bluuebunny/crossref_metadata_2025_split huggingface dataset:
Small Intestinal Mucosal Injury not Associated with Acute Gastrointestinal bleeding Induced by Aspirin: a retrospective observational study
Authors: Gui Qian , Liang Xiao , Ge Zhi Zheng , Xiong Hua , Zhu Qi , Lu Hong 
Published: June 2020

Abstract
Background

To investigate the characteristics of small intestinal injuries and its association with upper gastrointestinal ulcers and Helicobacter pylori infection in aspirin induced acute gastrointestinal bleeding patients.
Methods

Esophagogastroduodenoscopy and capsule endoscopy were used to examine the gastrointestinal injuries. Serum antibody was used to test the history of Helicobacter pylori infection. Clinical history, underlying diseases, the duration of aspirin use, and proton pump inhibitors use were recorded.
Results

A total of 72 patients were involved with 58 cases (80.6%) of active upper gastrointestinal peptic ulcers and 9 cases (12.5%) with small intestinal injuries (erosive/ulcerative lesions). Fifty percent of patients (36 cases) was reported Helicobacter pylori infection.
Conclusions

It was upper gastrointestinal ulcers that accounting for most of aspirin-induced acute gastrointestinal bleeding instead of the intestinal mucosal injuries.
Sex difference in the incidence of cardia and non-cardia gastric cancer in the United States, 1992-2014
Authors: Yao Qiang , Qi Xiaona , Xie Shao-Hua 
Published: June 2020

Abstract
Background Gastric cancer is more common in men than in women, but underlying reasons have not been completely understood.

Methods Using data from 13 cancer registries in the Surveillance, Epidemiology, and End Results Program, we analyzed the age-specific sex difference in the incidence of gastric cancer in the United States during 1992-2014. We assessed the temporal trends in the sex differences in the incidence of gastric cancer during the study period.

Results The male-to-female incidence ratio of cardia cancer increased with age until peaking at ages 55-69 years and decreased thereafter, while the ratio for non-cardia gastric cancer increased with age before ages <60 years and remained stable onwards. The age-specific patterns in the sex difference of gastric cancer incidence varied between intestinal and diffuse histological types. The sex difference in the incidence of cardia cancer remained relatively stable except for that the absolute difference between the sexes in whites decreased on average by 0.8% per year from 1992 to 2014. The absolute incidence difference between the sexes in non-cardia gastric cancer decreased over time in whites, blacks, and Asian and Pacific islanders by approximately 4% per year. The male-to-female incidence ratio of non-cardia gastric cancer decreased over time in whites and blacks, but remained relatively stable in Asian and Pacific islanders.

Conclusions Both extrinsic and intrinsic factors may have contributed to the sex difference in gastric cancer. Sex hormones may play a role in the development of cardia cancer and intestinal type of gastric cancer.
Assessment of Novice Learners in a Novel High-fidelity Simulation-Based Extracorporeal Membrane Oxygenation Education Program
Authors: TONG Li , HU Wen-jie , ZHU Yan-ping , CAO Lu , LI Ying-hui , ZHANG Ning , CAI Chang-jie , KUANG Ming 
Published: June 2020

Abstract
Background: This study aimed to design a suitable integrated extracorporeal membrane oxygenation (ECMO) curriculum and assessed the impact of this a high-fidelity simulation-based education module on novice learners.
Methods: An ECMO training curriculum was developed by well-trained and experienced ECMO experts that incorporated simulation modules to train multidisciplinary health care professionals. Pre- and post-participation questionnaires were used to determine the effects on the knowledge, ability, and confidence level of the participants. A five-point Likert scale was used to assess the participants regarding the ECMO practical test.
Results: The study enrolled 37 participants (10 doctors, 27nurses). 67.6% (25/37) of trainees didn’t have ECMO experience. All two questionnaires were completed by 37 participants. A five-point Likert scale on practical test was finished by 4 ECMO experts. All participants passed the written and practical tests. All the responding participants thought the curriculum was useful in improving their perception of their overall knowledge and their ability to perform the required critical performance criteria on simulated ECMO. 91.9% (34/37) trainees thought that they invested more in active learning during the whole course; 60% (22/37) of them achieved a good level of technical evaluation. On the other hand, 67.6% (25/37) of the trainees have shown a good self-confidence in the priming course; however, 43.2% (16/27) trainees still needed to strengthen their overall competence in ECMO management.
Conclusions: The integration of traditional teaching and high-fidelity simulation teaching can effectively improve the ECMO monitoring level and management skills of novice learners, but whether this training mode can be successfully transferred to the clinical field needs further research and confirmation.
Expression, Purification and Characterization of Recombinant Human Coagulation Factor XIIIa in Pichia Pastoris
Authors: Cheng Linyan , Zhang Ting , Fei Yuchang , Shen Hao , Huang Hui , Chen Jin , Xu Bin , Xu Jian 
Published: June 2020

Background:
Coagulation factor XIIIa(FXIIIa) plays a critical role in the final stage of
blood coagulation. It is extremely important in wound healing, tissue repairing and promoting cell
adhesion. The deficiency of the coagulation factor can cause hemorrhage and slow wound healing.

