bluuebunny/crossref_metadata_2025_split huggingface dataset:
A PhotoClick cholesterol‐based quantitative proteomics screen for cytoplasmic sterol‐binding proteins in Saccharomyces cerevisiae
Authors: Chauhan Neha , Sere Yves Y. , Sokol Anna M. , Graumann Johannes , Menon Anant K. 
Published: November 2019

AbstractErgosterol is a prominent component of the yeast plasma membrane and essential for yeast cell viability. It is synthesized in the endoplasmic reticulum and transported to the plasma membrane by nonvesicular mechanisms requiring carrier proteins. Oxysterol‐binding protein homologues and yeast StARkin proteins have been proposed to function as sterol carriers. Although many of these proteins are capable of transporting sterols between synthetic lipid vesicles in vitro, they are not essential for ergosterol transport in cells, indicating that they may be functionally redundant with each other or with additional—as yet unidentified—sterol carriers. To address this point, we hypothesized that sterol transport proteins are also sterol‐binding proteins (SBPs), and used an in vitro chemoproteomic strategy to identify all cytosolic SBPs. We generated a cytosol fraction enriched in SBPs and captured the proteins with a photoreactive clickable cholesterol analogue. Quantitative proteomics of the captured proteins identified 342 putative SBPs. Analysis of these identified proteins based on their annotated function, reported drug phenotypes, interactions with proteins regulating lipid metabolism, gene ontology, and presence of mammalian orthologues revealed a subset of 62 characterized and nine uncharacterized candidates. Five of the uncharacterized proteins play a role in maintaining plasma membrane integrity as their absence affects the ability of cells to grow in the presence of nystatin or myriocin. We anticipate that the dataset reported here will be a comprehensive resource for functional analysis of sterol‐binding/transport proteins and provide insights into novel aspects of non‐vesicular sterol trafficking.
Bioinformatic characterization of the extracellular lipases from Kluyveromyces marxianus
Authors: Martínez‐Corona Ricardo , Vázquez Marrufo Gerardo , Cortés Penagos Carlos , Madrigal‐Pérez Luis Alberto , González‐Hernández Juan Carlos 
Published: November 2019

AbstractLipases are hydrolytic enzymes that break the ester bonds of triglycerides, generating free fatty acids and glycerol. Extracellular lipase activity has been reported for the nonconventional yeast Kluyveromyces marxianus, grown in olive oil as a substrate, and the presence of at least eight putative lipases has been detected in its genome. However, to date, there is no experimental evidence on the physiological role of the putative lipases nor their structural and catalytic properties. In this study, a bioinformatic analysis of the genes of the putative lipases from K. marxianus L‐2029 was performed, particularly identifying and characterizing the extracellular expected enzymes, due to their biotechnological relevance. The amino acid sequence of 10 putative lipases, obtained by in silico translation, ranged between 389 and 773 amino acids. Two of the analysed putative proteins showed a signal peptide, 25 and 33 amino acids long for KmYJR107Wp and KmLIP3p, and a molecular weight of 44.53 and 58.23 kDa, respectively. The amino acid alignment of KmLIP3p and KmYJR107Wp with the crystallized lipases from a patatin and the YlLip2 lipase from Yarrowia lipolytica, respectively, revealed the presence of the hydrolase characteristic motifs. From the 3D models of putative extracellular K. marxianus L‐2029 lipases, the conserved pentapeptide of each was determined, being GTSMG for KmLIP3p and GHSLG for KmYJR107Wp; besides, the genes of these two enzymes (LIP3 and YJR107W) are apparently regulated by oleate response elements. The phylogenetic analysis of all K. marxianus lipases revealed evolutionary affinities with lipases from abH15.03, abH23.01, and abH23.02 families.
New $$ \mathcal{N} $$ = 2 SuperBMS3 algebra and invariant dual theory for 3D supergravity
Authors: Banerjee Nabamita , Bhattacharjee Arindam , Neetu  , Neogi Turmoli 
Published: November 2019

Abstract
We have constructed a two dimensional theory dual to 3D asymptotically flat Supergravity in presence of two supercharges with(out) internal R-symmetry. The duals in both the cases are identified with chiral Wess-Zumino-Witten models. Further gauging the theories, we show that the dual of the one without R-symmetry is invariant under the well known $$ \mathcal{N} $$
N
= 2 SuperBMS3 introduced in Banerjee et al. arXiv:1609.09210, while for the one with R-symmetry, the dual is invariant under the most generic, so far unknown, quantum $$ \mathcal{N} $$
N
= 2 SuperBMS3 symmetry. We have also commented on the phase space description of the duals.
One-loop quantum gravity from the N particle 4 spinning
Authors: Bastianelli Fiorenzo , Bonezzi Roberto , Corradini Olindo , Latini Emanuele 
Published: November 2019

