bluuebunny/crossref_metadata_2025_split huggingface dataset:
LINC00152 promotes Ovarian tumor progression and Predicts Poor Prognosis by inhibiting the ubiquitination of BCL6
Authors: Wang Shunni , Weng Weiwei , Chen Tingting , Xu Midie , Wei Ping , Li Jing , Lu Linghui , Wang Yiqin 
Published: April 2020

Abstract
The authors have withdrawn this preprint due to author disagreement.
Investigating the relationship between theory of planned behavior construct with the pregnant women intention about neonatal care
Authors: Navabi Azamalsadat , shamsi mohsen , khorsandi mahboobeh , zamanian maryam 
Published: April 2020

Abstract
Background: Considering that neonatal are the most sensitive group to kind of diseases, the present study aimed an investigating the relationship between theory of planned behavior construct with the pregnant women intention about neonatal care.Method: In this cross-sectional study, 100 pregnant women in trimester of pregnancy were selected by random sampling. Data was collected using valid and reliable questionnaire and interviews conducted with pregnant women. The questionnaire included demographic characteristic of the mothers and theory planned behavior construct. Finally the data were analyzed by SPSS 20 T-test, Chi-square, Correlation, ANOVA and regression.Results: The average age of the sample was 26.12±4.9 and the average duration of marriage age was 3.9 ±2.74 years. There was a high correlation between perceived behavioral control and behavioral intention (r=0.40; p < 0.001). Over 40% of pregnant women were shown to lack overall Knowledge about the items related to neonatal care. The mothers who were better prepared for parenting tended to have a higher level of schooling, perceived control behavior and knowledge. Regression analysis showed that the knowledge and perceived behavior control R2 =32% in the prediction of neonatal care intentions.Conclusions: Findings of this study show support for the predictive ability of the theory of planned behavior in predicting for neonatal care therefore the design of educational intervention should be based on intention and knowledge as the most important predictors of maternal behavior.
The influence of laparoscopy-assisted gastrectomy versus open gastrectomy to long-term survival for early gastric cancer: an updated meta-analysis based on randomized controlled trials
Authors: Zhai Zhao , Lou Shenghan , Zhu Ziyu , Cong Xiliang , Gao Jialiang , Han Bangling , Yin Xin , Zhang Yu , Xue Yingwei 
Published: April 2020

Abstract
Background There is a controversy for the therapeutic effect of laparoscopy-assisted gastrectomy (LADG) and open gastrectomy for a long time. The meta-analysis in this article evaluates and compares the effectiveness of these two treatments to patients' long-term survival. Method We searched MEDLINE (PubMed), EMBASE (Ovid) and the Cochrane Library and identified all qualified researches from January 2002 to January 2020. The statistic analysis is performed by Revman 5.3 and Version 3 Comprehensive Meta-analysis. Through the results, we compared the advantages and long-term survival of LADG and ODG. Result According to the result of 8 randomized controlled trials which involves 2650 patients with early gastric cancer, compared with ODG, LAGD has longer surgery time (MD=78.11,95%CI=59.14-97.07,P<0.00001), less blood loss (MD=-100.59,95%CI=-127.71--73.46,P<0.00001), a shorter incision (MD=-12.25,95%CI=-13.18--11.31,P<0.00001), shorter time to start the first time of flatus (MD=-0.66,95%CI=-1.01--0.32,P=0.0001), shorter hospital stay (MD=-0.94,95%CI=-1.66--0.22,P=0.01), and lower frequency of analgesic utilization (MD=-1.69,95%CI=-2.18--1.21,P<0.00001). However, there is no statistical difference between ODG and LAGD on the number of harvested lymph nodes, time of the first meal, short-term surgical complications, cancer recurrence rate, and long-term survival rate. Conclusion According to the result of meta-analysis, compared with ODG, LADG has a significant advantage in blood loss in surgery, early rehabilitation, scar development, management of postoperative pain, etc. Meanwhile, LADG and ODG have the same performance on long-term survival. Therefore, we believe LADG is a safe and effective replacement of ODG for early gastric cancer.
High- power radiofrequency ablation guided by ablation index for pulmonary vein isolation
Authors: Zhu Xuefeng , Wang Chunxiao , Li Jianping , Li Wenjing , Chu Hongxia , Zhou Huihui 
Published: April 2020

