bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of COVID-19 RT-qPCR Test in Multi sample Pools
Authors: Yelin Idan , Aharony Noga , Tamar Einat Shaer , Argoetti Amir , Messer Esther , Berenbaum Dina , Shafran Einat , Kuzli Areen , Gandali Nagham , Shkedi Omer , Hashimshony Tamar , Mandel-Gutfreund Yael , Halberthal Michael , Geffen Yuval , Szwarcwort-Cohen Moran , Kishony Roy 
Published: April 2020

AbstractBackgroundThe recent emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) led to a current pandemic of unprecedented scale. Although diagnostic tests are fundamental to the ability to detect and respond, overwhelmed healthcare systems are already experiencing shortages of reagents associated with this test, calling for a lean immediately applicable protocol.MethodsRNA extracts of positive samples were tested for the presence of SARS-CoV-2 using reverse transcription quantitative polymerase chain reaction, alone or in pools of different sizes (2-, 4-, 8-, 16-, 32-, and 64-sample pools) with negative samples. Transport media of additional 3 positive samples were also tested when mixed with transport media of negative samples in pools of 8.ResultsA single positive sample can be detected in pools of up to 32 samples, using the standard kits and protocols, with an estimated false negative rate of 10%. Detection of positive samples diluted in even up to 64 samples may also be attainable, although this may require additional amplification cycles. Single positive samples can be detected when pooling either after or prior to RNA extraction.ConclusionsAs it uses the standard protocols, reagents, and equipment, this pooling method can be applied immediately in current clinical testing laboratories. We hope that such implementation of a pool test for coronavirus disease 2019 would allow expanding current screening capacities, thereby enabling the expansion of detection in the community, as well as in close organic groups, such as hospital departments, army units, or factory shifts.
Software Testing Automation of VR-Based Systems With Haptic Interfaces
Authors: Corrêa Cléber G , Delamaro Márcio E , Chaim Marcos L , Nunes Fátima L S 
Published: April 2020

Abstract
As software systems have increased in complexity, manual testing has become harder or even infeasible. In addition, each test phase and application domain may have its idiosyncrasies in relation to testing automation. Techniques and tools to automate test oracles in domains such as graphical user interfaces are available; nevertheless, they are scarce in the virtual reality (VR) realm. We present an approach to automate software testing in VR-based systems with haptic interfaces—interfaces that allow bidirectional communication during human–computer interaction, capturing movements and providing touch feedback. It deals with the complexity and characteristics of haptic interfaces to apply the record and playback technique. Our approach also provides inference rules to identify possible faulty modules of the system under testing. A case study was performed with three systems: a system with primitive virtual objects, a dental anesthesia simulator and a game. Faulty versions of the systems were created by seeding faults manually and by using mutation operators. The results showed that 100% of the manually seeded faults and 93% of mutants were detected. Moreover, the inference rules helped identify the faulty modules of the systems, suggesting that the approach improves the test activity in VR-based systems with haptic interfaces.
Corporate Bankruptcy Prediction: An Approach Towards Better Corporate World
Authors: Alam Talha Mahboob , Shaukat Kamran , Mushtaq Mubbashar , Ali Yasir , Khushi Matloob , Luo Suhuai , Wahab Abdul 
Published: April 2020

