bluuebunny/crossref_metadata_2025_split huggingface dataset:
Duplex real-time PCR assay for the simultaneous detection ofAchromobacter xylosoxidansandAchromobacterspp
Authors: Price Erin P. , Soler Arango Valentina , Kidd Timothy J. , Fraser Tamieka A. , Nguyen Thuy-Khanh , Bell Scott C. , Sarovich Derek S. 
Published: February 2020

AbstractSeveral members of the Gram-negative environmental bacterial genus,Achromobacter, are associated with serious infections in immunocompromised individuals, of whichAchromobacter xylosoxidansis the most common. Despite their pathogenic potential, comparatively little is understood about these intrinsically drug-resistant bacteria and their role in disease, leading to suboptimal diagnosis and management ofAchromobacterinfections. Here, we performed comparative genomics of 158Achromobacterspp. genomes to robustly identify species boundaries, to reassign several incorrectly speciated taxa, and to identify genetic sequences specific for theAchromobactergenus and forA. xylosoxidans. Next, we developed a Black Hole Quencher probe-based duplex real-time PCR assay, Ac-Ax, for the rapid and simultaneous detection ofAchromobacterspp. andA. xylosoxidansfrom both purified colonies and polymicrobial clinical specimens. Ac-Ax was tested on 119 isolates identified asAchromobacterspp. using phenotypic or genotypic methods. In comparison to these routine diagnostic methods, the duplex assay showed superior identification ofAchromobacterspp. andA. xylosoxidans, with fiveAchromobacterisolates failing to amplify with Ac-Ax confirmed to be different genera according to 16S rRNA gene sequencing. Ac-Ax quantified bothAchromobacterspp. andA. xylosoxidansdown to ∼110 genome equivalents, and detected down to ∼12 and ∼1 genome equivalent/s, respectively.In silicoanalysis, and laboratory testing of 34 non-Achromobacterisolates and 38 adult CF sputa, confirmed duplex assay specificity and sensitivity. We demonstrate that the Ac-Ax duplex assay provides a robust, sensitive, and cost-effective method for the simultaneous detection of allAchromobacterspp. andA. xylosoxidans, and will facilitate the rapid and accurate diagnosis of this important group of pathogens.
Association Study of Over 200,000 Subjects Detects Novel Rare Variants, Functional Elements, and Polygenic Architecture of Prostate Cancer Susceptibility
Authors: Emami Nima C. , Cavazos Taylor B. , Rashkin Sara R. , Cario Clinton L. , Graff Rebecca E. , Tai Caroline G. , Mefford Joel A. , Kachuri Linda , Wan Eunice , Wong Simon , Aaronson David S. , Presti Joseph , Habel Laurel A. , Shan Jun , Ranatunga Dilrini K. , Chao Chun R. , Ghai Nirupa R. , Jorgenson Eric , Sakoda Lori C. , Kvale Mark N. , Kwok Pui-Yan , Schaefer Catherine , Risch Neil , Hoffmann Thomas J. , Eeden Stephen K. Van Den , Witte John S. 
Published: February 2020

