bluuebunny/crossref_metadata_2025_split huggingface dataset:
CTCF, a novel fusion partner of ETO2 in a post-transplant relapsed acute myeloid leukemia patient
Authors: Li Jiao , Shen Zhen , wang zheng , Xu Yi , Zeng Zhao , Bian Xiaosen , Zhang Jun , Pan Jinlan , Wu Wenzhong , Yao Li , Chen Suning , Wen Lijun 
Published: July 2020

Abstract
Background

ETO2 is a nuclear co-repressor, which plays a critical role in the regulation of the cell cycle, self-renewal capacity, and differentiation of hematopoietic progenitor cells.
Methods

We identified novel fusion transcripts involving ETO2 and CTCF by RNA-seq in a post-transplant relapsed case.
Results

The CTCF-ETO2 and ETO2-CTCF chimeric genes were validated by RT-PCR and Sanger sequencing. In addition, both transcripts apparently promoted cell proliferation which is beneficial to tumorigenesis.
Conclusion

The novel fusions may have prognostic value and pathogenic mechanisms in acute myeloid leukemia.
Simultaneous Targeting Tumor Cells and Cancer-Associated Fibroblasts with a Paclitaxel-Hyaluronan Bioconjugate: in vitro Evaluation in Non-Melanoma Skin Cancer
Authors: Bellei Barbara , Caputo Silvia , Migliano Emilia , Lopez Gianluca , Marcaccini Valeria , Cota Carlo , Picardo Mauro 
Published: July 2020

Abstract
Background: Cancers are complex organs which encompass not only the tumor cells but also cells within the surrounding stroma. Such cells, mainly cancer associated fibroblasts (CAFs) and immune infiltrating cells, facilitate many aspects of cancer development providing a structural and supportive framework rich of bioactive compounds. So far, there are emerging studies proposing the combination of conventional anti-cancer therapy, directed against neoplastic cells, to molecules targeting tumor microenviroment. Methods: The study was designed to evaluated the pharmacological properties of the anti-tumor agent paclitaxel conjugated to hyaluronic acid (HA) on non-melanoma skin cancer (NMSC) and on the surronding dermal fibroblasts. This molecule, named Oncofid-P20 (Onco-P20), targets preferentially cells expressing high level of CD44, the natural ligand of HA.Results: Consistent with paclitaxel’s mechanism of action involving interferences with the normal breackdown of microtubules during cell division, highly sensible carcinoma cells underwent rapidly to apoptotic cell death. Interestingly, less sensible cells such as dermal fibroblasts resisted to the Onco-P20 treatment and experirenced a senescent-like status characterized by morphological change, decreaded proliferation, and significant modification of the gene espression profile. Onco-P20-treated fibroblasts lowered growth factors production, down-modulate Wnt signal pathway and acquired a pro-inflammatory profile. Independently to the direct exposure to taxol, in presence of Onco-P20-treated fibroblasts or their conditioned medium, carcinoma cells reduced the proliferation rate. Similar to NHF, fibroblasts isolated from skin cancer tumor lesion or from tissue adjacent to the tumor acquired an anti-neoplastic activity under Oncofid-P20 treatment.Conclusion: Collectively, our data demonstrated that Onco-P20, exerting both a direct and a NHF-mediated indirect paracrine effect on carcinoma cells, is a good candidate for an innovative therapy alternative to surgery for treatment of NMSC.
lncRNA APOC1P1-3 promoting anoikis-resistance of breast cancer cells
Authors: Lu Qi , Wang Li , Zhu Ping , Li Luying , Wang Xue , Jin Youping , Zhi Xiuling , Yu Jerry , Li Xin , Qin Xingjun , Zhou Ping 
Published: July 2020

Abstract
Background

Anoikis resistance plays a critical role in the tumor metastasis by allowing survival of cancer cells in the systemic circulation. We previously showed that long non-coding RNAs APOC1P1-3 (lncRNA APOC1P1-3) inhibits breast cancer cell apoptosis. However, its role in the anoikis resistance remains unclear.
Methods

We induced anoikis resistance in two breast cancer cell lines (MCF-7 and MDA-MB-231) under anchorage-independent culture condition and studied the effects of lncRNA APOC1P1-3 on the apoptosis. The Dual-Luciferase activity assay were used to whether miRNA-188-3P can specifically bind to lncRNA APOC1P1-3. We further explored the role of APOC1P1-3 in the lung metastasis by injecting MDA-MB-231 and MDA-MB-231-APOC1P1-3-knock-down cells in the female BALB/c nude mice.
Results

