bluuebunny/crossref_metadata_2025_split huggingface dataset:
Production of Vincristine and Vinblastine by the Endophytic Fungus Botryosphaeria laricina Strain (CRS1) is Dependent on Stimulating Factors Present in Catharanthus roseus
Authors: Bandara Chamara Janaka , Siriwardhana Asitha , Karunaratne Desiree Nedra , Ratnayake Bandara Bamunuarachi Mudiyanselage , Wickramasinghe Anura , Krishnarajah Shelomie Arulchelvi , Karunaratne Veranja 
Published: January 2020

Aims:To isolate vinca alkaloid producing endophytic fungi from Catharanthus roseus and the evaluation of the factors which enhance the vincristine production.Background:An endophytic fungus Botryosphaeria laricina (CRS1) isolated from Catharanthus roseus demonstrated vinca alkaloid production under certain conditions.Objective:o Understand the conditions under which the fungus was able to produce vincristine and vinblastine.Methods:Fungal isolates from C. roseus were grown in liquid culture and screened for alkaloid production. The strain (CRS1) producing catharanthine was sequenced and matched with GenBank. This isolated strain was studied for production of vinca alkaloids and the conditions required for vincristine and vinblastine production.Results:Eight endophytic fungi were isolated from the fresh aerial parts of C. roseus. Only CRS1, demonstrated catharanthine production. DNA sequencing of CRS1 gave a 100% match with the GenBank accession number, KC509580.1, which is related to the Botryosphaeria laricina strain JAS6. CRS1 produced only catharanthine when cultured in Czapek’s peptone liquid medium (CZ). Addition of C. roseus fresh plant extract (8.0 mL) to the culture medium (4.0 L) stimulated the production of catharanthine (3.2 mg), catharanthinic acid (0.3 mg), N-demethyl-vinblastine (0.3 mg), vinblastine (2.8 mg) and vincristine (2.4 mg). However, if the added plant extract was preheated (80 ˚C, for 15 min), no vinca alkaloids appeared other than catharanthine. To identify the active fractions of the plant extract stimulating vinca alkaloid production, the extract was dialyzed in buffer at 4 ˚C through 20 kDa MW cutoff membrane to separate into two fractions of molecules above and below 20 kDa MW. Only the fraction containing molecules above 20 kDa was able to transform catharanthine to vincristine and vinblastine. When the dialysis was performed in water instead of buffer, the larger fraction could only produce catharanthine and vinblastine. Other conditions such as the presence of light:dark (12:12 h), fructose (30.0 g L-1), glucose (30.0 g L-1), Cu2+(0.1 mM) ions, L-tryptophan (0.1%) and succinic acid (1%) did not induce alkaloid production.Conclusions:The catharanthine producing fungal strain B. laricina (CRS1) could only produce the two vinca alkaloids, vinblastine and vincristine from catharanthine in the presence of active components larger than 20 kDa MW present in the plant extract of C. roseus.
Comparative effectiveness of catheter ablation devices in the treatment of atrial fibrillation: a network meta-analysis
Authors: Gupta Dhiraj , Potter Tom De , Disher Tim , Eaton Kiefer , Goldstein Laura , Patel Leena , Grima Daniel , Velleca Maria , Costa Graça 
Published: January 2020

Atrial fibrillation (AF) ablation is most commonly performed using radiofrequency (RF) and cryoballoon (CB) catheters. Ablation Index is a novel lesion-quality marker associated with improved outcomes in RF ablation. Due to lack of direct comparative evidence between the latest generations of technologies, there is uncertainty regarding the best treatment option.
Aim:
To conduct a network meta-analysis to evaluate the comparative effectiveness of RF with Ablation Index to other catheter ablation devices in the treatment of AF.
Methods:
Searches for randomized and nonrandomized prospective comparative studies of ablation catheters were conducted in multiple databases. The outcome of interest was 12-month freedom from atrial arrhythmias after a single ablation procedure. Studies were grouped as high-, low- and unclear-quality based on study design and balanced baseline patient characteristics. Bayesian hierarchical network meta-analysis was conducted and results presented as relative risk ratios with 95% credible intervals (CrIs).
Results:
12 studies evaluating five different catheter ablation devices were included. Radiofrequency ablation with Ablation Index was associated with statistically significantly greater probability of 12-month freedom from atrial arrhythmias than Arctic Front (relative risk: 1.77; 95% CrI: 1.21–2.87), Arctic Front Advance™ (1.41; 1.06–2.47), THERMOCOOL™ (1.34; 1.17–1.48) and THERMOCOOL SMARTTOUCH™ (1.09; 1–1.3). Results were robust in multiple sensitivity analyses.
Conclusion:
Radiofrequency catheter with Ablation Index is superior to currently available options for 12-month freedom from atrial arrhythmias after AF ablation. This study provides decision-makers with robust, pooled, comparative evidence of the latest ablation technologies.
El impacto del salario mínimo y del empleo informal sobre el ingreso salarial en México
Authors: Gómez Rodríguez Tomás , Ríos Bolívar Humberto , Zambrano Reyes Adriana 
Published: January 2020

