bluuebunny/crossref_metadata_2025_split huggingface dataset:
‘ARE YOU STILL THERE?’ EXPERIENCING SONIC BOTHY'S VERBAAAAATIM
Authors: Docherty Claire , Iddon Martin , Jensenius Alexander Refsum , MacDonald Raymond , Stanley Jane 
Published: December 2024

AbstractSonic Bothy is an inclusive experimental and new-music organisation with an ensemble of musicians with and without disabilities and neurodiversities. This article considers their audiovisual piece Verbaaaaatim (2020–21), its form marked by the context of its development and composition during the COVID-19 pandemic, using a set of interlayered perspectives that mirror the formal layers of the piece. Recorded in a single take, it comprises instrumental sounds, spoken words, written words, static and dynamic graphics and videos of the performers, aligned so that the piece seems consistently to flow onwards, although it is not always clear which element impels its forward motion. The article considers, in particular, Verbaaaaatim's presentation of modes of embodied conviviality between its performers, the ways these find resonance in wider histories of experimental music and the ways in which its elements can be understood in an ecological framework as ‘sound actions’.
A cable-driven elbow exoskeleton with variable stiffness actuator for upper limb rehabilitation
Authors: Yang Lei , Zhang Fuhai , Fu Yili 
Published: December 2024

Abstract
Elbow, with complex physiological structure, plays an important role in upper limb motion which can be assisted with exoskeleton in rehabilitation. However, the stiffness of elbow changes while training which decline the comfort and effect of rehabilitation. Moreover, the rotation axis of elbow is changing which will cause secondary injuries. In this paper, we design an elbow exoskeleton with a variable stiffness actuator and a deviation compensation unit to assist elbow rehabilitation. Firstly, we design a variable stiffness actuator by symmetric actuation principle to adapt the change of elbow stiffness. The parameters of the variable stiffness actuator are optimized by motion simulation. Next, we design a deviation compensation unit to follow the rotation axis deviation outside the horizontal plane. The compensation area is simulated to cover the deviation. Finally, simulation and experiments are carried out to show the performance of our elbow exoskeleton. The workspace can meet the need of daily elbow motion while the variable stiffness actuator can adjust the exoskeleton stiffness as expectation.
Propagation dynamics for an epidemic patch model with variable incubation period
Authors: Xu Zhaoquan , Tan Tianwei , Hsu Cheng-Hsiung 
Published: December 2024

Abstract
We study an epidemic patch model that describes the disease spread in population with variable latency due to the differences in immunologic tolerance between individuals. We focus on whether the disease can spread in space that leads to the emergence of epidemic wave, that is the travelling wave solution with constant speed. We first establish some properties of the linearized wave profile equations, which are helpful in obtaining the priori estimates of travelling waves and wave speeds. Then, applying the truncation method and limiting arguments, we can obtain threshold propagation dynamics of the epidemic model. Our result gives a complete characterization of the existence, nonexistence and minimal wave speed of travelling waves. To the best of our knowledge, this is the first time to characterize the propagation dynamics of epidemic patch model with variable latency, which contributes to the understanding of the transmission phenomenon of disease.
Fruiting traits and seedling regeneration of the relict mangrove plant Nypa fruticans Wurmb in China
Authors: Zhang Mengwen , Zhong Cairong , Feng Erhui , Lv Xiaobo , Fang Zanshan , Cheng Cheng 
Published: December 2024

AbstractNypa fruticans Wurmb is both a relic plant and a true mangrove. In China, wild populations are distributed only on Hainan Island and face significant challenges in regeneration from seedlings. This study explored the underlying causes of recruitment limitation by examining seed morphological traits from three distinct populations (Haikou, Wenchang and Wanning) and analysing seed germination and seedling growth characteristics under varying conditions. The key findings are as follows: fruiting and seed-setting rates for N. fruticans were notably low, standing at only 21 and 40%, respectively. The Wanning population exhibited significantly higher rates compared to the other two populations. Under natural conditions, the germination and seedling emergence rates were also modest, at 36.58 and 22.99%, respectively. The germination and emergence rates of the Wanning population were significantly greater than those of the Haikou and Wenchang populations. Meanwhile, seeds from a single population did not differ in germination rates among three in situ N. fruticans habitats, but seedling emergence rates differed significantly. Optimal conditions for seed germination involved a light intensity of 60%, a salinity of 5‰ and a flooding time of 8 h/day. In natural settings, these three environmental factors fall short of the ideal conditions. The study underscores that light, salinity and flooding are primary factors contributing to the limitations in N. fruticans seedling recruitment. In addition to advocating increased investment in scientific research and technology to address seed source issues, we recommend heightened efforts in habitat restoration, in situ conservation and the optimization of relocation and field return strategies to bolster N. fruticans populations.
Trends of Summer Lake Surface Water Temperature on the Tibetan Plateau and Their Response to Climate Change
Authors: Shi Yi , Huang Anning , Wu Yang , Lazhu  , Wen Lijuan 
Published: December 2024