Abstract
We construct a spinning particle that reproduces the propagation of the graviton on those curved backgrounds which solve the Einstein equations, with or without cosmological constant, i.e. Einstein manifolds. It is obtained by modifying the $$ \mathcal{N} $$
N
= 4 supersymmetric spinning particle by relaxing the gauging of the full SO(4) R-symmetry group to a parabolic subgroup, and selecting suitable Chern-Simons couplings on the worldline. We test it by computing the correct one-loop divergencies of quantum gravity in D = 4.
The evolution of trauma care in the Netherlands over 20 years
Authors: Hietbrink Falco , Houwert Roderick M. , van Wessem Karlijn J. P. , Simmermacher Rogier K. J. , Govaert Geertje A. M. , de Jong Mirjam B. , de Bruin Ivar G. J. , de Graaf Johan , Leenen Loek P. H. 
Published: November 2019

Abstract
Introduction
In 1999 an inclusive trauma system was initiated in the Netherlands and a nationwide trauma registry, including all admitted trauma patients to every hospital, was started. The Dutch trauma system is run by trauma surgeons who treat both the truncal (visceral) and extremity injuries (fractures).

Materials and Methods
In this comprehensive review based on previous published studies, data over the past 20 years from the central region of the Netherlands (Utrecht) was evaluated.

Results
It is demonstrated that the initiation of the trauma systems and the governance by the trauma surgeons led to a region-wide mortality reduction of 50% and a mortality reduction for the most severely injured of 75% in the level 1 trauma centre. Furthermore, major improvements were found in terms of efficiency, demonstrating the quality of the current system and its constructs such as the type of surgeon. Due to the major reduction in mortality over the past few years, the emphasis of trauma care evaluation shifts towards functional outcome of severely injured patients. For the upcoming years, centralisation of severely injured patients should also aim at the balance between skills in primary resuscitation and surgical stabilization versus longitudinal surgical involvement.

Conclusion
Further centralisation to a limited number of level 1 trauma centres in the Netherlands is necessary to consolidate experience and knowledge for the trauma surgeon. The future trauma surgeon, as specialist for injured patients, should be able to provide the vast majority of trauma care in this system. For the remaining part, intramural, regional and national collaboration is essential
Simultaneous detection and quantification of DNA and protein biomarkers in spectrum of cardiovascular diseases in a microfluidic microbead chip
Authors: Dinter Franziska , Burdukiewicz Michał , Schierack Peter , Lehmann Werner , Nestler Jörg , Dame Gregory , Rödiger Stefan 
Published: November 2019

AbstractThe rapid and simultaneous detection of DNA and protein biomarkers is necessary to detect the outbreak of a disease or to monitor a disease. For example, cardiovascular diseases are a major cause of adult mortality worldwide. We have developed a rapidly adaptable platform to assess biomarkers using a microfluidic technology. Our model mimics autoantibodies against three proteins, C-reactive protein (CRP), brain natriuretic peptide (BNP), and low-density lipoprotein (LDL). Cell-free mitochondrial DNA (cfmDNA) and DNA controls are detected via fluorescence probes. The biomarkers are covalently bound on the surface of size- (11–15 μm) and dual-color encoded microbeads and immobilized as planar layer in a microfluidic chip flow cell. Binding events of target molecules were analyzed by fluorescence measurements with a fully automatized fluorescence microscope (end-point and real-time) developed in house. The model system was optimized for buffers and immobilization strategies of the microbeads to enable the simultaneous detection of protein and DNA biomarkers. All prime target molecules (anti-CRP, anti-BNP, anti-LDL, cfmDNA) and the controls were successfully detected both in independent reactions and simultaneously. In addition, the biomarkers could also be detected in spiked human serum in a similar way as in the optimized buffer system. The detection limit specified by the manufacturer is reduced by at least a factor of five for each biomarker as a result of the antibody detection and kinetic experiments indicate that nearly 50 % of the fluorescence intensity is achieved within 7 min. For rapid data inspection, we have developed the open source software digilogger, which can be applied for data evaluation and visualization.
Simultaneous determination of perfluoroalkyl substances and bile acids in human serum using ultra-high-performance liquid chromatography–tandem mass spectrometry
Authors: Salihović Samira , Dickens Alex M. , Schoultz Ida , Fart Frida , Sinisalu Lisanna , Lindeman Tuomas , Halfvarson Jonas , Orešič Matej , Hyötyläinen Tuulia 
Published: November 2019

