bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pathologic Response Rates for Breast Cancer Stages as a Predictor of Outcomes in Patients Receiving Neoadjuvant Chemotherapy Followed by Breast-Conserving Surgery
Authors: Zhang jiaqiang , Lu Chang-Yun , Chen Ho-Min , Wu Szu-Yuan 
Published: October 2020

Abstract
PURPOSE: To determine easy-to-use predictors of overall survival (OS), locoregional recurrence (LRR), and distant metastasis (DM) in patients with breast invasive ductal carcinoma (IDC) receiving neoadjuvant chemotherapy (NACT) followed by breast-conserving surgery (BCS), we obtained pathologic response rates (PRRs) for combined primary and nodal diseases (American Joint Committee on Cancer [AJCC] stages) from clinical and pathologic reports, and we used these as predictors.PATIENTS AND METHODS: We enrolled patients with IDC who had received NACT followed by BCS. Cox regression analysis was used to calculate hazard ratios (HRs) and confidence intervals (CIs) for the patients’ PRRs; other independent predictors were controlled for or stratified in the analysis.RESULTS: We analyzed 1047 patients with IDC (611, 260, and 176 patients in clinical stages IIB, IIIA, and IIIB-C, respectively) receiving NACT and BCS. After multivariate Cox regression analyses, the adjusted HRs (aHRs; 95% CI) in patients with pathologic complete response (ypT0N0) were 0.26 (0.13–0.56), 0.36 (0.15–0.85), and 0.15 (0.08–0.31) for all-cause mortality, LRR, and DM, respectively. The aHRs (95% CI) in patients with downstaging of AJCC stages were 0.55 (0.35–0.89), 0.91 (0.62–0.96), and 0.63 (0.43–0.91) for all-cause mortality, LRR, and DM, respectively. The aHRs (95% CI) in patients with upstaging of AJCC stages were 1.77 (1.06–2.24), 1.08 (1.03–1.82), and 1.19 (1.07–2.01) for all-cause mortality, LRR, and DM, respectively.CONCLUSION: The impacts of AJCC-stage PRRs are useful predictive tools and strong predictors for OS, LRR, and DM in patients with breast IDC receiving NACT followed by BCS.
Comparison the Efficacy of Intranasal Ketamine Versus Intravenous Ketorolac on Acute Non-traumatic Headaches: A Randomized Double-Blind Clinical Trial
Authors: Sarvari Houman Rafiee , Baigrezaii Hamidreza , Nazarianpirdosti Mohammad , Meysami Amirhossein , Safari-Faramani Roya 
Published: October 2020

Abstract
Introduction: Non - traumatic headaches are one of the most common causes of referral to hospital emergency. This study aimed to compare the efficacy of intranasal ketamine and intravenous ketorolac on acute non-traumatic headaches.Methods: This randomized and double-blind clinical trial in 2019 years. 140 people were randomly divided into intranasal ketamine (A) and intravenous ketorolac (B). Group (A) received ketamine intranasal (0.75 mg/kg, max 75mg), and group B received intravenous ketorolac (30 mg). Headache severity was measured on arrival, 30, 60, and 120 minutes after intervention with Visual Analogue Scale (VAS). The side effects were recorded an hour after the intervention.Result: The mean difference of pain intensity 30, 60, and 120 minutes after the intervention between the two groups were statistically significant (p<0.001). In the first 30 minutes, significant changes were observed in the VAS levels of the two groups. These changes were more and significant in the intranasal ketamine group (p <0.001). Side effects such as fatigue, dizziness, public discomfort, nausea, increased heart rate, and hypertension were significantly higher in the ketamine group (p <0.05).Conclusion: Intranasal ketamine and intravenous ketorolac both effectively reduced headaches. However, more analgesic effects of intranasal ketamine in a short time can be considered as a selective approach to reducing headaches.Trial registration: IRCT20180108038276N3, Registered 29 September 2019.
Homelessness and Health: A Retrospective Analysis of Medical Records From 2006 - 2020 to Explore the Health and Social Situation of Homeless People in Berlin. A Study Protocol
Authors: Lech Sonia , Schenk Liane , Castro Jenny De la Torre , Schindel Daniel 
Published: October 2020

