bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dual in vitro invasion/migration suppressing and tamoxifen response modulating effects of a recombinant anti‐ALCAM scFv on breast cancer cells
Authors: Darvishi Behrad , Salehi Malihe , Boroumandieh Saeedeh , Majidzadeh‐A Keivan , Jalili Neda , Moradi‐Kalbolandi Shima , Farahmand Leila 
Published: March 2020

It has been shown that overexpression of activated leukocyte cell adhesion molecule (ALCAM) is involved in development of resistance to tamoxifen therapy and promotion of cell invasion, migration and metastasis in ER+ breast cancer cells. Thus, we hypothesized that blockade of ALCAM interconnections with antibodies could be an effective approach for reversing mentioned negative events associated with ALCAM overexpression in breast cancer cells. Here, an anti‐ALCAM scFv was recombinantly expressed and used throughout study for examination of the putative anticancer effects of ALCAM blockade. The anti‐ALCAM scFv coding sequence was obtained from GenBank database and after addition of a 6× His‐tag moiety, signal peptide and flanking sequences, the whole construct was expressed in Escherichia coli. Tamoxifen resistant MCF7 cells were then pretreat for 24 hours with purified recombinant anti‐ALCAM scFv prior to administration of tamoxifen. In parallel, the cytotoxicity profile of anti‐ALCAM scFv and tamoxifen co‐treatments against tamoxifen resistant and sensitive MCF7 cell lines was also evaluated using CompuSyn software. The invasion/migration inhibitory effects of anti‐ALCAM scFv on MDA‐MB‐231 cells were also evaluated. Pretreatment with anti‐ALCAM scFv could successfully enhance anti‐proliferative effects of tamoxifen against resistant MCF‐7 cell lines. Furthermore, the combination of 19.2:1 of tamoxifen to anti‐ALCAM scFv demonstrated synergistic cell inhibitory effect against tamoxifen resistant MCF7 cell lines. Also, incubating MDA‐MB‐231 cell lines with anti‐ALCAM scFv resulted in a 30% and 25% reduction in number of invaded and migrated cells respectively. Overall, application of anti‐ALCAM scFv could significantly suppress cancer cells metastasis in vitro and modulate tamoxifen resistant ER+ MCF7 cell line's sensitivity to tamoxifen.Significance of the studyAcquisition of resistance to tamoxifen therapy is one of the major challenges associated with cancer chemotherapy, gradually turning a responsive tumour into a refractory more invasive one which ultimately ends in disease progression and relapse. Here, we reported expression of an anti‐ALCAM scFv, capable of increasing the sensitivity of tamoxifen resistant ER+ MCF‐7 cells to tamoxifen therapy following a 24‐hour pretreatment period. In addition, we demonstrated that the anti‐ALCAM scFv monotherapy was also capable of suppressing invasion and migration of MDA‐MB‐231 cells in Boyden chamber assays.
Tripartite motif protein 25 is associated with epirubicin resistance in hepatocellular carcinoma cells via regulating PTEN/AKT pathway
Authors: Yuan Peng , Zheng Aidong , Tang Qing 
Published: March 2020

AbstractTripartite motif protein 25 (TRIM25) expression was altered in various human cancers. Herein, we found that the expression of TRIM25 was elevated in hepatocellular carcinoma (HCC) tissues and cell lines. Knockdown of TRIM25 increased the sensitivity of HCC HepG2 cells to epirubicin (EPI), as indicated by reduced cell viability, enhanced cell apoptosis, and downregulated P‐glycoprotein (P‐gp) and multiple drug‐resistance protein 1 (MRP1). Moreover, TRIM25 knockdown strengthened the effects of EPI on phosphatase and tensin homolog (PTEN) and phosphorylated (p)‐AKT. However, overexpression of TRIM25 exerted an opposite effect, weakening the sensitivity of Huh7 to EPI, and obviously increasing PTEN and reducing p‐AKT. Most important, all the changes induced by TRIM25 overexpression in Huh7 were reversed with additional treatment of LY294002 (an AKT pathway inhibitor). Notably, coimmunoprecipitation experiments confirmed the interaction between TRIM25 and PTEN. Knockdown of TRIM25 resulted in reduced ubiquitination of PTEN protein. Collectively, our data suggested that TRIM25 enhanced EPI resistance via modulating PTEN/AKT pathway, and targeting TRIM25 may enhance the sensitivity of HCC cells toward chemotherapy drugs.
Quantitative flow ratio—Meta‐analysis and systematic review
Authors: Cortés Carlos , Carrasco‐Moraleja Manuel , Aparisi Alvaro , Rodriguez‐Gabella Tania , Campo Alberto , Gutiérrez Hipolito , Julca Fabián , Gómez Itziar , San Román Jose Alberto , Amat‐Santos Ignacio J. 
Published: March 2020

