bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of Key Genes for Controlling Cancer Stem Cell Characteristics in Gastric Cancer
Authors: Huang Chao , Zhao Jiefeng , Ning Zhikun , Huang Jun , Zhu Zhengming 
Published: June 2020

Abstract
Background

Self-renewal of gastric cancer stem cells (GCSCs) is considered to be the underlying cause of the metastasis, drug resistance and recurrence of gastric cancer (GC). The purpose of this study was to characterize the expression of stem cell-related genes in GC.
Methods

The RNA sequencing results and clinical data for gastric adenoma and adenocarcinoma samples were obtained from the Cancer Genome Atlas (TCGA) database, and the results of the GC mRNA expression-based stemness index (mRNAsi) were analyzed. Weighted gene coexpression network analysis (WGCNA) was then used to find modules of interest and their key genes. Survival analysis of key genes was performed using the online tool Kaplan-Meier Plotter, and the online database Oncomine was used to assess the expression of key genes in GC.
Results

mRNAsi was significantly upregulated in GC tissues compared to normal gastric tissues (p < 0.0001). A total of 16 modules were obtained from the gene coexpression network; the brown module was most positively correlated with mRNAsi. Sixteen key genes (BUB1, BUB1B, NCAPH, KIF14, RACGAP1, RAD54L, TPX2, KIF15, KIF18B, CENPF, TTK, KIF4A, SGOL2, PLK4, XRCC2 and C1orf112) were identified in the brown module. Survival analysis and Oncomine analysis revealed that the prognosis of patients with GC and the expression of three genes (RAD54L, TPX2 and XRCC2) were consistently related.
Conclusion

Sixteen key genes play an important role in the maintenance of GCSCs. RAD54L, TPX2 and XRCC2 are the most likely therapeutic targets for inhibiting the stemness characteristics of GC cells.
Factors related to Juvenile Idiopathic Arthritis Complicated with Hyperuricemia
Authors: wu ling , Di Yazhen , Chen Yuanling , Li Yunyan , Wang Jiapei 
Published: June 2020

Abstract
Background

Clinical findings reported that some cases of Juvenile Idiopathic Arthritis(JIA) gradually suffer from hyperuricemia(HUA) during the course of follow-up, followed by the detection of high urate crystal, gout, renal impairment, and other manifestations. And thoes patients would influence the prognosis.
Methods

This was a retrospective study of 60 patients diagnosed with JIA between October 2016 and March 2019 and followed up for > 1 year. Single-factor analyses of the clinical data, laboratory data, and the special drug used for JIA complicated with hyperuricemia (Group A, n = 18) and JIA without hyperuricemia (Group B, n = 42) were conducted.
Results

Comparison between Groups A and B revealed differences in sex; disease course; high disease activity; diastolic pressure; the levels of serum albumin (ALB), alanine aminotransferase (ALT), aspartate transaminase (AST), triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), glucose (GLU), and urea nitrogen (BUN); NSAIDS application, systemic glucocorticoid application, MTX application, and tumor necrosis factor-alpha (TNF-α) inhibitors application were not statistically significant (P > 0.05). However, the differences in age, active sacroiliitis, body mass index (BMI), systolic pressure, serum creatinine (Scr) level, and salicylazosulfapyridine (SASP) application showed a statistical significance (P < 0.05).
Conclusions

JIA patients were obese ,with high systolic pressure, and after SASP treatment will be more likely to be complicated with hyperuricemia.
The intervention of S1PR1/STAT3 signaling pathway by inhibiting the expression of STAT3 attenuates the valvular damage due to rheumatic heart disease
Authors: Xian Shenglin , Chen Ang , Wu Yunjiao , Wen Hong , Lu Chuanghong , Wen Jianlin , Huang Feng , Zeng Zhiyu 
Published: June 2020

Abstract
Background

Rheumatic heart disease (RHD) is a social health problem that affects many patients every year, but its pathogenesis is still unclear. Recent studies have found that the sphingosine 1-phosphate receptor 1 (S1PR1)/signal transducer and activator of transcription 3 (STAT3) signaling pathway is activated during the development of valvular damage caused by RHD. STAT3 plays an important role in the differentiation of CD4 + T cells into T helper 17 (Th17) cells, and Th17 cell-related cytokines are involved in the development of RHD. In this work, we investigated whether altering the S1PR1/STAT3 signaling pathway by inhibiting the expression of STAT3 attenuates valvular damage due to RHD.
Methods

