bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Effect of a Newly Synthesized Ferrocene Derivative against MCF-7 Breast Cancer Cells and Spheroid Stem Cells through ROS Production and Inhibition of JAK2/STAT3 Signaling Pathway
Authors: Nourbakhsh Mitra , Farzaneh Shabnam , Taghikhani Adeleh , Zarghi Afshin , Noori Shokoofe 
Published: January 2020

Background:
Breast Cancer Stem Cells (BCSCs) possess the ability of self-renewal and cellular
heterogeneity, and therefore, play a key role in the initiation, propagation and clinical outcome of breast cancer.
It has been shown that ferrocene complexes have remarkable potential as anticancer drugs.

Objective:
The present study was conducted to investigate the effects of a novel ferrocene complex, 1-
ferrocenyl-3-(4-methylsulfonylphenyl)propen-1-one (FMSP) on MCF-7 breast cancer cell line and its derived
mammospheres with cancer stem cell properties.

Methods:
Mammospheres were developed from MCF-7 cells and validated by the evaluation of CD44 and
CD24 cell surface markers by flow cytometry as well as of the expression of genes that are associated with stem
cell properties by real-time PCR. Cells viability was assessed by a soluble tetrazolium salt (MTS) after the
treatment of cells with various concentrations of FMSP. Apoptosis was evaluated by flow cytometry analysis of
annexin V and PI labeling of cells. Reactive Oxygen Species (ROS) production was measured using a cellpermeable,
oxidant-sensitive fluorescence probe (carboxy-H2DCFDA). The involvement of the JAK2/STAT3
pathway was also investigated by western blotting.

Results:
FMSP could successfully prevent mammosphere formation from differentiated MCF-7 cells and significantly
down-regulated the expression of genes involved in the production of the stem cell properties including
Wnt1, Notch1, β -catenin, SOX2, CXCR4 and ALDH1A1. FMSP decreased cell viability in both MCF-7
cells and spheroid cells, although MCF-10A cells were unaffected by this compound. Apoptosis was also dramatically
induced by FMSP, via ROS production but independent of CD95 activation. Phosphorylation levels of
JAK2 and STAT3 were also found to be significantly attenuated even in the presence of IL-6, the putative activator
of the JAK/STAT pathway.

Conclusion:
FMSP can effectively target BCSCs via ROS production and modulation of major signaling pathways
that contribute to the stemness of breast cancer cells, and therefore, might be considered a promising anticancer
agent after in vivo studies.
Analysis of Bending Ability of Soft Pneu-nets Actuators for Soft Robotics
Authors: Wang Yazhou 
Published: January 2020

Background:
There has been an increasing interest in the soft pneumatic networks (also
referred to as pneu-nets) actuators for soft robotics due to their innate softness, ease of fabrication
and high customizability. More and more structures of the soft pneu-nets actuators are reported in
various relevant patents and papers. The bending ability of soft pneu-nets actuator is one of the key
characterizing performances. It is characterized as a function of input air pressure as well as geometrical
and material parameters, and influenced by the air pressures and design angle.

Objective:
In this paper, a new structure soft pneu-nets actuators (with different chambers morphology)
was developed. The goal of this paper is to analyze the influence of the air pressures and design
angle on the bending ability of the soft pneu-nets actuators with a new structure.

Method:
Firstly, a new structure of soft pneu-nets (adjusting chamber’s shape), based on the soft
pneu-nets architecture described previously was developed. Then, the soft pneu-nets actuators were
treated with a standard Finite Element Method (FEM) using the Abaqus 6.14 Computer-Aided Engineering
(CAE) package. Several soft pneu-nets actuators with various design angles were analyzed
to investigate the influence of the design angle on the bending ability. In order to investigate
the effect of these parameters, the relationship between the angle of bending and these parameters
was conducted. Thirdly, the influence of chambers morphology on the bending ability of soft pneunets
actuators could be assessed.

Results:
When the air pressure P is under 13.5 kPa, with increase the air pressure, the difference of
angle of bending under same design angle is less evident, but when the air pressure P is over 13.5
kPa, the difference of angle of bending at same design angle increase; At the same air pressure P,
when the air pressure P is under 13.5 kPa, the difference of the angle of the bending between different
design angle is less, The effect of gravity is greater than that of the air pressures P. When the air
pressure P is over 13.5 kPa, the design angle shows more influential on the angle of the bending.

