bluuebunny/crossref_metadata_2025_split huggingface dataset:
The role of the meningeal lymphatic system in local inflammation and trigeminal nociception implicated in migraine pain
Authors: Mikhailov Nikita , Koroleva Kseniia , Abdollahzadeh Ali , Giniatullina Raisa , Gafurov Oleg , Malm Tarja , Sierra Alejandra , Tohka Jussi , Noe Francesco , Giniatullin Rashid 
Published: April 2020

AbstractBackgroundA system of lymphatic vessels has been recently characterized in the meninges, with a postulated role in ‘cleaning’ the brain via cerebral fluid drainage. As meninges are the origin site of migraine pain, we hypothesized that malfunctioning of the lymphatic system should affect the local trigeminal nociception. To test this hypothesis, we studied nociceptive and inflammatory mechanisms in the meninges of K14-VEGFR3-Ig mice lacking the meningeal lymphatic system.MethodsWe recorded the spiking activity of meningeal afferents and estimated the local mast cells infiltration, calcitonin gene-related peptide (CGRP) and cytokine levels (basal and stimulated), as well as the dural trigeminal innervation in freshly-isolated hemiskull preparations from K14-VEGFR3-Ig (K14) or wild type C57BL/6 mice (WT).ResultsWe found that the meningeal level of CGRP and of the pro-inflammatory cytokines IL12-p70 and TNFα (implicated in migraine) were reduced in the meninges of K14 mice. On the contrary, in the meninges of K14 mice, we found an increased level of the mast cell activator MCP-1 and, consistently, a larger number of dural mast cells. The other migraine-related pro-inflammatory cytokines did not differ between the two genotypes. The patterns of trigeminal innervation in meninges remained unchanged and we did not observe alterations in basal or ATP-induced nociceptive firing in the meningeal afferents.ConclusionsIn summary, the lack of meningeal lymphatic system does not induce migraine-like nociceptive stateper se, but leads to a new balance between pro- and antiinflammatory factors implicated in migraine mechanisms.
Novel class of OTU deubiquitinases regulate substrate ubiquitination uponLegionellainfection
Authors: Shin Donghyuk , Bhattacharya Anshu , Cheng Yi-Lin , Alonso Marta Campos , Mehdipour Ahmad Reza , van der Heden van Noort Gerbrand J. , Ovaa Huib , Hummer Gerhard , Dikic Ivan 
Published: April 2020

AbstractLegionella pneumophilais a gram-negative pathogenic bacterium that causes Legionaries’ disease. TheLegionellagenome codes more than 300 effector proteins able to modulate host-pathogen interactions during infection. Among them are also enzymes altering the host-ubiquitination system including bacterial ligases and deubiquitinases. In this study, based on homology-detection screening on 305Legionellaeffector proteins, we identified twoLegionellaOTU-like deubiquitinases (LOT; LotB (Lpg1621/Ceg23) and LotC (Lpg2529), LotA (Lpg2248/Lem21) is already known). A crystal structure of LotC catalytic core (LotC14-310) was determined at 2.4 Å and compared with other OTU deubiquitinases, including LotB. Unlike the classical OTU-family, the structures ofLegionellaOTU-family (LotB and LotC) shows an extended helical lobe between the Cys-loop and the variable loop, which define a novel class of OTU-deubiquitinase. Despite structural differences in their helical lobes, both LotB and LotC interact with ubiquitin. LotB has an additional ubiquitin binding site (S1’) enabling specific cleavage of Lys63-linked poly-ubiquitin chains. By contrast, LotC only contains the S1 site and cleaves different species of ubiquitin chains. MS analysis of catalytically inactive LotB and LotC identified different categories of host-substrates for these two related DUBs. Together, our results provide new structural insights of bacterial OTU deubiquitinases and indicate distinct roles of bacterial deubiquitinases in host-pathogen interactions.
Binding Behavior of Microbial Functional Amyloids on Solid Surfaces
Authors: Yuca Esra , Kehribar Ebru Şahin , Şafak Şeker Urartu Özgür 
Published: April 2020

ABSTRACTSelf-assembling protein subunits hold great potential as biomaterials with improved functions. Among the self-assembled protein structures functional amyloids are promising unique properties such as resistance to harsh physical and chemical conditions their mechanical strength, and ease of functionalization. Curli proteins, which are functional amyloids of bacterial biofilms can be programmed as intelligent biomaterials. In order to obtain controllable curli based biomaterials for biomedical applications, and to understand role of each of the curli forming monomeric proteins (namely CsgA and CsgB from Escherichia coli) we characterized their binding kinetics to gold, hydroxyapatite, and silica surfaces. We demonstrated that CsgA, CsgB, and their equimolar mixture have different binding strengths for different surfaces. On hydroxyapatite and silica surfaces, CsgB is the crucial element that determines the final adhesiveness of the CsgA-CsgB mixture. On the gold surface, on the other hand, CsgA controls the behavior of the mixture. Those findings uncover the binding behavior of curli proteins CsgA and CsgB on different biomedically valuable surfaces to obtain a more precise control on their adhesion to a targeted surface.
Joint epitope selection and spacer design for string-of-beads vaccines
Authors: Dorigatti Emilio , Schubert Benjamin 
Published: April 2020

