bluuebunny/crossref_metadata_2025_split huggingface dataset:
Anaplastic thyroid carcinoma with chondrosarcomatous differentiation: a case report
Authors: Liu Jixuan , Cui Ni , Ding Wenjia , Dong Xinjie , Chen Xiaoshuai , Jiang Jun , Liu Yafang 
Published: July 2020

Abstract
Background
Anaplastic thyroid carcinoma (ATC) is a rare malignant tumor. In addition to the main ATC type with classical histopathological features, the other morphological types of ATC include paucicellular variant, angiomatoid, lymphoepithelioma-like, and small-cell variant. However, an ATC variant with a chondrosarcomatous component has not been reported to date.

Case presentation
Computed tomography imaging of a 63-year-old male with a 2-month history of a cervical mass revealed a 4.5-cm lesion with heterogeneous enhancement in the left thyroid lobe and two smooth and homogeneous nodules in the right thyroid lobe. The patient underwent total thyroidectomy and cervical lymph node resection. Histologically, the tumor boundary in the left lobe was clear, with a few mitotically active, spindle sarcoma-like tumor cells observed in some areas. Immunohistochemically, these spindle cells were positive for vimentin and negative for cytokeratin, paired box-8, epithelial membrane antigen, calcitonin, thyroglobulin, and thyroid transcription factor-1. In other areas, abundant cartilage matrix production and irregularly shaped lobules of cartilage, often separated by fibrous bands, were observed. The chondrocytes appeared mildly/moderately atypical and contained enlarged, hyperchromatic nucleoli. One of the two nodules in the right thyroid lobe had a clear boundary and comprised some bland spindle cells in a prominently collagenous stroma with clear boundaries. The other nodule in the right thyroid lobe was completely enclosed within a thin, fibrous capsule and exhibited normofollicular and microfollicular architecture. The patient received adjuvant radiotherapy after the surgery and was free of any local or regional recurrence or distant metastases at the 8-month follow-up evaluation.

Conclusions
This unusual case of ATC with chondrosarcomatous differentiation is an important addition to the morphology spectrum of ATC types.
Ovarian mesonephric-like adenocarcinoma arising in serous borderline tumor: a case report with complex morphological and molecular analysis
Authors: Dundr Pavel , Gregová Mária , Němejcová Kristýna , Bártů Michaela , Hájková Nikola , Hojný Jan , Stružinská Ivana , Fischerová Daniela 
Published: July 2020

Abstract
Background
Mesonephric-like adenocarcinoma (M-LAC) is a rare, recently described tumor occurring in the uterine corpus and ovary, which shares the same morphological and immunohistochemical features with the more common mesonephric adenocarcinoma (MAC), which mostly arises the uterine cervix. Despite the similarities between these tumors, the histogenesis of M-LAC is still disputable.

Case presentation
Sixty-one-year-old woman presented with an advanced tumor of the left ovary with intraabdominal spread and liver metastases. After receiving 5 cycles of neoadjuvant chemotherapy, she underwent a hysterectomy with bilateral salpingo-oophorectomy, and resection of the liver metastasis, omentum, and appendix. Histologically, the ovarian tumor consisted of two components, whose morphology and immunohistochemical results were typical of either a serous borderline tumor (immunohistochemical positivity for PAX8, WT1, ER and PR) or a mesonephric-like carcinoma (immunohistochemical positivity for PAX8, TTF1 and GATA3). Only the component of the mesonephric-like adenocarcinoma metastasized to the omentum and liver. A molecular analysis with a panel of 271 genes (size 1020 kbp) was performed separately on samples from the borderline tumor, primary ovarian mesonephric-like adenocarcinoma, and liver metastasis. The results showed the clonal origin of all samples, which shared the same KRAS (NM_004985.3:c.34G > T, p.(G12C)) and PIK3CA (NM_006218.2:c.1633G > A, p.(E545K)) somatic mutations. Moreover, in the sample from the primary mesonephric-like carcinoma and its liver metastasis a likely pathogenic somatic MYCN mutation (NM_005378.4:c.131C > T, p.(P44L) was found. In all samples, the deletion of exons 9–10 in the CHEK2 gene was present, which is in concordance with the previously performed genetic testing of the blood specimen which revealed the hereditary CHEK2 mutation in this patient.

