bluuebunny/crossref_metadata_2025_split huggingface dataset:
Is all-inside with suspensory cortical button fixation a superior technique for anterior cruciate ligament reconstruction surgery? A systematic review and meta-analysis
Authors: Fu Chun Wei , Chen Wei-Cheng , Lu Yung-Chang 
Published: May 2020

Abstract
Background To compare the clinical results of all-inside anterior cruciate ligament reconstruction (ACLR) using suspensory cortical button fixation and full tibial tunnel drillingMethods Systematic searches were conducted of published literature up to November 2019 on PubMed, Embase, and Cochrane for studies comparing all-inside ACLR using suspensory cortical button fixation and full tibial tunnel ACLR. Two reviewers independently determined eligibility, extracted the outcome data, and assessed the risk of bias of the eligible studies. The clinical outcome and graft reruptures were pooled by using random effects with mean differences and risk ratios for continuous and dichotomous variables, respectively.Result A total of nine studies (five randomized controlled trials and four comparative studies) involving 613 patients were included in the meta-analysis. The postoperative functional outcome, knee laxity measured with arthrometer, and graft reruptures were comparable between patients with all-inside ACLR using suspensory cortical button fixation and full tibial tunnel ACLR. However, a significantly greater thickness of autologous tendon was used and less change in drilling tunnel diameter was noted in patients with suspensory cortical button graft fixation.Conclusions All-inside ACLR with suspensory cortical button fixation was not clinically superior to full tibial tunnel ACLR with interference screw fixation in functional outcomes, knee laxity measured with arthrometer, or rerupture rate. However, the advantage of using suspensory cortical button fixation was that a thicker graft could be used for reconstruction, and brought less tibia tunnel widening compared with bioabsorbable interference screw fixation.
CFTR improves myocardial ischemic/reperfusion injury through activating FUNDC1-mediated mitophagy and regulating ATP and mitochondrial functions
Authors: Zhang Yaping , Ding Nan , Yi Hanlu , Zhao Yudong , Yan Daole , Shen Lei , Li Zhiqiang , Zhu Yaobin 
Published: May 2020

Abstract
Background

To investigate the potential role of CFTR in myocardial ischemic/reperfusion (I/R) injury and its relationship with mitophagy.
Methods

Wild type (WT) and age matched CFTR−/− male mice were used to establish the myocardial I/R model. CFTR activator forskolin (FSK) was used to activate CFTR in mice. Hypoxia/reoxygenation (H/R) treatment was used for in vitro model in WT or CFTR−/− cardiomyocytes. The autophagy inhibitor 3-MA and activator rapamycin was used for inhibition or activation of autophagy, respectively. The mitochondrial membrane potential (MMP) and ATP concentration were detected. Immunofluorescence was performed for measurement of mitochondria. Oxidative factors reactive oxygen species (ROS), superoxide dismutase (SOD), malondiadehycle (MDA) and glutathione peroxidase (GSH-PX) were detected. The expression of CFTR, MMP-9, TNF-α, IL-8, LC3 II/I, beclin1, caspase-3, caspase-8, caspase-9, bax, bcl-2, p-62 and FUNDC1 was determined using western blotting or PCR.
Results

Knockdown of CFTR significantly increased the infraction volume and decreased the expression of autophagy related proteins beclin1 and LC3II/I in mice. In H/R cardiomyocytes, deficiency of CFTR by induced dysfunction of mitochondrial, decrease of ATP concentration and enhanced oxidative stress, as well as inhibited mitophagy and increased cell apoptosis related protein levels. When treated with 3-MA, the effects of overexpression of CFTR was remarkably reversed, while treatment of rapamycin significantly reversed the effects of inhibiting CFTR on both mitophagy, oxidative stress and cell apoptosis related proteins. The inhibition of FUNDC1 also reversed the above effects of overexpressing CFTR.
Conclusion

Inhibition of CFTR could promote myocardial I/R injury by suppressing FUNDC1-mediated mitophagy and activating of oxidative stress.
Coflex Interspinous Process Dynamic Stabilization for Lumbar Spinal Stenosis: Long-term Follow-up
Authors: Du Mingrui , Wei Fei-Long , Zhu Kai-Long , Zhou Cheng-Pei , Qian Ji-Xian 
Published: May 2020

