bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impact of female mating status and female familiarity with remating interval on the reproductive success of Propylea dissecta (Mulsant) (Coleoptera: Coccinellidae)
Authors: Singh Priya , Mishra Geetanjali , Omkar  
Published: March 2020

Abstract
Remating rate is a fundamental parameter that acts on disease transmission, sexual dimorphism, and the rate of evolution of species. Recent studies have indicated that sperm production can be costly. It is thus likely that males may tailor their sperm expenditure according to female mating status and the remating interval between successive matings. In this study, we investigated the effects of male remating interval, female mating status and familiarity of females in the ladybird beetle Propylea dissecta (Mulsant). Ten-day-old adults were allowed to mate and, post disengagement, these adults were exposed to second mating opportunities, either immediately after the first mating, or 6, 18 or 24 h later. To assess the effect of female mating status, the males were subjected to mating with virgin and mated females. Similarly, for assessing the effects of female familiarity, males were subjected to mating with either familiar or unfamiliar females. With increasing remating interval individuals mated for longer, resulting in higher fecundity. Percent egg viability increased with increased remating interval and was highest at 24 h. Mated and unfamiliar females were found to be more fecund than virgin females. Mated females produced a higher number of viable eggs than virgins, suggesting that multiple mating and promiscuity are essential for maximizing the reproductive success of both sexes.
The millipede family Conotylidae in northwestern North America, with a complete bibliography of the family (Diplopoda, Chordeumatida, Heterochordeumatidea, Conotyloidea)
Authors: SHEAR WILLIAM A. , RICHART CASEY H. , WONG VICTORIA L. 
Published: March 2020

The center of diversity for the chordeumatidan millipede family Conotylidae is northwestern North America, where five of six subfamilies and ten of fourteen previously described genera occur; in this paper, seven additional new genera and 33 new species from the region are described. New species in the genus Taiyutyla Chamberlin, 1952 are: Taiyutyla tillamook, Taiyutyla acuphora, and Taiyutyla amicitia; in the genus Bollmanella Chamberlin, 1941: Bollmanella bombus, Bollmanella washingtonensis and Bollmanella leonardi; in the genus Brunsonia Loomis & Schmitt, 1971: Brunsonia pulchra, Brunsonia digitata, Brunsonia wenatchee, Brunsonia chelanoparva, Brunsonia chelanomagna, Brunsonia selwayana and Brunsonia benewah. Vancouvereuma n. gen. is described with the type species Taiyutyla shawi Shear 2004. Calityla n. gen. includes the new species Calityla siskiyou, Calityla ubicki, Calityla trinitaria, Calityla essigi, and Calityla humboldtensis. Ovaskella, n. gen. includes the new species Ovaskella ovaskae and Ovaskella sinuosa. Karagama, n. gen. includes the new species Karagama ladybird. Complicatella, n. gen. includes the new species Complicatella pectenifera and Complicatella neili. Bifurcatella n. gen. includes the new species Bifurcatella olympiana, Bifurcatella hoh, Bifurcatella angulata, Bifurcatella pacifica, Bifurcatella germania, Bifurcatella uniclada, Bifurcatella inflata and Bifurcatella hobo. Loomisiella, n. gen. includes the new species Loomisiella evergreen and Loomisiella pylei. New distribution records are provided for numerous previously described species of conotylids. A complete bibliography of the family Conotylidae of the world is also included.
Heterogeneous impact of cytomegalovirus reactivation on nonrelapse mortality in hematopoietic stem cell transplantation
Authors: Kaito Satoshi , Nakajima Yujiro , Hara Konan , Toya Takashi , Nishida Tetsuya , Uchida Naoyuki , Mukae Junichi , Fukuda Takahiro , Ozawa Yukiyasu , Tanaka Masatsugu , Ikegame Kazuhiro , Katayama Yuta , Kuriyama Takuro , Kanda Junya , Atsuta Yoshiko , Ogata Masao , Taguchi Ayumi , Ohashi Kazuteru 
Published: March 2020

