bluuebunny/crossref_metadata_2025_split huggingface dataset:
Yarrowia lipolytica causes sporadic cases and local outbreaks of infections and colonisation
Authors: Desnos‐Ollivier Marie , Letscher‐Bru Valerie , Neuvéglise Cecile , Dromer Francoise 
Published: April 2020

SummaryBackgroundYarrowia lipolytica belongs to the normal human microbiota but is also found in substrates with high contents in lipids and used in biotechnological processes. It is sometimes reported as human pathogen and especially in catheter‐related candidaemia.ObjectivesTwo apparently grouped cases of infections and/or contamination were reported involving 3 and 9 patients, respectively, in two hospitals. The goal of this study was to design a molecular tool to study the genetic diversity of Y lipolytica and confirm or not the common source of contamination during these grouped cases.MethodsGiven that there is no genotyping method, we used genomic markers assessed on environmental isolates to determine intra‐species relationship. We selected five highly polymorphic intergenic regions, totalling more than 3200 bp and sequenced them for clinical (n = 20) and environmental (n = 14) isolates. Antifungal susceptibility was determined by EUCAST broth microdilution method.ResultsMultiple alignment of the five sequences revealed divergence of 0%‐5.8% between isolates as compared to approximately 0.2%‐0.25% after alignment of whole genomes, suggesting their potential usefulness to establish genetic relatedness. The analysis showed the multiple origins of the isolates. It uncovered two grouped case of fungaemia involving 3 and 2 patients, respectively. It also revealed several unrelated sporadic cases despite their temporal relationship and one probable laboratory contamination by a common yet uncovered source, explaining several consecutive positive cultures without infection. All isolates had high minimal inhibitory concentration (MIC) for flucytosine, the majority (14/34) was susceptible to fluconazole, and all to the other antifungal agents tested.ConclusionThis method could help elucidate cases related to the opportunistic pathogen Y lipolytica.
Developing a conceptual model of pediatric inpatient safety accidents: A mixed methods approach
Authors: Oh Won‐Oak , Kim EunJoo , Im YeoJin , Han Jihee , Kim Mirim 
Published: April 2020

AbstractGiven that injuries to children during periods of hospitalization can lead to more serious health conditions in vulnerable children, preventing pediatric inpatient injuries should be a priority for the pediatric health care profession. This study was conducted to develop a conceptual model of factors affecting pediatric inpatient safety, including all injuries, not just fall injuries, in pediatric units. An explorative sequential mixed methods design was used. Qualitative interviews were conducted with 13 nurses working in pediatrics units and 17 parents of hospitalized children, and then 200 nurses in pediatrics units and parents of hospitalized children completed a self‐report questionnaire. Based on the integration of both qualitative and quantitative data using building and weaving narrative integration, the Pediatric Inpatient Safety Accidents (PISA) model was proposed. The PISA model contains two main domains, the immediate and mediating domain and encompasses the six factors explain of the elements affecting the occurrence and prevention of pediatric inpatient safety accidents. The PISA model can serve as a model to understand child accidents in hospitals as well as to plan customized nursing interventions to prevent child accidents.
Stress and mood of adults with moyamoya disease: A cross‐sectional study
Authors: Yang Yong Sook , Ryu Gi Wook , Yeom Insun , Shim Kyu Won , Choi Mona 
Published: April 2020

AbstractMoyamoya disease in adults is a chronic, progressive disorder characterized by fine collateral vessel networks in the brain. The disorder can lead to negative mood and stress, which, left unresolved, may increase adverse health outcomes. We conducted a cross‐sectional survey to examine stress and mood of adults with moyamoya disease. Participants were recruited at a university hospital in Seoul, Korea. Data were collected through questionnaires and review of participants' electronic medical records. A total of 109 adults participated. Significant correlations were found between perceived stress, anxiety, and depression. Adults with moyamoya disease experience anxiety, depression, and stress related to the risk of cerebral hemorrhage or ischemia, similar to those with other cerebrovascular disease. If negative mood and stress were uncontrolled, those can cuase adverse health outcomes. Health professionals caring for people with moyamoya disease should carefully observe their stress and mood and develop interventions tailored to stages of disease to help them manage. The study results provide baseline information for understanding the level of, and the factors associated with, stress and mood.
Thai trauma nurses’ knowledge of neuroprotective nursing care of traumatic brain injury patients: A survey study
Authors: Promlek Kesorn , Currey Judy , Damkliang Jintana , Considine Julie 
Published: April 2020

