bluuebunny/crossref_metadata_2025_split huggingface dataset:
When COVID-19 Enters in a Community Setting: An Exploratory Qualitative Study of Community Perspectives on COVID-19 Affecting Mental Well-Being
Authors: Ali Naureen Akber , Feroz Anam Shahil , Ali Noshaba , Feroz Ridah , Meghani Salima Nazim , Saleem Sarah 
Published: August 2020

Abstract
Background: The COVID-19 pandemic has certainly resulted in an increased level of anxiety and fear among the general population related to its management and infection spread. Due to the current unprecedented situation the normal routine life of every individual has been hindered which may cause florid mental distress. Considering the relevance of present circumstances we explored perceptions and attitudes of community members towards COVID-19 pandemic and its impact on their mental well-being. Methods: We conducted an exploratory qualitative study using a purposive sampling approach, at two communities of Karachi, Pakistan. In-depth interviews were conducted with community members including, young adults, middle-age adults, and older adults of both genders. Study data was analyzed manually using the conventional content analysis technique. Results: A total of 27 in-depth interviews were conducted, between May and June, 2020. Three overarching themes were identified: (I) Impact of COVID-19 on mental health of the general communities; (II) Current coping mechanisms to adapt to the new reality; and (III) Recommendations to address mental health of communities. Generally community members underwent increased anxiety and fear due to the contagious nature of the virus. Alongside, social, financial and religious repercussions of the pandemic have also heightened psychological distress among community members. However, community members were able to point out some of the coping mechanisms such as getting closer to God, connecting with family, participating in mental health sessions and resetting lives by indulging in diverse activities. Simultaneously, they also recommended the need of remote mental health services for elders and continuous efforts by the government to address mental health needs of the community at larger scale.Conclusion: COVID-19-associated mental health consequences have hit every individual in the society. The study finding has the potentialto guide the development of context-specific innovative mental health programs to overcome the pandemic repercussions.
Early coronary angiography and percutaneous coronary intervention for out of hospital cardiac arrest survivors without ST-segment elevation: a systematic review and meta-analysis
Authors: Huang Po , Liu Qingquan , Guo Yuhong , Li Bo , Fang Xiaolei 
Published: August 2020

Abstract
Objective: The meta-analysis aims to identify whether out of hospital cardiac arrest (OHCA) survivors of non ST-segment elevation (NSTE) can benefit from early coronary angiography (CAG) and percutaneous coronary intervention (PCI).Methods: The relevant studies from MEDLINE, Cochrane Library, Embase were searched by two independent investigators using a variety of keywords. Stata software (version 12.0, Stata Corp LP, College Station, TX, USA) was used for statistical analysis. Results: A total of 12 studies (9 observational studies, 1 cohort study and 2 randomized control trials) were identified and incorporated into the meta-analysis. For overall analysis, the strategy of early angiography was associated with decreased short-term (hospital discharged) mortality (RR=0.72, 95% CI=0.56-0.93, P=0.000) and long-term (follow up) mortality (RR=0.84, 95% CI=0.71-0.99, P=0.007). However, when analyzed in the subgroup of randomized controlled study, the strategy of early angiography didn’t have survival benefit in the randomized controlled study group for short-term mortality (RR=1.12, 95% CI=0.89-1.41, P=0.331) and long-term mortality (RR=1.06, 95% CI=0.85-1.32, P=0.572). Meanwhile, our analysis found that, if early CAG performed, PCI followed by CAG is not associated with hospital discharged mortality (RR=1.14, 95% CI=0.96-1.37, P=0.132) compared with CAG alone. No significant differences between the groups were found in the remaining secondary endpoints.Conclusion: Due to the observational nature of the studies available, we may consider that early CAG and PCI is not be recommended for patients with NSTE OHCA.
CDK5/CAMK2-DRP1-AMPK-Mediated Mitochondrial Fission is Required for Imatinib Resistance of CML Cells
Authors: Lu Ziyuan , Tang Jiaming , Li Yuling , Liang Guohua , Chen Xiaoyun , Luo Bing , Qu Qian 
Published: August 2020

