bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bio-organic fertilizers stimulate indigenous soil Pseudomonas populations to enhance plant disease suppression
Authors: Tao Chengyuan , Li Rong , Xiong Wu , Shen Zongzhuan , Liu Shanshan , Wang Beibei , Ruan Yunze , Geisen Stefan , Shen Qirong , Kowalchuk George A. 
Published: June 2020

Abstract
Background: Plant diseases caused by fungal pathogen result in a substantial economic impact on the global food and fruit industry. Application of organic fertilizers supplemented with biocontrol microorganisms ( i.e. bioorganic fertilizers) has been shown to improve resistance against plant pathogens at least in part due to impacts on the structure and function of the resident soil microbiome. However, it remains unclear whether such improvements are driven by the specific action of microbial inoculants, microbial populations naturally resident to the organic fertilizer or the physical-chemical properties of the compost substrate. The aim of this study was to seek the ecological mechanisms involved in the disease suppressive activity of bio-organic fertilizers. Results: To disentangle the mechanism of bio-organic fertilizer action, we conducted an experiment tracking Fusarium wilt disease of banana and changes in soil microbial communities over three growth seasons in response to the following four treatments: bio-organic fertilizer (containing Bacillus amyloliquefaciens W19), organic fertilizer, sterilized organic fertilizer and sterilized organic fertilizer supplemented with B. amyloliquefaciens W19. We found that sterilized bioorganic fertilizer to which Bacillus was re-inoculated provided a similar degree of disease suppression as the non-sterilized bioorganic fertilizer across cropping seasons. We further observed that disease suppression in these treatments is linked to impacts on the resident soil microbial communities, specifically by leading to increases in specific Pseudomonas spp.. Observed correlations between Bacillus amendment and indigenous Pseudomonas spp. that might underlie pathogen suppression were further studied in laboratory and pot experiments. These studies revealed that specific bacterial taxa synergistically increase biofilm formation and likely acted as a plant-beneficial consortium against the pathogen. Conclusion: Together we demonstrate that the action of bioorganic fertilizer is a product of the biocontrol inoculum within the organic amendment and its impact on the resident soil microbiome. This knowledge should help in the design of more efficient biofertilizers designed to promote soil function.
The Association between dietary total antioxidant capacity with the risk of helicobacter pylori infection among adult: a case–control study
Authors: Sohouli Mohammadhassan , Haghshenas Niloufar , Fatahi Somaye 
Published: June 2020

Abstract
Objective

Dietary total antioxidant capacity (TAC) has been assumed as a useful tool to assess the relationship between the cumulative antioxidant food capacity and several chronic disorders. The aim of this study was to evaluate the association between DTAC and risk of Helicobacter pylori in a case-control study.
Methods

This case-control study was carried out among 150 patients with Helicobacter pylori and 302 healthy subjects aged 18–55 years. Dietary data were collected using a validated 168- items quantitative food frequency questionnaire. DTAC was calculated based on the ferric reducing-antioxidant power (FRAP) values that reported by US Department of Agriculture.
Results

The mean age and BMI of the study participants were 38.70 years and 25.86, respectively. Compared with control subjects, participants with H pylori significantly were older and had higher BMI and smoking use. Patients with H pylori compared with control individuals had also lower intake of total fiber, vitamin E, vitamin C, vitamin D, total dairy, whole grain and vegetables. Compared with participants in the lowest tertile of DTAC, those in the highest terrtile had a significantly lower OR for H pylori after further adjustment for potential confounders (Model 2: OR, 0.20; 95% CI, 0.10–0.40; P for trend, < 0.001).
Conclusions

Our findings showed that a high DTAC was associated with a reduced risk of Helicobacter pylori in adult, which reflects the increased intake of natural dietary antioxidants to prevent the progression of Helicobacter pylori. However, further studies are needed to investigate the t relation between DTAC and risk of Helicobacter pylori.
Documentation of Traditional Knowledge Associated with Medicinal Animals in West Gojjam Zone of Amhara Region, Ethiopia
Authors: Motbaynor Manaye Misganaw , Tadesse Nigussie Seboka , Gashaw Abiyselassie Mulatu , Hailu Ashenafi Ayenew 
Published: June 2020

