bluuebunny/crossref_metadata_2025_split huggingface dataset:
MiR‐26b suppresses hepatocellular carcinoma development by negatively regulating ZNRD1 and Wnt/β‐catenin signaling
Authors: Hu Xiaobo , Wang Ruifang , Ren Zhigang , Liu Xiaorui , Gu Junsheng , Cui Guangying , Li Qinggang 
Published: October 2019

AbstractPrevious studies have indicated that Zinc ribbon domain‐containing 1 (ZNRD1) is attributed to the carcinogenesis of human tumors. However, the role of ZNRD1 and its regulation in hepatocellular carcinoma (HCC) are still largely unclear. In this study, we examined the expression profiles of ZNRD1 in HCC tissues by immunohistochemistry (IHC) and publicly datasets analysis. In vitro and in vivo experiments were conducted to identify the function of ZNRD1 in HCC. In addition, miRNA potentially targeting ZNRD1 was predicted by bioinformatics analysis and further verified via in vitro experiments. Our results revealed that ZNRD1 was frequently upregulated in HCC tissues compared with that in nontumor tissues. High ZNRD1 expression in HCC tissues was positively associated with advanced tumor stage and poor prognosis. Function experiments showed that knockdown of ZNRD1 inhibited cell growth and invasion in vitro, and suppressed tumor development in vivo. Moreover, ZNRD1 promoted the activation of Wnt/β‐catenin signaling pathway in HCC. Importantly, miR‐26b directly inhibited the transcription activity of ZNRD1. Overexpression of ZNRD1 dramatically abolished the inhibitory effects of miR‐26b on HCC cells. Taken together, our results uncover a novel mechanistic role for miR‐26b/ZNRD1 axis in HCC, proposing ZNRD1 inhibition as a potent therapeutic strategy for hepatocellular carcinoma.
Nck adapter proteins promote podosome biogenesis facilitating extracellular matrix degradation and cancer invasion
Authors: Chaki Sankar P. , Barhoumi Rola , Rivera Gonzalo M. 
Published: October 2019

AbstractBackgroundPodosomes are membrane‐bound adhesive structures formed by actin remodeling. They are capable of extracellular matrix (ECM) degradation, which is a prerequisite for cancer cell invasion and metastasis. The signaling mechanism of podosome formation is still unknown in cancer. We previously reported that Nck adaptors regulate directional cell migration and endothelial lumen formation by actin remodeling, while deficiency of Nck reduces cancer metastasis. This study evaluated the role of Nck adaptors in podosome biogenesis and cancer invasion.MethodsThis study was conducted in vitro using both healthy cells (Human Umbilical Vein Endothelial Cell, 3T3 fibroblasts) and cancer cells (prostate cancer cell line; PC3, breast cancer cell line; MDA‐MB‐231). Confocal and TIRF imaging of cells expressing Green Fluorescence Protein (GFP) mutant under altered levels of Nck or downstream of kinase 1 (Dok1) was used to evaluate the podosome formation and fluorescent gelatin matrix degradation. Levels of Nck in human breast carcinoma tissue sections were detected by immune histochemistry using Nck polyclonal antibody. Biochemical interaction of Nck/Dok1 was detected in podosome forming cells using immune precipitation and far‐western blotting.ResultsThis study demonstrates that ectopic expression of Nck1 and Nck2 can induce the endothelial podosome formation in vitro. Nck silencing by short‐hairpin RNA blocked podosome biogenesis and ECM degradation in cSrc‐Y530F transformed endothelial cells in this study. Immunohistochemical analysis revealed the Nck overexpression in human breast carcinoma tissue sections. Immunoprecipitation and far‐western blotting revealed the biochemical interaction of Nck/p62Dok in podosome forming cells.ConclusionsNck adaptors in interaction with Dok1 induce podosome biogenesis and ECM degradation facilitating cancer cell invasion, and therefore a bona fide target of cancer therapy.
HPLC‐DAD Analysis, Antioxidant and Protective Effects of Tunisian Rhanterium suaveolens against Acetamiprid Induced Oxidative Stress on Mice Erythrocytes
Authors: Chelly Sabrine , Chelly Meryam , Salah Hichem Ben , Athmouni Khaled , Bitto Alessandra , Sellami Hanen , Kallel Choumous , Bouaziz‐Ketata Hanen 
Published: October 2019

