bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of Dietary Rumen Undegraded Protein (RUP) Level on Nutrient Intake and Digestion of Lactating Dairy Cows
Authors: Astuti Andriyani , Rochijan Rochijan , Widyobroto Budi Prasetyo 
Published: December 2020

The objective of this study was to examine the effect of different levels of rumen undegraded protein (RUP) in diets of lactating dairy cows on feed intake and nutrient digestion. The experiment was run according to completely randomized design with three treatments of complete feed rations containing different levels of rumen undegraded protein (RUP) and four replications for each treatment. In a digestibility trial, twelve Friesian Holstein cows were divided into three groups at each group consist of four cows and given three dietary treatments containing three levels of RUP, namely 7.43% of total crude protein /CP (P0), 8.49% of total CP (P1), and 9.45% of total CP (P2). Diets consisted of forages (King grass, Pennisetum hybrid) and concentrates at ratio of 50:50 in dry matter basis. The diets contained 18.25% CP and 61.75% total digestible nutrient (TDN). The cows fed diet (P0) were considered the control group. Drinking water was given ad libitum. This experiment was conducted for 21 days of adaptation period and seven days of collection period. In the collection period, samples of feed, refusal feed, and faecal were collected to get the chemical content includes dry matter (DM), organic matter (OM), ether extract (EE), crude fiber (CF), crude protein (CP), nitrogen-free extract (NFE) and total digestible nutrients (TDN). The data obtained were processed with one-way ANOVA (analysis of variance) and the difference existed between the means (P<0.05) would be analyzed with Duncan’s new multiple range test. The results showed that cows fed diet P2 (9.45% RUP in CP) in had significantly higher feed and nutrient intake (DM, OM, CP, CF, EE, TDN) and higher DM and OM digestibility compared to the other two treatments.
Use of an Omental Flow-Through Flap for Recipient Vessels in the Reconstruction of a Lumbar Spine Defect
Authors: Hatchell Alexandra C. , Aoude Ahmed , Aldebeyan Sultan , McKenzie C. David , Lewkonia Peter , de Haas Willian 
Published: December 2020

Case:
Spine reconstruction after en bloc spondylectomy is challenging and may require multidisciplinary intervention. En bloc spine tumor resection with embolization of local recipient vessels for tumor control limits reconstructive options. Herein, we describe a case where combined efforts from orthopaedic, general, and plastic surgery teams permitted the successful reconstruction of a multilevel lumbar vertebral defect.

Conclusion:
A fibula-free flap within a titanium cage construct anastomosed to the left gastroepiploic vessels via a pedicled omental flow-through flap is a viable and novel method for reconstruction of a multilevel vertebral defect.
Sialic acid-binding lectin-modified fructose-coated nanoparticles: a promising targeted therapeutic synthetic for breast cancers
Authors: He Lin , Zhang Biyuan , Song Yuhua , Wang Haiji 
Published: December 2020

Abstract
Background: Sialic acid-binding lectin (cSBL) specifically kills tumor cells rather than healthy cells. Glycopolymer-coated nanoparticles are selectively ingested by tumor cells because they can interact with the enriched carbohydrate receptors located on the surface of tumor cells. In this context, we synthesized cSBL-modified fructose-coated nanoparticles (LMFN) and cSBL-modified glucose-coated nanoparticles (LMGN) to investigate their anticancer activity in various molecular subtypes of breast cancer cell lines.Methods: The syntheses of fructose-coated nanoparticles and glucose-coated nanoparticles were based on the chemicals of 1,2:4,5-di-O-isopropylidene-β-d-fructopyranose and 1,2:4,5-di-O-isopropylidene-β-d-glucopyranose, respectively. The carbodiimide-based method was employed to synthesize LMFN and LMGN. The antitumor mechanism was explored by cell cycle analysis with flowcytometry and the antitumor activity was assessed by cytotoxicity assay and multiple drug effects analysis.Results: The cytotoxicity assay showed that LMFN had robust antitumor activity against all breast cancer phenotype cell lines whereas LMGN was rarely efficacious to against human epidermal growth factor receptor 2-positive/overexpression (HER2+/overexpression) breast cancer cells. The intrinsic reason for these findings was that the overexpression of fructose transporter, GLUT5, was observed in all breast cancer subtype cell lines but only a paucity of glucose transporter, GLUT1, was expressed in HER2+/overexpression breast cancer cell lines that dampened the uptake of LMGN to these cells. The cell cycle analysis indicated that the anticancer activity of LMFN was achieved by arresting cell cycle in S phase. The multiple drug effects analysis suggested the synergistic effect in the combinations of LMFN and tamoxifen to kill estrogen receptor+ breast cancer cells and LMFN and trastuzumab to kill HER2+/overexpressed breast cancer cells.Conclusion: Our work pinpoints that LMFN may be a new-onset selection for molecularly targeted therapy of breast cancers and paves the way for establishing its clinical application in the future.
Identifying Periampullary Regions in MRI Images Using Deep Learning
Authors: Tang Yong , Chen Xinpei , Wang Weijia , Wu Jiali , Zheng Yingjun , Guo Qingxi , Shu Jian , Su Song 
Published: December 2020

