bluuebunny/crossref_metadata_2025_split huggingface dataset:
Validation of methods for effective orifice area measurement of prosthetic valves by two-dimensional and Doppler echocardiography following transcatheter self-expanding aortic valve implantation
Authors: Xiao Minghu , Wu Yongjian , Wang Jingjin , Song Guangyuan , Wang Jiande , Zhu Zhenhui , Wang Xu , Zhao Zhenyan , Wang Hao 
Published: January 2020

Abstract
Background: The aim was to compare two echocardiographic methods for prosthetic valve effective orifice area (EOA) measurement following transcatheter self-expanding aortic valve implantation. Methods: EOA was calculated according to the continuity equation. Two methods were constructed. In Method #1, the left ventricular outflow tract diameter (LVOTd 1 ) was measured at the entry of the prosthesis (from trailing to leading edge); in Method #2, the LVOTd 2 was measured proximal to the prosthetic valve leaflets (from trailing to leading edge). VTI LVOT was recorded by pulse doppler (PW) from apical windows. The region of the PW sampling should match that of the LVOTd measurement with precise localization. Results: A total of 113 consecutive patients were included in the study. All patients were followed up at one year. The mean transvalvular pressure gradient correlated better with the indexed EOA 1 (EOAI 1 ) (r=-0.701, p<0.0001) than EOAI 2 (r=-0.645, p<0.0001). Intra-observer reliability of EOA 1 and was EOA 2 was excellent (ICCs ranging from: EOA 1 : 0.923 to 0.984, and EOA 2 : 0.898 to 0.979, respectively). Interobserver reliability of EOA 1 and was EOA 2 was good (ICCs ranging from: EOA 1 : 0.742 to 0.948, and EOA 2 : 0.589 to 0.901, respectively). Conclusions: For transcatheter self-expanding valve EOA measurement, LVOTd should be measured in the entry of the prosthesis stent (from trailing to leading edge), and VTI LVOT should be measured when the PW sample is located in the entry of the prosthesis stent.
Risk factors for ellipsoid zone integrity after macula-off rhegmatogenous retinal detachment repair
Authors: Fang Wei , Chen Miao , Zhai Jing , Li Jiuke , Chen Yiqi , Li Haidong , Qian Zhenbin , Wang Mengdi , Jin Xiaohong , Li Yumin 
Published: January 2020

Abstract
Background: ellipsoid zone (EZ) layer plays a vital role in visual performance of human retina, hereby we try to find some risk factors for EZ integrity after primary macula-off rhegmatogenous retinal detachment (RRD) repair. Methods: a retrospective cases study. Patients with macula-off RRD undergoing successful primary retinal repair surgery were reviewed and spectral domain optical coherence tomography images of them were analyzed. Comprehensive preoperative, intraoperative and postoperative clinical factors were screened. Results: A total of 118 patients (118 eyes) were enrolled in this study. The mean age of those patients was 52.16 years old (52.16 ±12.87 years). The follow-up time ranged from 0.1 to 84 months (10.21 ±14.81 months). 54 cases (45.76%) had their EZ fully reconstructed at final visit. The mid-quartile time of EZ reconstruction was 14.0 months (95% CI: 11.3 ~20.0 months). Multivariate proportional haphazard regression test revealed that the independent factors were: silicone oil tamponade (Hazard ratio=0.414, p=0.0400), posterior staphyloma (Hazard ratio=0.141, p=0.0021) and disorganization of retinal inner layer (Hazard ratio=0.167, p=0.0166). Conclusion: After successful retinal reattachment for macula-off retinal detachment, the mid-quartile time of EZ recovery was about 14 months. The independent risk factors for EZ recovery might include silicone oil tamponade, posterior staphyloma and disorganization of retinal inner layer.
Environmental Exposure to Cooking Oil Fume and Fatty Liver Disease: a cross-sectional study.
Authors: Zhang Shun , Lin Jie , Ni Saili , Shi Qili , Xiong Zi , Kang Junfei , Sun Xiaohua , Gao Yumeng , Zhang Huijing , Liu Shanyi , Cai Ting 
Published: January 2020

