bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mild electrical stimulation with heat shock attenuates renal pathology in adriamycin-induced nephrotic syndrome mouse model
Authors: Teramoto Keisuke , Tsurekawa Yu , Suico Mary Ann , Kaseda Shota , Omachi Kohei , Yokota Tsubasa , Kamura Misato , Piruzyan Mariam , Kondo Tatsuya , Shuto Tsuyoshi , Araki Eiichi , Kai Hirofumi 
Published: October 2020

AbstractNephrotic syndrome (NS) is a renal disorder that is characterized by massive proteinuria, hypoalbuminemia and edema. One of the main causes of NS is focal segmental glomerulosclerosis (FSGS), which has extremely poor prognosis. Although steroids and immunosuppressants are the first line of treatment, some FSGS cases are refractory, prompting the need to find new therapeutic strategies. We have previously demonstrated that an optimized combination treatment of mild electrical stimulation (MES) and heat shock (HS) has several biological benefits including the amelioration of the pathologies of the genetic renal disorder Alport syndrome. Here, we investigated the effect of MES + HS on adriamycin (ADR)-induced NS mouse model. MES + HS suppressed proteinuria and glomerulosclerosis induced by ADR. The expressions of pro-inflammatory cytokines and pro-fibrotic genes were also significantly downregulated by MES + HS. MES + HS decreased the expression level of cleaved caspase-3 and the number of TUNEL-positive cells, indicating that MES + HS exerted anti-apoptotic effect. Moreover, MES + HS activated the Akt signaling and induced the phosphorylation and inhibition of the apoptotic molecule BAD. In in vitro experiment, the Akt inhibitor abolished the MES + HS-induced Akt-BAD signaling and anti-apoptotic effect in ADR-treated cells. Collectively, our study suggested that MES + HS modulates ADR-induced pathologies and has renoprotective effect against ADR-induced NS via regulation of Akt-BAD axis.
Machine learning prediction for mortality of patients diagnosed with COVID-19: a nationwide Korean cohort study
Authors: An Chansik , Lim Hyunsun , Kim Dong-Wook , Chang Jung Hyun , Choi Yoon Jung , Kim Seong Woo 
Published: October 2020

AbstractThe rapid spread of COVID-19 has resulted in the shortage of medical resources, which necessitates accurate prognosis prediction to triage patients effectively. This study used the nationwide cohort of South Korea to develop a machine learning model to predict prognosis based on sociodemographic and medical information. Of 10,237 COVID-19 patients, 228 (2.2%) died, 7772 (75.9%) recovered, and 2237 (21.9%) were still in isolation or being treated at the last follow-up (April 16, 2020). The Cox proportional hazards regression analysis revealed that age > 70, male sex, moderate or severe disability, the presence of symptoms, nursing home residence, and comorbidities of diabetes mellitus (DM), chronic lung disease, or asthma were significantly associated with increased risk of mortality (p ≤ 0.047). For machine learning, the least absolute shrinkage and selection operator (LASSO), linear support vector machine (SVM), SVM with radial basis function kernel, random forest (RF), and k-nearest neighbors were tested. In prediction of mortality, LASSO and linear SVM demonstrated high sensitivities (90.7% [95% confidence interval: 83.3, 97.3] and 92.0% [85.9, 98.1], respectively) and specificities (91.4% [90.3, 92.5] and 91.8%, [90.7, 92.9], respectively) while maintaining high specificities > 90%, as well as high area under the receiver operating characteristics curves (0.963 [0.946, 0.979] and 0.962 [0.945, 0.979], respectively). The most significant predictors for LASSO included old age and preexisting DM or cancer; for RF they were old age, infection route (cluster infection or infection from personal contact), and underlying hypertension. The proposed prediction model may be helpful for the quick triage of patients without having to wait for the results of additional tests such as laboratory or radiologic studies, during a pandemic when limited medical resources must be wisely allocated without hesitation.
Nearshore neonate dispersal of Atlantic leatherback turtles (Dermochelys coriacea) from a non-recovering subpopulation
Authors: Hoover Aimee L. , Shillinger George L. , Williamson Sean A. , Reina Richard D. , Bailey Helen 
Published: October 2020

