bluuebunny/crossref_metadata_2025_split huggingface dataset:
Quorum sensing controls persistence, resuscitation, and virulence of Legionella subpopulations in biofilms
Authors: Personnic Nicolas , Striednig Bianca , Hilbi Hubert 
Published: September 2020

Abstract
The water-borne bacterium Legionella pneumophila is the causative agent of Legionnaires’ disease. In the environment, the opportunistic pathogen colonizes different niches, including free-living protozoa and biofilms. The physiological state(s) of sessile Legionella in biofilms and their functional consequences are not well understood. Using single-cell techniques and fluorescent growth rate probes as well as promoter reporters, we show here that sessile L. pneumophila exhibits phenotypic heterogeneity and adopts growing and nongrowing (“dormant”) states in biofilms and microcolonies. Phenotypic heterogeneity is controlled by the Legionella quorum sensing (Lqs) system, the transcription factor LvbR, and the temperature. The Lqs system and LvbR determine the ratio between growing and nongrowing sessile subpopulations, as well as the frequency of growth resumption (“resuscitation”) and microcolony formation of individual bacteria. Nongrowing L. pneumophila cells are metabolically active, express virulence genes and show tolerance toward antibiotics. Therefore, these sessile nongrowers are persisters. Taken together, the Lqs system, LvbR and the temperature control the phenotypic heterogeneity of sessile L. pneumophila, and these factors regulate the formation of a distinct subpopulation of nongrowing, antibiotic tolerant, virulent persisters. Hence, the biofilm niche of L. pneumophila has a profound impact on the ecology and virulence of this opportunistic pathogen.
The effect of intranasal oxytocin on visual processing and salience of human faces
Authors: Hovey Daniel , Martens Louise , Laeng Bruno , Leknes Siri , Westberg Lars 
Published: September 2020

AbstractThe mechanisms underlying the role of oxytocin (OT) as a regulator of social behavior in mammals are only partly understood. Recently, it has been proposed that OT increases the salience of social stimuli. We carried out a randomized, double-blind, cross-over study of the effects of OT on binocular rivalry, a visual phenomenon underpinned by the interplay of excitation and inhibition in the cortex. A final sample of 45 participants viewed images of social stimuli (faces with different emotional expressions) and non-social stimuli (houses and Gabor patches). We demonstrate a robust effect that intranasal OT increases the salience of human faces in binocular rivalry, such that dominance durations of faces are longer—this effect is not modulated by the facial expression. We tentatively show that OT treatment increases dominance durations for non-social stimuli. Our results lend support to the social salience hypothesis of OT, and in addition offer provisional support for the role of OT in influencing excitation-inhibition balance in the brain.
The prevalence of psychiatric symptoms of pregnant and non-pregnant women during the COVID-19 epidemic
Authors: Zhou Yongjie , Shi Hui , Liu Zhengkui , Peng Songxu , Wang Ruoxi , Qi Ling , Li Zezhi , Yang Jiezhi , Ren Yali , Song Xiuli , Zeng Lingyun , Qian Wei , Zhang Xiangyang 
Published: September 2020

AbstractCoronavirus disease 2019 (COVID-19) is rapidly spreading worldwide, with a staggering number of cases and deaths. However, available data on the psychological impacts of COVID-19 on pregnant women are limited. The purposes of this study were to assess the prevalence of psychiatric symptoms among pregnant women, and to compare them with non-pregnant women. From February 28 to March 12, 2020, a cross-sectional study of pregnant and non-pregnant women was performed in China. The online questionnaire was used to collect information of participants. The mental health status was assessed by patient health questionnaire, generalized anxiety disorder scale, insomnia severity index, somatization subscale of the symptom checklist 90, and post-traumatic stress disorder (PTSD) checklist-5. Totally, 859 respondents were enrolled, including 544 pregnant women and 315 non-pregnant women. In this study, 5.3%, 6.8%, 2.4%, 2.6%, and 0.9% of pregnant women were identified to have symptoms of depression, anxiety, physical discomfort, insomnia, and PTSD, respectively. However, the corresponding prevalence rates among non-pregnant women were 17.5%, 17.5%, 2.5%, 5.4%, 5.7%, respectively. After adjusting for other covariates, we observed that pregnancy was associated a reduced risk of symptoms of depression (OR = 0.23; 95% CI: 0.12–0.45), anxiety (OR = 0.26; 95% CI: 0.16–0.42), insomnia (OR = 0.19; 95% CI: 0.06–0.58), and PTSD (OR = 0.15; 95% CI: 0.04–0.53) during the COVID-19 epidemic. Our results indicate that during the COVID-19 epidemic in China, pregnant women have an advantage of facing mental problems caused by COVID-19, showing fewer depression, anxiety, insomnia, and PTSD symptoms than non-pregnant women.
HOTAIR contributes to the carcinogenesis of gastric cancer via modulating cellular and exosomal miRNAs level
Authors: Zhang Jie , Qiu Wei-qing , Zhu Hongyi , Liu Hua , Sun Jian-hua , Chen Yuanwen , Shen Huojian , Qian Chang-lin , Shen Zhi-yong 
Published: September 2020

