bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparison of the Effect of Insecticides on Bumble Bees (Bombus impatiens) and Mosquitoes (Aedes aegypti and Culex quinquefasciatus) by Standard Mosquito Research Methods
Authors: McGregor Bethany L , Giordano Bryan V , Runkel Alfred E , Nigg Herbert N , Nigg H Lee , Burkett-Cadena Nathan D 
Published: November 2020

Abstract
Mosquito control districts in the United States are limited to two main classes of adulticides, pyrethroids and organophosphates, to control mosquitoes. Two adulticides used to control domestic mosquitoes are Fyfanon EW (malathion, organophosphate) and DeltaGard (deltamethrin, pyrethroid). While the effect of these pesticides on European honeybees (Apis mellifera L., Hymenoptera: Apidae) has been investigated, effects on native pollinators need additional research. The purpose of this study was to investigate the acute nontarget effects of these pesticides on Bombus impatiens Cresson (Hymenoptera: Apidae), a native North American bumble bee species, and compare these effects to wild and laboratory strains of mosquitoes (Aedes aegypti (L.) and Culex quinquefasciatus Say, Diptera: Culicidae) through field and laboratory assays. Bombus impatiens was found to be resistant to Fyfanon EW (x̅ = 6.7% mortality at 50-µg malathion per bottle) at levels that caused significant mortality to study mosquitoes (86.2 ≥ x̅ ≥ 100% mortality) in laboratory bottle bioassays. Comparatively, B. impatiens demonstrated greater mortality to DeltaGard (93.3%) at 2.5-µg deltamethrin/bottle than any mosquito colony assayed (14.1 ≥ x̅ ≥ 87.0% mortality). Only DeltaGard was tested in field applications. In the field, we observed acute effects of DeltaGard on mosquitoes and B. impatiens at 25- and 75-m distance from a truck-mounted ultra-low volume fogger, although treatment effects were not significant for B. impatiens. Additional wild-caught nontarget mortality to DeltaGard field trials was also evaluated. This study indicated that common mosquito control adulticides do cause nontarget mortality to B. impatiens but that impacts are variable depending on pesticide and further studies are needed.
Evaluation of Sticky Traps for Monitoring Scolothrips sexmaculatus (Thysanoptera: Thripidae) and Stethorus punctum (Coleoptera: Coccinellidae) as Predators of Spider Mites in California Almonds
Authors: Haviland David R , Rill Stephanie M , Gordon Chelsea A 
Published: November 2020

Abstract
Changes in almond production practices have caused sixspotted thrips, Scolothrips sexmaculatus Pergande, and the coccinellid beetle, Stethorus punctum LeConte, to replace phytoseiid mites as the dominant predators of spider mites in California almonds. We conducted a series of field studies to evaluate nine commercially available adhesive traps for S. sexmaculatus and S. punctum and found that the yellow strip trap was the most effective, least expensive, and easy to use. At peak population levels, individual cards routinely caught &gt;1,000 S. sexmaculatus and &gt;100 S. punctum trap−1 week−1. We documented that larger traps collected more S. sexmaculatus, and more S. sexmaculatus per square area, suggesting that the trap surface was attractive. We determined the number of traps needed to have 50, 70, and 90% confidence that the averages of trap captures were within 10, 30, and 50% of the population mean. Two yellow strip traps per orchard provided 90% confidence that trap captures were within 50% of the population mean if there were an average of 16.0 S. sexmaculatus trap−1 week−1 or 7.9 S. punctum trap−1 week−1. Populations required to attain the same levels of confidence using four traps per orchard were 3.9 S. sexmaculatus and 2.9 S. punctum trap−1 week−1. We conclude that use of the yellow strip trap to monitor for natural enemies, in combination with leaf samples for spider mites, has the potential to improve integrated pest management programs for spider mites, and assist future research to understand the biology and phenology of both predator species.
Frame-validity Games and Lower Bounds on the Complexity of Modal Axioms
Authors: Balbiani Philippe , Fernández-Duque David , Herzig Andreas , Iliev Petar 
Published: November 2020

