bluuebunny/crossref_metadata_2025_split huggingface dataset:
The role of CENPO expression in the progression of non-small cell lung cancer
Authors: Yang Yanlong , Chen Xiaobo , Sun Hongwen , Yu Qinghe , Yin Fang , Hu Zaoxiu , Sun Yongmeng , Pu Lisha , Zhu Xinming , Li Shen , Zhao Yunping 
Published: September 2020

Abstract
Background: The role of Centromere protein O (CENPO) in the development of non-small cell lung cancer (NSCLC) is unknown. This study aimed to investigate the potential roleof CENPO in the development of NSCLCMethods: The expression level of CENPO was investigated in both tissues and cell-lines. Celigo cell counting assay, wound-healing assay, Transwell assay and Flow cytometry was used to explore the effect of CENPO on proliferation, migration, invasion, apoptosis and cell cycle of NSCLC. The potential mechanism of CENPO was explored by Human Apoptosis Antibody, also, western blot was conducted to detect the expression of PI3K/Akt/mTOR pathway and cell-cycle related protein (mTOR, P-mTOR, CDK1, CDK6 and PIK3CA). Besides, the effect of CENPO on the growth of NSCLC solid tumors was demonstrated in vivo.Results: Our study suggested CENPO gene overexpression in NSCLC. Reduced CENPO expression substantially decreased the proliferation, migration and invasion ability, and promoting apoptosis and induces cell cycle arrest of NSCLC cell-lines. Preliminary mechanism research suggested reduced CENPO could active apoptosis pathway, suppressing PI3K/AKT/mTOR pathway and down-regulation cell-cycle related proteins.Conclusion: CENPO was up-regulated in NSCLC and played an important role in promoting the progress of NSCLC.
Prevalence and Correlates of Maternal Early Stimulation Behaviors during Pregnancy in Northern Ghana: A Cross-Sectional Survey
Authors: Mackness Jessica , Gallis John A , Owusu Raymond Kofi , Ali Mohammed , Abubakr-Bibilazu Safiyatu , Adam Haliq , Aborigo Raymond , Awoonor-Williams John Koku , Lillie Margaret , McEwan Elena , Hembling John , Vasudevan Lavanya , Baumgartner Joy Noel 
Published: September 2020

Abstract
Background: Per UNICEF’s Nurturing Care Framework, early child development (ECD) begins during pregnancy and many lower-resource settings need data to inform their programs for optimal child development. The maternal-fetal relationship can be partly examined via a series of bonding activities called early stimulation behaviors (ESB). This study describes early stimulation behaviors and the associated correlates among pregnant women in Ghana. Methods: This cross-sectional study used data from a cluster randomized trial in two districts of Northern Ghana. A total of 374 pregnant women were enrolled at baseline and administered a pre-intervention survey. Communication-related early stimulation behaviors were the primary outcome and was evaluated using three maternal-fetal bonding activities; did the woman self-report touching and/or talking, singing, and talking to her belly about family. A generalized estimating equation modified Poisson model was used for the bivariate and multivariable analysis.Results: About half of the participants reported performing communication-related ESB during pregnancy frequently or sometimes. Bivariate analysis revealed that negative life experiences including higher rates of emotional, physical and sexual intimate partner violence (IPV) and having moderate to severe depressive symptoms were associated with women performing early stimulation behaviors more often. In the multivariable model, physical intimate partner violence remained significantly associated with early stimulation behaviors. Conclusion: Research on early stimulation behaviors is still in a nascent phase. It is unclear why our results revealed an association between intimate partner violence and early stimulation behaviors; this could reflect a coping mechanism for the expectant mother. Further research is needed to better understand this association and explore potential long-term impacts of early stimulation behaviors during pregnancy on child development.Trial registration: Clinical Trials # NCT03665246, September 11, 2018
Sleeping Habits Amongst Riyadh Children: A Cross-Sectional and Validation Study
Authors: Al-Khudhairy May Wathiq , Al-Sufyani Sarah M , Al-Otaibi Hadeel T , Mahjoub Randa A , Al-Shehri Asma H 
Published: September 2020

