bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cysteine 202 of cyclophilin D is a site of multiple post-translational modifications and plays a role in cardioprotection
Authors: Amanakis Georgios , Sun Junhui , Fergusson Maria M , McGinty Shane , Liu Chengyu , Molkentin Jeffery D , Murphy Elizabeth 
Published: February 2020

Abstract

Aims
Cyclophilin-D is a well-known regulator of the mitochondrial permeability transition pore (PTP), the main effector of cardiac ischaemia/reperfusion injury. However, the binding of CypD to the PTP is poorly understood. Cysteine 202 (C202) of CypD is highly conserved among species and can undergo redox-sensitive post-translational modifications. We investigated whether C202 regulates the opening of PTP.

Methods and results
We developed a knock-in mouse model using CRISPR where CypD-C202 was mutated to a serine (C202S). Infarct size is reduced in CypD-C202S Langendorff perfused hearts compared to wild type (WT). Cardiac mitochondria from CypD-C202S mice also have higher calcium retention capacity compared to WT. Therefore, we hypothesized that oxidation of C202 might target CypD to the PTP. Indeed, isolated cardiac mitochondria subjected to oxidative stress exhibit less binding of CypD-C202S to the proposed PTP component F1F0-ATP-synthase. We previously found C202 to be S-nitrosylated in ischaemic preconditioning. Cysteine residues can also undergo S-acylation, and C202 matched an S-acylation motif. S-acylation of CypD-C202 was assessed using a resin-assisted capture (Acyl-RAC). WT hearts are abundantly S-acylated on CypD C202 under baseline conditions indicating that S-acylation on C202 per se does not lead to PTP opening. CypD C202S knock-in hearts are protected from ischaemia/reperfusion injury suggesting further that lack of CypD S-acylation at C202 is not detrimental (when C is mutated to S) and does not induce PTP opening. However, we find that ischaemia leads to de-acylation of C202 and that calcium overload in isolated mitochondria promotes de-acylation of CypD. Furthermore, a high bolus of calcium in WT cardiac mitochondria displaces CypD from its physiological binding partners and possibly renders it available for interaction with the PTP.

Conclusions
Taken together the data suggest that with ischaemia CypD is de-acylated at C202 allowing the free cysteine residue to undergo oxidation during the first minutes of reperfusion which in turn targets it to the PTP.
Changing beliefs on English: study abroad for teacher development
Authors: Mayumi Kayoko , Hüttner Julia 
Published: February 2020

Abstract
Many teachers of English aspire to study in anglophone countries, especially at postgraduate level, as a means to support their own professional development. While the overt interest lies in acquiring cutting-edge theories in ELT to improve their classroom practices, a sojourn abroad is also valued as a cultural and linguistic immersion in the English language. Within the vibrant research scene of study abroad, comparatively little is known about the effect of studying abroad on older participants. This study addresses mature teachers as students abroad and focuses on the challenges and changes to their existing professional beliefs triggered by their foreign academic sojourn. The participants are four Japanese teachers of English who attended MA programmes in the UK. The analysis of a corpus of 2046 minutes of in-depth interviews, conducted over a period of 20 months, revealed how some core professional beliefs on English were reformulated, and triggered teacher development.
Lithospheric structure of Hubei Province, central China, from Rayleigh wave tomography: insight into the spatial contact relationship between the Yangtze Plate and the eastern Qinling-Dabie orogenic belt
Authors: Wu Tengfei , Zhang Shuangxi , Cao Zijun , Li Mengkui , Hua Yujin , Fu Xiaoying , Wei Yu 
Published: February 2020

