bluuebunny/crossref_metadata_2025_split huggingface dataset:
Iodine status of consumers of milk-alternative drinks v. cows’ milk: data from the UK National Diet and Nutrition Survey
Authors: Dineva M. , Rayman M. P. , Bath S. C. 
Published: September 2020

AbstractMilk is the main source of iodine in the UK; however, the consumption and popularity of plant-based milk-alternative drinks are increasing. Consumers may be at risk of iodine deficiency as, unless fortified, milk alternatives have a low iodine concentration. We therefore aimed to compare the iodine intake and status of milk-alternative consumers with that of cows’ milk consumers. We used data from the UK National Diet and Nutrition Survey from years 7 to 9 (2014–2017; before a few manufacturers fortified their milk-alternative drinks with iodine). Data from 4-d food diaries were used to identify consumers of milk-alternative drinks and cows’ milk, along with the estimation of their iodine intake (µg/d) (available for n 3976 adults and children ≥1·5 years). Iodine status was based on urinary iodine concentration (UIC, µg/l) from spot-urine samples (available for n 2845 adults and children ≥4 years). Milk-alternative drinks were consumed by 4·6 % (n 185; n 88 consumed these drinks exclusively). Iodine intake was significantly lower in exclusive consumers of milk alternatives than cows’ milk consumers (94 v. 129 µg/d; P < 0·001). Exclusive consumers of milk alternatives also had a lower median UIC than cows’ milk consumers (79 v. 132 µg/l; P < 0·001) and were classified as iodine deficient by the WHO criterion (median UIC < 100 µg/l), whereas cows’ milk consumers were iodine sufficient. These data show that consumers of unfortified milk-alternative drinks are at risk of iodine deficiency. As a greater number of people consume milk-alternative drinks, it is important that these products are fortified appropriately to provide a similar iodine content to that of cows’ milk.
Using occupancy-based camera-trap surveys to assess the Critically Endangered primateMacaca nigraacross its range in North Sulawesi, Indonesia
Authors: Johnson Caspian L. , Hilser Harry , Linkie Matthew , Rahasia Rivo , Rovero Francesco , Pusparini Wulan , Hunowu Iwan , Patandung Alfons , Andayani Noviar , Tasirin John , Nistyantara Lukita A. , Bowkett Andrew E. 
Published: September 2020

AbstractPrimates are one of the most threatened groups of mammals. Understanding their patterns of population occurrence and abundance, especially in response to threats, is critical for informing conservation action. The crested black macaqueMacaca nigrais the only Critically Endangered species of Sulawesi's seven endemic macaques. Little is known about its distribution or its response to deforestation and hunting. We conducted a camera-trap survey across the entire species range using an occupancy-based analytical approach to (1) establish the first range-wide baseline of occurrence, (2) investigate how environmental and anthropogenic factors influence occurrence, (3) identify priority conservation subpopulations, and (4) test the efficacy of the sampling and analytical protocol for temporal monitoring ofM. nigrausing occupancy as the state variable. From 9,753 camera-trap days,M. nigrawas detected on 473 days at 77 of the 111 camera locations. Species occupancy was 0.66 and highest inside protected areas and closed canopy forest. We identified eight distinct subpopulations, based on distribution and forest fragment size. To inform future monitoring, we used a power analysis to determine if our effort would allow us to detect inter-annual occupancy declines of 10%, and found that 90 camera locations surveyed for 3 months (8,100 camera days) across three consecutive seasons is the effort required to detect such change with 80% certainty. Our study underscores the importance of well-managed protected areas and intact forests for the long-term survival of the crested black macaque, and tests the effectiveness of camera traps to monitor primates at the landscape scale.
Clinical characteristics of imported and second-generation coronavirus disease 2019 (COVID-19) cases in Shaanxi outside Wuhan, China: a multicentre retrospective study
Authors: Shi Puyu , Ren Guoxia , Yang Jun , Li Zhiqiang , Deng Shujiao , Li Miao , Wang Shasha , Xu Xiaofeng , Chen Fuping , Li Yuanjun , Li Chunyan , Yang Xiaohua , Xie Zhaofeng , Wu Zhengxia , Chen Mingwei 
Published: September 2020

