bluuebunny/crossref_metadata_2025_split huggingface dataset:
How human intervention and climate change shaped the fate of the Northern Bald Ibis from ancient Egypt to the presence: an interdisciplinary approach to extinction and recovery of an iconic bird species
Authors: Fritz Johannes , Janák Jiří 
Published: November 2020

AbstractOnce widespread around the Mediterranean, the Northern Bald Ibis (Geronticus eremita) became one of the rarest birds in the world. We trace the history of this species through different epochs to the present. A particular focus is on its life and disappearance in ancient Egypt, where it attained the greatest mythological significance as a hieroglyphic sign for ‘blessed ancestor spirits’, and on modern endeavours to rewild and restore the species. The close association of the Northern Bald Ibis with human culture in ancient Egypt, as in other regions, is caused by primarily two reasons, the characteristic appearance and behaviour, as well as the need for open foraging areas. In consequence, a mutualistic relationship between humans and birds was formed in some cultures. The benefit for the Northern Bald Ibis was mainly the availability of feeding habitats, which were cleared by humans for farming or grazing and might have contributed to the spread of the species. The benefit to people was primarily cultural and mythological, whereby the bird was worshiped in ancient Egypt and in Muslim cultures, while Christian cultures in Europe rather regarded it as bad omen or nuisance, like any black bird species. Another benefit was profane in nature, the species was also hunted for food, mainly in Europe. But alike many other species, proximity to humans also carried a high risk for the Northern Bald Ibis. We discuss various kinds of human impacts that were driving causes for the extinction of the species in almost all regions. However, the historical disappearance of populations also correlates markedly with changes in climate, especially in ancient Egypt and the Middle Ages. This fact has important implications for current conservation efforts, especially since international action plans for the Northern Bald Ibis have taken little account of climate change effects so far. The Northern Bald Ibis is an outstanding example of how an interdisciplinary cultural-historical and natural-scientific approach significantly promotes the interpretation of historical evidence as well as the implementation of current rewilding and restoration efforts.
PLD2-phosphatidic acid recruit ESCRT-I to late endosomes for exosome biogenesis
Authors: Egea-Jimenez Antonio Luis , Audebert Stéphane , Castro-Cruz Monica , Borg Jean-Paul , David Guido , Camoin Luc , Zimmermann Pascale 
Published: November 2020

AbstractIt is important to understand the biogenesis of exosomes, extracellular vesicles of endosomal origin controlling cell-to-cell communication. We previously reported that Phospholipase D2 (PLD2) supports late endosome (LE) budding and the biogenesis of syntenin-dependent exosomes. Here, we reveal that PLD2 has a broader generic effect on exosome production. Combining gain- and loss-of-function experiments, proteomics, microscopy and lipid-binding studies with reconstituted liposomes mimicking LE, we show that: (i) PLD2 activity controls the recruitment of MVB12B to LE and the exosomal secretion of ESCRT-I; (ii) loss-of-MVB12B phenocopies loss-of-PLD2, similarly affecting LE budding, the number of exosomes released and exosome loading with cargo; (iii) MVB12B MABP domain directly interacts with phosphatidic acid, the product of PLD2. We therefore propose that PLD2 and phosphatidic acid support ESCRT-I recruitment to LE for the formation of exosomes. This work highlights a major unsuspected piece of the molecular framework supporting LE and exosome biogenesis.
Selective transport of fluorescent proteins into the phage nucleus
Authors: Nguyen Katrina T. , Sugie Joseph , Khanna Kanika , Egan MacKennon E. , Birkholz Erica A. , Lee Jina , Beierschmitt Christopher , Villa Elizabeth , Pogliano Joe 
Published: November 2020

