bluuebunny/crossref_metadata_2025_split huggingface dataset:
Satellite Gravity Constraints on the Antarctic Moho and Its Potential Isostatic Adjustments
Authors: Zhang Chaoyang , von Frese Ralph R. B. , Shum C. K. , Leftwich Timothy E. , Kim Hyung Rae , Golynsky Alexander V. 
Published: November 2020

AbstractWe investigate the impact of combining Gravity Field and Steady‐state Ocean Circulation Explorer (GOCE) satellite gravity anomaly and Bedmap2 terrain relief data to enhance Antarctic seismic Moho estimates over the satellite's coverage region south of latitude 60°S. The study considers improving the seismic surface wave‐inferred AN1 Moho (An et al., 2015a, https://doi.org/10.1002/2014JB011332) from misfits of the gravitational effects of the terrain and the seismic Moho computed at 250 km altitude. The updates also relate the GOCE gravity anomalies that correlate directly and inversely with the terrain's gravity effects as isostatic anomalies of the uncompensated mantle relief. These terrain‐correlated effects infer potential isostatic adjustments of the Moho that may help constrain the crust's stress field, track the Gamburtsev Subglacial Mountains to the Kerguelen mantle hotspot, and further test the putative Wilkes Land impact basin for its crustal attributes. Analysis of the gravity‐updated seismic AN1 Moho (sAMoho) estimates suggests that most are within the seismic errors of several kilometers or less. However, the gravity‐updated estimates that are deeper than the sAMoho estimates tend to characterize anomalously hot upper mantle where the assumed mantle‐to‐crust density contrast may be too low. These Moho difference estimates discern elevated heat flow for the Maud Rise, Kerguelen Plateau, Pacific‐Antarctic Ridge, and most of western Antarctica extending from the Pacific‐Antarctic Ridge along the Transantarctic Mountains and across the Ross Sea through Marie Byrd Land and the western margin of the Antarctic Peninsula Microplate to the Scotia Ridge. The methodology of this study also is effective in updating any Moho model for improved gravity and terrain data.
Total Aquatic Carbon Emissions Across the Boreal Biome of Québec Driven by Watershed Slope
Authors: Casas‐Ruiz Joan P. , Hutchins Ryan H. S. , del Giorgio Paul A. 
Published: November 2020

AbstractInland waters emit large amounts of CO2 and CH4 to the atmosphere, partially offsetting the sequestration of carbon in terrestrial ecosystems. However, the incorporation of inland waters into landscape carbon budgets remains challenging, hampered by a lack of studies that consider both carbon gases and the variety of aquatic systems (streams, rivers, and lakes). Here we develop a whole‐network assessment of total aquatic carbon emissions for a set of large watersheds in boreal Québec, Canada. Expressed per unit watershed area, our estimates of total (CO2 + CH4) aquatic carbon emissions range between 11 and 38 g C m−2 yr−1 and cannot be predicted from the size of the watershed or the total surface area of aquatic systems. Rather, we show that total aquatic emissions vary across the boreal landscape of Québec as a function of the average slope of the watershed, which indirectly accounts for the configuration of aquatic networks, the physical forcing that influences gas exchange in fluvial systems, and the potential amount of soil carbon reaching aquatic systems. Total aquatic carbon emissions in boreal Québec are of the same range and magnitude of variation than other components of the boreal carbon budget and could offset terrestrial net ecosystem productivity by as much as 38%.
Advances in transcriptome analysis of human brain aging
Authors: Ham Seokjin , Lee Seung-Jae V. 
Published: November 2020

AbstractAging is associated with gradual deterioration of physiological and biochemical functions, including cognitive decline. Transcriptome profiling of brain samples from individuals of varying ages has identified the whole-transcriptome changes that underlie age-associated cognitive declines. In this review, we discuss transcriptome-based research on human brain aging performed by using microarray and RNA sequencing analyses. Overall, decreased synaptic function and increased immune function are prevalent in most regions of the aged brain. Age-associated gene expression changes are also cell dependent and region dependent and are affected by genotype. In addition, the transcriptome changes that occur during brain aging include different splicing events, intersample heterogeneity, and altered levels of various types of noncoding RNAs. Establishing transcriptome-based hallmarks of human brain aging will improve the understanding of cognitive aging and neurodegenerative diseases and eventually lead to interventions that delay or prevent brain aging.
Stone–Wales defects in hexagonal boron nitride as ultraviolet emitters
Authors: Hamdi Hanen , Thiering Gergő , Bodrog Zoltán , Ivády Viktor , Gali Adam 
Published: November 2020

