bluuebunny/crossref_metadata_2025_split huggingface dataset:
Susceptibility to Plasmodium falciparum Malaria: Influence of Combined Polymorphisms of IgG3 Gm Allotypes and Fc Gamma Receptors IIA, IIIA, and IIIB
Authors: Fall Abdou Khadre Dit Jadir , Dechavanne Celia , Sabbagh Audrey , Guitard Evelyne , Milet Jacqueline , Garcia André , Dugoujon Jean-Michel , Courtin David , Migot-Nabias Florence 
Published: December 2020

The binding of immunoglobulin (Ig) to Fc gamma receptors (FcgR) at the immune cell surface is an important step to initiate immunological defense against malaria. However, polymorphisms in receptors and/or constant regions of the IgG heavy chains may modulate this binding. Here, we investigated whether polymorphisms located in FcgR and constant regions of the heavy chain of IgG are associated with susceptibility toP. falciparummalaria. For this purpose, a clinical and parasitological follow-up on malaria was conducted among 656 infants in southern Benin. G3m allotypes (from total IgG3) were determined by a serological method of hemagglutination inhibition. FcgRIIA 131R/H and FcgRIIIA 176F/V genotypes were determined using the TaqMan method and FcgRIIIB NA1/NA2 genotypes were assessed by polymerase chain reaction using allele-specific primers. Association analyses between the number of malaria infections during the follow-up and polymorphisms in IgG G3m allotypes and FcgR were studied independently by zero inflated binomial negative regression. The influence of combinations of G3m allotypes and FcgRIIA/FcgRIIIA/FcgRIIIB polymorphisms on the number ofP. falciparuminfections, and their potential interaction with environmental exposure to malaria was assessed by using the generalized multifactor dimensionality reduction (GMDR) method. Results showed that individual carriage of G3m24 single allotype and of G3m5,6,10,11,13,14,24 phenotype was independently associated with a high risk of malaria infection. A risk effect for G3m6 was observed only under high environmental exposure. FcgRIIIA 176VV single genotype and combined carriage of FcgRIIA 131RH/FcgRIIIA 176VV/FcgRIIIB NA1NA2, FcgRIIA 131HH/FcgRIIIA 176FF/FcgRIIIB NA1NA1, FcgRIIA 131HH/FcgRIIIA 176VV/FcgRIIIB NA2NA2 and FcgRIIA 131HH/FcgRIIIA 176VV/FcgRIIIB NA1NA2 genotypes were related to a high number of malaria infections. The risk was accentuated for FcgRIIIA 176VV when considering the influence of environmental exposure to malaria. Finally, the GMDR analysis including environmental exposure showed strengthened associations with a malaria risk when FcgRIIA/FcgRIIIA/FcgRIIIB genotypes were combined to G3m5,6,11,24 and G3m5,6,10,11,13,15,24 phenotypes or G3m10 and G3m13 single allotypes. Our results highlight the relevance of studying IgG heavy chain and FcgR polymorphisms, independently as well as in combination, in relation to the individual susceptibility toP. falciparuminfection. The intensity of individual exposure to mosquito bites was demonstrated to impact the relationships found.
Management of Asymptomatic Sporadic Nonfunctioning Pancreatic Neuroendocrine Neoplasms (ASPEN) ≤2 cm: Study Protocol for a Prospective Observational Study
Authors: Partelli Stefano , Ramage John K. , Massironi Sara , Zerbi Alessandro , Kim Hong Beom , Niccoli Patricia , Panzuto Francesco , Landoni Luca , Tomazic Ales , Ibrahim Toni , Kaltsas Gregory , Bertani Emilio , Sauvanet Alain , Segelov Eva , Caplin Martyn , Coppa Jorgelina , Armstrong Thomas , Weickert Martin O. , Butturini Giovanni , Staettner Stefan , Boesch Florian , Cives Mauro , Moulton Carol Anne , He Jin , Selberherr Andreas , Twito Orit , Castaldi Antonio , De Angelis Claudio Giovanni , Gaujoux Sebastien , Almeamar Hussein , Frilling Andrea , Vigia Emanuel , Wilson Colin , Muffatti Francesca , Srirajaskanthan Raj , Invernizzi Pietro , Lania Andrea , Kwon Wooil , Ewald Jacques , Rinzivillo Maria , Nessi Chiara , Smid Lojze M. , Gardini Andrea , Tsoli Marina , Picardi Edgardo E. , Hentic Olivia , Croagh Daniel , Toumpanakis Christos , Citterio Davide , Ramsey Emma , Mosterman Barbara , Regi Paolo , Gasteiger Silvia , Rossi Roberta E. , Smiroldo Valeria , Jang Jin-Young , Falconi Massimo 
Published: December 2020

