bluuebunny/crossref_metadata_2025_split huggingface dataset:
Structure of Vaginal Microbiome Community After Perineal Disinfection and Its Effects on Neonatal Oral Microbiome
Authors: Li Hongping , Nie Chuan , Xiao Bin , Chen Shaoyun , Yu Jieyang , Zhu Yuanfang 
Published: September 2020

Abstract
Background: Vaginal microbiota is not only an important source of bacterial colonization for neonates, but also plays a key role in maternal and neonatal health. In China, povidone iodine is used to disinfect vaginal during delivery. To date there has been no comprehensive study to investigate the vaginal microbiome composition after disinfection.Results: In this study, 27 women were recruited from Bao an Maternal and Child Health Hospital (Shenzhen, China). Vaginal samples before and after delivery were collected. Neonatal oral samples were also collected right after birth. Bacterial compositions of these study subjects were investigated using 16S rRNA sequencing of V3-V4 hyper-regions based on Hiseq 2500 platform. The results showed that vaginal microbiome during pregnancy were dominated by Lactobacillus spp. The identified microbiomes were separated into three community state types (CSTs), and a new CST (dominated by L. helveticus) was observed in this study. After disinfection, the relative abundance of Lactobacillus decreased and alpha diversity increased significantly. Moreover, most CST III and CST VI during pregnancy, both them dominated by Lactobacillus, shifted to CST IV in vaginal samples after disinfection. Additionally, the similar change pattern was observed in neonatal oral microbiome, and they overlapped with vaginal samples after disinfection in NMDS analysis.Conclusions: Perineal disinfection resulted in the decrease of genus Lactobacillus and increase of alpha diversity both in maternal vaginal microbiome and neonatal oral microbiome. In further, it is vital to understand the influence on maternal and neonatal health of vaginal microbiome community structure change after disinfection.
Rapid Detection of Toxoplasma gondii: Prevalence Investigation of T. gondii Infection Among Stray Cats and Dogs in Zhejiang Province Based on a New Loop-Mediated Isothermal Amplification-Lateral-Flow-Dipstick (LAMP-LFD) Device
Authors: Xue Yangji , Lu Shaohong , Kong Qingming , Ding Haojie , Ding Jianzu , Zheng Bin , Tong Qunbo , Zhuo Xunhui , Xie Chengzuo , Lou Di , Lv Hangjun 
Published: September 2020

Abstract
Background: Toxoplasma gondii (T. gondii) is worldwide spread caused Toxoplasmosis threatening warm-blooded animal and human health, especially for immunodeficient population and pregnant women. Simple and applicable diagnostic methods are urgently needed for the prevention of toxoplasmosis. The molecular diagnosis of T. gondii infection generally requires high technical skills, sophisticated equipment and a controlled lab environment.Methods: In this study, we developed a loop-mediated isothermal amplification-lateral-flow-dipstick (LAMP-LFD) assay that specifically targets the 529 bp for the detection of T. gondii infection in a new kind of portable device, which is universal, fast, user-friendliness, experimental sensitivity and low risk of aerosol contamination. Results: The detection limit of the LAMP-LFD assay is 1 fg of T. gondii DNA and no cross-reaction with other parasitic pathogens including Leishmania donovani, Plasmodium vivax, Cryptosporidium parvum, etc. In total, 318 stray cat and dog blood samples were collected from Deqing, Wenzhou, Yiwu, Lishui and Zhoushan cities in Zhejiang province, Eastern China. The current infection prevalence of T. gondii was 4.76% and 4.69% in stray cats and dogs respectively, detected by LAMP-LFD device.Conclusions: In conclusion, the established LAMP-LFD was an efficient and avoidable aerosol-contaminated device that can detect 1 fg genomic DNA of T. gondii, and suitable for T. gondii detection in the basic medical institution and even in field areas.
MiR-30c regulates metastasis and polarity reversal of tumor cell clusters by targeting MTDH in invasive micropapillary carcinoma of the breast
Authors: Han Yunwei , Li Weidong , Zhi Renyong , Ma Gui , Gao Ang , Wu Kailiang , Song Yawen , Sun Hui , Zhao Dan , Yang Yiling , Liu Fangfang , Gu Feng , Guo Xiaojing , Dong Jintang , Li Shuai , Fu Li 
Published: September 2020

Abstract
Purpose

Invasive micropapillary carcinoma (IMPC) of the breast has a high propensity for lymphovascular invasion and axillary lymph node metastasis and displays an ‘inside-out’ growth pattern, but the molecular mechanism of metastasis and tumor cell polarity reversal in IMPC is unclear.
Methods and Patients:

