bluuebunny/crossref_metadata_2025_split huggingface dataset:
Postoperative adjuvant therapy following radical resection for intrahepatic cholangiocarcinoma: A multicenter retrospective study
Authors: Wang Lei , Deng Manjun , Ke Qiao , Lou Jianying , Zheng Shuguo , Bi Xinyu , Wang Jianming , Guo Wei , Li Fuyu , Wang Jian , Zheng Yamin , Li Jingdong , Cheng Shi , Zhou Weiping , Zeng Yongyi 
Published: February 2020

AbstractBackground and AimsThe prognosis of intrahepatic cholangiocarcinoma (ICC) after radical resection is far from satisfactory; however, the clinical value of adjuvant therapy (AT) remains controversial. This multicenter study aimed to evaluate the clinical value of AT and identify potential patients who would be benefited from AT.MethodsData from ICC patients who underwent radical resection were retrospectively collected from 12 hepatobiliary centers in China between December 2012 and December 2015. Patients were divided into AT and non‐AT groups based on whether AT was administered or not. Overall survival (OS) and disease‐free survival (DFS) were analyzed using the Kaplan‐Meier method before and after 1:2 propensity score matching (PSM). Subgroup analyses were conducted based on the established staging systems.ResultsA total of 412 patients were enrolled in this study, and 77 patients (18.9%) received AT, including 32 (7.8%) patients who received transarterial chemoembolization (TACE), 21 (5.1%) patients who received chemotherapy, 10 (2.4%) patients who received radiotherapy, and 14 (3.4%) patients who received adjuvant chemoradiotherapy. The median OS and DFS were both longer in the AT group than in the non‐AT group (43.0 months vs 21.0 months, P = .015; 16.0 months vs 11.0 months, P = .045, respectively), and the advantage of AT was confirmed for both the OS and DFS (P = .023; P = .046, respectively) after 1:2 PSM. Furthermore, based on the established nomogram, only “middle‐risk” patients receiving AT cherished a longer median OS (43.0 months vs 20.0 months, P = .033). In subgroup analyses that were stratified by different AT strategies, patients receiving postoperative chemotherapy had a longer median OS (37.0 months vs 21.0 months, P = .039), while patients receiving postoperative TACE had a longer median DFS (50.0 months vs 11.0 months, P = .007).ConclusionWith the current data, we conclude that AT benefits ICC patients following radical resection, especially those “middle‐risk” patients, as evaluated by the established nomogram. However, exactly which patients are the most suitable for AT requires further study and validation.
Utility of the long DrySeal sheath in facilitating transcatheter pulmonary valve implantation with the Edwards Sapien 3 valve
Authors: Fukuda Terunobu , Tan Weiyi , Sadeghi Soraya , Lin Jeannette , Salem Morris , Levi Daniel , Aboulhosn Jamil 
Published: February 2020

AbstractObjectiveTo assess the utility of the 65‐cm‐long Gore DrySeal sheath when compared to the standard 36‐cm‐long Edwards expandable sheath (e‐sheath) for transcatheter pulmonary valve implantation (TPVI) with the Edwards Sapien 3 valve.MethodsAll patients who underwent TPVI with the Sapien 3 valve, excluding those performed via hybrid approach, at our center between September 2015 and November 2019 were retrospectively reviewed and compared between two groups.ResultsA total of 94 patients were enrolled; 29 patients underwent TPVI with the Sapien valve using the DrySeal sheath and 65 underwent TPVI using the e‐sheath. The height and body weight of patients implanted using the DrySeal sheath ranged from 137 to 193 cm and from 33 to 129 kg, respectively. Valve delivery time was significantly shorter in the DrySeal group (median time 4 min 33 s vs. 9 min 6 s, p = .002). There were no complications in the DrySeal group (0/27). Nine procedural complications occurred in the e‐sheath group (9/65), five of which were potentially directly related to sheath choice, including tricuspid valve injury in four and embolization of the tip of the e‐sheath during retrieval of a ruptured balloon in one patient.ConclusionsTPVI with the Sapien 3 valve using the 65‐cm‐long DrySeal sheath facilitates faster and safer valve implantation when compared to the e‐sheath.
Percutaneous pulmonary valve implantation in a dysfunctional Trifecta® bioprothesis after high‐pressure balloon fracturing
Authors: Langhammer Florian , Lehner Anja , Haas Nikolaus A. , Jakob André 
Published: February 2020

