bluuebunny/crossref_metadata_2025_split huggingface dataset:
CircZNF609/miR-134-5p/BTG-2 axis regulates proliferation and migration of glioma cell
Authors: Tong Hui , Zhao Kai , Wang Jiangjie , Xu Hui , Xiao Jianqi 
Published: November 2019

Abstract

Objectives
MicroRNAs are abundant in eukaryotic cells and play key roles in cancers. Circular RNAs (CircRNAs) served as the competing endogenous RNAs (ceRNAs) in mediating multiple cell processes. This study aims to define the role of CircRNA CircZNF609/miR-134-5p in glioma as well as the underlying regulating mechanism.

Methods
Relative expression of miR-134-5p, CircZNF609 and BTG-2 mRNA was determined by quantitative real-time PCR. Cell proliferation was analysed by CCK-8 assay. Cell migration was assessed by cell wound scratch assay. The direct regulatory of miR-134-5p on BTG-2 and CircZNF609 was verified by luciferase report gene assay.

Key findings
MiR-134-5p was significantly upregulated in glioma cells. The overexpression of miR-134-5p inhibited cell proliferation and migration of glioma cell U251 and U87. Reversely, knock-down of miR-134-5p enhanced cell proliferation and migration. Both BTG-2 and CircZNF609 are the direct targets of miR-134-5p, and their expression could be negatively regulated by miR-134-5p. CircZNF609 was significantly upregulated in U251 and U87 cells and acted as an oncogene to promote cell proliferation and cell migration of glioma cell U251 and U87.

Conclusions
These data proved that CircZNF609 served as a competing RNA to bind miR-134-5p that promoted BTG-2 expression leading to reduced proliferation and migration of glioma cell.
Resilience to Freezing in the Vegetative Cells of the Microalga Lobosphaera incisa (Trebouxiophyceae, Chlorophyta)
Authors: Kugler Amit , Kumari Puja , Kokabi Kamilya , Itkin Maxim , Malitsky Sergey , Khozin‐Goldberg Inna 
Published: November 2019

The chlorophyte microalga Lobosphaera incisa was isolated from the snowy slopes of Mt. Tateyama in Japan. This microalga stores exceptionally high amounts of the omega‐6 LC‐PUFA arachidonic acid in triacylglycerols, and therefore represents a potent photosynthetic source for this essential LC‐PUFA. Assuming that freezing tolerance may play a role in adaptation of L. incisa to specific ecological niches, we examined the capability of L. incisa to tolerate extreme sub‐zero temperatures. We report here, that the vegetative cells of L. incisa survived freezing at −20°C and −80°C (over 1 month), without cryoprotective agents or prior treatments. Cells successfully recovered upon thawing and proliferated under optimal growth conditions (25°C). However, cells frozen at −80°C showed better recovery and lower cellular ROS generation upon thawing, compared to those preserved at −20°C. Photosynthetic yield of PSII, estimated by Fv/Fm, temporarily decreased at day 1 post freezing and resumed to the original level at day 3. Interestingly, the thawed algal cultures produced a higher level of chlorophylls, exceeding the control culture. The polar metabolome of the vegetative cells comprised a range of compatible solutes, dominated by glutamate, sucrose, and proline. We posit that the presence of endogenous cryoprotectants, a rigid multilayer cell wall, the high LC‐PUFA content in membrane lipids, and putative cold‐responsive proteins may contribute to the retention of functionality upon recovery from the frozen state, and therefore for the survival under cryospheric conditions. From the applied perspective, this beneficial property holds promise for the cryopreservation of starter cultures for research and commercial purposes.
Ambulatory assessment of colonic motility using the electromagnetic capsule tracking system: Effect of opioids
Authors: Mark Esben Bolvig , Klinge Mette Winther , Grønlund Debbie , Poulsen Jakob Lykke , Schlageter Vincent , Scott S. Mark , Krogh Klaus , Drewes Asbjørn Mohr 
Published: November 2019

