bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of maternal organic selenium supplementation during pregnancy on sow reproductive performance and long-term effect on their progeny
Authors: Mou Daolin , Ding Dajiang , Li Shuang , Yan Hui , Qin Binting , Li Zhen , Zhao Lianpeng , Che Lianqiang , Fang Zhengfeng , Xu Shengyu , Lin Yan , Zhuo Yong , Li Jian , Huang Chao , Zou Yuanfeng , Li Lixia , Briens Mickael , Wu De , Feng Bin 
Published: November 2020

Abstract
Selenium (Se) is an essential trace element for animals and exists in nature in both inorganic and organic forms. Although organic Se is more bioavailable than inorganic Se, there are inconsistent reports on the effect of organic Se on the reproductive performance of sows. This study was conducted to investigate the effect of maternal organic Se (2-hydroxy-4-methylselenobutanoic [HMSeBA]) supplementation on reproductive performance and antioxidant capacity of sows, and the long-term effect on the growth performance and antioxidant capacity of their offspring with or without lipopolysaccharide (LPS) challenge. The experimental design used in this study was a completely randomized design; 45 Landrace × Yorkshire sows were randomly allocated to receive one of the following three diets during gestation: control diet (Control, basal diet, n = 15), sodium selenite (Na2SeO3)-supplemented diet (Na2SeO3, basal diet + 0.3 mg Se/kg Na2SeO3, n = 15), and HMSeBA-supplemented diet (HMSeBA, basal diet + 0.3 mg Se/kg HMSeBA, n = 15). On day 21 of age, male offspring from each group were injected with LPS or saline (n = 6). As compared with the control group, maternal HMSeBA supplementation increased the number of total born piglets, while decreased birth weight (P &lt; 0.05). In the first week of lactation, maternal HMSeBA supplementation increased litter weight gain compared with the Na2SeO3 group (P &lt; 0.05) and increased the average daily gain of piglets compared with the control group and Na2SeO3 group (P &lt; 0.05). Meanwhile, maternal HMSeBA supplementation decreased piglet birth interval as compared with the control group and Na2SeO3 group (P &lt; 0.05). Besides, plasma glutathione peroxidase (GSH-Px) activity was higher in the HMSeBA group on farrowing 0 min and 90 min, while malondialdehyde (MDA) concentration was lower on farrowing 0, 90, and 135 min than those in the control group (P &lt; 0.05). In addition, maternal HMSeBA supplementation increased the concentration of selenoprotein P (SELENOP) in colostrum compared with the control group (P &lt; 0.05). Further study revealed that the LPS-challenged HMSeBA group had higher GSH-Px and total antioxidant capacity and lower MDA in weaning piglets compared with the LPS-challenged control group (P &lt; 0.05). Taken together, maternal HMSeBA supplementation increased the number of total born piglets, shortened the duration of farrowing, improved the antioxidant capacities of sows and their offspring, and improved the growth performance of suckling pigs at the first week of lactation. Thus, HMSeBA supplementation during gestation has the potentiality to produce more kilogram of meat.
Abnormally High Blood Acetaldehyde Concentrations Suggest Potential Postmortem Ethanol Generation
Authors: Chen Xiaomin , Dong Xiaoru , Zhu Rongzhe , Xue Qiupeng , Zhang Dingang , Liu Xiaochen , Zheng Lixing , Jiang Yan 
Published: November 2020

Abstract
Ethanol is one of the most commonly used and abused substances worldwide. Identifying whether the source of ethanol detected in corpses is antemortem ingestion or postmortem generation is especially important for determining the cause of death, which remains a vibrant field of research. During the synthesis of ethanol in the putrefaction process of corpses, other small molecules such as acetaldehyde and n-propanol could also be produced. According to our prospective statistical analysis based on authentic samples from forensic cases, it is rational to suspect ethanol generation after death when the concentration of acetaldehyde detected in blood exceeds 0.014 g/dL. Through in vitro simulation experiments, in addition to confirming that ethyl glucuronide and ethyl sulfate are the reliable biomarkers of antemortem ingestion of ethanol, we propose that acetaldehyde is far more sensitive than n-propanol as a potential marker in the blood of corpses for postmortem ethanol formation.
Assessment of Capsule Endoscopy Utilizing Capsocam Plus in Patients With Suspected Small Bowel Disease Including Pilot Study With Remote Access Patients During Pandemic
Authors: Enns Chasyn , Galorport Cherry , Ou George , Enns Robert 
Published: November 2020

