bluuebunny/crossref_metadata_2025_split huggingface dataset:
NONO and tumorigenesis: More than splicing
Authors: Feng Peifu , Li Ling , Deng Tanggang , Liu Yan , Ling Neng , Qiu Siyuan , Zhang Lin , Peng Bo , Xiong Wei , Cao Lanqin , Zhang Lei , Ye Mao 
Published: March 2020

AbstractThe non‐POU domain‐containing octamer‐binding protein NONO/p54nrb, which belongs to the Drosophila behaviour/human splicing (DBHS) family, is a multifunctional nuclear protein rarely functioning alone. Emerging solid evidences showed that NONO engages in almost every step of gene regulation, including but not limited to mRNA splicing, DNA unwinding, transcriptional regulation, nuclear retention of defective RNA and DNA repair. NONO is involved in many biological processes including cell proliferation, apoptosis, migration and DNA damage repair. Dysregulation of NONO has been found in many types of cancer. In this review, we summarize the current and fast‐growing knowledge about the regulation of NONO, its biological function and implications in tumorigenesis and cancer progression. Overall, significant findings about the roles of NONO have been made, which might make NONO to be a new biomarker or/and a possible therapeutic target for cancers.
Intravitreal conbercept improves outcome in patients undergoing vitrectomy for proliferative diabetic retinopathy: A systematic review and meta‐analysis
Authors: Pranata Raymond , Vania Amelinda 
Published: March 2020

AbstractObjectivesTo evaluate the latest evidence concerning the efficacy of conbercept on vitrectomy for proliferative diabetic retinopathy (PDR) and its efficacy compared to control and other antivascular endothelial growth factor.MethodsWe performed a systematic literature search on topics that assess the role of conbercept in patients undergoing vitrectomy for PDR from inception to November 2019, using PubMed, EuropePMC, Cochrane Central Database, ProQuest, ScienceDirect, and Clinicaltrials.gov. Two researchers independently searched literature, extracted data, and evaluated the risk of bias. RevMan 5.3 and StataMP 16 software were used to perform data analysis.ResultsThere were 699 cases (eyes) from eight studies. Baseline best‐corrected visual acuity (BCVA) was better in the control group compared to conbercept group (mean difference [MD] = 0.13, I2 = 0%). A greater BCVA improvement was observed in the conbercept group after 1‐month (MD = –0.27, I2 = 1%), 3‐month (MD = –0.28, I2 = 0%), and 6‐month (MD = –0.20, I2 = 78%) follow‐up. The need for endodiathermy (odds ratio [OR] = 0.20, I2 = 0%) and silicone oil tamponade use (OR = 0.59, I2 = 72%) and intraoperative bleeding (OR = 0.11, I2 = 33%) was lower in conbercept group. Postoperative early (OR = 0.22, I2 = 0%) and late (OR = 0.47, I2 = 0%) vitreous hemorrhage was lower in conbercept group. There was no significant difference in BCVA improvement and intraoperative outcome between conbercept and ranibizumab.ConclusionsIntravitreal conbercept was associated with a more significant BCVA improvement, better intraoperative outcome, and less postoperative vitreous hemorrhage compared to no conbercept.
A new species of Hyphessobrycon Durbin (Characiformes: Characidae) from the western Amazon basin in Colombia and Peru
Authors: García‐Alzate Carlos Arturo , Lima Flavio , Taphorn Donald Charles , Mojica Jose Ivan , Urbano‐Bonilla Alexander , Teixeira Tulio Franco 
Published: March 2020

AbstractHyphessobrycon chiribiquete n. sp. is described from the Río Caquetá drainage in Colombia and the Río Ucayali drainage in Peru, western Amazon. The new species is diagnosed from its congeners by having the following combination of characters: a conspicuous narrow midlateral stripe, starting on the sides of the body behind the opercle near the lateral line; lateral stripe overlapped anteriorly with a vertically elongated humeral blotch; inner premaxillary teeth pentacuspid; margin of anal fin falcate in mature males.
A new species of trout from the northern drainages of Euphrates River, Turkey (Salmoniformes: Salmonidae)
Authors: Turan Davut , Kalaycι Gökhan , Bektaş Yusuf , Kaya Cüneyt , Bayçelebi Esra 
Published: March 2020