Conclusions
Our result support the theory that at least some mesonephric-like ovarian adenocarcinomas are of Müllerian origin. The serous borderline tumor seems to be a precursor of mesonephric-like adenocarcinoma, which has been proven in our case by both tumors sharing the same mutations, and the presence of cumulative molecular aberrations in the mesonephric-like adenocarcinoma.
The application of histone deacetylases inhibitors in glioblastoma
Authors: Chen Rui , Zhang Mengxian , Zhou Yangmei , Guo Wenjing , Yi Ming , Zhang Ziyan , Ding Yanpeng , Wang Yali 
Published: July 2020

AbstractThe epigenetic abnormality is generally accepted as the key to cancer initiation. Epigenetics that ensure the somatic inheritance of differentiated state is defined as a crucial factor influencing malignant phenotype without altering genotype. Histone modification is one such alteration playing an essential role in tumor formation, progression, and resistance to treatment. Notably, changes in histone acetylation have been strongly linked to gene expression, cell cycle, and carcinogenesis. The balance of two types of enzyme, histone acetyltransferases (HATs) and histone deacetylases (HDACs), determines the stage of histone acetylation and then the architecture of chromatin. Changes in chromatin structure result in transcriptional dysregulation of genes that are involved in cell-cycle progression, differentiation, apoptosis, and so on. Recently, HDAC inhibitors (HDACis) are identified as novel agents to keep this balance, leading to numerous researches on it for more effective strategies against cancers, including glioblastoma (GBM). This review elaborated influences on gene expression and tumorigenesis by acetylation and the antitumor mechanism of HDACis. Besdes, we outlined the preclinical and clinical advancement of HDACis in GBM as monotherapies and combination therapies.
Blocking IL-1 to prevent respiratory failure in COVID-19
Authors: van de Veerdonk Frank L. , Netea Mihai G. 
Published: July 2020

AbstractCOVID-19 is an emerging disease that can manifest itself as asymptomatic or mild respiratory tract infection in the majority of individuals, but in some, it can progress into severe pneumonia and acute respiratory distress syndrome (ARDS). Inflammation is known to play a crucial role in the pathogenesis of severe infections and ARDS and evidence is emerging that the IL-1/IL-6 pathway is highly upregulated in patients with severe disease. These findings open new avenues for host-directed therapies in patients with symptomatic SARS-CoV-2 infection and might in addition to antiviral treatment be enough to curb the currently unacceptably high morbidity and mortality associated with COVID-19.
Resting behavior of Aedes aegypti in southeastern Senegal
Authors: Diallo Diawo , Diallo Mawlouth 
Published: July 2020

Abstract
Background
Only the sylvatic and zoophilic population of Aedes aegypti was formerly identified in southeastern Senegal. A newly established anthropophilic population was detected in the urban area of the Kedougou city. Because of its new behavior, this species could play a primary role in the transmission of dengue and other arboviruses in this area. Because these arboviruses have no vaccine and specific treatments, vector control remains the only effective way to control their outbreaks. Effective vector control strategies require to understand some aspects of the bioecology of the vector, specially resting behavior. The aims of this study were to investigate the sites and resting behavior of Ae. aegypti in southeastern Senegal.

Methods
Mosquitoes were collected in several potential resting places (rooms, tires, bricks and scrap metal) by two technicians using a CDC back-pack aspirator in the Kedougou bus station and other sites within the city and the nearby rural area. Collected mosquitoes were identified and classified.

Results
A total of 1291 mosquitoes belonging to 6 genera and 20 species were collected. Aedes aegypti was the dominant species in all the resting places investigated. This species was found resting equally in rooms, bricks, tires and scrap metal. The average number of Ae. aegypti collected in resting places was higher in the bus station (center of the city) compared to the other areas. The rates of unfed and fed females varied significantly in the different resting places while the proportions of gravid females which varied between 7.8% in tires and 1.8% in rooms were comparable.

Conclusions
This study showed that Ae. aegypti could be found resting indoors and in several sites, including in used tires outdoors. These data will be helpful in setting better arboviruses surveillance and vector control strategies.
Existence of ground state for fractional Kirchhoff equation with $L^{2}$ critical exponents
Authors: Han Yaling , Zhang Yimin 
Published: July 2020

AbstractIn this paper, we consider a class of fractional Kirchhoff equations with $L^{2}$

L
2

critical exponents. By using the scaling technique and concentration-compactness principle we obtain the existence and nonexistence of ground state for fractional Kirchhoff equation with $L^{2}$

L
2

critical exponent.
Nonlinear regularized long-wave models with a new integral transformation applied to the fractional derivative with power and Mittag-Leffler kernel
Authors: Yavuz Mehmet , Abdeljawad Thabet 
Published: July 2020

