bluuebunny/crossref_metadata_2025_split huggingface dataset:
Harnessing Expressed Single Nucleotide Variation and Single Cell RNA Sequencing To Define Immune Cell Chimerism in the Rejecting Kidney Transplant
Authors: Malone Andrew F. , Wu Haojia , Fronick Catrina , Fulton Robert , Gaut Joseph P. , Humphreys Benjamin D. 
Published: July 2020

Significance Statement
The combination of exome sequencing with single-cell RNA sequencing can reveal the recipient versus donor origin of each immune cell within human kidney allografts. This approach greatly improves upon previous techniques used to identify and describe leukocyte chimerism within a complex organ, such as Y chromosome identification for sex-mismatched transplants. Exome sequencing and single-cell RNA sequencing of single nucleotide variants indicated that donor-origin macrophages may contribute to the alloimmune response through antigen presentation and signaling, whereas donor-origin T cells remain quiescent. Therefore, teaming these techniques can paint a portrait of the chimerism that may lie behind rejection of a donor kidney.

Background
In solid organ transplantation, donor-derived immune cells are assumed to decline with time after surgery. Whether donor leukocytes persist within kidney transplants or play any role in rejection is unknown, however, in part because of limited techniques for distinguishing recipient from donor cells.

Methods
Whole-exome sequencing of donor and recipient DNA and single-cell RNA sequencing (scRNA-seq) of five human kidney transplant biopsy cores distinguished immune cell contributions from both participants. DNA-sequence comparisons used single nucleotide variants (SNVs) identified in the exome sequences across all samples.

Results
Analysis of expressed SNVs in the scRNA-seq data set distinguished recipient versus donor origin for all 81,139 cells examined. The leukocyte donor/recipient ratio varied with rejection status for macrophages and with time post-transplant for lymphocytes. Recipient macrophages displayed inflammatory activation whereas donor macrophages demonstrated antigen presentation and complement signaling. Recipient-origin T cells expressed cytotoxic and proinflammatory genes consistent with an effector cell phenotype, whereas donor-origin T cells appeared quiescent, expressing oxidative phosphorylation genes. Finally, both donor and recipient T cell clones within the rejecting kidney suggested lymphoid aggregation. The results indicate that donor-origin macrophages and T cells have distinct transcriptional profiles compared with their recipient counterparts, and that donor macrophages can persist for years post-transplantation.

Conclusions
Analysis of single nucleotide variants and their expression in single cells provides a powerful novel approach to accurately define leukocyte chimerism in a complex organ such as a transplanted kidney, coupled with the ability to examine transcriptional profiles at single-cell resolution.

Podcast
This article contains a podcast at https://www.asn-online.org/media/podcast/JASN/2020_08_07_JASN2020030326.mp3
The Capability Improvement Strategy of Cross-border E-commerce Supply Chain Service Providers
Authors: Xu Yi 
Published: July 2020

<p>In the development process of cross-border e-commerce, cross-border e-commerce supply chain providers play an essential role. Within their business scope, they are not good at cross-border logistics, overseas warehouses, information security in supply chain and other aspects. With the support of national policies, it is possible to innovate cross-border logistics service mode, improve the development ability of overseas warehouses, establish information supply chain partnership, attract or strengthen the training of cross-border e-commerce talents, so as to improve the ability.</p>
Hemicellulose Extraction and Characterization of Rice Straw and Leucaena Leucocephala
Authors: Nadiha Nor , . M. Z , B Jamilah 
Published: July 2020

Local production of agricultural waste is increasing. It is not fully utilized and can cause an environmental issue if it is not handle wisely. Thus, it is important to increase utilization of lignocellulosic biomass by improving their added value and subsequently decrease the agriculture waste. In this study, rice straw and Leucaena leucocephala were subjected to alkali treatment (4% sodium hydroxide) with different concentration ratio of samples to sodium hydroxide (1:10 to 1:50). The physical and chemical properties of extracted hemicelluloses were studied. The yield of hemicellulose was higher from rice straw compared to Leucaena leucocephala. The chemical functional groups present in hemicellulose were confirmed by Fourier tranform infrared spectroscopy (FTIR). The surface morphology and roughness of xylan were examined by scanning electron microscopy (SEM).
Keywords: rice straw, hemicellulose, sodium hydroxide, alkali
Knowledge, Acceptance and Application of Islamic Financial Management among Small and Medium Enterprises (SMEs) Halal Entrepreneurs in Peninsular of Malaysia
Authors: Daud Awang Mohd , Muhammad Junaina , Nur Aiman Mohd Noor Muhamad , Abdullah Amalina , Amir Abdullah Amini , Abdullah Norsazali , Shobihah Abang Abai Dayang 
Published: July 2020

