bluuebunny/crossref_metadata_2025_split huggingface dataset:
Macranthoidin B restrains the epithelial-mesenchymal transition through COX-2/PGE2 pathway in endometriosis
Authors: Ding Yi , Yang Xiaoqian , Wei Qinghua , Bi Xuanming , Zhang Yuxin , Ma Yuxia , Yang Meisen , Xu Xiaoyu , Li Cong , Wang Qin , Chen Yi 
Published: December 2024

IntroductionMacranthoidin B is one of the primary and unique triterpenoid saponin metabolites from Lonicera macranthoides Hand. –Mazz, which is used to treat endometriosis (EMS) in traditional Chinese medicine. However, the effect of macranthoidin B remains unknown in EMS. This study aimed to elucidate the effect and mechanism of macranthoidin B in EMS.MethodsUsing rat autograft EMS model, the volume of ectopic endothelium, the histopathology, serum E2 and PROG were evaluated after macranthoidin B’s treatment. In primary endometriotic stromal and HEC1-B cells, the invasion and metastasis were assessed by scratch wound and Transwell tests. The epithelial-mesenchymal transition and COX-2/PGE2 pathway were examined in vivo and in vitro. Macranthoidin B were combined with LPS or celecoxib.ResultsIn a rat autograft EMS model, macranthoidin B suppressed ectopic lesion volume, improved histopathological morphology, and regulated serum estradiol (E2) and progesterone (PROG) levels. Additionally, macranthoidin B inhibited invasion and metastasis of primary endometriotic stromal cells and HEC1-B cells. Mechanistically, macranthoidin B suppressed COX-2/PGE2 pathway and epithelial-mesenchymal transition both in vivo and in vitro. LPS, the COX-2/PGE2 pathway activator, showed the promotion of epithelial-mesenchymal transition, invasion and metastasis. Macranthoidin B exhibited the antagonistic effects against LPS. Celecoxib, the COX-2/PGE2 pathway inhibitor, restrained the epithelial-mesenchymal transition, invasion and metastasis. This effect of celecoxib was enhanced by macranthoidin B.Discussion Macranthoidin B prevents epithelial-mesenchymal transition through COX-2/PGE2 pathway in EMS. It will facilitate the macranthoidin B’s development and broaden its potential application.
Optimized automated radiosynthesis of 18F-JNJ64413739 for purinergic ion channel receptor 7 (P2X7R) imaging in osteoporotic model rats
Authors: Lu Yingtong , Cui Yan , Hou Lu , Jiang Yuanfang , Shang Jingjie , Wang Lu , Xu Hao , Ye Weijian , Qiu Yang , Guo Bin 
Published: December 2024

ObjectiveTo optimize the automated radiosynthesis of the purinergic ion channel receptor 7 (P2X7R) imaging agent 18F-JNJ64413739 and evaluate its potential for brain imaging in osteoporotic model rats.MethodsA more electron-deficient nitropyridine was employed as the labeling precursor to facilitate the 18F-labeling. The radiosynthesis was conducted on an AllinOne synthesis module, and followed by purification via high-performance liquid chromatography (HPLC). The resulting 18F-JNJ64413739 was subjected to quality control tests. Small-animal PET/CT imaging studies were performed in sham and osteoporotic model rats.ResultsThe optimized automated radiossynthesis of 18F-JNJ64413739 was successfully completed in approximately 100 min with non-decay-corrected radiochemical yield of 6.7% ± 3.8% (n = 3), &gt;97% radiochemical purity and &gt;14.3 ± 1.3 GBq/μmol molar activity. The product met all clinical quality requirements. 18F-JNJ64413739 PET/CT imaging showed revealed significantly higher radioactivity uptake in various brain regions of the osteoporotic model rats compared to sham control group.ConclusionWe successfully optimized the automated radiosynthesis of 18F-JNJ64413739. The resulting tracer not only met clinical quality requirements but also demonstrated potential for clinical application in the diagnosis of osteoporosis, as evidenced by higher radioactivity uptake in various brain regions of osteoporotic model rats compared to normal controls.
Depression and health literacy among adolescents and adults in Germany: findings from two representative samples
Authors: König Lars , Schröder Rebekka , Hamer Tim , Suhr Ralf 
Published: December 2024

