bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transglutaminase and tyrosinase as potential cross‐linking tools for the improvement of rheological properties of gluten‐free amadumbe dough
Authors: Manhivi Vimbainashe E. , Amonsou Eric O. , Kudanga Tukayi 
Published: December 2019

SummaryGluten‐free bread remains of poor quality despite efforts to amend gluten‐free flours with ingredients such as hydrocolloids and proteins. Enzymatic modification of the proteins in dough may result in polymers that mimic gluten. This research investigated the effects of transglutaminase and tyrosinase on the rheological properties of amadumbe dough. Tyrosinase oxidation resulted in a 7.7–39.4% decrease in dough‐free amine, and a 16.8–46.3% decrease in the dough thiol content as activity was increased from 0 to 80 U g−1 flour. Transglutaminase treatment decreased the dough‐free amino groups by 10–38.1% as activity was increased from 0 to 2 U g−1 flour. Evidence of tyrosinase and transglutaminase‐mediated cross‐linking was provided by relevant model reactions monitored by mass spectrometry. An increase in dough G′ and G″ showed that both transglutaminase and tyrosinase improved dough viscoelasticity. The increase in the viscoelasticity of the dough potentially improves carbon dioxide retention during proofing.
Psychometric properties of the Arabic version of the International Consultation on Incontinence Questionnaire on Long‐Term Catheter Quality of Life
Authors: Youssef Naglaa , Best Catherine , Mackay William Gordon , Hagen Suzanne , Shepherd Ashley 
Published: December 2019

AbstractAimTo translate the International Consultation on Incontinence Questionnaire (ICIQ) Long‐Term Catheter Quality of Life (LTCqol) questionnaire (ICIQ‐LTCqol) from English to Arabic and evaluate its psychometric properties.BackgroundCurrently, no tool for Arabic‐speaking patients is available to measure the quality of life in patients using long‐term urinary catheters.DesignTranslation and psychometric assessment of questionnaire.MethodsThe internal consistency and construct validity of the translated Arabic version of the ICIQ‐LTCqol were evaluated in a convenience sample of 141 participants recruited from a hospital in Egypt from April to September 2017. Test‐retest reliability was assessed for a sample of 15 participants who completed this version at two time points. Construct validity was assessed by factor analysis.ResultsThe translated Arabic version of the ICIQ‐LTCqol showed satisfactory test‐retest reliability and internal consistency, with the Cronbach α = .75. Confirmatory factor analysis confirmed the same two factors (“catheter function” and “lifestyle”) structure as found in the English version of the tool supporting the construct validity of the translated questionnaire.ConclusionThis original and significant study allows, for the first time, researchers and clinicians working with Arabic‐speaking patients, the opportunity to evaluate the quality of life in long‐term urinary catheter users.
Pricing and introductory scarcity strategies based on consumers' response
Authors: Chen Shaohua , Jia Junxiu 
Published: December 2019

AbstractIn industries such as electronics, automobiles, and luxury goods, we have observed numerous cases of intentional undersupply when a new product is introduced. Previous research has shown that introductory scarcity has a positive effect on consumers' willingness to pay, but this effect has yet to be quantified. This paper investigates how scarcity strategies affect product pricing and a firm's profits. We consider two types of scarcity strategies, a real and a fake scarcity strategy, and propose a two‐period model in both markdown and markup scenarios. We find that an introductory scarcity strategy is profitable in a markup scenario only if the discount factor is greater than a critical value and payoffs are compartmentalized. Moreover, an introductory scarcity strategy could result in profits in a markdown scenario if consumers are sensitive to scarcity. However, a scarcity strategy is always detrimental to a firm with low‐quality products in both scenarios. If there are uninformed consumers in the market, a fake scarcity strategy is beneficial for firms in the short term but not in the long term.
Ocular thermography in ocular inflammation and dry eye
Authors: Acosta M. Carmen 
Published: December 2019