Abstract
Cytomegalovirus (CMV) infection is a major complication in allogeneic stem cell transplantation. The utility of CMV prophylaxis with letermovir has been reported; however, the specific applications remain unclear. In this study, we retrospectively analyzed large-scale registry data (N = 10 480) to clarify the risk factors for nonrelapse mortality (NRM) in connection with CMV reactivation. First, we identified risk factors for CMV reactivation using multivariate analysis and developed a scoring model. Although the model effectively stratified reactivation risk into 3 groups (43.7% vs 60.9% vs 71.5%; P &lt; .001), the 3-year NRM was significantly higher in patients with CMV reactivation, even in the low (20.9% vs 13.0%, P &lt; .001), intermediate (21.4% vs 15.6%; P &lt; .001), and high (29.3% vs 18.0%; P &lt; .001) reactivation risk groups. Next, survival analysis considering competing risks, time-dependent covariates, and interaction terms for exploring the heterogeneous impact of CMV reactivation on NRM in the training cohort revealed that chronic myeloid leukemia (CML) (hazard ratio [HR], 1.76; 95% confidence interval [CI], 1.05-2.96; P = .033), good performance status (PS) (HR, 1.42; 95% CI, 1.04-1.94; P = .028), HLA-matched donor (HR, 1.34; 95% CI, 1.06-1.70; P = .013), and standard-risk disease (HR, 1.28; 95% CI, 1.04-1.58; P = .022) were associated with increased NRM. In the test cohort, CMV reactivation was significantly associated with increased 3-year NRM among patients with 2 to 4 factors (22.1% vs 13.1%; P &lt; .001) but was comparable among patients with 0 or 1 factor (23.2% vs 20.4%; P = .62). We propose that CMV prophylaxis should be determined based on reactivation risk, as well as these other factors.
The EGFL7-ITGB3-KLF2 axis enhances survival of multiple myeloma in preclinical models
Authors: Salama Yousef , Heida Andries Hendrik , Yokoyama Kazuaki , Takahashi Satoshi , Hattori Koichi , Heissig Beate 
Published: March 2020

Abstract
Angiogenic factors play a key role in multiple myeloma (MM) growth, relapse, and drug resistance. Here we show that malignant plasma cells (cell lines and patient-derived MM cells) express angiocrine factor EGF like-7 (EGFL7) mRNA and protein. MM cells both produced EGFL7 and expressed the functional EGFL7 receptor integrin β 3 (ITGB3), resulting in ITGB3 phosphorylation and focal adhesion kinase activation. Overexpression of ITGB3 or EGFL7 enhanced MM cell adhesion and proliferation. Intriguingly, ITGB3 overexpression upregulated the transcription factor Krüppel-like factor 2 (KLF2), which further enhanced EGFL7 transcription in MM cells, thereby establishing an EGFL7-ITGB3-KLF2-EGFL7 amplification loop that supports MM cell survival and proliferation. EGFL7 expression was found in certain plasma cells of patients with refractory MM and of patients at primary diagnosis. NOD.CB17-Prkdc&lt;scid&gt;/J mice transplanted with MM cells showed elevated human plasma EGFL7 levels. EGFL7 knockdown in patient-derived MM cells and treatment with neutralizing antibodies against EGFL7 inhibited MM cell growth in vitro and in vivo. We demonstrate that the standard-of-care MM drug bortezomib upregulates EGFL7, ITGB3, and KLF2 expression in MM cells. Inhibition of EGFL7 signaling in synergy with BTZ may provide a novel strategy for inhibiting MM cell proliferation.
Genomic profiles and clinical outcomes of de novo blastoid/pleomorphic MCL are distinct from those of transformed MCL
Authors: Jain Preetesh , Zhang Shaojun , Kanagal-Shamanna Rashmi , Ok Chi Young , Nomie Krystle , Gonzalez Graciela Nogueras , Gonzalez-Pagan Omarya , Hill Holly A. , Lee Hun Ju , Fayad Luis , Westin Jason , Nastoupil Loretta , Hagemeister Frederick , Chen Wendy , Oriabure Onyeka , Badillo Maria , Jiang Changying , Yixin Yao , Li Shaoying , Tang Guilin , Yin C. Cameron , Patel Keyur P. , Medeiros Leonard Jeffrey , Nair Ranjit , Ahmed Sairah , Iyer Swaminathan P. , Thirumurthi Selvi , Champlin Richard , Xu Guofan , Tinsu Pan , Santos David , Wang Ruiping , Han Guangchun , Zhang Jianhua , Song Xingzhi , Neelapu Sattva , Romaguera Jorge , Futreal Andy , Flowers Christopher , Fowler Nathan , Wang Linghua , Wang Michael L. 
Published: March 2020

