bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genome-wide identification and expression analysis of the CBF/DREB1 gene family in lettuce
Authors: Park Sunchung , Shi Ainong , Mou Beiquan 
Published: March 2020

AbstractThe C-repeat binding factor (CBF)/dehydration-responsive element binding (DREB1) proteins play a prominent role in freezing tolerance and are highly conserved in higher plants. Here we performed a genome-wide search of the CBF/DREB1 gene family in lettuce (Lactuca sativa L.) and identified 14 members of the family with one member gene containing a non-sense mutation within the AP2 DNA-binding domain. A comprehensive phylogenetic analysis of the CBF/DREB1 family members in 20 plant species from the Asterid or Rosid clade provided evidence that tandem duplication played an important role in the expansion of the CBF/DREB1 family. Expression analysis showed that twelve of the lettuce CBF genes were responsive to low temperature (4 °C), and that three and six of them could also be responsive to salt and heat stresses, respectively. Unlike Arabidopsis thaliana whose members of the CBF/DREB1 family respond only to a particular stress, lettuce CBFs provide wider protection from combinations of abiotic stresses. A global transcriptome analysis revealed distinctive temporal expression patterns among the cold-regulated genes in lettuce plants exposed to low temperature. Genes induced throughout the cold treatment are enriched in functions associated with protection from UV and high-light intensity and the genes suppressed after 7 days of cold exposure are enriched in photosynthesis-associated functions. These results provide insight into the molecular evolutionary properties of the CBF/DREB1 gene family in lettuce and a reference for genetic improvement of the lettuce response to cold acclimation.
Characterisation of the dynamic nature of lipids throughout the lifespan of genetically identical female and male Daphnia magna
Authors: Constantinou Julia K. , Southam Andrew D. , Kvist Jouni , Jones Martin R. , Viant Mark R. , Mirbahai Leda 
Published: March 2020

AbstractLipids play a significant role in regulation of health and disease. To enhance our understanding of the role of lipids in regulation of lifespan and healthspan additional studies are required. Here, UHPLC-MS/MS lipidomics was used to measure dynamic changes in lipid composition as a function of age and gender in genetically identical male and female Daphnia magna with different average lifespans. We demonstrate statistically significant age-related changes in triglycerides (TG), diglycerides (DG), phosphatidylcholine, phosphatidylethanolamine, ceramide and sphingomyelin lipid groups, for example, in males, 17.04% of TG lipid species decline with age whilst 37.86% increase in relative intensity with age. In females, 23.16% decrease and 25.31% increase in relative intensity with age. Most interestingly, the rate and direction of change can differ between genetically identical female and male Daphnia magna, which could be the cause and/or the consequence of the different average lifespans between the two genetically identical genders. This study provides a benchmark dataset to understand how lipids alter as a function of age in genetically identical female and male species with different average lifespan and ageing rate.
Antimicrobial and anti-inflammatory potential of Angelica dahurica and Rheum officinale extract accelerates wound healing in Staphylococcus aureus-infected wounds
Authors: Yang Wan-Ting , Ke Chun-Yen , Wu Wen-Tien , Tseng Yi-Hsiung , Lee Ru-Ping 
Published: March 2020

AbstractWound infection is a serious clinical problem, and the most common infection-causing bacteria are Staphylococcus aureus and Pseudomonas aeruginosa. Angelica dahurica and Rheum officinale extract (ARE) was reported to accelerate excisional wound healing in rats. In this study, we investigated the therapeutic effects of ARE on bacterial-infected wounds. Thirty Sprague-Dawley rats were divided into three groups: normal saline (NS), ARE, and biomycin ointment (BO). Full-thickness dorsal excisions in all the rats were infected with 108 colony-forming units of S. aureus; the treatments were applied once daily for 7 days. Results showed that the residual wound area in ARE group was smaller than those in NS and BO groups. TBCs on wound sites gradually decreased in ARE and BO groups. The body temperature and plasma inflammatory cytokines (TNF-α, IL-6) levels increased after bacterial infection at 24 h in all groups. After treatment, BT and inflammatory cytokines levels decreased in ARE group. Histological observations showed ARE group exhibited earlier scab formation, denser dermal granulation tissue, thicker epidermis, and more angiogenesis markers than the other groups. In conclusion, ARE accelerated wound healing in S. aureus-infected wounds. We proposed ARE exhibited potential antimicrobial and anti-inflammatory effects and stimulated angiogenesis, thus improving healing in infected wounds.
Investigating LGALS3BP/90 K glycoprotein in the cerebrospinal fluid of patients with neurological diseases
Authors: Costa Júlia , Pronto-Laborinho Ana , Pinto Susana , Gromicho Marta , Bonucci Sara , Tranfield Erin , Correia Catarina , Alexandre Bruno M. , de Carvalho Mamede 
Published: March 2020

