bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development of the Other As Shamer scale Japanese version using Item Response Theory
Authors: Hiramatsu Yoichi , Asano Kenichi , Kotera Yasuhiro , Sensui Toshihiko , Endo Ayumu , Shimizu Eiji , Basran Jaskaran , Goss Ken 
Published: February 2020

Abstract
Objective; External shame is reflective of a person’s anxiety that he or she might be rejected by others. The Other As Shamer Scale (OAS) is a scale for assessing external shame. The Japanese version of OAS was developed, and its reliability and validity were examined using Item Response Theory (IRT). Results; A questionnaire survey was conducted with university students (N=199). Exploratory factor analysis of the results indicated a significantly high factor loading on the first factor, which was identical to the original version of the scale, as well as high internal consistency. Moreover, results confirmed that each item had adequate discrimination and information levels, suggesting that external shame could be discriminated with high accuracy with a wide range between relatively low and relatively high external shame groups. These results suggested that this scale could be used for screening external shame as a stress factor and for assessing intervention effects.
Altered Gut Microbiota in Infants is Associated with Respiratory Syncytial Virus Disease Severity
Authors: Harding Jeffrey , Siefker David , Vu Luan , You Dahui , Devincenzo John , Pierre Joseph , Cormier Stephania A 
Published: February 2020

Abstract
RationaleRespiratory syncytial virus (RSV) is the number one cause of lower respiratory tract infections in infants. There are still no vaccines or specific antiviral therapies against RSV, mainly due to the inadequate understanding of RSV pathogenesis. Recent data suggest a role for gut microbiota community structure in determining RSV disease severity.ObjectivesOur objective was to determine the gut microbial profile associated with severe RSV patients, which could be used to help identify at-risk patients and develop therapeutically protective microbial assemblages that may stimulate immuno-protection.MethodsWe enrolled 58 infants hospitalized with RSV, all were admitted to the pediatric ward. 53 remained in the pediatric ward (moderate) and 5 were moved to the pediatric intensive care unit at a later date (severe). We also enrolled 37 healthy infants during their “well-baby checkups” from 2012 to 2015. We evaluated the composition of gut microbiota from stool samples collected within 72 hours of enrollment via 16s sequencing of fecal DNA.Measurements and Main ResultsThere was a significant enrichment in S24_7, Clostridiales, Odoribacteraceae, Lactobacillaceae, and Actinomyces in RSV (moderate and severe) vs. controls. Patients with severe RSV disease had slightly lower alpha diversity (richness and evenness of the bacterial community) of the gut microbiota compared to patients with moderate RSV and healthy controls. Beta diversity (overall microbial composition) was significantly different between all RSV patients (moderate and severe) compared to controls and had significant microbial composition separating all three groups (control, moderate RSV, and severe RSV).ConclusionsCollectively, these data demonstrate that a unique gut microbial profile is associated with severe RSV disease prior to admission to the pediatric intensive care unit. More mechanistic experiments are needed to determine whether the differences observed in gut microbiota are the cause or consequences of severe RSV disease.
Study on Functional Monoclonal Antibodies of Anti-Human Uterine Sarcoma Stem Cell-Like CellsStudy on Functional Monoclonal Antibodies of Anti-Human Uterine Sarcoma Stem Cell-Like Cells
Authors: Gao Jiuping , Zhang Beilei , Tang Dihong , Gao Ting , Lin Qiuhui , Tang jun , Chu Chaonan , Yang Ting , Wang Jing , Cai Jingting , Wang Jing 
Published: February 2020

Abstract
Background: To establish a functional monoclonal antibody library using Human Uterine Sarcoma Stem Cell-Like Cells (HUSSLCs) to screen and identify functional monoclonal antibodies that can recognize and inhibit HUSSLCs.Methods: B lymphocytes in proliferative state were prepared by using the second generation CD133+spheroid cells of SK-UT-1 cell line, i.e. HUSSLCs, as antigens; Spheroid formation, agar colony formation, wound healing, flow cytometry, and Western blotting were adopted to detect the effect of monoclonal antibodies with varied dilution ratios on HUSSLCs spheroid formation, agar colony formation, cell migration, CD133 expression, and expression of CD44, ABCG2, Bmi1, Nanog, Oct4 and ALDH1.Results: Myeloma cells of SP2/0 cell line can achieve 85% degrees of fusion and results of 1-2F monoclonal cell supernatants with different dilution ratios reduced HUSSLCs spheroid formation rate, agar colony formation rate, cell migration rate, CD133 positive cell expression and protein expression levels of CD44, ABCG2, Bmi1, Nanog, Oct4, and ALDH1 in concentration-dependent manner (P <0.05).Conclusion: The antibody valence produced by HUSSLCs-immunized mice reached the requirement for preparation of monoclonal antibody. Anti-HUSSLCs monoclonal antibodies feature functions of inhibiting the self-renewal, unrestricted proliferation, migration, invasion and multidrug resistance of HUSSLCs and functions characterized by tumor stem cells.
Development and validation of bile acid profile-based scoring system for identification of biliary atresia: a prospective study
Authors: Zhao Dongying , Zhou Kejun , Chen Yan , Xie Wei , Zhang Yongjun 
Published: February 2020

