bluuebunny/crossref_metadata_2025_split huggingface dataset:
The proteomic analysis of breast cell line exosomes reveals disease patterns and potential biomarkers
Authors: Risha Yousef , Minic Zoran , Ghobadloo Shahrokh M. , Berezovski Maxim V. 
Published: August 2020

AbstractCancer cells release small extracellular vesicles, exosomes, that have been shown to contribute to various aspects of cancer development and progression. Differential analysis of exosomal proteomes from cancerous and non-tumorigenic breast cell lines can provide valuable information related to breast cancer progression and metastasis. Moreover, such a comparison can be explored to find potentially new protein biomarkers for early disease detection. In this study, exosomal proteomes of MDA-MB-231, a metastatic breast cancer cell line, and MCF-10A, a non-cancerous epithelial breast cell line, were identified by nano-liquid chromatography coupled to tandem mass spectrometry. We also tested three exosomes isolation methods (ExoQuick, Ultracentrifugation (UC), and Ultrafiltration–Ultracentrifugation) and detergents (n-dodecyl β-d-maltoside, Triton X-100, and Digitonin) for solubilization of exosomal proteins and enhanced detection by mass spectrometry. A total of 1,107 exosomal proteins were identified in both cell lines, 726 of which were unique to the MDA-MB-231 breast cancer cell line. Among them, 87 proteins were predicted to be relevant to breast cancer and 16 proteins to cancer metastasis. Three exosomal membrane/surface proteins, glucose transporter 1 (GLUT-1), glypican 1 (GPC-1), and disintegrin and metalloproteinase domain-containing protein 10 (ADAM10), were identified as potential breast cancer biomarkers and validated with Western blotting and high-resolution flow cytometry. We demonstrated that exosomes are a rich source of breast cancer-related proteins and surface biomarkers that may be used for disease diagnosis and prognosis.
Value of modified axial review radiograph in diagnosing calcaneal fractures
Authors: Guo Zhe , Yu Bao-Hai , Han Shu-Man , Cao Lei , Wu Hui-Zhao , Zhang Zhu-Zuo , Wu Wen-Juan , Gao Bu-Lang 
Published: August 2020

AbstractTo investigate the value of modified calcaneal axial radiograph—the horizontal calcaneal axial radiograph in diagnosing calcaneal fractures, patients who had acute calcaneal fractures or internal fixation were enrolled, and three groups were established, including the acute fracture group (n = 20), the internal fixation group (n = 20), and the healthy control group (n = 20). All the subjects had regular and modified calcaneal axial radiograph for comparison. In analysis of the results, all volunteers could have ankle dorsiflexion at different degrees. When the ankle was at 30º dorsiflexion for regular axial radiograph, the subtalar joint and the sustentaculum tali could not be clearly displayed. The calcaneus was elongated if the tube tilted in a larger angle but shortened if the tube titled in a smaller angle. When the ankle was at neutral (0º dorsiflexion) location with the tube tilting 45° cephalad or when the ankle was at 20° plantarflexion with the tube tilting 35° cephalad, the subtalar joint, sustentaculum tali, calcaneal body and internal and external calcaneal processes could all be clearly demonstrated. No significant difference (P = 0.79) existed in displaying the bony anatomical structures in regular compared with modified calcaneal axial radiography. For patients with acute calcaneal factures or with internal fixation, the modified calcaneal axial radiography could display more significantly clearly (P = 0.001) bony anatomical structures than the regular one. In conclusion, the modified calcaneal axial radiograph can be performed easily and can clearly show the bony structure of the calcaneus and surrounding bones without adding pain to the patients with calcaneal fractures.
Molecular determinants of SR-B1-dependent Plasmodium sporozoite entry into hepatocytes
Authors: Langlois Anne-Claire , Manzoni Giulia , Vincensini Laetitia , Coppée Romain , Marinach Carine , Guérin Maryse , Huby Thierry , Carrière Véronique , Cosset François-Loïc , Dreux Marlène , Rubinstein Eric , Silvie Olivier 
Published: August 2020