AbstractThai trauma nurses play a vital role in neuroprotective nursing care of patients with moderate or severe traumatic brain injury. Nurses' knowledge of the evidence underpinning initial neuroprotective nursing care vital to safe and high‐quality patient care. However, the current state of knowledge of Thai trauma nurses is poorly understood. In this study, we investigated Thai nurses' knowledge of neuroprotective nursing care of patients with moderate or severe traumatic brain injury. Data were collected by a survey, comprising a section on participant characteristics and series of multiple‐choice questions. All registered nurses (n = 22) and nursing assistants (n = 13) from the trauma ward of a regional Thai hospital were invited to participate: the response rate was 100%. Participants had limited knowledge of carbon dioxide monitoring; causes and implications of hypercapnia; mean arterial pressure and cerebral perfusion pressure targets; management of sedatives and analgesics; and management of hyperthermia. Improving their knowledge focusing on knowledge deficits through educational training and implementation of evidence‐based practice is essential to improve the safety and quality of care for Thai patients with moderate or severe traumatic brain injury.
Syndromes with gingival fibromatosis: A systematic review
Authors: Costa Cláudio Rodrigues Rezende , Braz Shélida Vasconcelos , de Toledo Isabela Porto , Martelli‐Júnior Hercilio , Mazzeu Juliana Forte , Guerra Eliete Neves Silva , Coletta Ricardo D. , Acevedo Ana Carolina 
Published: April 2020

AbstractObjectiveThe aim of systematic review was to describe the phenotypes and molecular profiles of syndromes with gingival fibromatosis (GF).MethodsA comprehensive search of PubMed, LILACS, Livivo, Scopus, and Web of Science was conducted using key terms relevant to the research questions and supplemented by a gray literature search. The Methodological Quality and Synthesis of Case Series and Case Reports in association with the Case Series and Prevalence Studies from the Joanna Briggs Institute critical appraisal tools were used for the risk of bias. We followed the PRISMA checklist guidelines.ResultsEighty‐four studies reporting GF as an oral manifestation of a syndrome were identified in this review. Enamel renal syndrome was the most frequently reported syndrome with GF, represented by 54 individuals in 19 studies, followed by Zimmermann–Laband syndrome with 24 individuals in 15 studies and Costello syndrome, which was presented in a case series study with 41 individuals. Among reported cases, other clinical manifestations such as hypertrichosis, ectopic gingival calcification, and cherubism were described.ConclusionsThe results emphasize the need of systematic oro‐dental‐facial phenotyping for future descriptions as well as further molecular analysis in order to better understand the occurrence of syndromic GF.
Confirming pericardial access by using impedance measurements from a micropuncture needle
Authors: John Mathews , Post Allison , Burkland David A. , Greet Brian D. , Chaisson Jordan , Heberton George A. , Saeed Mohammad , Rasekh Abdi , Razavi Mehdi 
Published: April 2020

AbstractBackgroundPericardial access is complicated by two difficulties: confirming when the needle tip is in the pericardial space, and avoiding complications during access, such as inadvertently puncturing other organs. Conventional imaging tools are inadequate for addressing these difficulties, as they lack soft‐tissue markers that could be used as guidance during access. A system that can both confirm access and avoid inadvertent organ injury is needed.MethodsA 21G micropuncture needle was modified to include two small electrodes at the needle tip. With continuous bioimpedance monitoring from the electrodes, the needle was used to access the pericardium in porcine models (n = 4). The needle was also visualized in vivo by using an electroanatomical map (n = 2). Bioimpedance data from different tissues were analyzed retrospectively.ResultsBioimpedance data collected from the subcutaneous space (992.8 ± 13.1 Ω), anterior mediastinum (972.2 ± 14.2 Ω), pericardial space (323.2 ± 17.1 Ω), mid‐myocardium (349.7 ± 87.6 Ω), right ventricular cavity (235.0 ± 9.7 Ω), lung (1142.0 ± 172.0 Ω), liver (575.0 ± 52.6 Ω), and blood (177.5 ± 1.9 Ω) differed significantly by tissue type (P < .01). Phase data in the frequency domain correlated well with the needle being in the pericardial space. A simple threshold analysis effectively separated lung (threshold = 1120.0 Ω) and blood (threshold = 305.9 Ω) tissues from the other tissue types.ConclusionsContinuous bioimpedance monitoring from a modified micropuncture needle during pericardial access can be used to clearly differentiate tissues. Combined with traditional imaging modalities, this system allows for confirming access to the pericardial space while avoiding inadvertent puncture of other organs, creating a safer and more efficient needle‐access procedure.
Medical simulation utilization among pediatric anesthesiology fellowship programs
Authors: Ambardekar Aditee P. , Newell Alana , Blasius Kimberly , Waldrop William B. , Young David A. 
Published: April 2020