ABSTRACTThe potential association between rare germline genetic variants and prostate cancer (PrCa) susceptibility has been understudied due to challenges with assessing rare variation. Furthermore, although common risk variants for PrCa have shown limited individual effect sizes, their cumulative effect may be of similar magnitude as high penetrance mutations. To identify rare variants associated with PrCa susceptibility, and better characterize the mechanisms and cumulative disease risk associated with common risk variants, we analyzed large population-based cohorts, custom genotyping microarrays, and imputation reference panels in an integrative study of PrCa genetic etiology. In particular, 11,649 men (6,196 PrCa cases, 5,453 controls) of European ancestry from the Kaiser Permanente Research Program on Genes, Environment and Health, ProHealth Study, and California Men’s Health Study were genotyped and meta-analyzed with 196,269 European-ancestry male subjects (7,917 PrCa cases, 188,352 controls) from the UK Biobank. Six novel loci were genome-wide significant in our meta-analysis, including two rare variants (minor allele frequency < 0.01, at 3p21.31 and 8p12). Gene-based rare variant tests implicated a previously discovered PrCa gene (HOXB13) as well as a novel candidate (ILDR1) highly expressed in prostate tissue. Haplotypic patterns of long-range linkage disequilibrium were observed for rare genetic variants atHOXB13and other loci, reflecting their evolutionary history. Furthermore, a polygenic risk score (PRS) of 187 known, largely common PrCa variants was strongly associated with risk in non-Hispanic whites (90thvs. 10thdecile OR = 7.66,P= 1.80*10-239). Many of the 187 variants exhibited functional signatures of gene expression regulation or transcription factor binding, including a six-fold difference in log-probability of Androgen Receptor binding at the variant rs2680708 (17q22). Our finding of two novel rare variants associated with PrCa should motivate further consideration of the role of low frequency polymorphisms in PrCa, while the considerable effect of PrCa PRS profiles should prompt discussion of their role in clinical practice.
The Pebble/Rho1/Anillin pathway controls polyploidization and axonal wrapping activity in the glial cells of theDrosophilaeye
Authors: Tavares Lígia , Grácio Patrícia , Ramos Raquel , Traquete Rui , Relvas João B. , Pereira Paulo S. 
Published: February 2020

AbstractDuring development glial cell are crucially important for the establishment of neuronal networks. Proliferation and migration of glial cells can be modulated by neurons, and in turn glial cells can differentiate to assume key roles such as axonal wrapping and targeting. To explore the roles of actin cytoskeletal rearrangements in glial cells, we studied the function of Rho1 inDrosophiladeveloping visual system. We show that the Pebble (RhoGEF)/Rho1/Anillin pathway is required for glia proliferation and to prevent the formation of large polyploid perineurial glial cells, which can still migrate into the eye disc if generated. Surprisingly, this Rho1 pathway is not necessary to establish the total glial membrane area or for the differentiation of the polyploid perineurial cells. The resulting polyploid wrapping glial cells are able to initiate wrapping of axons in the basal eye disc, however the arrangement and density of glia nuclei and membrane processes in the optic stalk are altered and the ensheathing of the photoreceptor axonal fascicles is reduced.
Experimental and computational studies on heterocyclic organic compounds as corrosion inhibitors for copper immersed in O/W emulsion medium
Authors: Xiong Sang , Si Jia , Sun Jianlin , Wu Hao , Dong Hongchen , Zhang Chao 
Published: February 2020

PurposeCorrosion inhibitors for copper immersed in emulsion were investigated by experiments and theoretical calculations, and this study aims to propose a new inhibition mechanism of the inhibitors having protective effects for copper corrosion.Design/methodology/approachAdsorption behavior of penta-heterocycles (thiophene, 1,2,5-oxadiazole, furan, 2 H-1,2,3-triazole, pyrrole and 1,2,5-thiadiazole) as corrosion inhibitors for copper immersed in oil-in-water (O/W) emulsions was investigated by weight loss, electrochemical tests, morphological characterization and theoretical calculations.FindingsThe orders of inhibition effect are furan < pyrrole < thiophene < 1,2,5-oxadiazole < 2H-1,2,3-triazole < 1,2,5-thiadiazole, and 1,2,5-thiadiazole at 0.5 mM has the best inhibition effect for copper immersed in emulsion. The results of scanning probe microscope, scanning electron microscope and electrochemical test show that a protective barrier can be formed on the surface of copper substrate with six corrosion inhibitors, thus effectively inhibiting the corrosion of copper mainly through chemisorption and following Langmuir’s adsorption isotherm.Originality/valueQuantum chemical and molecular dynamic simulations demonstrate that all these compounds attached to Cu matrix with a flat-adsorption mode to prevent the emulsion corrode copper. Adsorbed inhibitors act as a barrier at Cu matrix to block corrosion and improve hydrophobicity.
DEA window analysis and Malmquist index to assess efficiency and productivity in the Spanish electricity sector
Authors: Sánchez-Ortiz Jaime , Garcia-Valderrama Teresa , Rodríguez-Cornejo Vanesa , Cabrera-Monroy Francisca 
Published: February 2020

Purpose
The purpose of this paper is to demonstrate that overcapacity and tariff deficit (external constraints) negatively affect the efficiency of distribution firms in the Spanish electricity sector. To do this, the paper is based on the theory of constraints and theory of economic regulation.

