: December 2020

Abstract
Introduction:. It is well acknowledged that India’s Community Health workers known as Accredited Social Health Activists (ASHA) are the bedrock of its health system. Many ASHAs are currently working in fragile and conflict-affected settings. No efforts have yet been made to understand the challenges and vulnerabilities of these female workers. This paper seeks to address this gap by bringing attention to the situation of ASHAs working in the fragile and conflict settings and how conflict impacts them and their work. Methods: Qualitative fieldwork was undertaken in four conflict-affected villages in two conflict-affected districts -Kokrajhar and Karbi Anglong of Assam state situated in the North-East region of India. Detailed account of four ASHAs from the majority (Bodo or Karbi) and minority (Adivasi or Koch) communities serving roughly 4000 people is presented. Data transliterated into English were analysed by authors by developing a codebook using grounded theory and thematic organisation of codes. Results: ASHAs reported facing challenges in ensuring access to health services during and immediately after outbreaks of conflict. They experienced difficulty in arranging transport and breakdown of services at remote health facilities. Their physical safety and security were at risk during episodes of conflict. ASHAs reported hostile attitudes of the communities they served due to the breakdown of social relations, trauma due to displacement, and loss of family members, particularly their husbands. Conclusion: Conflict must be recognised as an important context within which community health workers operate, with greater policy focus and research devoted to understanding and addressing the barriers they face as workers and as persons affected by conflict.
β-Conglycinin regulates distal intestinal immunity through the CIITA-mediated MHC II-PI3K/Akt/mTOR signaling pathway in hybrid grouper (Epinephelus fuscoguttatus ♀ × E. lanceolatus ♂): The ameliorative effects of sodium butyrate (NaB)
Authors: Yin Bin , Liu Hongyu , Tan Beiping , Dong Xiaohui , Chi Shuyan , Yang Qihui , Zhang Shuang 
Published: December 2020

Abstract
Background

β-Conglycinin (7S) is a strong immunogenic protein that modulates immune responses in the intestines of aquatic animals, whereas sodium butyrate (NaB) may promote growth by protecting the intestinal tract of fish. However, the mechanisms of action of 7S and NaB in these regards have yet to be determined.
Results

In this study, we investigated the effects of low and high doses of 7S and the ameliorative effects of NaB (based on high-dose 7S) on the growth performance, serum immunity, distal histopathology, and CIITA-mediated MHC II-PI3K/Akt/mTOR pathway in hybrid groupers (Epinephelus fuscoguttatus ♀ × E. lanceolatus ♂). The results revealed that the specific growth rate of groupers significantly increased, decreased, and increased in the low-level 7S (bL), high-level 7S (bH) high-level 7S plus NaB (bH-NaB) groups, respectively. The feed coefficient ratio was significantly increased in the bH and bH-NaB groups, whereas serum levels of IgE, IFN-γ, IL-1β, and TNF-α were upregulated in the bH group, and IgE was upregulated in the bH-NaB group. With respect to distal intestine histopathology, the intestinal diameter/plica height ratio was significantly increased in the bH group. Furthermore, there were increases in nitric oxide, nitric oxide synthase (NOS), and peroxynitrite anion (ONOO) in the bH group, and decreases in NOS and ONOO in the bH-NaB group In the distal intestinal tract, the mRNA levels of TSC1, mTOR C2, CIITA, and CREB1 were significantly upregulated in all three treatment groups, whereas those of IKKα, Rheb, mTOR C1, mLST8, EIF4B, NFY, GILT, and AEP were upregulated and downregulated in the bH and bH-NaB groups, respectively.
Conclusions

Collectively, these results indicate that 7S has a regulatory effect on serum immunity and can also affect distal intestinal tract development in hybrid groupers by modulating hindgut injury-related parameters. Within the distal intestinal tract, 7S can induce intestinal inflammation by activating the CIITA-mediated MHC II-PI3K/Akt/mTOR pathway, which eventually manifests as a reduction in growth performance. Supplementing feed with NaB represents an effective approach for enhancing serum immunity, and also protects the intestines from damage caused by high doses of 7S.
Effects of flaxseed supplementation on omega-6 to omega-3 fatty acid balance, lipid mediator profile, proinflammatory cytokines and stress indices in laying hens
Authors: Lee Sang-Mok , Kim Hee Kyum , Lee Ho-Bin , Kwon Oh-Dae , Lee Eun-Bi , Bok Jin-Duck , Cho Chong-Su , Choi Yun-Jaie , Kang Sang-Kee 
Published: December 2020