Luciferase reporter assays and western blotting were performed to confirm that the MTDH 3’UTR bound to miR-30c. Growth curves, tumor sphere formation assays, Transwell migration and invasion assays, mouse xenograft model and immunofluorescence were evaluated to investigate the effects of miR-30c and MTDH. MiRNA in situ hybridization (ISH) and immunohistochemistry (IHC) were carried out on IMPC patient tissues for miR-30c and MTDH expression, respectively.
Results

We found that miR-30c could directly target the MTDH (metadherin) 3’UTR. MiR-30c overexpression inhibited breast cancer cell proliferation, invasion and metastasis by targeting MTDH in vitro and in vivo. Moreover, miR-30c regulated IMPC cell polarity reversal by targeting MTDH. By in situ hybridization and immunohistochemistry analyses, miR-30c and MTDH were significantly correlated with tumor size, lymph nodule status and tumor grade, the ‘inside-out’ growth pattern, overall survival (OS) and disease-free survival (DFS) in IMPC patients (p < 0.05).
Conclusions

Overall, miR-30c overexpression could inhibit breast cancer cell proliferation and metastasis and regulate polarity reversal by targeting MTDH.
The influence of medical insurance and social security cards on the floating population's settlement intention
Authors: Li Yulin , Huang Lingling , Xiang Li , Dou Dongmei 
Published: September 2020

Abstract
Background: There are many studies on the influencing factors of floating population's intention to settle down. Medical insurance and social security cards have an important guarantee for the floating population to live a stable life in the current residence, but there are limited studies focused on the influence of medical insurance and social security cards on their settlement intention. Therefore, the purpose of this paper is to study the influence of medical insurance and social security card on the settlement intention of floating population, so as to create better living and working conditions for floating population and improve their happiness in their current place of residence. Methods: Based on the survey data of China's floating population dynamic monitoring in 2017, we explored the influence of medical insurance (urban employee basic medical insurance) and social security cards on the floating population's settlement intention with binary logistic regression and structural equation model. Additionally, this study was also to examine the comprehensive causal relationship, with social integration as the mediator variable. Results: The floating population's settlement intention on participating in urban employee basic medical insurance is 23.2 percent higher than those who did not participate. Whether to apply for personal social security cards is related to the settlement intention. The standardized regression coefficients among social insurance and security, social integration, and settlement intention are positive values, and the Z values ​​of the overall effect, indirect effect, and direct effect are all greater than 1.96, and the confidence interval of the indirect effect does not include 0. The article found that this model is a partial intermediary, with an intermediary ratio of 10.66 percent. Conclusions: The article highlights the important impact of urban workers' medical insurance and individual social security cards on the floating population. The results of this study may provide some reference for the government to formulate relevant policies.
Plasma Levels of Phosphorylated Tau 181 Are Associated With Cerebral Metabolic Dysfunction in Cognitively Impaired Individuals
Authors: Lussier Firoza Z , Benedet Andréa L. , Therriault Joseph , Pascoal Tharick A. , Tissot Cécile , Chamoun Mira , Mathotaarachchi Sulantha , Savard Melissa , Ashton Nicholas J. , Karikari Thomas K. , Rodriguez Juan Lantero , Snellman Anniina , Bezgin Gleb , Kang Min Su , Arias Jaime Fernandez , Wang Yi-Ting , Gauthier Serge , Zetterberg Henrik , Blennow Kaj , Rosa-Neto Pedro 
Published: September 2020

