bluuebunny/crossref_metadata_2025_split huggingface dataset:
Long non‐coding RNA FEZF1‐AS1 induced progression of ovarian cancer via regulating miR‐130a‐5p/SOX4 axis
Authors: Sun Ziqian , Gao Shouyang , Xuan Lili , Liu Xiaojun 
Published: March 2020

AbstractEmerging studies have revealed the critical role of long non‐coding RNAs (lncRNAs) in epithelial ovarian cancer (EOC) development and progression. Till now, the roles and potential mechanisms regarding FEZF1 antisense RNA 1 (FEZF1‐AS1) within ovarian cancer (OC) remain unclear. The objective of this study was to uncover the biological function and the underlying mechanism of LncRNA FEZF1‐AS1 in OC progression. FEZF1‐AS1 expression levels were studied in cell lines and tissues of human ovarian cancer. In vitro studies were performed to evaluate the impact of FEZF1‐AS1 knock‐down on the proliferation, invasion, migration and apoptosis of OC cells. Interactions of FEZF1‐AS1 and its target genes were identified by luciferase reporter assays. Our data showed overexpression of FEZF1‐AS1 in OC cell lines and tissues. Cell migration, proliferation, invasion, wound healing and colony formation were suppressed by silencing of FEZF1‐AS1. In contrast, cell apoptosis was promoted by FEZF1‐AS1 knock‐down in vitro. Furthermore, online bioinformatics analysis and tools suggested that FEZF1‐AS1 directly bound to miR‐130a‐5p and suppressed its expression. Moreover, the inhibitory effects of miR‐130a‐5p on the OC cell growth were reversed by FEZF1‐AS1 overexpression, which was associated with the increase in SOX4 expression. In conclusion, our results revealed that FEZF1‐AS1 promoted the metastasis and proliferation of OC cells by targeting miR‐130a‐5p and its downstream SOX4 expression.
Osthole stimulates bone formation, drives vascularization and retards adipogenesis to alleviate alcohol‐induced osteonecrosis of the femoral head
Authors: Yu Hongping , Zhu Daoyu , Liu Pei , Yang Qianhao , Gao Junjie , Huang Yigang , Chen Yixuan , Gao Youshui , Zhang Changqing 
Published: March 2020

AbstractCharacteristic pathological changes in osteonecrosis of the femoral head (ONFH) include reduced osteogenic differentiation of bone mesenchymal stem cells (BMSCs), impaired osseous circulation and increased intramedullary adipocytes deposition. Osthole is a bioactive derivative fromcoumarinwith a wide range of pharmacotherapeutic effects. The aim of this study was to unveil the potential protective role of osthole in alcohol‐induced ONFH. In vitro, ethanol (50 mmol/L) remarkably decreased the proliferation and osteogenic differentiation of BMSCs and impaired the proliferation and tube formation capacity of human umbilical vein endothelial cell (HUVECs), whereas it substantially promoted the adipogenic differentiation of BMSCs. However, osthole could reverse the effects of ethanol on osteogenesisviamodulating Wnt/β‐catenin pathway, stimulate vasculogenesis and counteract adipogenesis. In vivo, the protective role of osthole was confirmed in the well‐constructed rat model of ethanol‐induced ONFH, demonstrated by a cascade of radiographical and pathological investigations including micro‐CT scanning, haematoxylin‐eosin staining, TdT‐mediated dUTP nick end labelling, immunohistochemical staining and fluorochrome labelling. Taken together, for the first time, osthole was demonstrated to rescue the ethanol‐induced ONFHviapromoting bone formation, driving vascularization and retarding adipogenesis.
Length–weight relationships of mesopelagic fishes from the equatorial and tropical Atlantic waters: influence of environment and body shape
Authors: López‐Pérez Cristina , Olivar M. Pilar , Hulley Percy A. , Tuset Víctor M. 
Published: March 2020

