bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multiomics global landscape of stemness-related gene clusters in adipose-derived mesenchymal stem cells
Authors: Lu Guan-Ming , Rong Yong-Xian , Liang Zhi-Jie , Hunag Dong-Lin , Ma Yan-Fei , Luo Zhi-Zhai , Wu Fang-Xiao , Liu Xin-Heng , Liu Yu , Mo Steven , Qi Zhong-Quan , Li Hong-Mian 
Published: July 2020

Abstract
Background
Adipose-derived mesenchymal stem cells (AD-MSCs) are a type of stem cell that is abundant and widely used. The molecular characteristics of AD-MSCs from different passages from donors of different ages have not been well elucidated.

Methods
Six kinds of AD-MSCs ((E1, E2, E3, Y1, Y2, and Y3) with E denoting cells derived from an elderly patient, Y denoting cells derived from a young patient, and 1, 2, and 3 representing passages 3, 6, and 10) were obtained from human abdominal adipose tissue. We obtained the protein expression profile, the mRNA expression profile, the lncRNA expression profile, and the methylation profile of each kind of AD-MSC by sequencing. After calculating the stemness indices, genes related to stemness were extracted. The multiomics correlation analysis was performed in the stemness-related genes. In addition, short time-series expression miner (STEM) analysis was performed for all cell passages and donor ages. To further explore the biological functions of the stemness-related genes, we performed Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses. Finally, the lncRNA-KEGG network and transcription factor (TF)-KEGG network were constructed based on the RNAInter database and TRRUST v2 database.

Results
The stemness of the Y1, E1, and Y2 cells was higher than that of the E2, Y3, and E3 cells. The stemness was the highest for Y1 cells and the lowest for E3 cells. STEM analysis showed that five stemness-related gene clusters were associated with the cell passages, and only one gene cluster was associated with age. The enrichment analysis results showed that the biological processes (BPs) and KEGG pathways were mainly involved in the proliferation, differentiation, and migration of cells. The global regulatory landscape of AD-MSCs was constructed: 25 TFs and 16 lncRNAs regulated 21 KEGG pathways through 27 mRNAs. Furthermore, we obtained a core stemness-related gene set consisting of ITGAV, MAD2L1, and PCNA. These genes were expressed at higher levels in Y1 cells than in E3 cells.

Conclusion
The multiomics global landscape of stemness-related gene clusters was determined for AD-MSCs, which may be helpful for selecting AD-MSCs with increased stemness.
Study on track planning problem of multi-constrained and double-targeted
Authors: Nie Yi , Xie Xiangjun , Ding Xianfeng , Zhang Qianyu , Zhou Jingyu 
Published: July 2020

AbstractAiming at the positioning error of the aircraft, the error accumulation to a certain extent may lead to the failure of the mission. A track correction method based on oriented graph search was proposed, and the dynamic programming idea was applied to solve the path optimization problem. Firstly, the correction points in the flight space were preprocessed, and the planning of the correction points in the flight space was transformed into a graph theory problem. It effectively solves the problem that traditional methods did not adapt well to dynamic changes in flight space. And the problem of too high complexity in the calculation space. Using the dynamic programming idea to carry out the flight path planning for the flight space, the path-correction number optimal double-objective model is comprehensively established. Finally, the oriented graph and dynamic programming ideas were used to solve the problem, and the visual analysis of the aircraft track was realized. The simulation results show that the established path-corrected optimal double-objective model can calculate an optimal track with relatively small correction times and short length.
Comparison of [18F]FDG PET/CT with magnetic resonance imaging for the assessment of human brown adipose tissue activity
Authors: Fischer Jonas Gabriel William , Maushart Claudia Irene , Becker Anton S. , Müller Julian , Madoerin Philipp , Chirindel Alin , Wild Damian , ter Voert Edwin E. G. W. , Bieri Oliver , Burger Irene , Betz Matthias Johannes 
Published: July 2020

