bluuebunny/crossref_metadata_2025_split huggingface dataset:
Red blood cells are superior to plasma for predicting subcutaneous trans fatty acid composition in beef heifers
Authors: Vahmani P. , Rolland D.C. , Block H.C. , Dugan M.E.R. 
Published: March 2020

The trans (t)-18:1 content in beef has become more of interest as partially hydrogenated vegetable oils are removed from foods. Predicting t-18:1 early in the feeding period would be useful if limitations are put on t-18:1 in beef. To determine which blood component is better related to backfat, proportions of t10-18:1 and t11-18:1 (vaccenic acid) were measured in heifer red blood cells (RBC) and plasma (N = 14) after 0, 28, 56, and 76 d on a barley-grain-based diet, and correlated with post-slaughter subcutaneous fat (SCF). Total t-18:1 declined in both RBC and plasma during late finishing (P < 0.05). At 28 d, t11-18:1 decreased and t10-18:1 increased in RBC and plasma (P < 0.05). By 76 d, t10-18:1 declined to 0 d levels. RBC and plasma t-18:1 compositions were highly correlated (t10-18:1, r ≥ 0.7, P ≤ 0.02; t11-18:1, r ≥ 0.51, P ≤ 0.06). Correlations with post-slaughter backfat were, however, consistently greater for RBC compared with plasma. The use of RBC t-18:1 composition may, therefore, be superior to plasma for predicting t-18:1 in SCF, and the length of finishing could be useful for manipulating t-18:1 in beef. The time required for changes in t18:1 in RBC to reflect in changes in SCF still, however, needs to be determined to establish optimal durations for beneficial modification.
Effect of Angiotensin II on ENaC in the Distal Convoluted Tubule and in the Cortical Collecting Duct of Mineralocorticoid Receptor Deficient Mice
Authors: Wu Peng , Gao Zhong‐Xiuzi , Zhang Dan‐Dan , Duan Xin‐Peng , Terker Andrew S. , Lin Dao‐Hong , Ellison David H. , Wang Wen‐Hui 
Published: March 2020

Background

Angiotensin II stimulates epithelial Na
+
channel (ENaC) by aldosterone‐independent mechanism. We now test the effect of angiotensin II on ENaC in the distal convoluted tubule (DCT) and cortical collecting duct (CCD) of wild‐type (WT) and kidney‐specific mineralocorticoid receptor knockout mice (KS‐MR‐KO).

Methods and Results

We used electrophysiological, immunoblotting and renal‐clearance methods to examine the effect of angiotensin II on ENaC in KS‐MR‐KO and wild‐type mice. High K
+
intake stimulated ENaC in the late DCT/early connecting tubule (DCT2/CNT) and in the CCD whereas low sodium intake stimulated ENaC in the CCD but not in the DCT2/CNT. The deletion of MR abolished the stimulatory effect of high K
+
and low sodium intake on ENaC, partially inhibited ENaC in DCT2/CNT but almost abolished ENaC activity in the CCD. Application of losartan inhibited ENaC only in DCT2/CNT of both wild‐type and KS‐MR‐KO mice but not in the CCD. Angiotensin II infusion for 3 days has a larger stimulatory effect on ENaC in the DCT2/CNT than in the CCD. Three lines of evidence indicate that angiotensin II can stimulate ENaC by MR‐independent mechanism: (1) angiotensin II perfusion augmented ENaC expression in KS‐MR‐KO mice; (2) angiotensin II stimulated ENaC in the DCT2/CNT but to a lesser degree in the CCD in KS‐MR‐KO mice; (3) angiotensin II infusion augmented benzamil‐induced natriuresis, increased the renal K
+
excretion and corrected hyperkalemia of KS‐MR‐KO mice.

