bluuebunny/crossref_metadata_2025_split huggingface dataset:
TRTools: a toolkit for genome-wide analysis of tandem repeats
Authors: Mousavi Nima , Margoliash Jonathan , Pusarla Neha , Saini Shubham , Yanicky Richard , Gymrek Melissa 
Published: March 2020

AbstractSummaryA rich set of tools have recently been developed for performing genome-wide genotyping of tandem repeats (TRs). However, standardized tools for downstream analysis of these results are lacking. To facilitate TR analysis applications, we present TRTools, a Python library and a suite of command-line tools for filtering, merging, and quality control of TR genotype files. TRTools utilizes an internal harmonization module making it compatible with outputs from a wide range of TR genotypers.AvailabilityTRTools is freely available athttps://github.com/gymreklab/TRTools.Contactmgymrek@eng.ucsd.eduSupplementary informationSupplementary data are available atbioRxiv.
Expanding the chemical space of synthetic cyclic peptides using a promiscuous macrocyclase from prenylagaramide biosynthesis
Authors: Sarkar Snigdha , Gu Wenjia , Schmidt Eric W. 
Published: March 2020

ABSTRACTCyclic peptides are excellent drug candidates, placing macrocyclization reactions at the apex of drug development. PatG and related dual-action proteases from cyanobactin biosynthesis are responsible for cleaving off the C-terminal recognition sequence and macrocyclizing the substrate to provide cyclic peptides. This reaction has found use in the enzymatic synthesis of diverse macrocycles. However, these enzymes function best on substrates that terminate with the non-proteinogenic thiazole/thiazoline residue, complicating synthetic strategies. Here, we biochemically characterize a new class of PatG-like macrocyclases that natively use proline, obviating the necessity of additional chemical or biochemical steps. We experimentally define the biochemical steps involved in synthesizing the widespread prenylagaramide-like natural products, including macrocyclization and prenylation. Using saturation mutagenesis, we show that macrocyclase PagG and prenyltransferase PagF are highly promiscuous, producing a library of more than 100 cyclic peptides and their prenylated derivativesin vitro. By comparing our results to known cyanobactin macrocyclase enzymes, we catalog a series of enzymes that collectively should synthesize most small macrocycles. Collectively, these data reveal that, by selecting the right cyanobactin macrocyclase, a large array of enzymatically synthesized macrocycles are accessible.
An 1,4-α-glucosyltransferase defines a new maltodextrin catabolism scheme inLactobacillus acidophilus
Authors: Andersen Susan , Møller Marie S. , Poulsen Jens-Christian N. , Pichler Michael J. , Svensson Birte , Goh Yong Jun , Leggio Leila Lo , Hachem Maher Abou 
Published: March 2020

ABSTRACTThe maltooligosaccharide (MOS) utilization locus inLactobacillus acidophilusNCFM, a model for human small-intestine lactobacilli, encodes a family 13 subfamily 31 glycoside hydrolase (GH13_31), annotated as an 1,6-α-glucosidase. Here, we reveal that this enzyme (LaGH13_31B) is an 1,4-α-glucosyltransferase that disproportionates MOS with preference for maltotriose.LaGH13_31B acts in concert with a maltogenic α-amylase that efficiently releases maltose from MOS larger than maltotriose. Collectively, these two enzymes promote efficient conversion of preferentially odd-numbered MOS to maltose that is phosphorolysed by a maltose phosphorylase, encoded by the same locus. Structural analyses revealed the presence of a flexible elongated loop, which is unique forLaGH13_31B and its close homologues. The identified loop insertion harbours a conserved aromatic residue that modulates the activity and substrate affinity of the enzyme, thereby offering a functional signature of this previously undescribed clade, which segregates from described activities such as 1,6-α-glucosidases and sucrose isomerases within GH13_31. Sequence analyses revealed that theLaGH13_31B gene is conserved in the MOS utilization loci of lactobacilli, including acidophilus cluster members that dominate the human small intestine.IMPORTANCEThe degradation of starch in the small intestine generates short linear and branched α-glucans. The latter are poorly digestible by humans, rendering them available to the gut microbiotae.g. lactobacilli adapted to the human small intestine and considered as beneficial to health. This study unveils a previously unknown scheme of maltooligosaccharide (MOS) catabolism, via the concerted action of activity together with a classical hydrolase and a phosphorylase. The intriguing involvement of a glucosyltransferase is likely to allow fine-tuning the regulation of MOS catabolism for optimal harnessing of this key metabolic resource in the human small intestine. The study extends the suite of specificities that have been identified in GH13_31 and highlights amino acid signatures underpinning the evolution of 1,4-α-glucosyl transferases that have been recruited in the MOS catabolism pathway in lactobacilli.
Hybrid quantum dot - collagen extracellular matrices for in situ optical monitoring of cardiomyocyte activity by two-photon fluorescence
Authors: Jooken Stijn , de Coene Yovan , Deschaume Olivier , Krylychkina Olga , Verbiest Thierry , Clays Koen , Callewaert Geert , Bartic Carmen 
Published: March 2020

