bluuebunny/crossref_metadata_2025_split huggingface dataset:
Molecular Identification and Phylogenic Analysis of Phosphate Solubilizing Bacteria Aneurinibacillus migulanus From Rhizosphere Imperata Cylindrica, Karst Citatah, Bandung Barat, Jawa Barat, Indonesia
Authors: Hafsari A R , Purnawan L 
Published: October 2020

Abstract
Phosphate converted from insoluble phosphate bonds (through secretion of organic acids) to P-inorganic bonds by phosphate solubilizing bacteria (PSB). Two highly potential PSB isolates, BR.5 and BR.7, were isolated from rhizosphere Imperata cylindrica, ex-mining limestone land in Karst Citatah, Province Jawa Barat, Indonesia. From the screening of P-solubilizing test results, BR.5 showed the highest clear zones in pikovskaya agar containing Ca3(PO4)2. The molecular methods to determine the species of bacteria are DNA extraction, DNA amplification by PCR, qualitative DNA testing by electrophoresis, and sequencing. The result of PCR results of DNA bands by electrophoresis, BR.5 has 1500 bp fragment length, 16S rRNA gene yielded on the phylogenic tree of Isolate BR. 5 has a similarity or relationship with the species Aneurinibacillus migulanus Isolate Am25 with a bootstrap value of 99%. Based on the genetic distance between Isolate BR.5 and Aneurinibacillus migulanus, Isolate Am25 has a distance value of 0.71%.
Molecular Study of Root Colonization and Diversity of Arbuscular Mycorrhizal Fungi (AMF) Associated with Lesser Yam (Dioscorea esculenta)
Authors: Nuryana I , Widada J , Subandiyah S 
Published: October 2020

Abstract
Lesser yam (Dioscorea esculenta) can be utilized as a functional food due to the high content of inulin, an ingredient of food products and prebiotics. Hence, it is important to increase and promote yam tuber production by using beneficial microbes such as arbuscular mycorrhizal fungi (AMF). This research was aimed to investigate the root colonization and diversity of AMF associated with lesser yam growing at two altitudes. Soil and root samples were collected from different altitudes, lowlands and highlands. The percentage of root colonization was measured using both the staining method and relative quantification using qPCR. The diversity of AMF was analyzed by using molecular approach T–RFLP with a specific primer pair AML1–AML2 and measured by Shannon–Wiener index. Results showed that root samples from lowlands had a higher percentage of root colonization and significant difference than highlands. A total of identified 17 AMF species belonging to 9 genera: Scutelluspora, Septoglomus, Sclerocystis, Ambispora, Gigaspora, Acaulospora, Claroideoglomus, Funneliformis, and Glomus were determined based on genebank database. Acaulospora was the most dominant and abundant, followed by Glomus and Gigaspora. The study indicated that these genera will be more effective used as potential AMF inoculum to improve lesser yam tuber production.
Determination of optimum conditions to produce stearoyl glycerol enzymatically
Authors: Roosdiana Anna , Mahdi Chanif , Sutrisno  , Rahma Zahza Fatika 
Published: October 2020

Abstract
Stearoyl glycerol is the result of the esterification of glycerol with stearic acid enzymatically using immobilized lipase as catalyst. The lipase is immobilized with chitosan matrix with entrapment method. This study aims were to determine the optimum condition of esterification of glycerol with stearic acid based on reaction time and temperature, and reactant mole ratio. The esterification reaction was carried out by varying reaction time (6, 12, 18, 24, 30) hours, reaction temperature (30, 35, 45, 50, 55) °C, and mole ratio of stearic acid : glycerol (1: 1, 1: 2, 1: 4, 1: 6, 1: 8), the optimum conditions were achieved through conversion percentage. The produced ester was identified by FTIR spectrophotometer and characterized by the value of Hydrophilic-Lipophilic Balance (HLB). The results showed that optimum conditions on reaction time 24 hours, reaction temperature 45°C and mole ratio of stearic acid:glycerol (1:2) produced stearoyl glycerol with conversion percentage of 41.04% and HLB values of 5.01 that was included in the w/o emulsifier type. In addition, the FTIR spectra showed a strong absorption at wave numbers 1703.03 cm−1 (C=O), 1043.02 cm−1(C-O), and 3402.20 cm−1 (O-H) which indicated the characteristic absorption of stearoyl glycerol.
Virtual screening of black rice anthocyanins as antiobesity through inhibiting TLR4 and JNK pathway
Authors: Sari DRT , Safitri A , Cairns JRK , Fatchiyah F 
Published: October 2020

