bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Effects of Combining Progressive Tension Sutures, Closed Drain, and Fibrin Sealant in Abdominoplasty Wound After Deep Inferior Epigastric Perforator Flap Harvesting
Authors: Fang Chien-Liang , Changchien Chih-Hsuan , Chen Ming-Shan , Hsu Chin-Hao , Chen Wei-Chen , Yang Hsin-Yi , Lin Yi-Ling 
Published: December 2019

Background
This study is a retrospective review to compare combining progressive tension sutures, closed drain, and fibrin sealant effects on seroma formation, postoperative drainage volume, and hospital stay for abdominoplasty after deep inferior epigastric artery perforator (DIEP) flap. We ever published a 0% seroma rate in abdominoplasty after DIEP flap using progressive tension sutures and closed drain. Massive abdominal wound drainage caused delayed drain removal and increased hospital stay and medical costs.

Methods
We retrospectively evaluated 54 abdominoplasty patients between December 2013 and September 2017; 43 patients used fibrin glue and 11 used progressive tension sutures and closed drain.

Results
Abdominal drainage for the first 3 postoperative days was 84.65 ± 52.95 mL in the fibrin group, with total drainage of 127.70 ± 125.50 mL and 214.45 ± 104.35 mL in the no fibrin group, with total drainage of 350.45 ± 213.58 mL. Drains were removed on postoperative day 6.21 ± 1.44 in the fibrin group and day 9.64 ± 1.96 in the no fibrin group. The association of the first 3 days and total drainage volumes with the drain removal day significantly differed in the fibrin and no fibrin groups. Hospital stay was 9.88 ± 3.55 and 12.45 ± 5.22 days in the fibrin and no fibrin group, respectively, with borderline significant differences.

Conclusions
Donor site abdominoplasty after DIEP flap combining progressive tension sutures, closed drainage, and fibrin glue can prevent seroma occurrence, reduce postoperative abdominal drainage and need for blood transfusion, and achieve early removal of the abdominal drain, shorter hospital stay, and lower medical cost.
Fat Grafting in Patients With Extensive Unilateral Facial Deficiency
Authors: Lo Lun-Jou , Yamaguchi Kazuaki , Niu Lien-Shin , Liao Chun-Hao , Lin Hsiu-Hsia 
Published: December 2019

Purpose
Autologous fat injection is a widely used, simple, and less invasive technique to correct volume deficiency. This study developed a treatment method by using a 3-dimensional (3D) simulation to plan and implement fat injection in patients with an extensive facial deficiency and then validated the accuracy of the method and treatment outcomes.

Methods
Seven patients with a large unilateral facial deficiency receiving autologous fat grafts between 2015 and 2017 were recruited. One patient received repeated treatment. Furthermore, 3D surgical simulation was used to measure the difference between the mirrored image and lesion side. An extra 20% to 30% of fat graft was added. A color map was provided, and contour lines 2 mm deep marked the location of the fat injection. Outcome assessments were then performed, and a 3D symmetry index was defined using the contour lines on the facial surface.

Results
No significant difference was noted between the predicted volume and postoperative fat graft retention (35.7 ± 7.4 and 31.6 ± 9.7 mL, respectively; P = 0.176). A comparison of preoperative (79.5% ± 4.3%) and postoperative (89.0% ± 3.3%) 3D symmetry indexes indicated significantly improved facial symmetry (P = 0.018). Patient-reported outcomes of satisfaction on FACE-Q questionnaires yielded an average score of 62.73, higher than the control score (59.83).

Conclusions
By using the proposed method, we could predict the required fat graft volume; moreover, the contoured map aided accurate surgical implementation. Thus, this method is useful for planning and guiding fat grafting treatment in patients with major unilateral facial deficiency.
Severe Ocular Complications After Blepharoptosis Correction in the Oculopharyngeal Muscular Dystrophy Patient
Authors: Lin Tzu-Yu , Chen Austin D. , Chang Cheng-Hsien , Liang Wen-Chen , Minami Narihiro , Nishino Ichizo , Lai Chung-Sheng 
Published: December 2019

Background
Blepharoptosis correction in oculopharyngeal muscular dystrophy (OPMD) patients may result in severe ocular complications owing to lagophthalmos and ophthalmoplegia. Managing the acute episode to prevent further aggravation of the keratopathy or blindness is of paramount importance.

