bluuebunny/crossref_metadata_2025_split huggingface dataset:
Zinc interference with Cd‐induced hormetic effect in differentiated Caco‐2 cells: Evidence for inhibition downstream ERK activation
Authors: Lemaire Joannie , Mireault Myriam , Jumarie Catherine 
Published: December 2019

AbstractCadmium (Cd) is a toxic metal that enters the food chain. Following oral ingestion, the intestinal epithelium represents an effective protective barrier against Cd toxicity, but it is also a target tissue that may accumulate and trap high levels of the ingested metal. Using human enterocytic‐like Caco‐2 cells, we have previously shown that Cd may induce a concentration and time‐dependent increase in 3‐(4,5‐dimethyl‐2‐thiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide assay (MTT)‐reducing activity in differentiated cultures with correlation to ERK1/2 activation. The present study shows that (a) Zn prevents the Cd‐induced hormesis effect on MTT reduction in a concentration‐dependent manner, without inhibiting Cd‐induced ERK1/2 activation; (b) Zn also induces similar hormetic stimulation of MTT‐reducing activity but without ERK1/2 activation. The effect of both metals was sensitive to inhibitors of translation during protein synthesis. There is evidence for the involvement of reactive oxygen species (ROS) in Cd‐induced ERK1/2 activation. In contrast, the Zn effect on the MTT‐reducing activity would not be triggered by ROS but it would be sensitive to the redox state of the cell. Steps downstream ERK1/2 activation by Cd does not involve eIF4E which is rather downregulated by Cd. In conclusion, Cd and Zn both can modify translation processes during protein synthesis via different signaling cascades with crosstalk, and cross‐inhibition may occur. This phenomenon is observed over a small range of metal concentrations and is characterized by a hormesis‐like response. Considering that the hormetic effect on dehydrogenase activity could reflect an adaptive response to the metals whether cross‐inhibition is beneficial is an open question.
Data mining analysis of the PP2A cell cycle axis in mesothelioma patients
Authors: Pippa Raffaella , Boffo Silvia , Odero Maria D. , Giordano Antonio 
Published: December 2019

AbstractMesothelioma is an aggressive tumor that affects thousands of people every year. The therapeutic options for patients are limited; hence, a better understanding of mesothelioma biology is crucial to improve patient survival. To find new molecular targets and therapeutic strategies related to the protein phosphatase 2A (PP2A) network, we analyzed the gene expression of known PP2A inhibitors in mesothelioma patient samples. Our analysis disclosed a general overexpression of all PP2A‐negative regulators in mesothelioma patients. Moreover, the expression of ANP32E and CIP2A genes, increased in 16% and 11% of cases, positively correlates with the ones of all the other PP2A regulators and the ones of the main cyclins and CDKs, suggesting the existence of a feed‐forward loop that might contribute to the mesothelioma progression via PP2A inactivation. Overall, our study indicates the existence of a strategic and targetable axis between PP2A inhibitors (ANP32E and CIP2A) and cell cycle regulators (cyclin B2/CDK1) and provides a valuable rationale for using a personalized combinational therapy approach to improve mesothelioma patient survival.
Fetal mediastinal teratoma: Misinterpretation as congenital cystic lesions of the lung on prenatal ultrasound
Authors: Darouich Sihem , Bellamine Houda 
Published: December 2019

AbstractCongenital mediastinal teratoma can lead to development of hydrops fetalis and may be misinterpreted on ultrasound. In this case report, ultrasound revealed severe fetoplacental hydrops, moderate posthemorrhagic hydrocephalus, and multiple pulmonary cysts suggesting cystic adenomatoid malformation and displacement of the heart to the left side. Autopsy of the hydropic 24‐weeks male fetus showed a large cystic‐solid mediastinal mass that was consistent with nonmetastatic immature teratoma. It also demonstrated thymic, cardiac and pulmonary hypoplasia, and confirmed the germinal matrix‐intraventricular hemorrhage. Accurate prenatal diagnosis of mediastinal teratoma may be achieved by a careful Doppler ultrasound assessment that also allows evaluating the fetal outcome.
Expression, immune infiltration and clinical significance of SPAG5 in hepatocellular carcinoma: A gene expression‐based study
Authors: Chen Wei , Chen Xing , Li Sijin , Ren Biqiong 
Published: December 2019