AbstractBlastoid and pleomorphic mantle cell lymphomas (MCLs) are variants of aggressive histology MCL (AH-MCL). AH-MCL can arise de novo (AH-DN) or transform from prior classic variant MCL (AH-t). This study is the first integrated analysis of clinical and genomic characteristics of AH-MCL. Patient characteristics were collected from diagnosis (AH-DN) and at transformation (AH-t). Survival after initial diagnosis (AH-DN) and after transformation (AH-t) was calculated. Regression tree analysis was performed to evaluate prognostic variables and in univariate and multivariate analyses for survival. Whole-exome sequencing was performed in evaluable biopsy specimens. We identified 183 patients with AH-MCL (108 were AH-DN, and 75 were AH-t; 152 were blastoid, and 31 were pleomorphic). Median survival was 33 months (48 and 14 months for AH-DN and AH-t, respectively; P = .001). Factors associated with inferior survival were age (≥72 years), AH-t category, Ki-67 ≥50% and poor performance status. AH-t had a significantly higher degree of aneuploidy compared with AH-DN. Transformed MCL patients exhibited KMT2B mutations. AH-MCL patients with Ki-67 ≥50% had exclusive mutations in CCND1, NOTCH1, TP53, SPEN, SMARCA4, RANBP2, KMT2C, NOTCH2, NOTCH3, and NSD2 compared with low Ki-67 (&lt;50%). AH-t patients have poor outcomes and distinct genomic profile. This is the first study to report that AH-MCL patients with high Ki-67 (≥50%) exhibit a distinct mutation profile and very poor survival.
Correction to: Computational assembly of a human Cytomegalovirus vaccine upon experimental epitope legacy
Authors: Quinzo Monica J. , Lafuente Esther M. , Zuluaga Pilar , Flower Darren R. , Reche Pedro A. 
Published: March 2020

After publication of the original article [1], we were notified that legends of Fig. 1 and Fig. 2 have been swapped.
Diagnosis of bilateral diffuse uveal melanocytic proliferation unveils primary gastric adenocarcinoma: a case report
Authors: Luo Mingyue , Chen Zhe , Luo Yaping , Zhao Lin , Dai Rongping , Zhong Yong 
Published: March 2020

Abstract
Background
Bilateral diffuse uveal melanocytic proliferation (BDUMP) is an extremely rare paraneoplastic syndrome, with most cases reported as secondary to female urogenital and male lung malignancies. We reported this case of BDUMP patient whose primary malignancy was gastric adenocarcinoma verified with gastroscopy and subsequent pathological test.

Case presentation
A patient complaining blurred vision was suspected of bilateral diffuse uveal melanocytic proliferation (BDUMP), due to bilateral round oval patches at the posterior pole and cardinal signs in retinal angiography. Malignancy screening was suggested, and pathological report from gastroscopy confirmed the primary lesion as gastric adenocarcinoma. The patient chose palliative care due to late stage and unresectable nature of the malignancy.