AbstractThe cryptic ‘lost years’ of sea turtles challenge conservation efforts due to unknown movements and habitat utilisation of young life stages. Behavioural information strengthens dispersal and habitat utilisation models estimating unidentified movements. In this study, leatherback hatchlings were actively tracked with miniature acoustic tags off the east coast of Costa Rica for 83.15 min (± 9.12 SD) to determine their movements and swimming behaviour. Drifters were deployed throughout the tracking process to obtain surface current data. Hatchling (n = 42) over-ground and in-water swimming speed and bearing were calculated. Mean over-ground distance travelled was 2.03 km (± 0.71 km SD) with an over-ground average swim speed of 0.41 m/s (± 0.15 m/s SD). Mean bearing was 108.08° (± 20.19° SD) compared to the 137.56° (± 44.00° SD) bearing of nearshore ocean currents during tracking. Hatchling mean in-water swimming speed was 0.25 m/s (± 0.09 m/s SD). The lower in-water speed suggests hatchlings were advected by the currents, with overall movement strongly influenced by the current direction. This information can be assimilated into broader spatiotemporal distribution models to interpret the influence of directional swimming on ecosystem utilisation and help to achieve informed management decisions across all life stages of the population.
Molecular specializations of deep cortical layer analogs in songbirds
Authors: Nevue Alexander A. , Lovell Peter V. , Wirthlin Morgan , Mello Claudio V. 
Published: October 2020

AbstractHow the evolution of complex behavioral traits is associated with the emergence of novel brain pathways is largely unknown. Songbirds, like humans, learn vocalizations via tutor imitation and possess a specialized brain circuitry to support this behavior. In a comprehensive in situ hybridization effort, we show that the zebra finch vocal robust nucleus of the arcopallium (RA) shares numerous markers (e.g. SNCA, PVALB) with the adjacent dorsal intermediate arcopallium (AId), an avian analog of mammalian deep cortical layers with involvement in motor function. We also identify markers truly unique to RA and thus likely linked to modulation of vocal motor function (e.g. KCNC1, GABRE), including a subset of the known shared markers between RA and human laryngeal motor cortex (e.g. SLIT1, RTN4R, LINGO1, PLXNC1). The data provide novel insights into molecular features unique to vocal learning circuits, and lend support for the motor theory for vocal learning origin.
Plasmopara viticola infection affects mineral elements allocation and distribution in Vitis vinifera leaves
Authors: Cesco Stefano , Tolotti Anna , Nadalini Stefano , Rizzi Stefano , Valentinuzzi Fabio , Mimmo Tanja , Porfido Carlo , Allegretta Ignazio , Giovannini Oscar , Perazzolli Michele , Cipriani Guido , Terzano Roberto , Pertot Ilaria , Pii Youry 
Published: October 2020

AbstractPlasmopara viticolais one of the most important pathogens infectingVitis viniferaplants. The interactions amongP. viticolaand both susceptible and resistant grapevine plants have been extensively characterised, at transcriptomic, proteomic and metabolomic levels. However, the involvement of plants ionome in the response against the pathogen has been completely neglected so far. Therefore, this study was aimed at investigating the possible role of leaf ionomic modulation during compatible and incompatible interactions betweenP. viticolaand grapevine plants. In susceptible cultivars, a dramatic redistribution of mineral elements has been observed, thus uncovering a possible role for mineral nutrients in the response against pathogens. On the contrary, the resistant cultivars did not present substantial rearrangement of mineral elements at leaf level, except for manganese (Mn) and iron (Fe). This might demonstrate that, resistant cultivars, albeit expressing the resistance gene, still exploit a pathogen response mechanism based on the local increase in the concentration of microelements, which are involved in the synthesis of secondary metabolites and reactive oxygen species. Moreover, these data also highlight the link between the mineral nutrition and plants’ response to pathogens, further stressing that appropriate fertilization strategies can be fundamental for the expression of response mechanisms against pathogens.
Retrograde signaling by a mtDNA-encoded non-coding RNA preserves mitochondrial bioenergetics
Authors: Blumental-Perry A. , Jobava R. , Bederman I. , Degar A. J. , Kenche H. , Guan B. J. , Pandit K. , Perry N. A. , Molyneaux N. D. , Wu J. , Prendergas E. , Ye Z.-W. , Zhang J. , Nelson C. E. , Ahangari F. , Krokowski D. , Guttentag S. H. , Linden P. A. , Townsend D. M. , Miron A. , Kang M.-J. , Kaminski N. , Perry Y. , Hatzoglou M. 
Published: October 2020

