bluuebunny/crossref_metadata_2025_split huggingface dataset:
Activation of the NF-κB Signaling Pathway Promotes Malignancy in Bladder Cancer Cells in a Positive Feedback Manner
Authors: guan Zhenfeng , Sun Yi , Jiang YaZhuo , Wang Xinyang , Fan Jinhai 
Published: August 2020

Abstract
Background: The main issue arising from bladder cancer (BCa) is the high relapse ratio and tumor progression, the mechanism of which remains to be elucidated. Interaction of tumor cells with the stroma of microenvironment promoting tumor progression warrants much attention from researchers. Among all stromal cells, endothelial cells (ECs) are exceptional. Numerous studies have investigated its role of angiogenesis, but have not studied immunocyte recruitment and chemokine secretion, the important significance of which in tumor progression has been proven. Meanwhile, to the best of our knowledge, few studies have focused on the direct interaction between tumor cells and ECs in BCa tissue, which was the aim of the present study. Methods: In the present study, immunohistochemical staining is used for detecting the distribution of ECs in BCa tissue, and we use SPSS 19 to analysis the relationship between ECs distribution and tumor grade/stage; inadition, co-curlturing of tumor cell with ECs is usd to mimicking the interaction of tumor cell with ECs, followed by Chamber Assay, BrdU incorporoartion, WB, Qt-PCR, ect, to investiatin the mechanism. Results: The distribution of ECs in BCa tissue is significantly increased according to BCa grade and negatively associated to the time from BCa diagnosis to progression, manifesting as an independent risk factor for BCa prognosis. The following in vitro experiment indicates that the conditional medium from co-culture of tumor cells (T24/J82) with ECs (human umbilical vein endothelial cells, which were used as ECs in the in vitro experiment) contributes to the activation of the NF-ĸB signaling pathway in tumor cells, leading to the upregulation of CXCL1/8. This further results in enhanced tumor cell malignancy and EC recruitment, manifested as a positive feedback loop. Conclusions: The present study provided a further understanding on the role of ECs in BCa progression—not only by angiogenesis but also by interacting with tumor cell dirctly.
Immune Gene Prognostic Signature for Disease Free Survival of Gastric Cancer: Translational Research of an Artificial Intelligence Survival Predictive System
Authors: Zhang Zhiqiao , He Tingshan , Huang Liwen , Li Jing , Wang Peng 
Published: August 2020

Abstract
Background: The progress of artificial intelligence algorithms and massive data provide new ideas and choices for individual mortality risk prediction for cancer patients. The current research focused on depict immune gene related regulatory network and develop an artificial intelligence survival predictive system for disease free survival of gastric cancer. Methods: Multi-task logistic regression algorithm, Cox survival regression algorithm, and Random survival forest algorithm were used to develop the artificial intelligence survival predictive system.Results: Nineteen transcription factors and seventy immune genes were identified to construct a transcription factor regulatory network of immune genes. Multivariate Cox regression identified fourteen immune genes as prognostic markers. These immune genes were used to construct a prognostic signature for gastric cancer. Concordance indexes were 0.800, 0.809, and 0.856 for 1-, 3- and 5- year survival. An interesting artificial intelligence survival predictive system was developed based on three artificial intelligence algorithms for gastric cancer. Gastric cancer patients with high risk score have poor survival than patients with low risk score.Conclusions: The current study constructed a transcription factor regulatory network and developed two artificial intelligence survival prediction tools for disease free survival of gastric cancer patients. These artificial intelligence survival prediction tools are helpful for individualized treatment decision.
Dual species dynamic transcripts reveal the interaction mechanisms between&nbsp;Chrysanthemum morifolium&nbsp;and&nbsp;Alternaria alternata
Authors: Liu Lina , Chen Fadi , Chen Sumei , Fang Weimin , Liu Ye , Guan Zhiyong 
Published: August 2020

