bluuebunny/crossref_metadata_2025_split huggingface dataset:
Caminhos da educação infantil
Authors: Mello Ana Paula Barbieri de , Sudbrack Edite Maria 
Published: December 2019

Neste artigo, que é parte de dissertação de mestrado, vamos expor e discutir os principais pareceres e princípios dos documentos legais desde a Constituição Federal de 1988 até a BNCC para a Educação Infantil, com o intuito de compreender a contribuição de cada documento para as Políticas de Educação Infantil. Foi baseado nos princípios da metodologia histórico-crítica e foi delineado de forma teórica, bibliográfica e documental, com enfoque qualitativo. Elegemos o procedimento de análise de conteúdos para a descrição, compreensão e interpretação dos materiais encontrados. Os resultados revelam as contradições entre a legislação e a prática educacional, assim como levanta os aspectos históricos constituintes da temática abordada. Pelo contexto, conclui-se que as Políticas Públicas Educacionais para a Educação Infantil necessitam urgentemente de medidas pelas quais os políticos e gestores invistam recursos no âmbito educacional, tais como: amparo ao professor e os gestores com formação de qualidade (inicial e continuada), planejamento da infraestrutura de trabalho e dos materiais adequando-os à faixa etária, valorização profissional, com a presença de profissionais da educação na elaboração das leis e políticas públicas, entre outras ações. Isso significa deixar de lado as promessas, as incansáveis reformas e a descontinuidade de políticas públicas. E entendemos que a análise da BNCC é indispensável quando estudamos as políticas públicas de educação, pois é o documento mais recente da política curricular.
KEARIFAN LOKAL LINGKUNGAN KOMUNIKASI REMBUG DESA DALAM MENJAGA KELESTARIAN HUTAN DI KESATUAN PEMANGKUAN HUTAN (KPH) BANYUMAS TIMUR
Authors: Herutomo Ch. 
Published: December 2019

Salah satu kearifan lokal lingkungan dalam menjaga kelestarian hutan adalah komunikasi rembug desa yang dimiliki oleh masyarakat hutan khususnya di Pulau Jawa. Dalam komunikasi rembug desa sebagai lembaga demokrasi masyarakat desa hutan masyarakat punya kebebasan berbicara dengan spontan, humor tanpa distorsi dan keputusan diambil melalui musyawarah dan mufakat. Kearifan lokal sebagai nilai sosial budaya masyarakat desa hutan diwariskan secara turun temurun itu dapat membina hubungan yang harmonis anatara masyarakat desa hutan dengan hutan. Hubungan yang harmonis itu ditunjukkan dengan prilakunya sebagai penjaga hutan, mereka sadar bahwa hutan merupakan bagian dari ekosistem. Mereka akan hidup nyaman bila hutan aman sebaliknya akan hidup sengsara bila hutan mengalami kerusakan.
PENGARUH KREDIBILITAS YOUTUBER TERHADAP SIKAP PENONTON PADA CHANNEL YOUTUBE ATTA HALILINTAR (Studi Kuantitatif pada Penonton Youtube Atta Halilinar di Instagram @_ateam.ofc)
Authors: Fathiyyah Rahma Nur , Rina Nofha 
Published: December 2019

Saat ini, internet telah menjadi kebutuhan pokok bagi setiap masyarakat. Menurut survei The Manifest pada tahun 2018, disebutkan bahwa media sosial yang paling sering digunakan secara global adalah Youtube. Ini membuat youtuber, terutama di Indonesia, bersaing untuk mendapatkan keuntungan, baik manfaat materi dalam bentuk uang maupun manfaat non-materi seperti popularitas. Penelitian ini bertujuan untuk mengetahui dan menganalisis pengaruh kredibilitas youtuber terhadap sikap audiens. Variabel independen dalam penelitian ini adalah kredibilitas youtuber dengan dimensi kepercayaan, keahlian, dan daya tarik. Sedangkan variabel dependen dalam penelitian ini adalah sikap audiens dengan dimensi kognitif, afektif, dan konatif. Penelitian ini menggunakan metode kuantitatif. Penulis menentukan sampel menggunakan teknik probability sampling, dengan responden sebanyak 100 orang. Teknik analisis data yang digunakan oleh penulis adalah analisis data deskriptif, uji normalitas, uji regresi linier sederhana, koefisien determinasi dan uji hipotesis. Berdasarkan hasil pengujian hipotesis menggunakan uji-t, kredibilitas youtuber memiliki pengaruh terhadap sikap audiens. Ini dibuktikan dengan t-hitung (14,476)> ttabel (1,660). Berdasarkan koefisien determinasi, kredibilitas youtuber memiliki efek 68,1% pada sikap audiens.
The Effectiveness of Cananga Aromatherapy with Warm Compress in Reducing Premenstrual Syndrome (PMS) Amongst Teenage Girls
Authors: Ayu Nur Meity Sulistia 
Published: December 2019