SUMMARYKnowledge about the spatial contact relationship between the Yangtze Plate and the eastern Qinling-Dabie orogenic belt can not only provide a scientific basis for the exploration of mineral resources, disaster prevention and earthquake prediction in the region, but also play an important role in reconstructing the geological process of the central orogenic belt. Hence, high-resolution lithospheric velocity model is essential to address these geological problems. In this study, using waveform data from 48 stations in Hubei Province and adjacent regions, central China, we invert for a 3-D S-wave velocity structure model of the crust and upper mantle from Rayleigh wave tomography. Our model reveals the complex subduction pattern of the Yangtze Plate to the north and the thrust-nappe tectonics of the Qinling-Dabie orogenic belt along the Mianlue suture with different scales and different deformation strengths. In addition, in the central part of Hubei Province, the local Yangtze slab has been broken into several pieces, among which the upwelling low-velocity anomalies appear. Moreover, the southern margin of the Dabie orogenic belt has undergone thrusting-nappe movement, and a series of associated structures are formed in the northern margin of the middle Yangtze platform. The contact zone between the two blocks in this area is composed of a series of thrust faults with dextrorotation slip component. Finally, based on the 3-D S-wave velocity image of Hubei Province and its vertical cross-section profiles along three different directions, three dynamic models are proposed to explain the spatial contact relationship between the Yangtze Plate and the eastern Qinling-Dabie orogenic belt in different regions.
Surgical treatment of pulmonary sequestration in adults and children: long-term results
Authors: Trabalza Marinucci Beatrice , Maurizi Giulio , Vanni Camilla , Cardillo Giuseppe , Poggi Camilla , Pardi Valerio , Inserra Alessandro , Rendina Erino A 
Published: February 2020

Abstract

OBJECTIVES
Few experiences comparing paediatric and adult patients treated for pulmonary sequestration (PS) have been reported. Surgical treatment is considered the best choice, but the time of surgery is still controversial. We present our experience in this setting, comparing characteristics, histological results and outcome of paediatric and adult patients undergoing PS resection.

METHODS
Between 1998 and 2017, a total of 74 patients underwent lobectomy or sublobar resection for PS. Sixty patients were children (group A: ≤16 years old) and 14 were adults (group B: &gt;16 years old). Preoperative diagnosis was radiological. PS was intralobar (42 cases) and extralobar (32 cases). The operation was a muscle-sparing lateral thoracotomy or video-assisted thoracoscopic surgery. Preoperative characteristics, histological results and short-/long-term results of the 2 groups were retrospectively analysed and compared.

RESULTS
Thirty-seven percent of the patients in group A presented with respiratory symptoms and 79% in group B (P = 0.44). Most symptomatic patients were treated with a lobectomy. In group A, 2 patients (3%) had a malignant transformation of the lesion. Patients with a prenatal diagnosis treated after the age of 1 year became more symptomatic than those operated on before the age of 1 year (57% vs 23%; P = 0.08). No differences were found in postoperative complications. Long-term stable remission of respiratory symptoms was obtained in 91% of patients in group A and 100% in group B. Adulthood (P = 0.03) and the association with congenital cystic adenomatoid malformation (P = 0.03) were negative prognostic factors for the development of respiratory symptoms.

CONCLUSIONS
Surgical treatment of PS is safe and feasible. Despite the small number of patients included, study results indicated that an early operation during childhood may prevent the subsequent development of respiratory symptoms. Surgical treatment is also recommended to prevent the rare transformation into malignancy.
Laryngotracheal Reconstruction in the Pediatric Burn Patient: Surgical Techniques and Decision Making
Authors: Jayawardena Asitha D L , Bouhabel Sarah , Sheridan Robert L , Hartnick Christopher J 
Published: February 2020

AbstractThe management of laryngotracheal stenosis (LTS) in the pediatric burn patient is complex and requires a multidisciplinary approach. The mainstay of treatment for LTS is laryngotracheal reconstruction (LTR), however, limited reports of burn-specific LTR techniques exist. Here, we provide insight into the initial airway evaluation, surgical decision making, anesthetic challenges, and incision modifications based on our experience in treating patients with this pathology. The initial airway evaluation can be complicated by microstomia, trismus, and neck contractures—the authors recommend treatment of these complications prior to initial airway evaluation to optimize safety. The surgical decision making regarding pursuing single-stage LTR, double-stage LTR, and 1.5-stage LTR can be challenging—the authors recommend 1.5-stage LTR when possible due to the extra safety of rescue tracheostomy and the decreased risk of granuloma, which is especially important in pro-inflammatory burn physiology. Anesthetic challenges include obtaining intravenous access, securing the airway, and intravenous induction—the authors recommend peripherally inserted central catheter when appropriate, utilizing information from the initial airway evaluation to secure the airway, and avoidance of succinylcholine upon induction. Neck and chest incisions are often within the TBSA covered by the burn injury—the authors recommend modifying typical incisions to cover unaffected skin whenever possible in order to limit infection and prevent wound healing complications. Pediatric LTR in the burn patient is challenging, but can be safe when the surgeon is thoughtful in their decision making.
Use of Mechanical Stretching to Treat Skin Graft Contracture
Authors: Zhou Jinfeng , Zhao Youcai , Yang Wengbo , Du Qianming , Yin Jun , Gu Yanqing , Hao Chao 
Published: February 2020

