bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cutting Edge: Inhibition of Glycogen Synthase Kinase 3 Activity Induces the Generation and Enhanced Suppressive Function of Human IL-10+ FOXP3+–Induced Regulatory T Cells
Authors: Cheng Hao , Wang Lingbiao , Yang Biaolong , Li Dan , Wang Xiaoxia , Liu Xinnan , Tian Na , Huang Qianru , Feng Ru , Wang Zhengting , Liang Rui , Dai Sheng-Ming , Lv Ling , Wu Ji , Zang Yuan-Sheng , Li Bin 
Published: August 2020

Abstract
IL-10 is critical for Foxp3+ regulatory T cell (Tregs)–mediated immune suppression, but how to efficiently upregulate IL-10 production in Tregs remains unclear. In this article, we show that human IL-10+ FOXP3+–induced regulatory T cell (iTreg) generation can be dramatically promoted by inhibiting GSK3 activity. IL-10+ FOXP3+ iTregs induced by GSK3 inhibition exhibit classical features of immune-suppressive T cells. We further demonstrate that IL-10+ iTregs exhibit enhanced suppressive function in both IL-10–dependent and –independent manners. The enhanced suppressive function of IL-10+ Tregs is not due to a single factor such as IL-10, although IL-10 may mediate this enhanced suppressive function to some extent. Mechanistically, the increased transcriptional activity of IL-10 promoter and the enhanced expression of C-Maf and BLIMP1 coordinately facilitate IL-10 expression in human iTregs under GSK3 inhibition. Our study provides a new strategy to generate human immune-suppressive IL-10+ FOXP3+ Tregs for immunotherapies.
Tetramer Immunization and Selection Followed by CELLISA Screening to Generate Monoclonal Antibodies against the Mouse Cytomegalovirus m12 Immunoevasin
Authors: Aguilar Oscar A. , Tanaka Miho , Balaji Gautham R. , Berry Richard , Rossjohn Jamie , Lanier Lewis L. , Carlyle James R. 
Published: August 2020

Abstract
The generation of reliable mAb of unique and desired specificities serves as a valuable technology to study protein expression and function. However, standard approaches to mAb generation usually involve large-scale protein purification and intensive screening. In this study, we describe an optimized high-throughput proof-of-principle method for the expanded generation, enrichment, and screening of mouse hybridomas secreting mAb specific for a protein of interest. Briefly, we demonstrate that small amounts of a biotinylated protein of interest can be used to generate tetramers for use as prime-boost immunogens, followed by selective enrichment of Ag-specific B cells by magnetic sorting using the same tetramers prior to hybridoma generation. This serves two purposes: 1) to effectively expand both low- and high-affinity B cells specific for the antigenic bait during immunization and 2) to minimize subsequent laborious hybridoma efforts by positive selection of Ag-specific, Ab-secreting cells prior to hybridoma fusion and validation screening. Finally, we employ a rapid and inexpensive screening technology, CELLISA, a high-throughput validation method that uses a chimeric Ag fused to the CD3ζ signaling domain expressed on enzyme-generating reporter cells; these reporters can detect specific mAb in hybridoma supernatants via plate-bound Ab-capture arrays, thereby easing screening. Using this strategy, we generated and characterized novel mouse mAb specific for a viral immunoevasin, the mouse CMV m12 protein, and suggest that these mAb may protect mice from CMV infection via passive immunity.
The role of volumetric method in the assessment of chemotherapy response and predicting survival in malignant pleural mesothelioma
Authors: Urfali Furkan Erturk , Metintas Selma , Gurgen Atila , Guntulu AK , Ozkan Ragip , Metintas Muzaffer 
Published: August 2020

Background:
Malignant pleural mesothelioma (MPM) is a pleural tumor with high mortality rate and short-term survival expectancy after diagnosis. Assessment of the response to chemotherapy, which is the first choice in treatment of MPM, is important for the transition to alternative chemotherapy protocols and immunotherapy. There is no clarity in the response to chemotherapy treatment.

Objective:
Our study aims to compare the assessment of chemotherapy response using the Modified Response Evaluation Criteria in Solid Tumors (mRECIST) criteria and volumetric measurements and to correlate with median survival.

