bluuebunny/crossref_metadata_2025_split huggingface dataset:
Extracellular Microenvironmental Change by B16F10 Melanoma-derived Proteins Induces Cancer Stem-like Cell Properties from NIH3T3 Cells
Authors: Park Soon Yong , Lee Dong Gwang , Jo Ara , Choi Hyeongrok , Lee Joo Eon , Jeong Ae Jin , Leem Sun-Hee , Jun Woojin , Shim Sangin , Ye Sang-Kyu , Min Jeong-Ki , Chung Jin Woong 
Published: November 2019

AbstractCancer stem-like cells (CSCs) can generate solid tumors through the properties of stem cells such as self-renewal and differentiation and they cause drug resistance, metastasis and recurrence. Therefore, establishing CSC lines is necessary to conduct various studies such as on the identification of CSC origin and specific targeted therapies. In this study, we stimulated NIH3T3 fibroblasts to exhibit the characteristics of CSCs using the whole protein lysates of B16F10 melanoma cells. As a result, we induced colony formation that displayed self-renewal and differentiation capacities through anchorage-independent culture and re-attached culture. Moreover, colonies showed drug resistance by being maintained in the G0/G1 state. Colonies expressed various CSC markers and displayed high-level drug efflux capacity. Additionally, colonies clearly demonstrated tumorigenic ability by forming a solid tumor in vivo. These results show that proteins of cancer cells could transform normal cells into CSCs by increasing expression of CSC markers. This study argues the tremendous importance of the extracellular microenvironmental effect on the generation of CSCs. It also provides a simple experimental method for deriving CSCs that could be based on the development of targeted therapy techniques.
Effect of Substrate Surface Roughening on the Capacitance and Cycling Stability of Ni(OH)2 Nanoarray Films
Authors: Pimsawat Adulphan , Tangtrakarn Apishok , Pimsawat Nutsupa , Daengsakul Sujittra 
Published: November 2019

AbstractThe effect of substrate surface roughening on the capacitance of Ni(OH)2/NiOOH nanowall array samples produced via chemical bath deposition for 2, 4, 6, 24 and 48 h on an as-received stainless steel substrate and the same substrate after sandblasting has been investigated. Symmetric cells were subjected to 120,000 charge-discharge cycles to access changes in their capacitance. Specific capacitances were derived from cyclic voltammetry and charge-discharge cycling under a three electrode setup. Substrate roughening significantly increases the capacitance of symmetric cells and film stability since film exfoliation does not occur to the same degree as on the as-received substrate. Interestingly, films deposited on a roughened substrate for 6, 24 and 48 h also exhibit self-recovery of capacitance, which could be related to an electrodissolution-electrodeposition effect. With the use of a roughened substrate, the thinnest film gives the highest specific capacitance, 1456 F g−1, whilst the thickest one shows the highest areal capacitance, 235 mF cm−2, after 20,000 cycles. These results reveal the promise of surface roughening toward increasing the capacitance and stability of Ni(OH)2/NiOOH films.
Transcription factor Znf2 coordinates with the chromatin remodeling SWI/SNF complex to regulate cryptococcal cellular differentiation
Authors: Lin Jianfeng , Zhao Youbao , Ferraro Aileen R. , Yang Ence , Lewis Zachary A. , Lin Xiaorong 
Published: November 2019

AbstractCellular differentiation is instructed by developmental regulators in coordination with chromatin remodeling complexes. Much information about their coordination comes from studies in the model ascomycetous yeasts. It is not clear, however, what kind of information that can be extrapolated to species of other phyla in Kingdom Fungi. In the basidiomycete Cryptococcus neoformans, the transcription factor Znf2 controls yeast-to-hypha differentiation. Through a forward genetic screen, we identified the basidiomycete-specific factor Brf1. We discovered Brf1 works together with Snf5 in the SWI/SNF chromatin remodeling complex in concert with existent Znf2 to execute cellular differentiation. We demonstrated that SWI/SNF assists Znf2 in opening the promoter regions of hyphal specific genes, including the ZNF2 gene itself. This complex also supports Znf2 to fully associate with its target regions. Importantly, our findings revealed key differences in composition and biological function of the SWI/SNF complex in the two major phyla of Kingdom Fungi.
The association of AGO1 (rs595961G&gt;A, rs636832A&gt;G) and AGO2 (rs11996715C&gt;A, rs2292779C&gt;G, rs4961280C&gt;A) polymorphisms and risk of recurrent implantation failure
Authors: Ryu Chang Soo , Kim Young Ran , Kim Jung Oh , An Hui Jeong , Cho Sung Hwan , Ahn Eun Hee , Kim Ji Hyang , Lee Woo Sik , Kim Nam Keun 
Published: November 2019

