bluuebunny/crossref_metadata_2025_split huggingface dataset:
Biomarker testing in oncology – Requirements for organizing external quality assessment programs to improve the performance of laboratory testing: revision of an expert opinion paper on behalf of IQNPath ABSL
Authors: Dufraing K. , Fenizia F. , Torlakovic E. , Wolstenholme N. , Deans Z. C. , Rouleau E. , Vyberg M. , Parry S. , Schuuring E. , Dequeker Elisabeth M. C. ,   on behalf of IQNPath ABSL, Normanno N. , Cheetham M. H. , Patton S. , Keppens C. , van Casteren K. , van Krieken J. H. , Fairley J. A. , Grassow-Narlik M. , Jöhrens K. , Pagliuso J. 
Published: October 2020

AbstractIn personalized medicine, predictive biomarker testing is the basis for an appropriate choice of therapy for patients with cancer. An important tool for laboratories to ensure accurate results is participation in external quality assurance (EQA) programs. Several providers offer predictive EQA programs for different cancer types, test methods, and sample types. In 2013, a guideline was published on the requirements for organizing high-quality EQA programs in molecular pathology. Now, after six years, steps were taken to further harmonize these EQA programs as an initiative by IQNPath ABSL, an umbrella organization founded by various EQA providers. This revision is based on current knowledge, adds recommendations for programs developed for predictive biomarkers by in situ methodologies (immunohistochemistry and in situ hybridization), and emphasized transparency and an evidence-based approach. In addition, this updated version also has the aim to give an overview of current practices from various EQA providers.
Patient ambassador support in newly diagnosed patients with acute leukemia during treatment: a feasibility study
Authors: Nørskov Kristina Holmegaard , Overgaard Dorthe , Boesen Jannie , Struer Anne , El-Azem Sarah Elke Weber Due , Tolver Anders , Lomborg Kirsten , Kjeldsen Lars , Jarden Mary 
Published: October 2020

Abstract
Purpose
This study investigated the feasibility of patient ambassador support in newly diagnosed patients with acute leukemia during treatment.

Methods
A multicenter single-arm feasibility study that included patients newly diagnosed with acute leukemia (n = 36) and patient ambassadors previously treated for acute leukemia (n = 25). Prior to the intervention, all patient ambassadors attended a 6-h group training program. In the intervention, patient ambassadors provided 12 weeks of support for patients within 2 weeks of being diagnosed. Outcome measures included feasibility (primary outcome), safety, anxiety, and depression measured by the Hospital Anxiety and Depression Scale, quality of life by the Functional Assessment of Cancer Therapy–Leukemia and the European Organization for Research and Treatment of Cancer Quality of Life Questionnaire, and symptom burden by MD Anderson Symptom Inventory, the Patient Activation Measure, and the General Self-Efficacy Scale.

Results
Patient ambassador support was feasible and safe in this population. Patients and patient ambassadors reported high satisfaction with the individually adjusted support, and patients improved in psychosocial outcomes over time. Patient ambassadors maintained their psychosocial baseline level, with no adverse events, and used the available support to exchange experiences with other patient ambassadors and to manage challenges.

Conclusion
The patient ambassador support program is feasible and has the potential to be a new model of care incorporated in the hematology clinical care setting, creating an active partnership between patients and former patients. This may strengthen the existing supportive care services for patients with acute leukemia.

Trial registration
NCT03493906
Enhancing seed conservation in rural communities of Guatemala by implementing the dry chain concept
Authors: Guzzon Filippo , Bello Pedro , Bradford Kent J. , Mérida Guzman María de los Angeles , Costich Denise E. 
Published: October 2020

