bluuebunny/crossref_metadata_2025_split huggingface dataset:
LncRNA KCNK15-AS1 inhibits pancreatic cancer progression by promoting the expression of its binding protein ACTR3B
Authors: Wu Pengfei , Yuan Hao , Ding Xiangya , Chen Qun , Ge Wanli , Meng Lingdong , Huang Xumin , Shen Peng , Lu Yichao , Lu Zipeng , Jiang kuirong 
Published: August 2020

Abstract
Background

LncRNAs are reported to play an essential role in multiple tumors, including pancreatic cancer. LncRNAs could impact tumor growth via RNA-binding proteins, working as a coactivators of transcription factors or impacting their gene expression via posttranscriptional regulation. Our study aimed to elucidate the function and mechanism of lncRNA KCNK15-AS1 and its binding protein ACTR3B in PC progression. Our previous data indicated that KCNK15-AS1 is downregulated in PC tissues and cell lines compared to normal controls.
Methods

In this study, we overexpressed KCNK15-AS1 and ACTR3B in both BxPC-3 and Mia-PaCa-2 cells to detect the cellular phenotype in vitro and in vivo. RNA pulldown assays, mass spectrometry assays and RNA-binding protein immunoprecipitation assays were used to verify KCNK15-AS1 RNA binding protein ACTR3B. Luciferase reporter assay and ubiquitination assay were proceeded to detect the mechanism KCNK15-AS1 upregulated ACTR3B expression.
Results

Our results showed that overexpression of KCNK15-AS1 significantly inhibited the proliferation, colony formation and migration of PC cells. ACTR3B was screened by RNA pulldown and mass spectrometry assays. RNA-binding protein immunoprecipitation assays confirmed that KCNK15-AS1 physically bound to ACTR3B. Furthermore, mechanistic analyses demonstrated that KCNK15-AS1 promoted ACTR3B expression by inhibiting ACTR3B ubiquitin-mediated degradation and enhancing its promoter activity. Additionally, ACTR3B presented low expression in PC tissues and cell lines, and PC cell growth was significantly repressed when ACTR3B was overexpressed. Moreover, knockdown of ACTR3B in KCNK15-AS1-overexpressing cells reversed the effects of KCNK15-AS1 on PC cell growth via the cyclin D1/CDK4 axis.
Conclusion

Briefly, our study indicated that the lncRNA KCNK15-AS1/ACTR3B/cyclin D1/CDK4 axis may inhibit PC progression, which provides a potential therapeutic target for PC.
Evaluation the energy production potential of aggressively invasive Prosopis juliflora Sw (Fabacea): an emerging threat in the Bundala Ramsar Wetland
Authors: Bandara W.A.R.T.W. , Ranasinghe Oshadi Ruwanthika , Vlosky Richard 
Published: August 2020

Abstract
Prosopis juliflora is an invasive plant species rapidly expanding in the Asian and African continents. Invasion of P. juliflora in Bundala Ramsar Wetland (BRW) in Sri Lanka has created a number of biodiversity and conservation issues. This study was conducted to assess the possibility to utilize this invasive plant as a fuel source for local industries. The moisture content, wood density, ash content, volatile matter, fixed carbon content, biomass/ash ration and calorific value of P. juliflora were measured and compared with Leucaena leucocephala, which is a widely used fuelwood source in Sri Lanka and elsewhere. P. juliflora , performed better than L. leucocephala for most of these parameters. Ash content was comparatively higher in P. juliflora than that of L. leucocephala ; however, biomass to ash ratio of P. juliflora was significantly lower (at 0.05 level of significance) than that of L. leucocephala, suggesting its suitability as a fuelwood source. Further, the fuel value index of P. juliflora (3,276) was slightly lower than that of L. leucocephala (3,336), a non-significant difference. P. juliflora and L. leucocephala reached Fiber Saturation Point values within drying periods of 24 and 27 days, respectively. According to our estimates of energy properties, 1 Kg of P. juliflora could be used to substitute 0.5 L of diesel and furnace oil as well as 5 kWh (5 units) of electricity. As such, we recommend harvesting P. juliflora from BRW as a potential fuelwood energy source for local industries.
The Role of Aldh1a1 in Contributing to Breast Tumour Aggressiveness: a Study Conducted in an African Population
Authors: Gyan Eric , Green Andrew , Ahenkorah-Fondjo Linda , Jackson Andrew , Toss Micheal Shawky , Akakpo Patrick Kafui , Derkyi-Kwarteng Leonard , Rahman Ganiyu A. , Owiredu William K. B. A 
Published: August 2020

