bluuebunny/crossref_metadata_2025_split huggingface dataset:
Differential functions of FANCI and FANCD2 ubiquitination stabilize ID2 complex on DNA
Authors: Rennie Martin L. , Lemonidis Kimon , Arkinson Connor , Chaugule Viduth K. , Clarke Mairi , Streetley James , Spagnolo Laura , Walden Helen 
Published: February 2020

AbstractThe Fanconi Anemia (FA) pathway is a dedicated pathway for the repair of DNA interstrand crosslinks, and which is additionally activated in response to other forms of replication stress. A key step in the activation of the FA pathway is the monoubiquitination of each of the two subunits (FANCI and FANCD2) of the ID2 complex on specific lysine residues. However, the molecular function of these modifications has been unknown for nearly two decades. Here we find that ubiquitination of FANCD2 acts to increase ID2’s affinity for double stranded DNA via promoting/stabilizing a large-scale conformational change in the complex, resulting in a secondary “Arm” ID2 interphase encircling DNA. Ubiquitination of FANCI, on the other hand, largely protects the ubiquitin on FANCD2 from USP1/UAF deubiquitination, with key hydrophobic residues of FANCI’s ubiquitin being important for this protection. In effect, both of these post-translational modifications function to stabilise a conformation in which the ID2 complex encircles DNA.
A Guide to the Quantitative Proteomic Profiles of the Cancer Cell Line Encyclopedia
Authors: Nusinow David P. , Gygi Steven P. 
Published: February 2020

AbstractWe recently reported the quantitative proteomics of 375 samples as part of the Cancer Cell Line Encyclopedia (Nusinow et al., 2020). Mass spectrometry-based proteomics data is broadly unfamiliar to most biologists in our experience, resulting in questions from analysts about how to use the data. From the proteomics community there was interest about how we normalized the data, as the scope of this project was so much larger than what has been commonly available. This paper serves as a guide to the data set to answer these questions and acts as a supplement to the main manuscript. The first part addresses users of the data, describing the experimental design, interpretation of the values, and dealing with standard issues in proteomics like multiple protein isoforms per gene and missing values. The second part of the manuscript details how we arrived at the normalization procedure reported in the paper, including the diagnostics used to assess multiple normalization schemes.
Altered conformational sampling along an evolutionary trajectory changes the catalytic activity of an enzyme
Authors: Kaczmarski Joe A. , Mahawaththa Mithun C. , Feintuch Akiva , Clifton Ben E. , Adams Luke A. , Goldfarb Daniella , Otting Gottfried , Jackson Colin J. 
Published: February 2020

AbstractSeveral enzymes are known to have evolved from non-catalytic proteins such as solute-binding proteins (SBPs). Although attention has been focused on how a binding site can evolve to become catalytic, an equally important question is: how do the structural dynamics of a binding protein change as it becomes an efficient enzyme? Here we performed a variety of experiments, including double electron-electron resonance (DEER), on reconstructed evolutionary intermediates to determine how the conformational sampling of a protein changes along an evolutionary trajectory linking an arginine SBP to a cyclohexadienyl dehydratase (CDT). We observed that primitive dehydratases predominantly populate catalytically unproductive conformations that are vestiges of their ancestral SBP function. Non-productive conformational states are frozen out of the conformational landscapeviaremote mutations, eventually leading to extant CDT that exclusively samples catalytically relevant compact states. These results show that remote mutations can reshape the global conformational landscape of an enzyme as a mechanism for increasing catalytic activity.
Optimal design methods for the uniform heating of tube ends for stress relieving
Authors: Aliferov Alexander , Di Barba Paolo , Dughiero Fabrizio , Forzan Michele , Lupi Sergio , Mognaschi Maria Evelina , Sieni Elisabetta 
Published: February 2020

Purpose
An inductor for the uniform heating of the extremity of a ferromagnetic steel tube for stress relieving is considered. The main goal of the study is to investigate the possibility to achieve a reasonable design of the inductor when dealing with many design variables.

Design/methodology/approach
Genetic optimization algorithms are used for this purpose, demonstrating the applicability of these techniques to the design of induction heating inductors. Genetic algorithms provide to the designer several optimal solutions belonging to Pareto Front, and this way they allow choosing the solution that better fits the technological requirements. In any case, the designer has to adapt the chosen solution to fit in with the real possibilities in industrial application.

