bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comprehensive analysis of allergen-specific IgE in COPD: Mite-specific IgE specifically related to the diagnosis of asthma-COPD overlap
Authors: Toyota Hikaru , Sugimoto Naoya , Kobayashi Konomi , Suzuki Yuki , Takeshita Yuri , Ito Ayaka , Ujino Mariko , Tomyo Fuminori , Sakasegawa Hirokazu , Koizumi Yuta , Kuramochi Michio , Yamaguchi Masao , Nagase Hiroyuki 
Published: August 2020

Abstract
Background
Although the relationship between allergic sensitization and increased respiratory symptoms of chronic obstructive pulmonary disease (COPD) has been suggested, which allergen has a significant effect on COPD pathology is unclear. This study aimed to identify the specific IgE related to clinical features of COPD and the diagnosis of asthma-COPD overlap (ACO).
Methods
We recruited 70 patients with COPD and analyzed 39 IgE using panel IgE test (View Allergy 39®). ACO was diagnosed according to the Japanese Respiratory Society Guidelines.
Results
As for perennial aeroallergens, the positivity for moth (35.4%), cockroach (23.1%), Dermatophagoides pteronyssinus (21.5%), house dust (21.5%), and Candida (21.5%) and, concerning pollen, Japanese cedar (36.9%) and Japanese cypress (23.1%) exceeded 20%. Only the positivity of IgE for Dermatophagoides pteronyssinus and house dust was significantly higher in ACO compared with that in non-ACO COPD. Moreover, it contributed to the diagnosis of ACO in an IgE class-dependent manner. Patients with cockroach IgE exhibited higher residual volume, whereas those with Japanese cedar IgE exhibited better diffusion capacity than negative patients. The contribution for ACO diagnosis by the receiver operating characteristic curve analysis was comparable among total IgE (cutoff value: 158 IU/mL), blood eosinophil count (234/µl), and fraction of exhaled nitric oxide (31.0 ppb).
Conclusions
The prominent role of mite-specific IgE in the diagnosis and pathology of ACO and the potentially detrimental effect of cockroach sensitization on air trapping in COPD were suggested. The finding highlights the future development of a treatment targeting IgE as a treatable trait in COPD.
Penicillium Janthinellum NCIM-1366 Shows Improved Biomass Hydrolysis Performance Over Trichoderma Reesei RUT-C30, and Secretome Analysis Reveals a Larger Number of CAZymes with Higher Induction Levels.
Authors: Sreeja-R AthiraRaj , Christopher Meera , Kooloth-Valappil Prajeesh , Kuni-Parambil Rajasree , Gokhale Digambar Vittal , Sankar Meena , Abraham Amith , Pandey Ashok , Sukumaran Rajeev Kumar 
Published: August 2020

Abstract
BackgroundMajor cost of bioethanol is attributed to enzymes employed in biomass hydrolysis. Lignocellulolytic enzymes are predominantly produced from the hyper cellulolytic mutant filamentous fungus Trichoderma reesei RUT-C30. Several decades of research have failed to provide an industrial grade organism producing higher titers of an effective synergistic biomass hydrolyzing enzyme cocktail. Penicillium janthinellum NCIM1366 was reported as a cellulase hyper producer and a potential alternative to T. reesei, but a comparison of their hydrolytic performance was seldom attempted. ResultsHydrolysis of acid or alkali pretreated rice straw using cellulase enzyme preparations from P. janthinellum and T. reesei indicated 37 and 43 % higher glucose release respectively with P. janthinellum enzymes. A comparison of these fungi with respect to their secreted enzymes indicated that the crude enzyme preparation from P. janthinellum showed 28 % higher overall cellulase activity. It also had an exceptional 10-fold higher beta-glucosidase activity compared to that of T. reesei, leading to a lower cellobiose accumulation and thus alleviating the feedback inhibition. P. janthinellum secreted more number of proteins to the extracellular medium whose total concentration was 1.8 fold higher than T. reesei. Secretome analyses of the two fungi revealed more number of CAZymes and a higher relative abundance of cellulases upon cellulose induction in the fungus.ConclusionsThe results revealed the ability P. janthinellum for efficient biomass degradation through hyper cellulase production, and it outperformed the established industrial cellulase producer T. reesei in the hydrolysis experiments. A higher level of induction, larger number of secreted CAZymes and a high relative proportion of BGL to cellulases could be the possible reasons for its performance advantage in biomass hydrolysis.
Relationship of the Inflammatory Diet Status and Dietary Intake with Antimalarial Treatment in Systemic Lupus Rrythematosus: A Cross-Sectional Study
Authors: Meza-Meza Mónica R. , Shivappa Nitin , Montoya-Buelna Margarita , Muñoz-Valle José Francisco , Hébert James R. , Vizmanos-Lamotte Barbara , Parra-Rojas Isela , Campos-López Bertha , Cerpa-Cruz Sergio , Cruz-Mosso Ulises De la 
Published: August 2020