AbstractAlveolar epithelial type II (AETII) cells are important for lung epithelium maintenance and function. We demonstrate that AETII cells from mouse lungs exposed to cigarette smoke (CS) increase the levels of the mitochondria-encoded non-coding RNA, mito-RNA-805, generated by the control region of the mitochondrial genome. The protective effects of mito-ncR-805 are associated with positive regulation of mitochondrial energy metabolism, and respiration. Levels of mito-ncR-805 do not relate to steady-state transcription or replication of the mitochondrial genome. Instead, CS-exposure causes the redistribution of mito-ncR-805 from mitochondria to the nucleus, which correlated with the increased expression of nuclear-encoded genes involved in mitochondrial function. These studies reveal an unrecognized mitochondria stress associated retrograde signaling, and put forward the idea that mito-ncRNA-805 represents a subtype of small non coding RNAs that are regulated in a tissue- or cell-type specific manner to protect cells under physiological stress.
Cellular-resolution mapping uncovers spatial adaptive filtering at the rat cerebellum input stage
Authors: Casali Stefano , Tognolina Marialuisa , Gandolfi Daniela , Mapelli Jonathan , D’Angelo Egidio 
Published: October 2020

AbstractLong-term synaptic plasticity is thought to provide the substrate for adaptive computation in brain circuits but very little is known about its spatiotemporal organization. Here, we combined multi-spot two-photon laser microscopy in rat cerebellar slices with realistic modeling to map the distribution of plasticity in multi-neuronal units of the cerebellar granular layer. The units, composed by ~300 neurons activated by ~50 mossy fiber glomeruli, showed long-term potentiation concentrated in the core and long-term depression in the periphery. This plasticity was effectively accounted for by an NMDA receptor and calcium-dependent induction rule and was regulated by the inhibitory Golgi cell loops. Long-term synaptic plasticity created effective spatial filters tuning the time-delay and gain of spike retransmission at the cerebellum input stage and provided a plausible basis for the spatiotemporal recoding of input spike patterns anticipated by the motor learning theory.
Aglycosylated antibody-producing mice for aglycosylated antibody-lectin coupled immunoassay for the quantification of tumor markers (ALIQUAT)
Authors: Lee Nan-Ee , Kim Sun Hee , Yu Dae-Yeul , Woo Eui-Jeon , Kim Myung-Il , Seong Gi-Sang , Lee Sun Min , Ko Jeong-Heon , Kim Yong-Sam 
Published: October 2020

AbstractTargeting aberrant glycoforms has been validated for in vitro cancer diagnostic development, and several assays are currently in routine clinical use. Because N-glycans in Fc region of antibodies show cross-reactivity with various lectins, high-quality aglycosylated antibodies are exceptionally important for immunoassay platform-based quantitative measurements. Previously, aglycosylated antibody acquisition relied on incomplete, uneconomical and onerous enzymatic and chemical methods. Here, we edited four murine immunoglobulin G genes using adenine base-editing and homology-directed recombination (HDR)-mediated gene editing methods to generate aglycosylated antibody-producing mice. Resulting aglycosylated antibodies showed required analytical performances without compromised protein stability. Thus, this aglycosylated monoclonal antibody-lectin coupled immunoassay for the quantification of tumour markers (ALIQUAT) method can provide a robust, versatile and accessible immunoassay platform to quantify specific glycoforms in precision cancer diagnostics. Moreover, the engineered mice can be used as a host to produce various aglycosylated antibodies in a convenient and robust fashion, thereby expanding in vitro diagnostic development opportunities that utilize glycoforms as a disease-specific biomarkers.
Dynamic networks observed in the nucleosome core particles couple the histone globular domains with DNA
Authors: Shi Xiangyan , Prasanna Chinmayi , Soman Aghil , Pervushin Konstantin , Nordenskiöld Lars 
Published: October 2020

AbstractThe dynamics of eukaryotic nucleosomes are essential in gene activity and well regulated by various factors. Here, we elucidated the internal dynamics at multiple timescales for the human histones hH3 and hH4 in the Widom 601 nucleosome core particles (NCP), suggesting that four dynamic networks are formed by the residues exhibiting larger-scale μs-ms motions that extend from the NCP core to the histone tails and DNA. Furthermore, despite possessing highly conserved structural features, histones in the telomeric NCP exhibit enhanced μs-ms dynamics in the globular sites residing at the identified dynamic networks and in a neighboring region. In addition, higher mobility was observed for the N-terminal tails of hH3 and hH4 in the telomeric NCP. The results demonstrate the existence of dynamic networks in nucleosomes, through which the center of the core regions could interactively communicate with histone tails and DNA to potentially propagate epigenetic changes.
Evaluating inositol phospholipid interactions with inward rectifier potassium channels and characterising their role in disease
Authors: Pipatpolkai Tanadet , Corey Robin A. , Proks Peter , Ashcroft Frances M. , Stansfeld Phillip J. 
Published: October 2020

