bluuebunny/crossref_metadata_2025_split huggingface dataset:
Tsukushi is essential for the development of the inner ear
Authors: Miwa Toru , Ohta Kunimasa , Ito Naofumi , Hattori Satoko , Miyakawa Tsuyoshi , Takeo Toru , Nakagata Naomi , Song Wen-Jie , Minoda Ryosei 
Published: March 2020

AbstractTsukushi (TSK)—a small, secreted, leucine-rich-repeat proteoglycan—interacts with and regulates essential cellular signaling cascades. However, its functions in the mouse inner ear are unknown. In this study, measurement of auditory brainstem responses, fluorescence microscopy, and scanning electron microscopy revealed that TSK deficiency in mice resulted in the formation of abnormal stereocilia in the inner hair cells and hearing loss but not in the loss of these cells. TSK accumulated in nonprosensory regions during early embryonic stages and in both nonprosensory and prosensory regions in late embryonic stages. In adult mice, TSK was localized in the organ of Corti, spiral ganglion cells, and the stria vascularis. Moreover, loss of TSK caused dynamic changes in the expression of key genes that drive the differentiation of the inner hair cells in prosensory regions. Finally, our results revealed that TSK interacted with Sox2 and BMP4 to control stereocilia formation in the inner hair cells. Hence, TSK appears to be an essential component of the molecular pathways that regulate inner ear development.
Evaluation of Germline Genetic Testing Criteria in a Hospital-Based Series of Women With Breast Cancer
Authors: Yadav Siddhartha , Hu Chunling , Hart Steven N. , Boddicker Nicholas , Polley Eric C. , Na Jie , Gnanaolivu Rohan , Lee Kun Y. , Lindstrom Tricia , Armasu Sebastian , Fitz-Gibbon Patrick , Ghosh Karthik , Stan Daniela L. , Pruthi Sandhya , Neal Lonzetta , Sandhu Nicole , Rhodes Deborah J. , Klassen Christine , Peethambaram Prema P. , Haddad Tufia C. , Olson Janet E. , Hoskin Tanya L. , Goetz Matthew P. , Domchek Susan M. , Boughey Judy C. , Ruddy Kathryn J. , Couch Fergus J. 
Published: March 2020

PURPOSE To determine the sensitivity and specificity of genetic testing criteria for the detection of germline pathogenic variants in women with breast cancer. MATERIALS AND METHODS Women with breast cancer enrolled in a breast cancer registry at a tertiary cancer center between 2000 and 2016 were evaluated for germline pathogenic variants in 9 breast cancer predisposition genes ( ATM , BRCA1, BRCA2, CDH1, CHEK2, NF1, PALB2, PTEN, and TP53). The performance of the National Comprehensive Cancer Network (NCCN) hereditary cancer testing criteria was evaluated relative to testing of all women as recommended by the American Society of Breast Surgeons. RESULTS Of 3,907 women, 1,872 (47.9%) meeting NCCN criteria were more likely to carry a pathogenic variant in 9 predisposition genes compared with women not meeting criteria (9.0% v 3.5%; P < .001). Of those not meeting criteria (n = 2,035), 14 (0.7%) had pathogenic variants in BRCA1 or BRCA2. The sensitivity of NCCN criteria was 70% for 9 predisposition genes and 87% for BRCA1 and BRCA2, with a specificity of 53%. Expansion of the NCCN criteria to include all women diagnosed with breast cancer at ≤ 65 years of age achieved > 90% sensitivity for the 9 predisposition genes and > 98% sensitivity for BRCA1 and BRCA2. CONCLUSION A substantial proportion of women with breast cancer carrying germline pathogenic variants in predisposition genes do not qualify for testing by NCCN criteria. Expansion of NCCN criteria to include all women diagnosed at ≤ 65 years of age improves the sensitivity of the selection criteria without requiring testing of all women with breast cancer.
Serum Angiopoietin-like Protein 6, Risk of Type 2 Diabetes, and Response to Hyperglycemia: A Prospective Cohort Study
Authors: Fan Kang-Chih , Wu Hung-Tsung , Wei Jung-Nan , Chuang Lee-Ming , Hsu Chih-Yao , Yen I-Weng , Lin Chia-Hung , Lin Mao-Shin , Shih Shyang-Rong , Wang Shu-Huei , Chang Tien-Jyun , Li Hung-Yuan 
Published: March 2020

Abstract

Context
Angiopoietin-like protein 6 (ANGPTL6) is a hepatokine that improves insulin sensitivity in animals. However, serum ANGPTL6 concentration was found to be higher in human participants with diabetes or metabolic syndrome in cross-sectional studies, implying that ANGPTL6 may be induced to counteract hyperglycemia.

