bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comprehensive molecular profiling of intrahepatic cholangiocarcinoma in the Chinese population and therapeutic experience
Authors: Wang Longrong , Zhu Hongxu , Zhao Yiming , Pan Qi , Mao Anrong , Zhu Weiping , Zhang Ning , Lin Zhenhai , Zhou Jiamin , Wang Yilin , Zhang Yongfa , Wang Miao , Feng Yun , He Xigan , Xu Weiqi , Wang Lu 
Published: April 2020

Abstract
PURPOSE: The genomic alterations of intrahepatic cholangiocarcinoma (ICC) in the Chinese population have not been fully revealed. Molecular profiling may provide a reference for clinical management, especially targeted therapy. METHODS: A retrospective study was conducted in 122 ICC patients. All patients had results of next-generation sequencing (NGS) assays that analyzed 417 cancer-associated genes. The genetic characteristics, clinical management and therapeutic responses were analyzed. RESULTS: The most commonly mutated genes were TP53 (34%), KRAS (25%) and ARID1A (17%). Targeted agents were used referring to molecular profiling, with a combination of chemotherapy. Twenty-two patients with wild-type KRAS/NRAS/BRAF were treated with cetuximab. The disease control and response rates were 78% and 47%, respectively, which were higher than those achieved with chemotherapy alone (72% and 11%, P=0.16). Fifty-four patients underwent anti-VEGF treatment with bevacizumab. The disease control and response rates were 85% and 60%, respectively. Better therapeutic efficiency (P=0.001) and longer progression-free survival (PFS; 15.4 vs 6.7 months, P=0.04) were observed in the bevacizumab-treated group than in the chemotherapy alone group. The PFS of ten patients who underwent hepatectomy after combined treatment with chemotherapy and bevacizumab was longer than that of 139 patients who underwent surgical treatment (28.9 vs 18.0 months, P=0.03). Two patients (1.6%) had signatures of microsatellite instability (MSI-H), and both benefited from immunotherapy. CONCLUSIONS: This study provides an overview of genetic alterations in Chinese ICC patients and indicates the potential clinical implications for NGS-based personalized therapies.
Suppression of Rcn3 in the alveolar epithelial cells may have beneficial effect against COPD
Authors: Zhang Qianyu , Wang Tong , Jin Jiawei , Shi Xiaoqian , Ma Zhenru , Ma Runlin Z. , Fang Qiuhong 
Published: April 2020

Abstract
Background: Chronic obstructive pulmonary disease (COPD) is a highly prevalent lung disease worldwide and imposes increasing disease burdens globally. COPD is characterized by irreversible airflow obstruction. Emphysema is one of the primary pathological features causing the irreversible decline of pulmonary function, while the precise mechanisms behind emphysema remain unclear. Reticulocalbin 3 (Rcn3) is an endoplasmic reticulum (ER) lumen protein localized in the secretory pathway of living cells. We have reported that Rcn3 in type II alveolar epithelial cell (AECIIs) plays a critical role in perinatal lung development and bleomycin-induced lung injury-repair. Since is associated with alveolar epithelial disruption, Rcn3 might be involved in the development of emphysema during COPD. Materials and Methods : We examined Rcn3 expression in lung specimens from patients (44 COPD patients and 26 non-COPD control patients) undergoing lung lobectomy or pneumonectomy. Mouse models of COPD and emphysema were established by cigarette smoke (CS) exposure and intratracheal installation of pancreatic porcine elastase (PPE), respectively. Rcn3 expression was detected in the lung tissues from these mice. Furthermore, conditional knockout (CKO) mice with Rcn3 deletion specific to AECIIs, were used to explore the role of Rcn3 in PPE-induced emphysema progression. Rcn3 protein expression in lung tissues were evaluated by Western blot and immunohistochemistry. Rcn3 mRNA expression in lung tissues was detected by qPCR.Results : Compared with non-COPD patients, Rcn3 expression was significantly increased in the lung specimens from COPD patients. Rcn3 expression was also significantly up-regulated in the lung tissues from COPD mice and emphysematous mice. Moreover, selective ablation of Rcn3 in AEC IIs significantly alleviated severity of the mouse emphysema in response to intratracheal installation of PPE.Conclusions: Our data, for the first time, indicated that Rcn3 in AECIIs might play a role in modulating the progression of emphysema, and thus be involved in the development of COPD. Suppression of Rcn3 expression in AECIIs may have a beneficial effect on COPD.This work was supported by National Natural Science Foundation of China (No. 81641004).
Altered expression of IL-18, IL-18 binding protein and IL-18 receptor in blood monocytes of patients with allergic rhinitis
Authors: Wang Junling , Gu Fangqiu , Li Zhi , Zhan Mengmeng , Wang Ling , Zhang Huiyun , Chen Dong , Xie Hua , Chai Ruonan , Zhao Nan , Yang Ruiming , Hu Yalin , He Shaoheng 
Published: April 2020