AbstractMotivationConceptually, epitope-based vaccine design poses two distinct problems: (1) selecting the best epitopes eliciting the strongest possible immune response, and (2) arranging and linking the selected epitopes through short spacer sequences to string-of-beads vaccines so as to increase the recovery likelihood of each epitope during antigen processing. Current state-of-the-art approaches solve this design problem sequentially. Consequently, such approaches are unable to capture the inter-dependencies between the two design steps, usually emphasizing theoretical immunogenicity over correct vaccine processing and resulting in vaccines with less effective immunogencity.ResultsIn this work, we present a computational approach based on linear programming that solves both design steps simultaneously, allowing to weigh the selection of a set of epitopes that have great immunogenic potential against their assembly into a string-of-beads construct that provides a high chance of recovery. We conducted Monte-Carlo cleavage simulations to show that, indeed, a fixed set of epitopes often cannot be assembled adequately, whereas selecting epitopes to accommodate proper cleavage requirements substantially improves their recovery probability and thus the effective immunogenicity, pathogen, and population coverage of the resulting vaccines by at least two fold.AvailabilityThe software and the data analyzed are available athttps://github.com/SchubertLab/JessEV
Who are you? Inconsistent identity reporting in online labor markets
Authors: Kuselias Stephen 
Published: April 2020

Purpose
Online labor pools continue to grow in popularity, serving as an inexpensive, readily available source of research data. Despite early skepticism, accounting research has generally found evidence that supports the use of these labor pools. However, one important distinction unique to online labor markets is the pre-screening process that qualifies participants for future studies. As the identity of online participants are generally unknown, researchers rely on participants’ self-reported identities to establish a pool of qualified respondents. This paper aims to provide evidence of the reliability of online participants’ answers to pre-screening questions.

Design/methodology/approach
Following the current literature’s recommendations on pre-screening candidates, I employ a multi-stage design using two similar surveys that are taken by each participant. I compare participants' answers on the first survey and the second survey to provide evidence on the consistency of their responses.

Findings
My results indicate that online participants are responding with substantial inconsistency to survey questions related to their social identity at a rate that may not be tolerable for many researchers. This has implications for researchers who use these online labor markets to represent a particular population of interest.

Originality/value
This study is the first to provide evidence on the consistency of online labor market participant responses. Additionally, it is the first to test the efficacy of current recommended guidelines for identifying populations of interest in the literature.
Innovation management in market-oriented SMEs: learning and internal arrangements for innovation
Authors: Didonet Simone , Diaz-Villavicencio Guillermo 
Published: April 2020

PurposeThis study aims to investigate the role of market orientation (MO) in improving learning for innovation in small and medium-sized enterprises (SMEs) through the facilitation of the organisational structure and links to innovation.Design/methodology/approachThe data for this research were collected through a survey that was applied to a sample of 169 SMEs in Ecuador. Existing scales were used to measure all the studied variables, i.e. MO, organisational structure for innovation, linkages and learning for innovation. The last three variables correspond to the characteristics of the innovation management process. Partial least square path modelling was used to analyse the data using the SmartPLS 2.0 software.FindingsThe results showed that MO enhances the learning for innovation in firms both directly and indirectly through improving organisational structure and linkages towards innovation. The research finding also showed that organisational structure for innovation is more important to enhance learning than linkages. Specifically, the mediation between MO and learning through linkages is smaller than the mediation through the organisational structure.Practical implicationsThe study informs executives of the relevance of developing MO as a way of improving learning for innovation, which in turn, is favoured by an organisational structure that supports creativity and technological changes and by the internal and external linkages for innovation in market-oriented firms.Originality/valueThe findings of this study provide new insights regarding how MO can work together in an innovative context and highlight the importance of MO as an enabler of innovation characteristics in SMEs. This study also contributes to the existent innovation literature by shedding light on strategic questions regarding the development of innovation process in market-oriented SMEs. Specifically, it provides some evidence regarding the nature of innovation process in SMEs, which can orient future studies focused on the understanding of how successful innovation occurs.
Middle childhood vulnerability to drugs and alcohol
Authors: Ewin Rob , Reed Abi , Powell Lewis 
Published: April 2020

Purpose
There are identified problems facing law enforcement in the correct approach to childhood drug and alcohol use at street level which can cause aggression, developmental, psychological problems and family conflict (Maher and Dixon, 1999). Childhood exposure to drugs and alcohol can encourage criminal activity, anti-social conduct and increased child-to-parent conflict (Brook et al., 1992; Reinherz et al., 2000; Coogan, 2011; McElhone, 2017).

