bluuebunny/crossref_metadata_2025_split huggingface dataset:
The effects of Rhodopseudomonas palustris PSB06 and CGA009 with different agricultural applications on rice growth and rhizosphere bacterial communities
Authors: Luo Luyun , Wang Pei , Zhai Zhongying , Su Pin , Tan Xinqiu , Zhang Deyong , Zhang Zhuo , Liu Yong 
Published: October 2019

Abstract
In recent years, the photosynthetic bacteria have been used widely in agriculture, but the effects of different agricultural applications on crop rhizosphere microorganism and crops are lack. In this study, we provide new insights into the structure and composition of the rice root-associated microbiomes as well as the effect on crop of the Rhodopseudomonas palustris(R. palustris) PSB06 and CGA009 at the rice seedling stage with seed immersion and root irrigation. Compare with CK group, the length of stem, the peroxidase (POD), and superoxide dismutase (SOD) activities in PSB06 treatment group was significantly higher, while the length of stem in CGA009 treatment group was significantly higher. The POD and SOD activities in CGA009 treatment groups only were higher slightly than the CK group. In the study, the dominant phyla were Proteobacteria (51.95–61.66%), Bacteroidetes (5.40–9.39%), Acidobacteria (4.50–10.52%), Actinobacteria (5.06–8.14%), Planctomycetes (2.90–4.48%), Chloroflexi (2.23–5.06%) and Firmicutes (2.38–7.30%), accounted for 87% bacterial sequences. The principal coordinate analysis (pCoA) and mantel results showed the two application actions of R. palustris CGA009 and PSB06 had significant effects on rice rhizosphere bacterial communities (p < 0.05). The PSB06 can significantly promote the rice growth and enhance stress resistance of rice at the seedling stage, while the R. palustris CGA009 has no significant effect on rice. Dissimilarity test and canonical correspondence analysis (CCA) results showed that the TN and pH were the key factors affecting rice rhizosphere bacterial community in the seedling stage. This study will provide some guidance advices for the study of the microecological regulation of photosynthetic bacteria on crops.
XszenFHal, a novel tryptophan 5-halogenase from Xenorhabdus szentirmaii
Authors: Domergue Jérémy , Erdmann Diane , Fossey-Jouenne Aurélie , Petit Jean-Louis , Debard Adrien , de Berardinis Véronique , Vergne-Vaxelaire Carine , Zaparucha Anne 
Published: October 2019

Abstract
Flavin-dependent halogenases (FHals) catalyse the halogenation of electron-rich substrates, mainly aromatics. Halogenated compounds have many applications, as pharmaceutical, agrochemicals or as starting materials for the synthesis of complex molecules. By exploring the sequenced bacterial diversity, we discovered and characterized XszenFHal, a novel FHal from Xenorhabdus szentirmaii, a symbiotic bacterium of entomopathogenic nematode. The substrate scope of XszenFHal was examined and revealed activities towards tryptophan, indole and indole derivatives, leading to the formation of the corresponding 5-chloro products. XszenFHal makes a valuable addition to the panel of flavin-dependent halogenases already discovered and enriches the potential for biotechnology applications by allowing access to 5-halogenated indole derivatives.
Large volume infusions of hydroxyethyl starch during cardiothoracic surgery may be associated with postoperative kidney injury: propensity-matched analysis
Authors: Matsunaga Wataru , Sanui Masamitsu , Sasabuchi Yusuke , Kobayashi Yasuma , Kitajima Asuka , Yanase Fumitaka , Takisawa Yutaka , Lefor Alan Kawarai 
Published: October 2019

Abstract

Background
The safety of intraoperative administration of hydroxyethyl starch (HES) has been debated. We hypothesized that intraoperative use of HES is associated with postoperative acute kidney injury (AKI) following cardiopulmonary bypass (CPB).

Materials and methods
Patients who underwent cardiothoracic surgery using CPB between 2007 and 2014 were retrospectively reviewed. The incidence of AKI within 7 days after surgery, defined by the Kidney Disease Improving Global Outcome criteria, was compared for patients who did or did not receive 6% (70/0.5) or 6% (130/0.4) HES for anesthesia management before or after CPB. Multivariable logistic regression and propensity matching analysis were performed to examine whether use of HES is associated with postoperative AKI. Outcomes comparing patients receiving HES ≥ 1000 mL and < 1000 mL were also compared.