Abstract
Background: Interleukin (IL)-18 is emerging as an attractive participant in allergic rhinitis (AR). However, correlation of IL-18 with IL-18 binding protein (BP) in plasma, and expression of IL-18, IL-18BP and IL-18 receptor (R) in AR blood monocytes remain obscure.Methods: We investigated IL-18, IL-18BP and IL-18R expression in monocytes by using flow cytometric analysis, murine AR model, and quantitative real-time PCR in the present study.Results: It was found that plasma IL-18 and IL-1β in AR patients was higher than those in healthy control subjects. Free (f)IL-18 had a high correlation with IL-18BP, IL-1β and TNF-α in AR plasma. Proportion of IL-18 + monocytes was increased, whereas IL-18BP + monocytes were decreased in blood of patients with AR. It was found that Platanus pollen allergen extract provoked the elevated expression of IL-18 and IL-18R in AR blood monocytes. Dermatophagoides pteronyssinus , Artemisia sieversiana wild and Platanus pollen allergen extracts enhanced IL-18R protein and mRNA expression in primary monocytes from AR patients. Moreover, numbers of macrophages and IL-18R + macrophages in nasal lavage fluid (NLF) were increased, and levels of IL-18 in both plasma and NLF were elevated in AR mice.Conclusions: IL-18 is likely to participate in the development of AR as a causative factor, therefore, it could be a therapeutic target for AR.
Real-time Monitoring of Bacterial Biofilms Metabolic Activity by a Redox-Reactive Nanosensors Array
Authors: Davidi Ella Yeor , Zverzhinetsky Marina , Krivitsky Vadim , Patolsky Fernando 
Published: April 2020

Abstract
Background: Bacterial biofilms are communities of surface-associated microorganisms living in cellular clusters or micro-colonies, encapsulated in a complex matrix composed of an extracellular polymeric substance, separated by open water channels that act as a circulatory system that enable better diffusion of nutrients and easier removal of metabolic waste products. The monitoring of biofilms can provide important information on fundamental biofilm-related processes. That information can shed light on the bacterial processes and enable scientists to find ways of preventing future bacterial infections. Various approaches in use for biofilm analysis are based on microscopic, spectrochemical, electrochemical and piezoelectrical methods. All these methods provide significant progress in understanding the bio-process related to biofilm formation and eradication, nevertheless, the development of novel approaches for the real-time monitoring of biochemical, in particular metabolic activity, of bacterial species during the formation, life and eradication of biofilms is of great potential importance.Results: Here, detection and monitoring of the metabolic activity of bacterial biofilms in high-ionic-strength solutions were enabled as a result of novel surface modification by an active redox system, composed of 9,10-dihydroxyanthracene/9,10-anthraquinone, on the oxide layer of the SiNW, yielding a chemically-gated FET array. With the use of enzymatic reactions of oxidases, metabolites can be converted to and monitored by the nanosensors. Here, the successful detection of glucose consumption in high-ionic-strength solutions, such as bacterial media, without pre-processing of small volume samples under different conditions and treatments, has been demonstrated. The biofilms were treated with antibiotics differing in their mechanisms of action and were compared to untreated biofilms. Further examination of biofilms under antibiotic treatment with SiNW-FET devices could shed light on the bioprocess that occurs within the biofilm. Moreover, finding a proper treatment that eliminates the biofilm could be examined by the novel nanosensor array as a monitoring tool.Conclusions: To summarize, the combination of redox-reactive SiNW-FET devices with microfluidic techniques enables the performance of label-free, rapid, automated, multiplex and real-time noninvasive metabolites detection for the investigation of bacterial biofilms. This novel platform can be used as an extremely sensitive tool for detection and establishing medical solutions for bacterial-biofilm eradication and for finding a proper treatment to eliminate biofilm contaminations. Moreover, the sensing system can be used as a research tool for further understanding of the metabolic processes that occur within the bacterial biofilm population.
Stage IIIA pathologic N2 non-small cell lung cancer patients may maximize benefit from preoperative radiotherapy: a population-based study
Authors: Duan Hongxia , Liang Long , Xie Shuanshuan , Wang Changhui 
Published: April 2020

