bluuebunny/crossref_metadata_2025_split huggingface dataset:
Minimum 10-year follow-up of micro-arc oxidation coating on a cementless grit-blasted tapered-wedge stem of total hip arthroplasty: a multicentre study
Authors: Lim Young-Wook , Song Joo-Hyoun , Kwon Soon-Yong , Kim Yong-Sik , Byun Young-Seol , Lee Se-Won 
Published: November 2020

Background: Recently, a femoral stem treated with grit-blasting and micro-arc oxidation (MAO) coating has commercialised but medium-term follow-up studies are lacking. The aim of this study was to report the outcome of a grit-blasted and MAO-coated femoral component designed as a straight, double-wedged, tapered stem with a rectangular cross-section with minimum 10 years follow-up. Methods: Between March 2006 and December 2008, 309 primary total hip arthroplasties using a grit-blasted and MAO-coated femoral component were performed by 3 experienced hip surgeons in 3 hospitals. At minimum 10 years after index THA, 299 hips were living, 10 hips were deceased, and 65 hips were lost to follow-up or had a follow-up period <10 years. Finally, 234 hips were enrolled in this study. Results: Mean duration of clinical follow-up was 129.6 months. The mean Harris Hip Score was improved from 46.9 to 88.4 at the final follow-up. 4 hips were revised for 2 aseptic femoral loosening, 1 aseptic acetabular cup loosening and 1 late infection. 3 hips were revised for a periprosthetic femoral fracture requiring a femoral component revision. The average time to revision was 51.6 (range 0–148) months. Kaplan-Meier survivorship analysis with an end point of revision for any reason demonstrated a survival rate of 97.4% at 10 years. Survival was 98.7% with revision for aseptic loosening as the endpoint. Conclusions: The outcomes of a cementless grit-blasted and MAO-coated tapered-wedge stem of THA were excellent to satisfactory after a follow-up of at least 10 years.
COVID-19 and psychological distress in Norway: The role of trust in the healthcare system
Authors: Harris Samantha M. , Sandal Gro M. 
Published: November 2020

Aim: The study aims to examine groups at risk for psychological distress in connection with the COVID-19 outbreak, and the role of trust in the healthcare system as a possible moderator. Methods: Data were collected from a large sample of the Norwegian population ( n = 4008) through the Norwegian Citizen Panel (NCP). A linear regression was conducted to examine the effects of COVID-19 related risk factors on psychological distress, using the 10-item Hopkins Symptom Checklist (HSCL-10). Finally, we conducted a moderation analysis to examine the interaction of trust in the healthcare system and COVID-19 related risk factors. Results: A linear regression showed that female gender, younger age, lower level of education, being infected with COVID-19, being medically vulnerable, working in the healthcare system, being in voluntary quarantine and having an immigrant background predicted mean HSCL-10 scores. The moderation analysis revealed that people in the medically vulnerable group, those below 61, and those in quarantine reported higher psychological distress when they also had lower trust in the healthcare system. Conclusions: Findings indicate important groups to take into consideration in mental healthcare strategies and policies. However, most participants in the current study reported psychological distress levels that were below the clinical cut-off, suggesting that the majority may have coped relatively well in the early stages of the pandemic.
Accuracy of Flash Glucose Monitoring in Patients with Diabetes Mellitus on Hemodialysis and Its Relationship with Hydration Status
Authors: Genua Idoia , Sánchez-Hernandez Joan , Martínez Maria José , Pujol Isabel , Places Jaume , González Carmen , Martinez Esther , Díaz Juan Manuel , Chico Ana 
Published: November 2020