AbstractGalectin-3 binding protein (LGALS3BP or 90 K) is a secreted glycoprotein found in human body fluids. Deregulated levels were observed in cancer and infection and its study in neurological diseases is more recent. Here, we have investigated 90 K from human cerebrospinal fluid (CSF) of patients with amyotrophic lateral sclerosis (ALS, n = 35) and other neurological diseases (n = 23). CSF was fractionated by ultrafiltration/size-exclusion chromatography (SEC) and eluted fractions were analysed by complementary techniques including immunoblotting, electron microscopy and nano-liquid chromatography-tandem mass spectrometry. A fraction of 90 K appeared as nanoparticles of irregular shape with heterogeneous dimensions of 15–60 nm that co-eluted with extracellular vesicles in SEC. Median levels of 90 K quantified by ELISA were not different between ALS patients (215.8 ng/ml) and controls (213.3 ng/ml) in contrast with the benchmark biomarker for ALS phosphoneurofilament heavy chain (1750 and 345 pg/ml, respectively). A multiregression model supported age is the only independent predictor of 90 K level in both groups (p < 0.05). Significant correlation was found between 90 K levels and age for the ALS group (r = 0.366, p = 0.031) and for all subjects (r = 0.392, p = 0.003). In conclusion, this study unveils the presence of 90 K-containing nanoparticles in human CSF and opens novel perspectives to further investigate 90 K as potential aging marker.
Scaling Behavior of Quasi-One-Dimensional Vortex Avalanches in Superconducting Films
Authors: Qviller A. J. , Qureishy T. , Xu Y. , Suo H. , Mozhaev P. B. , Hansen J. B. , Vestgården J. I. , Johansen T. H. , Mikheenko P. 
Published: March 2020

AbstractScaling behaviour of dynamically driven vortex avalanches in superconducting YBa2Cu3O7−δ films deposited on tilted crystalline substrates has been observed using quantitative magneto-optical imaging. Two films with different tilt angles are characterized by the probability distributions of avalanche size in terms of the number of moving vortices. It is found in both samples that these distributions follow power-laws over up to three decades, and have exponents ranging between 1.0 and 1.4. The distributions also show clear finite-size scaling, when the system size is defined by the depth of the flux penetration front – a signature of self-organized criticality. A scaling relation between the avalanche size exponent and the fractal dimension, previously derived theoretically from conservation of the number of magnetic vortices in the stationary state and shown in numerical simulations, is here shown to be satisfied also experimentally.
Duck plague virus gE serves essential functions during the virion final envelopment through influence capsids budding into the cytoplasmic vesicles
Authors: Liu Tian , Wang Mingshu , Cheng Anchun , Jia Renyong , Yang Qiao , Wu Ying , Liu Mafeng , Zhao Xinxin , Chen Shun , Zhang Shaqiu , Zhu Dekang , Tian Bin , Rehman Mujeeb Ur , Liu Yunya , Yu Yanling , Zhang Ling , Pan Leichang , Chen Xiaoyue 
Published: March 2020

AbstractDuck plague virus (DPV), a member of the alphaherpesviruses subfamily, causes massive ducks death and results in a devastating hit to duck industries in China. It is of great significance for us to analyze the functions of DPV genes for controlling the outbreak of duck plague. Thus, glycoproteins E (gE) of DPV, which requires viral cell-to-cell spreading and the final envelopment in herpes simplex virus 1 (HSV-1) and pseudorabies virus (PRV), was chosen herein. The gE mutant virus BAC-CHv-ΔgE was constructed by using a markerless two-step Red recombination system implemented on the DPV genome cloned into a bacterial artificial chromosome (BAC). Viral plaques on duck embryo fibroblast (DEF) cells of BAC-CHv-ΔgE were on average approximately 60% smaller than those produced by BAC-CHv virus. Viral replication kinetics showed that BAC-CHv-ΔgE grew to lower titers than BAC-CHv virus did in DEF cells. Electron microscopy confirmed that deleting of DPV gE resulted in a large number of capsids accumulating around vesicles and very few of them could bud into vesicles. The drastic inhibition of virion formation in the absence of the DPV gE indicated that it played an important role in virion morphogenesis before the final envelopment of intracytoplasmic nucleocapsids.
Western North Pacific Tropical Cyclone Activity in 2018: A Season of Extremes
Authors: Gao Si , Zhu Langfeng , Zhang Wei , Shen Xinyong 
Published: March 2020

