bluuebunny/crossref_metadata_2025_split huggingface dataset:
Faktor Risiko Yang Berpengaruh Pada Kejadian Tuberkulosis dengan Multidrug-Resistant Tuberculosis (MDR-TB) di RSUD Ulin Banjarmasin
Authors: Mashidayanti Aulia , Nurlely Nurlely , Kartinah Nani 
Published: November 2020

MDR-TB (Multidrug-Resistant Tuberculosis) adalah salah satu jenis TB yang resisten dengan OAT (Obat Anti Tuberculosis) dengan resisten terhadap 2 obat anti tuberculosis yang paling ampuh yaitu rifampisin dan isoniazid. Obat rifampisin dan isoniazid sudah tidak efektif dalam membunuh kuman mycobacterium tuberkulosis dikarenakan kuman yang sudah resisten terhadap obat tersebut. MDR-TB merupakan suatu permasalahan yang menjadi hambatan utama dunia dalam pemberantasan TB. Tujuan dari penelitian ini adalah untuk mengidentifikasi faktor risiko apa saja yang dapat berpengaruh pada kejadian tuberkulosis dengan multidrug-resistant tuberculosis (MDR-TB) di RSUD Ulin Banjarmasin dengan variabel yang ditinjau adalah pengetahuan, motivasi dan keteraturan minum obat. Metode penelitian dengaan rancangan Cross Sectional dengan metode pengambilan dengan kuesioner. Populasi dalam penelitian ini adalah seluruh pasien dengan diagnosis tuberkulosis multidrug resistant tuberculosis (MDR-TB) dan pasien TB Non MDR yang digunakan sebagai pembanding yang dipilih secara acak. Hasil pada penelitian ini menunjukkan bahwa faktor risiko yang terbukti berpengaruh pada kejadian TB-MDR adalah keteraturan minum obat (p-value< 0,05). Oleh karena itu, untuk mengurangi potensi bertambahnya penderita TB-MDR, maka perlu diperhatikan lagi keteraturan minum obat penderita, memastikan agar penderita benar-benar rutin dan teratur dalam minum obat. MDR-TB (Multidrug-Resistant Tuberculosis) is one of tuberculosis characterized by resistant to anti-TB drug (Anti Tuberculosis Drug). An MDR-TB event is a resistance event to 2 of the most effective anti-TB drugs which are rifampicin and isoniazid. Rifampicin and isoniazid are no longer effective in killing Mycobacterium tuberculosis bacteria due to its resistant to the drug. The purpose of this study is to identify any risk factors that can affect the incidence of tuberculosis with multidrug-resistant tuberculosis (MDR-TB) in RSUD Ulin Banjarmasin. The variables in this study were knowledge, motivation and regularity of taking drugs. The research method was a cross sectional design using questionnaire to the patients. The population in this study was all patients with a diagnosis of multidrug resistant tuberculosis (MDR-TB) and non-MDR TB patients who used as a comparison which were selected randomly. The results of this study indicate that the risk factor that has been shown to influence the incidence of MDR-TB was the regularity of taking medication (p-value <0.05). Therefore, to reduce the potential of MDR-TB sufferers to increase, it is necessary to pay attention to taking drug regularity of patient, ensuring that the patient is really routine and taking medication regularly.Keywords: RSUD Ulin Banjarmasin, MDR-TB (Multidrug-Resistant Tuberculosis), Tuberculosis
Gambaran Informasi dan Tindakan Petugas Kesehatan Terhadap Kepatuhan Berobat Pasien Tuberkulosis di Puskesmas Wilayah Kabupaten Banjar
Authors: Aprini Kesty , Nurlely Nurlely , Kartinah Nani 
Published: November 2020

