bluuebunny/crossref_metadata_2025_split huggingface dataset:
Adequate tetanus but poor diphtheria and pertussis response to a Tdap booster in adolescents with juvenile systemic lupus erythematosus
Authors: Peracchi Octávio AB , Nicácio Aline Alencar MF , Yamada Juliana , Len Claudio Arnaldo , Moraes-Pinto Maria Isabel de , Terreri Maria Teresa 
Published: November 2020

Background Reports on vaccine responses in immunocompromised patients, such as juvenile systemic lupus erythematosus (jSLE), have shown highly variable results. Objective To compare the immune response and safety after a Tdap booster in 26 jSLE patients and 26 matched healthy adolescents. Methodology: Adverse events and disease activity were evaluated. Lymphocyte immunophenotyping was performed by flow cytometry. Tetanus, diphtheria and pertussis toxin antibodies were assessed by ELISA; whole blood was stimulated with whole cell pertussis and supernatants were assessed for cytokines by xMAP. Results Both groups showed a similar frequency of adverse events. There was no evidence of disease reactivation after Tdap booster in the jSLE cohort. Both groups showed a significant increase in antibody titers for all three antigens on D14 and D28 (p < 0.001). jSLE patients had a significantly lower increase in diphtheria titers than the control group (p = 0.007). jSLE patients had a distinct titer increase of tetanus and pertussis antibodies when compared to controls (p = 0.004 and p < 0.001, respectively). There was a lower frequency of pertussis seroconversion in the jSLE group on D14 (p = 0.009), D28 (p = 0.023), D12m (p = 0.015) and D24m (p = 0.004). Cellular immune response to Bordetella pertussis showed significantly lower levels of IFNγ (p < 0.001) and higher levels of IL10, IL12, IL21 and TNFα in jSLE patients than controls. Conclusions jSLE patients had good response to Tdap booster dose for the tetanus antigen, but not for diphtheria and pertussis. This vaccine was safe in relation to adverse events and absence of disease reactivation.
Delineating Quality Indicators of Inpatient Psychiatric Hospitalization
Authors: Delaney Kathleen R. , Loucks Jeannine , Ray Richard , Blair Ellen W. , Nadler-Moodie Marlene , Batscha Catherine , Sharp David M. , Milliken Dani 
Published: November 2020

Background: Assuring quality care is critical to the well-being and recovery of individuals receiving inpatient psychiatric treatment, yet a comprehensive map of quality inpatient care does not exist. Aims: To isolate and describe quality elements of inpatient psychiatric treatment. Methods: A survey queried psychiatric inpatient nursing leaders on what they considered to be critical elements of quality. The survey was emailed to 40 American Psychiatric Nurses Association members, and 39 individuals responded. In the survey, participants were asked to comment on the importance of six dimensions of quality as well as quality indicators used on their units. Results: Data from this survey indicate how thought leaders conceptualized quality of inpatient care. A unifying philosophy of care was endorsed as a quality element as was structure that affords staff available time on the unit—engaging with patients. While staffing levels were viewed as important, the respondents commented on the nuances between staffing and quality. Participants endorsed the importance of involving individuals in their treatment planning as well as tapping into patients’ perspectives on the treatment experience. Conclusions: The participants’ responses compliment the quality literature and reinforce the need to develop a comprehensive map of quality elements. These elements interact in complex way, for instance, staffing, engagement, and teamwork is tied to the organizational structure and philosophy of care, which in turn facilitates consumer involvement in care. Thus, gauging the impact of quality on outcomes will demand consideration of the interaction of factors not just the linear relationship of one element to an outcome.
Effectiveness of laptop-based versus smartphone-based videoconferencing interaction on loneliness, depression and social support in nursing home residents: A secondary data analysis
Authors: Tsai Hsiu-Hsin , Cheng Ching-Yu , Shieh Wann-Yun 
Published: November 2020

