bluuebunny/crossref_metadata_2025_split huggingface dataset:
The heavy metal of cuprum (Cu) and lead(Pb) content in Avicennia marina and Rhizophora mucranata
Authors: Yunasfi  , Desrita  , Singh K P 
Published: November 2019

Abstract
Various types of mangrove plants found in coastal areas have a specific strategy to deal with pollution conditions from heavy metals. One of the several strategies used by mangroves is by accumulating various kinds of heavy metals in various parts of the tree. Most species of mangroves such as Avicennia marina, Rhizophora mucronata, Sonneratia caseolaris, Bruguiera gymnorhyza and others apply this strategy. Avicennia marina and Rhizophora mucronata are pioneer mangrove species that are able to accumulate heavy metals in the roots, stems and leaves. These species are also act as indicator of the sustainability of mangrove ecosystems. The presence of heavy metals will reduce water quality subsequently will decline the overall condition of the ecosystem. How big is the ability of Avicenia marina and Rhizopohora mucronata mangroves to absorb copper (Cu) and Lead (Pb) metals and how the conditions of the aquatic environment surrounding mangroves during heavy metal stress are need to be studied. Analysis of Cu and Pb heavy metals contents were carried out at the Chemistry Laboratory of the USU Medan Pharmacy Faculty. The mangrove species used for the analysis of Cu and Pb heavy metals were A.marina and R. mucronata that grew in Muara Sungai coastal areas from April to August 2018. The results showed heavy metal content of Cu was greater than Pb in wire roots, stems and leaves of A. marina trees. Analysis of roots, leaves and bark of R. mucronata also found greater content of Cu heavy metals than Pb.
Establishing Institution-Specific Normative Data for the BREAST-Q Reconstruction Module: A Prospective Study
Authors: Klifto Kevin M , Aravind Pathik , Major Melissa , Payne Rachael M , Shen Wen , Rosson Gedge D , Cooney Carisa M , Manahan Michele A 
Published: November 2019

Abstract

Background
Breast satisfaction in general female populations is relatively unknown and prior research populations do not reflect our community.

Objectives
We sought to assess breast satisfaction in a cohort of female participants utilizing the BREAST-Q and determine the impact of participant-related factors.

Methods
Females with no history of breast cancer or breast surgery attending gynecology appointments completed preoperative BREAST-Q reconstruction modules and demographic forms in this prospective, single-center, patient-reported outcomes study. We also assessed participant-related factors capable of influencing BREAST-Q scores.

Results
Three hundred females were included. Increasing body mass index had significant associations with lower Satisfaction with Breasts and Psychosocial Well-being scores. Increasing age was associated with significantly lower Sexual Well-being scores. African Americans had significantly higher scores for Satisfaction with Breasts, Psychosocial Well-being, and Sexual Well-being compared with Caucasians. Bra cup sizes A and C were associated with significantly higher Psychosocial Well-being scores than other sizes. Bra cup sizes A, B, and C were associated with significantly higher Sexual Well-being and Physical Well-being: Chest scores than larger sizes. Bra cup sizes B and C were associated with significantly higher Physical Well-being: Abdomen scores than size DD. Bra cup size A was associated with significantly higher Satisfaction with Breasts scores than sizes DD and &gt;DD. Bra cup size C was associated with significantly higher Satisfaction with Breasts scores than larger sizes.

Conclusions
Body mass index, age, race, and bra cup sizes significantly impact BREAST-Q scores in our population. Determining normative BREAST-Q scores in female populations could represent important baselines for breast outcomes research.

Level of Evidence: 2
Cell-Free Fat Extract Increases Dermal Thickness by Enhancing Angiogenesis and Extracellular Matrix Production in Nude Mice
Authors: Xu Yuda , Deng Mingwu , Cai Yizuo , Zheng Hongjie , Wang Xiangsheng , Yu Ziyou , Zhang Wenjie , Li Wei 
Published: November 2019

Abstract

Background
Although adipose-derived stem cells (ADSCs) and nanofat exert antiaging effects on skin, they contain cellular components that have certain limitations in clinical practice. Cell-free fat extract (Ceffe) is a fraction purified from nanofat through removal of cellular components and lipid remnants that contains various growth factors.

