bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of Acanthamoeba Spp. Cell‐Free Supernatants on Some Bacterial Pathogens
Authors: Özcan Aykol Şevval Maral , Zeybek Zuhal , Kayabaş Yavuzhan , Çevikli Serranur , Keskin Nihan Berfin , Kahraman Münise Hilal , Çaliş Hümeyra 
Published: December 2024

ABSTRACTThe fact that free‐living amoebae of the genus Acanthamoeba can live in many different environments causes these protozoa to have different interactions with other microorganisms. Investigation of Acanthamoeba–pathogenic bacteria interaction is important for the discovery of new antibacterial agents that can be used against pathogenic bacteria. In this study, it was aimed to investigate the antibacterial effect of cell‐free supernatants obtained from Acanthamoeba against some pathogenic bacteria. One standard strain (Acanthamoeba castellanii ATCC 50373) and one environmental strain (B1) of the genus Acanthamoeba were used in the study. Cell‐free supernatants were obtained by centrifuging the axenic cultures (3000 rpm, 5 min) and passing through a sterile filter with a pore diameter of 0.22 µm. The antibacterial effect of cell‐free supernatants against five different pathogenic bacteria (Acinetobacter baumannii, Klebsiella pneumoniae, Enterococcus faecalis, Salmonella Typhi, and Salmonella enterica) was investigated by colony counting method. As a result of the study, it was determined that the standard Acanthamoeba cell‐free supernatant showed the highest antibacterial effect against E. faecalis (75.79%), while B1 cell‐free supernatant showed the highest antibacterial effect against K. pneumoniae (8.5%). The content of the tested Acanthamoeba cell‐free supernatants was analyzed by gas chromatography/mass spectrometry in our previous study and was also found to contain major compounds with antibacterial properties. Therefore, it is thought that the metabolites produced by Acanthamoeba can be used as an alternative to existing antimicrobial drugs in the fight against infections caused by some important pathogenic bacteria.
A rare case of pancreatic mucinous cystic neoplasm in a pediatric patient
Authors: Colak Muhammed Ali , Freeman A. Jay , Heinzman Christie , Khan Muhammad A. , Mangray Shamlal , Potter Carol J. , Rasmussen Sara K. , Rees Mitchell A. , Nathan Jaimie D. 
Published: December 2024

AbstractPancreatic mucinous cystic neoplasms (MCNs) are rare tumors predominantly identified in middle‐aged females. Occurrences are extremely rare in children. We report a rare case of pancreatic MCN in an adolescent. A 14‐year‐old female with Smith–Magenis syndrome and steatotic liver disease (SLD) presented with abdominal discomfort. Magnetic resonance elastography obtained for SLD revealed cholelithiasis and a 0.5‐cm cyst in the distal pancreas. Observation was recommended by her pediatric surgery provider. At 18 years old, contrast‐enhanced abdominal magnetic resonance imaging showed a 2.4‐cm mildly complex, septate, cystic lesion along the distal pancreas with no soft tissue component, pancreatic atrophy, or ductal dilation. Endoscopic ultrasound with cyst fluid cytology showed no definitive neoplasm. Carcinoembryonic antigen level of cyst fluid was markedly elevated at 11,207 ng/mL, concerning an MCN. She was referred to Hepatopancreatobiliary Surgery for evaluation and underwent robotic‐assisted distal pancreatectomy. Final pathology revealed a 2.8‐cm low‐grade MCN with intermediate‐grade dysplasia.
Developing a Scale to Measure Need‐to‐Tell Personality Trait to Predict Consumers' Willingness to Provide Online Reviews
Authors: Kim John , Thrasher Gregory R. , Demsky Caitlin A. 
Published: December 2024

ABSTRACTThere is little debate that consumers' online reviews have a significant impact on corporate sales and profit. Thus, companies must create an overall strategy to manage online posting, and one important aspect of the strategy is to formulate an online review acquisition plan. An online review acquisition plan requires an in‐depth understanding of posting behavior. While past research has focused on motives to explain why consumers post online reviews, to create a more comprehensive understanding of online posting behavior recent research has called for a deeper examination of the relationship between personality and posting behavior. To address the need for more context‐specific personality predictors of online reviews, we propose a personality construct called “need to tell” and develop a scale to measure this construct. We conducted three studies to develop items and establish scale psychometric properties (Study 1), establish construct validity in reference to the nomological net of need to tell (Study 2), and establish criterion and incremental criterion validity with relevant online posting outcomes (Study 3). Our results indicate that the developed measure of need to tell is both reliable and associated with popular online posting behavior metrics. This newly developed scale can be used when segmenting consumers to identify those most likely to be impacted by nudges to post online reviews.
Metal‐ and Halide‐Free, Macroporous Hygroscopic Polymers for Efficient Atmospheric Water Harvesting
Authors: Ju Aiming , Xu Zhiguang , Huang Zhihao , Zhang Tao , Zhao Yan 
Published: December 2024

