bluuebunny/crossref_metadata_2025_split huggingface dataset:
Kidney transplantation from a living donor with renal artery fibromuscular dysplasia: A case report on arterial grafting of the donor renal artery
Authors: Matsushita Yuto , Motoyama Daisuke , Ito Toshiki , Sugiyama Takayuki , Otsuka Atsushi , Sano Masaki , Inuzuka Kazunori , Miyake Hideaki 
Published: July 2020

IntroductionRenal artery fibromuscular dysplasia is generally considered a contraindication of kidney transplantation, since fibromuscular dysplasia occasionally induces hypertension or renal insufficiency in the recipient and/or donor. To date, limited information remains available with respect to whether kidneys with renal arterial fibromuscular dysplasia can be successfully transplanted.Case presentationA 53‐year‐old potential donor was diagnosed with fibromuscular dysplasia of the right renal artery. Laparoscopic right nephrectomy was performed, and the affected portion was resected and reconstructed using the harvested internal iliac artery. Transplantation was successful and the serum creatinine level was <2 mg/dL for 3 years after surgery.ConclusionIf reconstruction of the diseased artery could be safely performed, transplantation using a kidney from a donor with renal artery fibromuscular dysplasia may be considered.
Foam‐in‐vein: A review of rheological properties and characterization methods for optimization of sclerosing foams
Authors: Meghdadi Alireza , Jones Stephen A. , Patel Venisha A. , Lewis Andrew L. , Millar Timothy M. , Carugo Dario 
Published: July 2020

AbstractVaricose veins are chronic venous defects that affect >20% of the population in developed countries. Among potential treatments, sclerotherapy is one of the most commonly used. It involves endovenous injection of a surfactant solution (or foam) in varicose veins, inducing damage to the endothelial layer and subsequent vessel sclerosis. Treatments have proven to be effective in the short‐term, however recurrence is reported at rates of up to 64% 5‐year post‐treatment. Thus, once diagnosed with varicosities there is a high probability of a permanently reduced quality of life. Recently, foam sclerotherapy has become increasingly popular over its liquid counterpart, since foams can treat larger and longer varicosities more effectively, they can be imaged using ultrasound, and require lower amounts of sclerosing agent. In order to minimize recurrence rates however, an investigation of current treatment methods should lead to more effective and long‐lasting effects. The literature is populated with studies aimed at characterizing the fundamental physics of aqueous foams; nevertheless, there is a significant need for appropriate product development platforms. Despite successfully capturing the microstructural evolution of aqueous foams, the complexity of current models renders them inadequate for pharmaceutical development. This review article will focus on the physics of foams and the attempts at optimizing them for sclerotherapy. This takes the form of a discussion of the most recent numerical and experimental models, as well as an overview of clinically relevant parameters. This holistic approach could contribute to better foam characterization methods that patients may eventually derive long term benefit from.
Potential clinical application of an automated fluorescent microbial cell counter in the detection of urinary tract infection
Authors: Phillips Laila E. , Verma Sandeep , Surapaneni Balarama K. , Dutta Sudhir K. 
Published: July 2020

AbstractBackgroundUrinary tract infections (UTI) account for millions of office visits and approximately 400 000 hospital admissions every year in the United States; as a result, the cost burden of UTI in the USA is estimated at approximately $2.8 billion. There is a great deal of interest in finding newer, faster, and more reliable methods for diagnosing UTI as compared to the standard urine culture. MethodsAn automated fluorescent microbial cell counter was used to compare urine samples found to be positive for Escherichia coli UTI via cell culturing (n = 11) with UTI‐negative samples (n = 10).ResultsPatients with a positive urine culture had significantly higher cell count results using the microbial cell counter (1.01 × 108 cells/mL) as compared to the negative samples (2.35 × 106 cells/mL; P = .0022).ConclusionsThese observations suggest that automated microbial cell counters may serve as a rapid, objective method for the detection of bacteriuria in urine samples submitted for evaluation of suspected UTI.
CTLA‐4 and HLA‐DQ are key molecules in the regulation of mDC‐mediated cellular immunity by Tregs in severe aplastic anemia
Authors: Liu Bingnan , Shao Yuanyuan , Liang Xiaowei , Lu Dan , Yan Li , Churov Alexey , Fu Rong 
Published: July 2020