Abstract
Background: To validate the Arabic translation of the Children’s Sleep Habit Questionnaire and determine the behavioral prevalence of sleep habits amongst school aged children in Riyadh, Saudi Arabia. Methods: This prospective cross sectional study involved a Linguistic validation involving 50 bilingual parent participants in each of the Arabic and English Surveys, with a cross sectional pilot involving 1546 parent participants regarding their children’s Sleeping Habits. The Children’s Sleep Habit Questionnaire was distributed via Survey Monkey and subjected to IBM-SPSS statistics. Parents of 1546 school aged children ranging from 3 years to 12 years of age completed the survey.Results: The Total score of all domains ranged between 11 and 70. Mean Score of 35-37 in 11 % of this population. The Children’s Sleep Habit Questionnaire scores were High in comparison to the Owen’s article; greater research is needed to determine the underlying causes of the increased likelihood of sleep disorders.Conclusion: Our findings, especially in sleep anxiety are alarming and warrant for more research to determine underlying causes of the more prevalent sleep disorders (Sleep Anxiety) and any other apparent or possible causes for sleep disturbances amongst children.
Efficacy and Safety of Insulin Glargine in Patients With Acute Stroke and Hyperglycemia Receiving Intensive Care: Randomized Controlled Study
Authors: Tang Sung-Chun , Shih Shyang-Rong , Lin Shin-Yi , Chen Chih-Hao , Yeh Shin-Joe , Tsai Li-Kai , Yang Wei-Shiung , Jeng Jiann-Shing 
Published: September 2020

Abstract
Background: This pilot study compared the basal–bolus regimens of long-acting insulin glargine (IG) and neutral protamine Hagedorn (NPH) insulin in efficacy and safety in acute stroke patients with hyperglycemia receiving intensive care (NCT02607943).Methods: This was a randomized, open-label, clinical trial. Stroke patients who were admitted to the intensive care unit within 72 h of onset and met the inclusion criteria were enrolled. They received either IG or NPH with added short-acting prandial regular insulin over a 72-h period. The primary endpoints were the percentage of glucose within the range of 80 to 180 mg/dL and the percentage of glucose reduction compared with pre-randomization glucose levels, assessed through continuous glucose monitoring (CGM).Results: A total of 50 patients were included, 26 and 24 were randomly assigned to the IG and NPH, respectively. The participant baseline characteristics were comparable between groups, except the IG had a significantly higher glucose level pre-randomization than the NPH (290.69 ± 82.31 versus 246.04 ± 41.76 mg/dL, P = .021). CGM data showed that the percentage of time with glucose levels between 80 and 180 mg/dL was 45.88 ± 27.04% in the IG and 53.56 ± 22.89% in the NPH (P = .341) and the percentage of glucose reduction was 31.47 ± 17.52% in the IG and 27.28 ± 14.56% in the NPH (P = .374). The percentage of time with hypoglycemia (< 60 mg/dl) was 0.14 ± 0.49% in the IG and 0.47 ± 1.74% in the NPH (P = .361). Parameters representative of glucose variabilities, and poststroke outcomes were not significantly different between the groups.Conclusions: Our study results suggest that early initiation of an IG-based basal–bolus regimen is safe and equally effective as an NPH-based basal-bolus regimen for patients with acute stroke and hyperglycemia requiring intensive care.Trial registration: ClinicalTrials.gov, NCT02607943. Registered 18 Nov 2015, https://clinicaltrials.gov/ct2/show/NCT02607943
Barriers and Challenges Affecting Parents Use of Adrenaline Auto-Injector in Children at Risk of Anaphylaxis
Authors: Elghoudi Ahmed , Dhaheri Klithem Al , Narchi Hassib 
Published: September 2020

Abstract
Background: Anaphylaxis is a life-threatening condition. As accidental exposure to the offending allergens usually happens in the community, in absence of medical personnel, adrenaline auto-injector (AAI), carried by parents, remains the first and only drug of choice. As studies have shown that AAI is commonly underused by parents, this study was undertaken to elucidate the causes of its underutilization in our community.Methods: A cohort of parents attending the paediatric allergy clinic at Al Ain hospital, in the United Arab Emirates (UAE), completed a questionnaire survey aimed to assess their understanding and knowledge of their child’s allergy management, including their aptitude with the use the AAI, as well as their competence and comfort to provide this treatment in an emergency. Results: A total of 47 parents, 83% of whom were Emirati, including 66% of mothers, participated in the study. Food allergy was the main indication for AAI prescription, with tree nuts 62%) and peanuts (38%) being the main culprits. The majority (94%) of parents were trained by a doctor on using the AAI, with most (79%) demonstrating a good knowledge of indications for administering the AAI. Although all parents expressed satisfaction with the training that they had received, they still admitted to a remaining lack of confidence with using an AAI. Conclusions: The continuous education and training of parents on how to use an AAI, still requires supplementary psychological support to overcome their fear and anxiety with using the device in an urgent situation. More research is needed to explore the reasons behind their fear and anxiety to plan effective interventions.
Drug-Induced Cell Viability Prediction from LINCS-L1000 through WRFEN-XGBoost Algorithm
Authors: Lu Jiaxing , Chen Ming , Qin Yufang 
Published: September 2020

