bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of lipid coverage and high spatial resolution MALDI-imaging capabilities of oversampling combined with laser post-ionisation
Authors: Bowman Andrew P. , Bogie Jeroen F. J. , Hendriks Jerome J. A. , Haidar Mansour , Belov Mikhail , Heeren Ron M. A. , Ellis Shane R. 
Published: December 2019

AbstractMatrix-assisted laser desorption/ionisation-mass spectrometry imaging (MALDI-MSI) is a powerful technique for visualising the spatial locations of lipids in biological tissues. However, a major challenge in interpreting the biological significance of local lipid compositions and distributions detected using MALDI-MSI is the difficulty in associating spectra with cellular lipid metabolism within the tissue. By-and-large this is due to the typically limited spatial resolution of MALDI-MSI (30–100 μm) meaning individual spectra represent the average spectrum acquired from multiple adjacent cells, each potentially possessing a unique lipid composition and biological function. The use of oversampling is one promising approach to decrease the sampling area and improve the spatial resolution in MALDI-MSI, but it can suffer from a dramatically decreased sensitivity. In this work we overcome these challenges through the coupling of oversampling MALDI-MSI with laser post-ionisation (MALDI-2). We demonstrate the ability to acquire rich lipid spectra from pixels as small as 6 μm, equivalent to or smaller than the size of typical mammalian cells. Coupled with an approach for automated lipid identification, it is shown that MALDI-2 combined with oversampling at 6 μm pixel size can detect up to three times more lipids and many more lipid classes than even conventional MALDI at 20 μm resolution in the positive-ion mode. Applying this to mouse kidney and human brain tissue containing active multiple sclerosis lesions, where 74 and 147 unique lipids are identified, respectively, the localisation of lipid signals to individual tubuli within the kidney and lipid droplets with lesion-specific macrophages is demonstrated.
Boat disturbance effects on moulting common eiders Somateria mollissima
Authors: Dehnhard Nina , Skei Jørgen , Christensen-Dalsgaard Signe , May Roel , Halley Duncan , Ringsby Thor Harald , Lorentsen Svein-Håkon 
Published: December 2019

AbstractShip and boat traffic are increasing sources of disturbance to marine wildlife. During moult, sea ducks are flightless and rely on productive and shallow feeding areas. However, this period coincides with the peak of the recreational boating season. This is the first study to investigate the escape behaviour of moulting common eiders (Somateria mollissima) to the approach of small boats. We quantified flight initiation distances (flock-to-boat distance at which an energy-demanding escape occurred), displacement distances (distance between the pre- and post-disturbance position of the flock) and the time it took flocks to return to pre-disturbance (foraging- or resting-) behaviour. Moulting common eiders showed average flight initiation distances of 177 m and displacement distances of 771 m. Displacement distances decreased with flock size, under higher wind speeds and when previous foraging habitat was shallower. Time-to-return to pre-disturbance behaviour decreased with flock size but increased with wind speed and accessibility of foraging habitat at the previous location. Most (75%) of flocks returned to pre-disturbance behaviour within 10 min after the disturbance, while three flocks kept disturbed even 45 min after the approach. Finally, flocks encountered less accessible (deeper) habitats after disturbance than before. Our results suggest that approaching boats imply considerable disturbance effects for moulting common eiders through increased locomotion costs, displacement from accessible foraging habitat and/or time lost for foraging or resting. We provide valuable information for policy makers and marine spatial planning and highlight the need for awareness among recreational boat drivers on their impact on wildlife.
Percutaneous endoscopic debridement with percutaneous pedicle screw fixation for lumbar pyogenic spondylodiscitis: a preliminary study
Authors: Duan Kaidi , Qin Yi , Ye Jichao , Zhang Wei , Hu Xumin , Zhou Jinlang , Gao Liangbin , Tang Yong 
Published: December 2019

Abstract
Purpose
To access the feasibility and efficacy of percutaneous endoscopic debridement (PED) combined with percutaneous pedicle screw fixation (PPSF) in the treatment of lumbar pyogenic spondylodiscitis.