Conclusion:
The angle of bending increases with the increase of the air pressures P; the chambers
with a bigger design angle (thinner inside walls) enabled greater bending at given air pressures P.
EFFECTS OF EXTRA VIRGIN OLIVE OIL VERSUS RICE BRAN OIL ON GLYCEMIC CONTROL IN PATIENTS WITH TYPE-2 DIABETES MELLITUS
Authors: WIJAYANTHIE NADIYAH , GUNARTI DWIRINI RETNO , MANIKAM NURUL RATNA , YULHASRI  
Published: January 2020

Objective: The purpose of this study was to determine the effect of extra virgin olive oil (EVOO) and rice bran oil (RBO) on glycemic control and lipid profiles in patients with type-2 diabetes mellitus (T2DM).
Methods: Ten patients with T2DM received 15 ml/day of EVOO or RBO. Levels of fasting blood glucose (FBG), postprandial blood glucose (PBG), total cholesterol (TC), low-density lipoprotein-cholesterol (LDL-C), high-density lipoprotein-cholesterol (HDL-C), and triglycerides (TGs) were measured. RBO or EVOO was administered for 4 consecutive weeks. During a 2-week interval, the treatment was not administered. After this washout period, a crossover design was implemented by exchanging EVOO supplementation with RBO supplementation and vice versa for 4 consecutive weeks.
Results: Changes in levels of FBG, PBG, TC, LDL-C, and TGs were not significantly different in the two groups. However, significantly decreased the levels of HDL-C were observed in both groups.
Conclusion: RBO and EVOO had no significant influence on levels of FBG or PBG.
THE ROLE OF TREATMENT WITH HIGH-DOSE CORTICOSTEROIDS IN SEVERE LEPTOSPIROSIS WITH PULMONARY COMPLICATIONS
Authors: PARAMITHA MARIA SATYA , SUTRISUNA  , PUSPITASARI MYRA 
Published: January 2020

Objective: To investigate the effectiveness of high-dose corticosteroids in patients with severe leptospirosis, especially in reducing morbidity and mortality rates.
Methods: Literature searches were conducted using PubMed® and SCOPUS®. One systematic review and one systematic review with meta-analysis were selected from three chosen studies.
Results: The systematic review from Rodrigo et al. only included qualitative synthesis from four interventional studies. Early administration of methylprednisolone can be beneficial; however, low number and poor methodological quality of the studies question the validity of this review. Duggal et al. stated that there was no significant difference in the therapeutic effects of early high-dose corticosteroids (P ³ 0.05; 95% CI: 0.81–1.37), early low-dose corticosteroids (P ³ 0.05; 95% CI: 0.3–1.03), and late low-dose corticosteroids (P ³ 0.05; 95% CI: 0.11–2.52). Limited number of studies and high statistical heterogeneity, however, are major drawbacks for this review.
Conclusion: Evidence about the role of high-dose corticosteroids in severe leptospirosis patients with pulmonary complications, especially their benefits, is still limited. Additional randomized, controlled trials with adequate statistical power still need to be conducted.
THE EFFECT OF LUNASIN ON LIVER HISTOPATHOLOGY OF MICE INDUCED BY AZOXIMETHANE (AOM) AND DEXTRAN SODIUM SULPHATE (DSS)
Authors: KUSMARDI1  , KHIPUTRA HERIYANTO , SALINAH  
Published: January 2020

Objective: Azoximethane (AOM) and dextran sodium sulfate (DSS) are commonly used to induce colorectal cancer, but have side effects on the liver. AOM can induce hepatic histopathological changes such as the appearance of microvesicular steatosis and necrosis. DSS plays a role in causing inflammation and indirect scarring through colitis. This study aimed to determine in mice the effect of lunasin on the liver histopathology induced by AOM and DSS.
Methods: This was an experimental study conducted in BALB/c mice. The data analyzed were the results of quantification of foci of necrosis, steatosis, and dysplasia from liver preparations of mice given lunasin extract at doses of 20, 30, and 40 mg/kg and control group.
Results: This study showed a significant decrease in the number of foci of necrosis in the group given 30 mg/kg lunasin, with a mean score of 9.0±3.4 compared with the control group mean score of 14.0±0.8 (P = 0.017). There was also a significant decrease in the number of foci of steatosis in the group given 30 mg/kg lunasin with a mean score of 3.8±1.3 compared with the control group mean score of 11.5±1.9 (P = 0.002).
Conclusion: The results of this study indicate that lunasin at doses above 30 mg/kg can inhibit the formation of foci of necrosis and steatosis induced in mouse liver by AOM and DSS.
QUERCETIN IMPROVES THE EFFICACY OF SORAFENIB IN TRIPLE NEGATIVE BREAST CANCER CELLS THROUGH THE MODULATION OF DRUG EFFLUX TRANSPORTERS EXPRESSIONS
Authors: LOUISA MELVA , WARDHANI BANTARI WK 
Published: January 2020

