bluuebunny/crossref_metadata_2025_split huggingface dataset:
Choosing and Doing Wisely: Triage Level I Resuscitation a Possible New Field for Starting Palliative Care and Avoiding Low-Value Care-A nationwide matched-pair retrospective cohort study
Authors: Lin Chih-Yuan , Lee Yue-Chune 
Published: March 2020

Abstract
Background The association between palliative care and life-sustaining treatment for patients following emergency department (ED) resuscitation is unclear.Objective To analyze ED triage level I resuscitation patients the use of palliative care and its life-sustaining treatment usage based on a national representative sample of a population of about 2.3 million.Methods A matched-pair retrospective cohort study was conducted to examine the association between palliative care and the outcome variables using multivariate logistic regression and Kaplan–Meier survival analyses.Participants: Between 2009 and 2013, 336 ED triage level I resuscitation patients received palliative care services (palliative care group) in a universal health insurance scheme. Retrospective cohort control selection was matched using a 1:4 ratio for confounding factors with those who received usual care (usual care group).Main outcomes Outcome variables include numbers of emergency and outpatient department visits, hospitalization days, total medical expenses, life-sustaining treatment utilization, and days of survival after the ED triage level I resuscitation index date.Results The mean survival post-ED triage level I resuscitation days was less than one year, that, fit the end-of-life definition. Palliative care services provided to 15% of ED triage level I resuscitation patients. The palliative care group was more consistently and significantly associated with less life-sustaining treatment use than the usual care group.Conclusion Palliative care decisions correlate with the reduction of life-sustaining treatments for patients receiving triage level I resuscitation. Furthermore, triage level Ⅰ resuscitation status may present a possible new field for starting palliative care intervention and reducing low-value care.
Long noncoding RNA LINC01615 promotes keloid development via sponging miR-590-3p to regulate FGF2 expression
Authors: Zhang Xiaoyu , Xu Yingying , Yamaguchi Kenji , Hu Jinping , Zhang Lianbo , Wang Jianfeng , Tian Jifeng , Chen Wanying 
Published: March 2020

Abstract
Background A keloid is a benign human skin tumor that resulted by fibrous overgrowths during wound healing. Long noncoding RNAs (lncRNAs) are indicated to involve in the development of keloid. However, the role of lncRNA LINC01615 in regulating keloid development and the underlying mechanism are still unknown. Methods The expression levels of LINC01615, miR-590-3p and fibroblast growth factor 2 (FGF2) mRNA were detected by quantitative real-time polymerase chain reaction (qRT-PCR). The protein levels of apoptosis-related proteins, α-smooth muscle actin (α-SMA), collagens and FGF2 were measured by western blot. The effect of LINC01615 on keloid development was assessed by cell proliferation, apoptosis and collagen deposition of keloid fibroblasts which were determined by Cell Counting Kit-8 (CCK-8), flow cytometry assay and the protein levels of collagens, respectively. The relationships between LINC01615 and miR-590-3p, miR-590-3p and FGF2 were predicated by online software and confirmed by dual-luciferase reporter assay and RNA pull-down assay. Results We first found that LINC01615 was upregulated in keloid tissues and fibroblasts, and LINC01615 promoted cell proliferation and collagen deposition and suppressed apoptosis in keloid fibroblasts. LINC01615 targeted miR-590-3p and downregulated miR-590-3p expression, and overexpression of miR-590-3p inhibited the development of keloid. Then, FGF2 was identified as a target of miR-590-3p. LINC01615 facilitated keloid development via regulating FGF2 expression through miR-590-3p. Conclusion Our study demonstrated that LINC01615 contributed to keloid development via the miR-590-3p/FGF2 axis.
Pathological Response Has Survival Benefits for Rectal Cancer following Neoadjuvant Therapy
Authors: Chen Wei-Chih , Kuo Li-Jen , Chen Chia-Che , Wei Po-Li , Huang Yu-Min , Huang Yan-Juin , Tong Yiu-Shun , Chen Ray-Jade 
Published: March 2020

