bluuebunny/crossref_metadata_2025_split huggingface dataset:
Estudo cienciométrico da dinâmica comportamental das publicações referentes ao tratamento da COVID-19 no primeiro semestre de 2020
Authors: Bellotto Ghabriel Luiz , Anschau Bruna Spironello , Moereira Camilla Scott , Silva Juliane Toffoli , Teixeira Kádima Nayara 
Published: December 2020

A primeira infecção causada pelo novo coronavírus ocorreu na China, em dezembro de 2019. Desde então, a busca por tratamentos para a COVID-19 tornou-se alvo de pesquisas internacionais. Apesar dos estudos terem iniciado logo após o surto viral na China, era esperado o aumento das publicações desses estudos pelo mundo todo após a declaração da Organização Mundial de Saúde sobre o estado pandêmico da doença. Dessa forma, este estudo teve como objetivo analisar a dinâmica das publicações científicas sobre o tratamento da COVID-19 no primeiro semestre de 2020. Para a coleta de dados utilizou-se a plataforma MEDLINE por meio da PubMed; os descritores adotados foram: “COVID-19 and Treatment”
e “SARS-CoV-2 and Treatment”, restringindo o período de publicação de 01/01/2020 a 30/06/2020. Dados mostraram que em janeiro de 2020, fase inicial da pandemia, o número de
publicações sobre o tema foi ínfimo em relação a junho, com a pandemia totalmente disseminada. O maior crescimento no número de publicações ocorreu entre fevereiro e março (8,3 vezes maior). No que se refere ao número de países envolvidos em publicações, observa-se que em janeiro, três países estavam envolvidos; enquanto, em maio havia 41 países. No período analisado, a China liderou com 152 publicações, seguida pelos Estados Unidos (141). Isso pode ser resultado do surgimento dos primeiros casos da doença e do caráter socioeconômico de ambos, que apesar dos distintos regimes políticos, têm a pesquisa científica como ponto importante para a continuidade do desenvolvimento.
Efeito imediato da técnica finger kazoo em pacientes com afecções laríngeas: estudo de casos
Authors: Rossa Aline Medianeira Tolfo , Bastilha Gabriele Rodrigues , Christmann Mara Keli , Cielo Carla Aparecida 
Published: December 2020

Objetivo: Verificar as modificações vocais perceptivoauditivas após a técnica vocal finger kazoo em três mulheres com afecções laríngeas. Métodos: Três mulheres adultas com respectivo diagnóstico médico de: sulco vocal estria maior (S1), nódulos vocais (S2), e microweb laríngeo (S3) realizaram três séries de 15 repetições do finger kazoo com 30 s de repouso passivo entre as séries. Dois juízes realizaram análise vocal perceptivoauditiva antes, imediatamente após a terapia, e cinco minutos após silêncio absoluto. Resultados: Soprosidade, astenia e instabilidade diminuíram imediatamente após a execução do finger kazoo em S1 e S3 e soprosidade e instabilidade diminuíram em S2. Após cinco minutos de silêncio, todos os parâmetros diminuíram, com exceção da tensão em S1; a rouquidão, soprosidade e instabilidade diminuíram em S2; e todos os parâmetros, com exceção da tensão, pioraram em S3. Conclusões: O finger kazoo executado em uma sessão de três séries de 15 repetições ocasionou melhora de parâmetros perceptivoauditivos nas três mulheres com afecção laríngea em ambos os momentos pós-terapia, com exceção da paciente com microweb que apresentou melhora imediatamente após a realização da técnica e piora após cinco minutos de silêncio.
On some properties of the lattice of totally σ-local formations of finite groups
Authors: Safonov Vasilly G. , Safonova Inna Nikolaevna 
Published: December 2020