AbstractBackgroundOverexpression of sperm‐associated antigen 5 (SPAG5) is a marker of poor prognosis in numerous tumors and is recognized as an index of tumor proliferation; however, its expression in liver cancer remains unclear.MethodsThe Oncomine (https://www.oncomine.org) and Timer (https://cistrome.shinyapps.io/timer) databases were used to analyze the expression of SPAG5 in liver hepatocellular carcinoma (HCC) and normal liver tissues. The relationship between the expression of SPAG5 and immune infiltration of HCC was investigated using the Timer and GEPIA (http://gepia.cancer‐pku.cn) databases, and the mechanism was analyzed using Gene Set Enrichment Analysis. A Kaplan–Meier Plotter (http://kmplot.com/analysis) was used to evaluate the effect of SPAG5 on the prognosis of patients with HCC.ResultsThe results revealed that the SPAG5 expression level was positively correlated with the infiltration levels of CD8+ T cells, macrophages, neutrophils, and especially B cells and dendritic cells. In addition, SPAG5 expression was significantly associated with T cell exhaustion. The overall survival time, progression‐free survival time, recurrence‐free survival time and disease‐specific survival time were significantly reduced for HCC patients with high SPAG5 expression (p < 0.01) and high expression of SPAG5 was significantly associated with a poor overall survival time and progression‐free survival time of grade and stage II–III HCC (p < 0.05) but not with stage I HCC (p > 0.05). Additionally, the expression of SPAG5 is related to the p53 and cell cycle signal pathways.ConclusionsIn conclusion, SPAG5 is not only a marker of immune infiltration and poor prognosis, but also a potential therapeutic target for liver cancer.
Randomized controlled trial of a positive affect intervention to reduce HIV viral load among sexual minority men who use methamphetamine
Authors: Carrico Adam W , Neilands Torsten B , Dilworth Samantha E , Evans Jennifer L , Gόmez Walter , Jain Jennifer P , Gandhi Monica , Shoptaw Steven , Horvath Keith J , Coffin Lara , Discepola Michael V , Andrews Rick , Woods William J , Feaster Daniel J , Moskowitz Judith T 
Published: December 2019

AbstractIntroductionIn the era of HIV treatment as prevention (TasP), evidence‐based interventions that optimize viral suppression among people who use stimulants such as methamphetamine are needed to improve health outcomes and reduce onward transmission risk. We tested the efficacy of positive affect intervention delivered during community‐based contingency management (CM) for reducing viral load in sexual minority men living with HIV who use methamphetamine.MethodsConducted in San Francisco, this Phase II randomized controlled trial tested the efficacy of a positive affect intervention for boosting and extending the effectiveness of community‐based CM for stimulant abstinence to achieve more durable reductions in HIV viral load. From 2013 to 2017, 110 sexual minority men living with HIV who had biologically confirmed, recent methamphetamine use were randomized to receive a positive affect intervention (n = 55) or attention‐control condition (n = 55). All individual positive affect intervention and attention‐control sessions were delivered during three months of community‐based CM where participants received financial incentives for stimulant abstinence. The 5‐session positive affect intervention was designed to provide skills for managing stimulant withdrawal symptoms as well as sensitize individuals to natural sources of reward. The attention‐control condition consisted of neutral writing exercises and self‐report measures.ResultsMen randomized to the positive affect intervention displayed significantly lower log10 HIV viral load at six, twelve and fifteen months compared to those in the attention‐control condition. Men in the positive affect intervention also had significantly lower risk of at least one unsuppressed HIV RNA (≥200 copies/mL) over the 15‐month follow‐up. There were concurrent, statistically significant intervention‐related increases in positive affect as well as decreases in the self‐reported frequency of stimulant use at six and twelve months.ConclusionsDelivering a positive affect intervention during community‐based CM with sexual minority men who use methamphetamine achieved durable and clinically meaningful reductions in HIV viral load that were paralleled by increases in positive affect and decreases in stimulant use. Further clinical research is needed to determine the effectiveness of integrative, behavioural interventions for optimizing the clinical and public health benefits of TasP in sexual minority men who use stimulants such as methamphetamine.
Ensemble Learning for Early‐Response Prediction of Antidepressant Treatment in Major Depressive Disorder
Authors: Pei Cong , Sun Yurong , Zhu Jinlong , Wang Xinyi , Zhang Yujie , Zhang Shuqiang , Yao Zhijian , Lu Qing 
Published: December 2019