AbstractIntroductionDespite of the wide evidence of use fractional flow reserve (FFR), isolated angiography evaluation is still the main tool to indicate percutaneous coronary intervention. Quantitative flow ratio (QFR) is a new functional index to assess functional significance. Recently, few studies have showed the capacity of QFR to predict significance stenosis. The aim of this research has been to describe the evidence of QFR in this clinical setting, to analyze the global diagnosis accuracy of QFR versus FFR and to compare the difference in feasibility between retrospective and prospective analysis.Methods and resultsSystematic review of literature was performed. Eligible studies for the meta‐analysis were considered those directly evaluating de QFR versus FFR. Pooled values of diagnosis test and summary receiver operator curve were calculated. Main causes of not‐perform QFR analysis according to study design were also evaluated.Sixteen studies were included. Good correlation and agreement were showed. Global sensibility, specificity, PPV, and NPV were 0.84, 0.89, 0.80, and 0.92, respectively. Then, 18% of evaluated vessels could not be analyzed. Significant differences were found in the percentage of discarded vessels and the cause of nonperformed analysis between retrospective or prospective analysis.ConclusionsExcellent correlation and agreement between QFR and FFR was demonstrated. QFR assessment could be improved by its prospective analysis with a dedicated protocol.
Intraoperative considerations in a patient with chondroblastic osteosarcoma and saddle pulmonary embolism undergoing amputation
Authors: Smisson William B. , Vale Henrique , Chandrashekar Arvind , Hierlmeier Bryan J. 
Published: March 2020

AbstractIn patients presenting to the operating room with a saddle pulmonary embolism for noncardiac surgery, it is best to take a multidisciplinary approach and involve the cardiac and vascular surgeons.
Immune thrombocytopenic purpura caused by the over‐the‐counter weight supplement Root of Tejocote (Crataegus species)
Authors: Alhasson Hussam , Muchnik Eugene 
Published: March 2020

AbstractOver‐the‐counter supplements, such as Alipotec which often purported to amplify weight loss and readily available in naturopathic shops, can have clinically significant patient outcomes including severe cytopenia, and even inducing immune thrombocytopenic purpura.
CO2 Hydrogenation with Cu/ZnO/Al2O3: A Benchmark Study
Authors: Ruland Holger , Song Huiqing , Laudenschleger Daniel , Stürmer Sascha , Schmidt Stefan , He Jiayue , Kähler Kevin , Muhler Martin , Schlögl Robert 
Published: March 2020

AbstractThe suitability of a commercial and industrially applied Cu‐based catalyst for the synthesis of methanol by CO2 hydrogenation was investigated. Unexpectedly, this system showed high stability and well‐performance under conditions that may be relevant for chemical energy conversion using hydrogen and energy from renewable technologies. This Cu‐based catalyst demonstrated excellent suitability for dynamical process operation that may be essential for effective compensation of the volatility of renewable energy sources.
Analysis of Morphological Changes in Monosodium Urate Monohydrate Crystals for Gout Treatment
Authors: Oda Momoko , Hirasawa Izumi , Kohori Fukashi 
Published: March 2020

AbstractGout is arthritis induced by monosodium urate monohydrate (MSU) crystals. The treatment of gout is mainly a symptomatic treatment, by suppressing the blood uric acid concentration with drugs. Here, the changes in crystal morphology in the process of MSU crystallization were investigated. Experiments were conducted to clarify the mechanisms of nucleation and growth of the MSU crystals, revealing that MSU first precipitated as granular crystals. Thereafter, nucleation and growth of needle‐like crystals were observed concomitant with the disappearance of the granular crystals. These results suggest that it is necessary to suppress the nucleation of granular crystals and the change in morphology from granular to needle‐like of the MSU crystals that cause gout.
Electronic Consequences of Ligand Substitution at Heterometal Centers in Polyoxovanadium Clusters: Controlling the Redox Properties through Heterometal Coordination Number
Authors: Meyer Rachel L. , Anjass Montaha H. , Petel Brittney E. , Brennessel William W. , Streb Carsten , Matson Ellen M. 
Published: March 2020