AbstractThe Tibetan Plateau (TP) is covered by numerous lakes, and lake surface water temperature (LSWT) is an essential indicator of climate change, while few observations hinder our understanding of LSWT variation and its causes over TP. This study aims to simulate the summer LSWT long‐term trends of 81 TP lakes during 1980–2018 and quantify the impacts and contributions of atmospheric variables. Results show that TP lakes warmed with 0.32°C decade−1 on average. Northern TP lakes warmed faster than the southern ones (0.44 vs. 0.16°C decade−1) due to stronger trends of atmospheric variables and higher sensitive of colder lakes to atmospheric changes. 55 (67.9%) lakes of the total lakes studied in current work warmed slower than air due to weakened shortwave radiation (SW↓). Attribution analysis suggests that the air warming and wetting over TP dominate lakes' warming. Regarding synthesis contributions, air warming contributed 79.3%, with increased surface air temperature (SAT) and downward longwave radiation (LW↓) accounting for 41.6% and 37.7%, respectively, and air wetting indicated by increased surface specific humidity (SSH) contributed 39.0%, followed by a positive contribution (16.8%) from declined wind speed (WS). The negative contribution (−35.1%) from weakened SW↓ nearly counterbalances the positive effects of increased LW↓. 55.1% of the total synthesis contribution arises from the cross contribution through interactions among atmospheric variables and is mainly reflected in SAT and SSH, accounting for 26.8% and 24.8%, respectively. The findings enhance understanding of climate change impacts on lake systems and offer insights for lake resource management.
Basic Physics Predicts Stronger High Cloud Radiative Heating With Warming
Authors: Gasparini B. , Mandorli G. , Stubenrauch C. , Voigt A. 
Published: December 2024

AbstractRadiative heating of clouds, particularly those in the upper troposphere, alters temperature gradients in the atmosphere, affecting circulation and precipitation in today's and future climates. However, the response of cloud radiative heating to global warming remains largely unknown. We study changes to high cloud radiative heating in a warmer climate, identify physical mechanisms responsible for these changes, and develop a theory based on well‐understood physics to predict them. Our approach involves a stepwise procedure that builds upon a simple hypothesis of an upward shift in cloud radiative heating at constant temperature and gradually incorporates additional physics. We find that cloud radiative heating intensifies as clouds move upward, suggesting that the role of high clouds in controlling atmospheric circulations increases in a warmer climate.
Typhoon‐Induced Near‐Inertial Waves Around Miyakojima Island in 2015
Authors: Zheng Hua , Zhu Xiao‐Hua , Nakamura Hirohiko , Park Jae‐Hun , Jeon Chanhyung , Zhang Chuanzheng , Zhao Ruixiang , Zhu Ze‐Nan , Nishina Ayako , Min Hong Sik , Kim Dong Guk , Na Hanna , Qiao Yu‐Xiang , Hirose Naoki 
Published: December 2024

