bluuebunny/crossref_metadata_2025_split huggingface dataset:
In situ-forming collagen hydrogel crosslinked via multi-functional PEG as a matrix therapy for corneal defects
Authors: Fernandes-Cunha Gabriella Maria , Chen Karen Mei , Chen Fang , Le Peter , Han Ju Hee , Mahajan Leela Ann , Lee Hyun Jong , Na Kyung Sun , Myung David 
Published: October 2020

AbstractVisually significant corneal injuries and subsequent scarring collectively represent a major global human health challenge, affecting millions of people worldwide. Unfortunately, less than 2% of patients who could benefit from a sight-restoring corneal transplant have access to cadaveric donor corneal tissue. Thus, there is a critical need for new ways to repair corneal defects that drive proper epithelialization and stromal remodeling of the wounded area without the need for cadeveric donor corneas. Emerging therapies to replace the need for donor corneas include pre-formed biosynthetic buttons and in situ-forming matrices that strive to achieve the transparency, biocompatibility, patient comfort, and biointegration that is possible with native tissue. Herein, we report on the development of an in situ-forming hydrogel of collagen type I crosslinked via multi-functional polyethylene glycol (PEG)-N-hydroxysuccinimide (NHS) and characterize its biophysical properties and regenerative capacity both in vitro and in vivo. The hydrogels form under ambient conditions within minutes upon mixing without the need for an external catalyst or trigger such as light or heat, and their transparency, degradability, and stiffness are modulated as a function of number of PEG arms and concentration of PEG. In addition, in situ-forming PEG-collagen hydrogels support the migration and proliferation of corneal epithelial and stromal cells on their surface. In vivo studies in which the hydrogels were formed in situ over stromal keratectomy wounds without sutures showed that they supported multi-layered surface epithelialization. Overall, the in situ forming PEG-collagen hydrogels exhibited physical and biological properties desirable for a corneal stromal defect wound repair matrix that could be applied without the need for sutures or an external trigger such as a catalyst or light energy.
K-PAM: a unified platform to distinguish Klebsiella species K- and O-antigen types, model antigen structures and identify hypervirulent strains
Authors: Patro L. Ponoop Prasad , Sudhakar Karpagam Uma , Rathinavelan Thenmalarchelvi 
Published: October 2020

AbstractA computational method has been developed to distinguish theKlebsiellaspecies serotypes to aid in outbreak surveillance. A reliability score (estimated based on the accuracy of a specific K-type prediction against the dataset of 141 distinct K-types) average (ARS) that reflects the specificity between theKlebsiellaspecies capsular polysaccharide biosynthesis and surface expression proteins, and their K-types has been established. ARS indicates the following order of potency in accurate serotyping: Wzx (ARS = 98.5%),Wzy (ARS = 97.5%),WbaP (ARS = 97.2%),Wzc (ARS = 96.4%),Wzb (ARS = 94.3%),WcaJ (ARS = 93.8%),Wza (ARS = 79.9%) and Wzi (ARS = 37.1%). Thus, Wzx, Wzy and WbaP can give more reliable K-typing compared with other proteins. A fragment-based approach has further increased the Wzi ARS from 37.1% to 80.8%. The efficacy of these 8 proteins in accurate K-typing has been confirmed by a rigorous testing and the method has been automated as K-PAM (www.iith.ac.in/K-PAM/). Testing also indicates that the use of multiple genes/proteins helps in reducing the K-type multiplicity, distinguishing the K-types that have identical K-locus (like KN3 and K35) and identifying the ancestral serotypes ofKlebsiellaspp. K-PAM has the facilities to O-type using Wzm (ARS = 85.7%) and Wzt (ARS = 85.7%) and identifies the hypervirulentKlebsiellaspecies by the use ofrmpA, rmpA2,iucA,iroBandpeg-344marker genes. Yet another highlight of the server is the repository of the modeled 11 O- and 79 K- antigen 3D structures.
Establishment of a fluorescence staining method for Schistosoma japonicum miracidia
Authors: Shen Yuanxi , Ji Rongyi , Yang Man , Lin Jiaojiao , Wang Hairong , Zhu Chuangang , Xu Qinkun 
Published: October 2020