La presente investigación aborda el tema del impacto del salario mínimo y del empleo informal sobre las diferencias del ingreso salarial. Bajo este propósito, se plantea un Modelo de Censura que emplea datos codificados para un subconjunto de la población. La estimación del modelo se realizó con dos técnicas econométricas: Mínimos Cuadrados Ordinarios (MCO) con efectos fijos y el Método Generalizado de Momentos (GMM). En ambos casos, se emplearon microdatos de la ENOE por entidad federativa para el periodo 2005‑2014. Los resultados revelan que el salario mínimo amplia la brecha del ingreso salarial, mientras que el sector informal tiene un efecto reductor sobre las diferencias del ingreso salarial.
Methods of simulation mathematical modeling of the Russian derivatives market in modern times
Authors: Karpinskaya T. A. , Kudryavtsev O. E. 
Published: January 2020

Introduction. The paper is devoted to simulation modeling. Basic methods of the simulation mathematical modeling in the derivatives market are described. A group of realistic nonGaussian Levy processes that generalize the classical BlackScholes model is considered. The work objective is to study the most efficient methods of market forecasting, as well as the software implementation of the simulation mathematical modeling technique of the Russian derivatives market based on the Levy model. This research is relevant due to the demand for applications that simulate the dynamics of financial assets and evaluate options in realistic models of the derivatives market, allowing for jumps.Materials and Methods. Basic methods for forecasting the derivatives market, methods for determining the volatility rate at a known option price, are considered. The most effective types of Levy processes for the simulation mathematical modeling of the Russian derivatives market at the present stage are highlighted. The possibilities of the Java language for the implementation of mathematical methods are considered.Research Results. A program is developed in the Java programming language that implements the Levy mathematical model, which includes Gaussian and generalized Poisson processes. The program for calculating the mathematical method is created in the free integrated application development environment NetBeans IDE to work with any operating system.Discussion and Conclusions. The result of the simulation mathematical modeling analysis has shown that the most efficient methods in the derivatives market are those based on realistic non-Gaussian Levy processes. The software implementation of such mathematical methods can be used for educational purposes. The developed application has demonstrated high quality and speed of calculations using software resources.
INTEGRATED REPORTING DAN KEPUTUSAN INVESTASI: PRINSIP KONEKTIVITAS DAN TIME HORIZON INVESTASI
Authors: Dewi Murdiyati , Agustia Dian , Ardianto Ardianto 
Published: January 2020