AbstractThe mortality of coronavirus disease 2019 (COVID-19) differs between countries and regions. This study aimed to clarify the clinical characteristics of imported and second-generation cases in Shaanxi. This study included 134 COVID-19 cases in Shaanxi outside Wuhan. Clinical data were compared between severe and non-severe cases. We further profiled the dynamic laboratory findings of some patients. In total, 34.3% of the 134 patients were severe cases, 11.2% had complications. As of 7 March 2020, 91.8% patients were discharged and one patient (0.7%) died. Age, lymphocyte count, C-reactive protein, erythrocyte sedimentation rate, direct bilirubin, lactate dehydrogenase and hydroxybutyrate dehydrogenase showed difference between severe and no-severe cases (allP< 0.05). Baseline lymphocyte count was higher in survived patients than in non-survivor case, and it increased as the condition improved, but declined sharply when death occurred. The interleukin-6 (IL-6) level displayed a downtrend in survivors, but rose very high in the death case. Pulmonary fibrosis was found on later chest computed tomography images in 51.5% of the pneumonia cases. Imported and second-generation cases outside Wuhan had a better prognosis than initial cases in Wuhan. Lymphocyte count and IL-6 level could be used for evaluating prognosis. Pulmonary fibrosis as the sequelae of COVID-19 should be taken into account.
Supporting maternal and child nutrition: views from community members in rural Northern Ghana
Authors: Debpuur Cornelius , Nonterah Engelbert A , Chatio Samuel T , Adoctor James K , Dambayi Edith , Beeri Paula , Nonterah Esmond W , Ayi-Bisah Doreen , Watson Daniella , Kehoe Sarah H , Dalaba Maxwell A , Ofosu Winfred , Aborigo Raymond , Welaga Paul , Oduro Abraham R , Newell Marie-Louise , Barker Mary ,   for the INPreP Study Group
Published: September 2020

AbstractObjective:Despite efforts to improve maternal and child nutrition, undernutrition remains a major public health challenge in Ghana. The current study explored community perceptions of undernutrition and context-specific interventions that could improve maternal and child nutrition in rural Northern Ghana.Design:This exploratory qualitative study used ten focus group discussions to gather primary data. The discussions were recorded, transcribed and coded into themes using Nvivo 12 software to aid thematic analysis.Setting:The study was conducted in rural Kassena-Nankana Districts of Northern Ghana.Study participants:Thirty-three men and fifty-one women aged 18–50 years were randomly selected from the community.Results:Most participants reported poverty, lack of irrigated agricultural land and poor harvests as the main barriers to optimal nutrition. To improve maternal and child nutrition, study participants suggested that the construction of dams at the community level would facilitate all year round farming including rearing of animals. Participants perceived that the provision of agricultural materials such as high yield seedlings, pesticides and fertiliser would help boost agricultural productivity. They also recommended community-based nutrition education by trained health volunteers, focused on types of locally produced foods and appropriate ways to prepare them to help improve maternal and child nutrition.Conclusion:Drawing on these findings and existing literature, we argue that supporting community initiated nutrition interventions such as improved irrigation for dry season farming, provision of agricultural inputs and community education could improve maternal and child nutrition.
Responses of Precipitation and Runoff to Climate Warming and Implications for Future Drought Changes in China
Authors: Gu Lei , Chen Jie , Yin Jiabo , Xu Chong‐Yu , Zhou Jianzhong 
Published: September 2020

AbstractThe Clausius‐Clapeyron relationship holds that the atmospheric water vapor content enhances with warming temperatures, suggesting intensifications of precipitable water and also altering runoff generation. Drought conditions are determined by variations in water fluxes such as precipitation and runoff, which tightly connect with temperature scaling characteristics. However, whether and how water fluxes' scaling with temperatures may affect the evolution of droughts under climate change has not yet been systematically investigated. This study develops a cascade modeling chain consisting of the climate model ensemble, bias correction technique, and hydrological models to investigate the precipitation and runoff scaling relationships with warming temperatures under the current (1961–2005) and future periods (2011–2055 and 2056–2100), as well as their implications on future drought changes across 151 catchments in China. The results show that (1) precipitation (runoff) scaling relationships with temperatures are stable during different time periods; (2) return level analysis indicates drought risks are projected to become (1–10 times) more severe across central and southern catchments, where the precipitation (runoff) strengthens with rising temperatures up to a peak point and then decline in a hotter environment. The northeastern and western catchments, where a monotonic increasing scaling type dominated, are accompanied by drought mitigations for two future periods; (3) future changes in hydrological droughts relative to the baseline are (1–5 times) larger than those in meteorological droughts. These results imply that changes in future drought risks are highly dependent on the present precipitation (runoff)‐temperature relationships, suggesting a meaningful implication of scaling types for future drought prediction.
A multicentre, randomised, open-label, parallel-group Phase 2b study of belotecan versus topotecan for recurrent ovarian cancer
Authors: Kim Hee Seung , Park Sang-Yoon , Park Chan-Yong , Kim Young Tae , Kim Beob-Jong , Song Yong Jung , Kim Byoung-Gie , Kim Yong Beom , Cho Chi-Heum , Kim Jong-Hyeok , Song Yong Sang 
Published: September 2020

Abstract
Background
This Phase 2b study compared the efficacy and toxicity of belotecan and topotecan in recurrent ovarian cancer.

