bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cell Therapy With Mesenchymal Stem Cells Induces an Innate Immune Memory Response That Attenuates Experimental Colitis in the Long Term
Authors: Lopez-Santalla Mercedes , Hervas-Salcedo Rosario , Fernandez-Garcia Maria , Bueren Juan Antonio , Garin Marina Inmaculada 
Published: April 2020

Abstract

Background and Aims
Mesenchymal stem cells [MSCs] are used in preclinical and clinical studies for treatment of immune-mediated disorders, thanks to their immunomodulatory properties. Cell therapy with MSCs induces multiple effects in the immune system which ultimately lead to increase in the number of immune cells with regulatory phenotype. In this study, we investigated whether the beneficial effects of MSC therapy are maintained in the long term in a clinically relevant mouse model of colitis.

Methods
A single dose of adipose-derived MSCs [aMSCs] was infused into dextran sulphate sodium [DSS]-induced colitic mice during the induction phase of the disease. Following a latency period of 12 weeks, mice were re-challenged with a second 7-day cycle of DSS.

Results
DSS-induced colitic mice treated with aMSCs showed significant reduction in their colitic disease activity index during the second DSS challenge when compared with non-aMSC treated DSS-induced colitic mice. Strikingly, the long-term protection induced by aMSC therapy was also observed in Rag-1-/- mice where no adaptive immune memory cell responses take place. Increased percentages of Ly6G+CD11b+ myeloid cells were observed 12 weeks after the first inflammatory challenge in the peritoneal cavity, spleen, and bone marrow of DSS-induced colitic mice that were infused with aMSCs. Interestingly, upon re-challenge with DSS, these animals showed a concomitant increase in the regulatory/inflammatory macrophage ratio in the colon lamina propria.

Conclusions
Our findings demonstrate for the first time that MSC therapy can imprint an innate immune memory-like response in mice which confers sustained protection against acute inflammation in the long term.
Cannabis-induced recurrent myocardial infarction in a 21-year-old man: a case report
Authors: Lawin Dennis , Lawrenz Thorsten , Tego Andi , Stellbrink Christoph 
Published: April 2020

Abstract

Background
Acute coronary syndrome (ACS) is rarely caused by coronary artery disease in young patients unless cardiovascular risk factors are present. Although non-atherosclerotic causes of ACS are rare, they need to be considered in young patients.

Case summary
We report on a 21-year-old patient referred to our institution with ACS. Electrocardiogram showed ST-segment elevation and coronary angiography revealed thrombotic occlusion of the left anterior descending artery. Reperfusion was achieved by thrombus aspiration, glycoprotein IIb/IIIa inhibitors (GPI), and drug-eluting stent (DES). The patient had no cardiovascular risk factors but reported cannabis consumption before symptom onset. Although he was put on dual antiplatelet therapy and strictly advised to avoid consumption, he continued to abuse cannabis and suffered three further ACS events within 18 months: the first 8 months later caused by thrombotic occlusion of a diagonal branch treated by GPI and DES, the second after 17 months due to thrombotic re-occlusion of the diagonal branch, and the third after 18 months by thrombotic occlusion of the circumflex artery, both events treated by GPI alone (all while still using cannabis). Since then, he stopped cannabis consumption and has been symptom-free for 8 months.

Discussion
This case highlights that cannabis-induced ACS must be considered as a cause of myocardial infarction in young adults. In contrast to ACS in the elderly population, this unusual ACS cause requires specific treatment. The risk of ACS relapse may substantial if cannabis abuse is continued. This potential hazard needs to be taken into consideration when legalization of cannabis is discussed.
Single-shot cryoablation of an epicardial inferoparaseptal accessory pathway: a case report
Authors: Davtyan Karapet V , Topchyan Arpi H , Kalemberg Andrey A , Simonyan Georgiy Yu 
Published: April 2020

Abstract

Background
Catheter ablation (CA) of epicardial accessory pathways (APs) can be performed via the coronary sinus (CS) system. Variable CS anatomy with complications of former CA procedures inside the CS venous system may require using alternative CA approach and technology.

