bluuebunny/crossref_metadata_2025_split huggingface dataset:
Accuracy for diagnosis of periapical cystic lesions
Authors: Tsesis Igor , Krepel Gal , Koren Tal , Rosen Eyal , Kfir Anda 
Published: August 2020

AbstractClinical differentiation between cystic lesions of endodontic and non-endodontic origin is of importance because correct diagnosis may affect treatment decision making. Most radicular cysts are treated with conservative approaches and, therefore, are not surgically removed. The objective of this study was to determine the accuracy of clinical diagnosis of periapical lesions as compared to the histological findings, and to evaluate various associated factors. All biopsy specimens submitted for histological evaluation from 2002 to 2009 were assessed. Only cases of periapical lesions with complete patient data and clinical diagnosis were included. Sensitivity, specificity and accuracy of the clinical diagnosis were calculated and various patient-related factors were evaluated. Of the 4,908 cases, 183 met inclusion criteria. Histologically, there were 171 lesions of radicular cysts and 12 cases of non-endodontic cysts, including OKC and Incisive Canal Cyst. The diagnostic accuracy for clinical diagnosis for radicular cysts was 91.84% and 91.84% for non-endodontic cysts. There was a high accuracy of clinical differentiation between cystic lesions of endodontic and non-endodontic origin. However, some non-endodontic lesions may be incorrectly diagnosed clinically as lesions of endodontic origin. Histological evaluation may be necessary for the correct diagnosis. Further clinical studies are needed to evaluate clinical examination and histological diagnosis of periapical lesions.
An extended logistic model of photodynamic inactivation for various levels of irradiance using the example of Streptococcus agalactiae
Authors: Brasel Michal , Pieranski Michal , Grinholc Mariusz 
Published: August 2020

AbstractIrradiance is an important factor influencing the acceleration of microorganism mortality in photodynamic inactivation (PDI) processes. Experimental observations of PDI processes indicate that the greater the irradiation power is, the faster the decrease in the population size of microorganisms. However, commonly used mathematical models of PDI processes usually refer only to specific values of irradiance without taking into account the influence of change in irradiance on the dynamic properties of inactivation. The main goal of this paper is to analyze the effect of irradiance on the PDI process and attempt to mathematically model the obtained dependencies. The analysis was carried out using the example of photodynamic inactivation of the bacterium Streptococcus agalactiae with the adopted Logistic PDI model optimized for several selected levels of irradiance. To take into account the impact of changes in irradiation power on the PDI model, the selected parameters were made appropriately dependent on this factor. The paper presents several variants of parameter modification with an evaluation of the model fitting quality criterion. The discussion on appropriate selection of parameters to be modified was carried out as a comparative analysis of several case studies. The extended logistic PDI model obtained in the conducted research effectively describes the dynamics of microorganism mortality in the whole tested irradiation power range.
Depletion of senescent-like neuronal cells alleviates cisplatin-induced peripheral neuropathy in mice
Authors: Acklin Scarlett , Zhang Manchao , Du Wuying , Zhao Xin , Plotkin Matthew , Chang Jianhui , Campisi Judith , Zhou Daohong , Xia Fen 
Published: August 2020

AbstractChemotherapy-induced peripheral neuropathy is among the most common dose-limiting adverse effects of cancer treatment, leading to dose reduction and discontinuation of life-saving chemotherapy and a permanently impaired quality of life for patients. Currently, no effective treatment or prevention is available. Senescence induced during cancer treatment has been shown to promote the adverse effects. Here, we show that cisplatin induces senescent-like neuronal cells in primary culture and in mouse dorsal root ganglia (DRG), as determined by the characteristic senescence markers including senescence-associated beta-galactosidase, accumulation of cytosolic p16INK4A and HMGB1, as well as increased expression of p16Ink4a, p21, and MMP-9. The accumulation of senescent-like neuronal cells in DRG is associated with cisplatin-induced peripheral neuropathy (CIPN) in mice. To determine if depletion of senescent-like neuronal cells may effectively mitigate CIPN, we used a pharmacological ‘senolytic’ agent, ABT263, which inhibits the anti-apoptotic proteins BCL-2 and BCL-xL and selectively kills senescent cells. Our results demonstrated that clearance of DRG senescent neuronal cells reverses CIPN, suggesting that senescent-like neurons play a role in CIPN pathogenesis. This finding was further validated using transgenic p16-3MR mice, which permit ganciclovir (GCV) to selectively kill senescent cells expressing herpes simplex virus 1 thymidine kinase (HSV-TK). We showed that CIPN was alleviated upon GCV administration to p16-3MR mice. Together, the results suggest that clearance of senescent DRG neuronal cells following platinum-based cancer treatment might be an effective therapy for the debilitating side effect of CIPN.
Increasing the broad-leaved tree fraction in European forests mitigates hot temperature extremes
Authors: Schwaab Jonas , Davin Edouard L. , Bebi Peter , Duguay-Tetzlaff Anke , Waser Lars T. , Haeni Matthias , Meier Ronny 
Published: August 2020