IntroductionDepressive disorders constitute a significant public health challenge. Health literacy might be an important factor to consider in prevention strategies for depressive disorders, which is why this study aimed at exploring the association between depressive symptom levels and severity and health literacy, along with additional sociodemographic factors.Materials and methodsData were collected from two large samples of adults (N = 3,011) and adolescents (N = 1,021) representative of the German-speaking population in Germany. Levels of health literacy (HLS-EU-Q16 questionnaire), depressive symptom severity, rates of depression levels (PHQ-9 questionnaire) and sociodemographic information (age, gender, social status, level of education) were obtained. The associations between sociodemographic factors, health literacy and depression were analyzed using t-tests, analyses of variance and χ2-tests.ResultsOverall, rates of depression were high in both samples (16.5% in adults and 18.4% in adolescents) when measured with the sum score ≥ 10 cut-off criterion and substantially lower when assessed with the diagnostic algorithm criterion (7.2% in adults and 9.8% in adolescents). Rates of depression and severity of depressive symptoms were higher in female than male individuals in both samples. Depressive symptom severity and depression rates increased with increasing age in adolescents and decreased with increasing age in adults. Higher levels of education and lower social status were associated with higher depressive symptom severity and rates in adults, with a more heterogeneous picture in adolescents. In both samples, depressive symptom severity and rates were higher in individuals with poorer health literacy.DiscussionThe results point to a potential role for health literacy in preventing depressive disorders. More research is needed with longitudinal and experimental study designs into the question whether public health interventions targeting health literacy improvements could play a critical role in reducing the burden of depression across different age cohorts.
Developing a codebook for assessing auditory hallucination complexity using mixed methods
Authors: Pietkiewicz Igor J. , Tomalski Radosław , Hełka Anna M. 
Published: December 2024

IntroductionIn recent years there has been a notable expansion of psychotherapeutic approaches to treat people experiencing auditory verbal hallucinations (AVH). While many psychotherapists conceptualize voices as “dissociative parts” and apply therapeutic techniques derived from the field of dissociation, research investigating AVH from this perspective is limited. Despite the acknowledgment that voices encountered in dissociative identity disorder (DID) often exhibit high complexity and autonomy, there is a critical need for assessment tools capable of exploring voice complexity across different clinical groups. Such tools hold significant potential for aiding clinicians to identify patients who may benefit more from dissociation-based therapy approaches. This study aims to operationalize the concept of voice complexity (VC) by identifying its different dimensions and indicators. MethodsUsing concept mapping procedures, 12 healthcare professionals and two voice-hearers participated in brainstorming, and 24 people with clinical backgrounds performed sorting and rating tasks. ResultsSeven dimensions of VC were identified: System Complexity, Content Complexity, Voice’s Interest Complexity, Interaction Complexity with Voice-Hearer, Voice’s Own Life, Voice Influence, and Voice’s Vocal Characteristics. A codebook for assessing VC with indicators for varying levels of complexity across these dimensions was developed and can be used with the Structured Clinical Interview for Voice-Hearers. Inter-rater reliability, measured by comparing the assessments of two interview transcripts by seven raters using Kendall’s Coefficient, indicated substantial agreement in one interview (W = .613) and almost perfect agreement in the second (W = .805). DiscussionThe new instrument has promise as an effective tool for comparative studies exploring VC in diverse clinical and non-clinical populations, with potential implications for clinical practice and future research.
HealthPass: a contactless check-in and adaptive access control system for lowering cluster infection risk in public health crisis
Authors: Luo Guofeng , Wang Yufei , Hong Linghong , He Xin , Wang Jiaru , Shen Qu , Wang Cheng , Chen Longbiao 
Published: December 2024