Inactivated Group A streptococci (GAS) and complete Freund’s adjuvant (CFA) were used to establish the RHD rat model. STAT3-small interfering RNA (STAT3-siRNA) was used to directly inhibit the expression of STAT3 in the heart. Histological examination was used to evaluate the degree of inflammation and fibrosis in valve tissues. Enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry were used to detect the levels of interleukin (IL)-6 and IL-17 in serum and valve tissue. Reverse transcription-quantitative PCR (RT-qPCR) was used to detect the mRNA expression of S1PR1 and STAT3. Western blotting (WB) and immunohistochemistry were used to detect the protein expression of S1PR1, STAT3, phosphorylated (p-) STAT3, retinoic acid-related orphan receptor gamma T (RORγt).
Results

The expression of S1PR1 in valve tissues of RHD model rats was decreased. The degree of valvular inflammation and fibrosis of RHD model rats was increased. The levels of IL-6 and IL-17 in valves and serum of RHD model rats were increased as well as an increase of p-STAT3 in valve tissues. Inhibition of STAT3 by STAT3-siRNA decreased STAT3 expression and reduced the total amount of p-STAT3, resulting in decreased expression of IL-6, IL-17 and RORγt in valves and serum and reduced valvular inflammatory response and fibrosis.
Conclusions

These results suggest that inhibiting the expression of STAT3 in the heart may reduce the valvular damage caused by rheumatic heart disease.
The Horse Gallops But So Does the Zebra! A Case Report: Peripheral Ulcerative Keratitis
Authors: belghmaidi sarah , belgadi soumia , Hajji ibtissam , Moutaouakil abdeljalil 
Published: June 2020

Abstract
Introduction: Peripheral ulcerative keratitis (PUK) is an aggressive, potentially sight-threatening cause for peripheral corneal thinningCase report: A 43-year-old woman presented with bilateral tearing and ocular pain persisting for 7 months. Visual acuity was 6/6 in both eyes. On biomicroscopic examination, presence of crescent shaped corneal ulcer with perilesional haziness. A tear film study demonstrated decreased Schirmer test results (5mm in both eyes) and tear film break-up time (5 sec in both eyes). The interrogation and the patient revealed he had recurrent skin lesions in both legs with chronic itching, and recurrent oral and genital ulcers healing spontaneously. The diagnosis of behcet disease was proposed.Conclusion: Collaborative management of ophthalmic patients having systemic illness along with internists improves overall outcomes and prevents morbidity in such patients.
Revelation of critical genes and pathways involved in hepatocellular carcinoma (HCC) and phylogenetic tree construction of CDK1 protein family An integrated multi-omics bioinformatics analysis
Authors: Zhou Jingyang , Chen Ziyi , Cao Weiwei , Chen Weiqi , Zhou Wuyuan 
Published: June 2020

Abstract
Background

Hepatocellular carcinoma (HCC) is one of the leading and fastest increasing causes of death in the world. It primarily happens in the people who have been exposed to high-risk factors for a long time, such as the hepatitis B virus, hepatitis C virus, etc. Some similar analysis on HCC were published years ago, and results need updating.
Methods

In this study. We analyzed the gene expression pattern obtained from the GEO database in healthy tissue and tumor tissue and identified differentially expressed genes (DEGs), then GO and KEGG pathway analyses were performed subsequently on DEGs. We also used the protein-protein interaction (PPI) network drawn from STRING to find hub genes and analyzed their effects on prognosis and expression level in different stages of HCC from GEPIA. Furthermore, we analyzed the CDK1 in different species and established the phylogenetic tree of the CDK1 family by the MEGA-X (version 5) according to the amino acid sequence of CDK1 in different species.
Results

Among all DEGs identified in this study, the CDK1 is the most statically significant hub gene in the perspective of prognosis and differential ex pression. The expression level of CDK1 varies in different stages of HCC. The expression level reaches the highest in stage two and three and reaches the lowest in Stage four. And the human CDK1 gene and ape-like CDK1 gene subtype 5 may be homologous genes. However, human CDK1 gene and the gene encoding human CDK1 protein A chain may be heterozygous genes.
Conclusion

CDK1 is a gene with great significance in the diagnosis and prognosis of HCC, more studies that investigate deeply about the CDK1 is needed in the future.
TRAPPC11-Related Muscular Dystrophy with Hypoglycosylation of Alpha-Dystroglycan in Skeletal Muscle and Brain
Authors: Munot Pinki , McCrea Nadine , Torelli Silvia , Manzur Adnan , Sewry Caroline , Chambers Darren , Feng Lucy , Ala Pierpaolo , Zaharieva Irina , Ragge Nicola , Roper Helen , Marton Tamas , Cox Phil , Milev Miroslav , Sacher Michael , Liang Wen-Chen , Maruyama Shinsuke , Nishino Ichizo , Phadke Rahul , Muntoni Francesco 
Published: June 2020

