bluuebunny/crossref_metadata_2025_split huggingface dataset:
Detection of a novel PAX6 mutation in a Chinese family with multiple ocular abnormalities
Authors: Ouyang Junyi , Cai Ziyan , Guo Yinjie , Nie Fen , Cao Mengdan , Duan Xuanchu 
Published: November 2020

Abstract
Background: Aniridia is a congenital, panocular disease affecting the cornea, anterior chamber angle, iris, lens, retina and optic nerve. PAX6 loss-of-function mutations were the most common cause of aniridia .Mutations throughout the PAX6 gene have been linked to a range of ophthalmic abnormalities, with distinct mutations at a given site within this gene leading to distinct phenotypic findings.This s tudy aimed to characterize genetic mutations associated with congenital aniridia in a Chinese family. Methods: The proband and the proband’s brother of this family underwent comprehensive ophthalmologic examinations as well as exome sequencing, with Next Generation Sequencing being used to confirm these results. Results: A novel mutation (c.114_119delinsAATTTCC:p.Pro39fs) in the PAX6 gene was identified in subjects III-2 and III-3 in these family, and both of these subjects exhibited complete aniridia, cataracts, glaucoma, high myopia, and foveal hypoplasia. Conclusions: We identified a novel PAX6 frameshift heterozygous deletion mutation in a Chinese family and determined that this mutation was a probable cause of various eye abnormalities in carriers.
Investigation on fluid flow heat transfer and frictional properties of Al2O3 nanofluids used in shell and tube heat exchanger
Authors: chandran sreejesh S R , Barik Debabrata , G ANSALAM RAJ T , ROY Reby 
Published: November 2020

Abstract
Nanofluids are generally utilized in providing cooling, lubrication phenomenon, controlling the thermophysical properties of the working fluid. In this work, nanoparticles of Al2O3 are added to the base fluid which flows through the counter flow arrangement in a turbulent flow condition. The hot and cold fluids used are ethylbenzene and water respectively and have different velocities on both shell and tube side. This study emphasizes the analysis of flow properties, friction loss, and energy transfer in terms of heat using nanofluid in the heat exchanger. The heat transfer rate of present investigation with nanoparticle addition is 4.63% higher in comparision to Dittus Boelter correlation. Apart from this, the obtained friction factor is 0.0376 very much closer to Gnielinski and Blasius correlations. This investigation proved that appropriate nanoparticle additions and baffle inclinations have fabulous impact upon the performance of heat exchanger and its effectiveness.
Development of Sustainable Phycoremediation with the help of Chlorella Pyrenoidosa for Textile Wastewater Analysis using Response Surface Methodology
Authors: Mishra Shubhangi , Srivast Pradeep , Singh Virendra , Sharma Monika 
Published: November 2020

Abstract
The uncontrolled utilization for the textile products is increasing year by year resulting with the elevating wastewater generated from the textile industries, which makes it among the prevalent sources of critical environmental deteoration issue globally. Products obtained from the dyes used are the primary toxic product for aquatic life, they cause aesthetic pollution, eutrophication, perturbation and increase in BOD and COD in aquatic life. Three types of textile wastewaters (Acid Yellow dye, Acid orange dye and Basic pink dye) has been used for wastewater treatment and microalgal (Chlorella pyrenoidosa) biomass production. Nitrogen content in textile wastewaters is very less, hence urea is used as nitrogen source in wastewater. Optimal growth condition (Urea-0.4g/L, wastewater- 40%(v/v)) is developed through Response surface methodology (RSM). The biomass productivity for chlorella sp. is 1.2-1.5 g/L/day in textile wastewaters. The reduction efficiency of COD, Nitrate-N Ammonia-N, Phosphate-P, and Dye(color) removal for Chlorella is 90-95%, 75-85%, 90-98%, 65-74% and 40-65%.After harvesting the Biomass by flocculation method it can be further used for biofuel production by in-situ transesterification.
Langit dan Bumi yang Baru: Eskatologi berdasarkan Teologi Biblika tentang Tempat Kediaman Allah
Authors: Yohanes Hendra 
Published: November 2020

