bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sex differences in expression of immune elements emerge in children, young adults and mice with osteosarcoma
Authors: Mills Lauren J. , Spector Logan G. , Largaespada David A. , Williams Lindsay A. 
Published: January 2021

AbstractBackgroundMales < 40 years old are more likely to be diagnosed with and die from osteosarcoma (OS). The underlying mechanisms may depend on sex differences in immune response.MethodsWe used SEER data to estimate survival differences between males and females aged < 40 years at OS diagnosis. In NCI TARGET-OS cases, we determined sex differences in gene expression, conducted Gene Set Enrichment Analysis (GSEA), and applied the LM22 signature to identify biologic sex differences. We compared sex differences in gene expression profiles in TARGET-OS to those observed inSleeping Beauty(SB) transposon mutagenesis acceleratedTrp53R270H-mutant mouse-OS and healthy adult osteoblasts.ResultsMales had worse 17-year overall survival than females (SEERp< 0.0001). From 87 TARGET-OS cases, we observed 1018 genes and 69 pathways that differed significantly by sex (adjustedp< 0.05). Pathway and gene lists overlapped with those from mice (p= 0.03) and healthy osteoblasts (p= 0.017), respectively. Pathways that differed significantly by sex were largely immune-based and included the PD-1/PD-L1 immunotherapy pathway. We observed sex differences in M2 macrophages (LM22;p= 0.056) and M1-M2 macrophage transition (GSEA;p= 0.037) in TARGET-OS. LM22 trends were similar in mice. Twenty-four genes differentially expressed by sex in TARGET-OS had existing cancer therapies.ConclusionsSex differences in OS gene expression were similar across species and centered on immune pathways. Identified sex-specific therapeutic targets may improve outcomes in young individuals with OS.
JPEG image steganography payload location based on optimal estimation of cover co-frequency sub-image
Authors: Wang Jie , Yang Chunfang , Zhu Ma , Song Xiaofeng , Liu Yuan , Lian Yuemeng 
Published: January 2021

AbstractThe excellent cover estimation is very important to the payload location of JPEG image steganography. But it is still hard to exactly estimate the quantized DCT coefficients in cover JPEG image. Therefore, this paper proposes a JPEG image steganography payload location method based on optimal estimation of cover co-frequency sub-image, which estimates the cover JPEG image based on the Markov model of co-frequency sub-image. The proposed method combines the coefficients of the same position in each 8 × 8 block in the JPEG image to obtain 64 co-frequency sub-images and then uses the maximum a posterior (MAP) probability algorithm to find the optimal estimations of cover co-frequency sub-images by the Markov model. Then, the residual of each DCT coefficient is obtained by computing the absolute difference between it and the estimated cover version of it, and the average residual over coefficients in the same position of multiple stego images embedded along the same path is used to estimate the stego position. The experimental results show that the proposed payload location method can significantly improve the locating accuracy of the stego positions in low frequencies.
Impact of environmental cleaning on the colonization and infection rates of multidrug-resistant Acinetobacter baumannii in patients within the intensive care unit in a tertiary hospital
Authors: Li Yang , Ge Hai , Zhou Hui , Zhou Wanqing , Zheng Jie , Chen Wei , Cao Xiaoli 
Published: January 2021

Abstract
Objective
To continuously evaluate the effect of environmental cleaning and hand hygiene compliance on the colonization and infection rates of multidrug-resistant Acinetobacter baumannii (MDR-AB) in the patients within an intensive care unit (ICU).

Methods
Environmental cleaning on the high-touch clinical surfaces (HTCS) within a comprehensive ICU was evaluated through monitoring fluorescent marks when the overall compliance with hand hygiene during 2013–2014 was monitored. Meanwhile, samples from the HTCS and inpatients were collected and sent for bacterial culture and identification. The drug susceptibility testing was further implemented to monitor the prevalence of MDR-AB. The genetic relatedness of MDR-AB collected either from the HTCS or inpatients was analyzed by pulsed field gel electrophoresis (PFGE) when an outbreak was doubted.

Results
The overall compliance with hand hygiene remained relatively stable during 2013–2014. Under this circumstance, the clearance rate of fluorescence marks on the environmental surfaces within ICUs significantly increased from 21.9 to 85.7%, and accordingly the colonization and infection rates of MDR-AB decreased from 16.5 to 6.6‰ and from 7.4 to 2.8‰, respectively, from the beginning to the end of 2013. However, during 2014, because of frequent change and movement of environmental services staff, the clearance rate of fluorescence marks decreased below 50.0%, and the overall colonization and infection rates of MDR-AB correspondingly increased from 9.1 to 11.1‰ and from 1.5 to 3.9‰, respectively. PFGE displayed a high genetic relatedness between the MDR-AB strains analyzed, indicating a dissemination of MDR-AB during the surveillance period.

