bluuebunny/crossref_metadata_2025_split huggingface dataset:
The universal sample holders of microanalytical instruments of FIB, TEM, NanoSIMS, and STXM-NEXAFS for the coordinated analysis of extraterrestrial materials
Authors: Ito Motoo , Tomioka Naotaka , Uesugi Kentaro , Uesugi Masayuki , Kodama Yu , Sakurai Ikuya , Okada Ikuo , Ohigashi Takuji , Yuzawa Hayato , Yamaguchi Akira , Imae Naoya , Karouji Yuzuru , Shirai Naoki , Yada Toru , Abe Masanao 
Published: June 2020

Abstract
We developed universal sample holders (the Kochi grid, Kochi clamp, and Okazaki cell) and a transfer vessel (facility-to-facility transfer container (FFTC)) to analyze sensitive and fragile samples, such as extremely small extraterrestrial materials. The holders and container prevent degradation, contamination due to terrestrial atmosphere (water vapor and oxygen gas) and small particles, as well as mechanical sample damages. The FFTC can isolate the samples from the effects of the atmosphere for more than a week. The Kochi grid and clamp were made for a coordinate micro/nano-analysis that utilize a focused-ion beam apparatus, transmission electron microscope, and nanoscale secondary ion mass spectrometry. The Okazaki cell was made as an additional attachment for a scanning transmission X-ray microscope that uses near edge X-ray absorption fine structure. The coordinated analysis involving these holders was successfully carried out without any sample damage or loss, thereby enabling us to obtain sufficient quality of analytical datasets of textures, crystallography, elemental/isotopic abundances, and molecular functional groups for µm-sized minerals and organics in both an Antarctic micrometeorite and a carbonaceous chondrite. We will apply the coordinated analysis to acquire the complex characteristics in samples that obtain by the future spacecraft sample return mission.
Identification of LncRNA Prognostic Biomarkers Associated with Copy Number Variants in Gastric Cancer
Authors: Wang Anqiang , Chen Jiahui , Zhou Kai , He Qifei , Ji Ke , Ji Xin , Wu Xiaojiang , Zhang Ji , Bu Zhaode , Ji Jiafu 
Published: June 2020

Abstract
Backgrounds: Gastric cancer is one of the most common gastrointestinal carcinomas worldwide, with a poor prognosis. Prognosis prediction is very important in the treatment of gastric cancer .This study aimed to explore the prognostic value of lncRNA deregulated by copy number variants (CNVs) in gastric cancer. Methods: Multi-omics cluster analysis was performed to identify subtypes on the prognosis associated coding genes, CNVs and methylation sites. We conducted survival analyses on median expression level for all identified lncRNAs. Finally, we constructed and validated a prognostic model on lncRNAs with data of public and our center. Results: As a result, we identified six subtypes of gastric cancer with different prognosis (P=0.00446) and a total of 83 disease associated lncRNAs. We finally obtained five prognostic lncRNA biomarkers. Survival analyses showed that the high expression of all identified lncRNAs positive associated with worse prognosis. We also found that the prognostic model on five identified lncRNAs could predict survival with high 5-years AUC 0.69. And the differences of survival between high risk and low risk groups were significant in both of our and public database. In the multivariable analyses, we found that the prognostic model was an independent prognostic factor (p = 0.0104).Conclusions: We concluded that the prognostic model on five identified lncRNAs was closely related to the overall survival of gastric cancer and may serve as promising prognostic biomarkers of gastric cancer.
A Practical Framework for Conducting Video Telemedicine Visits with Trainees
Authors: Kwan Brian , Charat Stacy , Wooten Darcy , Hill Deanna , Restaino Nivedita , Friedman Lawrence 
Published: June 2020

Abstract
Background:Due to COVID-19, traditional clinical education for senior medical students has largely halted. In response to social distancing, many outpatient practices have transitioned to Telemedicine (TM). While TM has been integrated into undergraduate medical education curricula at many institutions, a published roadmap for incorporating learners into TM is not readily available.Aim:To describe one healthcare system’s transition to TM and propose a framework for including learners in video TM visitsSetting:Primary care clinics at an academic medical centerParticipants:Medical students, facultyProgram Description:Training protocols were distributed to providers and students. Multi-provider video visits were enabled for distance teaching. Students tested potential workflow models and provided feedback, facilitating creation of a 4-phase construct to guide inclusion of learners in video TM encounters.Program Evaluation:We employed a rapid feedback cycle to improve workflow process and to modify trainee and preceptor instructions. We analyzed student comments for narrative themes to plan for future evaluation of video TM encounters.Discussion:TM will be increasingly used in the provision of medical care. Clinician-educators will need to innovate in order to meet patient and learner expectations. TM will be an integral teaching tool and may enhance the educational experience.
Predictive Significance of Arachidonate 15-Lipoxygenase for Eosinophilic Chronic Rhinosinusitis with Nasal Polyps
Authors: Liang Zhuoping , Yan Bing , Liu Chang , Tan Ruyu , Wang Chengshuo , Zhang Luo 
Published: June 2020