AbstractThe present study was performed to assess the HPLC‐DAD analysis as well as antioxidant and protective effects of Tunisian Rhanterium suaveolens (Rs) against acetamiprid (ACT) induced oxidative stress on mice erythrocytes. The in vitro assays showed that the methanolic extract of Rs has an impressive antioxidant effect proved by testing the total antioxidant and scavenging activities using BCB, DPPH and ABTS assays, respectively. Moreover, qualitative and quantitative analysis using HPLC‐DAD revealed the richness of Rs in polyphenols where p‐Coumaric, Apigenin‐7‐glucoside and Ferulic acid were detected as the most abundant polyphenols. In the in vivo experiment, ACT, used as a toxicity model, was given to mice at a dose of 20 mg/kg. The latter was the origin of hemolytic anemia characterized by a significant decrease in red blood cells, hemoglobin and hematocrit levels and an increase in bilirubin, LDH, osmotic fragility, reticulocytes and white blood cells number. Characteristic erythrocyte morphological alterations were also determined as spherocytosis, schistocytosis and dacryocystitis. The oxidative status of ACT‐treated mice was also altered manifested by a significant increase in MDA and GSH levels and a decrease in SOD, CAT and GPx activities. When receiving the Rs methanolic extract at a dose of 300 mg/kg, all the parameters cited above were restored in mice. These remarkable corrections could only confirm the important antioxidant effect and the noticeable protective properties that possess Rs owing to its broad range of secondary bioactive metabolites.
Identification of patients at higher risk for myocardial injury following elective coronary artery intervention
Authors: Abu Sharar Haitham , Helfert Stefanie , Vafaie Mehrshad , Pleger Sven T. , Chorianopoulos Emmanuel , Bekeredjian Raffi , Katus Hugo A. , Giannitsis Evangelos 
Published: October 2019

AbstractObjectivesTo evaluate myocardial injury and infarction (MI) following elective percutaneous coronary intervention (PCI).BackgroundThe substantially higher analytical power of high‐sensitivity troponin (hsTn) assays allows detection of minor cardiac troponin (cTn) levels, which may be useful in monitoring myocardial injury and guiding therapies.MethodsSerial hsTnT measurements were conducted in patients undergoing elective PCI and were related to the extent of coronary artery disease (CAD) as reflected by the SYNTAX score risk categories and American College of Cardiology/American Heart Association classification of coronary lesions. Myocardial injury and MI were diagnosed according to the second and third versions of universal MI definition.ResultsThe study population consisted of 530 patients, who were grouped into low (41.3%), intermediate (35.4%), and high (23.3%) SYNTAX risk categories. The treated coronary lesions were classified into A 7.8%, B1 24.1%, B2 21.1%, C1 24.6%, and C2 22.4%. Postprocedural hsTnT increases correlated significantly with the complexity of treated coronary lesions (p < .05) and CAD magnitude (p < .05). Rates of MI type 4a according to the second and third MI definition criteria were 98 (27.5%) and 15 (4.2%) cases in patients with normal baseline hsTnT values (N = 357, 67.4%), as well as 137 (79.2%) and 27 (15.6%) cases in those with elevated baseline hsTnT values (N = 173, 32.6%), respectively.ConclusionsAfter elective PCI, cTn releases correlate significantly with lesion complexity and CAD extent. Use of hsTnT assay enables precise monitoring of PCI‐related myocardial injury and may identify patients at higher risk for ischemic events, who may benefit from potent platelet inhibition, which needs to be investigated in randomized trials.
Vascular complications associated with percutaneous left ventricular assist device placement: A 10‐year US perspective
Authors: Patel Nilay , Sharma Akshit , Dalia Tarun , Rali Aniket , Earnest Matthew , Tadros Peter , Wiley Mark , Hockstad Eric , Mehta Ashwani , Thors Axel , Hance Kirk , Gupta Kamal 
Published: October 2019