Case summary
We report the case of a 23-year-old man with Wolff–Parkinson–White syndrome and history of aborted sudden cardiac death (SCD) and unsuccessful previous AP radiofrequency ablation (RFA). CS venography during the redo procedure revealed an early CS trifurcation with cardiac veins stenosis, thus with difficulties in maintaining cardiac veins’ access and catheter manoeuvring inside CS venous system. The last office visit with electrocardiogram (ECG) performance was in 3 months after the CA. Neither delta wave on the ECG nor any complaints/adverse health effects was detected at that time.

Discussion
Successful CA of epicardial AP in patients with a high risk of SCD is essential. However, CS complex anatomy and changes after former RFA inside it may lead to CS venous system access limitations. Alternative CA approach and technology should be considered to ensure CS venous system cannulation and epicardial AP CA performance.
Abundance, distribution, and habitat use of fishes in a large river (Danube, Austria): mobile, horizontal hydroacoustic surveys vs. a standard fishing method
Authors: Kerschbaumer Philip , Tritthart Michael , Keckeis Hubert 
Published: April 2020

Abstract
We tested a novel approach for fish abundance and size estimates along a 3.6-km reach in the main stem of a free-flowing section of the Danube River east of Vienna. We deployed an adaptive resolution imaging sonar (ARIS) with horizontal beam orientation from a moving boat in order to assess spatial and temporal fish abundance distributions in different mesohabitats. To validate the results obtained from imaging sonar, hydroacoustic abundance and fish length estimates were compared with those received from boat electrofishing surveys conducted at the same time and place. Furthermore, fish occurrence was related to hydraulic conditions based on a three-dimensional hydrodynamic model in order to reveal patterns of habitat use. Hydroacoustic abundance expressed as catch per unit effort estimates deviated significantly from fish abundances assessed by boat electrofishing, but when comparing relative abundances both methods yielded a similar spatial and temporal pattern. Total fish length estimates obtained from the two methods did not differ significantly. The mobile application of the non-invasive ARIS technology offers the opportunity to quickly gain detailed information on fish abundance and position of individuals. This yields more specific information on temporal and spatial distribution patterns, habitat use and fish size distributions in large rivers.
Scalability and cost-effectiveness analysis of whole genome-wide association studies on Google Cloud Platform and Amazon Web Services
Authors: Krissaane Inès , De Niz Carlos , Gutiérrez-Sacristán Alba , Korodi Gabor , Ede Nneka , Kumar Ranjay , Lyons Jessica , Manrai Arjun , Patel Chirag , Kohane Isaac , Avillach Paul 
Published: April 2020

Abstract

Objective
Advancements in human genomics have generated a surge of available data, fueling the growth and accessibility of databases for more comprehensive, in-depth genetic studies.

Methods
We provide a straightforward and innovative methodology to optimize cloud configuration in order to conduct genome-wide association studies. We utilized Spark clusters on both Google Cloud Platform and Amazon Web Services, as well as Hail (http://doi.org/10.5281/zenodo.2646680) for analysis and exploration of genomic variants dataset.

Results
Comparative evaluation of numerous cloud-based cluster configurations demonstrate a successful and unprecedented compromise between speed and cost for performing genome-wide association studies on 4 distinct whole-genome sequencing datasets. Results are consistent across the 2 cloud providers and could be highly useful for accelerating research in genetics.

Conclusions
We present a timely piece for one of the most frequently asked questions when moving to the cloud: what is the trade-off between speed and cost?
The N-terminal amino-latch region of Hlg2 component of staphylococcal bi-component γ-haemolysin is dispensable for prestem release to form β-barrel pores
Authors: Takeda Kein , Tanaka Yoshikazu , Kaneko Jun 
Published: April 2020