Abstract
Background: TRAPPC11, a subunit of the transport protein particle (TRAPP) complex is important for complex integrity and anterograde membrane transport from the endoplasmic reticulum (ER) to the ER-Golgi intermediate compartment. Several individuals with TRAPPC11 mutations have been reported with muscle weakness and other features including brain, liver, skeletal and eye involvement. A detailed analysis of brain and muscle biopsies will further our understanding of the presentation and etiology of TRAPPC11-disease.Methods: We describe five cases of early-onset TRAPPC11–related muscular dystrophy with a systematic review of muscle pathology and post-mortem brain pathology findings in one individual, and membrane trafficking assays in another.Results: All affected individuals presented in infancy with muscle weakness, motor delay and elevated serum creatine kinase (CK). Additional features included cataracts, liver disease, intellectual disability, cardiomyopathy, movement disorder, and structural brain abnormalities. Muscle pathology in all five revealed dystrophic changes, universal hypoglycosylation of alpha-dystroglycan and variably reduced dystrophin-associated complex proteins. Membrane trafficking assays showed defective Golgi trafficking in one individual. Neuropathological examination of one individual revealed cerebellar atrophy, granule cell hypoplasia, Purkinje cell (PC) loss and dendritic neurodegeneration, reduced alpha-dystroglycan (IIH6) expression in PC and dentate neurons, and absence of neuronal migration defects.Conclusions: This report suggests that recessive mutations in TRAPPC11 are linked to muscular dystrophies with hypoglycosylation of alpha-dystroglycan. The structural brain involvement that we document for the first time resembles the pathology previously reported in N-linked congenital disorders of glycosylation (CDG) such as PMM2-CDG, suggesting defects in multiple glycosylation pathways in this condition.
IL-22 hinders antiviral T cell responses and exacerbates neurological disorders in ZIKV-infected immunocompetent neonatal mice
Authors: Liang Yuejin , Yi Panpan , Ru Wenjuan , Jie Zuliang , Wang Hui , Ghanayem Tamer , Wang Xiaofang , Alamer Edrous , Liu Jinjun , Hu Haitao , Soong Lynn , Cai Jiyang , Sun Jiaren 
Published: June 2020

Abstract
Background
The Zika virus (ZIKV) outbreak that occurred in multiple countries was linked to increased risk of neurological disorders and congenital defects. However, host immunity and immune-mediated pathogenesis in ZIKV infection are not well understood. Interleukin-22 (IL-22) is a crucial cytokine for regulating host immunity in infectious diseases. Whether IL-22 plays a role in ZIKV infection is unknown.
Methods
The cellular source of IL-22 was identified in IFNAR−/− mice and WT neonatal mice during ZIKV infection. To determine the role of IL-22, we challenged 1-day-old wild-type (WT) and IL-22−/− mice with ZIKV and monitored clinical manifestations. Glial cell activation in the brain was assessed by confocal imaging. ZIKV-specific CD8+ T cell responses in both the spleen and brain were analyzed by flow cytometry. In addition, we infected mouse primary astrocytes in vitro, and characterized the reactive astrocyte phenotype. Human glial cell line was also infected with ZIKV in the presence of IL-22, followed by the evaluation of cell proliferation, cytokine expression and viral loads.
Results
We found that γδ T cells were the main source of IL-22 during ZIKV infection in both the spleen and brain. WT mice began to develop weight loss, staggered steps, bilateral hind limb paralysis, weakness at 10 days post-infection (dpi), and ultimately succumbed to infection at 16–19 dpi. Surprisingly, IL-22 deficiency lessened weight loss, moderated the systemic inflammatory response, and greatly reduced the incidence of neurological disorders and mortality. ZIKV infection facilitated a neurotoxic polarization of A1-prone astrocytes in vitro. Additional analysis demonstrated that the absence of IL-22 resulted in reduced activation of microglia and astrocytes in the cortex. Although IL-22 displayed a marginal effect on glial cells in vitro, IL-22−/− mice mounted more vigorous ZIKV-specific CD8+ T cell responses, which led to a more effective control of ZIKV in the brain.
Conclusions
Our data revealed a pathogenic role of IL-22 in ZIKV encephalitis.
Hearing loss as a newly recognized symptom of GSD type I. A clinical report of four unrelated Polish patients.
Authors: Iwanicka-Pronicka Katarzyna , Trubicka Joanna , Pronicki Maciej , Szymanska Edyta , Ciara Elżbieta , Rokicki Dariusz , Wortmann Saskia 
Published: June 2020

