bluuebunny/crossref_metadata_2025_split huggingface dataset:
IDENTIFYING DIGITAL BIOMARKERS OF DEMENTIA-RELATED AGITATION: PRELIMINARY RESULTS FROM THE MODERATE STUDY
Authors: Au-Yeung Wan-Tai M , Miller Lyndsey M , Beattie Zachary , Reynolds Christina L , Kaye Jeffrey 
Published: April 2020

Abstract
Agitation is a challenging dementia-related behavioral symptom and disconcertingly leads to an undesirable increase in pharmaceutical intervention. Agitation is primarily assessed subjectively by time-impoverished caregivers or clinicians, who may not be able to provide comprehensive or sensitive enough reporting to detect early signs of agitation or identify its precipitants. The purpose of the MODERATE (Monitoring Dementia Related Agitation Using Technology Evaluation) study is to characterize dementia-related agitation and its potential catalysts using wearables and sensors installed in participants’ private living quarters within memory care (skilled nursing) facilities. Preliminary data from seven episodes of agitation–reported by nursing staff and treated by pharmaceutical intervention–were captured and characterized with an integrated network consisting of research-grade actigraphy, passive infrared presence sensors, door contact sensors, ambient environmental sensor boards, and bed sensor mats. Behaviors (e.g. pacing, space transitions) during 8-hour nursing shifts that included episodes of agitation were compared with 8-hour nursing shifts without any episodes of agitation. The environmental conditions (temperature, lighting, humidity, and decibel level) during agitated periods and non-agitated periods were also compared. There was a statistically significant difference between agitated periods and non-agitated periods for the number of space transitions in the living room (p&lt;0.01) and for the measured amount of ambient light and humidity (p &lt;0.05). Passive monitoring of behavioral activity is a promising method of unobtrusive, early, and objective detection of dementia-related agitation, expanding the window of opportunity for identifying factors associated with behavioral change and delivering appropriate evidence-based interventions.
Mechanical Ecology—Taking Biomechanics to the Field
Authors: Bauer Ulrike , Poppinga Simon , Müller Ulrike K 
Published: April 2020

AbstractSynopsis Interdisciplinary research can have strong and surprising synergistic effects, leading to rapid knowledge gains. Equally important, it can help to reintegrate fragmented fields across increasingly isolated specialist sub-disciplines. However, the lack of a common identifier for research “in between fields” can make it difficult to find relevant research outputs and network effectively. We illustrate and address this issue for the emerging interdisciplinary hotspot of “mechanical ecology,” which we define here as the intersection of quantitative biomechanics and field ecology at the organism level. We show that an integrative approach crucially advances our understanding in both disciplines by (1) putting biomechanical mechanisms into a biologically meaningful ecological context and (2) addressing the largely neglected influence of mechanical factors in organismal and behavioral ecology. We call for the foundation of knowledge exchange platforms such as meeting symposia, special issues in journals, and focus groups dedicated to mechanical ecology.
Aminoglycosides are efficient reagents to induce readthrough of premature termination codon in mutant B4GALNT1 genes found in families of hereditary spastic paraplegia
Authors: Yesmin Farhana , Bhuiyan Robiul H , Ohmi Yuhsuke , Ohkawa Yuki , Tajima Orie , Okajima Tetsuya , Furukawa Keiko , Furukawa Koichi 
Published: April 2020

AbstractThe readthrough of premature termination codon (PTC) by ribosome sometimes produces full-length proteins. We previously reported a readthrough of PTC of glycosyltransferase gene B4GALNT1 with hereditary spastic paraplegia (HSP). Here we featured the readthrough of B4GALNT1 of two mutants, M4 and M2 with PTC by immunoblotting and flow cytometry after transfection of B4GALNT1 cDNAs into cells. Immunoblotting showed a faint band of full-length mutant protein of M4 but not M2 at a similar position with that of wild-type B4GALNT1. AGC sequences at immediately before and after the PTC in M4 were critical for the readthrough. Treatment of cells transfected with mutant M4 cDNA with aminoglycosides resulted in increased readthrough of PTC. Furthermore, treatment of transfectants of mutant M2 cDNA with G418 also resulted in the induction of readthrough of PTC. Both M4 and M2 cDNA transfectants showed increased/induced bands in immunoblotting and GM2 expression in a dose-dependent manner of aminoglycosides. Results of mass spectrometry supported this effect. Here, we showed for the first time the induction and/or enhancement of the readthrough of PTCs of B4GALNT1 by aminoglycoside treatment, suggesting that aminoglycosides are efficient for patients with HSP caused by PTC of B4GALNT1, in which gradual neurological disorders emerged with aging.
Convenient method of producing cyclic single-chain Fv antibodies by split-intein-mediated protein ligation and chaperone co-expression
Authors: Liu Chenjiang , Kobashigawa Yoshihiro , Yamauchi Soichiro , Fukuda Natsuki , Sato Takashi , Masuda Takeshi , Ohtsuki Sumio , Morioka Hiroshi 
Published: April 2020