Abstract
Background: Early distinguishing biliary atresia from other causes of infantile cholestasis remains a major challenge. We aimed to develop and validate a scoring system based on bile acid for identification of biliary atresia. Methods: In a prospective study, a total of 141 infants with cholestasis were enrolled in two sets (derivation cohort, n=66; validation cohort, n=75) from 2014 to 2018. Variables with significant difference between biliary atresia and non-biliary atresia infants were selected in the derivation cohort. Then, a scoring system including those variables was designed and validated. Results: Among 66 patients in the derivation cohort, 34 (51.5%) had biliary atresia. A scoring system was proposed with the following variables: glycochenodeoxycholic acid/chenodeoxycholic acid, clay stool, and gamma-glutamyl transferase. The total score ranged from 0 to 41, and a cutoff value of 15 identified biliary atresia with an area under receiver operating characteristic curve of 0.87 (95% confidence interval, 0.77–0.94), sensitivity of 85.3%, and specificity of 81.3% in the derivation cohort; these values were also confirmed in a validation cohort with a sensitivity of 90.0% and specificity of 80.0%. Conclusions: The proposed simple scoring system had good diagnostic accuracy for estimating the risk of biliary atresia in infants with cholestasis.
Correlates of Physical Activity Behavior in Adults: A Data Mining Approach
Authors: Farrahi Vahid , Niemelä Maisa , Kärmeniemi Mikko , Puhakka Soile , Kangas Maarit , Korpelainen Raija , Jämsä Timo 
Published: February 2020

Abstract
Purpose A data mining approach was applied to establish a multilevel hierarchy explaining physical activity (PA) behavior, and to methodologically identify the correlates of PA behavior. Methods The 46-year follow-up data from the population-based Northern Finland Birth Cohort 1966 were used to create a hierarchy using Chi-square Automatic Interaction Detection (CHAID) decision tree technique for predicting PA behavior. The study’s subjects were classified as physically active or physically inactive based on their activity profiles derived from objective measurement of PA. The variables were a wide list of potentially modifiable factors including self-reported, clinical, and environmental measures. We then analyzed the association of the factors emerging from the model with three PA metrics including sedentary (SED), light PA (LPA), and moderate-to-vigorous PA (MVPA) minutes per day. Results Model fitting was performed using a total of 168 factors as input variables to classify the PA behavior of 2,701 physically active and 1,881 physically inactive subjects. The decision tree selected a total of 36 factors of different domains by which 54 subgroups of subjects were formed. Factors emerging from the model were associated with the PA metrics, including body fat percentage (SED: B=26.5, LPA: B=-16.1, and MVPA: B=-11.7), normalized heart rate recovery 60 seconds after exercise (SED: B=-16.1, LPA: B=9.9, and MVPA: B=9.6), average weekday total sitting time (SED: B=34.1, LPA: B=-25.3, and MVPA: B=-5.8), and extravagance score (SED: B=6.3 and LPA: B=-3.7). Conclusions Using data mining, a data-driven model was established from empirical data that can be potentially utilized to identify subgroups for multilevel intervention allocation. An extensive set of factors was methodologically discovered that can be a basis for additional hypothesis testing in PA correlates research.
Does high occupational physical activity hamper the beneficial health effects of leisure time physical activity? Evidence of the physical activity health paradox from a prospective study on compositional accelerometry data and long-term sickness absence
Authors: Gupta Nidhi , Dencker-Larsen Sofie , Rasmussen Charlotte Lund , McGregor Duncan , Rasmussen Charlotte Diana Nørregaard , Thorsen Sannie Vester , Jørgensen Marie Birk , Chastin Sebastien , Holtermann Andreas 
Published: February 2020

