bluuebunny/crossref_metadata_2025_split huggingface dataset:
High-throughput immunogenetic typing of koalas suggests possible link between MHC alleles and cancers
Authors: Quigley Bonnie L. , Tzipori Galit , Nilsson Karen , Timms Peter 
Published: October 2020

AbstractCharacterizing the allelic diversity within major histocompatibility complex (MHC) genes is an important way of determining the potential genetic resilience of a population to infectious and ecological pressures. For the koala (Phascolarctos cinereus), endemic diseases, anthropogenic factors and climate change are all placing increased pressure on this vulnerable marsupial. To increase the ability of researchers to study MHC genetics in koalas, this study developed and tested a high-throughput immunogenetic profiling methodology for targeting MHC class I UA and UC genes and MHC class II DAB, DBB, DCB and DMB genes in a population of 82 captive koalas. This approach was validated by comparing the determined allelic profiles from 36 koala family units (18 dam-sire-joey units and 18 parent-joey pairs), finding 96% overall congruence within family profiles. Cancers are a significant cause of morbidity in koalas and the risk factors remain undetermined. Our analysis of this captive population revealed several novel MHC alleles, including a potential link between the DBB*03 allele and a risk of developing cancer. This method offers a reliable, high-throughput protocol for expanded study into koala immunogenetics.
A Protocol is not Enough: Enhanced Recovery Program‐Based Care and Clinician Adherence Associated with Shorter Stay After Colorectal Surgery
Authors: Byrne Ben E. , Faiz Omar D. , Bottle Alex , Aylin Paul , Vincent Charles A. 
Published: October 2020

AbstractBackgroundRandomised trials have shown an Enhanced Recovery Program (ERP) can shorten stay after colorectal surgery. Previous research has focused on patient compliance neglecting the role of care providers. National data on implementation and adherence to standardised care are lacking. We examined care organisation and delivery including the ERP, and correlated this with clinical outcomes.MethodsA cross‐sectional questionnaire was administered to surgeons and nurses in August–October 2015. All English National Health Service Trusts providing elective colorectal surgery were invited. Responses frequencies and variation were examined. Exploratory factor analysis was performed to identify underlying features of care. Standardised factor scores were correlated with elective clinical outcomes of length of stay, mortality and readmission rates from 2013–15.Results218/600 (36.3%) postal responses were received from 84/90 (93.3%) Trusts that agreed to participate. Combined with email responses, 301 surveys were analysed. 281/301 (93.4%) agreed or strongly agreed that they had a standardised, ERP‐based care protocol. However, 182/301 (60.5%) indicated all consultants managed post‐operative oral intake similarly. After factor analysis, higher hospital average ERP‐based care standardisation and clinician adherence score were significantly correlated with reduced length of stay, as well as higher ratings of teamwork and support for complication management.ConclusionsStandardised, ERP‐based care was near universal, but clinician adherence varied markedly. Units reporting higher levels of clinician adherence achieved the lowest length of stay. Having a protocol is not enough. Careful implementation and adherence by all of the team is vital to achieve the best results.
Prophylaxis, diagnosis and therapy of infections in patients undergoing high-dose chemotherapy and autologous haematopoietic stem cell transplantation. 2020 update of the recommendations of the Infectious Diseases Working Party (AGIHO) of the German Society of Hematology and Medical Oncology (DGHO)
Authors: Christopeit Maximilian , Schmidt-Hieber Martin , Sprute Rosanne , Buchheidt Dieter , Hentrich Marcus , Karthaus Meinolf , Penack Olaf , Ruhnke Markus , Weissinger Florian , Cornely Oliver A. , Maschmeyer Georg 
Published: October 2020

AbstractTo ensure the safety of high-dose chemotherapy and autologous stem cell transplantation (HDC/ASCT), evidence-based recommendations on infectious complications after HDC/ASCT are given. This guideline not only focuses on patients with haematological malignancies but also addresses the specifics of HDC/ASCT patients with solid tumours or autoimmune disorders. In addition to HBV and HCV, HEV screening is nowadays mandatory prior to ASCT. For patients with HBs antigen and/or anti-HBc antibody positivity, HBV nucleic acid testing is strongly recommended for 6 months after HDC/ASCT or for the duration of a respective maintenance therapy. Prevention of VZV reactivation by vaccination is strongly recommended. Cotrimoxazole for the prevention of Pneumocystis jirovecii is supported. Invasive fungal diseases are less frequent after HDC/ASCT, therefore, primary systemic antifungal prophylaxis is not recommended. Data do not support a benefit of protective room ventilation e.g. HEPA filtration. Thus, AGIHO only supports this technique with marginal strength. Fluoroquinolone prophylaxis is recommended to prevent bacterial infections, although a survival advantage has not been demonstrated.
Lactobacillus Cell Surface Proteins Involved in Interaction with Mucus and Extracellular Matrix Components
Authors: Muscariello Lidia , De Siena Barbara , Marasco Rosangela 
Published: October 2020

