bluuebunny/crossref_metadata_2025_split huggingface dataset:
A lightweight segmentation and lineage tracking tool for noisy, low frame rate 4D fluorescence microscopy data
Authors: Qureshi Haseeb Khalid , Magony Andor , Hadzhiev Yavor , Wozniak Kacper , Jasiulewicz Alexandra , Müller Ferenc , Sik Attila 
Published: November 2020

Abstract4D fluorescence microscopy allows the study of spatiotemporal cell dynamics in embryonic development in unprecedented detail, yet the uneven scattering of light within developing embryos presents challenges in discerning fine details. We present a tool which pre-processes largein vivo4D microscopy datasets and can then track the movements and lineage histories of rapidly dividing embryonic stem cells. This solution offers a robust, simple segmentation technique to segment high intensity fluorescent features in highly scattered datasets, such as from deep within a developing zebrafish embryo. This tool then offers lineage tracing functionality by tracking rapidly dividing nuclei and their progeny, while also accounting for the jumps rapidly moving features make between frames in low frame rate data. Based on the user having prior knowledge of their imaging subject, this tool has applications in datasets with limitations with signal to noise, and frame rate. It can provide information regarding the position and movement of rapidly dividing nuclei. We demonstrate this pipeline in developing early zebrafish embryos.
CRISPR-Cas13 mediated Knock Down inDrosophilacultured cells
Authors: Viswanatha Raghuvir , Zaffagni Michela , Zirin Jonathan , Perrimon Norbert , Kadener Sebastian 
Published: November 2020

AbstractManipulation of gene expression is one of the best approaches for studying gene functionin vivo. CRISPR-Cas13 has the potential to be a powerful technique for manipulating RNA expression in diverse animal systemsin vivo, includingDrosophila melanogaster. Studies using Cas13 in mammalian cell lines for gene knockdown showed increased on-target efficiency and decreased off-targeting relative to RNAi. Moreover, catalytically inactive Cas13 fusions can be used to image RNA molecules, install precise changes to the epitranscriptome, or alter splicing. However, recent studies have suggested that there may be limitations to the deployment of these tools inDrosophila, so further optimization of the system is required. Here, we report a new set of PspCas13b and RfxCas13d expression constructs and use these reagents to successfully knockdown both reporter and endogenous transcripts inDrosophilacells. As toxicity issues have been reported with high level of Cas13, we effectively decreased PspCas13b expression without impairing its function by tuning down translation. Furthermore, we altered the spatial activity of both PspCas13b and RfxCas13d by introducing Nuclear Exportation Sequences (NES) and Nuclear Localization Sequences (NLS) while maintaining activity. Finally, we generated a stable cell line expressing RfxCas13d under the inducible metallothionein promoter, establishing a useful tool for high-throughput genetic screening. Thus, we report new reagents for performing RNA CRISPR-Cas13 experiments inDrosophila, providing additional Cas13 expression constructs that retain activity.
Transfer of Antibiotic Resistance Genes from Gram-positive Bacterium to Gram-negative Bacterium
Authors: Manohar Prasanth , Shanthini Thamaraiselvan , Bozdogan Bulent , Lundborg Cecilia Stalsby , Tamhankar Ashok J , Palaniyar Nades , Ramesh Nachimuthu 
Published: November 2020

AbstractThe emergence of antibiotic resistance due to uncontrolled use of antibiotics in non-humans, poses a major threat for treating bacterial infections in humans. Added to this is the possibility of transfer of resistance from Gram-positive bacteria to Gram-negative bacteria. Therefore, the possibility of resistance gene transfer from a non-human originated pathogenic bacterium to a pathogenic bacterium infecting humans needs evaluation. In this study, poultry litter samples collected from Tamil Nadu, India were screened for the presence of meropenem- and cefotaxime-resistantStaphylococcus sciuri. Standard microbiological techniques and 16S rRNA analysis were used to confirmS. sciuri. In the resistant isolates, resistance genes such asblaNDM-1,blaOXA-48-like,blaKPC,blaVIM,blaIMPandblaCTX-Mwere screened. Transconjugation studies were performed using donor,S. sciuriand recipient,E. coliAB1157 (Strr). A total of 26 meropenem-resistant and 24 cefotaxime resistantS. sciuriwere isolated from poultry litter samples. The presence ofblaNDM-1(n=2),blaIMP(n=8),blaCTX-M-9(n=5) andblaCTX-M-2(n=1) was detected. Transconjugation results confirmed thatS. sciuricarrying plasmid-borne resistance geneblaNDM-1conjugated toE. coliAB1157. The transferability of resistance genes fromS. sciuritoE. colicould be another possible reason for spread of antibiotic-resistant bacteria in humans.
Early entrants attract better customer evaluations: evidence from the digital camera industry
Authors: Kirjavainen Johanna , Mäkinen Saku J. , Dedehayir Ozgur 
Published: November 2020

