bluuebunny/crossref_metadata_2025_split huggingface dataset:
Immunosuppressive properties of CD95L-transduced “killer” hybrids created by fusing donor- and recipient-derived dendritic cells
Authors: Matsue Hiroyuki , Matsue Keiko , Kusuhara Masahiro , Kumamoto Tadashi , Okumura Ko , Yagita Hideo , Takashima Akira 
Published: July 2002

AbstractAllogeneic immune responses, which are initiated by dendritic cells (DCs) of both donor and host origins, remain a major obstacle in organ transplantation. Presentation of intact major histocompatibility complex (MHC) molecules by allogeneic DCs and allogeneic peptides by syngeneic DCs leads to complex allogeneic immune responses. This study reports a novel strategy designed to suppress both pathways. A stable DC line XS106 (A/J mouse origin) was transfected with CD95L cDNA and fused with splenic DCs purified from allogeneic BALB/c mice. The resulting “killer” DC-DC hybrids: (1) expressed CD95L and MHC class I and class II molecules of both A/J and BALB/c origins, while maintaining otherwise characteristic surface phenotypes of mature DCs; (2) inhibited MHC class I– and class II–restricted mixed leukocyte reactions between the parental strains by triggering apoptosis of alloreactive T cells; and (3) abolished delayed-type hypersensitivity responses of A/J (and BALB/c) mice to BALB/c-associated (and A/J-associated) alloantigens when injected intravenously into A/J (and BALB/c) mice. The onset of graft-versus-host disease in (BALB/c × A/J) F1 hosts receiving A/J-derived hematopoietic cell transplantation was suppressed significantly (P &lt; .001) by killer DC-DC hybrid treatment. These results form both technical and conceptual frameworks for clinical applications of CD95L-transduced killer hybrids created between donor DCs and recipient DCs in the prevention of allogeneic immune responses following organ transplantation.
Chemoattractants MDC and TARC are secreted by malignant B-cell precursors following CD40 ligation and support the migration of leukemia-specific T cells
Authors: Ghia Paolo , Transidico Pietro , Veiga J. Pedro , Schaniel Christoph , Sallusto Federica , Matsushima Kouji , Sallan Stephen E. , Rolink Antonius G. , Mantovani Alberto , Nadler Lee M. , Cardoso Angelo A. 
Published: July 2002

AbstractThe use of tumor cells as vaccines in cancer immunotherapy is critically dependent on their capacity to initiate and amplify tumor-specific immunity. Optimal responses may require the modification of the tumor cells not only to increase their immunogenicity but also to improve their ability to recruit effector cells to the tumor sites or sites of tumor antigen exposure. It has been reported that CD40 cross-linking of acute lymphoblastic leukemia (ALL) cells significantly increases their immunogenicity and allows the generation and expansion of autologous antileukemia cytotoxic T lymphocytes. This study demonstrates that the CD40 ligation of these tumor cells also induces the secretion of the CC-chemokines MDC and TARC. Supernatants from malignant cells cultured in the presence of sCD40L promote the migration of activated T cells that express CCR4, the common specific receptor for MDC and TARC. More importantly, the supernatants from CD40-stimulated tumor cells also support the transendothelial migration of autologous CCR4+ antileukemia T cells. Therefore, the results demonstrate that the delivery to leukemia cells of a single physiologic signal, that is, CD40 cross-linking, simultaneously improves tumor cell immunogenicity and induces potent chemoattraction for T cells.
Characterization of thymus-seeding precursor cells from mouse bone marrow
Authors: Mori Shinichiro , Shortman Ken , Wu Li 
Published: July 2002