Conclusions
Angiotensin II‐induced stimulation of ENaC occurs mainly in the DCT2/CNT and to a lesser degree in the CCD and MR plays a dominant role in determining ENaC activity in the CCD but to a lesser degree in the DCT2/CNT.
Introduction to “A Conversation between Chinese Artists and
Mexican Painter David Alfaro Siqueiros”
Authors: Cao Jing 
Published: March 2020

Abstract
In October 1956, the Mexican muralist David Siqueiros traveled Beijing and engaged in two dialogues with artists from the Chinese Artists’ Association. His visit came at an inflection point in China’s foreign and cultural policy. As Sino-Soviet relations deteriorated, China used cultural diplomacy to cultivate relationships with unaligned countries in Asia, Africa, and Latin America. China’s cultural policy mirrored this shift by relaxing its adherence to Soviet-style Socialist Realism and promoting new stylistic practices, including a revival of ink painting techniques. This policy shift re-animated a debate among Chinese artists over the best mode of representation for socialist art, with one side arguing that Soviet-style Socialist Realism was the only acceptable style, and the other advocating for the reform of Chinese ink painting techniques. Within this context, Siqueiros’s criticism of Soviet artists and his advice to follow Chinese stylistic traditions set off a rich discussion on new approaches to Socialist Realism within China.
Beyond Evidence
Authors: Schmidt Stiedenroth Kira 
Published: March 2020

Abstract
The therapeutic success of Asian medicine has been discussed mostly in relation to efficacy, effectiveness, and evidence thereof. By taking Unani medicine as an example, this article calls for the reconsideration of the dominance of biomedical frameworks in the anthropological study of Asian medicine by paying closer attention to emic dimensions of successful treatment and their relation to eminence. On the basis of ethnographic fieldwork among practitioners of Unani (Greco-Islamic) medicine in India and following a practice ontology approach, the author examines how private-practicing hakims (Unani physicians) enact successful treatment in their everyday practice. For them, therapeutic success is closely connected to professional authority, a legacy of the Greco-Islamic tradition, in which therapeutic success is also enacted through eminence. Approaching therapeutic success beyond the therapeutic outcome draws attention to further dimensions at stake, revealing that scientific evidence is not necessarily the dominant enactment of successful treatment in Asian medicine.
Assimilation of GOES-R Geostationary Lightning Mapper Flash Extent Density Data in GSI EnKF for the Analysis and Short-Term Forecast of a Mesoscale Convective System
Authors: Kong Rong , Xue Ming , Fierro Alexandre O. , Jung Youngsun , Liu Chengsi , Mansell Edward R. , MacGorman Donald R. 
Published: March 2020

AbstractThe recently launched Geostationary Operational Environmental Satellite “R-series” (GOES-R) satellites carry the Geostationary Lightning Mapper (GLM) that measures from space the total lightning rate in convective storms at high spatial and temporal frequencies. This study assimilates, for the first time, real GLM total lightning data in an ensemble Kalman filter (EnKF) framework. The lightning flash extent density (FED) products at 10-km pixel resolution are assimilated. The capabilities to assimilate GLM FED data are first implemented into the GSI-based EnKF data assimilation (DA) system and tested with a mesoscale convective system (MCS). FED observation operators based on graupel mass or graupel volume are used. The operators are first tuned through sensitivity experiments to determine an optimal multiplying factor to the operator, before being used in FED DA experiments FEDM and FEDV that use the graupel-mass or graupel-volume-based operator, respectively. Their results are compared to a control experiment (CTRL) that does not assimilate any FED data. Overall, both DA experiments outperform CTRL in terms of the analyses and short-term forecasts of FED and composite/3D reflectivity. The assimilation of FED is primarily effective in regions of deep moist convection, which helps improve short-term forecasts of convective threats, including heavy precipitation and lightning. Direct adjustments to graupel mass via observation operator as well as adjustments to other model state variables through flow-dependent ensemble cross covariance within EnKF are shown to work together to generate model-consistent analyses and overall improved forecasts.
The K666N mutation in SF3B1 is associated with increased progression of MDS and distinct RNA splicing
Authors: Dalton W. Brian , Helmenstine Eric , Pieterse Lisa , Li Bing , Gocke Christopher D. , Donaldson Joshua , Xiao Zhijian , Gondek Lukasz P. , Ghiaur Gabriel , Gojo Ivana , Smith B. Douglas , Levis Mark J. , DeZern Amy E. 
Published: March 2020

Key Points
The K666N mutation of SF3B1 has distinct clinicopathologic features in MDS. The K666N mutation of SF3B1 has a distinct RNA splicing profile.
Complex odor control based on ozonation/GAC advanced treatment: optimization and application in one full-scale water treatment plant
Authors: Xia Ping , Zhang Shuangyi , Yu Jianwei , Ye Hui , Zhang Dong , Jiang Lei , Wang Zheng , Yin Daqiang 
Published: March 2020

Abstract
Background
Taste and odor problem in drinking water is one major concern for consumers and water supply. Exploring the odor characteristics and the major odor causing compounds in the source water is the base for odor control in drinking water treatment plant (WTP). In this study, focusing on a newly constructed reservoir with Huangpu River as the source water, the occurrence of typical odorants and their variations were first identified. Correspondingly, the removal behavior in an ozone/GAC advanced treatment process was investigated.

