bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mechanistic Insights Into Molecular Proxies Through Comparison of Subannually Resolved Sedimentary Records With Instrumental Water Column Data in the Santa Barbara Basin, Southern California
Authors: Alfken Susanne , Wörmer Lars , Lipp Julius S. , Wendt Jenny , Schimmelmann Arndt , Hinrichs Kai‐Uwe 
Published: September 2020

AbstractLipid biomarkers archived in marine sediments include widely applied proxies to reconstruct sea surface temperature (SST). Two prominent groups of SST sensitive biomarkers are long‐chain alkenones from haptophyte algae and glycerol dibiphytanyl glycerol tetraethers (GDGTs) from planktonic Thaumarchaeota. The corresponding proxies,
and TEX86, respectively, are strongly correlated with mean annual SST. However, culture experiments suggest that other factors such as nutrients could also influence the proxy signals. We created monthly resolved records of molecular SST proxies via mass spectrometry imaging at 200‐μm resolution in varved sediments from Santa Barbara Basin deposited between 1984 and 2009. Direct comparison to coeval water column data determined at seasonal resolution allows new mechanistic insights into environmental parameters influencing proxy signal formation. The
responds sensitively to SST variations, while also being influenced by seasonal variations in nutrient concentrations. Surface water nitrate concentrations above or below ~2 μmol/L are associated with
values that overestimate or underestimate actual SST, respectively. Distributions of the two major GDGTs, expressed as the Crenarchaeol‐Caldarchaeol‐Tetraether index (CCaT), are highly similar to those of the minor cycloalkylated compounds GDGT‐1 to GDGT‐3, presented as the corresponding ring index. The CCaT is correlated with subsurface chemical properties, which are controlled by interannual variations in upwelling intensity linked to dynamics of the California Current System. This relationship is attributed to the impact of nutrients on thaumarchaeal growth rates and GDGT cyclization. The coupling of CCaT to SST was weak but enhanced for temperatures averaged across the upper 100 m of the water column.
Haplotype-resolved genome analyses of a heterozygous diploid potato
Authors: Zhou Qian , Tang Dié , Huang Wu , Yang Zhongmin , Zhang Yu , Hamilton John P. , Visser Richard G. F. , Bachem Christian W. B. , Robin Buell C. , Zhang Zhonghua , Zhang Chunzhi , Huang Sanwen 
Published: September 2020

AbstractPotato (Solanum tuberosum L.) is the most important tuber crop worldwide. Efforts are underway to transform the crop from a clonally propagated tetraploid into a seed-propagated, inbred-line-based hybrid, but this process requires a better understanding of potato genome. Here, we report the 1.67-Gb haplotype-resolved assembly of a diploid potato, RH89-039-16, using a combination of multiple sequencing strategies, including circular consensus sequencing. Comparison of the two haplotypes revealed ~2.1% intragenomic diversity, including 22,134 predicted deleterious mutations in 10,642 annotated genes. In 20,583 pairs of allelic genes, 16.6% and 30.8% exhibited differential expression and methylation between alleles, respectively. Deleterious mutations and differentially expressed alleles were dispersed throughout both haplotypes, complicating strategies to eradicate deleterious alleles or stack beneficial alleles via meiotic recombination. This study offers a holistic view of the genome organization of a clonally propagated diploid species and provides insights into technological evolution in resolving complex genomes.
Female sex as an independent prognostic factor in the development of oral mucositis during autologous peripheral stem cell transplantation
Authors: Gebri Enikő , Kiss Attila , Tóth Ferenc , Hortobágyi Tibor 
Published: September 2020

AbstractOral mucositis (OM) is a frequent complication of stem cell transplantation-associated toxicity in haematological malignancies, contributing to mortality. Therapy still remains mainly supportive. We assessed risk factors in retrospective analysis of 192 autologous peripheral stem cell transplantation patients with lymphoma and multiple myeloma (MM), respectively. Futhermore, we examined the hormone levels both in serum and saliva during transplantation in 7 postmenopausal female patients with lymphoma compared to healthy controls using electrochemiluminescence immunoassay (ECLIA). Multivariable analysis revealed neutrophil engraftment (p < 0.001; p = 0.021) and female sex (p = 0.023; p = 0.038) as independent predictive factors in the combined patient group and in the lymphoma group, and neutrophil engraftment (p = 0.008) in the MM group. Of the 85 female participants 19 were pre- and 66 postmenopausal. Fifteen of the pre-, and 49 of the postmenopausal women developed ulcerative mucositis (p = 0.769), more often with lymphoma than MM (p = 0.009). Serum estrogen decreased significantly both in postmenopausal controls and transplantated patients compared to premenopausals, with no difference in saliva. Serum progesterone level was significantly (p = 0.026) elevated at day + 7 of transplantation, while salivary progesterone increased at day + 7 and + 14. Our results indicate a predominantly negative effect of female sex hormones on oral immunity with role in the aetiopathogenesis of OM.
The E3 ubiquitin-protein ligase MDM2 is a novel interactor of the von Hippel–Lindau tumor suppressor
Authors: Falconieri Antonella , Minervini Giovanni , Bortolotto Raissa , Piovesan Damiano , Lopreiato Raffaele , Sartori Geppo , Pennuto Maria , Tosatto Silvio C. E. 
Published: September 2020

