bluuebunny/crossref_metadata_2025_split huggingface dataset:
Harmonization of color measurements for dental application
Authors: Paravina Rade D. , Pereira Sanchez Natalie A. , Tango Rubens N. 
Published: August 2020

AbstractThis multicenter study evaluated the effect of harmonizing spectrophotometric (d/8°) color measurements by using actual dental materials of clinically relevant thickness and translucency as “calibration tiles.” Ten tooth‐colored, 1‐mm thick, resin‐ and ceramic‐based materials were tested at the coordinating center (CC) and six research sites (RS). Correction factors were calculated for each material and each site to obtain the corrected reflection spectra from the non‐corrected data set. Non‐corrected (NC) and corrected (C) reflection values were converted into CIEDE2000 and CIELAB color parameters for the following comparisons: CC vs RSNC and CC vs RSC, between RSNC vs RSNC and RSC vs RSC pairs, and among RS separately (α = 0.05). Mean color differences for CC vs RSNC and corresponding CC vs RSC values were ΔE00 = 4.2 (2.6) and ΔE00 = 0.7 (0.6), respectively. Corresponding values for RSNC vs RSNC and RSC vs RSC specimen pairs were ΔE00 = 5.9 (2.9) and ΔE00 = 1.0 (0.5), respectively. The CIEDE2000 NC vs C comparison for each research site separately ranged from 0.5 to 8.5. Color differences of CC vs RSC and RSC vs RSC pairs decreased 83% to 84.5% as compared with respective color differences for non‐corrected research site measurements and was RS dependent (P < .001). The obtained results validated the proposed method of harmonizing color measurements for dental application as a reliable quality control tool for dental research laboratories and industry.
Design, synthesis and biological evaluation of several aromatic substituted chalcones as antimalarial agents
Authors: Gopinathan Adarsh , Moidu Mahreen , Mukundan Minil , Ellickal Narayanan Siju , Narayanan Hariraj , Adhikari Navin 
Published: August 2020

AbstractMalaria is a communicable disease which is caused by protozoan's mainly Plasmodium species (P. falciparum, P. ovale, P. vivax, P. malariae and P. knowlesi). The increasing resistance of Plasmodium to available malarial drugs poses a great responsibility for the researchers in the field of malaria. To overcome this problem of resistance, this study aimed to design and synthesize a new class of antimalarial agent with chalcone as the main moiety. Chalcones, a member of flavanoid family, consist of two aromatic rings of 1,3‐diphenyl‐2‐propen‐1‐one linked by a three carbon α,β‐unsaturated carbonyl system. Five derivatives were designed and among them one was selected. The CC2 was then synthesized by esterification of Para amino acetophenone followed by treatment with hydrazide to form 2‐(4 acetylphenoxy)acetohydrazide. This was then coupled with 2‐Bromo substituted Diazotized esterified anilines, which was finally linked with substituted benzaldehyde to yield CC2. These were then structurally verified by Infra Red (IR) and Nuclear Magnetic Resonance (NMR) spectroscopy. The chalcone was then tested for in vitro growth inhibition assays using SYBR GREEN‐1 Based assay and IC50 values were identified. The compound CC2 showed quite promising antimalarial activity by inhibiting cysteine protease enzyme. The acute toxicity studies of the compound were carried out as per OECD guideline 425 and the results showed no toxic signs and symptoms indicating CC2 as a safe and less toxic compound.
An overview of superficial radiation of surgical keloidectomy sites to reduce keloid recurrences
Authors: Berman Brian 
Published: August 2020