Results
Data from 1976 patients were reviewed. All patients received 70/0.5 HES as a part of the priming solution for CPB. The incidence of postoperative AKI was 28.2% in patients who received HES and 26.0% in patients who did not (p = 0.33). In multivariable analysis, there was no correlation between the use of HES and the incidence of AKI (odds ratio 0.87, 95% CI 0.30–2.58, p = 0.81). Propensity matching showed that the incidence of AKI was not significantly different between 481 patients administered with HES and 962 patients (26.6% vs. 26.9%, p = 0.95) who did not receive HES for anesthesia management. However, peak creatinine levels, needed for renal replacement therapy, and in-hospital mortality were higher, and 28-day hospital-free days were lower in patients receiving HES ≥ 1000 mL than those receiving HES < 1000 mL (p < 0.05).

Conclusions
Intraoperative use of HES was not associated with postoperative AKI following CPB. However, administration of large volumes of HES may be associated with kidney-related adverse clinical outcomes.
Gemtuzumab ozogamicin and novel antibody-drug conjugates in clinical trials for acute myeloid leukemia
Authors: Yu Bo , Liu Delong 
Published: October 2019

Abstract
Targeted agents are increasingly used for the therapy of acute myeloid leukemia (AML). Gemtuzumab ozogamicin (GO) is the first antibody-drug conjugate (ADC) approved for induction therapy of AML. When used in fractionated doses, GO combined with the conventional cytarabine/anthracycline-based induction chemotherapy significantly improves the outcome of previously untreated AML patients. Single-agent GO is effective and safe for AML patient ineligible for intensive chemotherapy. Multiple combination regimens incorporating GO have also been recommended as potential alternative options. In addition, several novel ADCs targeting CD33, CD123 and CLL-1 are currently undergoing preclinical or early clinical investigations. In this review, we summarized the efficacy and limitations of GO as well as novel ADCs for adult AML patients.
Avian influenza virus surveillance in migratory birds in Egypt revealed a novel reassortant H6N2 subtype
Authors: Zanaty Ali M. , Erfan Ahmed M. , Mady Wessam H. , Amer Fatma , Nour Ahmed A. , Rabie Neveen , Samy Mohamed , Selim Abdullah A. , Hassan Wafaa M. M. , Naguib Mahmoud M. 
Published: October 2019

AbstractBackgroundAvian influenza viruses (AIVs) have been identified from more than 100 different species of wild birds around the globe. Wild migratory birds can act as potential spreaders for AIVs to domestic birds between different countries. Egypt is situated on important migratory flyways for wild birds between different continents. While much is known about circulation of zoonotic potential H5N1 and H9N2 AIVs in domestic poultry in Egypt, little is known about the pivotal role of migratory birds in the maintenance and transmission of the viruses in Egypt.MethodsTargeted AIV surveillance has been conducted in 2017 in different wetlands areas in Northern and Eastern Egypt.ResultsAIV of subtype H5 was detected in two bird species. In addition, a novel reassortant strain of the H6N2 subtype was identified which reveals the continuous risk of new influenza virus(es) introduction into Egypt. This novel virus possesses a reassortant pattern originating from different AIV gene pools.ConclusionsIntervention control strategies should be performed to minimize the possible contact of domestic birds with wild birds to lower the risk of virus transmission at this interface. In addition, constant monitoring of AIVs in migratory birds is essential in the early detection of influenza virus introduction into Egypt.
Transdermal delivery of Minoxidil using HA-PLGA nanoparticles for the treatment in alopecia
Authors: Jeong Woo Yeup , Kim Sodam , Lee So Yun , Lee Hyeseon , Han Dong Wook , Yang Seung Yun , Kim Ki Su 
Published: October 2019

Abstract

Background
Alopecia has become a very common disease that many people around the world are suffered. Minoxidil (MXD) is the most well-known commercialized drug in its treatment. However, in the case of MXD administration, there are some problems with low efficiency of transdermal delivery and additional side effects.

Method
MXD and Rhodamine B (Rho B) are encapsulated in poly(Lactide-co-Glycolide) grafted hyaluronate nanoparticles (HA-PLGA/MXD NPs, HA-PLGA/Rho B NPs) which is prepared with W/O/W solvent evaporation method. After then, the investigation is carried out to confirm the feasibility of NPs in alopecia treatment.

