bluuebunny/crossref_metadata_2025_split huggingface dataset:
Downregulation of m6A reader YTHDC2 promotes the proliferation and migration of lung cancer and cigarette smoke-exposed BEAS-2B cells
Authors: Wang Jin , Tan Lirong , Jia Beibei , Yu Xiaofan , Yao Ruixin , Ouyang Nan , Tong Jian , Chen Tao , Chen Rui , Li Jianxiang 
Published: December 2020

Abstract
Background: Lung cancer is one of the most common carcinomas worldwide. Cigarette smoking is considered as the leading cause of lung cancer. The aberrant expression of several YTH family proteins has been reported to be closely associated with several cancers. This study aims to evaluate the expression profile and function of YTHDC2, an RNA m6 A methylation reader protein, by in vitro and animal experiments and bioinformatics analysis. Methods: The expression of YTHDC2 gene and protein was analyzed by the GEPIA online tool and Gene Expression Omnibus (GEO) database, as well as immunohistochemistry of tissue arrays. The YTHDC2 expression in cigarette smoke-exposed cells was examined by RT-qPCR and western blot assays. The TCGA datasets and proteomic analysis of YTHDC2 knockdown cells were used to predict the clinical significance and biological function of YTHDC2. Functional assays, including wound healing, transwell migration, cell cycle and cell proliferation, were used to determine the biological role of YTHDC2 in lung cancer. The xenograft animal model further validated the tumor suppressor effect of YTHDC2 in vivo. The copy number variation of YTHDC2 was analyzed by TCGA datasets and TaqMan copy number assay. Results: YTHDC2 was significantly low-expressed in lung cancer and cigarette smoke-exposed cells. Notably, decreased YTHDC2 was found highly associated with smoking history, pathological stage, invasion depth, and lymph node metastasis, as well as poor outcomes. The YTHDC2 related genes and proteins were enriched in several cancer-related pathways, and several tumor suppressor genes were found positively correlated with YTHDC2. The in vivo and in vitro study revealed that overexpression of YTHDC2 inhibited the proliferation and migration ability of lung cancer cells, as well as the tumor growth in nude mice. Besides, a significant positive correlation between the copy number and expression level of YTHDC2 was found in lung cancer. Conclusions: Our results indicated that the smoking-related down-regulation of YTHDC2 was associated with enhanced proliferation and migration of lung cancer cells and appeared to be regulated by DNA copy number variation. Importantly, YTHDC2 may serve as a tumor suppressor gene and a favorable prognostic indicator in lung cancer patients.
WITHDRAWN: microRNA-4488 in extracellular vesicles derived from marrow mesenchymal stem cells suppresses ABHD8 to inhibit ovarian cancer progression
Authors: Chang Lei , Zhou Junying , Tian Wanjia , Chen Mengyu , Guo Ruixia , Lei Ningjing 
Published: December 2020

Abstract
Background
Extracellular vesicle (EV) that delivered microRNAs (miRNAs) have been found as the important biomarkers participating in the pathological mechanism of ovarian cancer. Consequently, this study sought to examine the underlying mechanism of mesenchymal stem cell (MSC)-derived EVs containing miR-4488 in ovarian cancer.
Methods
The normal ovarian tissues and ovarian cancer tissues were extracted, and the information of MSC-EV miRNA was obtained by Bioinformatics analysis. RT-qPCR and western blot analysis were applied to detect miR-4488 and α/β-hydrolase domain-containing (ABHD)8 expression followed by determination of relationship between miR-4488 and ABHD8 by dual-luciferase reporter assay. After transfection with different plasmids and treatment with DMSO or GW4869 (inhibitor of EV), the regulatory roles of MSC-EV-miR-4488 in invasion, proliferation, apoptosis, and migration of cancer cells were explored. Besides, xenograft tumor in nude mice was conducted to explore the role of miR-4488 and ABHD8 in ovarian cancer in vivo.
Results
miR-4488 was poorly expressed and ABHD8 was highly expressed in ovarian cancer cells and tissues. ABHD8 was a target gene of miR-4488 while the knockdown of ABHD8 resulted in the suppression of proliferation, invasion, and migration while promoting the apoptosis of cancer cells. Functionally, MSC-EV-derived miR-4488 inhibited the expression of ABHD8. Additionally, miR-4488 over-expressed in MSC-EVs inhibited the cell proliferation, invasion, and migration through down-regulation of ABHD8 expression. At last, these in vitro findings were also confirmed in vivo.
Conclusion
To summarize, miR-4488 overexpressed in MSC-EVs suppressed ABHD8 expression to inhibit the cancer cell proliferation, invasion, and migration, thus suppressing ovarian cancer.
Integrated analysis of multi-omics data to identification of prognostic genes for pancreatic cancer
Authors: Jiang Feng , Zhang Fan , Chen Jian , Wang Junjun , Hu Lijuan , Chen Jie , Wang Yumin 
Published: December 2020