AbstractBackgroundBrown adipose tissue (BAT) is a thermogenic tissue which can generate heat in response to mild cold exposure. As it constitutes a promising target in the fight against obesity, we need reliable techniques to quantify its activity in response to therapeutic interventions. The current standard for the quantification of BAT activity is [18F]FDG PET/CT. Various sequences in magnetic resonance imaging (MRI), including those measuring its relative fat content (fat fraction), have been proposed and evaluated in small proof-of-principle studies, showing diverging results. Here, we systematically compare the predictive value of adipose tissue fat fraction measured by MRI to the results of [18F]FDG PET/CT.MethodsWe analyzed the diagnostic reliability of MRI measured fat fraction (FF) for the estimation of human BAT activity in two cohorts of healthy volunteers participating in two prospective clinical trials (NCT03189511, NCT03269747). In both cohorts, BAT activity was stimulated by mild cold exposure. In cohort 1, we performed [18F]FDG PET/MRI; in cohort 2, we used [18F]FDG PET/CT followed by MRI. Fat fraction was determined by 2-point Dixon and 6-point Dixon measurement, respectively. Fat fraction values were compared to SUVmeanin the corresponding tissue depot by simple linear regression.ResultsIn total, 33 male participants with a mean age of 23.9 years and a mean BMI of 22.8 kg/m2were recruited. In 32 participants, active BAT was visible. On an intra-individual level, FF was significantly lower in high-SUV areas compared to low-SUV areas (cohort 1:p < 0.0001 and cohort 2:p = 0.0002). The FF of the supraclavicular adipose tissue depot was inversely related to its metabolic activity (SUVmean) in both cohorts (cohort 1:R2 = 0.18,p = 0.09 and cohort 2:R2 = 0.42,p = 0.009).ConclusionMRI FF explains only about 40% of the variation in BAT glucose uptake. Thus, it can currently not be used to substitute [18F] FDG PET-based imaging for quantification of BAT activity.Trial registrationClinicalTrials.gov.NCT03189511, registered on June 17, 2017, actual study start date was on May 31, 2017, retrospectively registered.NCT03269747, registered on September 01, 2017.
Automated synthesis, preclinical toxicity, and radiation dosimetry of [18F]MC225 for clinical use: a tracer for measuring P-glycoprotein function at the blood-brain barrier
Authors: Toyohara Jun , Sakata Muneyuki , Tago Tetsuro , Colabufo Nicola A. , Luurtsema Gert 
Published: July 2020

Abstract
Introduction
[18F]MC225 is a selective substrate for P-glycoprotein (P-gp) that has good metabolic stability and shows higher baseline uptake compared with other P-gp substrates such as (R)-[11C]Verapamil. Prior to clinical translation, it is necessary to perform process validation of the radiosynthesis, assessment of preclinical toxicity, and radiation dosimetry.

Methods
The production of [18F]MC225 was automated on a CFN-MPS200 multipurpose synthesizer. The acute toxicity of MC225 was evaluated at a dose of 2.5 mg/kg bodyweight, which is more than 10,000-fold the postulated maximum clinical dose of [18F]MC225. The acute toxicity of [18F]MC225 injection at a 200-fold dose, to administer a postulated dose of 185 MBq of [18F]MC225, was also evaluated after the decay-out of 18F. The mutagenicity of MC225 was studied by a reverse mutation test using Salmonella typhimurium and Escherichia coli (Ames test). In vivo biodistribution and dosimetry studies of [18F]MC225 were carried out in normal mice. Human dosimetry was estimated using OLINDA software.

Results
The mean decay-corrected yields of [18F]MC225 at end of synthesis were 13%, with > 99% radiochemical purity, > 1000 GBq/μmol molar activity, and ≤ 1.5 μg/185 MBq of total chemical contents. All process validation batches complied with the product specifications and the process was confirmed to be appropriate for the production of [18F]MC225. No acute toxicity of MC225 or [18F]MC225 injection was found. No mutagenic activity was observed for MC225. The biodistribution study demonstrated both hepatobiliary and renal excretion of radioactivity. The most critical organ was the pancreas, with (63.8 μGy/MBq) or without urination (63.9 μGy/MBq) at 360 min after injection. The estimated effective dose (μSv/MBq) with and without urination at 360 min after injection was calculated as 15.7 and 16.9, respectively.