AbstractSeed conservation in rural communities of low- and middle-income countries located in tropical areas is particularly problematic, due to high relative humidity that promotes insect and fungal infestations and leads to rapid losses in seed viability. Seed conservation in those areas is affected by unreliable power supplies that do not allow the use of dehumidifying and refrigeration systems recommended for the long-term storage of seeds. We tested the dry chain, i.e., initial seed drying with a reusable desiccant in the form of zeolite beads followed by seed conservation in hermetic containers, in rural communities of Guatemala (Huehuetenango Department). In this region, a network of community seed reserves (CSRs) has been established to provide a safety backup for seed and to conserve local agrobiodiversity. Using a local maize variety in three communities, we compared the dry chain with the seed conservation methodology employed in the CSRs (i.e., undried seeds in hermetic flasks) as well as with seed conservation in open storage, both in the local CSR and in a farmer’s granary. Seed conserved using the dry chain treatment maintained very high seed viability (> 80%) throughout the whole experiment (6 months) and reduced fungal and insect infestations (< 3%). In the other treatments, the viability declined significantly to an average of 52% non-viable and 19% infested seeds after 6 months of storage. The dry chain was demonstrated to be an excellent solution for enhancing seed conservation in biodiversity hotspots of tropical areas as well as for improving seed security for farmers.
Myocardial adaptation as assessed by speckle tracking echocardiography after isolated mitral valve surgery for primary mitral regurgitation
Authors: Gerçek Muhammed , Faber Lothar , Rudolph Volker , Fox Henrik , Puehler Thomas , Omran Hazem , Wolf Lisa Katharina , Paluszkiewicz Lech , Zeiher Andreas M. , Hakim-Meibodi Kavous , Gummert Jan , Dimitriadis Zisis 
Published: October 2020

AbstractThe risk of left ventricular (LV) and right ventricular (RV) maladaptation after surgery for isolated primary mitral regurgitation (PMR) is poorly defined. We aimed to evaluate LV and RV contractile function using speckle-tracking analysis alongside with quantification of exercise tolerance in patients with PMR after mitral valve surgery. All consecutive patients with symptomatic PMR undergoing mitral valve surgery between July 2015 and May 2017 were prospectively enrolled. Sequential echocardiographic studies along with clinical assessment were performed before and three months after surgery. Mean age in 138 patients was 65.8 ± 12.7 years, 48.2% (66) of whom were female. Mean LV ejection fraction decreased from 57 ± 12% to 50 ± 11% (p < 0.001), LV global longitudinal strain deteriorated from −19.2 ± 4.1% to −15.7 ± 3.8% (p < 0.001), and mechanical strain dispersion increased from 88 ± 12 to 117 ± 115 ms (p = 0.004). There was a reduction in tricuspid annulus plane systolic excursion from 22 ± 5 mm to 18 ± 4 mm (p < 0.001), as well as a slight deterioration of RV free wall mean longitudinal strain from −16.9 ± 5.6% to −15.7 ± 4.1% (p = 0.05). The rate of moderate to severe tricuspid regurgitation significantly decreased (p < 0.005). Regarding exercise tolerance, the New York Heart Association class improved (p < 0.001) and the walking distance increased (p < 0.001). During mid-term follow up after surgery for PMR, a deterioration of LV and RV contractile function measures could be observed. However, the clinical status, LV dimensions, and concomitant tricuspid regurgitation improved which in particular imply more effective RV contractile pattern.
Novel Anti-inflammatory Effects of Canagliflozin Involving Hexokinase II in Lipopolysaccharide-Stimulated Human Coronary Artery Endothelial Cells
Authors: Uthman Laween , Kuschma Marius , Römer Gregor , Boomsma Marleen , Kessler Jens , Hermanides Jeroen , Hollmann Markus W. , Preckel Benedikt , Zuurbier Coert J. , Weber Nina C. 
Published: October 2020

Abstract
Purpose
Vascular inflammation and disturbed metabolism are observed in heart failure and type 2 diabetes mellitus. Glycolytic enzyme hexokinase II (HKII) is upregulated by inflammation. We hypothesized that SGLT2 inhibitors Canagliflozin (Cana), Empagliflozin (Empa) or Dapagliflozin (Dapa) reduces inflammation via HKII in endothelial cells, and that HKII-dependent inflammation is determined by ERK1/2, NF-κB. and/or AMPK activity in lipopolysaccharide (LPS)-stimulated human coronary artery endothelial cells (HCAECs).

Methods
HCAECs were pre-incubated with 3 μM or 10 μM Cana, 1 μM, 3 μM or 10 μM Empa or 0.5 μM, 3 μM or 10 μM Dapa (16 h) and subjected to 3 h LPS (1 μg/mL). HKII was silenced via siRNA transfection. Interleukin-6 (IL-6) release was measured by ELISA. Protein levels of HK I and II, ERK1/2, AMPK and NF-κB were detected using infra-red western blot.

