bluuebunny/crossref_metadata_2025_split huggingface dataset:
Characteristics of Planococcus antioxidans sp. nov., an antioxidant‐producing strain isolated from the desert soil in the Qinghai–Tibetan Plateau
Authors: Zhang Binglin , Yang Ruiqi , Zhang Gaosen , Liu Yang , Zhang Dongming , Zhang Wei , Chen Tuo , Liu Guangxiu 
Published: March 2020

AbstractStrain Y74T was an isolate from the sandy soil in the town of Huatugou, Qinghai–Tibet Plateau, China. An analysis of this strain's phenotypic, chemotaxonomic, and genomic characteristics established the relationship of the isolate with the genus Planococcus. Strain Y74T was able to grow between 4 and 42°C (with an optimum temperature of 28°C) at pH values of 6–8.5 and in 0%–7% (w/v) NaCl. The dominant quinones were MK‐8 and MK‐7. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, and an unknown phospholipid. The majority of the fatty acid content was anteiso‐C15:0 (28.8%) followed by C16:1 ω7c alcohol (20.9%) and iso‐C14:0 (13.4%). The 16S rRNA gene sequence similarity analysis demonstrated a stable branch formed by strain Y74T and Planococcus halotolerans SCU63T (99.66%). The digital DNA–DNA hybridization between these two strains was 57.2%. The G + C content in the DNA of Y74T was 44.5 mol%. In addition, the morphological, physiological, and chemotaxonomic pattern clearly differentiated the isolates from their known relatives. In conclusion, the strain Y74T (=JCM 32826T = CICC24461T) represents a novel member of the genus Planococcus, for which the name Planococcus antioxidans sp. nov. is proposed. Strain Y74T was found to have potent antioxidant activity via its hydrogen peroxide tolerance and its 1,1‐diphenyl‐2‐picrylhydrazyl (DPPH) radical‐scavenging activity. The DPPH radical‐scavenging activity was determined to be 40.2 ± 0.7%. The genomic analysis indicated that six peroxidases genes, one superoxide dismutase gene, and one dprA (DNA‐protecting protein) are present in the genome of Y74T.
Functionalization of silicon dioxide and silicon nitride surfaces with aminosilanes for optical biosensing applications
Authors: Antoniou Maria , Tsounidi Dimitra , Petrou Panagiota S. , Beltsios Konstantinos G. , Kakabakos Sotirios E. 
Published: March 2020

AbstractThe development of optical biosensors based on silicon dioxide or silicon nitride transducers requires the chemical activation of their surface to achieve stable, repeatable and homogeneous binding of biomolecules. In the present study, the chemical activation of silicon dioxide and silicon nitride surfaces with 3‐aminopropyl‐triethoxysilane (APTES) was optimized so as to enable the immobilization of biomolecules by adsorption or covalent bonding. Chemical activation was performed with either aqueous or organic solution of APTES, and the surfaces were used to immobilize directly protein molecules by physical adsorption or further modified with glutaraldehyde to allow covalent binding of protein molecules. The protein immobilization capacity of the chemically activated silicon dioxide and silicon nitride surfaces was evaluated through incubation with mouse γ‐globulins and reaction with a fluorescently labelled goat antimouse IgG antibody. By determining the surface fluorescence signal intensity, it was found that modification with 5% (v/v) APTES solution in ethanol followed by modification with glutaraldehyde provided 30% higher fluorescence signals than all the other protocols tested. In addition, this method provided the lower signal variation between different chips. To test the possible advantages of the chemical activation protocols for optical biosensing applications, they were also applied to a label‐free white light interference spectroscopy sensor and evaluated through (a) real‐time monitoring of the reaction between immobilized on the sensor surface mouse γ‐globulins with an unlabelled goat antimouse IgG antibody and (b) a non‐competitive immunoassay for the determination of C‐reactive protein. The results showed that in case of antibody, physical absorption provided marginally higher binding capacity to covalent bonding.
Changes in the climatic growing season in western Anatolia, Turkey
Authors: Arslantaş Ebru E. , Yeşilırmak Ercan 
Published: March 2020

