bluuebunny/crossref_metadata_2025_split huggingface dataset:
Acceptability of the “MOVEdiabetes” physical activity intervention in diabetes primary care settings in Oman: findings from participants and practitioners
Authors: Alghafri Thamra S , Harthi Saud Al , Farsi Yahya Al , Al-Ajmi Fatma , Craigie Angela M. , Bannerman Elaine , Anderson Annie S. 
Published: March 2020

Abstract
Background Adequate physical activity (PA) is considered essential in diabetes management. However, evidence on the best method of promoting PA within diabetes care is inconclusive. The current work identifies perceptions on the acceptability of Intervention Group Participants (IGP) and Project Officers (POs) about the “MOVEdiabetes” intervention programme aimed at increasing PA in adults with type 2 diabetes in Oman. Methods The “MOVEdiabetes” programme (PA consultations, pedometers and WhatsApp messages) was delivered by the POs (primary health care practitioners) in four primary care centres within a one-year cluster randomised control trial. Recruitment and retention were measured from trial attendance records. Programme satisfaction, appropriateness, and content suitability were assessed using exit surveys for both the IGP (interview based) and POs (self-administered). Open text questions on perceptions to the study programme were also included. Results Participants were randomised to an intervention group (IG, n= 122) or comparison group (CG, n=110). The overall retention rate at three and 12 months was 92.7% [110(90.2%) IG vs 105(95.5%) CG] and 75% [82(67.2%) IG vs 92(83.6%) CG] respectively. Most (87.5%) POs and more than half (59.8%) IGP perceived the programme as acceptable and many reported that they were “very satisfied/satisfied” with the programme (100% PO’s and 86.6% IGP). Two thirds (67.1%) of IGP would recommend the programme to others. PA consultations, use of pedometers and Whatsapp messages were well perceived by all. Participants recommended the inclusion of dietary advice and PA promotion for the general public. Exploring PA facilities within the community was suggested by POs. Conclusions The “MOVEdiabetes” programme achieved a high retention rate and was perceived as satisfactory and appropriate. Results from this study suggest that it is worthwhile exploring the use of the “MOVEdiabetes” programme in clinical practice and further community links.
The transtheoretical model is effective for weight management: A randomized controlled trial Intervention for Weight Management
Authors: de Freitas Patrícia Pinheiro , de Menezes Mariana Carvalho , Santos Luana Caroline dos , Pimenta Adriano Marçal , Ferreira Adaliene Versiani Matos , Lopes Aline Cristine 
Published: March 2020

Abstract
Background: Given the current worldwide epidemic of obesity, interventions with higher impact, such as those carried out in the primary health care (PHC) setting, are needed. Considering that, this study aimed to evaluate the effect of intervention performed according to the stages of change of the transtheoretical model (TTM) for weight management. Methods: Randomized controlled trial in Brazilian PHC that offers free physical exercise and nutrition education. The participants were women aged 20 years or older who were obese or overweight in PHC service. The intervention group (IG, n=51) received the same orientation as the comparison group (CG, n==35) plus individual health counselling based on the TTM aiming weight loss, which lasted six months. The outcome measures were anthropometric, food and nutrient profiles. Inflammatory parameters were evaluated in a random subsample. The inter-group and intra-group differences were evaluated and analysis of covariance (ANCOVA) used to assess intervention effectiveness, using intention-to-treat analysis. Results: There was a difference between groups of -1.4 kg (CI95%: -2.5; -0.3) in body weight after the intervention. About 97% of women in the IG reported benefits of intervention and presented positive changes in diet, biochemical markers and anthropometry. The IG showed a better BMI, resistine and blood glucose result when compared to CG during follow-up. Conclusion: The individualized TTM-based intervention combined with usual care was effective strategy in PHC. These results encouragement of interdisciplinary practices; nevertheless, research to identify additional strategies is needed to address barriers to weight maintenance among obesity low-income women.
The Challenges of Ethical Behaviors for Drug Supply in the Iran Pharmacies by Deontological Approach
Authors: Iranmanesh Mahla , Yazdi-Feyzabadi Vahid , Mehrolhassani Mohammad Hossein 
Published: March 2020

