bluuebunny/crossref_metadata_2025_split huggingface dataset:
Strategic Targeting of CENPE-PDL1 axis in NSCLC
Authors: Liang Jinyan , Tian Chen , Yang Qifan , Gu Feifei , Pi Guoliang , Liu Li , Zhang Yong 
Published: November 2020

Abstract
Background Increasing evidence suggests that centromere-associated protein E (CENP-E) is expressed during mitosis and plays a key role in incorrect chromosome alignment. Therefore, CENP-E may represent a druggable target for several solid tumors. Methods Here, we evaluated the ability of the CENPE inhibitor GSK923295 to up-regulate PDL1 and induce immune responses to tumor-associated CD8 T cell and regulatory T (Treg) cell.Results Our study found that a CENP-E inhibitor exhibited anti-tumor activity by directly suppressing the proliferation of lung cancer cells and upregulating the expression of PD-L1. Inhibition of CENP-E suppressed antitumor immunity by attenuating the response of activated CD8+ T cells and augmenting Tregs in vitro and in vivo. Mechanistically, CENP-E bound to the TTP promoter to regulate its transcription, and inhibition of CENP-E stabilized the mRNA of PD-L1 via TTP targeting of the 3’UTR. Inhibition or knockdown of CENP-E combined with an anti-PD-L1 antibody rescued the impaired antitumor CD8+ T/Treg cell response and improved the antitumor effect in lung cancer. Surprisingly , further analysis found that CENP-E was only related to the poor prognosis of lung cancer. Conclusions All of these results suggest that a CENP-E inhibitor will exert anti-tumor effects and upregulate PD-L1-induced impairment of anti-tumor CD8+/Treg cell responses in lung cancer. However, elevated PDL1 levels provide a possible strategy for combination immunotherapy, and this combination of immunotherapeutic strategies may offset the negative effects of a CENP-E inhibitor on its immune-mediated antitumor ability in lung cancer treatment.
Proteomics Analysis of the Cerebellum in Spontaneously Hypertensive Rats Treated with Twirling Reinforcing Manipulation, Twirling Reducing Manipulation or Electroacupuncture
Authors: Zhang Xu-dong , Hao Xiao-min , Liang Jing-rong , Wu Jiao-juan , Xue Yan-jun , Wu Xiao-li , Wei Peng-cheng , Wang Yu , Xiao Min , Zhao Ji-ping , Liu Qing-guo 
Published: November 2020

Abstract
Purpose: In this experiment, spontaneously hypertensive rats (SHR) were used to further explore the antihypertensive effect of acupuncture twirling reinforcing and reducing manipulation and the central effect mechanism of regulating blood pressure. Methods: 32 male SHR rats were randomly divided into 4 groups: twirling reinforcing manipulation group (RF), twirling reducing manipulation group (RD), electroacupuncture group (EA) (n = 8) and model group (M) (n = 8) and 8 WKY rats were used as blank control group (WKY). Group RD, RF and EA SHR took bilateral Taichong(LR3) point for acupuncture twirling method or electroacupuncture treatment, while WKY and M group did not use any acupuncture manipulation, but only carried out the same grasping and fixation. The differential proteins in cerebellum were screened by proteomic technology, and the key proteins and pathways involved in the pathogenesis of hypertension were selected.Results: RD, RF and EA lowered the blood pressures of the SHRs by varying degrees. The results of Parallel reaction monitoring(PRM) verification showed that among all the differential proteins, 13 differentially expressed proteins (DEPs) were selected for PRM verification. The KEGG annotations of differential proteins are involved in the regulation of lipolysis, autophagy, gap junction, phosphate inositol metabolism, splicing, lysosome, thermogenesis and RNA transport in adipocytes, etc. Conclusion: The results of the current study demonstrated that RD, RF and EA manipulations at the LR3 point reduced blood pressure and that RD was the most effective of the techniques used. Different acupuncture techniques have different pathways for screening and activating cerebellar differential proteins.
Impact of School-Based Health Education Intervention on the Incidence of Soil-transmitted Helminths in Pupils of Rural Schools, Kogi East, North Central Nigeria.
Authors: Yaro Clement Ameh , Kogi Ezekiel , Luka Sodangi Abdulkarim , Kabir Junaidu , Opara Kenneth Nnamdi , Batiha Gaber El-Saber , Nasif Omaima , Alharbi Sulaiman Ali , Alabi Albert Bamigbade , Yunusa Salamat Ibrahim 
Published: November 2020

