bluuebunny/crossref_metadata_2025_split huggingface dataset:
Three-dimensional structure analysis of melanocytes and keratinocytes in senile lentigo
Authors: Mizutani Yuki , Yamashita Mika , Hashimoto Rie , Atsugi Toru , Ryu Akemi , Hayashi Akinobu , Rikimaru-Nishi Yukiko , Ohta Keisuke 
Published: September 2020

AbstractSenile lentigo or age spots are hyperpigmented macules of skin that commonly develop following long-term exposure to ultraviolet radiation. This condition is caused by accumulation of large numbers of melanosomes (melanin granules) produced by melanocytes within neighboring keratinocytes. However, there is still no consensus regarding the melanosome transfer mechanism in senile lentigo. To date, most pathohistological studies of skin have been two-dimensional and do not provide detailed data on the complex interactions of the melanocyte–keratinocyte network involved in melanosome transfer.We performed a three-dimensional reconstruction of the epidermal microstructure in senile lentigo using three different microscopic modalities to visualize the topological melanocyte–keratinocyte relationship and melanosome distribution. Confocal laser microscopy images showed that melanocyte dendritic processes are more frequently branched and elongated in senile lentigo skin than in normal skin. Serial transmission electron micrographs showed that dendritic processes extend into intercellular spaces between keratinocytes. Focused ion beam-scanning electron micrographs showed that dendritic processes in senile lentigo encircle adjacent keratinocytes and accumulate large numbers of melanosomes. Moreover, melanosomes transferred to keratinocytes are present not only in the supranuclear area but throughout the perinuclear area except on the basal side.The use of these different microscopic methods helped to elucidate the three-dimensional morphology and topology of melanocytes and keratinocytes in senile lentigo. We show that the localization of melanosomes in dendritic processes to the region encircling recipient keratinocytes contributes to efficient melanosome transfer in senile lentigo.
A unique case of medulla oblongata epidermoid cyst
Authors: Tuan Tran Anh , Duc Nguyen Minh 
Published: September 2020

AbstractEpidermoid cysts (ECs) are slow-growing, benign tumors that represent &lt;2% of all intracranial tumors. ECs can be divided into following two types: extra-axial and intra-axial. Extra-axial ECs are most often positioned in the cerebellopontine angle. Intra-axial ECs, which are also referred to as intraparenchymal ECs, are most commonly found in supratentorial structures, such as the frontal and temporal lobes, accounting for &lt;2% of all intracranial ECs and are especially rare in children. In this report, we described a unique case of medulla oblongata EC in a child, to contribute this knowledge to the existing body of literature.
Ankylosis of the temporomandibular joint—impression free CAD/CAM based joint replacement using patient-specific implants
Authors: Thiem D G E , Al-Nawas B , Kämmerer P W 
Published: September 2020

Abstract
In recent years, alloplastic temporomandibular joint (TMJ) replacement has become a permissible procedure for the reconstruction of severely destroyed TMJs. The use of computer-aided design/computer-aided manufacturing (CAD/CAM) has extended the range of applications to complex anatomical situations. The aim of the treatment is to improve the usually restricted mouth opening and thus oral hygiene and nutrition, which leads to a regular improvement in the general quality of life. The following case report describes the bilateral replacement of ankylotically destroyed TMJs using patient-specific endoprostheses with simultaneous displacement of the maxilla. Innovative in the case described is the impression-free CAD/CAM planning, whereby the upper and lower prostheses were produced on the basis of 3D printed patient models.
TIC 278825952: a triply eclipsing hierarchical triple system with the most intrinsically circular outer orbit
Authors: Mitnyan T , Borkovits T , Rappaport S A , Pál A , Maxted P F L 
Published: September 2020

