bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impact of Personalized Genetic Breast Cancer Risk Estimation With Polygenic Risk Scores on Preventive Endocrine Therapy Intention and Uptake
Authors: Kim Julian O. , Schaid Daniel J. , Vachon Celine M. , Cooke Andrew , Couch Fergus J. , Kim Christina A. , Sinnwell Jason P. , Hasadsri Linda , Stan Daniela L. , Goldenberg Benjamin , Neal Lonzetta , Grenier Debjani , Degnim Amy C. , Thicke Lori A. , Pruthi Sandhya 
Published: October 2020

AbstractEndocrine therapy is underutilized to reduce breast cancer incidence among women at increased risk. Polygenic risk scores (PRSs) assessing 77 breast cancer genetic susceptibility loci personalizes risk estimates. We examined effect of personalized PRS breast cancer risk prediction on intention to take and endocrine therapy uptake among women at increased risk. Eligible participants had a 10-year breast cancer risk ≥5% by Tyrer–Cuzick model [International Breast Cancer Intervention Study (IBIS)] or ≥3.0 % 5-year Gail Model risk with no breast cancer history or hereditary breast cancer syndrome. Breast cancer risk was estimated, endocrine therapy options were discussed, and endocrine therapy intent was assessed at baseline. After genotyping, PRS-updated breast cancer risk estimates, endocrine therapy options, and intent to take endocrine therapy were reassessed; endocrine therapy uptake was assessed during follow-up. From March 2016 to October 2017, 151 patients were enrolled [median (range) age, 56.1 (36.0–76.4 years)]. Median 10-year and lifetime IBIS risks were 7.9% and 25.3%. Inclusion of PRS increased lifetime IBIS breast cancer risk estimates for 81 patients (53.6%) and reduced risk for 70 (46.4%). Of participants with increased breast cancer risk by PRS, 39 (41.9%) had greater intent to take endocrine therapy; of those with decreased breast cancer risk by PRS, 28 (46.7%) had less intent to take endocrine therapy (P &lt; 0.001). On multivariable regression, increased breast cancer risk by PRS was associated with greater intent to take endocrine therapy (P &lt; 0.001). Endocrine therapy uptake was greater among participants with increased breast cancer risk by PRS (53.4%) than with decreased risk (20.9%; P &lt; 0.001). PRS testing influenced intent to take and endocrine therapy uptake. Assessing PRS effect on endocrine therapy adherence is needed.Prevention Relevance: Counseling women at increased breast cancer risk using polygenic risk score (PRS) risk estimates can significantly impact preventive endocrine therapy uptake. Further development of PRS testing to personalize breast cancer risk assessments and endocrine therapy counselling may serve to potentially reduce the incidence of breast cancer in the future.
Incidence and Outcomes of Out‐of‐Hospital Cardiac Arrest in Singapore and Victoria: A Collaborative Study
Authors: Lim Shir Lynn , Smith Karen , Dyson Kylie , Chan Siew Pang , Earnest Arul , Nair Resmi , Bernard Stephen , Leong Benjamin Sieu‐Hon , Arulanandam Shalini , Ng Yih Yng , Ong Marcus Eng Hock 
Published: October 2020

Background
Incidence and outcomes of out‐of‐hospital cardiac arrest (OHCA) vary between communities. We aimed to examine differences in patient characteristics, prehospital care, and outcomes in Singapore and Victoria.

Methods and Results

Using the prospective Singapore Pan‐Asian Resuscitation Outcomes Study and Victorian Ambulance Cardiac Arrest Registry, we identified 11 061 and 32 003 emergency medical services‐attended adult OHCAs between 2011 and 2016 respectively. Incidence and survival rates were directly age adjusted using the World Health Organization population. Survival was analyzed with logistic regression, with model selection via backward elimination. Of the 11 061 and 14 834 emergency medical services‐treated OHCAs (overall mean age±SD 65.5±17.2; 67.4% males) in Singapore and Victoria respectively, 11 054 (99.9%) and 5595 (37.7%) were transported, and 440 (4.0%) and 2009 (13.6%) survived. Compared with Victoria, people with OHCA in Singapore were older (66.7±16.5 versus 64.6±17.7), had less shockable rhythms (17.7% versus 30.3%), and received less bystander cardiopulmonary resuscitation (45.7% versus 58.5%) and defibrillation (1.3% versus 2.5%) (all
P
<0.001). Age‐adjusted OHCA incidence and survival rates increased in Singapore between 2011 and 2016 (
P
<0.01 for trend), but remained stable, though higher, in Victoria. Likelihood of survival increased significantly (
P
<0.001) with arrest in public locations (adjusted odds ratio [aOR] 1.81), witnessed arrest (aOR 2.14), bystander cardiopulmonary resuscitation (aOR 1.72), initial shockable rhythm (aOR 9.82), and bystander defibrillation (aOR 2.04) but decreased with increasing age (aOR 0.98) and emergency medical services response time (aOR 0.91).