Abstract
Background: The negative impact of soil-transmitted helminths (STHs) in Nigeria is enormous posing serious public health issues. This study was undertaken to investigate the impact of health education intervention on re-infection of STHs in pupils of rural schools of Kogi East, North Central Nigeria. Methods: A cross-sectional survey was carried out in 45 schools to determine the prevalence of STHs in the 9 local government areas of Kogi East. Stool samples were collected and examined for STHs. A total of 10 schools with the highest prevalence were selected for the follow-up study, 5 schools were dewormed and given health education (DHE) intervention while the other 5 schools were dewormed only (DO). Reassessment of schools for re-infection was carried out for a period of 12 months. Data obtained were analyzed using descriptive statistics. Student t-test was used to make comparison between interventions in the incidence of infections. Analysis was carried out at p<0.05.Results: Re-infection with STHs was observed from the 7th month of both interventions. In the 36th week (9th month), incidence observed in schools given DHE schools (4.79%, 8 pupils) were higher than in DO schools (3.19%, 5 pupils), no significant difference (t = -0.840, p = 0.426) between the interventions. Also, at the 48th week (12th month), no significant difference (t = -0.346, p = 0.738) between the DHE schools (7.19%, 12 pupils) and DO schools (6.37%, 10 pupils). Hookworms had the highest incidence in DHE (6.6%, 11 pupils) and DO (6.4%, 10 pupils) schools among the STHs. A. lumbricoides incidence was low and was observed in a school given DHE (0.6%, 1 pupil). S. stercoralis was not observed throughout. At 48th (12th month), an incidence of 6.37% was observed compared to 32.03% prevalence at baseline in the DO schools and an incidence of 7.11% and prevalence of 36.09% in the DHE schools. Significant difference (p<0.05) exist between baseline and intervention.Conclusion: Non-dewormed individuals at the community levels may have contributed to the poor performance of health education. Community-based deworming should be encouraged alongside improvement in the water, sanitation and hygiene infrastructures at both school and home.
Effect of Drying Method and Hydrothermal Treatment on Physicomechanical Properties of Parboiled Rice
Authors: Mahfeli Mandana , Zomorodian Aliasghar , Zarein Mohammad 
Published: November 2020

Abstract
Rice grain was obtained from milled paddy, in which the husk and bran are removed. During the milling process, rice varieties having inherent poor milling quality or those have been processed under unfavorable conditions, break and so their head rice yield (HRY) decrease. Parboiling is one of the hydrothermal postharvest treatment before drying and milling processes. After parboiling, the grains become stronger and breakage of rice is very much reduced during milling, leading to often a remarkable increase in the HRY. In this study the paddy was soaked in hot water (70 °C) for 1 h and drained, then steaming was done at four periods (0, 5, 10 and 15 minutes) at atmospheric pressure. Drying process was carried out using two driers techniques, solar and continuous, at three inlet air temperature to the chamber dryer of 35, 40 and 45○C. Results revealed that the effects of drying air temperature and steaming time showed a significant effect on HRY and broken kernels for Hashemi rough rice cultivar (p<0.05) in continuous dryer. In both drying techniques the higher HRY performance was appeared at 35○C drying air temperature and 10 min steaming time. It was observed that dried kernels in continuous dryer were stronger (more bending strength) than those dried in the solar dryer and had a higher rate of HRY during subsequent processing operations. It was proved that increasing the steaming time to a safe level caused a reduction in broken kernels which was associated with higher HRY.
Direct Anterior Versus Anterolateral and Posterior Approaches to Total Hip Replacement: Comparative Accuracy of Acetabular Implant Placement and Early Clinical Outcomes
Authors: Chen Ying-Lin , Horng Chi-Horng , Hsieh Shang-Lin , Hsu Chin-Jung , Chang Chien-Chun , Fong Yi-Chin , Chen Hsien-Te , Su Li-Ting , Lin Tsung-Li , Tsai Chun-Hao 
Published: November 2020

