bluuebunny/crossref_metadata_2025_split huggingface dataset:
Response of kutuklin hemaglutinin protein adhesion in koi fish (Cyprinus carpio) infected by Myxobolus sp.
Authors: Yanuhar U , Junirahma N S , Caesar N R , Musa M , Mahasri G 
Published: February 2020

Abstract
Koi fish (Cyprinus carpio) cultivation is quite easy to maintain if it can look carefully at fish health well. The Difficulty in maintenance due to parasitic attacks is Myxobolus sp., and this parasite is capable of causing death in fish. Treatment of parasites attached to koi fish can be done by giving chemicals such as Pyrethrum. This research aims to determine the reaction of kutuklin hemagglutination adhesin to koi fish infected by Myxobolus sp. The method used is an explorative and descriptive by conducting an In-Vivo test on koi fish. The treatment is, healthy fish without drug as a control, healthy fish with a drug dose of 1 µl/g feed, and fish were infected with a drug dose of 1 µl/g. Observation is done by taking fish blood plasma in each treatment. The results showed that kutuklin was able to contribute to treating Myxobolus sp. However, it cannot restore such a healthy state. This is indicated by high (up to 1/1024 dilution) in the hemagglutination (HA) test of koi fish blood plasma. So, that kutuklin can be used as an immune suppressor (immunosuppressor).
Effect of different filter media use on aquaponics system on ammonium (NH4
+), nitrite (NO2) and nitrate (NO3) concentrations of catfish (Clarias sp.) aquaculture
Authors: Lukmantoro T A , Prayogo  , Rahardja B S 
Published: February 2020

Abstract
Catfish cultivation has been carried out in Indonesia. One such development is the maintenance of catfish using an aquaponic system. Aquaponics aims to improve the water quality of fish farming with the help of plants. But plants cannot directly utilize fish farming waste directly, it is necessary to have a nitrification process first. Nitrification is the reformation of inorganic compounds into organic compounds with the help of bacteria. Nitrifying bacteria are colonized and non-motile bacteria, so a substrate is needed as a place to attach to the bacteria. The substrate that can be used is bioball and bioring. This study aims to determine whether the effect and filter media are effective in catfish farming with an aquaponic system. This study using the RAL method consisting of 4 treatments and 5 replications, namely P0 = control, P1 = bioball, P2 = bioring and P3 = bioball and bioring combinations. The results obtained that P3 (bioball and bioring combination) is the most effective treatment in reducing the nitrogenous waste catfish aquaculture using an aquaponics system, the results is 0,000 mg / L ammonium, nitrite 0.001 mg / L and nitrate 13.0 mg / L at the end of the study.
Different addition of molasses on feed conversion ratio and water quality in catfish (Clarias sp.) rearing with biofloc-aquaponic system
Authors: Rahmatullah H D , Prayogo  , Rahardja B S 
Published: February 2020

Abstract
Biofloc system can overcome the problem on intensive system aquaculture by convert nitrogenous waste into microbial mass that can consumed by fish. Moreover, aquaponics systems that culture fish and plants also able to increase crop yields and improve water quality at the same time. Combination from those two system could create synergism to maximize the effects from both system. This study aims to determine the effect of molasses addition on feed conversion ratio (FCR) and water quality (TAN, nitrite and nitrate) of catfish rearing by biofloc-aquaponic system. This experimental study was carried out for 30 days using a Completely Randomized Design (CRD) consisting of four treatments with five replications. Those four treatments are based on the difference in the amount of molasses added. Data were analyzed by analysis of variance (ANOVA) followed by Duncan multiple range test (DMRT). The results of this study indicate that the different addition of molasses is able to bring a positive effect on FCR and water quality in the catfish rearing using biofloc-aquaponic system with the lowest FCR obtained by P3 (1.33) and the lowest nitrogenous waste obtained by P2 but the FCR were not significantly different with P3.
Composition analysis of organic and inorganic waste and the impacts of coastal city in Palu-Central Sulawesi
Authors: Walalangi J Y , Lelono T D , Suryanto A M , Damar A , Effendi H , Susilo E 
Published: February 2020