AbstractMeasurement of the ocular surface temperature (OST) has been proposed as a diagnostic method for ocular surface pathologies as dry eye disease (DED), due to the involvement in its pathophysiology of tear evaporation rate.During mild inflammation induced by UV radiation in guinea pigs, no changes in the initial OST measured immediately after eye opening and in the rate of OST decrease during the interblink interval (IBI) were observed. However, during allergic keratoconjunctivitis, where tearing and blinking were significantly increased and a prominent inflammation was induced, the initial OST was significantly increased.In animals with experimentally‐induced tear deficiency, a variability in the initial OST values was found: some animals showed a “cold spot” (increasing in intensity and size during IBI) in the central cornea, while in other animals initial OST was warmer than control, suggesting a subclinical inflammation.Eyes from Sjögren syndrome patients, besides a reduced tearing rate, showed higher initial OST and faster ocular surface cooling between blinks than eyes from age‐matched healthy subjects.Although OST values per se do not provide relevant information on ocular surface disease, due to the differences in etiology and pathophysiology of DED, it may be a valid diagnostic method if combined with the clinical evaluation of inflammation, and tear volume, composition and stability.Support: AEI‐ERDF SAF‐2017‐83674‐C2‐1‐R and 2‐R; Generalitat Valenciana PROMETEO/2018/114.
iSyTE: A web resource tool to expedite eye gene discovery
Authors: Lachke Salil 
Published: December 2019

AbstractThe identification of genes linked to eye development and its associated defects presents a formidable challenge. In the recent past however, genome‐level transcript profiling (transcriptomics) has become increasingly feasible due to technologies such as microarrays and RNA sequencing. Their application to interrogate specific eye tissues and cell types holds high promise to impact ocular gene discovery. However, global gene expression profiling has brought with it new challenges, such as parsing through the large amounts of data to prioritize select candidates for further analysis. Toward this goal, the development of strategies such as “in silico subtraction” has led to the construction of a systems‐based web‐resource tool called iSyTE (integrated Systems Tool for Eye gene discovery). In this presentation, I will discuss the impact of iSyTE and eye‐tissue transcriptomics on identification of new genes linked to lens biology and cataract, and outline the ongoing efforts for extending this approach to other ocular tissues such as the retina and the cornea.
History of the development of intraocular lenses
Authors: Eppig Timo 
Published: December 2019

AbstractCouching as the first surgical method for cataract has been performed for about 4000 years. Until mid of the 20th century, attention was spent on removing the opaque lens from the line of sight – refractive correction was provided as spectacle lenses. During World War II – the British ophthalmologist Harold Ridley had to treat pilots suffering from penetrating eye injuries after being shot down. He found that splints from the cockpit cover – which were made from PMMA – did not induce inflammatory reactions. Inspired by his student Steve Parry he started to develop an intraocular implant made of PMMA – which was the birth of the modern intraocular lens. The invention of phacoemulsification by Charles Kelman and the capulorhexis by Neuhann and Gimbel led the way to the success of modern posterior chamber lenses. Until the late 1970’s, lenses were made of PMMA until the first flexible silicone lenses came up. Starting in the late 1980s the silicone lenses were successively replaced by acrylic lenses. Nowadays the majority of lenses are made from acrylic material.
Optical concepts – monofocal, aspheric, toric, multifocal, EDOF
Authors: Kaymak Hakan 
Published: December 2019

AbstractWe will present an overview of the various optical concepts used in intraocular lenses: while the conventional monofocal lens was designed to provide basic refractive correction, modern aspheric lenses aim to provide better contrast sensitivity by compensation of intrinsic or corneal spherical aberration. Toric lenses were developed to correct corneal astigmatism during cataract surgery. Multifocal lenses aim to provide functional visual acuity at various distance levels. There are basically two concepts of implementing multifocality: those based on refraction and those based on diffraction. Modern extended depth of focus lenses try to fill in the gap between monofocal and multifocal lenses by providing acceptable visual acuity from far distance to intermediate distance.
Matching analysis with your study design
Authors: Kivelä Tero 
Published: December 2019