Abstract
Background

We aim to develop core modules related to pancreatic cancer (PC) to predict the prognosis of PC patients and explore their tumor microenvironment.
Method:

We merged 175 pancreatic cancer samples in the TCGA database with gene mutation expression, methylation level distribution, mRNA expression and pancreatic cancer-related genes into a public database, and then through weighted correlation network analysis (WGCNA), Two expression modules associated with pancreatic cancer are combined. Then, by integrating these selected genes identified from the first 10 genes of the two co-expression modules, a model risk score is established, and patients are divided into high-risk and low-risk subgroups. Kaplan-Meier survival analysis method is used to analyze differences, analyze the correlation of survival between subgroups, and analyze prognostic models. These selected core genes can divide early pancreatic cancer into two subgroups, compare the prognosis of these two groups, and screen for differentially expressed genes. Use GO and KEGG enrichment analysis to predict the function of differentially expressed genes. The differential expression level and immune cell infiltration level of these selected core genes were further analysis.
Results

Our findings shown nine core genes (MST1R, TMPRSS4, PTK6, KLF5, CGN, ABHD17C, MUC1, CAPN8, B3GNT3) were prognostic biomarkers of pancreatic cancer. These 9 genes could divide early pancreatic cancer into two subgroups, and the two subgroups had significant differences in prognosis, and were mainly different in functions such as digestion and extracellular cell adhesion. Further analysis revealed that the expression of these 9 genes were expressed at high levels in pancreatic cancer tissues. In addition, we validated pancreatic cancer cells and pancreatic epithelial cells through quantitative real-time PCR (qRT-PCR), suggesting that the MST1R, PTK6, ABHD17C and CGN expressed higher in PC cells. CIBERSORT analysis indicated that these genes expression were closely correlated with B-cell naive, CD8+ T cells, Macrophages M0 cells, suggesting that these genes may play a carcinogenic role in the preservation of immune-dominant status for tumor microenvironment.
Conclusions

Our research identified 9 key genes which may enhance our understanding of the molecular mechanisms associated with pancreatic cancer.
APACHE IV is an accurate measure of predicting in-hospital mortality of ICU patients, but there are covariates associated with its occasional failure - a multicentre analysis of intensive care units
Authors: Feng Shuo , Dubin Joel A 
Published: December 2020

Abstract
Background: APACHE IV provides typically useful and accurate predictions on in-hospital mortality and length of stay for patients in critical care. However, there are factors which may preclude APACHE IV from reaching its ceiling of predictive accuracy. Our primary aim was to determine which variables available within the first 24 hours of a patient’s ICU stay may be indicative of the APACHE IV scoring system making occasional but potentially illuminating errors in predicting in-hospital mortality. Methods: We utilized the publicly available multi-institutional ICU database, eICU, available since 2018, to identify a large observational cohort for our investigation. APACHE IV scores are provided by eICU for each patient’s ICU stay. We used Lasso logistic regression in an aim to build parsimonious final models, using cross-validation to select the penalization parameter, separately for each of our two responses, i.e., errors, of interest, which are APACHE falsely predicting in-hospital death (Type I error), and APACHE falsely predicting in-hospital survival (Type II error). We then assessed the performance of the models with a random holdout validation sample. Results: While the extremeness of the APACHE prediction led to dependable predictions for preventing either type of error, there were a small set of distinct variables identified as being strongly associated with the two different types of errors occurring. These included worst lactate and mean SpO2 for Type I, and mean non-invasive blood pressure and mean respiratory rate for Type II. The two models also differed in their performance metrics in identical holdout validation samples, in large part due to the lower prevalence of Type II errors compared to Type I. Conclusions: The eICU database was a good resource for evaluating our objective, and important recommendations to intensivists are provided, particularly identifying key variables that could lead to APACHE prediction errors when APACHE scores are sufficiently low to predict in-hospital survival.
Efficacy of airway pressure release ventilation for acute respiratory distress syndrome: A meta-analysis
Authors: Junhai Zhen , Bangchuan Hu , Shijin Gong , Yihua Yu , Jing Yan , Li Li 
Published: December 2020