Design/methodology/approach
Data envelopment analysis (DEA) window methodology is carried out on the constant scales (I-C) with a sample consisting of five main distribution firms during the period from 2006 to 2015. In turn, an analysis of the Malmquist index is carried out to assess whether it has had a displacement with respect to the efficiency frontier.

Findings
The results show that the overcapacity and the tariff deficit negatively affect the efficiency of the distribution firms of the Spanish electricity sector. In addition, there is an existence of external constraints that affect the activities of regulated organisations and the importance of adequate legislation in regulated sectors.

Originality/value
This study defines a model that shows how the efficiency problems associated with electricity distribution companies such as productive overcapacity or tariff deficit can be measured based on the theory of constraints and theory of economic regulation.
Complexity drivers in engineering design
Authors: Benabdellah Abla Chaouni , Benghabrit Asmaa , Bouhaddou Imane 
Published: February 2020

PurposeIn the era of industry 4.0, managing the design is a challenging mission. Within a dynamic environment, several disciplines have adopted the complex adaptive system (CAS) perspective. Therefore, this paper aims to explore how we may deepen our understanding of the design process as a CAS. In this respect, the key complexity drivers of the design process are discussed and an organizational decomposition for the simulation of the design process as CAS is conducted.Design/methodology/approachThe proposed methodology comprises three steps. First, the complexity drivers of the design process are presented and are matched with those of CAS. Second, an analysis of over 111 selected papers is presented to choose the appropriate model for the design process from the CAS theory. Third, the paper provides methodological guidelines to develop an organizational decision support system that supports the complexity of the design process.FindingsAn analysis of the key drivers of design process complexity shows the need to adopt the CAS theory. In addition to that, a comparative analysis between all the organizational methodologies developed in the literature leads the authors to conclude that agent-oriented Software Process for engineering complex System is the appropriate methodology for simulating the design process. In this respect, a system requirements phase of the decision support system is conducted.Originality/valueThe originality of this paper lies in the fact of analysing the complexity of the design process as a CAS. In doing so, all the richness of the CAS theory can be used to meet the challenges of those already existing in the theory of the design.
Why organizational loyalty programs cannot prevent switching
Authors: Mattison Thompson Frauke , Tuzovic Sven 
Published: February 2020

Purpose
The purpose of this study is to investigate the extent to which loyalty programs can prevent switching, and how individual level cultural values impact this. Loyalty programs are designed to create switching costs, which reduce customers’ desire to leave. However, in practice, these programs are often misapplied; that is, most companies inadvertently treat all customers as equal. While ample research has examined the role of loyalty reward programs in facilitating customer loyalty, little is known about the extent to which individual-level cultural values moderate customer loyalty measures of trust and affective commitment and how this impacts the effectiveness of loyalty programs; that is, consumers’ intentions to “stick” with the program or to switch.

Design/methodology/approach
This study uses a quasi-experiment combined with an extensive survey to collect the data.

Findings
Based on data collected from one industrial country and four emerging countries, the results show that loyalty programs do not universally prevent switching behavior. Instead, this study finds that individual-level uncertainty avoidance and collectivist values significantly moderate the effects.

Originality/value
This study helps advance the understanding of how international retailers can increase their loyalty program effectiveness and reduce customer switching to competitors.
Impact of surface acting and deep acting techniques on teachers’ organizational commitment
Authors: Ogunsola Kazeem Olanrewaju , Fontaine Rodrique Ancelot Harvey , Jan Muhammad Tahir 
Published: February 2020

