bluuebunny/crossref_metadata_2025_split huggingface dataset:
Using Analysis Corrections to Address Model Error in Atmospheric Forecasts
Authors: Crawford William , Frolov Sergey , McLay Justin , Reynolds Carolyn A. , Barton Neil , Ruston Benjamin , Bishop Craig H. 
Published: August 2020

Abstract
This paper illustrates that analysis corrections, when applied as a model tendency term, can be used to improve nonlinear model forecasts and are consistent with the hypothesis that they represent an additive 6-h accumulation of model error. Comparison of mean analysis corrections with observational estimates of bias further illustrates the fidelity with which mean analysis corrections capture the model bias. While most previous implementations have explored the use of analysis corrections to correct forecast biases in short-range forecasts, this is the first implementation of the correction method using both a seasonal mean and random analysis correction for medium-range forecasts (out to 10 days). Overall, the analysis correction–based perturbations are able to improve forecast skill in ensemble and deterministic systems, especially in the first 5 days of the forecast where bias and RMSE in both lower-tropospheric temperature and 500 hPa geopotential height are significantly improved across all experiments. However, while the method does provide some significant improvement to forecast skill, some degradation in bias can occur at later lead times when the average bias at analysis time trends toward zero over the length of the forecast, leading to an overcorrection by the analysis correction–based additive inflation (ACAI) method. Additionally, it is shown that both the mean and random component of the ACAI perturbations play a role in adjusting the model bias, and that the two components can have a complicated and sometimes nonlinear interaction.
Impacts of Predicting the Liquid Fraction of Mixed-Phase Particles on the Simulation of an Extreme Freezing Rain Event: The 1998 North American Ice Storm
Authors: Cholette Mélissa , Thériault Julie M. , Milbrandt Jason A. , Morrison Hugh 
Published: August 2020

Abstract
A prognostic equation for the liquid fraction of mixed-phase particles has been recently added to the Predicted Particle Properties (P3) bulk microphysics scheme. Mixed-phase particles are necessary to simulate key microphysical processes leading to various winter precipitation types, such as ice pellets and freezing rain. To illustrate the impacts of predicting the bulk liquid fraction, the 1998 North American Ice Storm is simulated using the Weather Research and Forecasting (WRF) Model with the modified P3 scheme. It is found that simulating partial melting by predicting the bulk liquid fraction produces higher mass and number mixing ratios of rain. This leads to smaller rain sizes reaching the refreezing layer as well as a decrease in the freezing rain accumulation at the surface by up to 30% in some locations compared to when no liquid fraction is predicted. The increase in fall speed and density and decrease of particle diameter during partial melting combined with an improved representation of the refreezing process in the modified P3 leads to generally higher total solid surface precipitation rates than using the original P3 scheme. There is also an increase of solid precipitation in regions of ice pellet accumulation. Overall, the simulation of mixed-phase particles notably impacts the vertical and spatial distributions of precipitation properties.
An Implicit–Explicit Vertical Transport Scheme for Convection-Allowing Models
Authors: Wicker Louis J. , Skamarock William C. 
Published: August 2020

Abstract
An adaptive implicit–explicit vertical transport method is implemented in the Advanced Research version of the Weather Research and Forecasting Model (WRF-ARW), and improved integration efficiency is demonstrated for configurations employing convective-allowing horizontal and vertical resolutions. During the warm season over the continental United States, stable forecasts at convective-allowing resolutions are more challenging because localized regions of extreme thermodynamic instability generate large vertical velocities within thunderstorms that cause the integrations to become unstable because of violations of the Courant–Friedrichs–Lewy (CFL) condition for the explicit advection scheme used in WRF-ARW. The implicit–explicit vertical transport scheme removes the CFL instability but maintains accuracy for typical vertical velocities. Tests using this scheme show that the new scheme permits a time step that is 20%–25% percent larger, and it reduces the wall clock time by 10%–13% percent relative to a configuration similar to a current operational convection-allowing model while also producing more realistic updraft intensities within the most intense storms. Other approaches to maintain stability are either less efficient (e.g., reducing the time step) or significantly impact the solution accuracy (e.g., increasing the damping and/or reducing the latent heating, which severely limits the updraft magnitudes during the forecasts).
SWRL Net: A Spectral, Residual Deep Learning Model for Improving Short-Term Wave Forecasts
Authors: Mooneyham Jonny , Crosby Sean C. , Kumar Nirnimesh , Hutchinson Brian 
Published: August 2020