Pelaporan terintegrasi adalah laporan yang berisi informasi keuangan dan non-keuangan yang mengkomunikasikan proses penciptaan nilai oleh perusahaan. Salah satu prinsip utama pelaporan terintegrasi adalah prinsip konektivitas. Penelitian ini bertujuan menguji pengaruh konektivitas dan time horizon investasi terhadap keputusan jumlah investasi. Penelitian ini menggunakan pendekatan eksperimen dengan desain faktorial 2 x 2 dengan responden 87 mahasiswa S1 akuntansi sebagai surrogate investor non profesional. Material kasus adalah bagian laporan terintegrasi salah satu perusahaan dalam database International Integrated Reporting Council, dengan modifkasi dalam bentuk diagram yang menggambarkan konektivitas informasi dan modal. Hipotesa diuji dengan ANOVA dan efek dari perlakuan dengan uji post hoc. Hasil menunjukkan bahwa investor non profesional dengan laporan terintegrasi berkonektivitas tinggi memiliki keputusan jumlah investasi lebih besar dari investor non profesional dengan laporan terintegrasi berkonektivitas rendah. Sebaliknya, investor non-profesional dengan laporan terintegrasi berkonektivitas tinggi dan investasi jangka panjang memutuskan jumlah investasi lebih besar dari investor non-profesional dengan laporan terintegrasi berkonektivitas rendah dan investasi jangka pendek, adalah tidak didukung. Hasil penelitian menunjukkan prinsip konektivitas dapat meningkatkan kemanfaatan keputusan dari laporan terintegrasi.
Dual Loading Of Primaquine And Chloroquine Into Liposome
Authors: Miatmoko A. , Salim H. R. , Zahro S. M. , Annuryanti F. , Sari R. , Hendradi E. 
Published: January 2020

Abstract
Primaquine (PQ) has long been recognized as the only effective drug in the treatment of hepatic stage malaria. However, severe toxicity limits its therapeutical application. Combining PQ with chloroquine (CQ) has been reported as enhancing the former’s efficacy, while simultaneously reducing its toxicity. In this study, the optimal conditions for encapsulating PQ-CQ in liposome, including incubation time, temperature and drug to lipid ratio, were identified. Furthermore, the effect of the loading combination of these two drugs on liposomal characteristics and the drug released from liposome was evaluated. Liposome is composed of HSPC, cholesterol and DSPE-mPEG2000 at a molar ratio of 55:40:5 and the drugs were loaded by means of the transmembrane pH gradient method. The particle size, ζ-potential and drug encapsulation efficiency were subsequently evaluated. The results showed that all liposome was produced with a similar particle size and ζ -potential. PQ and CQ could be optimally loaded into liposome by incubating the mixtures at 60°C for 20 minutes at a respective drug to lipid ratio of 1:3 for PQ and CQ. However, compared to single drug loading, dual-loading of PQ+CQ into liposome resulted in lower drug encapsulation and slower drug release. In conclusion, PQ and CQ can be jointly loaded into liposome with differing profiles of encapsulation and drug release.
Cardiac Imaging in Arrhythmias
Authors: Batâr Sergiu , Batâr Florina , Costache Victor , Manțiu Ioan 
Published: January 2020

Abstract
Cardiac arrhythmias occur frequently in valvular patients. In valvular patients the role of echocardiography is essential, due to prognostic variations. A common indication of CT coronarography is the evaluation of the coronary arteries, with the highlighting of the light coronary plaques, but also accurately measures the dimensions of the left atrium and the anatomy of the pulmonary veins. In the last years cardiac MRI (magnetic resonance imaging) has been used in the clinic and research media to establish the gold standard measurement for the volumes of heart cavities, their function and tissue structure. All these three methods are essential for the evaluation and treatment of the cardiac patient with important arrhythmia.
Experimental Testing of Some Polymer Materials
Authors: Muntean Diana , Oleksik Mihaela , Bologa Octavian 
Published: January 2020

Abstract
The research aims to test polymer materials as the plastic materials: UHMWPE and POM, to observe there characteristics, to determing how they can be used and what we can obtain from them. We wanted to see the strong and the weak values that characterize them, what kind of parts we can produce from them and in what case we can use it. By applying this tests is necessary in order to see real datas that can help you compare them, and lead you to conclusions. Using these methods of testing materials leads to the establishment of real properties of the material and to the establishment of new configurations necessary to be made to the manufactured parts.
Stability analysis of implicit fractional differential equation with anti–periodic integral boundary value problem
Authors: Zada Akbar , Waheed Hira 
Published: January 2020

Abstract
In this manuscript, we study the existence, uniqueness and various kinds of Ulam stability including Ulam–Hyers stability, generalized Ulam– Hyers stability, Ulam–Hyers–Rassias stability, and generalized Ulam–Hyers– Rassias stability of the solution to an implicit nonlinear fractional differential equations corresponding to an implicit integral boundary condition. We develop conditions for the existence and uniqueness by using the classical fixed point theorems such as Banach contraction principle and Schaefer’s fixed point theorem. For stability, we utilize classical functional analysis. The main results are well illustrated with an example.
Meat starter cultures: Isolation and characterization of lactic acid bacteria from traditional sausages
Authors: Laslo É. , György É. , Czikó A. 
Published: January 2020