Abstract
BackgroundDifferent cell lines have different perturbation signatures under the action of specific compounds. Based on these perturbation signatures, it is very important to predict the cell viability and explore the biological molecular mechanism of action hidden under the phenotype. In the process of testing drug responses to the cancer, traditional experimental methods have been greatly hampered by the cost and sample size. At present, the public availability of large amounts of gene expression data makes it a challenging task to use machine learning methods to predict the drug sensitivity.ResultsIn this study, we developed the WRFEN-XGBoost cell viability prediction algorithm based on the LINCS-L1000 perturbation signatures. We integrateed the LINCS-L1000, CTRP and Achilles datasets and adopted a weighted fusion algorithm based on random forest and elastic net for key gene selection. Then, we combined the FEBPSO-XGBoost algorithm to predict the cell viability under the drug induction. Compared to other methods, our model achieved good results with 0.83 Pearson correlation. At the same time, we completed the drug sensitivity validation on the NCI60 and CCLE datasets, which further demonstrated the effectiveness of our method. ConclusionsOur results indicated that the proposed method could provide help to find the effective anti-cancer drugs and contributed to the medical research.
Cryo-EM structure of an activated GPCR-G protein complex in lipid nanodiscs
Authors: Wagner Gerhard , Zhang Meng , Gui Miao , Wang Zi-Fu , Gorgulla Christoph , Yu James , Sun Zhen-Yu , Klenk Christoph , Merklinger Lisa , Morstein Lena , Hagn Franz , Plückthun Andreas , Brown Alan , Nasr Mahmoud , Wu Hao 
Published: September 2020

Abstract
G protein coupled receptors (GPCRs) are the largest superfamily of transmembrane proteins and the targets of over 30% of currently marketed pharmaceuticals. Although several structures have been solved for GPCR-G protein complexes, structural studies of the complex in a physiological lipid membrane environment are lacking. Here, we report cryo-EM structures of lipid bilayer-bound complexes of neurotensin, neurotensin receptor 1, and Gai1b1g1 protein in two conformational states, resolved to 4.1 and 4.2 Å resolution. The structures were determined in lipid bilayer without any stabilizing antibodies/nanobodies, and thus provide a native-like platform for understanding the structural basis of GPCR-G protein complex formation. Our structures reveal an extended network of protein-protein interactions at the GPCR-G protein interface compared to in detergent micelles, defining roles for the lipid membrane in modulating the structure and dynamics of complex formation, and providing a molecular explanation for the stronger interaction between GPCR and G protein in lipid bilayers. We propose a detailed allosteric mechanism for GDP release, providing new insights into the activation of G proteins for downstream signaling.
The Epidemiological and Spatio-temporal Characteristics of 2019 Novel Coronavirus Diseases (COVID-19) in Libya
Authors: Daw Mohamed A , El-Bouzedi Abdallah H , Ahmed Mohamed O 
Published: September 2020

Abstract
Background: COVID-19 is a global pandemic that affected all aspects of life. Studies on understanding the geographical and epidemiological characteristics become particularly important in controlling the spread of the pandemic. Such studies are lacking in Northern African countries particularly in Libya, which considered being, the second largest country in Africa with the longest coast facing Europe. The objectives of this study are to; determine the epidemiological parameters and spatiotemporal patterns of COVID-19 in this large country and outline the needed strategies to contain the spread and the consequences of the pandemic. Methods: This comprehensive study included all the confirmed cases of COVID-19 since its emergence in Libya, from early April 2020 until August 31th. The epidemiological characteristics of COVID-19 were analyzed and the spatial dynamic trends were explored. A region -based counts of weekly reported cases were used to characterize and quantify the spatial dynamics of COVID-19. Results: A total 3695 of confirmed cases of OVID-19 were collected, 2515(68.1%) were males and 1180(31.9%) female with a male-to-female(M:F) ratio 2.1:1. Aged between 2 -78 years old. Older age patients infected with COVID-19 are at higher severity and mortality. A broad geographic variability and Spatiotemporal spread variation of the COVID-19 pandemic in Libya was observed. Indicating a higher significant increase of COVID-19 from the middle of July particularly in the West and Southern regions although it was consistently observed in Meddle and Southern regions. Conclusion: Evaluating the epidemiological data and the spatiotemporal dynamic trends of COVID-19 at early stages are particularly important in understanding the pandemic spread. These parameters are essential in designing effective prevention and control programs aimed at reducing the impact of COVID- 19 pandemic particularly in countries with limited resources.
Cobalt-Catalyzed Diastereo- and Enantioselective Allyl Addition to Aldehydes and α-Ketoesters through Allylic C–H Functionalization
Authors: Zhang Haiyan , Huang Jun , Meng Fanke 
Published: September 2020

