bluuebunny/crossref_metadata_2025_split huggingface dataset:
Anti-Aβ antibodies bound to neuritic plaques enhance microglia activity and mitigate tau pathology
Authors: Laversenne Vanessa , Nazeeruddin Sameer , Källstig Emma C. , Colin Philippe , Voize Christel , Schneider Bernard L. 
Published: November 2020

AbstractThe brain pathology of Alzheimer’s disease (AD) is characterized by the misfolding and aggregation of both the amyloid beta (Aβ) peptide and hyperphosphorylated forms of the tau protein. Initial Aβ deposition is considered to trigger a sequence of deleterious events contributing to tau pathology, neuroinflammation and ultimately causing the loss of synapses and neurons. To assess the effect of anti-Aβ immunization in this context, we generated a mouse model by overexpressing the human tau protein in the hippocampus of 5xFAD mice. Aβ plaque deposition combined with human tau overexpression leads to an array of pathological manifestations including the formation of tau-positive dystrophic neurites and accumulation of hyperphosphorylated tau at the level of neuritic plaques. Remarkably, the presence of human tau reduces microglial clustering in proximity to the Aβ plaques, which may affect the barrier role of microglia. In this mouse model, continuous administration of anti-Aβ antibodies enhances the clustering of microglial cells even in the presence of tau. Anti-Aβ immunization increases plaque compaction, reduces the spread of tau in the hippocampal formation and prevents the formation of tau-positive dystrophic neurites. However, the treatment does not significantly reduce tau-induced neurodegeneration in the dentate gyrus. These results highlight that anti-Aβ immunization is able to enhance microglial activity around neuritic plaques, mitigating part of the tau-induced pathological manifestations.
TERT promoter mutation confers favorable prognosis regardless of 1p/19q status in adult diffuse gliomas with IDH1/2 mutations
Authors: Arita Hideyuki , Matsushita Yuko , Machida Ryunosuke , Yamasaki Kai , Hata Nobuhiro , Ohno Makoto , Yamaguchi Shigeru , Sasayama Takashi , Tanaka Shota , Higuchi Fumi , Iuchi Toshihiko , Saito Kuniaki , Kanamori Masayuki , Matsuda Ken-ichiro , Miyake Yohei , Tamura Kaoru , Tamai Sho , Nakamura Taishi , Uda Takehiro , Okita Yoshiko , Fukai Junya , Sakamoto Daisuke , Hattori Yasuhiko , Pareira Eriel Sandika , Hatae Ryusuke , Ishi Yukitomo , Miyakita Yasuji , Tanaka Kazuhiro , Takayanagi Shunsaku , Otani Ryohei , Sakaida Tsukasa , Kobayashi Keiichi , Saito Ryuta , Kurozumi Kazuhiko , Shofuda Tomoko , Nonaka Masahiro , Suzuki Hiroyoshi , Shibuya Makoto , Komori Takashi , Sasaki Hikaru , Mizoguchi Masahiro , Kishima Haruhiko , Nakada Mitsutoshi , Sonoda Yukihiko , Tominaga Teiji , Nagane Motoo , Nishikawa Ryo , Kanemura Yonehiro , Kuchiba Aya , Narita Yoshitaka , Ichimura Koichi 
Published: November 2020