Abstract
Background: Systemic lupus erythematosus (SLE) is the prototype autoimmune disease with high inflammatory cytokine levels. In autoimmune conditions, diet could modify the inflammatory status, comorbidities and pharmacotherapy administered in SLE patients. The aim of this study was to assess the relationship of the inflammatory diet status and dietary intake with comorbidities and pharmacotherapy administered in SLE patients. Methods: A cross-sectional study was conducted in sixty-eight Mexican-Mestizo female SLE patients. Dietary intake was estimated from three 24h food records by Nutritionist Pro-Diet software and 27 food parameters were used to evaluate the inflammatory diet status by the normal dietary inflammatory index (DII®). Results: SLE patients presented a global pro-inflammatory diet status (DII: 0.71 ± 1.78). Chloroquine (CQ) administration was related to a pro-inflammatory diet status compared to hydroxychloroquine (HCQ) administration, which was related to an anti-inflammatory diet status (CQ= DII score: 1.385±1.327 vs. HCQ= DII score: 0.004±2.024; p=0.002). CQ administration conferred a pro-inflammatory DII score (β coefficient= 1.20; CI: 1–2.02; R2=0.11; p<0.01) and lower total-cholesterol (β coefficient=-29.2; CI: -4.03 to -54.5; R2=0.07; p<0.05); conversely, HCQ administration conferred an anti-inflammatory DII score (β coefficient= -1.29; CI: -0.46 to -2.12; R2=0.13; p<0.01). SLE patients with CQ administration had lower intake of energy and 12 nutrients evaluated (CQ vs. HCQ; p <0.05), and SLE patients with HCQ treatment had a better achievement ≥100% of daily dietary reference intake (DRI) of energy (HCQ 77% vs. CQ 52%; p=0.03), vitamin A (HCQ 65% vs. CQ 29%; p<0.01), cholesterol (HCQ 29% vs. CQ 3%; p<0.01), and fiber (HCQ 26% vs. CQ 6%; p=0.03).Conclusions: In SLE patients the CQ administration was related to a pro-inflammatory diet status and low total-cholesterol, and HCQ administration with an anti-inflammatory diet status and better dietary intake.
Natural fluids collected from the reproductive tract of women can be used as additives for human in vitro embryo culture media
Authors: Canha-Gouveia Analuce , Prieto-Sánchez María Teresa , Sánchez-Ferrer Maria Luisa , Silva Marta Molla , Soto Juan Carlos Martínez , Paris-Oller Evelyne , Soriano-Úbeda Cristina , Gutierrez Jose Landeras , Fuster Pilar Coy 
Published: August 2020

Abstract
Reproductive fluids have been recently proposed as the most adequate natural additive to complement the chemically defined in vitro culture media in different animal models, since they contain the full range of molecules necessary for the fertilization and development of preimplantation embryos. However, reproductive fluids potentially are problematic in terms of biosecurity and reproducibility of results, and their availability is scarce. Therefore, we have optimized the protocols to characterize the physico-chemical and functional properties of human reproductive fluids to establish the security ranges that could allow their use as natural additives and using the least possible volume of fluids collected. The results of this study offer, for the first time, a complete quality control guideline for human reproductive fluids, including the security levels (referred from physiological values) that should be taken into account for further use – i.e., osmolality, pH, total protein concentrations, presence of endotoxins and sterility conditions. In addition, a functional test has been developed evaluating the in vitro growth of bovine embryos in medium with human reproductive fluids. Finally, as a proof of concept, four embryos cultured with uterine fluid of their own mothers were transferred and two resulted in successful pregnancy and delivery of healthy babies. In conclusion, this study paves the way towards the future use of more natural culture media in human ART.
Conjunctive Use of BTC and Batch Methods for Heavy Metal Transport
Authors: Hussein Mohamed Fahmy , Khater Hassan 
Published: August 2020

