bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Relevance of Ancient Science to the Survival of Secularism
Authors: Carrier Richard 
Published: April 2020

Abstract
To oppose Secularism modern Christians depend on myths about the historical development of civilization. Such as the myth of a Christian America, imagining such things as that the United States Constitution was based on Biblical Christian principles. Parallel to this myth is another about science: that the Scientific Revolution, and therefore modern science, was based on Biblical Christian principles and could not have occurred (and therefore cannot continue) without them. Necessary to this are several false claims, most particularly that ancient pagans never did and never could have made any significant scientific progress, and that Christian theology was essential to doing so. These myths are here dispelled with recourse to a survey of the actual facts of the matter.
“God’s Not My Type”
Authors: Galen Luke W. 
Published: April 2020

Abstract
The recent report by the Pew Forum on Religion and Public Life is intended to “offer a new and complementary lens with which to glean new insights into religion and public life”. The technique of cluster analysis was used on measures of religious and spiritual beliefs, yielding seven groups, two consisting primarily of nonreligious or secular individuals. There are “breadth-versus-depth” tradeoffs involved in this approach. A belief-based typology is an improvement upon a grouping that uses religious denomination, which undercounts secular individuals. But the theoretical implications of this typology for understanding secular individuals necessitate scrutiny, including how the use of meaning in life as a marker of well-being may be misleading in the case of the nonreligious.
Serum Renin Levels in Sudden Sensorineural Hearing Loss
Authors: Yıldız Muhammet , Baki̇ Ahmet , Özer Ömer Faruk 
Published: April 2020

Objective: The aim of this study was to investigate the serum renin levels of patients with idiopathic sudden sensorineural hearing loss (ISSNHL). Material and Methods: Twenty-four patients with ISSNHL and 24 asymptomatic healthy volunteers were included in the study. Subjects underwent pure-tone audiometry and serum renin levels were measured. Results: There were 14 women (mean age:42.35 ± 9.53) and 10 men (mean age:43.8 ± 6.87) in the patient group. There were 14 women (mean age:42.4 ± 4.7) and 10 men (mean age:41.4 ± 4.59) in the control group. ISSNHL was detected on the right side in 13 patients and on the left side in 11 patients. Serum renin levels of the patients and controls were 788.01 ± 327.8 and 282.37 ± 107.73 pg/mL, respectively. The serum renin levels were found to be significantly higher in the patient group compared to the control group ( P ≤ .001). There was a statistically significant strong positive correlation between serum renin level and the severity of hearing loss ( r = 0.77; P = .001). Conclusion: Serum renin levels of patients with ISSNHL were higher than controls. There was a statistically significant strong positive correlation between serum renin level and the severity of hearing loss.
Acute mitragynine administration suppresses cortical oscillatory power and systems theta coherence in rats
Authors: Thériault Rachel-Karson , Manduca Joshua D , Blight Colin R , Khokhar Jibran Y , Akhtar Tariq A , Perreault Melissa L 
Published: April 2020

Background: Mitragynine is the major alkaloid of Mitragyna speciosa (kratom) with potential as a therapeutic in pain management and in depression. There has been debate over the potential side effects of the drug including addiction risk and cognitive decline. Aims: To evaluate the effects of mitragynine on neurophysiological systems function in the prefrontal cortex (PFC), cingulate cortex (Cg), orbitofrontal cortex, nucleus accumbens (NAc), hippocampus (HIP), thalamus (THAL), basolateral amygdala (BLA) and ventral tegmental area of rats. Methods: Local field potential recordings were taken from animals at baseline and for 45 min following mitragynine administration (10 mg/kg, intraperitoneally). Drug-induced changes in spectral power and coherence between regions at specific frequencies were evaluated. Mitragynine-induced changes in c-fos expression were also analyzed. Results: Mitragynine increased delta power and reduced theta power in all three cortical regions that were accompanied by increased c-fos expression. A transient suppression of gamma power in PFC and Cg was also evident. There were no effects of mitragynine on spectral power in any of the other regions. Mitragynine induced a widespread reduction in theta coherence (7–9 Hz) that involved disruptions in cortical and NAc connectivity with the BLA, HIP and THAL. Conclusions: These findings show that mitragynine induces frequency-specific changes in cortical neural oscillatory activity that could potentially impact cognitive functioning. However, the absence of drug effects within regions of the mesolimbic pathway may suggest either a lack of addiction potential, or an underlying mechanism of addiction that is distinct from other opioid analgesic agents.
Health-related quality of life among elderly individuals living alone in an urban area of Shaanxi Province, China: a cross-sectional study
Authors: Liu Ning , Andrew Nadine E. , Cadilhac Dominique A. , Yu Xuewen , Li Zhe , Wang Jue , Liang Yu 
Published: April 2020

