bluuebunny/crossref_metadata_2025_split huggingface dataset:
Stress response and detoxification mechanisms involved in non‐target‐site herbicide resistance in sunflower
Authors: Vega Tatiana , Gil Mercedes , Martin Gabriela , Moschen Sebastián , Picardi Liliana , Nestares Graciela 
Published: February 2020

AbstractThe nature of non‐target‐site herbicide resistance (NTSR) to imidazolinone (IMI) in HA425 sunflower (Helianthus annuus L.) has not yet been fully characterized but could be related to xenobiotic metabolism. The objective of this study was to evaluate the role of cytochrome P450 monooxygenases (P450s) and other detoxification‐related proteins in NTSR in sunflower. Two sunflower inbred lines were used: HA 425, which is IMI resistant (Imisun), and HA 89, which is IMI susceptible. The growth response to the IMI herbicide imazethapyr in combination with the P450 inhibitors 1‐aminobenzotriazole (ABT) or piperonyl butoxide (PBO) was evaluated in 15‐d‐old sunflower plantlets. Roots were collected, and label‐free quantitation (LFQ) proteomic analysis was carried out to characterize the NTSR mechanisms involved in the IMI resistance trait in sunflower. The increased phytotoxicity of imazethapyr observed in the resistant line when ABT or PBO were present agrees with the hypothesis that NTSR mechanisms may contribute to herbicide resistance in sunflower. The herbicide treatment also led to changes in the levels of biotic and abiotic stress‐related proteins, glutathione S‐transferases, and cytochrome P450s, among others. Plant growth and root protein expression response to IMI herbicides in sunflower would be a combination of stress‐related and detoxification mechanisms. Understanding the basis of NTSR becomes helpful to exploit this trait in sunflower crop and to develop xenobiotic‐resistant, soil‐remediating cultivars.
First EMC3‐EIRENE modelling of JT‐60SA edge plasmas with/without resonant magnetic perturbation field
Authors: Tanaka H. , Kawamura G. , Hoshino K. , Kobayashi M. , Matsunaga G. , Suzuki Y. , Lunt T. , Feng Y. , Ohno N. 
Published: February 2020

AbstractThe first EMC3‐EIRENE modelling for the JT‐60SA edge plasmas with/without the resonant magnetic perturbation field (RMP) was conducted. Both core degradation in the vacuum approximation and strike‐point splitting were observed due to the application of the RMP, as in previous studies in other devices. Toroidally averaged divertor heat fluxes with and without the RMP were fitted by using a one‐dimensional diffusive model function, showing similar profiles. To extract the modulation components of the divertor heat flux profile, the proper orthogonal decomposition (POD) method was applied. By using the POD outputs, a modulation ratio with respect to the toroidally averaged profile was quantitatively calculated.
Sacubitril/valsartan in patients listed for heart transplantation: effect on physical frailty
Authors: Cacciatore Francesco , Amarelli Cristiano , Maiello Ciro , Mattucci Irene , Salerno Gemma , Di Maio Marco , Palmieri Vittorio , Curcio Francesco , Pirozzi Flora , Mercurio Valentina , Benincasa Giuditta , Golino Paolo , Bonaduce Domenico , Napoli Claudio , Abete Pasquale 
Published: February 2020

AbstractAimsThe aim of this study was to investigate prospectively the effect of sacubitril/valsartan in advanced heart failure (HF) patients in waiting list for heart transplantation (HT) and the effect on physical frailty (PF).Methods and resultsWe treated 37 consecutive patients with advanced HF with sacubitril/valsartan. Patients were followed up until HT, device implant, or last follow‐up visit after 2 years of follow‐up. At baseline, mean New York Heart Association (NYHA) class was 3.1 ± 0.4, with 64.9% in NYHA III and 35.1% NYHA IIIB. Left ventricular ejection fraction was 23.5 ± 5.8%, VO2 max was 10.3 ± 2.3 mL/kg/min, cardiac index was 2.3 ± 0.5 L/min/m2, and N‐terminal pro‐brain natriuretic peptide (NT‐pro‐BNP) was 4943.0 ± 5326.8 pg/mL. After a mean follow‐up of 17.1 ± 4.4 months, no deaths were observed, but NYHA class improved significantly with 56.8% in NYHA II, 40.5% in NYHA III, and 2.7% in NYHA IIIB (P < 0.001). VO2 max and 6 min walk test (6MWT) increased, whereas pulmonary systolic blood pressure, E/E′, VE/VCO2 slope, and NT‐pro‐BNP decreased. At right heart catheterization performed after 1 year of follow‐up, cardiac index and pulmonary vascular resistance remained stable, while a decrease in systolic pulmonary artery pressure and pulmonary capillary wedge pressure is observed. Furosemide dosage decrease from 102.7 ± 69.4 to 78.7 ± 66.3 mg (P = 0.040). PF decreased from 3.35 ± 1.0 at baseline to 1.57 ± 1.3 at the end of follow‐up (P < 0.001), with a reduction in all PF domains.ConclusionsOur study showed a rapid improvement in PF in HT waiting list patients treated with sacubitril/valsartan. The improvement in all PF domains was paralleled by VO2 and 6MWT increase and together with an NT‐pro‐BNP reduction constant over the follow‐up.
Antimony Doped Tin Oxide Nanoparticles Deposited onto Nb−TiO2 Nanotubes for Electrochemical Degradation of Bio‐refractory Pollutions
Authors: Xu Li , Yi Yan , Liang Gaorui , Zhang Wen 
Published: February 2020