Introduction The worldwide coronavirus disease 2019 pandemic re-emphasises the importance of videoconferencing in supporting interactions between nursing home residents and their family and friends. However, there is a scarcity of comparative studies on how modalities of conferencing programs impact health outcomes. The purpose of this study was to compare laptop-based with smartphone-based videoconferencing programs on nursing home residents’ perceptions of loneliness, depressive symptoms and social support. Methods This retrospective secondary analysis used data from two previous studies on the effectiveness of videoconferencing programs (laptop-based and smartphone-based) to improve outcomes of loneliness, depressive symptoms and social support (type and source) among nursing home residents. Generalised estimating equations compared differences from baseline measures with measures at 1 and 3 months between the two groups with and without adjusting for the effects of confounding variables. Results With the exception of age, there were no differences in demographics between participants in the laptop and smartphone groups. Neither changes from baseline in mean scores for loneliness nor depressive symptoms differed between groups. However, changes in mean scores from baseline between groups were significantly greater in the smartphone-based group compared with the laptop-based group for three types of social support: informational and appraisal at 1 and 3 months; and emotional at 3 months. Changes from baseline in mean scores for social support from friends (at 1 and 3 months) and other sources (at 3 months) were also significantly greater for the smartphone group compared with the laptop group. Discussion The two modalities of videoconferencing did not differ in effects on depressive symptoms or loneliness. However, smartphones had a greater effect on the type and source of social support compared with laptops. Whether the small screen of a smartphone reduces users’ anxiety and allows them to talk more on screen is worth studying.
Predictive Value of Midsagittal Tissue Bridges on Functional Recovery After Spinal Cord Injury
Authors: Pfyffer Dario , Vallotton Kevin , Curt Armin , Freund Patrick 
Published: November 2020

Background The majority of patients with spinal cord injury (SCI) have anatomically incomplete lesions and present with preserved tissue bridges, yet their outcomes vary. Objective To assess the predictive value of the anatomical location (ventral/dorsal) and width of preserved midsagittal tissue bridges for American Spinal Injury Association (ASIA) Impairment Scale (AIS) grade conversion and SCI patient stratification into recovery-specific subgroups. Methods This retrospective longitudinal study includes 70 patients (56 men, age: 52.36 ± 18.58 years) with subacute (ie, 1 month) SCI (45 tetraplegics, 25 paraplegics), 1-month neuroimaging data, and 1-month and 12-month clinical data. One-month midsagittal T2-weighted scans were used to determine the location and width of tissue bridges. Their associations with functional outcomes were assessed using partial correlation and unbiased recursive partitioning conditional inference tree (URP-CTREE). Results Fifty-seven (81.4%) of 70 patients had tissue bridges (2.53 ± 2.04 mm) at 1-month post-SCI. Larger ventral ( P = .001, r = 0.511) and dorsal ( P < .001, r = 0.546) tissue bridges were associated with higher AIS conversion rates 12 months post-SCI (n = 39). URP-CTREE analysis identified 1-month ventral tissue bridges as predictors of 12-month total motor scores (0.4 mm cutoff, P = .008), recovery of upper extremity motor scores at 12 months (1.82 mm cutoff, P = .002), 12-month pin-prick scores (1.4 mm cutoff, P = .018), and dorsal tissue bridges at 1 month as predictors of 12-month Spinal Cord Independence Measure scores (0.5 mm cutoff, P = .003). Conclusions Midsagittal tissue bridges add predictive value to baseline clinical measures for post-SCI recovery. Based on tissue bridges’ width, patients can be classified into subgroups of clinical recovery profiles. Midsagittal tissue bridges provide means to optimize patient stratification in clinical trials.
Towards a detailed comprehension of the inertisation processes of amphibole asbestos:in situhigh-temperature behaviour of fibrous tremolite
Authors: Ballirano Paolo , Pacella Alessandro 
Published: November 2020

AbstractThermal behaviour of fibrous tremolite from Maryland, USA has been investigatedin situup to breakdown temperature. Tremolite can be found both as primary constituent and as contaminant in Asbestos Containing Materials (ACMs). The products of breakdown are subcalcic diopside and calcium-rich clinoenstatite in a 2:1 ratio, traces of hematite plus minor silica-rich amorphous material. Thermal expansion follows a regular trend up to 723 K before the onset of Fe2+oxidation/OH–deprotonation which is completed at 1023 K. At 923 K the Fe3+migration starts towardsM(1) and the corresponding counter-migration of Mg toM(2) andM(3). AtTclose to structure breakdown,M(2) shows a significant site-scattering reduction possibly consistent with the occurrence of minor vacancies. In fully oxidised tremolite, Fe3+is allocated prevalently atM(1) and subordinately atM(3). As it is well-known thatM(1), along withM(2), is the most exposed octahedral site at the surface of amphiboles, most of the Fe3+is available for participating in the Fenton-like reactivity of oxidised tremolite, potentially making it dangerous for human health. This point should be properly taken into account in the evaluation of the safety of thermally decomposed tremolite-containing ACMs, in particular in the case of accidentally incomplete treatments.
How I manage cyclic thrombocytopenia
Authors: Kyrle Paul A. , Eichinger Sabine 
Published: November 2020

