bluuebunny/crossref_metadata_2025_split huggingface dataset:
In vivo kinetics of the genotoxic and cytotoxic activities of cladribine and clofarabine
Authors: Quezada‐Vidal Jesús , Ortíz‐Muñiz Rocío , Cervantes‐Ríos Elsa , Cruz‐Vallejo Virginia , Morales‐Ramírez Pedro 
Published: June 2020

AbstractThe aim of the present in vivo study was to determine the kinetics of the genotoxic and cytotoxic activities of cladribine and clofarabine in mouse normoblasts using flow cytometry. Mice in groups of five were treated with cladribine or clofarabine. Blood samples were obtained from the mouse tails before treatment and every 8 hr posttreatment for 72 hr. These samples were cytometrically scored for micronucleated reticulocytes (RETs), and the percentage of RETs was determined. The results showed that clofarabine and cladribine have early cytotoxic effects that are related to the genotoxic effects reported in previous studies; the drugs have both complex long‐lasting genotoxic and cytotoxic kinetic activity, with similar profiles that suggest a relationship between the genotoxic and cytotoxic parameters. The initial genotoxkinetics timing of clofarabine is equivalent to those of difluorodeoxycytidine, likely because both agents inhibit DNA polymerase. Clofarabine shows a higher genotoxic and cytotoxic efficiency than cladribine, in agreement with previous results.
Design of a novel hybrid control strategy for multi‐inverter parallel system for resonance suppression
Authors: Zheng Feng , Lin Xiangqun , Zhang Yachao , Deng Changhong 
Published: June 2020

AbstractComparing with the LCL‐type single‐inverter parallel system, the multi‐inverter parallel system has more complex resonance characteristics which are generated by three type resonance sources (reference current changing in the inverter control system, reciprocal effect among multi‐inverters and grid background harmonic) and cause great negative effects on power system. In order to suppress the resonance problem of LCL‐type multi‐inverters parallel system, a novel control strategy is proposed in this paper based on model current predictive control (MCPC) and two‐degree‐of‐freedom control. Through MCPC, this strategy can reduce the number of current inner‐loop PI of control layer and remove PWM module. Then, a potential robustness can be obtained. From the outer loop of control layer perspective, because MCPC's value function consists of inverter's output current and reference current of control layer inner loop, they can be approximately regarded as equal. And then through constructing the inverse model of control object, the two‐degree‐of‐freedom control can be introduced to realized transfer function unitarization of control layer's outer loop, so that the order difference between numerator's highest order term and denominator's highest order term of inverter resonance transfer function can be reduced. Accordingly, it can be found that with the three type resonance sources, there is no peak value in their resonance transfer functions and their gains all present attenuation effects. Therefore, the resonance problems of multi‐inverter parallel system can be eliminated. Through the theoretical derivation and simulation results, it can be proved that the proposed method can not only enhance the transient performance of multi‐inverter parallel system, but also its resonance problems.
Machine‐learning‐based surface tension model for multiphase flow simulation using particle method
Authors: Liu Xiaoxing , Morita Koji , Zhang Shuai 
Published: June 2020

SummaryParticle methods have shown their potential for simulating multiphase flows due to the convenience in capturing interfaces. However, when it comes to estimate the surface tension, calculation of the curvature of the interface remains challenging. Traditional methods are based on derivative models to estimate the curvature analytically from the particle number density or color function that marks different phases. It is difficult to estimate the curvature accurately in traditional derivative models. In this study, background cells are built up and are used to predict the curvature through machine learning. By training on a data set generated using circles of varying sizes, a relation function is found to predict the curvature from the particle distribution near the interface. Together with the enhanced schemes developed in our previous study, multiphase flows with surface tension are studied within the framework of the moving particle semi‐implicit method.
Group analysis of distance matrices
Authors: Wang Jinjuan , Li Jialu , Xiong Wenjun , Li Qizhai 
Published: June 2020

AbstractDistance‐based regression model has become a powerful approach to identifying phenotypic associations in many fields. It is found to be particularly useful for high‐dimensional biological and genetic data with proper distance or similarity measures being available. The pseudo F statistic used in this model accumulates information and is effective when the signals, that is the variations represented by the eigenvalues of the similarity matrix, scatter evenly along the eigenvectors of the similarity matrix. However, it might lose power for the uneven signals. To deal with this issue, we propose a group analysis on the variations of signals along the eigenvalues of the similarity matrix and take the maximum among them. The new procedure can automatically choose an optimal grouping point on some given thresholds and thus can improve the power evidence. Extensive computer simulations and applications to a prostate cancer data and an aging human brain data illustrate the effectiveness of the proposed method.
Safety parameters of ferromagnetic device during thyroid surgery: Porcine model using continuous neuromonitoring
Authors: Huang Tzu‐Yen , Lin Yi‐Chu , Tseng Hsin‐Yi , Dionigi Gianlorenzo , Kim Hoon Yub , Chai Young Jun , Lu I‐Cheng , Chang Pi‐Ying , Chiang Feng‐Yu , Wu Che‐Wei 
Published: June 2020

