bluuebunny/crossref_metadata_2025_split huggingface dataset:
Antibiotic resistance genes from livestock waste: occurrence, dissemination, and treatment
Authors: He Ya , Yuan Qingbin , Mathieu Jacques , Stadler Lauren , Senehi Naomi , Sun Ruonan , Alvarez Pedro J. J. 
Published: February 2020

AbstractAntibiotics are widely used in animal husbandry, and various types of antibiotic resistance genes (ARGs) are frequently detected in livestock waste around the world. Conventional livestock waste treatment processes do not completely remove ARGs, resulting in their release to soil and water environments. Various exposure routes of these ARGs to humans, including inhalation and ingestion of antibiotic-resistant bacteria (ARB) that harbor them, may be contributing to the rise in resistant clinical infections that are increasingly difficult to treat with antibiotics. In this review, we assess the occurrence and variability of ARGs in livestock wastes and their potential propagation pathways to human pathogens. We also review the mechanisms and environmental factors that influence the dissemination of ARGs through these pathways, and evaluate the ARG removal efficiency of common livestock waste management approaches. Challenges and research needs for assessing and mitigating the risk of antibiotic resistance dissemination from livestock waste are also presented.
Design and synthesis of new energy restriction mimetic agents: Potent anti-tumor activities of hybrid motifs of aminothiazoles and coumarins
Authors: Hersi Fatema , Omar Hany A. , Al-Qawasmeh Raed A. , Ahmad Zainab , Jaber Areej M. , Zaher Dana M. , Al-Tel Taleb H. 
Published: February 2020

AbstractThe incidence of obesity-related diseases like diabetes, cardiovascular diseases, and different types of cancers shed light on the importance of dietary control as preventive and treatment measures. However, long-term dietary control is challenging to achieve in most individuals. The use of energy restriction mimetic agents (ERMAs) as an alternative approach to affect the energy machinery of cancer cells has emerged as a promising approach for cancer therapy. ERMAs limit the high need for energy in rapidly growing tumor cells, with their survival rate strongly dependent on the robust availability of energy. In this context, initial phenotypic screening of an in-house pilot compound library identified a new class of aminothiazole anchored on coumarin scaffold as potent anticancer lead drug candidates with potential activity as ERMA. The identified chemotypes were able to inhibit glucose uptake and increase ROS content in cancer cells. Compounds 9b, 9c, 9i, 11b, and 11c were highly active against colorectal cancer cell lines, HCT116 and HT-29, with half-maximal inhibitory concertation (IC50) range from 0.25 to 0.38 µM. Further biological evaluations of 9b and 9f using Western blotting, caspase activity, glucose uptake, ROS production, and NADPH/NADP levels revealed the ability of these lead drug candidates to induce cancer cell death via, at least in part, energy restriction. Moreover, the assessment of 9b and 9f synergistic activity with cisplatin showed promising outcomes. The current work highlights the significant potential of the lead compounds, 9b, and 9f as potential anticancer agents via targeting the cellular energy machinery in cancer cells.
Sequential delayed [18 F]FDG PET/CT examinations in the pharynx
Authors: Pietrzak Agata Karolina , Marszalek Andrzej , Kazmierska Joanna , Kunikowska Jolanta , Golusinski Pawel , Suchorska Wiktoria Maria , Michalak Marcin , Cholewinski Witold 
Published: February 2020

AbstractThis study aimed to evaluate the usefulness of the biphasic 2-deoxy-2-[18 F]fluoro-D-glucose positron emission tomography/computed tomography ([18 F]FDG PET/CT) examinations in terms of distinguishing benign and malignant lesions within the pharynx. 139 patients underwent sequential biphasic [18 F]FDG PET/CT examinations at 60 and 90 minutes (min) post intravenous injection (p.i.) of the [18 F]FDG. We evaluated the metabolic activity of 93 malignant lesions and 59 benign findings within pharynx as well as 70 normal blood vessels. We evaluated the maximal and mean standardized uptake value (SUVmax, SUVmean) and the retention index (RI-SUVmax). We used the receiver operating characteristics (ROC) analysis to obtain the prognostic metabolic indices cut-off which may differentiate between benign and malignant lesions. The SUVmax value cut-off at 60 and 90 min p.i. differentiating between normal and abnormal metabolic activity in the pharynx was 1.9 and 2.0, respectively. When compared benign and malignant lesions, the SUVmax on initial and delayed scans were 3.1 and 3.6, respectively. In this material, the increase of the SUVmax value over time of 1.7% suggested abnormality, while RI-SUVmax of 5.7% indicated malignant etiology. The biphasic [18 F]FDG PET/CT study protocol is useful in better stratification of normal and abnormal glucose metabolism activity in the pharynx.
Linking midlatitudes eddy heat flux trends and polar amplification
Authors: Chemke Rei , Polvani Lorenzo M. 
Published: February 2020