Abstract
Fermented meat products represent an important segment of our alimentation. Obtaining these products is based on beneficial microorganism activity. In the case of traditional food products, these are commercial starters or autochthonous microflora. Fermentation of raw materials is mainly done by sugar metabolization of lactic acid bacteria (LAB). In addition, these microorganisms can have other beneficial properties too such as probiotic properties, antimicrobial compound production abilities, etc.
In order to meet consumer demands, starter cultures are continuously developed to produce high-quality, healthy, and tasty products, thus contributing to guaranteeing microbiological safety and to improving one or more sensory characteristics, technological, nutritional, or health properties of the fermented products. The aim of our research is to determine the technological properties of autochthonous lactic acid bacteria originated from commercial fresh sausages in order to select and use them as potential starter cultures in the meat industry. In our work, we determined the relevant characteristics (such as salt tolerance, proteolytic activity, antimicrobial activity, and antibiotic resistance) of bacteria isolated from 16 fresh sausages. Based on our results, the studied bacterial isolates originated from sausages could be potentially used as autochthonous meat starter cultures.
Evaluation of the microbiological quality of some fresh dairy products with Soleris® Automated System
Authors: Laslo É. , György É. , Mihok E. , Antal M. 
Published: January 2020

Abstract
The manufacture of dairy products is an important sector of the food industry. From milking to processing, a number of hygiene rules must be strictly followed. During processing, dairy products can be contaminated with different microorganisms, causing spoilage, infectious diseases, and alterations in the sensory characteristics. There are strict requirements for the quality assurance of milk products. In spite of this, there occur infections linked to milk and dairy product consumption. The analysis of the microbiological quality of these products is a health concern, and it also has an economic impact. The increase and development of the global market of processing technologies require rapid monitoring and controlling systems for food products. In our study, we investigated the microbiological quality of some fresh dairy products with the Soleris® test system. For instrument setting, calibration curves were realized with test bacterial strains. With known initial microbial load of the samples, the microbial growth versus time was measured by the above-mentioned system. The occurrence of Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus was evaluated in ten dairy products. Results obtained by the Soleris system showed that the system is efficient for this purpose. Calibration curves with high correlation coefficients permitted the quantitative determination of the aimed bacteria in the dairy product samples.
Entrepreneurial Spirit for the Whole School – Ways to Become an e.e.si-Entrepreneurship School
Authors: Lindner Johannes 
Published: January 2020

Abstract
All entrepreneurs of the future are at school today, the nature of their value-oriented education and their willingness to actively participate in business and society in the future is shaped by today’s learning. This article focuses on the development, the reasoning, the legal bases, the implementation and the future perspectives of the Austrian approach to Entrepreneurship Education with a specific focus on an Entrepreneurship School Certification. Entrepreneurship Education in schools involves a variety of factors: the learning program, the learning environment, the teachers with their passion, encouragement and appreciation of learners and the value-orientation of the organization, which ranges from corporate culture to networking with external school partners.
Self-Efficacy in Social Science
Authors: Pröbstl Gerlinde , Schmidt-Hönig Kerstin 
Published: January 2020

Abstract
In order to manage the challenges of the 21st century, the assessment of individual self-efficacy constitutes an important factor in the educational context; both on the personal as well as on the social and organisational level. This fact justifies the consideration of self-efficacy as a basic competence, whose development must be started on the primary level. Pupils and teachers realise the resources that contribute to the development of a successful self-concept: increased motivation, a courageous selection of tasks to be fulfilled, and the possible influence on future social and economic developments. During their formation pedagogues are learners and teachers at the same time. In their lectures they deal with Social Entrepreneurship Education and competences connected with it. They reflect their own potential to exert influence and develop strategies to support pupils/students in the development of their own self-concept. In accordance with current findings the re-formulation of the curriculum of primary and secondary level demonstrates a development towards conceptual learning in larger contexts, following the principles of self-guidance and activity-orientation.
Makerspaces as Social Innovation and Entrepreneurship Learning Environments: The DOIT Learning Program
Authors: Geser Guntram , Hollauf Eva-Maria , Hornung-Prähauser Veronika , Schön Sandra , Vloet Frank 
Published: January 2020