AbstractSporozoite forms of the Plasmodium parasite, the causative agent of malaria, are transmitted by mosquitoes and first infect the liver for an initial round of replication before parasite proliferation in the blood. The molecular mechanisms involved during sporozoite invasion of hepatocytes remain poorly understood. Two receptors of the Hepatitis C virus (HCV), the tetraspanin CD81 and the scavenger receptor class B type 1 (SR-B1), play an important role during the entry of Plasmodium sporozoites into hepatocytes. In contrast to HCV entry, which requires both CD81 and SR-B1 together with additional host factors, CD81 and SR-B1 operate independently during malaria liver infection. Sporozoites from human-infecting P. falciparum and P. vivax rely respectively on CD81 or SR-B1. Rodent-infecting P. berghei can use SR-B1 to infect host cells as an alternative pathway to CD81, providing a tractable model to investigate the role of SR-B1 during Plasmodium liver infection. Here we show that mouse SR-B1 is less functional as compared to human SR-B1 during P. berghei infection. We took advantage of this functional difference to investigate the structural determinants of SR-B1 required for infection. Using a structure-guided strategy and chimeric mouse/human SR-B1 constructs, we could map the functional region of human SR-B1 within apical loops, suggesting that this region of the protein may play a crucial role for interaction of sporozoite ligands with host cells and thus the very first step of Plasmodium infection.
Examining multimorbidity differences across racial groups: a network analysis of electronic medical records
Authors: Kalgotra Pankush , Sharda Ramesh , Croff Julie M. 
Published: August 2020

AbstractHealth disparities across ethnic or racial groups are typically examined through single behavior at a time. The syndemics and multimorbidity health disparities have not been well examined by race. In this study, we study health disparities by identifying the networks of multimorbidities among individuals from seven population groups based on race, including White, African American, Asian, Hispanic, Native American, Bi- or Multi-racial and Pacific Islander. We examined a large electronic medical record (EMR) containing health records of more than 18.7 million patients and created multimorbidity networks considering their lifetime history from medical records in order to compare the network properties among seven population groups. In addition, the networks at organ system level depicting the relationship among disorders belonging to different organ systems are also compared. Our macro analysis at the organ-level indicates that African-Americans have a stronger multimorbidity network followed by Whites and Native Americans. The networks of Asians and Hispanics are sparse. Specifically, the relationship of infectious and parasitic disorders with respiratory, circulatory and genitourinary system disorders is stronger among African Americans than others. On the other hand, the relationship of mental disorders with respiratory, musculoskeletal system and connective tissue disorders is more prevalent in Whites. Similar other disparities are discussed. Recognition and explanation of such differences in multimorbidities inform the public health policies, and can inform clinical decisions as well. Our multimorbidity network analysis identifies specific differences in diagnoses among different population groups, and presents questions for biological, behavioral, clinical, social science, and policy research.
Colony co-founding in ants is an active process by queens
Authors: Aron Serge , Deneubourg Jean-Louis 
Published: August 2020

AbstractCooperative breeding may be selected for in animals when, on average, it confers greater benefits than solitary breeding. In a number of eusocial insects (i.e., ants, bees, wasps, and termites), queens join together to co-create new nests, a phenomenon known as colony co-founding. It has been hypothesised that co-founding evolved because queens obtain several fitness benefits. However, in ants, previous work has suggested that co-founding is a random process that results from high queen density and low nest-site availability. We experimentally examined nest-founding behaviour in the black garden ant,Lasius niger. We gave newly mated queens the choice between two empty nesting chambers, and compared their distribution across the two chambers with that expected under random allocation. We found that queens formed associations of various sizes; in most instances, queens group together in a single chamber. Across all experiments, the frequency of larger groups of queens was significantly higher than expected given random assortment. These results indicate colony co-founding in ants may actually be an active process resulting from mutual attraction among queens. That said, under natural conditions, ecological constraints may limit encounters among newly mated queens.
Longitudinal association between adolescent work values and mental health and well-being in adulthood: a 23-year prospective cohort study
Authors: Fukasawa Maiko , Watanabe Kazuhiro , Nishi Daisuke , Kawakami Norito 
Published: August 2020