Abstract

Background
CapsoCam Plus is a capsule endoscopy (CE) system that utilizes four cameras to capture a panoramic view. This has theoretical advantage over conventional forward-viewing CE with limited field of view. Its ease of administration without requiring any additional equipment during the recording also provides a unique opportunity for patients to self-administer the test. We aimed to evaluate real-life experience using this novel system and to determine feasibility of a remote access program.

Methods
Retrospective chart review was conducted for consecutive adult outpatients who underwent CE using CapsoCam Plus. Patients with significant challenges for in-person procedures were selected for remote access through mail courier services. Gastric transit time, small bowel transit time, completion rate, diagnostic yield and adverse events were compared between remote access versus usual practice.

Results
Ninety-four patients (52.1% male) were included, with 28 in remote access program. Most common indication was gastrointestinal bleeding (85.1%). Complete examination was achieved in 87 patients. Five (5.3%) patients’ capsule remained in stomach during the recording, while two (2.1%) patients missed capsule retrieval. Median small bowel and gastric transit times were 231.9 (interquartile range [IQR] 169.5–308.2) and 27.6 (IQR 13.8–63.5) minutes, respectively. Diagnostic yield was 23.4%. There was no difference in completion rate or transit times between two groups, but diagnostic yield was higher in remote access group (odds ratio 3.80, 95% confidence interval 1.28–11.31). One patient required elective endoscopic retrieval of capsule.

Conclusion
CapsoCam Plus can be safely administered remotely with a high degree of success, which may facilitate timely investigations while limiting nonessential physical interactions during pandemic.
Why does the plateau zokor (Myospalax fontanieri: Rodentia: Spalacidae) move on the ground in summer in the eastern Qilian Mountains?
Authors: Chu Bin , Ji Chengpeng , Zhou Jianwei , Zhou Yanshan , Hua Limin 
Published: November 2020

AbstractSubterranean rodents spend most of their lives in underground burrow systems, and most studies to date on their morphological, behavioral, and physiological, adaptations have been based on their whole-life existence in subterranean ecotopes. However, a few studies have reported that some subterranean rodents exhibit aboveground activity under particular circumstances, and the results of these studies help zoologists to better understand the adaptations of subterranean rodents coping both with belowground and aboveground environments. These studies, however, do not include direct observations of the aboveground activity of these rodents. Moreover, studies into the factors that influence the movement of these subterranean rodents outside of their burrow systems are relatively scarce. Here, we report the aboveground activity pattern of plateau zokor (Myospalax fontanieri) based on videos and photographs captured by infrared cameras combined with radiotracking technology in the eastern Qilian Mountains, northwest China. In addition, the potential factors that influence the aboveground activity of plateau zokor, including temperature, humidity, food, dispersal, and mating, were studied in 2015 and 2016. We found that, of the 16 zokors with radiocollars, five females moved aboveground during the day and night in June and July 2015, with the frequency of their aboveground activity being higher during the day than that at night. Temperature, humidity, mating, and dispersal, had no effects on aboveground plateau zokor movement. However, results of a binary logistic regression indicated that the crude fat contents of aboveground and belowground plants were positively and negatively correlated with the aboveground activity of these zokors, respectively. We infer that plateau zokor move on the ground to forage for plants with higher fat content and that changes in available nutrients might serve as potential cues that affect the surface activity of plateau zokor.
Activity patterns of tayra (Eira barbara) across their distribution
Authors: Villafañe-Trujillo Álvaro José , Kolowski Joseph M , Cove Michael V , Medici Emilia Patricia , Harmsen Bart J , Foster Rebbeca J , Hidalgo-Mihart Mircea G , Espinosa Santiago , Ríos-Alvear Gorky , Reyes-Puig Carolina , Reyes-Puig Juan Pablo , da Silva Xavier Marina , Paviolo Agustín , Cruz Paula , López-González Carlos Alberto 
Published: November 2020

