bluuebunny/crossref_metadata_2025_split huggingface dataset:
An arthroscopic repair technique for proximal anterior cruciate tears in children to restore active function and avoid growth disturbances
Authors: Turati Marco , Rigamonti Luca , Zanchi Nicolò , Piatti Massimiliano , Gaddi Diego , Gorla Massimo , Omeljaniuk Robert J. , Courvoisier Aurelien , Bigoni Marco 
Published: January 2021

AbstractPurposeThe aim of this study was to assess midterm clinical outcomes in Tanner 1–2 patients with proximal anterior cruciate ligament (ACL) tears following arthroscopic-surgical repair using an absorbable or an all-suture anchor.MethodsFourteen (9.2 ± 2.9 years-old) of 19 skeletally immature patients reached the 2 years of clinical follow-up. Physical examinations included the Lachman test, Pivot-shift test, One-leg Hop test, Pedi-IKDC as well as Lysholm and Tegner activity scores; knee stability was measured with a KT-1000 arthrometer. Overall re-rupture rates were also evaluated in all operated patients.ResultsAt 2 years post-surgery, the Lysholm score was 93.6 ± 4.3 points, and the Pedi-IKDC score was 95.7 ± 0.1. All patients returned to the same sport activity level as prior to ACL lesion within 8.5 ± 2.9 months, with one exception who reported a one-point reduction in their Tegner Activity score. No leg-length discrepancies or malalignments were observed. Four patients presented grade 1 Lachman scores, and of these, three presented grade 1 (glide) score at Pivot-shift; clinical stability tests were negative for all other patients. Anterior tibial shift showed a mean side-to-side difference of 2.2 mm (range 1–3 mm). The One-leg Hop test showed lower limb symmetry (99.9% ± 9.5) with the contralateral side. Overall, 4 out of 19 patients presented a re-rupture of the ACL with a median time between surgery and re-rupture of 3.9 years (range 1–7).ConclusionThis surgical technique efficiently repairs proximal ACL tears, leading to a restoration of knee stability and a quick return to an active lifestyle, avoiding growth plate disruption.Level of evidenceIV.
Maternal High-Fat Diet Induces Long-Lasting Defects in Bone Structure in Rat Offspring Through Enhanced Osteoclastogenesis
Authors: Kushwaha Priyanka , Khambadkone Seva G. , Li Mengni , Goodman Ethan J. , Aravindan Nandini , Riddle Ryan C. , Tamashiro Kellie L. K. 
Published: January 2021

AbstractMaternal stressors during the prenatal and perinatal periods are associated with increased susceptibility for and severity of chronic disease phenotypes in adult offspring. In this study, we used a rat model of maternal high-fat diet (HFD) exposure during pregnancy and lactation to investigate the impact on skeletal homeostasis in offspring. In the distal femur, young male and female offspring (up to 3 weeks of age) from dams fed a HFD exhibited marked increases in trabecular bone volume relative to offspring from dams fed a chow diet, but this was followed by sustained bone loss. By 15 weeks of age, male offspring of HFD fed dams exhibited a 33% reduction in trabecular bone volume fraction that histomorphometric analyses revealed was due to a nearly threefold increase in the abundance of bone-resorbing osteoclasts, while there were no differences between female control and HFD offspring by 15 weeks of age. The osteoblastic differentiation of male offspring-derived bone marrow stromal cells was not affected by maternal diet. However, osteoclastic precursors isolated from the male offspring of HFD fed dams exhibited enhanced differentiation in vitro, forming larger osteoclasts with higher expression of the fusion marker DC-STAMP. This effect appears to be mediated by a cell autonomous increase in the sensitivity of precursors to RANKL. Taken together, these results suggest that maternal stressors like HFD exposure have persistent consequences for the skeletal health of offspring that may ultimately lead to a predisposition for osteopenia/osteoporosis.
Prognostic value of ASXL1 mutations in patients with primary myelofibrosis and its relationship with clinical features: a meta-analysis
Authors: Wang Ziqing , Liu Weiyi , Wang Mingjing , Li Yujin , Wang Xueying , Yang Erpeng , Ming Jing , Quan Richeng , Hu Xiaomei 
Published: January 2021