AbstractSkillful nearshore wave forecasts are critical for providing timely alerts of hazardous wave events that impact navigation or recreational beach use. While typical forecasts provide bulk wave parameters (wave height and period), spectral details are needed to correctly predict wave and associated circulation dynamics in the nearshore region. Currently, global wave models, such as WAVEWATCH III (WW3), make spectral predictions, but do not assimilate regional buoy observations. Here, Spectral Wave Residual Learning Network (SWRL Net), a fully convolutional neural network, is trained to take recent WW3 forecasts and buoy observations, and produce corrections to frequency-directional WW3 spectra, transformed into directional buoy moments, for up to 24 h in the future. SWRL Net is trained with 10 years of collocated NOAA’s WW3 CFSR reanalysis predictions and buoy observations at three locations offshore of the U.S. western coast. At buoy locations SWRL Net residual corrections result in wave height root-mean-square error (RMSE) reductions of 23%–50% in the first 6 h and 10%–20% thereafter. Sea frequencies (5–10 s) show the most improvement compared to swell (12–20 s). SWRL Net reduces mean direction RMSE by 28%–54% and mean period RMSE by 20%–56% over 24 forecast hours. While each model is trained and tested at independent locations, SWRL Net exhibits generalization when introduced to data from other locations, suggesting future development may be composed of training sets from multiple locations.
The effect of minimum wages on consumption in Canada
Authors: Jung Young Cheol , McFarlane Adian , Das Anupam 
Published: August 2020

We use Canadian data over the period of 1991Q1 to 2019Q2 to examine the effect of higher minimum wages on consumption, measured as the real retail trade sales per adult population. Such an examination is rare in the extant literature and it is timely given the increasing debate concerning the stimulus versus inflationary effects arising from wage polices because of COVID-19 global pandemic. We apply the autoregressive distributed lag model to determine the causal relationship between these variables. We find one long-run cointegrating relationship that runs from the real minimum wage to the real retail trade sales. In addition, we find that a 1% increase in the minimum wage is associated with almost a 0.5% increase in real retail trade sales in the long run. While our findings rest on several statistical assumptions, there is strong evidence in support of the position that minimum wage strengthens aggregate consumer spending, and thereby the standard of living, economic growth and stability. This is a position that differs from the conclusions drawn from mainstream academic and policy debates on the economic usefulness and efficacy of minimum wage increases. JEL Codes: C30, E21, E24
Healthy Intimate Relationships and the Adult With Autism
Authors: Girardi Amanda , Curran Mary Sharon , Snyder Briana L. 
Published: August 2020

Objective: An adult faces many challenges; however, one experience that is often taken for granted by neurotypical individuals is building intimate relationships. The purpose of this article is to discuss the barriers for healthy intimate relationships for an individual with autism, strategies to overcome these barriers, recommendations for future practice and research, and resources to improve knowledge of this important yet overlooked topic. Method: Data collection was done using research journals and databases such as EBSCO, SAGE Knowledge, MEDLINE, and CINAHL. The reviewed articles were published from 2015 to the present time. During the search process, the following key phrases were used: autism spectrum disorder, intimate relationships, sexuality education, and social skills. In total, 1,400 articles were found with an additional 52 sources found through other sources, of which 31 met inclusion criteria and were subsequently reviewed. Results: Although those with autism have many strengths and talents, deficits in communication and social interaction can affect the formation of healthy adult relationships, including friendships and romantic relationships. In addition to social challenges, a lack of education regarding safe and healthy relationships can put an individual with autism at risk. Conclusions: It is imperative that that individuals with autism, their families, and health care professionals are educated about the barriers and able to access educational resources and specialized curricula on this topic.
Spider phylosymbiosis: divergence of widow spider species and their tissues’ microbiomes
Authors: Dunaj Sara J. , Bettencourt Brian R. , Garb Jessica E. , Brucker Robert M. 
Published: August 2020

