bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of Motivation for the Treatment of Drug Addicts with Personality Disorders
Authors: Martínez-González José M. , Caracuel Alfonso , Vilar-López Raquel , Becoña Elisardo , Verdejo-García Antonio 
Published: June 2020

Abstract
Lack of motivation for the treatment of drug addiction is associated with dropout and relapses. Further, personality disorders (PD) have traditionally been linked to low motivation and therapeutic failure. Thus, the present study aims to analyze the structure of the Motivation for Treatment Questionnaire (MTQ–8), as well as to determine differences in motivation due to the presence of PD and the impact of psychological adjustment on motivation. The sample included 125 patients (84% male) who started a treatment for their addiction to cocaine and alcohol. Rasch analysis was applied for the first objective, and means contrast and regression analysis for the others. The two subscales of the MTQ–8 fit the Rasch model, with appropriate psychometric characteristics when merging Items 5 and 7. The presence of PD was not associated with reduced motivation. Motivation for treatment was greater when abstinence was less than three weeks, and psychological distress predicted motivation for treatment. The present study confirms that MTQ–8 subscales are suitable for measuring motivation for treatment and readiness for change in drug-dependent patients. It is noted that the presence of PD should not be associated with a lower level of motivation, and that psychological distress influences motivation.
Psychological and Sociodemographic Variables related to Cortisol in Hair in Spanish Healthy Population
Authors: García Laura Arco , Pérez Raquel González , Extremera Alejandra Henares , León María Ángeles García , Peralta-Ramírez María Isabel 
Published: June 2020

AbstractSome recent researches have shown the important role of hair cortisol as a retrospective biomarker of chronic stress. The aim of this research was to investigate the relationship between hair cortisol levels and sociodemographic and psychological variables, such as perceived stress levels and psychopathological symptoms on a Spanish population. The sample consisted of 347 healthy people, 230 women and 117 men, with an average age of 33.39 years (SD= 12.63). Hair cortisol levels were measured by obtaining a hair sample. In addition, a psychological assessment composed by: Analogic-Visual Stress Scale, Perceived Stress Scale (PSS), Symptom Checklist 90 Revised (SCL–90–R) and the assessment of vital stressful events suffered, was carried out. The mean cortisol level was 108.93 pg/mg (SD= 66.43) in men, and 120.38 pg/mg (SD= 87.26) in women. The linear hierarchical regression showed that Analogic-Visual Stress Scale and perceived stress levels were related with higher hair cortisol levels (R2= .032;t= 2.21;p= .029). Due to the relationship between daily stress levels, Analogic-Visual Stress Scale, anxiety sub-scale of SCL 90–R and perceived stress levels with hair cortisol levels, we conclude that there is a relation between perceived yourself stressed and the physiological levels.
High‐Resolution 3‐D Shear Wave Velocity Model of the Tibetan Plateau: Implications for Crustal Deformation and Porphyry Cu Deposit Formation
Authors: Huang Shuye , Yao Huajian , Lu Zhanwu , Tian Xiaobo , Zheng Yong , Wang Rui , Luo Song , Feng Jikun 
Published: June 2020

AbstractThe high topography of the Tibetan Plateau was generated by the Cenozoic India‐Eurasia collision. A high‐resolution shear wave velocity model can provide improved understanding of the Tibetan structure and crustal deformation with complicated tectonic evolution. Based on continuous seismic observations at approximately 400 stations, we collected over 10,000 Rayleigh wave phase velocity dispersion curves extracted from ambient noise cross‐correlation functions. A direct surface wave inversion method was applied to obtain an S wave velocity model of the Tibetan crust. A heterogeneous structure including several prominent low‐velocity zones (LVZs) and relatively narrow low‐velocity bands connecting the LVZs is revealed. Our model shows significant crustal low‐velocity structures with lateral variations along the Himalayan front. In the eastern segment of the Lhasa terrane, the LVZ is spatially correlated with Miocene porphyry Cu deposits, which are probably related to strong tearing of the Indian lithosphere, while preexisting weak zones contribute to the LVZs in the western segment. Meanwhile, the LVZ along the Bangong‐Nujiang suture could be considered as a channel for eastward extrusion of ductile material, that is, crustal flow on geological timescales. This “flow” in the middle‐lower crust probably contributed to the formation of the V‐shaped conjugate strike‐slip fault system in central Tibet. The development of conjugate strike‐slip faults, however, ceased near 90°E, since the eastward “flow” was blocked by an intracrustal high‐velocity block in Amdo. This preexisting rigid zone (containing the Amdo microcontinent) hence influences the pattern of material transport inside the Tibetan crust and the deformation of the plateau.
Erosion of the Subsurface Salinity Maximum of the Loop Current Eddies From Glider Observations and a Numerical Model
Authors: Sosa‐Gutiérrez R. , Pallàs‐Sanz E. , Jouanno J. , Chaigneau A. , Candela J. , Tenreiro M. 
Published: June 2020

