bluuebunny/crossref_metadata_2025_split huggingface dataset:
ABCC4/MRP4 contributes to the aggressiveness of Myc‐associated epithelial ovarian cancer
Authors: Jung Moonsun , Gao Jixuan , Cheung Leanna , Bongers Angelika , Somers Klaartje , Clifton Molly , Ramsay Emma E. , Russell Amanda J. , Valli Emanuele , Gifford Andrew J. , George Joshy , Kennedy Catherine J. , Wakefield Matthew J. , Topp Monique , Ho Gwo‐Yaw ,   Australian Ovarian Cancer Study, Scott Clare L. , Bowtell David D. , deFazio Anna , Norris Murray D. , Haber Michelle , Henderson Michelle J. 
Published: April 2020

AbstractEpithelial ovarian cancer (EOC) is a complex disease comprising discrete histological and molecular subtypes, for which survival rates remain unacceptably low. Tailored approaches for this deadly heterogeneous disease are urgently needed. Efflux pumps belonging to the ATP‐binding cassette (ABC) family of transporters are known for roles in both drug resistance and cancer biology and are also highly targetable. Here we have investigated the association of ABCC4/MRP4 expression to clinical outcome and its biological function in endometrioid and serous tumors, common histological subtypes of EOC. We found high expression of ABCC4/MRP4, previously shown to be directly regulated by c‐Myc/N‐Myc, was associated with poor prognosis in endometrioid EOC (P = .001) as well as in a subset of serous EOC with a “high‐MYCN” profile (C5/proliferative; P = .019). Transient siRNA‐mediated suppression of MRP4 in EOC cells led to reduced growth, migration and invasion, with the effects being most pronounced in endometrioid and C5‐like serous cells compared to non‐C5 serous EOC cells. Sustained knockdown of MRP4 also sensitized endometrioid cells to MRP4 substrate drugs. Furthermore, suppression of MRP4 decreased the growth of patient‐derived EOC cells in vivo. Together, our findings provide the first evidence that MRP4 plays an important role in the biology of Myc‐associated ovarian tumors and highlight this transporter as a potential therapeutic target for EOC.
CEO horizon problem and characteristics of board of directors and compensation committee
Authors: Liu Ruonan , Liu Zhenfeng 
Published: April 2020

AbstractExtant research finds inconclusive evidence about the CEO horizon problem. One possible explanation is that board of directors, especially compensation committees, intervene to mitigate the CEO horizon problem. In this study, we examine whether the characteristics of board of directors and compensation committee affect their effectiveness in mitigating the CEO horizon problem. We find that retiring CEOs are more likely to reduce R&D expenditures when CEOs have more power, and director tenure is longer; retiring CEOs in firms with large board of directors and compensation committee are less likely to manage accruals.
Hsa_circ_0031288/hsa‐miR‐139‐3p/Bcl‐6 regulatory feedback circuit influences the invasion and migration of cervical cancer HeLa cells
Authors: Xu Ya Jie , Yu He , Liu  Guang Xin 
Published: April 2020

