bluuebunny/crossref_metadata_2025_split huggingface dataset:
Human papillomavirus and survival of patients with sinonasal squamous cell carcinoma
Authors: Oliver Jamie R. , Lieberman Seth M. , Tam Moses M. , Liu Cheng Z. , Li Zujun , Hu Kenneth S. , Morris Luc G. T. , Givi Babak 
Published: December 2019

BackgroundTo the authors' knowledge, the question of whether human papillomavirus (HPV) infection is associated with outcomes in patients with sinonasal squamous cell carcinoma (SNSCC) is not well studied at this time. In the current study, the authors investigated patterns of HPV testing and its association with survival in patients with SNSCC using the National Cancer Data Base.MethodsThe authors selected all SNSCC cases diagnosed between 2010 and 2016. HPV testing practices, clinicodemographic factors, treatments, and survival were analyzed. Multivariable Cox regression and propensity score–matched survival analyses were performed.ResultsA total of 6458 SNSCC cases were identified. Of these, only 1523 cases (23.6%) were tested for HPV and included in the current study. The median patient age was 64 years and the majority had advanced stage tumors (overall AJCC stage III‐IV, 721 patients; 62.1%). HPV‐positive SNSCC comprised 31.5% (447 of 1418 cases) of the final study cohort. Among 15 hospitals that routinely tested nonoropharyngeal SCCs for HPV, the percentage of HPV‐positive SNSCCs was smaller (24.6%; P = .04). Patients with HPV‐positive SNSCC were younger (aged 60 years vs 65 years; P < .001), with tumors that were more likely to be high grade (55.3% vs 41.7%; P < .001), and attributed to the nasal cavity (62.2% vs 44.0%; P < .001). HPV‐positive SNSCC was associated with significantly improved overall survival in multivariable regression analysis (hazard ratio, 0.45; 95% CI, 0.28‐0.72 [P = .001]) and propensity score–matched (hazard ratio, 0.61; 95% CI, 0.38‐0.96 [P = .03]) analyses controlling for clinicodemographic and treatment factors.ConclusionsCurrently, only a minority of patients with SNSCC are tested for HPV. However, a sizable percentage of SNSCC cases may be HPV related; furthermore, HPV‐positive SNSCC is associated with improved overall survival. Routine HPV testing may be warranted in patients with SNSCC.
Hormone receptor and HER2 assessment in breast carcinoma metastatic to bone: A comparison between FNA cell blocks and decalcified core needle biopsies
Authors: Zeng Jennifer , Piscuoglio Salvatore , Aggarwal Gitika , Magda Joanna , Friedlander Maria A. , Murray Melissa , Akram Muzaffar , Reis‐Filho Jorge S. , Weigelt Britta , Edelweiss Marcia 
Published: December 2019

BackgroundEstrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2 (HER2) immunohistochemistry (IHC) guide the clinical management of breast cancer metastases. Decalcification of bone core needle biopsies (CNBs) can affect IHC. In the current study, the authors sought to define whether fine‐needle aspiration (FNA) would be a better alternative to CNB for reliable IHC.MethodsPatients with breast cancer metastases to bone that were sampled by both CNB and FNA were selected. ER, PR, and HER2 were performed in FNA cell blocks (FNA‐CBs) and concurrent decalcified CNBs. Discrepancies were classified as minor when there was a difference of up to 30% nuclear staining in IHC for ER and PR between paired samples and as major when a clinically relevant change was observed (ie, positive vs negative). Quantitative reverse transcriptase–polymerase chain reaction of ESR1 messenger RNA levels was performed on FNA/CNB pairs with discrepancies for ER IHC. IHC status of the primary breast carcinoma was recorded.ResultsConcordance rates for ER, PR, and HER2 were 89%, 67%, and 93%, respectively, between FNA‐CB and CNB pairs from 27 patients. Major discrepancies were noted in approximately 11% of FNA/CNB pairs for ER IHC and in 33% of FNA/CNB pairs for PR. ESR1 messenger RNA levels of FNA/CNB matched samples were similar and did not explain the differences in ER IHC expression in the majority of cases. Two of 27 FNA/CNB pairs had different results for HER2 IHC that changed from negative on CNB to equivocal (2+) on FNA‐CB. Both cases had prior HER2 amplification by fluorescence in situ hybridization.ConclusionsFNA‐CB and CNB appear to constitute acceptable methods for the assessment of ER, PR, and HER2 for clinical decision making.
Cellular automaton model considering the effect of brake light and traffic light at the intersection
Authors: Chen Yu‐Feng , Dong Zhang , Che Kai , Xiang Zheng‐Tao 
Published: December 2019