AbstractThere is evidence of a positive association between per- and polyfluoroalkyl substances (PFASs) and cholesterol levels in human plasma, which may be due to common reabsorption of PFASs and bile acids (BAs) in the gut. Here we report development and validation of a method that allows simultaneous, quantitative determination of PFASs and BAs in plasma, using 150 μL or 20 μL of sample. The method involves protein precipitation using 96-well plates. The instrumental analysis was performed with ultra-performance liquid chromatography–tandem mass spectrometry (UHPLC-MS), using reverse-phase chromatography, with the ion source operated in negative electrospray mode. The mass spectrometry analysis was carried out using multiple reaction monitoring mode. The method proved to be sensitive, robust, and with sufficient linear range to allow reliable determination of both PFASs and BAs. The method detection limits were between 0.01 and 0.06 ng mL−1 for PFASs and between 0.002 and 0.152 ng mL−1 for BAs, with the exception of glycochenodeoxycholic acid (0.56 ng mL−1). The PFAS measured showed excellent agreement with certified plasma PFAS concentrations in NIST SRM 1957 reference serum. The method was tested on serum samples from 20 healthy individuals. In this proof-of-concept study, we identified significant associations between plasma PFAS and BA levels, which suggests that PFAS may alter the synthesis and/or uptake of BAs.
Fewer tumour draining sentinel nodes in patients with progressing muscle invasive bladder cancer, after neoadjuvant chemotherapy and radical cystectomy
Authors: Alvaeus Julia , Rosenblatt Robert , Johansson Markus , Alamdari Farhood , Jakubczyk Tomasz , Holmström Benny , Hemdan Tammer , Huge Ylva , Aljabery Firas , Gabrielsson Susanne , Riklund Katrine , Winqvist Ola , Sherif Amir 
Published: November 2019

Abstract
Purpose
To examine the relationship between the number of tumour draining sentinel nodes (SNs) and pathoanatomical outcomes, in muscle-invasive bladder cancer (MIBC), in patients undergoing neoadjuvant chemotherapy (NAC) and radical cystectomy (RC).

Materials and Methods
In an ongoing prospective multicenter study, we included 230 patients with suspected urothelial MIBC from ten Swedish urological centers. All underwent TURb and clinical staging. From the cohort, 116 patients with urothelial MIBC; cT2-cT4aN0M0, underwent radical cystectomy (RC) and lymphadenectomy with SN-detection (SNd). 83 patients received cisplatin-based NAC and 33 were NAC-naïve. The number and locations of detected SNs and non-SNs were recorded for each patient. The NAC treated patients were categorized by pathoanatomical outcomes post-RC into three groups: complete responders (CR), stable disease (SD) and progressive disease (PD). Selected covariates with possible impact on SN-yield were tested in uni -and multivariate analyses for NAC-treated patients only.

Results
In NAC treated patients, the mean number of SNs was significantly higher in CR patients (3.3) and SD patients (3.6) compared with PD patients (1.4) (p = 0.034). In a linear multivariate regression model, the number of harvested nodes was the only independent variable that affected the number of SNs (p = 0.0004).

Conclusions
The number of tumor-draining SNs in NAC-treated patients was significantly lower in patients with progressive disease.
Laboratory investigations on the resonant feature of ‘dead water’ phenomenon
Authors: Medjdoub Karim , Jánosi Imre M. , Vincze Miklós 
Published: November 2019

Abstract
Interfacial internal wave excitation in the wake of towed ships is studied experimentally in a quasi-two-layer fluid. At a critical ‘resonant’ towing velocity, whose value depends on the structure of the vertical density profile, the amplitude of the internal wave train following the ship reaches a maximum, in unison with the development of a drag force acting on the vessel, known in the maritime literature as ‘dead water’. The amplitudes and wavelengths of the emerging internal waves are evaluated for various ship speeds, ship lengths and stratification profiles. The results are compared to linear two- and three-layer theories of freely propagating waves and lee waves. We find that despite the fact that the observed internal waves can have considerable amplitudes, linear theories can still provide a surprisingly adequate description of subcritical-to-supercritical transition and the associated amplification of internal waves. We argue that the latter can be interpreted as a coalescence of frequencies of two fundamental stable wave motions, namely lee waves and propagating interfacial wave modes.