Abstract
Background

Airway pressure release ventilation (APRV) has been described many years, however, it is still unclear whether APRV improves outcomes in critically ill patients admitted to Intensive Care Unit with acute respiratory distress syndrome (ARDS).
Methods

3 databases were searched for randomized controlled trials (RCTs) until 8 August 2019. The relative risk (RR), mean difference (MD) and 95% confidence intervals (CI) were determined.
Results

A total of six randomized controlled trials (RCTs) were included with 360 ARDS patients. The Meta analysis showed that the mean arterial pressure (MAP) in APRV group is higher than traditional mechanical ventilation group [MD = 2.35, 95% CI=(1.05,3.64), P = 0.0004], and the airway peak pressure (Ppeak) is lower in APRV group with statistical difference [MD=-2.04,95% CI=(-3.33,-0.75), P = 0.002]. However, no significant beneficial effect on oxygen index (PaO2/FiO2) was shown between two groups (MD = 26.24, 95% CI=(-26.50,78.97), P = 0.33). Compared with conventional mechanical ventilation, APRV significantly improved 28-day mortality [RR = 0.66, 95% CI=(0.47,0.94), P = 0.02].
Conclusions

For critically ill patients with ARDS, application of APRV is associated with the increase of MAP, the reduction of the airway Ppeak and 28-day mortality, while there is no sufficient evidence to support the APRV is superior to conventional mechanical ventilation in PaO2/FiO2.
The Correlation Between Whole Blood Copper and Zinc Levels and Sepsis-Induced Left Ventricular Systolic Dysfunction (SILVSD) in Patients with Septic Shock: A Single-Center Prospective Observational Study
Authors: Meng Jian-Biao , Hu Ma-Hong , Zhang Wei , Hu Gong-Pai , Guo Xiao-Gang , Hu Shen-Jiang 
Published: December 2020

Abstract
Background: The dyshomeostasis of trace elements is associated with multiple organ dysfunction in patients with septic shock. However, it remains unclear whether the change of whole blood copper (Cu) and zinc (Zn) levels influence cardiac function in patients with septic shock. Here, we sought to explore relationship between whole blood Cu and Zn and cardiac dysfunction in septic shock. Methods: Between April 2018 and March 2020, septic shock patients with sepsis-induced left ventricular systolic dysfunction (SILVSD, left ventricular ejection fraction, LVEF ＜ 50%) and with no sepsis-induced myocardial dysfunction (non-SIMD, septic shock alone and LVEF＞ 50%) on an intensive care unit (ICU) in south China and healthy controls were prospectively enrolled. whole blood Cu and Zn levels were measured using flame atomic absorption spectrophotometry.Results: 86 patients with septic shock including 41 SILVSD and 45 non-SIMD and 25 healthy controls were studied. Whole blood Cu levels were significantly higher and Zn were significantly lower in SILVSD compared with non-SIMD and controls [Cu, (16.34±1.93) vs. (15.23±2.07) vs. (14.02±1.65) µmol/L, p = 0.009, < 0.001; Zn, (78.45±12.18) vs. (85.07±14.80) vs. (94.90±14.78) µmol/L, p = 0.029, < 0.001]. Both increased whole blood Cu and reduced Zn were associated with lower LVEF ( all p < 0.001) and higher amino-terminal pro-B-type natriuretic peptide (NT-proBNP) (Cu, p = 0.002, Zn, p = 0.001), and had predictive values for SILVSD [Cu, (AUC = 0.666; p = 0.005) ; Zn, (AUC = 0.625; p = 0.039)]. Whole blood Cu levels were increased but Zn were reduced in non-survivors compared with survivors[Cu, (17.20 ±2.25) vs. (14.99 ±1.49) µmol/L, p < 0.001; Zn, (71.17 ±11.98) vs. (87.67±11.30) µmol/L, p < 0.001],. Whole blood Cu and Zn displayed the value of predicting 28-day mortality [Cu (AUC = 0.802, p < 0.001); Zn (AUC = 0.869, p < 0.001)].Conclusions: Whole blood Cu levels were increased in SILVSD patients and positively correlated with the cardiac dysfunction while Whole blood Zn were reduced and negatively associated with the cardiac dysfunction. Moreover, both whole blood Cu and Zn could distinguish between SILVSD and non-SIMD in septic shock patients and predict 28-day mortality.Trial registration: ChiCTR1800015709. Registered 16 April 2018, http://www.chictr.org.cn/edit.aspx?pid=26746&htm=4.
Screening of Cardiac Surgery Patients - Polish Validation 4AT Tool
Authors: Krupa Sabina , Ozga Dorota , Tomaszek Lucyna , Tsevis Theofanis , Jurek Krzysztof , Religa Dorota , Maclullich Alasdair 
Published: December 2020