Abstract
Background
Chrysanthemum (C. morifolium) black spot disease caused by Alternaria alternata is one of the plant’s most destructive diseases. Dual RNA-seq was performed to simultaneously assess their transcriptomes to analyze the potential interaction mechanism between the two species, i.e., host and pathogen.
Results
C. morifolium and A. alternata were subjected to dual RNA-seq at 1, 12, and 24 hours after inoculation, and differential expression genes (DEGs) in both species were identified. This analysis confirmed 153,532 DEGs in chrysanthemum and 14,932 DEGs in A. alternata, that were involved in plant-fungal interactions and phytohormone signaling. Fungal DEGs such as toxin synthesis related enzyme and cell wall degrading enzyme genes played important roles during chrysanthemum infecton. Moreover, a series of key genes highly correlated with the early, middle, or late infection stage was identified, together with the regulatory network of key genes annotated in PRG or PPI databases. Highly correlated genes were identified at the late infection stage, expanding our understanding of the interplay between C. morifolium and A. alternata. Additionally, six DEGs each from chrysanthemum and A. alternata were selected for qRT-PCR assays to validate the RNA-seq output.
Conclusions
Collectively, data obtained in this study enriches the resources available for research into the interactions that exist between chrysanthemum and A. alternata, thereby providing a theoretical basis for the development of new chrysanthemum varieties with resistance to pathogen.
Are activated B cells involved in the process of myocardial fibrosis after acute myocardial infarction? -An in vivo experiment
Authors: Mo Fanrui , Luo Ying , Yan Yuluan , Li Juan , Lai Shayi , Wu Weifeng 
Published: August 2020

Abstract
Background: Inflammatory cells infiltrate into the ischemic and hypoxic myocardial tissue after myocardial infarction. B cells gather at the site of myocardial injury and secrete cytokines to regulate immune inflammation and fiber repair processes.Methods: The animal experiment intended to use ligation of the left anterior descending branch of C57BL/6 mice to establish a mice AMI model to observe the changes of activated B cells and cytokines at different time points. 88 12-week-old C57BL/6 male mice were divided into the Sham group (24 mice) and acute myocardial infarction (AMI ) group (64 mice) randomly. Besides, C57BL/6 Bmi-1 knock out (BKO) mice and C57BL/6 wild-type (WT) mice were used to establish AMI models to observe the expression levels of cardiomyocyte factors including TNF-α and IL-1β, IL-6, TGF-β1, COL1-A1, COL3-A1, TIMP-1, and MMP9. Moreover, pathological and collagen changes in the myocardium were analyzed. One-way ANOVA analysis of variance was used for comparison between multiple groups, and the LSD method was used for pairwise comparison between groups. P < 0.05 indicated statistical differences.Results: AMI model of C57BL/6 mice was established successfully. The ratio of activated B cells and expression of TNF-α, IL-1β, IL-6, TGF-β1, and BAFF in 5 days subgroup was highest in the myocardium, spleen, and peripheral blood with the most obvious myocardial inflammatory cells infiltration. mRNA expression levels of TNF-α, IL-1β, IL-6, TGF-β1 in 5 days subgroup of the BKO group were decreased compared with the WT group (P < 0.05). Among 2 weeks subgroups of Sham, WT, and BKO groups, the LVEDd and LVESd in the BKO and WT groups were less than those in the Sham group (P < 0.05). The LVEDd and LVESd of the BKO group were less than the WT group,EF was higher than the WT group (P < 0.05).Conclusion: Activated B cells participated in the sustained state of myocardial inflammation and immune system activation after AMI via promoting the secretion of cytokines, and may affect the metabolism of myocardial collagen. Moreover, B cells could damage the heart structure and left ventricular ejection function by promoting the expression of myocardial collagen Type I and type III.
Validation of the Family Health Behavior Scale for the Brazilian Population
Authors: Preto Luiza T. , Scarpatto Camila H. , Ley Laura Lessa Gaudie , Silveira Carolina , Salerno Margareth Rodrigues , Moreno Jennette Palcic , Castro Stela Maris de Jezus , Booij Linda , Mattiello Rita 
Published: August 2020

