bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Biofilm Inhibitory Potential of Compound Produced from Chlamydomonas reinhardtii Against Pathogenic Microorganisms
Authors: Et al. A. 
Published: March 2020

Microalgae present much usefulness for antimicrobial research because of its enormous biodiversity and rapid growth rate. From this study results it is reaveled that Chlamydomonas reinhardtii were isolated from a pond of water in the province of Diwaniyah. The culture supernatants were obtained when extracted with methanol solvent. Antimicrobial activity of extracts was tested for pathogens, and the best inhibition zone obtained was against Candida albicans (32mm), S.aureus (15mm), and to E.coli (9mm). While it showed no effect against both S.epidermidis and Klebsiella spp. Biofilm was formed by all tested isolates with differences in its strength formation. The C. reinhardtii Algal extract showed higher reduction of the existing Staphylococcus aureus biofilm and (10.9 %) was the remaining biofilm, while 55.21% and 54.98% were to Serratia spp. and Escherichia coli respectively, and it’s the lowest reduction. Klebsiella spp., Candida albicans, Acinetobacter spp., Staphylococcus epidermides, and Aspergillus sp. showed remaining biofilm as (16.7, 18.3, 19.6, 43.6, and 44.1) % respectively. The composition of the volatile compounds of the C. reinhardtii extract was determined by GC/MS. Different groups of compounds were identified such as hydrocarbons, phenols, alcohols and esters, and two bioactive compounds;1-Heptacosanol and Octadecyl chloride which is used in medical and pharmaceutical fields.These results provide an indication of the existence of hopeful antibiofilm compounds in the algal species under study. Further chemical studies are required to illuminate these compounds, structures and activity.
The Spatial Analysis for Bassia eriophora (Schrad.) Asch. Plant Distributed in all IRAQ by Using RS & GIS Techniques
Authors: Et al. Al Ramahi 
Published: March 2020

Most vegetation’s are Land cover (LC) for the globe, and there is an increased attention to plants since they represent an element of balance to natural ecology and maintain the natural balance of rapid changes due to systematic and random human uses, including the subject of the current study (Bassia eriophora ) Which represent an essential part of the United Nations system for land cover classification (LCCS), developed by the World Food Organization (FAO) and the world Organization for environmental program (UNEP), to observe basic environmental elements with modern techniques. Although this plant is distributed all over Iraq, we found that this plant exists primarily in the middle and southern parts of Iraq in wet areas and near rivers or catchment area or water basins. The main idea is how to use this techniques to monitor the distribution of the plants (Bassia eriophora), in order to utilize and take provisions of plant growth and diffusion, Moreover, know and control the breadth of these areas in Iraq and prepare for them, and understanding climates and the variable plantation habitats and mapping patterns, may lead to a successful environmental protection and dominance plan. In this study, monitoring the distribution of Bassia eriophora in Iraq by apply remote sensing (RS) and geographic information systems (GIS) techniques. The data was obtained from (BAG) National Herbarium of Iraq, Ministry of Agriculture. (BUNH) Iraq Natural History Research Centre and Museum, University of Baghdad the University Herbarium (BUH) in College of Science at University of Baghdad. This data contains only the address (place of germination), this information (addresses) was detected, identified and covered for all areas sampled by the techniques of satellite imagery and images taken from the air and some data records where the main features of these areas.
Psychiatric morbidity and poor follow-up underlie suboptimal functional and survival outcomes in Huntington's disease
Authors: Ratna Nikhil , Kamble Nitish L , V Sowmya D , Purushottam Meera , Pal Pramod K , Jain Sanjeev 
Published: March 2020

