bluuebunny/crossref_metadata_2025_split huggingface dataset:
Awareness and perception of husbands towards obstetric danger signs in northern Ethiopia
Authors: Fitwi Awot , Buruh Gerezgiher , Rejeu Mohammedseid , Amare Mikias , Getie Addisu , Belete Yemisrach , Meikena Henok Kumsa 
Published: February 2020

Abstract
Background The Ethiopian demographic and health survey indicate that maternal mortality in Ethiopia is one of the highest in the world. The national reproductive strategy of Ethiopia has given emphasis to maternal and newborn health so as to reduce the high maternal and neonatal mortality. One of the targets in the strategies is to ensure that 80% of all families recognize at least three danger signs. However, a few researches have been focused on husband’s awareness towards obstetric danger signs. Objective This study was aimed to assess awareness and perception of husbands towards obstetric danger sign and associated factors in northern Ethiopia, 2018 Methods Community based cross sectional study was done from February 2018 to March 2018, in Mekelle city, Tigray region, Ethiopia. Multi stage sampling was used to reach 620 husbands. Data were entered, cleaned using Epi info version 7 and analyzed by SPSS version 22.0. Results are presented by frequencies, percentages, mean and standard deviation. Binary and multivariable logistic analyses were employed to identify variable associated with awareness and perception of obstetrics danger signs. In multivariable logistic analyses variables with P-value < 0.05 were declare statistically significance. Result About 49.7% and 33.4% of husbands had good awareness and high perception on obstetric danger signs respectively. Husbands with daily labor occupation had 80% (AOR=0.2:95%CI; 0.13-0.44) less likely to have good awareness than governmental employee. Husbands with monthly income of 501-1000 birr had 5.8 times (AOR= 5.8, 95% CI: 1.22-27.89) high perception compared to those with monthly income of less than 500 birr. Conclusion one in two and one in three husbands was having good awareness and high perception about obstetric danger sign respectively. Source of information, occupation of husband, income, occupation of mother and education of husband were significantly associated with husband’s awareness on obstetric danger sign. Moreover variables like monthly income, occupation of mother and number of children were significantly associated with of husband’s perception on obstetric danger signs of their wives. Interventions are needed in creating awareness and perception of husbands’ in obstetric danger signs to recognize its complications.
Enhanced production of recombinant proteins in Corynebacterium glutamicum by construction a bicistronic gene expression system
Authors: Sun Manman , Gao Xiong , Zhao Zihao , Li An , Wang Yali , Liu Xiuxia , Yang Yankun , Bai Zhonghu 
Published: February 2020

Abstract
Background: Corynebacterium glutamicum is a traditional food-grade industrial microorganism， in which an efficient endotoxin-free recombinant protein expression factory is under developing in recent years. However, the intrinsic disadvantages of low recombinant protein expression levels need to be solved. Here, according to the bacteria-specific polycistronic feature, trials have been made of inserting a leading peptide upstream of target genes as an expression enhancer, and we found it improving the expression level of proteins under the control of inducible tac promoter in C. glutamicum CGMCC1.15647 . Results: In this research, the Escherichia coli ( E. coli ) tac promoter combined with 24 different fore-cistron sequences were constructed in a bicistronic manner in C. glutamicum. Three strong bicistronic vectors were isolated and exhibited higher strength under different culture conditions. The compatibility of these bicistronic vectors was further validated using six model proteins- aldehyde dehydrogenase (ALDH), alcohol dehydrogenase (ADH), RamA (regulator of acetate metabolism), Bovine interferon-a (BoIFN-a), glycoprotein D protein (gD) of infectious bovine rhinotracheitis virus (IBRV) and procollagen type I N-terminal peptide (PINP). All examined proteins were highly expressed compared with the original vector of tac promoter. Large-scale production of PINP was also performed in fed-batch cultivation, and the highest PINP production level was 1.2 g/L. Conclusions: In this study, we improved the strength of the inducible promoter tac promoter for C. glutamicum by screening and inserting fore-cistron in front of the target genes. Those vectors with bicistronic expression pattern have strong compatibility for expressing various heterogeneous proteins in high level. This new strategy could be used to further improve the performance of inducible promoters, achieving double competence of inducible control and high yield.
Comparative Analysis of Enoxaparin versus Rivaroxaban in the Treatment of Cancer Associated Venous Thromboembolism: Experience from a Tertiary Care Cancer Centre
Authors: Faqah Anadil , Sheikh Hassan , Bakar Muhammad Abu , Tayyaab Fatima , Khawaja Sahrish 
Published: February 2020