Throughout this paper, all groups are finite. Let $σ=\{σ_i{}|i\in I\}$ be some partition of the set of all primes $\Bbb{P}$. If $n$ is an integer, $G$ is a group, and $\mathfrak{F}$ is a class of groups, then $σ(n)=\{σ_i{}|σ_i{}\cap \pi(n)\ne \emptyset\}$, $σ(G)=σ(|G|)$, and $σ(\mathfrak{F})=\cup _G{}_\in{}_\mathfrak{F}σ(G)$. A function $f$ of the form  $f\colon σ\to$ {formations of groups} is called a formation σ-function. For any formation $σ$-function $f$ the class $LF_σ(f)$ is defined as follows:
$LF_{\sigma}(f)=(G$ is a group $|G=1$ или $G\ne1$ and $G/O_σ{}'_i{}_,{}_σ{}_i{}(G)\in f(σ_i{})$ for all $σ_i{}\in σ(G))$.
If for some formation $σ$-function $f$ we have $\mathfrak{F}=LF_{\sigma}(f)$, then the class $\mathfrak{F}$ is called $σ$-local definition of $\mathfrak{F}$. Every formation is called 0-multiply $σ$-local. For $n$ > 0, a formation $\mathfrak{F}$ is called $n$-multiply $σ$-local provided either $\mathfrak{F}=(1)$ is the class of all identity groups or $\mathfrak{F}=LF_{\sigma}(f)$, where $f(σ_i{})$ is $(n – 1)$-multiply $σ$-local for all $σ_i{}\in σ(\mathfrak{F})$. A formation is called totally $σ$-local if it is $n$-multiply $σ$-local for all non-negative integer $n$. The aim of this paper is to study properties of the lattice of totally $σ$-local formations. In particular, we prove that the lattice of all totally $σ$-local formations is algebraic and distributive.
Profiling of H3K27Ac Reveals the Influence of Asthma on the Epigenome of the Airway Epithelium
Authors: McErlean Peter , Kelly Audrey , Dhariwal Jaideep , Kirtland Max , Watson Julie , Ranz Ismael , Smith Janet , Saxena Alka , Cousins David J. , Van Oosterhout Antoon , Solari Roberto , Edwards Michael R. , Johnston Sebastian L. , Lavender Paul 
Published: December 2020

BackgroundAsthma is a chronic airway disease driven by complex genetic–environmental interactions. The role of epigenetic modifications in bronchial epithelial cells (BECs) in asthma is poorly understood.MethodsWe piloted genome-wide profiling of the enhancer-associated histone modification H3K27ac in BECs from people with asthma (n = 4) and healthy controls (n = 3).ResultsWe identified n = 4,321 (FDR &lt; 0.05) regions exhibiting differential H3K27ac enrichment between asthma and health, clustering at genes associated predominately with epithelial processes (EMT). We identified initial evidence of asthma-associated Super-Enhancers encompassing genes encoding transcription factors (TP63) and enzymes regulating lipid metabolism (PTGS1). We integrated published datasets to identify epithelium-specific transcription factors associated with H3K27ac in asthma (TP73) and identify initial relationships between asthma-associated changes in H3K27ac and transcriptional profiles. Finally, we investigated the potential of CRISPR-based approaches to functionally evaluate H3K27ac-asthma landscape in vitro by identifying guide-RNAs capable of targeting acetylation to asthma DERs and inducing gene expression (TLR3).ConclusionOur small pilot study validates genome-wide approaches for deciphering epigenetic mechanisms underlying asthma pathogenesis in the airways.
Teasaponin Ameliorates Murine Colitis by Regulating Gut Microbiota and Suppressing the Immune System Response
Authors: Yang Huan , Cai Rui , Kong Ziyan , Chen Ying , Cheng Chen , Qi Suhua , Gu Bing 
Published: December 2020

Background: Dietary intervention is an exciting topic in current research of inflammatory bowel disease (IBD). The effect of teasaponin (TS) on IBD has not been fully elucidated. Here, we aim to investigate the intestinal anti-inflammatory activity of TS in a dextran sodium sulfate (DSS)-induced colitis mouse model and identify potential mechanisms.Methods: We applied TS to mice with DSS-induced colitis and then monitored the body weight, disease activity index (DAI) daily. When sacrificed, the intestinal permeability was measured. The analysis of mucin and tight junction proteins was conducted. We detected the inflammatory cytokines, the immune cells and related inflammatory signaling pathways. In addition, the gut microbiota were analyzed by 16S rRNA sequencing and we also performed fecal microbiota transplantation (FMT).Results: It showed that TS ameliorated the colonic damage by lowering the DAI, prolonging the colon length, reducing inflammatory cytokines and improving the mucus barrier. Parallel to down-regulation of the inflammatory cytokines, the fecal lipocalin 2, p-P65, p-STAT3, and neutrophil accumulation were also decreased in TS-treated mice. Microbiota characterization showed that Campylobacteria, Proteobacteria, Helicobacter, and Enterobacteriaceae were the key bacteria associated with IBD. In addition, TS could reverse the Firmicutes/Bacteroidetes (F/B) ratio and increase the beneficial bacteria, including Akkermansia and Bacteroides. TS ameliorated DSS-induced colitis by regulating the gut microbiota, and the gut microbiota could regulate gut inflammation.Conclusions: These studies demonstrated that TS ameliorated murine colitis through the modulation of immune response, mucus barrier and gut microbiota, thus improving gut dysbiosis. In addition, the gut microbiota may play an important role in regulating the host's innate immune system, and the two coexist and are mutually beneficial. We provide a promising perspective on the clinical treatment of IBD.
Side of Lesions Predicts Surgical Outcomes in Patients With Drug-Resistant Temporal Lobe Epilepsy Secondary to Focal Cortical Dysplasia Type IIIa
Authors: He Xinghui , Liu Dingyang , Yang Zhuanyi , Zhang Junmei , Li Sushan , Yang Zhiquan 
Published: December 2020

