bluuebunny/crossref_metadata_2025_split huggingface dataset:
Air and surface contamination in non-health care settings among 641 environmental specimens of 39 COVID-19 cases
Authors: Luo Lei , Liu Dan , Zhang Hao , Li Zhihao , Zhen Ruonan , Zhang Xiru , Xie Huaping , Song Weiqi , Liu Jie , Huang Qingmei , Liu Jingwen , Yang Xingfen , Chen Zongqiu , Mao Chen 
Published: July 2020

AbstractBackgroundLittle is known about the SARS-CoV-2 contamination of environmental surfaces and air in non-health care settings among COVID-19 cases.Methods and findingsWe explored the SARS-CoV-2 contamination of environmental surfaces and air by collecting air and swabbing environmental surfaces among 39 COVID-19 cases in Guangzhou, China. The specimens were tested by RT-PCR testing. The information collected for COVID-19 cases included basic demographic, clinical severity, onset of symptoms, radiological testing, laboratory testing and hospital admission. A total of 641 environmental surfaces and air specimens were collected among 39 COVID-19 cases before disinfection. Among them, 20 specimens (20/641, 3.1%) were tested positive from 9 COVID-19 cases (9/39, 23.1%), with 5 (5/101, 5.0%) positive specimens from 3 asymptomatic cases, 5 (5/220, 2.3%) from 3 mild cases, and 10 (10/374, 2.7%) from 3 moderate cases. All positive specimens were collected within 3 days after diagnosis, and 10 (10/42, 23.8%) were found in toilet (5 on toilet bowl, 4 on sink/faucet/shower, 1 on floor drain), 4 (4/21, 19.0%) in anteroom (2 on water dispenser/cup/bottle, 1 on chair/table, 1 on TV remote), 1 (1/8, 12.5%) in kitchen (1 on dining-table), 1 (1/18, 5.6%) in bedroom (1 on bed/sheet pillow/bedside table), 1 (1/5, 20.0%) in car (1 on steering wheel/seat/handlebar) and 3 (3/20, 21.4%) on door knobs. Air specimens in room (0/10, 0.0%) and car (0/1, 0.0%) were all negative.ConclusionsSARS-CoV-2 was found on environmental surfaces especially in toilet, and could survive for several days. We provided evidence of potential for SARS-CoV-2 transmission through contamination of environmental surfaces.
A single cell atlas of human cornea that defines its development, limbal stem and progenitor cells and the interactions with the limbal niche
Authors: Collin Joseph , Queen Rachel , Zerti Darin , Bojic Sanja , Moyse Nicky , Molina Marina Moya , Yang Chunbo , Reynolds Gary , Hussain Rafiqul , Coxhead Jonathan M , Lisgo Steven , Henderson Deborah , Joseph Agatha , Rooney Paul , Ghosh Saurabh , Connon Che , Haniffa Muzlifah , Figueiredo Francisco , Armstrong Lyle , Lako Majlinda 
Published: July 2020