AbstractBackgroundKeloids adversely impact medical, cosmetic and psychological health and are detrimental to the quality of life of those affected. The clinical challenge following surgical excision of keloids is the high, 71%, recurrence rate. Based on its ability to suppress wound healing, judicious use of low‐dose, fractionated superficial radiation therapy (SRT) has been successful in reducing post‐keloidectomy keloid recurrences.ObjectiveThis overview focusses on the reported safety and efficacies of differing dosing and fractionation regimens of SRT of keloidectomy surgical sites to reduce keloid.MethodsA literature review regarding superficial radiation of surgical keloidectomy sites to reduce keloid recurrences.ResultsPostsurgical treatment of keloid excision suture lines with several fractions of SRT reduces keloid recurrence rates. An effective and safe treatment protocol is a biologically effective dose of 3000 cGy (BED 30) with three fractions of 600 cGy on postoperative days 1, 2, and 3. Fractionation of the SRT dose reduces the risk of hyperpigmentation and other adverse events.ConclusionJudicious use of low‐dose, fractionated superficial radiation therapy safely and effectively reduces the rate of post‐keloidectomy keloid recurrences.
ESC EORP Cardiomyopathy Registry: real‐life practice of genetic counselling and testing in adult cardiomyopathy patients
Authors: Heliö Tiina , Elliott Perry , Koskenvuo Juha W. , Gimeno Juan R. , Tavazzi Luigi , Tendera Michal , Kaski Juan Pablo , Mansencal Nicolas , Bilińska Zofia , Carr‐White Gerry , Damy Thibaud , Frustaci Andrea , Kindermann Ingrid , Ripoll‐Vera Tomas , Čelutkienė Jelena , Axelsson Anna , Lorenzini Massimiliano , Saad Aly , Maggioni Aldo P. , Laroche Cécile , Caforio Alida L.P. , Charron Philippe ,   EORP Cardiomyopathy Registry Investigators Group
Published: August 2020

AbstractAimsCardiomyopathies comprise a heterogeneous group of diseases, often of genetic origin. We assessed the current practice of genetic counselling and testing in the prospective European Society of Cardiology EURObservational Research Programme Cardiomyopathy Registry.Methods and resultsA total of 3208 adult patients from 69 centres in 18 countries were enrolled. Genetic counselling was performed in 60.8% of all patients [75.4% in hypertrophic cardiomyopathy (HCM), 39.2% in dilated cardiomyopathy (DCM), 70.8% in arrhythmogenic right ventricular cardiomyopathy (ARVC), and 49.2% in restrictive cardiomyopathy (RCM), P < 0.001]. Comparing European geographical areas, genetic counselling was performed from 42.4% to 83.3% (P < 0.001). It was provided by a cardiologist (85.3%), geneticist (15.1%), genetic counsellor (11.3%), or a nurse (7.5%) (P < 0.001). Genetic testing was performed in 37.3% of all patients (48.8% in HCM, 18.6% in DCM, 55.6% in ARVC, and 43.6% in RCM, P < 0.001). Index patients with genetic testing were younger at diagnosis and had more familial disease, family history of sudden cardiac death, or implanted cardioverter defibrillators but less co‐morbidities than those not tested (P < 0.001 for each comparison). At least one disease‐causing variant was found in 41.7% of index patients with genetic testing (43.3% in HCM, 33.3% in DCM, 51.4% in ARVC, and 42.9% in RCM, P = 0.13).ConclusionsThis is the first detailed report on the real‐life practice of genetic counselling and testing in cardiomyopathies in Europe. Genetic counselling and testing were performed in a substantial proportion of patients but less often than recommended by European guidelines and much less in DCM than in HCM and ARVC, despite evidence for genetic background.
Synthesis, Characterization and Investigation of Electrochemical and Spectroelectrochemical Properties of Peripherally Tetra Diethoxypropan Substituted Phthalocyanines
Authors: Akyüz Duygu , Akçay Hakkı Türker , Bayrak Rıza 
Published: August 2020