Background: The use of flash-glucose monitoring system FreeStyle Libre (FSL) has demonstrated benefits in metabolic control and quality of life in different populations with diabetes mellitus (DM), being funded in many countries. Due to this, DM subjects on hemodialysis (HD) are using FSL despite the accuracy in this population being unclear. The aims of the present study are to assess the accuracy of FSL in DM subjects on HD, its relationship to hydration status, and patient satisfaction. Material and Methods: A prospective study in 16 patients with DM in a chronic HD program was conducted. Interstitial glucose values from FSL during a 14-day period were compared to capillary glucose measurements obtained at the same time. Hydration status was measured via bioimpedance spectroscopy. Satisfaction with FSL was obtained from an ad hoc satisfaction questionnaire. Results: A total of 766 paired interstitial and capillary glucose levels were analyzed. A correlation coefficient of 0.936 was determined and a mean absolute relative difference (MARD) value of 23%, increasing to 29% during HD. MARD was not related to hydration status. Interstitial glucose values were lower during HD (100.1 ± 17 mg/dL) than within the 12 hours after (125.1 ± 39 mg/dL; P = .012) and the days without HD (134.2 ± 32 mg/dL; P = .001). Subjects showed high satisfaction with FSL. Conclusion: The accuracy of FSL in DM patients on HD is lower than in other populations, especially during HD sessions and is not related with the hydration status. Despite this, the degree of patient satisfaction with FSL is very high.
Utility of Percutaneous Ultrasonic Tenotomy for Tendinopathies: A Systematic Review
Authors: Vajapey Sravya , Ghenbot Sennay , Baria Michael R. , Magnussen Robert A. , Vasileff W. Kelton 
Published: November 2020

Context: Chronic tendinopathy is a challenging problem that can lead to significant disability and limitation in not only athletics but also activities of daily living. While there are many treatment techniques described for this overuse injury, no single modality has been proven superior to all others. With recent advances in medical technology, percutaneous ultrasonic tenotomy (PUT) for tendinosis has gained traction with promising results. Objective: To examine the data published on PUT for treatment of tendinopathy, analyze the outcomes of the procedure, including duration of pain relief and patient-reported outcomes, and assess the rate of complications associated with the procedure. Data Sources: PubMed, MEDLINE, EMBASE, and Google Scholar. Study Selection: The following combination of keywords was entered into the electronic search engines: ultrasonic tenotomy, ultrasound tenotomy, Tenex, and ultrasonic percutaneous tenotomy. The search results were screened for studies relevant to the topic. Only English-language studies were considered for inclusion. Studies consisting of level 4 evidence or higher and those involving human participants were included for more detailed evaluation. Level of Evidence: Level 4. Data Extraction: Articles meeting the inclusion criteria were sorted and reviewed. Type of tendinopathy studied, outcome measures, and complications were recorded. Both quantitative and qualitative analyses were performed on the data collected. Results: There were a total of 7 studies that met the inclusion criteria and quality measures—5 studies involving the treatment of elbow tendinopathy and 1 study each involving the management of Achilles tendinopathy and plantar fasciitis. PUT resulted in decreased pain/disability scores and improved functional outcome scores for chronic elbow tendinopathy and plantar fasciitis. Results for Achilles tendinopathy showed modest improvement in the short term, but long-term data are lacking. Conclusion: PUT is a minimally invasive treatment technique that can be considered in patients with tendinopathy refractory to conservative treatment measures. Further higher quality studies are necessary to accurately assess the comparative effectiveness of this treatment modality.
Do Sex-Specific Differences Exist in ACL Attachment Location? An MRI-Based 3-Dimensional Topographic Analysis
Authors: Dimitriou Dimitris , Wang Zhongzheng , Zou Diyang , Helmy Naeder , Tsai Tsung-Yuan 
Published: November 2020

