bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impact of Embryo Transfer on the phenotype of the offspring in pigs
Authors: Paris-Oller Evelynne , Matás Carmen , Romar Raquel , Lopes Jordana Sena , Gadea Joaquín , Cánovas Sebastián , Coy Pilar 
Published: July 2020

Abstract
BackgroundThe increased use of assisted reproductive technologies led to a higher risk of specific alterations in the offspring, as already described in human and other mammalian species. Nonetheless, it is yet to be totally understood how and which specific techniques are inducing these changes. The use of animal models may represent an advantage to find out answers to these questions due to the minimization of confounding factors. The pig is not only a great human reproductive model, but it is also of interest in the livestock industry to develop further technologies that may enhance meat production. This study was designed to decipher the impact of one of these technologies, namely embryo transfer, on mother´s reproductive performance and on the phenotype of the offspring in pigs.ResultsEmbryo transfer of in vivo derived embryos (ET) showed similar results in terms of pregnancy length, litter size and total litter weight per sow than sows artificially inseminated (AI). Bodyweight at birth was higher for ET-females than AI-females, but this difference was no longer observed at day 15 of postnatal life. Some of the major findings after haematological analyses were that concentration of red blood cells, haemoglobin and haematocrit in female-ET piglets at day 3 showed higher values than AI-females. Additionally, higher concentration of white blood cells was found at day 3 in both ET-derived piglets (males and females) in comparison to AI-piglets. Biochemical analysis showed a higher level of albumin at day 3 for ET-derived male piglets than AI but ET-females showed lower levels of bilirubin than AI in the same day. Despite the statistical differences between groups, all values were within the normal ranges, so that we cannot give them any clinical significance.ConclusionsPiglets derived from in vivo embryo transfer seem to be phenotypically similar, which provides evidence that the embryo transfer procedure is a safe technique, without relevant health implications to the offspring. Additionally, these findings may be of interest to the pig industry regarding the reference values provided for haematological and biochemical parameters in this species.
Predictive and Prognostic Role of Tumor Infiltrating Lymphocytes in Breast Cancer Patients With Different Molecular Subtypes: a Meta-Analysis
Authors: Gao Zhao-hua , Li Cun-xin , Liu Ming , Jiang Jia-yuan 
Published: July 2020

Abstract
Background Whether TILs plays different roles in different molecular subtypes breast cancer remains unknown. The prognostic and predictive value of TILs in different molecular subtypes breast cancer is still controversy. The aim of our meta-analysis is to assess the prognostic and predictive value of TILs in different molecular subtypes breast cancer by summarizing all relevant studies including multivariate analysis.MethodsPubMed, Embase, EBSCO, ScienceDirect, Cochrane Database and the Web of Science were comprehensively retrieved (until March 2020). Hazard ratio (HR), odds ratio (OR) and their 95% confidence intervals (CI) were used as effect measures to perform our meta-analysis. Random effect model was used. Stata software, version 15 (2017) (Stata Corp, College Station, TX, USA) was used to carried out statistical analysis. ResultsThirty-three studies including 18170 eligible breast cancer patients were analyzed. The meta-analysis showed that patients with high TILs expression were significantly correlated with increased pCR after neoadjuvant chemotherapy in HER2 enriched molecular subtype (OR = 1.137, 95% CI [1.061~1.218], p <0.001) and TNBC molecular subtype breast cancer (OR = 1.120, 95% CI [1.061~1.182], p <0.001). But, patients with high TILs expression were not significantly related to high pCR in luminal molecular subtype breast cancer after neoadjuvant chemotherapy (OR =1.154, 95% CI [0.789~1.690], p = 0.460). We carried out this meta-analysis on HR for OS and DFS to assess the prognostic value of TILs in breast cancer with different molecular subtypes more deeply. Our meta-analysis confirmed that high TILs had relationship with a significantly improved DFS in HER2 enriched molecular subtype [HR=0.940, 95%CI (0.903~0.979), p=0.003] and TNBC molecular subtype breast cancer patients [HR=0.907, 95%CI (0.862~0.954), p<0.001]. However, high TILs was not correlated with a significantly better DFS in luminal molecular subtype breast cancer patients [HR=0.998, 95%CI (0.977~1.019), p=0.840]. Furthermore, the results confirmed that high TILs had significant relationship with a better OS in HER2 enriched molecular subtype [HR=0.910, 95%CI (0.866~0.957), p <0.001] and TNBC molecular subtype breast cancer patients [HR=0.869, 95%CI (0.836~0.904), p <0.001]. Conversely, the summarized results indicated that high TILs was significantly correlated with a poor OS in luminal molecular subtype breast cancer patients [HR=1.077, 95%CI (1.016~1.141), p=0.012].ConclusionsOur meta-analysis confirms that high TILs is correlated with favourable survival and predicts pathological complete response in breast cancer patients with TNBC molecular subtypes and HER2-enriched molecular subtypes.
HRD1 Elicitation of An Anti-Warburg Effect by Targeting PFKP to Inhibit Breast Cancer Growth and Progression
Authors: Fan Ya , Jia Wang , Xu Yuemei , Wang Yipin , Song Tao , Liang Xiubin , Feng Jin , Su Dongming 
Published: July 2020

