bluuebunny/crossref_metadata_2025_split huggingface dataset:
Blood Culture Evaluation for Carbapenem Exposure in Pyogenic Liver Abscess: A Two-Center Retrospective Study
Authors: He Shuangjun , Yu Jie , Wang Hairong , Wang Lifeng , Chen Yi , Zhou Wei 
Published: November 2020

Abstract
Background: High antibiotic consumption in pyogenic liver abscess (PLA) is unnecessary. Using the databases of two centers, we retrospectively evaluated the effects of blood culture on the exposure to carbapenems in patients with PLA.Methods: All patients diagnosed with PLA at two comprehensive tertiary care centers from 2014 to 2020 were assessed. Demographics and clinical data were analyzed, and multiple regression analysis was performed to investigate the association between blood culture and exposure to carbapenems after controlling for potential confounders.Results: Blood culture results were available in 110 (46.0%) patients, of whom 44 (40.0%) patients were tested positive on bacterial culture. The blood culture positivity rate was significantly higher in the sepsis subgroup than in the non-sepsis subgroup (58.1% vs. 32.9%, P = 0.015). The number of patients receiving carbapenems in the blood culture cohort was significantly lower than that in the other cohort (19.1% vs. 31.8%, P = 0.026). Multivariate analysis showed that patients in the blood culture group were less likely to be treated with carbapenems for >5 days than those in the unavailable blood culture group (adjusted OR = 0.33, 95% CI: 0.16–0.68, P=0.003), especially those in the sepsis subgroup (adjusted OR = 0.17, 95% CI: 0.05–0.53, P = 0.002). Conclusion: Blood culture might be associated with less carbapenem exposure, particularly in PLA complicated with sepsis. More attention should be paid to early blood cultures in the emergency setting.
Using Logic Model and Key Performance Indicators to Construct A Field Hospital for Coronavirus Pandemic Cases
Authors: Ali Alshehri , Owied Al Moteri Modi , Virginia Plummer , Ruth Endacott , Abdulellah Al Thobaity 
Published: November 2020

Abstract
Background. Field hospitals have been established as part of the COVID-19 pandemic response in a range of countries including the Kingdom of Saudi Arabia. Assess input, monitor activities and track output can be used to identify whether field hospitals are meeting their intended targets. Method. Based on a case study approach, the reported results include field hospital construction project performance outcomes, a description of the design process and the development and application of key performance indicators to assess field hospital efficacy.Result. Each of the logic model component (i.e. input, activities and output) has tailored indicators that address different aspects of field hospital; from the infrastructure work to the wider impacts on services.Conclusion. The logic model components and related KPIs can be extremely important in terms of providing insights into how to achieve the field hospital’s objectives.
Predicting the levels of orcinol glucoside during the treatment of osteoporosis by network pharmacology and molecular docking
Authors: liu xia , Huang Mingchun , Yang Chen , Wang Qin , Zhang Mei 
Published: November 2020

Abstract
Introduction: As a traditional Chinese medicine (TCM), Curculigo orchioides Gaertn. (Xianmao) has been widely used to treat bone-related diseases. However, the active components of this TCM, and the specific mechanisms by which it exerts effect, have yet to be elucidated. To identify potential targets for orcinol glucoside (OG), an active constituent of C. orchioides, during the treatment of osteoporosis (OP) by adopting a network pharmacology approach. Methods: First, we mined the Similarity ensemble approach (SEA), SwissTargetPrediction, DisGeNET, and Genecards databases were mined for data related to the prediction of OG- and OP-related targets. Next, we identified the common targets for OG and OP, and then used STRING software to create a protein-protein interaction (PPI) network. Then, we used topological analysis to identify which of the common targets were most significant. Then, we used the common significant targets and g:profiler to perform gene ontology (GO) term and Kyoto Encyclopedia of Genes and Genomes ( KEGG) pathway enrichment analysis. Finally, we used molecular docking to predict the targets of OG that were most relevant to the treatment of OP and investigated the potential pharmacological mechanisms that might be involved. Results: In total, 130 potential targets of OG, and 4582 targets relevant to OP, were subjected to network analysis. There were 73 common targets; these identified the principal pathways linked to OP. In addition, topological analysis identified 14 key targets. Most of the predicted targets played crucial roles in the PI3K-AKT signaling pathway. Molecular docking identified ten core targets (VEGFA, IL6, EGFR, MAPK1, HRAS, CCND1, FGF2, IL2, MCL1 and CDK4), thus indicating that OG may promote osteoblast proliferation and differentiation by accelerating progression of the cell cycle.Conclusions: This research provides a theoretical base for identifying the specific potential mechanisms of OG in treatment of OP.
Oleanolic acid inhibits the proliferation of Hela cells in cervical cancer by regulating the ACSL4 ferroptosis signaling pathway
Authors: Jiang Xiaofei , Shi Mingqing , Sui Miao , Yuan Yizhen , Zhang Shuang , Xia Qinhua , Zhao Kai 
Published: November 2020