AbstractThe erosion of the subsurface salinity maximum, signature of the Caribbean Subtropical UnderWater (SUW), within the Loop Current Eddy (LCE) Poseidon (August 2016 to July 2017) in the Gulf of Mexico (GoM) and the formation of the Gulf Common Water (GCW) during its journey westward, was observed using glider data. Most of the dilution of the SUW high‐salinity core within Poseidon occurs during late autumn and winter associated with Northern winds and mixed‐layer deepening. The physical processes that contribute to salt dilution of the SUW inside the LCEs' core are investigated using a numerical regional model. The analysis of the salt budget in a long‐lasting numerical LCE and a composite analysis of sixteen LCEs reveal that the salinity trend is mostly explained by the vertical salinity diffusion. Cold and dry Northern winds during the first winter drive strong negative net heat fluxes that trigger turbulent flux of salt into the LCEs' thermostad and dilution of the SUW high‐salinity core below. The vertical salinity diffusion continues homogenizing the salinity in the upper ocean until the vertical gradient of salinity is negligible. As a result, SUW is transformed to precursor of GCW that is ultimately diffused to surrounding waters in the western GoM. Although the contribution of advection to the salinity trend above the isopycnal of 1,026 kg m−3 is of second order, below is the most important process driving loss of salinity, presumably due to, eddy‐eddy interactions during LCEs' westward propagation and eddy pumping (upwelling) during the LCEs' decaying phase along the western slope.
The Prevalence and Significance of Offset Magma Reservoirs at Arc Volcanoes
Authors: Lerner Allan H. , O'Hara Daniel , Karlstrom Leif , Ebmeier Susanna K. , Anderson Kyle R. , Hurwitz Shaul 
Published: June 2020

AbstractDetermining the spatial relations between volcanic edifices and their underlying magma storage zones is fundamental for characterizing long‐term evolution and short‐term unrest. We compile centroid locations of upper crustal magma reservoirs at 56 arc volcanoes inferred from seismic, magnetotelluric, and geodetic studies. We show that magma reservoirs are often horizontally offset from their associated volcanic edifices by multiple kilometers, and the degree of offset broadly scales with reservoir depth. Approximately 20% of inferred magma reservoir centroids occur outside of the overlying volcano's mean radius. Furthermore, reservoir offset is inversely correlated with edifice size. Taking edifice volume as a proxy for long‐term magmatic flux, we suggest that high flux or prolonged magmatism leads to more centralized magma storage beneath arc volcanoes by overprinting upper crustal heterogeneities that would otherwise affect magma ascent. Edifice volumes therefore reflect the spatial distribution of underlying magma storage, which could help guide monitoring strategies at volcanoes.
Emissions and exposures of graphene nanomaterials, titanium dioxide nanofibers, and nanoparticles during down-stream industrial handling
Authors: Lovén Karin , Franzén Sara M. , Isaxon Christina , Messing Maria E. , Martinsson Johan , Gudmundsson Anders , Pagels Joakim , Hedmer Maria ,   NanoLund, Lovén Karin , Franzén Sara M. , Isaxon Christina , Messing Maria E. , Gudmundsson Anders , Pagels Joakim , Hedmer Maria 
Published: June 2020