AbstractThe gut microbiota is a complex microbial ecosystem where bacteria, through mutual interactions, cooperate in maintaining of wellbeing and health. Lactobacilli are among the most important constituents of human and animal intestinal microbiota and include many probiotic strains. Their presence ensures protection from invasion of pathogens, as well as stimulation of the immune system and protection of the intestinal flora, often exerted through the ability to interact with mucus and extracellular matrix components. The main factors responsible for mediating adhesion of pathogens and commensals to the gut are cell surface proteins that recognize host targets, as mucus layer and extracellular matrix proteins. In the last years, several adhesins have been reported to be involved in lactobacilli–host interaction often miming the same mechanism used by pathogens.
“Topographic Shift”: a new digital approach to evaluating topographic changes of the female breast
Authors: Lotter Luisa , Brébant Vanessa , Eigenberger Andreas , Hartmann Robin , Mueller Karolina , Baringer Magnus , Prantl Lukas , Schiltz Daniel 
Published: October 2020

Abstract
Purpose
To assess precise topographic changes of the breast, objective documentation and evaluation of pre- and postoperative results are crucial. New technologies for mapping the body using digital, three-dimensional surface measurements have offered novel ways to numerically assess the female breast. Due to the lack of clear demarcation points of the breast contour, the selection of landmarks on the breast is highly dependent on the examiner, and, therefore, is prone to error when conducting before-after comparisons of the same breast. This study describes an alternative to volumetric measurements, focusing on topographic changes of the female breast, based on three-dimensional scans.

Method
The study was designed as an interventional prospective study of 10 female volunteers who had planned on having aesthetic breast augmentation with anatomical, textured implants. Three dimensional scans of the breasts were performed intraoperatively, first without and then with breast implants. The topographic change was determined as the mean distance between two three-dimensional layers before and after augmentation. This mean distance is defined as the Topographic Shift.

Results
The mean implant volume was 283 cc (SD = 68.6 cc, range = 210–395 cc). The mean Topographic Shift was 7.4 mm (SD = 1.9 mm, range = 4.8–10.7 mm). The mean Topographic Shifts per quadrant were: I: 8.0 mm (SD = 3.3 mm); II: 9.2 mm (SD = 3.1 mm); III: 6.9 mm (SD = 3.5 mm); IV: 1.9 mm (SD = 4.3 mm).

Conclusion
The Topographic Shift, describing the mean distance between two three-dimensional layers (for example before and after a volume changing therapy), is a new approach that can be used for assessing topographic changes of a body area. It was found that anatomical, textured breast implants cause a topographic change, particularly on the upper breast, in quadrant II, the décolleté.
Long-term results of cochlear implantation in children with congenital single-sided deafness
Authors: Rauch Ann-Kathrin , Arndt Susan , Aschendorff Antje , Beck Rainer , Speck Iva , Ketterer Manuel Christoph , Jakob Till Fabian , Hassepass Frederike 
Published: October 2020

Abstract
Purpose
The purpose of this retrospective study was to investigate the outcome and critical age of cochlear implantation in congenital single-sided deafness (SSD).

Methods
11 children with congenital SSD were implanted with a cochlear implant (CI). Auditory performance was measured through the results of speech discrimination, subjective assessment by the Categories of auditory performance (CAP) score, the Speech, Spatial and Qualities scale questionnaire (SSQ) and the German version of the IOI-HA [Internationales Inventar zur Evaluation von Hörgeräten (IIEH, version for CI)].