Abstract
Obesity is one of metabolic syndrome and have several complications including type 2 diabetes mellitus and inflammations. To reduce the complications and prevent the obesity, some natural constituents has been tested. This study proved the screening of cyanidin, cyanidin-3-O-glucoside, peonidin and peonidin-3-O-glucoside for inhibiting TLR4 and JNK protein using in-silico study. Four black rice anthocyanins, cyanidin, cyanidin-3-O-glucoside, peonidin and peonidin-3-O-glucoside as ligands taken out from PubChem database. Toll-like receptor 4 (TLR4) and c-Jun NH2-terminal kinase as target protein retrieved from protein data bank. Both of anthocyanins and proteins were docked by Hex 8.0 software and examined using Discovery studio v.19. All black rice anthocyanins interacted with TLR4 and JNK proteins. Cyanidin-3-O-glucoside, cyanidin, peonidin-3-O-glucoside and peonidin inhibit TLR4 in substrate residues site, while those anthocyanins directly inhibited the JNK protein in ATP active sites, gatekeeper residues, and phosphorylation sites. Those data indicated that four kind of anthocyanins that detected on black rice have functional role as anti-obesity. Further in-vivo or in-vitro research are required to support this experiment.
The Effects of ß-glucan Extract from Oyster Mushroom (Pleurotus ostreatus) on Expression of Serum Malondialdehyde in Sprague dawley Rats Induced by HFHF Diet
Authors: Yunita Ema Pristi , Yuniar Astilia Mildha , Kusumastuty Inggita , Maghfirotun Alma , Handayani Dian 
Published: October 2020

AbstractThe obesity prevalence in the world continues to increase yearly, which further cause clinical problems related to metabolic syndrome and lipid peroxidation. This study aims to determine the effect of ß-glucan extract from oyster mushrooms on lipid peroxidation markers, namely serum MDA levels in rats. Therefore,Sprague dawleyrats were divided into four groups, namely the KN group, which was fed with AIN-93M standard diet, the KP group was given the AIN-93M modified HFHF diet, the PI group was fed with AIN-93M modified HFHF + ß-glucan diet 125 mg/kgBW, and the P2 group was given the AIN-93M modified HFHF + ß-glucan diet 375 mg/kgBW. The ß-glucan detection test in oyster mushroom extract used an FTIR spectrophotometer, while the content analysis used the Mega-Calc™ from Megazyme, and also, the MDA levels were determined through the TBARS method. Furthermore, based on FTIR spectrum results, it was proven that oyster mushroom extract contained ß-glucan. The provision of HFHF diet for 14 weeks caused the rats to be pre-obese, resulting in lipid peroxidation due to the free radicals induction. The average Fee index rats at the end of treatment were 294.00 + 6.40 (KN), 292.78 + 6.37 (KP), 291.85 + 9.60 (PI), and 286.88 + 10.60 (P2), with a p value of 0.687. Meanwhile, the average serum MDA level (ng/mF) obtained were 507.833 + 35.95 (KN), 504.184 + 29.17 (KP), 540.397 + 29.80 (PI), and 553.996 + 86.78 (P2), with a p value of 0.001. The values of serum MDA levels that were statistically significant were KN vs P2, KP vs P1, KP vs P2, and P1 vs P2. These results showed that the dose and duration of ß-glucan administered were not sufficient to prevent the lipid peroxidation process.
Preparation of Bi2MoO6@Diatomite composite and its visible light driven reduction of Cr(VI) and removal of tetracycline hydrochloride
Authors: Xiao Liguang , Yang Caixia , Li Genzhuang , Chu Xuefeng , Yan Gang 
Published: October 2020