AbstractThis paper presents a fundamental solution method for nonlinear fractional regularized long-wave (RLW) models. Since analytical methods cannot be applied easily to solve such models, numerical or semianalytical methods have been extensively considered in the literature. In this paper, we suggest a solution method that is coupled with a kind of integral transformation, namely Elzaki transform (ET), and apply it to two different nonlinear regularized long wave equations. They play an important role to describe the propagation of unilateral weakly nonlinear and weakly distributer liquid waves. Therefore, these equations have been noticed by scientists who study waves their movements. Particularly, they have been used to model a large class of physical and engineering phenomena. In this context, this paper takes into consideration an up-to-date method and fractional operators, and aims to obtain satisfactory approximate solutions to nonlinear problems. We present this achievement, firstly, by defining the Elzaki transforms of Atangana–Baleanu fractional derivative (ABFD) and Caputo fractional derivative (CFD) and then applying them to the RLW equations. Finally, numerical outcomes giving us better approximations after only a few iterations can be easily obtained.
Body fat percentage assessment by skinfold equation, bioimpedance and densitometry in older adults
Authors: Silveira Erika Aparecida , Barbosa Larissa Silva , Rodrigues Ana Paula Santos , Noll Matias , De Oliveira Cesar 
Published: July 2020

Abstract
Background
Body fat estimation allows measuring changes over time attributed to interventions and treatments in different settings such as hospitals, clinical practice, nursing homes and research. However, only few studies have compared different body fat estimation methods in older adults with inconsistent results. We estimated body fat percentage (%BF) and the level of agreement among dual energy X-ray absorptiometry (DXA), bioelectrical impedance (BIA) and Durnin & Womersley’s skinfold eq. (SF) in older Brazilian adults aged 60 years and older from the Elderly Project Goiânia, Brazil.

Methods
The analytical sample comprised of 132 participants who had DXA data. The level of agreement for the %BF estimated by BIA, SF and DXA i.e. reference method, was examined using Bland and Altman’s and Lin’s plot.

Results
Overall, women had higher body mass index and %BF values measured by all three methods used. BIA and SF equation showed strong concordance to estimate body fat percentage in all participants (CCC = 0.857 and 0.861, respectively) and among women (CCC = 0.788 and 0.726, respectively) when compared to DXA. However, both methods underestimated body fat percentage in women and men with high body fat percentage. A strong level of agreement was observed between DXA and the anthropometric equation developed by Durnin & Womersley in men (CCC = 0.846), while BIA had a moderate concordance (CCC = 0.505) in this group.

Conclusion
The examined methods indicated different body fat estimates. However, the best agreement was observed between DXA and the anthropometric SF equation for men. Future research in older adults should develop new SF equations considering different ethnic groups.
Dibenzocyclooctadiene lignans from the fruits of Schisandra chinensis and their cytotoxicity on human cancer cell lines
Authors: Choi Sung-Kyu , Lee Yeong-Geun , Wang Rong Bo , Kim Hyoung-Geun , Yoon Dahye , Lee Dae Young , Kim Yeon-Ju , Baek Nam-In 
Published: July 2020

AbstractRepeated chromatographic separations of the EtOAc fraction of Schisandra chinensis fruits on silica gel, octadecyl silica gel, and Sephadex LH-20 led to the isolation and identification of seven dibenzocyclooctadiene lignans (1–7). The NMR data reported in the literature for angeloyl gomisin H (5) were shown to be incorrect. We unambiguously identified the compounds based on detailed analysis of the 1D and 2D NMR data, especially from HMBC and NOESY experiments. In addition, MTT assays and cell viability experiments verified the cytotoxicity of the isolated dibenzocyclooctadiene lignans against the human cancer cell lines AGS, HeLa, and HT-29.
A study comparing the experiences of family and friends of young people with borderline personality disorder features with family and friends of young people with other serious illnesses and general population adults
Authors: Seigerman Mirra R. , Betts Jennifer K. , Hulbert Carol , McKechnie Ben , Rayner Victoria K. , Jovev Martina , Cotton Sue M. , McCutcheon Louise , McNab Catharine , Burke Emma , Chanen Andrew M. 
Published: July 2020