The Halal industry in Malaysia has created employment opportunities especially among Small and Medium Enterprises (SMEs). High demand for Halal products especially from Islamic countries has transformed the development of this sector into a national priority. In the Eleventh Malaysia Plan (11MP), the competitiveness of the Halal industry was given special emphasis by the Prime Minister. In Asia, Malaysia has long been considered as a leader in the development of the Halal sector. However, the Halal industry is not only about Halal food production, but also covers Islamic finance services which prohibit interest (riba), uncertainties (gharar) and gambling (maysir). Islamic financial management is one of the important parts of Islamic finance because it plays a big role in providing Shariah-compliant funds and investment opportunities especially for the SME Halal entrepreneur. The holistic approach to the Halal industry should also cover not only food production but also Islamic financial management which includes activities such as how to create, increase, purify and to protect wealth from an Islamic perspective. Therefore, this study examines the relationship between the knowledge and acceptance of Islamic finance, and the application of Islamic financial management among halal entrepreneurs in Peninsular Malaysia. The findings of this study reveal that the knowledge and acceptance of Islamic finance is significantly correlated with the application of Islamic financial management among halal operators of SMEs. This indicates that to integrate the Halal industry with Islamic financial management activities, Halal entrepreneurs should be able to know and accept the Islamic finance principles. This will, in turn, facilitate the promotion of Malaysia’s Halal industries on the global stage.
Keywords: Halal Industry, SMEs Halal entrepreneur, Islamic Financial Management
Hermeneutica Hybrid Contract in Syaria Accounting Perspective
Authors: Handayati Puji , Prasetyo Aji 
Published: July 2020

The purpose of this study is to find out how the concept of hybrid contract is perceived amongst Muslim communities in Islamic banking. The hybrid contract concept was an innovative Islamic banking product designed to fulfill the needs of banking transactions in sharia banks. A transaction in a financial institution must not be separated from a set of financial statements in the accounting system. A Sharia-operated bank must apply the concept of sharia accounting, including accounting records of hybrid contract transactions. Using the qualitative method of hermeneutic phenomenology, this study concludes that in practice, the accounting records at the beginning of the transactions of “murabahah bil wakalah” (Syariah transaction) reflect directly on the ownership of the assets of murabaha by the bank. Whereas, before the asset belongs to the bank (which is represented by the customer), there is a previous contract that is “wakalah”. The occurrence of this wakalah contract is not included in accounting records. Therefore, if one only looks at the accounting records of a bank, it is impossible to gain a clear overview of a transaction with the agreement “murabahah bil wakalah”. Whereas, in accounting calculation using the “musyarakah mutanaqisah” contract, accounting treatment that acknowledged the financing of musyarakah, then in its presentation, there is certainly a Financial Position Report (balance sheet) showing the financing musyarakah as a whole.
Keywords: Hybrid Contract, Sharia Accounting, Accounting Recording
Educação popular e pedagogia decolonial: entrevista com Danilo Romeu Streck
Authors: Streck Danilo Romeu , Souza Tiago Zanquêta de , Novais Gercina Santana 
Published: July 2020

Entrevista com Danilo Romeu Streck, docente da Universidade do Vale do Rio dos Sinos (Unisinos) que recebeu em 2019 o Prêmio Pesquisador Gaúcho (destaque em Educação) da FAPERGS. O pesquisador realizou pós-doutorado na Universidade da Califórnia (EUA) e no Max-Planck Institut (Alemanha) e possui doutorado em educação pela State University of New Jersey (EUA). Streck coordena o grupo de pesquisa Mediações Pedagógicas e Cidadania e integrou o comitê assessor da área da educação do CNPq (2013-2018) e o comitê assessor da área da educação e psicologia da FAPERGS (2013-2016). É editor executivo do Journal of Action Research e professor colaborador no Programa de Doutorado Internacional em Pesquisa Ação na Universidade Sabanci em Istambul (Turquia). Seus temas de pesquisa são educação popular, educação e exclusão social, mediações pedagógicas e processos participativos.
Reaching training using sticky mittens as an early intervention protocol for an infant under environmental risk for motor delay: single-case research
Authors: Santana Camila A. S. , Greco Ana Luiza R. , Silva Carolina F. da , Tudella Eloisa 
Published: July 2020