AbstractPersonal values developed in adolescence may influence mental health and well-being over the life course. Using data from 684 respondents in the Youth Development Study (61.9% of respondents in the baseline survey), we explored the effects of work values at ages 14–15 on positive and negative emotions, as well as psychological resources (self-esteem and mastery), at ages 37–38. We adjusted for socioeconomic status and the baseline scores of these outcomes using linear regression analyses. Having the work value of getting ahead in adolescence was positively associated with self-esteem in adulthood. Work values in adolescence did not predict positive emotions, negative emotions, or mastery in adulthood. Valuing one’s growth in adolescence may help develop self-esteem in adulthood.
Epigenetics meets GPCR: inhibition of histone H3 methyltransferase (G9a) and histamine H3 receptor for Prader–Willi Syndrome
Authors: Reiner David , Seifert Ludwig , Deck Caroline , Schüle Roland , Jung Manfred , Stark Holger 
Published: August 2020

AbstractThe role of epigenetic regulation is in large parts connected to cancer, but additionally, its therapeutic claim in neurological disorders has emerged. Inhibition of histone H3 lysineN-methyltransferase, especially G9a, has been recently shown to restore candidate genes from silenced parental chromosomes in the imprinting disorder Prader–Willi syndrome (PWS). In addition to this epigenetic approach, pitolisant as G-protein coupled histamine H3receptor (H3R) antagonist has demonstrated promising therapeutic effects for Prader–Willi syndrome. To combine these pioneering principles of drug action, we aimed to identify compounds that combine both activities, guided by the pharmacophore blueprint for both targets. However, pitolisant as selective H3R inverse agonist with FDA and EMA-approval did not show the required inhibition at G9a. Pharmacological characterization of the prominent G9a inhibitor A-366, that is as well an inhibitor of the epigenetic reader protein Spindlin1, revealed its high affinity at H3R while showing subtype selectivity among subsets of the histaminergic and dopaminergic receptor families. This work moves prominent G9a ligands forward as pharmacological tools to prove for a potentially combined, symptomatic and causal, therapy in PWS by bridging the gap between drug development for G-protein coupled receptors and G9a as an epigenetic effector in a multi-targeting approach.
Evidence on the formation of dimers of polycyclic aromatic hydrocarbons in a laminar diffusion flame
Authors: Faccinetto Alessandro , Irimiea Cornelia , Minutolo Patrizia , Commodo Mario , D’Anna Andrea , Nuns Nicolas , Carpentier Yvain , Pirim Claire , Desgroux Pascale , Focsa Cristian , Mercier Xavier 
Published: August 2020

AbstractThe role of polycyclic aromatic hydrocarbons (PAHs) in the formation of nascent soot particles in flames is well established and yet the detailed mechanisms are still not fully understood. Here we provide experimental evidence of the occurrence of dimerization of PAHs in the gas phase before soot formation in a laminar diffusion methane flame, supporting the hypothesis of stabilization of dimers through the formation of covalent bonds. The main findings of this work derive from the comparative chemical analysis of samples extracted from the gas to soot transition region of a laminar diffusion methane flame, and highlight two different groups of hydrocarbons that coexist in the same mass range, but show distinctly different behavior when processed with statistical analysis. In particular, the identified hydrocarbons are small-to-moderate size PAHs (first group) and their homo- and heterodimers stabilized by the formation of covalent bonds (second group).
Optical and theoretical study of strand recognition by nucleic acid probes
Authors: Domljanovic Ivana , Taskova Maria , Miranda Pâmella , Weber Gerald , Astakhova Kira 
Published: August 2020

AbstractDetection of nucleic acids is crucial to the study of their basic properties and consequently to applying this knowledge to the determination of pathologies such as cancer. In this work, our goal is to determine new trends for creating diagnostic tools for cancer driver mutations. Herein, we study a library of natural and modified oligonucleotide duplexes by a combination of optical and theoretical methods. We report a profound effect of additives on the duplexes, including nucleic acids as an active crowder. Unpredictably and inconsistent with DNA+LNA/RNA duplexes, locked nucleic acids contribute poorly to mismatch discrimination in the DNA+LNA/DNA duplexes. We develop a theoretical framework that explains poor mismatch discrimination in KRAS oncogene. We implement our findings in a bead-bait genotyping assay to detect mutated human cancer RNA. The performance of rationally designed probes in this assay is superior to the LNA-primer polymerase chain reaction, and it agrees with sequencing data.
Dynamical symmetry of strongly light-driven electronic system in crystalline solids
Authors: Nagai Kohei , Uchida Kento , Yoshikawa Naotaka , Endo Takahiko , Miyata Yasumitsu , Tanaka Koichiro 
Published: August 2020