Methods
Forty-five patients diagnosed as pyogenic spondylodiscitis underwent PPSF followed by PED. A drainage catheter was left in place for negative pressure drainage. Adequate systematic antibiotics were administered empirically or based on bacterial culture results. Clinical outcomes were assessed by physical examination, regular serologic testing, visual analog scale (VAS), Oswestry Disability Index (ODI), and imaging studies.

Results
The mean operative time was 110.1 ± 21.2 minutes (range 80–165 minutes), with intra-operative blood loss 47.8 ± 21.0 ml (range 20–120 ml). All patients reported relief of back pain, able to sit up, and partially ambulate the next day. Causative pathogens were identified in 32 of 45 biopsy specimens, staphylococcal bacteria being the most prevalent strain. However, there were 13 patients with post-operative complications. During 6–12 months’ follow-up, inflammatory markers showed infection controlled. VAS and ODI values were significantly improved.

Discussion
Satisfactory clinical and functional outcomes were achieved in our patients post-operatively. It is recommended that PED plus PPSF can be another alternative for spondylodiscitis.

Conclusion
PED supplementing PPSF offers a valid option in treating spondylodiscitis, as it is minimally invasive, shortens hospital stay, and avoids prolonged bed rest with an optimistic outcome.
Coping styles in capital breeders modulate behavioural trade-offs in time allocation: assessing fine-scale activity budgets in lactating grey seals (Halichoerus grypus) using accelerometry and heart rate variability
Authors: Shuert Courtney R. , Pomeroy Patrick P. , Twiss Sean D. 
Published: December 2019

Abstract
Balancing time allocation among competing behaviours is an essential part of energy management for all animals. However, trade-offs in time allocation may vary according to the sex of the individual, their age, and even underlying physiology. During reproduction, higher energetic demands and constrained internal resources place greater demand on optimizing these trade-offs insofar that small adjustments in time-activity may lead to substantial effects on an individual’s limited energy budget. The most extreme case is found in animals that undergo capital breeding, where individuals fast for the duration of each reproductive episode. We investigated potential underlying drivers of time-activity and describe aspects of trade-offs in time-activity in a wild, capital breeding pinniped, the grey seal Halichoerus grypus, during the lactation period. For the first time, we were able to access full 24-h activity budgets across the core duration of lactation as well as characterize how aspects of stress-coping styles influence time allocation through the use of animal-borne accelerometers and heart rate monitors in situ. We found that there was a distinct trade-off in time activity between time spent Resting and Alert (vigilance). This trade-off varied with the pup’s development, date, and maternal stress-coping style as indicated by a measure of heart rate variability, rMSSD. In contrast, time spent Presenting/Nursing did not vary across the duration of lactation given the variables tested. We suggest that while mothers balance time spent conserving resources (Resting) against time expending energy (Alert), they are also influenced by the inherent physiological drivers of stress-coping styles.

Significance statement
How animals apportion their time among different behaviours is key to their success. These trade-offs should be finely balanced to avoid unnecessary energy expenditure. Here, we examine how grey seal mothers balance their activity patterns during the short, but energetically demanding, period of pup-rearing. Animal-borne accelerometers provided a uniquely detailed and continuous record of activity during pup-rearing for 38 mothers. We also used heart rate monitors to provide measures of each individual’s stress-coping style. We found that mothers balance time Resting against remaining Alert while time Presenting/Nursing was largely independent of all factors measured. Stress-coping styles were found to drive the balancing and variation of all behaviours. This novel indication that differences in personality-like traits may drive whole activity budgets should be considered when assessing trade-offs in time allocation across a much wider variety of species.
Effect of rifampin and itraconazole on the pharmacokinetics of zanubrutinib (a Bruton's tyrosine kinase inhibitor) in Asian and non-Asian healthy subjects
Authors: Mu Song , Tang Zhiyu , Novotny William , Tawashi Manal , Li Ta-Kai , Ou Ying , Sahasranaman Srikumar 
Published: December 2019

Abstract
Purpose
Zanubrutinib (BGB-3111) is a potent Bruton’s tyrosine kinase inhibitor with promising clinical activity in B-cell malignancies. Zanubrutinib was shown to be mainly metabolized through cytochrome P450 3A (CYP3A) in vitro. We evaluated the effect of steady-state rifampin (a strong CYP3A inducer) and steady-state itraconazole (a strong CYP3A inhibitor) on the pharmacokinetics (PK), safety, and tolerability of zanubrutinib in healthy Asian and non-Asian subjects.

