bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development of in‐house genetic screening for pediatric hearing loss
Authors: Doerfer Karl W. , Sander Tara , Konduri Girija G. , Friedland David R. , Kerschner Joseph E. , Runge Christina L. 
Published: May 2020

AbstractObjectivesTo evaluate the efficiency of in‐house genetic testing for mutations causing the most common types of inherited, nonsyndromic, sensorineural hearing loss (SNHL).MethodsRetrospective cohort study of 200 patients at a single, pediatric medical center with suspected or confirmed hearing loss who underwent either send out vs in‐house genetic testing for mutations in GJB2/GJB6, SLC26A4, and MTRNR1. Primary outcome measure was the difference in mean turnaround time for send‐out vs in‐house genetic testing. Additional outcomes included associations between audiometric findings and genetic test results.ResultsOne hundred four send‐out tests were performed between October 2010 and June 2014, and 100 in‐house tests were performed between November 2014 and November 2016. The mean turnaround time for send‐out testing was 53.7 days. The mean turnaround time for in‐house testing was 18.9 days. This difference was statistically significant (P < .001). The largest component of turnaround time was the amount of time elapsed between receipt of specimen in the lab and final test result. These intervals were 47.0 and 18.3 days for send‐out and in‐house tests, respectively. Notably, the longest turnaround time for in‐house testing (43 days) was less than the average turnaround time for send‐out testing. In addition, we identified two simple audiometric parameters (ie, bilateral newborn hearing screen referral and audiometry showing symmetric SNHL) that may increase diagnostic yield of genetic testing.ConclusionsThe development of in‐house genetic testing programs for inherited SNHL can significantly reduce testing turnaround times. Newborn hearing screening and audiometry results can help clinicians identify patients most likely to benefit from genetic testing.Level of EvidenceIV.
Substrate‐Independent, Reversible, and Easy‐Release Ionogel Adhesives with High Bonding Strength
Authors: Zhu Jingjing , Lu Xiaomeng , Zhang Wei , Liu Xiaokong 
Published: May 2020

AbstractIt is highly desirable to develop reversible and easy‐release adhesives with high bonding strength for a broad range of substrates, while the adhesion of low‐surface‐energy materials (e.g., polytetrafluoretyhylene, PTFE) is challenging. Herein, a substrate‐independent ionogel adhesive is developed by blending an ionic liquid with the copolymer bearing charged segments. By regulating the viscoelastic properties of the ionogel, the adhesive and cohesive strength of the ionogel can be well balanced to maximize the bonding strength for different substrates. The as‐developed ionogel exhibits high bonding strength (>0.3 MPa) for PTFE, plastics, metal, wood, and glass, because the variety of functional groups in the ionogel can form various supramolecular interactions with different substrates. The ionogel also exhibits reversible, easy‐release, and reusable properties for multiple times of bonding and on‐demand debonding without leaving obvious residues on the substrates. The ionogel has high potential for practical applications as temporal adhesives with high bonding strength.
Programmed Transformations of Strong Polyvinyl Alcohol/Sodium Alginate Hydrogels via Ionic Crosslink Lithography
Authors: Li Xuefeng , Xu Danni , Wang Hui , Gong Chunjie , Li Haiyan , Huang Yiwan , Long Shijun , Li Dapeng 
Published: May 2020

AbstractA versatile ionic crosslink lithography (ICL) approach is reported to achieve geometry transitions of strong polyvinyl alcohol/sodium alginate (PVA/SA) hydrogels in a controllable and programmable manner. Specifically, localized PVA/SA and PVA/SA/Fe3+ hydrogel domains of significantly different swellabilities (i.e., in‐plane gradient) are created by patterning and selective ionic crosslinking of one single type of PVA/SA hydrogel. A simple two‐step sequential pre‐ and free‐swelling, or each alone, directs the patterned, inhomogeneous hydrogel to transform in various programmable and quasi‐quantitative ways through local bulging and/or global buckling. All types of shape changing are reversible and repeatable due to the reversible nature of ionic coordination in the hydrogel networks. The flexibility and versatility of 3D printing is also demonstrated in creating through‐thickness gradient in PVA and PVA/SA hydrogel assemblies with similar morphing capability. The ICL approach developed in this work may help shed some light on developing strong and shape morphing hydrogels as soft sensors and actuators and for potentially biomimetic transformations. The ICL approach may also be transferable to fabrication of many other types of hydrogel materials for similar applications.
Zwitterionic Polymer‐Grafted Superhydrophilic and Superoleophobic Silk Fabrics for Anti‐Oil Applications
Authors: Cheng Yan , Wang Jilei , Li Minglun , Fu Fanfan , Zhao Yan , Yu Jing 
Published: May 2020