Abstract
Organic and anorganic waste in Palu city has exceeded the capacity of the service and the existing waste management facilities so that waste piling up in landfills while (TPS), and locations of residential areas around the watershed that eventually the waste to the sea. Comprehensive research is needed to formulate the management of such waste. It is given because the higher the level of human activity residing in the city of Palu, has brought the issue of marine pollution in the Palu bay, namely through the waste dump along the Watershed (DAS) Palu which empties into the sea. It can be seen through the rubbish heaps of the sea at low tide, be it in the estuaries and bays along the coast of Palu. So far, the management of coastal waste less attention than the waste in the city. It is time for the attention given to the coastal environment is given the function of these coast systemic interplay of other ecosystems. Integrated management and the sustainable coastal environment will preserve it so that its function will be maintained properly and as intended. The success of this integrated waste management depends on community participation, as the main producer of waste.
Comparison of water quality and its influences on phytoplankton abundance based on water characteristics in coastal of Banyuwangi Regency, Jawa Timur, Indonesia
Authors: Setyaningrum E W , Masithah E D , Yuniartik M , Nugrahani M P , Dewi A T K 
Published: February 2020

Abstract
Coastal waters of Banyuwangi Regency, East Java, Indonesia obtains water resources from residential areas, the fishing industry, the shipping industry in the surrounding area. This study aims to determine the influences of water quality on phytoplankton abundance, seen from differences characteristics of coastal waters of Banyuwangi. The research method is descriptive. Data collection technique is observation, with the required data including water quality parameters (Sn, PO4, Total Organic Matter, NO3, Cu Copper, DO, Water Temperature, Pb, Salinity, Alkalinity, pH, and NH4) and phytoplankton. The analysis used plankton abundance analysis calculated using the Lachlan modification formula (1982), and multiple regression analysis. The analysis result showed that the type of phytoplankton found was green algae Chlorella type and blue-green algae Oscillatoria type. The abundance of phytoplankton in the coastal area of Banyuwangi is around 12.5 - 513.75 ind/L, where it is included in the level of eutrophic phytoplankton abundance, which waters in a high fertility. There is an influence between water quality on the abundance of phytoplankton on the coast of Wongsorejo, Banyuwangi and Blimbingsari Districts. Whereas in Lampon (Pesanggaran subdistrict), water quality does not affect the abundance of phytoplankton.
Different concentration influence of Moringa oleifera leaf aqueous extract immersion against Argulus japonicus egg damage
Authors: Idris F , Kismiyati  , Mahasri G 
Published: February 2020

AbstractArgulus japonicusis an ectoparasite that invades freshwater fish, causing ulceration and bleeding, besides opening the secondary infections access for bacteria, fungi, viruses and triggering fish death.A. japonicusefficient control can be done by cutting the ectoparasite life cycle, specifically the egg stage, using natural insecticide. One of the natural insecticides isM. oleiferaleaves as it has phytochemical contents, i.e., tannins, flavonoids, alkaloids, and toxic saponins for eggsA. japonicus. The study aimed to analyze the immersion effect ofM, oleiferaleaf aqueous extract under different concentrations againstA. japonicusegg damage and determine the optimal concentration ofM. oleiferaleaf aqueous extract that significantly damagedA. japonicusegg until 100%. This study used four aqueous extract treatment, namely 0% (A), 4% (B), 6% (C), and 8% (D) with five times repetitions. The results showed that each treatment had significant influence againstA. japonicusegg damage (P < 0.05). The lowestA. japonicusegg damage was found at A treatment, while the highest was at D treatment.M. oleiferaleaf aqueous extract can be utilized as a natural insecticide to disrupt the egg phase ofA. japonicuswith 8% concentration in aqueous extract.
The effect of distance of floating karamba on placement of phytoplankton abundance in coastal waters of Sathean Village, Langgur - Tual Regency
Authors: Isroni W , Bahri A S , Amin A A 
Published: February 2020

Abstract
The condition of water will also affect the distribution pattern or distribution of phytoplankton both horizontally and vertically, so that it will affect the abundance of phytoplankton which in turn affects the primary productivity value. The waters of Sathean Village are some floating net cages (KJA) owned by both the community and the company. The existence of economic activities in the waters of Sathean Village, it results in disruption of the balance of the aquatic environment which ultimately affects the growth of phytoplankton in these waters. The study was conducted using an experimental method with a factorial completely randomized design. The first research factor was the distance of KJA placement with 3 sub-factors, namely 26 m distance; 38 m; and 52 m. The second research factor is the depth of sampling consisting of 4 depths namely 0 m; 2 m; 4 m; and 6 m. The test is done 3 times. Station B, a distance of 38 m from the beach 38 from the beach with a sampling depth of 0m, 2m, 4m and 6m, and 3 replications. The main parameters observed were phytoplankton abundance, diversity index, uniformity index, and dominance index. Meanwhile, the supporting factor is water quality.
The effect of addition of fish bone meal on the concentration of nitrogen (N), phosphorus (P), and potassium (K) in seaweed liquid organic fertilizer of Gracilaria sp.
Authors: Bhaskoro P T , Tjahjaningsih W , Mubarak A S 
Published: February 2020