Materials and Methods:
Thirty-two patients (16 females and 16 males) were included in the study, and their ages ranged from 28 to 78 years. Chemotherapy response was determined by both mRECIST and volumetric approach. Tumor volume was measured by linear interpolation and semi-automatic segmentation. Log-rank multiple cutoff analysis was used to determine appropriate cutoff values of volumetric response criteria.

Results:
According to both mRECIST and volumetric approach, median survival times in partial response, stable disease, and progressive disease groups were 24, 15, and 9 months, respectively. The survival times of the three groups were different (logrank: 17.76; P < 0.001) by mRECIST. The survival of the progressive disease group was shorter than that of the other groups (logrank: 18.91; P < 0.001) by volumetric approach.

Conclusions:
In the assessment of chemotherapy response, even though classifications obtained according to the mRECIST criteria and volumetric measurements are statistically compatible, we think that the measurement of the volumetric values will increase the standardization. In our study, threshold values for volumetric measurements were determined; however, these values should be supported by large-scale multicenter studies.
Value of Speckle Tracking Echocardiography as a Predictor of the Deleterious Effect of Right Ventricular Pacing on Left Ventricular Function
Authors: Rafla Samir , Zaki Amr , Aboelhoda Aly , Sappaq Moustafa , Magdy Gehan 
Published: August 2020

Background:
Pacemaker-induced cardiomyopathy (PICM) is reported in different articles but with variable incidence.

Aim:
The aim of the study is to determine the validity of speckle tracking echocardiography as a predictor of the deleterious effect of right ventricular (RV) pacing on left ventricular (LV) function, using two-dimensional guided global and segmental longitudinal strain.

Materials and Methods:
Fifty patients with conventional indications for permanent pacemaker implantation were studied; they were classified to Group A (37 = 74%) patients who didn't show a significant reduction of ejection fraction (EF) (>10%) and Group B (13 = 26%) represent patients who showed a significant reduction of EF. Group C constituted 25 healthy persons (control group). We defined PICM when EF is reduced >10%.

Results:
The incidence of pacemaker-induced ventricular dysfunction was 26%. Statistical analysis revealed that left atrial volume index (LAVI), global longitudinal strain (GLS), native-QRS duration, and ischemic heart disease were significant predictors of reduction of LV function, however, on multivariate regression analysis, only the LAVI and GLS were independent predictors of reduction of LVEF after permanent pacing.

Conclusions:
Speckle tracking echocardiography is a new, unique and evolving tool to assess the myocardial deformation which can detect LV systolic dysfunction much earlier than can be reflected in LVEF. The importance of defining predictors is to predict whom patient will be at risk for the deleterious effect of RV pacing on LV function, and who will need observation with possible upgrading to biventricular pacing.
Urban- Rural Marketing Linkage Practices and Challenges Evidences from Ambo Town West Shoa Zone, Orimia Regional State, Ethiopia
Authors: Geleta Negash 
Published: August 2020

The objective of study was to assess the practices and challenges of rural-urban marketing linkages in Ambo Town. The study may be used to show the gaps between urban and rural on the marketing of agricultural products. The study was used descriptive research design. The populations of the study were some selected retailers and farmers those participate on the marketing of agricultural products. For sample size determination Yemane Taro formula was used and accordingly 187 sample size was employed. To identify this sample random sampling was used to select the research subjects from the population. As the source of information primary data was employed using structured questionnaire. For data analysis descriptive statistics was employed. Based on the data collected the findings of the study show that the production linkages were very weak which was reflected mainly in case of poor connection in the between urban and rural. However, a strong consumption linkage was observed as farmers tend to purchase goods and services from the Town. The insufficient crop production had made the existing marketing linkage to be expressed only in the form of exchange of products. Financial linkage was further strengthened even through all are not connected with rural farmers. Finally, the research recommended that urban and rural oriented linkage must be practiced which need further strengthen for the existing financial linkages and production linkage as it provides opportunity for existing traders in the Town.
Ecoutopia from Fiction to Fact: An Interview with Heather Alberro
Authors: Alberro Heather 
Published: August 2020

