bluuebunny/crossref_metadata_2025_split huggingface dataset:
Medium- and Long-Term Effects of Endovascular Treatments for Severely Stenotic Basilar Arteries Supported by Multimodal Imaging
Authors: Li Yuanzhi , Xie Weimin , Gong Xianghao , He Dongliang , Zhang Xin , Song Ligang , Li Zhenfa 
Published: May 2020

Abstract
Background: To evaluate the medium-and long-term effect of intravascular interventional therapy for symptomatic severe basilar artery stenosis supported by multimodal imaging.Method: After strict screening of 67 patients with symptomatic severe basilar artery stenosis (70%- 99%) with atherosclerotic stenosis, 67 patients with symptomatic recurrence after intensive drug treatment were treated with intravascular balloon dilatation and Enterprise stent implantation. Any stroke or death within 30 days after operation and any stroke and restenosis during medium-and long-term follow-up were followed up.Results: The mean age of 67 patients(67lesions) was 57 ± 8 years old, and the technical success rate was 100%; ②Preoperative angiography showed that the collateral circulation was poor, and TICI was 1-2a while postoperative angiography showed that TICI was significantly improved to 2b-3; ③The average preoperative stenosis rate was 82 ± 9%, and the postoperative stenosis rate was reduced to 17 ± 10%; ④Before surgery, abnormal perfusion was found in the posterior circulation CTP; After the postoperative re-examination, the posterior circulation of CTP perfusion was significantly improved; ⑤Postoperative symptoms and neurological conditions improved significantly; ⑥Complications of perforating branch event occurred in 1 case after operation, and symptoms were relieved after more than 1 month of active treatment, and mild neurological dysfunction remained. 1 case developed subacute thrombosis in the stent, which improved after active intra-arterial thrombolysis, and there was no residual neurological dysfunction; and 1 case of microlead wire being clamped by the distal vasospasm. ⑦67 patients were followed up by telephone, WeChat or imaging for 36–66 months. 55 cases were regularly reviewed with images. Intrastenting stenosis occurred in 4 cases, and 12 cases were followed up by phone or WeChat.The average follow-up period was 56 ± 21 months (36–66 months). No TIA or stroke occurred during follow-up.Conclusions: In summary intravascular balloon dilation + Enterprise stent implantation is safe and effective for the treatment of symptomatic severe atherosclerotic stenosis of the basilar artery, with high technical success rate, low perioperative complications, and good mid-term and long-term effects.
Synthetic Repurposing of Drugs in Hypertension: a Datamining Method Based on Association Rules and a Novel Discrete Algorithm
Authors: Masoudi-Sobhanzadeh Yosef , Masoudi-Nejad Ali 
Published: May 2020

Abstract
Background Drug repurposing aims to detect the new benefits of the existing drugs and to reduce the spent time and cost of the drug development projects. Although synthetic repurposing of drugs may be more useful than single repurposing in terms of reducing toxicity and enhancing efficacy, the researchers have not taken it into account. To address the issue, a novel datamining method is introduced and applied to the repositioning of drugs in hypertension (HT), which is a serious medical condition and therefore needs to be dealt with effectively through making some improved treatment plans to help cure it. Results a novel two-step data mining method, which is based on the If-Then association rules and a novel discrete optimization algorithm, is proposed and applied to the synthetic repurposing of drugs in HT. The required data are extracted from DruhBank, KEGG, and DrugR+ databases. The outcomes presented that the proposed method outperforms the other state-of-the-art approaches in terms of different statistical criteria. In contrast to the previously proposed methods which failed to discover a list for some datasets, our method managed to suggest a combination of drugs for all the datasets. Conclusion The proposed synthetic method may revive some failed drug development projects and be a suitable plan for curing orphan and rare diseases due to using a low dosage of medicines. It is also essential to use some efficient computational methods to produce better results.
Performance of a novel dialyzer containing a fluorinated polyurethane surface-modifying macromolecule in patients with end-stage kidney disease
Authors: Meyer Jill M , Steer Dylan , Weber Lisa A , Zeitone Abeer A , Thakuria Mayuri , Ho Chiang-Hong , Aslam Shakil , Mullon Claudy , Kossmann Robert J 
Published: May 2020