AbstractIn this study spatial and temporal variations of climatic growing season parameters in western Anatolia, Turkey, during 1966–2015 are analysed. The beginning of the growing season (end of the growing season) for all threshold values of 5, 7 and 10°C is delayed (advances) from the southwest to the northeast over the study area. The growing season length (GSL) increases from northeast to southwest. The growing season start (GSS), growing season end (GSE) and GSL are strongly correlated with elevation, low‐elevation stations having earlier GSS, later GSE and longer GSL than stations at higher elevations. The regional average GSS shifted 7.3, 10.0 and 10.5 days earlier, while the regional average GSE moved 4.8, 6.1 and 6.2 days later, increasing the GSL by 12.0, 16.6 and 15.9 days for 5, 7 and 10°C, respectively. A comparison of mean values between subperiods documented significant changes at more than 50% of stations for GSS and GSL from the subperiod 1977–1997 to the subperiod 1998–2015 and almost no significant change between 1966–1976 and 1977–1997 for all thresholds. Correlation analysis showed that the East Atlantic Pattern was the main driver for GSS and the North Sea–Caspian Pattern for GSE, followed by the East Atlantic/Western Russia Pattern and the North Atlantic Oscillation, respectively. Monthly and annual precipitation totals and precipitation distribution within a year have not changed significantly, suggesting that air temperature has become more influential on GSS and GSE.
RETRACTED: Population genetic analysis of Shaanxi male Han Chinese population reveals genetic differentiation and homogenization of East Asians
Authors: Li Luyao , Zou Xing , Zhang Guanjun , Wang Hongyan , Su Yongdong , Wang Mengge , He Guanglin 
Published: March 2020

AbstractBackgroundShaanxi province, located in the upper Yellow River, has been evidenced as the geographic origin of Chinese civilization, Sino‐Tibetan‐speaking language, and foxtail or broomcorn millet farmers via the linguistic phylogenetic spectrum, archeological documents, and genetic evidence. Nowadays, Han Chinese is the dominant population in this area. The formation process of modern Shaanxi Han population reconstructed via the ancient DNA is on the way, however, the patterns of genetic relationships of modern Shaanxi Han, allele frequency distributions of high mutated short tandem repeats (STRs) and corresponding forensic parameters are remained to be explored.MethodsHere, we successfully genotyped 23 autosomal STRs in 630 unrelated Shaanxi male Han individuals using the recently updated Huaxia Platinum PCR amplification system. Forensic allele frequency and parameters of all autosomal STRs were assessed. And comprehensive population genetic structure was explored via various typical statistical technologies.ResultsPopulation genetic analysis based on the raw‐genotype dataset among 15,803 Eurasian individuals and frequency datasets among 56 populations generally illustrated that linguistic stratification is significantly associated with the genetic substructure of the East Asian population. Principal component analysis, multidimensional scaling plots and phylogenetic tree further demonstrated that Shaanxi Han has a close genetic relationship with geographically close Shanxi Han, and showed that Han Chinese is a homogeneous population during the historic and recent admixture from the STR variations. Except for Sinitic‐speaking populations, Shaanxi Han harbored more alleles sharing with Tibeto‐Burman‐speaking populations than with other reference populations. Focused on the allele frequency correlation and forensic parameters, all loci are in accordance with the minimum requirements of HWE and LD. The observed combined probability of discrimination of 8.2201E‐28 and the cumulative power of exclusion of 0.9999999995 in Shaanxi Han demonstrated that the studied STR loci are informative and polymorphic, and this system can be used as a powerful routine forensic tool in personal identification and parentage testing.ConclusionBoth the geographical and linguistic divisions have shaped the genetic structure of modern East Asian. And more forensic reference data should be obtained for ethnically, culturally, geographically and linguistically different populations for better routine forensic practice and population genetic studies.
Inkjet‐Based Technology for Microencapsulation of Gold Nanoparticles within Biocompatible Hydrogels
Authors: Artiga Álvaro , Ramos‐Sánchez Francisco , Serrano‐Sevilla Inés , De Matteis Laura , Mitchell Scott G. , Sánchez‐Somolinos Carlos , de la Fuente Jesús M. 
Published: March 2020

AbstractHerein, an inkjet‐based technology as a versatile high throughput methodology for the microencapsulation of gold nanoparticles (AuNPs) inside a biocompatible chitosan hydrogel is described. This continuous automated inkjet production approach generates 30 µm diameter polymeric microcapsules and offers a high rate of production and nanoparticle encapsulation efficiency of 14 nm diameter AuNPs, precise control of the microcapsule size, and ease of scale‐up. The hybrid microcapsules demonstrate biocompatible cell‐adhesion properties and resist degradation over a large range of pH, making them particularly relevant for a variety of potential health applications.
Screening for chronic prostatitis pathogens using high‐throughput next‐generation sequencing
Authors: Wu Yi , Jiang Haiyang , Tan Mingbo , Lu Xuedong 
Published: March 2020