Abstract
Cyclic thrombocytopenia (CTP) is a rare disease, which is characterized by periodic fluctuation of the platelet count. The pathogenesis of CTP is unknown and most likely heterogeneous. Patients with CTP are almost always misdiagnosed as having primary immune thrombocytopenia (ITP). The interval between ITP and CTP diagnosis can be many years. CTP patients often receive ITP-specific therapies including corticosteroids, thrombopoietin receptor agonists, rituximab, and splenectomy, which are followed by a transient increase in platelet count that is wrongly attributed to treatment effect with inevitable “relapse.” CTP can be diagnosed by frequent platelet count monitoring, which reveals a typical pattern of periodic platelet cycling. An early diagnosis of CTP will prevent these patients from being exposed to possibly harmful therapies. The bleeding phenotype is usually mild and consists of mucocutaneous bleeding at the time when the platelet count is at its nadir. Severe bleeding from other sites can occur but is rare. Some patients respond to cyclosporine A or to danazol, but most patients do not respond to any therapy. CTP can be associated with hematological malignancies or disorders of the thyroid gland. Nevertheless, spontaneous remissions can occur, even after many years.
Sperm miRNAs— potential mediators of bull age and early embryo development
Authors: Wu Chongyang , Blondin Patrick , Vigneault Christian , Labrecque Rémi , Sirard Marc-André 
Published: November 2020

AbstractBackgroundSperm miRNAs were reported to regulate spermatogenesis and early embryonic development in some mammals including bovine. The dairy cattle breeding industry now tends to collect semen from younger bulls under high selection pressure at a time when semen quality may be suboptimal compared to adult bulls. Whether the patterns of spermatic miRNAs are affected by paternal age and/or impact early embryogenesis is not clear. Hence, we generated small non-coding RNA libraries of sperm collected from same bulls at 10, 12, and 16 months of age, using 16 months as control for differential expression and functional analysis.ResultsWe firstly excluded all miRNAs present in measurable quantity in oocytes according to the literature. Of the remaining miRNAs, ten sperm-borne miRNAs were significantly differentially expressed in younger bulls (four in the 10 vs 16 months contrast and six in the 12 vs 16 months contrast). Targets of miRNAs were identified and compared to the transcriptomic database of two-cell embryos, to genes related to two-cell competence, and to the transcriptomic database of blastocysts. Ingenuity pathway analysis of the targets of these miRNAs suggested potential influence on the developmental competence of two-cell embryos and on metabolism and protein synthesis in blastocysts.ConclusionsThe results showed that miRNA patterns in sperm are affected by the age of the bull and may mediate the effects of paternal age on early embryonic development.
Transcriptional response of Bacillus megaterium FDU301 to PEG200-mediated arid stress
Authors: Zhao Lei , Zhou Yanjun , Li Jianbei , Xia Yucheng , Wang Weiyun , Luo Xiuqi , Yin Juan , Zhong Jiang 
Published: November 2020