Abstract
The use of cheap and porous substrates to support visible light photocatalyst is an effective solution to avoid the agglomeration of catalyst. Herein, the Bi2MoO6@Diatomite composite has been prepared through hydrothermal reaction with Bi(NO3)3 and Na2MoO4 as precursors and diatomite as substrate. XRD, SEM, TEM, XPS, and DRS are applied to characterize these composite photocatalysts. The photocatalytic performance of the obtained composites were studied through the Cr(VI) reduction and tetracycline hydrochloride (TC) degradation under visible light irradiation (λ > 400 nm). The photocatalytic experimental results display that the sample with 6% diatomite mass faction (Bi2MoO6@6%DE) has the highest catalytic performance in all materials. In the Cr(VI) solution, the Bi2MoO6@6%DE can reduce 90.1% of Cr(VI) in 60 min, while Bi2MoO6 can only reduce 71.7% of pollutants in the same time. In the TC solution, the Bi2MoO6@6%DE can reduce 74.7% of TC in 120 min, while Bi2MoO6 can only reduce 30.5% of pollutants in the same time. The photocatalytic mechanism was further investigated through the test of active radical capture. The main active radicals in the reduction of Cr(VI) is photogenerated electrons. The ·HO−, ·O2
− and holes (h+) are active free radicals in the removal process of TC, and photogenerated holes (h+) have the greatest influence. This composite has good photocatalytic activity and recyclability, so it has great application potential in waste water treatment.
Predicting enhancer-promoter interactions by deep learning and matching heuristic
Authors: Min Xiaoping , Ye Congmin , Liu Xiangrong , Zeng Xiangxiang 
Published: October 2020

AbstractEnhancer-promoter interactions (EPIs) play an important role in transcriptional regulation. Recently, machine learning-based methods have been widely used in the genome-scale identification of EPIs due to their promising predictive performance. In this paper, we propose a novel method, termed EPI-DLMH, for predicting EPIs with the use of DNA sequences only. EPI-DLMH consists of three major steps. First, a two-layer convolutional neural network is used to learn local features, and an bidirectional gated recurrent unit network is used to capture long-range dependencies on the sequences of promoters and enhancers. Second, an attention mechanism is used for focusing on relatively important features. Finally, a matching heuristic mechanism is introduced for the exploration of the interaction between enhancers and promoters. We use benchmark datasets in evaluating and comparing the proposed method with existing methods. Comparative results show that our model is superior to currently existing models in multiple cell lines. Specifically, we found that the matching heuristic mechanism introduced into the proposed model mainly contributes to the improvement of performance in terms of overall accuracy. Additionally, compared with existing models, our model is more efficient with regard to computational speed.
Effect of high cut-off dialysis for acute kidney injury secondary to cast nephropathy in patients with multiple myeloma: a systematic review and meta-analysis
Authors: Tarragón Blanca , Ye Nan , Gallagher Martin , Sen Shaundeep , Portolés Jose Maria , Wang Amanda Y 
Published: October 2020

ABSTRACT

Background
Acute kidney injury (AKI) caused by cast nephropathy is associated with increased morbidity and mortality among patients with multiple myeloma (MM). High cut-off haemodialysis (HCO-HD) has proven to be effective in the removal of serum light chains but the effect on clinical outcomes, especially renal recovery, remains uncertain.

Methods
A systematic review and meta-analysis were performed examining all randomized controlled trials (RCTs) and observational studies (OBSs) assessing the effect of HCO-HD on clinical outcomes of patients with MM complicated by cast nephropathy–induced severe AKI. The primary outcome was all-cause mortality at the end of the study. The secondary outcomes included all-cause mortality at 12 months, HD independence and serum kappa and lambda light chain reduction. Pooled analysis was performed using random effects models.

Results
We identified five studies, comprising two RCTs and three retrospective cohort studies, including 276 patients with a mean follow-up of 18.7 months. The majority of the studies were of suboptimal quality and underpowered. Compared with patients treated with conventional HD, HCO-HD was not associated with a survival benefit at 12 months {five studies, 276 patients, relative risk [RR] 1.02 [95% confidence interval (CI) 0.76–1.35], I2 = 33.9%} or at the end of the studies at an average of 34 months [five studies, 276 patients, RR 1.32 (95% CI 0.71–2.45), I2 = 62.0%]. There was no difference in HD independence at 90 days [two trials, 78 patients, RR 2.23 (95% CI 1.09–4.55)], 6 months [two studies, 188 patients, RR 1.19 (95% CI 0.68–2.06)] or 12 months [two studies, 188 patients, RR 1.14 (95% CI 0.58–2.26)]. Patients receiving HCO dialysis, however, had a greater reduction in serum kappa [two studies, 188 patients, weighted mean difference (WMD) 46.7 (95% CI 38.6–54.7), I2 =  52.0%] and lambda [two studies, 188 patients, WMD 50.3 (95% CI 21.4–79.3), I2 = 95.1%] light chain levels.

