bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association of functional (GA)n microsatellite polymorphism in the FLI1 gene with susceptibility to human systemic sclerosis
Authors: Yamashita Keita , Kawasaki Aya , Matsushita Takashi , Furukawa Hiroshi , Kondo Yuya , Okiyama Naoko , Nagaoka Shouhei , Shimada Kota , Sugii Shoji , Katayama Masao , Hirohata Shunsei , Okamoto Akira , Chiba Noriyuki , Suematsu Eiichi , Setoguchi Keigo , Migita Kiyoshi , Sumida Takayuki , Tohma Shigeto , Hamaguchi Yasuhito , Hasegawa Minoru , Sato Shinichi , Kawaguchi Yasushi , Takehara Kazuhiko , Tsuchiya Naoyuki 
Published: May 2020

Abstract

Objective
Susceptibility genes that can account for characteristic features of SSc such as fibrosis, vasculopathy and autoimmunity remain to be determined. In mice, deficiency of Friend leukaemia integration 1 transcription factor (Fli1) causes SSc-like disease with these features. The human FLI1 gene contains (GA)n microsatellite, which has been shown to be associated with expression level. Because microsatellite polymorphisms are difficult to capture by genome-wide association studies, we directly genotyped FLI1 (GA)n microsatellite and examined its association with SSc.

Methods
Genomic DNA from 639 Japanese SSc patients and 851 healthy controls was genotyped for (GA)n microsatellite using the fragment assay. The cut-off repeat number for susceptibility to SSc was determined by receiver operating characteristics (ROC) analysis. Association with susceptibility and clinical characteristics was examined using logistic regression analysis. FLI1 mRNA levels were determined using quantitative RT-PCR.

Results
Based on the ROC analysis, (GA)n alleles with ≥22 repeats were collectively defined as L alleles and alleles with ≤21 repeats as S alleles. (GA)n L alleles were significantly associated with susceptibility to SSc (P = 5.0e-04, odds ratio 1.34, additive model). Significant association was observed both in diffuse cutaneous and limited cutaneous SSc. Among the SSc, (GA)n L alleles were significantly enriched in the patients with a modified Rodnan total skin thickness score ≥10 compared with those with a score &lt;10. FLI1 mRNA levels were significantly decreased in healthy controls carrying (GA)n L alleles as compared with non-carriers.

Conclusion
Extended repeat alleles of FLI1 (GA)n microsatellite may be associated with lower FLI1 mRNA levels and susceptibility to human SSc.
Palliative care, resuscitation status, and end-of-life considerations in pediatric anesthesia
Authors: Erondu Mgbechi U. , Mahoney Daniel P. 
Published: May 2020

Purpose of review
To familiarize pediatric anesthesiologists with primary palliative care procedural communication skills and recommendations for discussions involving complex medical decision-making or advance care planning, such as discussions about resuscitation status.

Recent findings
Recent publications highlight the benefits of pediatric palliative care (PPC) for seriously ill patients and their families, and how PPC principles might be applied to perioperative communication and decision-making. Both prospective and retrospective reports reveal improved quality of life, symptom management, and avoidance of unnecessary interventions when PPC is introduced early for a child with serious illness.

Summary
Pediatric anesthesiologists will, at some point, care for a child with serious illness who would benefit from PPC. It is important that all members of the perioperative care team are familiar with primary PPC procedural communication skills and models for approaching discussions about goals of care, shared decision-making, and advance care planning. Pediatric anesthesiologists should be incorporated as early as possible in team discussions about potential procedures requiring sedation for seriously ill children.
Conjoined twins in 2020 – state of the art and future directions
Authors: Frawley Geoff 
Published: May 2020

Purpose of review
A number of high profile conjoined twin separations have been extensively covered by the world media. Anaesthesia for conjoined twins is a procedure rarely experienced by paediatric anaesthetists. The increased survival of the twins has prompted discussion as to the most appropriate selection of patients, teams and hospitals to provide exceptional anaesthetic care.