Abstract
Background: Glycogen storage disease (GSD) type Ia and Ib is the most severe type of all hepatic GSDs. It presents with serious liver and metabolic complications, as well as in type Ib with severe infections due to neutropenia. So far, the hearing impairment has not been reported in these patients. Hearing status was assessed in a group counting 40 probands with GSDI (20 patients with each subtype). Hearing loss was detected in four patients. They did not pass newborn hearing screening. Due to the development of severe metabolic complications in infancy, audiological diagnosis was postponed until the early school age, when speech and language delay was striking.Hearing tests revealed the bilateral sensorineural hearing impairment with the audiograms presenting ski-slope curves in all patients. Next-generation sequencing (NGS) using gene panel: TruSight OneTM Sequencing Panel Kit (Illumina, San Diego, CA, USA) or whole-exome sequencing (WES) using SureSelect Human All Exon Kit (Agilent, 60mb V6) were performed in order to find possible genetic background of auditory dysfunctions in these patients.Results: Hearing impairment seems to affect about10% GSDI patients. Molecular analysishas revealed the common homozygous pathogenic variant in G6PC in two GSDIa probands. Common pathogenic variant in SLC37A4 gene was identifiedin one GSDIb patient, whereas the second case was found to be a carrier of one common and one rare pathogenic variant in SLC37A4 gene ( in trans ). No change in the genes involved in auditory pathway dysfunction was found, although the patients displayed the same audiological characteristics.Conclusions: Sensorineural hearing lossappears to be a clinical complication of GSDI encountered in approximately one out of ten cases. Carefulmonitoring of auditory functions is recommended among GSDI patients, especially in prelingulal period. Appropriate early detection and intervention (fitting with hearing aids) prevents potential speech and language impairment.
Effect of Being Housewife and Counseling During Antenatal Care on Exclusive Breastfeeding Practice Among Mothers with Less than Two Years of Age in Northeast Ethiopia, 2019
Authors: Ayele Wolde 
Published: June 2020

Abstract
Background Exclusive breastfeeding is the practice of a woman feeding an infant for six months, which has an irreplaceable benefit. However, the practice is still low with salient factors in a different place. Therefore, this study was aimed to assess exclusive breastfeeding practice and associated factors among mothers of having children less than two-years of age in Northeastern Ethiopia, 2019.Methods A community-based cross-sectional study was conducted with a sample size of 423 in Northeast Ethiopia from March 12 to December 18, 2019. An interviewer-administered questionnaire was used to collect the data. The binary logistic regression model was used to determine the association between dependent and independent variables.Result The prevalence of exclusive breastfeeding practice was 75.5%(95%CI:73.5,81.5%). Married mothers 2.57(1.688, 5.654), mothers with antenatal care follow up 4.11(2.662,11.172), mothers delivered at health institution 4.07(2.992,10.722), and mothers counseled during antenatal care 1.96(1.124,4.732) had a positive association, while mothers with unable to read and write educational status 0.11(0.068,0.992) and employed mothers 0.22(0.169,0.561) were the variable that had a negative association with exclusive breastfeeding practice.Conclusion The prevalence of exclusive breastfeeding practice was relatively good. Marital status, educational status, occupation, antenatal care service, place of birth, and counseling of mothers during ANC were the independent predictors of exclusive breastfeeding practice.
Relationship between Obesity and Eating Rate: Analyzing the Korean Community Health Survey Data
Authors: Jo Heui Sug , Jung Su Mi , Dronina Yuliya 
Published: June 2020

Abstract
Background: The obesity rate has increased in South Korea as a result of changing dietary habits and decreasing physical activity. This study aimed to evaluate the relationships between eating rate and obesity.Methods: Multiple logistic regression analyses were conducted using raw data of the Korean Community Health Survey 2017 for the Gangwon Province and nationwide data to evaluate the relationships between eating rate and obesity.Results: Among men, the major factor affecting obesity was an eating rate of ≤ 20 min (odds ratio = 1.17 (1.03, 1.33); P < 0.01). In case of women, factors affecting obesity were an eating rate of ≤ 20 min (odds ratio = 1.15 (1.02, 1.29); P < 0.05), and irregular mealtimes (odds ratio = 1.16 (1.03, 1.30); P < 0.05).Conclusion: Implementing simple strategies to improve dietary and lifestyle habits may assist in weight and obesity management. These findings may provide useful strategies for future developing a culture-oriented approach to obesity management.
Individual Pain Sensitivity is Associated With Resting-state Cortical Activities in Healthy Individuals but Not in Patients With Migraine: A Magnetoencephalography Study
Authors: Hsiao Fu-Jung , Chen Wei-Ta , Liu Hung-Yu , Wang Yen-Feng , Chen Shih-Pin , Lai Kuan-Lin , Pan Li-Ling Hope , Wang Shuu-Jiun 
Published: June 2020

