bluuebunny/crossref_metadata_2025_split huggingface dataset:
A novel monogenic preimplantation genetic testing strategy for sporadic polycystic kidney caused by de novoPKD1 mutation
Authors: Shi Hao , Niu Wenbin , Liu Yidong , Jin Haixia , Song Wenyan , Shi Senlin , Yao Guidong , Xu Jiawei , Sun Yingpu 
Published: October 2020

AbstractAutosomal dominant hereditary polycystic kidney disease (ADPKD) is the most common inherited kidney disease that causes end‐stage renal disease and kidney failure. Preimplantation genetic testing for monogenic (PGT‐M) can effectively prevent the transmission of genetic diseases from parents to the offspring before pregnancy. However, PGT‐M currently adopts the single nucleotide polymorphism (SNP) linkage analysis for embryo's pathogenic gene carrying status and linkage analysis requires proband of the family. Here we report a new PGT‐M strategy using single sperm SNP linkage analysis for male patient with sporadic ADPKD caused by de novo PKD1 mutation. We recruited five couples with male patient with ADPKD caused by de novo PKD1 mutation, and 39 embryos from six PGT‐M cycles were detected. The five couples had at least one embryo that does not carry the PKD1 mutation. Within these five couples, the accuracy of carrier status of embryos was confirmed by amniotic fluid gene detection of two couples and two couples successfully delivered healthy fetuses. Therefore, the new PGT‐M strategy of using single sperm SNP linkage analysis was proved to be feasible and effective for male patient with ADPKD caused by de novo PKD1 mutation.
Metaplastic carcinomas of the breast without evidence of epithelial differentiation: a diagnostic approach for management
Authors: Rakha Emad A , Quinn Cecily M , Foschini Maria Pia , Muñoz Martin Monica , Dabbs David J , Lakhani Sunil , Varga Zsuzsanna , Pinder Sarah E , Schmitt Fernando C , Reis‐Filho Jorge S , Fox Stephen B , Ellis Ian O , Tan Puay‐Hoon , Mihai Raluca 
Published: October 2020

AimsAlthough rare, malignant sarcomatoid breast tumours without evidence of epithelial differentiation comprise a diagnostic challenge with management implications. Earlier studies have generally considered these to be primary breast sarcomas; however, supporting evidence is lacking and management remains variable. This study aimed to provide an evidence‐based approach to improve the consistency of diagnosis and management for such cases.Methods and resultsA large series (n = 140) of metaplastic breast carcinoma (MBC) diagnosed in Nottingham over 18 years was analysed. Only cases with available data on immunohistochemical expression of cytokeratins (CKs) were included. The prevalence and pattern of expression for various CKs were assessed and details of tumours negative for CKs were collected. A diagnostic approach based on our experience is provided. Forty‐seven cases (34%) showed foci of conventional type invasive breast carcinoma or ductal carcinoma in situ (DCIS), while 93 cases (66%) were diagnosed as MBC based on morphology and/or CK expression. Ninety‐seven cases (69%) were negative for one or more CKs, with 18 cases (13%) negative for five or more CKs. Eight cases (6%) lacked expression of all CKs tested. Further examination showed evidence of carcinomatous nature in five cases, and three were diagnosed as MBC following extensive diagnostic work‐up and based on our experience.ConclusionThis study suggests that MBC represents a spectrum of neoplasms, with some lacking CK expression. Sarcomatoid neoplasms of the breast lacking evidence of carcinomatous morphology and CK expression may represent an extreme end of differentiation that can be considered as carcinomas rather than sarcomas for management purposes (following extensive work‐up).
Genetic parameters and direct and maternal effects for important traits in late‐feathering Qingyuan partridge hens in China
Authors: Kang Huimin , Li Shanshan , Li Hua , Gan Jiankang , Luo Jieling , Zhang Zhengfen , Deng Xuqing , Wei Jindui , Li Gen 
Published: October 2020