AbstractMany quantum emitters have been measured close or near the grain boundaries of the two-dimensional hexagonal boron nitride where various Stone–Wales defects appear. We show by means of first principles density functional theory calculations that the pentagon–heptagon Stone–Wales defect is an ultraviolet emitter and its optical properties closely follow the characteristics of a 4.08-eV quantum emitter, often observed in polycrystalline hexagonal boron nitride. We also show that the square–octagon Stone–Wales line defects are optically active in the ultraviolet region with varying gaps depending on their density in hexagonal boron nitride. Our results may introduce a paradigm shift in the identification of fluorescent centres in this material.
Weak acids induce PGE2 production in human oesophageal cells: novel mechanisms underlying GERD symptoms
Authors: Sadatomi Daichi , Kono Toru , Mogami Sachiko , Fujitsuka Naoki 
Published: November 2020

AbstractThe role of weak acids with pH values in the range of 4–7 has been implicated in the symptoms of gastroesophageal reflux disease (GERD). Prostaglandin E2 (PGE2) is associated with heartburn symptom in GERD patients; however, the precise productive mechanisms remain unclear. In this study, we revealed that exposure to weak acids increases PGE2 production with a peak at pH 4–5, slightly in human normal oesophageal cells (Het-1A), and robustly in oesophageal squamous carcinoma cells (KYSE-270). Release of PGE2 from the oesophageal mucosa was augmented by weak acid treatment in rat. Chenodeoxycholic acid (CDCA), a bile acid, upregulated cyclooxygenase-2 (COX-2) expression in Het-1A and KYSE-270 and induced PGE2 production in KYSE-270 cells. Weak acid-induced PGE2 production was significantly inhibited by cytosolic phospholipase A2 (cPLA2), ERK, and transient receptor potential cation channel subfamily V member 4 (TRPV4), a pH-sensing ion channel, inhibitors. Hangeshashinto, a potent inhibitor of COX-2, strongly decreased weak acid- and CDCA-induced PGE2 levels in KYSE-270. These results indicated that weak acids induce PGE2 production via TRPV4/ERK/cPLA2 in oesophageal epithelial cells, suggesting a role in GERD symptoms like heartburn. Interventions targeting pH values up to 5 may be necessary for the treatment of GERD.
EYS is a major gene involved in retinitis pigmentosa in Japan: genetic landscapes revealed by stepwise genetic screening
Authors: Numa Shogo , Oishi Akio , Higasa Koichiro , Oishi Maho , Miyata Manabu , Hasegawa Tomoko , Ikeda Hanako Ohashi , Otsuka Yuki , Matsuda Fumihiko , Tsujikawa Akitaka 
Published: November 2020

AbstractNext-generation sequencing (NGS) has greatly advanced the studies of causative genes and variants of inherited diseases. While it is sometimes challenging to determine the pathogenicity of identified variants in NGS, the American College of Medical Genetics and Genomics established the guidelines to help the interpretation. However, as to the genetic screenings for patients with retinitis pigmentosa (RP) in Japan, none of the previous studies utilized the guidelines. Considering that EYS is the major causative gene of RP in Japan, we conducted stepwise genetic screening of 220 Japanese patients with RP utilizing the guidelines. Step 1–4 comprised the following, in order: Sanger sequencing for two major EYS founder mutations; targeted sequencing of all coding regions of EYS; whole genome sequencing; Sanger sequencing for Alu element insertion in RP1, a recently determined founder mutation for RP. Among the detected variants, 2, 19, 173, and 1 variant(s) were considered pathogenic and 8, 41, 44, and 5 patients were genetically solved in step 1, 2, 3, and 4, respectively. Totally, 44.5% (98/220) of the patients were genetically solved, and 50 (51.0%) were EYS-associated and 5 (5.1%) were Alu element-associated. Among the unsolved 122 patients, 22 had at least one possible pathogenic variant.
Prevalence and status of Lipoprotein (a) among Lebanese school children
Authors: Gannagé-Yared Marie-Hélène , Lahoud Christina , Younes Nada , Chedid Rima , Sleilaty Ghassan 
Published: November 2020