Abstract
Background: In chronic myeloid leukemia (CML), resistance to tyrosine kinase inhibitors (TKIs) is still a serious clinical challenge, especially in the context of multi-resistance BCR-ABL mutations, such as T315I. Increasing evidence has demonstrated that mitochondrial fission plays an important role in cancer stem cell maintenance and drug resistance. Therefore, as a vital fission-related protein, DRP1 serves as a novel target for cancer treatment. However, whether DRP1 inhibition could overcome imatinib resistance in CML remains unknown.Methods: We used bioinformatic analysis and a confocal microscopy experimental approach to investigate whether mitochondrial fission could be involved in drug resistance in CML. Next, we applied biological and metabolic detection methods to reveal the roles of DPR1 in autophagy, apoptosis, and mitochondrial respiration in CML cells and determine whether coupling imatinib with DRP1 inhibition has a synergetic anti-leukemic effect on both drug-sensitive and drug-resistant CML cells. Results: Resistant CML cells displayed fragmented mitochondria and were accompanied by overexpression of active DRP1 compared with their sensitive counterparts. Furthermore, blocking DRP1 decreased mitochondrial respiration via the CDK5/CAMK2-DRP1-AMPK signaling pathway. Additionally, DRP1 inhibition perturbed the balance between mitochondrial-dependent apoptosis and autophagy, which led to cell death. Finally, combination treatment with imatinib and DRP1 inhibition exhibited a synergetic anti-leukemic effect on both drug-sensitive and drug-resistant CML cells (including CML cells carrying T315I mutation) in in vitro or in vivo experiments.Conclusions: Combination treatment with imatinib and DRP1 inhibition may represent a promising strategy to overcome CML drug resistance.
OrtSuite – a flexible pipeline for annotation of ecosystem processes and prediction of putative microbial interactions
Authors: Saraiva João Pedro , Gomes Marta , Kallies René , Vogt Carsten , Chatzinotas Antonis , Stadler Peter , Dias Oscar , Rocha Ulisses 
Published: August 2020

Abstract
Background: The exponential increase in high-throughput sequencing data and the development of computational sciences and bioinformatics pipelines has advanced our understanding of microbial community composition and distribution in complex ecosystems. Despite these advances, the identification of microbial interactions from genomic data remains a major bottleneck. To address this challenge, we present OrtSuite, a flexible workflow to predict putative microbial interactions based on genomic content. Results: OrtSuite combines ortholog clustering strategies with genome annotation based on a user-defined set of functions allowing for hypothesis-driven data analysis. OrtSuit allows users to install and run all workflow components and analyze the generated outputs using a simple pipeline consisting of 23 bash commands and one R command. Annotation is based on a two-stage process. First, only a subset of sequences from each ortholog cluster are aligned to all sequences in the Ortholog-Reaction Association database (ORAdb). Next, all sequences from clusters that meet a user-defined identity threshold are aligned to all sequence sets in ORAdb to which they had a hit. This approach results in a decrease in time needed for functional annotation. Further, OrtSuit identifies putative interspecies interactions based on their individual genomic content based on constrains given by the users. Additional control is afforded to the user at several stages of the workflow: 1) The construction of ORAdb only needs to be performed once for each specific process also allowing manual curation; 2) The identity and sequence similarity thresholds used during the annotation stage can be adjusted; and 3) Constraints related to pathway reaction composition and known species contributions to ecosystem processes can be defined. Conclusions: OrtSuit is an easy to use workflow that allows for rapid functional annotation based on a user curated database. Further, this novel workflow allows the identification of interspecies interactions through user-defined constrains. Due to its low computational demands, for small datasets (e.g. maximum 100 genomes) OrtSuit can run on a personal computer. For larger datasets (> 100 genomes), we suggest the use of computer clusters. OrtSuit is an open-source software available at https://github.com/mdsufz/OrtSuit .
Study on the COVID-19 infection status, prevention and control strategies among entry people in Shenzhen
Authors: Wang Jing-Zhong , Zou Xuan , Xu Zi-Qian , Wang Hai-Rui , Wang Bi-Xin , He Jian-Fan 
Published: August 2020

