bluuebunny/crossref_metadata_2025_split huggingface dataset:
Loss of the RNA helicaseDhx15impairs endothelial energy metabolism, lymphatic drainage and tumor metastasis in mice
Authors: Ribera Jordi , Portolés Irene , Córdoba Bernat , Rodríguez-Vita Juan , Casals Gregori , de la Presa Bernardino González , Graupera Mariona , Soria Guadalupe , Tudela Raúl , Esteve-Codina Anna , Espadas Guadalupe , Sabidó Eduard , Jiménez Wladimiro , Sessa William C. , Morales-Ruiz Manuel 
Published: November 2020

ABSTRACTDHX15 is an ATP-dependent RNA helicase involved in pre-mRNA splicing and a downstream substrate for Akt1, which plays a significant role in vascular biology. The aim of this study was to explore the regulatory function of DHX15 over the vasculature and endothelial cell biology.Results:DHX15-/-was lethal in mouse and zebrafish embryos. DHX15-/-zebrafish also showed an undeveloped parachordal line, which leads to the formation of lymphatic structures. DHX15+/-mice triggered lower vascular network density and impaired lymphatic function postnatally. Transcriptome and proteome analysis of DHX15 silenced LEC revealed alterations in the glycolysis and gluconeogenesis pathways. The validation of these results demonstrated an uncoupling of the glycolysis with the oxidation of pyruvate in the mitochondria and a lower activity of the Complex I, resulting in lower cellular ATP production. Noteworthy, DHX15+/-mice partially inhibited primary tumor growth and reduced lung metastasis after injection of LLC1 tumor cells.
Heritability of cortical morphology reflects a sensory-fugal plasticity gradient
Authors: Vainik Uku , Paquola Casey , Wang Xindi , Zheng Yingqiu , Bernhardt Boris , Misic Bratislav , Dagher Alain 
Published: November 2020

AbstractHuman brain plastically adapts to environmental demands. Here, we propose that naturally occuring plasticity in certain brain areas should be reflected by higher environmental influence and therefore lower heritability of the structure of those brain areas. Mesulam’s (1998) seminal overview proposed a hierarchy of plasticity, where higher-order multimodal areas should be more plastic than lower-order sensory areas. Using microstructural and functional gradients as proxies for Mesulam’s hierarchy, we seek to test whether these gradients predict heritability of brain structure. We test this model simultaneously across multiple measures of cortical structure and microstructure derived from structural magnet resonance imaging. We also account for multiple other explanations of heritability differences, such as signal-to-noise ratio and spatial autocorrelation. We estimated heritability of brain areas using 984 participants from the Human Connectome Project. Multi-level modelling of heritability differences demonstrated that heritability is explained by both signal quality, as well as by the primary microstructural gradient. Namely, sensory areas had higher heritability and limbic/heteromodal areas had lower heritability. Given the increasing availability of genetically informed imaging data, heritability could be a quick method assess brain plasticity.Highlights (up to 85 chars)Cortical areas vary in heritability. This is seen across structural measures.Heritability differences could be explained by plasticity, topography, or noise.We build a comprehensive model testing many explanations across 5 measures.Heritability is explained by noise and 1ststructural gradient reflecting plasticity.Heritability could be a method to study brain plasticity.
Calcium depletion challenges endoplasmic reticulum proteostasis by destabilising BiP-substrate complexes
Authors: Preissler Steffen , Rato Claudia , Yan Yahui , Perera Luke A. , Czako Aron , Ron David 
Published: November 2020

AbstractThe metazoan endoplasmic reticulum (ER) serves both as a hub for maturation of secreted proteins and as an intracellular calcium storage compartment, facilitating calcium release-dependent cellular processes. ER calcium depletion robustly activates the unfolded protein response (UPR). However, it is unclear how fluctuations in ER calcium impact organellar proteostasis. Here we report that calcium selectively affects the dynamics of the abundant metazoan ER Hsp70 chaperone BiP, by enhancing its affinity for ADP. In the calcium replete ER, ADP rebinding to post-ATP hydrolysis BiP-substrate complexes competes with ATP binding during both spontaneous and co-chaperone-assisted nucleotide exchange, favouring substrate retention. Conversely, in the calcium depleted ER, relative acceleration of ADP-to-ATP exchange favours substrate release. These findings explain the rapid dissociation of certain substrates from BiP observed in the calcium-depleted ER and suggest a mechanism for tuning ER quality control and coupling UPR activity to signals that mobilise ER calcium in secretory cells.
Modelling the active SARS-CoV-2 helicase complex as a basis for structure-based inhibitor design
Authors: Berta Dénes , Badaoui Magd , Buigues Pedro J. , Martino Sam Alexander , Pisliakov Andrei V. , Elghobashi-Meinhardt Nadia , Wells Geoff , Harris Sarah A. , Frezza Elisa , Rosta Edina 
Published: November 2020

