bluuebunny/crossref_metadata_2025_split huggingface dataset:
Recipes for recombining DNA: A history ofMolecular Cloning: A Laboratory Manual
Authors: Creager Angela N.H. 
Published: December 2020

AbstractLaboratory instructions and recipes are sometimes edited into books with a wide circulation. Even in the late twentieth century, publications of this nature remained influential. For example, protocols from a 1980 summer course on gene cloning at Cold Spring Harbor Laboratory provided the basis for a bestselling laboratory manual by Tom Maniatis, Ed Fritsch and Joe Sambrook. Not only did theMolecular Cloning: A Laboratory Manualbecome a standard reference for molecular biologists (commonly called the ‘bible’), but also its recipes and clear instructions made gene cloning and recombinant DNA technologies accessible to non-specialists. Consequently, this laboratory manual contributed to the rapid spread of genetic-engineering techniques throughout the life sciences, as well as in industry. As is often the case with how-to books, however, finding a way to update methods in this rapidly changing field posed a challenge, and various molecular-biology reference books had different ways of dealing with knowledge obsolescence. This paper explores the origins of this manual, its publication history, its reception and its rivals – as well as the more recent migration of such laboratory manuals to the Internet.
The Fellow Who Made Himself President of a European Republic: Gregory Ignatius Zhatkovych
Authors: Magocsi Paul Robert 
Published: December 2020

AbstractThis is the first comprehensive biography based on unique archival sources about Gregory Ignatius Zhatkovych, a Pittsburgh-based lawyer, who in 1918–1919 was instrumental in the creation of Czechoslovakia and the inclusion of its far eastern region, Subcarpathian Rus'/Ruthenia, into the new country.
A low-frequency blind survey of the low Earth orbit environment using non-coherent passive radar with the Murchison widefield array
Authors: Prabu S. , Hancock P. , Zhang X. , Tingay S. J. 
Published: December 2020

AbstractWe have extended our previous work to use the Murchison widefield array (MWA) as a non-coherent passive radar system in the FM frequency band, using terrestrial FM transmitters to illuminate objects in low Earth orbit (LEO) and the MWA as the sensitive receiving element for the radar return. We have implemented a blind detection algorithm that searches for these reflected signals in difference images constructed using standard interferometric imaging techniques. From a large-scale survey using 20 h of archived MWA observations, we detect 74 unique objects over multiple passes, demonstrating the MWA to be a valuable addition to the global Space Domain Awareness network. We detected objects with ranges up to 977 km and as small as$0.03$${\rm m}^2$radar cross section. We found that 30 objects were either non-operational satellites or upper-stage rocket body debris. Additionally, we also detected FM reflections from Geminid meteors and aircraft flying over the MWA. Most of the detections of objects in LEO were found to lie within the parameter space predicted by previous feasibility studies, verifying the performance of the MWA for this application. We have also used our survey to characterise these reflected signals from LEO objects as a source of radio frequency interference (RFI) that corrupts astronomical observations. This has allowed us to undertake an initial analysis of the impact of this RFI on the MWA and the future square kilometer array (SKA). As part of this analysis, we show that the standard MWA RFI flagging strategy misses most of this RFI and that this should be a careful consideration for the SKA.
Screening of central obesity among normal-weight children and adolescents in Shandong, China
Authors: Zhang Ying-xiu , Chen Jian , Liu Xiao-hui 
Published: December 2020

AbstractThe prevalence of central obesity in the total population has been reported in numerous studies. However, information on the prevalence of central obesity within normal-category BMI is scant. In the present study, we examined the profiles of central obesity among normal-weight children and adolescents. A total of 29 516 (14 226 boys and 15 290 girls) normal-weight children and adolescents (excluding underweight, overweight and obesity) aged 7–18 years were included in the final analysis. Central obesity was defined by the international age- and sex-specific cut-offs of waist circumference (WC) and threshold of waist:height ratio (WHtR ≥ 0·5). All subjects were classified into four groups (Q1–Q4) according to the age- and sex-specific quartiles of BMI, those in the upper fourth (Q4) were defined as ‘high-normal BMI’ and those in the lower fourth (Q1) were defined as ‘low-normal BMI’. The prevalence of central obesity as measured by WC was 9·90 (95 % CI 9·41, 10·39) % for boys and 8·11 (95 % CI 7·68, 8·54) % for girls; by WHtR was 2·97 (95 % CI 2·69, 3·25) % for boys and 2·44 (95 % CI 2·20, 2·68) % for girls. Subjects in the Q4 group had a much higher prevalence of central obesity than their counterparts in the Q1 group (P < 0·01). Our findings suggest that the health risks of children with normal-weight central obesity may be missed when BMI is used alone as a measure; it is meaningful to include WC in clinical practice and to include the simple message ‘Keep your waist to less than half your height’.
THE POLITICS OF DANCE:EUNOMIAAND THE EXCEPTION OF DIONYSUS IN PLATO'SLAWS
Authors: Yu Kenneth W. 
Published: December 2020