Petugas kesehatan mempunyai pengaruh terhadap kualitas pelayanan kesehatan, termasuk pelayanan kesehatan terhadap pasien TB yang akan berpengaruh terhadap kepatuhan berobat. Tujuan penelitian ini menentukan persentase petugas kesehatan kategori baik, cukup, kurang dalam memberikan informasi dan tindakan terhadap pasien tuberkulosisdi Kabupaten Banjar. Penelitian ini dilakukan terhadap 97 petugas kesehatan dengan pendekatan cross sectional menggunakan kuesioner. Subjek pada penelitian ini yaitu tim program TB (Dokter, Perawat, Petugas lab) dan Apoteker/AA. Penilaian dilakukan terhadap karakteristik petugas, informasi dan tindakan yang diberikan petugas kesehatan terhadap kepatuhan berobat pasien TB yaitu dikategorikan baik, cukup dan kurang. Hasil penelitian pada karakteristik petugas kesehatan yaitu jenis kelamin perempuan sebanyak 68 orang, kategori umur >30 tahun sebanyak 57 orang, pendidikan terakhir yang paling banyak yaitu D3 sebanyak 47 orang, masa jabatan sebagai petugas TB (perawat) yang paling banyak yaitu selama <5 tahun sebanyak 20 orang. Informasi yang diberikan oleh petugas kesehatan terhadap kepatuhan berobat pasien TB dalam kategori baik yaitu 61 (62,88%) petugas kesehatan dan tindakan petugas TB sebanyak 47 (48,46%) petugas kesehatan. Oleh karena itu petugas kesehatan perlu memperhatikan lagi terkait kinerja pelayanan kesehatan agar informasi dan tindakan terhadap kepatuhan berobat pasien TB jauh lebih baik Health workers have an influence on the quality of health services, including health services for TB patients which will affect treatment compliance. The purpose of this study was to determine percentage of health workers in good, sufficient, and inadequate categories in providing information and action on tuberculosis patients in Banjar District. This research was conducted on 97 health workers with a cross sectional approach using a questionnaire. The subjects in this study were TB team programs which consist of Doctors, Nurses, Lab Officers and Pharmacists/Pharmacist assistant. An assessment was made of staff characteristics, information and actions given by health workers to TB patient treatment was categorized as good, sufficient and lacking. Thye result of this study characteristics on health workers are female sex which were 68 people, age categories>30 years as many as 57 people, the most recent education is D3 which were 47 people, while the longest term as TB officers (nurses) was for less than 5 years which were 20 people. Information provided by health workers on TB patient compliance in the good category was 61 (62.88%) health workers and the actions of TB workers were 47 (48.46%) health workers. Therefore, it is necessary to pay more attention to the performance of health services so that information and actions on TB patient compliance more better Keywords: TB Health care workers, Tuberculosis, Information, Action
WITHDRAWN: Efficacy and Safety of Ivermectin for Treatment and prophylaxis of COVID-19 Pandemic
Authors: Elgazzar Ahmed , Hany Basma , Youssef Shaimaa Abo , Hafez Mohy , Moussa Hany , eltaweel Abdelaziz 
Published: November 2020

