bluuebunny/crossref_metadata_2025_split huggingface dataset:
MiR-128-3p Alleviates Spinal Cord Ischemia/Reperfusion Injury Associated Neuroinflammation and Cellular Apoptosis via SP1 Suppression in Rat
Authors: Wang Dan , Chen Fengshou , Fang Bo , Zhang Zaili , Dong Yan , Tong Xiangyi , Ma Hong 
Published: December 2020

BackgroundNeuroinflammation and cellular apoptosis caused by spinal cord ischemia/reperfusion (I/R) injury result in neurological dysfunction. MicroRNAs (miRs) have crucial functions in spinal cord I/R injury pathogenesis according to previous evidences. Herein, whether miR-128-3p contributes to spinal cord I/R injury by regulating specificity protein 1 (SP1) was assessed.MethodsA rat model of spinal cord I/R injury was established by occluding the aortic arch for 14 min. Then, miR-128-3p’s interaction with SP1 was detected by dual-luciferase reporter assays. Next, miR-128-3p mimic and inhibitor, as well as adenovirus-delivered shRNA specific for SP1 were injected intrathecally for assessing the effects of miR-128-3p and SP1 on rats with spinal cord I/R injury. SP1, Bax and Bcl-2 expression levels in I/R injured spinal cord tissues were evaluated by Western blotting, while IL-1β, TNF-α, and IL-6 were quantitated by ELISA. Tarlov scores were obtained to detect hind-limb motor function. Evans blue (EB) dye extravasation was utilized to examine blood–spinal cord barrier (BSCB) permeability. Terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) staining was performed for neuronal apoptosis assessment.ResultsMiR-128-3p expression was decreased, while SP1 amounts were increased in rat spinal cord tissue specimens following I/R. SP1 was identified as a miR-128-3p target and downregulated by miR-128-3p. MiR-128-3p overexpression or SP1 silencing alleviated I/R-induced neuroinflammation and cell apoptosis, and improved Tarlov scores, whereas pretreatment with miR-128-3p inhibitor aggravated the above injuries.ConclusionOverexpression of miR-128-3p protects neurons from neuroinflammation and apoptosis during spinal cord I/R injury partially by downregulating SP1.
CircRNA_000864 Upregulates B-cell Translocation Gene 2 Expression and Represses Migration and Invasion in Pancreatic Cancer Cells by Binding to miR-361-3p
Authors: Huang Linsheng , Han Junxiang , Yu Huifan , Liu Jialing , Gui Lili , Wu Zhengkun , Zhao Xinxu , Su Shiqi , Fu Gaohang , Li Fei 
Published: December 2020

BackgroundPancreatic cancer is a fatal disease with a very poor prognosis due to its characteristic insidious symptoms, early metastasis, and chemoresistance. Circular RNAs (circRNAs) are involved in the development of pancreatic cancer.AimHence, the aim of this study is to elucidate the mechanism of circRNA_000864 in regulating BTG2 expression in pancreatic cancer by binding to miR-361-3p.MethodsCircRNA_000864, miR-361-3p, and BTG2 expression patterns in the pancreatic cancer tissues were detected by RT-qPCR. Correlation among circRNA_000864, miR-361-3p, and BTG2 was evaluated by RNA-pull down assay, RNA Immunoprecipitation assay, and dual-luciferase reporter gene assay. After ectopic expression and depletion experiments, 5-ethynyl-2′-deoxyuridine assay, Transwell assay, and flow cytometry were employed to assess the cell proliferation, migration and invasion, cell cycle, and apoptosis. Xenotransplantation of nude mice was conducted to detect the effects of circRNA_000864, miR-361-3p, and BTG2 on tumor growth.ResultsCircRNA_000864 and BTG2 were poorly expressed, and miR-361-3p was highly expressed in the pancreatic cancer tissues. CircRNA_000864 bound to miR-361-3p could target BTG2. Cell proliferation, migration, and invasion were inhibited, and the cell cycle arrest and apoptosis were stimulated after overexpression of circRNA_000864 or BTG2 or downregulation of miR-361-3p. Overexpression of circRNA_000864 or downregulation of miR-361-3p also decreased the tumor growth in vivo.ConclusionsConjointly, our findings elicited that the overexpression of circRNA_000864 could promote BTG2 expression to inhibit pancreatic cancer development by binding to miR-361-3p, which represents an appealing therapeutic target for the treatment of pancreatic cancer.
An Integrated Network, RNA Sequencing, and Experiment Pharmacology Approach Reveals the Active Component, Potential Target, and Mechanism of Gelsemium elegans in the Treatment of Colorectal Cancer
Authors: Wang Lin , Xu Hai-li , Liang Jing-wei , Ding Ying-ying , Meng Fan-hao 
Published: December 2020