Results
Both of HA-PLGA/MXD NPs and HA-PLGA/Rho B NPs are successfully prepared. In addition, it is confirmed that HA-PLGA NPs sufficiently delivered to cells without any significant cytotoxicity by cell viability, cellular uptake and skin permeation test.

Conclusion
Taken together, HA-PLGA NPs as a transdermal delivery carrier to hair follicle cells can be exploited to develop the efficient and effective platform of transdermal drug delivery for the treatment of various diseases.
Quantitative and structural analysis of isotopically labelled natural crosslinks in type I skin collagen using LC-HRMS and SANS
Authors: Zhang Yi , Naffa Rafea , Garvey Christopher J. , Maidment Catherine A. , Prabakar Sujay 
Published: October 2019

Abstract
Collagen structure in biological tissues imparts its intrinsic physical properties by the formation of several covalent crosslinks. For the first time, two major crosslinks in the skin dihydroxylysinonorleucine (HLNL) and histidinohydroxymerodesmosine (HHMD), were isotopically labelled and then analysed by liquid-chromatography high-resolution accurate-mass mass spectrometry (LC-HRMS) and small-angle neutron scattering (SANS). The isotopic labelling followed by LC-HRMS confirmed the presence of one imino group in both HLNL and HHMD, making them more susceptible to degrade at low pH. The structural changes in collagen due to extreme changes in the pH and chrome tanning were highlighted by the SANS contrast variation between isotopic labelled and unlabelled crosslinks. This provided a better understanding of the interaction of natural crosslinks with the chromium sulphate in collagen suggesting that the development of a benign crosslinking method can help retain the intrinsic physical properties of the leather. This analytical method can also be applied to study artificial crosslinking in other collagenous tissues for biomedical applications.

Graphical abstract
LyoPRONTO: an Open-Source Lyophilization Process Optimization Tool
Authors: Shivkumar Gayathri , Kazarin Petr S. , Strongrich Andrew D. , Alexeenko Alina A. 
Published: October 2019

Abstract
This work presents a new user-friendly lyophilization simulation and process optimization tool, freely available under the name LyoPRONTO. This tool comprises freezing and primary drying calculators, a design-space generator, and a primary drying optimizer. The freezing calculator performs 0D lumped capacitance modeling to predict the product temperature variation with time which shows reasonably good agreement with experimental measurements. The primary drying calculator performs 1D heat and mass transfer analysis in a vial and predicts the drying time with an average deviation of 3% from experiments. The calculator is also extended to generate a design space over a range of chamber pressures and shelf temperatures to predict the most optimal setpoints for operation. This optimal setpoint varies with time due to the continuously varying product resistance and is taken into account by the optimizer which provides varying chamber pressure and shelf temperature profiles as a function of time to minimize the primary drying time and thereby, the operational cost. The optimization results in 62% faster primary drying for 5% mannitol and 50% faster primary drying for 5% sucrose solutions when compared with typical cycle conditions. This optimization paves the way for the design of the next generation of lyophilizers which when coupled with accurate sensor networks and control systems can result in self-driving freeze dryers.
Voiding profile in recipients post renal transplant: A prospective observational study
Authors: Parikesit Dyandra , Wicaksono Indra , Tawfid Muhamad Iqbal , Widia Fina , Rahardjo Harrina Erlianti 
Published: October 2019