Abstract
Background Eosinophilic chronic rhinosinusitis with nasal polyps (ECRSwNP) exhibits a poorer outcome compared with non-eosinophilic chronic rhinosinusitis with nasal polyps (nonECRSwNP), so it is significant to identify effective markers to differentiate ECRSwNP in guiding the treatment strategies of these patients. Although arachidonate 15-lipoxygenase (ALOX15) is positioned as a marker of eosinophilic inflammation, its study in differentiating ECRSwNP has not been reported. The aim of this study is to assess the potential of ALOX15 in distinguishing and predicting ECRSwNP. Methods Forty-eight patients with chronic rhinosinusitis with nasal polyps (CRSwNP), including 30 ECRSwNP and 18 nonECRSwNP patients, were enrolled. ALOX15 mRNA level was determined in polyps by real-time polymerase chain reaction (RT-PCR). The patients’ baseline characteristics were evaluated and analyzed for correlations with ALOX15. Receiver operating characteristic (ROC) curve was used to assess the predictive significance of the potential predictors for ECRSwNP. Results ALOX15 mRNA level was significantly higher in ECRSwNP patients than in nonECRSwNP patients ( P < 0.001). ALOX15 mRNA was significantly correlated with tissue and blood eosinophil percentages ( r = 0.565, P < 0.001 and r = 0.395, P = 0.006), olfaction scores ( r = 0.400, P = 0.005), total visual analogue scale (VAS) symptom scores ( r = 0.383, P = 0.007), ethmoid/maxillary sinus (E/M) ratio ( r = 0.463, P = 0.001), and endoscopy scores ( r = 0.409, P = 0.004). Logistic regression analysis showed ALOX15 mRNA level and percentage of blood eosinophils to be predictive factors for ECRSwNP ( P = 0.004 and P = 0.036, respectively). ROC curve indicated ALOX15 to have high predictive accuracy for ECRSwNP (area under the curve (AUC) = 0.909), which was further improved by combination of ALOX15 with percentage of blood eosinophils (AUC = 0.933). Conclusions The relative ALOX15 mRNA level alone or in combination with blood eosinophils might be a reliable biomarker for predicting a diagnosis of ECRSwNP.
Severe Fatigue Due to Valproate-induced Hypothyroidism in a Case of Bipolar Disorder
Authors: Kanamori Tadashi , Suzuki Masahiro , Kaneko Yoshiyuki , Yamada Kouju , Kubo Hideyuki , Uchiyama Makoto 
Published: June 2020

Abstract
Background: Valproate-induced hypothyroidism is a rare conditionand has been considered asymptomatic. Here, we report a case of bipolar I disorder who developed symptomatic valproate-induced hypothyroidism.Case presentation: A 44-year-old woman with bipolar I disorder complained of severe fatigue after starting valproate. She showed a hormonal pattern of central hypothyroidism. Thyroid autoantibodies were negative, and no pituitary abnormality was seen on magnetic resonance imaging. After stopping valproate, her severe fatigue rapidly improved with normalizing thyroid function.Conclusions:Our case suggests that valproate-induced hypothyroidism should be considered when patients complain of excessive fatigue under treatment with valproate.
Thevebioside, the Active Ingredient of Traditional Chinese Medicine, Inhibits SRC3 Mediated Signaling by Ubiquitination to Induce NSCLC Cells Apoptosis
Authors: Yao Chao , Su Lin , Zhang Fei , Zhu Xiaowen , Zhu Yangzhuangzhuang , Wei Luyao , Jiao Xiaoning , Hou Yifei , Chen Xiao , Wang Wantao , Wang Jie , Zhu Xiandan , Zou Chunpu , Zhu Shiguo , Xu Zihang 
Published: June 2020

