bluuebunny/crossref_metadata_2025_split huggingface dataset:
Low expression of lncRNA CTB-104H12.5 induces resistance to adriamycin in HER2 negative breast cancer by downregulating GAS7C
Authors: Nian Wei-Qi , He Yong-Peng , Xue Wei-Wei , Tang Wan-Yan , Li Zheng , Wu Hai-bo , Ran Jing , Lin Chang-Hai , Wang En-Wen , Zhang Haiwei 
Published: July 2020

Abstract
Background

Adriamycin-based neoadjuvant chemotherapy is widely used in HER2- breast cancer patients, however, some patients have no response or acquire resistance to these regimens. Therefore, it is important to screen patients who respond to adriamycin with biomarkers.

Methods

A human lncRNA microarray analysis followed by a quantitative real-time polymerase chain reaction (qRT-PCR) assay was utilized to identify the differentially expressed lncRNAs. The proliferation viability of cells transfected with or without CTB 104H12.5 was measured by CCK-8 and clone formation assay, cell apoptosis and cell cycle were evaluated by Flow cytometry. And the expression of RNA and proteins was tested by qRT-PCR and Western blot, respectively.

Results

Ten whole blood samples from HER2- breast cancer patients who received docetaxel combined with adriamycin regimen were enrolled, a human lncRNA microarray analysis showed that the low expression of CTB-104H12.5 was associated unfavorable prognosis. And the mRNA and protein level of CTB-104H12.5 is much lower in MCF-7/ADR cells than that of MCF-7 cells while CTB-104H12.5 knockdown in MCF cells induces cell resistance to adriamycin, while CTB-104H12.5 overexpression in MCF7/ADR cells reversed their resistance to adriamycin. Also, the mRNA and protein levels of GAS7C were associated with CTB-104H12.5.

Conclusions

Low expression of CTB-10412.5 predicted resistance to adriamycin in HER2 negative breast cancer, which may serve as a potential biomarker to dynamically monitor adriamycin resistance for breast cancer patients.
Law and Public Relations in Indonesia: Viewed from the Theory of John Henry Merryman on Strategies of Legal Development
Authors: Djatmiko Wahju Prijo 
Published: July 2020

Principally, legal development is a sustainable development, its function as human interest protection, legal aims to reach an order and balance. Order in society guarantees the protection on human interest. Even though, the development on law is directed to create order in society, meaning law and society are interconnected, there are still plenty of legal products that are not able to meet people needs, and one of them is the judicial review on the Act no. 19 year 2013 on Protection and Enforcement to Farmers. This reflects that the Act does not represent social factors. This shows no harmony and benefit connections between the Act no.19 year 2013 as written legal product and society. This phenomenon, then, is analyzed from Theory of John Henry Merryman on Legal Development Strategy (Orthodox and Responsive). The process of making a responsive legal product is a participative one meaning that the process involves greatly the participation of society through social groups and individual in community. Reversely, orthodox legal product is characterized by its centralistic process in which state institutions dominate the process, especially the authority of executives.
Determination of optimum operational conditions for the removal of 2-MIB from drinking water by peroxone process: a pilot-scale study
Authors: Fakioglu M. , Gulhan H. , Ozgun H. , Ersahin M. E. , Ozturk I. 
Published: July 2020