AbstractThis talk will highlight some key issues in choosing statistical methods for ophthalmic research, based on personal experience of the speaker as a writer, reviewer and editorial board member. The items discussed, based on examples from recent literature, will include: (1) proper ways of summarising and comparing visual acuities; (2) proper ways of analysing data collected from both eyes of the same patients, (3) proper ways of analysing longitudinal follow‐data, and (4) proper ways of analysing contigency table data. Correct reporting and analysis of data related to the eye is not too difficult as long as one is aware of a few basic rules. Putting them into action helps to ensure that the conclusions you put forward and those that the readers draw from your study will be proper.
Comparison of conjunctival vascularity changes using optical coherence tomography angiography after trabeculectomy and phacotrabeculectomy
Authors: Hyun Seo Je 
Published: December 2019

AbstractPurposeTo compare the bleb vascularity using optical coherence tomography angiography (OCT‐A) between mitomycin‐C (MMC) augmented trabeculectomy and phacotrabeculectomy and to investigate the relationship between bleb vascularity and surgical outcomes.MethodsWe retrospectively reviewed data for 72 eyes from 72 glaucoma patients who underwent MMC augmented trabeculectomy with/without cataract surgery. Bleb area scans were obtained using OCT‐A during the preoperative period and 1, 2, 4, and 6 weeks, and 2, 4, and 6 months postoperatively. For conjunctival vascularity analysis, a semi‐automated program was used to calculate the densities of color and brightness of the selected area.ResultsColor and brightness densities were decreased in the trabeculectomy group during all periods, whereas it did not decrease in the phacotrabeculectomy group at 4 and 6 weeks, 2, 4, and 6 months. The color and brightness densities were significantly higher in the phacotrabeculectomy group than in the trabeculectomy group after 6 weeks and 2, 4, and 6 months. A Kaplan‐Meier survival graph showed that the intraocular pressure was not different according to surgery type, but glaucoma type. Logistic regression analysis revealed that the brightness density 1 week postoperation was correlated with reoperation.ConclusionsFollowing trabeculectomy with MMC, The brightness density one week postoperation may be a predictive index for surgical outcomes, suggesting that a more accurate prediction could be made if evaluation of bleb structural analysis and bleb vascularity are performed simultaneously.
Tips and tricks on how to write a good scientific paper
Authors: Oddone Francesco 
Published: December 2019

AbstractWriting for peer‐reviewed publication is an important part of the careers of many clinicians and researchers. Writing a paper and getting it published is hard work, even after the hard work that led to the publishable results. The presentation will focus on tips and tricks about how to make this process easier through an organized step by step algorithm to guide the writer from the first paragraph to the last in a clear and effective way.
How to get your research known by your peers!
Authors: Barbosa Breda Joao 
Published: December 2019

AbstractNowadays it is almost as important to properly divulge your research as to actually make it. A paper’s real impact is certainly linked to the amount of people that read it, cite it and, in some cases, put it to use in the clinics/labs. The whole purpose of research is to lead to changes according to the new evidence, and that is only possible if the message is passed on to the audience/readers. This talk will focus on how to create a clean and concise message that can be used in the channels that allow your research results to be disseminated. An overview of the different platforms that can be used to get your research known will also be performed. The goal is to enable Young Ophthalmologists and/or Researchers with the tools to more effectively transport the message imbedded in the research work they have done to their peers.
Understanding organelle biogenesis during the autophagy process in the RPE
Authors: Sinha Debasish 
Published: December 2019

AbstractThe retinal pigmented epithelium (RPE) is critically important to retinal homeostasis, in part due to it's very active processes of phagocytosis and autophagy. Both of these processes depend upon the normal functioning of lysosomes, organelles which must fuse with (auto)phagosomes to deliver the hydrolases that effect degradation of cargo. It has become clear that signaling through mTOR complex 1 (mTORC1), is very important in the regulation of lysosomal function. Lysosomes are cellular organelles that modulate various processes such as autophagy and heterophagy, plasma membrane repair, cholesterol homeostasis, and cell death. The number, size, and content of lysosomes vary in different cell types. The distribution of lysosomes within the cell is determined by the nutrient sensing machinery at the lysosomal membrane, and is an important factor in lysosomal catabolic function. In this course, we will focus on the effector molecules present in RPE cells that impact the lysosomal‐autophagic pathway in retinal health and disease.
Efficacy of an ophthalmic fixed combination of tetracycline, chloramphenicol and colistin on planktonic growth and preformed biofilm
Authors: Rita Blanco Anna , Nostro Antonia , D’ Arrigo Manuela , Marino Andreana 
Published: December 2019