Background: Renal transplantation (RTX) is thought to have high survival rates. However, patients with long-term dialysis have decreased bladder function due to disuse. High urine production after RTX surgery might cause patients to have urinary symptoms, thus decreasing their quality of life. The aim of this study was to evaluate voiding characteristics of patients after RTX surgery. Methods: All patients were diagnosed with chronic kidney disease (CKD) and underwent kidney transplantation from a living donor. Anthropometric parameters, physical examinations, cause of CKD, daily urine production, types and duration of dialysis, and basic laboratory examination were collected before transplant surgery. Post-operative examinations included laboratory examination, international prostate symptom score (IPSS; for male patients only), overactive bladder symptom score (OABSS), uroflowmetry, and post void residue (PVR). Results: 71 patients were evaluated with a mean age of 46 ± 17.9 years, with male and female ratio of 52:19. Significant negative correlation was seen between duration of dialysis and daily urine production (r: -0.68, p<0.01). Majority of patients had a maximum flow rate of >15 cc/s (70.4 %) with average flow of 22 ± 9.8 cc/s. The majority of patients had PVR <100 cc (91.5%) with median PVR of 33.5 cc (range, 2.3 – 142 cc). IPSS result showed that frequency [2 (0 – 5)] and nocturia [2 (0 – 5)] are the main problem in these patients (n = 52). OABSS questionnaire also showed that frequency (OABSS 1; score 1 [1-2]) and nocturia (OABSS 2; score 2 [0-3]) was the main symptoms reported by patients. Conclusion: After RTX, the majority of patients experienced urinary frequency and nocturia problems due to various causes such as increased daytime and nighttime urine production, urinary tract infection, changes in bladder capacity (both small and large), and a decrease in bladder compliance.
Recent Developments in Tuberculous Meningitis Pathogenesis and Diagnostics
Authors: Cresswell Fiona V , Davis Angharad G. , Sharma Kusum , Basu Roy Robindra , Ganiem Ahmad Rizal , Kagimu Enock , Solomons Regan , Wilkinson Robert J. , Bahr Nathan C , Thuong Nguyen Thuy Thuong ,   Tuberculous Meningitis International Research Consortium
Published: October 2019

The pathogenesis of Tuberculous meningitis (TBM) is poorly understood, but contemporary molecular biology technologies have allowed for recent improvements in our understanding of TBM. For instance, neutrophils appear to play a significant role in the immunopathogenesis of TBM, and either a paucity or an excess of inflammation can be detrimental in TBM. Further, severity of HIV-associated immunosuppression is an important determinant of inflammatory response; patients with the advanced immunosuppression (CD4+ T-cell count of <150 cells/μL) having higher CSF neutrophils, greater CSF cytokine concentrations and higher mortality than those with CD4+ T-cell counts > 150 cells/μL. Host genetics may also influence outcomes with LT4AH genotype predicting inflammatory phenotype, steroid responsiveness and survival in Vietnamese adults with TBM. Whist in Indonesia, CSF tryptophan level was a predictor of survival, suggesting tryptophan metabolism may be important in TBM pathogenesis. These varying responses mean that we must consider whether a “one-size-fits-all” approach to anti-bacillary or immunomodulatory treatment in TBM is truly the best way forward. Of course, to allow for proper treatment, early and rapid diagnosis of TBM must occur. Diagnosis has always been a challenge but the field of TB diagnosis is evolving, with sensitivities of at least 70% now possible in less than two hours with GeneXpert MTB/Rif Ultra. In addition, advanced molecular techniques such as CRISPR-MTB and metagenomic next generation sequencing may hold promise for TBM diagnosis. Host-based biomarkers and signatures are being further evaluated in childhood and adult TBM as adjunctive biomarkers as even with improved molecular assays, cases are still missed. A better grasp of host and pathogen behaviour may lead to improved diagnostics, targeted immunotherapy, and possibly biomarker-based, patient-specific treatment regimens.
Checklists to guide the supportive and critical care of tuberculous meningitis
Authors: Donovan Joseph , Rohlwink Ursula K. , Tucker Elizabeth W. , Hiep Nguyen Thi Thu , Thwaites Guy E. , Figaji Anthony A. ,   Tuberculous Meningitis International Research Consortium
Published: October 2019

The assessment and management of tuberculous meningitis (TBM) is often complex, yet no standardised approach exists, and evidence for the clinical care of patients, including those with critical illness, is limited. The roles of proformas and checklists are increasing in medicine; proformas provide a framework for a thorough approach to patient care, whereas checklists offer a priority-based approach that may be applied to deteriorating patients in time-critical situations. We aimed to develop a comprehensive assessment proforma and an accompanying ‘priorities’ checklist for patients with TBM, with the overriding goal being to improve patient outcomes. The proforma outlines what should be asked, checked, or tested at initial evaluation and daily inpatient review to assist supportive clinical care for patients, with an adapted list for patients in critical care. It is accompanied by a supporting document describing why these points are relevant to TBM. Our priorities checklist offers a useful and easy reminder of important issues to review during a time-critical period of acute patient deterioration. The benefit of these documents to patient outcomes would require investigation; however, we hope they will promote standardisation of patient assessment and care, particularly of critically unwell individuals, in whom morbidity and mortality remains unacceptably high.
Pamięć i trauma
Authors: Wasiak Katarzyna 
Published: October 2019

