bluuebunny/crossref_metadata_2025_split huggingface dataset:
Seasonal Migration in the Aphid Genus Stomaphis (Hemiptera: Aphididae): Discovery of Host Alternation Between Woody Plants in Subfamily Lachninae
Authors: Yamamoto Tetsuya , Hattori Mitsuru , Itino Takao 
Published: September 2020

AbstractAbout 10% of aphid species show host alternation. These aphids migrate between primary and secondary host plant species in spring and autumn. Host alternation has not been observed in subfamily Lachninae, although it has been suggested on the basis of circumstantial evidence that Stomaphis japonica (Takahashi) may alternate its host between Quercus serrata (Murray) and Quercus acutissima (Carruth). However, a molecular phylogenetic study has indicated that the Stomaphis individuals feeding on these two plant species belong to two different lineages and aphids feeding on Q. acutissima and Pinus densiflora (Sieb. & Zucc.) belong to the same lineage. Here, we examined host alternation in Stomaphis species by comparing molecular phylogenetic identities, morphological features, and life cycles. The molecular analysis and morphological examination showed that aphids feeding on Q. acutissima were the same as those feeding on P. densiflora, whereas aphids feeding on Q. serrata were different from those feeding on Q. acutissima or on P. densiflora. Furthermore, winged aphids were observed on both Q. acutissima and P. densiflora in autumn, but we did not observe winged aphids on Q. serrata. These results indicate that Stomaphis (Walker) individuals feeding on Q. serrata and Q. acutissima belong to two species, one that feeds year-round on Q. serrata, and another, heteroecious species that feeds on P. densiflora as a primary host and on Q. acutissima as a secondary host. This study documents host alternation in subfamily Lachninae for the first time and discusses the acquisition of host alternation by Stomaphis from evolutionary and ecological perspectives.
Commerce of Edible Insects in the State of Morelos, Mexico
Authors: Pino Moreno José Manuel , Reyes-Prado Humberto 
Published: September 2020

AbstractThe present study deals with the taxonomical analysis of the edible insects commercialized in the state of Morelos, Mexico. We have recorded two species under the order Orthoptera and four species under Hemiptera–Heteroptera. Our work revealed that grasshoppers and small hemipterans known as ‘jumiles’ are the two main insects sold in the markets of the state of Morelos. We also discuss insects’ prices and their economic importance for the livelihood improvement of the local people connected to the edible insect trade.
Safety and pharmacokinetics of polatuzumab vedotin in Japanese patients with relapsed/refractory B-cell non-Hodgkin lymphoma: a phase 1 dose-escalation study
Authors: Kinoshita Tomohiro , Hatake Kiyohiko , Yamamoto Kazuhito , Higuchi Yusuke , Murakami Satsuki , Terui Yasuhito , Yokoyama Masahiro , Maruyama Dai , Makita Shinichi , Hida Yukari , Saito Tomohisa , Tobinai Kensei 
Published: September 2020

Abstract

Objective
A phase 1 dose-escalation study of polatuzumab vedotin (pola) was conducted to assess safety, pharmacokinetics and preliminary antitumor activity of pola in Japanese patients with relapsed/refractory B-cell non-Hodgkin lymphoma.

Methods
Patients received pola (1.0 or 1.8 mg/kg) intravenously every 21 days until disease progression or intolerance. Intra-patient dose escalation was prohibited. Tolerability was determined by the standard 3 + 3 rule. Blood sampling was performed to characterize pharmacokinetics. Antitumor activity was evaluated through computed tomography and bone marrow sampling.