AbstractThe Floquet state, which is a periodically and intensely light-driven quantum state in solids, has been attracting attention as a novel state that is coherently controllable on an ultrafast time scale. An important issue has been to demonstrate experimentally novel electronic properties in the Floquet state. One technique is light scattering spectroscopy, which offers an important clue to clarifying the symmetries and energy structures of the states through symmetry analysis of the polarization selection rules. Here, we determine circular and linear polarization selection rules of light scattering in a mid-infrared-driven Floquet system in monolayer MoS2 and provide a comprehensive understanding in terms of the “dynamical symmetry” of the Floquet state.
Exploring the Validity and Operational Impact of Using Allied Health Assistants to Conduct Dysphagia Screening for Low-Risk Patients Within the Acute Hospital Setting
Authors: Schwarz Maria , Ward Elizabeth C. , Cornwell Petrea , Coccetti Anne , D'Netto Pamela , Smith Aimee , Morley-Davies Katharine 
Published: August 2020

Purpose
The purpose of this study was to examine (a) the agreement between allied health assistants (AHAs) and speech-language pathologists (SLPs) when completing dysphagia screening for low-risk referrals and at-risk patients under a delegation model and (b) the operational impact of this delegation model.

Method

All AHAs worked in the adult acute inpatient settings across three hospitals and completed training and competency evaluation prior to conducting independent screening. Screening (pass/fail) was based on results from pre-screening exclusionary questions in combination with a water swallow test and the Eating Assessment Tool. To examine the agreement of AHAs' decision making with SLPs, AHAs (
n
= 7) and SLPs (
n
= 8) conducted an independent, simultaneous dysphagia screening on 51 adult inpatients classified as low-risk/at-risk referrals. To examine operational impact, AHAs independently completed screening on 48 low-risk/at-risk patients, with subsequent clinical swallow evaluation conducted by an SLP with patients who failed screening.

Results

Exact agreement between AHAs and SLPs on overall pass/fail screening criteria for the first 51 patients was 100%. Exact agreement for the two tools was 100% for the Eating Assessment Tool and 96% for the water swallow test. In the operational impact phase (
n
= 48), 58% of patients failed AHA screening, with only 10% false positives on subjective SLP assessment and nil identified false negatives.

Conclusion
AHAs demonstrated the ability to reliably conduct dysphagia screening on a cohort of low-risk patients, with a low rate of false negatives. Data support high level of agreement and positive operational impact of using trained AHAs to perform dysphagia screening in low-risk patients.
Esophageal Fistula following Anterior Cervical Discectomy and Fusion in Traumatic Cervical Injury Cases: A Review
Authors: Meena Rajesh K. , Doddamani Ramesh S. , Sawarkar Dattaraj P. , Singh Pankaj K. , Agarwal Deepak 
Published: August 2020

AbstractBackground Esophageal fistula is a very rare complication associated with anterior approaches to the cervical spine. Timely diagnosis and early intervention are associated with favorable outcomes. There is a dilemma in the literature for its optimal management. In this review article, we will discuss the management dilemmas in patients with esophageal perforation along with an illustrative case.Material and Methods A 24-year-old male patient operated for C5–6 fracture dislocation, presented with esophageal fistula 1 month after surgery. Investigations were done to localize the site of fistula but definitive site of leak could not be identified. Patient was counselled regarding possible surgical intervention; however, patient was opted for conservative treatment.Conclusion Esophageal fistula is a very rare occurrence. Because of varied clinical presentation and frequent delay in diagnosis, a very high index of suspicion should always be kept in mind while evaluating a patient postoperatively for dysphagia. Timely diagnosis and early intervention are key to a successful outcome.
An Unusual Presentation of Growing Skull Fracture with Sutural Diastasis
Authors: Sharma Gaurav , Jain Gaurav , Shekhawat Jitendra , Chopra Sanjeev , Sinha Virendra Deo 
Published: August 2020

