bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mechanisms of migraine as a chronic evolutive condition
Authors: Andreou Anna P. , Edvinsson Lars 
Published: December 2019

AbstractUnderstanding the mechanisms of migraine remains challenging as migraine is not a static disorder, and even in its episodic form migraine remains an “evolutive” chronic condition. Considerable progress has been made in elucidating the pathophysiological mechanisms of migraine, associated genetic factors that may influence susceptibility to the disease, and functional and anatomical changes during the progression of a migraine attack or the transformation of episodic to chronic migraine. Migraine is a life span neurological disorder that follows an evolutive age-dependent change in its prevalence and even clinical presentations. As a disorder, migraine involves recurrent intense head pain and associated unpleasant symptoms. Migraine attacks evolve over different phases with specific neural mechanisms and symptoms being involved during each phase. In some patients, migraine can be transformed into a chronic form with daily or almost daily headaches. The mechanisms behind this evolutive process remain unknown, but genetic and epigenetic factors, inflammatory processes and central sensitization may play an important role.
Competition between Granule Bound Starch Synthase and Starch Branching Enzyme in Starch Biosynthesis
Authors: Han Huaxin , Yang Chuantian , Zhu Jihui , Zhang Lixia , Bai Yeming , Li Enpeng , Gilbert Robert G. 
Published: December 2019

Abstract
Background
Starch branching enzymes (SBE) and granule-bound starch synthase (GBSS) are two important enzymes for starch biosynthesis. SBE mainly contributes to the formation of side branches, and GBSS mainly contributes for the synthesis of amylose molecules. However, there are still gaps in the understanding of possible interactions between SBE and GBSS.

Results
Nineteen natural rice varieties with amylose contents up to 28% were used. The molecular structure, in the form of the chain-length distribution (CLDs, the distribution of the number of monomer units in each branch) was measured after enzymatic debranching, using fluorophore-assisted carbohydrate electrophoresis for amylopectin and size- exclusion chromatography for amylose. The resulting distributions were fitted to two mathematical models based on the underlying biosynthetic processes, which express the CLDs in terms of parameters reflecting relevant enzyme activities.

Conclusions
Finding statistically valid correlations between the values of these parameters showed that GBSSI and SBEI compete for substrates during rice starch biosynthesis, and synthesis of amylose short chains involves several enzymes including GBSSI, SBE and SSS (soluble starch synthase). Since the amylose CLD is important for a number of functional properties such as digestion rate, this knowledge is potentially useful for developing varieties with improved functional properties.
Alleviating dormancy in Brassica oleracea seeds using NO and KAR1 with ethylene biosynthetic pathway, ROS and antioxidant enzymes modifications
Authors: Sami Abdul , Riaz Muhammad Waheed , Zhou Xiangyu , Zhu Zonghe , Zhou Kejin 
Published: December 2019

AbstractBackgroundSeed dormancy is a prevailing condition in which seeds are unable to germinate, even under favorable environmental conditions. HarvestedBrassica oleracea(Chinese cabbage) seeds are dormant and normally germinate (poorly) at 21 °C. This study investigated the connections between ethylene, nitric oxide (NO), and karrikin 1 (KAR1) in the dormancy release of secondary dormantBrassica oleraceaseeds.ResultsNO and KAR1 were found to induce seed germination, and stimulated the production of ethylene and 1-aminocyclopropane-1-carboxylic acid (ACC), and both ethylene biosynthesis enzyme ACC oxidase (ACO) [1] and ACC synthase (ACS) [2]. In the presence of NO and KAR1, ACS and ACO activity reached maximum levels after 36 and 48 h, respectively. The inhibitor of ethylene 2,5-norbornadiene (NBD) had an adverse effect onBrassica oleraceaseed germination (inhibiting nearly 50% of germination) in the presence of NO and KAR1. The benefits from NO and KAR1 in the germination of secondary dormantBrassica oleraceaseeds were also associated with a marked increase in reactive oxygen species (ROS) (H2O2and O2˙ˉ) and antioxidant enzyme activity at early germination stages. Catalase (CAT) and glutathione reductase (GR) activity increased 2 d and 4 d, respectively, after treatment, while no significant changes were observed in superoxide dismutase (SOD) activity under NO and KAR1 applications. An increase in H2O2and O2˙ˉ levels were observed during the entire incubation period, which increasing ethylene production in the presence of NO and KAR1. Abscisic acid (ABA) contents decreased and glutathione reductase (GA) contents increased in the presence of NO and KAR1. Gene expression studies were carried out with seven ethylene biosynthesis ACC synthases (ACS) genes, two ethylene receptors (ETR) genes and one ACO gene. Our results provide more evidence for the involvement of ethylene in inducing seed germination in the presence of NO and KAR1. Three out of seven ethylene biosynthesis genes (BOACS7, BOACS9andBOACS11), two ethylene receptors (BOETR1andBOETR2) and one ACO gene (BOACO1) were up-regulated in the presence of NO and KAR1.ConclusionConsequently, ACS activity, ACO activity and the expression of different ethylene related genes increased, modified the ROS level, antioxidant enzyme activity, and ethylene biosynthesis pathway and successfully removed (nearly 98%) of the seed dormancy of secondary dormantBrassica olereaceseeds after 7 days of NO and KAR1 application.
Correlation between the Charlson comorbidity index and skeletal muscle mass/physical performance in hospitalized older people potentially suffering from sarcopenia
Authors: Gong Ge , Wan Wenhui , Zhang Xinghu , Liu Yu , Liu Xinhui , Yin Jian 
Published: December 2019

