bluuebunny/crossref_metadata_2025_split huggingface dataset:
Progesterone at high doses reduces the growth of U87 and A172 glioblastoma cells: Proteomic changes regarding metabolism and immunity
Authors: Altinoz Meric A. , Ucal Yasemin , Yilmaz Muazzez C. , Kiris İrem , Ozisik Ozan , Sezerman Ugur , Ozpinar Aysel , Elmaci İlhan 
Published: June 2020

AbstractWhile pregnancy may accelerate glioblastoma multiforme (GBM) growth, parity and progesterone (P4) containing treatments (ie, hormone replacement therapy) reduce the risk of GBM development. In parallel, low and high doses of P4 exert stimulating and inhibitory actions on GBM growth, respectively. The mechanisms behind the high‐dose P4‐suppression of GBM growth is unknown. In the present study, we assessed the changes in growth and proteomic profiles when high‐dose P4 (100 and 300 µM) was administered in human U87 and A172 GBM cell lines. The xCELLigence system was used to examine cell growth when different concentrations of P4 (20, 50, 100, and 300 µM) was administered. The protein profiles were determined by two‐dimensional gel electrophoresis in both cell lines when 100 and 300 µM P4 were administered. Finally, the pathways enriched by the differentially expressed proteins were assessed using bioinformatic tools. Increasing doses of P4 blocked the growth of both GBM cells. We identified 26 and 51 differentially expressed proteins (fc > 2) in A172 and U87 cell lines treated with P4, respectively. Only the pro‐tumorigenic mitochondrial ornithine aminotransferase and anti‐apoptotic mitochondrial 60 kDa heat shock protein were downregulated in A172 cell line and U87 cell line when treated with P4, respectively. Detoxification of reactive oxygen species, cellular response to stress, glucose metabolism, and immunity‐related proteins were altered in P4‐treated GBM cell lines. The paradox on the effect of low and high doses of P4 on GBM growth is gaining attention. The mechanism related to the high dose of P4 on GBM growth can be explained by the alterations in detoxification mechanisms, stress, and immune response and glucose metabolism. P4 suppresses GBM growth and as it is nontoxic in comparison to classical chemotherapeutics, it can be used as a new strategy in GBM treatment in the future.
Assessment of somatic mutations in urine and plasma of Wilms tumor patients
Authors: Miguez Ana Carolina Kerekes , Barros Bruna D. de Figueiredo , de Souza Jorge E. S. , da Costa Cecília Maria L. , Cunha Isabela Werneck , Barbosa Paula Nicole Vieira P. , Apezzato Maria Lúcia P. , de Souza Sandro J. , Carraro Dirce Maria 
Published: June 2020

AbstractTumor DNA has been detected in body fluids of cancer patients. Somatic tumor mutations are being used as biomarkers in body fluids to monitor chemotherapy response as a minimally invasive tool. In this study, we evaluated the potential of tracking somatic mutations in free DNA of plasma and urine collected from Wilms tumor (WT) patients for monitoring treatment response. Wilms tumor is a pediatric renal tumor resulting from cell differentiation errors during nephrogenesis. Its mutational repertoire is not completely defined. Thus, for identifying somatic mutations from tumor tissue DNA, we screened matched tumor/leukocyte DNAs using either a panel containing 16 WT‐associated genes or whole‐exome sequencing (WES). The identified somatic tumor mutations were tracked in urine and plasma DNA collected before, during and after treatment. At least one somatic mutation was identified in five out of six WT tissue samples analyzed. Somatic mutations were detected in body fluids before treatment in all five patients (three patients in urine, three in plasma, and one in both body fluids). In all patients, a decrease of the variant allele fraction of somatic mutations was observed in body fluids during neoadjuvant chemotherapy. Interestingly, the persistence of somatic mutations in body fluids was in accordance with clinical parameters. For one patient who progressed to death, it persisted in high levels in serial body fluid samples during treatment. For three patients without disease progression, somatic mutations were not consistently detected in samples throughout monitoring. For one patient with bilateral disease, a somatic mutation was detected at low levels with no support of clinical manifestation. Our results demonstrated the potential of tracking somatic mutations in urine and plasma DNA as a minimally invasive tool for monitoring WT patients. Additional investigation is needed to check the clinical value of insistent somatic mutations in body fluids.
Exosomes released from M2 macrophages transfer miR‐221‐3p contributed to EOC progression through targeting CDKN1B
Authors: Li Xiaoduan , Tang Meiling 
Published: June 2020