Methods
Patients with platinum-sensitive recurrent or platinum-resistant recurrent ovarian cancer (PRROC) were randomised 1:1 to receive belotecan 0.5 mg/m2 or topotecan 1.5 mg/m2 for five consecutive days every 3 weeks. The primary endpoint was overall response rate (ORR); secondary endpoints were progression-free survival (PFS), overall survival (OS), and toxicity.

Results
A total of 140 (belotecan, n = 71; topotecan, n = 69) and 130 patients (belotecan, n = 66; topotecan, n = 64) were included in the intention-to-treat (ITT) and per-protocol (PP) populations. ORR did not differ significantly between the belotecan and topotecan groups (ITT, 29.6% versus 26.1%; PP, 30.3% versus 25%). Although PFS did not differ between the groups, belotecan was associated with improved OS compared with topotecan in the PP population (39.7 versus 26.6 months; P = 0.034). In particular, belotecan showed longer OS in PRROC and non-high-grade serous carcinoma (non-HGSC; PP, adjusted hazard ratios, 0.499 and 0.187; 95% confidence intervals 0.255–0.977 and 0.039–0.895). Furthermore, there were no differences in toxicities between the two groups.

Conclusions
Belotecan was not inferior to topotecan in terms of overall response for recurrent ovarian cancer.

Clinical trial registration
NCT01630018.
Lysosomal quality control of cell fate: a novel therapeutic target for human diseases
Authors: Zhu Sheng-yu , Yao Ren-qi , Li Yu-xuan , Zhao Peng-yue , Ren Chao , Du Xiao-hui , Yao Yong-ming 
Published: September 2020

AbstractIn eukaryotic cells, lysosomes are digestive centers where biological macromolecules are degraded by phagocytosis and autophagy, thereby maintaining cellular self-renewal capacity and energy supply. Lysosomes also serve as signaling hubs to monitor the intracellular levels of nutrients and energy by acting as platforms for the assembly of multiple signaling pathways, such as mammalian target of rapamycin complex 1 (mTORC1) and adenosine 5′-monophosphate (AMP)-activated protein kinase (AMPK). The structural integrity and functional balance of lysosomes are essential for cell function and viability. In fact, lysosomal damage not only disrupts intracellular clearance but also results in the leakage of multiple contents, which pose great threats to the cell by triggering cell death pathways, including apoptosis, necroptosis, pyroptosis, and ferroptosis. The collapse of lysosomal homeostasis is reportedly critical for the pathogenesis and development of various diseases, such as tumors, neurodegenerative diseases, cardiovascular diseases, and inflammatory diseases. Lysosomal quality control (LQC), comprising lysosomal repair, lysophagy, and lysosomal regeneration, is rapidly initiated in response to lysosomal damage to maintain lysosomal structural integrity and functional homeostasis. LQC may be a novel but pivotal target for disease treatment because of its indispensable role in maintaining intracellular homeostasis and cell fate.
Broad host range of SARS-CoV-2 and the molecular basis for SARS-CoV-2 binding to cat ACE2
Authors: Wu Lili , Chen Qian , Liu Kefang , Wang Jia , Han Pengcheng , Zhang Yanfang , Hu Yu , Meng Yumin , Pan Xiaoqian , Qiao Chengpeng , Tian Siyu , Du Pei , Song Hao , Shi Weifeng , Qi Jianxun , Wang Hong-Wei , Yan Jinghua , Gao George Fu , Wang Qihui 
Published: September 2020

AbstractSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of the recent pandemic COVID-19, is reported to have originated from bats, with its intermediate host unknown to date. Here, we screened 26 animal counterparts of the human ACE2 (hACE2), the receptor for SARS-CoV-2 and SARS-CoV, and found that the ACE2s from various species, including pets, domestic animals and multiple wild animals, could bind to SARS-CoV-2 receptor binding domain (RBD) and facilitate the transduction of SARS-CoV-2 pseudovirus. Comparing to SARS-CoV-2, SARS-CoV seems to have a slightly wider range in choosing its receptor. We further resolved the cryo-electron microscopy (cryo-EM) structure of the cat ACE2 (cACE2) in complex with the SARS-CoV-2 RBD at a resolution of 3 Å, revealing similar binding mode as hACE2 to the SARS-CoV-2 RBD. These results shed light on pursuing the intermediate host of SARS-CoV-2 and highlight the necessity of monitoring susceptible hosts to prevent further outbreaks.
NT5C2 methylation regulatory interplay between DNMT1 and insulin receptor in type 2 diabetes
Authors: Chen Yng-Tay , Lin Wei-De , Liao Wen-Ling , Tsai Ya-Ching , Liao Jiunn-Wang , Tsai Fuu-Jen 
Published: September 2020

AbstractEpigenetics alternation of non-genetic variation and genome-wide association study proven allelic variants may associate with insulin secretion in type 2 diabetes (T2D) development. We analyzed promoter DNA methylation array to evaluate the associated with increased susceptibility to T2D (30 cases, 10 controls) and found 1,091 gene hypermethylated in promoter regions. We performed the association study of T2D and found 698 single nucleotide polymorphisms in exon and promoter sites by using 2,270 subjects (560 cases, 1,710 controls). A comparison of DNA hypermethylation and gene silencing of mouse T2D results in our T2D patients’ results showed that the 5′-nucleotidase, cytosolic II (NT5C2) and fucosyltransferase 8 (FUT8) genes were strongly associated with increased susceptibility to T2D. DNA hypermethylation in promoter regions reduced NT5C2 gene expression, but not FUT8 in T2D patients. NT5C2 protein expression was decreased in pancreatic β-cells from T2D mice. Transient transfection NT5C2 into RIN-m5F cells down-regulated DNA methyltransferase I (DNMT1) expression and up-regulation of the insulin receptor. Moreover, NT5C2 knockdown induced in DNMT1 overexpression and insulin receptor inhibition. Taken together, these results showed that NT5C2 epigenetically regulated insulin receptor in patients and mice with T2D, and maybe provide for T2D therapy strategy.
Longitudinal micro-computed tomography-derived biomarkers quantify non-resolving lung fibrosis in a silicosis mouse model
Authors: Dekoster Kaat , Decaesteker Tatjana , Berghen Nathalie , Van den Broucke Sofie , Jonckheere Anne-Charlotte , Wouters Jens , Krouglov Anton , Lories Rik , De Langhe Ellen , Hoet Peter , Verbeken Erik , Vanoirbeek Jeroen , Vande Velde Greetje 
Published: September 2020

AbstractIn spite of many compounds identified as antifibrotic in preclinical studies, pulmonary fibrosis remains a life-threatening condition for which highly effective treatment is still lacking. Towards improving the success-rate of bench-to-bedside translation, we investigated in vivo µCT-derived biomarkers to repeatedly quantify experimental silica-induced pulmonary fibrosis and assessed clinically relevant readouts up to several months after silicosis induction. Mice were oropharyngeally instilled with crystalline silica or saline and longitudinally monitored with respiratory-gated-high-resolution µCT to evaluate disease onset and progress using scan-derived biomarkers. At weeks 1, 5, 9 and 15, we assessed lung function, inflammation and fibrosis in subsets of mice in a cross-sectional manner. Silica-instillation increased the non-aerated lung volume, corresponding to onset and progression of inflammatory and fibrotic processes not resolving with time. Moreover, total lung volume progressively increased with silicosis. The volume of healthy, aerated lung first dropped then increased, corresponding to an acute inflammatory response followed by recovery into lower elevated aerated lung volume. Imaging results were confirmed by a significantly decreased Tiffeneau index, increased neutrophilic inflammation, increased IL-13, MCP-1, MIP-2 and TNF-α concentration in bronchoalveolar lavage fluid, increased collagen content and fibrotic nodules. µCT-derived biomarkers enable longitudinal evaluation of early onset inflammation and non-resolving pulmonary fibrosis as well as lung volumes in a sensitive and non-invasive manner. This approach and model of non-resolving lung fibrosis provides quantitative assessment of disease progression and stabilization over weeks and months, essential towards evaluation of fibrotic disease burden and antifibrotic therapy evaluation in preclinical studies.
Short course of voriconazole therapy as a risk factor for relapse of invasive pulmonary aspergillosis
Authors: Shin Dong Hoon , Yoo Seung-Jin , Jun Kang Il , Kim Hyungjin , Kang Chang Kyung , Song Kyoung-Ho , Choe Pyoeng Gyun , Park Wan Beom , Bang Ji-Hwan , Kim Eu Suk , Park Sang Won , Kim Hong Bin , Kim Nam-Joong , Oh Myoung-don 
Published: September 2020