AbstractThe area of corporate bankruptcy prediction attains high economic importance, as it affects many stakeholders. The prediction of corporate bankruptcy has been extensively studied in economics, accounting and decision sciences over the past two decades. The corporate bankruptcy prediction has been a matter of talk among academic literature and professional researchers throughout the world. Different traditional approaches were suggested based on hypothesis testing and statistical modeling. Therefore, the primary purpose of the research is to come up with a model that can estimate the probability of corporate bankruptcy by evaluating its occurrence of failure using different machine learning models. As the dataset was not well prepared and contains missing values, various data mining and data pre-processing techniques were utilized for data preparation. Within this research, the task of resolving the issues induced by the imbalance between the two classes is approached by applying different data balancing techniques. We address the problem of imbalanced data with the random undersampling and Synthetic Minority Over Sampling Technique (SMOTE). We used five machine learning models (support vector machine, J48 decision tree, Logistic model tree, random forest and decision forest) to predict corporate bankruptcy earlier to the occurrence. We use data from 2009 to 2013 on Poland manufacturing corporates and selected the 64 financial indicators to be broken down. The main finding of the study is a significant improvement in predictive accuracy using machine learning techniques. We also include other economic indicators ratios, along with Altman’s Z-score variables related to profitability, liquidity, leverage and solvency (short/long term) to propose an efficient model. Machine learning models give better results while balancing the data through SMOTE as compared to random undersampling. The machine learning technique related to decision forest led to 99% accuracy, whereas support vector machine (SVM), J48 decision tree, Logistic Model Tree (LMT) and Random Forest (RF) led to 92%, 92.3%, 93.8% and 98.7% accuracy, respectively, with all predictive financial indicators. We find that the decision forest outperforms the other techniques and previous techniques discussed in the literature. The proposed method is also deployed on the web to assist regulators, investors, creditors and scholars to predict corporate bankruptcy.
MIPPIE: the mouse integrated protein–protein interaction reference
Authors: Alanis-Lobato Gregorio , Möllmann Jannik S , Schaefer Martin H , Andrade-Navarro Miguel A 
Published: April 2020

AbstractCells operate and react to environmental signals thanks to a complex network of protein–protein interactions (PPIs), the malfunction of which can severely disrupt cellular homeostasis. As a result, mapping and analyzing protein networks are key to advancing our understanding of biological processes and diseases. An invaluable part of these endeavors has been the house mouse (Mus musculus), the mammalian model organism par excellence, which has provided insights into human biology and disorders. The importance of investigating PPI networks in the context of mouse prompted us to develop the Mouse Integrated Protein–Protein Interaction rEference (MIPPIE). MIPPIE inherits a robust infrastructure from HIPPIE, its sister database of human PPIs, allowing for the assembly of reliable networks supported by different evidence sources and high-quality experimental techniques. MIPPIE networks can be further refined with tissue, directionality and effect information through a user-friendly web interface. Moreover, all MIPPIE data and meta-data can be accessed via a REST web service or downloaded as text files, thus facilitating the integration of mouse PPIs into follow-up bioinformatics pipelines.
Comparative Trap Catches of Male Bactrocera, Dacus, and Zeugodacus Fruit Flies (Diptera: Tephritidae) With Four Floral Phenylbutanoid Lures (Anisyl Acetone, Cue-Lure, Raspberry Ketone, and Zingerone) in Queensland, Australia
Authors: Royer Jane E , Tan Keng Hong , Mayer David G 
Published: April 2020

AbstractThe male fruit fly attractants, cue-lure (CL) and raspberry ketone (RK), are important in pest management. These volatile phenylbutanoids occur in daciniphilous Bulbophyllum Thouar (Orchidaceae: Asparagales) orchids, along with zingerone (ZN) and anisyl acetone (AA). While these four compounds attract a similar range of species, their relative attractiveness to multiple species is unknown. We field tested these compounds in two fruit fly speciose locations in north Queensland, Australia (Lockhart and Cairns) for 8 wk. Of 16 species trapped in significant numbers, 14 were trapped with CL and RK, all in significantly greater numbers with CL traps than RK traps (at least in higher population locations). This included the pest species Bactrocera tryoni (Froggatt) (Diptera: Tephritidae) (CL catches ca. 5× &gt; RK), Bactrocera neohumeralis (Hardy) (Diptera: Tephritidae) and Bactrocera bryoniae (Tryon) (Diptera: Tephritidae) (CL catches ca. 3× &gt; RK), and Bactrocera frauenfeldi (Schiner) (Diptera: Tephritidae) (in Cairns—CL catches ca. 1.6× &gt; RK). Seven species were trapped with AA, and all were also caught in CL and RK traps in significantly greater numbers, with the exception of B. frauenfeldi. For this species, catches were not statistically different with CL, RK, and AA in Lockhart, and RK and AA in Cairns. Seven species were trapped with ZN, two at this lure only, and the remainder also with CL or RK but in significantly greater numbers. This is the first quantitative comparison of the relative attractiveness of CL, RK, AA, and ZN against multiple species, and supports the long-held but untested assumption that CL is broadly more attractive lure than RK.
Bridge shapes of the violin and other bowed instruments, 1400–1900: the origin and evolution of their design
Authors: Raymaekers Wim 
Published: April 2020

