bluuebunny/crossref_metadata_2025_split huggingface dataset:
Characterisation of Cardiac Health in the Reduced Uterine Perfusion Pressure Model and a 3D Cardiac Spheroid Model, of Preeclampsia
Authors: Richards Claire , Sesperez Kimberly , Chhor Michael , Ghorbanpour Sahar , Rennie Claire , Ming Clara Liu Chung , Evenhuis Chris , Nikolic Valentina N , Orlic Natasa Karadzov , Mikovic Zeljko , Stefanovic Milan , Cakic Zoran , McGrath Kristine , Gentile Carmine , Bubb Kristen , McClements Lana 
Published: December 2020

Abstract
Background: Preeclampsia is a life-threatening cardiovascular disorder of pregnancy that leads to an increased risk of ongoing cardiovascular and metabolic disorders. Much of the pathogenesis and mechanisms involved in cardiac health are unknown. A novel anti-angiogenic protein, FKBPL, is emerging as having a potential role in both preeclampsia and cardiovascular disease (CVD). Therefore, in this study we aimed to investigate the role of FKBPL in cardiac health in the rat reduced uterine perfusion pressure (RUPP) model and 3D cardiac spheroid model, of preeclampsia.Methods: The RUPP model was induced in pregnant rats and histological analysis performed on the heart, kidneys, liver and placenta (n≥6). Picrosirius red staining was performed to quantify collagen I/III deposition in rat hearts, placentae and livers as an indicator of fibrosis. RT-qPCR was used to determine changes in Fkbpl, Icam1, Vcam1, Flt1 and/or Vegfa mRNA in hearts and/or placentae and ELISA was used to evaluate cardiac brain natriuretic peptide (BNP45) and FKBPL secretion in rat hearts. Cardiac spheroids were generated using human cardiac fibroblasts (HCFs) and human coronary artery endothelial cells (HCAECs) and treated with patient plasma from normotensive controls, early-onset preeclampsia (EOPE) and late-onset preeclampsia (LOPE); (n=3). FKBPL and CD31 expression was quantified by immunofluorescent labelling.Results: The RUPP procedure induced significant increase in blood pressure (p<0.001), cardiac collagen deposition (p<0.001) and cardiac BNP45 (p<0.05). It also induced a significant increase in cardiac FKBPL mRNA expression (p<0.05) and protein levels (p<0.01). RUPP placentae also exhibited increased collagen deposition and decreased Flt1 mRNA expression (p<0.05). RUPP kidneys revealed an increase in average glomerular size (p<0.05). Cardiac spheroids showed a significant increase in FKBPL expression when treated with LOPE patient plasma (p<0.05) and a trend towards increased FKBPL expression following treatment with EOPE plasma (p=0.06).Conclusions: The rat RUPP model induced cardiac, renal and placental features reflective of preeclampsia in humans. FKBPL was increased in the hearts of RUPP rats and in cardiac spheroids treated with plasma from women with preeclampsia, reflective of restricted angiogenesis in this disorder. Elucidation of this novel FKBPL mechanism in cardiac health in preeclampsia could be key in preventing future CVD.
Cost-effectiveness Analysis of Neoadjuvant Versus Adjuvant Chemotherapy for cT2-4N0-1 Non-small Cell Lung Cancer Patients During Initial Treatment Phase
Authors: Wu Dongdong , Li Juan , Wang Yubo , Huang Hao , Chunji Huang 
Published: December 2020

Abstract
Objective: The choice between neoadjuvant chemotherapy (NAC) and adjuvant chemotherapy (AC) remains controversial in the treatment of non-small cell lung cancer (NSCLC). There is no significant difference in NAC and AC’s effectiveness. We investigate the cost-effectiveness of NAC versus AC for NSCLC.Method: A decision tree model was designed from a payer perspective to compare NAC and AC treatments for NSCLC. Parameters included overall survival (OS), surgical complications, chemotherapy adverse event (AE), treatment initiation probability, treatment time frame, treatment cost, and quality of life (QOL). Sensitivity analyses were performed to characterize model uncertainty in the base cases.Result: With the same overall survival, AC treatment strategy produces a cost saving of ￥618.90 and an incremental quality-adjusted life-years (QALY) of 0.10 years per patient. If median OS of NAC is 1.4 months more than AC, NAC would be cost effective (CE) at a ￥35446/QALY threshold. The model was robust enough to handle variations to all input parameters except the overall survival. In the probability sensitivity analysis, AC remained dominant in 54.6% of simulations.Conclusion: The modeled cost effectiveness analysis indicates that with operable NSCLC, AC treatment is more cost effective compared to NAC. If NAC provides a longer survival advantage, this treatment strategy may be cost-effective. The OS is the main factor that influences cost-effectiveness analysis.
Ground Water Induced Changes in Velocities of P and S Waves (Vp and Vs) Measured Using Accurately Controlled Seismic Source
Authors: Suzuki Rina , Yamaoka Koshun , Tsuji Shuhei , Watanabe Toshiki 
Published: December 2020