AbstractSpecies’ activity patterns are driven by the need to meet basic requirements of food, social interactions, movement, and rest, but often are influenced by a variety of biotic and abiotic factors. We used camera-trap data to describe and compare the activity patterns of the relatively poorly studied tayra (Eira barbara) across 10 populations distributed from the south of Mexico to the north of Argentina, and attempted to identify biotic or abiotic factors that may be associated with variation in level of diurnality. In a subset of sites we also aimed to document potential seasonal variation in activity. We used a kernel density estimator based on the time of independent photographic events to calculate the proportion of diurnal, crepuscular, and nocturnal activity of each population. Tayras were mostly active during diurnal periods (79.31%, 759 records), with a lower proportion of crepuscular activity (18.07%, 173 records) yet we documented some variation in patterns across the 10 study areas (activity overlap coefficient varied from Δ 4 = 0.64 to Δ 1 = 0.95). In northern localities, activity peaked twice during the day (bimodal) with most activity ocurring in the morning, whereas closer to the geographical equator, activity was constant (unimodal) throughout the day, peaking at midday: activity either was unimodal or bimodal in southern localities. Despite investigating multiple potential abiotic and biotic predictors, only latitude was associated with variation in the proportion of diurnal activity by tayras across its range, with increased diurnal activity closer to the equator. Seasonal comparisons in activity showed a tendency to reduce diurnality in dry versus rainy seasons, but the pattern was not consistently significant. This is the most comprehensive description of tayra activity patterns to date, and lends novel insight into the potential flexibility of the species to adapt to local conditions.
Fine Structure of Maxillary Palps in Adults of Hermetia illucens (Diptera: Stratiomyidae)
Authors: Pezzi M , Scapoli C , Bharti M , Faucheux M J , Chicca M , Leis M , Marchetti M G , Mamolini E , Salvia R , Falabella P , Bonacci T 
Published: November 2020

Abstract
A relevant species in waste management but also in forensic, medical, and veterinary sciences is the black soldier fly, Hermetia illucens (Linnaeus; Diptera: Stratiomyidae). An ultrastructural study by scanning electron microscopy (SEM) was conducted for the first time on maxillary palps of both sexes, describing in detail the morphology and distribution of sensilla and microtrichia. The maxillary palps, composed of two segments, show sexual dimorphism in length and shape. In both sexes, the first segment is covered only by microtrichia, but the second one is divided into two parts: the proximal one, covered only by microtrichia, and the distal one containing both microtrichia and sensory structures. These structures include two types of sensory pits and one of chaetic sensilla. Due to sexual dimorphism in palp size, females have a higher number of sensory pits. The sexual dimorphism of palps and the presence and role of sensilla in H. illucens was discussed in comparison to other species of the family Stratiomyidae and of other Diptera. This study may represent a base for further investigations on mouthpart structures of this species, involved in key physiological activities, such as feeding, mating and oviposition.
Diagnostic evaluation of serum (1, 3)-β-d-glucan levels using the Fungitec G-Test MK kit for Pneumocystis jirovecii pneumonia (PCP) in non-HIV patients
Authors: Ideguchi Shuhei , Yamamoto Kazuko , Hirayama Tatsuro , Takazono Takahiro , Imamura Yoshifumi , Miyazaki Taiga , Sakamoto Noriho , Izumikawa Koichi , Yanagihara Katsunori , Morimoto Shimpei , Mukae Hiroshi 
Published: November 2020

Abstract
Pneumocystis jirovecii pneumonia (PCP) is an opportunistic and life-threatening pulmonary infection with an increasing prevalence among individuals who are human immunodeficiency virus (HIV)-negative. Evidence regarding diagnostic testing of PCP in this patient population is insufficient. We evaluated the performance of serum (1, 3)-β-d-glucan (BDG) using the Fungitec G-test MK kit for diagnosing PCP in non-HIV patients. We retrospectively analyzed data from 219 non-HIV adult patients who underwent bronchoscopy and were tested for P. jirovecii DNA by PCR using lavage samples from the lower respiratory tract. Fifty PCP patients and 125 non-PCP patients were included. The most common underlying diseases were malignancies and systemic autoimmune diseases. Using the serum BDG Fungitec G-test MK test to diagnose PCP, the area under the receiver operating characteristic curve (AUC) was 0.924, whereas the modified cut-off value of 36.6 pg/mL had a sensitivity and specificity of 92.0% and 84.8%, respectively. The AUC for patients with systemic autoimmune diseases was 0.873, and the accuracy of serum BDG test declined when using methotrexate (MTX). In conclusion, the serum BDG test was useful for diagnosing PCP in non-HIV patients; however, the results should be carefully interpreted in case of MTX administration.
Fast photometric variability of very low mass stars in IC 348: detection of superflare in an M dwarf
Authors: Ghosh Samrat , Mondal Soumen , Dutta Somnath , Das Ramkrishna , Joshi Santosh , Lata Sneh , Khata Dhrimadri , Panja Alik 
Published: November 2020