Conclusions
Identifying BDUMP warrants further investigation of a primary malignancy. Our case provided evidence for the link between gastric adenocarcinoma and BDUMP.
A simultaneous bilateral quadriceps and patellar tendons rupture in patients with chronic kidney disease undergoing long-term hemodialysis: a case report
Authors: Tao Zhengbo , Liu Wenbo , Ma Weifeng , Luo Peng , Zhi Shengpeng , Zhou Renyi 
Published: March 2020

Abstract
Background
The incidence of rupture of the quadriceps or patellar tendon s is low, especially that of bilateral quadriceps tendon rupture, and it is generally considered a complication secondary to chronic systemic disorders. We report two rare cases of simultaneous bilateral tendon rupture affecting the extensor function of the knee in patients with chronic kidney disease who have been treated with long-term haemodialysis.

Case presentation
Two young males with a history of chronic kidney disease who were being treated with long-term haemodialysis presented to our hospital with clinical signs of disruption of the extensor mechanism of the knee. One patient was diagnosed with bilateral quadriceps tendon rupture, and the other patient had bilateral patellar tendon rupture. They underwent surgical repair of the tendons, and their knees were actively mobilized during physiotherapy.

Conclusion
Bilateral quadriceps or patellar tendons rupture is a rare occurrence in patients with chronic kidney disease who are being treated with long-term haemodialysis. Timely surgical treatment and scientific physiotherapy can lead to good recovery of knee joint function.
Exploring optimal examination to detect occult anastomotic leakage after rectal resection in patients with diverting stoma
Authors: Kitaguchi Daichi , Enomoto Tsuyoshi , Ohara Yusuke , Owada Yohei , Hisakura Katsuji , Akashi Yoshimasa , Takahashi Kazuhiro , Ogawa Koichi , Shimomura Osamu , Oda Tatsuya 
Published: March 2020

Abstract
Background
When considering “early stoma closure”, both standardized inclusion/exclusion criteria and standardized methods to assess anastomosis are necessary to reduce the risk of occult anastomotic leakage (AL). However, in the immediate postoperative period, neither have the incidence and risk factors of occult AL in patients with diverting stoma (DS) been clarified nor have methods to assess anastomosis been standardized. The aim of this study was to elucidate the incidence and risk factors of occult AL in patients who had undergone rectal resection with DS and to evaluate the significance of computed tomography (CT) following water-soluble contrast enema (CE) to detect occult anastomotic leakage.

Methods
This was a single institutional prospective observational study of patients who had undergone rectal resection with the selective use of DS between May and October 2019. Fifteen patients had undergone CE and CT to assess for AL on postoperative day (POD) 7, and CT was performed just after CE. Univariate analysis was performed to assess the relationship between preoperative variables and the incidence of occult AL on POD 7.

Results
The incidence of occult AL on postoperative day 7 was 6 of 15 (40%). Hand-sewn anastomosis, compared with stapled anastomosis, was a significant risk factor. Five more cases with occult AL that could not be detected with CE could be detected on CT following CE; CE alone had a 33% false-negative radiological result rate.

Conclusions
Hand-sewn anastomosis appeared to be a risk factor for occult AL, and CE alone had a high false-negative radiological result rate. When considering the introduction of early stoma closure, stapled anastomosis and CT following CE could be an appropriate inclusion criterion and preoperative examination, respectively.
Detection of adeABC efllux pump encoding genes and antimicrobial effect of Mentha longifolia and Menthol on MICs of imipenem and ciprofloxacin in clinical isolates of Acinetobacter baumannii
Authors: Mahmoudi Hassan , Shokoohizadeh Leili , Zare Fahim Nayreh , Mohamadi Bardebari Ali , Moradkhani Shirin , Alikhani Mohammad Yousef 
Published: March 2020