AbstractThe nature of the cells that seed the thymus of an irradiated recipient after intravenous (IV) transfer of bone marrow (BM) cells was investigated using 2 approaches. First, direct entry of a small number of donor BM cells into the thymus was tracked using a Ly-5 marker. Second, secondary IV transfer of the seeded thymus cells into a secondary recipient was used as an assay for precursor activity. A range of cell types was found to enter the recipient thymus initially, including B-lineage cells and myeloid cells, but T precursors were undetectable by flow cytometry over the first few days. Although all cells initially entering the thymus proliferated, no sustained thymus reconstitution was seen until day 4, when recognizable T-lineage precursors began to appear. The secondary transfer assays revealed the presence of lymphoid precursors in the recipient thymus, including T, NKT, NK, and B precursor activity, with a notable early burst of B-lineage generative capacity. There was no evidence of sustained myeloid precursor or multipotent stem cell activity, even though these were seen if BM cells were injected directly into the recipient thymus rather than introduced into the bloodstream. It is concluded that even though many cell types may initially enter an irradiated thymus, the thymus acts as a sieve, allowing lymphoid precursors, but not multipotent stem cells, to seed the environmental niches that permit selected precursor cell development and thymus reconstitution.
Platelet-associated anti–GPIIb-IIIa autoantibodies in chronic immune thrombocytopenic purpura recognizing epitopes close to the ligand-binding site of glycoprotein (GP) IIb
Authors: Kosugi Satoru , Tomiyama Yoshiaki , Honda Shigenori , Kato Hisashi , Kiyoi Teruo , Kashiwagi Hirokazu , Kurata Yoshiyuki , Matsuzawa Yuji 
Published: July 2002

AbstractLocalization of epitopes for platelet-associated (PA) anti–GPIIb-IIIa (αIIbβ3) autoantibodies in chronic immune thrombocytopenic purpura remains elusive. Previous studies suggest that PA antibodies recognize the tertiary structure of intact glycoprotein (GP) IIb-IIIa. To localize their epitopes using antigen-capture enzyme-linked immunosorbent assay (ELISA), the reactivity of 34 PA anti–GPIIb-IIIa antibodies was examined with recombinant GPIIb-IIIa having a defect in ligand-binding sites in either GPIIb or GPIIIa, and no major conformational change was induced: KO variant GPIIb-IIIa was attributed to a 2–amino acid insertion between residues 160 and 161 in the W3 4-1 loop in GPIIb, and CAM variant GPIIb-IIIa was attributed to D119Y in GPIIIa. In one third (11 of 34) of the patients, PA antibodies showed a marked decrease (less than 50%) in reactivity with KO compared with wild-type GPIIb-IIIa. Their reactivity was also impaired against GPIIbD163A-IIIa. In sharp contrast, they reacted normally with CAM GPIIb-IIIa. OP-G2, a ligand-mimetic monoclonal antibody, markedly inhibited their binding to GPIIb-IIIa in patients with impaired binding to KO GPIIb-IIIa, but small GPIIb-IIIa antagonists did not. In addition, a newly developed sensitive ELISA indicated that autoantibodies showing impaired binding to KO are more potent inhibitors for fibrinogen binding. The present data suggest that certain PA anti–GPIIb-IIIa autoantibodies recognize epitopes close to the ligand-binding site in GPIIb, but not in GPIIIa.
Primary cutaneous lymphomas: applicability of current classification schemes (European Organization for Research and Treatment of Cancer, World Health Organization) based on clinicopathologic features observed in a large group of patients
Authors: Fink-Puches Regina , Zenahlik Paulus , Bäck Barbara , Smolle Josef , Kerl Helmut , Cerroni Lorenzo 
Published: July 2002

Abstract
Classification of primary cutaneous lymphomas (PCLs) is the subject of ongoing controversy. Based on a series of 556 patients, the applicability of the European Organization for Research and Treatment of Cancer (EORTC) classification for PCLs was assessed and compared to the proposed World Health Organization (WHO) classification of hematologic malignancies. The large majority of patients could be properly classified according to the scheme proposed by the EORTC. Comparison of estimated 5-year survival for specific diagnostic categories of PCLs demonstrated nearly complete concordance of the present results with those of the EORTC study for most of the indolent cutaneous T-cell lymphomas and cutaneous B-cell lymphomas, whereas differences were found for mycosis fungoides-associated follicular mucinosis and Sezary syndrome. A few patients with newly described entities (CD8+ epidermotropic cytotoxic T-cell lymphoma, primary cutaneous natural killer/T-cell lymphoma) could not be classified according to the EORTC scheme. Comparison of the EORTC with the WHO classification showed that the EORTC scheme allows a more precise categorization of the patients, especially for cutaneous B-cell lymphoma. In conclusion, the study confirmed that the EORTC classification allows a better management of patients with PCL. Small amendments to that classification should be carried out to account for recently described entities and to unify some of the diagnostic categories.
Creating accessible and inclusive undergraduate studentship opportunities: the ENRRICH experience
Authors: Beattie Robert , Russell Kelly , Wittmeier Kristy , Mitchell-Dueck Jenna , Cheung Kristene , Rastegar Mojgan , Slike Alana , Williams Valerie , Chan Ming-Ka , Wilson Mary , Protudjer JLP 
Published: February 2025