ABSTRACT
We present here optical I-band photometric variability study down to ≃19 mag of a young (∼2–3 Myr) star-forming region IC 348 in the Perseus molecular cloud. We aim to explore the fast rotation (in the time-scales of hours) in very low-mass stars including brown dwarfs (BDs). From a sample of 177 light curves using our new I-band observations, we detect new photometric variability in 22 young M dwarfs including 6 BDs, which are bonafide members in IC 348 and well characterized in the spectral type of M dwarfs. Out of 22 variables, 11 M dwarfs including one BD show hour-scale periodic variability in the period range 3.5–11 h and rest are aperiodic in nature. Interestingly, an optical flare is detected in a young M2.75 dwarf in one night data on 2016 December 20. From the flare light curve, we estimate the emitted flared energy of 1.48 × 1035 erg. The observed flared energy with an uncertainty of tens of per cent is close to the superflare range (∼1034 erg), which is rarely observed in active M dwarfs.
Probing the nature of dark matter with accreted globular cluster streams
Authors: Malhan Khyati , Valluri Monica , Freese Katherine 
Published: November 2020

ABSTRACT
The steepness of the central density profiles of dark matter (DM) in low-mass galaxy haloes (e.g. dwarf galaxies) is a powerful probe of the nature of DM. We propose a novel scheme to probe the inner profiles of galaxy subhaloes using stellar streams. We show that the present-day morphological and dynamical properties of accreted globular cluster (GC) streams – those produced from tidal stripping of GCs that initially evolved within satellite galaxies and later merged with the Milky Way (MW) – are sensitive to the central DM density profile and mass of their parent satellites. GCs that accrete within cuspy cold dark matter (CDM) subhaloes produce streams that are physically wider and dynamically hotter than streams that accrete inside cored subhaloes. A first comparison of MW streams ‘GD-1’ and ‘Jhelum’ (likely of accreted GC origin) with our simulations indicates a preference for cored subhaloes. If these results hold up in future data, the implication is that either the DM cusps were erased by baryonic feedback, or their subhaloes naturally possessed cored density profiles implying particle physics models beyond CDM. Moreover, accreted GC streams are highly structured and exhibit complex morphological features (e.g. parallel structures and ‘spurs’). This implies that the accretion scenario can naturally explain the recently observed peculiarities in some of the MW streams. We also propose a novel mechanism for forming ‘gaps’ in stellar streams when the remnant of the parent subhalo (which hosted the GC) later passes through the GC stream. This encounter can last a longer time (and have more of an impact) than the random encounters with DM subhaloes previously considered, because the GC stream and its parent subhalo are on similar orbits with small relative velocities. Current and future surveys of the MW halo will uncover numerous faint stellar streams and provide the data needed to substantiate our preliminary tests with this new probe of DM.
Cosmological perturbations for two cold fluids in ΛCDM
Authors: Rampf Cornelius , Uhlemann Cora , Hahn Oliver 
Published: November 2020