Objectives To describe health-related quality of life (HRQoL), sex differences in HRQoL, and factors affecting the HRQoL of elderly people living alone in urban areas of Shaanxi Province, China. Methods A cohort was obtained using multistage stratified cluster random sampling. We collected cross-sectional data using surveys. HRQoL was measured using the 36-item Short Form Health Survey. Multivariable multilevel linear regression analysis was used to examine factors associated with mental and physical health component summary scores. Results Overall, 442 elderly Chinese individuals who lived alone completed the survey (mean age 73 years, women: 59%). Women were more likely to report better mental health than men. Factors significantly associated with reduced mental health scores were older age, having never been married or being divorced, and having a history of cancer, urinary tract disease, fractures or gastrointestinal disorders. Factors associated with physical health were having never been married or being divorced and having hypertension. Conclusions To our knowledge, this is the first study to describe HRQoL in a representative sample of elderly people living alone in urban areas of Shaanxi, China. Poor physical and mental HRQoL in this elderly Chinese population was mainly associated with chronic diseases and demographic factors.
Effects of benzydamine hydrochloride on postoperative sore throat after extubation in children: a randomized controlled trial
Authors: Yhim Hyung-Been , Yoon Soo-Hyuk , Jang Young-Eun , Lee Ji-Hyun , Kim Eun-Hee , Kim Jin-Tae , Kim Hee-Soo 
Published: April 2020

AbstractBackgroundPostoperative sore throat (POST) is a common, undesirable result of endotracheal intubation during general anaesthesia. This study aimed to evaluate the effectiveness of benzydamine hydrochloride (BH) spray in reducing the incidence of POST in paediatric patients.MethodsThis randomized, double-blind, prospective study included 142 children 6–12 years of age, who were randomly assigned to receive either BH spray or control. After induction of anaesthesia, direct laryngoscope was placed and BH spray was applied to the upper trachea and vocal cord in the BH group and intubation was performed using a cuffed tube lubricated with normal saline. Intubation in the control group was performed using a cuffed tube lubricated with normal saline without any intervention. The balloon was inflated to a pressure of 20 cmH2O. Patients were extubated after fully awakened and transferred to the post-anaesthetic care unit (PACU), where they were examined for the presence of POST and any adverse events 30 min after arrival to the PACU. Postoperative pain was evaluated using a smartphone application.ResultsSeventy-one patients were allocated to each group. The incidence of POST in the BH group did not differ from that in the control group (control: BH = 35 (49.3%): 42 (59.2%);P = 0.238); postoperative pain was also similar between the groups. Other complications, such as breath holding, secretions, coughing, laryngospasm and desaturation events, did not differ between the groups.ConclusionsApplication of prophylactic BH spray to the vocal cords and upper trachea was not proven to reduce POST in paediatric patients.Trial registryNCT03074968 (ClinicalTrials.gov, Feb 26, 2017).
Effect of corneal cross-linking on endothelial cell density and morphology in the peripheral cornea
Authors: Goukon Hiroyasu , Kamiya Kazutaka , Takahashi Masahide , Shoji Nobuyuki 
Published: April 2020