AbstractA highly anti‐oil fabric membrane is synthesized by surface grafting of zwitterionic poly(sulfobetaine methacrylate) (PSBMA) onto the fabric surface. The fabric membrane is first enzymatically modified to create more reactive amine groups on the surface. A surface‐initiated atom transfer radical polymerization (SI‐ATRP) reaction is then performed to modify the fabric membrane surface with a dense PSBMA brush layer. Surface characterization indicates that the brush‐grafted fabric membrane exhibits increased surface roughness and improved superhydrophilicity. The PSBMA‐modified silk fabrics show a very large contact angle for oil droplets in water, and have excellent oil resistance in air and in water–oil mixtures.
Preliminary study on the function of the POLD1 (CDC2) EXON2 c.56G>A mutation
Authors: Liu Jing , Liu Yu , Fu Jingxuan , Liu Chengeng , Yang Tingting , Zhang Xiaomin , Cao Min , Wang Peichang 
Published: May 2020

AbstractBackgroundFanconi anemia (FA) is a rare recessive disease characterized by DNA damage repair deficiency, and DNA polymerase δ (whose catalytic subunit is encoded by POLD1, also known as CDC2) is closely related to DNA damage repair. Our previous study identified a novel POLD1 missense mutation c.56G>A (p. Arg19>His) in FA family members. However, the function of the POLD1 missense mutation is currently unknown. This study aimed to uncover the biological function of the POLD1 missense mutation.MethodsStable cell lines overexpressing wild‐type POLD1 or mutant POLD1 (c.56G>A, p.Arg19His) were constructed by lentivirus infection. Cell growth curve analysis, cell cycle analysis, and a comet assay were used to analyze the function of the POLD1 mutation.ResultsThe growth and proliferative ability of the cells with POLD1 mutation was decreased significantly compared with those of the wild‐type cells (Student's t test, p < .05). The percentage of cells in the G0/G1 phase increased, and the percentage of cells in the S phase decreased significantly when POLD1 was mutated (Student's t test, p < .05). Moreover, the Olive tail moment value of the cells with the POLD1 mutation was significantly higher than that of the cells with wild‐type POLD1 after H2O2 treatment.ConclusionsThe POLD1 mutation inhibited cell proliferation, slowed cell cycle progression, and reduced DNA damage repair.
Impact of Membrane Types and Catalyst Layers Composition on Performance of Polymer Electrolyte Membrane Fuel Cells
Authors: Mohanta Paritosh Kumar , Ripa Masuma Sultana , Regnet Fabian , Jörissen Ludwig 
Published: May 2020

AbstractPerformance of a low temperature polymer electrolyte membrane fuel cell (PEMFC) is highly dependent on the kind of catalysts, catalyst supports, ionomer amount on the catalyst layers (CL), membrane types and operating conditions. In this work, we investigated the influence of membrane types and CL compositions on MEA performance. MEA performance increases under all practically relevant load conditions with reduction of the membrane thickness from 50 to 15 μm, however further decrease in membrane thickness from 15 to 10 μm leads to reduction in cell voltage at high current loads. A thick anode CL is found to be beneficial under wet operating conditions assuming more pore space is provided to accommodate liquid water, whereas under dry operating conditions, an intermediate thickness of the anode CL is beneficial. When studying the impact of catalyst layer thickness, too thin a catalyst layer again shows reduced performance due to increased ohmic resistance ruled out the performance of the MEAs which have identical Pt crystallite sizes on the cathode CLs i. e. the thinnest the cathode CL, the highest the voltage were achieved at a defined current load. Adaptation of the operating conditions is highly anticipated to achieve the highest MEA performance.
Interventions to improve adverse drug reaction reporting: A scoping review
Authors: Khalili Malahat , Mesgarpour Bita , Sharifi Hamid , Daneshvar Dehnavi Samira , Haghdoost Ali Akbar 
Published: May 2020