Abstract
Background: Cervical cancer continues to be the leading cause of cancer deaths among women worldwide. Oleanolic acid (OA) is a naturally occurring substance found in the leaves, fruits, and rhizomes of plants that has anti-cancer activity. Methods: We used tumor-bearing mice as the animal model and Hela cell as cell models. Western blot was used for detecting the expression of proteins in ferroptosis related proteins acyl-CoA synthase long-chain family member 4 (ACSL4), ferritin heavy chain (FTH1), transferrin receptor (TfR1) and glutathione peroxidase 4 (GPX4) in vivo and in vitro. MTT and EdU was for the detection of the viability of Hela cells. Results: In vivo experiments showed that OA significantly reduced the size and mass of cervical cancer tumors. In vitro experiments showed that OA significantly reduced the viability and proliferation capacity of Hela cells. In both in vivo and in vitro assays, OA increased the level of oxidative stress and Fe2+ content, and increased the expression of ferroptosis related proteins. We found high expression of ACSL4 in both xenograft models and cervical carcinoma cells. Meanwhile, knockdown of ACSL4 expression using shRNA in cervical cancer cells significantly increased cell viability and proliferation. In addition, decreased ROS levels and GPX4 were detected in ACSL4 knockdown cervical cancer cells, suggesting that ACSL4 inhibition may contribute to the reduction of ferroptosis within Hela cells and thus improve Hela cell survival. Conclusion: Promotion of ACSL4 dependent ferroptosis through OA may be an effective approach to treat cervical cancer.
Application of Personalized Three-Dimensional Printing for Shoulder Joint Prosthesis in the Treatment of Shoulder Joint Tuberculosis: A Case Report
Authors: Han Yanlong , Kaken Habaxi , Zhao Wei , Damaola Ainiwaerjiang , Wang Li 
Published: November 2020

Abstract
Background: This article presents an application of the use of a personalized three-dimensional (3D)-printed reverse shoulder prosthesis to be used in a reverse total shoulder arthroplasty (RTSA) for a patient with tuberculosis of the shoulder joint. Background: Patient concerns: A 37-year-old female, who suffered a trauma on her left shoulder 17 years ago, reported significant worsening pain and limited physical activity within the last 6 months. The left shoulder joint activity was clearly restricted. Diagnosis: Her clinical manifestations, bone changes in shoulder joint, and imaging results were all consistent with extended exposure to tuberculosis of the shoulder joint. Interventions: For this patient, a personalized 3D-printed titanium alloy shoulder joint prosthesis was used in the RTSA. The patient’s stability was regularly monitored and followed up after operation. During pre-operation, post-operation, and follow-up, the scores of ASES, Neer Shoulder score, and Constant-Murley Shoulder were evaluated. Results: 3D shoulder prosthesis was successfully implanted into the patient and complete anatomic reconstruction was performed intraoperatively. ASES, Neer, and Constant-Murley scores were gradually improved on follow-up. The X-ray image showed good bone healing and no changes in the position of the prosthesis seven and a half years post-operation. The functionality of the shoulder joint was well restored and no restrictions were found in activities of daily living. Conclusion: Personalized 3D-printed shoulder joint prosthesis is very effective in the treatment of advanced tuberculosis of the shoulder joint. 3D-printed technology may be a novel and efficient method for similar arthroplasties and severe bone defects.
Analysis the Curative Effect of One-Third Steel Plate Applied in the Medial Column of Pilon Fracture
Authors: Wang Zhuo 
Published: November 2020