AbstractBackgroundOver the last decade, there has been a significant increase in the use of percutaneous left ventricular assist devices(p‐LVADs). p‐LVADs are being increasingly used during complex coronary interventions and for acute cardiogenic shock. These large bore percutaneous devices have a higher risk of vascular complications. We examined the vascular complication rates from the use of p‐LVAD in a national database.MethodsWe conducted a secondary analysis of the National In‐patient Sample (NIS) dataset from 2005 till 2015. We used the ICD‐9‐CM procedure codes 37.68 and 37.62 for p‐LVAD placement regardless of indications. We investigated common vascular complications, defining them by the validated ICD 9 CM codes. χ2 test and t test were used for categorical and continuous variables, respectively for comparison.ResultsA total of 31,263 p‐LVAD placements were identified during the period studied. A majority of patients were male (72.68%) and 64.44% were white. The overall incidence of vascular complications was 13.53%, out of which 56% required surgical treatment. Acute limb thromboembolism and bleeding requiring transfusion accounted for 27.6% and 21.8% of all vascular complications. Occurrence of a vascular complication was associated with significantly higher in‐hospital mortality (37.77% vs. 29.95%, p < .001), length of stay (22.7 vs. 12.2 days, p < .001) and cost of hospitalization ($ 161,923 vs. $ 95,547, p < .001).ConclusionsThere is a high incidence of vascular complications with p‐LVAD placement including need for vascular surgery. These complications are associated with a higher in‐hospital, LOS and hospitalization costs. These findings should be factored into the decision‐making for p‐LVAD placement.
Severe myocarditis due to influenza A(H1N1)pdm09 viral infection in a young woman successfully treated with intravenous zanamivir: A case report
Authors: Mazzitelli Maria , Garofalo Eugenio , Bruni Andrea , Barreca Giorgio Settimo , Quirino Angela , Giancotti Aida , Serapide Francesca , Indolfi Ciro , Matera Giovanni , Navalesi Paolo , Trecarichi Enrico Maria , Torti Carlo , Longhini Federico ,   The IMAGES (Integrated MAnaGEment of Sepsis) Group
Published: October 2019

AbstractIn patients with influenza‐related myocarditis, prompt diagnosis and treatment are important. Intravenous zanamivir can be an alternative to oral oseltamivir, especially in severe cases and when drug intestinal malabsorption is suspected or proven.
Crystal Engineering with Multipoint Halogen Bonding: Double Two‐Point Donors and Acceptors at Work
Authors: Bulfield David , Engelage Elric , Mancheski Lucas , Stoesser Julian , Huber Stefan M. 
Published: October 2019

AbstractThe combination of singly or doubly bidentate halogen‐bond donors with double bidentate acceptors was investigated as a supramolecular synthon in crystal engineering. The crystal topologies obtained feature novel halogen‐bonding motifs like double two‐point recognition and infinite chains or networks based on two‐point interactions. Induced conformational changes in the double bidentate halogen‐bond donors could be exploited to obtain different 1D and 2D networks. All solid‐state studies were accompanied by DFT calculations to predict and rationalize the outcome.
Side‐on‐End‐on Coordination of Dinitrogen on a Polynuclear Vanadium Nitride Cluster Anion [V5N5]−
Authors: Cheng Xin , Li Zi‐Yu , Mou Li‐Hui , Ren Yi , Liu Qing‐Yu , Ding Xun‐Lei , He Sheng‐Gui 
Published: October 2019