Objective: This study aims to evaluate the surgical outcomes and analyze the predictors of surgical outcomes in patients undergoing anterior temporal lobectomy (ATL) for drug-resistant temporal lobe epilepsy (TLE) secondary to focal cortical dysplasia (FCD) type IIIa.Methods: Data on patients with drug-resistant TLE secondary to FCD type IIIa who had undergone ATL at Xiangya Hospital, Central South University from January 2014 to April 2018, were collected retrospectively. International League Against Epilepsy (ILAE) classification was used to evaluate postoperative seizure outcomes. Predictors of surgical outcomes were identified by using univariate and multivariate analyses.Results: A total of 43 patients with drug-resistant TLE secondary to FCD type IIIa who had undergone ATL were included in this study. Twenty patients had right ATL, and 23 patients had left ATL. With a follow-up of 2–6 years, 76.7% (33 of 43) of patients were seizure-free. Univariate and multivariate analysis results indicated that lesions on the right side independently predict postoperative seizure freedom (OR, 0.08; 95% CI, 0.01–0.72; P = 0.024).Conclusion: ATL is an effective therapy for patients with drug-resistant TLE secondary to FCD type IIIa. Patients with lesions on the right side are more likely to achieve postoperative seizure freedom.
Exploratory Analysis of Qualitative MR Imaging Features for the Differentiation of Glioblastoma and Brain Metastases
Authors: Meier Raphael , Pahud de Mortanges Aurélie , Wiest Roland , Knecht Urspeter 
Published: December 2020

ObjectivesTo identify qualitative VASARI (Visually AcceSIble Rembrandt Images) Magnetic Resonance (MR) Imaging features for differentiation of glioblastoma (GBM) and brain metastasis (BM) of different primary tumors.Materials and MethodsT1-weighted pre- and post-contrast, T2-weighted, and T2-weighted, fluid attenuated inversion recovery (FLAIR) MR images of a total of 239 lesions from 109 patients with either GBM or BM (breast cancer, non-small cell (NSCLC) adenocarcinoma, NSCLC squamous cell carcinoma, small-cell lung cancer (SCLC)) were included. A set of adapted, qualitative VASARI MR features describing tumor appearance and location was scored (binary; 1 = presence of feature, 0 = absence of feature). Exploratory data analysis was performed on binary scores using a combination of descriptive statistics (proportions with 95% binomial confidence intervals), unsupervised methods and supervised methods including multivariate feature ranking using either repeated fitting or recursive feature elimination with Support Vector Machines (SVMs).ResultsGBMs were found to involve all lobes of the cerebrum with a fronto-occipital gradient, often affected the corpus callosum (32.4%, 95% CI 19.1–49.2), and showed a strong preference for the right hemisphere (79.4%, 95% CI 63.2–89.7). BMs occurred most frequently in the frontal lobe (35.1%, 95% CI 28.9–41.9) and cerebellum (28.3%, 95% CI 22.6–34.8). The appearance of GBMs was characterized by preference for well-defined non-enhancing tumor margin (100%, 89.8–100), ependymal extension (52.9%, 36.7–68.5) and substantially less enhancing foci than BMs (44.1%, 28.9–60.6 vs. 75.1%, 68.8–80.5). Unsupervised and supervised analyses showed that GBMs are distinctively different from BMs and that this difference is driven by definition of non-enhancing tumor margin, ependymal extension and features describing laterality. Differentiation of histological subtypes of BMs was driven by the presence of well-defined enhancing and non-enhancing tumor margins and localization in the vision center. SVM models with optimal hyperparameters led to weighted F1-score of 0.865 for differentiation of GBMs from BMs and weighted F1-score of 0.326 for differentiation of BM subtypes.ConclusionVASARI MR imaging features related to definition of non-enhancing margin, ependymal extension, and tumor localization may serve as potential imaging biomarkers to differentiate GBMs from BMs.
Antidepressant Use and the Risk of Major Adverse Cardiovascular Events in Patients Without Known Cardiovascular Disease: A Retrospective Cohort Study
Authors: Jang Ha Young , Kim Jae Hyun , Song Yun-Kyoung , Shin Ju-Young , Lee Hae-Young , Ahn Yong Min , Oh Jung Mi , Kim In-Wha 
Published: December 2020