Methods
In this open-label, two-part clinical study, 20 participants received a single oral dose of zanubrutinib (320 mg) and oral rifampin (600 mg) in Part A, and 18 participants received a single oral dose of zanubrutinib (20 mg) and oral itraconazole (200 mg) in Part B. Serial blood samples were collected after administration of zanubrutinib alone and zanubrutinib in combination with rifampin or itraconazole for the measurement of PK parameters.

Results
Coadministration with rifampin decreased AUC0–∞ of zanubrutinib by 13.5-fold and Cmax by 12.6-fold. Coadministration with itraconazole increased the AUC0–∞ of zanubrutinib by 3.8-fold and Cmax by 2.6-fold. The PK of zanubrutinib was consistent between Asian and non-Asian subjects, and zanubrutinib was well tolerated in this study.

Conclusions
These results confirm that zanubrutinib is primarily metabolized by CYP3A in humans. The PK of zanubrutinib was comparable between Asian and non-Asian subjects and, therefore, no dose modifications are necessary for zanubrutinib in these ethnic populations.
Impact of Arctic amplification on declining spring dust events in East Asia
Authors: Liu Jun , Wu Dongyou , Liu Guangjing , Mao Rui , Chen Siyu , Ji Mingxia , Fu Pingqing , Sun Yele , Pan Xiaole , Jin Hongchun , Zhou Yubin , Wang Xin 
Published: December 2019

AbstractDust aerosols play key roles in affecting regional and global climate through their direct, indirect, and semi-direct effects. Dust events have decreased rapidly since the 1980s in East Asia, particularly over northern China, primarily because of changes in meteorological parameters (e.g. surface wind speed and precipitation). In this study, we found that winter (December–January–February) Arctic amplification associated with weakened temperature gradients along with decreased zonal winds is primarily responsible for the large decline in following spring (March–April–May) dust event occurrences over northern China since the mid-1980s. A dust index was developed for northern China by combining the daily frequency of three types of dust event (dust storm, blowing dust, and floating dust). Using the empirical orthogonal function (EOF) analysis, the first pattern of dust events was obtained for spring dust index anomalies, which accounts for 56.2% of the variability during 1961–2014. Moreover, the enhanced Arctic amplification and stronger Northern Hemisphere annular mode (NAM) in winter can result in the anticyclonic anomalies over Siberia and Mongolia, while cyclonic anomalies over East Europe in spring. These results are significantly correlated with the weakened temperature gradients, increased precipitation and soil moisture, and decreased snow cover extent in the mid-latitude over Northern Hemisphere. Based on the future predictions obtained from the Fifth Climate Models Intercomparison Project (CMIP5), we found that the dust event occurrences may continually decrease over northern China due to the enhanced Arctic amplification in future climate.
Mechanical complications in adult spinal deformity and the effect of restoring the spinal shapes according to the Roussouly classification: a multicentric study
Authors: Sebaaly Amer , Gehrchen Martin , Silvestre Clément , Kharrat Khalil , Bari Tanvir Johanning , Kreichati Gabi , Rizkallah Maroun , Roussouly Pierre 
Published: December 2019

Abstract
Purpose
To evaluate the incidence of mechanical complications in patients with adult spine deformity (ASD) treated by restoring the normal shape according to the Roussouly classification.

Methods
This is a retrospective multicentric study with a minimum follow-up of 2 years. Patients operated on with fusion for ASD (minimum performed fusion: L2 to sacrum) were included. Patients with a history of previous spinal fusion of more than three levels were excluded. Spinal and pelvic parameters were measured on the preoperative and the immediate postoperative follow-up. All mechanical complications were recorded.