AbstractToday, engineered nanomaterials are frequently used. Nanosized titanium dioxide (TiO2) has been extensively used for many years and graphene is one type of emerging nanomaterial. Occupational airborne exposures to engineered nanomaterials are important to ensure safe workplaces and to extend the information needed for complete risk assessments. The main aim of this study was to characterize workplace emissions and exposure of graphene nanoplatelets, graphene oxide, TiO2 nanofibers (NFs) and nanoparticles (NPs) during down-stream industrial handling. Surface contaminations were also investigated to assess the potential for secondary inhalation exposures. In addition, a range of different sampling and aerosol monitoring methods were used and evaluated. The results showed that powder handling, regardless of handling graphene nanoplatelets, graphene oxide, TiO2 NFs, or NPs, contributes to the highest particle emissions and exposures. However, the exposure levels were below suggested occupational exposure limits. It was also shown that a range of different methods can be used to selectively detect and quantify nanomaterials both in the air and as surface contaminations. However, to be able to make an accurate determination of which nanomaterial that has been emitted a combination of different methods, both offline and online, must be used.
The deubiquitylase UCHL3 maintains cancer stem-like properties by stabilizing the aryl hydrocarbon receptor
Authors: Ouyang Lianlian , Yan Bin , Liu Yating , Mao Chao , Wang Min , Liu Na , Wang Zuli , Liu Shouping , Shi Ying , Chen Ling , Wang Xiang , Cheng Yan , Cao Ya , Xiao Desheng , Zhang Lingqiang , Liu Shuang , Tao Yongguang 
Published: June 2020

AbstractCancer stem cells (CSCs) exhibit highly aggressive and metastatic features and resistance to chemotherapy and radiotherapy. Aryl hydrocarbon receptor (AhR) expression varies among non-small cell lung cancers (NSCLCs), and the mechanisms that support abnormal AhR expression in CSCs remain elusive. Here, we identified ubiquitin carboxyl terminal hydrolase L3 (UCHL3), a DUB enzyme in the UCH protease family, as a bona fide deubiquitylase of the AhR in NSCLC. UCHL3 was shown to interact with, deubiquitylate, and stabilize AhR in a manner dependent on its deubiquitylation activity. Moreover, we showed that UCHL3 promotes the stem-like characteristics and potent tumorigenic capacity of NSCLC cells. UCHL3 increased AhR stability and the binding of AhR to the promoter regions of the “stemness” genes ATP-binding cassette subfamily G member 2 (ABCG2), KLF4, and c-Myc. Depletion of UCHL3 markedly downregulated the “stemness” genes ABCG2, KLF4, and c-Myc, leading to the loss of self-renewal and tumorigenesis in NSCLCs. Furthermore, the UCHL3 inhibitor TCID induced AhR degradation and exhibited significantly attenuated efficacy in NSCLC cells with stem cell-like properties. Additionally, UCHL3 was shown to indicate poor prognosis in patients with lung adenocarcinoma. In general, our results reveal that the UCHL3 deubiquitylase is pivotal for AhR protein stability and a potential target for NSCLC-targeted therapy.
MicroRNA-17-5p regulates EMT by targeting vimentin in colorectal cancer
Authors: Kim Tae Won , Lee Yeo Song , Yun Nak Hyeon , Shin Chang Hoon , Hong Hye Kyung , Kim Hyeon Ho , Cho Yong Beom 
Published: June 2020

Abstract
Background
Epithelial–mesenchymal transition (EMT) is the most common cause of death in colorectal cancer (CRC). In this study, we investigated the functional roles of miRNA-17-5p in EMT of CRC cells.

Methods
In order to determine if miRNA-17-5p regulated EMT, the precursors and inhibitors of miR-17-5p were transduced into four CRC cells. To evaluate the regulatory mechanism, we performed argonaute 2 (Ago2) immunoprecipitation (IP) and luciferase assay. In addition, we used an intra-splenic injection mouse model of BALB/c nude mice to investigate the metastatic potential of miRNA-17-5p in vivo.

Results
The miRNA-17-5p expression was lower in primary CRC tissues with metastasis than in primary CRC tissues without metastasis in our RNA sequencing data of patient tissue. Real-time quantitative PCR revealed that miRNA-17-5p was inversely correlated with that of vimentin in five CRC cell lines. Over-expression of miRNA-17-5p decreased vimentin expression and inhibited cell migration and invasion in both LoVo and HT29 cells. However, inhibition of miRNA-17-5p showed the opposite effect. Ago2 IP and luciferase assay revealed that miRNA-17-5p directly bound to the 3′UTR of VIM mRNA. Furthermore, miRNA-17-5p inhibited the metastasis of CRC into liver in vivo.