Abstract
Background: The ‘physical activity health paradox’ advocates that leisure physical activity (PA) promotes health while occupational PA impairs health. However, this paradox can be explained by methodological limitations of the previous studies—self-reported PA measures, socioeconomic confounding or not addressing the compositional nature of PA. Therefore, this study investigated the association between compositions of accelerometer-based moderate to vigorous PA (MVPA) time at work and leisure and onset of long-term sickness absence (LTSA). Methods: Time spent on MVPA and remaining physical behaviours (sedentary behaviour, standing, light PA and time in bed) at work and leisure was measured for 929 workers using thigh accelerometry and expressed as isometric log-ratios (ilrs). LTSA was register-based events of ≥6 consecutive weeks during 4-year follow-up. The association between ilrs and LTSA was analysed using a Cox proportional hazards model adjusted for remaining physical behaviours and potential confounders, then separately adjusting for and stratifying on education and type of work. Results: During the follow-up, 21% workers experienced LTSA. During leisure, more relative MVPA time was negatively associated with LTSA (20% lower risk with 20 minutes higher MVPA, P=0.02). At work, more relative MVPA time was positively associated with LTSA (15% higher risk with 20 minutes higher MVPA, P=0.02). Beneficial association between MVPA at leisure and LTSA was only observed for the lowest tertile of MVPA at work (P=0.03). Results remained unchanged when adjusted for or stratified on education and type of work. Conclusion: These findings provide further support to the ‘PA health paradox’. Key words: physical activity, sedentary behavior, accelerometers, sick leave, occupational health, time-use epidemiology, register-based sickness absence
MEOX1 is a risk factor for unfavorable prognosis in human endometrioid endometrial adenocarcinoma
Authors: Liu Yunduo , Kong Dan , Liu Tianbo , Chen Xiuwei 
Published: February 2020

Abstract
Background: Mesenchyme homeobox 1(MEOX1) is a homeobox transcription factor. Although MEOX1 has an impact on cancer, no studies investigated its expression in human endometrioid endometrial adenocarcinoma (EEA). This study aim to check the expression level of MEOX1 and detect its role in unfavorable prognosis. Methods: We used Western blot analysis by fresh frozen tissues and Immunohistochemical (IHC) method to detect the protein levels of MEOX1. Results: MEOX1 expression at the protein levels in EEA was gradually higher than that in normal group and benign hyperplastic endometrium group (both P < 0.001). The high expression of MEOX1 was correlated with depth of myometrial invasion, lymfpho-vascular space involvement, FIGO stage，lymph node metastasis and histological grade by univariate survival analysis. MEOX1 high-expression status in EEA was an independent prognostic factor for recurrence-free survival (RFS) and overall survival (OS) by Multivariate survival analysis. Kaplan-Meier analysis demonstrated that MEOX1 expression was related to RFS and OS. Compared with the high expression MEOX1group,the low expression MEOX1 group had longer RFS and OS (both P < 0.001). Conclusion: MEOX1 may be a new potential biomarker for prognosis and recurrence in human EEA.
Impaired Dynamic Cerebral Autoregulation in Cerebral Venous Thrombosis
Authors: Chen Jie , Liu Jia , Dong Kehui , Wang Yilong , Zhao Xingquan , Wang Yongjun , Gong Xiping 
Published: February 2020

Abstract
Background: Cerebral autoregulation is crucial in traumatic brain injury, which might be used for determination the optimal intracranial pressure. Cerebral venous thrombosis is a cerebral vascular disease with the feature of high intracranial pressure, but the autoregulatory mechanism of which remains unknow. We aimed to investigate the capacity of cerebral autoregulation in patients with cerebral venous thrombosis. Methods: Twenty-three patients with cerebral venous thrombosis and 16 controls were enrolled. Cerebral autoregulation was assessed by transfer function analysis (rate of recovery / phase / gain) from spontaneous oscillations of cerebral blood flow velocity and arterial blood pressure. Results: A total of 76 middle cerebral arteries (MCAs) were investigated including 44 MCAs from CVT patients and 32 normal ones. Phase shift and rate of recovery estimated from CVT patients was significantly different from controls (37.63 ± 33.94 vs. 54.49 ± 26.44, p = 0.23; 29.74 ± 59.55 vs. 37.95 ± 22.55, p = 0.01, respectively). The bilateral impairment of cebral autoregulation was demonstrated, even in the hemispheres without severe sinus stenosis and brain parenchymal lesion. Conclusion: Our findings for the first time indicated that patients with cerebral venous thrombosis were more likely to have impaired cerebral autoregulation and suggest that cautious blood pressure control is required in such patients to prevent hyper or hypo-perfusion.
Energy efficiency in an integrated agro-ecosystem at the acid soil area in Mekong delta, Vietnam
Authors: Thao Nguyen Thi Thu , Tung Tra Van , Thao Nguyen Thi Phuong , Thanh Son Le , Braunegg Sibylle , Braunegg Gerhart , Schnitzer Hans , Le Hai Thanh 
Published: February 2020