IntroductionEnsuring effective measures against the spread of the virus is paramount for educational institutions and workplaces as they resume operations amidst the ongoing public health crisis. A touchless and privacy-conscious check-in procedure for visitor assessment is critical to safeguarding venues against potential virus transmission.MethodsIn our study, we developed an interaction-free entry system featuring anonymous visitors who voluntarily provide data. This system introduces an adaptable venue entry management mechanism that accounts for both visitors' potential risk and the venue's capacity, aiming to curb the risk of localized infections. We assess visitors' liability based on their voluntarily provided data through radar map analysis. Additionally, we evaluate the venue's situation by quantifying its risk from multiple dimensions. A queuing model is then employed to control visitor access adaptively based on visitors' liability and the venue's availability.ResultsSince May, our university campus has been the operational site for the implemented system, catering to the needs of visitors across distinct venues. Using real-world implementation, we conduct a series of simulation experiments and case studies to verify the effectiveness of the HealthPass system in lowering infection risks.DiscussionThe system has demonstrated its capacity to reduce infection risks by adapting visitor entry procedures based on individual risk factors and venue conditions. Our results suggest that the integration of a dynamic queuing model and real-time data analysis can effectively manage the flow of visitors while ensuring public health safety.
An educational initiative aimed at increasing antimicrobial resistance awareness among school-going Jordanian youth
Authors: Qenab Basmah , Aqel Tahreer , Younis Hanin , AbuShweimeh Rahmeh , Al Zghoul Anwaar , Sweedan Zaina , Omran Mohammad , Joshi Mohan P. , Konduri Niranjan 
Published: December 2024

BackgroundThe World Health Organization (WHO) has recommended strategies and actions to enhance awareness and understanding of AMR. Gaps in AMR awareness remain in Jordan, particularly among the youth.AimTo describe our programmatic approach to AMR education across Jordanian governorates among school-aged children.MethodsOur approach depicts the development of comprehensive health messages, pilot and expansion phases in schools, and pre- and post-session knowledge assessments.Results2,700 students across 30 schools reached with AMR health messages. Gained knowledge was assessed in 932 students, revealing significant improvements in understanding the importance of consulting a doctor before taking antibiotics, the relationship between nutrition and immunity, the dangers of sharing medicines, and proper handwashing techniques. The average expenditure was approximately $8.55 per student.ConclusionThe intervention underscores the critical role of targeted educational initiatives in improving AMR awareness among youth, emphasizing the need for sustained and scalable approaches to combat AMR effectively.
The impact of track and field training on dynapenia: gender differences in age-related decline of vertical jump performance among older adults
Authors: Fernández-Peña Eneko , Formiglio Eugenio , Gervasi Marco , Benelli Piero , Bertuccioli Alexander , Russo Giuseppe , Giustino Valerio , Patti Antonino 
Published: December 2024

IntroductionAlongside sarcopenia, the age-related loss of muscle strength and power, known as dynapenia, increases the risk of functional disability and mortality in older adults. However, engaging in sporting activities during old age appears to enhance functional capacity. The differences in effects between athletes and sedentary individuals, as well as between genders, have yet to be fully clarified.MethodsThe vertical jump test is recognized as a measure of lower limb performance with almost no learning effect. In the present study, we quantified age-related countermovement jump (CMJ) height loss in 120 subjects over 58 years old among both master athletes and sedentary counterparts, and analysed gender differences.ResultsBoth male and female master athletes showed significantly higher jump heights results than their sedentary counterparts (male athletes 28.5 ± 4.3 cm vs. male sedentaries 15.1 ± 5.2 cm; p &lt; 0.01; female athletes 22.7 ± 2.5 cm vs. female sedentaries 8.2 ± 3.3 cm; p &lt; 0.01). Female athletes were found to have higher CMJ performance than even sedentary men (p &lt; 0.01). The rate of decline in jumping ability was the same for male athletes and non-athletes, but female athletes had the shallower rate of decline of all the groups observed (2.78 cm per decade).DiscussionSporting activity in the older age allows both men and women to perform at a higher level, with the latter also benefiting from a slower rate of decline, which can have a positive impact on functional ability and quality of life.
Unleashing nature's defense: potent antimicrobial power of plant extracts against oral pathogens and Streptococcus mutans biofilms
Authors: Hickl Joachim , Argyropoulou Aikaterini , Al-Ahmad Ali , Hellwig Elmar , Skaltsounis Alexios Leandros , Wittmer Annette , Vach Kirstin , Karygianni Lamprini 
Published: December 2024