AbstractCircular RNA (circRNA) molecules contain microRNA (miRNA) response elements that are able to competitively bind miRNAs as well as function as miRNA sponges within cells, which can reduce miRNA inhibition of target genes, thereby increasing their expression. TargetScan and miRanda bioinformatic tools were used to analyze the binding sites between genes. The relative levels of gene expression in tissues and cells were verified using quantitative reverse transcription‐polymerase chain reaction. Inhibition of cell proliferation was detected using a WST‐8 method. Cell invasion ability and migration ability were assessed using a Transwell migration assay and a scratch assay, respectively. The binding of miRNA and circRNA was detected using an RNA pull‐down assay. Bifluorescence reporter gene vectors were constructed to verify the binding of miRNA to messenger RNA. A tumor model of cervical cancer cell transplantation in mice was constructed to observe the effect of the genes on tumor growth. hsa_circ_0031288 and B‐cell CLL/lymphoma 6 (Bcl‐6) exhibited high expression in cervical cancer cells and tissue, while hsa‐miR‐139‐3p exhibited low expression. Reducing hsa_circ_0031288 and Bcl‐6 expression or increasing hsa‐miR‐139‐3p expression significantly inhibited the migration, invasion, proliferation, and growth of xenograft and HeLa cells. hsa_circ_0031288 had a regulatory effect on hsa‐miR‐139‐3p, and hsa‐miR‐139‐3p targeted the 3′ untranslated region of Bcl‐6. Reducing hsa_circ_0031288 expression promoted hsa‐miR‐139‐3p expression, while overexpressing miR‐139‐3p inhibited the transcription of Bcl‐6. In the cervical cancer HeLa cell line, the hsa_circ_0031288/hsa‐miR‐139‐3p/Bcl‐6 regulatory axis affects cell migration and proliferation, and its mechanism may involve hsa_circ_0031288 acting as a sponge for hsa‐miR‐139‐3p, thereby relieving the transcriptional inhibition of Bcl‐6. This suggests an approach for elucidating the pathogenesis of cervical cancer while offering new intervention targets for cervical cancer treatment.
Long noncoding RNA SOX21‐AS1 regulates the progression of triple‐negative breast cancer through regulation of miR‐520a‐5p/ORMDL3 axis
Authors: Liu Xia , Song Jingyong , Kang Yu , Wang Yaojia , Chen Anyue 
Published: April 2020

AbstractRecently, long noncoding RNAs (lncRNAs) have been reported as a new kind of controllers about cancer processes in biology. In spite of the dysregulation of lncRNAs in various kinds of cancers, only a little of the information was effective on the expression configuration and inner effects of lncRNAs in triple‐negative breast cancer (TNBC). This study valued the expression of lncRNA SOX21‐AS1 and the biological role it played in TNBC. In our research, SOX21‐AS1 had a high expression in TNBC cell lines. The functional experiments showed that knockdown of SOX21‐AS1 obviously restrained cell proliferation, migration, invasion, and epithelial‐mesenchymal transition process and promoted cell apoptosis. Mechanistically, SOX21‐AS1 was found to bind with miR‐520a‐5p. Besides, ORMDL3 was identified as a downstream target of miR‐520a‐5p, and the suppressed ORMDL3 expression induced by silenced SOX21‐AS1 could be restored by miR‐520a‐5p inhibition. Further, data from rescue assays revealed that SOX21‐AS1 could regulate the malignancy of TNBC via miR‐520a‐5p/ORMDL3 axis. All in all, we identified that SOX21‐AS1 regulated the cellular process of TNBC cells via antagonizing miR‐520a‐5p availability to upregulate ORMDL3 expression.
LncRNA MALAT1 targeting miR‐124‐3p regulates DAPK1 expression contributes to cell apoptosis in Parkinson's Disease
Authors: Lu Yi , Gong Zhongying , Jin Xiaojie , Zhao Peng , Zhang Yuting , Wang Zhiyun 
Published: April 2020