Abstract
Background: It is well known that the family environment is associated with child health behavior and outcomes. However, there is a lack of available instruments to measure family health behaviors, even more so in languages other than English. The aim of the present study was to adapt and validate the Family Health Behavior Scale (FHBS) for Brazilian families.Methods: The FHBS was translated and culturally adapted for the Brazilian families. Psychometric properties (content validity, construct validity, and concurrent validity) and reliability (internal consistency, ceiling-floor effect, and test-retest) were evaluated in a sample of healthy community-dwelling children who were between 5 to 12 years old, of both sexes. Caregivers responded to the FHBS. Concurrent validity was assessed by comparing the FHBS scale with Body Mass Index (BMI) percentile, percent body fat, and physical activity level.Results: 272 children (54% girls) with a mean age of 7.9 years (SD = 2.0) and their caregivers were tested. Confirmatory analysis of the initial 4-factor structure (as proposed in the original English version of the questionnaire) suggested its performance was below acceptable. Exploratory factor analysis showed however an acceptable fit (Kaiser-Meyer-Olkin index = 0.79), and the factor loadings suggested a 7-factor model. Children who were considered obese (BMI percentile ≥ 97) had lower mean total FHBS scores than children who had a healthy weight or were overweight. Children who were classified as having a healthy fat percentage had higher FHBS scores than children classified as having an excessively high fat percentage. Children who were physically active had significantly higher total FHBS scores than children who were physically inactive. Cronbach's alpha was 0.81 and we noted acceptable values of the ceiling-floor effect. Test-retest analyses showed lower agreement and intraclass correlation coefficients ​​than expected 0.63 (95% CI 0.41 to 0.78).Conclusion: The adapted Family Health Behavior Scale for the Brazilian population showed adequate psychometric properties.
An Open Label, Pilot Study of Veliparib and Lapatinib in Patients with Metastatic, Triple Negative Breast Cancer
Authors: Stringer-Reasor Erica M. , May Jori E. , Olariu Eva , Caterinicchia Valerie , Li Yufeng , Manna Deborah L. Della , Rocque Gabrielle B. , Vaklavas Christos , Falkson Carla I. , Nabell Lisle M. , Acosta Edward P. , Forero-Torres Andres , Yang Eddy S. 
Published: August 2020

Abstract
Background: Poly (ADP-ribose)-polymerase inhibitors (PARPi) have been approved for cancer patients with germline BRCA1/2 (g BRCA1/2 ) mutations, and efforts to expand the utility of PARPi beyond BRCA1/2 are ongoing. In preclinical models of triple negative breast cancer (TNBC) with intact DNA repair, we previously showed an induced synthetic lethality with combined EGFR inhibition and PARPi. We report the safety and clinical activity of lapatinib and veliparib in patients with metastatic TNBC. Methods: A first-in-human, pilot study of lapatinib and veliparib was conducted in metastatic TNBC (NCT02158507). The primary endpoint was safety and tolerability. Secondary endpoints were objective response rates and pharmacokinetic evaluation. Gene expression analysis of pre-treatment tumor biopsies was performed. Key eligibility included TNBC patients with measurable disease and prior anthracycline and taxane therapy. Patients with g BRCA1/2 mutations were excluded. Results: Twenty patients were enrolled of which 17 were evaluable for response. Median number of prior therapies in the metastatic setting was 1 (range 0-2). Fifty percent of patients were Caucasian, 45% African-American, and 5% Hispanic. Of evaluable patients, 4 demonstrated a partial response and 2 had stable disease. There were no dose-limiting toxicities. Most AEs were limited to grade 1 or 2 and no drug-drug interactions noted. Gene expression analysis suggest baseline DNA repair pathway score was lower and baseline immunogenicity was higher in the responders compared to non-responders. Conclusions: Lapatinib plus veliparib therapy has a manageable safety profile and promising antitumor activity in advanced TNBC. Further investigation of dual therapy with EGFR inhibition and PARP inhibition is needed.
Associations Between Developmental Timing of Child Abuse and Conduct Problem Trajectories
Authors: Bauer Andreas , Hammerton Gemma , Fraser Abigail , Fairchild Graeme , Halligan Sarah L. 
Published: August 2020