Abstract
Background The effect of cooking oil fume on development of fatty liver disease is limited. The present study aims to investigate the association between exposure to cooking oil fume and the risk of fatty liver disease. Method A total of 55959 participants aged between 40 and 75 years old participated in a community-based survey in Ningbo, China. Multiple logistic regression analyses were conducted to investigate the association between cooking oil fume exposure and fatty liver risk. Furthermore, ordered logistic regression was conducted to investigate the association between cooking oil fume exposure and the severity of fatty liver disease. Results Cooking oil fume exposure were significantly associated with fatty liver disease after adjusting for confounding factors compared with participants in the none oil fume exposure group. Moreover, interaction analyses indicated that females with heavy oil fume exposure had the highest odds ratios of fatty liver disease and severer disease extent. In the stratified analysis, compared to participants in the smokeless group, males and females in light, moderate and heavy cooking oil fume exposure groups all had significantly higher risk of fatty liver disease and severer disease extent, while participants with heavier cooking oil fume exposure tended to have higher risk of fatty liver disease and severer disease extent. Conclusion Our findings indicated that exposure to cooking oil fume potentiated the risk of fatty liver disease, and the associations might be dose-responsive. Furthermore, heavy oil fume exposure and female sex might have a synergistic effect on fatty liver disease.
Successful Removal of Cranial Migrated Intravitreal Perfluorocarbon through A Lumbar Puncture
Authors: Chen Lin , Zhang Zhongheng , Wang Qing , Ni Hongying 
Published: January 2020

Abstract
Background: Perfluorocarbon migrating into subarachnoid was very rare let alone its further removing from central nervous system. Case presentation: We report a case of migration of intravitreal perfluorocarbon into cranial space and further removed through a lumbar puncture treatment. The patient showed a sudden onset of hypoxemia and hypotension during an ocular surgery, which was highly suspected as neurogenic pulmonary edema caused by the migrated perfluorocarbon. The patient's symptoms improved after extracorporeal membrane oxygenation (ECMO). The perfluorocarbon gradually descended through subarachnoid space to lumbar cisterna and finally drained by a lumbar puncture. Conclusions: Perfluorocarbon could enter the cranial space during ocular surgery and threaten the life of patient. Further migrating through subarachnoid space provide the opportunity to remove it.
Evaluation of the Effectiveness of Continuous Venovenous Hemodiafiltration Appli̇ed With Oxiris and AN69 Membranes in Patients With Septic Shock-Related Acute Kidney Injury
Authors: Kaçar Cem Kıvılcım , Uzundere Osman , Yüksel Enver , Kandemir Deniz , Bıçak Esra Akiz , Yektaş Abdulkadir 
Published: January 2020

Abstract
Background and objectives: AN69 and Oxiris are filters used in continuous renal replacement therapy. In this study, we aimed to research the effects of these filters on blood cell counts, blood biochemistry, inflammation indicators, clinical status and mortality of patients diagnosed with septic shock-related acute kidney injury. Method: Between March 2019 and October 2019, 42 adult patients (Group 1: Oxiris (n = 21) or Group 2: AN69 (n=21)) with septic shock-related acute kidney injury and received continuous venovenous hemodiafiltration (CVVHDF) in the intensive care unit were included in the study and their results were prospectively observed and compared. The data at the begining of CVVHDF (pre-CVVHDF) and 24 hours after the onset of CVVHDF (post-CVVHDF) were recorded.Results: In the comparison of the pre- and post-CVVHDF values in Group 1, there was a statistically significant decrease detected in the procalcitonin (p = 0.04) and noradrenaline infusion rate (p = 0.02) levels. In terms of the other data there was no statistically significant difference between pre- and post-CVVHDF values in Group 1. In the comparison of the pre- and post-CVVHDF values in Group 2, there was a statistically significant decrease detected in the urea (p = 0.04), platelet count (p = 0.02) and procalcitonin (p = 0.002) levels. There was no statistically significant difference between pre- and post-CVVHDF values in terms of the other data in Group 2. There was no statistically significant difference between the groups in terms of mortality. Conclusions: CVVHDF with Oxiris filter causes a statistically significant decrease in noradrenaline infusion rate. Therefore, we think that the use of CVVHDF with Oxiris filter applied for septic shock-related acute kidney injury will save us time and increase the improvement in the treatment.
Rituximab treatment for steroid dependent or steroid resistant nephrotic syndrome in adult patients
Authors: Huang Jing , Wang Ping , Cui Zhao , Zhang Yimiao , Wang Fang , Meng Liqiang , Cheng Xuyang , Liu Gang , Zhou Fude , Zhao Minghui 
Published: January 2020