AbstractAdditional sex combs like 1 (ASXL1) mutations are one of the most common molecular biological abnormalities in patients with primary myelofibrosis (PMF), and the effect of these mutations on prognosis remains controversial. Hence, we conducted a meta-analysis to assess the prognostic value and clinical characteristics of ASXL1 mutations in PMF patients. Eligible studies were systematically searched from PubMed, Embase, and the Cochrane Library. We extracted the hazard ratios (HRs) and their 95% confidence intervals (CIs) of overall survival (OS) and leukemia-free survival (LFS), the number of patients transformed to acute leukemia, and clinical characteristics to carry out a meta-analysis by fixed effect model or random effect model according to the heterogeneity between studies. A total of 4501 PMF patients from 16 cohorts of 14 studies were included in this meta-analysis. The results revealed that ASXL1 mutations might predict a shorter OS (HR = 2.30, 95% CI: 1.79–2.94, P < 0.00001) and a higher probability of transformation to acute leukemia (LFS: HR = 1.77, 95% CI: 1.30–2.42, P = 0.0003; the rate of acute leukemia transformation: OR = 2.06, 95% CI: 1.50–2.83, P < 0.00001). Furthermore, ASXL1 mutations were correlated with patients older than 65 years old, male, a lower level of platelet counts, and a higher risk of the international prognostic score system. These findings indicate that ASXL1 mutations have a significant adverse impact on the prognosis of PMF patients and may contribute to risk stratification and prognostic assessment for PMF patients.
FOLFIRINOX in advanced pancreatic cancer patients with the double-variant type of UGT1A1 *28 and *6 polymorphism: a multicenter, retrospective study
Authors: Umemoto Kumiko , Takahashi Hideaki , Morizane Chigusa , Yamada Ikuhiro , Shimizu Satoshi , Shioji Kazuhiko , Yoshida Yukio , Motoya Masayo , Mizuno Nobumasa , Kojima Yasushi , Terashima Takeshi , Uesugi Kazuhiro , Ueno Makoto , Furuse Junji , Akimoto Tetsuo , Ikeda Masafumi 
Published: January 2021

Abstract
Background
UGT1A1 *28 and *6 polymorphism is associated with reduced enzyme activity and severe toxicities of irinotecan, especially in patients with homozygous or heterozygous for UGT1A1*28 or *6 polymorphism for both UGT1A1*28 and *6 (double-variant-type of UGT1A1 polymorphism, UGT1A1-DV). FOLFIRINOX is one of the standard treatments for metastatic pancreatic cancer (PC). The optimal dose of irinotecan as a component of the FOLFIRINOX has not been established yet for patients with UGT1A1-DV.

Patients and methods
Advanced PC patients with UGT1A1-DV who had received at least one cycle of FOLFIRINOX from December 2013 to March 2016 were collected retrospectively conducted at multicenter in Japan. We evaluated the patient characteristics, efficacy and safety of FOLFIRINOX and investigate the optimal initial dose of irinotecan in Japanese advanced PC patients with UGT1A1-DV.

Results
A total of 31 patients were enrolled. Grade 4 neutropenia was seen more frequently (67%; 4/6) in patients who had received irinotecan at an initial dose of  ≥ 150 mg/m2 than in those who had received the drug at an initial dose of  ≤ 120 mg/m2 (20%; 5/24). The response rate (RR) and progression-free survival (PFS) in patients given irinotecan of  ≤ 120 mg/m2 were 21.4% and 8.1 months, respectively, which were consistent with previous report for patients without UGT1A1-DV.

Conclusion
Based on our findings, we recommend that in Japanese advanced PC patients with UGT1A1- DV treated with FOLFIRINOX, irinotecan be administered at an initial dose of  ≤ 120 mg/m2.
A study of predictability of coupled ocean–atmosphere system using attractor radius and global attractor radius
Authors: Zhao Haoran , Zhang Shaoqing , Li Jianping , Ma Youwei 
Published: January 2021

AbstractWhile ocean–atmosphere coupled models play an increasingly important role in weather-climate simulation and prediction, the predictability theory based on an atmosphere-only model has significant limitations in interpreting prediction results and guiding predictability studies. Here we use a conceptual ocean–atmosphere coupled model that describes the typical interactions of a synoptic-scale atmosphere with a seasonally-interannually varying upper ocean as well as a deep ocean that varies on decadal timescales to systematically study the predictability of a coupled system. Moving from an atmosphere-only system to an ocean–atmosphere coupled system, the initial-value predictability problem becomes a joint initial-value and boundary-value problem. Although the coupling process increases the uncertainties of the boundary, ocean signals with longer timescales are added to the atmosphere system, thus increasing its predictability. We then investigate the predictability characteristics of the National Centers for Environmental Prediction coupled Climate Forecast System (CFS) and the uncoupled Global Ensemble Forecast System (GEFS). In the coupled CFS system, the practical predictability limit of the lower troposphere is significantly longer than in the uncoupled GEFS due to the contribution of low-frequency boundary signals from air-sea interactions. While further deep and thorough examination is necessary for understanding ocean predictability in the climate system, a preliminary discussion for the predictability of the upper and deep oceans within a coupled ocean–atmosphere framework is also presented in this study.
Uniportal Versus Multiportal Video-Assisted Thoracoscopic Lobectomy for Lung Cancer: An Updated Meta-analysis
Authors: Magouliotis Dimitrios E. , Fergadi Maria P. , Spiliopoulos Kyriakos , Athanassiadi Kalliopi 
Published: January 2021