Abstract
Background:The high incidence and mortality of lung cancer has been a widespread concern in the world, among which non-small cell lung cancer (NSCLC)accounts for more than 85% of lung cancer, and its 5-year prognosis is pretty poor. Accumulating studies have shown that traditional Chinese medicine (TCM) and its active ingredients have shown good anti-tumor activity. Based on the results of our previous high-throughput screening, it was the first time to find that Thevebioside (THB, an active ingredient from TCM) is sensitive to NSCLC cells, and then we will still the first to reveal the underling mechanism of its tumor suppressive activity.Methods: In this study, MTT and colony formation assay were used to detect cell growth. Annexin V/ propidium iodide, Hoechst 33342 were applied to test the process of cellular apoptosis or cell cycle arrest which determined by flow cytometry in NSCLC (A549 and H460) cells. qRT-PCR and Western blot was utilized to value the level of expression of SRC&(SRC1-3) and apoptosis related proteins. The ubiquitination degradation of SRC3 was through proteasome dependent pathway were examined by the usage of CHX and MG132 and the co-immunoprecipitation assay. The anticancer activity of THB, Neritaloside，Odoroside H and digoxin in vivo were monitored in nude mice by subcutaneous inoculation or intrapulmonary injection of A549 cells and then administrated these agents (2 mg/ kg or 4mg/kg) by intraperitoneal injection three times a week for 30 days. Tumor growth was monitored by a luciferase living imaging system. Body weight of mice and tumor volume were measured weekly. The survival carve was recorded. Pathological histology of liver, kidney and heart were detected by H&E staining, and its functions were tested by ELISA. Results：THB effectively inhibits the outgrowth of NSCLC cells (A549 and H460) by inducing cellular apoptosis. SRC-3 was significantly down-regulated after THB treatment dependent on ubiquitin-proteasome-mediated degradation, which subsequently inhibited the AKT signaling pathway to promte apoptosis. Animal study supported in vitro results, showing THB had a good suppressive effect on tumor growth. Besides, it also indicated that THB without overt cytotoxic effect on liver, kidney and heart.Conclusion：THB , as an active extract from TCM，exerted inhibitory effect on tumor growth of NSCLC by down-regulating the expression of SCR-3 rely on the ubiquitin-proteasome degradation pathway, thereby inhibiting the AKT signaling to induce cellular apoptosis , with minimal toxicity.
Case Report: Diagnosis and Treatment of a Giant Retroperitoneal Liposarcoma Presenting as an Irreducible Inguinal Hernia
Authors: Yuan Zhiqing , Li Qiwei , Sun Jianhua , Zhou Wei , Chen Tao 
Published: June 2020

Abstract
Background: Retroperitoneal liposarcoma protrude to the inguinoscrotal area presenting as an irreducible inguinal hernia is extremely rare. For the rare cases and little experience of diagnosis and treatment of this disorder, the clinical guidelines are vacant. We report a successful example for the management of a giant retroperitoneal liposarcoma extending to the inguinoscrotal area. Case presentation: A 55-year-old male patient was admitted to our hospital in August 2018 with a large left inguinal mass without abdominal pain or digestive symptoms. Preoperative contrast-enhanced computed tomography revealed an abdominopelvic huge mass, and ultrasound guided biopsies showed liposarcoma. The patient also suffered from dilated cardiomyopathy and the left ventricular ejection fraction is only 39%. The left renal pedicle was squeezed by the mass and the left glomerular filtration rate is as low as 29.25ml/min. Intraoperatively, the mass was incarcerated in the inguinal canal and involved the left testis. We performed a radical tumor resection with two incisions, including resection of the retroperitoneal tumor, resection of the scrotal tumor and a tension-free repair of left inguinal hernia. The resected specimen for the retroperitoneal part measured 50*28*9 cm, weighed 13.5 kilograms and the scrotal part measured 16.5*7*4.5 cm, weighed 6.2 kilograms. Pathologically, the tumor was diagnosed as a well-differentiated liposarcoma, and originated from perirenal fat. The patient did not undergo adjuvant therapy post-operation and is completely clinical remission fifteen months after the operation. Conclusions: Careful distinction for inguinoscrotal mass is essential to minimize complications and improve patient prognosis. The prime principle to treat well differential retroperitoneal liposarcomas is radical resection with protection of vital organs and vessels.
Impact of Neutrophil-Lymphocyte Ratio on Long-term Outcomes of Patients with Coronary Chronic Total Occlusion After Percutaneous Coronary Intervention
Authors: Gao Ke , Li Bo-Lin , Yang Lei , Xue Yan-Bo , Yang Wan-Ying , Wu Yue , Zheng Xiao-pu 
Published: June 2020