AbstractTo improve the service life of SnO2−Sb electrodes in degradation of refractory wastewater, we report anodic information of tin oxide antimony on top of Nb−TiO2 nanotubes (Nb−Ti/Nb−TiO2−NTs/ATONPs) prepared through screen‐printing. It was found that the Nb−Ti/Nb−TiO2−NTs/ATONPs anodes presented a significantly enhanced in electro‐catalytic oxidation performance (in Acid Red 73) compared to titanium‐based tin antimony electrodes (Ti/ATONPs). Additionally, the electrochemical properties and the stability were further studied by the electrochemical impedance spectroscopy (EIS), linear sweep voltammetry (LSV), cyclic voltammetry (CV), chronoamperometry (CA) measurements and accelerated life test, respectively. These results indicated that Nb−TiO2−NTs/ATONPs anode possessed Nb−TiO2 nanotubes which exhibited a higher oxygen evolution potential (2.24 V vs. Ag/AgCl), as well as a better wettability, a larger current at constant potential and 2.1 times longer lifetime than the conventional Ti/ATONPs anode.
Interspecific Variation in Nematode Responses to Metals
Authors: Heaton Andrew , Faulconer Elizabeth , Milligan Emma , Kroetz Mary B. , Weir Scott M. , Glaberman Scott 
Published: February 2020

Abstract
Performing toxicity testing on multiple species with differing degrees of evolutionary relatedness can provide important information on how chemical sensitivity varies among species and can help pinpoint the biological drivers of species sensitivity. Such knowledge could ultimately be used to design better multispecies predictive ecological risk assessment models and identify particularly sensitive species. However, laboratory toxicity tests involving multiple species can also be resource intensive, especially when each species has unique husbandry conditions. We performed lethality tests with 2 metals, copper chloride and zinc chloride, on 5 different nematode species, which are nested in their degree of evolutionary relatedness: Caenorhabditis briggsae, Caenorhabditis elegans, Oscheius myriophila, Oscheius tipulae, and Pristionchus pacificus. All species were successfully cultured and tested concurrently with limited resources, demonstrating that inexpensive, multispecies nematode toxicity testing systems are achievable. The results indicate that P. pacificus is the most sensitive to both metals. Conversely, C. elegans is the least sensitive species to copper, but the second most sensitive to zinc, indicating that species relationships do not necessarily predict species sensitivity. Toxicity testing with additional nematode species and types of chemicals is feasible and will help form more generalizable conclusions about relative species sensitivity. Environ Toxicol Chem 2020;39:1006–1016. © 2020 SETAC
A Comparative Study of Wavelet‐based Descriptors for Fault Diagnosis of Self‐Humidified Proton Exchange Membrane Fuel Cells
Authors: Sethi A. , Verstraete D. 
Published: February 2020