AbstractTERT promoter mutations are commonly associated with 1p/19q codeletion in IDH-mutated gliomas. However, whether these mutations have an impact on patient survival independent of 1p/19q codeletion is unknown. In this study, we investigated the impact of TERT promoter mutations on survival in IDH-mutated glioma cases. Detailed clinical information and molecular status data were collected for a cohort of 560 adult patients with IDH-mutated gliomas. Among these patients, 279 had both TERT promoter mutation and 1p/19q codeletion, while 30 had either TERT promoter mutation (n = 24) or 1p/19q codeletion (n = 6) alone. A univariable Cox proportional hazard analysis for survival using clinical and genetic factors indicated that a Karnofsky performance status score (KPS) of 90 or 100, WHO grade II or III, TERT promoter mutation, 1p/19q codeletion, radiation therapy, and extent of resection (90–100%) were associated with favorable prognosis (p < 0.05). A multivariable Cox regression model revealed that TERT promoter mutation had a significantly favorable prognostic impact (hazard ratio = 0.421, p = 0.049), while 1p/19q codeletion did not have a significant impact (hazard ratio = 0.648, p = 0.349). Analyses incorporating patient clinical and genetic information were further conducted to identify subgroups showing the favorable prognostic impact of TERT promoter mutation. Among the grade II-III glioma patients with a KPS score of 90 or 100, those with IDH-TERT co-mutation and intact 1p/19q (n = 17) showed significantly longer survival than those with IDH mutation, wild-type TERT, and intact 1p/19q (n = 185) (5-year overall survival, 94% and 77%, respectively; p = 0.032). Our results demonstrate that TERT promoter mutation predicts favorable prognosis independent of 1p/19q codeletion in IDH-mutated gliomas. Combined with its adverse effect on survival among IDH-wild glioma cases, the bivalent prognostic impact of TERT promoter mutation may help further refine the molecular diagnosis and prognostication of diffuse gliomas.
Does substrate colour affect the visual appearance of gilded medieval sculptures? Part I: colorimetry and interferometric microscopy of gilded models
Authors: Wu Qing , Hauldenschild Meret , Rösner Benedikt , Lombardo Tiziana , Schmidt-Ott Katharina , Watts Benjamin , Nolting Frithjof , Ganz David 
Published: November 2020

Abstract
In the history of medieval gilding, a common view has been circulated for centuries that the substrate colour can influence the visual appearance of a gilded surface. In order to fully understand the correlation between the gilding substrate and the colour appearance of the gold leaf laid above, in this paper (Part I) analytical techniques such as colorimetry and interferometric microscopy are implemented on models made from modern gold leaves. This study demonstrates that the substrate colour is not perceptible for gold leaf of at least 100 nm thickness, however the surface burnishing can greatly alter the visual appearance of a gold surface, and the quality of the burnishing is dependent on the substrate materials. Additionally, surface roughness and texture of the substrate can play supplementary roles, which can be visually observed through digital microscopy and quantified through interferometric microscopy. The findings in this paper will form the basis for the study of gold leaf samples taken from medieval European gilded sculptures in Part II.
A survey and analysis of intrusion detection models based on CSE-CIC-IDS2018 Big Data
Authors: Leevy Joffrey L. , Khoshgoftaar Taghi M. 
Published: November 2020

AbstractThe exponential growth in computer networks and network applications worldwide has been matched by a surge in cyberattacks. For this reason, datasets such as CSE-CIC-IDS2018 were created to train predictive models on network-based intrusion detection. These datasets are not meant to serve as repositories for signature-based detection systems, but rather to promote research on anomaly-based detection through various machine learning approaches. CSE-CIC-IDS2018 contains about 16,000,000 instances collected over the course of ten days. It is the most recent intrusion detection dataset that is big data, publicly available, and covers a wide range of attack types. This multi-class dataset has a class imbalance, with roughly 17% of the instances comprising attack (anomalous) traffic. Our survey work contributes several key findings. We determined that the best performance scores for each study, where available, were unexpectedly high overall, which may be due to overfitting. We also found that most of the works did not address class imbalance, the effects of which can bias results in a big data study. Lastly, we discovered that information on the data cleaning of CSE-CIC-IDS2018 was inadequate across the board, a finding that may indicate problems with reproducibility of experiments. In our survey, major research gaps have also been identified.
SARS-CoV-2 analysis on environmental surfaces collected in an intensive care unit: keeping Ernest Shackleton’s spirit
Authors: Escudero Dolores , Boga José Antonio , Fernández Javier , Forcelledo Lorena , Balboa Salvador , Albillos Rodrigo , Astola Iván , García-Prieto Emilio , Álvarez-Argüelles Marta Elena , Martín Gabriel , Jiménez Josu , Vázquez Fernando 
Published: November 2020

Summary
Background
Intensive care unit workers are at high risk of acquiring COVID-19 infection, especially when performing invasive techniques and certain procedures that generate aerosols (< 5 μm). Therefore, one of the objectives of the health systems should implement safety practices to minimize the risk of contagion among these health professionals. Monitoring environmental contamination of SARS-CoV-2 may help to determine the potential of the environment as a transmission medium in an area highly exposed to SARS-CoV-2, such as an intensive care unit. The objective of the study was to analyze the environmental contamination by SARS-CoV-2 on surfaces collected in an intensive care unit, which is dedicated exclusively to the care of patients with COVID-19 and equipped with negative pressure of – 10 Pa and an air change rate of 20 cycles per hour. Furthermore, all ICU workers were tested for COVID-19 by quantitative RT-PCR and ELISA methods.