ABSTRACTHaving claimed over 1 million lives worldwide to date, the ongoing COVID-19 pandemic has created one of the biggest challenges to develop an effective drug to treat infected patients. Among all the proteins expressed by the virus, RNA helicase is a fundamental protein for viral replication, and it is highly conserved among the coronaviridae family. To date, there is no high-resolution structure of helicase bound with ATP and RNA. We present here structural insights and molecular dynamics (MD) simulation results of the SARS-CoV-2 RNA helicase both in its apo form and in complex with its natural substrates. Our structural information of the catalytically competent helicase complex provides valuable insights for the mechanism and function of this enzyme at the atomic level, a key to develop specific inhibitors for this potential COVID-19 drug target.
Structure of nonstructural protein 1 from SARS-CoV-2
Authors: Clark Lauren K. , Green Todd J. , Petit Chad M. 
Published: November 2020

ABSTRACTThe periodic emergence of novel coronaviruses (CoVs) represents an ongoing public health concern with significant health and financial burden worldwide. The most recent occurrence originated in the city of Wuhan, China where a novel coronavirus (SARS-CoV-2) emerged causing severe respiratory illness and pneumonia. The continual emergence of novel coronaviruses underscores the importance of developing effective vaccines as well as novel therapeutic options that target either viral functions or host factors recruited to support coronavirus replication. The CoV nonstructural protein 1 (nsp1) has been shown to promote cellular mRNA degradation, block host cell translation, and inhibit the innate immune response to virus infection. Interestingly, deletion of the nsp1-coding region in infectious clones prevented the virus from productively infecting cultured cells. Because of nsp1’s importance in the CoV lifecycle, it has been highlighted as a viable target for both antiviral therapy and vaccine development. However, the fundamental molecular and structural mechanisms that underlie nsp1 function remain poorly understood, despite its critical role in the viral lifecycle. Here we report the high-resolution crystal structure of the amino, globular portion of SARS-CoV-2 nsp1 (residues 10 – 127) at 1.77Å resolution. A comparison of our structure with the SARS-CoV-1 nsp1 structure reveals how mutations alter the conformation of flexible loops, inducing the formation of novel secondary structural elements and new surface features. Paired with the recently published structure of the carboxyl end of nsp1 (residues 148 – 180), our results provide the groundwork for future studies focusing on SARS-CoV-2 nsp1 structure and function during the viral lifecycle.IMPORTANCEThe Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is the causative agent for the COVID-19 pandemic. One protein known to play a critical role in the coronavirus lifecycle is nonstructural protein1 (nsp1). As such, it has been highlighted in numerous studies as a target for both the development of antivirals and for the design of live-attenuated vaccines. Here we report the high-resolution crystal structure of nsp1 derived from SARS-CoV-2 at 1.77Å resolution. This structure will facilitate future studies focusing on understanding the relationship between structure and function for nsp1. In turn, understanding these structure-function relationships will allow nsp1 to be fully exploited as a target for both antiviral development and vaccine design.
Is the Healthy Start scheme associated with increased food expenditure in low-income families with young children in the United Kingdom?
Authors: Parnham Jennie , Millett Christopher , Chang Kiara , Laverty Anthony A , von Hinke Stephanie , Pearson-Stuttard Jonathan , Vamos Eszter P 
Published: November 2020

ABSTRACTIntroductionHealthy Start is a food assistance programme in the United Kingdom (UK) which aims to enable low-income families on welfare benefits to access a healthier diet through the provision of food vouchers. Healthy Start was launched in 2006 but remains under-evaluated. This study aims to determine whether participation in the Healthy Start scheme is associated with differences in food expenditure in a nationally representative sample of households in the UK.MethodsCross-sectional analyses of the Living Costs and Food Survey dataset (2010-2017). All households with a child (0-3 years) or pregnant woman were included in the analysis (n=4,869). Multivariable quantile regression compared the expenditure and quantity of fruit and vegetables (FV), infant formula and total food purchases. Four exposure groups were defined based on eligibility, participation and income (Healthy Start Participating, Eligible Non-participating, Nearly Eligible low-income and Ineligible high-income households).ResultsOf 876 eligible households, 54% participated in Healthy Start. No significant differences were found in FV or total food purchases between participating and eligible non-participating households, but infant formula purchases were lower in Healthy Start participating households. Ineligible higher-income households had higher purchases of FV.ConclusionThis study did not find evidence of an association between Healthy Start participation and FV expenditure. Moreover, inequalities in FV purchasing persist in the UK. Higher participation and increased voucher value may be needed to improve programme performance and counteract the harmful effects of poverty on diet.
The transition of spatial organisation planning of pre and post-colonial housing in Khartoum
Authors: Ibrahim Gadsiah M.A. , Saeed Tallal A. , El-Khouly Tamir 
Published: November 2020