How to inculcate virtue in the citizens of Magnesia by means of the dance component ofchoreiaconstitutes one of the principal concerns in theLaws(=Leg.), revealing Plato's evolving ideas about the expediency of music andpaideiafor the construction of his ideal city since theRepublic. Indeed, a steady stream of monographs and articles on theLawshas enriched our understanding of how Plato theorizes the body as a site of intervention and choral dance as instrumental in solidifying social relations and in conditioning the ethical and political self. As one scholar has aptly put it: ‘a city and its sociopolitical character [are] effectively danced into existence.’ Drawing on this recent work, I focus on an enigmatic passage inLawsBook 7 that merits more attention than it has received, in which Plato curiously singles out Bacchic dances from those that are ‘without controversy’ (815b7–d4):τὴν τοίνυν ἀμφισβητουμένην ὄρχησιν δεῖ πρῶτον χωρὶς τῆς ἀναμφισβητήτου διατεμεῖν. τίς οὖν αὕτη, καὶ πῇ δεῖ χωρὶς τέμνειν ἑκατέραν; ὅση μὲν βακχεία τ᾽ ἐστὶν καὶ τῶν ταύταις ἑπομένων, ἃς Νύμφας τε καὶ Πᾶνας καὶ Σειληνοὺς καὶ Σατύρους ἐπονομάζοντες, ὥς φασιν, μιμοῦνται κατῳνωμένους, περὶ καθαρμούς τε καὶ τελετάς τινας ἀποτελούντων, σύμπαν τοῦτο τῆς ὀρχήσεως τὸ γένος οὔθ᾽ ὡς εἰρηνικὸν οὔθ᾽ ὡς πολεμικὸν οὔθ᾽ ὅτι ποτὲ βούλεται ῥᾴδιον ἀφορίσασθαι: διορίσασθαι μήν μοι ταύτῃ δοκεῖ σχεδὸν ὀρθότατον αὐτὸ εἶναι, χωρὶς μὲν πολεμικοῦ, χωρὶς δὲ εἰρηνικοῦ θέντας, εἰπεῖν ὡς οὐκ ἔστι πολιτικὸν τοῦτο τῆς ὀρχήσεως τὸ γένος, ἐνταῦθα δὲ κείμενον ἐάσαντας κεῖσθαι, νῦν ἐπὶ τὸ πολεμικὸν ἅμα καὶ εἰρηνικὸν ὡς ἀναμφισβητήτως ἡμέτερον ὂν ἐπανιέναι.So, first of all, we should separate questionable dancing far from dancing that is without controversy. Which is the controversial kind, and how are the two to be distinguished? All the dancing that is of a Bacchic kind and cultivated by those who indulge in intoxicated imitations of Nymphs, Pans, Sileni and Satyrs (as they name them), when performing certain rites of expiation and initiation—this entire class of dancing cannot easily be marked off either as pacific or as warlike, nor as of any one particular kind. The most correct way of defining it appears to me to be this—to place it away from both pacific and warlike dancing, and to pronounce that this type of dancing is οὐ πολιτικόν; having thus set aside and dismissed it, we will now return to the warlike and pacific types, which without controversy belong to us.
Electrophysiological effects of slim straight intracochlear electrode position
Authors: Ordonez F , Sudhoff H , Todt I 
Published: December 2020

AbstractObjectiveThe electrical current distribution of a cochlear implant electrode within the cochlea is essential for post-operative hearing performance. The slim straight electrode is designed to enable the placement of contacts in a lateral or medial direction to the modiolus. The electrophysiological effect of this different contact direction is so far unknown. The aim of this study was to determine the influence of intracochlear laterally or medially directed electrode contacts on electrophysiological behaviour.MethodA slim straight electrode was inserted into the cochleae of five patients, and the neural response threshold was measured in a laterally and medially directed contact position. The cochleae in five temporal bone specimens were de-capped allowing an insertional observation of the contact position (lateral versus medial) of the electrode.ResultsThere was no difference in neural response threshold between a lateral and a medial position of the contacts. Temporal bone study indicated no intracochlear torsion of the electrode.ConclusionOur study provides evidence that the intracochlear position of slim straight electrode contacts does not affect the neural response threshold.
Time to break up with the international community? Rhetoric and realities of a political myth in Cambodia
Authors: Travouillon Katrin , Bernath Julie 
Published: December 2020