Abstract
Objective
We reviewed the available literature on patients with lung cancer undergoing either uniportal (UVATS) or multiport video-assisted thoracoscopic surgery (MVATS).

Methods
Original research studies that evaluated perioperative and long-term outcomes of UVATS versus MVATS were identified, from January 1990 to April 2020. The perioperative, along with the oncologic and long-term survival outcomes, were calculated according to either a fixed or a random effect model, appropriately. The Q statistics and I2 statistic were used to test for heterogeneity among the studies.

Results
Twenty studies were included, incorporating a total of 1,469 patients treated with UVATS and 3,231 treated with MVATS. The incidence of complications was lower in patients treated with UVATS [OR: 0.76 (95% CI 0.62, 0.93); p = 0.008]. The chest tube duration was significantly lower in the UVATS group (WMD: − 0.63 [95% CI − 1.03, − 0.23]; p = 0.002). Length of hospital stay (L.O.S.) was also lower in the UVATS patient group (WMD: − 0.54 [− 0.94, − 0.13]; p = 0.009), along with postoperative pain [WMD: − 0.57 (95% CI − 0.97, − 0.18); p = 0.004]. No significant differences were found regarding the mean operative time (M.O.T.), mean blood loss, the number of resected lymph nodes, the 30-day mortality, along with the survival at 1 and 3 years postoperatively.

Conclusions
The present meta-analysis indicates that UVATS is associated with enhanced outcomes in patients undergoing surgery for lung cancer. Well-designed, randomized studies, comparing UVATS to MVATS, are necessary to further assess their long-term clinical outcomes.
TGF-β1 increases permeability of ciliated airway epithelia via redistribution of claudin 3 from tight junction into cell nuclei
Authors: Schilpp Carolin , Lochbaum Robin , Braubach Peter , Jonigk Danny , Frick Manfred , Dietl Paul , Wittekindt Oliver H. 
Published: January 2021

AbstractTGF-β1 is a major mediator of airway tissue remodelling during atopic asthma and affects tight junctions (TJs) of airway epithelia. However, its impact on TJs of ciliated epithelia is sparsely investigated. Herein we elaborated effects of TGF-β1 on TJs of primary human bronchial epithelial cells. We demonstrate that TGF-β1 activates TGF-β1 receptors TGFBR1 and TGFBR2 resulting in ALK5-mediated phosphorylation of SMAD2. We observed that TGFBR1 and -R2 localize specifically on motile cilia. TGF-β1 activated accumulation of phosphorylated SMAD2 (pSMAD2-C) at centrioles of motile cilia and at cell nuclei. This triggered an increase in paracellular permeability via cellular redistribution of claudin 3 (CLDN3) from TJs into cell nuclei followed by disruption of epithelial integrity and formation of epithelial lesions. Only ciliated cells express TGF-β1 receptors; however, nuclear accumulations of pSMAD2-C and CLDN3 redistribution were observed with similar time course in ciliated and non-ciliated cells. In summary, we demonstrate a role of motile cilia in TGF-β1 sensing and showed that TGF-β1 disturbs TJ permeability of conductive airway epithelia by redistributing CLDN3 from TJs into cell nuclei. We conclude that the observed effects contribute to loss of epithelial integrity during atopic asthma.
Comparative transcriptomic and metabolomic analyses of carotenoid biosynthesis reveal the basis of white petal color in Brassica napus
Authors: Jia Ledong , Wang Junsheng , Wang Rui , Duan Mouzheng , Qiao Cailin , Chen Xue , Ma Guoqiang , Zhou Xintong , Zhu Meichen , Jing Fuyu , Zhang Shengsen , Qu Cunmin , Li Jiana 
Published: January 2021