AbstractPurposeUnderreporting is the major limitation of a voluntary adverse drug reaction (ADR) reporting system. Many studies have assessed the effectiveness of different interventions designed to improve ADR reporting. The aim of this study was to systematically map interventions and strategies to improve ADR reporting among health care professionals.MethodsThe six‐stage methodological framework of Arksey and O'Malley was used to conduct this scoping review. Ovid MEDLINE, EMBASE, All EBM, and Web of Science were systematically searched from 1999 to February 2019, and the reference lists of the included papers were also searched for gray literature to identify any interventions and strategies that aimed to increase ADR reporting. Two reviewers screened the papers for eligibility based on the inclusion criteria and extracted their key data and analyzed them descriptively.ResultsNinety out of 14 501 papers met the selection criteria. Using computerized registration and active surveillance can increase ADR reporting significantly. Educational interventions performed individually or combined with sending reminders and/or feedback, awards, and providing easier reporting channels can improve ADR reporting over a short to medium term. Multiple interventions may have more impact than single‐component interventions.ConclusionMultiple interventions could cause a greater increase in ADR reporting rates than single interventions. Although educational interventions appear to be effective, few studies have reviewed their long‐term effects to ascertain whether the improvements are sustained over time. Studies with a better methodological quality are required on this subject.
Regulating the pore structure of 2,2,6,6‐tetramethylpiperidine‐1‐oxyl (TEMPO) oxidized cellulose membranes: impact of drying method and organic solvent processing
Authors: Liu Wangyu , Xie Weigui , Wu Lin , Yan Changyuan , Tang Aimin , Luo Yuanqiang 
Published: May 2020

AbstractCellulose‐based membrane is a satisfactory candidate for the separator of lithium‐ion batteries due to its renewability, abundant pore structure and outstanding thermal–chemical stability. In this study, 2,2,6,6‐tetramethylpiperidine‐1‐oxyl (TEMPO) oxidized cellulose fiber (TOCF) membranes with different oxidation degrees were prepared. Membranes with high oxidation degree are faced with a pore closure issue, resulting in low porosity. In order to improve the pore structure, the TOCF membranes were dried differently through air drying, vacuum drying and freeze drying. Furthermore, air‐dried membranes were processed by three organic solvents – n‐butyl alcohol, carbon tetrachloride and n‐heptane. The physical properties, pore structure characteristics, mechanical properties and the electrochemical performance of the membranes were measured and characterized. From the results, freeze drying is found to provide the highest porosity and mean pore diameter. Unfortunately, Young’s modulus of the freeze‐dried membranes is the smallest as well. However, membranes processed by n‐butyl alcohol have weak tensile properties. Compared to non‐processed membranes, membranes processed by organic solvents present better pore structure and significantly better electrochemical performance. With all properties considered, TOCF membranes processed by carbon tetrachloride or n‐heptane are qualified for serving as battery separators as they possess improved pore structure, enhanced Young’s modulus, considerable tensile strength and improved electrochemical properties. © 2020 Society of Chemical Industry
Potential effects of curcumin in the treatment of COVID‐19 infection
Authors: Zahedipour Fatemeh , Hosseini Seyede Atefe , Sathyapalan Thozhukat , Majeed Muhammed , Jamialahmadi Tannaz , Al‐Rasadi Khalid , Banach Maciej , Sahebkar Amirhossein 
Published: May 2020

AbstractCoronavirus disease 2019 (COVID‐19) outbreak is an ongoing pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) with considerable mortality worldwide. The main clinical manifestation of COVID‐19 is the presence of respiratory symptoms, but some patients develop severe cardiovascular and renal complications. There is an urgency to understand the mechanism by which this virus causes complications so as to develop treatment options. Curcumin, a natural polyphenolic compound, could be a potential treatment option for patients with coronavirus disease. In this study, we review some of the potential effects of curcumin such as inhibiting the entry of virus to the cell, inhibiting encapsulation of the virus and viral protease, as well as modulating various cellular signaling pathways. This review provides a basis for further research and development of clinical applications of curcumin for the treatment of newly emerged SARS‐CoV‐2.
First investigation and absolute calibration of clumped isotopes in N2O by mid‐infrared laser spectroscopy
Authors: Kantnerová Kristýna , Yu Longfei , Zindel Daniel , Zahniser Mark S. , Nelson David D. , Tuzson Béla , Nakagawa Mayuko , Toyoda Sakae , Yoshida Naohiro , Emmenegger Lukas , Bernasconi Stefano M. , Mohn Joachim 
Published: May 2020