AbstractIn contrast to other solid tumors within the abdominal cavity, epithelial ovarian cancers (EOCs) tend to undergo peritoneal metastasis. Thus, the peritoneal immune microenvironment is crucial for EOC progression. Previous reports indicate that the main immune cells within the peritoneum are M2 macrophages, specifically tumor‐associated macrophages (TAMs). The communication between TAMs and tumor cells plays an important role in EOC development, and exosomes, acting as micro–message carriers, occupy an essential position in this process. Microarray analyses of exosomes revealed that miR‐221‐3p was enriched in M2 exosomes. Furthermore, miR‐221‐3p suppressed cyclin‐dependent kinase inhibitor 1B (CDKN1B) directly. Thus, miR‐221‐3p contributed to the proliferation and G1/S transition of EOC cells. Additionally, low levels of CDKN1B were associated with EOC progression and poor prognosis. These observations suggest that TAMs‐derived exosomal miR‐221‐3p acts as a regulator of EOC progression by targeting CDKN1B. The results of this study confirm that certain exosomal microRNAs may provide novel diagnostic biomarkers and therapeutic targets for EOC.
Chemical Constituents of the Vietnamese Marine Sponge Gelliodes sp. and Their Cytotoxic Activities
Authors: Ki Dae‐Won , Kodama Takeshi , El‐Desoky Ahmed H. , Wong Chin Piow , Nguyen Hien Minh , Do Kiep Minh , Thai Quang Minh , Ton Nu Lien Huong , Morita Hiroyuki 
Published: June 2020

AbstractA new decenoic acid derivative, gelliodesinic acid, and a naturally new alkaloid, together with three known furanoterpenoids and two known indole alkaloids, were isolated from the MeOH extract of the marine sponge Gelliodes sp. collected in Vietnam. The chemical structures of the isolated compounds were determined by analyses of 1D‐ and 2D‐NMR and MS data and by comparisons of the data with those reported in the literature. The cytotoxicity assay against HeLa, MCF‐7, and A549 cancer cell lines revealed that the three known furanoterpenes exhibited cytotoxic activities with IC50 values ranging from 23.6 to 75.5 μM against the three cell lines, and that 1H‐indole‐3‐carboxylic acid showed cytotoxicity with an IC50 value of 89.2 μM against A549 cancer cell lines.
Complete Photochemical Regulation of 8–17 DNAzyme Activity by Using Reversible DNA Photo‐crosslinking
Authors: Watanabe Yasuha , Fujimoto Kenzo 
Published: June 2020

AbstractThe regulation of DNAzyme activity is an important problem for its in vivo applications. We achieved photochemical regulation of DNAzyme activity by using reversible DNA photo‐crosslinking of 3‐cyanovinylcarbazole (CNVK). The ODN containing CNVK photo‐crosslinked to a pyrimidine base in the complementary strand after a few seconds of photoirradiation, and its photoadduct was split by photoirradiation of another wavelength. The activity of photo‐crosslinked DNAzyme with CNVK was completely inhibited (OFF state). In contrast, after 312 nm irradiation, DNAzyme activity was recovered upon addition of a substrate strand (ON state). In addition, the photo‐crosslinked DNAzyme is prone to enzymatic digestion by exonuclease. This photochemical OFF to ON switching with reversible DNA photo‐crosslinking was regulated at the desired time and position; therefore, it might be possible to use it for in vivo application.
Outcomes of urgent/emergent transcatheter mitral valve repair (MitraClip): A single center experience
Authors: Kovach Christopher P. , Bell Sean , Kataruka Akash , Reisman Mark , Don Creighton 
Published: June 2020

