bluuebunny/crossref_metadata_2025_split huggingface dataset:
Active-site Molecular docking of Nigellidine to nucleocapsid/Nsp2/Nsp3/MPro of COVID-19 and to human IL1R and TNFR1/2 may stop viral-growth/cytokine-flood, and the drug source Nigella sativa (black cumin) seeds show potent antioxidant role in experimental rats.
Authors: Maiti Smarajit , Banerjee Amrita , Nazmeen Aarifa , Kanwar Mehak , Das Shilpa 
Published: May 2020

Abstract
The recent outbreak of SARS CoV-2 has changed the global scenario of human lives and economy. In this pandemic-outbreak the ratio of infected person is much higher than the death encountered. Most of the dead patients were observed with dysfunction/failure of cardiac and renal systems. Beside this a ‘cytokine storm’ namely TNF-α/IL1 receptors i.e. TNFR1/TNFR2/IL1R over-functioning was reported in the infected-persons. Here, nigellidine, an indazole-alkaloid and key-component of Nigella Sativa L. (NS); black-cumin-seed, has been analyzed for COVID-19 different protein and TNFα receptors TNFR1/TNFR2 and IL1R inhibition through molecular-docking study and biochemical-study of cumin-seed extract exposure to experimental-rat. The NMR, X-ray-crystallographic or Electron-microscopic structures of COVID-19 Main-protease(6LU7), Spike-glycoprotein(6vsb), NSP2(QHD43415_2), N-terminus-protenase (QHD43415_3), Nucleocapsid(QHD43423) and Human IL1R (1itb), TNFR1 (1ncf), TNFR2 (3alq) from PDB were retrieved/analyzed for receptor-ligand interaction in normal condition. Then those structures were docked with nigellidine using Autodock-software and Patchdock-server. Where nigellidine showed highest binding-energy of -7.61 (kcal/mol) and ligand-efficiency value of (-0.35) forming bonds with amino acids THR943/LYS945/MET1556/ALA1557/PRO1558/ILE1559. Highest ACE-value of -356.72 was also observed for nigellidine N-terminal-protease interaction. Nigellidine also showed strong interaction with NSP2 (-6.28) and Mpro/3CLpro_Q (-6.38s). Nigellidine showed affinity to TNFR1 (-6.81), IL1R (-6.23) and TNFR2 (-5.16). In rat experiment 2-groups (vehicle and NS treated) of female Wistar-rats were taken for experiments. The NS treated tissue showed marked decline in ALP/SGPT/ SGOT/MDA level then the basal-levels. From the Western-blot or activity analysis it was observed that Nigellidine, the sulfuryl-group containing drug showed no impact on Phenol-catalyzing ASTIV or Steroid-catalyzing EST expressions/activities and thus have no influence in sulfation-mediated adverse metabolic-processes. Current-results concluded that Nigellidine has hepato/reno-protective; immunomodulatory/anti-inflammatory and antioxidant activities as well as it inhibits important proteins of COVID-19. With steps to further validation/checking nigellidine can be used in COVID-19 infection.
A Deep Learning Framework for Coronavirus Disease (COVID-19) Detection in X-Ray Images
Authors: Ozcan Tayyip 
Published: May 2020

Abstract
Coronavirus, a large family of viruses, causes illness in both humans and animals. The novel coronavirus (COVID-19) came up in Wuhan in December 2019. This deadly COVID-19 pandemic has become very fast-spreading and currently present in several countries worldwide. The timely detection of patients who have COVID-19 is vitally important. To this end, scientists are working on different detection methods.In this paper, a grid search (GS) and pre-trained model aided convolutional neural network (CNN) model is proposed to detect COVID-19 in X-Ray images. In the proposed method, the GS method is employed to optimize the hyperparameters of CNN, which directly affects classification performance. Three pre-trained CNN models (GoogleNet, ResNet18 and ResNet50), which can be used for classification, feature extraction and transfer learning purposes were used for transfer learning in this study. The proposed method was trained using the training and validation subdatasets of the collected dataset and detail evaluations are presented according to different performance metrics. According to the experimental studies, the best results were obtained with the GS and ResNet50 aided model.
Multi-omics-data-assisted genomic feature preselection improves the accuracy of genomic prediction
Authors: Ye Shaopan , Li Jiaqi , Zhang Zhe 
Published: May 2020

