bluuebunny/crossref_metadata_2025_split huggingface dataset:
Incidence and Risk Factors of Illnesses Presumably Caused by A SARS-CoV-2 Infection in The General Population During The Lockdown Period: A Multi-Cohort Study.
Authors: Carrat Fabrice , Touvier Mathilde , Severi Gianluca , Meyer Laurence , Jusot Florence , Lapidus Nathanael , Rahib Delphine , Lydié Nathalie , Charles Marie-Aline , Ancel Pierre-Yves , Rouquette Alexandra , de Lamballerie Xavier , Zins Marie , Bajos Nathalie 
Published: October 2020

Abstract
Background Our main objectives were to estimate the incidence of illnesses presumably caused by SARS-CoV-2 infection during the lockdown period and to identify the associated risk factors.Methods Participants from 3 adult cohorts in the general population in France were invited to participate in a survey on COVID-19. The main outcome was possible COVID-19, defined as a sudden onset of cough, fever, dyspnea, ageusia and/or anosmia, that lasted more than 3 days and occurred during the 17 days before the survey. We used delayed-entry Cox models to identify associated factors.Results Between April 2, 2020 and May 12, 2020, 279,478 participants were invited, 116,903 validated the questionnaire and 106,848 were included in the analysis. Three thousand thirty-five cases of possible COVID-19 were reported during 62,099 person-months of follow-up. The cumulative incidences of possible COVID-19 were 6.2% (95% Confidence Interval (95%CI): 5.7%; 6.6%) on day 15 and 8.8% (95%CI 8.3%; 9.2%) on day 45 of lockdown. The risk of possible COVID-19 was lower in older age groups and higher in French regions with a high prevalence of SARS-CoV-2 infection, in participants living in cities >100,000 inhabitants (vs rural areas), when at least one child or adolescent was living in the same household, in overweight or obese people, and in people with chronic respiratory diseases, anxiety or depression or chronic diseases other than diabetes, cancer, hypertension or cardiovascular diseases. Conclusion The incidence of possible COVID-19 in the general population remained high during the first two weeks of lockdown, and decreased significantly thereafter. Modifiable and non-modifiable risk factors were identified.
Implementation and Sustainability Factors of Two Early-Stage Breast Cancer Conversation Aids in Diverse Practices
Authors: Schubbe Danielle , Yen Renata W. , Saunders Catherine H. , Elwyn Glyn , Forcino Rachel , O’Malley A. James , Politi Mary C. , Margenthaler Julie , Volk Robert J. , Sepucha Karen , Ozanne Elissa , Percac-Lima Sanja , Bradley Ann , Goodwin Courtney , Muijsenbergh Maria van den , Aarts Johanna WM , Scalia Peter , Durand Marie-Anne 
Published: October 2020

Abstract
BackgroundConversation aids can facilitate shared decision making and improve patient-centered outcomes. However, there are few examples of sustained use of conversation aids in routine care due to numerous barriers at clinical and organizational levels. We explored strategies that will promote the sustained use of two early-stage breast cancer conversation aids. We examined any differences in experiences and opinions between the two conversation aids and across socioeconomic strata. MethodsWe nested this study within a randomized controlled trial evaluating two early-stage breast cancer surgery treatment conversation aids, one text-based and one picture-based, across four health systems with socioeconomically diverse patient populations. We conducted semi-structured interviews with a sample of patient participants, purposively sampled across conversation aid assignment and socioeconomic status (SES), and collected observations and field-notes. We also interviewed trial surgeons and other stakeholders. We conducted a framework analysis of all interviews, notes, and observations with two independent coders using the NOrmalization MeAsure Development through Normalization Process Theory. We also conducted an inductive analysis. We conducted additional sub-analyses based on which conversation aid was used/received and patient SES. ResultsWe conducted 73 semi-structured interviews with 43 patients, 16 surgeons, and 14 stakeholders like nurses, cancer center directors, and electronic health record (EHR) experts. Patients and surgeons felt the conversation aids should be used in breast cancer care in the future and were open to various methods of giving and receiving the conversation aid (EHR, email, patient portal, before consultation). Patients of higher SES were more likely to note the conversation aids influenced their treatment discussion, while patients of lower SES noted more influence on their decision making. Intervention surgeons reported using the conversation aids did not lengthen their typical consultation time. Most intervention surgeons felt using the conversation aids was similar to their usual care after using it a few times, and most patients felt it appeared part of their normal routine. ConclusionsKey factors that will help guide the future sustained implementation of the conversation aids include adapting to existing clinical workflows, flexibility of use, patient characteristics and communication preferences.Trial registrationNCT03136367 at ClinicalTrials.gov
Thermolabile Drug Storage in an Ambulatory Setting
Authors: Pazo-Oubiña Fernando do , Alorda-Ladaria Bartomeu , Lobon Ana Gómez , Vallespir Bàrbara Boyeras , Estelrich Maria Margalida Santandreu , Puigserver Clara Martorell , Zamora María Gómez , Bosch Pere Ventayol , Sánchez Olga Delgado 
Published: October 2020