Abstract
Background: Development and validation of a deep learning method to automatically segment the peri-ampullary (PA) region in magnetic resonance imaging (MRI) images. Methods: A group of patients with or without periampullary carcinoma (PAC) was included. The PA regions were manually annotated in MRI images by experts. Patients were randomly divided into one training set and one validation set. A deep learning method to automatically segment the PA region in MRI images was developed using the training set. The segmentation performance of the method was evaluated in the validation set. Results: The deep learning algorithm achieved optimal accuracies in the segmentation of the PA regions in both T1 and T2 MRI images. The value of the intersection over union (IoU) was 0.67 and 0.68 for T1 and T2 images, respectively. Conclusions: Deep learning algorithm is promising with accuracies of concordance with manual human assessment in segmentation of the PA region in MRI images. This automated non-invasive method helps clinicians to identify and locate the PA region using preoperative MRI scanning.
The Risk of Lymph Node Metastasis in Early Gastric Cancer Conforming to Indications of Endoscopic Resection and Pylorus-Preserving Gastrectomy: A Single-Center Retrospective Study
Authors: Wu YanZhang , Wang Zhixiong , Li Guanghua , Wang Zhao , Zhou ZhiHao 
Published: December 2020

Abstract
Background: Lymph node metastasis (LNM) status is an important prognostic factor that strongly influences the treatment decision of early gastric cancer (EGC). This study aimed to evaluate the pattern and clinical significance of LNM in EGC.Methods: Patients with EGC who underwent radical gastrectomy were enrolled. Their clinicopathological features, pathological reports, and prognostic data were collected and analyzed.Results: Three hundred fifty-four patients with EGC were enrolled. The incidence of LNM in patients with EGC was 18.36% (65/354). The rates of D1 and D2 station metastases were 12.1% (43/354) and 6.214% (22/354), respectively. The rates of LNM in absolute indication of endoscopic resection and expanded indication were 3.27% (2/61) and 28.55% (4/14), respectively. Skip LNM was observed in 3.67% (13/354) of patients. For those with middle-third EGC, the metastasis rate of the No. 5 lymph node was 3.05% (5/164). The independent risk factors for LNM were tumors measuring >30 mm, poorly differentiated tumors, and lymphovascular invasion (all P < 0.05，area under the curve = 0.783). Five-year disease-free survival rates of patients with EGC with and without LNM were 96.26% and 79.17%, respectively (P = 0.011). Tumors measuring >20 mm and LNM were independent predictive factors for poor survival outcome in patients with EGC.Conclusions: Patients with EGC conforming to expanded indications have a relatively high risk of LNM and may not be suitable for endoscopic submucosal dissection. Pylorus-preserving gastrectomy for patients with middle-third EGC remains controversial due to the high metastasis rate of the No. 5 lymph node.
Effect of the Timing of Frozen Embryo Transfer on Pregnancy Outcomes in High Ovarian Response Patients Undergoing Freeze-All Strategy
Authors: Zuo Na , Gao Yingzhuo , Zhang Ningning , Li Da , Wang Xiuxia 
Published: December 2020