Abstract
BACKGROUND: Huntington’s disease (HD), an inherited, often late-onset, neurodegenerative disorder, is considered to be a rare, orphan disease. Research into its genetic correlates and services for those affected are inadequate in most low-middle income countries, including India. The apparent ‘incurability’ often deters symptomatic and rehabilitative care, resulting in poor quality of life and sub-optimal outcomes. There are no studies assessing disease burden and outcomes from India.
METHODS: We attempted to evaluate individuals diagnosed to have HD at our tertiary-care center between 2013 and 2016 for clinical symptoms, functionality, mortality, follow up status through a structured interview, clinical data from medical records and UHDRS-TFC scoring.
RESULTS: Of the 144 patients, 25% were untraceable, and another 17 (11.8%) had already died. Mean age at death and duration of illness at the time of death, were 53 years and 7 years respectively, perhaps due to suicides and other comorbidities at an early age. The patients who could be contacted (n=81) were assessed for morbidity and total functional capacity (TFC). Mean CAG repeat length and TFC score were 44.2 and 7.5 respectively. Most individuals (66%) were in TFC stage I and II and could perhaps benefit from several interventions. The TFC score correlated inversely with duration of illness (p<0.0001). The majority were being taken care of at home, irrespective of the physical and mental disability. There was a high prevalence of psychiatric morbidity (91%) including suicidal tendency (22%). Three of the 17 who died had committed suicide, and several other families reported suicidal history in other family members. Only about half the patients (57%) maintained a regular clinical follow-up.
CONCLUSIONS: This study demonstrates the poor follow-up rates, significant suicidality and other psychiatric symptoms, sub-optimal survival durations and functional outcomes highlighting the need for holistic care for the majority who appear to be amenable to interventions.
Evaluating the impact of the weather conditions on the influenza propagation
Authors: Singh David E. , marinescu maria-cristina , Carretero Jesus , Delgado-Sanz Concepcion , Gomez-Barroso Diana , Larrauri Amparo 
Published: March 2020

Abstract
Background: Predicting the details of how an epidemic evolves is highly valuable as health institutions need to better plan towards limiting the infection propagation effects and optimizing their prediction and response capabilities. Simulation is a cost- and time-effective way of predicting the evolution of the infection as the joint influence of many different factors: interaction patterns, personal characteristics, travel patterns, meteorological conditions, previous vaccination, etc. The work presented in this paper extends EpiGraph, our influenza epidemic simulator, by introducing a meteorological model as a modular component that interacts with the rest of EpiGraph's modules to refine our previous simulation results. Our goal is to estimate the effects of changes in temperature and relative humidity on the patterns of epidemic influenza based on data provided by the Spanish Influenza Sentinel Surveillance System (SISSS) and the Spanish Meteorological Agency (AEMET). Methods: Our meteorological model is based on the regression model developed by Barreca and Shimshack, and it is tuned with influenza surveillance data obtained from SISSS. After pre-processing this data to clean it and reconstruct missing samples, we obtain new values for the reproduction number of each urban region in Spain, every 10 minutes during 2011. We simulate the propagation of the influenza by setting the date of the epidemic onset and the initial influenza-illness rates for each urban region. Results: We show that the simulation results have the same propagation shape as the weekly influenza rates as recorded by SISSS. We perform experiments for a realistic scenario based on actual meteorological data from 2010-2011, and for synthetic values assumed under simplified predicted climate change conditions. Results show that a diminishing relative humidity of 10\% produces an increment of about 1.6\% in the final infection rate. The effect of temperature changes on the infection spread is also noticeable, with a decrease of 1.1\% per extra degree. Conclusions: Using a tool like ours could help predict the shape of developing epidemics and its peaks, and would permit to quickly run scenarios to determine the evolution of the epidemic under different conditions. We make EpiGraph source code and epidemic data publicly available.
Effect of arsenic stress on 5-methylcytosine, photosynthetic parameters and nutrient content in arsenic hyperaccumulator Pteris cretica (L.) var. Albo-lineata
Authors: Zemanová Veronika , Popov Marek , Pavlíková Daniela , Kotrba Pavel , Hnilička František , Česká Jana , Pavlík Milan 
Published: March 2020