Abstract
Background:Pain sensitivitymay determine the risk, severity, prognosis, and efficacy of treatment of clinical pain. Magnetic resonance imaging studies have linked thermal pain sensitivity to changes in brain structure. However, the neural correlates ofmechanical pain sensitivityremain to be clarified through investigation ofdirect neural activitieson theresting-state corticaloscillation and synchrony.Methods:We recorded the resting-state magnetoencephalographic (MEG) activities of 27 healthy individuals and 30 patients with episodic migraine (EM)and analyzed the source-based oscillatory powers and functional connectivity at 2 to 59 Hz in pain-related cortical regions, which are thebilateral anterior cingulate cortex (ACC), medial orbitofrontal (MOF) cortex, lateral orbitofrontal (LOF) cortex, insula cortex, primary somatosensory cortex (SI), primary motor cortex (MI), and posterior cingulate cortex (PCC). The mechanical punctate pain threshold (MPPT) was obtained at the supraorbital area (the first branch of the trigeminal nerve dermatome,V1) and the forearm (the first thoracic nerve dermatome,T1) and further correlated with MEG measures.Results:TheMPPTis inversely correlated with the resting-state relative powers of gamma oscillation in healthy individuals (all corrected P<0.05). Specifically, inverse correlation was noted between the MPPT at V1 and gamma powersin the bilateral insula (r= −0.592 [left] and −0.529 [right]), PCC (r= −0.619 and −0.541) and MI (r = −0.497 and −0.549)and between the MPPT at T1 and powers in the left PCC (r = −0.561) and bilateral MI (r = −0.509 and −0.520). Furthermore,resting-state functional connectivityat the delta to beta bands, especially between frontal (MOF, ACC, LOF, and MI), parietal (PCC),andsensorimotor (bilateral SI and MI)regions,showed a positive correlation with the MPPTat V1 and T1(all corrected P<0.05). By contrast, in patients with EM, the MPPT was not associated with resting-state cortical activities.Conclusions:Pain sensitivity in healthy individuals is associated with the resting-state gamma oscillation and functional connectivity in pain-related cortical regions. Further studies must be conducted in a large population to confirm whether resting-state cortical activities can be an objective measurement of pain sensitivity in individuals without clinical pain.
Association of livestock ownership and household dietary quality: results from a cross-sectional survey from rural India
Authors: Pradyumna Adithya , Winkler Mirko S. , Utzinger Jürg , Farnham Andrea 
Published: June 2020

Abstract
Background
Studies from India and Africa found that the impact of dairy animal ownership on household nutrition varied greatly, depending on the social and geographic context. There is a paucity of studies from semi-arid rural districts in southern India where livestock rearing is being promoted to improve household income and nutrition as part of watershed development (WSD) projects. The purpose of this study was to (i) assess socio-demographic factors and health determinants of households based on dairy animal ownership; (ii) determine the correlation between milk consumption and other sources of nutrition; and (iii) examine the association between livestock ownership and milk consumption.
Methods
We collected data from an exhaustive household survey in four study villages of Kolar district, applying a cross-sectional design. Kendall’s rank correlation coefficient was employed to determine the correlation between milk consumption and other dietary variables. Multivariable logistic regression was used to describe the relationship between dairy animal ownership and household milk consumption.
Results
Full data records were available from 195 households. Less than half of the households (43.1%) owned dairy animals. Households owning dairy animals more often had access to irrigation (58.3% vs 25.2%) and were less often woman-headed (2.4% vs 22.5%). Household milk consumption was significantly correlated with consumption of vegetable variety, egg and meat (all p-values < 0.05). After adjusting for multiple confounders, the odds of dairy animal-owning households consuming milk was 2.11 (95% confidence interval 0.85, 5.45) compared to households not owning dairy animals.
Conclusion
While dairy animal ownership was found to be associated with improved dietary quality, larger households were in a better position to adopt dairy animals, which, in turn, might contribute to better household nutrition. Our study added to the evidence on health inequity in semi-arid rural areas and the importance of socio-geographical context in determining nutritional impacts of developmental interventions.