Conclusions
Singapore reported increasing OHCA incidence and survival rates between 2011 and 2016, compared with stable, albeit higher, rates in Victoria. Survival differences might be related to different emergency medical services practices including patient selection for resuscitation and transport.
The Parameter Dependence of Eddy Heat Flux in a Homogeneous Quasigeostrophic Two-Layer Model on a β Plane with Quadratic Friction
Authors: Chang Chiung-Yin , Held Isaac M. 
Published: October 2020

Abstract
This study investigates the parameter dependence of eddy heat flux in a homogeneous quasigeostrophic two-layer model on a β plane with imposed environmental vertical wind shear and quadratic frictional drag. We examine the extent to which the results can be explained by a recently proposed diffusivity theory for passive tracers in two-dimensional turbulence. To account for the differences between two-layer and two-dimensional models, we modify the two-dimensional theory according to our two-layer f-plane analyses reported in an earlier study. Specifically, we replace the classic Kolmogorovian spectral slope, −5/3, assumed to predict eddy kinetic energy spectrum in the former with a larger slope, −7/3, suggested by a heuristic argument and fit to the model results in the latter. It is found that the modified theory provides a reasonable estimate within the regime where both

and the strength of the frictional drag,

, are much smaller than unity (here, c

D
is the nondimensional drag coefficient divided by the depth of the layer, k

d
is the wavenumber of deformation radius, and U is the imposed background vertical wind shear). For values of

and

that are closer to one, the theory works only if the full spectrum shape of the eddy kinetic energy is given. Despite the qualitative, fitting nature of this approach and its failure to explain the full parameter range, we believe its documentation here remains useful as a reference for the future attempt in pursuing a better theory.
Regularity and Irregularity of the Seasonal Northward March of the East Asian Summer Wet Environment and the Influential Factors
Authors: Sun Bo , Wang Huijun , Wang Aihui , Miao Yue , Zhou Botao , Li Huixin 
Published: October 2020

AbstractThis study investigated the characteristics of both the regular and irregular seasonal northward march of the East Asian summer wet environment (SNM_EASWE) over the continent during June–July over the past six decades and in the future. During 1961–2015 and in the future, the regular SNM_EASWE is defined mainly by an intensified climatic northward-moving rainband over eastern China, whereas the irregular SNM_EASWE is defined mainly by less clear features of northward-moving rainband. Further analyses indicate that the boreal summer intraseasonal oscillation (BSISO) played a dominant role in affecting the regular/irregular SNM_EASWE during 1961–79, whereas both the BSISO and the seasonally varying western North Pacific (WNP) eddy may exert notable influences on the regular/irregular SNM_EASWE during 1980–2015 and in the future, where the WNP eddy refers to the anomalous anticyclone/cyclone over the WNP associated with the anomalous western Pacific subtropical high. During 1980–2015, a La Niña–like (El Niño–like) condition in the tropical Pacific during June–July may induce a regular (irregular) SNM_EASWE case because of the influence of anomalous convection over the Maritime Continent on the seasonally varying WNP eddy; an anomalous warming (cooling) in the tropical Indian Ocean during June–July may also partly induce a regular (irregular) SNM_EASWE case via influencing the seasonally varying WNP eddy. The increased influence of the seasonally varying WNP eddy on the regular/irregular SNM_EASWE after 1979 is attributed to the increased interannual variability of convective activity over the Maritime Continent and of sea surface temperatures in the Arabian Sea after 1979.
Evaluation of a Care Management Program for Pediatric Epilepsy Patients
Authors: Min Jea Young , Patel Anup D. , Glynn Peter , Otgonsuren Munkhzul , Harridas Babitha , Grinspan Zachary M. 
Published: October 2020