AbstractBackgroundThe ferromagnetic (FM) device is a new surgical energy modality. This study investigated dynamic recurrent laryngeal nerve (RLN) electromyographic (EMG) data to define safety parameters for using FM devices in thyroidectomy.MethodsReal‐time EMG tracings obtained under continuous neuromonitoring were recorded from 24 RLNs (12 piglets). In the activation study, FM devices were activated at varying distances from the RLN. In the cooling study, FM devices were cooled for varying time intervals, or muscle touch maneuver was performed, before contact with the RLN.ResultsDuring the FMwand/FMsealer activation, no adverse EMG events occurred at distances of 2 mm or longer. The cooling study revealed no adverse EMG events after 1‐second (FMwand) or 3‐second (FMsealer) intervals or after muscle touch maneuver.ConclusionsAn FM device should be activated at a distance of 2 mm from the RLN and should be adequately cooled before further contact with the RLN. Surgeons can avoid RLN injury by observing standard procedures for using FM devices.
An Endoplasmic Reticulum Stress–MicroRNA‐26a Feedback Circuit in NAFLD
Authors: Xu Haixia , Tian Yan , Tang Dongmei , Zou Sailan , Liu Geng , Song Jiulin , Zhang Guixiang , Du Xiao , Huang Wei , He Bin , Lin Weiqiang , Jin Liang , Huang Wendong , Yang Jiayin , Fu Xianghui 
Published: June 2020

Background and Aims
Endoplasmic reticulum (ER) stress is an adaptive response to excessive ER demand and contributes to the development of numerous diseases, including nonalcoholic fatty liver disease (NAFLD), which is hallmarked by the accumulation of lipid within hepatocytes. However, the underlying mechanisms remain elusive. MicroRNAs (miRNAs) play an indispensable role in various stress responses, but their implications in ER stress have not yet been systemically investigated. In this study, we identify a negative feedback loop consisting of hepatic ER stress and miR‐26a in NAFLD pathogenesis.

Approach and Results
Combining miRNA dot blot array and quantitative PCR, we find that miR‐26a is specifically induced by ER stress in liver cells. This induction of miR‐26a is critical for cells to cope with ER stress. In human hepatoma cells and murine primary hepatocytes, overexpression of miR‐26a markedly alleviates chemical‐induced ER stress, as well as palmitate‐triggered ER stress and lipid accumulation. Conversely, deficiency of miR‐26a exhibits opposite effects. Mechanistically, miR‐26a directly targets the eukaryotic initiation factor 2α, a core ER stress effector controlling cellular translation. Intriguingly, miR‐26a is reduced in the livers of patients with NAFLD. Hepatocyte‐specific restoration of miR‐26a in mice significantly mitigates high‐fat diet–induced ER stress and hepatic steatosis. In contrast, deficiency of miR‐26a in mice exacerbates high‐fat diet–induced ER stress, lipid accumulation, inflammation and hepatic steatosis.

Conclusions
Our findings suggest ER stress–induced miR‐26a up‐regulation as a regulator for hepatic ER stress resolution, and highlight the ER stress/miR‐26a/eukaryotic initiation factor 2α cascade as a promising therapeutic strategy for NAFLD.
Exploratory analysis of intensified conditioning as first line treatment for patients with high risk multiple myeloma
Authors: Bommier Côme , Talbot Alexis , Harel Stéphanie , Cuccuini Wendy , Gérard Laurence , Arnulf Bertrand 
Published: June 2020