PurposeThe focus of this paper is the changes in domestic social patterns from independence (1956) in Khartoum and their effects on domestic architecture, analysed using space syntax analysis. The built representation of the political change in Khartoum, which had led economic, cultural and social transformation, has not been well investigated. The domestic architecture is envisaged here to reflect the change from a liberal and secular British colonial lifestyle to a post-colonial native conservative and religious one.Design/methodology/approachThe study explored twenty representative samples from the two eras in order to reveal the hidden nature of these patterns by employing space syntax analysis, particularly convex mapping. This analysis attempted to both decipher space transition and to identify patterns. Interviews were conducted to interpret the social meaning of these configurations and to factor in the historical context of the transition.FindingsThe analytical comparisons revealed that these socio-cultural changes had subtle effects on the transformations experienced in the use and spatial organization. The change shows the emerging dominance of privacy: the relationship with exterior had started to diminish, and some interior spaces were redefined. This privacy centred patterns also, in many post-colonial cases, have pushed the core of integration deeper in the access graph.Social implicationsThe study sheds lights on the transition in the Sudanese society, reflected on the spatial arrangement of houses and traditions. It is directed to the Sudanese as well as regional societies who passed the transition before and after colonial eras.Originality/valueThe enclosed j-graph study on houses' plans is original and haven't been investigated using this space syntax approach.
The impact of firm size on competitive intelligence activities
Authors: Calof Jonathan 
Published: November 2020

Purpose
Given the importance of competitive intelligence (CI) to the economic performance of firms, understanding whether CI practice is impacted by firm size or by their awareness of CI maybe important when creating programs designed to improve firms’ CI performance. This paper aims to address this by examining the extent to which the CI practices of small and medium-sized enterprises (SMEs) and large firms differed using a sample of firms with knowledge/awareness of CI.

Design/methodology/approach
A survey was developed that included 10 CI organization questions and 67 CI process questions. The survey was sent to a sample with awareness/knowledge of CI – strategic and CI professionals (SCIP) members and individuals who had attended SCIP events T-tests were then used to compare the SME’s and large firms’ responses to the 10 CI organization and 67 CI process questions.

Findings
For firms with CI awareness/knowledge, the study results suggest that size has very little relationship with CI practice. Of the 10 CI organization variables, only two were significantly different between the SME’s and the large firms. Large firms had more full-time CI staff and were more likely to have a formal intelligence unit compared to the SME’s. Of the 67 CI process variables, only four were significantly different between the SME’s and the large firms. Large firms made more use of company intranet for distributing CI findings use business analytics software and use commercial databases for information than SME’s while the SME’s used social media, in particular Facebook more than large firms, in their competitive intelligence activities.

Originality/value
This study uses a sample frame of firms with CI awareness/knowledge in examining differences between SME’s and large firms CI practices.
The roles of learning orientation: structural determinants for improving knowledge sharing with committed and satisfied employees
Authors: Ro Yun Jeong , Yoo Myunghyun , Koo Yohan , Song Ji Hoon 
Published: November 2020

Purpose
The purpose of this paper is to examine the relationships between several perceptional and behavioral determinants of employees’ knowledge sharing. Based on the conceptual model of the current research, individuals’ learning orientation was theorized as playing a mediating role in the influences of employees’ satisfaction and organizational commitment on knowledge sharing in the Korean retail service industry.

Design/methodology/approach
To analyze variable relationships, a total of 868 cases, collected from the Korean retail service industry, were used. Structural equation modeling was used for analyzing structural influential relationships among the variables.

Findings
One of the most important findings is that learning orientation is the key factor influencing knowledge sharing. More specifically, the analysis demonstrated that job satisfaction and organizational commitment have a significant influence on knowledge sharing only through learning orientation in terms of its mediating effect. In other words, learning orientation is a facilitator enhancing knowledge sharing through motivation and intention. The findings of this study have theoretical implications. In addition, an individual’s job satisfaction and organizational commitment could lead to a more active learning orientation and eventually could enhance knowledge-sharing activities.

Practical implications
The findings show that learning orientation is the key factor influencing knowledge sharing. In other words, learning orientation is a facilitator enhancing knowledge sharing through motivation and intention. These findings provide practical implications for practitioners who attempt to promote knowledge sharing among employees.