Aims: Conflicting data exist on whether an association exists between antidepressants and the risk of major adverse cardiovascular events (MACEs) in patients with depression. This may be due to the use of various study designs and residual or unmeasured confounding. We aimed to assess the association between antidepressant use and the risk of MACEs while considering various covariates, including severity of depression and the cardiovascular disease (CVD) risk score.Methods: Patients newly diagnosed with depression with no history of ischemic heart disease and stroke were followed-up from 2009 to 2015. We conducted Cox proportional hazard regression analysis to estimate hazard ratios (HRs) for each antidepressant for MACE risk.Result: We followed-up (median, 4.4 years) 31,830 matched patients with depression (15,915 antidepressant users and 15,915 non-users). In most patients (98.7%), low-dose tricyclic antidepressants (TCAs) were related with a significantly increased risk of MACEs [adjusted HR = 1.20, 95% confidence interval (CI) = 1.03–1.40]. Duration response relationship showed a gradually increasing HR from 1.15 (95% CI = 0.98–1.33; &lt;30 days of use) to 1.84 (95% CI = 1.35–2.51; ≥365 days of use) (p for trend &lt;0.01). High Korean atherosclerotic CVD risk score (≥7.5%) or unfavorable lifestyle factors (smoking, alcohol intake, and exercise) were significantly associated with MACEs.Conclusion: Even at low doses, TCA use was associated with MACEs during primary prevention. Longer duration of TCA use correlated with higher HR. Careful monitoring is needed with TCA use in patients with no known CVD history.
Evaluation of Serum/Urine Genomic and Metabolomic Profiles to Improve the Adherence to Sildenafil Therapy in Patients with Erectile Dysfunction
Authors: Rocca Maria Santa , Vignoli Alessia , Tenori Leonardo , Ghezzi Marco , De Rocco Ponce Maurizio , Vatsellas Giannis , Thanos Dimitris , Padrini Roberto , Foresta Carlo , De Toni Luca 
Published: December 2020

Type V-phosphodiesterase-inhibitors (PDE5i) are the first choice drugs in the treatment of erectile dysfunction (ED), being effective in 60–70% of patients. However, approximately 50% of patients per year discontinue the treatment with PDE5i after reporting poor drug efficacy or major adverse drug reactions (ADR). To identify early markers of efficacy/safety for the treatment of ED with PDE5i, the basal clinical characteristics of patients, integrated with metabolomics analysis of serum and urine and genomic data, were here correlated with the PDE5i efficacy and the occurrence of ADR upon administration. Thirty-six males with new diagnosis of ED were consecutively recruited and characterized at baseline for anthropometrics, blood pressure, blood glucose, lipid profile, serum levels of thyroid/sex hormones and erectile function evaluated by IIEF-15 questionnaire. Targeted Next Generation Sequencing (NGS) was applied to genes involved in PDE5i pharmacodynamics and pharmacokinetics. Fasting metabolic profiles of serum and urine were assessed by nuclear magnetic resonance (NMR)-based metabolomics analysis. Patients were prescribed on-demand therapy with Sildenafil oro-dispersible film and followed-up after 3 months from recruitment. Baseline data were compared with IIEF-15 score at follow-up and with the occurrence of ADR recorded by a dedicated questionnaire. Twenty-eight patients were finally included in the analysis. Serum LDL-cholesterol levels were increased in those reporting ADR (143.3 ± 13.2 mg/dl ADR vs. 133.1 ± 12.4 mg/dl No ADR; p = 0.046). NGS data showed that specific variants of PDE11A and CYP2D7 genes were more represented in drug responders (both relative risk = 2.7 [0.9–5.1]; p = 0.04). NMR-based metabolomics showed the highest association between serum LDL-cholesterol metabolites and the occurrence of ADR (Hazard ratio = 17.5; p = 0.019). The association between lipid profile and the ADR pattern suggests major cues in the tailoring of ED therapy with PDE5i.
Efficacy and Safety of Sophora flavescens (Kushen) Based Traditional Chinese Medicine in the Treatment of Ulcerative Colitis: Clinical Evidence and Potential Mechanisms
Authors: Chen Mingjun , Ding Yuxuan , Tong Zhanqi 
Published: December 2020