Results
LPS increased IL-6 release and ERK1/2 phosphorylation; Cana prevented these pro-inflammatory responses (IL-6: pg/ml, control 46 ± 2, LPS 280 ± 154 p < 0.01 vs. control, LPS + Cana 96 ± 40, p < 0.05 vs. LPS). Cana reduced HKII expression (HKII/GAPDH, control 0.91 ± 0.16, Cana 0.71 ± 0.13 p < 0.05 vs. control, LPS 1.02 ± 0.25, LPS + Cana 0.82 ± 0.24 p < 0.05 vs. LPS). Empa and Dapa were without effect on IL-6 release and HKII expression in the model used. Knockdown of HKII by 37% resulted caused partial loss of Cana-mediated IL-6 reduction (pg/ml, control 35 ± 5, LPS 188 ± 115 p < 0.05 vs. control, LPS + Cana 124 ± 75) and ERK1/2 activation by LPS. In LPS-stimulated HCAECs, Cana, but not Empa or Dapa, activated AMPK. AMPK activator A769662 reduced IL-6 release.

Conclusion
Cana conveys anti-inflammatory actions in LPS-treated HCAECs through 1) reductions in HKII and ERK1/2 phosphorylation and 2) AMPK activation. These data suggest a novel anti-inflammatory mechanism of Cana through HKII.
A survey of community detection methods in multilayer networks
Authors: Huang Xinyu , Chen Dongming , Ren Tao , Wang Dongqi 
Published: October 2020

AbstractCommunity detection is one of the most popular researches in a variety of complex systems, ranging from biology to sociology. In recent years, there’s an increasing focus on the rapid development of more complicated networks, namely multilayer networks. Communities in a single-layer network are groups of nodes that are more strongly connected among themselves than the others, while in multilayer networks, a group of well-connected nodes are shared in multiple layers. Most traditional algorithms can rarely perform well on a multilayer network without modifications. Thus, in this paper, we offer overall comparisons of existing works and analyze several representative algorithms, providing a comprehensive understanding of community detection methods in multilayer networks. The comparison results indicate that the promoting of algorithm efficiency and the extending for general multilayer networks are also expected in the forthcoming studies.
A chronic strain of the cystic fibrosis pathogen Pandoraea pulmonicola expresses a heterogenous hypo-acylated lipid A
Authors: Pither Molly D. , McClean Siobhán , Silipo Alba , Molinaro Antonio , Di Lorenzo Flaviana 
Published: October 2020

AbstractPandoraea sp. is an emerging Gram-negative pathogen in cystic fibrosis causing severe and persistent inflammation and damage of the lungs. The molecular mechanisms underlying the high pathogenicity of Pandoraea species are still largely unknown. As Gram-negatives, Pandoraea sp. express lipopolysaccharides (LPS) whose recognition by the host immune system triggers an inflammatory response aimed at the bacterial eradication from the infected tissues. The degree of the inflammatory response strongly relies on the fine structure of the LPS and, in particular, of its glycolipid moiety, i.e. the lipid A. Here we report the structure of the lipid A isolated from the LPS of a chronic strain of P. pulmonicola (RL 8228), one of the most virulent identified so far among the Pandoraea species. Our data demonstrated that the examined chronic strain produces a smooth-type LPS with a complex mixture of hypoacylated lipid A species displaying, among other uncommon characteristics, the 2-hydroxylation of some of the acyl chains and the substitution by an additional glucosamine on one or both the phosphate groups.
Sandstone Ground Stone Technology: a Multi-level Use Wear and Residue Approach to Investigate the Function of Pounding and Grinding Tools
Authors: Cristiani E. , Zupancich A. 
Published: October 2020