Abstract
Background: Our previous studies have shown that the E3 ubiquitin ligase of HMG-CoA reductase degradation 1 (HRD1) functions as a tumor suppressor, as overexpression of HRD1 suppressed breast cancer proliferation and invasion. However, its role in breast cancer cell glucose metabolism was unclear. Here, our aim was to uncover the role and molecular mechanisms of HRD1 in regulating aerobic glycolysis in breast cancer. Methods: The effect of HRD1 on robic glycolysis in breast cancer cells were assessed. Then the proliferation, colony formation ability, invasion and migration of breast cancer cells were evaluated. The relationship between HRD1 and PFKP was validated by Mass spectrometry analysis, immunofluorescence and co-immunoprecipitation. The level of PFKP ubiquitination was measured using ubiquitylation assay. Furthermore, the tumor growth in vivo were observed by subcutaneous tumorigenesis in nude mice. Results: We found that upregulation of HRD1 clearly decreased aerobic glycolysis, and subsequently inhibited breast cancer proliferation and invasion. Mass spectrometry analysis results revealed a large HRD1 interactome, which included PFKP (platelet isoform of phosphofructokinase), a critical enzyme involved in the Warburg Effect in breast cancer. Mechanistically, HRD1 interacted and colocalized with PFKP in the cytoplasm, targeted PFKP for ubiquitination and degradation, and ultimately reduced PFKP expression and activity in breast cancer cells. Conclusions: Our findings reveal a new regulatory role of HRD1 in Warburg effect and provide a key contributor in breast cancer metabolism.
Microenvironmental Hypoxia Induces Dynamic Changes in Lung Cancer Synthesis and Secretion of Into Extracellular Vesicles
Authors: Tse Shun Wilford , Tan Chee Fan , Park Jung Eun , Gnanasekaran JebaMercy , Gupta Nikhil , Low Jee Keem , Yeoh Kheng Wei , Chng Wee Joo , Tay Chor Yong , McCarthy Neil , Lim Sai Kiang , Sze Siu Kwan 
Published: July 2020

Abstract
Background: Extracellular vesicles (EVs) mediate critical intercellular communication within healthy tissues, but are also exploited by tumour cells to promote angiogenesis, metastasis, and host immunosuppression under hypoxic stress. We hypothesize that oxygen starvation in developing tumours induces specific hypoxia-sensitive proteins for packing into small EVs to modulate its microenvironment for cancer progression and enhance malignancy. Methods: We employed a heavy isotope pulse/trace quantitative proteomic approach to study hypoxia-sensitive EVs proteins (HSEPs) in hypoxic A549 lung adenocarcinoma cells derived small EVs (<200 nm). Proteomics data mining and pathway analysis were used to reveal potential roles of the HSEPs in enhancing tumour cell progression and in modulating host immunity. Functional clustering was applied to study enhanced EVs biogenesis and secretion in hypoxic cancer cells. Subsequent biochemical functional assays were performed in A549 and H1299 lung cancer cells to validate the hypoxic cancer-derived EVs in promoting cancer progression.Results: Results revealed that hypoxia stimulated cancer cells to synthesize EVs proteins involved in enhancing tumour cell proliferation (NRSN2, WISP2, SPRX1, LCK), metastasis (GOLM1, STC1, MGAT5B), stemness (STC1, TMEM59), angiogenesis (ANGPTL4), and suppressing host immunity (CD70). In addition, functional clustering analyses revealed that tumour hypoxia was strongly associated with rapid synthesis and EV loading of lysosome-related hydrolases and membrane-trafficking proteins to enhance EVs secretion. Moreover, lung cancer-derived EVs were also enriched in signalling molecules capable of inducing epithelial-mesenchymal transition in recipient cancer cells to promote their migration and invasion. Conclusion: Together, these data indicate that lung cancer-derived EVs can act as paracrine/autocrine mediators of tumorigenesis and metastasis in hypoxic microenvironments. Tumour EVs may therefore offer novel opportunities for useful biomarkers discovery and therapeutic targeting of different cancer types and at different stages according to microenvironmental conditions.
Antibiotic susceptibility profile of bacterial pathogens isolated from febrile children under 5 years of age in Nanoro, Burkina Faso.
Authors: BONKO Massa dit Achille , Tahita Marc Christian , Kiemde Francois , Lompo Palpouguini , Yougbaré Sibidou , Some Athanase M. , Tinto Halidou , Mens Petra F. , Menting Sandra , Schallig Henk D. F. H. 
Published: July 2020