AbstractMultiple myeloma has extremely heterogeneous outcomes. Among prognostic factors, t(4;14) and del(17p) are rare oncogenic events associated with very poor prognosis. In an exploratory case‐control study, we compared the combination of Busulfan‐Melphalan or TBI‐Melphalan with high dose Melphalan as a conditioning regimen in a series of 48 patients with del(17p) or t(4;14). These regimens were preceded by a Bortezomib‐containing induction. Progression‐free survival (PFS) was the primary endpoint whereas overall survival (OS) and complete response (CR) rate were the secondary endpoints. Twenty consecutive cases of high‐risk myeloma received a reinforced conditioning regimen of Busulfan 0.8 mg/kg x4/j IV from day‐6 to day‐3 pre‐ graft (BuMel) or total body irradiation (TBI) 12 Gy (TbiMel), having received Melphalan 140 mg/m2 at day‐2 pre‐graft. These cases were matched to 28 controls treated with Melphalan 200 mg/m2 at day‐2 (Mel200). After intensification ± consolidation, with a median follow‐up of 6.3 years, the CR rate was higher in the BuMel/TbiMel group (65% vs 50%, P = .006). No differences were observed between both groups in terms of PFS and OS (P = .96). PFS in patients with a del(17p) mutation tended to be superior in the BuMel/TbiMel group. Our exploratory study shows that reinforcing the intensification regimen with Busulfan or TBI does not seem to improve the prognosis associated to t(4;14) and del(17p) abnormalities.
Clinicopathological analysis of immunohistochemical expression of CD47 and SIRPα in adult T‐cell leukemia/lymphoma
Authors: Yanagida Eriko , Miyoshi Hiroaki , Takeuchi Mai , Yoshida Noriaki , Nakashima Kazutaka , Yamada Kyohei , Umeno Takeshi , Shimasaki Yasumasa , Furuta Takuya , Seto Masao , Ohshima Koichi 
Published: June 2020

AbstractThe interaction of CD47 and signal‐regulatory protein alpha (SIRPα) induces “don't eat me signal”, leading suppression of phagocytosis. This signal can affect the clinical course of malignant disease. Although CD47 and SIRPα expression are associated with clinicopathological features in several neoplasms, the investigation for adult T‐cell leukemia/lymphoma (ATLL) has not been well‐documented. This study aimed to declare the association between CD47 and SIRPα expression and clinicopathological features in ATLL. We performed immunostaining on 73 biopsy samples and found that CD47 is primarily expressed in tumor cells, while SIRPα is expressed in non‐neoplastic stromal cells. CD47 positive cases showed significantly higher FoxP3 (P = .0232) and lower CCR4 (P = .0214). SIRPα positive cases presented significantly better overall survival than SIRPα negative cases (P = .0132). SIRPα positive cases showed significantly HLA class I (P = .0062), HLA class II (P = .0133), microenvironment PD‐L1 (miPD‐L1) (P = .0032), and FoxP3 (P = .0229) positivity. In univariate analysis, SIRPα expression was significantly related to prognosis (Hazard ratio [HR] 0.470; 95% confidence interval [CI] 0.253‐0.870; P = .0167], although multivariate analysis did not show SIPRα as an independent prognostic factor. The expression of SIRPα on stromal cells reflects activated immune surveillance mechanism in tumor microenvironment and induce good prognosis in ATLL. More detailed studies for gene expression or genomic abnormalities will disclose clinical and biological significance of the CD47 and SIRPα in ATLL.
Impulse noise reduction using hybrid neuro‐fuzzy filter with improved firefly algorithm from X‐ray bio‐images
Authors: Pugalenthi R. , Oliver A. Sheryl , Anuradha M. 
Published: June 2020

AbstractNoise filtering performance in medical images is improved using a neuro‐fuzy network developed with the combination of a post processor and two neuro‐fuzzy (NF) filters. By the fact, the Sugeno‐type is found to be less accurate during impulse noise reduction process. In this paper, we propose an improved firefly algorithm based hybrid neuro‐fuzzy filter in both the NF filters to improve noise reduction performance. The proposed noise reduction system combines the advantages of the neural, fuzzy and firefly algorithms. In addition, an improved version of firefly algorithm called searching diversity based particle swarm firefly algorithm is used to reduce the local trapping problem as well as to determine the optimal shape of membership function in fuzzy system. Experimental results show that the proposed filter has proved its effectiveness on reducing the impulse noise in medical images against different impulse noise density levels.
Behavior of Gammarus aequicauda (Crustacea, Amphipoda) during predation on Artemia (Crustacea, Anostraca): New experimental results
Authors: Shadrin Nickolai , Yakovenko Vladimir , Anufriieva Elena 
Published: June 2020