Abstract
Background Both exergy and energy analysis methods are used for analysing energy efficiency in all kind of processes, and can be used also in agriculture. The study focuses on the connection of the three main process components (husbandry-crop-fishpond) in a typical farming household in an acid soil region in rural areas, Mekong delta, Vietnam. The concept of exergy analysis is used to underline the potential for energy efficiency in alternative processes in the agricultural system. To develop an integrated ecological system towards zero-emission, the analytical methods of material cycles and energy flows use a set of indicators of resource efficiency in a sustainable agriculture.Results The design of the ideal integrated farming system “Agro-Industrial Zero Emissions Systems” (AIZES) can increase the system efficiency by making use of indigenous natural materials and waste reuse, recycling. Recycling waste for energy, fish feed and fertilizing can result a decrease in half of environmental load. Using exergy analysis to calculate an indicator non-renewable yield ratio (NRYR), the systems imply sustainability of agriculture production. Conclusions The farming household will be able to replace fuel and electricity resulting in energy self-sufficiency to distribute surplus biogas to surrounding households. Biochar created by mixing the biomass residues with local plants can improve soil quality. Pig sludge can become nutritious fertilizer when mixed with biomass residues. Also, utilizing biogas can reduce purchased electricity.
Patterns of Local-regional Failures after Adjuvant Chemotherapy in Resected Pancreatic Cancer without adjuvant radiation therapy: Implication for Irradiation Volumes Design
Authors: Zhao Shengguang , Kirova Youlia , Qi Wei-Xiang , Chen Jiayi 
Published: February 2020

Abstract
In the present study, we aimed to assess the patterns of recurrence in pancreatic cancer patients, following surgical resection and adjuvant chemotherapy in the absence of adjuvant radiation , for optimal irradiation volumes design encompassing the majority of local recurrences. Isolated local regional recurrence (LRR) was defined as LR (local recurrence) and/or regional recurrence (RR) without distant metastasis (DM). The Fine and Gray competing risks regression analysis was performed to identified prognostic factors associated with isolated LRR. A total of 85 male and 29 female patients were included, 95 (83.3%) of the 114 patients had recurred. Most patients recurred at LRR (n=36, 37.9%), and isolated LR was seen in 23 patients(24.2%). The median time between the end of the treatment and initial recurrence was 9.8 months (2.2-71). Competing risks analysis demonstrated that surgery combined with vascular resection (HR 2.44, 95% CI: 1.40–4.27, p =0.002) and R1 resection status (HR 3.96, 95% CI: 2.23-7.02, p <0.0001) significantly increased the risk of developing isolated LRR. The most common site of specific LRR were observed in the lymph node regions (LN) No. 14p (30.5%), followed by pancreas or tumor bed (23.7%), LN No.16b1 (23.7%), celiac artery (22%), and superior mesenteric artery (22%). In conclusion, the risk of developing isolated LRR in patients with PDAC who underwent curative resection and adjuvant treatment remains high, especially for patients received surgery combined with vascular resection or R1 resection. Adjuvant radiotherapy for tumor bed/vessels and involved regional nodes including partially the region No.16 is recommended for this patient population.
Realist Evaluation of Public Engagement and Involvement in Data-intensive Health Research
Authors: Hobbs Georgina , Tully Mary Patricia 
Published: February 2020