Abstract
Taste and odor in drinking water are one of the main problems of the water supply and treatment sector. Peroxone is an effective advanced oxidation process, which combines ozone with hydrogen peroxide to create hydroxyl radicals that decompose organic compounds. 2-Methylisoborneol (2-MIB) is one of the significant taste and odor causing compounds, which can be removed with the peroxone process. In this study, removal of a 2-MIB compound by peroxone process was investigated in a pilot-scale treatment plant and optimum operational conditions were determined. For safety reasons, it is important that residual O3 and H2O2 concentrations in the water leaving the reactor should not exceed 0.1 and 0.5 mg/L, respectively. Results indicate that while dissolved ozone concentration was below the indicated limit for all experiments, concentrations over 0.5 mg/L residual H2O2 were observed during the experiments with an H2O2:O3 ratio of 0.5. This limit exceedance affected the decision on the ideal peroxone ratio along with the 2-MIB removal results. Therefore; optimum H2O2:O3 ratio was determined as 0.3. 2-MIB removal efficiency of 81% was achieved at the optimum H2O2:O3 ratio with a contact time of 15 min. According to the results, 2-MIB removal rate had a linear correlation with the contact time.
Simultaneous Determination of Penicillin G and Chloramphenicol in Milk by a Magnetic Nanoparticle-Based Fluorescent Immunoassay
Authors: Atanasova Milka , Ivanov Yavor , Zvereva Elena , Zherdev Anatoly , Godjevargova Tzonka 
Published: July 2020

Background:
Antibiotic residues are a problem of increasing importance and have direct consequences for human and animal health. The frequent use of antibiotics in veterinary practice causes their excretion in milk in dairy cattle. This way, they can easily enter the human body through the consumption of milk and dairy products.

Objectives:
This induces the need for accurate and sensitive methods to monitor antibiotic levels in milk. The aim of this study was to develop a rapid and sensitive magnetic nanoparticle-based fluorescence immunoassay for the simultaneous detection of chloramphenicol and penicillin G in milk.

Methods:
Magnetic nanoparticles were synthesized and functionalized with (3-aminopropyl)triethoxysilane. Chloramphenicol-Ovalbumin and Chloramphenicol-Ovalbumin-Fluorescein-5-isothiocyanate conjugates were prepared. Penicillin G – ATTO 633 fluorescent conjugate was synthesized. Antibodies against chloramphenicol and penicillin G were immobilized onto the magnetic nanoparticles. The competitive fluorescent immunoassay was developed. The optimal concentration of the antibody-magnetic nanoparticles and the fluorescent conjugates for the assay was determined. The calibration curves for the antibiotics in buffer and milk were plotted. Fluorescent immunoassay for the simultaneous determination of chloramphenicol and penicillin G in milk was developed.

Results:
The limit of detection by the simultaneous immunoassay of chloramphenicol and penicillin G in milk was 0.85 ng/mL and 1.6 ng/mL, respectively. The recovery of different concentrations of chloramphenicol and penicillin G in milk samples varied from 98% to 106%.

Conclusions:
A rapid and sensitive magnetic nanoparticle-based immunofluorescent assay for the simultaneous determination of chloramphenicol and penicillin G in milk was developed. The magnetic nanoparticles ensured rapid and easy procedure.
Correlation between Serum Vitamin A and D Levels in Acute Phase Ischemic Stroke and Clinical Outcome
Authors: Kadri Alfansuri , Sjahrir Hasan , Sembiring Rosita J. , Ichwan Muhammad 
Published: July 2020

Background:
In the last decade, a number of studies have examined the relationship between serum vitamin D concentration and the risk of cerebrovascular events. Besides vitamin D, the latest evidence shows that vitamin A is also a risk factor for cerebrovascular disease. Vitamin A and its derivatives act biologically via specific nuclear receptors that regulate gene transcription. Vitamin A receptors can also interact with other nuclear receptors that have neuroprotective effects such as vitamin D, against stroke. Although many studies suggested the synergism of vitamin A and D, there is still no study that evaluates their levels simultaneously in acute phase ischemic stroke, and the relationship to outcome.

Objective:
The objective of this study was to analyze the correlation between serum vitamin A and D levels on admission in Acute Ischemic Stroke patients and clinical outcome by using the National Institutes of Health Stroke Scale (NIHSS).

Methods:
A prospective cohort study was conducted, and samples were followed since the diagnosis of acute-phase Ischemic Stroke was established until the clinical outcome of day 14 after stroke onset. A total of 50 subjects enrolled for this study would be examined for serum levels of vitamins A and D on admission, and on the 14th day were assessed for NIHSS as a clinical outcome.