RationaleUnravelling the biogeochemical cycle of the potent greenhouse gas nitrous oxide (N2O) is an underdetermined problem in environmental sciences due to the multiple source and sink processes involved, which complicate mitigation of its emissions. Measuring the doubly isotopically substituted molecules (isotopocules) of N2O can add new opportunities to fingerprint and constrain its cycle.MethodsWe present a laser spectroscopic technique to selectively and simultaneously measure the eight most abundant isotopocules of N2O, including three doubly substituted species – so called “clumped isotopes”. For the absolute quantification of individual isotopocule abundances, we propose a new calibration scheme that combines thermal equilibration of a working standard gas with a direct mole fraction‐based approach.ResultsThe method is validated for a large range of isotopic composition values by comparison with other established methods (laser spectroscopy using conventional isotopic scale and isotope ratio mass spectrometry). Direct intercomparison with recently developed ultrahigh‐resolution mass spectrometry shows clearly the advantages of the new laser technique, especially with respect to site specificity of isotopic substitution in the N2O molecule.ConclusionsOur study represents a new methodological basis for the measurements of both singly substituted and clumped N2O isotopes. It has a high potential to stimulate future research in the N2O community by establishing a new class of reservoir‐insensitive tracers and molecular‐scale insights.
Optimization Removal of the Ceftriaxone Drug from Aqueous Media with Novel Zero‐Valent Iron Supported on Doped Strontium Hexaferrite Nanoparticles by Response Surface Methodology
Authors: Amiri Shadan , Reza Sohrabi Mahmoud , Motiee Fereshteh 
Published: May 2020

AbstractThe present study, a novel and efficient adsorbent nano zero‐valent iron stabilized on strontium hexaferrite (nZVI/SrFe12O19) was synthesized for removal of ceftriaxone antibiotic from aqueous solution. Its characteristics were analyzed by Fourier transform infrared spectroscopy (FTIR), X‐ray diffraction (XRD), scanning electron microscopy (SEM), and energy‐dispersive X‐ray spectroscopy (EDX). Response Surface Methodology (RSM) based on Box‐Behnken Design (BBD) were used to achieve the optimal experimental conditions. The effects of parameters such as pH (5‐9), initial concentration of ceftriaxone (5–15 mg L−1), and adsorbent dosage (0.05–0.15 g L−1) on removal efficiency were investigated. A mathematical model was studied to predict the removal performance. The significance and adequacy of the model were surveyed using analysis of variance (ANOVA). The results showed that second‐order polynomial model is suitable for the prediction removal of ceftriaxone. The highest removal value of mentioned drug were obtained 98 % at adsorbent dosage of 0.15 g L−1, pH=5, and the pollutant concentration of 10 mg L− 1. Finally, the isotherms and kinetic studies were evaluated. The Langmuir with R2=0.9981 and pseudo‐second‐order with R2=0.9996 were the best isotherm and kinetic, respectively. The thermodynamic parameters such as ΔH0, ΔS0, and ΔG0 were calculated and it was indicated that adsorption process was exothermic and spontaneous.
Photocatalytic Activity of Bi2O3 Enhanced by the Addition of Ce3+/Ce4+ Synthesized by Ethylene Glycol‐assisted Solvothermal Method
Authors: Li Ting , Quan Shanyu , Shi Xuefeng , Liu Cong , Yang Linmei 
Published: May 2020

AbstractThe photocatalysts Bi2O3:Ce3+/Ce4+ (cerium content 0.2, 0.5, 0.8, and 1.1 wt. %) were prepared via one‐pot ethylene glycol‐assisted solvothermal method. The prepared x Ce‐Bi2O3 (x=0.5, 0.8, 1.1) samples present δ‐Bi2O3 phase with space group of Fm‐3 m at room temperature, which indicates that the high‐temperature cubic phase could be stabilized via Ce doping in Bi2O3 lattices. Moreover, the addition of Ce3+/Ce4+ could change the amount of oxygen vacancies (OVs) and adsorbed oxygen on the surface of photocatalysts. The existence of oxygen vacancies and adsorbed oxygen facilitated the generation of superoxide radicals () and hydroxide radicals (), which were the dominant reactive oxygen species for phenol degradation. The photocatalyst containing 0.8 wt. % cerium presented the highest photoactivity than the other synthesized samples. A photodegradation mechanism is proposed based on scavenger and electron spin resonance studies.
Improving the Quality and Luminescence Performance of All‐Inorganic Perovskite Nanomaterials for Light‐Emitting Devices by Surface Engineering
Authors: Shen Zhaohui , Zhao Suling , Song Dandan , Xu Zheng , Qiao Bo , Song Pengjie , Bai Qiongyu , Cao Jingyue , Zhang Gaoqian , Swelm Wageh 
Published: May 2020