Abstract
The contribution of N-terminal regions of staphylococcal bi-component γ-haemolysin toxin components to haemolytic activity towards human erythrocyte cells was investigated in this study. A deletion construct of N-terminal amino acids 1–10 of Hlg2 (Hlg2 ΔN10), which is the S-component protein of γ-haemolysin, had little effect on its haemolytic activity, whereas N-terminal 1–11 amino acid deletion (Hlg2 ΔN11) significantly delayed haemolysis. Moreover, a deletion of N-terminal amino acids 1–17 of LukF, which is the F-component protein of γ-haemolysin, increased its haemolytic activity in combination with either the wild-type or Hlg2 ΔN10. Unlike the N-terminal amino-latch region of staphylococcal α-haemolysin, which is a single component β-barrel pore-forming toxin, the N-terminal regions present in γ-haemolysin components are dispensable for the haemolytic activity of the bi-component toxin. These results strengthen our recent proposal that staphylococcal bi-component γ-haemolysin toxin uses an N-terminal amino-latch independent molecular switch for prestem release during the formation of β-barrel pores.
The Multiple Stories Behind the Modern Ceramic Coffee Tables of the Grand National Assembly of Turkey
Authors: Hasirci Deniz , Tuna Ultav Zeynep 
Published: April 2020

Abstract
At the end of the 1950s, 200 coffee tables and other furniture and fittings were created by interior designers who won a design competition organized by the Grand National Assembly of Turkey (GNAT) in Ankara. The tables were envisioned to be made by modern ceramic artists and designers of the time. Despite never meeting, they created joint works that were celebrated at the time for their artistic quality. The coffee tables, which were used by government ministers, were useful for introducing ceramics into government interiors while informing the leaders of a modernizing Turkish nation about modern art. This study traces the stories behind the creation of these unique artworks based on the personal stories of Füreya Koral, Sadun Ersin, and the Taylan Ceramics Atelier, who were all significant contributors to the history of this furniture. The method used included literature review, a comprehensive archival research through popular magazines, and interviews with artists and designers of the time. The interviews helped uncover data that could not be accessed otherwise. The findings contribute to Turkish and international design history, uncovering artworks and identifying modernist artists and designers of the time in Turkey.
Sherlock at the ICC?
Authors: Braga da Silva Rafael 
Published: April 2020

Abstract
The International Criminal Court (ICC or the Court) is currently suffering from an evidence problem. Since the time the ICC became functional, some judges have criticized the investigative practices of the Office of the Prosecutor (OTP), and some cases have crumbled due to the quality of evidence. This article tackles these problems from the perspective of third-party investigations and their role in ICC cases. It examines the difference between private and public investigators under the legal framework of the Rome Statute to find whether and how third-party investigations can be used in (and by) the ICC. Evidence collected by third-party investigators will likely face challenges of admissibility in being introduced into trial. Those challenges could, however, be overcome if third-party investigations are regulated within the legal framework of the ICC. Ultimately, this article aims to provide a legal framework to optimize the use of third-party investigations in the ICC.
Delayed presentation of a traumatic descending thoracic aorta aneurysm as an aorto-oesophageal fistula
Authors: Mohamed Saifullah , Patel Akshay J , Iqbal Yassir H , Ranasinghe Aaron 
Published: April 2020

Abstract
Aorto-oesophageal fistula is a rarely described but life-threatening complication presenting with upper gastrointestinal haemorrhage. We present a case of a 58-year-old gentleman who presented to a peripheral hospital with a short duration of haematemesis and melaena and recent onset symptoms of dysphagia. This gentleman had a past medical history of alcohol excess and a history of trauma following a road traffic accident 38 years ago. Initial computer tomography scan demonstrated a large saccular aneurysm arising from the descending thoracic aorta adjacent to the oesophagus. The patient underwent radiological stenting of the large aneurysm with thoracic endovascular aortic replacement procedure. Follow-up computerized tomography (CT) angiography demonstrated satisfactory appearances of the thoracic aortic stent graft with no evidence of endo-leak. The triad of dysphagia, occult gastrointestinal blood loss and CT findings of a large aneurysm should prompt diagnosis or aorto-oesophageal fistula until proven otherwise.
The Indian Doctress in the Nineteenth-Century United States: Race, Medicine, and Labor
Authors: Hudson Angela Pulley 
Published: April 2020