ABSTRACT
We report the discovery of a compact triply eclipsing triple star system in the southern continuous viewing zone of the TESS space telescope. TIC 278825952 is a previously known, but unstudied circular eclipsing binary with a period of 4.781 d with a tertiary component in a wider, circular orbit of 235.55-d period that was found from three sets of third-body eclipses and from light travel-time effect dominated eclipse timing variations. We performed a joint photodynamical analysis of the eclipse timing variation curves, photometric data, and the spectral energy distribution, coupled with the use of PARSEC stellar isochrones. We find that the inner binary consists of slightly evolved, near twin stars of masses of 1.12 and 1.09 M⊙ and radii of 1.40 and 1.31 R⊙. The third, less massive star has a mass of 0.75 M⊙ and radius of 0.70 R⊙. The low mutual inclination and eccentricities of the orbits show that the system is highly coplanar and surprisingly circular.
Experimental glioma with high bHLH expression harbor increased replicative stress and are sensitive toward ATR inhibition
Authors: Koch Marilin Sophia , Czemmel Stefan , Lennartz Felix , Beyeler Sarah , Rajaraman Srinath , Przystal Justyna Magdalena , Govindarajan Parameswari , Canjuga Denis , Neumann Manfred , Rizzu Patrizia , Zwirner Stefan , Hoetker Michael Stefan , Zender Lars , Walter Bianca , Tatagiba Marcos , Raineteau Olivier , Heutink Peter , Nahnsen Sven , Tabatabai Ghazaleh 
Published: September 2020

Abstract

Background
The overexpression of (basic)helix-loop-helix ((b)HLH) transcription factors (TFs) is frequent in malignant glioma. We investigated molecular effects upon disruption of the (b)HLH network by a dominant-negative variant of the E47 protein (dnE47). Our goal was to identify novel molecular subgroup-specific therapeutic strategies.

Methods
Glioma cell lines LN229, LNZ308, and GS-2/GS-9 were lentivirally transduced. Functional characterization included immunocytochemistry, immunoblots, cytotoxic, and clonogenic survival assays in vitro, and latency until neurological symptoms in vivo. Results of cap analysis gene expression and RNA-sequencing were further validated by immunoblot, flow cytometry, and functional assays in vitro.

Results
The induction of dnE47-RFP led to cytoplasmic sequestration of (b)HLH TFs and antiglioma activity in vitro and in vivo. Downstream molecular events, ie, alterations in transcription start site usage and in the transcriptome revealed enrichment of cancer-relevant pathways, particularly of the DNA damage response (DDR) pathway. Pharmacologic validation of this result using ataxia telangiectasia and Rad3 related (ATR) inhibition led to a significantly enhanced early and late apoptotic effect compared with temozolomide alone.

Conclusions
Gliomas overexpressing (b)HLH TFs are sensitive toward inhibition of the ATR kinase. The combination of ATR inhibition plus temozolomide or radiation therapy in this molecular subgroup are warranted.
Spatial shifts in size structure, phylogenetic diversity, community composition and abundance of small eukaryotic plankton in a coastal upwelling area of the northern South China Sea
Authors: Gong Fahui , Li Guihao , Wang Yaping , Liu Qinyu , Huang Fangjuan , Yin Kedong , Gong Jun 
Published: September 2020

Abstract
Summer upwelling off Hainan Island (northern South China Sea) is influenced by fresher coastal water masses. To assess the ecological effects of this complicated oceanography, a cruise was conducted in August 2016. We investigated the spatial patterns of phytoplankton size structure, diversity and quantity of small eukaryotes (0.2–20 μm) across the upwelling system. In the inner-shelf waters, nanophytoplankton contributed a higher proportion to phytoplankton biomass than microphytoplankton and picophytoplankton. High-throughput sequencing of 18S rRNA genes and community analysis indicated that the horizontal variation in community structure of small eukaryotes was larger than the vertical. The relative sequence proportions of dinoflagellates, diatoms, ciliates and Micromonas in the surface layer gradually decreased from the inner shelf to the slope, whereas the Rhizaria, Bathycoccus and Ostreococcus exhibited an opposite trend. The spatial patterns of taxon-specific rDNA copy numbers were similar to the patterns based on the relative proportions. Redundancy analysis revealed that turbidity, salinity and ${\mathrm{NO}}_3^{-}$ were the most important environmental variables in structuring the community of small eukaryotes. The cell abundance of photosynthetic picoeukaryotes was significantly correlated with the rDNA abundance of Mamiellophyceae and the cell abundance of nanoeukaryotes was significantly correlated with the rDNA abundance of dinoflagellates plus diatoms.
Earnings Expectations during the COVID-19 Crisis*
Authors: Landier Augustin , Thesmar David 
Published: September 2020