Abstract
Recognizing the systemic exclusion of structurally oppressed groups from academic awards, the ENRRICH (Excellence in Neurodevelopment and Rehabilitation Research In Child Health) summer studentship emphasized the inclusion of structurally oppressed groups. Herein, we outline the processes in creating this funding opportunity, and plans for improvement, including enhanced representation among supervisors.
Zero-trust based dynamic access control for cloud computing
Authors: Wang Ri , Li Chen , Zhang Kun , Tu Bibo 
Published: February 2025

Abstract
Most corporations and organizations rely heavily on access control to protect data accessibility and enable resource sharing across networks and departments. However, with the development of cloud computing, traditional boundary protection struggles to mitigate the increasing attacks and threats. In addition, most existing dynamic access control methods match static rules with dynamic metrics, which cause system damage through their delayed responses to threats and attacks. The zero-trust architecture (ZTA) provides continuous authentication and dynamic authorization for all users to accommodate the security demands of cloud computing. Drawing inspiration from the ZTA, we first present a TBAC (Trust-based Access Control) model and design a trust assessment methodology to update user trustworthiness. Then, we introduce dynamic rules in the TBAC model to implement a dynamic access control system DR-TBAC (TBAC with Dynamic Rule). We apply the DQN (Deep Q-Network) algorithm to dynamically update the trust thresholds based on static rules comparing dynamic trust with predefined trust thresholds to achieve adaptive access control policies. In this paper, we rebuild the cloud security access environment from the perspective of dynamic trust and rule optimization and strengthen the constraints on user behaviors throughout the access control lifecycle of cloud computing. Finally, a thorough analysis and assessment regarding offline training models and the online deployment of the DR-TBAC system into the cloud platform highlight its security and accuracy relative to baseline models.
Access to extension and advisory services by emerging livestock farmers in uThungulu district municipality of KwaZulu-Natal
Authors: Maake M.M.S. , Nkosi N.Z. , Antwi M.A. , Masafu M.M. , Rubhara T.T. 
Published: February 2025

Lack of access to agricultural extension and advisory services has been identified as one of the major challenges facing emerging farmers in South Africa. The purpose of this study was to determine the level of access to these services by emerging livestock farmers in uThungulu District Municipality of KwaZulu-Natal. A survey design was used with face-to-face interviews to collect data using a semi-structured questionnaire. A sample of 1 437 was randomly selected from 4 792 emerging livestock farmers in the district. Descriptive statistical analyses were performed using Statistical Package for the Social Sciences (SPSS) version 23 software. The results show that more than 90% of emerging livestock farmers in the district had high access to public extension and 14% had access to private extension, in addition. About 32% of the respondents also had access to extension and advisory services from agricultural cooperatives. There was an improvement in access to these services by emerging farmers compared to the past. The involvement of the private sector and cooperatives in rendering extension and advisory services to emerging livestock farmers shows that various stakeholders are involved in improving emerging livestock farmers in the province.
A Review of the Agroecological Farming System as a Viable Alternative Food Production Approach in South Africa
Authors: Rudolph M. , Muchesa E. 
Published: February 2025