Abstract
Background: The curative power of antimicrobials is severely threatened due to emerging resistance to first-line antibiotics worldwide. With a limited reserve of antibiotics, increasing antimicrobial resistance has become a global concern, but there is a paucity of such data in Burkina Faso, and the West African region in general. Therefore, this study aims to determine the antibiotic susceptibility profile of bacterial species isolated from febrile children under 5 years of age in Nanoro (Burkina Faso). Methods: Clinical specimens (blood, stool, and urine) were collected from 1099 febrile children attending the peripheral health facilities and the referral hospital in Nanoro. Bacterial isolates from these clinical specimens were assessed for their susceptibility against commonly used antibiotics by standard disc diffusion procedure and minimal inhibitory concentration method (when appropriate). Results: In total, 141 bacterial strains were recovered from 127 febrile children of which 65 strains were isolated from blood, 65 from the stool, and 11 from urine. Predominant bacterial isolates were Salmonella species (56.7%; 80/141) followed by Escherichia coli (33.3%; 47/141). Antibiotic susceptibility testing revealed Salmonella species were highly resistant to ampicillin (70%; 56/80), trimethoprim-sulfamethoxazole (65%; 52/80), and chloramphenicol (63.8%; 51/80). E. coli isolates were highly resistant to trimethoprim-sulfamethoxazole (100%), ampicillin (100%), ciprofloxacin (71.4%; 10/14), amoxicillin-clavulanate (64.3%; 9/14), ceftriaxone (64.3%; 9/14), and gentamycin (50%; 7/14). Moreover, 7 out of 14 E. coli isolates were producers of the ß-lactamase enzyme, suggesting multi-drug resistance against b-lactam as well as non-b-lactam antibiotics. S. pneumoniae isolates were fully resistant to tetracycline and 50% to penicillin G. Multi-drug resistance was observed in 54.6% (59/108) of the isolates of which 56 (54.9%) were Gram-negative bacteria and 3 (50.0%) Gram-positive bacteria.Conclusions: The antibiotic susceptibility profiling showed an alarming high resistance to commonly used antibiotics to treat bacterial infections in the study region. The work prompts the need to expand antibiotic resistance surveillance studies in Burkina Faso, and probably the whole region (West Africa). Moreover, it implies the need of a revision of the antibiotic-treatment guidelines by the Ministry of Health in Burkina Faso to avoid further development of resistance.
An Exploratory Model of Competitive Advantage through Dynamic Capabilities and Differentiation Approach for Knowledge-Based Companies
Authors: Khaligh Alireza Abdolhosseini , Haghighi Mohammad , Nazari Mohsen , Hosseini Hamid Khodadad 
Published: July 2020