Abstract
Background The COVID-19 confirmed cases overseas continue to rise for months, while people overseas prefer to return at present. It is risky to have a large number of infected imported cases which may cause COVID-19 spread to China and even lead to outbreak again. In order to prevent imported infection, Shenzhen implemented the losed-loop management strategy by taking nucleic acid testing (NAT) for severe acute respiratory syndromes coronavirus 2 (SARS-CoV-2) and medical observation for 14 days among individuals who have epidemic history (Hong Kong, Macao, Taiwan province and other countries) within 14 days. Our study described the status of COVID-19 infection among entry people in Shenzhen, and also evaluated the effect of closed-loop management strategy.Methods A total of 86,844 people overseas entered Shenzhen from January 1 to April 18, 2020, and there were 39 imported cases. We made a descriptive study by analyzing the entry time, reported time, local confirmed cases in origin countries, and the number of entry people from abroad. The NAT were completed in Shenzhen center for disease control and prevention (CDC), ten district-level CDCs, as well as fever clinics.Results The infection rate of entry people was 4.49‰ (95% CI: 3.26‰ − 6.05‰). Most of the entry people or imported cases have Chinese nationality. The number of entry people and imported cases in Nanshan and Futian districts were larger than others. 15.73% of the entry people came from the US, and 12.67% came from the UK. 14 imported cases (35.9%) came from the UK, 9 (23.08%) came from the US. The imported risks from the US and UK in Shenzhen were higher than other countries or regions. According to the 14-days’ incubation period and the number of entry people, individuals from the US since Mar 9 were the high-risk population. Accordingly, entry people from the UK since Mar 13 were the high-risk population. It is important to evaluate the imported risk by analyzing local confirmed cases status in origin countries or regions and the number of entry people from these countries or regions to Shenzhen. The distribution of entry time and report time for imported cases in Shenzhen were similar. So it is important to prevent and control COVID-19 imported infection by taking NAT and medical observation at port.Conclusions It is effective to implement closed-loop management strategy for individuals who have epidemic history (Hong Kong, Macao, Taiwan province and other countries) within 14 days. In order to control COVID-19 outbreak, we need the collaboration and cooperation at the global, national, and subnational levels to prevent, detect, and respond effectively.
Determinants of Exclusive Breast Feeding Practice among HIV Positive Mothers in North East Ethiopia: A mixed method study
Authors: Girma Yonas Bizuwork , Minda Abebe , Hailu Awraris , Zegeye Betregiorgis Hailu 
Published: August 2020

Abstract
Background: Exclusive breast feeding (EBF) of Human Immune Virus (HIV) exposed infants for the first six months is strongly recommended and vital for protecting them against common childhood illnesses including diarrhea and pneumonia. Despite its benefit, EBF practice is low in developing countries including Ethiopia. There is a paucity of evidence for factors associated with EBF among HIV positive mothers. Objectives: To assess exclusive breast feeding practice and its associated factors among HIV positive mothers attending Prevention of mother to child transmission and Anti-retroviral therapy clinics in public Health facilities of Debre Birhan town Amhara National Regional State, Ethiopia from February 01, 2020 to Apr 30, 2020Methods: Facility based convergent mixed methods design was used to conduct the study in public Health facilities of Debre Birhan town. Structured interviewer-administered questionnaires were used to collect data from 432 participants selected by systematic random sampling technique. Epi info version 7 and SPSS version 20 were used for data entry and analysis respectively. Descriptive and inferential statistics were computed during the analysis. Two focus group discussions and 25 in-depth interviews and observational checklist were held to collect data on barriers for exclusive breast feeding practice and thematic analysis was used to identify important insights on the study topic. Bivariate and multivariate logistic regression were conducted to select candidate variable and determine adjusted effect of independent variables respectively using p-value less than 0.05. The output from multivariate logistic regression were reported with 95% confidence interval.Results: The prevalence of exclusive breast feeding, mixed feeding and exclusive formula feeding practice were 89.8%, 6.9% and 3.2% respectively. Having information about EBF (No=0.02, 95% CI; 0.01, 0.12), time taking from home to workplace (less than 30 min=4.96, 95% CI; 1.17, 20.95), disclosing HIV status (No= 0.09, 95% CI; 0.02, 0.37), place of residence (urban=5.37, 95% CI; 1.12, 25.77), living with mother/mother in-law (No=6.03, 95% CI; 1.26, 28.86), knowledge about EBF (poor knowledge=0.06, 95% CI; 0.01, 0.34) were main factors for exclusive breast feeding practice. Poor counseling, non-disclosure of HIV status and pressure from mother/mother in-law were the commonly raised reasons by FGD and IDI participants for non-exclusive breast feeding practice. Conclusions: Majority of HIV positive mothers were practicing exclusive breast feeding for the first six month. Strengthening information dissemination, providing quality health services and empowering mothers through integrated interventions help to promote exclusive breast feeding practice among mothers living with HIV.
Combinatorial therapeutic plan for COVID-19 treatment armed up with antiviral, antiparasitic, cell-entry inhibitor, and immune-boosters
Authors: Chattopadhyay Nabanita Roy , Chatterjee Koustav , Banerjee Antara , Choudhuri Tathagata 
Published: August 2020