Abstract
Marginal sediments can be used to combat punctual pollution by heavy metals in industrial zones. Such practice requires information on metal-concentration in the workshop discharge water. Knowledge about the reaction of the heavy metal with the sediment available in the landscape is of utmost importance. Modeling of batch experiments and breakthrough curves, BTC, supplies relevant information in this regard. We modeled the static batch-data by Freundlich isotherms for testing CdCl2 aqueous solutions equilibria with sandy loam sediment and the dynamic column-data by two codes, CfitM and CfitIM, under saturated water flow conditions. Three Cd-concentrations (5, 20, and 40 ppm) were employed to investigate the conjunction of using two procedures for obtaining the pertinent parameters for the transport of such a heavy metal and the design of the adequate Cd-trap. The results showed the deviations of the two techniques due to differences in their theoretical concepts, mathematical formulation, and performance. The batch method showed utility in supplying first guesses for the retardation factor, R, to insert into the 4-parameter analytical code, CfitIM, applied for column BTC modeling. The iteratively-obtained-parameters of the Freundlich equation were then employed to generate the distribution coefficient, k d. The generated value was, in turn, used to get more fair guess for the retardation factor, R, to use as a fixed-value in the CfitIM code to get an in-depth insight into the BTC dynamics and to obtain the other pertinent model parameters. The BTC runs indicated that the concentration controls the distinctive adsorption and transport rate and behavior of the heavy metal in the sediment column. The most dilute solution offered the highest Cd impediment, as shown by the most significant values for the distribution coefficient, k d, and retardation factor, R. The malfunction of the sediment as a trap appeared at Cd-concentrations four to eight folds higher than the most dilute solution. However, the loamy sand trap is successful when fed with a dilute aqueous solution. A set of successive traps is to arrange in tandem lines when moderate to high concentration is to discharge from an industrial workshop. The results emphasize the utility of the mutual use of these two lab procedures for the design of adequate traps and landfills and the simulation of more complex situations in the field. The point-pollution control needs to continue running batch and BTC experiments and to carry out their corresponding modeling.
Gastrointestinal disturbance and effect of fecal microbiota transplantation in discharged COVID-19 patients
Authors: Deng Zhaoqun , Liu Fengqiong , Ye Shanliang , Zhu Xin , He Xuesong , Wang Shengzhou , Li Yinbao , Lin Jiang , Wang Jingsu , Lin Yonggan , Ren Xin , Li Yong 
Published: August 2020

Abstract
Background: Gastrointestinal manifestations and gut dysbiosis are prevalent after SARS-CoV2 infection.With the continuously increasing number of infected cases, more attention should be paid to this particular population in post-infection recovery.cWe aimed to investigate the potential beneficial effect of FMT on gastrointestinal symptoms, gut dysbiosis and immune status in discharged COVID-19 patients. Results: Gastrointestinal and psychological disorder (45.5%) were observed in COVID-19 patients during post-infection recovery, improvement of which were observed after FMT. Most of the lab results including blood routine and blood biochemistry, within the normal range. The general distribution of 69 different types of lymphocytes differed between before and after FMT. FMT exert significant effect on B cells which was characterized as decreased naive B cell ( P =0.012) and increased memory B cells ( P = 0.001) and non-switched B cells ( P = 0.012).The microbial community richness indicated by OTUs number, observed species and Chao1 estimators was marginally increased after FMT, whereas the community diversity estimated by the Shannon and Simpson index showed no significant changes after FMT. Gut microbiome composition of discharged COVID-19 patients differed from that of the general population at both phylum and genera level, which was characterized with a lower proportion of Firmicutes (41.0%) and Actinobacteria (4.0%), higher proportion of Bacteroidetes (42.9%) and Proteobacteriato (9.2%). FMT can partially restore the gutdysbiosis by increasing the relative abundance of Actinobacteria (15.0%) and reducing Proteobacteriato (2.8%) at the phylum level. At the genera level, Bifidobacterium and Faecalibacterium , which were dominant genera in the human gut microbiota and were beneficial for human health, had significantly increased after FMT. Conclusions: Gastrointestinal and gut dysbiosis were observed in COVID-19 patients during post-infection recovery. FMT can improve the immune functionality, restore the gut microbiota, alleviate gastrointestinal disorders, and may serve as a potential therapeutic and rehabilitative intervention for the COVID-19.
Impact of Nurses' Post-graduate Education on Placement
Authors: Pelusi Gilda , Antonella D'Alleva , Gatti Chiara , Ciriachi Nicoletta , Gasperini Beatrice , Prospero Emilia 
Published: August 2020