AbstractBackgroundRegulatory T cells (Tregs) inhibit the activation of cluster of differentiation (CD) 4+, CD8+T cells and the antigen‐presenting process of antigen‐presenting cells, and may play an important role in acquired severe aplastic anemia (SAA).MethodsFlow cytometry was used to measure CD4+CD25+CD127dim Tregs, cytotoxic T lymphocyte antigen 4 (CTLA‐4) expression on Tregs, and human leukocyte antigen (HLA)‐DQ expression on myeloid dendritic cells (mDCs). The correlations of CTLA‐4 and HLA‐DQ with immune status and clinical indicators and the changes in these indicators after immunosuppressive therapy (IST) were analyzed.ResultsIn SAA patients, the number of Tregs and their CTLA‐4 expression were low but recovered after IST; the HLA‐DQ expression on mDCs was high but decreased after IST. The CTLA‐4 expression on Tregs and the HLA‐DQ expression on mDCs showed a negative correlation. The CTLA‐4 on Tregs was positively but HLA‐DQ on mDCs negatively correlated with the number of Tregs, natural killer (NK) cell number, and CD4+T/CD8+T ratio. CTLA‐4 was positively but HLA‐DQ negatively correlated with the percentage of granulocytoid and erythroid cells in bone marrow, white blood cell count in PB, absolute neutrophil count in PB, and the percentage of reticulocytes in PB.ConclusionsCTLA‐4/HLA‐DQ may be key in the regulation of Tregs on mDCs in SAA patients. Our findings should be helpful for further investigation of the mechanism of immune pathogenesis in SAA patients. Studies on the regulators of Treg and CTLA‐4 activity will be valuable for SAA therapeutic target research and disease monitoring.
LINC00319 promotes osteosarcoma progression by regulating the miR‐455‐3p/NFIB axis
Authors: Sun Farui , Yu Ziliang , Wu Bingbing , Zhang Haiping , Ruan Jing 
Published: July 2020

AbstractBackgroundNumerous studies have shown that aberrant expression of long non‐coding RNAs (lncRNAs) is associated with the development and metastasis of osteosarcoma (OS). However, the role and function of LINC00319 with respect to regulating OS progression is unknown. The present study aimed to reveal the function and related mechanism of LINC00319 in OS.MethodsThe expression of LINC00319, miR‐455‐3p and nuclear factor IB (NFIB) in OS cells and tissues was determined using a reverse transcriptase‐polymerase chain reaction (PCR). The sublocalization of LINC00319 was predicted by the lncATLAS database (http://lncatlas.crg.eu) and RNA fluorescence in situ hybridization (FISH) was further performed to detect the subcellular localization of LINC00319. LINC00319, miR‐455‐3p and NFIB target sites were predicted by StarBase (http://starbase.sysu.edu.cn/index.php) and validated using a dual luciferase reporter gene assay. We subsequently performed LINC00319 gain‐ and loss‐of‐function studies to define the role of LINC00319 in OS cell migration.ResultsPCR results showed that lncRNA LINC00319 exhibited high expression in tumor cells and tissue. Moreover, LINC00319 was positioned in the cytoplasm, which was identified by FISH. Knockdown of lncRNA LINC00319/NFIB or overexpression of miR‐455‐3p blocked the migration of OS cells. In addition, the inhibitory effect of migration with the knockdown of lncRNA LINC00319 was partially blocked by administration of miR‐455‐3p inhibitor.ConclusionslncRNA LINC00319 may promote OS progression by regulating the miR‐455‐3p/NFIB axis, which probably serves as an innovative potential indicator of prognosis and a target of therapy for OS.
Risk factors for pancreatic fistula grade C after pancreatoduodenectomy: A large prospective, multicenter Japan‐Taiwan collaboration study
Authors: Hirono Seiko , Shimokawa Toshio , Nagakawa Yuichi , Shyr Yi‐Ming , Kawai Manabu , Matsumoto Ippei , Satoi Sohei , Yoshitomi Hideyuki , Okabayashi Takehiro , Motoi Fuyuhiko , Amano Ryosuke , Murakami Yoshiaki , Hirano Satoshi , Kawamoto Kazuyuki , Nakamori Shoji , Shan Yan‐Shen , Kobayashi Shinjiro , Nitta Hiroyuki , Matsukawa Hiroyoshi , Uchiyama Kazuhisa , Hsu Chih‐Po , Kitami Chie , Yamamoto Masakazu , Hwang Tsann‐Long , Yamaue Hiroki 
Published: July 2020