AbstractLipoprotein a (Lp(a) is an independent risk factor for atherosclerotic cardiovascular disease. The prevalence of high Lipoprotein (a) (Lp(a)) in the Lebanese pediatric population is unknown. Our study aims to assess this prevalence and to study the relationship of Lp(a) with the lipid profile, age, body mass index (BMI) and socio-economic status (SES) in Lebanese schoolchildren. A total of 961 children aged 8–18 years (497 boys and 464 girls) were recruited from ten private and public schools in 2013–2014 using a stratified random sample. Schools were selected from the Greater Beirut and Mount Lebanon areas, and were categorized into three subgroups according to the schools’ SES status (high, medium, low). Lp(a) was assayed in 2018 on samples previously frozen at − 80 °C. Abnormal Lp(a) levels (≥ 75 nmol/L) were observed in 14.4% of the overall sample (13.5% for boys,15.3% of girls p = 0.56). The median of Lp(a) was 20(10–50) in the whole sample with no significant gender difference. No significant relationship was found between Lp(a) and age. However, Lp(a) was significantly correlated with BMI in whole sample, as well as in boys and girls (p = 0.02, p = 0.03, p = 0.03, respectively). A significant correlation was found between Lp(a) and non-HDL-C in the whole sample as well as in boys and girls (respectively p < 0.001,p = 0.024 and p = 0.03), but not with triglycerides and HDL-C. In a multivariate linear regression analysis, Lp(a) was only independently associated with BMI and non-HDL-C in boys and girls. Lp(a) was independently associated with BMI and non-HDL-C while no significant relationship was observed with age and sex confirming the strong genetic determination of Lp(a).
Ultraviolet protection of Bacillus thuringiensis through microencapsulation with Pickering emulsion method
Authors: Jalali Elham , Maghsoudi Shahab , Noroozian Ebrahim 
Published: November 2020

AbstractAn encapsulated formulation of Bacillus thuringiensis (Bt) was produced by the Pickering emulsion technique to improve its activity and stability under UV-A radiation. In this technique latex particles, GO nanosheets, olive oil, ethanol, and water were used to encapsulate Bt in colloidosomes. The protective efficacy of this formulation in protecting Bt subsp. Kurstaki against deactivation by UV-A irradiation was measured, so that spore viability and mortality on Ephestia kuehniella (E. kuehniella) Zeller larvae under UV-A radiation are investigated. According to the results of both tests, encapsulated formulation at a concentration of 0.045% has the highest protection of viability. Hence, colloidosome microcapsule formulations successfully provide good protection against UV radiation.
Spontaneous intake of essential oils after a negative postnatal experience has long-term effects on blood transcriptome in chickens
Authors: Foury Aline , Collin Anne , Helbling Jean-Christophe , Leterrier Christine , Moisan Marie-Pierre , Guilloteau Laurence A. 
Published: November 2020

AbstractChicks subjected to early stressful factors could develop long-lasting effects on their performances, welfare and health. Free access to essential oils (EO) in poultry farming could mitigate these effects and potentially reduce use of antimicrobial drugs. This study on chicken analyzed long-lasting effects of post-hatch adverse conditions (Delayed group), and the impact of EO intake on blood physiological parameters and transcriptome. Half of the Control and Delayed groups had free access to EO, while the other half had only water for the first 13 days post-hatching. Blood analyses of metabolites, inflammation and oxidative stress biomarkers, and mRNA expression showed sex differences. Long-lasting effects of postnatal experience and EO intake persisted in blood transcriptome at D34. The early adverse conditions modified 68 genes in males and 83 genes in females. In Delayed males six transcription factors were over-represented (NFE2L2, MEF2A, FOXI1, Foxd3, Sox2 and TEAD1). In females only one factor was over-represented (PLAG1) and four under-represented (NFIL3, Foxd3, ESR2 and TAL1::TCF3). The genes showing modified expression are involved in oxidative stress, growth, bone metabolism and reproduction. Remarkably, spontaneous EO intake restored the expression levels of some genes affected by the postnatal adverse conditions suggesting a mitigating effect of EO intake.
Effects of the technique and drill design used during the osteotomy on the thermal and histological stimulation
Authors: Gehrke Sergio Alexandre , Treichel Tiago Luis Eliers , Aramburú Júnior Jaime , de Aza Piedad N. , Prados-Frutos Juan Carlos 
Published: November 2020