SummaryTo study the development and composition of human ocular surface, we performed single cell (sc) RNA-Seq at key embryonic, fetal and adult stages and generated the first atlas of the corneal cell types from development to adulthood. Our data indicate that during development, the conjunctival epithelium is the first to be specified from the ocular surface epithelium, followed by the corneal epithelium and the establishment of proliferative epithelial progenitors, which predate the formation of limbal niche by a few weeks. Bioinformatic comparison of adult cell clusters identified GPHA2, a novel cell-surface marker for quiescent limbal stem cells (qLSCs), whose function is to maintain qLSCs self-renewal. Combining scRNA- and ATAC-Seq analysis, we identified multiple upstream regulators for qLSCs and transit amplifying (TA) cells and demonstrated a close interaction between the immune cells and epithelial stem and progenitor cells in the cornea. RNA-Seq analysis indicated loss of qLSCs and acquisition of proliferative limbal basal epithelial progenitor markers duringex vivolimbal epithelial cell expansion, independently of the culture method used. Extending the single cell analyses to keratoconus, we were able to reveal activation of collagenase in the corneal stroma and a reduced pool of TA cells in the limbal epithelium as two key changes underlying the disease phenotype. Our scRNA- and ATAC-Seq data of developing and adult cornea in steady state and disease conditions provide a unique resource for defining pathways/genes that can lead to improvement inex vivoexpansion and differentiation methods for cell based replacement therapies and better understanding and treatment of ocular surface disorders.Key findingsscRNA-Seq of adult human cornea and conjunctiva reveals the signature of various ocular surface cell populationsscRNA-Seq of human developing cornea identifies stage-specific definitions of corneal epithelial, stromal and endothelial layersscRNA-Seq analysis results in identification of novel markers for qLSCs and TA cellsCombined scRNA- and ATAC-Seq analysis reveals key transcriptional networks in qLSCs and TA cells and close interactions with immune cellsExpansion of limbal epithelium results in downregulation of qLSCs and acquisition of proliferative limbal epithelial progenitor markersscRNA-Seq of keratoconus corneas reveals activation of collagenase in the corneal stroma and a reduced pool of TA cells in the limbal epitheliumGraphical abstractSchematic presentation of main techniques and findings presented in this manuscript.
Knockout of Cyp26a1 and Cyp26b1 during post-natal life causes reduced lifespan, dermatitis, splenomegaly and systemic inflammation in mice
Authors: Snyder Jessica M , Zhong Guo , Hogarth Cathryn , Huang Weize , Topping Traci , LaFrance Jeffrey , Palau Laura , Czuba Lindsay C , Griswold Michael , Ghiaur Gabriel , Isoherranen Nina 
Published: July 2020

AbstractAll-trans-retinoic acid (atRA), the active metabolite of vitamin A, is an essential signaling molecule. Global knockout of theatRA clearing enzymes Cyp26a1 or Cyp26b1 is embryonic lethal. In adults, inhibition of Cyp26a1 and Cyp26b1 increasesatRA concentrations and signaling. However, post-natal knockout of Cyp26a1 does not cause a severe phenotype. We hypothesized that Cyp26b1 is the mainatRA clearing Cyp in post-natal mammals. This hypothesis was tested by generating tamoxifen inducible knockout mouse models of Cyp26b1 alone or with Cyp26a1. Both mouse models showed dermatitis, blepharitis and splenomegaly. Histology showed infiltration of inflammatory cells including neutrophils and T-lymphocytes into the skin and hyperkeratosis/hyperplasia of the non-glandular stomach. The mice lacking both Cyp26a1 and Cyp26b1 also failed to gain weight and showed fat atrophy. There were significant changes in vitamin A homeostasis demonstrating the paramount role of Cyp26b1 in regulating retinoid homeostasis in post-natal life.
Repeat SARS-CoV-2 Testing Models for Residential College Populations
Authors: Chang Joseph T. , Crawford Forrest W. , Kaplan Edward H. 
Published: July 2020

AbstractResidential colleges are considering re-opening under uncertain futures regarding the COVID-19 pandemic. We consider repeat SARS-CoV-2 testing models for the purpose of containing outbreaks in the residential campus community. The goal of repeat testing is to detect and isolate new infections rapidly to block transmission that would otherwise occur both on and off campus. The models allow for specification of aspects including scheduled on-campus resident screening at a given frequency, test sensitivity that can depend on the time since infection, imported infections from off campus throughout the school term, and a lag from testing until student isolation due to laboratory turnaround and student relocation delay. For early- (late-) transmission of SARS-CoV-2 by age of infection, we find that weekly screening cannot reliably contain outbreaks with reproductive numbers above 1.4 (1.6) if more than one imported exposure per 10,000 students occurs daily. Screening every three days can contain outbreaks providing the reproductive number remains below 1.75 (2.3) if transmission happens earlier (later) with time from infection, but at the cost of increased false positive rates requiring more isolation quarters for students testing positive. Testing frequently while minimizing the delay from testing until isolation for those found positive are the most controllable levers for preventing large residential college outbreaks. Aweb appthat implements model calculations is available to facilitate exploration and consideration of a variety of scenarios.
Severe SAR-CoV-2 infection in humans is defined by a shift in the serum lipidome resulting in dysregulation of eicosanoid immune mediators
Authors: Schwarz Benjamin , Sharma Lokesh , Roberts Lydia , Peng Xiaohua , Bermejo Santos , Leighton Ian , Massana Arnau Casanovas , Farhadian Shelli , Ko Albert I. ,   Yale IMPACT Team, Dela Cruz Charles S. , Bosio Catharine M. 
Published: July 2020