AbstractThe international community is as ubiquitous as it is elusive and its universalist pretensions remain unchallenged in political and academic discourse. In response, this article turns to Bottici's work on political myths. Against the notion of myths as falsehoods, we argue that they create their own sphere of shared social and political reality. The analysis centres on the case of Cambodia, a country that served as an experiment of liberal interventionism. It draws on archival and field research on two consecutive international interventions, a review of public statements by international actors, and interviews with Cambodian actors and activist. We argue that to understand the ideas actors use to orient themselves as they press for change, it is necessary to consider how decades of engagement with the myth have shaped the political imaginary. Our empirical analysis points to three different phases in the use of the myth: Its production during UNTAC, the reinforcement of its narratives through subsequent legal, aid and development interventions, and finally its contemporary use in a post-liberal context. We observe that Cambodian actors increasingly engage the myth to question the terms of transnational cooperation for democracy. Our work has implications for assessments of the legacies of liberal peacebuilding.
Methylation of the glucocorticoid receptor promoter in children: Links with parents as teachers, early life stress, and behavior problems
Authors: Gardini Elena Silvia , Schaub Simone , Neuhauser Alex , Ramseier Erich , Villiger Arna , Ehlert Ulrike , Lanfranchi Andrea , Turecki Gustavo 
Published: December 2020

AbstractThe present study examined the effect of early life stress (ELS) on the glucocorticoid receptor gene (NR3C1) methylation, the associations between NR3C1 methylation and behavior problems, and the effect of the program Parents as Teachers (PAT) on NR3C1 methylation. Participants included 132 children, 72 assigned to the PAT intervention group and 60 to the PAT control group. Children were aged 3 years, and were living in psychosocially at-risk families. We assessed NR3C1 methylation of the NGFI-A binding regions of exon 1F via sodium bisulfite sequencing from saliva DNA. Results indicated that (a) children living in families receiving PAT had decreased methylation at one single cytosine–guanine dinucleotides (CpG) site; (b) current maternal depressive symptoms and parental disagreement were predictive of increased methylation of mean NGFI-A and three single CpG sites; and (c) increased methylation of mean NGFI-A and one single CpG site was significantly associated with increased internalizing and externalizing symptoms. In addition, mean NGFI-A was a mediator of the association between parental disagreement and a child's affective problems. These results suggest that PAT may contribute to preventing NR3C1 methylation in preschool children living in psychosocially at-risk situations, and confirm previous findings on the associations between ELS, NR3C1 methylation, and behavior problems.
‘A mere gutter!’ The Carioca Aqueduct and water delivery in mid-nineteenth-century Rio de Janeiro
Authors: Metcalf Alida C. , Smith Sean Morey , Kennedy S. Wright 
Published: December 2020

AbstractThe two key aspects of water infrastructure – engineered and human – in mid-nineteenth-century Rio de Janeiro are the foci of this article. On the one hand, gravity flow engineering brought fresh water from the Tijuca Forest to the fountains in the city, but on the other, hundreds of slaves carried heavy jugs of water from the fountains though the streets to residences. Using the account of Thomas Ewbank (1856), georeferenced historical maps and a field study, this article first reconstructs the route of the Carioca Aqueduct, then, using the accounts of Ewbank and other travellers, turns to the delivery of water in the city by enslaved water carriers.
Vitamin C deficiency as an unusual cause of pulmonary hypertension and refusal to walk
Authors: Tan Jason W. Y. , Lee Olive P. E. , Leong Ming Chern 
Published: December 2020

AbstractVitamin C deficiency has been a historical disease rarely seen nowadays. We illustrate a case of a boy with autism presenting with severe pulmonary hypertension and refusal to walk secondary to vitamin C deficiency. Initiating treatment with high-dose vitamin C reversed his symptoms and he regained full power of his lower limbs with total normalisation of his pulmonary pressures.
Does high-variation training facilitate transfer of training in paediatric transthoracic echocardiography?
Authors: McMahon Colin J. , Gallagher Sarah , James Adam , Deery Aoife , Rhodes Mark , van Merriënboer Jeroen J. G. 
Published: December 2020