Abstract
Aldehyde dehydrogenase 1 member A1 (ALDH1A1) is one of the most well studied breast cancer stem cells. Its expression has been associated with poor clinicopathological features and clinical outcomes in several studies. This paper studies the expression of ALDH1A1 and its combination with CD44+/CD24−/low breast cancer stem cell and their association with clinicopathological parameters and molecular subtypes. METHOD: Tissue Microarray was constructed from 222 Formalin Fixed Paraffin Embedded (FFPE) breast cancer tissues. The expression of ALDH1A1, CD44 and CD24 were assessed by Immunohistochemistry (IHC). The association of ALDH1A1 and its association with clinicopathological parameters, molecular subtypes, CD44 and CD24 were studied in an African population. The association between CD44+/CD24−/low/ALDH1+ and the clinicopathological phenotypes were also studied. RESULTS: A high ALDH1A1 expression of 90% was recorded in this study. No association was found between ALDH1A1 and clinicopathological parameters. ALDH1A1 was positively associated with CD24 (r = 0.228, OR-4.599 95% CI- 1.751–12.076, p = 0.001) and CD44 (r = 0.228, OR-5.538 95%CI- 1.841–16.662, p = 0.001) but not associated with CD44+/CD24−/low (r = 0.134, OR- 2.720 95%CI- 0.959–7.710, p = 0.052). CD44+/CD24−/ALDH1+ however had significant associations with Age (p- 0.020, r = 0.161, OR- 2.771, 95%CI 1.147–6.697), Gender (p = 0.004, OR- 15.333 95%CI 1.339–175.54) ), Tumour grade(p = 0.005, r = 0.197, OR-3.913 95%CI 1.421–10.776) and clinical prognostic staging (p = 0.014, r = 0.182, OR-3.028 95%CI- 1.217–7.536). There was no association between CD44+/CD24−/ALDH1+ and the molecular subtypes. CONCLUSION: The high expression of ALDH1A1 in breast cancer makes it an important target for targeted therapy. This study further confirms the increased tumourigenicity of CD44+/CD24−/ALDH1+ combination phenotype and its association with increased tumour grade and clinical prognostic stage. Survival studies of ALDH1A1 and other breast cancer stem cells in African populations is strongly recommended to help further understand their effect on tumour aggressiveness.
Ultrasound Activated Nano TiO2 Loaded With Temozolomide Paves the Way for Resection of Chemo-resistant Glioblastoma Multiforme
Authors: Rehman Fawad Ur , Rauf Mohd Ahmar , Shaikh Sana , Qambrani Aqsa , Muhammad Pir , Hanif Sumaira 
Published: August 2020

Abstract
Background

Glioblastoma Multiforme (GBM) is one the most daunting issue to modern therapeutics, with a higher mortality rate post-diagnosis. Temozolomide (TMZ) is the only available treatment; however, the frequent resistance leaves the oncologists at the dead end. Therefore, new approaches to circumvent the GBM are highly desired. In this contribution, we have employed TiO2 nanosticks loaded with TMZ as nanomedicine for TMZ resistant GBM resection.
Results