We found that it suppressed early apoptosis of these cells by gain or loss of their function, respectively. We further explored its mechanism related to anoikis resistance, and found this molecule promoted the resistance via activating Caspase 3, 8, 9 and PARP. Moreover, it was specifically binding to the target miRNA-188-3p to block its inhibition of Bcl-2 (an anti-apoptosis protein). These findings suggest that lncRNA APOC1P1-3 plays an important role in the development of breast cancer metastasis via anoikis resistance.
Conclusions

This study demonstrates that lncRNA APOC1P1-3 can promote the anoikis resistance of breast cancer cells and specifically bind to miRNA-188-3p acting as a “sponge” to block the inhibition of anti-apoptotic protein Bcl-2.
Did the COVID-19 Pandemic Have Immediate Impacts on the Socio-Emotional and Digital Skills of Japanese Children
Authors: Moriguchi Yusuke , Sakata Chifumi , Meng Xianwei , Todo Naoya 
Published: July 2020

Abstract
Background: A novel coronavirus, SARS-CoV-2, has spread widely throughout the world. To reduce the spread of infection, children are prevented from going to school and have fewer opportunities for in-person communication. Although the pandemic has impacted the everyday lives of children, its impact on their development is unknown. This cross-sectional study compared Japanese children’s socio-emotional behaviors and skills for operating digital devices before and during the pandemic.Methods: Parents completed a web-based questionnaire before and during the pandemic for children ages 0-9. Children’s socio-emotional development in an everyday context was assessed using the Strengths and Difficulties Questionnaire (SDQ). Children’s basic touch interaction skills to operate digital devices and skills to use functions of digital devices were also measured.Results: The results indicated that during the pandemic, children were more prosocial, experienced more problems in their peer relationships, and had better digital skills, but no differences were found in emotional symptoms, conduct problems, hyperactivity between before and during the pandemic. The differences in digital skills was explained by the duration of children’s media use.Conclusions: Overall, our results suggest the pandemic may have immediate impact on children’s socio-emotional behaviors and digital skills. Trial registration: We pre-registered our hypotheses, method, primary analyses, and sample size (https://osf.io/c7p6b)
Preoperative False-Negative Transthoracic Echocardiographic Results in Native Valve Infective Endocarditis Patients: A Retrospective Study from 2001 To 2018
Authors: Ren Zuning , Zhang Jian , Chen Hongjie , Mo Xichao , Cai Shaohang , Peng Jie 
Published: July 2020

Abstract
Background: Infective endocarditis (IE) is a lethal disease that is difficult to diagnosis early. Although echocardiography is one of the most widely used diagnostic technique, it has limited sensitivity. This study surveyed the clinical features of IE patients who underwent surgery and compared transthoracic echocardiography and histological findings to explore the factors related to false-negative echocardiographic results.Methods: Medical records were extracted from IE patients consecutively hospitalized between June 2001 and June 2018. Results: A total of 182 patients with native valve IE who underwent surgery were included. Compared to the non-surgery group, the surgery group was more likely to have pre-existing valvular lesions and more serious cardiac conditions and a relative lack of signs of infection and cerebrovascular events, leading to a lower proportion of “definite cases” before surgery. The false-negative rate of echocardiography was 14.5%. Echocardiography has significant disadvantages in diagnosing perivalvular abscesses, valve perforations, and left-sided endocarditis, especially for subjects with both aortic and mitral valve infections. The multivariate analysis identified congenital heart disease and small vegetations (<10 mm) as independent predictors of false-negative echocardiography results. Conversely, fever and heart murmurs on admission served as protective factors. Conclusions: Under some circumstances, echocardiography provides inconsistent results compared with surgical findings, and negative echocardiography results do not rule out IE. The diagnosis of IE depends on comprehensive evaluations using multiple methods.
Predicting adverse events in patients who are anxious about dental procedures
Authors: Oike Yasunobu , Takamura Norito , Tokunaga Jin , Ogata Kenji , Setoguchi Nao , Nakamura Mariko 
Published: July 2020