Objective: This study aimed to investigate whether quercetin is able to improve the efficacy of sorafenib in triple negative breast cancer cells and explore the possibility of drug efflux transporters modulation by quercetin.
Methods: We exposed MDA-MB-231, a triple negative breast cancer cell line, to several groups: sorafenib alone, quercetin alone, a combination of sorafenib-quercetin, and control. We determined cell viability over control weekly up to 4 w. At the end of the fourth week, mRNA expressions of drug efflux transporters (P-glycoprotein and breast cancer resistance protein [BCRP] and MRP2 [multidrug resistance-associated protein-2]) were examined.
Results: Sorafenib alone was shown to maintain its efficacy for only two weeks, while quercetin alone was able to maintain its effect for four weeks. A combination of sorafenib-quercetin showed the best cytotoxicity effects compared with sorafenib or quercetin alone and was able to maintain its efficacy for four weeks. There were increased mRNA expressions of P-glycoprotein, BCRP, and MRP2 after four weeks of treatment with sorafenib, while treatment with quercetin decreased the drug efflux transporters expressions. A combination of sorafenib-quercetin decreased the mRNA expressions of both P-glycoprotein and BCRP, compared with sorafenib alone.
Conclusion: We suggest that decreased expressions of both drug efflux transporters, P-glycoprotein and BCRP, mediated by quercetin ameliorate the efficacy of sorafenib in TNBC. Therefore, the addition of quercetin to sorafenib might be useful in the future in improving the therapeutic efficacy of sorafenib in triple negative breast cancer.
THE EFFECTS OF CURCUMIN ADMINISTRATION ON EXPRESSION PATTERNS OF VEGF AND COX-2 IN FERTILE ENDOMETRIUM: A RANDOMISED CLINICAL TRIAL
Authors: RAJUDDIN RAJUDDIN , WIWEKO BUDI , NUGROHO LUTFI 
Published: January 2020

Objective: Curcumin (diferuloylmethane) is a a compound isolated from turmeric with biological activities, including antifertility. Curcumin inhibits COX-2 expression in granulosa cells of ovarian follicles and disrupts vascular endothelial growth factor (VEGF) derived angiogenesis in the endometrium, reducing endometrial receptivity. The purpose of this study was to examine the effects of curcumin on COX-2 and VEGF expression in endometrium of fertile women.
Methods: A prospective double-blind placebo-controlled clinical trial was conducted in a group of fertile women with regular menstrual cycles, aged between 20-30 y, married, and with children. Subjects were divided into a group receiving daily 800 mg encapsulated curcumin. Curcumin orally for ten days, starting on the third day of the first menstrual day, and a control group. Endometrial biopsy was performed using a microcuret and immunohistochemistry was used to assess VEGF and COX-2 expression. The results were analysed using an independent sample t-test.
Results: In the curcumin-treated group, VEGF expression was significantly lower than the control group (p<0.05), and COX-2 expression was higher but not significantly so (p>0.05).
Conclusion: The curcumin causes VEGF expression in endometrium is lower and negatively affects the growth of endometrial stromal cells.
ZINC OXIDE NANOPARTICLES AMELIORATE ALUMINUM CHLORIDE-INDUCED HEPATO-RENAL OXIDATIVE STRESS AND INFLAMMATION IN RATS
Authors: MAHMOUD SAHAR M. , KASSAB RAMI B. , MONEIM AHMED E. ABDEL 
Published: January 2020