Abstract
BACKGROUND VTE in cancer patients is associated with poor prognosis and remains a leading cause of mortality and morbidity. The purpose of this study was to access if rivaroxaban, a oral factor Xa inhibitor would be an appealing alternate choice to treat cVTE compared with LMWH. METHODS We conducted a retrospective review of comparison between the efficacy and safety profile of enoxaparin versus rivaroxaban in the treatment of venothromboembolism (VTE) in 150 patients with cancer after developing a symptomatic DVT or PE. Baseline patient characteristics and laboratory values were assessed in each arm. Primary efficacy outcome was measured by radiographically confirmed VTE recurrence at different intervals. Primary safety outcome was measured by presence of major and minor bleeding using the ISTH scale. RESULTS Our results showed that there was no statistically significant difference between the incidence of VTE recurrence at 6 months between the Enoxaparin and Rivaroxaban arm (10% vs 14.2%, p=0.42). Historically significant risk factors for VTE in cancer patients such as high platelet count, high leukocyte count, low hemoglobin level, high risk gastrointestinal, genitourinary and lung cancers were not found to be independently significantly associated with the risk of VTE recurrence. Primary safety outcome analysis also showed no statistically significant difference in major (11.2% vs 11.4%) and minor (15% vs 10%) bleeding between enoxaparin versus rivaroxaban arm respectively, (p=0.65). CONCLUSION We conclude that there was no significant difference seen between the efficacy and safety profile of enoxaparin and rivaroxaban in our cancer patient population.
Prevalence of Hookah smoking and associated factors among male high school students in Iraq.
Authors: Al-Delaimy Ahmed K. , Al-Ani Waleed AT 
Published: February 2020

Abstract
Background The use of the hookah-smoking device is increasing at a large scale in the Eastern Mediterranean region and reaching to Western countries. Hookah smoke users are exposed to a lot of chemical compounds and several chronic diseases. The purpose of this study was to determine the prevalence of hookah use among male high school students and to study different associated factors linked with hookah smoking.Methods A descriptive cross-sectional study was conducted among students in three high schools in Al-Karkh District, Baghdad. The study period was from Oct. 2017 till Jan. 2019 and included 847 male students. A structured Knowledge Attitude and Practice questionnaire was used to identify their knowledge and use of hookah smoking. Descriptive, chi-square, bivariate and multivariate logistic regression analysis of data was carried out using the available software statistical package of SPSS-25.Results The overall prevalence of hookah smoking among high school male students was high (46%). More than two-thirds (70.6%) of them think that waterpipe smoking is acceptable socially more than cigarette smoking. More than half of the participants (55%) first heard about hookah smoking from friends and friends constitute close to two-thirds (65.2%) of those who smoke hookah around students. Almost half of the respondents think hookah smoking is not encouraged in the faith of Islam (47.3%) and less than a quarter (20.9%) think it's completely forbidden in Islam.Conclusions Hookah smoking is increasing among high school students and becoming a socially acceptable behavior that needs preventive strategies targeting this age group.
Canadian after-school care providers’ perceived role promoting healthy lifestyles: A focused ethnography
Authors: Elias Pierrette H. , Montemurro Genevieve , Sulz Lauren , Torrance Brian , Storey Kate Elizabeth 
Published: February 2020

Abstract
BACKGROUND: After-school care (ASC) programs have garnered interest in recent years as the hours of 3:00–6:00p.m. are an opportune time for children to engage in healthy behaviours, specifically healthy eating (HE) and physical activity (PA). Care providers are major influencers within the ASC setting, impacting HE and PA opportunities for children. However, little is known regarding the role care providers play in health promotion interventions in the ASC setting, specifically those using comprehensive approaches. The purpose of this research was to explore care providers’ perceptions and experiences implementing the ASC health promotion intervention School’s Out…Let’s Move (SOLMo). SOLMo was guided by the evidence-based comprehensive school health (CSH) framework, and included a focus on HE and PA. METHODS: This research was guided by the qualitative method focused ethnography. Semi-structured interviews with care providers (n=13) taking part in SOLMo were conducted to explore their perceptions of the intervention. Participant observation was included as part of data generation to further understand care providers’ roles. RESULTS: Through latent content analysis, five themes emerged: 1) enhanced awareness; 2) improved programming; 3) strong relationships; 4) collaborative approach; and 5) role tension. CONCLUSIONS: As major influencers, care providers’ play a crucial role, and these results will contribute to implementation strategies used to promote healthy lifestyle behaviours for children.
Air Ducted Blade Design for Horizontal Axis Wind Turbines
Authors: Yigit Cemil 
Published: February 2020

