bluuebunny/crossref_metadata_2025_split huggingface dataset:
The SF3b complex: splicing and beyond
Authors: Sun Chengfu 
Published: March 2020

AbstractThe SF3b complex is an intrinsic component of the functional U2 small nuclear ribonucleoprotein (snRNP). As U2 snRNP enters nuclear pre-mRNA splicing, SF3b plays key roles in recognizing the branch point sequence (BPS) and facilitating spliceosome assembly and activation. Since the discovery of SF3b, substantial progress has been made in elucidating its molecular mechanism during splicing. In addition, numerous recent studies indicate that SF3b and its components are engaged in various molecular and cellular events that are beyond the canonical role in splicing. This review summarizes the current knowledge on the SF3b complex and highlights its multiple roles in splicing and beyond.
A think-aloud study to inform the design of radiograph interpretation practice
Authors: Yoon Jong-Sung , Boutis Kathy , Pecaric Martin R. , Fefferman Nancy R. , Ericsson K. Anders , Pusic Martin V. 
Published: March 2020

AbstractModels for diagnostic reasoning in radiology have been based on the observed behaviors of experienced radiologists but have not directly focused on the thought processes of novices as they improve their accuracy of image interpretation. By collecting think-aloud verbal reports, the current study was designed to investigate differences in specific thought processes between medical students (novices) as they learn and radiologists (experts), so that we can better design future instructional environments. Seven medical students and four physicians with radiology training were asked to interpret and diagnose pediatric elbow radiographs where fracture is suspected. After reporting their diagnosis of a case, they were given immediate feedback. Participants were asked to verbalize their thoughts while completing the diagnosis and while they reflected on the provided feedback. The protocol analysis of their verbalizations showed that participants used some combination of four processes to interpret the case: gestalt interpretation, purposeful search, rule application, and reasoning from a prior case. All types of processes except reasoning from a prior case were applied significantly more frequently by experts. Further, gestalt interpretation was used with higher frequency in abnormal cases while purposeful search was used more often for normal cases. Our assessment of processes could help guide the design of instructional environments with well-curated image banks and analytics to facilitate the novice’s journey to expertise in image interpretation.
Development of a sensitive monoclonal antibody-based sandwich ELISA to detect Vip3Aa in genetically modified crops
Authors: Liu Weixiao , Liu Xuri , Liu Chao , Zhang Zhe , Jin Wujun 
Published: March 2020

Abstract
Objectives
To develop a sensitive monoclonal antibody-based sandwich enzyme-linked immunosorbent assay (ELISA) to detect Vip3Aa in genetically modified (GM) crops and their products.

Results
Vegetative insecticidal proteins (Vips) are secreted by Bacillus thuringiensis (Bt) and are known to be toxic to Lepidoptera species. Vip3Aa family proteins, Vip3Aa19 and Vip3Aa20, were successfully applied in GM crops to confer an effective and persistent insecticidal resistance. A sensitive monoclonal antibody-based sandwich ELISA was developed to detect Vip3Aa in GM crops and their products. Two monoclonal antibodies were raised against the overexpressed and purified His-Vip3Aa20, were purified from mouse ascites and characterized. A sandwich ELISA method was developed using the 2G3-1D7 monoclonal antibody for capture and the biotin-labeled 1F9-1F5 monoclonal antibody for detection of Vip3Aa20. The linear detection range of the method was found to be approximately 31.25–500 pg/ml, with a sensitivity of 10.24 pg/ml.

