bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nkx2-5 defines distinct scaffold and recruitment phases during formation of the cardiac Purkinje fiber network
Authors: Choquet Caroline , Kelly Robert G. , Miquerol Lucile 
Published: March 2020

AbstractThe ventricular conduction system coordinates heartbeats by rapid propagation of electrical activity through the Purkinje fiber (PF) network. PFs share common progenitors with contractile cardiomyocytes, yet the mechanisms of segregation and network morphogenesis are poorly understood. In this study, we apply genetic fate mapping and temporal clonal analysis to identify cardiomyocytes committed to the PF lineage as early as E7.5. We find that a polyclonal PF network emerges by progressive recruitment of conductive precursors to this scaffold from a pool of bipotent progenitors. At late fetal stages, the segregation of conductive cells increases during a phase of rapid recruitment to build the definitive PF network through a non-cell autonomous mechanism. We also show that PF differentiation is impaired in Nkx2-5 haploinsufficient embryos leading to failure to extend the scaffold. In particular, late fetal recruitment fails, resulting in PF hypoplasia and persistence of bipotent progenitors. Our results identify how transcription factor dosage regulates cell fate divergence during distinct phases of PF network morphogenesis.
“The Heidelberg Five” Personality Dimensions: Genome-wide Associations, Polygenic Risk for Neuroticism, and Psychopathology 20 Years after Assessment
Authors: Heilbronner Urs , Papiol Sergi , Budde Monika , Andlauer Till F. M. , Strohmaier Jana , Streit Fabian , Frank Josef , Degenhardt Franziska , Heilmann-Heimbach Stefanie , Witt Stephanie , Forstner Andreas J. , Loerbroks Adrian , Amelang Manfred , Stürmer Til , Müller-Myhsok Bertram , Nöthen Markus M. , Rietschel Marcella , Schulze Thomas G. 
Published: March 2020

ABSTRACTThe HeiDE study (‘‘Heidelberger Langzeitstudie zu Risikofaktoren und Diagnose chronischer Erkrankungen’’) is a longitudinal population-based study that started in the 1990s and, at baseline, assessed an array of health-related personality questionnaires in 5 133 individuals. Five latent personality dimensions (The Heidelberg Five) were identified and interpreted as Emotional Lability (ELAB), Lack of Behavioral Control (LBCN), Type A Behavior (TYAB), Locus of Control over Disease (LOCC), and Psychoticism (PSYC). A subset of participants (n=3 268; after quality control) were genotyped on whole-genome arrays at follow-up. To further characterize The Heidelberg Five, we analyzed genomic underpinnings, their relations to the genetic basis of the Big Five trait Neuroticism, and longitudinal associations with lifetime psychiatric symptoms. SNP-based heritability was significant for ELAB (34%) and LBCN (29%). Five separate genome-wide association studies (GWAS) using factor scores on personality dimensions as phenotypes were conducted, only the phenotype PSYC yielded a genome-wide significant finding (p<5×10−8, top SNP rs138223660). Gene-based analyses identified significant findings for ELAB (Integrin Subunit Beta 5), TYAB (Coiled-coil Domain Containing 83), and PSYC (Nuclear Receptor Subfamily 1 Group H Member 4). Polygenic risk scores for Neuroticism, phenotypically related to ELAB, were associated with ELAB, but not with the remaining Heidelberg Five. Longitudinally, all personality dimensions were related to depressive symptoms at follow-up, with ELAB, LBCN, and PSYC also associated with lifetime anxiety symptoms. These results highlight the clinical importance of health-related personality traits, and identify LBCN as a heritable “executive function” personality trait.
A generative algorithm for de novo design of proteins with diverse pocket structures
Authors: Basanta Benjamin , Bick Matthew J , Bera Asim K , Norn Christoffer , Chow Cameron M , Carter Lauren P , Goreshnick Inna , Dimaio Frank , Baker David 
Published: March 2020