BackgroundIn order to reduce unsuccessful treatment trials for depression, neuroimaging and genetic information can be considered as biomarkers. Together with machine‐learning methods, prediction models have proved to be valuable for baseline prediction.PurposeTo propose an ensemble learning modeling framework that integrates imaging and genetic information for individualized baseline prediction of early‐stage treatment response of antidepressants in major depressive disorder (MDD).Study TypeProspective.SubjectsIn all, 98 inpatients with MDD.Field Strength/Sequence3.0T MRI and gradient‐echo echo‐planar imaging sequence.AssessmentParticipants were divided into responders and nonresponders based on reducing rates of HDRS‐6 after early‐stage treatment of 2 weeks. Fourteen brain regions of interest were selected according to previous studies. An ensemble learning modeling framework was used to integrate imaging data and genetic data.Statistical TestsSupport vector machine (SVM) with linear kernel was utilized to integrate multimode information and then to construct the prediction model. Leave‐one‐out cross‐validation (LOOCV) was used to evaluate the performance. The position characteristics obtained through SVM‐RFE (recursive feature elimination) algorithm and LOOCV was considered to compare each feature's relative importance for the prediction model.ResultsCompared with the single‐level prediction model, the ensemble learning prediction model showed improvement in prediction performance (accuracy from 0.61 to 0.86 with imaging data and genetic data). Integrated with 14 priori brain regions, the region of interest (ROI) map ensemble learning prediction model can achieve a performance that is analogous with the model with information from whole‐brain regions (both with accuracy of 0.81). The integration of genetic features further improved the sensitivity of prediction (sensitivity from 0.78 to 0.87 under the ensemble learning framework).Data ConclusionOur ensemble learning prediction model demonstrated significant advantages in interpretability and information integration. The findings may provide more assistance for clinical treatment selection in MDD at the individual level.Level of Evidence: 1Technical Efficacy: Stage 2J. Magn. Reson. Imaging 2020;52:161–171.
Uncovering matrix effects on lipid analyses in MALDI imaging mass spectrometry experiments
Authors: Perry William J. , Patterson Nathan Heath , Prentice Boone M. , Neumann Elizabeth K. , Caprioli Richard M. , Spraggins Jeffrey M. 
Published: December 2019

AbstractThe specific matrix used in matrix‐assisted laser desorption/ionization imaging mass spectrometry (MALDI IMS) can have an effect on the molecules ionized from a tissue sample. The sensitivity for distinct classes of biomolecules can vary when employing different MALDI matrices. Here, we compare the intensities of various lipid subclasses measured by Fourier transform ion cyclotron resonance (FT‐ICR) IMS of murine liver tissue when using 9‐aminoacridine (9AA), 5‐chloro‐2‐mercaptobenzothiazole (CMBT), 1,5‐diaminonaphthalene (DAN), 2,5‐Dihydroxyacetophenone (DHA), and 2,5‐dihydroxybenzoic acid (DHB). Principal component analysis and receiver operating characteristic curve analysis revealed significant matrix effects on the relative signal intensities observed for different lipid subclasses and adducts. Comparison of spectral profiles and quantitative assessment of the number and intensity of species from each lipid subclass showed that each matrix produces unique lipid signals. In positive ion mode, matrix application methods played a role in the MALDI analysis for different cationic species. Comparisons of different methods for the application of DHA showed a significant increase in the intensity of sodiated and potassiated analytes when using an aerosol sprayer. In negative ion mode, lipid profiles generated using DAN were significantly different than all other matrices tested. This difference was found to be driven by modification of phosphatidylcholines during ionization that enables them to be detected in negative ion mode. These modified phosphatidylcholines are isomeric with common phosphatidylethanolamines confounding MALDI IMS analysis when using DAN. These results show an experimental basis of MALDI analyses when analyzing lipids from tissue and allow for more informed selection of MALDI matrices when performing lipid IMS experiments.
Application of electrical resistivity to assess subsurface geological and hydrological conditions at post‐tin mining sites in Indonesia
Authors: Itoh Masayuki , Matsubara Takashi , Shiodera Satomi , Iizuka Kotaro , Sakurai Ken , Nakajima Yoko , Ohashi Hiroki , Manalu Ivan 
Published: December 2019