Objective: To evaluate the impact of a pediatric epilepsy care management intervention on emergency department visits, hospitalizations, and seizure freedom. Methods: We conducted a prospective observational study at a single academic medical center. Children with epilepsy with high risk of frequent emergency department use were enrolled in the intervention from January through May 2015, which included a baseline visit and follow-up support from a care management team. Controls selected from the same institution received standard of care. Baseline and follow-up information were collected from electronic health records and surveys (Family Impact Scale, Pediatric Epilepsy Medication Self-Management Questionnaire). Propensity score–weighted logistic regression compared emergency department visits, unplanned hospitalizations, and 3-month seizure freedom after 1 year in the intervention vs control groups. Results: A total of 56 children were enrolled in the intervention and 359 received standard of care. The intervention group was younger and had greater use of health services at baseline. When comparing the intervention to standard of care after 1 year, we found no significant difference in the risk of any emergency department visit (adjusted odds ratio [OR] 2.2, 95% confidence interval [CI] 0.6-8.5) or seizure freedom (adjusted OR 2.5, 95% CI 0.3-21.5). However, the risk of unplanned hospital admissions remained higher in the intervention group (adjusted OR 23.1, 95% CI 5.1-104). Conclusion: We did not find that children with epilepsy who received a care management intervention had less use of health services or better clinical outcomes after a year compared with controls. The study is limited by small sample size and nonrandomized study design.
High-quality genome sequence assembly of R.A73 Enterococcus faecium isolated from freshwater fish mucus
Authors: El Jeni Rim , Ghedira Kais , El Bour Monia , Abdelhak Sonia , Benkahla Alia , Bouhaouala-Zahar Balkiss 
Published: October 2020

Abstract
Background
Whole-genome sequencing using high throughput technologies has revolutionized and speeded up the scientific investigation of bacterial genetics, biochemistry, and molecular biology. Lactic acid bacteria (LABs) have been extensively used in fermentation and more recently as probiotics in food products that promote health. Genome sequencing and functional genomics investigations of LABs varieties provide rapid and important information about their diversity and their evolution, revealing a significant molecular basis.
This study investigated the whole genome sequences of the Enterococcus faecium strain (HG937697), isolated from the mucus of freshwater fish in Tunisian dams. Genomic DNA was extracted using the Quick-GDNA kit and sequenced using the Illumina HiSeq2500 system. Sequences quality assessment was performed using FastQC software. The complete genome annotation was carried out with the Rapid Annotation using Subsystem Technology (RAST) web server then NCBI PGAAP.

Results
The Enterococcus faecium R.A73 assembled in 28 contigs consisting of 2,935,283 bps. The genome annotation revealed 2884 genes in total including 2834 coding sequences and 50 RNAs containing 3 rRNAs (one rRNA 16 s, one rRNA 23 s and one rRNA 5 s) and 47 tRNAs. Twenty-two genes implicated in bacteriocin production are identified within the Enterococcus faecium R.A73 strain.

Conclusion
Data obtained provide insights to further investigate the effective strategy for testing this Enterococcus faecium R.A73 strain in the industrial manufacturing process. Studying their metabolism with bioinformatics tools represents the future challenge and contribution to improving the utilization of the multi-purpose bacteria in food.
Alternative splicing profiling provides insights into the molecular mechanisms of peanut peg development
Authors: Zhao Xiaobo , Li Chunjuan , Zhang Hao , Yan Caixia , Sun Quanxi , Wang Juan , Yuan Cuiling , Shan Shihua 
Published: October 2020

Abstract

Background
The cultivated peanut (Arachis hypogaea) is one of the most important oilseed crops worldwide, and the generation of pegs and formation of subterranean pods are essential processes in peanut reproductive development. However, little information has been reported about alternative splicing (AS) in peanut peg formation and development.

Results
Herein, we presented a comprehensive full-length (FL) transcriptome profiling of AS isoforms during peanut peg and early pod development. We identified 1448, 1102, 832, and 902 specific spliced transcripts in aerial pegs, subterranean pegs, subterranean unswollen pegs, and early swelling pods, respectively. A total of 184 spliced transcripts related to gravity stimulation, light and mechanical response, hormone mediated signaling pathways, and calcium-dependent proteins were identified as possibly involved in peanut peg development. For aerial pegs, spliced transcripts we got were mainly involved in gravity stimulation and cell wall morphogenetic processes. The genes undergoing AS in subterranean peg were possibly involved in gravity stimulation, cell wall morphogenetic processes, and abiotic response. For subterranean unswollen pegs, spliced transcripts were predominantly related to the embryo development and root formation. The genes undergoing splice in early swelling pods were mainly related to ovule development, root hair cells enlargement, root apex division, and seed germination.