PurposeThis paper aims to examine the relationship between surface acting (SA), deep acting (DA) and organizational commitment (OC).Design/methodology/approachGuided by affective events theory, the study adapted emotional labour scale and three components model to profile 373 teachers from 30 schools around Peninsular Malaysia. A list-based simple random sampling technique was used to select respondents. Structural equation modeling (SEM) was used to test hypotheses, and the proposed model was assessed through renowned fit indices.FindingsOC was hypothesized as a second-order construct. SEM result indicates that both SA and DA have significant negative relationship with OC. Fit indices of the hypothesized model showed χ²/dfratio (560.069/265) = 2.113, RMSEA (0.055), and CFI (0.936). This result provides empirical support for the data collected.Research limitations/implicationsThe study provides new insight on the ongoing debate about SA and DA. Therefore, it advances body of research in this regard. The implication for HR managers is that strategic polices can be institutionalized to buffer the consequences of SA and DA. This is due to the fact that SA and DA may not be abolished for service employees like teachers. The practical implication for teachers is the understanding that emotional regulation process is inevitable because teaching is profoundly an emotional activity job. Besides being a cross-sectional study, the sampled population may have limited the study’s outcomes.Originality/valueGiven existing inconsistent results on the consequences of SA and DA, this study shows that not only SA can lead to negative after-effects, DA can also cause the same. Future study can explore spiritual intelligence to examine how best SA and DA can be performed at reduced consequences on OC.
From CSR to CSI
Authors: Vollero Agostino , Palazzo Maria , Siano Alfonso , Foroudi Pantea 
Published: February 2020

Purpose
The purpose of this paper is to analyses consumers’ hostile responses and “creative” re-interpretation of a proactive corporate social responsibility (CSR) brand communication campaign on social media by a leading Italian company in the energy industry that came to be perceived as a reactive corporate social irresponsibility performance.

Design/methodology/approach
Taking Palazzo and Basu’s framework of scapes (2007) as a starting point, the paper explores the intersection between branding and CSR studies. After consideration of the lack of empirical studies on this subject, a content analysis of tweets generated from the campaign “Guerrieri” of Enel is performed.

Findings
Findings show the dialogic bottom-up approach results are ineffective because of the hijacking of original intent of the company in implementing its CSR communication initiatives. That is to say that corporate brand (CB) strategies can be easily re-interpreted in social media-scape from an opposing perspective, raising the risk of digital hijacking and boycotting initiatives.

Practical implications
From a practical standpoint, the study informs managers so that they can evaluate complex problems implicated in the creation of CSR activities aimed at engaging consumers and virtual communities. Besides, the paper would like to aid managers when they face discontent or activism, suggesting they should turn the attention of their stakeholders through a re-evaluation of relevant CSR activities, potentially leveraging on a loyal public, which has completely interiorised CB values and can act as brand ambassadors.

Originality/value
The paper is one of the first attempts to study the nexus between CSR and CB in digitally empowering contexts, clarifying the crucial role of social media-scape.
The impact of chorionicity on pregnancy outcome and neurodevelopment at 2 years old among twins born preterm: the EPIPAGE‐2 cohort study
Authors: Tosello B , Garbi A , Blanc J , Lorthe E , Foix‐L’Hélias L , D’Ercole C , Winer N , Subtil D , Goffinet F , Kayem G , Resseguier N , Gire C ,   for the EPIPAGE‐2 Obstetric Writing Group
Published: February 2020

ObjectiveTo compare the short‐ and mid‐term outcomes of preterm twins by chorionicity of pregnancy.DesignProspective nationwide population‐based EPIPAGE‐2 cohort study.Setting546 maternity units in France, between March and December 2011.PopulationA total of 1700 twin neonates born between 24 and 34 weeks of gestation.MethodsThe association of chorionicity with outcomes was analysed using multivariate regression models.Main outcome measuresFirst, survival at 2‐year corrected age with or without neurosensory impairment, and second, perinatal, short‐, and mid‐term outcomes (survival at discharge, survival at discharge without severe morbidity) were described and compared by chorionicity.ResultsIn the EPIPAGE 2 cohort, 1700 preterm births were included (850 twin pregnancies). In all, 1220 (71.8%) were from dichorionic (DC) pregnancies and 480 from monochorionic (MC) pregnancies. MC pregnancies had three times more medical terminations than DC pregnancies (1.67 versus 0.51%, P < 0.001), whereas there were three times more stillbirths in MC than in DC pregnancies (10.09 versus 3.78%, P < 0.001). Both twins were alive at birth in 86.6% of DC pregnancies compared with 80.0% among MC pregnancies (P = 0.008). No significant difference according to chorionicity was found regarding neonatal deaths and morbidities. Likewise, for children born earlier than 32 weeks, the 2‐year follow‐up neurodevelopmental results were not significantly different between DC and MC twins.ConclusionsThis study confirms that MC pregnancies have a higher risk of adverse outcomes. However, the outcomes among preterm twins admitted to neonatal intensive care units are similar irrespective of chorionicity.Tweetable abstractMonochorionicity is associated with adverse perinatal outcomes, but outcomes for preterm twins are comparable irrespective of their chorionicity.
Cardio‐metabolic risk factors among young infertile women: a systematic review and meta‐analysis
Authors: Mulder CL , Lassi ZS , Grieger JA , Ali A , Jankovic‐Karasoulos T , Roberts CT , Andraweera PH 
Published: February 2020