Objectives
The purpose of this study was to evaluate the effects of Ceffe on cultured human dermal fibroblasts in vitro and on the dermis of nude mice in vivo.

Methods
In the in vitro study, human dermal fibroblasts were cultured with Ceffe for 72 hours, followed by flow cytometry measurement of cell proliferation and cell cycle. In the in vivo study, different concentrations of Ceffe were injected into the dorsal skin of nude mice for 4 weeks. The thickness of the dermis; proliferation of cells; density of the capillary; and expressions of type I and III collagen (Col-1 and Col-3), matrix metalloproteinase-1, matrix metalloproteinase-3, tissue inhibitor of metalloproteinase-1, and tissue inhibitor of metalloproteinase-3 were measured through histologic and Western blot analyses.

Results
Ceffe significantly increased cell proliferation in cultured dermal fibroblasts. In the mouse skin, Ceffe significantly increased the thickness of the dermis, number of proliferating cells, density of the capillary, and expressions of Col-1 and Col-3.

Conclusions
Ceffe increased the dermal thickness of nude mice, possibly by enhancing angiogenesis and extracellular matrix production, and can therefore be used for skin rejuvenation.
Outcomes and Outcome Measures in Breast Reduction Mammaplasty: A Systematic Review
Authors: Waltho Daniel , Gallo Lucas , Gallo Matteo , Murphy Jessica , Copeland Andrea , Mowakket Sadek , Moltaji Syena , Baxter Charmaine , Karpinski Marta , Thoma Achilleas 
Published: November 2019

Abstract

Background
Reduction mammaplasty remains critical to the treatment of breast hypertrophy. No technique has been shown to be superior; however, comparison between studies is difficult due to variation in outcome reporting.

Objectives
The authors sought to identify a comprehensive list of outcomes and outcome measures in reduction mammaplasty.

Methods
A comprehensive computerized search was performed. Included studies were randomized or nonrandomized controlled trials involving at least 100 cases of female breast hypertrophy and patients of all ages who underwent 1 or more defined reduction mammaplasty technique. Outcomes and outcome measures were extracted and tabulated.

Results
A total 106 articles were eligible for inclusion; 57 unique outcomes and 16 outcome measures were identified. Frequency of patient-reported and author-reported outcomes were 44% and 88%, respectively. Postoperative complications were the most frequently reported outcome (82.2%). Quality-of-life outcomes were accounted for in 37.7% of studies. Outcome measures were either condition-specific or generic; frequencies were as low as 1% and as high as 5.6%. Five scales were formally assessed in the breast reduction populations. Clinical measures were defined in 15.1% of studies.

Conclusions
There is marked heterogeneity in reporting of outcomes and outcome measures in the literature. A standardized outcome set is needed to compare outcomes of various reduction mammaplasty techniques.

Level of Evidence: 4
A broad survey of DNA sequence data simulation tools
Authors: Alosaimi Shatha , Bandiang Armand , van Biljon Noelle , Awany Denis , Thami Prisca K , Tchamga Milaine S S , Kiran Anmol , Messaoud Olfa , Hassan Radia Ismaeel Mohammed , Mugo Jacquiline , Ahmed Azza , Bope Christian D , Allali Imane , Mazandu Gaston K , Mulder Nicola J , Chimusa Emile R 
Published: November 2019

AbstractIn silico DNA sequence generation is a powerful technology to evaluate and validate bioinformatics tools, and accordingly more than 35 DNA sequence simulation tools have been developed. With such a diverse array of tools to choose from, an important question is: Which tool should be used for a desired outcome? This question is largely unanswered as documentation for many of these DNA simulation tools is sparse. To address this, we performed a review of DNA sequence simulation tools developed to date and evaluated 20 state-of-art DNA sequence simulation tools on their ability to produce accurate reads based on their implemented sequence error model. We provide a succinct description of each tool and suggest which tool is most appropriate for the given different scenarios. Given the multitude of similar yet non-identical tools, researchers can use this review as a guide to inform their choice of DNA sequence simulation tool. This paves the way towards assessing existing tools in a unified framework, as well as enabling different simulation scenario analysis within the same framework.
Statistical methods for biomarker data pooled from multiple nested case–control studies
Authors: Sloan Abigail , Smith-Warner Stephanie A , Ziegler Regina G , Wang Molin 
Published: November 2019