AbstractBackgroundThe pathogens responsible for chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS; NIH category III) are not currently known. the present study utilized high‐throughput next‐generation sequencing to screen for potential pathogens associated with NIH category III CP (CP III).MethodsThis study included 33 patients with CP III and 30 healthy men, from which one sample each of urethral secretions and expressed prostatic secretion (EPS) was collected. High‐throughput next‐generation sequencing was performed to detect the sequence variations and the relative abundance of the bacterial 16S ribosomal variable region and fungal internal transcribed spacer region in all samples. Bioinformatics software and databases were used for data analysis, and differences with P < .05 were considered statistically significant.ResultsUnweighted pair group method with arithmetic mean (UPGMA) cluster analysis, principal component analysis (PCA), and Spearman's rank correlation showed that the microbial compositions of the urethral secretions and EPS collected from the same subject were essentially the same.ConclusionsNo potential pathogens were identified in diagnosed patients with CP III. The EPS may be free from bacteria before and after infection. Changes in the urinary tract microbiome may disrupt the microecological balance of the urinary system, thereby leading to CP III. Conversely, the true pathogens of CP III may not be prokaryotic or eukaryotic microorganisms, Future research may involve the evaluation of noncellular microbes.
Proteomic and transcriptomic profiling of Pten gene‐knockout mouse model of prostate cancer
Authors: Zhang Jinhui , Kim Sangyub , Li Li , Kemp Christopher J. , Jiang Cheng , Lü Junxuan 
Published: March 2020

AbstractBackgroundThe prostate‐specific phosphatase and tensin homolog deleted on chromosome 10 (Pten) gene‐conditional knockout (KO) mouse carcinogenesis model is highly desirable for studies of prostate cancer biology and chemoprevention due to its close resemblance of primary molecular defect and many histopathological features of human prostate cancer including androgen response and disease progression from prostatic intraepithelial neoplasia to invasive adenocarcinoma. Here, we profiled the proteome and transcriptome of the Pten‐KO mouse prostate tumors for global macromolecular expression alterations for signaling changes and biomarker signatures.MethodsFor proteomics, four pairs of whole prostates from tissue‐specific conditional knockout Pten‐KO mice (12‐15 weeks of age) and their respective wild‐type littermates housed in the same cages were analyzed by 8‐plex isobaric tags for relative and absolute quantitation iTRAQ. For microarray transcriptomic analysis, three additional matched pairs of prostate/tumor specimens from respective mice at 20 to 22 weeks of age were used. Real‐time quantitative reverse transcription‐polymerase chain reaction was used to verify the trends of protein and RNA expression changes. Gene Set Enrichment Analysis and Ingenuity Pathway Analysis were carried out for bioinformatic characterizations of pathways and networks.ResultsAt the macromolecular level, proteomic and transcriptomic analyses complement and cross‐validate to reveal overexpression signatures including inflammation and immune alterations, in particular, neutrophil/myeloid lineage suppressor cell features, chromatin/histones, ion and nutrient transporters, and select glutathione peroxidases and transferases in Pten‐KO prostate tumors. Suppressed expression patterns in the Pten‐KO prostate tumors included glandular differentiation such as secretory proteins and androgen receptor targets, smooth muscle features, and endoplasmic reticulum stress proteins. Bioinformatic analyses identified immune and inflammation responses as the most profound macromolecular landscape changes, and the predicted key nodal activities through Akt, nuclear factor‐kappaB, and P53 in the Pten‐KO prostate tumor. Comparison with other genetically modified mouse prostate carcinogenesis models revealed notable molecular distinctions, especially the dominance of immune and inflammation features in the Pten‐KO prostate tumors.ConclusionsOur work identified prominent macromolecular signatures and key nodal molecules that help to illuminate the patho‐ and immunobiology of Pten‐loss driven prostate cancer and can facilitate the choice of biomarkers for chemoprevention and interception studies in this clinically relevant mouse prostate cancer model.
A collaborative robotic uterine positioning system for laparoscopic hysterectomy: Design and experiments
Authors: Yip Hiu Man , Wang Zerui , Navarro‐Alarcon David , Li Peng , Cheung Tak Hong , Greiffenhagen Christian , Liu Yun‐hui 
Published: March 2020