Abstract
Background
Sarcopenia is a decrease in skeletal muscle mass, physical performance, and muscle strength in older people. In this study, we aimed to explore the correlation between comorbidity and skeletal muscle mass and physical performance in older people.

Methods
This retrospective study included 168 subjects. Their medical history, physical function, computed tomography (CT) chest scans, and blood tests for nutrition were evaluated. The patients were divided into two groups: (1) a low muscle mass group and (2) a normal muscle mass group. Multivariate analysis of variance was used to compare multiple sets of mean vectors.

Results
Overall, 72.02% of the subjects had a low skeletal muscle index (SMI) and low gait speed. The patients with low skeletal muscle mass and physical performance were older, had more serious comorbidities, and had longer average hospitalization periods and lower albumin and hemoglobin levels. Subjects with a high Charlson comorbidity index (CCI) were more likely to be in the sarcopenic group than in the non-sarcopenic group. In addition, there was a linear correlation between the CCI and SMI (r = − 0.549, P < 0.05), and between the CCI and gait speed (r = − 0.614, P < 0.05). The area under the curve (AUC) value for low skeletal muscle mass with the CCI was 0.879.

Conclusions
We identified an independent association between comorbidity and skeletal muscle mass/physical performance by researching the correlation between the CCI and SMI/gait speed. Our results suggested that the CCI score may have important clinical diagnostic value for sarcopenia.
The prevalence of obesity in older adults in Iran: a systematic review and meta-analysis
Authors: Vaisi-Raygani Aliakbar , Mohammadi Masoud , Jalali Rostam , Ghobadi Akram , Salari Nader 
Published: December 2019

Abstract
Background
one of the most important age-dependent physiologic alterations in the body composition of older adult people is obesity and overweight, increasing the risk of cardiovascular disease and mortality rate.

Objective
The aim of the present study is to determine the prevalence of obesity in older adults in Iran.

Methods
The present study was conducted via meta-analysis and systematic review method, from March 2000 to October 2018. Subject-related literature was obtained via searches in ScienceDirect, Medline (PubMed), SID, Magiran, Scopus, and Google Scholar databases. Heterogeneity of studies was assessed using the I2 index, and data were analyzed by Comprehensive-Meta analysis software.

Results
In the assessment of 18 studies and 29,943 persons aged over 50 years, the prevalence of obesity in older adults of Iran was 21.4% (95%CI: 26.6–16.9%) based on the meta-analysis. The highest obesity prevalence was obtained in older adults of Babol (Amir Shahr) which was 44.2% (95%CI: 41.1–47.2%) in 2007, while the minimum obesity prevalence was found in older adults of Razavi Khorasan which was 11.3% (95%CI, 10–12.8%) in 2007. Further, as the sample size and the study year increased, the obesity prevalence diminished in older Iranian adults (p < 0.05).