AbstractFault diagnosis can help extend the lifetime of fuel cells and has been widely explored for externally humidified fuel cells. However, fault detection in self‐humidified fuel cells is scarce, despite its importance. This paper explores various wavelet‐based descriptors for identifying hydration levels in self‐humidified stacks. Wavelet‐based techniques are non‐intrusive and provide concurrent examinations in time and frequency. Thus, they encapsulate health related data that can be used as health monitoring features. The specific wavelet‐based techniques used here are (i) impedance obtained through the wavelet‐based fast electrochemical impedance spectroscopy (EIS) and (ii) changes in wavelet energy through wavelet and wavelet packet decomposition. Results are compared based on their trends under dehydration conditions and the robustness of those trends across different currents and sampling rates. They are then assessed based on their predictive ability using RReliefF (Regression‐ReliefF)‐ a regression based feature ranking tool. Based on the qualitative and quantitative analysis, descriptors are assessed in the context of creating a short‐term health monitoring system for self‐humidified fuel cells. While a modified version of the wavelet energies provides the best results for differentiating faults, fast EIS opens the possibility for analysis of more complex fault modes and is shown to be more robust.
Genetics and epigenetics of leukemia and lymphoma: from knowledge to applications, meeting report of the Josep Carreras Leukaemia Research Institute
Authors: Parra Maribel , Baptista Maria Joao , Genescà Eulàlia , Llinàs‐Arias Pere , Esteller Manel 
Published: February 2020

AbstractThe meeting, which brought together leading scientists and clinicians in the field of leukemia and lymphoma, was held at the new headquarters of the Josep Carreras Leukaemia Research Institute (IJC) in Badalona, Catalonia, Spain, September 19‐20, 2019. Its purpose was to highlight the latest advances in our understanding of the molecular mechanisms driving blood cancers, and to discuss how this knowledge can be translated into an improved management of the disease. Special emphasis was placed on the role of genetic and epigenetic heterogeneity, and the exploitation of epigenetic regulation for developing biomarkers and novel treatment approaches.
The role of M2‐2 PDZ‐binding motifs in pulmonary innate immune responses to human metapneumovirus
Authors: Choi Eun‐Jin , Wu Wenzhe , Chen Yu , Yan Weiyu , Li Liqing , Choudhury Atanu , Bao Xiaoyong 
Published: February 2020

AbstractHuman metapneumovirus (HMPV) is a leading cause of lower respiratory tract infection (LRTI) in pediatric and geriatric populations. We recently found that two PDZ‐binding motifs of the M2‐2 protein, 29‐DEMI‐32 and 39‐KEALSDGI‐46, play a significant role in mediating HMPV immune evasion in airway epithelial cells (AECs). However, their role in the overall pulmonary responses to HMPV infection has not been investigated. In this study, we found that two recombinant HMPVs (rHMPV) lacking the individual M2‐2 PDZ‐binding motif are attenuated in mouse lungs. Mice infected with mutants produce more cytokines/chemokines in bronchoalveolar lavage (BAL) fluid compared to mice infected with wild‐type rHMPV. In addition, both mutants are able to enhance the pulmonary recruitment of dendritic cells (DCs) and T cells and induce effective protections against the HMPV challenge. The DC maturation is also significantly improved by the motif mutation. Taken together, our data provide proof‐of‐principle for two live‐attenuated M2‐2 mutants to be promising HMPV vaccine candidates that are effective in inducing higher pulmonary innate immunity and generating protection against HMPV infection.
Antibodies to coronaviruses are higher in older compared with younger adults and binding antibodies are more sensitive than neutralizing antibodies in identifying coronavirus‐associated illnesses
Authors: Gorse Geoffrey J. , Donovan Mary M. , Patel Gira B. 
Published: February 2020

AbstractHuman coronaviruses (HCoV) are common causes of respiratory illnesses (RI) despite preexisting humoral immunity. Sera were obtained near the onset of RI and 3 to 4 weeks later as part of a prospective study of 200 subjects evaluated for RI from 2009 to 2013. Antibodies against common HCoV strains were measured by enzyme‐linked immunosorbent assay and neutralization assay comparing older adults with cardiopulmonary diseases (99 subjects) to younger, healthy adults (101 subjects). Virus shedding was detected in respiratory secretions by polymerase chain reaction. Of 43 HCoV‐associated illnesses, 15 (35%) occurred in 14 older adults (aged ≥60 years) and 28 (65%) in 28 younger adults (aged 21‐40 years). Binding and neutralizing antibodies were higher in older adults. Only 16 (35.7%) of RI with increases in binding antibodies also had increases in neutralizing antibodies to HCoV. Increases in binding antibodies with RI were more frequent than increased neutralizing antibodies and virus shedding, and more frequent in younger compared to older adults. Functional neutralizing antibodies were not stimulated as often as binding antibodies, explaining in part a susceptibility to reinfection with HCoV. Monitoring binding antibodies may be more sensitive for the serologic detection of HCoV infections.
Exploiting the versatility of vacuum‐assisted headspace solid‐phase microextraction in combination with the selectivity of ionic liquid‐based GC stationary phases to discriminate Boswellia spp. resins through their volatile and semivolatile fractions
Authors: Capetti Francesca , Rubiolo Patrizia , Bicchi Carlo , Marengo Arianna , Sgorbini Barbara , Cagliero Cecilia 
Published: February 2020