Conclusion
For the easily disseminated MDR-AB within ICUs, the clearance rate of fluorescence labeling on HTCS is negatively correlated with the hospital infection rate of MDR-AB. Such an invisible fluorescence labelling is an effective and convenient method to continuously monitor cleanness of medical environment within hospitals.
Impact of replacing powdered gloves with powder-free gloves on hand-hygiene compliance among healthcare workers of an intensive care unit: a quasi-experimental study
Authors: Menegueti Mayra Gonçalves , Bellissimo-Rodrigues Fernando , Ciol Marcia A. , Auxiliadora-Martins Maria , Basile-Filho Anibal , da Silva Canini Silvia Rita Marin , Gir Elucir , Laus Ana Maria 
Published: January 2021

Abstract
Background/objective
After wearing powdered gloves, healthcare workers (HCW) are supposed to wash their hands instead of using alcohol-based hand-rub (ABHR). Washing hands takes longer than using ABHR, and the use of powdered gloves may be an obstacle to hand-hygiene compliance. This study aimed to evaluate the impact of replacing powdered gloves with powder-free gloves on hand-hygiene compliance among HCW of an intensive care unit (ICU).

Methods
A quasi-experimental study was conducted in a general ICU of a tertiary care university hospital in Brazil. From June 1st to July 15th, 2017, all HCW were provided with powdered latex gloves only for all clinical procedures. From July 15th to August 31st, 2017, HCW were provided with nitrile powder-free gloves only. Hand-hygiene compliance was assessed through direct observation, and evaluated according to the World Health Organization Hand Hygiene guidelines. We calculated that a sample size of 544 hand hygiene opportunities needed to be observed per period. Data analysis were performed using the STATA SE® version 14, and we compared the individual’s percentage of compliance using the t test for paired data before and after the intervention.

Results
Overall, 40 HCW were assessed before and after the introduction of nitrile powder-free gloves, with 1114 and 1139 observations of hand hygiene opportunities, respectively. The proportion of compliance with hand hygiene was 55% (95% confidence interval [CI] 51–59%) using powdered latex gloves and 60% (95% CI 57–63%) using powder-free gloves. The difference in proportions between the two types of gloves was 5.1% (95% CI 2.5–7.6%, p < 0.001).

Conclusion
Our data indicate that replacing powdered gloves with powder-free gloves positively influenced hand-hygiene compliance by HCW in an ICU setting.
Bayesian zero-inflated regression model with application to under-five child mortality
Authors: Workie Mekuanint Simeneh , Azene Abebaw Gedef 
Published: January 2021

AbstractUnder-five mortality is defined as the likelihood of a child born alive to die between birth and fifth birthday. Mortality of under the age of five has been the most targets of public health policies and may be a common indicator of mortality levels. Thus, this study aimed to assess the under-five child mortality and modeling Bayesian zero-inflated regression model of the determinants of under-five child mortality. A community-based cross-sectional study was conducted using the 2016 Ethiopia Demographic and Health Survey data. The sample was stratified and selected in a two-stage cluster sampling design. The Bayesian analytic approach was applied to model the mixture arrangement inherent in zero-inflated count data by using the negative Binomial–logit hurdle model. About 71.09% of the mothers had not faced any under-five deaths in their lifetime while 28.91% of the women experienced the death of their under-five children and the data were found to have excess zeros. From Bayesian Negative Binomial—logit hurdle model it was found that twin (OR = 1.56; HPD CrI 1.23, 1.94), Primary and Secondary education (OR = 0.68; HPD CrI 0.59, 0.79), mother’s age at the first birth: 16–25 (OR = 0.83; HPD CrI 0.75, 0.92) and ≥ 26 (OR = 0.71; HPD CrI 0.52, 0.95), using contraceptive method (OR = 0.73; HPD CrI 0.64, 0.84) and antenatal visits during pregnancy (OR = 0.83; HPD CrI 0.75, 0.92) were statistically associated with the number of non-zero under-five deaths in Ethiopia. The finding from the Bayesian Negative Binomial–logit hurdle model is getting popular in data analysis than the Negative Binomial–logit hurdle model because the technique is more robust and precise. Furthermore, Using the Bayesian Negative Binomial–logit hurdle model helps in selecting the most significant factor: mother’s education, Mothers age, Birth order, type of birth, mother’s age at the first birth, using a contraceptive method, and antenatal visits during pregnancy were the most important determinants of under-five child mortality.
Antitumor Effect of 5-Fluorouracil-Loaded Liposomes Containing n-3 Polyunsaturated Fatty Acids in Two Different Colorectal Cancer Cell Lines
Authors: Dupertuis Yves Marc , Boulens Nathalie , Angibaud Emmanuelle , Briod Anna-Sophia , Viglione Alexandre , Allémann Eric , Delie Florence , Pichard Claude 
Published: January 2021