Abstract

Background: Up-to-date, there is no recognized effective treatment or vaccine for the treatment of COVID-19 that emphasize urgency around distinctive effective therapies. This study aims to evaluate the anti-parasitic medication efficacy "Ivermectin" plus standard care ((Azithromycin, vitamin C, Zinc, Lactoferrin & Acetylcystein & prophylactic or therapeutic anticoagulation if D-dimer > 1000)) in the treatment of mild/moderate and severely ill cases with COVID 19 infection, as well as prophylaxis of health care and/ or household contacts in comparison to the Hydroxychloroquine plus standard treatment.Subject and methods: 600 subjects; 400 symptomatic confirmed COVID-19 patients and 200 health care and household contacts distributed over 6 groups; Group I: 100 patients with mild/moderate COVID-19 infection received a 4-days course of Ivermectin plus standard of care; Group II: 100 patients with mild/moderate COVID-19 infection received hydroxychloroquine plus standard of care; Group III: 100 patients with severe COVID-19 infection received Ivermectin plus standard of care; Group IV: 100 patients with Severe COVID-19 infection received hydroxychloroquine plus standard of care. Routine laboratory investigations and RT-PCR, were reported before and after initiation of treatment. Group V stick to PPE plus Ivermectin 400mcg / kg on empty stomach to be repeated after one week, and group VI stick to PPE only (personal protective equipment) .Both groups V&VI were followed for two weeks ..Results: Patients received ivermectin reported substantial recovery of laboratory investigations; and significant reduction in RT-PCR conversion days. A substantial improvement and reduction in mortality rate in Ivermectin treated groups; group I (mild/moderate cases), (99%, and 0.0%, respectively) and group III (severe cases), (94%, and 2.0% respectively) versus hydroxychloroquine plus standard care treated groups; group II (mild/moderate cases), (74% and 4%, respectively) and group IV (severe cases) (50% and 20%, respectively). Ivermectin had significantly reduced the incidence of infection in health care and household contacts up to 2% compared to 10% in non ivermectin group when used as a prophylaxis.Conclusion: Addition of Ivermectin to standard care is very effective drug for treatment of COVID-19 patients with significant reduction in mortality compared to Hydroxychloroquine plus standard treatment only. Early use of Ivermectin is very useful for controlling COVID 19 infections; prophylaxis and improving cytokines storm
A Pig White Matter Atlas and Common Connectivity Space Provide a Roadmap for the Introduction of a New Animal Model in Translational Neuroscience
Authors: Benn R. Austin , Mars Rogier , Xu Ting , Rodríguez-Esparragoza Luis , Montesinos Paula , Manzano-Patron J.P , Lopez-Martin Gonzalo , Fuster Valentin , Sánchez-González Javier , Duff Eugene , Ibanez Borja 
Published: November 2020

Abstract
The characterization and definition of homology in the cerebral cortex needed for a species to be adopted as a translational model in neuroscience is a unique challenge given the diverse array of cortical morphology present in the mammalian lineage. Using the domestic pig as an example, we provide a roadmap of how leveraging Magnetic Resonance Imaging of the brain and data-driven tractography can overcome these obstacles and facilitate cortical alignment between distantly related species. In doing so, we created a full platform of neuroimaging tools to be used in the pig, including volumetric and surface templates, a structural white matter atlas, and the establishment of a common connectivity space to facilitate pig-human cortical alignment. Releasing our data and code and our pig-human cortical alignment, we permit researchers already working with the pig to accentuate the clinical relevance and translational capacity of their work. By sharing the intermediate outputs and scripts used to construct our pig-human cortical alignment, we also provide a roadmap to expand the current repertoire of animal models used in neuroscience.
p38β - MAPK11 and its role in female cancers
Authors: Katopodis Periklis , Kerslake Rachel , Zikopoulos Athanasios , Beri Nefeli Eirini , Anikin Vladimir 
Published: November 2020