AbstractGround stone tool (GST) technology includes artefacts utilized in pounding or grinding activities and characterized by long life cycles and multiple uses. The introduction of such technology dates back to early prehistory, and for this reason, it is used as prime evidence for tackling a wide range of archaeological questions such as the origins of technology, patterns of daily subsistence and lifeways. In this paper, we contribute to the field of study of GSTs by discussing the application of a novel multi-level analytical approach combining use wear and residue observations at low and high magnification with residue spatial distribution investigated using GIS. We aim to assess the potential of a combined use wear and residue approach to (1) discriminate specific gestures and worked materials on sandstone GSTs and (2) understand the potential of residue distributions for reconstructing ancient GST functions and, more specifically, the role of GSTs recovered at Mesolithic sites of the Danube Gorges region in the Central Balkans. We identified diagnostic surface modifications associated with the use of GSTs in different activities involving both animal and plant materials. Morphological characteristics of residues have been described under reflected and transmitted light and characterized using biochemical staining. Finally, we mapped residues across the utilized surfaces of experimental tools to link patterns of residue spatial distribution to specific materials and gestures. We expect our methodological approach will be taken up by other researchers in order to produce comparable datasets on GSTs and improve our understanding of their ancient functional biographies.
The Dawn of the Mesolithic on the Plains of Poland
Authors: Płonka Tomasz , Bobak Dariusz , Szuta Michał 
Published: October 2020

AbstractIn this article we take a fresh look at the population dynamics of the Polish Plain in the transition from the Pleistocene to the Holocene, using Bayesian analysis and modelling of radiocarbon dates, and contrast the results with data from the North German Plain. We argue against simple adaptationalist models and instead see the cultural landscape as a complex patchwork of old forms and the emerging new traits of the early Mesolithic. We argue that the Mesolithic directly follows the Final Palaeolithic on the Polish Plain, without the chronological hiatus of 150–300 years that is often assumed for that region; while, by contrast, the two cultural patterns—Final Palaeolithic and microlith-based Mesolithic—overlapped significantly in time on the adjacent North German Plain.
Mechanochemical and microwave treatment of precipitated zirconium dioxide and study of its physical–chemical, thermal and photocatalytic properties
Authors: Kucio Karolina , Sydorchuk Volodymyr , Khalameida Svitlana , Charmas Barbara 
Published: October 2020

AbstractThe effects of the microwave treatment (MWT) and mechanochemical treatment (MChT) on the structure and physicochemical properties of precipitated zirconium oxide were investigated. The obtained materials were characterized using the N2 adsorption/desorption, thermogravimetry (TG, DTG, DTA), XRD and UV–Vis/DRS methods. Photocatalytic properties of the samples were also studied as regards the rhodamine B (RhB) degradation in the aqueous solution. The results show that the microwave and MChT, differing in mill rotation speed, temperature or treatment media, causes significant changes in the porous structure of the obtained samples. In most samples, the specific surface area increase was observed. The DTA and XRD results showed the formation of crystalline structure during MWT. As follows from the investigations, each way of modification results in the shift of the absorption edge toward higher wavelength values and causes photocatalytic degradation of RhB under UV irradiation and makes the obtained materials effective photocatalysts in the visible region.
La notion de tiers-espace et la traduction juridique : quelle articulation ?
Authors: Flöter-Durr Margarete , Nowak-Korcz Paulina 
Published: October 2020

AbstractIn recent years, we have noticed in many research areas a growing interest in the translation paradigm. In this article we discuss the theory of “cultural translation”, developed by Homi Bhabha in the context of postcolonial studies, and his concept of “Third Space” (2007). This theory aims to describe the different processes at play in the creation of identity within a space in which several cultures coexist. According to Bhabha, “the Third Space, though unrepresentable in itself, constitutes the discursive conditions of enunciation that ensure that the meaning and symbols of culture have no primordial unity or fixity; even the same signs can be appropriated, translated, rehistorised and read anew”. In this article, we aim to examine to what extent this theory can be applied to translation, especially to legal translation. The concepts of “Third Space” and hybridity developed by Bhabha undoubtedly have a certain appeal for the translator. However, from the methodological point of view other approaches seem to allow for a better understanding of the translation aspects raised by Bhabha in his theory. We therefore provide an interesting analysis of the “Third Space” based on the concept ofintervalby Cassin (Éloge de la traduction. Compliquer l’universel, Fayard, Paris, 2016), which is defined as a complex zone of interactions and interferences. This analysis is completed by examining the concept of frontiers by Moréteau (Revue internationale de droit comparé 4(61):695–713, 2009. DOI: 10.3406/ridc.2009.19911) which is implicitly present in the concept ofinterval. In order to analyse the concept of hybridity, the methodological framework of transdifference developed by Srubar (Kultur und Semantik, VS Verlag für Sozialwissenschaften, Wiesbaden, 2009) can also be used.
Social dimensions of a forest-based bioeconomy: A summary and synthesis
Authors: Ranacher Lea , Wallin Ida , Valsta Lauri , Kleinschmit Daniela 
Published: October 2020