Abstract
Background

Proposed to facilitate pulmonary vein isolation (PVI), high-power ablation may cause extracardiac damage. This study evaluated the safety and efficacy of ablation index (AI) guided high-power ablation first in an animal model and subsequently in a clinical study.
Methods

Outcomes of radiofrequency (RF) applications were compared in a swine ventricular endocardial model (n = 10 each for 50W, 40W and 30W; AI = 500), and in 100 consecutive patients with paroxysmal atrial fibrillation undergoing PVI (40W [last n = 50] vs. 30 W [first n = 50]; target AI = 400/500 on posterior/anterior wall, respectively). Acute PV reconnection was assessed post adenosine administration 20 minutes after ablation.
Results

In swine ventricular endocardial RF applications, use of 50W and 40W vs. 30W was associated with greater tissue lesion depth (5.06 ± 0.16 and 4.38 ± 0.13 mm vs. 3.95 ± 0.16 mm; P < 0.001) and smaller lesion maximum diameter (7.81 ± 0.15 and 8.42 ± 0.18 mm vs. 9.08 ± 0.15 mm; P < 0.001). Tissue necrosis caused by 50W vs. 40W and 30W was the deepest and largest (3.15 ± 0.18 mm vs. 2.71 ± 0.17 and 2.42 ± 0.13 mm; and 5.58 ± 0.18 mm vs. 5.18 ± 0.16 and 3.94 ± 0.17 mm; respectively; P < 0.001). In PVI, use of 40W vs. 30W was associated with shorter procedure time (56.54 ± 1.81 min vs. 76.55 ± 2.34 min; p < 0.001) and ablation time (35.85 ± 14.87 min vs. 51.01 ± 17.99 min; p < 0.001); lower RF energy per point (909.02 ± 354.57J vs. 1045 ± 376.60J; p < 0.001); higher first-pass PVI (87% vs. 72%; P < 0.01); lower acute PV reconnection (22% vs. 41%; P < 0.01); no complications in either group; and similar sinus rhythm maintenance at 12 months (92% vs. 84%; P = 0.22).
Conclusions

AI-guided high-power (40W) vs. conventional (30W) PVI was related to a reduced time for procedure and was considered safe, with diminished acute pulmonary vein reconnection.
Side Effects of Azathioprine in Patients with Ocular Behçet’s disease
Authors: Masoumi Maryam , Davatchi Fereydoun , Shahram Farhad , Akhlaghi Maassoumeh , Faezi Seyedeh Tahereh , Kavosi Hoda , Shadmanfar Soraya , Alikhani Majid , Moradi Soroush 
Published: April 2020

Abstract
Background:

Behçet’s disease is an autoimmune, rare, multi-system vasculitis characterized by recurrent oral and genital ulcers, uveitis, skin lesions, and arthritis. Azathioprine has been well established as an effective therapy among other immunosuppressive drugs; however, concerns remain about the safety of azathioprine. This study is conducted to determine various types and prevalence of adverse effects of azathioprine in patients with ocular Behçet’s.
Methods:

We carried out a cross-sectional study on 165 patients with confirmed diagnosis of Behçet’s who suffered from ocular involvement. Data concerning the different episodes of the disease, including severity, recurrence, relapse, recovery and flare up of Behçet’s disease, were collected retrospectively, as well as the azathioprine dosage and duration of consumption.
Results:

Of 165 enrolled patients, 103 patients (62.43%) were male and 62 patients (37.57%) were female. Adverse events of treatment with azathioprine occurred in 11 patients (6.67%). Side effects occurred in 11 patients (6.67%) taking azathioprine. These side effects included elevation of liver enzyme in 4 patients (2.43%), hypersensitivity reaction in 2 patients (1.21%), leukopenia in 2 patients (1.21%), nausea in 1 patient (0.61%), skin tumor in 1 patient (0.61%) and concomitant pancreatitis and hepatitis in 1 patient (0.61%). Six of these cases discontinued and five of them tapered the dose. In addition, we observed severe leukopenia in two patients.
Conclusion:

Administration of azathioprine, with due attention to the signs, symptoms, and severity of the disease, results in a reduced rate of adverse effects, and resolving of the ocular involvement in Behçet’s disease patients.
The function of CISH in T cell differentiation and proliferation
Authors: Hu Zhangyong , Zhou Yan , Li Tiane , Zhang Lei , Zhou Min , Yang Jun , Wang Shuang , Yang Xuemei , Li Yanfang 
Published: April 2020