Abstract
[Objective]To evaluate the long-term efficacy of Coflex interspinous process dynamic stabilization device in the treatment of lumbar spinal stenosis.[Methods]The clinical and imaging data of 73 patients undergoing Coflex interspinous process dynamic stabilization surgery from July 2008 to June 2012 were retrospectively analyzed. Clinical data include: visual analogue scale of pain (VAS), Owestry disability index(ODI) and Japanese Orthopedic Association Scores(JOA) of preoperation, 6 months after surgery, and last follow-up; complications; reoperation rates and incidence of adjacent segment degeneration(ASD). Imaging data including: lumbar range of motion(ROM), intervertebral space height(ISH) and intervertebral foramen height (IFH) of operative and adjacent segment at preoperative, 6 months after operation and the last follow-up.[Results]56 patients were followed up. The follow-up time was 107.6 ± 13.3 months, the operation time was 10.0 ± 3.1 minutes, the intraoperative blood loss was 153.9 ± 80.6 ml, and the hospitalization time was 10.2 ± 3.2 days. The VAS, ODI and JOA scores improved significantly after surgery. At 6 months after surgery and the last follow-up, ROM was significantly lower than that before surgery with statistical significance (P < 0.001). ROM was slightly increased at the last follow-up compared with that 6 months after operation, but the difference was not statistically significant (P > 0.05). The ROM of adjacent segments increased at 6 months and at the last follow-up compared with that before surgery, but the difference was not statistically significant (P > 0.05). At 6 months after surgery, ISH and IFH of implanted segment was significantly higher than that before surgery, and the difference was statistically significant (P < 0.05). At the last follow-up, there was a decrease in ISH and IFH, with no statistical difference compared with that before the operation. During the follow-up period, a total of 11 patients (19.6%) experienced complications and 6 patients (10.7%) underwent secondary surgery.[Conclusion]Coflex interspinous process dynamic stabilization is effective in the treatment of lumbar spinal stenosis in the long term. The surgical segment retains a small range of motion. The incidence of complications and reoperation is low. However, the ISH and IFH of implanted segment can only be maintained for a short period of time.
Comparing Potential Drug-Drug Interactions in Veterinary Medications Using Two Electronic Databases
Authors: Kijtawornrat Anusak , Boonyarattanasoonthorn Tussapon , Khemawoot Phisit 
Published: May 2020

Abstract
Background: One of the most common global health issues in humans and animals is drug-drug interactions (DDIs). This issue increases the risks of health care in both human and veterinary medicine, as animals live long lives and receive many medicines to treat their illnesses. Currently, many electronic databases are using as a tool for potential DDI prediction, e.g., Micromedex and Drugs.com. The purpose of this study was to compare the ability of Micromedex and Drugs.com to detect potential DDIs in veterinary medicines.Results: There were 140 drugs, mainly used for the treatment of disease in animal hospitals, but the Micromedex and Drugs.com databases unidentified 44 items, therefore only 96 items were used in this study. The analysis detected 1,132 potential DDI pairs from the two databases. Micromedex identified 429 pairs of potential DDIs, while Drugs.com identified 842 pairs of potential DDIs. The same severity of the potential DDIs occurred between the two databases for 139 pairs (12.28%) and a different severity was found for 993 pairs (87.72%).Conclusion: Although Micromedex had a lower sensitivity to find out potential DDIs than Drugs.com, Micromedex provided more informative documentation. Veterinary pharmacists should evaluate potential DDIs from different databases and communicate with both the veterinarian and animal owners to ensure an appropriate drug prescription.
Biomass Energy Business Model on Sustainable Bamboo Forest
Authors: Wahono Johannes Widjaja Krisnamurti , Sumarwan M.Sc. Ujang , Arifin M.Sc. Bustanil , Purnomo M.Comp Herry 
Published: May 2020