Abstract

Gracilaria sp. contains macro and micronutrients needed by plants, such as phosphorus, potassium, calcium, magnesium, manganese, zinc, and boron. Seaweed-based liquid organic fertilizer currently on the market has low phosphorus and potassium content. One ingredient that can increase phosphorus and potassium content is fishbone. This study aimed to determine the effect of fishbone meal addition to Gracilaria sp. liquid organic fertilizer on the concentration of nitrogen, phosphorus, and potassium. The method used in this study was the experimental method with four treatments and five replications on each treatment. These treatments were P0 (0% fish bone meal), P1 (25% fish bone meal), P2 (50% fish bone meal), and P3 (75% fish bone meal). The main parameters in this study were the concentration level of nitrogen, phosphorus, and potassium. The addition of fishbone meal increased nitrogen, phosphorus, and potassium concentration. The highest nitrogen, phosphorus, and potassium contents were found in the treatment with the addition of 75% fish bone meal, producing 0.69% nitrogen, 0.42% phosphorus, and 0.43% potassium content.
Isolation and identification of bacteria in gastrointestinal of eel (Anguilla bicolor) that has potential as probiotic
Authors: Lestari P , Suprapto D , Mahasri G 
Published: February 2020

Abstract
Antibiotic usage in aquaculture serves for the prevention and treatment of diseases but causes an accumulation of residues, and the development of resistant bacterial strains that demands the use of probiotics is increasing. The quality of fish not only seen on the weight of fish but also of bacteria associated with the digestive tract. Some types of bacteria found in the digestive tract of animals have an essential role in order to improve the utilization of feed, fish health, and improvement of environmental quality and microorganisms. The research aims to determine the type of bacteria that can be isolated and characterized from the gastrointestinal tract Anguilla bicolor and potential as a candidate probiotic bacteria. This study used data analysis with descriptively. The gastrointestinal tract is the intestine isolation and characterization of bacterial strains obtained. The observations made include morphological characters, hydrolysis tests (fat, starch, protein), and identification of each isolate. From the digestive tract, Anguilla bicolor found seven bacterial isolates based on the identification and competent to hydrolyze protein, fat, and starch consists of 3 species thought to be used as probiotics Anguilla bicolor are Bacillus subtilis, Bifidobacterium and Lactobacillus plantarum.
Effect of fucoidan concentration from Sargassum sp. on skin lotion antioxidant activities
Authors: Putra R C D , Alamsjah M A , Sulmartiwi L 
Published: February 2020

Abstract
Fucoidan is a polysaccharide with complex sulfate groups that are found in the cell walls of brown seaweed, one is Sargassum sp. This study aimed to determine the effect of addition fucoidan from Sargassum sp. on the antioxidant activity of skin lotion and to determine antioxidant activity of skin lotion with the addition of antioxidants after storage. The method used was experimental with completely randomized design (RAL) with four treatments and five replications. The treatments used are: P1 (fucoidan 1 X IC50), P2 (fucoidan 2 X IC50), P3 (fucoidan 3X IC50) and Control (Skin lotion with green tea extract commercial).The antioxidant activity testis done with DPPH method. The results showed that the IC50 value of fucoidan extract were 1407.667 ppm. In the result of adding fucoidan on skin lotion prode result IC50 values P1, P2, P3 and Control in a row was 1709, 816, 645 and 314 ppm. While the IC50 value of skin lotion 30th day in a row was 1723, 825, 699 and 323 ppm. Based on these results, the skin lotion with fucoidan has a weak antioxidant activity because it had IC50 values above 200 ppm, while storage for 30 days can increase the value of IC50.
Correlation between water quality and prevalence on Koi (Cyprinus carpio) which infested by Argulus in Mungkid Subdistrict and Muntilan Subdistrict, Magelang Regency, Central Java
Authors: Yunikasari R D , Kismiyati  , Mahasri G 
Published: February 2020