AbstractForests influence climate through a myriad of chemical, physical and biological processes and are an essential lever in the efforts to counter climate change. The majority of studies investigating potential climate benefits from forests have focused on forest area changes, while changes to forest management, in particular those affecting species composition, have received much less attention. Using a statistical model based on remote sensing observations over Europe, we show that broad-leaved tree species locally reduce land surface temperatures in summer compared to needle-leaved species. The summer mean cooling effect related to an increase in broad-leaved tree fraction of 80% is relatively modest (~ 0.3–0.75 K), but is amplified during exceptionally warm periods. The reduction of daily maximum temperatures during the hottest days reaches up to 1.8 K in the Atlantic region and up to 1.5 K in Continental and Mediterranean regions. Hot temperature extremes adversely affect humans and ecosystems and are expected to become more frequent in a future climate. Thus, forest management strategies aiming to increase the fraction of broad-leaved species could help to reduce some of the adverse local impacts caused by hot temperature extremes. However, the overall benefits and trade-offs related to an increase in the broad-leaved tree fraction in European forests needs to be further investigated and assessed carefully when adapting forest management strategies.
Electrical activity at the AlN/Si Interface: identifying the main origin of propagation losses in GaN-on-Si devices at microwave frequencies
Authors: Bah Micka , Valente Damien , Lesecq Marie , Defrance Nicolas , Garcia Barros Maxime , De Jaeger Jean-Claude , Frayssinet Eric , Comyn Rémi , Ngo Thi Huong , Alquier Daniel , Cordier Yvon 
Published: August 2020

AbstractAlN nucleation layers are the basement of GaN-on-Si structures grown for light-emitting diodes, high frequency telecommunication and power switching systems. In this context, our work aims to understand the origin of propagation losses in GaN-on-Si High Electron Mobility Transistors at microwaves frequencies, which are critical for efficient devices and circuits. AlN/Si structures are grown by Metalorganic Vapor Phase Epitaxy. Acceptor dopant in-diffusion (Al and Ga) into the Si substrate is studied by Secondary Ion Mass Spectroscopy and is mainly located in the first 200 nm beneath the interface. In this region, an acceptor concentration of a few 1018 cm-3 is estimated from Capacitance–Voltage (C–V) measurements while the volume hole concentration of several 1017 cm-3 is deduced from sheet resistance. Furthermore, the combination of scanning capacitance microscopy and scanning spreading resistance microscopy enables the 2D profiling of both the p-type conductive channel and the space charge region beneath the AlN/Si interface. We demonstrate that samples grown at lower temperature exhibit a p-doped conductive channel over a shallower depth which explains lower propagation losses in comparison with those synthesized at higher temperature. Our work highlights that this p-type channel can increase the propagation losses in the high-frequency devices but also that a memory effect associated with the previous sample growths with GaN can noticeably affect the physical properties in absence of proper reactor preparation. Hence, monitoring the acceptor dopant in-diffusion beneath the AlN/Si interface is crucial for achieving efficient GaN-on-Si microwave power devices.
Sensory satellite glial Gq-GPCR activation alleviates inflammatory pain via peripheral adenosine 1 receptor activation
Authors: Xie Alison Xiaoqiao , Madayag Aric , Minton Suzanne K. , McCarthy Ken D. , Malykhina Anna P. 
Published: August 2020