AbstractCurrently the diagnosis of schistosomiasis is mainly determined by observing the presence of eggs in host stool samples. Because of the overwhelming number of impurities in the stool, eggs are rarely observed. Therefore, the stool hatching method is used to observe the miracidia in the water. However, the miracidia ofSchistosoma japonicumare small and difficult to detect, and missed detection is likely to occur when the infection level is low. In this study, recombinant streptococcal protein G-enhanced green fluorescent protein (rSPG-EGFP) was expressed, purified, and used as a fluorescence staining reagent for miracidia. A preliminary miracidium fluorescence staining method based on antigen and antibody bindingwas established by combining recombinant protein staining with the stool hatching method. The stool hatching method was used to collect the miracidia ofS. japonicum, andSchistosoma-positive serum and the recombinant protein were mixed to assess the feasibility of fluorescence staining of miracidia. The miracidia ofS. japonicumandSchistosoma turkestanicumwere incubated withS. japonicum-positive serum andS. turkestanicum-positive serum, respectively, to identify miracidia species. When the fluorescence staining method was used to observe living miracidia, the miracidiawere labelled by the recombinant protein, and their motility status was not affected.
Divalent heavy metals and uranyl cations incorporated in calcite change its dissolution process
Authors: Zhang Xiaohang , Guo Jianan , Wu Shijun , Chen Fanrong , Yang Yongqiang 
Published: October 2020

AbstractDue to the high capacity of impurities in its structure, calcite is regarded as one of the most attractive minerals to trap heavy metals (HMs) and radionuclides via substitution during coprecipitation/crystal growth. As a high-reactivity mineral, calcite may release HMs via dissolution. However, the influence of the incorporated HMs and radionuclides in calcite on its dissolution is unclear. Herein, we reported the dissolution behavior of the synthesized calcite incorporated with cadmium (Cd), cobalt (Co), nickel (Ni), zinc (Zn), and uranium (U). Our findings indicated that the HMs and U in calcite could significantly change the dissolution process of calcite. The results demonstrated that the incorporated HMs and U had both inhibiting and enhancing effects on the solubility of calcite, depending on the type of metals and their content. Furthermore, secondary minerals such as smithsonite (ZnCO3), Co-poor aragonite, and U-rich calcite precipitated during dissolution. Thus, the incorporation of metals into calcite can control the behavior of HMs/uranium, calcite, and even carbon dioxide.
Detection of tuberculosis in cynomolgus macaques (Macaca fascicularis) using a supplementary Monkey Interferon Gamma Releasing Assay (mIGRA)
Authors: Warit S. , Billamas P. , Makhao N. , Jaitrong S. , Juthayothin T. , Yindeeyoungyeon W. , Dokladda K. , Smittipat N. , Kemthong T. , Meesawat S. , Kongsombat N. , Kraitat C. , Prammananan T. , Palaga T. , Chaiprasert A. , Malaivijitnond S. 
Published: October 2020

AbstractCynomolgus monkeys (Macaca fascicularis; MF) are commonly used as nonhuman primate models for pharmaceutical product testing. In their habitat range, monkeys have close contact with humans, allowing the possibility of bidirectional transmission of tuberculosis (TB) between the two species. Although the intradermal tuberculin skin test (TST) is used for TB detection in MF, it has limitations. Herein, we established the mIGRA, combining human QuantiFERON-TB Gold-Plus and monkey IFN-γ ELISApro systems, and used it to investigate 39 captive MF who were cage-mates or lived in cages located near a monkey who died from the naturally TB infection. During a 12-month period of study, 14 (36%), 10 (26%), and 8 (21%) monkeys showed TB-positive results using the mIGRA, the TST, and TB culture, respectively. Among the 14 mIGRA-positive monkeys, 8 (57.1%) were TST-positive and 7 (50%) were culture-positive, indicating early TB detection in the latent and active TB stages with the mIGRA. Interestingly, 3 (37.5%) of the TST-negative monkeys were culture-positive. Our study showed that the mIGRA offers many advantages, including high sensitivity and high throughput, and it requires only one on-site visit to the animals. The assay may be used as a supplementary tool for TB screening in MF.
Synaptic transistors with aluminum oxide dielectrics enabling full audio frequency range signal processing
Authors: Bolat Sami , Torres Sevilla Galo , Mancinelli Alessio , Gilshtein Evgeniia , Sastre Jordi , Cabas Vidani Antonio , Bachmann Dominik , Shorubalko Ivan , Briand Danick , Tiwari Ayodhya N. , Romanyuk Yaroslav E. 
Published: October 2020