AbstractBackgroundSimulation‐based education is a mainstay in education of pediatric anesthesiology trainees. Despite the known benefits, there is variability in its use and availability among various pediatric anesthesiology fellowship programs.AimThe primary aim was to understand the current state of simulation‐based education among pediatric anesthesiology fellowship programs and define barriers that impede the development of an effective simulation program.MethodsThis survey‐based, observational study of simulation activities within United States‐based pediatric anesthesiology fellowship programs was approved by the Institutional Review Boards (IRB) of the authors' institutions. A 35‐question survey was developed in an iterative manner by simulation educators (AA, WW, DY) and a statistician familiar with survey‐based research (AN) using research electronic data capture (REDCap) for tool development and data collation. Descriptive and thematic analyses were performed on the quantitative and qualitative responses in the survey, respectively, and were stratified with small, medium, and large fellowship programs.ResultsForty‐five of 60 (75%) fellowship programs responded to the survey. The presence of a dedicated simulation program director and number of simulation instructors was positively associated with the size of program and years in operation. Dedicated simulation support was variable across programs and was usually present within the larger programs. A positive association also existed for educational activities among all programs mostly based on size of program and years in operation. Protected time was the most commonly cited barrier to having a comprehensive and sustainable simulation program. There was general agreement for establishing a standardized and shared curriculum among fellowship programs. Approximately 70% of simulation programs had no formal simulation instructor training requirement.ConclusionsSimulation‐based curricula are broadly offered by many fellowship programs. Improved collaboration locally, regionally, and nationally may improve educational opportunities for fellowship programs, particularly the small ones. These efforts may begin with the development of a standardized curriculum and formal instructor training programs.
Stem emissions of monoterpenes, acetaldehyde and methanol from Scots pine (Pinus sylvestris L.) affected by tree–water relations and cambial growth
Authors: Rissanen Kaisa , Vanhatalo Anni , Salmon Yann , Bäck Jaana , Hölttä Teemu 
Published: April 2020

AbstractTree stems are an overlooked source of volatile organic compounds (VOCs). Their contribution to ecosystem processes and total VOC fluxes is not well studied, and assessing it requires better understanding of stem emission dynamics and their driving processes. To gain more mechanistic insight into stem emission patterns, we measured monoterpene, methanol and acetaldehyde emissions from the stems of mature Scots pines (Pinus sylvestris L.) in a boreal forest over three summers. We analysed the effects of temperature, soil water content, tree water status, transpiration and growth on the VOC emissions and used generalized linear models to test their relative importance in explaining the emissions. We show that Scots pine stems are considerable sources of monoterpenes, methanol and acetaldehyde, and their emissions are strongly regulated by temperature. However, even small changes in water availability affected the emission potentials: increased soil water content increased the monoterpene emissions within a day, whereas acetaldehyde and methanol emissions responded within 2–4 days. This lag corresponded to their transport time in the xylem sap from the roots to the stem. Moreover, the emissions of monoterpenes, methanol and acetaldehyde were influenced by the cambial growth rate of the stem with 6–10‐day lags.
High temperature inhibits the accumulation of storage materials by inducing alternative splicing of OsbZIP58 during filling stage in rice
Authors: Xu Heng , Li Xiaofang , Zhang Hua , Wang Liangchao , Zhu Zhengge , Gao Jiping , Li Chunshou , Zhu Ying 
Published: April 2020