Results
The results indicated that 2-methylisoborneol (2-MIB), geosmin (GSM), and bis (2-chloroisopropyl) ether (BCIE) have major contribution to the musty/earthy and chemical/septic odors in the source water, respectively. Pre-ozonation alone (1 mg L−1) showed limited removal for 2-MIB and BCIE, at less than 30% and 20%, respectively, while combining with coagulation, sedimentation, and sand filtration, the removals were improved to higher than 50%. After post-ozonation, the desired removal was achieved at a 1.5 mg L−1 dosage with all the odorants decreased below the corresponding odor threshold concentrations (OTCs) in the effluents. Furthermore, at a 1 mg L−1 post-ozone addition, by combining with subsequent GAC process, the odor problem was solved as well.

Conclusion
To resolve the odor problem in the drinking water, the concentrations of the odorants at less than their OTCs need to be achieved. As 2-MIB and BCIE have low reactivity towards direct ozonation, a subsequent GAC is needed with a moderate dosage of post-ozonation (1 mg L−1). Thus, for the odor problem in the source water, the suggested operation is: 1 mg L−1 of pre-ozonation in combination with coagulation, sedimentation, and sand filtration, followed by a 1 mg L−1 dosage of post-ozonation and finished by a GAC process.
Cardiac specific transcription factor Csx/Nkx2.5 regulates transient-outward K+ channel expression in pluripotent P19 cell-derived cardiomyocytes
Authors: Uchino Tomoko , Zheng Ming-Qi , Wang Yan , Ono Katsushige 
Published: March 2020

AbstractThe homeobox-containing gene Csx/Nkx2.5 codes several cardiac transcription factors and plays a critical role in early cardiogenesis. We investigated the effect of Csx/Nkx2.5 on the expression of cardiac ion channels using P19-derived cardiomyocytes. P19CL6 cells and P19CL6 cells with Csx/Nkx2.5 overexpression (P19CL6-Csx cells) were induced to differentiate into cardiomyocytes by treatment with dimethyl sulfoxide. Action potentials and membrane currents were measured by whole cell patch clamp at different differentiation stage: the early stage (1–5 days after beating had begun) and the late stage (10–15 days after beating). Expression of Csx/Nkx2.5 mRNA was increased as the differentiation stages advanced in both P19CL6 and P19CL6-Csx cells. In action potential configuration, maximal diastolic potentials in P19CL6-Csx cells exhibited more hyperpolarized potential (‒ 64.2 mV) than those in P19CL6 cells (‒ 54.8 mV,p < 0.01) in the early stage. In P19CL6 cells, among 6 different voltage-gated and ligand-operated K+channels expressed during the early stage, the transient-outward K+channel was most predominant. By overexpression of Csx/Nkx2.5, developmental decrease in the transient-outward K+channel was suppressed. Homeobox-containing gene Csx/Nkx2.5 modifies the amount of distinct ionic channels, during differentiation periods, predominantly changing the expression of the transient-outward K+channel.
Developing a composite outcome measure for frailty prevention trials – rationale, derivation and sample size comparison with other candidate measures
Authors: Witham Miles D. , Wason James , Dodds Richard , Sayer Avan A. 
Published: March 2020

Abstract
Background
Frailty is the loss of ability to withstand a physiological stressor and is associated with multiple adverse outcomes in older people. Trials to prevent or ameliorate frailty are in their infancy. A range of different outcome measures have been proposed, but current measures require either large sample sizes, long follow-up, or do not directly measure the construct of frailty.

Methods
We propose a composite outcome for frailty prevention trials, comprising progression to the frail state, death, or being too unwell to continue in a trial. To determine likely event rates, we used data from the English Longitudinal Study for Ageing, collected 4 years apart. We calculated transition rates between non-frail, prefrail, frail or loss to follow up due to death or illness. We used Markov state transition models to interpolate one- and two-year transition rates and performed sample size calculations for a range of differences in transition rates using simple and composite outcomes.