Abstract
Background: Arsenic toxicity induces a range of metabolic responses in plants, including DNA methylation. The focus of this paper was on the relationship between As-induced stress and plant senescence in the hyperaccumulator Pteris cretica var. Albo-lineata (Pc-Al). We assume difference in physiological parameters and level of DNA methylation in young and old fronds as symptoms of As toxicity.Results: The As accumulation of Pc-Al fronds, grown in pots of haplic chernozem contaminated with 100 mg As kg-1 for 122 days, decreased with age. Content of As was higher in young than old fronds for variants with 100 mg As kg-1 (2800 and 2000 mg As kg-1 dry matter, respectively). The highest As content was determined in old fronds of Pc-Al grown in pots with 250 mg As kg-1. The increase with age was confirmed for determined nutrients – Cu, Mg, Mn, S and Zn. A significant elevation of all analysed nutrients was showed in old fronds. Arsenic accumulation affected DNA methylation status in fronds, but content of 5-methylcytosine (5mC) decreased only in old fronds of Pc-Al (from 25% to 12%). Determined photosynthetic processes showed a decrease of fluorescence, photosynthetic rate and chlorophylls of As treatments in young and old fronds. Water potential was decreased by As in both fronds. Thinning of the sclerenchymatous inner cortex and a reduction in average tracheid metaxylem in the vascular cylinder was showed in roots of As treatment. Irrespective to fronds age, physiological parameters positively correlated with a 5mC while negatively with direct As toxicity. Opposite results were found for contents of Cu, Mg, Mn, S and Zn.Conclusions: The results of this paper point to changes in the metabolism of the hyperaccumulator plant Pc-Al, upon low and high exposure to As contamination. The significant impact of As on DNA methylation was found in old fronds. Irrespective to fronds age, significant correlations were confirmed for 5mC and As toxicity. Our analysis of the very low water potential values and lignification of cell walls in roots showed that transports of assimilated metabolites and water between roots and fronds were reduced. As was showed by our results, epigenetic changes could affect studied parameters of the As hyperaccumulator plant Pc-Al, especially in old fronds.
High-throughput Sequencing Study of the Effect of Transabdominal Hysterectomy on Intestinal Flora in Patients with Uterine Fibroids
Authors: Wang Wantong , Li Yibing , Wu Qijun , Pan Xin , He Xinhui , Ma Xiaoxin 
Published: March 2020

Abstract
Background: To investigate the effect of transabdominal hysterectomy on the diversity of the intestinal flora in patients with uterine fibroids. Patients with uterine fibroids were selected from September 2018 to December 2018, in the Department of Obstetrics and Gynecology, Shengjing Hospital, China Medical University, and stool specimens were collected from patients before and after surgery. Results :High-throughput sequencing of the 16S rRNA gene was used to detect the changes in microbial community structure and diversity, and the effects of total hysterectomy on the intestinal flora were further analyzed. Estrogen levels decreased after trans-abdominal hysterectomy. High-throughput sequencing showed that after abdominal hysterectomy, the abundance and diversity of the intestinal flora decreased. The abundance changes were mainly due to Proteobacteria, where their abundance increased. Conclusions :Trans-abdominal hysterectomy changes the intestinal flora of the body by lowering the level of estrogen in the body, which reduces the diversity and abundance of the intestinal flora .
Oxidative stress and gut-derived lipopolysaccharides in children affected by Paediatric Autoimmune Neuropsychiatric Disorders Associated with Streptococcal infections.
Authors: Loffredo Lorenzo , Spalice Alberto , Salvatori Francesca , Giovanna De Castro , Guido Cristiana Alessia , Zicari Anna Maria , Ciacci Paolo , Battaglia Simona , Brindisi Giulia , Ettorre Evaristo , Nocella Cristina , Salvatori Guglielmo , Duse Marzia , Violi Francesco , Carnevale Roberto 
Published: March 2020

Abstract
Background: pediatric autoimmune neuropsychiatric disorders associated with streptococcal infections syndrome (PANDAS) identifies patients with acute onset of obsessive-compulsive and tic disorders. The objective of this study was to assess NOX2 levels, as well as serum 8-iso-prostaglandin F2α (iso-PGF2α) and lipopolysaccharide (LPS) derived from Gram-negative bacteria in the gut of patients with PANDAS.

Methods: a cross sectional study was performed to compare serum levels of soluble NOX2-dp (sNOX-2-dp), isoprostanes and LPS in 60 consecutive subjects, including 30 children affected by PANDAS and 30 controls (CT) matched for age and gender. Serum zonulin was used to assess gut permeability.
Results: compared with CT, PANDAS children had higher values of sNOX-2-dp, 8-iso-PGF2-alpha and LPS. Simple linear regression analysis showed that sNOX2-dp was signiﬁcantly correlated with serum LPS (Rs=0.359; p=0.005), zonulin (Rs=0.444; p<0.001) and iso-PGF2α (Rs=0.704; p<0.001). LPS signiﬁcantly correlated with serum zonulin (Rs=0.610; p<0.001), and iso-PGF2α (Rs=0.591; p=0.001). A multiple linear regression analysis was performed to define the independent predictors of sNOX-2-dp. Isoprostanes and zonulin emerged as the only independent predictive variables associated with sNOX2-dp (R2=68%).
Conclusion: this study provides evidences that children affected by PANDAS have high circulating levels of sNOX2-dp, isoprostanes and of LPS that could be potentially implicated in the process of neuroinflammation.
How are systematic reviews of prevalence conducted? A methodological study
Authors: Migliavaca Celina Borges , Stein Cinara , Colpani Verônica , Barker Timothy Hugh , Munn Zachary , Falavigna Maicon 
Published: March 2020