Results
Long-term follow-up [median: 3 years and 5 months (3;5 years)] revealed that nine children use their CI (> 8 h/day) and two became nonusers. In children aged below 3;2 years at surgery, there was a substantial long-term increase in speech discrimination and subjective benefit. Children over 4;4 years of age at CI surgery improved partially in audiological/subjective measurements. Among children above 5 years, the SSQ score did not improve despite further slight improvement in speech discrimination long-term.

Conclusion
Our data suggest a critical age for CI surgery below 3 years in children with congenital SSD for successful hearing rehabilitation. It is mandatory to identify children with SSD as early as bilaterally deaf children.
Conditional knockdown of integrin beta-3 reveals its involvement in osteolytic and soft tissue lesions of breast cancer skeletal metastasis
Authors: Kovacheva Marineta , Zepp Michael , Berger Stefan , Berger Martin R. 
Published: October 2020

AbstractIntegrin β3 (ITGB3) is probably related to skeletal metastasis, which is the most frequent complication in breast cancer progression. We aimed to define its role and suitability as target for anti-metastatic therapy. We generated two MDA-MB-231 cell clones with conditional miRNA-mediated ITGB3 knockdown for analyzing the resulting effects in vitro regarding mRNA expression, proliferation and migration, as well the impact on skeletal metastasis in a nude rat model. Furthermore, ITGB3 levels were analyzed in exosomes from plasma of rats with skeletal metastases, and from MDA-MB-231 cells incubated with these vesicles, as well as from exosomes secreted by cells with conditional ITGB3 knockdown. This inhibition of ITGB3 expression decreased cellular proliferation and more distinctly inhibited cellular migration. Reduction and even complete remissions of respective soft tissue and osteolytic lesions were detected after ITGB3 knockdown in vivo. Furthermore, ITGB3 levels were increased in exosomes isolated from plasma of rats harboring MDA-MB-231 lesions as well as in respective cells incubated with these vesicles in vitro. ITGB3 was distinctly decreased in exosomes from cells with ITGB3 knockdown. The observed in vitro and in vivo anti-ITGB3 effects can be explained by downregulation of specific genes, which have roles in angiogenesis (NPTN, RRM2), tumor growth (NPTN), energy metabolism (ISCA1), cytokinesis (SEPT11), migration (RRM2, STX6), cell proliferation, invasiveness, senescence, tumorigenesis (RRM2) and vesicle trafficking (SEPT11, STX6). ITGB3 has a role in breast cancer skeletal metastasis via gene expression modulation, as mirrored for ITGB3 in exosomes, thus it could serve as target for anti-metastatic therapy.
Outpatient before inpatient—the good, the bad and the ugly
Authors: Vuong Kim Tai , Guglielmetti Laura C. , Albert Thomas G. , Brillat Arce Waldemar , Staerkle Ralph F. , Vuille-dit-Bille Raphael N. 
Published: October 2020

Summary
Background
In January 2019 the Swiss Federal Department of Home Affairs defined a group of six selected surgical interventions to be performed on an outpatient basis. The aim of this paper is to assess surgeons’ opinions on patient safety, costs and treatment based on this new regulation.

Methods
An online survey was sent electronically to all 942 members of the Swiss Society of Surgery between August and October 2019.

Results
About half of the participants think the new regulation could harm patients (52%) and will lead to lower patient satisfaction (49%). Whereas half of the participants expect a reduction in health care costs (52%), most expect surgeons to earn less due to the new regulation (82%). About three quarters (73%) of the participants expect the new regulation to negatively affect surgical resident education. More than half (62%) of the participants assume that diagnoses allowing reimbursement for inpatient treatment (such as, e.g., bilateral instead of unilateral inguinal hernia) could be made more generously. Accordingly, 70% assume that the new regulation may result in not necessarily indispensable or possibly unnecessarily extended interventions (such as, e.g., bilateral inguinal hernia repair). Furthermore, most (86%) participants fear that the new regulation could possibly lead to hospitals/surgeons rejecting patients.