Abstract
Background

Dental extractions can cause significant anxiety making patients feel sick. In serious cases, extractions can cause unconsciousness and even death. Therefore, systemic adverse events must be completely avoided. We herein developed a method to predict dental extraction-related adverse events in patients.
Methods

The State-Trait Anxiety Inventory (STAIY-1) was administered before calculus removal and extraction, and STAIY-2 was administered before calculus removal in 93 patients (men, 44; women, 49). These patients were at a dental clinic for outpatient treatment. The vital signs like blood pressure, pulse rate, and arterial oxygen saturation and salivary amylase activity were measured at 2- and 4-min intervals before calculus removal as well as before extraction.
Results

Of the 93 patients, one man and one woman suffered from an adverse event. Maximum points for raw STAIY-1 scores were measured before extraction for both men and women, suggesting that performing the STAIY-1 survey prior to extraction is effective in predicting extraction-related adverse events. We also investigated rate changes (rate change = mean value during extraction − mean value during calculus removal/mean value during calculus removal) and mean pre-extraction pulse rates in patients who suffered from an adverse event. Adverse events occurred in men when there was a rate change of ≥ 0.225 and mean pre-extraction pulse rate was ≥ 90.0 bpm and in women when there was a rate change of ≥ 0.200 and the mean pre-extraction pulse rate was ≥ 95.0 bpm. We used these pulse rates as cutoff values.
Conclusions

Thus, the combined use of pre-extraction STAIY-1 results and pulse rate cutoff values could increase the success of predicting adverse events in such patients.
Zadek osteotomy for the treatment of Haglund’s Syndrome
Authors: Zheng Weixin , Du Junhua , Liang Jingqi , Zhang Yan , Liang Xiaojun , Zhao Hongmou 
Published: July 2020

Abstract
Background: Although several treatment options described in paper, Haglund’s Syndrome (HS) is a challenging common lower extremity disorder. This study reported the clinical outcomes of Zadek osteotomy (ZO) for the treatment of HS.Methods: This retrospective study included 19 patients who underwent ZO from January 2016 to June 2018. Patients were evaluated using the American Orthopedic Foot Ankle Society ankle-hindfoot scale (AOFAS), Visual Analogue Scale (VAS), ankle range of motion (AROM), and Chauveaux-Liet (CL) angle preoperatively and at final follow-up visit (16.3±4.2) months. Postoperative complications of patients were also recorded.Results: The ZO exhibited a significant improvement (P＜0.05) in preoperative to postoperative AOFAS (from 53.2±5.4 to 86.4±6.5), VAS ( from 6.3±2.1 to 0.9±1.2 ) scores and CL angle (from 18.2±1.3 to 9.4±2.1 ) degrees, the dorsiflexion of AROM (DAROM) (from 23.4±2.3 to 29.4±1.2) degrees. The planter flexion of AROM (PAROM) (from 32.5±3.5 to 33.2±3.4) degrees (P＞0.05). One postoperative complication was observed: a case of hardware pain in healthy patient. The overall rate of satisfaction after surgery was (94.7%).Conclusions: ZO is effective and safe procedure for the treatment of HS. This method can be a good option for those patients of HS.
3D Simulation of Radiographic Projections to Test and Reduce the Effect of Pelvic Tilt on the Accuracy of Cross-table Lateral Radiography
Authors: Xu Jie , Su Baohua , Zhang Wenhui , Sun Hao , Li Deng , Cai Zhiqing , Chen Meiyi , Qiu Meiling , Ma Ruofan 
Published: July 2020