Abstract
BackgroundDifferent surgical approaches used in total hip arthroplasty (THA) include a direct anterior approach (DAA), anterolateral approach (AL), and posterolateral approach (PL). However, the acetabular cup position varies according to surgical view, surgical table, and patient position for each approach. This study is aimed to compare acetabular cup position in THA under different approaches, including surgical time, blood loss, and postoperative complications.MethodsBetween Jan 2017 and Dec 2018, 231 patients who underwent THA (64 DAA, 96 AL, and 71 PL THAs) were analyzed retrospectively. Intraoperative blood loss, operation time, preoperative and postoperative WOMAC score, cup anteversion, inclination angle, and postoperative complications were analyzed.ResultsDAA showed longer operation time and more blood loss, but shorter hospital stays. The cup was found in the safe zone for 97% of DAA patients, 74% of AL patients, and 56% of PL patients. PL showed the highest complication rate (9.9%), followed by DAA (3.1%) and AL (1%). There was no statistically significant difference in preoperative and postoperative WOMAC scores. ConclusionTHA by DAA using a special table is a more reliable procedure to achieve safe cup position. Although DAA showed fewer outliers in cup position, it resulted in longer operation time and greater blood loss compared to other groups.Trial registration: Retrospective study
Intestinal Ostomies Created During Emergency Surgery Possess Unique Challenges
Authors: Schulte Wibke , Aliyeva Ilhamiyya , Knoop Michael , Pratschke Johann 
Published: November 2020

Abstract
Background: The surgical creation of an artificial opening of the bowel, called ostomy, can become necessary for very different causative diseases. A special subgroup are ostomies created during emergency surgery, which pose particular challenges to affected patients. This work is dedicated to their detailed characterization.Methods: A retrospective analysis of surgical ostomy creations at an acute care university hospital and an online survey for patients with an ostomy were performed and evaluated.Results: In our study, about one third of all ostomies were created during emergency surgery (37.4%). Compared to patients who received an ostomy during elective surgery, emergency patients had a higher ASA score and diagnoses requiring acute surgical care. Patients undergoing emergency surgery were more likely to have inadequate preoperative medical education (60% vs. 33.3%, p=0.029), and rarely received preoperative ostomy marking (4% vs. 79.2%, p<0.001). Emergency patients underwent minimally invasive surgery less frequently (26.8% vs. 51.3%, p=0.001), and showed a higher rate of peristomal wound dehiscence (9.9% vs. 2.5%, p=0.028). Accordingly, emergency ostomies often resulted in an overall reduction in postoperative quality of life.Conclusion: Ostomies are often created during emergency surgery under suboptimal perioperative conditions. This results in higher complication rates and negative physical and psychological effects. Therefore, intensive interdisciplinary care is essential to provide the best possible care for patients affected by these artificially created intestinal outlets.
New treatment protocol for pseudosyndactyly in epidermolysis bullosa hand using a dermal regeneration template glove.
Authors: LEMBO FEDELE , PARISI DOMENICO , CECCHINO LIBERATO ROBERTO , CIANCIO FRANCESCO , INNOCENTI ALESSANDRO , PORTINCASA AURELIO 
Published: November 2020

Abstract
BACKGROUNDː The Epidermolysis Bullosa (EB) is a rare genetic disease associated with blistering skin caused by minimal trauma. Because of its exposed position and its continued use in daily activities, the hand is constantly at risk of microtrauma and is therefore one of the organs most affected by the disease with highly disabling deformities that represents a challenging field in hand surgery practice.METHODSː The Authors present their experience in the microsurgical treatment of pseudosyndactylies comparing the classic dressing with vaseline gauze with our innovative “glove protocol” using Integra® dermal regeneration template. The endpoints analyzed were: healing times, hospital stay time, discomfort for the patient, free-recurrence interval, follow-up range and major complications.RESULTSː A total of 34 procedures were performed on 24 EB patients with hand deformities. Compared with the dressing with vaseline gauze, microsurgery followed by application of dermal regeneration template gloves allowed a significant reduction of hospital stay, healing time, and dressing pain as well as an increased recurrence-free interval.CONCLUSIONSː The microsurgical approach followed by our new protocol described in the study has been beneficial in providing consistent and successful long-term results for these patients.
Evaluation of Pentosidine as Bone Quality Marker in Postmenopausal Breast Cancer Patients Receiving Aromatase Inhibitors
Authors: Shigematsu Hideo , Yoshiyama Tomoyuki , Yasui Daisuke , Ozaki Shinji 
Published: November 2020