AbstractBackground/PurposeGrade C postoperative pancreatic fistula (POPF), as defined by International Study Group of Pancreatic Fistula (ISGPF), is the most life‐threatening complication after pancreatoduodenectomy (PD). This study aims to evaluate risk factors for Grade C POPF after PD.MethodsThis is a prospective, multicenter study based in Japan and Taiwan. Between December 2014 and May 2017, 3022 patients were enrolled in this study and 2762 patients were analyzed. We analyzed risk factors of Grade C POPF based on the updated 2016 ISGPF scheme (organ failure, reoperation, and/or death).ResultsAmong 2762 patients, 46 patients (1.7%) developed Grade C POPF after PD. The mortality rate of the 46 patients with Grade C POPF was 37.0%. On the multivariate analysis, six independent risk factors for Grade C POPF were found; BMI ≥ 25.0 kg/m2, chronic steroid use, preoperative serum albumin <3.0 mg/dL, soft pancreas, operative time ≥480 minutes, and intraoperative transfusion. The c‐statistic of our risk scoring model for Grade C POPF using these risk factors was 0.77. The score was significantly higher in Grade C POPF than in Grade B POPF (P < .001) or none/biochemical leak (P < .001).ConclusionsThis prospective study showed risk factors for Grade C POPF after PD.
First synthesis of thiazepino[3,4‐a]isoquinolines, a facile new synthetic route to diazepino[3,4‐a]isoquinolines and assessment of their dopamine and σ receptor affinities
Authors: Cordone Pierpaolo , Namballa Hari Krishna , Harding Wayne Wesley 
Published: July 2020

AbstractHeterocycles that bear the novel 5,6,14,14a‐tetrahydro‐8H‐benzo[6,7][1,4]thiazepino[3,4‐a]isoquinoline and the 5,6,14,14a‐tetrahydro‐8H‐13l2‐benzo[6,7][1,4]diazepino[3,4‐a]isoquinoline frameworks were synthesized in a facile manner. These tetrahydroprotoberberine (THPB)‐inspired scaffolds demonstrate selective affinity for the σ1R in contrast to the naturally occurring THPB congeners that show D1R and σ2R selectivity.
Synthesis of novel spiro‐fused pyrazolo[4′,3′:5,6]pyrido[2,3‐d]pyrimidines
Authors: Fazlelahi Hadi Z. , Moghadam Peyman N. , Baradarani Mehdi M. , Joule John A. 
Published: July 2020

AbstractNew pyrazolo[4′,3′:5,6]pyrido[2,3‐d]pyrimidines, with an associated spiro‐3,3′‐oxindole attachment, were prepared by three‐component combinations of 5,6‐dihydro‐4H‐pyrrolo[3,2,1‐ij]quinoline‐1,2‐dione with a pair of reactants chosen from a pyrazol‐5‐one, a pyrazole‐5‐amine, a barbituric acid, or a 6‐aminouracil.
Multicultural Education and Perceived Multicultural Competency of School Counselors
Authors: Dameron Merry Leigh , Camp Ami , Friedmann Beatriz , Parikh‐Foxx Sejal 
Published: July 2020