AbstractIt has been shown that long-chain n-3 polyunsaturated fatty acids (n-3 PUFAs) could act synergistically with 5-fluorouracil (5-FU) to kill cancer cells. To facilitate their simultaneous transport in the bloodstream, we synthesized, for the first time, liposomes (LIPUFU) containing 5-FU in the aqueous core and docosahexaenoic acid (DHA)/eicosapentaenoic acid (EPA) at a ratio of 1:2 in the lipid bilayer. LIPUFU werestable with uniform size of 154 ± 4 nm, PDI of 0.19 ± 0.03 and zeta potential of -41 ± 2 mV. They contained 557 ± 210 μmol/l DHA, 1467 ± 362 μmol/l EPA, and 9.8 ± 1.1 μmol/l 5-FU. Control liposomes without (LIP) or with only 5-FU (LIFU) or n-3 PUFAs (LIPU) were produced in a similar way. The effects of these different liposomal formulations on the cell cycle, growth, and apoptosis were evaluated in two human colorectal cancer (CRC) cell lines differing in sensitivity to 5-FU, using fluorescence-activated cell sorting analyses. LIPUFU were more cytotoxic than LIP, LIFU, and LIPU in both LS174T (p53+/+, bax−/−) and HT-29 (p53−/0, bax+/+) cell lines. Similar to LIFU, LIPUFU increased the percentage of cells in S phase, apoptosis, and/or necrosis. The cytotoxic potential of LIPUFU was confirmed in vivo by tumor growth inhibition in the chicken chorioallantoic membrane model. These results suggest that LIPUFU could be considered to facilitate the simultaneous transport of 5-FU and n-3 PUFAs to the tumor site, in particular in case of CRC liver metastases.
Shot noise as a probe for the pairing symmetry of iron pnictide superconductors
Authors: Benjamin Colin , Mohapatra Tusaradri 
Published: January 2021

Abstract
One of the outstanding problems in Iron pnictide research is the unambiguous detection of its pairing symmetry. The most probable candidates are the two-band

and sign-reversed

wave pairing. In this work the Andreev conductance and shot noise are used as a probe for the pairing symmetry of Iron pnictide superconductors. Clear differences emerge in both the zero bias differential conductance and the shot noise in the tunneling limit for the two cases enabling an effective distinction between the two.
Coherent propagation of powerful out-of-phase wave beams in linear arrays of weakly coupled cores
Authors: Balakin A. A. , Skobelev S. A. , Litvak A. G. 
Published: January 2021

Abstract
The coherent propagation and amplification of high-power laser beams in a multicore fiber (MCF) of

and

lattices of weakly coupled cores is studied. Exact stable analytical nonlinear solutions are found in the form of the out-of-phase distribution that describe the coherent propagation of wave beams in such MCFs. The use of these distributions makes it possible to operate with laser radiation with a power of up to 2N times of the critical self-focusing power in homogeneous media. The analytical results are confirmed by the direct numerical simulation of the wave equation. The limitations on active fiber parameters for stable amplification of the found out-of-phase solution are determined.
Poly(ADP-ribosyl)ation temporally confines SUMO-dependent ataxin-3 recruitment to control DNA double-strand break repair
Authors: Pfeiffer Annika , Herzog Laura K. , Luijsterburg Martijn S. , Shah Rashmi G. , Rother Magdalena B. , Stoy Henriette , Kühbacher Ulrike , van Attikum Haico , Shah Girish M. , Dantuma Nico P. 
Published: January 2021