<p align="center"><strong><em>Abstract</em></strong></p><p><em>Premenstrual Syndrome (PMS) appears 7-10 days before the menstrual period and can cause an uncomfortable feeling and pain with mild to severe symptoms that eventually can disturb the activities and quality of life of teenage girls. Prolonged use of chemical medication can cause several side effects. However, there is the option to use complementary therapy, such as Cananga aromatherapy with a warm compress, which is considered to be safer than chemical medication and has no side effects. The purpose of this research is to investigate the effectiveness of Cananga aromatherapy with a warm compress in reducing PMS in teenage girls. This research is a quasi experimental, pretest and posttest with control group design. Consecutive sampling was used to generate 68 respondents from teenage girls who were then divided into two groups - the intervention group that was given Cananga aromatherapy with a warm compress and the control group who did not receive the intervention. The bivariate analysis in the experimental group after the intervention, using the Cochran test, showed that the Cananga aromatherapy with a warm compress is effective in reducing PMS amongst teenage girls (p=0,000 &lt; ? = 0,05). Another analysis using the Kolmogorov-Smirnov test showed that </em>there is a difference in the level of PMS<em> between the experimental and the control group (p=0,002 &lt; ? = 0,05). It is therefore recommended that Cananga aromatherapy with a warm compress be used as a complementary therapy to reduce PMS amongst teenage girls.</em></p><p><em> </em></p><p><em>Key words: </em><em>Premenstrual Syndrome; PMS, Cananga Aromatherapy; Warm Compress; Teenage Girls</em></p>
Xylose and Arabinose Fermentation to Produce Ethanol by Isolated Yeasts from Durian (Durio zibethinus L.) Fruit
Authors: Hermansyah Hermansyah , Fachrijal Fachrijal , Miksusanti Miksusanti , Fatma Fatma , Kasmiarti Getari , Panagan Almunadi T 
Published: December 2019

Xylose and arabinose are pentosesugars that present in hemicellulose, part of lignocellulose biomass.These pentose sugars can be fermented by yeast into ethanol.The aim of this research was to utilize yeast isolated from durian fruit (DuriozibethinusL.) in fermentation of xylose and arabinose to produce bioethanol.Phenotypic test of isolates was conducted by growingthe isolates in various agar media, i.e.YPD (Yeast Peptone Dextrose), YPA (Yeast Peptone Arabinose), and YPX (Yeast Peptone Xylose) containing dextrose, arabinose, xylose, respectively, assole carbon source to see cell growth. The yeast isolates were further identified using API AOC 20C kit method. Yeast isolates were applied for fermentation of glucose, arabinose, and xylosein incubated cultures. Ethanol production in the fermentation was analyzed bygaschromatography. Yeast isolates were identified as Kodamaea ohmeri, Candida famata, Candida guilliermondii, and Crytococcuc laurentii. Based on gas chromatography data, it was found that ethanol produced in the fermentation for three days, the highest ethanol content on xylose substrate was fermented by Candida famata-Awhich is0.021% (v/v) ethanol resulted from initial concentration of 5% xylose (w/v). While on arabinose substrate, the highest ethanol content was fermented by Crytococcus laurentii-Bwhich is 0.0034% (v/v) ethanol resulted from initial concentration of 5% arabinose (w/v).
OXYGEN ISOTOPE PROFILES OF UPPERMOST JURASSIC VERTEBRATE TEETH AND OYSTER SHELLS: A RECORD OF PALEOENVIRONMENTAL CHANGES AND ANIMAL HABITATS
Authors: WIERZBOWSKI HUBERT , BŁAŻEJOWSKI BŁAŻEJ , TYBOROWSKI DANIEL 
Published: December 2019