AbstractBackgroundFor microorganisms on a paper surface, the lack of water is one of the most important stress factors. A strain ofBacillus megateriumFDU301 was isolated from plaques on a paper surface using culture medium with polyethylene glycol 200 (PEG200) to simulate an arid condition. Global transcriptomic analysis ofB. megateriumFDU301 grown under normal and simulated arid conditions was performed via RNA-seq technology to identify genes involved in arid stress adaptation.ResultsThe transcriptome ofB. megateriumFDU301 grown in LB medium under arid (15% PEG200 (w/w)) and normal conditions were compared. A total of 2941 genes were differentially expressed, including 1422 genes upregulated and 1519 genes downregulated under arid conditions. Oxidative stress-responsive regulatory genesperR,fur, andtipAwere significantly upregulated, along with DNA protecting protein (dps), and catalase (katE). Genes related to Fe2+uptake (feoB), sporulation stage II (spoIIB,spoIIE,spoIIGA), small acid-soluble spore protein (sspD), and biosynthesis of compatible solute ectoine (ectB,ectA) were also highly expressed to various degrees. Oxidative phosphorylation-related genes (atpB, atpE, atpF, atpH, atpA, atpG,atpD,atpC) and glycolysis-related genes (pgk,tpiA,frmA) were significantly downregulated.ConclusionThis is the first report about transcriptomic analysis of aB. megateriumto explore the mechanism of arid resistance. Major changes in transcription were seen in the arid condition simulated by PEG200 (15%), with the most important one being genes related to oxidative stress. The results showed a complex mechanism for the bacteria to adapt to arid stress.
Characterization, validation, and cross-species transferability of EST-SSR markers developed from Lycoris aurea and their application in genetic evaluation of Lycoris species
Authors: Jiang Yumei , Xu Sheng , Wang Rong , Zhou Jiayu , Dou Jian , Yin Qian , Wang Ren 
Published: November 2020

AbstractBackgroundTheLycorisgenus includes many ornamentally and medicinally important species. Polyploidization and hybridization are considered modes of speciation in this genus, implying great genetic diversity. However, the lack of effective molecular markers has limited the genetic analysis of this genus.ResultsIn this study, mining of EST-SSR markers was performed using transcriptome sequences ofL. aurea, and 839 primer pairs for non-redundant EST-SSRs were successfully designed. A subset of 60 pairs was randomly selected for validation, of which 44 pairs could amplify products of the expected size. Cross-species transferability of the 60 primer pairs amongLycorisspecies were assessed inL. radiataHreb,L. sprengeriComes ex Baker,L. chinensisTraub andL. anhuiensis, of which between 38 to 77% of the primers were able to amplify products in theseLycorisspecies. Furthermore, 20 and 10 amplification products were selected for sequencing verification inL. aureaandL. radiatarespectively. All products were validated as expected SSRs. In addition, 15 SSRs, including 10 sequence-verified and 5 unverified SSRs were selected and used to evaluate the genetic diversity of sevenL. radiatalines. Among these, there were three sterile lines, three fertile lines and one line represented by the offspring of one fertile line. Unweighted pair group method with arithmetic mean analysis (UPGMA) demonstrated that the outgroup,L. aureawas separated fromL. radiatalines and that the sevenL. radiatalines were clustered into two groups, consistent with their fertility. Interestingly, even a dendrogram with 34 individuals representing the sevenL. radiatalines was almost consistent with fertility.ConclusionsThis study supplies a pool of potential 839 non-redundant SSR markers for genetic analysis ofLycorisgenus, that present high amplification rate, transferability and efficiency, which will facilitate genetic analysis and breeding program inLycoris.
A comparison between two different definitions of contrast-associated acute kidney injury for long-term mortality in patients with diabetes undergoing coronary angiography: a prospective cohort study
Authors: Lun Zhubin , Lei Li , Zhou Dianhua , Ying Ming , Liu Liwei , Chen Guanzhong , Liu Jin , He Yibo , Li Huanqiang , Huang Zhidong , Yang Yongquan , Ye Jianfeng , Liu Yong 
Published: November 2020

AbstractBackgroundThe definitions of contrast-associated acute kidney injury (CA-AKI) are diverse and have different predictive effects for prognosis, which are adverse for clinical practice. Few articles have discussed the relationship between these definitions and long-term prognosis in patients with diabetes.MethodsA total of 1154 diabetic patients who were undergoing coronary angiography (CAG) were included in this study. Two definitions of CA-AKI were used: CA-AKIAwas defined as an increase ≥ 0.3 mg/dl or > 50% in serum creatinine (SCr) from baseline within 72 h after CAG, and CA-AKIBwas defined as an increase ≥ 0.5 mg/dl or > 25% in SCr from baseline within 72 h after CAG. We used Cox regression to evaluate the association of these two CA-AKI definitions with long-term mortality and calculate the population attributable risks (PARs) of different definitions for long-term prognosis.ResultsDuring the median follow-up period of 7.4 (6.2–8.2) years, the overall long-term mortality was 18.84%, and the long-term mortality in patients with CA-AKI according to both CA-AKIAand CA-AKIBcriteria were 36.73% and 28.86%, respectively. We found that CA-AKIA(HR: 2.349, 95% CI 1.570–3.517,p = 0.001) and CA-AKIB(HR: 1.608, 95% CI 1.106–2.339,p = 0.013) were associated with long-term mortality. The PARs were the highest for CA-AKIA(31.14%), followed by CA-AKIB(14.93%).ConclusionsCA-AKI is a common complication in diabetic patients receiving CAG. The two CA-AKI definitions are significantly associated with a poor long-term prognosis, and CA-AKIA, with the highest PAR, needs more clinical attention.
Prevalence and incidence of cognitive impairment in an elder Portuguese population (65–85 years old)
Authors: Pais Ricardo , Ruano Luís , Moreira Carla , Carvalho Ofélia P. , Barros Henrique 
Published: November 2020