Abstract
Background: Pharmacists as the trustee of pharmacy services must adhere to ethical principles and evaluate their professionalism. Pharmacists may sometimes show different unethical behaviors in their interactions, so it is essential to understand these behaviors. The present study aimed to determine the challenges of ethical behaviors based on a deontological approach in the area of drug supply in pharmacies.Methods: This qualitative content analysis study was conducted in Kerman in 2018. A range of key players in the field of medication supply were selected by snowball sampling to be interviewed. An effort was made to select samples with maximum variation. Exclusion criteria were; having less than 3 years of experience in pharmacy and supervision, not willing to participate in the interview, and canceling the interview for 3 times. The participants in this study consisted of pharmacy technicians (n=5), patients (n=6), pharmacists (n=8), inspectors of insurance companies (n = 4) and inspectors of food and drug administration (n = 3). Data were analyzed by MAXQDA- 10 software using guided content analysis. The principles of “Beauchamp and Childress Ethics” theory including autonomy, beneficence, non-maleficence, and justice were selected as the main principles. Results: Data analysis in this study revealed 8 main categories and 26 subcategories. The main categories included; patient privacy, patient independence, communication principles, patient-centered services, drug supplier, patient harm avoidance, supervision, and distributive, procedural and interactive justice. These categories also had some subcategories, including increasing patient awareness, culturizing prescription and rational drug use, confidentiality and privacy, and pharmacist-patient relationship/communication, which were the biggest ethical challenges in the area of drug supply at pharmacies. Conclusions: According to the deontological approach, two principles of autonomy and beneficence had the greatest challenges. The emphasis of policymakers in health care system should be on patient independence, patient privacy, and patient-centered services. The results of this study can be used as a tool for introducing ethical challenges to policymakers and developing educational contents, charter of professional ethics in pharmacies, and measures of pharmacy accreditation.
HIV patients dying on anti-tuberculosis treatment: are undiagnosed infections still a problem in French Guiana?
Authors: nacher mathieu , Adenis Antoine , Abboud Philippe , Djossou Felix , Demar Magalie , Epelboin Loïc , Couppié Pierre 
Published: March 2020

Abstract
ObjectiveDespite scaling-up testing and antiretroviral treatment in Latin America, advanced HIV remains a significant public health problem. The objective of the present study was look for historical risk factors for death in French Guiana’s HIV cohort taking into account the immunological status, the main opportunistic infections, and their treatment. A retrospective cohort study was conducted on data collected between 1992 and 2008 to look at factors associated with death in a cohort 2323 patients. Results There were 370 deaths for a total 9608 patient-years. Being on tuberculosis treatment was associated with a greater hazard of death. The diagnosis of confirmed tuberculosis, of histoplasmosis, of toxoplasmosis, and pneumocystosis were independently associated with death. Interactions terms between cotrimoxazole treatment and pneumocystosis, or between confirmed tuberculosis and tuberculosis treatment showed a protective treatment-effect. All patients having received antituberculosis treatment (n=347) did not have a final diagnosis of tuberculosis (n=93). For histoplasmosis, 199 patients received antifungal treatment while 141 were diagnosed as having histoplasmosis. The number of patients on antituberculosis drugs was far greater that the number of patients with confirmed tuberculosis, and these patients on treatment without confirmed tuberculosis had a two fold increase in the risk of dying.
Effect of human chorionic gonadotropin injection before frozen embryo transfer on pregnancy outcomes in endometriosis infertility
Authors: Du Yanbo , Yan Lei , Sun Mei , Sheng Yan , Li Xiufang , Feng Zhenhua , Tang Rong 
Published: March 2020

Abstract
Purpose To investigate the effect of hCG in hormone replacement regime for frozen thawed embryo transfer in women with endometriosis. Methods We performed a retrospective, database-searched cohort study. The data of endometriosis patients who underwent frozen embryo transfer between 1/1/2009-31/8/2018 were collected. According to the protocols for frozen embryo transfer cycle, these patients were divided into two groups: Control group(n=305), and hCG group(n=362). And clinical pregnancy rate, live birth rate, early abortion rate, late abortion rate and ectopic pregnancy rate were compared between the two groups. Results There was a significant increase in clinical pregnancy rate in hCG group (56.6% vs. 48.2%, p=0.035) compared to the control group. And the live birth rate in hCG group (43.5% vs. 37.4%, p=0.113) also elevated, but the difference is statistically insignificant. Conclusion hCG administration in hormone replacement regime for FET increase the pregnancy rate in women with endometriosis.
Identification of motives and barriers to physical activity of Polish young mothers
Authors: Soroka Andrzej , Godlewska Agnieszka , Krzęcio-Nieczyporuk Elżbieta , Kozioł Paulina 
Published: March 2020