AbstractHigh temperature (HT) has an adverse effect on rice grain filling by inhibiting the accumulation of storage materials. However, the regulatory mechanism of this inhibition remains unknown. Here, we report that Opaque2 like transcription factor OsbZIP58 is a key factor regulating storage material accumulation under HT. The OsbZIP58 gene promotes expression of many seed storage protein genes and starch synthesis genes while inhibits expression of some starch hydrolyzing α‐amylase genes under HT. The loss of OsbZIP58 function leads to floury and shrunken endosperms and dramatically reduced storage materials in the seeds under HT. HT is found to affect alternative splicing of OsbZIP58, promoting the formation of the truncated OsbZIP58β protein form over the full‐length OsbZIP58α protein form. The OsbZIP58β form has a lower transcriptional activity than the OsbZIP58α form, especially under HT condition. Interestingly, rice varieties with less heat sensitivity have reduced alternative splicing of OsbZIP58. Therefore, OsbZIP58 is a crucial gene in regulating storage material accumulation under HT and lower alternative splicing of OsbZIP58 may contribute to heat tolerance during grain filling.
Association between children's sleep patterns and problematic behaviors at age 5
Authors: Suda Masao , Nagamitsu Shinichiro , Obara Hitoshi , Shimomura Go , Ishii Ryuta , Yuge Kotaro , Shimomura Kunihisa , Kurokawa Michiko , Matsuishi Toyojiro , Yamagata Zentaro , Kakuma Tatsuyuki , Yamashita Yushiro 
Published: April 2020

AbstractBackgroundNight‐shift lifestyles affect children as well as adults, and are associated with sleep and behavioral problems among children. This study aimed to investigate associations among sleep patterns, individual/environmental factors, and problematic behaviors in children at age 5 years.MethodsData for sleep patterns, individual / environmental factors, and problematic behaviors for 8,689 5‐year‐old children were collected from health‐checkup records. Problematic behaviors investigated were anxious behavior (being afraid, difficulty being separated from the mother), developmental behavior (violence, restlessness, rebellious behavior, restrictive diet, stereotypic play), personal habits (thumb‐sucking, nail‐biting, tic, masturbation), and excretory problems. The relationships between sleep patterns (bedtime, sleep duration) and the presence of these behaviors were analyzed. Individual / environmental factors that affected problematic behaviors were statistically identified using a tree‐form model.ResultsLate bedtime and short sleep duration showed significant adverse effects on children's problematic behaviors – odds ratio (OR): 1.07, 95% confidence interval (CI): 1.03–1.11 and OR: 0.92, 95% CI: 0.87–0.97, respectively. Long television watching time, abnormality at birth, and lack of father's support also showed significant adverse effects on problematic behaviors (OR: 2.34, 95% CI: 1.87–2.94), and significantly affected late bedtime and short sleep duration.ConclusionsThere were significant associations among sleep patterns, individual / environmental factors, and problematic behaviors in 5‐year‐old children. Improving children's sleep patterns, reducing the duration of television watching, and improving support from fathers may reduce problematic behaviors.
Health utility and quality of life in pediatric liver transplant recipients
Authors: Bharij Aashiv , Neighbors Katie , Alonso Estella M. , Mohammad Saeed 
Published: April 2020

AbstractTo measure HU and HRQOL in pediatric liver transplant (LT) recipients, a cross‐sectional study of patient‐parent dyads was conducted. Direct HU were assessed in 48 adolescents ≥12 years using SG and TTO techniques. Indirect HU were measured by Health Utility Index 2 and HUI3 for subjects ≥12 years and CHU9D for ≥7 years. Patients reported HRQOL using PedsQL™ GC and PedsQL™ TM. A total of 108 dyads participated (55.6% female; 73.2% Caucasian; 42.6% biliary atresia; 35.2% living donor; 37.0% Medicaid). Mean age at survey was 13.6 ± 3.5 years, and time from LT was 8.9 ± 4.9 years. 61.2% were on monotherapy, 25 (23.2%) had acute rejection within 3 years, and 15 (13.9%) had a biliary obstruction within 5 years. Mean indirect HU and HRQOL scores by child report were lower than norms (P < .001). LRD recipients had higher PedsQL™ GC, PedsQL™ TM, and HUI3 scores (P < .01). HU in pediatric LT recipients are lower than norms. Availability of HU scores for post‐transplant health states will enable measurement of quality‐adjusted life years for future comparative effectiveness studies.
Progesterone modulates adhesion molecules in uterine epithelial cells and in vitro embryo production in buffalo
Authors: Pandey Sriti , Ahmad Bhat Irfan , Kumar Bharti Mukesh , Shabir Uffaq , Ahmad Peer Bilal , Baiju Indu , Sonwane Arvind , Chandra Vikash , Sai Kumar Gutulla , Taru Sharma Gutulla 
Published: April 2020

