Abstract 1000: High multiplex immune profiling of the bone marrow tumor microenvironment identifies prognostic biomarkers in hairy cell leukemia
Authors: Koldej Rachel , Prabahran Ashvind , Ritchie David 
Published: September 2020

Abstract
Hairy Cell Leukemia (HCL) is a rare CD20+ B cell malignancy characterized by rare circulating “hairy” B cells, extensive bone marrow (BM) infiltration, splenomegaly and pancytopenia. Standard frontline treatment is the purine analogue Cladrabine (CDA) for which the duration of response is highly variable and 50% of patients relapse within 10 years. We hypothesized that analysis of the BM tumor microenvironment would identify prognostic biomarkers of response to CDA.
To date analysis of HCL immunology has been complicated by the rarity of patient samples. We were the first in the world to apply NanoString GeoMX™ digital spatial profiling (DSP) to the analysis of the expression of proteins in BM trephine samples, opening up archival samples (with accompanying long term patient follow up) in pathology departments to translational research.
In this study, we assessed HCL BM immunology pre and post CDA treatment to provide an in depth analysis of HCL immunobiology and identify treatment response biomarkers. Matched diagnostic and post CDA BM trephines from 9 HCL patients (50.2 ± 11.2 years) and 10 age matched controls (50.6 ± 13.4 years) were analyzed using DSP for the expression of 57 proteins using an immunology focused panel. 6 x 300μm regions identified by dual CD3/CD45 immunofluorescent staining were analyzed per BM trephine. As the cellularity of the BM varied across samples, the results were normalized to the nuclei count.
The expression of the immune modulators CTLA-4, STING and VISTA was significantly downregulated at HCL diagnosis compared with control BM and did not recover post CDA suggesting ongoing immune dysregulation regardless of response to treatment. BCL-2, HLA-DR and CD45 were significantly upregulated at diagnosis and returned to control levels post treatment.
To identify prognostic biomarkers, patients were classified according to their subsequent clinical course into durable vs non-durable responders. Durable responders exhibited long term responses to their initial CDA treatment (9.5 ± 4.1 years) while non-durable responders exhibited short term responses (2.25 ± 1.3 years) and required re-treatment. Durable responders had significantly increased expression of B7-H3 and decreased CD34 and FoxP3 at diagnosis and post CDA treatment exhibited significantly increased CD8, GZMB and CD68 expression suggestive of an increased environment of antigen presentation and significantly decreased CD20 expression suggestive of a deeper depletion of CD20-expressing tumor cells.
This is the first study to provide a high multiplex analysis of HCL BM microenvironment demonstrating significant immune dysregulation of the BM that does not recover with therapy and identify biomarkers of response to standard of care CDA. Prospective application of these biomarkers will allow early identification and increased monitoring/treatment in patients at increased relapse risk post CDA treatment.
Citation Format: Rachel Koldej, Ashvind Prabahran, David Ritchie. High multiplex immune profiling of the bone marrow tumor microenvironment identifies prognostic biomarkers in hairy cell leukemia [abstract]. In: Proceedings of the Annual Meeting of the American Association for Cancer Research 2020; 2020 Apr 27-28 and Jun 22-24. Philadelphia (PA): AACR; Cancer Res 2020;80(16 Suppl):Abstract nr 1000.