bluuebunny/crossref_metadata_2025_split huggingface dataset:
Correlation between exposure to fine particulate matter and hypertensive disorders of pregnancy in Shanghai, China
Authors: Su Xiujuan , Zhao Yan , Yang Yingying , Hua Jing 
Published: February 2020

Abstract
Background Association between fine particulate matter (PM 2.5 ) and hypertensive disorders of pregnancy (HDP) is inconsistent and appears to change in each trimester. We aim to investigate the association of exposure to ambient PM 2.5 in early pregnancy with HDP. A retrospective cohort study was performed among 8,776 women with singleton pregnancy who attended the antenatal clinic before 20 gestational weeks in a tertiary women’s hospital during 2014 - 2015. Land use regression models were used to predict individual levels of PM 2.5 exposure. Results The average PM 2.5 concentration during the first 20 gestational weeks ranged from 28.6 to 74.8 μg m -3 [median, 51.4 μg m -3 ; interquartile range, 47.3 - 57.8 μg m -3 ]. A total of 440 (5.0%) women was diagnosed with HDP. The restricted cubic spline showed an exposure-response relationship between the PM 2.5 concentration and risk of HDP. We observed an association between PM 2.5 exposure during the first trimester with HDP (RR = 3.89 per 10 μg m -3 , 95% CI: 1.45 - 10.43), but not during the second trimester (RR = 0.71 per 10 μg m -3 , 95% CI: 0.40 - 1.27). Compared with their counterparts, nulliparous women who were exposed to high levels of PM 2.5 in the index pregnancy had a higher risk of developing HDP [the relative excess risk due to interaction was 0.92 (0.46 - 1.38)]. Conclusion Our findings suggest that PM 2.5 exposure during the first trimester is associated with the development of HDP, and the association is modified by parity.
Development of a tool for identifying and addressing prioritised determinants of quality improvement projects led by healthcare professionals: a mixed-methods study
Authors: Tuijl Anne van , Wollersheim Hub C. , Fluit Cornelia R.M.G. , Gurp Petra. J. van , Calsbeek Hiske 
Published: February 2020

Abstract
Background Several frameworks have been developed to identify essential determinants for healthcare improvement. These frameworks aim to be comprehensive, leading to the creation of long lists of non-prioritised determinants. Furthermore, most existing frameworks do not describe the methods or actions used to identify and address the determinants, limiting their practical value. The aim of this study is to describe the development of a tool to determine the prioritised facilitators and barriers for healthcare improvement projects led by healthcare professionals, supplemented with methods to identify and address each determinant. Methods A mixed-methods study design was used to develop the tool. First, a cross-sectional study was performed, using an online survey to ask healthcare professionals about the determinants they experienced during the performance of their quality improvement project. A priority score was calculated for every named determinant, and those with a priority score ≥ 20 were incorporated into the tool. Semi-structured interviews with implementation experts determined: 1) how healthcare professionals can be assisted to use our tool to analyse the determinants in their project and 2) how healthcare professionals can tackle the barriers or use the facilitators identified in their quality improvement project. Results The 25 healthcare professionals in this study experienced 64 facilitators and 66 barriers when performing their improvement projects. Of these, 12 facilitators and nine barriers were incorporated into the tool. Sufficient support from management of the department was identified as the most important facilitator, while having limited time to perform the project was considered the biggest barrier. The interviews with 16 experts in implementation science led to various inputs for better identifying and addressing each determinant. Important themes included maintaining adequate communication with stakeholders, keeping the project at a manageable size, learning by doing and being able to influence determinants. Conclusions This paper describes a tool for performing quality improvement projects in healthcare, with the barriers and facilitators that were considered most important by healthcare professionals. For each of these barriers and facilitators, suggestions on how to analyse and address the determinant are given. More research is needed to validate and develop the tool further.
The effectiveness of application of the standardized terminology for telephone follow-up model in discharge follow-up of patients with cancer pain.
Authors: PENG Xin , ZHANG Xing , REN Yi , XIONG juan li 
Published: February 2020