Abstract
Popular Christian eschatology tends to nullify the earth from the biblical image of the new heaven and earth. On the contrary, the biblical eschatology shows that the new heaven and earth will be the dwelling place of God who will ultimately be with the redeemed people. Using the method of intertextual exegesis, this study constructs a biblical eschatology in terms of the theme of the dwelling place of God that frames the biblical portraits of Eden in the first creation and the New Jerusalem in the new heaven and earth. Sinful humankind has been expelled from Eden, the first sanctuary, yet the New Jerusalem will be the holy dwelling place of God, where God’s people enjoying eternal life. The new heaven and new earth is the telos of God’s salvation plan where no sin defiles the cosmic temple anymore. The biblical eschatological hope promises God’s people about living as the royal priests on the earth that God will renew at the eschaton.
Abstrak
Eskatologi Kristen populer cenderung mengecualikan bumi dari gambaran alkitabiah langit dan bumi yang baru. Sebaliknya, eskatologi biblika menunjukkan bahwa langit dan bumi yang baru merupakan tempat kediaman Allah yang akhirnya akan bersama dengan umat tebusan-Nya. Penulis menggunakan metode eksegesis intertekstual untuk mengonstruksi eskatologi biblika berdasarkan tema tempat kediaman Allah yang membingkai gambaran-gambaran Alkitab, yakni Eden di dunia ciptaan yang pertama dan Yerusalem Baru di langit dan bumi yang baru. Manusia yang berdosa telah diusir dari Eden, tempat kudus yang pertama. Namun Yerusalem Baru merupakan tempat kediaman Allah yang kudus, di mana umat Allah akan menikmati kehidupan kekal. Langit dan bumi yang baru merupakan tujuan rencana keselamatan Allah, di mana tiada lagi dosa yang menajiskan bait semesta. Pengharapan eskatologis yang alkitabiah menjanjikan kehidupan umat Tuhan sebagai imamat rajawi di bumi yang akan dibarui oleh Allah pada eschaton.
Pesan Suci yang Terkontaminasi: Suatu Tinjauan atas Pendekatan Pascakolonial Sugirtharajah dan Konteks Indonesia
Authors: Hutabarat Haleluya Timbo 
Published: November 2020

Abstract
The history of religions records the existence of persistent violence in religions. Many rulers, with the help of clergies, misuse sacred texts for their conquering interests. The coming of Christianity to Indonesia was linked to Western colonialism with its exploitating ambition. Todate, the fruit of the agenda of misusing Scriptures can still be found in the theology and traditions of the Indonesian churches. This study presents the post-colonial biblical criticism of Sugirtharajah as an inclusive, collaborative hermeneutic umbrella for efforts to liberatetexts, traditions, and contexts of Indonesia. Rasiah S. Sugirtharajah has pioneered the post-colonial biblical criticism as a hermeneutics that criticizes domination and alienation. This study looks at the relevance of Sugirtharajah’s thinking for the context of Indonesian Christianity. The methods used include qualitative literature review on the postcolonialpublication in Indonesia to find out the progress of the existing post-colonial hermeneutic works.
Abstrak
Sejarah agama-agama mencatat hadirnya kekerasan secara persisten. Penguasa, dengan bantuan rohaniwan, sering kali menyisipkan kepentingan penaklukannya ke dalam penggunaan ayat-ayat suci. Kekristenan di Indonesia datang berkaitan dengan kolonialisme Barat dengan ambisi eksploitatifnya. Dalam hal itu terjadi juga kolaborasi saling menguntungkan antara misionaris dan penguasa (ekonomi dan militer) kolonial. Produk agenda penundukan dan pembodohan yang menggunakan ayat-ayat Kitab Suci masih terasa dalam teologi dan tradisi gereja Indonesia hingga sekarang. Bentuk kolonialismebaru juga terus bermekaran di dalam dan sekitar gereja. Studi ini menyelidiki pendekatanhermeneutik yang dapat melawan upaya mengkontaminasi Kitab Suci. Studi ini menyuguhkan Kritik Alkitabiah Pascakolonial Sugirtharajah sebagai payung hermeneutis kolaboratif inklusif bagi banyak upaya membebaskan teks, tradisi, dan konteks. Metode yang dipakai adalah analisis historis mengikuti kerangka teori Sugirtharajah. Juga dilakukan tinjauan literatur terhadap buku-buku teologi bernafas pascakolonial yang banyak dipakai di Indonesia guna melihat sejauh mana upaya pascakolonial telah ada sekaligus perlu dikembangkan sesuai pemikiran Sugirtharajah. Hasil studi ini diharapkan bisa membantu kekristenan Indonesia untuk lebih merdeka dan terampil dalam membebaskan teks, teologi, tradisi, dan penafsiran Alkitab secara pascakolonial berdasarkan konteks semesta dan manusia Indonesia.
Sustainability requirements of implementing water-reuse concepts for new industrial park developments in water-stressed regions
Authors: Bauer S. , Dell A. , Behnisch J. , Linke H. J. , Wagner M. 
Published: November 2020