Conclusions
Our results demonstrated that miRNA-17-5p regulates vimentin expression, thereby regulating metastasis of CRC.
Intratumoral immune activation with TLR4 agonist synergizes with effector T cells to eradicate established murine tumors
Authors: Albershardt Tina C. , Leleux Jardin , Parsons Andrea J. , Krull Jordan E. , Berglund Peter , ter Meulen Jan 
Published: June 2020

AbstractEffective T cell-based immunotherapy of solid malignancies requires intratumoral activity of cytotoxic T cells and induction of protective immune memory. A major obstacle to intratumoral trafficking and activation of vaccine-primed or adoptively transferred tumor-specific T cells is the immunosuppressive tumor microenvironment (TME), which currently limits the efficacy of both anti-tumor vaccines and adoptive cell therapy (ACT). Combination treatments to overcome TME-mediated immunosuppression are therefore urgently needed. We combined intratumoral administration of the synthetic toll-like receptor 4 agonist glucopyranosyl lipid A (oil-in-water formulation, G100) with either active vaccination or adoptive transfer of tumor-specific CD8 T cells to mice bearing established melanomas or orthotopically inoculated glioblastomas. In combination with cancer vaccines or ACT, G100 significantly increased expression of innate immune genes, infiltration and expansion of activated effector T cells, antigen spreading, and durable immune responses. Complete tumor regression of both injected and non-injected tumors was observed only in mice receiving combination immunotherapy. TLR4-based intratumoral immune activation may be a viable approach to enhance the efficacy of therapeutic cancer vaccines and ACT in patients.
Drop-on-demand cell bioprinting via Laser Induced Side Transfer (LIST)
Authors: Ebrahimi Orimi Hamid , Hosseini Kolkooh Sayadeh Sara , Hooker Erika , Narayanswamy Sivakumar , Larrivée Bruno , Boutopoulos Christos 
Published: June 2020

AbstractWe introduced and validated a drop-on-demand method to print cells. The method uses low energy nanosecond laser (wavelength: 532 nm) pulses to generate a transient microbubble at the distal end of a glass microcapillary supplied with bio-ink. Microbubble expansion results in the ejection of a cell-containing micro-jet perpendicular to the irradiation axis, a method we coined Laser Induced Side Transfer (LIST). We show that the size of the deposited bio-ink droplets can be adjusted between 165 and 325 µm by varying the laser energy. We studied the corresponding jet ejection dynamics and determined optimal conditions for satellite droplet-free bioprinting. We demonstrated droplet bio-printing up to a 30 Hz repetition rate, corresponding to the maximum repetition rate of the used laser. Jet ejection dynamics indicate that LIST can potentially reach 2.5 kHz. Finally, we show that LIST-printed human umbilical vein endothelial cells (HUVECs) present negligible loss of viability and maintain their abilities to migrate, proliferate and form intercellular junctions. Sample preparation is uncomplicated in LIST, while with further development bio-ink multiplexing can be attained. LIST could be widely adapted for applications requiring multiscale bioprinting capabilities, such as the development of 3D drug screening models and artificial tissues.
Identification and expression of DoCCaMK during Sebacina sp. symbiosis of Dendrobium officinale
Authors: Xing Yong-Mei , Zhao Ming-Ming , Guo Li-Cheng , Li Bing , Chen Juan , Guo Shun-Xing 
Published: June 2020

AbstractDendrobium officinale Kimura et Migo is a famous precious medicinal plant in China. Seed and seedling were cultivated with the mycorrhizal fungus Sebacina sp. CCaMK was initially cloned from D. officinale based on a SSH cDNA library of symbiotically germinated seeds with Sebacina sp. Phylogenetic analysis was performed among DoCCaMK and other CCaMKs. The particle bombardment technique was used to visualize DoCCaMK-GFP. qRT-PCR and western blot analysis were conducted to determine the tissue expression patterns of DoCCaMK with (SGS) and without (UGS) Sebacina sp. Furthermore, the effect of KN-93 on CCaMK expression was also examined. Using NMT the net Ca2+ fluxes and the CCaMK concentration were measured during D. officinale seed germination. DoCCaMK had the highest homology with Lilium longiflorum CCaMK. The DoCCaMK-GFP protein localized in the nucleus and cell membrane. CCaMK expression was significantly upregulated after symbiosis with Sebacina sp. KN-93 could be used as an inhibitor of CCaMK to inhibit D. officinale seed germination. Ca2+ influx and the concentration of the CCaMK in the SGS group was significantly more than that of the UGS group. The characterization of CCaMK provides certain genetic evidence for the involvement of this gene during seed germination and mycorrhizal cultivation in D. officinale.
Waiting-time statistics in magnetic systems
Authors: de Sousa Ivandson Praeiro , dos Santos Lima Gustavo Zampier , Correa Marcio Assolin , Sommer Rubem Luis , Corso Gilberto , Bohn Felipe 
Published: June 2020