Abstract
IntroductionDelirium is a phenomenon which affects patients with various disorders and representing various age groups. Screening instruments make it possible to diagnose the condition at an early stage and to prevent its development. The article presents validation of the 4AT instrument, i.e. a two-minute test used to identify delirium.ObjectivesThe aim of the present study was to examine the reliability and validity of the Polish version of the 4AT (Pol4-AT) for use by nurses working in intensive care units.Patients and MethodsThe 4AT test was used to assess 444 patients at a cardiac surgery ward, on their sixth day in hospital, and the third day following a surgery. The patients were selected at random (in sequence any patient returning from a surgery and staying in hospital for six days). The conditions for entering the study included the subjects’ informed consent and age >18 years. ResultsThe current findings show that the 4AT scale can effectively be used in Poland, not only in geriatric patients but also at surgical wards and at ICUs. The study showed Cronbach’s alpha amounting to 0.843. ConclusionsThere is currently no validation tool in Poland that can be used in the diagnosis and screening of delirium. The CAM and CAM ICU scales, despite the fact that they have been translated into Polish, are not a standard used in practice in Poland
Gastrointestinal Ultrasonographic Findings In Cats With Feline Panleukopenia: A Case Series
Authors: Isaya Rosaria , Ciccarelli Stefano , Enache Daniela , Specchi Swan , Pesaresi Marco , Ferri Filippo , Porporato Federico , Auriemma Edoardo , Contiero Barbara , Coppola Luigi M , Zini Eric 
Published: December 2020

Abstract
Background-Feline panleukopenia virus (FPV) is very resistant and highly contagious and infects domestic cats and other felids. FPV is particularly widespread among sheltered cats, and is associated with high morbidity and mortality, causing severe gastroenteritis characterized by anorexia, lethargy, fever, dehydration, hemorrhagic diarrhea, and vomiting. There is currently no data on the ultrasonographic features of cats affected with FPV. This case series describes abdominal ultrasonographic findings in shelter cats with naturally-occurring FPV, and assesses whether are associated with clinical and laboratory findings. Cats affected by FPV were enrolled in the study if an abdominal ultrasound was performed within 12 hours of diagnosis. Clinical, laboratory and survival data were collected from medical records. Ultrasonographic examinations were reviewed for gastrointestinal abnormalities and their associations with the above data were explored.Results-Twenty-one cats were included. Nine cats (42.9%) died and 12 (57.1%) recovered. Based on ultrasonography, the duodenum and jejunum showed thinning of the mucosal layer in 70.6% and 66.6% of cats, thickening of the muscular layer in 52.9% and 57.1% of cats, and hyperechogenicity of the mucosa in 41.2% and 33.3%. Jejunal hyperechoic mucosal band paralleling the submucosa and irregular luminal surface were both observed in 33.3% of the cats. Survival was positively associated with increased jejunal mucosal echogenicity (P=0.003) and hyperechoic mucosal band (P=0.003). Peritoneal free fluid was positively associated with vomiting (P=0.002).Conclusion-This study provides ultrasonographic features of naturally-occurring FPV in cats, which, as expected, are compatible with gastroenteropathy. The most frequent findings were diffuse small intestine mucosal layer thinning, muscular layer thickening and mucosal hyperechogenicity, jejunal hyperechoic mucosal band and irregular luminal surface. Ultrasonographic features may be useful to complete the clinical picture and assess the severity of the gastroenteropathy in FPV cats. Prospective studies are needed to confirm ultrasonographic prognostic factors.
Management of chlamydia and gonorrhoea infections diagnosed in primary care using a centralised nurse-led telephone-based service: mixed methods evaluation
Authors: Horwood Jeremy , Brangan Emer , Manley Petra , Horner Paddy , Muir Peter , North Paul , MacLeod John 
Published: December 2020