Abstract
We introduce frame-equivalence games tailored for reasoning about the size, modal depth, number of occurrences of symbols and number of different propositional variables of modal formulae defining a given frame property. Using these games, we prove lower bounds on the above measures for a number of well-known modal axioms; what is more, for some of the axioms, we show that they are optimal among the formulae defining the respective class of frames.
MxMPK6-2-bHLH104 interaction is involved in reactive oxygen species signaling in response to iron deficiency in apple rootstock
Authors: Li Duyue , Sun Qiran , Zhang Guifen , Zhai Longmei , Li Keting , Feng Yi , Wu Ting , Zhang Xinzhong , Xu Xuefeng , Wang Yi , Han Zhenhai 
Published: November 2020

Abstract
Iron (Fe) is a trace element necessary for plant growth. Many land plants have evolved a set of mechanisms associated with the Fe absorption process to deal with the problem of insufficient Fe supply in the soil. During Fe absorption, reactive oxygen species (ROS) can be used as a signal to initiate a response to stress caused by Fe deficiency. However, the molecular mechanisms underlying the involvement of ROS in the Fe deficiency stress response remains unclear. In this study, we have identified a kinase, MxMPK6-2, from Malus xiaojinensis, an apple rootstock that is highly efficient at Fe absorption. MxMPK6-2 has been shown to be responsive to ROS signals during Fe deficiency, and MxMPK6-2 overexpression in apple calli enhanced its tolerance to Fe deficiency. We further screened for proteins in the Fe absorption pathway and identified MxbHLH104, a transcription factor which interacts with MxMPK6-2. MxbHLH104 can be phosphorylated by MxMPK6-2 in vivo, and we confirmed that its phosphorylation increased Fe absorption in apple calli under Fe deficiency, with the presence of ROS promoting this process. Overall, we have demonstrated that MxMPK6-2 is responsive to ROS signaling during Fe deficiency, and is able to control its response by regulating MxbHLH104.
The nature of the modification at position 37 of tRNAPhe correlates with acquired taxol resistance
Authors: Pan Yu , Yan Tong-Meng , Wang Jing-Rong , Jiang Zhi-Hong 
Published: November 2020

Abstract
Acquired drug resistance is a major obstacle in cancer therapy. Recent studies revealed that reprogramming of tRNA modifications modulates cancer survival in response to chemotherapy. However, dynamic changes in tRNA modification were not elucidated. In this study, comparative analysis of the human cancer cell lines and their taxol resistant strains based on tRNA mapping was performed by using UHPLC–MS/MS. It was observed for the first time in all three cell lines that 4-demethylwyosine (imG-14) substitutes for hydroxywybutosine (OHyW) due to tRNA-wybutosine synthesizing enzyme-2 (TYW2) downregulation and becomes the predominant modification at the 37th position of tRNAphe in the taxol-resistant strains. Further analysis indicated that the increase in imG-14 levels is caused by downregulation of TYW2. The time courses of the increase in imG-14 and downregulation of TYW2 are consistent with each other as well as consistent with the time course of the development of taxol-resistance. Knockdown of TYW2 in HeLa cells caused both an accumulation of imG-14 and reduction in taxol potency. Taken together, low expression of TYW2 enzyme promotes the cancer survival and resistance to taxol therapy, implying a novel mechanism for taxol resistance. Reduction of imG-14 deposition offers an underlying rationale to overcome taxol resistance in cancer chemotherapy.
Cell Therapy for Stroke: A Mechanistic Analysis
Authors: Gu Ben Jiahe , Kung David K , Chen Han-Chiao Isaac 
Published: November 2020