AbstractIn 2015, four typhoons traversed the regions surrounding the Ryukyu Island Chain, resulting in different near‐inertial waves (NIWs), the characteristics of which were investigated through in situ observations around Miyakojima Island and numerical simulations covering the East Asian marginal seas. The spatial distribution of typhoon‐induced near‐inertial motions was significantly correlated with the typhoon tracks and background currents. Typhoons Chan‐hom and Goni traversed the observation transect, resulting in different spatial patterns of NIWs owing to their different tracks. Observations on both the western and eastern sides of the Ryukyu Island Chain failed to capture NIWs after typhoon Chan‐hom, because they were positioned to the left of the typhoon track where near‐inertial motions were enhanced only in the upper 50 m. In contrast, NIWs were negligible on the left side and energetic on the right side of typhoon Goni. Despite being hundreds of kilometers from the observation transect, typhoons Soudelor and Dujuan induced NIWs with higher energy levels than those induced by typhoons Chan‐hom and Goni. The energy propagation of NIWs after typhoons Soudelor and Dujuan was significantly influenced by background currents. The western boundary currents, including the Kuroshio and Ryukyu Currents, create negative relative vorticity on their right, which works like a waveguide for the poleward advection of NIWs. However, the Ryukyu Current can be impeded by westward‐propagating cyclonic eddies from the North Pacific, which further disrupts the waveguide.
Body composition in prepubertal children with idiopathic premature adrenarche: implications for cardiometabolic health
Authors: Ben Simon Asaf , Yackobovitch-Gavan Michal , Uretzky Adi , Segev-Becker Anat , Perl Liat , Midlij Eyas , Borger Ophir , Brener Avivit , Lebenthal Yael 
Published: December 2024

Abstract
Background
Premature adrenarche (PA) has been reported as a potential precursor of metabolic disease. We aimed to explore the interaction between body composition and cardiometabolic health of prepubertal children with PA.

Methods
This observational study comprised of 87 children with PA (15 boys, 8.0 ± 1.2 years) and 87 healthy sex- and age-matched controls. Body composition was measured by bioelectrical impedance analysis.

Results
Children with PA had a higher median BMI z-scores (P = 0.001), higher median fat percentage (P = 0.006), and lower muscle-to-fat z-scores (P = 0.050) compared to controls. There were no significant group differences in blood pressure percentiles and lipid profiles. Fat percentage of children with PA was positively correlated and the MFR z-score was negatively correlated with: BMI z-score, systolic blood pressure percentile, and atherogenic dyslipidemia index (P < 0.001 for all). There were significant correlations between parental and offspring MFR z-scores in the control group (father-child: r = 0.528, P = 0.020; mother-child: r = 0.359, P = 0.031), but none in the PA group.

Conclusions
Children with PA exhibited an unfavorable body composition in association with their metabolic health even before the onset of puberty. Furthermore, while healthy children displayed heritable body composition traits, children with PA did not, possibly suggesting a relatively greater role for environmental factors in the PA group.

Impact

Prepubertal children with premature adrenarche have a low muscle-to-fat ratio compared to healthy sex- and age- matched controls.

The body composition of prepubertal children with premature adrenarche is associated with their systolic blood pressure percentile and atherogenic dyslipidemia index.

Children with premature adrenarche and healthy weight exhibited higher adiposity compared to healthy weight controls, and those with overweight/obesity showed higher rates of elevated blood pressure and higher dyslipidemic atherogenic indices compared to overweight/obesity controls.

These findings highlight the importance of early identification, intervention, and lifestyle changes for children with premature adrenarche to help prevent cardiometabolic complications.
Increased Herpes simplex virus 1, Toxoplasma gondii and Cytomegalovirus antibody concentrations in severe mental illness
Authors: Andreou Dimitrios , Steen Nils Eiel , Jørgensen Kjetil Nordbø , Ueland Thor , Wortinger Laura A. , Mørch-Johnsen Lynn , Drabløs Ina , Calkova Tereza , Yolken Robert H. , Andreassen Ole A. , Agartz Ingrid 
Published: December 2024

