bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of immune-related therapeutically relevant biomarkers in breast cancer and breast cancer stem cells by transcription-wide analysis: a clinical prospective study
Authors: Wang Linbang , Wang Yuanyuan , Liu Jingkun , Tai Jiaojiao , Yin Xuedong , Liu Wei 
Published: April 2020

Abstract
Background: Cancer stem cells (CSCs) represent a subset of tumor cells that are responsible for recurrence and metastasis of tumors. These cells are resistant to radiotherapy and chemotherapy. Immunotherapeutic strategies that target CSCs specifically have provided initial results; however, the mechanism of action of these strategies is unclear. Methods: The data were requested from The Cancer Genome Atlas and Genotype-Tissue Expression, followed with the survival analysis and weighted gene co-expression network analysis to detect survival- and stemness-related genes. Patients were divided into three groups based on their immune status by applying ssGSEA with proven dependability by ESTIMATE analysis. The filtered key genes were analyzed using oncomine, qRT-PCR, and functional analysis. Results: Patients in a group with a higher stemness and a lower immune infiltration showed a worse overall survival probability, stemness and immune infiltration characteristics of breast cancer progressed in a non-linear fashion. Thirteen key genes related to stemness and immunity were identified and the functional analysis indicated their crucial roles in cell proliferation and immune escape strategies. The qRT-PCR results showed that the expression of PIMREG and MTFR2 differed in different stages of patients. Conclusions: Our study revealed a promising potential for CSC-target immunotherapy in the early stage of cancer and a probable value for PIMREG and MTFR2 as biomarkers and targets for immunotherapy. Trial registration: This study protocol registered with Chinese Clinical Trial Registry (http://www.chictr.org.cn/showproj.aspx?proj=19710; Date of registration: 25/09/2017; Registration number: ChiCTR-PDN-17012784) and was approved by the ethical committee of Affiliated Hospital of Chongqing Medical University (approval number: 2020-119). Keywords: breast cancer, cancer stem cell, tumor immune infiltration, PIMREG, MTFR2.
A systematic review and meta-analysis of the association between integrated tuberculosis and Human Immuno-deficiency Virus therapy and tuberculosis treatment outcomes among adults in sub-Saharan Africa
Authors: Kadia Benjamin Momo , Takah Noah Fongwen , Dimala Christian Akem , Simms Victoria 
Published: April 2020

Abstract
Background: Despite the scale-up of programmes integrating treatment of tuberculosis (TB) and Human Immuno-deficiency Virus (HIV) in sub-Saharan Africa (SSA), TB treatment outcomes in TB/HIV co-infected patients in the region remain sub-optimal.Objective: To summarize the available evidence on the association between integrated TB/HIV treatment and TB treatment outcomes specifically, successful treatment and all-cause mortality in TB/HIV co-infected adults in SSA.Method: A systematic review of studies published between March 2004 and 10 July 2019 was performed. Seven electronic databases including Medline, Embase and Cochrane were searched to identify interventional and observational quantitative studies reporting on integrated TB/HIV treatment in SSA. Two investigators independently screened the search output, reviewed the eligible studies, and rated the quality of eligible studies using quality assessment tools of the National Heart Lung and Blood Institute. Pooled odds ratios (ORs) were derived using random-effects meta-analysis. Heterogeneity across studies was assessed using the I2 statistic. The confidence in the pooled ORs was rated using the GRADE. The final review was reported using the PRISMA.Results: Eleven studies including 4181 participants were retained. The studies were of moderate to good quality, with 10 being quasi-experimental and cohort. Pooling of relevant studies showed that the odds of treatment success with integration was 1.1 times (95% CI: 0.93-1.29; I2=74.5%; p-value for heterogeneity=0.001) the odds of treatment success without integration and the odds of mortality with integration was 1.27 (95% CI 1.02-1.59; I2=87.3%; p-value for heterogeneity<0.0001) times the odds of mortality without integration. On sensitivity analysis, the odds of mortality with integration decreased to 1.06 (95% CI: 0.83-1.34; I2=80.1%; p-value for heterogeneity<0.0001) times the odds of mortality without integration and there was strong evidence of an association between sample size variation and heterogeneity (p=0.01). Good quality studies (4/11) tended to support the effectiveness of integrated treatment in increasing successful outcomes and decreasing mortality. Certainty in the pooled ORs was low.Conclusion: Evidence on the effect of integrated TB/HIV treatment services on treatment success and all-cause mortality in TB/HIV co-infected patients in SSA is inconclusive but the few available good quality studies tend to favour the effectiveness of these services. More robust primary studies are warranted.
Five-years Results of Small-incision Lenticule Extraction in High Myopia
Authors: Lang Min , Cao Kaiwei , Liu Ting , Pan Jiao , Ye Jian 
Published: April 2020