ABSTRACTThe incorporation of functional nanoparticles in scaffolds for tissue constructs has led to the creation of artificial extracellular matrices that more accurately mimic the cues present in the native microenvironment of developing tissue. Additionally, light-sensitive inorganic nanoparticles can act as cell biosensors and report on the physiological parameters during tissue growth and organization. In this work, we functionalized collagen nanofibers with semiconductor quantum dots (QDs) and thereby created artificial extracellular matrices that can optically report on cardiomyocyte activity based on QD two-photon fluorescence. We have applied these optically-addressable nanofiber matrices to monitor activities of primary cardiomyocytes and compared the optical responses with patch-clamp data. Combining the long-term stability of QD fluorescence with the deeper light penetration depths achievable through multiphoton imaging, this approach can be used for continuous monitoring of cellular functions in cardiac tissue engineering.Abstract FigureConcept illustration: optical readout of cardiomyocyte activity with QD-functionalized collagen networks. Whole-cell current-clamp mode is used here to simultaneously monitor changes in the transmembrane voltage while the QD two-photon fluorescence is recorded.
Revisiting dynamic capability for organizations' innovation types
Authors: Ilmudeen Aboobucker , Bao Yukun , Alharbi Ibraheem Mubarak , Zubair Nawaz 
Published: March 2020

PurposeDespite the existing literature on the impact of IT capability and innovation capabilities, this study examines how IT-enabled dynamic capability dimensions impact on firm innovative capability to achieve organizational performance.Design/methodology/approachDrawing on the dynamic capability theory, this study empirically investigates the entire chain of relationships among dynamic capability, innovative capability, organizational performance and turbulent environment.FindingsUsing the data from 254 Chinese firms, this study reveals IT-enabled dynamic capability dimensions have positive and significant relationship with firm innovative capability types, which in turn have significant relationship with organizational performance except the process innovation.Research limitations/implicationsThis study contributes to the growing information systems literature and also suggests theoretical and practical implications.Originality/valueThis study examines IT-enabled dynamic capability with firm innovative capability types, which has received limited attention in the past.
Assessment of information literacy skills
Authors: Okeji Chukwuma Clement , Ilika Obiageli Martina , Baro Emmanuel Ebikabowei 
Published: March 2020

Purpose
The purpose of this study is to assess information literacy skills of undergraduates in Nigerian universities.

Design/methodology/approach
A self-assessed questionnaire was developed to collect data from the final year undergraduates in 15 universities. A total of 1,350 final year Library and Information Science (LIS) students responded to the questionnaire which was used for analysis.

Findings
The study revealed that the majority of the LIS final year undergraduates mostly consult journal articles, followed by internet information and online databases during their research process. The undergraduates rated well-known author(s), current information, credible information, accurate and relevant information as very important when evaluating online information resources. It was found that the students rated their ability to organize information and integrate ideas from consulted information as high. While they were deficient in ability to correctly paraphrase ideas to avoid plagiarism and use citation and referencing styles correctly. On the whole, almost half of the final year undergraduates of LIS rated their level of information literacy skills to be moderate.

Practical implications
The findings of the study will inform librarians and faculty in universities in developing countries like Nigeria to develop an information literacy instruction policy.

Originality/value
Assessing information literacy skills will provide the foundation and knowledge to improve the development of information literacy training programmes.
How mindset and store familiarity impact online stockout responses
Authors: Gunness Aneeshta , Oppewal Harmen 
Published: March 2020

PurposeEffects of stockouts on purchase decisions have been examined from a variety of perspectives; little is yet known about how consumers react to stockouts in online shopping contexts. The present study investigates how stockout reactions depend on a consumer's mindset and familiarity with a website and investigates the role of negative affect in determining a consumer's stockout reaction.Design/methodology/approachShopping mindsets (deliberative vs. implemental) and website familiarity (high vs. low) were manipulated in an online experiment consisting of a simulated shopping task at an existing website which next was presented as having a stockout. The study observed the participants' switching responses and measured their negative affect.FindingsFindings indicate that when encountering an online stockout, consumers in an implemental mindset are more likely to switch away from the website than those in a deliberative mindset and are more likely to search for additional items at a competing site. Consumers who are more familiar with the website where they encounter the stockout display a higher likelihood of defecting to a competing site; however, when they are in an implemental mindset, their inclination to defect decreases. The study also shows that the strength of negative emotions affects OOS responses in that buyers that experience more negative emotions are more likely to defect from the site.Practical implicationsThe study's findings provide suggestions as to how retailers can manage and minimize defection behaviours associated with online stockouts. In designing operational and marketing strategies retailers need to pay close attention to how consumers' individual mindsets may vary by trait or circumstance and how they hence may respond differently to stockouts.Originality/valueThe authors introduce a novel perspective to the literature on stockout induced reactions and contribute by furthering investigation into previously unexplored specific consumer characteristics and intricacies of stockouts that drive particular stockout reactions.
Governance and customer value creation in business solutions
Authors: Mooi Erik , Kashyap Vishal , van Aken Marc 
Published: March 2020