AbstractLength–weight relationships (LWRs) were estimated for 36 mesopelagic fish species collected from the equatorial and tropical Atlantic encompassing several oceanographic regions: oligotrophic, equatorial, Cape Blanc, Cape Verde and the Canary Islands. The sample was composed of myctophids (25 species), gonostomatids (5), sternoptychids (3), stomiids (2) and phosichthyids (1). The species were clustered according to body shape: “short‐deep” (sternoptychids), “elongate” (gonostomatids, stomiids and some phosichthyids) and “fusiform” (myctophids and some phosichthyids). Three types of weight and LWRs were considered: wet weight (WW), eviscerated wet weight (eWW) and eviscerated dry weight (eDW). The study demonstrated that most species present a positive allometric growth, independent of the weight used. However, the allometric value varied in 40–50% of species depending on the type of weight considered. Significant variations linked to fish morphology were found in the relationship between the slope and intercept of the LWR equation. Significant differences were also noted in the water content linked to fish body shape. Based on the distributions of several species we compare their fitness between oceanographic regions using the relative condition factor (Krel). Except for Diaphus brachycephalus (oligotrophic vs. equatorial waters) and Lampanyctus alatus (equatorial, Cape Blanc, Cape Verde and the Canary Islands), no regional significant differences were observed in the species analysed.
Prospective evaluation of the utility of cross‐matching prior to first transfusion in cats: 101 cases
Authors: Humm K. R. , Chan D. L. 
Published: March 2020

ObjectivesTo: (1) assess the frequency of crossmatch incompatibility in naïve feline blood transfusion recipients using two crossmatching methods, (2) measure the effect of crossmatch incompatibility on change in packed cell volume following transfusion, (3) assess the frequency of acute transfusion reactions and errors in blood transfusions in cats and (4) assess the impact of crossmatch incompatibility on the likelihood of transfusion reactions.Materials and MethodsCats being administered a first AB‐matched transfusion in a veterinary teaching hospital were prospectively recruited for this observational study. A slide agglutination method and a commercial test were both used for major and minor crossmatching. We measured increase in packed cell volume at 12 hours after transfusion relative to the mass of red blood cells givenperrecipient bodyweight and recorded transfusion reactions.ResultsA total of 101 cats was included. Crossmatch incompatibility was common using the slide agglutination method (27% and 10% major and minor incompatibility, respectively), but less common with the commercial test (major and minor incompatibility both 4%). Crossmatch incompatibility with any method was not associated with less effective transfusion in terms of change in packed cell volume. Transfusion reactions occurred in 20 cats, most commonly febrile non‐haemolytic transfusion reactions (n = 9) and haemolytic transfusion reactions (n = 7). The commercial test appeared to be most specific for predicting haemolytic transfusion reactions.Clinical SignificanceTransfusion reactions were fairly common but not associated with increased mortality. Use of crossmatch‐compatible blood did not lead to a greater increase in PCV at 12 hours. The commercial test may predict a haemolytic transfusion reaction.
Cognitive impairment, quality of life and healthcare utilization in patients with chronic kidney disease stages 3 to 5
Authors: Thancharoen Onnita , Waleekhachonloet Onanong , Limwattananon Chulaporn , Anutrakulchai Sirirat 
Published: March 2020

AbstractAimThis study aimed to examine associations between cognitive impairment and quality of life and healthcare utilization in patients with chronic kidney disease (CKD) stages 3 to 5.MethodsA cross‐sectional study was conducted in 379 outpatients with a mean age of 65.7 years at tertiary care hospitals in Thailand. Cognitive function was measured using the Mini‐Mental State Examination, and quality of life was measured using the five‐dimension European quality of life (EQ‐5D‐5L) multi‐attribute utility instrument. The effects of cognitive impairment on the likelihood of reporting ‘no problems’ for each EQ‐5D dimension, the quality of life scores and healthcare utilization were determined using an appropriate multivariate analysis.ResultsThe prevalence of cognitive impairment in patients with CKD stages 3 to 5 was 15.8% (95% confidence interval [CI], 12.3, 19.9). Patients with cognitive impairment had a significantly lower likelihood of achieving good outcomes in the mobility, self‐care, usual activities and anxiety/depression dimensions of the EQ‐5D‐5L than those with normal cognition. Patients with cognitive impairment had a significantly lower quality of life score than those with normal cognition by 0.06 points (95% CI, 0.01, 0.10). Cognitive impairment increased the number of emergency visits (rate ratio, RR, 3.47; 95% CI, 1.45, 8.29). Compared to CKD stage 3, CKD stage 5 decreased the quality of life score by 0.06 points (95% CI, 0.01, 0.10) and increased the rate of hospitalization (RR, 2.29; 95% CI, 1.27, 4.12).ConclusionCognitive impairment in patients with CKD was associated with lower quality of life scores and increased healthcare utilization.
Stress‐related changes in financial risk taking: Considering joint effects of cortisol and affect
Authors: von Helversen Bettina , Rieskamp Jörg 
Published: March 2020

