bluuebunny/crossref_metadata_2025_split huggingface dataset:
Immunomodulatory effects of pepsin-educed soy protein hydrolysate in rats and murine cells
Authors: Ashaolu Tolulope Joshua , Yantiam Niracha , Yupanqui Chutha Takahashi 
Published: December 2019

Background: There has been an increase in demand for soy and its products, with soy protein having a huge market in particular. Several reports have established how soy protein hydrolysates (SPHs) yield better physiological properties and play essential functional roles than the crude soy protein.Objective: To investigate the immunomodulatory and acute toxicity of soy protein hydrolysate (SPH) produced with pepsin protease in mice and rats.Methods: Soy protein hydrolysate was enzymatically produced using pepsin, with E/S (enzyme to substrate ratio) of 0.5% (250 u/mg) and hydrolysis time of 4h. Afterwards, the SPH effects on murine spleen lymphocyte proliferation, and peritoneal macrophage phagocytosis were investigated in vitro. Confirmation studies were explored in rats’ sera IgG and IgA, and the acute toxic effect of SPH was observed in mice subjects.Results: The hydrolysate increased the levels of splenocytes (stimulative index of 1.269 - 1.402) and peritoneal macrophages (phagocytic index of 0.717 – 1.304). Furthermore, the concentrations of sera IgG and IgA obtained from SPH-fed rats ranged from 0.198 – 0.345 mg/ml and 0.0184 – 0.0194 mg/ml, respectively in comparison with the soy protein isolate (SPI) -fed rats (0.208 – 0.322 mg/ml and 0.0188 – 0.0189 mg/ml, respectively). Additionally, 10 mg dose of SPH stably elicited serum IgG in contrast to other doses, while there was a general decrease in the amounts of IgA obtained in the rat subjects. Moreover, there was no acute toxic effect recorded in the mice subjects. Conclusion: In light of the results, it is possible that SPH prepared with pepsin has the potential of improving the immune system, and may therefore be used as immunomodulatory or functional food product.Keywords: Soy protein hydrolysates, splenocyte proliferation, immunomodulatory, phagocytosis and pepsin
A novel protodioscin-enriched fenugreek seed extract (Trigonella foenum-graecum, family Fabaceae) improves free testosterone level and sperm profile in healthy volunteers
Authors: Bagchi Debasis , Swaroop Anand , Maheshwari Anuj , Verma Narsingh , Tiwari Kiran , Bagchi Manashi , Preuss Harry G. , Kumar Pawan 
Published: December 2019

Background: A novel, patent-pending 20% protodioscin-enriched extract has been developed in our laboratories from fenugreek seeds (Furosap®). We assessed the efficacy of Furosap® in 50 male volunteers (age: 35-65 years) on free and total increased testosterone levels, sperm profile, mental alertness, cardiovascular health, mood, libido, and quality of life.Methods: Furosap® (500 mg/day/subject) was administered to the 50 male volunteers over a period of 12 weeks in a one-arm, open-labelled study, to determine the efficacy on free and total testosterone levels, sperm profile and sperm morphology, libido and erectile dysfunction, mood and mental alertness and broad spectrum safety parameters. Institution Review Board approval was obtained for this study and the study was registered at the clinicaltrials.gov (NCT02702882). Results: A statistically significant increase in free testosterone levels were observed in these volunteers following supplementation of Furosap®. Sperm morphology, sperm counts, mental alertness, mood, cardiovascular health, and libido performance were significantly improved. Extensive blood chemistry analyses revealed broad spectrum safety. No significant changes were observed in serum lipid function, cholesterol, triglyceride, HDL and LDL levels, and hemogram. Conclusions: Results confirmed that this protodioscin-enriched extract from fenugreek seeds (Furosap) is safe and efficacious in boosting serum free testosterone levels, healthy sperm profile, mental alertness, cardiovascular health, and overall performance in male volunteers.Keywords: Standardized fenugreek seed extract; Protodioscin; Testosterone booster; Sperm profile; Mood alleviation; Safety
Glucosinolates and isothiocyanates from broccoli seed extract suppress protein glycation and carbonylation
Authors: Shimoda Hiroshi , Hirano Marina , Takeda Shogo , Hitoe Shoketsu 
Published: December 2019