Abstract
As one goes back in time, the number of extant bowed instruments decreases gradually, and this is particularly true for loose parts such as bridges, pegs and tailpieces. The current article traces the development of bridges of instruments of the violin family, based on a study of extant instruments and around 800 iconographic sources. This study reveals the origins of the various elements of bridges found on modern instruments of the violin family, and reconstructs the different stages of their evolution. Many elements of the modern violin bridge were already present, sometimes hidden, in bowed-instrument bridges of the 16th century. The extensive appendices and many line drawings offer today’s researchers, makers and restorers the possibility of providing historically informed bridges that are less fantasized than those hitherto often encountered on restorations or reconstructions of old instruments.
Peak expiratory flow in the standing and sitting positions is equivalent in adults: a cross-over study
Authors: Teng Cheong Lieng , Chia Kun Mun , D’Cruz Jasmine , Gomez Colin Adrian , Muthusamy Nesalatchumy , Saadon Nur Syahida , Jali Noraini Mat , Loh Li Cher 
Published: April 2020

AbstractBackgroundIt is uncertain whether peak flow measurement is best done in the standing or sitting position.MethodsIn this cross-over study, study participants were randomized to perform the initial peak expiratory flow (PEF) measurement in either standing or sitting position. The highest of three readings in each position were compared using paired t-test. A mean difference of &lt;±25 l/min was set as the equivalence limits. Test of equivalence of standing and sitting PEF measurements was done using MedCalc Software. Test of agreement of standing and sitting PEF was assessed by Lin’s concordance correlation coefficient and Bland–Altman limits of agreement.ResultsOf the 100 study participants, 50% of them had asthma. There was a statistically significant difference between the standing and sitting PEF in adults suffering from asthma [mean difference 11 l/min, 95% confidence interval (CI) = 4 to 19], but not in the healthy individuals (mean difference 3 l/min, 95% CI = −6 to 12). The observed differences in PEF were small and may not be clinically important. In adults with and without asthma, the standing and sitting PEF were highly correlated and satisfied the test of equivalence.ConclusionsThe PEF in the standing and sitting positions was equivalent in adults. Therefore, performing PEF in either position is acceptable. However, health care practitioners should be aware of the small reduction in PEF when it is done in the sitting position. It is desirable that the position used is documented and the same position is used wherever possible.
Patient–Provider Discussions About Urinary Incontinence Among Older Women
Authors: Lane Giulia I , Hagan Kaitlin , Erekson Elisabeth , Minassian Vatche A , Grodstein Francine , Bynum Julie 
Published: April 2020

AbstractBackgroundUrinary incontinence (UI) is prevalent in women and has been associated with decreased quality of life and institutionalization. Despite this, and the fact that several treatment options exist, few women discuss UI with clinicians. The aim of this study was to examine the proportion of middle aged and older women with urinary incontinence who have discussed UI with clinicians, focusing on female health professionals as a way to examine this question outside of issues of health care access.MethodsData are from the Nurses Health Studies (NHS), two ongoing observational, prospective, cohort studies. The surveys collected detailed information about UI, including frequency, amount and type. Women were also asked if they had discussed UI with a clinician. We used multivariable-adjusted logistic regression to estimate odds ratios (OR) of participants reporting discussion about UI.Results94,692 women with UI aged 49–91 years old were included in this study. Of these, 34% reported that they had discussed their incontinence with a clinician. Women with daily UI had 4.4 times greater odds of discussing it with clinicians when compared to those with monthly UI (OR = 4.36, 95% confidence interval [CI] 4.06–4.69). When controlling for severity of symptoms, the oldest women, greater than eighty years, were 20% less likely to have discussed UI with their clinician, compared to the youngest women (OR = 0.81, 95% CI 0.73–0.89).ConclusionsA minority of women with UI, even among health professionals, discuss their symptoms with clinicians. Oldest women were the least likely to discuss their UI with a provider.
‘Enforcement of Patent Law in Civil Proceedings’ – A Conference Report on the Fifth Bi-national Seminar of TU Dresden and Charles University in Prague, 26 November 2019
Authors: Petrlík David , Linke David 
Published: April 2020