AbstractQingyuan partridge chicken is an important indigenous chicken in China. In its breeding schemes, chickens are usually selected at the age of 105‐day‐old for five traits, including body weight (BW), shank length (SL), shank girth (SG), comb height (CH) and feather maturity (FM). At present, genetic parameters of the aforementioned traits have still not been studied in Qingyuan partridge chickens. The objectives of this study were the following: (1) to investigate whether the optimal statistical models of these traits need to consider maternal genetic and permanent environmental effects in late‐feathering Qingyuan partridge hens, and (2) to estimate genetic parameters for these traits based on the optimal models. The numbers of records for BW, SL, SG, CH and FM were 13,721, 13,671, 13,670, 13,669 and 13,672, respectively. Variance components were estimated using average information‐restricted maximum likelihood method, and the optimal model was determined based on Bayesian information criterion. More specifically, the optimal model for BW considered maternal genetic and permanent environmental effects in addition to direct additive genetic effect; SL, SG and FM considered direct and maternal genetic effects; and CH considered direct and maternal genetic effects, and the covariance between them. The direct heritabilities of these traits estimated using the optimal models were 0.21 ± 0.04, 0.30 ± 0.05, 0.40 ± 0.05, 0.59 ± 0.09 and 0.09 ± 0.04, respectively; the maternal heritabilities were 0.01 ± 0.04, 0.05 ± 0.05, 0.04 ± 0.05, 0.09 ± 0.09 and 0.03 ± 0.04, respectively. Maternal genetic effect evidently played an important part in FM and maternal heritability accounted for 30 per cent of total heritability. Furthermore, the direct and maternal genetic effects for CH were estimated to be negatively and moderately correlated (−0.51 ± 0.11). For all traits, neglecting existent maternal effects biased the estimation of direct heritability. Therefore, to implement optimum breeding strategies for improvement of these traits in Qingyuan partridge hens, maternal effects should be taken into consideration.
U.S. health insurance marketplace taxonomy and the influence of labeling on consumer perception of plan suitability
Authors: Hickey Christopher , Tharp Derek T. 
Published: October 2020

AbstractThis study investigates whether metal categories used in the health insurance marketplace established by the Affordable Care Act influence consumer choice in plan selection. Participants (N = 598) were randomly assigned to a marketplace environment containing either neutral terminology, metal categories, or descriptive labels. Respondents were asked to choose the most appropriate plan for low‐, average‐, and high‐use cases of health care consumption. Despite the bronze plan possessing the lowest estimated cost across each use case, the use of precious metal categories (compared to a neutral control) resulted in lower frequency selection of the gold plan in a low‐use case, higher frequency selection of the silver plan in an average‐use case, and higher frequency selection of the gold plan in a high‐use case. Furthermore, this study finds that beliefs equating more insurance with better health care and normative beliefs regarding the relationship between income and insurance are important predictors of plan selection.
Acquisition mode and credit card overspending behavior: An empirical analysis of the credit card industry
Authors: Ming Yaxin , Li Chenxi , Chen Jing (Elaine) 
Published: October 2020

AbstractAlthough credit card overspending behavior has become a critical societal concern with severe negative impacts on consumer welfare and economic stability, research on credit card overspending behavior remains fragmented and understudied. This study investigates new types of antecedents of credit card overspending behavior that are overlooked in the prior literature—the acquisition mode of credit card companies. Based on data set from a large commercial bank in China, this study measures credit card overspending behavior using consumption amount, cash withdrawal amount and overdueness, and suggests that gift acquisition has a positive effect on cash withdrawal amount and overdue probability as well as a negative effect on consumption amount. Furthermore, we find that this relationship could be weakened for female customers and for customers with higher education levels. This study provides theoretical implications for both the credit card overspending literature and customer acquisition literature. It also has important implications for consumer welfare and public policymaking.
The work environment of haemodialysis nurses and its mediating role in burnout
Authors: Moisoglou Ioannis , Yfantis Aris , Tsiouma Erasmia , Galanis Petros 
Published: October 2020

