bluuebunny/crossref_metadata_2025_split huggingface dataset:
Temporal cross-correlations between air pollutants and outpatient visits for respiratory and circulatory system diseases in Fuzhou, China
Authors: Jiang Yu , Chen Jiedong , Wu Chuancheng , Lin Xin , Zhou Quan , Ji Shumi , Yang Shuangfeng , Zhang Xiaoyang , Liu baoying 
Published: March 2020

Abstract
Background: Previous studies have suggested that there is an association between air pollutants and circulatory and respiratory diseases, but relatively few have analyzed the association between air pollutants and outpatient visits considering mortality, hospitalization rates, etc., especially areas with relatively good air quality. Therefore, we conducted this study to research the association between air pollutants and outpatient visits in Fuzhou, China. Methods: We used a generalized linear Poisson model to study the association between air pollution and outpatient visits for respiratory and circulatory diseases during 2016-2018 in Fuzhou, China. Results: In the single pollutant model, nitrogen dioxide (NO2) had a significant effect. For lag day 0 to lag day 5, the effect decreased with every 10 µg/L increase in NO2. Daily maximum 8-h mean ozone (8-h O3) and upper respiratory outpatient visits were positively associated during the cold period [lag2, excess risk (ER) (95% confidence interval (CI)): 1.68% (0.44%-2.94%)], while 8-h O3 and respiratory disease were positively associated during the warm period [lag5, ER (95% CI): 1.10% (0.11%-2.10%) and lag4, ER (95% CI): 1.02% (0.032%-2.02%)]. Similarly, particulate matter (PM) with an average aerodynamic diameter of less than 10 μm (PM10) and lower respiratory diseases were positively associated during the warm period [lag0, ER (95% CI): 1.68% (0.44%-2.94%)]. When the concentration of 8-h O3 was higher than 100 μg/L, there was a positive effect on circulatory [lag5, ER (95% CI): 2.83% (0.65%-5.06%)], respiratory [lag5, ER (95% CI): 2.47% (0.85%-4.11%)] and upper respiratory [lag5, ER (95% CI): 3.06% (1.38%-4.77%)] outpatient visits. The variation in 8-h O3 changed slightly when we adjusted for the other air pollutants, and after adjusting for 8-h O3, the ERs of the other air pollutants changed slightly. After adjusting for PM with an average aerodynamic diameter of less than 2.5 μm (PM2.5), the ERs of the other air pollutants increased, and after adjusting for NO2, the ER of PM decreased. Conclusion: Exposure to ambient NO2, O3, PM2.5 and PM10 was associated with an increase in respiratory and circulatory system-related outpatient visits in Fuzhou, China.
Manganese Stress in Flue-cured Tobacco: Biochemical Responses
Authors: zou congming , Li Yan , Deng Xiaopeng , Huang Wei , Pu Guorui , Zhang Xiaohai , Ren Ke , Chen Yi , Jin Yan , Chen Yanjie , Li Junying , Lin Zhonglong 
Published: March 2020

Abstract
The authors have withdrawn this preprint from Research Square.
Be Quiet and Man Up: A Qualitative Questionnaire Study into Fathers Who Witnessed Their Partner’s Birth Trauma
Authors: Daniels Emily , Arden-Close Emily , Mayers Andrew 
Published: March 2020

Abstract
Background:
Research focusing on paternal mental health is limited, especially regarding the impact of the experience of poor mental health in the perinatal period. For example, little is known about the experiences of fathers who witness their partner’s traumatic birth and the subsequent impact on their mental health. Therefore, the aim of this study was to explore fathers’ experiences of witnessing a traumatic birth, how these experiences impacted their wellbeing, and what support they received during and following the traumatic birth.
Methods:
Sixty-one fathers were recruited via targeted social media to complete an anonymous online qualitative questionnaire regarding their birth trauma experience. Eligible participants were fathers aged eighteen or over, resided in the UK and had witnessed their partner’s traumatic birth (that did not result in loss of life). Thematic analysis was used to analyse the questionnaire data.
Results:
Three main themes were identified: ‘fathers’ understanding of the experience’ (subthemes: nothing can prepare you for it; merely a passenger; mixed experiences with staff; not about me); ‘life after birth trauma’ (subthemes: manhood after birth; inability to be happy; impact on relationships); and ‘the support fathers received vs what they wanted’ (subthemes: prenatal support; birth support; and postnatal support).
Conclusions:
Fathers reported that witnessing their partner’s traumatic birth had a significant impact on them. They felt this affected their mental health and relationships long into the postnatal period. However, there is no nationally recognised support in place for fathers to use as a result of their experiences. The participants attributed this to being perceived as less important than women in the postnatal period, and maternity services’ perceptions of the father more generally. Implications include ensuring support is available for both the mother and father following a traumatic birth, with additional staff training geared towards the father’s role.
Identification and Analysis of the FAD Gene Family in Walnuts (Juglans regia L.) Based on Transcriptome Data
Authors: Liu Kai , Zhao Shugang , Wang Shuang , Wang Hongxia , Zhang Zhihua 
Published: March 2020