AbstractKoi (Cyprinus carpio) has an interesting added value which one of the most famous freshwater ornamental fish. There are a lot of factors which have to be faced when we were trying to cultivate or culture Koi; one of those factors is the disease. Ectoparasite is one factor that can be the decline on koi selling because the ectoparasite can reduce the selling point caused by the effect on koi. One of ectoparasite known as attack koi isArgulus. The purpose of this research to determine relations between water quality and the prevalence ofArguluson Koi (Cyprinus carpio). The research was conducted at Magelang, Central Java (on Mungkid’s and Muntilan’s region). Measurement of water quality was temperature, pH, DO, and ammonia. This research use survey method and take the sample with random sampling. The result of this research shown that the prevalence of Koi that infested byArgulusin Mungkid subdistrict are 30.77 %, and in the Muntilan sub-district are 55.17%. There was a positive correlation between water quality and the prevalence ofArgulusespecially at the temperature, which has a significant correlation. Higher temperatures will also increase theArgulusprevalence.
Effect of eggs immersion in tanin solution against embryonic development of common carp fish (Cyprinus carpio L.)
Authors: Fauziah N , Masithah E D , Sulmartiwi L 
Published: February 2020

Abstract
Common carp (Cyprinus carpio L.) is a freshwater fish that has great potential to be developed, because it have high nutritional content. The eggs carp that is cultivated naturally have a high mortality rate, almost 80. Tannins have the ability to bind and precipitate proteins the caused by the presence of a number of functional groups that will bond strongly with protein molecules interacted which in turn will generate substantial crosslinking and tannin-protein complex that. This study was conducted to determine the effect of soaking the eggs in a solution of tannin on the embryonic development of carp. Method used in this study using quantitative descriptive method. The results showed that the soaking time tannin solution can affect to the rate of embryonic development of common carp at each phase. In the treatment of 120 and 180 seconds embryos develop faster in the early phase and the final phase is slower than the control. This suggests that the tannin solution can Accelerate the development of the embryo in the early phase and the discovery of abnormal larvae hatch in the treatment of 180 seconds.
The effect of giving fermented rice bran suspension on fecundity and production of moina macrocopa offspring per parent
Authors: Damayanti K Y , Mubarak A S , Sari L A 
Published: February 2020

Abstract
The objectives of this research were: 1) determine the effect of fermented rice bran suspension concentration on the fecundity and production of M. macrocopa offspring per parent, 2) determine the optimal concentration of fermented rice bran suspension on the fecundity and production of M. macrocopa offspring per parent. This research used an experimental method with a completely randomized design consisting of six treatments of suspension of rice bran without fermentation (P0), 19.54 mg / L fermented rice bran suspension (P1), 21.98 mg / L fermented rice bran suspension (P2), 24.42 mg / L fermented rice bran suspension (P3), 26.68 mg / L fermented rice bran suspension (P4) and 29.30 mg / L fermented rice bran suspension (P5) in four replications for each. The results of the culture with a different concentration of rice bran suspension showed a significant difference (P <0.05) on the fecundity and production of M. macrocopa offspring per parent. The highest fecundity and production of M. macrocopa offspring per parent occurred in M. macrocopa culture with a concentration of fermented rice bran suspension of 29.30 mg / L (P5) with the fecundity 21-25 grains/parent and production of offspring 20-24 ind/parent.
Correlation between Osteoprotegerin Serum Levels and Arterial Stiffness Assessed by Cardio-ankle Vascular Index (CAVI) in Hypertensive Patients
Authors: Ariendanie A , Putranto J N E , Ranuh I G N I R 
Published: February 2020

Abstract
The cardio-ankle vascular index (CAVI) is a newly developed clinical measurement for evaluating arterial stiffness from the origin of the aorta to the ankle. Osteoprotegerin (OPG) is widely known as a vascular calcification mediator, which have a role to affect atherogenesis. Thirty subjects participated in this research and each subject underwent a CAVI examination to assess arterial stiffness and their blood samples were collected for OPG measurement. This study is analyzed with Pearson Correlation Test. There is a positive, strong and significant correlation between osteoprotegerin serum level and arterial stiffness using Cardio-Ankle Vascular Index (CAVI) in hypertensive patients. (r = 0.730 and p <0,0001). There was a positive, strong and significant correlation between osteoprotegerin serum level and arterial stiffness using Cardio-Ankle Vascular Index (CAVI) in hypertensive patients.
Hypoxic Preconditioning Effect on the Expression of Intracellular Heat Shock Protein (HSP) 27, HSP 70 and HSP 90 on Cultured Adipocyte-Derived Mesenchymal Stem Cells (AMSCs)
Authors: Ghaznawie A F , Suryawan I G R , Andrianto A , Romdiyana A 
Published: February 2020