Abstract
Background:Cross-table lateral (CL) radiography is aconvenientandfeasible method toassess cup version angle (VA) after total hip arthroplasty; however, the pelvic tilt (PT) may contribute to its measurement inaccuracy.We sought (1) to determinethe effect of the PT on cup version measurement on CL radiography and (2) to develop a method for reducing measurement errors caused by the PT.Methods: Two groups of VAswere measured respectively on 3D simulatedCL images and CL radiographs. Based on the data, we created a corrective formula to convert the radiographic measurements into values approximating the actual VA under a natural pelvic position. The corrected valueswere compared with computed tomography (CT) measurements to verified the corrective formula. Results:There was a high correlation between the measurements and PTs. For each 1-degree increased in the PT, the VA decreased by 0.76° (R²=0.995, p<0.001). The VA measurements corrected by our equation was in high agreement with the CT-measured valueswithreferenceto the corresponding PT (intra-class correlation coefficient [ICC]=0.988, p<0.001), which was in sharp contrast to that without PT control (ICC=0.454, p=0.203).Conclusions: ThePT may contribute to cup version measurement inaccuracies on CL radiography. Our mathematical algorithm can serve as a reliable method to improve the accuracy of CL radiography.
Nanopolystyrene Translocation and Fetal Deposition After Acute Lung Exposure During Late-Stage Pregnancy
Authors: Fournier Sara B , D’Errico Jeanine N , Adler Derek S , Kollontzi Stamatina , Goedken Michael J , Fabris Laura , Yurkow Edward J , Stapleton Phoebe A 
Published: July 2020

Abstract
Background: Plastic is everywhere. It is used in food packaging, storage containers, electronics, furniture, clothing, and common single-use disposable items. Microplastic and nanoplastic particulates are formed from bulk fragmentation and disintegration of plastic pollution. Plastic particulates have recently been detected in indoor air and remote atmospheric fallout. Due to their small size, microplastic and nanoplastic particulate in the atmosphere can be inhaled and may pose a risk for human health, specifically in susceptible populations. When inhaled, nanosized particles have been shown to translocate across pulmonary cell barriers to secondary organs, including the placenta. However, the potential for maternal-to-fetal translocation of nanosized-plastic particles and the impact of nanoplastic deposition and accumulation on fetal health remain unknown. In this study we investigated whether nanopolystyrene particles can cross the placental barrier and deposit in fetal tissues after maternal pulmonary exposure.Results: Pregnant Sprague Dawley rats were exposed to 20 nm rhodamine-labeled nanopolystyrene beads (2.64 x 1014 particles) via intratracheal instillation on gestational day (GD) 19. Twenty-four hours later on GD 20, maternal and fetal tissues were evaluated using fluorescent optical imaging. Fetal tissues were fixed for particle visualization with hyperspectral microscopy. Using isolated placental perfusion, a known concentration of nanopolystyrene was injected into the uterine artery. Maternal and fetal effluents were collected for 180 minutes and assessed for polystyrene particle concentration. Twenty-four hours after maternal exposure, fetal and placental weights were significantly lower (7% and 8%, respectively) compared with controls. Nanopolystyrene particles were detected in the maternal lung, heart, and spleen. Polystyrene nanoparticles were also observed in the placenta, fetal liver, lungs, heart, kidney, and brain suggesting maternal lung-to-fetal tissue nanoparticle translocation in late stage pregnancy.Conclusion: These studies confirm that maternal pulmonary exposure to nanopolystyrene results in the translocation of plastic particles to placental and fetal tissues and renders the fetoplacental unit vulnerable to adverse effects. These data are vital to the understanding of plastic particulate toxicology and the developmental origins of health and disease.
Effects of chronic kidney disease on mid- and long-term clinical outcomes after off-pump coronary artery bypass grafting-a retrospective study
Authors: Li Xihui , Song Bo , Dong Shiyong , Zhang Siyu 
Published: July 2020

Abstract
Backgroud: To investigate the effect of chronic kidney disease (CKD) on mid- and long-term clinical outcomes after off-pump coronary artery bypass grafting (CABG).Methods: This was a retrospective analysis of data of 1141 patients discharged from the Department of Cardiac Surgery, Peking University First Hospital from January 2010 to June 2018 after undergoing off-pump CABG. Preoperative baseline, operative, and follow-up data obtained at regular outpatient visits or by telephone calls were collected. Follow-up endpoints included stroke, nonfatal myocardial infarction, heart failure, revascularization, and all-cause death. Patients with preoperative estimated glomerular filtration rate calculated, using the Chronic Kidney Disease Epidemiology equation of≥ 60 mL/min/1.73 m2 and < 60 mL/min/1.73m2, were assigned to normal Group (1, 910 cases) and CKD group (231 cases), respectively. The effects of CKD on selected endpoint events were compared and analyzed.Results: There was a higher proportion of women, more preoperative complications, and a higher incidence of early postoperative complications in patients with CKD than in those with normal renal function. After 1–9 years of follow-up (mean5.0±2.2 years), the incidences of stroke, non-fatal myocardial infarction, and all-cause mortality were significantly higher in the CKD than in the normal renal function group, whereas incidences of revascularization and heart failure were not. Logistic regression analysis showed that preoperative CKD was a risk factor for stroke, non-fatal myocardial infarction, and all-cause death during follow-up. After correcting for common confounding factors, such as sex, age, and left ventricular ejection fraction, preoperative CKD was a risk factor for non-fatal myocardial infarction (OR 2.675, 95% CI 1.023–6.995, P=0.045) and all-cause death (OR 1.833, 95% CI 1.079–3.114, P=0.025).Conclusions: In patients undergoing off-pump CABG, preoperative CKD is associated with increases in the incidences of mid- and long-term non-fatal myocardial infarction and all-cause mortality.
Detection and prevalence of depression among adult type 2 diabetes mellitus patients attending non-communicable diseases clinics in Lilongwe, Malawi
Authors: Udedi Michael , Pence Brian W. , Stewart Robert C. , Muula Adamson S. 
Published: July 2020