Conclusions
The established ELISA was effective for detecting Vip3Aa family proteins other than Vip3Aa8, and was successfully applied in the detection of Vip3Aa20 and Vip3Aa19 expressed in transgenic maize and cotton.
Practical relevance of software engineering research: synthesizing the community’s voice
Authors: Garousi Vahid , Borg Markus , Oivo Markku 
Published: March 2020

AbstractSoftware engineering (SE) research should be relevant to industrial practice. There have been regular discussions in the SE community on this issue since the 1980’s, led by pioneers such as Robert Glass. As we recently passed the milestone of “50 years of software engineering”, some recent positive efforts have been made in this direction, e.g., establishing “industrial” tracks in several SE conferences. However, many researchers and practitioners believe that we, as a community, are still struggling with research relevance and utility. The goal of this paper is to synthesize the evidence and experience-based opinions shared on this topic so far in the SE community, and to encourage the community to further reflect and act on the research relevance. For this purpose, we have conducted a Multi-vocal Literature Review (MLR) of 54 systematically-selected sources (papers and non peer-reviewed articles). Instead of relying on and considering the individual opinions on research relevance, mentioned in each of the sources, the MLR aims to synthesize and provide the “holistic” view on the topic. The highlights of our MLR findings are as follows. The top three root causes of low relevance, discussed in the community, are: (1) Researchers having simplistic views (or wrong assumptions) about SE in practice; (2) Lack of connection with industry; and (3) Wrong identification of research problems. The top three suggestions for improving research relevance are: (1) Using appropriate research approaches such as action-research; (2) Choosing relevant (practical) research problems; and (3) Collaborating with industry. By synthesizing all the discussions on this important topic so far, this paper aims to encourage further discussions and actions in the community to increase our collective efforts to improve the research relevance. Furthermore, we raise the need for empirically-grounded and rigorous studies on the relevance problem in SE research, as carried out in other fields such as management science.
Who Am I?: Using Reflective Practice and Self-determination to Redefine ‘Employability’ in Legal Education
Authors: Brooman Simon , Stirk Sarah 
Published: March 2020

AbstractThis study examines the value of undergraduate law students undertaking structured and assessed reflective practice as part of their studies, in the context of competing notions of employability. We discuss existing use of reflective practice in law and other disciplines, highlighting the different approaches and perspectives on its value. Students in this study engaged in reflection as part of a first year core module aimed at improving retention and performance, followed by a second year option module aimed at personal development in relation to employability and business skills following a work placement. We examine students’ perceptions of reflective practice and discuss how these relate to extant literature. We suggest that reflective practice is a very personal experience that can greatly benefit law students beyond their capacity to be employable. We suggest that reflective practice for law needs to be re-thought in relation to its intended purpose, taking cognisance of experience outside law to inform best practice. We propose that reflective practice should focus on developing the whole person, and discuss this in light of debate over the function of higher education. We suggest that the reinforcement cycle of personal and professional reflective practice in this study has the potential to be high impact practice in legal education as defined by Professor George Kuh.
Cross-Sectoral Mobility Funding and the Challenge of Immersion: The Case of SSH
Authors: Hellström Tomas , Hellström Christina 
Published: March 2020

AbstractProject funding rarely demands much change on behalf of the recipient. In contrast, cross-sectoral mobility funding requires recipients to change their environment and often some aspects of their research. There is a need to understand the impact on the researchers’ experiences as knowledge producers within such programs, as part of the broader potential and significance of cross-sectoral mobility funding. This study draws on interviews with participants of the Swedish ‘Flexit’ program in order to develop a framework for assessing the dynamics and efficacy of such funding instruments. To do this, we develop a framework for understanding their cognitive effects; especially their ‘immersive’ potential, i.e., their ability to naturally involve the participant in their new setting. It proposes two dimensions along which such an assessment can take place: how the instrument challenges participants’ knowledge production practices, and the level of immersion that participants are subject to as part of the program.
Biological barriers, and the influence of protein binding on the passage of drugs across them
Authors: Wanat Karolina 
Published: March 2020