Abstract
SARS-CoV-2, or novel coronavirus, is causing the fatal and contagious coronavirus disease-2019 (COVID-19) affecting thousands of people every single day. Researchers are continuously searching for any possible cure and/or vaccine, but no conclusive report is available till date. Like many others, we realize that a rapid, immediate, and elaborate strategy must be adopted to protect mankind. To avoid the time-loss due to clinical trials, we have tested some FDA-approved drugs to combat COVID-19. We accessed information from public databases and publications, and studied the mechanism of infection of SARS-CoV-2 and the interactions of various drugs with SARS-CoV-2 proteins in silico. We found a few antivirals and antiparasitic drugs to interact with important SARS-CoV-2 proteins. Particularly Galidesivir, Remdesivir, and Pirodavir are the chosen antiviral drugs; and Proguanil, Mefloquine, and Artesunate are the chosen antiparasitic drugs. In addition, inhibitors to prevent host-cell entry and a few supportive immuneboosters can be used in different combinations. Our study proposes a four-way attack to this fatal virus for the possible management of COVID-19 armed up with an antiviral, an antiparasitic drug, a cell-entry inhibitor, and a few supportive immune-boosters, which can be used in different combinations in different groups of people.
MAP3K1 Expression is Associated With Progression and Poor Prognosis of Hormone Receptor-Positive, HER2-Negative Early-Stage Breast Cancer
Authors: Kuo Sung-Hsin , Wei Ming-Feng , Lee Yi-Hsuan , Yang Wen-Chi , Yang Shi-Yi , Lin Jui-Chueh , Huang Chiun-Sheng 
Published: August 2020

Abstract
Background: MAP3K1 (Mitogen-Activated Protein Kinase Kinase Kinase 1) participates in the MAPK signal transduction pathway, responding to a number of mitogenic and metabolic stimuli, which may be related to breast cancer susceptibility and progression. In this study, we assessed whether overexpression of MAP3K1 promotes proliferation, migration, and invasion of breast cancer cells, and thus, affects the prognosis of hormone receptor (HR)-positive, human epidermal growth factor receptor 2 (HER2)-negative early-stage breast cancer. Methods: Two HR-positive, HER2-negative breast cancer cell lines (MCF7 and T-47D), both overexpressing MAP3K1, were transfected with MAP3K1 short hairpin RNA plasmids (shMAP3K1) and their proliferation, migration, and invasion were examined. We assessed whether shMAP3K1 affects the cell cycle and levels of downstream signaling molecules, such as ERK and NF-κB, as well as sensitizes cells in both cell lines to chemotherapeutic and hormonal agents. A total of 161 patients with HR-positive, HER2-negative T1-T2 breast cancer and 0 to 3 nodal metastases were included. The expression of MAP3K1 and phospho (p)-ERK proteins was assessed by immunohistochemistry. Results: In both cell lines (MCF7 and T-47D), shMAP3K1 significantly reduced cell growth, migration, and invasion by downregulating MMP-9 and blocking the G2/M phase of the cell cycle and its regulatory molecule cyclin B1. Besides, shMAP3K1 downregulated ERK- and NF-κB-dependent gene transcription, and enhanced sensitivities of both cell lines to doxorubicin, docetaxel, and tamoxifen. Patients with MAP3K1 overexpression exhibited significantly poor 9-year disease-free survival (DFS; 72.6% vs. 88.5%, p = 0.022) and overall survival (OS; 83.8% vs. 96.2%, p = 0.012) relative to those without MAP3K1 overexpression. Furthermore, the p-ERK expression was significantly associated with MAP3K1 expression (p < 0.001) and correlated with a poor 9-year DFS (p = 0.033) and OS (p = 0.023). Conclusions: Our results indicate that overexpression of MAP3K1 plays a major role in poor prognosis of HR-positive, HER2-negative early-stage breast cancer.
Work-Related and Personal Factors Associated With Mental Well-Being During the COVID-19 Response: Survey of Health Care and Other Workers
Authors: Evanoff Bradley A , Strickland Jaime R , Dale Ann Marie , Hayibor Lisa , Page Emily , Duncan Jennifer G , Kannampallil Thomas , Gray Diana L 
Published: August 2020

