bluuebunny/crossref_metadata_2025_split huggingface dataset:
Adenovector 26 encoded prefusion conformation stabilized RSV-F protein induces long-lasting Th1-biased immunity in neonatal mice
Authors: van der Fits Leslie , Bolder Renske , Heemskerk-van der Meer Marjolein , Drijver Joke , van Polanen Yolinda , Serroyen Jan , Langedijk Johannes P. M. , Schuitemaker Hanneke , Saeland Eirikur , Zahn Roland 
Published: June 2020

AbstractWhile RSV is a major cause of respiratory morbidity in infants, vaccine development is hindered by the immaturity and Th2-bias of the infant immune system and the legacy of enhanced respiratory disease (ERD) after RSV infection following immunization with formalin inactivated (FI)-RSV vaccine in earlier clinical trials. Preclinical studies have demonstrated that an adenoviral vector-based RSV F vaccine candidate (Ad26.RSV.FA2) induces Th1-biased protective immune responses, without signs of ERD upon subsequent RSV challenge. We here developed an Ad26 vector encoding the RSV F protein stabilized in its prefusion conformation (Ad26.RSV.preF). In adult mice, Ad26.RSV.preF induced superior, Th1-biased IgG2a-dominated humoral responses as compared to Ad26.RSV.FA2, while maintaining the strong Th1-biased cellular responses. Similar to adult mice, Ad26.RSV.preF induced robust and durable humoral immunity in neonatal mice, again characterized by IgG2a-dominated RSV F-binding antibodies, and high and stable virus-neutralizing titers. In addition, vaccine-elicited cellular immune responses were durable and characterized by IFN-γ-producing CD4+ and CD8+ T cells, with a profound Th1 bias. In contrast, immunization of neonatal mice with FI-RSV resulted in IgG1 RSV F-binding antibodies associated with a Th2 phenotype, no detectable virus-neutralizing antibodies, and a Th2-biased cellular response. These results are supportive for the clinical development of Ad26.RSV.preF for use in infants.
Distribution and conservation of species is misestimated if biotic interactions are ignored: the case of the orchid Laelia speciosa
Authors: Flores-Tolentino Mayra , García-Valdés Raúl , Saénz-Romero Cuauhtémoc , Ávila-Díaz Irene , Paz Horacio , Lopez-Toledo Leonel 
Published: June 2020

AbstractThe geographic distribution of species depends on their relationships with climate and on the biotic interactions of the species. Ecological Niche Models (ENMs) mainly consider climatic variables only and may tend to overestimate these distributions, especially for species strongly restricted by biotic interactions. We identified the preference ofLaelia speciosafor different host tree species and include this information in an ENM. The effect of habitat loss and climate change on the distribution of these species was also estimated. AlthoughL. speciosawas recorded as epiphyte at six tree species, 96% of the individuals were registered at one single species (Quercus deserticola), which indicated a strong biotic interaction. We included the distribution of this host tree as a biotic variable in the ENM ofL. speciosa. The contemporary distribution ofL. speciosais 52,892 km2, which represent 4% of Mexican territory and only 0.6% of the distribution falls within protected areas. Habitat loss rate for L. speciosaduring the study period was 0.6% per year. Projections for 2050 and 2070 under optimistic and pessimistic climate change scenarios indicated a severe reduction in its distribution. Climaticaly suitable areas will also shift upwards (200–400 m higher). When estimating the distribution of a species, including its interactions can improve the performance of the ENMs, allowing for more accurate estimates of the actual distribution of the species, which in turn allows for better conservation strategies.
Genetic variations in catechol-O-methyltransferase gene are associated with levodopa response variability in Chinese patients with Parkinson’s disease
Authors: Zhao Cuiping , Wang Yihua , Zhang Bin , Yue Yaoxian , Zhang Jianyuan 
Published: June 2020

