bluuebunny/crossref_metadata_2025_split huggingface dataset:
A novel scoring system for the prediction of disease severity in STEC‐HUS
Authors: Ishibazawa Emi , Nagamori Tsunehisa , Kurisawa Mio June , Sato Masayuki , Yoshida Yoichiro , Takahashi Hironori , Manabe Hiromi , Ishioka Toru , Miura Yurika , Kajino Hiroki , Suzuki Yasuto , Wada Soichiro , Ogiwara Shigetoshi , Tomii Yuji , Aoyagi Hayato , Nagai Kazushige , Naito Hiroyuki , Takahashi Satoru 
Published: December 2024

AbstractBackgroundShiga toxin‐producing Escherichia coli‐associated hemolytic uremic syndrome (STEC‐HUS) is a life‐threatening condition complicated by acute kidney injury, acute respiratory distress syndrome, and central nervous system disorders. The early identification of high‐risk patients is required to facilitate timely and appropriate treatment.MethodsThe medical records of patients with STEC‐HUS treated at 11 hospitals in Hokkaido, Japan, were reviewed retrospectively. A multi‐institutional retrospective analysis was performed in which patients were divided into two groups according to the presence or absence of severe complications requiring blood purification therapy or encephalopathy. We compared the laboratory values at diagnosis between the severe and mild groups. To identify patients at high risk of developing severe complications, a scoring system, referred to as the “STEC‐HUS severity (STEC‐HUSS) score,” was constructed based on the parameters showing significant differences.ResultsOf the 41 patients with STEC‐HUS, 11 were classified into the severe group and 30 into the mild group. Significant differences were observed between the groups in terms of white blood cell count, activated partial thromboplastin time, fibrinogen, D‐dimer, total protein, aspartate transaminase, alanine transaminase, lactate dehydrogenase, creatinine, and C‐reactive protein levels. The STEC‐HUSS score was calculated on a scale of 0–10 by summing the number of test items that demonstrated abnormal values. The STEC‐HUSS score, when the cut‐off value was 4, showed a sensitivity of 100% and a specificity of 91% in the severe group.ConclusionWe developed a novel scoring system to identify patients at high risk of severe STEC‐HUS.
Preprocessing choices for P3 analyses with mobile EEG: A systematic literature review and interactive exploration
Authors: Jacobsen Nadine S. J. , Kristanto Daniel , Welp Suong , Inceler Yusuf Cosku , Debener Stefan 
Published: December 2024

AbstractPreprocessing is necessary to extract meaningful results from electroencephalography (EEG) data. With many possible preprocessing choices, their impact on outcomes is fundamental. While previous studies have explored the effects of preprocessing on stationary EEG data, this research delves into mobile EEG, where complex processing is necessary to address motion artifacts. Specifically, we describe the preprocessing choices studies reported for analyzing the P3 event‐related potential (ERP) during walking and standing. A systematic review of 258 studies of the P3 during walking, identified 27 studies meeting the inclusion criteria. Two independent coders extracted preprocessing choices reported in each study. Analysis of preprocessing choices revealed commonalities and differences, such as the widespread use of offline filters but limited application of line noise correction (3 of 27 studies). Notably, 59% of studies involved manual processing steps, and 56% omitted reporting critical parameters for at least one step. All studies employed unique preprocessing strategies. These findings align with stationary EEG preprocessing results, emphasizing the necessity for standardized reporting in mobile EEG research. We implemented an interactive visualization tool (Shiny app) to aid the exploration of the preprocessing landscape. The app allows users to structure the literature regarding different processing steps, enter planned processing methods, and compare them with the literature. The app could be utilized to examine how these choices impact P3 results and understand the robustness of various processing options. We hope to increase awareness regarding the potential influence of preprocessing decisions and advocate for comprehensive reporting standards to foster reproducibility in mobile EEG research.
Integrated Quantitative Proteomics and Phosphoproteomics Analysis Reveals the Dynamic Process of Buffalo (Bubalus bubalis) Spermatogenesis
Authors: Zhang Pengfei , Wang Chenyang , Liu Xuyang , Zhang Ming , Fu Qiang , Pan Limei , Huang Yulin 
Published: December 2024