ABSTRACT
The cosmic large-scale structure of our Universe is comprised of baryons and cold dark matter (CDM). Yet it is customary to treat these two components as a combined single-matter fluid with vanishing pressure, which is justified only for sufficiently large scales and late times. Here, we go beyond the single-fluid approximation and develop the perturbation theory for two gravitationally coupled fluids while still assuming vanishing pressure. We mostly focus on perturbative expansions in powers of D (or D+), the linear structure growth of matter in a ΛCDM Universe with cosmological constant Λ. We derive in particular (1) explicit recursion relations for the two fluid densities, (2) complementary all-order results in the Lagrangian-coordinates approach, as well as (3) the associated component wavefunctions in a semiclassical approach to cosmic large-scale structure. In our companion paper, we apply these new theoretical results to generate novel higher order initial conditions for cosmological hydrodynamical simulations.
Eusociality Shapes Convergent Patterns of Molecular Evolution across Mitochondrial Genomes of Snapping Shrimps
Authors: Chak Solomon T C , Baeza Juan Antonio , Barden Phillip 
Published: November 2020

AbstractEusociality is a highly conspicuous and ecologically impactful behavioral syndrome that has evolved independently across multiple animal lineages. So far, comparative genomic analyses of advanced sociality have been mostly limited to insects. Here, we study the only clade of animals known to exhibit eusociality in the marine realm—lineages of socially diverse snapping shrimps in the genus Synalpheus. To investigate the molecular impact of sociality, we assembled the mitochondrial genomes of eight Synalpheus species that represent three independent origins of eusociality and analyzed patterns of molecular evolution in protein-coding genes. Synonymous substitution rates are lower and potential signals of relaxed purifying selection are higher in eusocial relative to noneusocial taxa. Our results suggest that mitochondrial genome evolution was shaped by eusociality-linked traits—extended generation times and reduced effective population sizes that are hallmarks of advanced animal societies. This is the first direct evidence of eusociality impacting genome evolution in marine taxa. Our results also strongly support the idea that eusociality can shape genome evolution through profound changes in life history and demography.
Frequent Assembly of Chimeric Complexes in the Protein Interaction Network of an Interspecies Yeast Hybrid
Authors: Dandage Rohan , Berger Caroline M , Gagnon-Arsenault Isabelle , Moon Kyung-Mee , Stacey Richard Greg , Foster Leonard J , Landry Christian R 
Published: November 2020

Abstract
Hybrids between species often show extreme phenotypes, including some that take place at the molecular level. In this study, we investigated the phenotypes of an interspecies diploid hybrid in terms of protein–protein interactions inferred from protein correlation profiling. We used two yeast species, Saccharomyces cerevisiae and Saccharomyces uvarum, which are interfertile, but yet have proteins diverged enough to be differentiated using mass spectrometry. Most of the protein–protein interactions are similar between hybrid and parents, and are consistent with the assembly of chimeric complexes, which we validated using an orthogonal approach for the prefoldin complex. We also identified instances of altered protein–protein interactions in the hybrid, for instance, in complexes related to proteostasis and in mitochondrial protein complexes. Overall, this study uncovers the likely frequent occurrence of chimeric protein complexes with few exceptions, which may result from incompatibilities or imbalances between the parental proteomes.
Parallel monitoring of RNA abundance, localization and compactness with correlative single molecule FISH on LR White embedded samples
Authors: Kramer Susanne , Meyer-Natus Elisabeth , Stigloher Christian , Thoma Hanna , Schnaufer Achim , Engstler Markus 
Published: November 2020

Abstract
Single mRNA molecules are frequently detected by single molecule fluorescence in situ hybridization (smFISH) using branched DNA technology. While providing strong and background-reduced signals, the method is inefficient in detecting mRNAs within dense structures, in monitoring mRNA compactness and in quantifying abundant mRNAs. To overcome these limitations, we have hybridized slices of high pressure frozen, freeze-substituted and LR White embedded cells (LR White smFISH). mRNA detection is physically restricted to the surface of the resin. This enables single molecule detection of RNAs with accuracy comparable to RNA sequencing, irrespective of their abundance, while at the same time providing spatial information on RNA localization that can be complemented with immunofluorescence and electron microscopy, as well as array tomography. Moreover, LR White embedding restricts the number of available probe pair recognition sites for each mRNA to a small subset. As a consequence, differences in signal intensities between RNA populations reflect differences in RNA structures, and we show that the method can be employed to determine mRNA compactness. We apply the method to answer some outstanding questions related to trans-splicing, RNA granules and mitochondrial RNA editing in single-cellular trypanosomes and we show an example of differential gene expression in the metazoan Caenorhabditis elegans.