Results
The frailty category was calculable for 4650 individuals at baseline (2226 non-frail, 1907 prefrail, 517 frail); at follow up, 1282 were non-frail, 1108 were prefrail, 318 were frail and 1936 had dropped out or were unable to complete all tests for frailty. Transition probabilities for those prefrail at baseline, measured at wave 4 were respectively 0.176, 0.286, 0.096 and 0.442 to non-frail, prefrail, frail and dead/dropped out. Interpolated transition probabilities were 0.159, 0.494, 0.113 and 0.234 at two years, and 0.108, 0.688, 0.087 and 0.117 at one year. Required sample sizes for a two-year outcome in a two-arm trial were between 1040 and 7242 for transition from prefrailty to frailty alone, 246 to 1630 for transition to the composite measure, and 76 to 354 using the composite measure with an ordinal logistic regression approach.

Conclusion
Use of a composite outcome for frailty trials offers reduced sample sizes and could ameliorate the effect of high loss to follow up inherent in such trials due to death and illness.
Association between the cytokine storm, immune cell dynamics, and viral replicative capacity in hyperacute HIV infection
Authors: Muema Daniel M. , Akilimali Ngomu A. , Ndumnego Okechukwu C. , Rasehlo Sipho S. , Durgiah Raveshni , Ojwach Doty B.A. , Ismail Nasreen , Dong Mary , Moodley Amber , Dong Krista L. , Ndhlovu Zaza M. , Mabuka Jenniffer M. , Walker Bruce D. , Mann Jaclyn K. , Ndung’u Thumbi 
Published: March 2020

Abstract
Introduction
Immunological damage in acute HIV infection (AHI) may predispose to detrimental clinical sequela. However, studies on the earliest HIV-induced immunological changes are limited, particularly in sub-Saharan Africa. We assessed the plasma cytokines kinetics, and their associations with virological and immunological parameters, in a well-characterized AHI cohort where participants were diagnosed before peak viremia.

Methods
Blood cytokine levels were measured using Luminex and ELISA assays pre-infection, during the hyperacute infection phase (before or at peak viremia, 1–11 days after the first detection of viremia), after peak viremia (24–32 days), and during the early chronic phase (77–263 days). Gag-protease-driven replicative capacities of the transmitted/founder viruses were determined using a green fluorescent reporter T cell assay. Complete blood counts were determined before and immediately following AHI detection before ART initiation.

Results
Untreated AHI was associated with a cytokine storm of 12 out of the 33 cytokines analyzed. Initiation of ART during Fiebig stages I–II abrogated the cytokine storm. In untreated AHI, virus replicative capacity correlated positively with IP-10 (rho = 0.84, P < 0.001) and IFN-alpha (rho = 0.59, P = 0.045) and inversely with nadir CD4+ T cell counts (rho = − 0.58, P = 0.048). Hyperacute HIV infection before the initiation of ART was associated with a transient increase in monocytes (P < 0.001), decreased lymphocytes (P = 0.011) and eosinophils (P = 0.003) at Fiebig stages I–II, and decreased eosinophils (P < 0.001) and basophils (P = 0.007) at Fiebig stages III–V. Levels of CXCL13 during the untreated hyperacute phase correlated inversely with blood eosinophils (rho = − 0.89, P < 0.001), basophils (rho = − 0.87, P = 0.001) and lymphocytes (rho = − 0.81, P = 0.005), suggesting their trafficking into tissues. In early treated individuals, time to viral load suppression correlated positively with plasma CXCL13 at the early chronic phase (rho = 0.83, P = 0.042).