AbstractMutations of the von Hippel–Lindau (pVHL) tumor suppressor are causative of a familiar predisposition to develop different types of cancer. pVHL is mainly known for its role in regulating hypoxia-inducible factor 1 α (HIF-1α) degradation, thus modulating the hypoxia response. There are different pVHL isoforms, including pVHL30 and pVHL19. However, little is known about isoform-specific functions and protein–protein interactions. Integrating in silico predictions with in vitro and in vivo assays, we describe a novel interaction between pVHL and mouse double minute 2 homolog (MDM2). We found that pVHL30, and not pVHL19, forms a complex with MDM2, and that the N-terminal acidic tail of pVHL30 is required for its association with MDM2. Further, we demonstrate that an intrinsically disordered region upstream of the tetramerization domain of MDM2 is responsible for its isoform-specific association with pVHL30. This region is highly conserved in higher mammals, including primates, similarly to what has been already shown for the N-terminal tail of pVHL30. Finally, we show that overexpression of pVHL30 and MDM2 together reduces cell metabolic activity and necrosis, suggesting a synergistic effect of these E3 ubiquitin ligases. Collectively, our data show an isoform-specific interaction of pVHL with MDM2, suggesting an interplay between these two E3 ubiquitin ligases.
Nasogastric tube, a warning sign for high-flow nasal cannula failure in infants with bronchiolitis
Authors: Nascimento Milena Siciliano , Quinto Danielle E. R. , Oliveira Gisele C. Z. , Rebello Celso M. , do Prado Cristiane 
Published: September 2020

AbstractHigh-flow nasal cannula (HFNC) therapy is routinely used in the treatment of infants with bronchiolitis. This study sought to identify markers associated with failure of HFNC therapy that serve as warnings for early staging of other ventilatory support products. A retrospective study of infants with a diagnosis of bronchiolitis, receiving HFNC and admitted to the pediatric intensive care unit from January 2016 to June 2017, was conducted. The subjects were divided into two study groups according to the success or failure of HFNC therapy. Risk factors were assessed using the following variables: age, time between hospital admission and start of HFNC, equipment model, and the need for a nasogastric tube. Eighty-one infants were studied, and 18 (21.7%) of them exhibited therapy failure. The results of the logistic models showed that the chances of failure for patients requiring a nasogastric tube during HFNC use were more likely than those for patients with oral nutrition (OR = 8.17; 95% CI 2.30–28.99; p = 0.001). The HFNC failure was not associated with the device used (OR = 1.56; 95% CI 0.54–4.52; p = 0.41), time between hospital admission and HFNC installation (OR = 1.01; 95% CI 0.98–1.03; p = 0.73), or age (OR = 0.98; 95% CI 0.82–1.17; p = 0.82). Among late outcomes evaluated, the patients with therapy failure had longer total durations of O2 use (p < 0.001) and longer hospital stays (p < 0.001). The need to use a nasogastric tube during HFNC use was associated with HFNC therapy failure and can be considered as a marker of severity in children with bronchiolitis.
Structural polymorphisms in fibrillar aggregates associated with exfoliation syndrome
Authors: Ghaffari Sharaf Mehdi , Amidian Sara , Rathod Vineet , Crichton Andrew , Damji Karim F. , Wille Holger , Unsworth Larry D. 
Published: September 2020

