bluuebunny/crossref_metadata_2025_split huggingface dataset:
Efficacy of ultrasound-guided erector spinae plane block on postoperative quality of recovery and analgesia after modified radical mastectomy: randomized controlled trial
Authors: Yao Yusheng , Li Hao , He Qiaolan , Chen Tingting , Wang Yihang , Zheng Xiaochun 
Published: November 2019

BackgroundErector spinae plane block (ESPB) is a novel regional anesthesia technique that is gaining popularity for postoperative pain management. This randomized controlled trial evaluated the effect of ESPB on quality of recovery (QoR) in patients undergoing modified radical mastectomy.MethodsEighty-two female patients undergoing modified radical mastectomy were included. Patients were randomly assigned to receive preoperative ultrasound-guided ESPB with either 0.5% ropivacaine or saline. The primary outcome was QoR, assessed 24 hours postoperatively using the 15-item QoR questionnaire (QoR-15). Secondary outcomes included postoperative pain scores, postoperative cumulative opioid consumption, postanesthesia care unit (PACU) discharge time, postoperative nausea or vomiting and dizziness.ResultsGlobal QoR-15 scores 24 hours postoperatively were significantly higher (indicating better quality) in the ESPB group (median 120, IQR 118–124) compared with the control group (median 110, IQR 108.3–112.8), with a median difference of 10 (95% CI 9 to 12, p<0.001). Compared with the control group, ESPB with ropivacaine reduced pain scores up to 8 hours after surgery, as well as reduced postoperative cumulative opioid consumption and PACU discharge time.ConclusionsA single preoperative injection of ESPB with ropivacaine may improve QoR postoperatively and acute postoperative analgesia in patients undergoing a modified radical mastectomy.Trial registration numberChiCTR-1800019599.
Extracellular DNA Containing (dG)n Motifs Penetrates into MCF7 Breast Cancer Cells, Induces the Adaptive Response, and Can Be Expressed
Authors: Kozhina Ekaterina A. , Ershova Elizaveta S. , Okorokova Natalya A. , Veiko Vladimir P. , Malinovskaya Elena M. , Sergeeva Vasilina A. , Konkova Marina S. , Kutsev Serguey I. , Veiko Nataly N. , Kostyuk Svetlana V. 
Published: November 2019

Background.Oxidized human DNA or plasmid DNAs containing human ribosomal genes can easily penetrate into the breast cancer cells MCF7 and stimulate the adaptive response induction. Plasmid DNA containing a CMV promoter, geneEGFP, and the insertion of the human ribosomal genes can be expressed. A hypothesis is proposed: these features of the ribosomal DNA are due to the presence of dGn motifs that are prone to oxidize.Methods.Cells of MCF7 line were cultured with plasmids which contained a CMV promoter and gene of fluorescent protein EGFP.Genetic construction pEGFP-Gn contains pEGFP vector and a small insertion with dG11and dG13motifs that are inclined to oxidation. The accumulation of pEGFP and pEGFP-Gn in MCF7 (qPCR), the levels of ROS in the cells, the content of 8-oxodG in plasmids and cellular DNA (flow cytometry, immunoassay, and fluorescent microscopy), the expression ofNOX4andEGFP, the localization of NOX4 and EGFP in MCF7 (qPCR, flow cytometry, and fluorescent microscopy), and the levels of the cell DNA damage (comet assay) were analyzed.Results. (dG)n insertions in the plasmid pEGFP increase the levels of ROS, the cell DNA oxidation and DNA damage, and the level of transfection of plasmid into the MCF7 cells. NOX4 participates in the oxidation of pEGFP-Gn and pEGFP. The expression ofEGFPgene in MCF7 is significantly increased in case of pEGFP-Gn. Stimulation of ROS synthesis (H2O240 μM or 10 cGy IR) increases the level of expression ofEGFP.Conclusions.GC-rich DNA fragments containing dGn motifs that are inclined to oxidation penetrate into MCF7 cancer cells, stimulate the adaptive response, and can be expressed. This property of GC-rich cell-free DNA should be considered and/or could potentially be used in therapy of tumors.
Alien spiders: First record of Loxosceles gaucho Gertsch, 1967 (Araneae: Sicariidae) in the Amazon region, Brazil
Authors: Almeida Marlus Queiroz , Alves-Oliveira João Rafael , Mendes Diego Matheus De Mello , Sobral Rafael , Silva-Neto Alberto Moreira da , Morais José Wellington de 
Published: November 2019