BACKGROUND:Low socioeconomic status (low-SES) is an important environmental risk factor for developmental delay in infants. Access to affordable early intervention strategies may be limited for this population.As it is a low cost and easily reproducible intervention, reaching training using sticky mittens as a facilitator can be easily conducted by parents at home or in clinics, improving manual ability and exploration, and thus helping to avoid possible motor delays in infants from low-SES families.
AIM:To investigate the effects of reaching training using sticky mittens on manual reach and gross motor development of a 3 month-old full-term infant with low-SES.
METHOD:The training using sticky mittens occurred on 5 consecutive days, for 20 minutes per day.A total of 6 assessments were performed to measure: the number of reaches, proximal and distal adjustments (Kinovea), gross motor development (AIMS), and quality/quantity of affordances at home (AHEMD-IS).
RESULTS:After the training protocol, improved reaching ability was observed, with an increase in the number of reaches and bimanual adjustments, with hands open and verticalized. No improvements in other gross motor skills were observed.
CONCLUSION:Reaching training using sticky mittens seems to be a potential and affordable early intervention strategy for infants from low-SES families at risk for motor delay.
PENINGKATAN PEMBERDAYAAN KELOMPOK WANITA TANI DUSUN WONOREJO KOTA BATU MELALUI PELATIHAN PEMBUATAN PRODUK SUSU PROBIOTIK-SARI BUAH SEBAGAI MINUMAN KESEHATAN
Authors: Diyah Nuzul Wahyuning , Isnaeni Isnaeni , Darmawati Asri , Hardjono Suko 
Published: July 2020

Wonorejo village in Batu city is known as a vegetable and fruit producing area. Another popular product of Batu mostly is fresh dairy milk and only a small amount of processed products while the cow milk collected in KUD Batu has reached 16-17 tons per day. The problem was that the target community had no experiences in processing cow milk using probiotic microbes. The purpose was transfering of science and technology about the production of probiotic milk combined with fruit juice. This program was specifically directed to increase the empowerment of Kelompok Wanita Tani "Wonoasri" in Wonorejo, in developing of fruity- probiotic dairy products to increase the diversification of products with higher economic value. The method included workshop and technical assistance, which were carried out in a serie of steps: 1) exploring the knowledge of target by surveys and pre test, 2) lecturing and discussion, 3) demonstrating how to prepare and characterize the probiotic milk products, 4) training the preparation of product, 5) product characterization and packaging assistance, 6) evaluation. The results of this program were the target community gained increase in knowledge and experience, and they could produce fruity probiotic milk. In addition, we expected that they would increase their understanding and enthusiasm to appreciate the potency of their territory in order to improve the nation's competitiveness. abstrakDesa Wonorejo di kota Batu dikenal sebagai daerah penghasil sayur dan buah. Produk populer lain dari Batu adalah susu segar dan sejumlah kecil produk olahan. Susu sapi dikumpulkan di KUD Batu. Masalahnya adalah bahwa komunitas target tidak memiliki pengalaman dalam memproses susu sapi menggunakan mikroba probiotik. Tujuannya adalah mentransfer ilmu pengetahuan dan teknologi tentang produksi susu probiotik yang dikombinasikan dengan jus buah. Program ini diarahkan untuk pemberdayaan Kelompok Tani Wanita "Wonoasri" di Wonorejo, dalam mengembangkan produk susu buah-probiotik untuk diversifikasi produk dengan nilai ekonomi lebih tinggi. Metode tersebut termasuk lokakarya dan bantuan teknis, dalam serangkaian langkah: 1) mengeksplorasi pengetahuan target dengan survei dan pra-tes, 2) ceramah dan diskusi, 3) menunjukkan bagaimana mempersiapkan dan mengkarakterisasi produk susu probiotik, 4) pelatihan persiapan produk, 5) karakterisasi produk dan bantuan pengemasan, 6) evaluasi. Hasil program ini adalah komunitas sasaran memperoleh peningkatan dalam pengetahuan dan pengalaman, dan mereka dapat menghasilkan susu probiotik buah. Selain itu, kami berharap bahwa mereka akan meningkatkan pemahaman dan antusiasme mereka untuk menghargai potensi wilayah mereka untuk meningkatkan daya saing bangsa.
“COVID-19” MEME IN SOCIAL MEDIA: STUDY OF ROLAND BARTHES SEMIOLOGY
Authors: Isnaniah Siti , Tiya Agustina  
Published: July 2020