ABSTRACTSpermatogenesis is a highly complex and tightly regulated cellular differentiation process closely related to the productive performance of male livestock. We do not yet have a clear understanding of the spermatogenesis mechanism of buffalo. In this study, spermatogonia, spermatocytes and spermatids were analysed by flow cytometry. Quantitative proteomic and phosphoproteomic studies were performed on different spermatogenic cells using tandem mass tagging technology and liquid chromatography–tandem mass spectrometry. A total of 219 differentially expressed proteins (involved in focal adhesions and actin cytoskeleton pathways) and 71 phosphoproteins (involved in RNA transport and adhesion junction pathways) were obtained. Through trend analysis, a dynamic profile of protein expression was obtained, enriched to the main biological processes at different stages of spermatogenesis. By immunohistochemical localisation analysis, it was found that MACROH2A2, TOP2A, LMNA, LMNA (pS392), VIM and VIM (pS56) had specific localisation in testis cells. Network analysis of kinase‐substrate phosphorylation sites showed that AKT1 is the most active kinase, LMNA is regulated by most kinases and AKT1 can catalyse the phosphorylation of LMNA. This study provides a reference for studying the molecular mechanism of buffalo spermatogenesis and helps clarify the regulatory mechanism of protein translation and post‐translational modification during mammalian spermatogenesis.
Critical soil organic carbon for improving available water content of silt loam soils
Authors: Li Xinhu , Feng Gary , Tewolde Haile , Adeli Ardeshir , Jenkins Johnie N. 
Published: December 2024

AbstractThe effect of soil carbon on the available water content (AWC) has garnered considerable attention. An increasing number of studies have recognized a beneficial impact of soil organic matter (SOM) on available water content (AWC); however, results are not consistent regarding the magnitude of soil organic carbon (SOC) effect on AWC. In particular, the critical SOC range necessary for enhancing AWC requires clarification. Accordingly, 165 samples were collected from four sites, where two sites received poultry litter (PL) and two sites did not, covering a wide range of carbon contents from low to high (4.5–22.9 g kg−1). The bulk density was the same, and the soils were tested for their field capacity (FC) and permanent wilt point (PWP) in the lab. The results revealed that there was no significant correlation between the clay and AWC, and a significant positive relationship between SOC and AWC was exhibited, suggesting that the increase in AWC mostly resulted from the increase in SOC. However, the AWC did not change significantly with increasing SOC when the SOC was below 10 g kg−1, whereas a significant increase in AWC occurred when the SOC increased from 10 to 14 g kg−1. The largest increment in AWC occurred when the SOC increased from 14 to 18 g kg−1, but the increase in AWC with increasing SOC decreased when the SOC was above 18 g kg−1. Thus, a critical range of SOC for AWC from 14 to 18 g kg−1 could significantly improve the soil water‐holding capacity. Additionally, most soil samples within the critical SOC range were collected from sites that received PL. Thus, this study also evaluated the enhancement of AWC by PL application, which efficiently improved the soil water‐holding capacity.
Impact of postoperative radiography on the management of humeral condylar fractures in immature dogs
Authors: Karydas Stavros , Quinonero Ignacio , Chitty Jake , Malucelli Davide , Solano Miguel , Vallefuoco Rosario , Danielski Alan 
Published: December 2024

