bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bacterial Composition and Diversity Associated with Rhizosphere of the Chinese Medicinal Herb Dendrobium
Authors: Yuan Yingdan , Zu Mengting , Liu Lei , Song Xiaomei 
Published: September 2020

Abstract
Background: Dendrobium is a precious herbal belongs to Orchid and widely used as health care traditional Chinese medicine in Asia. Although orchids are mycorrhizal plants, most researches still focus on endophytes, and there is still large unknown in rhizosphere microorganisms. In order to investigate the rhizosphere microbial community of different Dendrobium species during the maturity stage, we used high-throughput sequencing to analyze microbial community in rhizosphere soil during maturity stage of three kinds of Dendrobium species.Results: In our study, a total of 240,320 sequences and 11,179 OTUs were obtained from these three Dendrobium species. According to the analysis of OTU annotation results, different Dendrobium rhizosphere soil bacteria include 2 kingdoms, 63 phyla, 72 classes, 159 orders, 309 families, 850 genera and 663 species. Among all sequences, the dominant bacterial phyla (relative abundance > 1%) were Proteobacteria, Actinobacteria, Bacteroidetes, Acidobacteria, Firmicutes, Verrucomicrobia, Planctomycetes, Chloroflexi, Gemmatimonadetes. We analyzed the environmental factors of the growth of Dendrobium and found that the environmental factor that affects the rhizosphere soil microorganisms of Dendrobium is the soil factor. Among them, soil factors most closely related to the influence of Dendrobium rhizosphere soil microorganisms include total nitrogen, available phosphorus, ammonium nitrogen and pH value.Conclusions: We found that the rhizosphere bacterial communities of the three kinds of Dendrobium have significant differences, and the main species of rhizosphere microorganisms of Dendrobium are concentrated in the Proteobacteria, Actinobacteria, Bacteroidetes. Moreover, the smaller the level of bacterial, the greater the difference among Dendrobium species. Soil is the most important environmental factor affecting the bacterial communities in the rhizosphere soil of Dendrobium. These results fill the gap in the rhizosphere microbial community of Dendrobium and provide a theoretical basis for the subsequent mining of microbial functions and the study of biological fertilizers.
Height and Volume Restoration In Osteoporotic Vertebral Compression Fractures: A Biomechanical Comparison Of Standard Balloon Kyphoplasty Versus Tektona In A Cadaveric Fracture Model
Authors: Krüger Antonio , Bäumlein Martin , Knauf Tom , Pascal-Moussellard Hugues , Ruchholtz Steffen , Oberkircher Ludwig 
Published: September 2020

Abstract
Background: Standard balloon kyphoplasty represents a well-established treatment option for osteoporotic vertebral compression fractures. Aim of the present study was to evaluate two different methods of percutaneous augmentation (standard Balloon kyphoplasty (BKP) versus Tektona (TEK)) with respect to height restoration. Methods: Four-teen vertebral bodies of two female cadavers were examined. Fractures were created using a standardized protocol. CT-scans were taken before and after fracture, as well as after treatment. Afterwards two groups were randomly assigned in a matched pair design: 7 vertebral bodies (VB) were treated with (BKP, Kyphon, Medtronic) and 7 vertebral bodies by TEK (Spineart, Switzerland) Anterior, central and posterior vertebral body heights were evaluated by CT-scans. Volumetry was performed using the CT-scans at three different timepoints. Results: Values before fracture represent 100 %. The anterior height after fracture was reduced to 75,99 (± 4,8) % for the BKP group and to 76,54 (± 9,17) % in the TEK Group. Statistically there was no difference for the groups (p = 1). After treatment the values increased to 93,06 (±5) % for the BKP Group and 87,71 (±6,2) % for the TEK Group. The difference before and after treatment was significant for both groups (BKP p =0,0006; TEK p= 0,03). Within the groups, there was no difference (p=0,13). The Volume of the vertebral body was reduced to 82,29 (±8,4) % in the BKP Group and to 76,54 (±8,6) % in the TEK Group. After treatment the volume was 89,26 (±6,9) % for the BKP Group and 88,80 (±8,7) % for the TEK Group. The difference before and after treatment was significant only for the TEK group (BKP p =0,0728 n.s.; TEK p= 0,0175). Within the groups, there was no difference (p=0,2). The average cement volume used was 6,1 (range 3,6 - 9 ml) for the BKP group and 5,3 (3 - 7,2 ml) for the TEK group respectively.Conclusions: Based on our results the new System Tektona in osteoporotic compression fractures might represent a promising alternative for the clinical setting, especially preserving bone. Further biomechanical tests and clinical studies have to proof Tektona`s capabilities.
Stages of Embryonic Development of the Amazonian turtle Kinosternon Scorpioides (Testudines, Kinosternidae)
Authors: Chaves Lianne Pollianne Fernandes Araujo , Viana Diego Carvalho , Tchaika Ligia , Caldas Juliana Maria Alves , Neto Antônio Chaves Assis , Miglino Maria Angélica , Sousa Alana Lislea 
Published: September 2020