Abstract
Background: Homelessness is often described as both a driver and a consequence of poor health, social exclusion and economic marginalisation. The present protocol provides a detailed description of the study Examining the health situation of homeless people in Berlin: a retrospective analysis of data from the health centre for the homeless of the Jenny De la Torre Foundation from 2006 to 2020 (GIG study). The primary objective of the GIG study is to describe and analyse the social and health situation of homeless people in Berlin. Methods: A retrospective secondary data analysis of an anonymous full census of medical records for the years 2006 until 2020 from a health centre for homeless people will be carried out. The main outcome is the description and analysis of the social and health situation of homeless people in Berlin. Total and cross-sectional sample characteristics will be presented in a descriptive analysis using Chi-Square Test, Mann-Whitney-U-Test or independent t-Test as appropriate to test (sub)group differences. Further, outcomes will be analysed using finite mixture modelling in order to distinguish different types of social and health conditions. Latent variable regressions will be applied in order to identify sociodemographic and disease-related factors associated with decreasing health conditions.Discussion: Given the high number of homeless individuals in Germany, it is of great importance to examine their social and health situation in order to gain a better understanding of challenges and needs of homeless people and work on new approaches and solutions to effectively address these.Trail Registration: The study was prospectively registered with the German Clinical Trials Register (trial registration number: DRKS00021172). Registered 26 June 2020, https://www.drks.de/DRKS00021172
The Correlation Between Endotoxin, D-Lactate and Diamine Oxidase With Endoscopic Activity in Inflammatory Bowel Disease
Authors: Zhang Qi , Chen Xi , Chen Min 
Published: October 2020

Abstract
Background & Objective: The disease activity monitoring of inflammatory bowel disease (IBD) plays a crucial role for making therapeutic strategies. Endoscopy has been recognized as a gold standard for evaluating disease activity of IBD. However, this method is invasive. Currently, a noninvasive biomarker that could replace endoscope is needed in clinical practice. In this study, we examined whether the diamine oxidase (DAO), D-lactate and endotoxin (ETX) could monitor the disease activity and predict endoscopic remission in patient with IBD. Methods: A total of 149 eligible IBD patients (82CD and67UC) who had received both endoscopic examination and intestinal barrier function detection in our hospital were enrolled in this study. Endoscopic activity was estimated by the Simple Endoscopic Score (SES-CD) for Crohn’s disease, and the ulcerative colitis endoscopic index of severity (UCEIS) for ulcerative colitis. The predictive value and optimal predictive thresholds for those biomarkers were determined by Receiver operating characteristic analysis.Results: For UC patients, DAO, D-lactate and ETX showed better correlation with UCEIS than ESR and CRP, and exhibited satisfactory predictive value in predicting remission. Among patients with CD, DAO and ETX not only showed a better correlation than ESR and CRP with SES-CD, but also capacity to identify more severe patients.Conclusion: DAO and ETX could be used to distinguish different endoscopic activity of CD. DAO, D-lactate and ETX could predict UC endoscopic remission.
The Effect of Carnitine on the Quality of Life and Sleep-Wake Disturbance in Patients With Liver Cirrhosis
Authors: Miyaaki Hisamitsu , Haraguchi Masafumi , Miuma Satoshi , Fukushima Masanori , Sasaki Ryu , Miyazoe Yuri , Shigeno Masaya , Nakao Kazuhiko 
Published: October 2020

Abstract
Background: Some patients with liver cirrhosis have sleep-wake disturbance and reduced quality of life (QOL). However, the effects of L-carnitine on QOL and sleep-wake disturbance have not been extensively studied. Thus, we aimed to examine the effects of L-carnitine on the sleep quality and QOL in cirrhotic patients.Methods: We investigated 19 cirrhotic patients (age: 65.0±10.2 years, male:female=10:9, Child-Pugh score: 6.5±1.8) treated with 1500-mg L-carnitine at Nagasaki University Hospital and its associated hospitals. We used questionnaires to evaluate the patients’ sleep-wake disturbance and QOL at the time of entry and 8 weeks. Results: Cirrhosis-related symptom scores (CSSs), Pittsburgh Sleep Quality Index (PSQI) and General Health (GH) of QOL score improved with L-carnitine treatment (CSS: from 11.5±9.4 to 9.4±3.7, p=0.015; PSQI: from 9.6±5.8 to 7.4±3.3, p=0.035; GH: from 33.5±8.2 to 38.3±9.8, p=0.05). L-carnitine treatment reduced PSQI in 12 out of 14 patients with sleep disturbance (PSQI>5). CSS was improved in 12 cases (63%), and PSQI was improved in 13 cases (68%). However, L-carnitine treatment did not improve daytime hypersomnolence, as evaluated by the Epworth Sleepiness Scale. None of patients had any adverse events related to the L-carnitine treatment.Conclusions: L-carnitine is a safe and useful treatment for cirrhotic patients with reduced sleep quality and QOL.
Factors Influencing Stress, Anxiety, and Depression Among Iranian Pregnant Women: The Role of Sexual Distress and Genital Self-image
Authors: Keramat Afsaneh , Malary Mina , Moosazadeh Mahmood , bagherian Nastaran , Rajabi-Shakib Mohammad-Reza 
Published: October 2020