Abstract
Background: Medium and small-scale wind turbines with no pitch control are cheaper than their controlled equivalents but are much more affected by aerodynamic stall, and local boundary layer separation occurs on the blade when critical wind speed is exceeded. As a result, they produce relatively low power. In this study, the delay of separation of the boundary layer from the surface was investigated by increasing the kinetic energy of the low-momentum fluid behind the surface utilizing the airflow from the air ducts added on the blade. Methods: To obtain the optimum performance from the blade, a computer-aided optimization study was conducted by taking the slope, diameter, number, and angle of attack parameters of the air ducts. The response surface methodology, a goal-oriented and multi-purpose method, was used as an optimization method to provide the best possible design according to the constraints and targets set for the parameters. Results: Optimum parameter values were determined using computational fluid dynamics analysis, and air ducted blade design was compared with the air duct-free blade design, and as a result, the power coefficient obtained from the blade was improved between 3.4% and 4.4% depending on wind speed. Conclusions: Opening air ducts up to a critical number of ducts increase viscous forces. this provides more power from the rotor. On the other hand, with the extra airflow coming from the air ducts, the flow separation behind the wing is delayed and the stall effect is shifted to higher wind speeds and the rotor can work for a longer time at nominal power. Besides, since all this is done with passive flow control, a remarkable increase in annual electricity production from the turbine is provided.
Assessment of risk factors of lymph node metastasis and prognosis of Siewert II/III adenocarcinoma of esophagogastric junction: a retrospective study
Authors: Zheng Zhi , Zhang Haiqiao , Xu Rui , Yin Jie , Cai Jun , Zhang Jun , Chen Guangyong , Zhang Zhongtao 
Published: February 2020

Abstract
Background Adenocarcinoma of esophagogastric junction (AEG) has a high incidence, while the extent of lymph node dissection (LND) and prognosis are still controversial. The present study aimed to explore the risk factors of lymph node metastasis (LNM) and prognosis in Siewert II/III AEG patients. Methods A total of 134 patients who underwent surgical operation between July 2013 and December 2019 at the Beijing Friendship Hospital were retrospectively reviewed. The patients were followed up until January 2020. The data were analyzed using logistic regression. Survival analyses were performed using Cox regression. Results Multivariate analysis revealed that the parameters infiltration depth (OR=4.341, 95%CI: 2.498-7.545, P=0.000), gross type (OR=3.626, 95% CI: 1.425-9.228, P=0.007) and intravascular cancer embolus (OR=2.888, 95%CI: 1.106-7.544, P=0.030) correlated with LNM. For all patients, the lymph nodes No. 1, 2, 3, 4, 7, 11 indicated higher lymph node metastatic rate in the abdominal cavity. However, No.5 and No. 6 indicated different tendency, which was higher in Siewert III AEG and lower in Siewert II AEG patients. Mediastinal LNM of Siewert II AEG mainly occurred in No. 110 and No. 111 cases corresponding to 7.1 and 3%, respectively, compared with those noted in Siewert III AEG patients. The 3-year overall survival analysis revealed that LNM ( P= 0.046) and chemotherapy ( P= 0.007) exhibited significant differences. Conclusion The infiltration depth, gross type and intravascular cancer embolus were independent risk factors of LNM. For Siewert II AEG patients, it is reasonable to remove both celiac and mediastinal lymph nodes. This was noted notably for No.1, 2, 3, 4, 7, 11, 110 and No.111. For No.5 and No.6 lymph nodes dissection was not required. For Siewert III AEG patients, mediastinal LND was not required. However, it was necessary to perform pyloric lymphadenectomy, which was performed for No.5 and No.6 lymph nodes. In addition, patients with LNM exhibited worse long-term prognosis. The data indicated that perioperative chemotherapy could improve the prognosis of AEG patients.
The Activation of NLRP3 Inflammasome Potentiates the Immunomodulatory Abilities of Mesenchymal Stem Cells in Murine Colitis Model
Authors: Ahn Ji-Su , Seo Yoojin , Oh Su-Jeong , Yang Ji Won , Shin Ye Young , Lee Byung-Chul , Kang Kyung-Sun , Sung Eui-Suk , Lee Byung-Joo , Shin Tae-Hoon , Kim Hyung-Sik 
Published: February 2020

