bluuebunny/crossref_metadata_2025_split huggingface dataset:
The additional role of virtual to traditional dissection in teaching anatomy: a randomised controlled trial
Authors: Boscolo-Berto Rafael , Tortorella Cinzia , Porzionato Andrea , Stecco Carla , Picardi Edgardo Enrico Edoardo , Macchi Veronica , De Caro Raffaele 
Published: September 2020

Abstract
Introduction
Anatomy has traditionally been taught via dissection and didactic lectures. The rising prevalence of informatics plays an increasingly important role in medical education. It is hypothesized that virtual dissection can express added value to the traditional one.

Methods
Second-year medical students were randomised to study anatomical structures by virtual dissection (intervention) or textbooks (controls), according to the CONSORT guidelines. Subsequently, they applied to the corresponding gross dissection, with a final test on their anatomical knowledge. Univariate analysis and multivariable binary logistic regression were performed.

Results
The rate of completed tests was 76.7%. Better overall test performance was detected for the group that applied to the virtual dissection (OR 3.75 with 95% CI 0.91–15.49; p = 0.06). A comparable performance between groups in basic anatomical knowledge (p 0.45 to 0.92) but not muscles and 2D-3D reporting of anatomical structures was found, for which the virtual dissection was of tendential benefit (p 0.08 to 0.13). Medical students who applied to the virtual dissection were over three times more likely to report a positive outcome at the post-dissection test than those who applied to textbooks of topographical anatomy. This would be of benefit with particular reference to the understanding of 2D–3D spatial relationships between anatomical structures.

Conclusion
The combination of virtual to traditional gross dissection resulted in a significant improvement of second-year medical students’ learning outcomes. It could be of help in maximizing the impact of practical dissection, overcoming the contraction of economic resources, and the shortage of available bodies.
Variability in the global energy budget and transports 1985–2017
Authors: Liu Chunlei , Allan Richard P. , Mayer Michael , Hyder Patrick , Desbruyères Damien , Cheng Lijing , Xu Jianjun , Xu Feng , Zhang Yu 
Published: September 2020

AbstractThe study of energy flows in the Earth system is essential for understanding current climate change. To understand how energy is accumulating and being distributed within the climate system, an updated reconstruction of energy fluxes at the top of atmosphere, surface and within the atmosphere derived from observations is presented. New satellite and ocean data are combined with an improved methodology to quantify recent variability in meridional and ocean to land heat transports since 1985. A global top of atmosphere net imbalance is found to increase from 0.10 ± 0.61 W m−2 over 1985–1999 to 0.62 ± 0.1 W m−2 over 2000–2016, and the uncertainty of ± 0.61 W m−2 is related to the Argo ocean heat content changes (± 0.1 W m−2) and an additional uncertainty applying prior to 2000 relating to homogeneity adjustments. The net top of atmosphere radiative flux imbalance is dominated by the southern hemisphere (0.36 ± 0.04 PW, about 1.41 ± 0.16 W m−2) with an even larger surface net flux into the southern hemisphere ocean (0.79 ± 0.16 PW, about 3.1 ± 0.6 W m−2) over 2006–2013. In the northern hemisphere the surface net flux is of opposite sign and directed from the ocean toward the atmosphere (0.44 ± 0.16 PW, about 1.7 ± 0.6 W m−2). The sea ice melting and freezing are accounted for in the estimation of surface heat flux into the ocean. The northward oceanic heat transports are inferred from the derived surface fluxes and estimates of ocean heat accumulation. The derived cross-equatorial oceanic heat transport of 0.50 PW is higher than most previous studies, and the derived mean meridional transport of 1.23 PW at 26° N is very close to 1.22 PW from RAPID observation. The surface flux contribution dominates the magnitude of the oceanic transport, but the integrated ocean heat storage controls the interannual variability. Poleward heat transport by the atmosphere at 30° N is found to increase after 2000 (0.17 PW decade−1). The multiannual mean (2006–2013) transport of energy by the atmosphere from ocean to land is estimated as 2.65 PW, and is closely related to the ENSO variability.
Characterizing dynamics of serum creatinine and creatinine clearance in extremely low birth weight neonates during the first 6 weeks of life
Authors: van Donge Tamara , Allegaert Karel , Gotta Verena , Smits Anne , Levtchenko Elena , Mekahli Djalila , van den Anker John , Pfister Marc 
Published: September 2020