Results
Four patients received pola 1.0 mg/kg; three received 1.8 mg/kg. Patients had follicular lymphoma (n = 4) or diffuse large B-cell lymphoma (n = 3), median age of 62 years, received a median of 3 prior therapies; six were female. Pola was well tolerated in both cohorts, with no dose-limiting toxicities observed. The most common adverse event was peripheral sensory neuropathy (n = 4). Grade 3 adverse events were cholecystitis and neutrophil count decreased (one each; both 1.0 mg/kg), and syncope and cataract (one each; both 1.8 mg/kg). The plasma half-life of antibody-conjugate monomethyl auristatin E was 4.43–7.98 days, and systemic exposure of unconjugated monomethyl auristatin E was limited in both cohorts. Four patients achieved objective responses (three complete, one partial) without disease progression during the study.

Conclusions
This phase 1 dose-escalation study demonstrated that pola has an acceptable safety profile and offers encouraging antitumor activity to Japanese patients with relapsed/refractory B-cell non-Hodgkin lymphoma. Pola 1.8 mg/kg, the recommended phase 2 dose, was tolerable in Japanese patients.
Prognosis and surgical outcome of soft tissue sarcoma with malignant fungating wounds
Authors: Okajima Koichi , Kobayashi Hiroshi , Okuma Tomotake , Arai Sho , Zhang Liuzhe , Hirai Toshihide , Ishibashi Yuki , Ikegami Masachika , Shinoda Yusuke , Akiyama Toru , Goto Takahiro , Tanaka Sakae 
Published: September 2020

Abstract

Objective
Malignant fungating wounds are ulcerating tumors that infiltrate the overlying skin. Little evidence exists regarding the prognosis or treatment of malignant fungating wound in soft tissue sarcoma. This study aimed to reveal the prognosis and outcome of surgical treatment of malignant fungating wound in soft tissue sarcoma.

Methods
We retrospectively reviewed 26 patients with malignant fungating wound in high-grade soft tissue sarcoma between 2005 and 2018. The patients’ characteristics, treatments, surgical wound complications, local recurrences and prognoses were analyzed. Overall survival was analyzed using the Kaplan–Meier method and compared with that of the control cohort, consisting of 236 consecutive patients with non-malignant fungating wound high-grade soft tissue sarcoma treated during the same period.

Results
Among the 26 patients, undifferentiated pleomorphic sarcoma was the most common subtype. Twenty-three patients, including 20 (87%) and 3 (13%), underwent limb-salvage surgery and amputation, respectively. Among the 20 patients who underwent limb-salvage surgery, 4 (20%) had surgical wound complications, which required additional surgical procedures. Excluding the patients who underwent palliative surgery, local recurrence occurred in 2 patients (11%). The 5-year overall survival rate for all high-grade malignant fungating wound and non-malignant fungating wound patients was 26.0 and 67.3% (P &lt; 0.0001), respectively.

Conclusions
Malignant fungating wounds in soft tissue sarcoma were significantly associated with a poor prognosis; however, the incidence of surgical complications and local recurrence after limb-salvage surgery was comparable to that of general soft tissue sarcoma cases. Limb-salvage surgery should be considered, if possible, to preserve the patient’s quality of life because of the dismal prognosis of patients with malignant fungating wound in soft tissue sarcoma.
Redescription of Brennanacarus annereauxi (Trombidiformes: Trombiculidae) With New Records for Uruguay
Authors: Bassini-Silva Ricardo , Huang-Bastos Matheus , Welbourn Cal , Ochoa Ron , Barros-Battesti Darci Moraes , de Castro Jacinavicius Fernando 
Published: September 2020

AbstractBrennanacarus annereauxi (Brennan and Yunker, 1969) was described from Venezuela parasitizing the greater spear-nosed bat. The monotypic genus Brennanacarus Goff, Yunker and Wheeler, 1987 is a replacement name for Nasicola Brennan and Yunker, 1969, which was preoccupied by Nasicola Yamaguti, 1968. Here, we redescribe the genus Brennanacarus and the type species B. annereauxi. Also, this is the first record of this species, as well as the second record of any chigger, for Uruguay.
Tophaceous gout in the sternoclavicular joint
Authors: Sahdev Nikhil , Smelt Jeremy , Avila Zoe , Tennent Duncan , Hunt Ian 
Published: September 2020