Background: Glucosinolates from brassica plants are hydrolyzed by internal or salivary myrosinase to produce isothiocyanates. Glucoraphanin, a major glucosinolate in broccoli, is hydrolyzed to sulforaphane (SFN), which exhibits antitumor and detoxification activities. Regarding the influence of broccoli and its constituents on the skin, a few studies have reported anti-inflammatory and antioxidant effects. Recently, advanced glycation end products (AGEs) and carbonyl proteins have been reported to accelerate skin aging. Objective: We evaluated the effects of broccoli seed extract (BSE) and glucosinolates on protein glycation and carbonylation in vitro.Methods: To evaluate the effects of BSE and its constituents, protein glycation and carbonylation were induced by mixing fructose with bovine serum albumin (BSA) and then measuring production of AGEs, fructosamine, and carbonyl proteins (CP). Production of CP after mixing fatty acids with BSA was also assessed. Furthermore, the effect of BSE and its constituents on CP production by human fibroblasts (TIG103) was examined.Results: BSE suppressed the production of AGEs, fructosamine, and CP after mixing fructose and BSA. BSE also suppressed production of CP when oxidized linoleic acid was mixed with BSA. Isothiocyanates, including SFN and iberin, suppressed fructose-based CP production, but SFN had no effect on CP production stimulated by oxidized linoleic acid. In contrast, glucosinolates from BSE did not suppress fructose-based CP production, but suppressed CP production due to oxidized linoleic acid. Among the glucosinolates in BSE, glucoberteroin showed the strongest suppression of CP production. CP production in fibroblasts was also suppressed by glucosinolates, including glucoiberin and glucoberteroin.Conclusions: BSE demonstrated anti-glycation and anti-carbonylation effects on protein reactions with fructose and oxidized fatty acids. Isothiocyanates suppressed protein carbonylation induced by fructose, but not those induced by oxidized lipids. On the other hand, glucosinolates (precursors of isothiocyanates) did not suppress protein carbonylation induced by fructose, but suppressed carbonylation due to oxidized lipids. Moreover, glucoberteroin and glucoiberin suppressed CP production in a cell-based assay. These results suggest that glucosinolates in BSE could suppress protein carbonylation leading to aging of the skin. Keywords: Broccoli; glucosinolate; sulforaphane; carbonylation; glycation; glucoberteroin; glucoiberin
Late introduction of low dose resveratrol and grape powder after estradiol depletion does not restore glucose tolerance in the ovariectomized rat.
Authors: Anderson Eoin , Cervone Dan , Dyck David James 
Published: December 2019

Background: Estrogen (E2) loss is associated with insulin resistance. Natural compounds such as resveratrol (RESV) have potential insulin sensitizing effects. Grape pomace powder (GP) also contains RESV and other antioxidants. However, the ability of realistic, attainable concentrations of RESV and GP to reverse glucose intolerance in E2 deficient rats has not yet been explored.Purpose: The aim of the current study was to determine whether RESV and GP, in realistic amounts that could be achieved with supplementation, would be effective in restoring glucose tolerance in the ovariectomized (OVX) rat. Furthermore, there appears to be a critical time window following the loss of E2 when hormonal replacement is effective, with delayed treatment being ineffective and potentially detrimental. Therefore, we were particularly interested in examining the effectiveness of RESV and GP as a delayed treatment i.e. after the establishment of glucose intolerance, rather than administering at the onset of E2 loss. Results: In the present study, rats demonstrated impaired glucose tolerance, as determined by an intraperitoneal glucose tolerance test, 12 weeks after bilateral ovary removal. Subsequently, OVX animals were randomly placed into a sham or one of 3 treatment groups. The treatments were either i) a physiological oral dose of E2 (28µg/kg body mass), ii) RESV (5mg/kg body mass), or iii) GP (1.5g/100g of diet) for another 6 weeks. OVX animals were significantly heavier than non-OVX rats at the onset of glucose intolerance and this did not change throughout the treatment. None of the treatments restored glucose tolerance within the 6 weeks. Insulin tolerance did not worsen in OVX rats and was unaffected by treatment. Adipocyte size was generally increased in OVX animals and was not decreased with treatment.Conclusions: In conclusion, delayed E2, RESV and GP treatment do not restore glucose tolerance in OVX rats. Low dose RESV and GP supplementation may not be effective alternatives to HRT to restore compromised glucose tolerance. Keywords: ovariectomy, estrogen, resveratrol, grape pomace, glucose tolerance, insulin tolerance, delayed treatment
Metformin inhibits expression of the proinflammatory biomarker inducible nitric oxide synthase in hepatocytes
Authors: Nakatake Richi , Iida Hiroya , Ishizaki Morihiko , Matsui Kosuke , Nakamura Yusuke , Kaibori Masaki , Nishizawa Mikio , Okumura Tadayoshi 
Published: December 2019