Results
A total of 102 samples (72 collected with pre-moistened swabs used for collection of nasopharyngeal exudates and 30 with moistened wipes used in the environmental microbiological control of the food industry) were obtained from ventilators, monitors, perfusion pumps, bed rails, lab benches, containers of personal protective equipment, computer keyboards and mice, telephones, workers’ shoes, floor, and other areas of close contact with COVID-19 patients and healthcare professionals who cared for them. The analysis by quantitative RT-PCR showed no detection of SARS-CoV-2 genome in environmental samples collected by any of the two methods described. Furthermore, none of the 237 ICU workers was infected by the virus.

Conclusions
Presence of SARS-CoV-2 on the ICU surfaces could not be determined supporting that a strict cleaning protocol with sodium hypochlorite, a high air change rate, and a negative pressure in the ICU are effective in preventing environmental contamination. These facts together with the protection measures used could also explain the absence of contagion among staff inside ICUs.
Intratumoral and peritumoral radiomics analysis for preoperative Lauren classification in gastric cancer
Authors: Wang Xiao-Xiao , Ding Yi , Wang Si-Wen , Dong Di , Li Hai-Lin , Chen Jian , Hu Hui , Lu Chao , Tian Jie , Shan Xiu-Hong 
Published: November 2020

Abstract
Background
Preoperative prediction of the Lauren classification in gastric cancer (GC) is very important to the choice of therapy, the evaluation of prognosis, and the improvement of quality of life. However, there is not yet radiomics analysis concerning the prediction of Lauren classification straightly. In this study, a radiomic nomogram was developed to preoperatively differentiate Lauren diffuse type from intestinal type in GC.

Methods
A total of 539 GC patients were enrolled in this study and later randomly allocated to two cohorts at a 7:3 ratio for training and validation. Two sets of radiomic features were derived from tumor regions and peritumor regions on venous phase computed tomography (CT) images, respectively. With the least absolute shrinkage and selection operator logistic regression, a combined radiomic signature was constructed. Also, a tumor-based model and a peripheral ring-based model were built for comparison. Afterwards, a radiomic nomogram integrating the combined radiomic signature and clinical characteristics was developed. All the models were evaluated regarding classification ability and clinical usefulness.

Results
The combined radiomic signature achieved an area under receiver operating characteristic curve (AUC) of 0.715 (95% confidence interval [CI], 0.663–0.767) in the training cohort and 0.714 (95% CI, 0.636–0.792) in the validation cohort. The radiomic nomogram incorporating the combined radiomic signature, age, CT T stage, and CT N stage outperformed the other models with a training AUC of 0.745 (95% CI, 0.696–0.795) and a validation AUC of 0.758 (95% CI, 0.685–0.831). The significantly improved sensitivity of radiomic nomogram (0.765 and 0.793) indicated better identification of diffuse type GC patients. Further, calibration curves and decision curves demonstrated its great model fitness and clinical usefulness.

Conclusions
The radiomic nomogram involving the combined radiomic signature and clinical characteristics holds potential in differentiating Lauren diffuse type from intestinal type for reasonable clinical treatment strategy.
Actin filaments mediated root growth inhibition by changing their distribution under UV-B and hydrogen peroxide exposure in Arabidopsis
Authors: Du Meiting , Wang Yanhong , Chen Huize , Han Rong 
Published: November 2020

Abstract
Background
UV-B signaling in plants is mediated by UVR8, which interacts with transcriptional factors to induce root morphogenesis. However, research on the downstream molecules of UVR8 signaling in roots is still scarce. As a wide range of functional cytoskeletons, how actin filaments respond to UV-B-induced root morphogenesis has not been reported. The aim of this study was to investigate the effect of actin filaments on root morphogenesis under UV-B and hydrogen peroxide exposure in Arabidopsis.