Abstract
Background
Microbiomes can have profound impacts on host biology and evolution, but to date, remain vastly understudied in spiders despite their unique and diverse predatory adaptations. This study evaluates closely related species of spiders and their host-microbe relationships in the context of phylosymbiosis, an eco-evolutionary pattern where the microbial community profile parallels the phylogeny of closely related host species. Using 16S rRNA gene amplicon sequencing, we characterized the microbiomes of five species with known phylogenetic relationships from the family Theridiidae, including multiple closely related widow spiders (L. hesperus, L. mactans, L. geometricus, S. grossa, and P. tepidariorum).

Results
We compared whole animal and tissue-specific microbiomes (cephalothorax, fat bodies, venom glands, silk glands, and ovary) in the five species to better understand the relationship between spiders and their microbial symbionts. This showed a strong congruence of the microbiome beta-diversity of the whole spiders, cephalothorax, venom glands, and silk glands when compared to their host phylogeny. Our results support phylosymbiosis in these species and across their specialized tissues. The ovary tissue microbial dendrograms also parallel the widow phylogeny, suggesting vertical transfer of species-specific bacterial symbionts. By cross-validating with RNA sequencing data obtained from the venom glands, silk glands and ovaries of L. hesperus, L. geometricus, S. grossa, and P. tepidariorum we confirmed that several microbial symbionts of interest are viably active in the host.

Conclusion
Together these results provide evidence that supports the importance of host-microbe interactions and the significant role microbial communities may play in the evolution and adaptation of their hosts.
The aceE involves in mycolic acid synthesis and biofilm formation in Mycobacterium smegmatis
Authors: Chen Suting , Teng Tianlu , Wen Shuan , Zhang Tingting , Huang Hairong 
Published: August 2020

Abstract
Background
The integrity of cell wall structure is highly significant for the in vivo survival of mycobacteria. We hypothesized that changes in morphology may indicate changes in cell wall metabolism and identified an aceE gene mutant (aceE-mut) which presented a deficient colony morphology on 7H10 agar by screening transposon mutagenesis in Mycolicibacterium smegmatis, basonym Mycobacterium smegmatis (M. smegmatis). This study aimed to identify the functional role of aceE gene in cell wall biosynthesis in M. smegmatis.

Results
We observed that the colony morphology of aceE-mut was quite different, smaller and smoother on the solid culture medium than the wild-type (WT) strain during the transposon library screening of M. smegmatis. Notably, in contrast with the WT, which aggregates and forms biofilm, the aceE-mut lost its ability of growing aggregately and biofilm formation, which are two very important features of mycobacteria. The morphological changes in the aceE-mut strain were further confirmed by electron microscopy which indicated smoother and thinner cell envelope images in contrast with the rough morphology of WT strains. Additionally, the aceE-mut was more fragile to acidic stress and exhibited a pronounced defects in entering the macrophages as compared to the WT. The analysis of mycolic acid (MA) using LC-MS indicated deficiency of alpha-MA and epoxy-MA in aceE-mut strain whereas complementation of the aceE-mut with a wild-type aceE gene restored the composition of MA.