Introduction: The optimal treatment for small, asymptomatic, nonfunctioning pancreatic neuroendocrine neoplasms (NF-PanNEN) is still controversial. European Neuroendocrine Tumor Society (ENETS) guidelines recommend a watchful strategy for asymptomatic NF-PanNEN &lt;2 cm of diameter. Several retrospective series demonstrated that a non-operative management is safe and feasible, but no prospective studies are available. Aim of the ASPEN study is to evaluate the optimal management of asymptomatic NF-PanNEN ≤2 cm comparing active surveillance and surgery.Methods: ASPEN is a prospective international observational multicentric cohort study supported by ENETS. The study is registered in ClinicalTrials.gov with the identification code NCT03084770. Based on the incidence of NF-PanNEN the number of expected patients to be enrolled in the ASPEN study is 1,000 during the study period (2017–2022). Primary endpoint is disease/progression-free survival, defined as the time from study enrolment to the first evidence of progression (active surveillance group) or recurrence of disease (surgery group) or death from disease. Inclusion criteria are: age &gt;18 years, the presence of asymptomatic sporadic NF-PanNEN ≤2 cm proven by a positive fine-needle aspiration (FNA) or by the presence of a measurable nodule on high-quality imaging techniques that is positive at 68Gallium DOTATOC-PET scan.Conclusion: The ASPEN study is designed to investigate if an active surveillance of asymptomatic NF-PanNEN ≤2 cm is safe as compared to surgical approach.
A New Insight on the Role of the Cerebellum for Executive Functions and Emotion Processing in Adults
Authors: Beuriat Pierre-Aurélien , Cohen-Zimerman Shira , Smith Gretchen N. L. , Krueger Frank , Gordon Barry , Grafman Jordan 
Published: December 2020

Objective: We investigated whether the cerebellum plays a critical or supportive role in in executive and emotion processes in adults. Many investigators now espouse the hypothesis that participants with cerebellar lesions experience executive functions and emotions (EE) disorders. But we hypothesized that these disorders would be milder if the damage is relatively limited to the cerebellum compared to damage involving the cerebellum plus additional cortical areas.Methods: We studied veterans with penetrating Traumatic Brain Injury (pTBI) participating in the Vietnam Head Injury Study (VHIS). We selected veterans with a cerebellar lesion (n = 24), a prefrontal cortex lesion (n = 20), along with healthy controls (HC) (n = 55). Tests of executive functions and emotions were analyzed as well as caregiver burden. We performed between-group null hypothesis significance testing, Bayesian hypothesis tests and correlational analyses.Results: Performance of participants with cerebellar lesions which extended to the cerebral cortex was similar to the HC on the Executive Function tests but they were significantly impaired on the Working Memory Index. No differences were found on the emotional processing tasks with one exception—the Facial Expression of Emotion-Test (FEEST). We then examined a sub-group of participants with large cerebellar lesions (&gt;15%) but minimal lesions in the cerebral cortex (&lt;15%). This sub-group of participants performed similarly to the HC on the Working Memory Index and on the FEEST.Conclusions: We suggest that the cerebellar cortex may not be critical for executive functions or processing emotional stimuli in adults as suggested. Instead, we find that the cerebellum has a supportive role characterized by its computing of the motor requirements when EE processing is required.
Intraoperative Assessment of the Resection Specimen Facilitates Achievement of Adequate Margins in Oral Carcinoma
Authors: Smits Roeland W. H. , van Lanschot Cornelia G. F. , Aaboubout Yassine , de Ridder Maria , Hegt Vincent Noordhoek , Barroso Elisa M. , Meeuwis Cees A. , Sewnaik Aniel , Hardillo Jose A. , Monserez Dominiek , Keereweer Stijn , Mast Hetty , Hove Ivo Ten , Bakker Schut Tom C. , Baatenburg de Jong Robert J. , Puppels Gerwin J. , Koljenović Senada 
Published: December 2020