AbstractEddy heat fluxes play the important role of transferring heat from low to high latitudes, thus affecting midlatitude climate. The recent and projected polar warming, and its effects on the meridional temperature gradients, suggests a possible weakening of eddy heat fluxes. We here examine this question in reanalyses and state-of-the-art global climate models. In the Northern Hemisphere we find that the eddy heat flux has robustly weakened over the last four decades. We further show that this weakening emerged from the internal variability around the year 2000, and we attribute it to increasing greenhouse gases. In contrast, in the Southern Hemisphere we find that the eddy heat flux has robustly strengthened, and we link this strengthening to the recent multi-decadal cooling of Southern-Ocean surface temperatures. The inability of state-of-the-art climate models to simulate such cooling prevents them from capturing the observed Southern Hemisphere strengthening of the eddy heat flux. This discrepancy between models and reanalyses provides a clear example of how model biases in polar regions can affect the midlatitude climate.
Tools and analytic techniques to synthesise community knowledge in CBPR using computer-mediated participatory system modelling
Authors: Hayward Joshua , Morton Saraya , Johnstone Michael , Creighton Doug , Allender Steven 
Published: February 2020

AbstractParticipatory systems thinking methods are often used in community-based participatory research to engage and respond to complexity. Participation in systems thinking activities creates opportunities for participants to gain useful insights about complexity. It is desirable to design activities that extend the benefits of this participation into communities, as these insights are predictive of success in community-based prevention. This study tests an online, computer-mediated participatory system modelling platform (STICKE) and associated methods for collating and analysing its outputs. STICKE was trialled among a group of community members to test a computer-mediated system modelling exercise. The causal diagrams resulting from the exercise were then merged, and network analysis and DEMATEL methods applied to inform the generation of a smaller summary model to communicate insights from the participant group as a whole. Participants successfully completed the online modelling activity, and created causal diagrams consistent with expectations. The DEMATEL analysis was identified as the participant-preferred method for converging individuals causal diagrams into a coherent and useful summary. STICKE is an accessible tool that enabled participants to create causal diagrams online. Methods trialled in this study provide a protocol for combining and summarising individual causal diagrams that was perceived to be useful by the participant group. STICKE supports communities to consider and respond to complex problems at a local level, which is cornerstone of sustainable effective prevention. Understanding how communities perceive their own health challenges will be important to better support and inform locally owned prevention efforts.
Methodological nationalism in translation studies
Authors: Cussel Mattea 
Published: February 2020

AbstractThis article critiques methodological nationalism and binaries in theoretical discussions of literary translation. The naturalization of the national story of translation is traced from the Renaissance up to its uncritical adoption when the discipline of translation studies was established. Borrowing from critiques of methodological nationalism in other disciplines, it is argued that a thorough revision of certain vocabularies is still needed to definitively break with lingering national and binary tropes. Venuti’s foreignization is challenged due to its most problematic but previously overlooked aspect: its reliance on national paradigms and circumscribed domestic and foreign groups. To eschew the image of literary translation as transfer from culture A to culture B, an alternative empirical approach to networks of intersectionally-positioned readers in transnational localities is proposed. This critique is necessary given the messiness of subjectivity and the need for new solidarities in our transforming transnational world.
Butterfly-wing pollination inScadoxusand other South African Amaryllidaceae
Authors: Butler Hannah C , Johnson Steven D 
Published: February 2020