AbstractGastric cancer (GC) is one of the most leading malignancies. Long noncoding RNA is related to GC. In this study, 11 miRNAs in the exosomes and six lncRNAs in the tissues was examined by qRT-PCR. Correlation analysis was used to analyze the relationship between miRNAs in exosome and lncRNAs in the tissues. Four miRNAs level in GC tissues were examined by qRT-PCR. MTT was used to determine cell viability. Flow cytometry was used to quantify the apoptotic cells. Transwell assay was used to examine the migration and invasion capacity. Dual-luciferase assay was used to examine the interaction between HOTAIR and miR-30a or -b. Capillary formation was used to determine the capillary formation capacity. Weak negative correlations were found between HOTAIR and miR-30a or -b in GC tissue samples. Interestingly, strong negative correlations were identified between the HOTAIR level in GC tissue samples and the miR-30a or -b levels in plasma exosomes. HOTAIR knockdown GC cells exhibited decreased migration, invasion, proliferation, and upregulated apoptosis, which released more miR-30a and -b into the exosomes. KRAS was upregulated when co-cultured with exosomes from HOTAIR overexpressed cells, and promoted GC cells proliferation, migration, and invasion. Meanwhile, HUVEC cells expressed increased VEGF-A and formatted more capillaries. Subsequently, we identified a 10mer target site of miR-30a or -b in HOTAIR sequence, and the overexpression of HOTAIR induced the degradation of miR-30a or -b, indicating a ceRNA role of HOTAIR. We report the negative correlation between the plasma miRNAs level and GC tissue HOTAIR expression for the first time and unveiled the ceRNA role of HOTAIR in GC. HOTAIR functions as an onco-lncRNA regulating the level of miR-30a and -b in both GC cells and exosomes. These findings may give insight into understanding the mechanism of GC pathogenesis and provide new biomarkers for clinical diagnosis.
Optical gap and fundamental gap of oligoynes and carbyne
Authors: Zirzlmeier Johannes , Schrettl Stephen , Brauer Jan C. , Contal Emmanuel , Vannay Laurent , Brémond Éric , Jahnke Eike , Guldi Dirk M. , Corminboeuf Clémence , Tykwinski Rik R. , Frauenrath Holger 
Published: September 2020

AbstractThe optoelectronic properties of various carbon allotropes and nanomaterials have been well established, while the purely sp-hybridized carbyne remains synthetically inaccessible. Its properties have therefore frequently been extrapolated from those of defined oligomers. Most analyses have, however, focused on the main optical transitions in UV-Vis spectroscopy, neglecting the frequently observed weaker optical bands at significantly lower energies. Here, we report a systematic photophysical analysis as well as computations on two homologous series of oligoynes that allow us to elucidate the nature of these weaker transitions and the intrinsic photophysical properties of oligoynes. Based on these results, we reassess the estimates for both the optical and fundamental gap of carbyne to below 1.6 eV, significantly lower than previously suggested by experimental studies of oligoynes.
Risk prediction of late-onset Alzheimer’s disease implies an oligogenic architecture
Authors: Zhang Qian , Sidorenko Julia , Couvy-Duchesne Baptiste , Marioni Riccardo E. , Wright Margaret J. , Goate Alison M. , Marcora Edoardo , Huang Kuan-lin , Porter Tenielle , Laws Simon M. ,   Australian Imaging Biomarkers and Lifestyle (AIBL) Study, Masters Colin L. , Bush Ashley I. , Fowler Christopher , Darby David , Pertile Kelly , Restrepo Carolina , Roberts Blaine , Robertson Jo , Rumble Rebecca , Ryan Tim , Collins Steven , Thai Christine , Trounson Brett , Lennon Kate , Li Qiao-Xin , Ugarte Fernanda Yevenes , Volitakis Irene , Vovos Michael , Williams Rob , Baker Jenalle , Russell Alyce , Peretti Madeline , Milicic Lidija , Lim Lucy , Rodrigues Mark , Taddei Kevin , Taddei Tania , Hone Eugene , Lim Florence , Fernandez Shane , Rainey-Smith Stephanie , Pedrini Steve , Martins Ralph , Doecke James , Bourgeat Pierrick , Fripp Jurgen , Gibson Simon , Leroux Hugo , Hanson David , Dore Vincent , Zhang Ping , Burnham Samantha , Rowe Christopher C. , Villemagne Victor L. , Yates Paul , Pejoska Sveltana Bozin , Jones Gareth , Ames David , Cyarto Elizabeth , Lautenschlager Nicola , Barnham Kevin , Cheng Lesley , Hill Andy , Killeen Neil , Maruff Paul , Silbert Brendan , Brown Belinda , Sohrabi Harmid , Savage Greg , Vacher Michael , Sachdev Perminder S. , Mather Karen A. , Armstrong Nicola J. , Thalamuthu Anbupalam , Brodaty Henry , Yengo Loic , Yang Jian , Wray Naomi R. , McRae Allan F. , Visscher Peter M. 
Published: September 2020