Findings
The study demonstrates that automatic optimization methods may help the designer of the induction heating system to solve complex problems with very conflicting technological requirements.

Originality/value
In the paper, a problem with a high number of design variables is solved. Moreover, the goals of the optimization process are strongly conflicting, and the proposed problem is a challenging one.
The role of customer engagement in travel services
Authors: Parihar Prateeksha , Dawra Jagrook 
Published: February 2020

Purpose
The purpose of this study is to understand the role of customer engagement (CE) in transforming a committed customer into a loyal customer in an online service context. The study explores the varied effect of the dimensions of commitment on the dimensions of beyond purchase CE and their effect on loyalty.

Design/methodology/approach
The study uses a survey for data collection and assesses the measurement model with confirmatory factor analysis and uses partial least square-structural equation modeling for the structural model.

Findings
The study reveals that the constructs commitment, CE and loyalty are interrelated. It empirically supports that the affective and continuance commitment has a significant positive association with CE, and two out of the three dimensions of CE have a significant positive association with loyalty.

Research limitations/implications
This study advances the understanding of the relationship between commitment, CE and loyalty and supports the conceptualization of CE as a behavioral construct. The study uses cross-sectional data, which miss the effect of the time lag of the constructs and use of the context of travel booking websites limits its generalization.

Practical implications
This study helps marketers to understand CE, what motivates CE, its impact on the firm and how to align CE strategies with the firm’s goal.

Originality/value
This study proposes a conceptual model for how CE transforms commitment into loyalty. It improves the understanding of CE’s conceptualization, its drivers and its impact on brand loyalty.
How to boost place branding leveraging on community relations
Authors: Palazzo Maria , Deigh Linda , Foroudi Pantea , Siano Alfonso 
Published: February 2020

Purpose
This paper aims to explore the relationship between community relations (CR) and the concept of place branding (PB) by analysing several companies in a developing sub-Saharan country – Ghana, and developing a framework that links the selected concepts together.

Design/methodology/approach
The paper provides an analysis based on a multiple case study approach, with an interpretivist analysis of secondary and primary data derived from archival documents and in-depth interviews of corporate social responsibility (CSR) and brand managers from purposefully selected cases of private financial institutions. The data gathered were qualitatively analysed to identify and interpret common themes about CR, PB and other relevant factors such as culture. Based on analysis of the qualitative data, a comprehensive framework for CR and PB was formulated.

Findings
Findings show that in the developing sub-Saharan country Ghana, the process of establishing a place brand is complex because of influences exerted on CR practices by culture, management agenda of private organisations, government intervention and the fragmentation of efforts to generate a coherent dialogue with numerous stakeholders.

Practical implications
The study shows that managers can leverage on an array of CR elements, including moral and ethical obligations of the company, provision of economic benefits, integration, common goals between the corporation and its communities, responsibility to stakeholders, proactive action, partnerships across sector lines and active leadership, to boost PB. Thus, this research will help policymakers, country brand managers and communication professionals in structuring a proper PB starting from the efforts made in the CR field.

Originality/value
This research can be considered one of the few studies undertaken with a view to understanding and developing a CR framework that links with PB in a developing country. The study identifies several important moderators of PB and factors influencing CR. All issues are approached from the study of PB that promotes economic, commercial and political interests at home and abroad.
Installation strategy and optimization of PM sensor for diesel engine
Authors: Tang Dong , Wang Li , Liu Yang , Liu Ning , Wu Yuzhe , Chen Lie 
Published: February 2020

Purpose
This paper aims to design and optimize the threaded fastener of leakage current particulate matter (PM) sensor. The corresponding air-tight test is conducted to ensure the reliability of the installation strategy with screw connection.

Design/methodology/approach
Research on the pressure-deformation curve of seal gasket was conducted and the vibration load of engine was considered for the calculation of the minimum installation pre-tightening force. Simultaneously, the danger threaded section area was calculated, and the carrying capacity was verified. The height of the welding line was studied to ensure the reliability of the application. FEA was carried out to acquire the relationship between local structure size and local stress for continuous improvement of thread connection. The installation torque range was acquired from the torque control principle for the pre-tightening force. The sealing reliability of the connector was proved with leakage.

