bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Study of Icariin Modulating Hippocampal Neurogenesis in Depression Based on Quantitative CSF Proteomics
Authors: Zeng Ning-Xi , Li Huizhen , Wang Han-Zhang , Liu Kai-Ge , Gong Xia-Yu , Luo Wu-Long , Yan Can , Wu Li-Li 
Published: October 2020

Abstract
BackgroundHippocampal neurogenesis dysfunction is one of the main pathogenesis of depression. Icariin (ICA) has significant anti-depression and anti-hippocampal damage effects but could not effectively cross the blood-brain-barrier and accumulate in brain. Based on the proteomics of cerebrospinal fluid (CSF), this study aimed to explore the mechanism of ICA against hippocampal neurogenesis dysfunction in depression.MethodsIn vivo, rats were exposed to 6-week CUMS and treated by ICA to observe the effects of ICA on depressive symptoms, cognitive functions, neurogenesis and number of neurons in DG. Tandem mass tag (TMT) proteomics were used to screen the differentially expressed proteins (DEPs) in CSF co-regulated by CUMS and ICA. Parallel reaction monitoring (PRM) was used to validate 10 DEPs that were related to cell proliferation and survival. In vitro, CSF was conducted on primary hippocampal neural stem cells (NSCs) to observe the proliferation and differentiation under high-corticosterone (CORT) concentration.Results﻿It was shown that ICA could alleviate depressive symptoms, learning-memory dysfunction, neurogenesis dysfunction and neuronal loss in DG of depression rats and ICA-CSF could effectively repair the CORT-induced damage. A total of 52 DEPs co-regulated by CUMS and ICA in CSF were screened and mainly involved in ribosome pathway, PI3K-Akt pathway and IL-17 pathway. Rps4x, Rps12, Rps14, Rps19, Hsp90b1, Hsp90aa1 and HtrA1 were validated by PRM.ConclusionsThese findings indicate that to regulate the expression of proteins in CSF may be involved in the effects of ICA against hippocampal neurogenesis dysfunction in depression.
Was Lung Computed Tomography Necessary for Patients Receiving Selective Surgery From Low-risk Areas of COVID-19?
Authors: Fu Jun , Chen Zhida , Xu Xinghua , Pan Lichao , Gu Liangyou , Xu Chi , Du Yinqiao , Ni Ming , Chen Jiying , Chai Wei , Yu Qilin , Lu Qiang 
Published: October 2020

Abstract
ObjectiveLung computed tomography (CT) image was considered as supplementary diagnostic criteria for COVID-19 in the newest diagnosis and treatment program; however, the diagnostic effectiveness of lung CT in patients that have a strict screening for symptoms, history and reverse transcription-polymerase chain reaction (RT-PCR) testing remain unclear. To share our experience about elective surgery during the COVID-19 pandemic and to analyze the effectiveness and necessity of lung CT for screening COVID-19 in elective surgery patients from low risk areas.MethodsBased on the database of our Hospital Information System, participants were all patients receiving elective surgery in departments of general surgery, hepatological surgery, orthopedics, neurosurgery and urology at our hospital from 11 January, 2020 to 11 May, 2020.ResultsIn total, 2375 patients (1150 females and 1225 males) were enrolled in this current study. The mean age was 52 years old, ranging from 6 to 94. All the RT-PCR results of these 2375 patients were negative, including the patients with fever. The most common features on lung CT were nodular lesions (n=624, 26.3%) and striplike lesions (n=467, 19.7%). While, there were only 120 patients (5.1%) with ground-glass opacities (GGO) and 54 patients (2.3%) with lung consolidations on the lung CT, which were ruled out the COVID-19 by the RT-PCR results, clinical manifestation, fever screen, contact history and travel history. During the hospital stay, a total number of 1085 patients were screened with temperature ≥ 37.3℃, which were ruled out COVID-19 by consultation of special fever clinic and respiratory department.ConclusionsAfter strict screening for symptoms, history (contact COVID-19 patients or travelling to high-risk areas) and RT-PCR testing, lung CT image was not recommended as routine examination in patients receiving selective surgery from the low-risk areas of COVID-19.
Diagnosis of Brain Tumor Using Nano Segmentation and Advanced-cnn Classification
Authors: V DEEPA P , S Joseph Jawhar , Geisa Mary 
Published: October 2020