Abstract
Background: Studies reporting the results of associated factors of pathological completed response (PCR) and tumor regression response in patients with rectal cancer following neoadjuvant chemoradiation therapy (nCRT) are inconsistent.
Objective: The purpose of this study was to identify the prognostic factors of tumor response and outcome in rectal cancer patients.
Design: The study was a retrospective analysis.
Settings: The study was conducted in a single large institution in Taiwan.
Patients: Newly diagnosed rectal cancer patients who underwent nCRT followed by surgery from 2010 to 2014 with 5 years of follow-up.
Main Outcome Measures: The primary outcomes were associated factors of pathological completed response and downstaging. The risk factors of survival outcome and disease recurrence were also observed.
Results:
A total of 169 rectal cancer patients were included. The PCR rate was 17.8%, and the downstaging rate was 60.9%. Patients with a histology type of adenocarcinoma associated with PCR, and patients positive for clinical N stage were associated with downstaging. Kaplan-Meier analysis showed the PCR group performed better to a statistically significant level both in overall survival and disease recurrence free survival than the no PCR group (p= 0.033 & 0.025, respectively). Patients with a downstaging response also showed better overall survival benefits and disease recurrence free survival benefits than their counterparts (both p<0.001). After controlling confounding variables, the risk factors of overall survival were downstaging [Hazard Ratio (HR): 0.40, 95% CI: 0.21-0.74], male, abnormal post-nCRT CEA level and abnormal Hb level. In addition, the protective factors of recurrence were downstaging and having received adjuvant chemotherapy.
Limitations: Modest sample size and limited genetic bio-markers information.
Conclusions:
Among rectal cancer patients who received the neoadjuvant therapy, histology type and clinical N stage were associated with PCR and downstaging, respectively. Downstaging was an important protective factor for better overall survival and recurrence free survival.
COVID-19 discharged patients in Hunan, China: correlation between early features and prognosis
Authors: cai yeyu , liu jiayi , Yang Haitao , Chen Taili , Yu Qizhi , Chen Juan , Huang Deng , Chen Zhu , Shang Quanliang , Ma Cong , Chen Xiangyu , Xiao Enhua 
Published: March 2020

Abstract
Purpose To describe the correlation between the clinical, laboratory and radiological findings with hospitalization days in Coronavirus Infected Disease-19 (COVID-19) discharged patients. Method In this multicenter study, we retrospectively identified 153 discharged patients with COVID-19 pneumonia from Jan 16, 2020 to Feb 26, 2020 in Hunan province. Patients were grouped based on the hospitalization days: Group 1 (hospitalization days≦12 days) and Group 2((hospitalization days> 12days). Demographic, clinical characteristics and laboratory findings on admission and the imaging features of the first Chest CT on admission were analyzed. The differences between groups were analyzed using univariate logistic regression to find the impact factors. Results The cohort included 153 discharged patients (85 males and 68 females, with the mean age of 42.32±14.03 years old). 90(58.8%) patients had hospitalization days≦12 and 63(41.2%) patients had hospitalization days>12. 44(48.9%) patients in Group1 and 28(44.4%) in Group 2 had been to Wuhan. In both Group1 and Group2, most common symptoms at onset were fever (62.2%, 60.3%) and cough (33.3%, 50.8%). Cough was occurred more common in Group 2(50.8%) than Group 1(33.3%) with a significant difference (p=0.03). 6(6.7%) patients in Group1 and 10(15.9%) in Group2 had admitting diagnosis as non-pneumonia (p=0.07), some of them occurred mild pneumonia during hospital stay. White blood cell (2.2%, 9.5%) and neutrophil (9.5%) count above normal were more common on in Group 2 (p=0.04, p=0.04). Patients in Group 2 had higher concentration of aspartate aminotransferase (P=0.04) than Group 1. Most of patients had multiple lesions (75.6%, 69.8%) with bilateral distribution (73.3%, 58.7%) in both groups. Mixed ground-glass opacity (GGO) and consolidation appearance were seen in most patients. GGO components > consolidation appearance were more common in Group 1(31.1%) than in Group 2(8.0%) with a significant difference between groups (P<0.01). Patients had cough at onset disease (OR, 0.47; 95%CI, 0.23 to 0.96, p=0.04) and CT represented as GGO components more than consolidation (OR, 4.84; 95%CI, 1.80 to 13.04, p<0.01) were associated with hospitalization days. Conclusions COVID-19 non-pneumonia patients with longer hospitalization days might have the persistent symptoms or pneumonia occurrence after admission. Chest CT could help prompt diagnosis and monitor disease progression, GGO/consolidation >1 in mixed lesions was associated with shorter hospitalization days. Special attention should be paid to the role of radiological features in monitoring disease prognosis.
Rates, predictors and mortality of sepsis-induced acute kidney injury: systematic review and meta-analysis
Authors: Liu Jie feng , Xie Hebin , Ye Ziwei , Wang Lesan 
Published: March 2020