AbstractUnderstanding the evolution of floral morphology requires information about the identity of pollinators as well as the specific mechanisms of pollen transfer. Based on preliminary field observations and floral structure, we hypothesized that pollination mechanisms involving the transfer of pollen on butterfly wings occur in several lineages of South African Amaryllidaceae. Here we report findings from a detailed study of butterfly-wing pollination in two subspecies of Scadoxus multiflorus and review the prevalence of this pollination mechanism among other Amaryllidaceae in southern Africa. We established that S. multiflorus subsp. katherinae is genetically self-incompatible and thus entirely reliant on pollinators for seed production. We determined that this subspecies is pollinated almost exclusively by large swallowtail butterflies, principally males of the mocker swallowtail Papilio dardanus cenea. Flowers of S. multiflorus subsp. multiflorus are pollinated by pierid and swallowtail butterflies. Pollen is deposited on the ventral surface of the wings of butterflies as they flutter over the strongly exserted stamens. We predict that butterfly-wing pollination occurs in at least nine species of South African Amaryllidaceae, which may reflect several independent origins of this mechanism. The flowers of these species are red or orange with strong herkogamy and are either bowl-brush or open-brush in shape. We provide maps of the distribution of pollen on the ventral surface of the wings of pollinators for four of these species. All four appear to be pollinated via the ventral surface of large butterfly wings, with the floral structure facilitating the process. These findings illustrate the importance of investigating pollen transfer mechanisms in order to understand patterns of floral diversification and floral convergence.
Leveraging Integrated Continuous Manufacturing to Address Critical Issues in the U.S. Military
Authors: Takizawa Bayan Teisho , Born Stephen Christopher , Mascia Salvatore 
Published: February 2020

ABSTRACT
There is a tremendous opportunity to modernize the pharmaceutical manufacturing industry—relinquishing outdated machines that have been used for decades, and replacing them with state-of-the-art equipment that reflect more contemporary advanced technologies. This article describes how the implementation of continuous manufacturing, replacing outdated batch systems, can positively impact our health care sector. Important benefits will include the creation of advanced pharmaceutical manufacturing jobs in the United States, the establishment of capabilities and capacity to quickly produce drugs critical to U.S. citizens, the reduction of health care costs through more efficient manufacturing, and access to better quality drugs through more sophisticated and reliable production processes. Furthermore, the application of continuous manufacturing will enable the U.S. Government, in partnership with pharmaceutical companies, to address current issues such as drug shortages, national emergencies (eg, natural disasters or chemical, biological, radiological, or nuclear threats), the Strategic National Stockpile (ie, improving response time and reducing maintenance costs), and the delivery of critical drugs to distant geographies (eg, forward military bases). The article also provides a detailed example of a critical aspect of continuous manufacturing: the ability to overcome technical challenges encountered by batch technologies.
Scrambler Therapy Treatment: The Importance of Examining Clinically Meaningful Improvements in Chronic Pain and Quality of Life
Authors: Nayback-Beebe Ann , Panula Tanya , Arzola Sonya , Goff Brandon 
Published: February 2020

Abstract

Introduction
Calmare Scrambler Therapy (ST) interferes with pain signal transmission by using nerve fibers to convey a message of normality to the central nervous system. This prospective, double-blinded, randomized trial had three aims. First, we tried to determine ST’s effectiveness in reducing chronic neuropathic pain symptoms and analgesic medication use in military service members, when compared to sham treatment. Next, we examined its effect on reported mental and physical health-related quality of life. Finally, we sought to describe participant perceptions of treatment effectiveness.

Materials and Methods
Forty-seven subjects were randomized to receive ten 30-minute active ST or sham treatments. Data were collected at baseline, posttreatment, and 1-month follow-up.

Results
The groups showed no statistically significant differences in pain scores, medication use, or mental or physical health-related quality of life with active versus sham treatment. However, both produced clinically meaningful reductions in pain and improvements in physical health-related quality posttreatment that was sustained at 1-month follow-up. Ninety percent of the blinded sample described the treatment intervention as a partial or complete success.

Conclusion
ST is no better than sham treatment in decreasing pain. Yet, patient perceptions of treatment effectiveness are equally important in chronic pain treatment.
Pediatric Prehospital Wound Prophylaxis in Iraq and Afghanistan
Authors: Naylor Jason F , April Michael D , Hill Guyon J , Schauer Steven G 
Published: February 2020

Abstract

Background
Infectious complications of war wounds are a significant source of mortality and morbidity. Tactical Combat Casualty Care (TCCC) guidelines recommend prehospital moxifloxacin, ertapenem, or cefotetan for “all open combat wounds.” We describe the prehospital administration of antibiotics to pediatric trauma patients.