Abstract
Background: In recent days, the field of nano-technology is becoming popular due to increased rate of accurate detection and effectiveness in clinical patients using Computer-Aided Diagnosis (CAD). The high rate of precision with accuracy and classification of brain tumor as benign or malignant can be achieved with nano-scale imaging technology. This helps to enhance the superiority of life for brain tumor diseased patients. Results: In this work, we propose the novel Semantic nano-segmentation for the detection of brain tumors even in nano scale range. Proposed Semantic Nano-segmentation based on Advanced - Convolutional Neural Networks will help the radiologists to find the brain cancer even at early stages with nodules at very smaller size. The proposed method of Advanced - Convolutional Neural Networks (A-CNN) uses ResNet-50. Here the nano-image is taken as input and tumor image is segmented using Semantic Nano-segmentation which carries an average dice and SSIM values to be 0.2133 and 0.9704 respectively. The accuracy of 93.2% and 92.7% is obtained by the proposed Semantic nano segmentation for benign and malignant tumor images respectively. A-CNN method of automatic classification has an average accuracy of 99.57% and 95.7% for benign and malignant images respectively. Conclusion: This novel nano-method is created for effective detection of tumor area in nanometers (nm) and thus evaluates the disease perfectly. Closeness of the Proposed method at ROC curve with reference to True Positive values indicates higher performance than other methods. Comparative analysis on ResNet-50 with testing and training data at rate of 90% -10%, 80%-20% and 70%-30% respectively is made which proves the effectiveness of the proposed work.
A New and Convenient Method to Get Paraffin-Embedded Sections of Tuberculous Bacteria as Positive Quality Controls for Acid-Fast Staining
Authors: Tao Lili , Yu Guangyin , Huang Chuqiang , Tan Junliang , Yin Xiaomin , Yu Hao 
Published: October 2020

Abstract
Background: Acid-fast staining for the detection of Mycobacterium tuberculosis has a high false negative-rate, partly due to it is hard to get a good positive control tissue of acid-fast staining. We aimed to design a simple and convenient method for making positive quality controls for acid-fast staining in paraffin-embedded sections.Methods: Three methods were used to get more tuberculous bacteria, which involving centrifugation, mixing, and culture of tubercle bacilli in pleural fluid, prior to the preparation of paraffin-embedded sections.Results: Culturing tubercle bacilli in pleural fluid proved to be, by far, the best of the three methods, with sufficient bacteria, convenient observation, clear staining, and potential for upscaled production.Conclusions: The application of this method should improve the detection rate of tuberculous bacteria, thus facilitating clinical treatment.
Prognostic Influence of Tobacco Smoking on Human Papilloma Virus-Related Oropharyngeal Cancer is Dependent Upon Treatment Modality
Authors: ITAMI Jun , KOBAYASHI Kenya , MORI Taisuke , HONMA Yoshitaka , KUBO Yuko , MURAKAMI Naoya , OMURA Go , NAKAYAMA Yuko , IGAKI Hiroshi , OKUMA Kae , INABA Koji , TAKAHASHI Kana , KASHIHARA Tairo , KANEDA Tomoya , SHIMA Satoshi , TAKAHASHI Ayaka , SHIMIZU Yuri , YOSHIMOTO Seiichi 
Published: October 2020