ABSTRACT
DNA damage-induced SUMOylation serves as a signal for two antagonizing proteins that both stimulate repair of DNA double-strand breaks (DSBs). Here, we demonstrate that the SUMO-dependent recruitment of the deubiquitylating enzyme ataxin-3 to DSBs, unlike recruitment of the ubiquitin ligase RNF4, additionally depends on poly [ADP-ribose] polymerase 1 (PARP1)-mediated poly(ADP-ribosyl)ation (PARylation). The co-dependence of ataxin-3 recruitment on PARylation and SUMOylation temporally confines ataxin-3 to DSBs immediately after occurrence of DNA damage. We propose that this mechanism ensures that ataxin-3 prevents the premature removal of DNA repair proteins only during the early phase of the DSB response and does not interfere with the subsequent timely displacement of DNA repair proteins by RNF4. Thus, our data show that PARylation differentially regulates SUMO-dependent recruitment of ataxin-3 and RNF4 to DSBs, explaining how both proteins can play a stimulatory role at DSBs despite their opposing activities.
A novel degree of sex difference in laryngeal physiology of Xenopus muelleri: behavioral and evolutionary implications
Authors: South Kelly E. , Klingenberg Bernhard , Leininger Elizabeth C. 
Published: January 2021

ABSTRACT
Characterizing sex and species differences in muscle physiology can contribute to a better understanding of proximate mechanisms underlying behavioral evolution. In Xenopus, the laryngeal muscle's ability to contract rapidly and its electromyogram potentiation allows males to produce calls that are more rapid and intensity-modulated than female calls. Prior comparative studies have shown that some species lacking typical male features of vocalizations sometimes show reduced sex differences in underlying laryngeal physiology. To further understand the evolution of sexually differentiated laryngeal muscle physiology and its role in generating behavior, we investigated sex differences in the laryngeal physiology of X. muelleri, a species in which male and female calls are similar in rapidity but different with respect to intensity modulation. We delivered ethologically relevant stimulus patterns to ex vivo X. muelleri larynges to investigate their ability to produce various call patterns, and we also delivered stimuli over a broader range of intervals to assess sex differences in muscle tension and electromyogram potentiation. We found a small but statistically significant sex difference in laryngeal electromyogram potentiation that varied depending on the number of stimuli. We also found a small interaction between sex and stimulus interval on muscle tension over an ethologically relevant range of stimulus intervals; male larynges were able to produce similar tensions to female larynges at slightly smaller (11–12 ms) inter-stimulus intervals. These findings are consistent with behavioral observations and present a previously undescribed intermediate sex difference in Xenopus laryngeal muscle physiology.
R&D approach based on multiple partners and Design Thinking, Lean Startup, and Agile concepts: A case study in the electricity sector
Authors: Leal Lorenna Fernandes , Ribeiro Artur Tavares Vilas Boas , Romão Victor , Do Amaral Guilherme Soares Gurgel , Altmann Ricardo , Kahn Ricardo , Pacci Bruno Guilherme , Avo Marcos , Salerno Mario Sergio , Plonski Guilherme Ary , Zancul Eduardo 
Published: January 2021

Goal: The paper aims to increase the understanding of the results and challenges that arise when running R&D projects based on multiple partners and the integration of Design Thinking, Lean Startup, and Agile concepts.
Design / Methodology / Approach: We followed a case study approach in the electricity sector. Two project cases were studied, using triangulated data from interviews, internal documents, and non-participant observations.
Results: The results of the Design Thinking, Lean Startup, and Agile concepts application in R&D projects show improvements in knowledge absorption, solution improvement through collaborative insights between partners, and identification of short-term opportunities. Some challenges, however, were identified, such as noisy integration, the role of the project manager requiring a sophisticated skillset, and complicated decision-making processes.
Limitations of the investigation: The conclusions have the inherent limitations of a case study conducted in one industrial sector and two projects led by one company.
Practical implications: The evolving competitive landscape demands more effective pathways to foster innovation. Recent approaches to innovation have challenged R&D practices. This study discusses a new way of addressing R&D efforts with multiple partners by integrating Design Thinking, Lean Startup, and Agile concepts.
Originality / Value: The combined and coherent application of concepts derived from Design Thinking, Lean Startup, and Agile in R&D practices in the electricity sector is new. The high volume of R&D activity in the electricity sector makes this in-depth analysis both relevant and valuable.
Assessment of Patient Satisfaction Among Pregnant Patients in The Out-Patient Department of Obstetrics and Gynecology Section at Metropolitan Medical Center Manila
Authors: Widjaja Herry Prabowo Krestiyanto 
Published: January 2021