AbstractIn this study novel peripherally tetra 1,3‐diethoxy‐2‐propanol substituted Co(II), Cu(II), Mg(II), Ni(II) Zn(II) phthalocyanine derivatives were prepared. The characterization of the novel compounds was made by FT‐IR, 1H‐NMR, UV‐vis and MALDI‐TOF mass. Electrochemical, in‐situ spectroelectrochemical and electrocolorimetric measurements have been carried out with the aim to determine the redox properties, to illuminate reaction mechanism and the effect of substituent and metal center. Moreover, the color properties and aggregation tendency of compounds were also investigated.
Hepatic Fat—Genetic Risk Score Predicts Hepatocellular Carcinoma in Patients With Cirrhotic HCV Treated With DAAs
Authors: Degasperi Elisabetta , Galmozzi Enrico , Pelusi Serena , D’Ambrosio Roberta , Soffredini Roberta , Borghi Marta , Perbellini Riccardo , Facchetti Floriana , Iavarone Massimo , Sangiovanni Angelo , Valenti Luca , Lampertico Pietro 
Published: August 2020

Background and Aims
Genetic factors and steatosis predispose to hepatocellular carcinoma (HCC) in patients with chronic hepatitis C virus; however, their impact in patients with cirrhosis cured by direct‐acting antivirals (DAAs) is still undefined. We assessed the association between a genetic risk score (GRS) of hepatic fat accumulation, combining variants in PNPLA3 (patatin‐like phospholipase domain containing 3), MBOAT7 (membrane bound O‐acyltransferase domain containing 7), TM6SF2 (transmembrane 6 superfamily member 2), GCKR (glucokinase regulator), and HCC in patients treated with DAAs.

Approach and Results
We considered 509 consecutive patients with HCV cirrhosis (defined histologically or when liver stiffness ≥12 kPa) treated with DAAs. HCC was diagnosed according to international recommendations. GRS was calculated from the weighted impact of single variants on hepatic fat content quantified by H1 spectrometry in the general population (Dallas Heart Study). During a median follow‐up of 43 (3‐57) months after DAA start, 36 of 452 (8%) patients developed de novo HCC, 4‐year cumulative probability being 9% (95% confidence interval 7%‐12%). Male sex (hazard ratio [HR] 2.54, P = 0.02), diabetes (HR 2.39, P = 0.01), albumin (HR 0.35, P = 0.001), and GRS score >0.597 (HR 2.30, P = 0.04) were independent predictors of de novo HCC. In contrast, single genetic risk variants were not useful in stratifying HCC risk. The proportion of patients who developed HCC according to the combination of the independent risk factors ranged from 11% to 67%. HCC recurred in 28 of 57 (49%) patients with previous history; diabetes and ethnicity were the only independent predictors of HCC recurrence.

Conclusions
In a large cohort of DAA‐treated patients with cirrhotic HCV, GRS was associated with de novo HCC independently of classical risk factors, including liver disease severity. These data suggest that hepatic fat (i.e., lipotoxicity) promotes HCC in this setting and may represent a target for chemoprevention. Combination of clinical and genetic predictors may improve HCC risk stratification.
Online feedback and crowdfunding finance in China
Authors: Shahab Yasir , Riaz Yasir , Ntim Collins G. , Ye Zhiwei , Zhang Qingjing , Feng Ran 
Published: August 2020

AbstractThis study examines the impact of online feedback on the extent of alternative startups' fundraising success or failure through reward‐based online crowdfunding platforms. By drawing on regulatory focus theory, we theorize that online feedback relating to products or services is crucial in determining the success of entrepreneurial projects. While employing a unique dataset of 620 projects from renowned reward‐based Chinese crowdfunding platform “Demohour” (a major pioneer Chinese crowdfunding platform), the findings show a significant effect of online feedback on the extent of success or failure of reward‐based crowdfunding campaigns. Our findings, which are robust to different measures and methodologies imply that nascent entrepreneurs need to pay keen attention to online feedback about their innovative projects if they are to be successful in their fundraising efforts.
Effects of methylparaben on in vitro maturation of porcine oocytes
Authors: Barajas‐Salinas Adyeni , Ducolomb Yvonne , Betancourt Miguel , Núñez‐Macías Elivier , López Alma , Barraza Jaime , Quezadas‐Fuentes Juan , Bahena‐Ocampo Iván , Bonilla Edmundo , Retana‐Márquez Socorro , Casas Eduardo , Casillas Fahiel 
Published: August 2020