Objective
To investigate whether serum ANGPTL6 can predict incident diabetes and explore whether glucose or insulin can regulate ANGPTL6 expression and secretion.

Design
This cohort study included adults without diabetes at baseline who were followed every 2 years for incident diabetes. Serum ANGPTL6 concentrations were measured at baseline and during oral glucose tolerance tests (OGTTs). A hepatic cell line, HepG2, and diet-induced obesity mouse model were used to evaluate the response of ANGPTL6 expression and secretion to hyperglycemia and the metabolic syndrome.

Results
We recruited 1103 participants without diabetes at baseline. During the 4.22-year follow-up, 113 (10.2%) participants developed incident diabetes. Serum ANGPTL6 was negatively associated with the incidence of diabetes (adjusted hazard ratio, 0.77; P = 0.042). However, serum ANGPTL6 level was higher in participants with prediabetes (P = 0.018) and was elevated during OGTT. In HepG2 cells, treatment with glucose, but not insulin, induced ANGPTL6 expression. Hepatic ANGPTL6 expression and serum ANGPTL6 concentrations were significantly higher in mice fed with a high-fat diet than in those fed with a standard chow (both P &lt; 0.05).

Conclusion
A high serum ANGPTL6 level is associated with a low incidence of diabetes in humans. ANGPTL6 is expressed and secreted in response to hyperglycemia to maintain glucose homeostasis.
Neonatal Macrosomia is an Interfering Factor for Analytes on the Colorado State Newborn Screen
Authors: Wright Erica L , Baker Peter R 
Published: March 2020

Abstract

Purpose
Neonatal macrosomia is a known complication of maternal obesity and gestational diabetes, and it is a risk factor for obesity and diabetes in offspring. Amino acids and acylcarnitines are biomarkers for obesity in children and adults. These analytes, which are also routinely obtained on the newborn screen, have not been well-characterized in macrosomic newborns. The impact of macrosomia on rates of false-positive results in the newborn screen has also not been well-studied. We test the hypothesis that macrosomia is an interfering factor for amino acids and/or acylcarnitines on the newborn screen.

Methods
Newborn screening analytes determined by tandem mass spectroscopy were obtained from the Colorado Department of Public Health and Environment archives (2016–2018). This included metabolite concentrations obtained at 24–72 hours of life from newborns with birth weight 2500 to 3999 g (nonmacrosomic, n = 131 896) versus 4000 to 8000 g (macrosomic, n = 7806). Mother/infant phenotypic data were limited to information provided on the newborn screening dried blood spot card. Data were analyzed using Student t-test and chi-squared analysis.

Results
Macrosomic newborns had elevations in C2, C3, dicarboxylic, and long-chain acylcarnitines (specifically C16 and C18 species). C3 and C18:1 were 2 to 3 times more likely to be above predetermined state cutoffs in macrosomic versus nonmacrosomic newborns (both male and female).

Main conclusions
Macrosomia is an interfering factor for the analytes C3 and C18:1, leading to higher risk of false-positive results for methylmalonic/propionic acidemia and carnitine palmitoyl transferase type 2 deficiency, respectively. Analyte patterns found in macrosomic neonates correspond with similar analyte patterns in obese children and adults.
Correlation between ways of coping and quality of life in Iranian husbands of women with breast cancer
Authors: Shamloo Marzieh Beigom Bigdeli , Nasiri Morteza , Maneiy Mohammad , Kiarsi Maryam , Madmoli Yaghoub 
Published: March 2020