Objective: The present study was designed to evaluate the effect of zinc oxide nanoparticles (ZnO NPs) on Aluminum chloride (AlCl3)-induced hepato-renal injury.
Methods: Animals were divided into, I-control group; rats received saline, II-AlCl3 group; animals received 100 mg AlCl3/kg body weight, III-ZnO NPs group; rats received 10 mg ZnO NPs/kg body weight, and IV group ZnO NPs+AlCl3. All rats were administered their respective doses daily for 6 w. Hepatorenal function parameters in sera; aminotransferases, bilirubin, urea, and creatinine were estimated. Lipid peroxide level and nitrite\nitrate ratio, glutathione content, glutathione peroxidase, glutathione reductase, catalase, superoxide dismutase activities and interleukin-1β, tumor necrosis factor-α levels were determined in both tissues. The histopathological and the immunohistochemical investigations of nuclear factor-kB expression were carried out.
Results: ZnO NPs treatment to AlCl3-intoxicated rats significantly reduced Al accumulation (at p<0.05) in the hepatorenal tissue and increased zinc accumulation (at p<0.05) in liver and kidney, respectively, with respect to AlCl3-group, thus inhibiting oxidative stress and inflammation parameters represented by lipid peroxidation and nitric oxide levels (at p<0.05) compared to AlCl3 group and elevated antioxidant parameters (at p<0.05), compared to AlCl3 treated group, while suppressed interleukin-1β, tumor necrosis factor-α levels (at p<0.05) and the nuclear factor-kB activation in liver and kidney, especially in the kidney if compared to AlCl3-treated group. Hepatorenal function indices indicated significant decreases compared to AlCl3 group (at p<0.05).
Conclusion: Results indicated the ameliorative effect of ZnO NPs on aluminum-induced hepato-renal damage.
OPTIMIZATION OF ROLL COMPACTOR VARIABLES AND FORMULATION OF ANTI-RETROVIRAL TABLET BY ROLL COMPACTION METHOD
Authors: LAHANE SUHAS H. 
Published: January 2020

Objective: This study emphasis on roll compaction variable and how the processing parameters influence the formation of granules in process of formulations of antiretroviral IR Tablet with help of optimization technique.
Methods: In this present work we aimed to develop a stable pharmaceutical dosage form with anti-retroviral drug tenofovir disoproxil fumarate. % retention of granules over # 60 mesh in roll compaction method by sizing with 50G co-mill screen was assessed by optimization and results were evaluated by Design expert 12.0 software. Various parameters and optimization of the parameter for formulation for better product was done by using 23 factorial design and dry granulation technique for manufacturing tablets. Three operating parameters the roller speed, the hydraulic pressure and the gap width on the Chamunda CPMRC-200/150 Roll Compactor were varied. The planned response variable for study was % retention over #60 ASTM mesh. % retention of granules was calculated by weighing granules on digital electronic balance with respect to how much premix material was taken for compaction.
Results: Excipients compatibility study gave positive way showing no change in physical appearance of drug-excipients mix. It reviled that drug was compatible with excipients used.
By formation of granules with required ratio, the value of Compressibility index changed from 29 to 21.89, showed that flow properties were improved i.e. from poor to passable.
Design expert 12.0 gave optimized solution for formation of required quantity of granules. Pareto chart showed envaulted positive and negative impact of factors on response as explained in results.
The results clearly indicate that how granules manufacturing in roll compaction process are influenced by roller pressure, roller gap and speed. 70 % flakes formation and granules retention were observed with 4000 kg/cm2 pressure, 1 mm roller gap width and 6 rpm speed of roller.
Pareto chart clearly indicate major impact is of roller pressure. Comparative dissolution profile graph showed that drug release pattern is similar with the innovator tablet.
A stable, robust tablets were formed at the end of process.
Conclusion: In this study, by optimizing processing variables stable antiretroviral immediate release oral solid dosage form was formed.
OPTIMALISASI LIMBAH AGROINDUSTRI SEBAGAI UPAYA MENINGKATKAN PEREKONOMIAN MASYARAKAT DI DESA BANYURESMI KABUPATEN SUMEDANG
Authors: Putri Sherly Ayuna , Hidayah Rohim , Rismayanti Resa , Apriliani Anggun , Putri Kamal Salwa Alifiah 
Published: January 2020