Abstract
[Purpose] Tobacco smoking has been reported to influence the prognosis of human papilloma virus (HPV)-related orophageyngeal squamous cell carcinoma (OPSCC). However, it remains to be studied whether tobacco smoking equally affects the patients treated by various modalities. [Material and Method] From 2010 through 2018, 241 patients with OPSCC were treated in a single institution, out of which 144 patients had HPV-related OPSCC. P16 immunohistochemical staining was used as a surrogate of HPV infection. Two patients was excluded because of inadequate radiation dose, and the remaining　142 patients were the subject of this study. Median age was 63.8 years and more than 80% were male. More than 70% were smokers or ex-smokers with a median pack year of 17.3. Eighty-seven patients (61.3%) were classified as stage I.[Results] For all 142 patients with HPV-related OPSCC, overall survival (OS) and disease-specific survival (DSS) were 87.0% and 93.4% in 3 years, respectively. There were no differences of OS and DSS according to the stages by 8th edition of tumor, node, and metastasis (TNM) classification and the primary sites. OS and DSS were different by the amount of tobacco smoking expressed in pack year (PY) > 30 and < 30. Also the presence of secondary cancer impacted OS. However, the influence of the amount of tobacco smoking was reduced in the patients treated by radiation therapy. [Conclusions] The impact of tobacco smoking upon the prognosis of HPV-related OPSCC seems to be dependent upon therapeutic modalities.
Comparison of Two Inverse Planning Algorithms for Cervical Cancer Brachytherapy
Authors: Fu Qi , Xu Yingjie , Zuo Jing , An Jusheng , Huang Manni , Yang Xi , Chen Jiayun , Yan Hui , Dai Jianrong 
Published: October 2020

Abstract
Purpose: To compare two methods, the hybrid inverse planning optimization (HIPO) algorithm and the inverse planning simulated annealing (IPSA) algorithm, for cervical cancer brachytherapy and provide suggestions for their usage.Material and methods: This study consisted of 24 cervical cancer patients treated with CT image-based high-dose-rate brachytherapy using various combinations of tandem/ovoid applicator and interstitial needles. For fixed catheter configurations, plans were retrospectively optimized with two methods: IPSA and HIPO. The dosimetric parameters with respect to target coverage, localization of high dose volume (LHDV), conformal index (COIN), and sparing of organs at risk (OARs) were evaluated. A plan assessment method which combines a graphical analysis and a scoring index was used to compare the quality of two plans for each case. The characteristics of dwell time distributions of the two plans were also analyzed.Results: Both IPSA and HIPO can produce clinically acceptable treatment plans. The rectum D2cc was slightly lower for HIPO as compared to IPSA (p=0.002). All other dosimetric parameters for targets and OARs were not significantly different between the two algorithms. The generated radar plots and scores intuitively presented the plan properties and enabled to reflect the clinical priorities for the treatment plans. Different characteristics were observed between the dwell time distributions generated by IPSA and HIPO. Conclusions: Despite significant difference in dwell time distributions, both the two algorithms could generate high-quality treatment plans. With the help of the plan assessment method, the clinical decision on the optimal plan can be made quickly and consistently for each patient.
Successfully Treatment of Application Awake Extracorporeal Membrane Oxygenation in Critical COVID-19 Patient: A Case Report
Authors: Tang Junyi , Li Wencan , Jiang Fanli , Wang Tao 
Published: October 2020

Abstract
Background: A newly infectious diseases named coronavirus disease 2019 (COVID-19) emerged in China and now has spread in many countries, and constituted a public health emergency of international concern. Extracorporeal membrane oxygenation (ECMO) is used as salvage therapies in critical COVID-19 patients with respiratory/cardiac failure. Case presentation: A 49-year-old female patient was diagnosed with COVID-19 and progressed to critical cases, she was successfully treated with the application of awake extracorporeal membrane oxygenation. This case is the first reported successfully treatment of application awake ECMO in critical COVID-19 patient in China.Conclusions: Here we present the first reported successfully treatment of application awake ECMO in critical COVID-19 patient, however, whether awake ECMO can be widely used in the treatment of critical COVID-19 patients need more practice.
Fenofibrate Alleviates Insulin Resistance and Hepatic Steatosis via Modulation of let-7/ SERCA2b&nbsp;Axis
Authors: Zhang Dan , Niu Shan-zhuang , Ma Yi-cheng , Zhou Bo , Deng Yi , Geng Zuo-tao , Wen Yu , Luo Su-feng , Shi Chun-jing , Pu Guang-yu , Zou Cheng-gang , Chen Yuan-Li , Ma Lan-qing 
Published: October 2020