AbstractThe frankincense resins, secreted from Boswellia species, are an uncommon example of a natural raw material where every class of terpenoids is present in similar proportions. Diterpenoids (serratol, incensole, and incensole acetate) are used to discriminate samples from different species and origins. Headspace solid‐phase microextraction has been used for frankincense analysis, although it requires long sampling time for medium‐ to low‐volatility markers; headspace solid‐phase microextraction under vacuum can overcome this limit. Gas chromatography is used for analysis but the separation of incensole and serratol needs polar stationary phases. In this study, we develop a method to discriminate frankincenses based on vacuum‐assisted headspace solid‐phase microextraction combined with fast gas chromatography‐mass spectrometry with ionic liquid–based stationary phases. The optimized conditions for solid samples were: air evacuation below 0°C, 15 min of incubation time, and 15 min of extraction time. Losses of volatiles due to vial air‐evacuation in the presence of the sample were minimized by sample amount above 100 mg and low sample temperature. Fast gas chromatography provides the baseline separation of all markers in 20 min. By applying vacuum sampling and fast gas chromatography, the total analysis was reduced to 50 min compared to 120 min (60 min sampling plus 60 min analysis) as previously reported. The method was successfully applied to commercial frankincense samples.
Respiratory Laryngeal Dystonia: Characterization and Diagnosis of a Rare Neurogenic Disorder
Authors: Tierney William S. , Bryson Paul C. , Nelson Rebecca , Kaplan Seth E. , Benninger Michael S. , Milstein Claudio F. 
Published: February 2020

Objectives/HypothesisRespiratory laryngeal dystonia (RLD) is poorly understood and rarely reported in the literature. Patients have atypical laryngeal movement resulting in airway obstruction. This motion is neurogenic in nature, is constant while awake, nonepisodic, and non–trigger dependent. Given its rarity, it is often misdiagnosed for inducible laryngeal obstruction; however, it is refractory to medical and behavioral management. Although this condition has been addressed in the literature, this report is the largest case series characterizing presenting symptomology, multimodal treatment outcomes, and longitudinal course of these patients, and proposes a set of diagnostic criteria to aid in clinical identification of RLD patients. Our objectives were to characterize RLD clinically and offer diagnostic guidelines to clinicians.Study DesignA prospective case series with a retrospective analysis at a tertiary referral center.MethodsA review of clinical records and videostroboscopic analysis of 16 patients treated for respiratory laryngeal dystonia from October 2005 to October 2018 was performed.ResultsSixteen patients with respiratory laryngeal dystonia were included. The common features of this group were persistent, nonepisodic dyspnea and stridor with laryngoscopic evidence of paradoxical vocal fold motion. Our patients had no structural neurologic abnormalities. These patients typically failed respiratory retraining therapy and medical management of laryngeal irritants. In our series, 100% of patients underwent respiratory retraining therapy, 68.8% received laryngeal botulinum toxin injection, and 31.3% required tracheostomy.ConclusionsRLD is a rare and challenging condition. The disorder can be severely disabling, and treatment options appear limited. A multidisciplinary approach may be helpful. Some patients responded to laryngeal botulinum injection and medical management, whereas others required tracheostomy for symptom control. Laryngoscope, 2020
Fatty Acyl Synthetases and Thioesterases in Plant Lipid Metabolism: Diverse Functions and Biotechnological Applications
Authors: Kalinger Rebecca S. , Pulsifer Ian P. , Hepworth Shelley R. , Rowland Owen 
Published: February 2020

AbstractPlants use fatty acids to synthesize acyl lipids for many different cellular, physiological, and defensive roles. These roles include the synthesis of essential membrane, storage, or surface lipids, as well as the production of various fatty acid‐derived metabolites used for signaling or defense. Fatty acids are activated for metabolic processing via a thioester linkage to either coenzyme A or acyl carrier protein. Acyl synthetases metabolically activate fatty acids to their thioester forms, and acyl thioesterases deactivate fatty acyl thioesters to free fatty acids by hydrolysis. These two enzyme classes therefore play critical roles in lipid metabolism. This review highlights the surprisingly complex and varying roles of fatty acyl synthetases in plant lipid metabolism, including roles in the intracellular trafficking of fatty acids. This review also surveys the many specialized fatty acyl thioesterases characterized to date in plants, which produce a great diversity of fatty acid products in a tissue‐specific manner. While some acyl thioesterases produce fatty acids that clearly play roles in plant‐insect or plant‐microbial interactions, most plant acyl thioesterases have yet to be fully characterized both in terms of their substrate specificities and their functions. The biotechnological applications of plant acyl thioesterases and synthetases are also discussed, as there is significant interest in these enzymes as catalysts for the sustainable production of fatty acids and their derivatives for industrial uses.
Take me on a ride: The role of environmentalist identity for carpooling
Authors: Hartl Barbara , Kamleitner Bernadette , Holub Sandra 
Published: February 2020