Abstract
Background In Ethiopia, Ethnozoology and Zootherapeutic research is very limited and little attention has been given.The study was designed to investigate and document traditional knowledge associated with medicinal animals in West Gojjam Zone of Javitenan, North Achefer, and Bahir Dar Zuria districts.Methods Ethnozoological data were collected using structured questionnaires and use value (UV), informant consensus factor (ICF), fidelity level (FL), preferential ranking, and paired comparison were analyzed.Results A total of 26 animal species and were identified and recorded as a source medicine to treat 33 types of ailments. Animal derived medicines of Bos indicus , Trigona spp . and Apis mellifera were frequently reported species to treat various ailments. Bos indicus , Trigona spp ., Apis mellifera , Hyaenidae carnivora , and Labeobarbus spp . were the most frequent use value reports (84%, 52%, 43%, 37% and 36%) respectively. Informants reported 25 animal parts to treat ailments. Honey and meat took the highest frequency use report followed by purified butter, Milk, Liver, fatty meat and Cheese stored more than 7 year were described with average ICF value of 69%. Maximum degree of consensus was developed for remedy of evil eye (88%) followed by dry cough, swelling and hepatitis 82% each. Use of animal derived medicine from Liotrigona spp . used to relive dry-cough tend to have the highest FL (80%); followed by Labeobarbus spp . for swelling (33%) and Trigona spp . for dry-cough (30%). Paired comparisons revealed that purified butter > 2 year, Cheese > 7 year, honey of Liotrigona spp . (Yetinign Tazma), and honey of Trigona spp . (Tazma) were the most popular and efficacious animal-derived medicines to cure malaria and ranked 1st , 2nd , 3rd and 4th , respectively.Conclusion and recommendation results of this study recorded the most popular animal derived medicine to cure various ailments. Thus, government must strengthen traditional healers with training, club formation, material and financial support and improve means of protecting their intellectual property rights. Moreover, chemists and pharmacists should be undergo a detailed study to identify which chemical constituent of animal and animal product is responsible for the respective treatment of ailments in the study area.
Elastography reduces over-medical procedures in thyroid nodules: a systematic review and meta-analysis
Authors: Yan Kun , Cheng Wei , Ma jiancang , Han donggang , Xu Xin , Chen Yanni , Li Jie , Li Yiming , Wang Zhiliang , Jie Pengyuan 
Published: June 2020

Abstract
Background

To evaluate the diagnostic performance of elastography in the differentiating the malignancy from thyroid nodule.

Methods

The literature published in English or Chinese prior to December 2019 which diagnosed by elastography in thyroid nodules were reviewed and summarized. According the inclusion and exclusion criteria, the elegant theses were reserved. Quality of each study was evaluated by the qu ality a ssessment of d iagnostic a ccuracy s tudies tool (QUADAS). Comprehensive Meta-Analysis Version 2.0 was used to describe primary results and explore homogeneity. Publication bias analysis was evaluated by Review Manager 5.3. Meta-Disc version 1.4 was applied to explore threshold effect, and pool sensitivity and specificity. A Summary ROC curve was constructed to calculate the area under the curve.

Results

As the heterogeneity was mild, we performance on random effect model. The publication bias was negligible. The elastography showed high accuracy in the diagnosis of malignant. The pooled sensitivity and specificity with corresponding 95% confidence interval were 0.80 (0.78, 0.82) and 0.80 (0.79, 0.81) respectively. AUC of sROC was 0.9087. Sensitivity analysis demonstrated that the pooled estimates were stable and reliable.

Conclusions

The elastography has obvious sensitivity and specificity in differentiate malignant from the thyroid nodule. Several different types of elastography could achieve the same aim, but the cut-off value is associate with the purpose of the screen program and the subtypes of the technique.
Self-awareness in the training of health care professionals: Validation of a group self-awareness questionnaire among Balint groups in China
Authors: Fritzsche Kurt , Shi Lili , Wei Jing , Sha Yue , Xie Yongbiao , He Yanling , Tschuschke Volker , Flatten Guido , Leonhart Rainer 
Published: June 2020

Abstract
Background

Group-related learning processes in relation to oneself and in interaction with others play an important role in the training of health care professionals. However, an instrument to measure self-awareness in professional groups is not yet available in China. The aim of the study is to validate a questionnaire on self-awareness processes among Balint groups in China.
Methods

After a Balint group session, the participants were asked to fill out a group self-awareness questionnaire (G-SAQ) and two internationally validated instruments to assess group therapy research, namely, the Group Climate Questionnaire (GCQ) and the Group Relationship Questionnaire (GRQ).
Results

