bluuebunny/crossref_metadata_2025_split huggingface dataset:
Phytochemical and Biological Evaluation of Cyanus celikhanensis Extract: An in Silico and in Vitro Approach
Authors: Baş Topcu Kübra Sena , Sağ Vildan , Genç Nusret , Celik Ahmet , İmamoğlu Rizvan , Kisa Dursun 
Published: December 2024

ABSTRACTHerbal products are valuable biological resources of bioactive compounds expressed as secondary metabolites. This study aimed to explore the potential biological properties and phenolic content of Cyanus celikhanensis Kaya et al. extract, which has no data on pharmacological properties. A quantitative phytochemical analysis of C. celikhanensis was performed using a triple quadrupole LC/MS system. In vitro pharmacological tests, which included antioxidant, antimicrobial, antiproliferative, and 10 medicinal enzyme inhibition, were accompanied by in silico studies. The extract of C. celikhanensis exhibited promising inhibitory effect against HMG‐CoA reductase (IC50: 0.724 µg mL−ˡ), α‐glucosidase (IC50: 68 µg mL−ˡ), and α‐amylase (IC50: 114 µg mL−ˡ) enzymes. The extract presented a cytotoxic effect (130.4 µg mL−ˡ), antibacterial potency against Enterococcus faecalis (591.2 ± 2.7 µg mL−ˡ), and ABTS scavenging activity (12.32 µg mL−ˡ) closer to that of BHT (8.12 ± 0.096 µg mL−ˡ). The quantitative phytochemical analysis revealed that the major compounds were keracyanin chloride (72.56 µg mL−ˡ) and quinic acid (12.54 µg mL−ˡ). The molecular insertion studies showed that the major compound has low binding energy, and the biological capacity of the plant extract might be attributable to its leading compounds, especially keracyanin. According to the first comprehensive in vitro and in silico analysis, the study data show that C. celikhanensis has potential biological activities.
Hypersine H, One Undescribed Xanthone From the Hypericum elodeoides Choisy With Anti‐Neuroinflammation Activity
Authors: He Shoulun , Liu Xiangzhong , Wang Tingjiya , Li Jingdian , Lin Yingting , Zhou Mi , Sun Cuiling , Ding Rong , Wang Guanghui , Chen Hai‐Feng , Tian Wen‐Jing 
Published: December 2024

ABSTRACTOne undescribed xanthone, hypersine H (1), together with three known analogs, 3,7‐dihydroxy‐1‐methoxyxanthone (2), 1,7‐dihydroxy‐5,6‐dimethoxyxanthone (3), and 1,5‐dihydroxy‐6,7‐dimethoxyxanthone (4), were isolated from the whole plant of Hypericum elodeoides Choisy. Their structures, including absolute configurations, were unambiguously elucidated by HR‐ESI–MS, extensive NMR spectroscopy, and quantum chemical calculation of electronic circular dichroism (ECD) method. Moreover, the anti‐neuroinflammation activities of isolated compounds were evaluated. As a result, Compounds 1–2 decreased the production of nitric oxide (NO) in LPS‐stimulated mouse BV2 microglia. Further mechanism study indicated that 1 and 2 exerted anti‐neuroinflammation effects by suppressing the expression of pro‐inflammatory enzymes, including inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)‐2. Above all, compounds 1–2 showed promise as useful therapeutic agents in the treatment of neurodegenerative diseases caused by neuroinflammation.
Enzymatic Hydrolysis of Residual Soybean Oil with Simultaneous Separation Using Membrane
Authors: Pacheco Bruna Jeanne Soares , Vieira Rafael Bruno , Cardoso Vicelma Luiz 
Published: December 2024

AbstractThis study investigates the simultaneous separation process using a batch reactor coupled to a cellulose acetate membrane for the enzymatic hydrolysis of residual soybean oil. The membrane was manufactured with 30 s pre‐evaporation time and 1‐mm thickness. Their morphologies and wettability were analyzed. Pichia pastoris lipase was characterized based on temperature and pH and was applied to the hydrolysis of residual soybean oil at 45 °C and pH 7 in batches, with and without simultaneous separation. The results show that the process efficiency with the cellulose acetate membrane resulted in 55.58 % ± 2.78 % free fatty acids in enzymatic hydrolysis, compared with 39.72 % ± 1.99 % without the membrane. The membrane also achieved 100 % oil rejection.
The Impact of Single Amino Acid Insertion on the Supramolecular Assembly Pathway of Aromatic Peptide Amphiphiles
Authors: Higuchi Ayato , Som Arka , Wakabayashi Rie , Goto Masahiro , Kamiya Noriho , Besenius Pol 
Published: December 2024