The ultrasonication triple action effect could highly facilitate the tumor ablation by enhancing the TiO2 nanosticks traversing across BBB, releasing the TMZ payload from TiO2 nanosticks and Reactive Oxygen Species (ROS) generation from TiO2 nanosticks within GBM milieu. The tumor ablation was confirmed by MTT and Annexin(v)-PI assays, apoptotic proteins expression via western blot and ROS level detection in vitro, whereas tumor volume, weight, survival rate, and relative photon flux in the xenograft and orthoptic TMZ resistant GBM murine models as in vivo.
Conclusion

We found this nanomedicine-based ultrasound modality highly efficient in GBM treatment and is of future clinical application value due to employment of already FDA approved techniques and nanomedicine.
The complexity of scaling up an mHealth intervention. SMS for Life: A Case Study from a Health Systems Integration Perspective
Authors: Fruchtman Carmen Sant , Mbuyita Selemani , Mwanyika-Sando Mary , Braun Marcel , Savigny Don de , Cobos Daniel 
Published: August 2020

Abstract
Background The objective of this study was to apply systems thinking to examine the opportunities and challenges of scaling up and integrating a new electronic health technology into a complex health system. We used the implementation cycle of the supply chain management solution (SMS for Life 1.0) in Tanzania as a case study. SMS for Life was one of the earliest large-scale implementations of mHealth innovations worldwide. It aimed to bring visibility to antimalarial stock-outs through the use of SMS technology.Methods A qualitative case-study approach was used. This included a literature review, a document review of 61 project documents, a timeline of key events and the collection and analysis of 28 interviews with key stakeholders involved in or affected by the SMS for Life programme. Using the WHO mHealth Assessment and Planning for Scale (MAPS) Toolkit as a framework, we carried out a joint thematic analysis and identified the key reasons for the discontinuation of the programme.Results SMS for Life was reliable at scale and raised awareness of stock-outs with real-time monitoring. However, it was discontinued in 2015 after four years of a national rollout. The main reasons identified for the discontinuation were the programme’s failure to adapt to the continuous changes in Tanzania’s health system, the focus on stock-outs rather than ensuring appropriate stock management, and that it was perceived as costly by policy-makers.
GABAergic Neuron-Specific Whole-Brain Transduction by AAV-PHP.eB Incorporated with a New GAD65 Promoter
Authors: Hoshino Chiaki , Konno Ayumu , Kaneko Ryosuke , Hirai Hirokazu 
Published: August 2020

Abstract
GABAergic interneurons play a critical role in tuning neural networks in the central nervous system, and their defects cause neuropsychiatric disorders. Currently, the mDlx enhancer is solely used for adeno-associated virus (AAV) vector-mediated transgene delivery into cortical interneurons. Here, we developed a new inhibitory neuron-specific promoter of a 2.5-kb length from a genomic region of a mouse upstream of exon 1 of a gene encoding glutamic acid decarboxylase (GAD) 65 (mGAD65 promoter). Intravenous infusion of blood-brain barrier-penetrating AAV-PHPB expressing an enhanced green fluorescent protein under the control of the mGAD65 promoter transduced the whole brain in an inhibitory neuron-specific manner. The specificity and efficiency of the mGAD65 promoter for GABAergic interneurons, which was assessed at the motor cortex, were almost identical to or slightly higher than those of the mDlx enhancer. Immunohistochemical analysis revealed that the mGAD65 promoter preferentially transduced parvalbumin (PV)-expressing interneurons. Notably, the mGAD65 promoter transduced chandelier cells more efficiently than the mDlx enhancer and robustly labeled their synaptic boutons, called the cartridge, targeting the axon initial segments of excitatory pyramidal neurons. These results suggest that the mGAD65 promoter is useful for AAV-mediated targeting and the manipulation of GABAergic neurons with the dominance of cortical PV-expressing neurons, including chandelier cells.
Prevalence of Asymptomatic Visceral Leishmaniasis in Human and Dog, Benishangul Gumuz Regional State, Western Ethiopia
Authors: Bejano Shibabaw , Shumie Girma , Kumar Ashwani , Asemahagn Eyuel , Damte Demekech , Wolde Sinkinesh , Mulugeta Abate , Manaye Nigus , Bayih Abebe Genetu , Gadisa Endalamaw , Mamo Gezahegn 
Published: August 2020