PurposeIn addition to pioneering, empirical work on entry order increasingly addresses fast followers and laggards and the potential advantages they are able to capture. There is also a growing consensus in the academia, that current measures of firm performance used in the entry order literature to study these advantages are inadequate. This study analyzes the relationship between entry order and customer evaluations, which, depicting the performance of the firm's products in the market, are used as a proxy for firm performance.Design/methodology/approachThe study is set in the digital camera industry, analyzing entries into each new technology level, in terms of the sensor resolution of compact and bridge cameras. The complete dataset consisted of 1,816 digital camera models introduced between January 1996 and December 2017. The data are analyzed using hierarchical multiple linear regression.FindingsThe study finds evidence of early-mover advantage for the compact product category. In the compact camera consumer market, both first-movers and fast followers outperform late movers. Furthermore, the difference in performance in comparison to laggards is greater for first-movers than for fast followers. However, in the bridge category which consists of a more heterogeneous set of products, no significant entry-order effects are detected.Originality/valueThe results clearly indicate that there exists an early mover advantage. Furthermore, the results are not consistent across different product categories within an industry; hence, caution needs to be exercised when analyzing industry dynamics and entry order effects. Finally, our novel conceptualization of firm performance measured as online customer evaluation add new opportunities to investigate firm success
Intercultural service encounters: a systematic review and a conceptual framework on trust development
Authors: Vrontis Demetris , Leonidou Erasmia , Christofi Michael , Kaufmann Hans Ruediger , Kitchen Philip J. 
Published: November 2020

PurposeA significant body of research has now been accumulated in the intercultural service encounter (ICSE) literature. However, no study to date has provided scholars and practitioners with a systematic review to map and better understand the ICSE domain.Design/methodology/approachTo fill this gap, the authors systematically review and critically examine the state of academic research on ICSE.FindingsBased on a systematic review of 31 journal articles published over the last two decades, the results illustrate that ICSE research is a vibrant and rapidly growing stream of the broader international business domain, and it is topically and methodologically diverse. This review also identifies significant knowledge gaps related to the adoption of different theoretical orientations by researchers examining ICSE at different levels of analysis, a lack of contextual positioning, as well as poor methodological rigor.Originality/valueBased on the findings, the authors introduce a multilevel and multidisciplinary conceptual framework that integrates the concepts of emotional intelligence (EI) and intercultural communication competence (ICC) as the key variables that explain trust development during the interaction between two key culturally different stakeholders: service providers (employees) and service receivers (customers). Finally, the authors discuss the contributions and implications for both academics and practitioners.
Affective intensities: Emotion, race, gender and the push and pull of bodies
Authors: Schmidt Kimberly McDavid , Beucher Rebecca 
Published: November 2020

PurposeThis study aims to investigate the ways affective intensities arise in the intra-actions within an assemblage (three Black girls, objects such as computers and hoodies, institutionalized discourse associated with race and successful participation in schools) as the girls create multimodal responses to literature. This paper shows how the intra-actions among the girls and material objects produce affective intensities or new ways of being and becoming through which youth reauthor themselves as central and peripheral participants.Design/methodology/approachThe authors present an illustrative case of the ways girls’ embodied literacy identities emerge when Jillian, Isa, and Rhianna intra-act with materials in an assemblage that includes their material-discursive positionings through qualitative and multimodal interaction analysis.FindingsThe analysis describes the ways the girls agentively participate through play, composing and moments of becoming (fluid subjectivities) that include emotive acts such as acts of solidarity, loving connectedness and possible frustration that inform who counts and who can be successful in the classroom.Research limitations/implicationsThis single case study gives a descriptive, in-depth analysis of the ways affective intensities emerge as three girls respond to literature to understand their embodied and discursive practices within the composing process.Originality/valueTo fully understand agency and the students’ emergent subjectivities, the authors combine embodiment and material-discursive analysis to understand affective intensities that evolve during three Black girls’ composing processes and the ways the girls’ subjectivities shift within the intra-actions.
Learning analytics stakeholders' expectations in higher education institutions: a literature review
Authors: Mahmoud Mai , Dafoulas Georgios , Abd ElAziz Rasha , Saleeb Noha 
Published: November 2020