Conclusion
[18F]MC225 shows acceptable pharmacological safety at the dose required for adequate PET imaging. The potential risk associated with [18F]MC225 PET imaging is well within acceptable dose limits.
Effects of beta-conglycinin intake on circulating FGF21 levels and brown adipose tissue activity in Japanese young men: a single intake study and a randomized controlled trial
Authors: Taniguchi Hirokazu , Shimizu Keigo , Wada Sayori , Nirengi Shinsuke , Kataoka Haruki , Higashi Akane 
Published: July 2020

Abstract
Background
Human brown adipose tissue (BAT) activity has beneficial effects on body composition and glucose metabolism. A previous study reported that beta-conglycinin intake induced postprandial fibroblast growth factor 21 (FGF21) secretion, thereby promoting adipose tissue thermogenesis in mice. Since it has not been evaluated whether beta-conglycinin intake is associated with induced FGF21 secretion and BAT thermogenesis in humans, the current study examined the effects of beta-conglycinin intake on circulating FGF21 level and BAT activity.

Methods
Twenty-two healthy young male subjects participated. This study consisted of 2 interventional studies. In one of them, the effects of single beta-conglycinin intake at thermoneutral temperature on circulating FGF21 levels were examined (n = 7). The other study was a single-blinded randomized crossover trial of 2 weeks (n = 14). The subjects were exposed to mild cold conditions using a climatic chamber, and BAT activity was analyzed using thermography. Serum FGF21 level was determined by ELISA in these studies.

Results
In the single intake study, serum FGF21 level was the highest before beta-conglycinin intake and gradually and significantly decreased throughout the 2-h experimental period (P < 0.05). The randomized crossover trial showed that 2-week beta-conglycinin intake did not affect serum FGF21 level and BAT activity, whereas changes (Δ) in baseline levels of serum FGF21 were positively correlated with Δ BAT activity (P < 0.05). In addition, analysis of each group revealed that there was significant correlation between the Δ serum FGF21 level and Δ BAT activity in the beta-conglycinin group (P < 0.05), but not in the placebo group.

Conclusions
This study reveals that although serum FGF21 levels are not increased by a single or short-term intake of beta-conglycinin, the Δ basal FGF21 level is associated with Δ BAT activity. These results suggest that human FGF21 responsiveness is different from that of rodents and support the importance of FGF21 in human BAT thermogenesis.

Trial registration
This study is registered with University Hospital Medical Information Network in Japan (number 000038723, https://upload.umin.ac.jp/cgi-open-bin/ctr_e/ctr_view.cgi?recptno=R000043942).
Mental health issues impacting pharmacists during COVID-19
Authors: Elbeddini Ali , Wen Cindy Xin , Tayefehchamani Yasamin , To Anthony 
Published: July 2020

AbstractThe coronavirus disease 2019 (COVID-19) impact on the mental health of healthcare workers is extremely detrimental. It is imperative that the psychological health of all healthcare workers be protected. However, an often overlooked member of the healthcare frontline is the pharmacist. Pharmacists provide many types of essential services during the pandemic, which often cannot be done from a remote location. Being frontline healthcare workers, pharmacists have experienced an increase in the number of patients seen, the amount of screening and triage being done, the amount of COVID-19 information being delivered, the number of medication shortages, and the amount of workplace harassment taking place. These activities increase the amount of stress, burden, and frustration felt by pharmacists have a negative impact on their mental health and well-being. This article seeks to address the specific implications of COVID-19 on the mental health of pharmacists.
Antimicrobials administration time in patients with suspected sepsis: faster is better for severe patients
Authors: Jouffroy Romain , Vivien Benoît 
Published: July 2020

AbstractIn a recent report, Ascuntar et al. describes the impossibility to demonstrate a significant association between early antibiotic therapy administration and mortality in sepsis patients. Nevertheless, we believe that some methodological issues deserve their conclusions. First, the objective of the authors of an early antibiotic therapy may be ambitious considering practical daily emergency department limitation. Second, most of patients included in the study appear to suffer from sepsis and not from septic shock, which limits the impact of an early and aggressive management. At last, more than a single intervention such as antibiotic therapy, sepsis treatment is now considered as based on a “bundle of care.”
Kinetics and statistical analysis of the bio-stimulating effects of goat litter in crude oil biodegradation process
Authors: Ani Kingsley Amechi , Chukwuma Emmanuel Chibundo 
Published: July 2020

Abstract
Background
The kinetics and statistical analysis of crude oil (CO) degradation in CO-contaminated soil (COCS) using goat litter (GL) were investigated. The data obtained from the CO degradation process was fitted to the first- and second-order kinetic models. The effects of process parameters such as temperature and the initial CO concentrations on the CO degradation process were also investigated. The one-way ANOVA and Turkey’s post-hoc analysis were also used to study the statistical significance of the process parameters on the CO degradation process.