AbstractThe side‐on‐end‐on coordination of N2 can be very important to activate and functionalize this very stable molecule. However, such coordination has rarely been reported. This study reports a gas‐phase species (a polynuclear vanadium nitride cluster anion [V5N5]−) that can capture N2 efficiently (12 %), and the quantum chemistry modelling suggests an unusual side‐on‐end‐on coordination. The cluster anions were generated by laser ablation and the reaction with N2 has been characterized by mass spectrometry, photoelectron imaging spectroscopy, and density functional theory calculations. The back‐donation interactions between the localized d–d bonding orbitals on the low‐coordinated dual metal (V) sites and the antibonding π* orbitals of N2 are the driving forces to adsorb N2 with a high binding energy (about 2.0 eV).
Cyclotriphosphate: A Brief History, Recent Developments, and Perspectives in Synthesis
Authors: Bezold Dominik , Dürr Tobias , Singh Jyoti , Jessen Henning J. 
Published: October 2019

AbstractThere has been a recent upsurge in the study and application of approaches utilizing cyclotriphosphate 1 (cyclo‐TP, also known as trimetaphosphate, TMP) and/or proceeding through its analogues in synthetic chemistry to access modified oligo‐ and polyphosphates. This is especially useful in the area of chemical nucleotide synthesis, but by no means restricted to it. Enabled by new high yielding and easy‐to‐implement methodologies, these approaches promise to open up an area of research that has previously been underappreciated. Additionally, refinements of concepts of prebiotic phosphorylation chemistry have been disclosed that ultimately rely on cyclo‐TP 1 as a precursor, placing it as a potentially central compound in the emergence of life. Given the importance of such concepts for our understanding of prebiotic chemistry in combination with the need to readily access modified polyphosphates for structural and biological studies, this paper will discuss selected recent developments in the field of cyclo‐TP chemistry, briefly touch on ultraphosphate chemistry, and highlight areas in which further developments can be expected.
Quality‐of‐life assessment in French patients with metastatic melanoma in real life
Authors: Kandel Marguerite , Dalle Stéphane , Bardet Aurélie , Allayous Clara , Mortier Laurent , Dutriaux Caroline , Guillot Bernard , Leccia Marie‐Thérèse , Dalac Sophie , Legoupil Delphine , Saiag Philippe , Montaudie Henri , Arnault Jean‐Philippe , Brunet‐Possenti Florence , Grob Jean‐Jacques , DeQuatrebarbes Julie , Beylot‐Barry Marie , Lesimple Thierry , Aubin François , Maubec Eve , Granel‐Brocard Florence , Stoebner Pierre‐Emmanuel , Dupuy Alain , Dreno Brigitte , Michiels Stefan , Lebbe Céleste , Borget Isabelle 
Published: October 2019

