bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of nusinersen after one year of treatment in 123 children with SMA type 1 or 2: a French real-life observational study
Authors: Audic Frédérique , de la Banda Marta Gomez Garcia , Bernoux Delphine , Ramirez-Garcia Paola , Durigneux Julien , Barnerias Christine , Isapof Arnaud , Cuisset Jean-Marie , Cances Claude , Richelme Christian , Vuillerot Carole , Laugel Vincent , Ropars Juliette , Altuzarra Cécilia , Espil-Taris Caroline , Walther-Louvier Ulrike , Sabouraud Pascal , Chouchane Mondher , Vanhulle Catherine , Trommsdorff Valérie , Pervillé Anne , Testard Hervé , Lagrue Emmanuelle , Sarret Catherine , Avice Anne-Laude , Beze-Beyrie Pierre , Pauly Vanessa , Quijano-Roy Susana , Chabrol Brigitte , Desguerre Isabelle 
Published: June 2020

Abstract
Background
Spinal muscular atrophy (SMA) is an autosomal recessive neuromuscular disorder characterized by degeneration of the anterior horn cells of the spinal cord. Nusinersen has been covered by public healthcare in France since May 2017. The aim of this article is to report results after 1 year of treatment with intrathecal nusinersen in children with SMA types 1 and 2 in France. Comparisons between treatment onset (T0) and after 1 year of treatment (Y1) were made in terms of motor function and need for nutritional and ventilatory support. Motor development milestone achievements were evaluated using the modified Hammersmith Infant Neurologic Examination–Part 2 (HINE-2) for patients under 2 years of age and Motor Function Measure (MFM) scores for patients over 2 years of age.

Results
Data on 204 SMA patients (type 1 or 2) were retrospectively collected from the 23 French centers for neuromuscular diseases. One hundred and twenty three patients had been treated for at least 1 year and were included, 34 of whom were classified as type 1 (10 as type 1a/b and 24 as type 1c) and 89 as type 2. Survival motor Neuron 2 (SMN2) copy numbers were available for all but 6 patients.
Patients under 2 years of age (n = 30), had significantly higher HINE-2 scores at year 1 than at treatment onset but used more nutritional and ventilatory support. The 68 patients over 2 years of age evaluated with the Motor Function Measure test had significantly higher overall scores after 1 year, indicating that their motor function had improved. The scores were higher in the axial and proximal motor function (D2) and distal motor function (D3) parts of the MFM scale, but there was no significant difference for standing and transfer scores (D1). No child in either of the two groups achieved walking.

Conclusion
Nusinersen offers life-changing benefits for children with SMA, particularly those with more severe forms of the disorder. Caregiver assessments are positive. Nevertheless, patients remain severely disabled and still require intensive support care. This new treatment raises new ethical challenges.
Ultrasonographic documentation of type-3 abomasal ulcer in a cow with left displacement of the abomasum
Authors: Gerspach Christian , Oschlies Carina , Kuratli Jasmin , Braun Ueli 
Published: June 2020

Abstract
Background
Ultrasonographic documentation of perforated abomasal ulcer has not been published till now. This report describes the clinical, ultrasonographic and postmortem findings in a Jersey cow with type-3 abomasal ulcer and left displacement of the abomasum (LDA).

Case report
The main clinical findings were abnormal demeanour, rumen atony and tympany, positive foreign body tests, positive auscultation and simultaneous ballottement on the left side, abdominal guarding and loss of negative pressure in the abdominal cavity. The tentative diagnosis was peritonitis and LDA. Abdominal ultrasonography produced images typical of LDA, and in one location between the abdominal wall and abomasum there was a layer of fibrin, a fibrin clot, a break in the abomasal contour, suggestive of a perforated ulcer, and partial obstruction of this gap with fibrin. The diagnosis of perforated abomasal ulcer with subsequent peritonitis was confirmed during postmortem examination.