Summary
Pooling biomarker data across multiple studies allows for examination of a wider exposure range than generally possible in individual studies, evaluation of population subgroups and disease subtypes with more statistical power, and more precise estimation of biomarker-disease associations. However, circulating biomarker measurements often require calibration to a single reference assay prior to pooling due to assay and laboratory variability across studies. We propose several methods for calibrating and combining biomarker data from nested case–control studies when reference assay data are obtained from a subset of controls in each contributing study. Specifically, we describe a two-stage calibration method and two aggregated calibration methods, named the internalized and full calibration methods, to evaluate the main effect of the biomarker exposure on disease risk and whether that association is modified by a potential covariate. The internalized method uses the reference laboratory measurement in the analysis when available and otherwise uses the estimated value derived from calibration models. The full calibration method uses calibrated biomarker measurements for all subjects, including those with reference laboratory measurements. Under the two-stage method, investigators complete study-specific analyses in the first stage followed by meta-analysis in the second stage. Our results demonstrate that the full calibration method is the preferred aggregated approach to minimize bias in point estimates. We also observe that the two-stage and full calibration methods provide similar effect and variance estimates but that their variance estimates are slightly larger than those from the internalized approach. As an illustrative example, we apply the three methods in a pooling project of nested case–control studies to evaluate (i) the association between circulating vitamin D levels and risk of stroke and (ii) how body mass index modifies the association between circulating vitamin D levels and risk of cardiovascular disease.
Societal Impact as ‘Rituals of Verification’ and The Co-Production of Knowledge
Authors: Crawford Adam 
Published: November 2019

Abstract
Thinking about and operationalizing societal impacts have become defining characteristics of university-based research, especially in the United Kingdom. This paper reflects on this unfolding shift in the conceptualization and practice of research with particular regard to criminology. It traces the development of new regulatory regimes that seek to measure research performance and render impact auditable. It argues that these ‘rituals of verification’ engender instrumental and narrow interpretations of impact that accord less space to research-informed social change as a non-linear and uncertain endeavour. This is juxtaposed with a conception of societal impact rooted in methodologies of co-production. Insights from the UK Research Excellence Framework 2014 and 2021 inform discussions and are contrasted with collaborative research efforts to apply co-production in policing research.
NeoPeptide: an immunoinformatic database of T-cell-defined neoantigens
Authors: Zhou Wei-Jun , Qu Zhi , Song Chao-Yang , Sun Yang , Lai An-Li , Luo Ma-Yao , Ying Yu-Zhe , Meng Hu , Liang Zhao , He Yan-Jie , Li Yu-Hua , Liu Jian 
Published: November 2019

AbstractTherapeutic vaccines represent a promising immunotherapeutic modality against cancer. Discovery and validation of antigens is the key to develop effective anti-cancer vaccines. Neoantigens, arising from somatic mutations in individual cancers, are considered as ideal cancer vaccine targets because of their immunogenicity and lack of expression in normal tissues. However, only few databases support convenient access to these neoantigens for use in vaccines. To address this gap, we developed a web-accessible database, called NeoPeptide, which contains most of the important characteristics of neoantigens (such as mutation site, subunit sequence, major histocompatibility complex restriction) derived from published literature and other immunological resources. NeoPeptide also provides links to resources for further characterization of the novel features of these neoantigens. NeoPeptide will be regularly updated with newly identified and published neoantigens. Our work will help researchers in identifying neoantigens in different cancers and hasten the search for appropriate cancer vaccine candidates.
Type of Disaster Exposure Affects Functional Limitations of Older People 6 Years Later
Authors: Pruchno Rachel , Wilson-Genderson Maureen , Heid Allison R , Cartwright Francine P 
Published: November 2019

Abstract

Background
Natural disasters, including earthquakes, tsunamis, tornadoes, and hurricanes, are traumatic events that simultaneously affect the lives of many people. Although much is known about the effects that natural disasters have on mental health, little is known about how natural disasters affect physical health. These analyses add to the literature by examining the ways in which four types of disaster exposure (geographic, peri-traumatic stress, personal and property loss, and poststorm hardship) experienced by older people during and after Hurricane Sandy affected functional limitations.

Methods
We analyzed five waves of data from the ORANJ BOWL panel (“Ongoing Research on Aging in New Jersey: Bettering Opportunities for Wellness in Life”) using multilevel mixed-effects models.