BackgroundSignificant progress was recently observed in the treatment of metastatic melanoma (MM). With >50% of patients now reaching a second line of treatment and a significant improvement in the survival rate, an assessment of quality of life (QoL) during the whole course of the disease becomes necessary. The objective of this study was to describe the QoL of patients with MM in France, from their diagnosis of advanced disease to their death, in real life.MethodsQoL data were collected through MelBase, a prospective, French, multicentric cohort dedicated to the follow‐up of adults with MM. QoL was assessed using the EuroQoL‐5D questionnaire and the Functional Assessment of Cancer Treatment (FACT)‐Melanoma questionnaire at the time of study inclusion, every 3 months, and at the time of each treatment change until death. To assess longitudinal changes from baseline to death, mixed‐effect models for repeated‐measures analyses were used to control for baseline covariates.ResultsQoL was assessed in 1435 patients who were included in the study between 2013 and 2018. The median follow‐up was 9.4 months, and 47% of patients died during follow‐up. During first‐line treatment, the model‐based, mean utility score was 0.830 (95% CI, 0.818‐0.843), the mean FACT‐General score was 77.22 (95% CI, 76.23‐78.22), and the mean FACT‐Melanoma score was 129.46 (95% CI, 128.02‐130.90). At the time of a change in treatment line, there was a decrease of −0.027 (95% CI, −0.03, −0.02) in the utility score, −1.82 (95% CI, −1.88, −1.76) in the FACT‐General score, and −2.98 (95% CI, −3.05, −2.91) in the FACT‐Melanoma score compared with first‐line treatment.ConclusionsIn the MelBase cohort, the QoL among patients with MM seems to be fairly stable over the whole disease course, although a small but significant decrease at time therapy is changed is observed.
Prostate cancer mortality and metastasis under different biopsy frequencies in North American active surveillance cohorts
Authors: Lange Jane M. , Laviana Aaron A. , Penson David F. , Lin Daniel W. , Bill‐Axelson Anna , Carlsson Sigrid V. , Newcomb Lisa F. , Trock Bruce J. , Carter H. Ballentine , Carroll Peter R. , Cooperberg Mathew R. , Cowan Janet E. , Klotz Laurence H. , Etzioni Ruth B. 
Published: October 2019

BackgroundActive surveillance (AS) is an accepted means of managing low‐risk prostate cancer. Because of the rarity of downstream events, data from existing AS cohorts cannot yet address how differences in surveillance intensity affect metastasis and mortality. This study projected the comparative benefits of different AS schedules in men diagnosed with prostate cancer who had Gleason score (GS) ≤6 disease and risk profiles similar to those in North American AS cohorts.MethodsTimes of GS upgrading were simulated based on AS data from the University of Toronto, Johns Hopkins University, the University of California at San Francisco, and the Canary Pass Active Surveillance Cohort. Times to metastasis and prostate cancer death, informed by models from the Scandinavian Prostate Cancer Group 4 trial, were projected under biopsy surveillance schedules ranging from watchful waiting to annual biopsies. Outcomes included the risk of metastasis, the risk of death, remaining life‐years (LYs), and quality‐adjusted LYs.ResultsCompared with watchful waiting, AS biopsies reduced the risk of prostate cancer metastasis and prostate cancer death at 20 years by 1.4% to 3.3% and 1.0% to 2.4%, respectively; and 5‐year biopsies reduced the risk of metastasis and prostate cancer death by 1.0% to 2.4% and 0.6% to 1.6%, respectively. There was little difference between annual and 5‐year biopsy schedules in terms of LYs (range of differences, 0.04‐0.16 LYs) and quality‐adjusted LYs (range of differences, −0.02 to 0.09 quality‐adjusted LYs).ConclusionsAmong men diagnosed with GS ≤6 prostate cancer, obtaining a biopsy every 3 or 4 years appears to be an acceptable alternative to more frequent biopsies. Reducing surveillance intensity for those who have a low risk of progression reduces the number of biopsies while preserving the benefit of more frequent schedules.
One‐Step Synthesis of a Coral‐Like Cobalt Iron Oxyhydroxide Porous Nanoarray: An Efficient Catalyst for Oxygen Evolution Reactions
Authors: Li Yao , Zhang Wenqian , Song Zhicui , Zheng Qiaoji , Xie Fengyu , Long Enyan , Lin Dunmin 
Published: October 2019