Abstract
The competitive struggles in high-tech environment have featured the necessity to infer how competitive advantage is gained. Dynamic capabilities emphasize the nature of future competition, acceleration in innovation growth, and the role of strategic management in adaption, integration, and reconfiguration of organizational skills, resources, and competences toward shifting environment. This inquiry is based on an inductive approach. The qualitative research is primarily started with exploratory purpose through grounded theory strategy. Then, it is continued with quantitative type of study, explanatory and descriptive purposes through survey and correlational research strategies. The probe concepts and variables are analyzed in 30 top knowledge-based companies of Iran working on electronics and informatics field. The results indicate that achieving knowledge-centricity main phenomenon through dynamic capabilities requires presence of value creation on the basis of resource orientation together with competences. The knowledge-based companies can reach the summit and sustainable success in the knowledge-centricity main phenomenon when they consider two specialized paths related to the differentiation and knowledge-based strategies. Environmental cognition, knowledge management, knowledge approaches, branding and brand management, and strategic agility are identified with a positive and significant effect on both strategies.
AGENTES PÚBLICOS DO SETOR DE LICITAÇÕES E A PSICODINÂMICA DO TRABALHO VIVÊNCIAS DE MEDO E SATISFAÇÃO
Authors: Bonora Rosicler Mari Camargo , D'Oliveira Érika Pessanha 
Published: July 2020

A psicodinâmica do trabalho tem se tornado um estudo bastante frequente, já que versa sobre doença/saúde mental. Este artigo dedica-se à análise das vivências de prazer e sofrimento psíquico no cotidiano profissional dos agentes públicos limitados pelo medo da interpretação equivocada dos atos públicos pelos órgãos controladores e as estratégias de enfrentamento e de construção da saúde mental deste trabalhador. No primeiro momento utiliza-se um relato de experiência, onde se constatam as vivências subjetivas edificadas no exercício das funções de servidores que atuam no departamento de licitações de uma instituição pública. A partir do entendimento da indissociabilidade entre trabalho e vivências subjetivas, analisaram-se relatos sobre o cotidiano profissional que produz efeitos sobre os próprios sujeitos. Esses servidores convivem com as imposições das vivências de sofrimento no trabalho, ao tempo que procuram sentir prazer no meio profissional. A Psicodinâmica do Trabalho foi a abordagem adotada para análise das entrevistas semiestruturadas com servidores de dois órgãos distintos, especificamente do setor de licitações. O enfoque da análise foi o levantamento das vivências de prazer e sofrimento psíquico no cotidiano profissional dos agentes públicos limitado pelo medo da má interpretação de seus atos pelos órgãos controladores. A análise dos resultados mostrou que os impactos gerados pelo medo causam o bloqueio e interferem diretamente nas decisões dos servidores, deixando de agir em prol a sociedade.
Cross-linked-substituted (esterified/etherified) starch derivatives as aqueous heavy metal ion adsorbent: a review
Authors: Akinterinwa Ayodele , Oladele Ebun , Adebayo Albert , Gurgur Emmanuel , Iyanu Omotunde Oluwatoyin , Ajayi Olubode 
Published: July 2020

AbstractStarch is a biopolymer with outstanding economic and environmentally friendly attributes which has driven technological innovations to enhance its applications in food and non-food industries. Starch is constituted by O-H groups with valency and electronic characteristics that can initiate adsorption of aqueous heavy metal ions (AHMIs). However, this can be enhanced using various modification sequences. A common procedure is the cross-linking and substitution of the O-H groups via esterification and/or etherification reactions to produce starch derivative adsorbents (SDAs) with improved structural and functional properties for adsorption of AHMIs. The efficiency of SDAs developed using these procedures depends on the botanical source of the native starch base, porosity and structural stability of the derivative (i.e. degree of cross-linking), substituted functional group(s), degree of substitution and the steric/conformation effects of the substituted groups. Many works have been done to optimize these factors, and this review highlighted some of the tailored procedures and the results obtained.
Role of badger setts in life of other carnivores
Authors: Sidorchuk Natalia V. , Maslov Michail V. , Rozhnov Vyacheslav V. 
Published: July 2020