Input-intensive production systems characterise industrial agriculture as an unsustainable means to address food and nutrition security and sustainable livelihoods. Extensive empirical evidence supports the diversification and reorientation of industrial agriculture and incorporates ecological practices, viewed as essential for achieving balanced and productive farming systems. An agroecological farming system is a viable alternative approach that can improve food production, especially for the most vulnerable communities and households. Furthermore, substantial proof and supporting evidence show that such a system is key to increasing dietary diversity at the local level and reducing the multiple health and environmental risks stemming from industrial agriculture. This paper, therefore, aims to demonstrate the benefits of the agroecology food system through an evidenced-based approach that shows how the broader agricultural network structures can play a meaningful role, particularly for impoverished households in today’s reality. The methodology is centred on a structured literature review that analyses urban agriculture, agroecology and food insecurity. Notably, ground-truthing, practical experiences and field observation of agroecological farming were deployed. This paper emphasises the practical application of the agroecological approach in urban and peri-urban settings. Several evaluation reports on local and provincial initiatives show that very few households engage in food gardens and urban agriculture. These households do not use their backyards or nearby open spaces for several reasons, such as stringent city by-laws, restricted access to land, little or no knowledge of innovative or alternative farming practices, and a general lack of interest. Furthermore, limited resources such as water and energy and lack of capacity building and training implementation are additional constraints hampering small-scale food gardens and farms in other settings. The Agroecology systems approach is viewed as one of the key solutions to tackling these problems.
The Operational Elements of the Vegetable Cooperatives: The Case of Agricultural Cooperative Societies in King Sabata Dalindyebo Local Municipality, Eastern Cape Province
Authors: Sohuma A. , Yusuf S.F.G. , Popoola O.O. 
Published: February 2025

Cooperatives are typically established to help create jobs and improve their members' economic and social conditions, among various other roles. Farmers’ cooperative societies play a vital role in enhancing the livelihood of resource-poor farmers. The government has initiated various support programmes to assist agricultural cooperative societies to remain viable; however, many cooperatives continue to flounder while some have collapsed. This study identifies operational components like members' roles, cooperative constitutions and decision-making processes, record-keeping, education and training of members, farm and financial management and level of extension service involvement as critical roles in sustaining agricultural cooperatives. Therefore, this study's objective was to assess the key operational components of vegetable cooperative societies and the level of extension support provided to the cooperatives in the study area. Ten functional vegetable cooperatives in the municipality were purposively selected for the study. At the same time, data for the survey was obtained from the board of directors and members of the cooperatives. Data was collected using semi-structured questionnaires consisting of closed and open-ended questions. The presentation of results was done using simple descriptive statistical tools. The study outcome shows that about 50% of the cooperatives noted that members were largely involved in the daily running of the cooperatives, governance, and decision-making processes. However, many cooperatives are constrained by the lack of training of its members on conflict resolutions (90%), with about 30% and 40% not receiving training on record keeping and financial management, respectively. The role of extension services towards the sustainability of the cooperatives is crucial. Most (80%) of the cooperatives indicated some level of interaction between the cooperatives and extension personnel, albeit the need to improve the frequency of extension visits, training, and follow-up appointments. The result of this study implies that cooperatives in the region need to improve in key operational areas. Extension personnel need to be more available to support cooperative activities effectively.
A retrospective analysis of vaginal birth after caesarian-predictors of success and its outcomes
Authors: Gautham Kavitha , Thiruvengadam Nalini , Vishwanathan Ramya , Jeeshiya  , Ganesh Swarna 
Published: February 2025

Background: A repeat caesarian delivery (C-section) after a previous C-section increases maternal and foetal complication and poses an enormous burden on the economy of nations and individuals. The success of vaginal birth after C-section (VBAC) is affected by various antepartum, intrapartum factors as well as the difference in the obstetric population. This study aims to analyse the maternal and neonatal outcomes and factors associated with successful VBAC.
Methods: The study is a retrospective analysis of 12 months hospital data conducted at BloomLife hospital, a private multispeciality hospital at Chennai during September 2022 to September 2023. The inclusion criteria were pregnant women with gestational age of 37±40 weeks who had intention for vaginal delivery, previous non recurrent indicating pregnancy, inter-delivery interval of at least 2 years, cephalic presentation, low risk pregnancies. High risk maternal and fetal factors were excluded from the study.
Results: Among 204 women who had previous history of LSCS, 124 women were offered a trial of labour after caesarean (TOLAC) among which 56 (45%) of them successfully delivered through VBAC. Out of 124 women, 44 women out of 124, had opted for holistic approach during antenatal and intrapartum period among which n=27 (67.5%), had a statistically significant successful VBAC (Chi-square statistic is 6.25 p=0.012, significant at p<0.05). The most common reason for repeat C-section after TOLAC were labor dysfunction and fetal distress (n=2, 3.5%). Incidence of maternal complications in the study was atonic postpartum hemorrhage (n=7, 12.5%) that was well managed medically. All the babies had an Apgar >7 at 5 minutes. There was no maternal or neonatal mortality.
Conclusions: Statistically significant rate of success was found in women who went into spontaneous labor and had antenatal and intrapartum holistic interventions. The study results demonstrates that an integrated approach with qualified birth support team will improve the success rate as well as reduce the maternal and fetal morbidity.
As Diretrizes Curriculares Nacionais para a Educação das Relações Étnico-Raciais e a Educação Infantil na produção científica brasileira
Authors: Cruz Rosimeire Costa de Andrade , Colares Maria Lília Imbiriba Sousa , Pereira Jorgiana Ricardo 
Published: February 2025