We present the first record for Loxosceles gaucho Gertsch, 1967 in the Amazonian region of Brazil. Four males, fifteen females and forty-nine immatures were collected in different places in Manaus, Amazonas, Brazil. This is the third species of Loxosceles reported in the Amazon region along with L. amazonica Gertsch, 1967, and L. similis Moenkhaus, 1898. This is the first record of an invasive species of a venomous animal in the state of Amazonas, Brazil, which is noteworthy due to its synanthropic habit, which increases the risk to the local population.
Nassarius foveolatus (Gastropoda, Nassariidae), a new record of an exotic species in Brazil
Authors: Gernet Marcos De Vasconcellos , Belz Carlos Eduardo , Baggio Rafael Antunes , Birckolz Carlos João , Santos Elizângela Da Veiga , Simone Luiz Ricardo Lopes de , Abatte Daniel , Metri Rafael 
Published: November 2019

Exotic species are those that occur in an area beyond their natural limit and they are considered invasive when they cause harm to the economy, environment, or human health. In coastal environments, ballast water and inlays on the hull and other parts of vessels are the main ways of introducing invasive aquatic alien species. Nassarius foveolatus (Dunker, 1847) is native from the Central and East Indian Ocean to the East China Sea. The first specimens (empty shells) of N. foveolatus were collected manually on November 11, 2017 on the Rocio footbridge, located in the Paranaguá Estuarine Complex, on the coast of the State of Paraná, southern Brazil. Posteriorly, live specimens were collected in other localities of this bay. It is already possible to infer that the specimens of N. foveolatus occur together with the native specimens of N. vibex (Say, 1822), having the same niche. As previously only N. vibex existed in that place, at least a displacement of this native species has been occurred. However, certainly future ecological studies may confirm this displacement and additional consequences to the local ecosystem, as nassariids can be predators and scavengers. Control procedures should be also greatly implemented.
Natural diet of Ocypode quadrata (Fabricius, 1787) (Crustacea, Decapoda, Brachyura) from the Northern Coast of São Paulo, Brazil
Authors: Gomes Tassia Turini , Gheler-Costa Carla , Rinaldi Caroline Anacleto , Santana William 
Published: November 2019

Decapod crustaceans have a wide variety of feeding habits, alternating among herbivory, predation, saprophagy, and filtration. The occupation of various trophic positions in the food web is a key feature in the evolution of the group. Thus, we analyzed the natural diet of the crab Ocypode quadrata (Fabricius, 1787), commonly known as the ghost crab and typically found on sandy beaches. The crabs were collected in the region of Ubatuba, between July 2016 and May 2017. The individuals were dissected in a laboratory, and each stomach was weighed and visually assessed in relation to the degree of repletion. After identification and classification, the items found were grouped for the analysis. In analyzing the stomach contents of Ocypode quadrata, 12 items were found: sand, Insecta, Hymenoptera, Hemiptera, Coleoptera, Crustacea, and other unidentified Arthropoda. We also found poriferans, Actnopterygii bones, plant pieces, non-organic material (plastic), and other non-identified materials. In the statistical analyzes, we observed seasonal differences in the composition of the diet, mostly related to the frequency of items consumed between dry and rainy seasons; the rainy season provided a greater diversity of items. Due to the great trophic spectrum of this species and tendency to feed on a wide range of items, O. quadrata is considered a generalist species, adapting according to the availability of prey in the wild.
Comparison of estradiol benzoate and cypionate in Girolando cows submitted to a timed artificial insemination
Authors: Silva Otávia Reis , Mello Marco Roberto Bourg de , Silenciato Lara Nogueira , Ferreira Joaquim Esquerdo , Couto Samuel Rodrigues Bonamichi do , Fajardo Rosane Scatamburlo Lizieire , Resende Osvaldo De Almeida 
Published: November 2019