Abstract
Background: Walnut kernels contain a large amount of unsaturated fatty acids, such as linoleic acid and linolenic acid, which are essential fatty acids for humans and have important effects on growth and health. The main function of fatty acid desaturase (FAD), which is widely distributed in organisms, is to remove hydrogen from carbon chains in the biosynthesis of unsaturated fatty acids to generate C=C bonds. Results: By performing a series of bioinformatics analysis, 24 members of the JrFAD gene family were identified from the genome database of walnut, and then compared with the homologous genes from Arabidopsis. Phylogenetic analysis showed that JrFADs were classified into four subfamilies: the SAD desaturase subfamily, Δ7/Δ9 desaturase subfamily, Δ12/ω-3 desaturase subfamily and "front-end" desaturase subfamily. Meanwhile, the expression of fatty acid synthesis genes in walnut kernels at different developmental stages was analysed by transcriptome sequencing, with expression of JrFAD3-1, which encodes an enzyme involved in linolenic acid synthesis, being particularly prominent. The relative expression level of JrFAD3-1 changed dramatically with the kernel development stages and exhibited a Bell-Shaped Curve. A significant positive correlation was observed between the expression of JrFAD3-1 during 70-100 DAF (Days after flowering) and the content of alpha-linolenic acid during 100-130 DAF, with a correlation coefficient of 0.991. Additionally, JrFAD3-1 was proved closely related to homologous genes in Betula pendula and Corylus heterophylla, indicating that the conserved structure of FADs is consistent with classical plant taxonomy. Conclusion: Twenty-four members JrFADs in walnut were identified and classified into four subfamilies. JrFAD3-1 may play significant roles in the biosynthesis of polyunsaturated fatty acids in walnut.
Higher fatty liver index is associated with increased risk of new onset heart failure in healthy adults: A nationwide population-based study in Korea
Authors: Roh Jae-Hyung , Park Jae-Hyeong , Lee Hanbyul , Yoon Yong-Hoon , Kim Minsu , Kim Yong-Giun , Park Gyung-Min , Lee Jae-Hwan , Seong In-Whan 
Published: March 2020

Abstract
Background Heart failure (HF) is relatively common cardiovascular disease with high mortality and morbidity. Although it is associated with many cardiovascular risk factors, the association between nonalcoholic fatty liver disease (NAFLD), the most common chronic liver disease, and HF has not been evaluated in a large-scale cohort study. Thus, we evaluated the ability of the fatty liver Index (FLI), a surrogate marker of NAFLD, to predict the development of HF in healthy individuals. Methods We analyzed the association between the FLI and new-onset HF with multivariate Cox proportional-hazards models in 308,578 healthy persons without comorbidities who underwent the National Health check-ups in the republic of Korea from 2009 to 2014. Results A total of 2,532 subjects (0.8%) were newly diagnosed with HF during the study period (a median of 5.4 years). We categorized our subjects into quartile groups according to FLI (Q1, 0-4.9; Q2, 5.0-12.5; Q3, 12.6-31.0; and Q4, >31.0). The cumulative incidence of HF was significantly higher in the highest FLI group than in the lowest FLI group (Q1, 307 [0.4%] and Q4, 890 [1.2%]; P<0.001). Adjusted hazard ratio (HRs) indicated that the highest FLI group was independently associated with an increased risk for HF (HR between Q4 and Q1, 2.709; 95% confidence interval=2.380-3.085; P<0.001). FLI was significantly associated with an increased risk of new-onset HF regardless of their baseline characteristics.Conclusions Higher FLI was independently associated with increased risk of HF in a healthy Korean population.
Combined extracorporeal shock wave lithotripsy and endoscopic treatment for pain in chronic pancreatitis (SCHOKE Trial): study protocol for a randomized sham-controlled trial
Authors: Olesen Søren Schou , Drewes Asbjørn M. , Maragoni Rajesh Goud , Tandan Manu , Lakhtakia Sundeep , Ramchandani Mohan , Rao G V , Reddy D Nageshwar , Talukdar Rupjyoti 
Published: March 2020