Abstract
Purpose This article aims to explore a standardized telephone follow-up model for patients with cancer pain, thus to promote standardized management of discharge follow-up of the patients with cancer pain.Method We have developed a flow chart of standard terminology for discharge follow-up of the patients with cancer pain. The 80 patients with cancer pain who have discharged from our hospital were divided into the control group (40 cases) and the observation group (40 cases), both groups were followed up by telephone within one week of discharge. The pain nurses have done the discharge follow up by telephone using the communication method of routine follow-up and the communication method of standardized terminology, self-monitoring, treatment compliance, adverse reaction management, self-management ability and satisfaction with telephone follow-up were compared between the two groups. Results The total scores of self-pain monitoring, treatment compliance, adverse reaction management and self-management ability in the observation group were higher than those in the control group (P<0.05). The satisfaction of the observation group was significantly higher than that of the control group (P<0.05).Conclusion The application of the standardized terminology telephone follow-up mode can promote effective communication between nurses and patients, standardize the process and content of telephone follow-up. It can effectively improve the effectiveness of telephone follow-up and the satisfaction of patients with nursing services, which is suitable for clinical reference.
Identification of a prognostic long non-coding RNA signature in breast cancer
Authors: Lan Gaochen , Yu Xiaoling , Zhao Yanna , Lan Jinjian , Li Wan , Gao Ruilan 
Published: February 2020

Abstract
Background: Breast cancer is the most common malignant disease among women. At present, more and more attention has been paid to long non-coding RNAs (lncRNAs) in the field of breast cancer research. We aimed to investigate the expression profiles of lncRNAs and construct a prognostic lncRNA for predicting the overall survival (OS) of breast cancer. Methods: The expression profiles of lncRNAs and clinical data with breast cancer were obtained from The Cancer Genome Atlas (TCGA). Differentially expressed lncRNAs were screened out by R package (limma). The survival probability was estimated by the Kaplan‑Meier Test. The Cox Regression Model was performed for univariate and multivariate analysis. The risk score (RS) was established on the basis of the lncRNAs’ expression level (exp) multiplied regression coefficient (β) from the multivariate cox regression analysis with the following formula: RS=exp a1 * β a1 + exp a2 * β a2 +……+ exp an * β an . Functional enrichment analysis was performed by Metascape. Results: A total of 3404 differentially expressed lncRNAs were identified. Among them, CYTOR , MIR4458HG and MAPT-AS1 were significantly associated with the survival of breast cancer. Finally, The RS could predict OS of breast cancer (RS=exp CYTOR * β CYTOR + exp MIR4458HG * β MIR4458HG + exp MAPT-AS1 * β MAPT-AS1 ). Moreover, it was confirmed that the three-lncRNA signature could be an independent prognostic biomarker for breast cancer (HR=3.040, P=0.000). Conclusions: This study established a three-lncRNA signature, which might be a novel prognostic biomarker for breast cancer.
Clinicopathological and prognostic significance of maspin expression in resected non-small cell lung cancer: a meta-analysis
Authors: Wang Yan , Dong Yingxian , Wang Yanwen , Li Jialong , Che Guowei 
Published: February 2020

Abstract
Objective To explore the association of maspin expression with clinical pathological parameters and prognosis in non-small cell lung cancer (NSCLC) who received the surgical therapy. Methods The EMBASE, Web of Science and PubMed were searched to identify eligible studies to 3 June, 2019. The correlation of maspin expression with clinicopathological characteristics and survival of resected NSCLC patients was assessed by the combined relative risk (RR) and hazard ratio (HR) with corresponding 95% confidence interval (CI), respectively. All statistical analyses were performed via the Stata 12.0 software. Results A total of 12 articles involving 1771 patients were included. The results manifested that maspin was more prevalent in lung squamous cell carcinoma (SCC) (RR=0.36, 95% CI: 0.22-0.60, P <0.001) and significantly associated with P53 expression (RR=1.50, 95% CI: 1.00-2.24, P =0.048), while no significant correlation of maspin with other clinicopathological parameters such as the gender, age, tumor size, lymph node metastasis, tumor node metastasis (TNM) stage or differentiation status was observed. As for the prognosis representing as overall survival (OS), disease-free survival (DFS) and cancer-specific survival (CSS), only significant association between maspin and CSS (HR=1.58, 95% CI: 1.16-2.15, P =0.003) was revealed. However, the subgroup analysis for OS based on the histology demonstrated that maspin expression was an unfavourable and favourable prognostic indicator in lung adenocarcinoma (HR=3.36, 95% CI: 1.44-7.87, P =0.005) and SCC (HR=0.44, 95% CI: 0.27-0.71, P =0.001), respectively. Conclusion Maspin expression was correlated with histology type and P53 expression, but no certain association of maspin with other clinicopathological characteristics or prognosis was observed in resected NSCLC. More well-designed prospective researches with big samples are still needed to further assess the clinicopathological and prognostic significance of maspin in NSCLC patients undergoing surgical resection.
Knockdown of LncRNA SNHG14 regulates proliferation and apoptosis via activating miR-204-5p/HMGA1 axis in retinoblastoma
Authors: Chen Xuedong , Tang Xianling , Zhao Shiyong , Yu Yongbin , Li Qingjun , Xu Caicai , Ge Hongyan 
Published: February 2020