AbstractSharing does not need to involve corporate providers but can also happen on a peer‐to‐peer (P2P) basis. P2P sharing platforms who match private providers and users are thus dealing with two different customer segments. An example of this is carpooling, the sharing of a car journey. Recent years have seen considerable research on why people use sharing services. In contrast, there is little knowledge of why people may offer a good for sharing purposes. Drawing on identity theory, this paper suggests that users and providers of carpooling need to be addressed differently. A pilot study and two studies, including both actual car owners and nonowners confirm that the extent to which one identifies as an environmentalist predicts car owners' willingness to offer carpooling, but does not affect nonowners' willingness to use carpooling services. These findings remain robust when controlling for various potential confounds. Furthermore, Study 2 suggests that an environmentalist identity plays an important role for car owners' actual decision to offer a ride via an online platform. These results suggest that marketers of P2P platforms need to pursue different strategies when addressing potential users and providers on the same platform.
Laser cladding nickel‐titanium carbide composite coating on a 45 carbon steel: Preparation, microstructure and wear behavior
Authors: Zhu C. , Zhang B. , Zheng S. 
Published: February 2020

AbstractAlmost fully dense nickel‐titanium carbide composite coatings with varied titanium carbide content were deposited on 45 carbon steel by laser cladding. High content of titanium carbide particles up to 50 wt.% with bimodal microstructure could be homogeneously distributed in the nickel based matrix. Due to the presence of the harder nickel‐titanium carbide composite coating on the 45 carbon steel, the surface hardness and wear properties were significantly improved. The Vickers hardness (HV 3) increased from about 260 HV 3 for the 45 carbon steel to 300 HV 3 – 360 HV 3 for nickel based composite coating containing 30 wt.% titanium carbide and 550 HV 3 – 680 HV 3 for nickel based composite coating containing 50 wt.% titanium carbide composite coating, respectively. The coefficient of friction and volume wear rate was reduced down to 0.41×10−6 mm3 N−1 m−1 and 9.3×10−6 mm3 N−1 ⋅ m−1 when a nickel based composite coating containing 50 wt.% titanium carbide was coated on the 45 carbon steel, respectively. The enhanced wear performance of the composite coating was due to presence of harder nickel‐titanium carbide composite coating and formation of varied soft and lubricant metal oxides consisting of mainly titanium oxides and minor iron and nickel oxides.
Desire to lose weight and need of weight loss support in the adult population—Results from a cross‐sectional study in Sweden
Authors: Molarius Anu , Lindén‐Boström Margareta , Karlsson Jan 
Published: February 2020

SummaryObjectiveEvidence‐based methods to lose weight are important in tackling increasing obesity trends in adult populations. More knowledge about persons who want to lose weight and do not/do need weight loss support is necessary to design effective preventive practices. Thus, the aim of the study was to investigate the prevalence of desire to lose weight in the general population and the prevalence of health problems and health‐related factors in persons with overweight or obesity who want to lose weight and believe that they do not/do need weight loss support.MethodsThe study included 14 126 persons aged 30 to 69 years who responded to a questionnaire sent to a random sample. Persons with overweight or obesity (BMI ≥ 25 kg/m2) were divided into three groups: those who do not want to lose weight (n = 1236), those who want to lose weight but do not believe they need support (n = 5484), and those who want to lose weight and believe they need weight loss support (n = 1462).ResultsIn total, 69% of the women and 59% of the men reported that they wanted to lose weight. The prevalence of hypertension, musculoskeletal pain, poor self‐rated health, anxiety/worry, and depression was highest among persons with overweight or obesity who wanted to lose weight and believed they need weight loss support. They were also more physically inactive and reported less social support.ConclusionsTo want to lose weight is very common among adults. People with overweight or obesity who want to lose weight and believe they need weight loss support have higher frequency of various health problems, including mental health problems, and less social support.