Results
We found that although peri-traumatic stress and poststorm hardship each had independent effects on functional limitations, the effects of peri-traumatic stress dominated and were evident 6 years after the hurricane. Geographic exposure and personal/property loss were not associated with functional limitations.

Conclusions
These findings add important information to what is known about older people who experience a natural disaster and suggest opportunities for intervention. Finding that an individual’s emotional response during the disaster plays an important role in the development of functional limitations suggests that reduction of exposure to traumatic stress during a storm (ie, evacuation from a storm area) may be important for older people. Likewise, interventions immediately after a disaster that target older people who experience high levels of peri-traumatic distress may be needed in order to alleviate functional limitations before they develop.
Vaccinations and Immunization Status in Pediatric Inflammatory Bowel Disease: A Multicenter Study From the Pediatric IBD Porto Group of the ESPGHAN
Authors: Martinelli Massimo , Giugliano Francesca Paola , Strisciuglio Caterina , Urbonas Vaidotas , Serban Daniela Elena , Banaszkiewicz Aleksandra , Assa Amit , Hojsak Iva , Lerchova Tereza , Navas-López Víctor Manuel , Romano Claudio , Sladek Małgorzata , Veres Gabor , Aloi Marina , Kucinskiene Ruta , Miele Erasmo 
Published: November 2019

AbstractBackgroundVaccine-preventable diseases and opportunistic infections in pediatric inflammatory bowel disease (IBD) are increasingly recognized issues. The aims of this study were to evaluate vaccinations, immunization status, and consequent therapeutic management in children with IBD and to analyze the differences among patients diagnosed before (Group 1) and after June 2012 (Group 2).MethodsThis was a multicenter, retrospective cohort investigation. Between July 2016 and July 2017, 430 children with IBD were enrolled in 13 centers. Diagnosis, therapeutic history, vaccinations, and immunization status screening at diagnosis and at immunosuppressant (IM)/biologic initiation and reasons for incomplete immunization were retrieved.ResultsVaccination rates at diagnosis were unsatisfactory for measles, mumps, and rubella (89.3%), Haemophilus influenzae (81.9%), meningococcus C (23.5%), chickenpox (18.4%), pneumococcus (18.6%), papillomavirus (5.9%), and rotavirus (1.9%). Complete immunization was recorded in 38/430 (8.8%) children, but specific vaccines were recommended in 79/430 patients (18.6%), without differences between the 2 groups. At IM start, 22% of children were tested for Epstein-Barr virus (EBV) status, with 96.2% of EBV-naïve patients starting azathioprine, without differences between Groups 1 and 2. Screening for latent tuberculosis (TB) before start of biologics was performed in 175/190 (92.1%), with up to 9 different screening strategies and numerous inconsistencies.ConclusionsWe demonstrated a poor immunization status at diagnosis in children with IBD, which was not followed by proper vaccination catch-up. EBV status before IM initiation and latent TB before biologics were not adequately assessed. Thus, the overall impact of the current guidelines seems unsatisfactory.
Effects of vitamin B6 on growth, diarrhea rate, intestinal morphology, function, and inflammatory factors expression in a high-protein diet fed to weaned piglets1
Authors: Li Jun , Yin Lanmei , Wang Lei , Li Jianzhong , Huang Pengfei , Yang Huansheng , Yin Yulong 
Published: November 2019