Conclusion
While commencement of ART during Fiebig stages I–II of AHI abrogated the HIV-induced cytokine storm, significant depletions of eosinophils, basophils, and lymphocytes, as well as transient expansions of monocytes, were still observed in these individuals in the hyperacute phase before the initiation of ART, suggesting that even ART initiated during the onset of viremia does not abrogate all HIV-induced immune changes.
Light-enhanced cognitive behavioural therapy for sleep and fatigue: study protocol for a randomised controlled trial during chemotherapy for breast cancer
Authors: Bean Helena R. , Stafford Lesley , Little Ruth , Diggens Justine , Ftanou Maria , Alexander Marliese , Francis Prudence A. , Bei Bei , Wiley Joshua F. 
Published: March 2020

Abstract
Background
Women with breast cancer experience a significantly higher prevalence of sleep disturbance and insomnia than the general population. The experience of persistent sleep disturbance places these women at a higher risk of psychological and physical morbidity and a reduced quality of life. Treatment for sleep in this population is not part of routine care and is often managed inadequately. This randomised controlled trial will examine the combined effects of cognitive behavioural therapy (CBT) and bright light therapy (BLT) on the symptoms of insomnia, fatigue and mental health.

Method/design
Women diagnosed with breast cancer who receive intravenous chemotherapy treatment at a quaternary referral metropolitan cancer centre in Melbourne, Australia, will be recruited. Recruitment will occur after diagnosis and prior to completion of chemotherapy. Eligible women will be randomised to the combined CBT and BLT intervention (CBT+) or relaxation audio-enhanced treatment as usual (TAU+). The CBT+ group will receive one face-to-face session on sleep strategies, one subsequent telephone call, and seven email packages containing CBT-based information and strategies. CBT+ participants will also wear Luminette® light glasses for 20 min each morning for the 6-week duration of the intervention. Women in TAU+ will receive two relaxation audio tracks via email. Outcomes will be measured at multiple points throughout the 6 weeks. Primary outcomes will be symptoms of insomnia and sleep efficiency, measured using the Insomnia Severity Index and a self-reported sleep diary. Secondary outcomes include objective measures of sleep assessed using the ActiGraph wGT3X-BT, and sleep-related complaints, fatigue and mental health, all assessed using the Patient-Reported Outcomes Measurement Information System (PROMIS). Data will also be collected on potential treatment moderators and mechanisms and adherence to treatment. There will be 3-month follow-up measurements of insomnia symptoms, fatigue, sleep-related impairment, sleep disturbance, depression and anxiety.

Discussion
This is the first randomised controlled trial to combine CBT and BLT for the treatment of sleep disturbance in women with breast cancer. This novel design addresses the multiple causal factors for sleep complaints in this population. Results from this trial will advance knowledge in this field and may have important clinical implications for how best to treat sleep disturbance and insomnia in this population. If effective, the largely email-based format of the intervention would allow for relatively easy translation.

Trial Registration
Australian New Zealand Clinical Trials Registry (ANZCTR), ACTRN12618001255279. Retrospectively registered on 25 July 2018.
Patient satisfaction with primary care physician performance in a multicultural population
Authors: Hayek Samah , Derhy Shany , Smith Mathew Lee , Towne Samuel D. , Zelber-Sagi Shira 
Published: March 2020

Abstract
Background
A key component of the quality of health care is patient satisfaction, particularly in regard to Primary Care Physician (PCP), which represents the first contact with health care services. Patient satisfaction is associated with ethnic, regional and socio-demographic differences, due to differences in service quality, patient-doctor communication, and the patient’s perceptions. The aim of this study was to evaluate patients’ satisfaction related to primary care physicians’ (PCP) performance and to explore potential differences by ethnicity in a multicultural population.

Methods
A national cross-sectional telephone survey was conducted, among a random sample of the Israeli population aged ≥25 years. Satisfaction level from performance of PCP was assessed using a validated questionnaire (30 items; 6 different domains).

Results
The final sample included (n = 827 Jews; n = 605 Arabs, mean age 54.7(±14.9). In the adjusted logistic regression models, Arabs reported lower general satisfaction related to PCPs’ performance as compared to Jews (adjusted odds ratio (AOR), 0.63; (95% CI: 0.40–0.98). Arabs reported lower satisfaction related to PCPs’ performance across the following domains: communication skills (AOR, 0.42; 95% CI, 0.22–0.82); interpersonal manners (AOR, 0.37; 95% CI, 0.24–0.58); and time spent with the patients (AOR, 0.60; 95% CI, 0.43–0.85).

Conclusions
Jews and Arabs were very satisfied with PCPs’ performance. However, there are ethnic differences in the extent of satisfaction level related to the performance of PCP. Satisfaction from PCPs’ performance may be achieved by improving the communication skills of the PCP, encouraging interpersonal interaction between the PCP and the patient, and devoting more time to the patient during the visits.