BackgroundThere is currently no concise systematic review or meta‐analysis addressing cardio‐metabolic risk factors in women experiencing infertility.ObjectivesTo determine whether infertile women have higher levels of cardiovascular risk factors compared with fertile women.Search strategyWe performed a systematic literature search using PubMed, Embase and CINAHL, Scopus and additional manual and bibliographic searches for relevant articles (end search date 6 November 2019).Selection criteriaWe selected studies that compared cardio‐metabolic risk factors in fertile and infertile women of reproductive age.Data collection and analysisAt least two authors independently screened potentially eligible studies.Main resultsThere was an increased presence of several cardio‐metabolic risk factors in infertile women compared with fertile women. Infertile women had statistically significant higher body mass index (BMI), increased total cholesterol (TC), low‐density lipoprotein cholesterol (LDL‐C) and triglycerides (TG) compared with fertile women. Fasting glucose, fasting insulin, homeostatic model assessment of insulin resistance (HOMA‐IR) and mean arterial pressure were not found to be different between fertile and infertile women. A subgroup analysis revealed that TC, fasting glucose and fasting insulin were increased, and high‐density lipoprotein was decreased only in women with polycystic ovarian syndrome compared with fertile women, whereas BMI, TG and LDL‐C were statistically significantly increased in women with any indication of infertility compared with fertile women.ConclusionsInfertile women have a higher level of cardio‐metabolic risk factors compared with fertile women. This finding has clinical implications for infertile women in general, and those attempting to conceive through medically assisted reproduction.Tweetable abstractInfertile women appear to have a higher level of cardio‐metabolic risk factors compared with fertile women.
Operationalizing ecological connectivity in spatial conservation planning with Marxan Connect
Authors: Daigle Rémi M. , Metaxas Anna , Balbar Arieanna C. , McGowan Jennifer , Treml Eric A. , Kuempel Caitlin D. , Possingham Hugh P. , Beger Maria 
Published: February 2020

AbstractGlobally, protected areas are being established to protect biodiversity and to promote ecosystem resilience. The typical spatial conservation planning process leading to the creation of these protected areas focuses on representation and replication of ecological features, often using decision support tools such as Marxan. Yet, despite the important role ecological connectivity has in metapopulation persistence and resilience, Marxan currently requires manual input or specialized scripts to explicitly consider connectivity.‘Marxan Connect’ is a new open source, open access Graphical User Interface (GUI) tool designed to assist conservation planners with the appropriate use of data on ecological connectivity in protected area network planning.Marxan Connect can facilitate the use of estimates of demographic connectivity (e.g. derived from animal tracking data, dispersal models, or genetic tools) or structural landscape connectivity (e.g. isolation by resistance). This is accomplished by calculating metapopulation‐relevant connectivity metrics (e.g. eigenvector centrality) and treating those as conservation features or by including the connectivity data as a spatial dependency amongst sites in the prioritization process.Marxan Connect allows a wide group of users to incorporate directional ecological connectivity into conservation planning with Marxan. The solutions provided by Marxan Connect, combined with ecologically relevant post‐hoc testing, are more likely to support persistent and resilient metapopulations (e.g. fish stocks) and provide better protection for biodiversity.