Abstract
Background: Up to 18% of genital Chlamydia infections and 9% of Gonorrhoea infections in England are diagnosed in Primary Care. Evidence suggests that a substantial proportion of these cases are not managed appropriately in line with national guidelines. With the increase in sexually transmitted infections and the emergence of antimicrobial resistance, their timely and appropriate treatment is a priority. We investigated feasibility and acceptability of extending the National Chlamydia Screening Programme’s centralised, nurse-led, telephone management (NLTM) as an option for management of all cases of chlamydia and gonorrhoea diagnosed in Primary Care. Methods: Randomised feasibility trial in 11 practices in Bristol with nested qualitative study. In intervention practices patients and health care providers (HCPs) had the option of choosing NLTM or usual care for all patients tested for Chlamydia and Gonorrhoea. In control practices patients received usual care.Results: 1154 Chlamydia/gonorrhoea tests took place during the 6-month study, with a chlamydia positivity rate of 2.6% and gonorrhoea positivity rate of 0.8%. The NLTM managed 335 patients. Interviews were conducted with sixteen HCPs (11 GPs, 5 nurses) and 12 patients (8 female). HCPs were positive about the NLTM, welcomed the partner notification service, though requested more timely feedback on the management of their patients. Explaining the NLTM to patients didn’t negatively impact on consultations. Patients found the NLTM acceptable, more convenient and provided greater anonymity than usual care. Patients appreciated getting a text message regarding a negative result and valued talking to a sexual health specialist about positive results.Conclusion: Extension of this established NLTM intervention to a greater proportion of patients was both feasible and acceptable to both patients and HCP, could provide a better service for patients, whilst decreasing primacy care workload. The study provides evidence to support the wider implementation of this NLTM approach to managing chlamydia and gonorrhoea diagnosed in primary care.
Quality of life, social support and self-efficacy in women after a miscarriage
Authors: Iwanowicz-Palus Grażyna , Mróz Mariola , Bień Agnieszka 
Published: December 2020

Abstract
Background Pregnancy loss is typically experienced as a traumatic, critical event, which may lead to secondary psychological health disorders. Its burden involves both the experience of loss and related medical issues, which are associated with pain, hospitalization, limitation in one’s social roles, decreased sense of security, and changes in one’s perceived quality of life. The purpose of the present study was to evaluate levels of quality of life (QoL), social support and self-efficacy among women who had suffered a miscarriage.Methods The study was performed using a diagnostic survey method with questionnaires administered to 610 patients hospitalized due to spontaneous pregnancy loss in hospitals in Lublin (Poland). The instruments used were: the Berlin Social Support Scales (BSSS), the Generalized Self-Efficacy Scale (GSES), the WHOQoL–BREF questionnaire, and a standardized interview questionnaire.Results Respondents rated their overall quality of life (3.90 points) higher than their overall perceived health (3.66). In terms of social support, the highest scores were noted for perceived available instrumental support (M = 3.78), perceived available emotional support (M = 3.68) and actually received support (M = 3.60). The mean generalized self-efficacy score among the women after pregnancy loss was 30.29. Respondents’ QoL was significantly correlated with multiple social support subscales and self-efficacy (p < 0.05). Conclusions Women after a miscarriage perceive their overall quality of life as better than their overall health, while reporting the poorest QoL in the psychological domain. They also have a high level of self-efficacy. Regarding the types of social support, perceived available support, both instrumental and emotional, and actually received support was rated highly. Social support and self-efficacy contributed to better perceived QoL among the respondents.