bluuebunny/crossref_metadata_2025_split huggingface dataset:
Does second-look endoscopy reduce the bleeding after gastric endoscopic submucosal dissection for patients receiving antithrombotic therapy?
Authors: Uozumi Takeshi , Sumiyoshi Tetsuya , Tomita Yusuke , Tokuchi Kaho , Sakano Hiroya , Yoshida Masahiro , Fujii Ryoji , Minagawa Takeyoshi , Okagawa Yutaka , Morita Kotaro , Yane Kei , Ihara Hideyuki , Hirayama Michiaki , Kondo Hitoshi 
Published: November 2020

Abstract
Background: In patients with average risk of bleeding, second-look endoscopy does not reportedly reduce bleeding after gastric endoscopic submucosal dissection. However, effectiveness of second-look endoscopy for patients with high risk of bleeding such as those who are taking antithrombotic agents remains controversial. Hence, this study aims to clarify the effectiveness of second-look endoscopy for patients with antithrombotic therapy.Methods: We studied 142 consecutive patients with 173 gastric epithelial neoplasms who were routinely taking antithrombotic agents and were treated by endoscopic submucosal dissection at Tonan Hospital between November 2013 and December 2019. They were classified into two groups: those with second-look endoscopy (SLE group, 69 patients with 85 lesions) and those without second-look endoscopy (non-SLE group, 73 patients with 88 lesions). The incidence of post-endoscopic submucosal dissection bleeding was compared between the SLE and non-SLE groups.Results: There were no statistical differences in the rate of patients undergoing single antiplatelet therapy, single anticoagulant therapy, and multiple therapy between the SLE and non-SLE groups (SLE group vs. non-SLE group; 32 [46.4%], 16 [23.2%], and 21 [30.4%] patients vs. 37 [50.7%], 20 [27.4%], and 16 [21.9%] patients, respectively; p = 0.50). Post-endoscopic submucosal dissection bleeding incidence was 21.7% (15/69) and 21.9% (16/73) in the SLE and non-SLE groups, respectively, and did not significantly differ between the two groups (p = 0.98).Conclusions: For patients taking antithrombotic agents, the incidence of post-endoscopic submucosal dissection bleeding was not reduced by second-look endoscopy.
Effect of ARID1A mutation and expression on prognosis of hepatocellular carcinoma and cholangiocarcinoma: a meta-analysis
Authors: Meng Guangxiao , Yan Lun-Jie , Dong Zhao-Ru , Chen Zhi-Qiang , Yang Ya-Fei , Ding Zi-Niu , Li Hai-Chao , Liu Kai-Xuan , Yao Sheng-Yu , Yan Yu-Chuan , Yang Chun-Cheng , Li Tao 
Published: November 2020

Abstract
Background: Hepatocellular carcinoma (HCC) and cholangiocarcinoma are the most common liver malignant tumors worldwide. Investigating the molecular basis of these malignancies is vital to the development of new treatments. Recent studies have found that mutation and abnormal expression of ARID1A, are frequently shown in HCC and cholangiocarcinoma. Herein, we aimed to estimate the effects of ARID1A mutation and expression on prognosis of HCC and cholangiocarcinoma.Methods: We searched PubMed, EMBASE, Web of Science, the Cochrane Library for studies evaluating the relationship between ARID1A mutations or expression and the survival of HCC or cholangiocarcinoma patients. Meta-analysis was performed to generate a combined HR with a 95% confidence interval (CI) for overall survival (OS).Results: We identified 12 articles that evaluated the impact of ARID1A mutation or expression on the prognosis of patients with HCC or cholangiocarcinoma. Six studies provided data on HCC survival and 6 studies examined cholangiocarcinoma survival. For HCC, ARID1A mutation carriers or patients with low ARID1A expression had worse OS (HR = 1.75; 95% CI = 1.22–2.51) than non-carriers or patients with high ARID1A expression. For cholangiocarcinoma, ARID1A mutation carriers or patients with low ARID1A expression also had significantly shorter OS (HR = 3.70, 95% CI = 2.88–4.76).Conclusions: Our study suggests that ARIDIA mutation or low expression is strongly associated with poor prognosis of patients with HCC or cholangiocarcinoma, and may be considered as a potential prognostic biomarker for these patients.
Modulation of C5a-C5aR interactions against murine mammary cancer cell line
Authors: Kamaruddin Nurul Hazwani , Bachek Noor Farhana , Hassim Hasliza Abu , Ahmad Hafandi , Azizan Tengku Rinalfi Putra Tengku , Noor Mohd Hezmee Mohd 
Published: November 2020