Abstract
BackgroundAlzheimer’s disease (AD) biomarkers are primarily evaluated through MRI, PET, and CSF methods in order to diagnose and monitor disease. Recently, advances in the assessment of blood-based biomarkers have shown promise for simple, inexpensive, accessible, and minimally invasive tools with diagnostic and prognostic value for AD. Most recently, plasma phosphorylated tau181 (p-tau181) has shown excellent performance. The relationship between plasma p-tau181 and cerebral metabolic dysfunction assessed by [18F]FDG PET in AD is still unknown.MethodsThis study was performed on a total of 892 individuals (297 cognitively unimpaired; 595 cognitively impaired) from the ADNI cohort. Plasma p-tau181 was assessed using single molecular array (Simoa) technology and metabolic dysfunction was indexed by [18F]FDG PET. Cross-sectional associations between plasma and CSF p-tau181 and [18F]FDG were assessed using voxelwise linear regression models, with individuals stratified by diagnostic group and by Aβ status. Associations between baseline plasma p-tau181 and longitudinal rate of brain metabolic decline were also assessed in a subset (n=389) of individuals using correlations and voxelwise regression models.ResultsPlasma p-tau181 was elevated in Aβ+ and cognitively impaired individuals as well as in APOE ε4 carriers, and was significantly associated with age, worse cognitive performance, and CSF p-tau181. Cross-sectional analyses showed strong associations between plasma p-tau181 and [18F]FDG PET in Aβ+ and cognitively impaired individuals. Voxelwise longitudinal analyses showed that baseline plasma p-tau181 concentrations were significantly associated with annual rates of metabolic decline only in cognitively impaired individuals, bilaterally in the medial and lateral temporal lobes.ConclusionsThe associations between plasma p-tau181 and reduced brain metabolism, primarily in cognitively impaired and in Aβ+ individuals, supports the use of plasma p-tau181 as a simple, low-cost, minimally invasive, and accessible tool to both assess current and predict future metabolic dysfunction associated with AD, comparatively to PET, MRI, and CSF methods.
Early infant diagnosis and associated factors among HIV exposed infants in West Shoa Zone, Ethiopia: A mixed methods study
Authors: Debelew Gurmesa Tura , Fana Bontu Berhanu , Habte Mahilet Birhanu 
Published: September 2020

Abstract
Background: The World Health Organization recommends testing of all HIV exposed infants at 4–6 weeks of age to optimize detection of intrauterine, intrapartum, and early postnatal HIV transmissions. However, the global coverage of early infant diagnosis remains low. With less than 25% national coverage, the progress of this early diagnosis in Ethiopia is one of the slowest in the World. Furthermore, local studies are limited to determine the status and identify the associated factors in many parts of the country in general and in West Shoa Zone in particular. Thus, this study aimed to fill this gap.Methods: A mixed method study by using retrospective record review of four years facility data of 342 mother-infant pairs supplemented by qualitative method was conducted in West Shoa Zone, Oromia Region, Ethiopia in 2018. The quantitative data related to maternal and HIV exposed infants’ cares were collected by using questionnaire. The qualitative data related to facility related barriers were collected by key-informants interview with selected mothers and service providers. Factors associated with early infant diagnosis of HIV infection were identified by using multivariable logistic regression analysis. The qualitative data were analyzed thematically and triangulated with the quantitative findings. Results: The status of early infant diagnosis was 58.5% (95%CI: 53.3%, 63.7%). Attending secondary education or above (AOR=2.41; 95%CI: 1.54, 3.28), having <4 living children (AOR=4.76, 95%CI: 02.56, 9.09), knowing HIV sero-status during or before pregnancy (AOR=6.24, 95%CI: 2.40, 10.08) and sero-status disclosure (AOR=8.30, 95%CI: 3.30, 20.60) were significantly associated with early infant diagnosis. Attending ANC visit (AOR=5.32; 95%CI: 2.53, 8.11), giving birth in health facility (AOR=62; 95%CI: 3.39, 11.85) and Neverapine provision at enrolment (AOR=6.05; 95%CI: 2.48, 14.73) were also significantly associated with early infant diagnosis of HIV infection.Conclusion: The status of early infant diagnosis in the study area is low to achieve the national target of 95% coverage by 2020. Maternal socio-demography, maternal behavior in knowing sero-status and disclosing and using services during pregnancy and delivery were among the determinants of early infant diagnosis. Promoting ANC and PMTCT cascade through behavioral interventions and fulfilling the essential equipments and supplies are required.
Cointegration Analysis and Forecasting of the Export Function of Bangladesh Using the Error Correction Model
Authors: Islam K M Saemon , Biswas Gautam Kumar 
Published: September 2020

Abstract
In this paper, we examined the relationship between the growth of the Gross Domestic Product of the United States, the export value index, and the export of Bangladesh over 37 years between 1980 and 2016. The results of our preliminary tests showed that there was indeed a long-run relationship between these variables. Based on our preliminary analysis, we employed an error-correction model to identify the relationship between the variables. The error-correction term with the expected negative sign was statistically significant, and it confirmed that in the case of disequilibrium, the convergence towards the equilibrium happened in the subsequent periods. Additionally, the econometric estimates exhibited that the two-period lagged values of the growth in export of Bangladesh and the growth of the Gross Domestic Product of the United States were also statistically significant.JEL Classification: C22, C5, F41
Post-Financial Crises Puzzle of Economic Growth in Low and Lower-Middle Income Countries: Evidence from Static and Dynamic Panel Estimations
Authors: shafiq Mohammad m , ADELEYE Bosede Ngozi 
Published: September 2020