AbstractMany decisions under risk and uncertainty are made under physical or emotional stress. A recent meta‐analysis suggested that stress reliably influences risk taking but did not find a relation between single measures of stress such as cortisol and risk taking. One reason for the conflicting findings could be that the influence of stress on risk taking depends not only on physiological but also on psychological stress responses, in particular affective valence. We tested this hypothesis in an exploratory empirical study: Seventy participants worked on a financial risk‐taking task. In half of the participants acute stress was induced with a cold pressor task. For all participants we measured cortisol and α‐amylase levels, blood pressure, subjective arousal, and affective valence before and after the task. The stress induction increased participants' levels of cortisol, subjective arousal, and systolic blood pressure but did not directly influence negative affect or risky decision making. Examining the interplay between physiological and psychological stress responses, a moderation analysis revealed an interaction between stress induction and affect valence: Negative affect predicted an increase in risk‐seeking decision making in the stress condition, but not in the control group. A similar moderation was found with cortisol reactivity, that is, negative affect predicted an increase in risk‐seeking decision making in participants with high cortisol reactivity but not in participants with low cortisol reactivity. These results suggest that the effect of stress on risky decision making depends on the interplay of affective valence and cortisol reactivity.
Elevated body mass index during pregnancy and gestational weight gain in HIV‐infected and HIV‐uninfected women in Cape Town, South Africa: association with adverse birth outcomes
Authors: Madlala Hlengiwe P. , Malaba Thokozile R. , Newell Marie‐Louise , Myer Landon 
Published: March 2020

AbstractObjectivesTo examine the association between maternal body mass index (BMI) and gestational weight gain (GWG) and adverse birth outcomes in HIV‐infected and HIV‐uninfected women.MethodsIn an urban South African community, 2921 consecutive HIV‐infected and HIV‐uninfected pregnant women attending primary healthcare services were assessed at their first antenatal visit. A subset of HIV‐infected women enrolled in a longitudinal study was assessed three times during pregnancy. All women had birth outcome data from medical records and study questionnaires. In analyses, the associations between BMI, GWG, maternal factors and adverse birth outcomes were assessed with logistic regression models.ResultsThe estimated pre‐pregnancy BMI median was 29 kg/m2 (IQR, 24–34) overall, 29 kg/m2 (IQR, 24–34) for HIV‐uninfected and 28 kg/m2 (IQR, 24–34) for HIV‐infected women; HIV prevalence was 38%. In adjusted models, increased BMI in the overall cohort was positively associated with age, haemoglobin and parity at first antenatal visit. Maternal obesity was associated with increased likelihood of having high birthweight (aOR 2.54, 95% CI 1.39–4.66) and large size for gestational age (aOR 1.66, 95% CI 1.20–2.31) infants. In the subset cohort, GWG was associated with increased likelihood of spontaneous preterm delivery (aOR 4.35, 95% CI 1.55–12.21) and high birthweight (aOR 3.00, 95% CI 1.22–7.34) infants.ConclusionObesity during pregnancy is prevalent in this setting and appears associated with increased risk of adverse birth outcomes in both HIV‐infected and HIV‐uninfected women. Weight management interventions targeting women of child‐bearing age are needed to promote healthy pregnancies and reduce adverse birth outcomes.
Evaluation of a point‐of‐care method for screening blood donors for sickle cell status
Authors: Badawi Maha , Garoot Sara , Tahlawi Mofida , Hindawi Salwa , Adam Soheir 
Published: March 2020

BACKGROUNDTurbidity tests are commonly used for screening blood units for the presence of sickle cell trait (SCT) before transfusion to specific patient populations, based on recommendations of the AABB. In this pilot study, we evaluate a new method for screening blood donors and blood units for the presence of sickle hemoglobin.STUDY DESIGN AND METHODSThis study was based at King Abdulaziz University Hospital in Jeddah, Saudi Arabia. Study participants were approached consecutively between July 24, 2016, and August 8, 2016. Blood donors, control individuals, and known patients with sickle cell disease (SCD) were tested using both a point‐of‐care testing technology (Sickle SCAN, Biomedomics, Inc.) and hemoglobin capillary electrophoresis (HEP). Corresponding blood units were also tested using Sickle SCAN.RESULTSA total of 200 donors, 13 blood units, and 57 patients and controls were included. Sensitivity and specificity of Sickle SCAN for detection of SCT and SCD was 100%, when compared to HEP as the gold standard.CONCLUSIONSickle SCAN is a rapid test that shows high sensitivity and specificity for identification of hemoglobin S among blood donors and when used for testing blood units.
Transfusion‐associated adverse events and implementation of blood safety measures ‐ findings from the 2017 National Blood Collection and Utilization Survey
Authors: Savinkina Alexandra A. , Haass Kathryn A. , Sapiano Mathew R. P. , Henry Richard A. , Berger James J. , Basavaraju Sridhar V. , Jones Jefferson M. 
Published: March 2020