Results
A total of 290 patients met the criteria of inclusion with a minimum follow-up of 2 years. Mechanical complications occurred in 30.4% of the cohort. The most common complication was PJK with an incidence of 18% while nonunion or instrumentation failure (rod breakage, implant failure) occurred in 12.4%. 66% of the patients were restored to the normal shape according to the Roussouly classification based on their PI and had a mechanical complication rate of 22.5%, whereas the remaining 34% of patients had a complication rate of 46.8% (p < 0.001). The relative risk for developing a mechanical complication if the algorithm was not met was 3 (CI 1.5–4.3; p < 0.001)

Conclusion
In the recent literature, there are no clear guidelines for ASD correction. Restoring the sagittal spinal contour to the normal shapes of Roussouly according to the PI could serve as a guideline for ASD treatment. Ignoring this algorithm has a threefold risk of increased mechanical complications. We recommend this algorithm for treatment of ASD.

Level of evidence
IV cross-sectional observational study.

Graphic abstract
These slides can be retrieved under Electronic Supplementary Material.
Evaluating the user experience of acoustic data transmission
Authors: Mehrabi Adib , Mazzoni Antonella , Jones Daniel , Steed Anthony 
Published: December 2019

AbstractUsers of smart devices frequently need to exchange data with people nearby to them. Yet despite the availability of various communication methods, data exchange between co-located devices is often complicated by technical and user experience barriers. A potential solution to these issues is the emerging technology of device-to-device acoustic data transmission. In this work, we investigate the medium-specific properties of sound as a data exchange mechanism, and question how these contribute to the user experience of sharing data. We present a user study comparing three wireless communication technologies (acoustic data transmission, QR codes and Bluetooth), when used for a common and familiar scenario: peer-to-peer sharing of contact information. Overall, the results show that acoustic data transmission provides a rapid means of transferring data (mean transaction time of 2.4 s), in contrast to Bluetooth (8.3 s) and QR (6.3 s), whilst requiring minimal physical effort and user coordination. All QR code transactions were successful on the first attempt; however, some acoustic (5.6%) and Bluetooth (16.7%) transactions required multiple attempts to successfully share a contact. Participants also provided feedback on their user experience via surveys and semi-structured interviews. Perceived transaction time, physical effort, and connectivity issues. Specifically, users expressed frustration with Bluetooth due to device selection issues, and with QR for the physical coordination required to scan codes. The findings indicate that acoustic data transmission has unique advantages in facilitating information sharing and interaction between co-located users.
Transparent experiments: releasing data from mechanical tests on three dimensional hydrogel sphere packings
Authors: Barés Jonathan , Brodu Nicolas , Zheng Hu , Dijksman Joshua A. 
Published: December 2019

AbstractWe describe here experiments on the mechanics of hydrogel particle packings from the Behringer lab, performed between 2012 and 2015. These experiments quantify the evolution of all contact forces inside soft particle packings exposed to compression, shear, and the intrusion of a large intruder. The experimental set-ups and processes are presented and the data are concomitantly published in a repository (Barés et al. in Dryad, Dataset 10.5061/dryad.6djh9w0x8, 2019).
The association between uric acid levels and renal function of CKD patients with hyperlipidemia: a sub-analysis of the ASUCA trial
Authors: Kuwabara Yoshihiro , Yasuno Shinji , Kasahara Masato , Ueshima Kenji , Nakao Kazuwa 
Published: December 2019

Abstract
Background
The influence of uric acid (UA) on renal function and the significance of UA-lowering therapy are unclear. The purpose of the sub-analysis of the Assessment of Clinical Usefulness in chronic kidney disease patients with Atorvastatin (ASUCA) trial was to evaluate the influence of serum UA levels on renal function in Japanese chronic kidney disease patients with hyperlipidemia.

Methods
Of 344 participants in the ASUCA trial, 279 participants whose UA levels at both baseline and 24 months were available were included. Based on UA level at baseline or mean UA level during the trial period, they were divided into four groups: < 5.0, 5.0–6.0, 6.0–7.0, or ≥ 7.0 mg/dL, irrespective of allocation. Changes in the estimated glomerular filtration rate (eGFR) after 24 months were compared among the groups in relation to baseline or mean UA levels.

Results
For baseline UA levels (< 5.0, 5.0–6.0, 6.0–7.0, or ≥ 7.0 mg/dL), the change in eGFR after 24 months was − 1.32 ± 10.3, − 1.74 ± 8.94, − 2.53 ± 7.34, and − 3.51 ± 9.10 mL/min/1.73 m2, respectively. A negative correlation between changes in eGFR after 24 months and baseline UA level was observed with adjustment for confounding factors. The relationship between changes in eGFR and mean UA levels during trial period showed a similar trend.