AbstractUnderstanding the mechanism of self‐assembly driven by non‐covalent interactions is crucial for designing supramolecular materials with desired properties. Here we investigate the self‐assembly of aromatic peptide amphiphiles, Fmoc‐L2QG and Fmoc‐L3QG using a combination of spectroscopic, transmission electron and superresolution optical microscopy techniques. Our results show that Fmoc‐L2QG leads to concentration‐dependent assembly, forming fibrous assemblies at low concentrations and supramolecular droplets via liquid‐liquid phase separation (LLPS) at higher concentrations. Mechanical activation using for example ultrasonication triggered the transition from metastable droplets to fibre morphologies of Fmoc‐L2QG. In contrast, Fmoc‐L3QG followed both on‐pathway and off‐pathway routes, resulting in the formation of fibrous morphologies regardless of concentration. Seeding experiments revealed that homo‐seeds of the same peptide sequence accelerated the on‐pathway process, while hetero‐seeds of a mismatched peptide sequences accelerated the off‐pathway process, highlighting the competing nature of the complex assembly profile. These findings demonstrate the significant impact of single amino acid insertion on the supramolecular assembly process of oligopeptide monomers, and highlight the potential for controlling the structure and dynamics of peptide materials. Pathway engineering of oligopeptide building blocks and multidomain supramolecular monomers will open new avenues in tailor‐made and customizable supramolecular biomaterials.
Covalently Grafting of Ethylenediamine onto a 2D Sub‐stoichiometric COF for Enhanced CO2/N2 Separation†
Authors: Sun Shixin , Luo Guanglun , Peng Yongwu 
Published: December 2024

Comprehensive SummaryThe post‐synthetic modification of two‐dimensional sub‐stoichiometric covalent organic frameworks (COFs) for gas separation, particularly for the efficient removal of CO2 from CO2/N2 mixtures, is an area of growing interest yet remains relatively unexplored. In this study, we report the precise tuning of functional groups in sub‐stoichiometric COFs to enhance CO2 capture performance. A sub‐stoichiometric COF with free aldehyde groups (HPCOF) was designed and synthesized, followed by covalent modification via ethylenediamine (EDA) grafting, yielding HPCOF‐EDA. This post‐modification strategy significantly increased CO2 adsorption capacity (55.7 cm3·g–1 at 298 K and 1 bar) and CO2/N2 selectivity (58), primarily attributed to the formation of additional N—H···O interactions. These findings demonstrate the potential of functional engineering to broaden the application prospects of sub‐stoichiometric COFs in gas adsorption and separation technologies.
Relationship Between Left Ventricular Ejection Fraction and ICD‐10 Codes Among Patients Hospitalized With Heart Failure
Authors: Gluckman Ty J. , Chiu Shih‐Ting , Rider Deanna , Tseng Phil K. , Mudd James O. , Remick Joshua D. , Granowitz Craig , Carroll Amy , Sikirica Slaven , Canonico Mario E. , Hsia Judith , Bonaca Marc P. 
Published: December 2024

ABSTRACTIntroductionWhile left ventricular ejection fraction (LVEF) represents an important means by which to classify patients with heart failure (HF), relatively little is known about the distribution of LVEFs among patients hospitalized for HF based on their International Classification of Disease (ICD)‐10 code.MethodsWe performed a retrospective cross‐sectional analysis of patients admitted to a large integrated health system within the western US between January 1, 2018 and October 1, 2022 with a principal diagnosis of HF (defined by ICD‐10 codes: I50.2, systolic HF; I50.3, diastolic HF; I50.4, combined systolic and diastolic HF; I11.0, hypertensive heart disease with HF; and I13.0 and I13.2, hypertensive heart disease with HF and chronic kidney disease).ResultsOver nearly 5 years, 61,238 HF hospitalizations occurred, of which 49,772 (81%) had a LVEF available by echocardiography within the preceding 3 months. Whereas most patients hospitalized with systolic HF (n = 2220) as well as systolic and diastolic heart failure (n = 1582) had an LVEF ≤ 40% (86.2% and 74.8%, respectively), most patients hospitalized with diastolic HF (n = 1542) had an LVEF ≥ 50% (94.0%) (Figure). A much greater range of LVEFs were noted for those with hypertensive heart disease with HF (n = 18,092) and hypertensive heart disease with HF and CKD (n = 26,336) (Figure).ConclusionWhile there was relatively good concordance between LVEF and the ICD‐10 code‐defined HF type for systolic HF, diastolic HF, and systolic and diastolic HF, these codes represent a small subset (~10%) of total HF hospitalizations.
Diastereoselective Cascade Double Michael Addition to Access Bridged Coumarins, Oxindoles and Spirooxindoles: A Sustainable Strategy for Synthesis of Anticancer Molecules
Authors: Battula Shravani , Bhumannagari Haripriya , Ambadipudi S. S. S. S. Sudha , Andugulapati Sai Balaji , Nayani Kiranmai 
Published: December 2024