AbstractGrowing skull fracture (GSF) is a rare complication of childhood skull fractures, which is caused by progressive diastatic enlargement of the fracture line. Progressive swelling is the most common presenting feature. The most common site is the parietal region. Sutural diastasis is a rare site for development of GSF. Early treatment is must as they cause delayed onset neurological deficit and cranial asymmetry. The aim of this report is to discuss an unusual presentation of GSF with sutural diastasis and review of literature.
Spectrum of Aminoglycoside Modifying Enzymes in Gram-Negative Bacteria Causing Human Infections
Authors: Lalitha Aishwarya Kayanam Vijaya , Venkataramana Geetha Pacha , Eswaran Sudalai , Mariappan Shanthi , Sekar Uma 
Published: August 2020

AbstractIntroduction Aminoglycosides are formidable broad-spectrum antibiotics used in clinical settings; woefully their usage has been reduced by the emergence and distribution of resistance mainly due to aminoglycoside modifying enzymes (AME).Purpose This study was performed to determine the diverse prevalence of AME and their pattern of occurrence in the clinical isolates of gram-negative bacteria. This study also aimed to detect the presence of AMEs that are prevalent in gram-positive bacteria, among gram negatives.Materials and Methods A total number of 386 clinical isolates were included in this study. Polymerase chain reaction revealed the prevalence rate of AMEs screened [aac(6′)-lb, aac(3′)-I, aac(3′)-II, aac(3′)-VI, ant(2′)-I, ant(4′)-IIb, aac(3′)-III, aac(3′)-IV, aph(2′)-Ib, aph(2′)-Ic, aph(2′)-Id, aac (6′)-Ie- aph(2′)-Ia, and aph(3′)-IIIa]. Conjugation experiment was performed for the clinical isolates which harbored any one of the AME which was prevalent in gram-positive bacteria [aph(3′)-IIIa, aac(6′)-Ie-aph(2′)-Ia].Results aac(6′)-lb is the most prevalent AME, followed by aac(3′)-I, aph(3′)-VI, aac(3′)-VI, and aac(3′)-II. The AMEs such as ant (2′)-I, ant(4′)-IIb, aac(3′)-III, aac(3′)-IV, aph(2′)-Ib, aph(2′)-Ic, and aph(2′)-Id were not established in our study isolates. The rate of prevalence of aph(3′)-IIIa, aac(6′)-Ie-aph(2′)-Ia—the AMEs encountered in gram-positive and their co-existence was 19.68% and the conjugation experiment revealed their transfer via plasmids.Conclusion This is the first report from India revealing the presence and prevalence of AMEs which are often encountered among gram-positive bacteria in gram negatives and their presence on conjugative plasmids.
The Yield of Repeat Angiography in Angiography-Negative Spontaneous Subarachnoid Hemorrhage
Authors: Yeole Ujwal , Nagesh Madhusudhan , Shukla Dhaval , R. Aravind H. , R. Prabhuraj A. 
Published: August 2020

Abstract

Objective Despite the technological advancement in imaging, digital subtraction angiography (DSA) remains gold standard imaging modality for spontaneous subarachnoid hemorrhage (SAH). But even after DSA, around 15% of SAH remains elusive for the cause of the bleed. This is an institutional review to solve the mystery, “when is second DSA really indicated?”

Methods In a retrospective review from January 2015 to December 2017, we evaluated cases of spontaneous SAH with initial negative DSA with repeat DSA after 6 weeks to rule out vascular abnormality. The spontaneous SAH was confirmed on noncontrast computed tomography (NCCT) and divided into two groups of perimesencephalic SAH (PM-SAH) or nonperimesencephalic SAH (nPM-SAH). The outcome was assessed by a modified Rankin’s score (mRS) at 6 months postictus.

Results During the study period, we had 119 cases of initial negative DSA and 98 cases (82.3%) underwent repeat DSA after 6 weeks interval. A total of 53 cases (54.1%) had PM-SAH and 45 cases (45.9%) had nPM-SAH. Repeat DSA after 6 weeks showed no vascular abnormality in 53 cases of PM-SAH and in 2 (4.4%) out of 45 cases of nPM-SAH. At 6 months postictus, all cases of PM-SAH and 93% of nPM-SAH had mRS of 0.

Conclusion We recommend, a repeat DSA is definitely not required in PM-SAH, but it should be done for all cases of nPM-SAH, before labeling them as nonaneurysmal SAH. Although the overall outcome for nonaneurysmal spontaneous SAH is better than aneurysmal SAH, nPM-SAH has poorer eventual outcome compared to PM-SAH.