Abstract
Background: Presently, multi-omics data (e.g., genomics, transcriptomics, proteomics, and metabolomics) are available for genomic prediction. Omics data not only offer new data layers for genomic prediction but also provide a bridge between organismal phenotypes and genome variation that cannot be readily captured at the genome sequence level. Therefore, using multi-omics data to select feature markers is a feasible strategy to improve the accuracy of genomic prediction. In this study, simultaneously using whole-genome sequencing (WGS) and gene expression level data, four strategies for single-nucleotide polymorphism (SNP) preselection were investigated for genomic predictions in the Drosophila Genetic Reference Panel.Results: Using genomic best linear unbiased prediction (GBLUP) with complete WGS data, the prediction accuracy values were 0.208±0.020 (0.181±0.022) for the startle response and 0.272±0.017 (0.307±0.015) for starvation resistance in the female (male) lines. Compared with GBLUP using complete WGS data, both GBLUP and the genomic feature BLUP (GFBLUP) did not improve the prediction accuracy using SNPs preselected from the complete WGS data based on the results of genome-wide association studies (GWASs) or transcriptome-wide association studies (TWASs). Furthermore, by using SNPs preselected from the WGS data based on the results of the expression quantitative trait locus (eQTL) mapping of all genes, only the startle response had greater accuracy than GBLUP with the complete WGS data. The best accuracy values in the female and male lines were 0.243±0.020 and 0.220±0.022, respectively. Importantly, by using SNPs preselected based on the results of the eQTL mapping of significant genes from TWAS, both GBLUP and GFBLUP resulted in a greater accuracy and smaller bias of genomic prediction. For the startle response, the best accuracy values were 0.258±0.019 (0.237±0.019) for GBLUP and 0.265±0.018 (0.245±0.020) for GFBLUP in the female (male) lines. For starvation resistance, the best accuracy values were 0.437±0.015 (0.427±0.015) for GBLUP and 0.419±0.016 (0.390±0.014) for GFBLUP in female (male) lines. Compared to the GBLUP with complete WGS data, the best accuracy values represented increases of 60.66% and 39.09% for the startle response and 27.40% and 35.36% for starvation resistance in the female and male lines, respectively. Conclusions: Overall, multi-omics data can assist genomic feature preselection and improve the performance of genomic prediction. The new knowledge gained from this study will enrich the use of multi-omics in genomic prediction.
A BUSCA PELA LEGITMIDADE DA RELAÇÃO POLIAMOROSA COM BASE EM PRINCÍPIOS CONSTITUCIONAIS E A DISCUSSÃO SOBRE A VALIDADE DAS ESCRITURAS PÚBLICAS QUE AS DECLARAM
Authors: SILVA Bruna Terra Teodoro da , TOFFANO Marcelo 
Published: May 2020

Com a promulgação da Carta Magna, houve a constitucionalização do Direito Privado. A partir desse marco, as normas do Direito de Família passaram a ser interpretadas a luz dos princípios constitucionais, fomentando o alargamento do conceito de família, que passou a ser associada como instrumento de realização pessoal; Tanto é citou exemplificativamente novos arranjos familiares, quais sejam, a família monoparentel e a união estável.Com o reconhecimento da família homoafetiva pelo STF na ADPF132 e ADI 4277, constatou-se que o artigo 226 da C.F. tratava-se realmente de norma inclusiva, isto é, o Estado está incumbido de proteger qualquer entidade familiar que tenha como base o afeto. Por conseguinte, a família poliamorosa é legal e digna de tutela jurisdicional.
A REGULAÇÃO ECONÔMICA DA MÍDIA: PARLAMENTARES PROPRIETÁRIOS DE EMISSORAS DE RÁDIO E TELEVISÃO, E O ARRENDAMENTO DA GRADE DE PROGRAMAÇÃO ÀS IGREJAS
Authors: ALMEIDA Maria Laura Felicio de , GOMES Fábio Cantizani 
Published: May 2020