Abstract
Developing social innovation and entrepreneurship competences and skills of children and young people is on the agenda of European educational policy-makers. The European research and innovation project “DOIT – Entrepreneurial skills for young social innovators in an open digital world” suggests using makerspaces and tools, within schools and externally, to promote practice-based social innovation and entrepreneurial learning of children and young people. This article first gives an overview of different types of maker-spaces, addresses the concept of maker education, and highlights common development goals of such education with entrepreneurship education regarding particular attitudes and competences. The main part then describes the DOIT learning program that allows children and young people (6–16 years) to acquire skills and an entrepreneurial mind-set for turning creative ideas into potential social innovations. This program is currently trialed in DOIT pilots in different types of makerspaces in ten European countries. The article describes learning processes and outcomes that are promoted by the program with two examples that are different regarding the makerspaces, topics and other aspects. Some first experiences and lessons learned from these and other pilots are summarized.
Analysis of Internally Generated Goodwill Indicators: A Case Study of the Slovak Republic
Authors: Podhorska Ivana , Gajanova Lubica , Kliestikova Jana , Popescu Gheorghe H. 
Published: January 2020

Abstract

Background and purpose: Knowing key indicators of goodwill value can contribute to its effective management and growth of the market value of the enterprise. The purpose of this research is to identify individual goodwill indicators. The paper aim is to obtain potential indicators of enterprise goodwill under the conditions of the Slovak Republic.

Design/Methodology/Approach: Paper data included 11,483 financial statements of Slovak enterprises in 2017. The value of residual enterprise income represents the value of goodwill. Input data for the identification of goodwill indicators represented 15 financial-economic variables. Outliers in data were searched and removed through an interquartile range. Multicollinearity among input variables, by the coefficient of determination and variance inflation factor, was also analysed. A statistically significant correlation between goodwill and its potential indicator were tested by the significance test of the Pearson correlation coefficient and correlation matrixes.

Results: Research results reveal the existence of a statistically significant correlation between goodwill and 8 input variables, which represent its potential vital indicators.

Conclusion: Paper findings bring new possibilities for goodwill management, which may create an essential competitive advantage of a company. For the scientific community, the findings represent sources of potential goodwill indicators which can be used for the creation of the new model of goodwill valuation in future research.
Life satisfaction of the pregnant women
Authors: Skurzak Agnieszka , Zarajczyk Marta , Iwanowicz-Palus Grażyna , Korżyńska-Piętas Magdalena , Lewicka Magdalena , Wiktor Henryk 
Published: January 2020

Abstract

Introduction. Satisfaction with life is a general assessment of satisfaction with one’s own achievements and living conditions on many levels. This includes human cognitive processes, expressing the emotional state that occurs as a result of achieving a specific goal. According to Juczyński, the assessment of life satisfaction is the result of confronting one’s own situation with the adopted criteria. If the result of the comparison is positive, the implication is the feeling of satisfaction. Satisfaction with life of pregnant women is dependent on many factors, including: personality traits, emotions, sociodemographic, factors as well as numerous physiological and psychological changes occurring during pregnancy.

Aim. The aim of the study is to assess the level of life satisfaction of pregnant women depending on sociodemographic factors.

Material and methods. The research was conducted on 415 women hospitalized and receiving care in counseling centers for pregnant women. The research was carried out by means of a diagnostic survey using a standardized Satisfaction With Life Scale (SWLS) and an original questionnaire to collect demographic data.

Results. Based on the average assessment of life satisfaction, it was found that 48.43% (n = 201) of the surveyed pregnant women was characterized by a high degree of life satisfaction, 35.18% (n = 146) average, and 16.39% (n = 68) of the respondents experienced a low level of life satisfaction.

Conclusions. Sociodemographic factors conditioned the feeling of satisfaction with life of pregnant women. Pregnant women who had a greater sense of life satisfaction were: those in relationship rather than those who were single; women with higher education than those with basic, vocational or secondary education; pregnant women working mentally rather than those not working.