AbstractBackgroundUterus manipulation is a lengthy and tedious task that is usually performed by a human assistant during laparoscopic hysterectomy. Note that the performance of the assistant may decrease with time. Moreover, under this approach, the primary surgeon does not have direct control over the uterus position. He/she can only verbally request the assistant to place it on a particular configuration.MethodsA robotic system composed of a 3 degrees‐of‐freedom uterine positioner is developed to assist in changing configuration of the uterus during laparoscopic hysterectomy. The developed system has a remote centre of motion structure; independently controlling the uterus motion with one joint at the time is allowed.ResultsFrom the lab experiments, it is found that the robot shows better performance in retaining the uterus position and shows quicker response to the surgeon's instruction. Cadaver studies have been conducted to evaluate the feasibility of the robot. The robot was also applied to real patients in a clinical study.ConclusionsThe robot is capable of assisting in uterus manipulation during laparoscopic hysterectomy. However, its user friendliness can be improved by simplifying the docking procedure. Furthermore, a more ergonomic user interface is desired.
Endothelial cells derived from patients’ induced pluripotent stem cells for sustained factor VIII delivery and the treatment of hemophilia A
Authors: Rose Melanie , Gao Kewa , Cortez-Toledo Elizabeth , Agu Emmanuel , Hyllen Alicia A. , Conroy Kelsey , Pan Guangjin , Nolta Jan A. , Wang Aijun , Zhou Ping 
Published: March 2020

Abstract
Hemophilia A (HA) is a bleeding disorder characterized by spontaneous and prolonged hemorrhage. The disease is caused by mutations in the coagulation factor 8 gene (F8) leading to factor VIII (FVIII) deficiency. Since FVIII is primarily produced in endothelial cells (ECs) in a non-diseased human being, ECs hold great potential for development as a cell therapy for HA. We showed that HA patient-specific induced pluripotent stem cells (HA-iPSCs) could provide a renewable supply of ECs. The HA-iPSC-derived ECs were transduced with lentiviral vectors to stably express the functional B domain deleted F8 gene, the luciferase gene, and the enhanced green fluorescent protein gene (GFP). When transplanted intramuscularly into neonatal and adult immune deficient mice, the HA-iPSC-derived ECs were retained in the animals for at least 10-16 weeks and maintained their expression of FVIII, GFP, and the endothelial marker CD31, as demonstrated by bioluminescence imaging and immunostaining, respectively. When transplanted into HA mice, these transduced HA-iPSC-derived ECs significantly reduced blood loss in a tail-clip bleeding test and produced therapeutic plasma levels (11.2%-369.2%) of FVIII. Thus, our studies provide proof-of-concept that HA-iPSC-derived ECs can serve as a factory to deliver FVIII for the treatment of HA not only in adults but also in newborns.

Significance statement The present study demonstrated that induced pluripotent stem cells (iPSCs) derived from hemophilia A (HA) patients can provide an ample supply of endothelial cells (ECs). The HA-iPSC-derived ECs can be genetically modified to produce functional factor VIII. The relatively stable engraftment of these ECs, in both neonatal and adult animals, and the functional correction or alleviation of hemophilia by these ECs in animal models, as shown in the study, provide the basis for potential therapeutic development of HA-iPSC-derived ECs for treating HA. The current study is a significant step forward in development of autologous gene-modified cell therapy for HA.
Understanding the Mechanism of SN2′ vs. SN2 in Cascade Reaction of β‐Naphthol and Nitrostyrene Derived MBH Acetates
Authors: Kumar Vadiga Shanthi , Gudise Veera Babu , Settipalli Poorna Chandrasekhar , Reddy Eeda Koti , Firoj Basha Shaik , Reddy Yeruva Pavankumar , Srinivasadesikan V. , Lee Shyi‐Long , Anwar Shaik 
Published: March 2020