AbstractAn efficient and concise synthesis of highly functionalized bridged coumarins has been developed through a diastereoselective double Michael addition reaction of p‐quinols with various 4‐hydroxy coumarins under catalyst‐free conditions in H2O‐DMSO (8 : 2). The method has been applied to oxindoles for the synthesis of a variety of bridged‐oxindoles and bridged‐spiroxindoles in presence of a DABCO base using H2O−EtOH (8 : 2) as solvent medium. The strategy is simple, highly atom economical as there is no by‐product and environmentally benign (E‐factor=0.1–0.9). The synthesized compounds were screened against triple‐negative breast cancers and found that bridged coumarin (3 a) and oxindole (5 d) compounds exhibit potent anti‐cancer activity at 6.6 and 8.8 μM (IC50) concentrations respectively. Further analysis revealed that 3 a and 5 d caused elevated early and total apoptosis by arresting the MDA‐MB‐468 cells in G2/M phase of the cell cycle. Overall, our results demonstrate that bridged coumarin (3 a) and oxindole (5 d) compounds‐based approach attenuates the cancer progression and may pave a path for the translational outcome.
Fabrication of Ferulic Acid‐Cellulose Nanocrystal Enhanced Stretchable and Antibacterial Hydrogels
Authors: Li Fangyu , Wang Yajie , Xu Luchun , Li Xiaxue , Song Wangcheng , Mao Yanyong , Zhou Jia , Shi Hao 
Published: December 2024

AbstractHydrogels are widely utilized in biomaterials, medicine, and food science due to their versatile properties. This study developed a ferulic acid (FA) and cellulose nanocrystals (CNC) hydrogel within a polyacrylamide matrix, polymerized using ammonium persulfate and crosslinked with N‐methylenebisacrylamide. The FA@CNC hydrogel demonstrated exceptional tensile ductility and elasticity. Antibacterial evaluations against Escherichia coli and Staphylococcus aureus revealed significant efficacy, with activity increasing proportionally to FA concentration. Swelling studies indicated a maximum equilibrium swelling ratio of 1210 % after 36 h, showcasing the hydrogel's ability to undergo substantial expansion while maintaining its original shape and structural integrity. It was indicated that the synthesized hydrogels were capable of absorbing large volumes of exudate while preserving a moist environment conducive to accelerated wound healing. Scanning electron microscopy analysis confirmed a regular and dense microstructure, which contributes to the hydrogel's mechanical stability and robustness. The optimized preparation process developed in this study resulted in hydrogels with significantly enhanced performance. These findings underscore the hydrogel's superior mechanical and functional properties, paving the way for innovative applications across biomaterials, medical, and food‐related industries.
Remote Sensing Data Reveal a Significant Reduction in the Area of the Nesting Habitat of Rafetus euphraticus in the Tigris River, Southeastern Turkey
Authors: Biricik Murat , Safi Kamran , Turğa Şemsettin 
Published: December 2024

ABSTRACTThe Euphrates Softshell Turtle (Rafetus euphraticus) is an endangered freshwater turtle native to the Tigris‐Euphrates river system. Habitat destruction caused by dams and sand mining poses a major threat to the species. This study quantitatively assesses the occurrence of sandy areas in the upper Tigris in Turkey as a key component of their nesting habitat, utilizing remote sensing data. An ensemble approach was employed, applying and combining Generalized Additive Models (GAM), Generalized Boosting Models (GBM), Generalized Linear Models (GLM), and Random Forests (RF) for classification. The models indicate that, compared to 2018, the occurrence of sandbanks—assumed to be crucial nesting habitat for this species—was reduced by 41% in 2022 following the construction of a large dam. Additionally, sand mining and flooding of adjacent lands have fragmented and degraded the remaining sandbanks. The survival of the Turkish population of the Euphrates Softshell Turtle requires immediate and effective action.
Warm Spring Days are Related to Shorter Durations of Reproductive Phenophases for Understory Forest Herbs
Authors: Miller Chelsea N. , Stuble Katharine L. 
Published: December 2024