Results:
From 50 research subjects, the mean of vitamin A and D level in the acute phase of Ischemic Stroke was 463.35 ± 116.97 µg/L and 21.65 ± 6.51 ng/mL, respectively. By using the Spearman’s correlation test, it was found that the acute phase vitamin A level and NIHSS on day 14 had a significant and strong correlation with p = 0.045 (r = -0.672). Along with it, vitamin D serum levels and NIHSS also had a significant and strong correlation with p = 0.026 (r = -0.754). Both of these results showed that vitamin A and D had an inverse association with NIHSS, meaning that the higher vitamin A and D serum levels, the better the clinical outcome would be.

Conclusion:
Both serum vitamin A and D levels in the acute phase of Ischemic Stroke was correlated strongly with short time clinical outcome. The higher vitamin A and D serum levels in the acute phase, the better the clinical outcome would be for Ischemic Stroke patients.
Investigating Learning Challenges from the Perspective of Nursing Students and Educators at a University in Casablanca, Morocco
Authors: Gouifrane Rachid , Lajane Halima , Benmokhtar Said , Dehbi Fatima , Radid Mohamed 
Published: July 2020

Background:Whether in an academic or clinical setting, many students often consider nursing studies to be difficult. Identifying learning challenges could help to improve the quality of the training and students’ skills.Objective:This study aimed to identify the main learning challenges of nursing students.Methods:The study utilised a two-phase mixed methods design. First, a focus group discussion with nine teachers and tutors was conducted to explore the learning challenges observed by staff. Then, based on the results, a questionnaire was developed for 54 students to explore and estimate the frequency of learning challenges perceived by them.Results:The data analysis confirmed 15 major learning challenges, including cognitive challenges, consisting of theoretical gaps, and challenges related to clinical reasoning, and non-cognitive procedural and behavioural challenges. Challenges related to clinical reasoning, such as the difficulty in developing care plans (100%) and choosing care actions (90.2%), remain the most frequent regardless of the level of study.Conclusion:Supervisors should consider students’ learning challenges when designing, developing and implementing their educational interventions.
Psychological Stress in Final Year Nursing Students: The role of clinical environment
Authors: Nasir Norhayati , Abdul Mulud Zamzaliza 
Published: July 2020

Past studies have shown that nursing students are stressed. The purpose of this study is to examine the clinical environment factors associated with stress among final year nursing students by adapting the Transactional Stress and Coping Model (Lazarus and Folkman, 1984). The cross-sectional study design was used, data were collected over two months in 2019. A self-administered questionnaire, used to collect data. 420 final year students recruited using a simple random sampling technique. Based on one-way ANOVA, there was a significant mean difference of primary appraisal score and secondary appraisal score. Resilience level was significantly associated with the level of stress. Keywords: nursing students, stress, resilience. eISSN: 2398-4287© 2020. The Authors. Published for AMER ABRA cE-Bsby e-International Publishing House, Ltd., UK. This is an open access article under the CC BYNC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). Peer–review under responsibility of AMER (Association of Malaysian Environment-Behaviour Researchers), ABRA (Association of Behavioural Researchers on Asians) and cE-Bs (Centre for Environment-Behaviour Studies), Faculty of Architecture, Planning & Surveying, UniversitiTeknologi MARA, Malaysia. DOI:
Expatriates’ Psychological Distress Components of Malaysian Construction Company in Host Country
Authors: Zainol Halmi , A Tambi Abdul Malek , Ali Nor Eeda , Azami Hazirah 
Published: July 2020