Abstract
Background
Benishangul-Gumuz region is an important development corridor in Ethiopia. The large-scale projects; the great renaissance dam, mining and agriculture entailed huge environmental modification and settlement pattern changes. Detail epidemiological information of VL in the region is unknown.
Materials and Methods
A cross sectional study to assess the epidemiology and risk factors associated with asymptomatic VL. Leishmanin skin test (LST) was done for 1342 participants, and for 253 of them rK39 and DAT were done. Thirty-six dogs owned by households with LST positive member(s) were rK39 and DAT tested. A pretested questionnaire was used to capture individual and household characteristics.
Results
Of the 89.2% (1197/1342) who availed themselves for LST reading 6.0% were positive. The rk39 and DAT positivity among the 253 tested were 3.2% and 5.9% respectively. In dogs, positivity rates by rK39 and DAT were 13.9% and 5.6% respectively. Of the household and individual risk factors presence of dog in household (P = 0.005), male sex (0.003), residence woreda (0.000) and occupation (0.023) showed a strong positive association with LST positivity. Individuals who lived in household who owned dogs were 2.6 times more likely to be LST positive (AOR = 2.6; 95%CI = 1.54, 4.40). Being female decreased by 0.38 times the probability of being LST positive (AOR = 0.38; 95%CI = 0.20, 0.72). Living in Guba and Kurmuk has 4.7 (AOR = 4.74, 95% CI 1.83, 12.31) and 5.9 (AOR = 5.85, 95%CI 2.27, 15.09) times more risk of being infected.
Conclusions
We demonstrated presence of active VL transmission in the areas. Thus, we underline the need to establish the responsible vector(s) and reservoir(s) for comprehensive early containment plan to prevent potentially harmful public health and economic consequence.
Individualized Generation of Appropriate Assistance Timings for Active Lower Limb Robots via Machine Learning
Authors: Huang Guowei , Zhou Zikang , Liang Binghong , Nong Jinjin , Liu Youwei , Xie Longhan 
Published: August 2020

Abstract
Background: Obtaining appropriate assistance timings for individual users of active lower limb assistant robots (ALLARs) is one of the major challenges that limit the practical application of robots since very small assistance timing errors greatly affect the robot's assistance effect. However, neither theoretical nor experimental methods can currently generate appropriate assistance timings due to their respective availability or accuracy limitations. Method: In this paper, we proposed a new method to generate appropriate assistance timings for individual users of ALLARs via machine learning. The method has the accuracy of theoretical methods and the availability of experimental methods. We established a database of ten static physiological parameters, three dynamic parameters, and theoretical appropriate assistance timings, and mapped the static physiological parameters and the dynamic parameters to the theoretical assistance timings using multilayer neuron networks. Fold-cross validation and determination efficient were used to test the fit of the model. The root mean square error between generated values and true values of each subject was compared to that between the mean of the sample and the true values of each subject to evaluate the data accuracy of our method. We also set ±2% error as the boundary of the practical accuracy and compared the practical accuracy when using our method to that when using the mean generally. Result: The model achieved a small standard deviation of the square root error in the 10-fold cross-validation experiment and a large determination coefficient. We reduced the data error of starting and ending assistance timing from 0.0265 and 0.0172 to 0.014±0.000429 and 0.0079±0.000875, respectively, and improved the practical accuracy of starting and ending assistance timing from 54.93% and 75.49% to 89.54% and 99.95%, respectively.Conclusion: The proposed method can generate an appropriate assistance timings for different users of ALLARs walking at different speeds. Moreover, a new reference for ending assistance timings is provided and the database can be used as a reference for futer research. The practical effect of the method will be tested in future work.
Comparative Analysis of Postoperative Sexual Dysfunction and Quality of Life in Type A Aortic Dissection Patients of Different Ages
Authors: Luo Zeng-Rong , Chen Liang-Wan , Liao Dong-Shan 
Published: August 2020