Conclusion
This study suggests that the prevalence of obesity in the older adults of Iran is high. Accordingly, healthcare planners and politicians should consider effective and practical policies to reduce obesity in older adults.
A heterozygous duplication variant of the HOXD13 gene caused synpolydactyly type 1 with variable expressivity in a Chinese family
Authors: Zaib Tahir , Ji Wei , Saleem Komal , Nie Guangchen , Li Chao , Cao Lin , Xu Baijun , Dong Kexian , Yu Hanfei , Hao Xuguang , Xue Yan , Si Shuhan , Jia Xueyuan , Wu Jie , Zhang Xuelong , Guan Rongwei , Ji Guohua , Bai Jing , Chen Feng , Liu Yong , Sun Wenjing , Fu Songbin 
Published: December 2019

Abstract
Background
Synpolydactyly type 1 (SPD1), also known as syndactyly type II, is an autosomal dominant limb deformity generally results in webbing of 3rd and 4th fingers, duplication of 4th or 5th toes. It is most commonly caused by mutation in HOXD13 gene. In this study, a five-generation Chinese family affected with SPD1 disease were collected. We tried to identify the pathogenic variations associated with SPD1 involved in the family.

Methods
We used the whole genome sequencing (WGS) to identify the pathogenic variant in this family which was later confirmed by PCR-Sanger sequencing. The genetic variation were evaluated with the frequencies in the 1000 Genome Project and Exome Aggregation Consortium (ExAC) dataset. The significance of variants were assessed using different mutation predictor softwares like Mutation Taster, PROVEAN and SIFT. The classification of variants was assessed according to American College of Medical Genetics and Genomics (ACMG) guidelines.

Results
Our results showed the mutation of 24-base pair duplication (c.183_206dupAGCGGCGGCTGCGGCGGCGGCGGC) in exon one of HOXD13 in heterozygous form which was predicted to result in eight extra alanine (A) residues in N-terminal domain of HOXD13 protein. The mutation was detected in all affected members of the family.

Conclusion
Based on our mutation analysis of variant c.183_206dupAGCGGCGGCTGCGGCGGCGGCGGC in HOXD13 and its cosegregation in all affected family members, we found this variant as likely pathogenic to this SPD1 family. Our study highlights variable expressivity of HOXD13 mutation. Our results also widen the spectrum of HOXD13 mutation responsible for SPD1.
Factors associated with dietary practice and nutritional status of pregnant women in Dessie town, northeastern Ethiopia: a community-based cross-sectional study
Authors: Diddana Tona Zema 
Published: December 2019

Abstract
Background
Maternal undernutrition is highly prevalent in resource-poor settings. Hence, this study was intended to determine factors associated with the dietary practice and nutritional status of pregnant women in Dessie town, northeastern Ethiopia.

Methods
Community-based cross-sectional study design was employed. Six hundred four (604) pregnant women have participated. A two-stage sampling method was applied to select participants. Socio-demographic and socio-economic data were collected using a structured interviewer-administered questionnaire. The dietary practice was measured using 13 dietary practice questions. Mid upper arm circumference (MUAC) was measured by standard nonstretchable MUAC tape. Data were entered into Epi-Info 7 and exported to SPSS version 20. Binary and multiple logistic regression analysis was conducted. Variables with P < 0.2 in bivariate analysis were entered for multiple logistic regression. At a 95% confidence interval, variable with 푃< 0.05 in multiple logistic regression analysis was considered statistically significant.

Result
About 54.8% of the pregnant women had poor dietary practice and 19.5% were undernourished. First trimester of pregnancy (AOR = 0.46; 95% CI: 0.26, 0.80), no history of illness 2 weeks before data collection date (AOR = 0.42; 95% CI: 0.22, 0.80), poor perceived severity (AOR = 1.64; 95% CI: 1.15, 2.33), poor perceived benefits (AOR = 1.63; 95% CI: 1.14, 2.32) and poor self efficacy (AOR = 4.74; 95% CI: 2.94, 7.65) were significantly associated with poor dietary practice. Not attending antenatal care (ANC) (AOR = 3.46; 95% CI: 2.07, 5.78), illness (AOR = 1.93; 95% CI: 1.10, 3.5), poor dietary diversity (AOR = 5.92; 95% CI: 3.59, 9.76), poor nutrition knowledge (AOR = 3.03; 95% CI: 1.87, 4.92), poor dietary practice (AOR = 3.25; 95% CI: 1.91, 5.54) and poor perceived self efficacy (AOR = 5.59; 95% CI: 3.56, 8.79) were significantly associated (P < 0.05) with undernutrition.