AbstractHygroscopic materials based on highly hygroscopic salts are promising for atmospheric water harvesting (AWH), but the metal‐ or halide‐containing highly hygroscopic salts often have leakage and corrosion issues. Here, the design and synthesis of metal‐ and halide‐free, highly hygroscopic, and macroporous polymers from [2‐(acryloyloxy)ethyl]trimethylammonium chloride simply via in situ foaming, solidification, and ion exchange are reported. The resulting polymers exhibit highly interconnected macroporous structure, robust compression, and leakage‐free performance, and they also demonstrate relatively high moisture adsorption capacities (up to 1.24 g g−1 water at 85% relative humidity, accelerated adsorption rates, and efficient desorption. The polymers can harvest 0.76 g g−1 water per adsorption–desorption cycle and show high reusability, without obvious deterioration over 10 cycles of adsorption, desorption, and water collection. With the incorporation of carboxylic multi‐walled carbon nanotubes into the hygroscopic polymers, solar‐driven AWH is realized, without the requirement of additional energy. On the whole, the metal‐ and halide‐free, highly hygroscopic polymers exhibit the advantages of ease of preparation, energy saving, environmental protection, and economic sustainability for AWH.
A retrospective observational study of patients with melanoma prescribed combined BRAF and MEK inhibitors in a real‐world treatment setting in Japan
Authors: Namikawa Kenjiro , Ngew Kok Yew , Lukowicz Zuzanna , Kano Ryosuke 
Published: December 2024

AbstractObjectiveThis study aimed to describe patients with melanoma initiating treatments with dabrafenib plus trametinib (Dab + Tram) or encorafenib plus binimetinib (Enco + Bini) in a real‐world setting in Japan.MethodsData were extracted from the Japanese Medical Data Vision (MDV) insurance claims database. Patients diagnosed with melanoma between 2012 and 2021 and prescribed with Dab + Tram or Enco + Bini were included in three cohorts: non‐adjuvant Dab + Tram, adjuvant Dab + Tram, and Enco + Bini. Data were extracted on patient characteristics at treatment initiation. During follow‐up, all changes in melanoma treatments were documented. Treatment adherence was determined as the proportion of prescription days covered (PDC) and treatment dose intensity as the relative dose intensity (RDI).ResultsSixty‐seven patients were included in the non‐adjuvant Dab + Tram cohort (55 first‐line treatments), seven in the adjuvant Dab + Tram cohort (six first‐line treatments), and 16 in the Enco + Bini cohort (four first‐line treatments). The mean age was 61.3 ± 13.5 years and 56.1% were men. Twenty‐seven patients with non‐adjuvant Dab + Tram or Enco + Bini in first line (45.8%) switched to a second line. The median treatment duration was 11.8 months for Dab + Tram and 8.1 months for Enco + Bini. A PDC ≥ 80% was observed for 85.7% of patients with adjuvant Dab + Tram, 68.7% for non‐adjuvant Dab + Tram, and 75.0% for Enco + Bini. Median RDI was 1.0 for adjuvant Dab + Tram, 0.9 for non‐adjuvant Dab + Tram, and 0.6 for Enco + Bini.ConclusionDab + Tram is used consistently with clinical practice guidelines in the adjuvant setting, but adherence in the non‐adjuvant setting is suboptimal, as is the prescribed dose of Enco + Bini. Prescribers should ensure that these therapies are used in an optimal way to improve outcomes in melanoma.
An Artifact in Concentric Needle EMG Recordings
Authors: Nandedkar Sanjeev D. , Barkhaus Paul E. 
Published: December 2024