Background: Metformin is used to treat patients with type II diabetes. However, there are few scientific reports on its anti-inflammatory effects. In the inflamed liver, proinflammatory cytokines stimulate liver cells, followed by inducible nitric oxide synthase (iNOS) expression. Excessive NO levels produced by iNOS have been implicated as a factor in liver injury. As a result, it is essential to inhibit iNOS induction to prevent liver injury.Objective: This study aimed to investigate liver protective effects of metformin by examining interleukin (IL)-1β-stimulated hepatocytes. Methods: Primary cultured rat hepatocytes were treated with interleukin (IL)-1β in the presence or absence of metformin. iNOS induction and its signaling pathway were analyzed.Results: Metformin decreased iNOS protein and mRNA expression, resulting in the inhibition of hepatic NO production. Metformin also reduced tumor necrosis factor (TNF)-α and IL-6 mRNA expression. Metformin inhibited an essential signaling pathway for iNOS induction, type I IL-1 receptor upregulation. Transfection experiments revealed that metformin reduced iNOS mRNA levels through both promoter transactivation and mRNA stabilization. Delayed metformin administration after IL-1β addition also inhibited iNOS induction. Conclusions: Metformin affects the induction of inflammatory mediators including iNOS and TNF-α, demonstrating its therapeutic potential for organ injuries, including the liver.Keywords: metformin, inducible nitric oxide synthase, liver injury, primary cultured hepatocytes, type I interleukin-1 receptor, tumor necrosis factor-α
Facial Paralysis in Longitudinal versus Oblique and Otic-Sparing versus Non Otic-Sparing Temporal Bone Fractures
Authors: Chua Ruben , Lacanilao Rene 
Published: December 2019

Objective: To compare the proportion of temporal bone fractures using traditional (longitudinal vs. transverse) and otic involvement (otic sparing vs. non-otic sparing) classification schemes and their relationship with the development of facial paralysis.
Methods:
Design: Retrospective Case Series
Settings: Tertiary Government Hospital
Patients: Records of 49 patients diagnosed with temporal bone fracture in our institution from August 2016 to June 2018.
Results: A total of 41 records of patients with temporal bone fractures, 32 males, 9 females, aged 5 to 70 years-old (mean 37.5-years-old) were included. In terms of laterality 23 (56%) involved the right and 17 (41%) the left side. Traditionally classified, 32 (78%) were longitudinal and 9 (22%) were transverse. Using newer classification based on otic involvement and non-otic involvement, 38 (93%) were otic-sparing and 3 (7%) were non otic-sparing. Only 9 (22%) out of 41 total fracture patients developed facial paralysis, involving 7 of the 32 longitudinal fractures and 2 of the 9 transverse fractures, or 8 of the 38 otic-sparing and 1 out of 3 non otic-sparing fractures.
Conclusion: Because of the small sample size, no conclusions regarding the proportion of temporal bone fractures using traditional (longitudinal vs. transverse) and otic involvement (otic sparing vs. non-otic sparing) classification schemes and their relationship with the development of facial paralysis can be drawn in this study.
Keywords: head injuries; head trauma; skull fracture; temporal bone fracture; motor vehicles; traffic accidents; facial paralysis
Pengembangan Ekonomi Lokal Melalui Olahan Buah Nanas Di Desa Bedali Kecamatan Ngancar Kabupaten Kediri
Authors: Astuti Indah Yuni , Niam Muhammad Alfa , Handayani Tri 
Published: December 2019