Abstract
This article addresses the robust market in “Indian medicine” that flourished in the nineteenth century—partly due to the influence of urbanization, industrialization, and new technologies of print—and the specific roles that Indian doctresses played in that phenomenon. Indian doctresses in the United States operated at the intersection of cultural values and beliefs regarding womanhood, medicine, and American Indians. Not all of these women were of Native ancestry, but they all mobilized widespread ideas about Native peoples while seeking entrepreneurial success as healers. Using print culture, the author analyzes strategies employed by women who worked as Indian doctresses and patterns of reactions to their efforts. By combining profiles of women who worked as Indian doctresses with popular but not always positive representations of the type, the article offers a kind of composite biography of an occupation. Women from a wide variety of backgrounds fused caregiving skills with popular assumptions—particularly those involving “indigenous anti-modernity”—to make a living. In this way, Indian doctresses also became useful symbolic figures upon whom changing conceptions of race, gender, and class could be projected and debated. The author thus aims to shed new light not only on histories of American medicine but also on the labors of American women and the business of Indian representation during the nineteenth century.
The CLE53–SUNN genetic pathway negatively regulates arbuscular mycorrhiza root colonization in Medicago truncatula
Authors: Karlo Magda , Boschiero Clarissa , Landerslev Katrine Gram , Blanco Gonzalo Sancho , Wen Jiangqi , Mysore Kirankumar S , Dai Xinbin , Zhao Patrick X , de Bang Thomas C 
Published: April 2020

Abstract
Plants and arbuscular mycorrhizal fungi (AMF) engage in mutually beneficial symbioses based on a reciprocal exchange of nutrients. The beneficial character of the symbiosis is maintained through a mechanism called autoregulation of mycorrhization (AOM). AOM includes root-to-shoot-to-root signaling; however, the molecular details of AOM are poorly understood. AOM shares many features of autoregulation of nodulation (AON) where several genes are known, including the receptor-like kinase SUPER NUMERIC NODULES (SUNN), root-to-shoot mobile CLAVATA3/ENDOSPERM SURROUNDING REGION (ESR)-RELATED (CLE) peptides, and the hydroxyproline O-arabinosyltransferase ROOT DETERMINED NODULATION1 (RDN1) required for post-translational peptide modification. In this work, CLE53 was identified to negatively regulate AMF symbiosis in a SUNN- and RDN1-dependent manner. CLE53 expression was repressed at low phosphorus, while it was induced by AMF colonization and high phosphorus. CLE53 overexpression reduced AMF colonization in a SUNN- and RDN1 dependent manner, while cle53, rdn1, and sunn mutants were more colonized than the wild type. RNA-sequencing identified 700 genes with SUNN-dependent regulation in AMF-colonized plants, providing a resource for future identification of additional AOM genes. Disruption of AOM genes in crops potentially constitutes a novel route for improving AMF-derived phosphorus uptake in agricultural systems with high phosphorus levels.
Performance characteristics of Fungitell STAT™, a rapid (1→3)-β-D-glucan single patient sample in vitro diagnostic assay
Authors: D'Ordine Robert L , Garcia Kevin A , Roy Josee , Zhang Yonglong , Markley Barbara , Finkelman Malcolm A 
Published: April 2020

Abstract
Serum (1→3)-β-D-glucan (BDG), is an adjunct test in the diagnosis of invasive fungal disease (IFD). Fungitell STAT™, a facile, rapid, single patient option, executable for one or more patient specimens in approximately an hour, has been developed to address a need for rapid in-house testing. This method presents qualitative information concerning serum BDG levels, using an index value that allows the rapid categorization of patients as positive, negative, or indeterminate relative to serum BDG titer. The categorical and analytical performance of Fungitell STAT was evaluated. The categorical agreement between methods was established by testing patient samples which had been previously categorized with Fungitell. Receiver Operating Characteristic curves were used to identify cut-offs using 93 de-identified patient specimens. Subsequently, using these cutoffs, an independent group of 488 patient specimens was analyzed. Positive percent agreement (PPA) with, and without, indeterminate results was 74% and 99%, respectively. Negative percent agreement (NPA) was 91% and 98% with, and without, indeterminate results, respectively. Additionally, commercially available normal off-the-clot sera were spiked with Saccharomyces cerevisiae-derived (1→3)-β-D-glucan to produce analytical samples. Analytical reproducibility using spiked samples was excellent with 94% of the CV (coefficient of variation) values ≤10% among three independent laboratories. Good correlation with the predicate method was demonstrated with correlation coefficients of 0.90 or better with patient samples and 0.99 with spiked samples. The Fungitell STAT index assay provides a rapid and suitable method for serum BDG testing.
Effect of bars on evolution of SDSS spiral galaxies
Authors: Kim Minbae , Choi Yun-Young , Kim Sungsoo S 
Published: April 2020

