bluuebunny/crossref_metadata_2025_split huggingface dataset:
Molecular detection of Staphylococcal enterotoxins and mecA genes products in selected food samples collected from different areas in Khartoum state.
Authors: Yahya Mohammed , Ali Hashim Abdalbagi , Gorish Babbiker Mohammed Taher , Ali Sara Omer , Abdalrhim Eman Saif Aldein , Mergani Mawada Hamza , Elgadir Asmaa Abass Abd , Mohammed Somaya Khalid , Ahmed Salma Omer , Musa Naglaa Alsaeid , Ahmed Alaa Saeed , Abdalla Wafaa Mohammed , Hamedelnil Yousif Fadlallah , Hashim Ahmed Ibrahim , Altayeb Hisham N. 
Published: December 2020

Abstract
Background: Staphylococcal food poisoning is an intoxication that results from the consumption of improperly prepared or stored foods containing sufficient amounts of one or more preformed S. aureus enterotoxins. Nowadays many researchers worldwide noted an emergence of resistant strains Staphylococci particularly for the antibiotic methicillin. Therefore, this study was aimed to determine the existence of Staphylococcus aureus and its enterotoxins, mecA genes in selected food samples.Results: A total of 400 selected food samples were collected from different areas in Khartoum state. The selected foods included cheese, meat products, fish and raw milk. One hundred sample from each type of food were cultivated and the resultant growth yielded 137 (34.25 %) S. aureus, 126 (31.5%) bacteria other than S. aureus and 137 (34.25%) yielded no growth. Eighty-four of the137 S. aureus isolates were randomly selected and tested for the presence of mecA and enterotoxin genes. Oxacillin sensitivity test showed that 15(11%) of 137 S. aureus isolates were Oxacillin resistant. The PCR assay showed that the mecA gene was detected in 15 of 84 (17%) S. aureus isolates. Simultaneously, only 2 (2.385%) out of 84 S. aureus isolates showed an enterotoxin B gene product. Conclusion: There was a relatively moderate prevalence of methicillin-resistant staphylococcus aureus with very low frequency of enterotoxin B gene in different kinds of selected food samples that collected from Khartoum state. These findings elucidate the increased risk on public in Khartoum being affected by Staphylococcal food poisoning upon consumption of dairy or meat products prepared in unhygienic conditions that could lead to intoxication by Staphylococcus aureus enterotoxins.
Extended Adverse Effects of Cyclophosphamide on Mouse Ovarian Function
Authors: Kim Jihyun , You Sooseong 
Published: December 2020

Abstract
Background: Most patients with cancer undergo multiple administrations of anticancer drugs during treatment, resulting in chronic impairment of their reproductive health. As improved treatment options increase cancer survival, it has become increasingly important to address fertility issues in cancer survivors. In this study, we examined the pathophysiological effects of multiple exposures to cyclophosphamide (Cy) on the ovaries of mice and their underlying molecular mechanism. Methods: Female C57BL/6 mice were intraperitoneally injected with 100 mg/kg Cy six times over 2 weeks; 4 weeks later, the mice were sacrificed and their ovaries, sera, and oocytes were collected for histological observation, measurement of anti-Müllerian hormone levels, and assessment of oocyte quantity and quality in response to hormonal stimulation. Gene expression changes in Cy-treated ovaries were examined by microarray and bioinformatics analyses. Results: After repeated Cy exposure, the anti-Müllerian hormone level was decreased, and follicle loss and impairments in the quality of oocyte were irreversible. The expression levels of genes involved in folliculogenesis, oogenesis, and zona pellucida glycoprotein transcription displayed sustained alterations in Cy-exposed ovaries even after 4 weeks.Conclusion: The adverse effects of Cy on ovarian function and oocytes remained even after chemotherapy was complete. Therefore, strategies to prevent ovarian damage or restore ovarian function after treatment are required to safeguard the fertility of young cancer survivors.
Expression of Substance P, Calcitonin Gene-Related Peptide and Vascular Endothelial Growth Factor in human dental pulp under different clinical stimuli
Authors: Caviedes-Bucheli Javier , Lopez-Moncayo Luis Fernando , Munoz-Alvear Hernan Dario , Gomez-Sosa Jose Francisco , Diaz-Barrera Luis Eduardo , Curtidor Hernando , Munoz Hugo Roberto 
Published: December 2020

