bluuebunny/crossref_metadata_2025_split huggingface dataset:
A COI DNA barcoding survey of Pratylenchus species in the Great Plains Region of North America
Authors: Ozbayrak Mehmet , Todd Tim , Harris Timothy , Higgins Rebecca , Powers Kirsten , Mullin Peter , Sutton Lisa , Powers Thomas 
Published: February 2020

Abstract

Pratylenchus species are among the most common plant parasitic nematodes in the Great Plains Region of North America. Our goal was to survey Pratylenchus species diversity across the Great Plains region using a mitochondrial COI DNA barcode. The objectives were to (i) determine species boundaries of the common Pratylenchus species within the region, (ii) assess the host associations of the barcoded Pratylenchus specimens, and (iii) determine Pratylenchus distribution patterns throughout the region. A total of 860 soil samples, primarily associated with eight major crops, were collected from Colorado, Kansas, Montana, Nebraska, North Dakota, and Wyoming. From this total, 246 soil samples provided the majority of 915 individual nematode specimens that were amplified by PCR and sequenced for a 727 to 739 bp region of COI. Maximum likelihood, neighbor-joining, and Bayesian phylogenetic trees all recognized 19 distinct and well-supported haplotype groups. The most common and widespread haplotype group, representing 53% of all specimens was P. neglectus, detected from 178 fields in 100 counties and associated with fields growing wheat, corn, dry beans, barley, alfalfa, sugar beets, potatoes, and a vineyard. The second most prevalent haplotype group was P. scribneri, representing 30% of all specimens and recovered from 104 fields in 45 counties, and most frequently associated with corn. Mixed field populations were encountered in approximately 20% of infested fields, with P. neglectus and P. scribneri most often occurring together in corn-soybean cropping systems. Less frequently encountered from the region were P. hexincisus, P. thornei, P. penetrans, P. alleni, and P. zeae. Eight additional haplotype groups, potentially new Pratylenchus species, were discovered in the survey.
Impact of a conservation agriculture system on soil characteristics, rice yield, and root-parasitic nematodes in a Cambodian lowland rice field
Authors: Suong Malyna , Chapuis Elodie , Leng Vira , Tivet Florent , Waele Dirk De , Thị Huế Nguyễn , Bellafiore Stéphane 
Published: February 2020

Abstract
Rice production in Southeast Asia is significantly affected by root-parasitic nematodes (RPN). The Green Revolution has encouraged new agricultural practices (e.g. intensive monoculture, high yielding rice variety) to respond to the high rice demand; however, these methods have promoted the spread of these pests. The recent banning of chemical nematicides resulted in a need for alternative sustainable control strategies. In the present study, we assessed the effects of a direct-seeding mulch-based cropping system (DMC) vs conventional plough-based tillages (CT) on soil properties, rice yield and RPN communities during a two-year trial in Cambodia. Our results show that on average the population densities of RPN were significantly higher in DMC than in CT. Molecular identification revealed only two RPN species associated with roots: Meloidogyne graminicola, not previously reported from Cambodia, was predominant and was present throughout the plant’s development, whereas Hirschmanniella mucronata was only found at the tillering and milky stages. We conclude that DMC had a significant positive impact on rice yield, despite higher RPN short-term pressure. In order to increase the efficiency of such cropping systems, further studies and an evaluation of the long-term relationships between DMC, the nature of cover crops used, the soil biota including RPN, and rice yield should be conducted.
The efficiency of a flocculent consisting of saw dust and bentonite clay in the removal of Fe and Ni from AMD
Authors: Ntwampe I. O. , Mthembu R. 
Published: February 2020

Abstract
Sets of experiments were conducted by pouring 200 mL of synthetic acid mine drainage (AMD) into five 500 mL glass beakers, dosed with varying quantities of bentonite clay and saw dust interchangeably, mixed at 250 and 100 rpm for 2 and 10 mns respectively. The samples settled for 1 hour after which the pH, concentration, oxidation reduction potential (ORP) and percentage removal of heavy metals were measured. The results show that the removal efficiency of a flocculent on Ni and Fe is effective. The results show that saw dust does not affect the conductivity of a solution. The ORP of the samples with a flocculent with increasing bentonite clay exhibits a higher rate oxidation than that of a flocculent with increasing saw dust. The crystal morphology of the SEM micrographs with 1.5 g bentonite clay and 1.5 g saw dust show three types of structures, i.e. round flocs, elongated and clustered agglomerates which is indicative of high sorption capacity.
Aroma de café: Economía y empresas cafeteras en la Sierra Nevada de Santa Marta
Authors: Viloria De La Hoz Joaquin 
Published: February 2020

