bluuebunny/crossref_metadata_2025_split huggingface dataset:
ANEKA PRODUK OLAHAN BUAH NENAS SEBAGAI ALTERNATIF PENINGKATAN NILAI TAMBAH PRODUK DI DESA GALANG, KABUPATEN MEMPAWAH
Authors: Indrawati Urai Suci Yulies Vitri , Hazriani Rini 
Published: October 2020

The application of PKM aims to empower organic pineapple farmers (Galang UKM) in an effort to innovate pineapple-based product diversification, so that people can get to know a variety of pineapple products (pineapple syrup) and know the benefits of consuming pineapple syrup in which marketing is still quite extensive, especially for the region outside Mempawah Regency. In addition, participants were given attractive labeling and packaging design technology, good and correct bookkeeping training in recording the benefits of marketing this syrup. In its activities, the lecturer team was assisted by 3 students. Activities in this service include the manufacture of hygienic pineapple syrup. Designing more hygienic and attractive packaging typical of Galang UKM, Financial bookkeeping training for UKM and at the end of the activity, an evaluation of the programs carried out by the lecturer team, which aims to find out the obstacles / difficulties encountered during the activity process, and then discussed to be completed followed by an exhibition of finished product businesses. It is expected that after this activity is completed, UKM Galang is able to produce pineapple processed products (pineapple syrup) that have good quality and help the Food Security Program in West Kalimantan Province.

Keywords: Various processed, label design, pineapple, empowerment, pineapple syrup.

ABSTRAK
Penerapan PKM ini bertujuan untuk pemberdayaan masyarakat tani nenas organik (UKM Galang) dalam upaya melakukan inovasi diversifikasi produk berbasis nenas , sehingga masyarakat dapat mengenal beraneka produk nenas (sirup nenas) dan mengetahui manfaat apabila mengkonsumsi sirup nenas yang mana pemasarannya masih cukup luas terutama untuk wilayah di luar Kabupaten Mempawah. Selain itu kepada peserta di berikan teknologi mendesign label dan kemasan yang menarik, pelatihan pembukuan yang baik dan benar dalam mencatat keuntungan dari pemasaran sirup ini. Dalam kegiatannya, tim dosen dibantu oleh 3 orang mahasiswa. Kegiatan dalam pengabdian ini yaitu pembuatan sirup nenas yang hygienis,. Mendesign kemasan yang lebih hygienis dan menarik khas UKM Galang, Pelatihan pembukuan finansial kepada UKM dan pada akhir kegiatan, dilakukan evaluasi terhadap program-program yang telah dilakukan oleh tim dosen, yang bertujuan untuk mengetahui hambatan/kesulitan yang dihadapi selama proses kegiatan, dan kemudian didiskusikan untuk diselesaikan dilanjutkan dengan pameran usaha produk yang telah jadi. Diharapkan setelah kegiatan ini selesai, UKM Galang mampu menghasilkan produk olahan nenas (sirup nenas) yang mempunyai kualitas yang baik dan membantu Program Ketahanan Pangan di Provinsi Kalimantan Barat.

Kata kunci: Aneka olahan, disain label, nenas, pemberdayaan, sirup nenas.
Knowledge, awareness and practices regarding malaria in Western Area Rural District, Sierra Leone: a household-based cross-sectional survey at the midpoint of the National Malaria Strategic Plan (2016-2020)
Authors: WANG Lili , Yin Jianhai , Zheng Canjun , Smith Samuel Juana , Ngegba Esther , Huang Xiaoxia , Kamara Anitta , Chen Xia , Wang Xu , Luo Wei , Kan Biao 
Published: October 2020