ABSTRACTHigh resolution oxygen isotopes profiles of well-preserved uppermost Jurassic oyster shells and vertebrate (ichthyosaur and fish) teeth from Central Poland were obtained using a microdrill and an ion microprobe (SHRIMP IIe/MC). Internal variability of δ18O values of oyster shells (-1.7 to 1.3‰ VPDB) collected from the offshore interval of the studied section is greater than that determined previously from bulk oysters. Relatively high δ18O values of the studied oysters may be linked to a slight increase in the salinity of the mid-Polish basin. Low δ18O values of co-occurring ichthyosaur tooth enamel substantiate endothermy of these reptiles. An observed ontogenetic increase in the ichthyosaur enamel δ18O values (from ∼ 19.4 to ∼ 21.6‰ VSMOW) may be a result of long distance migrations although the effect of metabolic factors at rising body mass cannot be completely excluded. It is postulated that δ18O values of the latest portion of the ichthyosaur tooth enamel can be used for the calculation of ancient water oxygen isotope composition and verification of oxygen isotope temperatures. High δ18O values of the enameloid of Caturus lungfishes (23.6 to 26.5‰ VSMOW) collected from an upper part of the studied section, which was deposited in a restricted lagoonal environment after a marine regression, are probably a result of a high evaporation rate. The fishes could have breathed atmospheric air and withstood episodes of water hypoxia. Internal oxygen isotope records of the shells and teeth are promising proxies for studies of the paleoenvironment and activity of animals.
Complete genome sequence analysis of a novel granaticin producer, Streptomyces sp. A1013Y
Authors: Zheng Shenglan , Zhu Yunping , Liu Kaifeng , Li Jinlong 
Published: December 2019

Abstract
Granaticin is a kind of antibiotics with the function of anticancer, antibacterial, anticoccidial. Streptomyces sp. A1013Y is a new strain isolated from soil with granaticin-producing ability. The genome information was analyzed for the further study of granaticin. The present work reported the complete genome of S. sp. A1013Y, which contains a 7,646,296 bp chromosome with an average GC content of 71.59%. A total of 7361 CDSs, including 66 tRNA genes, 18 rRNA genes and 23 clusters, were identified in the genome. With homologous recombination, an in-frame deletion mutant was constructed to confirm the granaticin cluster. The fermentation liquid of the mutant did not contain granaticin based on HPLC and LC-MS analysis, indicating that S. sp. A1013Y is a granaticin producer. Comparing the granaticin biosynthesis cluster of S. sp. A1013Y with Streptomycesviolaceoruber Tü22 and Streptomyces vietnamensis GIMV4.0001T, which were sequenced and produce granaticin, the similarity between the granaticin clusters was 78% and 83% respectively, but some genes still weren’t identified in the granaticin biosynthesis cluster. The phylogenetic analysis and ANI value between S. sp. A1013Y and other Streptomyces species, including S. vietnamensis GIMV4.0001T, were all below 85%, which showed that S. sp. A1013Y was probably a novel streptomyces strain that produced granaticin. The whole genome information of S. sp. A1013Y provides a valuable foundation for future granaticin analyses as well as biomedicine applications.
Multi-drug resistant, extended spectrum beta-lactamase and carbapenemase producing bacterial isolates among children under five years old with suspected bloodstream infection in a specialized hospital in Ethiopia: Cross-sectional study
Authors: Tadesse Mequanint Mitiku , Matebie Zeleke Ayenew , Tullu Kassu Desta 
Published: December 2019

Abstract
BackgroundBloodstream infections due to bacterial pathogens are a major cause of morbidity and mortality among pediatric patients. Emergence of drug resistance in high classes to antibiotics among the bacterial pathogens is another issue of public health concern. Therefore, this study was conducted to determine multi-drug resistant, extended spectrum β-lactamase and carbapenemase producing bacterial isolates among suspected bloodsteam infection patients in children under five years of age at Tikur Anbessa Specialized Hospital, Addis Ababa, Ethiopia.MethodsA cross-sectional study was conducted from September 2017 to June 2018 among pediatric patients with febrile illness under five years of age at Tikur Anbessa Specialized Hospital. Three hundred and forty blood samples were collected and processed following standard microbiological techniques and blood culture was performed using a BacT/Alert instrument in combination with conventional methods for identification. Antimicrobial susceptibility testing of the isolates was performed using the Kirby-Bauer disc diffusion method to determine the minimum inhibitory concentration (MIC).ResultA total of 137 (40.2%) bacterial pathogens were isolated from 340 pediatric patients suspected of bloodstream infection with febrile illness. Of these isolates, 54% were Gram negative bacteria. Among gram negative isolates 43 (31.4%) were identified as Klebsiella pneumoniae and 8.7% Acinetobacter species were the most frequently isolated pathogens. Klebsiella pneumoniae isolates were 88.4 % (38/43) MDR, 32.5% (14/43) ESBL, and 37.2 %( 16/43) CRE .ConclusionIn this study, highly resistant Klebsiella pneumoniae are common pathogen associated with BSI. Extended spectrum beta-lactamase (ESBL) producing strains were common in Klebsiella species and Escherichia coli isolates. Since most of isolates exhibit multidrug resistance, in vitro antimicrobial susceptibility testing is mandatory. A strengthened antimicrobial surveillance system and antimicrobial stewardship programs are necessary for better selection of antibiotics in addition to improved infection prevention practices in hospital settings.
A Study of 0-14-Year-Old Children’s Access to Health Centers in Rural Areas Using a Buffer Model (a Case Study of Villages Based in Kermanshah Province, Iran)
Authors: Almasi Ali , Zangeneh Alireza , Saeidi Shahram , Ziapour Arash , Teimouri Raziyeh , Mahaki Behzad 
Published: December 2019