AbstractExfoliation syndrome is largely considered an age-related disease that presents with fibrillar aggregates in the front part of the eye. A growing body of literature has investigated structural diversity of amyloids and fibrillar aggregates associated with neurodegenerative disease. However, in case of exfoliation syndrome, there is a dearth of information on the biophysical characteristics of these fibrils and structural polymorphisms. Herein, structural diversity of fibrils isolated from the anterior lens capsule of patients was evaluated using transmission electron microscopy techniques. It was apparent that, despite having a low sample number of different patients, there exists a wide range of fibril morphologies. As it is not precisely understood how these fibrils form, or what they are composed of, it is difficult to postulate a mechanism responsible for these differences in fibril structure. However, it is apparent that there is a wider range of fibril structure than initially appreciated. Moreover, these data may suggest the variance in fibril structure arises from patient-specific fibril composition and/or formation mechanisms.
On modifications to the Poisson-triggered hidden Markov paradigm through partitioned empirical recurrence rates ratios and its applications to natural hazards monitoring
Authors: Bhaduri Moinak 
Published: September 2020

AbstractHidden Markov models (HMMs), especially those with a Poisson density governing the latent state-dependent emission probabilities, have enjoyed substantial and undeniable success in modeling natural hazards. Classifications among these hazards, induced through quantifiable properties such as varying intensities or geographic proximities, often exist, enabling the creation of an empirical recurrence rates ratio (ERRR), a smoothing statistic that is gradually gaining currency in modeling literature due to its demonstrated ability in unearthing interactions. Embracing these tools, this study puts forth a refreshing monitoring alternative where the unobserved state transition probability matrix in the likelihood of the Poisson based HMM is replaced by the observed transition probabilities of a discretized ERRR. Analyzing examples from Hawaiian volcanic and West Atlantic hurricane interactions, this work illustrates how the discretized ERRR may be interpreted as an observed version of the unobserved hidden Markov chain that generates one of the two interacting processes. Surveying different facets of traditional inference such as global state decoding, hidden state predictions, one-out conditional distributions, and implementing related computational algorithms, we find that the latest proposal estimates the chances of observing a high-risk period, one threatening several hazards, more accurately than its established counterpart. Strongly intuitive and devoid of forbidding technicalities, the new prescription launches a vision of surer forecasts and stands versatile enough to be applicable to other types of hazard monitoring (such as landslides, earthquakes, floods), especially those with meager occurrence probabilities.
Effect of very low-protein diets supplemented with branched-chain amino acids on energy balance, plasma metabolomics and fecal microbiome of pigs
Authors: Spring Shelby , Premathilake Hasitha , Bradway Chloe , Shili Cedrick , DeSilva Udaya , Carter Scott , Pezeshki Adel 
Published: September 2020

AbstractFeeding pigs with very-low protein (VLP) diets while supplemented with limiting amino acids (AA) results in decreased growth. The objective of this study was to determine if supplementing VLP diets with branched-chain AA (BCAA) would reverse the negative effects of these diets on growth and whether this is associated with alterations in energy balance, blood metabolomics and fecal microbiota composition. Twenty-four nursery pigs were weight-matched, individually housed and allotted into following treatments (n = 8/group): control (CON), low protein (LP) and LP supplemented with BCAA (LP + BCAA) for 4 weeks. Relative to CON, pigs fed with LP had lower feed intake (FI) and body weight (BW) throughout the study, but those fed with LP + BCAA improved overall FI computed for 4 weeks, tended to increase the overall average daily gain, delayed the FI and BW depression for ~ 2 weeks and had transiently higher energy expenditure. Feeding pigs with LP + BCAA impacted the phenylalanine and protein metabolism and fatty acids synthesis pathways. Compared to CON, the LP + BCAA group had higher abundance of Paludibacteraceae and Synergistaceae and reduced populations of Streptococcaceae, Oxyphotobacteria_unclassified, Pseudomonadaceae and Shewanellaceae in their feces. Thus, supplementing VLP diets with BCAA temporarily annuls the adverse effects of these diets on growth, which is linked with alterations in energy balance and metabolic and gut microbiome profile.
Complete intraureteral stent placement relieves daytime urinary frequency compared with conventional placement in patients with an indwelling ureteral stent: post-hoc analysis of a randomized, controlled trial
Authors: Matsuzaki Tomoaki , Yoshida Takashi , Murota Takashi , Nakao Kazuyoshi , Taguchi Makoto , Kinoshita Hidefumi , Matsuda Tadashi 
Published: September 2020

