bluuebunny/crossref_metadata_2025_split huggingface dataset:
Awareness among French healthcare workers of the transmission of multidrug resistant organisms: a large cross-sectional survey
Authors:   the PerceptR Study group, Vaillant L. , Birgand G. , Esposito-Farese M. , Astagneau P. , Pulcini C. , Robert J. , Zahar J. R. , Sales-Wuillemin E. , Tubach F. , Lucet J. C. 
Published: November 2019

Abstract

Background
Much effort has been made over the last two decades to educate and train healthcare professionals working on antimicrobial resistance in French hospitals. However, little has been done in France to assess perceptions, attitudes and knowledge regarding multidrug resistant organisms (MDROs) and, more globally, these have never been evaluated in a large-scale population of medical and non-medical healthcare workers (HCWs). Our aim was to explore awareness among HCWs by evaluating their knowledge of MDROs and the associated control measures, by comparing perceptions between professional categories and by studying the impact of training and health beliefs.

Methods
A multicentre cross-sectional study was conducted in 58 randomly selected French healthcare facilities with questionnaires including professional and demographic characteristics, and knowledge and perception of MDRO transmission and control. A knowledge score was calculated and used in a logistic regression analysis to identify factors associated with higher knowledge of MDROs, and the association between knowledge and perception.

Results
Between June 2014 and March 2016, 8716/11,753 (participation rate, 74%) questionnaires were completed. The mean knowledge score was 4.7/8 (SD: 1.3) and 3.6/8 (SD: 1.4) in medical and non-medical HCWs, respectively. Five variables were positively associated with higher knowledge: working in a university hospital (adjusted odds ratio, 1.41, 95% CI 1.16–1.70); age classes 26–35 years (1.43, 1.23–1.6) and 36–45 years (1.19, 1.01–1.40); medical professional status (3.7, 3.09–4.44), working in an intensive care unit (1.28, 1.06–1.55), and having been trained on control of antimicrobial resistance (1.31, 1.16–1.48). After adjustment for these variables, greater knowledge was significantly associated with four cognitive factors: perceived susceptibility, attitude toward hand hygiene, self-efficacy, and motivation.

Conclusions
We found a low level of MDRO awareness and knowledge of associated control measures among French HCWs. Training on hand hygiene and measures to control MDRO spread may be helpful in shaping beliefs and perceptions on MDRO control among other possible associated factors. Messages should be tailored to professional status and their perception. Other approaches should be designed, with more effective methods of training and cognitive interventions.

Trial registration
Clinical Trials.gov NCT02265471. Registered 16 October 2014 - Retrospectively registered.
From quality to outcomes: a national study of afterschool STEM programming
Authors: Allen Patricia J. , Chang Rong , Gorrall Britt K. , Waggenspack Luke , Fukuda Eriko , Little Todd D. , Noam Gil G. 
Published: November 2019

AbstractBackgroundState afterschool networks across the US are engaged in system-building efforts to improve the quality of science, technology, engineering, and math (STEM)-focused afterschool programming. This study examined national trends in STEM program quality, youth outcomes, and the connections between these two data sources.MethodsOne thousand five hundred ninety-nine youths (grades 4–12) enrolled in 158 STEM-focused afterschool programs across 11 state networks completed a retrospective self-assessment measuring STEM attitudes and social-emotional learning (SEL)/twenty-first-century skills. Two hundred fifty standardized observations of STEM activities were performed to measure STEM program quality.Results(1) Most youth (65–85%) reported increases in STEM engagement, identity, career interest, career knowledge, relationships, critical thinking, and perseverance, with the largest gains reported by those engaging with STEM activities for 4 weeks or more; (2) there were significant, strong correlations between STEM and SEL/twenty-first-century outcomes reported by youth; and (3) youth participating in higher-quality STEM programming reported more growth than peers participating in lower-quality programs.ConclusionThis effort demonstrates how investments in STEM program quality yield high returns for programs and youth and how collaborations between research and practice can track successes and challenges, determine investments in program management, and expand advocacy and policy efforts. Additionally, this study supports a growing body of literature that suggests a synergy between youth development and STEM learning approaches that can improve outcomes for youth.
The 32nd summer school of the Research Community for Mechanisms of Mutations
Authors: Kawanishi Masanobu 
Published: November 2019