Kegiatan pengabdian masyarakat di Desa Banyuresmi dilakukan dengan observasi lapangan mengenai permasalahan limbah agroindustri. Mayoritas masyarakat disana menghasilkan limbah dari kegiatan pengelolaan pascapanen kopi dan tembakau. Sampai saat ini di Desa Banyuresmi masih terbatas dalam mengelola limbah agroindustri berupa kulit kopi dan tembakau. Disisi lain, limbah tersebut dapat menjadi sebuah produk yang memiliki nilai ekonomi tinggi. BOTALIKA merupakan sebuah inovasi pembuatan boneka horta berbentuk macan dari limbah kopi dan tembakau. Produk tersebut dapat mendukung Desa Banyuresmi sebagai desa wisata Pasirmacan dengan memanfaatkan potensi lokal untuk meningkatkan perekonomian masyarakat. Proyek ini kami kembangkan setelah melihat besarnya potensi lokal dari limbah kopi dan tembakau yang terdapat di Desa Banyuresmi. Keindahan alam yang dimiliki Desa Banyuresmi sangat mendukung untuk menjadikan desa tersebut menjadi sebuah desa wisata. Nantinya, boneka horta “BOTALIKA” dapat menjadi souvenir khas Desa Banyuresmi sebagai desa wisata Pasirmacan. The social service program in Banyuresmi village was done through field observations regarding the issues of agroindustrial waste. The majority of the public there created waste from the post-harvest activities of coffee and tobacco. To this day the utilization of agroindustrial waste in Banyuresmi village is limited to coffee been skin and tobacco. On the other hand, the waste can become a product with high economical value. BOTALIKA is a new innovation that creates horta dolls in the shape of a tiger from coffee and tobacco waste. The product can support Banyuresmi village as a the Pasirmacan tourism village through the utilization of local potentials in order to increase the community’s economy. The project was developed after identifying the local potential from coffee and tobacco waste that is in Banyuresmi village. The natural beauty of Banyuresmi village bolsters its efforts to become a tourism village. With time, the BOTALIKA horta dolls can become a souvenir from Banyuresmi village as the Pasirmacan tourisme village.
O nosso espelho do Terror
Authors: Pareto Junior Lindener 
Published: January 2020

Resenha de “No espelho do terror: Jihad e espetáculo” (São Paulo: Editora Elefante, 64 pp.) de Gabriel Ferreira Zacarias. Sexta-feira, 13 de novembro de 2015: uma série orquestrada de ataques terroristas em diversospontos da cidade de Paris matou, a tiros de fuzis, centenas de pessoas. Na casa de shows Bataclan,três homens fortemente armados invadiram o recinto, fizeram reféns, declararam palavras de ordemjihadistas, mataram 89 pessoas e se suicidaram logo em seguida, numa carnificina que remontaria aosmomentos mais agudos das revoluções, guerras e ocupações que a cidade presenciou desde o séculoXVIII. Os parisienses viviam, meses depois do ataque à redação do Charlie Hebdo, uma noite de terrorcomo poucas vezes presenciaram após o fim da Segunda Guerra Mundial. Entre os brasileiros que viviamem Paris e que se marcaram “seguros” na opção da rede social Facebook, estava o autor do livro ora emquestão, Gabriel Ferreira Zacarias.
Islam dalam Tradisi Pernikahan
Authors: Nuraeni Nuraeni 
Published: January 2020

This paper examines the integration of Islam in the tradition of marriage to the people in the village of Bontosunggu. The source used is based on field research and books related to the research title. The results of the study found that some implementation of the marriage tradition in Bontosunggu, Bontonompo District, Gowa, had integrated Islamic values. Akkorongtigi, Simorong, A'nikka, Nilekka, Nilekka are a series of marriages that must be preceded by Angngalle je'ne sambayang (Ablution), reading shalawat, barazanji and appatamma (Khataman al-Qur'an) as a tradition that has precedence with Islamic values which are Islamic values, performing wudoo, reading shalawat, barazanji and appatamma (Khataman al-Qur'an) as traditions that have Islamic values preceded by Islamic values. integrated with local traditions.Artikel ini membahas integrasi Islam dalam tradisi pernikahan pada masyarakat di desa bontosunggu. Sumber yang digunakan berdasar pada penelitian lapangan observasi dan wawancara serta buku-buku yang berhubungan judul penelitian. Hasil penelitian menemukan beberapa pelaksanaan tradisi pernikahan di Bontosunggu Kecamatan Bontonompo Gowa telah terintegrasi nilai-nilai Islam. Akkorongtigi, Simorong, A’nikka, Nilekka, Nilekka adalah rangkian pelaksaaan pernikahan harus didahului dengan Angngalle je’ne sambayang (Berwudhu), membaca shalawat, barazanji dan appatamma (Khataman al-Qur’an) sebagai tradisi yang memiliki nilai-nilai Islam yang terintegrasi dengan tradisi lokal.