Results
A Lifeact-Venus fusion protein was used to stain actin filaments in Arabidopsis. The results showed that UV-B inhibited hypocotyl and root elongation and caused an increase in H2O2 content only in the root but not in the hypocotyl. Additionally, the actin filaments in hypocotyls diffused under UV-B exposure but were gathered in a bundle under the control conditions in either Lifeact-Venus or uvr8 plants. Exogenous H2O2 inhibited root elongation in a dose-dependent manner. The actin filaments changed their distribution from filamentous to punctate in the root tips and mature regions at a lower concentration of H2O2 but aggregated into thick bundles with an abnormal orientation at H2O2 concentrations up to 2 mM. In the root elongation zone, the actin filament arrangement changed from lateral to longitudinal after exposure to H2O2. Actin filaments in the root tip and elongation zone were depolymerized into puncta under UV-B exposure, which showed the same tendency as the low-concentration treatments. The actin filaments were hardly filamentous in the maturation zone. The dynamics of actin filaments in the uvr8 group under UV-B exposure were close to those of the control group.

Conclusions
The results indicate that UV-B inhibited Arabidopsis hypocotyl elongation by reorganizing actin filaments from bundles to a loose arrangement, which was not related to H2O2. UV-B disrupted the dynamics of actin filaments by changing the H2O2 level in Arabidopsis roots. All these results provide an experimental basis for investigating the interaction of UV-B signaling with the cytoskeleton.
GIS-based gully erosion susceptibility modeling, adapting bivariate statistical method and AHP approach in Gombe town and environs Northeast Nigeria
Authors: Igwe Ogbonnaya , John Ugwuoke Ikechukwu , Solomon Onwuka , Obinna Ozioko 
Published: November 2020

AbstractGully erosion is a major environmental problem in Gombe town, a large area of land is becoming unsuitable for human settlement, hence the need for a gully erosion susceptibility map of the study area. To generate a gully inventory map, a detailed field exercise was carried out, during this investigation one hundred gullies were identified and studied extensively within the study area of about 550 km2. In addition to the mapped gullies, Google EarthPro with high-resolution imagery was used to locate the spatial extents of fifty (50) more gullies. Ten gully erosion predisposing factors were carefully selected considering the information obtained from literature, and multiple field survey of the study area, the factors include elevation, slope angle, curvature, aspect, topographic wetness index (TWI), soil texture, geology, drainage buffer, road buffer and landuse. In this study, a GIS-based Frequency Ratio (FR) and Analytical Hierarchy Process (AHP) models were employed to predict areas prone to gully erosion in Gombe town and environs. The result obtained from FR shows that drainage, soil texture, and slope have the highest correlation with gully occurrence, while the AHP model revealed that drainage buffer, soil texture, geology have a high correlation with the formation of a gully. Gully erosion susceptibility maps (GESM) were produced and reclassified into very high, high, moderate, and low zones. The overall accuracies of both models were tested utilizing area under the curve (AUC) values and gully density distribution.FR and AHP model have AUC values of 0.73 and 0.72 respectively, the outcome indicates that both models have high prediction accuracy. The gully erosion density distribution values revealed that gullies are concentrated in the very high susceptibility class and it decreases towards the low class, therefore the GESM produced using these models in this study area is reliable and can be used for land management and future planning.
An event based topic learning pipeline for neuroimaging literature mining
Authors: Chen Lihong , Yan Jianzhuo , Chen Jianhui , Sheng Ying , Xu Zhe , Mahmud Mufti 
Published: November 2020

AbstractNeuroimaging text mining extracts knowledge from neuroimaging texts and has received widespread attention. Topic learning is an important research focus of neuroimaging text mining. However, current neuroimaging topic learning researches mainly used traditional probability topic models to extract topics from literature and cannot obtain high-quality neuroimaging topics. The existing topic learning methods also cannot meet the requirements of topic learning oriented to full-text neuroimaging literature. In this paper, three types of neuroimaging research topic events are defined to describe the process and result of neuroimaging researches. An event based topic learning pipeline, called neuroimaging Event-BTM, is proposed to realize topic learning from full-text neuroimaging literature. The experimental results on the PLoS One data set show that the accuracy and completeness of the proposed method are significantly better than the existing main topic learning methods.
Characteristics of cytokines in the sciatic nerve stumps and DRGs after rat sciatic nerve crush injury
Authors: Zhang Rui-Rui , Chen Sai-Ling , Cheng Zhang-Chun , Shen Yin-Ying , Yi Sheng , Xu Hui 
Published: November 2020

Abstract
Background
Cytokines are essential cellular modulators of various physiological and pathological activities, including peripheral nerve repair and regeneration. However, the molecular changes of these cellular mediators after peripheral nerve injury are still unclear. This study aimed to identify cytokines critical for the regenerative process of injured peripheral nerves.