Abstract
Background: Although polyunsaturated fatty acids are in the spotlight due to their physiological effects on inflammation and stress of livestock animals, the biological roles of their derivatives, termed lipid mediators, have been little reported in laying hens. Results: In this study, two hundred 33-week-old laying hens were fed 0, 0.9, 1.8, or 3.6% (w/w) dietary flaxseed (Lintex170) with a commercial basal diet for 4 weeks to determine the physiological effects of dietary flaxseed, an omega-3-rich ingredient, on host inflammation or stress states regarding lipid mediator profiles, and also its impact on laying performance. The physiological changes in the omega-6 to omega-3 ratio, lipid mediator profiles, serum proinflammatory cytokine (tumor necrosis factor-alpha, interleukin-1beta, interleukin-6) levels, serum corticosterone levels, and the ratio of heterophils to lymphocytes were monitored. Supplementing dietary flaxseed greatly reduced the omega-6 to omega-3 fatty acid ratio from 25.85 to 4.16 in eggs and from 19.23 to 4.08 in serum samples between groups fed with 0% and 3.6% dietary flaxseed after the experimental period. In addition, the lipid mediator profiles of laying hens were modulated by supplementation with flaxseed, mainly resulting in enrichment of omega-3 fatty acid-derived lipid mediators. Furthermore, the level of proinflammatory cytokines TNF-alpha decreased when fed 3.6% (w/w) dietary flaxseed. Two stress indices, corticosterone in the serum and the heterophil to lymphocyte ratio showed significant reductions in laying hens fed 3.6% (w/w) dietary flaxseed. Additionally, overall laying performance indices were significantly improved by supplementary flaxseed. Conclusion: Taken together, these findings suggest that the decreased omega-6 to omega-3 ratio and enrichment of omega-3-derived lipid mediators induced by dietary flaxseed may contribute to reducing the stress state in laying hens, improving laying performance. These findings broaden the understanding of lipid mediator profiles in laying hens, and the results will be applied to develop antiinflammatory and antistress feed additives for the poultry industry.
Judgment grounds in surrogate decision-making in Japanese clinical practice: A qualitative survey
Authors: Tanaka Masashi , Ohnishi Kayoko , Enzo Aya , Okita Taketoshi , Asai Atsushi 
Published: December 2020

Abstract
BackgroundSurrogate decision-making is expected to become more prevalent in Japanese clinical practice. Recent years have seen an increase in activities to promote advance care planning (ACP), potentially affecting the manner in which judgments are made in surrogate decision-making. This study aimed to clarify judgment grounds on which surrogate decisions are made in Japan.MethodsThis qualitative study was based on semi-structured interviews regarding judgment grounds in surrogate decision-making for critical life-sustaining treatment choices in acute hospitals.  ResultsA total of 228 participants met the inclusion criteria, and 15 were selected for interviews. We qualitatively analyzed the content of 14 interview transcripts, excluding one which did not meet the inclusion criteria. Based on this analysis, four core categories, 17 categories, 35 subcategories, and 55 codes regarding judgement grounds in surrogate decision-making were extracted. The four core categories were as follows: Patient preference-oriented factor (Type 1); Patient interest-oriented factor (Type 2); Family preference-oriented factor (Type 3); and Balanced patient/family preference-oriented factor (Type 4). The Type 4 core category represented attempts to balance preferences of the patient with those of the surrogate decision-maker. ConclusionsSurrogate decision-makers based their decisions on important matters related to a patient’s life not only on the preferences and best interests of the patient, but also on their own preferences. As the need for surrogate decisions increase in the future, decision-makers will need to consider judgment grounds from a more diverse perspective.
Effect of adding a psychological intervention to routine care of common mental disorders in a specialized mental healthcare facility in Pakistan: a randomized controlled trial
Authors: Hamdani Syed Usman , Huma Zill-e- , Masood Aqsa , Zhou Kiana , Ahmed Zainab , Nazir Huma , Amin Hania , Akhtar Parveen , Bryant Richard , Dawson Katie , Ommeren Mark van , Wang Duolao , Rahman Atif , Minhas Fareed Aslam 
Published: December 2020