AbstractA percutaneous pulmonary valve‐in‐valve (PPVIV) implantation in small surgical tissue valves may be limited due to the valve's initial diameter. Fracturing of the valve's integrity by high‐pressure balloons may enhance the diameter and facilitate subsequent PPVIV with a large valve. To the best of our knowledge, the Trifecta® valve seemed not to be accessible for fracturing. We report a case of successful 19‐mm Trifecta valve fracturing, followed by PPVIV using a 26‐mm Edwards SAPIEN 3 valve in pulmonary position. By repetitively using a high‐pressure balloon 5 mm larger than the labeled valve size, we were able to fracture the valve's integrity and implant a 26‐mm valve thereafter. Therefore, Trifecta valve appears to be suitable for valve ring fracturing and subsequent PPVIV in certain patients.
One‐Pot Substitution of Aliphatic Alcohols Mediated by Sulfuryl Fluoride
Authors: Mo Jia Yi , Epifanov Maxim , Hodgson Jack W. , Dubois Rudy , Sammis Glenn M. 
Published: February 2020

AbstractThe Mitsunobu reaction is a powerful transformation for the one‐pot activation and substitution of aliphatic alcohols. Significant efforts have focused on modifying the classic conditions to overcome problems associated with purification from phosphine‐based byproducts. Herein, we report a phosphine free method for alcohol activation and substitution that is mediated by sulfuryl fluoride. This new method is effective for a wide range of primary alcohols using phthalimide, di‐tert‐butyl‐iminodicarboxylate, and aromatic thiol nucleophiles in 74 % average yield. Activated carbon nucleophiles and a deactivated phenol were also effective for this reaction in good yields. Secondary alcohols were also successful substrates using aryl thiols, affording the corresponding sulfides in 56 % average yield with enantiomeric ratios up to 99:1. This new protocol has a distinct synthetic advantage over many existing phosphine‐based methods as the byproducts are readily separable. This feature was exploited in several examples that did not require chromatography for purification. Furthermore, the mild reaction conditions enabled further in situ derivatization for the one‐pot conversion of alcohols to amines or sulfones. This method also provides a boarder nucleophile scope compared to existing phosphine‐free methods.
Le comportement d'achat organisationnel des emballages des produits périssables dans le secteur du commerce de détail
Authors: Afif Karima , Rebolledo Claudia , Roy Jacques 
Published: February 2020

AbstractThis qualitative study investigates the structure of the buying center, the buying process and the factors that influence the buying decision of perishable products packaging. This empirical investigation is based on the case study of a major Quebec food retailer. We show that the purchase of packaging changes significantly depending on whether the product is considered more or less strategic for the organization, which determines the required level of packaging customization. Buying center structure becomes more complex as packaging customization increases. Given the multifunctionality and the interdisciplinarity of packaging, we propose a multi‐criteria analysis grid to improve buying decision efficiency and streamline communication between the various levels of the buying center.
Studying Nicotinic Acetylcholine Receptors Using the IonFlux™ Microfluidic‐Based Automated Patch‐Clamp System with Continuous Perfusion and Fast Solution Exchange
Authors: Yehia Ali , Wei Haiyang 
Published: February 2020