BACKGROUNDSerious transfusion‐associated adverse events are rare in the United States. To enhance blood safety, various measures have been developed. With use of data from the 2017 National Blood Collection and Utilization Survey (NBCUS), we describe the rate of transfusion‐associated adverse events and the implementation of specific blood safety measures.STUDY DESIGN AND METHODSData from the 2017 NBCUS were used with comparison to already published estimates from 2015. Survey weighting and imputation were used to obtain national estimates of transfusion‐associated adverse events, and the number of units treated with pathogen reduction technology (PRT), screened for Babesia, and leukoreduced.RESULTSThe rate of transfusion‐associated adverse events requiring any diagnostic or therapeutic interventions was stable (275 reactions per 100,000 transfusions in 2015 and 282 reactions per 100,000 transfusions in 2017). In 2017 among US blood collection centers, 16 of 141 (11.3%) reported screening units for Babesia and 28 of 144 (19.4%) reported PRT implementation; 138 of 2279 (6.1%) hospitals reported transfusing PRT‐treated platelets. In 2017, 134 of 2336 (5.7%) hospitals reported performing secondary bacterial testing of platelets (50,922 culture‐based and 63,220 rapid immunoassay tests); in 2015, 71 of 1877 (3.8%) hospitals performed secondary testing (87,155 culture‐based and 21,779 rapid immunoassay tests). Nearly all whole blood/red blood cell units and platelet units were leukoreduced.CONCLUSIONSBesides leukoreduction, implementation of most blood safety measures reported in this study remains low. Nationally, hospitals might be shifting from culture‐based secondary bacterial testing to rapid immunoassays.
Definitive‐intent radiotherapy for sinonasal carcinoma in cats: A multicenter retrospective assessment
Authors: Stiborova Katerina , Meier Valeria S. , Takada Marilia , Turek Michelle , Poirier Valerie J. , Laliberte Sarah , Rohrer Bley Carla 
Published: March 2020

AbstractTreatment of epithelial sinonasal tumours in cats is not commonly reported. In the newer reports, palliative radiation protocols have been described more often than definitive‐intent protocols. In this multi‐institutional retrospective study, we included 27 cats treated with single‐modality radiotherapy. Cats were irradiated using 10 daily fractions of 4.2 Gy. Three cats (11.1%) experienced a complete clinical response and 17 (63%) had a partial clinical response. Stable clinical disease was noted in three cats (11.1%). Four cats (14.8%) showed progression within 3 months following treatment. The median time to progression for all cases was 269 days (95 % confidence intervals [CI]: 225; 314). The proportion of cats free of progression at 1 and 2 years was 24% (95% CI: 22%; 26%) and 5% (95% CI: 5%; 6%), respectively. None of the prognostic factors evaluated were predictive of outcome (anaemia, tumour volume at the time of staging, modified Adams stage, intracranial involvement, facial deformity, epistaxis, inappetence or weight loss). Median overall survival (OS) for all deaths was 452 days (95% CI: 334; 571). The proportion of cats alive at 1 and 2 years was 57% (95% CI: 37%; 77%) and 27% (95% CI: 25%; 29%), respectively. Surprisingly, cats with epistaxis had a longer median OS of 828 days (95% CI: 356; 1301) compared to 296 days (95% CI: 85; 508) in cats without epistaxis, (P= .04, Breslow). Radiation therapy used as a single modality for the treatment of feline sinonasal carcinoma improved clinical signs and was well tolerated but progression within a year was common.
Remote liver ischaemic preconditioning protects rat brain against cerebral ischaemia–reperfusion injury by activation of an AKT‐dependent pathway
Authors: Yang Guang , Yang Yu , Li Yanmei , Hu Zhaoyang 
Published: March 2020

New Findings
What is the central question of this study?