Abstract
Background: Minimal change disease (MCD) and focal segmental glomerular sclerosis (FSGS) are major causes of nephrotic syndrome (NS). The patients of steroid-dependent (SD) or steroid-resistant (SR) NS are exposed to high doses of steroid, more adverse effects, and worse outcomes. This study applied B cell-oriented rituximab therapy in MCD or FSGS adult patients with SD or SR, to investigate its efficiency and safety. Methods: Eight patients with steroid-dependent and frequent-relapsing (SD/FR) NS and six patients with steroid-resistant (SR) NS were enrolled. B-cell-oriented (<5 cells/mm 3 ) rituximab administration was used with single dose of 375 mg/m 2 adjusted according to eGFR. Results: During the follow-up period of 15.0 (8.8-18.0) months, B-cell depletion was achieved and maintained in all the 14 patients. Four, two, seven, one patients received two, three, four, five infusions of rituximab respectively. No adverse event was observed. All the eight SD/FR patients maintained complete remission without relapse. Six of them stopped steroid in 10 (2.3-12.3) months and four of them further stopped immunosuppressants. All of them maintained stable kidney function (eGFR 107.4 ± 27.4 vs. 111.0 ± 31.5 mL/min/1.73m 2 , P=0.600). All the six SR patients showed no response and presented with severe nephrotic syndrome. Five of them presented with kidney function deterioration (eGFR 49.6 ± 35.7 vs. 15.9 ± 11.5 ml/min/1.73m 2 , P=0.047) and three of them went into ESRD. Conclusion: B cell depletion-oriented regimen of rituximab was effective and safe for MCD or FSGS adult patients with SD/FR nephrotic syndrome, which could reduce drug doses and adverse events. This regimen showed no therapeutic effect for SR patients.
Aspirin attenuates L-DOPA-induced dyskinesia in hemi-parkinsonian rats
Authors: Zhu Guo-Fu , Zheng Chang-Qing , Li Dai-Di , Zhou Yan-Zhen , Li Jing-Jie , Wang Guo-Qing , Zhang Feng 
Published: January 2020

Abstract
Background: At present, L-DOPA remained the gold standard therapy for motor symptoms of Parkinson’s disease (PD) patients. However, prolonged administration of L-DOPA led to the development of dyskinesia. Aspirin, a non-steroidal anti-inflammatory drug (NSAID), had been widely used to relieve pain and inflammation. Recent studies indicated aspirin produced neuroprotection against dopamine (DA) neuronal loss in animal model. This study aimed to explore the effects of aspirin pre-treatment and co-treatment with L-DOPA on DA neurotoxicity and L-DOPA-induced dyskinesia (LID) as well.Methods: Rats received a single 6-hydroxydopamine (6-OHDA) injection into substantia nigra to induce DA neuronal loss. For aspirin pre-treatment before L-DOPA studies: One day after 6-OHDA stimulation, rats were daily administrated with aspirin for 3 weeks followed by daily L-DOPA treatment along with aspirin for additional 3 weeks. For aspirin co-treatment with L-DOPA studies: Three weeks after 6-OHDA administration, rats were daily treated by L-DOPA together with aspirin for another 3 weeks. DA neurotoxicity was analyzed via rat PD-like behavior test and DA neuronal counting. The movement disorders of dyskinesia triggered by L-DOPA were determined by the abnormal involuntary movements (AIM) scores analysis.Results: we demonstrated both aspirin pre-treatment and co-treatment exerted anti-LID effects during L-DOPA treatment on 6-OHDA-lesioned rats. In addition, aspirin not only ameliorated DA neuronal damage, but also reduced the development of dyskinesia without affecting L-DOPA efficacy. Furtherly, inhibition of glial cells activation and the subsequent neuroinflammatory response might be involved in aspirin-attenuated dyskinesia.Conclusion: the present study suggested aspirin could have beneficial potential to attenuate LID in PD.
A signature related to lung adenocarcinoma prognosis: A study based on TCGA database
Authors: Wang Jia , Zhang Xiaolu , Zhang Xiaoming , Yao Yan , Ma Xiaoran , Xu Xiaowei , Zhuang Jing , Liu Lijuan , Sun Peng , Wu Jibiao , Sun Changgang 
Published: January 2020