En el artículo se estudia la economía cafetera de la Sierra Nevada de Santa Marta y Serranía de Perijá, así como las actividades empresariales que surgieron en torno a esta actividad, desde finales del siglo XIX hasta principios del siglo XXI. El estudio muestra los orígenes del cultivo del café en el Caribe colombiano, destacando tres haciendas y varios los pioneros de la caficultura regional. Una parte considerable de esa colonización iniciada a finales del siglo XIX en la Sierra Nevada y Serranía de Perijá, fue adelantada por empresarios y familias extranjeras, ubicados mayoritariamente en la vertiente Norte de la Sierra Nevada. Haciendas como Jirocasaca, La Victoria y Cincinnati son estudiadas en este artículo con cierta profundidad, así como algunos pioneros del cultivo como Pedro Cothinet, Joaquín de Mier, Francois Dangond, Orlando Flye y las familias Opdenbosch y Weber. Los casos estudiados en este artículo se enmarcan dentro de los postulados teóricos de la historia empresarial (business history y entrepreneurial history), que tienen su origen en la década de 1920 en las universidades de Estados Unidos. Palabras clave: Café, Empresarios cafeteros, Haciendas cafeteras, Sierra Nevada de Santa Marta, Serranía del Perijá
Critical Review on the Chemical Aspects of Cannabidiol (CBD) and Harmonization of Computational Bioactivity Data
Authors: Mastinu Andrea , Ribaudo Giovanni , Ongaro Alberto , Bonini Sara Anna , Memo Maurizio , Gianoncelli Alessandra 
Published: February 2020

:
Cannabidiol (CBD) is a non-psychotropic phytocannabinoid which represents one of the
constituents of the “phytocomplex” of Cannabis sativa. This natural compound is attracting growing
interest since when CBD-based remedies and commercial products were marketed. This review aims to
exhaustively address the extractive and analytical approaches that have been developed for the isolation
and quantification of CBD. Recent updates on cutting-edge technologies were critically examined in
terms of yield, sensitivity, flexibility and performances in general, and are reviewed alongside original
representative results. As an add-on to currently available contributions in the literature, the evolution
of the novel, efficient synthetic approaches for the preparation of CBD, a procedure which is appealing
for the pharmaceutical industry, is also discussed. Moreover, with the increasing interest on the therapeutic
potential of CBD and the limited understanding of the undergoing biochemical pathways, the
reader will be updated about recent in silico studies on the molecular interactions of CBD towards several
different targets attempting to fill this gap. Computational data retrieved from the literature have
been integrated with novel in silico experiments, critically discussed to provide a comprehensive and
updated overview on the undebatable potential of CBD and its therapeutic profile.
Mixed Oleic Acid-Erucic Acid Liposomes as a Carrier for Anticancer Drugs
Authors: Eh Suk Vicit Rizal , Chung Ivy , Misran Misni 
Published: February 2020

Background:Liposomes are mostly known to be prepared from phospholipids and lipids and have a remarkable capacity to encapsulate both lipophobic and lipophilic molecules. However, there is little research on developing fatty acid liposomes for chemotherapy.Objective:We have successfully prepared mixed fatty acid liposomes from two monounsaturated fatty acids, namely oleic acid and erucic acid, which stabilised by DOPEPEG2000. The Critical Vesicular Concentration (CVC) of liposomes was found to be within 0.09 to 0.21 mmol dm-3, with an average particle size of 400 nm.Methods:Encapsulation of various anticancer drugs such as folinic acid, methotrexate, doxorubicin, or irinotecan resulted in Encapsulation Efficiency (%EE) of up to 90%. Using a 3-(4, 5-dimethylthiazol-2- yl)-2,5-diphenyltetrazolium bromide (MTT) assay, the median Inhibitory Concentration (IC50) values of mixed oleic acid-erucic acid encapsulating hydrophilic drugs was remarkably reduced at the end of 24 hours of incubation with the human lung carcinoma cell line A549.Results:The results suggest that mixed oleic acid-erucic acid liposomes are a potential new approach to further develop as an alternative vehicle of various drugs for cancer treatment.
Dynamic Analysis and Control Research of a 3-DOF Hydraulic Driven Parallel Mechanism
Authors: Zhang Bing , Jiang Saike , Jiang Ziliang , Li Jiandong , Zhou Kehong , Liu Feng 
Published: February 2020