AbstractTo create new enzymes and biosensors from scratch, precise control over the structure of small molecule binding sites is of paramount importance, but systematically designing arbitrary protein pocket shapes and sizes remains an outstanding challenge. Using the NTF2-like structural superfamily as a model system, we developed a generative algorithm for creating a virtually unlimited number of de novo proteins supporting diverse pocket structures. The generative algorithm was tested and refined through feedback from two rounds of large scale experimental testing, involving in total, the assembly of synthetic genes encoding 7896 generated designs and assessment of their stability on the yeast cell surface, detailed biophysical characterization of 64 designs, and crystal structures of 5 designs. The refined algorithm generates proteins that remain folded at high temperatures and exhibit more pocket diversity than naturally occurring NTF2-like proteins. We expect this approach to transform the design of small molecule sensors and enzymes by enabling the creation of binding and active site geometries much more optimal for specific design challenges than is accessible by repurposing the limited number of naturally occurring NTF2-like proteins.
Genetic Interplay Between Transcription Factor Pou4f1/Brn3a and Neurotrophin Receptor Ret In Retinal Ganglion Cell Type Specification
Authors: Muzyka Vladimir Vladimirovich , Badea Tudor Constantin 
Published: March 2020

AbstractWhile the transcriptional code governing retinal ganglion cell (RGC) type specification begins to be understood, its interplay with neurotrophic signaling is largely unexplored. Using sparse random recombination, we show that mosaic gene dosage manipulation of the transcription factor Brn3a/Pou4f1 in neurotrophic receptor Ret heterozygote RGCs results in altered cell fate decisions and/or morphological dendritic defects. Specific RGC types are lost if Brn3a is ablated during embryogenesis and only mildly affected by postnatal Brn3a ablation. Sparse but not complete Brn3a heterozygosity combined with complete Ret heterozygosity has striking effects on RGC type distribution. Brn3a only mildly modulates Ret transcription, while Ret knockouts exhibit normal Brn3a and Brn3b expression. However, Brn3a loss of function significantly affects distribution of Ret co-receptors GFRα1-3, and neurotrophin receptors TrkA and TrkC in RGCs. Based on these observations, we propose that Brn3a and Ret converge onto developmental pathways that control RGC type specification, potentially through a competitive mechanism requiring signaling from the surrounding tissue.
Epigenetic regulation of Neuregulin-1 tunes white adipose stem cell differentiation
Authors: Cordero Alyssa D. , Callihan Evan C. , Said Rana , Alowais Yasir , Paffhausen Emily S. , Bracht John R. 
Published: March 2020

AbstractExpansion of subcutaneous adipose tissue by differentiation of new adipocytes has been linked to improvements in metabolic health. However, an expandability limit has been observed wherein new adipocytes cannot be produced, the existing adipocytes become enlarged (hypertrophic) and lipids spill over into ectopic sites. Inappropriate ectopic storage of these surplus lipids in liver, muscle, and visceral depots has been linked with metabolic dysfunction. Here we show that Neuregulin-1 (NRG1) serves as a regulator of adipogenic differentiation in subcutaneous primary human stem cells. We further demonstrate that DNA methylation modulates NRG1 expression in these cells, and a 3-day exposure of stem cells to a recombinant NRG1 peptide fragment is sufficient to reprogram adipogenic cellular differentiation to higher levels. These results define a novel molecular adipogenic rheostat with potential implications for the expansion of adipose tissuein vivo.
OCB-E among Chinese employees of different contract types
Authors: Ren Shuang , Tang Guiyao , Kim Andrea 
Published: March 2020