AbstractBackgroundOpioid treatment often causes debilitating constipation. However, it is not well described how opioids affect colonic motility and whether opioid‐induced constipation is due to either a decrease of powerful peristaltic contractions or “uncoordinated” peristalsis. The present study aims to investigate the effect of oxycodone on parameters of colonic motility and to determine whether motility is normalized by the opioid antagonist naloxegol.MethodsIn two randomized, double‐blind crossover trials, oxycodone or placebo was administered to 25 healthy males (Trial A), while another 24 healthy males were administered oxycodone with naloxegol or placebo (Trial B). Colonic motility was assessed by tracking the progression of an electromagnetic capsule throughout the large intestine. Segmental colonic transit times and capsule movements were calculated using displacement distance and velocity.Key ResultsIn Trial A, colonic transit time increased during oxycodone treatment compared with placebo (39 vs 18 hours, P < .01). Displacement during long fast antegrade movements was shorter during oxycodone treatment than with placebo (10 vs 20 cm, P = .03). In Trial B, colonic transit time was faster during oxycodone + naloxegol than during oxycodone + placebo (40 vs 55 hours, P = .049), mainly caused by an increase of the percentwise fraction of distance covered by fast movements in the left colon (P = .001).Conclusion & InferencesOxycodone treatment impaired colonic motility, manifested as increased transit time, specifically decreased long fast antegrade movements, and addition of naloxegol improved motility dynamics. In humans, the increased transit time during opioid treatment is caused by a decrease in long fast movements rather than uncoordinated peristalsis.
Precision medicine, agriculture, and genome editing: science and ethics
Authors: Moscoso Carlos G. , Potz Kelly R. , Tan Shaoyuan , Jacobson Pamala A. , Berger Kavita M. , Steer Clifford J. 
Published: November 2019

AbstractThe era of precision medicine has generated advances in various fields of science and medicine with the potential for a paradigm shift in healthcare delivery that will ultimately lead to an individualized approach to medicine. Such timely topics were explored in 2018 at a workshop held at the Third International Conference on One Medicine One Science (iCOMOS), in Minneapolis, Minnesota. A broad range of scientists and regulatory experts provided detailed insights into the challenges and opportunities associated with precision medicine and gene editing. There was a general consensus that advances in studying the genomic traits driving differential pharmacogenomics will undoubtedly enhance individualized treatments for a wide variety of diseases. Ethical considerations, societal implications, approaches for prioritizing safe and secure use of treatment modalities, and the advent of high‐throughput computing and analysis of large, complex datasets were discussed. Large biobanks, such as the All of Us Research Program and the Veterans Affairs Million Veterans Program, can aid studies of various conditions in massive cohorts of patients. As the applications of precision medicine continue to mature, the full potential and promise of these individualized approaches will continue to yield important advances in transplant medicine, oncology, public health, agriculture, pharmacology, and bioinformatics.
Identification and Sensitivity Analysis of Contagion Effects in Randomized Placebo-Controlled Trials
Authors: Imai Kosuke , Jiang Zhichao 
Published: November 2019

SummaryIn social science research, interference between units is the rule rather than the exception. Contagion represents one key causal mechanism of such spillover effects, where one's treatment affects the outcome of another individual indirectly by changing the treated unit's own outcome. Alternatively, the treatment of one individual can affect the outcome of another person through other mechanisms. We consider the identification and sensitivity analysis of contagion effects. We analyse a randomized placebo-controlled trial of the get out the vote campaign, in which canvassers were sent to randomly selected households with two registered voters but encouraged only one voter within each household to turn out in an upcoming election. To address the problem of non-compliance, the experiment includes a placebo arm, in which canvassers encourage voters to recycle. We show how to identify and estimate the average contagion and direct effects by decomposing the average spillover effect. Our analysis examines whether canvassing increases the turnout of a non-contacted voter by altering the vote intention of a contacted voter or through other mechanisms. To address the potential violation of key identification assumptions, we propose non-parametric and parametric sensitivity analyses. We find robust contagion effects among some households.
Networked Geographies of Digital Contention in Post‐Financial Crisis Ireland
Authors: Nic Lochlainn Maedhbh 
Published: November 2019