ABSTRACTAs plants continue to respond to global warming with phenological shifts, our understanding of the importance of short‐lived heat events and seasonal weather cues has lagged relative to our understanding of plant responses to broad shifts in mean climate conditions. Here, we explore the importance of warmer‐than‐average days in driving shifts in phenophase duration for spring‐flowering woodland herbs across one growing season. We harnessed the combined power of community science and public gardens, engaging more than 30 volunteers to monitor shifts in phenology (documenting movement from one phenophase to the next) for 198 individual plants of 14 species twice per week for the 2023 growing season (March—October) across five botanic gardens in the midwestern and southeastern US. Gardens included the Holden Arboretum, Kirtland, OH; Dawes Arboretum, Newark, OH; Chicago Botanic Garden, Glencoe, IL; Missouri Botanical Garden, St. Louis, MO; and Huntsville Botanical Garden, Huntsville, AL. We tested: (1) that higher‐than‐average daily temperatures (deviation from 30‐year historical mean daily temperatures for each location) would be related to truncated phenophase durations; and (2) that phenophase durations would vary among species. Our findings support both hypotheses. We documented significant inverse relationships between positive deviations in daily temperature from historic means (e.g., warmer‐than‐average days) and durations of three reproductive phenophases: “First Bud,” “First Ripe Fruit,” and “Early Fruiting.” Similar (non‐significant) trends were noted for several other early‐season phenophases. Additionally, significant differences in mean phenophase durations were detected among the different species, although these differences were inconsistent across plant parts (vegetative, flowering, and fruiting). Results underscore the potential sensitivity of understory herb reproductive phenophases to warmer‐than‐average daily temperatures early in the growing season, contributing to our understanding of phenological responses to short‐term heat events and seasonal weather cues.
Range Extension of the Popeye Catalufa (Pristigenys serrula, Gilbert 1891) to Central Chile During the “El Niño” Southern Oscillation (ENSO) 2023–2024
Authors: Asorey Cynthia M. , Cuevas Jeremías , Larraín María Angélica , Araneda Cristian 
Published: December 2024

ABSTRACTWe report the presence of Pristigenys serrula Gilbert 1891 off Zapallar (−32.568253, −71.464225), central Chile, during the ENSO 2023–2024 event. Morphology and a partial fragment of the mitochondrial markers cytochrome oxidase 1 (COX1) and 16S rDNA identified one specimen fished by artisanal fishermen. This report extends the known southern range limit of P. serrula by 9° of latitude (~1000 km) to central Chile (−32°) during strong negative ENSO conditions.
BAFF Blockade Attenuates B Cell MALT Formation in Conditional Nlrc5‐Deficient Mice With Helicobacter felis Infection
Authors: Tong Dongmei , He Yuqi , Haile Shambel Araya , Lee Zoe , Le Lena H. M. , Emery Jack , Wray‐McCann Georgie , Chonwerawong Michelle , Philpott Dana J. , Hertzog Paul J. , Schneider Pascal , Ferrero Richard L. , Ying Le 
Published: December 2024

ABSTRACTHelicobacter infection is a key cause of gastric B cell mucosa–associated lymphoid tissue (MALT) lymphoma. This study examined the role of B cell–activating factor (BAFF), a major driver of B cell proliferation and many B cell disorders, in this malignancy using a model in which conditional knockout mice for NOD‐like receptor family CARD domain‐containing 5 (Nlrc5) are infected with Helicobacter felis. Gastric BAFF production was significantly increased in H. felis–infected Nlrc5mø‐KO mice compared to wild‐type. Blocking BAFF signalling, before or after the onset of Helicobacter‐induced gastritis, significantly reduced MALT development, with fewer gastric B cell follicles and reduced gland hyperplasia. BAFF blockade also reshaped the immune cell landscape in the stomach, resulting in fewer CD4+ T cells, Tregs, macrophages and dendritic cells. Using a cell culture model, we identified the protein‐coding BAFF transcripts that are upregulated in NLRC5‐deficient macrophages stimulated with either H. felis or the NLRC5 agonist, lipopolysaccharide. Among the upregulated variants, TNFSF13B (BAFF)‐206 acts as a transcription factor and is reported to enhance BAFF production in autoimmune diseases and cancer. Altogether, these findings implicate the NLRC5–BAFF signalling axis in Helicobacter‐induced B cell MALT lymphoma, highlighting BAFF inhibition as a potential therapeutic approach.
Pseudo‐silapnictene and Bis(pseudo‐silapnictene) – Hyperconjugatively Stabilized Heavier Analogues of Imine and Ethane‐1,2‐diimine
Authors: Muhammed Shahila , Parameswaran Pattiyil 
Published: December 2024