Abstract
Objective: To analysis the difference of efficacy between the one-third tube steel plate and screw on the medial column of Ruedi-Augower III pilon fracture.Methods: The clinical data of 26 patients with Ruedi-Augower type III pilon fractures admitted from April 2016 to April 2019 were collected. Patients were divided into two groups according to the reconstruction materials. In the control group, 16 cases were fixed with screws on the medial column. In the observation group, 10 cases were reconstructed with a one-third tube steel plate on the medial column. The reduction quality was evaluated by using Burwell–Charnley’s radiographic criteria. Ankle function was evaluated by using the American Orthopedic Foot and Ankle Society (AOFAS) ankle-hindfoot score. The preoperative swelling time, operation time, intraoperative blood loss, fracture healing time, postoperative weight-bearing time, postoperative AOFAS ankle-hindfoot score and postoperative Burwell-Charley Radiology Score were compared between the two groups. Results: In the all patients, nobody developed deep tissue infections. The fracture healing time was 15.07±0.93 weeks, and the weight-bearing time after the operation was 6.91±0.58 weeks. The satisfactory rate was 90.00% according to Burwell–Charnley’s reduction criteria and the second month postoperative AOFAS ankle-hindfoot score was 66.30±2.79.As for the control group, the fracture healing time was 15.83±0.56 weeks, and the postoperative weight-bearing time was 8.60±0.50 weeks. Only 70% fractures accomplished anatomic reduction on the basis of Burwell–Charnley’s reduction criteria and the AOFAS ankle-hindfoot score only got 75.80±1.94 in the second month after surgery.Conclusion: When Ruedi-Augower type III pilon fractures were treated with one-third tube steel plate in the medial column, compared with the simple screw fixation, it took into account the excellent healing of soft tissues meanwhile it promised the weight-bearing time earlier and less loss rate of long-term postoperative reduction.
Identification of Chromatin Accessibility During The Early Stage of Pig SCNT Embryo Reprogramming by ATAC-Seq
Authors: Zhang Xue , Li Juan , Tao Ruixin , Jiang Aiwen , Zhou Changyin , Xu Hongxia , Li Weijian , Meng Xueqing , Zhang Liangliang , Zhang Xuan , Wu Wangjun , Tao Jingli , Liu Honglin 
Published: November 2020

Abstract
Background: Somatic cell nuclear transplantation (SCNT) can transform highly differentiated donor nuclei into pluripotent nuclei through the large-scale reprogramming of chromatin. The reprogramming of chromatin has been documented to take place in the first few hours after SCNT embryo activation. Thus, studies that characterize dynamic changes in chromatin during the first few hours after embryo activation could provide insight into the mechanism and significance of genome-wide reprogramming. However, few studies have examined the epigenetic remodeling of reconstructed embryos during the early stage of reprogramming.Results: We conducted ATAC-seq on 50 porcine SCNT-HMC embryos and 50 parthenogenetic activation (PA) embryos 10 h after activation. Along with pig embryonic fibroblast (PEF) ATAC-seq data, we found low levels of chromatin accessibility and gene transcription in SCNT and PA embryos. Moreover, PEF genes and the X chromosome became inaccessible during embryo reprogramming. GO enrichment analysis revealed that the molecular functions related to accessible chromatin in embryos primarily included transcriptional regulatory activity and SMAD binding. The differentially accessible chromatin sites between SCNT and PEF were primarily related to transcriptional activity and histone modification.Conclusions: Despite the tight chromatin structure during the early stage of embryo reprogramming, some accessible chromatin sites, which were primarily distributed in the intergenic region, were still detected. Dynamic changes in chromatin accessibility during reprogramming were primarily related to transcriptional activity and histone modification. Generally, this study provided new insight into the dynamics and importance of chromatin accessibility during the early stages of embryo reprogramming.
Unexpected Echocardiographic Findings: Accessory Mitral Valve Tissue in Adults
Authors: Rao Ying , Chen Wei , Wang Yu , Song wenjuan 
Published: November 2020

Abstract
Background: Accessory mitral valve tissue (AMVT) is a rare congenital cardiac anomaly, which is associated with other congenital heart diseases. It is diagnosed in neonates or childhood and rarely in adulthood. Nevertheless, AMVT is an incidental finding or described as isolated. Echocardiography, especially three-dimensional(3D) echocardiography is considered as an optimal imaging technique for AMVT diagnosis.Case presentation: We herein presented the two asymptomatic adult cases with AMVT, who presented with varying degrees of symptomatic left ventricular outflow tract (LVOT) obstruction. One presented with mild LVOT obstruction and no surgery was required, and another one with significant LVOT obstruction was recommended for surgical excision.Conclusions: We emphasized the usefulness of echocardiography in the morphology detection of AMVT, and the importance of operation guidance and follow-up.
Comparison and Analysis of kW-Class SOFC-CHP Systems with Different Fuels
Authors: Zhu Jianzhong , Meng Hao , Han Minfang 
Published: November 2020