AbstractGlial fibrillary acidic protein expressing (GFAP+) glia modulate nociceptive neuronal activity in both the peripheral nervous system (PNS) and the central nervous system (CNS). Resident GFAP+ glia in dorsal root ganglia (DRG) known as satellite glial cells (SGCs) potentiate neuronal activity by releasing pro-inflammatory cytokines and neuroactive compounds. In this study, we tested the hypothesis that SGC Gq-coupled receptor (Gq-GPCR) signaling modulates pain sensitivity in vivo using Gfap-hM3Dq mice. Complete Freund’s adjuvant (CFA) was used to induce inflammatory pain, and mechanical sensitivity and thermal sensitivity were used to assess the neuromodulatory effect of glial Gq-GPCR activation in awake mice. Pharmacogenetic activation of Gq-GPCR signaling in sensory SGCs decreased heat-induced nociceptive responses and reversed inflammation-induced mechanical allodynia via peripheral adenosine A1 receptor activation. These data reveal a previously unexplored role of sensory SGCs in decreasing afferent excitability. The identified molecular mechanism underlying the analgesic role of SGCs offers new approaches for reversing peripheral nociceptive sensitization.
Impairment of spatial memory accuracy improved by Cbr1 copy number resumption and GABAB receptor-dependent enhancement of synaptic inhibition in Down syndrome model mice
Authors: Arima-Yoshida Fumiko , Raveau Matthieu , Shimohata Atsushi , Amano Kenji , Fukushima Akihiro , Watanave Masashi , Kobayashi Shizuka , Hattori Satoko , Usui Masaya , Sago Haruhiko , Mataga Nobuko , Miyakawa Tsuyoshi , Yamakawa Kazuhiro , Manabe Toshiya 
Published: August 2020

AbstractDown syndrome is a complex genetic disorder caused by the presence of three copies of the chromosome 21 in humans. The most common models, carrying extra-copies of overlapping fragments of mouse chromosome 16 that is syntenic to human chromosome 21, are Ts2Cje, Ts1Cje and Ts1Rhr mice. In electrophysiological analyses using hippocampal slices, we found that the later phase of the depolarization during tetanic stimulation, which was regulated by GABAB receptors, was significantly smaller in Ts1Cje and Ts2Cje mice than that in WT controls but not in Ts1Rhr mice. Furthermore, isolated GABAB receptor-mediated inhibitory synaptic responses were larger in Ts1Cje mice. To our knowledge, this is the first report that directly shows the enhancement of GABAB receptor-mediated synaptic currents in Ts1Cje mice. These results suggest that GABAB receptor-mediated synaptic inhibition was enhanced in Ts1Cje and Ts2Cje mice but not in Ts1Rhr mice. The Cbr1 gene, which is present in three copies in Ts1Cje and Ts2Cje but not in Ts1Rhr, encodes carbonyl reductase that may facilitate GABAB-receptor activity through a reduction of prostaglandin E2 (PGE2). Interestingly, we found that a reduction of PGE2 and an memory impairment in Ts1Cje mice were alleviated when only Cbr1 was set back to two copies (Ts1Cje;Cbr1+/+/−). However, the GABAB receptor-dependent enhancement of synaptic inhibition in Ts1Cje was unaltered in Ts1Cje;Cbr1+/+/− mice. These results indicate that Cbr1 is one of the genes responsible for DS cognitive impairments and the gene(s) other than Cbr1, which is included in Ts1Cje but not in Ts1Rhr, is responsible for the GABAB receptor-dependent over-inhibition.
Mito-oncology agent: fermented extract suppresses the Warburg effect, restores oxidative mitochondrial activity, and inhibits in vivo tumor growth
Authors: Bencze Gyula , Bencze Szilvia , Rivera Keith D. , Watson James D. , Hidvegi Mate , Orfi Laszlo , Tonks Nicholas K. , Pappin Darryl J. 
Published: August 2020

AbstractMitochondrial dysfunction and significant changes in metabolic pathways accompany cancer development and are responsible for maintaining the tumor microenvironment. Normal mitochondria can trigger intrinsic apoptosis by releasing cytochrome c into the cytosol. The survival of malignant cells highly depends on the suppression of this function. We validated that A250, a highly purified fraction of fermented wheat germ extract (FWGE), increases the carbon flux into the mitochondria, the expression of key elements of the Krebs cycle and oxidative phosphorylation (OXPHOS). The increased respiratory chain activity is related to the mitochondria’s ability to release cytochrome c into the cytosol, which triggers the apoptotic cascade. The 68% tumor growth inhibitory effect observed in the murine melanoma study is related to this effect, as proteomic analysis validated similar changes in mitochondrial protein levels in the isolated tumor tissue samples. Blood count data indicated that this effect was not accompanied by general toxicity. This study is significant, as it shows that a highly concentrated form of FWGE is an effective agent that increases normal mitochondrial functionality. The lack of hepatotoxic and general toxic effects makes A250 an excellent candidate targeting mitochondria function in cancer therapy.
Long-term effectiveness of a lifestyle intervention on the prevention of type 2 diabetes in a middle-income country
Authors: Lotfaliany Mojtaba , Mansournia Mohamad Ali , Azizi Fereidoun , Hadaegh Farzad , Zafari Neda , Ghanbarian Arash , Mirmiran Parvin , Oldenburg Brian , Khalili Davood 
Published: August 2020