Introductory ParagraphThe COVID-19 pandemic has affected more than 10 million people worldwide with mortality exceeding half a million patients. Risk factors associated with severe disease and mortality include advanced age, hypertension, diabetes, and obesity.1 Clear mechanistic understanding of how these comorbidities converge to enable severe infection is lacking. Notably each of these risk factors pathologically disrupts the lipidome and this disruption may be a unifying feature of severe COVID-19.1-7 Here we provide the first in depth interrogation of lipidomic changes, including structural-lipids as well as the eicosanoids and docosanoids lipid mediators (LMs), that mark COVID-19 disease severity. Our data reveal that progression from moderate to severe disease is marked by a loss of specific immune regulatory LMs and increased pro-inflammatory species. Given the important immune regulatory role of LMs, these data provide mechanistic insight into the immune balance in COVID-19 and potential targets for therapy with currently approved pharmaceuticals.8
Comparison of SARS-CoV-2 serological tests with different antigen targets
Authors: Coste Alix T. , Jaton Katia , Papadimitriou-Olivgeris Matthaios , Greub Gilbert , Croxatto Antony 
Published: July 2020

AbstractBackgroundThese last months, dozens of SARS-CoV-2 serological tests have become available with varying performances. A major effort was completed to compare 17 serological tests.MethodsIn a preliminary phase, we compared 17 IgG, IgM, IgA and pan Ig serological tests including ELISA, LFA, CLIA and ECLIA on a panel of 182 sera, comprising 113 sera from hospitalized patients with a positive RT-PCR, and 69 sampled before 1stNovember 2019, expected to give a positive and negative results, respectively. In a second phase, the five best performing and most available tests were further evaluated on a total of 582 sera (178 and 404 expected positive and negative, respectively), allowing the assessment of 20 possible cross-reactions with other virus.ResultsIn the preliminary phase, among eight IgG/pan-Ig ELISA or CLIA/ECLIA tests, four had a sensitivity and specificity above 90% and 98% respectively, and on six IgM/IgA tests, only one was acceptable. Only one LFA test on three showed good performances for both IgG and IgM. For all the tests IgM and IgG aroused concomitantly. In the second phase, no tests showed particular cross-reaction. We observed an important heterogeneity in the development of the antibody response, and that anti-nucleocapside (anti-N) antibodies appeared earlier than the anti-spike (anti-S) proteins.ConclusionsThe identified SARS-CoV-2 serology tests may be used for the diagnostic of CoviD-19 for negative RT-PCR patients presenting severe to mild suggestive symptoms or particular clinical presentation. Detection of both anti-N and anti-S could be complementary to increase the sensitivity of the analysis.
Long-Read cDNA Sequencing Enables a “Gene-Like” Transcript Annotation of Transposable Elements
Authors: Panda Kaushik , Slotkin R. Keith 
Published: July 2020

Abstract
Transcript-based annotations of genes facilitate both genome-wide analyses and detailed single-locus research. In contrast, transposable element (TE) annotations are rudimentary, consisting of information only on TE location and type. The repetitiveness and limited annotation of TEs prevent the ability to distinguish between potentially functional expressed elements and degraded copies. To improve genome-wide TE bioinformatics, we performed long-read sequencing of cDNAs from Arabidopsis (Arabidopsis thaliana) lines deficient in multiple layers of TE repression. These uniquely mapping transcripts were used to identify the set of TEs able to generate polyadenylated RNAs and create a new transcript-based annotation of TEs that we have layered upon the existing high-quality community standard annotation. We used this annotation to reduce the bioinformatic complexity associated with multimapping reads from short-read RNA sequencing experiments, and we show that this improvement is expanded in a TE-rich genome such as maize (Zea mays). Our TE annotation also enables the testing of specific standing hypotheses in the TE field. We demonstrate that inaccurate TE splicing does not trigger small RNA production, and the cell more strongly targets DNA methylation to TEs that have the potential to make mRNAs. This work provides a transcript-based TE annotation for Arabidopsis and maize, which serves as a blueprint to reduce the bioinformatic complexity associated with repetitive TEs in any organism.
Feasibility study of interferometric phase-contrast X-ray imaging using the hard-X-ray free-electron laser of the SPring-8 Angstrom Compact Free-Electron Laser
Authors: Yoneyama Akio , Baba Rika , Takamatsu Daiko , Kamezawa Chika , Inoue Ichiro , Osaka Taito , Owada Shigeki , Yabashi Makina 
Published: July 2020