Abstract
Background: Complement C5a is a potent inflammatory chemoattractant and might be a beneficial therapeutic target for the induction of an effective anti-tumour response. C5a agonist and antagonist modulation have demonstrated to have either promotes or inhibits tumour development, EMT6 murine mammary cancer cells through both in vitro and in vivo studies. Methods: For the in-vitro studies, Alamar Blue cell viability assay was used for cell viability determination and immunofluorescence assay was used to determine the location of C5aR expression on EMT6 cell line. For the in-vivo experiment, female Balb/c mice were subcutaneously injected with EMT6 tumour cells and subsequently treated with both C5aR agonist and antagonist peptides. At the end of in-vivo study period of 14 days, liver and tumour samples were obtained for an ELISA assay to quantify the levels of TNF-α, caspase-3, C5a and VEGF-A signals following the treatment with both C5aR agonist and antagonist. One-way ANOVA test was performed to evaluate the differences between the mean of treatments given in a group and that of the negative controlResults: The in-vitro experiment revealed an expression of C5aR was found on the cell membrane of the EMT6 cells and treatment with EP54; which is the C5aR agonist has shown low cell viability after 48 hours post-treatment. For the in-vivo experiment, the ELISA assay outcome have shown that EP54 significantly promote high numbers of circling of signaling proteins except for VEGF-A, suggesting that the C5aR agonist modulation might inhibits tumour development and also trigger the induction of apoptosis. Conclusion: C5aR modulation through the influence of EP54 agonism may have beneficial effects in terms of reduction of the tumour size and attenuation of its development.
Targeting EZH2-mediated methylation of histone 3 inhibits proliferation of pediatric acute monocytic leukemia cells in vitro
Authors: Ghabkari Abdulhameed , Narendran Aru 
Published: November 2020

Abstract
Background: Enhancer of zeste homolog 2 (EZH2) is a histone methyltransferase and a catalytic subunit of the polycomb repressive complex 2 (PRC2) that catalyzes the mono-, di-, and tri-methylation of histone H3 at Lys 27 (H3K27me3) to facilitate chromatin remodeling and gene silencing functions. The overexpression of EZH2 is associated with high levels of H3K27me3 in various types of cancers. Previous reports showed a significant association of EZH2 aberrations in pediatric cancers such as soft tissue sarcomas and glioblastoma. Recent reports in human subjects and animal models have also suggested a central role of EZH2 in the induction and progression of acute myeloid leukemia. Methods: In this study, we aimed to investigate the molecular status of EZH in cell lines derived from distinct pediatric leukemia to assess the efficacy of targeting EZH2 to suppress cancer cell survival and proliferation. Cell cytotoxicity and the half maximal inhibitory concentration (IC50) were measured by Alamar blue cytotoxicity assay. The molecular status of EZH2 was profiled by DNA sequencing and western blot analysis of EZH2 protein levels in THP-1, SEM, and MV4:11. In addition, Methylation of histone H3 was evaluated by immunoblotting and immunostaining analyses. Results: Our results showed that EZH2 protein is overexpressed in the pediatric monocytic cell line THP-1 , but not in other leukemia derived cell lines MV4;11 and SEM. Screening a panel of methyltransferase inhibitors revealed that three inhibitors; GSK126, UNC1999 and EPZ-5687 are the most potent inhibitors that suppressed EZH2 activity selectively on lysine 27 which resulted in increased apoptosis and inhibition of AKT and ERK protein phosphorylation in THP-1 cells. Our data demonstrated a significant increase in apoptosis in cells treated with drug combination (EZH2i and selinexor) compared to EZH2i inhibitors alone. Conclusions: Taken together, our data provide initial evidence that targeting EZH2 is a promising therapeutic strategy for the treatment of subtypes of pediatric AML. Also, combining EZH2 inhibitors with selinexor may increase the treatment efficacy in these patients. Findings from this study indicate further evaluation and consideration of these compounds for early phase clinical studies in selected pediatric malignancies in the future.
Linc00342 serves as a diagnostic and prognostic biomarker in Kidney Renal Clear Cell Carcinoma:A Comprehensive Study Based on Data mining, Clinical Analysis, and in vitro Validation
Authors: Guan Di , Liu Yuexin , Ping Hao 
Published: November 2020