Abstract
Background To quantify the expression of SP, CGRP, and VEGF in human dental pulps as a response to occlusal trauma, moderate orthodontic forces, and occlusal trauma plus moderate orthodontic forces simultaneously.Methods Forty human dental pulp samples were collected from healthy premolars indicated for extraction for orthodontic reasons in 20 patients. Patients were divided into four groups with 10 samples each (5 lower and 5 upper premolars from each patient): Healthy pulp control group, occlusal trauma group, moderate orthodontic forces group; and occlusal trauma plus moderate orthodontic forces group. Stimuli were applied for 24 h before tooth extraction in all experimental groups. All samples were processed, and SP, CGRP, and VEGF were measured by radioimmunoassay. The ANOVA test was performed to establish significant differences between groups and Tukey’s HSD post hoc comparisons were also performed.Results The highest increase in SP, CGRP, and VEGF expressions was found in the occlusal trauma plus orthodontic forces group, followed by the moderate orthodontic forces, the occlusal trauma and the control groups, with statistically significant differences between all groups (ANOVA p<0.001). All pairwise post-hoc comparisons were also significant (Tukey HSD p<0.001).Conclusion SP, CGRP, and VEGF expressions significantly increase in human dental pulps when stimulated by occlusal trauma combined with moderate orthodontic forces, as compared with these two stimuli applied independently. Name of the registry: Importance of Neurogenic Inflammation in the Angiogenic Response of the Dental Pulp as a Defensive ResponseTrial registration number: NCT03804034Date of registration: 01/15/2019 Retrospectively registeredURL of trial registry record: https://clinicaltrials.gov/ct2/show/NCT03804034?term=NCT03804034&draw=2&rank=1
The evaluation of maximum condyle-tragus distance can predict difficult airway management without exposing upper respiratory tract: a prospective observational study
Authors: Wu Hao , Hu Dandan , Chen Xu , Zhang Xuebing , Xia Min , Chai Xiaoqing , Wang Sheng , Zhang Wei 
Published: December 2020

Abstract
Background: Routine preoperative methods to assess airway such as the interincisor distance(IID), Mallampati classification, and the upper lip bite test(ULBT) have a certain risk of upper respiratory tract exposure and virus spread. The condyle-tragus maximal distance(C-TMD) can be used to assess the airway, and the assessment method does not require the patient to expose the upper respiratory tract, but its value in predicting difficult laryngoscopy compared to other indicators (Mallampati classification, IID, and ULBT) remains unknown. The purpose of this study was to observe the value of C-TMD to predict difficult laryngoscopy and the influence on intubation time and intubation attempts, and provide a new idea for preoperative airway assessment during epidemic. Methods: We enrolled adult patients who underwent general anesthesia and tracheal intubation. The IID, Mallampati classification, ULBT, and the C-TMD of each patient were evaluated prior to the initiation of anesthesia. The primary outcome was intubation time. The second outcome were difficult laryngoscopy defined as the Cormack-Lehane Level > grade 2 and the number of intubation attempts. Results: A total of 304 patients were successfully included in the study, 39 patients were identified as difficult laryngoscopy. The intubation time was shorter with the C-TMD＞1 finger group 46.8±7.3s, compared with the C-TMD＜1 finger group 50.8±8.6s (p＜0.01). First attempt success rate was higher in the C-TMD＞1 finger group 98.9% than in the C-TMD＜1 finger group 87.1% (P＜0.01). The correlation between the C-TMD and Cormack-Lehane Level was 0.317(Spearman correlation coefficient, P＜0.001), and the area under the ROC curve was 0.699(P＜0.01). The C-TMD <1 finger width was the most consistent with difficult laryngoscopy (κ=0.485;95%CI:0.286-0.612) and its OR value was 10.09(95%CI: 4.19-24.28), sensitivity was 0.469(95%CI: 0.325-0.617), specificity was 0.929(95%CI: 0.877-0.964), positive predictive value was 0.676 (95%CI: 0.484-0.745), negative predictive value was 0.847(95%CI :0.825-0.865).Conclusion: Compared with the IID , Mallampati classification and ULBT, the C-TMD has higher value in predicting difficult laryngoscopy and does not require the exposure of upper respiratory tract.Trial registration: The study was registered on October 21, 2019 in the Chinese Clinical Trial Registry (ChiCTR1900026775).
Individual Approach for Treatment of Primary Intestinal Lymphangiectasia in Children: Single-center Experience and Review of the Literature
Authors: Kwon Yiyoung , Kim Eun Sil , Choe Yon Ho , KIM Mi Jin 
Published: December 2020