Background: Female sex is an independent risk factor for an anterior cruciate ligament (ACL) injury, as the incidence of an ACL rupture is 4- to 6-fold higher in female athletes compared with their male counterparts. The ACL attachment location as a potential risk factor for the increased ACL rupture rate in women has never been reported in the literature. Purpose/Hypothesis The purpose of the present study was to investigate the 3-dimensional topographic anatomy of the ACL bundle attachment in female and male patients, with and without an ACL rupture, and identify potential sex-related differences. We hypothesized that the ACL attachment location would be significantly different between men and women, in both the intact- and ruptured-ACL states. Study Design: Cross-sectional study; Level of evidence, 3. Methods: Magnetic resonance images of the knee from 90 patients (55 men, 35 women) with a ruptured ACL and 90 matched controls (55 men, 35 women), who suffered a noncontact knee injury without ACL rupture, were used to create 3-dimensional models of the femur and tibia. The ACL bundles’ origin and insertion were outlined on each model, and their location was measured using an anatomical coordinate system. A 2-way analysis of variance was used to compare the ACL attachment location between male and female patients, with and without an ACL rupture. Results: No significant differences were found between female and male participants regarding ACL attachment location (femoral origin and tibial insertion). Patients with a ruptured ACL demonstrated a significantly different ACL origin compared with the participants with an intact ACL by an average difference of 8.9% more posterior ( P < .05) and 4.0% more proximal ( P < .05) in men and 13.0% more posterior ( P < .05) and 5.5% more proximal ( P < .05) to the flexion-extension axis of the knee in women. Conclusion: The ACL attachment location should not be considered a risk factor for the increased ACL rupture rates in female compared with male athletes. However, a more posterior and proximal location of the femoral ACL origin might be a predisposing factor to an ACL rupture regardless of sex.
Towards Selecting Effective Open Source Learning Management System/Software (OSLMS) for Higher Education Domain
Authors: Sk Md Ajimuddin , Jana Sibsankar , Khatun Samima 
Published: November 2020

We are in the age of information and communication technology (ICT), where the learning communities (i.e., learners, teachers, scholars, etc.), mainly in higher education domains, are more technology dependent. The stakeholders always handle advanced research, study and technologies, and therefore they need some authentic and updated information to satisfy their daily needs. They simply need some updating as well as real-time interactive digital learning platforms that can provide fast, authentic and updated information to satisfy their academic needs. This learning platform completely depends on the judicious designing methodologies, and this designing methodology totally depends on the reliable and effective digital learning tools or learning management system (LMS). The present study is an attempt to design the standard process towards selecting an authentic, reliable and effective open source learning management software/system (OSLMS) for the higher education platform. In this regard, the study has been designed in three layers for the selection of the popular and most downloaded OSLMS in the world. Later in the third layer, a comparative study of the final six OSLMSs has been conducted based on the features under various criteria and parameters to select the right one. Lastly, it is found that the Moodle fulfilled most of the criteria and is selected as the most authentic, interactive and trustworthy OSLMS for the higher education system.
IDH1 mutation contributes to myeloid dysplasia in mice by disturbing heme biosynthesis and erythropoiesis
Authors: Gu Yu , Yang Risheng , Yang Ying , Zhao Yuanlin , Wakeham Andrew , Li Wanda Y. , Tseng Alan , Leca Julie , Berger Thorsten , Saunders Mary , Fortin Jerome , Gao Xing , Yuan Yuan , Xiao Liming , Zhang Feng , Zhang Lijun , Gao Guangxun , Zhou Wenjing , Wang Zhe , Mak Tak W. , Ye Jing 
Published: November 2020

Abstract
Isocitrate dehydrogenase (IDH) mutations are common genetic alterations in myeloid disorders, including acute myeloid leukemia (AML) and myelodysplastic syndrome (MDS). Epigenetic changes, including abnormal histone and DNA methylation, have been implicated in the pathogenic build-up of hematopoietic progenitors, but it is still unclear whether and how IDH mutations themselves affect hematopoiesis. Here, we show that IDH1-mutant mice develop myeloid dysplasia in that these animals exhibit anemia, ineffective erythropoiesis, and increased immature progenitors and erythroblasts. In erythroid cells of these mice, D-2-hydroxyglutarate, an aberrant metabolite produced by the mutant IDH1 enzyme, inhibits oxoglutarate dehydrogenase activity and diminishes succinyl–coenzyme A (CoA) production. This succinyl-CoA deficiency attenuates heme biosynthesis in IDH1-mutant hematopoietic cells, thus blocking erythroid differentiation at the late erythroblast stage and the erythroid commitment of hematopoietic stem cells, while the exogenous succinyl-CoA or 5-ALA rescues erythropoiesis in IDH1-mutant erythroid cells. Heme deficiency also impairs heme oxygenase-1 expression, which reduces levels of important heme catabolites such as biliverdin and bilirubin. These deficits result in accumulation of excessive reactive oxygen species that induce the cell death of IDH1-mutant erythroid cells. Our results clearly show the essential role of IDH1 in normal erythropoiesis and describe how its mutation leads to myeloid disorders. These data thus have important implications for the devising of new treatments for IDH-mutant tumors.
A Review on Self-Recovery Regulation (SR) Technique for Unbalance Vibration of High-End Equipment
Authors: Pan Xin , Lu Jiaqiao , Huo Jiaji , Gao Jinji , Wu Haiqi 
Published: November 2020