AbstractGenetic association studies have identified 44 common genome-wide significant risk loci for late-onset Alzheimer’s disease (LOAD). However, LOAD genetic architecture and prediction are unclear. Here we estimate the optimalP-threshold (Poptimal) of a genetic risk score (GRS) for prediction of LOAD in three independent datasets comprising 676 cases and 35,675 family history proxy cases. We show that the discriminative ability of GRS in LOAD prediction is maximised when selecting a small number of SNPs. Both simulation results and direct estimation indicate that the number of causal common SNPs for LOAD may be less than 100, suggesting LOAD is more oligogenic than polygenic. The best GRS explains approximately 75% of SNP-heritability, and individuals in the top decile of GRS have ten-fold increased odds when compared to those in the bottom decile. In addition, 14 variants are identified that contribute to both LOAD risk and age at onset of LOAD.
Macrophage secretion of miR-106b-5p causes renin-dependent hypertension
Authors: Oh J. , Matkovich S. J. , Riek A. E. , Bindom S. M. , Shao J. S. , Head R. D. , Barve R. A. , Sands M. S. , Carmeliet G. , Osei-Owusu P. , Knutsen R. H. , Zhang H. , Blumer K. J. , Nichols C. G. , Mecham R. P. , Baldán Á , Benitez B. A. , Sequeira-Lopez M. L. , Gomez R. A. , Bernal-Mizrachi C. 
Published: September 2020

AbstractMyeloid cells are known mediators of hypertension, but their role in initiating renin-induced hypertension has not been studied. Vitamin D deficiency causes pro-inflammatory macrophage infiltration in metabolic tissues and is linked to renin-mediated hypertension. We tested the hypothesis that impaired vitamin D signaling in macrophages causes hypertension using conditional knockout of the myeloid vitamin D receptor in mice (KODMAC). These mice develop renin-dependent hypertension due to macrophage infiltration of the vasculature and direct activation of renal juxtaglomerular (JG) cell renin production. Induction of endoplasmic reticulum stress in knockout macrophages increases miR-106b-5p secretion, which stimulates JG cell renin production via repression of transcription factors E2f1 and Pde3b. Moreover, in wild-type recipient mice of KODMAC/miR106b−/− bone marrow, knockout of miR-106b-5p prevents the hypertension and JG cell renin production induced by KODMAC macrophages, suggesting myeloid-specific, miR-106b-5p-dependent effects. These findings confirm macrophage miR-106b-5p secretion from impaired vitamin D receptor signaling causes inflammation-induced hypertension.
Conversion of anilines to chiral benzylic amines via formal one-carbon insertion into aromatic C–N bonds
Authors: Li Lei , Yang Min , He Qiuqin , Fan Renhua 
Published: September 2020