Abstract
Background: Kidney renal clear cell carcinoma (KIRC), as the most common type of renal cancer, has a high mortality and recurrence rate due to the fact that many patients are in advanced stage at the time of consultation. Finding a biological marker for early-stage KIRC has become a top priority. Recently, accumulating studies have shown that lncRNA can serve as a target for diagnosis and prognosis of malignancy. However, the involvement and mechanism of linc00342 has never been researched in KIRC. The aim of this study was to investigate the diagnostic and prognostic value of linc00342 in KIRC, and to explore the effects of linc00342 on the biological functions of KIRC cells.Methods: We downloaded the linc00342 expression data and clinical information of KIRC from the TCGA database and constructed a prognostic prediction model. In vitro, the effect of knocking down linc00342 on KIRC cell proliferation, apoptosis, metastasis, and invasion was measured by colony-formation assay, flow cytometric analysis, wound-healing assay and Transwell assay, respectively.Results: Our nomogram predictive model suggested linc00342 can serve as an independent prognostic factor for KIRC. GO functional analysis and KEGG pathway analysis showed linc00342 was involved in various biological functions of KIRC, and experiments in vitro verified this. In vitro, linc00342 was overexpressed in KIRC cells as shown by RT-qPCR. Moreover, we found that linc00342 can inhibit cell apoptosis and promote cell proliferation, invasion, and migration.Conclusions: Our study is the first to examine the diagnostic and prognostic value of linc00342 in KIRC and provides new ideas for the treatment of KIRC.
Transcriptional reprogramming of the bud-mutation loquat YongLu emerging freezing resistant
Authors: Chen Fangyong , Wang Yin , Ni Haizhi , Yan Bangguo 
Published: November 2020

Abstract
Background: Freezing seriously affects loquat, an originally subtropical fruit. Here, a wide-spread cultivar loquat bud mutation ‘Yonglu’ (YL) was found to be more resistant to freezing than the parental ‘Ruantiaobaisha’ (RT), especially the fruits. The freezing resistance mechanism of YL was analyzed by physiological and transcriptomic methods. Results: After freezing treatment, only the fruits of YL showed a significant accumulation of proline (which increased by 1.5-fold). Interestingly, abscisic acid (ABA) accumulation in YL showed no difference after freezing treatment. Moreover, the stomatal density, area, and apertures of YL significantly decreased. All these results suggested that the proline content and stomatal closure contributed to the enhanced freezing tolerance. Further, the transcriptional profiles of freezing tissues were obtained and compared. Functional enrichment analysis showed that aside from basic nutrients and energy metabolisms, such as citrate cycle and oxidative phosphorylation, the fruits and flowers of YL have different systems to enhance freezing tolerance, suggesting a progressive freezing-resistant network. Signaling transduction of nearly all hormones, including ABA, was significantly differentially expressed. Several key genes, such as PP2C and SnRK2, which are crucial for transducing ABA signals, were significantly down- and upregulated, respectively. But the genes of neither ABA biosynthesis nor ABA transduction pathway significantly differentially expressed between two freezing varieties, suggesting they contribute limit in enhancing freezing tolerance of YL. Conclusion: Concludingly, the bud mutation YL loquat has stronger freezing tolerance than its parental RT due to the increasing proline and more effective regulation of stomatal closure. Furthermore, the bud mutation YL loquat reprogrammed its entire transcriptional profiles to enhance their abilities to adapt to freezing stress. However, ABA biosynthesis, ABA transduction pathway and CBF pathway, which are common reasons in cold response, were not the main reason for the increasing freezing tolerance of YL. There must be unknown regulation mechanisms for the bud mutation to freezing resistant.
Transcriptome-wide identification and characterization of microRNAs in diverse phases of wood formation in Populus trichocarpa
Authors: Wang Ruiqi , Ren Mengxuan , Tian Shuanghui , Liu Cong , Cheng He , Liu Yingying , Zhang Huaxin , Muhammad Saqib , Wei Hairong , Wei Zhigang 
Published: November 2020