Results
The microbial count showed that the GL contained a total viable count (TVC), coliform, and mold counts of 2.6 × 107 CFUg−1, 2.6 × 107 CFUg−1, and 6.9 × 103 CFUg-1, respectively. The error and linear regression analysis between experimental and model-predicted values revealed that the first-order kinetic model gave a better explanation of the CO degradation process. The rejection of the null hypothesis was evident from one-way ANOVA and Turkey’s post-hoc analysis as the P values at a temperature of 30 °C and initial CO concentrations of 70 gL−1 and 90 gL−1 were less than the significant level of 0.05. Notable organic nutrients in the GL which were beneficial in the COCS treatment process as indicated by the Fourier-transform infrared spectrophotometer (FTIR) analysis were phosphorous and nitrogen.

Conclusions
It may be concluded that GL could be used as an effective organic treatment for COCS at CO initial concentrations of 70 and 90 gL-1 and a temperature of 30 °C.
Evaluating the impact of nurse practitioner involvement in a pleural procedures clinic
Authors: Peter Eleanor 
Published: July 2020

Background: Pleural disease incidence is increasing and there has been a move towards outpatient management. Pleural clinics have been developed for patients to be assessed and have pleural procedures for diagnostic and symptomatic purposes. Aim: The study aimed to assess the impact of nurse practitioner (NP) involvement in a pleural procedures clinic on clinic capacity, and staff and patient experience. Methods: A mixed-methods service evaluation was completed in a medium-sized general hospital. The number of patients seen in clinic over a 6-month period at the beginning of NP involvement was compared with the number of patients seen 1 year later. Semi-structured interviews of key staff stakeholders were conducted. Findings: Clinic capacity increased from 4.57 to 5.86 patients per list. NP involvement increased access, improved patient care, reduced work burden for other staff and improved educational opportunities. Conclusion: The impact of NP involvement in the pleural clinic was seen as positive and increased clinic capacity. The same model of practice could be considered elsewhere.
An international survey of pediatric and neonatal clinicians' vascular access practice: PediSIG assessment of vascular access, education, and support (PAVES) catheter selection
Authors: Davis Mary Beth Hovda , Takashima Mari , Girgenti Constance , Ullman Amanda J. 
Published: July 2020

Highlights There is a wide variance in neonatal and pediatric vascular access workforce models, training, and competency assessments. Pain control during procedures is critical for children, yet it is not consistently used. Procedural support has shown improved patient outcomes, yet is not standardly used for every distressful procedure. Core standards are needed to ensure proper training and support for the pediatric and neonatal vascular access clinicians. Background: Despite evidence to support best practice in neonatal and pediatric venipuncture delivery and procedural support, there are inconsistencies in practice. To inform future research, education, and workforce innovation, the Association for Vascular Access Pediatric Special Interest Group (PediSIG) developed and undertook a survey to describe the current vascular access practice for clinicians caring for neonatal and pediatric patients. Objective: Describe the current state of workforce models, training, and clinical practices surrounding pediatric and neonatal vascular access. Design: Cross-sectional, electronic survey using convenience sampling. Settings: International clinicians who provide vascular access (peripheral intravenous catheter insertion, venipuncture for blood sampling) for neonatal and pediatric patients. Methods: An electronic survey was developed by the PediSIG. The survey covered workforce models, clinician training and competency, pain relief, procedural support, and device securement. The electronic survey was then distributed to the PediSIG membership and shared among several neonatal/pediatric email lists. Data were analyzed descriptively, with an exploration of association between clinical outcomes, workforce, and training. Results There were 242 responses from 5 countries showing a wide variance of practice. Workforce models showed many different team names and responsibilities along with a variance of personnel and staffing hours. Clinician training was described as 4 hours or less by 44% (n = 69) of respondents. Less than half of the responses (47%; n = 99) reported having a formal procedure to escalate a patient to an expert care and not having a set number of max attempts before escalation. Only two-thirds (n = 115) of respondents said they had a standardized protocol for pain control and procedural support, with only 13% (n = 23) and 15% (n = 27), respectively, self-reporting that they always followed the protocol. Conclusions The respondents reported a wide variance in neonatal and pediatric vascular access procedures and the resources used to support this practice. Core standards need to be developed to help guide neonatal and pediatric clinicians and their institutions. The standards should encompass recommendations for workforce models, proper training, competency, insertion guidelines, pain control,
Towards the integration of nanoemitters by direct laser writing on optical glass waveguides
Authors: Xu Xiaolun , Broussier Aurélie , Ritacco Tiziana , Nahra Mackrine , Geoffray Fabien , Issa Ali , Jradi Safi , Bachelot Renaud , Couteau Christophe , Blaize Sylvain 
Published: July 2020