Abstract
Background: The impact of preprocedural neutrophil-lymphocyte ratio (NLR) level on subsequent adverse cardiovascular events and health status in coronary chronic total occlusion (CTO) patients after stent implantation is unclear. The present study aims to evaluate the impact of NLR level on long-term clinical outcomes and health status of CTO patients after percutaneous coronary intervention (PCI) in the drug eluting stent era.Methods: A total of 311 CTO patients who underwent successful PCI with new-generation drug-eluting stent and with follow-up were enrolled. Patients were classified into 3 groups according to the tertiles of NLR level at baseline. The primary endpoint (major adverse cardiac events, MACE) was a composite of cardiac death, target-vessel myocardial infarction (MI), and ischemia-driven target-vessel revascularization (TVR) on follow-up. Multivariable COX regression analysis was performed to estimate the relationships between the parameters and MACE.Results: Compared with the low and intermediate tertile groups, preprocedural platelet-lymphocyte ratio (PLR) level and age were significantly higher in the high NLR tertile group. After a median follow-up of 32 (interquartile range: 20 to 44) months, MACE was observed in 10 patients (9.7%) in the low tertile, in 21 (20.2%) in the intermediate tertile, and in 31 (29.8%) in the high tertile group (P = 0.001). Kaplan-Meier analysis demonstrated a significantly higher incidence of MACE, mainly driven by TVR, in patients with high tertile than the low and intermediate tertile groups. Multivariable COX regression analysis showed NLR and occluded length as independent predictors of MACE. No statistically differences were observed regarding the five domains of Seattle Angina Questionnaire (SAQ) scores among the 3 groups (all P > 0.05).Conclusions: In conclusion, in patients who underwent PCI for CTO lesions, elevated NLR level was independently associated with MACE (driven by TVR) on follow-up.
Tobacco rattle virus -induced gene silencing in Hevea brasiliensis
Authors: Li Hui-Liang , Guo Dong , Wang Ying , Zhu Jia-Hong , Qu Long , Peng Shi-Qing 
Published: June 2020

Abstract
BackgroundSince it is very difficult to obtain gene knockouts in rubber tree (Hevea Brasiliensis) due to low genetic transformation efficiency. Virus-induced gene silencing (VIGS) is a powerful gene silencing tool that has been intensively applied in plant. Up to now, the application of VIGS in rubber tree has not yet been reported.ResultsHevea brasiliensis phytoene desaturase (HbPDS) was identified in H. brasiliensis genome. The prediction of small interfering RNAs (siRNAs) from HbPDS and the silencing gene fragment (SGF) were predicted and a length of 409 bp SGF was chosen to be tested. We show that the tobacco rattle virus (TRV) -VIGS is able to induce effective HbPDS silencing in rubber tree. The TRV-VIGS system has the potential for functional gene studies in rubber tree.ConclusionsThis is the first time to report VIGS in rubber tree. The present TRV-VIGS method could be further applied to produce gene silenced rubber tree plants, to advance functional gene of rubber tree. The applied TRV-VIGS method will achieve deeper underground into the natural rubber biosynthesis and regulation in this important rubber-producing plant.
A Real-World Study for Polymyxin B in the Treatment of Carbapenems Resistant Gram-Negative Bacilli
Authors: Zhang Xiaojuan , Qi Shaoyan , Duan Xiaoguang , Han Bing , Zhang Shuguang , Liu Shaohua , Wang Haixu , Zhang Haibo , Sun Tongwen 
Published: June 2020