AbstractAfter transplantation, skin grafts contract to different degrees, thus affecting the appearance and function of the skin graft sites. The exact mechanism of contracture after skin grafting remains unclear, and reliable treatment measures are lacking; therefore, new treatment methods must be identified. Many types of centripetal contraction forces affect skin graft operation, thus leading to centripetal contracture. Therefore, antagonizing the centripetal contraction of skin grafts may be a feasible method to intervene in skin contracture. Here, the authors propose the first reported mechanical stretching method to address contracture after skin grafting. A full-thickness skin graft model was established on the backs of SD rats. The skin in the experimental group was stretched unilaterally or bidirectionally with a self-made elastic stretching device, whereas the skin was non-stretched in the control group. The rats were sacrificed 2 weeks after stretching. The area, length, and width of the skin were measured. The grafts were cut and fixed with formalin. Routine paraffin sections were stained with hematoxylin-eosin, picric acid-Sirius red, Victoria blue, and anti-alpha-smooth muscle actin (SMA). Mechanical stretching made the graft lengthen in the direction of the stress and had an important influence on collagen deposition and alpha-SMA expression in the graft. This method warrants further in-depth study to provide a basis for clinical application.
Movement of Rhagoletis mendax (Diptera: Tephritidae) in Fruit-Bearing Wild Blueberry Fields. Part II
Authors: Drummond Francis A , Collins Judith A , Bushmann Sara L 
Published: February 2020

Abstract
During dispersal into fruit-bearing wild blueberry fields, blueberry maggot flies were highly active during all daylight hours as revealed by trap captures, although in one trial afternoon activity was greater than morning activity. Flies were not captured in traps at night, although observations in growth chambers showed that their activity at night, measured as displacement of position, was equal to daylight conditions. Flies were shown to fly at low altitude, just above the crop canopy, and screen fencing was shown to be effective at reducing colonization of plots, presumably due to their low height during flight. Over a 4-yr mark–capture study, colonization rate was shown to be low at 9.7 m/d, although a separate 2010 study showed higher rates at 14.1 and 28.0 m/d. Movement was shown to be nondirectional or random in the field, but a constrained random walk exhibiting direction into the field. Weed cover and high fruit density were associated with higher fly relative abundance, suggesting these field characteristics served as attractors slowing colonization rate into a field. Transect trap studies showed the temporal and spatial pattern of fly colonization into commercial wild blueberry fields, one of a slow wave that penetrates into the field interior as the season progresses. There is also an increase in fly abundance within-field edges and adjacent forest. The ‘stacking’ of flies along a field edge and slow movement rate into a field was shown through simulation to be a result of nondirectional short-distance dispersal of flies.
Biting Behavior and Molecular Identification of Aedes aegypti (Diptera: Culicidae) Subspecies in Some Selected Recent Yellow Fever Outbreak Communities in Northern Ghana
Authors: Captain-Esoah Millicent , Kweku Baidoo Philip , Frempong Kwadwo K , Adabie-Gomez Delphina , Chabi Joseph , Obuobi Dorothy , Kwame Amlalo Godwin , Balungnaa Veriegh Francis , Donkor Martin , Asoala Victor , Behene Eric , Adjei Boakye Daniel , Dadzie Samuel Kweku 
Published: February 2020