PurposeDrawing on a motivational model of proactive behavior, this study theorizes that employment status, reflective moral attentiveness (RMA), and organization-based self-esteem (OBSE) constitute the can-do, reason-to, and energized-to motivational states, which interact to induce organizational citizenship behavior toward the environment (OCB-E).Design/methodology/approachThe authors conducted random coefficient modeling (RCM) analysis with a multisource, time-lagged data set collected from 235 employees in Chinese firms.FindingsThis RCM analysis found that more OCB-E resulted from standard employees with higher levels of RMA and OBSE.Originality/valueThe value of this research lies in understanding of the antecedents of green behavior at the individual level by identifying specific motivational states and highlighting the coexistence of motivational states in predicting OCB-E. These findings provide new insight into the theory of developing and managing green OCB performers in today's workplace characterized by workforce mixing.
Emotional and social competence in the aestheticization of labor in the restaurant industry
Authors: Genc Volkan , Akoglan Kozak Meryem 
Published: March 2020

Purpose
The purpose of this paper is to provide guidance regarding the satisfaction of customer needs in the competitive restaurant industry. Restaurants have seen a transformation in employees’ labor, changing from primarily physical and mental to emotional and aesthetic dimensions. In this study, the effect of managers’ emotional and social competence (ESC) on the aesthetic labor of service and kitchen employees has been investigated.

Design/methodology/approach
Empirical data was collected from employees and managers of a restaurant chain. Structural equation modeling was the main analytical tool used to assess the results.

Findings
The findings indicated that managers’ ESC affected the aesthetic labor of their service (aesthetic traits (AT), aesthetic requirements and service encounters) and kitchen (AT, aesthetic creativity and aesthetic harmony ) employees. Achievement orientation and adaptability were among the dimensions of emotional competence that contributed the most to aesthetic labor. The most significant elements of social competence were inspiring leadership and conflict management.

Practical implications
The study suggests that managers can improve the aesthetic performance of their employees by using their ESC.

Originality/value
This is the first study of this kind to include kitchen employees while considering the effects of restaurant managers’ ESC on aesthetic labor. The findings indicate the importance of the ESC of managers in improving the aesthetic labor of employees.
Criticality assessment of the barriers to Lean Construction
Authors: Mano Aline Patricia , Gouvea da Costa Sergio E. , Pinheiro de Lima Edson 
Published: March 2020

PurposeThe purpose of this study seeks to identify the critical barriers for the deployment of Lean Construction (LC).Design/methodology/approachIn order to identify the barriers of Lean Construction, a systematic review of the scientific literature was carried out, in addition to analysis of the content of papers presented at a major Lean Construction conference. After the barriers were identified, a Lawshe study was conducted with experts in Lean Construction to verify the criticality of each of the barriers encountered.FindingsThis study identified 83 potential pre-deployment barriers in the literature, of which eight were considered critical by the experts. The results of Lawshe study pointed that critical barriers refer to leadership characteristics, cultural aspects and structural aspects.Research limitations/implicationsVariations in the assessment of the criticality of the barriers may occur from one country to another due to regional differences in the construction sector.Practical implicationsThe implications of this study are recognizing and identifying the characteristics of an organization that can hinder Lean Construction allows these issues to be resolved before beginning the Lean journey.Originality/valueThis article is the first of its kind to assess the criticality of barriers to Lean Construction using a quantitative technique. Knowledge of this set of barriers provides both practical and theoretical readers with an increased chance of success in the deployment of an LC project.
Competitive environment and bank development
Authors: Seguí Alcaraz Antoni 
Published: March 2020

Purpose
Right up to the moment, the financial sector has been considered a clean and a neutral sector, as far as social and environmental impact concerns. The purpose of this paper is to determine the attitude of Spanish credit institutions to the sustainable development.

Design/methodology/approach
Delphi methodology was used to test the viability and validity of the questionnaire and to choose the best systematic way of communication. The research to understand society and environment focussed on investments was conducted using a survey completed by 57 Spanish credit institutions. Various types of statistical analyses were performed to assess the questionnaire.

Findings
The higher the entities’ investment is in environmental projects, the more sensitive they will be about social responsibilities issues. However, the higher the percentage invested in funding to communities or dejected regions and in cultural projects.

