bluuebunny/crossref_metadata_2025_split huggingface dataset:
Permafrost degradation in the Western Russian Arctic
Authors: Vasiliev Alexander A , Drozdov Dmitry S , Gravis Andrey G , Malkova Galina V , Nyland Kelsey E , Streletskiy Dmitry A 
Published: March 2020

Abstract
The Global Climate Observing System and Global Terrestrial Observing Network have identified permafrost as an ‘Essential Climate Variable,’ for which ground temperature and active layer dynamics are key variables. This work presents long-term climate, and permafrost monitoring data at seven sites representative of diverse climatic and environmental conditions in the western Russian Arctic. The region of interest is experiencing some of the highest rates of permafrost degradation globally. Since 1970, mean annual air temperatures and precipitation have increased at rates from 0.05 to 0.07 °C yr−1 and 1 to 3 mm yr−1 respectively. In response to changing climate, all seven sites examined show evidence of rapid permafrost degradation. Mean annual ground temperatures increases from 0.03 to 0.06 °C yr−1 at 10–12 m depth were observed in continuous permafrost zone. The permafrost table at all sites has lowered, up to 8 m in the discontinuous permafrost zone. Three stages of permafrost degradation are characterized for the western Russian Arctic based on the observations reported.
Seasonal Abundance and Diversity of Fruit Flies (Diptera: Tephritidae) in Three Types of Plant Formations in Western Burkina Faso, West Africa
Authors: Zida Issaka , Nacro Souleymane , Dabiré Rémy , Somda Irénée 
Published: March 2020

Abstract
Fruit flies are significant insect pests, worldwide. Tephritid species diversity and their seasonal abundance were investigated over 2 yr (May 2017 to May 2019) in Western Burkina Faso. A mass trapping experiment consisting of 288 Tephri Trap types, operating with four types of parapheromones comprising methyl eugenol, terpinyl acetate, trimedlure, and cue lure and an insecticide (Dichlorvos), was used for attracting and killing insects. Plant formations including natural fallows, mango orchards, and agroforestry parks in each of the six study sites were selected for data collection. Twenty-nine tephritid species belonging to 10 genera were identified. Fourteen fruit fly species were identified for the first time in Burkina Faso. The genera Ceratitis MacLeay (Diptera : Tephritidae) and Dacus Fabricius (Diptera: Tephritidae) with, respectively, 14 and 7 species recorded were the most represented. The dominant species caught was the invasive Bactrocera dorsalis Hendel (Diptera: Tephritidae) followed by Ceratitis cosyra Walker (Diptera: Tephritidae) and Ceratitis silvestrii Bezzi (Diptera: Tephritidae). The fruit fly population density was very high during the rainy season, with peaks occurring in June or July. The fruit fly species were generally more abundant during the hot and rainy seasons than during the cold and dry seasons. The highest diversity was recorded in natural fallows, as compared with the mango orchards and agroforestry parks. Tephritid species found refuge in the mango orchards during the dry and cold periods. The results of that investigation may be used for developing a sustainable pest management strategy for commercial orchards.
The Malaria-High Blood Pressure Hypothesis: Revisited
Authors: Nwokocha Chukwuemeka R , Bafor Enitome E , Ajayi Olutayo I , Ebeigbe Anthony B 
Published: March 2020