Methods
The sequencing data of the injured nerve stumps and the dorsal root ganglia (DRGs) of Sprague-Dawley (SD) rats subjected to sciatic nerve (SN) crush injury were analyzed to determine the expression patterns of genes coding for cytokines. PCR was used to validate the accuracy of the sequencing data.

Results
A total of 46, 52, and 54 upstream cytokines were differentially expressed in the SNs at 1 day, 4 days, and 7 days after nerve injury. A total of 25, 28, and 34 upstream cytokines were differentially expressed in the DRGs at these time points. The expression patterns of some essential upstream cytokines are displayed in a heatmap and were validated by PCR. Bioinformatic analysis of these differentially expressed upstream cytokines after nerve injury demonstrated that inflammatory and immune responses were significantly involved.

Conclusions
In summary, these findings provide an overview of the dynamic changes in cytokines in the SNs and DRGs at different time points after nerve crush injury in rats, elucidate the biological processes of differentially expressed cytokines, especially the important roles in inflammatory and immune responses after peripheral nerve injury, and thus might contribute to the identification of potential treatments for peripheral nerve repair and regeneration.
Innovative use of magnetic resonance imaging-guided focused ultrasound surgery for non-invasive breast cancer: a report of two cases
Authors: Matsutani Akiko , Ide Yoshimi , Miura Sakiko , Takimoto Masafumi , Amano Sadao , Nakamura Seigo 
Published: November 2020

Abstract
Objective
This report describes the first clinical experience with magnetic resonance imaging-guided focused ultrasound surgery (MRgFUS) using the ExAblate 2100 system for non-invasive breast cancer.

Methods
Two women with non-invasive breast cancer underwent MRgFUS treatment. One week after the MRgFUS treatment, US-guided vacuum-assisted biopsy was performed for the ablated lesions at the same time as breast-conserving surgery.

Results
The patients experienced good cosmetic outcomes and did not experience any severe adverse events, such as skin burns. Pathological examination of the surgical specimens revealed a few degenerated intraductal lesions around the breast biopsy markers.

Conclusion
Performing MRgFUS with the new ExAblate 2100 system appears to be safe and feasible. The histopathological results revealed that adequate ultrasound energy in the appropriate location can induce tumor necrosis.
Evaluation of in vivo wound healing activity of Moroccan Citrus reticulata peel extract
Authors: Dahmani Sanae , Chabir Rachida , Errachidi Faouzi , Berrada Wiam , Lansari Hafsa , Benidir Meryem , El Ghadraoui Lahsen , Bour Abdellatif 
Published: November 2020

Abstract
Background
Citrus reticulata is one of the most cultivated fruit with great benefits for humans in the world. Citrus reticulata peel has several biological activities within them hypoglycemic, hepatoprotective, antimicrobial and antioxidant. The present study emphasizes on the in vivo wound healing and in vitro antimicrobial and antioxidant activities of Citrus reticulata peel extract.

Methods
Forty albino mice (23–29 g) of either sex were divided into four groups. The test groups were treated with experimental ointment (0.5% and 10% of methanolic extract), negative control were treated with Vaseline and the positive control were treated with silver sulfadiazine. Burn wounds were induced on dorsal area of mice bodies. Wound area measurement was carried out every day during 22 days. Biochemical screening was performed to identify possible compounds. Antioxidant and antimicrobial activities were also determined.

Results
Significant wound healing activity was observed with topical application of Citrus reticulata peel extract. Wound area reduction at day 16 of treatment was 100% for both treated groups (0.5% and 10%) when compared to positive and negative control it was 100% and 98.32% respectively at day 22. Furthermore higher rate of wound contraction (100% on 16th day) was observed for both treated groups.
The result of biochemical screening showed that C. reticulata peel is characterized by highest amount of total polyphenols (13.19 mg/g), flavonoids (4.07 mg/g), vitamin C (13.20 mg/g), carotenoids (0.032 mg/g) and lowest content of macronutrients (Proteins: 0.40%, reducing sugars: 7.21%; lipids: 1.5%). Additionally C. reticulata peel exhibited remarkable antioxidant activity using DPPH and phosphomolybdate methods as well as the extract possess antimicrobial effect against pathogen bactria.

Conclusion
The findings from this research indicated that Citrus reticulata peel extract is effective in inhibiting the growth of pathogen bacteria and could be of therapeutic potentials for wound healing.