Abstract
Background: Intestinal lymphangiectasia is a rare disease. Thus, prospective studies are impossible, and therapy is still controversial. Several medicines are suggested for treatment but there are no existing indications for drug choice and treatment guidelines. We aimed to introduce the action mechanism of each drug and treatment overview in a single-center experience and a review of the literature on second-line therapy for primary intestinal lymphangiectasia.Method: Children under 18 years old diagnosed with intestinal lymphangiectasia from June 2000 to June 2020 were included and retrospectively reviewed in the study. Capsule endoscopy, MR lymphangiography, or whole-body MRI for investigating the extent of abnormal lymphatic vessels in addition to endoscopy and biopsy were conducted. The individual treatment approaches depended upon the lymphangiectasis locations involved.Results: Only one patient showed a response to dietary therapy. One patient was successfully cured after two therapeutic lymphatic embolization. Octreotide was tried for two patients who had extensive lymphangiectasis. Lymphangiectasis recurred when octreotide was used for three months in one patient, and there was no effect in the other patient. Sirolimus was tried for four patients. Two of them had abnormal lymphatic lesions only in the intestine, and the others had extensive lymphangiectasis. The former group showed clinical improvement after 3 – 4 months of sirolimus treatment, whereas the latter group showed clinical improvement only after one month of sirolimus treatment.Conclusion: Surgery or embolization is a potential therapeutic option for patients with focal abnormal lymphatic lesions. Octreotide is not an optimal choice for patients with extensive lymphangiectasis. Sirolimus is an effective and safe drug and can be the first drug of choice for patients with extensive lymphangiectasis.
The Role of Intellectual Property Rights on Access to Medicines in the WHO African Region: 25 Years After the TRIPS Agreement
Authors: Motari Marion , Nikiema Jean-Baptiste , Kasilo Ossy Muganga Julius , Kniazkov Stanislav , Loua Andre , Sougou Aissatou , Tumusiime Prosper 
Published: December 2020

Abstract
Background It is now 25 years since the adoption of the Agreement on Trade-Related Aspects of Intellectual Property Rights (TRIPS) and the same concerns raised during its negotiations such as high prices of medicines, market exclusivity and delayed market entry for generics remain relevant as highlighted recently by the Ebola and COVID-19 pandemics. The World Health Organization’s (WHO) mandate to work on the interface between intellectual property, innovation and access to medicine has been continually reinforced and extended to include providing support to countries on the implementation of TRIPS flexibilities in collaboration with stakeholders. This study analyses the role of intellectual property on access to medicines in the African Region. Methods We analyze patent data from the African Regional Intellectual Property Organization (ARIPO) and Organisation Africaine de la Propriété Intellectuelle (OAPI) to provide a situational analysis of patenting activity and trends. We also review legislation to assess how TRIPS flexibilities are implemented in countries.Results Patenting was low for African countries. Only South Africa and Cameroon appeared in the list of top ten originator countries for ARIPO and OAPI respectively. Main diseases covered by African patents were HIV/AIDS, cardiovascular diseases, cancers and tumors. Majority countries have legislation allowing for compulsory licensing and parallel importation of medicines, while the least legislated flexibilities were explicit exemption of pharmaceutical products from patentable subject matter, new or second use of patented pharmaceutical products, imposition of limits to patent term extension and test data protection. 39 countries have applied TRIPS flexibilities, with the most common being compulsory licensing and LDC transition provisions. Discussion Countries of the WHO African Region operate within a multi-layered IP regulatory landscape globally and at regional level which is characterized by inconsistencies and misalignment which limit the extent to which countries can implement TRIPS flexibilities. Conclusions Opportunities exist for WHO to work with ARIPO and OAPI to support countries in reviewing their legislation to be more responsive to public health needs.
Factors Affecting Women Entrepreneurs’ Success; A study of Small and Medium-Sized Enterprises in Emerging Market of Pakistan
Authors: KHAN RIZWAN Ullah , Salamzadeh Yashar , Shah Syed Zulfiqar Ali , Hussain Mazhar 
Published: December 2020