Abstract
Background The p38MAPK family of Mitogen Activated Protein Kinases are a group of signalling molecules involved in cell growth, survival, proliferation and differentiation. The widely studied p38α isoform is ubiquitously expressed and is implicated in a number of cancer pathologies, as are p38γ and p38δ. However, the mechanistic role of the isoform, p38β, remains fairly elusive. Recent studies suggest a possible role of p38β in both breast and endometrial cancer with research suggesting involvement in bone metastasis and cancer cell survival. Female tissue specific cancers such as breast, endometrial, uterine and ovary account for over 3,000,000 cancer related incidents annually; advancements in therapeutics and treatment however require a deeper understanding of the molecular aetiology associated with these diseases. This study provides an overview of the MAPK signalling molecule p38β (MAPK11) in female cancers using an in-silico approach. Methods A detailed gene expression and methylation analysis was performed using datasets from cBioportal, CanSar and MEXPRESS. Breast, Uterine Endometrial, Cervical, Ovarian and Uterine Carcinosarcoma TCGA cancer datasets were used and analysed.Results Data using cBioportal and CanSAR suggest that expression of p38β is lower in cancers: BRCA, UCEC, UCS, CESC and OV compared to normal tissue. Methylation data from SMART and MEXPRESS indicate significant probe level variation of CpG island methylation status of the gene MAPK11. Analysis of the genes’ two CpG islands shows that the gene was hypermethylated in the CpG1 with increased methylation seen in BRCA, CESC and UCEC cancer data sets with a slight increase of expression recorded in cancer samples. CpG2 exhibited hypomethylation with no significant difference between samples and high levels of expression. Further analysis from MEXPRESS revealed no significance between probe methylation and altered levels of expression. In addition, no difference in the expression of BRCA oestrogen/progesterone/HER2 status was seen. Conclusion This data provides an overview of the expression of p38β in female tissue specific cancers, showing a decrease in expression of the gene in BRCA, UCEC, CESC, UCS and OV, increasing the understanding of p38β MAPK expression and offering insight for future in-vitro investigation and therapeutic application.
Role of doxycycline, oral steroids, and nasal steroid in treatment of anosmia due to COVID-19 with the new insights into the doxycycline activity.
Authors: Husain Parvez , Ahmed Shaheen Khalil , Husain Benazeer 
Published: November 2020

Abstract
Background All over the world, severe Acute Respiratory Syndrome coronavirus-2 (SARS-CoV-2) has been the most important public health issue this year; recently this was re-named as corona virus disease 2019 (COVID-19) by the World Health Organization (WHO). Olfactory or gustatory dysfunction is often reported as a symptom.Aims Aim of the study is to evaluate the role and effectiveness of Doxycycline, Oral Methyl Prednisolone, and Topical Nasal Steroid (Mometasone) in the treatment of COVID-19 related anosmia/hyposmiaMaterial and Methods Total 64 patients were evaluated randomly who were COVID-19 positive and developed recent onset of anosmia/hyposmia. They were divided into two groups 32 each. First group was treated with Doxycycline, Oral Methyl Prednisolone, and Topical Nasal Steroid (Mometasone). Second group was the control group and was given placebo and Olfactory training.Results Out of 32 patients 27 patients completely recover after only 2 days of the administration of above treatment. Out of the remaining 5 patients 4 had partial resolution of the anosmia after 2 days and near complete resolution after 5-7 days, and 1 patient did not responded at all with the above treatment. Out of the control group 19 patient had near complete recovery after 7 days and rest 13 patient did not recover after 10 days.Conclusion The above study showed a very promising results but many more similar studies are needed following the similar treatment protocol on a larger group of patients suffering from olfactory dysfunction.
Rapid Serological Tests for SARS-COV-2: Diagnostic Performance of Four Commercial Assays
Authors: de Almeida Sérgio Monteiro , Spalanzani Regiane Nogueira , Nogueira Meri Bordignon , Spiri Beatriz Sanada , Cavalli Barbara Maria , Rotta Indianara , Takahashi Gislene Reche de Almeida , Pereira Luciane Aparecida , Moreira Francielli Brusco , Dino Carolina Lumi Tanaka , Graf Maria Esther , de Almeida Bernardo Montesanti Machado , Domino Natalia Ramos , Genelhoud Gustavo , Bueno Lucas Bochnia , Raboni Sonia Mara 
Published: November 2020