Abstract
Background: Frozen embryo transfer (FET) can greatly improve the pregnancy outcomes for high ovarian response (HOR) population. However, it is not known whether the impaired endometrial receptivity derived from controlled ovarian hyperstimulation (COH) can be fully recovered in the first menstrual cycle after oocyte retrieval, and whether the timing of FET is a risk factor on pregnancy outcomes in HOR population undergoing freeze-all strategy.Methods: A retrospective cohort study to compare the pregnancy outcomes of the immediate and delayed FET groups in HOR population undergoing freeze-all strategy. Propensity score matching was used to make the potential risk factors of the immediate and delayed FET groups comparable. Multivariable regression analysis was used to study the effect of the timing of FET on pregnancy outcomes in the entire cohort and propensity score-matched cohort, even in different COH protocol cohorts as subgroup analysis.Results: We showed that the immediate FET group were no worse than delayed FET group in the entire cohort [clinical pregnancy rate (CPR), adjusted odd ratio (OR), 0.942, 95% confidence interval (CI), 0.784-1.133; spontaneous abortion rate (SAR), adjusted OR, 1.118, 95% Cl (0.771-1.623); live birth rate (LBR), adjusted OR, 1.060, 95% Cl (0.886-1.267)]. The same results were obtained by χ2 test in the propensity score-matched cohort (CPR, 60.5% versus 63.5%; SAR, 11.6% versus 12.3%; LBR, 48% versus 49.3%) (P > 0.05). Subgroup analysis indicated that pregnancy outcomes of immediate FET were non-inferior to delayed FET in short-acting gonadotropin-releasing hormone agonist (GnRH-a) long protocol (P > 0.05). The SAR of the immediate FET group were lower than that of the delayed FET group in GnRH antagonist protocol (adjusted OR, 0.646, 95% CI, 0.432-0.966) and long-acting GnRH-a long protocol (adjusted OR, 0.375, 95% CI, 0.142-0.990) (P < 0.05), no differences were observed in CPR and LBR (P > 0.05).Conclusions: These findings indicate that immediate FET might not affect pregnancy outcomes in HOR patients undergoing freeze-all strategy. Delaying FET could increase the SAR in GnRH-ant and long-acting GnRH-a long protocols.
Factors Associated With Gestational Diabetes Mellitus in Rural Areas of Limpopo Province: A Descriptive Analysis
Authors: Ntshauba Elelwani Thelma , Maimela Eric , Ntuli Thembelihle Sam 
Published: December 2020

Abstract
Background: Sustainable Developmental Goal (SDG) no 3 of the United Nations Organisation places emphasise on ensuring healthy lives and promoting the well-being of all people of all age groups. Yet the prevalence of Gestational Diabetes Mellitus (GDM) is increasing and this can have an adverse impact on maternal and infant health and well-being. Currently, more studies have been conducted in International countries on prevalence and risk factors of GDM and few in South African Context. The current study sought to investigate the prevalence, risk factors, maternal and infant outcomes of women with gestational diabetes mellitus in Mopani District. Methods: A cross-sectional descriptive study was conducted amongst pregnant women during Antenatal Care Clinic Visits at Nkhensani Hospital, Nkhensani Gateway clinic and Giyani Healthcare Centre. Information from participants was collected using adapted questionnaire from Michigan Diabetes Research and Training Centre DCP 2.0 and data entry form, captured on Microsoft excel spread sheet and analysed using Statistical Package for Social Sciences (SPSS) and p-value of <0.05 was considered statistically significant.Results: One hundred and one (101) pregnant women (74%) who were attending antenatal care clinic visits at Nkhensani Hospital, Nkhensani Gateway Clinic and Giyani Healthcare Centre completed the questionnaire. Prevalence of Gestational Diabetes Mellitus (GDM) in Mopani District was 1.9% (2 women). Pregnant women above 30 years with secondary education, employed, obese and at gestational age of 31-35 weeks were more likely to present with GDM. A family history of diabetes was significantly associated with development of GDM. Conclusions: The use of various GDM screening strategies across population and countries resulted in discrepancies in the prevalence rate. Universal GDM screening strategy which will benefit our socioeconomic and clinical context to ensure that the true burden of GDM is determined needs to be adopted.
A Deep Learning Based Method for Forecasting Gold Price with Respect to Pandemics
Authors: Khani Mahtab Mohtasham , Vahidnia Sahand , Abbasi Alireza 
Published: December 2020

