bluuebunny/crossref_metadata_2025_split huggingface dataset:
Characteristics of two myoviruses induced from the coastal photoheterotrophic bacterium Porphyrobacter sp. YT40
Authors: Lin Wenxin , Chen Qi , Liu Yanting , Jiao Nianzhi , Zheng Qiang 
Published: January 2020

ABSTRACT
In this study, we characterized two induced myoviruses from one marine photoheterotrophic bacterium Porphyrobacter sp. YT40 belonging to the Sphingomonadales family in Alphaproteobacteria. The genome sequence of prophage A is ∼36.9 kb with an average GC content of 67.1%, and its core or functional genes are homologous to Mu or Mu-like phages. Furthermore, induced viral particles from prophage A show a knob-like neck structure, which is only found in bacteriophage Mu. The genome size of prophage B is ∼36.8 kb with an average GC content of 65.3%. Prophage B contains a conserved gene cluster Q-P-O-N-M-L, which is unique in P2 phages. Induced viral particles from prophage B display an icosahedral head with a diameter of ∼55 nm and a 130 ± 5 nm long contractile tail. To our knowledge, this is the first report that characterizes the induced P2-like phage in marine Alphaproteobacteria. Phylogeny analyses suggest that these two types of prophages are commonly found in sequenced bacteria of the Sphingomonadales family. This study sheds light on the ongoing interaction between marine bacteria and phages, and improves our understanding of bacterial genomic plasticity and evolution.
Comparative Genomics Identifies Putative Signatures of Sociality in Spiders
Authors: Tong Chao , Najm Gabriella M , Pinter-Wollman Noa , Pruitt Jonathan N , Linksvayer Timothy A 
Published: January 2020

Abstract
Comparative genomics has begun to elucidate the genomic basis of social life in insects, but insight into the genomic basis of spider sociality has lagged behind. To begin, to characterize genomic signatures associated with the evolution of social life in spiders, we performed one of the first spider comparative genomics studies including five solitary species and two social species, representing two independent origins of sociality in the genus Stegodyphus. We found that the two social spider species had a large expansion of gene families associated with transport and metabolic processes and an elevated genome-wide rate of molecular evolution compared with the five solitary spider species. Genes that were rapidly evolving in the two social species relative to the five solitary species were enriched for transport, behavior, and immune functions, whereas genes that were rapidly evolving in the solitary species were enriched for energy metabolism processes. Most rapidly evolving genes in the social species Stegodyphus dumicola were broadly expressed across four tissues and enriched for transport functions, but 12 rapidly evolving genes showed brain-specific expression and were enriched for social behavioral processes. Altogether, our study identifies putative genomic signatures and potential candidate genes associated with spider sociality. These results indicate that future spider comparative genomic studies, including broader sampling and additional independent origins of sociality, can further clarify the genomic causes and consequences of social life.
Socioeconomic Inequalities in Disability-free Life Expectancy in Older People from England and the United States: A Cross-national Population-Based Study
Authors: Zaninotto Paola , Batty George David , Stenholm Sari , Kawachi Ichiro , Hyde Martin , Goldberg Marcel , Westerlund Hugo , Vahtera Jussi , Head Jenny 
Published: January 2020

Abstract

Background
We examined socioeconomic inequalities in disability-free life expectancy in older men and women from England and the United States and explored whether people in England can expect to live longer and healthier lives than those in the United States.

Methods
We used harmonized data from the Gateway to Global Aging Data on 14,803 individuals aged 50+ from the U.S. Health and Retirement Study (HRS) and 10,754 from the English Longitudinal Study of Ageing (ELSA). Disability was measured in terms of impaired activities and instrumental activities of daily living. We used discrete-time multistate life table models to estimate total life expectancy and life expectancy free of disability.

Results
Socioeconomic inequalities in disability-free life expectancy were of a similar magnitude (in absolute terms) in England and the United States. The socioeconomic disadvantage in disability-free life expectancy was largest for wealth, in both countries: people in the poorest group could expect to live seven to nine fewer years without disability than those in the richest group at the age of 50.