AbstractAedes aegypti (L.) (Diptera: Culicidae) is a diurnal feeder that lives in close association with human populations. It is the principal vector of yellow fever, dengue fever and the Zika Virus. Issues of arboviral diseases have been on the ascendency in most countries including Ghana where Aedes mosquito is the main vector of yellow fever. A comparative study of the biting behavior of Ae. aegypti and the identification of subspecies were undertaken using molecular technique. Standard human landing technique was used to collect both indoor and outdoor biting mosquitoes at three zones located in the Upper East (Bolgatanga), Upper West (Nadowli), and Northern (Damongo) Regions of Ghana during the dry and rainy seasons between 0600 and 1800 Greenwich Mean Time (GMT). All collected mosquitoes were identified morphologically using taxonomic keys. random amplified polymorphic DNA polymerase chain reaction was used to categorize Ae. aegypti into subspecies. Adult female Aedes mosquitoes identified formed 62% (n = 1,206) of all female mosquitoes collected. Aedes aegypti 98% and Aedes vittatus 2% were the only Aedes species identified. Bolgatanga recorded the largest number of Ae. aegypti 42%, whereas Nadowli 22% recorded the least. Aedes vittatus was observed in Nadowli. Aedes aegypti exhibited a bimodal biting behavior peaking at 0600–0800 GMT and 1500–1600 h GMT. Molecular findings revealed 69% Ae. aegypti aegypti and 31% Ae. aegypti formosus as the two subspecies (n = 110). This information is important for implementing effective vector control programs in the three regions of the northern Ghana.
Severe traumatic brain injury
Authors: Newcombe Virginia , Cooper Jamie 
Published: March 2020

Abstract
This chapter is centred on a case study on traumatic brain injury. This topic is one of the key challenging areas in critical care medicine and one that all intensive care staff will encounter. The chapter is based on a detailed case history, ensuring clinical relevance, together with relevant images (e.g. chest X-rays and computed tomography scans), making this easily relatable to daily practice in the critical care unit. The chapter is punctuated by evidence-based, up-to-date learning points, which highlight key information for the reader. Throughout the chapter, a topic expert provides contextual advice and commentary, adding practical expertise to the standard textbook approach and reinforcing key messages.
Post-cardiac arrest care and prognostication
Authors: Barnett Justine , Nolan Jerry 
Published: March 2020

Abstract
This chapter is centred on a case study on brain injury after cardiac arrest. This topic is one of the key challenging areas in critical care medicine and one that all intensive care staff will encounter. The chapter is based on a detailed case history, ensuring clinical relevance, together with relevant images, making this easily relatable to daily practice in the critical care unit. The chapter is punctuated by evidence-based, up-to-date learning points, which highlight key information for the reader. Throughout the chapter, a topic expert provides contextual advice and commentary, adding practical expertise to the standard textbook approach and reinforcing key messages.
Subarachnoid haemorrhage
Authors: Kirkman Matthew A. , Smith Martin 
Published: March 2020

Abstract
This chapter is centred on a case study on subarachnoid haemorrhage. This topic is one of the key challenging areas in critical care medicine and one that all intensive care staff will encounter. The chapter is based on a detailed case history, ensuring clinical relevance, together with relevant images, making this easily relatable to daily practice in the critical care unit. The chapter is punctuated by evidence-based, up-to-date learning points, which highlight key information for the reader. Throughout the chapter, a topic expert provides contextual advice and commentary, adding practical expertise to the standard textbook approach and reinforcing key messages.
Acute-on-chronic respiratory failure
Authors: Hunt Richard , MacNaughton Peter 
Published: March 2020

Abstract
This chapter is centred on a case study on acute-on-chronic respiratory failure. This topic is one of the key challenging areas in critical care medicine and one that all intensive care staff will encounter. The chapter is based on a detailed case history, ensuring clinical relevance, together with relevant images, making this easily relatable to daily practice in the critical care unit. The chapter is punctuated by evidence-based, up-to-date learning points, which highlight key information for the reader. Throughout the chapter, a topic expert provides contextual advice and commentary, adding practical expertise to the standard textbook approach and reinforcing key messages.
Feeding, access, and thromboprophylaxis
Authors: Huntley Martin , Moonesinghe Ramani 
Published: March 2020

Abstract
This chapter is centred on a case study on feeding, access and thromboprophylaxis. This topic is one of the key challenging areas in critical care medicine and one that all intensive care staff will encounter. The chapter is based on a detailed case history, ensuring clinical relevance, together with relevant images, making this easily relatable to daily practice in the critical care unit. The chapter is punctuated by evidence-based, up-to-date learning points, which highlight key information for the reader. Throughout the chapter, a topic expert provides contextual advice and commentary, adding practical expertise to the standard textbook approach and reinforcing key messages.
Malignancy and critical illness
Authors: Desai Nishita , Wares Gary 
Published: March 2020