Abstract
We analyzed the temporal variation in the travel times of both the P and S waves (Vp and Vs) for 14 months at Toyohashi (central Japan) with a continuously operating vibration source that could produce both P and S waves efficiently. The seismic waves produced by the source, which is named ACROSS (accurately-controlled routinely-operated signal system), were recorded by three nearby seismic stations, and the travel time variation at each station was calculated using the transfer function calculated from the recorded data. We observed the seasonal variations in the Vp and Vs for all the stations—which can be interpreted using the change in the fluid saturation and crack density of subsurface rocks—are consistent with the variation in the ground water level. The short-term responses to rainfall are observed at the nearest station; the interpretation of the changes in crack density and saturation is inconsistent with the ground water observation partly due the initial response to rainfall. This can be interpreted as an air-water mixture within pores or cracks on a fine scale.
Curcumin and Its Analog Alleviate Diabetes-induced Damages by Regulating Inflammation and Oxidative Stress in Brain of Diabetic Rats​
Authors: Miao Chengfeng , Chen Hanbin , Li Yulian , Guo Ying , Xu Feifei , Chen Qi , Zhang Yanyan , Hu Mengjun , Chen Guorong 
Published: December 2020

Abstract
Background: Diabetic encephalopathy is a severe diabetes complication with cognitive dysfunction and neuropsychiatric disability. The mechanisms underlying diabetic encephalopathy is believed to be relevant with oxidative stress, vascular amylin deposition, immune receptors, inflammation, etc. This study wanted to evaluate the ability of curcumin and its analog A13 to alleviate oxidative stress and inflammation in diabetes-induced damages in brain.Methods: Sixty adult male Sprague-Dawley rats were divided into 5 groups: normal control (NC) group, diabetes mellitus (DM) group, curcumin-treated diabetes mellitus (CUR) group, high dose of A13-treated diabetes mellitus (HA) group, low dose of A13-treated diabetes mellitus (LA) group. Activation of the nuclear factor kappa-B (NF-κB p65) pathway was detected by RT-qPCR, immunohistochemical (IHC) staining and Western blot; oxidative stress was detected by biochemical detection kit; brain tissue sections were stained with hematoxylin–eosin (HE) staining and Myelin staining. Results: RT-qPCR, IHC staining and Western blot showed that curcumin and A13 treatment could inhibit the NF-κB p65 pathway. Curcumin and A13 increased the activity of superoxide dismutase and decreased the malondialdehyde level in the brain of diabetic rats. Furthermore, HE staining and Myelin staining demonstrated that the histological lesions of the brain in diabetic rats could be significantly ameliorated by curcumin and A13.Conclusion: Curcumin analog A13 could alleviate the damages in the brain of diabetes rats by regulating the pathways of inflammation and oxidative stress. A13 may be a new potential therapeutic agent for diabetic encephalopathy.
The Expression of miR-513c and miR-3163 was Downregulated in Tumor Tissues Compared with Margin Tissues of Patients with Breast Cancer
Authors: Delgir Soheila , Ilkhani Khandan , Safi Asma , Montazari Vahid , Seif Farhad , Bastami Milad , Alivand Mohammad-Reza 
Published: December 2020