Conclusion
In CKD patients with dyslipidemia, hyperuricemia was an independent risk factor for CKD progression. An ongoing clinical trial (TARGET-UA, UMIN-ID 000,026,741) may reveal the significance of strict UA-lowering therapy in CKD patients.
Prison Leave in Finland: Legal and Empirical Fundamentals of an Established Practice
Authors: Keinänen A. , Kilpeläinen M. , Pajuoja J. , Tyni S. 
Published: December 2019

AbstractPrison leave was introduced into the Finnish legal system in 1971, with the aim to reduce negative effects of institutionalization and disadvantages caused by the length of the prison sentence. After the total reform of prison legislation in 2006, the role of the prison leave has become even more central; the number of prisoners has decreased, but the amount of prison leave has increased. Historically, the length of the sentence has been the most common ground for prison leave. A prisoner can be granted a prison leave when two thirds of the prison term has been served, for example, after 2 years if the length of the total sentence served in prison is 3 years. However, during the past 10 years, prison leave based on an important reason has grown into the most common type of prison leave. This indicates a structural change from the rigid legal rules to a more flexible practice. In 2018, there were about 17,000 prison leave applications, and over 13,000 of those were granted, i.e. 79%. The conditions were breached 466 times, which is 3.5% of all prison leaves. The most common breaches of prison leave conditions were returning from a prison leave after the set time limit or under the influence of alcohol or drugs. The majority of prison leave applications are decided in the prisons. On the other hand, for example, the decision on the prison leave of a life-sentenced prisoner is made by the Criminal Sanctions Agency. There have been significant differences in the probability of granting prison leave, which are emphasized especially in the practices of closed prisons. Among those prisoners who serve longer than 1 year in prison, the application rate of prison leave rises over 90%. For the sentences under 3 months, it is less than 20%.
Underpricing of initial public offerings in experimental asset markets
Authors: Füllbrunn Sascha , Neugebauer Tibor , Nicklisch Andreas 
Published: December 2019

AbstractThe underpricing of initial public offerings (IPO) is a well-documented fact of empirical equity market research. Theories explain this underpricing with market imperfections. We study three empirically relevant IPO mechanisms under almost perfect market conditions in the laboratory: a stylized book building approach, a closed book auction, and an open book auction. We report underpricing in each of these IPO mechanisms. Uncertainty about the aftermarket behavior may partly explain IPO excess returns but underpricing persists even in the repeated setting where uncertainty is negligible and despite the equilibrium adjustment dynamics, that we observe in the data. The data reveal a market-wide impact of investors’ reluctance to sell in the aftermarket at a price below the offering price. We conclude that a behavioural bias similar to the disposition effect fosters IPO underpricing in our setting.
Challenge-Driven Innovation Policy: Towards a New Policy Toolkit
Authors: Mazzucato Mariana , Kattel Rainer , Ryan-Collins Josh 
Published: December 2019

AbstractPolicy makers are increasingly embracing the idea of using industrial and innovation policy to tackle the ‘grand challenges’ facing modern societies. This article argues that through well-defined goals, or more specifically ‘missions’, that are focused on solving important societal challenges, policymakers have the opportunity to determine thedirectionof growth by making strategic investments across many different sectors and nurturing new industrial landscapes, which the private sector can develop further, and as a result induce cross-sectoral learning and increase macroeconomic stability. This ‘mission-oriented’ approach to industrial policy is not about ‘top down’ planning by an overbearing state; it is about providing a direction for growth and increasing business expectations about future growth areas and catalysing activity that otherwise would not happen. It is not about de-risking and levelling the playing field, nor about supporting more competitive sectors over less since the market does not always ‘know best’ but tilting the playing field in the direction of the desired societal goals, such as the sustainable development goals. To achieve this requires a different policy framework, what we call the ‘ROAR’ framework, which involves strategic thinking about the desired direction of travel (Routes), the structure and capacity of public sectorOrganisations, the way in which policy isAssessedand the incentive structure for both private and public sectors (Risks and Rewards). The article argues that if we want to take grand challenges such as the SDGs seriously as policy goals, market shaping should become the over-arching approach followed in various policy fields.