AbstractGammarus aequicauda and Artemia spp. are abundant crustacean species in Crimean hypersaline lakes. G. aequicauda preys on Artemia but there was no quantitative data on this before the current study. Predation of G. aequicauda on adult Artemia was studied in experiments with two different approaches evaluating (a) the time balance of the feeding process and (b) the grazing intensity. The threshold prey concentration, when consumption began to increase with increasing concentration, was approximately 15 ind./L in 200‐ml vessels and about 5 ind./L in 500‐ml vessels. When the Artemia abundance reached 20–25 ind./L, there was no further influence on the gammarid consumption rate. There was a significant negative correlation between the consumption rate of gammarids and Artemia abundance. According to study results, an individual G. aequicauda may eat up to 20–24 Artemia/day. Our study suggests that (a) G. aequicauda is an omnivorous species and can significantly suppress populations of its prey. (b) The two experimental approaches used to study the feeding of gammarids on Artemia produced similar results, and both may be used to quantitatively assess relations in a “prey–predator” system. (c) The abundance of predators and prey, as well as the experimental vessel volume, may influence the feeding rate. (d) The presence of plant food resources such as the leaves of Ruppia does not influence on the predatory feeding rate of G. aequicauda. (e) The rate of prey consumption by G. aequicauda is not constant and depends nonlinearly on prey and predator abundance. (f) Cannibalism occurs in the presence of plant resources only, but not in the presence of Artemia. (g) Other gammarids react to the capture of Artemia by one of them. They swim up to the successful individual and try to take some part of the prey.
The melatonin receptor antagonist luzindole induces the activation of cellular stress responses and decreases viability of rat pancreatic stellate cells
Authors: Estaras Matias , Marchena Ana M. , Fernandez‐Bermejo Miguel , Mateos Jose M. , Vara Daniel , Roncero Vicente , Salido Gines M. , Gonzalez Antonio 
Published: June 2020

AbstractIn this study, we have examined the effects of luzindole, a melatonin receptor‐antagonist, on cultured pancreatic stellate cells. Intracellular free‐Ca2+ concentration, production of reactive oxygen species (ROS), activation of mitogen‐activated protein kinases (MAPK), endoplasmic reticulum stress and cell viability were analyzed. Stimulation of cells with the luzindole (1, 5, 10 and 50 μm) evoked a slow and progressive increase in intracellular free Ca2+ ([Ca2+]i) towards a plateau. The effect of the compound on Ca2+ mobilization depended on the concentration used. Incubation of cells with the sarcoendoplasmic reticulum Ca2+‐ATPase inhibitor thapsigargin (1 μm), in the absence of Ca2+ in the extracellular medium, induced a transient increase in [Ca2+]i. In the presence of thapsigargin, the addition of luzindole to the cells failed to induce further mobilization of Ca2+. Luzindole induced a concentration‐dependent increase in ROS generation, both in the cytosol and in the mitochondria. This effect was smaller in the absence of extracellular Ca2+. In the presence of luzindole the phosphorylation of p44/42 and p38 MAPKs was increased, whereas no changes in the phosphorylation of JNK could be noted. Moreover, the detection of the endoplasmic reticulum stress‐sensor BiP was increased in the presence of luzindole. Finally, viability was decreased in cells treated with luzindole. Because cellular membrane receptors for melatonin have not been detected in pancreatic stellate cells, we conclude that luzindole could exert direct effects that are not mediated through its action on melatonin membrane receptors.
A Drug Holiday Reduces the Frequency and Severity of Medication-Related Osteonecrosis of the Jaw in a Minipig Model
Authors: Otto Sven , Pautke Christoph , Arens Daniel , Poxleitner Philipp , Eberli Ursula , Nehrbass Dirk , Zeiter Stephan , Stoddart Martin J 
Published: June 2020

ABSTRACT
Treatment of medication-related osteonecrosis of the jaw (MRONJ) is challenging and no clear consensus has been achieved. This study investigated preventive measures recommended for tooth extractions under antiresorptive (AR) treatment and the role of discontinuation of AR therapy to avoid the onset of MRONJ in a minipig model. Thirty-six Göttingen minipigs were divided into four groups. Group 1 (negative control): tooth extractions but no zoledronate (ZOL). Group 2 (positive control): weekly ZOL infusions for 12 weeks followed by tooth extractions without wound management followed by 8 weeks of ZOL treatment. Group 3: weekly ZOL infusions for 12 weeks followed by tooth extractions; surgical wound management (resection of sharp bone edges, mucoperiosteal coverage); and continuation of ZOL infusions for 8 weeks plus antibiotic treatment. Group 4: 12 weeks of ZOL infusions followed by a drug holiday for 6 weeks. Tooth extractions with preventive wound management followed by antibiotic treatment for 8 weeks but no ZOL infusions. Jawbones were subjected to macroscopic, radiological (CT and micro-CT) and histopathological investigations. No clinical cases of MRONJ were observed in the negative group, in the positive control all animals developed MRONJ. Group 3 developed MRONJ in 83% of cases. With a drug holiday, 40% developed MRONJ in areas of tooth extraction. This is the first large animal model that reduces the occurrence of MRONJ following tooth extraction by the implementation of a drug holiday combined with antibiotic prophylaxis and smoothening of sharp bony edges. © 2020 The Authors. Journal of Bone and Mineral Research published by American Society for Bone and Mineral Research..
A note on 3‐partite graphs without 4‐cycles
Authors: Lv Zequn , Lu Mei , Fang Chunqiu 
Published: June 2020