Recent findings
The number of conjoined twins presenting for surgery remains low with many infants not surviving foetal or early neonatal life. Anaesthetic management of less common conjoined infants such as craniopagus twins has highlighted the benefit of careful patient selection, extensive preoperative investigations and meticulous multidisciplinary team planning. The role of simulation of possible adverse perioperative events has been highlighted. Three dimensional anatomical models and virtual reality systems have permitted surgical planning in advance of actual intervention. A number of legal and ethical concerns have been reported especially in the setting of emergency separation where surgery is likely to contribute to death of one of the twins.

Summary
There appears to be an expanding role for international teams with extensive separation experience becoming involved in international teleconferencing to improve patient management in low-resource countries. Whether the perioperative outcome is better when the conjoined twins are transferred to major centres for surgery or teams operate in the twin's country of origin remains to be seen.
Functional health and white matter hyperintensities as effect modifiers of blood pressure-lowering on cognitive function and vascular events in older Secondary Prevention of Small Subcortical Strokes trial participants
Authors: Blum Manuel R. , Scherzer Rebecca , Ikeme Jesse C. , Benavente Oscar R. , McClure Leslie A. , Peralta Carmen A. , Odden Michelle C. 
Published: May 2020

Objective:
To determine whether cerebral small vessel disease or disability modify the effect of SBP treatment on cognitive and vascular outcomes in older patients with recent lacunar stroke.

Methods:
Participants aged at least 65 years of the Secondary Prevention of Small Subcortical Strokes Trial were randomized to a higher (130–149 mmHg) or lower (<130 mmHg) SBP target. The primary outcome was change in cognitive function (Cognitive Abilities Screening Instrument); secondary outcomes were incident mild cognitive impairment, stroke, major vascular events (all-stroke, myocardial infarction), and all-cause death. Results were stratified by severity of white matter hyperintensities (WMH; none/mild, moderate, severe) on baseline MRI, and by disability (no vs. at least one limitation in activities of daily living).

Results:
One thousand, two hundred and sixty-three participants (mean age 73.8 ± 5.9 years, 40% women) were included. Participants with severe WMH or disability had worse cognitive function at baseline and after a mean follow-up of 3.9 years. No significant interactions existed between treatment group and effect modifiers (WMH, disability) for change in cognitive function (P for interaction 0.42 and 0.66, respectively). A lower SBP target appeared more beneficial among those with worse WMH burden for vascular outcomes (P for interaction = 0.01 for stroke and 0.03 for major vascular events).

Conclusion:
There was no difference in the effect of lowering SBP to less than 130 mmHg on cognitive function by cerebral small vessel disease or disability among older adults with a history of lacunar stroke. Those with evidence of small vessel disease may derive greater benefit from lower SBP on prevention of subsequent vascular events.

Trial registration:
Clinicaltrials.gov Identifier: NCT00059306.
Hypnosis in the clinical setting
Authors: Phillips Barbara C. 
Published: May 2020

ABSTRACT
Therapeutic hypnosis has been in use for hundreds of years, yet it is still is still relatively unknown as a therapeutic modality and underutilized by health care professionals. Nurse practitioners are in a unique position to bring hypnosis into the forefront of our work with patients and clients in most clinical settings. The author aims to introduce nurse practitioners to the potential and possibilities of incorporating hypnosis into individual treatment plans.
Pediatric Rib Fractures Identified by Chest Radiograph
Authors: Ruest Stephanie , Kanaan Ghid , Moore Jessica L. , Goldberg Amy P. 
Published: May 2020