The various studies on the manipulation of the estrous cycle culminated in the development of estrous and ovulation synchronization protocols, to the point of defining an optimum moment for artificial insemination. The induction of ovulation is a primordial part for the determination of the moment of the TAI, so to study which hormones and how they behave allows a better understanding of this physiological process and, to manipulate it efficiently. The aim of the present study was to compare the use of estradiol benzoate (EB) and cypionate (ECP) as ovulation inducer in Girolando cows submitted to Timed Artificial Insemination (TAI). Ovulations of 108 Girolando cows were synchronized and the initial day of treatment, recorded as D0, which was when the animals received an intravaginal device containing 1g of progestogen and an intramuscular application (im) of 2mg of EB. After 8 days (D8), the device was removed and 500μg of cloprostenol (im) was administered to all females. Then, the animals were randomly divided into two treatments: BG Group (n = 52) and CG Group (n = 56). The CG animals received 1mg of ECP (im) as the ovulation inducer at the time of device removal, while BG Group cows received 1mg of EB (im) 24 h later (D9). TAI occurred at D10. After device removal, ultrasound evaluations were performed every 12 h up to ovulation. The following reproductive parameters were evaluated: interval from intravaginal device removal to ovulation - IDO (hours); interval from ovulation to TAI-IOT (hours); diameter of the largest follicle at intravaginal device removal (mm); maximum diameter of the DF (mm) at D10; the dominant follicle growth rate (mm/day); synchronization rate - SR (%); ovulation rate - OR (%) and PR - pregnancy rate (%). Only the ovulation rate presented a statistical difference (p<0.05). In conclusion, despite the ovulation rate difference, both estradiol esters administered were effective and presented similar pregnancy rates in Girolando cows submitted to TAI.
Comparison of a video versus paper questionnaire on functional limitation in lower limb osteoarthritis
Authors: Dubouis L. , Ngueyon-Sime W. , Peter W. , Vallata A. , Epstein J. , Rat A-C. , Agrinier N. , Terwee C. B. , Guillemin F. 
Published: November 2019

Abstract

Introduction
The video Animated Activity Questionnaire (AAQ) was developed to assess the impact of lower limb osteoarthritis (OA) on daily functional activities. The objective of the study was to compare the video and the HOOS/KOOS paper questionnaires and to assess the effect of order of administration.

Material and methods
Patients recruited in the KHOALA cohort were randomized in two groups: AAQ questionnaire first (AAQ-first group) and HOOS (hip)/KOOS (knee) questionnaire first (H/KOOS-first group). Within group differences between AAQ and HOOS/KOOS scores were compared using a Student t-test. The Spearman correlation coefficient between AAQ score and HOOS/KOOS score was calculated in each group then compared, using Fisher z-transformation.

Results
Among 200 randomized patients, 188 (65.8 years, 66.0% women) completed the questionnaires: 99 in the AAQ-first group and 89 in the H/KOOS-first group. The AAQ score was 85.9 (SD: 13.7) in the AAQ-first versus 87.8 (SD: 13.1) in the H/KOOS-first group (p = 0.34). The H/KOOS score was 72.5 (SD: 21.2) in the AAQ-first versus 73.5 (SD: 18.4) in the H/KOOS-first group (p = 0.71). The Spearman correlation coefficient between AAQ and H/KOOS in the AAQ-first was 0.84[0.77–0.89] and 0.73[0.61–0.81] in H/KOOS-first group. These correlations differed between groups significantly (p = 0.02).

Conclusion
This study found video AAQ and paper HOOS/KOOS questionnaire highly correlated, with a moderate but significant effect of order administration of video and paper questionnaires evidencing a stronger correlation when the videos were viewed first.
Introduction the revolving scarf osteotomy for treating severe hallux valgus with an increased distal metatarsal articular angle: a retrospective cohort study
Authors: Wang Xinwen , Wen Qian , Li Yi , Liu Cheng , Zhao Kai , Zhao Hongmou , Liang Xiaojun 
Published: November 2019

AbstractBackgroundHallux valgus(HV) with an increased distal metatarsal articular angle (DMAA) is one of the most common foot deformities among adults. Double metatarsal osteotomy (DMO) is effective in treating severe HV deformity with an increased DMAA. However, this technique presents the risk of avascular necrosis (AVN) of the metatarsal head and transfer metatarsalgia due to shortening of the first metatarsal. The aim of this study was to introduce a surgical procedure defined as revolving scarf osteotomy (RSO) and compare the clinical and radiological results of RSO and DMO performed for treating severe HV with an increased DMAA.MethodsFirst metatarsal osteotomies and Akin osteotomy were performed in 56 patients (62 ft) with severe HV with an increased DMAA in Honghui Hospital from January 2015 to December 2017. RSO was performed in 32 ft and DMO was performed in 30 ft. The Akin osteotomy was performed in both groups. The American Orthopedic Foot and Ankle Society (AOFAS) score, visual analogue scale (VAS) score, the hallux valgus angle (HVA), intermetatarsal angle (IMA), DMAA, and first metatarsal length (FML) and the rates of complications were compared preoperatively and postoperatively in the two groups.ResultsThe mean AOFAS score, VAS score, HVA, IMA, and DMAA showed significant improvements in both groups after surgery, but with no significant differences between the two groups. The postoperative FML was significantly larger in the RSO group than in the DMO group (p < 0.001). One of the 30 ft (3.3%) in the DMO group exhibited transfer metatarsalgia at 12 months postoperatively, while another foot (3.3%) in same group had avascular necrosis of the metatarsal head. One of the 30 ft (3.1%) in the RSO group had hallux varus.ConclusionsNo differences in the clinical and radiographic results were observed between the two groups with severe HV and an increased DMAA. However, RSO does not cause shortening of the metatarsal and AVN of the metatarsal head. A long-term, randomized, controlled prospective study with a larger sample would provide higher-level evidence for confirming the clinical efficacy and safety of RSO.
Technology-assisted rehabilitation following total knee or hip replacement for people with osteoarthritis: a systematic review and meta-analysis
Authors: Wang Xia , Hunter David J. , Vesentini Giovana , Pozzobon Daniel , Ferreira Manuela L. 
Published: November 2019