AbstractMalaria etiologies with pathophysiological similarities to hypertension currently constitute a major subject of research. The malaria-high blood pressure hypothesis is strongly supported by observations of the increasing incidence of hypertension in malaria-endemic, low- and middle-income countries with poor socioeconomic conditions, particularly in sub-Saharan African countries. Malnutrition and low birth weight with persistent symptomatic malaria presentations in pregnancy correlate strongly with the development of preeclampsia, gestational hypertension and subsequent hypertension in adult life. Evidence suggest that the link between malaria infection and high blood pressure involves interactions between malaria parasites and erythrocytes, the inflammatory process, effects of the infection during pregnancy; effects on renal and vascular functions as well as effects in sickle cell disease. Possible mechanisms which provide justification for the malaria-high blood pressure hypothesis include the following: endothelial dysfunction (reduced nitric oxide (NO) levels), impaired release of local neurotransmitters and cytokines, decrease in vascular smooth muscle cell viability and/or alterations in cellular calcium signaling leading to enhanced vascular reactivity, remodeling, and cardiomyopathies, deranged homeostasis through dehydration, elevated intracellular mediators and proinflammatory cytokine responses, possible genetic regulations, activation of the renin–angiotensin–aldosterone system mechanisms and renal derangements, severe anemia and hemolysis, renal failure, and end organ damage. Two key mediators of the malaria-high blood pressure association are: endothelial dysfunction (reduced NO) and increased angiotensin-converting enzyme activity/angiotensin II levels. Sickle cell disease is associated with protection against malaria infection and reduced blood pressure. In this review, we present the state of knowledge about the malaria-blood pressure hypothesis and suggest insights for future studies.
Reforming Criminal Justice in the Ottoman Empire: Police, Courts and Prisons in Rusçuk, 1839-1864
Authors: Celik Mehmet 
Published: March 2020

Abstract
This case study explores the experimentation phase of the Ottoman Tanzimat reforms on the criminal justice system in the city of Rusçuk from 1839-64. In particular, it investigates crime and punishment by focusing on police, courts, and prisons and how these institutions responded to reform efforts in Rusçuk, which became the capital of the Danube Province in 1864. It shows that the Ottoman government established new police forces (zaptiye) and modernised prisons in the city in 1846 immediately after their introduction in the imperial capital of Istanbul. At the same time, the government bestowed extensive judicial authority on the meclis-i kebir (a secular administrative council in the provinces), and to a lesser extent on the meclis-i muvakkat (temporary council), over criminal cases. While the Sharia courts continued to enforce Islamic criminal law, the meclis-i kebir took charge of enforcing the new penal codes of 1840, 1851, and 1858, and served as a precursor first to the secular courts of the 1864 Provincial Reform and then to the more centralised and standardised nizamiye courts of the 1870s. This study also analyses the types and frequency of crimes and the penalties they received. Based on Rusçuk’s prison registers, which contain the cases tried by the meclis-i kebir and meclis-i muvakkat, and the records of the meclis-i vala (Supreme Court) in Istanbul, it argues that the crime rate in Rusçuk was much higher than the one represented in the Sharia court’s records.
Outcomes With Severe Blastomycosis and Respiratory Failure in the United States
Authors: Rush Barret , Lother Sylvain , Paunovic Bojan , Mooney Owen , Kumar Anand 
Published: March 2020

Abstract

Background
Outcomes of patients with severe pulmonary blastomycosis requiring mechanical ventilation (MV) are not well understood in the modern era. Limited historical case series reported 50–90% mortality in patients with acute respiratory distress syndrome caused by blastomycosis. The objective of this large retrospective cohort study was to describe the risk factors and outcomes of patients with severe pulmonary blastomycosis.

Methods
We performed a retrospective cohort analysis utilizing the Nationwide Inpatient Sample from 2006–2014. Patients aged &gt;18 years with a diagnosis of blastomycosis who received MV were included.

Results
There were 1848 patients with a diagnosis of blastomycosis included in the study. Of these, 219 (11.9%) underwent MV with a mortality rate of 39.7% compared with 2.5% in patients not requiring ventilatory support (P &lt; .01). The median (IQR) time to death for patients requiring MV was 12 (8–16) days. The median length of hospital stay for survivors of MV was 22 (14–37) days. The rate of MV was higher for patients treated in teaching hospitals (63.4% vs 57.2%, P = .05) and lower for those receiving care at a rural hospital (12.3% vs 17.2%, P = .04). In a multivariate model, female gender was associated with increased risk of mortality (OR, 1.84; 95% CI, 1.06–3.20; P = .03) as was increasing patient age (10-year age increase OR, 1.64; 95% CI, 1.33–2.02; P &lt; .01).