Abstract
The spread of COVID-19 in the world had a devastating impact on the world economy, trade relations, and globalization. As the pandemic advances and new potential pandemics are on the horizon, a precise analysis of recent fluctuations of trade becomes necessary for international decisions and controlling the world in a similar crisis. The COVID-19 pandemic made a new pattern of trade in the world and affected how businesses work and trade with each other. It means that every potential pandemic or any unprecedented event in the world can change the market rules. This research develops a novel model to have a proper estimation of the stock market values with respect to the COVID-19 dataset using long short-term memory networks (LSTM).The nature of the features in each pandemic is totally different, thus, prediction results for a pandemic by a specific model cannot be applied to other pandemics. Hence, recognizing and extracting the features which affect the pandemic is the highest priority. In this study, we develop a framework, providing a better understanding of the features and feature selection. This study is based on a preliminary analysis of such features for enhancing forecasting models' performance against fluctuations in the market.Our forecasts are based on the market value data and COVID-19 pandemic daily time-series data (i.e. the number of new cases). In this study, we selected Gold price as a base for our forecasting task which can be replaced by any other markets. We have applied Convolutional Neural Networks (CNN) LSTM, Vector Output Sequence LSTM, Bidirectional LSTM, and Encoder-Decoder LSTM on our dataset, and our results achieved an MSE of 6.0e-4, 8.0e-4, and 2.0e-3 on the validation set respectfully for one day, two days, and 30 days predictions in advance which are outperforming other proposed method in the literature.
Is Oral Health All About Tooth Brushing? A Longitudinal Study in Individuals With Brain Injury in A Neurorehabilitation Setting
Authors: Kothari Simple , Nascimento Gustavo , Jakobsen MIlle , Nielsen Jørgen , Kothari Mohit 
Published: December 2020

Abstract
Objective: To investigate oral health changes and its associated factors during hospitalization in individuals with acquired brain injury (ABI).Material and Methods: Sixty-one individuals were recruited to evaluate the acute changes in oral health by performing plaque, calculus, bleeding on probing (BOP) and bedside oral examination (BOE) at week 1 and week 5. Individuals’ brushing habits, eating difficulties, onset of pneumonia etc. were retrieved from e-journal. Association between oral-health outcomes to systemic variables were investigated through multilevel regression models.Results: Dental plaque (P=0.01) and total BOE score (P<0.05) decreased over time but not the calculus (P=0.30), BOP (P=0.06) and increase in tooth brushing frequency (P=0.06). Reduction in plaque, and BOE over time were negatively associated with higher score of periodontitis at baseline (coef. -6.8; -1.0, respectively), which in turn were associated with an increased proportion of BOP (coef. ≈ 15.0). Increased proportion of calculus was associated with eating difficulties (coef. 2.3) and onset of pneumonia (coef. 6.2).Conclusions: Nursing care has been fundamental in improving oral health but non-significant improvement in calculus, BOP and brushing frequency indicates a need for development in existing oral care program through academic-clinical partnership keeping eating difficulties and patients’ vegetative and cognitive state in consideration.
Adverse Events in Thyroid Surgery. Observational Study in Three Surgical Units With High Volume/Year.
Authors: Rio Paolo Del , Carcoforo Paolo , Medas Fabio , Bonati Elena , Loderer Tommaso , Radica Margherita Koleva , Calò Piergiorgio 
Published: December 2020

Abstract
Background Thyroid surgery, performed for benign or malignant pathologies, is one of the most frequently performed procedures and its frequency has even been increasing in recent years. Postoperative bleeding, recurrent laryngeal nerve (RLN) palsy, associated to dysphonia, dysphagia, dyspnea, and hypoparathyroidism represent the most fearful and common complications. We conducted a multicenter, observational study of retrospectively collected data in three high-volume referral centers, enrolling all patients undergone to thyroid surgery between January 2016 and December 2017 in Parma University Hospital, Cagliari University Hospital and Ferrara University Hospital.Materials Patients were divided into 5 groups, differentiated thyroid carcinoma, medullary thyroid carcinoma, non-toxic benign pathology, hyperfunctioning benign pathology and NIFTP (Non-invasive Follicular Thyroid neoplasm with Papillary-like nuclear features). A follow up at 7 and 30 days was executed, evaluating the onset of paresthesia, dysphonia and dysphagia. A six-month follow-up was conducted in cases of early complications.Results Totally, 1252 patients were eligible for the study: 907 female and 345 male, with a female to male ratio of 2.6:1 and an average age of 53.428. Total thyroidectomy was performed in 1022 cases, lobectomy in 230.After 6 months we recorded paresthesia in 0.5%, dysphonia in 1.8% and dysphagia in 0.5%.Conclusion Our study confirms once again that a share of morbidity escapes the possibilities of prediction and control by the operator, depending on patient anamnestic, pathological or anatomical factors.
WITHDRAWN: Electrocatalytic glycerol oxidation with concurrent hydrogen evolution utilizing an efficient MoOx/Pt catalyst
Authors: Johnsson Mats , Yu Xiaowen , Santos Egon Campos dos , White Jai , Salazar-Alvarez German , Pettersson Lars , Cornell Ann 
Published: December 2020