Abstract
Background: Fenofibrate is a peroxisome proliferator-activated receptor alpha agonist, which is widely used in clinical practice to effectively ameliorates the development of NAFLD. However, the molecular mechanism remains largely unknown, the present study aimed to investigate the role and specific mechanism of fenofibrate on lipid metabolism disorders associated diseases.Methods: The male C57BL6/J mice were divided into 3 groups, the mice in control group (n=10) were fed with normal chow diet, and the mice in HFD-fed group (n =10) were fed with a high fat diet (HFD) for 14 weeks. For the fenofibrate +HFD-fed group (n =10), the mice fed HFD were orally gavaged with fenofibrate (40 mg/kg) daily for the last 4 weeks. Body weight and hip width were measured. Macrosteatosis and fat deposition in the liver were measured by H&E staining and Oil red O staining individually. The levels of serum and hepatic triglyceride were measured, and HOMA-IR, HOMA-ISI were analyzed. The levels of SCD-1, Bip, CHOP and SERCA2b were measured by western blotting. The expression of let-7 were analyzed by qPCR, and the complementarity between the 3′-UTR of SERCA2b gene and let-7 was measured by luciferase reporter assay.Results: Fenofibrate reduces hepatic steatosis and insulin resistance in HFD-fed mice. Fnofibrate alleviates endoplasmic reticulum stress (ER stress) of mice fed a high fat diet (HFD). Fenofibrate increases the levels of Sarco-endoplasmic reticulum Ca2+-ATPase 2b (SERCA2b) which serves as a regulator of ER stress. Further, the levels of let-7 microRNA is also regulated by fenofibrate, and let-7 directly targets 3’-UTR of SERCA2b. Conclusion: The present data suggests that fenofibrate alleviates ER stress through the let-7/SERCA2b axis to protect against excessive lipid accumulation in the liver of Non-alcoholic fatty liver disease (NAFLD) mice.
Targeting STAT3 abrogates Tim-3 upregulation of adaptive resistance to PD-1 blockade on regulatory T cells of melanoma
Authors: Huang Lili , Xu Yu , Fang Juemin , Liu Weixing , Chen Jianhua , Liu Zhuqing , Xu Qing 
Published: October 2020