Abstract
Background: Breast cancer (BC) is the most common invasive cancer with different subtypes that its metabolism is unique compared with normal cells. Glutamine is considered critical nutrition, that many cancer cells, particularly BC cells are dependent on it for growth and proliferation. Therefore, targeting glutamine metabolism, especially enzymes related to this pathway, can be beneficial to design anti-cancer agents. Recently, evidence has shown that miRNAs with a short length and single-strand properties play a significant role in regulating the genes related to glutamine metabolism and may control the development of cancer. Methods: In-silico analysis confirmed that miR-513c and miR-3163 might be involved in glutamine metabolism, the expression level of these two miRNAs was examined in eighty BC tissues and margin tissues. Furthermore, in-silico analysis was applied to predict the potential biological processes and molecular pathways of miR-513c and miR-3163 based on their gene targets. Results: In-silico studies revealed the top categories of biological processes and cellular pathways that play a critical role in metabolism reprogramming and cancer development were target genes for miR-513c and miR-3163. The current study showed that miR-513c (p-value= 0.02062 and fold change= -2.3801) and miR-3163 (p-value= 0.02034 and fold change= -2.3792) were downregulated in tumor tissues compared to margin tissues. Furthermore, the subgroup analysis did not show any substantial relationship between expression levels of these two miRNAs and factors such as age, family history cancer, and abortion. Conclusion: miR-513c and miR-3163 are downregulated in BC tissues, which may act as tumor suppressors and may also be considered as potential therapeutic target for patients with BC.
The Impact of Preoperative Biliary Drainage on Postoperative Outcomes after Pancreaticoduodenectomy: A Single Center Retrospective Study
Authors: Mo Daihai , Ma Hongyun , Wang Zhen , Hu Jiayang , Li Gang 
Published: December 2020

Abstract
Background The effect of preoperative biliary drainage (PBD) on the short-term outcomes after pancreaticoduodenectomy (PD) remains controversial. Methods 164 consecutive patients with obstructive jaundice who underwent PD in our center from 2016 to 2017 were retrospectively analyzed. The 120 patients who underwent PBD prior to PD (PBD group) were compared with 44 patients who did not (DS group). The short-term outcomes include overall morbidity, severe complications, postoperative pancreatic fistula(POPF), postpancreatectomy hemorrhage(PPH), intra-abdominal abscess (IAA), sepsis, delayed gastric emptying (DGE), postoperative hospital stay and 90-day death were assesed. Results There were no significant statistical differences in overall morbidity, severe complications, POPF, PPH, IAA, LOS and ninety-day mortality between two groups. The incidence of DGE in PBD group was significantly lower than that in DS group (4.5% vs. 19.2%, P = 0.021). Multivariate regression analysis showed that age over 66 years (OR: 3.094,95% CI: 1.268-7.55) and direct surgery (OR: 5.298, 95% CI: 1.176-23.865) were independent risk factors for DGE. Conclusion For patients with obstructive jaundice, preoperative biliary drainage is independently associated with delayed gastric emptying, but does not affect the overall morbidity and mortality of patients undergoing PD.
Effect of Huangqin Decoction on Regulating Intestinal Flora in Colitis Mice Characterized as The Inhibition of NOD2-Dependent Pathway
Authors: Huang Shaowei , He Jinrong , Chen Yanping , Wang Xiaojing , Li Yanyang , Su Yulin , Wen Ruyan , Li Xiuling , Yang Guanghua , Luo Shuang , Zhou Lian , Luo Xia 
Published: December 2020

Abstract
Background: Dysfunction of the intestinal flora is an important factor in the occurrence and development of ulcerative colitis (UC). Chinese herb Huangqin decoction (HQD) can regulate intestinal flora in UC mice, yet its specific mechanism remains unclear. Our study aimed to clarify the mechanism of HQD in regulating intestinal flora of UC mice.Methods: 3%DSS - induced colitis mice were used to explore the pharmacodynamics of HQD. Colonic bacterial infiltration and the changes of intestinal flora were detected by FISH and 16SrDNA. Expression of RegⅢγ and PRRs (NOD2, TLR5, TLR4) were detected by FCM and WB, respectively. In addition, WB, SqPCR, or IHC were used for the expression of Nod2, MyD88, Rip2, and NF-κB p65 in colon. Cytokines were determined by ELISA.Results: HQD significantly restored the weight, the colonic length, and peripheral blood cell counts of colitis mice; reduced the DAI score and the histological score. 16SrDNA identification showed HQD regulated the balance of intestinal flora. Moreover, the expression of RegⅢγ was suppressed and colonic bacterial infiltration was prevented by HQD. Furthermore, the results of WB showed expression of NOD2 and TLR4 was inhibited by HQD, especially NOD2. And the data of WB, SqPCR and IHC explained that the NOD2-dependent pathway was inhibited by HQD.Conclusions: Our results reveal the mechanism of HQD(1g/kg) in regulating the intestinal flora of colitis mice, which may be closely characterized as the inhibition of the activation of Pattern Recognition Receptors (PRRs), especially NOD2.
The Cognitive Walkthough for Implementation Strategies (CWIS): A Pragmatic Method for Assessing Implementation Strategy Usability
Authors: Lyon Aaron R , Coifman Jessica , Cook Heather , McRee Erin , Liu Freda F. , Ludwig Kristy , Dorsey Shannon , Koerner Kelly , Munson Sean A. , McCauley Elizabeth 
Published: December 2020