In this study, a combination of network pharmacology, bioinformatics analysis, molecular docking and transcriptomics was used to investigate the active ingredient and potential target ofGelsemium elegansin the treatment of colorectal cancer. Koumine was screened as the active component by targeting PDK1 through network pharmacology and reverse docking. RNA-Seq, enrichment analysis and validation experiment were then further employed to reveal koumine might function in inhibiting Akt/mTOR/HK2 pathway to regulate cell glycolysis and detachment of HK2 from mitochondria and VDAC-1 to activate cell apoptosis bothin vitroandin vivo. In the present study, we provide a systematical approach for the identification of effective ingredient and potential target of herbal medicine. Our results have important implication for the intensive study of koumine as novel anticancer agents for colorectal cancer and could be supportive in its further structural modification.
Impact of Opioid Consumption in Patients With Functional Gastrointestinal Disorders
Authors: Melchior Chloé , Desprez Charlotte , Wuestenberghs Fabien , Leroi Anne-Marie , Lemaire Antoine , Goucerol Guillaume 
Published: December 2020

Objective: We aimed to determine the burden of opioid consumption in a cohort of patients with functional gastrointestinal disorders.Methods: All patients diagnosed with functional gastrointestinal disorders and referred to our university hospital were evaluated from 2013 to the beginning of 2019. Irritable bowel syndrome and functional dyspepsia diagnoses were determined according to Rome criteria and severity according to irritable bowel syndrome severity scoring system. Vomiting was quantified using a 5-point Likert scale, and constipation severity was measured using the Knowles-Eccersley-Scott-Symptom questionnaires. Quality of life was quantified by the GastroIntestinal Quality of Life Index. Patients were categorized as being treated on a chronic basis with either tramadol, step II opioids, step III opioids or as being opioid-free.Results: 2933 consecutive patients were included. In our cohort, 12.5% had only irritable bowel syndrome, 39.3% had only functional dyspepsia, 24.9% had a combination of both, and 23.4% had other functional gastrointestinal disorders. Among them, the consumption of tramadol, step II (tramadol excluded) and step III opioids was 1.8, 1.3 and 0.3 % respectively in 2013 and 4.3, 3.4 and 1.9% in 2018 (p &lt; 0.03). Opioid consumption was associated with increased vomiting (p = 0.0168), constipation (p &lt; 0.0001), symptom severity (p &lt; 0.001), more altered quality of life (p &lt; 0.0001) and higher depression score (p = 0.0045).Conclusion: In functional gastrointestinal disorders, opioid consumption has increased in the last years and is associated with more GI symptoms (vomiting, constipation and GI severity), higher depression and more altered quality of life.
The Extent to Which Obesity and Population Nutrition Are Considered by Institutional Investors Engaged in Responsible Investment in Australia - A Review of Policies and Commitments
Authors: Robinson Ella , Parker Christine , Carey Rachel , Sacks Gary 
Published: December 2020