Methods
A review of the literature for severe chemosis, keratopathy, and corneal ulceration in the patient population was performed using the PubMed database, with key words including ptosis surgery, ptosis correction, ptosis repair, and oculopharyngeal muscular dystrophy. A retrospective review of all patients with blepharoptosis from a single surgeon from September 2009 and May 2017 was performed, selecting those with OPMD who underwent blepharoptosis correction.

Results
Our literature review revealed a total of 15 articles after excluding repeated articles and selecting those meeting our inclusion criteria. A total of 232 OPMD patients underwent blepharoptosis correction. Severe ocular complications were noted in 7 patients, with treatment unspecified. For 9 years, 2 OPMD patients at our institute underwent blepharoptosis correction, with one developing severe acute keratitis, chemosis, and corneal ulceration due to lagophthalmos and ophthalmoplegia. Use of the temporary drawstring tarsorrhaphy and topical eye drop treatment for 2 weeks led to resolution of corneal ulcerations without necessitating further intervention.

Conclusions
Severe ocular complications may occur after blepharoptosis correction in OPMD patients, potentially owing to lagophthalmos and ophthalmoplegia. Temporary drawstring tarsorrhaphy is an effective option to treat these adverse outcomes.
Impact and cost-effectiveness of nonavalent human papillomavirus vaccination in Switzerland: insights from a dynamic transmission model
Authors: Riesen Maurane , Bogaards Johannes A. , Low Nicola , Althaus Christian L. 
Published: December 2019

ABSTRACTAIMIn Switzerland, human papillomavirus (HPV) vaccination has been implemented using a quadrivalent vaccine that covers HPV types 16 and 18, responsible for about 70% of cervical cancer. The average national uptake was 56% in girls by the age of 16 years in 2014–2016. A nonavalent vaccine, covering five additional oncogenic HPV types was recommended at the end of 2018. The primary aim of this study was to assess the impact and cost-effectiveness of introducing the nonavalent HPV vaccine in Switzerland compared with the quadrivalent vaccine.METHODSWe developed a dynamic transmission model that describes the spread of 10 high risk HPV types. We informed the model with Swiss data about sexual behaviour and cervical cancer screening, and calibrated the model to cervical cancer incidence in Switzerland. We modelled the impact of quadrivalent and nonavalent vaccines at the achieved (56%) and national recommended uptake (80%) in girls. We calculated the incremental cost-effectiveness ratio (ICER) between the nonavalent vaccine, the quadrivalent vaccine and no vaccination. We evaluated costs linked to cervical cancer screening, treatment of different disease stages and vaccination in a sensitivity analysis.RESULTSCompared with quadrivalent HPV vaccination in Switzerland at 56% uptake, vaccinating with the nonavalent vaccine would avert 1,175 cervical cancer deaths, 3,641 cases of cervical cancer and 106,898 CIN treatments over 100 years at 56% uptake. Compared with the quadrivalent vaccine, which would prevent an estimated 67% and 72% of cervical cancer cases at 56% and 80% coverage, the nonavalent vaccine would prevent 83% and 89% of all cervical cancers at the same coverage rates. The sensitivity analysis shows that introducing the nonavalent vaccination should improve health outcomes and offers a cost-saving alternative to the quadrivalent vaccine under the current price difference.CONCLUSIONSAll scenarios with quadrivalent and nonavalent vaccination are likely to be cost-effective compared with no vaccination. Switching to the nonavalent vaccine at current and improved vaccination uptake is likely to be cost-saving under the investigated price difference.
Evolutionary stasis of an RNA virus indicates arbovirus re-emergence triggered by accidental release
Authors: Pascall David J , Nomikou Kyriaki , Bréard Emmanuel , Zientara Stephan , Silva Filipe Ana da , Hoffmann Bernd , Jacquot Maude , Singer Joshua B. , De Clercq Kris , Bøtner Anette , Sailleau Corinne , Viarouge Cyrille , Batten Carrie , Puggioni Giantonella , Ligios Ciriaco , Savini Giovanni , van Rijn Piet A. , Mertens Peter PC , Biek Roman , Palmarini Massimo 
Published: December 2019