Abstract

Background
To evaluate the effectiveness and safety of technology-assisted rehabilitation following total hip/knee replacement (THR/TKR).

Methods
Six electronic databases were searched without language or time restrictions for relevant studies: MEDLINE, EMBASE, Cochrane Library, CINAHL, SPORTDiscus, Physiotherapy Evidence Database (PEDro); from inception to November 7th, 2018. Two reviewers independently applied inclusion criteria to select eligible randomised controlled trials (RCTs) that investigated the effectiveness of technology-based interventions, compared with usual care or no intervention for people undergoing THR/TKR. Two reviewers independently extracted trial details (e.g. patients’ profile, intervention, outcomes, attrition and adverse events). Study methodological quality was assessed using the PEDro scale. Quality of evidence was critically appraised using the Grading of Recommendations, Assessment, Development and Evaluation approach.

Results
We identified 21 eligible studies assessing telerehabilitation, game- or web-based therapy. There were 17 studies (N = 2188) in post-TKR rehabilitation and 4 studies (N = 783) in post-THR rehabilitation. Compared to usual care, technology-based intervention was more effective in reducing pain (mean difference (MD): − 0.25; 95% confidence interval (CI): − 0.48, − 0.02; moderate evidence) and improving function measured with the timed up-and-go test (MD: -7.03; 95% CI: − 11.18, − 2.88) in people undergoing TKR. No between-group differences were observed in rates of hospital readmissions or treatment-related adverse events (AEs) in those studies.

Conclusion
There is moderate-quality of evidence showed technology-assisted rehabilitation, in particular, telerehabilitation, results in a statistically significant improvement in pain; and low-quality of evidence for the improvement in functional mobility in people undergoing TKR. The effects were however too small to be clinically significant. For THR, there is very limited low-quality evidence shows no significant effects.
Exosomes derived from statin-modified bone marrow dendritic cells increase thymus-derived natural regulatory T cells in experimental autoimmune myasthenia gravis
Authors: Zhang Peng , Liu Ru-Tao , Du Tong , Yang Chun-Lin , Liu Yu-Dong , Ge Meng-Ru , Zhang Min , Li Xiao-Li , Li Heng , Dou Ying-Chun , Duan Rui-Sheng 
Published: November 2019

Abstract

Background
The thymus plays an essential role in the pathogenesis of myasthenia gravis (MG). In patients with MG, natural regulatory T cells (nTreg), a subpopulation of T cells that maintain tolerance to self-antigens, are severely impaired in the thymuses. In our previous study, upregulated nTreg cells were observed in the thymuses of rats in experimental autoimmune myasthenia gravis after treatment with exosomes derived from statin-modified dendritic cells (statin-Dex).

Methods
We evaluated the effects of exosomes on surface co-stimulation markers and Aire expression of different kinds of thymic stromal cells, including cTEC, mTEC, and tDCs, in EAMG rats. The isolated exosomes were examined by western blot and DLS. Immunofluorescence was used to track the exosomes in the thymus. Flow cytometry and western blot were used to analyze the expression of co-stimulatory molecules and Aire in vivo and in vitro.

Results
We confirmed the effects of statin-Dex in inducing Foxp3+ nTreg cells and found that both statin-Dex and DMSO-Dex could upregulate CD40 but only statin-Dex increased Aire expression in thymic stromal cells in vivo. Furthermore, we found that the role of statin-Dex and DMSO-Dex in the induction of Foxp3+ nTreg cells was dependent on epithelial cells in vitro.