Abstract
Recurrent implantation failure (RIF) is a common reproductive clinical condition treated by fertility specialists at in vitro fertilization (IVF) clinics. Several factors affect embryo implantation including the age of the female, the quality of embryos and the sperm, genetics, immunologic factors. Here, we investigated the association of Argonaute 1 (AGO1) and Argonaute 2 (AGO2) polymorphisms and RIF. We collected blood samples from 167 patients with RIF and 211 controls. Genetic polymorphisms were detected by polymerase chain reaction (PCR) – restriction fragment length polymorphism analysis and real-time PCR. We found that the AGO2 rs4961280C&gt;A polymorphism (adjusted odds ratio [AOR] = 1.984; P = 0.023) was significantly associated with RIF. Furthermore, in RIF patients with three or more consecutive implantation failure, the AGO2 rs4961280C&gt;A CA genotype (AOR = 2.133; P = 0.013) and dominant model (AOR = 2.272; P = 0.006) were both significantly associated with prevalence of RIF. An analysis of variance revealed that patients with the AGO2 rs2292779C&gt;G genotypes (CC: 6.52 ± 2.55; CG: 7.46 ± 3.02; GG: 8.42 ± 2.74; P = 0.044) and the dominant model (CC: 6.52 ± 2.55; CG+GG: 7.70 ± 2.97; P = 0.029) exhibited significantly increased white blood cell levels. Furthermore, patients with the AGO1 rs595961G&gt;A dominant model (GG: 36.81 ± 8.69; GA+AA: 31.58 ± 9.17; P = 0.006) and the AGO2 rs4961280C&gt;A recessive model (CC+CA: 35.42 ± 8.77; AA: 22.00 ± 4.24; P = 0.035) exhibited a significantly decreased number of CD4+ helper T cells. Our study showed that AGO1 and AGO2 polymorphisms are associated with the prevalence of RIF. Hence, the results suggest that variations in AGO1 and AGO2 genotypes may be useful clinical biomarkers for the development and prognosis of RIF.
Harnessing targeted DNA methylation and demethylation using dCas9
Authors: Pflueger Christian , Swain Tessa , Lister Ryan 
Published: November 2019

Abstract
DNA methylation is an essential DNA modification that plays a crucial role in genome regulation during differentiation and development, and is disrupted in a range of disease states. The recent development of CRISPR/catalytically dead CRISPR/Cas9 (dCas9)-based targeted DNA methylation editing tools has enabled new insights into the roles and functional relevance of this modification, including its importance at regulatory regions and the role of aberrant methylation in various diseases. However, while these tools are advancing our ability to understand and manipulate this regulatory layer of the genome, they still possess a variety of limitations in efficacy, implementation, and targeting specificity. Effective targeted DNA methylation editing will continue to advance our fundamental understanding of the role of this modification in different genomic and cellular contexts, and further improvements may enable more accurate disease modeling and possible future treatments. In this review, we discuss strategies, considerations, and future directions for targeted DNA methylation editing.
Time‐domain single‐ended fault location method that does not need remote‐end system information
Authors: Zhang Chenhao , Song Guobing , Yang Liming , Sun Zhongyu 
Published: November 2019

This study presents a single‐ended fault location method that does not need the information of the remote‐end system. As the foundation of the proposed method, the fault location equations under different fault types are derived based on the fault loops. The fault distance, fault impedance, and equivalent resistance and inductance of the remote‐end system are considered as the unknowns of the fault location equation. Then the equation can be solved using the Gauss‐Newton method, by which the four unknowns can be obtained. Therefore, the proposed method is not sensitive to the fault impedance and the system parameters. A two‐terminal 500 kV ac transmission line test system is built in PSCAD to verify the proposed method. Numerous simulation data of faults of different fault distances, fault impedances, and fault types verify the accuracy and robustness of the proposed method. The proposed method is a time‐domain method that can locate the fault using 5 ms data after the faults.
Exploitation of MOSFET‐based AC switches in capacitive impedance matching networks in inductive wireless power transfer systems
Authors: Alexandru Cristina A. , Zhu Dibin 
Published: November 2019