Abstract
Background: To compare postoperative sexual dysfunction (SD) and quality of life (QOL) in Type A Aortic Dissection (AAD) Patients of Different Ages. Methods: From January 2018 to December 2019, 204 AAD postoperative survivors in Union Hospital of Fujian Medical University were selected and were divided into youth group (less than 50 years old) and elderly group (more than 50 years old). We evaluated SD according to the male International Erectile Dysfunction Index (IIEF-5) and female sexual function index (FSFI) . The Short Form 12 Health Survey Questionnaire (SF-12) and Quality of Life Enjoyment and Satisfaction Questionnaire (Q-LES-Q) were used to investigate the QOL, Quick Inventory Depressive Symptomatology-Self Report (QIDS-SR) and the Beck Depression Inventory-II (BDI-II) to investigate depressive symptoms. Results: 175 patients completed all the questionnaire (85.8%). The total SD prevalence rate was 38.9% (68 cases), with 27.4% of the youth (20 cases) and 47.1% of the elderly (48 cases). The age of non-SD and SD patients was 49.0±11.5 and 56.9±10.8 years, respectively (P=0.03). Compared with non-SD patients, the total physical health of SD patients was significantly worse (P=0.04), however, the mental health was not significantly worse (P=0.77); the depressive symptoms did not expressed a significant difference between the SD and non-SD groups (QIDS-SR P=0.15, BDI-Ⅱ P=0.06) . Total physical health in the youth SD group did not show significant better (P = 0.24), however, total mental health showed significantly worse (P=0.04), depressive symptoms scores were significantly higher (QIDS-SR P=0.03, BDI-Ⅱ P=0.04). Conclusion: The postoperative AAD SD prevalence of elderly is higher than that of youth, and the total physical health of SD patients is poorer than those without SD patients. The youth SD patients did not show a significant higher scores than the elderly SD patients, instead, the youth SD patients were more psychologically affected than the elderly SD patients, whose mental health was worse, and depression symptoms were more obvious, suggesting that the factors affecting the QOL of postoperative SD patients are related to physical factors, but the youth postoperative SD patients mainly affected by psychological factors.
A Genomic Blueprint of the Chicken Gut Microbiome
Authors: Gilroy Rachel , Ravi Anuradha , Getino Maria , Pursley Isabella , Horton Daniel , Alikhan Nabil-Fareed , Baker David , Gharbi Karim , Hall Neil , Watson Mick , Adriaenssens Evelien M. , Foster-Nyarko Ebenezer , Jarju Sheikh , Secka Arss , Antonio Martin , Oren Aharon , Chaudhuri Roy , Hildebrand Falk , Pallen Mark 
Published: August 2020

Abstract
Background: The chicken is the most abundant food animal in the world. However, despite its importance, the chicken gut microbiome remains largely undefined. Here, we exploit culture-independent and culture-dependent approaches to deliver a genomic blueprint of this complex microbial community. Results: We performed metagenomic sequencing of fifty chicken faecal samples from two breeds and analysed these, alongside all (n=582) relevant publicly available chicken metagenomes, to cluster over 20 million non-redundant genes and to construct over 5,500 metagenome-assembled bacterial genomes. In addition, we recovered nearly 600 bacteriophage genomes This represents the most comprehensive view of the chicken gut associated microbiome to date, encompassing dozens of novel candidate bacterial genera and hundreds of novel candidate species. Keen to provide a stable, clear and memorable nomenclature for novel species, we devised a scalable combinatorial system for the creation of hundreds of well-formed Latin binomials. We cultured bacterial isolates from faeces to deliver 282 whole genome sequences, incorporating thirty novel species, together with three species from the genus Escherichia, including the newly named species Escherichia whittamii.Conclusions: Our metagenomic and culture-based analyses provide new insights into the bacterial, archaeal and bacteriophage components of the chicken gut microbiome. The resulting datasets expand the known diversity of the chicken gut microbiome and provides a key resource for future high-resolution taxonomic and functional studies on the chicken gut microbiome.
Early Higher Achieved Mean Arterial Pressure Is Not Associated with Clinical Outcomes after Cardiac Arrest: A systematic Review and Meta-analysis
Authors: Wang Wen Jie , Feng Shun Yi , Zhang Su Li , Li Yong 
Published: August 2020