Abstract
Background Epidemiological changes led to review health services organization and nursing education, in order to train practitioners capable of effectively dealing with new healthcare needs. Master of Science in Nursing (MSN) degree is a post-graduation training curriculum aimed at providing nurses a method to approach complex and unconventional problems. This survey aims to assess if the skills acquired through MSN degree were implemented in the workplace and were useful for professional advancements. Methods This survey involved 257 MSN graduates of Polytechnic University of Marche, 196 of them completed the survey (response rate 76.3%). Logistic regression models were developed to test independent correlation between variables. Results A positive relation between acquisition of skills and their implementation in the workplace has been demonstrated in all training areas: clinical (OR=25.2; p<0.001), management (OR=7.4; p<0.001), educational (OR=14.2; p<0.001), research (OR=18.8; p<0.001). Only implementation of management skills resulted associated to hierarchical position (nurse managers: OR=11.8; p=0.006; service director nurses: OR=14.6; p=0.025) and age class (≥50 years old: OR=7.3; p=0.004). Economical progressions resulted to be only related to formal hierarchical advancements (OR=27.9; p<0.001), but acquisition of skills allows MSN graduated to increase collaboration in research or educative projects (OR=3.3; p=0.010) and publication of scientific papers (OR=8.7; p<0.001). Conclusions Although application of managerial skills requires the achievement of a higher hierarchical position, implementation of these skills can be realized by all MSN graduates, regardless of their age and hierarchical position. This contribute to improve areas of research and develop new models of nursing care necessary to manage chronic and complex patients.
Chinese SLE Treatment and Research Group (CSTAR) Registry: prevalence and risk factors for chronic scarring alopecia in patients with systemic lupus erythematosus
Authors: Xiang Yirong , Li Mengtao , Luo Hui , Wang Yanhong , Duan Xinwang , Zhao Cheng , Zhan Feng , Wu Zhenbiao , Li Hongbin , Yang Min , Xu Jian , Wei Wei , Wu Lijun , You Hanxiao , Qian Junyan , Yang Xiaoxi , Huang Can , Zhao Jiuliang , Wang Qian , Leng Xiaomei , Tian Xinping , Zhao Yan , Zeng Xiaofeng 
Published: August 2020

Abstract
Background
Scarring alopecia in systemic lupus erythematosus (SLE) patients caused reduced life quality and prolonged disease course. This case control study aims to survey the prevalence of scarring alopecia during the disease course of SLE and evaluate the risk factors for scarring alopecia.
Methods
SLE patients in Chinese SLE treatment and Research group (CSTAR) were recruited. Scarring alopecia was defined according to the SLICC/ACR-DI which was collected during follow-up visit or via self-reported questionnaires. We collected demographic characteristics, common comorbidities, autoantibody profiles, disease activity status, major organ involvements and treatment strategies of these patients at registry. Univariate and multivariate logistic regression analyses were used to investigate the risk factors for scarring alopecia.
Results
We recruited 4792 SLE patients, and 374 (7.80%) patients had scarring alopecia. Mucocutaneous lesions (OR 2.062, p < 0.001), high SLICC/ACR-DI (OR 1.409, p < 0.001) and positive anti-Sm (OR 1.374, p = 0.029) were risk factors for scarring alopecia, while renal (OR 0.714, p = 0.028) and cardio-respiratory involvements (OR 0.347, p = 0.044), and immunosuppressants treatment (OR 0.675, p < 0.001) were significant protective factors.
Conclusions
The prevalence of scarring alopecia in SLE patients is 7.80%. Active treatment strategies should be adopted to prevent scarring alopecia occurring.
Impact of comorbidity burden on mortality in patients with COVID-19: a retrospective analysis of the Korean health insurance database
Authors: Cho Soo Ick , Yoon Susie , Lee Ho-Jin 
Published: August 2020