AbstractIn many parts of Southeast Asia, opencast hydraulic tin mining has been carried out since the colonial era. Regardless of its scale, this mining method has significantly affected microtopography and left extremely degraded soil surfaces, where vegetation can hardly grow. Therefore, the identification of a feasible method for the rehabilitation and reforesting of these areas is a long‐term issue. In this study, we aimed to clarify the subsurface geological (soil properties and distribution) and hydrological conditions at a post‐tin mining site on an Indonesian island using an electrical resistivity survey combined with microtopographic information. The electrical resistivity survey of the study site provided information about the distribution of bedrock and sandy and clayey soils, reflecting mining activities. Furthermore, direct and continuous groundwater level monitoring showed the ranges of groundwater fluctuation depending on the soil and climate conditions. Our study demonstrates that subsurface assessment by electrical resistivity survey can provide essential information, contributing to the development of feasible plans for reforestation or transformation to agricultural land at post‐tin mining sites through the improvement of physical conditions and soil fertility.
An investigation on the effectiveness of hedonic versus utilitarian message appeals in luxury product communication
Authors: Amatulli Cesare , De Angelis Matteo , Donato Carmela 
Published: December 2019

AbstractThis research studies the role of hedonic versus utilitarian message appeals in luxury goods communication, investigating how using one or the other type of message appeal affects product perceived luxuriousness and, in turn, product attitude, and consumers' willingness to buy. This research presents three experiments in which message appeal and brand prominence have been manipulated, while perception of luxuriousness, attitude toward luxury products, willingness to buy them and consumers' conspicuous consumption orientation have been measured. Hedonic, compared with utilitarian, message appeals increase perceived luxuriousness, thereby increasing product attitude and consumers' willingness to buy the product promoted. This effect is particularly likely to occur for consumers with lower levels of conspicuous consumption orientation and for products carrying lowly prominent logos. We extend the literature on luxury communication by studying the effect of hedonic versus utilitarian message appeals on consumers' responses, and the literature on hedonism versus utilitarianism by studying this dichotomy in the context of luxury goods communication. This research suggests that different message appeals used in luxury goods communication produce different effects on consumers' responses and that this differential effectiveness is particularly likely to manifest for certain types of consumers and certain types of luxury products.
Barriers to practicing patient advocacy in healthcare setting
Authors: Nsiah Comfort , Siakwa Mate , Ninnoni Jerry P. K. 
Published: December 2019

AbstractAimTo explore barriers to practicing patient advocacy in healthcare setting.DesignThis study used a qualitative research approach to arrive at the study result.MethodsTwenty‐five Registered Nurses were purposively selected. Semi‐structured interviews were used to collect data and analysed using qualitative content analysis.ResultsThe main theme identified was lack of cooperation between healthcare team, care recipients and the health institution which included the health institution and work environment, ineffective communication and interpersonal relationship, patients' family, religious and cultural beliefs. Unsuccessful advocacy resulted in increased complications, death, negative consequence on the health institution and nursing as a profession. This study has significantly created awareness of the need for an improved patient advocacy to enhance the quality and safety in the care of patients.
Association of Food Access, Recreational Opportunities, and Natural Amenities with Engagement in the Veterans MOVE! Weight Management Program
Authors: Graham Laura A. , Malone Emily B. , Richman Joshua S. , Carson April P. , Affuso Olivia , Knight Sara J. , Levitan Emily B. 
Published: December 2019