Abstract
The 32nd summer school of the Research Community for Mechanisms of Mutations was held at Inter-University Seminar House in Hachioji city, Tokyo, from September 7 to 8, 2019. Thirty-eight people attended this annual event, and three eminent researchers were invited to discuss DNA damage induced by endogenous aldehydes, “action-at-a-distance mutagenesis” and a novel genome editing method, and DNA repair in fungi and plants. In addition to these plenary sessions, eleven participants presented their own research in oral sessions. More than half of the participants were young scientists such as graduate/undergraduate students, post-doctoral fellows and assistant professors. All members joined in enthusiastic discussions and acquired new scientific knowledge through these two days.
Expert consensus on relevant risk predictors for the occurrence of osteoporotic fractures in specific clinical subgroups – Delphi survey
Authors: Bodmer Nicolas S. , Häuselmann Hans Jörg , Frey Diana , Aeberli Daniel , Bachmann Lucas M. 
Published: November 2019

Abstract

Background
There is an ongoing discussion about incorporating additional risk factors to established WHO fracture risk assessment tool (FRAX) to improve the prediction accuracy in clinical subgroups. We aimed to reach an expert consensus on possible additional predictive parameters for specific clinical subgroups.

Methods
Two-round modified Delphi survey: We generated a shortlist of experts from the authors’ lists of the pertinent literature and complemented the list with experts known to the authors. Participants were asked to name possible relevant risk factors besides the FRAX-parameters for the occurrence of osteoporotic fractures. Experts specified these possible predictors for specific subgroups of patients. In the second round the expert panel was asked to weight each parameter of every subgroup assigning a number between one (not important) to ten (very important). We defined the threshold for an expert consensus if the interquartile range (IQR) of a predictor was ≤2. The cut-off value of the median attributed weights for a relevant predictor was set at ≥7.

Results
Eleven experts of seven countries completed both rounds of the Delphi. The participants agreed on nine additional parameters for seven categories. For the category “secondary osteoporosis”, “older adults” and “nursing home patients”, there was a consensus that history of previous falls was relevant, while for men and postmenopausal women, there was a consensus that the spine fracture status was important. For the group “primary and secondary osteoporosis” the experts agreed on the parameters “high risk of falls”, “lumbar spine bone mineral density (BMD)” and “sarcopenia”.

Conclusion
This Delphi survey reached a consensus on various parameters that could be used to refine the currently existing FRAX for specific clinical situations or patient groups. The results may be useful for studies aiming at improving the predictive properties of instruments for fracture prediction.
Can lung ultrasound assist in the decision of weaning mechanically ventilated neonates?
Authors: Eltomey Mohamed Adel , Shehata Ahmed Hany , Nassar Mohamed Abd Ellatiff , Elmashad Abd Elrahman Mohamed 
Published: November 2019

Abstract

Background
Mechanical ventilation is widely used in neonates presenting with respiratory distress; however, it is not without complications. Early weaning of neonates is vital however the process of extubation is difficult to determine precisely. The use of lung ultrasound in neonates is recently accepted as a reliable tool in the assessment of several lung conditions. The aim of this study was to assess the use of lung ultrasound score as a quantitative method to assist in the decision of weaning mechanically ventilated neonates.

Results
The study was conducted on 40 neonates admitted to the NICU with different causes of respiratory distress needing respiratory support by mechanical ventilation. Lung ultrasound was performed using a linear superficial probe at least three times, at admission, before switching mechanical ventilation mode and before weaning. The initial lung ultrasound score for all patients was between 9 and 36 (mean 25 ± 6.97, median 26) with the 11 patients initiated on Sync. Intermittent ventilation (SIMV) showing significantly lower scores than those initiated on assist/control ventilation (ACV). Patients successfully switched from ACV to SIMV showed significantly lower scores than those who failed. Patients successfully weaned from SIMV showed significantly lower scores than those who failed. ROC analysis showed a cut-off score of ≤ 14 had 85% sensitivity and 100% specificity for a successful switch between ACV to SIMV. It also showed that a score ≤ 6 had 87.5% sensitivity and 100% specificity for successful extubation.