Abstract
This paper investigates economic growth in 65 low and lower-middle-income countries from 2010 to 2017. Results from static and dynamic panel data techniques show that foreign direct investment (FDI), capital formation, and labor force significantly increase economic growth while government expenditure decreases growth. Mobile phone subscription reveals no significant impact on growth at aggregate levels. Furthermore, analyses at the income group level reveal substantial differences between the determinants of economic growth in low and lower-middle-income countries. For instance, FDI is a significant predictor of growth in low-income countries, but not in lower-middle-income countries. Similarly, government expenditure and mobile cellular subscriptions are not significant predictors in low-income countries relative to lower-middle-income countries. Interestingly, capital formation and labor force are the two common significant predictors across low and lower-income countries.
Clinical Data From the Real World: Efficacy Analysis of Ceritinib (450mg) in ALK-Rearrangement Non-Small Cell Lung Cancer Patients with Brain Metastases in China
Authors: Qiu Zhi Xin , Liu Chunrong , Wang Ke , Huang Meijuan , Yu Min , Xian Xinhong 
Published: September 2020

Abstract
Objectives: The real-world intracranial efficacy data of ceritinib at a dose of 450mg quaque die (QD, once a day) are still unavailable, thus this study aims to explore the intracranial efficacy of ceritinib (450mg QD) in anaplastic lymphoma kinase (ALK)-rearrangement non-small cell lung cancer (NSCLC) patients in China.Materials and Methods: The intracranial efficacy [objective response rate (ORR) and disease control rate (DCR)] was evaluated according to the Response Assessment in Neuro-Oncology (RANO) standard, along with progression-free survival (PFS) and adverse events (AEs) obtained through follow-ups.Results: A total of 57 ALK-rearrangement NSCLC patients with brain metastases (BM) were enrolled in this study. Among them, 53 patients experienced progression at baseline during or after prior crizotinib, and 24 patients received prior brain radiotherapy. The intracranial ORR and DCR were 73.7% and 93.0%, respectively. The median intracranial PFS in patients reaching the endpoint was 8.75 months; while that in all patients was not reached and predicted to be not evaluable (NE) (95% CI: 12.9-NE). The estimated 12-month event-free probability (EFP) of intracranial lesions was 68.1%. Subgroup analysis showed the estimated 12-month EFP of intracranial lesions was relatively higher in patients with prior brain radiotherapy (93.8% vs 47.1%, P=0.0006). Additionally, we reported a 74-year-old female ALK-rearrangement NSCLC patient with BM achieved continuous response (intracranial PFS: 12.9 months) to ceritinib reduced to 150mg QD due to intolerable AE and administered for 7.5 months.Conclusion: Ceritinib administered at a dose of 450mg QD to ALK-rearrangement NSCLC patients with BM in China demonstrates superior ORR and DCR, as well as PFS and EFP that are expected to be improved. Especially the estimated 12-month EFP of intracranial lesions was improved in patients with prior brain radiotherapy.
Comparative Transcriptomic Analysis of Gonad Development and Renewal of Ovoviviparous Black Rockfish (Sebastes Schlegelii)
Authors: Li Jianshaung , Wen Haishen , Lyu Likang , Li Yun , Wang Xiaojie , Zhang Ying , Yao Yijia , Qi Xin 
Published: September 2020

Abstract
Background: Black rockfish (Sebastes schlegelii) has an ovoviviparous reproductive pattern and long-term sperm storage, which resulting in asynchronous gonadal development between the sexes. While the comprehensive understanding of gonad development of black rockfish has not been well studied. Here, we study the gonad development and germ cell renewal by histology and RNA-seq.Results: In this study, RNA-seq was performed on both testis and ovary to characterize key pathways and genes during development and gamete maturation in black rockfish. Different expression genes (DEGs) were identified and annotated in 4 comparisons (F_III vs F_IV, F_IV vs F_V, M_III vs M_IV and M_IV vs M_V). Based on enriched analysis of DEGs in testis, 11 and 14 significantly enriched Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were mapped in M_III vs M_IV group and M_IV vs M_V group, respectively. And DEGs in ovary development periods were also classified into 10 biological function group. The results of the q-PCR expression pattern of the selected genes was significantly correlated with the RNA-Seq results, implying the reliability and accuracy of the RNA-Seq analysis.Conclusion: The categories intercellular interaction and cytoskeleton, molecule amplification and repairment in cell cycle were revealed to be crucial in testis development and spermatogenesis along with a series of metabolite biosynthesis. Our results provided a comprehensive insight into the black rockfish gonad development for further study of reproductive physiology and molecular biology in ovoviviparity.
Locoregional treatment in de novo metastatic breast cancer: a retrospective cohort study
Authors: Jianna Sun , Lingjun Kong , Nana Feng , Hong Liu , Chongxi Ren 
Published: September 2020

