bluuebunny/crossref_metadata_2025_split huggingface dataset:
Recognizing Proteins with Binding Function in Elymus nutans Based on Machine Learning Methods
Authors: Yang Zhe , Wang Juan , Yang Jia , Qi Zhi , He Jiahao 
Published: March 2020

Background:
We research the binding function proteins in Elymus nutans. Recognition
for proteins is essential for study of biology. Machine learning methods have been widely used for
the prediction of proteins.

Methods:
We used BLAST software for the function annotations of Elymus nutans. Besides, we used
machine learning methods to recognize proteins which are not annotated by the software. In the
process, we focused on identifying the proteins with binding functions. In our research, features are
extracted by four algorithms, and then selected by mutual information estimator. Here three classifiers
are constructed based on K-nearest neighbour algorithm and gradient boosting algorithm.

Results and Conclusion:
Experimental results show that there are 848 proteins with ATP binding
function, 113 proteins with heme binding function, 315 proteins with zinc-ion binding function,
135 proteins with GTP binding function and 21 proteins with ADP binding function. Furthermore,
we have successfully predicted the functions of 10 special protein sequences whose function
annotations cannot be obtained by making sequence alignment with seven famous protein databases.
Among them, seven sequences have ATP binding functions, one sequence has heme binding function,
one sequence has zinc-ion binding function and the other one has GTP binding function.
In Vitro Characteristics of Glioma Cells Targeting by OX26-modified Liposomal Cisplatin
Authors: Ashrafzadeh Maryam Sadat , Heydarinasab Amir , Akbarzadeh Azim , Ardjmand Mehdi 
Published: March 2020

Background:
Drug delivery to the brain tumor is limited due to the presence of the
blood-brain barrier (BBB).

Objective:
This study aimed to evaluate the therapeutic effects of cisplatin-loaded PEGylated liposomes,
targeted with the OX26 antibody (targeted liposomal cisplatin) against transferrin receptor
expressing rat glioma C6 cells in vitro.

Method:
The liposomes were synthesized using reverse phase evaporation method and were conjugated
to the OX26 monoclonal antibody. They were characterized in terms of size, drug encapsulation
efficiency, morphology and drug release experiments using dynamic light scattering, atomic
absorption spectrometry, scanning electron microscopy, and dialysis membrane methods. Then,
their biological activities were evaluated on targeting the BBB.

Results:
The characterization results showed that spherical nanodrug with a size of
157 nm and drug loading efficiency of 24% was synthesized, which released 64% of the loaded cisplatin
after 72 h in a controlled release manner. The nanoparticles caused an increase in the cisplatin
cytotoxicity effects by 1.7-, 1.8- and 1.8-fold, compared to cisplatin-loaded PEGylated liposomes
(liposomal cisplatin) after 24, 48 and 72h incubation, respectively against C6 cells. Moreover, targeted
liposomal cisplatin showed promising results in the transport of cisplatin across the BBB, in
which it caused an increase in the cisplatin cytotoxicity on C6 cells by 2.7- and 2.4-fold, compared
to cisplatin and liposomal cisplatin, respectively.

Conclusion:
Regarding the properties of the targeted liposomal cisplatin, it suggests that the potency
of the formulation, to be evaluated, for the transport of cisplatin across the BBB, delivers it to the
brain tumor in vivo.
Experimental Phage Therapies in Companion Animals with A Historical Review
Authors: Pyzik Ewelina , Radzki Radosław P. , Urban-Chmiel Renata 
Published: March 2020

Background:
The aim of the review was to comprehensively characterize the antimicrobial
efficacy of bacteriophages in eliminating pathogens occurring in companion animals, as an
alternative to antibiotics for controlling infections that pose potential threats to the health and life of
people and to the environment.

Methods:
The review contains detailed information on the characteristics and classification of bacteriophages
and an analysis of their life cycle. The dominant element is a detailed analysis of the
experimental use of bacteriophages in combating infections caused by various microorganisms in
companion animals with regard to their potential use in therapy.