PurposeThis paper aims to consider the impact of contractual and normative governance mechanisms on recommendation intent in a context of healthcare and professional lighting where repeat business from a customer is absent. The authors suggest both contractual and normative governance can create recommendation intent, but only when sufficient customer value is created.Design/methodology/approachThe authors draw on a combination of survey and archival data from the supplier and customer in the medical equipment and advanced (business) lighting systems industries. The authors analyze the data using seemingly unrelated regression and mediation tests.FindingsContracts and relational norms can increase customer recommendation intent, but only when the supplier creates customer value.Practical implicationsThe paper’s findings suggest that customers of business solutions are more likely to recommend their supplier when contracts are relatively detailed and when buyers and suppliers attempt to craft strong relational norms, despite service solutions being delivered during a relatively short time span.Originality/valueThe extant research on business solutions has focused on extended relationships between exchange partners with a high likelihood of repeated transactions. The authors demonstrate how to govern relationships in a solutions context where the likelihood of repeat business from the same customer is low using contractual and normative governance.
Quantifying the sustainability of Bitcoin and Blockchain
Authors: Fry John , Serbera Jean-Philippe 
Published: March 2020

PurposeThe authors develop new quantitative methods to estimate the level of speculation and long-term sustainability of Bitcoin and Blockchain.Design/methodology/approachThe authors explore the practical application of speculative bubble models to cryptocurrencies. They then show how the approach can be extended to provide estimated brand values using data from Google Trends.FindingsThe authors confirm previous findings of speculative bubbles in cryptocurrency markets. Relatedly, Google searches for cryptocurrencies seem to be primarily driven by recent price rises. Overall results are sufficient to question the long-term sustainability of Bitcoin with the suggestion that Ethereum, Bitcoin Cash and Ripple may all enjoy technical advantages relative to Bitcoin. Our results also demonstrate that Blockchain has a distinct value and identity beyond cryptocurrencies – providing foundational support for the second generation of academic work on Blockchain. However, a relatively low estimated long-term growth rate suggests that the benefits of Blockchain may take a long time to be fully realised.Originality/valueThe authors contribute to an emerging academic literature on Blockchain and to a more established literature exploring the use of Google data within business analytics. Their original contribution is to quantify the business value of Blockchain and related technologies using Google Trends
Exploring career choices of Emirati women in the technology sector
Authors: Howe-Walsh Liza , Turnbull Sarah , Khan Saleena , Pereira Vijay 
Published: March 2020

PurposeThe study aims to explore the factors that influence Emirati women's career choice in the UAE. This study contributes to the influence of context in career choices by investigating how Emirati women chose information technology (IT) as a profession through the lens of the social cognitive career theory.Design/methodology/approachThis study undertook in-depth interviews with 21 Emirati women working in technology in the UAE. The study considers women's career choices at three levels, i.e. from an individual, organisational and national context perspective.FindingsThe key findings include identifying the importance of national context in influencing career choices among other factors such as family centrality, desire to be seen as a role model, company reputation and government policy.Practical implicationsThe study has wider implications for women's career choices in other contexts. The findings highlight the challenges women face, such as a lack of role models and family centrality, which need to be considered in recruitment policies and practices in other national contexts.Originality/valueThe originality of the study is its contribution to the literature developing understanding of the influences on women's career choices in the Emirates. While previous studies have identified the role of patriarchal influence on women's careers, we have less understanding of the importance attributed to individual factors such as being perceived as a role model within their family and to society. Similarly, the literature provides limited evidence of the influence of factors such as government sponsorship and company reputation.
Exchange-traded fund investing as European open-end diversified core equity real-estate funds' cash substitute
Authors: Jadevicius Arvydas 
Published: March 2020