Abstract
Background: There is a notable lack of methodological and reporting guidance for systematic reviews of prevalence data. This information void has the potential to result in reviews that are inconsistent and inadequate to inform healthcare policy and decision making. The aim of this meta-epidemiological study is to describe the methodology of recently published prevalence systematic reviews.
Methods: We searched MEDLINE (via PubMed) from February 2017 to February 2018 for systematic reviews of prevalence studies. We included systematic reviews assessing the prevalence of any clinical condition using patients as the unit of measurement and we summarized data related to reporting of and methodology of the reviews.
Results: A total of 235 systematic reviews of prevalence were analyzed. The median number of authors was 5 (interquartile range [IQR] 4-7), the median number of databases searched was 4 (3-6) and the median of studies included in each review was 24 (IQR 15-41.5). Search strategies were presented for 68% of reviews. Forty five percent of reviews received external funding, and 24% did not provide funding information. Twenty three percent of included reviews had published or registered the systematic review protocol. Reporting guidelines were used in 72% of reviews. The quality of included studies was assessed in 80% of reviews. Twelve reviews assessed the overall quality of evidence (50% of them using GRADE). Meta-analysis was conducted in 65% of reviews; 1% used Bayesian methods. Random effect meta-analysis was used in 94% of reviews; among them, 75% did not report the variance estimator used. Among the reviews with meta-analysis, 70% did not report how data was transformed; 59% percent conducted subgroup analysis, 38% conducted meta-regression and 2% estimated prediction interval; I 2 was estimated in 95% of analysis. Publication bias was examined in 48%. The most common software used was STATA (55%).
Conclusions: Our results indicate that there are significant inconsistencies regarding how these reviews are conducted. Many of these differences arose in the assessment of methodological quality and the formal synthesis of comparable data. This variability indicates the need for clearer reporting standards and consensus on methodological guidance for systematic reviews of prevalence data.
A genetic variant in the promoter of lncRNA MALAT1 is related to susceptibility of ischemic stroke
Authors: Wang Yan , Gu Xi-Xi , Huang Hua-Tuo , Liu Chun-Hong , Wei Ye-Sheng 
Published: March 2020

Abstract
Background: Metastasis-associated lung adenocarcinoma transcript-1 ( MALAT1 ) was aberrantly expressed in diverse diseases. Particularly in ischemic stroke (IS), the abnormal expression of MALAT1 played important roles including promotion of angiogenesis, inhibition of apoptosis and inflammation and regulation of autophagy. However, the effects of genetic variation (single nucleotide polymorphisms, SNPs) of MALAT1 on IS have rarely been explored. This study aimed to investigate whether SNPs in promoter of MALAT1 were associated with the susceptibility to IS.
Methods: A total of 316 IS patients and 320 age-, gender-, and ethnicity-matched controls were enrolled in this study. Four polymorphisms in the promoter of MALAT1 (i.e., rs600231, rs1194338, rs4102217, and rs591291) were genotyped by using a custom-by-design 48-Plex SNPscan kit.
Results: The rs1194338 C>A variant in the promoter of MALAT1 was associated with the risk of IS (AC vs. CC: adjusted OR = 0.623, 95% CI, 0.417-0.932, P = 0.021; AA vs. CC: adjusted OR = 0.474, 95% CI, 0.226-0.991, P = 0.047; Dominant model: adjusted OR = 0.596, 95% CI, 0.406-0.874, P = 0.008; A vs. C adjusted OR = 0.658, 95% CI, 0.487-0.890, P = 0.007). The haplotype analysis showed that rs600231-rs1194338-rs4102217-rs591291 (A-C-G-C) had a 1.3-fold increased risk of IS (95% CI, 1.029-1.644, P = 0.027). Logistic regression analysis identified some independent impact factors for IS including rs1194338 AC/AA, TC, TG, HDL-C, LDL-C, Apo-A1, Apo-B and NEFA ( P < 0.05).
Conclusions: These results suggest that the rs1194338 AC/AA genotypes may be a protective factor for IS.
Keywords: Ischemic stroke, Polymorphism, Metastasis associated lung adenocarcinoma transcript 1
The impact of peri-natal stress on psychosis risk: results from the Bo-FEP incidence study
Authors: De Matteis Tiziano , D'Andrea Giuseppe , Lal Jatin , Berardi Domenico , Tarricone Ilaria 
Published: March 2020