AbstractUpon infection ofPseudomonascells, jumbo phages 201Φ2-1, ΦPA3, and ΦKZ assemble a phage nucleus. Viral DNA is enclosed within the phage-encoded proteinaceous shell along with proteins associated with DNA replication, recombination and transcription. Ribosomes and proteins involved in metabolic processes are excluded from the nucleus. RNA synthesis occurs inside the phage nucleus and messenger RNA is presumably transported into the cytoplasm to be translated. Newly synthesized proteins either remain in the cytoplasm or specifically translocate into the nucleus. The molecular mechanisms governing selective protein sorting and nuclear import in these phage infection systems are currently unclear. To gain insight into this process, we studied the localization of five reporter fluorescent proteins (GFP+, sfGFP, GFPmut1, mCherry, CFP). During infection with ΦPA3 or 201Φ2-1, all five fluorescent proteins were excluded from the nucleus as expected; however, we have discovered an anomaly with the ΦKZ nuclear transport system. The fluorescent protein GFPmut1, expressed by itself, was transported into the ΦKZ phage nucleus. We identified the amino acid residues on the surface of GFPmut1 required for nuclear targeting. Fusing GFPmut1 to any protein, including proteins that normally reside in the cytoplasm, resulted in transport of the fusion into the nucleus. Although the mechanism of transport is still unknown, we demonstrate that GFPmut1 is a useful tool that can be used for fluorescent labelling and targeting of proteins into the ΦKZ phage nucleus.
Direct information estimation from cryo-EM Movies with CARYON
Authors: Ramlaul Kailash , Burt Alister , de Martín Garrido Natàlia , MacDonald James T. , Palmer Colin M. , Jakobi Arjen , Aylett Christopher H. S. 
Published: November 2020

AbstractWhile cryo-EM with modern direct electron detectors has proven incredibly powerful, becoming a dominant technique in structural biology, the analysis of cryo-EM images is significantly complicated by their exceptionally low signal-to-noise ratio, limiting the accuracy of the parameterisation of the physical models required for successful classification and reconstruction.Micrographs from modern direct electron detectors are typically collected as dose-fractionated multi-frame movies to allow the recording of separated individual electron impacts. These detectors improve electron detection and allow for both inter-frame motion correction, and dose-dependent image filtering, lessening the overall impact of effects deleterious to the recovery of high-resolution information.In this study we measured the information content at each spatial frequency in cryo-EM movies as it accrues during the course of an exposure. We show that, as well as correction for motion and radiation damage, the use of the information within movies allows substantially improved direct estimation of the remaining key image parameters required for accurate 3D reconstruction: the image CTF and spectral SNR.We are developing “CARYON” {insert contrived acronym here}, as a LAFTER-family filter for cryo-EM movies based upon such measurements. CARYON is intended to provide the best parameter estimation and filtration possible for a single complete, or large sub-section from a, movie micrograph without the use of a previously refined density. We demonstrate its utility in both single-particle and tomographic cryo-EM data processing.
Broad-spectrum antiviral activity of 3D8, a nucleic acid-hydrolyzing single chain variable fragment (scFv), targeting SARS-CoV-2 and multiple coronavirusesin vitro
Authors: Lee Gunsup , Budhathoki Shailesh , Lee Geum-Young , Oh Kwang-ji , Ham Yeon Kyoung , Kim Young Jun , Lim Ye Rin , Hoang Phuong Thi , Lee Yongjun , Lim Seok-Won , Kim Jun-Mo , Cho Seungchan , Kim Tai-Hyun , Song Jin-Won , Lee Sukchan , Kim Won-Keun 
Published: November 2020