Considerando que as Diretrizes Curriculares Nacionais para a Educação das Relações Étnico-Raciais e para o Ensino de História e Cultura Afro-Brasileira e Africana - DCNERER (Resolução nº 1, 2004) destinam-se a toda educação básica e que a Educação Infantil (E.I.) figura apenas duas vezes neste documento, o artigo objetiva responder a indagação: “a quase ‘invisibilidade’ da E.I. nas DCNERER encontra equivalência nas pesquisas produzidas nos cursos de pós-graduações stricto sensu no Brasil?”. A fonte dos dados foi o Catálogo de Teses e Dissertações da Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). Trata-se de uma pesquisa do tipo “Estado da Arte”, com inspiração em Silva, Régis e Miranda (2018); Abreu e Mattos (2008); e Gomes (2012). Seus resultados confirmaram a equivalência da “quase invisibilidade” da Educação Infantil na produção acadêmica e apontaram a fragilidade na formação dos docentes e a ausência de “vontade política” como principais obstáculos à implementação da lei. Tendo em conta a quantidade de pesquisas identificadas no banco de dados consultado, as publicações que compõem o corpus desse texto evidenciam vários desafios para a academia, a legislação e as instituições de Educação Infantil e de formação de professores.
Analisis Buku Ajar Sejarah Kebudayaan Islam Menurut Badan Standar Nasional Pendidikan
Authors: Fahrudin Imam 
Published: February 2025

This study was conducted to find out how the feasibility of the ministry of religius Affairs of the republik of Indonesia with the criteria of teaching book According to BNSP. This study is considered important which given the feasibility of teaching book should refer to the criteria of BNSP. The research was conductedÂ With the qualitative approach and used literature research in data mining. The research method is conducted in three phases, they are:1) analyzing students books SKI class XIII MTS, 2) develop instrument of teaching book criteria According to BNSP, 3) relevant data collection, grouping data, provide assessment and explanation in narative. The feasibility rate of the teaching book in value with the predicate is less decent, decent enough, decent, and very decent. This study obtained the result that the conformity of Islamic cultural history book. According to BNSP has a very decent criteria to be used as teaching materials with the value of 93,02.
Peran Ustaz Menanamkan Nilai-nilai Religius di Pondok Pesantren Tarbiyatul Athfal Kuningan Jawa Barat
Authors: Fiddinillah Arrizqi , Syathori Ahmad , Jannah Darrotul 
Published: February 2025

Ustaz is the most influential person in the process and quality of educational outcomes. Therefore, the role ofustaz is needed as someone who is able toinstilreligious valuesÂ forstudents. One of the efforts of Tarbiyatul Athfal Islamic Boarding School by holding religious activities so that there is no moral decline. This study aims to determine the rolesof ustaz in instilling religious values, someÂ religiousstudents activitiesthat are able to increase the religious value of students and teacher obstacles in instilling religious values. The researchuseddescriptive qualitativemethod. Data collection techniquesÂ usedparticipatory observation, in-depth interviews, documentation. The data analysis technique utilizeddata reduction, data presentation, and conclusion drawing.Â The research showed that the role of the ustaz in instilling religious valuesÂ through religious activities in students at the Tarbiyatul Athfal Islamic Boarding School is giving love to students like their own children, setting a good example, paying attention, advising, protecting their students, not expecting remuneration, and directing the students study solely because of Allah SWT with religious valuesÂ that are instilled in students through religious activities, namely the value of worship, the spirit of jihad, morals, exemplary, sincerity.