Abstract
Background. Implementation strategies have flourished over the last decade in an effort to increase integration of research evidence into clinical practice. Most strategies are complex, socially-mediated processes. Many are complicated, expensive, and ultimately impractical to deliver in real-world settings. The field lacks methods to assess the extent to which implementation strategies are usable and aligned with the needs and constraints of the individuals and contexts who will deliver or receive them. Drawn from the field of human-centered design, cognitive walkthroughs are an efficient assessment method with potential to surface aspects of strategies that may inhibit their usability and, ultimately, their effectiveness. This article presents a novel cognitive walkthrough methodology for evaluating strategy usability as well as an example application to a post-training consultation strategy to support mental health clinicians in the education sector to adopt measurement-based care.Method. The Cognitive Walkthrough for Implementation Strategies (CWIS) is a pragmatic, mixed-methods approach for evaluating complex, socially-mediated implementation strategies in health. CWIS includes six steps: (1) determine preconditions; (2) hierarchical task analysis; (3) task prioritization; (4) convert tasks to scenarios; (5) pragmatic group testing; and (6) usability issue identification, classification, and prioritization. A facilitator conducted two group testing sessions with clinician users (N = 10), guiding participants through 6 scenarios and 11 associated subtasks. Clinicians reported their anticipated likelihood of completing each subtask and provided qualitative justifications during group discussion. Following the walkthrough sessions, users completed a quantitative assessment of strategy usability.Results. Average subtask success ratings indicated substantial variability across participants and subtasks. Usability ratings (scale: 0-100) of the consultation protocol averaged 71.3 (SD = 10.6). Twenty-one usability problems were identified via qualitative coding and classified by severity and problem type to explain the ratings. High-severity problems included potential misalignment between consultation and clinical service timelines as well as digressions during consultation processes.Conclusions. Ratings indicated that usability of the consultation protocol was at the low end of the “acceptable” range. Collectively, the 21 usability issues explained the ISUS quantitative usability data and provided specific direction for usability enhancements. The current study provides preliminary evidence for the utility of CWIS to assess strategy usability and generate a blueprint for redesign.
Improving Implementation of Smoking Cessation Guidelines in Pregnancy Care: Development of an Intervention to Address System, Maternity Service Leader and Clinician Factors
Authors: Passey Megan , Adams Catherine , Paul Christine , Atkins Lou , Longman Jo M. 
Published: December 2020