AbstractDrug-protein binding plays a key role in determining the pharmacokinetics of a drug. The distribution and protein binding ability of a drug changes over a lifetime, and are important considerations during pregnancy and lactation. Although proteins are a significant fraction in plasma composition, they also exist beyond the bloodstream and bind with drugs in the skin, tissues or organs. Protein binding influences the bioavailability and distribution of active compounds, and is a limiting factor in the passage of drugs across biological membranes and barriers: drugs are often unable to cross membranes mainly due to the high molecular mass of the drug-protein complex, thus resulting in the accumulation of the active compounds and a significant reduction of their pharmacological activity. This review describes the consequences of drug-protein binding on drug transport across physiological barriers, whose role is to allow the passage of essential substances—such as nutrients or oxygen, but not of xenobiotics. The placental barrier regulates passage of xenobiotics into a fetus and protects the unborn organism. The blood–brain barrier is the most important barrier in the entire organism and the skin separates the human body from the environment.
Turbulent travel speeds in nonlinear vehicle dynamics
Authors: Wedig Walter V. 
Published: March 2020

AbstractQuarter car models of vehicles rolling on wavy roads lead to limit cycles of travel speed and acceleration with period doublings and bifurcation effects for appropriate driving force parameters. In case of narrow-banded road excitations, speed jumps occur, additionally. This has the consequence that the driving speed becomes turbulent. Bifurcation and jump effects vanish with growing vehicle damping. The same happens for increasing bandwidth of road excitations when, e.g., on flat highways there are no big road waves but only small noisy slope processes generated by rough road surfaces. The paper derives a new stability condition in mean. Numerical time integrations are stabilized by means of polar coordinates. Equivalently, Fourier series expansions are introduced in the angle domain. Phase portraits of travel speed and acceleration show new period-doublings of limit cycles when speed gets stuck before resonance. The paper extends these investigations to the stochastic case that road surfaces are random generated by filtered white noise. By means of Gaussian closure, a nonlinear mean speed equation is derived which includes the extreme cases of wavy roads and road noise.
Predicting the effects of anthropogenic noise on fish reproduction
Authors: de Jong Karen , Forland Tonje Nesse , Amorim Maria Clara P. , Rieucau Guillaume , Slabbekoorn Hans , Sivle Lise Doksæter 
Published: March 2020

AbstractAquatic animals use and produce sound for critical life functions, including reproduction. Anthropogenic noise is recognized as a global source of environmental pollution and adequate conservation and management strategies are urgently needed. It becomes therefore critical to identify the reproductive traits that render a species vulnerable to acoustic disturbances, and the types of anthropogenic noise that are most likely to impact reproduction. Here, we provide predictions about noise impact on fish reproduction following a two-step approach: first, we grouped documented effects of noise into three mechanistic categories: stress, masking and hearing-loss, and test which type of noise (continuous vs intermittent and regular vs irregular) was most likely to produce a significant response in each category with either a meta-analysis or a quantitative review, depending on data availability. Second, we reviewed existing literature to predict which reproductive traits would render fish most sensitive to stress, masking and hearing-loss. In step one, we concluded that continuous sounds with irregular amplitude and/or frequency-content (e.g. heavy ship traffic) were most likely to cause stress, and continuous sounds were also most likely to induce masking and hearing-loss. From step two we concluded that the vulnerability of a species to noise-induced stress will mainly depend on: (1) its potential to reallocate reproduction to more quiet times or locations, and (2) its vulnerability to masking and hearing-loss mainly on the function of sound communication in its reproductive behaviour. We discuss in which stages of reproduction fish are most likely to be vulnerable to anthropogenic noise based on these findings.Graphic abstract
Joint attention and perceptual experience
Authors: Battich Lucas , Geurts Bart 
Published: March 2020

AbstractJoint attention customarily refers to the coordinated focus of attention between two or more individuals on a common object or event, where it is mutually “open” to all attenders that they are so engaged. We identify two broad approaches to analyse joint attention, one in terms of cognitive notions like common knowledge and common awareness, and one according to which joint attention is fundamentally a primitive phenomenon of sensory experience. John Campbell’s relational theory is a prominent representative of the latter approach, and the main focus of this paper. We argue that Campbell’s theory is problematic for a variety of reasons, through which runs a common thread: most of the problems that the theory is faced with arise from the relational view of perception that he endorses, and, more generally, they suggest that perceptual experience is not sufficient for an analysis of joint attention.
TeKET: a Tree-Based Unsupervised Keyphrase Extraction Technique
Authors: Rabby Gollam , Azad Saiful , Mahmud Mufti , Zamli Kamal Z. , Rahman Mohammed Mostafizur 
Published: March 2020