Abstract
Single-chain Fv (scFv) is a recombinant antibody in which the variable regions of the heavy chain (VH) and light chain (VL) are connected by a short flexible polypeptide linker. Compared with monoclonal antibodies, scFvs have the advantages of low-cost production using Escherichia coli and easy genetic manipulation. ScFvs are, therefore, regarded as useful modules for producing next-generation medical antibodies. The practical use of scFvs has been limited due to their aggregation propensity mediated by interchain VH–VL interactions. To overcome this problem, we recently reported a cyclic scFv whose N-terminus and C-terminus were connected by sortase A-mediated ligation. Preparation of cyclic scFv is, however, a time-consuming process. To accelerate the application study of cyclic scFv, we developed a method to produce cyclic scFv by the combined use of a protein ligation technique based on protein trans-splicing reaction (PTS) by split intein and a chaperone co-expression system. This method allows for the preparation of active cyclic scFv from the cytoplasm of E. coli. The present method was applied to the production of cyclic 73MuL9-scFv, a GA-pyridine antibody, as a kind of advanced glycation end-product. These findings are expected to evoke further application study of cyclic scFv.
Preserving Vision: Rethinking Burn Patient Monitoring to Prevent Orbital Compartment Syndrome
Authors: Mai Anthony P , Fortenbach Christopher R , Wibbenmeyer Lucy A , Wang Kai , Shriver Erin M 
Published: April 2020

Abstract
Burn patients receiving aggressive fluid resuscitation are at risk of developing orbital compartment syndrome (OCS). This condition results in elevated orbital pressures and can lead to rapid permanent vision loss. Risk factors and monitoring frequency for OCS remain largely unknown. A retrospective review was therefore conducted of admitted burn patients evaluated by the ophthalmology service at an American Burn Association verified Burn Treatment Center. Demographic, burn, examination, and fluid resuscitation data were compared using two-sided t-tests, Fisher’s exact tests, and linear regression. Risk factors for elevated intraocular pressures (IOPs; a surrogate for intraorbital pressure) in patients resuscitated via the Parkland formula were found to be total body surface area (% TBSA) burned, resuscitation above the Ivy Index (&gt;250 ml/kg), and Parkland formula calculated volume. Maximum IOP and actual fluid resuscitation volume were linearly related. Analysis of all patients with elevated IOP found multiple patients with significant IOP increases after initial evaluation resulting in OCS within the first 24 hours postinjury. While %TBSA, Ivy Index, and resuscitation calculated volume are OCS risk factors in burn patients, two patients with facial burns developed OCS (25% of all patients with OCS) despite not requiring resuscitation. Orbital congestion can develop within the first 24 hours of admission when resuscitation volumes are the greatest. In addition to earlier and more frequent IOP checks in susceptible burn patients during the first day, the associated risk factors will help identify those most at risk for OCS and vision loss.
Education for Humanity: higher education for refugees in resource-constrained environments through innovative technology
Authors: Bauer Carrie , Gallagher Matthew J 
Published: April 2020

AbstractHalf of refugee children attend primary school and 22 per cent attend secondary school, yet only 3 per cent have access to higher education. When higher education efforts do exist, they often ignore common barriers refugees face in accessing it: cost, connectivity, lack of a power source, and access to devices, among others. Arizona State University’s Education for Humanity team piloted a programme to address this lack of access and associated barriers. Using a solar-powered, offline technology that emits a Wi-Fi hotspot, the team implemented a university-level course in Nakivale Settlement, Uganda. This article presents the results and findings from this pilot programme.
Fundamental differences in the radio properties of red and blue quasars: enhanced compact AGN emission in red quasars
Authors: Fawcett V A , Alexander D M , Rosario D J , Klindt L , Fotopoulou S , Lusso E , Morabito L K , Calistro Rivera G 
Published: April 2020