Conclusion
Current evidence from RCTs and OBSs suggests HCO dialysis is able to reduce serum free light chains but makes no significant improvement in all-cause mortality and renal outcomes compared with conventional HD for patients with myeloma cast nephropathy. However, there is a trend towards better renal outcomes with the use of HCO dialysis. The lack of long-term data and the small sample sizes of the included studies limit this analysis. Therefore further large-scale RCTs with longer follow-up are needed to assess the effect of HCO dialysis on clinical outcomes in patients with myeloma cast nephropathy.
Contribution of Larvae Developing on Corn and Dry Beans to the Adult Population of Western Bean Cutworm in Michigan
Authors: Bunn Dakota C , Dias de Oliveira Eduardo , Springborn Frederick , Gonzalez-Meler Miquel A , Miller Nicholas 
Published: October 2020

AbstractThe western bean cutworm, Striacosta albicosta (Smith) (Lepidoptera: Noctuidae), is historically a pest of both corn (Zea mays L. (Poales: Poaceae)) and dry beans (Phaseolus sp. L. (Fabales: Fabaceae)) in the western Great Plains. However, it has recently undergone an eastward range expansion establishing itself across the Corn Belt in 25 states and 4 Canadian provinces. To mitigate the effects of infestation in Michigan, foliar insecticides are used in dry beans, whereas management of the pest in corn relies more heavily on the use of Bt-expressing hybrids. In this study stable carbon isotope analysis was used to determine what crop adult moths developed on as larvae with analysis showing that very few of the adult moths developed on dry beans. These results suggest that beans and corn are not suitable as co-refuges and that mainly adults which developed on corn are contributing to the next generation of western bean cutworm in Michigan.
Carabid Beetle Assemblages (Coleoptera: Carabidae) in a Seminatural Grassland and the Adjacent Old Beech Forest in Northeast Japan
Authors: Okatsu Yutaro , Tsutsumi Tadaaki 
Published: October 2020

AbstractThe decline and disappearance of seminatural grasslands in Japan have caused a reduction in plant and animal species inhabiting such grasslands. We aimed to understand the assemblage structure, species diversity, and distribution of carabid beetles in traditionally managed seminatural grasslands, by comparing with the adjacent old beech forest. We investigated the carabid beetle assemblages in a seminatural grassland maintained by prescribed burning and annual mowing, and the adjacent old beech forest in Yamagata Prefecture, northeast Japan. We recorded several forest species along with open habitat species and habitat generalists in the grassland, suggesting that forest species may utilize the adjacent grasslands as temporary habitats. Cluster analysis showed that the assemblage structure of carabid beetles in the grassland differed from that in the beech forest. There were no clear differences in the carabid assemblages between the burned grassland sites and the grassland sites mowed in July after burning. This suggests that the annual mowing had little influence on the response of grassland carabid species in parts of the grassland. We recorded Harpalus roninus (Coleoptera: Carabidae), a rare carabid species in Japan, indicating that this beetle can be a characteristic of the studied grassland. Redundancy analysis showed that the eight abundant grassland species were associated with canopy openness, grass height, and understory vegetation cover, whereas the five most common species recorded from the beech forest were associated with litter depth and soil moisture.
Scientific publications in internal medicine and family medicine: a comparative cross-sectional study in Swiss university hospitals
Authors: Sebo Paul , de Lucia Sylvain , Vernaz Nathalie 
Published: October 2020

Abstract

Background
Family medicine is a relatively new academic medical discipline. We aimed to compare the main bibliometric indices of hospital-based senior physicians practicing internal medicine versus family medicine in Switzerland.

Methods
We conducted this cross-sectional study in March 2020. We selected all hospital-based senior physicians practicing internal medicine or family medicine in the six Swiss university hospitals. Using Web of Science, after removing from both groups of physicians the 5% with the highest number of publications, we extracted the number of publications, the number of publications per year, the number of citations, the number of citations per year, the number of citations per publication and the h-index. We compared the data between the two groups using negative binomial regressions and the proportion of physicians having at least one publication using chi-square tests.