Abstract
Background Although there is strong evidence for a relationship between child maltreatment and conduct problems, associations between child abuse experienced at different developmental stages and developmental trajectories of conduct problems have not been examined. We sought to investigate effects of timing of child abuse on conduct problem trajectories in a large UK-based birth cohort study. Methods We applied latent class growth analysis to identify conduct problem trajectories in the Avon Longitudinal Study of Parents and Children, using parent-rated conduct problems from ages 4-17 years (N=10648). Childhood-only and adolescence-only abuse, in addition to abuse in both developmental periods (‘persistent’ abuse), were assessed by retrospective self-report at age 22 years (N=3345). Results We identified four developmental trajectories: early-onset persistent (4.8%), adolescence-onset (4.5%), childhood-limited (15.4%), and low (75.3%) conduct problems. Childhood-only abuse and ‘persistent’ abuse were associated with increased odds of being on the early-onset persistent and adolescence-onset conduct problem trajectories compared to the low conduct problems trajectory. Adolescence-only abuse was not predictive of trajectory membership. There were no associations between abuse and childhood-limited trajectory membership.Conclusions Early-onset persistent and adolescence-onset conduct problems showed similar patterns of associations with abuse exposure, challenging developmental theories that propose qualitative, as opposed to quantitative, differences in environmental risk factors between these trajectories. The results also highlight that childhood-only and ‘persistent’ abuse were more strongly linked to elevated conduct problem trajectories than adolescence-only abuse, and that ‘persistent’ abuse is particularly detrimental.
Peritumoral Plasmacytoid Dendritic Cells Predict a Poor Prognosis for Intrahepatic Cholangiocarcinoma After Curative Resection
Authors: Hu Zhi-Qiang , Zhou Zheng-Jun , Luo Chu-Bin , Xin Hao-Yang , Li Jia , Yu Song-Yang , Zhou Shao-Lai 
Published: August 2020

Abstract
Background
Plasmacytoid dendritic cells (pDCs) are present in various primary and metastatic human neoplasms; however, their clinical significance in intrahepatic cholangiocarcinoma is not clear.
Methods
To evaluate pDCs’ distributions in and around tumors as well as their potential function and predictive value for prognosis in patients undergoing curative resection, we performed immunohistochemistry to examine the expression of pDC marker BDCA2, and CD3, CD4, CD8 and Foxp3 in intratumoral and peritumoral tissues from 359 patients with intrahepatic cholangiocarcinoma and compared with prognostic and clinicopathologic factors.
Results
Results showed that patients with high numbers of BDCA2+ pDCs in peritumoral tissues were more likely to have elevated levels of carbohydrate antigen 19 − 9 and gamma-glutamyl transferase, larger and more tumors, advanced tumor-node-metastasis staging, more vascular/bile duct invasion, and lymphatic metastasis in association with greater chance of recurrence and shorter overall survival. Peritumoral tissues with larger numbers of pDCs also showed increased Foxp3+ regulatory T cell infiltration, both of which were found to be independent factors for predicting time to recurrence and overall survival. By contrast, patient outcomes were not associated with the presence of intratumoral pDCs.
Conclusions
Peritumoral pDC infiltration may indicate an immune tolerogenic peritumor microenvironment and can be used to predict a poor prognosis for patients undergoing curative resection for intrahepatic cholangiocarcinoma.
Landscape of Cell Heterogeneity and Evolutionary Trajectory in Ulcerative Colitis-associated Colon Cancer Revealed by Single-cell Rna Sequencing
Authors: Wang Quan , Wang Zhu , Zhang Zhen , Zhang Wei , Zhang Mengmeng , Shen Zhanlong , Ye Yingjiang , Jiang Kewei , Wang Shan 
Published: August 2020

Abstract
Background: Patients with colitis-associated cancer (CAC), a particular kind of colorectal cancer that develops from inflammatory bowel diseases (IBDs), have an earlier morbidity and a poorer prognosis. However, in CAC, single cell transcriptome analysis of the microenvironment composition and characteristics has yet to be performed. To understand the intra-tumor heterogeneity in CAC and to reveal a potential evolutionary trajectory from ulcerative colitis (UC) to CAC at the single cell level. Methods: Fresh samples of tumor- and adjacent tissue, from a CAC patient with pT3N1M0, were examined by single cell RNA sequencing (scRNA-seq). Data from The Cancer Genome Atlas (TCGA) and The Human Protein Atlas were used to confirm the different expression levels in normal and tumor tissues and to determine their relationships with prognosis. Results: Ultimately, 4,777 single-cell transcriptomes (1220 genes per cell) were studied, which composed of 2,250 (47%) and 2,527(53%) originated from tumor- and non-malignant tissue, respectively. And we defined the composition of cancer-associated stromal cells and identified six cell clusters included myeloid, T and B cells, fibroblasts, endothelial and epithelial cells. Likewise, the notable pathways and transcription factors (TFs) involved of these cell clusters were analyzed and described. Moreover, we graphed the precise cellular composition and developmental trajectory from UC to UC-associated colon cancer, and predicted that CD74, CLCA1 and DPEP1 had a potential role in the disease progression. Conclusions: scRNA-seq technology could reveal intratumor cell heterogeneity in ulcerative colitis-associated colon cancer, and might provide a promising direction to seek the novel potential therapeutic targets in the evolution from IBD to CAC.
Long Non-Coding RNA LINC00663 Promotes Gliomagenesis and Progression
Authors: Pan Meichen , Shi Jingren , Yin Shangqi , Meng Huan , He Chaonan , Wang Yajie 
Published: August 2020