Background: Palliative nursing care provides the best possible quality of life (QoL) for patients who face life-threatening conditions, such as breast cancer, and their families. It seems that coping with breast cancer can affect couples' QoL. Hence, this study aimed to assess the potential role of ways of coping (WOC) in QoL among husbands of women with breast cancer. Method: In this cross-sectional study, 150 men whose wives were affected by non-metastatic breast cancer and were at least 4 months post-diagnosis, were recruited. The Persian version of the WOC questionnaire (WOCQ) and the World Health Organization's QoL brief questionnaire (WHOQoL-BREF) were used to measure WOC and QoL. ThePearson correlation test was applied to assess bivariate correlation of the variables. Results: A significant direct correlation was found between the total WHOQoL-BREF score and all subscales of WOCQ, except escape-avoidance coping (r=−0.017, P=0.830). Most dimensions of the WHOQoL-BREF and WOCQ subscales were correlated significantly and directly. Nevertheless, escape-avoidance coping had a significant indirect correlation with the physical dimension of WHOQoL-BREF (r=−0.220, P=0.007). Conclusions: Findings indicated a need for coping-based interventions in palliative nursing to improve QoL in husbands of women with breast cancer.
Contributing Factors of Secondary Students’ Attitude towards Mathematics Contributing Factors of Attitudes towards Mathematics
Authors: Davadas Shamila Dewi , Lay Yoon Fah 
Published: March 2020

<p style="text-align:justify">The research investigated the effect of socioeconomic status, gender, perceived parental influences, teacher affective support, classroom instruction and previous achievement on students’ attitude towards mathematics. The comparison of these effects was also done between urban and rural school students. This research employed a cross-sectional quantitative design based on a structural equation modelling approach. The sample consisted of 808 students from ten secondary schools in Sabah, three of which were urban and seven were rural schools. Findings showed positive relationships exist between perceived parental influences (r = .231), teacher affective support (r = .242), classroom instruction (r = .439), and previous achievement (r = .284) with students’ attitude towards mathematics. The multigroup analysis for urban and rural students showed similar results as the whole student group. However, for urban students, classroom instruction (r = 0.352) and previous achievement (r = -0.363) had the greatest impact on attitude towards mathematics. For rural students, the highest impact on attitude towards mathematics was from classroom instruction (r = 0.452) and teacher affective support (r = 0.246). The least impact for both groups was perceived parental influence. This study implied that factors affected students’ attitude towards mathematics in rural and urban secondary students are different</p>
Discrete Element Modeling of Seed Metering as Affected by Roller Speed and Damping Coefficient
Authors: Guzman Leno J. , Chen Ying , Landry Hubert 
Published: March 2020

Abstract. The development of highly efficient seed metering is required to meet the demands of modern seeding equipment. The discrete element method (DEM) was used to simulate metering of seeds with a fluted roller meter. This approach was chosen due to its capability to accurately represent granular material flow. The contact model selected for the DEM simulation was the linear rolling resistance model. Angle of repose experimental tests and simulations were performed to calibrate the rolling friction coefficient for peas. The calibrated value for the rolling friction coefficient was 0.016. A 192 mm cross-section of an air cart seed roller and housing was defined as the domain of the simulation. Sensitivity analysis showed that simulated mass flow rates were not sensitive to the selected damping coefficients (0.2, 0.5, and 0.8). Sensitivity indicator values varied between -0.049 and 0.088 for the range of damping coefficients and roller speeds studied. The simulated geometry of the seed meter and housing resulted in a steady flow of seeds, with discharged mass increasing linearly. The simulated mass flow rates were 34.0, 72.3, 110.4, 147.3, and 182.0 g s-1 for roller speeds of 10, 20, 30, 40, and 50 rpm, respectively. An experiment was performed to validate the simulation results. The predicted mass flow rate values of the simulation were within 10 g s-1 of the experimental results, with the largest relative error being 16.5%. Keywords: DEM, Damping, Metering, Peas, Rolling friction coefficient, Seed, Simulation.
Analysis and Design of a Spatial Planetary Noncircular Gear Train for Rice Seedling Transplanting Based on Three Given Positions
Authors: Sun Liang , Zhou Yuzhu , Huang Hengmin , Wu Chuanyu , Zhang Guofeng 
Published: March 2020