Objective:
In this study, recombinant pPICZαC-FXIIIa was expressed in Pichia pastoris, purified
as well as its biological activity was determined.

Methods:
The FXIIIa fragment obtained from the human placenta was inserted into pPICZαC to
obtain pPICZαC-FXIIIa, which was transformed into X33 after linearization, and FXIIIa inserted
into Pichia pastoris X33 was screened for methanol induction. The expressed product was identified
by western blotting, then the supernatant was purified by affinity chromatography, and the purified
product was determined by plasma coagulation experiment.

Results:
Polymerase Chain Reaction(PCR) showed that the FXIIIa fragment of 2250 bp was inserted
successfully into pPICZαC. The expression and purification products of the same molecular
weight as target protein(about 83 kDa) were obtained, which solidified significantly when reacted
with plasma.

Conclusion:
The expression and purification products were successful, with sufficient biological
activity, which can be used as a candidate FXIIIa hemostatic agent in genetic engineering.
A Knock-Down Cell-Based Study for the Functional Analysis of Chloride Intracellular Channel 1 (CLIC1): Integrated Proteomics and Microarray Study
Authors: Yin Shang-Jun , Lee Jae-Rin , Lee Bit-Na , Yang Jun-Mo , Qian Guo-Ying , Park Yong-Doo , Hahn Myong-Joon 
Published: June 2020

Background:
Previously, we detected that chloride intracellular channel 1 (CLIC1) was
involved in the pathogenesis of atopic dermatitis (AD).

Objective:
In this study, we aimed to use high-throughput screening (HTS) approaches to identify
critical factors associated with the function of CLIC1 in knock-down cells.

Methods:
We down-regulated CLIC1 in human A549 cells via siRNA and then conducted serial
HTS studies, including proteomics integrated with a microarray and the implementation of bioinformatics
algorithms.

Results:
Together, these approaches identified several important proteins and genes associated
with the function of CLIC1. These proteins and genes included tumor rejection antigen (gp96) 1,
nucleophosmin, annexin I, keratin 1 and 10, FLNA protein, enolase 1, and metalloprotease 1,
which were found using two-dimensional electrophoresis (2-DE) proteomics. Separately, NTNG1,
SEMA5A, CLEC3A, GRPR, GNGT2, GRM5, GRM7, DNMT3B, CXCR5, CCL11, CD86, IL2,
MNDA, TLR5, IL23R, DPP6, DLGAP1, CAT, GSTA1, GSTA2, GSTA5, CYP2E1, ADH1A, ESR1,
ARRDC3, A1F1, CCL5, CASP8, DNTT, SQSTM1, PCYT1A, and SLCO4C1 were found using
a DNA microarray integrated with PPI mapping.

Conclusion:
CCL11 is thought to be a particularly critical gene among the candidate genes detected
in this study. By integrating the datasets and utilizing the strengths of HTS, we obtained new insights
into the functional role of CLIC1, including the use of CLIC1-associated applications in the
treatment of human diseases such as AD.
Critical Review of Data Analytics Techniques used in the Expanded Program on Immunization (EPI)
Authors: Qazi Sadaf , Usman Muhammad 
Published: June 2020

Background:
Immunization is a significant public health intervention to reduce child
mortality and morbidity. However, its coverage, in spite of free accessibility, is still very low in developing
countries. One of the primary reasons for this low coverage is the lack of analysis and
proper utilization of immunization data at various healthcare facilities.

Purpose:
In this paper, the existing machine learning-based data analytics techniques have been reviewed
critically to highlight the gaps where this high potential data could be exploited in a meaningful
manner.

Results:
It has been revealed from our review that the existing approaches use data analytics techniques
without considering the complete complexity of Expanded Program on Immunization which
includes the maintenance of cold chain systems, proper distribution of vaccine and quality of data
captured at various healthcare facilities. Moreover, in developing countries, there is no centralized
data repository where all data related to immunization is being gathered to perform analytics at various
levels of granularities.