Abstract
Purpose: This study investigated the effect of early higher achieved mean arterial pressure (MAP) on clinical outcomes after cardiac arrest (CA).Methods: PubMed, ScienceDirect, Web of Science, China National Knowledge Infrastructure, and Wanfang databases were searched for relevant articles until May 2020. The meta-analysis produced pooled effect size represented by odds ratios (ORs) and corresponding 95% confidence intervals (CIs).Results: Nine studies that enrolled 1337 participants were considered eligible for this meta-analysis. Early higher achieved MAP was associated with similar mortality (OR 0.54, 95% CI 0.26 – 1.12; p = 0.10), neurologic outcome (OR 1.30, 95% CI 0.65 – 2.58; p = 0.46), length of ICU stay (OR -0.06, 95% CI -0.31 – 0.19; p = 0.46), mechanical ventilation time (OR -0.14, 95% CI -0.90 – 0.62; p = 0.72), and bleeding (OR 0.57, 95% CI 0.23 – 1.40; p = 0.22) compared with low MAP. Sensitivity analysis demonstrated that the result was solid. No publication bias across the studies was observed using Begg's and Egger’ funnel plot plot. Conclusion: Based on current evidence, this meta-analysis confirmed that early higher achieved MAP does not improve the clinical outcomes after CA.REGISTRATION: (PROSPERO: CRD42020189875)
Serum S100A8 as an early diagnostic biomarker in patients with community-acquired pneumonia
Authors: Zheng Ling , Fei Jun , Xu Zheng , Feng Chun-Mei , Li Se-Ruo , Fu Lin , Zhao Hui 
Published: August 2020

Abstract
Background and Objectives Limited studies suggested that calprotectin may take part in the pathophysiology of community-acquired pneumonia (CAP). Nevertheless, there is no clinical study to analyze the role of S100A8 in CAP patients. The objective of this study was to analyze the association of serum S100A8 with the severity of CAP based on a cross-sectional study. Methods Entire 200 CAP patients and 100 normal subjects were recruited. Demographic data, clinical information and serum were collected on admission. Serum S100A8 and inflammatory cytokines were detected. Results Serum S100A8 was increased in CAP patients on admission. Serum S100A8 was gradually increased in parallel with the CAP severity scores. Serum S100A8 was positively correlated with CAP severity scores (CURB-65, CRB-65, PSI, CURXO and SMART-COP), blood routine parameters (WBC, neutrophil-lymphocyte ratio and monocyte-lymphocyte ratio) and inflammatory cytokines (TNFα, IL-1β and CRP). Furtherly, univariate and multivariate logistical regression analysis revealed that there was a positive association between serum S100A8 with CRB-65, PSI and CURXO. Moreover, the predictive capacity of serum S100A8 was performed by receiver operating characteristic area under the curve (AUC) analysis. The AUCs of S100A8 for CAP and CAP severity were 0.855 and 0.893, respectively. Mechanistic analysis found that S100A8 knockdown alleviated streptococcus pneumoniae-evoked inflammatory cytokines in A549 cells. Conclusion Serum S100A8 on admission was positively associated with the severity of CAP. S100A8 knockdown alleviates streptococcus pneumoniae- evoked inflammatory cytokines in A549 cells, indicating that S100A8 may exert an important role in the pathophysiology of CAP and be an early serum diagnostic biomarker for CAP.