AbstractBackgroundThe current pandemic, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is responsible for the etiology of Coronavirus-induced disease 19 (COVID-19) and poses a critical public health threat worldwide. Effective therapeutics and vaccines against multiple coronaviruses remain unavailable. Single chain variable fragment (scFv), a recombinant antibody exhibits broad-spectrum antiviral activity against DNA and RNA viruses owing to its nucleic acid-hydrolyzing property.ObjectiveThis study is aimed to investigate an antiviral activity of 3D8 scFv against SARS-CoV-2 and other coronaviruses.Methods3D8, a recombinant scFv antibody was evaluated for antiviral activity against SARS-CoV-2, HCoV-OC43 and PEDV in Vero E6 cell cultures. Viral growth was quantified with quantitative RT-qPCR and plaque assay. Nucleic acid hydrolyzing activity of 3D8 was assessed through abzyme assays ofin vitroviral transcripts and cell viability was determined by MTT assay.Results3D8 inhibited the replication of SARS-CoV-2, human coronavirus OC43 (HCoV-OC43), and porcine epidemic diarrhea virus (PEDV). Our results revealed the prophylactic and therapeutic effects of 3D8 scFv against SARS-CoV-2 in Vero E6 cells. Immunoblot and plaque assays showed the reduction of coronavirus nucleoproteins and infectious particles respectively in 3D8 scFv-treated cells.ConclusionsThis data demonstrates the broad-spectrum antiviral activity of 3D8 against SARS-CoV-2 and other coronaviruses. Thus, it could be considered a potential antiviral countermeasure against SARS-CoV-2 and zoonotic coronaviruses.
Electron momentum density of hexagonal Zn studied by high-resolution Compton scattering
Authors: Brancewicz Marek , Andrejczuk Andrzej , Żukowski Eugeniusz , Dobrzyński Ludwik , Sakurai Yoshiharu , Itou Masayoshi , Pylak Maciej 
Published: November 2020

High-resolution (0.12 a.u.) electron momentum density projections (Compton profiles) of a hexagonal Zn single crystal have been measured along five high-symmetry directions in reciprocal space. The experiment was performed with the use of 115.6 keV synchrotron radiation on the BL08W station at SPring-8. The quality of the measured Compton profiles is significantly better than that of previous medium- and high-resolution data. The experimental data were compared with the corresponding theoretical Korringa–Kohn–Rostoker (KKR) and density functional theory (DFT) calculations. Some minor and major differences between the two theoretical band-structure calculations have been observed. However, the good quality experimental results indicate their better agreement with DFT.
Benefits and challenges of developing an eco-social orientation: implications for marketing practice
Authors: Gabler Colin Bauer , Landers V. Myles , Richey R. Glenn 
Published: November 2020

PurposeSocial and environmental actors have been added to the stakeholder balancing act required of organizations in today’s competitive marketplace. To address this, the firms may create orientations to convey their strategic priorities. The purpose of this paper is to explore how different levels of environmental orientation (EO) and social orientation (SO) influence firm outcomes. This paper leverages logic from the too-much-of-a-good-thing effect to predict that firms must strategically align their resources with performance goals to optimize these resources.Design/methodology/approachThe paper outlines a step-by-step guide that combines latent moderated structural equation modeling with response surface analysis to estimate curvilinear relationships. The approach leverages the benefits of both techniques to produce precise results and more nuanced – and meaningful – implications.FindingsThe procedure is effective in uncovering a curvilinear relationship between the indicator variables. Specifically, firms optimize environmental performance when EO is higher than SO. The opposite is the case for the outcome of social value and manager trust. Economic performance is highest when both indicators are simultaneously high.Research limitations/implicationsThis study tests the relationships between social and EOs and performance. As such, the exploratory data in this study are US-centric and self-assessment in design. These limitations open the door to confirmatory studies using objective outcome data and cross-cultural comparisons. Such studies should embrace more variables and examine potential moderating influences. Most importantly, future research should work to expand and verify the development of the eco-SO construct presented here, as the dynamics of these relationships have been overlooked in most social responsibility and sustainability research. Future studies should adopt this construct into extant models and also consider the dynamics and inter-organizational fit for partner firms with competing orientations.Practical implicationsFor managers, the results show that conveying an environmental or social outcome has unique benefits to the firm. Further, there is an incentive to try and do both simultaneously. However, there is a critical point where the effects taper off, which can guide managers as they implement social and/or environmental strategies.Social implicationsThe research questions ask if a company can simultaneously deliver: economic value to shareholders, environmental value to the planet and social value to consumers while maintaining the trust of its managers. The results generally support that to fully serve one group, a firm must underserve another.Originality/valueThe study introduces SO as a valid construct to mirror EO and then models their interaction in three-dimensional space to present an optimal firm strategy.
“Us” to co-create value and hate “them”: examining the interplay of consumer-brand identification, peer identification, value co-creation among consumers, competitor brand hate and individualism
Authors: Itani Omar S. 
Published: November 2020