O principal objetivo desta pesquisa de iniciação científica é elucidar questões controvertidas em torno da regulação da mídia no Brasil. Ademais, o trabalho analisa os diversos problemas no setor da comunicação, com ênfase na prática ilegal de aluguel da grade de programação das emissoras de rádio e televisão às igrejas, para a veiculação de conteúdos religiosos/proselitistas, e na presença de parlamentares detentores de mandato eletivo donos ou sócios de emissoras concessionárias do serviço de radiodifusão. Através da pesquisa bibliográfica e, subsidiariamente, do levantamento de dados, percebeu-se que as leis responsáveis por disciplinar a comunicação social não impedem a formação de oligopólios e propriedade cruzada nem reduzem a influência de grupos políticos e religiosos dentro da mídia. Palavras-chave: Comunicação Social; Regulação da Mídia; Regime Democrático.
Compressive and Flexural Strengths of Concrete Containing Ground Palm Kernel Shells as Partial Replacement of Cement
Authors: Armah Esau Abekah , Koffi Hubert Azoda , Amuzu Josef K Ametefe 
Published: May 2020

This study explore the possibility of using waste ground palm kernel (GPK) shells as partial replacement of cement in concrete using mechanical destructive method has been studied. The palm kernel shells were in two forms: the GPK ordinary shells and shells subjected to incomplete combustion (i.e. the GPK “fuel” shells). In the preparation of the concrete specimens the mix ratio was 1: 2: 4 (cement: sand: stone) by weight and the replacement percentage was 0%, 20%, 30%, 40%, 50% and 60% respectively. Concrete specimen were molded in both cubic and cylindrical form and its impact on the mechanical properties such as workability, compressive strength and flexural strength using destructive test method were studied. The cubic specimen were tested at 7, 28 and 60 days whiles the cylindrical specimen were tested at 7 and 28 days. Results of physical and chemical analyses suggest that GPK “fuel” shells have acceptable cementitious properties whiles GPK ordinary shells does not. Generally, the compressive and flexural strengths of concrete containing GPK shells decrease as the replacement percentage increases. However, the values of these properties increase as the period of curing increases. The optimum level of GPK shells replacement is 20% for the ordinary shells and 30% for the “fuel” shells considering compressive strength at 28 days for the cubic samples. For the flexural strength on the cylindrical specimen, up to 60% replacement of cement by GPK shells cured for 28 days has acceptable flexural strength. In spite of the findings that the GPK ordinary shells do not have cementitious properties, the mechanical properties on such concretes can be used in low strength constructions as pavements, walk ways and non structural domestic work at a lower cost than using cement.
Improving biogas production performance of dairy activated sludge via ultrasound disruption prior to microwave disintegration
Authors: Al Ramahi M. , Keszthelyi-Szabó G. , Beszédes S. 
Published: May 2020

Abstract
In this study, ultrasound disruption was employed to enhance the efficiency of microwave disintegration of dairy sludge. Results revealed that ultrasound specific energy input of 1,500 kJ/kg TS was found to be optimum with limited cell lysis at the end of the disruption phase. Biodegradability study suggested an enhancement in suspended solids reduction (16%) and biogas production (180 mL/gVS) in floc disrupted (deflocculated) samples when compared to sole microwave pretreatment (8.3% and 140 mL/gVS, respectively). Energy assessment to attain the 15% optimum solubilization revealed a positive net production of 26 kWh per kg sludge in deflocculated samples compared to 18 kWh in flocculated (sole microwave) samples. Thus, ultrasound disruption prior to microwave disintegration of dairy sludge was considered to be a feasible pretreatment technique.
Composición y distribución de la ictiofauna asociada a jagüeyes, en la región Caribe colombiana
Authors: Navarro Blas , Tovar Hernando , Caraballo Pedro 
Published: May 2020