AbstractDeath associated protein kinase 1 (DAPK1) was initially discovered in the progress of gamma‐interferon induced programmed cell death, it is a key factor in the central nervous system, including Parkinson's disease (PD). However, the underlying mechanisms of DAPK1 in PD remain unclear and this research work aims to explore the potential mechanisms of DAPK1 in PD. In the study, we exposed SH‐SY5Y cells to MPP+ and treated mice with MPTP to investigate the roles of DAPK1 in PD and the underlying mechanisms. The results indicated that the expression of DAPK1 is significantly upregulated and negatively correlated with miR‐124‐3p levels in SH‐SY5Y cells treated by MPP+, and miR‐124‐3p mimics could effectively inhibit DAPK1 expressions and alleviate MPP+‐induced cell apoptosis. In addition, knockdown MALAT1 reduces the levels of DAPK1 and the ratio of SH‐SY5Y cell apoptosis, which is reversed via miR‐124‐3p inhibitor in vitro. Similarly, knockdown MALAT1 could improve behavioral changes and reduce apoptosis by miR‐124‐3p upregulation and DAPK1 downregulation in MPTP induced PD mice. Taken together, our data showed that lncRNA MALAT1 positively regulates DAPK1 expression by targeting miR‐124‐3p, and mediates cell apoptosis and motor disorders in PD. In summary, these results suggest that MALAT1/miR‐124‐3p /DAPK1 signaling cascade mediates cell apoptosis in vitro and in vivo, which may provide experimental evidence of developing potential therapeutic strategies for PD.
Exosomes derived from RPE cells under oxidative stress mediate inflammation and apoptosis of normal RPE cells through Apaf1/caspase‐9 axis
Authors: Ke Yifeng , Fan Xiaoe , Rui Hao , Xinjun Ren , Dejia Wen , Chuanzhen Zheng , Li Xiaorong 
Published: April 2020

AbstractThis study aims to explore the effects of exosomes, secreted by retinal pigment epithelial (RPE) cells under oxidative stress (OS), on apoptosis and inflammation of normal RPE cells. Exosomes secreted by normal RPE cells (named as exo) and rotenone (2.5 µmol/L) stimulated RPE cells (named as rot‐exo) were isolated and extracted by multi‐step differential centrifugation for morphology observation under a transmission electron microscopy. pcDNA3.1a, pcDNA3.1a‐Apaf1, and p3xFlag‐CMV‐caspase‐9 plasmids were constructed and transfected into ARPE‐19 cells. Exosomes secreted by ARPE‐19 cells were injected into the vitreous body of rats to verify the effect of Apaf1 and caspase‐9 on cell apoptosis and inflammation. Co‐immunoprecipitation was applied to clarify the interaction of Apaf1 with caspase‐9. Exosomes secreted by rotenone stimulated ARPE‐19 cells could induce cell apoptosis, oxidative injury, and inflammation in ARPE‐19 cells. Exosomes secreted under OS can damage retinal functions of rats and have upregulated expression of Apaf1. Overexpression of Apaf1 in exosomes secreted under OS can cause the inhibition of cell proliferation, the increase of cell apoptosis and elicitation of inflammatory response in ARPE‐19 cells. Exosomes derived from ARPE‐19 cells under OS regulate Apaf1 expression to increase cell apoptosis and to induce oxidative injury and inflammatory response through a caspase‐9 apoptotic pathway.
Deep cerebral venous blood flow monitoring by transcranial doppler ultrasonography in the treatment of cerebral venous sinus thrombosis: A case report
Authors: Zhang Hui , He Yitao , Zou Liangyu , Deng Jian , Guo Yi 
Published: April 2020

AbstractWe report the case of a patient in whom we used deep cerebral venous blood flow monitoring by transcranial Doppler ultrasonography to monitor the effect of anticoagulation therapy on cerebral venous sinus thrombosis. The blood flow velocity of deep cerebral veins increased in the early stage of cerebral venous sinus thrombosis, then gradually decreased to the normal level as the disease improved. Moreover, the recovery of the blood flow velocity of deep cerebral veins occurred earlier than the morphological recovery demonstrated by magnetic resonance venography.
Evaluating the impact of turf‐care products on soil biological health
Authors: Diera Alexx A. , Raymer Paul L. , Martinez‐Espinoza Alfredo D. , Bauske Ellen , Habteselassie Mussie Y. 
Published: April 2020