SummaryTraffic flow modeling of urban traffic on a single‐lane road with one traffic light operating as a bottleneck is the basis of research on urban road network. By introducing the brake light rules into ChSch model, we proposed a new cellular automaton traffic flow model of intersection considering the effect of brake light and traffic light to avoid running a red light and rear‐end collision. The first vehicle of the vehicle queue formed in front of the stop line at red light (FVoQ) is focused, and the evolution characteristics of intersection traffic flow are analyzed. Results show that the proposed model is more realistic than ChSch model in the following aspects: (a) The FVoQ decelerates more smoothly with the proposed model. The behavior of smooth deceleration reflects the driver's desire of comfortable driving in real traffic. (b) The proportion of the FVoQ's great and sudden deceleration to avoid running a red light is greatly reduced with the proposed model. In ChSch model, the proportion is larger than 80%, while in the proposed model, the proportion is less than 1%. The behavior of great and sudden deceleration reflects the aggressive driving pattern of a few drivers in real traffic. (c) The rear‐end collision accident rate caused by FVoQ's aggressive driving behaviors is greatly reduced with the proposed model, which conforms more to the real traffic.
Medullary thyroid carcinoma co‐existent with Hashimoto's thyroiditis diagnosed by a comprehensive cytological approach
Authors: Malpani Sheetal , Tandon Ashwani , Panwar Hemlata , Khurana Ujjawal , Kapoor Neelkamal , Behera Ganakalyan , Gupta Vikas 
Published: December 2019

AbstractFine‐needle aspiration cytology (FNAC) is a preliminary test for the diagnosis of thyroid lesions. We hereby report a rare case of medullary thyroid carcinoma (MTC) co‐existing with Hashimoto's thyroiditis (HT). This case was substantiated with ancillary tests on cytology material to give a novel insight. A 60‐year‐old female presented clinically with diffuse enlargement of the thyroid, and right‐side nodule on ultrasonography. FNAC of the isthmic area showed features of HT, while cytology of right‐side nodule displayed sheets of plasmacytoid cells and frequent scattered large bizarre and pleomorphic cells, lymphoglandular bodies and pale eosinophilic material. The differential diagnosis of MTC with co‐existent HT or high‐grade lymphoma was considered. To establish the diagnosis, serum calcitonin and ancillary studies on aspirated material were carried out. High serum calcitonin (7251 pg/mL), Congophilia on smears, and CD 45−ve; CD56+ve expression of tumor cells on flowcytometric analysis established the diagnosis of MTC over lymphoma. This diagnosis was further confirmed upon histopathology. Ancillary studies on aspirated material established the diagnosis of MTC and excluded the diagnosis of lymphoma. Establishing the correct diagnosis was cardinal in such a scenario as these diseases have extremely diverse management.
Perceived wintering latitude determines timing of song output in a migratory bird
Authors: Brewer Dustin E. , McGill Clint A. , Fudickar Adam M. 
Published: December 2019

AbstractMigratory bird populations frequently consist of individuals that overwinter variable distances from the breeding site. Seasonal changes in photoperiod, which varies with latitude, underlie seasonal changes in singing frequency in birds. Therefore, migratory populations that consist of individuals that overwinter at different latitudes with large overwintering ranges could experience within‐population variation in seasonal production of song. To test the influence of overwintering latitude on intrapopulation variance in song production in the spring, we subjected two groups of Eastern Song Sparrows (Melospiza melodia melodia) from the same partially migratory breeding population to different photoperiodic schedules associated with a 1,300‐km difference in overwintering location. One group remained on the natural photoperiodic schedule of the breeding site (resident group) while the other group experienced a nonbreeding photoperiod that mimicked a southern migration in the fall followed by a northern migration back to the breeding site in the spring (migratory group). We compared song output between the two groups in three different stages (nonbreeding, prebreeding, and breeding). Little singing occurred during nonbreeding stage sample dates (20 November, 6 December) for the resident group, and no singing occurred for the migrant group. During the prebreeding stage (27 January, 7 February), significantly more singing occurred in the resident group than in the migrant group. During the breeding stage (21 March, 4 April), after a simulated migration for the migrants, song output was similar in both groups. These results suggest that within‐population variation in wintering latitude may contribute to variation in seasonal changes in singing behavior, which may covary with readiness to breed. Studies utilizing confirmed migrants and residents, rather than merely simulated migrants and residents, are also needed to better understand these processes.
An efficient blockchain‐based batch verification scheme for vehicular ad hoc networks
Authors: Yang Yi , He Debiao , Wang Huaqun , Zhou Lu 
Published: December 2019