AbstractBackgroundThe work environment can contribute to the occurrence of nurses' burnout.ObjectivesThis study assessed the work environment of haemodialysis nurses and the extent of burnout they have suffered, and it explored the connection between work environment and burnout.DesignA multicentre cross‐sectional study was conducted in 53 public, hospital‐based haemodialysis units in Greece.ParticipantsA total of 537 haemodialysis nurses participated. The majority consisted of females (90.8%) who possessed a university degree or a degree from a technological institute (72.6%). They had an average of 15 years of experience in dialysis.MeasurementsThe Practice Environment Scale of the Nursing Work Index and the Copenhagen Burnout Inventory (CBI) were used. The demographic characteristics of the participants were also collected.ResultsAlthough the nurses rated their work environment as healthy, they were experiencing a moderate degree of burnout. The multivariate linear regression analysis with the CBI scales as dependent variables was applied. The increased nurses' participation in hospital affairs was associated with decreased personal (coefficient β = −9.4, 95% confidence interval [CI] = −17.4 to −1.3, p = 0.023), work‐related (coefficient β = −12.9, 95% CI = −22.5 to −3.5, p = 0.008) and patient‐related burnout (coefficient β = −15.9, 95% CI = −24.5 to −7.4, p = < 0.001). The better collegial nurse–physician relationships were associated with decreased personal (coefficient β = −10.7, 95% CI = −18.3 to −3.0, p = .007) and work‐related burnout (coefficient β = −15.1, 95% CI = −23.3 to −7.0, p =  < 0.001).ConclusionsThe work environment of haemodialysis nurses was associated with burnout. Its improvement is an important tool in administrations' effort to ensure the nursing staff's well‐being.
Endozoochory of the same community of plants lacking fleshy fruits by storks and gulls
Authors: Martín‐Vélez Víctor , Lovas‐Kiss Ádám , Sánchez Marta I , Green Andy J 
Published: October 2020

AbstractAimsResearch into the dispersal of plants lacking a fleshy fruit by avian endozoochory remains limited, particularly regarding the different roles of specific vectors in the same habitat.MethodsWe compared plants dispersed by endozoochory between two migratory waterbirds differing in body size: the lesser black‐backed gull Larus fuscus, and the white stork Ciconia ciconia. We collected faeces and pellets from roosting flocks on dykes in rice fields in Doñana, SW Spain, and extracted intact seeds.ResultsWe recovered 424 intact seeds from excreta, representing 21 plant taxa, 11 of which germinated under laboratory conditions. Eight plant species are considered weeds, four of them as alien species, and only two have a fleshy fruit. Seed abundance and species richness per sample did not differ between storks and gulls. Toadrush (Juncus bufonius) was the dominant species, accounting for 49% of seeds recovered. PERMANOVA and mvabund analyses revealed no differences in the proportions of each plant species dispersed by the two vectors, and seasonal variation in abundance was absent. Overall, germinability was 19%, and declined with increasing delay between sample collection and processing. Transects along dykes identified 52 plant taxa, only 18 of which were recorded in excreta.ConclusionsOverlap in the communities of non‐fleshy‐fruited plants dispersed by two unrelated birds of different size suggests that waterbird plant dispersal networks are different from frugivore networks. Unlike for frugivores, decoupling between seed production and ingestion reduces seasonal variation in endozoochory rates. For Juncus bufonius and other plants, these avian vectors provide maximum dispersal distances several orders of magnitude greater than predicted from their dispersal syndromes. Endozoochory by migratory waterbirds has major implications for plant distributions in a rapidly changing world, and more research is required before we can predict which plants disperse regularly via this mechanism.
Zooming on dynamics of marine microbial communities in the phycosphere of Akashiwo sanguinea (Dinophyta) blooms
Authors: Kang Junsu , Park Joon Sang , Jung Seung Won , Kim Hyun‐Jung , Joo Hyoung Min , Kang Donhyug , Seo Hyojeong , Kim Sunju , Jang Min‐Chul , Lee Kyun‐Woo , Jin Oh Seok , Lee Sukchan , Lee Taek‐Kyun 
Published: October 2020