AbstractBackgroundCharacterizing the dynamics of serum creatinine concentrations (Scr) and associated creatinine clearance (CLcr) as a measure of kidney function in extremely low birth weight (≤ 1000 g; ELBW) neonates remains challenging.MethodsWe performed a retrospective study that included longitudinal Scr (enzymatic assay) data from 148 ELBW neonates up to 6 weeks after birth. Change of Scr and inter-individual variability was characterized with nonlinear mixed-effect modeling. Key covariates such as gestational age (GA), mode of delivery (MOD), and treatment with ibuprofen or inotropic agents were investigated.ResultsA total of 2814 Scr concentrations were analyzed. GA was associated with Scr at birth (higher with advancing GA), and GA and MOD showed an association with postnatal maturation of CLcr (faster clearance increase with advancing GA and after C-section). Small CLcr decrease (≤ 5%) was quantified during ibuprofen treatment. For a GA of 27 weeks, mean Scr (estimated CLcr) at birth was 0.61 mg/dl (0.23 ml/min), increasing to 0.87 mg/dl (0.27 ml/min) at day three, and decreasing to 0.36 mg/dl (0.67 ml/min) at day 42 after birth.ConclusionsWe report the first mathematical model able to characterize Scr and CLcr in ELBW neonates during the first 6 weeks of life in a quantitative manner as a function of GA, MOD, and ibuprofen treatment. This model allows the derivation of GA-adjusted reference ranges for ELBW neonates and provides a rationale for normative Scr concentrations, and as such will help clinicians to further optimize monitoring and treatment decisions in this vulnerable patient population.
Evaluation eines frühen interdisziplinären multimodalen Assessments für Patienten mit Schmerzen
Authors: Kaiser Ulrike , Petzke Frank , Nagel Bernd , Marschall Ursula , Casser Hans-Raimund , Isenberg Thomas , Kohlmann Thomas , Lindena Gabriele ,   PAIN2020, Augustin Katharina , Althoff Carolin , Heissenberger Josef , Hölscher Andreas , Szczotkowski Daniel , Schulz Katja , Metz-Oster Beatrice , Rensland Jana , Milch Lena , Pfingsten Michael , Schouten Leonie , Deppe Karin , Gärtner Anne , Hoffmann Greta , Preissler Anke , Pritzke-Michael Julia 
Published: September 2020

ZusammenfassungDie Versorgung von Patienten mit Schmerzen und Chronifizierungsrisiko ist nach wie vor gekennzeichnet durch Über‑, Fehl- und Unterversorgung. Das Projekt PAIN2020 (Innovationsfonds 01NVF17049) hat zum Ziel, durch die Einführung eines frühzeitigen, auf Schmerz spezialisierten interdisziplinären diagnostischen Ansatzes die ambulante Versorgung von Patienten im Hinblick auf Schmerzen und das Funktionsniveau zu verbessern. Im Rahmen einer randomisierten kontrollierten Studie werden bundesweit in 31 Einrichtungen der Regelversorgung (schmerzspezialisiertes Angebot) Patienten mit Risikofaktoren bei bestehenden Schmerzen einer frühen Schmerzdiagnostik zugeführt. Die Interventionsbedingung besteht dabei in einem interdisziplinären multimodalen Assessment mit den beteiligten Disziplinen Schmerzmedizin, Physiotherapie und Psychologie. Die Kontrollbedingung umfasst einen einmaligen Termin bei einem Schmerztherapeuten der Qualitätssicherungsvereinbarung Schmerzmedizin bzw. mit Zusatzbezeichnung spezielle Schmerztherapie. Patienten und Vorbehandler erhalten entsprechend der Befunde detaillierte Empfehlungen für eine weitere bedarfsgerechte Behandlung. Es sind 2 Evaluationsansätze geplant. Für den ersten beträgt die zu erreichende Nettofallzahl 3840 Patienten, deren klinische Daten (Deutscher Schmerzfragebogen, zusätzliche Skalen) längsschnittlich erhoben (Einschluss, 3 und 6 Monate nach Diagnostik) und auf Grundlage eines Mehr-Ebenen-Modells ausgewertet werden. Im Rahmen eines 2. Ansatzes werden diese klinischen Daten einerseits um Sekundärdaten der BARMER ergänzt sowie die Patienten des ersten Evaluationsansatzes mit BARMER-Versicherten gematcht, die an dem Projekt nicht teilgenommen haben. Die Auswertung übernimmt ein externes Evaluationsinstitut. Das Projekt startete im April 2018.
Musical practice and BDNF plasma levels as a potential marker of synaptic plasticity: an instrument of rehabilitative processes
Authors: Minutillo Alessandro , Panza Gabriele , Mauri Massimo Carlo 
Published: September 2020

Abstract
Background and objectives
The aim of the study was to investigate the influence of musical practice on brain plasticity. BDNF (brain-derived neurotrophic factor) is a neurotrophin involved in neuroplasticity and synaptic function.

