bluuebunny/crossref_metadata_2025_split huggingface dataset:
Combining cellular immunotherapy was an optional choice for unresectable advanced HCC: a systematic review and meta-analysis
Authors: Wang Jing , Feng Lingxin , Zhang Linwei 
Published: March 2020

Abstract
Background The efficacy of cellular immunotherapy in advanced hepatocellular carcinoma (HCC) was controversial. This study was conducted to compare the effectiveness of combining cellular immunotherapy with that of incurable treatment alone. Methods The Ovid Medline, Embase, Cochrane Library and Pubmed were systematically searched due to January 8th 2020. The keywords include “immunotherapy”, “HCC” and study type. Treatment response was evaluated and progression-free survival (PFS) and overall survival (OS) were calculated using hazard ratio (HR) and 95% confidence interval (CI). Results A total of 19 studies with 1,275 patients were included in the meta-analysis. The median complete response rate (CR) was 19% in combining cellular immunotherapy comparing to 9% in the control group (RR=0.55, P=0.003). No significant difference was found in partial response and stable disease (RR=1.06 and 0.78, P>0.05, respectively). The progression disease rate was higher in the non-cellular immunotherapy group (31%) compared to the cellular immunotherapy group (RR=2.20, P=0.002). Patients treating with cellular immunotherapy had a better OS and PFS compared to those without cellular immunotherapy (HR=0.52 and 0.63, P<0.001). In the subgroup analysis, the only CIK infusion therapy and combined DC with CIK perfusion therapy patients had a better OS (HR=0.52 and 0.49, P<0.001 and P=0.002, respectively). Conclusion Our results suggested that combining use of cellular immunotherapy in advanced HCC could increase the complete response rate, and thereafter extend the progression-free and overall survival rate. Subgroup analysis suggested that combining use of CIK and DC or using CIK alone could provide the benefit in survival outcome.
FKBP-related ncRNA-mRNA axis in breast cancer
Authors: Xiong Hanchu , Chen Zihan , Zheng Wenwen , Sun Jing , Fu Qingshuang , Teng Rongyue , Chen Jida , Xie Shuduo , Wang Linbo , Yu Xiao-Fang , Zhou Jichun 
Published: March 2020

Abstract
Background Breast cancer (BC) is a disease with morbidity ranking the first of women worldwidely. FK506-binding protein (FKBP) family has been demonstrated to possess various functions by interacting with different molecular targets in BC. However, a comprehensive ncRNA-mRNA regulatory axis of FKBP has not yet been reported. Methods FKBP related miRNAs were obtained from miRWalk database. Then, potential lncRNAs, transcription factors as well as mRNAs of screened differentially expressed miRNAs (DE-miRNAs) were analysed by using LncBase v.2, miRGen v3 and miRWalk database. Additionally, differential expression and prognostic analysis of lncRNAs were evaluated using TANRIC database. Next, GO annotation and KEGG pathway analysis were processed using DAVID database. Protein-Protein Interaction (PPI) network was established and hub genes were identified using STRING database. Finally, differential expression and prognostic analysis of hub genes were further conducted using UALCAN and bc-GenExMiner v4.2 database, respectively. Results Eleven DE-miRNAs, consisting of four FKBP4 related DE-miRNAs and seven FKBP5 related DE-miRNAs, were screened. 482 predicted lncRNAs were found for DE-miRNAs. Then, expression and prognostic results of nine of top twenty lncRNAs of BC were significantly identified. LINC00662 and LINC00963 expression were significantly associated with patients’ overall survival (OS). Then, nine potential upstream transcription factors were identified in motifs of DE-miRNAs. 320 target genes were identified for GO annotation and KEGG pathway analysis, which were mainly enriched in cysteine-type endopeptidase activity involved in apoptotic process. Construction and analysis in PPI network showed that RAB7A was selected as a hub gene with the toppest connectivity scores. Differential expression analysis of nine in top ten hub genes of BC were significantly identified. RAB7A and ARRB1 expression were significantly related with BC patients’ OS. Conclusions In current study, we firstly established a predicted FKBP-related ncRNA-mRNA regulatory network, thus exploring a comprehensive interpretation of molecular mechanisms and providing potential clues in seeking novel therapeutics for BC. In the future, much more experiments should be conducted to verify our findings.
High level of resistance in the mosquito Anopheles arabiensis to pyrethroid insecticides from low malaria transmission zone of Moroto district, Karamoja region, Uganda: Implication for malaria vector control
Authors: Echodu Richard , Anena Juliet , Iwiru Tereza , Mireji Paul , Malinga Geoffrey Maxwell , Opiyo Elizabeth A. , Iga Julius , David Onanyang 
Published: March 2020