This study investigates the exploitation of MOSFET‐based AC switches in capacitive impedance matching networks (IMNs) for inductive wireless power transfer (WPT). The IMN optimum capacitance has been chosen for a 200 kHz resonant frequency. The activation of the tuning capacitor on the tuning branch is achieved with the use of MOSFET AC switches in order for the WPT system to achieve maximum power transfer efficiency. The MOSFET AC switch is modelled as an internal parasitic resistor and capacitor connected in parallel. A WPT analytical model is developed to study the effects of the MOSFET's parasitic elements on the WPT system's efficiency and is verified experimentally. Various MOSFET switches and relays have been implemented as the IMN switching elements and compared when tested under the same conditions. It is concluded that MOSFET AC switches which have low on‐resistances and small parasitic capacitances are desirable as they have a smaller impact on efficiency. Additionally, parasitic capacitances of MOSFET AC switches need careful consideration for different resonant frequencies as they can affect the overall IMN tuning capacitance especially when they are turned off. Comparing to commonly used relay switches, MOSFET‐based AC switches have similar switching performance but are significantly smaller in size.
Space vector modulation based switching schemes for high frequency link matrix rectifier in discontinuous conduction mode
Authors: Lan Dongdong , Kumar Sahoo Sanjib , Srinivasan Dipti 
Published: November 2019

A high‐frequency link matrix rectifier with a pure capacitive output filter has the advantages of reduced converter size and clamped voltage over the rectifier diode. However, when the converter operates in discontinuous conduction mode (DCM), there is high input current distortion. The distortion has to be compensated such that the total harmonic distortion (THD) does not exceed 5%. The straightforward way of reducing distortion is by designing the switching scheme. In this study, switching schemes based on space vector modulation have been studied to improve the input current quality in DCM. Several existing switching schemes and newly proposed switching schemes have been analysed and compared. A 600 W laboratory prototype has been built to verify the effect of switching schemes over input current quality. The experimental results show that the input current THD can be greatly reduced by applying proper switching scheme.
Coupled inductor‐based DC–DC converter with high voltage conversion ratio and smooth input current
Authors: Samuel Vijay Joseph , Keerthi Gna , Mahalingam Prabhakar 
Published: November 2019

In this study, a coupled inductor (CI)‐based high step‐up DC–DC converter is presented. The proposed topology is developed from a primitive quadratic boost converter (QBC) structure. A two‐phase interleaved QBC structure is obtained by employing multi‐winding CIs instead of discrete inductors as the energy storage magnetic element. The voltage gain is further extended by using (i) voltage lift capacitor, (ii) CIs and (iii) voltage multiplier cells. Consequently, the voltage stress on the main switches is reduced to 18.9% of the output voltage. Moreover, interleaving mechanism helps in achieving a smooth input current profile; the input current ripple is only 4.59% of the total input current magnitude. To validate the proposed concept, 18 V/380 V, 150 W prototype converter is constructed and experimented. Under experimentation, the converter delivers the required power to the load at 95% full‐load efficiency. Further, under closed‐loop condition, the output voltage from the converter is regulated to the required standard value of 380 V DC.
Investigation on space charge and charge trap characteristics of Al–epoxy nanocomposites
Authors: Chillu Naresh , Jayaganthan Rengaswamy , Nageshwar Rao Burjupati , Danikas Michael , Tanaka Toshikatsu , Sarathi Ramanujam 
Published: November 2019

Optimised quantity of nano‐aluminium (Al) filler mixed with epoxy resin using percolation threshold criteria was adapted to fabricate nanocomposite with desired space‐charge and charge‐trap properties. Surface potential variation due to charge deposition on to the insulating material under transient voltages was investigated, and it showed the improved decay rate with reduced trap depth for the nanocomposites with nanofillers addition in the range between 0.5 and 5 wt%. Threshold electric field calculated based on space‐charge density variation by pulsed electroacoustic measurement was found to be between 7–9 kV/mm and maximum electric field calculated through polarity reversal test is about 9 kV/mm, for 5 wt% (for the optimised value of nanofiller) epoxy nano‐Al composites.
Immunohistochemical expression of P53, Ki-67, and CD34 in psoriasis and psoriasiform dermatitis
Authors: Ramezani Mazaher , Shamshiri Atefeh , Zavattaro Elisa , Khazaei Sedigheh , Rezaei Mansour , Mahmoodi Rozhano , Sadeghi Masoud 
Published: November 2019