Abstract
Background: High morbidity and mortality due to carbapenem-resistant Gram-negative bacilli (CRGNB) was a challenge for clinicians has led to the resurgence of polymyxin B (PMB) use in the last decade. The goal of our multicenter, real-world study was to evaluate the efficacy and safety of PMB in the treatment of CRGNB.Methods: The real-world study included the patients with intravenous PMB at least 7 days during the period of October 2018 to June 2019. Data was collected from electronic patients register and follow-up. The primary outcome was 28-day mortality, the secondary outcomes included hospital mortality, occurrence of adverse events during PMB therapy. Associations between these variables and 28-day mortality or all-cause hospital mortality were explored through univariate analyses and multivariable logistic regression. At the same time, therapeutic effects were observed. Results: The study included 100 patients. There were 39% presence of septic shock, 49% need mechanical ventilation at the beginning of therapy. The infection and condition improved after 7 days of PMB treatment. The major adverse reactions occurred in 16 cases (16%). The overall 28-day mortality was 40%. In terms of clinical characteristics, mean Sequential Organ Failure Assessment (6.77 versus 9.25，P = 0.004)，mean Acute Physiology and Chronic Health Evaluation II (APACHEII) scores (16.17 versus 19.78, P = 0.016) and the number of patient with mechanical ventilation (21 versus 30, P = 0.000) or septic shock (17 versus 32, P = 0.000) were lower in survivors group than nonsurvivors group. The mortality of 85 patients with identify pathogens was 38.82%, while the mortality of patients with negative pathogen culture results was 46.67% (P = 0.580). Multivariate analysis showed that mechanical ventilation (P = 0.023, OR = 3.5; CI: 1.194–10.739), septic shock (P = 0.002, OR = 5.960; CI: 1.923–18.473) were associated with 28-day mortality.Conclusion: Our research found that PMB may be as effective and safe as standard antibiotics for the treatment of CRGNB. Timely and appropriate use of PMB will have a positive impact on the clinical outcomes of patients with sepsis in CRGNB.
Risk of Anterior Cruciate Ligament Injury in Obese Population: A Retrospective Study in a Single Center in Saudi Arabia
Authors: Alsayed Hasan , Alkhateeb Mohammed , Aldossary Asma Abdulaziz , Houbani Khalid , AlJamaan Yousef , Aldakheel Dakheel , Alrashidi Yousef 
Published: June 2020

Abstract
Background: The Anterior cruciate ligament (ACL) injury is among the most common orthopedic injuries(1). The Elevated Body Mass Index (BMI), can contribute to non-contact ACL injury(5). This study aims to assess the risk of (ACL) injury in obese people (BMI) more than 30 Kg\m2).Methodology: This is a retrospective cohort series was done in a tertiary care center in Kingdom of Saudi Arabia . A total of 302 patients, who had ACL reconstruction surgery in a period between (January 2008 to December 2018) were included. (BMI) was classified based on WHO classification, baseline characteristics data include: Age, gender, nationality, type of injury, classification of injury, mechanism of injury, and mode of injury. Binary regression analysis was done to predict the effect of obesity on a selected baseline characteristic of patients (type of sport, Isolated\ combined, type of injury, reinjury rate).Result:Our study showed that sport related injury is significantly higher among overweight and obese group (P: 0,007). Moreover, the combined ACL tear was higher among overweight and obese group (P: 0,001). In binary regression analysis for the selected baseline characteristics we found that obese people have chance to develop combined (ACL) injury 2 times higher when compared to those with isolated ACL injury with a p value of (0,003). However, the other selected variables like mode of injury, type of sport, mechanism of injury and type of graft were not significance. Conclusion: Elevated BMI was associated with higher risk of developing ACL tear.
Postoperative incidence of seizure and ischemic events in patients with epileptic type moyamoya disease: a meta-analysis of single rate
Authors: LIU JINGJING , Xu Qinlan , Niu Hongchuan , Wang Rong , Ye Xun , Liu Xianzeng 
Published: June 2020

Abstract
BackgroundSurgery is a conventional mature treatment for moyamoya disease (MMD). However, whether surgery is also an effective therapy for epileptic type MMD has seldom been investigated systematically. The study aims to summarize the pooled postoperative incidence of seizure and ischemic events in patients with epileptic type moyamoya disease.MethodThe study was a systematic review and critical appraisal with a meta-analysis of cohort studies, both prospective and retrospective. Studies were identified by a computerized search of Pubmed, EMBASE, Web of Science, Wanfang, and CNKI databases. In a literature search, a total of 8 cohort studies were identified. The I2statistic was used to quantify heterogeneity. A fixed-effect model was used to synthesize the results. The linear regression test of funnel plot asymmetry was used to estimate the potential publication bias.ResultsThe pooled estimated postoperative incidence of seizure in patients with epileptic type moyamoya disease was 21.52%. The pooled estimated postoperative incidence of ischemic events in patients with epileptic type moyamoya disease was7.42%. Low substantial heterogeneity and potential publication bias were present.ConclusionEvidence from this study suggests that the postoperative incidence of seizure and ischemic events is relatively low. Surgery is an effective and secure therapy for patients with epileptic type moyamoya disease.