Abstract
Background: Pain is the primary symptom of chronic pancreatitis (CP) and remains a considerable therapeutic challenge. In patients with obstruction of the pancreatic duct, including stones and strictures, endoscopic treatment with or without preceding extracorporeal shock wave lithotripsy (ESWL) have been used for pancreatic duct decompression. The rationale for these procedures is based on the assumption that obstruction of the pancreatic duct leads to ductal hypertension and pain. However, clinical pain symptoms correlate poorly with pancreatic duct morphology and the evidence for pancreatic duct decompression as an effective treatment for pain is based on case-series and comparison between different procedures, while no randomised prospective sham-controlled trials are currently available. The SCHOKE trial is a randomized sham-controlled trial designed to determine if pancreatic duct decompression is an effective treatment for pain in patients with CP.
Methods: The SCHOKE trial is a randomized, single-blinded, parallel-group, sham-controlled trial designed to evaluate the effect of combined ESWL and endoscopic treatment for pain in patients with CP. In total, 106 adult patients with painful CP and pancreatic duct obstruction will be randomized to combined ESWL and subsequent endoscopic treatment or corresponding sham procedures. The primary outcome is pain relief during the 3 months post-randomization period as documented in a pain diary. Secondary outcomes include quality of life and functional scores, patient’s global impression of change, change in use of analgesics, frequency of hospitalization and complications. Standard follow-up is at 3 and 6 months after randomization. In an experimental sub-study, quantitative sensory testing obtained before and after intervention will be used to obtain information on central pain processing and to develop models for prediction of treatment outcome.
Discussion: The SCHOKE trial investigates if pancreatic duct decompression, obtained by combined ESWL and endoscopic treatment, is effective for pain treatment in patients with CP.
Efficacy, safety, and tolerability of combined pirfenidone and N-acetylcysteine therapy: A systematic review and meta-analysis
Authors: Shi Hanyu , Yin Dawei , Bonella Francesco , Kreuter Michael , Oltmanns Ute , Li Xuren , Peng Shouchun , Wei Luqing 
Published: March 2020

Abstract
Background: While antifibrotic drugs significantly decrease lung function decline in idiopathic pulmonary fibrosis (IPF), there is still an unmet need to halt disease progression. Antioxidative therapy with N-acetylcysteine (NAC) is considered a potential additional therapy that can be combined with antifibrotics in some patients in clinical practice. However, data on the efficacy, tolerability, and safety of this combination are scarce. We performed a systematic review and meta-analysis to appraise the safety, tolerability, and efficacy of the combination compared to treatment with pirfenidone alone.
Methods: We systematically reviewed all the published studies with combined pirfenidone (PFD) and NAC (PFD+NAC) treatment in IPF patients. The primary outcomes referred to decline in pulmonary function tests (PFTs) and the rates of IPF patients with side effects.
Results: In the meta-analysis, 6 studies with 319 total IPF patients were included. The PFD+NAC group was comparable to the PFD alone group in terms of the predicted forced vital capacity (FVC%) and predicted diffusion capacity for carbon monoxide (DLco%) from treatment start to week 24. Side effects and treatment discontinuation rates were also comparable in both groups.
Conclusion: This systematic review and meta-analysis suggests that combination with NAC does not alter the efficacy, safety, or tolerability of PFD in comparison to PFD alone in IPF patients.
Association Analysis between SNPs in LATS1 and LATS2 and Non-Cardia Gastric Cancer
Authors: Ma Licong , Tian Xuyang , Gao Fang , Dong Wenjie , Dang Tong , Jia Yanbin 
Published: March 2020