ABSTRACT
We have recently used the Faint Images of the Radio Sky at Twenty-centimeters (FIRST) survey to show that red quasars have fundamentally different radio properties to typical blue quasars: a significant (factor ≈3) enhancement in the radio-detection fraction, which arises from systems around the radio-quiet threshold with compact (&lt;5 arcsec) radio morphologies. To gain greater insight into these physical differences, here we use the DR14 Sloan Digital Sky Survey (SDSS) and more sensitive, higher resolution radio data from the Very Large Array (VLA) Stripe 82 (S82) and VLA-COSMOS 3 GHz (C3GHz) surveys. With the S82 data, we perform morphological analyses at a resolution and depth three times that of the FIRST radio survey, and confirm an enhancement in radio-faint and compact red quasars over typical quasars; we now also find tentative evidence for an enhancement in red quasars with slightly extended radio structures (16–43 kpc at z = 1.5). These analyses are complemented by C3GHz, which is deep enough to detect radio emission from star-formation processes. From our data we find that the radio enhancement from red quasars is due to AGN activity on compact scales (≲43 kpc) for radio-intermediate–radio-quiet sources (−5 &lt; $\mathcal {R}$ &lt; −3.4, where $\mathcal {R}$ = $L_{\rm{1.4\,GHz}}/L_{6\mu\text{m}}$), which decreases at $\mathcal {R}$ &lt; −5 as the radio emission from star-formation starts to dilute the AGN component. Overall our results argue against a simple orientation scenario and are consistent with red quasars representing a younger, earlier phase in the overall evolution of quasars.
ATAD5 deficiency alters DNA damage metabolism and sensitizes cells to PARP inhibition
Authors: Giovannini Sara , Weller Marie-Christine , Hanzlíková Hana , Shiota Tetsuya , Takeda Shunichi , Jiricny Josef 
Published: April 2020

AbstractReplication factor C (RFC), a heteropentamer of RFC1-5, loads PCNA onto DNA during replication and repair. Once DNA synthesis has ceased, PCNA must be unloaded. Recent findings assign the uloader role primarily to an RFC-like (RLC) complex, in which the largest RFC subunit, RFC1, has been replaced with ATAD5 (ELG1 in Saccharomyces cerevisiae). ATAD5-RLC appears to be indispensable, given that Atad5 knock-out leads to embryonic lethality. In order to learn how the retention of PCNA on DNA might interfere with normal DNA metabolism, we studied the response of ATAD5-depleted cells to several genotoxic agents. We show that ATAD5 deficiency leads to hypersensitivity to methyl methanesulphonate (MMS), camptothecin (CPT) and mitomycin C (MMC), agents that hinder the progression of replication forks. We further show that ATAD5-depleted cells are sensitive to poly(ADP)ribose polymerase (PARP) inhibitors and that the processing of spontaneous oxidative DNA damage contributes towards this sensitivity. We posit that PCNA molecules trapped on DNA interfere with the correct metabolism of arrested replication forks, phenotype reminiscent of defective homologous recombination (HR). As Atad5 heterozygous mice are cancer-prone and as ATAD5 mutations have been identified in breast and endometrial cancers, our finding may open a path towards the therapy of these tumours.
Analgesic Effectiveness of Perioperative Ultrasound-Guided Serratus Anterior Plane Block Combined with General Anesthesia in Patients Undergoing Video-Assisted Thoracoscopic Surgery: A Systematic Review and Meta-analysis
Authors: Zhang Xiaofei , Zhang Chao , Zhou Xiaofeng , Chen Wei , Li Junhong , Wang Huan , Liu Juying 
Published: April 2020