Abstract
Background: Glioma is the most frequent primary malignant brain tumor, characterized by high morbidity, high mortality and dismal prognosis. Numerous analyses have revealed the abnormal expression of long non-coding RNAs (lncRNAs) in glioma cells. This study aims to explore its role in glioma development and prognosis.Methods: The gene expression in cell lines were measured by qRT-PCR. The role of LINC00663 in glioma was confirmed by CCK8, EdU assay, transwell and western blot as well as by in vivo experiments. Besides, Pearson's correlation analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis were also performed as needed.Results: Firstly, data from our preliminary work (NSFC NO. 81572474) showed that LINC00663 might be largely implicated in glioma. Meanwhile, LINC00663 upregulation confirmed in glioma predicted poor clinical outcomes. Functionally, LINC00663 knockdown restrained cell proliferation, migration and invasion in vitro. Mechanistic investigations validated that LINC00663 silencing decreased AKT activation in glioma cells.Conclusions: LINC00663 promotes glioma development and progression through regulating AKT pathway, suggesting LINC00663 as a probable target for glioma treatment.
Retrospective Analysis of Paraspinal Muscle-Splitting Microscopic-Assisted Discectomy Versus Percutaneous Endoscopic Lumbar Discectomy on Patients in the Treatment of Far-Lateral Lumbar Disc Herniation
Authors: Kong Lei , Zhang Wei-Zhi , Xu Hong-Guang 
Published: August 2020

Abstract
Background: Minimally invasive surgery includes percutaneous endoscopic lumbar discectomy and the microscopic tubular technique. This study aimed to compare the two techniques and evaluate the outcomes of the procedures.Methods: We retrospectively analyzed patients with far-lateral lumbar disc herniation (FLLDH) from June 2015 to October 2018. Twenty-six patients underwent paraspinal muscle-splitting microscopic-assisted discectomy (MD) and 30 patients underwent percutaneous endoscopic lumbar discectomy (PELD) surgery by the same surgical team. Data included the duration of the operation, duration of intraoperative radiation exposure, and average duration of hospitalization. Pre- and postoperative pain scores and neurological functions were recorded using a visual analog scale (VAS) score and Oswestry disability index (ODI).Results: 56 patients remained in the study over the 12–24 months period. The mean operating time was 65.83 ± 16.64 min in the PELD group, mean duration of radiation exposure was 2.87 ± 1.19 min, and average of hospitalization was 3.43 days. The mean operating time was 44.96 ± 16.87 min in the MD group, duration of radiation exposure was 0.78 ± 0.32 min, and duration of hospitalization was 4.12 days. There were two patients with postoperative transient dysesthesia and one underwent reoperation 7 months after surgery in the PELD group. One patient had postoperative transient dysesthesia in the MD group. Except low back pain at 3 months (p >0.05), all patients in both groups showed significant improvement in VAS and ODI scores compared with pre-operation and until final follow-up (p<0.05). Although the learning curve of MD is shorter compared with the PELD, beginners should practice on cadavers and receive teaching demonstrations from senior surgeons.Conclusion: Both techniques are minimally invasive, effective, and safe for treating far-lateral lumbar disc herniation in selected patients. Compared with the PELD technique, the MD procedure offers a wider field of vision during operation, shorter operation time, fewer postoperative complications, and shorter learning curve.
Oral Cenesthopathy Proceeding Dementia With Lewy Body: A Case Report
Authors: Sawada Yuta , Konishi Yuki , Ikenouchi Atsuko , Yoshimura Reiji 
Published: August 2020

Abstract
Somatic hallucination is defined as the false sensation of an occurrence in the body. When the hallucination is grotesque and visceral, it is classified as a cenesthopathy (1). Cenesthopathy is likely influenced by culture; one study reported that, of the seven unique countries from which patients were recruited, those from Ghana and those with chronic schizophrenia were significantly more likely to report cenesthesia (2).