Background:The parallel mechanism is widely used in motion simulators, parallel machine tools, medical equipment and other fields. It has advantages of high rigidity, stable structure and high carrying capacity. However, the control strategy and control method are difficult to study because of the complexity of the parallel mechanism system.Objective:The purpose of this paper was to verify the dynamic model of a hydraulic driven 3-DOF parallel mechanism and propose a compound control strategy to broaden the bandwidth of the control system.Methods:The single rigid body dynamic model of the parallel mechanism was established by the Newton Euler method. The feed forward control strategy based on joint space control with inverse kinematic was designed to improve the bandwidth and control precision. The co-simulation method based on MATLAB / SIMULINK and ADAMS was adopted to verify the dynamics and control strategy.Results:The bandwidth of each degree of freedom in the 3-DOF parallel mechanism was used to expand about 10Hz and the amplitude error was controlled below 5%.Conclusion:Based on the designed dynamic model and composite control strategy, the controlled accuracy of the parallel mechanism is improved and the bandwidth of the control system is broadened. Furthermore, the improvements can be made in aspects of control accuracy and real-time performance to compose more patents on parallel mechanisms.
Dari terminologi ke subtansi pendidikan kewarganegaraan: Implikasi terhadap revitalisasi Pancasila
Authors: Kalidjernih Freddy K , Winarno WInarno 
Published: February 2020

Artikel ini mengeksplorasi berbagai istilah (terminologi) yang berkaitan dengan praktik kewarganegaraan atau pendidikan kewarganegaraan yang digunakan di Indonesia setelah runtuhnya rezim Orde Baru. Ia berpendapat bahwa penggunaan istilah tersebut agak serampangan karena mereka kurang dibahas dan diperebutkan di kalangan akademis. Akibatnya, agak membingungkan ketika pemerintah Indonesia berniat untuk memperkenalkan kembali Pendidikan Pancasila baru-baru ini. Makalah ini menyarankan bahwa penggunaan istilah-istilah tersebut secara konsisten dan sistematis akan memungkinkan kita untuk menempatkan Pancasila secara lebih proporsional dalam “peta” Pendidikan Kewarganegaraan Indonesia (Pendidikan Kewarganegaraan, PKn).---------------------------------------------------------------------------------------------------------practices of citizenship or civic education used in Indonesia after the demise of the New Order regime. It argues that the use of the terms has been rather haphazard because they have been less discussed and contested in academic circles. As a result, it was rather confusing when the Indonesian government intended to re-introduce Pancasila Education recently. This paper suggests that consistent and systematic use of such terms will enable us to locate Pancasila more proportionately in the “map” of Indonesia’s Citizenship Education (Pendidikan Kewarganegaraan, PKn).
Dampak sosialisasi empat pilar MPR RI terhadap Pendidikan Pancasila di perguruan tinggi
Authors: Hastangka Hastangka , Armawi Armaidy , Kaelan Kaelan 
Published: February 2020