AbstractThe language of networks has become a common conceptual framework for describing contemporary, digitally‐engaged social movements. In this paper I address the subject of digital contention from a geographical perspective, using network analysis and qualitative data to explore the networked digital contention of anti‐water charges community groups in Dublin, Ireland. Focusing thematically on network fragmentation, I use places and practices as frames to understand this situated case study and make two main points. First, social media networks are constituted through choices by individuals about how to articulate place relationally to fulfil specific political and social objectives. Second, contextual and historical components of specific places can provide an explanatory mechanism for understanding points of concentration and fragmentation in the network. Network analysis is useful for visualising and interpreting digital contention but augmenting network analysis with qualitative methods of data collection allows for deeper understanding of the geographical nuances of digital contention.
Epidemiology of ophthalmic lymphoma in Canada during 1992–2010
Authors: Darwich Rami , Ghazawi Feras M , Rahme Elham , Alghazawi Nebras , Zubarev Andrei , Moreau Linda , Sasseville Denis , Burnier Miguel N , Litvinov Ivan V 
Published: November 2019

BackgroundOphthalmic lymphoma (OL) is the most common orbital tumour, particularly in older individuals. Little is known about the epidemiology and geographic distribution of OL in Canada. Descriptive demographic statistics are an important first step in understanding OL burden and are necessary to inform comprehensive national cancer prevention programmes.MethodsWe determined patterns of incidence and geographical distribution of the three major subtypes of OL: extranodal marginal zone B cell lymphoma, follicular lymphoma (FL) and diffuse large B cell lymphoma. Here, we used cases that were diagnosed during 1992–2010 using two independent population-based cancer registries, the Canadian Cancer Registry and Le Registre Québécois du Cancer (LRQC).ResultsThe OL mean annual age-standardised incidence rate for 1992–2010 was 0.65 cases per million people per year with an average annual increase in the incidence rate of 4.5% per year. The mean age of diagnosis was 65 years. OL incidence rate was the highest in the cities located along the heavily industrialised Strait of Georgia in British Columbia.ConclusionsOur data on patient age, sex and temporal trends showed similarities with data reported in the USA and Denmark. Additional studies are needed to determine whether the observed increase in OL incidence is genuine or spurious.
Weight underestimation linked to anxiety and depression in a cross-sectional study of overweight individuals in a Sami and non-Sami Norwegian population: the SAMINOR Study
Authors: Kvaløy Kirsti , Melhus Marita , Silviken Anne , Broderstad Ann Ragnhild 
Published: November 2019

ObjectivesUnderestimation of overweight/obesity may prevent weight loss attempts, resulting in further weight gain and maintenance of overweight. Mental health benefits may nevertheless surpass negative consequences. Our main objective was to study the association between underestimation of overweight/obesity and symptoms of anxiety and depression in Sami and non-Sami populations.DesignPopulation-based cross-sectional study.SettingThe SAMINOR 2 Clinical Study with participants from 10 municipalities in Northern Norway enrolled between 2012 and 2014.ParticipantsThe study included 3266 adults of multiethnic rural origin with overweight/obesity (body mass index (BMI) ≥25 kg/m2) whereof 1384 underestimated their weight (42%).Primary and secondary outcome measuresPrimary outcome measure was symptoms of anxiety and depression and secondary outcome measures were BMI and the demographic variables: sex, age, education and marital status.ResultsA higher proportion of Sami men compared with non-Sami men were obese, and reported more symptoms of anxiety and depression. More men than women, and a higher proportion of Sami women compared with non-Sami women, underestimated their weight. Multivariable-adjusted analyses showed that women were less likely to underestimate their weight compared with men (OR 0.43, 95% CI 0.33 to 0.55 in Sami and OR 0.33, 95% CI 0.26 to 0.42 in non-Sami), higher BMI was protective against weight underestimation (OR 0.72, 95% CI 0.69 to 0.75 in Sami and OR 0.63, 95% CI 0.60 to 0.67 in non-Sami), slightly higher odds of weight underestimation were observed with increasing age in both ethnic groups (OR 1.03, 95% CI 1.01 to 1.05 in Sami and OR 1.02, 95% CI 1.00 to 1.03 in non-Sami), while higher education lowered the odds in non-Sami (OR 0.69, 95% CI 0.55 to 0.87). Weight underestimation was protectively associated with anxiety and depression in Sami men (OR 0.48, 95% CI 0.27 to 0.84) and in non-Sami women (OR 0.44, 95% CI 0.25 to 0.78) adjusted for age, BMI, education and marital status.ConclusionsIndependent of ethnicity, more men than women underestimated their weight. Underestimation of weight was protectively associated with anxiety and depression in Sami men and non-Sami women.
Gastropexy can be as safe as conventional percutaneous endoscopic gastrostomy (PEG), and biomarkers do not predict short-term or long-term outcomes: a 7-year follow-up audit
Authors: Porter Ross J , McKinlay Alastair W , Metcalfe Emma L 
Published: November 2019