Abstract
Background: Previous studies have shown that cytokine-inducible SH2 containing protein (CISH) gene polymorphism is related to the differences in the outcomes of hepatitis B virus (HBV) infection. T cells play an important role in HBV infection. We tested the hypothesis that the CISH affects the outcome of HBV infection by influencing the T cells.Methods: CISH gene and shRNA were cloned into pCDH and pLKO.1 plasmid, respectively. We used 293T cells to package the lentivirus and collected the lentiviral supernatant to infect CD3 T cells and compared the differences in T cell proliferation, differentiation, T-cell receptor Vβ (TCRVβ) subfamily expression, and gene expression in each group. Results: We found that CISH had a negative effect on T cell proliferation. The expression of TCRVβ was also affected. The expression of TRVβ3.1 and TRVβ12 was up-regulated by CISH. Reducing CISH expression contributed to the formation of CD8+T cells. Gene ontology (GO) analysis results showed that CISH mainly regulated chromosome segregation, nuclear division, and organelle fission in the biological process (BP) group; chromosomal region, centrosome, and spindle in cellular components (CC) group; ATPase activity, and carbohydrate binding in molecular function (MF) group. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis results showed that CISH primarily affected the p53 signaling pathway, PI3K-Akt signaling pathway and others. Conclusion: We found that CISH plays an important role in T cells and this helps to further clarify the pathogenesis of HBV infections.
Cervical curvature changes following laminectomy with lateral mass screw fixation: Does it relate with spinal cord shift and clinical efficacy
Authors: Hou Shu-bing , Sun Xian-ze , Liu Feng-yu , Gong Rui , Zhao Zheng-qi , Lu Kuan , Liu Yan-bing 
Published: April 2020

Abstract
Background

Laminectomy with lateral mass screw fixation (LCSF) is an effective operation type for the treatment of cervical spondylotic myelopathy (CSM), however, the cervical curvature loss is often observed in some patients after operation. Will the cervical curvature change affect the spinal cord drift distance and the decompression effect? The aim of this study is to investigate the effects of different cervical curvature on spinal cord drift distance and clinical efficacy.
Methods

A total of 78 cases of CSM patients underwent LCSF were included in this retrospective study. The cervical curvature was measured according to the Bordon method 6 months after the operation, and the patients were divided into two groups. Group A: 42 cases with reduced cervical curvature (0 < the cervical lordosis depth < 7 mm) and group B: 36 cases with normal cervical curvature (7 mm ≤ the cervical lordosis depth ≤ 17 mm). The spinal cord drift distance, laminectomy width, neurological functional recovery, axial symptom (AS) severity and the occurrence of C5 palsy in both groups were observed.
Results

The cervical lordosis depth was (5.1 ± 1.2) mm in group A and (12.3 ± 2.4) mm in group B (P < 0.05). The laminectomy width was (21.5 ± 2.6) mm in group A and (21.9 ± 2.8) mm in group B (P > 0.05). The spinal cord drift distance was (1.9 ± 0.4) mm in group A and (2.6 ± 0.7) mm in group B, with statistically significant difference between the two groups (P < 0.05). The postoperative JOA scores in both groups were significantly increased (P < 0.05), and there was no significant difference in the neurological recovery rate (61.5% vs 62.7%) between the two groups (P > 0.05). According to the grading standard of AS, the severity of AS in group A was significantly higher than that in group B (P < 0.05). Three cases (7.1%) of C5 palsy occurred in group A and 4 cases (11.1%) occurred in group B (P > 0.05).
Conclusion

After LCSF, more than half of the patients had cervical curvature loss. The smaller of the cervical curvature was, the shorter distance the spinal cord drifted backward. The loss of cervical curvature was related to the severity of axial symptoms, rather than the improvement of neurological function and C5 palsy.
Clinical features of imported cases of coronavirus disease 2019 in Tibetan patients in the Plateau area
Authors: lei Yu , huang xiaobo , bamu silang , lan yunping , lu jianli , zeng fan 
Published: April 2020

