bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effectiveness of Cytapheresis for Ulcerative Colitis in Special Situations: Delayed Onset of Optimum Efficacy in Elderly Patients
Authors: Shibuya Tomoyoshi , Nomura Osamu , Nomura Kei , Okahara Koki , Haga Keiichi , Ishikawa Dai , Sakamoto Naoto , Ogihara Tatsuo , Osada Taro , Nagahara Akihito 
Published: November 2019

Background: Cytapheresis is a non-pharmacologic treatment option in which depleting elevated/activated leucocytes is known to exacerbate and perpetuate ulcerative colitis (UC) by releasing inflammatory cytokines. Therefore, it is a relevant treatment for elderly patients who wish to avoid pharmacologicals. Methods: The efficacy of Cytapheresis for remission induction in 72 patients who received Cytapheresis for active UC at our hospital was retrospectively evaluated. Patients included 11 elderly cases, patients on steroids, biologics, calcineurin inhibitor, and 13 with extra-intestinal complications. Lichtiger’s UC clinical activity index ≤4 meant remission was assessed at the end of therapy and then 1 month later. The efficacy on extra-intestinal manifestations meant improvement of the main morbidity. Results: At the end of Cytapheresis therapy, the remission rate in the elderly was 36.4%, and 54.2% in the non-elderly patients. One-month post Cytapheresis, the remission rate in the elderly had increased to 72.7% (p = 0.042), but to 58.3% in the non-elderly, suggesting a delayed response phenomenon in the elderly. The efficacy of Cytapheresis in 4 cases with loss of response to biologics was 75%, and 84.6% in the 13 patients with extra-intestinal complications, indicating a dramatic efficacy on dermatitis and arthralgia. Conclusions: Unlike pharmacologicals, the efficacy of Cytapheresis appears to be time dependent. Accordingly, in the elderly, we observed a delayed response, indicating that elderly patients may respond beyond the end of Cytapheresis therapy. Therefore, patients who do not show efficacy at the end of Cytapheresis therapy should be followed up for delayed response. Further, Cytapheresis is favored by patients for its good safety profile.
Ektaphelenchoides shiroodensis n. sp. (Rhabditida: Ektaphelenchinae) a new member of the genus
Authors: Aliramaji Farzad , Pourjam Ebrahim , Pedram Majid 
Published: November 2019

Summary
Ektaphelenchoides shiroodensis n. sp. is described and illustrated based upon morphological, morphometric and molecular data. It was recovered from the bark samples of a dead alder tree (Alnus sp.) from countryside around Shirood city, Mazandaran province, in the north of Iran. The new species is characterised by 768-985 μm long females, its lip region separated from the body contour by constriction, lateral field with three barely visible lines, forming two weak bands, 26-29 μm long stylet with wide lumen without conophore and basal swellings, excretory pore always at the level with median bulb, post-vulval uterine sac (PUS) 43-76 μm long, elongate conoid posterior body region ending to a long filiform part with pointed tip, males common with dorsally convex conical tail ending to a short narrower region with pointed tip, seven precloacal + cloacal + caudal papillae and arcuate spicules with wide condylus, blunt rostrum and simple end. Based upon the similar posterior body region (‘tail’) and comparable PUS length, the new species was compared to three known species, viz., E. attenuata, E. hunti and E. pini. Comparisons with the aforementioned species and similar species under the genus Seinura are discussed. The phylogenetic affinities of the new species, based upon two partial small and large subunit ribosomal DNA (SSU and LSU rDNA D2-D3) sequences, are discussed. Furthermore, several ektaphelenchid and seinurid species previously described from Iran were recovered in the present study, sequenced for their aforementioned genomic markers, and included in the phylogenetic analyses.
Identifying Disadvantaged Groups for Cochlear Implantation: Demographics from a Large Cochlear Implant Program
Authors: Tolisano Anthony M. , Schauwecker Natalie , Baumgart Bethany , Whitson Johanna , Kutz Joe Walter , Isaacson Brandon , Hunter Jacob B. 
Published: November 2019