A national survey of practicing school counselors (N= 755) revealed significant differences between participants’ perceived multicultural competence based on the type of multicultural training received. Participants who took infused multicultural coursework had significantly higher mean scores in multicultural awareness, knowledge, and skills than participants who reported taking a single multicultural course.Una encuesta nacional de consejeros escolares en activo (N= 755) reveló diferencias significativas en la competencia multicultural percibida de los participantes según el tipo de capacitación multicultural recibida. Los participantes que tomaron cursos que incluían contenidos multiculturales obtuvieron puntajes medios significativamente más altos en consciencia, conocimientos y habilidades multiculturales que aquellos participantes que indicaron haber tomado un único curso multicultural.
Measuring Multicultural Training Reactance: Initial Instrument Psychometrics
Authors: Lowery Robyn Crowell , Borders L. DiAnne , Ackerman Terry A. 
Published: July 2020

Resistant behaviors in multicultural courses are a common dilemma within counselor education. On the basis of psychological reactance theory (J. W. Brehm, 1966), the authors created a multicultural training reactance scale to measure the construct. Exploratory factor analysis and initial reliability and validity data are presented, followed by suggestions that address course reactions.Los comportamientos resistentes en los cursos multiculturales son un dilema frecuente en la educación de consejeros. Basándose en la teoría de reactancia psicológica (J. W. Brehm, 1966), los autores crearon una escala de reactancia en la capacitación multicultural para medir el constructo. Se presenta un análisis factorial exploratorio, así como datos iniciales de fiabilidad y validez, seguidos por sugerencias que abordan las reacciones al curso.
Benign Neoplasms, Mass‐Like Infections, and Pseudotumors That Mimic Hepatic Malignancy at MRI
Authors: Costa Andreu F. , Clarke Sharon E. , Stueck Ashley E. , McInnes Matthew D.F. , Thipphavong Seng 
Published: July 2020

A variety of conditions may mimic hepatic malignancy at MRI. These include benign hepatic tumors and tumor‐like entities such as focal nodular hyperplasia‐like lesions, hepatocellular adenoma, hepatic infections, inflammatory pseudotumor, vascular entities, and in the cirrhotic liver, confluent fibrosis, and hypertrophic pseudomass. These conditions demonstrate MRI features that overlap with hepatic malignancy, and can be challenging for radiologists to diagnose accurately. In this review we discuss the MRI manifestations of various conditions that mimic hepatic malignancy, and highlight features that may allow distinction from malignancy.Level of Evidence5Technical Efficacy Stage3
Preliminary Assessment of Intravoxel Incoherent Motion Diffusion‐Weighted MRI (IVIM‐DWI) Metrics in Alzheimer's Disease
Authors: Bergamino Maurizio , Nespodzany Ashley , Baxter Leslie C. , Burke Anna , Caselli Richard J. , Sabbagh Marwan N. , Walsh Ryan R. , Stokes Ashley M. 
Published: July 2020