IntroductionResponsible investment (RI), in which environmental, social and governance (ESG) considerations are incorporated into investment decision making, is a potentially powerful tool for increasing corporate accountability and improving corporate practices to address broad societal challenges. Whilst the RI sector is growing, there is limited understanding of the extent to which pressing social issues, such as obesity and unhealthy population diets, are incorporated within RI decision making. This study aimed to investigate the extent to which obesity prevention and population nutrition are considered by Australian institutional investors engaged in responsible investment.MethodsA desk-based review was conducted of investment approaches of prominent Australian asset managers and superannuation funds identified as engaged in responsible investment. Relevant information on the incorporation of ESG issues related to obesity and population nutrition was extracted for each investor, drawing on websites, published policy documents and annual reports. Strategies were categorized as: (1) negative/exclusionary screening; (2) positive/best-in-class screening; (3) norms-based screening; (4) ESG integration; (5) sustainability-themed investing; (6) impact/community investing; and (7) corporate engagement and shareholder action. These strategies were compared across investors and by themes related to obesity and population nutrition.ResultsEighteen of the 35 investors indicated that they applied investment strategies that considered issues related to obesity and population nutrition. The most commonly identified strategy was ESG integration (n = 12), followed by sustainability-themed investing (n = 6), and positive screening (n = 4). The ways in which obesity and population nutrition were considered as part of these approaches included relatively high-level general health considerations (n = 12), considerations around the healthiness of food company product portfolios (n = 10), and consideration of specific company nutrition policies and practices (n = 4). The specificity and depth to which RI strategies were disclosed varied.ConclusionThere is significant potential for investment decisions to contribute to efforts to address key social issues, such as obesity and unhealthy diets. Some institutional investors in Australia have recognized the potential importance of incorporating obesity- and population nutrition-related issues into decision-making processes. However, the extent to which these considerations translate into investment decisions and their impact on companies in the food sector warrant further exploration.
Nursing Students' Perceptions, Knowledge, and Preventive Behaviors Toward COVID-19: A Multi-University Study
Authors: Albaqawi Hamdan Mohammad , Alquwez Nahed , Balay-odao Ejercito , Bajet Junel Bryan , Alabdulaziz Hawa , Alsolami Fatmah , Tumala Regie B. , Alsharari Abdalkarem F. , Tork Hanan M. M. , Felemban Ebaa Marwan , Cruz Jonas Preposi 
Published: December 2020

Background:Knowledge, perception, and preventive behavior should be considered in the planning of effective educational interventions for the coronavirus disease of 2019 (COVID-19) pandemic and in increasing awareness about the health risks brought about by this disease. This research aimed to assess knowledge, perceptions, and preventive behavior toward the COVID-19 infection among student nurses.Methods:The study has quantitative, descriptive, and cross-sectional design. A convenience sample of 1,226 student nurses from seven universities in Saudi Arabia was surveyed from March 22 to April 4, 2020. A four-part online survey on demographic characteristics, perceptions, knowledge, and preventive behavior of Saudi student nurses was carried out.Results:Nearly all students were aware of the outbreak (99.2%), and most of them received information on COVID-19 primarily from social media (71.0%). Over three-fourths of the students were confident that the government (89.1%) and Ministry of Health (MOH) (86.5%) were doing a good job responding to the COVID-19 outbreak in the country. The overall average score in the knowledge questionnaire was 9.85 (SD = 1.62, range = 0–12), which is equivalent to 82.1%. The majority of the students always performed most of the preventive behavior identified in the survey, except “washing hands with soap and water for at least 20 s after blowing my nose, coughing, or sneezing” (39.2%) and “daily cleaning and disinfecting frequently touched surfaces” (41.6%). Being female, being in the fourth year, and gaining good perceived knowledge were associated with high actual COVID-19 knowledge. University, gender, age, academic level, and perceived COVID-19 knowledge were the associated factors.Conclusions:The findings of this study have provided baseline information on the current state of Saudi nursing students' perceptions, knowledge, and preventive behavior toward COVID-19 as the crisis is happening. The findings revealed some areas that should be focused on by nursing education, as well as health agencies, to ensure that the students have adequate knowledge and correct preventive behavior.
NEW TENDENCIES IN A SCHOOL DESIGNING IN DIFFERENT COUNTRIES
Authors: Chechel' I. , Chechel' I. 
Published: December 2020

School is the most important element in modernization and innovative development of a State in XIX century. The face of school is changed significantly. It becomes a center with a rich by intellectual, creative and sport life for school-students and their parents. At the same time, school has meet new social and town-planning requirements. Object of the study is an experience of UK, USA, Finland, Belgium, Japan and China concerning a building designing for regular schools.
The research methodology consists of a challenge problem, study and analyzing of experience in different countries, analysis the problem about designing experience in Russia.
As a result of the study, it was found that the specifics of the most countries is to create special and unique buildings where school-students can get knowledge in accordance with the school curriculum for a comfortable environment. That leads to communication and personal enhancement. Thus, schools are integrating centers for the development of school-student, their parents, and surround habitants.
INDUSTRIAL BUILDINGS OF RUSSIAN NORTH
Authors: Shopina E. , Orlov N. , Anpilogova E. 
Published: December 2020