Conclusion
This study provides evidence that multiple genes are related to gravity stimulation, light and mechanical response, hormone mediated signaling pathways, and calcium-dependent proteins undergoing AS express development-specific spliced isoforms or exhibit an obvious isoform switch during the peanut peg development. AS isoforms in subterranean pegs and pods provides valuable sources to further understand post-transcriptional regulatory mechanisms of AS in the generation of pegs and formation of subterranean pods.
Circular RNA hsa_circ_0000282 contributes to osteosarcoma cell proliferation by regulating miR-192/XIAP axis
Authors: Li Houkun , He Limin , Tuo Yuan , Huang Yansheng , Qian Bing 
Published: October 2020

Abstract
Background
Circular RNAs (circRNAs) have emerged as a novel category of non-coding RNA, which exhibit a pivotal effect on regulating gene expression and biological functions, yet how circRNAs function in osteosarcoma (OSA) still demands further investigation. This study aimed at probing into the function of hsa_circ_0000282 in OSA.

Methods
The expressions of circ_0000282 and miR-192 in OSA tissues and cell lines were examined by quantitative real-time polymerase chain reaction (qRT-PCR), and the correlation between the expression level of circ_0000282 and clinicopathological features of OSA patients was analyzed. The expressions of X-linked inhibitor of apoptosis protein (XIAP), B-cell lymphoma-2 (Bcl-2) and Bcl-2 associated X protein (Bax) in OSA cells were assayed by Western blot. The proliferation and apoptosis of OSA cells were examined by CCK-8, BrdU and flow cytometry, respectively. Bioinformatics analysis, dual-luciferase reporter gene assay and RIP experiments were employed to predict and validate the targeting relationships between circ_0000282 and miR-192, and between miR-192 and XIAP, respectively.

Results
Circ_0000282 was highly expressed in OSA tissues and cell lines, which represented positive correlation with Enneking stage of OSA patients and negative correlation with tumor differentiation degree. In vitro experiments confirmed that overexpression of circ_0000282 markedly facilitated OSA cell proliferation and repressed cancer cell apoptosis in comparison to control group. Besides, knockdown of circ_0000282 repressed OSA cell proliferation and promoted apoptosis. Additionally, the binding relationships between circ_0000282 and miR-192, and between miR-192 and XIAP were validated. Circ_0000282 indirectly up-regulated XIAP expression by adsorbing miR-192, thereby playing a role in promoting cancer in OSA.

Conclusion
Circ_0000282 was a novel oncogenic circRNA in OSA. Circ_0000282/miR-192/XIAP axis regulated OSA cell proliferation apoptosis with competitive endogenous RNA mechanism.
De Clérambault’s syndrome revisited: a case report of Erotomania in a male
Authors: Valadas Maria Teresa Tavares Rodrigues Toma , Bravo Lucilia Eduarda Abrantes 
Published: October 2020

Abstract
Background
Erotomania, also known as “de Clérambault’s Syndrome”, is a psychiatric syndrome characterized by the delusional belief that one is loved by another person of, generally of a higher social status. Erotomania has always been a target of attempts of conceptualization, and the utility of regarding Erotomania as an independent syndrome has been questioned to this day. Erotomania has a much higher prevalence in the female sex, and male Erotomania is a rare and probably underdiagnosed condition. Male Erotomania is only more prevalent in forensic samples, since male sex is one of the risk factors for violent behavior in this disorder. In this article, we aim to describe an uncommon case of Erotomania occurring in a male, hoping to add to literature and to reflect on the implications of the occurrence of Erotomania in men. By discussing the case in light of the different described clinical pictures, proposed diagnostic criteria and classifications, we also aim to contribute to the ongoing attempt to conceptualize this syndrome and to understand the pertinence of considering it an independent nosological entity.