AbstractObjectivesTo describe the outcomes of urgent/emergent transcatheter edge‐to‐edge mitral valve repair (TMVr) and compare the clinical, echocardiographic, and procedural characteristics of survivors and nonsurvivors.BackgroundTMVr is a treatment strategy for select patients with severe primary or secondary mitral regurgitation. However, knowledge regarding outcomes for urgent/emergent TMVr is limited.MethodsAll urgent or emergent TMVr procedures using MitraClip performed at the University of Washington Medical Center between January 2018 and March 2019 were identified and clinical, echocardiographic, hemodynamic, procedural, and outcomes data were obtained by chart review. Outcomes included all‐cause mortality, hospital mortality, procedural success, periprocedural complications, and hospital readmission.ResultsOf the 20 patients who underwent urgent/emergent TMVr, eight were treated for cardiogenic shock (CS), four for acute decompensated heart failure (ADHF) with hypoxemic respiratory failure requiring mechanical ventilation, and eight for ADHF with failure of inpatient medical therapy. Mechanical circulatory support (MCS) was used in six patients; preceding TMVr in three patients and immediately post‐TMVr in three patients. Overall, 30‐day mortality and hospital readmission rates were 21 and 13%, respectively. Over a median 153 days (IQR 20–491) of follow‐up, 10 patients (50%) died. Preprocedure CS, new or ongoing MCS post‐TMVr, refractory respiratory failure post‐TMVr, and acute kidney injury post‐TMVr were associated with mortality.ConclusionsIn a single‐center retrospective analysis, urgent/emergent TMVr in high‐risk patients with ADHF or CS was associated with high short‐term mortality and periprocedural complications. Prospective studies are warranted to inform patient selection and periprocedural management for urgent/emergent TMVr.
The use of MitraClip to prevent posttranscatheter aortic valve replacement left ventricular “suicide”
Authors: Bode Michael F. , Ahmed Andaleeb A. , Baron Suzanne J. , Labib Sherif B. , Gadey Gautam 
Published: June 2020

AbstractPatients with concomitant severe aortic stenosis (AS) and left ventricular outflow tract (LVOT) obstruction undergoing transcatheter aortic valve replacement (TAVR) are at risk for hemodynamic collapse due to a sudden decrease in afterload causing worsening LVOT obstruction. We present a case of an 88‐year‐old female with symptomatic, severe AS, and LVOT obstruction with systolic anterior motion (SAM) of the mitral leaflet in whom alcohol septal ablation was contraindicated secondary to a chronic total occlusion of the right coronary artery that filled retrograde via septal collaterals. MitraClip at the time of TAVR was successfully performed to treat SAM with subsequent stabilization of LVOT gradients despite treatment of the patient's AS. This novel approach may represent a feasible option to prevent hemodynamic complications after TAVR in patients with significant LVOT obstruction secondary to SAM and AS.
The use of hydroxocobalamin for vasoplegic syndrome in left ventricular assist device patients
Authors: Ayers Brian , Wood Katherine , Falvey Jennifer , Bernstein Wendy , Gosev Igor 
Published: June 2020

AbstractWe demonstrate evidence supporting the efficacy of hydroxocobalamin in reducing vasopressor requirements for LVAD patients with refractory vasoplegia. Further study is needed to substantiate these findings and determine its optimal use in practice.
Agranulocytosis‐complicated DRESS with medullar HHV‐6 replication
Authors: Lehericey Margot , Tétart Florence , Carvalho Priscille , Bauvin Olivia , Hervouet Charles , Joly Pascal , Hébert Vivien 
Published: June 2020

AbstractHHV‐6B is the subtype most often involved in the systemic manifestations of the DRESS, but also in myelosuppression in bone marrow transplant. We report a new observation of its myelosuppressive effect: a case of DRESS complicated by agranulocytosis with detectable HHV‐6 RNA in bone marrow.
Bi‐Functional Magnesium Silicate Catalyzed Glucose and Furfural Transformations to Renewable Chemicals
Authors: Kumar Abhinav , Srivastava Rajendra 
Published: June 2020