Design/methodology/approach
The purpose of this study is to explore middle-childhood (11-15 years) experiences of drugs and alcohol through a survey to determine the earliest opportunity for the involvement of services based on the experiences of children.

Findings
The key findings are alcohol consumption in middle childhood is supported by parental alcohol provision; those in middle childhood are most likely to consume alcohol at home and drugs at street level (any place away from home including school, young clubs, open public space and parks); children in middle childhood use mainly cannabis to experience euphoria, minimize childhood problems and to fulfill acquisitive desire; and late childhood shows movement away from street-level drug use to drug use in private spaces with friends and increased levels of experiential or social drinking, within spaces shared by larger social groups.

Practical implications
The authors propose that a health-orientated early help model in middle childhood should be adopted, with support such as community- and school-based child and parental drug education; wider information sharing between schools, policing and health authorities at an early stage to support a contextual safeguarding approach; and recognition and recording practices around middle childhood which is an acute phase for children to become involved in drug and alcohol consumption.

Originality/value
Children’s drug use in middle childhood is often not recorded, and the problem can be associated with simple ill-parenting approaches. The authors believe that little was known about the spaces and occurrence of drug and alcohol use in middle childhood.
Older people's perspectives on living in integrated housing and care settings: the case of extra care housing
Authors: Cameron Ailsa , Johnson Eleanor K , Evans Simon 
Published: April 2020

PurposeThis paper explores residents' perceptions and experiences of extra care housing as an integrated model of housing with care.Design/methodology/approachData were collected in a longitudinal qualitative study based on four extra care housing schemes. Data from interviews with residents, care workers, managers and local commissioners were analysed thematically.FindingsThe integration of housing with care enabled many older people to manage their care proactively. However, the increasing number of residents with complex health and care needs, including chronic illness, led some residents to question the ability of the model to support residents to live independently.Research limitations/implicationsThe study struggled to recruit sufficient residents from the specialist dementia setting who were able to communicate their consent to take part in the research. In addition, the quality of qualitative data collected in interviews with participants at this setting reduced over successive rounds of interviews.Practical implicationsThe study suggests the need to ensure that residents are fully informed about levels of care and support is available when considering a move into extra care housing.Originality/valueThis paper provides a timely opportunity to consider extra care housing as an example of an integrated housing service, particularly in light of the current challenges facing the sector.
Entrepreneurial university ecosystems and graduates' career patterns: do entrepreneurship education programmes and university business incubators matter?
Authors: Guerrero Maribel , Urbano David , Gajón Eduardo 
Published: April 2020

PurposeThis paper provides insights about how graduates' career patterns (i.e. academic entrepreneur, self-employed or paid employed) are influenced by entrepreneurial university ecosystems (i.e. incubators and entrepreneurship education programs).Design/methodology/approachBy adopting Douglas and Shepherd's utility-maximising function, the influence of one entrepreneurial university ecosystem on graduates' career choices was tested using a sample of 11,512 graduates from the Monterrey Institute of Technology and Higher Education (ITESM) in Mexico.FindingsOur results show the critical role of entrepreneurial universities ecosystems in facilitating employability options as academic entrepreneurship for ITESM's graduates. The study shows some insights about how graduates' risk aversion and work effort are positively influenced by the university business incubator and entrepreneurship education programs, respectively.Practical implicationsDiverse implications for stakeholders have emerged from our results. These implications are associated with potential benefits of implementing programmes oriented to engage academic entrepreneurship within Latin American universities.Originality/valueEntrepreneurial universities provide a range of employability alternatives for their students, such as to be self-employed, academic entrepreneurs or paid employees. In this scenario, entrepreneurial universities have configured entrepreneurial ecosystems (educational programmes, business incubators and other infrastructures) to support potential entrepreneurs (students, academics, staff and alumni). Despite the relevance of the environmental conditions on individuals' occupational choices, few studies have explored the role of the entrepreneurial university ecosystems on graduates' employability. In this vein, our study contributes to some academic discussions: (1) the role of context on career choice models (Ilougaet al., 2014; Sieger and Monsen, 2015), (2) the role of incubators and entrepreneurship education on fostering academic entrepreneurship on the graduates' community (Nabiet al., 2017; Goodet al., 2019; Guerrero and Urbano, 2019a) and (3) the effectiveness of the entrepreneurial university ecosystems on graduates' employability (Herreraet al., 2018; Wrightet al., 2017).
Women's progress in the Arab world: classroom–boardroom pipeline
Authors: Kemp Linzi J 
Published: April 2020