Aiming for the fine observation of fast physical phenomena such as phonon propagation and laser ablation, phase-contrast X-ray imaging combined with a crystal X-ray interferometer and the X-ray free-electron laser (XFEL) of the SPring-8 Angstrom Compact Free-Electron Laser has been developed. An interference pattern with 70% visibility was obtained by single-shot exposure with a 15 keV monochromated XFEL. In addition, a phase map of an acrylic wedge was successfully obtained using the fringe scanning method.
Delivery mechanism on homeownership education among millennials in Kuala Lumpur
Authors: Zyed Zafirah Al Sadat , Low Chooi Sien , Tedong Peter Aning 
Published: July 2020

Purpose
Millennials are considered the best group to intervene in terms of homeownership education as they are of working age and can earn monthly income. However, there is a concern about the affordability of millennials to purchase a house that will influence their decision in terms of purchasing a house. Further understanding of the home-buying process allows millennials to avoid dishonest and irresponsible sellers, as purchasing a house involves a large sum of money. The purpose of this study is to suggest a suitable delivery mechanism to increase awareness of the home-buying process among millennials in Kuala Lumpur.

Design/methodology/approach
This study adopts a quantitative method to analyse sets of questionnaire survey that were distributed at purposive random sampling in Kuala Lumpur. This study approached respondents with pre-set criteria, which include the respondents to be between 18 and 38 years old, interested to purchase a house and to have at least an initiatory understanding of the home-buying process.

Findings
The findings suggested that there is considerably low awareness of the home-buying process among millennials. However, it is important to note that among the home-buying process, millennials are most aware in the preliminary phase, which is information gathering. Based on these findings, the best delivery mechanism to increase awareness is through online games and training courses, followed by home counselling at a local housing agency.

Practical implications
This study contributes to government agencies and policymakers to interact with society, as education is one of the best methods. It will further enhance their efforts to ensure that the society is well equipped with useful knowledge to avoid the rueful decision of purchasing their first house.

Originality/value
This paper highlights homeownership education by tackling the issue of extensive knowledge of the home-buying process. This is crucial to the foundation of homeownership education, as it reflects the efforts of government agencies and policymakers to ensure homebuyers’ rights are protected in the housing market. This paper will benefit not only policymakers and decision-makers but also first time homebuyers.
Workplace ostracism and work-to-family conflict among female employees: moderating role of perceived organisational support
Authors: Choi Youngkeun 
Published: July 2020

Purpose
Based on the conservation of resources theory, this study aims to develop and test the relationship between workplace ostracism and the work-to-family conflict of a female employee. And, it assumes that the direct link between workplace ostracism and the work-to-family conflict of a female employee is moderated by perceived organizational support.

Design/methodology/approach
For this, this study used a survey method and multiple regression analyses with multi-source data from 226 Korean female employees.

Findings
The results suggest the following. First, workplace ostracism was positively associated with the work-to-family conflict of a female employee. Second, there was a stronger positive relationship between workplace ostracism and work-to-family conflict for female employees with low as opposed to those with high levels of perceived organizational support.