Abstract
Background The aim of the study was to determine the level of physical activity of young mothers living in rural and urban areas and their free time budget, as well as to indicate motivating factors and barriers encountered during physical activity. Methods In the study a diagnostic survey method was applied, including two survey IPAQ questionnaires – the long version and author’s questionnaire. A representative sample consisted of 1 064 young mothers giving birth in 2017. Student T test and discriminate function analysis were used in statistical analysis. Results The level of physical activity of young mothers from rural areas did not differ from that of young mothers from urban areas. However, certain differences appeared while taking into account different aspects of physical activity. The main motives for participation in physical activity of young mothers from rural areas were to improve their physical condition and reduce pain complaints. Young mothers from towns emphasized such motives as strengthening self-confidence and improving their position in the family. While the main barriers for both group of respondents in physical activity were the lack of money and interest in occasional events. Conclusions The main conclusion that arise from the study is to need to make efforts which aim to reduce the burden of daily duties of young mothers, strengthen family support, strengthen material status of young families, eliminate barriers related to infrastructure especially of villages in order to facilitate the access to physical activity, raise awareness among population on the importance of health education, create specialized educational programs to promote healthy lifestyle, and finally, to introduce systematic monitoring in order to understand determinants of physical activity of young mothers.
Majocchi’s Granuloma on the Forearm Caused by Trichophyton tonsurans in an Immunocompetent Patient
Authors: Zheng Yun-yan , Li Yue , Chen Ming-yan , Mei Qian-yun , Zhang Ru-zhi 
Published: March 2020

Abstract
Majocchi’s granuloma is a rare fungal infection of the dermis. The most frequently identified cause of Majocchi’s granuloma is anthropophilic Trichophyton rubrum , and it is frequently seen on the anterior aspect of the legs of women. Here, we report a case of Majocchi’s granuloma on the forearm, a site that is rarely involved, in a 62-year-old woman who had been bitten by a dog. Histological examination revealed hyphae in the dermis with a diffuse dermal infiltrate consisting of lymphoplasmacytic cells and neutrophils. Mycological examination confirmed the presence of the fungus, Trichophyton rubrum. Therefore, the diagnosis of Majocchi’s granuloma was confirmed by histological and mycological examination. The patient was treated with local moxibustion and itraconazole, 200 mg/day, for 60 days, which promoted a complete regression of the lesions.
Nontuberculous Mycobacterial Endophthalmitis: Case Series and Review Literature
Authors: Pinitpuwadol Warinyupa , Tesavibul Nattaporn , Boonsophon Sutasinee , Sakiyalak Darin , Sarunket Sucheera , Choopong Pitipol 
Published: March 2020

Abstract
Background : To report three cases of nontuberculous mycobacterial (NTM) endophthalmitis following multiple ocular surgeries and to review previous literature of NTM endophthalmitis. Methods : Clinical manifestation and management of patients with NTM endophthalmitis in the Department of Ophthalmology, Faculty of Medicine, Siriraj hospital, Mahidol University, Bangkok, Thailand were described. In addition, a review of previously reported cases and case series from MEDLINE was performed. The clinical information and type of NTM from the previous studies and our cases were summarized. Results : We reported three cases of NTM endophthalmitis caused by M. haemophilum , M. fortuitum and M. abscessus and a summarized review of 95 additional cases previously published. Of 98 patients, there were 85 exogenous endophthalmitis (86.7%) and 13 endogenous endophthalmitis (13.3%). The patients’ age ranged from 13 to 89 years with mean of 61.1±18.7 years with no gender predominance. Exogenous endophthalmitis occurred in both healthy and immunocompromised hosts, mainly caused by cataract surgery (62.4%). In contrast, almost all endogenous endophthalmitis patients were either primary or secondary immunocompromised, previous history of disseminated NTM infection was identified in 38.5% of cases. Rapid growing NTMs were responsible for exogenous endophthalmitis, while endogenous endophthalmitis were commonly caused by slow growers. Initial and final vision were generally worse than 6/60. There were no significant differences of initial and final vision between exogenous and endogenous causes (p=1.000, 0.446, respectively). Conclusions : NTM endophthalmitis is a serious intraocular infection that leads to irreversible loss of vision. The presentation can mimic a chronic recurrent or persistent intraocular inflammation. History of multiple intraocular surgeries or immune-deficiency in patient with chronic panuveitis should raise the practioner’s suspicion of NTM endophthalmitis. Appropriate diagnosis and treatment are important to optimize visual outcome.
Nasal carriage of Staphylococcus aureus in livestock in North of Morocco
Authors: Mourabit Nadira , Arakrak Abdelhay , Bakkali Mohammed , Zian Zeineb , Bakkach Joaira , Laglaoui Amin 
Published: March 2020