Abstract
In the present era, women are recognized as successful entrepreneurs through their strong desire, qualities and capabilities for robust economic development. Due to such an important contribution of women in economic development, we propose to investigate the factors, which affect women entrepreneur’s success in Pakistan. Data collected through structured questionnaires from 181 registered SMEs operating in Pakistan. A conceptual model is developed, while SPSS and AMOS software’s are used for analysis. The results indicate that the internal factors; including the need for achievements, risk-taking and self-confidence and external factors including economic factors and socio-cultural factors have a positive and significant influence on the success of women-owned enterprises. This research recommends Small and Medium Enterprises Development Authority (SMEDA), policymakers and practitioners to encourage women entrepreneurs to run their businesses for the long term by providing a variety of incentives and supports related to those internal and external factors. Numerous studies have been conducted to test the different factors’ effects on women's entrepreneurial success, but our study investigated some psychological, cultural and religious factors that are still almost untouched especially in Pakistan. The current study also contributes to the existing literature through empirical shreds of evidence.
Plasmonic chiral metasurface absorber based on bilayer fourfold twisted semicircle nanostructure at optical frequency
Authors: Cheng Yongzhi , Chen Fu , Luo Hui 
Published: December 2020

Abstract
In this paper, we present a plasmonic chiral metasurface absorber (CMSA), which can achieve the high selective absorption for right-handed and left-handed circular polarization (RCP, “+”, and LCP, “-”) lights at optical frequency. The CMSA is composed of a dielectric substrate sandwiched with bi-layer fourfold twisted semicircle metal nanostructure. The proposed CMSA has a strong selective absorption band, where absorption peaks for LCP and RCP lights occur at different resonance frequencies, reflecting the existence of a significant circular dichroism (CD) effect. It is shown that the absorbance of the CMSA can reach to 93.2% for LCP light and 91.6% for RCP light, and that the maximum CD magnitude is up to 0.85 and 0.91 around 288.5 THz and 404 THz, respectively. The mechanism of the strong chiroptical response of the CMSA is illustrated by electric fields distributions of the unit-cell nanostructure. Furthermore, the influence of the geometry of the proposed CMSA on the circular polarization selective absorption characterization is studied systematically.
In situ acoustic analysis of two 20th-century heritage carillons
Authors: Orr Scott Allan 
Published: December 2020

Abstract
Carillons are a diverse and global form of musical and civic heritage: musical instruments comprised of a series of 23 or more bells, typically hung in a tower-like structure, tuned chromatically and played from a touch-sensitive manual and pedal console using an elaborate mechanical action. Carillon bells have a distinct series of musical overtones which should be accurately tuned to one another and with other bells they sound alongside. Although these overtones have been previously studied ex situ, this study assesses the acoustic characteristics of two early-20th century carillons in Toronto, Canada as a combination of structure, bells, and mechanical action. Thus, the instrument and its context are considered holistically, more accurately reflecting the musical sensitivity of a carillonist. Spectral analysis of audio samples of each bell at different musical dynamic levels enabled the analysis of the acoustic qualities of the bells and the mechanical action of the instruments. The tuning of bells in the instruments varied; most importantly, there was a significant difference between the audial intensity of the bell tones produced by the instruments, demonstrating the importance of the mechanical action as part of the 'carillon system'. This was represented with a resistive power-law model, that represents the sensitivity of intensity to carillonist musical dynamic level. A discussion of the implications for artistic and heritage practice follows. Understanding the in situ physical acoustics of the carillon as a holistic instrument in its context informs performers, arrangers, and composers of how they can best embrace the instrument's unique qualities to improve artistic pursuits and support the appreciation of carillons as heritage instruments and function as civic voices.
A nomogram with lymph node status predicts survival and the benefit of postoperative&nbsp;&nbsp;adjuvant therapy in&nbsp;IIIA-N2&nbsp;Non-small cell lung cancer&nbsp;patients after surgery
Authors: Chen Ye , Dong Lei , Li Minjing , He Fei , Qiu ChenHui , Cai Wanru 
Published: December 2020