Abstract
What is the law good for if it cannot be enforced? This question is currently virulent in patent law, especially when it comes to the enforcement of injunctive relief claims. From the German perspective, a question arises: does a patent infringement have to automatically result in an injunction (as is the situation currently according to Sec. 139 of the Patent Law Act) or should exceptions be made in special cases? In particular, the automotive industry and also the IT sector demand a modernisation of this provision by introducing a proportionality test, as even the smallest patentable components in networked products can lead to the entire production being blocked in the event of a successful injunction action. Similar problems exist in US patent law. The enforceability of Czech patent law also faces challenges that need to be solved. For this reason, the fifth seminar on the topic ‘Enforcement of Patent Law in Civil Proceedings’ took place on 26 November 2019 at Charles University Prague.
Risk of hepatocellular carcinoma in individuals without traditional risk factors: development and validation of a novel risk score
Authors: Sinn Dong Hyun , Kang Danbee , Cho Soo Jin , Paik Seung Woon , Guallar Eliseo , Cho Juhee , Gwak Geum-Youn 
Published: April 2020

Abstract

Background
Although hepatocellular carcinoma (HCC) occurs mostly in patients with chronic hepatitis B virus (HBV) or hepatitis C virus (HCV) infection or heavy alcohol use or cirrhosis, some patients develop HCC without these risk factors. Our objective in this study was to develop and validate a new HCC risk score that could stratify HCC risk in patients who develop HCC without known risk factors.

Methods
A new HCC risk score was developed using a nationwide, population-based cohort among individuals without chronic HBV infection, chronic HCV infection, heavy alcohol use or cirrhosis (n = 467 206, derivation cohort). The performance of the HCC risk score was validated using an independent Samsung Medical Center Health Promotion Center cohort (n = 91 357, validation cohort).

Results
Multivariable Cox regression analysis identified six independent risk factors: age, sex, smoking, diabetes, total cholesterol level and serum alanine aminotransferase level. A 19-point scale for HCC risk score was developed, with 10-year risk of HCC ranging from 0.0% to 6.16% for the lowest and highest risk scores, respectively. The area under the receiver operating characteristics curve values (AUROCs) to predict HCC development were 0.83 [95% confidence interval (CI): 0.77, 0.88)] and 0.92 (95% CI: 0.89, 0.95) at 10 years in the derivation and validation cohorts, respectively. Predicted risk was well correlated with the Kaplan-Meier observed HCC risk.

Conclusions
A simple-to-use, novel HCC risk score was developed for predicting HCC development in individuals without alleged risk factors. It can be used to assess the risk of HCC in this population so that decisions about their clinical management, including risk reduction interventions, can be subsequently made.
Local Immunosuppression for Vascularized Composite Allografts: Application of Topical FK506-TyroSpheres in a Nonhuman Primate Model
Authors: Gama Amon-Ra , Ng Zhi Yang , Shanmugarajah Kumaran , Mastroianni Melissa , Randolph Mark A , Lellouch Alexandre G , Kohn Joachim , Cetrulo Curtis L 
Published: April 2020