Abstract
Purpose: The aim of this study was to investigate the best surgery and radiotherapy sequence in patients affected by stage IIIA pathologic N2 (IIIA/N2) non-small-cell lung cancer (NSCLC) and to identify its potential risk factors.Method: A total of 17,654 patients (8,786 men and 8,868 women), diagnosed with NSCLC stage IIIA-N2 from 2004 to 2015 were identified in the Surveillance, Epidemiology, and End Results (SEER) database. Among the relevant clinical parameters, we evaluated overall survival (OS), lung cancer-specific survival (LCSS) and other variables such as age, sex and tumor size in patients who were treated with different combinations of surgery and radiotherapy strategies.Results: We discovered that among patients who refused radiotherapy, there was a higher prevalence of elderly individuals and patients who underwent less chemotherapy than the average. Survival of patients treated by radiotherapy combined with surgery was higher than that of patients who underwent surgery only (p < 0.001). Compared with surgery only, preoperative radiotherapy significantly improved the survival rate most (p < 0.001). When we performed the OS and LCSS analysis in the subgroup of patients who were >75 years old, who underwent postoperative radiotherapy (PORT) had the highest survival rate in all groups (p < 0.001). Multivariate analyses showed that the following parameters had a negative impact on survival: female sex, older age, no chemotherapy, large tumor size, high tumor grade, no surgery or radiotherapy.Conclusions: In IIIA/N2 NSCLC patients, the use of radiotherapy and chemotherapy resulted in better survival, while preoperative radiotherapy is the most promising regimen, except for patients whose age > 75.
Patterns of food parenting practices regarding junk food and sugary drinks among parent-child dyads
Authors: Thomson Jessica , Hennessy Erin , Landry Alicia , Goodman Melissa 
Published: April 2020

Abstract
Background: Children’s food preference and intake patterns are affected by parental child feeding practices. The objective of this study was to determine patterns of food parenting practices regarding junk food and sugary drinks (JS) and to investigate their associations with demographic characteristics and dietary intake in a large cohort of parents and their children (12-17 years). Methods: Dyadic survey data from the cross-sectional, internet-based Family Life, Activity, Sun, Health, and Eating Study, conducted in 2014, were analyzed using latent class analysis to identify patterns of parent- and child-reported JS parenting practices (n=6). Model covariates included self-reported parent sex, body mass index, sugar intake, and legitimacy of parental authority; and self-reported child age, sex, body mass index percentile, sugar intake, and legitimacy of parental authority. Results: Based on 1,657 parent-child dyads, five latent classes were identified – Complete Influencers (28%), Indifferent Influencers (21%), Negative Influencers (20%), Minimal Influencers (18%), and Disagreeing Influencers (13%). Compared to older child dyads, younger child dyads had 77% and 65% lower odds of belonging to Indifferent and Minimal Influencers versus Complete Influencers. Odds of belonging to Negative Influencers were 3% higher for every teaspoon increase in parent sugar intake from sugar-sweetened beverages while odds for Minimal Influencers were 8% lower for every teaspoon increase in child sugar intake from sugar-sweetened beverages versus Complete Influencers. Dyads with low compared to high parental and child agreement with legitimacy of parental authority regarding JS had between 2 and 27 times the odds of belonging to one of the other classes versus Complete Influencers. Conclusions: The study findings suggest that parents utilize distinct patterns of feeding practices related to JS, and those patterns vary by dyadic demographic characteristics, dietary intake, and perceived legitimacy of parental authority. Counseling or intervening with parents to use a mix of structure practices to positively influence their child’s and possibly their own intake of sugary snacks and drinks may prove more efficacious than use of coercive control practices.
Looking for Painting Mixtures to Glimpse Pictorial Techniques: A Micro-Stratigraphic Physicochemical Approach to the Rock Art From the Oyola’s Caves (Argentina)
Authors: Gheco Lucas Ignacio , Marcos Tascon , Eugenia Ahets Etcheberry , Quesada Marcos Nicolás , Fernando Marte 
Published: April 2020