AbstractBackgroundTo compare the endothelial cell density and morphology in the peripheral cornea before and aftercorneal cross-linking (CXL).MethodsThis study evaluated thirty-one eyes of 31 patients who were treated with standard CXL for progressive keratoconus. Preoperatively and 6 months postoperatively, we compared the corneal endothelial cell density (ECD), the coefficient of variation in cell size (CV), and the percentage of hexagonal cells (HEX), in the peripheral regions of the cornea, using a non-contact specular microscope (EM-3000, Tomey).ResultsAll keratoconic eyes in this series were measurable in the peripheral regions. No significant differences were found in the peripheral ECD preoperatively and 6 months postoperatively at each point (Wilcoxon signed-rank test, superior,p = 0.16, nasal superior,p = 0.12, temporal superior,p = 0.17, inferior,p = 0.37, nasal inferior,p = 0.28, temporal inferior,p = 0.17). The mean percentage of the ECD loss was 1.3, 1.3, 1.0, 1.4, 0.7, and 1.4%, respectively. No significant differences in the peripheral CV or HEX were found preoperatively and 6 months postoperatively at each point.ConclusionsStandard CXL does not cause significant changes in endothelial cell density, polymegethism, or polymorphism, in the peripheral regions of the cornea. It is suggested that CXL is a minimally invasive surgical approach for progressive keratoconus, even in terms of peripheral endothelial cells.Trial registrationUniversity Hospital Medical Information Network Clinical Trial Registry (000031162).
Natural history of gastroesophageal reflux in infancy: new data from a prospective cohort
Authors: Curien-Chotard Marlène , Jantchou Prévost 
Published: April 2020

Abstract
Background
Gastroesophageal reflux (GER) is common in infants. Gastroesophageal reflux disease (GERD) is defined as GER leading to troublesome symptoms that affect daily functioning and/or complications. This study is aimed at determining the prevalence and progression of GER and GERD in a cohort of healthy term infants from birth to 12 months old.

Methods
We conducted a prospective cohort study including all full-term living neonates born at Besançon Teaching Hospital, France. Parents completed a clinical report form and the Infant Gastroesophageal Reflux Questionnaire-Revised (I-GERQ-R) at 1, 3, 6, 10, and 12 months of age. GER was defined as score ≥ 1 to the first question with I-GERQ-R score < 16, and GERD as score ≥ 1 to the first question with I-GERQ-R score ≥ 16. Regurgitation was based on the answer to the first question of the I-GERQ-R as anything coming out of the mouth daily.

Results
157/347 births were included (83 boys). The prevalence of regurgitation at least once a day was 45.7% overall. In total: 72, 69, 56, 18, and 13% of infants regurgitated at least once a day at 1, 3, 6, 10, and 12 months of age, respectively. Physiological GER affected 53, 59, 51, 16, and 12% of infants; GERD, 19, 9, 5, 2, and 2%, respectively. Two risk factors were identified: family history of GER and exposure to passive smoking. Treatment included dietary modification (14%) and pharmacotherapy (5%).

Conclusion
Physiological GER peaked at 3 months, GERD at 1 month. Most cases resolved on their own. GER and GERD are very common in the infant’s population and parents should be reassured/educated regarding symptoms, warning signs, and generally favorable prognosis. I-GERQ-R is useful to the clinical screening and follow up for GER and GERD.
First statement on preparation for the COVID-19 pandemic in large German Speaking University-based radiation oncology departments
Authors: Combs Stephanie E. , Belka Claus , Niyazi Maximilian , Corradini Stefanie , Pigorsch Steffi , Wilkens Jan , Grosu Anca L. , Guckenberger Matthias , Ganswindt Ute , Bernhardt Denise 
Published: April 2020

AbstractThe COVID-19 pandemic is challenging modern radiation oncology. At University Hospitals, we have a mandate to offer high-end treatments to all cancer patients. However, in times of crisis we must learn to prioritize resources, especially personnel. Compromising oncological outcome will blur all statistics, therefore all measures must be taken with great caution. Communication with our neighboring countries, within societies and between departments can help meet the challenge. Here, we report on our learning system and preparation measures to effectively tackle the COVID-19 challenge in University-Based Radiation Oncology Departments.
Altered microRNA profile during fracture healing in rats with diabetes
Authors: Takahara Shunsuke , Lee Sang Yang , Iwakura Takashi , Oe Keisuke , Fukui Tomoaki , Okumachi Etsuko , Arakura Michio , Sakai Yoshitada , Matsumoto Tomoyuki , Matsushita Takehiko , Kuroda Ryosuke , Niikura Takahiro 
Published: April 2020