AbstractHow perceptions of the forest-based bioeconomy differ across country contexts and social groups is important as it opens possibilities for the development of more inclusive, locally and socially relevant bioeconomy policies and strategies. Therefore, this special section explores the social dimensions of the forest-based bioeconomy by focusing on discourses and perceptions of different actor groups in Europe. We introduce six articles that range from review and discursive approaches to consumer studies. The section adds to the existing literature by focusing not only on political decision makers, stakeholders, and experts but also on the public, media and students. Patterns in the presented discourses and perceptions can be identified but more is needed to validate these and respond to the question of representativeness.
Recommendations for the treatment of epilepsy in adult and pediatric patients in Belgium: 2020 update
Authors: Boon Paul , Ferrao Santos Susana , Jansen Anna C. , Lagae Lieven , Legros Benjamin , Weckhuysen Sarah 
Published: October 2020

AbstractTo guide health care professionals in Belgium in selecting the appropriate antiepileptic drugs (AEDs) for their epilepsy patients, a group of Belgian epilepsy experts developed recommendations for AED treatment in adults and children (initial recommendations in 2008, updated in 2012). As new drugs have become available, others have been withdrawn, new indications have been approved and recommendations for pregnant women have changed, a new update was pertinent. A group of Belgian epilepsy experts (partly overlapping with the group in charge of the 2008/2012 recommendations) evaluated the most recent international guidelines and relevant literature for their applicability to the Belgian situation (registration status, reimbursement, clinical practice) and updated the recommendations for initial monotherapy in adults and children and add-on treatment in adults. Recommendations for add-on treatment in children were also included (not covered in the 2008/2012 publications). Like the 2008/2012 publications, the current update also covers other important aspects related to the management of epilepsy, including the importance of early referral in drug-resistant epilepsy, pharmacokinetic properties and tolerability of AEDs, comorbidities, specific considerations in elderly and pregnant patients, generic substitution and the rapidly evolving field of precision medicine.
Capturing the wisdom of the crowd: health professions’ educators meet at a&nbsp;virtual world café
Authors: McKimm Judy , Ramani Subha , Kusurkar Rashmi A. , Fornari Alice , Nadarajah Vishna Devi , Thampy Harish , Filipe Helena P. , Kachur E. K. , Hays Richard 
Published: October 2020

Background Conversations about educational challenges and potential solutions among a globally and culturally diverse group of health professions’ educators can facilitate identity formation, mentoring relationships and professional network building. The COVID-19 pandemic has made it even more important to co-create and disseminate knowledge, specifically regarding online and flexible learning formats.
Approach Based on the principles of social learning, we combined speed mentoring and world café formats to offer a virtual Zoom™ workshop, with large and small group discussions, to reach health professions’ educators across the globe. The goal was to establish a psychologically safe space for dialogue regarding adaptation to online teaching-learning formats.
Evaluation We aimed to establish psychological safety to stimulate thought-provoking discussions within the various small groups and obtain valuable contributions from participants. From these conversations, we were able to formulate ‘hot tips’ on how to adapt to (sometimes new) online teaching-learning formats while nurturing teacher and student wellbeing.
Reflection Through this virtual workshop we realized that despite contextual differences, many challenges are common worldwide. We experienced technological difficulties during the session, which needed rapid adaptation by the organising team. We encouraged, but did not pressure, participants to use video and audio during breakout discussions as we wanted them to feel safe and comfortable. The large audience size and different time zones were challenging; therefore, leadership had to be resilient and focussed. Although this virtual format was triggered by the pandemic, the format can be continued in the future to discuss other relevant global education topics.