Abstract
Gouty arthritis commonly occurs in peripheral synovial joints and involvement in the thoracic cage is extremely rare. We report a case of a 52-year-old presenting with a painful bony mass in her sternal notch. Imaging demonstrated a lobulated calcified and necrotic lesion causing mass effect on adjacent structures. Excision biopsy confirmed a benign cystic lesion with amorphous material containing needle-shaped refractile crystals suggestive of uric acid. Tophaceous gout was diagnosed. Presence of a periarticular mass with characteristics of gout should warrant investigation with biopsy and imaging. This can prevent misdiagnosis of malignancy and avoid unnecessary surgery in asymptomatic lesions.
Sporothrix brasiliensis on cats with skin ulcers in Southern Brazil
Authors: Spanamberg Andréia , Araujo Ricardo , Ravazzolo Ana Paulo , Driemeier David , Driemeier Rosane Maria Sordi , Ferreiro Laerte 
Published: September 2020

Abstract
Sporotrichosis is a disease caused by thermally dimorphic fungi belonging to the Sporothrix schenckii complex. We report the occurrence of multiple cases of sporotrichosis in cats in Porto Alegre (RS), Brazil, from 2015 to 2019. A set of 21 fungal isolates were collected and the partial sequence of calmodulin (CAL) gene compared. All isolates were identified at phenotypic and molecular level as S. brasiliensis. Phylogenetic analysis showed the isolates clustered in two distinct groups, suggesting the presence of multiple genetic variants of S. brasiliensis in the region.
The synchrotron maser emission from relativistic magnetized shocks: dependence on the pre-shock temperature
Authors: Babul Aliya-Nur , Sironi Lorenzo 
Published: September 2020

ABSTRACT
Electromagnetic precursor waves generated by the synchrotron maser instability at relativistic magnetized shocks have been recently invoked to explain the coherent radio emission of fast radio bursts. By means of 2D particle-in-cell simulations, we explore the properties of the precursor waves in relativistic electron–positron perpendicular shocks as a function of the pre-shock magnetization σ ≳ 1 (i.e. the ratio of incoming Poynting flux to particle energy flux) and thermal spread Δγ ≡ kT/mc2 = 10−5−10−1. We measure the fraction fξ of total incoming energy that is converted into precursor waves, as computed in the post-shock frame. At fixed magnetization, we find that fξ is nearly independent of temperature as long as Δγ ≲ 10−1.5 (with only a modest decrease of a factor of 3 from Δγ = 10−5 to Δγ = 10−1.5), but it drops by nearly two orders of magnitude for Δγ ≳ 10−1. At fixed temperature, the scaling with magnetization $f_\xi \sim 10^{-3}\, \sigma ^{-1}$ is consistent with our earlier 1D results. For our reference σ = 1, the power spectrum of precursor waves is relatively broad (fractional width ∼1 − 3) for cold temperatures, whereas it shows pronounced line-like features with fractional width ∼0.2 for 10−3 ≲ Δγ ≲ 10−1.5. For σ ≳ 1, the precursor waves are beamed within an angle ≃σ−1/2 from the shock normal (as measured in the post-shock frame), as required so they can outrun the shock. Our results can provide physically grounded inputs for FRB emission models based on maser emission from relativistic shocks.
Interstellar scintillation of an extreme scintillator: PKS B1144−379
Authors: Said N M M , Ellingsen S P , Bignall H E , Shabala S , McCallum J N , Reynolds C 
Published: September 2020