ABSTRACTIntroduction/AimsIn healthy subjects, we observed high amplitude motor unit potential (MUP) waveforms that resembled the cannula potential (CP) with a positive sharp wave (PSW)‐like waveform. We analyzed the source of this signal, its prevalence, and its effects on the analysis of electromyographic waveforms.MethodsThree channel recordings were performed to explore the contribution of the needle core and cannula to the MUP. In 7 control subjects with no neuromuscular disease, the EMG interference pattern was quantified for the amplitude asymmetry (AASM) of its positive and negative going peaks.ResultsThe large amplitude MUP resembling a CP with a PSW‐type waveform is recorded by the needle core, not the cannula. With a slight change in needle position, its amplitude decreased and the waveform had a normal appearance. Thus, it is an artifact. Such potentials (AASM > 150%) are commonly encountered (frequency 7%) and occur in all muscles and subjects in this study.DiscussionThe artifact is an enigma and appears related to the interaction between the needle and muscle fiber(s) similar to that in PSW signals. Failure to recognize these waveforms may result in a false impression of abnormality. AASM calculations can help confirm this artifact in automated analysis algorithms to preclude false positive neurogenic patterns.
Effects of exploitation and emigration on apparent survival of Walleye in Lake Sharpe, South Dakota
Authors: Sacco Laurel H. , Fincel Mark J. , Goble Cameron W. , Davis Tanner , Chipps Steven R. 
Published: December 2024

AbstractObjectiveIn 2017, we began a 5‐year mark–recapture study to estimate apparent survival of Walleye Sander vitreus and angler exploitation in Lake Sharpe, South Dakota, and inform management strategies for this fishery. The study revealed substantial downstream emigration of Walleye; therefore, we also examined the influence of emigration on apparent mortality.MethodsWe estimated Walleye apparent survival using the Seber parameterization of the dead recovery model. In our models, we included factors influencing voluntary fish release, as well as harvest restrictions, tagging location, and year. We used a multistate Markov model to estimate emigration in relation to Walleye total length, sex, and tagging location, as well as annual reservoir discharge. The exploitation and emigration estimates informed our interpretations of annual Walleye apparent survival.ResultApparent survival patterns were explained by an interaction between Walleye length and a minimum harvest length restriction. Apparent survival was lower during July and August, when the minimum harvest length limit was suspended, and results suggest that anglers were willing to release preferred sizes (≥508 mm) when able to harvest smaller (<381 mm) Walleye. Annual apparent survival, exploitation, and emigration estimates ranged between 15% and 47%, 8% and 25%, and 11% and 28%, respectively. Emigration was influenced by tagging location and annual discharge: increasing with proximity to Big Bend dam and during high annual flows. Although years with high exploitation or emigration corresponded to years with low apparent survival, estimates did not explain the magnitude of mortality or emigration indicated by survival models.ConclusionOur results revealed that harvest–release decisions by Walleye anglers in Lake Sharpe are primarily influenced by regulations, although exploitation was not a significant source of mortality. Annual patterns in apparent survival indicate high mortality or emigration. Continued investigations into immigration and emigration and sources of mortality of Lake Sharpe Walleye are warranted to better inform management strategies for the fishery.
Quaternion Tensor Completion via QR Decomposition and Nuclear Norm Minimization
Authors: Sun Jian , Liu Xin , Zhang Yang 
Published: December 2024

ABSTRACTThe task of tensor (matrix) completion has been widely used in the fields of computer vision and image processing, etc. To achieve the completion, the existing methods are mostly based on singular value decomposition of the real tensors and nuclear norm minimization. However, the real tensor completion methods cannot simultaneously maintain color channel correlation and evolution robustness of color video frames, and they need high computational costs to handle the high‐dimensional data. Hence they have some limitations in model generalization ability and computational efficiency. In this article, a new completion method for the quaternion tensor (matrix) is explored via the QR decomposition and the definition of novel quaternion tensor norm, which can well balance the model generalization ability and efficiency, and the performance of the completion method has been substantially improved. Numerical experiments on color images and videos prove the effectiveness of our proposed method.
Effects of short‐term exposure to moderate amounts of alcohol on brain volume
Authors: Tsugawa Sakiko , Ueno Fumihiko , Sakuma Mutsuki , Tani Hideaki , Ochi Ryo , Graff‐Guerrero Ariel , Noda Yoshihiro , Uchida Hiroyuki , Mimura Masaru , Oshima Shunji , Matsushita Sachio , Nakajima Shinichiro 
Published: December 2024