Abstract
Background: Children are among the most vulnerable groups in society, whose health is of prominent significance. Moreover, as a group of clients with special needs in the health care system, they require special attention. Therefore, the present study aimed to investigate the 0-14-year-old children’s access to health centers in rural areas of Kermanshah Province, Iran. Methods: In the present cross-sectional study, both the latest published demographic statistics related to the Population and Housing Census, announced by the Statistical Center of Iran in 2011, and the information about the public and private hospitals in the province, collected by Kermanshah University of Medical Sciences, were used as the basis for the analyses. Additionally, given the importance of spatial nature of the research, GIS was used for data analysis, and a buffer model was also applied. Results: The results revealed that out of the total population of 0-14-year-old children residing in rural areas within 15,000 and 30,000-Kilometer radii of Kermanshah Province, 87.94% and 75.11% of girls versus 88.15% and 75.38% of boys lacked access to health centers, respectively. Conclusion: It was found out that the 0-14-year-old children’s access to health centers was in poor condition in rural areas of Kermanshah Province, which would endanger the health of this age group.
Age as a decisive factor in general anesthesia use in pediatric proton beam therapy.
Authors: SHIMAZU YUZO , Otsuki Rie , Murakani Masao , Konishi Akio , Kan Keiichi , Seto Ichiro , Yamaguchi Hisahi , Tsubokura Masaharu , Hattori Hisashi 
Published: December 2019

Abstract
Background: Proton therapy for pediatric cancer patients is effective treatments. Young children have difficulties staying still during irradiation, so general anesthesia is often required. This study investigated the indication for general anesthesia in pediatric proton therapy.Methods: We focused on cancer patients under 15 years old who underwent proton therapy at Southern TOHOKU General Hospital, Fukushima, Japan from April 2016 to December 2018. Background information and anesthesia/treatment protocols were retrospectively extracted from the electronic medical record. The anesthesia and non-anesthesia group were compared to evaluate factors determining the need for general anesthesia .Results: We analyzed 32 patients who received 285 irradiations. The median age was 5 years old (range: 1–15), and 13 patients (40.6%) were female. Twelve (37.5%) patients were treated with general anesthesia. In the general anesthesia group, airway management using laryngeal mask was performed in 11 patients (91.6%). Age was significantly lower in the general anesthesia group than in the non-anesthetized group (p < 0.001). Considering all background factors, only age was strongly associated with anesthesia in the univariate logistic regression models: odds ratio 0.55 [95% confidence interval 0.35–0.86] (P < 0.01). Adverse events included nausea and vomiting during and after irradiation, and no fatal complications were observed.Conclusion: Age is one of the most important factors determining the need for general anesthesia for PBT in children. it will be necessary to determine the guidelines for accumulation and collection of data between institutions and the indication of general anesthesia/sedation for radiation therapy including PBT.
Physical activity level and its demographic and psychosocial correlates among school-going adolescents in Obollo-Afor Education Zone, south-east Nigeria: a cross-sectional study
Authors: Agbaje Olaoluwa Samson , Umoke Prince Iheanachor Christian , Attama Charity Chika , Ugwueze Fabian Chibunine , Agu Benedicta Ndidi , Ozoemena Eyuche Lawretta , Kato George U. , Obute Akor J. 
Published: December 2019