ABSTRACT
The University of Tasmania Ceduna radio telescope has been used to investigate rapid variability in the radio flux density of the BL Lac object PKS B1144−379 at 6.7 GHz. High-cadence monitoring of this extreme scintillator was carried out over a period of approximately 9 yr, between 2003 and 2011. We have used structure functions created from the intensity time-series to determine the characteristic time-scale of the variability. The characteristic time-scale is consistently observed to increase during certain periods of each year, demonstrating the annual cycle expected for scintillation through an interstellar scattering screen. The best-fitting annual cycle model for each year suggests that the scintillation pattern has an anisotropic structure and that the upper limit of its scattering screen is at a distance of ∼0.84 kpc. Higher anisotropy in some of the annual cycle fits suggests that changes in the intrinsic source structure might be influencing the variability time-scale. We found a prominent annual cycle is only present in the variability time-scale for certain years, where other evidence suggests that the core is compact. From our measurements, we calculated that the core angular size varied between 5.65 and 15.90 μas (0.05–0.13 pc). The core component was found to be at its most compact during two flares in the total flux density, which were observed in 2005 and 2008. We conclude that the long-term variability in the radio flux density of PKS B1144−379 is due to intrinsic changes in the source and that these affect our ability to measure an annual cycle in its variability time-scale.
Discovery of a hot ultramassive rapidly rotating DBA white dwarf
Authors: Pshirkov M S , Dodin A V , Belinski A A , Zheltoukhov S G , Fedoteva A A , Voziakova O V , Potanin S A , Blinnikov S I , Postnov K A 
Published: September 2020

ABSTRACT
We report the discovery of a nearby massive white dwarf with He–H atmosphere. The white dwarf is located at a distance of 74.5 ± 0.9 pc. Its radius, mass, effective temperature, H/He ratio, and age are R = 2500 ± 100 km, M = 1.33 ± 0.01 $\rm M_{\odot }$, Teff = 31 200 ± 1200 K, H/He ∼ 0.1, and 330 ± 40 Myr, respectively. The observed spectrum is redshifted by Vr = +240 ± 15 km s−1, which is mostly attributed to the gravitational redshift. The white dwarf shows a regular stable photometric variability with amplitude Δg ≈ 0.06m and period P = 353.456 s suggesting rapid rotation. This massive, hot, and rapidly rotating white dwarf is likely to originate from the merging of close binary white dwarf system that avoided explosion in a thermonuclear Type Ia supernova.
Eighteen Coral Genomes Reveal the Evolutionary Origin of Acropora Strategies to Accommodate Environmental Changes
Authors: Shinzato Chuya , Khalturin Konstantin , Inoue Jun , Zayasu Yuna , Kanda Miyuki , Kawamitsu Mayumi , Yoshioka Yuki , Yamashita Hiroshi , Suzuki Go , Satoh Noriyuki 
Published: September 2020

Abstract
The genus Acropora comprises the most diverse and abundant scleractinian corals (Anthozoa, Cnidaria) in coral reefs, the most diverse marine ecosystems on Earth. However, the genetic basis for the success and wide distribution of Acropora are unknown. Here, we sequenced complete genomes of 15 Acropora species and 3 other acroporid taxa belonging to the genera Montipora and Astreopora to examine genomic novelties that explain their evolutionary success. We successfully obtained reasonable draft genomes of all 18 species. Molecular dating indicates that the Acropora ancestor survived warm periods without sea ice from the mid or late Cretaceous to the Early Eocene and that diversification of Acropora may have been enhanced by subsequent cooling periods. In general, the scleractinian gene repertoire is highly conserved; however, coral- or cnidarian-specific possible stress response genes are tandemly duplicated in Acropora. Enzymes that cleave dimethlysulfonioproprionate into dimethyl sulfide, which promotes cloud formation and combats greenhouse gasses, are the most duplicated genes in the Acropora ancestor. These may have been acquired by horizontal gene transfer from algal symbionts belonging to the family Symbiodiniaceae, or from coccolithophores, suggesting that although functions of this enzyme in Acropora are unclear, Acropora may have survived warmer marine environments in the past by enhancing cloud formation. In addition, possible antimicrobial peptides and symbiosis-related genes are under positive selection in Acropora, perhaps enabling adaptation to diverse environments. Our results suggest unique Acropora adaptations to ancient, warm marine environments and provide insights into its capacity to adjust to rising seawater temperatures.
Comparative study of preimplantation development following distinct assisted oocyte activation protocols in a PLC-zeta knockout mouse model
Authors: Ferrer-Buitrago M , Tilleman L , Thys V , Hachem A , Boel A , Van Nieuwerburgh F , Deforce D , Leybaert L , De Sutter P , Parrington J , Heindryckx B 
Published: September 2020