Methods
We queried the Department of Defense Trauma Registry for all pediatric subjects admitted to United States and Coalition fixed-facility hospitals in Iraq and Afghanistan from January 2007 to January 2016.

Results
During this time, there were 3,439 pediatric encounters which represented 8.0% of all admissions. Prehospital providers administered a total of 216 antibiotic doses to 210 subjects. Older children received antibiotics more frequently than younger children, were more likely to be male, located in Afghanistan, and injured by explosive with the majority surviving to hospital discharge. Cefazolin and ceftriaxone were the most frequently utilized antibiotics.

Conclusions
The most frequently administered antibiotics were cephalosporins. TCCC recommended agents for adult prehospital wound prophylaxis were infrequently administered to pediatric casualties. Administration rates of pediatric prehospital wound prophylaxis may be improved with pediatric-specific TCCC guidelines recommending cephalosporins as first-line agents, fielding of a TCCC-oriented Broselow tape, and training prehospital providers on administration of antimicrobials.
Relationship of Anthropometric Measures on Female Trainees’ and Active Duty Soldiers’ Performance of Common Soldiering Tasks
Authors: Redmond Jan E , Cohen Bruce S , Haven Caitlin C , Pierce Joseph R , Foulis Stephen A , Frykman Peter N , Canino Maria C , Sharp Marilyn A 
Published: February 2020

Abstract

Introduction
This study compared the relationship between height (HT), body mass (BM), and body mass index (BMI) of female trainees and active duty female soldiers and their performance on simulated common soldiering tasks (CSTs) with high physical demands.

Methods
Female trainees (n = 133) and soldiers (n = 229) completed the following CSTs: sandbag carry, move under fire, casualty drag, casualty evacuation, and road march. Quartiles were created among HT, BM, and BMI by which task performance was compared using ANOVAs with Tukey post hoc comparisons.

Results
For both trainees and soldiers, HT, BM, and BMI were positively associated with improved road march, casualty drag, casualty evacuation, and sandbag carry performance. On the move under fire task, only soldier HT was positively associated with improved performance.

Conclusion
Female trainees and soldiers who are taller and heavier with a higher BMI may demonstrate better performance on CSTs required of all soldiers. In addition to task-specific training, performance of CSTs may be enhanced in tasks requiring strength and power by recruiting and retaining taller and heavier females with a higher BMIs. Allowances should be considered for soldiers and trainees who can successfully perform soldiering tasks with high physical demands despite less desirable anthropometric measurements.
How comets work: nucleus erosion versus dehydration
Authors: Fulle Marco , Blum J , Rotundi A , Gundlach B , Güttler C , Zakharov V 
Published: February 2020

ABSTRACT
We develop an activity model based on ice sublimation and gas diffusion inside cm-sized pebbles making-up a cometary nucleus. Our model explains cometary activity assuming no free parameters and fixing the nucleus surface temperature Ts, its gradient below the nucleus surface at thermal equilibrium, the pressure inside the porous pebbles, and the gas flux from them. We find that (i) the nucleus erosion rate and water vapour flux are independent of the nucleus refractory-to-ice ratio, which affects the dehydration rate only; (ii) water-driven dust ejection occurs in thermal quasi-equilibrium at Ts &gt; 205 K; (iii) the smallest and largest ejected dust sizes depend on the nucleus surface temperature and its gradient at depths of few cm; and (iv) the water-driven nucleus erosion rate is independent of the water vapour flux. Regarding comet 67P/Churyumov–Gerasimenko, we find that (i) during the northern and southern polar summers, the nucleus active areas are ≈5 km2; (ii) &gt;95 per cent of the southern pristine nucleus has a refractory-to-water-ice mass ratio &gt;5; and (iii) the different temperature dependences of the dehydration and erosion rates explain the seasonal cycle: at perihelion, dm-sized chunks ejected by the sublimation of CO2 ices are rapidly enveloped by an insulating crust, preserving most water ice up to their fallout on the northern dust deposits; the inbound water-driven activity at low temperatures triggers a complete erosion of the fallout if its water-ice mass fraction is &gt;0.1 per cent.
Exonuclease combinations reduce noises in 3D genomics technologies
Authors: Kong Siyuan , Li Qing , Zhang Gaolin , Li Qiujia , Huang Qitong , Huang Lei , Zhang Hui , Huang Yinghua , Peng Yanling , Qin Baoming , Zhang Yubo 
Published: February 2020