Abstract
BackgroundMore and thermolabile drugs are becoming available, and in most cases, these medications are dispensed to ambulatory patients. However, there is no regulation once medications are dispensed to patients and little is known with regard to what happens during transport and home storage. Previous studies suggest that these drugs are improperly stored. The present study was designed to determine the storage conditions of thermolabile drugs once they are dispensed to the patient in the Hospital Pharmacy Department.MethodsThis is a prospective observational study to assess the temperature profile of 7 thermolabile drugs once they are dispensed to ambulatory patients at a tertiary care hospital. A data logger was added to the medication packaging. Temperature was considered inappropriate if one of the following circumstances were met: any temperature record less than or equal to 0ºC or over 25ºC; temperatures between 0-2ºC or 8ºC-25ºC for a continuous period over 30 minutes. The time series of temperature measurements obtained from each data logger were analyzed as statistically independent variables. The data shown did not undergo any statistical treatment and must be considered directly related to thermal measurements.ResultsOne hundred and fourteen patients were included and 107 patients were available for the analysis. On the whole, a mean of 50.6 days (SD 18.3) were measured and the mean temperature was 6.88ºC (SD 2.93). Three data loggers (2.8%) maintained all the measurements between 2ºC and 8ºC with less than 3 continuous data (< 30 minutes) out of this range but no data over 25ºC or below or equal to 0ºC. 28 (26.2%) data loggers had at least one measurement below zero, 1 data logger had a measurement greater than 25ºC and 75 (70.1%) were between 0ºC and 2ºC and/or between 8º and 25ºC for more than 30 minutes.ConclusionsOnce dispensed to patients, most thermolabile drugs are improperly stored. Future studies should focus on clinical consequences and possible solutions.
Co-culture of Vel1-overexpressed Trichoderma asperellum and Bacillus amyloliquefaciens: An eco-friendly strategy to hydrolyze the lignocellulose biomass in soil to enrich the soil fertility, plant growth and disease resistance
Authors: Karuppiah Valliappan , Zhixiang Lu , Liu Hongyi , Vallikkannu Murugappan , Chen Jie 
Published: October 2020