AbstractAutomated patch‐clamp (APC) systems have become indispensable tools of drug‐discovery programs by allowing high‐throughput electrophysiology‐based screening of ion channel compounds. The recent development and introduction of microfluidics‐based APC systems have made it possible to study the interactions of ligand‐gated ion channels with pharmacological reagents, such as agonists, antagonists, or positive allosteric modulators (PAMs), with reliable pharmacological results comparable to those of the gold‐standard manual patch‐clamp technique while maintaining high‐throughput capacity. Many ligand‐gated ion channels exhibit rapid desensitization upon repetitive introduction of ligands; this loss of channel activity in the absence of pharmacological interaction poses a challenge for developing accurate, precise, and robust assays with high success rate, low run‐down, and reliable pharmacological results. Here we present procedures to study nicotinic acetylcholine receptors (nAChRs) with the IonFlux™, an automated patch‐clamp system with continuous flow and precise fluidic exchange; these procedures can also be generalized to the study of other ligand‐gated ion channels. We present protocols to study agonist, antagonist, and PAM activities on nAChRs, particularly the rapidly desensitizing nAChR α7 receptors. The data demonstrate that the IonFlux™ system is a fast, robust, and reliable platform for the study of nAChRs and other ligand‐gated ion channels, generating data that closely mimic those from manual patch‐clamp conditions. © 2020 by John Wiley & Sons, Inc.Basic Protocol 1: Measuring agonist concentration‐dependent responseBasic Protocol 2: Measuring antagonist concentration‐dependent responseBasic Protocol 3: Measuring positive allosteric modulator (PAM) concentration‐dependent responseSupport Protocol 1: Basic IonFlux system operationSupport Protocol 2: Plate care and fillingSupport Protocol 3: Plate preparation for water rinsingSupport Protocol 4: Water rinsing of platesSupport Protocol 5: Plate primingSupport Protocol 6: General assaySupport Protocol 7: Editing the compound addition sequence (compound list)Support Protocol 8: Creating compound list for agonist concentration‐dependent responseSupport Protocol 9: Creating compound list for antagonist or PAM concentration‐dependent responseSupport Protocol 10: Defining the different compounds used or compound listSupport Protocol 11: MaintenanceSupport Protocol 12: Data analysisSupport Protocol 13: Cell culture
Natural killer cell activation by respiratory syncytial virus‐specific antibodies is decreased in infants with severe respiratory infections and correlates with Fc‐glycosylation
Authors: van Erp Elisabeth A , Lakerveld Anke J , de Graaf Erik , Larsen Mads D , Schepp Rutger M , Hipgrave Ederveen Agnes L , Ahout Inge ML , de Haan Cornelis AM , Wuhrer Manfred , Luytjes Willem , Ferwerda Gerben , Vidarsson Gestur , van Kasteren Puck B 
Published: February 2020

AbstractObjectivesRespiratory syncytial virus (RSV) is a major cause of severe lower respiratory tract infections in infants, and there is no vaccine available. In early life, the most important contributors to protection against infectious diseases are the innate immune response and maternal antibodies. However, antibody‐mediated protection against RSV disease is incompletely understood, as both antibody levels and neutralisation capacity correlate poorly with protection. Since antibodies also mediate natural killer (NK) cell activation, we investigated whether this functionality correlates with RSV disease.MethodsWe performed an observational case–control study including infants hospitalised for RSV infection, hernia surgery or RSV‐negative respiratory viral infections. We determined RSV antigen‐specific antibody levels in plasma using a multiplex immunoassay. Subsequently, we measured the capacity of these antibodies to activate NK cells. Finally, we assessed Fc‐glycosylation of the RSV‐specific antibodies by mass spectrometry.ResultsWe found that RSV‐specific maternal antibodies activate NK cells in vitro. While concentrations of RSV‐specific antibodies did not differ between cases and controls, antibodies from infants hospitalised for severe respiratory infections (RSV and/or other) induced significantly less NK cell interferon‐γ production than those from uninfected controls. Furthermore, NK cell activation correlated with Fc‐fucosylation of RSV‐specific antibodies, but their glycosylation status did not significantly differ between cases and controls.ConclusionOur results suggest that Fc‐dependent antibody function and quality, exemplified by NK cell activation and glycosylation, contribute to protection against severe RSV disease and warrant further studies to evaluate the potential of using these properties to evaluate and improve the efficacy of novel vaccines.
Appropriate buffer sizes for Internet nodal devices: A networks' topologies–based solution
Authors: Eyinagho Monday Ofori , Falaki Samuel Oluwole 
Published: February 2020

SummarySwitched networks' (including the Internet) nodal devices require buffers to hold packets during periods of congestion and when traffic streams on more than one input port need to access the same output port. Appropriately sizing the buffers has been a contentious issue among researchers over the last couple of years. Three main buffer sizing formulas have been reported in literature, which are: bandwidth‐delay product or BDP, small buffer, and the tiny buffer formulas. But researchers are largely agreed that the BDP formula results in unrealistically large and wasteful buffers, while a number of researchers have held with good reasons, that the small and tiny buffer formulas are theoretical formulations that cannot be generally and practically applied to real networks. Some researchers have even asserted that deriving a single, universal formula for sizing buffers may not be possible. The main purpose of this paper is to explain an approach, which we term “network topology point –of –view,” that we developed to derive a novel, empirical, generic, practical, easy‐to‐apply, closed‐form formula that can be used to obtain the buffer capacity that is optimal—in terms of the minimum capacity possible for lossless operation, for any switched network node, including nodes (switches and routers) in the Internet. The results obtained from utilizing the formula to specify typical nodal buffers' capacities are largely in agreement with the values of the tiny buffer formula in literature; but this paper's formula additionally specifies what we term “very tiny buffers.” The simple approach adopted for presenting this paper makes it a handy tutorial material that explains this “network topology point‐of‐view” concept.
Microglia in the developing retina couple phagocytosis with the progression of apoptosis via P2RY12 signaling
Authors: Blume Zachary I. , Lambert Jared M. , Lovel Anna G. , Mitchell Diana M. 
Published: February 2020