AbstractBackground:Factors that facilitate transfer of training in paediatric echocardiography remain poorly understood. This study assessed whether high-variation training facilitated successful transfer in paediatric echocardiography.Methods:A mixed-methods study of transfer of technical and interpretive skill application amongst postgraduate trainees. Trainees were randomised to a low or high-variation training group. After a period of 8 weeks intensive echocardiography training, we video-recorded how trainees completed an echocardiogram in a complex cardiac lesion not previously encountered. Blinded quantitative analysis and scoring of trainee performance (echocardiogram performance, report, and technical proficiency) were performed using a validated assessment tool by a blinded cardiologist and senior cardiac physiologist. Qualitative interviews of the trainees were recorded to ascertain trainee experiences during the training and transfer process.Results:Sixteen trainees were enrolled in the study. For the cumulative score for all three components tested (echocardiogram performance, report, and technical proficiency), χ2 = 8.223, p = .016, which showed the high-variation group outperformed the low-variation group. Two common themes which assisted in the transfer emerged from interviews are as follows: (1) use of strategies described in variation theory to describe abnormal hearts, (2) the use of formative live feedback from trainers during hands-on training.Conclusion:Training strategies exposing trainees to high-variation training may aid transfer of paediatric echocardiography skills.
Evaluation of emotional (depression) and behavioural (nutritional, physical activity and sleep) status of Turkish adults during the COVID-19 pandemic period
Authors: Karahan Yılmaz Sevil , Eskici Günay 
Published: December 2020

AbstractObjective:To evaluate emotional (depression) and behavioural (nutritional behaviours, physical activity status and sleep patterns) of Turkish adult individuals during the COVID-19 pandemic period.Design:Cross-sectional online survey. The participants filled out a questionnaire (developed by using Google Forms) that contained descriptive characteristics, nutritional behaviours, sleep patterns, physical activity status, anthropometric measurements, COVID-19-related level of knowledge and the questions of the Center for Epidemiological Studies Depression Scale through e-mail or social media (WhatsApp).Setting:Turkey.Participants:Totally, 1120 adult individuals who completed an online survey between April and May 2020.Results:It was determined that 29·1 % of the individuals showed mild, 34·2 % moderate and 23·4 % severe depression symptoms during the pandemic period. A significant relationship was found between gender, age and educational status, marital status and depression levels of the individuals, respectively (χ2= 35·292, χ2= 103·46, χ2= 24·524 and χ2= 86·208,P< 0·05). The top three foods consumed most during the pandemic period are tea and coffee (66·6 %), pastry (e.g. cake and cookie) (56·4 %) and desserts (49·6 %). During the pandemic period, 42·5 % of the individuals stated that they slept more and 40·2 % stated that there was no change in their sleep patterns. Daily physical activity durations were determined as 8·25 ± 1·77 h for sleep, 4·21 ± 2·68 h for lying down, 5·42 ± 2·64 h for sitting and 6·16 ± 4·82 h for standing activities.Conclusion:It was determined that the individuals showed different levels of depression symptoms during the pandemic period. Especially, carbohydrate food consumption increased, and physical activity status and sleep patterns changed due to the increased time spent sitting and lying.
Pneumonia in endangered aquatic mammals and the need for developing low-coverage vaccination for their management and conservation
Authors: Nabi Ghulam , McLaughlin Richard W. , Khan Suliman , Hao Yujiang , Chang Ming Xian 
Published: December 2020

AbstractAnthropogenic activities can lead to several devastating effects on the environment. The pollutants, which include the discharge of effluents, runoffs in the form of different lethal and sub-lethal concentrations of pesticides, heavy metals, and other contaminants, can harm exposed fauna and flora. The aquatic environment is the ultimate destination for many pollutants which negatively affect aquatic biodiversity and even can cause a species to become extinct. A pollutant can directly affect the behavior of an animal, disrupt cellular systems, and impair the immune system. This harm can be reduced and even mitigated by adopting proper approaches for the conservation of the target biota. Among aquatic organisms, cetaceans, such as the Yangtze finless porpoise, Irrawaddy dolphin, Ganges River dolphin, Amazon River dolphin, and Indus River dolphin, are at a higher risk of extinction because of lack of knowledge and research, and thus insufficient information with respect to their conservation status, management, and policies. Pneumonia is one of the leading causes of mass mortalities of cetaceans. This article reviews the limited research reported on stress and pneumonia induced by pollution, stress-induced pneumonia and immunosuppression, pneumonia-caused mass mortalities of aquatic mammals, and vaccination in wildlife with a specific focus on aquatic mammals, the role of genomics in vaccine development and vaccination, and the major challenges in vaccine development for biodiversity conservation.
The origins of the state: technology, cooperation and institutions
Authors: Benati Giacomo , Guerriero Carmine 
Published: December 2020