AbstractMain conclusionThe molecular mechanism underlying white petal color inBrassica napuswas revealed by transcriptomic and metabolomic analyses.AbstractRapeseed (Brassica napusL.) is one of the most important oilseed crops worldwide, but the mechanisms underlying flower color in this crop are known less. Here, we performed metabolomic and transcriptomic analyses of the yellow-flowered rapeseed cultivar ‘Zhongshuang 11’ (ZS11) and the white-flowered inbred line ‘White Petal’ (WP). The total carotenoid contents were 1.778-fold and 1.969-fold higher in ZS11 vs. WP petals at stages S2 and S4, respectively. Our findings suggest that white petal color in WP flowers is primarily due to decreased lutein and zeaxanthin contents. Transcriptome analysis revealed 10,116 differentially expressed genes with a fourfold or greater change in expression (P-value less than 0.001) in WP vs. ZS11 petals, including 1,209 genes that were differentially expressed at four different stages and 20 genes in the carotenoid metabolism pathway.BnNCED4b, encoding a protein involved in carotenoid degradation, was expressed at abnormally high levels in WP petals, suggesting it might play a key role in white petal formation. The results of qRT-PCR were consistent with the transcriptome data. The results of this study provide important insights into the molecular mechanisms of the carotenoid metabolic pathway in rapeseed petals, and the candidate genes identified in this study provide a resource for the creation of newB. napusgermplasms with different petal colors.
Visual search in virtual 3D space: the relation of multiple targets and distractors
Authors: Plewan Thorsten , Rinkenauer Gerhard 
Published: January 2021

AbstractVisual search and attentional alignment in 3D space are potentially modulated by information in unattended depth planes. The number of relevant and irrelevant items as well as their spatial relations may be regarded as factors which contribute to such effects. On a behavioral level, it might be different whether multiple distractors are presented in front of or behind target items. However, several studies revealed that attention cannot be restricted to a single depth plane. To further investigate this issue, two experiments were conducted. In the first experiment, participants searched for (multiple) targets in one depth plane, while non-target items (distractors) were simultaneously presented in this or another depth plane. In the second experiment, an additional spatial cue was presented with different validities to highlight the target position. Search durations were generally shorter when the search array contained two additional targets and were markedly longer when three distractors were displayed. The latter effect was most pronounced when a single target and three distractors coincided in the same depth plane and this effect persisted even when the target position was validly cued. The study reveals that the depth relation of target and distractor stimuli was more important than the absolute distance between these objects. Furthermore, the present findings suggest that within an attended depth plane, irrelevant information elicits strong interference. In sum, this study provides further evidence that allocation of attention is a flexible process which may be modulated by a variety of perceptual and cognitive factors.
Mesenchymal stem cell therapy in hypertrophic and keloid scars
Authors: Bojanic Christine , To Kendrick , Hatoum Adam , Shea Jessie , Seah K. T. Matthew , Khan Wasim , Malata Charles M. 
Published: January 2021

Abstract
Scars are the normal outcome of wound repair and involve a co-ordinated inflammatory and fibrotic process. When a scar does not resolve, uncontrolled chronic inflammation can persist and elicits excessive scarring that leads to a range of abnormal phenotypes such as hypertrophic and keloid scars. These pathologies result in significant impairment of quality of life over a long period of time. Existing treatment options are generally unsatisfactory, and there is mounting interest in innovative cell-based therapies. Despite the interest in mesenchymal stem cells (MSCs), there is yet to be a human clinical trial that investigates the potential of MSCs in treating abnormal scarring. A synthesis of existing evidence of animal studies may therefore provide insight into the barriers to human application. The aim of this PRISMA systematic review was to evaluate the effectiveness of MSC transplantation in the treatment of hypertrophic and keloid scars in in vivo models. A total of 11 case-control studies were identified that treated a total of 156 subjects with MSCs or MSC-conditioned media. Ten studies assessed hypertrophic scars, and one looked at keloid scars. All studies evaluated scars in terms of macroscopic and histological appearances and most incorporated immunohistochemistry. The included studies all found improvements in the above outcomes with MSC or MSC-conditioned media without complications. The studies reviewed support a role for MSC therapy in treating scars that needs further exploration. The transferability of these findings to humans is limited by factors such as the reliability and validity of the disease model, the need to identify the optimal MSC cell source, and the outcome measures employed.
Consequences of vertical basic wood density variation on the estimation of aboveground biomass with terrestrial laser scanning
Authors: Demol Miro , Calders Kim , Krishna Moorthy Sruthi M. , Van den Bulcke Jan , Verbeeck Hans , Gielen Bert 
Published: January 2021

Abstract
Key message
Stump-to-tip trends in basic wood density complicate the conversion of tree volume into aboveground biomass. We use 3D tree models from terrestrial laser scanning to obtain tree-level volume-weighted wood density.