Nowadays, architecture and design mainly is oriented on development of new technologies. In these conditions it is important to use traditional styles and techniques. This knowledge can help to form a basis for future architects and designers as well as for professionals is to know current ideas and projects.
Object of research is Russian wooden architecture, in particular, industrial buildings of Russian North; historical background of formation of this style. These knowledges is an essential base for professional mentality of architects and designers.
Results of the study: it was found that specificity of Russian wooden architecture consists of combination of traditions in this region.
According to the author’s opinion, understanding of the individual architecture of this region, the specifics of the construction of wooden buildings and untraditional mentality of ancient architects, opens up a large field for investigation of the culture of this region.
Regional Citrate Anticoagulation Protocol for Patients with Presumed Absent Citrate Metabolism
Authors: Szamosfalvi Balazs , Puri Vidhit , Sohaney Ryann , Wagner Benjamin , Riddle Amy , Dickinson Sharon , Napolitano Lena , Heung Michael , Humes David , Yessayan Lenar 
Published: December 2020

Background
Regional citrate anticoagulation (RCA) is not recommended in patients with shock or severe liver failure. We designed a protocol with personalized precalculated flow settings for patients with absent citrate metabolism that abrogates risk of citrate toxicity, and maintains neutral continuous KRT (CKRT) circuit calcium mass balance and normal systemic ionized calcium levels.

Methods
A single-center prospective cohort study of patients in five adult intensive care units triaged to the CVVHDF-RCA “Shock” protocol.

Results
Of 31 patients included in the study, 30 (97%) had AKI, 16 (52%) had acute liver failure, and five (16%) had cirrhosis at the start of CKRT. The median lactate was 5 mmol/L (interquartile range [IQR], 3.2–10.7), AST 822 U/L (IQR, 122–2950), ALT 352 U/L (IQR, 41–2238), total bilirubin 2.7 mg/dl (IQR, 1.0–5.1), and INR two (IQR, 1.5–2.6). The median first hemofilter life censored for causes other than clotting exceeded 70 hours. The cumulative incidence of hypernatremia (Na >148 mM), metabolic alkalosis (HCO3- >30 mM), and hypophosphatemia (P<2 mg/dl) were one out of 26 (4%), zero out of 30 (0%), and one out of 30 (3%), respectively, and were not clinically significant. Mild hypocalcemia occurred in the first 4 hours in two out of 31 patients, and corrected by hour 6 with no additional Ca supplementation beyond the per-protocol administered Ca infusion. The maximum systemic total Ca (tCa; mM)/ionized Ca (iCa; mM) ratio never exceeded 2.5.

Conclusions
The Shock protocol can be used without contraindications and is effective in maintaining circuit patency with a high, fixed ACDA infusion rate to blood flow ratio. Keeping single-pass citrate extraction on the dialyzer >0.75 minimizes the risk of citrate toxicity even in patients with absent citrate metabolism. Precalculated, personalized dosing of the initial Ca-infusion rate from a table on the basis of the patient’s albumin level and the filter effluent flow rate maintains neutral CKRT circuit calcium mass balance and a normal systemic iCa level.
In Vivo Entombment of Bacteria and Fungi during Calcium Oxalate, Brushite, and Struvite Urolithiasis
Authors: Saw Jessica J. , Sivaguru Mayandi , Wilson Elena M. , Dong Yiran , Sanford Robert A. , Fields Chris J. , Cregger Melissa A. , Merkel Annette C. , Bruce William J. , Weber Joseph R. , Lieske John C. , Krambeck Amy E. , Rivera Marcelino E. , Large Timothy , Lange Dirk , Bhattacharjee Ananda S. , Romero Michael F. , Chia Nicholas , Fouke Bruce W. 
Published: December 2020

Background
Human kidney stones form via repeated events of mineral precipitation, partial dissolution, and reprecipitation, which are directly analogous to similar processes in other natural and manmade environments, where resident microbiomes strongly influence biomineralization. High-resolution microscopy and high-fidelity metagenomic (microscopy-to-omics) analyses, applicable to all forms of biomineralization, have been applied to assemble definitive evidence of in vivo microbiome entombment during urolithiasis.

Methods
Stone fragments were collected from a randomly chosen cohort of 20 patients using standard percutaneous nephrolithotomy (PCNL). Fourier transform infrared (FTIR) spectroscopy indicated that 18 of these patients were calcium oxalate (CaOx) stone formers, whereas one patient formed each formed brushite and struvite stones. This apportionment is consistent with global stone mineralogy distributions. Stone fragments from seven of these 20 patients (five CaOx, one brushite, and one struvite) were thin sectioned and analyzed using brightfield (BF), polarization (POL), confocal, super-resolution autofluorescence (SRAF), and Raman techniques. DNA from remaining fragments, grouped according to each of the 20 patients, were analyzed with amplicon sequencing of 16S rRNA gene sequences (V1–V3, V3–V5) and internal transcribed spacer (ITS1, ITS2) regions.