AbstractWe develop a theory of state formation shedding light on the rise of the first stable state institutions in Bronze Age Mesopotamia. Our analysis suggests that the mix of adverse production conditions and unforeseen innovations pushed groups favored by old technologies to establish the state by granting political and property rights to powerless individuals endowed with new and complementary skills. Through these reforms, the elite convinced the nonelite that a sufficient part of the returns on joint investments would be shared via public spending and, thus, to cooperate and accumulate a culture of cooperation. Different from the main alternative theories, we stress that: (1) group formation is heavily shaped by unforeseen shocks to the returns on both risk-sharing and innovation; (2) complementarity in group-specific skills, and not violence, is key determinant of state formation; (3) military, merchant and, especially, religious ranks favored state formation and culture accumulation.
Using a stochastic economic scenario generator to analyse uncertain superannuation and retirement outcomes
Authors: Chen Wen , Koo Bonsoo , Wang Yunxiao , O’Hare Colin , Langrené Nicolas , Toscas Peter , Zhu Zili 
Published: December 2020

AbstractThe retirement systems in many developed countries have been increasingly moving from defined benefit towards defined contribution system. In defined contribution systems, financial and longevity risks are shifted from pension providers to retirees. In this paper, we use a probabilistic approach to analyse the uncertainty associated with superannuation accumulation and decumulation. We apply an economic scenario generator called the Simulation of Uncertainty for Pension Analysis (SUPA) model to project uncertain future financial and economic variables. This multi-factor stochastic investment model, based on the Monte Carlo method, allows us to obtain the probability distribution of possible outcomes regarding the superannuation accumulation and decumulation phases, such as relevant percentiles. We present two examples to demonstrate the implementation of the SUPA model for the uncertainties during both phases under the current superannuation and Age Pension policy, and test two superannuation policy reforms suggested by the Grattan Institute.
Reduced peanut sensitization with maternal peanut consumption and early peanut introduction while breastfeeding
Authors: Azad Meghan B. , Dharma Christoffer , Simons Elinor , Tran Maxwell , Reyna Myrtha E. , Dai Ruixue , Becker Allan B. , Marshall Jean , Mandhane Piushkumar J. , Turvey Stuart E. , Moraes Theo J. , Lefebvre Diana L. , Subbarao Padmaja , Sears Malcolm R. 
Published: December 2020

AbstractNew guidelines for peanut allergy prevention in high-risk infants recommend introducing peanut during infancy but do not address breastfeeding or maternal peanut consumption. We assessed the independent and combined association of these factors with peanut sensitization in the general population CHILD birth cohort (N = 2759 mother–child dyads). Mothers reported peanut consumption during pregnancy, timing of first infant peanut consumption, and length of breastfeeding duration. Child peanut sensitization was determined by skin prick testing at 1, 3, and 5 years. Overall, 69% of mothers regularly consumed peanuts and 36% of infants were fed peanut in the first year (20% while breastfeeding and 16% after breastfeeding cessation). Infants who were introduced to peanut early (before 1 year) after breastfeeding cessation had a 66% reduced risk of sensitization at 5 years compared to those who were not (1.9% vs. 5.8% sensitization; aOR 0.34, 95% CI 0.14–0.68). This risk was further reduced if mothers introduced peanut early while breastfeeding and regularly consumed peanut themselves (0.3% sensitization; aOR 0.07, 0.01–0.25). In longitudinal analyses, these associations were driven by a higher odds of outgrowing early sensitization and a lower odds of late-onset sensitization. There was no apparent benefit (or harm) from maternal peanut consumption without breastfeeding. Taken together, these results suggest the combination of maternal peanut consumption and breastfeeding at the time of peanut introduction during infancy may help to decrease the risk of peanut sensitization. Mechanistic and clinical intervention studies are needed to confirm and understand this “triple exposure” hypothesis.