AbstractGolf courses require extensive use of inputs to meet the needs of playability and aesthetics. The impact of these inputs on soil biological health is largely unknown. Two field trials were conducted at a golf course in Georgia to evaluate short‐term effects of wetting agents (Cascade Plus and Duplex [C+D], Revolution [Rev]), plant growth regulators (PrimoMaxx [PM] and Cutless [CL]), and a product called PlantHelper (PH) on soil biological health by measuring microbial abundance and function. Quantitative polymerase chain reaction was used to measure microbial abundance, which included total bacteria, total fungi, and ammonia‐oxidizing prokaryotes. Soil respiration and enzyme assays were used as additional indicators of soil health. In bentgrass putting green, total bacteria and ammonia‐oxidizing bacteria decreased in abundance in response to the wetting agents and PH, indicating their sensitivity to the products. Whereas C+D stimulated urease activity, Rev and PH caused a short‐lived but immediate increase in respiration, indicating that they acted as labile carbon sources. In a bermudagrass fairway, PM was the only product that caused an increase in total bacteria abundance. PrimoMaxx and CL caused a delayed increase in respiration, suggesting that they may have affected the microorganisms indirectly through their impact on root growth and exudate production later. Although CL caused a decrease in urease activity, none of the products significantly affected phosphatase activity. Overall, the products did not seem to have a lasting impact on soil biological health, although long‐term studies are needed to confirm these observations.
Understanding access to genomics in an ethnically diverse south Florida population: A comparison of demographics in odyssey and rapid whole genome sequencing programs
Authors: Hussain Saida B. , Quittner Alexandra L. , Brown Marilyn , Li‐Rosi Ana Maria 
Published: April 2020

AbstractAdvances in genomic medicine have evolved to include rapid whole genome sequencing (rWGS) in pediatric intensive care settings. Traditionally, genetic testing was conducted in outpatient clinics, with stepwise genetic testing occurring over several years. This delayed the time to diagnosis, making it more difficult to include underrepresented groups, such as those who identify as Black and Latinx. National genetic sequencing programs have also struggled to engage these participants in their studies, leading to a significant disparity in access to new genetic technologies. The purpose of our study was to compare the demographic characteristics of families enrolled in both an Odyssey Program (N = 46), defined as outpatients in the Genetics Clinic who have had prior genetic testing, and a newly implemented rWGS (N = 52) sequencing program. Despite living in a large, ethnically diverse city, our results indicated that parents in the Odyssey program differed significantly from parents in the rWGS program in level of education, family income, and insurance status. For example, 71.5% of parents in the diagnostic Odyssey program had a college or advanced degree, whereas 42% of parents in the rWGS program had this level of education. Family income and insurance also differed, with 48.6% of families in the Odyssey program earning $100,000 or more versus 28.2% in rWGS; 56% of parents in the Odyssey program had private insurance with 26% on Medicaid whereas only 23% of parents in rWGS had private insurance, with the vast majority of children on Medicaid (69%). Thus, our Odyssey program illustrates some of the common pitfalls in implementing genomic testing in an ethnically diverse community, including lack of referrals, travel to outpatient visits, and a cultural mismatch with providers. The successful enrollment of underrepresented groups in the rWGS program demonstrates that given the opportunity to participate in genetic testing, families are interested and aware of the potential benefits of this testing for their child. As genomic sequencing transitions from outpatient to inpatient settings, an opportunity arises to close the health disparity gap. Recommendations for implementing rWGS in pediatric, intensive care settings that address the common barriers faced by underrepresented families are discussed.
Idiopathic mesenteric phlebosclerosis: A rare cause of chronic diarrhea
Authors: Sze Shun Fung , Lam Polly Woon Yee , Lam Jodis Ting Wa , Chung Cliff Chi Chiu , Lee Yuk Tong 
Published: April 2020

AbstractWe report a 45‐year‐old healthy Chinese woman who presented with chronic diarrhea and iron deficiency anemia, with colonoscopy showing multiple ulcers from cecum to sigmoid on a background of dark‐purple mucosa. She was initially suspected to be suffering from inflammatory bowel disease, but the peculiar colonic biopsy findings and computed tomography (CT) imaging features, together with her habit of using Chinese herbal supplements, supported the rare diagnosis of idiopathic mesenteric phlebosclerosis.
Switching protease inhibitors to rilpivirine in HIV‐positive individuals with complete viral suppression and without prior HIV drug resistance in a resource‐limited setting: a randomized controlled trial
Authors: Palanuphap Kulissara , Sungkanuparph Somnuek 
Published: April 2020