Abstract
Background: Pregnancy is a unique period with the increased likelihood of psychological changes and emotional disturbances such as depression, anxiety, and stress. In this study, we investigated the factors influencing depression, anxiety, and stress in pregnancy and identify their associations with Sexual Distress (SD) and Genital Self-Image(GSI). Methods: This was a descriptive, correlational, cross-sectional study performed between September 2019 and January 2020. Overall, 295 pregnant women completed a demographics and obstetric information checklist, Depression Anxiety and Stress Scale-21 (DASS-21), Female Genital Self-Image Scale (FGSI), and Female Sexual Distress Scale-Revised (FSDS-R).Results: The mean score of SD was significantly associated with the severity of depression, anxiety, and stress. Also, the mean score of GSI was negatively correlated with the severity of depression and anxiety. Multiple linear regression analysis revealed that the risk factors for depression include SD, fear of fetal abortion, bad experience in previous pregnancy, poor GSI, and advancing age, whereas satisfaction with income was a protective factor. SD, fear of fetal abortion, and increased duration of marriage were found to be associated with the development of anxiety symptoms, whereas satisfaction with income, positive body image, and increased gestational age had a protective effect against this variable. SD, adverse events in previous pregnancies, and advancing age were the risk factors, while planned pregnancy and increased gestational age were the protective factors against developing stress during pregnancy. Conclusion: Many factors influence the development of depression, anxiety and stress in pregnancy, which may be different or common. Some of these factors are avoidable and some are not. Therefore, considering all the factors comprehensively with emphasis on the improvement of preventable factors by screening and counseling can be useful for mothers and their fetuses.
What was the Impact of a Global Pandemic (COVID-19) Lockdown Period on Experiences within an Eating Disorder Service? A Service Evaluation of the Views of Patients, Parents/Carers and Staff.
Authors: Shaw Hannah , Robertson Sarah , Ranceva Nadia 
Published: October 2020

Abstract
Background: The World Health Organization declared the outbreak of COVID-19 as a global pandemic on the 11th March 2020. As a result, the UK Government imposed severe restrictions on working and social contact as part of “lockdown.” Whilst the full extent of the pandemic’s impact on eating disorder patients is unknown, the literature suggests that patients with pre-existing mental illness may be more vulnerable to the mental health impacts. In addition, the restrictions greatly reduced the access to mental health services and presented new challenges to service delivery. A service evaluation was carried out to explore how the COVID-19 global pandemic changed service provision in a young person’s eating disorder service and how this affected patients, family and staff experiences. Methods: Quantitative data was collected in an online survey and qualitative data was collected in two formats: open ended answers as part of the online survey and open-ended focus groups, structured using narrative enquiry. The 43 participants consisted of 12 patients, 19 parents/carers, and 12 staff members. Patients were under the age of 18 and had a diagnosis of an eating disorder. Results: COVID-19 and lockdown increased the pressure on the service and changed service provision significantly. This has impacted the relational experiences for patients and their carers and staff have been faced with new challenges. Patients, parents/carers and staff all preferred face-to-face appointments over virtual options. There was no difference in service satisfaction before and during COVID-19. Conclusions: It is possible to provide a high-quality eating disorder service in lockdown restrictions. Providing face-to-face appointments at the beginning of treatment and including families in the planning should be prioritised. Staff support is crucial to be able to continue delivering high quality services. The key themes are identified, and clinical recommendations are made to guide service delivery.
Up-regulated Endonuclease Regnase-1 Suppresses Osteoarthritis by Forming Negative Feedback Loop of Catabolic Signaling in Chondrocytes
Authors: Yang Jeong-In , Chun Jang-Soo 
Published: October 2020