ObjectiveGastrostomy facilitates artificial enteral feeding but controversy exists around associated morbidity and mortality. This study aimed to report short and long-term outcomes, and identify parameters associated with overall survival.MethodsA 7-year follow-up audit was undertaken at Aberdeen Royal Infirmary, UK. All patients undergoing endoscopic gastrostomy insertion October 2011–September 2018 were included. Last follow-up was February 2019. Clinical data were prospectively collected. Blood results were retrospectively obtained from electronic records. Statistical analysis was with IBM SPSS V.25.Results691 procedures were performed over the 7-year period (520 traditional pull-through percutaneous endoscopic gastrostomy (PEG) and 171 gastropexy procedures to facilitate gastrostomy). Frequency of complications (gastrointestinal bleeding, perforation and peritonitis) was low (each n=1). Overall 7-day and 30-day mortality was 2.2% and 8.4%, respectively. One-year mortality reached 47.6%. There was no difference in survival between PEG and gastropexy procedures (p=0.410). Multivariate analysis reported increased age (p<0.001), increased alkaline phosphatase (p<0.001) and clinical indication (p=0.002) as independently associated with an increased hazard of death. Only age was moderately predictive of mortality (area under the curve 0.74, 95% CI 0.70 to 0.78, p<0.001) in the PEG group. Clinical indication was the only parameter independently associated with mortality in the gastropexy cohort (p=0.003).ConclusionEndoscopic gastrostomy placement can be safe with a low mortality and low risk of serious complications. Blood markers were not associated with short-term or long-term outcomes. Gastropexy to facilitate gastrostomy is a safe alternative to traditional pull-through PEG procedures. Future work should consider quality of life outcomes to assess the benefit of gastrostomy from a patient perspective.
Intersectional community correlates of married women’s experiences of male intimate partner physical violence in Bangladesh: a cross-sectional study
Authors: Rahman Laila , Du Mont Janice , O'Campo Patricia , Einstein Gillian 
Published: November 2019

BackgroundIn Bangladesh, little is known about community-level factors shaping married women’s experiences of male intimate partner physical violence (MIPPV); it is also unknown if these factors interact with each other. We examined the (1) association between four residential community characteristics defined by the attributes of ever married women in those communities–younger age, lower education, higher participation in earning an income and poverty; and (2) two-way interactions between these community-level MIPPV correlates.MethodsWe used a cross-sectional sample comprising 14 557 currently married women who were living with their spouses from 911 Bangladeshi communities. Data were collected during 13–22 August 2015. Conflict Tactics Scale-2 measured the outcome–women’s current MIPPV experiences; and multilevel logistic regression models predicted this outcome.ResultsFour community characteristics including higher proportions of women’s earning an income and achieving higher education were not associated with their increased likelihood of experiencing MIPPV. However, women living inhigher earning participation, higher educatedcommunities were significantly more likely to experience MIPPV than those inlower earning participation, higher educatedcommunities (predicted probability, p=0.30, 95% CI 0.26 to 0.34 vs p=0.24, 95% CI 0.22 to 0.25).ConclusionThis is the first study to examine interactions between women’s community-level MIPPV correlates in Bangladesh. Although we did not find support for the relationship between women’s most intersectional community-level locations and MIPPV, we did find a currently invisible vulnerable intersectional location:higher earning participation, higher educatedcommunities. Bangladeshi violence against women prevention policies and programmes, therefore, need to engage with these particular communities to tackle head on male responses to these locations to reduce MIPPV.
Barley or black oat silages in feeding strategies for small-scale dairy systems in the highlands of Mexico
Authors: Gómez-Miranda Aída , Estrada-Flores Julieta Gertrudis , Morales-Almaraz Ernesto , López-González Felipe , Flores-Calvete Gonzalo , Arriaga-Jordán Carlos Manuel 
Published: November 2019