ObjectivesThe increasing demand for alternatives to antibiotics against resistant bacteria has led to research on natural products. The aim of this study was to analyze the antimicrobial and antibiofilm activity of 16 Mediterranean herb extracts.Materials and methodsThe extracts were analyzed using High Performance Thin Layer Chromatography. The minimum inhibitory concentration and minimum bactericidal concentration of the extracts from Achillea taygetea, Cistus creticus ssp. creticus, Cistus monspeliensis, Lavandula stoechas, Mentha aquatica, Mentha longifolia, Origanum vulgare, Phlomis cretica, Rosmarinus officinalis, Salvia sclarea, Satureja parnassica, Satureja thymbra, Sideritis euboea, Sideritis syriaca, Stachys spinosa, and Thymus longicaulis were determined against eight oral bacteria and fungus Candida albicans. Microtiter plate test was conducted to evaluate the antibiofilm activity against Streptococcus mutans.ResultsOverall, all tested extracts efficiently suppressed the growth of obligate anaerobic bacteria. When applied at concentrations ≥0.15 mg/ml, the extracts exhibited moderate to high antibiofilm activity comparable to that of chlorhexidine (CHX) against S. mutans. Interestingly, R. officinalis (MIC: 0.01–0.06 mg/ml) and O. vulgare (MIC: 0.04–1.25 mg/ml) demonstrated the highest antibacterial activity against oral bacteria. Additionally, R. officinalis and L. stoechas significantly inhibited S. mutans biofilm formation at 0.15 mg/ml.ConclusionsThe tested plant extracts can be considered as alternative natural antimicrobial and antibiofilm agents.Clinical relevanceMediterranean herb extracts show promise as natural alternatives to combat oral bacteria and biofilm formation, offering potential new therapies for infectious oral diseases in the context of antibiotic resistance.
Mitochondrial dysfunction signatures in idiopathic primary male infertility: a validated proteomics-based diagnostic approach
Authors: Sawaid Kaiyal Raneen , Mukherjee Sromona D. , Panner Selvam Manesh Kumar , Miller Aaron W. , Vij Sarah C. , Lundy Scott D. 
Published: December 2024

Research questionMale infertility accounts for almost half of all infertility cases worldwide, with idiopathic male infertility accounting for up to 30% of the cases. Sperm proteomics has revealed critical molecular pathway changes in men with infertility. However, the sperm mitochondrial proteome remains poorly understood. We attempted to answer the following question: Do patients with idiopathic primary male infertility exhibit a proteomic signature associated with mitochondrial dysfunction that could be used as a target for future mechanistic investigations?DesignPatients with idiopathic primary infertility (20–40 years old) referred to the Cleveland Clinic between March 2012 and April 2014 were compared with fertile donor controls. Sperm proteins were analyzed using sodium dodecyl sulphate-polyacrylamide gel electrophoresis page (SDS-PAGE) and liquid chromatography-mass spectrometry (LC-MS), and differentially expressed proteins (DEPs) were identified based on significance test results and fold change thresholds. Protein expression was validated using western blotting.ResultsProteomic analysis of pooled samples from fertile donors (n = 5) and patients with idiopathic primary infertility (n = 5) identified 1,134 proteins, including 344 DEPs. Mitochondrial dysfunction topped the ingenuity toxicity list. Analysis of expression levels of three mitochondrial proteins known to combat oxidative stress revealed that peroxiredoxin-5 (PRDX5) and superoxide dismutase 2 (SOD2), but not glutathione disulphide reductase, were significantly decreased in patient samples compared with those in fertile-donor samples.ConclusionsThis study revealed an association of downregulated expression of PRDX5 and SOD2 in sperm samples of patients with idiopathic primary male infertility. Our results support future mechanistic studies and development of advanced diagnostic methods to better identify men with mitochondria-related male infertility.
Прогрессирующая мультифокальная лейкоэнцефалопатия в практике врача-невролога: современные представления, клинический случай
Authors: Лурье Т.В. , Дрик О.Н. , Воронцова Ж.В. 
Published: December 2024