Abstract
Background: Ribonucleases (RNases) play central roles in the post-transcriptional regulation of mRNA stability. Our preliminary results revealed that the endonuclease Regnase-1 is specifically up-regulated in osteoarthritic chondrocytes. We herein explored the possible functions and regulatory mechanisms of Regnase-1 in a mouse model of osteoarthritis (OA).Methods: The expression and target genes of Regnase-1 were identified by microarray analysis in primary-culture mouse articular chondrocytes. Experimental OA in mice was induced by destabilization of the medial meniscus (DMM). The function of Regnase-1 in DMM-induced post-traumatic OA mice was examined by adenovirus-mediated overexpression or knockdown in knee joint tissues, and also by using Regnase-1 heterozygous knockout mice (Zc3h12a+/-). Results: Among the RNases, Regnase-1 was exclusively up-regulated in chondrocytes stimulated with OA-associated catabolic factors. Adenovirus-mediated overexpression or knockdown of Regnase-1 alone in joint tissues did not cause OA-like changes. However, overexpression of Regnase-1 in joint tissues significantly ameliorated DMM-induced post-traumatic OA cartilage destruction, whereas knockdown or genetic ablation of Regnase-1 exacerbated DMM-induced cartilage destruction. Mechanistic studies showed that Regnase-1 appears to suppress cartilage destruction by modulating the expression of matrix-degrading enzymes in chondrocytes. Conclusion: Our results collectively suggest that up-regulated Regnase-1 in OA chondrocytes may function as a chondro-protective effector molecule during OA pathogenesis by forming negative feedback loop of catabolic signaling such as expression of matrix-degrading enzymes in OA chondrocytes.
Can Brain Natriuretic Peptide Be Used as an Indicator of Over-infusion in Patients with Acute Sepsis? : A Pilot Study Evaluating Correlation Between Brain Natriuretic Peptide and Global End-diastolic Volume Index
Authors: Kishihara Yuki , Yasuda Hideto , Morisawa Kenichiro , Fujitani Shigeki , Homma Yosuke , Shigemitsu Kazuaki , Saito Nobuyuki , Hayakawa Katsura , Hifumi Toru , Rinka Hiroshi , Mayumi Toshihiko , Fujiwara Shinsuke , Murao Yoshinori , Taira Yasuhiko 
Published: October 2020

Abstract
Background: Recently, for patients with sepsis, under-infusion, that hastens the progression to multiple organ failure and death, has been avoided; however, the rate of over-infusion and resulting complications, such as mortality has increased. There are few indicators of over-infusion, such as transpulmonary thermodilution; therefore, minimally invasive, simple, and quick indicators of over-infusion are needed. This pilot study aimed to determine the association between brain natriuretic peptide (BNP) and global end-diastolic volume index (GEDI).Methods: This was a post-hoc analysis of a multicenter prospective randomized controlled study, performed in the intensive care units (ICU) of 10 separate hospitals in Japan from September 2013 to March 2016. Patients with sepsis who were expected to require mechanical ventilation for more than 48 hours were included. We measured BNP and GEDI every 24 hours from ICU admission until next 72 hours. The primary outcome was the correlation between BNP and GEDI that was assessed in two ways: 1) GEDI considered a continuous variable, and 2) GEDI divided into two groups based on a cutoff of 850 ml/m2, which is the upper limit of normal GEDI. We used a univariable generalized linear mixed-effects model in which patients are considered as a random effect.Results: Fifty-seven patients were analyzed. GEDI remained under the upper limit of normal GEDI (850 ml/m2) from ICU admission to 72 h, and there were no significant changes in the trends of BNP and GEDI over time. BNP tended to be higher in group 1 (GEDI >850 ml/m2) from ICU admission to 72 h, but there was no significant difference in the BNP values. The regression coefficient and 95%CI of BNP for GEDI were 2.5 and 1.3 to 3.7 when considered as a continuous variable, and 166 and -273 to 605 when divided into two groups based on GEDI cutoff of 850 ml/m2. Conclusion: There could be a weak but significant correlation between GEDI and BNP considering GEDI as a continuous variable. Using BNP as an indicator of over-infusion may be possible; however, further studies are needed.Trial registration: None.
γ-Cyclodextrin&nbsp;Metal-Organic Framework as a Carrier to Deliver Triptolide for the Treatment of Hepatocellular Carcinoma
Authors: Li Zhe , Yang Gang , Wang Rong , Wang Yuanyuan , Wang Jing , Yang Meng , Gong Chunai , Yuan Yongfang 
Published: October 2020