Abstract
Background: Retention of agricultural bio-mass residues without proper treatment could affect the subsequent plant growth. In the present investigation, the co-cultivation of genetically engineered T. asperellum and B. amyloliquefaciens has been employed for multiple benefits including the enrichment of lignocellulose biodegradation, plant growth, defense potential and disease resistance. Results: The Vel1 gene predominantly regulates the secondary metabolites, sexual and asexual development as well as cellulases and polysaccharide hydrolases productions. Overexpression mutant of the Trichoderma asperellum Vel1 locus (TA OE-Vel1) enhanced the activity of FPAase, CMCase, PNPCase, PNPGase, xylanase I, and xylanase II through the regulation of transcription regulating factors and the activation of cellulase and xylanase encoding genes. Further, these genes were induced upon co-cultivation with Bacillus amyloliquefaciens (BA). The co-culture of TA OE-Vel1 + BA produced the best composition of enzymes and the highest biomass hydrolysis yield of 89.56 ± 0.61%. The co-culture of TA OE-Vel1 + BA increased the corn stover degradation by the secretion of cellulolytic enzymes and maintained the C/N ratio of the corn stover amended soil. Moreover, the TA OE-Vel1 + BA increased the maize plant growth, expression of defense gene and disease resistance against Fusarium verticillioides and Cohilohorus herostrophus. Conclusion: The co-cultivation of genetically engineered T. asperellum and B. amyloliquefaciens could be utilized as a profound and meaningful technique for the retention of agro residues and subsequent plant growth.
Satellite View of Vegetation Dynamics and Drivers Over Southwestern China
Authors: Yan Wei , Wang Hesong , Jiang Chao , Jin Shaofei , Ai Jinlong , Sun Osbert Jianxin 
Published: October 2020

Abstract
Background: Vegetation is a key component of terrestrial ecosystems. The dynamics of vegetation can be used as an indicator of vegetation health and ecosystem stability. However, the dynamics and responses of vegetation in Southwestern China remain unclear.Methods: Both monotonic and turning point approaches were used in trend analysis to understand the dynamics of vegetation. We applied partial correlation analysis and the relative importance of climatic factors to determine the drivers of vegetation dynamics. Residual trend analysis was applied to distinguish other drivers.Results: The annual mean NDVI generally increased at a rate of 0.0011 year−1 from 1982 to 2015, but much of the study area showed the opposite trend before and after the turning point during this period. The vegetation in the western part of the study area was degraded and increased significantly in the eastern part of the study area. An increasing trend that continued to an increasing trend (II) and a decreasing trend that changed into an increasing trend (DI) were two of the largest patterns of vegetation trends, and they were mainly distributed in the eastern part of the study area. An increasing trend that changed into a decreasing trend (ID) was mainly distributed in the western part of the study area, where the vegetation types were mainly evergreen needle-leaved and grassland. Temperature was the dominant climatic factor and had a positive correlation with vegetation in the eastern part of the study area. Temperature also had some negative correlations with vegetation in western mountainous areas where surface soils are prone to droughts. Solar radiation had a positive correlation with vegetation in the western part of the study area. In addition, the regions with more intensity human activities than other areas were concentrated in the eastern part of the study area.Conclusions: The spatial distribution and trend showed high heterogeneity over Southwestern China. Temperature was the dominant climatic factor. In the eastern part of the study area, human activities played an important role in vegetation greening.
Disparity in Physician-Patient Communication by Ethnicity: Evidence from Bangladesh
Authors: Zakaria Muhammad , Karim Rezaul , Rahman Murshida , Cheng Feng , Xu Junfang 
Published: October 2020

Abstract
Background: Physician-patient communication is the primary process by which medical decision-making occurs and health outcome depends. Physician-patient communication differences may partly from the ethnic disparities. To examine this problem, this study aims to explore whether physician-patient communication differs by ethnic in primary care medical consultations. Methods: The study was conducted among the Bengali and ethnic minority patients (N = 850) who visited the physician for medical consultations. Data were collected using a structured post-consultation questionnaire. T-test was conducted to compare the communication between the Bengali and ethnic minority patients. Multiple linear regression analyses were performed to identify the factors associated with favorable communication behavior from the physicians. Results: Bengali patients received more supportive communication behaviors from the Bengali doctors than that of ethnic minority patients including physicians’ cheerful greetings, encouraging patients to express health problems and asking questions, listening carefully, responding to the questions and concerns, explaining the patients about a medical test, medication, and probable side effects, discussing the treatment options, involved the patients in decisions and spending adequate time. Results of linear regression explored that respondents’ education year, internet use, knowledge on the health problem, having a pre-plan about the content of medical consultation, information seeking about the health problem, visiting female doctors, and quiet ambience of the doctor’s room are significantly associated with better PPCB score for the Bengali patients. In contrast, age, being the resident of an urban area, perception of affecting a minor health problem, having a pre-plan about the content of medical consultation, patients’ involvement in physicians’ decision-making about the treatment, and talking time resulted in better physician-patient communication for the ethnic minority patients. Conclusion: This study suggests for reducing the disparity in the socio-economic status of the ethnic minority people through development program and teaching healthcare providers how to use patient-centered communication skills to engage their patients is one solution to improve equity in the delivery of healthcare and make sure patients are receiving high-quality treatment, no matter their race or ethnicity.
"We're Actually More of a Likely Ally than an Unlikely Ally": Relationships Between Syringe Services Programs and Law Enforcement
Authors: Franco Carol Y. , Lee-Winn Angela , Brandspigel Sara , Alishahi Musheng , Brooks-Russell Ashley 
Published: October 2020