AbstractPurposeBacteria colonization on the lid margin could play a crucial role in developing of biofilm causes of chronic infections such as blepharo‐conjunctivitis or lacrimal system disease. The aim of this study was to evaluate the efficacy of an ophthalmic fixed antibiotics/steroid combination (AC) containing tetracycline (TET), chloramphenicol (CAF) and colistin (CS), in the ratio 2:1:3, on bacterial planktonic growth and preformed biofilm.MethodsThe antimicrobial effect of AC against Staphylococcus aureus 815 and Pseudomonas aeruginosa 1 ocular strains was evaluated by time‐killing curves analysis at 30’, 2, 4, 6, 8 and 24 hr. The activity of AC (exposure of 2 and 6 hr) on preformed 24 hr‐biofilm was evaluated by cell viability and biomass measurements, expressed as logarithm of colony‐forming units/ml (Log10 CFU/ml) and optical density values, respectively.ResultsTime‐killing curves showed that AC was able to reduce the load of planktonic growth of both strains versus control. A significant reduction of 5 Log10 CFU/ml after 8h for S. aureus and after 4h for P. aeruginosa was reported. The AC was active against preformed biofilm, with a viability significant reduction (>2 Log10 CFU/ml versus control) of both strains at the exposure times. A biomass reduction of 40% and 60% for S. aureus and P. aeruginosa respectively, was detected at the exposure time of 2 hr and maintained at 6 hr.ConclusionsThe three antibiotics CAF, TET and CS present in AC showed a significant antimicrobial and antibiofilm effect. Antibiotics combination is a promising strategy for increasing treatment efficacy and for controlling resistance evolution. The results of this study, taken together, suggest the applications of this fixed combination in the treatment of chronic ocular infections where planktonic cells and biofilm are involved.Keywordsantibiotics combination, biofilm, ocular infection, lacrimal system
Pattern‐stimulation in the complex management of patients with operated retinal detachment
Authors: Kazanskaya Tamara 
Published: December 2019

AbstractIntroductionThe problem of increasing visual function after surgical treatment of retinal detachment retains its relevance. Existing methods do not take into account the possibilities of the retinal‐cortex system stimulation.ObjectiveEvaluation of the functional effect of pattern‐stimulation in the complex management of retinal detachment operated patients.Patients and methodsForty patients with an extrascleral retinal procedure. The median of age is 53.6 [23; 75] years. Patients in group 1a (10 eyes with macula‐on before surgery) and 1b group (with macula‐off before surgery) underwent a course of drug therapy (peptides, meldonium, antioxidants), patients of group 2a (10 eyes macula‐on before surgery) and 2b Groups (macula‐off before surgery) – a similar course of drug treatment combined with a pattern‐stimulation course. All groups are comparable in age, sex, visual acuity, data of electrophysiological studies – macular electroretinogram (ERG), visual evoked cortical potentials (VECP). According to the results of optical coherent tomography carried out before the start of treatment, there were no gross organic changes in the area of the macula in patients. Starting treatment 6 months after surgery. Evaluation of the treatment effectiveness after 3 months after its completion.ResultsIncreased visual acuity after treatment in group Ia occurred at 0.05, in group Ib by 0.03, in group IIa by 0.17, in group IIb by 0.21. The amplitude of wave‐a of macular ERG increased by 4.07 mV in group Ia, by 5.4 mV in group Ib, by 2.9 mV in group IIa, by 8.17 mV in group IIb; changes in wave amplitude‐in different directions: in group Ia, an increase of 3.5 mV, in group Ib a decrease of 0.4 mV, in group IIa and IIb an increase of 5.19 mV and 10.86 mV, respectively. VECP N 75 latency decreased by group Ia by 1.46 ms, by group 19.2 ms by group IIa, by 14.94 ms by group IIb, and increased by 1.1 ms by group Ib; P100 decrease in groups: Ia by 0.93 ms, IIa by 14.39 ms, IIb by 14.94 ms, increase in group Ib by 1.2 ms; the amplitude of the N75‐P100 VECP wave increased in all groups: Ia by 1.3 mV, Ib by 1.55 mV, IIa by 3.48 mV, IIb by 10.58 mV. An increase in visual acuity on the background of drug treatment correlated with an improvement in the amplitude characteristics of the local ERG (r = 0.47), using pattern stimulation with an increase in the amplitude characteristics of the VECP (r = 0.46).ConclusionUsing the stimulation pattern in complex management of the retinal detachment operated patients helps to improve the amplitude indices of local ERG and VECP by more than three times, reducing VECP latency values, increasing visual acuity 4 times in comparison with the routine drug therapy results in patients 1a and 1b groups. The results obtained indicate the feasibility of using the method of pattern‐stimulation in patients with operated retinal detachment to enhance the functional effect of surgical treatment.
Difference of corneal endothelial cells according to senescence
Authors: Joo Shin Young , Sun Hwang Jin , Kyung Jang Yoon 
Published: December 2019