Results:
It seems that in the near future, phage therapies will provide an alternative to antibiotics in
the treatment of diseases caused by multi-drug resistant bacteria in people and animals.

Conclusion:
The effectiveness of phage therapies depends on many factors and the properties of
the bacteriophages themselves, which requires comprehensive knowledge of them.
Ralstonia Mannitolilytica, an Unusual Pathogen in the Neonatal Intensive Care Unit: A Case of Neonatal Sepsis and Literature Review
Authors: Lampropoulos Panagiotis , Gkentzi Despoina , Tzifas Sotirios , Kapnisi Georgia , Karatza Ageliki , Kolonitsiou Fevronia , Dimitriou Gabriel 
Published: March 2020

Background:
Premature infants are considered high-risk subgroup for neonatal sepsis
due to yet defective immune system, interventions practised and synergy of factors favoring multiple
resistance of Gram-positive and Gram-negative pathogens to antimicrobial agents.

Case presentation:
We present a case of late-onset neonatal sepsis in a premature infant caused by
an uncommon pathogen; a premature infant of extremely low birth weight had in his 4th week of
life severe clinical deterioration with lethargy, fever, pallor, mottling, abdominal distention, tachycardia,
and worsening respiratory impairment. Full septic screen was performed, broad-spectrum
antibiotic therapy was initiated and supportive care per needs was provided. Blood cultures (endotracheal
tube tip cultures) isolated meropenem- and gentamicin-resistant strain of rare pathogen Ralstonia
mannitolilytica. Ralstonia spp. are aerobic, Gram-negative, lactose non-fermenting, oxidaseand
catalase-positive bacilli, thriving in water and soil. Ralstonia spp. are identified only sporadically
as causative agents of neonatal sepsis; to our knowledge, this is the second report of neonatal
sepsis due to R. mannitolilytica in the literature so far. Our patient was eventually treated (per sensitivity
pattern) with intravenous ciprofloxacin and recovered well from the infection.

Conclusion:
We intend to raise awareness among neonatologists with regard to early detection of
unusual pathogens, the emergence of antibiotic resistance patterns, and the obligation for adherence
to infection control policies.
Kinematic Differences between Two Types of Forward Elevations of the Shoulder Joint: Flexion and Reaching Elevation
Authors: Sahara Ryo , Hamada Junichiro , Yoshizaki Kunio , Endo Kazuhiro , Segawa Daisuke , Yamaguchi Mitsukuni 
Published: March 2020

Background:
Extension of the elbow joint is maintained during shoulder flexion. In contrast, the arm starts from the flexed position of the elbow joint and the joint gradually extends during reaching elevation.

Objectives:
This study aimed to compare the kinematic elements and electromyographic (EMG) activities of the rotator cuff muscles between flexion and reaching elevation.

Methods:
The study included 10 healthy young men. (average age, 21.5 ± 3.4 years), and measurements were performed on their dominant arms. A three-dimensional motion analyzer was used to record the following elements during shoulder flexion and reaching elevation: the angles of glenohumeral joint elevation and scapular upward rotation, scapulohumeral rhythm, external rotation of the humerus, and glenohumeral plane shifting from the coronal plane. The EMG activities in the supraspinatus, infraspinatus, subscapularis, and teres minor were recorded simultaneously.

Results:
The plane of reaching elevation was retained at 60° from the coronal plane. The glenohumeral planes (P < 0 .01) and the external rotation angles of the humerus below 90° of elevation (P < 0.05) were significantly different between both the motions. The EMG activities in the supraspinatus (P < .01), infraspinatus (P < 0.05), and teres minor (P < 0.01) were significantly lower while reaching elevation than those during flexion.

Conclusion:
The motion plane at 60° from the coronal plane, movement of the humeral external rotation, and EMG activities of the rotator cuff muscles were different during reaching elevation and shoulder flexion.
Physicochemical and Biological Aspects of Curcumin: A Review
Authors: Kriplani Priyanka , Guarve Kumar 
Published: March 2020

Background:
Curcuma longa L. is worldwide investigated as the well known indian spice
by virtue of its principal constituent, curcumin.