Abstract
Catalytic reactions that can generate nucleophilic allyl–metal intermediates directly from simple alkenes without prefunctionalization, and ones that produce various homoallylic alcohols diastereo- and enantioselectively are of great importance in organic synthesis. Transformations that accomplish these two tasks simultaneously are in high demand, particularly if the catalysts, substrates and reagents are inexpensive and easy to access. Here we report a catalytic process that chemoselective formation of nucleophilic allyl–cobalt complexes through oxidative allylic C–H cleavage of alkenes followed by site-, diastereo- and enantioselective addition to aldehydes and α-ketoesters. The enantioenriched products that are otherwise difficult to access are obtained in up to 96% yield, with >95:5 dr and 98:2 er. The cobalt-based catalyst is derived from a commercially available chiral phosphine ligand. The utility of the method is demonstrated through enantioselective formal synthesis of lithospermic acid and total synthesis of dihydrodehydrodiconiferylalcohol.
Radiation Induced Checkpoint Immunotherapy Response in Refractory Colorectal and Pancreatic Adenocarcinoma
Authors: Parikh Aparna , Szabolcs Annamaria , Allen Jill , Clark Jeffrey , Wo Jennifer , Thel Hannah , Hoyos David , Mehta Arnav , Arshad Sanya , Lieb David , Drapek Lorraine , Blaszkowsky Lawrence , Giantonio Bruce , Weekes Colin , Zhu Andrew , Goyal Lipika , Nipp Ryan , Dubois Jon , Seventer Emily van , Foreman Bronwen , Matlack Lauren , Ly Leilana , Meurer Jessica , Hacohen Nir , Ryan David , Yeap Beow , Corcoran Ryan , Greenbaum Benjamin , Ting David , Hong Theodore 
Published: September 2020

Abstract
Immune checkpoint blockade has limited efficacy in microsatellite stable (MSS) colorectal (CRC) and pancreatic (PDAC) cancer. Preclinical models have demonstrated the use of radiation to activate the innate immune response and stimulate responsiveness to immune checkpoint blockade. Here, we describe a Phase 2 trial of radiation therapy combined with combined anti-CTLA4 (ipilimumab) and anti-PD1 (nivolumab) antibodies in MSS CRC and PDAC. In the per protocol analysis disease control rate was 37% (10/27) in CRC and 29% (5/17) in PDAC with an overall response rate of 15% (4/27) and 18% (3/17), respectively. Whole exome and RNA sequencing of biopsies from 17 patients revealed low tumor mutational burden in all tumors, but a notable upregulation of interferon stimulated genes with concordant high expression of multiple repeat RNA transcripts in responders. Altogether, this study provides foundational human proof of concept of radiation with combination immune checkpoint blockade therapy in otherwise immunotherapy resistant cancers.
Circular RNA circZNF652 is overexpressed in osteoarthritis and positively regulates LPS-induced apoptosis of chondrocytes by upregulating PTEN
Authors: Yuan Xuefeng , Zhang Yingchi , Cai Cong , Liu Chaoxu , Xie Jie , Yi Chengla 
Published: September 2020

Abstract
Background
Circular RNA circZNF652 promotes LPS-induced inflammation, which contributes to the development of osteoarthritis (OA), indicating the potential involvement of CRNDE in OA. This study was carried to explore the involvement of circZNF652 in OA.
Methods
RT-qPCR was performed to analyze the expression of circZNF652 and PTEN mRNA in synovial fluid samples from 60 OA patients and 60 healthy controls. Correlations between circZNF652 and PTEN mRNA were analyzed by Pearson’s correlation coefficient. Overexpression and siRNA silencing of circZNF652 were achieved in chondrocytes, followed by performing RT-qPCR and Western blot to analyze the expression of PTEN. The role of circZNF652 and PTEN in regulating the apoptosis of chondrocytes induced by LPS was analyzed by cell apoptosis assay.
Results
We found that circZNF652 was overexpressed in OA and positively correlated with PTEN mRNA. In chondrocytes, circZNF652 overexpression increased the expression of PTEN, and circZNF652 siRNA silencing decreased the expression of PTEN. Moreover, circZNF652 and PTEN positively regulated the apoptosis of chondrocytes induced by LPS. PTEN overexpression reversed the inhibitory effects of circZNF652 siRNA silencing on cell apoptosis.
Conclusion
CircZNF652E is overexpressed in OA and positively regulates LPS-induced apoptosis of chondrocytes by upregulating PTEN.
Common Causes and Characteristics of Adverse Drug Reactions in Older Adults: A Retrospective Study
Authors: Woo Seong-Dae , Yoon Jiwon , Doo Go-Eun , Park Youjin , Lee Youngsoo , Lee So-Hee , Lee Young-Hee , Ye Young-Min 
Published: September 2020