Abstract
Background Sierra Leone is a highly endemic area for malaria, and the implementation of the National Malaria Strategic Plan (2016-2020) has reached its midpoint. To provide more specific guidance for interventions in the future, a household-based cross-sectional survey was conducted to elucidate the knowledge, attitudes and practices regarding malaria and malarial control measures among the general public.Methods Three communities (Grafton, Jui and Kossoh) in the Western Area Rural District that were in close proximity to Sierra Leone’s capital city of Freetown were included. Households were randomly selected and interviewed with a structured questionnaire covering malaria infection, diagnosis, treatment and prevention, as well as knowledge of malaria prevention, in collaboration with the National Malaria Control Program of the Ministry of Health and Sanitation, Sierra Leone. Results A total of 262 qualified questionnaires were included. The average cost for meals per day is approximately 30000 Leones in each household. The rate of awareness, indicated by reporting having heard of malaria, was 98.1% (257/262), and 86.6% (227/262) of the respondents knew that mosquito bites are the main route of transmission. In addition, 80.9% (212/262) of the respondents sought health advice or treatment for the illness, and a similar percentage of respondents had been tested for malaria, mostly with rapid diagnostic tests (RDTs). A hight demand for long-lasting insecticidal nets (72.1%) matched the serious shortage (61.8%, 162/262), and of the households that reported a lack of nets, 66 had children younger than five years old.Conclusion The malaria burden is still high. Although public awareness of malaria prevention is high, based on this survey, there is a shortage of the necessary supplies and a limited use of preventive measures in these three communities. Therefore, the public’s knowledge of malaria should be sustained and reinforced, and the distribution and use of malaria prevention measures should be promoted to supprt the achievement of the planned objectives.
A Cassava Protoplast System for Screening Genes Associated with the Response to South African Cassava Mosaic Virus.
Authors: Chatukuta Patience , Rey Marie Emma Christine 
Published: October 2020

Abstract
Background: The study of transient gene expression in cassava plants during virus infection using existing protocols is laborious and may take approximately fifteen weeks due to cassava’s recalcitrance to transformation. The combination of a protoplast system with CRISPR-mediated gene editing promises to shorten the turnaround time from plant tissue culture to high-throughput gene expression screening for candidate genes. Here, we detail a protocol for screening genes associated with the response to South African cassava mosaic virus(SACMV) in cassava protoplasts, with reference to the ubiquitin E3 ligase gene, MeE3L.Methods: Cassava protoplasts of model, and SACMV-susceptible and -tolerant genotypes, were transformed with SACMV infectious clones and/or a CRISPR-editing construct targeting the MeE3L using PEG4000-mediated transfection. DNA and RNA were extracted from transformed protoplasts at 24 hours post-transfection. Relative SACMV DNA accumulation was determined using DpnI-digested total DNA via qPCR, MeE3L relative expression was determined via reverse transcriptase qPCR, and results were analysed using one-way ANOVA, Tukey’s HSD test and the 2-ΔΔCTstatistical method. The MeE3Lexonic region was sequenced on the ABI 3500XL Genetic Analyzer platform; and sequences were analysed for mutations using MAFTT and MEGA-X software. Construction of a phylogenetic tree was done using the Maximum Likelihood method and Jones-Taylor-Thornton (JTT) matrix-based model.Results: The differential expression of unedited and mutant MeE3L during SACMV infectionof model, susceptible and tolerant cassava protoplasts was determined within 7 weeks after commencement of tissue culture. The study also revealed thatSACMV DNA accumulation is cassava genotype-dependent and induces multiple mutations in the tolerant landrace MeE3L homolog. Notably, the susceptible cassava landrace encodes a RINGlessMeE3Lwhich is silenced by SACMV-induced mutations.SACMV also induces mutations which silence the MeE3L RING domain in protoplasts from and tolerant cassava landraces.Conclusions: This protocol presented here halves the turnaround time for high-throughput screening of genes associated with the host response to SACMV. It provides evidence that a cassava E3 ligase is associated with the response to SACMV and forms a basis for validation of these findings by in plantafunctional and interaction studies
A Matched-pair Analysis on the Effect of Migration Background on Response Rates and Survival of Cancer Patients Treated at a Comprehensive Cancer Center in Germany
Authors: Rüdiger Roman , Geiser Franziska , Ritter Manuel , Brossart Peter , Keyver-Paik Mignon-Denise , Faridi Andree , Vatter Hartmut , Bootz Friedrich , Landsberg Jennifer , Kalff Jörg C. , Herrlinger Ulrich , Kristiansen Glen , Pietsch Torsten , Aretz Stefan , Thomas Daniel , Radbruch Lukas , Kramer Franz-Josef , Strassburg Christian P. , Gonzalez-Carmona Maria , Skowasch Dirk , Essler Markus , Schmid Matthias , Nadal Jennifer , Ernstmann Nicole , Funke Benjamin , Schmidt-Wolf Ingo G.H. 
Published: October 2020