Abstract
Background: The developmental biology of K. scorpioides is described, based on the phenotype. This species is important for the flora because they are excellent seed disseminators. In addition, basic embryological information is not yet fully clarified, and this research provides unprecedented information on the chelonian embryology of the Amazonian fauna. Methods: Females were monitored throughout the reproductive cycle, by video monitoring, to identify nests and the presence of newly laid eggs. At regular weekly intervals, embryo samples were collected fixed in a 4% paraformol solution and preserved in 70% alcohol. For the embryonic characterization we used a stereomicroscope and the scanning electron microscopy method. Results: We describe 15 embryonic stages for a 15-week (105-day) incubation cycle. Only at 42 days (6th week) was the morphological characterization of a chelonian observed and at the 12th week (Stage XII) the phenotypic characterization of the species kinosternon scorpioides. Conclusions: In view of the evidence, we found that these phases are similar to the other turtles, with structural variations in the appearance and disappearance of structures due to the specific characteristics for the species.
Understanding the Influence of Substrate When Growing Tumorspheres
Authors: Benítez Lucía , Barberis Lucas , Vellón Luciano , Condat Carlos Alberto 
Published: September 2020

Abstract
Background: Cancer stem cells are important for the development of many solid tumors. These cells receive promoting and inhibitory signals that depend on the nature of their environment (their niche) and determine cell dynamics. Mechanical stresses are crucial to the initiation and interpretation of these signals. Methods: A two-population mathematical model of tumorsphere growth is used to interpret the results of a series of experiments recently carried out in Tianjin, China, and extract information about the intraspecific and interspecific interactions between cancer stem cell and differentiated cancer cell populations. Results: The model allows us to reconstruct the time evolution of the cancer stem cell fraction, which was not directly measured. We find that, in the presence of stem cell growth factors, the interspecific cooperation between cancer stem cells and differentiated cancer cells induces a positive feedback loop that determines growth, independently of substrate hardness. In a frustrated attempt to reconstitute the stem cell niche, the number of cancer stem cells increases continuously with a reproduction rate that is enhanced by a hard substrate. For growth on soft agar, intraspecific interactions are always inhibitory, but on hard agar the interactions between stem cells are collaborative while those between differentiated cells are strongly inhibitory. Evidence also suggests that a hard substrate brings about a large fraction of asymmetric stem cell divisions. In the absence of stem cell growth factors, the barrier to differentiation is broken and overall growth is faster, even if the stem cell number is conserved. Conclusions: Our interpretation of the experimental results validates the centrality of the concept of stem cell niche when tumor growth is fueled by cancer stem cells. Niche memory is found to be responsible for the characteristic population dynamics observed in tumorspheres. A specific condition for the growth of the cancer stem cell number is also obtained.
The Efficacy of Aromatherapy With Lemon Balm (Melissa Officinalis L.) on Sleep Quality in Cardiac Patients: A Randomized Controlled Trial
Authors: Lotfi Adeleh , Mohtashami Jamileh , Khangholi Zahra Arab , Shirmohammadi-Khorram Nasrin 
Published: September 2020