Abstract
Background: Physical inactivity (PA) is a modifiable risk factor for many chronic illnesses. Studies on physical activity in adolescent populationshave reported associations between PA and demographic and psychosocial factors. However, there is a limited evidence on PA level and associated factors among adolescents in south-east Nigeria. Thus, the study examined adolescents’ PA level and its demographic and psychosocial correlates among school-going adolescents in Obollo-Afor Education Zone, Enugu State, South-East Nigeria.Methods: A total of 365 randomly selected adolescents aged 12-19 years in Obollo-Afor, Enugu State, south-East Nigeria participated in the study. The Physical Activity Questionnaire for Adolescents (PAQ-A), the Physical Self-Description Questionnaire-Short Form (PSDQ-S), the Social Support and Peer Norms Scales for Physical Activity (SSPNS)were used to measure PA level, physical self-concept, social support and peer norms for PA.Data were analyzed using descriptive statistics, independent samples t -test, fixed effects ANOVA, and multiple linear regression.Results: The mean age of the participants was 15.76 ± 1.78 years for boys and 15.65 ± 1.83 years for girls. One-third (33.3%) had low PA level, more than half of the participants had moderate PA level(58.1%) whilevery few (8.5%) had high PA level. School-going adolescents had high mean physical self-concept(16.6 ± 40), social support(2.48 ± 1.04) and peer norms(2.19 ± 1.049). Adolescents’ PA level was positively related to self-concept ( r = .06, p > 0.05), social support ( r = .26, p < 0.01), peer norms ( r = .27, p < 0.01). Social support (beta = .22, p < .001), peer norms received for PA (beta = .23, p < .003), and class of study (beta = -.047, p < 0.05) were significantly associated with PA level among adolescents.Conclusions: One third of school-going adolescents had low level of PA, and low proportion had high level of PA. Also, Social support, peer norms received for PA and class of study were significantly associated with PA among adolescents. Future studies are needed to design interventions that promote PA among adolescents in Nigeria.
Coupling structural characterization with secretomic analysis reveals mechanism of the disruption of cross-linked structure in bamboo culms by Echinodontium taxodii
Authors: Kong Wen , Zhang Jialong , Xiao Qiuyun , Liu Jiashu , Cao Zhixiang , Ma Fuying , Yu Hongbo 
Published: December 2019

Abstract
Background: Overcoming the biomass recalcitrance is essential for efficient utilization of lignocellulosic biomass in industrial bio-refining. White-rot fungi can overcome the biomass recalcitrance and accelerate the conversion of lignocellulose to biofuels via a large number of special extracellular lignocellulolytic enzymes. Previous studies try to dissect the function of extracellular enzymes on biomass resistant cross-linked structures by secretome analysis, but the bio-alteration of cross-linked structures is ignored usually. A deeper and detailed understanding of relationship between secretome and bio-alteration of cross-linked structure in lignocellulosic biomass is still lack. Results: As an efficient wood-decaying fungus, Echinodontium taxodii could improve the conversion efficiency of lignocellulose to biofuels. This study coupled comparative analysis of fungal secretomes and 2D HSQC NMR analysis of lignocellulose fractions, aiming to elucidate the role of extracellular enzymes from Echinodontium taxodii 2538 in the disruption of resistant cross-linked structure of bamboo culms. Carboxylesterases, alcohol oxidases and Class-II peroxidases showed importance in the cleavage of cross-linked structures, including ester and ether linkages of lignin-carbohydrate complexes (LCCs) and inter-unit linkages of lignin, which contributed to biomass resistance removal and cellulose exposure during the early stage of fungal decay. Moreover, the rapid oxidation of Cα-OH was found to contribute to the lignin bio-depolymerization. Conclusions: These findings revealed the detailed mechanisms of biomass recalcitrance reduction by fungal pretreatment, and provide insight into efficient strategy of lignocellulose conversion. It will advance the development in design of enzyme cocktail for efficient lignocellulose bio-refinery.
Reversed paired-gRNA plasmid cloning strategy for efficient genome editing in Escherichia coli
Authors: Ding Tingting , Huang Chaoyong , Liang Zeyu , Ma Xiaoyan , Wang Ning , Huo Yi-Xin 
Published: December 2019

Abstract
BackgroundThe CRISPR-Cas9 system is a powerful tool for genome editing in various organisms. Several of its applications, including the generation of large deletions, require co-expression of two distinct guide RNAs (gRNAs). However, the instability of paired-gRNA plasmids prevents these applications from being scalable in Escherichia coli. Coexpressing paired gRNAs under the driving of independent but identical promoters in the same direction triggers plasmid recombination, due to the presence of direct repeats (DRs). ResultsIn this study, plasmid deletion between DRs occurred with high frequencies during plasmid construction and subsequent duplication processes, when three DRs-involved paired-gRNA plasmids cloning strategies were tested. This recombination phenomenon was RecA-independent, in agreement with the replication slippage model. To completely eliminate the DRs-induced plasmid instability, a reversed paired-gRNA plasmids (RPGPs) cloning strategy was developed by converting DRs to the more stable invert repeats (IRs). ConclusionsUsing RPGPs, we achieved a rapid deletion of chromosome fragments up to 100 kb with high efficiency of 83.33% in Escherichia coli. This study provides general solutions to construct stable plasmids containing short DRs, which can improve the performances of CRISPR systems that rely on paired gRNAs, and also facilitate other applications involving repeated genetic parts.