Abstract
SOFC is an ideal device for developing distributed combined heat and power (CHP) systems. In this paper, kW-class SOFC system models based on semi-empirical SOFC stack model were established to optimize the systems with the aim of maximizing the system electrical efficiency. Four types of SOFC-CHP systems using different fuel including natural gas (NG), hydrogen, methanol, syngas were compared, and the relationships between key parameters and system energy effciency were analyzed. Simlulation results show that decreasing the air inlet temperature (AIT) and increasing stack temperature will promote electrical effciency most significantly. In addition, the operational costs of four different systems were also compared, and among which the methanol-fueled system was the lowest.
First Experience of Standard Linac, Rapid Arc and Dose-drop Scheme Based Total Body Irradiation
Authors: Xu Zhiyuan , Yang Li , Hui Tim , Jiang Xiaoqin , chan Jeff , Cheung Jacob , Wang Qian , Lee Eric , Zhou Cheng , Cai Linbo , Chen Longhua 
Published: November 2020

Abstract
Background and purpose: To introduce a standard linac with true attachment free, rapid arc and dose-drop scheme for positioning related dose deviation control based total body irradiation(TBI).Materials/Methods: One eight years old girl diagnosed with acute lymphocytic leukemia underwent TBI in 2020. Target volumes and organs at risk were contoured after CT simulation. Total sixteen ARC and four AP-PA from five isocenters were designed. A dose-drop scheme on both sides of adjacent region were performed to reduce positioning-related dose deviation. A series of quality assurance before radiotherapy and real-time dose monitoring during radiotherapy were carried out.Results: The average on board imaging (OBI) time of per fraction was 40.3 min, the average beam on time of per fraction was 37.2 min, the average time to change from head first to feet first position was 18.4 min. The average mean lung dose was 9.89 Gy, the maximum lens dose was 7.60 Gy, the mean PTV_total dose was 12.17Gy, 98.23% PTV_total volume was covered by 90% of the prescription dose. The maximum dose (Dmax) of PTV_total was 13.65Gy. Dmean and V10.8 of PTV_total are only slightly different(0.49% - 1.89% and 0.26% - 1.04% respectively) even with an error of 5 - 20mm longitudinal misalignments. Gamma passing rate(3mm/ 3% Gamma criteria) are between 93.5% and 100%. Real-time dose monitoring showed an overall deviation of -3.9%±5.51%.Conclusions: Standard linac, rapid arc and dose-drop for positioning-related dose deviation control based total body irradiation is feasible, accurate, and reliable. It is worthy of clinical application.
Estimation of Polycyclic Aromatic Hydrocarbons pollution, risk assessment and loads into Mediterranean Sea from Volturno River, Southern Italy.
Authors: Montuori Paolo , Rosa Elvira De , Duca Fabiana Di , Provvisiero Donatella Paola , Sarnacchiaro Pasquale , Nardone Antonio , Triassi Maria 
Published: November 2020

Abstract
Background: This study reports the data on the contamination caused by polycyclic aromatic hydrocarbons (PAHs) drained into the Volturno River and its environmental impact on the Tyrrhenian Sea (Central Mediterranean Sea). One of the key aims of this study is to use the PAHs as indicators of pollution, by identifying the main sources from which these pollutants originate. Also, the ecosystem health risk and the seasonal and spatial distribution of PAHs in samples of water and sediment was assessed. The 16 PAHs identified by the USEPA as priority pollutants were determined in the water dissolved phase, suspended particulate matter and sediments collected from 10 sites in four seasons.Results: A multidimensional statistical approach was used to identify three pollution composite indicators. Contaminant discharges of PAHs into the sea were calculated in about 3.158,2 kg/year showing that this river should account as one of the main contribution sources of PAHs to the Tyrrhenian Sea. Total concentrations of PAHs varied in ranges 434.8 to 872.1 ng g-1 and 256.7 to 1686.3 ng L-1 in sediment samples and in water (total of water dissolved phase and suspended particulate matter), respectively. Conclusion: The statistical results indicated that the PAHs mainly had a pyrolytic source and the rainy season was the most polluted time. The toxic equivalent concentration (TEQ) of carcinogenic PAHs was 130.3 to 302.1 ngTEQ g-1, implying that the Volturno River basin presents a definite carcinogenic risk.
Paclitaxel Resistance in Ovarian Cancers Relies on a PGAM1 Mediated Glycolytic Metabolism
Authors: Feng Yan , Zhang Xu , Zhang Songfa , Xu Shanshan , Chen Xiaojing , Zhou Caiyun , Wang Xinyu , Xie Xing , Lu Weiguo 
Published: November 2020