AbstractObjectiveTo evaluate the impact of postoperative radiographs on treatment decisions for skeletally immature dogs undergoing repair of a fractured humeral condyle.Study designRetrospective observational multicenter study.Sample populationA total of 139 dogs (<12 months of age).MethodsMedical records of dogs who underwent humeral condylar fracture repair were retrieved and analyzed. Data collected included dog's signalment, fixation method, reported client's concerns, follow‐up clinical examination findings, radiographic assessment, and any documented changes to the postoperative plan.ResultsOut of the 139 cases, 23 dogs (17%) required a change in their postoperative plan. Owner concerns (OR: 7.6), prolonged use of analgesic drugs (OR: 7.9), presence of lameness (OR: 5.9), abnormal clinical findings at the time of follow‐up examination (OR: 44.8) and radiographic abnormalities (OR: 51.9) significantly increased the likelihood of a change in the postoperative plan. Most importantly, none of these postoperative plan changes were solely attributed to radiographic abnormalities at the time of the follow‐up examination.ConclusionFollow‐up radiographs did not influence the postoperative management plan in cases where clients reported no concerns, the dogs did not require prolonged analgesic treatment, showed no lameness on examination, and had unremarkable clinical findingsClinical significanceIn the absence of client concerns, prolonged use of analgesic drugs, lameness on examination, and clinical abnormalities, the necessity of follow‐up radiographs in immature dogs that underwent humeral condylar fracture repair is debatable.
Draft genome sequences of
Pseudomonas chengduensis
strain BW1 and
Sphingobium
sp. strain MK2 isolated from oil sands process-affected water
Authors: Anuforo Philemon Chinemezu , Würz Birgit , Wick Lukas Y. , Kallies René 
Published: December 2024

ABSTRACT

Draft genomes of two phenanthrene-degrading bacterial isolates from oil sands process-affected water (OSPW) in Alberta, Canada were sequenced. Both isolates grew in close association on agar plates and were difficult to obtain axenically. They represent novel
Pseudomonas chengduensis
and
Sphingobium
sp. strains with genomes of 5.5 and 4.1 Mbases length, respectively.
Synthesis of New 1-(4-Anilinophenyl)-1H-pyrrole-2,5-diones
Authors: Kolyamshin O. A. , Mitrasov Yu. N. , Danilov V. A. , Pylchikova Yu. Yu. 
Published: December 2024

Abstract
The reaction of N1-phenyl- and N1-(4-methoxyphenyl)benzene-1,4-diamines with maleic anhydride gave the corresponding maleic acid monoamides whose cyclization in the presence of p-toluenesulfonic acid afforded 1-[4-anilino- and 4-(4-methoxyanilino)phenyl]-1H-pyrrole-2,5-diones.
Ag+/Li+ Ion-Exchange in LLZTO to Introduce Surface Layer Compressive Stress and Improve Electrochemical Performance
Authors: Morris Sydney , Jagad Harsh , Harris Stephen J , Shi Changmin , Qi Yue , Sheldon Brian W. 
Published: December 2024

Ion-exchange (IX) is frequently used in the manufacturing of glasses to increase their fracture resistance. Using IX to introduce surface layer compressive stress in a solid electrolyte like lithium lanthanum zirconium tantalum oxide (LLZTO) has the potential to improve electrochemical performance by delaying lithium (Li) filament penetration through the solid electrolyte (SE). Computational work has proven the feasibility of ion-exchange between lithium ions and larger ions in LLZTO depending on the precursor used [1]. This experimental work explores several salt chemistries and heat treatments to evaluate the impact of IX on LLZTO. A multi-beam optical stress sensor (MOSS) was used to measure changes in curvature from IX induced stress. Scanning electron microscope (SEM) and electron-dispersive X-ray spectroscopy (EDS) were used to characterize the composition of samples. Symmetric Li/Li cells were used to measure critical current density (CCD) and for long-term cycling tests to benchmark improvements in electrochemical performance. Results show controlled IX with LLZTO can introduce stress in the near surface layer, increase CCDs, and improve long-term cycling performance in a symmetric Li/Li cell.
[1] Tradeoff between the Ion Exchange-Induced Residual Stress and Ion Transport in Solid Electrolytes. Harsh D. Jagad, Stephen J. Harris, Brian W. Sheldon, and Yue Qi. Chemistry of Materials 2022 34 (19), 8694-8704. DOI: 10.1021/acs.chemmater.2c01806
Complementary and Hybrid Approaches for Enhanced Solar Utilization in Synthetic Fuel Production
Authors: Pornrungroj Chanon 
Published: December 2024