ObjectiveThe objective of this study was to determine the prevalence of rib fractures (RFs) identified by chest x-ray (CXR) among children younger than 2 years who sustained accidental versus nonaccidental injuries. It is hypothesized that RFs are uncommon among all accidental pediatric trauma mechanisms (eg, falls, motor vehicle crashes) as compared with the prevalence of RFs in the setting of nonaccidental trauma (NAT).MethodsA retrospective chart review of sequential CXRs of children younger than 2 years evaluated at a pediatric level 1 trauma center for accidental trauma and possible NAT was conducted from January 1, 2011, to October 31, 2016. Data collected included demographics, CXR indication and findings, history of cardiopulmonary resuscitation, trauma mechanism, associated injuries, final diagnoses, and outcomes.ResultsTwo (<1%) of 226 CXRs obtained to evaluate accidental trauma demonstrated acute RFs. Ten (19.6%) of 51 CXRs obtained in the setting of concern for NAT revealed RFs (9/10 identified only healing RFs and 1/10 identified acute RFs). Among patients with a final diagnosis of NAT (ie, not neglect, accidental trauma, etc; n = 38), the overall prevalence increased to 26.3%.ConclusionsThe presence of RFs in pediatric accidental trauma is uncommon even in the setting of high-force mechanisms, and when identified, these RFs are acute. Comparatively, the overall prevalence of RFs identified on CXR among cases with a final diagnosis of NAT was much higher and almost exclusively healing RFs. These data provide support that identification of RFs is highly concerning for NAT even if an accidental mechanism is provided. When RFs are identified, a full NAT work-up should be considered.
The War on COVID-19 Pandemic
Authors: Lew Henry L. , Oh-Park Mooyeon , Cifu David X. 
Published: May 2020

Abstract
The global outbreak of coronavirus disease 2019 has created an unprecedented challenge to the society. Currently, the United States stands as the most affected country, and the entire healthcare system is affected, from emergency department, intensive care unit, postacute care, outpatient, to home care. Considering the debility, neurological, pulmonary, neuromuscular, and cognitive complications, rehabilitation professionals can play an important role in the recovery process for individuals with coronavirus disease 2019. Clinicians across the nation’s rehabilitation system have already begun working to initiate intensive care unit–based rehabilitation care and develop programs, settings, and specialized care to meet the short- and long-term needs of these individuals. We describe the anticipated rehabilitation demands and the strategies to meet the needs of this population. The complications from coronavirus disease 2019 can be reduced by (1) delivering interdisciplinary rehabilitation that is initiated early and continued throughout the acute hospital stay, (2) providing patient/family education for self-care after discharge from inpatient rehabilitation at either acute or subacute settings, and (3) continuing rehabilitation care in the outpatient setting and at home through ongoing therapy either in-person or via telehealth.
Quality and Safety in Healthcare, Part LXX
Authors: Harolds Jay A. 
Published: May 2020

Abstract
The chief medical officer ideally has a degree in business, hospital administration, or related subjects and has also pursued training in leadership. This is very important to be able to read and interpret financial documents, understand the language of hospital executives, and be able to advise the administrators and physicians on many projects. The chief medical officer must be trusted by both the C-suite executives and the medical staff and be able to communicate effectively and diplomatically with both. In order to optimize value, it is important to engage the medical staff and to align the goals of not only the executives and doctors, but also of the patients and their families.
Comparison of the Olfactory Functions in Conventional Endoscopic Sinus Surgery to the Balloon Sinuplasty in Frontal Sinus Surgery
Authors: Hanci Deniz , Gürpinar Berk , Üstün Onur , Kumral Tolgar Lütfi , Karaketir Semih , Uyar Yavuz 
Published: May 2020

Objective:
Balloon sinuplasty is being used worldwide, however the olfactory functions after balloon sinuplasty are underestimated. The authors aimed to study the effects of conventional endoscopic sinus surgery (ESS) compared to the balloon sinuplasty on olfactory function.

Materials and Methods:
Forty-four subjects were randomly divided into 2 groups as ESS and balloon sinuplasty. The olfactory functions of each subject were recorded preoperatively and at the 1st, 3rd, 6th, and 12th months postoperatively.