AbstractSalmo fahrettini, a new species, is distributed in the northern tributaries of the Euphrates River. It differs from other Salmo species in adjacent waters by a combination of the following characters: a greyish body; one black spot behind the eye and on the cheek; three to six black spots on the opercle; numerous black spots on the back (missing on the predorsal area), flank and middle part of body, surrounded by a roundish white ring; red spots in the median part of the body, surrounded by a roundish white ring; short and narrow maxilla; increase in the number of black and red spots with an increase in size; adipose fin medium size, no or rarely one red spot at its posterior edge; 109–116 lateral line scales; 27–30 scale rows between dorsal‐fin origin and lateral line; 20–23 scale rows between the lateral line and anal‐fin origin; maxilla length 8.8–10.0% standard length in males, 8.8–9.6 in females.
Morphological and physiological traits of Mediterranean sticklebacks living in the Camargue wetland (Rhone river delta)
Authors: Rind Khalid , Rodriguez‐Barucg Quentin , Nicolas Delphine , Cucchi Patricia , Lignot Jehan‐Hervé 
Published: March 2020

AbstractThree‐spined sticklebacks (Gasterosteus aculeatus L.) living at the southern limit of the species distribution range could possess specific morphological and physiological traits that enable these fish to live at the threshold of their physiological capacities. Morphological analysis was carried out on samples of sticklebacks living in different saline habitats of the Camargue area (Rhone delta, northern Mediterranean coast) obtained from 1993 to 2017. Salinity acclimation capacities were also investigated using individuals from freshwater‐low salinity drainage canals and from mesohaline–euryhaline lagoons. Fish were maintained in laboratory conditions at salinity values close to those of their respective habitats: low salinity (LS, 5‰) or seawater (SW, 30‰). Fish obtained from a mesohaline brackish water lagoon (BW, 15‰) were acclimated to SW or LS. Oxygen consumption rates and branchial Na+/K+‐ATPase (NKA) activity (indicator of fish osmoregulatory capacity) were measured in these LS or SW control fish and in individuals subjected to abrupt SW or LS transfers. At all the studied locations, only the low‐plated “leiurus” morphotype showed no spatial or temporal variations in their body morphology. Gill rakers were only longer and denser in fish sampled from the LS–freshwater (FW) drainage canals. All fish presented similar physiological capacities. Oxygen consumption rates were not influenced by salinity challenge except in SW fish transferred to LS immediately and 1 h after transfer. However, and as expected, gill NKA activity was salinity dependent. Sticklebacks of the Camargue area sampled from habitats with contrasted saline conditions are homogenously euryhaline, have low oxygen consumption rates and do not appear to experience significantly greater metabolic costs when challenged with salinity. However, an observed difference in gill raker length and density is most probably related to the nutritional condition of their habitat, indicating that individuals can rapidly acclimatize to different diets.
Whole‐genome next‐generation sequencing and phylogenetic characterization of viral haemorrhagic septicaemia virus in Korea
Authors: Hwang Jee Youn , Ahn Sang Jung , Kwon Mun‐Gyeong , Seo Jung Soo , Hwang Seong Don , Jee Bo Young 
Published: March 2020

AbstractWhole‐genome next‐generation sequencing was used to investigate the local evolution of viral haemorrhagic septicaemia virus, a serious pathogen affecting economically important fish such as rainbow trout and turbot in Europe and olive flounder in Asia. Sequence analysis showed that all isolates were genotype IVa, but could be classified further into four subgroups (K1–K4). In addition, genomic regions encompassing the nucleoprotein, phosphoprotein, matrix protein and non‐virion protein genes, as well as the seven non‐coding regions, were relatively conserved, whereas glycoprotein and RNA‐dependent RNA polymerase genes were variable in the coding region. Taken together, the data demonstrate that whole‐genome next‐generation sequencing may be useful for future surveillance, prevention and control strategies against viral haemorrhagic septicaemia.
Equivalent efficacies of reverse hybrid and concomitant therapies in first‐line treatment of Helicobacter pylori infection
Authors: Hsu Ping‐I , Tsay Feng‐Woei , Kao John Y , Peng Nan‐Jing , Tsai Kuo‐Wang , Tsai Tzung‐Jiun , Kuo Chao‐Hung , Kao Sung‐Shuo , Wang Huay‐Min , Chen Yan‐Hua , Shie Chang‐Bih , Wu Deng‐Chyang ,   Taiwan Acid‐related Disease & Microbiota (TARD‐M) Consortium
Published: March 2020