ABSTRACT Patient satisfaction reflects the quality of an institution’s delivery of health care services. Patient satisfaction is a critical factor in improvement or complete recuperation to wellness because satisfied patients will mean better compliance to treatment or follow up. As reported in the 2017 Annual OPD census of the Department of Obstetrics and Gynecology, there were 2617 total patient consultations. Majority of these (2138 or 81.7 %) were obstetrical cases. The average number of consultations per day (for Obstetrics and Gynecology patients combined) is 91. Therefore, the Out-Patient Clinic plays an important role in prenatal care specifically the prevention of adverse maternal and neonatal morbidity and mortality. This study aimed to assess the level of patient satisfaction among obstetrical patients in the Out Patient Department of the Obstetrics and Gynecology Section at Metropolitan Medical Center from July 1, 2018 to July 31, 2018 and to see the relationship between socio-demographic characteristic and the level of patient satisfaction. This is a descriptive, cross sectional study. The level of patient satisfaction assessed as to physical facilities, interaction between doctor and patient, interaction between nurses and patient, and registration service. There were sixty three subjects in this study. Sixty three were satisfied with inclusion criteria. The validated questionnaire was the pretested questionnaire which was 96 % valid and reliable by Cronbach’s analysis. Statistical analysis was done which showed respondents were generally satisfied. There were significant relationship association between socio-demographic characteristics and level of patient satisfaction with P value < 0.05.Key Word : Patient Satisfaction, Socio Demographic Characteristic
„Koronapló”
Authors: Bakos Lili , Csuvik Mariann , Farkas Anett , Graffits Eszter , Hegedűs Lilla Csenge , Horváth Patrik , Józsa Ferenc , Kaiser Kornél , Keszthelyi Boglárka Enikő , Nagy Eszter , Nánai Tamás , Sándori Enikő , Vidovics Vanessza , Brettner Zsuzsanna 
Published: January 2021

A koronavírus 2020 eleje óta mindenki számára a hétköznapok részévé vált. A reflektív naplók részletei alapján elmondható, hogy az egyetemi hallgatók élete is gyökeresen, egyik pillanatról a másikra megváltozott. Sokuknak haza kellett költöznie, amely újraformálta a korábbi családi kapcsolatokat, a vírus közvetlenül bontotta meg a család addig jól működő dinamikáját. Legtöbbjük ez idő alatt nem tudott dolgozni, néhányuk családjában a szülők is elvesztették munkájukat. Az idősebb rokonokkal legtöbben nem érintkezhettek, barátaikkal sem találkoztak, saját mindennapi rutinjuk is felborult, különösen az online, távolléti oktatásnak köszönhetően. Ezeket a nehézségeket több módon igyekeztek feldolgozni. Az évfolyamtársaik közül ketten külföldön élték meg a járványt. Volt, aki önkéntes munkát vállalt, de a legtöbben a négy fal között vészelték át ezt az időszakot. Összességében elmondható, hogy nem létezett olyan élettér, amelyre ne lett volna hatással a járvány. A szerzők mint szociális munkás hallgatók életük azon területeiről írnak, amelyekben a változást a legjelentősebbnek ítélték meg.
Galactosemia: Revisión de la literatura
Authors: Godoy Salgado Celenia , Sabillon Mendoza Alejandra , Zarate Mondragon Flora , Toro Monjaraz Erick , Cadena Leon Jose , Ignorosa Arellano Karen , Loredo Mayer Alejandro , Cervantes Bustamante Roberto , Ramirez Mayans Jaime 
Published: January 2021

La galactosemia es un trastorno hereditario del metabolismo de la galactosa causado por una actividad deficiente en una de las cuatro enzimas de la vía de Leloir. La forma clásica y severa de presentación es la deficiencia de Galactosa-1-fostato uridiltransferasa (GALT) la cual se manifiesta prácticamente desde el periodo neonatal como una enfermedad potencialmente mortal y cuyo cuadro clínico puede resolverse con la restricción de la galactosa de la dieta; sin embargo, en algunos casos el tratamiento ha demostrado ser insuficiente para prevenir complicaciones a largo plazo tales como deficiencias cognitivas, sociales y reproductivas. El objetivo del presente artículo, es realizar descripción detallada de la galactosemia, sus variantes clínicas y bioquímicas, así como diagnóstico y manejo de manera que el medico pediatra este familiarizado con esta enfermedad, tenga un alto índice de sospecha y pueda realizar una detección temprana.