Conclusion
The use of quantitative lung ultrasound scores in assessment of mechanically ventilated neonates shows a great potential in aiding the process of weaning.
Different aspects in manipulating overlapped spectra used for the analysis of trimebutine maleate and structure elucidation of its degradation products
Authors: Lotfy Hayam M. , Morgan Eman M. , Fayez Yasmin Mohammed , Abdelkawy M. 
Published: November 2019

AbstractBackgroundFour rapid, accurate, and validated stability-indicating spectrophotometric methods have been described in the present work for the analysis of trimebutine maleate (TM) in existence of its degradation products in its authentic form and in pharmaceutical formulations excluding any separation steps.ResultsThese methods were a dual-wavelength (DW) method which allows the determination of TM in existence of its degradation products at 243 nm and 269 nm, second derivative (D2) method measured at peak amplitude at 268 nm, ratio difference (RD) method at 242 nm and 278 nm, and constant center coupled with spectrum subtraction (CC-SS) method at 242 nm and 278 nm versus 278 nm. By applying the suggested methods, TM could be quantified in the range of 5.0–60.0 μg/mL with percentage recoveries 99.97 ± 0.40, 100.36 ± 0.58, 99.90 ± 0.42, and 100.15 ± 0.45 for DW,D2, RD, and CC-SS methods, respectively. International Conference on Harmonization guidelines were followed for validation of the described methods, and the application of laboratory-prepared mixtures along with different pharmaceutical drugs including the target drug showed favorable results without any contribution from additives.ConclusionsStatistical comparison was used to compare the proposed and official methods, and satisfactory results for both accuracy and precision were obtained. The results confirm the applicability of the suggested methods for the determination of TM in quality control laboratories.
Long-Term Mental Health Outcomes in Mothers and Siblings of Children With Cancer: A Population-Based, Matched Cohort Study
Authors: van Warmerdam Jacqui , Sutradhar Rinku , Kurdyak Paul , Lau Cindy , Pole Jason D. , Nathan Paul C. , Gupta Sumit 
Published: November 2019

PURPOSE Although a diagnosis of childhood cancer can have a profound effect on the entire family unit, its impact on the long-term mental health of family members is not well characterized. METHODS A provincial childhood cancer registry in Ontario, Canada, was linked to birth records to identify separate population-based cohorts of mothers and siblings of children diagnosed with cancer between 1998 and 2014. The mother and sibling cohorts were matched to corresponding population controls and linked to health services data. The rate of mental health–related outpatient visits (family physician, psychiatrist) and the incidence of severe psychiatric events (psychiatric emergency department visit, psychiatric hospitalization, suicide) were compared between mothers and siblings and their controls. Possible predictors of mental health outcomes were examined, including demographics, characteristics of the cancer-affected child, and cancer treatment. RESULTS We identified 4,773 mothers and 7,897 siblings of children diagnosed with cancer during the study period. Compared with controls, both groups experienced elevated rates of outpatient visits (mothers: rate ratio [RR], 1.4; P < .0001; siblings: RR, 1.1; P < .0001). The risk of severe psychiatric events was not increased in either cohort. Mother and sibling demographic factors associated with increased risk of adverse mental health included younger maternal age at cancer diagnosis, low socioeconomic status, and rural residence among mothers and older sibling age among siblings. Treatment-related variables pertaining to the cancer-affected child were not associated with mental health outcomes. Mental health outcomes clustered within families. CONCLUSION Both mothers and siblings experience elevated and prolonged need for mental health–related health care as compared with the general population. Demographic risk factors predict subpopulations at highest risk. Increased psychosocial support for family members during and after cancer therapy is warranted.
Harmonization of Tumor Mutational Burden Quantification and Association With Response to Immune Checkpoint Blockade in Non–Small-Cell Lung Cancer
Authors: Vokes Natalie I. , Liu David , Ricciuti Biagio , Jimenez-Aguilar Elizabeth , Rizvi Hira , Dietlein Felix , He Meng Xiao , Margolis Claire A. , Elmarakeby Haitham A. , Girshman Jeffrey , Adeni Anika , Sanchez-Vega Francisco , Schultz Nikolaus , Dahlberg Suzanne , Zehir Ahmet , Jänne Pasi A. , Nishino Mizuki , Umeton Renato , Sholl Lynette M. , Van Allen Eliezer M. , Hellmann Matthew D. , Awad Mark M. 
Published: November 2019