AbstractMammalian fertilization encompasses a series of Ca2+ oscillations initiated by the sperm factor phospholipase C zeta (PLCζ). Some studies have shown that altering the Ca2+ oscillatory regime at fertilization affects preimplantation blastocyst development. However, assisted oocyte activation (AOA) protocols can induce oocyte activation in a manner that diverges profoundly from the physiological Ca2+ profiling. In our study, we used the newly developed PLCζ-null sperm to investigate the independent effect of AOA on mouse preimplantation embryogenesis. Based on previous findings, we hypothesized that AOA protocols with Ca2+ oscillatory responses might improve blastocyst formation rates and differing Ca2+ profiles might alter blastocyst transcriptomes. A total of 326 MII B6D2F1-oocytes were used to describe Ca2+ profiles and to compare embryonic development and individual blastocyst transcriptomes between four control conditions: C1 (in-vivo fertilization), C2 (ICSI control sperm), C3 (parthenogenesis) and C4 (ICSI-PLCζ-KO sperm) and four AOA groups: AOA1 (human recombinant PLCζ), AOA2 (Sr2+), AOA3 (ionomycin) and AOA4 (TPEN). All groups revealed remarkable variations in their Ca2+ profiles; however, oocyte activation rates were comparable between the controls (91.1% ± 13.8%) and AOA (86.9% ± 11.1%) groups. AOA methods which enable Ca2+ oscillatory responses (AOA1: 41% and AOA2: 75%) or single Ca2+ transients (AOA3: 50%) showed no significantly different blastocyst rates compared to ICSI control group (C2: 70%). In contrast, we observed a significant decrease in compaction (53% vs. 83%) and blastocyst rates (41% vs. 70%) in the absence of an initial Ca2+ trigger (AOA4) compared with the C2 group. Transcription profiles did not identify significant differences in gene expression levels between the ICSI control group (C2) and the four AOA groups.
Revisiting the action of steroids and triterpenoids on the human sperm Ca2+ channel CatSper
Authors: Rehfeld Anders 
Published: September 2020

ABSTRACT
The sperm-specific Ca2+ channel CatSper (cation channel of sperm) is vital for male fertility. Contradictory findings have been published on the regulation of human CatSper by the endogenous steroids estradiol, testosterone and hydrocortisone, as well as the plant triterpenoids, lupeol and pristimerin. The aim of this study was to elucidate this controversy by investigating the action of these steroids and plant triterpenoids on human CatSper using population-based Ca2+-fluorimetric measurements, the specific CatSper-inhibitor RU1968 and a functional test assessing the CatSper-dependent penetration of human sperm cells into methylcellulose. Estradiol, testosterone and hydrocortisone were found to induce Ca2+-signals in human sperm cells with EC50 values in the lower μM range. By employing the specific CatSper-inhibitor RU1968, all three steroids were shown to induce Ca2+-signals through an action on CatSper, similar to progesterone. The steroids were found to dose-dependently inhibit subsequent progesterone-induced Ca2+-signals with IC50 values in the lower μM range. Additionally, the three steroids were found to significantly increase the penetration of human sperm cells into methylcellulose, similar to the effect of progesterone. The two plant triterpenoids, lupeol and pristimerin, were unable to inhibit progesterone-induced Ca2+-signals, whereas the CatSper-inhibitor RU1968 strongly inhibited progesterone-induced Ca2+-signals. In conclusion, this study supports the claim that the steroids estradiol, testosterone and hydrocortisone act agonistically on CatSper in human sperm cells, thereby mimicking the effect of progesterone, and that lupeol and pristimerin do not act as inhibitors of human CatSper.
Ribosomal stalk proteins RPLP1 and RPLP2 promote biogenesis of flaviviral and cellular multi-pass transmembrane proteins
Authors: Campos Rafael K , Wijeratne H R Sagara , Shah Premal , Garcia-Blanco Mariano A , Bradrick Shelton S 
Published: September 2020