BackgroundAlzheimer's disease (AD) is a progressive neurodegenerative disease that affects aging populations. Current MRI techniques are often limited in their sensitivity to underlying neuropathological changes.PurposeTo characterize differences in voxel‐based morphometry (VBM), apparent diffusion coefficient (ADC), and intravoxel incoherent motion diffusion‐weighted imaging (IVIM‐DWI) metrics in aging populations. Additionally, to investigate the connection between cognitive assessments and neuroimaging metrics.Study TypeProspective/cross‐sectional.PopulationIn all, 49 subjects, including 13 with AD dementia, 12 with mild cognitive impairment (MCI), and 24 healthy controls (HC).Field Strength/Sequence3T/magnetization‐prepared rapid acquisition gradient echo (MP‐RAGE) and IVIM‐DWIAssessmentAll participants completed a cognitive screening battery prior to MRI. IVIM‐DWI maps (pure diffusion coefficient [D], pseudodiffusion coefficient [D*], and perfusion fraction [f]) were generated from a biexponential fit of diffusion MRI data. VBM was performed on the standard T1‐weighted MP‐RAGE structural images. Group‐wise templates were used to compare across groups.Statistical TestsAnalysis of covariance (ANCOVA) with gender and age as covariates (familywise error [FWE] corrected, post‐hoc comparisons using Bonferroni correction) for group comparisons. Partial‐η2 and Hedges' g were used for effect‐size analysis. Spearman's correlations (false discovery rate [FDR]‐corrected) for the relationship between cognitive scores and imaging.ResultsClusters of significant group‐wise differences were found mainly in the temporal lobe, hippocampus, and amygdala using all VBM and IVIM methods (P < 0.05 FWE). While VBM showed significant changes between MCI and AD groups and between HC and AD groups, no significant clusters were observed between HC and MCI using VBM. ADC and IVIM‐D demonstrated significant changes, at P < 0.05 FWE, between HC and MCI, notably in the amygdala and hippocampus. Several voxel‐based correlations were observed between neuroimaging metrics and cognitive tests within the cognitively impaired groups (P < 0.05 FDR).Data ConclusionThese findings suggest that IVIM‐DWI metrics may be earlier biomarkers for AD‐related changes than VBM. The use of these techniques may provide novel insight into subvoxel neurodegenerative processes.Level of Evidence2Technical Efficacy Stage2 J. MAGN. RESON. IMAGING 2020;52:1811–1826.
Flavone as a novel matrix for the matrix‐assisted laser desorption/ionisation analysis of lanthanide and transition metal salts
Authors: Warren Alexander D. , Gates Paul J. 
Published: July 2020

AbstractThe mass spectral analysis of metal salts, especially lanthanide and transition metal salts, can be challenging. Although getting information on the metal present is usually straightforward, obtaining information on the correct oxidation state and anion composition is challenging. Many ionisation techniques have some redox component to the ionisation process, which commonly results in changing the oxidation state of the metal and the associated loss of ligand and anion information. We present here a simple method for negative ion matrix‐assisted laser desorption/ionisation mass spectrometry using the non‐acidic flavonoid flavone as a novel matrix. This results in reliable information on the oxidation state of the metal as spectra are dominated by anion adduct ions with very little (typically no) redox processes occurring.
Integrating ion mobility and imaging mass spectrometry for comprehensive analysis of biological tissues: A brief review and perspective
Authors: Rivera Emilio S. , Djambazova Katerina V. , Neumann Elizabeth K. , Caprioli Richard M. , Spraggins Jeffrey M. 
Published: July 2020

AbstractImaging mass spectrometry (IMS) technologies are capable of mapping a wide array of biomolecules in diverse cellular and tissue environments. IMS has emerged as an essential tool for providing spatially targeted molecular information due to its high sensitivity, wide molecular coverage, and chemical specificity. One of the major challenges for mapping the complex cellular milieu is the presence of many isomers and isobars in these samples. This challenge is traditionally addressed using orthogonal liquid chromatography (LC)‐based analysis, though, common approaches such as chromatography and electrophoresis are not able to be performed at timescales that are compatible with most imaging applications. Ion mobility offers rapid, gas‐phase separations that are readily integrated with IMS workflows in order to provide additional data dimensionality that can improve signal‐to‐noise, dynamic range, and specificity. Here, we highlight recent examples of ion mobility coupled to IMS and highlight their importance to the field.
Establishment of a rapid ELISPOT assay for influenza virus titration and neutralizing antibody detection
Authors: Wang Guosong , Huang Pengfei , Hong Junping , Fu Rao , Wu Qian , Chen Ruiqi , Lin Lina , Han Qiangyuan , Chen Honglin , Chen Yixin , Xia Ningshao 
Published: July 2020