AbstractBackgroundAcinetobacter baumanniiis an opportunistic pathogen that causes nosocomial infections especially in patients in intensive care units (ICUs). Accordingly, the aim of our study was to detectionof adeABCefllux pump encoding genes and antimicrobial effect of the essential oil ofMentha longifoliaandMentholon the minimum inhibitory concentration (MIC) of imipenem and ciprofloxacin in clinical isolates ofA. baumannii.MethodsA total of 75 clinical isolates ofA. baumanniiwere collected. The presence of efflux pump genes was detected by polymerase chain reaction (PCR). The minimum inhibitory concentration (MIC) of the essential oil ofMentha longifoliaand Menthol and their combined effect with antibiotics were measured by microbroth dilution method and fractional inhibitory concentration (FIC) index.ResultsThe frequency ofadeA,adeB, andadeCgenes in clinical isolates ofA. baumanniiwere 86.7, 90.7, and 92%, respectively. When the essential oil ofMentha longifoliawas combined with ciprofloxacin and imipenem, MICs decreased 4- and 8-fold, respectively. In the combination of menthol with imipenem, the resistance to imipenem was reduced from 0- to 16-fold in 90% (63/70) of the isolates.ConclusionThe presence of efflux pump genes in more than 90% ofA. baumanniiisolates indicates its potential role in inducing imipenem- and ciprofloxacin-resistance in this bacterium. Menthol has an antimicrobial effect as an active ingredient inMentha longifolia. In the future, the combination of medicinal plants with antibiotics can be used as a complement in treating diseases caused by drug-resistant bacteria such asA. baumanniiinfections.
Preventing major adverse cardiovascular events by SGLT-2 inhibition in patients with type 2 diabetes: the role of kidney
Authors: Giugliano Dario , De Nicola Luca , Maiorino Maria Ida , Bellastella Giuseppe , Garofalo Carlo , Chiodini Paolo , Ceriello Antonio , Esposito Katherine 
Published: March 2020

AbstractCardiovascular outcome trials (CVOTs) have demonstrated a significant reduction of major adverse cardiovascular events (MACE) in patients with type 2 diabetes (T2D) treated by SGLT-2 inhibitors. This holds true in the presence of background therapy with statins in most patients. Noteworthy, this SGLT-2 inhibitors effect is unique because, at variance with other components of cardiorenal protection, MACE prevention does not appear to be a class effect. Here, we present meta-analysis of the four key CVOTs indicating a major role of renal function in determining the extent of MACE prevention, with the benefit increasing in more severe kidney disease, that is, a high-risk condition where effectiveness of the traditional approach with statins is reduced.
Anti-HSV-1 activity of Aspergillipeptide D, a cyclic pentapeptide isolated from fungus Aspergillus sp. SCSIO 41501
Authors: Wang Zhaoyang , Jia Jiaoyan , Wang Lu , Li Feng , Wang Yiliang , Jiang Yuzhou , Song Xiaowei , Qin Shurong , Zheng Kai , Ye Ju , Ren Zhe , Wang Yifei , Qi Shuhua 
Published: March 2020

Abstract
Background
Herpes simplex virus 1, an enveloped DNA virus belonging to the Herpesviridae family, spreads to neurons and causes pathological changes in the central nervous system. The purpose of this study was to investigate the potency and mechanism of antiviral activity of Aspergillipeptide D, a cyclic pentapeptide isolated from a culture broth of marine gorgonian-derived fungus Aspergillus sp. SCSIO 41501, At present, there are many studies on the anti-tumor, anti-clotting, anti-oxidant and immunoinflammatory effects of Aspergillipeptide D, but little research has been done on the anti-HSV-1 activity of Aspergillipeptide D.

Methods
The anti-HSV-1 activity of Aspergillipeptide D was evaluated by plaque reduction assay. The mechanism of action against HSV-1 was determined from the effective stage. Then we assayed the viral DNA replication, viral RNA synthesis and protein expression, respectively. We also identified the proteins that interact with gB by mass spectrometry, and assayed the effect of Aspergillipeptide D on the interaction between the virus gB protein and cell proteins.