AbstractObjectiveTo investigate whether perioperative ultrasound-guided serratus anterior plane block (SAPB) combined with general anesthesia is more effective and safer than current analgesic techniques for postoperative analgesia after video-assisted thoracoscopic surgery (VATS).MethodsPubMed, the Cochrane Library, and EMBASE were searched for clinical trials published up to July 31, 2019. Outcomes, including operative duration, postoperative pain scores, postoperative analgesia use, patient satisfaction with analgesia, time to chest tube removal, length of stay, and adverse effects were analyzed.ResultsFour clinical trials, including 262 patients, met inclusion criteria. Ultrasound-guided SAPB reduced pain scores at zero, 15, 30, 45, and 60 minutes in the postoperative anesthesia care unit (all P &lt; 0.05) and at one, two, six, 12, and 24 hours in the ward (all P &lt; 0.001). Additionally, postoperatively, morphine consumption at 15 and 30 minutes, overall morphine consumption, and total consumption (morphine plus tramadol) were significantly lower in the SAPB cohort (P  &lt; 0.05). Similarly, postoperative tramadol consumption at one, two, six, 12, and 24 hours was also lower in this cohort (all P  &lt; 0.05). The postoperative consumption of fentanyl, tramadol, and total morphine in patient-controlled analgesia (PCA) at 24 hours was significantly reduced (P &lt; 0.05). Moreover, SAPB provided better patient satisfaction with analgesia (P = 0.0038). However, no statistically significant difference was found in duration of operation, time to chest tube removal, length of stay, or side effects (all P &gt; 0.05).ConclusionsPerioperative ultrasound-guided SAPB combined with general anesthesia provided more effective postoperative analgesia after VATS. However, no significant advantage was found regarding side effects.
Pain Management Best Practices from Multispecialty Organizations During the COVID-19 Pandemic and Public Health Crises
Authors: Cohen Steven P , Baber Zafeer B , Buvanendran Asokumar , McLean Brian C , Chen Yian , Hooten W Michael , Laker Scott R , Wasan Ajay D , Kennedy David J , Sandbrink Friedhelm , King Scott A , Fowler Ian M , Stojanovic Milan P , Hayek Salim M , Phillips Christopher R 
Published: April 2020

AbstractBackgroundIt is nearly impossible to overestimate the burden of chronic pain, which is associated with enormous personal and socioeconomic costs. Chronic pain is the leading cause of disability in the world, is associated with multiple psychiatric comorbidities, and has been causally linked to the opioid crisis. Access to pain treatment has been called a fundamental human right by numerous organizations. The current COVID-19 pandemic has strained medical resources, creating a dilemma for physicians charged with the responsibility to limit spread of the contagion and to treat the patients they are entrusted to care for.MethodsTo address these issues, an expert panel was convened that included pain management experts from the military, Veterans Health Administration, and academia. Endorsement from stakeholder societies was sought upon completion of the document within a one-week period.ResultsIn these guidelines, we provide a framework for pain practitioners and institutions to balance the often-conflicting goals of risk mitigation for health care providers, risk mitigation for patients, conservation of resources, and access to pain management services. Specific issues discussed include general and intervention-specific risk mitigation, patient flow issues and staffing plans, telemedicine options, triaging recommendations, strategies to reduce psychological sequelae in health care providers, and resource utilization.ConclusionsThe COVID-19 public health crisis has strained health care systems, creating a conundrum for patients, pain medicine practitioners, hospital leaders, and regulatory officials. Although this document provides a framework for pain management services, systems-wide and individual decisions must take into account clinical considerations, regional health conditions, government and hospital directives, resource availability, and the welfare of health care providers.
Home and Community-Based Physical Therapist Management of Adults With Post–Intensive Care Syndrome
Authors: Smith James M , Lee Alan C , Zeleznik Hallie , Coffey Scott Jacqueline P , Fatima Arooj , Needham Dale M , Ohtake Patricia J 
Published: April 2020

Abstract
More than 4 million adults survive a stay in the intensive care unit each year, with many experiencing new or worsening physical disability, mental health problems, and/or cognitive impairments, known as post-intensive care syndrome (PICS). Given the prevalence and magnitude of physical impairments after critical illness, many survivors, including those recovering from COVID-19, could benefit from physical therapist services after hospital discharge. However, due to the relatively recent recognition and characterization of PICS, there may be limited awareness and understanding of PICS among physical therapists practicing in home health care and community-based settings. This lack of awareness may lead to inappropriate and/or inadequate rehabilitation service provision. While this perspective article provides information relevant to all physical therapists, it is aimed toward those providing rehabilitation services outside of the acute and postacute inpatient settings. This article reports the prevalence and clinical presentation of PICS and provides recommendations for physical examination and outcomes measures, plan of care, and intervention strategies. The importance of providing patient and family education, coordinating community resources including referring to other health care team members, and community-based rehabilitation service options is emphasized. Finally, this perspective article discusses current challenges for optimizing outcomes for people with PICS and suggests future directions for research and practice.
Beneficial effect of Mediterranean diet on disease activity and cardiovascular risk in systemic lupus erythematosus patients: a cross-sectional study
Authors: Pocovi-Gerardino Gabriela , Correa-Rodríguez María , Callejas-Rubio José-Luis , Ríos-Fernández Raquel , Martín-Amada María , Cruz-Caparros María-Gracia , Rueda-Medina Blanca , Ortego-Centeno Norberto 
Published: April 2020