Originality/value
Most studies have considered the variables of job satisfaction and employee commitment as outcome variables. However, in the research, this paper founds that satisfied and committed employees could be the most critical influencers for organization performance and knowledge improvement.
Relational impact of buyer–supplier dyads on sustainable purchasing and supply management: a proximity perspective
Authors: Houé Thierry , Duchamp David 
Published: November 2020

PurposeSustainable purchasing and supply management (SPSM) is a lever of sustainable development for companies and remains an interesting research issue that can be analysed from various perspectives. By considering the polymorphic concept of proximity as a theoretical support, this research studies the buyer–supplier dyad and aims to answer the following question. How does the diversity of buyer–supplier dyad relationships influence the SPSM of direct and indirect purchases within a manufacturing company?Design/methodology/approachThe authors used a qualitative approach founded on a matrix analysis to describe buyer–supplier relationships through the prism of the proximity concept. The field of study consists in a single case with embedded units including a manufacturing company and a sample of 13 suppliers of direct and indirect purchases.FindingsThe research reveals diverse combinations of proximities that characterise relationships between a buyer and suppliers of several purchasing categories. This diversity of relational contexts influences SPSM in different ways. The authors highlight three SPSM approaches labelled contractual, relational and embedded and describe practices carried out with suppliers as part of the different relational profiles.Research limitations/implicationsThe research is developed in a single perspective. To ensure that it can be generalised, it should be applied in other contexts supported by new case studies.Practical implicationsThe research provides practitioners with guidelines on building successful buyer–supplier partnerships in a sustainable view. The authors’ findings aid managerial decision-making by validating the necessity of adapting SPSM depending on buyer–supplier relational situations.Originality/valueThis paper offers an original study angle on buyer–supplier relationships based on a proximity analysis. The authors’ research confirms the variety of sustainable purchasing relationships underlined by the literature and can advance the portfolio approach to sustainable purchasing.
Challenging listening environments in higher education: an analysis of academic classroom acoustics
Authors: van den Heuij Kirsten , Goverts Theo , Neijenhuis Karin , Coene Martine 
Published: November 2020

PurposeAs oral communication in higher education is vital, good classroom acoustics is needed to pass the verbal message to university students. Non-auditory factors such as academic language, a non-native educational context and a diversity of acoustic settings in different types of classrooms affect speech understanding and performance of students. The purpose of this study is to find out whether the acoustic properties of the higher educational teaching contexts meet the recommended reference levels.Design/methodology/approachBackground noise levels and the Speech Transmission Index (STI) were assessed in 45 unoccupied university classrooms (15 lecture halls, 16 regular classrooms and 14 skills laboratories).FindingsThe findings of this study indicate that 41 classrooms surpassed the maximum reference level for background noise of 35 dB(A) and 17 exceeded the reference level of 40 dB(A). At five-meter distance facing the speaker, six classrooms indicated excellent speech intelligibility, while at more representative listening positions, none of the classrooms indicated excellent speech intelligibility. As the acoustic characteristics in a majority of the classrooms exceeded the available reference levels, speech intelligibility was likely to be insufficient.Originality/valueThis study seeks to assess the acoustics in academic classrooms against the available acoustic reference levels. Non-acoustic factors, such as academic language complexity and (non-)nativeness of the students and teaching staff, put higher cognitive demands upon listeners in higher education and need to be taken into account when using them in daily practice for regular students and students with language/hearing disabilities in particular.
Is there any effect of ESG scores on portfolio performance? Evidence from Europe and Turkey
Authors: Zehir Emre , Aybars Aslı 
Published: November 2020

PurposeThe purpose of this paper is to examine the performance of portfolios that are constructed based on environmental, social and governance (ESG) scores and consist of stocks located in Europe and Turkey.Design/methodology/approachIn order to form the portfolios, firstly all stocks are ranked in a descending way based on ESG-based (ESG, environmental, social and governance) scores, separately. Then, 10% of stocks with the highest scores are included in the “Top” portfolio and 10% of stocks with the lowest scores are included in “Bottom” portfolio and totally performance of eight portfolios are investigated. Finally, capital asset pricing model (CAPM) and Fama-French three-factor model are employed as performance measurement benchmarks.FindingsResults obtained from CAPM regression show that using ESG-based scores two portfolios underperform the market index. The results of the three-factor model provide that performances of Bottom ESG and Bottom GOV portfolios outperform the market excess return by 0.57% and 0.53%. The overall findings of this paper indicate that there is no relationship between socially responsible investment (SRI) and portfolio performance. These findings are in line with the efficient market hypothesis which indicates all information is reflected in prices.Originality/valueThe aim of the study is to provide insight on the question of “whether SRI has any effect on the portfolio performance”. As far as the literature review is concerned it is seen that this study provide additional insight by utilizing a longer time span together with data from numerous markets.