Abstract
Background: Coronavirus disease 2019 (COVID-19), caused by SARS-CoV-2, has rapidly spread throughout China, but the clinical characteristics of Tibetan patients living in the Qinghai-Tibetan plateau are unknown. We aimed to investigate the epidemiological, clinical, laboratory and radiological characteristics of these patients. Methods: We included 67 Tibetan patients with confirmed SARS-CoV-2 infection. The patients were divided into two groups based on the presence of clinical symptoms at admission, with 31 and 36 patients in the symptomatic and asymptomatic groups, respectively. The epidemiological, clinical, laboratory and radiological characteristics were extracted and analysed. Results: No patient had a history of exposure to COVID-19 patients from Wuhan or had travelled to Wuhan. The mean age of Tibetan patients was 39.3 years and 59% of the patients were male. Seven patients presented with fever on admission and lymphocytopenia was present in 20 patients. 47 patients had abnormal chest CTs at admission instead of stating that 20 were unchanged. Lactate dehydrogenase levels were increased in 31 patients. Seven patients progressed to severe COVID-19; however, after treatment, their condition was stable. No patients died. Of the 36 asymptomatic patients, the mean age was younger than the symptomatic group (34.4 ± 17.3vs 44.9 ± 18.1 years, P = 0.02). Lymphocyte count and prealbumin levels were higher in the asymptomatic group than the group with clinical symptoms (1.6 ± 0.5 vs 1.3 ± 0.6 and 241.8 ± 68.2 vs 191.9 ± 60.3, respectively; P < 0.05). Conclusions: Imported cases of COVID-19 in Tibetan patients were generally mild in this high-altitude area. Absence of fever or radiologic abnormalities on initial presentation were common.
Genome-wide association analysis uncovers candidate genes for forage quality traits in Brassica napus stems
Authors: wei lijuan , Liu Ruiying , Xu Wen , Zhu Yan , Ma Jinqi , Lin Ai , Li Jiana 
Published: April 2020

Abstract
Background

Brassica napus (rapeseed) is an important oilseed crop and its leaves and stems can also be used as animal feed. Lignocellulose content is closely related to the nutritional quality and palatability of animal feed. However, quantitative trait loci (QTLs) associated with acid detergent fiber (ADF) and neutral detergent fiber (NDF) contents in rapeseed stems have not yet been mapped.
Results

In this study, we used 494 B. napus accessions to perform genome-wide association studies (GWAS) of ADF and NDF contents. Ninety-two single-nucleotide polymorphisms (SNP) and 35 simple-sequence repeat (SSR) loci were significantly correlated with ADF and NDF contents, respectively, and six genetic loci associated with ADF and NDF contents were detected using both types of markers. We identified three candidate genes on chromosome A05 related to ADF content, including genes encoding chitinase-like protein 2 (CTL2) and two trichome birefringence-like 41 s (TBL41s). Seven genes on chromosomes A03 and A04 were related to NDF content, including genes encoding glycosyl hydrolase (GH), reversibly glycosylated polypeptide 1 (RGP), irregular xylem 12 (IRX12), trichome birefringence-like 34 (TBL34), galacturonosyltransferase 7 (GAUT7), cytokinesis defective 1 (CYT1), and LOB domain-containing protein 15 (LBD15). These candidate genes encode factors that likely participate in secondary cell wall formation and lignocellulose biosynthesis.
Conclusions

These findings lay the foundation for identifying genes related to forage quality traits and improving the efficiency of forage utilization in rapeseed, which will be beneficial for breeding new varieties for high-quality forage with low lignocellulose content.
A study on mental health status among the staff in a designated hospital for COVID- 19
Authors: Wang Bangfang , Sun Jianjun , Gao Feng , Chen Jun , Shi Lei , Li Lanying , Tang Yongjun , Wang Kun , Lu Hongzhou 
Published: April 2020