Abstract
Background: Retinoblastoma (RB) is an aggressive intraocular malignancy of infant and childhood, which seriously endangers the vision and life of children. Long non-coding RNA Small Nucleolar RNA Host Gene 14 (lncRNA SNHG14) as a novel oncogene is involved in the control of cancer cell progression. However, the effects and molecular mechanism of SNHG14 on retinoblastoma remain confusing.Methods: Levels of SNHG14, high mobility group protein A1 (HMGA1) mRNA and microRNA (miR)-204-5p were detected by quantitative real-time polymerase chain reaction. Cell proliferation and apoptosis were measured by CKK-8 assay or flow cytometry, respectively. Western blot was used to detect HMGA1 and apoptosis-related protein. The interaction between miR-204-5p and SNHG14 or HMGA1 was explored by luciferase reporter assay. Murine xenograft model was established using Y79 cells stably transfected with sh-SNHG14.Results: SNHG14 and HMGA1 were up-regulated in retinoblastoma tissues and cell lines, and knockdown of SNHG14 or HMGA1 suppressed cell proliferation and induced cell apoptosis in retinoblastoma. MiR-204-5p was confirmed to directly bind to SNHG14 and HMGA1, SNHG14 positively regulated HMGA1 expression via miR-204-5p. Importantly, the anti-tumor effects mediated by SNHG14 knockdown could be reversed by miR-204-5p inhibition or HMGA1 overexpression in retinoblastoma cells. Furthermore, xenograft model showed SNHG14 silence impeded tumor growth in vivo.Conclusion: Knockdown of SNHG14 suppressed retinoblastoma progression by regulating miR-204-5p/HMGA1 axis, revealing a potential target to develop appropriate treatment strategies for retinoblastoma.
Measurement of central venous pressure is associated with better outcomes in septic patients: a retrospective observational study
Authors: Chen Hui , Zhu Zhu , Zhao Chenyan , Guo Yanxia , Chen Dongyu , Wei Yao , Jin Jun 
Published: February 2020

Abstract
Purpose: With the proper insights, measurement of central venous pressure (CVP) can be a useful clinical aid. However, the formal utility of CVP measurement on mortality in septic patients has never been proved.Methods: The Medical Information Mart for Intensive Care III (MIMIC-III) was applied to identify septic patients who had and did not have CVP measured. The primary outcome was 28-day mortality. The statistical approaches including multivariate regression, propensity score matching (PSM) and an inverse probability of treatment weighing (IPTW) and causal mediation analysis (CMA) were utilized to elucidate the relationship between CVP measurement and 28-day mortality.Results: A total of 10275 patients were included in our study, of which 4516 patients (44%) had CVP measured within 24 h after ICU admission. A significant beneficial effect of CVP measurement in terms of 28-day mortality was observed (OR 0.60 (95% CI 0.51–0.70; p<0.001)). Patients in CVP group received more fluid on day 1, had a shorter duration of mechanical ventilation and vasopressor use, and the reduction of serum lactate was higher than that in the no CVP group. The mediation effect of serum lactate reduction was significant for the whole cohort (p=0.04 for average causal mediation effect (ACME)) and patients in the CVP group with an initial CVP level below 8mmHg (p=0.04 for ACME).Conclusion: CVP measurement is associated with a lower risk-adjusted 28-day mortality among patients with sepsis, which is proportionally mediated through serum lactate reduction.
Inverse Association of Serum Fibroblast Growth Factor 19 and Atherogenic Index of Plasma in Type 2 Diabetic Patients
Authors: Liu Wangshu , Xu Tianli , Tang Mengjie , Wang Xue-Qin , Su Jianbin , Xu Feng , Zhang Dongmei , Zhu Qing , Cao Jie , Wang Hong 
Published: February 2020