AbstractSeasonal influenza is an acute respiratory infection causing around 500 000 global deaths annually. There is an unmet medical need to develop more effective antiviral drugs and vaccines against influenza infection. A rapid, accurate, high‐throughput titration assay for influenza virus particles or neutralizing antibodies would be extremely useful in these research fields. However, commonly used methods such as tissue culture infective dose and plaque‐forming units (PFU) for virus particle quantification, and the plaque reduction neutralization test (PRNT) for antibody determination are time‐consuming, laborious, and have limited accuracy. In this study, we developed an efficient assay based on the enzyme‐linked immunospot (ELISPOT) technique for the influenza virus and neutralizing antibody titration. Two broad‐spectrum antibodies recognizing the nucleoproteins of influenza A and B viruses were used in the assay to broadly and highly sensitively detect influenza virus‐infected cells at 16 hours postinfection. An optimized cell culture medium with no tosyl phenylalanyl chloromethyl ketone trypsin and high dose oseltamivir acid was used to improve quantitation accuracy. This ELISPOT assay displayed a good correlation (R2 = 0.9851) with the PFU assay when used to titrate 30 influenza virus isolates. The assay was also applied to measure influenza‐neutralizing antibodies in 40 human sera samples, showing a good correlation (R2 = 0.9965) with the PRNT assay. This ELISPOT titration assay is a rapid, accurate, high‐throughput assay for quantification of influenza virus and neutralizing antibodies, and provides a powerful tool for research into and development of drugs and vaccines against influenza.
Long‐term Impact of Middle Ear Effusion in Pediatric Tympanostomy Tubes
Authors: Kaffenberger Thomas M. , Belsky Michael A. , Oberlies Nicholas R. , Kumar Aarti , Donohue Joseph P. , Yang Tiffany S. , Shaffer Amber D. , Chi David H. 
Published: July 2020

Objectives/HypothesisBilateral myringotomy and tympanostomy tube placement (BMT) is the most common pediatric surgery in the United States. Intraoperative middle ear effusion (MEE) is a risk factor for future BMTs in children with recurrent acute otitis media (RAOM). However, the impact of the type of MEE is unknown. Here, we assess otologic outcomes based on intraoperative MEE type and indication for surgery.Study DesignCase series chart review.MethodsAfter institutional review board approval, we performed a review of children undergoing BMTs between 2008 and 2009. Included patients had their first BMT, preoperative visit, and an operative report. Patients with cleft palate or Down syndrome were excluded. Indications for surgery included RAOM and chronic otitis media with effusion (COME). Other variables evaluated were future BMT, acquired cholesteatoma, and otorrhea. Logistic regression was used for statistical analysis.ResultsOut of 1,045 patients reviewed, 680 were included and underwent their first BMT. There were 619 patients who had RAOM. Serous effusions were present in 22.2%, mucoid in 31.3%, purulent in 12.9%, undocumented or bloody in 2.3% of patients, and 31.2% of patients had dry middle ears. Moreover, 22.7% of patients underwent future BMTs. In RAOM patients, serous effusions decreased odds of perforation (odds ratio [OR]: 0.195, 95% confidence interval [CI]: 0.0438‐0.867, P = .032), and purulent effusions increased the odds of in‐office otorrhea suctioning (OR: 2.13, 95% CI: 1.20‐3.77, P = .010) compared to dry. Mucoid effusions had no significant effect on outcomes in COME or RAOM patients.ConclusionsIntraoperative MEEs were noted in 68.7% of cases; purulent effusions increase the odds of in‐office suctioning in RAOM patients.Level of Evidence4 Laryngoscope, 131:E993–E997, 2021