AbstractAimAlthough numerous studies have reported that chronic alcohol consumption causes brain volume reduction and cerebrospinal fluid volume increase, few studies have examined the acute effects of alcohol on brain structure. This study aims to investigate the short‐term brain volume changes following alcohol administration.MethodsModerate doses of alcohol were administered intravenously to 18 healthy volunteers for a total of 90 min to achieve a blood alcohol concentration of 0.5 mg/mL. An alcohol clamp method combined with physiologically based pharmacokinetic modeling was used to achieve fine control over blood alcohol concentration. T1 images with 3T MRI were scanned at three time points: baseline, 0 min, and 90 min after the end of alcohol administration. Cortical, subcortical, and ventricular volumes were computed after segmentation with FreeSurfer. Repeated measures analysis of variance was used to evaluate longitudinal changes in brain volume at 96 regions.ResultsAcute alcohol administration increased bilateral lateral ventricular volumes, which lasted until 90 min after the end of alcohol injection. On the other hand, the volumes of total gray matter, left precentral cortex, left caudal middle frontal cortex, and left superior frontal cortex decreased after alcohol administration, but these changes disappeared 90 min after the end of alcohol administration.ConclusionAcute injection of moderate doses of alcohol may enlarge ventricle volumes and reduce gray matter volumes. The transient volume changes caused by acute administration of alcohol may be related to changes in CSF flow and water content of brain tissue, which warrants further study.
Adaptive Fuzzy Decentralized Output‐Feedback Inverse Optimal Control for Nonstrict‐Feedback Interconnected Large‐Scale Systems
Authors: Yang Shumeng , Wu Wei , Tong Shaocheng 
Published: December 2024

ABSTRACTIn this article, the problem of adaptive fuzzy decentralized inverse optimal control is investigated for nonstrict‐feedback nonlinear interconnected large‐scale systems. The interconnected large‐scale systems include unmeasurable states, nonstrict‐feedback structures, and unknown nonlinear functions. Fuzzy logic systems are used to approximate unknown nonlinear functions of subsystems, and a fuzzy decentralized state observer is proposed to estimate unmeasured states. By combining the dynamic surface backstepping control technique with inverse optimal control principle, an adaptive fuzzy decentralized inverse optimal control scheme is established. It is proved that all the signals of the controlled nonlinear interconnected large‐scale systems are bounded, the tracking errors converge to a small neighborhood of zero, and the cost function is minimized. The simulation results and comparison results further verify the effectiveness of the proposed decentralized inverse optimal control approach.
Fabricating a novel bioinspiring 3D‐printed sodium alginate/silk fibroin/polyvinyl alcohol/nano‐hydroxyapatite composite scaffold structure for bone tissue engineering application
Authors: Majhi Partha Sarathi , Pramanik Krishna , Singh Amit Kumar 
Published: December 2024

AbstractIn the present work, we fabricated 3D‐printed biofunctionalized nanocomposite scaffold structures using bioinks prepared by incorporating different concentrations (0.5%–1.5% w/v) of nano‐hydroxyapatite (n‐HA) into optimized a tri‐polymer complex blend comprising of sodium alginate (SA), silk fibroin (SF), polyvinyl alcohol (PVA) with composition represented as SA70/SF30/PVA3, wherein, SA/SF is in 70:30 (v/v) ratio and PVA is in 3% (w/v) ratio. The printed scaffolds consist of microfibers with fiber diameter and pore size range of 437–806 and 389–446 μm having pore interconnecting networks as assessed by scanning electron microscope image analysis. However, with increased n‐HA content, the formation of a microfibrous porous structure with interconnected pores. The amorphous and hydrophilic characteristics of the scaffolds were revealed by XRD analysis and contact angle values ranging from 52 ± 2° to 66 ± 1°. SA70/SF30/PVA3 scaffold containing 1.5% (w/v) n‐HA has shown a controlled degradation pattern. The highest compressive strength of 0.193 MPa was shown by SA70/SF30/PVA3/n‐HA1.5 scaffold. The fabricated scaffolds are cytocompatible, as evident from cell adhesion and MTT assays. Among the scaffolds, the SA70/SF30/PVA3/n‐HA1.5 scaffold exhibited better biological activities as evaluated by an in vitro cell study. A higher alkaline phosphatase activity signifying superior osteogenic performance of the SA70/SF30/PVA3/n‐HA1.5 scaffold could be a favorable substitute for bone tissue engineering applications.Highlights
Nano hydroxyapatite provided structural stability to the scaffold.
Controlled degradation pattern of the fabricated scaffold.
Higher compressive strength of the scaffold.
Hydrophilic and amorphous nature of the scaffold.
The scaffold has a favorable environment for bone tissue regeneration.
Degradable Pressure‐Sensitive Adhesive Prepared From CO2‐Based Polycarbonate
Authors: Zheng Xin‐Peng , Wang Wen‐Zhen , Cui Yu‐Kun , Jia Xin‐Gang , Li Hong‐Jiu , Liu Xin‐Yi , Chen Huan‐Ping , Mao Zheng‐Hao 
Published: December 2024