Abstract
Background: The intrinsic molecular subtypes of lung adenocarcinoma (LUAD) impact clinical treatment decision-making, but the molecular mechanisms are still unclear. Therefore, we aimed to identify sensitive biomarkers to evaluate LUAD patient prognosis. Methods: Differentially expressed RNAs from LUAD patients were obtained from The Cancer Genome Atlas (TCGA) database and they were used to construct a competitive endogenous RNA (ceRNA) network. Based on the examination of clinical data, long noncoding RNAs (lncRNAs) and mRNAs in the network were selected by univariate and multivariate Cox regression analysis. Finally, functional enrichment analysis was used to reveal prognostic signatures based on the classification into high and low-risk groups, survival analysis, and an independence test. Results: The ceRNA network consisted of 21 mRNAs, 53 lncRNAs, and 8 miRNAs that were selected from the differentially expressed RNAs identified. Next, based on univariate and multivariate Cox regression analysis, a prognostic signature, including two mRNAs (HOXA10 and CBX2) and four lncRNAs (LINC00460, LINC00330, DGCR5, and C14orf132) was constructed. Eventually, survival analysis showed that significant differences in survival rates between high and low-risk groups and the area under the curve (AUC) for three‐year survival was 0.714. Compared with clinical risk factors, including age, pathological stage, and TNM stage, our risk score had a higher prognostic value. Conclusion: By screening from a ceRNA network, we constructed a signature, including two mRNAs (HOXA10 and CBX2) and four lncRNAs (LINC00460, LINC00330, DGCR5, and C14orf132), that can be utilized as a prognostic biomarker in LUAD. This signature may provide options for clinical treatment.
Patterns of Infections and Antimicrobial Drugs’ Prescribing among Pregnant Women in Saudi Arabia
Authors: Baraka Mohamed A , AlLehaibi Lina Hussain , AlSuwaidan Hind Nasser , Alsulaiman Duaa , Islam Mohamed Ashraful , Alotaibi Badriyah S. , Alboghdadly Amany , Homoud Ali H. , Al-Ghamdi Fuad H. , Ghamdi Mastour S Al 
Published: January 2020

Abstract
Background Antimicrobial agents are considered among the most commonly prescribed drugs in pregnancy given the increased susceptibility of infections during pregnancy. Antimicrobials can contribute to different maternal complications. Therefore, it is important to study their prescribing and utilization pattern. Such type of data regarding this issue is scarce in Saudi Arabia and this encouraged us to conduct this research. The aim of this study is to generate data about the most commonly prescribed antimicrobial agents during pregnancy, as well as their indicators and safety. Methods This is a retrospective study focusing on pregnant women with a known antimicrobial consumption at Johns Hopkins Aramco Healthcare (JHAH). The sample size was 344 pregnant women with 688 antimicrobial prescriptions. Data was collected about prevalence of infections and antimicrobial prescriptions in addition to drug safety during pregnancy using FDA risk categorization system. Results The results showed that Urinary Tract Infections (UTIs) were the most reported (59%) infectious diseases. Around 48% of pregnant women received antimicrobial medications at some point during their pregnancy. The top two antimicrobial agents based on prescription frequency were B-lactams (44.6%) and azole anti-fungals (30%). The prescribed drugs in the study were found to be from B, C and D classes according to the FDA risk classification system. Conclusion Overall antimicrobial prescribing practices were appropriate. The study revealed a high prevalence of antimicrobials prescribing during pregnancy that might pose risks to mothers and their fetuses. Future multi center studies are warranted to evaluate the rational prescribing of antimicrobial medications during pregnancy.
Community-acquired Klebsiella pneumoniae central nervous infection with acute suppurative otitis and bacteremia: a case report in mainland China
Authors: Sun Ruixue , Zhang Hui , Xu Yingchun , Zhu Huadong , Yu Xuezhong , Xu Jun 
Published: January 2020