AbstractThis study was undertaken to evaluate the role of progesterone (P4) in modulation of the expression profile of adhesion‐related molecules in uterine epithelial cells (UECs) and in vitro blastocyst production in buffalo. UECs were isolated from slaughterhouse‐derived uteri by enzymatic treatment, and cells were characterized by immunocytochemistry (ICC) and PCR assays. The well‐characterized UECs were exposed to different concentrations of P4 (0, 0.314, 3.14 and 6.28 ng/ml) along with the basal level of oestradiol for 6 days. Thereafter, the relative mRNA expression of different biomolecules such as mucin 1 (MUC1), osteopontin, integrin alpha (α3, α6 and αV) and beta (β1 and β3) subunits, progesterone receptor (PR) and oestrogen receptor, was evaluated. Further, day 2 post‐insemination embryos were cultured in mSOF supplemented with or without P4. UECs were found positive for cytokeratin expression and negative for vimentin expression. Progesterone treatment significantly enhanced the mRNA expression of most of the transcripts compared with the control group, and correspondingly, the immunofluorescence depicted higher protein expression of all these molecules. Further, the long‐term exposure of UECs to P4 downregulated the expression of PR and, concomitantly, MUC1. Progesterone supplementation to embryo culture medium significantly (p < .05) improved the blastocyst rate. The study demonstrates the role of P4 hormone in modulation of the expression of early implantation‐related biomolecules in uterine epithelial cells; hence, adequate level of steroids is crucial for normal embryo development and its implantation.
Successful transcervical uterine flushing can be performed without or reduced dose of oestradiol benzoate in cervical relaxation protocol in Dorper ewes
Authors: Dias Jenniffer Hauschildt , Pupin Maria Amélia , Duarte Gabriela Saloni , Brair Viviane Lopes , de Paula Cleber Jonas Carvalho , de Sousa Marco Antonio Paula , Batista Ribrio Ivan Tavares Pereira , Souza‐Fabjan Joanna Maria Gonçalves , Oliveira Maria Emília Franco , Fonseca Jeferson Ferreira 
Published: April 2020

AbstractThis study assessed the efficiency of cervical relaxation protocol using none, half or full dose (1.0 mg) of oestradiol benzoate in Dorper ewes subjected to non‐surgical embryo recovery (NSER). Thirty‐six pluriparous ewes received progestogen sponge (60 mg) for 9 days plus eCG administration (300 IU i.m.) 24 hr before sponge removal. Ewes were not mated and were randomly assigned to receive at 16 hr before NSER 37.5 µg d‐cloprostenol i.m. and different doses of oestradiol benzoate: 0.0 mg (0EB group; n = 12); 0.5 mg (0.5EB group; n = 12) or 1.0 mg of oestradiol (1.0EB group, n = 12). All ewes received oxytocin (50 IU) i.v. 20 min before NSER, which was performed 8 days after sponge removal. Corpora lutea were counted by transrectal ultrasonography 24 hr before NSER. After procedure, the ewes were kept in natural breeding period to check their post‐NSER fertility. NSER was performed in 91.7% (33/36) of the animals with overall fluid recovery efficiency over 97% (p > .05). The cervical transposing with Hegar dilator was longer (p < .05) in 0EB (4.2 ± 0.3 min) compared to 0.5EB (1.7 ± 0.3 min) and 1.0EB group (1.5 ± 0.3 min). The cervical transposing with mandrel/catheter was longer (p < .05) in 0EB (2.4 ± 0.5 min) than 1.0EB group (1.3 ± 0.5 min). Overall duration of uterine flushing was 25.4 min with structure recovery rate of 43.5%, with no difference among groups (p > .05). The post‐NSER fertility was higher (p < .05) in 0.0EB (90%) than 0.5EB group (36.4%). In conclusion, NSER can be successfully performed in Dorper ewes by using a cervical relaxation protocol without oestradiol benzoate.