AbstractTransplantation of vascularized composite allografts (VCAs) provides a means of restoring complex anatomical and functional units following burns and other disfigurement otherwise not amenable to conventional autologous reconstructive surgery. While short- to intermediate-term VCA survival is largely dependent on patient compliance with medication, the myriad of side effects resulting from lifelong systemic immunosuppression continue to pose a significant challenge. Topical immunosuppression is therefore a logical and attractive alternative for VCA. Current formulations are limited though, by poor skin penetration but this may be mitigated by conjugation of immunosuppressive drugs to TyroSpheres for enhanced delivery. Therefore, we investigated the topical application of FK506-TyroSpheres (in the form of a gel dressing) in a clinically relevant nonhuman primate VCA model to determine if allograft survival could be prolonged at reduced levels of maintenance systemic immunosuppression. Six Major Histocompatibility Complex (MHC)-mismatched cynomolgus macaques (Macaca fascicularis) served as reciprocal donors and recipients of radial forearm fasciocutaneous flaps. Standard Bacitracin ointment and FK506-TyroSpheres were applied every other day to the VCAs of animals in groups 1 (controls, n = 2) and 2 (experimental, n = 4), respectively, before gradual taper of systemic FK506. Clinical features of VCA rejection still developed when systemic FK506 fell below 10 ng/ml despite application of FK506-TyroSpheres and prolonged VCA survival was not achieved. However, unwanted systemic FK506 absorption was avoided with TyroSphere technology. Further refinement to optimize local drug delivery profiles to achieve and maintain therapeutic delivery of FK506 with TyroSpheres is underway, leveraging significant experience in controlled drug delivery to mitigate acute rejection of VCAs.
Perioperative Multimodal Analgesia Reduces Opioid Use Following Skin Grafting in Nonintubated Burn Patients
Authors: Lennertz Richard , Zimmerman Haley , McCormick Timothy , Hetzel Scott , Faucher Lee , Gibson Angela 
Published: April 2020

AbstractHundreds of patients are treated for burn injuries each year at University of Wisconsin School of Medicine and Public Health. Pain management is particularly challenging during dressing changes and following skin grafting procedures. We performed a retrospective chart review from January 2011 through June 2018 to evaluate the effect of nonopioid analgesic medications on opioid use in nonintubated patients. Our primary outcome was the change in opioid use following the procedure. We found that most patients (69%) report severe pain (Numeric Rating Scale ≥7) immediately after autologous skin grafting. On average, patients required an additional 52 mg of oral morphine equivalents (ME) in the 24 h after the procedure compared with the 24 h before. The use of perioperative nonopioid analgesia varied between patients (acetaminophen 29%, gabapentin 29%, ketamine 35%, and all three 8%). Patients who received either gabapentin or a combination of acetaminophen, gabapentin, and ketamine had a smaller increase in their opioid use than patients who did not receive the medications (−25 ME, 95% confidence interval [−46, −4]; P = .018 and −47 ME, [−81, −11]; P = .010, respectively). These results support using a combination of acetaminophen, gabapentin, and ketamine for perioperative analgesia in burn patients undergoing autologous skin grafting.
A Retrospective Multicenter Study of 1898 Liquefied Petroleum Gas-Related Burn Patients in Eastern China From 2011 to 2015
Authors: Jin Ronghua , Shao Jiaming , Ho Jon Kee , Yu Meirong , Han Chunmao 
Published: April 2020

Abstract
Liquefied petroleum gas (LPG) is a widely used environment-friendly fuel. Previous studies have shown an increasing number of LPG-related burns. Our study was designed to evaluate the epidemiologic pattern of these injuries and provide recommendations for burn prevention. This retrospective study included all patients with LPG-related burns from eight burn centers in Zhejiang Province, China between 2011 and 2015. Database variables included patient demographics, accident characteristics, and injury characteristics. The association between different categorical variables was identified using the chi-square test. And the association between two or more means of quantitative variables was analyzed by the one-way analysis of variance or t-test. A total of 1898 patients were included, 47.31% were males and 52.69% were females. The predominant age group was 31 to 70 years (74.50%), and the majority were poorly educated and the incidence peaked from June to September. The most common place of occurrence was home (74.08%) and gas leak (96.52%) was the most common cause. The four limbs (43.33%) were the most frequently affected areas; the mean burn area was 25.19 ± 20.97% of the total body surface area and most patients (46.89%) suffered from moderate burns. The mean length of hospital stay was 17.66 ± 16.55 days and the majority of patients (89.36%) recovered with a 0.84% mortality rate. Our findings reflected that the increase in incidence rate was alarming, and the causes resulting in LPG-related burns have not gained much attention yet. Therefore, this calls for simple but strict measures aiming at each hazardous step during the use of LPG to prevent these burn injuries.