Abstract
Objective: This study aimed at establishing a novel nomogram predicting overall survival and investigating the survival benefit of various postoperative adjuvant treatments (POAT) in IIIA-N2 Non-small cell lung cancer (NSCLC) patients after surgery.Methods: Data of IIIA-N2 NSCLC patients between 2004 and 2016 were collected from the Surveillance, Epidemiology, and End Results (SEER). Patients were excluded if the information regarding follow-up time and clinicopathological features were incomplete. Through Univariate and multivariate analyses, independent prognostic factors were identified and integrated into the construction of nomogram. The survival benefit of POAT was evaluated in model-defined low-risk, intermediate-risk, and high-risk subgroups, respectively.Results: In total, 4389 patients were finally included for analysis. Patients’ age, sex, T stage, differentiation grade, examined lymph nodes number (ELN), metastatic lymph nodes number (MLN), and metastatic lymph nodes ratio (LNR) were identified as independent prognostic factors and were integrated into the construction of nomogram. The C-index and calibration curves indicated that the predictive performance of the nomogram was satisfactory. Patients were then categorized into three prognostic groups with the increasing risk of all-cause of death. The prognosis of patients receiving POAT (POCT or PORT plus POCT) and patients receiving surgery alone was comparable in low-risk group, while POCT could significantly prolong survival for IIIA-N2 NSCLC patients after surgery in moderate-risk and high-risk groups. Only patients in high-risk group could benefit from the combination of postoperative radiotherapy (PORT) and postoperative chemotherapy (POCT).Conclusion: In this large-cohort retrospective study, A survival-predicting nomogram and risk stratification model were established to estimate prognosis in IIIA-N2 NSCLC patients. Surgery alone was recommended as the first choice of treatment to patients in low-risk group. POCT was recommended for patients in moderate-risk group, and the combination of PORT and POCT was recommended for patients in high-risk group. This study may provide additional integration, introspection, and improvement for therapeutic decision-making.
‘SOCIETAS LEONINA’ W POGLĄDACH NIDERLANDZKIEJ I NIEMIECKIEJ JURYSPRUDENCJI OKRESU HUMANIZMU
Authors: Palmirski Tomasz 
Published: December 2020

Wieki XVI oraz XVII to dla prawa rzymskiego okres humanizmu. Działający wówczas prawnicy określani byli mianem tak zwanej eleganckiej jurysprudencji. Przedstawicielami tego nurtu w Niderlandach byli: Vinnius (Arnold Vinnius), Hubertus Giphanius, Matthäus Wesenbeck (Wesenbecius), Johannes Voet, Ulrik (Ulricus) Huber oraz Gerard Noodt. Natomiast do grona niemieckich prawników-humanistów zaliczali się, między innymi, Johann Ortwin Westenberg, Oswald Hilliger oraz Georg Frantzke.
W niniejszym artykule zostaną przedstawione ich wypowiedzi na temat societas leonina. Wynika z nich, że wzorem prawa rzymskiego przejęli oni nieważność tego typu umowy. Poza ogólnikowe stwierdzenie o nieważności spółki w przypadku opisanym w D.17,2,29,2 (jeden wspólnik otrzymuje cały zysk wypracowany przez spółkę, drugi partycypuje w całej stracie) nie wychodzą Ulrik Huber, Gerard Noodt oraz Johann Ortwin Westenberg. Podobnie Oswald Hilliger, który jednak na marginesie swoich rozważań przedstawia nowatorski pogląd Antonio Gomeza, zgodnie z którym nie cała umowa (lwiej) spółki miałaby być nieważna, lecz tylko porozumienie co do lwich udziałów. Natomiast dla Vinniusa, Giphaniusa, Wesenbecka oraz Voeta istota spółki zasadzająca się na równych udziałach (D.17,2,29 pr.) może w pewnym zakresie zostać zmieniona wolą stron. Z kolei dla Georga Frantzke w przypadku określanym jako societas leonina, zamiast kontraktu spółki, który nie może wówczas mieć miejsca (ze względu na wyłączenie jednego ze wspólników od udziału w zysku), dochodzi do darowizny, która nie rodzi żadnej skargi.
KOMISJE PRAWA W PAŃSTWACH WSPÓLNOTY NARODÓW. ZARYS ZAGADNIEŃ USTROJOWYCH
Authors: Gubała Marcin 
Published: December 2020

Artykuł przedstawia zarys zasad funkcjonowania komisji prawa w państwach Wspólnoty Narodów. Komisje są instytucjami, które działają na rzecz systematycznej reformy prawa, obejmującej cały system prawny państwa.
Opracowanie koncentruje się na zagadnieniach systemowych, takich jak sposób ustanawiania komisji, niezależność komisji i zakres powierzonych zadań. Artykuł przedstawia sposób, w jaki komisje są unormowane w aktach prawnych, oraz omawia takie zagadnienia, jak ich niezależność, przejawiająca się w oddzieleniu od innych władz, a także skład komisji, gwarantujący jej wysoki poziom intelektualny i profesjonalizm. Wśród zadań komisji wyróżniono: dokonywanie systematycznego przeglądu aktów prawnych, proponowanie zmian w poszczególnych obszarach prawa, uchylanie zbędnych i przestarzałych przepisów z systemu prawnego, jak również konsolidację i kodyfikację prawa.