Materials and methods
We recruited 48 healthy subjects of equal age and sex (21 musicians and 27 non-musicians). All subjects were administered the AQ (Autism-Spectrum Questionnaire) and plasma levels (PLs) of BDNF, oxytocin (OT), and vasopressin (VP) were measured in the blood sample of every participant.

Results.
The difference between BDNF PLs in the two groups was found to be statistically significant (t = − 2.214, p = 0.03). Furthermore, oxytocin (OT) PLs and musical practice were found to be independent positive predictors of BDNF PLs (p < 0.04). We also found a negative correlation between BDNF PLs and AD (attention to detail) sub-scale score of AQ throughout the whole sample. Assuming BDNF PLs to be a marker of synaptic plasticity, higher PLs could be associated with the activation of alternative neural pathways: a lower score in the “attention to detail” sub-scale could imply greater flexibility of higher cerebral functions among musicians.
Further researches should be conducted to assess the rehabilitative usefulness of these findings among patients affected by psychiatric disorders.
Recent outbreaks of severe hepatitis A virus infections in Vienna
Authors: Bauer David , Farthofer Anna , Chromy David , Simbrunner Benedikt , Steininger Lisa , Schmidbauer Caroline , Binter Teresa , Trauner Michael , Mandorfer Mattias , Schmidt Ralf , Mayer Florian , Holzmann Heidemarie , Strassl Robert , Reiberger Thomas 
Published: September 2020

AbstractTo explore the epidemiology and clinical course of hepatitis A virus (HAV) infections at the Vienna General Hospital. We retrospectively identified patients who were tested positive for HAV-IgM at the Vienna General Hospital form Q1/2008 to Q3/2018. Our definition of severe HAV infection was AST and/or ALT > 5 × above the upper limit of normal (ULN); and liver dysfunction as (i) hepatic encephalopathy or ammonia > 100 μmol/L, (ii) coagulopathy with INR > 1.5, or (iii) jaundice with bilirubin > 5 mg/dL. A total of 578 HAV-IgM (+) were identified, including 31 (5.4%) and 38 (6.6%) without and with liver dysfunction, respectively. A proportional increase in severe HAV cases with and without liver dysfunction occurred in 2016/2017 with (21.5% (vs. 8.0% in the years before; p < 0.001). Thirty-seven (53.6%) patients with severe HAV were hospitalized, 6 (9%) required ICU support, and one patient received liver transplantation within 30 days. Patients with severe HAV and liver dysfunction were more often male (60.5 vs. 43.1%, p = 0.055) and younger (31.5 vs. 63 years, p < 0.001) as compared with other HAV-IgM (+) cases. The observed increase of severe HAV infections in Vienna in 2017 among young males, coincided with a multinational HAV outbreak among MSM. Our data suggests a higher likelihood of severe courses of hepatitis A in MSM. Vaccination against HAV should be recommended for risk groups.
What protects older Romanians in Switzerland from loneliness? A life-course perspective
Authors: Ciobanu Ruxandra Oana , Fokkema Tineke 
Published: September 2020

AbstractThe topic of loneliness among older migrants has recently gained scholarly interest. There is a particular focus on why older migrants are generally lonelier than their non-migrant peers from the destination. These studies neglect variations both within and between older migrant groups. Our qualitative study is innovative for three reasons. First, it focuses on Romanian migrants aged 65+ who fled communism and aged in place in Switzerland—an understudied population of former political refugees that experiences little or no loneliness in later years. Second, it takes a life-course approach to explore experiences of loneliness during communist Romania, in the context of migration and later in life. Third, it focuses on protective and coping factors rather than risk factors. Having been through hard times in communist Romania—marked by fear and distrust among people and estrangement from society—older Romanian migrants built strength to withstand difficult times, learned to embrace solitude, and/or to relativise current hardships, if any. Upon arrival many founded or joined an association or church, which offers the opportunity to establish a sustainable social network consisting of a large pool of Romanian non-kin with a shared past and experience of migration and integration, to counteract social losses in later life. When moments of loneliness cannot be prevented (e.g. due to death of a spouse), they try to be active to distract from loneliness or ‘simply’ accept the situation. These aspects need to be taken into account in future research and when developing loneliness interventions.
Seismic collapse assessment of new European single-story RC precast buildings with weak connections
Authors: Cimmino Maddalena , Magliulo Gennaro , Manfredi Gaetano 
Published: September 2020