AbstractThe 2018 tropical cyclone (TC) season over the western North Pacific (WNP) underwent two extreme situations: 18 TCs observed during June–August (JJA) and ranked the second most active summer in the satellite era; only 5 TCs that occurred during September–October (SO), making it the most inactive period since the late 1970s. Here we attribute the two extreme situations based on observational analyses and numerical experiments. The extremely active TC activity and northward shift of TC genesis during JJA of 2018 can be attributed to the WNP anomalous low-level cyclone, which is due primarily to El Niño Modoki and secondarily to the positive phase of the Pacific Meridional Mode (PMM). Overall, the extremely inactive TC activity during SO of 2018 is due to the absence of TC formation over the South China Sea and Philippine Sea, which can be attributed to the in-situ anomalous low-level anticyclone associated with the positive phase of the Indian Ocean Dipole, although the positive PMM phase and El Niño Modoki still hold.
Evaluation of Stratiolaelaps scimitus (Acari: Laelapidae) for controlling the root-knot nematode, Meloidogyne incognita (Tylenchida: Heteroderidae)
Authors: Yang Si-Hua , Wang Dan , Chen Chun , Xu Chun-Ling , Xie Hui 
Published: March 2020

AbstractRoot-knot nematodes are one of the most harmful plant-parasitic nematodes (PPNs). In this paper, the predation of Stratiolaelaps scimitus against Meloidogyne incognita was tested in an individual arena, and the control efficiency of the mite on the nematode in the water spinach (Ipomoea aquatica) rhizosphere was studied with a pot experiment. The results showed that S. scimitus could develop normally and complete its life cycle by feeding on second-stage juveniles of M. incognita (Mi-J2). The consumption rate of a 24 h starving female mite on Mi-J2 increased with the increase of prey density at 25 °C. Among the starvation treatments, the nematode consumption rate of a female mite starved for 96 h at 25 °C was highest; and among temperature treatments, the maximum consumption rate of a 24 h starving female mite on Mi-J2 was at 28 °C. The number of M. incognita in the spinach rhizosphere could be reduced effectively by releasing S. scimitus into rhizosphere soil, and 400 mites per pot was the optimum releasing density in which the numbers of root knots and egg masses decreased by 50.9% and 62.8%, respectively. Though we have gained a greater understanding of S. scimitus as a predator of M. incognita, the biocontrol of M. incognita using S. scimitus under field conditions remains unknown and requires further study.
Drug misuse, tobacco smoking, alcohol and other social determinants of tuberculosis in UK-born adults in England: a community-based case-control study
Authors: Nguipdop-Djomo Patrick , Rodrigues Laura C. , Smith Peter G. , Abubakar Ibrahim , Mangtani Punam 
Published: March 2020

AbstractAddressing social determinants of tuberculosis (TB) is essential to achieve elimination, including in low-incidence settings. We measured the association between socio-economic status and intermediate social determinants of health (SDHs, including drug misuse, tobacco smoking and alcohol), and TB, taking into account their clustering in individuals. We conducted a case-control study in 23–38 years old UK-born White adults with first tuberculosis episode, and randomly selected age and sex frequency-matched community controls. Data was collected on education, household overcrowding, tobacco smoking, alcohol and drugs use, and history of homelessness and prison. Analyses were done using logistic regression models, informed by a formal theoretical causal framework (Directed Acyclic Graph). 681 TB cases and 1183 controls were recruited. Tuberculosis odds were four times higher in subjects with education below GCSE O-levels, compared to higher education (OR = 3.94; 95%CI: 2.74, 5.67), after adjusting for other TB risk factors (age, sex, BCG-vaccination and stays ≥3 months in Africa/Asia). When simultaneously accounting for respective SDHs, higher tuberculosis risk was independently associated with tobacco smoking, drugs use (especially injectable drugs OR = 5.67; 95%CI: 2.68, 11.98), homelessness and area-level deprivation. Population Attributable Fraction estimates suggested that tobacco and class-A drug use were, respectively, responsible for 18% and 15% of TB cases in this group. Our findings suggest that socio-economic deprivation remains a driver of tuberculosis in England, including through drugs misuse, tobacco smoking, and homelessness. These findings further support the integration of health and social services in high-risk young adults to improve TB control efforts.
3D-Customized Guiding Template for Posterior Fixation in Complex Atlantoaxial Instability—Preliminary Experiences of National Cheng Kung University Hospital
Authors: Chen Yi-Yun , Chao Liang-Chun , Fang Jing-Jing , Lee E-Jian 
Published: March 2020