AbstractThe mechanisms underlying virus emergence are rarely well understood, making the appearance of outbreaks largely unpredictable. Bluetongue virus serotype 8 (BTV-8), an insect-borne virus of ruminants, emerged in livestock in Northern Europe in 2006, spreading to most European countries by 2009 and causing losses of billions of Euros. Though the outbreak was successfully controlled through vaccination by early 2010, puzzlingly a closely-related BTV-8 strain re-emerged in France in 2015, triggering a second outbreak that is still ongoing. The origin of this virus and the mechanisms underlying its re-emergence are unknown. Here, we performed phylogenetic analyses of 164 whole BTV-8 genomes sampled throughout the two outbreaks. We demonstrate consistent clock-like virus evolution during both epizootics but found negligible evolutionary change between them. We estimate that the ancestor of the second outbreak dates from the height of the first outbreak in 2008. This implies that the virus had not been replicating for multiple years prior to its re-emergence in 2015. Given the absence of any known natural mechanism that could explain BTV-8 persistence over this period without replication, we conclude that the second outbreak was most likely initiated by accidental exposure of livestock to frozen material contaminated with virus from approximately 2008. Our work highlights new targets for pathogen surveillance programmes in livestock and illustrates the power of genomic epidemiology to identify pathways of infectious disease emergence.
Cytological abnormalities and pollen abortion in interspecific hybrids ofNicotiana
Authors: Liao Jugou , Chen Zhiyun , Pan Zihui , Niu Yongzhi , Suo Wenlong , Wei Xuemei , Zhen Yunye , Lv Litang , Ma Wenguang , Chen Suiyun 
Published: December 2019

AbstractInterspecific cross breeding introduces superior agronomic traits into cultivated species; however, problematic pollen sterility occurs in the hybrids. Our previous study obtained interspecific hybrids from the cross between a cytoplasmic male sterility line ofNicotiana tabacumandNicotiana alata, and some of the hybrids were pollen sterile. Here, we conducted an in-depth cellular study to understand the cytological mechanism of pollen abortion in these hybrids (F1-D sterile) compared with pollen development in the fertile hybrids (F1-S sterile) from the same cross. The ultrastructure observation showed that the membrane of microspore in F1-D sterile hybrid was deficient at all represented developmental stages. Chromosome behavior during meiosis was studied by carbol fuchsin staining, which indicated that cytomixis, chromosome leakage and asymmetric callose wall deposition occurred with high frequency in the microsporocyte of F1-D sterile. The results of the ultrastructure and 6-diamidino-2-phenylindole (DAPI) analyses also showed that the cytoplasm and nucleus were unstable and extruded from F1-D sterile microspore during the developmental process, leading to mature pollen grains that were vacuous and collapsed in the aperture region. In addition, delayed tapetum degradation was also detected in the anther of F1-D sterile, and might be associated with irregular sporopollenin deposition in the aperture region of F1-D sterile pollen. Genetic unbalance and cytomembrane deficiency might both be responsible for the instability of the chromosome, nuclear and cytoplasm, and resulted in pollen abortion in F1-D sterile hybrids, and irregular tapetum degradation might also be related with pollen sterility.
Seasonal Variation in the Internet Searches for Cancer Recurrence: An Infodemiological Study
Authors: Lou Xiaoqi , Hu Dingtao , Zhang Man , Xie Qiaomei , Zou Yanfeng , Wang Fang 
Published: December 2019

AbstractBackgroundWhile few clinical and epidemiological studies have assessed how seasonality affects cancer recurrence, it has not been studied with the utility of the internet data. In this study, we aim to test whether cancer recurrence presents seasonality on a population level, utilizing internet search query data.MethodsThis infodemiological study used Google Trends to find query data for the term “cancer recurrence” from January 01, 2004, to December 31, 2018 in the USA, the UK, Canada, and Australia. Time series seasonal decomposition and the cosinor analysis were used to analyze and describe the seasonal trends for cancer recurrence.ResultsA general upward trend in UK and northern hemisphere were observed. Statistically significant seasonal trends on “cancer recurrence” in the USA (p=1.33×10−5), the UK (p=0.012), and northern hemisphere (p=5.67×10−7) were revealed by cosinor analysis, with a peak in early summer and nadir in early winter. Besides, a seasonal variation was also found in Australia (p=2.3×10−4), with a peak in late summer and nadir in late winter.ConclusionsThe evidence from internet search query data showed a seasonal variation in cancer recurrence, with a peak in early summer(northern hemisphere)/late summer(southern hemisphere). Besides, the relative search volume of “cancer recurrence” appeared a general upward trend in UK and northern hemisphere in recent years.
Molybdate to prevent the formation of sulfide during the process of biogas production
Authors: Tenti Pietro , Roman Samuele , Storelli Nicola 
Published: December 2019