AbstractCatechol-O-methyltransferase (COMT) is one of the main enzymes in dopamine metabolism and is reported to be associated with susceptibility to Parkinson’s disease (PD) and pharmacotherapy. However, researchers mostly focus on the most common polymorphism, rs4680. In this case-control study, we investigated the association of SNPs other than rs4680 with the levodopa (L-dopa) response and other clinical features in Chinese PD patients. Eleven single nucleotide polymorphisms (SNPs) in the COMT gene were genotyped, and clinical data were collected. Patients with the TT genotype of rs165728 or rs174699 had larger daily levodopa equivalent doses (LEDs) than the patients with CC and CT genotypes under the dominant model (p = 0.01421 for rs165728 and p = 0.02302 for rs174699). Under the dominant model, the patients with GG at rs4680 G > A had a lower occurrence of dyskinesia than those with AA and AG (p = 0.0196). Patients with CC at rs4633 had a lower occurrence of dyskinesia than those with TT and TC (p = 0.0429) under the dominant model. The frequencies of the rs174675 T and rs933271 C alleles were higher in PD patients than in the controls (p < 0.05). Our primary results showed the possible association of SNPs other than the most common functional rs4680 in COMT with interindividual variance in the L-dopa daily dose and susceptibility to dyskinesia in Chinese patients, although this was an exploratory study based on a small sample size. Larger and more randomized samples are necessary for further investigation.
Identification of key genes and modules in response to Cadmium stress in different rice varieties and stem nodes by weighted gene co-expression network analysis
Authors: Wang Qi , Zeng Xiannan , Song Qiulai , Sun Yu , Feng Yanjiang , Lai Yongcai 
Published: June 2020

AbstractSoil cadmium (Cd) pollution threatens food safety. This study aimed to identify genes related to Cd accumulation in rice. Low- (Shennong 315, short for S315) and high- (Shendao 47, short for S47) Cd-accumulative rice cultivars were incubated with CdCl2·2.5H2O. RNA-seq and weighted gene co-expression network analysis (WGCNA) were performed to identify the modules and genes associated with Cd-accumulative traits of rice. After Cd stress treatment, the Cd content in various tissues of S315 was significantly higher than that of S47. In the stem nodes, the Cd distribution results of the two varieties indicated that the unelongated nodes near the root (short for node A) had a stronger ability to block Cd transfer upwards than the panicle node (short for node B). Cd stress induced huge changes in gene expression profiles. After analyzing the differentially expressed genes (DEGs) in significantly correlated WGCNA modules, we found that genes related to heavy metal transportation had higher expression levels in node A than that in node B, such as Copper transporter 6 (OS04G0415600), Zinc transporter 10 (OS06G0566300), and some heavy-metal associated proteins (OS11G0147500, OS03G0861400, and OS10G0506100). In the comparison results between S315 and S47, the expression of chitinase (OS03G0679700 and OS06G0726200) was increased by Cd treatment in S315. In addition, OsHSPs (OS05G0460000, OS08G0500700), OsHSFC2A (OS02G0232000), and OsDJA5 (OS03G0787300) were found differentially expressed after Cd treatment in S315, but changed less in S47. In summary, different rice varieties have different processes and intensities in response to Cd stress. The node A might function as the key tissue for blocking Cd upward transport into the panicle via vigorous processes, including of heavy metal transportation, response to stress, and cell wall.
Association between oral health and incidence of pneumonia: a population-based cohort study from Korea
Authors: Son Minkook , Jo Sangyong , Lee Ji Sung , Lee Dong Hyun 
Published: June 2020

AbstractPneumonia is related to oral health of the elderly and intensive care unit patients. However, studies on the relationship between overall oral health and pneumonia in the general population have been limited. The purpose of this study was to investigate the association between oral health and pneumonia using a nationwide population-based Korean cohort database. Data from 122,251 participants who underwent health screening and oral examinations in 2004 or 2005 were analyzed. Cox proportional hazard regression analysis was performed to evaluate the association between oral health and pneumonia. The risk of pneumonia increased significantly in groups with a higher number of dental caries and missing teeth, with respective adjusted hazard ratios (HRs) and 95% confidence interval (CI) of 1.265 (1.086–1.473; p = 0.0025) and 1.218 (1.113–1.332; p < 0.0001), and decreased significantly in frequent tooth brushing and regular professional dental cleaning groups, with respective adjusted HRs and 95% CI of 0.853 (0.786–0.926; p = 0.0001) and 0.920 (0.855–0.990; p = 0.0255). In addition, regardless of age and comorbidities, oral health status and oral hygiene behaviors were associated with pneumonia. The results indicate that improved oral health may reduce the risk of pneumonia in the general population.
Age-different extent of resection for clinical IA non-small cell lung cancer: analysis of nodal metastasis
Authors: Deng Han-Yu , Zhou Jie , Wang Ru-Lan , Jiang Rui , Qiu Xiao-Ming , Zhu Da-Xing , Tang Xiao-Jun , Zhou Qinghua 
Published: June 2020