Abstract
Chromosome conformation-capture technologies are widely used in 3D genomics; however, experimentally, such methods have high-noise limitations and, therefore, require significant bioinformatics efforts to extract reliable distal interactions. Miscellaneous undesired linear DNAs, present during proximity-ligation, represent a main noise source, which needs to be minimized or eliminated. In this study, different exonuclease combinations were tested to remove linear DNA fragments from a circularized DNA preparation. This method efficiently removed linear DNAs, raised the proportion of annulation and increased the valid-pairs ratio from ∼40% to ∼80% for enhanced interaction detection in standard Hi-C. This strategy is applicable for development of various 3D genomics technologies, or optimization of Hi-C sequencing efficiency.
Recombination may occur in the absence of transcription in the immunoglobulin heavy chain recombination centre
Authors: Oudinet Chloé , Braikia Fatima-Zohra , Dauba Audrey , Khamlichi Ahmed Amine 
Published: February 2020

AbstractDeveloping B cells undergo V(D)J recombination to generate a vast repertoire of Ig molecules. V(D)J recombination is initiated by the RAG1/RAG2 complex in recombination centres (RCs), where gene segments become accessible to the complex. Whether transcription is the causal factor of accessibility or whether it is a side product of other processes that generate accessibility remains a controversial issue. At the IgH locus, V(D)J recombination is controlled by Eμ enhancer, which directs the transcriptional, epigenetic and recombinational events in the IgH RC. Deletion of Eμ enhancer affects both transcription and recombination, making it difficult to conclude if Eμ controls the two processes through the same or different mechanisms. By using a mouse line carrying a CpG-rich sequence upstream of Eμ enhancer and analyzing transcription and recombination at the single-cell level, we found that recombination could occur in the RC in the absence of detectable transcription, suggesting that Eμ controls transcription and recombination through distinct mechanisms. Moreover, while the normally Eμ-dependent transcription and demethylating activities were impaired, recruitment of chromatin remodeling complexes was unaffected. RAG1 was efficiently recruited, thus compensating for the defective transcription-associated recruitment of RAG2, and providing a mechanistic basis for RAG1/RAG2 assembly to initiate V(D)J recombination.
DeepMicrobes: taxonomic classification for metagenomics with deep learning
Authors: Liang Qiaoxing , Bible Paul W , Liu Yu , Zou Bin , Wei Lai 
Published: February 2020

AbstractLarge-scale metagenomic assemblies have uncovered thousands of new species greatly expanding the known diversity of microbiomes in specific habitats. To investigate the roles of these uncultured species in human health or the environment, researchers need to incorporate their genome assemblies into a reference database for taxonomic classification. However, this procedure is hindered by the lack of a well-curated taxonomic tree for newly discovered species, which is required by current metagenomics tools. Here we report DeepMicrobes, a deep learning-based computational framework for taxonomic classification that allows researchers to bypass this limitation. We show the advantage of DeepMicrobes over state-of-the-art tools in species and genus identification and comparable accuracy in abundance estimation. We trained DeepMicrobes on genomes reconstructed from gut microbiomes and discovered potential novel signatures in inflammatory bowel diseases. DeepMicrobes facilitates effective investigations into the uncharacterized roles of metagenomic species.
The Tohono O’odham “Attack” on El Plomo: A Study in Sovereignty, Survivance, Security, and National Identity at the Dawn of the American Century
Authors: Bess Jennifer 
Published: February 2020

Abstract
Having inhabited the Sonoran Desert since time immemorial, the Tohono O’odham had been moving their herds of cattle across the U.S-Mexico border since they began ranching. But in 1898, within the context of the Spanish-American War, their migrations and subsequent local conflicts became national news, inciting the intervention of four government agencies.