AbstractAs an important branch of the Internet of Things (IoT), Vehicular Ad Hoc Networks (VANETs) have attracted wide attention from industrial and academic. Due to an opening wireless channel, the security and efficiency of VANETs have also become serious. To enhance the security and efficiency, various batch verification schemes have been proposed. However, most of those schemes suffer from the complicated certificate management problem or different kinds of attacks. Also,the bilinear pairing which is intractable for lightweight devices in VANETs usually be used inthose schemes. To solve the above problems, we propose an efficient blockchain‐based batch verification scheme for VANETs using the Elliptic Curve Cryptography (ECC). We also provide security analysis to show its ability to resist current known attacks. Besides, we have implemented corresponding blockchain system and the performance analysis shows that it is suitable for VANETs.
Social participation in head and neck cancer survivors with swallowing disorder: World Health Organization Disability Assessment Schedule 2.0 study
Authors: Lee Yu‐Hao , Goo‐Yoshino Shari , Lew Henry L. , Chi Wen‐Chou , Yen Chia‐Feng , Liao Hua‐Fang , Chen Shih‐Ching , Liou Tsan‐Hon 
Published: December 2019

AbstractBackgroundSocial function and quality of life were negatively impacted by the sequelae of treatment for the disease in the head and neck cancer survivor with swallowing disorder.MethodData from a total of 1023 survivors between July 2012 and November 2017 were collected from the Taiwan Data Bank of Persons with Disability. Nonparametric rank F test was used to analyze the influence of different variables on social participation.Results(a) All WHODAS (World Health Organization Disability Assessment Schedule) 2.0 scores revealed significantly increased difficulty when swallowing impairment increased (P < .001). (b) Unemployment, institutionalization, severity of swallowing impairment, cancer sites, and WHODAS 2.0 score of domains 1 to 4 were negatively related to social participation, while living in rural area is related to increased social participation.ConclusionsSocial participation was negatively affected by many variables in this population. Among these variables, cancer sites and WHODAS 2.0 score of domains 1 to 4 were the strongest factors.
Parenting and Children's Executive Function Stability Across the Transition to School
Authors: Helm Abigail F. , McCormick Sarah A. , Deater‐Deckard Kirby , Smith Cynthia L. , Calkins Susan D. , Bell Martha Ann 
Published: December 2019

AbstractWhen children transition to school between the ages of 4 and 6 years, they must learn to control their attention and behaviour to be successful. Concurrently, executive function (EF) is an important skill undergoing significant development in childhood. To understand changes occurring during this period, we examined the role of parenting in the development of children's EF from 4 to 6 years old. Participants were mother and child dyads (N= 151). Children completed cognitive tasks to assess overall EF at age 4 and age 6. At both time points, mothers and children completed interaction tasks which were videotaped and coded to assess various parenting dimensions. Results indicated that children with high EF at age 4 were more likely to have high EF at age 6. In addition, results suggested that higher levels of positive parenting across the transition to school promote stability of individual differences in EF.HighlightsThis study investigated the association between parenting and the development of children's executive function (EF) skills across the transition to school.Children's EF and mothers' parenting were assessed at age 4 and age 6.The stability of individual differences in EF was linked to positive parenting.Parenting practises may be related to the stability of individual difference in EF across the transition to school.
Study on microstructure, microhardness, bioactivity, and biocompatibility of La2O3‐containing bioceramic coating doping SiO2 fabricated by laser cladding
Authors: Fu Qi , Liu Qibin , Li Long , Li Xingmao , Gu Huaizhang , Sheng Bo , Yang Bangcheng 
Published: December 2019