Conclusion:
We believe that the existing non-centralized immunization data with the right set of
machine learning and Artificial Intelligence-based techniques will not only improve the vaccination
coverage but will also help in predicting the future trends and patterns of its coverage in different
geographical locations.
Synthesis and In-silico Identification of New Bioactive 1,3,4-oxadiazole Tagged 2,3-dihydroimidazo[1,2-a]pyridine Derivatives
Authors: Jadhav Bhagwat S. , Purohit Vipul P. , Yamgar Ramesh S. , Kenny Rajesh S. , Mali Suraj N. , Chaudhari Hemchandra K. , Mandewale Mustapha C. 
Published: June 2020

Background:Tuberculosis (TB) continues to be the most threatening cause of death in recent years. There is urgent need of search more potent, less toxic antitubercular agents.Methods:A set of five new 1,3,4-oxadiazolyl-imidazo-1,2-pyridine derivatives (4a-4e) was synthesized and screened invitro for their antibacterial activity against Mycobacterium tuberculosis (H37 RV strain) ATCC No-27294.Results:Compound 4b displayed potent antitubercular activity at MIC 6.25 µg/mL. In-silico molecular docking studies were performed for evaluation of the binding patterns of compounds 4a-4e in the binding site of proteins like, Pantothenate synthatase and enoyl acyl reductase inhibitor. The outcomes of the in- vitro antitubercular studies were in well agreement with the molecular docking studies. These newly synthesized compounds were found to have good ADMET profile. We also explored possible anticancer activity using in-silico methods.Conclusion:These results shows that readily synthesized 1,3,4-oxadiazolyl-imidazo-1,2-pyridine derivatives (4a-4e) are attracting new class of potent anti-TB targets as well as possible anticancer activity that worth additional opportunities for improvements.
Novel Toxin-antitoxin System Xn-mazEF from Xenorhabdus nematophila: Identification, Characterization and Functional Exploration
Authors: Nim Jogendra Singh , Yadav Mohit , Gautam Lalit Kumar , Ghosh Chaitali , Sahi Shakti , Rathore Jitendra Singh 
Published: June 2020

Background:
Xenorhabdus nematophila maintains species-specific mutual interaction
with nematodes of Steinernema genus. Type II Toxin Antitoxin (TA) systems, the mazEF TA system
controls stress and programmed cell death in bacteria.

Objective:
This study elucidates the functional characterization of Xn-mazEF, a mazEF homolog in
X. nematophila by computational and in vitro approaches.

Methods:
3D- structural models for Xn-MazE toxin and Xn-MazF antitoxin were generated, validated
and characterized for protein - RNA interaction analysis. Further biological and cellular functions
of Xn-MazF toxin were also predicted. Molecular dynamics simulations of 50ns for Xn-
MazF toxin complexed with nucleic acid units (DU, RU, RC, and RU) were performed. The MazF
toxin and complete MazEF operon were endogenously expressed and monitored for the killing of
Escherichia coli host cells under arabinose induced tightly regulated system.

Results:
Upon induction, E. coli expressing toxin showed rapid killing within four hours and attained
up to 65% growth inhibition, while the expression of the entire operon did not show significant
killing. The observation suggests that the Xn-mazEF TA system control transcriptional regulation
in X. nematophila and helps to manage stress or cause toxicity leading to programmed death of
cells.

Conclusion:
The study provides insights into structural and functional features of novel toxin, Xn-
MazF and provides an initial inference on control of X. nematophila growth regulated by TA systems.
Anti-tubercular Modelling via QSAR Approach, In Silico Design and Virtual
Docking Screening of Designed Hypothetical Inhibitors Against DNA
gyrase Protein
Authors: Adeniji Shola Elijah 
Published: June 2020

Introduction:
Mycobacterium tuberculosis has instigated a serious challenge toward the effective treatment of
tuberculosis. The reoccurrence of the resistant strains of the disease to accessible drugs/medications has mandate for the
development of more effective anti-tubercular agents with efficient activities. Time expended and costs in discovering and
synthesizing new hypothetical drugs with improved biological activity have been a major challenge toward the treatment
of multi-drug resistance strain M. tuberculosis (TB). Meanwhile, to solve the problem stated, a new approach i.e. QSAR
which establish connection between novel drugs with a better biological against M. tuberculosis is adopted.