Abstract
Terrestrial laser scanning (TLS) is used to generate realistic 3D tree models that enable a non-destructive way of quantifying tree volume. An accurate value for basic wood density is required to convert tree volume into aboveground biomass (AGB) for forest carbon assessments. However, basic density is characterised by high inter-, intra-species and within-tree variability and a likely source of error in TLS-derived biomass estimates. Here, 31 adult trees of 4 important European timber species (Fagus sylvatica, Larix decidua, Pinus sylvestris, Fraxinus excelsior) were scanned using TLS and then felled for several basic wood density measurements. We derived a reference volume-weighted basic density (ρw) by combining volume from 3D tree models with destructively assessed vertical density profiles. We compared this to basic density retrieved from a single basal disc over bark (ρbd), two perpendicular pith-to-bark increment cores at breast height (ρic), and sourcing the best available local basic wood density from publications. Stump-to-tip trends in basic wood density caused site-average woody AGB estimation biases ranging from −3.3 to + 7.8% when using ρbd and from −4.1 to + 11.8% when using ρic. Basic wood density from publications was in general a bad predictor for ρw as the bias ranged from −3.2 to + 17.2%, with little consistency across different density repositories. Overall, our density-attributed biases were similar to several recently reported biases in TLS-derived tree volume, leading to potentially large compound errors in biomass assessments with TLS if patterns of vertical basic wood density variation are not properly accounted for.
Improvement of similarity–diversity trade-off in recommender systems based on a facility location model
Authors: Panteli Antiopi , Boutsinas Basilis 
Published: January 2021

AbstractThere is a growing interest in the offering of novel alternative choices to users of recommender systems. These recommendations should match the target query while at the same time they should be diverse with each other in order to provide useful alternatives to the user, i.e., novel recommendations. In this paper, the problem of extracting novel recommendations, under the similarity–diversity trade-off, is modeled as a facility location problem. The results from tests in the benchmark Travel Case Base were satisfactory when compared to well-known recommender techniques, in terms of both similarity and diversity. It is shown that the proposed method is flexible enough, since a parameter of the adopted facility location model constitutes a regulator for the trade-off between similarity and diversity. Also, our work can broaden the perspectives of the interaction and combination of different scientific fields in order to achieve the best possible results.
Association between maternal triglycerides and disturbed glucose metabolism in pregnancy
Authors: Eppel Daniel , Feichtinger Michael , Lindner Tina , Kotzaeridi Grammata , Rosicky Ingo , Yerlikaya-Schatten Guelen , Eppel Wolfgang , Husslein Peter , Tura Andrea , Göbl Christian S. 
Published: January 2021

Abstract
Aims
Dyslipidemia in pregnancy is associated with adverse pregnancy outcomes as elevated triglycerides might be considered as a risk factor for hyperglycemia and gestational diabetes. As only a few studies have addressed the association between maternal triglycerides and glucose metabolism, we aimed to explore the pathophysiologic associations of moderate hypertriglyceridemia and maternal glucose metabolism in pregnancy.

Methods
Sixty-seven pregnant women received a detailed metabolic characterization at 12+0–22+6 weeks of gestation by an extended 2h-75g OGTT (oral glucose tolerance test); with measurements of glucose, insulin and C-peptide at fasting and every 30 min after ingestion and assessment of triglycerides at fasting state. All examinations were repeated at 24+0–27+6 weeks of gestation.

Results
Elevated triglycerides in early gestation were associated with insulin resistance and β-cell dysfunction. Mean glucose concentrations during the OGTT in early pregnancy were already higher in women with hypertriglyceridemia as compared to women with triglycerides in the normal range. A higher degree of insulin resistance and increased OGTT glucose levels were also observed when metabolic assessments were repeated between 24 and 28 weeks of gestation. Of note, elevated triglycerides at early gestation were associated with development of gestational diabetes by logistic regression (odds ratio: 1.16, 95%CI: 1.03–1.34, p=0.022 for an increase of 10 mg/dl).

Conclusions
Hypertriglyceridemia at the start of pregnancy is closely related to impaired insulin action and β-cell function. Women with hypertriglyceridemia have higher mean glucose levels in early- and mid-gestation. Pregnant women with elevated triglycerides in early pregnancy are at increased risk of developing gestational diabetes.