Conclusions
In the largest published cohort of patients with blastomycosis, mortality for patients on MV is high at ~40%, 16-fold higher than those without MV.
Human Immunodeficiency Virus Training Pathways in Residency: A National Survey of Curricula and Outcomes
Authors: Budak Jehan Z , Sears David A , Wood Brian R , Spach David H , Armstrong Wendy S , Dhanireddy Shireesha , Teherani Arianne , Schwartz Brian S 
Published: March 2020

Abstract

Background
The Ending the HIV Epidemic initiative, which aims to decrease the annual incidence of HIV infections in the United States (US) by 90% over the next decade, will require growth of a limited HIV provider workforce. Existing HIV training pathways within Family Medicine (FM) and Internal Medicine (IM) residency programs may address the shortage of HIV medical providers, but their curricula and outcomes have not previously been assessed.

Methods
We identified HIV residency pathways via literature review, Internet search, and snowball sampling and designed a cross-sectional study of existing HIV pathways in the US. This survey of pathway directors included 33 quantitative items regarding pathway organization, curricular content, graduate outcomes, and challenges. We used descriptive statistics to summarize responses.

Results
Twenty-five residency programs with dedicated HIV pathways in the US were identified (14 FM and 11 IM), with most located in the West and Northeast. All 25 (100%) pathway directors completed the survey. Since 2006, a total of 228 residents (77 FM and 151 IM) have graduated from these HIV pathways. Ninety (39%) of 228 pathway graduates provide primary care to persons with HIV (PWH).

Conclusions
HIV pathways are effective in graduating providers who can care for PWH, but generally are not located in nor do graduates practice in the geographic areas of highest need. Our findings can inform quality improvement for existing programs, development of new pathways, and workforce development strategies. Specifically, expanding pathways in regions of greatest need and incentivizing pathway graduates to work in these regions could augment the HIV workforce.
‘Codification by Interpretation’: The International Law Commission as an Interpreter of International Law
Authors: Azaria Danae 
Published: March 2020

Abstract
This article argues that the International Law Commission (ILC) interprets international law. In recent years, in documents intended to remain non-binding, the Commission has made interpretative pronouncements about a treaty in force, the Vienna Convention on the Law of Treaties, and customary international law reflected therein. This development is called the ‘codification by interpretation’ paradigm in this article. This article argues that interpretation falls within the ILC’s function, and it analyses the effects of the Commission’s interpretative pronouncements. It explains that the ILC’s interpretative pronouncements are not per se binding or authentic. However, they may trigger an interpretative dialogue with states. The ILC’s interpretative pronouncements may constitute a focal point for coordination among states, a subsidiary means for determining rules of law and a supplementary means of (treaty) interpretation. The aim of the ILC’s ‘codification-by-interpretation’ paradigm in the four topics considered in this article is to introduce clarity and predictability into secondary rules on the law of treaties, thus ensuring the clarity and predictability of primary treaty rules across all fields of international law. The ILC endeavours to convince states to use international law as a medium by which they regulate their affairs.
The status of preimplantation genetic testing in the UK and USA
Authors: Theobald Rachel , SenGupta Sioban , Harper Joyce 
Published: March 2020

Abstract

STUDY QUESTION
Has the number of preimplantation genetic testing (PGT) cycles in the UK and USA changed between 2014 and 2016?

SUMMARY ANSWER
From 2014 to 2016, the number of PGT cycles in the UK has remained the same at just under 2% but in the USA has increased from 13% to 27%.

WHAT IS KNOWN ALREADY
PGT was introduced as a treatment option for couples at risk of transmitting a known genetic or chromosomal abnormality to their child. This technology has also been applied as an embryo selection tool in the hope of increasing live birth rates per transfer. ART cycles are monitored in the UK by the Human Fertilisation and Embryology Authority (HFEA) and in the USA by the Society for Assisted Reproductive Technology (SART). Globally, data are monitored via the ESHRE PGT Consortium.

STUDY DESIGN, SIZE, DURATION
This cross-sectional study used the HFEA and SART databases to analyse PGT cycle data and make comparisons with IVF data to examine the success of and changes in patient treatment pathways. Both data sets were analysed from 2014 to 2016. The UK data included 3385 PGT cycles and the USA data included 94 935 PGT cycles.

PARTICIPANTS/MATERIALS, SETTING, METHODS
Following an extensive review of both databases, filters were applied to analyse the data. An assessment of limitations of each database was also undertaken, taking into account data collection by the ESHRE PGT Consortium. In the UK and USA, the publicly available information from these datasets cannot be separated into different indications.