Penggunaan istilah empat pilar kehidupan berbangsa dan bernegara untuk program sosialisasi telah menimbulkan polemik dan perdebatan di masyarakat. Empat pilar tersebut meliputi Pancasila, UUD 1945, NKRI, dan Bhinneka Tunggal Ika yang dirumuskan oleh MPR RI. Tujuan penelitian ini adalah untuk mendeskripsikan dampak sosialisasi Empat Pilar tersebut terhadap pendidikan Pancasila di perguruan tinggi. Data penelitian diambil dari buku, jurnal ilmiah, artikel surat kabar, dan berita di media online ataupun offline, dan dokumen yuridis, seperti peraturan perundang-undangan. Analisis data menggunakan pendekatan interpretatif, induksi-analitis, semiotik, dan analisis wacana. Hasil penelitian ini menunjukkan bahwa (1) sosialisasi empat pilar berdampak pada ambiguitas pemaknaan dan pemahaman baik dari peserta didik maupun dosen dalam pembelajaran Pancasila sebagai dasar atau pilar kehidupan bernegara dan berbangsa, dan (2) penggunaan istilah empat pilar telah mengacaukan pemahaman dan pengetahuan para peserta didik dalam memahami Pancasila.----------------------------------------------------------------------------------------------------------------------------------------The application of the term “Four Pillars of Life of the People and the Nation” in socialization creates polemic and debate in society. Those four pillars are Pancasila, the 1945 Constitution, the Unitary State of the Republic of Indonesia, and Unity in Diversity, which formulated by the People's Consultative Assembly of the Republic of Indonesia. The objective of this research is to investigate the impact that socialization has on the education of Pancasila in a higher level of education. The research data obtained from books, scientific journals, newspaper articles, other online and offline sources, and legal documents, for instance, legislation. Interpretative, analytic-induction, semiotic, and disclosure approaches applied to the analysis. The result shows that the socialization has immense impacts on 1). The ambiguity toward the meaning and the understanding by both students and lecturers in learning Pancasila as the pillar of the life of the people and the nation, and 2). The use of the term “four pillars” has disrupted the understanding and the knowledge of students in their comprehension toward Pancasila.
Soft skills profile of vocational school students in Yogyakarta City for entering the industrial world
Authors: Wulaningrum Tri , Hadi Samsul 
Published: February 2020

This study aims to determine the profile and how much the level of soft skills of Vocational High School students in Yogyakarta City. This study is survey research. The population of this study was all students grade XI of State Vocational Schools in Yogyakarta City. The sample of this study was 990 students. The data were collected using a questionnaire. The data were then analyzed and presented in a percentage form. The results of the study present that the percentage gain shows the soft skill profile of Vocational High School students in Yogyakarta City in each aspect, namely communication/persuasion skills of 75.19%; performance management skills of 77.22%; self management skills of 70.27%; interpersonal skills of 78.68%; leadership/organizational skills of 77.85%; and cultural skills of 75.60%. Then, the level of soft skills ability of Vocational High School students in Yogyakarta City is generally categorized in medium, which is in the profile range between M-0.5SD and M + 0.5SD (M - 0.5SD x M + 0.5SD).
The level of use of information and communication technology at vocational high school
Authors: Purnamawati Purnamawati , Arfandi Anas , Nurfaeda Nurfaeda 
Published: February 2020

Learning in the 4.0 era uses the concept of 4C (Communication, Collaboration, Critical Thinking, and Problem Solving, Creativity, and Innovation). This concept is linear to the use of information and communication technology (ICT). Technology literacy is involved in all subjects of learning. This phenomenon triggers the teachers, the students, and the school environment to play an active role so that technological progress has meaningfulness in increasing student competence. This study aims to: (1) describe the level of the use of ICT use, (2) determine the factors that influence the use of ICT, and (3) describe the ability of teachers to use ICT in the learning process. This research is a mixed-method with a sequential explanatory strategy. The data collected use questionnaires, interviews, observations, and documentation. The results obtained: (1) the level of ICT utilization in the learning process is at level 4, which means Managed and Measurable. (2) the factors that influence the use of ICT in the learning process in Makassar City Vocational High School based on the Resiliency Maturity Assessment Framework screen components namely data application factors, and (3) the ability teachers in utilizing ICTs in the learning process at Vocational High School in Makassar City meet the criteria very well.
Real-Time Monitoring of Blood Pressure Using Digitalized Pulse Arrival Time Calculation Technology for Prompt Detection of Sudden Hypertensive Episodes During Laryngeal Microsurgery: Retrospective Observational Study
Authors: Park Yong-Seok , Kim Sung-Hoon , Lee Yoon Se , Choi Seung-Ho , Ku Seung-Woo , Hwang Gyu-Sam 
Published: February 2020

Background
Laryngeal microsurgery (LMS) is often accompanied by a sudden increase in blood pressure (BP) during surgery because of stimulation around the larynx. This sudden change in the hemodynamic status is not immediately reflected in a casual cuff-type measurement that takes intermittent readings every 3 to 5 min.