Abstract
BackgroundSmoking during pregnancy increases the risk of multiple serious adverse infant, child and maternal outcomes, yet nearly 10% of Australian women still smoke during pregnancy. Despite evidence-based guidelines that recommend routine and repeated smoking cessation support (SCS) for all pregnant women, the provision of recommended SCS remains poor. Guidance on developing complex interventions to improve health care recommends drawing on existing theories, reviewing evidence, undertaking primary data collection, attending to future real-world implementation, and designing and refining interventions using iterative cycles with stakeholder input throughout. Here we describe using the Behaviour Change Wheel (BCW) to apply these principles in developing an intervention to improve provision of SCS in Australian maternity services.MethodsWorking closely with key stakeholders in the New South Wales (NSW) health system, we applied the steps of the BCW method then undertook a small feasibility study in one service to further refine the intervention. Stakeholders were engaged in multiple ways – as a core research team member, through a project Advisory Group, targeted meetings with policy makers, a large workshop to review potential components and the feasibility study. ResultsBarriers to and enablers of providing SCS were identified in five of six components described in the BCW method (psychological capability, physical opportunity, social opportunity, and in reflective and automatic motivation). These were mapped to intervention types and we selected education, training, enablement, environmental restructuring, persuasion, incentivisation and modelling as suitable in our context. Through application of the APEASE criteria (Affordability, Practicability, Effectiveness, Acceptability, Side effects, and Equity) in the stakeholder workshop, behaviour change techniques were selected and applied in developing the intervention which includes systems, clinician and leadership elements. The feasibility study confirmed feasibility and acceptability of the midwifery component and the need to further strengthen the leadership component. ConclusionsUsing the BCW method combined with strong stakeholder engagement from inception resulted in transparent development of the MOHMQuit intervention, which targets identified barriers to and enablers of the provision of SCS and is developed specifically for the context in which it will be implemented. The intervention is being trialed in eight public maternity services in NSW.
Readiness to Implement Symptom Management Care Pathways in Pediatric Cancer
Authors: Dupuis L Lee , Grimes Allison , Vettese Emily , Klesges Lisa M. , Sung Lillian 
Published: December 2020

Abstract
Background: To describe barriers to pediatric cancer symptom management care pathway implementation and the impact of the COVID-19 pandemic on clinical research evaluating their implementation. Methods: We included 25 pediatric oncology hospitals in the United States that supported a grant submission to perform a cluster randomized trial in which the intervention encompassed care pathways for symptom management. A survey was distributed to site principal investigators to measure contextual elements related to care pathway implementation. Questions included the inner setting measures of the Consolidated Framework for Implementation Research (CFIR), study-specific potential barriers and the impact of the COVID-19 pandemic on clinical research. The Wilcoxon rank sum test was used to compare characteristics of institutions that agreed that their department supported the implementation of symptom management care pathways vs. institutions that did not agree.Results: Of the 25 sites, one withdrew because of resource constraints and one did not respond, leaving 23 institutions. Among the seven CFIR constructs, the least supported was implementation climate; 57% agreed there was support, 39% agreed there was recognition and 39% agreed there was prioritization for symptom management care pathway implementation at their institution. Most common barriers were lack of person-time to create care pathways and champion their use (35%), lack of interest from physicians (30%) and lack of information technology resources (26%). Most sites reported no negative impact of the COVID-19 pandemic across research activities. Sites with fewer pediatric cancer patients were more likely to agree that staff are supported to implement symptom management care pathways (P=0.003). Conclusions: The most commonly reported barriers to implementation were lack of support, recognition and prioritization. The COVID-19 pandemic was not a major barrier to clinical research activities in pediatric oncology.Clinical Trial Registration: NCT04614662
Gender Difference in the Association Between Serum Uric Acid Level and Metabolic Syndrome and Components: A Cross-Sectional Analysis among Chinese Young Adults
Authors: Fang Zhengmei , Chen Yan , Zhu Lijun , He Lianping , Jin Yuelong , Yao Yingshui 
Published: December 2020

Abstract
Background: This study aimed to investigate the associations between serum uric acid (SUA) levels and metabolic syndrome (MetS) and MetS components in Chinese young adults. Methods: A cross-sectional survey was conducted among 3044 young adults (1266 men and 1778 women). The anthropometric index, lipid profile, fasting blood glucose and SUA levels were measured. Male and female participants were grouped according to the quartiles of SUA level separately. Multiple logistic regression analyses were performed to evaluate the association of SUA quartiles with MetS and its components. Results: The overall prevalence of hyperuricemia and MetS was 29.7% and 2%, respectively. Multiple logistic regression analysis revealed that compared with the lowest 2 quartiles of SUA together, the highest quartile showed an association with the prevalence of MetS and high triglyceride in males, and OR (95% CI) were 3.438(1.090-10.841) and 4.364(2.133-8.930) respectively after adjustments confounding factors. In terms of abdominal obesity, compared with the lowest 2 quartiles of SUA together, the OR (95% CI) was 1.976(1.128-3.459) for those in the third quartile and 1.766(1.020-3.057) for those in the highest quartile after adjustments confounding factors in females. Conclusions: This study suggested a significant positive relationship between SUA and MetS and its components among young adults. Hence, routine measurement of SUA is recommended to prevent hyperuricemia and its related complications.