Abstract
Background Karamoja region of Uganda previously classified as low malaria transmission zone is currently experiencing significant upsurge of malaria incidences. Long lasting insecticidal nets (LLINs) impregnated with pyrethroids constitute a major tool for malaria control in this region. Efficacy of this tool can be hampered by resistance to the pyrethroids in the Anopheles mosquito vectors. Resistance status of these mosquitoes in this region is poorly understood, effectively hampering better understanding of the impact of LLINs in the malaria control initiative. Here, we assessed susceptibility of the Anopheles arabiensis from the region to deltamethrin, permethrin (pyrethroids) and pirirmiphos-methyl (organophosphate) insecticides. Method We collected anopheline mosquito larvae from their natural habitats and reared them to adult emergence in situ field insectary in Karamoja region. We then identified them morphological to species level and exposed 513 emerge adult female An gambiae s.l., mosquitoes to diagnostic dosages of deltamethrin (0.05%), permethrin (0.75%) and pirimiphos-methyl (0.25%) pyrethroids exposure using the standard WHO insecticide susceptibility test assay. Synergic assays using piperonyl butoxide (PBO) were done to check for the involvement of detoxification enzymes in pyrethroid resistant populations. We then screened for knockdown resistance (KDR) and mosquito species diversity using Polymerase Chain Reaction (PCR). Results Majority (96%) of the mosquitoes we sampled were identified as An. arabiensis and 4% as An. gambiae sensu stricto . We observed cross-resistance to both deltamethrin (11.9%) and permethrin (47%) but susceptibility (100% mortality) to pirimiphos-methyl in An. arabiensis . The pre-exposure to PBO ameliorated the resistance to both pyrethroids. We detected homozygous KDR -eastern variant in 1.8 and 50% of the An. arabiensis and An. gambiae s.s. respectively. Conclusion Anopheles arabiensis and An. gambiae s.s. are the malaria vector in Karamoja region with An. arabiensis predominating. Both species are susceptible to pirimiphos-methyl but resistant to both deltamethrin and permethrin, through a metabolic process (phenotype). Mosquotoes with genetic (kdr) mutations for resistance were minimal and hence have minimal contribution to the pyrethroid resistance profile. An. arabiensis can thus be controled in Karamoja region using deltamethrin and/or permethrin impregnated mosquito nets integrated with PBO and/or through indoor residual spraying of sprayable human dwellings with pirimiphos-methyl.
A survey on health care data using Machine learning Algorithms
Authors: B Manjulatha , Pabboju Suresh 
Published: March 2020

Abstract
Big data in the healthcare has been growing rapidly in recent years. A massive amount of patient’s data is generated through various sources in this digitalized environment including wearable devices, mobile devices, electronic health records and medical centres. The medical data, which are exploding exponentially includes a large volume of structured and unstructured data. Patients information is shared and managed by many sources such as insurance, in the form of prescription, healthcare providers including the attackers which causes a severe security threats their confidential data. Cybercriminals use a variety of social engineering attacks like phishing, leakage of data, embedding virus and trojan horse into the attachments, DNS snooping, sniffing, and many more. In this paper a brief overview is done on different data mining and security techniques which are implemented in healthcare data. The study and its presentation in this context would be helpful in not only analysing but also predicting chronic diseases depending upon the available stored data which has been collected from various sources in the area of medical expert system.
The role of the globular heads of the C1q receptor in TcdA-induced human colonic epithelial cell apoptosis via a mitochondria-dependent pathway
Authors: Liang Jinhua , Ning Yongzhong , Dong Li , Li Qi , Chen Ling , Xu Yanmin 
Published: March 2020