Potensi Desa Bedali dalam aspek pertanian khususnya buah nanas menjadi potensi tersendiri. Mayoritas pekerjaan masyarakat desa Bedali Kecamatan Ngancar sebagai petani yang menghasilkan buah sirsak dan nanas. Melimpahnya buah nanas di desa Bedali menjadi salah satu alasan tujuan pengabdian kepada masyarakat ini. Salah satu produk olahan yang dihasilkan oleh ibu-ibu PKK di desa Bedali yaitu sari buah nanas. Permasalahan yang dihadapi oleh petani nanas di Desa Bedali adalah keterbatasan alat yang dimiliki serta kurangnya pengetahuan dan ketrampilan dalam mengolah buah nanas. Permasalahan lain yang dihadapi yaitu apabila jumlah buah nanas melimpah, sedangkan permintaan pasar rendah, maka buah nanas tidak akan laku, akibatnya buah nanas menjadi busuk dan apabila sudah busuk maka akan dibuang. Hal ini akan menyebabkan kerugian bagi petani. Sehingga, ibu ibu PKK yang juga menjadi petani buah nanas ini perlu mendapatkan perhatian untuk merubah mindset tidak hanya mampu bertani, tetapi juga mampu mengolah hasil pertanian. Metode yang dilaksanakan dalam kegiatan pengabdian masyarakat ini adalah memberikan pembinaan dan pelatihan dari aspek produksi dan aspek manajemen usaha. Dari program Pengabdian Masyarakat ini adalah pemberian pelatihan pengolahan buah nanas menjadi selai dan kue. Pelatihan pembukuan dan manajemen keuangan kepada ibu-ibu PKK dan pemberian peralatan mesin untuk menunjang produksi olahan minuman sari buah nanas.
Kebersihan Gigi Tiruan pada Lansia, Suatu Tinjauan Metode dan Bahan
Authors: Sari Kartika Indah , Dewi Warta , Jasrin Tadeus Arufan , Sumarsongko Taufik 
Published: December 2019

The use of removable dentures is one of the main approach to oral rehabilitation of partially or completely edentulous patient. Denture hygiene is an important things for the elderly as a vulnerable people. The purpose is to discribe both of the literature or recent study that related to denture hygiene in elderly. Removable denture got special attention cause accumulate food residue, bacterial biofilm, and calculus at the mucosa or denture interface. Recently, the presence of bacterial bioflim on denture was considered a risk factor for aspiration pneumonia in frail older people. In general, removable denture that is used acrylic based denture.The microporous surfaces of an acrylic denture provide a wide range of environments to support microorganisms that can threaten the health of patient. Knowing denture cleanser as apart of denture hygiene in elderly is an important things.
ANALYSIS OF THE APPLICATION OF BETAWI ARCHITECTURE IN CAFE DESIGN IN JAKARTAFROM THE ASPECT OF AFFORDABILITY IN CONSTRUCTION
Authors: Hendarti Religiana , Soebiyan Vivien , Milla Yanita 
Published: December 2019

This paper presents a study of the things that determine the implementation of Betawi architecture in a 'café' design for a community in Jakarta. The café for this community is a project to facilitate the activities of PKK women in Palmerah District, Jakarta. The majority of these communities are local residents who are also mostly Betawi ethnic, therefore, this design requires an affordable design, in the sense of a design that can represent local culture with the application of Betawi architecture that can meet limited funding. As is known, Betawi architecture is architecture that is rich in detail ornamentation and made of wood. The result of this design is a 'café' design that uses the most important Betawi ornament with a frame structure and iron construction.
INCREASED AWARENESS OF GREEN STRATEGY TO SUPPORT THE SUSTAINABILITY OF FOOD SMEs IN SOUTH MERUYA WEST JAKARTA
Authors: Hasibuan Sawarni , Rimawan Erry 
Published: December 2019

The government now is always trying some efforts and policy to support competitiveness of Small and Medium Enterprise (SMEs) in Indonesia. Food SMEs is one of SMEs which the quantity is very big and spreading in various location commonly near the housing and business center. Entrepreneurs of Food SMEs is also included in the entity to get attention from the government to increase the competitiveness. Environmental dynamics and care of city community of environmental problems encourage the food’s entrepreneurs to adopt green strategy in the process of production in order to get better image so that it can contribute to the sustainability of SMEs. One approach to be adopted is green productivity strategy. Green productivity strategy tries to take care of the environment by the entrepreneur not only from the environmental benefit but also financial benefit, because production process will be operating with minimal waste and efficiency of course will produce benefit financially. This activity has the purpose to increase awareness of green strategy implementation in Food SMEs in District South Meruya West Jakarta. Conducting the activity is done in one months. The approach used is to integrate lecturing, discussion and question answer, self assessment, and simulation. To measure effectivity of the activity have deployed questionnaire pre test and post test related to ability of knowledge, assessment, and analysis of opportunity and application of green strategy in the production chain Food SMEs. The outing target of the activity is increasing the knowledge and ability of SMEs entrepreneurs about the green implementation in production activity. The results of this training show that participants are able to identify the potential of solid waste on the production process that can be processed into value-added products.
Europejska walka z dezinformacjami i nielegalnymi treściami w sieci. Obrona jakości mediów w Unii Europejskiej i w Radzie Europy
Authors: Jaskiernia Alicja 
Published: December 2019