AbstractThe design of high‐efficiency and cost‐effective electrocatalysts is one of the most crucial factors for facilitating the oxygen evolution reaction (OER). Therefore, we prepared CoxFe1‐xOOH with coral‐like nanonet arrays on carbon cloth (CC) as high‐performance OER catalysts by a facile hydrothermal method. The resulting CoxFe1‐xOOH was characterized by X‐ray diffraction, scanning electron microscopy, transmission electron microscopy, and X‐ray photoelectron spectrometry. When serving as a catalyst electrode in the OER, Co0.875Fe0.125OOH/CC exhibits excellent catalytic activity. Co0.875Fe0.125OOH/CC has a low overpotential of 300 mV at a current density of 20 mA cm−2 (150 mV less than that of FeOOH/CC) and a small Tafel slope of 83 mV dec−1 in 1.0 M KOH in OER. Moreover, it also has a superior electrochemical durability for more than 50 h.
Single Cell Phenotypic Profiling of 27 DLBCL Cases Reveals Marked Intertumoral and Intratumoral Heterogeneity
Authors: Nissen Michael D. , Kusakabe Manabu , Wang Xuehai , Simkin Guillermo , Gracias Deanne , Tyshchenko Kateryna , Hill Ainsleigh , Meskas Justin , Hung Stacy , Chavez Elizabeth A. , Ennishi Daisuke , Aoki Tomohiro , Sarkozy Clementine , Connors Joseph M. , Farinha Pedro , Slack Graham W. , Gascoyne Randy D. , Brinkman Ryan R. , Scott David W. , Steidl Christian , Weng Andrew P. 
Published: October 2019

AbstractDiffuse large B‐cell lymphoma (DLBCL) is the most common histologic subtype of non‐Hodgkin lymphoma and is notorious for its clinical heterogeneity. Patient outcomes can be predicted by cell‐of‐origin (COO) classification, demonstrating that the underlying transcriptional signature of malignant B‐cells informs biological behavior in the context of standard combination chemotherapy regimens. In the current study, we used mass cytometry (CyTOF) to examine tumor phenotypes at the protein level with single cell resolution in a collection of 27 diagnostic DLBCL biopsy specimens from treatment naïve patients. We found that malignant B‐cells from each patient occupied unique regions in 37‐dimensional phenotypic space with no apparent clustering of samples into discrete subtypes. Interestingly, variable MHC class II expression was found to be the greatest contributor to phenotypic diversity. Within individual tumors, a subset of cases showed multiple phenotypic subpopulations, and in one case, we were able to demonstrate direct correspondence between protein‐level phenotypic subsets and DNA mutation‐defined subclones. In summary, CyTOF analysis can resolve both intertumoral and intratumoral heterogeneity among primary samples and reveals that each case of DLBCL is unique and may be comprised of multiple, genetically distinct subclones. © 2019 International Society for Advancement of Cytometry
Cervical cytology reproducibility and associated clinical and demographic factors
Authors: Hwang Hyunsoo , Follen Michele , Guillaud Martial , Scheurer Michael , MacAulay Calum , MacAulay Calum , Staerkel Gregg A. , van Niekerk Dirk , Yamal Jose‐Miguel 
Published: October 2019

AbstractBackgroundAlthough the Pap test has been the standard screening method for cervical precancer/cancer detection, it has been criticized for having a relatively low sensitivity and a low reproducibility between pathologists. There is limited knowledge about inter‐rater agreement and what clinical and demographic factors are associated with disagreements between pathologists reading the same Pap smear.MethodsThis study aimed to assess inter‐ and intra‐ rater agreement of the Pap smear in 1619 cytologic slides with biopsy confirmation, using kappa statistics. Clinical and demographic factors associated with higher odds of inter‐rater agreement were also examined and stratified by histologic diagnosis grade.ResultsUsing a five grade classification system, the overall kappa statistics for total, inter‐rater, and intra‐rater samples were 0.62, 0.57, and 0.88 (unweighted) and 0.83, 0.81, and 0.95 (weighted), respectively. In stratified analyses by histologic grade, total kappas ranged from 0.40 (atypia) to 0.64 (human papilloma virus/CIN 1). Factors such as referral for abnormal Pap test (diagnostic vs screening population), recruiting site, and parity were found to be associated with higher agreement between the two cytologic readings.ConclusionsWe observed relatively higher levels of agreement compared with other studies. However, variability was considerable and agreement was generally moderate, suggesting that cervical screening test accuracy and reproducibility needs to be improved.