bluuebunny/crossref_metadata_2025_split huggingface dataset:
Quantum dephasing induced by non-Markovian random telegraph noise
Authors: Cai Xiangji 
Published: January 2020

AbstractWe theoretically study the dynamical dephasing of a quantum two level system interacting with an environment which exhibits non-Markovian random telegraph fluctuations. The time evolution of the conditional probability of the environmental noise is governed by a generalized master equation depending on the environmental memory effect. The expression of the dephasing factor is derived exactly which is closely associated with the memory kernel in the generalized master equation for the conditional probability of the environmental noise. In terms of three important types memory kernels, we discuss the quantum dephasing dynamics of the system and the non-Markovian character exhibiting in the dynamical dephasing induced by non-Markovian random telegraph noise. We show that the dynamical dephasing of the quantum system does not always exhibit non-Markovian character which results from that the non-Markovian character in the dephasing dynamics depends both on the environmental non-Markovian character and the interaction between the system and environment. In addition, the dynamical dephasing of the quantum system can be modulated by the external modulation frequency of the environment. This result is significant to quantum information processing and helpful for further understanding non-Markovian dynamics of open quantum systems.
Establishment of sperm cryopreservation and in vitro fertilisation protocols for rats
Authors: Nakagata Naomi , Mikoda Nobuyuki , Nakao Satohiro , Nakatsukasa Ena , Takeo Toru 
Published: January 2020

AbstractRecently, genome-editing tools have come into common use in the field of rat research, and consequently, many genetically modified rat strains have been preserved and archived as frozen embryos. Although there have been many reports published on the topic of rat sperm cryopreservation, no report has yet provided satisfactory and acceptable protocols for the cryopreservation of rat sperm. In this study, we developed methods for both the cryopreservation of transgenic rat sperm and in vitro fertilisation using frozen sperm, which yielded high fertilisation rates.
High order mode structure of intense light fields generated via a laser-driven relativistic plasma aperture
Authors: Duff M. J. , Wilson R. , King M. , Gonzalez-Izquierdo B. , Higginson A. , Williamson S. D. R. , Davidson Z. E. , Capdessus R. , Booth N. , Hawkes S. , Neely D. , Gray R. J. , McKenna P. 
Published: January 2020

AbstractThe spatio-temporal and polarisation properties of intense light is important in wide-ranging topics at the forefront of extreme light-matter interactions, including ultrafast laser-driven particle acceleration, attosecond pulse generation, plasma photonics, high-field physics and laboratory astrophysics. Here, we experimentally demonstrate modifications to the polarisation and temporal properties of intense light measured at the rear of an ultrathin target foil irradiated by a relativistically intense laser pulse. The changes are shown to result from a superposition of coherent radiation, generated by a directly accelerated bipolar electron distribution, and the light transmitted due to the onset of relativistic self-induced transparency. Simulations show that the generated light has a high-order transverse electromagnetic mode structure in both the first and second laser harmonics that can evolve on intra-pulse time-scales. The mode structure and polarisation state vary with the interaction parameters, opening up the possibility of developing this approach to achieve dynamic control of structured light fields at ultrahigh intensities.
Mental models use common neural spatial structure for spatial and abstract content
Authors: Alfred Katherine L. , Connolly Andrew C. , Cetron Joshua S. , Kraemer David J. M. 
Published: January 2020

AbstractMental models provide a cognitive framework allowing for spatially organizing information while reasoning about the world. However, transitive reasoning studies often rely on perception of stimuli that contain visible spatial features, allowing the possibility that associated neural representations are specific to inherently spatial content. Here, we test the hypothesis that neural representations of mental models generated through transitive reasoning rely on a frontoparietal network irrespective of the spatial nature of the stimulus content. Content within three models ranges from expressly visuospatial to abstract. All mental models participants generated were based on inferred relationships never directly observed. Here, using multivariate representational similarity analysis, we show that patterns representative of mental models were revealed in both superior parietal lobule and anterior prefrontal cortex and converged across stimulus types. These results support the conclusion that, independent of content, transitive reasoning using mental models relies on neural mechanisms associated with spatial cognition.
Cytosine base editor 4 but not adenine base editor generates off-target mutations in mouse embryos
Authors: Lee Hye Kyung , Smith Harold E. , Liu Chengyu , Willi Michaela , Hennighausen Lothar 
Published: January 2020