Abstract
Background To investigate long-term refractive visual quality outcomes and vision-related quality of life after small-incision lenticule extraction (SMILE) in the treatment of high myopia.Methods Thirty patients (60 eyes) with high myopia who underwent SMILE more than 5 years previously were selected as the SMILE group. Another 30 high myopia patients (60 eyes) who had worn corrective spectacles for more than 5 years were selected as the control group. In the SMILE group, the postoperative follow-up times were 3 months, 6 months, 1 year and 5 years. The uncorrected distance visual acuity (UDVA), corrected distance visual acuity (CDVA), spherical equivalent (SE), and ocular axial length (AL) were analysed. The Chinese version of the National Eye Institute Visual Function Questionnaire-25 (CHI-NEI-VFQ-25) was used to evaluate the vision-related quality of life in the SMILE group and the control group.Results In the SMILE group, the mean preoperative SE was -7.29±0.87 D (range -6.00 to -9.125 D). The efficacy index and safety index of SMILE were 1.09±0.18 and 1.19±0.12, respectively. Five years postoperatively, 44 eyes (73%) obtained a visual acuity of 20/20 or better. There were no eyes with CDVA loss of one or more Snellen lines. Forty-nine eyes (82%) and 57 eyes (95%) were within ±0.50 D and ±1.00 D of attempted correction, respectively. Forty-eight eyes (80%) had astigmatism < 0.50 D. The postoperative mean SE values at 3 months, 6 months, 1 year, and 5 years were 0.11±0.44 D, 0.07±0.45 D, -0.02±0.41 D, and -0.15±0.46 D, respectively. No significant change was observed in the ocular axial length from before operation to 5 years postoperatively (26.08±0.96 mm vs 26.01±0.94 mm, p>0.05). Compared to the control group, the SMILE group showed a significantly higher total score on the CHI-NEI-VFQ-25 (90.14 vs 81.43, p < 0.001).Conclusions In the present study, in a long-term follow-up, we demonstrated that correcting high myopia with SMILE is safe, effective, and predictable. In addition, there is slight undercorrection.
Chest radiography to assess and prognosticate COVID-19
Authors: Hui Terrence Chi Hong , Khoo Hau Wei , Young Barnaby Edward , Ong Sean Wei Xiang , Mohideen Salahudeen Mohamed Haja , Kalimuddin Shirin , Low Jenny Guek-Hong , Liew Charlene Jin Yee , Tan Seow Yen , Kok Shawn Shi Xian , Loh Jiashen , Chan Lai Peng , Poh Angeline Choo Choo , Wong Steven Bak Siew , Lim Chien Joo , Lin Raymond Tzer Pin , Leo Yee-Sin , Kaw Gregory Jon Leng , Lye David Chien , Tan Cher Heng 
Published: April 2020

Abstract
Background To determine the utility of chest radiography (CXR) for assessing and prognosticating COVID-19 disease with an objective radiographic scoring system.Methods A multicenter, prospective study was conducted, forty patients were included. Seventy-eight CXR’s were performed on the first derivation cohort of twenty patients with COVID-19 (median age 47.5 years, 10 females and four with comorbidities) admitted between 22 January 2020 and 1 February 2020. Each CXR was scored by three radiologists in consensus and graded on a 72-point COVID-19 Radiographic Score (CRS). This was correlated with supplemental oxygen requirement, C-reactive protein (CRP), lactate dehydrogenase (LDH) and lymphocyte count. To validate our findings, the parameters of another validation cohort of twenty patients with 65 CXRs were analysed.Results In the derivation cohort, seven patients needed supplemental oxygen and one was intubated for mechanical ventilation with no death. The maximum CRS was significantly different between patients on and not on supplemental oxygen (p=<.001). There was strong correlation between maximum CRS and lowest oxygen saturation (r= -.849), maximum CRP (r= .832) and maximum LDH (r= .873). These findings were consistent in the validation cohort. An increment of 2 points in CRS had an accuracy of 0.938 with 100.0% sensitivity (95% CI 100.0-100.0) and 83.3% (95% CI 65.1-100.0) specificity in predicting supplemental oxygen requirement.Conclusion Using an objective scoring system (CRS), the degree of abnormalities on CXR correlates closely with known markers of disease severity. CRS may further be applied to predict patients who require oxygen supplementation during the course of their disease.
An Ensemble of Community Detection in Social Networks Using Clustering of Users Demographic and Topological Information
Authors: Rezaeipanah Amin , Ghanat Kambiz 
Published: April 2020