Objective
This study aimed to investigate the potential of pulse arrival time (PAT) as a marker for a BP surge, which usually occurs in patients undergoing LMS.

Methods
Intermittent measurements of BP and electrocardiogram (ECG) and photoplethysmogram (PPG) signals were recorded during LMS. PAT was defined as the interval between the R-peak on the ECG and the maximum slope on the PPG. Mean PAT values before and after BP increase were compared. PPG-related parameters and the correlations between changes in these variables were calculated.

Results
BP surged because of laryngoscopic manipulation (mean systolic BP [SBP] from 115.3, SD 21.4 mmHg, to 159.9, SD 25.2 mmHg; P<.001), whereas PAT decreased significantly (from mean 460.6, SD 51.9 ms, to 405.8, SD 50.1 ms; P<.001) in most of the cases. The change in SBP showed a significant correlation with the inverse of the PAT (r=0.582; P<.001). Receiver-operating characteristic curve analysis indicated that an increase of 11.5% in the inverse of the PAT could detect a 40% increase in SBP, and the area under the curve was 0.814.

Conclusions
During LMS, where invasive arterial catheterization is not always possible, PAT shows good correlation with SBP and may, therefore, have the potential to identify abrupt BP surges during laryngoscopic manipulations in a noninvasive manner.
Impact of a Web-Based Exercise and Nutritional Education Intervention in Patients Who Are Obese With Hypertension: Randomized Wait-List Controlled Trial
Authors: Lisón Juan Francisco , Palomar Gonzalo , Mensorio Marinna S , Baños Rosa M , Cebolla-Martí Ausiàs , Botella Cristina , Benavent-Caballer Vicent , Rodilla Enrique 
Published: February 2020

Background
Internet-based interventions are a promising strategy for promoting healthy lifestyle behaviors. These have a tremendous potential for delivering electronic health interventions in scalable and cost-effective ways. There is strong evidence that the use of these programs can lead to weight loss and can lower patients’ average blood pressure (BP) levels. So far, few studies have investigated the effects of internet-based programs on patients who are obese with hypertension (HTN).

Objective
The aim of this study is to investigate the short- and long-term efficacy, in terms of body composition and BP parameters, of a self-administered internet-based intervention involving different modules and learning techniques aimed at promoting lifestyle changes (both physical activity and healthy eating) in patients who are obese with HTN.

Methods
A randomized wait-list controlled trial design was used. We recruited 105 adults with HTN who were overweight or obese and randomly assigned them to either a 3-month internet-based intervention group (n=55) or the wait-list control group (n=50). We assessed BMI (primary outcome), body fat mass (BFM), systolic (S)BP and diastolic (D)BP, blood glucose and insulin levels, physical activity levels, and functional capacity for aerobic exercise at Time 0 (preintervention) and Time 1 (postintervention). All the patients in the wait-list control group subsequently received the intervention, and a secondary within-group analysis, which also included these participants, was conducted at Time 2 (12-month follow-up).

Results
A 2-way mixed analysis of covariance showed a significant decrease in BMI, BFM, and blood glucose at 3 months in the internet-based intervention group; the effect size for the BMI and BFM parameters was moderate to large, and there was also a borderline significant trend for DBP and insulin. These results were either maintained or improved upon at Time 2 and showed significant changes for BMI (mean difference −0.4, 95% CI −0.1 to −0.6; P=.005), BFM (mean difference −2.4, 95% CI −1.1 to −3.6; P<.001), DBP (mean difference −1.8, 95% CI −0.2 to −3.3; P=.03), and blood glucose (mean difference −2, 95% CI 0 to −4; P=.04).

Conclusions
Implementation of our self-administered internet-based intervention, which involved different learning techniques aimed to promote lifestyle changes, resulted in positive short- and long-term health benefits in patients who are obese with HTN.

Trial Registration
ClinicalTrials.gov NCT03396302; https://clinicaltrials.gov/ct2/show/NCT03396302