Conclusions
Inequalities in healthy life expectancy exist in both countries and are of similar magnitude. In both countries, efforts in reducing health inequalities should target people from disadvantaged socioeconomic groups.
Propagation of distinct Love-wave pulses from regional to teleseismic distances in continental and oceanic environments
Authors: Furumura Takashi , Kennett Brian L N 
Published: January 2020

SUMMARYSurface waves are usually dispersive with long wave trains and steady decay of amplitude with distance. However, if the group velocity is nearly constant for a span of periods a strong pulse is produced that retains its amplitude for large distances. This situation arises for the fundamental mode of Love waves in the period band 40–500 s for crust and mantle structures with a positive gradient of S wave speed in the uppermost mantle. Such a distinct Love-wave pulse with limited dispersion observed at teleseismic distance is termed the G wave in honour of Gutenberg. The long-period G-wave pulse caused by large earthquakes carries a large amount of energy to substantial distances, with significant effects across the globe, for example event triggering. A similar G-type Love-wave pulse with a much shorter-period of 10–20 s is generated for crustal structures without thick sediment. Such pulses produce anomalously large ground displacement at near-regional distances with, for example an overestimate of surface wave magnitude. We investigate the generation and propagation mechanism of the G-type Love-wave pulses in the crust and upper-mantle with the analysis of observed strong motion records from the Mw 6.2 2016 Central Tottori earthquake and the Mw 9.0 2011 Off Tohoku earthquake in Japan, in conjunction with 3-D finite-difference simulation of seismic wave propagation and analysis of dispersion curves.
Overabundance of unhealthy food advertising targeted to children on Guatemalan television
Authors: Cosenza-Quintana Emma Lucia , Morales-Juárez Analí , Ramirez-Zea Manuel , Vandevijvere Stefanie , Kroker-Lobos Maria F 
Published: January 2020

Abstract
To assess, for the first time, the extent (by hour channel) and nature (e.g. persuasive marketing techniques (PMT) and health-related claims) of unhealthy food advertisements (ads) targeted at children (3–11 years) on the six most-watched television (TV) channels in Guatemala. We recorded 864 h of video on the six most popular channels featuring children’s programmes. We classified food and beverage ads as permitted or non-permitted for marketing to children, according to the 2015 World Health Organisation (WHO) nutrient profile. Furthermore, we also analysed PMT (i.e. premium offers, promotional characters, brand benefit claims) and health-related claims. Most food ads (85%) were non-permitted to be marketed to children. Non-permitted food ads were six times more likely, either on weekdays or weekends, for all programme and channel categories compared with permitted food ads. There was no difference in the frequency of non-permitted food ads between peak and non-peak hours, weekend and weekdays or children and non-children programmes. PMT and health-related claims were present in all food ads (5.3 ± 1.9 techniques/claims per ad). There is a need to regulate food ads on TV channels featuring children’s programmes in Guatemala as a result of a high frequency of non-permitted food ads and extensive use of PMT together with health-related claims.
Structural basis for the dominant or recessive character of GLIALCAM mutations found in leukodystrophies
Authors: Elorza-Vidal Xabier , Xicoy-Espaulella Efren , Pla-Casillanis Adrià , Alonso-Gardón Marta , Gaitán-Peñas Héctor , Engel-Pizcueta Carolyn , Fernández-Recio Juan , Estévez Raúl 
Published: January 2020

Abstract
Megalencephalic leukoencephalopathy with subcortical cysts (MLC) is a type of leukodystrophy characterized by white matter edema, and it is caused mainly by recessive mutations in MLC1 and GLIALCAM genes. These variants are called MLC1 and MLC2A with both types of patients sharing the same clinical phenotype. In addition, dominant mutations in GLIALCAM have also been identified in a subtype of MLC patients with a remitting phenotype. This variant has been named MLC2B. GLIALCAM encodes for an adhesion protein containing two immunoglobulin (Ig) domains and it is needed for MLC1 targeting to astrocyte–astrocyte junctions. Most mutations identified in GLIALCAM abolish GlialCAM targeting to junctions. However, it is unclear why some mutations behave as recessive or dominant. Here, we used a combination of biochemistry methods with a new developed anti-GlialCAM nanobody, double-mutants and cysteine cross-links experiments, together with computer docking, to create a structural model of GlialCAM homo-interactions. Using this model, we suggest that dominant mutations affect different GlialCAM–GlialCAM interacting surfaces in the first Ig domain, which can occur between GlialCAM molecules present in the same cell (cis) or present in neighbouring cells (trans). Our results provide a framework that can be used to understand the molecular basis of pathogenesis of all identified GLIALCAM mutations.
Long-term outcomes after coronary artery bypass surgery in patients with diabetes
Authors: Axelsson Tomas Andri , Adalsteinsson Jonas A , Arnadottir Linda O , Helgason Dadi , Johannesdottir Hera , Helgadottir Solveig , Orrason Andri Wilberg , Andersen Karl , Gudbjartsson Tomas 
Published: January 2020

Abstract

OBJECTIVES
Our aim was to investigate the outcome of patients with diabetes undergoing coronary artery bypass grafting (CABG) surgery in a whole population with main focus on long-term mortality and complications.