MAIN RESULTS AND THE ROLE OF CHANCE
The proportion of PGT cycles as a total of ART procedures has remained the same in the UK but increased annually in the USA from 13% to 27%. Between 2014 and 2016 inclusive, 3385 PGT cycles have been performed in the UK, resulting in 1074 PGT babies being born. In the USA 94 935 PGT cycles have been performed, resulting in 26 822 babies being born. This gave a success rate per egg collection for PGT of 32% for the UK and 28% for the USA. Analysis of the data by maternal age shows very different patient populations between the UK and USA. These differences may be related to the way PGT is funded in the UK and USA and the lack of HFEA support for PGT for aneuploidy.

LIMITATIONS, REASONS FOR CAUTION
Data reported by the HFEA and SART have different limitations. As undertaken by the ESHRE PGT Consortium, both data sets should separate PGT data by indication. Although the HFEA collects data from all IVF clinics in the UK, SART data only represent 83% of clinics in the USA.

WIDER IMPLICATIONS OF THE FINDINGS
Worldwide, a consistent reporting scheme is required in which success rates can convey the effectiveness of PGT approaches for all indications.

STUDY FUNDING/COMPETING INTEREST(S)
No specific funding was obtained and there are no competing interests to declare that are directly related to this project. Joyce Harper is the director of the Embryology and PGD Academy, which offers education in these fields.
Acute type A aortic dissection in non-agenarians: to cut or not
Authors: Hattori Shigeru , Noguchi Kenichiro , Gunji Yusuke , Nagatsuka Motoki , Katayama Ikuo 
Published: March 2020

Abstract

OBJECTIVES
Surgery for acute type A aortic dissection (type AAD) in non-agenarians is usually contraindicated due to advanced age. The aim of this study was to assess and compare outcomes after surgical or conservative treatment for acute type AAD in non-agenarians by evaluating frailty.

METHODS
Between October 2012 and September 2018, 273 patients underwent open repair for type AAD at the Shonan Kamakura General Hospital and the Shonan Fujisawa Tokushukai Hospital, and here, we retrospectively reviewed the case reports of 10 surgically treated non-agenarians and 15 conservatively treated non-agenarians. Exclusion criteria for surgery were the patient’s refusal of surgery, severe dementia and coma. In patients considered to be at a high risk, our judgements were based on the results of comprehensive evaluation.

RESULTS
Both in-hospital mortality and 30-day mortality in the surgical group were zero, while in-hospital mortality in conservatively treated non-agenarians was 73.3%. Importantly, 1-year survival in the surgical group and conservative group was 90% and 25%, respectively. The 5-year survival in the surgical group and conservative group was 49.2% and 25%, respectively (log-rank test, P = 0.0105). Four of 6 patients with preoperative clinical frailty scores not higher than 4 were still alive at 1 year with the same level of preoperative frailty.

CONCLUSIONS
Surgery for acute type AAD in non-agenarians can be performed with acceptable outcomes in carefully selected patients, particularly in those with preoperative clinical frailty scores not higher than 4.
Adapting Labour Law to Complex Organisational Settings of the Enterprise: Why Rethinking the Concept of the Employer is Not Enough
Authors: Gaudio Giovanni 
Published: March 2020

Abstract
Labour law struggles to deal with the vertical disintegration of the enterprise, a phenomenon that questions the traditional bilateral and contractual analysis of the employment relationship and the unitary concept of the employer. Multiple employer patterns have been proposed by the Italian and English scholarship to try to sidestep the current impasse. However, these seem to be inconsistent with the existing legal framework and, in addition, it is debatable that they can be always instrumental in addressing the issues arising from the vertical disintegration of the enterprise. Nevertheless, an alternative and more nuanced analytical path can be followed. Labour law mostly takes the view that the employer is the contractual counterparty to the employee. Yet it also recognises that other entities can assume certain responsibilities of the employer in certain specific regulatory domains, where legislators recur to particular regulatory strategies often independent of a contractual analysis of the employment relationship. This article argues that the law takes this step not because these other legal entities are functionally akin to employers, but precisely in spite of the differences between them and the employer form. Rather than seeking to redefine the concept of employer, a better understanding of the subject must recognise that employment law consists of a kaleidoscopic blend of different regulatory domains, characterised by a range of different purposes, the achievement of which requires the adoption of different and even non-contractual normative tools. Adopting a variable geometry approach to frame the scope of labour laws would constitute a better analytical response to potentially restore the coherence and completeness of the scope of employment protective norms.
Primary and Secondary Dengue Virus Infections Elicit Similar Memory B-Cell Responses, but Breadth to Other Serotypes and Cross-Reactivity to Zika Virus Is Higher in Secondary Dengue
Authors: Andrade Paulina , Narvekar Parnal , Montoya Magelda , Michlmayr Daniela , Balmaseda Angel , Coloma Josefina , Harris Eva 
Published: March 2020