The synthesis of fuels from sunlight offers a promising sustainable solution for chemical energy storage. However, the inefficient utilisation of the solar spectrum limits its commercial viability. Conventional solar fuel synthesis relies heavily on high-energy photons from the ultraviolet range, leaving much of the solar spectrum, particularly visible and infrared energies, underutilized. This results in significant energy loss as unabsorbed light is converted into wasted heat, reducing overall efficiency.1 In this work, we propose strategies to enhance solar energy utilization by implementing advanced thermal management and integrating both complementary and hybrid processes. Thermal management techniques are designed to capture and repurpose heat generated by unutilized visible and infrared light, converting it into useful energy and improving system efficiency.2,3 Additionally, hybrid systems that combine solar fuel synthesis with water purification using photocatalysis and photothermal processes are also explored. These hybrid systems can harness a broader spectrum of solar energy, directly converting it into chemical energy and clean water, thereby increasing solar utilization.4 By addressing these inefficiencies through a combination of thermal management, complementary process integration, and material and architectural innovation, this work provides a comprehensive framework for enhancing the viability of solar fuel production. These strategies underscore the importance of interdisciplinary collaboration in advancing solar fuel technologies, paving the way for a sustainable energy economy and supporting the transition to a cleaner, more sustainable energy landscape.
References
1.Wang, Q.; Pornrungroj, C.; Linley, S.; Reisner, E., “Strategies to improve light utilization in solar fuel synthesis”, Nature Energy; 7, 13-24 (2022).
2.Pornrungroj, C.; Andrei, V.; Reisner, E., “Thermoelectric–photoelectrochemical water splitting under concentrated solar irradiation”, Journal of the American Chemical Society; 145, 13709-13714 (2023).
3.Cobb, S.J.; Pornrungroj, C.; Andrei, V.; Badiani, V.M.; Su, L.; Manuel, R.R.; Pereira, I.A; Reisner, E., “A photoelectrochemical-thermoelectric device for semi-artificial CO2 fixation employing full solar spectrum utilization”. Device (2024). https://doi.org/10.1016/j.device.2024.100505

4. Pornrungroj, C.; Annuar, A. B. M.; Wang, Q.; Rahaman, M.; Bhattacharjee, S.; Rahaman, M.; Andrei, V.; Reisner, E., “Hybrid photothermal–photocatalyst sheets for solar-driven overall water splitting coupled to water purification”, Nature Water; 1, 952-960 (2023).
CoO/Co3O4 Hybrid Oxide Anode Synthesis Techniques and Performance Improvement for Super-Theoretical Capacity in Lithium-Ion Batteries
Authors: Kumchompoo Jaturon , Lee Jyh-Tsung - 
Published: December 2024

The electrochemical instability of transition metal oxide (TMO)-based electrodes has hindered their broader adoption in lithium-ion batteries (LIBs), primarily due to rapid performance degradation. Addressing this challenge requires the development of TMO materials with enhanced lithium storage capacities and improved cycling stability. In this study, we report a facile hydrothermal synthesis of a CoO/Co₃O₄ hybrid oxide, followed by high-temperature calcination, aimed at boosting the electrochemical performance of these electrodes. Fluoroethylene carbonate (FEC) and vinylene carbonate (VC) additives were introduced to stabilize the Co₃O₄ interface, minimizing electrolyte decomposition and enhancing the electrode/electrolyte interface. At an optimized concentration of 2 wt%, these additives significantly improved cycling stability and capacity retention.
To further address common issues like grain boundary cracking and void formation, calcination at 800°C with 2% FEC was employed, resulting in improved Li⁺ transport and structural stability. This optimized synthesis and additive strategy led to remarkable improvements in the CoO/Co₃O₄ anode’s electrochemical performance, achieving a super-theoretical capacity (STC) phenomenon. After 400 cycles at 0.5 C, the CoO/Co₃O₄ anode exhibited an extraordinary capacity of 1427.35 mAh g⁻¹, a significant increase from its initial capacity of 925 mAh g⁻¹.
The observed STC is attributed to enhanced additive-driven electrolyte stabilization, the formation of a robust solid electrolyte interphase (SEI), and the mitigation of electrode surface cracking. Structural characterization via SEM, TEM, and BET analyses confirmed increased porosity and structural stability after prolonged cycling, further supporting the STC phenomenon. This study highlights the critical importance of tailored material synthesis and electrolyte engineering in overcoming the limitations of TMO-based anodes, paving the way for high-performance, long-lasting LIBs.