Background:
One of the great challenges in social network analysis is community detection.
Community is a group of users which have high intra connections and sparse inter connections.
Community detection or clustering reveals community structure of social networks and
hidden relationships among their constituents. Nowadays, many different methods are proposed
to detect community structures in social networks from different perspectives, but none of them
can be a constant winner. Therefore, an ensemble of different methods can potentially improve
the final result.

Methods:
In this paper, we present a framework for different methods to be combined for community
detection. This method is a combination of genetic algorithms, particle swarm optimization, kmeans
clustering and Louvain clustering algorithms. Our method uses topological and demographic
information to identify communities and can automatically determine the number of optimal communities.

Results and Conclusion:
Quantitative evaluations based on extensive experiments on Ego-
Facebook social network dataset reveals that the method presented in this study achieves favorable
results, which are quite superior to other relevant algorithms in the literature.
• Discovering relationships between individuals by analyzing social networks.
• Providing identifying communities algorithms based on different clustering methods.
• An ensemble of community detection consisting of GA, PSO, k-means and Louvain clustering.
• The proposed method is better than the TSA method at silhouette and modularity criterion.
Demographic information also relates to the profile of users and their shared tweets.
PENGARUH KOMUNIKASI, MOTIVASI, DAN LINGKUNGAN KERJA FISIK TERHADAP SEMANGAT KERJA KARYAWAN UD. SUKRI DANA ABADI PONOROGO
Authors: Sugara Franky , Adji Setyo , Chamidah Siti 
Published: April 2020

Penelitian ini bertujuan untuk menguji pengaruh Komunikasi, Motivasi, Dan Lingkungan Kerja Fisik Terhadap Semangat Kerja karyawan pada UD. Sukri Dana Abadi Ponorogo dengan regresi berganda sebagai alat analisisnya. Populasi penelitian ini adalah semua karyawan yang terdapat di perusahaan UD. Sukri Dana Abadi dengan jenis sampel yaitu sampel jenuh. Data dikumpulkan dengan metode kuesioner. Hasil penelitian menunjukkan secara bersamaan variabel komunikasi, motivasi, dan lingkungan kerja fisik berpengaruh signifikan terhadap semangat kerja. Secara parsial variabel independen komunikasi, motivasi, maupun lingkungan kerja fisik berpengaruh signifikan terhadap variabel dependen semangat kerja. Hasil penelitian menunjukkan variabel komunikasi adalah variabel paling dominan berpengaruh terhadap semangat kerja.Kata Kunci : Komunikasi, Motivasi, Lingkungan Kerja Fisik, Semangat Kerja
Bergson: duración psicológica y acto libre
Authors: Rodríguez Serón Alicia 
Published: April 2020

RESUMENLa duración ha sido una de las nociones que ha hecho célebre la filosofía bergsoniana y es, sin duda, su noción central. La encontramos presente ya en el Ensayo (1889) referida a la vida psicológica. Más tarde, en La evolución creadora (1907), Bergson la extiende a los seres vivos, a la evolución de las especies y, posteriormente, a todo el universo. Es pues, el tejido mismo de lo real. centrándonos en la duración "psicológica", este trabajo pretende mostrar la concepción bergsoniana del acto libre y la libertad que de ella se desprende. PALABRAS CLAVEDURACION-ACTO LIBRE-TIEMPO FISICO-CONSCIENCIAABSTRACTThe duration has been one of the concepts wich has made Bergson's philosophhy famous and it is undoubtly its key concept. We already find it present in Essai (1889) concerning psychological life, and later on, in L'evolution creatrice (1907). Bergson extends it to living beings, to the evolution of the species and still to whole universe. Therefore, it is the very "stuff" of reality. being imposible to deal with this concept under these aspects, in this paper, we will focus on the "psychological" duration, in order to demonstrate that this notion means a conception of the free act and the freedom concerned. KEYWORDSDURATION-FREE ACT-PHYSICAL TIMES-CONCIOUSNESS
Análise Comparativa de Apreensões de Animais Silvestres em Dois Estados da Região Amazônica (Comparative Analysis of the Apprehension of Wild Animals in Two States of the Amazon Region)
Authors: Mendes Fabrício Lemos de Siqueira , Simonian Lígia Terezinha Lopes , Mendonça Ygor de Siqueira Mendes 
Published: April 2020