Human is at the heart of the story of climate change in the Anthropocene where, according to Dipesh Chakrabarty (2012), human behaviors have influenced the environment and created a distinct geological epoch. Current climate change issues are largely human induced. This implies that the human species is now part of the natural history of the planet. In November 2016, Stephen Hawking warned that humanity has 1000 years to leave the earth due to climate change, but in his most recent BBC documentary aired on June 15, 2017 called Expedition New Earth, he suggested humans have just 100 years left before doomsday. In spite of such warnings and writings, Donald Trump withdrew America from the Paris Climate Agreement on June 2017, on the same day, satellite images showed that a huge mass of ice in an area of ​​five thousand square kilometres was breaking away from the Antarctic continent under the impact of rising temperature. It seems that Trump’s act is beyond ecological consideration as he believes the agreement could “cost America as much as 2.7 million lost jobs by 2025”. Projections of climate change, however, have shown horrible scenarios involving a central economic metropolis such as New York losing much of its lands because of rising sea levels. The inhabitants of such areas will have to uproot their communities and cultures to move to less vulnerable lands. Thus, it is important to examine how ecoutopian literature is responding to the conditions of the human being in this epoch. In the following interview, Heather Alberro has answered to some questions on climate change, the conditions of human being in the Anthropocene, and the role of literature and culture in relation to environmental issues.
Pope, Johnson and Swift: A Comparison of their Poetic Styles and Views on Human Nature
Authors: Feng Liqun 
Published: August 2020

This paper aims to compare the poetic styles and views on human nature of three literary giants in English literature, namely, Alexander Pope, Jonathan Swift and Samuel Johnson. As the three men of letters almost lived in the same age and they were all fond of writing on human nature, it will be very interesting to compare their respective styles and views on this issue. Since no previous studies have been found on this topic, this paper will be of great significance in exploring how their individual style and thinking vary from one another. Through close textual analysis of their representative poetic works, including An Essay on Man by Pope, The Vanity of Human Wishes by Johnson, and finally Verses on the Death of Dr. Swift, D.S.P.D. by Swift, this paper discovers the extent to which the three great authors differ from one another in their poetic styles and views on human nature. From Alexander Pope to Samuel Johnson and then down to Jonathan Swift, their respective poetic styles drop in formality and start to be increasingly less serious. Their views on human nature, accordingly, become increasingly hopeless and bleak. For Alexander Pope, self-love and reason are the central traits in human nature; to live a righteous life man has to use reason to counterbalance his self-love. For Samuel John, it is vanity that motivates all human actions; to resolve all the unhappiness in human life, however, man must use reason. Therefore, both poets emphasize reason as a combating force against all the ego-centrism inherent in human nature. As for Jonathan Swift, he even did not believe human beings are capable of reason; what he perceives in human nature is mere selfishness. This means that Pope and John are still serious about human nature and believe all the evilness in human nature can be mended or bettered, while Jonathan Swift starts to jeer at it, which signifies his complete loss of faith in human nature. Although they all believe ego-centrism to be intrinsic in human nature, we can conclude that Jonathan Swift, among the three, possesses the bleakest view of human nature.
Hubungan Ketergantungan Gawai Dengan Stres Akademik Pada Mahasiswa Keperawatan
Authors: Hargiana Giur , Sintiawati Tia 
Published: August 2020