PurposeThe objective of this paper is to present a comprehensive review of the literature on learning analytics (LA) stakeholders' expectations to reveal the status of ongoing research in this area and to highlight gaps in research.Design/methodology/approachConducting a literature review is a well-known method to establish knowledge and interpret the domain under examination (Snyder, 2019). In this paper, a descriptive approach was used to investigate the different research methods used to tackle LA stakeholders' expectations, which depends on summarising the studies' content and minimising the reviewer's interpretation.FindingsMost of the studies have used interviews and focus groups without testing isolated variables. Moreover, it was concluded that LA awareness and perception are affected by a lack of resources, technical skills, trustworthiness, data availability, accessibility, organisational culture, integration and lack of technology. 10;This paper sheds light on research on LA stakeholders' expectations and highlights the need for investigating developing countries' context in order to test the perception and impact of LA. The study has focussed mainly on papers published in journals, which may have led to missing some previous research papers.Originality/valueLiterature review studies provide an overview of the existing LA literature from general focus to analytical methods. As it is a broad topic, this paper focuses on reviewing studies about stakeholders, which will give a fuller understanding of how their expectations are being discussed beyond the currently assumed benefits of LA.
The impact of fair value estimates on audit fees: evidence from the financial sector in Jordan
Authors: Alqatamin Rateb Mohammad , Ezeani Ernest 
Published: November 2020

PurposeThis study investigates the association between the estimates of fair value and external auditor's fees.Design/methodology/approachBased on a sample of 32 Jordanian financial companies listed on the Amman Stock Exchange (ASE) over the period 2005–2018. We employ random effect models to test our hypothesis.FindingsWe found a positive relationship between audit fees and the proportion of fair value assets, which implies that external auditors are more likely to spend more effort for complex estimates, thereby increasing audit fees. We examined the relationship between audit fees and three levels of fair value inputs and found a positive relationship between the level of effort spent on assessment of higher uncertainty fair value inputs and audit fees. The findings are consistent with the expectation that more audit effort is required in a highly regulated environment due to the possibility of a higher cost of litigation.Practical implicationsThe findings of this study could be beneficial for a number of users of financial information, such as investors, regulators, auditors. This group of users might consider the results of this study when they are using a company's financial information, and consequently, better able to make the right decisions.Originality/valueAlthough prior studies have researched fair value, no study to date among developing countries has investigated its relationship with audit fees. This study, therefore, provides new empirical evidence that the complexity and risk of fair value estimates significantly influences auditors' motivation to expend additional effort, resulting in higher audit cost.
Academic incivility on job satisfaction and depressivity: can supervisory support be the antidote?
Authors: Abas Nurul Ain Hidayah , Lin Mei-Hua , Otto Kathleen , Idris Izazol , Ramayah T. 
Published: November 2020

PurposeAcademia is known for its high competitiveness, with prestige and diverse responsibilities and achievements being decisive determinants of success resulting in academic incivility. This paper extends Lazarus and Folkman's theory of stress by examining the moderating role of interpersonal justice (IJ) , as supervisory support, on academics' job satisfaction and depressivity.Design/methodology/approachThe study recruited 185 academics from a public university in Malaysia to participate in a survey. Using the partial least squares- structural equation modeling (PLS-SEM) analysis, academic incivility was negatively related to job satisfaction, whilst positively related to depressivity.FindingsAs hypothesized, it was found that the predicted detrimental effect of academic incivility on job satisfaction was buffered by perceiving high IJ from their immediate supervisors, i.e. deans or heads of department. An unanticipated finding was that there was a stronger relationship between academic incivility and depressivity for those academics who perceived high supervisory IJ.Practical implicationsFurther, academic management can formulate and revise zero-incivility policies and promote awareness explaining the detrimental impacts of incivility, despite support systems in academia.Originality/valueThis study provides the first empirical evidence showing the differential impact of supervisory IJ on two conditions of incivility–well-being relationships. Work culture and various sources of incivility should be considered for future research.
Cultural landscapes: exploring local people's understanding of cultural practices as “heritage”
Authors: Settimini Elena 
Published: November 2020