Results:
The initial assessments of olfaction were the same in both groups. After the surgery, the olfactory functions were significantly better in those of ESS group (P < 0.05).

Conclusion:
The olfactory function is preserved better in the conventional surgery compared to the balloon catheter dilation of the frontal sinus.
Risk Factors for Surgical Site Infection in Open and Laparoscopic Hartmann Closure: A Multivariate Analysis
Authors: Brathwaite Shayna , Latchana Nicholas , Esemuede Iyare , Harzman Alan , Husain Syed 
Published: May 2020

Background:
Hartmann reversal is a high-morbidity procedure. The aim of this study is to identify risk factors for surgical site infection (SSI) in the era of laparoscopy.

Materials and Methods:
A query of the National Surgical Quality Improvement Program database was done. Patients undergoing open or laparoscopic Hartmann reversals were identified. Risk factors for and the incidence of SSI were assessed in both groups.

Results:
A total of 7970 patients were identified and 1431 (18%) were done laparoscopically. The SSI rate in the overall population was 13.6%, with 14.9% in those undergoing open surgery and 8% with laparoscopic procedures. Obese patients and smokers had the highest incidences of SSI (18% and 17.5%, respectively). Open surgery (odds ratio=1.8, P<0.001) and obesity (odds ratio=1.6, P<0.001) significantly correlated with higher SSI rates.

Conclusions:
Patients undergoing Hartmann closure are at risk for SSI. Our findings indicate that laparoscopy can significantly reduce SSI, particularly in obese patients.
Weight Loss Outcomes in Laparoscopic Vertical Sleeve Gastrectomy (LVSG) Versus Laparoscopic Roux-en-Y Gastric Bypass (LRYGB) Procedures: A Meta-Analysis and Systematic Review of Randomized Controlled Trials
Authors: Osland Emma , Yunus Rossita M. , Khan Shahjahan , Memon Breda , Memon Muhammed A. 
Published: May 2020

Purpose:
Laparoscopic Roux-en-Y gastric bypass (LRYGB) and laparoscopic vertical sleeve gastrectomy (LVSG) have been proposed as cost-effective strategies to manage morbid obesity. The aim of this meta-analysis was to compare the postoperative weight loss outcomes reported in randomized control trials (RCTs) for LVSG versus LRYGB procedures.

Material and Methods:
RCTs comparing the weight loss outcomes following LVSG and LRYGB in adult population between January 2000 and November 2015 were selected from PubMed, Medline, Embase, Science Citation Index, Current Contents, and the Cochrane database. The review was prepared in accordance with Preferred Reporting of Systematic Reviews and Meta-Analyses (PRISMA).

Results:
Nine unique RCTs described over 10 publications involving a total of 865 patients (LVSG, n=437; LRYGB, n=428) were analyzed. Postoperative follow-up ranged from 3 months to 5 years. Twelve-month excess weight loss (EWL) for LVSG ranged from 69.7% to 83%, and for LRYGB, ranged from 60.5% to 86.4%. A number of studies reported slow weight gain between the second and third years of postoperative follow-up ranging from 1.4% to 4.2%EWL. This trend was seen to continue to 5 years postoperatively (8% to 10%EWL) for both procedures.

Conclusions:
In conclusion, LRYGB and LVSG are comparable with regards to the weight loss outcomes in the short term, with LRYGB achieving slightly greater weight loss. Slow weight recidivism is observed after the first postoperative year following both procedures. Long-term reporting of outcomes obtained from well-designed studies using intention-to-treat analyses are identified as a major gap in the literature at present.
Nocardioides dongxiaopingii sp. nov., isolated from leaves of Lamiophlomis rotata on the Qinghai–Tibet Plateau
Authors: Zhang Sihui , Wang Xiaoxia , Yang Jing , Lu Shan , Lai Xin-He , Jin Dong , Huang Yuyuan , Zhu Wentao , Li Junqin , Pu Ji , Huang Ying , Tian Zhi , Dong Kui , Zhang Gui , Lei Wenjing , Wang Suping , Xu Jianguo 
Published: May 2020