BackgroundInadequate resection margins in oral cavity squamous cell carcinoma have an adverse effect on patient outcome. Intraoperative assessment provides immediate feedback enabling the surgeon to achieve adequate resection margins. The goal of this study was to evaluate the value of specimen-driven intraoperative assessment by comparing the margin status in the period before and the period after the introduction of specimen-driven assessment as a standard of care (period 2010–2012 vs period 2013–2017).MethodsA cohort of patients surgically treated for oral squamous cell carcinoma at the Erasmus MC Cancer Institute, Rotterdam, between 2010–2012 was studied retrospectively and compared to results of a prospectively collected cohort between 2013–2017. The frequency, type and results of intraoperative assessment of resection margins were analyzed.ResultsOne hundred seventy-four patients were included from 2010–2012, 241 patients were included from 2013–2017. An increase in the frequency of specimen-driven assessment was seen between the two periods, from 5% in 2010–2012 to 34% in 2013–2017. When performing specimen-driven assessment, 16% tumor-positive resection margins were found in 2013–2017, compared to 43% tumor-positive resection margins overall in 2010–2012. We found a significant reduction of inadequate resection margins for specimen-driven intraoperative assessment (p &lt; 0.001). Also, tumor recurrence significantly decreased, and disease-specific survival improved when performing specimen-driven intraoperative assessment.ConclusionsSpecimen-driven intraoperative assessment improves resection margins and consequently, the outcome of oral cancer patients. We advocate this method as standard of care.
A Proteomics Signature of Mild Hypospadias: A Pilot Study
Authors: Piñeyro-Ruiz Coriness , Serrano Horacio , Jorge Inmaculada , Miranda-Valentin Eric , Pérez-Brayfield Marcos R. , Camafeita Emilio , Mesa Raquel , Vázquez Jesús , Jorge Juan Carlos 
Published: December 2020

Background and Objective:Mild hypospadias is a birth congenital condition characterized by the relocation of the male urethral meatus from its typical anatomical position near the tip of the glans penis, to a lower ventral position up to the brim of the glans corona, which can also be accompanied by foreskin ventral deficiency. For the most part, a limited number of cases have known etiology. We have followed a high-throughput proteomics approach to study the proteome in mild hypospadias patients.Methods:Foreskin samples from patients with mild hypospadias were collected during urethroplasty, while control samples were collected during elective circumcision (n= 5/group). A high-throughput, quantitative proteomics approach based on multiplexed peptide stable isotope labeling (SIL) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis was used to ascertain protein abundance changes in hypospadias patients when compared to control samples.Results:A total of 4,815 proteins were quantitated (2,522 with at least two unique peptides). One hundred and thirty-three proteins from patients with mild hypospadias showed significant abundance changes with respect to control samples, where 38 proteins were increased, and 95 proteins were decreased. Unbiased functional biological analysis revealed that both mitochondrial energy production and apoptotic signaling pathways were enriched in mild hypospadias.Conclusions:This first comprehensive proteomics characterization of mild hypospadias shows molecular changes associated with essential cellular processes related to energy production and apoptosis. Further evaluation of the proteome may expand the search of novel candidates in the etiology of mild hypospadias and could also lead to the identification of biomarkers for this congenital urogenital condition.
Occupational Stress and Mental Health: A Comparison Between Frontline Medical Staff and Non-frontline Medical Staff During the 2019 Novel Coronavirus Disease Outbreak
Authors: Zhang Xie , Zhao Ke , Zhang Guohua , Feng Ruihua , Chen Jianjun , Xu Dongwu , Liu Xiaodong , Ngoubene-Atioky Arlette J. , Huang Hong , Liu Yanlong , Chen Li , Wang Wei 
Published: December 2020

Background: During an epidemic, both frontline and non-frontline medical staff endure stressful work circumstances that render their mental health a major public health concern. This study aims at investigating and comparing the prevalence and severity of mental health symptoms (i.e., anxiety, depression and insomnia) between frontline medical staff and non-frontline medical staff during the coronavirus disease 2019 (COVID-19) outbreak. It also seeks to evaluate the association of their mental health with occupational stress.Methods: A cross-sectional study was conducted in Wenzhou, China from 2020 February 16th to 2020 March 2th. A total of 524 medical staff responded to the Generalized Anxiety Disorder Scale, the Patient Health Questionnaire, the Insomnia Severity Index, the Occupational stress Questionnaire, and a demographic data form. Data were principally analyzed with logistic regression.Results: Of the 524 participants, 31.3% reported depression, 41.2% reported anxiety, and 39.3% reported insomnia. Compared with the citizens during the COVID-19 epidemic, medical staff experienced higher level of anxiety, depression and insomnia, especially the frontline medical staff. Furthermore, male, married medical staff with poorer physical health reported lower mental health. Frontline medical staff endorsed higher self-reported occupational stress, especially higher occupational hazards, than non-frontline medical staff. In addition, four indicators on occupational stress (working intensity, working time, working difficulty and working risk) were correlated positively with mental health symptoms. Regression analyses found a significant association between occupational stress and mental health symptoms in both frontline and non-frontline medical staff during COVID-19 outbreak.Conclusion: The results indicated that during the COVID-19 epidemic, medical staff experienced higher levels of anxiety, depression and insomnia than citizens, and their occupational stress had positive effects on their psychological distress. These findings emphasize the importance of occupational stress management interventions to decrease the risk of developing mental health problems among the medical staff during a biological disaster.
Association Analysis of Neuronal Nitric Oxide Synthase 1 Gene Polymorphism With Psychopathological Symptoms in Chronic Ketamine Users
Authors: Chen Jiansong , Zhang Minling , Zhou Chao , Ding Yi , Fan Ni , He Hongbo 
Published: December 2020