Abstract
We analyze the dynamics of earnings forecasts and discount rates implicit in valuations during the COVID-19 crisis. Forecasts over 2020 earnings have been progressively reduced by 16%. Longer-run forecasts have reacted much less. We estimate an implicit discount rate going from 8.5% in mid-February to 11% at the end of March and reverting to its initial level in mid-May. Over the period, the unlevered asset risk premium increases by 50bp, the leverage effect also increases by 50bp, while the risk free rate decreases by 100bp. Hence, analysts’ forecast revisions explain all of the decrease in equity values between January 2020 and mid-May 2020.
Authors have furnished an Internet Appendix, which is available on the Oxford University Press Web site next to the link to the final published paper online.
Hysteresis in Price Efficiency and the Economics of Slow-Moving Capital
Authors: Dow James , Han Jungsuk , Sangiorgi Francesco 
Published: September 2020

Abstract
Will arbitrage capital flow into markets experiencing shocks, mitigating adverse effects on price efficiency? Not necessarily. In a dynamic model with privately informed capital-constrained arbitrageurs, price efficiency plays a dual role, determining both the profitability of new arbitrage and the ability to close existing positions profitably. An adverse shock to efficiency lengthens arbitrage duration, effectively reducing the amount of arbitrage capital available for new positions. If this falls below a critical mass, arbitrage capital flows out, amplifying the impact on price efficiency. This creates endogenous regimes: temporary shocks can trigger “hysteresis,” a persistent shift in price efficiency.
Moral Religiosities: How Morality Structures Religious Understandings during the Transition to Adulthood
Authors: Rotolo Michael 
Published: September 2020

Abstract
Religiosity remains an important sociological concept, from assessing religion’s effects on various outcomes to describing large-scale religious change. And yet conceptualizing religiosity—as a measure of intensity of religious practice—requires accounting for how respondents understand religious practice. Drawing on four waves of longitudinal interview data from the National Study of Youth and Religion (NSYR), this paper examines the religious understandings of young Americans as they develop over 10 years. I find that respondents’ religious understandings are shaped by deeper moral orientations that broadly structure their lives. From these moral orientations, I theorize four ideal types of religious practitioners that help explain complex patterns of religiosity in America—the Congregant, the Believer, the Spiritualist, and the Metaphysician. Recognizing the moral orders that structure young Americans’ religious understandings opens new pathways for theorizing religion’s influence and change over time.
Cow efficiency: modeling the biological and economic output of a Michigan beef herd
Authors: Thompson Logan R , Beck Matthew R , Buskirk Daniel D , Rowntree Jason E , McKendree Melissa G S 
Published: September 2020

Abstract
In recent decades, beef cattle producers have selected cattle for biological traits (i.e., improved growth) to maximize revenue, leading to an increase in average cow body size. However, matching cow size to the production environment would allow producers to maximize productivity and economic returns per unit of land. This may help meet the goals of sustainable intensification, but environmental complexity and varying cow-calf production systems dictates a regional approach. The objective of this experiment was to examine the biological efficiency and economic returns of a Northern Michigan cow-calf system. We hypothesized that biological efficiency and economic returns would decrease with increasing cow body size. Data were collected from a Red Angus cow herd located at the Lake City AgBio Research Center in Lake City, MI from 2011 to 2018 on cow age, weight, and body condition score at weaning, and subsequent 205 d adjusted calf weaning weight (WW), sex, and yearling weight. Biological efficiency was defined as WW as a percentage of cow body weight (DBW). Enterprise budgeting techniques were used to calculate expected net returns from 2011 to 2018 after classifying cows into 11 BW tiers at 22.67 kg intervals beginning at 430.83 kg. Forward-looking net present value (NPV) was calculated using the same tier system, for a 10-yr production cycle with the baseline being a 200 d grazing season. Weaning weight increased with increasing DBW (P &lt; 0.01), but the percentage of cow body weight weaned was reduced by −38.58 × Ln(DBW) (P &lt; 0.01). This led to cows weaning 26.38 kg/ha more with every 100 kg drop in DBW. Expected net returns from 2011 to 2018 did not differ by DBW tier on a per cow basis but did on a per ha basis with a decrease in $10.27/ha with each increase in DBW tier (P &lt; 0.01). Net present value was maximized in the baseline scenario at 453.51 kg DBW and decreased in value as DBW increased. These results suggest that for a Northern Midwestern cow-calf herd, comparatively lighter cows provide a higher economic value on a land basis.
Effects of cutting time and maceration on preference and nitrogen balance in beef steers fed mixed birdsfoot trefoil–timothy grass hay cut at sunrise or sundown
Authors: Silva Luiz H P , Brito André F , Lafrenière Carole , Berthiaume Robert 
Published: September 2020