Conclusions
We demonstrated that statin-Dex increased expression of Aire in the thymus, which may further promote the Foxp3 expression in the thymus. These findings may provide a new strategy for the treatment of myasthenia gravis.
Development, progress and future prospects in cryobiotechnology of Lilium spp.
Authors: Li Jing-Wei , Zhang Xiao-Chen , Wang Min-Rui , Bi Wen-Lu , Faisal M. , da Silva Jaime A. Teixeira , Volk Gayle M. , Wang Qiao-Chun 
Published: November 2019

Abstract
Lilium is one of the most popular flower crops worldwide, and some species are also used as vegetables and medicines. The availability of and easy access to diverse Lilium genetic resources are essential for plant genetic improvements. Cryopreservation is currently considered as an ideal means for the long-term preservation of plant germplasm. Over the last two decades, great efforts have been exerted in studies of Lilium cryopreservation and progress has been made in the successful cryopreservation of pollen, seeds and shoot tips in Lilium. Genes that exist in Lilium, including those that regulate flower shape, color and size, and that are resistant to cold stress and diseases caused by fungi and viruses, provide a rich source of valuable genetic resources for breeding programs to create novel cultivars required by the global floriculture and ornamental markets. Successful cryopreservation of Lilium spp. is a way to preserve these valuable genes. The present study provides updated and comprehensive information about the development of techniques that have advanced Lilium cryopreservation. Further ideas are proposed to better direct future studies on Lilium cryobiotechnology.
CRY arrests Cop1 to regulate circadian rhythms in mammals
Authors: Lee Choogon 
Published: November 2019

Abstract
Cryptochromes (CRYs) are UVA and blue light photoreceptors present in all major evolutionary lineages ranging from cyanobacteria to plants and animals, including mammals. In plants, blue light activates CRYs to induce photomorphogenesis by inhibiting the CRL4Cop1 E3 ligase complex which regulates the degradation of critical transcription factors involved in plant development and growth. However, in mammals, CRYs do not physically interact with Cop1, and of course mammals are not photomorphogenic, leading to the belief that the CRY–Cop1 axis is not conserved in mammals. This belief was recently overturned by Rizzini et al., who showed that although mammalian CRYs do not inhibit Cop1 activity in a light-dependent manner, they antagonize Cop1 activity by displacing Cop1 from CRL4 E3 ligase complex. Because CRYs oscillate, they act in a circadian manner resulting in daily oscillations in Cop1 substrates and the downstream pathways that they regulate. The conserved antagonism of Cop1 by CRY indicates that the CRY–Cop1 axis has an ancient origin, and was repurposed by evolution to regulate photomorphogenesis in plants and circadian rhythms in mammals.
iPBAvizu: a PyMOL plugin for an efficient 3D protein structure superimposition approach
Authors: Faure Guilhem , Joseph Agnel Praveen , Craveur Pierrick , Narwani Tarun J. , Srinivasan Narayanaswamy , Gelly Jean-Christophe , Rebehmed Joseph , de Brevern Alexandre G. 
Published: November 2019

Abstract

Background
Protein 3D structure is the support of its function. Comparison of 3D protein structures provides insight on their evolution and their functional specificities and can be done efficiently via protein structure superimposition analysis. Multiple approaches have been developed to perform such task and are often based on structural superimposition deduced from sequence alignment, which does not take into account structural features. Our methodology is based on the use of a Structural Alphabet (SA), i.e. a library of 3D local protein prototypes able to approximate protein backbone. The interest of a SA is to translate into 1D sequences into the 3D structures.

Results
We used Protein blocks (PB), a widely used SA consisting of 16 prototypes, each representing a conformation of the pentapeptide skeleton defined in terms of dihedral angles. Proteins are described using PB from which we have previously developed a sequence alignment procedure based on dynamic programming with a dedicated PB Substitution Matrix. We improved the procedure with a specific two-step search: (i) very similar regions are selected using very high weights and aligned, and (ii) the alignment is completed (if possible) with less stringent parameters. Our approach, iPBA, has shown to perform better than other available tools in benchmark tests. To facilitate the usage of iPBA, we designed and implemented iPBAvizu, a plugin for PyMOL that allows users to run iPBA in an easy way and analyse protein superimpositions.

Conclusions
iPBAvizu is an implementation of iPBA within the well-known and widely used PyMOL software. iPBAvizu enables to generate iPBA alignments, create and interactively explore structural superimposition, and assess the quality of the protein alignments.