AbstractWhether age has any impact on the risk of lymph node (LN) metastasis in patients with early-stage non-small cell lung cancer (NSCLC) remains controversial. Therefore, we aimed to objectively compare the risk of LN metastasis between elderly and young patients so as to justify for age-different extent of surgical resection for treating these patients. We retrospectively collected clinical data of patients undergoing lobectomy or segmentectomy with systematic hilar and mediastinal LN dissection for clinical stage IA peripheral NSCLC from January 2015 to December 2018. Both multivariate logistic regression analysis and propensity score-matched (PSM) analysis were applied to compare the risk of LN metastasis between elderly (>65 years old) and young (≤65 years old) patients. We finally included a total of 590 patients for analysis (142 elderly patients and 448 young patients). In the analysis of unmatched cohorts, young patients tended to have higher rates of hilar/intrapulmonary LN (13.4% VS 9.2%) and mediastinal LN metastasis (10.5% VS 6.3%) than elderly patients. In the multivariate analysis, age was found to be an independent predictor of both hilar/intrapulmonary (Odds ratio(OR) = 2.065, 95%confidence interval(CI): 1.049–4.064, P = 0.036) and mediastinal (OR = 2.400, 95%CI: 1.083–5.316, P = 0.031) LN metastasis. Moreover, in the analysis of well-matched cohorts generated by PSM analysis, young patients had significantly higher rates of hilar/intrapulmonary (18.8% VS 9.4%, P = 0.039) and mediastinal LN metastasis (17.1% VS 6.0%, P = 0.008) than elderly patients. Therefore, age remains to be an independent predictor of LN metastasis in early-stage NSCLC and age-different extent of surgical resection may be justified for these patients.
Low molecular weight silicones induce cell death in cultured cells
Authors: Onnekink Carla , Kappel Rita M. , Boelens Wilbert C. , Pruijn Ger J. M. 
Published: June 2020

AbstractWomen with silicone gel-filled breast implants are exposed to organosilicon compounds, in particular methylsiloxanes, as a result of ‘gel bleed’ and implant rupture. Although these silicones were originally considered to be inert, increasing evidence indicates that they can cause serious health problems. Here, we have analyzed the effects of microdroplets of the methylcyclosiloxanes, in particular D4, on the viability of cultured human cells. The exposure of Jurkat suspension and HeLa monolayer cells to D4 resulted in morphological changes of the cells. The analysis of molecular markers for apoptotic and necrotic processes not only demonstrated that caspases were activated and DNA was fragmented in Jurkat cells exposed to D4, but that also the permeability of the plasma membrane was altered. The induction of apoptotic pathways by D4 was substantiated by the inhibition of caspase activation in cells overexpressing Bcl-2. Cleavage of the caspase-3 substrate U1-70K appeared to be dependent on the D4 content and the efficiency of cleavage decreased with increasing size of the methylcyclosiloxanes (D4, D5 and D6). In addition to Jurkat cells, D4-induced U1-70K cleavage was also observed in HeLa cells, but not in HEp-2 cells. Taken together, these results indicate that D4 and, to a lesser extent, D5 can activate cell-death-related pathways in a cell type-specific fashion and suggest that this phenomenon may contribute to the development of Breast Implant Illness.
Convolutional-neural-network-based diagnosis of appendicitis via CT scans in patients with acute abdominal pain presenting in the emergency department
Authors: Park Jin Joo , Kim Kyung Ah , Nam Yoonho , Choi Moon Hyung , Choi Sun Young , Rhie Jeongbae 
Published: June 2020

AbstractAcute appendicitis is one of the most common causes of abdominal emergencies. We investigated the feasibility of a neural-network-based diagnosis algorithm of appendicitis by using computed tomography (CT) for patients with acute abdominal pain visiting the emergency room (ER). A neural-network-based diagnostic algorithm of appendicitis was developed and validated using CT data from three institutions who visited the ER with abdominal pain and underwent abdominopelvic CT. For input data, 3D isotropic cubes including the appendix were manually extracted and labeled as appendicitis or a normal appendix. A 3D convolutional neural network (CNN) was trained to binary classification on the input. For model development and testing, 8-fold cross validation was conducted for internal validation and an ensemble model was used for external validation. Diagnostic performance was excellent in both the internal and external validation with an accuracy larger than 90%. The CNN-based diagnosis algorithm may be feasible in diagnosing acute appendicitis using the CT data of patients visiting the ER with acute abdominal pain.
Investigations on the complex flows induced by dual-swept/dual-ramp wedges in supersonic flows
Authors: Gao X. , Xiang G. X. , Tang W. J. , Jie X. Z. , Huang X. , He J. Y. , Liu S. A. 
Published: June 2020