Results
Plaque reduction experiments showed that Aspergillipeptide D did not affect HSV-1 early infection events, including viral inactivation, attachment and penetration. Interestingly, Aspergillipeptide D dramatically reduced both the gene and protein levels of viral late protein gB, and suppressed its location in the endoplasmic reticulum and Golgi apparatus. In contrast, overexpression of gB restored viral production. Finally, proteomic analysis revealed that the numbers of cellular proteins that interacted with gB protein was largely decreased by Aspergillipeptide D. These results suggested that Aspergillipeptide D inhibited gB function to affect HSV-1 intercellular spread.

Conclusions
Our results indicated that Aspergillipeptide D might be a potential candidate for HSV-1 therapy, especially for ACV-resistant strains.
Influence on [18F]FDG uptake by cancer cells after anti-PD-1 therapy in an enforced-immune activated mouse tumor
Authors: Tomita Mayu , Suzuki Motofumi , Kono Yusuke , Nakajima Kohei , Matsuda Takuma , Kuge Yuji , Ogawa Mikako 
Published: March 2020

Abstract
Background
Anti-programmed cell death 1 (PD-1) antibody is an immune checkpoint inhibitor, and anti-PD-1 therapy improves the anti-tumor functions of T cells and affects tumor microenvironment. We previously reported that anti-PD-1 treatment affected tumor glycolysis by using 2-deoxy-2-[18F]fluoro-D-glucose ([18F]FDG) positron emission tomography (PET). That study showed that anti-PD-1 therapy in a mouse B16F10 melanoma model increased glucose metabolism in cancer cells at the point where anti-PD-1 therapy did not cause a significant inhibition of tumor growth. However, the B16F10 melanoma model is poorly immunogenic, so it is not clear how anti-PD-1 treatment affects glucose metabolism in highly immunogenic cancer models. In this study, we used a cyclic dinucleotide GMP-AMP (cGAMP)-injected B16F10 melanoma model to investigate the effect of anti-PD-1 therapy on [18F]FDG uptake in a highly immune activated tumor in mice.

Results
To compare the cGAMP-injected B16F10 model with the B16F10 model, experiments were performed as described in our previous manuscript. [18F]FDG-PET was measured before treatment and 7 days after the start of treatment. In this study, [18F]FDG uptake in tumors in the cGAMP/anti-PD-1 combination group was lower than that in the anti-PD-1 treatment group tumors on day 7, as shown by PET and ex vivo validation. Flow-cytometry was performed to assess immune cell populations and glucose metabolism. Anti-PD-1 and/or cGAMP treatment increased the infiltration level of immune cells into tumors. The cGAMP/anti-PD-1 combination group had significantly lower levels of GLUT1high cells/hexokinase IIhigh cells in CD45− cancer cells compared with tumors in the anti-PD-1 treated group. These results suggested that if immune responses in tumors are higher than a certain level, glucose uptake in cancer cells is reduced depending on that level. Such a change of glucose uptake might be caused by the difference in infiltration or activation level of immune cells between the anti-PD-1 treated group and the cGAMP/anti-PD-1 combination group.

Conclusions
[18F]FDG uptake in cancer cells after anti-PD-1 treatment might be affected by the tumor immune microenvironment including immune cell infiltration, composition, and activation status.
Nonlocal-derivative NLS equations and group-invariant soliton solutions
Authors: Yao Yuqin , Huang Yehui 
Published: March 2020

AbstractA coupled Chen–Lee–Liu (CLL) system is proposed and its linear Lax pair is given. Many kinds of nonlocal-derivative NLS (DNLS) equations arise from the group symmetry reductions of the coupled CLL system. $\hat{P}\hat{T}\hat{C}$PˆTˆCˆ-symmetry invariant one-soliton solution and periodic two-soliton solution of a two-place DNLS (TDNLS) system are obtained. A group symmetry invariant two-soliton solution of a four-place DNLS (FDNLS) system is worked out. New characteristics of the two-soliton interactions for the TDNLS system and FDNLS system are analyzed.