Conclusion
The dietary practice of pregnant women was suboptimal and nutritional status was relatively high. Being in the first trimester of pregnancy and no history illness were negatively associated while poorly perceived severity to malnutrition, poor perceived benefits, and poor self-efficacy were positively associated with the poor dietary practice. Not attending ANC, history of illness, poor dietary diversity, poor nutritional knowledge, poor dietary practice, poorly perceived self-efficacy were positively associated with undernutrition. Government, health extension workers and other concerned bodies should encourage pregnant women to attend ANC, promote health during pregnancy, strength and counsel to improve dietary diversity and practice of good nutrition. They should focus on the perceived belief of dietary behaviors.
Accuracy of multiparametric magnetic resonance imaging for diagnosing prostate Cancer: a systematic review and meta-analysis
Authors: Zhen Liang , Liu Xiaoqiang , Yegang Chen , Yongjiao Yang , Yawei Xu , Jiaqi Kang , Xianhao Wang , Yuxuan Song , Rui Hu , Wei Zhang , Ningjing Ou 
Published: December 2019

Abstract
Background
The application of multiparametric magnetic resonance imaging (mpMRI) for diagnosis of prostate cancer has been recommended by the European Association of Urology (EAU), National Comprehensive Cancer Network (NCCN), and European Society of Urogenital Radiology (ESUR) guidelines. The purpose of this study is to systematically review the literature on assessing the accuracy of mpMRI in patients with suspicion of prostate cancer.

Method
We searched Embase, Pubmed and Cochrane online databases from January 12,000 to October 272,018 to extract articles exploring the possibilities that the pre-biopsy mpMRI can enhance the diagnosis accuracy of prostate cancer. The numbers of true- and false-negative results and true- and false-positive ones were extracted to calculate the corresponding sensitivity and specificity of mpMRI. Study quality was assessed using QUADAS-2 tool. Random effects meta-analysis and a hierarchical summary receiver operating characteristic (HSROC) plot were performed for further study.

Results
After searching, we acquired 3741 articles for reference, of which 29 studies with 8503 participants were eligible for inclusion. MpMRI maintained impressive diagnostic value, the area under the HSROC curve was 0.87 (95%CI,0.84–0.90). The sensitivity and specificity for mpMRI were 0.87 [95%CI, 0.81–0.91] and 0.68 [95%CI,0.56–0.79] respectively. The positive likelihood ratio was 2.73 [95%CI 1.90–3.90]; negative likelihood ratio was 0.19 [95% CI 0.14,-0.27]. The risk of publication bias was negligible with P = 0.96.

Conclusion
Results of the meta-analysis suggest that mpMRI is a sensitive tool to diagnose prostate cancer. However, because of the high heterogeneity existing among the included studies, further studies are needed to apply the results of this meta-analysis in clinic.
Microbial antigens-loaded myeloma cells enhance Th2 cell proliferation and myeloma clonogenicity via Th2–myeloma cell interaction
Authors: Tian Faqing , Lu Bo , Chen Ziren , Liu Junru , Ji Delan , Li Juheng , Tang Meiqin , Zhu Wei , Li Juan 
Published: December 2019

Abstract
Background
Myeloma cells retain B cell functions, considered to be potential antigen presenting cells, yet there is little information regarding promoting Th2 cell proliferation or the direct effects to myeloma on the Th2 cells stimulated by microbial antigens-loaded myeloma cells.

Methods
Mixed lymphocyte reaction was used colorimetric assays via CCK8-kit. Surface molecular expression was performed by flow cytometry, cells sorting using microbeads. The concentrations of cytokines in serum were assessed using an ELISA kit. Clonogenic assay were performed in a methylcellulose culture system. Statistical analysis was assessed using the Student’s t-test or one-way analysis of variance for multiple comparisons test.

Results
The expression of HLA-DR, CD80 and CD40 on RPMI8266 cell membrane surface was upregulated by interaction with interferon-γ and/or Bacillus Calmette-Guerin Vaccine (BCGV). RPMI8266 cells were able to induce the mixed lymphocyte reaction in a dose-dependent fashion. The Th2 ratio induced by RPMI8266 treated by BCGV and interferon-γ (treated-RPMI8266) cells was only slightly greater than by untreated-tumor cells, but the serum IL-4 level secreted by Th2 cells was markedly higher in treated-RPMI8266 cells group. Th2 cells stimulated by treated-myeloma cells could directly promote treated-myeloma cell clonogenicity in a dose-dependent manner. Anti-HLADR IgG2b completely blocked increased of IL-4 secretion by Th2 cells stimulated by treated-myeloma cells, while also blocked enhancing the clonogenicity of treated tumor cells stimulated by MM-Th2 cells.