Case presentation
We describe a case of Erotomania in a middle-aged Caucasian Portuguese male, with consecutive erotic delusions, followed by a classic turn to a persecutory delusion. The patient was admitted as an inpatient in a psychiatry unit and he was medicated with risperidone 3 mg and diazepam 3 mg daily. His persecutory delusion remitted a 4 days later, he gained insight and was discharged to follow-up as an outpatient. He retains his erotic delusional beliefs, but these are less intense, and has not presented further aggressive behavior.

Conclusions
We can conclude that it seems reasonable to retain the operative concept of Erotomania as a subtype of Persistent Delusional Disorder/Delusional Disorder, since cases fitting the classical descriptions of the syndrome have been reported, including the presented case. The diagnosis of Erotomania has implications in case management, treatment and prognosis, and missing it, especially in men, may culminate in violent situations that can have legal implications. The developed diagnostic criteria and classifications seem to retain use and should be kept in mind, in the sense that they facilitate making an important diagnosis.
“My work? Well, I live it and breathe it”: The seamless connect between the professional and personal/community self in the Aboriginal and Torres Strait Islander health sector
Authors: Dickson Michelle 
Published: October 2020

AbstractBackgroundAustralian Aboriginal and Torres Strait Islander health professionals often juggle the challenges of working and living in the same community in ways that are positive for both themselves and their clients. This study specifically examines the strategies Aboriginal and Torres Strait Islander health professionals have developed to enable them to feel empowered by the sense of being always visible or perceived as being always available. Findings provide examples of how participants (Team Members) established a seamless working self, including how they often held different perspectives to many work colleagues, how Team Members were always visible to community and how Team Members were comfortable to be seen as working when not at work.MethodsThis qualitative study engages an Indigenous research methodology and uses an Indigenous method, PhotoYarning, to explore lived experiences of a group (n = 15) of Aboriginal and Torres Strait Islander health workers as they worked in the Australian health sector.ResultsThe analysis presented here comes from data generated through PhotoYarning sessions. Team Members in this study all work in health care settings in the communities in which they also live, they manage an extremely complex network of interactions and relationships in their daily working lives. They occupy an ambivalent, and sometimes ambiguous, position as representing both their health profession and their community. This article explores examples of what working with seamlessness involved, with findings citing four main themes: (1) Being fellow members of their cultural community, (2) the feeling of always being visible to community as a health worker, (3) the feeling of always being available as a health worker to community even when not at work and (4) the need to set an example.ConclusionsWhile creating the seamlessness of working and living in the same community was not easy, Team Members considered it an important feature of the work they did and vital if they were to be able to provide quality health service to their community. However, they reported that the seamless working self was at odds with the way many of their non-Indigenous Australian colleagues worked and it was not well understood.
Evaluation of an isothermal amplification HPV detection assay for primary cervical cancer screening
Authors: Zhang Wei , Du Hui , Huang Xia , Wang Chun , Duan Xianzhi , Liu Yan , Shi Bin , Zhang Wei , Qu Xinfeng , Wei Lihui , Schiffman M. , Belinson J. L. , Wu Ruifang 
Published: October 2020

Abstract
Objective
The aim of this research was to evaluate independently the performance of a new isothermal amplification assay for cervical cancer screening compared to two previously validated PCR-based assays and histologic endpoints.

Methods
This is a sub-study from the Chinese multi-center screening trial (CHIMUST). The self-collected and clinician-collected specimens stored in PreservCyt at − 4 °C from 6042 women with complete data were tested with the AmpFire assay. These specimens had been previously tested with Cobas and SeqHPV assays. In the primary study all patients with an abnormal test were referred to colposcopy where all had directed and/or random biopsies plus ECC. No additional patients were called back based on the AmpFire results.

Results
6042/6619 women had complete data (mean age 44.1). There were 57 cases of CIN 2, 35 cases of CIN 3 and 2 cancers. The sensitivity for CIN2+ and CIN3+ were similar among the three assays (both direct and self-collected). For the specificities in all categories (CIN2+/CIN3+ and self and direct collection), isothermal amplification assay was either equal to or more specific than Cobas but consistently less specific than SeqHPV.

Conclusion
The AmpFire HPV assay showed similar sensitivity to Cobas and SeqHPV for CIN2+ and CIN3+ on both self and clinician-collections (P>0.05), with good specificity. The speed, low cost, and simplicity of this assay will make it particularly suited for low and middle resource settings. Its accuracy with self-collection makes it applicable for mass screening programs.