AbstractInsertion of atoms into aromatic carbon-nitrogen bonds is an appealing method for the synthesis of nitrogen-containing molecules and it has the advantage of the availability and abundance of anilines. However, the direct cleavage of aromatic carbon-nitrogen bonds is challenging due to the particularly inert and stable nature of these bonds. Here we report a formal, enantioselective one-carbon insertion into an aromatic carbon-nitrogen bond via an aromaticity dissembly-reconstruction process to directly convert anilines to chiral α-branched benzylic amines. The process involves oxidative dearomatization of para-substituted anilines, chiral sulfur ylide-mediated asymmetric aziridination, and subsequent rearrangement. Chiral sulfur ylides serve as one-carbon insertion units.
Structural characterization of a neutralizing mAb H16.001, a potent candidate for a common potency assay for various HPV16 VLPs
Authors: Huang Weijin , He Maozhou , Ning Tingting , Nie Jianhui , Zhang Feng , Zheng Qingbing , Zhang Rui , Xu Ying , Gu Ying , Li Shaowei , Wang Youchun 
Published: September 2020

AbstractWith more human papillomavirus (HPV) virus-like particle (VLP) vaccines to hit the market in future, a monoclonal antibody (mAb) with preferably comparable reactivity against vaccines from different expression systems and bioprocesses is urgently needed for the potency characterization. Among all mAbs against HPV16 collected, rabbit mAb H16.001 is potently neutralizing with the highest affinity, recognizes an immune-dominant epitope, and can comparably react with HPV16 vaccines from various sources. Cryo-electron microscopic (cryo-EM) structure demonstrated that 360 H16.001 Fabs could bind to HPV16 capsid in preferable binding manner without steric hindrance between neighboring Fabs, potentially supporting its identification for VLP structural integrity and utility in monitoring VLP structural probity. This structural analysis indicated that mAb H16.001 afforded unbiased potency characterization for various HPV16 vaccines and was potential for use in vaccine regulation practice. This study also showed a model process for selecting suitable mAbs for potency assays of other vaccines.
Hourly 5-km surface total and diffuse solar radiation in China, 2007–2018
Authors: Jiang Hou , Lu Ning , Qin Jun , Yao Ling 
Published: September 2020

AbstractSurface solar radiation is an indispensable parameter for numerical models, and the diffuse component contributes to the carbon uptake in ecosystems. We generated a 12-year (2007–2018) hourly dataset from Multi-functional Transport Satellite (MTSAT) satellite observations, including surface total solar radiation (Rs) and diffuse radiation (Rdif), with 5-km spatial resolution through deep learning techniques. The used deep network tacks the integration of spatial pattern and the simulation of complex radiation transfer by combining convolutional neural network and multi-layer perceptron. Validation against ground measurements shows the correlation coefficient, mean bias error and root mean square error are 0.94, 2.48 W/m2 and 89.75 W/m2 for hourly Rs and 0.85, 8.63 W/m2 and 66.14 W/m2 for hourly Rdif, respectively. The correlation coefficient of Rs and Rdif increases to 0.94 (0.96) and 0.89 (0.92) at daily (monthly) scales, respectively. The spatially continuous hourly maps accurately reflect regional differences and restore the diurnal cycles of solar radiation at fine resolution. This dataset can be valuable for studies on regional climate changes, terrestrial ecosystem simulations and photovoltaic applications.
Crop climate suitability mapping on the cloud: a geovisualization application for sustainable agriculture
Authors: Peter Brad G. , Messina Joseph P. , Lin Zihan , Snapp Sieglinde S. 
Published: September 2020

AbstractClimate change, food security, and environmental sustainability are pressing issues faced by today’s global population. As production demands increase and climate threatens crop productivity, agricultural research develops innovative technologies to meet these challenges. Strategies include biodiverse cropping arrangements, new crop introductions, and genetic modification of crop varieties that are resilient to climatic and environmental stressors. Geography in particular is equipped to address a critical question in this pursuit—when and where can crop system innovations be introduced? This manuscript presents a case study of the geographic scaling potential utilizing common bean, delivers an open access Google Earth Engine geovisualization application for mapping the fundamental climate niche of any crop, and discusses food security and legume biodiversity in Sub-Saharan Africa. The application is temporally agile, allowing variable growing season selections and the production of ‘living maps’ that are continually producible as new data become available. This is an essential communication tool for the future, as practitioners can evaluate the potential geographic range for newly-developed, experimental, and underrepresented crop varieties for facilitating sustainable and innovative agroecological solutions.
Prospective associations of the infant gut microbiome and microbial function with social behaviors related to autism at age 3 years
Authors: Laue Hannah E. , Korrick Susan A. , Baker Emily R. , Karagas Margaret R. , Madan Juliette C. 
Published: September 2020