Abstract
Background: This study objective to create a business model for the development of renewable energy based on bamboo forest biomass for underdeveloped and remote islands in Indonesia. Electrical energy has a vital and strategic role, to support national development and must be realized reliably, safely, equitably and environmentally friendly. Many problems occur in the management of the national electricity system, including dependence on the supply of fossil-fuel primary energy sources, as well as Indonesia's geographical condition which consists of many islands making it difficult to process the transmission and distribution of electrical energy. Electricity generation from renewable energy sources based on bamboo forest biomass can be a solution to the above problems. The research questions to (1) analyze what factors will influence uncertainty in the development of renewable energy based on bamboo forest biomass; (2) analyze the indicators of the success of the development of renewable energy based on sustainable bamboo forest biomass. (3) designing a business model for the development of renewable energy based on bamboo forest biomass in rural areas that can guarantee sustainable regional growth. Methods: The study uses soft systems methodology techniques in the form of case studies supported by field surveys and literature studies. Qualitative analysis was carried out with a Focus Group Discussion and in-depth interviews with experts aimed at identifying indicators of success in developing sustainable renewable energy. The analysis technique uses two analytical methods, Strategic Assumption Surfacing and Testing (SAST) and Interpretative Structural Modeling (ISM). The location of the study was conducted in three villages in Siberut Island, Mentawai Islands Regency, West Sumatra Conclusions: There were numbers of key elements in designing a business model for the development of renewable energy based on bamboo forest biomass, namely electricity subsidy rates, community forest land status, donor agencies, village community participation, income distribution and cooperative partnership of bamboo farmers. Business model that was designed in this study requires five things, specifically; (1) Clustering, (2) High tariffs, (3) Non-commercial funding, (4) Community Forestry, and (5) Community Empowerment.
Anatomic placement of shoulder prosthesis for complex proximal humerus fractures: A modified method for hemiarthroplasty
Authors: Wei Wanfu , Li Jian'an , Bu Guoyun , Yang Tao 
Published: May 2020

Abstract
BackgroundThe placement of shoulder prosthesis used for reconstruction of a complex proximal humerus fractures is challenging. To achieve the anatomical placement of prosthesis, we introduced a new operative technique to determine the humerus height and humeral head retroversion. Herein, we retrospectively investigated the clinical and radiographic outcomes of our procedure.Method34 patients, treated by shoulder arthroplasty for 4-part or 3–part proximal humeral fracture during the period between June 2016 and December 2018, were enrolled in the study. Eventually 29 patients, 18 women and 11 men with an average age of 68.4 years (range 58–85 years), were followed up successfully. Of which 13 patients treated with classic method were matched to 19 of the 29 new method treated patients by operation time, blood loss, pain, range of motion, Constant-Murley score and radiologic features.ResultsThere was significant difference between the two treatment groups in operation time (p = 0.024), while there was no significant difference in blood loss (p = 0.078). No significant difference was found between the two treatment groups in Visual Analog scale (p = 0.225), Constant-Murley score (p = 0.930), and radiological outcomes (p = 0.504). There was linear regression and correlation between Constant-Murley score and age (p = 0.027), while there was no linear relationship between status of tuberosity and the function of the shoulder joint (p = 0.931).ConclusionWe introduce a new operative technique to determine the humerus height and humeral head retroversion for shoulder arthroplasty operation. Based on the data, there is linear regression and correlation between Constant-Murley score and age, while there is no linear relationship between status of tuberosity and the function of the shoulder joint. Our present analyses reveal that our protocol is personalized, easy, effective and feasible.Trial registration: https://doi.org/10.1186/ISRCTN28175069.
miR-22 suppresses cell proliferation, invasion and migration invasion in human colon cancer cell based on targeting NLRP3
Authors: Cong Jinchun , Gong Jian , Yang Chuanjia , Xia Zhixiu , Zhang Hong 
Published: May 2020

Abstract
Background

To investigate the effect of the cell proliferation, migration and invasion in human colon cancer cells which based on the mechanism of miR-22 targeting NLR family pyrin domain containing3(NLRP3)
Methods

Human colonic epithelial cells (HCoEpiC) and human colon cancer cell lines (HCT116, HCT8, HT29, LS174T, LOVO, SW480) were prepared for the present study. The HCT116 cells were divided into 2 parts with different processes, and each part was randomly divided into different groups (part1: BC, NC1, miR-22, NC2, si-miR and part2: BC, miR-22, NC3, si-NLRP3, miR + NC4, miR + NLRP3). Quantification the expression levels of miR-22, NLPR3 and miR-22 targeting NLRP3 related proteins by means of qRT-PCR and Western blot. The colon cancer cell proliferation, invasion and migration were assessed via CCK8, colony formation and transwell assays. Subsequently, nude xenografts mice model was constructed and mice were divided into 4 groups: Model, miR-22, si-NLRP3 and miR + NLRP3. Through the changes of tumor volume and weight, the expression of Ki67 and the protein levels of NLRP3, IL-1B, MMP-9, MMP-2, E-catherine, N-catherine and Vimentin to verify the results.
Results