Background: Radix Sophorae flavescentis (Kushen), a Chinese herb, is widely used in the treatment of ulcerative colitis (UC) with damp-heat accumulation syndrome (DHAS) according to traditional Chinese medicine (TCM) theory.Objective: The aim of this study was to illuminate the clinical efficacy and potential mechanisms of Kushen-based TCM formulations in the treatment of UC with DHAS.Materials and Methods: A systematic literature search was performed in the PubMed, EMBASE, Chinese Biomedical Literature database, China National Knowledge Infrastructure database, Chongqing VIP Information database, and Wanfang database for articles published between January 2000 and July 2020 on randomized controlled trials (RCTs) that used Kushen-based TCM formulations in the treatment of UC with DHAS. A network pharmacology approach was conducted to detect the potential pathways of Kushen against UC with DHAS.Results: Eight RCTs with a total of 983 subjects were included in the meta-analysis. Compared with the control subjects (5-aminosalicylic acid therapy), those who received Kushen-based TCM formulations for the treatment of UC showed a significantly higher clinical remission rate (RR = 1.20, 95% CI: [1.04, 1.38], p = 0.02) and lower incidence of adverse events (RR = 0.63, 95% CI [0.39, 1.01], p = 0.06). A component-target-pathway network was constructed, indicating five main components (quercetin, luteolin, matrine, formononetin, and phaseolin), three major targets (Interleukin-6, Myc proto-oncogene protein, and G1/S-specific cyclin-D1) and one key potential therapeutic pathway (PI3K-Akt signaling) of Kushen against UC with DHAS.Conclusion: Kushen-based TCM formulations provide good efficacy and possess great potential in the treatment of UC. Large-scale and high-quality clinical trials and experimental verification should be considered for further confirmation of the efficacy of Kushen-based formulations.
IL-6 but Not TNFα Levels Are Associated With Time to Pregnancy in Female Rheumatoid Arthritis Patients With a Wish to Conceive
Authors: Bongenaar Margot , Smeele Hieronymus T. W. , Lubberts Erik , Dolhain Radboud J. E. M. 
Published: December 2020

Fertility issues are common amongst women with rheumatoid arthritis (RA). Interleukin 6 (IL-6) and tumor necrosis factor alpha (TNFα), known key players in RA pathogenesis, have been associated with reproductive disorders. This study investigates the role of these cytokines in decreased fertility in women with active RA. Preconception cytokine measurements of 61 patients from the PARA-cohort, a prospective study on RA and pregnancy, were studied in relation to time to pregnancy as a measure for fertility. IL-6 levels were higher in patients with a time to pregnancy longer than 1 year (p= 0.016). Survival analysis of patients stratified by high or low serum IL-6 levels, shows a prolonged time to pregnancy in the high IL-6 group (p= 0.045). Univariate cox regression analysis of IL-6 in relation to time to pregnancy as well as multivariate cox regression analysis correcting for age, disease activity, nulliparity, NSAID use and prednisone use were performed, with hazards ratios for log transformed IL-6 of 0.68 (95% CI: 0.51–0.93,p= 0.015) and 0.66 (95% CI: 0.43–0.99,p= 0.044), respectively. For TNFα, no association with time to pregnancy was found. This study shows that high IL-6, but not TNFα, is associated with decreased fertility in women with RA. This finding provides a rationale to therapeutically target the IL-6 pathway in the time period before pregnancy. More research in the form of large cohort studies on drug safety and the effect of bDMARDS on fertility is needed for implementation of treatment strategies directed at fertility issues in women with RA.
Effect of Combined Electroacupuncture and Selegiline Treatment in Alzheimer’s Disease: An Animal Model
Authors: Cai Mudan , Yang Eun Jin 
Published: December 2020

The complexity of pathological mechanisms in Alzheimer’s disease (AD) poses significant challenges to the development of corresponding drugs. Symptom-specific pharmacological interventions and alternative treatments provide promising treatment possibilities. Therefore, we considered a combination of selegiline (SEL) and electroacupuncture (EA). We used an animal model with AD to investigate the effect of a combination of these treatments on cognitive function. 5XFAD mice received a week of SEL treatment and 2 weeks of EA. Novel object recognition and Y-maze tests were subsequently performed to assess their cognitive functions. To determine the molecular action of the combination treatment, Western blots, Aβ1-42 enzyme-linked immunosorbent assays (ELISA), and micro-positron-emission tomography were also performed to assess pathological markers and processes. The results were assessed based on the difference between untreated transgenic, SEL-treated, and SEL- and EA-treated groups of mice. Mice in the combined treatment group demonstrated significantly better cognitive functions, and lesser neuroinflammation than the comparative groups. In addition, mice treated with a combination of SEL and EA did not demonstrate a direct modulation of insoluble Aβ but demonstrated greater glucose metabolism. Our findings demonstrated that SEL combined with EA treatment was associated with better cognitive functioning due to inhibition of neuroinflammation and increased glucose metabolism relative to the comparative groups in a mouse model with AD.