METHODS
This was a nationwide retrospective analysis of all patients who underwent isolated primary CABG in Iceland between 2001 and 2016. Overall survival together with the composite end point of major adverse cardiac and cerebrovascular events was compared between patients with diabetes and patients without diabetes during a median follow-up of 8.5 years. Multivariable regression analyses were used to evaluate the impact of diabetes on both short- and long-term outcomes.

RESULTS
Of a total of 2060 patients, 356 (17%) patients had diabetes. Patients with diabetes had a higher body mass index (29.9 vs 27.9 kg/m2) and more often had hypertension (83% vs 62%) and chronic kidney disease (estimated glomerular filtration rate ≤60 ml/min/1.73 m2, 21% vs 14%). Patients with diabetes had an increased risk of operative mortality [odds ratio 2.52, 95% confidence interval (CI) 1.27–4.80] when adjusted for confounders. 5-Year overall survival (85% vs 91%, P &lt; 0.001) and 5-year freedom from major adverse cardiac and cerebrovascular events were also inferior for patients with diabetes (77% vs 82%, P &lt; 0.001). Cox regression analysis adjusting for potential confounders showed that the diagnosis of diabetes significantly predicted all-cause mortality [hazard ratio (HR) 1.87, 95% CI 1.53–2.29] and increased risk of major adverse cardiac and cerebrovascular events (HR 1.47, 95% CI 1.23–1.75).

CONCLUSIONS
Patients with diabetes have significantly lower survival after CABG, both within 30 days and during long-term follow-up.
Development of a practical prediction score for acute renal injury after surgery for Stanford type A aortic dissection
Authors: Dong Ning , Piao Hulin , Du Yu , Li Bo , Xu Jian , Wei Shibo , Liu Kexiang 
Published: January 2020

AbstractOBJECTIVESAcute kidney injury (AKI) is a common complication of cardiovascular surgery that is associated with increased mortality, especially after surgeries involving the aorta. Early detection and prevention of AKI in patients with aortic dissection may help improve outcomes. The objective of this study was to develop a practical prediction score for AKI after surgery for Stanford type A acute aortic dissection (TAAAD).METHODSThis was a retrospective cohort study that included 2 independent hospitals. A larger cohort of 326 patients from The Second Hospital of Jilin University was used to identify the risk factors for AKI and to develop a risk score. The derived risk score was externally validated in a separate cohort of 102 patients from the other hospital.RESULTSThe scoring system included the following variables: (i) age &gt;45 years; (ii) body mass index &gt;25 kg/m2; (iii) white blood cell count &gt;13.5 × 109/l; and (iv) lowest perioperative haemoglobin &lt;100 g/l, cardiopulmonary bypass duration &gt;150 min and renal malperfusion. On receiver operating characteristic curve analysis, the score predicted AKI with fair accuracy in both the derivation [area under the curve 0.778, 95% confidence interval (CI) 0.726–0.83] and the validation (area under the curve 0.747, 95% CI 0.657–0.838) cohorts.CONCLUSIONSWe developed a convenient scoring system to identify patients at high risk of developing AKI after surgery for TAAAD. This scoring system may help identify patients who require more intensive postoperative management and facilitate appropriate interventions to prevent AKI and improve patient outcomes.
The role of nanotechnology on post-combustion CO2 absorption in process industries
Authors: Kumar Ravinder , Mangalapuri Rajesh , Ahmadi Mohammad Hossein , Vo Dai-Viet N , Solanki Rajniesh , Kumar Pawan 
Published: January 2020