Abstract
Background: There are several studies that investigate health inequalities in Germany due to its being a destination for immigrants since the 1960s. In this study, we pay specific attention to differences in cancer survival between German patients with and without a migration background being treated at a comprehensive cancer center. This becomes of particular interest since previous studies suggested that immigrant patients have a worse response to treatment and lower survival rates in their new host country.Methods: We considered 8162 cancer patients being treated at the CIO Bonn of the University Hospital of Bonn between April 2002 and December 2015 for our matched-pair analysis. Patients with migrant background were identified using a manual name-based algorithm with high specifity and then matched with patients without migrant background adjusting demographic characteristics, tumor features, defined staging criteria and primary therapy. Finally, 211 patients with a migrant background were matched to 211 patients without a migrant background and then compared with regard to response to treatment and survival rates (i.e. Overall survival, Progression-free survival and Time to progression).Results: Compared to the cohort with migration background, the cohort without migration background is slightly older (57.9 vs. 54.9 years) with equal gender distribution (55.0% vs. 54.5% female) and had a longer follow-up time (42.6 vs. 36.9 months). We did not find significant differences in cancer survival (overall survival; P=0.802) and response rates (ORR; McNemar's test, P=0.3458) between both collectives.Conclusion: In contrast to previous studies, we found no evidence that migration background of cancer patients being significantly affects response rates and survival. A worse outcome in populations of immigrant origin most likely has complex reasons, but highly standardized procedures in a comprehensive cancer center may contribute positively to reducing detrimental effects.
The influence of haematuria on the utility of the BTA stat test in the diagnosis of bladder cancer: a prospective study
Authors: Xiong Xiong , He Wei , Xiong Bin , Yao Feng 
Published: October 2020

Abstract
Background: The urinary bladder tumour antigen (BTA) stat test has already been used for the diagnosis and monitoring of bladder cancer (BC). However, more evidence is needed regarding its efficacy and utility in the clinic. In this study, we investigated the influence of haematuria on the performance of the BTA stat test in a clinical cohort. Methods: Urine samples from 836 subjects, including 50 healthy volunteers, 553 patients with benign urologic disorders, 124 patients with histologically proven BC, and 109 patients with other histologically proven urologic cancers, were analysed by the BTA stat test and urinalysis. We detected the sensitivity and specificity of the BTA stat test in each group, and analysed the effect of haematuria on the specificity. Results: Our data showed that 58.06% of patients had haematuria in the BC group. Haematuria with benign prostatic hyperplasia (BPH), renal hamartoma (RH) and urolithiasis were identified in 39.01%, 42.86% and 66.49% of patients with benign urologic disorders, respectively. Haematuria was identified in 48.72% of prostatic cancer patients and 67.74% of renal cancer patients. The overall sensitivity of the BTA stat test was 90.32%. The sensitivity was 97.22% in BC patients with haematuria and 80.77% in BC patients without haematuria. The overall specificity in healthy individuals, patients with benign urologic disorders and other urologic cancers was 50.84%. In all patients with haematuria, the specificity of the BTA stat test was 15.82%, while it was 72.6% in patients without haematuria. Conclusions: Haematuria has a significant influence on the BTA stat test. Thus, this test should not be used for the diagnosis of bladder cancer in patients with haematuria.
Diagnostic Value of Contrast-enhanced Digital Mammography Versus Contrast-enhanced MRI for Detecting Residual Disease after Neoadjuvant Chemotherapy for Breast Cancer
Authors: Kim Eun Young , Yun Ji-Sup , Park Yong Lai , Park Chan Heun , Kwon Mi-Ri , Moon Ju Hee , Youn Inyoung , Choi Yoon Jung , Kook Shin Ho 
Published: October 2020

Abstract
Background: Preoperative evaluation of breast cancer using contrast-enhanced digital mammography (CEDM) as a possible alternative to contrast-enhanced magnetic resonance imaging (CEMRI) has gained acceptance. Our aim is to compare the diagnostic performance of CEDM and CEMRI for chemotherapeutic response in breast cancer patients who underwent neoadjuvant chemotherapy (NAC).Methods: From November 2017 to October 2018, 30 patients with invasive carcinoma who performed both CEDM and CEMRI were included. Residual malignancy sizes after NAC were compared with histopathological results. The diagnostic performances for detecting residual cancers were compared using Lin concordance and Pearson correlation coefficients.Results: Thirty patients were included for the analysis. Mean tumor size after NAC was 1.22 cm (range: 0–7.0 cm) for CEDM and 1.13 cm (range: 0–5.1cm) for CEMRI compared with 1.89 cm (range: 0–12.0 cm) at final pathology measurement. Sensitivity for identifying residual lesion was for CEDM and CEMRI is as follows (CEDM 62.5%, 95% CI 40.6–81.2, CEMRI 66.7%, 95% CI 44.7–84.4). The positive predictive value (PPV) for residual lesion was 93.8% (95% CI 69.8–99.8) for CEDM and 88.9% (95% CI 65.3–98.6) for CEMRI. CEDM had a mean difference from pathology measurement of 0.668 cm, with a concordance coefficient of 0.202 and a Pearson correlation coefficient of 0.231 (p=0.220).Conclusions: Diagnostic value of CEDM for detecting residual tumor extent after NAC was comparable to that of CEMRI.
It May Cost an Arm and a Leg; Value on Workers and Occupational Fatality Rates in the U.S.
Authors: Klos Leah Shely , Giordano Frank B. , Stoffregen Stacy A. , Azuma Miki C. , Lee Jin 
Published: October 2020