AbstractBackground and AimConcomitant therapy is a recommended first‐line treatment for Helicobacter pylori infection in most national or international consensuses. Reverse hybrid therapy is a modified 14‐day concomitant therapy without clarithromycin and metronidazole in the final 7 days. This study aims to test whether 14‐day reverse hybrid therapy is non‐inferior to 14‐day concomitant therapy in the first‐line treatment of H. pylori infection.MethodsHelicobacter pylori‐infected adult patients were randomly assigned to receive either reverse hybrid therapy (dexlansoprazole 60 mg o.d. plus amoxicillin 1 g b.d. for 14 days, and clarithromycin 500 mg plus metronidazole 500 mg b.d. for initial 7 days) or concomitant therapy (dexlansoprazole 60 mg once o.d. plus amoxicillin 1 g, clarithromycin 500 mg, and metronidazole 500 mg b.d. for 14 days). H. pylori status was assessed 6 weeks after the end of treatment.ResultsHelicobacter pylori‐infected participants (n = 248) were randomized to receive either 14‐day reverse hybrid therapy (n = 124) or 14‐day concomitant therapy (n = 124). Intention‐to‐treat analysis demonstrated that the two therapies had comparable eradication rate (95.2% vs 93.5%; 95% confidence interval, −4.0% to 7.4%; P = 0.582). However, reverse hybrid therapy had a much lower frequency of adverse events than concomitant therapy (20.2% vs 38.7%, P = 0.001). The two therapies exhibited comparable drug adherence (93.5% vs 87.9%, P = 0.125).ConclusionsFourteen‐day reverse hybrid therapy and 14‐day concomitant therapy are equivalent in efficacy for the first‐line treatment of H. pylori infection. However, reverse hybrid therapy has fewer adverse events compared with concomitant therapy.
Thyroid Function and Sarcopenia: Results from the ELSA‐Brasil Study
Authors: Szlejf Claudia , Suemoto Claudia K. , Janovsky Carolina C.P.S. , Barreto Sandhi M. , Diniz Maria de Fátima H.S. , Lotufo Paulo A. , Bensenor Isabela M. 
Published: March 2020

OBJECTIVESWe aimed to investigate the association of subclinical thyroid disease and thyroid hormone levels with sarcopenia and its defining components in community‐dwelling middle‐aged and older adults without overt thyroid dysfunction.DESIGNCross‐sectional study.SETTINGActive and retired employees from public institutions located in six Brazilian cities.PARTICIPANTSA total of 6974 participants from the ELSA‐Brasil study's second wave, aged 50 years and older, without overt thyroid dysfunction and with complete data for exposure, outcome, and covariates.METHODSSerum levels of thyrotropin (TSH), free thyroxine, and free triiodothyronine (FT3) were measured and divided in quintiles for the analyses. Participants were classified with euthyroidism, subclinical hypothyroidism, and subclinical hyperthyroidism. Muscle mass was assessed by bioelectrical impedance analysis and muscle strength by handgrip strength. Sarcopenia was defined according to the Foundation for the National Institutes of Health criteria. Possible confounders included sociodemographic characteristics, clinical conditions, and lifestyle. Analyses were performed separately for middle‐aged and older adults (≥65 y).RESULTSThe frequencies of sarcopenia, low muscle mass, low muscle strength, subclinical hypothyroidism, and subclinical hyperthyroidism were 1.5%, 20.8%, 3.8%, 9.1%, and .9%, respectively. Subclinical thyroid dysfunction was not associated with sarcopenia and its defining components. Among older adults, TSH had a U‐shaped association with sarcopenia and low muscle strength. The odds ratios (ORs) (95% confidence intervals [CIs]) for the associations of the first, second, fourth, and fifth quintile with sarcopenia, respectively, were 5.18 (1.47‐18.28), 6.28 (1.82‐21.73), 4.12 (1.15‐14.76), and 4.81 (1.35‐17.10), and with low muscle strength was (OR (95% CI) for the first, second, and fifth quintiles, respectively: 1.43 (1.16‐5.07), 2.07 (1.24‐4.70), and 2.18 (1.03‐4.60). Additionally, FT3 had a negative association with muscle mass in both age strata.CONCLUSIONSubtle thyroid hormone alterations are associated with sarcopenia or its defining components in middle‐aged and older adults without overt thyroid dysfunction. J Am Geriatr Soc 68:1545‐1553, 2020.
Stimulation of collagenesis by poly‐L‐lactic acid (PLLA) and ‐glycolide polymer (PLGA)‐containing absorbable suspension suture and parallel sustained clinical benefit
Authors: Goldberg David J. 
Published: March 2020