AbstractThe rapid evolution of the neuromorphic computing stimulates the search for novel brain-inspired electronic devices. Synaptic transistors are three-terminal devices that can mimic the chemical synapses while consuming low power, whereby an insulating dielectric layer physically separates output and input signals from each other. Appropriate choice of the dielectric is crucial in achieving a wide range of operation frequencies in these devices. Here we report synaptic transistors with printed aluminum oxide dielectrics, improving the operation frequency of solution-processed synaptic transistors by almost two orders of magnitude to 50 kHz. Fabricated devices, yielding synaptic response for all audio frequencies (20 Hz to 20 kHz), are employed in an acoustic response system to show the potential for future research in neuro-acoustic signal processing with printed oxide electronics.
Cerebello-thalamo-cortical network is intrinsically altered in essential tremor: evidence from a resting state functional MRI study
Authors: Nicoletti Valentina , Cecchi Paolo , Pesaresi Ilaria , Frosini Daniela , Cosottini Mirco , Ceravolo Roberto 
Published: October 2020

AbstractCerebello-thalamo-cortical network is suggested to be involved in the pathophysiology of Essential Tremor (ET). 23 patients with ET and 23 matched HC underwent a 3T-MRI with acquisition of a resting state sequence. Connectivity was investigated using a seed-based regression analyses approach. In ET patients were observed:

Reduced connectivity between left primary motor cortex (M1) seed and right premotor cortex and cerebellum and bilateral premotor, parietal areas, supplementary motor area (SMA);

Increased connectivity between left somatosensory cortex (S1) seed and parietal areas, M1, premotor cortex, SMA; reduced connectivity of this seed with cerebellum.

Increased connectivity of SMA seed with premotor cortex and decreased with parietal and precentral areas;

Increased connectivity between left thalamus seed and cerebellum;

Reduced connectivity between right cerebellum seeds and other cerebellar areas, precentral and premotor areas.

ET showed altered connectivity within the cortical sensory-motor network and between cerebral cortex and cerebellum. The increased connectivity between cerebellum and thalamus is consistent with their crucial role in tremor generation. These findings support the dynamical entrainment of multiple central oscillators throughout the cerebello-thalamo-cortical network in ET. This evidence is strengthened by the finding that this network is altered also when the core symptom is absent.
Unlocking the mystery of the mid-Cretaceous Mysteriomorphidae (Coleoptera: Elateroidea) and modalities in transiting from gymnosperms to angiosperms
Authors: Peris David , Kundrata Robin , Delclòs Xavier , Mähler Bastian , Ivie Michael A. , Rust Jes , Labandeira Conrad C. 
Published: October 2020

AbstractThe monospecific family Mysteriomorphidae was recently described based on two fossil specimens from the Late Cretaceous Kachin amber of northern Myanmar. The family was placed in Elateriformia incertae sedis without a clear list of characters that define it either in Elateroidea or in Byrrhoidea. We report here four additional adult specimens of the same lineage, one of which was described using a successful reconstruction from a CT-scan analysis to better observe some characters. The new specimens enabled us to considerably improve the diagnosis of Mysteriomorphidae. The family is definitively placed in Elateroidea, and we hypothesize its close relationship with Elateridae. Similarly, there are other fossil families of beetles that are exclusively described from Cretaceous ambers. These lineages may have been evolutionarily replaced by the ecological revolution launched by angiosperms that introduced new co-associations with taxa. These data indicate a macroevolutionary pattern of replacement that could be extended to other insect groups.
Analysis of factors affecting reversal of Hartmann’s procedure and post-reversal complications
Authors: Kang Jae Hyun , Kang Byung Mo , Yoon Sang Nam , Kim Jeong Yeon , Park Jun Ho , Oh Bo Young , Kim Jong Wan 
Published: October 2020