AbstractThe objective of our in vivo study was to compare the effects of the osteotomy on the thermal alterations, the bone healing and count of polymorphonuclear cells, comparing the drill design (cylindrical or conical) using continuous or intermittent movement. Twelve rabbits were used, which were made four osteotomies (n = 2 per tibia) to simulate the surgical drilling sequence for the installation of a dental implant at 8 mm of length and regular diameter. Four groups were proposed: group G1, cylindrical drill with continuous movement; group G2, cylindrical drill with intermittent movement; group G3, conical drill with continuous movement; and, group G4, conical drill with intermittent movement. Thermal mean variation was 6.91 ± 1.4 °C in group 1, 4.30 ± 1.3 °C in group 2, 2.78 ± 0.6 °C in group 3, and 2.77 ± 0.7 °C in group 4. Whereas the mean area of new bone formation was 1.00 ± 0.3 mm2 in group 1, 1.48 ± 0.3 mm2 in group 2, 2.20 ± 0.4 mm2 in group 3, and 2.43 ± 0.4 mm2in group 4. The mean count of polymorphonuclear cells, in the group 1 was 62.4 ± 5.9 cells, group 2 was 50.7 ± 4.2 cells, group 3 was 44.4 ± 3.7 cells, and group 4 was 42.4 ± 3.7 cells. The conical drill sequence produced a significantly smaller increase in temperature during both techniques (continuous and intermittent), more effective new bone formation and a smaller number of polymorphonuclear cells. During the osteotomy for the installation of implants, the professional must take to consider the drill design to perform a less traumatic surgical technique, which can improve and facilitate the healing of peri-implant tissues.
Menopausal factors and risk of seropositive rheumatoid arthritis in postmenopausal women: a nationwide cohort study of 1.36 million women
Authors: Eun Yeonghee , Jeon Keun Hye , Han Kyungdo , Kim Dahye , Kim Hyungjin , Lee Jaejoon , Lee Dong-Yun , Yoo Jung Eun , Shin Dong Wook 
Published: November 2020

AbstractIn previous literature regarding development of rheumatoid arthritis (RA), female reproductive factors have been described as protective factors, risk factors, or irrelevant, leading to inconsistent results. The aim of this study was to investigate the effect of female reproductive factors on the incidence of seropositive RA. A large population-based retrospective cohort of the National Health Insurance Service data in South Korea was used. Postmenopausal women who participated in both cardiovascular and breast cancer screening in 2009 were included and followed until date of seropositive RA diagnosis, death, or December 31, 2018. Multivariable-adjusted Cox proportional hazards model was used to assess the association between reproductive factors and incident seropositive RA. Of 1,357,736 postmenopausal women, 6056 women were diagnosed with seropositive RA, and the incidence rate was 54.16 cases/100,000 person-years. Reproductive factors other than hormone replacement therapy (HRT) were not significantly associated with seropositive RA incidence. Postmenopausal women who used HRT ≥ 5 years were associated with a higher aHR of incident seropositive RA than never-users (aHR 1.25; 95% CI 1.09–1.44). Alcohol consumption less than 30 g per day (aHR 0.80; 95% CI 0.74–0.87), regular physical activity (aHR 0.90; 95% CI 0.84–0.97), diabetes mellitus (aHR 0.85; 95% CI 0.78–0.93), and cancer (aHR 0.77; 95% CI 0.64–0.92) were associated with lower risk of seropositive RA. Most female reproductive factors did not significantly affect the development of seropositive RA in postmenopausal women. Only HRT is associated with a small but significant increase in risk of seropositive RA.
Information theory and dimensionality of space
Authors: Kak Subhash 
Published: November 2020

AbstractWe present an information-theoretic approach to the optimal representation of the intrinsic dimensionality of data and show it is a noninteger. Since optimality is accepted as a physical principle, this provides a theoretical explanation for why noninteger dimensions are useful in many branches of physics, where they have been introduced based on experimental considerations. Noninteger dimensions correlate with lesser density as in the Hausdorff dimension and this can have measurable effects. We use the lower density of noninteger dimension to resolve the problem of two different values of the Hubble constant obtained using different methods.
A pleurocidin analogue with greater conformational flexibility, enhanced antimicrobial potency and in vivo therapeutic efficacy
Authors: Manzo Giorgia , Hind Charlotte K. , Ferguson Philip M. , Amison Richard T. , Hodgson-Casson Alice C. , Ciazynska Katarzyna A. , Weller Bethany J. , Clarke Maria , Lam Carolyn , Man Rico C. H. , Shaughnessy Blaze G. O’ , Clifford Melanie , Bui Tam T. , Drake Alex F. , Atkinson R. Andrew , Lam Jenny K. W. , Pitchford Simon C. , Page Clive P. , Phoenix David A. , Lorenz Christian D. , Sutton J. Mark , Mason A. James 
Published: November 2020