Abstract
Background: In an earlier analysis of this cohort study, local therapy based on surgical resection of the primary tumor might confer a survival benefit in women with de novo metastatic breast cancer (dnMBC). Here we report the survival outcomes of locoregional treatment (LRT), focusing on the association of surgical timings and surgical margins with survival in these patients. Methods: The retrospective study included patients with dnMBC in two Chinese tertiary hospitals, between March 1, 2007, and December 31, 2017. Overall survival (OS) was evaluated by means of a stratified log-rank test and summarized with the use of Kaplan–Meier methods. Results: A total of 153 patients were included, of whom 87 underwent LRT and 66 systemic therapy alone (STA). LRT showed a significant OS benefit over STA (HR, 0.47; 95% CI, 0.33 to 0.69; p<.0001). Median OS of LRT group and STA group were 42 months (95% CI, 35.0 to 48.9 months) and 21 months (95% CI, 16.1 to 25.9 months), respectively. The benefit was consistent across most subgroups. The OS of patients undergoing surgery was better than that of patients without surgery (HR, 0.48; 95% CI, 0.33 to 0.70; p=.0001), and there was difference in survival improvement at different surgical timings (surgery before chemotherapy, during chemotherapy and after chemotherapy) (HR, 0.79; 95% CI, 0.65 to 0.95; p=.013). The survival benefit of surgery after chemotherapy was the most, followed by surgery during chemotherapy (Median 56 months, 95% CI, 40.8 to 71.2 months). Moreover, compared with patients with positive margins, the OS of patients with negative margins was significantly improved (HR, 2.35; 95% CI, 1.65 to 3.35; p<.0001), with a median OS of 56 months (95% CI, 45.9 to 66.1 months). Conclusions: Our results suggest that LRT is associated with improved OS in women with dnMBC, and patients who had surgery after or during systemic chemotherapy with negative surgical margins, are expected to benefit more.
Combining lung ultrasound and wells score for diagnosing pulmonary embolism in critically ill COVID-19 patients
Authors: Zotzmann Viviane , Lang Corinna Nadine , Wengenmayer Tobias , Bemtgen Xavier , Schmid Bonaventura , Mueller-Peltzer Katharina , Supady Alexander , Bode Christoph , Duerschmied Daniel , Staudacher Dawid L 
Published: September 2020

Abstract
Introduction: Subpleural consolidations have been found in lung ultrasound in patients with COVID-19, possibly deriving from pulmonary embolism (PE). The diagnostic utility of impact of lung ultrasound in critical-ill patients with COVID-19 for PE diagnostics however is unclear.Methods: We retrospectively evaluated all SARS-CoV2-associated ARDS patients admitted to our ICU between March 8th and May 31th 2020. They were enrolled in this study, when a lung ultrasound and a computed tomography pulmonary angiography (CTPA) were documented. In addition, wells score was calculated to estimate the probability of PE. The CTPA was used as the gold standard for the detection of PE. Results: Twenty patients met the inclusion criteria. In 12/20 patients (60%) (sub-) segmental PE were detected by CT-angiography. Lung ultrasound found subpleural consolidations in 90% of patients. PE-typical large supleural consolidations with a size ≥1cm were detectable in 65% of patients and were significant more frequent in patients with PE compared to those without (p=0.035). Large consolidations predicted PE with a sensitivity of 77% and a specificity of 71%. The Wells score was significantly higher in patients with PE compared to those without (2.7±0.8 and 1.7±0.5, respectively, p=0.042) and predicted PE with an AUC of 0.81. When combining the two modalities, comparing patients with considered/probable PE using LUS plus a Wells score ≥2 to patients with possible/unlikely PE in LUS plus a Wells score <2, PE could be predicted with a sensitivity of 100% and a specificity of 80%.Conclusion: Large consolidations detected in lung ultrasound were found frequently in COVID-19 ARDS patients with pulmonary embolism. In combination with a Wells score>2, this might indicate a high-risk for PE in COVID-19.