Abstract
Cell therapy has been widely recognized as a promising strategy to enhance recovery in stroke survivors. However, despite an abundance of encouraging preclinical data, successful clinical translation remains elusive. As the field continues to advance, it is important to reexamine prior clinical trials in the context of their intended mechanisms, as this can inform future preclinical and translational efforts. In the present work, we review the major clinical trials of cell therapy for stroke and highlight a mechanistic shift between the earliest studies, which aimed to replace dead and damaged neurons, and later ones that focused on exploiting the various neuromodulatory effects afforded by stem cells. We discuss why both mechanisms are worth pursuing and emphasize the means through which cell replacement can still be achieved.
SARS-CoV-2 Serologic Assay Needs for the Next Phase of the US COVID-19 Pandemic Response
Authors: Gundlapalli Adi V , Salerno Reynolds M , Brooks John T , Averhoff Francisco , Petersen Lyle R , McDonald L Clifford , Iademarco Michael F ,   CDC COVID-19 Response, Carroll Yulia I , Gorwitz Rachel , Lin-Gibson Sheng , Wang Lili , Pinto Ligia A 
Published: November 2020

Abstract

Background
There is a need for validated and standardized severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) quantitative immunoglobulin G (IgG) and neutralization assays that can be used to understand the immunology and pathogenesis of SARS-CoV-2 infection and support the coronavirus disease 2019 (COVID-19) pandemic response.

Methods
Literature searches were conducted to identify English language publications from peer-reviewed journals and preprints from January 2020 through November 6, 2020. Relevant publications were reviewed for mention of IgG or neutralization assays for SARS-CoV-2, or both, and the methods of reporting assay results.

Results
Quantitative SARS-CoV-2 IgG results have been reported from a limited number of studies; most studies used in-house laboratory-developed tests in limited settings, and only two semiquantitative tests have received US Food and Drug Administration (FDA) Emergency Use Authorization (EUA). As of November 6, 2020, there is only one SARS-CoV-2 neutralization assay with FDA EUA. Relatively few studies have attempted correlation of quantitative IgG titers with neutralization results to estimate surrogates of protection. The number of individuals tested is small compared with the magnitude of the pandemic, and persons tested are not representative of disproportionately affected populations. Methods of reporting quantitative results are not standardized to enable comparisons and meta-analyses.

Conclusions
Lack of standardized SARS-CoV-2 quantitative IgG and neutralization assays precludes comparison of results from published studies. Interassay and interlaboratory validation and standardization of assays will support efforts to better understand antibody kinetics and longevity of humoral immune responses postillness, surrogates of immune protection, and vaccine immunogenicity and efficacy. Public–private partnerships could facilitate realization of these advances in the United States and worldwide.
Learning about the Neighborhood
Authors: Gao Zhenyu , Sockin Michael , Xiong Wei 
Published: November 2020

AbstractWe develop a model to analyze information aggregation and learning in housing markets. Households enter a neighborhood by buying houses and consuming each other’s final goods. In the presence of pervasive informational frictions, housing prices serve as important signals to households and capital producers about the neighborhood’s economic strength. Our model provides a novel amplification mechanism in which noise from housing markets propagates throughout the local economy via learning because of the complementarity in households’ decisions, distorting migration into the neighborhood and the supply of capital and labor. We provide consistent evidence based on the recent U.S. housing cycle.
In Uteroand Lactational Exposure to an Environmentally Relevant Mixture of Brominated Flame Retardants Induces a Premature Development of the Mammary Glands
Authors: Gouesse Rita-Josiane , Dianati Elham , McDermott Alec , Wade Michael G , Hales Barbara , Robaire Bernard , Plante Isabelle 
Published: November 2020