Objective Atlantoaxial fixation is technically demanding and challenging, especially in cases with anatomical abnormality. The purpose of this study is to report the effectiveness of the three-dimensional (3D)-customized guiding template for placement of C1 and C2 screws in cases with abnormalities.
Method Two patients with anatomical abnormality and one without were included. The preoperative computed tomography (CT) image was analyzed using our software. The entry point, trajectory, and depth of the screws were designed based on these images. Templates with screw guiding cylinders and cervical spine model were created. In operation, guiding templates were applied directly to the laminae. Drilling, tapping, and screwing were performed through the cylinders. To evaluate the accuracy, deviation of the screw axis from the preplanned trajectory was measured on postoperative CT. A classification system was taking to evaluate the pedicle screw insertion.
Results In complex cases, one of C2 screws has grade 2 deviation, and two has grade 1. There was no deviation in screws of C1. All patients achieved symptoms free after 6 months follow-up.
Conclusion Although 3D-printed template for atlantoaxial fixation still has limitation in complex cases, it has been proved usefulness and makes the most difficult and dangerous spinal posterior fixation easy to achieve.
C1 Hemilaminectomy for Resection of Foramen Magnum Meningioma: 2-Dimensional Operative Video
Authors: Lieber Stefan , Evangelista-Zamora Rocio , Nunez Maximiliano , Tatagiba Marcos 
Published: March 2020

AbstractWe present a case of a sizeable foramen magnum meningioma that was resected through a C1 hemilaminectomy in prone (concorde) position. The patient is a 51-year-old woman with a 3-month history of progressive paresthesia of the upper and lower extremities, followed by gait disturbance, and hand apraxia. There was no complaint of nuchal pain.On magnetic resonance imaging (MRI) a briskly enhancing extra-axial, intradural craniospinal lesion, extending from the basion of the lower clivus, over the tectorial membrane to the middle of the axis' body was discovered. There was significant transposition and compression of the medulla and corresponding focal hyperintensity on T2-weighted imaging.On physical examination, the patient was ambulatory independently, notwithstanding a pronounced spinal ataxia. There were deficits in sensation and proprioception, as well as urinary retention, but preserved function of the lower cranial nerves.In view of the profound transposition of the medulla, utilization of the corridor created by the tumor seemed feasible and we felt that a limited C1 hemilaminectomy would provide sufficient microsurgical access thus obviating a more extensive and invasive approach to the craniocervical junction.A gross-total resection was achieved; histopathology confirmed a World Health Organization (WHO) grade I angiomatous meningioma with a low-proliferation index. The patient was discharged home 3 days after surgery and her spinal ataxia resolved completely within 3 months of out-patient rehabilitation. At 3-year follow-up, there was no indication of residual or recurrence.The link to the video can be found at: https://youtu.be/WyShbfr-xi0.
Anatomic Variability of the Morphometric Parameters of the Third Ventricle of the Brain and Its Relations to the Shape of the Skull
Authors: Zhuravlova Iuliia , Kornieieva Maryna 
Published: March 2020