Originality/value
To the best of author’s knowledge, this study is the first one to examine the moderating effect of perceived organizational support on the relationship between workplace ostracism and the work-to-family conflict of a female employee.
Firm collaborative capability and new product development performance: the mediating role of heterogeneous knowledge acquisition
Authors: Zhang Feng , Zhu Lei 
Published: July 2020

PurposeFrom the dynamic capability perspective, the aim of this paper is to develop a conceptual framework for collaborative capability, including scanning, relational skills and adaptation and to test the impact of each dimension on the acquisition of technological and marketing knowledge from partners. The conceptual framework also suggests that these two types of knowledge acquisition have different impacts on new product development (NPD) creativity and speed.Design/methodology/approachThis study builds a theoretical framework and tests it with survey data from 289 Chinese manufacturing firms.FindingsFirms with strong collaborative capability are identified to gain better knowledge acquisition from their partners and achieve higher NPD performance. While acquired technological knowledge has a greater effect on NPD creativity than acquired marketing knowledge, the latter has a greater effect on NPD speed. In addition, these two types of knowledge acquisition form different mediating paths between collaborative capability and NPD performance.Practical implicationsThis study not only underlines the important role of collaborative capability in facilitating knowledge acquisition, which in turn improves NPD performance, but also suggests that decision-makers should note the different roles of technological knowledge and market knowledge in influencing NPD performance.Originality/valueThese findings enrich the understanding of how firms enhance NPD performance by developing collaborative capability in a major emerging economy (i.e. China). In addition to demonstrating the differential effects of heterogeneous knowledge acquisition on NPD performance, different pathways of mediation through knowledge acquisition are also identified in the relationship between collaborative capability and NPD outcomes.
The nature of individual experiential knowledge in internationalizing SMEs: pitfalls of superstitious learning and the need for wisdom
Authors: Hånell Sara Melén , Rovira Nordman Emilia , Mattsson Lars-Gunnar 
Published: July 2020

PurposeThis study addresses the research question: How does the experiential knowledge, superstitious knowledge and the wisdom of CEOs influence the internationalization behaviour of SMEs?Design/methodology/approachAn exploratory qualitative study is used. Longitudinal case studies of two Swedish life science companies are analysed.FindingsAn individual's prior experiential knowledge influence the newly started SME's market commitments and internationalization behaviour. Such prior experiences can enable early and rapid resource commitments in the newly started SMEs. Relying upon such prior experiential knowledge in deciding upon the company's market commitments however heightens the risk of superstitious learning. The findings illustrate how wisdom can work as an antidote to superstitious learning. Wisdom lures even experienced CEOs away from believing they know more than they actually know.Research limitations/implicationsThe study contributes to extend the Uppsala model by incorporating the role of individual-level experiential knowledge. The study also adds value to the literature on small firm internationalization by providing propositions for how the prior knowledge of individual key decision makers influences SMEs' internationalization behaviour. The propositions provide new input to the ongoing discussion in the literature and help to guide future research.Originality/valueGiven the fact that the Uppsala model is centred upon a firm-level view on experiential knowledge, our theoretical understanding is still limited regarding how individual-level experiential knowledge influences the internationalization behaviour of SMEs. This study addresses calls for research on how individuals' prior knowledge influences small-firm internationalization.
An integrated decision-making model for supplier evaluation in public healthcare system: the case study of a Spanish hospital
Authors: Yazdani Morteza , Torkayesh Ali Ebadi , Chatterjee Prasenjit 
Published: July 2020

PurposeIn this study, an integrated decision-making model consisting of decision-making trial and evaluation laboratory (DEMATEL), best worst method (BWM) and a modified version of evaluation based on distance from average solution (EDAS) methods is proposed for supplier selection problem in a public procurement system considering sustainable development goals.Design/methodology/approachDEMATEL and BWM methods are used to determine weights of the criteria that are defined for the supplier selection problem. Weight aggregation method is applied to combine the weights obtained from these two methods. A modified version of EDAS method is then used in order to rank the alternative suppliers.FindingsThe proposed decision-making model is investigated for a supplier selection problem for a hospital in Spain. The validity of the results is checked using comparison with other decision-making methods and several performance analysis tests.Practical implicationsThe proposed multi-criteria decision-making (MCDM) model contributes to the healthcare supply chain management (SCM) and aims to lead the policy makers in selecting the best supplier.Originality/valueThere is no such study that combines DEMATEL and BWM together for weight generation. The application of the modified EDAS method is also new. In real time situations, the decision experts may confront to the difficulty of using BWM while identifying the best and the worst criteria choices. The idea of using DEMATEL is to aid the experts to make them enable in distinguishing between the best/worst criteria and handle BWM easily.