Memory and Trauma: Contemporary Interpretations of the 1992‑1995 War among the Youth of Bosnian‑Muslim
For Bosnia and Herzegovina, the 1990s were a period of changes due to war. Transformations occurred not only in the political area, but also in the social one. A multicultural region, Bosnia and Herzegovina was suddenly transformed into isolated enclaves. In fact, this separation is maintained by war trauma, which remains in the social consciousness and regulates ethnic relations in the state.
A estratégia de informação de Marcello Caetano o último governante do Estado Novo
Authors: Cabrera Ana 
Published: October 2019

Este artigo analisa a estratégia de informação levada a cabo por Marcello Caetano quando, em setembro de 1968, substituiu Salazar na presidência do Conselho de Ministros. Esta estratégia consistiu na realização de acontecimentos que lhe assegurassem uma boa visibilidade na imprensa, afirmação de uma relação mais próxima com os jornalistas, e realização das conversas em famíliateledifundidas.
A investigação assenta no estudo do perfil político de Marcello Caetano suportada por fontes (cartas, textos, livros e entrevistas) e na analise de jornais da época (A Capital, Diário de Lisboa, Diário Popular, Diário de Notícias) no período compreendido entre setembro de 1968 e setembro de 1969. Como conclusão assinala-se que a construção da imagem deste político na imprensa favoreceu o inicio da sua governação através da construção de uma personalidade política, social e familiar bem distinta da de Salazar.
Environmental Licensing Vs. Equitable and Sustainable Development: The Incidence of the Principle of Proportionality as a Weighing and Choice Instrument in the Brazilian Judicial System
Authors: Farias Athena De Albuquerque , Campelo Maria Paula Silvestre , Brito Francisca Ergovânia Batista  de 
Published: October 2019

Abstract: The increase in the chances of potential environmental catastrophes due to excessive anthropic activity has caused society to turn its efforts to protect the environment. The notion of Sustainable Development was raised aiming economic growth based on the rational use of existing natural resources, for the purpose of preserving them for future generations. The concept of Equitable and Sustainable Development was created, which encompasses several aspects of society besides economic and environmental, with the main purpose of stimulate socially equitable development together with a sustainable environment. Environmental licensing consists of an administrative instrument used by the State to regulate potentially harmful activities to the environment. In this, it is sometimes necessary to consider constitutional principles, which requires verification of which should prevail, depending on the conflicting case. The present study defends the effectiveness of the use of the Principle of Proportionality in the act of weighing constitutionally protected interests, where the particularities of the concrete case must be analyzed in order to verify which institute should overlap, seeking the harmonization between free enterprise and the right to a balanced environment, these being the main principles involved in environmental licensing. Keywords: Environmental Law. Administrative law. Sustainable Development. Environmental license. Principle of proportionality.
Mucosal Injuries by Tegumentary Leishmaniasis: A Systematic Review
Authors: Feitosa Pedro Walisson Gomes , Oliveira Italo Constancio de , Moura Rayane Da Silva , Grangeiro Yasmin De Alencar , Rodrigues Elisa Hellen Cruz , Caracas Heloísa Fernandes , Pinheiro Sally De França Lacerda 
Published: October 2019

Abstract: American Tegumentary Leishmaniasis (ATL) it’s the name given to the amount of infection diseases that cause immunological and polymorphic changes on the skin and mucosa. Caused by the contamination of protozoa of the genus Leishmania, the pathogenesis in question can manifest itself in the Tegumentary (mucosa and cutaneous) and in the visceral form, depending on the parasite and the host. The aim of this study was to collect the scientific knowledge produced about the Mucosa Tegumentary Leishmaniasis, presenting the epidemiological, clinical, diagnostic and therapeutic relations available in the researched literature. It was applied the Muños Protocol (2002) in published articles between 1981 and 2018 and indexed in the database of the Health Virtual Library (HVL) that was selected for this review study. The research strategy used was: “Tegumentary Leishmaniasis” and “Mucosa”, including, too, the following limits: articles in Portuguese, English and Spanish, available. The works present interfaces of Mucosa Tegumentary Leishmaniasis, stating epidemiological, clinical, diagnostic and therapeutic data of the pathology. It is evidenced the importance of the realization of studies about this theme to the development of new medical treatments. It is noticed that there is still a limited number of researches in this field, becoming necessary new studies about the theme. Keywords: Mucosal injuries. Leishmaniasis. Infection diseases.