Abstract
Forages cut at sundown usually contain a greater concentration of nonstructural carbohydrates (NSC) than those cut at sunrise. Maceration can speed up the rate of moisture loss of cut forage during field drying and reduce NSC utilization by plant cells. We aimed to evaluate the effects of cutting time and forage maceration on feed preference, apparent total tract digestibility of nutrients, and N balance in growing steers. A mixed sward of birdsfoot trefoil and timothy grass was divided into two halves, with the first half cut at sundown (1800 h) after a sunny day and the second half at sunrise (0600 h) the next day. Approximately 50% of the sundown- and sunrise-cut herbage were macerated. Forages were harvested as hay resulting in four treatments: 1) sunrise-cut hay (AM); 2) AM plus maceration (AM-M); 3) sundown-cut hay (PM); and 4) PM plus maceration (PM-M). Hays were offered as the sole feed source to four crossbred steers (296.1 ± 7.25 kg) according to a 4 × 4 Latin square design with a 2 × 2 factorial arrangement of treatments. Each period lasted 21 d with 14 d for diet adaptation and 7 d for collection. Hays cut at sundown had 15% greater NSC than those cut at sunrise. A cutting time by maceration interaction was found (P &lt; 0.05) for intake and apparent digestibility of crude protein (CP), indicating that these two variables decreased more when maceration was applied to sundown- versus sunrise-cut hays. Similarly, interaction effects were observed (P &lt; 0.05) for total digestible nutrients and digestible energy, showing that maceration decreased the energetic value of sundown-cut hays but did not change that of sunrise-cut hays. Steers fed hays cut at sundown had decreased urinary N excretion and improved retained N (P &lt; 0.05), whereas N retention was reduced by maceration (P &lt; 0.05). In addition, six crossbred steers were used to assess feed preference, 2 wk before (period 1) and 1 wk after (period 2) the digestibility trial. Animals were randomly assigned to receive a sequence of the four hays combined in pairs. The intake rate was greater for sundown- than sunrise-cut hays, and it was decreased by maceration. Steers showed the greatest preference for PM hay, while AM-M was the most rejected. In conclusion, shifting forage cutting from sunrise to sundown increased hay NSC concentration, which resulted in improved N utilization and preference. Forage maceration during field drying decreased CP concentration and N retention in beef steers under the conditions of our study.
Extrusion of soybean hulls does not increase digestibility of amino acids or concentrations of digestible and metabolizable energy when fed to growing pigs
Authors: Rodriguez Diego A , Lee Su A , de Godoy María R C , Stein Hans H 
Published: September 2020