The cell proliferation, migration and invasion in miR-22, si-NLRP3 and miR + NLRP3 groups were significantly weaker than BC group. In compared to normal tissues, the growth rate of tumor was evidently slowed down, the volume and quality of tumor were evidently reduced and the proportion of Ki67 positive cells were evidently decreased. Both in tumor tissues and in cancer cells, the expression of IL-1B, NLRP3, MMP-9, MMP-2, N-Catherine and Vimentin were evidently decrease and the levels of E-Catherine were evidently increased than in normal tissues or cells. In compared to si-miR and CYLD groups, treatment with si-miR + si-C could effectively reverse the effect of suppressed the tumor progression.
Conclusion

miR-22 targets NLRP3 to inhibit the cell proliferation, migration and invasion in human colon cancer cell
CCL1, IL-22, and IL-36β Have Higher Expression Levels in Aqueous Humor of Neovascular Age-Related Macular Degeneration Patients in Comparison to Cataract Patients
Authors: Chen Kailin , Xu Wen , Zheng Jiao , Shen Yupeng , Ma Jian , Chen Zhiqing 
Published: May 2020

Abstract
Purpose: To investigate the aqueous humor cytokine expression profile in neovascular age-related macular degeneration (nAMD) patients before and after ranibizumab treatments in comparison to cataract patients. Methods: This prospective study included 20 treatment-naïve nAMD eyes of 20 patients who received three consecutive monthly injections of ranibizumab. Aqueous humor samples were collected before the first (baseline), second (1 month later), and third (2 months later) injections. Controls were 20 age- and gender-matched cataract patients without any other ocular disease. The aqueous concentrations of 28 cytokines were measured using a multiplex bead assay. Central macular thickness (CMT) and maximum retinal thickness (MRT)-3mm were measured by spectral domain optical coherence tomography (SD-OCT). The greatest linear diameter (GLD) was measured by fundus fluorescein angiography (FA). Results: Nine cytokines in aqueous humor, including angiogenin, bone morphogenetic protein-9 (BMP-9), C-C motif chemokine ligand 1 (CCL1), CCL13, interleukin-8 (IL-8), fibroblast growth factor-acidic (FGF-α), IL-22, IL-36β, and vascular endothelial growth factor-C (VEGF-C) were significantly higher in nAMD patients in comparison to cataract patients, whether before or after two consecutive monthly ranibizumab injections. Compared with the nAMD patients’ basal levels, two consecutive monthly ranibizumab injections effectively reduced the aqueous concentration of VEGF-A, improved best corrected visual acuity (BCVA), as well as reduced the values of CMT, MRT-3mm, and GLD. Conclusions: Nine cytokines have higher expression levels in nAMD patients in comparison to cataract patients, whether before or after 2 months of ranibizumab therapy. These cytokines may have correlations with the pathogenesis of nAMD.
Bimatoprost promotes satiety and attenuates body weight in rats fed standard or obesity-promoting diets.
Authors: Spada Clayton , Vu Chau , Raymond Iona , Tong Warren , Chuang Chia-Lin , Walker Christopher , Loomes Kerry , Woodward David F. , Poloso Neil 
Published: May 2020

Abstract
Background

Bimatoprost negatively regulates adipogenesis in vitro and likely participates in a negative feedback loop on anandamide-induced adipogenesis. Here, we investigate the broader metabolic effects of bimatoprost action in vivo in rats under both normal state and obesity-inducing conditions.
Methods

Male Sprague Dawley rats were a fed standard chow (SC) diet in conjunction with dermally applied bimatoprost treatment for a period of 9–10 weeks. Body weight gain, energy expenditure, food intake, and hormones associated with satiety were measured. Gastric emptying was also separately evaluated. In obesity-promoting diet studies, rats were fed a cafeteria diet (CAF) and gross weight, fat accumulation in SQ, visceral fat and liver was evaluated together with standard serum chemistry.
Results