Graphic abstract
Contribution of SST change to multidecadal global and continental surface air temperature trends between 1910 and 2013
Authors: Xu Yidan , Li Jianping , Sun Cheng , Lin Xiaopei , Liu Hailong , Wang Lanning , Liang Yishuang , Wang Qiuyun , Zhang Yazhou , Hou Zhaolu , Wang Fuchang 
Published: November 2019

AbstractThe global mean surface air temperature (GMST) shows multidecadal variability over the period of 1910–2013, with an increasing trend. This study quantifies the contribution of hemispheric surface air temperature (SAT) variations and individual ocean sea surface temperature (SST) changes to the GMST multidecadal variability for 1910–2013. At the hemispheric scale, both the Goddard Institute for Space Studies (GISS) observations and the Community Earth System Model (CESM) Community Atmosphere Model 5.3 (CAM5.3) simulation indicate that the Northern Hemisphere (NH) favors the GMST multidecadal trend during periods of accelerated warming (1910–1945, 1975–1998) and cooling (1940–1975, 2001–2013), whereas the Southern Hemisphere (SH) slows the intensity of both warming and cooling processes. The contribution of the NH SAT variation to the GMST multidecadal trend is higher than that of the SH. We conduct six experiments with different ocean SST forcing, and find that all the oceans make positive contributions to the GMST multidecadal trend during rapid warming periods. However, only the Indian, North Atlantic, and western Pacific oceans make positive contributions to the GMST multidecadal trend between 1940 and 1975, whereas only the tropical Pacific and the North Pacific SSTs contribute to the GMST multidecadal trend between 2001 and 2013. The North Atlantic and western Pacific oceans have important impacts on modulating the GMST multidecadal trend across the entire 20th century. Each ocean makes different contributions to the SAT multidecadal trend of different continents during different periods.
Early mobilization versus plaster immobilization of simple elbow dislocations: a cost analysis of the FuncSiE multicenter randomized clinical trial
Authors:   FuncSiE Trial Investigators, Van Lieshout Esther M. M. , Iordens Gijs I. T. , Polinder Suzanne , Eygendaal Denise , Verhofstad Michael H. J. , Schep Niels W. L. , Den Hartog Dennis 
Published: November 2019

Abstract
Introduction
The primary aim was to assess and compare the total costs (direct health care costs and indirect costs due to loss of production) after early mobilization versus plaster immobilization in patients with a simple elbow dislocation. It was hypothesized that early mobilization would not lead to higher direct and indirect costs.

Materials and methods
This study used data of a multicenter randomized clinical trial (FuncSiE trial). From August 25, 2009 until September 18, 2012, 100 adult patients with a simple elbow dislocation were recruited and randomized to early mobilization (immediate motion exercises; n = 48) or 3 weeks plaster immobilization (n = 52). Patients completed questionnaires on health-related quality of life [EuroQoL-5D (EQ-5D) and Short Form-36 (SF-36 PCS and SF-36 MCS)], health care use, and work absence. Follow-up was 1 year. Primary outcome were the total costs at 1 year. Analysis was by intention to treat.

Results
There were no significant differences in EQ-5D, SF-36 PCS, and SF-36 MCS between the two groups. Mean total costs per patient were €3624 in the early mobilization group versus €7072 in the plaster group (p = 0.094). Shorter work absenteeism in the early mobilization group (10 versus 18 days; p = 0.027) did not lead to significantly lower costs for loss of productivity (€1719 in the early mobilization group versus €4589; p = 0.120).