AbstractBackgroundMicroRNAs (miRNAs) are a class of small non-coding RNA molecules that regulate gene expression. There is increasing evidence that some miRNAs are involved in the pathology of diabetes mellitus (DM) and its complications. We hypothesized that the functions of certain miRNAs and the changes in their patterns of expression may contribute to the pathogenesis of impaired fractures due to DM.MethodsIn this study, 108 male Sprague–Dawley rats were divided into DM and control groups. DM rats were created by a single intravenous injection of streptozotocin. Closed transverse femoral shaft fractures were created in both groups. On post-fracture days 5, 7, 11, 14, 21, and 28, miRNA was extracted from the newly generated tissue at the fracture site. Microarray analysis was conducted with miRNA samples from each group on post-fracture days 5 and 11. The microarray findings were validated by real-time polymerase chain reaction (PCR) analysis at each time point.ResultsMicroarray analysis revealed that, on days 5 and 11, 368 and 207 miRNAs, respectively, were upregulated in the DM group, compared with the control group. The top four miRNAs on day 5 were miR-339-3p, miR451-5p, miR-532-5p, and miR-551b-3p. The top four miRNAs on day 11 were miR-221-3p, miR376a-3p, miR-379-3p, and miR-379-5p. Among these miRNAs, miR-221-3p, miR-339-3p, miR-376a-3p, miR-379-5p, and miR-451-5p were validated by real-time PCR analysis. Furthermore, PCR analysis revealed that these five miRNAs were differentially expressed with dynamic expression patterns during fracture healing in the DM group, compared with the control group.ConclusionsOur findings will aid in understanding the pathology of impaired fracture healing in DM and may support the development of molecular therapies using miRNAs for the treatment of impaired fracture healing in patients with DM.
The effect of acupuncture on tumor growth and gut microbiota in mice inoculated with osteosarcoma cells
Authors: Xu Xiaoru , Feng Xiangru , He Min , Zhang Zepeng , Wang Jiajia , Zhu Haiyu , Li Tie , Wang Fuchun , Sun Mengmeng , Wang Zhihong 
Published: April 2020

Abstract
Background
Cancer is a complex systemic disease. As a key component of traditional Chinese medicine, acupuncture is a clinically proven medical treatment for many diseases, and it also has preventative effects as it balances the body, allowing it to self-regulate. For cancer patients, acupuncture is widely used as complementary therapy to boost the immune system and reduce the side effects of radiotherapy and chemotherapy. However, few studies have determined how acupuncture against cancer, especially in regulating the intestinal flora of the tumor-burdened mice.

Methods
We treated osteosarcoma tumor-burdened mice by using needling on different acupoints and acupoints combination, thereafter determined the effects of acupuncture on tumor growth by using imaging technology in vitro. In addition, intestinal bacteria were analyzed for further understanding the holistic and systemic treatment effects of acupuncture in osteosarcoma tumor-burdened mice.

Results
Acupuncture treatment can delay tumor growth and changes of intestinal bacteria in osteosarcoma tumor-burdened mice. In detail, the loss of body weight and the development of tumor volume of mice have been postposed by needling specific acupoints. In addition, acupuncture treatment has delayed the changes of the relative abundance of Bacteroidetes, Firmicutes and Candidatus Saccharibacteria at the phylum level. Moreover, the relative abundance of many bacteria (e.g., Catabacter, Acetatifactor and Aestuariispira) has been regulated by using acupuncture treatment, and the trend of structural changes of these bacteria at the genus level has also been postposed compared to that of the tumor-burdened mice model group.

Conclusion
Our results suggest that acupuncture may provide a systemic treatment for cancer. Our findings encourage new and extensive research into the effects of acupuncture on changes of the intestinal microbiome associated with the development of cancer.
Sahaj Samadhi Meditation versus a Health Enhancement Program for depression in chronic pain: protocol for a randomized controlled trial and implementation evaluation
Authors: Sud Abhimanyu , Nelson Michelle L. A. , Cheng Darren K. , Armas Alana , Foat Kirk , Greiver Michelle , Hosseiny Fardous , Katz Joel , Moineddin Rahim , Mulsant Benoit H. , Newman Ronnie I. , Rivlin Leon , Vasudev Akshya , Upshur Ross 
Published: April 2020

Abstract
Background
Despite the high prevalence of comorbid chronic pain and depression, this comorbidity remains understudied. Meditation has demonstrated efficacy for both chronic pain and depression independently, yet there have been few studies examining its effectiveness when both conditions are present concurrently. Furthermore, while meditation is generally accepted as a safe and effective health intervention, little is known about how to implement meditation programs within or alongside the health care system.