Abstract
Background: Many studies have found that large tumor suppressor kinase 1 (LATS1) and LATS2 play important roles in many diseases, but studies have been rare on the relationship between these genes and non-cardia gastric cancer (GC). We performed a case-control association study to investigate the associations between single nucleotide polymorphisms (SNPs) in LATS1 and LATS2 genes and Helicobacter pylori (H. pylori) infection as well as the risk of non-cardia GC. Methods: First, H. pylori infection was determined by the serological test using enzyme-linked immunoassay. Then genotyping of SNPs was performed for 808 samples by the Taqman method. Finally, unconditional logistic regression was used to calculate the odds ratios (ORs) and 95% confidence intervals (CIs), adjusted for age and gender, for the association of each SNP with the infection of H. pylori, the risk of non-cardia gastric cancer, as well as the expression of LATS1 and LATS2 proteins in non-cardia GC tissues, using the codominant, dominant, recessive, overdominant, and additive inheritance models, respectively. Results: The statistical results showed that LATS2 rs9552315 was associated with H. pylori infection, and the CC+CT genotype could reduce the risk of H. pylori infection (odds ratio [OR]: 0.549, 95% confidence interval [CI]: 0.339–0.881, P<0.05) compared with the TT genotype in a dominant model. LATS1 rs9393175 was associated with the risk of non-cardia GC, and the AG genotype reduced the risk of non-cardia GC (OR: 0.702, 95% CI: 0.516–0.952, P<0.05) compared with the GG+AA genotype in an overdominant model. LATS2 rs9509492 was associated with the risk of GC in an additive model. No associations were found between five SNPs and expression of LATS1 and LATS2 proteins in non-cardia GC tissue. Conclusions: LATS2 rs9552315 CT genotype may be a protective factor against infection of H. pylori. LATS1 rs9393175 AG genotype and LATS2 rs9509492 GG genotype may be protective factors for non-cardia GC.
Epidemiology and clinico-pathological characteristics of current goat pox outbreak in North Vietnam
Authors: Hong Trang Pham , Lila Mohd Azmi Mohd , Rahaman Nor Yasmin Abd. , Lan Huong Lai Thi , Thi Lan Nguyen , Van Khien Do , Noordin Mohamed Mustapha 
Published: March 2020

Abstract
Background: In view of the current swine fever outbreak and the government aspiration to increase the goat population, a need arises to control and prevent outbreaks of goat pox. Despite North Vietnam facing sporadic cases of goat pox, this most recent outbreak had the highest recorded morbidity, mortality and case fatality rate. Thus, owing to the likelihood of a widespread recurrence of goat pox infection, an analysis of that outbreak was done based on selected signalment, management and disease pattern (signs and pathology) parameters. This includes examination of animals, inspection of facilities, tissue sampling and analysis for confirmation of goatpox along with questionaires.

Results: It was found that the susceptible age group were between 3-6 months old kids while higher infection rate occurred in those under the free-range rearing system. The clinical signs of pyrexia, anorexia, nasal discharges and lesions of pocks were not restricted to the skin but have extended into the lung and intestine. The pathogen had been confirmed in positive cases via PCR as goat pox with prevalence of 79.69%.

Conclusions: The epidemiology of the current goat pox outbreak in North Vietnam denotes a significant prevalence which may affect the industry. This signals the importance of identifying the salient clinical signs and post mortem lesions of goat pox at the field level in order to achieve an effective control of the disease.
Higher levels of dynamin-related protein 1 are associated with reduced radiation sensitivity of glioblastoma cells
Authors: Shen Chiung-Chyi , Cheng Wen-Yu , Chow Kuan-Chih , Chiao Ming-Tsang , Yang Yi-Chi 
Published: March 2020

Abstract
Background : Dynamin-related protein 1 (DRP1) is a GTPase involved in mitochondrial fission, mitochondrial protein import, and drug sensitivity, suggesting an association with cancer progression. This study was conducted to evaluate the prognostic significance of DRP1 in glioblastoma multiforme (GBM). Methods : DRP1 expression was measured by immunohistochemistry and Western blotting. Correlations between DRP1 expression and clinicopathological parameters were determined by statistical analysis. Differences in survival were compared using the log-rank test. Results : DRP1 expression was detected in 87.2% (41/47) of the investigated patients with GBM. The patients with higher DRP1 levels had worse survival ( p = 0.0398). In vitro , the silencing of DRP1 reduced cell proliferation, invasive potential, and radiation resistance. The addition of shikonin inhibited DRP1 expression and increased drug uptake. Moreover, shikonin reduced the nuclear entry of DNA repair-associated enzymes and increased radiation sensitivity, suggesting that reducing DRP1 expression could inhibit DNA repair and increase the radiation sensitivity of GBM cells. Conclusions : Our results indicate that DRP1 overexpression is a prospective radio-resistant phenotype in GBM. Therefore, DRP1 could be a potential target for improving the effectiveness of radiation therapy.
Identification of favorable SNP alleles related to fruit traits in diverse apple germplasm
Authors: Han A Reum , Kim Seon-Ae , Kim Gi Hoon , Choi Cheol 
Published: March 2020