Прогрессирующая мультифокальная лейкоэнцефалопатия – прогрессирующее фатальное демиелинизирующее заболевание центральной нервной системы, вызванное реактивацией существующего в латентном состоянии JC-вируса на фоне выраженного иммунодефицита. В то же время в ряде случаев развитие заболевания происходит и при отсутствии тяжелого иммунодефицита. Диагноз прогрессирующей мультифокальной лейкоэнцефалопатии основывается на определении JC-вирусной ДНК методом полимеразной цепной реакции в цереброспинальной жидкости (чувствительность 64–90%, специфичность 100%) и данных магнитно-резонансной томографии головного мозга. Стандартом диагностики считается гистологическое исследование биоптата головного мозга. Специфическое лечение прогрессирующей мультифокальной лейкоэнцефалопатии не разработано. При развитии данной патологии целесообразно отменить или максимально снизить дозу глюкокортикоидов и цитотоксических препаратов. В большинстве случаев заболевание приводит к летальному исходу в течение нескольких месяцев. Представлен клинический случай иммунокомпетентного пациента с установленным диагнозом прогрессирующей мультифокальной лейкоэнцефалопатии. Данное клиническое наблюдение подтверждает сложность диагностики данного заболевания в связи с отсутствием выраженности симптоматики, которая неспецифична, а также с необходимостью подтверждения диагноза дополнительными методами (инструментальными, лабораторным), не всегда доступными в повседневной практике.
Progressive multifocal leukoencephalopathy is a progressive fatal demyelinating disease of the central nervous system caused by reactivation of the latent JC-virus against the background of severe immunodeficiency. However, in some cases, the disease may also occur with no severe immunodeficiency. The diagnosis of progressive multifocal leukoencephalopathy is based on the determination of JC-viral DNA by polymerase chain reaction in cerebrospinal fluid (sensitivity 64–90%, specificity 100%) and data from magnetic resonance imaging of the brain. A histological examination of brain biopsy specimen is considered the diagnostic standard. No specific treatment for progressive multifocal leukoencephalopathy has been established. In this pathology, it is advisable to withdraw or minimize glucocorticoids and cytotoxic medicinal products dosage. In most cases, the disease is fatal within a few months. A case report of an immunocompetent patient with diagnosed progressive multifocal leukoencephalopathy is presented. This clinical observation confirms the diagnostic challenge for this disease due to the lack of symptom severity which are nonspecific, as well as the need for additional methods (instrumental and laboratory) to confirm the diagnosis, which are not always available in routine practice.
Гипофизит с проявлениями гипопитуитаризма у пациента с ВИЧ-инфекцией и раком предстательной железы: клинический случай
Authors: Мохорт Т.В. , Лихорад Н.М. , Даниленко Д.А. , Мохорт Е.Г. , Дурдыгылыджова Д. 
Published: December 2024

Гипофизит включает в себя широкий спектр воспалительных заболеваний, которые приводят к увеличению размеров гипофиза и синдрому компрессии. Это состояние является редкой причиной гипопитуитаризма, патогенез которого до сих пор плохо изучен. Распространенность гипофизита составляет 1 случай на 1 миллион человек. Распознавание новых причин, более широкое использование методов визуализации, а также увеличение количества патологических образцов после операции на гипофизе привели к увеличению заболеваемости гипофизитом. Полифакторность развития гипофизита приобретает особую актуальность в связи с лекарственными, вирусными и соматическими влияниями. Представленное клиническое наблюдение пациента с гипофизитом, развившимся после спленэктомии на фоне ВИЧ-инфекции и метастазирующего рака предстательной железы, оставляет широкое поле для дискуссии по выделению основного этиологического агента. Гипопитуитаризм на фоне гипофизита является состоянием, требующим адекватной заместительной терапии, так как наличие эндокринных дисфункций, особенно надпочечниковой недостаточности, оказывает негативное влияние на качество жизни пациента и определяет высокие риски в стрессовых ситуациях.
Hypophysitis includes a wide range of inflammatory diseases leading to increased pituitary gland size and compression syndrome. This condition is a rare cause of hypopituitarism, the pathogenesis of which is still poorly understood. The incidence of hypophysitis is 1 case per 1 million people. Recognizing new causes, more extensive use of imaging techniques, and increased number of pathological specimens after pituitary surgery have led to an increase in hypophysitis diagnoses. The multifactorial nature of hypophysitis becomes of particular relevance due to medicinal, viral, and somatic impacts. The presented clinical case of a patient with hypophysitis occurring after splenectomy against HIV infection and metastatic prostate cancer leaves a wide field for discussion aimed at identifying the main etiologic agent. Hypopituitarism on the background of hypophysitis is a condition requiring adequate replacement therapy, since endocrine dysfunctions, especially adrenal insufficiency, impact negatively patients’ quality of life and determine high risks in stressful situations.