AbstractThe ribosomal stalk proteins, RPLP1 and RPLP2 (RPLP1/2), which form the ancient ribosomal stalk, were discovered decades ago but their functions remain mysterious. We had previously shown that RPLP1/2 are exquisitely required for replication of dengue virus (DENV) and other mosquito-borne flaviviruses. Here, we show that RPLP1/2 function to relieve ribosome pausing within the DENV envelope coding sequence, leading to enhanced protein stability. We evaluated viral and cellular translation in RPLP1/2-depleted cells using ribosome profiling and found that ribosomes pause in the sequence coding for the N-terminus of the envelope protein, immediately downstream of sequences encoding two adjacent transmembrane domains (TMDs). We also find that RPLP1/2 depletion impacts a ribosome density for a small subset of cellular mRNAs. Importantly, the polarity of ribosomes on mRNAs encoding multiple TMDs was disproportionately affected by RPLP1/2 knockdown, implying a role for RPLP1/2 in multi-pass transmembrane protein biogenesis. These analyses of viral and host RNAs converge to implicate RPLP1/2 as functionally important for ribosomes to elongate through ORFs encoding multiple TMDs. We suggest that the effect of RPLP1/2 at TMD associated pauses is mediated by improving the efficiency of co-translational folding and subsequent protein stability.
CYP24A1 and KL polymorphisms are associated with the extent of vascular calcification but do not improve prediction of cardiovascular events
Authors: Solache-Berrocal Guillermo , Rolle-Sóñora Valeria , Martín-Fernández Noelia , Cambray Serafí , Valdivielso José Manuel , Rodríguez Isabel 
Published: September 2020

Abstract

Background
Novel ways of determining cardiovascular risk are needed as a consequence of population ageing and the increased prevalence of chronic kidney disease (CKD), both of which favour vascular calcification. Since the formation of arterial calcium deposits has a genetic component, single nucleotide polymorphisms (SNPs) could predict cardiovascular events.

Methods
A selection of 1927 CKD patients and controls recruited by the NEFRONA study were genotyped for 60 SNPs from 22 candidate genes. A calcium score was calculated from the echogenicity of arterial atherosclerotic plaques and the presence of cardiovascular events during a 4-year period was recorded. Association of SNPs with the calcium score was identified by multiple linear regression models and their capacity to predict events was assessed by means of Cox proportional hazards regression and receiver operating characteristics curves.

Results
Two variants, rs2296241 of CYP24A1 and rs495392 of KL, were associated with the calcium score. Despite this, only heterozygotes for rs495392 had a lower risk of suffering an event compared with homozygotes for the major allele {hazard ratio (HR) 0.67 [95% confidence interval (CI) 0.48−0.93]}. Of note, the calcium score was associated with an increased risk of cardiovascular events [HR 1.71 (95% CI 1.35−2.17)]. The addition of the rs495392 genotype to classical cardiovascular risk factors did not increase the predictive power [area under the curve (AUC) 71.3 (95% CI 61.1−85.5) versus 71.4 (61.5−81.4)].

Conclusions
Polymorphisms of CYP24A1 and KL are associated with the extent of calcification but do not predict cardiovascular events. However, the echogenic determination of the extent of calcium deposits seems a promising non-irradiating method for the scoring of calcification in high-risk populations.