AbstractCharacterizing ecological relationships between viruses, bacteria and phytoplankton in the ocean is critical to understanding the ecosystem; however, these relationships are infrequently investigated together. To understand the dynamics of microbial communities and environmental factors in harmful algal blooms (HABs), we examined the environmental factors and microbial communities during Akashiwo sanguinea HABs in the Jangmok coastal waters of South Korea by metagenomics. Specific bacterial species showed complex synergistic and antagonistic relationships with the A. sanguinea bloom. The endoparasitic dinoflagellate Amoebophrya sp. 1 controlled the bloom dynamics and correlated with HAB decline. Among nucleocytoplasmic large DNA viruses (NCLDVs), two Pandoraviruses and six Phycodnaviruses were strongly and positively correlated with the HABs. Operational taxonomic units of microbial communities and environmental factors associated with A. sanguinea were visualized by network analysis: A. sanguinea–Amoebophrya sp. 1 (r = .59, time lag: 2 days) and A. sanguinea–Ectocarpus siliculosus virus 1 in Phycodnaviridae (0.50, 4 days) relationships showed close associations. The relationship between A. sanguinea and dissolved inorganic phosphorus relationship also showed a very close correlation (0.74, 0 day). Microbial communities and the environment changed dynamically during the A. sanguinea bloom, and the rapid turnover of microorganisms responded to ecological interactions. A. sanguinea bloom dramatically changes the environments by exuding dissolved carbohydrates via autotrophic processes, followed by changes in microbial communities involving host‐specific viruses, bacteria and parasitoids. Thus, the microbial communities in HAB are composed of various organisms that interact in a complex manner.
Plastidial transporters KEA1 and KEA2 at the inner envelope membrane adjust stromal pH in the dark
Authors: Aranda Sicilia María Nieves , Sánchez Romero María Elena , Rodríguez Rosales María Pilar , Venema Kees 
Published: October 2020

Summary

Photosynthesis and carbon fixation depend critically on the regulation of pH in chloroplast compartments in the daylight and at night. While it is established that an alkaline stroma is required for carbon fixation, it is not known how alkaline stromal pH is formed, maintained or regulated.

We tested whether two envelope transporters, AtKEA1 and AtKEA2, directly affected stromal pH in isolated Arabidopsis chloroplasts using the fluorescent probe 2ʹ,7ʹ‐bis‐(2‐carboxyethyl)‐5‐(and‐6)‐carboxyfluorescein (BCECF).

External K+‐induced alkalinization of the stroma was observed in chloroplasts from wild‐type (WT) plants but not from kea1kea2 mutants, suggesting that KEA1 and KEA2 mediate K+ uptake/H+ loss to modulate stromal pH. While light‐stimulated alkalinization of the stroma was independent of KEA1 and KEA2, the rate of decay to neutral pH in the dark is delayed in kea1kea2 mutants. However, the dark‐induced loss of a pH gradient across the thylakoid membrane was similar in WT and mutant chloroplasts.

This indicates that proton influx from the cytosol mediated by envelope K+/H+ antiporters contributes to adjustment of stromal pH upon light to dark transitions.
Assessing person‐centred care: An item response theory approach
Authors: Kazemi Ali , Kajonius Petri 
Published: October 2020