Abstract
Background: Paclitaxel, as an anti-microtubule drug, has been recommended to be the first-line chemotherapy agent for ovarian cancer for about two decades. However, most patients with advanced stage relapse within two years and ultimately die of relapsed cancer displaying increased chemoresistance. The mechanisms underlying paclitaxel resistance remain ncompletely understood.Methods: The paclitaxel-resistant ovarian cancer cell line SKOV3-TR30 and its parental cell line SKOV3 were analyzed by proteinomics. The glycolytic enzyme PGAM1-modulated glycolytic flux including pyruvic acid production and lactic acid production, and mitochondrial function was investigated using the assays kit. The correlations between PGAM1 expression, overall survival and progression free survival were evaluated for ovarian cancer patients. To elucidate the underlying mechanisms involved in paclitaxel resistance, PGAM1 gene knockout, gene overexpression and/or manipulation of glycolytic products were employed, followed by Western blot, immunostaining, and cell viability assay.Results: PGAM1 was highly expressed in the paclitaxel resistant ovarian cancer cell line SKOV3-TR30, as compared to its parental cell line SKOV3. A high expression of PGAM1 was ignificantly correlated with a reduced overall survival and progression free survival in ovarian cancer patients. Under paclitaxel exposure, SKOV3 cells showed a stronger mitochondrial function than SKOV3-TR30 cells, suggesting that under the stress, SKOV3-TR30 cells had responded with enhanced glycolysis rather than undergoing oxidative phosphorylation. Down-regulation of PGAM1 in SKOV3 TR30 cells resulted in decreased paclitaxel resistance. Up-regulation of PGAM1 in SKOV3 cells led to enhanced paclitaxel resistance. Analysis of the lycolytic flux revealed that PGAM1-mediated pyruvic acid or lactic acid production could control the capabilities of ovarian cancer cell resistance to paclitaxel.Conclusions: Our study demonstrated that PGAM1 could act as a modulator linked to paclitaxel sensitivity in ovarian cancer cells. Blocking PGAM1 may provide a new approach to reverse paclitaxel resistance in ovarian cancer patients.
The Psychological Responses and Associated Factors of Chinese College Students During COVID-19 Outbreak in February
Authors: Zhang Xu-dong , Jing Hui-quan , Wang Yang , Li Kun-hang , Zhao Dan , Yu Hong-ye , Zhang Lu-yang , Wang Wei , Liu Yan , Song Yue-qun , Zhang Feng , Xu Ming-lu , Sun Xiao-wei , Bao Yi-jun 
Published: November 2020

Abstract
BackgroundThe novel coronavirus pneumonia (COVID-19) is a highly contagious and highly pathogenic disease caused by a novel coronavirus（SARS-CoV-2）and has become pandemic within a short period of time. The epidemic has brought not only the risk of death from infection but also unbearable psychological pressure. College students as a special group, their mental health status need to be studied during the outbreak of COVID-19.MethodsWe used the Self-Rating Anxiety Scale (SAS), Self-Rating Depression Scale (SDS), and the compulsive behavior part of the Yale-Brown Obsessive-Compulsive Scale (YBOCS), combined with demographic information, using online questionnaires to research, and the study was conducted between February 21 and 24, 2020. A total of 2270 valid questionnaires were collected, the respondents of these questionnaires included 563 medical students and 1707 non-medical students. We separately analyzed the mental health status of medical and non-medical students during the outbreak of COVID-19.ResultsOf the 563 medical students, 20 (3.55%) students had anxiety symptoms, and 57 (10.12%) students had depressive symptoms. Gender, PMH, compulsive behavior, and regularity of daily life during the epidemic outbreak were correlated with their anxiety symptoms and age, PMH, compulsive behavior, and regularity of daily life during the epidemic outbreak were associated with their depressive symptoms. Of the 1707 non-medical students, 66 (3.87%) students had anxiety symptoms, and 180 (10.54%) students had depressive symptoms. Gender, contact history of similar infectious disease, PMH, compulsive behavior, regularity of daily life and exercise during the epidemic outbreak and concern on COVID-19 were correlated with their anxiety symptoms and contact history of similar infectious disease, PMH, compulsive behavior, regularity of daily life and exercise during the epidemic outbreak and concern on COVID-19 were associated with their depressive symptoms.ConclusionsResults indicated that gender, age, contact history of similar infectious disease, past medical history (PMH), compulsive behavior, regularity of daily life, and exercise during the epidemic outbreak are the key factors making college students anxious or depressed. The results provided a theoretical basis for relevant interventions; it is also essential for medical education and public health epidemic prevention.