PURPOSE Heterogeneity in tumor mutational burden (TMB) quantification across sequencing platforms limits the application and further study of this potential biomarker of response to immune checkpoint inhibitors (ICIs). We hypothesized that harmonization of TMB across platforms would enable integration of distinct clinical data sets to better characterize the association between TMB and ICI response. METHODS Cohorts of patients with non–small-cell lung cancer sequenced by 1 of 3 targeted panels or by whole-exome sequencing (WES) were compared (N = 7,297). TMB was calculated uniformly and compared across cohorts. TMB distributions were harmonized by applying a normal transformation followed by standardization to z scores. In subcohorts of patients treated with ICIs (Dana-Farber Cancer Institute n = 272; Memorial Sloan Kettering Cancer Center n = 227), the association between TMB and outcome was assessed. Durable clinical benefit (DCB) was defined as responsive/stable disease lasting ≥ 6 months. RESULTS TMB values were higher in the panel cohorts than in the WES cohort. Average mutation rates per gene were highly concordant across cohorts (Pearson’s correlation coefficients, 0.842-0.866). Subsetting the WES cohort by gene panels only partially reproduced the observed differences in TMB. Standardization of TMB into z scores harmonized TMB distributions and enabled integration of the ICI-treated subcohorts. Simulations indicated that cohorts > 900 are necessary for this approach. TMB did not associate with response in never-smokers or patients who harbor targetable driver alterations, although these analyses were underpowered. An increase of TMB thresholds increased DCB rate, but DCB rates within deciles varied. Receiver operating characteristic curves yielded an area under the curve of 0.614, with no natural inflection point. CONCLUSION The z score conversion harmonizes TMB values and enables integration of data sets derived from different sequencing panels. Clinical and biologic features may provide context to the clinical application of TMB and warrant additional study.
In Situ Observation of Efflorescence and Deliquescence Phase Transitions of Single NaCl and NaNO3 Mixture Particles in Air Using a Laser Trapping Technique
Authors: Ishizaka Shoji , Guo Fangqin , Tian Xiaomeng , Seng Samantha , Tobon Yeny A , Sobanska Sophie 
Published: November 2019

Abstract
A novel experimental approach to study the hygroscopic properties of multi-component inorganic aerosols was demonstrated using a laser trapping technique. The efflorescence and deliquescence phase transitions of the equimolar mixture of NaCl and NaNO3 particles levitated in air were reversibly induced by controlling relative humidity. The two-stage phase transitions of the particles during the dehumidifying and humidifying processes were successfully observed in air. To our knowledge, this is the first experimental result to observe the reversible hygroscopic behavior of single optically-levitated multi-component inorganic aerosols in air. Furthermore, to elucidate the influence of solid substrates on the homogeneous and heterogeneous nucleation processes, the efflorescence relative humidity (ERH) and mutual efflorescence relative humidity (MERH) in air were compared with those observed on a hydrophobic glass substrate. The average ERH and MERH values of the NaCl–NaNO3 particles levitated in air were lower than those obtained for the particles deposited on the hydrophobic glass substrate.
Variable expression and silencing of CRISPR-Cas9 targeted transgenes identifies the AAVS1 locus as not an entirely safe harbour
Authors: Bhagwan Jamie R. , Collins Emma , Mosqueira Diogo , Bakar Mine , Johnson Benjamin B. , Thompson Alexander , Smith James G.W. , Denning Chris 
Published: November 2019