AbstractA previous randomized, controlled trial had demonstrated that complete intraureteral stent placement (CIU-SP) was superior to conventional stent placement (C-SP) in terms of improvement of stent-related urinary symptoms. However, it is unclear as to which subdomain symptom and cohort could benefit the most from CIU-SP compared to C-SP in urinary symptoms while considering the baseline urinary status. To determine this, a post-hoc analysis was performed using data from a previous study (CIU-SP group, n = 39; C-SP group, n = 41). We assessed the mean changes in the International Prostate Symptom Score (I-PSS) and the Overactive Bladder Symptom Score (OABSS) from baseline to day 14. Statistical comparison between the two groups was performed using analysis of covariance with adjustment of baseline urinary status as a covariate. Among 80 patients, the total I-PSS was significantly lower in the CIU-SP group than in the C-SP group in the cohort with mild urinary symptoms (P = 0.005), but not in those with moderate/severe symptoms (P = 0.521). The CIU-SP group showed significantly improved I-PSS and OABSS daytime frequencies, with the highest t statistic (2.47 and 2.10, respectively) among subdomains of both symptom scores compared with the C-SP group (both P < 0.001). In multivariate regression analysis, the stent placement method (CIU-SP vs. C-SP) was independently associated with the I-PSS daytime frequency on day 14 (P = 0.017). This study suggests that CIU-SP significantly improved stent-related daytime frequency compared with C-SP, and it may benefit especially those patients who have mild urinary symptoms before the placement of ureteral stents.
Annurca apple polyphenol extract promotes mesenchymal-to-epithelial transition and inhibits migration in triple-negative breast cancer cells through ROS/JNK signaling
Authors: Vuoso Daniela Cristina , D’Angelo Stefania , Ferraro Rosalia , Caserta Sergio , Guido Stefano , Cammarota Marcella , Porcelli Marina , Cacciapuoti Giovanna 
Published: September 2020

AbstractAberrant activation of epithelial-to-mesenchymal transition has been shown to correlate with triple-negative breast cancer (TNBC) progression and metastasis. Thus, the induction of the reverse process might offer promising opportunities to restrain TNBC metastatic spreading and related mortality. Recently, the Annurca apple polyphenol extract (APE) has been highlighted as a multi-faceted agent that selectively kills TNBC cells by ROS generation and sustained JNK activation. Here, by qualitatively and quantitatively monitoring the real-time movements of live cells we provided the first evidence that APE inhibited the migration of MDA-MB-231 and MDA-MB-468 TNBC cells and downregulated metalloproteinase-2 and metalloproteinase-9. In MDA-MB-231 cells APE decreased SMAD-2/3 and p-SMAD-2/3 levels, increased E-cadherin/N-cadherin protein ratio, induced the switch from N-cadherin to E-cadherin expression and greatly reduced vimentin levels. Confocal and scanning electron microscopy imaging of APE-treated MDA-MB-231 cells evidenced a significant cytoskeletal vimentin and filamentous actin reorganization and revealed considerable changes in cell morphology highlighting an evident transition from the mesenchymal to epithelial phenotype with decreased migratory features. Notably, all these events were reverted by N-acetyl-l-cysteine and JNK inhibitor SP600125 furnishing evidence that APE exerted its effects through the activation of ROS/JNK signaling. The overall data highlighted APE as a potential preventing agent for TNBC metastasis.
TRPV2 interacts with actin and reorganizes submembranous actin cytoskeleton
Authors: Yadav Manoj , Goswami Chandan 
Published: September 2020

Abstract
The understanding of molecules and their role in neurite initiation and/or extension is not only helpful to prevent different neurodegenerative diseases but also can be important in neuronal damage repair. In this work, we explored the role of transient receptor potential vanilloid 2 (TRPV2), a non-selective cation channel in the context of neurite functions. We confirm that functional TRPV2 is endogenously present in F11 cell line, a model system mimicking peripheral neuron. In F11 cells, TRPV2 localizes in specific subcellular regions enriched with filamentous actin, such as in growth cone, filopodia, lamellipodia and in neurites. TRPV2 regulates actin cytoskeleton and also interacts with soluble actin. Ectopic expression of TRPV2-GFP in F11 cell induces more primary and secondary neurites, confirming its role in neurite initiation, extension and branching events. TRPV2-mediated neuritogenesis is dependent on wildtype TRPV2 as cells expressing TRPV2 mutants reveal no neuritogenesis. These findings are relevant to understand the sprouting of new neurites, neuroregeneration and neuronal plasticity at the cellular, subcellular and molecular levels. Such understanding may have further implications in neurodegeneration and peripheral neuropathy.
A novel analytical model of the magnetic field configuration in the Galactic center
Authors: Guenduez M. , Becker Tjus J. , Ferrière K. , Dettmar R.-J. 
Published: September 2020