Abstract
This chapter is centred on a case study on malignancy. This topic is one of the key challenging areas in critical care medicine and one that all intensive care staff will encounter. The chapter is based on a detailed case history, ensuring clinical relevance, together with relevant images, making this easily relatable to daily practice in the critical care unit. The chapter is punctuated by evidence-based, up-to-date learning points, which highlight key information for the reader. Throughout the chapter, a topic expert provides contextual advice and commentary, adding practical expertise to the standard textbook approach and reinforcing key messages.
Major burns
Authors: Moxham Sian Alys , Young Amber E. 
Published: March 2020

Abstract
This chapter is centred on a case study on burns. This topic is one of the key challenging areas in critical care medicine and one that all intensive care staff will encounter. The chapter is based on a detailed case history, ensuring clinical relevance, together with relevant images, making this easily relatable to daily practice in the critical care unit. The chapter is punctuated by evidence-based, up-to-date learning points, which highlight key information for the reader. Throughout the chapter, a topic expert provides contextual advice and commentary, adding practical expertise to the standard textbook approach and reinforcing key messages.
Stars made in outflows may populate the stellar halo of the Milky Way
Authors: Yu Sijie , Bullock James S , Wetzel Andrew , Sanderson Robyn E , Graus Andrew S , Boylan-Kolchin Michael , Nierenberg Anna M , Grudić Michael Y , Hopkins Philip F , Kereš Dušan , Faucher-Giguère Claude-André 
Published: February 2020

ABSTRACT
We study stellar-halo formation using six Milky-Way-mass galaxies in FIRE-2 cosmological zoom simulations. We find that $5{-}40{{\ \rm per\ cent}}$ of the outer (50–300 kpc) stellar halo in each system consists of in-situ stars that were born in outflows from the main galaxy. Outflow stars originate from gas accelerated by superbubble winds, which can be compressed, cool, and form co-moving stars. The majority of these stars remain bound to the halo and fall back with orbital properties similar to the rest of the stellar halo at z = 0. In the outer halo, outflow stars are more spatially homogeneous, metal-rich, and alpha-element-enhanced than the accreted stellar halo. At the solar location, up to $\sim \!10 {{\ \rm per\ cent}}$ of our kinematically identified halo stars were born in outflows; the fraction rises to as high as $\sim \!40{{\ \rm per\ cent}}$ for the most metal-rich local halo stars ([Fe/H] &gt;−0.5). Such stars can be retrograde and create features similar to the recently discovered Milky Way ‘Splash’ in phase space. We conclude that the Milky Way stellar halo could contain local counterparts to stars that are observed to form in molecular outflows in distant galaxies. Searches for such a population may provide a new, near-field approach to constraining feedback and outflow physics. A stellar halo contribution from outflows is a phase-reversal of the classic halo formation scenario of Eggen, Lynden-Bell & Sandange, who suggested that halo stars formed in rapidly infalling gas clouds. Stellar outflows may be observable in direct imaging of external galaxies and could provide a source for metal-rich, extreme-velocity stars in the Milky Way.
The spin measurement of the black hole in 4U 1543-47 constrained with the X-ray reflected emission
Authors: Dong Yanting , García Javier A , Steiner James F , Gou Lijun 
Published: February 2020

ABSTRACT
4U 1543-47 is a low-mass X-ray binary that harbours a stellar-mass black hole located in our Milky Way galaxy. In this paper, we revisit seven data sets that were in the Steep Power Law state of the 2002 outburst. The spectra were observed by the Rossi X-ray Timing Explorer. We have carefully modelled the X-ray reflection spectra and made a joint-fit to these spectra with relxill for the reflected emission. We found a moderate black hole spin, which is $0.67_{-0.08}^{+0.15}$ at 90 per cent statistical confidence. Negative and low spins (&lt;0.5) at more than 99 per cent statistical confidence are ruled out. In addition, our results indicate that the model requires a supersolar iron abundance: $5.05_{-0.26}^{+1.21}$, and the inclination angle of the inner disc is $36.3_{-3.4}^{+5.3}$ deg. This inclination angle is appreciably larger than the binary orbital inclination angle (∼21 deg); this difference is possibly a systematic artefact of the artificially low density employed in the reflection model for this X-ray binary system.