Abstract
Background
The increase in average life expectancy increases the risk of illness and frailty in the elderly, especially in the cognitive arena. This study has the objective to estimate the prevalence and incidence of cognitive impairment, in a representative sample of 65 to 85 years old followed for a mean period of 6-years.

Methods
Subjects aged 65–85 years (n = 586) were screened at baseline (1999–2004) to estimate the prevalence of cognitive impairment using the Mini-Mental State Examination. A total of 287 individuals with a normal MMSE at baseline were reassessed after 6.2 mean years (± 4.30 years) to evaluate the incidence of cognitive impairment, defined as scoring below the age and education-adjusted MMSE cut-off points adapted for the Portuguese population. We did not exclude Dementia.

Results
The baseline prevalence of cognitive impairment was 15.5% (95% CI: 12.7–18.7). Higher in women (18.9%; 95% CI: 14.9–23.3), that in men (10.4%; 95% CI: 6.7–15.1). Increased with age and was highest for participants without any schooling. The overall incidence rate was 26.97 per 1000 person-years; higher in women (33.8 per 1000 person-years) than in men (18.0 per 1000 person-years). Higher for the oldest participants and those with no schooling. Taking the standard European population, we estimated a prevalence of 16.5% and an incidence of 34.4 per 1000 person-years.

Conclusion
The prevalence of cognitive impairment in Portugal is within the estimated interval for the European population, and the incidence is lower than for the majority of the European countries. Women, senior and elders without education have a higher risk of cognitive impairment. In our sample, neither employment nor marital status has a significant effect on cognitive impairment.
Risk factors associated with death in infants <120 days old with severe pertussis: a case-control study
Authors: Liu Cong , Yang Lin , Cheng Yuwei , Xu Hongmei , Xu Feng 
Published: November 2020

AbstractBackground and purposePertussis is a serious infectious disease in young infants, and severe cases frequently cause death. Our study explored risk factors for death from severe pertussis.MethodA case-control study of infants with severe pertussis admitted to the paediatric intensive care unit (PICU) in the Children’s Hospital of Chongqing Medical University, China, from January 1, 2013, to June 30, 2019, was conducted. Pertussis was confirmed by clinical features and laboratory examinations. Severe pertussis was defined as patients with pertussis resulting in PICU admission or death. To understand the risk factors for death, we compared fatal and nonfatal cases of severe pertussis in infants aged < 120 days by collecting clinical and laboratory data.ResultsThe participants included 63 infants < 120 days of age with severe pertussis. Fifteen fatal cases were confirmed and compared with 44 nonfatal severe pertussis cases, Four patients with termination of treatment were excluded. In the univariate analysis, the risk factors associated with death included apnoea (P = 0.001), leukocytosis (white blood cell (WBC) count≥30 × 109/L (P = 0.001) or ≥ 50 × 109/L (P = 0)), highest lymphocyte count (P = 0), pulmonary hypertension (P = 0.001), and length of PICU stay (P = 0.003). The multivariate analysis revealed that apnoea (OR 23.722, 95%CI 2.796–201.26,P = 0.004), leukocytosis (OR 63.708, 95%CI 3.574–1135.674,P = 0.005) and pulmonary hypertension (OR 26.109, 95%CI 1.800–378.809,P = 0.017) were significantly associated with death.ConclusionLeukocytosis and pulmonary hypertension exhibited the greatest associations with death in infants with severe pertussis admitted to the PICU. Vaccination is still the most effective protection method against pertussis.