Purpose
The purpose of this paper is to understand the role of identity-based relationships, customer brand identification and peer identification, in driving customer outcomes including customer experiential hedonic value, social influence and repurchase intentions through the effects on value co-creation among customers and competitor brand hate, while taking into consideration the moderating impact of individualism.

Design/methodology/approach
The study integrates social identity theory, identity-based marketing perspective and self-construal theory to develop relationships. The data comprises a web-based survey of customers in the USA and was analyzed using structural equation modeling.

Findings
Customer brand identification and peer identification are drivers of value co-creation among customers, which leads to favorable outcomes at the customer and brand levels. Customer brand identification drives customers to hate competing brands, which, in turn, motivates customers to exert social influence in favor of their brand and to hold additional repurchase intentions. Customer brand identification and peer identification play different roles in motivating customers to co-create value with their fellows and drive customers to feel hatred toward competing brands contingent on customer individualism.

Research limitations/implications
Customer brand identification and peer identification play different roles in engaging customers in value co-creation with their peers and competing brands have with their rivals. Individualism self-construal holds a dual role when interacting with customer identification. The study fills multiple gaps in the literature by examining additional effects of customer brand identification and peer identification and exploring a relatively new dimension of the value co-creation process, as well as the role of customers in the competition between brands.

Practical implications
Brands need to view customers who identify with them as socially active customers capable of participating in value co-creation with other customers and engaging in the rivalry faced by the brands. Moreover, brands are required to build and nurture relationships that are based on social identification to encourage customer brand identification and peer identification which results in favorable customer and business outcomes.

Originality/value
This study examines the effects of two forms of customer identification on value co-creation between customers and competitor brand hate. In addition, it identifies the dual moderating role of customer individualism on the effects of both social identification forms. The study fills multiple gaps in the literature by understanding new aspects of customer identification, value co-creation and brand hate.
Demonstrating the motivational scale for commitments toward teachers’ turnover intentions using self-determination theory: a case of higher education institutions in Pakistan
Authors: Maryam Sayeda Zeenat , Ali Fouzia , Rizvi Mehvish , Farooq Sadia 
Published: November 2020

PurposeThis paper aims to demonstrate the turnover intentions (TIs) among the academic faculty of higher education institutions (HEIs) in Pakistan through their motivation and commitment toward the concerned institution. It inspects the relationship of dimensions of motivation including an absence of motivation (AM), extrinsic motivation (ExM) and intrinsic motivation (InM) with the dimensions of commitment including affective commitment (AC), continuous commitment (CC) and normative commitment (NC) and finally the academic's TIs.Design/methodology/approachThe paper used the self-determination theory (SDT), the theory of organizational commitment (OC) and the theory of planned behavior (TPB) for its proposed model. A sample of 500 academics, working under higher education commission recognized public and private universities in Punjab Pakistan. A structural equation modeling (SEM) conducted for analysis using an algorithm, bootstrapping and blindfolding techniques.FindingsThe outcomes of the research are attention-grabbing, as no direct relationship has been observed between the dimensions of motivation and TIs except InM. The dimensions of commitments are mediating the relationship between motivation and TIs with high significance value. The results reveal a significant adverse effect on TIs through two dimensions of commitment.Research limitations/implicationsAs it is the case of a developing country like Pakistan therefore the finding cannot generalize to developed countries.Practical implicationsThe findings of this research may lead the policymakers and practitioners of HEIs and controlling body to retain their competent teachers.Social implicationsThis research can help the private sector to develop strategies about the retention of their competent teachers within the institutions that not just value the institution but also will be much beneficial for the students and society.Originality/valueThis paper is identifying how motivation is related to TIs and the role of commitment in it at HEIs. Still, no research has been conducted considering this avenue of SDT, OC and TPB
Micro, small and medium enterprises’ competitiveness and micro-takāfuladoption
Authors: Md Husin Maizaitulaidawati , Haron Razali 
Published: November 2020