Abstract
Requirements for wastewater management and water-reuse concepts concerning sustainability are gaining greater importance, especially in times of climate change. Industrial park developments are often hindered due to water scarcity. Thus, nowadays, the reuse of wastewater is becoming more and more important to increase the availability of water and to enable new developments. The sustainable Industrial WasteWater Management Concept with the focus on Reuse (IW2MC → R) provides a solution strategy to produce reuse water from industrial wastewater within production plants. To achieve sustainability, certain requirements are essential since the reuse water can be provided directly via an optimized wastewater treatment process for subsequent use. Hence, it is then ‘fit for purpose’. This enables a more efficient application of reuse water. Furthermore, due to environmental issues, it is important to construct space-saving water resource recovery facilities for reducing land consumption within industrial parks.
Hydraulic jump on rough beds: conceptual modeling and experimental validation
Authors: Türker Umut , Valyrakis Manousos 
Published: November 2020

Abstract
The aim of this study was to assess and quantify the effect of channel bed roughness on hydraulic jumps based on sound physical theories. Assuming that integrated bed shear stress due to surface roughness changes linearly with supercritical velocity, a novel definition for the shear force coefficient and for roller length were obtained. Experimental findings and Pearson's correlation verify that the developed equations perform reasonably well and they prove that a linear correlation assumption between integrated bed shear stress and supercritical velocity is valid for a Froude number between 1.1 and 9.8. The shear force coefficient is defined in terms of the Reynolds coefficient and the supercritical flow velocity is directly related to the modified Reynolds number. A new analytical equation for roller length as a function of the modified Reynolds number was also developed and validated by using data from the experimental study.
A characteristic study of Zea mays L. (sweet corn) cobs for synthetic dye degradation from aqueous media
Authors: Javed Iram , Javed Tariq , Khan Muhammad Naeem 
Published: November 2020

Abstract
The current study reports a systematic methodology of Zea mays L. (sweet corn) cobs (ZMLC) for the sequestration of synthetic dye (gentian violet) from aqueous solutions. Adsorbent was scrutinized by using scanning electron microscopy, Fourier transform infra-red spectrometry with pHpzc determination. The impact of various adsorption parameters including pH effect, ZMLC (sorbent) dosage, temperature, concentration and shaking time was examined. The equilibrium sorption isotherms were determined by the batch method from 283 to 303 K at pHpzc. Adsorption data were adjusted to four isothermal models: Langmuir, Freundlich, Dubinin-Radushkevich and Temkin's models, which presented the best adjustment to Freundlich, Dubinin-Radushkevich and Temkin's at 283 K. The kinetic profile fitted well to the pseudo-second order kinetic equation at three distinct concentrations 600, 700, 800 mg/L. Maximum sorption capacity was gained up to 700 mg.g−1 for gentian violet at pH 3, respectively. The adsorption process is endothermic, non-spontaneous, favorable thermodynamically due to positive values of entropy and Gibbs free energy and randomness decreases during the adsorption process. Furthermore, after biosorption onto ZMLC the dye can be desorbed effectively by using mineral base KOH solution. Consequently, the ZMLC is said to be a promising biosorbent to remediate gentian violet-contaminated water as well as wastewater.
Bacterial community dynamics in tropical soil after sewage sludge amendment
Authors: Lopes Bruna C. , Figueiredo Raíssa S. , Araújo Juliana C. , Matos Antonio T. 
Published: November 2020