Results
Bulk-entombed DNA was sequenced from stone fragments in 11 of the 18 patients who formed CaOx stones, and the patients who formed brushite and struvite stones. These analyses confirmed the presence of an entombed low-diversity community of bacteria and fungi, including Actinobacteria, Bacteroidetes, Firmicutes, Proteobacteria, and Aspergillus niger. Bacterial cells approximately 1 μm in diameter were also optically observed to be entombed and well preserved in amorphous hydroxyapatite spherules and fans of needle-like crystals of brushite and struvite.

Conclusions
These results indicate a microbiome is entombed during in vivo CaOx stone formation. Similar processes are implied for brushite and struvite stones. This evidence lays the groundwork for future in vitro and in vivo experimentation to determine how the microbiome may actively and/or passively influence kidney stone biomineralization.
La inscripción SEG 34.1532 y la de Alejandría en Egipto ptolemaico
Authors: Druille Paola 
Published: December 2020

En este artículo nos proponemos analizar la terminología registrada en SEG 34.1532, especialmente las referencias que aluden a la existencia de la γερουσία en Egipto ptolemaico y sus funciones administrativas vinculadas con el gimnasio de Alejandría. Intentaremos traducir SEG 34.1532 y rastrear el término γερουσία y el vocabulario relacionado con este consejo, y examinar los datos sobre su existencia y potestad en Egipto ptolemaico, además de su conexión con el gimnasio alejandrino. La lectura de esta inscripción y su contextualización histórica y social nos permitirán discutir también acerca de los términos técnicos ligados con la γερουσία en el período ptolemaico tardío.
Terminalidad de la educación secundaria y formación para el trabajo en la Ciudad de Buenos Aires: el Bachillerato con Orientación Profesional
Authors: Belmes Armando , Rodrigo Ismael Álvaro 
Published: December 2020

El propósito de esta comunicación es presentar algunos de los resultados de un estudio exploratorio, enmarcado en la Unidad de Evaluación Integral de la Calidad y Equidad Educativa (UEICEE), cuyo objetivo fue examinar un dispositivo de inclusión educativa, el Bachillerato con Orientación Profesional, destinado a adolescentes que, por distintas circunstancias asociadas a la vulnerabilidad social y educativa, han transitado trayectorias discontinuas en el sistema educativo. Este dispositivo se implementa desde 2015 en seis Centros de Formación Profesional de la Ciudad de Buenos Aires, articulando la enseñanza de las asignaturas básicas de la formación general de nivel Medio con un trayecto curricular de Formación Profesional inicial correspondiente a un sector de actividad específico.
El estudio analiza la estructura curricular y la organización institucional del dispositivo, y explora las particularidades de su implementación en cada uno de los centros, a partir de una estrategia metodológica cualitativa y cuantitativa que combina distintos tipos de fuentes, métodos y técnicas. Los mismos permiten advertir, a la par del trabajo “artesanal” docente y el profundo compromiso de los actores institucionales, una brecha entre el enunciado y la práctica. Esta brecha se constituye en una “zona gris” que evidencia las limitaciones para implementar un régimen académico flexible y superar la fragmentación disciplinar tradicional de la escuela secundaria en general y en particular de la técnica-profesional.
Characterizing Rayleigh wave and longitudinal shear wave propagation for measurements of elastic moduli using optical coherence elastography
Authors: Wang Chongyang , Zhu Jiang , Zhu Lianqing , Liu Jianting , Fan Fan , Yang Qiang , Zhang Fan 
Published: December 2020

Abstract
Optical coherence elastography based on the measurements of elastic wave speeds can quantify elastic moduli in the wave propagation directions and provide high-resolution elasticity maps for tissue. Here, we simultaneously visualized a longitudinal shear wave propagating along the depth and a Rayleigh wave propagating in the lateral direction, and analyzed the propagation characteristics of the elastic waves, including group speeds, frequency spectra, and frequency-dependent wave speeds. The analysis of the propagation characteristics demonstrates the reliability of the elasticity quantification by measuring different types of speeds of the elastic waves.