High costs from external inputs in small-scale dairy systems (SSDS) and possible effects of climate change, require forage alternatives as silage for the dry season, from small-grain cereals that have short cropping cycles, winter hardiness, and good nutritional quality. The objective was to assess the provision of 10 kg dry matter (DM) cow−1d−1of barley (BLY) or black oat (BKO) silages in three treatments: T1 = 100% BLY; T2 = 50% BLY + 50% BKO; T3 = 100% BKO for milking cows. All treatments also received 4.6 kg DM cow−1d−1of concentrates and access to pasture. Nine Holstein cows in groups of three were randomly assigned to a 3 × 3 Latin square design repeated three times, with 14 d experimental periods. Measurements of animal variables and sampling for chemical analyses of feeds were done during the last 4 d of each period. Feeding costs were by partial budgets. There were no differences (P > 0.05) for milk yield, milk fat and protein content, milk urea nitrogen, body condition score, or live weight. The cost of BLY silage was 8% less than BKO silage. T1 had the higher margin over cost of feeds followed by T2. Both silages alone or in combination are viable options for SSDS, as there were no differences in performance, or in feeding costs or margins.
Wear performance of selective laser melted Ti-6Al-4V alloy in situ modified with oxygen and boron
Authors: Jackson Ben , Torrens Rob , Bolzoni Leandro , Yang Fei , Ross Nigel , Fry Mike 
Published: November 2019

Titanium alloys have poor wear performance, with severe adhesive wear and three-body abrasion being dominant mechanisms. To extend the use of titanium to applications demanding better wear properties, modifications can be made to the alloys. This can include the addition of hard particulates or interstitial strengthening, by increasing the oxygen or nitrogen content. The metal additive manufacturing process of selective laser melting (SLM) has been shown to enable manufacture of these modified titanium alloys in situ. In this study, small amounts of boron and titanium dioxide powders were added to Ti-6Al-4V (Ti64) and processed using SLM. To compare wear performance of these modified materials, reciprocating pin on plate tests in brine solution were performed. Increased oxygen content increased the hardness of the material, which reduced wear. The presence of boron increased wear in the short term but reduced the long-term wear rate. Incorporating of oxygen and boron has been shown to improve the saline solution wear properties of Ti64 against silicon nitride.
Understanding the Knowledge Gaps of Software Engineers
Authors: Garousi Vahid , Giray Gorkem , Tuzun Eray 
Published: November 2019

Context:Knowledge level and productivity of the software engineering (SE) workforce are the subject of regular discussions among practitioners, educators, and researchers. There have been many efforts to measure and improve the knowledge gap between SE education and industrial needs.Objective:Although the existing efforts for aligning SE education and industrial needs have provided valuable insights, there is a need for analyzing the SE topics in a more “fine-grained” manner; i.e., knowing that SE university graduates should know more about requirements engineering is important, but it is more valuable to know the exact topics of requirements engineering that are most important in the industry.Method:We achieve the above objective by assessing the knowledge gaps of software engineers by designing and executing an opinion survey on levels of knowledge learned in universities versus skills needed in industry. We designed the survey by using the SE knowledge areas (KAs) from the latest version of the Software Engineering Body of Knowledge (SWEBOK v3), which classifies the SE knowledge into 12 KAs, which are themselves broken down into 67 subareas (sub-KAs) in total. Our analysis is based on (opinion) data gathered from 129 practitioners, who are mostly based in Turkey.Results:Based on our findings, we recommend that educators should include more materials on software maintenance, software configuration management, and testing in their SE curriculum. Based on the literature as well as the current trends in industry, we provide actionable suggestions to improve SE curriculum to decrease the knowledge gap.