Background: Psoriasis is the prime example of psoriasiform tissue pattern and should be differentiated from other psoriasiform dermatoses both clinically and histopathologically.
Aim: To evaluate immunohistochemical expression of P53, Ki-67, and CD34 in psoriasis and psoriasiform dermatitis for diagnostic purposes.
Methods: An analytical cross-sectional study was performed on the paraffin blocks of 60 psoriasis and 31 psoriasiform dermatitis patients between 2014 and 2017. The selected formalin-fixed paraffin-embedded tissues from each biopsy specimen were cut into 4-micron sections. Initial sections were stained by hematoxylin and eosin staining. Primary antihuman antibodies against P53, Ki-67, and CD34 were applied. Positive control samples for biomarkers were received from former strongly positive samples of papillary endothelial hyperplasia, high grade lymphoma, and breast ductal carcinoma for CD34, Ki-67, and P53, respectively.
Results: Out of 60 psoriasis patients, 56.7% were men, with the mean age of 36.8 years. From 31 psoriasiform patients, 45.2% were men, with the mean age of 37.5 years. Both groups were matched in terms of sex and age. The mean staining of three markers was more significant in psoriasiform dermatitis than psoriasis.
Conclusion: In spite of some other researches, the present study showed expression of P53, Ki-67, and CD34 biomarkers were significantly higher in psoriasiform dermatitis than psoriasis.
Stationäre berufsdermatologische Prävention im Institut für interdisziplinäre dermatologische Prävention und Rehabilitation (iDerm) an der Universität Osnabrück
Authors: Skudlik C. , John S. M. 
Published: November 2019

ZusammenfassungDas im Jahr 2004 eingeführte „Verfahren Haut“ der Deutschen Gesetzlichen Unfallversicherung (DGUV) sieht bei schweren, ambulant therapieresistenten berufsbedingten Hauterkrankungen vor, im Rahmen eines gestuft gegliederten Präventionskonzeptes eine Individualpräventionsmaßnahme durchzuführen, die sich aus einem dreiwöchigen stationären Aufenthalt mit einem interdisziplinären medizinischen und pädagogischen Interventionsprogramm und einer engmaschigen nachstationären ambulanten hautärztlichen Betreuung zusammensetzt. Diese Maßnahme (Tertiäre Individual-Prävention, TIP) wurde u. a. maßgeblich im Institut für interdisziplinäre Dermatologische Prävention und Rehabilitation (iDerm) an der Universität Osnabrück konzipiert und evaluiert und stellt hier einen Schwerpunkt des täglichen Wirkens dar. Mittels Langzeitevaluation der Verläufe nach TIP über einen Zeitraum von 5 Jahren konnte gezeigt werden, dass das Maßnahmenprogramm effektiv ist und der deutlichen Mehrheit der Teilnehmer erlaubt, trotz ursprünglich schwerer Berufsdermatose die berufliche Tätigkeit dauerhaft auszuüben bei gleichzeitig langfristig signifikanter Besserung des Hautbefundes und der Lebensqualität. Die im iDerm an der Universität Osnabrück angebotene stationäre Rehabilitationsmaßnahme TIP steht allen Versicherten der Unfallversicherungsträger in der Bundesrepublik Deutschland zur Verfügung; die Indikation hierfür kann problemlos durch die behandelnden Hautärztinnen und Hautärzte im Hautarztberichtsformular gestellt werden.
Homöopathische Palliation bei einem Schäferhund mit Neurofibrom
Authors: Danowski Klaus 
Published: November 2019

ZusammenfassungEs wird über einen Fall von inkompletter Radialislähmung durch ein Neurofibrom bei einer 10-jährigen Schäferhündin berichtet. Eine chirurgische Lösung war nach Aussage der vorbehandelnden Klinik-Tierärzte nicht ohne Zerstörung des Nervens möglich. Unter der Medikation mit Luesinum und Calcium arsenicosum entwickelt sich der Fall innerhalb von 4 Monaten bis zur Beschwerdefreiheit zurück. Die anschließende 3-monatige Unterbrechung der Behandlung führte zur Entwicklung einer Herzinsuffizienz und letztlich infaust verlaufenden Meningitis.
Intracranial nonvestibular neurinomas: Young neurosurgeons’ experience
Authors: Chowdhury Forhad Hossain , Haque Mohammod R. , Kawsar Khandkar A. , Sarker Mainul H. , Hasan Mahmudul , Goel Atul H. 
Published: November 2019