Abstract
BackgroundWe aimed to explore the relationship between serum fibroblast growth factor 19 (FGF19) and the atherogenic index of plasma (AIP) in type 2 diabetic patients.MethodsSerum FGF19 levels and lipid profiles were measured in 200 patients with type 2 diabetes (T2D). The levels of serum FGF19 were measured by ELISA. Lipid profiles were measured by enzymatic analysis. AIP and NAFLD fibrosis scores were calculated.ResultsT2D patients showed a significant decreasing trend of FGF19 concentrations depending on the tertiles of AIP (p for trend < 0.05). Simultaneously, the AIP level was closely related to the serum FGF19 level (p < 0.05). Furthermore, after adjusting for age, sex, duration, BMI, hypertension, and diabetic treatment, the correlation was still significant (p < 0.01), and it remained significant even after further adjusting for non-alcoholic fatty liver disease (NAFLD) and NAFLD fibrosis score (NFS) (p < 0.01). However, when stratified by BMI, AIP was positively correlated with FGF19 in normal-weight and overweight T2D patients but not in obese T2D subjects. After adjusting for sex, age, BMI, duration, hypertension, HbA1c, 2hPG, HOMA-IR, AIP, antidiabetic treatments, NAFLD and NFS via multiple stepwise linear regression, AIP was an independent factor affecting serum FGF19 concentrations (SE = 0.238, β = -0.290, p < 0.01).ConclusionsSerum FGF19 levels might be a good predictor for atherosclerosis and cardiovascular disease in T2D patients, especially among non-obese patients; serum FGF19 levels were significantly inversely associated with AIP.
How do older women perceive their safety in urban outdoor environments?
Authors: Lak Azadeh , Aghamolaei Reihaneh , Myint Phyo K 
Published: February 2020

Abstract
Backgrounds According to the elders' health as the main priority of Ageing in place, recent research focuses on better understanding of the elders' needs of perceived safety especially the elder women as the vulnerable group in urban open environments. The aim of this study is to explore the perception of elder women about safety and its related features in outdoor spaces according to promoting active ageing. Methodology We carried out a mixed-method study in which the data collection was conducted through content analysis. Semi-structured interviews were held with 54 older women (mean age 78.40 years). The participants were recruited in open spaces of Tehran applying the purposeful sampling and continued saturation during summer and fall of 2018. All interviews were recorded and transcribed verbatim. The qualitative content analysis was used to extract sub-themes and main themes. In order to prioritize the extracted concepts, analytic hierarchy process was applied in quantitative analysis (AHP) in quantitative phase. Findings According to findings, the perception of safety in female elders can be described in three main themes consisting of psychological, functional and environmental safety, in that order of priority. Psychological safety includes fears of falling, getting lost, social limitation, anxiety, and social support or capital while functional safety consists of fears of public transportation, walkability, and physical activity. Finally, environmental safety comprises of fear of road traffic accidence, criminals, upkeep, incivility, and nuisance. Conclusion Our findings provide both personal and environmental features that can be targeted and requires implementation from policymakers and urban developers to meet the needs of growing older vulnerable population to achieve global active ageing goals.
Neurobiological Alterations Of The Substantia Nigra In Sudden Infant Death Syndrome
Authors: Lavezzi Anna , Mehboob Riffat , Alfonsi Graziella , Ferrero Stefano 
Published: February 2020