Abstract
Background: MicroRNAs (miRNAs) are small, non-coding RNAs that have important regulatory functions in plant growth and development. However, the miRNAs that are involved in different developmental stages of tree stems have not been systemically characterized. In this study, we applied miRNA expression profiling method to the Populus trichocarpa trunks of the three distinct developmental stages defined as the primary stem (PS), transitional stem (TS), and secondary stem (SS) to investigate the miRNA species, their dynamic regulation and functions during the transitions of wood formation in different developmental stages at the genome-wide scale by Solexa sequencing.Results: We obtained 892, 872, and 882 known miRNAs and 1,727, 1,723, and 1,597 novel miRNAs, from PS, TS, and SS, respectively. And identified 114, 306, and 152 differentially expressed miRNAs (DE-miRNAs) with 921, 2,639, and 2,042 candidate target genes (CTGs), which formed 158, 855, and 297 DE-miRNA-CTG pairs in PS vs TS, PS vs SS, and TS vs SS , respectively. Among these, 47, 439, and 71 DE-miRNA-CTG pairs showed a significant negative correlation, respectively. Finally, we identified 39, 9, and 92 miRNA-CTG pairs involved in PS, TS, and SS, respectively. These DE-miRNA-CTG pairs in poplar or whose counterparts in other plant species are known to be transcriptional factors or structural genes involved in cell division and differentiation, cell wall modification, secondary cell wall (SCW) biosynthesis, lignification, and programmed cell death processes of wood formation. Moreover, qRT–PCR analysis confirmed that the results of small RNA-seq were robust and reliable and most miRNA-CTG pairs exhibited an inverse correlation.Conclusions: This is the first report on an integrated analysis of genome-wide mRNA and miRNA profiling of diverse phases of wood formation in poplar trunks. We showed that even though miRNAs involved in diverse developmental phases were not in a considerable number, their roles in the regulatory network that govern wood formation during different developmental stages cannot be negligible or underestimated. The information and data obtained in this paper significantly advanced our understanding of these miRNAs and their essential, dynamic and diversified roles as well as functions in diverse phases of wood formation in tree species.
Detection and Segmentation of Lesion Areas in Chest CT Scans For The Prediction of COVID-19
Authors: Ter-Sarkisov Aram 
Published: November 2020

Abstract
In this paper we compare the models for the detection and segmentation of Ground Glass Opacity and Consolidation in chest CT scans. These lesion areas are often associated both with common pneumonia and COVID-19. We train a Mask R-CNN model to segment these areas with high accuracy using three approaches: merging masks for these lesions into one, deleting the mask for Consolidation, and using both masks separately. The best model achieves the mean average precision of 44.68% using MS COCO criterion for instance segmentation across all accuracy thresholds. The classification model, COVID-CT-Mask-Net, which learns to predict the presence of COVID-19 vs common pneumonia vs control, achieves the 93.88% COVID-19 sensitivity, 95.64% overall accuracy, 95.06% common pneumonia sensitivity and 96.91% true negative rate on the COVIDx-CT test split (21192 CT scans) using a small fraction of the training data. We also analyze the effect of Non-Maximum Suppression of overlapping object predictions, both on the segmentation and classification accuracy. The full source code, models and pretrained weights are available on https://github.com/AlexTS1980/COVID-CT-Mask-Net.
Can Physical exercise be a priming adjuvant before vaccination? Insights from serological responses and severity of re-infection and reactogenicity of vaccines
Authors: AbdelMassih Antoine , Menshawey Rahma , Hozaien Rafeef , Kamel Aya , Mishriky Fady , Nathan Lauren , Yacoub Elaria , Hanoura Aia Mohamed , AlShehry Nada , Menshawey Esraa , El-Husseiny Nadine , Yasser Reem , Arsanyous Mariem , Husseiny Reem J. , Seyam Mahmoud , Massoud Doaa , Ali Nada , Kassim Assem , AmanAllah Mostafa , Elsayed Rokaya , Sheashaa Hesham , Husseiny Yousef , Hassan Nourhan , Badr Kirollos , Elkhateb Amr , Fouad Verina , Elfishawy Mayada , Medhat Omar , Mustafa Mai , Khalil Noha , Elsayed Rawan , Nada Youssef , Elshawarbi Passant , Abdelmoneim Noha , Hassan Nada , Messiha Mariam , Ghazy Marihan , Abdelfatah Emmy , Nasry Febronia , Gayed Ramy , Eesa Marian , Luis Merna , Eskandar Estfana , Yacoub Shenoda , Rajab Maram , Abdelaziz Mariam , Elgamal Nadine , Jaber Hutaf , Tayssir Sara , Michael Mark , Sabry Ahmed , Shehata Joseph , Abdelaziz Rania , Rateb Sherry , El-Maghraby Ahmed , Mahjoub Yara , Amr Alaa , Amin Amin , Kelada Peter , Saud Alaa , Ragab Shahd , Eltaher Basant , Galal Rahma Hassan , Aly Omnya Mahmoud , Aly Taquwa , AbdelHaleem Rana , ElShaarawy Areeg , Mohamed Omnia 
Published: November 2020