Abstract
Background By inhibiting the adsorption of protein and platelets, surface-modifying macromolecules (SMMs) may improve the hemocompatibility of hemodialyzers. This trial aims to assess the performance and safety of a novel dialyzer with a fluorinated polyurethane SMM, Endexo™, blended into the membrane during manufacturing. Methods This prospective, sequential, multicenter, open-label study enrolled adults prescribed thrice-weekly hemodialysis. After completing 12 hemodialysis sessions with an Optiflux ® F160NR dialyzer , patients received 38 sessions with the dialyzer with Endexo. Evaluated parameters included the extent of removal of urea, albumin, and β2-microglobulin (β2M), as well as complement activation.Results Twenty-three patients received 268 hemodialysis treatments during the Optiflux period, and 18 patients continued on to receive 664 hemodialysis treatments during the Endexo period. Mean treatment times (208 vs 205 min), blood flow rates (447.7 vs 447.5 mL/min), dialysate flow rates (698.5 vs 698.0 mL/min), urea reduction ratio (82% vs 81%) and spKt/V (2.1 vs 1.9) were comparable for the Endexo and Optiflux periods, respectively. No overt complement activation was observed. Both dialyzers were associated with comparable mean increases in serum albumin levels from pre- to post- hemodialysis (Optiflux: 7.9%; Endexo: 8.0%). The corrected mean β2M removal rate was 47% higher during the Endexo period (67.73%). Three serious adverse events were reported, none of them device-related. Conclusions The performance of the novel dialyzer with Endexo was generally comparable to the Optiflux dialyzer, while exhibiting a higher β2M removal rate. Additional studies are needed to determine whether this novel dialyzer can be incorporated into heparin-free hemodialysis approaches.
Study on community structure and function of symbiotic bacteria at different growth and developmental stages of Hypsizygus marmoreus
Authors: sun shujing , Li Fan , Xu Xin , Liu Yunchao , Kong Xuqiang , Chen Jianqiu , Liu Ting , Chen Liding 
Published: May 2020

Abstract
Background
The symbiotic bacteria occurring naturally in the cultivation bags of edible fungi during the mycelial growth is a huge resource to be explored. It is of significance to analyze the community structure and succession of symbiotic bacteria at the different growth and developmental stages of edible fungi and isolate growth-promoting strains that have an essential relationship with cultivation cycle, and traits and yields of fruiting bodies.
Results
In all samples from cultivation bags of Hypsizygus marmoreus, 34 bacterial phyla were detected, and Firmicutes was the most abundant bacterial phylum (78.85%). Serratia genera increased exponentially in cultivation bags in the matured period reaching the peak abundance of 55.74%, and became the dominant symbiotic flora. The most eye-catching strain was Serratia odorifera HZSO-1, and its quantity increased with the amount of hyphae of H. marmoreus. The results also verified that S. odorifera HZSO-1 was a symbiotic bacterium residing in the hyphae of H. marmoreus, and can promote the growth and development of H. marmoreus, shorten the fruiting cycle by 3–4 days, and increase the fruiting body yield by 12%.
Conclusions
This study was a pioneering demonstration of the community structure of symbiotic microbiota and its potential micro-ecology function in the growth and development of edible fungi and will lay a theoretical foundation of bacteria-mushroom interaction.
Development of a Double-Recombinant Antibody Sandwich ELISA for Quantitative Detection of Epsilon Toxoid Concentration in Inactivated Clostridium Perfringens Vaccines
Authors: Alibeiki Maryam , Golchin Mehdi , Tabatabaei Mohammad 
Published: May 2020

Abstract
Background: Epsilon toxin (ETX) causes a commonly fatal enterotoxemia in domestic animals and causing serious economic losses to animal husbandry. In this study, we selected several clones against ETX using repertoires displayed on filamentous phage. Anti-ETX specific clones were enriched by binding to immobilized antigen, followed by elution and re-propagation of phage. After multiple rounds of binding selection, we evidenced that most isolated clones had high affinity and specificity for the ETX by ELISA.Results: We isolated two recombinant monoclonal antibodies against ETX by phage display technology and Using the B1 phage VH antibody isolated from DAb library as capture antibody and G2 soluble scFv antibody isolated from Tomlinson I + J libraries as the detector antibody, we developed a sandwich ELISA test.Conclusions: This sandwich ELISA could be a good candidate for quantitative detection ETX with the detection range of 5 ~ 100000 ng/ml in inactivated commercial vaccines against enterotoxemia.
Big Data Analysis of Pulmonary Embolism in Greece
Authors: Lampropoulos Ioannis Ch , Raptis Dimitrios G , Daniil Zoe , Tasoulis Sotirios K , Plagianakos Vassilis P , Malli Foteini , Gourgoulianis Konstantinos I 
Published: May 2020