Abstract
Objective: According to the gene-environment interaction model the pathogenesis of psychosis relies on an adverse neuro-socio-developmental pathway. Perinatal stress represents an important risk factor for the development of psychosis because of the increasingly evident interference with socio-neuro-development in the earlier phases of life. We aim to investigate the correlation of perinatal risk factors with the onset of psychosis with a case-control – incidence study.
Results: Patients (and their mothers) were eligible if they presented with first-episode psychosis at the Bologna West Community Mental Health Centre (Bo-West CMHC) between 2002 and 2012. The Bo-West CMHC serves a catchment area of about 200,000 people. The controls were recruited in the same catchment area and study period. 42 patients, 26 controls and their mothers were included. We collected the history of peri-natal stress and calculated crude and adjusted Odds Ratios for onset of first-episode psychosis. Adjusted logistic regression showed that psychosis onset was significantly associated with stressful situations during pregnancy, lower level of maternal physical health before or during pregnancy, use of anti-inflammatory drugs during pregnancy, and low level of maternal education. The results of our study suggest that stress during perinatal period increases the risk of developing psychosis.
Ethno-veterinary practices of Poaceae taxa in Punjab, Pakistan
Authors: Majeed Muhammad , Bhatti Khizar Hayat , Amjad Muhammad Shoaib , Abbasi Arshad Mehmood , Rashid Audil , Nawaz Fahim , Mehmood Ansar , Mahmood Majid , Ahmad Khawaja Shafique 
Published: March 2020

Abstract
Background: Plant species of Poaceae family are not only used as fodder and forage but also contribute substantially in the treatment of various health disorders, particularly in livestock. Consequently, present study was aimed to document the therapeutic uses Poaceae taxa by the inhabitants of the Punjab Province to treat various veterinary health disorders. Methods: Semi structured interviews, group discussion and field walks were conducted to collected data. Furthermore, quantitative indices includingcultural significance index (CSI), relative frequency of citations (RFC), fidelity level (FL), and relative popularity level (RPL) andJaccard Index (JI) were adopted for data analysis. Results: Traditional uses of 149 plants belonging to 60 genera, 16 tribes of 5 sub families of Poaceae were recorded. Whole plant and leaves were the most consistent grazing parts with 40.94 and 29.53% contribution and decoction (35 reports) was the most preferred mode of administration. Majority of the plant species were employed to treat infectious diseases (25.93 %). and digestive disorders (14.10 %). Triticum aestivum depicted highest CSI, RFC and RPL levels at 8.00, 0.96, 1.00, respectively, followed by Oryza sativa and Poa annua . Likewise, T. aestivum and Saccharum spontaneum had 100 % FL and ROP. Jaccard index ranged from 12.25 to 0.37. Twelve plant species namely Chrysopogon zizanioides (anti-inflammatory), Pennisetum lansatum (improve bull fertility), Cymbopogon citratus (glandular secretion), Sorghum saccharatum and Themeda triandra (malaria), Aristida funiculate (anticancer) , Koeleria argentia (skin allergies), Tetrapogon villosus (antibacterial), Cynodon radiates (eyes infection), Sporobolus nervosa (Jaundice), Enneapogon persicus (antifungal), and Panicum repens (dysfunctional cattle organs) were reported for the first time with novelethnoveterinary uses. Conclusion: Inhabitants of the study area had strong association with surrounding plant biodiversity and possess significant knowledge on therapeutic uses of grasses and other members of Poaceae to treat various health disorders in animals. Plant species with maximum cultural and medicinal values could be a potential source of novel drugs to cure health disorders in animals and human as well.