Abstract
Background: Clostridioides (formerly Clostridium) difficile infection is the leading cause of antibiotic-associated colitis. Studies have demonstrated that C. difficile toxin A (TcdA) can cause apoptosis in many human cell types. The purpose of this study was to investigate the relationships among exposure to TcdA, the role of globular heads receptor of C1q (gC1qR) gene and the underlying intracellular apoptotic mechanism of human colonic epithelial cells (NCM 460).Methods: In this study, gC1qR expression was examined using real-time polymerase chain reaction (PCR), western blot and immunohistochemical staining analysis. Cells viability was assessed by the water-soluble tetrazolium salt (WST-1) assay, apoptosis of cells was assessed by flow cytometry and terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling (TUNEL) Assay. Mitochondrial function was assessed via reactive oxygen species (ROS) generation, changes in the mitochondrial membrane potential (ΔΨm) and the content of ATP.Results: Our study demonstrated that the concentration of TcdA increasing from 10 ng/ml to 20 ng/ml inhibited cell viability and induced cell apoptosis ( p < 0.01). Moreover, TcdA induced gC1qR mRNA and protein expression. Overexpression of gC1qR could cause the mitochondrial dysfunction (including production of ROS, decrease of ΔΨm and the content of ATP) and cell apoptosis. However, silencing of gC1qR gene could reverse the TcdA-induced cell apoptosis and mitochondrial dysfunction.Conclusion: Therefore, these data support a mechanism wherein gC1qR plays a crucial role in TcdA-induced human colonic epithelial cells apoptosis involving a mitochondrial dependent manner.
Clinical implications of the American Joint Committee on Cancer (AJCC) 8th edition update in seminoma pT1 subclassification
Authors: Fontes-Sousa Mário , Lobo João , Magalhães Helena , Cassis João , Malheiro Mariana , Ramos Sância , Henrique Rui , Martins Ana , Maurício Maria Joaquina 
Published: March 2020

Abstract
Background Seminoma accounts for 30-50% of Testicular Germ-Cell Tumors (TGCT) - the most common solid malignancy in men aged 15-35 years. The American Joint Committee on Cancer (AJCC) 8th edition (2018) created the subclassifications pT1a (tumor size < 3 cm) and pT1b (≥ 3 cm), despite not universally recognized. The authors propose to further understand its potential impact in clinical practice.Methods: All consecutive cases of seminoma stage I pT1 treated at two institutions between January2005 and December 2016. Clinical data were retrieved, and variables were analyzed using SPSS. Reviewof relevant literature on the topic.Discussion: Seminoma pT1 was identified in 58 patients. By using newly AJCC criteria, 29 (50.0%) would have been pT1a and 29 (50.0%) pT1b. Median follow- up time 5.8 years. Three recurrences wererecorded (2 in pT1a and 1 in pT1b, all under surveillance protocol); no deaths occurred. Rete testis invasion (RTI) and extensive necrosis (EN) were associated with pT1b (P<0.0001 and P=0.023, respectively), therefore pT1b was associated with RTI and EN, known adverse biological features, but the clinical impact could not to be assessed with the present methodology. Our results suggest an excellent prognosis, regardless of subcategorization, thus a larger population and a longer follow-up time areneeded to understand the impact of the updated criteria.Conclusions: In our population, the retrospective analysis of the newly created AJCC criteria showed no significant difference in recurrence or death, although pT1b was associated to adverse biological markers. We would recommend using the AJCC staging system, although the individual risk of relapse, long-term toxicities and patient preferences should be taken into account when considering surveillance or active treatment options.
Effect of Hookah (water pipe) smoking on Anti-Mullerian Hormone levels
Authors: Albeitawi Soha , Almehaisen Lama , Obeidat Rawan , Shehab Qasem , Elisabetta Garavaglia 
Published: March 2020