AbstractIn utero and prepubertal development of the mammary glands occurs minimally in a hormone independent manner until puberty where maturation of the hypothalamic-pituitary-gonadal axis drives an extensive remodeling. Nevertheless, because the immature glands contain functional hormone receptors, they are especially vulnerable to the effects of endocrine disruptors, such as brominated flame retardants (BFRs). BFRs are widespread chemicals added to household objects to reduce their flammability, and to which humans are ubiquitously exposed. We previously reported that in utero and lactational exposure to BFRs resulted in an impaired mammary gland development in peripubertal animals. Here, we assessed whether BFR-induced disruption of mammary gland development could manifest earlier in life. Dams were exposed prior to mating until pups’ weaning to a BFR mixture (0, 0.06, 20, or 60 mg/kg/day) formulated according to levels found in house dust. The mammary glands of female offspring were collected at weaning. Histo-morphological analyses showed that exposure to 0.06 mg/kg/day accelerates global epithelial development as demonstrated by a significant increase in total epithelial surface area, associated with a tendency to increase of the ductal area and thickness, and of lumen area. Significant increases of the Ki67 cell proliferation index and of the early apoptotic marker cleaved caspase-9 were also observed, as well as an upward trend in the number of thyroid hormone receptor α1 positive cells. These molecular, histologic, and morphometric changes are suggestive of accelerated pubertal development. Thus, our results suggest that exposure to an environmentally relevant mixture of BFRs induces precocious development of the mammary gland.
An update on the microbiome in vasculitis
Authors: Tariq Shahna , Clifford Alison H. 
Published: November 2020

Purpose of review
To summarize recent evidence regarding the presence and potential role of the microbiome in systemic vasculitides.

Recent findings
Microbiomic descriptions are now available in patients with small, medium and large vessel vasculitis. The majority of studies have evaluated gastrointestinal inhabitants, with a smaller number of studies describing the nasal, pulmonary or vascular microbiomes. Most published studies are observational and cross-sectional. Dysbiosis is seen frequently in vasculitis patients with reduced microbial diversity observed in nasal, fecal and vascular samples compared with disease and/or healthy controls. Predominant bacteria vary, but overall, patients with vasculitis tend to have more pathogenic and less commensal bacteria in active disease. In the few longitudinal studies available, improvement or resolution of dysbiosis has been observed following vasculitis treatment and improved disease activity.

Summary
Dysbiosis and reduced microbial diversity has been identified in patients with small, medium and large vessel vasculitis. Although limited data suggests microbiomes may ‘normalize’ following immunosuppression, cause or effect cannot be determined. It is hypothesized that microbial disruption in a genetically susceptible individual may trigger excessive host immune activation and vasculitis; however, larger studies with longitudinal and translational design are needed to further our current understanding.
Role of adipose tissues in osteoarthritis
Authors: Zapata-Linares Natalia , Eymard Florent , Berenbaum Francis , Houard Xavier 
Published: November 2020

Purpose of review
Epidemiologic studies reveal that the link between obesity and osteoarthritis cannot be uniquely explained by overweight-associated mechanical overload. For this reason, much attention focuses on the endocrine activity of adipose tissues. In addition to the systemic role of visceral and subcutaneous adipose tissues, many arguments highlight the involvement of local adipose tissues in osteoarthritis.

Recent findings
Alteration in MRI signal intensity of the infrapatellar fat pad may predict both accelerated knee osteoarthritis and joint replacement. In this context, recent studies show that mesenchymal stromal cells could play a pivotal role in the pathological remodelling of intra-articular adipose tissues (IAATs) in osteoarthritis. In parallel, recent findings underline bone marrow adipose tissue as a major player in the control of the bone microenvironment, suggesting its possible role in osteoarthritis.

Summary
The recent description of adipose tissues of various phenotypes within an osteoarthritic joint allows us to evoke their direct involvement in the initiation and progression of the osteoarthritic process. We can expect in the near future the discovery of novel molecules targeting these tissues.
Cerebral vasculitis associated with drug abuse
Authors: Younger David S. 
Published: November 2020

Purpose of review
To review understand the epidemiology, background, neuropharmacology, and histopathology of literature verified cases, and likely etiopathogenic mechanisms.

Recent findings
There are only a handful of histologically confirmed patients in the literature with cerebral vasculitis because of drug abuse.

Summary
There is little justification for invasive laboratory investigation given the ready availability of highly accurate vascular neuroimaging techniques to dictate management, which usually rests upon avoidance of further exposure and minimizing the secondary neurotoxic effects of the abused substances and polypharmacy use.
Effects of Axial Compression and Distraction on Vascular Bud and VEGFA Expression in the Vertebral Endplate of an Ex Vivo Rabbit Spinal Motion Segment Culture Model
Authors: Zhan Jia-Wen , Wang Shang-Quan , Feng Min-Shan , Gao Jing-Hua , Wei Xu , Yu Jie , Yin Xun-Lu , Yin He , Sun Kai , Chen Ming , Xie Rui , Zhang Ping , Zhu Li-Guo 
Published: November 2020

Study Design.
An ex vivo study of the rabbit's vertebral endplate.