AbstractTo solve the lack of strength of calcium phosphate ceramic coatings in load‐bearing applications, gradient Ca‐P bioceramic coatings doped with La2O3 and SiO2 are fabricated by laser cladding on Ti‐6Al‐4 V. The effect of SiO2 on microstructure, microhardness, bioactivity, and biocompatibility of coatings was investigated. The experimental results illustrate that the coating doped with La2O3 and SiO2 has excellent metallurgical bonding. The XRD analysis confirms that the amount of hydroxyapatite and tricalcium phosphate in the coating reached maximum when doping amount of SiO2 is 10 wt %. SiO2‐doped coatings show a significantly higher bone‐like apatite precipitation after immersion in SBF than that of other coatings. in vitro experiment also shows that coating with 10 wt % SiO2 is more suitable for the attachment and proliferation of MG63 cells, indicating that coating with 10 wt % SiO2 exhibits best bioactivity and biocompatibility. These results suggest that the addition of SiO2 improves the bonding strength, bioactivity, and biocompatibility of coatings.
The diagnosis and prognosis values of WNT mRNA expression in colon adenocarcinoma
Authors: Ruan Guo‐Tian , Zhu Li‐Chen , Gong Yi‐Zhen , Liao Xi‐Wen , Wang Xiang‐Kun , Liao Cun , Wang Shuai , Yan Ling , Xie Hai‐Lun , Zhou Xin , Li Yang‐Zi , Gao Feng 
Published: December 2019

AbstractWNT family genes have participated in the progression and development of many cancers, however, the association between colon adenocarcinoma (COAD) and WNTs have been rarely reported. This study investigated the significance of WNT genes expression in COAD from the standpoint of diagnosis and prognosis. The RNA‐sequencing dataset of COAD was downloaded from The Cancer Genome Atlas and University of California, Santa Cruz Xena browser. The biology functions of WNT genes were investigated by biological analysis. Biological analysis of WNT family genes indicated that WNT genes were noticeably enriched in the complex process of WNT signaling pathway. The Pearson correlation analysis suggested WNT1 and WNT9B had a strong correlation. And receiver operating characteristic curves suggested that most of the genes could serve as a significant diagnostic makers in COAD (P < .05), especially WNT2 and WNT7B had high diagnostic values that the area under curve were 0.997 (95% confidence interval [0.994‐1.000]) and 0.961 (95%CI [0.939‐0.983]), respectively. And our multivariate survival analysis suggested the downregulated of WNT10B (P < .05) showed a favor prognosis in COAD overall survival. And the risk score model predicted that the upregulated expression of WNT10B might increase the risk of death. The very study we had conducted suggested that WNT genes had a certain connection with the diagnosis and prognosis of COAD. The messenger RNA expression of WNT2 and WNT7B might become potentially diagnostic biomarkers, and WNT10B might serve as an independent prognosis indicator for COAD.
Identification of hub‐methylated differentially expressed genes in patients with gestational diabetes mellitus by multi‐omic WGCNA basing epigenome‐wide and transcriptome‐wide profiling
Authors: Chen Min , Yan Jianying , Han Qing , Luo Jinying , Zhang Qinjian 
Published: December 2019

AbstractGestational diabetes mellitus (GDM), defined as dysglycaemia that is detected during pregnancy for the first time, has become a global health burden. GDM was found to be correlated to epigenetic changes, which would cause abnormal expression of placental genes. In the present study, we performed multi‐omic weighted gene coexpression network analysis (WGCNA) to systematically identify the hub genes for GDM using both epigenome‐ and transcriptome‐wide microarray data. Two microarray datasets (GSE70493 and GSE70494) were downloaded from the Gene Expression Omnibus (GEO) database. GEO2R was used to screen differentially expressed genes (DEGs) and differentially methylated genes (DMGs) between normal and GDM samples, separately. The results of WGCNA found that 15 modules were identified and the MEblack module had a significantly negative correlation with GDM (r = −.28, P = .03). GO enrichment analysis by BinGO of the MEblack module showed that genes were primarily enriched for the presentation of antigen processing, regulation of interferon‐α production and interferon‐γ‐mediated signaling pathway. By comparing the DEGs, DMGs and hub genes in the coexpression network, we identified five hypermethylated, lowly expressed genes (ABLIM1, GRHL1, HLA‐F, NDRG1, and SASH1) and one hypomethylated, highly expressed gene (EIF3F) as GDM‐related hub DMGs. Moreover, the expression levels of ABLIM1, GRHL1, HLA‐F, NDRG1, and SASH11 in the GDM patients and healthy controls were validated by a real‐time quantitative polymerase chain reaction. Finally, gene set enrichment analysis showed that the biological function of cardiac muscle contraction was enriched for four GDM‐related hub DMGs (ABLIM1, GRHL1, NDRG1, and SASH1). Analysis of this study revealed that dysmethylated hub genes in GDM placentas might affect the placental function and thus, take part in GDM pathogenesis and fetal cardiac development.
Noncoding RNA miR‐205‐5p mediates osteoporosis pathogenesis and osteoblast differentiation by regulating RUNX2
Authors: Huang Mingwei , Li Xiaowen , Zhou Can , Si Min , Zheng Haiyan , Chen Lianhua , Ding Hongcheng 
Published: December 2019