Abstract
Background

The present study aims to observe how societal indicators of workers’ values at the state-level are related to health and safety outcomes, particularly major injuries and fatalities in the U.S. Underscoring workforce flexibility and workability over workforce stability and safety might be indicative of the worth of workers which can be associated with occupational safety and health concerns.
Methods

Multiple regression analysis was adopted to examine how the state-level indicators of values on workers in terms of 1) minimum wage, using the data from 2015; 2) average of workers’ compensations for the loss of an arm, hand, leg, or foot in 2015 were prospectively associated with occupational fatality rates in 2016 and 2017. Socioeconomic contextual variables such as education level, GDP per capita, income gap, and population at the state-level were controlled for.
Results

The present study showed that state-level quantitative indicators of how workers are valued at work, namely minimum wage and workers’ compensation benefits, were significantly and negatively associated with fatality rates in the following year. Workers’ compensation benefits were significantly and negatively associated with fatality rates two years later, implying the lasting effect of this particular type of indicator of values on workers.
Conclusions

The present study illustrates the gap in how workers are valued across the U.S.. The study speaks to the importance of fostering culture where workers are adequately valued, cared about, and protected to prevent and curtail occupational fatality.
SETD5 Binding to EP300/HIF1α is Required for Glycolysis in Breast Cancer Stem-Like Cells
Authors: Yang Zhaoting , Li Huazi , Zhang Chengye , Che Nan , Feng Ying , Liu Xingzhe , Li Mengxuan , Xuan Yanhua 
Published: October 2020

Abstract
BackgroundGlycolysis plays a pivotal role in breast cancer stem-like cell reprogramming. The SET-domain containing 5 (SETD5) is a previously uncharacterized member of the histone lysine methyltransferase family. Yet, the molecular mechanisms underlying the promotion of stem-like and glycolysis activation traits of SETD5 have not been elucidated.MethodsBasing on public datasets, we explored clinicopathological and survival analysis of SETD5 on breast cancer (BC) patients. Spheroid formation, transfection experiments and measurement of glucose uptake and lactate production analyzed the regulatory function of SETD5 on glycolysis in breast cancer stem-like cells (BCSC). The impact of SETD5 on tumor growth was studied in a murine xenograft model. Immunohistochemistry, immunofluorescence, western blot, preparation of cytoplasmic and nuclear extracts and co-immunoprecipitation were used to determine the molecular mechanisms of SETD5 in cancer cell glycolysis.ResultsOur data displayed that overexpression of SETD5 in BC tissues is positively associated with progression. SETD5 overexpression is associated with poor post-progression survival in BC patients. SETD5 expression was enriched in spheroid cells. Downregulation of SETD5 significantly decreased BCSC properties and glycolysis in vitro and in vivo. Interestingly, SETD5 and glycolytic enzymes were accumulated in the central hypoxic regions of subcutaneous tumor tissues. Our mechanistic study found that SETD5 binding to EP300/hypoxia-inducible factor 1α (HIF1α) and work as an upstream effector. SETD5 knockdown reduced the expression of HIF1α, hexokinase-2, and 6-phosphofructo-2-kinase in the nucleus after treatment with cobalt chloride (CoCl2), a chemical hypoxia mimetic agent, which activates HIF1α to accumulate in the nucleus. ConclusionSETD5 is required for glycolysis in BCSCs through binding to EP300/HIF1α and could be a potential therapeutic target for BC patients.
Regional Lymph Nodes Distribution Pattern in Central Area of Right-sided Colon Cancer: In-Vivo Detection and the Update on the Clinical Exploration.
Authors: Feng Xiaochuang , Li Hongming , Lu Xinquan , Yi Xiaojiang , Wan Jin , Liao Weilin , Wang Jiahao , Ke Yisen , Chen Jialiang , Diao Dechang 
Published: October 2020