Background
The response to the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has created an unprecedented disruption in work conditions. This study describes the mental health and well-being of workers both with and without clinical exposure to patients with coronavirus disease (COVID-19).

Objective
The aim of this study is to measure the prevalence of stress, anxiety, depression, work exhaustion, burnout, and decreased well-being among faculty and staff at a university and academic medical center during the SARS-CoV-2 pandemic and describe work-related and personal factors associated with their mental health and well-being.

Methods
All faculty, staff, and postdoctoral fellows of a university, including its medical school, were invited in April 2020 to complete an online questionnaire measuring stress, anxiety, depression, work exhaustion, burnout, and decreased well-being. We examined associations between these outcomes and factors including work in high-risk clinical settings and family/home stressors.

Results
There were 5550 respondents (overall response rate of 34.3%). Overall, 34% of faculty and 14% of staff (n=915) were providing clinical care, while 61% of faculty and 77% of staff were working from home. Among all workers, anxiety (prevalence ratio 1.37, 95% CI 1.09-1.73), depression (prevalence ratio 1.28, 95% CI 1.03-1.59), and high work exhaustion (prevalence ratio 1.24, 95% CI 1.13-1.36) were independently associated with community or clinical exposure to COVID-19. Poor family-supportive behaviors by supervisors were also associated with these outcomes (prevalence ratio 1.40, 95% CI 1.21-1.62; prevalence ratio 1.69, 95% CI 1.48-1.92; and prevalence ratio 1.54, 95% CI 1.44-1.64, respectively). Age <40 years and a greater number of family/home stressors were also associated with these poorer outcomes. Among the subset of clinicians, caring for patients with COVID-19 and working in high-risk clinical settings were additional risk factors.

Conclusions
Our findings suggest that the pandemic has had negative effects on the mental health and well-being of both clinical and nonclinical employees. Mitigating exposure to COVID-19 and increasing supervisor support are modifiable risk factors that may protect mental health and well-being for all workers.
Em briga de marido e mulher, o Poder Público mete a colher: mídia, mobilizações coletivas e estratégias de visibilização da violência doméstica em Portugal e no Brasil
Authors: Silva Edilson Márcio Almeida da 
Published: August 2020

Dadas as transformações relativas a produção, armazenamento e fluxo de informações no mundo contemporâneo, a visibilidade dos fenômenos sociais encontra-se, não raro, relacionada ao modo como estes são tratados pela mídia. Por consequência, considerada a especificidade de sua participação na definição do que é socialmente relevante, a mídia contribui de forma decisiva para o agendamento das políticas públicas nas mais diversas áreas, inclusive na de segurança. A fim de discutir a capacidade de “agendamento” dos veículos de comunicação sobre os formuladores de políticas públicas, tomar-se-á como exemplo o processo de criminalização da violência doméstica em Portugal, procurando assinalar a importância estratégica da mídia na sua visibilização e, por corolário, na sua construção como um dos principais problemas públicos do país. O objetivo geral do trabalho é, ainda que de forma sucinta, estabelecer uma perspectiva comparativa e contrastiva com o que, a esse respeito, se verifica no Brasil.