ABSTRACT
We explore the significance of bars in triggering central star formation (SF) and active galactic nucleus (AGN) activity for spiral galaxy evolution using a volume-limited sample with 0.020 &lt; z &lt; 0.055, Mr &lt; −19.5, and σ &gt; 70 km s−1 selected from Sloan Digital Sky Survey Data Release 7. On a central SF rate–σ plane, we measure the fraction of galaxies with strong bars in our sample and also the AGN fractions for barred and non-barred galaxies, respectively. The comparison between the bar and AGN fractions reveals a causal connection between the two phenomena of SF quenching and AGN activity. A massive black hole and abundant gas fuels are sufficient conditions to trigger AGNs. We infer that the AGNs triggered by satisfying the two conditions drive the strong AGN feedback, suddenly suppressing the central SF and leaving the SF sequence. We find that in galaxies where either of the two conditions is not sufficient, bars are a great help for the AGN triggering, accelerating the entire process of evolution, which is particularly evident in pseudo-bulge galaxies. All of our findings are obtained only when plotted in terms of their central velocity dispersion and central SFR (not galactic scale SFR), indicating that the AGN-driven SF quenching is confined in the central kpc region.
Not all stars form in clusters – Gaia-DR2 uncovers the origin of OB associations
Authors: Ward Jacob L , Kruijssen J M Diederik , Rix Hans-Walter 
Published: April 2020

ABSTRACT
Historically, it has often been asserted that most stars form in compact clusters. In this scenario, present-day gravitationally unbound OB associations are the result of the expansion of initially gravitationally bound star clusters. However, this paradigm is inconsistent with recent results, both theoretical and observational, that instead favour a hierarchical picture of star formation in which stars are formed across a continuous distribution of gas densities and most OB associations never were bound clusters. Instead they are formed in situ as the low-density side of this distribution, rather than as the remnants of expanding clusters. We utilize the second Gaia data release to quantify the degree to which OB associations are undergoing expansion and, therefore, whether OB associations are the product of expanding clusters, or whether they were born in situ, as the large-scale globally unbound associations that we see today. We find that the observed kinematic properties of associations are consistent with highly substructured velocity fields and additionally require some degree of localized expansion from subclusters within the association. While most present-day OB associations do exhibit low levels of expansion, there is no significant correlation between radial velocity and radius. Therefore, the large-scale structure of associations is not set by the expansion of clusters, rather it is a relic of the molecular gas cloud from which the association was formed. This finding is inconsistent with a monolithic model of association formation and instead favours a hierarchical model, in which OB associations form in situ, following the fractal structure of the gas from which they form.
SPEED2: inferring upstream pathway activity from differential gene expression
Authors: Rydenfelt Mattias , Klinger Bertram , Klünemann Martina , Blüthgen Nils 
Published: April 2020

Abstract
Extracting signalling pathway activities from transcriptome data is important to infer mechanistic origins of transcriptomic dysregulation, for example in disease. A popular method to do so is by enrichment analysis of signature genes in e.g. differentially regulated genes. Previously, we derived signatures for signalling pathways by integrating public perturbation transcriptome data and generated a signature database called SPEED (Signalling Pathway Enrichment using Experimental Datasets), for which we here present a substantial upgrade as SPEED2. This web server hosts consensus signatures for 16 signalling pathways that are derived from a large number of transcriptomic signalling perturbation experiments. When providing a gene list of e.g. differentially expressed genes, the web server allows to infer signalling pathways that likely caused these genes to be deregulated. In addition to signature lists, we derive ‘continuous’ gene signatures, in a transparent and automated fashion without any fine-tuning, and describe a new algorithm to score these signatures.