Abstract
Background Aging populations are often accompanied by comorbidity and polypharmacy, leading to increases in adverse drug reactions (ADRs). We sought to evaluate the causes and characteristics of ADRs in older Korean adults (≥ 65 years) in comparison to younger individuals (< 65 years).Methods Of 37,523 cases reported at a Korean pharmacovigilance center from 2011 to 2018, we reviewed 18,842 ADRs of certain or probable causality on the basis of WHO-UMC criteria. Subjects exposed to major culprits were extracted from cohorts transformed to the Observational Medical Outcomes Partnership Common Data Model during the study period.Results In total, 4,152 (22.0%) ADRs were reported for 3,437 older adults (mean age, 74.6 years and 57.3% female). Tramadol (rate ratio, 1.32; 95% confidence interval [CI], 1.21–1.44; P < 0.001) and fentanyl (1.49, 1.16–1.92, P = 0.002) posed higher risks of ADRs in the older adults, whereas nonsteroidal anti-inflammatory drugs (NSAIDs) (0.35, 0.30–0.40, P < 0.001) and iodinated contrast media (ICM) (0.82, 0.76–0.89, P < 0.001) posed lower risks. Ratios of serious ADRs to NSAIDs (odds ratio, 2.16; 95% CI, 1.48–3.15; P < 0.001) and ICM (2.09, 1.36–3.21, P = 0.001) were higher in the older adults than in the younger patients. Analgesics primarily elicited cutaneous ADRs in the younger patients and gastrointestinal reactions in the older adults. ICM more commonly led to anaphylaxis in the older adults than the younger patients (3.0% vs. 1.6%, P = 0.019).Conclusion For early detection of ADRs in older adults, better understanding of differences in the causes and characteristics thereof in comparison to the general population is needed.
Family Planning Utilization at First Sexual Initiation and Associated Factors among High School Youth Female Students in Northern Ethiopia
Authors: G/Slassie Tsega , Fisseha Girmatsion , G/Slassie Measho , Hagos Tigst 
Published: September 2020

Abstract
Background: In developing, countries young women have little awareness about safe sex practice and do casual sexual activities. The consequences such as teenage and unplanned pregnancy and birth, unsafe abortion, miscarriages, and maternal deaths due to unsafe sex practice make youth sexuality much more serious and common in developing countries. Therefore, this study aimed to assess family planning, utilization at first sexual initiation, and associated factors among high school youth female students in Alamata, Tigray, Northern Ethiopia. Methods: An institution-based cross-sectional study was employed in Alamata town, south Tigray region, Ethiopia from June 01-30, 2019. A Self-administered structured questionnaire was used to collect the data. Data were entered and analyzed using SPSS statistical package version 22. Descriptive statistics, bivariate and multivariable logistic regressions were used to assess the factor associated with family planning utilization at first sexual initiations.Results: A total of 538 youths female who initiated sexual activities with an average age of 18.2+1. 6 years participated in this study. More than half of youths 297 (55.2%) were grades 9-10 and 283 (52.6 %) were living with both their mother and father. About 122 (22.7%) of youths used family planning methods at their sexual debut. The most common methods used were emergency contraceptive pills 90 (73.8%) and male condom 32 (26.2%). For those who start sexual activities without using family planning, 42 (7.8%) experienced unwanted pregnancy. Students those who got >100birr monthly pocket money were 88% less likely to use a family planning method at first sex [AOR 0.124CI 95% (0.05-0.32)] and regards to religion students categorized as other religions were more likely to use family planning methods [AOR 1.67 CI 95% (1.17-2.57)].Conclusions: Most youth females are not using family planning at first sexual debut. This might expose them to different reproductive health problems. Therefore, an active effort to promote sexuality education and family planning use is recommended at the high school level.