Objective:
The objective of this review is to study the biosynthetic pathway, extraction process,
chemistry, various analytical methods for identification of curcumin and its effect on biological system.

Methods:
Current prose and papers emphasizing the biosynthesis, extraction methods, analytical
methods and biological activities of curcumin have been identified and reviewed with particular
emphasis on their scientific impact and novelty.

Result:
Curcumin, principal constituent extracted from dried rhizomes of Curcuma longa L. is a hydrophobic
polyphenol meant to cure chronic malignancies like rheumatoid arthritis, Alzheimer’s, Inflammatory
bowel disease and many common ailments related to colon, lung, stomach and skin.

Conclusion:
This present review gives erudition of curcumin which paves way for further work.
OVARIAN INFERTILITY FACTOR IN PATIENTS OF LATE REPRODUCTIVE AGE
Authors: Uryupina K. V. , Kutsenko I. I. , Kravtsova E. I. , Gavryuchenko P. A. 
Published: March 2020

Objective: in women over 35 years of age, there is a progressive and age-related decline in fertility, which is due to multiple consequences, including a decrease in follicular reserve. Cytokines play a role, mediating the interaction between oocytes and other cells. In addition, there is a change in the expression of mRNA of a number of genes, leading to a decrease in the ability to bear children. The goal is to highlight the problem of reducing fertility in women of older reproductive age. Materials and methods: medLine, Pubmed, RISC, etc. Results: analysis of literature data shows that fertility decline is determined by a combination of physiological, molecular and genetic factors that play an increasing role as we age.Conclusion: the development of modern technology can solve the problem of infertility in the vast majority of cases. However, the lack of effectiveness of assisted reproductive technologies (ART) for women over 35 years of age requires optimizing a care strategy for these women.
EPISIOTOMY AS ONE OF THE PROBLEMS OF MODERN PERINIOLOGY
Authors: Berezhanskaya S. B. , Vostrykh N. N. , Golota A. N. , Sozaeva D. I. , Krynochkina M. Y. , Loginova I. G. 
Published: March 2020

Objective:the study is to determine the effectiveness of EEG-biofeedback training on β-rhythm for correcting voluntary attention in children 6 – 8 years of age with the consequences of perinatal CNS lesions of hypoxic-ischemic genesis.Materials and methods:a total of 120 children aged 6 – 8 years who included 30 children with cerebrastenic syndrome (I group) were examined; 58 children with hyperactivity and attention deficit syndrome (II group); 32 children with cerebral palsy, spastic diplegia (III group). EEG-biofeedback training on β-rhythm correction was carried out in an amount of 15 sessions lasting 20 – 30 minutes on the Kinesis machine (Neurot, Russia). Before and after the biofeedback therapy, a psychodiagnostic evaluation of the functions of voluntary attention on the Toulouse-Pieron scale was carried out (in the adaptation of LA Yasyukova, 2001). EEG recording was performed on an EEG 21/26 encephalograph Encephalan-131-03, modification 10 (Manufacturer: NPKF Medikom MTD, Russia). For statistical analysis of the obtained indicators before and after treatment, the significance criterion was used to compare the two proportions (fractions) under the assumption that the variables are distributed according to the normal law (package STATISTICS 6.0).Results:the effectiveness of EEG-biofeedback training on β-rhythm correction was revealed, as evidenced by a significant decrease in the number of children with isolated types of attention disorders in all groups of patients as well as with mixed disorders in the II group.Conclusion:аctivation of cortical processes, increasing the level of attention, memory and cognitive abilities of the psyche, when EEG-biofeedback training is included in the therapy complex, depend on the nature and severity of central nervous system lesions in children with various nosological forms of neurological pathology, which requires rethinking of therapeutic complex methods of correction and selection of personalized biofeedback training protocols, taking into account data on various types of metabolic and neurophysiological abnormalities in the head in children with different nosological forms of neurological disorders.
ISTHMIC-CERVICAL INSUFFICIENCY IN THE STRUCTURE OF THE REASONS FOR MORTARING OF PREGNANCY
Authors: Borschova A. A. , Pertceva G M. , Alekseeva N. A. 
Published: March 2020