HighlightsA method for solving a spatial transplanting mechanism with noncircular gears is proposed.A new mechanism for transplanting rice pot seedlings is proposed.A trajectory with a small lateral displacement at the preparation phase is obtained.The working performance is validated by simulations and field tests.Abstract. This study proposes a method of solving the parameters of a spatial planetary gear train with noncircular gears to meet the requirements of wide-narrow row pot seedling transplanting (WPST). First, the planetary gear train was simplified to a spatial open-chain 2R mechanism (planetary carrier). A kinematic model of the 2R mechanism was derived from the three given homogeneous matrices describing the spatial position and attitude information of grasping, extracting, and planting seedlings. Second, the length of each link, attitude of each rotation axis, and relative initial angles were calculated. The model for solving the transmission ratio was deduced. A spatial planetary gear train configuration with a single planet carrier and two-stage driving was derived, in which the middle axis could be determined by combining the selected configuration, represented trajectory, and transmission ratio of each gear pair. Finally, a planetary gear train combining a noncircular gear pair and a helical gear pair was used in the WPST mechanism design. Simulations and tests conducted on a prototype confirmed the correctness of the theoretical model and the practicality of the design. Keywords: Noncircular gear, Planetary gear train, Spatial trajectory, Transmission ratio, Transplanting mechanism.
Two-lines method for estimation of plasma temperature and characterization of plasma parameters in optically thick plasma conditions
Authors: Rezaei Fatemeh 
Published: March 2020

In this paper, the characteristics of laser-induced plasmas are studied by investigation of the spectral line features in laser-induced breakdown spectroscopy (LIBS) experiments. The plasma is produced by focusing of a Nd:YAG laser on standard Al-alloy samples at 30 mJ energy. Here, with the assumption of having a homogenous plasma and by using a semiemperical technical method, the plasma temperature is calculated by the proposal of a new two-lines method. Moreover, by utilizing some theoretical equations, the plasma parameters and the self-absorption magnitude are evaluated according to the radiative transfer equations in local thermodynamic equilibrium (LTE) conditions. The main advantages of this method are that without discrimination between thin plasmas and thick ones, and as well as without straight quantification of the degree of self-absorption, the plasma temperature can be calculated. The results showed that determination of the intensities of the spectral lines, transition parameters, and Stark broadening parameter is adequate for plasma characterization in a typical LIBS experiment.
ARUS SELATAN JAWA
Authors: Surinati Dewi , Wijaya Jaka Harry Mulyanto 
Published: March 2020

SOUTH JAVA CURRENT. Indian Ocean is influenced by the monsoon cycle. Monsoon cycle that occurs in the Indian Ocean influences the current patterns. Since a very strong westerlies occurs in The Tropical Convergence Zone (TCZ) at the equator, wyrtki jet is formed in the 40° - 80° W Indian Ocean on a transitional season. Its branchs are possibly formed when the wyrtki jet is in the equatorial west coast of Sumatra to the north and south along the western coast of Sumatra which eventually creates South Java Current (SJC). SJC flows southeastward during December–April and northwestward during June–October, when it is associated with coastal upwelling. SJC develop upwelling on a seasonal basis.
PENCEMARAN PLASTIK DI LAUT
Authors: Cordova Muhammad Reza 
Published: March 2020

PLASTICS POLLUTION IN THE SEA. Indonesia is considered as the second biggest plastic waste producer in the sea. Information on waste pollution and its impact on marine organisms in Indonesia is still limited. Waste disposal and solid waste (plastics) into the sea are continuously occuring; elevating the thoughts of global impact of plastic waste contamination. Increased use of plastic is a consequence of the development of technologies, industries and also the population. This paper is expected to provide knowledge and information of plastics that exist in the sea, especially Indonesian Sea.
Treatment Of Palatoplasty Repair With Von Langenbeck Technique And Hemostatic Absorbable Gelatin: Case Report And Literature Reviews
Authors: Ibnu Ikhsan M. Idris , Subarno Saktrio D. 
Published: March 2020

Background: Cleft is a common congenital abnormality in the lip and palate. Cleft palate or palatoschisis is a congenital abnormality occured during pregnancy, resulting in cleft of the palate that does not coalesce into the nasal cavity area, so there is a relationship between the nasal cavity and mouth. Various surgical techniques for cleft palate correction can be used and each method has advantages and disadvantages.
Patient and Method: Here, we report a case of palatoschisis reconstruction using Von Langenbeck technique and hemostatic absorbable gelatin that was carried out at Aliyah 3 Hospital, Kendari.
Result: After initial diagnosis, the patient underwent surgery about two weeks later. Von Langenback palatoplasty was performed to close the palate defect. The defect then closed primarily with 4/0 optime and 6/0 corolene.
Conclusion: Optimal surgical planning, good patient orientation and surgical techniques that are carefully considered are essential for optimal results. Herein, we report a case of palatoschisis and reconstructive surgery using von Langenback technique performed to restore the cleft of palate.
Multiple Lower Eyelid Nodules In Breast Cancer Patient With History Of Dermal Fillers: A Case Report
Authors: Gunardi Alberta Jesslyn , Marsaulina Renate Parlene , Kevin Jonathan , Sakura Irena 
Published: March 2020