AbstractThe caret inlet with a dual-swept/dual-ramp configuration has excellent stealth performance and aerodynamic capability. Most previous investigations on this configuration have focused on experiments and numerical simulations but there are relatively few theoretical investigations. In this study, the flow field characteristics of dual-swept/dual-ramp configuration are investigated analytically and numerically. An analytical approach that combines the shock dynamics with a “spatial dimension reduction” was used to analyze the characteristics of the wave structures and state parameters of the flow field. The effects of the sweep angles and inflow Mach number on the flow field characteristics are investigated. The results indicate that the problem of shock/shock interaction in two intersecting wedges of large back-swept angle is a problem of weak shock interaction. Therefore, the theory of weak shock interaction is used to investigate the flow field characteristics, including the uniformity of the flow field and the total pressure recovery performance.
Physiological and ecological characteristics of Periploca sepium Bunge under drought stress on shell sand in the Yellow River Delta of China
Authors: Wang Xiao , Xia Jiang-Bao , Cao Xue-Bin 
Published: June 2020

AbstractThis study investigated the physiological and ecological changes in P. sepium Bunge and elucidated the physiological regulatory mechanisms underlying the adaptation of P. sepium to drought stress in shell sand. Drought stress led to a significant decrease in the net photosynthesis rate (Pn) and respiration rate of leaves and a decrease in low-intensity light-use efficiency (LUE) and light ecological amplitude. An increase in drought stress led to a considerable decrease in the photosynthetic electron transport rate in the P. sepium leaves and a significant increase in the amount of light energy dissipated as heat. In addition, the photosynthesis process suffered from severe photoinhibition. P. sepium plants counteracted the effects of drought stress primarily by increasing their peroxidase (POD) activity and by regulating membrane lipid peroxidation by secreting greater numbers of osmotic adjustment substances (proline (Pro) and soluble sugars (Ss)) and malondialdehyde (MDA). As drought stress increased, both the stem sap flow rate and the cumulative sap flow of P. sepium decreased considerably. P. sepium Bunge adapts to drought stress through interregulatory activity between photosynthesis, water-related physiological activities, and physiological and biochemical processes, and this species exhibits relatively high adaptive plasticity to drought.
Systemic hypertension associated retinal microvascular changes can be detected with optical coherence tomography angiography
Authors: Sun Christopher , Ladores Carlo , Hong Jimmy , Nguyen Duc Quang , Chua Jacqueline , Ting Daniel , Schmetterer Leopold , Wong Tien Yin , Cheng Ching-Yu , Tan Anna C. S. 
Published: June 2020

AbstractA major complication of hypertension is microvascular damage and capillary rarefaction is a known complication of hypertensive end-organ damage which confers a higher risk of systemic disease such as stroke and cardiovascular events. Our aim was to study the effect of hypertension on the retinal microvasculature using non-invasive optical coherence tomography angiography (OCTA). We performed a case-control study of 94 eyes of 94 participants with systemic hypertension and 46 normal control eyes from the Singapore Chinese Eye Study using a standardized protocol to collect data on past medical history of hypertension, including the number and type of hypertensive medications and assessed mean arterial pressure. Retinal vascular parameters were measured in all eyes using OCTA. In the multivariate analysis adjusting for confounders, compared to controls, eyes of hypertensive patients showed a decrease in the macular vessel density at the level of the superficial [OR 0.02; 95% CI, 0 to 0.64; P 0.027] and deep venous plexuses [OR 0.03; 95% CI, 0 to 0.41; P 0.009] and an increase in the deep foveal avascular zone. This shows that hypertension is associated with reduced retinal vessel density and an increased foveal avascular zone, especially in the deep venous plexus, as seen on OCTA and there is a potential role in using OCTA as a clinical tool to monitor hypertensive damage and identifying at risk patients
Sub-picosecond photo-induced displacive phase transition in two-dimensional MoTe2
Authors: Peng Bo , Zhang Hao , Chen Weiwen , Hou Bowen , Qiu Zhi-Jun , Shao Hezhu , Zhu Heyuan , Monserrat Bartomeu , Fu Desheng , Weng Hongming , Soukoulis Costas M. 
Published: June 2020