Abstract
Background: The Apple ( Malus × domestica Borkh.) is a valuable fruit crop worldwide, and receives considerable attention as one of the model plants of the Rosaceae family. The primary purpose of apple breeding programs is to generate novel apple cultivars with fruit quality traits that have high commercial value. To advance fruit-tree breeding systems, it is necessary to investigate the association between genomes and targeted traits. Genome-wide association studies (GWAS) are promising to analyze the associations between the genome and traits in fruit tree crops. Results: In this study, we evaluated 10 major fruit quality traits including titratable acidity (TA), soluble solids content (SSC), and skin color from 301 apple germplasms for four years (2015-2018). GWAS analysis was performed using SNP data generated via genotyping by sequencing (GBS) and identified SNPs significantly related to the fruit quality traits. For TA, significant association loci were detected on chromosome 16 and genes in the candidate regions related to malate transporter. The GWAS loci of SSC was found on chromosome 15, where genes related to sucrose synthase and transporter are located. Significant SNPs associated with fruit skin color were identified on genetic region near the MYB1 gene on chromosome 9, which regulates anthocyanin biosynthesis. SNPs identified by the GWAS and Linkage disequilibrium (LD) analysis were further confirmed with high resolution melting (HRM) analysis to discover specific polymorphisms in the melting curve. Conclusion: Overall, these results could identify several candidate genes and SNP markers associated with the fruit quality traits, thus validation of these SNPs enabled marker-assisted selection (MAS). The candidate SNPs and genes observed in this study will contribute to a better understanding of genetic basis for the important fruit quality traits and provide tools for generation of novel cultivars with the quality traits for advancement of apple industry.
iTRAQ-based quantitative proteomics analysis of cantaloupe (Cucumis melo var. saccharinus) after cold storage
Authors: Song Wen , Tang Fengxian , Cai Wenchao , Zhang Qin , Zhou Fake , Ning Ming , Tian Huan , Shan Chunhui 
Published: March 2020

Abstract
Background: During the low temperature storage, cantaloupe is susceptible to the cold stress, resulting in the loss of edible and commercial quality. To ascertain the molecular mechanisms of cold tolerance in cantaloupe, cold-sensitive cultivar Goldqueen-308 (GE) and cold-tolerant cultivar Jiashi-310 (JS) were used for quantitative proteomic analysis with iTRAQ in parallel. Results: In this work, two commercial cultivars were treated at 0.5℃ for 0, 12 and 24 days. Phenotypes assays showed that GE suffered a more severe damage as the cold treatment time extended. Proteomic analysis revealed that the number of differentially expressed proteins (DEPs) changed significantly over time in cold-exposed cantaloupe. Comparing with GE, JS responded in a prompter manner in terms of expressing cold-responding proteins during the similarly cold treatment. Furthermore, much more different groups of proteins were mobilized in response to the cold treatment in JS comparing with GE. Metabolic analysis indicated that more amino acids were up-regulated in JS during the early phases of cold stress. This study also identified some DEPs since they were up-regulated in JS or down-regulated in GE in terms of molecular mechanisms, which were mainly related to carbohydrate and energy metabolism, structure proteins, ROS scavening, amino acid metabolic and signaling transduction. Moreover, iTRAQ analysis was confirmed to be reliable via the results of phenotypes assays, metabolic analysis and q-PCR validation. Conclusion: By proteomics information,we found that the prompt response and the significant mobilization of proteins in JS maintained a higher level of cold tolerance, and the delay of cold response in GE could be a critical reason for the severe chilling injury. The candidate proteins we found will be the basis of future studies for further investigations and our findings may help to better understand the novel mechanisms of cold tolerance in cantaloupe.