Abstract
Background - The purpose of this study was to research possible morphological and functional alterations of the substantia nigra in SIDS. Methods - Brainstems of 46 victims of sudden infant death, aged from 1 to 7 months, were investigated. Twenty-six of these cases were diagnosed as SIDS, due to the lack of any pathological finding, while the remaining 20 cases, in which the cause of death was determined at autopsy, served as controls. Maternal smoking was reported in 10% of controls and 77% of SIDS. Histopathological examination of the substantia nigra was done on midbrain 5-µm-thick sections stained with hematoxylin-eosin and Klüver-Barrera. Densitometry, immunohistochemistry and histochemistry were applied to highlight the neuronal concentration, the expression of tyrosine hydroxylase and neuromelanin, respectively. Results - Hypoplasia of the pars compacta of the substantia nigra was observed in SIDS but not in controls. Tyrosine hydroxylase expression in the substantia nigra was significantly higher in controls than in SIDS. Neuromelanin was observed as dark granules only in 4 infants of the control group but never in SIDS. A significant correlation was found between hypoplasia/low neuronal density, low tyrosine hydroxylase expression in the substantia nigra pars compacta and maternal smoking. Conclusion - The substantia nigra pars compacta, that is the main dopamine center in the brain, is involved in the control of many functions, including the sleep-arousal phase, and hence, its structural and/or functional alterations may explain the pathogenesis of SIDS, that often occurs during sleep. This study also indicates that maternal smoking can may have strongly influenced the SN altered development. Trial registration – not applicable for this study
Synergistic effect of combined GDNF and Amodiaquine attenuates behavioral deficits and protect dopaminergic neurons of Parkinson’s disease animal model through tyrosine kinase Ret receptor
Authors: Kambey Piniel , Gao Dianshuai 
Published: February 2020

Abstract
Parkinson’s disease (PD) is one among the most leading neurodegenerative disease after Alzheimer’s disease, with a prevalence of approximately 0.5–1% among those 65–69 years of age. Efforts to vitiate this disease are ongoing, and several treatment modes such as Glial cell line-derived neurotrophic factor (GDNF) have been in place since 1993. Glial cell line derived neurotrophic factor (GDNF) protects, regenerates, and improves the metabolism of substantia nigra pars compacta neurons (SNpc), and it increases the high-affinity dopamine uptake. It has been recently reported that amodiquine could attenuates the behavioral deficits of an animal model of Parkinson’s disease, nevertheless it mechanism is obscure. We sought to demonstrate the mechanism of neuro-protection effect of amodiquine and ascertain its corroborative effect when used togather with GDNF. We show herein that combined therapy (GDNF and amodiaquine) ameliorated behavioral deficits of PD animal models as compared to single-factor treatment. TH positive neurons increased significantly upon combined therapy treatment, and besides, GDNF and amodiaquine interact functionally to protect dopaminergic neurons through the PIK-3/Akt pathway. We also found that combined therapy (GDNF and amodiaquine) mediates its action through a distinct trans-membrane tyrosine kinase Ret receptor by amplifying its effect. Slight elevated aspartate aminotransferase (AST) were noticed in amodiaquine treated groups, alarming the bio-utility. These findings collectively suggest an interplay between GDNF and amodiaquine and co-express to exert neuronal protection hence a promising approach in PD therapy. Despite its undisputed effect on neuro-protection, we report that amodiaquine may not be safe, particularly in translation to human beings' trial settings.
Inhibition of mammalian target of rapamycin (mTOR) improves neurobehavioral deficit and modulates inflammatory response after traumatic brain injury
Authors: Paterniti Irene , Campolo Michela , Casili Giovanna , Lanza Marika , Filippone Alessia , Cordaro Marika , Ardizzone Alessio , Scuderi Sarah , Cuzzocrea Salvatore , Esposito Emanuela 
Published: February 2020

Abstract
Traumatic brain injury (TBI) induce primary and secondary damage on endothelium and brain parenchyma, leading neurons die rapidly by necrosis. The mammalian target of rapamycin signalling pathway (mTOR) mediates many aspects of cell growth and regeneration and is up-regulated after moderate to severe traumatic brain injury (TBI). The significance of this increased signalling event for recovery of brain function is presently unclear, here we used two different selective inhibitors of mTOR activity to explore the functional role of mTOR inhibition in an validated model of TBI, the controlled cortical impact injury (CCI). We treated animals withKU0063794, a dual mTORC1 and mTORC2 inhibitor, and with rapamycin a well-known inhibitor of mTOR, 1 and 4 hours after TBI. Our results demonstrated that mTOR inhibitors, especially KU0063794, significantly improve motor and cognitive recovery after TBI as well as reduce lesion volumes. Moreover we observed that mTOR inhibitors treatment ameliorate the neuroinflammation associated to TBI and showed that this acute treatment significantly diminished the extent of neuronal death, astrogliosis and apoptotic process after trauma. Our findings suggest that the neuronal mTORC1/2 activity after TBI is deleterious to brain function and acute intervention with selective mTORC1/2 inhibitor may represent an effective therapeutic strategy to improve recovery after brain trauma.