AbstractThe process of anaerobic digestion producing biogas is an eco-friendly energy source that promotes recycling from waste biomass such as food chain residues, wet waste, wastewater. In this study, we focused on the problem of the sulfide (H2S) produced by the sulfate-reducing bacteria (SRB) in the presence of sulfate residues. This byproduct is dangerous for human health and an issue due to the highly corrosive effect on metallic components. To this purpose, the Molybdate, a sulfate analog, known in the literature to inhibit SRBs by blocking the first enzyme of the metabolic pathway of anaerobic respiration, was applied. The experiments carried out showed that a concentration of 0.3 mM of molybdate was enough to inhibit the SRB in a complex environment of the anaerobically digested sludge (ADS) took from a real biogas producing bioreactor. During the study, we observed the importance of the sulfate concentration sulfate in the system. Indeed, the production of sulfide was stopped only under the threshold ratio value of 1:10 (molybdate: sulfate). In the short term, the addition of molybdate did not alter the production and quality (% of methane) of the biogas, nor the anaerobic microbial community, including SRB population itself.
High Capacity poly(2-oxazoline) formulation of TLR 7/8 agonist extends survival in a chemo-insensitive, metastatic model of Lung Adenocarcinoma
Authors: Vinod Natasha , Hwang Duhyeong , Azam Salma H. , Van Swearingen Amanda E. D. , Wayne Elizabeth , Fussell Sloane C. , Sokolsky-Papkov Marina , Pecot Chad V. , Kabanov Alexander V. 
Published: December 2019

AbstractAbout 40% of the NSCLC patients have Stage IV cancer at the time of diagnosis. The only viable treatment options for metastatic disease are systemic chemotherapy and immunotherapy. Nonetheless, chemoresistance remains a major cause of chemotherapy failure. New immunotherapeutic modalities such as anti-PD1 checkpoint blockade have shown promise; however, response to such strategies is highly variable across patients. Here, we show that our novel poly(2-oxazoline) (POx) based nanomicellar formulation of Resiquimod, an imidazoquinoline TLR 7/8 agonist, had a superior tumor inhibitory effect in a metastatic model of lung adenocarcinoma, relative to anti-PD1 immune checkpoint blockade therapy as well as platinum-based chemotherapy, which is the mainstay of treatment for NSCLC. Investigation of the in vivo immune status following Resiquimod PM (POx micellar formulation of Resiquimod) treatment showed that Resiquimod-based stimulation of antigen-presenting cells in the tumor microenvironment resulted in the mobilization of anti-tumor CD8+immune response. Our study demonstrates the promise of optimally delivered and nano-formulated Resiquimod as a new immunomodulating therapeutic strategy for the treatment of metastatic NSCLC.
Drebrin-like protein regulates body bending ofC. elegansvia suppression of NCA cation leak channels
Authors: Butkevich Eugenia , Weist Peter , Härtter Daniel , Klopfenstein Dieter R. , Garces Renata , Schmidt Christoph F. 
Published: December 2019

AbstractDrebrin-like protein (DBN-1) inC. elegansis an adaptor protein that connects different cellular pathways to the actin cytoskeleton. Using a CRISPR-Cas9 system, we generated a newdbn-1allele, which lacks 80% of C-terminal part of DBN-1. The mutant displays a striking hyper-bending locomotion phenotype and body posture with two times stronger curvature than wild type. We show by atomic force microscopy that the muscle tone of the mutant remains unaffected. Aiming to track down the cause of hyper-bending, we performed genetic epistasis experiments. We found that mutations in the Rho-specific guanine-nucleotide exchange factor (GEF) domain of UNC-73 (Trio), pan-neuronal expression of dominant negative RHO-1 and mutations in NCA (NALCN) cation leak channels all suppressed hyper-bending in thedbn-1mutant. These data indicate that DBN-1 negatively regulates the activity of both NCA-1 and NCA-2 channels, opposing RHO-1 in the non-canonical Gq pathway. We conclude that DBN-1 is an important component of the neuronal signaling cascade that controls the degree ofC. elegansbody bending during locomotion.
Boron alters carboxyl group binding capacity and Al transport pathway to relieve Al toxicity
Authors: Yan Lei , Riaz Muhammad , Liu Jiayou , Liu Yalin , Zeng Yu , Jiang Cuncang 
Published: December 2019