Abstract
Background: Osteoporosis and fractures are important aromatase inhibitor (AI) related adverse events in postmenopausal women with hormone receptor positive breast cancer. An incremental increase of pentosidine is associated with a deterioration of bone quality. In this study, pentosidine was evaluated in postmenopausal breast cancer patients receiving AIs.Methods: Fifty Japanese postmenopausal breast cancer patients receiving AIs were retrospectively evaluated. Sixteen patients were given a bone modifying agent (BMA) concomitant with AIs. Changes of pentosidine, bone turnover markers and bone mineral density (BMD) before and after 12 months of AI therapy were compared between BMA administered patients (BMA group) and a non-BMA group. These factors were assessed by BMA groups using chi-square of categorical variables and t-test for continuous variables.Results: The median age of the subjects was 67 years, and 21, 23 and 6 subjects were classified as normal, bone loss and osteoporosis, respectively. There was no significant difference between pentosidine low and high groups in regard to age, height, weight, BMD of femoral neck and lumbar spine, and bone turnover markers including TRACP-5b and BAP. In the non-BMA group, pentosidine was increased in 18 cases (53%), and the average change of pentosidine was 21.5% (95%CI; 0.23 to 42.7%, p=0.048). In the BMA group, pentosidine was increased only in 2 cases (13%), and the average change of pentosidine was -16.6% (95%CI; -30.6 to -2.6%, p=0.023). There was a significantly lower proportion of pentosidine-increased cases (p=0.0065) and decrease of pentosidine (p=0.021) in the BMA group compared to those in the non-BMA group.Conclusions: Pentosidine was increased with AI, however, BMA inhibits an AI-induced increase of pentosidine in postmenopausal breast cancer patients.
Fetal sex and maternal postpartum depressive symptoms: findings from two prospective pregnancy cohorts
Authors: Cowell Whitney , Colicino Elena , Askowitz Talia , Nentin Farida , Wright Rosalind 
Published: November 2020

Abstract
Background: Fetal sex is known to modify the course and complications of pregnancy, with recent evidence of sex-differential fetal influences on the maternal immune and endocrine systems. In turn, heightened inflammation and surges in reproductive hormone levels associated with pregnancy and parturition have been linked with the development of perinatal depression. Here, we examined whether there is an association between fetal sex and maternal depression assessed during the prenatal and postnatal periods. Methods: The study included two multi-ethnic, prospective pregnancy cohorts that enrolled women from prenatal clinics in the Northeastern United States between 2001 and 2018. Maternal depressive symptoms were measured during the prenatal and postnatal periods using the Edinburgh Postpartum Depression Scale (EPDS) and newborn sex was reported by the mother following delivery. We used logistic regression to examine associations between fetal sex and maternal depressive symptoms (EPDS>10) during the prenatal period only, postnatal period only, or both periods versus no depressive symptoms during either period. We considered both unadjusted models and models adjusted for a core set of sociodemographic and lifestyle variables. Results: In adjusted models using PRISM data (N=528), women pregnant with a male versus female fetus had significantly greater odds of depressive symptoms during the postnatal period compared to women without depressive symptoms during either period (odds ratio [OR] = 5.24, 95% confidence interval [CI] = 1.93, 14.21). The direction of results was consistent in the ACCESS cohort, although the findings did not reach statistical significance (OR = 2.05, 95% CI = 0.86, 4.93). Significant associations were not observed in either cohort among women with prenatal symptoms only or women with prenatal and postnatal symptoms. Conclusions: Male fetal sex was associated with the onset of depressive symptoms during the postnatal period.
Screening of plant species for phytoremediation of synthetic textile dye wastewater
Authors: Kaur Navjeet , Kaushal Jyotsna , Mahajan Pooja , Srivas Arun Lal 
Published: November 2020

Abstract
Most of the dyes are carcinogenic and mutagenic in nature. Plants are potential candidates to remediate textile dye wastewater from contaminated sites. The present study aimed to screen potential plant species for removal of synthetic dye solution of triarylmethane dye Methylene Blue (MB) and diazo dye Congo Red (CR). The six plants selected for screening are Trachyspermum ammi (T. ammi), Tagetes erecta (T. erecta), Hibiscus rosa-sinensis (H. rosa- sinensis), Chrysanthemum indicum (C. indicum), Bryophyllum fedtschenkoi (B. fedtschenkoi), and Catharanthus roseus (C. roseus). The phytotreatment of dyes was done up to 40 h for two different concentrations of dyes i.e. 10 and 20 mg L−1. Among screened plant species, the maximum decolorization was obtained from T. ammi followed by B. fedtschenkoi. Both of these plant species showed active growth even after the phytoremediation process. T. ammi decolorized the MB dye 99% (10 mg L−1) and 86% (20 mg L−1) while the decolorization of the CR dye solution was up to 95% (10 mg L−1) and 84% (20 mg L−1). T. ammi found to have maximum potential among screened plants for the removal of MB and CR dye from synthetic dye solution and can be used for phytoremediation of wastewater contaminated with synthetic dyes.
Influence of flow rate, fluid temperature, and extension line on Hotline and S-line heating capability: an in vitro study
Authors: Kim Hosu , Kim Tae Kyong , Yoo Sukha , Kim Jin-Tae 
Published: November 2020