Abstract
Background The incidence and mortality of sepsis-induced acute kidney injury is high. Many studies have explored the causes of sepsis-induced acute kidney injury (AKI). However, its predictors are still uncertain; additionally, a complete overview is missing. A systematic review and a meta-analysis were performed to determine the predisposing factors for sepsis-induced AKI.Method A systematic literature search was performed in the Medline, Embase, Cochrane Library, PubMed and Web of Science databases, with an end date parameter of May 25, 2019. Valid data were retrieved in compliance with the inclusion and exclusion criteria.Result Forty-seven observational studies were included for analysis. A cumulative number of 55911sepsis patients were evaluated. The incidence of AKI caused by septic shock is the highest. 30 possible risk factors were included in the meta-analysis. The results showed that 20 factors were found to be significant. The odds ratio(OR),95% confidence interval (CI) and Prevalence of the most prevalent predisposing factors for sepsis-induced AKI were as the following: Septic shock[2.88(2.36-3.52), 60.47%], Hypertension[1.43(1.20-1.70),38.39%), Diabetes mellitus[1.59(1.47-1.71),27.57%],Abdominal infection[1.44(1.32-1.58),30.87%], Vasopressors use[2.95(1.67-5.22),64.61%],vasoactive drugs use [3.85(1.89-7.87),63.22%], Mechanical ventilation[1.64(1.24-2.16),68.00%), Positive blood culture[1.60(1.35-1.89), 41.19%], Smoke history[1.60(1.09-2.36),43.09%]. Other risk factors include cardiovascular, coronary artery disease, liver disease, unknow infection, diuretics use, ACEI or ARB, gram-negative bacteria and organ transplant.Conclusion A large number of factors are associated with AKI development in sepsis patients. Our review can guide risk-reducing interventions, clinical prediction rules, and patient-specific treatment and management strategies for sepsis-induced acute kidney injury.
Deficiencies in the formation and regulation of anther cuticle and tryphine contribute to male sterility in cotton PGMS line
Authors: Zhang Meng , Liu Ji , Ma Qiang , Qin Yuan , Wang Hantao , Chen Pengyun , Ma Liang , Fu Xiaokang , Zhu Longfu , Wei Hengling , Yu Shuxun 
Published: March 2020

Abstract
Background: Male sterility is a simple and efficient pollination control system that is widely exploited in hybrid breeding. In upland cotton, CCRI9106, a photosensitive genetic male sterile (PGMS) mutant isolated from CCRI040029, was reported of great advantages to cotton heterosis. However, the underlying molecular mechanism of CCRI9106 remains unclear.Results: In this study, light and electron microscopy revealed that the male sterility phenotype of MT was mainly attributed to irregularly exine, lacking tryphine and immature anther cuticle. Based on the cytological characteristics of MT anthers, 18 RNA libraries were constructed from the anthers of MT and WT at tetrad (TTP), late uninucleate (LUNP) and binucleate (BNP) stages of anther development for transcriptomic analysis, therefore revealing a total of 870,4 differentially expressed genes (DEGs). By performing gene expression pattern analysis and protein-protein interaction (PPI) networks construction, we found down-regulation of DEGs in cluster 2, which enriched by the lipid biosynthetic process and the synthesis pathways of several types of secondary metabolites such as terpenoids, flavonoids and steroids, may crucial to the male sterility phenotype of MT, and resulting in the defects of anther cuticle and tryphine, even the irregularly exine. Furthermore, several lipid-related genes together with ABA-related genes and MYB transcription factors were identified as hub genes via weighted gene co-expression network analysis (WGCNA), such as NPC2, LTPG, LTP1, MAKR6, Ghir_D11G032630, Ghir_A01G008890, Ghir_D01G009320, MYB3, MYB7, MYB16 and MYB48. Additionally, the ABA content of MT anthers was reduced across all stage when compared with WT anthers.Conclusions: In summary, we propose that the down-regulation of genes related to the assembly of anther cuticle and tryphine may lead to the male sterile phenotype of MT, and MYB transcription factors together with ABA play key regulatory role in these processes. These findings provide valuable information on the transcriptional level to anther and pollen development, and contribute to elucidate the mechanism of male sterility in upland cotton.
Effect of family doctor contract services on patient perceived quality of primary care in Southern China
Authors: Feng Shanshan , Cheng Aiyun , Luo Zhenni , Xiao Yao , Zhang Luwen 
Published: March 2020