AbstractTwo experiments were conducted to determine effects of extrusion on energy and nutrient digestibility in soybean hulls. One source of soybean hulls was ground and divided into two batches. One batch was used without further processing, whereas the other batch was extruded. In Exp. 1, four diets were formulated to determine crude protein (CP) and amino acid (AA) digestibility in soybean hulls. A soybean meal-based diet in which soybean meal provided all the CP and AA was formulated. Two diets were formulated to contain 30% nonextruded or extruded soybean hulls and 18% soybean meal. An N-free diet that was used to determine the endogenous losses of CP and AA was also used. Eight growing barrows (initial body weight = 37.0 ± 3.9 kg) had a T-cannula installed in the distal ileum and were allotted to a replicated 4 × 4 Latin square design. Each experimental period lasted 7 d with the initial 5 d being the adaptation period and ileal digesta were collected for 8 h on day 6 and 7. Results indicated that extrusion of soybean hulls did not change the standardized ileal digestibility (SID) of CP and most AA with the exception that the SID of Ile and Leu tended (P &lt; 0.10) to be greater in extruded than nonextruded soybean hulls. In Exp. 2, three diets were formulated to determine energy digestibility in soybean hulls. One corn-soybean meal based basal diet, and two diets that contained corn, soybean meal, and 32% extruded or nonextruded soybean hulls were formulated. Twenty-four growing barrows (initial body weight = 59.9 ± 3.4 kg) were allotted to a randomized complete block design. Pigs were housed individually in metabolism crates and feces and urine were collected separately for 4 d after 5 d of adaptation. The apparent total tract digestibility (ATTD) of gross energy (GE) and the digestible energy (DE) and metabolizable energy (ME) were reduced (P &lt; 0.05) in diets containing nonextruded or extruded soybean hulls compared with the basal diet. However, the ATTD of GE and values for DE and ME in soybean hulls were not improved by extrusion. Likewise, extrusion did not change the concentration of total dietary fiber in soybean hulls. In conclusion, there were no effects of extrusion of soybean hulls on SID of AA, energy digestibility, or ME concentration in soybean hulls.
Effects of chlorpyrifos on primary gill cell culture of Lake Van fish (Alburnus tarichiGüldenstaadt 1814)
Authors: Oğuz Ahmet Regaib , Kaval Oğuz Elif , Özok Necati 
Published: September 2020

AbstractLake Van fish (Alburnus tarichi Guldenstadt 1814) is the only fish that can adapt to the extreme conditions (pH 9.8 salinity 0.2% and alkalinity 151.2 meq/L) of Lake Van. In this study, it was aimed to determine the cytotoxic and genotoxic effects of chlorpyrifos (CPF) on Lake Van fish primary gill cell culture. Gill epithelium from Lake Van fish was isolated enzymatically and grown in primary culture on Leibovitz’s L-15 medium. After different doses (0.01, 0.1, 1, and 10 μM) of CPF were applied to the gill cells, the total oxidant status (TOS), total antioxidant status (TAS), malondialdehyde (MDA), and DNA damage levels (8-hydroxyguanine (8-OHdG)) were examined at the end of 24 and 48 h. It was determined that the TOS, MDA, and 8-OHdG levels increased in the cells exposed to high doses (1 and 10 μM) of CPF and the TAS was decreased (P &lt; 0.05). It was revealed from this study that CPF administered at a dose higher than 1 μM can cause oxidative stress and DNA damage in the primary gill cell culture of Lake Van fish. In addition, the findings showed that Lake Van fish primary gill cell culture was useful in determining the effects of toxic substances likely to be the contaminants of a lake.
Deltamethrin Exposure Inhibits Adult Hippocampal Neurogenesis and Causes Deficits in Learning and Memory in Mice
Authors: Hossain Muhammad M , Belkadi Abdelmadjid , Al-Haddad Sara , Richardson Jason R 
Published: September 2020

Abstract
Deficits in learning and memory are often associated with disruption of hippocampal neurogenesis, which is regulated by numerous processes, including precursor cell proliferation, survival, migration, and differentiation to mature neurons. Recent studies demonstrate that adult born neurons in the dentate gyrus (DG) in the hippocampus can functionally integrate into the existing neuronal circuitry and contribute to hippocampal-dependent learning and memory. Here, we demonstrate that relatively short-term deltamethrin exposure (3 mg/kg every 3 days for 1 month) inhibits adult hippocampal neurogenesis and causes deficits in learning and memory in mice. Hippocampal-dependent cognitive functions were evaluated using 2 independent hippocampal-dependent behavioral tests, the novel object recognition task and Morris water maze. We found that deltamethrin-treated mice exhibited profound deficits in novel object recognition and learning and memory in water maze. Deltamethrin exposure significantly decreased bromodeoxyuridine (BrdU)-positive cells (39%) and Ki67+ cells (47%) in the DG of the hippocampus, indicating decreased cellular proliferation. In addition, deltamethrin-treated mice exhibited a 44% decrease in nestin-expressing neural progenitor cells and a 38% reduction in the expression of doublecortin (DCX), an early neuronal differentiation marker. Furthermore, deltamethrin-exposed mice exhibited a 25% reduction in total number of granule cells in the DG. These findings indicate that relatively short-term exposure to deltamethrin causes significant deficits in hippocampal neurogenesis that is associated with impaired learning and memory.