AbstractAs a kind of noncoding RNAs, microRNAs (miRNAs) play important roles in disease pathogenesis by regulating gene expression. However, the molecular mechanism of miRNAs in osteoporosis remains largely unknown. In the present study, we aim to explore the genome‐wide miRNAs expression profile and the regulatory mechanism of miR‐205‐5p in osteoporosis. A total of 72 differentially expressed miRNAs were identified in osteoporosis via microarray technology and bioinformatics analysis. We focused on one of the abnormally expressed miRNAs, miR‐205‐5p, which was previously unknown in osteoporosis. Quantitative real‐time polymerase chain reaction (qRT‐PCR) results showed that miR‐205‐5p was upregulated in osteoporosis samples and its expression was gradually decreased during osteogenic differentiation. Besides, miR‐205‐5p overexpression could inhibit the activity of osteoblast markers, including collagen, type I, α 1 (COL1A1) and alkaline phosphatase (ALP) while miR‐205‐5p inhibition showed the opposite results. Moreover, bioinformatics analysis identified the potential targets of miR‐205‐5p, including runt‐related transcription factor 2 (RUNX2), SMAD1 and BCL6, etc. The dual‐luciferase reporter assay confirmed RUNX2 was directly targeted by miR‐205‐5p. Furthermore, the rescue experiments showed that RUNX2 overexpression could significantly weaken the effect of miR‐205‐5p on osteoblast markers, indicating that miR‐205‐5p may inhibit osteogenic differentiation by targeting RUNX2.
Latinas’ knowledge of and experiences with genetic cancer risk assessment: Barriers and facilitators
Authors: Gómez‐Trillos Sara , Sheppard Vanessa B. , Graves Kristi D. , Song Minna , Anderson Lyndsay , Ostrove Nancy , Lopez Katherine , Campos Claudia , Gonzalez Nathaly , Hurtado‐de‐Mendoza Alejandra 
Published: December 2019

AbstractDisparities in genetic cancer risk assessment (GCRA) uptake persist between Latinas and Non‐Hispanic Whites. This study utilized a mental model approach to interview 20 Latinas (10 affected, 10 unaffected) at increased risk for hereditary breast and ovarian cancer (HBOC). Participants were asked about their knowledge and perceptions of GCRA, HBOC, risk, benefits, motivators, barriers, challenges, and experiences with GCRA. Using the Consensual Qualitative Analysis Framework, two authors independently coded the interviews and applied the final codes upon consensus. Additionally, interviews were coded to identify whether participants spontaneously brought up certain topics without a prompt. Findings identified multiple barriers and facilitators to GCRA uptake in this population, including patient level psychosocial/cultural factors (e.g., limited knowledge, worry about relatives’ risk) and healthcare system factors (e.g., receiving no referrals). There were notable differences in awareness and knowledge between affected and unaffected women (e.g., genetic testing awareness), as well as knowledge gaps that were evident in both groups (e.g., age of diagnosis as a risk factor). To reduce disparities in GCRA uptake, interventions should address identified facilitators and barriers. Differences in knowledge and awareness between affected and unaffected women support the development of targeted interventions that address specific knowledge gaps. This study was registered in ClinicalTrials.gov (NCT03075540) by Alejandra Hurtado de Mendoza, Ph.D.