AbstractAutomatic keyphrase extraction techniques aim to extract quality keyphrases for higher level summarization of a document. Majority of the existing techniques are mainly domain-specific, which require application domain knowledge and employ higher order statistical methods, and computationally expensive and require large train data, which is rare for many applications. Overcoming these issues, this paper proposes a new unsupervised keyphrase extraction technique. The proposed unsupervised keyphrase extraction technique, named TeKET or Tree-based Keyphrase Extraction Technique, is a domain-independent technique that employs limited statistical knowledge and requires no train data. This technique also introduces a new variant of a binary tree, called KeyPhrase Extraction (KePhEx) tree, to extract final keyphrases from candidate keyphrases. In addition, a measure, called Cohesiveness Index or CI, is derived which denotes a given node’s degree of cohesiveness with respect to the root. The CI is used in flexibly extracting final keyphrases from the KePhEx tree and is co-utilized in the ranking process. The effectiveness of the proposed technique and its domain and language independence are experimentally evaluated using available benchmark corpora, namely SemEval-2010 (a scientific articles dataset), Theses100 (a thesis dataset), and a German Research Article dataset, respectively. The acquired results are compared with other relevant unsupervised techniques belonging to both statistical and graph-based techniques. The obtained results demonstrate the improved performance of the proposed technique over other compared techniques in terms of precision, recall, and F1 scores.
Linking field survey with crop modeling to forecast maize yield in smallholder farmers’ fields in Tanzania
Authors: Liu Lin , Basso Bruno 
Published: March 2020

AbstractShort term food security issues require reliable crop forecasting data to identify the population at risk of food insecurity and quantify the anticipated food deficit. The assessment of the current early warning and crop forecasting system which was designed in mid 80’s identified a number of deficiencies that have serious impact on the timeliness and reliability of the data. We developed a new method to forecast maize yield across smallholder farmers’ fields in Tanzania (Morogoro, Kagera and Tanga districts) by integrating field-based survey with a process-based mechanistic crop simulation model. The method has shown to provide acceptable forecasts (r2 values of 0.94, 0.88 and 0.5 in Tanga, Morogoro and Kagera districts, respectively) 14–77 days prior to crop harvest across the three districts, in spite of wide range of maize growing conditions (final yields ranged from 0.2–5.9 t/ha). This study highlights the possibility of achieving accurate yield forecasts, and scaling up to regional levels for smallholder farming systems, where uncertainties in management conditions and field size are large.
Groundwater flow system understanding of the lukewarm springs in Kistapolca (South Hungary) and its relevance to hypogene cave formation
Authors: Erőss Anita , Csondor Katalin , Czuppon György , Dezső József , Müller Imre 
Published: March 2020

AbstractSprings have an important role both in groundwater flow system understanding and in maintaining groundwater-related ecosystems. The aim of the research of the lukewarm karst springs in Kistapolca is to understand the origin of the elevated temperature (22–24 °C), i.e., whether it is the result of mixing of cold and thermal waters or it represents the terminal area of intermediate flow systems. This question has also an important relevance to the evaluation of recent cave formation in the area. In case of mixing of different waters dissolution by mixing corrosion could take place. This scenario was proposed for the formation of the Beremend Cave, which is situated in the close vicinity of the springs, and which is the only known cave of the area, where the passages are partly filled by water. Therefore, several methods, including geophysical, geochemical and stable isotope measurements, evaluation of continuous-time series of water level change, temperature, and electrical conductivity data and hydraulic evaluation of vertical flow conditions by pressure–elevation profiles were combined in order to acquire information about the hydrogeological environment and flow conditions in the area. The results show that a higher order flow system derived from the Villány Hills feeds the springs in Kistapolca without significant local influence. In addition, the results related to the Beremend Cave indicate that cave formation by mixing corrosion is not an active process today in the area, the cave is rather influenced by local processes.
Conceptualizing Individual and Household Disaster Preparedness: The Perspective from Cameroon
Authors: Nojang Emmanuel Nzengung , Jensen Jessica 
Published: March 2020