Chronic bimatoprost administration attenuated weight gain in rats fed either standard or obesity-promoting diets over a 9–10 weeks. Bimatoprost increased satiety as measured by decreased food intake, gastric emptying and circulating gut hormone levels. Additionally, SQ and visceral fat mass was distinctly affected by treatment. Bimatoprost increased satiety as measured by decreased food intake, gastric emptying and circulating gut hormone levels.
Conclusions

These findings suggest that bimatoprost (and possibly prostamide F2α) regulates energy homeostasis through actions on dietary intake. These actions likely counteract the metabolic actions of anandamide through the endocannabinoid system potentially revealing a new pathway that could be exploited for therapeutic development.
Qualitative Evaluation of the Autism Behavior Inventory: Use of Cognitive Interviewing to Establish Validity of a Caregiver Report Scale for Autism Spectrum Disorder
Authors: Pandina Gahan , Ness Seth , Trudeau Jeremiah , Stringer Sonja , Knoble Naomi , Lenderking William R. , Bangerter Abigail 
Published: May 2020

Abstract
Purpose:The Autism Behavior Inventory (ABI) is an observer-reported outcome scale measuring core and associated features of autism spectrum disorder (ASD). It was developed in alignment with the Food and Drug Administration’s patient-reported outcome guidance to address the need for instruments to measure change and severity of ASD symptoms. Methods:Cognitive interviewing was used following extensive scale development to determine understanding and content validity of the scale prior to its use in a clinical trial.Interviews used a hybrid of the “think-aloud” and verbal probing approach, to assess ABI’s content validity and participant understanding of the instrument, including: (1) item clarity and relevance; (2) item interpretation; (3) appropriateness ofresponse scales,and (4) clarity of instructions. Audio-recordings of the interviews were collected and transcribed for qualitative data analysis.Results: More than 90% of caregivers of individuals with ASD indicated that they understood all but four items in the 70-item ABI, supported by qualitative analysis of responses. Caregivers were able to select an appropriate response from the options available and reported finding the examples helpful. Based on participant feedback from the first 38 interviews, instructions were simplified, 8 items were removed, and 10 items were reworded. The final revised 62-item scale was presented to an additional 12 caregivers, who reported readily understanding the instructions, response options, and item content.Conclusions:Cognitive interviews with caregivers of a diverse sample of individuals with ASD confirm the content validity and relevance of the ABI to assess core and associated symptoms of ASD.
Risk Factors For Severity Of Thrombocytopenia In Full Term Infants: A Single Center Study
Authors: Saber Amira M , Aziz Shereen P , Almasry Al Zahraa E , Mahmoud Ramadan A 
Published: May 2020

Abstract
Background
Neonatal thrombocytopenia (NT) is a common finding in the neonatal intensive care unit (NICU). The main aim of this study was to assess the prevalence, rick factors and outcomes of severe NT in full term (FT) infants.
Method:
During the study period, all FT infants who met the inclusion criteria for NT on two occasions were included. Maternal data such as maternal age, weight, gestational age, mode of delivery, and history of systemic diseases were recorded. Furthermore, neonatal data such as gender, neonatal weight, causes/duration of admission, types of respiratory support used, blood count and outcomes for neonates admitted to the NICU were recorded.
Results
In total, 55 FT infants with NT met the inclusion criteria. In all, 29 (52.73%) cases had severe NT. The most common cause of NT was neonatal sepsis (20 cases, 37.03%), followed by a postoperative state (5 cases, 9.25%). Moreover, in cases of positive blood cultures, the most commonly isolated organism was Escherichia coli (6 cases, 10.90%), followed by Klebsiella (5 cases, 9.09%). Cases of severe NT, when compared to cases of mild/moderate NT associated with signs of bleeding and pulmonary/intraventricular hemorrhage (IVH) (P = 0.001), needed more platelet transfusions (P = 0.001) and had higher rates of mortality (P = 0.001).
Conclusion
Severe NT occurred in 52.73% of cases. The most common cause of NT was neonatal sepsis, followed by a postoperative state. Furthermore, severe NT, when compared to mild/moderate NT associated with signs of bleeding and pulmonary/IVH, needed more platelet transfusions and had increased mortality.