Abstract
Vitamin B6 (VB6) is an important coenzyme factor which participates in many metabolic reactions, especially amino acid metabolism. There are few reports on how VB6 mediates weaned piglet intestinal health. This study purposed to investigate dietary VB6 effects on growth, diarrhea rates, and intestinal morphology and function in weaned piglets fed a high-crude protein (22% CP) diet. Eighteen 21-d-old weaned [(Yorkshire × Landrace) × Duroc] piglets with body weights of 7.03 ± 0.15 (means ± SEM) kg were randomly assigned into 3 VB6-containing dietary treatments. Vitamin B6 content was: 0, 4, and 7 mg/kg, respectively. The feeding period lasted 14 d. The results showed that no significant difference existed for the growth performance. The 7 mg/kg VB6 group had a tendency to decrease diarrhea rate (P = 0.065). Blood biochemical parameters analysis demonstrated that total protein, cholesterol, and high-density lipoprotein significantly increased in the 7 mg/kg VB6 group (P &lt; 0.05). In the jejunum, no significant differences were detected for villus height, villus width, crypt depth, villus height and crypt depth ratios, and positive Ki67 counts and the mRNA expression of inflammatory cytokines. Vitamin B6 significantly increased the mRNA expression of SLC6A19 and SLC6A20 (P &lt; 0.05) and decreased the mRNA expression of SLC36A1 (P &lt; 0.05). In the ileum, VB6 significantly increased villus height and villus width (P &lt; 0.05) while decreased positive Ki67 cell counts for 7 mg/kg VB6 group (P &lt; 0.05). Vitamin B6 had significantly increased the mRNA expression of interleukin-1β, tumor necrosis factor-α,cyclo-oxygen-ase-2, and transforming growth factor-β (P &lt; 0.05). Vitamin B6 also had significantly increased mRNA expression of SLC6A19, SLC7A6, SLC7A7, and SLC36A1 (P &lt; 0.05). These findings suggest that dietary supplementation with VB6 may affect the intestinal morphology and absorption and metabolism of protein in weaned piglets fed a high-protein diet by altering the expression of intestinal inflammatory cytokines and amino acid transporters.
Dietary supplementation with free methionine or methionine dipeptide improves environment intestinal of broilers challenged with Eimeria spp.
Authors: Khatlab Angélica de Souza , Del Vesco Ana Paula , Rodrigues Oliveira Neto Adhemar , Almeida Fernanda Losi Alves , Gasparino Eliane 
Published: November 2019

Abstract
This study examined the influence of a diet enriched with free methionine (dl-Met) or methionine dipeptide (dl-MMet) on the intestinal health of Eimeria-challenged (EC) and unchallenged (UC) broilers. A non-supplemented, methionine-deficient diet (NS) was used as control. Treatments were arranged in a 2 × 3 factorial completely randomized design with eight replications. Broilers in the EC group were infected with sporulated oocysts of Eimeria spp. (E. acervulina, E. maxima, E. praecox, and E. mitis) at 14 d of age. Performance analysis, light and electron microscopy of the jejunum, analysis of genes related to apoptosis and cell proliferation in the jejunum, and blood tests were performed at 6 days post-inoculation (dpi). EC broilers had poorer performance than UC broilers, regardless of diet (P &lt; 0.001). Broilers fed the dl-Met diet had greater weight gain (P = 0.004) and lower feed conversion ratio (P = 0.019) than broilers fed other diets. Jejunal sections from EC broilers fed the NS diet showed short (P = 0.001) and wide villi (P &lt; 0.001) with increased crypt depth (P &lt; 0.001) and reduced villus / crypt ratio (P = 0.001), jejunal absorptive surface area (P &lt; 0.001), number of neutral goblet cells (Eimeria challenge: P = 0.048; diet P = 0.016), and mucin 2 (MUC2) gene expression (P = 0.018). EC birds fed the dl-MMet diet had higher enterocyte height (P &lt; 0.001). Birds fed the dl-MMet diet had low lamina propria width (P = 0.009). UC broilers fed the dl-Met diet had the highest number of acidic goblet cells (P = 0.005), whereas EC broilers assigned the dl-MMet diet showed the highest number of intraepithelial lymphocytes (P = 0.033). Reduced expression of caspase-3 (CASP3) (P = 0.005), B-cell lymphoma 2 (BCL2) (P &lt; 0.001), mechanistic target of rapamycin (MTOR) (P &lt; 0.001), and ribosomal protein S6 kinase B1 (RPS6KB1) (P &lt; 0.001) genes was observed in EC animals. MTOR expression levels were highest in birds fed the dl-MMet diet (P = 0.004). Plasma activities of aspartate aminotransferase (AST) was influenced by both diet (P = 0.002) and Eimeria challenge (P = 0.005), with EC broilers assigned the NS diet showing the highest levels. EC broilers fed the NS diet had higher creatine kinase (CK) activity (P = 0.049). EC broilers had lower plasma uric acid (P = 0.004) and higher serum mucoproteins level (P &lt; 0.001). These results indicate that methionine dipeptide supplementation is able to mitigate the harmful intestinal effects of Eimeria spp. in broilers.