Two novel Gram-stain-positive, irregular rod-shaped actinomycetes, S-1144T and 4053, were isolated from leaves of Lamiophlomis rotata on the Qinghai–Tibet Plateau, PR China. Cells were aerobic, catalase-positive and oxidase-negative. Colonies on Reasoner’s 2A agar were light yellow, circular, shiny, smooth and convex after 2 days of incubation. The isolates grew optimally at 25 °C, pH 7.5 and with 0 % (w/v) NaCl. The results of polyphasic analyses indicated that strain S-1144T belonged to the genus

Nocardioides

and its close phylogenetic neighbours (16S rRNA gene sequence similarity) were

Nocardioides litoris

DSM 103718T (98.4 %),

Nocardioides rubriscoriae

DSM 23986T (98.2%) and

Nocardioides plantarum

DSM 11054T (97.8 %). The genome of strain S-1144T showed less than 70 % digital DNA–DNA hybridization and < 95–96 % average nucleotide identity values to the above reference strains. The DNA G+C content of strain S-1144T was 73.5 mol%. MK-8(H4) was the predominant respiratory quinone (96.0 %) and llLL-2,6-diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan. The polar lipid profile of strain S-1144T consisted of diphosphatidylglycerol, phosphatidylglycerol, three unidentified phospholipids, one unidentified glycolipid and one unidentified lipid. The major cellular fatty acids were iso-C16 : 0, C17 : 1
ω8c, C17 : 0 and C18 : 1
ω9c. On the basis of obtained data, strain S-1144T represented a novel species of the genus

Nocardioides

, for which the name Nocardioides dongxiaopingii sp. nov. is proposed. The type strain is S-1144T (=CGMCC 4.7568T=JCM 33469T).
Pseudoalteromonas rhizosphaerae sp. nov., a novel plant growth-promoting bacterium with potential use in phytoremediation
Authors: Navarro-Torre Salvadora , Carro Lorena , Rodríguez-Llorente Ignacio D. , Pajuelo Eloísa , Caviedes Miguel Ángel , Igual José Mariano , Klenk Hans-Peter , Montero-Calasanz Maria del Carmen 
Published: May 2020

Strain RA15T was isolated from the rhizosphere of the halophyte plant Arthrocnemum macrostachyum growing in the Odiel marshes (Huelva, Spain). RA15T cells were Gram stain-negative, non-spore-forming, aerobic rods and formed cream-coloured, opaque, mucoid, viscous, convex, irregular colonies with an undulate margin. Optimal growth conditions were observed on tryptic soy agar (TSA) plates supplemented with 2.5 % NaCl (w/v) at pH 7.0 and 28 °C, although it was able to grow at 4–32 °C and at pH values of 5.0–9.0. The NaCl tolerance range was from 0 to 15 %. The major respiratory quinone was Q8 but Q9 was also present. The most abundant fatty acids were summed feature 3 (C16 : 1
ω7c and/or C16 : 1
ω6c), C17 : 1
ω8c and C16 : 0. The polar lipids profile comprised phosphatidylglycerol and phosphatidylethanolamine as the most abundant representatives. Phylogenetic analyses confirmed the well-supported affiliation of strain RA15T within the genus

Pseudoalteromonas

, close to the type strains of

Pseudoalteromonas neustonica

,

Pseudoalteromonas prydzensis

and

Pseudoalteromonas mariniglutinosa

. Results of comparative phylogenetic and phenotypic studies between strain RA15T and its closest related species suggest that RA15T could be a new representative of the genus