AbstractDeaminase base editing has emerged as a tool to install or correct point mutations in the genomes of living cells in a wide range of organisms. However, the genome-wide off-target effects introduced by base editors in the mammalian genome have been examined in only one study. Here, we have investigated the fidelity of cytosine base editor 4 (BE4) and adenine base editors (ABE) in mouse embryos using unbiased whole-genome sequencing of a family-based trio cohort. The same sgRNA was used for BE4 and ABE. We demonstrate that BE4-edited mice carry an excess of single-nucleotide variants and deletions compared to ABE-edited mice and controls. Therefore, an optimization of cytosine base editors is required to improve its fidelity. While the remarkable fidelity of ABE has implications for a wide range of applications, the occurrence of rare aberrant C-to-T conversions at specific target sites needs to be addressed.
Exploring the formation pathways of formamide
Authors: Allen V. , van der Tak F. F. S. , López-Sepulcre A. , Sánchez-Monge Á. , Rivilla V. M. , Cesaroni R. 
Published: January 2020

Context.As a building block for amino acids, formamide (NH2CHO) is an important molecule in astrobiology and astrochemistry, but its formation path in the interstellar medium is not understood well.Aims.We aim to find empirical evidence to support the chemical relationships of formamide to HNCO and H2CO.Methods.We examine high angular resolution (~0.2″) Atacama Large Millimeter/submillimeter Array maps of six sources in three high-mass star-forming regions and compare the spatial extent, integrated emission peak position, and velocity structure of HNCO and H2CO line emission with that of NH2CHO by using moment maps. Through spectral modeling, we compare the abundances of these three species.Results.In these sources, the emission peak separation and velocity dispersion of formamide emission is most often similar to HNCO emission, while the velocity structure is generally just as similar to H2CO and HNCO (within errors). From the spectral modeling, we see that the abundances between all three of our focus species are correlated, and the relationship between NH2CHO and HNCO reproduces the previously demonstrated abundance relationship.Conclusions.In this first interferometric study, which compares two potential parent species to NH2CHO, we find that all moment maps for HNCO are more similar to NH2CHO than H2CO in one of our six sources (G24 A1). For the other five sources, the relationship between NH2CHO, HNCO, and H2CO is unclear as the different moment maps for each source are not consistently more similar to one species as opposed to the other.
A Closer Look at a Small Brain: Transnuchal Ultrasound Facilitates High-Resolution Imaging of the Cerebellum in Preterm Infants
Authors: Muehlbacher Tobias , Schaefer Rebecca Nathalie , Buss Claudia , Bührer Christoph , Schmitz Thomas 
Published: January 2020

Abstract
Purpose Very preterm infants are at risk for cerebellar injury and impaired cerebellar growth with adverse neurodevelopmental outcome. Ultrasound through the mastoid fontanel (MF) with a curved-array or sector probe is the most established method for the sonographic examination of the cerebellum. The goal of our study was to examine the validity of transnuchal ultrasound through the foramen occipitale magnum (FOM) with a linear probe for monitoring postnatal cerebellar growth.
Methods Retrospective analysis of routine ultrasound scans through FOM and MF in 105 preterm infants born between 23 and 36 weeks of gestation with a birthweight of less than 1500 g.
Results Diameters of the cerebellar hemispheres obtained through the two acoustic windows mastoid fontanel and foramen occipitale magnum showed high correlations (r’s = 0.981 and 0.983, p’s < 0.001). Corrected gestational age was significantly associated with transverse cerebellar diameter (TCD) on the first scan (r = 0.908, p < 0.001) as well as postnatal cerebellar growth (r = 0.920, p < 0.001). Postnatal growth was slightly decreased resulting in cerebellar growth restriction on serial scans. Both associations exceeded the calculated ratio of TCD to head circumference (r = 0.657, p < 0.001) and TCD to biparietal diameter with gestational age (r = 0.705, p < 0.001).
Conclusion Transnuchal ultrasound is feasible for examination of the preterm cerebellum and improves image quality compared to scans through the MF with higher resolution at a very short distance. Monitoring cerebellar growth during early postnatal life via transnuchal ultrasound can help to identify children at high risk for neurodevelopmental impairment.
Branchial Cysts in Quito, Ecuador
Authors: Pacheco-Ojeda Luis , Ayala-Ochoa Andrés , Salvador Karla 
Published: January 2020