Abstract
Syringe Services Programs (SSPs) provide sterile needles and a range of health services (e.g., HIV and HEP-C testing, overdose prevention education, provision of naloxone) to a hard-to-reach population, including people who inject drugs (PWID), aiming to prevent the transmission of infectious diseases. We performed a qualitative needs assessment of existing SSPs in the state of Colorado in 2018-2019 to describe the SSP activities, needs, and barriers. We performed semi-structured interviews with key program staff of SSPs (n=11). All interviews were digitally recorded, transcribed, and validated. Qualitative researchers coded each transcript and maintained coding consistency through coding statistics (Kappa coefficient > 0.80) between coders. Memos were written to synthesize main themes. Nearly all the SSPs discussed their relationships with law enforcement at length. All SSPs viewed having a positive relationship with law enforcement as critical to the success of their program. Main factors that influence the quality of relationships between SSPs and law enforcement included: 1) alignment in agency culture, 2) support from law enforcement leadership, 3) police officers’ participation and compliance with the Law Enforcement Assisted Diversion (LEAD) program, which provides intensive case management for low-level drug offenders, and 4) implementation of the “Needle-Stick Prevention Law” and Drug Paraphernalia Law Exemption. All SSPs expressed a strong desire to have positive relationships with law enforcement and described how a collaborative working relationship was critical to the success of their programs. Our findings suggest effective strategies to foster relationships between SSPs and law enforcement as well as key barriers to address.
Induction a Rat Model of Premature Ovarian Insufficiency (POI) Using D Galactose Feeding During the Critical Periods of Development: an Experimental Protocol
Authors: Dovom Marzieh Roatami , Noroozzadeh Mahsa , Mosaffa Nariman , Piryaei Abbas , Zadevakili Azita , Abdollahifar Mohammad-Amin , Tehrani Fahimeh Ramezani 
Published: October 2020

Abstract
Introduction: Premature ovarian insufficiency (POI) affects about 1% of women of reproductive age, with no curative treatment. Several efforts have been made to develop an animal model of POI that mimics all manifestations of this disease; however, these models are limited due to increased mortality or high toxicity. Overall, well-developed animal models may improve our knowledge of diseases and can be suitable for identifying the underlying mechanisms and detecting the optimal treatment methods. In the present study, we aimed to present a functional rat model of POI, mimicking the clinical manifestations of POI in women. Materials and Methods: This experimental study was performed in two steps. In the first step (induction of the rat POI model), a total of 40 pregnant Wistar rats were randomly divided into four groups. Three experimental groups were fed galactose-enriched diets at days 3-15 of pregnancy (group 1); on the third day of pregnancy until the end of pregnancy (group 2); and on the third day of pregnancy until the end of the weaning period (group 3). Also, group 4 (control group) was fed a standard pellet during pregnancy and breastfeeding period. In the second step of the study, the ovarian morphology (microscopic and macroscopic), reproductive hormones, and immunological and metabolic characteristics of the female offspring were examined in all experimental groups and compared with the controls at three time points, that is, postnatal days (PNDs) 45, 105, and 180 of age.Results: In this study, we presented an optimal model of POI that highly mimics the clinical manifestations of this disease in women. Conclusion: A functional rat model of POI may be suitable for increasing our understanding of this disease and filling the knowledge gaps.
Combination Therapy With Remdesivir, Dexamethasone, and Tocilizumab in Patients With Severe Corona Virus Disease 2019 in Clinical Practice
Authors: Izumo Takehiro , Inomata Minoru , Kuse Naoyuk , Awano Nobuyasu , Tone Mari , Takada Kohei , Muto Yutaka , Fujimoto Kazushi , Ueda Akihiro , Hayashi Munehiro 
Published: October 2020