AbstractInfections with Cytomegalovirus (CMV), Herpes simplex virus 1 (HSV1) and Toxoplasma gondii (TG) have been implicated in severe mental illness. All three pathogens have high seroprevalence in the human population, are neurotropic and establish a persistent infection. We hypothesized that exposed (seropositive) patients with severe mental illness would show higher immunoglobulin G (IgG) concentrations than exposed healthy controls (HC). We included 765 patients with severe mental illness (schizophrenia n = 515, bipolar disorder n = 250) and 541 HC. CMV, HSV1 and TG IgG seropositivity and concentrations were measured with immunoassays (seropositivity: CMV, n = 447 patients vs. 296 HC; HSV1, n = 355 vs. 238; and TG, n = 159 vs. 126). Among seropositive participants, patients had higher HSV1 (p < 0.001) and TG (p = 0.003) IgG concentrations than HC. Stratifying by diagnosis, both schizophrenia (p = 0.001) and bipolar disorder (p = 0.001) had higher HSV1 IgG concentrations, while schizophrenia only had higher TG (p = 0.009) and CMV (p = 0.045) IgG concentrations than HC. In SZ, higher HSV1 IgG concentrations were associated with higher psychotic (p = 0.030) and manic (p = 0.008) symptom scores, but only among CMV- or TG-infected patients which suggests synergistic effects. Among all participants, HSV1 IgG concentrations were inversely associated with interleukin-18 (p < 0.001) and positively associated with high-sensitivity C-reactive protein (p = 0.002) and B cell-activating factor (p = 0.004), possibly indicating T cell exhaustion, enhanced inflammation, and increased B-cell response, respectively. Patients with severe mental illness exhibit a heightened immune system response to HSV1, TG, and CMV infections suggesting immune system dysfunction and/or a more severe infection. For HSV1, higher IgG concentrations were linked to a greater clinical burden.
Women’s perspectives of molecular breast imaging: a qualitative study
Authors: Elliott Helen , Allen A. Joy , Forester Nerys D. , Graziadio Sara , Jones W. S. , Lendrem Beverley Clare , Pearce Mark S. , Powell Timothy , Scott Jason , Bray Alison 
Published: December 2024

Abstract

Background
Mammography has poor sensitivity in dense breast tissue. Retrospective studies suggest that Molecular Breast Imaging (MBI), has superior diagnostic accuracy to mammography in women with very dense breast tissue. Women’s perspectives of MBI are unknown, but are crucial to understanding the feasibility of, and routes to, adoption into practice.

Method
Semi-structured interviews with screened and unscreened women explored acceptability of MBI. Data were analysed thematically.

Results
Four themes were generated from nineteen interviews: (1) presumed negative aspects of MBI are acceptable (2) convenience of access, (3) comfort in familiarity and (4) need for shared decisions relating to risk. Presumed negative aspects of MBI, such as radiation dose and forty-minute scan time, were acceptable provided there are benefits. Some participants were concerned about equitable access, such as parking. Participants expressed comfort in existing and familiar screening processes. Participants acknowledged that informing women of their breast density may result in increased anxiety, but it was still felt to be important to ensure women are fully informed of the risks and harms of screening.

Conclusions
Women consider MBI to be an acceptable breast imaging modality. High-quality information enabling informed decision-making is essential.
In vitro model of retinoblastoma derived tumor and stromal cells for tumor microenvironment (TME) studies
Authors: Alefeld Emily , Haase André , Van Meenen Dario , Budeus Bettina , Dräger Oliver , Miroschnikov Natalia , Ting Saskia , Kanber Deniz , Biewald Eva , Bechrakis Nikolaos , Dünker Nicole , Busch Maike Anna 
Published: December 2024

AbstractRetinoblastoma (RB) is an intraocular tumor arising from retinal cone progenitor cells affecting young children. In the last couple of years, RB treatment evolved towards eye preserving therapies. Therefore, investigating intratumoral differences and the RB tumor microenvironment (TME), regulating tumorigenesis and metastasis, is crucial. How RB cells and their TME are involved in tumor development needs to be elucidated using in vitro models including RB derived stromal cells. In the study presented, we established primary RB derived tumor and stromal cell cultures and compared them by RNAseq analysis to identify their gene expression signatures. RB tumor cells cultivated in serum containing medium were more differentiated compared to RB tumor cells grown in serum-free medium displaying a stem cell like phenotype. In addition, we identified differentially expressed genes for RB tumor and stromal derived cells. Furthermore, we immortalized cells of a RB1 mutated, MYCN amplified and trefoil factor family peptid 1 (TFF1) positive RB tumor and RB derived non-tumor stromal tissue. We characterized both immortalized cell lines using a human oncology proteome array, immunofluorescence staining of different markers and in vitro cell growth analyses. Tumor formation of the immortalized RB tumor cell line was investigated in a chicken chorioallantoic membrane (CAM) model. Our studies revealed that the RB stromal derived cell line comprises tumor associated macrophages (TAMs), glia and cancer associated fibroblasts (CAFs), we were able to successfully separate via magnetic cell separation (MACS). For co-cultivation studies, we established a 3D spheroid model with RB tumor and RB derived stromal cells. In summary, we established an in vitro model system to investigate the interaction of RB tumor cells with their TME. Our findings contribute to a better understanding of the relationship between RB tumor malignancy and its TME and will facilitate the development of effective treatment options for eye preserving therapies.
Hydroxyurea blunts mitochondrial energy metabolism and osteoblast and osteoclast differentiation exacerbating trabecular bone loss in sickle cell mice
Authors: Tripathi Ashish Kumar , Dabeer Sadaf , Song Jun , Vikulina Tatyana , Roser-Page Susanne , Alvarez Jessica A. , Archer David. R. , Weitzmann M. Neale 
Published: December 2024