AbstractBio‐refinery is attracting significant interest to produce a wide range of renewable chemicals and fuels from biomass that are alternative to fossil fuel derived petrochemicals. Similar to petrochemical industries, bio‐refinery also depends on solid zeolite catalysts. Acid‐base catalysis plays pivotal role in producing a wide range of chemicals from biomass. Herein, the Mg framework substituted MTW zeolite is synthesized and explored in the valorisation of glucose and furfural. Bi‐functional (acidic and basic) characteristics are confirmed using pyridine adsorbed FT−IR analysis and NH3 and CO2 temperature‐programmed desorption techniques. Textural properties and morphological information are retrieved from N2‐sorption, X‐ray photoelectron spectroscopy, and electron microscopy. The activity of the catalyst is demonstrated in the selective isomerisation of glucose to fructose in ethanol. Glucose is converted to methyl lactate in high yield using the same catalyst. Further, the bi‐functional activity of this catalyst is demonstrated in the production of fuel precursor by the reaction of furfural and isopropanol. Mg−MTW zeolite exhibits excellent activity in the production of all these chemicals and fuel derivative. The catalyst exhibits no significant loss in the activity even after five recycles. One simple catalyst affording three renewable synthetic intermediates from glucose and furfural will attract significant attention to catalysis researchers and industrialists.
Low‐Temperature Catalytic Hydrogenolysis of Guaiacol to Phenol over Al‐Doped SBA‐15 Supported Ni Catalysts
Authors: Wang Qiuyue , Chen Yufang , Yang Guanheng , Deng Ping , Lu Xinqing , Ma Rui , Fu Yanghe , Zhu Weidong 
Published: June 2020

AbstractSelective hydrogenolysis of aromatic carbon‐oxygen (Caryl−O) bonds is a key strategy for the generation of aromatic chemicals from lignin. However, this process is usually operated at high temperatures and pressures over hydrogenation catalysts, resulting in a low selectivity for aromatics and an extra consumption of hydrogen. Here, a series of Al‐doped SBA‐15 mesoporous materials with different Si/Al molar ratios (Al‐SBA‐15) were prepared via a post‐synthesis method using NaAlO2 as the Al source, and then Al‐SBA‐15 supported Ni catalysts (Ni/Al‐SBA‐15) were prepared by a deposition‐precipitation method using urea as the hydrolysis reagent. The prepared supports and catalysts were extensively characterized using various techniques such as XRD, N2 adsorption/desorption, TEM, 27Al NMR, NH3‐TPD, XPS, H2‐TPR, and pyridine‐FT‐IR, and the catalysts were evaluated in the hydrogenolysis of the Caryl−O bond in guaiacol and lignin derived compounds under mild conditions. The effects of the Si/Al ratio in catalyst and reaction parameters on guaiacol conversion and product distribution were investigated in detail, associated with solvent effect. The incorporation of Al into the framework of SBA‐15 can improve the Lewis acidity and the dispersion of the supported Ni particles and yet modulate the metal‐support interactions, which are propitious to the hydrogenolysis of the Caryl−O bond in guaiacol. The catalyst Ni/Al‐SBA‐15 with a Si/Al molar ratio of 10 shows the best performance with a guaiacol conversion of 87.4 % and a phenol selectivity of 76.9 % under the mild conditions conducted, because of its proper acidity, suitable metal‐support interactions, and high dispersion of the active species. The present study would stimulate research and development in multi‐functional catalysts for the generation of valuable chemicals from biomass.
Hydrodesulfurization Properties of Nickel Phosphide on Boron‐treated Alumina Supports
Authors: Miles Catherine E. , Carlson Tess R. , Morgan Benjamin J. , Topalian Peter J. , Schare Jacob R. , Bussell Mark E. 
Published: June 2020