Psychological distress is pertaining to anything leads to human well-being and satisfaction of the individual. Individual adjustment is a degree of psychological distress that an expatriate faces during an assignment in the host country. Unhappiness with the organisation will contribute to leaving for a prospective employer. Psychological influences such as interaction, reward and, social life contribute to the success of the expatriate. Content analysis was used in the study. The study aimed to determine the factors which led to expatriate's physiological distress. The study had found that there was a need for psychological adjustment while working in the host country. Keywords: Psychological distress; Expatriate; ConstructioneISSN: 2398-4287© 2020. The Authors. Published for AMER ABRA cE-Bs by e-International Publishing House, Ltd., UK. This is an open-access article under the CC BYNC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). Peer-review under responsibility of AMER (Association of Malaysian Environment-Behaviour Researchers), ABRA (Association of Behavioural Researchers on Asians) and cE-Bs (Centre for Environment-Behaviour Studies), Faculty of Architecture, Planning & Surveying, Universiti Teknologi MARA, Malaysia. DOI: https://doi.org/10.21834/ebpj.v5i14.2233
Developing a Demographic, Human Capital Values and Economic Profiling of Rohingya Refugees Workers in Malaysia
Authors: Ghazali Mohd Safwan , Tan Peck Leong , Abd Rashid Arlinah , Shariff Sazlin Suhalmie 
Published: July 2020

One of the most important trends with regards to forced migration is the growing number of refugees hosted in developing countries like Turkey, Bangladesh, and Malaysia. The unnoticed facts, but the truth is Rohingya ethnic are the longest staying refugees in Malaysia. In line with Sustainable Development Goals (SDGs), whose primary objectives to leave no-one behind, protect the environment, and ensure peace, investigating who these people are in terms of their livelihood, skills, and other economic characteristics is imperative. The data was collected through a face-to-face structured questionnaire. The study findings suggested some valuable information to assist the government in delivering inclusive refugees' rights to work. Keywords: Rohingya Refugees; Economic Profiling; Inclusive Employment; Sustainable Development Goals. eISSN: 2398-4287© 2020. The Authors. Published for AMER ABRA cE-Bs by e-International Publishing House, Ltd., UK. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). Peer-review under responsibility of AMER (Association of Malaysian Environment-Behaviour Researchers), ABRA (Association of Behavioural Researchers on Asians) and cE-Bs (Centre for Environment-Behaviour Studies), Faculty of Architecture, Planning & Surveying, Universiti Teknologi MARA, Malaysia. DOI: https://doi.org/10.21834/ebpj.v5i14.2242
Coping Strategy and Depression among Caregivers of Cancer Patients Receiving Palliative Care
Authors: Nor Hadi Noor Melissa , Ahmad Fazlina , Isa Mohamad Rodi , Razali Salmi 
Published: July 2020

Major depressive disorder (MDD) put caregivers of palliative cancer patients (CPCP) and the patients at risk for various physical and psychological complications. This study aimed to determine the prevalence of MDD and its contributing factors among CPCP. The diagnosis of MDD was determined using the Mini International Neuropsychiatric Inventory. Pro forma and validated questionnaires were used to assess sociodemography, caregiving and patient factors, coping strategy, and social support. About 6% of CPCP had MDD. Predictors for MDD were coping by behavioural disengagement and positive reframing. These coping strategies are crucial for CPCP to have optimum mental health status. Keywords: Caregiver, Cancer, Palliative, Depression, Coping Strategy. eISSN: 2398-4287© 2020. The Authors. Published for AMER ABRA cE-Bs by e-International Publishing House, Ltd., UK. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). Peer-review under responsibility of AMER (Association of Malaysian Environment-Behaviour Researchers), ABRA (Association of Behavioural Researchers on Asians) and cE-Bs (Centre for Environment-Behaviour Studies), Faculty of Architecture, Planning & Surveying, Universiti Teknologi MARA, Malaysia. DOI: https://doi.org/10.21834/ebpj.v5i14.2334
Guideline Recommendations for Oral Care After Acquired Brain Injury: Protocol for a Systematic Review
Authors: Gurgel-Juarez Nalia , Perrier Marie-France , Hoffmann Tammy , Lannin Natasha , Jolliffe Laura , Lee Rachel , Brosseau Lucie , Flowers Heather 
Published: July 2020

Background
Oral care is important to prevent buccal and systemic infections after an acquired brain injury (ABI). Despite recent advancements in the development of ABI clinical practice guidelines, recommendations for specific clinical processes and actions to attain adequate oral care often lack information.