Abstract
Background High quality public engagement and involvement (PEI) in data-intensive health research is seen as one way of ensuring that social legitimacy, i.e. a social license, is conferred through public acceptance of the need for research use of their data. This is a complex research area, and portfolios of involvement have been suggested, but not yet evaluated, to support the role of public contributors. The study aim was to evaluate if and how membership of a data-intensive research public forum that included the use of an involvement portfolio would act as a mechanism for enhancing members’ personal development. Our preliminary middle range theory was that the use of a portfolio as a framework for learning would facilitate forum members’ personal development. Method Qualitative data were collected from 15 current and previous forum members, via semi-structured interviews, notes from meetings, and consultations with and feedback from members. Data were critically compared, contrasted and reviewed until no new themes could be discerned and then condensed into context-mechanism-outcome (CMO) configurations. Realist evaluation was used to generate a theoretical and empirical appreciation of the contextual circumstances and mechanisms which help to explain the extent to which involvement with a public forum would enhance members’ personal development and, if so, how, why, and for whom. Results Three CMO configurations were identified. All of them showed that using the portfolio facilitated growth in forum members’ personal development, but only where the members valued using the portfolio. This was particularly so for female forum members. Members valued the portfolio in one or more of three ways: as a tool to record and evidence activities, to facilitate reflective practice or as a guiding framework. Conclusions Data analysis and consideration of the three CMO configurations suggests a refined middle range theory that ‘The use of a portfolio as a framework for learning in a public forum will facilitate forum members’ personal development if they value its use as a framework for learning’. Further work is needed to confirm these findings both elsewhere in data-intensive health research and in other complex research areas using public forums for PEI.
Investigating the effect of Ultra-low dose naloxone on spinal BDNF and KCC2 cotransporter in morphine tolerant and hyperalgesia rats
Authors: Baratzadeh Mozhgan , Danialy Samira , Zaringhalam Jalal , MANAHEJI HOMA 
Published: February 2020

Abstract
Background: Chronic opioids administration could lead to several side effects including morphine tolerance and opioid induced hyperalgesia (OIH). Tolerance and hyperalgesia to opiates reduce their effectiveness in the treatment of severe pain. Although the mechanisms are unclear. Recently, we have shown that repeated morphine treatments induced increases in spinal PKCᵧ and GAT-1 expression. In this study we investigate the BDNF and KCC2 expression through ultra-low dose of naloxone coadministration of morphine.Results: In morphine group, rats received 10 mg i.p. morphine and in treatment group 15ng ultra-low dose of naloxone with morphine for consecutive 8 days. Behavioral tests were performed on day 1 before and after the morphine injection, day 5, 8 (tolerance test) and 10, 48h after last morphine injection (opioid induced hyperalgesia OIH test). A number of rats were sacrificed on day 8 and others on day 10, then expression of BDNF and KCC2 were analyzed by western blot and immunohistochemistry techniques, respectively. Behavioral tests suggested that following 8 days of chronic morphine injection tolerance developed. OIH was shown 48 hours after the last morphine injection. Expression of BDNF significantly was increased and KCC2 downregulated in rats that developed morphine tolerance and OIH respectively. Ultra-low dose of naloxone by decreasing BDNF and increasing KCC2 was able to suppress development of OIH and alleviated morphine tolerance.Conclusions: Our data suggest that BDNF and KCC2 maybe candidate molecules which are involved in tolerance and OIH. Ultra- low dose of naloxone along morphine might be a valuable therapeutic potential for controlling hypersensitivity following chronic morphine administration.
Sexual-biased gene expression of olfactory-related genes in the antennae of Conogethes pinicolalis (Lepidoptera: Crambidae)
Authors: Jing Dapeng , Zhang Tiantao , Bai Shuxiong , He Kanglai , Prabu Sivaprasath , Luan Junbo , Wang Zhenying 
Published: February 2020

Abstract
Background: Conogethes pinicolalis (Lepidoptera: Crambidae), is similar to Conogethes punctiferalis (yellow peach moth) and its host plant is gymnosperms , especially for masson pine. So far, less literature was reported on this pest. In the present study, we sequenced and characterized the antennal transcriptomes of male and female C. pinicolalis for the first time.
Results: Totally, 26 odorant-binding protein (OBP) genes, 19 chemosensory protein (CSP) genes, 55 odorant receptor (OR) genes and 20 ionotropic receptor (IR) genes were identified from the C. pinicolalis antennae transcriptome and amino sequences were annotated against homologs of C. punctiferalis . The neighbor-joining tree indicated that the amino acid sequence of olfactory related genes is highly homologous with C. punctiferalis . Furthermore, the reference genes were selected, and we recommended the phosphate dehydrogenase gene (GAPDH) or ribosomal protein 49 gene (RP49) to verify the target gene expression during larval development stages and RP49 or ribosomal protein L13 gene (RPL13) for adult tissues.
Conclusions: Our study provides a starting point on the molecular level characterization between C. pinicolalis and C. punctiferalis , which might be supportive for pest management studies in future.