Figure 1
Developing Routes for 3D Printed Carbon Aerogel Electrodes
Authors: Worsley Marcus Andre 
Published: December 2024

Aerogels are porous solids used in a number of electrochemical applications including catalysis, batteries, and supercapacitors due to their high internal surface, composition, and small pore/particle size. Carbon electrodes (e.g. nanotubes, graphene, etc.) are particularly attractive due to their low electrical resistance. The efficient transport of charged species within energy storage devices, in particular, is critical to realizing both high power and energy densities, especially when operating under extreme conditions (e.g. ultralow temperatures, high charge/discharge rates, etc.). A number of resistances can contribute to sluggish ion/electron transport in a cell such as charge transfer at interfaces, electrolyte resistance, electrode resistance, and diffusive transport within porous electrodes. Several strategies have been pursued to address these transport limitations. Electrode materials discovery and development is a popular strategy as the charge storage mechanism and electrode resistance play a key role in kinetics of the device. Within electrode materials development, engineering of the electrode pore architecture is an extremely effective strategy to decrease the diffusive transport lengths and electrode resistances. This pore engineering can be achieved through synthetic chemistry for small pores (e.g. <10 um) and via additive manufacturing for larger pores (> 10 um). Here we show how electrode materials development can be used to overcome sluggish transport in aerogel-inspired electrodes. Particular focus will be given to the use of synthetic means to tune to the properties of electrode material (e.g. surface area, pore size, mechanical properties) and 3D printing to define the larger pore structure to improve the ultimate performance of the device. We explore a number of additive manufacturing techniques (e.g. direct ink write, projection microstereolithography, etc.) to print a variety of simple and complex structures to determine the optimal electrode architecture. The impact of these different methods on both aerogel properties and device function will be discussed.
This work was performed under the auspices of the U.S. Department of Energy by Lawrence Livermore National Laboratory under Contract DE-AC52-07NA27344.
(Invited) Parallelized Fabrication and Functionalization of Graphene Field-Effect Biosensors
Authors: Bouilly Delphine 
Published: December 2024

Graphene field-effect transistors (GFETs) are increasingly recognized for their potential as biosensors, due to their unique electrical and surface properties [1]. However, the difficulty to achieve high throughput and control in the fabrication and functionalization of GFETs remains a significant limitation, hindering the scalability and widespread adoption of these devices. In this presentation, we will discuss our recent work using parallelization and automation to develop innovative processes for the fabrication, functionalization and measurement of GFETs. First, we will introduce an automated method for the simultaneous transfer of arrays of graphene ribbons onto patterned wafers [2]. We developed a simple apparatus based on transfer columns and pumps to achieve the precise positioning and orientation of macroscopic graphene ribbons. Our method significantly reduces the time and materials consumption compared to traditional and large-scale transfer approaches, while maintaining an excellent quality of graphene. We will demonstrate the unique advantages of this approach to assemble arrays of multilayered devices. Second, we will present our approach using an electrolyte gating potential to dynamically modulate the functionalization of GFET arrays [3]. We will demonstrate the capacity of this approach to rapidly trigger, modulate and stop the reaction of graphene with aryldiazonium salts. Using a multiplexed electrical probing system interfaced with a flow cell, we will show that the unbiased execution of this chemistry results in heterogeneous and low yields of reaction on GFET arrays, whereas our technique results in a tunable and homogenous degree of functionalization across all devices. Finally, we will discuss how these approaches not only improve efficiency and control but also open new possibilities for the large-scale production of GFET arrays, and their interest for biosensing applications.
[1] A. Béraud et al., Analyst 146, 403-428 (2021)
[2] A. Bencherif et al., ChemRxiv. doi.org/10.26434/chemrxiv-2023-3ck5c (2023)
[3] C. M. Bazán et al., Nano Letters 22, 2635–2642 (2022)