Se estudiaron las comunidades de peces en 12 jagüeyes, en función del uso del suelo dado a la zona de captación (agrícola, ganadero y urbanístico), en los municipios de Magangué, Bolívar y Los Palmitos, Sucre. Se capturaron 5695 individuos pertenecientes a nueve familias y cinco órdenes y 13 especies: Hoplias malabaricus, Curimata magdalenae, Astianax fasciatus, Piaractus brachypomus, Roeboides dayii, Prochilodus magdalenae, Hoplosternum thoracatum, Hemiancistrus wilsoni, Caquetaia kraussii, Oreochromis spp., Andinoacara latifrons, Poecilia caucana, Synbranchus marmoratus. Los jagüeyes con mayor riqueza y abundancia fueron los de uso urbanístico con 11 especies y 2512 individuos, seguido del ganadero con siete especies y 2252 individuos, con aplicación de la Prueba F, no existe una diferencia estadísticamente significativa entre las medias de las tres variables con un nivel de confianza del 95,0 %, con un p= 0,54 para A. fasciatus y 0,47 para A. latifrons, p ≥ 0,05. El grado de recambio de especies (diversidad β) entre los jagüeyes y los tres usos dados a su zona de captación fue alto, porque la diferencia en la composición del ensamblaje de peces entre hábitat varía considerablemente, de 13 especies registradas compartieron entre dos y cuatro especies, con valores de complementariedad entre el 50 y 67 % (agricultura), 20 y 50 % (ganadería) y entre el 50 y 86 % (urbanístico), la similitud fue alta en los jagüeyes de uso ganadero. La riqueza y abundancia de peces en jagüeyes encontradas en este estudio tiene una gran significancia ecológica en la conservación y preservación de la diversidad biológica íctica en las sabanas y Bosque seco Tropical en los departamentos de Sucre y Bolívar muy a pesar de la presión ejercida por la contaminación orgánica e inorgánica, producida por las actividades: agrícola, ganadera y urbana.
Genetic Analysis of Pharmacogenomic VIP Variants of ABCB1, VDR and TPMT Genes in an Ethnically Isolated Population from the North Caucasus Living in Jordan
Authors: AL-Eitan Laith Naser , Al-Maqableh Haneen Waleed , Mohammad Namarg Nawwaf , Khair Hakooz Nancy Mohamed , Dajani Rana Basem 
Published: May 2020

Background:
Differences in individual responses to the same medications remarkably differ among
populations. A number of genes that play integral roles in drug responses have been designated as very important
pharmacogenes (VIP), as they are responsible for differences in drug safety, efficacy, and adverse drug reactions
among certain ethnic groups. Identifying the polymorphic distribution of VIP in a range of ethnic groups will be
conducive to population-based personalized medicine.

Objective:
The aim of the current study is to identify the polymorphic distribution of VIP regarding the Chechen
minority group from Jordan and compare their allele frequencies with other populations.

Methods:
A total of 131 unrelated Chechen individuals from Jordan were randomly recruited for blood collection.
Identification of allelic and genotypic frequencies of eleven VIP variants within the genes of interest (ABCB1, VDR
and TPMT) was carried out by means of the MassARRAY®System (iPLEX GOLD).

Results:
Within ABCB1, we found that the minor allele frequencies of the rs1128503 (A: 0.43), rs2032582 (A: 0.43),
rs1045642 (A: 0.43). For VDR, the minor allele frequencies of rs11568820 (T: 0.18), rs1540339 (T: 0.30), rs1544410
(T: 0.41), rs2228570 (T: 0.24), rs3782905 (C: 0.28) and rs7975232 (C: 0.45). Finally, the minor allele frequencies for
the TPMT rs1142345 and rs1800460 polymorphisms were found to be (C: 0.02) and (T: 0.01), respectively.