Introduction Branchial cleft anomalies are the second most common congenital anomaly in children. However, some lesions may not develop clinically and are not diagnosed until adulthood. The recent literature of branchial cysts (BCs) in the adult population is really scanty. For this reason, we analyzed the clinical and surgical management of the adult population treated for a BC at a tertiary care general hospital.Methods A retrospective review of the clinical records of all the patients with histological diagnosis of BC who were surgically treated at the Social Security Hospital in Quito, Ecuador, was performed. Fifty-one patients (27 women) with congenital anomalies of the 2nd (43 patients with cysts) and 3rd (6 patients with cysts and 2 with fistula) branchial arches were diagnosed and treated. Diagnosis was made on clinical grounds and by computed tomography scan.Results The 43 patients with a 2nd branchial cleft cyst underwent complete surgical excision through a wide mid-neck transverse cervicotomy. The 6 cases of 3rd branchial cleft cyst underwent surgical resection through a lower-neck transverse incision, and the 2 patients with clinical fistula in the lower aspect of the neck were operated on via an elliptical incision around this external fistula opening. Postoperative evolution was uneventful in all patients.Conclusions Branchial cysts can occasionally be diagnosed in adult patients in the setting of a general hospital population. A correct clinical and imaging assessment was diagnostic in most patients. Complete surgical resection was curative in all our patients, and postoperative complications were exceptional.
Long-term Effects of Unilateral and Bilateral Otitis Media and Myringotomy on Long-Latency Verbal and Non-Verbal Auditory-Evoked Potentials
Authors: Sanfins Milaine Dominici , Donadon Caroline , Borges Letícia Reis , Skarzynski Piotr H. , Colella-Santos Maria Francisca 
Published: January 2020

Abstract
Introduction Otitis media (OM) is considered one of the most common reasons patients seek medical care in childhood. The fluctuating nature of hearing loss in cases of OM leads to irregular sound stimulation of the central auditory nervous system.
Objectives To analyze the long-latency auditory-evoked potential (LLAEP) by verbal and nonverbal sounds in children with a history of OM in the first six years of life.
Methods A total of 106 schoolchildren participated in the study, 55 females and 51 males, aged between 8 and 16 years, who were divided into 3 groups: the control group (CG), the bilateral experimental group (BEG), and the unilateral experimental group (UEG). All children underwent a complete audiological evaluation (audiometry, logoaudiometry and immitance testing) and an electrophysiological evaluation (LLAEP with toneburst stimulus – LLAEP-TB, and LLAEP with speech stimulus – LLAEP-S).
Results Both study groups (BEG and UEG) presented a statistically lower performance (p < 0.005) when compared with the CG regarding all of the electrophysiological tests with the prolongation of the latency values and decrease in the amplitude values: LLAEP-TB (BEG: latency - N1, P2, N2 [females] and P300, amplitude - N1 and P2), LLAEP-S (BEG: latency - P2 and N2 [females], amplitude - P2 /UEG: latency - P2 and P300, amplitude: N1 and P2).
Conclusion Children who had suffered secretory OM in the first six years of life and who had undergone myringotomy for the placement of a ventilation tube, either unilaterally or bilaterally, presented worse performance in their electrophysiological responses to verbal and nonverbal LLAEPs.
Placas anteriores são mais efetivas do que parafusos iliossacrais na fixação da articulação sacroilíaca? Estudo Biomecânico
Authors: Goldsztajn Flavio , Mariolani Jose Ricardo L. , Belangero William Dias 
Published: January 2020