Abstract
Triptolide (TPL) has been employed to treat hepatocellular carcinoma (HCC). However, the poor water-solubility of TPL restrict its applications. Therefore, we prepared TPL loaded cyclodextrin-based metal-organic framework (TPL@CD-MOF) to improve the solubility and bioavailability of TPL, thus enhancing the anti-tumor effect on HCC. The BET surface and the pore size of TPL@CD-MOF were 1134.5 m2·g−1 and 1.6 nm, respectively. The results of XRD indicated that TPL in TPL@CD-MOF was encapsuled. TPL@CD-MOF showed a slower release than free TPL in vitro. Moreover, the CD-MOF improved the cell internalization and bioavailability of TPL. TPL@CD-MOF also showed higher anti-tumor efficacy in vitro and in vivo compared to free TPL. As a carrier, CD-MOF improved the solubility and bioavailability of TPL. In addition, TPL@CD-MOF exhibited improved anti-tumor effects in vitro and in vivo, indicating great potential as a carrier for insoluble anti-tumor drugs.
H19 contributes to aerobic glycolysis, proliferation and immune escape of gastric cancer cells via the miR-519d-3p/LDHA axis
Authors: Sun Linqing , Li Juntao , Yan Wenying , Yao Zhendong , Wang Ruoqin , Zhou Xiaojun , Wu Hongya , Zhang Guangbo , Shi Tongguo , Chen Weichang 
Published: October 2020

Abstract
Background Long non-coding RNAs (lncRNAs) have been investigated in multiple human cancers including gastric cancer (GC). Our research aims to explore the role of H19 in aerobic glycolysis, proliferation, and immune escape of GC cells.Methods The expression of H19 in GC samples were analyzed using Gene Expression Profiling Interactive Analysis (GEPIA), Gene Expression Omnibus (GEO) data, and real-time quantitative polymerase chain reaction (RT-qPCR) analysis. Glucose consumption and lactate production were applied to assess the aerobic glycolysis of GC cells. Subcellular fractionation, luciferase reporter, and western blot assays certified the binding between genes. CCK-8 and colony formation assays were used to determine GC cell proliferation. Flow cytometry, ELISA, and RT-qPCR assays were applied to analyze the immunosuppressive effect of H19. Results H19 was highly expressed in samples of patients with GC, and associated with tumor growth in vivo. H19 knockdown suppressed glucose consumption, lactate production, proliferation of GC cells by regulating miR-519d-3p/LDHA axis. Both miR-519d-3p depletion and LDHA overexpression could reverse the H19 knockdown-induced decrease in aerobic glycolysis and proliferation. Moreover, conditioned medium (CM) from stable knockdown H19 GC cells modulated the activity of immune cells including γδT cells, Jurkat cells, and tumor-associated macrophages (TAMs) in a lactate dependent manner.Conclusions The H19/miR-519d-3p/LDHA axis mainly contributed to aerobic glycolysis, proliferation, and immune escape of GC cells.
Circular RNA hsa_circ_0006401 promotes proliferation and metastasis in colorectal carcinoma
Authors: Zhang Chenjing , Zhou Xiaolu , Geng Xiaoge , Wang Jingya , Zhang Yu , Jing Jiyong , Pan Wensheng 
Published: October 2020

Abstract
Background: Dysregulation of circular RNA (circRNA) expression is involved in the progression of cancer. Here, we aimed to study the potential function of hsa_circ_0006401 in colorectal cancer (CRC).Methods: CircRNA hsa_circ_0006401 expression levels in CRC and adjacent nontumor tissues were analyzed by real-time quantitative PCR (qRT-PCR) and circRNA in situ hybridization (RNA-ISH). Two siRNAs were designed according to the splice junction of hsa_circ_0006401, and both effectively downregulated the hsa_circ_0006401 expression level. Then, CRC cell proliferation was assessed by cell counting. Wound-healing and transwell assays were utilized to detect the effect of hsa_circ_0006401 on CRC migration. A circRNA-ORF-GFP construct was created, and a specific antibody against the splice junction of hsa_circ_0006401 was prepared.Results: Hsa_circ_0006401 was closely related to CRC metastasis and exhibited upregulated expression in metastatic CRC tissue samples. Proliferation and migration were inhibited in vitro when hsa_circ_0006401 expression was silenced. Downregulation of hsa_circ_0006401 expression decreased CRC proliferation and liver metastasis in vivo. A 198-aa peptide was encoded by sequences of the splice junction absent from col6a3. Hsa_circ_0006401 promoted CRC proliferation and migration by encoding the hsa_circ_0006401 peptide. Hsa_circ_0006401 peptides decreased the protein level of the host gene col6a3 but did not alter its gene expression level.Conclusion: Our study revealed that circRNAs generated from col6a3 that contain an open reading frame (ORF) encode a novel 198-aa functional peptide and hsa_circ_0006401 peptides transcriptionally regulates the host gene col6a3 to promote CRC proliferation and metastasis.