AbstractBackground and ObjectivesGiven recent advances in psychometric assessment, there is a need for assessment studies using modern test theory in the field of person‐centred care, mainly due to the dominant use of analytical strategies based on classical test theory. The main objective of the present study was thus to examine whether selected items from commonly used instruments of person‐centred care were able to differentiate between respondents with a reasonably even level of measurement precision across different regions of the construct range using item response theory (IRT).Research Design and MethodsA Swedish sample of care staff in elderly care (N = 1342) completed a survey including a selection of items from three previously validated measures of person‐centred care.ResultsAll questionnaire items were submitted to IRT analyses to examine the extent to which the items produced information on the underlying construct. The items exhibited different levels of information. However, in general, for those items exhibiting some information, the pattern of information across the trait range was similar for most of them, that is, the items discriminated better in the lower levels of person‐centredness.Discussion and ImplicationsItem response theory analyses are instrumental in creating shorter measurement instruments that may perform nearly as well as the original longer instruments. Given time and other resource constraints in questionnaire administration, there is a gain in only including the most informative items which efficiently and evenly tap the underlying construct along its entire range and in the context of person‐centred care assessment this study was an initial step towards this goal. Thus, a set of ten items with satisfactory levels of psychometric quality, that is relatively high information levels across a relatively broad range of the underlying construct, is proposed.
Modulation of physiological performance by temperature and salinity in the sugar kelp Saccharina latissima
Authors: Monteiro Cátia , Li Huiru , Diehl Nora , Collén Jonas , Heinrich Sandra , Bischof Kai , Bartsch Inka 
Published: October 2020

SUMMARYThe sugar kelp Saccharina latissima experiences a wide range of environmental conditions along its geographical and vertical distribution range. Temperature and salinity are two critical drivers influencing growth, photosynthesis and biochemical composition. Moreover, interactive effects might modify the results described for single effects. In shallow water coastal systems, exposure to rising temperatures and low salinity are expected as consequence of global warming, increased precipitation and coastal run‐off. To understand the acclimation mechanisms of S. latissima to changes in temperature and salinity and their interactions, we performed a mechanistic laboratory experiment in which juvenile sporophytes from Brittany, France were exposed to a combination of three temperatures (0, 8 and 15°C) and two salinity levels (20 and 30 psu (practical salinity units)). After a temperature acclimation of 7 days, sporophytes were exposed to low salinity (20 psu) for a period of 11 days. Growth, and maximal quantum yield of photosystem II (Fv/Fm), pigments, mannitol content and C:N ratio were measured over time. We report for the first time in S. latissima a fivefold increase in the osmolyte mannitol in response to low temperature (0°C) compared to 8 and 15°C that may have ecological and economic implications. Low temperatures significantly affected all parameters, mostly in a negative way. Chlorophyll a, the accessory pigment pool, growth and Fv/Fm were significantly lower at 0°C, while the de‐epoxidation state of the xanthophyll cycle was increased at both 0 and 8°C compared to 15°C. Mannitol content and growth decreased with decreased salinity; in contrast, pigment content and Fv/Fm were to a large extent irresponsive to salinity. In comparison to S. latissima originating from an Arctic population, despite some reported differences, this study reveals a remarkably similar impact of temperature and salinity variation, reflecting the large degree of adaptability in this species.
Agricultural land partitioning model based on irrigation efficiency using a multi‐objective artificial bee colony algorithm
Authors: Bijandi Mehrdad , Karimi Mohammad , Farhadi Bansouleh Bahman , van der Knaap Wim 
Published: October 2020

AbstractIn the process of agricultural land consolidation, the land parcels are optimally redesigned and rearranged in such a way that the dimensions of the resulting parcels are proportional to agricultural criteria such as irrigation discharge, soil texture, and cropping pattern. Besides these criteria, spatial factors like slope, road accessibility, volume of earthwork, and geometrical factors such as size and shape of parcels are also included in the design process of agricultural land partitioning. In this study, a land partitioning model was proposed using a multi‐objective artificial bee colony algorithm (MOABC‐LP) taking into consideration the mentioned factors. Initially, a feasible dimension range of parcels in a block was calculated based on irrigation efficiency. Two partitioning layouts were defined according to the topography and geometry of blocks. The proposed method was applied to a real study area and the results suggest that the land partitioning plan obtained by the MOABC‐LP model, in comparison with a designer's plan, not only makes the shape and size of parcels more compatible with the topographical and agricultural conditions of each block, but also reduces their cut‐and‐fill ratio.