AbstractThe equilibrium geometry, bonding, and proton affinity of the pseudo‐silapnictenes and the bis(pseudo‐silapnictenes) are explored using computational quantum mechanical calculations carried out at M06/def2‐TZVPP//BP86‐D3(BJ)/def2‐SVP level of theory. The pseudo‐silapnictene 1E features a dicoordinated, monovalent group‐15 center having two lone pairs of σ‐ and π‐local symmetry. Both the lone pairs are stabilized by hyperconjugative donation to the silylene fragment. The extent of hyperconjugative stabilization of π‐type lone pair is higher than that of σ‐type lone pair and is comparable to classical Si−E π‐bonds. Accordingly, the former interactions can be considered as pseudo‐π‐bonding interactions. The strength of the pseudo‐π‐bond reduces as the group‐15 element changes from nitrogen to bismuth. Hence, 1E can be considered as heavier analogues of imine. Even though these lone pairs are stabilized by hyperconjugative interactions, they are very reactive as indicated by the very high values of first and second proton affinities. In the bis(pseudo‐silapnictene) 2E, two Si−E pseudo‐π‐bonds formed by hyperconjugative interactions are delocalized over the E−Si−Si−E skeleton. Since the π‐bonds are majorly localized on the group‐15 element, the extent of delocalization is quite less than that of the classical π‐delocalized system. Nevertheless, the bis(pseudo‐silapnictene) can be considered as the heavier analogue of ethane‐1,2‐diimine.
Plant‐Based Meat Alternatives Intake and Its Association With Health Status Among Vegetarians of the UK Biobank Volunteer Population
Authors: Navratilova Hana F. , Whetton Anthony D. , Geifman Nophar 
Published: December 2024

ABSTRACTConsumption of plant‐based meat alternatives (PBMAs) within the vegetarian population is increasing. This study assessed the relationship between PBMA intake and health markers using the UK Biobank cohort. Participants were categorized into vegetarian PBMA consumers and vegetarian PBMA nonconsumers. Nonparametric statistical tests were used to evaluate differences in participants’ characteristics, food intake, and 30 blood biochemistry measures after assessing data distributions. Metabolomics (168 metabolites) and proteomics (2923 proteins) data were further examined to identify significant differences between the two participant groups. Relative risks (RRs) for 45 chronic diseases and mental conditions were calculated using Poisson regression. Sensitivity analysis accounted for sociodemographic factors, and the proportion of energy from ultra‐processed food (UPF) intake was determined. No substantial differences in sodium, free sugar, total sugar, or saturated fatty acids intake between vegetarian PBMA consumers and nonconsumers were found. However, PBMA consumers exhibited higher blood pressure (130/79 and 129/78 mmHg for consumer and nonconsumer groups, respectively) and elevated C‐reactive protein (CRP) levels (1.76 ± 3.12 and 1.57 ± 3.17 mg/L for consumer and nonconsumer groups, respectively). Metabolite and protein abundance analysis showed no notable differences. Pathway enrichment analysis suggested that PBMAs may influence immune reactions through cell signaling pathways. PBMA consumers had a 42% increased risk of depression (p = 0.03) and a 40% reduction in irritable bowel syndrome (IBS) risk (p = 0.02), compared to nonconsumers. In conclusion, while no clear health risks or benefits were associated with PBMA consumption in vegetarians, the higher risk of depression, elevated CRP, and lower apolipoprotein A levels in PBMA consumers suggest potential inflammatory concerns that warrant further investigation.
Unveiling the medicinal potential of edible mushroom polysaccharides: Structure, function, and therapeutic insights
Authors: Khaskheli Shahzor Gul , Liu Ying , Khaskheli Ashfaque Ahmed , Dong Xiaobo 
Published: December 2024

AbstractEdible mushrooms have been a staple in diets worldwide for centuries, prized for their distinct flavors, versatility in the kitchen, and potential health benefits. Among the various bioactive compounds found in mushrooms, polysaccharides have garnered significant attention due to their diverse therapeutic applications. This review aims to provide a comprehensive overview of the medicinal properties of polysaccharides in edible mushrooms, highlighting their potential for future medicinal advancements. Over the past 3 decades, numerous polysaccharides and polysaccharide‐protein complexes have been isolated from mushrooms, many of which are currently used or being investigated as therapeutic agents. Studies suggest that polysaccharides derived from mushrooms may be effective in cancer treatment. The primary components of these polysaccharides in edible mushrooms include beta‐glucans, heteroglycans, and glycoproteins, all of which enhance the mushrooms' potential as medicinal agents.