Abstract
At present, higher greenhouse gas (GHG) have triggered global efforts to reduce their level as much as possible for sustainable development. Carbon dioxide is one of the imperative anthropogenic emissions due to its increased excessive accumulation in the environment. Thus, serious attention is required to reduce the level of CO2 using advanced and efficient CO2 capture technologies. Carbon dioxide capture and storage (CCS) technologies may play an important role in this direction. At present, solvent-based sorbents are being utilized in CO2 capture for various industrial processes. In this category, the characters of non-materials are playing a crucial role to improve the CO2 absorption capacity of the process. This study is mainly focused on the role of nanotechnology in the post-combustion CO2 absorption process. The functions of nanomaterials and nanoparticles have been studied in the present work. Additionally, various challenges related to absorption efficiency using nanomaterials have been discussed. The study concludes that the higher thermal stability and exceptional properties of nanomaterials popularized them for use in CO2 capture processes.
Analysis of limited-memory BFGS on a class of nonsmooth convex functions
Authors: Asl Azam , Overton Michael L 
Published: January 2020

Abstract
The limited-memory BFGS (Broyden-Fletcher-Goldfarb-Shanno) method is widely used for large-scale unconstrained optimization, but its behavior on nonsmooth problems has received little attention. L-BFGS (limited memory BFGS) can be used with or without ‘scaling’; the use of scaling is normally recommended. A simple special case, when just one BFGS update is stored and used at every iteration, is sometimes also known as memoryless BFGS. We analyze memoryless BFGS with scaling, using any Armijo–Wolfe line search, on the function $f(x) = a|x^{(1)}| + \sum _{i=2}^{n} x^{(i)}$, initiated at any point $x_0$ with $x_0^{(1)}\not = 0$. We show that if $a\ge 2\sqrt{n-1}$, the absolute value of the normalized search direction generated by this method converges to a constant vector, and if, in addition, $a$ is larger than a quantity that depends on the Armijo parameter, then the iterates converge to a nonoptimal point $\bar x$ with $\bar x^{(1)}=0$, although $f$ is unbounded below. As we showed in previous work, the gradient method with any Armijo–Wolfe line search also fails on the same function if $a\geq \sqrt{n-1}$ and $a$ is larger than another quantity depending on the Armijo parameter, but scaled memoryless BFGS fails under a weaker condition relating $a$ to the Armijo parameter than that implying failure of the gradient method. Furthermore, in sharp contrast to the gradient method, if a specific standard Armijo–Wolfe bracketing line search is used, scaled memoryless BFGS fails when $a\ge 2 \sqrt{n-1}$regardless of the Armijo parameter. Finally, numerical experiments indicate that the results may extend to scaled L-BFGS with any fixed number of updates $m$, and to more general piecewise linear functions.
Phospholipase A2 from bee venom increases poly(I:C)-induced activation in human keratinocytes
Authors: Nakashima Akina , Tomono Susumu , Yamazaki Tatsuya , Inui Masanori , Morita Naoko , Ichimonji Isao , Takagi Hidekazu , Nagaoka Fumiaki , Matsumoto Misako , Ito Yasuhiko , Yanagishita Takeshi , Miyake Kensuke , Watanabe Daisuke , Akashi-Takamura Sachiko 
Published: January 2020

Abstract
Bee venom (BV) induces skin inflammation, characterized by erythema, blisters, edemas, pain and itching. Although BV has been found to have an inhibitory effect on toll-like receptors (TLRs), we here show that BV enhances keratinocyte responses to polyinosinic-polycytidylic acid [poly(I:C)], a ligand for TLR3. Our results revealed that the enhanced TLR activity was primarily induced by secretory phospholipase A2 (sPLA2), a component of BV (BV-sPLA2). PLA2 mediates the hydrolysis of membrane phospholipids into lysophospholipids and free fatty acids. We demonstrated that BV-sPLA2 increased the intracellular uptake of poly(I:C), phosphorylation of the nuclear factor-kappa B (NF-κB) and mitogen-activated protein kinases (MAPKs), and poly(I:C)-mediated interleukin 8 production in human keratinocytes. We further showed that the enzymatic activity of BV-sPLA2 was essential for the increased uptake of poly(I:C). These findings suggest that BV-sPLA2 may induce a modification of the cell membrane structure, leading to enhanced poly(I:C) uptake in keratinocytes. BV-sPLA2 might be able to promote wound healing by enhancing TLR3 responses.
Overexpression of RSK4 reverses doxorubicin resistance in human breast cancer cells via PI3K/AKT signalling pathway
Authors: Mei Yan , Liao Xiaoming , Zhu Lingyu , Yang Huawei 
Published: January 2020