Pseudoalteromonas

, for which the name Pseudoalteromonas rhizosphaerae sp. nov. is proposed. The type strain is RA15T (=CECT 9079T=LMG 29860T). The whole genome has 5.3 Mb and the G+C content is 40.4 mol%.
Chitinimonas arctica sp. nov., isolated from Arctic tundra soil
Authors: Xu Qiang , Jiang Fan , Da Xuyang , Zhang Yumin , Geng Yingchao , Qin Kun , Liu Jia , Peng Fang 
Published: May 2020

A Gram-stain-negative, rod-shaped, green-pigmented, aerobic and motile bacterium, strain R3-44T, was isolated from Arctic tundra soil. Stain R3-44T clustered closely with members of the genus

Chitinimonas

, which belongs to the family

Burkholderiaceae

, and showed the highest 16S rRNA sequence similarity to

Chitinimonas naiadis

AR2T (96.10%). Strain R3-44T grew optimally at pH 7.0, 28 °C and in the presence of 0–0.5 % (w/v) NaCl. The predominant respiratory isoprenoid quinone of strain R3-44T was identified as ubiquinone Q-8. The polar lipids consisted of phosphatidylglycerol, phosphatidylethanolamine, unidentified aminolipid and unidentified phospholipid. The main fatty acids were summed feature 3 (comprising C16 : 1
ω7c and/or C16 : 1
ω6c, 40.6 %) and C16 : 0 (29.3 %). The DNA G+C content of strain R3-44T was 60.8 mol%. On the basis of the evidence presented in this study, strain R3-44T represents a novel species of the genus

Chitinimonas

, for which the name Chitinimonas arctica sp. nov. is proposed, with the type strain R3-44T (=CCTCC AB 2010422T=KCTC 72602T).
Maribacter luteus sp. nov., a marine bacterium isolated from intertidal sand of the Yellow Sea
Authors: Liu Ang , Zhang Yan-Jiao , Liu De-Kun , Li Xiu-Zhen 
Published: May 2020

A novel rod-shaped and Gram-stain-negative bacterium, designated strain RZ05T, was isolated from a sand sample collected from the intertidal zone of the Yellow Sea, PR China. Results of phylogenetic analysis based on 16S rRNA gene sequences revealed that strain RZ05T clusters within the genus

Maribacter

, a member of the family

Flavobacteriaceae

, and has the highest sequence similarity to

Maribacter polysiphoniae

KCTC 22021T (97.8 %), followed by

Maribacter arenosus

KCTC 52191T (97.2 %). Cells of this strain were observed to be aerobic, oxidase- and catalase-positive, motile by gliding and formed yellow colonies. Growth occurred at 7–40 °C (optimum, 30 °C), at pH 6.5–9.5 (optimum, pH 7.0) and with 0.5–6 % (optimum, 2 %) NaCl. Its polar lipid profile included phosphatidylethanolamine, two unidentified glycolipids, one unidentified aminolipid and four unidentified lipids. The major cellular fatty acids were iso-C15 : 0, iso-C15 : 1 G, iso-C17 : 0 3-OH, iso-C16 : 0 3-OH, iso-C15 : 0 3-OH, summed feature 9 (10-methyl C16 : 0/iso-C17 : 1
ω9c) and summed feature 3 (iso-C15 : 0 2-OH/C16 : 1
ω7c/C16 : 1
ω6c). The only respiratory quinone was menaquinone 6 (MK-6). The genome of strain RZ05T was 4.65 Mbp with a G+C content of 38.9 mol%. The average nucleotide identity and in silico DNA–DNA hybridization values between strain RZ05T and its most closely related type strain

M. polysiphoniae

KCTC 22021T were 80.3 and 26.3  %, respectively. The results of phylogenetic, phenotypic and chemotaxonomic analyses indicated that strain RZ05T represents a novel species of the genus

Maribacter

, for which the name Maribacter luteus sp. nov. is proposed. The type strain is RZ05T (=KCTC 62834T=MCCC 1K03617T).