ABSTRACT
The objective of this research is to reveal the meaning of Covid-19 memes in social media using study of Roland Barthes semiology. In addition, this research also explains the social representation in Covid-19 memes. The method of the research is qualitative descriptive using technical analysis of Roland Barthes semiology. The technique of collecting data is based on two resources. The primary data are collections of memes about Covid 19 in some social media, such as Instagram, Facebook, and Web Blog. The secondary data are references supporting the topic of the research. In validity technique, the primary data are collected based on the topic of the discussion. After that, the data are processed descriptively by explaining, and comparing the information gained from many resources. Then, the data are reduced and presented. The result of the study shows that the creators tried to reveal social condition through Covid-19 memes. The social conditions were presented in some types of memes, namely critical memes, parodist memes, and motivational memes. In relation to meaning presentation, some social problems were revealed Covid-19 memes.
Keywords: Meme, Covid-19, Social Media, Roland Barthes Semiology
The State of Rural Public Health: Enduring Needs in a New Decade
Authors: Leider Jonathon P. , Meit Michael , McCullough J. Mac , Resnick Beth , Dekker Debra , Alfonso Y. Natalia , Bishai David 
Published: July 2020

Public health in the rural United States is a complex and underfunded enterprise. While urban–rural disparities have been a focus for researchers and policymakers alike for decades, inequalities continue to grow. Life expectancy at birth is now 1 to 2 years greater between wealthier urban and rural counties, and is as much as 5 years, on average, between wealthy and poor counties. This article explores the growth in these disparities over the past 40 years, with roots in structural, economic, and social spending differentials that have emerged or persisted over the same time period. Importantly, a focus on place-based disparities recognizes that the rural United States is not a monolith, with important geographic and cultural differences present regionally. We also focus on the challenges the rural governmental public health enterprise faces, the so-called “double disparity” of worse health outcomes and behaviors alongside modest investment in health departments compared with their nonrural peers. Finally, we offer 5 population-based “prescriptions” for supporting rural public health in the United States. These relate to greater investment and supporting rural advocacy to better address the needs of the rural United States in this new decade.
Cooperative Extension as a Force for Healthy, Rural Communities: Historical Perspectives and Future Directions
Authors: Buys David R. , Rennekamp Roger 
Published: July 2020

Cooperative Extension (Extension), part of the land-grant university system, has been engaged in rural communities for more than a century. While the focus of Extension’s efforts has largely centered on agriculture, there is an important thread of work that has similarities to public health.As Extension settles into its second century, we are working to be even more engaged in efforts that improve the health and well-being of rural communities in particular. Extension faculty and staff are accomplishing this through direct-to-the-population education and through partnerships with more classically oriented public health organizations able to leverage Extension’s networks and positive reputation in communities to engage them and improve their health. A component of these partnerships includes Extension faculty and staff increasingly engaging in policy, systems, and environment work and other initiatives that help ensure longer-term, systemic changes more likely to improve health outcomes.In short, Extension clearly changed the agricultural system of the United States, and because of its reach into rural communities, it has the capacity to do for health in rural communities in this second century what it did for agriculture in the first century.
Hepatitis A Outbreaks Associated With the Opioid Epidemic in Kentucky Counties, 2017–2018
Authors: DuPre Natalie , Blair Lyndsey , Moyer Sarah , Cook E. Francis , Little Bert , Howard Jeffrey 
Published: July 2020