AbstractThe deep hydrodesulfurization (HDS) properties of nickel phosphide (Ni2P) on boron‐modified alumina (xB−Al2O3) supports having different B contents have been investigated. Ni2P precursors were prepared on the xB−Al2O3 supports using hypophosphite (‐hypo) or phosphate (‐phos) as the P source and were subsequently reduced in flowing hydrogen. The 4,6‐dimethyldibenzothiophene HDS activities and turnover frequencies of the Ni2P/xB−Al2O3 catalysts showed a strong dependence on B loading, with a maximum observed for the hypophosphite‐ and phosphate‐based Ni2P/B−Al2O3 catalysts corresponding to 0.8 wt % and 1.2 wt % B loadings, respectively. Based on XPS and IR spectral measurements, the optimal B loadings corresponded to ∼20 % B2O3 coverage of the γ‐Al2O3 support and coincided with removal of the most basic hydroxyl groups on the alumina surface. At 573 K, a Ni2P/0.8B−Al2O3‐hypo catalyst was 2.5 times more active than a B‐free Ni2P/Al2O3‐hypo catalyst, while a Ni2P/1.2B−Al2O3‐phos catalyst was 8.6 times more active than a B‐free Ni2P/Al2O3‐phos catalyst. Overall, the hypophosphite‐based Ni2P/B−Al2O3 catalysts exhibited higher HDS activities than the phosphate‐based Ni2P/B−Al2O3 catalysts, in part due to smaller Ni2P particle sizes. The optimized catalysts, Ni2P/0.8B−Al2O3‐hypo and Ni2P/1.2B−Al2O3‐phos, were more active than a sulfided Ni−Mo/Al2O3 catalyst.
Facile Synthesis of Ag@PdnL Icosahedral Nanocrystals as a Class of Cost‐Effective Electrocatalysts toward Formic Acid Oxidation
Authors: Wang Wenxia , Chen Zitao , Shi Yifeng , Lyu Zhiheng , Cao Zhenming , Cheng Haoyan , Chi Miaofang , Xiao Kaijun , Xia Younan 
Published: June 2020

AbstractWe report a facile synthesis of Ag@Pd core−shell icosahedral nanocrystals for the development of cost‐effective electrocatalysts toward formic acid oxidation. With 12.4 nm Ag icosahedra serving as seeds, Pd shells of controlled thicknesses in the range of 3.6–5.8 atomic layers are grown by adjusting the experimental parameters. When examined as catalysts toward formic acid oxidation, all the Ag@Pd nanocrystals exhibit enhanced mass activities relative to a commercial Pd/C catalyst, with the Ag@Pd4.2L nanocrystals showing enhanced mass activity that is almost twice that of a commercial Pd/C. The chronoamperometry measurements indicate that all the Ag@Pd/C catalysts are more robust than the Pd/C, with the catalyst based on Ag@Pd4.2L nanocrystals showing a mass activity greater than that of the pristine Pd/C after holding in a mixture of HCOOH and HClO4 at 0.75 V for 1,000 s. We believe that the strategy demonstrated here can also be extended to the development of other types of advanced electrocatalysts.
Oak‐Associated Negativicute Equipped with Ancestral Aromatic Polyketide Synthase Produces Antimycobacterial Dendrubins
Authors: Ishida Keishi , Shabuer Gulimila , Schieferdecker Sebastian , Pidot Sacha J. , Stinear Timothy P. , Knuepfer Uwe , Cyrulies Michael , Hertweck Christian 
Published: June 2020

AbstractAnaerobic bacteria have only recently been recognized as a source of antibiotics; yet, the metabolic potential of Negativicutes (Gram‐negative staining Firmicutes) such as the oak‐associated Dendrosporobacter quercicolus has remained unknown. Genome mining of D. quercicolus and phylogenetic analyses revealed a gene cluster for a type II polyketide synthase (PKS) complex that belongs to the most ancestral enzyme systems of this type. Metabolic profiling, NMR analyses, and stable‐isotope labeling led to the discovery of a new family of anthraquinone‐type polyphenols, the dendrubins, which are diversified by acylation, methylation, and dimerization. Dendrubin A and B were identified as strong antibiotics against a range of clinically relevant, human‐pathogenic mycobacteria.
Photoinduced DNA Lesions in Dormant Bacteria: The Peculiar Route Leading to Spore Photoproducts Characterized by Multiscale Molecular Dynamics**
Authors: Francés‐Monerris Antonio , Hognon Cécilia , Douki Thierry , Monari Antonio 
Published: June 2020