Abstract
Background: The importance of sleep in physical and mental well-being is generally acknowledged by both health professionals and the general public.This study investigated the effects on inhalation aromatherapy on sleep quality in cardiac patients.Methods: Ninety-six men and women aged between 20 and 75 were randomized to inhale aromatherapy essential oil from Lemon Balm (Melissa officinalis L.) or odorless sesame oil (the placebo) for 30 minutes twice daily for 3 days. Sleep quality by Verran Synder- Halpern (VSH) scale were assessed before and after period.Results: Compared with the placebo, the experimental group showed that the components such as subjective sleep quality, sleep disturbance, and daytime dysfunction were significantly decreased (P< 0.005).Conclusion: Aromatherapy may be used as an independent nursing intervention for improving sleep quality of cardiac patients.
Prevalence and risk factors of bowel symptoms in Korean patients with ulcerative colitis in endoscopic remission: a restrospective study
Authors: Nam Kwangwoo , Park Sang Hyoung , Oh Jun Ho , Lee Ho-Su , Noh Soomin , Park Jae Cheol , Kim Jin Yong , Oh Eun Hye , Kim Jeongseok , Ham Nam Seok , Hwang Sung Wook , Yang Dong-Hoon , Ye Byong Duk , Byeon Jeong-Sik , Myung Seung-Jae , Yang Suk-Kyun 
Published: September 2020

Abstract
Background
Many patients with ulcerative colitis (UC) in clinical remission frequently complain of bowel symptoms such as increased stool frequency (SF) and rectal bleeding (RB). However, studies on these patient-reported outcomes in patients with inactive UC are limited, especially in Korea. We investigated the prevalence and risk factors of bowel symptoms in Korean patients with inactive UC.
Methods
We investigated the prevalence of bowel symptoms in patients with endoscopically quiescent UC between June 1989 and December 2016 using a well-characterized referral center-based cohort. The Mayo clinic score (MCS) was used to evaluate bowel symptoms at the most recent visit near the date of endoscopy. Clinical characteristics of the patients were compared based on the presence or absence of bowel symptoms.
Results
Overall, 741 patients with endoscopically quiescent UC were identified, of whom 222 (30%) and 48 (6.5%) had an SF and RB subscore of ≥ 1, respectively. Patients with bowel symptoms (SF + RB ≥ 1; n = 244 [32.9%]) had higher rates of left-sided colitis (E2) or extensive colitis (E3) than patients without bowel symptoms (SF + RB = 0; n = 497 [67.1%]; P = 0.002). Multivariate analysis revealed that female sex (odds ratio [OR]: 1.568; 95% confidence interval [CI]: 1.023–2.402; P = 0.039) and E2 or E3 (OR 1.411; 95% CI 1.020–1.951; P = 0.038) were the significant risk factors for increased SF.
Conclusions
This study revealed that one-third of patients with endoscopically quiescent UC reported increased SF. Female sex and disease extent may be associated with bowel symptoms.
Exosomes Derived From Human Umbilical Cord Mesenchymal Stem Cells Alleviate Acetaminophen-induced Acute Liver Failure Through Activating ERK and IGF-1R/PI3K/AKT Signaling Pathways
Authors: Wu Han-You , Zhang Xiang-Cheng , Cao Ye , Yan Kai , Li Jing-Yuan , Tao Li , Jie Zhi-Gang , liu quanwen 
Published: September 2020