AbstractThis study aims to assess the effects of a community-based lifestyle intervention program on the incidence of type 2 diabetes (T2D). For this purpose, three communities in Tehran were chosen; one community received a face-to-face educational session embedded in a long-term community-wide lifestyle intervention aimed at supporting lifestyle changes. We followed up 9,204 participants (control: 5,739, intervention: 3,465) triennially from 1999 to 2015 (Waves 1–5). After a median follow-up of 3.5 years (wave 2), the risk of T2D was 30% lower in the intervention community as compared with two control communities by (Hazard-ratio: 0.70 [95% CI 0.53; 0.91]); however, the difference was not statistically significant in the following waves. After a median follow-up of 11.9 years (wave 5), there was a non-significant 6% reduction in the incidence of T2D in the intervention group as compared to the control group (Hazard-ratio: 0.94 [0.81, 1.08]). Moreover, after 11.9 years of follow-up, the intervention significantly improved the diet quality measured by the Dietary Approaches to Stop Hypertension concordance (DASH) score. Mean difference in DASH score in the intervention group versus control group was 0.2 [95% CI 0.1; 0.3]. In conclusion, the intervention prevented T2D by 30% in the short-term (3.5 years) but not long-term; however, effects on improvement of the diet maintained in the long-term.Registration: This study is registered at IRCT, a WHO primary registry (https://irct.ir). The registration date 39 is 2008-10-29 and the IRCT registration number is IRCT138705301058N1.
From waste to health: sustainable exploitation of grape pomace seed extract to manufacture antioxidant, regenerative and prebiotic nanovesicles within circular economy
Authors: Manca Maria Letizia , Casula Eleonora , Marongiu Francesca , Bacchetta Gianluigi , Sarais Giorgia , Zaru Marco , Escribano-Ferrer Elvira , Peris José Esteban , Usach Iris , Fais Sara , Scano Alessandra , Orrù Germano , Maroun Richard G. , Fadda Anna Maria , Manconi Maria 
Published: August 2020

AbstractPomace seed extract loaded vesicles were prepared as promising technological and green solution to exploit agri-food wastes and by-products, and develop high value-added products for human health. An antioxidant extract rich in bioactive compounds (epicatechins, catechin, gallic acid, quercetin and procynidins) was obtained from the seeds isolated from the pomace of Cannonau red grape cultivar. The extract was incorporated into phospholipid vesicles ad hoc formulated for intestinal delivery, by combining them, for the first time, whit a maltodextrin (Glucidex). Glucidex-transfersomes, glucidex-hyalurosomes and glucidex-hyalutransferomes were prepared, characterized and tested. Glucidex-liposomes were used as reference. All vesicles were small in size (~ 150 nm), homogeneously dispersed and negatively charged. Glucidex-transfersomes and especially glucidex-hyalutransfersomes disclosed an unexpected resistance to acidic pH and high ionic strength, as they maintained their physico-chemical properties (size and size distribution) after dilution at pH 1.2 simulating the harsh gastric conditions. Vesicles were highly biocompatible and able to counteract the oxidative damages induced in Caco-2 cells by using hydrogen peroxide. Moreover, they promoted the formation ofLactobacillus reuteribiofilm acting as prebiotic formulation. Overall results suggest the potential of glucidex-hyalutransfersomes as food supplements for the treatment of intestinal disorders.
Plasma cell-free DNA methylation marks for episodic memory impairment: a pilot twin study
Authors: Konki M. , Lindgren N. , Kyläniemi M. , Venho R. , Laajala E. , Ghimire B. , Lahesmaa R. , Kaprio J. , Rinne J. O. , Lund R. J. 
Published: August 2020

AbstractDecline in episodic memory performance usually causes the first clinical symptoms of Alzheimer’s disease. At present, Alzheimer’s disease can only be diagnosed at a very late stage when neurodegeneration and cognitive impairment is already irreversible. New early disease markers are needed for earlier and more efficient Alzheimer’s disease intervention. To identify early disease markers, we implemented a genome-wide bisulphite sequencing method for the analysis of plasma cell-free DNA methylation profiles and compared differences associated with episodic memory performance in Finnish twin pairs. A noticeable amount of cell-free DNA was present in plasma, however, the amounts as well as the genomic coverage of these fragments varied substantially between individuals. We found no significant markers associated with episodic memory performance in the twins’ plasma cell-free DNA methylation profiles. Furthermore, our results indicate that due to the low genomic coverage of cell-free DNA fragments and the variety in these fragments between individuals, the implemented genome-wide bisulphite sequencing method is not optimal for comparing cell-free DNA methylation differences between large groups of individuals.
Dust and star formation in the centre of NGC 3311
Authors: Richtler T. , Hilker M. , Arnaboldi M. , Barbosa C. E. 
Published: August 2020