AbstractBackgroundMicroglia colonize the developing vertebrate central nervous system coincident with the detection of developmental apoptosis. Our understanding of apoptosis in intact tissue in relation to microglial clearance of dying cells is largely based on fixed samples, which is limiting given that microglia are highly motile and mobile phagocytes. Here, we used a system of microglial depletion and in vivo real‐time imaging in zebrafish to directly address microglial phagocytosis of apoptotic cells during normal retinal development, the relative timing of phagocytosis in relation to apoptotic progression, and the contribution of P2RY12 signaling to this process.ResultsThe depletion of microglia resulted in accumulation of numerous apoptotic cells in the retina. Real‐time imaging revealed precise timing of microglial engulfment with the progression of apoptosis, and dynamic movement and displacement of engulfed apoptotic cells. Inhibition of P2RY12 signaling delayed microglial clearance of apoptotic cells.ConclusionsMicroglial engulfment of dying cells is coincident with apoptotic progression and requires P2RY12 signaling, indicating that microglial P2RY12 signaling is shared between development and injury response. Our work provides important in vivo insight into the dynamics of apoptotic cell clearance in the developing vertebrate retina and provides a basis to understand microglial phagocytic behavior in health and disease.
The Hidden Dimension: Context-Dependent Expression of Repeatable Behavior in Copepods
Authors: Heuschele Jan , Lode Torben , Andersen Tom , Titelman Josefin 
Published: February 2020

Abstract
In ecotoxicology and aquatic ecology, we often ignore responses of individuals and focus on average responses. However, both terrestrial and aquatic animals display consistent behavioral differences between individuals. The distribution of behavioral differences within a population contains vital information for predicting population responses to novel environmental challenges. Currently, individual data for behavioral and physiological traits of small marine invertebrates are few, partly because such variation is lost within published group means and assumed normality. We tested the combined effects of an inorganic contaminant (copper) and a biological stressor (i.e., chemical cues of a fish predator) on activity in a marine copepod. Although direct stress effects were weak, individuals behaved consistently differently, depending on the context. Individual differences in behavior were only expressed under the influence of kairomones, but not by copper exposure alone. This finding indicates that copepods express repeatable and context-dependent behavior. We also demonstrate how large variations in behavioral data can hide consistent differences between individuals. Environ Toxicol Chem 2020;39:1017–1026. © 2020 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.
The Effects of 17α-Ethinylestradiol on the Heart Rate of Embryonic Japanese Medaka (Oryzias latipes)
Authors: Anderson Jordan Christopher , Beyger Lindsay , Guchardi John , Holdway Douglas Alan 
Published: February 2020

Abstract
Estrogen toxicity has been an area of priority in aquatic toxicology over the last 20 yr. Currently, estrogen toxicity is primarily linked to classical estrogen signaling, the interaction of estrogen receptors alpha and beta (ERα and ERβ). Recent evidence has indicated that a rapid, nongenomic, nonclassical estrogen signaling pathway exists via the G protein–coupled estrogen receptor (GPER), which is expressed in many biological systems, with roles in the cardiovascular system. The objective of the present study was to investigate the effect of 17α-ethinylestradiol (EE2) on the heart rate of embryonic Japanese medaka (Oryzias latipes). A significant decrease (bradycardia) in embryonic heart rate was observed at all treatment concentrations (0.1, 1, 10, 100, and 1000 ng/L EE2) at 144, 168, and 192 h postfertilization (hpf; p ≤ 0.05), whereas 120 and 216 hpf embryos experienced a significant decrease from the control at 10, 100, and 1000 ng/L EE2 and 0.1, 100, and 1000 ng/L EE2, respectively (p ≤ 0.05). In addition, using select estrogen receptor modulators, it was demonstrated that estrogen-induced bradycardia appears to be linked to GPER and not ERα and ERβ. The present study highlights GPER as a novel and alternative mode of action for EE2 toxicity at environmentally relevant concentrations. Environ Toxicol Chem 2020;39:904–912. © 2020 SETAC
SWI/SNF chromatin remodeling complex and glucose metabolism are deregulated in advanced bladder cancer
Authors: Stachowiak Malgorzata , Szymanski Michal , Ornoch Anna , Jancewicz Iga , Rusetska Natalia , Chrzan Alicja , Demkow Tomasz , Siedlecki Janusz A. , Sarnowski Tomasz J. , Sarnowska Elzbieta 
Published: February 2020