AbstractAlthough Hartmann’s procedure (HP) is commonly used as emergency treatment for colorectal disease, the reversal of HP (HR) is infrequently performed. The aims were to evaluate the rate of HR and determine the factors predictive of achieving HR. We retrospectively reviewed the medical records of patients who underwent HP between January 2007 and June 2019 at six Hallym University-affiliated hospitals. Multivariable analysis was performed to identify which factors were independently associated with HR. In the study period, 437 patients underwent HP, and 127 (29.0%) subsequently underwent HR. Of these, 46 (35.9%) patients experienced post-HR complications. In multivariable analysis, an interval between HP and HR of > 6 months was associated with the only lower rate of post-HR complications. Multivariate analysis showed that HR was less likely in patients aged > 70 years, those with American Society of Anesthesiologists (ASA) class III or IV, elective surgery, those experiencing more than two HP-related complications, and those with a malignancy (an indication for HP). One-third of the patients underwent HR. Age > 70 years, ASA class III or IV, elective surgery, more than two HP-related complications, and malignancy were associated with a non-HR rate.
Origin of the biphase nature and surface roughness of biogenic calcite secreted by the giant barnacle Austromegabalanus psittacus
Authors: Checa Antonio G. , Macías-Sánchez Elena , Rodríguez-Navarro Alejandro B. , Sánchez-Navas Antonio , Lagos Nelson A. 
Published: October 2020

AbstractThe calcite grains forming the wall plates of the giant barnacle Austramegabalanus psittacus have a distinctive surface roughness made of variously sized crystalline nanoprotrusions covered by extremely thin amorphous pellicles. This biphase (crystalline-amorphous) structure also penetrates through the crystal’s interiors, forming a web-like structure. Nanoprotrusions very frequently elongate following directions related to the crystallographic structure of calcite, in particular, the <− 441> directions, which are the strongest periodic bond chains (PBCs) in calcite. We propose that the formation of elongated nanoprotrusions happens during the crystallization of calcite from a precursor amorphous calcium carbonate (ACC). This is because biomolecules integrated within the ACC are expelled from such PBCs due to the force of crystallization, with the consequent formation of uninterrupted crystalline nanorods. Expelled biomolecules accumulate in adjacent regions, thereby stabilizing small pellicle-like volumes of ACC. With growth, such pellicles become occluded within the crystal. In summary, the surface roughness of the biomineral surface reflects the complex shape of the crystallization front, and the biphase structure provides evidence for crystallization from an amorphous precursor. The surface roughness is generally explained as resulting from the attachment of ACC particles to the crystal surface, which later crystallised in concordance with the crystal lattice. If this was the case, the nanoprotrusions do not reflect the size and shape of any precursor particle. Accordingly, the particle attachment model for biomineral formation should seek new evidence.
Kynurenic acid is a potential overlapped biomarker between diagnosis and treatment response for depression from metabolome analysis
Authors: Erabi Hisayuki , Okada Go , Shibasaki Chiyo , Setoyama Daiki , Kang Dongchon , Takamura Masahiro , Yoshino Atsuo , Fuchikami Manabu , Kurata Akiko , Kato Takahiro A. , Yamawaki Shigeto , Okamoto Yasumasa 
Published: October 2020

AbstractSince optimal treatment at an early stage leads to remission of symptoms and recovery of function, putative biomarkers leading to early diagnosis and prediction of therapeutic responses are desired. The current study aimed to use a metabolomic approach to extract metabolites involved in both the diagnosis of major depressive disorder (MDD) and the prediction of therapeutic response for escitalopram. We compared plasma metabolites of MDD patients (n = 88) with those in healthy participants (n = 88) and found significant differences in the concentrations of 20 metabolites. We measured the Hamilton Rating Scale for Depression (HRSD) on 62 patients who completed approximately six-week treatment with escitalopram before and after treatment and found that kynurenic acid and kynurenine were significantly and negatively associated with HRSD reduction. Only one metabolite, kynurenic acid, was detected among 73 metabolites for overlapped biomarkers. Kynurenic acid was lower in MDD, and lower levels showed a better therapeutic response to escitalopram. Kynurenic acid is a metabolite in the kynurenine pathway that has been widely accepted as being a major mechanism in MDD. Overlapping biomarkers that facilitate diagnosis and prediction of the treatment response may help to improve disease classification and reduce the exposure of patients to less effective treatments in MDD.
Adaptation to pitch-altered feedback is independent of one’s own voice pitch sensitivity
Authors: Alemi Razieh , Lehmann Alexandre , Deroche Mickael L. D. 
Published: October 2020