AbstractThe rational control of the electrochemical properties of polyoxovanadate‐alkoxide clusters is dependent on understanding the influence of various synthetic modifications on the overall redox processes of these systems. In this work, the electronic consequences of ligand substitution at the heteroion in a heterometal‐functionalized cluster was examined. The redox properties of [V5O6(OCH3)12FeCl] (1‐[V5FeCl]) and [V5O6(OCH3)12Fe]X (2‐[V5Fe]X; X=ClO4, OTf) were compared in order to assess the effects of changing the coordination environment around the iron center on the electrochemical properties of the cluster. Coordination of a chloride anion to iron leads to an anodic shift in redox events. Theoretical modelling of the electronic structure of these heterometal‐functionalized clusters reveals that differences in the redox profiles of 1‐[V5FeCl] and 2‐[V5Fe]X arise from changes in the number of ligands surrounding the iron center (e.g., 6‐coordinate vs. 5‐coordinate). Specifically, binding of the chloride to the sixth coordination site appears to change the orbital interaction between the iron and the delocalized electronic structure of the mixed‐valent polyoxovanadate core. Tuning the heterometal coordination environment can therefore be used to modulate the redox properties of the whole cluster.
Mineralization Mechanism of Mineralization Bacteria on Strontium Crystallization of Simulated Radionuclides
Authors: Tian Xiuquan , Zhang Yingchun , Zhang Jian , Ye Zhiyang , Lian Jie , Duan Tao , He Rong , Zhu Wenkun 
Published: March 2020

AbstractMicrobial‐induced carbonate crystallization is a natural biological process in which microbes produce inorganic materials as part of their basic metabolic activities. Herein, the influence of the bacterial somatic solution and secretion solution on the crystal growth behavior of strontium carbonate (SrCO3) is studied. The mineralization mechanism of carbonate mineralizing bacteria on simulated radionuclides (Sr2+) is also demonstrated. The results reveal that the morphology of SrCO3 crystal formed by involvement of microbes is greatly different with chemical method, which means that microbes play a critical role during the SrCO3 crystallization process. Briefly, bacterial somatic provides nucleation sites for SrCO3 during both the nucleation and growth processes. In addition, bacterial secretions play a vital role in regulating the morphology of mineralized products. This work unveils the mineralization process of strontium nuclides, providing reference for the control of crystal morphologies and structural properties of the SrCO3.
The Nature of Carbon Dioxide in Bare Ionic Liquids
Authors: Zanatta Marcileia , Simon Nathália M. , Dupont Jairton 
Published: March 2020

AbstractIonic liquids (ILs) are among the most studied and promising materials for selective CO2capture and transformation. The high CO2sorption capacity associated with the possibility to activate this rather stable molecule through stabilization of ionic/radical species or covalent interactions either with the cation or anion has opened new avenues for CO2functionalization. However, recent reports have demonstrated that another simpler and plausible pathway is also involved in the sorption/activation of CO2by ILs associated with basic anions. Bare ILs or IL solutions contain almost invariable significant amounts of water and through interaction with CO2generate carbonates/bicarbonates rather than carbamic acids or amidates. In these cases, the IL acts as a base and not a nucleophile and yields buffer‐like solutions that can be used to shift the equilibrium toward acid products in different CO2reutilization reactions. In this Minireview, the emergence of IL buffer‐like solutions as a new reactivity paradigm in CO2capture and activation is described and analyzed critically, mainly through the evaluation of NMR data.
An automated patient monitoring using discrete‐time wireless sensor networks
Authors: Chatterjee Aditi , Biswas Jayabrata , Das Kiranmoy 
Published: March 2020

SummaryIn recent years, Internet of Things (IoT) devices are used for remote health monitoring. For remotely monitoring a patient, only the health information at different time points are not sufficient; predicted values of biomarkers (for some future time points) are also important. In this article, we propose a powerful statistical model for an efficient dynamic patient monitoring using wireless sensor nodes through Bayesian Learning (BL). We consider the setting where a set of correlated biomarkers are measured from a patient through wireless sensors, but the sensors only report the ordinal outcomes (say, good, fair, high, or very high) to the sink based on some prefixed thresholds. The challenge is to use the ordinal outcomes for monitoring and predicting the health status of the patient under consideration. We propose a linear mixed model where interbiomarker correlations and intrabiomarker dependence are modeled simultaneously. The estimated and the predicted values of the biomarkers are transferred over the internet so that health care providers and the family members of the patient can remotely monitor the patient. Extensive simulation studies are performed to assess practical usefulness of our proposed joint model, and the performance of the proposed joint model is compared to that of some other traditional models used in the literature.
Elevated pre‐transplant pulmonary vascular resistance is associated with early post‐transplant atrial fibrillation and mortality
Authors: Rivinius Rasmus , Helmschrott Matthias , Ruhparwar Arjang , Schmack Bastian , Darche Fabrice F. , Thomas Dierk , Bruckner Tom , Doesch Andreas O. , Katus Hugo A. , Ehlermann Philipp 
Published: March 2020