AbstractBackgroundThough the collagen‐stimulating capacity of poly‐L‐lactic acid (PLLA) is well established, no data are available on collagen stimulation by the PLLA/PLGA within absorbable suspension sutures used for tissue repositioning.AimsTo characterize collagen stimulation by absorbable suspension sutures through examination of patient biopsies and assess severity of nasolabial folds (NLF) over time.Patients/MethodsSingle‐center, open‐label, prospective study of 25 healthy volunteers treated with absorbable suspension sutures for descent of midface tissues. Punch biopsies were collected and evaluated for total collagen content. Subject and investigators evaluated NLF severity using the Nasolabial Fold Wrinkle Assessment Score (NLF‐WAS). Seventeen of 25 patients completed the collagenesis portion of the study.ResultsCollagen deposition was apparent at day 90 and increased significantly compared with baseline at days 180 and 270 and between days 90 and 180. Subject‐ and investigator‐reported NLF‐WAS were significantly improved at all post‐treatment time points through day 270.ConclusionsAbsorbable suspension sutures stimulate collagen in areas immediately adjacent to the path of the PLLA/PLGA monofilament and cones, providing mechanistic support for improvement in appearance observed in clinical studies. This neocollagenesis, coupled with repositioning by the suture's cones, makes absorbable suspension sutures a valuable tool for patients seeking facial rejuvenation.
Successful surgical treatment of cardiac metastasis from uterine leiomyosarcoma: A case report and literature review
Authors: Maebayashi Aki , Nagaishi Masaji , Nakajima Takahiro , Hata Mitsumasa , Xiaoyan Tang , Kawana Kei 
Published: March 2020

AbstractUterine leiomyosarcoma is a malignant soft tissue tumor resembling uterine fibroma clinically and is difficult to diagnose preoperatively. Since metastatic cardiac tumors are very rare and asymptomatic, most cardiac metastases are detected at autopsy after death due to other diseases. A 49‐year‐old woman presented with menorrhagia and anemia, and a uterine tumor. Total hysterectomy was performed for the uterine tumor. Histopathological examination revealed the tumor to be a leiomyosarcoma. Postoperative computed tomography showed multiple lung metastases and a metastatic cardiac tumor. The cardiac tumor, which was located within and almost entirely occluded the right ventricle, was 49 × 26 mm. To prevent sudden death, cardiac tumorectomy was performed semi‐emergently. Chemotherapy was initiated in the early postoperative period, and the patient currently maintains a complete response. Cases with lung and cardiac metastases rarely undergo surgical resection of metastatic tumors. However, emergent surgical resection of cardiac metastatic tumors should be considered to prevent sudden death.
Effect of dietary tannins on milk yield and composition, nitrogen partitioning and nitrogen use efficiency of lactating dairy cows: A meta‐analysis
Authors: Herremans Sophie , Vanwindekens Frédéric , Decruyenaere Virginie , Beckers Yves , Froidmont Eric 
Published: March 2020

AbstractTannins are secondary plant compounds which have been extensively studied in order to improve the nitrogen use efficiency (NUE) of ruminants. A meta‐analysis was performed of 58 in vivo experiments comparing milk yield, composition and nitrogen metabolism of lactating dairy cows fed diets with or without tannins. The meta‐analysis shows that tannins have no impact on corrected milk yield, fat and protein content or NUE (p > .05). However, tannins reduce ruminal ammoniacal nitrogen (N) production by 16% (from 10.95 to 8.47 mg/dl on average), milk urea by 9% (from 15.82 to 14.03 mg/dl) and urinary N excretion (−11%; p < .05). This is compensated for by a lower apparent N digestibility (61.51% with dietary tannins compared to 66.17% without). The effect of tannin on N metabolism parameters increases with tannin dose (p < .05). The shift from urinary to faecal N may be beneficial for environment preservation, as urinary N induces more harmful emissions than faecal N. From a farmer's perspective, tannins seem unable to increase fat‐ and protein‐corrected milk yield or reduce feed protein requirements and thus have no direct economic benefit. Potentially less costly than tannin extracts, forage or by‐products naturally rich in tannins could still be useful to reduce the environmental impact of ruminant protein feeding.
Polyphyly and species delimitation of Picea brachytyla (Pinaceae) based on population genetic data
Authors: Lyu Le‐Ke , Wang Dong‐Lei , Li Long , Zhu Ying‐Ying , Jiang De‐Chun , Liu Jian‐Quan , Xu Xiao‐Ting 
Published: March 2020