Abstract
Background: Pulmonary embolism (PE) epidemiological data are unclear considering the disease prevalence in the general population. The present study aims to investigate the prevalence of PE at the Greek population and the associated trends for the years 2013-2017. Methods: Data on prescriptions for PE in the years 2013-2017 were provided by the Greek National Health Service Organization (EOPYY). Data on age, gender, specialty of the prescribing physician and prescription unit were provided as well. Results: The total number of prescriptions for PE for the study period was 101.426. Of the total prescriptions, 51% were issued by the Public Sector, while 48% by the Private Sector. In 2013 the prevalence of PE was 5.43 cases per 100.000 citizens and increased constantly until 2017 with 23.79 cases per 100.000 population. Prevalence is higher in all years studied in the age group 70-80 with 69.35 cases per 100.000 population for the year 2017 followed by the ages 80-90 (60.58/100.000) and 60-70 (56.47/100.000) for the same year. Females display higher PE prevalence than males and higher increasing trend.Conclusion: PE prevalence displays an increasing trend throughout the years while female prevalence is higher than males with a higher increasing trend. Our results may be used to appropriately organize nationwide health care systems aiming at the diagnosis, treatment and prevention of PE.
Bitcoin and Stock Markets: Are They Connected? Evidence from Asean Emerging Economies
Authors: Mand Abdollah Ah , Thaker Hassanudin Mohd Thas 
Published: May 2020

Abstract
Background: Cryptocurrencies, especially Bitcoin, has become popular for investors in recent years. The volatility of bitcoin and time horizon are the center point for investment decisions. However, attention is not often drawn to the relationship between bitcoin and equity indices. This study investigates the volatility and time frequency domain of bitcoin among five Asean countries through a rich database which covers daily data from July 2010 until April 2019.Methods: Advanced econometrics and Wavelets Cross-Coherence Spectrograms, this study investigates the existence of long run association between bitcoin and the studied market indices. M-GARCH analysis is been employed to investigate the unconditional volatility of market indices and Bitcoin.Results: The findings present the long run association with positive (Philippines) and negative (Japan, Korea, Singapore, Hong Kong) relations. Moreover, only one market (KOREA) shows a short run association with bitcoin. The M-GARCH analysis reveals, most of the selected Asean countries have a low unconditional volatility with bitcoin. Except for Philippines in which the co-movement is average, Wavelet analysis reveals the presence of a strong and long co-movements for most of the selected Asean countries with bitcoin.Conclusions: Most of our results are consistent and illustrate different dimensions of long and short run relationship, volatilities, correlations, and time-frequency analysis. This study utilized Asean emerging economies which are rarely available in the literature as existing studies are more skewed towards the West. We believe the outcomes of this study will be a significant for industry practitioners (i.e., retail and institutional investors) on designing better strategies to diversify the stock portfolio with different holding period horizons and dimensions.
“Oil-soluble” Reversed Lipid Nanoparticles for Oral Insulin Delivery
Authors: Wang Tao , Quan Dongqin 
Published: May 2020