A study of interspecific interactions of European (Meles meles) and Asian (M. leucurus) badgers with other carnivores at badger setts was carried out in Darwin Reserve (European part of Russia) and in Ussuriisk Reserve (Russian Far East) in 2006-2011. We used camera traps for the registration of visits of carnivore mammals to the badger setts. Overall, 11 species were recorded. In both reserves, badger setts attract carnivore species during the whole year. Some predators visit badger setts regularly. The visitors can be divided into two groups: species searching shelter, or searching prey. The first group includes raccoon dog Nyctereutes procyonoides and red fox Vulpes vulpes. Raccoon dog was the most frequent visitor in both study areas (34 visits in Darwin reserve and 73 in Ussuriisk reserve). The second group includes lynx Lynx lynx and wolf Canis lupus in Darwin reserve and Asiatic black bear Ursus thibetanus, brown bear U. arctos, yellow-throated marten Martes flavigula aterrima and lynx Lynx lynx in Ussuriisk reserve. Smaller predators are also included into the second group because they can find prey at badger setts too: leopard cat Prionailurus bengalensis euptilura, sable Martes zibellina and Siberian weasel Mustela sibirica in Ussuriisk reserve and European pine marten Martes martes in Darwin reserve. No cases of aggressive interactions between the badgers and the visitors were recorded. But we noted two cases of change of sett owners and one case when raccoon dog removed dead badger cubs from the sett.
Repurposing of Auranofin Against Bacterial Infections: An In silico and In vitro Study
Authors: Sharma Nidhi , Singh Arti , Sharma Ruchika , Kumar Anoop 
Published: July 2020

Aim:
The aim of the study was to find out the role of auranofin as a promising broadspectrum
antibacterial agent.

Methods:
In vitro assays (Percentage growth retardation, Bacterial growth kinetics, Biofilm formation
assay) and In silico study (Molegro virtual docker (MVD) version 6.0 and Molecular operating
environment (MOE) version 2008.10 software).

Results:
The in vitro assays have shown that auranofin has good antibacterial activity against Gram
positive and Gram negative bacterial strains. Further, auranofin has shown synergistic activity in
combination with ampicillin against S. aureus and B. subtilis whereas in combination with neomycin
has just shown additive effect against E. coli, P. aeruginosa and B. pumilus. In vivo results have
revealed that auranofin alone and in combination with standard drugs significantly decreased the
bioburden in the zebrafish infection model as compared to control. The molecular docking study
have shown good interaction of auranofin with penicillin-binding protein (2Y2M), topoisomerase
(3TTZ), UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosaminedeacetylase (3UHM), cell adhesion
protein (4QRK), β-lactamase (5CTN) and arylsulphatase (1HDH) enzyme as that of reference ligand
which indicate the multimodal mechanism of action of auranofin. Finally, MTT assay has
shown the non-cytotoxic effect of auranofin.

Conclusion:
In conclusion, auranofin in combination with existing antibiotics, could be developed
as a broad spectrum antibacterial agent; however, further studies are required to confirm its safety
and efficacy. This study provides the possibility of the use of auranofin apart from its established
therapeutic indication in combination with existing antibiotics to tackle the problem of resistance.
Bacterial Operational Taxonomic Units Replace the Interactive Roles of Other Operational Taxonomic Units Under Strong Environmental Changes
Authors: Kangabam Rajiv Das , Silla Yumnam , Goswami Gunajit , Barooah Madhumita 
Published: July 2020

Background:
Microorganisms are an important component of an aquatic ecosystem and
play a critical role in the biogeochemical cycle which influences the circulation of the materials and
maintains the balance in aquatic ecosystems.

Objective:
The seasonal variation along with the impact of anthropogenic activities, water quality,
bacterial community composition and dynamics in the Loktak Lake, the largest freshwater lake of
North East India, located in the Indo-Burma hotspot region was assessed during post-monsoon and
winter season through metagenome analysis.

Methods:
Five soil samples were collected during Post-monsoon and winter season from the Loktak
Lake that had undergone different anthropogenic impacts. The metagenomic DNA of the soil samples
was extracted using commercial metagenomic DNA extraction kits following the manufacturer’s instruction.
The extracted DNA was used to prepare the NGS library and sequenced in the Illumina
MiSeq platform.

Results:
Metagenomics analysis reveals Proteobacteria as the predominant community followed by
Acidobacteria and Actinobacteria. The presence of these groups of bacteria indicates nitrogen fixation,
oxidation of iron, sulfur, methane, and source of novel antibiotic candidates. The bacterial members
belonging to different groups were involved in various biogeochemical processes, including fixation
of carbon and nitrogen, producing streptomycin, gramicidin and perform oxidation of sulfur, sulfide,
ammonia, and methane.

Conclusion:
The outcome of this study provides a valuable dataset representing a seasonal profile
across various land use and analysis, targeting at establishing an understanding of how the microbial
communities vary across the land use and the role of keystone taxa. The findings may contribute to
searches for microbial bio-indicators as biodiversity markers for improving the aquatic ecosystem of
the Loktak Lake.