AbstractAimsSeverely elevated pre‐transplant pulmonary vascular resistance (PVR) has been linked to adverse effects after heart transplantation (HTX). The impact of a moderately increased PVR before HTX on post‐transplant outcomes remains uncertain. The aim of this study was to investigate the effects of an elevated pre‐transplant PVR ≥ 300 dyn·s·cm−5 (≥3.75 Wood units) on outcomes after HTX.Methods and resultsThis observational retrospective single‐centre study included 561 patients receiving HTX at Heidelberg Heart Center between 1989 and 2015. Patients were stratified by degree of pre‐transplant PVR. Analyses covered demographics, post‐transplant medication, mortality and causes of death after HTX, early post‐transplant atrial fibrillation (AF), and length of the initial hospital stay after HTX. Ninety‐four patients (16.8%) had a PVR ≥ 300 dyn·s·cm−5 (≥3.75 Wood units). These patients had a higher rate of early post‐transplant AF [20.2 vs. 10.7%, difference: 9.5%, 95% confidence interval (CI): 0.9–18.1%, P = 0.01] and an increased 30 day post‐transplant mortality (25.5 vs. 6.4%, hazard ratio: 4.4, 95% CI: 2.6–7.6, P < 0.01), along with a higher percentage of death due to transplant failure (21.2 vs. 4.1%, difference: 17.1%, 95% CI: 8.7–25.5%, P < 0.01). Multivariate analysis revealed a PVR ≥ 300 dyn·s·cm−5 (≥3.75 Wood units) as a significant risk factor for increased 30 day mortality after HTX (hazard ratio: 4.4, 95% CI: 2.5–7.6, P < 0.01). Kaplan–Meier estimator showed a lower 2 year survival after HTX (P < 0.01) in patients with a PVR ≥ 300 dyn·s·cm−5 (≥3.75 Wood units).ConclusionsElevated pre‐transplant PVR ≥ 300 dyn·s·cm−5 (≥3.75 Wood units) is associated with early post‐transplant AF and increased mortality after HTX.
Saw tooth cardiomyopathy: a case report
Authors: Chenaghlou Maryam , Kasaei Mohammad , Taghavi Sepideh , Amin Ahmad , Naderi Nasim 
Published: March 2020

AbstractSaw tooth cardiomyopathy is an unusual and rare type of left ventricular dysplasia that is characterized by multiple projections of compacted myocardium that makes the appearance of ‘saw tooth’ in noninvasive imaging. We present a young man with signs and symptoms of heart failure and reduced left ventricular function in echocardiography who showed distinctive left ventricle features of saw tooth cardiomyopathy (saw tooth appearance of myocardium in basal inferolateral and basal to mid lateral segments) in cardiac magnetic resonance imaging.
Nutritional and antinutritional evaluation of complementary foods formulated from maize, pea, and anchote flours
Authors: Gemede Habtamu Fekadu 
Published: March 2020

AbstractThis study was aimed to evaluate nutritional and antinutritional contents of complementary foods from locally available and affordable raw materials (maize, pea, and anchote) grown in Western Ethiopia. The six formulated complementary diets analyzed for their proximate, mineral, and antinutritional continents were compared with Codex standards. The mineral ratios and molar ratios of the formulated diets were also evaluated and compared with each standard values. Six formulations were generated by d‐optimal mixture design. The formulated ingredient ranges 45%–61% maize, 23%–31% pea, and 14%–28% anchote. Design‐Expert® 6 (Stat‐Ease) was used to constrain the three components. The formulated diets ranged from 14.92% to 20.99%, 5.95% to 9.94%, 2.75% to 3.41%, and 59.10% to 66.22% of protein, fat, fiber, and utilizable carbohydrate, respectively. Mineral contents (mg/100 g) of the formulated diet ranged from 225.45 to 261.32, 11.48 to 12.61, 2.73 to 3.00, 357.92 to 391.13, 298.55 to 332.63, 252.00 to 278.01, and 44.26 to 51.56 for calcium, iron, zinc, phosphorous, potassium, sodium and magnesium, respectively. The proximate and mineral contents of the formulated diet 5 meet the Codex standards, except the fat contents of the complementary food standards. The molar ratios of the formulated diets in this study were below standard reference and which show the high mineral bioavailability in all the formulated diets. The results of the study revealed that the formulated diets contain very low antinutritional factors and high mineral bioavailability. The paper's findings show that the complementary food formulated from maize, pea, and anchote flours particularly diet 5 may be suitable to alleviate protein energy malnutrition and it can be used as a substitute for the expensive commercial complementary food.
Real world outcomes with Bortezomib Thalidomide dexamethasone and Cyclophosphamide Bortezomib dexamethasone induction treatment for transplant eligible multiple myeloma patients in a Latin American country. A Retrospective Cohort Study from Grupo Argentino de Mieloma Múltiple
Authors: Schütz Natalia P. , Ochoa Paola , Duarte Patricio , Remaggi Guillermina , Yantorno Sebastián , Corzo Ariel , Zabaljauregui Soledad , Shanley Claudia , Lopresti Sergio , Orlando Sergio , Verri Verónica , Quiroga Luis , García Carlos A. , Fernández Vanesa , Fantl Dorotea 
Published: March 2020