Abstract
Background A fluid warmer can prevent hypothermia during the perioperative period. This study evaluated the heating capabilities of Hotline and Barkey S-line under different flow rates and initial fluid temperatures, as well as after the extension line installation. Methods We measured the temperature of a 0.9% sodium chloride solution at the fluid warmer outlet (TProx) and the extension line end (TDistal) with three different initial fluid temperatures (room, warm, and cold) and two flow rates (250 ml/hr and 100 mL/hr). Results At a 250 ml/hr flow rate, the TProx and TDistal values were observed to be higher in Hotline than in S-line when using room-temperature or cold fluid. Administering of the warm fluid at the same flow rate significantly increased the TProx and TDistal values in S-line more than the cold and room-temperature fluids. At flow rates of 100 ml/hr, TDistal values were significantly lower than TProx values in both devices regardless of the initial fluid temperature. Conclusions Hotline outperformed S-line for warming fluids at a high flow rate with cold or room-temperature fluids. Administering warm fluid in S-line prevented a decrease in the fluid temperature at a high flow rate. However, at a low flow rate, the fluid temperature significantly decreased in both devices after passing through an extension line.
Sex&nbsp;Differences in Gut Microbiome&nbsp;in&nbsp;High-Fat-Diet Fed&nbsp;Rats
Authors: Shi Ying , Yue Fangzhi , Xing Lin , Wu Shanyu , Wei Lin , Xiang Xingwei , Russell Ryan , Xia Ke , Zhang Dongmei 
Published: November 2020

Abstract
Background: Sex differences in obesity and related metabolic diseases are well recognized, however, the mechanism has not been elucidated. Gut microbiota and its metabolites may play a vital role in the development of obesity and metabolic diseases. The aim of the present study was to investigate sex differences in gut microbiota and its metabolites in a high-fat-diet (HFD) obesity rats and identify microbiota genera potentially contributing to such differences in obesity and non-alcoholic fatty liver disease (NAFLD) susceptibility.Methods: Sprague–Dawley rats were divided into four groups (eight animals per group): (1) male rats on a normal diet (MND), (2) male rats on HFD (MHFD), (3) female rats on a normal diet (FND), and (4) female rats on HFD (FHFD). Body weight, liver pathology, gut microbiota and short/medium chain fatty acids in colon contents were compared between different sexes.Results: HFD induced more body weight gain and fat storage in female rats, however, lower hepatic steatosis in FHFD than in MHFD rats was observed. When considering gut microbiota composition, FHFD rats had lower microbiome diversity than MHFD. A significant increase of Firmicutes phylum, along with Bilophila and Blautia genus was detected in MHFD rats, as compared with FHFD, which showed increased relative abundance of Murimonas. Moreover, propionic and lauric acid levels were higher in FHFD than those in MHFD rats. Conclusions: HFD induced sex-related alterations in gut microbiome and fatty acids. Furthermore, the genus Bilophila, Blautia and Murimonas might contribute to sex differences observed in obesity and NAFLD susceptibility.
Numerical simulation research on steel/ceramic composite target plate structure against penetration of high-speed projectile
Authors: Wu Gang , Wang Xin , Lu Xiaofei , Ji Chong , Zhao Changxiao , Zhu Haojie 
Published: November 2020

This study is mainly based on LS-DYNA to simulate the entire process of high-speed projectile penetration into the steel-ceramic composite target structure. In the process of projectile penetration into the target, the impact speed of projectile, the structure of steel-ceramic composite target plate and other key factors on the target failure and target transient response and the law are explored. The article tried different thickness combinations of the steel plate ceramic structure, obtained the best thickness combination according to the after-effect of penetration, and analyzed the remaining velocity and remaining rod length of the projectile. The results show that the composite target plate has the best protection performance under the condition of the thickness ratio of panel-sandwich-back plate of 1:2:1, and the composite target plate has better anti-penetration performance against projectiles with a speed greater than 1500 m/s.