Objective: To identify demographic predictors of patients undergoing cochlear implantation evaluation and surgery. Methods: Consecutive adult patients between 2009 and 2018 who underwent cochlear implantation evaluation at a university cochlear implantation program were retrospectively identified to determine (1) cochlear implantation qualification rate and (2) pursuit of surgery rate with respect to age, gender, race, primary spoken language, marital status, insurance type, and distance to the cochlear implantation center. Results: A total of 823 cochlear implantation evaluations were analyzed. Overall, 76.3% of patients qualified for cochlear implantation and 61.5% of these patients pursued surgery. Age was the only independent predictor for cochlear implantation qualification, such that, for each year younger, the odds of qualifying for cochlear implantation increased by 2.5% (OR 0.98; 95% CI: 0.96-0.99). Age, race, marital status, and insurance type were each independent predictors of the decision to pursue surgery. The odds of pursuing surgery increased by 2.8% for each year younger (OR 1.03; 95% CI: 1.01-1.05). Compared to White patients, non-Whites were half as likely to pursue surgery (OR 0.47; 95% CI: 0.25-0.88). Single (OR 0.49; 95% CI: 0.26-0.94) and widowed patients (OR 0.46; 95% CI: 0.23-0.95) were about half as likely to pursue surgery as compared to married patients. Patients with military insurance were 13 times more likely to pursue surgery as compared to patients with Medicare (OR 13.0; 95% CI: 1.67-101.4). Conclusion: Younger age is an independent predictor for a higher cochlear implantation qualification rate, suggesting the possibility for delayed candidacy referral. Rate of surgical pursuit in qualified cochlear implantation candidates is lower for racial minorities, single and widowed patients, and older patients.
Balancing daylight and overheating in low-energy design using CIBSE improved weather files
Authors: Brembilla Eleonora , Hopfe Christina J , Mardaljevic John , Mylona Anastasia , Mantesi Eirini 
Published: November 2019

A new set of CIBSE weather files for building performance simulation was recently developed to address the need for better quality solar data. These are essential for most building performance simulation applications, particularly for daylighting studies and low-energy building design, which requires detailed irradiation data for passive solar design and overheating risk analysis. The reliability of weather data becomes paramount when building performance is pushed to its limits. Findings illustrate how principles of good window design can be applied to a case study building, built to the Passivhaus standard, and how its expected performance is affected by the quality of solar irradiation data. Analyses using test reference years were most affected by changes in the solar radiation model (up to 8.3% points), whereas for design summer years the maximum difference was 1.7% points. Adopting the new model caused overheating risk to be classified as more severe using test reference years than design summer years, prompting a discussion on the design summer year selection method. Irradiance data measured on-site were used as a benchmark to evaluate the new solar radiation model, which was found to significantly improve the accuracy of irradiance data within weather files and so the reliability of overheating assessments. Practical application: CIBSE weather files are widely used for compliance verification of building performance in the UK context. This paper tests how the introduction of a new solar radiation model in weather files will affect daylighting and overheating simulation results. Examples are given on how low-energy building design considerations driven by advanced simulation techniques can help reaching indoor visual and thermal comfort requirements.
Duration of sweep gas off trial for weaning from venovenous extracorporeal membrane oxygenation
Authors: Na Soo Jin , Choi Hee Jung , Chung Chi Ryang , Cho Yang Hyun , Sung Kiick , Yang Jeong Hoon , Suh Gee Young , Ahn Joong Hyun , Carriere Keumhee C. , Jeon Kyeongman 
Published: November 2019