O Brasil apresenta a maior biodiversidade do planeta. E, a Amazônia é a região que mais colabora para essas estatísticas. Quando se referem aos Animais Silvestres (AS), o interesse por esses datam desde a chegada dos portugueses no Brasil, divulgando para o mundo todo a exuberância que essa região possuía. Atualmente, os AS na Amazônia são muito requisitados na dieta alimentar dessa região, em decorrência da necessidade de ingestão de proteína. Além disso, a população apresenta o hábito de ter em posse AS como estimação. O objetivo deste artigo é de identificar quais AS foram apreendidos, nos estado do Pará e Amazonas, e de mostrar também a área de atuação do órgão competente que rege a temática. Para isso, foi requisitado aos IBAMA dos estados do Pará e Amazonas os Relatório de Auto de Infração, onde constam os AS apreendidos e as cidades onde houve autuações deste Instituto. Deste modo, os resultados apontam que no estado do Pará os AS mais apreendidos foram as aves e no Amazonas foram os répteis. Com relação as cidades de autuação, em todos os dois estados foram visitadas mais de 50%, porém essas visitas são esporádicas ao longo dos anos. Com isso, conclui-se que nos estados do Pará e Amazonas há apreensões por parte do IBAMA, mas que precisam ter maior frequência anual em suas visitas. A B S T R A C TBrazil has the greatest biodiversity in the world and Amazon is the region that more contributes for these statistics. When referring to Wild Animals (AS), the interest in these are from the arrival of Portuguese in Brazil, disclosing to world all exuberance of this region. Currently, the AS in Amazon are in the diet of the animals of this region due to the need of protein intake. Besides, the population has the habit to take possession of wild animals as pets. The purpose of this article is to identify which AS were seized in the State of Para and Amazonas. For this, was requested to the Ibama of the States of Pará and Amazonas the High Infringement Reports that contain the AS seized and the cities rated by this Institute. The results indicate that in the State of Pará the most AS seized were birds and in Amazon region were reptiles. . It was concluded that in the States of Para and Amazonas there are seizures by IBAMA, but they need higher annual frequency of visits. Key words: Wild animals, Amazon, Seizure, Ibama, Illegality.
Fogo e seus efeitos na qualidade do solo de pastagem (Fire and its effects on the quality of pasture)
Authors: Vieira Ana Camila , Melloni Rogério , Melloni Eliane Guimarães Pereira , Guimarães Mariana Carvalho , Freitas Mariana Santos , Pecoraro Danilo 
Published: April 2020