Conclusions
The examination of this case shows that under certain circumstances, ultrasonographic imaging of a perforated abomasal ulcer in a cow is possible. Antemortem diagnosis of type-3 abomasal ulcer is preferable to relying on exploratory laparotomy and/or post-mortem examination.
The BDNF-FoxO1 Axis in the medial prefrontal cortex modulates depressive-like behaviors induced by chronic unpredictable stress in postpartum female mice
Authors: Liu Jing , Meng Fantao , Dai Juanjuan , Wu Min , Wang Wentao , Liu Cuilan , Zhao Di , Wang Hongcai , Zhang Jingyan , Li Chen 
Published: June 2020

AbstractPostpartum depression (PPD) is a serious psychiatric disorder, affecting not only the childbearing women but also the health of their offsprings. The brain-derived neurotrophic factor (Bdnf) gene is an important target gene for the study of depression and antidepressant therapy. FoxO1, belonging to the FoxO subfamily is involved in the development of major depressive disorders. However, the role of BDNF and its functional brain regions involved in PPD remains unknown. Here, we report that chronic unpredictable stress (CUS) can produce depression-associated behaviors in postpartum female mice. CUS can decrease totalBdnfmRNA and exon specific mRNAs in the medial prefrontal cortex (mPFC), accompanied by reduced protein levels, that were correlated with depression-related behaviors. Moreover, postpartum, not virgin female mice showed increased susceptibility to subthreshold stress-induced depression-related behaviors. Selective deletion of BDNF in the mPFC induced anhedonia as indicated by reduced sucrose preference and increased latency to food in the novelty suppressed food test in postpartum, but not in virgin female mice. Furthermore, we found that FoxO1 is also decreased in CUS-treated postpartum female mice with a significant correlation with depression-related behaviors. BDNF-specific knockout in the mPFC decreased FoxO1 expression in female mice. Our results indicate that the BDNF-FoxO1 axis in mPFC can regulate depression-related behaviors and stress vulnerability in postpartum female mice.
Extended doxycycline treatment versus salpingectomy in the management of patients with hydrosalpinx undergoing IVF-ET
Authors: Fouda Usama M. , Elshaer Hesham S. , Youssef Mohamed A. , Darweesh Fatma F. 
Published: June 2020

Abstract
Background
The aim of this study was to determine whether the treatment with doxycycline before and after oocyte retrieval is as effective as salpingectomy in minimizing the detrimental effect of hydrosalpinx on the outcomes of IVF-ET.

Methods
A retrospective analysis was done for the outcomes of the IVF-ET cycles of patients with hydrosalpinx who underwent laparoscopic salpingectomy prior to IVF cycle (n = 260) or were treated with extended doxycycline treatment during the IVF cycle (n = 45). In doxycycline group, doxycycline (100 mg twice daily) was started 1 week before anticipated oocyte retrieval and was continued for 1 week after oocyte retrieval. In salpingectomy group, the mesosalpinx was coagulated as close as possible to the fallopian tube.

Results
The implantation, clinical pregnancy, ongoing pregnancy and live birth rates were significantly higher in the salpingectomy group (20.87% Vs. 9.91%, P value =0.007, 44.62% Vs. 20%, P value = 0.002, 39.62% Vs. 17.78%, P value = 0.005 and 37.31% Vs. 15.56%, P value = 0.005 respectively).

Conclusion
Salpingectomy is more effective than extended doxycycline treatment in improving the outcomes of IVF-ET in patients with hydrosalpinx undergoing IVF-ET. Further, larger well designed randomized controlled trials should be conducted to confirm the findings of this study.
The interaction between arterial oxygenation and carbon dioxide and hospital mortality following out of hospital cardiac arrest: a cohort study
Authors: McGuigan Peter J. , Shankar-Hari Manu , Harrison David A. , Laffey John G. , McAuley Danny F. 
Published: June 2020