Resumo
Introdução Usualmente, as luxações sacroilíacas são tratadas com parafusos iliossacrais ou com placas anteriores à articulação sacroilíaca (ASI). Este estudo compara a rigidez e carga máxima suportada pelos dois tipos de fixações acima citados, utilizando pelves sintéticas.
Método Dez pelves sintéticas foram divididas em dois grupos (n = 5). No grupo denominado PlaCF, a ASI foi fixada com duas placas anteriores. No grupo ParCF, a ASI foi fixada com dois parafusos iliossacrais no corpo da primeira vertebra sacral (S1). A rigidez e carga máxima suportada por cada montagem realizada, foi mensurada. A análise estatística foi realizada através do teste U de Mann-Whitney (p < 0.05 foi considerado estatisticamente significativo para todas as análises).
Resultados A carga máxima suportada até a falha da fixação pelos grupos PlaCF e ParCF foram respectivamente 940 ± 75 N e 902 ± 56 N, não havendo diferença estatística entre eles. A rigidez obtida pelo grupo ParCF foi maior e com diferença estatística em relação ao grupo PlaCF (68.6 ± 11.1 N/mm e 50 ± 4.0 N/mm respectivamente).
Conclusão Apesar da menor rigidez obtida no grupo PlaCF, as placas anteriores à ASI podem ser uma ótima opção no tratamento da luxação sacroilíaca quando os parafusos iliossacrais não puderem ser utilizados. Outros estudos são necessários para detectar possíveis diferenças entre os dois procedimentos do ponto vista cirúrgico e clínico.
Operative versus Non-Operative Management of Traumatic Thoracolumbar Injuries according to the AO Spine Thoracolumbar Spine Injury Classification System
Authors: Joaquim Andrei F. 
Published: January 2020

AbstractIntroduction The AOSpine Thoracolumbar Spine Injury Classification (AOSTSIC) system has been proposed to better characterize injury morphologies and improve the classification of thoracolumbar (TL) spine trauma. However, the indications for surgical treatment according to the AOSTSIC system are still debated. Additionally, the proposed Thoracolumbar AOSpine Injury Score (AOSIS) is quite complex, which may preclude its use in daily practice. The objective of this review is to discuss the AOSTSIC system and its indications for initial nonoperative versus surgical management of acute TL spine trauma.Methods We analyzed the literature for each injury type (and subtype, when pertinent) according to the AOSTSIC system as well as their potential treatment options.Results Patients with AOSTSIC subtypes A0, A1, and A2 are neurologically intact in the vast majority of the cases and initially managed nonoperatively. The treatment of A3- and A4-subtype injuries (burst fractures) in neurologically-intact patients is still debated with great controversy, with initially nonoperative management being considered an option in select patients. Surgery is recommended when there are neurological deficits or failure of nonoperative management, with the role of magnetic resonance findings in the Posterior Ligamentous Complex (PLC) evaluation still being considered controversial. Injuries classified as type B1 in neurologically-intact patients may be treated, initially, with nonoperative management, provided that there are no ligamentous injury and non-displacing fragments. Due to severe ligamentous injury, type-B and type-C injuries should be considered as unstable injuries that must be surgically treated, regardless of the neurological status of the patient.Conclusions Until further evidence, we provided an easy algorithm-based guide on the spinal trauma literature to help surgeons in the decision-making process for the treatment of TL spine injuries classified according to the new AOSTSIC system.
Estudo biomecânico in vitro das suturas em “oito” e de Kessler de tendões flexores de porcos
Authors: Kawachi Diogo Thomaz , Deienno Francisco Simões , Varanda Denis , Cortopassi Andrea Christina , Sardenberg Trajano 
Published: January 2020

Resumo
Objetivo Avaliar as propriedades biomecânicas dos pontos de sutura tendinosa em “oito” e de Kessler.
Métodos Tendões flexores dos dedos de membros superiores de porcos foram divididos em dois grupos com suturas triplas centrais em “oito” (seis passagens) e de Kessler (duas passagens) associadas a suturas periféricas contínuas simples, e submetidos a ensaios mecânicos longitudinais contínuos, obtendo-se as propriedades mecânicas de carga máxima e de energia na carga máxima.
Resultados As médias da carga máxima e da energia na carga máxima na sutura em “8” foram de 63,4 N e 217,3 Nmm, respectivamente; na sutura de Kessler, os valores foram de 34,19 N e 100,9 Nmm, respectivamente. A análise estatística indicou que o ponto em “oito” é superior mecanicamente ao ponto de Kessler.
Conclusões Nas condições deste experimento e no tendão flexor de dedo de membro superior de porcos, o triplo ponto em “oito” (seis passagens) é mais resistente do que o ponto de Kessler (duas passagens). O triplo ponto em “oito”, com seis passagens, permite movimentação ativa na reabilitação imediata de reparo de tendão flexor de dedo de membro superior com pouco risco de ruptura ou espaçamento na sutura.
Does A Vertebra Fracture Cause A Tumor? A Dorsolumbar Paraspinal Desmoid Tumor: Case Report
Authors: Ataizi Zeki Serdar , Simsek Gulay , Ataizi Fulya Colak , Ercan Serdar 
Published: January 2020