Abstract
Background Inflammasomes are cytosolic, multiprotein complexes which act at the frontline of the immune responses by recognizing pathogen or danger-associated molecular patterns of pathogens or abnormal host molecules. Mesenchymal stem cells (MSCs) have been reported to possess multipotency to differentiate into various cell types and immunoregulatory effects which make them a promising treatment for regenerative medicine and immune-related diseases, respectively. However, little is known about the expression and role of the inflammasome in adult stem cells. In this study, we investigated the expression and functional regulation of NLRP3 inflammasome in human umbilical cord blood-derived MSCs (hUCB-MSCs). Methods The expression of NLR Family Pyrin Domain Containing 3 (NLRP3) inflammasome was detected in hUCB-MSCs. Cell proliferation, death and differentiation were analyzed after NLRP3 inflammasome activation. To investigate the changes in immunoregulatory functions of hUCB-MSCs, naïve or NLRP3 inflammasome-stimulated cells were infused into chemically induced colitic mice and symptoms were monitored. Results NLRP3 inflammasome activation suppressed the differentiation of hUCB-MSCs into osteoblasts, which was restored when the expression of adaptor proteins for inflammasome assembly was inhibited. Moreover, the suppressive effects of MSCs on T cell responses and the macrophage activation were augmented in response to NLRP3 activation. In vivo studies using colitic mice revealed that the protective abilities of hUCB-MSCs increased after NLRP3 stimulation. Conclusions Our findings suggest that the NLRP3 inflammasome components are expressed in hUCB-MSCs and its activation can regulate the differentiation capability and the immunomodulatory effects of hUCB-MSCs.
Exosomes derived from human umbilical cord MSCs rejuvenate aged MSCs and enhance their functions for myocardial repair
Authors: Zhang Ning , Zhu JinYun , Ma QunChao , Zhao Yun , Wang YingChao , Hu XinYang , Chen JingHai , Zhu Wei , Han ZhongChao , Yu Hong 
Published: February 2020

Abstract
Backhround: Age and other cardiovascular risk factors have been reported to impair the activities of mesenchymal stem cells (MSCs), which will affect the efficacy of stem cell transplantation. The objective of the study is to investigate whether exosomes derived from human umbilical cord MSCs (UMSCs) could enhance the activities of bone marrow MSCs from old person (OMSCs), and improve their capacity for cardiac repair. Methods: Exosomes extracted from conditioned medium of UMSCs were used to treat OMSCs to generate OMSCsExo. The key molecule in the exosomes that have potential to rejuvenate aged MSCs were screened, and the role of OMSC was tested in the mouse model of mycardiac infarction(MI). Results: We found the activity of senescence-associated β-galactosidase and the expression of aging-related factors such as p53, p21, and p16 were significantly higher in OMSCs than those in UMSCs. After treatment with UMSC exosomes, these senescence phenotypes of OMSCs were remarkably reduced. The proliferation, migration, differentiation, anti-apoptotic and paracrine effect were increased in OMSCsExo. In vivo study, mice with cardiac infarction had significantly better cardiac function, less fibrosis, and more angiogenesis after they were injected with OMSCsExo as compared with those with OMSC. There was more miR-136 expression in UMSCs and OMSCsExo than in OMSCs. Upregulation of miR-136 by transfection of miR-136 mimic into OMSCs significantly attenuated the apoptosis and senescence of OMSCs. Apoptotic peptidase activating factor (Apaf1) was found to be the downstream gene that is negatively regulated by miR-136 via directly targeting at its 3’UTR. Conclusion: Our data suggest that exosomes from young MSCs can improve activities of aged MSCs and enhance their function for myocardial repair by transferring exosomal miR-136 and downregulating Apaf1.
Knockdown of lncRNA LINC00958 inhibits the proliferation and migration of NSCLC cells by miR-204-3p/KIF2A axis
Authors: Qi Guan-Bin , Li Lei 
Published: February 2020