Conclusion
Whereas about half of the participants expect a reduction in health care costs, about two thirds fear that more generous diagnoses and not necessarily indispensable or possibly unnecessarily extended interventions could be performed due to the new regulation demanding outpatient care for said surgical interventions.
Superbase-based protic ionic liquids for cellulose filament spinning
Authors: Elsayed Sherif , Hummel Michael , Sawada Daisuke , Guizani Chamseddine , Rissanen Marja , Sixta Herbert 
Published: October 2020

Abstract
Lyocell fibers have received increased attention during the recent years. This is due to their high potential to satisfy the rising market demand for cellulose-based textiles in a sustainable way. Typically, this technology adopts a dry-jet wet spinning process, which offers regenerated cellulose fibers of excellent mechanical properties. Compared to the widely exploited viscose process, the lyocell technology fosters an eco-friendly process employing green direct solvents that can be fully recovered with low environmental impact. N-methylmorpholine N-oxide (NMMO) is a widely known direct solvent that has proven its success in commercializing the lyocell process. Its regenerated cellulose fibers exhibit higher tenacities and chain orientation compared to viscose fibers. Recently, protic superbase-based ionic liquids (ILs) have also been found to be suitable solvents for lyocell-type fiber spinning. Similar to NMMO, fibers of high mechanical properties can be spun from the cellulose-IL solutions at lower spinning temperatures. In this article, we study the different aspects of producing regenerated cellulose fibers using NMMO and relevant superbase-based ILs. The selected ILs are 1,5-diazabicyclo[4.3.0]non-5-ene-1-ium acetate ([DBNH]OAc), 7-methyl-1,5,7-triazabicyclo[4.4.0] dec-5-enium acetate ([mTBDH]OAc) and 1,8-diazabicyclo[5.4.0]undec-7-enium acetate ([DBUH]OAc). All ILs were used to dissolve a 13 wt% (PHK) cellulose pulp. The study covers the fiber spinning process, including the rheological characterization of the various cellulose solutions. Moreover, we discuss the properties of the produced fibers such as mechanical performance, macromolecular properties and morphology.

Graphic abstract
The two dimensions of Italian academics’ public engagement
Authors: Anzivino Monia , Ceravolo Flavio A. , Rostan Michele 
Published: October 2020

AbstractThe article aims at investigating Italian academics’ Public Engagement highlighting its two dimensions, namely “Local Community Engagement” (LCE) and “General Political Engagement” (GPE). It is based on the results of a national survey on academics’ third mission activities carried out in the year 2015/2016 collecting information from a representative sample of 5,123 respondents working at 62 universities, with a response rate of 34.2%. In addition to detecting distinct dimensions of academics’ Public Engagement, data analysis shows that Italian academics are much more involved in LCE activities than in GPE ones. Although both LCE and GPE are influenced by many common factors, such as academic rank, discipline and being involved in other third mission activities, they also display peculiar traits: LCE activities are more likely to involve academic women while GPE are more likely to be carried out by older academics; LCE activities appear to be context-dependent while GPE activities are not. Hence, the article offers a contribution to a better understanding of the different goals of Public Engagement activities and of their spatial dimension.
Empirical analysis of session-based recommendation algorithms
Authors: Ludewig Malte , Mauro Noemi , Latifi Sara , Jannach Dietmar 
Published: October 2020

AbstractRecommender systems are tools that support online users by pointing them to potential items of interest in situations of information overload. In recent years, the class of session-based recommendation algorithms received more attention in the research literature. These algorithms base their recommendations solely on the observed interactions with the user in an ongoing session and do not require the existence of long-term preference profiles. Most recently, a number of deep learning-based (“neural”) approaches to session-based recommendations have been proposed. However, previous research indicates that today’s complex neural recommendation methods are not always better than comparably simple algorithms in terms of prediction accuracy. With this work, our goal is to shed light on the state of the art in the area of session-based recommendation and on the progress that is made with neural approaches. For this purpose, we compare twelve algorithmic approaches, among them six recent neural methods, under identical conditions on various datasets. We find that the progress in terms of prediction accuracy that is achieved with neural methods is still limited. In most cases, our experiments show that simple heuristic methods based on nearest-neighbors schemes are preferable over conceptually and computationally more complex methods. Observations from a user study furthermore indicate that recommendations based on heuristic methods were also well accepted by the study participants. To support future progress and reproducibility in this area, we publicly share the session-rec evaluation framework that was used in our research.
Large-area flexible nanostripe electrodes featuring plasmon hybridization engineering
Authors: Mennucci Carlo , Chowdhury Debasree , Manzato Giacomo , Barelli Matteo , Chittofrati Roberto , Martella Christian , Buatier de Mongeot Francesco 
Published: October 2020