AbstractTo investigate associations of the duration of voriconazole treatment and radiological response with relapse of invasive pulmonary aspergillosis (IPA) in immunocompromised patients, we explored the risk factors for IPA relapse after successful initial treatment. All patients with proven or probable IPA who had finished voriconazole treatment between 2005 and 2019 in a tertiary-care hospital were reviewed. IPA relapse was defined as re-diagnosis of proven or probable IPA at the same site within 1 year after treatment termination. Short course of voriconazole treatment was defined as a treatment less than 9 weeks, which is a median of the recommended minimum duration of therapy from the Infectious Disease Society of America. The radiological response was defined as a reduction in IPA burden by more than 50% on chest computed tomography. Of 87 patients who had completed voriconazole treatment, 14 (16.1%) experienced IPA relapse. Multivariable Cox regression identified that short voriconazole treatment duration (adjusted hazard ratio [aHR], 3.7; 95% confidence interval [CI], 1.1–12.3;P = 0.033) and radiological non-response (aHR, 4.6; 95% CI, 1.2–17.5;P = 0.026) were independently associated with relapse of IPA after adjusting for several clinical risk factors. Longer duration of therapy should be considered for those at higher risk of relapse.
Craniofacial anthropometric investigation of relationships between the nose and nasal aperture using 3D computed tomography of Korean subjects
Authors: Ryu Joon Yeol , Park Ki-Su , Kim Min-Ji , Yun Ji-Su , Lee U-Young , Lee Sang-Seob , Roh Byung-Yoon , Seo Jeong-Uk , Choi Chang-Un , Lee Won-Joon 
Published: September 2020

AbstractThis study investigated the relationships of morphology and locations of the nose and nasal aperture by using major craniofacial landmarks on the human skull and face for craniofacial reconstruction/approximation of Koreans. In the frontal view, the positions of bony landmarks on the skull, including the nasal aperture, were correlated with the positions of nasal landmarks vertical to the transverse plane. In profile, the positions of bony landmarks on the skull were correlated with the positions of nasal landmarks horizontal to the coronal plane. Overall, 26 of the 76 measurements demonstrated significant correlations between the corresponding landmarks on the nose and nasal aperture. Simple regression equations were produced from the results. This study showed that the nose and nasal aperture are significantly related to each other in terms of their morphology and location in Koreans. The prediction guidelines, produced as regression formulas, can be applied to craniofacial reconstruction/approximation and bio-anthropological research of Korean skulls. The study results can also be used clinically in rhinoplasty and nasal reconstruction surgery.
Carbon fiber-reinforced pedicle screws reduce artifacts in magnetic resonance imaging of patients with lumbar spondylodesis
Authors: Fleege Christoph , Makowski Marcus , Rauschmann Michael , Fraunhoffer Katharina Luise , Fennema Peter , Arabmotlagh Mohammad , Rickert Marcus 
Published: September 2020

AbstractThe study investigated whether the use of carbon fiber-reinforced PEEK screw material (CF-PEEK) can reduce magnetic resonance imaging (MRI) artifact formation. Two consecutive groups of patients were treated for degenerative spinal disorders of the lumbar spine with dorsal transpedicular spinal fusion. The first group (n = 27) received titanium pedicle screws. The second group (n = 20) received CF-PEEK screws. All patients underwent an MRI assessment within the first four postoperative weeks. For each operated segment, the surface of the artifact-free vertebral body area was calculated as percentage of the total vertebral body. For each implanted segment, the assessability of the spinal canal, the neuroforamina, and the pedicle screws, as well as the surrounding bony and soft-tissue structures was graded from 1 to 5. A mean artifact-free vertebral body area of 48.3 ± 5.0% was found in the in the titanium group and of 67.1 ± 5.6% in the CF-PEEK group (p ≤ 0.01). Assessability of the lumbar spine was significantly improved for CF-PEEK screws (p ≤ 0.01) for all measurements. CF-PEEK pedicle screws exhibit smaller artifact areas on vertebral body surfaces and their surrounding tissues, which improves the radiographic assessability. Hence, CF-PEEK may provide a diagnostic benefit.