AbstractAntimicrobial peptides (AMPs) are a potential alternative to classical antibiotics that are yet to achieve a therapeutic breakthrough for treatment of systemic infections. The antibacterial potency of pleurocidin, an AMP from Winter Flounder, is linked to its ability to cross bacterial plasma membranes and seek intracellular targets while also causing membrane damage. Here we describe modification strategies that generate pleurocidin analogues with substantially improved, broad spectrum, antibacterial properties, which are effective in murine models of bacterial lung infection. Increasing peptide–lipid intermolecular hydrogen bonding capabilities enhances conformational flexibility, associated with membrane translocation, but also membrane damage and potency, most notably against Gram-positive bacteria. This negates their ability to metabolically adapt to the AMP threat. An analogue comprising d-amino acids was well tolerated at an intravenous dose of 15 mg/kg and similarly effective as vancomycin in reducing EMRSA-15 lung CFU. This highlights the therapeutic potential of systemically delivered, bactericidal AMPs.
Aberrant (pro)renin receptor expression induces genomic instability in pancreatic ductal adenocarcinoma through upregulation of SMARCA5/SNF2H
Authors: Shibayama Yuki , Takahashi Kazuo , Yamaguchi Hisateru , Yasuda Jun , Yamazaki Daisuke , Rahman Asadur , Fujimori Takayuki , Fujisawa Yoshihide , Takai Shinji , Furukawa Toru , Nakagawa Tsutomu , Ohsaki Hiroyuki , Kobara Hideki , Wong Jing Hao , Masaki Tsutomu , Yuzawa Yukio , Kiyomoto Hideyasu , Yachida Shinichi , Fujimoto Akihiro , Nishiyama Akira 
Published: November 2020

Abstract(Pro)renin receptor [(P)RR] has a role in various diseases, such as cardiovascular and renal disorders and cancer. Aberrant (P)RR expression is prevalent in pancreatic ductal adenocarcinoma (PDAC) which is the most common pancreatic cancer. Here we show whether aberrant expression of (P)RR directly leads to genomic instability in human pancreatic ductal epithelial (HPDE) cells. (P)RR-expressing HPDE cells show obvious cellular atypia. Whole genome sequencing reveals that aberrant (P)RR expression induces large numbers of point mutations and structural variations at the genome level. A (P)RR-expressing cell population exhibits tumour-forming ability, showing both atypical nuclei characterised by distinctive nuclear bodies and chromosomal abnormalities. (P)RR overexpression upregulates SWItch/Sucrose Non-Fermentable (SWI/SNF)-related, matrix-associated, actin-dependent regulator of chromatin, subfamily a, member 5 (SMARCA5) through a direct molecular interaction, which results in the failure of several genomic stability pathways. These data reveal that aberrant (P)RR expression contributes to the early carcinogenesis of PDAC.
Enhancing calmodulin binding to cardiac ryanodine receptor completely inhibits pressure-overload induced hypertrophic signaling
Authors: Kohno Michiaki , Kobayashi Shigeki , Yamamoto Takeshi , Yoshitomi Ryosuke , Kajii Toshiro , Fujii Shohei , Nakamura Yoshihide , Kato Takayoshi , Uchinoumi Hitoshi , Oda Tetsuro , Okuda Shinichi , Watanabe Kenji , Mizukami Yoichi , Yano Masafumi 
Published: November 2020

AbstractCardiac hypertrophy is a well-known major risk factor for poor prognosis in patients with cardiovascular diseases. Dysregulation of intracellular Ca2+ is involved in the pathogenesis of cardiac hypertrophy. However, the precise mechanism underlying cardiac hypertrophy remains elusive. Here, we investigate whether pressure-overload induced hypertrophy can be induced by destabilization of cardiac ryanodine receptor (RyR2) through calmodulin (CaM) dissociation and subsequent Ca2+ leakage, and whether it can be genetically rescued by enhancing the binding affinity of CaM to RyR2. In the very initial phase of pressure-overload induced cardiac hypertrophy, when cardiac contractile function is preserved, reactive oxygen species (ROS)-mediated RyR2 destabilization already occurs in association with relaxation dysfunction. Further, stabilizing RyR2 by enhancing the binding affinity of CaM to RyR2 completely inhibits hypertrophic signaling and improves survival. Our study uncovers a critical missing link between RyR2 destabilization and cardiac hypertrophy.