Background: Dermal filler injections have been associated with several complications, including migration to other areas and forming nodules due to the presence of foreign bodies or granulomatous reactions may develop, which encapsulates the filler. Fillers might also be associated with malignancy. The presence of such complications must be removed with reconstructive surgery.
Methods: We present a case of breast cancer patient with a history of dermal filler injection to the lower eyelid. The nodules in the lower eyelid was surgically removed, and the lower eyelid, particularly the muscle, was reconstructed to provide a good cosmetic result. The nodules were also pathologically examined to confirm the diagnosis. Reconstruction of the lower eyelid was conducted with care by noting the aesthetic aspects.
Results: A local muscle flap was easy to harvest and handle, and provided good results. It is also important to consider malignancy to treat the patient thoroughly. The pathology examination confirmed the diagnosis of metastasis. This case is unique because the nodules developed in the patient may be associated with metastasis of the cancer or the dermal filler injection.
Conclusion: Our reconstruction technique leads to a good cosmetic result. There are no sign of complications and recurrence at 3 months following the procedure.
The utility of measurement uncertainty in medical laboratories
Authors: Braga Federica , Panteghini Mauro 
Published: March 2020

Abstract
The definition and enforcement of reference measurement systems, based on the implementation of metrological traceability of patient results to higher-order (reference) methods and/or materials, together with a clinically acceptable level of measurement uncertainty (MU), are fundamental requirements to produce accurate and equivalent laboratory results. The MU associated with each step of the traceability chain should be governed to obtain a final combined MU on clinical samples fulfilling the requested performance specifications. MU is useful for a number of reasons: (a) for giving objective information about the quality of individual laboratory performance; (b) for serving as a management tool for the medical laboratory and in vitro diagnostics (IVD) manufacturers, forcing them to investigate and eventually fix the identified problems; (c) for helping those manufacturers that produce superior products and measuring systems to demonstrate the superiority of those products; (d) for identifying analytes that need analytical improvement for their clinical use and ask IVD manufacturers to work for improving the quality of assay performance and (e) for abandoning assays with demonstrated insufficient quality. Accordingly, the MU should not be considered a parameter to be calculated by medical laboratories just to fulfill accreditation standards, but it must become a key quality indicator to describe both the performance of an IVD measuring system and the laboratory itself.
General properties of autoimmune thyroid diseases and associated morbidities
Authors: Ibili Aysel Burcu Palandokenlier , Selver Eklioglu Beray , Atabek Mehmet Emre 
Published: March 2020

AbstractBackgroundAutoimmune thyroid diseases (ATDs) can be classified into two basic diseases: Graves’ disease (GD) and Hashimoto’s thyroiditis (HT). Here, we review the effectiveness of laboratory and imaging methods used for the early diagnosis of ATD and draw attention to methods that may improve screening.MethodsRetrospective data of 142 patients diagnosed with ATD between January 2010 and December 2015 at our paediatric endocrinology clinic were used. Sociodemographic characteristics, clinical findings, treatments and follow-up data of patients were statistically evaluated.ResultsOf the ATD cases, 81% (n = 115) were female. The median age was 12.5 ± 3.5 (range 1–17) years and 91% (n = 129) of patients were in puberty. There was a significant positive correlation between the height (standard deviation score) and follow-up time for patients with HT (r = 0.156, p < 0.01). Thyroglobulin antibody (TgAb) positivity was found in 75% (45/60) of females with a positive maternal ATD history (p = 0.045). Thyroid-stimulating hormone (TSH), free triiodothyronine (fT3), free thyroxine (fT4) values were significantly altered during the treatment follow-up period in female patients with GD and HT, while only fT4 values were found to be significantly altered in boys with HT.ConclusionAlthough GD and HT have similar mechanisms, they differ in terms of treatment duration and remission and relapse frequencies. Ultrasonography (USG) screening is a non-invasive procedure that is suitable for all patients with ATD. Based on our results, TgAb could be useful in the screening of girls with a history of maternal ATD.