Abstract
Background : In Morocco, data of LA-MRSA nasal carriage are still limited. The objectives of this study were to determine for the first time the nasal carriage rate, antimicrobial susceptibility profiles and virulence genes of S aureus isolated from animals and breeders in close contact. Methods : From 2015 to 2016, 480 nasal swab samples were collected from 27 different Livestock areas in Tangier. The antimicrobial susceptibility phenotypes were determined by disk diffusion according to EUCAST 2016. The presence of nuc , mec A and his homologue mec C, lukS/F-PV, and tst genes were determined by PCR for all isolates. Results : The overall S. aureus nasal carriage rate was low in animals (9.97%) and high in breeders (60%) with a statistically significant difference, (OR = 14.321; 95% CI = 7.484- 27.405; p< 0.0001). In general, S. aureus strains were susceptible to the majority of antibiotics and the higher resistance rates were found against tetracycline (16.7% in animals and 10% in breeders). No MRSA was detected in animals and breeders. A high rate of tst and lukS/F-PV genes has been found only in animals (11.9% and 16.7%, respectively). Conclusion : Despite the lower colonization rate of S. aureus and the absence of MRSA strains in our study, S. aureus strains harbored a higher frequency of tst and lukS/F-PV of virulence genes, which is associated to an increased risk of infection dissemination in humans. This highlights the need for implementing adequate approaches for prevention. Further larger and multi-center studies are needed to validate and confirm our findings. Keywords: Staphylococcus aureus , nasal carriage, animals, breeders, MRSA, Morocco
Intra-articular Delivery of Celastrol by Hollow Mesoporous Silica Nanoparticles for pH-sensitive Anti-inflammatory Therapy against Knee Osteoarthritis
Authors: Jin Tian , Wu Di , Liu Xiao-Ming , Xu Jiang-Tao , Ma Bing-Jie , Ji Yun , Jin Yu-Ying , Wu Si-Yin , Wu Tao , Ke Ma 
Published: March 2020

Abstract
Celastrol, like other natural drugs, has proven effective in anti-inflammatory but was limited in clinic because of the poor solubility and side effects induced by low bioavailability. In recent years, hollow mesoporous silica nanoparticles (HMSNs) have received extensive attention in biomedical field due to their outstanding biocompatibility and multiple modification. Osteoarthritis has acidic and inflammatory environment. In this study, celastrol was loaded in HMSNs and capped with chitosan to construct a pH-responsive nanoparticle medicine(CSL@HMSNs-Cs) with high solubility for osteoarthritis intra-articular injection treatment. Methods: The CSL@HMSNs-Cs were assembled and the characteristics were measured. The CSL@HMSNs-Cs was applied in vitro in the chondrocytes collected from rats cartilage tissue and in vivo in the MIA induced knee osteoarthritis rats via intra-articular injection. Cytotoxicity assay, pH-responsive release, pain behavior, MRI, safranin o fast green staining, ELISA and western blot analysis were applied to evaluate the bioavailability and therapeutic effect of CSL@HMSNs-Cs. Results: CSL@HMSNs-Cs was stable due to the protection of the chitosan layers in alkaline environment(pH=7.7) but revealed good solubility and therapeutic effect in acidic environment(pH=6.0). The cytotoxicity assay showed no cytotoxicity at relatively low concentration(200 μg/mL) and the cell viability of chondrocytes stimulated by IL-1β was increased in CSL@HMSNs-Cs group. Paw withdrawal threshold in CSL@HMSNs-Cs group is increased, and MRI and Safranin O Fast Green staining showed improvements in articular surface erosion and joint effusion. The upregulated expression levels of IL-1β, TNF-α, IL-6, MMP-3 and MMP-13 and NF-κB signaling pathway of chondrocytes were inhibited in CSL@HMSNs-Cs group. Conclusion: Hollow mesoporous silica nanoparticles were idea carrier for natural drug with poor solubility and were of high biocompatibility for intra-articular injection. These intra-articular injectable CSL@HMSNs-Cs with improved solubility present a pH-responsive therapeutic strategy against osteoarthritis.
Encapsulation of the dual FLAP/mPEGS-1 inhibitor BRP-187 into acetalated dextran and PLGA nanoparticles improves its cellular bioactivity
Authors: Shkodra-Pula Blerina , Kretzer Christian , Jordan Paul M. , Klemm Paul , Koeberle Andreas , Pretzel David , Banoglu Erden , Lorkowski Stefan , Wallert Maria , Höppener Stephanie , Stumpf Steffi , Vollrath Antje , Schubert Stephanie , Werz Oliver , Schubert Ulrich 
Published: March 2020