Conclusions
These results indicate that a novel mechanism of myeloma pathogenesis in myeloma cells could act as an APC to present microbial Ags to Th2 cells, promoting Th2 cell proliferation, consequently facilitating tumor development by close interaction between Th2 myeloma cells. Taken together, the microbial Ag presenting course of MM-Th2-MM interactions—restricted by MHC class-II—may result in tumor development such that all factors involved in the system could have a potential for myeloma therapeutic intervention.
Weight status and obesity-related dietary behaviours among culturally and linguistically diverse (CALD) children in Victoria, Australia
Authors: Scott Breanna , Bolton Kristy A. , Strugnell Claudia , Allender Steven , Marks Jennifer 
Published: December 2019

Abstract
Background
In developed economies, obesity prevalence is high within children from some culturally and linguistically diverse (CALD) backgrounds. This study aims to identify whether CALD groups in Victoria, Australia, are at increased risk of childhood overweight and obesity, and obesity-related dietary behaviours; compared to their non-CALD counterparts.

Methods
Objective anthropometric and self-report dietary behavioural data were collected from 2407 Grade 4 and 6 primary school children (aged 9–12 years). Children were categorised into CALD and non-CALD cultural groups according to the Australian Standard Classification of Languages. Overweight/obesity was defined according to the World Health Organization growth reference standards. Obesity-related dietary behaviour categories included excess consumption of takeaway foods, energy-dense, nutrient-poor snacks and sugar sweetened beverages. T-tests and chi-square tests were performed to identify differences in weight status and dietary behaviours between CALD and non-CALD children. Logistic regression analyses examined the relationship between CALD background, weight status and dietary behaviours.

Results
Middle-Eastern children had a higher overweight/obesity prevalence (53.0%) than non-CALD children (36.7%; p < 0.001). A higher proportion of Middle-Eastern children had excess consumption of takeaway foods (54.9%), energy-dense, nutrient-poor snacks (36.6%) and sugar sweetened beverages (35.4%) compared to non-CALD children (40.4, 27.0 and 25.0%, respectively; p < 0.05). Southeast Asian and African children were 1.58 (95% CI = [1.06, 2.35]) and 1.61 (95% CI = [1.17, 2.21]) times more likely, respectively, to consume takeaway foods at least once per week than non-CALD children.

Conclusions
Disparities in overweight/obesity prevalence and obesity-related dietary behaviours among children in Victoria suggest the need for cultural-specific, tailored prevention and intervention strategies.
Project STARLIT: protocol of a longitudinal study of habitual sleep trajectories, weight gain, and obesity risk behaviors in college students
Authors: Kozak Andrea T. , Pickett Scott M. , Jarrett Nicole L. , Markarian Shaunt A. , Lahar Kari I. , Goldstick Jason E. 
Published: December 2019

Abstract
Background
Obesity in the United States is a serious and preventable health concern. Previous research suggests that habitual short sleep may influence obesity-risk behaviors, such as increased caloric intake, decreased physical activity and increased engagement in sedentary activities (e.g., media consumption, computer usage). Given that existing longitudinal research studies have methodological concerns preventing conclusive interpretations, Project STARLIT was designed to address these limitations and identify future intervention targets.

Methods
A sample of young adults (n = 300) will be recruited during the summer prior to entering college. Participants will be screened for eligibility requirements prior to the inclusion in the Time 1 assessment though phone and in-person interviews. Once enrolled, participants will complete four assessments over a two year period (i.e., approximately 8, 16 and 24 months after Time 1). Each assessment will consist of one week of data collection including both objective (i.e., habitual sleep, physical activity, body fat composition) and subjective (i.e., sleep diary, 24-h food recall, technology use, and sleep-related beliefs/behaviors) measures.

Discussion
Project STARLIT is designed to address methodological concerns of previous research. In addition to clarifying the relationship between habitual short sleep and weight gain among young adults, the proposed study will identify problematic obesity risk behaviors associated with habitual short sleep (e.g., increased caloric intake, physical/sedentary activity). The results will identify prevention or intervention targets related to obesity risk.

Trial registration
ClinicalTrials.gov NCT04100967, 9/23/19, Retrospectively registered.