AbstractLead halide perovskites and their applications in the optoelectronic field have garnered intensive interest over the years. Inorganic perovskites (IHP), though a novel class of material, are considered as one of the most promising optoelectronic materials. These materials are widely used in detectors, solar cells, and other devices, owing to their excellent charge‐transport properties, high defect tolerance, composition‐ and size‐dependent luminescence, narrow emission, and high photoluminescence quantum yield. In recent years, numerous encouraging achievements have been realized, especially in the research of CsPbX3 (X = Cl, Br, I) nanocrystals (NCs) and surface engineering. Therefore, it is necessary to summarize the principles and effects of these surface engineering optimization methods. It is also important to scientifically guide the applications and promote the development of perovskites more efficiently. Herein, the principles of surface ligands are reviewed, and various surface treatment methods used in CsPbX3 NCs as well as quantum‐dot light‐emitting diodes are presented. Finally, a brief outlook on CsPbX3 NC surface engineering is offered, illustrating the present challenges and the direction in which future investigations are intended to obtain high‐quality CsPbX3 NCs that can be utilized in more applications.
Toward Diffusion Measurements of Colloidal Nanoparticles in Biological Environments by Nuclear Magnetic Resonance
Authors: Padro Daniel , Cienskowski Philipp , Lopez‐Fernandez Sonia , Chakraborty Indranath , Carrillo‐Carrion Carolina , Feliu Neus , Parak Wolfgang J. , Carril Monica 
Published: May 2020

AbstractProtein corona formation on the surface of nanoparticles (NPs) is observed in situ by measuring diffusion coefficients of the NPs under the presence of proteins with a 19F nuclear magnetic resonance (NMR) based methodology. Formation of a protein corona reduces the diffusion coefficient of the NPs, based on an increase in their effective hydrodynamic radii. With this methodology it is demonstrated that the apparent dissociation constant of protein–NP complexes may vary over at least nine orders of magnitude for different types of proteins, in line with the Vroman effect. Using this methodology, the interaction between one type of protein and one type of nanoparticle can be studied quantitatively. Due to the NMR‐based detection, this methodology has no interference by absorption/scattering effects, by which optical detection schemes are affected. By using the potential of the NMR chemical shift, the detection of multiple 19F signals simultaneously opens the possibility to study the diffusion of several NPs at the same time. The 19F labeling of the NPs has negligible effect on their acute toxicity and moderate effect on NPs uptake by cells.
Gene regulation could be attributed to TCF3 and other key transcription factors in the muscle of pubertal heifers
Authors: Lau Li Yieng , Nguyen Loan T. , Reverter Antonio , Moore Stephen S. , Lynn Aaron , McBride‐Kelly Liam , Phillips‐Rose Louis , Plath Mackenzie , Macfarlane Rhys , Vasudivan Vanisha , Morton Lachlan , Ardley Ryan , Ye Yunan , Fortes Marina R. S. 
Published: May 2020

AbstractPuberty is a whole‐body event, driven by the hypothalamic integration of peripheral signals such as leptin or IGF‐1. In the process of puberty, reproductive development is simultaneous to growth, including muscle growth. To enhance our understanding of muscle function related to puberty, we performed transcriptome analyses of muscle samples from six pre‐ and six post‐pubertal Brahman heifers (Bos indicus). Our aims were to perform differential expression analyses and co‐expression analyses to derive a regulatory gene network associate with puberty. As a result, we identified 431 differentially expressed (DEx) transcripts (genes and non‐coding RNAs) when comparing pre‐ to post‐pubertal average gene expression. The DEx transcripts were compared with all expressed transcripts in our samples (over 14,000 transcripts) for functional enrichment analyses. The DEx transcripts were associated with “extracellular region,” “inflammatory response” and “hormone activity” (adjusted p < .05). Inflammatory response for muscle regeneration is a necessary aspect of muscle growth, which is accelerated during puberty. The term “hormone activity” may signal genes that respond to progesterone signalling in the muscle, as the presence of this hormone is an important difference between pre‐ and post‐pubertal heifers in our experimental design. The DEx transcript with the highest average expression difference was a mitochondrial gene, ENSBTAG00000043574 that might be another important link between energy metabolism and puberty. In the derived co‐expression gene network, we identified six hub genes: CDC5L, MYC, TCF3, RUNX2, ATF2 and CREB1. In the same network, 48 key regulators of DEx transcripts were identified, using a regulatory impact factor metric. The hub gene TCF3 was also a key regulator. The majority of the key regulators (22 genes) are members of the zinc finger family, which has been implicated in bovine puberty in other tissues. In conclusion, we described how puberty may affect muscle gene expression in cattle.