Abstract
Background
In this study, we aimed to design a novel oral insulin delivery system, named “oil-soluble” reversed lipid nanoparticles (ORLN), in which a hydrophilic insulin molecule is encapsulated by a phospholipid (PC) shell and dissolved in oil to prevent the enzymatic degradation of insulin. ORLN was characterized by transmission electron microscopy and dynamic light scattering.
Results
In vitro enzymatic stability studies showed higher concentrations of insulin in cells incubated with ORLN-encapsulated insulin than in those incubated with free insulin solution in artificial intestinal fluid (pH 6.5). The protective effect of ORLN was attributed to its special release behavior and the formulation of the PC shell and oil barrier. Furthermore, an in vivo oral efficacy study confirmed that blood glucose levels were markedly decreased after ORLN administration in both healthy and diabetic mice. In vivo pharmacokinetic results showed that the bioavailability of ORLN-conjugated insulin was approximately 28.7% relative to that of the group subcutaneously administered with an aqueous solution of insulin, indicating enhanced oral absorption.
Conclusions
In summary, the ORLN system developed here shows promise as a nanocarrier for improving the oral absorption of insulin.
Understanding the Regulatory Mechanisms of Endometrial Cells on Activities of Endometrial Mesenchymal Stem-Like Cells During Menstruation
Authors: Xu Shan , Chan Rachel W.S. , Li Tianqi , Ng Ernest H.Y. , Yeung William S.B. 
Published: May 2020

Abstract
Background
The identification of endometrial stem/progenitor cells in a high turnover rate tissue suggests that a well-orchestrated underlying network controls the behaviour of these stem cells. The thickness of the endometrium can grow from 0.5–1 mm to 5–7 mm within a week indicating the need of stem cells for self-renewal and differentiation during this period. The cyclical regeneration of the endometrium suggests specific signals can activate the stem cells during or shortly after menstruation.
Methods
Endometrial mesenchymal stem-like cells (eMSCs) were cocultured with endometrial epithelial or stromal cells from different phases of the menstrual cycle, the clonogenicity and the phenotypic expression of eMSC markers (CD140b and CD146) were assessed. The functional role of WNT/β-catenin signalling on eMSC was determined by Western blot analysis, immunofluorecent staining, flow cytometry, quantitative real-time PCR and small interfering RNA. The cytokine levels in the conditioned medium of epithelial or stromal cells cocultured with eMSCs was evaluated by ELISA.
Results
Coculture of endometrial cells (epithelial or stromal) from the menstrual phase enhanced the clonogenicity and self-renewal activities of eMSCs. Such phenomenon was not observed in niche cells from the proliferative phase. Coculture with endometrial cells from the menstrual phase confirmed an increase in expression of active β-catenin in the eMSCs. Treatment with IWP-2, a WNT inhibitor suppressed the observed effects. Anti-RSPO1 antibody reduced the stimulatory action of endometrial niche cells on WNT/β-catenin activation in TCF/LEF luciferase reporter assay. Moreover, the mRNA level and protein immunoreactivities of LGR5 were higher in eMSCs than unfractionated stromal cells. Conditioned media of endometrial niche cells cocultured with eMSCs contained increased levels of C-X-C motif ligand 1 (CXCL1), CXCL5 and interleukin-6. Treatment with these cytokines increased the clonogenic activity and phenotypic expression of eMSCs.
Conclusions
Our findings indicate a role of WNT/β-catenin signalling in regulating activities of endometrial stem/progenitor cells during menstruation. Certain cytokines at menstruation can stimulate the proliferation and self-renewal activities of eMSCs. Understanding the mechanism in the regulation of eMSCs may contribute to treatments of endometrial proliferative disorders such as Asherman’s Syndrome.
Primary and secondary aortopathy associated with adult congenital heart disease - retrospective study
Authors: Schusterova Ingrid , Banovcinova Alzbeta , Vachalcova Marianna , Jakubova Marta , Artemiou Panagiotis 
Published: May 2020

Abstract
Background: Primary and secondary aortopathy are frequently encountered in patients with congenital heart disease. The aim of this study is to present our experience and the incidence of primary and secondary adult CHD-associated aortopathy.Methods. The cohort is comprised of adult patients with congenital heart disease from the registry of the Eastern Slovakia Institute of Cardiovascular Diseases. Data from the last follow-up examinations are included in this study. In the primary and secondary aortopathy group were 35 and 12 patients respectively. As a control group were selected 64 patients with non aortopathy associated congenital heart disease (atrial and ventricular septal defect).Results: Patients with primary and secondary aortopathy had larger ascending aorta/aortic root diameters than the control group (36.28 (26-49) mm vs 30.25 (21-41) mm p=0.000113, 33.82 27-49) mm vs 29.03 (19-38)mm p=0.000366 and 42.1 (30-50) mm vs 30.25 (21-41) mm, p=0.000106, 35.67 (27-48) mm vs 29.03 (19-38) mm, p=0.000119 respectively). Moreover, patients with secondary aortopathy had statistically significant larger ascending aorta diameter compared to the patients with primary aortopathy ( 42.1 (30-50) mm vs 36.28 ( 26-49) mm p=0.030). During the follow-up period, were performed only in 2 patients (one from each group) operations on the aortic root and the ascending aorta due to aortic root or ascending aorta dilatation.Conclusion: More patients with secondary aortopathy had dilated ascending aorta/ aortic root, as well as larger aortic diameters compare to the patients with primary aortopathy. Routine follow-up of these patients with attention to aortic diameter is necessary.
Thyroid Hormones and Antibodies: An Economic Risk Factor to Remember
Authors: Uncu Gülgün , Ayas Zeynep Özözen , Algın Demet İlhan , Aldemir Arzu , Adapınar Demet Özbabalık 
Published: May 2020