Objective: to identify the main causes that contribute to the occurrence of ischemic-cervical insufficiency.Materials and methods: a retrospective analysis of the course of pregnancy, childbirth, outcomes for the fetus in 158 women with ischemic-cervical insufficiency was performed. All pregnant women, except for general clinical examination, underwent transvaginal ultrasound examination of the cervix.Results: of 158 women with isthmic-cervical insufficiency, there were 71 first-born (44.94 %), and 87 second-born (55.06 %). In the history of these women, 64 (40.51 %) had inflammatory processes of the female genitals, 38 (24.05 %) had extragenital pathology (cardiovascular diseases), 36 (22.78 %) were obese, 13 (8.23 %) had cervical erosion, and only 7 (4.43 %) had a favorable history. The diagnosis of ICN was established by a combination of data from vaginal and transvaginal ultrasound examination of the cervix. Correction of this pathology was carried out by applying a pessary to the cervix. Conclusion: we did not find a statistically significant difference between the occurrence, causes, and manifestation of clinical data in both first-and second-generation women. Among the causes of PPI, both in the first and second clinical groups, inflammatory diseases of the female genitals, cardiovascular pathology and obesity predominate. Early diagnosis of PPI reduces the risk of premature birth, reduces the percentage of premature birth, and contributes to the birth of children during full-term pregnancy.
FEATURES OF THE COURSE OF PREGNANCY, CHILDBIRTH AND POSTPARTUM PERIOD IN PATIENTS WITH ADENOMYOSIS
Authors: Kravtsova E. I. , Kutsenko I. I. , Avakimyan A. A. 
Published: March 2020

Objective: to assess the course of pregnancy, childbirth and postpartum period in patients with adenomyosis 1 and 2 degrees.Materials and methods: the course of pregnancy and its outcomes were analyzed in 153 primordial women with a history of adenomyosis of 1–2 degrees (I group), the control group (II group) — 150 conditionally healthy primordial women. Results: in preterm patients with adenomyosis of 1 and 2 degrees, in the absence of problems with conception and somatic pathology, statistically significantly increases the number of early reproductive losses and premature birth during pregnancy statistically significantly more often formed placental insufficiency and increases the frequency of hypertension disorders, increases the frequency of pathology of labor and postpartum period, mainly associated with increased blood loss. Conclusion: complications of gestation, pathology of labor and postpartum period in patients even with 1 stage of adenomyosis are largely associated with histological, immunohistochemical and immunological features of the structure of the transition zone «endometrium-myometrium». Pathological processes occurring in the zone of remodeling of spiral arterioles may lead to abnormal chorion formation, which causes most obstetric complications.
THORACO-CARDIAC PARAMETERS IN CHILDREN WITH DIFFERENT VARIANTS OF SOMATIC DEVELOPMENT ACCORDING TO ULTRASOUND DATA
Authors: Lebedenko A. A. , Tarakanova T. D. , Semernik O. E. , Appoeva A. A. , Ivanova D. N. 
Published: March 2020

Objective: to study the ultrasound characteristics of the structure and location of the heart and blood vessels in children of different age groups, taking into account the harmony of physical development.Materials and methods: 160 people aged 4 to 18 years were surveyed. All the children included in the study were divided into four groups, according to the WHO. Anthropometric dimensions were evaluated using centile tables. There are 3 rates of physical development: age-appropriate; advancing age; lagging behind age norms, as well as harmonious, moderately disharmonious and high disharmonious development. Echocardiography was performed on a VIVID-3 scanner, General Electric, USA, using sector sensors with a frequency of 3-8 MHz. Statistical processing of the results were performed using the Microsoft Office Excel 2003 and Statistica 12.0 for Windows software package. Results: the morphometric parameters of cardial structures increase proportionally with age, having a significant range of deviation in the group. Disharmonious type of physical development is most often found in children of puberty. Among children of age I and II groups, harmonious physical development dominates and, in a small number, a moderately disharmonious type is formed-5 (14.3 %) and 11 (20.3 %) cases, respectively. Conclusion: the pronounced inter-group differences between the studied generally accepted and additional ultrasound dimensions of the heart testify to the expediency of using them as additional measurements in the experimental modeling of the chest cavity.
THE EFFECT OF LIGHT DEPRIVATION ON FUNCTIONAL PROCESSES IN THE SYSTEM “MOTHER – PLACENTA – FETUS” IN CASE OF THREATENING PRETERM BIRTH AND ITS CLINICAL SIGNIFICANCE
Authors: Pelipenko I. G. , Botasheva T. L. , Lebedenko E. Yu. , Mikhelson A. F. , Zheleznyakova E. V. , Zavodnov O. P. 
Published: March 2020