AbstractIntroductionPrior to the availability of rilpivirine (RPV), patients who could not tolerate efavirenz and nevirapine (NVP) were treated with protease inhibitor (PI)‐based antiretroviral therapy (ART). Dyslipidaemia and other metabolic complications are commonly associated with PI use. This study aimed to compare the efficacy and adverse events between switching from PI‐based to RPV‐based regimen, versus continuing PI‐based regimens in HIV‐positive individuals with complete viral suppression.MethodsA randomized controlled trial was conducted in HIV‐positive individuals receiving PI‐based regimens with undetectable HIV RNA and without prior HIV drug resistance. Patients were enrolled between July and December 2017 in a university medical centre in Bangkok, Thailand. They were randomized to switch from PIs to RPV (switch group) or continue ritonavir‐boosted PI (control group). Primary endpoint was the proportion of patients with undetectable HIV RNA at 48 weeks. Changes in CD4 cell counts, lipid profiles and adverse events were also analysed.Results and discussionA total of 84 patients were enrolled, 42 in each group. Mean age was 47.7 years and 53.6% were males. At 48 weeks, 95.2% of patients in the switch group and 92.9% of control group had maintained undetectable HIV RNA (difference rate 2.4%; 95% CI, −9.6 to 14.7). Means of CD4 cell counts were 611 and 641 cells/mm3 in switch and control groups respectively (p = 0.632). Mean changes in lipid profiles (switch vs. control groups) were: total cholesterol, −12.5 versus + 12.2 (p = 0.024); LDL, −3.4 versus + 6.2 (p = 0.040); HDL, +1.6 versus + 1.9 (p = 0.887); and triglycerides, −82.6 versus − 24.4 mg/dL (p = 0.031). The mean changes of glucose and eGFR were similar (p > 0.05) between the two groups. The mean change of ALT was significantly greater in switch group (18.2 vs. 4.0 U/L, p = 0.017). One patient in switch group had anorexia and elevated ALT at 14 weeks and completely recovered after RPV discontinuation.ConclusionsSwitching PIs to RPV, in patients with complete viral suppression and without prior HIV drug resistance, sustains viral suppression and yields better lipid profiles. This finding supports its use as switching therapy in patients receiving PI‐based regimens due to intolerance to efavirenz and NVP and previous alternatives limited to PI in resource‐limited settings.
Preparation of polydopamine‐coated, graphene oxide/Fe3O4‐ imprinted nanoparticles for selective removal of sulfonylurea herbicides in cereals
Authors: He Jinxing , Lu Yue , Zhao Tao , Li Yingqiu 
Published: April 2020

AbstractBACKGROUNDSulfonylureas are potentially toxic broad‐spectrum herbicides. They pose a persistent threat to food safety and the environment. It is therefore important to develop a rapid and efficient pretreatment and detection method to prevent their harmful effects on human health.RESULTSIn the present work, a novel and highly selective absorbent for chlorosulfuron (CS) detection was prepared by the simple self‐polymerization of dopamine on the surface of magnetic graphene oxide using a CS template. The resultant imprinted nanoparticles (MGO@PDA‐MIPs) were characterized by transmission electron microscopy, X‐ray diffraction, vibrating‐sample magnetometry, thermogravimetric analysis, and nitrogen adsorption–desorption. The adsorption experiments demonstrated that the MGO@PDA‐MIPs have excellent selectivity with regard to CS, with a high imprinting factor of 3.41 compared with a non‐imprinted polymer. The nanoparticles rapidly achieve adsorption equilibrium and efficient desorption because there are numerous binding sites on the thin polydopamine imprinting layer. Under optimized conditions, the MGO@PDA‐MIPs can be used to detect sulfonylurea residues in cereal samples by magnetic solid phase extraction coupled with high performance liquid chromatography (HPLC). The nanoparticles have a satisfactory recovery rate (80.65–101.01%) with a relative standard deviation (RSD) of less than 7.15%, and a limit of detection with regard to CS of 1.61 μg kg−1 (S/N = 3). They can also be re‐used at least seven times.CONCLUSIONThe MGO@PDA‐MIPs have outstanding recognition performance, and can be prepared by a facile, single‐step, and environmentally friendly process. They therefore have excellent potential for the recognition and separation of trace sulfonylurea herbicides in complex matrices. © 2020 Society of Chemical Industry
Chemical profiling and marker characterization of Huangqin decoction prepared with three types of peony root by liquid chromatography with electrospray ionization mass spectrometry
Authors: Du Kang , Yang Jingyi , Yang Li , Wang Zhengtao , Wang Rui , Shi Yanhong 
Published: April 2020