Abstract
We aimed to investigate the impact of comorbidity burden on mortality in patients with COVID-19. We analyzed the COVID-19 data from the nationwide health insurance claims of South Korea. Data on demographic characteristics, comorbidities, and mortality records of patients with COVID-19 were extracted from the database. The odds ratios of mortality according to comorbidities in patients with COVID-19 with and without adjustment for age and sex were calculated. The predictive value of the original Charlson comorbidity index (CCI) and the age-adjusted CCI (ACCI) for mortality in patients with COVID-19 were investigated using the receiver operating characteristic (ROC) curve analysis. Among 7,590 patients with COVID-19, 227 (3.0%) had died. After age and sex adjustment, hypertension, diabetes mellitus, congestive heart failure, dementia, chronic pulmonary disease, liver, renal, and cancer were significant risk factors for mortality. The ROC curve analysis showed that ACCI threshold ≥4 yielded the best cut-off point for predicting mortality (area under the ROC 0.92; 95% CI, 0.91–0.94). Our study revealed multiple risk factors that were associated with mortality in patients with COVID-19. The high predictive power of the ACCI for mortality in our results could support the importance of old age and comorbidities in the severity of COVID-19.
General public Knowledge of coronavirus disease 2019 (COVID-19) at early stages of the pandemic: results of a random online survey in the Kingdom of Saudi Arabia
Authors: Alqahtani Ali , Krishnaraju V , Alqarni Mona , Hassan Mohammed Al-Sheikh 
Published: August 2020

Abstract
AbstractBackground: A novel coronavirus was identified at the end of 2019 in Wuhan City, China. Later, the World Health Organization (WHO) named the disease caused by the virus coronavirus disease 2019 (COVID-19) and declared a pandemic in March 2020. Saudi and global health agencies have provided various COVID-19 knowledge tools and facts to the general public. Therefore, this study aims to assess COVID-19 knowledge among the general public in Saudi Arabia at the early stages of the pandemic, including knowledge of prevention practices, home quarantine measures, and compliance with governmental restrictions.Subjects and methods: A cross-sectional study was conducted in March 2020 during the COVID-19 pandemic in Saudi Arabia. The study included 1006 participants who responded to a random online COVID-19 public knowledge questionnaire that included five sections: demographic characteristics, general knowledge, prevention practices, home quarantine measures, and knowledge of governmental restrictions. Data were collected from a random sample recruited through the circulation of the questionnaire on social media platforms and were then analysed by descriptive statistical methods. Three levels of knowledge were established: excellent, intermediate, and poor. Differences in the percentages of participants with different knowledge levels by the demographic variables were analysed using the chi-square test.Results: Regarding overall general knowledge of COVID-19, 75%, 24%, and 1% of the participants had excellent, intermediate, and poor knowledge levels, respectively. Knowledge levels were significantly different by nationality and age (P=0.027 and 0.008, respectively). The majority of participants (98.4%) reported excellent knowledge of prevention practices, with no statistically significant differences among groups (P>0.005). Older age groups reported higher knowledge of home quarantine measures (86.6% and 86.4% of the 51-60 and older than 60 age groups, respectively, P=0.001). Approximately 50% of Saudis reported excellent knowledge of the restrictions imposed by the Saudi government to control the spread of COVID-19, while approximately 45% of non-Saudis had an excellent level of knowledge (P= 0.009).Conclusion and recommendations: High levels of knowledge about the virus, including prevention practices, are essential. The provision of COVID-19 facts and knowledge tools should be focused on younger generations to enhance compliance with the governmental restrictions required to stop the spread of COVID-19.
Treinamento por simulação como resposta à demanda dos pacientes críticos COVID19: segurança e qualidade na assistência
Authors: Souza Luís Manuel Mota de , Marques Maria do Céu Mendes Pinto 
Published: August 2020

A Coronavirus Disease (COVID-19), também denominada por severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), foi descrita pela primeira vez no mês de dezembro de 2019, em Wuhan, na China. Esta disseminou-se por todos os continentes, tendo sido declarada pela Organização Mundial da Saúde (OMS), em 11 de março de 2020, como uma pandemia global. No dia 24de junho de 2020, estavam reportados pela OMS 9.129.146 casos e 473.797 mortes causadas pelo SARS-CoV-2/COVID 19. A transmissão do vírus é feita através de gotículas, embora tenha sido encontrado também nas fezes e no sangue, admitindo-se assim, outros meios de potencial transmissão. A sintomatologia associada à infeção por SARS-CoV -2 inclui a presença de febre, mialgia ou fadiga, tosse produtiva e/ou tosse não produtiva dispneia, fadiga ou mialgias na maioria dos casos. Existem ainda relatados de sintomas como a cefaleia, dor de garganta, hemoptises e sintomas gastrointestinais como a diarreia, náusea e dor abdominal, mas com menor incidência.