Pada perkembangan era digital saat ini istilah kecanduan sudah memiliki perkembangan yang sangat dinamis, bukan hanya kecanduan narkoba namun kecanduan gawai merupakan bentuk kecanduan yang berbahaya dan memiliki efek yang luas dimasyarakat. Generasi Z, merupakan populasi usia dewasa muda saat ini, generasi ini berkembang di era digital, sehingga pembentukan kopingnya pun terpengaruh oleh hal tersebut. Pada usia dewasa muda, biasanya orang sedang dalam fase pendidikan tinggi sebagai mahasiswa, mahasiswa disibukkan oleh berbagai kesibukan baik akademik maupun non akademik. Hal ini membuat mahasiswa rentan mengalami stres. Salah satu stresor yang mahasiswa rasakan selama perkuliahannya yaitu berkaitan dengan stres akademik. Penelitian ini bertujuan untuk mengidentifikasi gambaran tingkat ketergantungan gawai dan tingkat stres akademik pada mahasiswa di FIK UI dan untuk mencari hubungan keduanya menggunakan kuesioner Academic Stress Scale (ASS) dan Test for Gadget Dependency. Desain penelitian ini adalah cross sectional, teknik sampel menggunakan proportional stratified random sampling dengan melibatkan 229 mahasiswa. Hasil analisis uji statistik chi-square didapatkan p= 0,000 dan nilai p < a = 0,05 yang berarti terdapat hubungan yang signifikan antara ketergantungan terhadap gawai dengan stres akademik pada mahasiswa. Hasil penelitian ini juga menemukan mahasiswa mengalami stres akademik sedang sebanyak 66,4% dan mahasiswa mengalami ketergantungan terhadap gawai pada kategori sedang sebanyak 69,9%. Ketergantungan terhadap gawai dan stress akademik memiliki hubungan yang saling memengaruhi, seseorang dengan ketergantungan gawai yang tinggi akan memiliki stress akademik yang tinggi pula dan sebaliknya. Rekomendasi dari penelitian ini mahasiswa perlu aware dengan perilaku dalam menggunakan gawai.Kata kunci: ketergantungan gawai; mahasiswa keperawatan; stres akademik
Knowledge of, risk perception towards and reported preventive practices against Lassa fever among health workers in Ondo State, Nigeria
Authors: Fatiregun Akinola A , Isere Elvis E , Agunbiade Opeyemi , Dosumu Modupeola , Onyibe Rosemary 
Published: August 2020

Background: Secondary transmission of Lassa fever has been recorded annually among healthcare workers who provided care. An outbreak of the disease in Ondo State, Southwest Nigeria in 2018, recorded high morbidity and mortality. This study was conducted to assess the knowledge, risk perception, and preventive practices towards Lassa fever among healthcare workers.
Methods: A health facility-based descriptive cross-sectional study design was conducted involving senior members of staff randomly selected from the list of staff members. A structured pre-tested questionnaire was administered to 554 consenting respondents in 304 health facilities.
Results: More than half of the respondents (317, 57.2%) are health workers in primary healthcare cadres. Although 444 (80.1%) knew the case definition for reporting, only 379 (68.4%) correctly indicated the reporting channel. Concerning risk perception, 174 (31.4%) rated their risk of contracting the disease in the workplace as high, and 309 (55.8%) indicated that hospitals' infection control policy is inadequate. Furthermore, only 76 (13.7%) of the respondents reported having modified their working habits for fear of being infected with Lassa fever three months before the study with 368 (66.4%) and 474 (85.6%) reported that they always use aprons and gloves respectively during treatment of patients.
Conclusion: There is a need to strengthen the hospital infection control policy and train healthcare workers on reporting suspected cases.
Valuation de uma spin-off brasileira de cloud computing voltada para o mercado de pequenas e médias empresas
Authors: Carrete Liliam Sanchez , Krakauer Decio , Marques Jane Aparecida 
Published: August 2020

Objetivo do Trabalho: O presente estudo tem como objetivo estudar a estratégia de realização de valuation de empresas de tecnologia, em especial de uma spin-off brasileira de cloud computing voltada para o mercado de pequenas e médias empresas, de forma a orientar os empreendedores desse setor para as fases de negociação de participação para captação de recursos para investidor Venture Capital com a finalidade de expansão ou saída do negócio.Metodologia: Foi realizado um estudo de caso em uma spin-off específica, com foco em soluções de software de gestão empresarial para pequenas e médias empresas.Originalidade: O mercado de cloud computing cresceu, no Brasil, 47% em 2016, o que indica um grande potencial para novas empresas focadas nesse segmento. Dessa forma, passa a ser relevante o estudo de estratégias de crescimento, de expansão ou de vendas dessas empresas.Principais Resultados: A aplicação dos resultados obtidos com o estudo pode ser útil para nortear estratégias de captação de recursos com fins de expansão dessas empresas de cloud computing através da sua avaliação tanto para futuros empreendedores quanto para investidores.Contribuições Teóricas: A aplicação de um método de valuation de uma spin-off de uma empresa que atua no segmento de cloud computing para captação de recursos de investidores Venture Capital representa uma contribuição tanto para empreendedores quanto para investidores.
Proposição de um instrumento de mensuração do desenvolvimento de capacidades dinâmicas
Authors: Zaluski Felipe Cavalheiro , Sausen Jorge Oneide , Ferreira Gloria Charão 
Published: August 2020