AbstractThe hypothesized link between gut bacteria and autism spectrum disorder (ASD) has been explored through animal models and human studies with microbiome assessment after ASD presentation. We aimed to prospectively characterize the association between the infant/toddler gut microbiome and ASD-related social behaviors at age 3 years. As part of an ongoing birth cohort gut bacterial diversity, structure, taxa, and function at 6 weeks (n = 166), 1 year (n = 158), 2 years (n = 129), and 3 years (n = 140) were quantified with 16S rRNA gene and shotgun metagenomic sequencing (n = 101 six weeks, n = 103 one year). ASD-related social behavior was assessed at age 3 years using Social Responsiveness Scale (SRS-2) T-scores. Covariate-adjusted linear and permutation-based models were implemented. Microbiome structure at 1 year was associated with SRS-2 total T-scores (p = 0.01). Several taxa at 1, 2, and 3 years were associated with SRS-2 performance, including many in the Lachnospiraceae family. Higher relative abundance of Adlercreutzia equolifaciens and Ruminococcus torques at 1 year related to poorer SRS-2 performance. Two functional pathways, l-ornithine and vitamin B6 biosynthesis, were associated with better social skills at 3 years. Our results support potential associations between early-childhood gut microbiome and social behaviors. Future mechanistic studies are warranted to pinpoint sensitive targets for intervention.
Live-cell imaging with Aspergillus fumigatus-specific fluorescent siderophore conjugates
Authors: Pfister Joachim , Lichius Alexander , Summer Dominik , Haas Hubertus , Kanagasundaram Thines , Kopka Klaus , Decristoforo Clemens 
Published: September 2020

AbstractLive-cell imaging allows the in vivo analysis of subcellular localisation dynamics of physiological processes with high spatial–temporal resolution. However, only few fluorescent dyes have been custom-designed to facilitate species-specific live-cell imaging approaches in filamentous fungi to date. Therefore, we developed fluorescent dye conjugates based on the sophisticated iron acquisition system of Aspergillus fumigatus by chemical modification of the siderophore triacetylfusarinine C (TAFC). Various fluorophores (FITC, NBD, Ocean Blue, BODIPY 630/650, SiR, TAMRA and Cy5) were conjugated to diacetylfusarinine C (DAFC). Gallium-68 labelling enabled in vitro and in vivo characterisations. LogD, uptake assays and growth assays were performed and complemented by live-cell imaging in different Aspergillus species. Siderophore conjugates were specifically recognised by the TAFC transporter MirB and utilized as an iron source in growth assays. Fluorescence microscopy revealed uptake dynamics and differential subcellular accumulation patterns of all compounds inside fungal hyphae.[Fe]DAFC-NBD and -Ocean Blue accumulated in vacuoles, whereas [Fe]DAFC-BODIPY, -SiR and -Cy5 localised to mitochondria. [Fe]DAFC -FITC showed a uniform cytoplasmic distribution, whereas [Fe]DAFC-TAMRA was not internalised at all. Co-staining experiments with commercially available fluorescent dyes confirmed these findings. Overall, we developed a new class of fluorescent dyes that vary in intracellular fungal targeting , thereby providing novel tools for live-cell imaging applications for Aspergillus fumigatus.
Play fighting social networks do not predict injuries from later aggression
Authors: Turner Simon P. , Weller Jennifer E. , Camerlink Irene , Arnott Gareth , Choi Taegyu , Doeschl-Wilson Andrea , Farish Marianne , Foister Simone 
Published: September 2020

AbstractEarly play fighting mimics later aggression in many species, and may, therefore, be expected to reduce costs from later aggressive interactions. Using social network analysis (SNA) the effect of a central play fighting network position on later skin lesions from aggression was assessed in domestic pigs. Piglets (n = 263) were kept in litter groups or socialised pre-weaning with another litter to enhance play fighting experience. Play fighting was recorded for 1.5 h per day over 6 days pre-weaning. Play fighting network centrality was quantified using measures of individual network position and entire network structure (degree, eigenvector, betweenness, clustering coefficient). Skin lesions from aggression were counted after a dyadic contest and at 24 h and 3 weeks following group mixing. Pigs with play fighting interactions with many partners experienced fewer lesions from the dyadic contest (in-degree, p = 0.01) and tended to received fewer lesions 3 weeks after group mixing (degree, p = 0.088) but no other play fighting centrality measures affected the number of lesions at any point. The benefits of play fighting were therefore limited to specific aggressive social contexts. The tendency of socialised piglets to play fight with non-littermates did not affect subsequent lesions. We advocate the use of SNA over approaches that only consider dyadic interactions to further our understanding of the influence of early social group interactions on later life experience.