Can remote liver ischaemic preconditioning (RLIPC) protect rat brain against cerebral ischaemia–reperfusion injury?
What is the main finding and its importance?
Pretreatment with RLIPC reduced cerebral infarct volume, improved neurological outcomes and inhibited neuron apoptosis. RLIPC led to increased phosphorylation of AKT, while inhibition of AKT abolished the effects of RLIPC. Our data suggest that liver ischaemic preconditioning exerts a strong neuroprotective effect against cerebral ischaemia–reperfusion injury by activating an AKT‐dependent pathway.
AbstractRemote limb ischaemic preconditioning has been shown to have beneficial effects in protecting brains against ischaemia–reperfusion (I/R) injury. However, little is known regarding the effect of remote liver ischaemic conditioning (RLIPC). We therefore investigated the effect of RLIPC on brain tissues suffering from I/R injury. Rats were randomly assigned to a sham group, a control group or a RLIPC group. Rats in all groups except for the sham group received middle cerebral artery occlusion (MCAO) for 1 h, followed by 48 h of reperfusion. For the RLIPC rats, four cycles of 5 min of liver ischaemia (portal vein, hepatic arterial and venous trunk occlusion) with 5 min intermittent reperfusion were carried out before cerebral ischaemia. Infarct volume was assessed after 48 h of reperfusion. Blood samples were taken for serum lactate dehydrogenase (LDH) and creatine kinase‐MB (CK‐MB) tests. Morphological changes of cortical tissue and cellular apoptosis were determined. Right cortex tissues were taken for western blotting measurements. Our data demonstrate that RLIPC reduced cerebral I/R injury, decreased the volume of the MCAO‐evoked infarct region, decreased serum levels of LDH and CK‐MB, and reduced neurological deficits and apoptosis after I/R injury. Moreover, rats receiving RLIPC showed increased cortical AKT phosphorylation, but protein phosphorylation level was unchanged in the survivor activating factor enhancement (SAFE) signalling pathway. Accordingly, inhibition of AKT with wortmannin abolished the neuroprotective action of liver preconditioning. Our study showed for the first time that liver ischaemic preconditioning effectively protects brain against cerebral I/R injury by activating an AKT‐dependent pathway.
Intervention reporting of clinical trials published in high-impact cardiology journals: effect of the TIDieR checklist and guide
Authors: Palmer William , Okonya Ochije , Jellison Samuel , Horn Jarryd , Harter Zachery , Wilkett Matt , Vassar Matt 
Published: March 2020

BackgroundRandomised controlled trials (RCTs) provide the highest-level of evidence among primary research in cardiovascular medicine. Yet, even the best trial may be less useful if it fails to provide an accurate means of reproducibility. Unfortunately, discrepancies in the standards of trial reporting have been persistent in previous trials. The Template for Intervention Description and Replication (TIDieR) checklist aims to improve research efficacy by setting standards for quality intervention reporting and reproducibility. The goal of this study was to assess adherence to the TIDieR checklist among RCTs published in cardiovascular health journals. We also compared the quality of intervention reporting before and after the publication of TIDieR.MethodsThis cross-sectional, methodological study analysed 101 trials published within high-impact cardiology journals. Our primary objective was to assess overall adherence to the TIDieR checklist. Our secondary objective was to use an interrupted time-series analysis to determine if intervention reporting increased following the publication of TIDieR in March 2014. Additionally, we used generalised estimating equations to identify trial characteristics associated with intervention reporting.ResultsTrials in our sample reported 8.6/12 TIDieR checklist items, on average. The most under-reported items were those for describing the expertise of the interventionists and for describing the location of the intervention.ConclusionImproved outcome reporting and intervention reproducibility among RCTs are greatly needed in cardiovascular medicine. Clinicians and researchers should advocate for the ethical publication of complete, translatable and replicable clinical research results.
Diagnosing lymphoma in dogs
Authors: Villiers Elizabeth 
Published: March 2020

Background: In recent years, the veterinary oncology community has started to consider lymphoma as being a multitude of different diseases. Different types of lymphoma respond differently to different treatments and carry different prognoses, so it is important to identify these individual forms rather than giving a blanket diagnosis of ‘lymphoma’.Aim of the article: This article lists the various forms of lymphoma and describes the methods that are available for accurate identification of the different subtypes, which leads to better prognosis prediction and selection of the most appropriate treatment for patients.