AbstractThis research explored the basic issue of what preparedness means and entails to people in Fako Division, Cameroon—a place threated by many hazards and that has experienced many disasters. Findings from the analysis of the 33 interviews conducted in this study indicate that preparedness is a dynamic state of readiness that is dependent on context, a social process, and a process of completing activities to save lives and minimize the effects of disasters. In addition, the research determined that Cameroonians view a wholly prepared person as someone who would: (1) have knowledge about hazards and what to do about them; (2) engage others, including their families and neighbors, in discussions about activities related to hazards; and (3) participate in activities to minimize loss from hazards, sustain themselves in the face of hazards, and flee from hazards. The findings from the interview data synchronize to a large extent with what is implied, but not clearly stated, in the existing research literature. The article addresses this synchrony, posits a definition of preparedness, and identifies the theoretical components of preparedness.
Psychosocial resources related to survival among non-robust community-dwelling older people: an 18-year follow-up study
Authors: Lavonius Sirkku , Salminen Marika , Vahlberg Tero , Isoaho Raimo , Kivelä Sirkka-Liisa , Wuorela Maarit , Löppönen Minna , Viitanen Matti , Viikari Laura 
Published: March 2020

Abstract
Purpose
Psychosocial resources have been considered to be associated with survival among frail older adults but the evidence is scarce. The aim was to investigate whether psychosocial resources are related to survival among non-robust community-dwelling older people.

Methods
This is a prospective study with 10- and 18-year follow-ups. Participants were 909 non-robust (according to Rockwood’s Frailty Index) older community-dwellers in Finland. Psychosocial resources were measured with living circumstances, education, satisfaction with friendship and life, visiting other people, being visited by other people, having someone to talk to, having someone who helps, self-rated health (SRH) and hopefulness about the future. To assess the association of psychosocial resources for survival, Cox regression analyses was used.

Results
Visiting other people more often than once a week compared to that of less than once a week (hazard ratio 0.61 [95% confidence interval 0.44–0.85], p = 0.003 in 10-year follow-up; 0.77 [0.62–0.95], p = 0.014 in 18-year follow-up) and good SRH compared to poor SRH (0.65 [0.44–0.97], p = 0.032; 0.68 [0.52–0.90], p = 0.007, respectively) were associated with better survival in both follow-ups. Visiting other people once a week (compared to that of less than once a week) (0.77 [0.62–0.95], p = 0.014) was only associated with better 18-year survival.

Conclusions
Psychosocial resources, such as regularly visiting other people and good self-rated health, seem to be associated with better survival among non-robust community-dwelling Finnish older people. This underlines the importance of focusing also on psychosocial well-being of frail older subjects to remain or promote their resilience.
Unfolding the Black Box of Questionable Research Practices: Where Is the Line Between Acceptable and Unacceptable Practices?
Authors: Linder Christian , Farahbakhsh Siavash 
Published: March 2020

ABSTRACTDespite the extensive literature on what questionable research practices (QRPs) are and how to measure them, the normative underpinnings of such practices have remained less explored. QRPs often fall into a grey area of justifiable and unjustifiable practices. Where to precisely draw the line between such practices challenges individual scholars and this harms science. We investigate QRPs from a normative perspective using the theory of communicative action. We highlight the role of the collective in assessing individual behaviours. Our contribution is a framework that allows identification of when particular actions cross over from acceptable to unacceptable practice. Thus, this article provides grounds for developing scientific standards to raise the quality of scientific research.