Abstract
Background: LINC00958, a newly identified lncRNA, has been reported to be closely linked to tumorigenesis in multiple cancers. However, its specific role in non-small cell lung cancer (NSCLC) remains unclear.Methods: The expression of LINC00958 was determined by RT-qPCR analysis. Cell proliferation and migration were evaluated by CCK-8 and transwell assays, respectively. Xenograft tumor models were established to examine the effect of LINC00958 on tumor growth in vivo. Luciferase reporter assays were performed to determine the interaction between LINC00958 and miR-204-3p and the interaction between miR-204-3p and KIF2A.Results: We found that LINC00958 was up-regulated in NSCLC tissues and cell lines. Down-regulation of LINC00958 inhibited cell proliferation and migration in vitro and suppressed tumor growth in vivo. Mechanically, we revealed that LINC00958 influenced NSCLC progression partly by sponging miR-204-3p and regulating KIF2A expression.Conclusions: Our study provided new insights into the role of LINC00958 as a promising prognostic biomarker and a therapeutic target for NSCLC.
Association between the nurse work environment and severe maternal morbidity in high-income countries: A systematic review protocol
Authors: Brown Kyrah K , Smith Jessica G. , Jeffers RaeAnna L , Pierre Claudy Jean 
Published: February 2020

Abstract
Background The United States has the highest maternal mortality and morbidity rates compared to its high-income peer nations. In high-income nations, a considerable amount of maternal morbidity cases are preventable and linked to provider-related factors. Better nurse work environments are associated with positive patient outcomes, but little is known about its impact on maternal morbidity. In this systematic review, we aim to identify the association between nurse work environment and maternal morbidity, specifically in high-income countries. Methods This systematic review will include original articles on the association between nurse work environment and maternal morbidity. CINAHL, PubMed/Medline and the Cochrane Central Register of Controlled Trials will be searched to retrieve potential original articles that are published between 1990 and 2019 in English language. Citations will be screened by two reviewers, in two rounds, for inclusion based on a priori inclusion and exclusion criteria. Data extraction templates will be populated with data to evaluate the methodological and reporting quality of each study. A combination of structured narrative synthesis and quantitative summaries in tabular format will allow for discussion and recommendations for future research. Discussion Results from this systematic review will provide evidence to elucidate the association between nurse work environment and maternal morbidity. While there is strong evidence demonstration the relation between nurse work environment and general patient outcomes, less is known about its influence on maternal morbidity. Findings from this review will help to guide research in the field and nursing professional in the development of targeted practices and policies aimed at reducing the rates of maternal morbidity.
Pursue today and assess tomorrow - how students’ subjective perceptions influence their preference for self- and peer assessments
Authors: Melser Meskuere Capan , Lettner Stefan , Bäwert Andjela , Puttinger Claudia , Holzinger Anita 
Published: February 2020

Abstract
Background Alternative assessments engage students in the assessment process to improve both short- and long-term outcomes by developing their judgments and responsibility about their own learning, and that of their peers. In this study, we investigated students’ perception towards self- and peer-assessment, their objectivity and impact on students’ learning. Methods The study was conducted at the Medical University of Vienna. Attitudes of second year undergraduate medical students towards self- and peer-assessment, and their objectivity, appropriateness, and the impact of these assessments on students’ learning activities, was inquired using a self-developed questionnaire. Results 423 students participated in this study. Self-assessment was found more appropriate method to assess students’ knowledge. Most of students agreed that peer-assessment is not objective (M= -0.07). Majority of students evaluated that peer assessment has no or little impact on their active and passive learning (M= -0.23, -0.35), on the other hand self-assessment was reported as a helpful tool for gaining long-term knowledge (M= 0.13) and following the content of courses (M= 0.16). Conclusion Based on our results, students’ perspective on peer assessment were negative, on the whole, students had positive attitudes towards self-assessment and negative attitudes towards peer-assessment. This study also determined that self-assessment leads to the promotion of students’ learning.