AbstractAlthough the widespread use of sewage sludge in developing countries is common, little is known about how sludge disposal can affect the microbial composition and diversity of tropical soils. We evaluated the effects of the sewage sludges of two types of anaerobic digestors differing, by the biological treatment they have undergone (uplow anaerobic sludge blanket and activated sludge digester), and two different disposal methods (surface and incorporated) on tropical soils. Samples were taken from topsoil (0–10 cm) and analyzed by amplifying the 16S rRNA genes to study the microbial community, and physicochemical analysis was performed concomitantly. The results indicated that, in general, sewage sludge amendment (SSA) significantly changed the tropical soil bacterial community by the sludge type and by application method. Moreover, the redundancy analysis diagram indicates that changes in soil chemical parameters over time due to SSA resulted in changes in the bacterial community's composition, increasing the population responsible for recycling nutrients in the soil.
New Insights on Fak and Fak Inhibitors
Authors: Brullo Chiara , Tasso Bruno 
Published: November 2020

Background:
Focal adhesion kinase (Fak) is a cytoplasmic protein tyrosine kinase
overexpressed and activated in different solid cancers; it has shown an important
role in metastasis formation, cell migration, invasion and angiogenesis and consequently
it has been proposed as a potential target in cancer therapy, particularly in a metastatic
phase. In recent years, different investigations have highlighted the importance of new
Fak inhibitors as potential anti-cancer drugs, but other studies evidenced its role in different
pathologies related to the cardiac function or viral infection.

Methods:
An extensive bibliographic research (104 references) has been done concerning
the structure of Fak, its importance in tumor development, but also in other pathologies
currently under study. The compounds currently subjected to clinical studies were
therefore treated using the appropriate databases. Finally, the main chemical scaffolds
currently under preclinical investigation were analyzed, focusing on their molecular structures
and on the activity structure relationships (SAR).

Results:
At the moment, only a few reversible ATP-competitive inhibitors are under investigation
in pre-clinical studies and clinical trials. Other compounds, with different
chemical scaffolds, are investigated to obtain more active and selective Fak inhibitors.
This mini-review is a summary of different Fak functions in cancer and other pathologies;
the compounds today in clinical trials and the recent chemical scaffolds (also included
in patents) giving the most interesting results are investigated. In addition, PROTAC
molecules are reported.

Conclusion:
All reported results evidenced that additional studies are necessary to design
and synthesize new selective and more active compounds, although promising information
has been obtained from associations between Fak inhibitors and other different anti-
cancer drugs. In addition, the other important roles evidenced, both at the nuclear level
and in non-cancerous cells, make this protein an increasingly important target in pharmaceutical
chemistry.
Characterization of the Chloroplast Genome Facilitated the Transformation of Parachlorella kessleri-I, A Potential Marine Alga for Biofuel Production
Authors: Nawkarkar Prachi , Chugh Sagrika , Sharma Surbhi , Jain Mukesh , Kajla Sachin , Kumar Shashi 
Published: November 2020

Introduction:
The microalga Parachlorella kessleri-I produces high biomass and lipid content
that could be suitable for producing economically viable biofuel at a commercial scale. Sequencing
the complete chloroplast genome is crucial for the construction of a species-specific chloroplast
transformation vector.

Methods:
In this study, the complete chloroplast genome sequence (cpDNA) of P. kessleri-I was assembled;
annotated and genetic transformation of the chloroplast was optimized. For the chloroplast
transformation, we have tested two antibiotic resistance makers, aminoglycoside adenine transferase
(aadA) gene and Sh-ble gene conferring resistance to spectinomycin and zeocin, respectively.
Transgene integration and homoplasty determination were confirmed using PCR, Southern blot and
Droplet Digital PCR.

Results:
The chloroplast genome (109,642 bp) exhibited a quadripartite structure with two reverse repeat
regions (IRA and IRB), a long single copy (LSC), and a small single copy (SSC) region. The genome
encodes 116 genes, with 80 protein-coding genes, 32 tRNAs and 4 rRNAs. The cpDNA provided
essential information like codons, UTRs and flank sequences for homologous recombination to
make a species-specific vector that facilitated the transformation of P. kessleri-I chloroplast. The
transgenic algal colonies were retrieved on a TAP medium containing 400 mg. L-1 spectinomycin, but
no transgenic was recovered on the zeocin-supplemented medium. PCR and Southern blot analysis ascertained
the transgene integration into the chloroplast genome, via homologous recombination. The
chloroplast genome copy number in wildtype and transgenic P. kessleri-I was determined using Droplet
Digital PCR.