Abstract
Background Programmed cell death-1 (PD-1) blockade immunotherapies have demonstrated excellent clinical benefits in multiple cancers, but < 20% of melanoma patients respond to these treatments. Thus, it is crucial to understand for getting awareness of the dynamic changes vibrant transformations in the tumor microenvironment (TME) after PD-1 blockade, in exploring novel intervention targets as a potential therapy, for developing immunotherapy efficacy. MethodsTo ascertain the differentially expressed between PDCD1low and PDCD1high subsets and further PDCD1 related genes in skin cutaneous melanoma (SKCM) patients, a genomic analysis was conducted by The Cancer Genome Atlas (TCGA) datasets and web platform TIMER2.0 datasets. Pathway enrichment analysis was performed using the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway. Peripheral blood mononuclear cells (PBMCs), regulatory T (Treg) cells and B16F10 melanoma mouse were used as models. The cellular and molecular characteristics and mechanisms of Treg cells in melanoma were assessed by performing gene expression studies, immunohistochemistry, RNA sequencing, and flow cytometry.ResultsHere, we evaluate the countenance of T cell immunoglobulin and mucin-domain containing-3 (Tim-3), various immunosuppressive factors within tumor infiltrated regulatory T (Treg) cells after treated with anti-PD-1 or the indicator transduction and activator of transcription 3 (STAT3) inhibitors. Tim-3 gene is expressed differentially between PD-1low and PD-1high subsets from TCGA datasets and melanoma patients’ biopsies. Increased expression of Tim-3 is markedly observed within the tissues of the PD-1 blockade resistance of melanoma patients. Targeting STAT3 significantly boosts the response of resistant-PD-1 therapy within the melanoma mouse model. Mechanistically, the manifestation of STAT3 decreases the expression of Tim-3 and various cytokines in the purified Treg cells from individual peripheral blood mononuclear cells (PBMCs) and murine melanoma model, limiting the immunosuppression of Treg cells. ConclusionsOur findings indicate that Tim-3 expression on Treg cells within the TME is STAT3-dependent, providing support to STAT3 as a target and enhancing the immunotherapy for patients suffering from melanoma.
Development and Characterization of an Efficient Mutant Resource of the Mega Variety, Samba Mahsuri for Rice Improvement
Authors: Potupureddi Gopi , Balija Vishalakshi , Ballichatla Suneel , CG Gokulan , Awalellu Komal , Lekkala Swathi , Jallipalli Karteek , Mohammad Ershad , M Milton , Arutla Srikanth , Burka Rajender , Gouri Laha Shankar , Ayyangari Padmakumari Phani , Lella SubbaRao Venkata , Raman Sundaram Meenakshi , BC Viraktamath , Vemuri Ravindra Babu , Kranthi Brahma , Madnala Raju , Patel Hitendra Kumar , Sonti Ramesh Venkata , Madhav Maganti Sheshu 
Published: October 2020

Abstract
Background:Novel variants of rice (Oryza sativa L.) can be obtained from induced mutations. The objective of the present study is to induce mutations in the background of Samba Mahsuri (BPT-5204), a popular and mega rice variety of India for creating novel variants for morphological, physiological and biotic stresses through using Ethyl Methane Sulphonate (EMS).Results: A population derived from 10, 500 M1 plants and their descendants was phenotyped for a wide range of traits leading to the identification of 124 mutants having variations in key agro-morphological traits, and 106 mutants exhibited variation for physiological traits. Higher yield is the ultimate goal of crop improvement and we identified 574 mutants having higher yield compared to wild type by having better yield attributing traits. Further, a total of fifty mutants showed better panicle exsertion phenotypes as compared to Samba Mahsuri leading to enhancement of yield. Upon rigorous screening for three major biotic stresses, nine mutants showed enhanced tolerance for yellow stem borer, and thirteen different mutants each showed enhanced tolerance for sheath blight and bacterial leaf blight, respectively. On the whole, 1406 desired mutant lines identified at M2 were forwarded to an advanced generation (M5). Whole genome re-sequencing and analyses of 15 stable mutants revealed high level of similarities (99.91 to 99.99%) with the Samba Mahsuri. Conclusions:The lines showing enhanced tolerance to important biotic stresses (YSB, ShB and BLB) as well as several economically important traits are unique genetic resources which can be utilized for the identification of novel genes/alleles for different traits. The lines which have better agronomic features can be used as pre-breeding lines. The entire mutagenic population is maintained as a national resource for genetic improvement of the rice crop.
Effective Prevention of Atopic Dermatitis by Applying Fam’s Baby Moisturizer Twice Daily or Once Daily Compared with 2e Moisturizer Once Daily in High-Risk Neonates: Study Protocol for A Randomized, Blinded, Parallel, Three Group, Phase II Trial (PAF Study)
Authors: Inuzuka Yusuke , Yamamoto-Hanada Kiwako , Pak Kyongsun , Miyoshi Takekazu , Kobayashi Toru , Ohya Yukihiro 
Published: October 2020