Abstract
Background. This study aimed to assess the diagnostic performance of lateral flow immunochromatographic assays (LFA) of four different manufacturers to identify SARS-CoV-2 antibodies (IgM, IgG or total), comparing them with the nucleic acid amplification test (NAAT) or clinical defined (definite or probable SARS-CoV-2 infection respectively). Methods. 119 serum samples were randomly selected by convenience and distributed in the groups: (1) Group with SARS-CoV-2 infection [n=82; RT-qPCR positive (definite, n=70), and probable (n=12)]; (2) other diseases [n= 27; other viruses identified (n=8), SARS of other etiologies (n=19)]; (3) healthy control group (n=10). LFA essays of four manufacturers were compared: MedTest Coronavírus (COVID-19) IgG/IgM (MedLevensohn, Brazil); COVID-19 IgG/IgM ECO Test (Ecodiagnóstica, Brazil); Camtech COVID-19 IgM/IgG Rapid Test Kit (Camtech Diagnostics Pte Ltd, Singapore); and one Step COVID-19 Test for total antibodies (Guangzhou Wondfo Biotech Co, China).Results. The four tests studied showed high diagnostic performance characteristics for the diagnoses of definite or probable SARS-CoV-2 infection. The best measures were for the Wondfo test: sensitivity (86.59%; 95%CI, 77.26-93.11%); specificity (100%; 90.51-100%); DOR (257; 60-1008); LR+ (33.43; 4.82-231.85); LR− (0.13; 0.08 - 0.23); accuracy (90.76%; 84.06- 95.29%); Matthews Correlation coefficient (MCC) 0.82. Although considering only the probable SARS-CoV-2 infection (PCR-) cases, all the kits studied showed limited values.Conclusion. Our data demonstrate the excellent performance of LFA for the diagnoses of definite or probable SARS-CoV-2 infection. There was substantial heterogeneity in sensitivities of IgM and IgG antibodies among the manufacturers. LFA tests cannot replace molecular diagnostics, but should be used as additional screening tool.
Long Non-coding RNA LINC02085 Mediates Cell Growth and Inflammatory Response of Fibroblast-Like Synoviocytes by Activating PI3K/AKT Signals in Rheumatoid Arthritisis
Authors: Wen Jianting , Liu Jian , Wang Xin , Wang Jie 
Published: November 2020

Abstract
Background: The present study explored the possible functions and the underlying mechanism of Long Non-coding RNA LINC02085 in rheumatoid arthritis (RA). Methods: Peripheral blood mononuclear cells (PBMCs) were collected from patients with RA. Primary fibroblast-like synoviocytes (FLS) were separated from synovial tissues and was established cell lines, then cultured for subsequent cell experiments by transfecting different vectors. The RT-qPCR analysis was employed for evaluating the levels of LINC02085 in the PBMCs and RA-FLS. ELISA analysis was employed to detect the levels of inflammatory cytokines. CCK8 assay, migration and invasion assays were used to evaluate the proliferation, migration and invasion abilities of cells, respectively. Besides, the levels of the the PI3K/AKT pathway-related proteins were measured by WB and IF. Spearman correlation analysis, association rule analysis, logistic regression analysis were used to assess the correlation between LINC02085 and clinical parameters. Results: The expression level of LINC02085 was significant high in patients with RA, and positively associated with age, ESR, CRP, RF, CCP and DAS28. Moreover, logistic regression analysis indicated that ESR, CRP, RF and DAS28 were risk factors for LINC02085. We found that LINC02085 was upregulated in RA -FLS and TNF-αstimulated. And overexpression of LINC02085 could promote proliferation, migration and invasion induced by TNF-α, through upregulating the levels of TNF-αand TNFAIP2 and promoting the activation of PI3K/AKT pathway. Whereas knockdown of LINC02085 received the opposite results. ﻿Conclusion: In conclusion, the present study revealed that LINC02085 could regulate cell growth and inflammatory response of RA-FLS by activating the PI3K/ AKT signaling pathway, subsequently playing important roles in promoting the occurrence and development of RA.
APEX1 regulates alternative splicing of key tumorigenesis genes in non-small-cell lung cancer
Authors: Peng Li , Liu Yuwei , Chen Jing , Du Xuebei , Zhang Renwei , Cheng Mengxin , Wu Ying , Chen Min , Zhong Ya , Shen Dan , Chen Ling , Ye Xujun 
Published: November 2020