AbstractMultifunctional flexible Au electrodes based on one-dimensional (1D) arrays of plasmonic gratings are nanofabricated over large areas with an engineered variant of laser interference lithography optimized for low-cost transparent templates. Au nanostripe (NS) arrays achieve sheet resistance in the order of 20 Ohm/square on large areas (∼ cm2) and are characterized by a strong and dichroic plasmonic response which can be easily tuned across the visible (VIS) to near-infrared (NIR) spectral range by tailoring their cross-sectional morphology. Stacking vertically a second nanostripe, separated by a nanometer scale dielectric gap, we form near-field coupled Au/SiO2/Au dimers which feature hybridization of their localized plasmon resonances, strong local field-enhancements and a redshift of the resonance towards the NIR range. The possibility to combine excellent transport properties and optical transparency on the same plasmonic metasurface template is appealing in applications where low-energy photon management is mandatory like e.g., in plasmon enhanced spectroscopies or in photon harvesting for ultrathin photovoltaic devices. The remarkable lateral order of the plasmonic NS gratings provides an additional degree of freedom for tailoring the optical response of the multifunctional electrodes via the excitation of surface lattice resonances, a Fano-like coupling between the broad localised plasmonic resonances and the collective sharp Rayleigh modes.
Clinical expert consensus document on rotational atherectomy from the Japanese association of cardiovascular intervention and therapeutics
Authors: Sakakura Kenichi , Ito Yoshiaki , Shibata Yoshisato , Okamura Atsunori , Kashima Yoshifumi , Nakamura Shigeru , Hamazaki Yuji , Ako Junya , Yokoi Hiroyoshi , Kobayashi Yoshio , Ikari Yuji 
Published: October 2020

AbstractRotational atherectomy (RA) has been widely used for percutaneous coronary intervention (PCI) to severely calcified lesions. As compared to other countries, RA in Japan has uniquely developed with the aid of greater usage of intravascular imaging devices such as intravascular ultrasound (IVUS) or optical coherence tomography (OCT). IVUS has been used to understand the guidewire bias and to decide appropriate burr sizes during RA, whereas OCT can also provide the thickness of calcification. Owing to such abundant experiences, Japanese RA operators modified RA techniques and reported unique evidences regarding RA. The Task Force on Rotational Atherectomy of the J apanese Association of Cardiovascular Intervention and Therapeutics (CVIT) has now proposed the expert consensus document to summarize the contemporary techniques and evidences regarding RA.
Depression, anxiety, and suicidal ideation in young adults 5 years after undergoing bariatric surgery as adolescents
Authors: Järvholm Kajsa , Olbers Torsten , Peltonen Markku , Marcus Claude , Flodmark Carl-Erik , Gronowitz Eva , Dahlgren Jovanna , Karlsson Jan 
Published: October 2020

Abstract
Purpose
Metabolic and bariatric surgery (MBS) is increasingly used in adolescents. The aim was to explore symptoms of depression and anxiety in young adults over 5 years’ follow-up after undergoing MBS.

Methods
Beck Depression Inventory-2 and the Beck Anxiety Inventory were used to assess symptoms of depression and anxiety in 62 patients 1, 2, and 5 years after having Roux-en-Y gastric bypass at 13–18 years of age. Mental health, eating-related problems, and weight outcomes were tested for association with suicidal ideation at the 5-year follow-up.

Results
At the 5-year follow-up, the mean score for depression was 11.4 (± 12.4), indicating minimal symptoms of depression. The mean score for anxiety was 12.82 (± 11.50), indicating mild anxiety symptoms. Still, several participants reported moderate or severe symptoms of depression (26%) and anxiety (32%). Women reported more symptoms than men (P = 0.03 and 0.04). No significant changes were found in self-reported symptoms of depression and anxiety between the 1-year and the 5-year follow-up (P = 0.367 and 0.934). Suicidal ideation was reported by 16% at the 5-year follow-up. Participants reporting suicidal ideation had lost significantly less excess weight than participants without suicidal ideation (P = 0.009).

Conclusion
Five years after adolescent MBS, a substantial minority still struggles with mental health issues, and women are more burdened than men. Our results indicate an association between less optimal weight loss and suicidal ideation 5 years after MBS. The findings emphasize the importance of offering long-term follow-up and mental health treatment several years after MBS.

Level of evidence
Level III, cohort study.

Clinical trial registration
The study is registered with ClinicalTrials.gov (NCT00289705). First posted February 10, 2006.