Conclusions
Over all, this study indicates that aceE gene plays a significant role in the mycolic acid synthesis and affects the colony morphology, biofilm formation of M. smegmatis and bacteria invasion of macrophage.
AtPIG-S, a predicted Glycosylphosphatidylinositol Transamidase subunit, is critical for pollen tube growth in Arabidopsis
Authors: Desnoyer Nicholas , Howard Gregory , Jong Emma , Palanivelu Ravishankar 
Published: August 2020

Abstract
Background
Glycosylphosphatidylinositol (GPI) addition is one of the several post-translational modifications to proteins that increase their affinity for membranes. In eukaryotes, the GPI transamidase complex (GPI-T) catalyzes the attachment of pre-assembled GPI anchors to GPI-anchored proteins (GAPs) through a transamidation reaction. A mutation in AtGPI8 (gpi8–2), the putative catalytic subunit of GPI-T in Arabidopsis, is transmitted normally through the female gametophyte (FG), indicating the FG tolerates loss of GPI transamidation. In contrast, gpi8–2 almost completely abolishes male gametophyte (MG) function. Still, the unexpected finding that gpi8–2 FGs function normally requires further investigation. Additionally, specific developmental defects in the MG caused by loss of GPI transamidation remain poorly characterized.

Results
Here we investigated the effect of loss of AtPIG-S, another GPI-T subunit, in both gametophytes. Like gpi8–2, we showed that a mutation in AtPIG-S (pigs-1) disrupted synergid localization of LORELEI (LRE), a putative GAP critical for pollen tube reception by the FG. Still, pigs-1 is transmitted normally through the FG. Conversely, pigs-1 severely impaired male gametophyte (MG) function during pollen tube emergence and growth in the pistil. A pPIGS:GFP-PIGS transgene complemented these MG defects and enabled generation of pigs-1/pigs-1 seedlings. However, the pPIGS:GFP-PIGS transgene seemingly failed to rescue the function of AtPIG-S in the sporophyte, as pigs-1/pigs-1, pPIGS:GFP-PIGS seedlings died soon after germination.

Conclusions
Characterization of pigs-1 provided further evidence that the FG tolerates loss of GPI transamidation more than the MG and that the MG compared to the FG may be a better haploid system to study the role of GPI-anchoring. Pigs-1 pollen develops normally and thus represent a tool in which GPI anchor biosynthesis and transamidation of GAPs have been uncoupled, offering a potential way to study free GPI in plant development. While previously reported male fertility defects of GPI biosynthesis mutants could have been due either to loss of GPI or GAPs lacking the GPI anchor, our results clarified that the loss of mature GAPs underlie male fertility defects of GPI-deficient pollen grains, as pigs-1 is defective only in the downstream transamidation step.
Safety of intraoperative hypothermia for patients: meta-analyses of randomized controlled trials and observational studies
Authors: Xu He , Wang Zijing , Guan Xin , Lu Yijuan , Malone Daniel Charles , Salmon Jack Warren , Ma Aixia , Tang Wenxi 
Published: August 2020

AbstractBackgroundPrevious studies have shown that intraoperative hypothermia was associated with higher risks of clinical adverse events, but we found otherwise from recent evidences. This study aims to synthesize the existing evidence evaluating safety of intraoperative hypothermia.MethodsArticles, reviews, ongoing trials and grey literatures were retrieved from PubMed, The Cochrane Library, Clinical Trails and CNKI (a Chinese national database) till February 2nd, 2019. Both randomized controlled trials and observational studies compared incidences of all sorts of intra- and post-operative consequences between hypothermia and normothermia were included. Researches comparing different warming systems were excluded. We also examined risks of hypothermia using lowered standards (35.5 °C and 35 °C) from a Chinese trial (ChiCTR-IPR-17011099).ResultsA total of 9 RCT studies and 11 observational studies were included. RCT-synthesized results showed that intraoperative hypothermia was associated with higher risks of bleeding (MD = 131.90, 95%CI: 117.42, 146.38), surgical site infection (RD = 0.14, 95%CI: 0.06, 0.21) and shivering (RD = 0.32, 95%CI: 0.06, 0.58) but with no significant differences in duration of surgery, hospital stay or mortality. Observational study-synthesized evidences showed that intraoperative hypothermia did not result in higher risks in any of these adverse events. Results didn’t change even if the standard of hypothermia was lowered by 0.5–1.0 °C.ConclusionsThe study indicates that the synthesized risks resulted by intra-operative hypothermia might be overestimated and the eligibility of 36 °C to define hypothermia is not sensitive enough. Given body-temperature protection has not been popularized in China, it is still critical to normalize the hypothermia prevention at this stage.
The relationship between preoperative T helper cytokines in the ileal mucosa and the pathogenesis of pouchitis
Authors: Kitajima Takahito , Okita Yoshiki , Kawamura Mikio , Kondo Satoru , Toiyama Yuji , Uchida Keiichi , Kusunoki Masato 
Published: August 2020