Abstract
Glycerol electrolysis affords a green and energetically favorable route for the production of value-added chemicals at the anode and H2 production in parallel at the cathode. Here, we report a novel and facile method for trapping Pt nanoparticles at oxygen vacancies of molybdenum oxide (MoOx) nanosheets, yielding a high-performance MoOx/Pt composite electrocatalyst for both the glycerol oxidation reaction (GOR) and the hydrogen evolution reaction (HER) in alkaline electrolytes. Combined electrochemical experiments and theoretical calculations revealed the important role of MoOx nanosheets for the adsorption of glycerol molecules in GOR and the dissociation of water molecules in HER, as well as the strong electronic interaction with Pt. With MoOx/Pt electrodes serving as both cathode and anode, we achieve two-electrode glycerol electrolysis at a cell voltage of 0.70 V to reach a current density of 10 mA cm− 2, which is 0.90 V less than that required for water electrolysis.
Full-Quantitative Analysis of Cementless Stem Hammering Sound Changes During Total Hip Arthroplasty.
Authors: Zhuang Xu , Homma Yasuhiro , Ishii Seiya , Morohashi Itaru , Iwase Hideaki , Baba Tomonori , Tanabe Hiroki , Banno Sammy , Watari Taiji , Sato Taichi , Kaneko Kazuo , Ishijima Muneaki 
Published: December 2020

Abstract
Full-quantitative characterization has not been performed to analyze changes in the hammering sound in cementless hip arthroplasty. We analyzed the frequency spectrum of the hammering sound during stem insertion for 20 cases of uncomplicated cementless total hip arthroplasty for osteoarthritis using a proximal-coated stem. The absolute sound pressure (Pa) and normalized sound pressure of each frequency bands in early and late stage of femoral stem insertion were determined by the Fast Fourier Transform analysis. The absolute sound pressures (Pa) of a majority of frequency bands was significantly higher in the late-stage stem insertion than in the early stage The 1.0–1.5-kHz frequency band showed a significant change in normalized sound pressure in all cases between the early and late stages of stem insertion (p=0.000). The femoral morphology and canal fill ratio were correlated with late stage normalized sound pressure in specific frequency bands. In the 5.0–5.5 kHz band, the Dorr A femoral morphology was significantly higher normalized sound pressure than those in the Dorr B (p=0.004). This study revealed the hammering sound frequency with full-quantitative value altered during cementless stem insertion. Frequency bands of 1.0–1.5 kHz, 5.0–5.5 kHz were the key bands for predicting stem fixation.
Mycoremediation of an Agricultural Salty Soil Contaminated With Endosulfan by Penicillium Crustosum&nbsp;
Authors: Landa Anbu , Vázquez Refugio Rodríguez , Carillo Teresa Guadalupe Roldán 
Published: December 2020

Abstract
This is the first report about the application of Penicillium crustosum isolated from Citrus sinensis peel for endosulfan biodegradation and improvement of soil characteristics of a salty agricultural soil contamined with endosulfan; getting a degradation of 93 ± 4.7%, after one month of treatment. Metabolites production was also evaluated, finding that sulphate endosulfan and mono alcohol endosulfan were the main compounds produced for that reason an oxidative pathway is suggest. Degradation was enhanced by the biological surface active agent produced by the fungus during soil mycoremediation, where a DST up to 17 ± 0.58 mN m-1, was determined, being the first time that the in situ production for this kind of application was studies. Additionally, an improvement in soil quality (reduction of clay and salinity, and an increase of soluble phosphorus, carbon content and organic matter) was observed during the mycoremediation process. The phytotoxicity of the pesticide on Phaseolus leptostachyus crop was evaluated; in the soil without the fungus the pesticide translocated into the crop, presenting a negative effect in germination index, root length and weight, aerial weight and proline content. This contrasted with the crop develop in the soil treated with P. crustosum, which had better agronomic characteristics. In general, was possible appreciated that the addition of P. crustosum, present a lot of benefits for agricultural applications.