Objetivo: este estudo objetiva construir e validar uma escala de mensuração dos elementos componentes e dos mecanismos organizacionais de desenvolvimento de capacidades dinâmicas (CD) em organizações.Método: a metodologia da pesquisa baseou-se na abordagem quantitativa e descritiva, com coleta de dados bibliográfica e por meio de e-survey em 137 organizações do Sul do Brasil. A validação da escala foi realizada pela modelagem de equações estruturais (MEE) com o software SmartPLS 3.2.8.Originalidade/Relevância: ainda que existam esforços no desenvolvimento de escalas sobre CD, há uma lacuna teórica quanto à criação de escalas e instrumentos de medição, uma vez que tais capacidades estão ligadas aos processos internos das organizações. Diante disso, este estudo se mostra relevante ao se direcionar à proposição e validação de uma escala baseada no desenvolvimento das CD que possibilite novas investigações empíricas e discussões teóricas.Resultados: a escala final proposta para mensuração dos elementos componentes e dos mecanismos organizacionais de desenvolvimento de CD apresentou três dimensões e vinte indicadores. Os achados demonstram que a articulação dos indicadores dos elementos componentes e dos mecanismos de desenvolvimento das capacidades dinâmicas resulta em uma escala que pondera vários aspectos relacionados à existência do desenvolvimento de CD em organizações.Contribuições teóricas/metodológicas: esta pesquisa proporciona um recurso acadêmico para a utilização da escala proposta em futuras pesquisas e amplia a possibilidade de desenvolvimento da temática das capacidades dinâmicas nos estudos organizacionais.
The poetics the Iraqi War: Between Discursive Conflicts and Diasporic Discourse
Authors: Haidar Otared 
Published: August 2020

This study attempts to explore the contemporary poetic scene in Iraq during the last two decades that witnessed two prolonged wars, and to investigate its prospects and challenges. What prompted and motivated this study is the discursive tension going on across the poetic community in which the only commonplace seems to be the continuous complaint of both the poets and the critics about the shortage of support and of marginalization. The internal conflict across the poetic community focuses on two issues: the authentic portrayal of the situation in Iraq, and the poetic excellence. Drawing on many approaches taken from socio-cultural studies, textual studies and comparative poetics, the paper follows an interdisciplinary theoretical approach to defining and constructing the contemporary Iraqi cultural scene as a field meriting study by contextualizing a set of representative poetic texts. By studying and analyzing the literary and extra-literary relations of the text, this study aspires to define and classify the main trends and tendencies of the contemporary Iraqi poetry. Reading these texts within their sociocultural and institutional contexts will cast a light on the contemporary Iraqi poetic reading and writing culture which involves not only readers and writers, but also censors, publishing houses, official and unofficial authorities, newspapers, scholars, cultural administrations, and a host of other individuals and institutions besides. The critical reading of the poems aspires to manifest that in view of the general atmosphere of confusion and frustration during this period that witnessed the collapse of the cultural institution among other institutions, the contemporary Iraqi poetic discourse in Iraq hold some keys to the reading of the socio-cultural wartime experience, and more importantly it holds insights into what will follow in the post-war period. Keywords: Iraq War, Iraqi and international war poetry, Iraqi poets and literary critics, Iraqi media, discursive conflicts, home and diaspora, exilic literature, women writing.
The Relationship Between a Seventh-Grade Pupil's Academic Self-Concept and Achievement in Mathematics
Authors: Bachman Alfred Morry 
Published: August 2020

The relationship between mathematics self-concept and mathematics achievement was investigated with a sample of 404 seventh-grade students in the Portland, Oregon, Public Schools. Correlations were obtained using the Pearson product-moment coefficient, for several measures of self-concept of ability and mathematics achievement.A significant relationship was found between self-concept of ability in mathematics and mathematics achievement. For the specific self-concept measures used, mathematics self-concept was found to be the best predictor of mathematics achievement.The results suggest needed research on the question whether there is a cause-and-effect relationship between a student's self-concept of his ability to do mathematics and his achievement. In particular, will a change in self-concept (higher) result in a corresponding change in achievement?