AbstractParabens (PBs) are compounds widely used in industry for food and personal care products as antimicrobials and preservatives. Their indiscriminate use has resulted in their detection in different ecosystems so that humans and other organisms are highly exposed. Methylparaben (MePB), compared with other PBs, is mostly detected in food, personal care and baby care products. PBs could be linked to the generation of hormonal disorders and fertility impairment since their recent classification as endocrine disruptors. The knowledge of the effects that MePB can exert is of great importance as, in terms of reproduction, information is limited. Therefore, the objective of the present study was to evaluate the effect of MePB on porcine oocyte viability and in vitro maturation (IVM), as well as to determine the lethal concentration at 50% (LC50) and the maturation inhibition concentration at 50% (MIC50). Oocytes were exposed to different MePB concentrations 0 (control), 50, 100, 500, 750 and 1000 μm during 44 h of IVM. Cytoplasmic alterations and reduced cumulus cell expansion were observed in oocytes exposed to MePB; however, viability was not affected. In addition, oocyte maturation decreased in a concentration‐dependent manner after exposure to MePB. The estimated LC50 was 2028.38 μm, whereas MIC50 was 780.31 μm. To our knowledge, this is the first study that demonstrates that MePB altered porcine oocyte morphology, and caused a reduction in cumulus cell expansion, both of which resulted in decreased oocyte maturation. Therefore, MePB exposure may be one of the factors involved in fertility impairment in mammals, including that of humans.
Thermal variability during ectotherm egg incubation: A synthesis and framework
Authors: Massey Melanie D. , Hutchings Jeffrey A. 
Published: August 2020

AbstractNatural populations of ectothermic oviparous vertebrates typically experience thermal variability in their incubation environment. Yet an overwhelming number of laboratory studies incubate animals under constant thermal conditions that cannot capture natural thermal variability. Here, we systematically searched for studies that incubated eggs of ectothermic vertebrates, including both fishes and herpetofauna, under thermally variable regimes. We ultimately developed a compendium of 66 studies that used thermally variable conditions for egg incubation. In this review, we qualitatively discuss key findings from literature in the compendium, including the phenotypic effects resulting from different patterns of thermally variable incubation, as well as the ontogenetic persistence of these effects. We also describe a physiological framework for contextualizing some of these effects, based on thermal performance theory. Lastly, we highlight key gaps in our understanding of thermally variable incubation and offer suggestions for future studies.
Thermal physiology responds to interannual temperature shifts in a montane horned lizard, Phrynosoma orbiculare
Authors: Domínguez‐Guerrero Saúl F. , Bodensteiner Brooke L. , Pardo‐Ramírez Alexis , Aguillón‐Gutierrez David R. , Méndez‐de la Cruz Fausto R. , Muñoz Martha M. 
Published: August 2020