Abstract

Objective
To analyse the influence of the Mediterranean diet (Med Diet) on SLE activity, damage accrual and cardiovascular disease risk markers.

Methods
A cross-sectional study was conducted on 280 patients with SLE [46.9 (12.85) years]. Med Diet adherence was assessed through a 14-item questionnaire on food consumption frequency and habits (total score from 0 to 14 points; higher score is greater adherence to the Med Diet). CRP, homocysteine, SLEDAI-2K (SLE disease activity), and SLICC/ACR and SDI (damage accrual) were measured. Obesity, diabetes mellitus, hypertension and blood lipids, among others, were considered cardiovascular disease risk factors.

Results
Greater adherence to the Med Diet was significantly associated with better anthropometric profiles, fewer cardiovascular disease risk factors, and lower disease activity and damage accrual scores (P ≤ 0.001 for SLEDAI and SDI). An inverse relationship between the Med Diet score and SLEDAI (P ≥ 0.001; β = −0.380), SDI (P ≤ 0.001; β = −0.740) and hsCRP (P = 0.039; β = −0.055) was observed. The odds ratio for having active SLE (SLEDAI ≥5) or the presence of damage (SDI ≥1) was lower among patients whose Med Diet score was higher (P ≤ 0.001). Finally, greater consumption of Med Diet foods (olive oil, fruits, vegetables, fish, etc.) and abstaining from red meat and meat products, sugars and pastries was associated with less SLE clinical activity and damage.

Conclusion
Greater adherence to the Med Diet seems to exert a beneficial effect on disease activity and cardiovascular risk in SLE patients. To confirm these findings, further longitudinal studies would be of interest.
Diagnosing REM sleep behavior disorder in Parkinson’s disease without a gold standard: a latent-class model study
Authors: Figorilli Michela , Marques Ana R , Meloni Mario , Zibetti Maurizio , Pereira Bruno , Lambert Celine , Puligheddu Monica , Cicolin Alessandro , Lopiano Leonardo , Durif Franck , Fantini Maria L 
Published: April 2020

Abstract

Study Objectives
To ascertain whether current diagnostic criteria for REM sleep behavior disorder (RBD) are appropriate in patients with Parkinson’s disease (PD) consulting a movement disorder center, to evaluate the accuracy of REM sleep without atonia (RSWA) thresholds and determine the value of screening questionnaires to discriminate PD patients with RBD.

Methods
One hundred twenty-eight consecutive PD patients (M = 80; mean age: 65.6 ± 8.3 years) underwent screening questionnaires, followed by a sleep-focused interview and a full-night video-polysomnography (vPSG). Without a gold standard, latent class models (LCMs) were applied to create an unobserved (“latent”) variable. Sensitivity analysis was performed using RSWA cutoff derived from two visual scoring methods. Finally, we assessed the respective diagnostic performance of each diagnostic criterion for RBD and of the screening questionnaires.

Results
According to the best LCM-derived model, patients having either “history” or “video” with RSWA or alternatively showing both “history” and “video” without RSWA were classified as having RBD. Using both SINBAR and Montreal scoring methods, RSWA criterion showed the highest sensitivity while concomitant history of RBD and vPSG-documented behaviors, regardless to presence of RSWA, displayed the highest specificity. Currently recommended diagnostic threshold of RSWA was found to be optimal in our large cohort of PD patients. Both the RBD screening questionnaire (RBDSQ) and the RBD single question (RBD1Q) showed poor sensitivity and specificity.

Conclusions
Results of the best LCM for diagnosis of RBD in PD were consistent with the current diagnostic criteria. Moreover, RBD might be considered in those PD patients with both history and vPSG-documented dream enactment behaviors, but with RSWA values within the normal range.