Fully completed questionnaires from n = 650 participants were analysed. Most participants came from Beijing, Guangzhou and Shanghai; most were female; and the average age was 31.3 years old (SD 8.2). The confirmatory and exploratory factor analysis of the G-SAQ did not show good solutions. Therefore, we developed a model with only one general factor and a Cronbach's alpha = .936. Low-to-moderate correlations between the G-SAQ and the previously validated questionnaires (the GCQ and the GRQ) indicated acceptable convergent and discriminant validity. Most of the responses in terms of self-awareness ranged from “something learned” to “relatively a lot learned”. The assessment of the group leaders was correlated with the level of learning effects.
Conclusions

The Chinese version of the G-SAQ has satisfactory reliability and validity among participants of Balint groups. It could be used as a measure of self-awareness processes in the future.
Investigation of a functionalized self-assembling nanopeptide hydrogel loaded with adipose-derived mesenchymal stem cells to promote vascular regeneration
Authors: Liu Zhanao , Tian Ailing , Yi Xin , Fan Lufeng , Shao Wenchong , Wu Han , Sun Nianfeng 
Published: June 2020

Abstract
Objectives: To investigate the transplantation of a functionalized self-assembling nanopeptide hydrogel loaded with adipose-derived mesenchymal stem cells (AD-MSCs) into a rabbit hindlimb ischemia model and to evaluate its effect in promoting vascular regeneration in ischemic tissues.Methods: Functionalized self-assembling nanopeptide hydrogels were synthesized, and the physical and chemical properties of the hydrogels were observed by electron microscopy. Primary AD-MSCs were isolated, cultured, immunophenotyped, induced to differentiate, and verified. The self-assembling nanopeptide hydrogel was combined with mesenchymal stem cells for the three-dimensional culture of AD-MSCs, and the growth characteristics were investigated. Animal models were injected with AD-MSC-loaded self-assembled peptide hydrogel, and the therapeutic effects on arterial ischemia were analyzed.Results: The pore size of the functionalized self-assembling nanopeptide hydrogel was suitable for cell growth. Stem cells had a tendency for migration, differentiation, and angiogenesis in three-dimensional culture. The experimental results of transplantation into the rabbit hindlimb ischemia model showed that the functionalized self-assembling nanopeptide hydrogel loaded with AD-MSCs had better efficacy than AD-MSC transplantation alone.Conclusion: Functionalized self-assembling nanopeptide hydrogels can be used as scaffold materials for three-dimensional culture of AD-MSCs. Functionalized self-assembling peptide hydrogels combined with AD-MSCs have better therapeutic effects than traditional stem cell therapies and can promote vascular regeneration.
Shenfu Injection Improves Cerebral Microcirculation and Reduces Brain Injury in a Porcine Model of Hemorrhagic Shock
Authors: Wu Junyuan , Li Zhiwei , Yuan Wei , Zhang Qiang , Liang Yong , Zhang Mingqing , Qin Hongjie , Li Chunsheng 
Published: June 2020

Abstract
Background: The aim of this study was to clarify effects of Shenfu infusion (SFI) on cerebral microcirculation and brain injury after hemorrhagic shock (HS).Methods: Twenty-one domestic male Beijing Landrace pigs were randomly divided into three groups: SFI group (SFI, n=8), saline group (SA, n=8) or sham operation group (SO, n=5). In the SFI group, animals were induced to HS by rapid bleeding to a mean arterial pressure of 40 mmHg within 10 minutes and maintained at 40 ± 3 mmHg for 60 minutes. Volume resuscitation (shed blood and crystalloid) and SFI were given after 1 hour of HS. In the SA group, animals received the same dose of saline instead of SFI. In the SO group, the same surgical procedure was performed but without inducing HS and volume resuscitation. The cerebral microvascular flow index (MFI), nitric oxide synthase (NOS) expression, aquaporin-4 expression, interleukin 6, tumor necrosis factor-α (TNF-α) and ultrastructural of microvascular endothelia were measured.Results: Compared with the SA group, SFI significantly improved cerebral MFI after HS. SFI up regulated cerebral endothelial NOS expression, but down regulated interleukin 6, TNF-α, inducible NOS and aquaporin-4 expression compared with the SA group. The cerebral microvascular endothelial injury and interstitial edema in the SFI group were lighter than those in the SA group.Conclusions: Combined application of SFI with volume resuscitation after HS can improve cerebral microcirculation and reduce brain injury.
Outcome of hamstring graft with preserved insertion: Prospective Study with 5 year Follow-up
Authors: Gupta Ravi , Singhal Akash , Mehta Rohil , David Gladson 
Published: June 2020