AbstractBoron (B) is indispensable for plant growth and has been reported in the mitigation of aluminum (Al) toxicity in different plants. This study unraveled the efficacy of B in reducing the toxicity of Al to trifoliate orange seedlings in a hydroponic experiment. In the current study, B supply had a positive effect on root length and plant growth-related parameters, and attenuated Al-induced inhibition of plasma membrane H+-ATPase activity. The results of XPS and SEM-EDS revealed that B reduces the Al accumulation in root cell wall (CW), especially acts on pectin fractions (alkali-soluble pectin), accompanied by suppressing the pectin synthesis, inhibiting pectin methylesterase (PME) activity andPMEexpression. Furthermore, B application inhibitsNRAT1expression while increasesALS1expression, which are responsible for restraining Al transport from external cells to the cytoplasm and accelerating Al divert to vacuoles, and the results can be further demonstrated by TEM-EDS analysis. Taken together, our results indicated that B mainly promotes the efflux of H+by regulating the plasma membrane H+-ATPase activity, futhur reduce the demethylation of pectin to weaken Al binding ability to carboxyl. More importantly, B alleviated some of the toxic effects of Al by decreasing the deposition of Al in cytoplasm and compartmentalizes Al into vacuoles.One-sentence summaryBoron can reduce the binding amount of carboxyl group to Al in pectin, decreasing the deposition of Al in cytoplasm and compartmentalizes Al into vacuoles, thereby reduce the toxicity of Al to plants..
Chromatin remodeling in bovine embryos indicates species-specific regulation of genome activation
Authors: Halstead Michelle M , Ma Xin , Schultz Richard M , Ross Pablo J 
Published: December 2019

AbstractThe maternal-to-zygotic transition (MZT) is underpinned by wide-spread transcriptomic and epigenomic remodeling that facilitates totipotency acquisition. Factors regulating MZT vary across species and differences in timing of developmental transitions and motif enrichment at accessible chromatin between human and mouse embryos suggest a distinct regulatory circuitry. Profiling accessible chromatin in bovine preimplantation embryos—timing of developmental transitions in bovine closely resembles that in human—indicated that prior to embryonic genome activation (EGA) accessible chromatin is enriched in maternal transcription factor recognition sites, e.g., CTCF, KLFs, NFY, and SP1, echoing observations in humans and mice, and suggesting that a conserved set of maternal factors regulate chromatin remodeling prior to EGA. In contrast, open chromatin established during EGA was primarily enriched for homeobox motifs and showed remarkable similarities between cattle and humans, indicating that cattle could be a more relevant model for human preimplantation development than mice.
Context-specific control of the neural dynamics of temporal attention by the human cerebellum
Authors: Breska Assaf , Ivry Richard B. 
Published: December 2019

SummaryPhysiological methods have identified a number of signatures of temporal prediction, a core component of attention. While the underlying neural dynamics have been linked to activity within cortico-striatal networks, recent work has shown that the behavioral benefits of temporal prediction causally rely on the cerebellum. Here we examine the involvement of the human cerebellum in the generation and/or temporal adjustment of anticipatory neural dynamics, measuring scalp electroencephalography in individuals with cerebellar degeneration. When the temporal prediction relied on an interval representation, duration-dependent adjustments were impaired in the cerebellar group compared to matched controls. This impairment was evident in ramping activity, beta-band power, and phase locking of delta-band activity. Remarkably, these same neural adjustments were preserved when the prediction relied on a rhythmic stream. Thus, the cerebellum has a context-specific causal role in the adjustment of anticipatory neural dynamics of temporal prediction, providing the requisite modulation to optimize behavior.