Conclusion
From a clinical and a socio-economic point of view, early mobilization should be the treatment of choice for a simple elbow dislocation. Plaster immobilization has inferior results at almost double the cost.
Investigation of the electrochemical behaviour of lead dioxide in aqueous sulfuric acid solutions by using the in situ EQCM technique
Authors: Broda Balázs , Inzelt György 
Published: November 2019

AbstractThe charge-discharge characteristics and the aging mechanism of PbO2 layers in contact with sulfuric acid solutions of different concentrations (1.5–5.0 M) were studied by using combined cyclic voltammetry and electrochemical quartz crystal microbalance (EQCM) techniques. For this purpose, thick lead dioxide layers were electrodeposited on gold substrate from aqueous solutions of Pb(NO3)2 dissolved in nitric acid. Based on the electrochemical and the mass change responses, it was found that in more concentrated solutions of H2SO4, the main reduction reaction was the transformation of lead dioxide to lead sulfate. However, in less concentrated sulfuric acid media, the transformation of lead dioxide to lead(II) ion became the main reaction. These Pb2+ ions transformed into lead sulfate crystals later by a chemical reaction. Because the electrochemical oxidation of lead sulfate is less favourable in sulfuric acid medium of higher concentrations, thus, PbO2 layers cannot be tested by continuous cyclization, which is necessary to study their aging parameters. Therefore, a delay step before each cyclic voltammogram was applied while the non-conductive lead sulfate dissolves or alternatively, by applying a pre-oxidation step prior to each cyclic voltammetry experiment to produce electrochemically significant amount of lead dioxide which can be reduced during the following negative potential sweep.
Comparing rates of atrioesophageal fistula with contact force-sensing and non-contact force-sensing catheters: analysis of post-market safety surveillance data
Authors: Calkins Hugh , Natale Andrea , Gomez Tara , Etlin Alex , Bishara Moe 
Published: November 2019

AbstractPurposeThere is limited data on the specific incidence of serious adverse events, such as atrioesophageal fistula (AEF), associated with either contact force (CF) or non-CF ablation catheters. Since the actual number of procedures performed with each type of catheter is unknown, making direct comparisons is difficult. The purpose of this study was to assess the incidence of AEF associated with the use of CF and non-CF catheters. Additionally, we aimed to understand the workflow present in confirmed AEF cases voluntarily provided by physicians.MethodsThe number of AEFs for 2014–2017 associated with each type of catheter was extracted from an ablation device manufacturer’s complaint database. Proprietary device sales data, a proxy for the total number of procedures, were used as the denominator to calculate the incidence rates. Additional survey and workflow data were systematically reviewed.ResultsBoth CF and non-CF ablation catheters have comparably low incidence of AEF (0.006 ± 0.003% and 0.005 ± 0.003%, respectively,p= 0.69). CF catheters are the catheter of choice for left atrium (LA) procedures which pose the greatest risk for AEF injury. Retrospective analysis of seven AEF cases demonstrated that high power and force and long RF duration were delivered on the posterior wall of the left atrium in all cases.ConclusionsCF and non-CF ablation catheters were found to have similar AEF incidence, despite CF catheters being the catheter of choice for LA procedures. More investigation is needed to understand the range of parameters which may create risk for AEF.
Reactive Element Effects in High-Temperature Alloys Disentangled
Authors: Babic Vedad , Geers Christine , Panas Itai 
Published: November 2019

AbstractReactive elements—REs—are decisive for the longevity of high-temperature alloys. This work joins several previous efforts to disentangle various RE effects in order to explain apparently contradicting experimental observations in alumina forming alloys. At 800–1000 °C, “messy” aluminum oxy-hydroxy-hydride transients initially formed due to oxidation by H2O which in turn undergo secondary oxidation by O2. The formation of the transient oxide becomes supported by dispersed RE oxide particles acting as water equivalents. At higher temperatures, electron conductivity in impurity states owing to oxygen vacancies in grain boundaries (GBs) becomes increasingly relevant. These channels are subsequently closed by REs pinning the said vacancies. The universality of the emerging understanding is supported by a comparative first-principles study by means of density functional theory addressing RE(III): Sc2O3, Y2O3, and La2O3, and RE(IV): TiO2, ZrO2, and HfO2, that upon reaction with water, co-decorate a generic GB model by hydroxide and RE ions. At 100% RE coverage, the GB model becomes relevant at both temperature regimes. Based on reaction enthalpy ΔHr considerations, “messy” aluminum oxy-hydroxy-hydride transients are accessed in both classes. Larger variations in ΔHr are found for RE(III)-decorated alumina GBs as compared to RE(IV). For RE(III), correlation with GB width is found, increasing with increased ionic radius. Similarly, upon varying RE(IV), minor changes in stability correlate with minor structural variations. GB decorations by Ce(III) and Ce(IV) further consolidate the emerging understanding. The findings are used to discuss experimental observations that include impact of co-doping by RE(III) and RE(IV).