AbstractExtraabdominal desmoid tumors are uncommon soft-tissue tumors. The etiology of the tumor is still unclear. Injury is one of the etiological factors of soft-tissue tumors. A 41-year-old female patient who had a traumatic vertebral body fracture on the thoracic spine was treated conservatively. Two and a half years later, she presented a painful, palpable swelling on the thoracolumbar region. In the present report, was discuss the patient, who underwent a surgery to remove the desmoid tumor (aggressive fibromatosis), within the context of the current literature. The literature on desmoid tumor caused by a trauma is rare. This is the first case that demonstrates an extraabdominal desmoid tumor following a spinal fracture. The swelling on the region of the trauma must be examined carefully and desmoid tumor must be kept in mind as a possible diagnosis.
Neutralisation and contrast preservation
Authors: Bárkányi Zsuzsanna , Kiss Zoltán G. 
Published: January 2020

AbstractThis paper studies the contextual variation in the voicing properties of three-consonant clusters (CC#C) in Hungarian. We investigate the velar+alveolar stop clusters /kt/ and /ɡd/, and the alveolar fricative+stop clusters /st/ and /zd/ in potentially voicing-neutralising and assimilating contexts. We show that in these contexts, regressive voicing assimilation in Hungarian is categorical, but partially contrast preserving, and that stops and fricatives are not affected in the same way. Fricatives resist voicing before a voiced obstruent and are devoiced utterance-finally. This is a phonetically unfavourable position, therefore other duration-related cues step up to prevent complete laryngeal neutralisation.
Zfp521 SUMOylation facilities erythroid hematopoietic reconstitution under stress
Authors: Zhang Yali , Xu Shuangnian , Chen Zhe , Xie Mingling , Ma Yanni , Wu Guixian , Huang Xiangtao , Luo Chengxin , Huang Zhen , Sun Yanni , Huang Yongxiu , Li Xi , Hou Yu , Chen Jieping 
Published: January 2020

Abstract
Zinc finger protein 521 (Zfp521) is a key transcriptional factor in regulation of hematopoiesis. SUMOylation, a protein post-translational modification process, plays important roles in various biological process including hematopoiesis. However, whether Zfp521 can be SUMOylated and how it affects hematopoiesis is unknown. In this study, we confirmed that Zfp521 can be modified by SUMO1 and lysine 1146 was the primary SUMOylation site. Under homeostatic condition, Zfp521 SUMOylation-deficient mice had normal mature blood cells and primitive cells. However, in bone marrow (BM) transplantation assay, recipient mice transplanted with BM cells from Zfp521 SUMOylation-deficient mice had a significantly decreased R2 population of erythroid lineage in BM and spleen compared with those transplanted with BM cells from wild-type mice. Our results found a novel function of Zfp521 SUMOylation in erythroid reconstitution under stress, which might be a new therapeutic target in future.
Comparative analysis of enteroendocrine cells and their hormones between mouse intestinal organoids and native tissues
Authors: Ohki Junko , Sakashita Akihiko , Aihara Eitaro , Inaba Akihiko , Uchiyama Hironobu , Matsumoto Masahito , Ninomiya Yuzo , Yamane Takumi , Oishi Yuichi , Iwatsuki Ken 
Published: January 2020

Abstract
Endocrine cells in the gastrointestinal tract secrete multiple hormones to maintain homeostasis in the body. In the present study, we generated intestinal organoids from the duodenum, jejunum, and ileum of Neurogenin 3 (Ngn3)-EGFP mice and examined how enteroendocrine cells (EECs) within organoid cultures resemble native epithelial cells in the gut. Transcriptome analysis of EGFP-positive cells from Ngn3-EGFP organoids showed gene expression pattern comparable to EECs in vivo. We also compared mRNAs of five major hormones, namely, ghrelin (Ghrl), cholecystokinin (Cck), Gip, secretin (Sct), and glucagon (Gcg) in organoids and small intestine along the longitudinal axis and found that expression patterns of these hormones in organoids were similar to those in native tissues. These findings suggest that an intestinal organoid culture system can be utilized as a suitable model to study enteroendocrine cell functions in vitro.