Unia Europejska i Rada Europy podejmują coraz bardziej kompleksowe działania w walce tzw. postprawdą oraz nielegalnymi treściami w sieci. Cel: Artykuł ma charakter przeglądowy. Celem jest ukazanie najważniejszych bieżących działań obu organizacji w obszarze polityki medialnej oraz ukazanie pierwszych efektów nakładanych na platformy internetowe zobowiązań. Drugi cel stanowi przegląd kluczowych tendencji na europejskim rynku audiowizualnym oraz przedstawienie aktualnych zmian w regulacjach unijnych, które dotyczą rynku mediów. Metoda: desk research (analiza ustawodawstwa, dokumentów i raportów). Wyniki i wnioski: Przegląd pozwala uzyskać obraz głównych wyzwań, z jakimi muszą mierzyć się organizacje paneuropejskie (oraz państwa członkowskie) w związku z dynamiką zmian w ekosystemie medialnym. Wartość poznawcza: Artykuł umożliwia zapoznanie się z aktualną działalnością Rady Europy i Unii Europejskiej w obszarze walki z dezinformacją, a także śledzenie najnowszych regulacji dotyczących mediów oraz zmian na rynku audiowizualnym.
PENGARUH PEMBERIAN CUKA APEL (Malus sylvestris Mill) VARIETAS MANALAGI TERHADAP KADAR TRIGLISERIDA DARAH TIKUS PUTIH (Rattus norvegicus) JANTAN GALUR WISTAR YANG DIBERI DIET TINGGI LEMAK DAN DIINDUKSI ALOKSAN
Authors: Yuliyanti Yuliyanti , Tjandra A. Sareh Arjono , Setianingsih Herin 
Published: December 2019

Hypertriglyceridemia is one of the risk factors for cardiovascular disease and acute pancreatitis (Pejic and Lee, 2006; Brahm and Hegele, 2013; Afari et al., 2015). One way to treat it by using the apple vinegar (Malus sylvestris Mill) of manalagi varieties which contained high antioxidants and acetic acid as well as more durable than the fresh fruit. The study aimed to know the effect of the Apple vinegar of manalagi varieties (Malus sylvestris Mill) to blood triglyceride levels of white male rats (Rattus norvegicus) wistar strain with high-fat diet and induced by alloxan. The study used the post test only control group design, with 24 rats samples which were divided into three groups. The negative control group (K0) only received standard diet for 24 days, the positive control group (K1) received a high-fat diet for 7 days and induced by alloxan with doses of 90 mg/kgBW of rats, and treatment group (K2) was given a high-fat diet for 7 days, induced by alloxan and received the apple vinegar (Malus sylvestris Mill) of manalagi varieties with a dose of 0,27 ml/100 g BW of rats for 14 days. The results by using one way ANOVA showed the significance value of the data p = 0,974 (p>0,05) so that there is no significant difference of the mean of blood triglycerides levels between K0, K1 and K2. This study concluded that apple vinegar (Malus sylvestris Mill) of manalagi varieties can not significantly lower the blood triglyceride levels of white male rats (Rattus norvegicus) wistar strain with high-fat diet and induced by alloxan. Further study about apple vinegar of manalagi varieties is required to define its effects. Keywords—: High-Fat Diet, Alloxan, Triglyceride, Apple Vinegar (Malus sylvestris Mill) of Manalagi Varieties
A Need Analysis of ESP For Office Administration Program at SMK Negeri 2 Purworejo in the Academic Year of 2018/2019
Authors: Mentari Putri Yuni 
Published: December 2019

The objectives of the study are to describe the textbooks used by English teachers at present and its quality for Office Administration program; to explain to what extend do the teachers and the students need of ESP-Content based English materials in their English textbook and to describeEnglish skills and the material of ESP for Office Administration students. To get the data for the study, two sets of questionnaire were used. First was questionnaire for teacher, second questionnaire for students. The steps for analyzing the data were (1) Selecting the data from the result of the questionnaire ,(2) Identifying the data about student’s need in the ESP material and the English textbook, (3) Classifying the data from the teacher and student’s answer,(4) Calculating percentage of answers in the questionnaire,(5) Making conclusion.The result of the study shows that teacher used two English textbooks in teaching English. The English textbooks have a good quality, in term of the learners’ activities, the picture and illustration. However the material in the textbook was not appropriate with the student need in Office Administration Program. The students and the teacher need English textbook which contains with the ESP. The English textbook should contain the items based the students need and theEnglish skill needed by the students in Office Administration Program.