AbstractBackgroundOutcomes following out of hospital cardiac arrest (OHCA) are poor. The optimal arterial oxygen and carbon dioxide (PaCO2) levels for managing patients following OHCA are unknown. We hypothesized that abnormalities in arterial oxygenation (PaO2/FiO2ratio or PaO2) and PaCO2would be associated with hospital mortality following OHCA. We hypothesized that PaCO2would significantly modify the oxygenation–mortality relationship.MethodsThis was an observational cohort study using data from OHCA survivors admitted to adult critical care units in England, Wales and Northern Ireland from 2011 to 2018. Logistic regression analyses were performed to assess the relationship between hospital mortality and oxygenation and PaCO2.ResultsThe analysis included 23,625 patients. In comparison with patients with a PaO2/FiO2 > 300 mmHg, those with a PaO2/FiO2 ≤ 100 mmHg had higher mortality (adjusted OR, 1.79; 95% CI, 1.48 to 2.15;P < 0.001). In comparison to hyperoxemia (PaO2 > 100 mmHg), patients with hypoxemia (PaO2 < 60 mmHg) had higher mortality (adjusted OR, 1.34; 95% CI, 1.10 to 1.65;P = 0.004). In comparison with normocapnia, hypercapnia was associated with lower mortality. Hypocapnia (PaCO2 ≤ 35 mmHg) was associated with higher mortality (adjusted OR, 1.91; 95% CI, 1.63 to 2.24;P < 0.001). PaCO2modified the PaO2/FiO2–mortality and PaO2–mortality relationships, though these relationships were complex. Patients who were both hyperoxic and hypercapnic had the lowest mortality.ConclusionsLow PaO2/FiO2ratio, hypoxemia and hypocapnia are associated with higher mortality following OHCA. PaCO2modifies the relationship between oxygenation and mortality following OHCA; future studies examining this interaction are required.
An outbreak of bovine babesiosis in February, 2019, triggered by above average winter temperatures in southern England and co-infection with Babesia divergens and Anaplasma phagocytophilum
Authors: Johnson Nicholas , Paul Phipps L. , McFadzean Harriet , Barlow Alex M. 
Published: June 2020

Abstract
Background
Bovine babesiosis, commonly known as redwater fever, is a sporadic tick-borne disease in the United Kingdom. Outbreaks occur during the spring, summer and autumn months when ticks are active. This study reports the findings of an investigation of an outbreak of bovine babesiosis during the winter month of February, 2019.

Methods
DNA from blood, organ and tick samples taken from affected cattle were tested for the presence of piroplasm and Anaplasma phagocytophilum DNA using PCRs directed to the 18S rRNA gene and msp2 gene respectively. The species of piroplasm was confirmed by sequencing.

Results
Babesia divergens DNA was detected in the blood of five cattle displaying clinical signs of babesiosis within a herd of twenty. This parasite was also detected in three of ten ticks removed from one of the affected cattle. In addition, A. phagocytophilum was detected in three cattle tested and two of ten of the ticks.

Conclusions
An outbreak of bovine babesiosis during February is unusual as the tick vector, Ixodes ricinus, does not generally become active until temperatures rise later in the year. February of 2019 was unusual as average temperatures during the first week of the month reached over 10 °C, well above historical averages that are typically below 5 °C, and a temperature at which ticks can become active. This unusual weather event is likely to have triggered tick questing, that combined with a co-infection with two tick-borne pathogens caused the severe outbreak of disease.
First epidemiological survey of Angiostrongylus vasorum in domestic dogs from Spain
Authors: Carretón Elena , Morchón Rodrigo , Falcón-Cordón Yaiza , Matos Jorge , Costa-Rodríguez Noelia , Montoya-Alonso José A. 
Published: June 2020

AbstractBackgroundAngiostrongylus vasorumis the causative agent of canine angiostrongylosis, a disease that mainly affects domestic dogs and other wild carnivores. In Europe, the number of infected individuals is increasing, being located in central and southern countries. In Spain, several studies have reported high prevalence ofA. vasorumin wild animals. However, there are no studies addressing the current situation of the disease or its distribution in domestic dogs, and reports from veterinary personnel are very limited. Considering these facts, the objective of the present study was to evaluate the prevalence ofA. vasorumin different areas of Spain.MethodsBetween November 2018 and October 2019, blood was sampled from a total of 2024 domestic dogs from six zones of Spain with a climate that favours the establishment of the disease, where all dogs included in the study lived outdoors or had regular access to areas with vegetation and none had travelled outside the study area of interest in the past year. Details about their sex and age were collected. All dogs were tested for the presence ofA. vasorumcirculating antigens using Angio DetectTM.ResultsThe overall prevalence of canine angiostrongylosis in the studied areas of Spain was 1.73%. No differences in overall prevalence were found between males and females, neither between age groups. Regarding eco-epidemiological areas, the highest prevalence was recorded in the zones located in the north and northwest of Spain (1.86–2.74%), which correspond to the wetter climates and most abundant vegetation, and the lowest prevalence was detected in the zones located in the center and west of Spain (0.93–0.99%).ConclusionsOur data suggest that angiostrongylosis is present in Spain in domestic dogs where previously infected wild animals existed or where climatic conditions are favourable for the establishment of the disease.
Low food and nutrition literacy (FNLIT): a barrier to dietary diversity and nutrient adequacy in school age children
Authors: Doustmohammadian Azam , Omidvar Nasrin , Keshavarz-Mohammadi Nastaran , Eini-Zinab Hassan , Amini Maryam , Abdollahi Morteza , Amirhamidi Zeinab , Haidari Homa 
Published: June 2020