Objective: We previously found that chronic ketamine usages were associated with various psychotic and cognitive symptoms mimicking schizophrenia. The blockade of the NMDA receptor and subsequent nitric oxide synthase 1 (NOS1) dysfunction were found to be closely correlated with schizophrenia including NOS1 gene polymorphisms. We examined the allelic variants of the gene coding neuronal nitric oxide synthase 1 (NOS1) in chronic ketamine users in the Chinese population and analyzed the association between NOS1 gene polymorphism and psychopathological symptoms in chronic ketamine users. The association between the NOS1 polymorphism and ketamine use characteristics was also examined.Methods: One hundred ninety seven male chronic ketamine users and 82 controls were recruited. Four common SNPs of the NOS1 gene, rs6490121, rs41279104, rs3782206, and rs3782219, were examined by real-time PCR with the TaqMan® assay system. Psychopathological symptoms were assessed using the Positive and Negative Syndrome Scale (PANSS), Beck Depression Inventory (BDI), and the Beck Anxiety Inventory (BAI).Results: The genotype distribution of rs6490121 and rs41279104 in chronic ketamine users was significantly different from that in the control (p = 0.0001 and p = 0.002). The G allele frequency of rs6490121 in ketamine users was higher than that in the control (p = 2.23 * 10−6, OR = 3.07, 95% CI = 1.93–4.90). The T allele frequency of rs41279104 in chronic ketamine users was higher than that in the control (p = 0.01, OR = 1.76, 95% CI = 1.14–2.72). The BAI score was significantly different among the three genotypic groups of rs6490121 (F = 6.21, p = 0.002) in ketamine users; subjects of genotype AG and GG had a lower score than subjects of genotype AA. The score of the negative symptom subscale of PANSS was significantly different among the three genotypic groups of rs41279104 (F = 5.39, p = 0.005); in ketamine users, subjects of genotype CT and TT had a higher score than subjects of genotype CC. There was no difference in drug use characteristics in different genotypes of the four NOS1 gene polymorphisms tested in ketamine users (p &gt; 0.05).
FORMATION OF ARCHITECTURAL ENVIRONMENT OF A HISTORICAL CENTER CITIES OF ROSTOV-ON-DON
Authors: Ivanenko O , Belova A. 
Published: December 2020

In the study the features of the formation of historical and state-of-the-art architecture of Rostov-on-Don were observed. The features of architecture in the context of the historical and architectural development of the city. Tendencies of development of the environmental approach in buildings using, as examples, the historical monuments of cultural heritage and new present-day structures based on field-scale and scientific research.
Relevance of the study is devoted to problem of development of architectural environment of historical center cities, including the Rostov-on-Don, to save its completeness and authenticity.
The goal of study is to analyze a nowadays architectural environment of a center city in the Rostov-on-Don, using the some of the samples, as well as to analyze the effect of some state-f-the-art construction objects on the city architecture.
The task of the study is to determine the effect of present-day buildings on architectural-artistic appearance of architectural environment of a center city in the Rostov-on-Don.
Rostov-on-Don city is unique and from the architectural point of view. Its appearance was formed during a 300 years.
In the study historical environment of Rostov-on-Don was observed basing on some of the examples of present-day and historical construction of buildings, their impact on the perception of the integrity and authenticity of the architectural environment. Examples of successful and negative experiences were given. The analysis of the perception of the historical appearance of the Rostov-on-Don city was realized.
Educación Sexual Integral: reflexiones sobre experiencias institucionales en el nivel secundario
Authors: Vázquez María Soledad 
Published: December 2020