AbstractMany complex systems, from earthquakes and financial markets to Barkhausen effect in ferromagnetic materials, respond with a noise consisting of discrete avalanche-like events with broad range of sizes and durations, separated by waiting times. Here we focus on the waiting-time statistics in magnetic systems. By investigating the Barkhausen noise in amorphous and polycrystalline ferromagnetic films having different thicknesses, we uncover the form of the waiting-time distribution in time series recorded from the irregular and irreversible motion of magnetic domain walls. Further, we address the question of if the waiting-time distribution evolves with the threshold level, as well as with the film thickness and structural character of the materials. Our results, besides informing on the temporal avalanche correlations, disclose the waiting-time statistics in magnetic systems also bring fingerprints of the universality classes of Barkhausen avalanches and a dimensional crossover in the domain wall dynamics.
Microvascular disease in chronic kidney disease: the base of the iceberg in cardiovascular comorbidity
Authors: Querfeld Uwe , Mak Robert H. , Pries Axel Radlach 
Published: June 2020

Abstract
Chronic kidney disease (CKD) is a relentlessly progressive disease with a very high mortality mainly due to cardiovascular complications. Endothelial dysfunction is well documented in CKD and permanent loss of endothelial homeostasis leads to progressive organ damage. Most of the vast endothelial surface area is part of the microcirculation, but most research in CKD-related cardiovascular disease (CVD) has been devoted to macrovascular complications. We have reviewed all publications evaluating structure and function of the microcirculation in humans with CKD and animals with experimental CKD. Microvascular rarefaction, defined as a loss of perfused microvessels resulting in a significant decrease in microvascular density, is a quintessential finding in these studies. The median microvascular density was reduced by 29% in skeletal muscle and 24% in the heart in animal models of CKD and by 32% in human biopsy, autopsy and imaging studies. CKD induces rarefaction due to the loss of coherent vessel systems distal to the level of smaller arterioles, generating a typical heterogeneous pattern with avascular patches, resulting in a dysfunctional endothelium with diminished perfusion, shunting and tissue hypoxia. Endothelial cell apoptosis, hypertension, multiple metabolic, endocrine and immune disturbances of the uremic milieu and specifically, a dysregulated angiogenesis, all contribute to the multifactorial pathogenesis. By setting the stage for the development of tissue fibrosis and end organ failure, microvascular rarefaction is a principal pathogenic factor in the development of severe organ dysfunction in CKD patients, especially CVD, cerebrovascular dysfunction, muscular atrophy, cachexia, and progression of kidney disease. Treatment strategies for microvascular disease are urgently needed.
A Multidisciplinary Delphi Consensus Study of Communicative Participation in Young Children With Language Disorders
Authors: Singer Ingrid , Klatte Inge S. , Welbie Marlies , Cnossen Ingrid C. , Gerrits Ellen 
Published: June 2020

PurposeOur aim was to develop consensus on the definition and operationalization of communicative participation (CP) in 2- to 8-year-old children with language disorders (LDs). A clear definition and operationalization can facilitate the discussion about children's communication problems in daily life between parents and professionals.MethodIn an online Delphi study, anonymized thoughts and opinions were collected on the definition and operationalization of CP in young children with LD. The 47 Delphi panel members were Dutch parents, young adults with LDs, teachers and assistants, speech-language pathologists, clinical linguists, and clinical researchers. Thematic content analysis was used to develop a concept definition and items operationalizing CP. The Delphi panel rated the suitability of concept definitions using a 7-point Likert scale. Concept definitions were revised with feedback from the Delphi panel until consensus was achieved. The Delphi panel rated items on how well they operationalize CP, using the same Likert scale.ResultsThe majority (79%) of the Delphi panel indicated that the essence of CP was captured by the definition: “CP is understanding and being understood in a social context, by applying verbal and non-verbal communication skills.” In addition, 33 behavioral items were developed.ConclusionThis study resulted in strong consensus on the definition of CP between Dutch parents and professionals. Items were developed that can inform speech-language pathologists on the type of questions to ask a child's parents or teacher when discussing CP. Further research is needed on how the items can best be used in clinical practice.