AbstractAccurate species delimitation is fundamental to biodiversity conservation. The endangered spruce Picea brachytyla (Franch.) E. Pritz. was suggested to be polyphyletic based on a limited number of samples in previous studies. To evaluate polyphyly of P. brachytyla, we sampled 139 individuals from 16 populations across most of its distributional range, plus representatives of two related species, Picea likiangensis (Franch.) E. Pritz. and Picea wilsonii Mast. We sequenced 13 nuclear loci and three chloroplast and two mitochondrial loci for the following species delimitation. Phylogenetic analyses of nuclear loci grouped all individuals of P. brachytyla from Sichuan and Chongqing into one distinct lineage and those from Yunnan and Tibet (southern distribution) nested within the P. likiangensis species complex. Structure analyses confirmed this result. Networks of chloroplast DNA haplotypes similarly showed that P. brachytyla from the southern distribution nested within the P. likiangensis species complex, whereas haplotypes for the northern distribution comprised a separate and well‐supported lineage. These results suggest that P. brachytyla from the southern distribution is a part of the P. likiangensis species complex and should be removed from P. brachytyla. Our study highlights the utility of population genetic evidence in delimitating endangered species and understanding the conservation status of such species.
The effect of transobturator tape operation on sexual functions of a patient with stress urinary incontinence and patient's spouse
Authors: Oğlak Süleyman Cemil , Obut Mehmet 
Published: March 2020

AbstractPurposeThis prospective study aimed to investigate the effects of transobturator tape (TOT) procedure on patients with stress urinary incontinence (SUI) and their spouses' sexual function.Material and methodsA total of 157 patients with SUI who underwent TOT operation between January 2017 and May 2019 and their spouses were included. All patients enrolled filled out the Incontinence Impact Questionnaire (IIQ‐7), the Urogenital Distress Inventory (UDI‐6), the Female Sexual Function Index (FSFI), and patients' spouses filled out International Index of Erectile Function (IIEF‐5) before surgery and 6 months after the surgery.ResultsThe mean value of IIQ‐7 and UDI‐6 questionnaires in the sixth month after the TOT surgery was lower than the mean scores of these questionnaires before surgery (P < .001). The mean value of the FSFI score was 21.84 ± 1.76 before the surgery and 25.43 ± 1.84 in the sixth month after the surgery (P < .001). There was a significant improvement in desire, arousal, lubrication, orgasm, satisfaction, and pain scores on the FSFI domains (P < .001, <.001, <.001, <.001, <.001 and <.05, respectively). The mean value of the IIEF‐5 score of patients' spouses was 48.31 ± 5.23 before surgery and 57.87 ± 7.22 in the sixth month after the surgery (P < .001). Significant improvement was observed in IIEF‐5 score regarding sexual desire, intercourse satisfaction, and overall satisfaction domains (P = .012, .002, and .006, respectively).ConclusionTOT surgery significantly improves the sexual functions of both patients and their spouses.
A review of mammal eradications on Mediterranean islands
Authors: Capizzi Dario 
Published: March 2020

Abstract

Impacts of alien invasive species on island communities and ecosystems may be even more detrimental than on the mainland. Therefore, since the 1950s, hundreds of restoration projects have been implemented worldwide, with the aim of controlling or eradicating alien species from islands. To date, no review has been focused on eradication on Mediterranean islands. To fill the gap, I reviewed the available information concerning mammal eradications so far carried out on Mediterranean islands, examining the details of several aspects of project implementation and monitoring.

I obtained data for 139 attempted eradications on 107 Mediterranean islands in eight countries, with Greece, Italy, and Spain accounting for the highest number. Eradication projects targeted 13 mammal species. The black rat Rattus rattus was the target of over 75% of the known attempted eradications in the Mediterranean Basin; other species targeted were feral goat Capra hircus, house mouse Mus musculus, European rabbit Oryctolagus cuniculus, and domestic cat Felis catus. The most widely adopted technique was poisoning (77% of all eradications), followed by trapping (15%) and hunting (4%). However, techniques were largely target‐specific.

The average failure rate was about 11%. However, this percentage varied according to the specific mammalian order, and eradications of Carnivora failed more often than those of other mammals. Among rodents, house mouse eradication attained a very high failure rate (75%). Reinvasion occurred after 15% of successful eradications.

A better understanding of the motivations of animal rights activists may improve the chance of success when eradicating charismatic or domesticated species. Furthermore, it is crucial to collect data and case studies about reinvasions, in order to strengthen biosecurity programmes following eradication. As in other parts of the world, the next frontier in alien mammal management on Mediterranean islands concerns the eradication of invasive species from inhabited islands.