AbstractMonitoring voice pitch is a fine-tuned process in daily conversations as conveying accurately the linguistic and affective cues in a given utterance depends on the precise control of phonation and intonation. This monitoring is thought to depend on whether the error is treated as self-generated or externally-generated, resulting in either a correction or inflation of errors. The present study reports on two separate paradigms of adaptation to altered feedback to explore whether participants could behave in a more cohesive manner once the error is of comparable size perceptually. The vocal behavior of normal-hearing and fluent speakers was recorded in response to a personalized size of pitch shift versus a non-specific size, one semitone. The personalized size of shift was determined based on the just-noticeable difference in fundamental frequency (F0) of each participant’s voice. Here we show that both tasks successfully demonstrated opposing responses to a constant and predictable F0 perturbation (on from the production onset) but these effects barely carried over once the feedback was back to normal, depicting a pattern that bears some resemblance to compensatory responses. Experiencing a F0 shift that is perceived as self-generated (because it was precisely just-noticeable) is not enough to force speakers to behave more consistently and more homogeneously in an opposing manner. On the contrary, our results suggest that the type of the response as well as the magnitude of the response do not depend in any trivial way on the sensitivity of participants to their own voice pitch. Based on this finding, we speculate that error correction could possibly occur even with a bionic ear, typically even when F0 cues are too subtle for cochlear implant users to detect accurately.
Improved cis-Abienol production through increasing precursor supply in Escherichia coli
Authors: Cheng Tao , Zhao Guang , Xian Mo , Xie Congxia 
Published: October 2020

Abstractcis-Abienol, a natural diterpene-diol isolated from balsam fir (Abies balsamea), can be employed as precursors for the semi-synthesis of amber compounds, which are sustainable replacement for ambergris and widely used in the fragmented industry. This study combinatorially co-expressed geranyl diphosphate synthase, geranylgeranyl diphosphate synthase, Labda-13-en-8-ol diphosphate synthase and diterpene synthase, with the best combination achieving ~ 0.3 mg/L of cis-abienol. An additional enhancement of cis-abienol production (up to 8.6 mg/L) was achieved by introducing an exogenous mevalonate pathway which was divided into the upper pathway containing acetyl-CoA acetyltransferase/HMG-CoA reductase and HMG-CoA synthase and the lower pathway containing mevalonate kinase, phosphomevalonate kinase, pyrophosphate mevalonate decarboxylase and isopentenyl pyrophosphate isomerase. The genetically modified strain carrying chromosomal copy of low genes of the mevalonate with the trc promoter accumulated cis-abienol up to 9.2 mg/L in shake flask. Finally, cis-abienol titers of ~ 220 mg/L could be achieved directly from glucose using this de novo cis-abienol-producing E. coli in high-cell-density fermentation. This study demonstrates a microbial process to apply the E. coli cell factory in the biosynthesis of cis-abienol.
Impacts of climate change and human activities on the water discharge and sediment load of the Pearl River, southern China
Authors: Wei Xing , Cai Shuqun , Ni Peitong , Zhan Weikang 
Published: October 2020

AbstractGlobal climate change and human activities have important effects on the water discharge and sediment load of the Pearl River. In this study, the water discharge and sediment load were investigated by using hydro-meteorological data from 1954 to 2018. The linear regression, Mann–Kendall abrupt test and double mass curve were employed to detect trends and abrupt change-points in water discharge and sediment load and to quantify the effects of climate change and human activities on water discharge and sediment load. The results revealed that the annual sediment load exhibited a significant decreasing trend at a rate of − 2.24 × 104 t/year, regardless of water discharge, and an abrupt change occurred in 1998. Human activities, especially dam construction contributed 96% to this change, while 4% was due to climate change. El Niño/Southern Oscillation (ENSO) events are often associated with low precipitation, resulting in low water discharge and sediment load, indicating that changes in ENSO periodicity could affect the inter-annual periodic variations of water discharge and sediment load. As population and economy boom, more dams are being built in the Pearl River basin, and special attention should be paid to the management and mitigation of the effects of dams on sediment load.