Methods:
The
anti-tubercular model established in this study to forecast the biological activities of some anti-tubercular compounds
selected and to design new hypothetical drugs is subjective to the molecular descriptors; MATS7s, SM1_DzZ,
SpMin4_Bhv, TDB3v and RDF70v. Ligand-receptor interactions between quinoline derivatives and the receptor (DNA
gyrase) was carried out using molecular docking technique by employing the PyRx virtual screening software and
discovery studio visualizer software. Furthermore, docking study indicates that compounds 20 of the derivatives with
promising biological activity have the utmost binding energy of -17.79 kcal/mol.

Results:
Meanwhile, the interaction of
the standard drug; isoniazid with the target enzyme was observed with the binding energy -14.6 kcal/mol which was
significantly lesser than the binding energy of the ligand (compound 20).Therefore, compound 20 served as a template
structure to designed compounds with more efficient activities. Among the compounds designed; compounds 20p was
observed with better anti-tubercular activities with more prominent binding affinities of -24.3kcal/mol.

Conclusion:
The
presumption of this research aid the medicinal chemists and pharmacist to design and synthesis a novel drug candidate
against the tuberculosis. Moreover, in-vitro and in-vivo test could be carried out to validate the computational results.
Whole-exome Sequencing of Tumor-only Samples Reveals the Association between Somatic Alterations and Clinical Features in Pancreatic Cancer
Authors: Ran Wenwen , Chen Xiangbin , Wang Bo , Yang Ping , Li Yongxing , Xiao Yujing , Wang Xiaonan , Li Guangqi , Wang Lili , Han Yingmin , Peng Yonggang , Lang Jidong , Liang Yuebin , Xiao Yupei , Lu Qingqing , Lin Huixin , Tian Geng , Yuan Dawei , Deng Chaoyang , Yang Jialiang , Xing Xiaoming 
Published: June 2020

Background:
Identification of genomic markers using NGS (next-generation sequencing)
technology would be valuable for guiding precision medicine treatments for pancreatic cancers.
Traditional somatic mutation methods require both tumor and matched non-tumor samples.
However, only tumor samples are available mostly, especially in retrospective studies. In this study,
we tried to analyze the associations between clinical features and oncogenic somatic mutations in
genome-wide tumor-only samples.

Methods:
Fifty-four tumor-only samples derived from pancreatic cancer patients were used for
whole-exome sequencing. An approach involving SNP filtering of variants included in the
Catalogue of Somatic Mutations in Cancer (COSMIC) database was used to identify oncogenic
somatic mutations. The relationships between oncogenic mutations and clinical features were
analyzed and simultaneously compared with those from the TCGA database.

Results:
By analyzing the mutations from tumor only samples, divergent mutation profiles were
observed in different locations (head vs. body/tail) of pancreatic tumors. The divergences between
pancreatic head and body/tail cancers were also confirmed by the TCGA data. Furthermore,
mutations of several genes were found to be significantly associated with clinical features, such as
pathological stage and the degree of tumor differentiation.

Conclusion:
The results confirmed the efficiency of our approach in identifying oncogenic somatic
mutations from tumor only samples and revealed the associations between somatic mutations and
clinical features in pancreatic cancer.
Identification of Key mRNAs and lncRNAs Associated with the Effects of Anti-TWEAK on Osteosarcoma
Authors: Yang Mingxuan , Zhao Liangtao , Hu Xuchang , Feng Haijun , Kang Xuewen 
Published: June 2020

Background:
Osteosarcoma (OS) is one of the most common primary malignant bone tumors in teenagers.
Emerging studies demonstrated TWEAK and Fn14 were involved in regulating cancer cell differentiation, proliferation,
apoptosis, migration and invasion.

Objective:
The present study identified differently expressed mRNAs and lncRNAs after
anti-TWEAK treatment in OS cells using GSE41828.

Methods:
We identified 922 up-regulated mRNAs, 863 downregulated mRNAs, 29 up-regulated lncRNAs, and 58 down-regulated lncRNAs after anti-TWEAK treatment in OS cells.
By constructing PPI networks, we identified several key proteins involved in anti-TWEAK treatment in OS cells, including
MYC, IL6, CD44, ITGAM, STAT1, CCL5, FN1, PTEN, SPP1, TOP2A, and NCAM1. By constructing lncRNAs coexpression networks, we identified several key lncRNAs, including LINC00623, LINC00944, PSMB8-AS1,
LOC101929787.