AbstractSome bacterial species enter a dormant state in the form of spores to resist to unfavorable external conditions. Spores are resistant to a wide series of stress agents, including UV radiation, and can last for tens to hundreds of years. Due to the suspension of biological functions, such as DNA repair, they accumulate DNA damage upon exposure to UV radiation. Differently from active organisms, the most common DNA photoproducts in spores are not cyclobutane pyrimidine dimers, but rather the so‐called spore photoproducts. This noncanonical photochemistry results from the dry state of DNA and its binding to small, acid‐soluble proteins that drastically modify the structure and photoreactivity of the nucleic acid. Herein, multiscale molecular dynamics simulations, including extended classical molecular dynamics and quantum mechanics/molecular mechanics based dynamics, are used to elucidate the coupling of electronic and structural factors that lead to this photochemical outcome. In particular, the well‐described impact of the peculiar DNA environment found in spores on the favored formation of the spore photoproduct, given the small free energy barrier found for this path, is rationalized. Meanwhile, the specific organization of spore DNA precludes the photochemical path that leads to cyclobutane pyrimidine dimer formation.
Coordination‐Induced Emission from Tetraphenylethylene Units and Their Applications
Authors: Yu Jian‐Gang , Sun Li‐Ying , Wang Chong , Li Yang , Han Ying‐Feng 
Published: June 2020

AbstractThanks to the potential of aggregation‐induced emission (AIE) phenomena, improved stabilities, and the good selectivity and sensitivity of the chemical responses exhibited by the products, coordination‐driven self‐assembly with tetraphenylethylene (TPE) units has recently received much attention and has been widely investigated for application in chemical sensors, cell imaging agents, light‐harvesting systems, and others. Several reviews have emerged on the topics of AIE chemistry and aggregation‐induced emission luminogen (AIEgen)‐based supramolecular assembles, however, there is still a distinct lack of full overviews of emission enhancement from the viewpoint of metal‐coordination effects. Thus, this minireview offers recent advances that have been made in the design and application of TPE‐based metallacycles, metallacages, metal‐organic frameworks (MOFs) and coordination polymers (CPs).
Shedding light on human cerebral lipofuscin: An explorative study on identification and quantification
Authors: Hakvoort Karlijn , Otto Louise , Haeren Roel , Hoogland Govert , Schijns Olaf , Vink Hans , Klein Dionne , van Zandvoort Marc , Rijkers Kim 
Published: June 2020

AbstractIncreased oxidative stress has been associated with several neurodegenerative diseases such as Alzheimer's disease, but also with neurological diseases sharing pathophysiological pathways like epilepsy. Lipofuscin is a nondegradable end‐product of oxidative stress; its cerebral presence reflects the cumulative amount of oxidative stress the brain has endured. In this study, we have observed prominent autofluorescent particles in the pial arterial wall and in neocortical parenchyma of young, drug‐resistant epilepsy patients (18–28 years old) who underwent resective brain surgery (n = 6), as well as in older control patients (n = 3). With fluorescence spectroscopic imaging, brightfield microscopy, histochemistry and fluorescence lifetime imaging, these autofluorescent particles were identified as the age pigment lipofuscin. An evaluation of these lipofuscin particles using Imaris© software allowed robust quantification, while the 3D properties allowed visualization of the complex configuration. We elaborate on the usefulness of lipofuscin as a marker of cumulative oxidative stress in the brain. Furthermore, we speculate on the observed differences in particle size and density that we found between young patients and older controls, which could imply a role for lipofuscin in the pathophysiology of epilepsy and possibly other neurological diseases.