Results
We included 349 physicians in the study (internal medicine: 51%, men: 51%). The median number of publications was three [interquartile range (IQR) = 18], the median number of citations was nine (IQR = 158) and the median h-index was one (IQR = 5). All bibliometric indices were similar in both groups, as was the proportion of physicians having at least one publication (family medicine: 87% versus 82%, P = 0.15).

Conclusions
We found no association between the bibliometric indices and the medical specialty. Further studies are needed to explore other important indicators of academic output, such as those more specifically assessing its quality and scientific importance.
Antecedents and Outcomes of Enrichment Among Working Family Caregivers of People With Dementia: A Longitudinal Analysis
Authors: Morimoto Hiroshi , Takebayashi Yoshitake 
Published: October 2020

Abstract

Objectives
Despite evidence of negative aspects of the work–caregiving interface (e.g., work–family conflict) among family caregivers of people with dementia (PWD), little is known about the positive aspects (e.g., enrichment). We examined antecedents and outcomes of family-to-work enrichment (FWE) and work-to-family enrichment (WFE) among working family caregivers of PWD. In terms of antecedents, we investigated whether factors that alleviated work–family conflict increased enrichment.

Method
We conducted a 3-wave 6-month-interval longitudinal online survey of Japanese working family caregivers of PWD (N = 747). We examined the mediational effects of WFE and FWE on associations between participants’ work resources (job control, supervisor support, co-worker support, and organizational support) and caregiving support and their well-being (psychological distress and quality of life). We also examined the moderating effect of caregiving self-efficacy on the relationships between caregiving support/caregiving demands and FWE.

Results
Our longitudinal analysis confirmed supervisor support had a positive effect on WFE. FWE had no significant longitudinal mediating effect on the association between caregiving support and well-being, and self-efficacy had no longitudinal moderating effect on FWE.

Discussion
Supervisor support is important for WFE, but greater enrichment does not necessarily improve family caregiver well-being. Caregiving experience (i.e., caregiving demands and caregiving support) has little effect on the work–caregiving interface. Policy makers should focus on supporting companies to create family-friendly work environments. More research is needed on factors that increase FWE and moderate the relationship between enrichment and working family caregivers’ well-being.
Cognition in Motion: Evidence for Intact Action Control With Healthy Aging
Authors: Ruitenberg Marit F L , Koppelmans Vincent 
Published: October 2020

Abstract

Objectives
Healthy aging is associated with impairments in motor functioning. Such functioning is not limited to the physical execution of actions, but also involves cognitive processes that allow for goal-directed behavior. The present study examined whether aging affects 2 of such cognitive components that control motor functioning, namely action planning and action adaptation, and whether age effects are associated across components.

Method
A group of 103 participants aged 18–82 years performed 2 tasks that have previously been linked to action planning and adaptation, respectively.

Results
Despite observations that aging was associated with slower and less accurate responses, Bayesian models showed evidence indicating that older age was not associated with poorer action planning and conflict adaptation.

Discussion
These findings challenge the view that healthy aging is associated with a general deficit in motor functioning and suggest that some cognitive aspects of motor control may be relatively spared.
Constitutional rights, horizontality, and the Ugandan Constitution: An example of emerging norms and practices in Africa
Authors: Chirwa Danwood M , Mbazira Christopher 
Published: October 2020

Abstract
The traditional view that only states have human rights obligations and, relatedly, that a constitution operates only vertically has been changing. In Uganda, as is the case in several other African countries, the Constitution expressly states that the rights and freedoms it recognizes must be respected, upheld, and promoted by all persons and organs of state, but the practical implications of this provision remain unexplored. So far, Ugandan courts have shown willingness to hold non-state actors directly responsible for violations of constitutional rights, although they are yet to use the language of horizontality expressly. Furthermore, Ugandan courts do not seem particularly troubled by the principle of subsidiarity which holds that statutory and common law remedies must be pursued first before recourse may be had to direct constitutional remedies. This has made it possible for litigants to bring direct constitutional suits against state and non-state actors in one action or to plead both constitutional provisions and statutory provisions, or direct and indirect horizontality, to enforce constitutional rights. This is a significant departure from the existing practice of constitutional horizontality in comparative constitutional law. In other respects, however, such as recognizing the duty to protect and applying the principle of third-party effect of constitutional rights, Uganda’s constitutional jurisprudence remains underdeveloped.