Findings
The air tightness of the thread connector is proved to be fine. When the pre-tightening force is over 8,000 N, and its length reaches 2 mm, the connector has good reliability at ambient temperature. The tightening torque of 60-74 Nm can guarantee the reliable fixing ability of thread connector, and its plastic non-deformation ability in the process of repeated tearing down.

Originality/value
This paper provides an installation strategy and an optimization of PM sensor, which has a positive effect on the study and the manufacture of PM sensor. It is helpful to further develop PM sensor and after-treatment technology. This kind of real-time monitoring PM sensor needs to be studied further to achieve its commercial application.
Melting pot or salad bowl: Cultural distance and housing investments
Authors: Deng Yongheng , Hu Maggie R. , Lee Adrian D. 
Published: February 2020

AbstractThis paper investigates whether individuals’ cultural background affects their housing investment decisions, drawing inferences from property location choices, and transaction prices in Australia. We propose a novel measure of cultural distance for each homebuyer–neighborhood pair, adopting the six‐dimensional cultural framework of Hofstede. Our measure captures interethnicity similarity and thus measures the cultural similarity of each buyer and neighborhood pair at a more granular level. Utilizing housing transaction data of a culturally diverse city Sydney, Australia, we find that buyers are more likely to buy properties in neighborhoods with a shorter cultural distance to their culture of origin and are willing to pay a premium for properties in those locations, consistent with buyers’ preference for cultural proximity. Further, we show that cultural proximity preference is stronger for ethnicities from recent migration waves, particularly Asia. Our findings are generalizable to other regions with ethnic aggregations and offer new insights into the growing literature on culture and household finance as well as the urban economics literature.
Pre‐embedded cervical circular stapled anastomosis in esophagectomy
Authors: Li Jie , Wang Bin , Liang Tao , Guo Nan‐nan , Zhao Ming 
Published: February 2020

AbstractBackgroundMechanical anastomosis is now widely used in surgery for esophageal cancer. An anastomotic fistula is still the most dangerous complication in mechanical anastomosis, especially for patients who undergo cervical anastomosis. However, due to the high anastomosis position and limited space, conventional embedding and suspension are rarely performed. This study aimed to introduce the steps of an improved embedded method in cervical circular stapled anastomosis and evaluate its efficacy in reducing complications.MethodsIn total, 31 patients who underwent minimally invasive esophagectomy were enrolled into the study. Pre‐embedded cervical esophagogastrostomy with a circular stapler was adopted after thoracoscopic and laparoscopic esophagectomy for esophageal cancer.ResultsThe results of surgical duration, blood loss, mean duration of hospitalization and operation complications such as anastomotic fistula, anastomotic stenosis and gastroesophageal reflux were recorded. The operative procedure lasted between 205–300 minutes with an average of 260.3 minutes. The postoperative recovery was good, with no complications such as anastomotic fistula, anastomotic stricture and pulmonary complication, except for two cases of gastroesophageal reflux. The postoperative hospital stay was 8–14 days with an average of 10.3 days.ConclusionOur data revealed that pre‐embedded cervical circular stapled anastomosis is an alternative for patients with good stomach length, which can decrease the occurrence rate of anastomotic fistula by full peripheral embedding of anastomotic stoma.Key points
This new technique can significantly reduce the risk of anastomotic leakage.
This study adds further details enabling a smooth pre‐embedded procedure to be performed.
Genetic diagnosis and sperm retrieval outcomes for Chinese patients with congenital bilateral absence of vas deferens
Authors: Wang Hongxiang , An Miao , Liu Yidong , Hu Kai , Jin Yan , Xu Shiran , Chen Bin , Lu Mujun 
Published: February 2020