AbstractMembrane proteins are frequently modulated by specific protein-lipid interactions. The activation of human inward rectifying potassium (hKir) channels by phosphoinositides (PI) has been well characterised. Here, we apply a coarse-grained molecular dynamics free-energy perturbation (CG-FEP) protocol to capture the energetics of binding of PI lipids to hKir channels. By using either a single- or multi-step approach, we establish a consistent value for the binding of PIP2 to hKir channels, relative to the binding of the bulk phosphatidylcholine phospholipid. Furthermore, by perturbing amino acid side chains on hKir6.2, we show that the neonatal diabetes mutation E179K increases PIP2 affinity, while the congenital hyperinsulinism mutation K67N results in a reduced affinity. We show good agreement with electrophysiological data where E179K exhibits a reduction in neomycin sensitivity, implying that PIP2 binds more tightly E179K channels. This illustrates the application of CG-FEP to compare affinities between lipid species, and for annotating amino acid residues.
Hydrogen release from a single water molecule on Vn+ (3 ≤ n ≤ 30)
Authors: Zhang Hanyu , Wu Haiming , Jia Yuhan , Yin Baoqi , Geng Lijun , Luo Zhixun , Hansen Klavs 
Published: October 2020

AbstractWater and its interactions with metals are closely bound up with human life, and the reactivity of metal clusters with water is of fundamental importance for the understanding of hydrogen generation. Here a prominent hydrogen evolution reaction (HER) of single water molecule on vanadium clusters Vn+ (3 ≤ n ≤ 30) is observed in the reaction of cationic vanadium clusters with water at room temperature. The combined experimental and theoretical studies reveal that the wagging vibrations of a V-OH group give rise to readily formed V-O-V intermediate states on Vn+ (n ≥ 3) clusters and allow the terminal hydrogen to interact with an adsorbed hydrogen atom, enabling hydrogen release. The presence of three metal atoms reduces the energy barrier of the rate-determining step, giving rise to an effective production of hydrogen from single water molecules. This mechanism differs from dissociative chemisorption of multiple water molecules on aluminium cluster anions, which usually proceeds by dissociative chemisorption of at least two water molecules at multiple surface sites followed by a recombination of the adsorbed hydrogen atoms.
Long-distance coupling and energy transfer between exciton states in magnetically controlled microcavities
Authors: Ściesiek Maciej , Sawicki Krzysztof , Pacuski Wojciech , Sobczak Kamil , Kazimierczuk Tomasz , Golnik Andrzej , Suffczyński Jan 
Published: October 2020

AbstractCoupling of quantum emitters in a semiconductor relies, generally, on short-range dipole-dipole or electronic exchange type interactions. Consistently, energy transfer between exciton states, that is, electron-hole pairs bound by Coulomb interaction, is limited to distances of the order of 10 nm. Here, we demonstrate polariton-mediated coupling and energy transfer between excitonic states over a distance exceeding 2 μm. We accomplish this by coupling quantum well-confined excitons through the delocalized mode of two coupled optical microcavities. Use of magnetically doped quantum wells enables us to tune the confined exciton energy by the magnetic field and in this way to control the spatial direction of the transfer. Such controlled, long-distance interaction between coherently coupled quantum emitters opens possibilities of a scalable implementation of quantum networks and quantum simulators based on solid-state, multi-cavity systems.
Funktionserholung nach Schlaganfall und die therapeutische Rolle der nicht-invasiven Hirnstimulation
Authors: Tscherpel Caroline , Grefkes Christian 
Published: October 2020

ZusammenfassungIm Bereich der non-invasiven Hirnstimulation stellen die transkranielle Magnetstimulation (engl. transcranial magnetic stimulation, TMS) sowie die transkranielle Gleichstromstimulation (engl. transcranial direct current stimulation, tDCS) bis heute die wichtigsten Techniken zur Modulation kortikaler Erregbarkeit dar. Beide Verfahren induzieren Nacheffekte, welche die Zeit der reinen Stimulation überdauern, und ebnen damit den Weg für ihren therapeutischen Einsatz beim Schlaganfall. In diesem Übersichtsartikel diskutieren wir die aktuelle Datenlage TMS- und tDCS-vermittelter Therapien für die häufigsten schlaganfallbedingten Defizite wie Hemiparese, Aphasie und Neglect. Darüber hinaus adressieren wir mögliche Einschränkungen der gegenwärtigen Ansätze und zeigen Ansatzpunkte auf, um Neuromodulation nach Schlaganfall effektiver zu gestalten und damit das Outcome der Patienten zu verbessern.
Simple Synthesis of Fused Thiazolo[4,5-b]pyridines through Successive SNAr Processes
Authors: Starosotnikov Alexey M. , Bastrakov Maxim A. , Kachala Vadim V. , Fedyanin Ivan V. , Klimova Tatyana A. , Ivanova Victoria V. , Dalinger Igor L. 
Published: October 2020