AbstractBackgroundFamily and friends (‘carers’) of adults with borderline personality disorder (BPD) and carers of young people with other serious illnesses experience significant adversity but research on the experiences of caring for a young person with BPD features is sparse. This study aimed to: (i) describe the experiences of carers of young people with BPD features; (ii) compare them with published data assessing carers of young people with other serious illnesses and with adults from the general population.MethodsEighty-two carers (M age = 44.74, SD = 12.86) of 54 outpatient young people (M age = 18.76, SD = 3.02) who met 3 to 9 DSM-IV BPD criteria completed self-report measures on distress, experiences of caregiving, coping, and expressed emotion. Independent-samples t-tests were employed to compare scores with those reported by convenience comparison groups of general population adults or carers of young people with eating disorders, cancer, or psychosis.ResultsCarers of young people with BPD features reported significantly elevated levels of distress, negative caregiving experiences, and expressed emotion, as well as maladaptive coping strategies, compared with general population adults or carers of young people with other serious illnesses.ConclusionsCarers of young people with BPD features experience elevated levels of adversity compared with their peers in the general adult population. This adversity is similar to, or greater than, that reported by carers of young people with other severe illnesses. Research is needed to clarify factors underlying adverse caregiving experiences and to develop and evaluate interventions to support carers of young people with BPD features.Trial registrationProspectively registered with the Australian New Zealand Clinical Trial Registry ACTRN12616000304437 on 08 March 2016,https://anzctr.org.au/Trial/Registration/TrialReview.aspx?id=369867.
Influence of antihypertensive medications on the clinical parameters of anodized dental implants: a retrospective cohort study
Authors: Seki Keisuke , Hasuike Akira , Iwano Yoshihiro , Hagiwara Yoshiyuki 
Published: July 2020

AbstractBackgroundHypertension is a chronic medical condition in which blood pressure in the arteries is elevated. Given the large proportion of dental implant patients using antihypertensive medications, it is crucial to evaluate the effects of these drugs on the clinical parameters of osseointegrated implants. The aim of the present retrospective cohort study was to evaluate the influence of antihypertensive medications on clinical peri-implant tissue parameters.MethodsThirty-five patients received a total of 77 anodized dental implants. Based on the history of the use of antihypertensive medications, the patients were divided into two groups: the group taking antihypertensive medications (AH group) and the group of healthy patients (H group). Implants were followed up clinically and radiologically, with a focus on the peri-implant soft tissue parameters probing pocket depth, bleeding on probing, modified plaque index, and marginal peri-implant bone level stability.ResultsNone of the implants were lost, and no technical failures occurred. The mean follow-up duration was 7 years and 1 month. A significant difference was observed in the probing pocket depth 3.8 ± 1.3 mm in the AH group and 3.0 ± 0.7 mm in the H group. In the AH and H groups, 26.5% (9/34) and 4.7% (2/43) of the patients were diagnosed with peri-implantitis at the implant level, respectively.ConclusionsOur findings suggest some correlations between antihypertensive medication use and clinical parameters in anodized peri-implant tissue.
Tranexamic acid and rosuvastatin in patients at risk of cardiovascular events after noncardiac surgery: a pilot of the POISE-3 randomized controlled trial
Authors: Marcucci Maura , Duceppe Emmanuelle , Le Manach Yannick , Kearon Clive , Eikelboom John W. , Pohl Kayla , Vincent Jessica , Darvish-Kazem Saeed , Srinathan Sadeesh K. , Neary John D. D. , Parlow Joel L. , Kurz Andrea , Gross Peter L. , Mrkobrada Marko , Balasubramanian Kumar , Sessler Daniel I. , Devereaux P. J. 
Published: July 2020

Abstract
Background
Surgical bleeding is associated with postoperative cardiovascular complications. The efficacy and safety of tranexamic acid (TXA) in noncardiac surgery are still uncertain. Statins may prevent perioperative cardiovascular complications. We conducted a pilot to assess the feasibility of a perioperative trial of TXA and rosuvastatin.

Methods
Using a factorial design, we randomized patients at cardiovascular risk undergoing noncardiac surgery to intravenous TXA (1 g at the start and end of surgery) or placebo, and oral rosuvastatin (40 mg before and 20 mg daily for 30 days after surgery) or placebo. Feasibility outcomes included recruitment rates, follow-up, and compliance to interventions. Clinical outcomes were secondarily explored.