Background: No data are available on the duration of time needed to assess the adequacy of lung function after stopping sweep gas for weaning of venovenous extracorporeal membrane oxygenation (ECMO). The objective of this study was to investigate changes in arterial blood gases (ABGs) during sweep gas off trials in patients receiving venovenous ECMO. Methods: Data on patients receiving venovenous ECMO, with a weaning trial at least once, were collected prospectively from January 2012 through December 2017. Serial changes in ABGs during sweep gas off trial and clinical outcomes after weaning from venovenous ECMO were evaluated. Results: Over the study period, 192 sweep gas off trials occurred in 93 patients: 115 (60%) failed and 77 (40%) were successful. During the trial, significant changes in blood gases were observed within 1 h in all patients. When serial ABGs were compared according to trial off results, there were no significant differences in the pH, PaCO2, and HCO3− trends across time points between successful and failed trials. However, PaO2 (70.6 versus 93.4 mmHg), SaO2 (91.9 versus 95.2%), and PaO2/FiO2 ratio (164.0 versus 233.4) were significantly lower in failed trials than successful trials within 1 h after stopping sweep gas. After 2 h of trial off, no significant change in blood gases was observed until the end of the trial. Conclusions: No change in blood gases was observed 2 h after stopping sweep gas in patients receiving venovenous ECMO. Based on our institutional experience, however, we suggest monitoring for 2 h or more after stopping sweep gas flow to assess if patients are ready for decannulation. The reviews of this paper are available via the supplemental material section.
Increased incidence of systemic serious viral infections in patients with inflammatory bowel disease associates with active disease and use of thiopurines
Authors: Wisniewski Andrew , Kirchgesner Julien , Seksik Philippe , Landman Cécilia , Bourrier Anne , Nion‐Larmurier Isabelle , Marteau Philippe , Cosnes Jacques , Sokol Harry , Beaugerie Laurent ,   the Saint‐Antoine IBD network, Arrivé Lionel , Beaugerie Laurent , Bourrier Anne , Camus Marine , Chafai Najim , Chambenois Edouard , Chaput Ulriikka , Debove Clotilde , Delattre Charlotte , Dray Xavier , Fléjou Jean‐François , Le Gall Guillaume , Hoyeau Nadia , Kirchgesner Julien , Landman Cécilia , Lefèvre Jérémie H , Marteau Philippe , Martineau Chloé , Monnier‐Cholley Laurence , Nion‐Larmurier Isabelle , Ozenne Violaine , Parc Yann , Seksik Philippe , Sokol Harry , Svrcek Magali , Tiret Emmanuel 
Published: November 2019

BackgroundThe magnitude and drivers of the risk of serious viral infections in Inflammatory Bowel diseases (IBD) are unclear.ObjectiveThe objective of this study was to assess the incidence and risk factors for systemic serious viral infections in IBD patients.MethodsUsing MICISTA, a database detailing prospective characteristics and complications of IBD, we identified patients that were followed for IBD in 2005–2014 outside the context of organ transplantation, HIV infection or chronic viral hepatitis. We estimated incidences of systemic serious viral infections, defined by the need for hospitalization or permanent organ damage. Standardized incidence ratios (SIRs) were calculated using the French hospital database. We performed a case‐control study nested in MICISTA for assessing the role of exposure to IBD drugs and IBD clinical activity in the risk of developing infection.ResultsWe identified 31 patients with serious viral infections among 2645 patients followed for 15,383 person‐years. We observed 13 cases of cytomegalovirus, 10 Epstein–Barr virus, 5 varicella zoster virus and 3 herpes simplex virus infections. No deaths occurred. The incidence rate of infections in patients with IBD was 2.02/1000 person‐years, and the SIR was 3.09 (95% confidence interval (CI), 1.98–4.20; p  = 0.0002) in the study population. By multivariate analysis, increased risk of infection was associated with exposure to thiopurines (odds ratio (OR), 3.48; 95% CI, 1.36–8.90; p  = 0.009), and clinically active IBD at onset of infection (OR, 3.35; 95% CI, 1.23–9.23; p  = 0.02).ConclusionsThe incidence of systemic serious viral infections in patients with IBD is tripled compared to general population. Clinically active IBD and exposure to thiopurines are the main drivers of the risk.
Two Waves of Mitochondrion Disruption in Apoptosis: Implications for the Design of Anti-Cancer Drugs.
Authors: Chen Min , Guerrero Alan D. , Huang Li , Shabier Zainuer , Sandoval Hector , Wang Jin 
Published: November 2019