Abstract
Could not be possible that rock paintings with similar hues and morphologies were the results of different painting events inside a cave but distanced in time? Is there any archaeometric approach that allows us to evidence these subtle differences? Aiming to address these inquires, in this work are presented the potentials of new physicochemical lines of evidence for characterization and differentiation of painting mixtures. This will improve the understanding of the technical heterogeneity and temporal complexity of painting sets executed in a particular archaeological site. In order to explore these points, the results obtained in the micro-stratigraphic studies of samples taken from the painted walls of Oyola, an archaeological site situated in the northwest of Argentina, are presented. These samples have been analyzed by micro-Raman spectroscopy (mRS) and scanning electron microscopy with energy dispersive X-ray (SEM-EDS). The differences found by cross-section chemical studies could be explained as a consequence of two situations: 1- differences in the painting’s chemical components, either in the pigments involved or additives used; and, 2- differences in the physical properties of the mixtures such as stratum thickness and/or particle size. Also, in this article is explained each of the hypothesis, presenting the limits of these interpretations and pointing out future research challenges. As we discussed, it could be possible that the chemical and physical differences found between painting mixtures were the material expression of varied types of pictorial techniques.
Comparison of a flexible versus rigid bone cement injection system in unilateral percutaneous vertebroplasty
Authors: li yuwei , cui wei , zhou peng , li cheng , wen yan , xiao wei 
Published: April 2020

Abstract
Background: Percutaneous vertebroplasty (PVP) has been demonstrated to be effective in the treatment of acute osteoporotic vertebral fracture (AOVF). However, bilateral puncture takes more time to accept more X-ray irradiation; some scholars apply unilateral puncture PVP, but the cement cannot be symmetrically distributed in the vertebral body, so we use a flexible cement injector that undergoes PVP through the unilateral pedicle puncture. This research aims to compare the clinical results of PVP for AOVF with unilateral pedicle puncture using a straight bone cement injector and a bendable cement injector, determine the value of a bendable cement injector. Methods: We undertook a retrospective analysis of patients with thoracic and lumbar compression fracture treated with unilateral pedicle puncture Percutaneous vertebroplasty from our institution from June 2013 to July 2015. Operation time, radiation exposure, bone cement injection amount, and the incidence of bone cement leakage were recorded on presentation, the cement leakage was measured by x-ray and computed tomography scan. The patients were followed up postoperatively and were assessed mainly with regard to clinical and radiological outcomes. Results: There was no significant difference in the operation time, radiation exposure time and incidence of bone cement leakage between the two groups. There was significant difference in the amount of bone cement injection and the difference between the two groups. there were no significant differences in VAS and the relative height of the vertebral body and local Cobb angle and QUALEFFO between 2 groups at 1 week after PVP, only significant difference was observed between 12 months after operation. Conclusions: Application of flexible cement injector is safe and feasible,Compared with the application of straight bone cement injector, without prolonging the operative time, radiation exposure time and the incidence of bone cement leakage, it has the advantages of good long-term effect and low incidence of vertebral fracture recurrence.
COVID-19 presented with Deep Vein Thrombosis: An unusual case report
Authors: Davoodi Lotfollah , Taghavi Morteza , Razavi Alireza 
Published: April 2020