A major challenge towards nanophotonics is the integration of nanoemitters on optical chips. Combining the optical properties of nanoemitters with the benefits of integration and scalability of integrated optics is still a major issue to overcome. In this work, we demonstrate the integration of nanoemitters positioned in a controlled manner onto a substrate and onto an optical ion-exchanged glass waveguide via direct laser writing based on two-photon polymerization. Our nanoemitters are colloidal CdSe/ZnS quantum dots (QDs) embedded in polymeric nanostructures. By varying the laser parameters during the patterning process, we make size-controlled QD-polymer nanostructures that were systematically characterized using optical and structural methods. Structures as small as 17 nm in height were fabricated. The well-controlled QD-polymer nanostructure systems were then successfully integrated onto a new photonic platform for nanophotonics made of an ion-exchanged waveguide. We show that our QDs maintain their light emitting quality after integration as verified by photoluminescence (PL) measurements. Ultimately, QD emission coupled to our waveguides is detected through a home-built fiber-edge coupling PL measurement setup. Our results show the potential for future integration of nanoemitters onto complex photonic chips.
Conceptions of Truth in Plato’s

Sophist
Authors: Peramatzis Michail 
Published: July 2020

Abstract
The paper seeks to specify how, according to Plato’s Sophist, true statements achieve their being about objects and their saying that ‘what is about such objects is’. Drawing on the 6th definition of the sophist, I argue for a normative-teleological conception of truth in which the best condition of our soul –in its making statements or having mental states– consists in its seeking to attain the telos of truth. Further, on the basis of Plato’s discussion of original and image, his distinction between correct and incorrect image, and the 7th definition, I argue that achieving the telos of truth involves preserving the original’s proportions and appropriate features. The view that Plato’s conception of truth takes statements or mental states to be certain types of image is not ground-breaking. The important contribution of my argument is that it offers a plausible way to understand two recalcitrant claims made by Plato: first, that falsity obtains not only in the region of incorrect images (appearances) but also within correct images (likenesses); second, that some incorrect images are based on knowledge and so could be true.
Kant über die Vollständigkeit der „Tafel der Kategorien der Freiheit“
Authors: Zimmermann Stephan 
Published: July 2020

Abstract
Kant’s repeated statement in the Critique of Pure Reason that the so-called table of judgements and, as a consequence, the table of pure concepts of the understanding or categories, is fully exhaustive is well-known. This ambitious assertion has worried and challenged generations of authors. However, thus far the entire discussion has completely disregarded the fact that in his Critique of Practical Reason Kant undertakes a coordinate venture. For the “Table of the Categories of Freedom”, which he sets out, with only a few explanations, in the “Second Chapter” of the Critique, Kant makes, when looked at more closely, the very same claim to completeness. This article shows that this same claim is made, even though Kant does not state this assertion as explicitly as one might have expected. And it poses the question, which underlying, yet concealed assumptions this view might appeal to. Or is it that Kant has merely failed to provide the evidence in this case?
A unified representation for some interpolation formulas
Authors: Masjed-Jamei Mohammad , Moalemi Zahra , Koepf Wolfram 
Published: July 2020

Abstract
As an extension of Lagrange interpolation, we introduce a class of interpolation formulas and study their existence and uniqueness.
In the sequel, we consider some particular cases and construct the corresponding weighted quadrature rules.
Numerical examples are finally given and compared.