AbstractThe high-end equipment represented by high-end machine tools and aero-engines is the core component of the national intelligent manufacturing plan, and the mass unbalance is the main reason for its excessive vibration, that seriously impacts the operation efficiency and running life of the equipment. In order to change the traditional way that the fault of equipment can only be repaired by human, the self-recovery mechanism of human and animal are given to the equipment in this paper, which forms the self-recovery regulation (SR) system for unbalance vibration of high-end equipment. The system can online generate the self-recovery force to restrain the unbalance vibration of the equipment in operation, which is an important direction for the development of the equipment to the advanced intelligent stage. Based on the basic principles of SR technique, the typical engineering application cases of this technique in the field of aeroengine and high-end machine tools are introduced, and four related studies promoting the development of this technique are summarized and analyzed in turn. It includes feature extraction, imbalance location, regulation method and balancing actuator. Self-recovery Regulation (SR) Technique is an important way to realize intelligent manufacturing and intelligent maintenance. Relevant research can lay a technical foundation for the development of high-end equipment with self-health function.
Unexpected diversity of CRISPR unveils some evolutionary patterns of repeated sequences in Mycobacterium tuberculosis
Authors: Refrégier Guislaine , Sola Christophe , Guyeux Christophe 
Published: November 2020

AbstractBackgroundDiversity of the CRISPR locus ofMycobacterium tuberculosiscomplex has been studied since 1997 for molecular epidemiology purposes. By targeting solely the 43 spacers present in the two first sequenced genomes (H37Rv and BCG), it gave a biased idea of CRISPR diversity and ignored diversity in the neighbouringcas-genes.ResultsWe set up tailored pipelines to explore the diversity of CRISPR-cas locus in Short Reads. We analyzed data from a representative set of 198 clinical isolates as evidenced by well-characterized SNPs.We found a relatively low diversity in terms of spacers: we recovered only the 68 spacers that had been described in 2000. We found no partial or global inversions in the sequences, letting always the Direct Variant Repeats (DVR) in the same order. In contrast, we found an unexpected diversity in the form of: SNPs in spacers and in Direct Repeats, duplications of various length, and insertions at various locations of the IS6110insertion sequence, as well as blocks of DVR deletions. The diversity was in part specific to lineages. When reconstructing evolutionary steps of the locus, we found no evidence for SNP reversal. DVR deletions were linked to recombination between IS6110insertions or between Direct Repeats.ConclusionThis work definitively shows that CRISPR locus ofM. tuberculosisdid not evolve by classical CRISPR adaptation (incorporation of new spacers) since the last most recent common ancestor of virulent lineages. The evolutionary mechanisms that we discovered could be involved in bacterial adaptation but in a way that remains to be identified.
Competitive mapping allows for the identification and exclusion of human DNA contamination in ancient faunal genomic datasets
Authors: Feuerborn Tatiana R. , Palkopoulou Eleftheria , van der Valk Tom , von Seth Johanna , Munters Arielle R. , Pečnerová Patrícia , Dehasque Marianne , Ureña Irene , Ersmark Erik , Lagerholm Vendela Kempe , Krzewińska Maja , Rodríguez-Varela Ricardo , Götherström Anders , Dalén Love , Díez-del-Molino David 
Published: November 2020

Abstract
Background
After over a decade of developments in field collection, laboratory methods and advances in high-throughput sequencing, contamination remains a key issue in ancient DNA research. Currently, human and microbial contaminant DNA still impose challenges on cost-effective sequencing and accurate interpretation of ancient DNA data.

Results
Here we investigate whether human contaminating DNA can be found in ancient faunal sequencing datasets. We identify variable levels of human contamination, which persists even after the sequence reads have been mapped to the faunal reference genomes. This contamination has the potential to affect a range of downstream analyses.