Abstract
Apoptosis plays an important role in preventing cancer development and promoting anti-cancer therapy. During mitochondria-mediated apoptosis, the disruption of mitochondria leads to the release of cytochrome c to form apoptosome with Apaf-1 and caspase-9, resulting in caspase-9 activation. However, the precise mechanisms controlling mitochondrion disruption remain elusive. Ablation of caspase-9 or other apoptosome components causes abnormal brain development and perinatal lethality in mice, making it difficult to assess the in vivo functions of caspase-9 signaling in many cell types and the role of caspase-9 in regulating mitochondria disruption. Through conditional knockout and inducible activation of caspase-9, we show that the loss of mitochondrion membrane potential (Δψm) during apoptosis proceeds in distinct caspase-9-dependent and independent steps. The initial loss of cells with high Δψm (Δψmh) was independent of caspase-9, but required BH3-only proteins of the Bcl-2 family, such as Bim and BNIP3L. Caspase-9 deficiency arrested the B cells at a stage with intermediate levels of Δψm (Δψmint). Apoptosis stimuli induced activation of effector caspases and cleavage of anti-apoptotic myeloid cell leukemia-1 (Mcl-1) in wild type but not caspase-9−/− B cells. Specific activation of caspase-9 by chemical-induced dimerization in human leukemia H9 cells also resulted in the loss of Δψm and cleavage of Mcl-1, but silencing of downstream effector caspases inhibited Mcl-1 cleavage and sequestered the cells at the Δψmint stage. Moreover, a cleavage-resistant Mcl-1 inhibited caspase-9-dependent mitochondrion disruption and apoptosis, indicating that caspase-9-mediated mitochondrion destruction involves Mcl-1 processing. Our data suggest that BH3-only proteins initiate the first wave of mitochondrion disruption during apoptosis with Δψmh to Δψmint transition, while caspase-9 signaling orchestrated the second wave of Δψmint to Δψm− transition through the cleavage of Mcl-1. Defects in mitochondria-mediated apoptosis pathway are associated with the development and treatment failures of many hematological malignancies. Identification of stepwise mitochondrial disruption may allow specific targeting of apoptotic machineries in various therapeutic settings. Chemical-induced dimerization of caspase-9 may be particularly useful for the design of anti-cancer drugs to treat those malignancies involving Mcl-1 over-expression.
Characterization of gut microbiota in children with pulmonary tuberculosis
Authors: Li Weiran , Zhu Yu , Liao Qiong , Wang Zhiling , Wan Chaomin 
Published: November 2019

AbstractBackgroundGut microbiota plays a critical role in many important physiological processes and is linked with various pulmonary infectious diseases. The relationship between pulmonary tuberculosis (PTB) and gut microbiota has been poorly studied. The present study aimed to characterize gut microbiota in pediatric patients with PTB.MethodsA case-controlled study was executed for the characterization of gut microbiota in pediatric PTB patients. Fecal samples were collected from the PTB patients and healthy controls upon admission. In addition, a one-month follow-up assessment was performed to investigate alterations in the gut microbiota post anti-tuberculosis treatment. 16SrDNA sequencing analysis of fecal DNA was completed on the Illumina MiSeq platform.ResultsCompared with healthy controls, the gut microbiota of pediatric patients with PTB was characterized by decreased microbial diversity. PTB patients further presented an up-regulation of the pro-inflammatory bacteriaPrevotella,the opportunistic pathogenEnterococcus,as well as a reduction of beneficial bacteria includingRuminococcaceae,Bifidobacteriaceaeandprausnitzii.One-month after anti-tuberculosis therapy, the richness of gut microbiota in PTB patients was distinctly depleted.ConclusionsThe gut microbiota of pediatric patients with PTB was significantly distinct from healthy controls. Additionally, the richness of gut microbiota in PTB patients decreased after one-month anti-tuberculosis treatment. It is hypothesized that the homeostasis of gut microbiota in PTB patients may affect the pathogenies of PTB by de-regulation of the hosts’ immune status through the gut-lung axis.
Clinical application of large channel endoscopic decompression in posterior cervical spine disorders
Authors: Li Chengli , Tang Xiaojie , Chen Song , Meng Yongchun , Zhang Wei 
Published: November 2019

Abstract
Background
We investigated the clinical value of posterior percutaneous endoscopic decompression (PED) for single-segment cervical spondylotic myelopathy (CSM) and cervical spondylotic radiculopathy (CSR). Methods: Clinical data from February 2016 to March 2018 were collected for 32 patients with single-segment CSM or CSR who underwent posterior cervical percutaneous large channel endoscopic decompression and completed a regular follow-up exam at 12 months after surgery. Patient data included: age (range 30–81 years and mean of 49.5 years) and surgical information (operation time, bleeding volume, hospital stay, complications, etc.). The Japan Orthopedic Association (JOA) score and pain visual analog scale (VAS) were used to evaluate the surgical outcome for each patient. Cervical spine radiographs were used to evaluate cervical curvature (Cervical spondylotic angle (CSA), C2–7 Cobb angle) and CT and MRI were used to assess the extent of laminectomy and nerve root decompression. The JOA score, VAS score, cervical curvature were analyzed statistically, and the clinical outcome was evaluated using modified Macnab criteria at the last patient follow-up exam.