Abstract
Objective
This study aims to assess the relationship between Food and Nutrition Literacy (FNLIT) and dietary diversity score (DDS); FNLIT and nutrient adequacy (NAR%, MAR%) in school-age children in Iran.

Results
This cross-sectional study was undertaken on 803 primary school students in Tehran, Iran. Socio-economic, as well as three 24-h dietary recalls were collected through interviewing students and their mothers/caregivers. FNLIT was measured by a self-administered locally designed and validated questionnaire. Low level of FFNL was significantly associated with higher odds of low DDS (OR = 2.19, 95% CI 1.32–3.62), the first tertile of fruit diversity score (OR = 3.88, 95% CI 2.14–6.99), and the first tertile of dairy diversity score (OR = 9.60, 95% CI 2.07–44.58). Low level of IFNL was significantly associated with probability of lower meat diversity score (OR = 1.73, 95% CI 1.07–2.81). Low level of FLL was also significantly associated with probability of lower DDS (OR = 1.81, 95% CI 1.11–2.94), dairy diversity score (OR = 2.01, 95% CI 1.02–3.98), and meat diversity score (OR = 2.14, 95% CI 1.32–3.45).Low FNLIT and its subscales were associated with higher odds of low level of NAR of protein, calcium, vitamin B3, B6, B9, as well as the probability of lower level of MAR.
Infinitely many positive solutions for a nonlocal problem with competing potentials
Authors: Yang Jing 
Published: June 2020

AbstractThe present paper deals with a class of nonlocal problems. Under some suitable assumptions on the decay rate of the coefficients, we derive the existence of infinitely many positive solutions to the problem by applying reduction method. Comparing to the previous work, we encounter some new challenges because of competing potentials. By doing some delicate estimates for the competing potentials, we overcome the difficulties and find infinitely many positive solutions.
Application of machine learning procedures for mechanical system modelling: capabilities and caveats to prediction-accuracy
Authors: Groensfelder Thomas , Giebeler Fabian , Geupel Marco , Schneider David , Jaeger Rebecca 
Published: June 2020

AbstractThis article presents an investigation about prediction accuracy of multi-parametric models derived from numerical data. Three different mechanical test-cases are used for the generation of the numerical data. From this data, models are derived for the prediction of characteristic variation to arbitrary changes of the input parameters. Different modeling approaches are evaluated regarding their prediction accuracy. Polynomial matrix equations are compared to regression models and neural network models provided by Machine-Learning toolboxes. Similarities and differences of the models are worked out. An exponential matrix-equation-model is proposed to increase accuracy for certain applications. Influences and their causes to the prediction accuracy for the model predictions are evaluated. From this minimum requirements for deriving valuable models are defined. Leading to a comparison of the modelling approaches in relation to physical plausibility and model efficiency. Where efficiency is related to the effort for data creation and training-procedure. For one of the sample cases, a prediction-model is applied to demonstrate the model application and capabilities. The model equation is used to calculate the value of a penalty function in a multi-input/multi-output optimization task. As outcome of the optimization, four natural frequencies are fitted to measured values by updating material parameters. For all other cases sensitivity-studies including verification to numerical results are conducted.
Child passenger safety education in the emergency department: teen driving, car seats, booster seats, and more
Authors: Smola Cassi , Sorrentino Annalise , Shah Nipam , Nichols Michele , Monroe Kathy 
Published: June 2020

Abstract
Background
The leading cause of death in children less than 19 years old is motor vehicle crashes (MVC). Non-use or improper use of motor vehicle car seats significantly adds to the morbidity and mortality. Emergency department (ED) encounters provide an opportunity for caregiver education. Our objective was to determine the effect of an educational intervention on knowledge and counseling behaviors of pediatric ED nurses regarding child passenger safety (CPS).