Methods
We will conduct a hybrid type 1 effectiveness–implementation evaluation. To measure effectiveness, we will conduct a randomized controlled trial comparing Sahaj Samadhi Meditation and the Health Enhancement Program in 160 people living with chronic pain, clinically significant depressive symptoms, and on long-term opioid therapy. Changes in depressive symptoms will be our primary outcome; pain severity, pain-related function, opioid use, and quality of life will be the secondary outcomes. The primary end point will be at 12 weeks with a secondary end point at 24 weeks to measure the sustainability of acute effects. Patients will be recruited from a community-based chronic pain clinic in a large urban center in Mississauga, Canada. The meditation program will be delivered in the clinical environment where patients normally receive their chronic pain care by certified meditation teachers who are not regulated health care providers. We will use a mixed-methods design using the multi-level framework to understand the implementation of this particular co-location model.

Discussion
Results of this hybrid evaluation will add important knowledge about the effectiveness of meditation for managing depressive symptoms in people with chronic pain. The implementation evaluation will inform both effectiveness outcomes and future program development, scalability, and sustainability.

Trial registration
ClinicalTrials.gov: NCT04039568. Registered on 31 July 2019.
Multilevel optimisation of anaerobic ethyl acetate production in engineered Escherichia coli
Authors: Bohnenkamp Anna C. , Kruis Aleksander J. , Mars Astrid E. , Wijffels Rene H. , van der Oost John , Kengen Servé W. M. , Weusthuis Ruud A. 
Published: April 2020

Abstract
Background
Ethyl acetate is a widely used industrial solvent that is currently produced by chemical conversions from fossil resources. Several yeast species are able to convert sugars to ethyl acetate under aerobic conditions. However, performing ethyl acetate synthesis anaerobically may result in enhanced production efficiency, making the process economically more viable.

Results
We engineered an E. coli strain that is able to convert glucose to ethyl acetate as the main fermentation product under anaerobic conditions. The key enzyme of the pathway is an alcohol acetyltransferase (AAT) that catalyses the formation of ethyl acetate from acetyl-CoA and ethanol. To select a suitable AAT, the ethyl acetate-forming capacities of Atf1 from Saccharomyces cerevisiae, Eat1 from Kluyveromyces marxianus and Eat1 from Wickerhamomyces anomalus were compared. Heterologous expression of the AAT-encoding genes under control of the inducible LacI/T7 and XylS/Pm promoters allowed optimisation of their expression levels.

Conclusion
Engineering efforts on protein and fermentation level resulted in an E. coli strain that anaerobically produced 42.8 mM (3.8 g/L) ethyl acetate from glucose with an unprecedented efficiency, i.e. 0.48 C-mol/C-mol or 72% of the maximum pathway yield.
Predicting liver cytosol stability of small molecules
Authors: Shah Pranav , Siramshetty Vishal B. , Zakharov Alexey V. , Southall Noel T. , Xu Xin , Nguyen Dac-Trung 
Published: April 2020

AbstractOver the last few decades, chemists have become skilled at designing compounds that avoid cytochrome P (CYP) 450 mediated metabolism. Typical screening assays are performed in liver microsomal fractions and it is possible to overlook the contribution of cytosolic enzymes until much later in the drug discovery process. Few data exist on cytosolic enzyme-mediated metabolism and no reliable tools are available to chemists to help design away from such liabilities. In this study, we screened 1450 compounds for liver cytosol-mediated metabolic stability and extracted transformation rules that might help medicinal chemists in optimizing compounds with these liabilities. In vitro half-life data were collected by performingin-houseexperiments in mouse (CD-1 male) and human (mixed gender) cytosol fractions. Matched molecular pairs analysis was performed in conjunction with qualitative-structure activity relationship modeling to identify chemical structure transformations affecting cytosolic stability. The transformation rules were prospectively validated on the test set. In addition, selected rules were validated on a diverse chemical library and the resulting pairs were experimentally tested to confirm whether the identified transformations could be generalized. The validation results, comprising nearly 250 library compounds and corresponding half-life data, are made publicly available. The datasets were also used to generate in silico classification models, based on different molecular descriptors and machine learning methods, to predict cytosol-mediated liabilities. To the best of our knowledge, this is the first systematic in silico effort to address cytosolic enzyme-mediated liabilities.