AbstractData about treatment outcomes and toxicity in Latin America are scarce. There are differences with central countries based on access to healthcare system and socioeconomic status. Argentinean Society of Hematology recommends bortezomib‐based triplets for induction treatment of transplant eligible newly diagnosed multiple myeloma patients. Most common options are CyBorD (cyclophosphamide, bortezomib and dexamethasone) and VTD (bortezomib, thalidomide and dexamethasone). Main goal of our retrospective, multicentric study was to compare very good partial response rate (VGPR) or better after induction treatment in a real‐world setting in Argentina. Secondary objectives included comparison of complete response (CR) post‐induction and after bone marrow transplantation, grade 3‐4 adverse events (AEs), progression‐free survival (PFS) and overall survival (OS). Three hundred twenty‐two patients were included (median age at diagnosis: 57 years; 52% male; 28% had ISS3; 14% with high‐risk cytogenetics; median follow up: 34 months). CyBorD was indicated in 74% and 26% received VTD. In VTD arm, 72.62% of patients achieved at least VGPR vs 53.36% receiving CyBorD (odds ratio, OR: 1.96 [95% confidence interval, CI: 1.08‐3.57; P = .026] after adjusting by age, ISS [International Staging System], lactate dehydrogenase levels (LDH) and cytogenetic risk. Difference in VGPR was 19.26% (95% CI: 15‐24). CR rate were 35.92% (VTD) vs 22.55% (CyBorD) (adjusted OR: 2.13 [95% CI: 1.12‐4.05]). Difference in CR was 13.37% (95% CI: 9.6‐17.53). Adverse events (AEs) were more common with VTD (69.05% vs 55.46% for CyBorD; P = .030), especially grade 3‐4 neuropathy (P = .005) and thrombosis (P = .001). Thromboprophylaxis was inadequate in 20.24% of patients. Hematological AEs were more common with CyBorD, especially thrombocytopenia (P = .017). PFS and OS at 24 months were not different between treatments. In this real‐world setting, VTD was associated with better CR and VGPR than CyBorD. Nevertheless, CyBorD continues to be the preferred induction regimen in Argentina, based on safety profile. Frontline autologous stem cell transplantation improves quality of responses, especially in countries with limited access to new drugs.
Safety and efficacy of ischial spine fascia and sacrospinous ligament fixation for symptomatic stage 2 and 3 apical prolapse
Authors: Ren Chang , Zhu Lan 
Published: March 2020

AbstractObjectiveTo compare safety, efficacy, and impact on quality of life (QoL) between ischial spine fascia fixation (ISFF) and sacrospinous ligament fixation (SSLF) for stage 2–3 pelvic organ prolapse (POP).MethodA prospective cohort study among women with POP attending a national referral university hospital in Beijing, China, between May 2007 and May 2015. Women underwent either ISFF or SSLF. Primary end point was objective success rates at 3 months after surgery. Exploratory outcomes included perioperative parameters, complications, subjective satisfaction rates and QoL at 1 year.ResultsThere was no difference in baseline characteristics between the groups (all P>0.05). After ISFF and SSLF, the objective success rate at 3 months was 100% and 98.1% (P>0.99), the recurrence rate at 1 year was 5.3% and 8.3% (P=0.266), and the subjective satisfaction rate at 1 year was 97.8% and 97.9%, respectively. Pelvic Organ Prolapse/Urinary Incontinence Sexual Questionnaire‐12 scores improved significantly after ISFF. De novo urinary incontinence occurred for 5.3% and 6.3% of women, respectively, and de novo dyspareunia for approximately 14% of women in both groups.ConclusionISFF was found to be a safe and effective alternative to SSLF for women with symptomatic stage 2 and 3 POP.