Abstract
BackgroundAutologous hamstring graft remains to be a commonly used graft for Anterior Cruciate Ligament (ACL) in sportspersons. With less graft failure rates, better mechanical stability and proprioception with preserved insertion hamstring graft, we investigated long term outcomes of hamstring graft with preserved insertion. Methods: 441 sportspersons were enrolled in the study. They were divided into two groups by computerized randomization. In Group I, ACL reconstruction was done using hamstring free graft (STGF), and in Group II, ACL reconstruction was done using hamstring graft with preserved tibial insertion (STGPI). Postoperatively, patients were assessed for graft rupture, knee stability, Lysholm score, tegner activity and WOMAC score.ResultsThe average age of the patients was 24 ± 2 years in group 1 and 27 ± 8.5 years in group 2. At 5 years, the mean KT-1000 difference was 2.01 in group 1 and 1.96 in group 2 (P = 0.77); the mean Lysholm score was 97.80 and 98.60(p = 0.07), respectively; mean WOMAC score was 3.09 and 3.12(p = 0.89) respectively; mean difference between the pre-injury and post-surgery Tegner level of sports activity was 1.78 and 0.54(P < 0.05), respectively and graft failure rate was 4.1% and 7.7%.(p < 0.05), respectively.ConclusionWe report STGPI to be a more viable option than hamstring free graft in sportspersons for ACL reconstruction due to less graft failure rates and better tegner activity score postoperatively. Further, STGPI is an option with low graft failure rate like that of Bone Patellar Tendon Bone graft,with added advantage of not having significant donor site morbidity.Level of EvidenceLevel 1, Randomized Controlled Trial
Prognostic Significance of Pretreatment Albumin to Alkaline Phosphatase Ratio in Human Cancers: A Meta-Analysis
Authors: Wang Yanwen , Sun Yuwen , Xu Wenying , Wang Yan 
Published: June 2020

Abstract
Purpose: To investigate the prognostic value of pretreatment albumin to alkaline phosphatase ratio (AAPR) in human cancers.Methods: Several electronic databases were searched up to Jan 4, 2020 for relevant studies. The prognostic value of AAPR were assessed by pooled hazard ratios (HRs) with their corresponding 95% confidence intervals (CIs). The endpoint events included the overall survival (OS), disease-free survival (DFS), cancer-specific survival (CSS) and progression-free survival (PFS).Results: A total of 15 articles involving 20 studies with 6062 cancer patients were included. Our results proved that low pretreatment AAPR was related with poor OS (HR=1.83, 95% CI: 1.66-2.02; P＜0.001), DFS (HR=1.97, 95% CI: 1.49-2.61; P＜0.001), CSS (HR=1.88, 95% CI: 1.37-2.56; P＜0.001) and PFS (HR=1.74, 95% CI: 1.24-2.43; P=0.001). In addition, the significant correlation between pretreatment AAPR and OS was not affected by the treatment strategy and tumor pathological type.Conclusion: Low pretreatment AAPR is related to poor prognosis in human cancers, and AAPR could be served as a promising prognostic indicator in cancer patients.
Clinical Outcomes and Prognostic Factors of Arthroscopic Bankart Repair for Recurrent Shoulder Instability in Soldiers: A Retrospective Single-Center Analysis
Authors: Li Ji , Qi Wei , Zhang Qiang , Wang Ketao , Li Wei , Wang Haoran , Li Zhongli 
Published: June 2020

Abstract
Background: The optimal surgical treatment of recurrent traumatic anterior shoulder instability remains a debated topic. The soldier is a special population with high-intensity military training of shoulder. The purpose of this study was to evaluate the clinical outcomes of arthroscopic Bankart repair for recurrent shoulder instability in soldiers and identify the related risk factors. Methods: A retrospective single-center analysis was performed for soldiers with recurrent shoulder instability who underwent arthroscopic Bankart repair between 2009 and 2014. The primary outcome measure was recurrence of instability (redislocation or subluxation). Statistical analysis by binary logistic regression analysis was performed for the significance of various risk factors including sex, gender, number of preoperative dislocations (single/recurrent), the time interval between the first dislocation and the surgery (less or more than 6 months), SLAP repair and number of anchors. The functional outcomes were assessed with the visual analog scale (VAS), the American Shoulder and Elbow Surgery Score (ASES) and Rowe stability score preoperatively and at the last follow-up. Results: A total of 95 shoulders were followed for 53.81±9.37 months (range, 38 to 92 months). The overall postoperative recurrent instability rate was 16.84% (16 in 95). The statistical analysis showed significant differences in age (P=0.045, OR=0.818), and the time interval between the first dislocation and surgery (P=0.035, OR=5.289). There was a significant improvement in the mean VAS, ASES, and Rowe scores postoperatively (P<0.05), but the mean VAS and Rowe scores were lower in the patients of recurrence (P=0.022 and 0.034, respectively). Conclusions: This study suggested that surgery within 6 months of the first dislocation may be required for the young soldiers, although several repair techniques exist for anterior shoulder instability, arthroscopic Bankart repair remains a viable option.
Life-threatening cardiogenic shock in a pediatric patient with SARS-CoV-2-associated myocarditis treated with remdesivir: a case description and report of similar cases from the Literature
Authors: Molinari Silvia , Cola Lucia M.D. , Melzi Maria L. , Cattoni Alessandro , Panceri Roberto , Bombino Michela , Lapadula Giuseppe , Biondi Andrea 
Published: June 2020