Conclusions
We propose a fast and simple method, based on competitive mapping, which allows identifying and removing human contamination from ancient faunal DNA datasets with limited losses of true ancient data. This method could represent an important tool for the ancient DNA field.
Virulence characterization and comparative genomics of Listeria monocytogenes sequence type 155 strains
Authors: Wagner Eva , Zaiser Andreas , Leitner Rebekka , Quijada Narciso M. , Pracser Nadja , Pietzka Ariane , Ruppitsch Werner , Schmitz-Esser Stephan , Wagner Martin , Rychli Kathrin 
Published: November 2020

AbstractBackgroundListeria(L.)monocytogenesstrains show a high diversity regarding stress tolerance and virulence potential. Genome studies have mainly focused on specific sequence types (STs) predominantly associated with either food or human listeriosis. This study focused on the prevalent ST155, showing equal distribution among clinical and food isolates. We evaluated the virulence potential of 20 ST155 strains and performed comparative genomic analysis of 130 ST155 strains isolated from food, food processing environments and human listeriosis cases in different countries and years.ResultsThe in vitro virulence assays using human intestinal epithelial Caco2 and hepatocytic HEPG2 cells showed an impaired virulence phenotype for six of the 20 selected ST155 strains. Genome analysis revealed no distinct clustering of strains from the same source category (food, food processing environment, and clinical isolates). All strains harbored an intactinlAandinlBlocus, except four strains, which had an internal deletion in theinlAgene. All strains harbored LIPI-1, butprfAwas present in a longer variant in six strains, all showing impaired virulence. The longer PrfA variant resulted in lower expression ofinlA,inlB,andprfA, and no expression ofhlyandactA.Regarding stress-related gene content, SSI-1 was present, whereasqacHwas absent in all strains. 34.6% of the strains harbored a plasmid. All but one ST155 plasmids showed high conservation and harboredcadA2,bcrABC, and a triphenylmethane reductase.ConclusionsThis study contributes to an enhanced understanding ofL. monocytogenesST155 strains, being equally distributed among isolates from humans, food, and food processing environments. The conservation of the present genetic traits and the absence of unique inherent genetic features makes these types of STs especially interesting since they are apparently equally adapted to the conditions in food processing environments, as well as in food as to the human host environment. However, a ST155-specific mutation resulting in a longer PrfA variant impaired the virulence potential of several ST155 strains.
The SAP function in pistil development was proved by two allelic mutations in Chinese cabbage (Brassica rapa L. ssp. pekinensis)
Authors: Huang Shengnan , Liu Wenjie , Xu Junjie , Liu Zhiyong , Li Chengyu , Feng Hui 
Published: November 2020

AbstractBackgroundPistil development is a complicated process in plants, and female sterile mutants are ideal material for screening and cloning pistil development-related genes. Using the female sterile mutant (fsm1),BraA04g009730.3Cwas previously predicted as a candidate mutant gene encoding the STERILE APETALA (SAP) transcriptional regulator. In the current study, a parallel female sterile mutant (fsm2) was derived from EMS mutagenesis of a Chinese cabbage DH line ‘FT’ seeds.ResultsBothfsm2andfsm1mutant phenotypes exhibited pistil abortion and smaller floral organs. Genetic analysis indicated that the phenotype of mutantfsm2was also controlled by a single recessive nuclear gene. Allelism testing showed that the mutatedfsm1andfsm2genes were allelic. A single-nucleotide mutation (G-to-A) in the first exon ofBraA04g009730.3Ccaused a missense mutation from GAA (glutamic acid) to GGA (glycine) in mutantfsm2plants. Both allelic mutations ofBraA04g009730.3Cinfsm1andfsm2conferred the similar pistil abortion phenotype, which verified theSAPfunction in pistil development. To probe the mechanism ofSAP-induced pistil abortion, we compared the mutantfsm1and wild-type ‘FT’ pistil transcriptomes. Among the 3855 differentially expressed genes obtained, 29 were related to ovule development and 16 were related to organ size.ConclusionOur study clarified the function ofBraA04g009730.3Cand revealed that it was responsible for ovule development and organ size. These results lay a foundation to elucidate the molecular mechanism of pistil development in Chinese cabbage.