Background: Diseases such as hypertrophic cardiomyopathy (HCM) can lead to severe outcomes including sudden death. The generation of human induced pluripotent stem cell (hiPSC) reporter lines can be useful for disease modelling and drug screening by providing physiologically relevant in vitro models of disease. The AAVS1 locus is cited as a safe harbour that is permissive for stable transgene expression, and hence is favoured for creating gene targeted reporter lines. Methods: We generated hiPSC reporters using a plasmid-based CRISPR/Cas9 nickase strategy. The first intron of PPP1R12C, the AAVS1 locus, was targeted with constructs expressing a genetically encoded calcium indicator (R-GECO1.0) or HOXA9-T2A-mScarlet reporter under the control of a pCAG or inducible pTRE promoter, respectively. Transgene expression was compared between clones before, during and/or after directed differentiation to mesodermal lineages. Results: Successful targeting to AAVS1 was confirmed by PCR and sequencing. Of 24 hiPSC clones targeted with pCAG-R-GECO1.0, only 20 expressed the transgene and in these, the percentage of positive cells ranged from 0% to 99.5%. Differentiation of a subset of clones produced cardiomyocytes, wherein the percentage of cells positive for R-GECO1.0 ranged from 2.1% to 93.1%. In the highest expressing R-GECO1.0 clones, transgene silencing occurred during cardiomyocyte differentiation causing a decrease in expression from 98.93% to 1.3%. In HOXA9-T2A-mScarlet hiPSC reporter lines directed towards mesoderm lineages, doxycycline induced a peak in transgene expression after two days but this reduced by up to ten-thousand-fold over the next 8-10 days. Nevertheless, for R-GECO1.0 lines differentiated into cardiomyocytes, transgene expression was rescued by continuous puromycin drug selection, which allowed the Ca2+ responses associated with HCM to be investigated in vitro using single cell analysis. Conclusions: Targeted knock-ins to AAVS1 can be used to create reporter lines but variability between clones and transgene silencing requires careful attention by researchers seeking robust reporter gene expression.
Equitable data sharing: challenges and suggestions for ways forward
Authors: Barnes Karen I. , Canario Julio Arturo , Vernekar Sunil S. , Goudar Shivaprasad S. , Espinal Roberto , Merson Laura , Cheah Phaik Yeong 
Published: November 2019

Data sharing is increasingly mandated by health research funders and publishers. In the context of data collected in low-resource settings, concerns have been raised regarding inequitable opportunities to engage in secondary use of data between researchers in well-resourced and resource-limited settings. In this context, we present three case studies and their issues related to equity: the multicountry Antenatal Corticosteroid Trial, health research in the Dominican Repulic and the WorldWide Antimalarial Resistance Network. These case studies were discussed at the 2018 Global Forum for Bioethics in Research in South Africa, focussing on the theme “The ethics of data sharing and biobanking in health research”. The case studies provide concrete examples of real challenges such as lack of prior consent for data sharing, potential for misinterpretation of data by secondary users and limited capacity of researchers in low-resource settings to conduct secondary analyses. We conclude by suggesting ways forward. We stress the importance of capacity building and investments in data management and data science skills, and in data sharing platforms supporting poverty-related disease research. In addition, we recommend that there should be incentives to promote data sharing and that research groups and institutions establish their own data sharing policies tailored to their context, data and community while persuing harmonization with existing policies as much as possible. We also think that international guidelines on authorship criteria should be revisited. For new studies, researchers should obtain consent for sharing of participants’ data with secondary users. Lastly we recommend that community and stakeholder engagement be conducted to improve the consent process and identify what might be sensitive data to mitigate any potential harms to data subjects and their communities.
Using simulated patients as a learning strategy to support undergraduate nurses to develop patient-teaching skills
Authors: Coleman Deborah , McLaughlin Dorry 
Published: November 2019

Background: An increase in the number of patients with long-term conditions has required a greater focus on nurse-led educational interventions to enable patients to develop self-management strategies. However, patient education is frequently taken for granted, and nurses sometimes consider that their undergraduate training does not prepare them to participate in effective patient teaching. Aim: The study aimed to formatively evaluate a simulated role-play scenario facilitated with third-year nursing students to support the development of patient-teaching skills. Method: The study combined two approaches to simulation, using high-fidelity and mid-fidelity simulation scenarios sequentially. This enabled students (n=20) to apply the communication strategies learnt to both a skills-based procedural situation and a patient-teaching simulation. A five-item pro forma with four open questions and one closed question was used for formative evaluation. Findings: The results indicated that using a simulated patient to practise patient-teaching skills was perceived by the students to be a valuable method of learning that they could transfer to clinical practice. Conclusion: The findings suggested that facilitating learning with a simulated patient is useful in replicating authentic verbal and practical interactions with a patient in practice.