AbstractBackgroundCongenital bilateral absence of the vas deferens (CBAVD) is a frequent cause of obstructive azoospermia. CBAVD is mainly caused by mutations in the CFTR (cystic fibrosis transmembrane conductance regulator) gene and is also related to the X‐linked ADGRG2 (adhesion G protein‐coupled receptor G2) gene. Genetic screening and counseling strategies for Chinese CBAVD populations remain controversial because the genetic background of CBAVD in Chinese population is largely unknown.ObjectivesIn this study, we aimed to study the mutation spectrum of CFTR and ADGRG2 in a group of CBAVD patients and to evaluate sperm retrieval outcomes in a subset of CBAVD patients.Materials and methodsNext‐generation targeted sequencing was used to identify mutations in the CFTR and ADGRG2 genes in 38 CBAVD patients. In addition, we followed and analyzed nine of the 38 patients who were undergoing sperm retrieval surgery.ResultsIn total, 27 of 38 (71.05%) patients carried at least one likely pathogenic or pathogenic mutation in CFTR or ADGRG2. In addition to the IVS9‐5T allele, 15 CFTR and 1 ADGRG2 mutations were identified, including 4 novel mutations. CFTR hot‐spot mutations were not identified in our study. Spermatozoon was successfully obtained in all nine patients who underwent MESA or TESE surgery, but most patients had spermatozoa with relatively low motility and high abnormality rates.Discussion and conclusionExcept for the IVS9‐5T allele, hot‐spot mutations of CFTR may not exist in Chinese CBAVD patients. Therefore, next‐generation targeted sequencing for whole CFTR and ADGRG2 gene may be the appropriate genetic testing method, and genetic counseling may be different from Caucasian populations. We observed a high success rate of sperm retrieval with relatively low motility and high abnormality rates in Chinese CBAVD patients. However, this is only a weak conclusion due to the small sample size.
Comparative study of tapered versus conventional cylindrical balloon for stent implantation in stenotic tapered artery
Authors: Shen Xiang , Jiang Jiabao , Zhu Hongfei , Lu Kaikai , Dong Pengfei , Gu Linxia 
Published: February 2020

AbstractThe natural tapering of coronary arteries often creates a dilemma for optimal balloon sizing during stenting. The influence of different balloon types, namely, a tapered balloon and a conventional cylindrical balloon, on the mechanical performance of the stent as well as arterial mechanics was investigated via the finite element method. Stent free‐expansion and stent deployment in a stenotic tapered artery were investigated numerically. The biomechanical behavior of the two balloon types was compared in terms of stent foreshortening, stent deformation, stent stress distribution, and arterial wall stress distribution. Results indicate that balloon types affect the transient behavior of the stent and the arterial mechanics. Specifically, a tapered balloon could maintain the natural tapering of the coronary artery after stent expansion. In contrast to a cylindrical balloon, tapered balloon also mitigated the foreshortening of the stent (7.69%) as well as the stress concentration in the stent and artery (8.61% and 4.17%, respectively). Hence, tapered balloons should be used in tapered arteries as they may result in low risk of artery injury. This study might provide insights for improved balloon choice and presurgical planning.
Termite mounds house a diversity of taxa in oil palm plantations irrespective of understory management
Authors: Hood Amelia S. C. , Pashkevich Michael D. , Dahlsjö Cecilia A. L. , Advento Andreas D. , Aryawan Anak Agung Ketut , Caliman Jean‐Pierre , Naim Mohammad , Head Jason J. , Turner Edgar C. 
Published: February 2020

AbstractWe investigated the effects of oil palm understory vegetation management on termite mound activity and non‐termite inhabitants. We found a diversity of taxa, most of which were unaffected by understory management. Mound volume and termite activity had taxa‐specific effects on abundance. Preserving mounds in oil palm plantations will benefit biodiversity.Abstract in Indonesian is available with online material.
Integral chain management of wildlife diseases
Authors: Martel An , Vila‐Escale Mireia , Fernández‐Giberteau Daniel , Martinez‐Silvestre Albert , Canessa Stefano , Van Praet Sarah , Pannon Pep , Chiers Koen , Ferran Albert , Kelly Moira , Picart Mariona , Piulats Dolors , Li Zhimin , Pagone Viviana , Pérez‐Sorribes Laia , Molina Carolina , Tarragó‐Guarro Aïda , Velarde‐Nieto Roser , Carbonell Francesc , Obon Elena , Martínez‐Martínez Diego , Guinart Daniel , Casanovas Ricard , Carranza Salvador , Pasmans Frank 
Published: February 2020