Abstract
Background Family doctor contract service is an important service item in China’s primary care reform. This research was designed to evaluate the impact of the provision of family doctor contract services on the patient-perceived quality of primary care, and therefore give evidence-based policy suggestions. Methods This cross-sectional study of family doctor contract service policy was conducted in three pilot cities in the Pearl River Delta, South China,using a multistage stratified sampling method. The adapted Primary Care Assessment Tool-Adult Edition (PCAT-AS) was used to measure the quality of primary care services. Data was collected through face-to-face interviews held from July to November, 2015. Covariate analysis and multivariate Linear Regression were adopted to explore the effect of contract on the quality of primary care by controlling for the socio-demographic status and health care service utilization factors. Results A total of 828 valid questionnaires were collected. From the interviewees, 453 patients signed the contract (54.7%) and 375 did not (45.3%). Multivariate linear regression showed that patients who received services from contracted family doctors reported higher scores in dimensions of PCAT total score (β=-8.98, P<0.000), first contact-utilization(β=-0.71,P<0.001), first contact-accessibility(β=-1.49, P<0.001), continuity (β=1.27, P<0.001), coordination(referral) (β=-1.42, P<0.001), comprehensiveness(utilization) (β=-1.70, P<0.001), comprehensiveness(provision) (β=-0.99, P<0.001), family-centeredness(β=-0.52, P<0.01), community orientation(β=-1.78, P<0.001), than those who did not receive contract services after controlling socio-demographic and service utilization factors. There were no statistically significant differences in the dimensions of coordination (information system) (β=-0.25, P=0.137) and culture orientation (β=-0.264, P=0.056), than those who did not receive family doctor contract services. Conclusions This study demonstrates that the pilot implementation of family doctor contract services has significantly improved patients’ perceived primary care quality in the pilot cities, and could help solve the quality problem of primary care. It needs further promotion across the province.
Canine SARS-CoV-2 infection
Authors: Sit Thomas HC , Brackman Christopher J , Ip Sin Ming , Tam Karina WS , Law Pierra YT , To Esther MW , Yu Veronica YT , Sims Leslie D , Tsang Dominic NC , Chu Daniel KW , Perera Ranawaka APM , Poon Leo LM , Peiris Malik 
Published: March 2020

Abstract
SARS-CoV-2 emerged in Wuhan in December 2019 and has caused the pandemic respiratory disease, COVID-19. Following what is presumed to be an initial zoonotic transmission event, the virus is now spreading efficient in humans. Very little is known about the susceptibility of domestic mammals kept as pets to this virus. Samples were collected over a 13-day period from a 17 year-old neutered male Pomeranian in Hong Kong SA that was taken into isolation after two members of the household tested positive for the virus. Nasal swabs were consistently positive on the five occasions the dog was tested using quantitative RT- PCR with viral loads between 7.5xE2 to 2.6 x10E4 RNA copies per mL of sample. The dog remained asymptomatic. Cultures attempted on three RT-PCR positive nasal samples were negative. Gene sequences from samples from two household members were identical. The viral sequence from the dog differed at three nucleotide positions; two of these resulted in amino acid changes but their significance is yet to be determined. Seroconversion was not detected but this was expected given the asymptomatic infection and low virus load. The evidence suggests that this is an instance of human-to-animal transmission of SARS-COV-2. It is likely that we could see similar events in other infected households. We do not have information yet on whether this virus can cause illness in dogs but no specific signs were seen in this dog. Whether infected dogs could transmit the virus to other animals or back to humans remains unknown. In this case it did not appear to have occurred.
Aloperine Induces Apoptosis by a Reactive Oxygen Species Activation Mechanism in Human Ovarian Cancer Cells
Authors: Qiu Mingning , Liu Jie , Su Yongxia , Liu Jianjun , Wu Chenchen , Zhao Baoyu 
Published: March 2020

Background:
Ovarian cancer is the most lethal gynecologic malignancy worldwide with
poor prognosis owing to chemotherapy resistance and cancer relapse. Hence, there is an urgent
need to develop novel anticancer agents against ovarian cancer.

Objective:
The aim of this research is to investigate the possible anticancer activity of aloperine, an
active ingredient from a traditional Chinese medicine Sophora alopecuroides, and to explore the
possible Reactive Oxygen Species (ROS)-related mechanism.