Objective: to study the nature of uterine contractile activity, hormonal status, melatonin metabolism, blood flow in the uterine and fetal vessels, and the state of the fetus wellbeing before and after standard drug treatment and combination therapy involving the additional use of light deprivation. Materials and methods: a total of 547 women with threatening preterm birth were examined at 28 – 35 weeks of pregnancy. Among these, 198 pregnant women received standard therapy and 196 women received combined treatment, medical along with light deprivation. The control group consisted of 153 women with physiological pregnancy. We used cardiotocography to study the contractile activity of the uterus and the fetal cardiac rhythm, enzyme-linked immunosorbent assay methods to study the expression of hormones (ACTH, cortisol, progesterone, estriol, placental lactogen) in the blood and 6-sulfatoxymelatonin in the urine of pregnant women. Results: threatening preterm labor is accompanied by an increase in the level of generalized (bilateral) uterine activity, a decrease in the level of melatonin and an increase in the level of stress hormones. After using standard drug therapy, there is a suppression of any form of uterine activity, which leads to a deterioration of the fetus state in 30,2% of women, while combined therapy increases the level of melatonin, decreases the activity of stress-liberating subsystems of the mother’s body, and increases the level of right-sided uterine contractions that contribute to maintaining functional activity of the uteroplacental “pump”, contributing to the prevention of fetal distress. Conclusions: the studies indicate an improvement in the therapeutic effect with using light deprivation, as an additional method for tocolysis in women with the threat of premature birth.
THE RELATIONSHIP BETWEEN THE EFFECT OF IMMUNOGLOBULIN THERAPY AND THE FUNCTIONAL POTENCIES OF THE CELLULAR LINK OF THE IMMUNE SYSTEM IN X-LINKED AGAMMAGLOBULINEMIA
Authors: Sizyakina L. P. , Andreeva I. I. , Danilova D. I. 
Published: March 2020

Objective:to evaluate the effectiveness of intravenous immunoglobulins in X-linked agammaglobulinemia, to identify changes in the cellular link of the innate and adaptive immune response associated with an insufficient effect of replacement therapy.Materials and methods:12 patients with X-AGH were under dynamic observation. BTK genetic defect confirmed using next generation sequencing technology. After verification of the diagnosis, patients received IVIG replacement therapy at a saturation dose and then at a maintenance dose. The data of prospective observation for a full year of regular IVIG therapy with an assessment of the severity of the disease, monthly monitoring of the pre-transfusion IgG level and determination of the quantitative and functional parameters of the components of the adaptive and innate immune response every three months are presented.Results:regular replacement therapy contributed to a decrease in the frequency of exacerbations of foci of chronic infection, a decrease in the frequency and duration of antibiotic therapy, however, in 30 % of cases, the frequency of exacerbations remained high, and antibiotic courses were doubled than the standard ones. A comparative analysis of the parameters of the immune system showed that with a smaller effect of IVIG, the functional potentials of T-effectors are less significant than in the comparison group and the properties of neutrophils and natural killers are more inhibited.Summary:the relationship between the severity of the clinical manifestation of XLA on the background of replacement therapy and the preservation of the functional potencies of the cellular components of the immune system provides the basis for a possible combination of IVIG therapy with drugs that affect the properties of cellular elements of the immune system.