Abstract
New hope in the treatment of myocardial infarction has emerged by transplanting multipotent stem cells into the affected myocardium. Hypoxic preconditioning (HPC) is expected to increase the survival cell potency by inducing upregulation of Heat Shock Protein (HSP) in Adipocyte-derived Mesenchymal Stem Cells (AMSCs). This study aimed to investigate the roles of HPC in AMSCs to observe the in vitro expression of HSP27 as an ATP independent chaperone, and HSP70, HSP90 as an ATP dependent chaperone. This study is a true experimental randomized post-test design study. AMSCs were isolated from adipose tissue and cultured until there were four passages. The samples were then divided into two groups, HPC (O2 1%) and normoxia (O2 21%). Immunocytofluorescence techniques were used to evaluate the expression of HSP27, HSP70, and HSP90. Data were analyzed using a two paired t-test and Mann Whitney U test. There was a significant increase of HSP27, HSP70, and HSP90 expression in HPC compared to the normoxia group (272,00±92,225 vs 86,19±26,362 p<0.0001; 130,88±45,416 vs 120,75±97,113 p<0.05; 165,75±58,930 vs 96,81±22,578). Hypoxic preconditioning significantly increased the expression of HSP27, HSP70, and HSP90 on cultured AMSCs.
Hypoxic Preconditioning Effects of Bone Marrow-derived Culture Mesenchymal Stem Cells on CD31+ Expression, Vascular Endothelial Growth Factors-a (VEGF-A) and Stromal-derived Sactors-1 Alpha (SDF-1α)
Authors: Muzakkir A F , Suryawan I G R , Yusrizal T 
Published: February 2020

Abstract
Increasing the ability of survival stem cells can be determined by assessing the state of hypoxic preconditioning (HPC) in stimulated cells. The assessment is carried out through bone marrow-derived stem (BMSCs) cultures obtained from the femoral bone marrow aspiration procedure (Wismar Rat). This study aimed to prove the hypothesis that there is CD31+ expression on HPC cultures; also, the concentration of VEGF-A and SDF-1α were higher in HPC than control. This research is designed as a laboratory experiment for a period of three months. Male Wistar rat (n = 16) was divided into 2 groups: P0 controls (21% [O2]) and P1 treatments (1% [O2]). Each sample was run into eight repetitions and observed for 48 hours. The stages in this study include isolation and culture of BMSCs, identification of phenotypic BMSCs based on the expression of cell-surface markers (CD105+, CD34−) and hypoxic exposure. After that, it was observed that the potential of angiogenesis in samples was based on CD31+ expression, the paracrine activity of vascular endothelial growth factor-A (VEGF-A) and stromal-derived factor-1 Alpha (SDF-1α). The results showed that there is no CD31+ expression on P1. A of VEGF-A and SDF-1⌑ were higher in P1 than P0.
Neglected Cases of Hypertension in Rural Indonesia: A Cross-Sectional Study of Prevalence and Risk Factors on Adult Population
Authors: Mulia E P B , Prajitno S 
Published: February 2020

Abstract
Hypertension (HTN) is a significant health problem because of the high risk of cardiovascular morbidity and mortality. In rural areas, awareness and health facilities are limited, and report on the prevalence and related risk factors of hypertension is scarce. This study aimed to investigate the prevalence and associated factors of hypertension in the rural population in Indonesia. A community-based cross-sectional study was conducted to adult subjects from a rural population in Jombang, East Java, Indonesia. Data were collected by interviews to obtain socio-demographic characteristic and risk factors. Blood pressure measurements were performed using a mercury sphygmomanometer. We enrolled a total of 121 adult subjects. The prevalence of hypertension was 38.8%. Among hypertensive patients, 83% of them do not seek nor receive any treatment. The prevalent risk factors of hypertension in this study were age more than 40 years old (OR=4.1; 95%CI:1.8-8.9), abdominal obesity (OR=2.7; 95%CI: 1.2-6.01), and smoking (OR=3.2; 95%CI: 1.4-7.1). From our study, the prevalence of hypertension was high in rural adult population, and most of them did not receive treatments. Smoking and abdominal obesity are two risk factors that can be modified as one of the prevention strategies. These phenomena requires the attention of health workers.