Abstract
Background: Human umbilical cord mesenchymal stem cells (hUCMSCs) transplantation has been proposed as a promising therapeutic approach for treating acute liver failure (ALF), but its application is limited by immune rejection and tumor formation. Exosomes contain various bioactive cargos including mRNA, microRNA, and protein that can alter the cellular enviroment to enhance tissue repair. However, the exact effects of hUCMSCs derived exosomes (hUCMSC-Exo) on the healing of ALF and their potential mechanisms are not explored.Methods: In vivo, mouse model of ALF were set up through a single intraperitoneal injection of acetaminophen (APAP, 380 mg/kg). In vitro, human hepatocyte cells LO2 were treated with APAP (5 mM). Then APAP-induced ALF mice and APAP-injured LO2 cells were treated with hUCMSC-Exo. Finally, the effects and the mechanisms were estimated.Results: We found that a single tail vein administration of hucMSC-Exo effectively enhanced the survival rate, inhibited apoptosis in hepatocytes, and improved liver function in APAP-induced mouse model of ALF. Furthermore, the deletion of glutathione (GSH) and superoxide dismutase (SOD), generation of malondialdehyde (MDA), and the over expression of cytochrome P450 E1 (CYP2E1) and 4-hydroxynonenal (4-HNE) caused by APAP were also inhibited by hucMSC-Exo, indicating that hucMSC-Exo inhibited APAP-induced apoptosis of hepatocytes by reducing oxidative stress. Moreover, hucMSC-Exo significantly down-regulated the levels of inflammatory cytokines IL-6, IL-1β, and TNF-α in APAP-treated livers. Western blot showed that hucMSC-Exo significantly promoted the activation of ERK1/2 and IGF-1R/PI3K/AKT signaling pathways in APAP-injured LO2 cells, resulting in the inhibition of apoptosis of LO2 cells. Importantly, PI3K inhibitor LY294002 and ERK1/2 inhibitor PD98059 could reverse the function of hucMSC-Exo on APAP-injured LO2 cells in some extent. Conclusions: Our results suggest that hucMSC-Exo offer antioxidant hepatoprotection against APAP in vitro and in vivo by inhibitiing oxidative stress-induced apoptosis via upregulation of ERK1/2 and PI3K/AKT signaling pathways, suggesting that administration of hucMSC-Exo may be an alternative approach for the treatment of ALF.
Focused Ultrasound of the Heart and Lungs in Patients With Respiratory Failure: A Randomized Controlled Multicenter Trial.
Authors: Riishede Minna , Lassen Annmarie T. , Baatrup Gunnar , Pietersen Pia Iben , Jacobsen Niels , Jeschke Klaus Nielsen , Laursen Christian Borbjerg 
Published: September 2020

Abstract
Background Ultrasound is a focus oriented tool for differentiating among cardiopulmonary diseases. Its value in the hands of emergency physicians, with various ultrasound experience, remains uncertain. We tested the hypothesis that, in emergency department patients with signs of respiratory failure, a focused cardiopulmonary ultrasound along with standard clinical examination, performed by emergency physicians with various ultrasound experience would increase the proportion of patients with correct diagnoses at 4 hours after admission compared to standard clinical examination alone. Methods In this prospective multicenter superiority trial in Danish emergency departments we randomly assigned patients presenting with acute signs of respiratory failure to intervention or control in a 1:1 ratio by block randomization. Patients received a focused cardiopulmonary ultrasound examination within 4 hours from admission. Ultrasound results were unblinded for the treating emergency physician in the intervention group. Final diagnoses and treatment were determined by blinded review of the medical record after the patients´ discharge.Results From October 9, 2015 to April 5, 2017, we randomized 218 patients and included 211 in the final analyses. At 4 hours we found; no change in the proportion of patients with correct presumptive diagnoses; intervention 79·25% (95% CI 70·3-86·0), control 77·1% (95% CI 68·0-84·3), an increased proportion of correct treatment prescribed; intervention 79·3% (95% CI 70·3-86·0), control 65·7% (95% CI 56·0-74·3) and of patients who spent less than 1 day in hospital; intervention n=42 (39·6%; 25·8 38·4), control n=25 (23·8%; 16·5-33·0). No adverse events were reported. Conclusions Focused cardiopulmonary ultrasound added to standard clinical examination in patients with signs of respiratory failure had no impact on the diagnostic accuracy, but significantly increased the proportion of correct treatment prescribed and the proportion who spent less than 1 day in hospital.Trial registration https://clinicaltrials.gov/, number NCT 02550184.
MiR-1301-3p Inhibits Epithelial to Mesenchymal Transition via Targeting RhoA in Pancreatic Cancer
Authors: Zhang Xinxue , Zhao Xin , Xu Junming , Ma Jun , Liu Zhe , Kou Jiantao , Lang Ren , He Qiang 
Published: September 2020