ABSTRACT
Background and Objectives: Neurinoma arising from other than nonvestibular cranial nerves is less prevalent. Here we present our experiences regarding the clinical profile, investigations, microneurosurgical management, and the outcome of nonvestibular cranial nerve neurinomas. Materials and Methods: From January 2005 to December 2011, the recorded documents of operated nonvestibular intracranial neurinomas were retrospectively studied for clinical profile, investigations, microneurosurgical management, complications, follow-up, and outcomes. Results: The average follow-up was 24.5 months. Total number of cases was 30, with age ranging from 9 to 60 years. Sixteen cases were males and 14 were females. Nonvestibular cranial nerve schwannomas most commonly originated from trigeminal nerve followed by glossopharyngeal+/vagus nerve. There were three abducent nerve schwannomas that are very rare. There was no trochlear nerve schwannoma. Two glossopharyngeal+/vagus nerve schwannomas extended into the neck through jugular foramen and one extended into the upper cervical spinal canal. Involved nerve dysfunction was a common clinical feature except in trigeminal neurinomas where facial pain was a common feature. Aiming for no new neurodeficit, total resection of the tumor was done in 24 cases, and near-total resection or gross total resection or subtotal resection was done in 6 cases. Preoperative symptoms improved or disappeared in 25 cases. New persistent deficit occurred in 3 cases. Two patients died postoperatively. There was no recurrence of tumor till the last follow-up. Conclusion: Nonvestibular schwannomas are far less common, but curable benign lesions. Surgical approach to the skull base and craniovertebral junction is a often complex and lengthy procedure associated with chances of significant morbidity. But early diagnosis, proper investigations, and evaluation, along with appropriate decision making and surgical planning with microsurgical techniques are the essential factors that can result in optimum outcome.
Obstructive sleep apnea screening by heart rate variability-based apnea/normal respiration discriminant model
Authors: Nakayama Chikao , Fujiwara Koichi , Sumi Yukiyoshi , Matsuo Masahiro , Kano Manabu , Kadotani Hiroshi 
Published: November 2019

Abstract

Objective: Obstructive sleep apnea (OSA) is a common sleep disorder; however, most patients are undiagnosed and untreated because it is difficult for patients themselves to notice OSA in daily living. Polysomnography (PSG), which is the gold standard test for sleep disorder diagnosis, cannot be performed in many hospitals. This fact motivates us to develop a simple system for screening OSA at home. Approach: The autonomic nervous system changes during apnea, and such changes affect heart rate variability (HRV). This work develops a new apnea screening method based on HRV analysis and machine learning technologies. An apnea/normal respiration (A/N) discriminant model is built for respiration condition estimation for every heart rate measurement, and an apnea/sleep ratio is introduced for final diagnosis. A random forest is adopted for the A/N discriminant model construction, which is trained with the PhysioNet apnea-ECG database. Main results: The screening performance of the proposed method was evaluated by applying it to clinical PSG data. Sensitivity and specificity achieved 76% and 92%, respectively, which are comparable to existing portable sleep monitoring devices used in sleep laboratories. Significance: Since the proposed OSA screening method can be used more easily than existing devices, it will contribute to OSA treatment.
Predictors Associated with the Curricular Performance of Student-Athletes
Authors: Dominado Noel L. 
Published: November 2019

Abstract
This study was conducted to establish the potential association between predictors and curricular performance of student-athletes. To accomplish the reason for the study the descriptive research design was used. One hundred thirty-four athletes from the Province of Pampanga who competed in the Central Luzon Regional Association (CLRAA) in the School Year 2016-2017 served as respondents of the study. The sports involvement of student-athletes and its perceived effects on their curricular performance. For physical aspect, sixty-six or 49.25% agreed is the poor balance diet, Seven or 5.52% strongly disagreed that the injury acquired from sports involvement affected their curricular performance. On the other hand, the social aspect, Seventy-four or 55.22% agreed is teachers’ discrimination was a factor that affects their curricular performance. While 11 or 8.20% strongly disagreed is the discrimination from classmate can effect on their curricular performance. Likewise, in the emotional aspect, seventy or 52.23% disagreed Failure/defeat in sports involvement may result in loss of Self-reliance. While six or 4.47% strongly agreed that defeats led to the loss of Courage. Lastly, mental aspect, Fifty-nine or 44.02% agreed that cause of anger often becomes the focus of attention. While eleven or 8.20% which were describing strongly agreed is the worry.