AbstractLet be a cycle of order 4. Write for the maximum number of edges in a balanced 3‐partite graph whose vertex set consists of three parts, each has vertices that have no subgraph isomorphic to . In this paper, we show that , where and is a prime number. Note that from Tait and Timmons's works. Since for every integer , one can find a prime such that , we obtain that .
Challenging the role of challenge in intensive short‐term dynamic psychotherapy for social anxiety disorder: A randomized controlled trial
Authors: Rahmani Fateh , Abbass Allan , Hemmati Azad , Ghaffari Niloofar , Rezaei Mirghaed Sahar 
Published: June 2020

AbstractObjectivesIntensive short‐term dynamic psychotherapy (ISTDP) requires the technique of challenge to defenses when treating resistant patients. As the technique of challenge is difficult for some therapists to practice, it leads us to question whether challenge can be replaced by clarification of defenses without losing treatment effectiveness. This study compared ISTDP with two different technical emphases while treating social anxiety disorder (SAD).MethodForty‐two subjects with DSM‐5 SAD were randomly assigned to either a waitlist control, 10 sessions of ISTDP with the use of challenge or 10 sessions of ISTDP without the use of challenge.ResultsISTDP led to significant, sustained symptom reduction on the Liebowitz Social Anxiety Scale (LSAS‐SR) compared with the control group. There were no significant outcome differences between standard ISTDP and ISTDP where challenge was restricted.ConclusionISTDP is efficacious for SAD. ISTDP may be effective for SAD without the use of challenge elements.
Nail‐biting, scab‐picking, and tattooing as nonsuicidal self‐injury (NSSI): A deviant case series analysis of the proposed NSSI disorder diagnostic criteria
Authors: Mann Adam J. D. , Van Voorhees Elizabeth E. , Patel Tapan A. , Wilson Sarah M. , Gratz Kim L. , Calhoun Patrick S. , Beckham Jean C. , Kimbrel Nathan A. 
Published: June 2020

AbstractObjectiveThis study explored the boundaries of the proposed diagnostic criteria for nonsuicidal self‐injury disorder (NSSID) as outlined in the Conditions for Further Study section of the Diagnostic and Statistical Manual, Fifth Edition. We sought to falsify the exclusion of certain NSSI behaviors from a diagnosis of NSSID (Criterion D), arguing that these exclusions are inconsistent with the broader phenomenology of the disorder outlined in the other criteria. MethodWe describe three case studies involving NSSI (Case 1: scab‐picking; Case 2: nail‐biting; Case 3: tattooing) that cannot be diagnosed as NSSID because the behaviors are explicitly listed in Criterion D.ResultsDespite exclusion as a relevant NSSI behavior per Criterion D, each examined behavior is consistent with the intentionality, functionality, and distress/impairment of NSSID that represent core features of the disorder.ConclusionThe case studies presented here suggest that Criterion D should be revised or removed from the NSSID criteria.
Pain centrality mediates pain self‐efficacy and symptom severity among individuals reporting chronic pain
Authors: Sucher Jillian , Quenstedt Stella R. , Parnes McKenna F. , Brown Adam D. 
Published: June 2020

AbstractChronic pain is consistently associated with the presence of mental health disorders. Although previous research has shown relations between low levels of self‐efficacy with chronic pain severity as well as comorbid mental health symptoms, the link between self‐efficacy and mental health symptoms in chronic pain is not well understood. This study examined whether pain centrality, the extent to which pain is viewed as central to self‐identity, may underlie these associations. Individuals with a diagnosis of chronic pain (N = 89) recruited through MTurkcompleted self‐report measures including demographics, self‐efficacy, pain centrality, pain severity, depression, and anxiety. Pain severity was associated with higher levels of pain centrality, depression, anxiety, and lower levels of self‐efficacy. Path analysis demonstrated pain centrality significantly mediated the relationship between self‐efficacy and pain severity, depression, and anxiety. Future studies would benefit from testing whether modifying pain centrality beliefs shift perceptions of control as well as pain and psychological outcomes.