Context. Cosmic-ray propagation is strongly dependent on the large-scale configuration of the Galactic magnetic field. In particular, the Galactic center region provides highly interesting cosmic-ray data from gamma-ray maps and it is clear that a large fraction of the cosmic rays detected at Earth originate in this region of the Galaxy. Yet because of confusion from line-of-sight integration, the magnetic field structure in the Galactic center is not well known and no large-scale magnetic field model exists at present.
Aims. In this paper, we develop a magnetic field model, derived from observational data on the diffuse gas, nonthermal radio filaments, and molecular clouds.
Methods. We derive an analytical description of the magnetic field structure in the central molecular zone by combining observational data with the theoretical modeling of the basic properties of magnetic fields.
Results. We provide a first description of the large-scale magnetic field in the Galactic center region. We present first test simulations of cosmic-ray propagation and the impact of the magnetic field structure on the cosmic-ray distribution in the three dimensions.
Conclusions. Our magnetic field model is able to describe the main features of polarization maps; it is particularly important to note that they are significantly better than standard global Galactic magnetic field models. It can also be used to model cosmic-ray propagation in the Galactic center region more accurately.
Present Status of Neutron-, Photo-induced and Spontaneous Fission Yields Experimental Data
Authors: Pritychenko B. , Schwerer O. , Totans J. , Gritzay O. 
Published: September 2020

Nuclear reaction data collection, evaluation and dissemination have been pioneered at the Brookhaven National Laboratory since the early 50s. These activities gained popularity worldwide, and around 1970 the experimental nuclear reaction data interchange or exchange format (EXFOR) was established. The originalEXFORcompilation scope consisted only of neutron reactions and spontaneous fission data, while many other nuclear data sets were ignored. Due to the high cost of new experiments, it is very important to find and recover the previously disregarded data using scientific publications, data evaluations and nuclear databases comparisons.Fission yields play a very important role in applied and fundamental physics, and such data are essential in many applications. The comparative analysis of Nuclear Science References (NSR) and Experimental Nuclear Reaction (EXFOR) databases shows a large number of unaccounted experiments and provides a guide for the recovery of fission cross sections, yields and covariance data sets. The dedicated fission yields data compilation effort is currently underway in the Nuclear Reaction Data Centers (NRDC) network, and includes identification, compilation, storage and Web dissemination of the recovered data sets.
Bewegungsverhalten, Kosten mangelnder körperlicher
Aktivität und Bewegungsförderung in
Österreich
Authors: Mayer Susanne , Felder-Puig Rosemarie , Gollner Erwin , Dorner Thomas Ernst 
Published: September 2020

ZusammenfassungDem Dreischritt „Status quo – Konsequenzen –
Maßnahmen“ folgend werden Daten zum Ausmaß
körperlicher Aktivität und deren Einflussfaktoren,
mögliche gesellschaftliche Kosten mangelnder körperlicher
Aktivität sowie Einflussebenen und Maßnahmen zur
Bewegungsförderung in Österreich präsentiert. In
Österreich ist der Anteil an Personen, die die Bewegungsempfehlungen
nicht erfüllen, hoch und es besteht diesbezüglich klar
Verbesserungspotenzial, insbesondere in Bezug auf muskelkräftigende
Aktivitäten bei Erwachsenen. Je nach Datenquelle erfüllen
von den Erwachsenen 42–50% die Empfehlungen für
ausdauerorientierte Bewegung und 18–33% die für
muskelkräftigende Aktivitäten. Gleichzeitig ist die
österreichische Bevölkerung im internationalen Vergleich
aktiver als jene vieler anderer Länder. Große Unterschiede
bestehen im Bewegungsverhalten hinsichtlich demografischer,
sozioökonomischer und geografischer Determinanten. Die
gesellschaftlichen Folgen durch mangelnde körperliche
Aktivität sind beträchtlich, sowohl hinsichtlich verlorener
Lebensjahre durch vorzeitigen Tod, als auch den Kosten, die der
Allgemeinheit entstehen, beispielsweise an Gesundheitskosten und
Produktivitätsverlusten. Die für das Jahr 2017
hochgerechnete Summe von 248 Mio. Euro stellt aufgrund methodischer
Schwierigkeiten wahrscheinlich eine Unterschätzung dar.
Wissenschaftlich abgesicherte Einflussebenen auf das Bewegungsverhalten
umfassen im Sinne der lebens(um)weltortientierten Herangehensweise die
Lebenswelten Kindergarten und Schule, Betriebe,
Gemeinde/Städte, Pflegewohnheime und die Natur.