AbstractCapacity design, according to the modern seismic building codes, requires the application of specific rules and construction details in order to prevent brittle failure modes at material, element and structural level. In particular, with reference to single-story precast reinforced concrete structures with columns joined by pinned beams, the Italian seismic building code, following the Eurocode 8 general principles, requires that beam-to-column connection should be designed in order to avoid the connection failure before the formation of the plastic hinge at the column base. However, no specific details are provided in order to reach such a performance. Recent European earthquakes showed that seismic performance of beam-to-column dowel connections can be very poor. Hence, for European typical dowel beam-to-column connections, this study aims to investigate: (a) possible connection configurations obtained according to seismic design; (b) possible failure modes involving connection elements (steel dowel, transversal steel reinforcement, dowel concrete cover); (c) how available formulations are able to describe such failure modes and the related shear strength; (d) influence of connection failure on the global seismic safety at collapse of new designed single-story RC precast buildings. The reference buildings and beam-to-column connections are designed considering different geometrical layouts of the primary structure and different seismic hazard levels, i.e. four sites and two soil types. Structural capacity is estimated considering global, i.e. related to column plastic hinge degradation measured in terms of top lateral displacements, and local, i.e. related to connection shear strength, failure modes. Nonlinear multi-stripe analyses are performed for the seismic assessment.
Community completeness as a measure of restoration success: multiple-study comparisons across ecosystems and ecological groups
Authors: Noreika Norbertas , Pärtel Meelis , Öckinger Erik 
Published: September 2020

AbstractWhen restoring habitat for biodiversity, the most effective outcome will be achieved by restoration projects which target several organism groups or ecosystem types. Such integrated approaches require direct comparisons among different ecological communities while evaluating success of restoration. The Community Completeness Index (CCI) is a recently developed metric that allows such comparisons by accounting for both present and absent but otherwise suitable taxa. We empirically evaluated the applicability of CCI for assessing the outcome of ecological restoration. We analyzed how species richness and the completeness of ecological communities recover after restoration, for different ecological groups and ecosystem types, and how it develops over time after restoration. Analyses were performed on 18 datasets with per site presence-absence data from Northern Europe. Each dataset represented one of the three habitat types (mire, forest, grassland) and different ecological groups (plants, flying insects, epigeic invertebrates). Datasets contained pristine, degraded and restored sites. We calculated the dark diversity and subsequently CCI based on species co-occurrences. Our multiple-study analyses revealed that CCI of grassland plant communities increased faster after restoration than invertebrate communities or plant communities in forests and mires. In addition, flying insect communities demonstrated significantly highest CCI in pristine mires. Some results were significant only for richness but not for CCI indicating species pool effect. Finally, completeness and species richness of restored communities increased with time since restoration. As such, our study demonstrated that CCI is a useful tool in evaluating restoration success across different organism groups and ecosystem types.
Prospects and limitations of expansion microscopy in chromatin ultrastructure determination
Authors: Kubalová Ivona , Schmidt Černohorská Markéta , Huranová Martina , Weisshart Klaus , Houben Andreas , Schubert Veit 
Published: September 2020