Abstract
Background: We investigate the mental health status of all the staff members who worked for the designated hospital during the initial stage of COVID-19, so as to understand the severity of mental health problems, and analyze the risk factors.Methods: Through the patients health questionnaire-9(PHQ-9) and panic disorder severity scales(PDSS), we surveyed the status of depression and panic disorder of the staff who participated in the prevention and treatment of COVID-19 in designated hospital in the early stage of epidemic. The data is described by the number of cases (percentage), median and interquartile range. The chi square test was used for categorical variables and the rank sum test was used for continuous variables. The risk factors of severe depression or panic disorder were analyzed by binary logistic regression test.Results: Totally 702 questionnaires were sent out and 694(98.9%) was received and qualified, the median score of PHQ-9 among all the staff was 1 (IQR,0-4), 143(20.6%) of them had depression, 39 (5.6%) had serious depression; the median score of PDSS was 2 (IQR,0-5), 81 (11.7%) of them had panic disorder and 47(6.7%) of them had severe panic disorder; Among the people in different work lines, the first-line staff scored the highest: PHQ-9 score was 4 (0-8); PDSS score was 4 (1-9), which were significantly higher than the second-line and third-line staff (P<0.001). Multivariate logistic regression analysis showed that the adjusted risk of severe depression in first-line staff was 6.63 fold(P < 0.001); the risk of severe panic disorder was 2.62 fold (P=0.003) higher than that of non-first line group.Conclusions: Many staff in the designated hospital for COVID-19 have mental health problems. Among them, first-line workers are a high-risk group with severe depression and panic disorder, and further psychological intervention is needed for them.
Downregulation of S100A2 alleviates pulmonary fibrosis through inhibiting wnt/β-catenin signaling pathway
Authors: Huang Guichuan , Zhang Jing , Qing Gang , Liu Daishun , Wang Xin , Chen Yi , Li Yishi , Guo Shuliang 
Published: April 2020

Abstract
Background：Pulmonary fibrosis (PF) is a progressive and lethal disease with poor prognosis. S100A2 plays an important role in the progression of cancer. However, the role of S100A2 in PF has not been reported yet. In this study, we explored the potential role of S100A2 in PF and its potential molecular mechanisms. Methods: First, we analyzed S100A2 expression of patients with PF by retrieving RNA-sequencing datasets from Gene Expression Omnibus (GEO) database. Next, we detected the expression of S100A2 in patients with PF using quantitative real time PCR (qRT-PCR). Then, S100A2 expression was determined with or without the treatment of transforming growth factor-β1 (TGF-β1) in A549 cells. Epithelial-mesenchymal transition (EMT) biomarkers, including E-cadherin，vimentin, and α smooth muscle actin (α-SMA), were identified using qRT-PCR and western blot. Finally, the relevant signalling pathway indicators were detected by western blot. Results: Increased expression of S100A2 was first observed in lung tissues of PF patients. Meanwhile, we found that downregulation of S100A2 inhibited the TGF-β1-induced EMT in A549 cells. Mechanically, TGF-β1 up-regulated β-catenin and phosphorylation of GSK-3β, which was blocked by silencing S100A2 in vitro. Conclusion: These findings demonstrate that downregulation of S100A2 alleviate pulmonary fibrosis via inhibiting EMT. S100A2 is a promising potential target for further understanding the mechanism and developing strategy for the treatment of PF and other EMT-associated disease.
Relationship between the Functional Oral Intake Scale and Self-efficacy Scale among Patients with Cancer: A Cross-sectional study
Authors: Matsuda Yuhei , Karino Masaaki , Kanno Takahiro 
Published: April 2020

Abstract
Background: A few studies suggest that self-efficacy pertaining to swallowing ability can lead to dysphagia. Therefore, this cross-sectional study verified the relationship between oral health-related self-efficacy and dysphagia severity during cancer treatment. Methods: Participants included patients undergoing treatment for cancer at Shimane University Hospital, Shimane, Japan, and those receiving outpatient treatment at the hospital’s Oral Care Center between August 2018 and April 2019. In all, 203 participants enrolled in the study and completed the Functional Oral Intake Scale (FOIS), Self-efficacy Scale for Advanced Cancer (SEAC), and Oral Health-related Self-Efficacy Scale for Patients with Cancer (OSEC). Results: A multivariate analysis showed a statistically significant correlation between the low FOIS score category and the SEAC subscales of Activities of Daily Living Self-efficacy (ADE) (odds ratio 1.04, 95% confidence interval [CI] 1.00–1.07) and Symptom Coping Self-efficacy (SCE) (odds ratio 0.61, 95% [CI] 0.42–0.88). Based on the Jonckheere-Terpstra test, the SEAC and OSEC scores tended to increase as the FOIS category progressed. Conclusion: These findings suggest that self-efficacy played an important role in dysphagia and it may affect the severity of dysphagia.