Abstract
Community-acquiredKlebsiella pneumoniae (K. pneumoniae) central nervous system (CNS) infection combined with bacteremia was rarely identified world. We received a 55-year-old womanwho was under hormone therapy because of Sjogren's syndrome.The onset of this case was acute suppurative otitis,then she developed headache and unconscious, the cerebrospinal fluid (CSF) and imaging examinations were compatible with K. pneumoniae meningitis and suspicious brain abscesses, and K. pneumoniae bacteremia was also combined. Even with adequate antibiotic and supportive therapy, the patient’ relatives refused further assessment of intracranial lesions and took the patient back home, finally the patient died of no spontaneous breathing.Hormone therapy might be the risk factor of community-acquiredK. pneumoniaeinfection. Appropriate antibiotic application and infection focus drainage should be tried for treating this condition. The reason of patient’s death might be the CNS infection combined with K. pneumoniaebacteremia and the absence of intracranial lesion elimination. This case is reported in order to help us know this rare disease and promote a further progress on its pathogenesis and treatment.
Identification of hub genes and a prognostic model associated with ovarian cancer
Authors: Liu Jinhui , jiang pinping , Meng Huangyang , Li Siyue , Nie Sipei , Rui Sun , Jing Yang , Cheng Wenjun 
Published: January 2020

Abstract
Background Ovarian cancer (OC) is one of the most lethal gynecological cancers worldwide. The pathogenesis of the disease and outcomes prediction of ovarian cancer patients remain largely unclear. This study aimed to explore the key genes and biological pathways in ovarian carcinoma development, as well as construct a prognostic model to predict patients’ overall survival. Results We identified 164 up-regulated and 80 down-regulated differentially-expressed genes (DEGs) associated with ovarian cancer. GO term enrichment showed DEGs mainly correlated with spindle microtubes. For KEGG pathways, cell cycle was mostly enriched for the DEGs. The PPI network yielded 238 nodes and 1284 edges. Top three modules and 10 hub genes were further filtered and analyzed. Three candidiate drugs targeting for therapy were also selected. Thirteen OS-related genes were selected and an eight-mRNA model was present to stratify patients into high- and low-risk groups with significantly different survival. Conclusions The identified DEGs and biological pathways may provide new perspective on the pathogenesis and treatments of OC. The identified 8-mRNA signature has significant clinical implication for outcome prediction and tailored therapy guidance for OC patients.
The gut microbiome is more stable in males than in females during the development of colorectal cancer
Authors: Liao Haijun , Li Chaonan , Ai Yingwei , Kou Yongping 
Published: January 2020

Abstract
Background Gut microbial shifts have great potential to predict the risk of colorectal cancer, but how gut microbes respond to the development of colorectal cancer in males and females at the community scale is unknown. To address this question, we reanalyzed the dataset from a published project and grouped these data depending on sex into three groups, healthy, colorectal adenoma and carcinoma individuals, and community assembly and network patterns of gut microbes were evaluated by null model and co-occurrence network-based methods. Results The study showed that distinct changes in microbial α -diversities and community composition were observed between the male and female gut. Specifically, the microbial α -diversities did not significantly change in the male gut ( P > 0.05) but were lowered in the female gut ( P < 0.05), suggesting that permanent species loss might occur in the female gut during the development of colorectal cancer. These distinct changes in microbial α -diversities and community composition between the male and female gut might result from host immunity mechanisms and different microbial community assemblies in males and females. Indeed, we found that the microbial community tended to be primarily stochastic in males, whereas it became increasingly deterministic in females with the development of colorectal cancer. Moreover, microbial co-occurrence associations tended to be more complicated when communities were primarily driven by stochastic processes in males; rare species were enriched in the co-occurrence network of the male gut, whereas key species loss was observed in the co-occurrence network of the female gut. Conclusions Our findings indicate that microbial communities in the male gut are more stable than those in the female gut and that microbial community assembly in the gut is sex-dependent with the development of colorectal cancer. Our study suggests that sexual dimorphism needs to be taken into account to better predict the risk of colorectal cancer based on microbial shifts.