No sul de Minas Gerais, o manejo de pastagem utilizando o fogo é comum. Poucos trabalhos abordam o efeito do fogo na qualidade do solo por meio da associação de atributos físicos, químicos e microbiológicos. O objetivo deste estudo foi avaliar o efeito imediato do fogo sobre atributos físicos, químicos e microbiológicos de solo de pastagem, classificado como Argissolo Vermelho Amarelo, de modo a observar a sua atuação como indicadores do impacto desse manejo. Foram estudados dois ambientes, queimado e não queimado, os quais foram subdivididos em 5 parcelas cada, com amostragem de solo emtrês profundidades (0-5 cm, 5-10 cm e 10-20 cm), para avaliação dos atributos físicos (textura, DMG, DMP, índice de floculação, macro e microporosidade, porosidade total), químicos (fertilidade básica) e microbiológicos (atividade e biomassa microbianas, quociente metabólico, densidade total de celulolíticos, amonificantes, desnitrificantes, nitrificantes e bactérias diazotróficas rizosféricas). O fogo não promoveu efeito na estrutura do solo e nos grupos microbianos estudados. Porém, proporcionou, na superfície do solo, maiores teores de P e K, e impactos negativos na biomassa microbiana e quociente metabólico, considerados bons indicadores do efeito do fogo na pastagem. A B S T R A C T The pasture management using fire is common. Few studies address the effects of fire on soil quality through the combination of physical, chemical and microbiological attributes. The objective of this study was to evaluate the immediate effect of fire on physical, chemical and microbiological pasture soil, in the southeast of Minas Gerais, classified as Red Yellow Ultisol, in order to observe their performance as the impact of management indicators. Two environments were studied burned and unburned, which were divided into five portions each with soil sampling at three depths (0-5 cm, 5-10 cm and 10-20 cm) for evaluation of physical attributes (texture, DMG, DMP, flocculation index, macro and microporosity, total porosity), chemical (basic fertility) and microbiological (activity and microbial biomass, metabolic quotient, total density of cellulolytic, ammonifiers, denitrifiers, nitrifiers and rhizospheric diazotrophic bacteria). The fire did not cause effect on soil structure and microbial groups studied. However, it provided, on the soil surface, higher levels of P and K, and negative impacts on microbial biomass and metabolic quotient, considered good indicators of the effect of fire on pasture. Key-words: Burning, Pasture, Indicator, Soil microbiology.
Uso e Ocupação do Solo na Reserva Faunística Costeira de Tibau do Sul (REFAUTS), Rio Grande do Norte, Brasil (1984 – 2015) (Land use and occupation at Coastal Wildlife Reserve of Tibau do Sul (REFAUTS), Rio Grande do Norte, Brazil (1984 – 2015))
Authors: De Freitas Diana Carvalho , Da Silva Paulo César Moura , Lunardi Vitor de Oliveira , Dos Santos Josivânia Emanuelly Azevedo , Lunardi Diana Gonçalves 
Published: April 2020

A Reserva Faunística Costeira de Tibau do Sul (REFAUTS), praia de Pipa, RN, vem sofrendo transformações quanto ao uso e ocupação do solo, como consequência do crescimento turístico na região. Assim, o objetivo deste estudo foi avaliar o uso e ocupação da REFAUTS ao longo de quatro décadas. Foram utilizadas quatro imagens de satélite dos anos 1984, 1994, 2004 e 2015. Estas foram classificadas tematicamente de acordo com categorias predeterminadas (água, vegetação arbórea, vegetação herbácea e edificações/solo exposto). Adicionalmente, foram registrados os empreendimentos turísticos na Zona de Uso Restrito e Controlado da Reserva. A partir da análise temporal, verificou-se que a REFAUTS, com apenas 555 km² de área total, sofreu impactos ambientais negativos. De 1984 a 2015, a porcentagem de área ocupada por vegetação arbórea foi reduzida em 0,57% e por vegetação herbácea, 4,33%. Entretanto, a área ocupada por edificações/solo exposto aumentou em 4,49%. Foram registrados em 2015 cerca de 29 empreendimentos comerciais operando na Zona de Uso Restrito e Controlado da Reserva. Dessa forma, torna-se necessário o ordenamento do uso e ocupação do solo na REFAUTS, que possui áreas frágeis como falésias, e ecologicamente relevantes, como remanescentes de restinga e Mata Atlântica. A B S T R A C T The Coastal Fauna Reserve of Tibau do Sul (REFAUTS), Pipa beach, RN, has been suffering transformations in the use and occupation of the soil, as a consequence of the tourist growth in the region. Thus, the objective of this study was to evaluate the use and occupation of REFAUTS over four decades. We used four satellite images from the years 1984, 1994, 2004 and 2015. These images were thematically classified according to predetermined categories (water, tree vegetation, herbaceous vegetation and buildings/ exposed soil). Additionally, we recorded commercial enterprise in Restricted and Controlled Use Zone of the Reserve. From the temporal analysis, we recorded that REFAUTS, with only 555 km2 of total area, suffered negative environmental impacts. From 1984 to 2015, the percentage of area occupied by tree vegetation was reduced by 0.57% and by herbaceous vegetation, 4.33%. However, the area occupied by buildings/exposed soil increased by 4.49%. We registered in 2015 about 29 commercial enterprise operating in the Restricted and Controlled Use Zone of the Reserve. Thus, it is necessary to organize the use and occupation of the soil in the REFAUTS, which has fragile areas such as cliffs, and ecologically relevant, such as remnants of restinga and Mata Atlântica. Keywords: Guiana dolphin; Pipa; environmental planning; Sotalia guianensis.