PurposeThe purpose of this conceptual paper was to investigate the contribution of the “Pipeline”, as a metaphor for building theory about Women-on-Boards (WoB) in the Arab world.Design/methodology/approachNarratives about women's progress in Arab countries were collected from a range of sources and content was analysed to identify emergent themes about pipeline.FindingsThemes were identified of the pipeline metaphor that explained phenomena and generated solutions to employ, retain and advance women to board directorships; from higher education (“bulging”/“bursting” pipeline) through employment (“leaking” pipeline) to boardroom (“blocked” pipeline).Research limitations/implicationsGeneralisation of these study results is limited by geographical context of this research. An implication is for further international studies on metaphor identification for women's progress.Practical implicationsRelevant metaphor-in-use required to generate company policy and praxis towards WoB in the Arab world.Originality/valueThe first academic study to investigate the value of metaphor for effect on women's progress in Arab countries. Novel metaphor identification is proposed to think and see women's experiences in cultural context.
Negative publicity and potential applicants' intention to apply amid a discrimination scandal: a moderated mediation model
Authors: Ouyang Zhe , Zhang Yuanyuan , Hu Xi 
Published: April 2020

PurposeThe exposure of recruitment discrimination often leads to negative publicity, which can substantially affect organizational attractiveness and the behavioral intentions of stakeholders. Therefore, this study aims to examine the relationship between negative publicity and intention to pursue a job, with organizational attractiveness as a mediator and perceived corporate social responsibility (CSR) and corporate product quality (CPQ) as two moderators.Design/methodology/approachA survey completed by 382 college students in three universities in Nanjing, China, was used to empirically test the research hypotheses. The respondents were asked their intention to apply for a job at Meituan after the exposure of its recruitment discrimination scandal in 2017.FindingsPerceived negative publicity negatively influences intention to pursue employment via organizational attractiveness. In addition, moderated path analysis indicated that perceived CSR strengthens and perceived CPQ weakens the direct effect of perceived negative publicity on organizational attractiveness and its indirect effect on intention to pursue employment.Research limitations/implicationsFuture research should seek to replicate these findings in other contexts and populations, including people who are not new to the job market and to control additional firm-level and contextual variables.Originality/valueThis research confirms a moderated mediation model positioning organizational attractiveness as a mediator of negative publicity's effects on intention to pursue employment and organizational image as a moderator of such effects. This study also contributes to the debate concerning the effect of corporate image by demonstrating opposing effects of its different dimensions amid a discrimination scandal.
Sleepy but creative? How affective commitment, knowledge sharing and organizational forgiveness mitigate the dysfunctional effect of insomnia on creative behaviors
Authors: De Clercq Dirk , Pereira Renato 
Published: April 2020

PurposeThis study investigates how employees' experience of suffering from insomnia might reduce the likelihood that they perform creative activities, as well as how this negative relationship might be buffered by employees' access to resources at three levels: an individual resource (affective commitment), a relational resource (knowledge sharing with peers) and an organizational resource (climate of organizational forgiveness).Design/methodology/approachQuantitative data came from a survey of employees in the banking sector.FindingsInsomnia reduces creativity, but this effect is weaker when employees feel a strong emotional bond to their organization, openly share knowledge with colleagues and believe that their organization forgives errors.Research limitations/implicationsThe limitations of this research include its relatively narrow scope by focusing on one personal stressor only, its cross-sectional design, its reliance on subjective measures of insomnia and creativity and its single-industry, single-country design.Practical implicationsThe findings indicate different, specific ways in which human resource managers can overcome the challenges associated with sleep-deprived employees who avoid productive work behaviors, including creativity.Originality/valueThis study adds to extant scholarship by specifying how employees' persistent sleep deprivation might steer them away from undertaking creative behaviors, with a particular focus on how several pertinent resources buffer this process.
Fourth-graders' reading, thinking, and writing about historical sources
Authors: Bickford John H. , Clabough Jeremiah , Taylor Tim N. 
Published: April 2020

PurposeElementary classroom teachers can infuse social studies into the curriculum by integrating history, civics and English/language arts. Elementary teachers can bundle close reading, critical thinking and text-based writing within historical inquiries using accessible primary sources with engaging secondary sources.Design/methodology/approachThis article reports the successes and struggles of one fourth-grade teacher's theory-into-practice interdisciplinary unit. The month-long, history-based inquiry integrated close readings of primary and secondary sources to scaffold and refine students' text-based writing about the oft-ignored interconnections between two Civil Rights icons who never met.FindingsFindings included the import of historical inquiries within the elementary grades, students' abilities to scrutinize and extract meaning from dozens of sources and the value of revision for text-based writing, particularly its impact on the clarity, criticality and complexity of students' writing.Originality/valueThe inquiry's length, use of repeated readings, bulk of curricular resources and integration of revision are each comparably unique within the elementary social studies research literature.