Abstract
Background
Depression is associated with chronic physical illnesses and negatively affects health outcomes. However, it often goes undiagnosed and untreated. We investigated the prevalence of depression among adult type 2 diabetes mellitus (T2DM) patients attending non-communicable diseases (NCD) clinics in Lilongwe, Malawi, and estimated the level of routine detection by NCD clinicians. This study set out to determine the prevalence of major depression and its detection among adult type 2 diabetes mellitus (T2DM) patients attending NCD clinics in Lilongwe, Malawi.
Methods
In a cross-sectional study design, 323 T2DM patients aged ≥ 18 years were screened for depression with the Patient Health Questionnare-9 (PHQ-9) followed by diagnostic assessment with the Structured Clinical Interview for DSM-IV (SCID). We analysed the association between presence of major depression and sociodemographic factors using logistic regression.
Results
Three quarters of the participants (76%) were females. The participants’ ages ranged from 21–79 years. Of the 323 participants, 58 (18%) met criteria for DSM-IV major depression. None of the cases of major depression had been identified by the NCD clinicians. Major depression was found not to be significantly associated with any of the sociodemographic factors.
Conclusions
We found that depression is common among NCD clinic attendees with T2DM in Malawi,, and poorly detected by NCD clinicians. Given the high prevalence and challenges in clinical identification, integration of depression screening with a standardized validated tool should be a high priority so as to link patients to appropriate services.
Immediate Bystander Response to Sudden Cardiac Arrest During Sports is Associated with Improved Survival -- A Video Analysis.
Authors: Panhuyzen-Goedkoop Nicole M , Wellens Hein JJ , Verbeek Andre LM , Piek Jan J , Peters Ron JG 
Published: July 2020

Abstract
Background Sudden cardiac arrest (SCA) during sports can be the first symptom of yet undetected cardiovascular conditions. Immediate chest compressions and early defibrillation offer SCA victims the best chance of survival, which requires prompt bystander response.Aims To determine the effect of rapid bystander response to SCA during sports by searching for and analyzing videos of these SCA/SCD events from the internet.Methods We searched images.google.com, video.google.com and YouTube.com, and included any camera-witnessed non-traumatic SCA in athletes and other sports participants at any sports facility. The rapidity of starting bystander chest compressions and defibrillation was classified as <3, 3-5, or >5 minutes. The year SCA occurred was allocated to 1990-2009, 2010-2014 or 2015 onwards, compatible with the current guidelines.ResultsWe identified and included 28 victims of average age 27.9 years (SD=9.8); 27 were males, 22 elite athletes, and 17 participated in soccer. Bystander response <3 minutes (6/28) or 3-5 minutes (1/28) and defibrillation <3 minutes was associated with 100% survival. Not performing chest compressions and defibrillation was associated with death (14/28), and >5 minutes delay of intervention with worse outcome (death 4/28, severe neurologic dysfunction 1/28). Survival was highest between 2010-2014 (71.4%).ConclusionsAnalysis of internet videos showed that immediate bystander response to non-traumatic SCA during sports was associated with improved survival. This suggests that immediate chest compressions and early defibrillation are crucially important in SCA during sport, as they are in other settings. Optimal use of both will most likely result in survival. The observed bystander responses to SCA during sports do not show awareness of current guidelines.