En este artículo pongo en diálogo diferentes experiencias de Educación Sexual que tuvieron lugar en el nivel secundario de una institución de gestión privada con otras desarrolladas en un Bachillerato Popular. En la primera, se escolarizan adolescentes de entre 12 y 18 años que, en su mayoría, son de sectores socioeconómicos medios y medios-altos; en la segunda, jóvenes y adultos que atraviesan profundas vulnerabilidades materiales y simbólicas. Ambas escuelas son laicas y se ubican en diferentes municipios de la provincia de Buenos Aires.
Se trata de experiencias sucedidas en los últimos cuatro años, a las que accedo desarrollando dos roles diferentes: en la escuela de gestión privada, como docente orientadora con tareas de Educación Sexual Integral a cargo; en el Bachillerato Popular, como investigadora, en el marco del trabajo de campo de mi tesis doctoral.
Mi tesis está centrada en las escolaridades y en los vínculos que ocho jóvenes con experiencias de maternidad y de sectores populares sostienen con sus propias madres. Su metodología es el enfoque biográfico. Actualmente, estoy abocada a su escritura.
En ambas instituciones, ya sea como docente orientadora o como investigadora, he visualizado que varios estudiantes y sus familias rechazan la Educación Sexual Integral, sobre todo el tratamiento de dos de sus dimensiones claves: el respeto por la diversidad y el ejercicio de los derechos. Sin embargo, los conflictos asociados a estos rechazos son abordados institucionalmente de diferentes modos. Como desarrollo en este artículo, en la escuela de gestión privada la conducción directiva procura evitar cualquier tipo de controversia silenciando las temáticas rechazadas. En el Bachillerato Popular, en cambio, se consideran los conflictos como parte de las condiciones de abordaje de la sexualidad en la escuela y, desde allí, se habilitan espacios pedagógicos para su despliegue.
PRODUCTION, PURIFICATION AND INHIBITION OF ALGINATE LY-ASE FROM LOCAL ISOLATE OF PSEUDOMONAS AERUGINOSA NA11
Authors: Younis R. W. 
Published: December 2020

The point of this study was for determine of the optimum conditions and purification of alginate lyase from local isolate of Pseudomonas aeruginosa NA11, and inhibition of enzyme by various plant extracts. Forty local isolate s of pathogenic Pseudomonas aeruginosa were screened for their ability to produce alginate lyase. Local isolate of P.aeruginosa NA11 showed the maximum efficiency for produce of alginate lyase with high specific activity (14.4 U/mg). Several factors that influence on alginate lyase production from local isolate of P.aeruginosa NA11 were studied, these factors included the type of media, carbon source, nitrogen source, the incubation temperature, pH, and the incubation period. The highest yield of alginate lyase was obtained with the A medium supplemented with 0.5 % of glucose and sodium nitrate at pH 7.5 after 24 hr. incubation at 37 °C. Two chromatographic techniques were used for purification of alginate lyase after precipitation by ammonium sulphate with saturated ratio (0-70 %), including, ion exchange chromatography by DEAE-cellulose and gel filtration by Sephadex G-100. The two steps gave the specific activity of 155.8 U/mg protein, the purification fold was 4.15 and enzymatic yield was 64 %. The molecular weight of partial purified alginate lyase was 57 KDa. The results of antioxidant activity tests for different plants extracts utilizing DPPH radical scavenging activity were showed that the saad extract has high antioxidant activity (71 %) more than other plants extracts. While the results for inhibition experiment of alginate lyase were demonstrated that saad extract was the best inhibitor with inhibition ratio of 86 %.
The Third Data Release of the KODIAQ Survey
Authors: O’Meara John M. , Lehner Nicolas , Howk J. Christopher , Prochaska J. Xavier 
Published: December 2020

Abstract
We present and make publicly available the third data release (DR3) of the Keck Observatory Database of Ionized Absorption toward Quasars (KODIAQ) survey. KODIAQ DR3 consists of a fully reduced sample of 727 quasars at 0.1 < z
em < 6.4 observed with the Echellette Sepctrograph and Imager at moderate resolution (4000 ≤ R ≤ 10,000). DR3 contains 872 spectra available in flux calibrated form, representing a sum total exposure time of ∼2.8 megaseconds. These coadded spectra arise from a total of 2753 individual exposures of quasars taken from the Keck Observatory Archive (KOA) in raw form and uniformly processed using a data reduction package made available through the XIDL distribution. DR3 is publicly available to the community, housed as a higher level science product at the KOA and in the igmspec database.