AbstractBladder cancer (BC) is a frequently diagnosed malignancy affecting predominantly adult and elderly populations. It is expected that due to the longer life time, BC will become even more frequent in the future; thus in consequence, it will represent serious health problem of older society part. The treatment of advanced BC is mostly ineffective due to its very aggressive behavior. So far, no effective targeted therapy is used for BC treatment. Here, we found that BC is characterized by lower protein levels of BRM, INI1, and BAF155 main subunits of SWI/SNF chromatin remodeling complex (CRC) which is involved in global control of gene expression and influences various important cellular processes like: cell cycle control, apoptosis, DNA repair, etc. Moreover, the expression of SMARCA2, a BRM encoding gene, strongly correlated with BC metastasis and expression of such metabolic genes as PKM2 and PRKAA1. Furthermore, the analysis of T24 and 5637 commonly used BC cell lines revealed different expression levels of metabolic genes including FBP1 gene encoding Frutose‐1,6‐Bisphosphatase, an enzyme controlling glycolysis flux and gluconeogenesis. The tested BC cell lines exhibited various molecular and metabolic alterations as well as differential glucose uptake, growth rate, and migration potential. We have shown that BRM subunit is involved in the transcriptional control of genes encoding metabolic enzymes. Moreover, we found that the FBP1 expression level and the SWI/SNF CRCs may serve as markers of molecular subtypes of BC. Collectively, this study may provide a new knowledge about the molecular and metabolic BC subtypes which likely will be of high importance for the clinic in the future.
Impact of a mental health trainee interdisciplinary program on a veteran population
Authors: Karst Allison , Colvard Michelle , Bean Jennifer , Patel Erin , Pate Rebecca , Lister Jonathan 
Published: February 2020

AbstractBackgroundLess than half of adults with mental illness receive mental health services. Several barriers exist to accessing care including stigma and shortage of mental health clinicians. Due to the increasing demand for mental health services, the American health care system has begun moving toward an interprofessional approach.ObjectiveThe primary objective was to evaluate the impact of a trainee‐led interprofessional mental health team on treatment outcomes.MethodsThis single‐center, retrospective, observational analysis was conducted at a Veterans Affairs medical center from August 2013 through April 2019. Eligible patients were identified using the clinic roster and manual review was utilized to exclude patients based on defined criteria. Patient demographics, referral source, initial appointment date, enrollment status, psychiatric diagnoses, and mental health assessment measures were collected. The primary outcome was change in Patient Health Questionnaire (PHQ‐9), General Anxiety Disorder‐7 (GAD‐7), and Post‐Traumatic Stress Disorder (PTSD) Checklist for Diagnostic and Statistical Manual of Mental Disorders Fifth Edition (PCL‐5) total score from clinic intake to discharge.ResultsThere was a statistically significant reduction in PHQ‐9 (−6.00; −38.54%; P < .001), GAD‐7 (−4.35; −33.38%; P < .001), and PCL‐5 (−19.33; −34.29%; P < .001) total scores. Additionally, there was a statistically significant change in PTSD remission rate per PCL‐5 (+33.3%; P = .001). There was a numerical reduction in mental health‐related emergency department visits (−3; P = .495), psychiatric hospitalizations (−7; P = .182), and psychiatric hospital days (−53; P = .229).ConclusionsA trainee‐led interdisciplinary mental health team positively impacted depression, anxiety, and PTSD related outcomes in a Veteran population. Results support further study and incorporation of the interdisciplinary care model into outpatient mental health settings.