AbstractPhoto-induced phase transitions (PIPTs) provide an ultrafast, energy-efficient way for precisely manipulating the topological properties of transition-metal ditellurides and can be used to stabilize a topological phase in an otherwise semiconducting material. Using first-principles calculations, we demonstrate that the PIPT in monolayer MoTe2 from the semiconducting 2H phase to the topological 1T′ phase can be triggered purely by electronic excitations that soften multiple lattice vibrational modes. These softenings, driven by a Peierls-like mechanism within the conduction bands, lead to structural symmetry breaking within sub-picosecond timescales, which is shorter than the timescale of a thermally driven phase transition. The transition is predicted to be triggered by photons with energies over 1.96 eV, with an associated excited carrier density of 3.4 × 1014 cm−2, which enables a controllable phase transformation by varying the laser wavelength. Our results provide insight into the underlying physics of the phase transition in 2D transition-metal ditellurides and show an ultrafast phase-transition mechanism for manipulation of the topological properties of 2D systems.
A Tutorial on Saliva's Role in Swallowing With a Focus on Sjögren's Syndrome
Authors: Letawsky Veronica H. , Schreiber Ann-Marie , Skoretz Stacey A. 
Published: June 2020

Purpose
Saliva is integral to swallowing and necessary for oral health. Understanding saliva's origin and properties is important for swallowing assessment and management. Diseases such as Sjögren's syndrome (SS) can affect saliva negatively, often contributing to dysphagia. Our objectives are to (a) highlight saliva's fundamental role in swallowing, (b) provide a bibliometric overview of literature pertaining to SS pathophysiology and effects on saliva, (c) explore implications of salivary changes on swallowing and quality of life in SS and other populations, and (d) provide suggestions for systematic saliva assessment in practice.

Method
This tutorial reviews saliva production, composition, and involvement in swallowing within health and disease. Using rapid review methodology, we outline the effect of SS on saliva and describe SS etiology, diagnosis, and treatment. We discuss formal saliva assessments and a multidisciplinary approach.

Results

Saliva plays a vital role in swallowing, particularly lubrication, bolus formation, and oral health. SS affects the salivary glands altering salivary flow rate and composition. We identified 55 studies (
N
) measuring salivary changes, grouping them according to four strata demarcated by SS classification criteria updates. For some, xerostomia, dysphagia, and reduced life quality result. Formal saliva assessments include the Clinical Oral Dryness Score, Xerostomia Inventory, and Secretion Rating Scale. Multidisciplinary care is optimal for patients with salivary changes.

Conclusion
Understanding salivary changes in disease may enhance understanding of swallowing and inform dysphagia practice. Expanding swallowing assessments with formal saliva evaluations, and patient perspectives thereof, may aid in developing bespoke treatments, ultimately improving outcomes and quality of life.

Supplemental Material

https://doi.org/10.23641/asha.12456449
Synthesis of Cu2ZnSnSe4 thin‐film solar cells from nanoparticles by a non‐vacuum mechanical ball milling and rapid thermal processing
Authors: Liu Ying , Xu Xiaowei , Liu Ke , Liu Huihong 
Published: June 2020

A truly simple, reproducible, and environmentally friendly non‐vacuum synthesis method for synthesising of carbon‐free Cu2ZnSnSe4 (CZTSe) absorber layer has been presented. The stannite CZTSe nanoparticles with an average size of about 20 nm distributions were fabricated by an ambient mechanical milling procedure using Cu2Se, Zn, Sn, elemental selenium powders as raw materials and non‐toxic ethanol as solvent. The compact CZTSe thin films were formed via an annealing treatment under Ar/Se atmosphere using rapid thermal processing. The optical properties of the obtained CZTSe thin films are the absorption coefficient (α) exceeding 104 cm−1 and Eg of 0.91 eV. The electrical properties of the CZTSe thin films indicate p‐type semiconductor behaviour. The photovoltaic properties of this solar cell are power conversion efficiency of 0.18%.