Methods
A pre/post educational intervention study was conducted with nursing staff in an urban ED. Responses to CPS related knowledge and counseling behaviors were collected using surveys administered before and after the intervention. The ED nurse education intervention was a one-hour lecture based on the American Academy of Pediatrics (AAP) CPS guidelines and Alabama state law regarding ages for each car seat type and teen driving risky behaviors. Individual data from pre and post surveys were matched, and nominal variables in pre-post matched pairs were analyzed using McNemar’s test. To compare categorical variables within pre or post test data, we used the Chi-square test.

Results
Pretests were administered to 83/110 ED nurses; 64 nurses received the educational intervention and posttest. On the pretests, nurses reported “never” or “occasionally” counseling about CPS for the following: 56% car seats, 62% booster seat, 56% teen driving, 32% seat belts. When comparing the pretest CPS knowledge between nurses working 0-1 year vs. ≥ 2 years there was no statistically significant difference. Two CPS knowledge questions did not show significance due to a high correct baseline knowledge rate (> 98%), including baseline knowledge of MVC being the leading cause of death. Of the remaining 7 knowledge questions, 5 questions showed statistically significant improvement in knowledge: age when children can sit in front seat, state GDL law details, seat belt state law for back seat riders, age for booster seat, and rear facing car seat age. All four counseling behavior questions showed increases in intent to counsel families; however, only intent to counsel regarding teen driving reached statistical significance.

Conclusions
Educational efforts improved pediatric ED nursing knowledge regarding CPS. Intent to counsel was also improved following the education.
Expansion of a multi-pronged safe sleep quality improvement initiative to three children’s hospital campuses
Authors: Leong Traci , Roome Kerryn , Miller Terri , Gorbatkin Olivia , Singleton Lori , Agarwal Maneesha , Lazarus Sarah Gard 
Published: June 2020

Abstract
Background
The American Academy of Pediatrics (AAP) recommends infants should be Alone, on their Back, and in a clear Crib to combat relatively stagnant rates of sudden unexpected infant death (SUID). These are referred to as the ABCs of safe sleep. Studies have shown these recommendations are not consistently followed in the hospital setting, but further investigation would determine how to improve the rate of adherence. The objective of this study was to evaluate the impact of an expanded safe sleep initiative at three Georgia free-standing children’s hospital campuses before and after a multipronged safe sleep initiative.

Methods
A quality improvement program with a pre/post analysis was performed using a convenience method of sampling. Infants < 12 months old in three inpatient pediatric campuses were analyzed pre- and post- interventions. The intervention included: 1) nursing education, 2) identification of nurse “safe sleep” champions, 3) crib cards, 4) crib audits, and 5) weekly reporting of data showing nursing unit ABC compliance via tracking boards. The goal was ABC compliance of ≥25% for the post-intervention period. A standardized crib audit tool evaluated sleep position/location, sleep environment, and ABC compliance (both safe position/location and environment). Chi square analysis, Fisher’s exact test, and logistic regression were used to compare safe sleep behaviors before and after the interventions.

Results
There were 204 cribs included pre-intervention and 274 cribs post-intervention. Overall, there was not a significant change in sleep position/location (78.4 to 76.6%, p = 0.64). There was a significant increase in the percent of infants sleeping in a safe sleep environment following the intervention (5.9 to 39.8%, p < 0.01). Overall ABC compliance, including both sleep position/location and environment, improved from 4.4% pre-intervention to 32.5% post-intervention (p < 0.01). There was no significant variability between the hospitals (p = 0.71, p = 1.00).

Conclusions
The AAP’s safe sleep recommendations are currently not upheld in children’s hospitals, but safer sleep was achieved across three children’s campuses in this study. Significant improvements were made in sleep environment and overall safe sleep compliance with this multi-pronged initiative.