Objectives. To describe county-level socioeconomic profiles associated with Kentucky’s 2017–2018 hepatitis A outbreak that predominately affected communities affected by the opioid epidemic. Methods. We linked county-level characteristics on socioeconomic and housing variables to counties’ hepatitis A rates. Principal component analysis identified county profiles of poverty, education, disability, income inequality, grandparent responsibility, residential instability, and marital status. We used Poisson regression to estimate adjusted relative risks (RRs) and 95% confidence intervals (CIs). Results. Counties with scores reflecting an extremely disadvantaged profile (RR = 1.21; 95% CI = 0.99, 1.48) and greater percentage of nonmarried men, residential instability, and income inequality (RR = 1.15; 95% CI = 0.94, 1.41) had higher hepatitis A rates. Counties with scores reflecting more married adults, residential stability, and lower income inequality despite disability, poverty, and low education (RR = 0.77; 95% CI = 0.59, 1.00) had lower hepatitis A rates. Counties with a higher percentage of workers in the manufacturing industry had slightly lower rates (RR = 0.97; 95% CI = 0.94, 1.00). Conclusions. As expected, impoverished counties had higher hepatitis A rates. Evaluation across the socioeconomic patterns highlighted community-level factors (e.g., residential instability, income inequality, and social structures) that can be collected to augment hepatitis A data surveillance and used to identify higher-risk communities for targeted immunizations.
Extra-Articular Hip Resection and Reconstruction with Custom Acetabular Resection Guide and Implants in a Case of High-Grade Spindle Cell Sarcoma of the Proximal Femur
Authors: Manrique Jorge , Schiffman Corey J. , Chan Albert D. , Olsen David , Thompson Matthew J. 
Published: July 2020

Case:
A 29-year-old man was admitted with acute atraumatic left hip pain and inability to bear weight. Subsequent workup revealed an intracapsular pathologic fracture of the femoral neck secondary to a high-grade spindle cell sarcoma. A unique method of extra-articular resection and reconstruction using a 3-dimensional (3D)-printed custom cutting jig and a custom acetabular component was pursued. Wide margins were achieved without violating the joint capsule but preserving pelvic integrity, allowing a return to an active lifestyle.

Conclusion:
Three-dimensional-printed custom resection guides and implants can help achieve adequate resection margins while preserving pelvic integrity and function.
Delayed Radial Nerve Palsy After Nonoperative Treatment of Humeral Shaft Fractures
Authors: Kelly Erin A. , Walden Timothy , Gross Jonathan , Egol Kenneth A. 
Published: July 2020

Case:
Two patients who developed radial nerve palsy at least 6 weeks after injury during nonoperative treatment of humeral shaft fractures. This complication was associated with external bracing, progressive varus angulation during treatment, and excess callus formation.

Conclusion:
Delayed radial nerve palsy may develop during nonoperative treatment of humeral shaft fractures when functional bracing fails to maintain alignment and stability at the fracture site.
Ambulatory blood pressure is better associated with target organ damage than clinic blood pressure in patients with primary glomerular disease
Authors: wen ruowei , chen xiaoqiu , zhu ye , ke jianting , du yi , wang cheng 
Published: July 2020

Abstract
Background: Blood pressure is an important and modifiable cardiovascular risk factor. Ambulatory blood pressure monitoring (ABPM) provides valuable prognostic information in patients with chronic kidney disease (CKD), yet little is known about the association of various types of BP measurements with target organ damage (TOD) in patients with primary glomerular disease. The goal of this study was to investigate whether ambulatory blood pressure is better associated with TOD than clinic blood pressure in patients with primary glomerular disease. Methods: 1178 patients with primary glomerular disease were recruited in this cross-sectional study. TOD were assessed by the following 4 parameters: left ventricular mass index (LVMI or LVH, left ventricular hypertrophy), estimated glomerular filtration rate (eGFR<60ml/min/1.73m 2 ), albumin-to-creatinine ratio (ACR≥30mg/g) and carotid intima-media thickness (cIMT) or plaque. Receiver operating characteristic (ROC) curve and multivariate logistic regression analyses were used to evaluate the relationship between ambulatory or clinic systolic blood pressure (SBP) indexes and TOD. Results: Among 1178 patients (mean age, 39 years,54% men), 116, 458, 1031 and 251 patients had LVH, eGFR < 60 ml/min/1.73m 2 , ACR≥30mg/g and cIMT≥0.9mm or plaque respectively. Area under ROC curves for TOD in ambulatory SBP, especially nighttime SBP, was greater than that in clinic SBP ( P <0.05). Multivariate logistic regression analyses showed that 24h SBP, daytime SBP and nighttime SBP were significantly associated with LVH, eGFR<60 ml/min/1.73m 2 and ACR≥30mg/g after adjustment for clinic SBP, while the association of clinic SBP was attenuated after further adjustment for nighttime SBP. Conclusions: Ambulatory blood pressure, especially nighttime blood pressure, is probably superior to clinic blood pressure in predicting TOD in patients with primary glomerular disease.