PurposeThe study is set to explore a viability for substituting part of cash holdings within European open-end diversified core equity (ODCE) real-estate funds with listed real-estate exchange-traded fund (ETF) alternative. Academically, this research bridges a knowledge gap within private real-estate market research.Design/methodology/approachFirst, the study investigates the correlation between ODCE and ETFs to assess series interdependence. Next, the study generates a blended ODCE and ETF portfolio and examines its performance by quantifying a) the contribution to returns and b) the diversification benefits.FindingsThe findings suggest that a 1 percent spare cash allocation to an ETF increases ODCE fund returns by few bps although the diversification benefits are more nuanced.Practical implicationsReal estate and other investment vehicles are encouraged to review their cash-holding strategies. Real estate, infrastructure or private equity vehicles could designate a small proportion of available cash to asset class-specific ETFs. These cash substitutes are likely to increase returns and could strengthen diversification, although there are some caveats. For ESG-conscious investors, sustainable ETFs and associated passive conduits with strong responsible investment characteristics could provide cash replacement alternatives at the margin.Originality/valueThe study adds additional evidence on the contested issue of blending private and public real estate.
Entrepreneurial reentry after failure: a review and future research agenda
Authors: Tipu Syed Awais Ahmad 
Published: March 2020

PurposeThis paper aims to review the academic literature on entrepreneurial reentry after failure in an attempt to highlight the contribution to the knowledge, identify research gaps and outline an agenda for future research.Design/methodology/approachSeveral databases such as ABI/Inform Global, Academic Search Complete, Business Source Premier and Emerald Full Text were used to find peer-reviewed journal articles. Different search terms were used, such as entrepreneurial reentry, failure of habitual entrepreneurs, reentry intentions, entrepreneurial failure, serial entrepreneurship and venture failure. A total of 27 articles were finally selected and included in the final analysis. Using thematic codes, the selected articles were manually coded.FindingsThe concept of entrepreneurial reentry after failure has recently gained some attention from entrepreneurship scholars, but still, there are significant gaps in the literature. A wide range of entrepreneurship theories can potentially provide the necessary impetus to guide future research. The current literature remains largely inconclusive with inconsistent findings. This underlines the need to focus on this domain to conduct more studies to develop knowledge. The available literature is largely focused on exploring antecedents of entrepreneurial reentry after failure. Therefore, the author’s understanding remains limited with regard to other aspects of entrepreneurial reentry after failure, such as context of reentry and outcomes of reentry. Moreover, future studies also need to include the developing country context for better understanding of entrepreneurial reentry after failure.Originality/valueTo the best of the author's knowledge, the current paper is the first identifiable review of the literature on entrepreneurial reentry after failure. The suggested areas of future research will potentially help in addressing the identified research gaps and further strengthening the theoretical foundations of this emerging research domain. Identified themes in the literature will also potentially help aspiring entrepreneurs to better understand the antecedents, contextual settings and outcomes of reentry after failure. This practical perspective will help failed entrepreneurs in particular to be more aware of the dynamics of reentry after failure and better manage the reentry process.
Reconsidering concept of knowledge sharing: search for quality dimensions
Authors: Doronin Dmitrii , Lei Shen , Shah Syed Hamad Hassan 
Published: March 2020

Purpose
Importance of knowledge sharing is undeniable; however, a number of studies have shown it is not always beneficial. The purpose of this study is to breakdown existing definitions of knowledge sharing to understand what elements of it might be considered in quality dimension measures and based on those synthesize a more comprehensive definition.

Design/methodology/approach
Through bibliometric investigation, the most influential definitions were investigated and the concept of knowledge sharing was redefined according to a process structure with the help of Grounded Theory.

Findings
The research has revealed the dual nature of knowledge sharing, that is, knowledge as input and sharing as a process. Related quality measures were proposed.

Originality/value
This study opens up a basis for future research of how companies and organization can improve the benefits of knowledge sharing by paying attention to its quality rather than quantity.
Exploring the influence of CEO and chief diversity officers' relational demography on organizational diversity management
Authors: Ng Eddy S. , Sears Greg J. , Arnold Kara A. 
Published: March 2020

PurposeDrawing on the relational demography literature and a social identity perspective, several research propositions in which the authors postulate that demographic characteristics (e.g. gender and race) of senior leaders will influence the implementation and effectiveness of diversity management practices were presented. Specifically, the authors focus on the Chief Executive Officer/Chief Diversity Officer (CEO/CDO) dyad and explore independent and joint effects of CEO and CDO majority–minority group status on workplace diversity outcomes, outlining key identity-based and relational moderators (e.g. value threat, relational identity and leader–member exchange) of these relationships.Design/methodology/approachThe literature on relational demography and leader–member exchange to develop propositions for future research was integrated.FindingsThis is a conceptual paper. There is no empirical data reported testing the propositions.Research limitations/implicationsThe authors extended theory and research on relational demography by focusing on senior leaders in the organization and proposing that the influence of CEO and CDO demographic characteristics on the enactment of diversity practices may be contingent on key identity-based and relational processes.Originality/valueThe authors are not aware of any studies investigating how personal characteristics and relational processes relating to the CEO and CDO may influence the implementation and effectiveness of workplace diversity management practices. In a similar vein, the authors contribute to the research literatures on relational demography and social identity by extending the application of these theories to senior leaders in organizations and in relation to the work of CEOs and CDOs.