Abstract
Background: Micro(mi)RNAs play an essential role in the epithelial-mesenchymal transition (EMT) process in human cancers. This study aimed to uncover the regulatory mechanism of miR-1301-3p on EMT in pancreatic cancer (PC).Methods: GEO database (GSE31568, GSE41372, and GSE32688) and the PC cohort of The Cancer Genome Atlas were applied to discover the expression and prognostic role of miR-1301-3p. In the validation cohort, qRT-PCR was performed in 72 paired PC tissue samples. CCK-8, wound healing, and transwell migration assays were used to detect miR-1301-3p function on PC cells. Luciferase reporter assays and western blotting were performed to discover the potential target of miR-1301-3p on EMT.Results: Our study revealed that miR-1301-3p was downregulated in PC tissues compared with normal samples. A low level of miR-1301-3p was associated with malignant pathological differentiation, lymphatic metastasis, tumor residual, and unsatisfactory overall survival. Gene Ontology analyses indicated that miR-1301-3p possibly regulated cell cycle and adheren junction. In vitro assays showed that miR-1301-3p suppressed proliferation, migration, and invasion ability of PC cells. Mechanically, miR-1301-3p inhibits RhoA expression, and knockdown of RhoA upregulated E-cadherin; however, downregulated N-cadherin and vimentin level.Conclusions: MiR-1301-3p acts as a prognostic biomarker for PC and inhibits PC progression by targeting RhoA induced EMT process.
Cardiovascular Risk Profile in Dupuytren’s Disease: A Systematic Review and Meta-Analysis.
Authors: Mathieu Sylvain , Pereira Bruno , Dutheil Frederic , Soubrier Martin 
Published: September 2020

Abstract
Background. Patients with Dupuytren’s disease (DD) may have a higher cardiovascular (CV) risk because of association with diseases with a higher recognized CV risk, such as diabetes. However, DD is not always linked to these diseases; therefore, it seems relevant to assess the CV profile in DD.Methods. We performed a systematic literature review up to April 2020. Differences between DD patients and controls were expressed as standardized mean differences using the inverse-variance method or as odds ratios (ORs) using the Mantel-Haenszel method. Results. We obtained 51 references corresponding to 112,900 DD patients and 2,383,177 controls. We found a higher risk of death (OR=1.72 [95%CI:1.37-2.16]) and CV death (118/974 DD patients vs. 372/3948 controls; OR=1.33 [95%CI:1.07-1.66]) among DD patients compared to controls. DD patients were more often diabetic (OR=3.44 [95% CI:2.69-4.38]). In 17 studies of the general population, the incidence of diabetes was 17.5% among DD patients (11.7-24.2%). DD patients were older, and more often men or alcohol drinkers. The percentage of smokers and levels of blood pressure or total cholesterol were not different among DD patients and controls. No difference was found in triglycerides, but the risk of obesity was significantly lower in DD patients.Conclusions. We found a higher CV risk in DD but not a higher prevalence of CV risk factors, except diabetes. This may be due to the predominance of men or greater number of alcohol drinkers or diabetics. Management of CV risk is important in patients with DD, including research on alcohol consumption or diabetes.
Spontaneous Conception Following Laparoscopic Surgery in Women with Infertility at The Yaoundé Gyneco-Obstetric and Pediatric Hospital: A Cross-Sectional and Analytical Study
Authors: Fopoussi Marie Noelle Mokam , Ndoua Cyrille Claude Noa , Djuikwo Felicite , Sandie Arsène Brunelle , Ojong Samuel , Foumane Pascal 
Published: September 2020