Abstract
Objective The defining of the normal parameters of spacious relations and symmetry of the ventricular system of the brain depending on the gender and age is currently one of the topical research problems of clinical anatomy. The present research aims to identify the correlation between the morphometric parameters of the third ventricle of the brain and the shape of the skull in the middle aged people.
Design This is a prospective cohort study.
Setting This study was set at the Trinity School of Medicine.
Participants A total of 118 normal computed tomography scans of the head of people aged from 21 to 86 years (mean age: 48.6 years ± 17.57) were selected for the study.
Main Outcome Measures The anteroposterior diameter, transverse diameter, and height of the third ventricle were measured and compared in dolichocranial, mesocranial, and brachycranial individuals.
Results The study has shown the presence of a statistically significant difference between morphometric parameters of the third ventricle of the brain in dolichocranial, mesocranial, and brachycranial individuals.
Conclusion The morphometric parameters of the third ventricle of the brain, such as height, anteroposterior diameter, and transverse diameter, depend on the individual anatomic variability of the skull shape and gender.
Endoscope-Assisted Middle Fossa Approach: Optimizing the Surgical Corridor for the Resection of Multicompartmental Chordomas
Authors: Beer-Furlan André , Vellutini Eduardo de Arnaldo Silva , Balsalobre Leonardo , Stamm Aldo Cassol 
Published: March 2020

Abstract
Background Skull base chordomas are a major therapeutic challenge. The surgical management involves selecting an approach that will offer the patient the best chance of largest/complete removal while minimizing morbidity and mortality.
Methods Medical records and imaging review of two skull base chordomas involving the middle fossa and posterior fossa that were successfully treated with an endoscope-assisted middle fossa approach.
Results The use of angled endoscopes provided better identification of anatomical landmarks and improved tumor resection when compared with the microscopic surgical exposure. The approach selection, anatomical landmarks, and technical aspects of the intraoperative setting of the endoscope-assisted approach are discussed.
Conclusion Endoscopic assistance in the middle fossa approach is a safe and valuable tool for maximizing the reach of the surgical corridor when treating skull base chordomas.
Laparoscopic Removal of Modified Vertical Uterine Compression Sutures due to Postoperative Focal Pain
Authors: Suzuki Toshifumi , Takeda Jun , Jinushi Makoto , Seyama Rie , Maruyama Yojiro , Makino Shintaro , Kitade Mari , Itakura Atsuo 
Published: March 2020

AbstractPreviously we reported laparoscopic removal of compression sutures due to uterine ischemia and related pain, which has two of the difficult aspects: (1) maneuvering the curved needle to perform compression suturing in the narrow surgical field, and (2) distinguishing between the threads of the cesarean section wound sutures versus the vertical compression sutures during removal, as the threads are the same white color. We performed vertical compression sutures for intrapartum hemorrhage with total placental previa, and modified both the needle type and the color of the thread used for uterine compression sutures during cesarean section. After the operation, we performed successful laparoscopic removal of compression sutures for postoperative focal pain. Changing the needle type and color helped to perform operations. The present case supports the concept that the laparoscopic removal of uterine compression suturing is useful for controlling pain in cases where general analgesics are ineffective.
Alkali magmatism on a carbonaceous chondrite planetesimal
Authors: Aléon Jérôme , Aléon-Toppani Alice , Platevoet Bernard , Bardintzeff Jacques-Marie , McKeegan Kevin D. , Brisset François 
Published: March 2020

SignificanceIn spite of magnetic and isotopic evidence revealing connections between the most primitive outer Solar System materials and differentiated bodies with a layered structure akin to terrestrial planets, the meteoritic record lacks samples that document the initiation of partial melting and the onset of planetary differentiation. Here, we describe a trachybasalt formed by very limited partial melting of a carbonaceous chondrite and crystallized as a dyke during ascent through the chondritic crust. This sample testifies of partial differentiation of outer Solar System carbonaceous planetesimals and can be considered as the long-sought first melt produced in differentiating small bodies.
The positioning of English-medium of instruction
Authors: Trent John 
Published: March 2020

Abstract
The proliferation of English-medium instruction (EMI) in higher education institutions (HEIs) across
non-English-speaking Europe has been recently documented in several large-scale surveys. The opportunities and challenges of
designing and implementing EMI policies are also widely recognized. However, our understanding of the use of EMI in Russian HEIs
is limited. This study responds to this research need by exploring the experiences and perspectives of instructors teaching
business-related subjects using the English language in two different Russian HEIs. A contribution of the study is to investigate
these perspectives and experiences using the analytic lens of positioning theory. Results reveal the ways in which instructors are
positioned by the university, as well how they position themselves, within an EMI environment. Acknowledging the potential
antagonism that might result between different EMI stakeholders because of this positioning and repositioning, suggestions are
then made as how this outcome could be avoided. Implications for future research are also considered.