Results
The JOA and VAS scores were compared before and after surgery (1 day Pre-op; 3 days, 3 months and 12 months Post-op). The differences were statistically significant (P < 0.05). There were significant differences in cervical curvature (C2–7 Cobb angle) between the time points (1 day Pre-op; 3 days, 3 months and 12 months Post-op), but the differences were no statistically significant in CSA angle (P < 0.05) The operation time range was 45–110 min (mean 68.6 ± 23.8 min); the intraoperative blood loss range was 20–85 ml (mean28 ± 14.8 ml), and the hospital stay was 3–8 days (mean4.5 days). At the last follow-up, the clinical efficacy was evaluated using modified Macnab criteria. The results were excellent in 18 cases, good in 11 cases, and fair in 3 cases. The combined excellent and good rate was 93.75%. Postoperative CT and MRI showed that the compression of the spinal cord or nerve roots was completely relieved.

Conclusion
Endoscopic decompression of posterior cervical vertebral disorders is a safe, effective, and minimally invasive surgical procedure with rapid recovery times. This procedure warrants additional research and clinical application.
Comparison between minimally invasive plate osteosynthesis and open reduction-internal fixation for proximal humeral fractures: a meta-analysis based on 1050 individuals
Authors: Li Feilong , Liu Xuqiang , Wang Fuqiang , Gu Zhiping , Tao Qianyuan , Yao Cong , Luo Xuwen , Nie Tao 
Published: November 2019

Abstract
Background
This meta-analysis aimed to compare the clinical outcomes and complications of minimally invasive plate osteosynthesis (MIPO) and open reduction–internal fixation (ORIF) in patients with proximal humeral fractures.

Methods
We searched PubMed, EMBASE, Ovid, and the Cochrane Library to identify all relevant studies from inception to April 2019. Cochrane Collaboration’s Review Manage 5.3 was used for meta-analysis.

Results
Sixteen studies involving 1050 patients (464 patients in the MIPO group and 586 patients in the ORIF group) were finally included. According to the meta-analysis, MIPO was superior to ORIF in operation time, blood loss, postoperative pain, fracture union time, and constant score. However, MIPO was associated with more exposure to radiation and axillary nerve injury. No significant differences were found in length of hospital stays and complication except for axillary nerve injury.

Conclusion
The present evidence indicates that compared to ORIF, MIPO had advantages in functional outcomes, operation time, blood loss, postoperative pain, and fracture union time for the treatment of PHFs. However, the MIPO technique had a higher rate of axillary nerve injury and longer radiation time compared to ORIF.
Hashimoto’s thyroiditis impairs embryo implantation by compromising endometrial morphology and receptivity markers in euthyroid mice
Authors: Wu Zhangbi , Cai Yaojun , Xia Qin , Liu Tiantian , Yang Hao , Wang Fen , Wang Nan , Yu Zhen , Yin Chunying , Wang Qunan , Zhu Defa 
Published: November 2019

AbstractBackgroundAlthough thyroid dysfunction caused by Hashimoto’s thyroiditis (HT) is believed to be related to implantation failure due to the underdevelopment of the receptive uterus, it is unknown whether HT itself, even in the euthyroid state, impairs embryo implantation associated with endometrial receptivity defects. To address whether HT itself can affect endometrial receptivity accompanied by implantation alterations, a euthyroid HT model was established in mice.MethodsFemale NOD mice were immunized twice with thyroglobulin and adjuvant to induce the experimental HT model. Four weeks after the second treatment, the mice were normally mated, and pregnant ones were sacrificed in implantation window for thyroid-related parameter and steroid hormones measurements by electrochemiluminescence immunoassay and enzyme-linked immunosorbent assay and implantation site number calculation by uptake of Chicago Blue dye. In addition, certain morphological features of endometrial receptivity were observed by hematoxylin-eosin staining and scanning electron microscopy, and the expression of other receptivity markers were analyzed by immunohistochemistry, RT-qPCR or Western Blot.ResultsHT mice displayed intrathyroidal monocyte infiltration and elevated serum thyroid autoantibody levels without thyroid dysfunction, defined as euthyroid HT in humans. Euthyroid HT resulted in implantation failure, fewer pinopodes, retarded pinopode maturation, and inhibited expression of receptivity markers: estrogen receptor α (ERα), integrin β3, leukemia inhibitory factor (LIF), and cell adhesion molecule-1 (ICAM-1). Interestingly, despite this compromised endometrial receptivity response, no statistical differences in serum estradiol or progesterone level between groups were found.ConclusionsThese findings are the first to indicate that HT induces a nonreceptive endometrial milieu in the euthyroid state, which may underlie the detrimental effects of HT itself on embryo implantation.