PurposeA vital testimony of human presence landscape is recognised and protected by international, national and local documents as an identity resource and one of the factors that contribute to the identity building processes (UNESCO, 1994; European Landscape Convention, 2000). The validation of landscape as cultural heritage presents not only new challenges but also opportunities for the heritage sector. In fact, a landscape plays a dual role: as part of the cultural heritage, which has to be preserved for its values, and as a “living” site, where individuals and groups live and work. This implies that the acknowledgement of its cultural significance should not be exclusively determined on the basis of discipline-driven frameworks and benchmarks but should rather be the result of a shared awareness within local communities.Design/methodology/approachThrough the analysis of the vineyard landscape of Langhe-Roero and Monferrato (Italy), the author discusses how the selection of a World Heritage site driven by “outstanding universal values” risks presenting a top-down approach to heritage processes.FindingsIn this article, the author explores how people living in this cultural landscape articulate their understandings of heritage values, and she addresses issues concerning their participation in decision-making processes, questioning whose values and meanings do the “outstanding universal value” legitimise or not.Originality/valueWhat the author argues is that the World Heritage listing's focus on extraordinary values risks constructing heritage around a consensus that privileges only some actors, whose voices and stories enliven the prestige of the wine production of this cultural landscape, omitting other values, memories and practices from the identity and meaning making processes. Does the identification and representation processes validated through World Heritage status capture how a landscape is understood by individuals and groups living within it? If not, how do these differences affect people's engagement? A further point of discussion is whether individuals and groups want to be engaged in decision-making processes and on which terms.
Physical appearance stereotypes for service occupations
Authors: Dean Dwane H. 
Published: November 2020

PurposeThis series of of five exploratory studies aims to investigate the idea that consumers have visual appearance expectations (stereotypes) for service providers in different occupations and how these visual stereotypes might affect provider choice.Design/methodology/approachSubjects were given color photos of white men and a list of service occupations and asked to infer the most likely occupation for each photo. A separate group of subjects reported the visual elements they most associated with the typical appearance of providers in various occupations. Other groups chose from a set of photos the depicted individual they would most want as their attorney or accountant.FindingsTwo studies confirmed that photo was a significant predictor of inferred service occupation, suggesting that consumers have visual appearance stereotypes for what a service provider should look like. A counter-intuitive finding was that the most stereotypical appearing service provider in an occupation was significantly less desired by subjects as their service provider than a less stereotypical appearing provider displaying a Duchenne (genuine) smile.Research limitations/implicationsOnly visual stereotypes of male service providers were explored, confined to the un-met provider condition. Also, the reported studies were exploratory, using small samples.Originality/valueThe results imply that impression formation and relationship marketing begin with the viewing of an un-met service provider’s photo by a potential client at a business website or social media. Further, the findings suggest that providers will want to display a Duchenne smile in their photo, as this visual element is favorably interpreted by consumers.
Multi-agency safeguarding arrangements: overcoming the challenges of Covid-19 measures
Authors: Driscoll Jennifer , Lorek Ann , Kinnear Elise , Hutchinson Aisha 
Published: November 2020

Purpose
Information sharing and joint working between agencies undertaking direct work with children have long been recognised as fundamental to robust and effective safeguarding and child protection arrangements. The public health response to Covid-19 disrupted those arrangements abruptly. This study aims to identify some of the innovative practices that have been implemented and how responses might inform planning for multi-agency working in the future.

Design/methodology/approach
This study presents reflections on preliminary fieldwork from a study of how agencies in London are responding to the challenges for multi-agency safeguarding arrangements created by the Covid-19 measures. It draws on the experience of expert practitioners in the research team as well as interviews with 17 senior professionals from local authorities, safeguarding partnerships and health.

Findings
The study participants endorsed known concerns around increased risks to children and raised new concerns about particular groups of children that under normal circumstances would not have been at risk. They identified some unexpected benefits derived from new arrangements, especially in relation to engagement with remote working.

Originality/value
Early insights are offered into promising initiatives to preserve strong multi-agency arrangements in crises and strengthen the resilience of the child protection system.
Moral re-armament: toward a better understanding of the society-corporation relationship before the emergence of “corporate social responsibility”
Authors: Dent Eric B. , Randall Craig 
Published: November 2020

Purpose
This study aims to introduce moral re-armament’s (MRA) role as a mediator in several labor/management disputes in industries primarily in the 1940s and 1950s. In this study, MRA was guided by a social responsibility framed in language that was a precursor to corporate social responsibility (CSR). This study features the case of the Miami-based airlines serving Latin America, who had experienced the longest airline labor strike to that date.

Design/methodology/approach
In this study, both artifacts and literary interpretations are used to inform an institutional-theory-based approach to a broad social movement, one player in that movement and its impact on an industry. Actions at one time can be shown to relate to activity and behavior at another time (Wadhwani and Bucheli, 2014). Thus, this paper has combined these perspectives in the approach to historically examine a precursor phenomenon of CSR.

Findings
MRA’s approach shared some methods, such as story-telling, with modern change management. This paper proposes that other methods that were important for that historical context played a significant role in MRA’s success. Today, these methods are no longer used. These include “intimacy” (MRA employees lived with members of labor and management while they were mediating), “theater” (they showed plays to all of those involved) and confessional sharing (their training was mostly a series of testimonies by those who previously were combative, but became collaborative when they accepted MRA’s principles).

Originality/value
This historical case may inspire those promoting CSR to expand their methods to have even greater success today.