AbstractSickle cell disease (SCD) is a severe hematological disorder characterized by erythrocyte sickling that causes significant morbidity and mortality. Skeletal complications of SCD include a high incidence of bone loss, especially in vertebrae, leading to fragility fractures that contribute to disease burden. Whether hydroxyurea (HU), a front-line therapy for SCD ameliorates bone disease has not been established. To investigate HU action on SCD-related vertebral defects, we used HU-treated “Townes” mice, an SCD animal model and performed high-resolution micro-computed tomography (µCT) imaging to resolve bone volume and micro-architectural structure of cortical and trabecular bone, the two major compartments contributing to bone mass and strength. Our data revealed that cortical bone was significantly diminished in the vertebrae of skeletally mature (representing adults) and immature (representing children) SCD mice, while only mature mice lost trabecular bone mass. Administration of HU ameliorated cortical bone loss in mature SCD mice, but paradoxically promoted trabecular bone decline in both groups. We further investigated the mechanisms of HU action in wild-type C57BL6/J mice. HU caused dose-dependent trabecular bone loss due to diminished osteoclast and osteoblast function, indicative of a low bone turnover state. Mechanistic investigations in vitro revealed that HU impeded osteoblast-progenitor proliferation and early differentiation, and diminished osteoclastogenic cytokine production, blunting osteoclast formation as well as the activity of mature osteoclasts. HU further, suppressed mitochondrial, but not glycolytic energy metabolism in both differentiating osteoblasts and differentiated osteoclasts. Collectively, these findings reveal that despite ameliorating cortical bone loss, HU inhibits trabecular bone formation and resorption, by suppressing mitochondrial energy metabolism and blunting the differentiation and/or activity of osteoblasts and osteoclasts. Together HU drives a low bone turnover state culminating in trabecular bone loss. Further investigation into HU’s impact on bone in SCD patients is warranted for understanding and managing skeletal complications in this population.
Exosomal miR-320d promotes angiogenesis and colorectal cancer metastasis via targeting GNAI1 to affect the JAK2/STAT3 signaling pathway
Authors: Wu Yawen , Zhang Jie , Li Guanghao , Wang Li , Zhao Yajing , Zheng Baibing , Lin Fanfeng , Xie Li 
Published: December 2024

AbstractColorectal cancer is a common malignant tumor, whose growth and metastasis are influenced by numerous factors. MicroRNAs have garnered increasing attention in recent years due to their involvement in tumor development. Exosomes are involved in intercellular signaling and influence tumor development by promoting tumor cell proliferation and metastasis through activation of angiogenesis and other mechanisms. This study aimed to investigate how the exosomes containing miR-320d from colorectal cancer (CRC) cells promote colorectal cancer metastasis by regulating angiogenesis. CRC-derived exosomes containing miR-320d can be transferred to vascular endothelial cells, facilitating their proliferation, invasion, migration, and angiogenesis. By targeting GNAI1, miR-320d in these exosomes reduces GNAI1 levels in endothelial cells, causing more JAK2/STAT3 activation and VEGFA production. This ultimately enhances the migration and angiogenic capacity of vascular endothelial cells. Moreover, CRC patients with high levels of miR-320d in their blood respond better to treatment with bevacizumab. In vivo experiments further proved the role of miR-320d from CRC exosomes in increasing tumor size, blood vessel formation, and the spread of cancer to the liver. In this study, we have demonstrated that exosomal miR-320d promotes cancer cell metastasis and enhances angiogenesis by downregulating GNAI1 expression and enhancing JAK2/STAT3.