Conclusion:
Significant differences in allelic frequencies of eleven ABCB1, VDR and TPMT VIP variants were
found between Jordanian Chechens and other populations. In our study, most populations that are similar to
Chechens are those from South Asian, European (Finnish) and European, including: Utah residents with Northern
and Western European ancestry, Toscani in Italia, Mexican ancestry in Los Angeles and Circassian from Jordan. The
level of similarity between Chechens and those populations means that they might have shared high levels of gene
flow in the past. The results obtained in this study will contribute to the worldwide pharmacogenomic databases and
provide valuable information for future studies and better individualized treatments.
Role of Bacterial-Fungal Consortium for Enhancement in the Degradation of Industrial Dyes
Authors: Mawad Asmaa M.M. , El-Latif Hesham Abd , Yousef Naiema M.H. , Shoreit Ahmed A.M. , Gathergood Nicholas , Gupta Vijai Kumar 
Published: May 2020

Background:
The presence of anthraquinone (Disperse blue 64) and azodyes (Acid yellow
17) in a waterbody are considered among the most dangerous pollutants.

Methods:
In this study, two different isolated microbes, bacterium and fungus, were individually and
as a co-culture applied for the degradation of Disperse Blue 64 (DB 64) and Acid Yellow 17 (AY 17)
dyes. The isolates were genetically identified based upon 16S (for bacteria) and ITS/5.8S (for fungus)
rRNA genes sequences as Pseudomoans aeruginosa and Aspergillus flavus, respectively.

Results:
The fungal/bacterial consortium exhibited a higher percentage of dyes degradation than the
individual strains, even at a high concentration of 300 mg/L. Azoreductase could be identified as the
main catabolic enzyme and the consortium could induce azoreductase enzyme in the presence of both
dyes. However, the specific substrate which achieved the highest azoreductase specific activity was
Methyl red (MR) (3.5 U/mg protein). The tentatively proposed metabolites that were detected by
HPLC/MS suggested that the reduction process catalyzed the degradation of dyes. The metabolites
produced by the action consortium on two dyes were safe on Vicia faba and Triticum vulgaris germination
and health of seedlings. Toxicity of the dyes and their degradation products on the plant was
different according to the type and chemistry of these compounds as well as the type of irrigated
seeds.

Conclusion:
We submit that the effective microbial degradation of DB64 and AY17 dyes will lead to
safer metabolic products.
Synthesis, Characterization, Antimicrobial and Anticancer Studies of Metal Complexes of 2-methoxy-4-((3-methylpyridin-2-ylimino)methyl)phenol
Authors: Mishra Akanksha , Tahlan Sumit , Ramasamy Kalavathy , Lim Siong Meng , Shah Syed Adnan Ali , Narasimhan Balasubramanian 
Published: May 2020

Background:
Being derived from primary amine and aromatic aldehyde, Schiff base and
their complexes have an imperative role in the improvement of inorganic chemistry, which are broadly
studied as coordination compounds and are gradually becoming more important in biochemical and
analytical applications.

Methods:
They have also been used for antibacterial, antifungal, anticancer, antitubercular activities.
Novel synthesised Schiff’s base 2-methoxy-4-((3-methylpyridin-2-ylimino)methyl)phenol (SB) and its
metal complexes (Zn[II], Cu[II], Co[II] and Ni[II]) were characterised by UV, IR and NMR spectroscopy.
Formation of the Schiff base and the metal (Zn[II], Cu[II], Co[II] and Ni[II]) chelates was supported
by spectral and analytical data. The ligand and metal complexes have been screened for their
antibacterial activity against Staphylococcus aureus, Salmonella typhi, Escherichia coli, Klebsiella
pneumoniae and antifungal activity against the fungi Candida albicans and Aspergillus niger. Further,
the synthesised compounds were also screened for antiproliferative activity against the human colorectal
carcinoma (HCT116) cell line using the Sulforhodamine B assay.

Results:
Metal complexes formed were found to enhance the potency of the Schiff base due to coordination
with a copper complex, showing better activity than others.