Objective.
The aim of this study was to assess the effect of axial compression and distraction on vascular buds and vascular endothelial growth factor (VEGFA) expression of the vertebral endplate (VEP).

Summary of Background Data.
The abnormal load can lead to intervertebral disc degeneration (IDD), whereas axial distraction can delay this process. The effects of different mechanical loads on the intervertebral disc (IVD) have been hypothesized to be related to changes in the vascular buds of the VEP; moreover, the process that might involve the vascular endothelial growth factor (VEGF) within the VEP.

Methods.
Rabbit spinal segments (n = 40) were harvested and randomly classified into four groups: Control group, no stress was applied; Group A, a constant compressive load applied; Group B, compression load removed for a fixed time daily on a continuous basis, and substituted with a distraction load for 30 minutes; and Group C, compression removed for 30 minutes for a fixed period daily on a continuous basis. Tissue specimens were collected before the culture (day 0) and on day 14 post-culture of each group for analysis of IVDs’ morphology, and protein and mRNA expression of Aggrecan, COL2al, VEGFA, and vascular endothelial growth factor receptor 2 of the VEPs.

Results.
Application of axial distraction and dynamic load compression significantly delayed time- and constant compression-mediated VEP changes and IDD. Moreover, the degree of degeneration was associated with loss of vascular buds, as well as the downregulation of VEGFA and its receptor.

Conclusion.
The regulation of vascular buds and VEGF expression in the VEP represents one of the mechanisms of axial distraction and dynamic loading.

Level of Evidence: N/A
Telemedicine in the Evaluation and Management of Neurosurgical Spine Patients
Authors: Greven Alexander C.M. , McGinley Beau M. , Guisse Ndeye F. , McGee Lynessa J. , Pirkle Sarah , Malcolm James G. , Rodts Gerald E. , Refai Daniel , Gary Matthew F. 
Published: November 2020

Study Design.
Retrospective questionnaire study of all patients seen via telemedicine during the COVID-19 pandemic at a large academic institution.

Objective.
This aim of this study was to compare patient satisfaction of telemedicine clinic to in-person visits; to evaluate the preference for telemedicine to in-person visits; to assess patients’ willingness to proceed with major surgery and/or a minor procedure based on a telemedicine visit alone.

Summary of Background Data.
One study showed promising utility of mobile health applications for spine patients. No studies have investigated telemedicine in the evaluation and management of spine patients.

Methods.
An 11-part questionnaire was developed to assess the attitudes toward telemedicine for all patients seen within a 7-week period during the COVID-19 crisis. Patients were called by phone to participate in the survey. χ2 and the Wilcoxon Rank-Sum Test were performed to determine significance.

Results.
Ninety-five percent were “satisfied” or “very satisfied” with their telemedicine visit, with 62% stating it was “the same” or “better” than previous in-person appointments. Patients saved a median of 105 minutes by using telemedicine compared to in-person visits. Fifty-two percent of patients have to take off work for in-person visits, compared to 7% for telemedicine. Thirty-seven percent preferred telemedicine to in-person visits. Patients who preferred telemedicine had significantly longer patient-reported in-person visit times (score mean of 171) compared to patients who preferred in-person visits (score mean of 137, P = 0.0007). Thirty-seven percent of patients would proceed with surgery and 73% would proceed with a minor procedure based on a telemedicine visit alone.

Conclusion.
Telemedicine can increase access to specialty care for patients with prolonged travel time to in-person visits and decrease the socioeconomic burden for both patients and hospital systems. The high satisfaction with telemedicine and willingness to proceed with surgery suggest that remote visits may be useful for both routine management and initial surgical evaluation for spine surgery candidates.

Level of Evidence: 3