ObjectiveThe aim of this study was to examine the association of county‐level food access, recreational opportunities, and natural amenities with participant engagement in a weight management program.MethodsIn this cohort study, participants in the Veterans Health Administration MOVE! weight management program between October 1, 2007, and September 30, 2013, were observed for 12 months after enrollment. Engagement was measured as the number of program visits per year at 12 months. Cross‐sectional analysis and spatial regression were used to examine county characteristics associated with greater participant engagement at 12 months.ResultsA total of 321,624 participants in 2,708 counties were included. Greater engagement was associated with older age, female sex, white race, being married, and being retired. After accounting for similarities between nearby communities, engagement at 12 months was 3.1 visits higher for each additional farmers’ market per 1,000 population (P = 0.01). Engagement was highest for participants living in counties with the most natural amenities (P < 0.001). Recreational opportunities had only a small effect on engagement in the program (β = 0.02 visits at 12 months; P = 0.002).ConclusionsConsideration of a participant’s county characteristics in addition to other known demographics and program factors may help to explain variation in engagement in weight management programs.
Maternal Weight, Weight Gain, and Metabolism are Associated with Changes in Fetal Heart Rate and Variability
Authors: Mat Husin Haliza , Schleger Franziska , Bauer Ilena , Fehlert Ellen , Kiefer‐Schmidt Isabelle , Weiss Magdalene , Kagan Karl Oliver , Brucker Sara , Pauluschke‐Fröhlich Jan , Eswaran Hari , Häring Hans‐Ulrich , Fritsche Andreas , Preissl Hubert 
Published: December 2019

ObjectivePrepregnancy obesity and extensive weight gain can lead to diseases in the offspring later in life. The aim of this study was to evaluate the effect of anthropometric and metabolic factors on the fetal autonomic nervous system (ANS) in uncomplicated pregnancies.MethodsA total of 184 pregnant women in the second or third trimester were included, and for 104 women, maternal insulin sensitivity (ISI) was determined. Fetal heart rate (HR) and heart rate variability (HRV) were determined by magnetic recording. Associations of maternal prepregnancy BMI, weight gain, and ISI with fetal HR and HRV were evaluated by ANCOVA, partial correlation, and mediation analysis.ResultsHR was increased and HRV decreased in fetuses of mothers with overweight or obesity in comparison to normal‐weight mothers. Fetal HR was negatively correlated with maternal weight gain. Maternal prepregnancy BMI was positively correlated with fetal high frequency and was negatively correlated with low frequency and low/high frequency ratio. Maternal ISI showed a negative correlation with fetal HR.ConclusionsThe results show that the fetal ANS is sensitive to alterations of prepregnancy BMI, weight changes, and glucose metabolism. These findings highlight the importance of the intrauterine environment on the developing ANS and the possible programming of obesity.
Visible‐to‐UV Photon Upconversion in Nanostructured Chromophoric Ionic Liquids
Authors: Hisamitsu Shota , Miyano Junji , Okumura Keisuke , Ka‐Ho Hui Joseph , Yanai Nobuhiro , Kimizuka Nobuo 
Published: December 2019

AbstractInvited for this month's cover picture is the group of Professor Nobuhiro Yanai and Nobuo Kimizuka at Kyushu University. The cover picture shows visible‐to‐ultraviolet (vis‐to‐UV) triplet‐triplet annihilation‐based photon upconversion (TTA‐UC) via triplet exciton diffusion in a chromophoric ionic liquid (IL). Chromophore moieties spontaneously form continuous arrays in non‐polar nano‐domains of the IL. The close arrangement of the chromophores in the IL allowed effective diffusion of triplet excitons, resulting in the vis‐to‐UV TTA‐UC at a low excitation intensity. Read the full text of their Communication at 10.1002/open.201900304.
Agrochemical industry development, trends in R&D and the impact of regulation
Authors: Phillips Matthew William Archer 
Published: December 2019

AbstractOver the last 20 years the share of the crop protection market attributable to the leading markets (North America, EU‐15 and Japan) that are the major focus of new active ingredient research and development (R&D) has declined. Greater growth has been recorded in developing markets, questioning the focus of current R&D strategy. R&D budgets within the major companies have seen a shift toward genetically modified (GM) trait development away from agrochemicals, such that the rate of new active ingredients entering development and subsequently being introduced has declined. As a result, the industry has become more reliant on older, off‐patent chemistry, although the availability of older products has been affected by re‐registration requirements, particularly in the EU. Current criteria often preclude the registration of broad‐spectrum agrochemicals, resulting in many new active ingredients being single site active, which is perceived to increase the potential for resistance development, particularly for herbicides, although this is not always the case. © 2019 The Author. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.