Abstract
BackgroundThyroid metabolites play an important role in cognitive functions. It is absolutely recommended to evaluate thyroid function tests (TFT) in the etiologic examination of cognitive disorders in dementia. In this study, we aimed to compare laboratory and cognitive data of patients aged under 65 years who presented to the other clinics with memory loss and who had been diagnosed with Alzheimer disease (AD).MethodsWe retrospectively reviewed the findings of patients aged under 65 years meeting the inclusion criteria of the study. Before admitting to our clinic, the patients who had memory problems were diagnosed with AD without screening TFT and started medications in different centers were screened. After performing thyroid screening for differential diagnosis in our first evaluation, patients who had diagnosed with disorders of thyroid hormones or/and antibodies and started to treat with thyroid medical therapy, included in our study.There were three groups. GroupI patients had elevated values of fT3 and anti-TPO, normal anti-TG levels. GroupII patients had decreased fT3 and fT4 levels and normal values of antibodies. GroupIII patients had normal fT3-fT4 and elevated values of anti-TPO and anti-TG. The patients of medications for AD (donepezil/rivastigmine/memantine/irregular medications) were recorded. The patients were followed-up for at 1st and 24th weeks after starting thyroid medical treatment. Follow-up of the patients was performed by the standardized mini mental state examination (MMSE), Verbal memory process, Boston naming test (BNT), Frontal assessment battery (FAB), Barthel activities of daily life index (BI) and Ideomotor apraxia test (IAT).ResultsWe identified 55 (30 female-25 male) patients who could be included. The mean age of patients was 51.8±7.6 (35-65). There were 40 (72.7%) patients in GroupI, 11 (20.2%) in GroupII, and 4 (7.1%) in GroupIII.MMSE, BNT, FAB (similarities go-no-go and lexical fluency), IAT were found statistically significant differences in all groups. Cognitive tests were found to be improved in patients. Although BI was found significant improvement in GroupII at 24th week. ConclusionIf there is a disorder of thyroid hormones and antibodies in patients with memory loss, the treatment of thyroid dysfunction should be planned before onset of anti-dementia medications.
Communality from equal weight for GIS-based landslide susceptibility mapping
Authors: Som Sandip , Ghosh Saibal , Dasgupta Soumitra , Kumar Thrideep , Hindayar J. N. , Mohan Murali , Dasarwar P. 
Published: May 2020

Abstract
Modeling landslide susceptibility is one of the important aspects of land use planning and risk management. Several modeling methods are available based either on highly specialized knowledge on causative attributes or on good landslide inventory data to use as training and testing attribute on model development. Understandably, these two criteria are rarely available for local land regulators. This paper presents a new model methodology, which requires minimum knowledge of causative attributes and does not depend on landslide inventory. As landslide causes due to the combined effect of causative attributes, this model utilizes communality (common variance) of the attributes, extracted by exploratory factor analysis and used for calculation of landslide susceptibility index. The model can understand the inter-relationship of different geo-environmental attributes responsible for landslide along with identification and prioritization of attributes on model performance to delineate non-performing attributes. Finally, the model performance is compared with the well established AHP method (knowledge driven) and FRM method (data driven) by cut-off independent ROC curves along with cost-effectiveness. The model shows it’s performance almost at par with the established models, involving minimum modeling expertise. The findings and results of the present work will be helpful for the town planners and engineers on a regional scale for generalized planning and assessment.