Methods:
Cell viability, cytotoxicity, apoptosis, ROS generation, and oxidant stress indicators
were analyzed.

Results:
Our results demonstrated that aloperine significantly induced inhibition of cell viability,
promoted cytotoxicity and mitochondrial-related apoptosis, and increased ROS generation in
ovarian cancer cells. Furthermore, the antioxidant α-lipoic acid reversed apoptosis in aloperinetreated
cells. In addition, we identified hydrogen peroxide as the main type of ROS, and the antioxidant
catalase suppressed the apoptotic inducing effect of aloperine whereas hydrogen peroxide supplement
exacerbated the effect of aloperine in ovarian cancer cells.

Conclusion:
Taken together, our results indicated that aloperine could exert anti-ovarian cancer
cell activity through a reactive oxygen species activation mechanism and suggested aloperine as a
potential agent against ovarian cancer.
Fractional Exhaled Nitric Oxide and Nanomaterial Exposure in Workplaces
Authors: Iavicoli Ivo , Fontana Luca , Leso Veruscka , Macrini Maria Carmela , Pelclova Daniela 
Published: March 2020

Background:
The widespread application of engineered nanomaterials (ENMs) and the
increasing likelihood of general and occupational exposure raised concerns on their possible human
health impact. ENMs, in fact, may induce alterations in different organ systems, and particularly in
the respiratory tract. This makes it important to identify possible biomarkers of early lung effect in
exposed workers. In this regard, the possibility to use the fractional exhaled levels of nitric oxide
(FENO) in biological monitoring has attracted considerable interest.

Objective:
To comprehensively assess the role of FENO as a possible biomarker of lung effect in
ENM exposed workers.

Methods:
A systematic search was performed on Pubmed, Scopus, and ISI Web of Knowledge
databases according to the PRISMA guidelines.

Results:
Seven studies investigated FENO in workers exposed to different kinds of metal-(i.e.
silver and gold), metal oxide- (titanium and silica dioxide), and carbon-based ENMs (carbon nanotubes).
In general, no significant alterations were detected between exposed workers and controls.

Conclusions:
Definite conclusion on the function of FENO in occupational biological monitoring
cannot be extrapolated due to the limited number of available studies and the small size of investigated
populations. Additionally, the lack of environmental monitoring data and the fragmented
knowledge on ENM modes of action prevent to establish dose-response relationships. Future research
appears necessary to deeply define the possibility to employ FENO as an early biomarker of
lung effects taking in consideration possible occupational exposure issues, i.e. differently characterized
ENMs and work tasks, as well as individual influencing factors, i.e. smoking and atopy.
Magnolia officinalis Reduces Inflammation and Damage Induced by Recurrent Status Epilepticus in Immature Rats
Authors: Vega-García Angélica , Rocha Luisa , Guevara-Guzmán Rosalinda , Guerra-Araiza Christian , Feria-Romero Iris , Gallardo Juan M. , Neri-Gomez Teresa , Suárez-Santiago José E. , Orozco-Suarez Sandra 
Published: March 2020

Background:
Neuroinflammation induced in response to damage caused by status epilepticus (SE)
activates the interleukin (IL)1-β pathway and proinflammatory proteins that increase vulnerability to the development
of spontaneous seizure activity and/or epilepsy.

Objectives:
The study aimed to assess the short-term anti-inflammatory and neuroprotective effects of Magnolia
officinalis (MO) on recurrent SE in immature rats.

Methods:
Sprague-Dawley rats at PN day 10 were used; n = 60 rats were divided into two control groups, SHAM
and KA, and two experimental groups, MO (KA-MO) and Celecoxib (KA-Clbx). The anti-inflammatory effect of
a single dose of MO was evaluated at 6 and 24 hr by Western blotting and on day 30 PN via a subchronic administration
of MO to assess neuronal preservation and hippocampal gliosis by immunohistochemistry for NeunN and
GFAP, respectively.

Results:
KA-MO caused a decrease in the expression of IL1-β and Cox-2 at 6 and 24 h post-treatment, a reduction
in iNOS synthase at 6 and 24 hr post-treatment and reduced neuronal loss and gliosis at postnatal day 30,
similar to Clbx.

Conclusion:
The results indicating that Magnolia officinalis is an alternative preventive treatment for early stages
of epileptogenesis are encouraging.