Result:
Bioinformatics analysis revealed DEGs after anti-TWEAK treatment in OS were involved in
regulating type I interferon signaling pathway, immune response related pathways, telomere organization, chromatin
silencing at rDNA, and DNA replication. Bioinformatics analysis revealed differently expressed lncRNAs after antiTWEAK treatment in OS were related to telomere organization, protein heterotetramerization, DNA replication, response
to hypoxia, TNF signaling pathway, PI3K-Akt signaling pathway, Focal adhesion, Apoptosis, NF-kappa B signaling
pathway, MAPK signaling pathway, FoxO signaling pathway.

Conclusion: :
This study provided useful information for
understanding the mechanisms of TWEAK underlying OS progression and identifying novel therapeutic markers for OS.
Tapered Optical Fiber Detector for a Red Dye Concentration Measurement
Authors: Polokhin Aleksandr A. , Kharissova Oxana V. , Torres-Martínez Leticia M. , Gerasimenko Alexander Y. , Selvas Romeo , Jiang Jiechao , Kharisov Boris I. 
Published: June 2020

Background:
In this work, a detector based on optical fiber covered with Multi-Wall Carbon
Nanotubes (MWCNTs) was used for sensing and removal of Alizarin from wastewaters. Alizarin
is a strong anionic red dye that is part of the anthraquinone dye group. As a rule, this dye is used in the
textile industry as a coloring agent. Experiments showed a good efficiency of wastewater treatment.
This development could resolve the problem of water contamination with Alizarin red dye.

Methods:
We used a single-mode fiber SMF-28e with a core diameter of 8.2 μm and a cladding diameter
of 125 μm as a base for the tapered optical fiber detector. An MWCNTs array was synthesized on
the tapered optical fiber detector surface by spray pyrolysis Chemical Vapor Deposition (CVD) method
at 800oC for 20 min inside a tubular furnace, using ferrocene solution in toluene as a catalyst precursor.
The formed structure was applied for Alizarin detection in water.

Results:
According to the patent studies, the nanotubes completely covered the optical fiber surface
and the array had a high density with minimal distance between nearby nanotubes. Carbon nanotubes
were oriented along the radius of the optical fiber. The average diameter of carbon nanotubes was 24
nm. The optical absorbance levels increased as the Alizarin concentration increased from 50 mg/L to
1000 mg/L. MWCNTs on the optical fiber tapered section adsorbed the dye molecules from aqueous
solution. Three intensive absorption bands with the wavelength of the 700, 714 and 730 nm appeared
and their intensity increased as the Alizarin concentration increased. The accumulated Alizarin can be
recovered by multiple immersing clean water. This property may make tapered optical fiber detector
reusable and increase the economic expediency of the sensor application.

Conclusions:
The study showed higher Alizarin adsorption efficiency of the tapered optical fiber detector
compared with relative detectors. This structure can be reusable for dye detection. Removal efficiency
for Alizarin reached 98.6%, which makes the tapered optical fiber detector promising for
wastewater treatment and dye elimination.
Intraoral Scanners in Personal Identification of Corpses: Usefulness and Reliability of 3D Technologies in Modern Forensic Dentistry
Authors: Putrino Alessandra , Bruti Valerio , Enrico Marinelli , Costantino Ciallella , Ersilia Barbato , Gabriella Galluccio 
Published: June 2020

Aims:This study aims to verify the applicability of modern dental technologies and their related principles of use to the forensic sciences in the field of personal identification.Background:Personal identification has always had a major role in many legal and administrative actions regarding both living and death beings. The techniques used are much less advanced than the technologies potentially available.Objective:Modern technologies, available to the daily dental clinic practice, as intraoral scanners, combined in particular to the specialist skill in orthodontics, can help redefine the methods of personal identification according to the levels of accuracy, trueness and feasibility greater than those applied in traditional forensic dentistry.Methods:23 corpses (12F;11M) have been selected for intraoral scanning with the Carestream 3500®digital device. The superimposition of initial and late digital models, digital models and radiographs (orthopantomography and full mouth periapical films) has been evaluated to verify the stability of some structures as palatal rugae after death and to assess intraoral scanning as a successful comparative method between antemortem and post-mortem records (digital models or radiographs). Obtained results were subjected to statistical analysis by the t-student test and X-square test with Yates correction (p<0.05).Results:After death, palatal rugae significatively change especially in mouths with restorations/prosthesis/missing teeth. The percentages of correct matching between scans and radiographs are very higher (up 90%; p<0.05).Conclusion:This study has been set up to study and develop new, reliable and fast methods of personal identification that can surpass many of the issues seen with the other techniques by a modern rugoscopy, a modern radiographic-digital comparison and virtual oral autopsy.