AbstractAs climate change marches on, rapidly rising temperatures shatter records every year, presenting ever‐growing physiological challenges to organisms worldwide. Ectotherms rely on behavioral and physiological plasticity to contend with environmental fluctuations. Nonetheless, our understanding of thermal plasticity has been largely limited to laboratory settings. Here, we test whether aspects of thermal physiology respond to interannual shifts in thermal environment in a natural population of Phrynosoma orbiculare, a montane horned lizard, from Hidalgo, Mexico. At our field site, 2019 was markedly warmer than the year that preceded it. We detected population‐level increases in three key thermal physiological traits: preferred temperature, the critical thermal minimum, and the critical thermal maximum. Thus, thermal phenotypes appear to shift in tandem in response to environmental fluctuations. A subset of individuals were resampled across years, allowing insight into plastic shifts within an organism's lifetime. We detected parallel increases in these lizards for the preferred temperature and the critical thermal minimum, but not for the critical thermal maximum. Our results support a growing body of literature indicating that preferred conditions and cold tolerance can be highly labile over the course of an organism's lifetime, whereas hardening over shorter time periods is more common for heat tolerance. Given that heat tolerance increased at the population‐level, but not in resampled individuals, it is possible that rapid evolution occurred due to temperature increases. In short, physiological shifts can be observed in natural populations over relatively short timespans, and these shifts might reflect a combination of evolutionary and acclimatory responses.
Autologous stem cell rescue recipient with neutrophil tissue delivery detected prior to blood engraftment: A case report
Authors: Tbakhi Bushra , Furqan Fateeha , Scott Glynis , Liesveld Jane L. , Aljitawi Omar S. 
Published: August 2020

AbstractNeutrophil recovery after autologous hematopoietic cell transplantation (ASCT) is affirmed with achievement of an absolute neutrophil count (ANC) of ≥500/µL. There is growing evidence that neutrophils may be observed despite undetectable peripheral ANC counts following autologous hematopoietic cell transplant and are preferentially delivered to sites of inflammation. We report an interesting case that confirms neutrophil tissue delivery to the skin two days prior to evidence of blood engraftment after an ASCT.
Rumen bacteria and meat fatty acid composition of Sunit sheep reared under different feeding regimens in China
Authors: Wang Bohui , Luo Yulong , Wang Yu , Wang Debao , Hou Yanru , Yao Duo , Tian Jianjun , Jin Ye 
Published: August 2020

AbstractBACKGROUNDRumen bacteria play a critical role in feed degradation and productivity. This study evaluated the impact of feeding regimen on the rumen microbial populations and fatty acid composition of the meat of sheep. Twenty‐four Sunit sheep were raised on a grass pasture from birth to 9 months of age, at which time they were randomly divided into two feeding groups: pasture feeding (PF) and barn feeding (BF). Sheep in the PF group were allowed to graze freely on wild grassland for 3 months. Sheep in the BF group were confined for 3 months to a dry barn, in which they roamed freely with corn straw and corn.RESULTSSheep in the PF group had greater rumen bacteria diversity. The relative abundances of the genera Butyrivibrio_2, Saccharofermentans and Succiniclasticum were increased, and that of the genus RC9_gut_group was decreased, in the PF compared to the BF sheep. The n‐3 polyunsaturated fatty acid contents were greater in meat from PF sheep than from BF sheep. In addition, the α‐linolenic acid (C18:3 n‐3, ALA) and conjugated linoleic acid (CLA) contents were positively correlated with the abundance of Butyrivibrio_2.CONCLUSIONGrazing may improve the diversity of rumen bacteria and increase the proportion of ALA and CLA in sheep meat. © 2020 Society of Chemical Industry
Electrospinning and 3D bioprinting for intervertebral disc tissue engineering
Authors: De Pieri Andrea , Byerley Ann M. , Musumeci Catherine R. , Salemizadehparizi Fatemeh , Vanderhorst Maya A. , Wuertz‐Kozak Karin 
Published: August 2020

AbstractIntervertebral disc (IVD) degeneration is a major cause of low back pain and represents a massive socioeconomic burden. Current conservative and surgical treatments fail to restore native tissue architecture and functionality. Tissue engineering strategies, especially those based on 3D bioprinting and electrospinning, have emerged as possible alternatives by producing cell‐seeded scaffolds that replicate the structure of the IVD extracellular matrix. In this review, we provide an overview of recent advancements and limitations of 3D bioprinting and electrospinning for the treatment of IVD degeneration, focusing on future areas of research that may contribute to their clinical translation.
Leaching kinetics of electric arc furnace dust in nitric acid solutions
Authors: Zoraga Mert , Ilhan Sedat , Kalpakli Ahmet Orkun 
Published: August 2020