Research limitations/implications
To know, measure and evaluate the activities of a credit institution in the environment, it is necessary to define indicators for the policies, procedures and management practices adequate for the sector.

Practical implications
Credit Spanish Institutions sector organizations are responding positively to sustainable development and, in the process, enhancing stakeholder value.

Social implications
In various practices of credit institutions, implementing a preventive environmental approach or undertaking initiatives to promote greater environmental responsibilities – along with the possibility to allocate funds for certain projects – environmental conditions are also considered.

Originality/value
To develop good environmental practices in credit institutions, they apply the principles of sustainability and identify and quantify the environmental risk in the normal internal process of risk assessment.
Anti-money laundering law and policy as a double edged sword
Authors: Mugarura Norman 
Published: March 2020

Purpose
Regulators have a duty to enforce anti-money laundering (AML) and countering financing of terrorism regulation. However, in doing so, they should not to be overzealous especially in carrying out investigations into suspicious money laundering transactions. This does not mean that oversight agencies should not carry out the required investigations with due diligence. This study aims to propose that banks cannot be allowed to operate in a lawless environment; however, there is a need ensure that businesses are able to operate with minimal regulatory interference.

Design/methodology/approach
Data was collected from primary and secondary sources such as Uganda’s Anti-Money laundering Act 2013 (amended 2017), Patriot Act 2001, Proceeds of Crime Act 2000 International legal instruments, case law, books, websites, journal papers, policy documents and scholarly debates and evaluated to foster the objectives of the paper accordingly. The paper has also been enriched by empirical experiences of countries in Europe, Africa and within countries on money-laundering regulation and its intricacies. There was a wealth of online data sources and in print, which were reviewed and internalised to foster the objectives for writing the book.

Findings
Regulation of businesses against money laundering and financing of terrorism imposes a heavy cost burden on poorer countries and should be funded by developed economies for some countries to easily operate desired International AML standards. It also needs to be noted that banks cannot be allowed to operate in a lawless business environment, which makes money laundering an international and national security issue.

Originality/value
The thesis of this paper was drawn from the author’s presentation to security agencies in Kampala in August 2019. In his presentation, the author opined that investigations into money-laundering offences should be triggered when a financial institution forms suspicions of potential money-laundering offences to have been committed. Some of the questions he sought to answer during the presentation was whether sharing information on “accountable persons or the regulated sector” in Uganda’s AML 2013 with newspapers before investigations are concluded does not amount to tipping off presumed money-laundering culprits? How should investigations be conducted?
Bricks, mortar, and proptech
Authors: Saiz Albert 
Published: March 2020

PurposeDigital and information technologies (IT) are becoming silently pervasive in old-fashioned real estate markets. This paper focuses on three important avenues for the diffusion of IT in commercial real estate: online brokerage and sales, the commoditization of space and Fintech in mortgage and equity funding. We describe the main new markets and products created by this IT revolution. The focus is on the pioneering US market, with some attention devoted to the specific firms and institutions taking these innovations into the mainstream. We also carefully analyze the economic underpinnings from which the new technologies can expect to generate cash flows, thus becoming viable—or not. Finally, we discuss their likely impact on established players in the commercial real estate arena.Design/methodology/approachIn this paper, the author chooses to focus on three separate arenas where the IT revolution—sometimes referred to as Proptech, as applied to real estate—is having discernible impacts: sales and brokerage, space commoditization and online finance platforms. The author invites the reader to think seriously about the economic fundamentals that may—or may not—sustain new business models in Proptech. Real estate economists and investors alike need to be critical of new business models, especially when they are being aggressively marketed by their promoters. Trying to avoid any hype, the author provides thoughts about the likely impact of the innovations on their markets, guided by economic and finance theory, and previous experience.FindingsThe author evaluates the evolution of commercial real estate brokerage. While innovations will, no doubt, have an impact on the ways in which we buy and lease commercial properties, the lessons from the housing market should make us skeptical about the possibility of the new technologies dramatically facilitating disintermediation in this market. In fact, new oligopolies seem to be emerging with regard to market data provision.Practical implicationsProptech will change some aspects of the real estate industry, but not others!Originality/valueAs change pervades the property industry, only a relatively few research pieces are illustrating or—more importantly—providing insights about the likely economic and financial impacts of IT penetration. Similarly, only a few papers have so far addressed the economic viability of the alternative business models of tech startups targeting real estate markets and transactions.
Functional replacement of isoprenoid pathways in Rhodobacter sphaeroides
Authors: Orsi Enrico , Beekwilder Jules , van Gelder Dewi , van Houwelingen Adèle , Eggink Gerrit , Kengen Servé W.M. , Weusthuis Ruud A. 
Published: March 2020