Context. NGC 3311 is the central galaxy of the Hydra I galaxy cluster. It has a hot interstellar medium and hosts a central dust lane with emission lines. These dust lanes are frequent in elliptical galaxies, but the case of NGC 3311 might be particularly interesting for problems of dust lifetime and the role of cool gas in the central parts.
Aims. We aim to use archival HST images and MUSE data to investigate the central dust structure of NGC 3311.
Methods. We used the tool PyParadise to model the stellar population and extract the emission lines.
Results. The HST/ACS colour map reveals the known dust structures, but also blue spots, which are places of strong line emission. A dusty “mini-jet” emanates from the centre. The distribution of the emission line gas matches the dust silhouette almost exactly. Close to the brightest Hα emission, the ratio [NII]/Hα resembles that of HII-regions; in the outer parts, [NII] gets stronger and is similar to LINERLow-ionization nuclear emission-line region -like spectra. The gas kinematics is consistent with that of a rotating disc. The Doppler shifts of the strongest line emissions, which indicate the areas of highest star formation activity, smoothly fit into the disc symmetry. The metallicity is supersolar. The presence of neutral gas is indicated by the fit residuals of the stellar NaI D absorption line, which we interpret as interstellar absorption. We estimate the mass of the neutral gas to be of the order of the X-ray mass. The dynamical mass infers a stellar population of intermediate age, whose globular clusters have already been identified.
Conclusions. Our findings can be harmonised in a scenario in which the star formation is triggered by the accretion of cold gas onto a pre-existing gas/dust disc or ring. Newly produced dust then contributes to the longevity of the dust.
Chandra reveals a luminous Compton-thick QSO powering a Lyα blob in a z = 4 starbursting protocluster
Authors: Vito F. , Brandt W. N. , Lehmer B. D. , Vignali C. , Zou F. , Bauer F. E. , Bremer M. , Gilli R. , Ivison R. J. , Spingola C. 
Published: August 2020

Context. Galaxy clusters in the local universe descend from high-redshift overdense regions known as protoclusters. The large gas reservoirs and high rate of galaxy interaction in protoclusters are expected to enhance star-formation activity and trigger luminous supermassive black-hole accretion in the nuclear regions of the host galaxies.
Aims. We investigated the active galactic nucleus (AGN) content of a gas-rich and starbursting protocluster at z = 4.002, known as the Distant Red Core (DRC). In particular, we search for luminous and possibly obscured AGN in 13 identified members of the structure, and compare the results with protoclusters at lower redshifts. We also test whether a hidden AGN can power the Lyα blob (LAB) detected with VLT/MUSE in the DRC.
Methods. We observed all of the identified members of the structure with 139 ks of Chandra ACIS-S imaging. Being less affected by absorption than optical and IR bands, even in the presence of large column densities of obscuring material, X-ray observations are the best tools to detect ongoing nuclear activity in the DRC galaxies.
Results. We detect obscured X-ray emission from the two most gas-rich members of the DRC, named DRC-1 and DRC-2. Both of them are resolved into multiple interacting clumps in high-resolution Atacama Large Millimeter Array and Hubble Space Telescope observations. In particular, DRC-2 is found to host a luminous (L2−10 keV ≈ 3 × 1045 erg s−1 ) Compton-thick (NH ≳ 1024 cm−2) quasar (QSO) candidate, comparable to the most luminous QSOs known at all cosmic times. The AGN fraction among DRC members is consistent with results found for lower redshift protoclusters. However, X-ray stacking analysis reveals that supermassive black hole (SMBH) accretion is likely also taking place in other DRC galaxies that are not detected individually by Chandra.
Conclusions. The luminous AGN detected in the most gas-rich galaxies in the DRC and the widespread SMBH accretion in the other members, which is suggested by stacking analysis, point toward the presence of a strong link between large gas reservoirs, galaxy interactions, and luminous and obscured nuclear activity in protocluster members. The powerful and obscured QSO detected in DRC-2 is likely powering the nearby LAB detected with VLT/MUSE, possibly through photoionization; however, we propose that the diffuse Lyα emission may be due to gas shocked by a massive outflow launched by DRC-2 over a ≈10 kpc scale.