Abstract
Background: Aberrant alternative splicing (AS) contributes to tumor progression. Previous studies have shown that apurinic-apyrimidinic endonuclease-1 (APEX1) is involved in tumor progression. It is unknown whether APEX1 functions in tumor progression by regulation of AS. It is also unknown whether APEX1 can regulate non-small-cell lung cancer (NSCLC) proliferation and apoptosis. Methods: We analyzed APEX1 expression levels in 517 lung NSCLC samples from the TCGA (Cancer Genome Atlas) database. The impact of APEX1 over expression on A549 cell proliferation and apoptosis was detected by the methyl thiazolyl tetrazolium assay and by flow cytometry. The transcriptome of A549 cells with and without APEX1 over expression was determined by Illumina sequencing, followed by analysis of AS. RT-qPCR validated APEX1 in A549 cells. Results: We have successfully applied RNA-seq technology to demonstrate APEX1 regulation of AS. APEX1 expression was shown to be upregulated in NSCLC samples and to reduce cell proliferation and induce apoptosis of A549 cells. Further, APEX1 regulated AS of key tumorigenesis genes involved in cancer proliferation and apoptosis within the MAPK and Wnt signaling pathways. Each of these pathways are involved in lung cancer progression. Validated AS events regulated by APEX1 were located in key tumorigenesis genes; AXIN1 (axis inhibition protein 1), GCNT2 (N-acetyl glucosaminyl transferase 2), and SMAD3 (SMAD Family Member 3). These genes encode signaling pathway transcription regulatory factors. Conclusions: We found that increased expression of APEX1 in NSCLC is an independent prognostic factor related to tumor progression. Therefore, APEX1 regulation of AS may serve as a molecular marker or therapeutic target for NSCLC treatment.
Lightweight Model For The Prediction of COVID-19 Through The Detection And Segmentation of Lesions in Chest CT Scans
Authors: Ter-Sarkisov Aram 
Published: November 2020

Abstract
We introduce a lightweight Mask R-CNN model that segments areas with the Ground Glass Opacity and Consolidation in chest CT scans. The model uses truncated ResNet18 and ResNet34 nets with a single layer of Feature Pyramid Network as a backbone net, thus substantially reducing the number of the parameters and the training time compared to similar solutions using deeper networks. Without any data balancing and manipulations, and using only a small fraction of the training data, COVID-CT-Mask-Net classification model with 6.12M total and 600K trainable parameters derived from Mask R-CNN, achieves 91.35% COVID-19 sensitivity, 91.63% Common Pneumonia sensitivity, 96.98% true negative rate and 93.95% overall accuracy on COVIDx-CT dataset (21191 images). We also present a thorough analysis of the regional features critical to the correct classification of the image. The full source code, models and pretrained weights are available on https://github.com/AlexTS1980/COVID-CT-Mask-Net.
A simulation is worth a thousand worlds - Importance sampling Monte Carlo applied to COVID-19 contingency
Authors: Rapuzzi Andrea , Vairo Tomaso 
Published: November 2020

Abstract
COVID-19 outbreak has become a global pandemic that affected more than 200 countries worldwide. Predicting the behavior of this outbreak has a crucial role in organizing preventive and protective actions, and in improving the decision making process.The aim of predicting the number of people who contract the virus has so far been pursued with regression models (exponential, logistics, ...), but regressions can integrate the variable context only a posteriori. The regression models are all dependent on their own history, thus, they can not display anything which did not happen before.The pandemic infection of COVID-19 presents a transmission behavior that is widely changing over time. This is due to the growth of the efficiency in the detection of infected, for the changes in social distancing measures and for the widespread use of individual protection devices.The approach presented in this paper, starting from the definition of simplified risk assessment framework, aims at designing a probabilistic model for the virus transmission and detection, keeping into account this context changes, binding the correct set of variables to them, and at inferring the distribution for the underlying stochastic variables. This is a key to unlock innovative and valuable insights from the current events. The model has been built in Gen, a probabilistic programming system, built at MIT and embedded in Julia.