AbstractExpansion microscopy (ExM) is a method to magnify physically a specimen with preserved ultrastructure. It has the potential to explore structural features beyond the diffraction limit of light. The procedure has been successfully used for different animal species, from isolated macromolecular complexes through cells to tissue slices. Expansion of plant-derived samples is still at the beginning, and little is known, whether the chromatin ultrastructure becomes altered by physical expansion. In this study, we expanded isolated barley nuclei and compared whether ExM can provide a structural view of chromatin comparable with super-resolution microscopy. Different fixation and denaturation/digestion conditions were tested to maintain the chromatin ultrastructure. We achieved up to ~4.2-times physically expanded nuclei corresponding to a maximal resolution of ~50–60 nm when imaged by wild-field (WF) microscopy. By applying structured illumination microscopy (SIM, super-resolution) doubling the WF resolution, the chromatin structures were observed at a resolution of ~25–35 nm. WF microscopy showed a preserved nucleus shape and nucleoli. Moreover, we were able to detect chromatin domains, invisible in unexpanded nuclei. However, by applying SIM, we observed that the preservation of the chromatin ultrastructure after the expansion was not complete and that the majority of the tested conditions failed to keep the ultrastructure. Nevertheless, using expanded nuclei, we localized successfully centromere repeats by fluorescence in situ hybridization (FISH) and the centromere-specific histone H3 variant CENH3 by indirect immunolabelling. However, although these repeats and proteins were localized at the correct position within the nuclei (indicating a Rabl orientation), their ultrastructural arrangement was impaired.
A phase 1 study of crenigacestat (LY3039478), the Notch inhibitor, in Japanese patients with advanced solid tumors
Authors: Doi Toshihiko , Tajimi Masaomi , Mori Joji , Asou Hiroya , Inoue Koichi , Benhadji Karim A. , Naito Yoichi 
Published: September 2020

SummaryBackground This phase 1, single-center, nonrandomized, single-arm, open-label, dose-escalation study, evaluated the tolerability of crenigacestat, a γ-secretase inhibitor as an oral Notch inhibitor in Japanese patients with advanced solid tumors. Methods The study consisted of 2 dose levels of crenigacestat (25 mg and 50 mg), administered orally 3 times per week (TIW) over a 28-day cycle until disease progression, development of unacceptable toxicity, or any other discontinuation criteria were met. The primary objective was to evaluate the tolerability and determine the recommended dose of crenigacestat for Japanese patients. Secondary objectives were to characterize the safety and toxicity, the pharmacokinetic parameters, and to document any antitumor activity of crenigacestat. Results Eleven Japanese patients with advanced solid tumors were enrolled; 4 patients (median age of 64 years) received 25 mg of crenigacestat, and 7 patients (median age of 72 years) received 50 mg of crenigacestat. Median treatment duration was 8 weeks in the 25-mg treatment arm and 4 weeks in the 50-mg treatment arm. There were no dose-limiting toxicities or dose-limiting equivalent toxicities observed. None of the patients had a complete or partial response to the treatment. One patient (14.3%) with a desmoid tumor in the 50-mg treatment arm showed tumor size shrinkage of 22.4% and had stable disease for 22.5 months. Frequent (>14%) treatment-related-adverse events in both treatment arms included diarrhea, malaise, and vomiting. Conclusions Crenigacestat was tolerated in Japanese patients but with limited clinical activity. The recommended crenigacestat dose in Japanese patients is 50 mg TIW.Trial registration: NCT02836600 (ClinicalTrials.gov) registered on July 19, 2016.
Effect of age on the mercury sensitivity of zebrafish (Danio rerio) sperm
Authors: Pataki Bernadett , Roberta Berta Izabella , Gazsi Gyöngyi , Urbányi Béla , Kollár Tímea , Horváth Ákos 
Published: September 2020

AbstractThe effect of age on the sensitivity of zebrafish sperm against mercury exposure was investigated in the present study. Although results of the use of sperm from mature individuals for toxicity tests have been published, there is no information about the exact age of the fish in some cases, which can affect the results. During the experiments, pooled sperm was stripped from males of 7, 12, or 18 months of age, divided into 5 sub-groups, diluted with different concentrations of Hg (0, 0.5, 1, 2.5, and 5 mg/L Hg), and incubated for 240 min. The motility parameters of sperm (progressive motility (%), curvilinear velocity (VCL)) were measured by a computer-assisted sperm analysis system, at 30, 120, and 240 min of exposure. Regarding the age, significant differences were found in PMOT (p = 0.0267) as well as in VCL (p = 0.0004) among the three different age groups. The different concentrations of Hg also caused significant differences. The most significant differences in PMOT were between the 7- and 18-month-old groups; these differences were observed at 0.5, 1 and 2.5 mg/L Hg at 30 min, at 0.5 and 1 mg/L at 120 min, as well as at 0.5 mg/L at 240 min. In VCL the most significant differences were found between the 7- and 12-month-old groups; significant differences were found at each tested concentration at 30 min as well as at 0.5 and 2.5 mg/L at 240 min. According to the results, the age of zebrafish negatively influences the sensitivity of its sperm. This may concern not only toxicology tests but many techniques in fish breeding where the sperm is treated before use (cryopreservation, pressure shock, etc.).