Abstract
Background: As more than 37 million confirmed cases and 1 million deaths worldwide from COVID-19, we flied to think about supporting immunity, So, there are more than 150 coronavirus vaccines and clinical trials are being developed. The success of those vaccines depends mainly on immunologic memory. People who are at high risk of re-infection with COVID-19 don’t show any evidence of having B-cell memory. 10-20% of infected patients don’t develop antibody response. randomized controlled trials and cross-sectional studies proved variant results about the effect of chronic exercise on vaccination function.The aim of this review is to suggest that exercise is an important adjuvant strategy for prevention of re-infection and for development of better protective responses following vaccination.Methods: Embase, Medline and the Cochrane Central Register were used to search for specific keywords such as “COVID-19” OR “SARS-CoV-2” AND “Re-infection” for relevant publications up to 1st of Nov. 2020. The systematic review was performed using PRISMA protocol.Results: According to inclusion criteria, 6 case reports from search and one case from a press conference were identified. The average age of re-infected patients was 36 years. The average intervening period between initial infection and reinfection was 105 days. 75% of cases who have been tested for antibodies after 1st infection were negative, and turned seropositive after second infection. 57% of cases developing re-infection had worse clinical manifestation.Conclusion: Regular moderate intensity exercise not only can enhance the secondary antibody response of B cell memory but also decrease the severity of re-infection and the adverse reactogenicity of potential vaccines under development.
Call your doctor: prospective description study of telemedicine during the first COVID-19 outbreak in a Swiss primary care practice
Authors: Suillot Joëlle , Zuercher Sophie , Zufferey Lydie , Sagez Julien 
Published: November 2020

Abstract
Background: Telemedicine is a healthcare assistance method which has been promoted during the COVID-19 pandemic for the management of patients. The aim of this study is to quantify the type of request that primary care physicians are experiencing, as well as the healthcare assistance modalities and ultimately, how many physical consultations are necessary.Methods: We conducted a prospective, descriptive study in a primary care practice in Switzerland on telephone consultations related to COVID-19. The data collected included the reasons for the call, symptoms, healthcare assistance modalities and follow-up. The categorical variables are expressed as percentages of the total cases or groups.Results: We included 200 calls corresponding to 113 patients. The majority of patients (76.1%) were taken care of solely through telemedicine. The physical consultations were due to the need: for a swab test (12.4%), for a somatic assessment at the practice (9.7%) and for an emergency services consultation (1.8%). In 64.6% of cases, patient assistance required only one phone call. Calls lasted 7.85 minutes on average and were more frequent on Mondays (24%) and Fridays (18.5%). Calls led to the prescription of medication in 12.5% of cases, of a bronchodilator in 3% of cases and of an antibiotic in 0.5% of cases.Conclusion: A rigorous telephone follow-up strategy carried out by primary care physicians requires few physical consultations in patients showing symptoms of COVID-19.
Evaluation of Acute Flaccid Paralysis (AFP) surveillance system, Kebbi State, Nigeria, 2013-2018
Authors: Bala Hashim Abdulmumin , Mohammed Yahaya , Barau Nyampa , Surajudeen Lukman , Ori Polycarp Uche 
Published: November 2020

Abstract
Background: Since 1988, the incidence of polio has reduced by more than 99 percent, worldwide, according to the WHO. Each year, more than 350,000 children were paralyzed, in more than 125 endemic countries. Today, only two countries are still having transmission of polio: Pakistan and Afghanistan. No wild poliovirus has been reported anywhere in Africa since 2016. A good and effective Acute Flaccid Paralysis (AFP) surveillance system is necessary to detect any suspected case of polio to interrupt the transmission of polio within any country. We evaluated the AFP surveillance system to identify gaps in its operations.Methods: We used the updated CDC guidelines for evaluating public health surveillance systems and the WHO performance standards to assess the system. We also interviewed stakeholders using an adapted questionnaire, and a key informant interview. We also reviewed AFP surveillance data from 2013-2018. We summarized data using means, frequencies, and proportion. Results: A total of 49 respondents were interviewed. Of these, 47 (98%) reported case definitions as being easy to use, and case investigation forms as being easy to fill, 46 (97%) reported data tools could easily accommodate changes, and all the surveillance officers understand the case definition of AFP. All respondents were willing to continue with the system. The non-polio AFP rate (24.6-55.2), stool adequacy (95-99.7%), timeliness, and completeness of reporting were found to be consistently above the WHO minimum standard.Conclusion: The AFP surveillance system in Kebbi State was found to be useful, simple, flexible, acceptable, sensitive, representative, timely, and stable, though donor-driven. The absence of data on 60-day follow up and feedback from the laboratory was a major problem with the system.