Abstract
Purpose: Hookah (water pipe) smoking, an ancient method of tobacco smoking, is regaining widespread popularity all over the world. Although research in this field is still lacking, its harmful effect on human health through the increased risk of cardiovascular disease, infection, and cancer has been proven. Cigarette smoking has been found to have a detrimental effect on reproductive function. However, no data about the effect of hookah smoking on human fertility or reproductive outcomes are available. In this study, we aimed to determine if smoking hookah has a harmful effect on human ovarian reserve, anti-Mullerian hormone levels, and cycle regularity. Methods: A total of 100 healthy women within the 25-35 years age range undergoing laboratory investigations were randomly chosen to participate. After obtaining consent, they filled a questionnaire about smoking habits and menstrual data. Blood samples were withdrawn for anti-Mullerian hormone testing. The anti-Mullerian hormone levels of 50 women smoking hookah but not cigarettes were compared to 50 non-smoking women. Collected data were analyzed by analysis of variance and independent samples T-test. Results: There were no significant differences in mean anti-Mullerian hormone levels and cycle regularity between hookah smokers and non-smokers. Conclusion: Hookah smoking has no significant effect on anti-Mullerian hormone levels.
Live birth rates in different subgroups of poor ovarian responders according to Bologna criteria and POSEIDON group classification
Authors: Mashayekhy Mehri , Barabi Forouzan , Arabipoor Arezoo , Zolfaghari Zahra 
Published: March 2020

Abstract
Background Recently, some studies evaluated the live births rates (LBRs) in two poor ovarian responders (POR) classification methods separately, but present study was designed to compare the LBRs among different groups of patients on the basis of Bologna criteria or POSIEDON group classifications and find the important predictive factors for LBR in patients with POR. Methods In this cross-sectional study, the database containing clinical and laboratory information on infertility treatment cycles were evaluated from December 2015 to December 2017 and all the patients with at least one POR after standard controlled ovarian stimulation (COH) during IVF/ICSI cycles were evaluated. The eligible patients were enrolled and divided into five and four groups according to the Bologna criteria and POSEIDON groups’ classifications, respectively. Results 812 patients with POR diagnosis were investigated which in overall 517 of them had embryo transfer in the last treatment cycle (63.6%) and total clinical pregnancy rate 19.3% (100 cases) and live birth rates 16.1% (86 cases) were found. When the patients were grouped on the Basis of the POSEIDON classification, the highest LBR was observed in the POSEIDON group III. On the other hand, according to the Bologna criteria, 41 patients were not included in any group, and in the analysis of cycle outcome, the LBR in the Bologna group II was more than other groups. The multivariable regression analysis revealed that the significant independent variables remained in the model as important predictive factor for live births in the study population were the number and quality of the transferred embryos transmission (good and excellent quality), and the POSSIDON classification group (POSSIDON group III). Conclusions On the basis of present results, the POSEIDON group classification could be more comprehensive and practical than Bologna criteria for diagnosing and categorizing POR patients. In addition, it was found the number and quality of transferred embryos were the most important prognostic factor for live birth in POR patients; therefore we suggest to consider COH protocols with a freezing embryos strategy, and the collection of more good quality embryos to improve the probability of a live birth.
Flood Realities, Development Faults and Perceptions – Natural and Anthropogenic causes of 2014 Flood in Kashmir
Authors: MALIK ISHFAQ HUSSAIN , Hashmi S Najmul Islam 
Published: March 2020

Abstract
The regularity of risk in modern societies permeates everyday life— from the air that we breathe, to the food that we eat, to the water we drink and to where we work and live. We live in a globalized world associated with risk societies which is caused by a myriad of global processes. These processes interact to produce an unforeseen dangers and endless array of risks. Kashmir being politically and ecologically fragile area needs a special understanding in terms of its physical and political geography. Kashmir suffered the worst flood in last 109 years in the year 2014. The present research analyses the disaster management in Jammu and Kashmir with special emphasis on causes of 2014 flood in Kashmir Valley. It digs deeper into the politics of urbanization and how the state has turned a blind eye to lake encroachment and land grab. The flood occurred due to the mismanagement of the resources and the faulty means of development. Increased level of urbanisation and its unplanned growth, and encroachment on the banks of Jhelum River proved to be detrimental to the environment and people. The other factors like deforestation, glacial melt and destruction of wetlands in the fragile Himalayan ecosystem particularly in the Western Himalayas, lack of proper disaster management plan in the valley, climate change, land grab, extensive catchment of Jhelum River, administrative laxity, lack of proper flood channel for Jhelum River etc. corroborated in the occurrence of the flood. The combination of these factors increased the vulnerability of the people to the 2014 flood in Kashmir Valley. Natural calamities are known to change politics, and ﬂoods are a great leveller. The political instability and unsettled conditions in Kashmir compound problems many times over.
Antioxidant dressing therapy versus standard wound care in chronic wounds (REOX study): Study protocol for a randomized controlled trial
Authors: Comino-Sanz Inés María , López-Franco María Dolores , Castro Begoña , Pancorbo-Hidalgo Pedro Luis 
Published: March 2020