Conclusion:
The optimization of stable chloroplast transformation in marine alga P. kessleri-I should
open a gateway for directly engineering the strain for carbon concentration mechanisms to fix more
CO2, improving the photosynthetic efficiency and reducing the overall biofuels production cost.
Genome-wide Identification, Evolution and Expression Analysis of Basic Helix-loop-helix (bHLH) Gene Family in Barley (Hordeum vulgare L.)
Authors: Ke Qinglin , Tao Wenjing , Li Tingting , Pan Wenqiu , Chen Xiaoyun , Wu Xiaoyu , Nie Xiaojun , Cui Licao 
Published: November 2020

Background:
The basic helix-loop-helix (bHLH) transcription factor is one of the most important
gene families in plants, playing a key role in diverse metabolic, physiological, and developmental
processes. Although it has been well characterized in many plants, the significance of the
bHLH family in barley is not well understood at present.

Methods:
Through a genome-wide search against the updated barley reference genome, the genomic
organization, evolution and expression of the bHLH family in barley were systematically analyzed.

Results:
We identified 141 bHLHs in the barley genome (HvbHLHs) and further classified them into
24 subfamilies based on phylogenetic analysis. It was found that HvbHLHs in the same subfamily
shared a similar conserved motif composition and exon-intron structures. Chromosome distribution
and gene duplication analysis revealed that segmental duplication mainly contributed to the expansion
of HvbHLHs and the duplicated genes were subjected to strong purifying selection. Furthermore, expression
analysis revealed that HvbHLHs were widely expressed in different tissues and also involved
in response to diverse abiotic stresses. The co-expression network was further analyzed to underpin
the regulatory function of HvbHLHs. Finally, 25 genes were selected for qRT-PCR validation, the expression
profiles of HvbHLHs showed diverse patterns, demonstrating their potential roles in relation
to stress tolerance regulation.

Conclusion:
This study reported the genome organization, evolutionary characteristics and expression
profile of the bHLH family in barley, which not only provide the targets for further functional analysis,
but also facilitate better understanding of the regulatory network bHLH genes involved in stress
tolerance in barley.
Treatment Strategies Against Diabetic Foot Ulcer: Success so Far and the Road Ahead
Authors: Awasthi Ankit , Singh Sachin Kumar , Kumar Bimlesh , Gulati Monica , Kumar Rajesh , Wadhwa Sheetu , Khursheed Rubiya , Corrie Leander , KR Arya , Kumar Rajan , Patni Pooja , Kaur Jaskiran , Vishwas Sukriti , Yadav Ankit 
Published: November 2020

Background:
Diabetic foot ulcer (DFU) is one of the leading complications of type-2 diabetes
mellitus. It is associated with neuropathy and peripheral arterial disease of the lower limb in
patients with diabetes. There are four stages of wound healing, namely hemostasis phase, inflammatory
phase, proliferative phase and maturation phase. In the case of DFU, all these stages are disturbed
which lead to delay in healing and consequently to lower limb amputation. Conventional
dosage forms like tablets, creams, ointments, gels and capsules have been used for the treatment of
diabetic foot ulcer for many years.

Introduction:
In this review, the global prevalence as well as etiopathogenesis related to diabetic
foot ulcer have been discussed. The potential role of various synthetic and herbal drugs, as well as
their conventional dosage forms in the effective management of DFU have been discussed in detail.

Methods:
Structured search of bibliographic databases from previously published peer-reviewed research
papers was explored and data has been represented in terms of various approaches that are
used for the treatment of DFU.

Results:
About 148 papers, including both research and review articles, were included in this review
to produce a comprehensive as well as a readily understandable article. A series of herbal and
synthetic drugs have been discussed along with their current status of treatment in terms of dose
and mechanism of action.

Conclusion:
DFU has become one of the most common complications in patients having diabetes
for more than ten years. Hence, understanding the root cause and its successful treatment is a big
challenge because it depends upon multiple factors such as the judicious selection of drugs as well
as proper control of blood sugar level. Most of the drugs that have been used so far either belong to
the category of antibiotics, antihyperglycaemic or they have been repositioned. In clinical practice,
much focus has been given to dressings that have been used to cover the ulcer. The complete treatment
of DFU is still a farfetched dream to be achieved and it is expected that combination therapy
of herbal and synthetic drugs with multiple treatment pathways could be able to offer better management
of DFU.