Abstract
Doxorubicin (DOX) is one of the most effective chemotherapy drugs for the treatment of metastatic breast cancer (BC), but drug resistance becomes an obstacle to treatment. This study aims to investigate the role of Ribosomal S6 protein kinase 4 (RSK4) in regulating BC resistance to DOX. We first used Kaplan–Meier Plotter to identify the prognostic roles of RSK4 in BC. DOX-resistant BC cells (MCF-7/DOX) were constructed and the expression of RSK4 was determined by reverse transcript polymerase chain reaction and western blot. Subsequently, we overexpressed the RSK4 in MCF-7/DOX cells, and measured drug resistance, colony formation, cell migration, invasion ability and cell apoptosis after transfection. In addition, western blot was used to explore the expression of apoptosis-related proteins and BC-resistance protein. Effects of RSK4 on activation of the PI3K/AKT signalling pathway were also tested. Furthermore, tumour xenograft in nude mice was constructed to observe the effect of RSK4 overexpression on tumour growth in vivo. In conclusion, RSK4 was positively correlated with survival rate in BC patients, which is lowly expressed in MCF-7/DOX. Meanwhile, the overexpression of RSK4 may inhibit drug resistance, cell migration, invasion, apoptosis and tumour growth. RSK4 may effectively attenuate DOX resistance in BC by inhibiting the PI3K/AKT signalling pathway.
For the Benefit of Students: Memory and Anatomical Learning at Bologna in the Fourteenth to Early Sixteenth Centuries
Authors: Robison Kira L 
Published: January 2020

Abstract
The anatomical textbook in the late Middle Ages was one part of a greater pedagogical process that involved students’ seeing, hearing, reading, and eventually knowing information about the human body. By examining the role of the anatomical textbook and accompanying bodily images in anatomical learning, this article illuminates the complexity and self-consciousness of anatomical education in the medieval university, as professors focused on ways to enhance student memory of the material. Traditionally, the history of anatomy has been heavily influenced by the anatomical Renaissance of the late-sixteenth century, highlighting a focus on innovative medical knowledge and the scientific method. However, if we engage a pedagogical lens when looking at these medieval authors, it becomes quickly obvious that the whole point of university medicine was not to explore unknown boundaries and discover new ideas of medicine, but rather to communicate the current and established body of knowledge to those not familiar with it.
Evolving pulsation of the slowly rotating magnetic β Cep star ξ1 CMa
Authors: Wade G A , Pigulski A , Begy S , Shultz M , Handler G , Sikora J , Neilson H , Cugier H , Erba C , Moffat A F J , Pablo B , Popowicz A , Weiss W , Zwintz K 
Published: January 2020

ABSTRACT
Recent BRITE-Constellation space photometry of the slowly rotating, magnetic β Cep pulsator ξ1 CMa permits a new analysis of its pulsation properties. Analysis of the two-colour BRITE data reveals the well-known single pulsation period of 0.209 d, along with its first and second harmonics. A similar analysis of SMEI and TESS observations yields compatible results, with the higher precision TESS observations also revealing several low-amplitude modes with frequencies below 5 d−1; some of these are likely g modes. The phase lag between photometric and radial velocity maxima – equal to 0.334 cycles – is significantly larger than the typical value of 1/4 observed in other large-amplitude β Cep stars. The phase lag, as well as the strong dependence of phase of maximum light on wavelength, can be reconciled with seismic models only if the dominant mode is the fundamental radial mode. We employ all published photometric and radial velocity measurements, spanning over a century, to evaluate the stability of the pulsation period. The O−C diagram exhibits a clear parabolic shape consistent with a mean rate of period change $\dot{P}=0.34\pm 0.02$ s cen−1. The residuals from the best-fitting parabola exhibit scatter that is substantially larger than the uncertainties. In particular, dense sampling obtained during the past ∼20 yr suggests more complex and rapid period variations. Those data cannot be coherently phased with the mean rate of period change, and instead require $\dot{P}\sim 0.9$ s cen−1. We examine the potential contributions of binarity, stellar evolution, and stellar rotation and magnetism to understand the apparent period evolution.