Abstract
BackgroundOn 31 December 2019, the World Health Organization (WHO) was informed of a cluster of cases of pneumonia of unknown cause detected in Wuhan City, Hubei Province, China. The pneumonia was caused by a virus called SARS-Cov-2, which was later named COVID-19. In this report, we present a patient with COVID-19 who developed deep vein thrombosis.Case presentationA 57-year-old woman presented to the clinic's infectious department with no underlying illness due to pain, redness, and leg swelling. According to a patient report, she had a mild dry cough for the past 3 days and had no other symptoms. The patient had no history of prone thrombosis conditions. Initially, CT angiography was performed to rule out pulmonary thromboembolism, which showed no evidence of thrombosis. Dilatation and thrombosis were seen in the examinations of the paired veins of the leg, popliteal, superficial and left femoral joints, and no evidence of vascular flow suggesting acute DVT. Because of fever and lymphopenia, nasal swabs were used for sampling and SARS-CoV-2 nucleic acid was detected by RT-PCR. Chest X-ray also revealed bilateral patchy ground-glass opacity. Other tests including ANA, Anti-dsDNA, RF test and ACA test was normal. Heparin at a dose of 80 units/kg IV bolus, chloroquine 400 mg single dose and lopinavir/ritonavir (Kaletra) 400 mg twice daily were prescribed to treat illness and relieve symptoms. On illness day 3, fever stopped and nasal swab sample turned undetectable for SARS-CoV-2 by RT-PCR as well as swelling and tenderness on her leg had been disappeared gradually. She is under regular follow-up with no new symptoms to date.Conclusion The mechanism of DVT formation due to COVID-19 is unknown despite thrombocytopenia, and has not been investigated but it resolved as COVID-19 symptoms, tenderness, and leg pain improved. Although COVID-19 presented with Deep Vein Thrombosis is a rare condition, in middle-aged people with sudden onset of manifestations, we should recognize it from other diseases as an important and treatable differential diagnosis. Rapid diagnostic assays, efficient treatment, and prudent use of CT-scan are important to control future COVID-19 spread.
Presentation and validation of induction irrigation as an efficient and profitable method
Authors: Bozorg-Haddad Omid , Bahrami Mahdi , Loáiciga Hugo A. 
Published: April 2020

Abstract
The scarcity of water resources along with population growth and low precipitation exert stresses on the agricultural sectors of arid regions. The evapotranspiration losses due to water use in the warm months of the year are high for walnut production, for example, which is one of the main Iranian agricultural exports. Also, the water losses occurring in flood irrigation, surface storage, and water conveyance, and the high costs of drip irrigation increase the need for efficient irrigation methods. This study proposes induction irrigation as a novel method for preventing evapotranspiration losses, increasing water-use efficiency, and raising agricultural profitability. Induction irrigation relies on recharge and extraction wells to inject treated sewage and withdraw groundwater, respectively, and create a saturated zone in the plants' root area. This paper demonstrates that induction irrigation applied to walnut orchards would lower costs and water losses, avoid surface pollution, and increase agricultural profitability.
Potential for bifenthrin removal using microalgae from a natural source
Authors: Weis Leticia , de Cassia de Souza Schneider Rosana , Hoeltz Michele , Rieger Alexandre , Tostes Schirley , Lobo Eduardo A. 
Published: April 2020

Abstract
The accumulation of environmental pesticides can cause problems in aquatic ecosystems and adverse effects in humans. These compounds can be found in water due to runoff from agricultural, industrial and domestic applications. In Southern Brazil, tobacco cultivation is one of the most important economic agricultural activities. The bifenthrin pesticide, classified as having moderate toxicity (class II), is commonly used as an insecticide in this culture. In this context, the present research aimed to study the performance of microalgae-induced bioremediation processes. Microalgae were isolated from a natural water source in the city of Santa Cruz do Sul, RS, Brazil, which is an artificial reservoir used for public water supply. For this purpose, biodegradation, biosorption, influence of pH, percentage of inoculum and photoperiod were evaluated in batch experiments for 20 cultivation days. After the phycoremediation process, the bifenthrin pesticide (m/z = 181) was quantified by gas chromatography with mass spectrometry (GC-MS). The results indicated that microalgae isolated from the water of the lake were able to contribute to the removal of approximately 99% of bifenthrin through biodegradation and biosorption processes. Photodegradation was identified (&gt;77%) and the best condition for the phycoremediation was 20% inoculum with a photoperiod of 18:6 h.