Abstract
Background: Dual inhibitors of the 5-lipoxygenase-activating protein (FLAP) and the microsomal prostaglandin E2 synthase-1 (mPGES-1) may exert better anti-inflammatory efficacy and lower risks of adverse effects versus non-steroidal anti-inflammatory drugs. Despite these advantages, many dual FLAP/mPGES-1 inhibitors are acidic lipophilic molecules with low solubility and strong tendency for plasma protein binding that limit their bioavailability and bioactivity. Here, we present the encapsulation of the dual FLAP/mPGES-1 inhibitor BRP-187 into the biocompatible polymers acetalated dextran (Acdex) and poly(lactic-co-glycolic acid) (PLGA) via nanoprecipitation. Results: The nanoparticles containing BRP-187 were prepared by the nanoprecipitation method and analyzed by dynamic light scattering regarding their hydrodynamic diameter, by scanning electron microscopy for morphology properties, and by UV-VIS spectroscopy for determination of the encapsulation efficiency of the drug. Moreover, we designed fluorescent BRP-187 particles, which showed high cellular uptake by leukocytes, as analyzed by flow cytometry. Finally, BRP-187 nanoparticles were tested in human polymorphonuclear leukocytes and macrophages to determine drug uptake, cytotoxicity, and efficiency to inhibit FLAP and mPGES-1.Conclusion: Our results demonstrate that encapsulation of BRP-187 into Acdex and PLGA is feasible, and both PLGA- and Acdex-based particles loaded with BRP-187 are more efficient in suppressing 5-lipooxygenase product formation and prostaglandin E2 biosynthesis in intact cells as compared to the free compound, particularly after prolonged preincubation periods.
Comparative genomic analysis of Flavobacteriaceae: insights into carbohydrate metabolism, gliding motility and secondary metabolite biosynthesis.
Authors: Gavriilidou Asimenia , Gutleben Johanna , Versluis Dennis , Forgiarini Francesca , Passel Mark WJ van , Ingham Colin J , Smidt Hauke , Sipkema Detmer 
Published: March 2020

Abstract
BackgroundMembers of the bacterial family Flavobacteriaceae are widely distributed in the marine environment and often found associated with algae, fish, detritus or marine invertebrates. Yet, little is known about the characteristics that drive their ubiquity in diverse ecological niches. Here, we provide an overview of functional traits common to taxonomically diverse members of the family Flavobacteriaceae from different environmental sources, with a focus on the Marine clade. We include seven newly sequenced marine sponge-derived strains that were also tested for gliding motility and antimicrobial activity. ResultsComparative genomics revealed that genome similarities appeared to be correlated to 16S rRNA gene phylogeny, while differences were mostly associated with nutrient acquisition, such as carbohydrate metabolism and gliding motility. The high frequency and diversity of genes encoding polymer-degrading enzymes support the capacity of marine Flavobacteriaceae to utilize diverse carbon sources. Homologs of gliding proteins were widespread among all studied Flavobacteriaceae in contrast to members of other phyla, highlighting the particular presence of this feature within the Bacteroidetes. Notably, not all gliding bacteria formed spreading colonies. Genome mining uncovered a diverse secondary metabolite biosynthesis arsenal of Flavobacteriaceae with high prevalence of gene clusters encoding pathways for the production of antimicrobial, antioxidant and cytotoxic compounds. Antimicrobial activity tests showed, however, that the phenotype differed from the genome-derived predictions for the seven tested strains.ConclusionsOur study elucidates the functional repertoire of marine Flavobacteriaceae and highlights the need to combine genomic and experimental data while using the appropriate stimuli to unlock their uncharted metabolic potential.