Abstract
Background: A wound that does not heal in the orderly stages of the healing process or does not heal within three months is considered a chronic wound. Wound healing is impaired when the wound remains in the inflammatory stage for too long. A range of factors can delay the healing process: imbalance between proteases and protease inhibitors in the wound bed; bacterial colonization and the presence of biofilm; and oxidative stress.Recently, wound management has improved significantly. A new antioxidant dressing has been developed, which combines an absorbent matrix obtained from Locust Bean Gum (LBG) galactomannan and a hydration solution with curcumin and N-acetylcysteine (NAC). This dressing combines the advantages of moist healing in exudate management and free radical neutralization, achieving wound reactivation.The primary aim of this study is to compare the effect of antioxidant dressing on chronic wound healing with the use of standard wound dressing in patients with hard-to-heal wounds.Methods: We will conduct a multicentre, open, randomized, controlled trial with parallel groups. Participants will be selected from three primary public health-care centres, located in Andalucía (southern Spain). Patients will be randomized into an intervention group (antioxidant dressing) or control group (standard wound dressing). Assessment will be carried out in weeks 2, 4, 6 and 8. The follow-up of patients will be prolonged until week 8 or complete healing.Discussion: The findings from this study should provide scientific evidence on the efficacy of 20 the antioxidant dressing as an alternative for the treatment of chronic wounds. This study fills 21 some of the gaps in the existing knowledge about patients with hard-to-heal wounds.
Distribution of prostheses position after primary total knee arthroplasty on dominant and non-dominant sides
Authors: Fengchao ZHAO , Lei LIU , Guochun Zha , Guanjie Yang , Shizhuang Xu , Li Zheng , Dadi Li 
Published: March 2020

Abstract
Background To investigate the changes of prosthesis position after primary total knee arthroplasty (TKA) on the dominant and non-dominant sides. Methods A retrospective analysis was made of 132 patients (148 knees) who underwent primary TKA from December 2016 to December 2018 in our hospital. The patients were divided into dominant group (right side) and non-dominant group (left side) according to the position of the three right-handed surgeons. Total operation time, posterior distal femur angle (PDFA), posterior proximal tibia angle (PPTA), malposition rate of femoral prostheses in sagittal, postoperative hip-knee-ankle angle (HKA), mechanical lateral distal femoral angle (mLDFA), mechanical medial proximal tibial angle (mMPTA) were compared between the two groups. Results The mean total operation time was in dominant group was 111.8(111.8±26.9) and in non-dominant group was 113.7(113.7±26.1) (P>0.05). Postoperative sagittal alignment, the mean PDFA was 90.2(90.2°±3.3°) in dominant group and 88.8(88.8°±3.6°) in non-dominant group (P<0.05). The mean PPTA was 84.8(84.8°±2.8°) in dominant group and 85.4(85.4°±3.7°) in non-dominant group (P>0.05). The incidence of malposition of sagittal femoral prosthesis in dominant group was 6/76 (7.9%) and that in non-dominant was 15/72 (20.8%). There was a significant difference between the two groups (χ2=5.083，P=0.024). Postoperative coronal alignment, the mean angle of components placement was 2.1°±4.2° for HKA, 88.7°±2.7° for mLDFA, 89.5°±2.7° for mMPTA in dominant group and 1.6°±4.1° for HKA, 89.0°±2.8° for mLDFA, 89.9°±2.7° for mMPTA in non-dominant group, respectively. There were no significant difference between the two groups (t=0.804 P=0.423, t=-0.564 P= 0.574, t=-1.011 P= 0.314). Conclusion Our study suggests that surgeon handedness is likely to be one of factors that affect the sagittal placement of femoral component. Orthopaedic surgeons should be aware of the potential problems and consider taking extra precautions to mitigate the risk before performing surgery.