PurposeThis paper aims to attempt to first examine the competitiveness of micro, small and medium-sized enterprises (MSMEs) in the logistics industry; second, to identify the MSMEs’ perception towardstakāful(Islamic insurance); third, to recognise the challenges in the adoption oftakāful; and fourth, to suggest strategies to enhance the micro-takāfulpenetration rate.Design/methodology/approachThe SWOT (strengths, weaknesses, opportunities and threats) analysis was used to measure the MSMEs’ competitiveness. Interview sessions were conducted with 13 owners of MSMEs in the logistics industry from the period November 2018 until January 2019 in Selangor, Malaysia.FindingsThe SWOT analysis identified several strengths (e.g. advanced infrastructure, rising number of new entrants and contribution to the local economy), weaknesses (e.g. lack of digital culture and training and a dearth of expertise), opportunities (e.g. supportive government initiatives and evolution of the mobile internet) and threats (e.g. changing customer expectations and limited financing facilities). The MSMEs’ perception towards micro-takāfuland challenges in the adoption oftakāfulwere also identified.Research limitations/implicationsThis paper provides an understanding of the MSMEs’ perception towards micro-takāfulproducts, sheds light on the challenges faced by MSME owners in protecting their businesses from risk exposures and offers strategies to enhance the micro-takāfulpenetration rate. This study, however, is limited to Malaysia’s experience.Practical implicationsThe identification of MSMEs’ SWOT will be useful for these businesses as it provides solid information that can be used to improve business performance while also seekingtakāfulprotection. This paper, other than serving as a guideline for stakeholders in the logistics industry to have a better understanding of their business environment, may also provide useful insights to practitioners and policymakers.Originality/valueThis paper integrates the SWOT analysis into a study on business risk exposure andtakāfulprotection from the MSMEs’ perspective. Hence, the findings could broaden available knowledge on MSMEs, especially for businesses in the logistics industry. The knowledge may also facilitate matters fortakāfuloperators interested in tapping into the market.
The Covid-19 pandemic and entrepreneurship: some reflections
Authors: Liñán Francisco , Jaén Inmaculada 
Published: November 2020

PurposeThe Covid-19 pandemic has affected the world in an unprecedented manner. The economic recession in 2020 is expected to be the most serious since the Second World War. This study aims to analyse the likely consequences of the Covid-19 pandemic crisis on entrepreneurship and new venture activity.Design/methodology/approachThe paper focuses first on the major shocks caused by the pandemic that may affect entrepreneurial activity. To do so, the authors briefly review the literature about the previous financial crisis and its effects on entrepreneurship. Then, the manuscript means to disentangle how these shocks will impinge on the different stages and motives within the entrepreneurial process.FindingsThis analysis reveals that the consequences of the pandemic for entrepreneurship will be generally damaging, but they may not be so tremendously negative as originally thought. We could initially expect a broad downturn in entrepreneurial activity. Soon after that, however, necessity entrepreneurship is likely to boom. At the same time, but to a lesser extent, high-potential entrepreneurial activity could also be fostered, provided the recovery is quick and there is sufficient support from the environment and institutions.Originality/valueThis is probably one of the first academic reflections on the likely effects of the Covid-19 pandemic on entrepreneurship. It specifically addresses the role of institutions and how they may differently affect necessity and opportunity entrepreneurship. It also suggests interesting related research areas and some basic recommendations on how to help entrepreneurs overcome it.