Results
After 3 months, we changed the design to a partial factorial due to the difficult recruitment of statin-naive patients. Over 6 months, 100 patients were randomized in the TXA trial (49 TXA, 51 placebo), 34 in the rosuvastatin trial (18 rosuvastatin, 16 placebo). Ninety-two percent (95% CI 80–98) of TXA and 86% (95% CI 74–94) of TXA-placebo patients received the 2 study doses. Thirty-three percent (95% CI 13–59) of rosuvastatin patients and 37% (95% CI 15–65) of rosuvastatin-placebo patients discontinued the study drug. A major cardiovascular complication occurred at 30 days in 1 TXA and 6 TXA-placebo patients, and 1 rosuvastatin and no rosuvastatin-placebo patients.

Conclusions
Our pilot study supports the feasibility of a perioperative TXA trial in noncardiac surgery. Feasibility of a perioperative rosuvastatin trial is uncertain because of a high prevalence of statin use in the target population and concerns about compliance.

Trial registration
ClinicalTrials.govNCT02546648.
Gut Microbiota in T1DM-Onset Pediatric Patients: Machine-Learning Algorithms to Classify Microorganisms as Disease Linked
Authors: Biassoni Roberto , Di Marco Eddi , Squillario Margherita , Barla Annalisa , Piccolo Gianluca , Ugolotti Elisabetta , Gatti Cinzia , Minuto Nicola , Patti Giuseppa , Maghnie Mohamad , d’Annunzio Giuseppe 
Published: July 2020

Abstract

Aims
The purpose of this work is to find the gut microbial fingerprinting of pediatric patients with type 1 diabetes.

Methods
The microbiome of 31 children with type 1 diabetes at onset and of 25 healthy children was determined using multiple polymorphic regions of the 16S ribosomal RNA. We performed machine-learning analyses and metagenome functional analysis to identify significant taxa and their metabolic pathways content.

Results
Compared with healthy controls, patients showed a significantly higher relative abundance of the following most important taxa: Bacteroides stercoris, Bacteroides fragilis, Bacteroides intestinalis, Bifidobacterium bifidum, Gammaproteobacteria and its descendants, Holdemania, and Synergistetes and its descendants. On the contrary, the relative abundance of Bacteroides vulgatus, Deltaproteobacteria and its descendants, Parasutterella and the Lactobacillus, Turicibacter genera were significantly lower in patients with respect to healthy controls. The predicted metabolic pathway more associated with type 1 diabetes patients concerns “carbon metabolism,” sugar and iron metabolisms in particular. Among the clinical variables considered, standardized body mass index, anti-insulin autoantibodies, glycemia, hemoglobin A1c, Tanner stage, and age at onset emerged as most significant positively or negatively correlated with specific clusters of taxa.

Conclusions
The relative abundance and supervised analyses confirmed the importance of B stercoris in type 1 diabetes patients at onset and showed a relevant role of Synergistetes and its descendants in patients with respect to healthy controls. In general the robustness and coherence of the showed results underline the relevance of studying the microbioma using multiple polymorphic regions, different types of analysis, and different approaches within each analysis.
TMEM147 interacts with lamin B receptor, regulates its localization and levels, and affects cholesterol homeostasis
Authors: Christodoulou Andri , Maimaris Giannis , Makrigiorgi Andri , Charidemou Evelina , Lüchtenborg Christian , Ververis Antonis , Georgiou Renos , Lederer Carsten W. , Haffner Christof , Brügger Britta , Santama Niovi 
Published: July 2020

ABSTRACT
The structurally and functionally complex endoplasmic reticulum (ER) hosts critical processes including lipid synthesis. Here, we focus on the functional characterization of transmembrane protein TMEM147, and report that it localizes at the ER and nuclear envelope in HeLa cells. Silencing of TMEM147 drastically reduces the level of lamin B receptor (LBR) at the inner nuclear membrane and results in mistargeting of LBR to the ER. LBR possesses a modular structure and corresponding bifunctionality, acting in heterochromatin organization via its N-terminus and in cholesterol biosynthesis via its sterol-reductase C-terminal domain. We show that TMEM147 physically interacts with LBR, and that the C-terminus of LBR is essential for their functional interaction. We find that TMEM147 also physically interacts with the key sterol reductase DHCR7, which is involved in cholesterol biosynthesis. Similar to what was seen for LBR, TMEM147 downregulation results in a sharp decline of DHCR protein levels and co-ordinate transcriptional decreases of LBR and DHCR7 expression. Consistent with this, lipidomic analysis upon TMEM147 silencing identified changes in cellular cholesterol levels, cholesteryl ester levels and profile, and in cellular cholesterol uptake, raising the possibility that TMEM147 is an important new regulator of cholesterol homeostasis in cells.
This article has an associated First Person interview with the first author of the paper.