AbstractClarification of the quality and biological effect equivalence of traditional Chinese medicines containing multi‐origin species is essential to improve their current quality standards, and also is the core problem to clarify the origins of single herbs with multi‐species in Chinese formulas that will guarantee their clinical application. Huangqin decoction is the typical one of multi‐origin formulas frequently used in traditional Chinese medicine and Kampo medicine. An ultra high performance liquid chromatography with electrospray ionization–tandem mass spectrometry was developed for chemical profiling and marker quantification of Huangqin decoction prepared with two different original types of peony root, white and red peony root. Forty‐seven main peaks in chemical profiling of Huangqin decoction prepared with white and red peony root were identified: nine were from peony root, 20 from baical skullcap root, 17 from licorice root, and one from jujubae fruit. The markers characteristics of the respective types of peony root in Huangqin decoction differ from that in single herbs, especially in terms of monoterpenoids and hydrolysable tannins. Subsequently, 17 representative markers in Huangqin decoction prepared with three types of peony root and their chemical characteristics and content distribution were carried out.
Validation of Abbreviated Four‐ and Eight‐Item Versions of the PTSD Checklist for DSM‐5 in a Traumatically Injured Sample
Authors: Geier Timothy J. , Hunt Joshua C. , Hanson Jessica L. , Heyrman Katelyn , Larsen Sadie E. , Brasel Karen J. , deRoon‐Cassini Terri A. 
Published: April 2020

AbstractRates of posttraumatic stress disorder (PTSD) are three times higher in traumatically injured populations than the general population, yet limited brief, valid measures for assessing PTSD symptom severity exist. The PTSD Checklist for DSM‐5 (PCL‐5) is a valid, efficient measure of symptom severity, but its completion is time consuming. Subsequently, abbreviated four‐ and eight‐item versions were developed using the Mini‐International Neuropsychiatric Interview–7 PTSD module and validated in Veteran samples. This study aimed to validate these abbreviated versions using the Clinician‐Administered PTSD Scale for DSM‐5 (CAPS‐5), the gold standard for PTSD diagnosis, in a traumatically injured civilian population. Participants were 251 traumatically injured adults (Mage = 42.52 years; 69.3% male; 50.2% Caucasian) recruited from a Level 1 trauma center inpatient unit; 32.3% and 17.9% of participants experienced a motor vehicle crash or gunshot wound, respectively. The CAPS‐5 and PCL‐5 were administered approximately 6.5 months postinjury. We examined whether compared to the full PCL‐5, the abbreviated versions would adequately differentiate between participants with and without a CAPS‐5 PTSD diagnosis. The abbreviated versions were highly correlated with the total scale and showed good‐to‐excellent internal consistency. The diagnostic utility of the abbreviated measures was comparable to that of the total scale regarding sensitivity, suggesting they may be useful as abbreviated screening tools; however, the total scale functioned better regarding specificity. The abbreviated versions of the PCL‐5 may be useful screening instruments in the long‐term care of traumatic injury survivors and may be more likely to be implemented across routine clinical and research contexts.