AbstractThe chytrid fungus Batrachochytrium dendrobatidis has caused the most prominent loss of vertebrate diversity ever recorded, which peaked in the 1980s. Recent incursion by its sister species B. salamandrivorans in Europe raised the alarm for a new wave of declines and extinctions in western Palearctic urodeles. The European Commission has responded by restricting amphibian trade. However, private amphibian collections, the main end consumers, were exempted from the European legislation. Here, we report how invasion by a released, exotic newt coincided with B. salamandrivorans invasion at over 1000 km from the nearest natural outbreak site, causing mass mortality in indigenous marbled newts (Triturus marmoratus), and posing an acute threat to the survival of nearby populations of the most critically endangered European newt species (Montseny brook newt, Calotriton arnoldi). Disease management was initiated shortly after detection in a close collaboration between policy and science and included drastic on site measures and intensive disease surveillance. Despite these efforts, the disease is considered temporarily contained but not eradicated and continued efforts will be necessary to minimize the probability of further pathogen dispersal. This precedent demonstrates the importance of tackling wildlife diseases at an early stage using an integrated approach, involving all stakeholders and closing loopholes in existing regulations.
Sarcoidosis with cutaneous perineural granulomas and neurological manifestations: A potential mimicker of leprosy
Authors: Kazi Rashek , Kazlouskaya Viktoryia , Ho Jonhan , Karunamurthy Arivarasan 
Published: February 2020

AbstractSarcoidosis is a granulomatous condition with diverse clinical presentations, including neurological findings. It was previously hypothesized that perineural sarcoidal granulomas in the skin may be an explanation of small‐fiber neuropathy. Herein, we present a case of a 55 year old female with anesthetic cutaneous lesions mimicking leprosy clinically and histopathologically and discuss the importance of this differential diagnosis.
Incretin hormones, insulin, glucagon and advanced glycation end products in relation to cognitive function in older people with and without diabetes, a population‐based study
Authors: Dybjer E. , Engström G. , Helmer C. , Nägga K. , Rorsman P. , Nilsson P. M. 
Published: February 2020

AbstractAimThe aim of this observational study was to investigate relationships between physiological levels of glucometabolic biomarkers and cognitive test results in a population‐based setting.MethodsCross‐sectional data were obtained from the Swedish population‐based Malmö Diet and Cancer Study Re‐examination 2007–2012 comprising 3001 older people (mean age 72 years). Through oral glucose tolerance testing (OGTT), fasting and post‐load levels of serum insulin, plasma glucagon, serum glucose‐dependent insulinotropic peptide (GIP) and plasma glucagon‐like peptide‐1 (GLP‐1) were measured. Insulin resistance and insulin sensitivity levels were calculated. In 454 participants, advanced glycation end products (AGEs) were estimated through skin autofluorescence. Associations between biomarkers and two cognitive tests, the Mini‐Mental State Examination (MMSE) and A Quick Test of Cognitive Speed (AQT) respectively, were explored in multiple regression analyses.ResultsPositive associations following adjustments for known prognostic factors were found between MMSE scores and insulin sensitivity (B = 0.822, P = 0.004), 2‐h plasma glucagon (B = 0.596, P = 0.026), 2‐h serum GIP (B = 0.581, P = 0.040) and 2‐h plasma GLP‐1 (B = 0.585, P = 0.038), whereas negative associations were found between MMSE scores and insulin resistance (B = −0.734, P = 0.006), fasting plasma GLP‐1 (B = −0.544, P = 0.033) and AGEs (B = −1.459, P = 0.030) were found.ConclusionsHigher levels of insulin sensitivity, GIP and GLP‐1 were associated with better cognitive outcomes, but AGEs were associated with worse outcomes, supporting evidence from preclinical studies. Glucagon was linked to better outcomes, which could possibly reflect neuroprotective properties similar to the related biomarker GLP‐1 which has similar intracellular properties. Longitudinal and interventional studies are needed to further evaluate neuromodulating effects of these biomarkers.Abstract presented at the European Association for the Study of Diabetes (EASD) 2019, Barcelona, Spain