AbstractBackgroundAlthough the etiology of pouchitis remains unknown, inflammatory cytokines are significantly associated with the pathogenesis of pouchitis. The cytokine responses that characterize inflammatory bowel diseases (IBD) are key pathogenic components of the disease. Although cytokine profiles in the colonic mucosa have been investigated in experimental colitis models or IBD patients, cytokine profiles in the ileal mucosa at colectomy have been rarely assessed.AimTo assess the relationship between pouchitis and T helper (Th) cytokines in the ileal mucosa collected at the time of colectomy and pouch construction.MethodsThis retrospective study involved 68 consecutive patients from January 2004 to May 2011 who underwent ileal pouch–anal anastomosis for ulcerative colitis. Samples were obtained from the terminal ileum of resected specimens at time of total colectomy or subtotal colectomy. mRNA expression levels of Th cytokines (IFN-γ, IL-23A, IL-5, IL-13 and IL-17A) were determined.ResultsForty of 68 patients (58.8%) developed pouchitis. There was no association between IL-23A expression levels and incidence of pouchitis (p = 0.301). Patients with elevated IFN-γ had a significantly higher incidence of pouchitis compared with low IFN-γ patients (p = 0.043). Univariate analysis demonstrated a total dose of prednisolone > 7000 mg administered before colectomy (p = 0.04) and high IFN-γ expression (p = 0.02) were significant risk factors for pouchitis onset. In multivariate analysis, elevated IFN-γ messenger(m)RNA levels were significantly associated with pouchitis onset (p = 0.03).ConclusionIFN-γ expression in the normal ileal mucosa at the time of colectomy may be an important factor in the pathophysiology of pouchitis.
Rhino-orbital mucormycosis due to Apophysomyces ossiformis in a patient with diabetes mellitus: a case report
Authors: Martínez-Herrera Erick , Frías-De-León María Guadalupe , Julián-Castrejón Angélica , Cruz-Benítez Luis , Xicohtencatl-Cortes Juan , Hernández-Castro Rigoberto 
Published: August 2020

Abstract
Background
The most common aetiological agents of mucormycosis are Rhizopus, Mucor, Apophysomyces and Lichtheimia. Apophysomyces is comparatively rare, as it has been reported in less than 3% of mucormycosis cases. The genus Apophysomyces includes six species, and only A. elegans, A. mexicanus, A. variabilis and A. ossiformis have been reported to cause infections in both immunocompetent and immunocompromised patients.

Case presentation
We present a case of a 46-year-old male patient with bilateral blepharoedema, corneal opacity in the left eye and poorly controlled diabetes mellitus. The patient was subjected to total maxillectomy, exenteration of the left orbit and treatment with liposomal amphotericin B. Direct mycological analysis with KOH 10% revealed hyaline, coenocytic, long and wide hyphae. Apophysomyces ossiformis was identified from maxillary biopsy using 18S-ITS1–5.8S-ITS2-28S rRNA gene amplification and sequencing. The patient requested to be transferred to another hospital to continue treatment, where he died on the ninth day after admittance.

Conclusion
To the best of our knowledge, this is the first case of rhino-orbital mucormycosis due to A. ossiformis with a fatal outcome. This case reveals the need to identify the fungus causing mucormycosis with molecular methods to identify adequate treatment therapies for patients with this infection.