Conclusion:
Copper complex was observed to be more potent than other complexes against all the
pathogenic microbes and cancer cell line (HCT116).
Real-time Polymerase Chain Reaction for Mycobacterium tuberculosis Meningitis is More Sensitive in Patients with HIV Co-Infection
Authors: de Almeida Sergio M. , Santana Lucas B. , Golin Gilberto , Kussen Gislene B. , Nogueira Keite 
Published: May 2020

Background:Tuberculous meningitis (TbM) is the most severe complication of extra pulmonary tuberculosis (Tb). There is a higher frequency of positive cerebrospinal fluid (CSF) cultures for Mycobacterium tuberculosis (MTb) in samples from human immunodeficiency virus (HIV) co-infected patients than in those from HIV-negative patients. We hypothesized that real time PCR assays for MTb (MTb qPCR) using CSF would be more sensitive in HIV co-infected patients owing to a greater MTb burden. The present study aimed to verify the diagnostic performance of MTb qPCR in CSF of TbM patients who either were co-infected with HIV or were HIVnegative.Methods:A total of 334 consecutive participants with suspected TbM were divided into two groups: HIV co-infected and HIV-negative; each group was categorized into definite TbM, probable TbM, possible TbM, and TbM-negative subgroups based on clinical, laboratory and imaging data. We evaluated the diagnostic characteristics of MTb qPCR analysis to detect TbM in CSF by comparing the results to those obtained for definite TbM (i.e., positive MTb culture) and/or probable TbM in CSF, as gold standard.Results:The sensitivity of MTb qPCR in the definite and probable subgroups of the HIV coinfected participants (n = 14) was 35.7%, with a specificity of 93.8%, negative predictive value (NPV) of 94.4%, and negative clinical utility index (CUI−) of 0.89. Results of the HIV-negative group (n = 7) showed lower sensitivity (14.3%) and similar specificity, NPV, and CUI−.Conclusion:The findings confirmed our hypothesis, despite the low sensitivity. MTb qPCR may significantly contribute to diagnosis when associated with clinical criteria and complementary examinations.
Prediction of Prophylactic Peptide Vaccine Candidates for Human Papillomavirus (HPV): Immunoinformatics and Reverse Vaccinology Approaches
Authors: Ismail Mehreen , Sajid Zureesha , Ali Amjad , Wu Xiaogang , Muhammad Syed Aun , Shaikh Rehan Sadiq 
Published: May 2020

Background:
Human Papillomavirus (HPV) is responsible for substantial morbidity and mortality worldwide. We predicted
immunogenic promiscuous monovalent and polyvalent T-cell epitopes from the polyprotein of the Human Papillomavirus (HPV) using a range
of bioinformatics tools and servers.

Methods:
We used immunoinformatics and reverse vaccinology-based approaches to design prophylactic
peptides by antigenicity analysis, T-cell epitopes prediction, proteasomal and conservancy
evaluation, host-pathogen protein interactions, and in silico binding affinity analysis.

Results:
We found two early proteins (E2 and E6) and two late proteins (L1 and L2) of HPV as potential vaccine candidates. Of these proteins
(E2, E6, L1 & L2), 2-epitopes of each candidate protein for multiple alleles of MHC class I and II bearing significant binding affinity (>-6.0
kcal/mole). These potential epitopes for CD4+ and CD8+ T-cells were also linked to design polyvalent construct using GPGPG linkers. Cholera
toxin B and mycobacterial heparin-binding hemagglutinin adjuvant with a molecular weight of 12.5 and 18.5 kDa were used for epitopes of
CD4+ and CD8+ T-cells respectively. The molecular docking indicated the optimum binding affinity of HPV peptides with MHC molecules.
This interaction showed that our predicted vaccine candidates are suitable to trigger the host immune system to prevent HPV infections.

Conclusion:
The predicted conserved T-cell epitopes would contribute to the imminent design of HPV vaccine candidates, which will be able
to induce a broad range of immune-responses in a heterogeneous HLA population.