Abstract

Background
The 4 antigenically distinct serotypes of dengue virus (DENV) share extensive homology with each other and with the closely related Zika flavivirus (ZIKV). The development of polyclonal memory B cells (MBCs) to the 4 DENV serotypes and ZIKV during DENV infection is not fully understood.

Methods
In this study, we analyzed polyclonal MBCs at the single-cell level from peripheral blood mononuclear cells collected ~2 weeks or 6–7 months postprimary or postsecondary DENV infection from a pediatric hospital-based study in Nicaragua using a Multi-Color FluoroSpot assay.

Results
Dengue virus elicits robust type-specific and cross-reactive MBC responses after primary and secondary DENV infection, with a significantly higher cross-reactive response in both. Reactivity to the infecting serotype dominated the total MBC response. Although the frequency and proportion of type-specific and cross-reactive MBCs were comparable between primary and secondary DENV infections, within the cross-reactive response, the breadth of MBC responses against different serotypes was greater after secondary DENV infection. Dengue virus infection also induced cross-reactive MBC responses recognizing ZIKV, particularly after secondary DENV infection.

Conclusions
Overall, our study sheds light on the polyclonal MBC response to DENV and ZIKV in naive and DENV-preimmune subjects, with important implications for natural infections and vaccine development.
Authentication of Coffea arabica Varieties through DNA Fingerprinting and its Significance for the Coffee Sector
Authors: Pruvot-Woehl Solène , Krishnan Sarada , Solano William , Schilling Tim , Toniutti Lucile , Bertrand Benoit , Montagnon Christophe 
Published: March 2020

Abstract

Background
Locating the optimal varieties for coffee cultivation is increasingly considered a key condition for sustainable production and marketing. Variety performance varies when it comes to susceptibility to coffee leaf rust and other diseases, adaptation to climate change and high cup quality for specialty markets. But because of poor organization and the lack of a professional coffee seed sector, most existing coffee farms (and even seed lots and nurseries) do not know which varieties they are using. DNA fingerprinting of coffee planting material will contribute to professionalize the coffee seed sector.

Objective
The objective of this paper is i) to check in a large scale the robustness of the existing coffee DNA fingerprinting method based on eight Single Sequence Repeats markers (SRR) and ii) to describe how it can help in moving the needle towards a more professional seed sector.

Method
2533 samples representing all possible genetic background of Arabica varieties were DNA fingerprinted with 8 SRR markers. The genetic diversity was analyzed and the genetic conformity to varietal references was assessed.

Results
The DNA fingerprinting method proved to be robust in authenticating varieties and trace back the history of C. arabica breeding and of the movement of C. arabica varieties. The genetic conformity of two important coffee varieties, Marseillesa and Gesha, proved to be 91% and 39% respectively.

Conclusions
DNA fingerprinting provides different actors in the coffee sector with a powerful new tool—farmers can verify the identity of their cultivated varieties, coffee roasters can be assured that marketing claims related to varieties are correct, and most of all, those looking to establish the a more professional and reliable coffee seed sector have a reliable new monitoring tool to establish and check genetic purity of seed stock and nursery plants.

Highlights
While C. arabica is primarily self-pollinating, even fixed line varieties appear to be drifting away from their original genetic reference due to uncontrolled cross pollination. A set of 8 SSR markers applied to the largest possible genetically diverse set of samples prove to discriminate between a wide range of varieties Figures confirm that genetic non conformity of coffee varieties can represent up to 61% of checked samples.