Abstract
BackgroundThe use of several promising drugs for coronavirus disease 2019 (COVID-19) has emerged. However, considering the pathophysiology of COVID-19, the effect of a single agent is limited. Hence, the current study aimed to compare the clinical outcomes between patients who received combination treatment with remdesivir, dexamethasone, and tocilizumab (RDT) and those who did not.MethodsPatients who received combination therapy with RDT at Japanese Red Cross Medical Center were included in the RDT group, and those who did not in the control group. The mortality rate and presence of severe adverse events were compared between the two groups.ResultsIn total, 46 patients (n = 29, control group and n = 17, RDT group) with severe COVID-19 were enrolled in this study. The 28-day mortality rate was significantly lower in the RDT group than in the control group, with 1 (6%) and 9 (31%) deaths recorded, respectively (P = 0.04). Further, both groups did not present with severe adverse events.ConclusionsInformation on the outcomes of combination therapy with RDT was considered useful for the treatment of severe COVID-19.
Roles of ROS&nbsp;and Cell Cycle Arrest in the Genotoxicity Induced by Gold Nanorod Core/silver Shell Nanostructure&nbsp;
Authors: Wang Dan , Dan Mo , Ji Yinglu , Wu Xiaochun , Wang Xue , Wen Hairuo 
Published: October 2020

Abstract
To understand the genotoxicity induced in the liver by silver nanoparticles (AgNPs) and silver ions, an engineered gold nanorod core/silver shell nanostructure (Au@Ag NR) and humanized hepatocyte HepaRG cells were used in this study. The involvement of oxidative stress and cell cycle arrest in the DNA and chromosome damage induced by 0.4 - 20 µg.mL-1 Au@Ag NR were investigated by comet assay, γ-H2AX assay, and micronucleus test. Further, the distribution of Au@Ag NR was analyzed. Our results demonstrated that both Ag+ and Au@Ag NR led to DNA cleavage and chromosome damage (clastogenicity) in HepaRG cells, and that the Au@Ag NR retained in the nucleus may further release Ag+, aggravating the damages, which are mainly caused by cell cycle arrest and ROS formation. The results reveal the correlation between the intracellular accumulation, Ag+ ion release as well as the potential genotoxicity of AgNPs.
Interpretation Method of GATEM&nbsp;Data Based on PID Controller Iteration Downward Continuation Method&nbsp;&nbsp;
Authors: Guan Shanshan , Zhu Yu , Du Bingxuan , Li Dongsheng , Wang Yuan , WU QIONG , Ji Yanju 
Published: October 2020

Abstract
The Ground-source Airborne Time-domain Electromagnetic (GATEM) system has advantages for high efficiency and complex areas such as mountainous zone. The widely used section interpretation method, ignoring the impact of flight height, which seriously affects the interpretation and imaging accuracy of shallow anomalies. The PID controller iteration downward continuation method is proposed. Based on the original continuation iteration method, the differential coefficient and integral coefficient are added. The result shows that the new method remarkably decreases the iteration number and the accuracy are verified by comparison with the numerical integration solution. The PID controller iteration downward continuation method is applied to the interpretation of GATEM data. For synthetic data, the after continuation interpretation results are closer to the true model than the z = 30 m interpretation results. The method is also applied to GATEM field data in Yangquan City, Shanxi Province, China. The interpretation performs using PID controller iteration downward continuation results in a reliable GATEM field.