Abstract
BackgroundDistribution of regional lymph nodes (LNs) is decisive for the lymphadenectomy boundary in radical resection of a right-sided colon cancer (RCC). Currently, the data of LNs in central area remains ambiguous and scarce. Herein we aim to provide a more detailed anatomical research on LNs surrounding the superior mesenteric vessels for RCC and investigated the metastasis rate.MethodsCarbon Nanoparticles (CNs) or Indocyanine Green (ICG) were used as dye and we laparoscopically observed the stained LNs distribution pattern and analyzed the harvested LNs combined with pathology report. Lastly, 137 RCC patients who received a “superior mesenteric artery (SMA)-oriented” hemicolectomy from September 2016 to September 2020 were included to calculate the probability of LNs metastasis in our target area.Results20 patients diagnosed as RCC (mean age 55.55 years, 13 male) were included. 13 patients underwent CNs injection and 7 patients consented to the ICG, while 4 cases suffered from imaging failure. The unequal number of the regional LNs located between SMV and SMA was detected in 17 cases (85%), posterior to SMV area in 6 cases (30%), and anterior to SMA in 11 cases (55%), respectively. The presence of LNs posterior to SMV was associated with the crossing pattern of ileocolic artery (²= 5.38, p= 0.020). The probability of LNs metastasis in the above areas (target areas) was 2.19% (3/137). No dyed LNs occurred when the SMA sheath was exposed. What’s more, the number of total harvested LNs in patients with dye injection was significant more than dye-free RCC patients (22.44±13.78 vs 43.20±22.70, p<0.01). ConclusionRight-hemi colon-draining lymphatic vessels anteriorly/posteriorly traversed the SMV and arrived at the surface of SMA near the middle colonic artery (MCA) level, which highlights the potential need of CME to place the internal border anterior to SMA and the removal of mesenteric tissue in our target area on lymphatic resection.
Russia’s Covid – 19 Vaccine: Social discussion and first emotions
Authors: R Meena , V Thulasi Bai 
Published: October 2020

Abstract
Social media chats related to healthcare are the prodigious basis of analysing the emotions of the people. Now, the Covid-19 vaccine is being the prevalent hope of nearly the entire mankind in the planet. Russia’s first vaccine announcement kindled the various rays of emotions among the social media users which are shared as tweets. The tweet data is collected and analysed for the emotions and psychology of the users along with the topic of interest in their discussion. Using computational methods and algorithms such as machine learning and LDA, the social emotions are revealed and presented.
Subclavian arteries involvement in patients with giant cell arteritis: do we need a modified Halo Score?
Authors: Collada Juan Molina , Martínez-Barrio Julia , Serrano-Benavente Belén , Castrejón Isabel , Nieto-González Juan Carlos , Motta Liz Rocío Caballero , Folguera Laura Trives , Álvaro-Gracia José María 
Published: October 2020

Abstract
Objective: To assess whether adding the subclavian arteries examination into the ultrasound (US) Southend Halo Score, as proposed in the modified Halo Score, improves the diagnostic accuracy of giant cell arteritis (GCA) and its relationship with systemic inflammation.Methods: Retrospective observational study of patients referred to a GCA fast track pathway (FTP) over a 1-year period. Patients underwent US exam of temporal and large vessel (LV) (carotid, subclavian and axillary) arteries. The extent of inflammation was measured by the halo count, the Southend Halo Score and the modified Halo Score. The gold standard for GCA diagnosis was clinical confirmation after 6 months follow-up.Results: 64 patients were evaluated in the FTP, 17(26.5%) had GCA. Subclavian arteries involvement was present only in patients with GCA (29.4% versus 0%,p<0.001). Overall, the three scores showed excellent diagnostic accuracy for GCA (ROC AUC 0.906, 0.930 and 0.928, respectively) and moderate correlations with acute phase reactants (0.35-0.51, p<0.01). Only the modified Halo Score correlated with markers of inflammation in patients with LV involvement. Conclusions: The inclusion of subclavian arteries examination in the modified Halo Score does not improve the diagnostic accuracy of GCA. Nevertheless, it correlates better with markers of systemic inflammation in LV-GCA