Abstract
Background : The infertility is a common health issue affecting many couples with a broad consequence on their lives. This study investigated the effectiveness of laparoscopic surgery for treating infertility in Cameroon.Methods : We conducted a cross-sectional, analytic study in order to determine the rate of conception and associated factors following laparoscopic surgery in women who presented with infertility, and treated at the Yaoundé Gynaeco-Obstetric and Pediatric Hospital from January 1st 2007 to December 31st 2017.Results : The average age of the included patients was 32:2 4:9 years old. Married women and those with tertiary level studies were the most represented. The average duration of infertility stood at 4:6 2:8 years. The most common surgical procedures were adhesiolysis (86.1%) and neo-salpingotomy (55.4%). The conception rate after the endoscopic surgery was 39.3% (119/303). The associated factors were normal body mass index and the antecedent of two or more alive children.Conclusions : The study revealed the efficiency of laparoscopic surgery in the management of infertility.
Anti-inflammatory Property of the Essential Oil From Cinnamomum Camphora (Linn.) Presl Leaves and the Evaluation of Its Underlying Mechanism by Using Metabolomics Analysis
Authors: Chen Jiali , Tang Cailin , Zhou Yang , Zhang Rongfei , Ye Shaoxia , Zhao Zhimin , Lin Ligen , Yang Depo 
Published: September 2020

Abstract

Background: Cinnamomum camphora (Linn.) Presl has been widely used in traditional Chinese medicine for a variety of purposes. Our previous study indicated the antibacterial mechanism of the essential oil (EO) from C. camphora; however, the anti-inflammatory activity of EO and its underlying mechanism have not been clearly demonstrated. The present study aims to evaluate the anti-inflammatory principle and mechanism of EO.Methods: The anti-inflammatory activity of EO was evaluated in lipopolysaccharide (LPS)-induced BV2 microglial cells. Nitric oxide (NO) production was measured by NO assay kit. The mRNA expression levels of inducible NO synthase (iNOS), interleukin-6 (IL-6), IL-18 and IL-1β were examined by real time-PCR (RT-PCR). The secretion of pro-inflammatory cytokines in cell supernatants, including IL-6, IL-18 and IL-1β, were assessed by ELISA kits. Furthermore, the metabolic profile of BV2 microglial cells treated with or without EO was explored by GC-MS-based metabolomics analysis. Phosphofructokinase (PFK) and pyruvate kinase (PK) activities were detected by commercial kits.Results: EO significantly decreased the release of NO and the mRNA expression of iNOS in LPS-induced BV2 microglial cells. EO also attenuated LPS-induced increase in the mRNA expression and secretion of inflammatory cytokines including IL-6, IL-18 and IL-1β. 39 metabolites were identified with significantly different contents, including 21 upregulated and 18 downregulated ones, in the metabolomics analysis. Five pathways were enriched by shared differential metabolites. Additionally, compared with the control group, the glucose level was decreased, while the lactate level was increased, in the culture supernatant of LPS-induced BV2 microglia cells, which were reversed by EO treatment. Besides, compared to the LPS-treated group, the activities for PK and PFK in EO group were decreased by 17.59% and 18.23%, respectively.Conclusions: The EO from C. camphora acts as an anti-inflammatory agent, which might be mediated through attenuating the glycolysis capacity of microglial cells.