AbstractA convenient process is described for the synthesis of novel thiazolo[4,5-b]pyridines fused with triazole or pyrimidine rings. The base-promoted reactions of 2-chloro-3-nitropyridines with 1,3-(S,N)-binucleophiles (triazole-5-thiols, 4-oxopyrimidine-2-thiones) resulted in nucleophilic substitution of the chlorine atom and subsequent S–N-type Smiles rearrangement followed by nucleophilic substitution of the nitro group. Reactions with pyrimidine-2-thiones were carried out as one-pot processes while, in the case of triazole-5-thiols, isolation of intermediate substitution products was found to be preferable.
Avaliação funcional e de sintomatologia dolorosa do pé e tornozelo em indivíduos com obesidade grave – Estudo controlado transversal
Authors: Martins Glaucus Cajaty , Fraga Pedro Henrique Gomes , Teixeira Lucas Braga , Valle Bruno Rodrigues Guterres , Martins Filho Luiz Felippe , Gama Maurício de Pinho 
Published: October 2020

Resumo
Objetivo Avaliar a prevalência de queixas álgicas no pé e tornozelo, parâmetros radiográficos e o desempenho funcional de indivíduos com obesidade grave, Índice de Massa Corpórea (IMC) com valor > 40 e indicação de cirurgia bariátrica.
Métodos Foram avaliados 40 pacientes com obesidade grave acompanhados em ambulatório de cirurgia bariátrica. Este grupo de obesos graves (IMC > 40) foi subdividido em dois subgrupos: obesos com IMC < 50 (n = 24); e outro de obesos com IMC > 50 (n = 16). Foi realizada comparação com grupo controle de 42 indivíduo voluntários com IMC médio de 24. Foram avaliados a presença de dor no pé pela escala visual (EVA), o desempenho funcional pela escala da Associação Americana de Cirurgia do Pé e Tornozelo (AOFAS, na sigla em inglês) (domínios antepé, mediopé e retropé), idade, gênero, ângulo (âng) metatarso-falangeano do hálux, âng intermetatarsal do hálux , âng talocalcaneano, “pitch” calcaneano e âng de Meary.
Resultados Foi observada maior incidência de dor no pé no grupo de obesos graves em relação ao controle (p < 0,0001, razão de chances [odds ratio, OR]: 4,2). O desempenho funcional pela escala AOFAS foi inferior no grupo de obesos em relação ao controle (p < 0,0001, retropé com OR = 4,81; mediopé com OR = 3,33).
Conclusão Houve maior incidência de dor no pé no grupo de obesos graves em relação ao controle. Houve pior desempenho funcional pela escala AOFAS nas regiões do antepé, mediopé e retropé no grupo de obesos graves.
Relação entre sintomatologia no joelho e as características biológicas em corredores recreacionais
Authors: Ribeiro Paula Passuello Alves , Berni Kelly Cristina dos Santos 
Published: October 2020

Resumo
Objetivo O principal objetivo do presente estudo foi comparar a percepção subjetiva de dor e sintomas de dor anterior no joelho com as diferentes classificações de índice de massa corporal (IMC). O objetivo secundário foi verificar a associação entre as variáveis biológica e antropométrica com os resultados apresentados pelos sujeitos nos questionários subjetivos.
Métodos Foram recrutados 126 corredores recreacionais de ambos os gêneros, com idades entre 20 e 59 anos. Foram coletados dados referentes à variável biológica idade, e as variáveis antropométricas peso e altura, além da escala visual analógica (EVA) e os questionários Lysholm e Kujala. As informações foram obtidas por meio de plataforma digital, disponibilizado em um único link, para que fossem respondidos através de dispositivos eletrônicos pelos próprios voluntários. A normalidade foi verificada por meio do teste Shapiro-Wilk. Foi utilizado o teste-T e o teste de Wilcoxon para comparação das médias. A associação entre as variáveis foi determinada pela correlação linear de Pearson.
Resultados Houve diferença significativa entre a estatura do grupo sobrepeso e o grupo obesidade grau 1 (p = 0,029), e o peso do grupo peso normal para os grupos sobrepeso e obesidade grau 1 (p < 0,001), e entre as médias do IMC (p < 0,05). Foi observada correlação significativa não clara entre o IMC e os questionários específicos e a escala subjetiva (p < 0.05).
Conclusão Os corredores recreacionais que possuem IMC acima dos valores de normalidade estão mais predispostos a apresentar dor no joelho do que aqueles com IMC normal.