Abstract
BackgroundAtopic dermatitis (AD) is a chronic and inflammatory skin disease that commonly affects young children. AD causes health-related burdens associated with pruritus and poor quality of life. The efficacy of moisturizer application shows inconsistency on primary prevention of AD according to previous randomized, controlled trials. Which moisturizer is effective and how often moisturizer should be applied to prevent development of AD in infants remain unclear. We hypothesize that twice daily application of moisturizer using Fam’s Baby is more effective than once daily application and that a sufficient amount of moisturizer application is effective for preventing development of AD in neonates and infants. Therefore, this study aimed to examine whether applying Fam’s Baby twice daily or once daily prevents AD compared with 2e moisturizer once daily in neonates and infants with at least one parent or sibling who has had atopic dermatitis.MethodsThis study is a single-center, three-parallel group, assessor-blind, superiority, individually randomized, controlled, phase II trial. A newborn with at least one parent or sibling who has had AD is randomly assigned to application of Fam’s Baby twice daily, Fam’s Baby once daily, or 2e once daily in a 1:1:1 ratio until 32 weeks old. The primary outcome is the time to the first onset of AD during administration of the moisturizer.DiscussionThis is the first phase II randomized, controlled trial in Japan to investigate whether applying moisturizer (Fam’s Baby) twice daily or once daily can prevent the incidence of AD compared with another moisturizer (2e) once daily in high-risk neonates. In this study, we will use 2e once daily as control moisturizer to confirm the efficacy for primary prevention of AD as found in our previous trial. Evaluation of application of moisturizers for preventing AD in this study is expected to contribute to a reduction in the prevalence of AD and a reduction in health care costs. Trial registrationJapan Registry of Clinical Trials (jRCT); ID: jRCTs031200070. Registered on 20 July 2020.
Daily Oral Low-Dose Vitamin A Supplementation Increases the Retinol Levels in Serum and Breast Milk of Lactating Mothers and Has No Effect on Infant Health Status: A Randomized Placebo-Controlled Trial in China
Authors: Ding Ye , Hu Ping , Yang Yue , Ye Mei , Lu Xiaolong , Jiang Wei , Xie Zhencheng , Fu Youjuan , Li Fang , Zhu Yunhua , Xu Fangping , Liu Ying , Wang Zhixu 
Published: October 2020

Abstract
Background: Vitamin A supplementation has been advocated as a potential strategy to improve the vitamin A status of lactating mothers and infants. In China, vitamin A supplements are readily available in the form of daily oral low doses. However, the existing clinical trials are limited to single or two high-dose maternal administration.Objective: We aimed to evaluate the effects of the daily oral low-dose vitamin A supplementation on the retinol levels in serum and breast milk of lactating mothers and the health status of infants in China.Methods: Lactating mothers who met the inclusion criteria and planned to continue exclusive breast-feeding were randomly assigned to receive either daily oral vitamin A and D drops (one soft capsule of 1800IU vitamin A and 600IU vitamin D2), or a matching placebo for 2 months. Before and after the intervention, the dietary intake was investigated by instant photography, and the retinol concentration in maternal serum and breast milk was determined by UPLC. During the trial, the health status of infants was diagnosed by pediatrician or reported by lactating mothers.Results: 245 participants completed the study with 117 in supplementation group and 128 in control group. After the 2-month intervention, maternal serum retinol concentration increased in supplementation group with no change in control group. Although breast milk retinol concentrations decreased significantly in both groups, the decrease in supplementation group was significantly less than that in the control group. However, maternal vitamin A supplementation was not associated with lower risks of infant febrile illness, respiratory tract infection, diarrhea and eczema respectively. Conclusion: Daily oral low-dose vitamin A supplementation is helpful to improve the maternal vitamin A status and can also play a positive role in vitamin A status of infants through breast milk.