AbstractElectric arc furnace dust contains mainly ZnO, ZnFe2O4, and iron oxides. In this study, chemical composition of ZnO, ZnFe2O4, and Fe2O3 and leaching kinetics of ZnO, ZnFe2O4, and Fe2O3 in HNO3 solutions were investigated. It was seen that the dissolution of ZnO is very fast, therefore the leaching kinetics of ZnO cannot be determined. Kinetic parameters and model equations were derived for the leaching of ZnFe2O4 and Fe2O3. Leaching kinetics of ZnFe2O4 was explained by the pseudohomogeneous reaction model. Activation energy and order of HNO3 concentration were found to be as 37.5 kJ mol−1 and 0.37, respectively. The model equation was derived as . It was determined that experimental data for the leaching kinetics of Fe2O3 best fit with the shrinking core model (SCM). Activation energy and order of HNO3 concentration were found to be as 51.5 kJ mol−1 and 0.67, respectively The model equation was derived using SCM as .
RETRACTED: ASPM predicts poor prognosis and regulates cell proliferation in bladder cancer
Authors: Gao Zhen‐Ya , Yu Fang , Jia Huan‐Xia , Ye Zhuo , Yao Shi‐Jie 
Published: August 2020

AbstractBladder cancer (BCa) is one of the most common malignancies with high morbidity and mortality worldwide. In recent years, it is of great importance to investigate the molecular etiology associated with of BCa. Abnormal spindle‐like microcephaly associated gene (ASPM) is the human orthologous of the Drosophila abnormal spindle (asp) and the most commonly mutated gene of autosomal recessive primary microcephaly. ASPM is overexpressed in several types of cancer cell lines and affects the progression and development of multiple types of cancers. However, its possible role in BCa progression is still unclear. Herein, we demonstrated the possible involvement of ASPM in the progression of BCa. We noticed that high expression of ASPM was positively associated with the poor prognosis. Its knockdown could significantly inhibit the proliferation of BCa cells in vitro and in mice. Therefore, we thought ASPM could act as a promising therapeutic target for BCa.
Gene mutations associated with early onset familial Alzheimer’s disease in China: An overview and current status
Authors: Qin Qi , Yin Yunsi , Wang Yan , Lu Yuanyuan , Tang Yi , Jia Jianping 
Published: August 2020

AbstractBackgroundMutations of three causative genes, namely presenilin 1 (PSEN1), presenilin 2 (PSEN2), and amyloid precursor protein (APP), have been identified as the major causes of early‐onset familial Alzheimer's disease (EOFAD). The prevalence of causative gene mutations in patients with EOFAD has been reported in previous studies worldwide but remains unclear in China. The patients with these known mutations always show considerable clinical phenotypic variability. However, to date, there have been no detailed descriptions of the clinical phenotypes associated with these Chinese EOFAD mutations. Thus, the aim of this study was to describe all of the known mutations in three EOFAD causative genes and genotype–phenotype correlations in Chinese patients with EOFAD.MethodWe systematically searched the PubMed, MEDLINE, CNKI, VIP, and WAN‐FANG databases to find Chinese EOFAD mutations in reports from inception through May 2020.ResultWe identified 31 studies reporting mutations of three causative genes in China. 10 mutations in APP gene, 27 mutations in PSEN1 gene and six mutations in PSEN2 were discovered in Chinese EOFAD. This review summarized all these probably pathogenic mutations as well as its clinical features. To the best of our knowledge, this is the first systemic review of causative gene mutations in patients with EOFAD in China.ConclusionThe analysis of the genetic and clinical phenotype correlations in this review supports the idea that the clinical phenotype might be influenced by specific genetic defects. It also suggests genetic testing and genotype–phenotype correlations are important for the accurate diagnosis and for understanding disease‐associated pathways and might also improve disease therapy and prevention.