SummaryAdvances in synthetic biology and metabolic engineering have proven the potential of introducing metabolic by‐passes within cell factories. These pathways can provide a more efficient alternative to endogenous counterparts due to their insensitivity to host's regulatory mechanisms. In this work, we replaced the endogenous essential 2‐C‐methyl‐D‐erythritol 4‐phosphate (MEP) pathway for isoprenoid biosynthesis in the industrially relevant bacterium Rhodobacter sphaeroides by an orthogonal metabolic route. The native 2‐C‐methyl‐D‐erythritol 4‐phosphate (MEP) pathway was successfully replaced by a heterologous mevalonate (MVA) pathway from a related bacterium. The functional replacement was confirmed by analysis of the reporter molecule amorpha‐4,11‐diene after cultivation with [4‐13C]glucose. The engineered R. sphaeroides strain relying exclusively on the MVA pathway was completely functional in conditions for sesquiterpene production and, upon increased expression of the MVA enzymes, it reached even higher sesquiterpene yields than the control strain coexpressing both MEP and MVA modules. This work represents an example where substitution of an essential biochemical pathway by an alternative, heterologous pathway leads to enhanced biosynthetic performance.
Antenatal vaccine uptake: A cross‐sectional study investigating factors influencing women’s choices in pregnancy
Authors: Moir Drew , Gunter Konrad , Lynch Lee‐Anne , Vogrin Sara , Said Joanne 
Published: March 2020

BackgroundSeasonal influenza and pertussis cause significant morbidity and mortality among expectant mothers and infants. Vaccination during the antenatal period is an important public health intervention, minimising rates of maternal, neonatal and infant infection.AimsThe primary aim of this project was to establish the rates of antenatal vaccine uptake. Secondly, the study aimed to determine socio‐demographic factors significant to vaccine uptake. Thirdly, the project aimed to produce a thematic analysis of the factors affecting vaccination uptake during pregnancy.Materials and MethodsA cross‐sectional observational study was conducted among women attending a large maternity hospital, in the western suburbs of Melbourne, for perinatal care. Data were collected via self‐completed questionnaires after delivery. Data from the questionnaires were entered into an electronic database, and STATA was used to undertake correlation analysis.ResultsOver a 12‐month period 1678 women completed questionnaires and 1305 were eligible for further analysis. The uptake of influenza vaccine was 48.3%, pertussis vaccine uptake was higher, at 82.9%. Uptake of influenza and pertussis vaccines strongly correlated with recommendations from healthcare providers (odds ratios 29.7 and 63.8 respectively). Maternal country of birth, age and parity were significant predictors of vaccine uptake. In thematic analysis, healthcare provider recommendation and the perceived risk of the disease were factors resulting in vaccination.ConclusionThis study determined the rate of antenatal vaccine uptake and significant socio‐demographic determinants affecting uptake at a large maternity hospital in metropolitan Melbourne. Ensuring healthcare providers recommend vaccination is likely to improve coverage.