Objective
This systematic review will (1) identify relevant ABI clinical practice guidelines and (2) appraise the oral care recommendations existing in the selected guidelines.

Methods
A search strategy was developed based on a recent systematic review of clinical practice guidelines for ABI. The protocol includes a search of MEDLINE, EMBASE, and DynaMed Plus databases, as well as organizational and best-practice websites and reference lists of accepted guidelines. Search terms will include medical subject headings and user-defined terms. Guideline appraisal will involve the Appraisal of Guidelines for Research and Evaluation II ratings, followed by a descriptive synopsis for oral care recommendations according to the National Health and Medical Research Council evidence levels.

Results
This project started in April 2019, when we developed the search strategy. The preliminary search of databases and websites yielded 863 and 787 citations, respectively, for a total of 1650 citations. Data collection will start in August 2020 and we expect to begin disseminating the results in May 2021.

Conclusions
Nursing staff may not have detailed recommendations on how to provide oral care for neurologically impaired patients. The findings of this review will explore the evidence for oral care in existing guidelines and improve outcomes for patients with ABI. We expect to provide adequate orientations to clinicians, inform policy and guidelines for best practices, and contribute to future directions for research in the ABI realm.

International Registered Report Identifier (IRRID)
PRR1-10.2196/17249
Digital Mental Health Resources for Asylum Seekers, Refugees, and Immigrants: Protocol for a Scoping Review
Authors: Raphiphatthana Buaphrao , Maulana Herdiyan , Howarth Timothy , Gardner Karen , Nagel Tricia 
Published: July 2020

Background
Asylum seekers, refugees, and immigrants experience a number of risk factors for mental health problems. However, in comparison to the host population, these populations are less likely to use mental health services. Digital mental health approaches have been shown to be effective in improving well-being for the general population. Thus, they may provide an effective and culturally appropriate strategy to bridge the treatment gap for these populations vulnerable to mental health risks.

Objective
This paper aims to provide the background and rationale for conducting a scoping review on digital mental health resources for asylum seekers, refugees, and immigrants. It also provides an outline of the methods and analyses, which will be used to answer the following questions. What are the available digital mental health resources for asylum seekers, refugees, and immigrants? Are they effective, feasible, appropriate, and accepted by the population? What are the knowledge gaps in the field?

Methods
The scoping review methodology will follow 5 phases: identifying the research question; identifying relevant studies; study selection; charting the data; and collating, summarizing, and reporting the results. Searches will be conducted in the following databases: EBSCOhost databases (CINAHL Plus with Full Text, MEDLINE with Full Text, APA PsycArticles, Psychology and Behavioral Sciences Collection, and APA PsycInfo), PubMed, and Scopus. Additionally, OpenGrey, Mednar, and Eldis will be searched for gray literature. All primary studies and gray literature in English concerning the use of information and communication technology to deliver services addressing mental health issues for asylum seekers, refugees, and immigrants will be included.

Results
This scoping review will provide an overview of the available digital mental health resources for asylum seekers, refugees, and immigrants and describe the implementation outcomes of feasibility, acceptability, and appropriateness of such approaches for those populations. Potential gaps in the field will also be identified.

Conclusions
As of February 2020, there were no scoping reviews, which assessed the effectiveness, feasibility, acceptability, and appropriateness of the available digital mental health resources for asylum seekers, refugees, and immigrants. This review will provide an extensive coverage on a promising and innovative intervention for such populations. It will give insight into the range of approaches, their effectiveness, and progress in their implementation. It will also provide valuable information for health practitioners, policy makers, and researchers working with the population.

International Registered Report Identifier (IRRID)
PRR1-10.2196/19031