Abstract
BackgroundChildren are relatively spared from Coronavirus disease 2019 (COVID-19), but some severe cases have been reported. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection in children may affect the cardiovascular system. We hereby report about a case of myocarditis evolving to cardiogenic shock in a SARS-CoV-2 positive child.Case presentationAn otherwise healthy 12-year-old patient was admitted with fever, vomiting, diarrhoea and drowsiness, without any respiratory symptoms. He was diagnosed with COVID-19 on nasopharyngeal swab. He developed hypotension and cardiogenic shock. Bedside echocardiography revealed left ventricular impairment with an ejection fraction (LVEF) below 25%. Plasmatic markers of myocardial injury were remarkably raised, as well as inflammatory biomarkers, including procalcitonin (highest recorded value: 66 ng/mL) and interleukin-6 (8209 pg/mL). The child was transferred to Intensive Care Unit and he was treated with catecholamine support, mechanical ventilation and empiric anti-infectious therapy, including broad spectrum antibiotics and the antiviral agent remdesivir. All additional microbiological investigations yielded negative results. We observed a gradual improvement of LVEF within 5 days. A cardiac magnetic resonance confirmed the suspicion of myocarditis. After 21 days of hospitalisation, the child was discharged without sequelae.ConclusionsOur hypothesis is that the child suffered from SARS-CoV-2-induced fulminant myocarditis, probably in the setting of cytokine release syndrome (CRS). The peculiarity of this SARS-CoV-2 infection is the presence of cardiac failure in a previously healthy child without a respiratory illness. The positive outcome is in line with published Literature about the overall better prognosis of COVID-19 children compared to adults. Remdesivir, an investigational antiviral therapy, may have played a role on the clinical improvement of the child.
13C-caffeine breath test results show high dependence of aryl-hydrocarbon receptor SNPs
Authors: Ishii Mchiko , Ishii Yukimoto , Nakayama Tomohisa , Takahashi Yasuo , Asai Satoshi 
Published: June 2020

Abstract
Aim: We investigated the relationship between trimethyl-13C-caffeine breath test (triCBT) and single nucleotide polymorphisms (SNPs) that are related to caffeine metabolism and consumption.Methods: Subjects were 132 young healthy adults (median 21 years: 101 male, 31 female). Subjects completed a questionnaire that enquired about their smoking status, consumption of caffeinated drinks (including coffee, black tea, green tea), height, weight, and body mass index (BMI). DNA was extracted from saliva, and genotyping was performed using TaqMan® SNP Genotyping for cytochrome P4501A2 rs762551, rs2472297, and aryl-hydrocarbon receptor rs4410790. Trimethyl 13C-caffeine (100 mg) was dissolved in distilled water and administered orally. Subsequently, breath samples were collected every 10 mins for 90 mins. Infrared spectroscopy was used to analyze the amount of 13CO2 in the expired breath, and the sum (Δ13CO2) over 90 min (S90m) was calculated.Results: All subjects had genotype CC for rs2472297. S90m was not significantly different among rs762551 genotypes; however, there was a significant difference in S90m among rs4410790 genotypes. Δ13CO2 was significantly affected by rs4410790 SNPs and smoking. The receiver operating characteristic area under the curve was 0.758 when rs4410790 phenotype C was considered positive. When the cutoff value was set to S90m (23.4 ‰), the sensitivity and specificity were 71.4% and 72.1%, respectively.Conclusions: Our results suggest that caffeine demethylation is affected by rs4410790 SNPs and smoking, and that triCBT can be used to identify SNPs in rs4410790.