AbstractThe reaction of β‐naphthol with nitrostyrene derived primary MBH acetates in presence of Cs2CO3 as base resulted in the formation of 3‐nitro‐4‐phenyl‐3,4‐dihydro‐2H‐naphthopyran as the major isomer via SN2′ process. Due to the bis‐electrophilic nature of MBH acetates, the minor product 3‐nitro‐2‐phenyl‐3,4‐dihydro‐2H‐naphthopyran was also obtained presumably due to SN2 process with
‐attack on MBH acetates. The state of the art density functional theory (DFT) calculations were carried out to account for these competitive pathways towards the formation of major and minor products.
Microwave‐Assisted Synthesis of Positional Isomeric Dihydro‐triazolo‐pyrimido‐acridines and Biological studies
Authors: Sompalle Rajesh , Roopan Selvaraj Mohana , Priya Duraipandi Devi , Suthindhiran Krishnamurthy , Sarkar Gargi , Ranjith Malavika , Arunachalapandi Murugan 
Published: March 2020

AbstractMicrowave synthetic methodologies were proposed for the preparation of positional isomeric dihydro‐triazolo‐pyrimido‐acridines, 3 a‐j and 9 a‐h via friedlander synthesis using response surface methodology (RSM). The advantages of these methodologies over conventional methods are it provides good yields with short reaction times. Free radical scavenging and antifungal studies were performed for compounds 3 a‐j and 9 a‐h. The results of antioxidant assay revealed that compounds 3 c, 3 d and 9 b exhibited excellent property whereas compounds 3 a and 9 a showed significant antifungal property against Aspergillus flavus (A.flavus) and Aspergillus niger (A. niger).
DFT Study on Oxidative Cyclization of o‐Alkynylbenzoates Mediated by Hypervalent Iodine Reagent: Mechanism and Substituent Effect
Authors: Zhou Jianguo , Song Xin‐Xin , Zhao Cheng‐Bin , Wang Ling‐Ling , Zhang Xiao‐Qian , Chen Yu , Zhang Lei 
Published: March 2020

AbstractThe reaction mechanisms of PhICl2‐mediated oxidative cyclization were characterized by density functional calculations with the m06 method, in order to unveil the plausible reaction pathway and the substituent effect on the chemoselectivity observed. The reaction of methyl o‐alkynylbenzoate 1 a and PhICl2 was used as a model, and the proposed pathway leading to the cyclization product involves the elementary steps of electrophilic attack, cyclization, intramolecular I/Cl exchange, and nucleophilic substitution, with the rate‐limiting activation barrier of 25.6 kcal/mol. The competing mechanisms of the p‐OMe‐substituted substrate 1 b and PhICl2 were comparatively characterized, and the exclusive formation of dichlorinated products rather than cyclization product should be a result of the kinetic discrimination. The free‐energy barriers of the dichlorination were computed to be 1–4 kcal/mol lower than those of the cyclization. The substituent effect was probed on the basis of geometric parameters and molecular orbitals. It was proposed that the electron‐donating property of the p‐OMe group could stabilize the alkenyl cationic intermediate, which serves a branch point of several competing pathways.
Highly‐Anisotropic Dion–Jacobson Hybrid Perovskite by Tailoring Diamine into CsPbBr3 for Polarization‐Sensitive Photodetection
Authors: Yang Tao , Li Yaobin , Han Shiguo , Xu Zhiyun , Liu Yi , Zhang Xinyuan , Liu Xitao , Teng Bing , Luo Junhua , Sun Zhihua 
Published: March 2020

Abstract2D materials with inherent attributes of structural anisotropy have been well applied in the field of polarization‐sensitive photodetection. However, to explore new 2D members with strong polarized‐light responses still remains a challenge. Herein, by alloying diamine molecule into the 3D prototype of CsPbBr3, a new Dion–Jacobson (DJ) type 2D perovskite of (HDA)CsPb2Br7 (1, where HDA2+ is 1,6‐hexamethylenediammonium), containing both inorganic Cs metal and organic cations is designed. The natural anisotropy characteristics of 1 are solidly elucidated by analyzing crystal structure, electric conductivity, and optical properties. Strikingly, distinct polarization‐sensitive responses are observed in 1, owing to its strong anisotropy of optical absorption (the ratio of αc/αb ≈ 2.2). Consequently, crystal‐based detectors of 1 exhibit fascinating photo‐activities to polarized‐light, including high detectivity (1.5 × 109 Jones), large dichroism ratio (Iphc/Iphb ≈ 1.6) and fast responding rate (200 µs). All these polarization‐sensitive performances along with intriguing phase stability make 1 a potential candidate for polarized‐light detection. This work paves a pathway toward new functionalities of DJ‐type 2D hybrid perovskites for their future optoelectronic device applications.