Abstract
Background: In many low resource settings, the provision of government mental health care services is limited to specialized psychiatry units in urban hospital care facilities, where the most common treatment for common mental disorders (CMDs) is pharmacotherapy, occasionally with adjunct nonspecific psychological support. We aimed to evaluate the effectiveness of adding a low intensity, psychological intervention, Problem Management Plus (PM+) for CMDs into routine care in a specialized mental health care facility in Pakistan.Methods: A two arm, single-blind individual randomized controlled trial (RCT) was carried out with adults (N=192), referred for psychological support by psychiatrists. The study participants were randomized (1:1) to PM+ plus Treatment as Usual (TAU) (n = 96) or TAU only (n = 96). The primary outcomes were symptoms of anxiety and depression, measured by the Hospital Anxiety and Depression Scale (HADS) and functional impairment as measured by WHO Disability Assessment Schedule (WHODAS 2.0) at 20 weeks after baseline.Results: The analysis was done on intention-to-treat principle. The linear mixed model analysis showed that at 20 weeks after baseline, there was a significant reduction in symptoms of anxiety and depression (mean [SD], 16.23 [8.81] vs 19.79 [7.77]; AMD, -3.10; 95% CI, −0.26 to −5.76); p=0.03 and improvement in functioning (mean [SD], 22.94 [9.37] vs 27.37 [8.36]; AMD, -4.35; 95% CI, -1.45 to -7.24); p=0.004 in PM+ plus TAU versus TAU arm . The follow-up rate was 67% at primary end-point.Conclusions: Specialized care facilities in LMICs may consider adding brief, evidence-based psychological treatments for CMDs to their routine care.Trial Registration: Australian New Zealand Clinical Trials Registry, ACTRN12616000381482. Registered March 23, 2016- Retrospectively registered, https://www.anzctr.org.au/Default.aspx/ ACTRN12616000381482
Patients and relatives as auditors of safe practices in oncology and hematology day hospitals
Authors: Rodrigo-Rincon Isabel , Aristorena Isabel Irigoyen , Tirapu-Leon Belén , Barcala Nicolas Zaballos , Carricas Maite Sarobe , Lobo-Palanco Joaquin , Caamaño Maria Luisa Antelo , Martin-Vizcaino Marta , Burnett Susan 
Published: December 2020

Abstract
BackgroundWhen there is a gap in professionals’ adherence to safe practices during cancer treatment, the consequences can be serious. Identifying these gaps in order to enable improvements in patient safety can be a challenge. This study aimed to assess if cancer patients and their relatives can be given the skills to audit reliably four safe practices, and to explore whether they are willing to play this new role.MethodsWe recruited 136 participants in 2018, from the oncology and haematology day hospital of a tertiary hospital in Spain. Patient identification, hand hygiene, blood or chemotherapy identification, and side effects related to transfusion and chemotherapy, were the safe practices selected for evaluation.The study comprised two parts: an interventional educational program and a cross-sectional design to collect data and assess to what degree participants are able and willing to be auditors depending on their characteristics using multivariate logistic regression models. A participant’s auditing skill were assessed pre and post the educational intervention.ResultsThe model was seeking predictors of being a good auditor. 63 participants (46.3%) were classified as good auditors after the training. To have younger age, higher educational level and to have had an experience of an adverse event were associated with a higher probability of being a good auditor. Additionally, 106 (77.9%) participants said that they would like to audit anonymously the professionals’ compliance of at least three of four safe practices. The willingness to audit safe practices differed depending on the safe practice but these differences did not reach statistical significance.ConclusionsThe data gathered by patients and relatives acting as auditors can provide healthcare organizations with valuable information about safety and quality of care that is not accessible otherwise. This new role provides an innovative way to engage patients and their families’ in healthcare safety where other methods have not had success. The paper sets out the methods that healthcare organizations need to undertake to enrol and train patients and relatives in an auditor role.
Regulator of cullins-1 (ROC1) negatively regulates the Gli2 regulator SUFU to activate the hedgehog pathway in bladder cancer
Authors: Wang Wei , Qiu Jianxin , Qu Pin , Chen Hui , Lan Jianyun , Chen Haitao , Li Lei , Gu Min 
Published: December 2020

Abstract
Background: The regulator of cullins-1 (ROC1) is an essential subunit in the cullin-RING ligase (CRL) protein complex and has been shown to be critical in bladder cancer cell survival and progression. This study aimed to explore the molecular mechanism of ROC1 action in the malignant progression of bladder cancer.Methods: This study utilized ex vivo, in vitro, and in vivo nude mouse experiments to assess the underlying mechanisms of ROC1 in bladder cancer cells. The expression of the components of the sonic hedgehog (SHH) pathway was determined by western blot analysis. ROC1 expression in human tumors was evaluated by immunohistochemistry.Results: ROC1 overexpression promoted the growth of bladder cancer cells, whereas knockdown of ROC1 expression had the opposite effect in bladder cancer cells. Mechanistically, ROC1 was able to target suppressor of fused homolog (SUFU) for ubiquitin-dependent degradation, allowing Gli2 release from the SUFU complex to activate the SHH pathway. Furthermore, knockdown of SUFU expression partially rescued the ROC1 knockdown-suppressed SHH activity as well as cancer cell growth inhibition. In ex vivo experiments, tissue microarray analysis of human bladder cancer specimens revealed a positive association of ROC1 expression with the SHH pathway activity. Conclusion: This study demonstrated that dysregulation of the ROC1–SUFU–GLI2 axis plays an important role in bladder cancer progression and that targeting ROC1 expression is warranted in further investigations as a novel strategy for the future control of bladder cancer.