ABSTRACTPressure‐sensitive adhesives (PSAs) are widely used in daily life, but their poor degradability poses significant challenges to sustainable development. To address this, we synthesized a polycarbonate, PPCB, using carbon dioxide and propylene oxide as raw materials, incorporating rigid benzene rings and ester functional groups. PPCB exhibited a T
d,−5%, and T
d,max that were 58.5% and 30.7% higher than those of polypropylene carbonate (PPC), respectively. Its highest tensile strength was 55% higher than PPC, while its elongation at break decreased from 421% to 165%. The 180° peel strength of PPCB reached 5.2 ± 0.56 N/cm, surpassing that of some commercial products such as Duct‐tape. PPCB demonstrated high and stable adhesion strength (~52 N) on various substrates. Its adhesion strength to skin tissue was 26.6 ± 1.6 kPa, significantly higher than that of commercially available fibrin glue. Rheological studies indicated that PPCB maintained high viscosity throughout the testing process, with a lap shear strength close to 4.56 MPa. Additionally, PPCB showed excellent degradability, with a high water absorption rate and significant weight loss. PPCB also exhibited good biocompatibility, with a cell viability rate greater than 90%. PPCB has the potential to become a novel degradable PSA, providing a new solution for environmental sustainability.
The Risk of Pneumothorax With Intrapleural Urokinase in Children With Parapneumonic Effusion
Authors: Kadmon Gili , Schoen Adi , Nahum Elhanan , Weissbach Avichai , Kaplan Eytan , Cohen Tal , Chodick Gabriel , Scheuerman Oded 
Published: December 2024

ABSTRACTAimFibrinolytic therapy is commonly used in children with parapneumonic effusion, to facilitate drainage of the effusions and recovery. However, data regarding complications of this treatment in children are limited. We aimed to determine the incidence of pneumothorax (PNX) associated with intrapleural urokinase.MethodsWe analyzed retrospectively collected data of children with parapneumonic effusion who underwent chest drain insertion. The clinical course and complications, including the incidence of PNX, were compared between children who were and were not treated with urokinase.ResultsThe study group included 120 children, of whom 57 were treated with urokinase. Children who were and were not treated with urokinase did not differ in markers of disease severity or in the length of hospitalization. Among the patients treated with urokinase compared to those not treated, the incidence of PNX was higher (35% vs. 6%, p < 0.001) and the median duration of chest drain treatment was longer (6 vs. 4 days, p < 0.001).ConclusionIn our pediatric cohort, intrapleural urokinase was associated with a higher incidence of PNX and did not shorten the duration of hospitalization.
Colicin Immunity Proteins of Pathogenic Bacteria Detected by Antibiotic‐Induced SOS Response, Plasmid Sequencing, MALDI‐TOF‐TOF Mass Spectrometry, and Top‐Down Proteomic Analysis
Authors: Fagerquist Clifton K.  , Shi Yanlin  , Park Jihyun  
Published: December 2024

ABSTRACTRationale:Plasmids can play a major role in the survival of pathogenic bacteria. Plasmids are acquired through horizontal gene transfer resulting in their spread across various strains, species and genera of bacteria. Colicins are bacterial protein toxins expressed by plasmid genes and released against co‐located bacterial competitors.Methods:Three Shiga toxin‐producing E. coli (STEC), whose genomes were sequenced previously, were analyzed using a combination of antibiotic induction, MALDI‐TOF‐TOF mass spectrometry, top‐down proteomic analysis, and small plasmid sequencing. Protein biomarkers were identified using in‐house software that matches protein mass and fragment ions of backbone cleavage by the aspartic acid effect. Predicted in silico protein structures assisted in the interpretation of protein ion fragmentation.Results:In addition to proteomic identification of phage‐encoded Shiga toxin, we were able to identify plasmid‐encoded immunity proteins for colicin D and E3. The genes for these plasmid‐encoded proteins were not found in the previous genomic sequencing. However, resequencing of these strains for small plasmids revealed the genes to be present on 7–8 kb sized plasmids. Upstream of the colicin/immunity genes was an inverted repeat of the SOS/LexA box that represses gene expression until antibiotic challenge.Conclusions:Our top‐down proteomic method demonstrates that it is possible to screen putative pathogenic bacteria (whose genomes have been sequenced in full, in part or not at all) for the presence of phage‐ and plasmid‐encoded toxin and colicin genes under SOS control. Small plasmid sequencing confirmed the presence of colicin/immunity genes (and their regulatory control) suggested from induction and top‐down proteomic analysis.