AbstractPurposeBullous keratopathy is a painful corneal endothelial disease and its treatment requires corneal transplantation because human corneal endothelial cells (HCECs) do not proliferate in vivo. In this study, we evaluated differences between HCECs depending on senescence.MethodsHCECs were cultured and were classified into two groups: cells with small size and mosaic pattern (normal group), and cells with large size and no mosaic pattern (SA group). Cell shape, cell viability, and cell proliferation rate were evaluated. Cell cycle analysis, mitochondrial oxidative stress, and mitochondrial membrane potential were assessed. Western blotting was performed to evaluate expression of various proteins.ResultsCell shape and size were different between normal and SA groups. Cell viability and cell proliferation rates were found to be lower in the SA group. Cell cycle analysis, mitochondrial oxidative stress, and mitochondrial membrane potential revealed differences between normal and SA groups. Protein expression was different between the normal group and the SA group.ConclusionsDifferences in phenotype between normal and SA groups might contribute to the pathogenesis of corneal endothelial diseases.References

Siu GD, Young AL and Jhanji V. Alternatives to corneal transplantation for the management of bullous keratopathy. Curr Opin Ophthalmol. 2014; 25(4):347–352.

Choi SO, Jeon HS, Hyon JY, Oh YJ, Wee WR, Chung TY, Shin YJ and Kim JW. Recovery of Corneal Endothelial Cells from Periphery after Injury. PLoS One. 2015; 10(9):e0138076.

Eghrari AO, Riazuddin SA and Gottsch JD. Overview of the Cornea: Structure, Function, and Development. Prog Mol Biol Transl Sci. 2015; 134:7–23.
Development of a nomogram using fundus photography to predict glaucoma progression in patients showing disc hemorrhage
Authors: Kim Sangah , Won Bae Hyoung 
Published: December 2019

AbstractPurposeTo develop a nomogram to predict the progression of glaucoma by fundus photography in patients with disc hemorrhage.Material and methodsRetrospective review of the medical records of patients with disc hemorrhage, which was detected during follow up with open angle glaucoma, from January 2010 to January 2019. Patients were divided into glaucoma progression (n = 52) or non‐progression (n = 38) groups. We assessed proximal location and morphology of disc hemorrhage; relationship to retinal nerve fiber layer defects with disc hemorrhage; and angular extent of disc hemorrhage, between groups using fundus photography. Multiple logistic regression analysis was performed to select prognostic factors, and we constructed a nomogram to predict glaucoma progression.ResultThe incidences of disc hemorrhage at the border of retinal nerve fiber layer defects (p < 0.001) and peripapillary disc hemorrhage (p = 0.009) were significantly higher in the progression group. We used angular extent; location of disc hemorrhage with retinal nerve fiber layer defects; and proximal location of disc hemorrhage to construct the nomogram. The area under the receiver operating characteristic curve of the nomogram was 0.829.ConclusionWe created the nomogram using fundus photography in patients showing disc hemorrhage as a novel and accurate screening method to predict glaucoma progression and aid clinicians to decide on the best treatment plan.