bluuebunny/crossref_metadata_2025_split huggingface dataset:
A research of STEAP1 regulated gastric cancer cell proliferation, migration and invasion in vitro and in vivos
Authors: Zhang Zhe , Hou Wen‐bin , Zhang Chao , Tan Yu‐en , Zhang Dong‐dong , An Wen , Pan Si‐wei , Wu Wan‐di , Chen Qing‐chuan , Xu Hui‐mian 
Published: October 2020

AbstractSix‐Transmembrane Epithelial Antigene of the Prostate 1 (STEAP1) is associated with the occurrence and development of cancer. This study aimed to clarify the role of STEAP1 in gastric cancer tumour growth and metastasis, as well as its molecular mechanism of action.Statistical methods were used for clinical data analysis. Protein expression was detected using immunohistochemistry(IHC). The mRNA and protein expression in the cell cultures were detected using reverse transcription‐polymerase chain reaction(RT‐PCR) and western blot analysis. Overexpression and silencing models were constructed using plasmid and lentivirus transfection. To detect cell proliferation in vitro, Cell Counting Kit‐8(CCK‐8), flow cytometry and colony formation assays were used; transwell and wound healing assays were used to detect cell migration and invasion;For in vivo experiments, nude BALB/c mice were used for detecting subcutaneous tumorigenesis and intraperitoneal implantation. In the results,we found STEAP1 was overexpressed in gastric cancer tissues and cell lines. Single‐factor and Cox analyses showed that STEAP1 gene expression level correlated with poor prognosis. Up‐regulation of STEAP1 increased cell proliferation, migration and invasion, which decreased after STEAP1 was knocked down. These changes were achieved via the activation of the AKT/FoxO1 pathway and epithelial‐mesenchymal transformation (EMT). The in vivo animal experiments showed that STEAP1 knock down, resulted in a decrease in the subcutaneous tumour and peritoneal tumour formation.
Molecular docking‐assisted screening reveals tannic acid as a natural protein disulphide isomerase inhibitor with antiplatelet and antithrombotic activities
Authors: Ren Lijie , You Tao , Li Qing , Chen Guona , Liu Ziting , Zhao Xuefei , Wang Yinyan , Wang Lei , Wu Yi , Tang Chaojun , Zhu Li 
Published: October 2020

AbstractProtein disulphide isomerase (PDI) promotes platelet activation and constitutes a novel antithrombotic target. In this study, we reported that a PDI‐binding plant polyphenol, tannic acid (TA), inhibits PDI activity, platelet activation and thrombus formation. Molecular docking using plant polyphenols from dietary sources with cardiovascular benefits revealed TA as the most potent binding molecule with PDI active centre. Surface plasmon resonance demonstrated that TA bound PDI with high affinity. Using Di‐eosin‐glutathione disulphide fluorescence assay and PDI assay kit, we showed that TA inhibited PDI activity. In isolated platelets, TA inhibited platelet aggregation stimulated by either GPVI or ITAM pathway agonists. Flow cytometry showed that TA inhibited thrombin‐ or CRP‐stimulated platelet activation, as reflected by reduced granule secretion and integrin activation. TA also reduced platelet spreading on immobilized fibrinogen and platelet adhesion under flow conditions. In a laser‐induced vascular injury mouse model, intraperitoneal injection of TA significantly decreased the size of cremaster arteriole thrombi. No prolongation of mouse jugular vein and tail‐bleeding time was observed after TA administration. Therefore, we identified TA from natural polyphenols as a novel inhibitor of PDI function. TA inhibits platelet activation and thrombus formation, suggesting it as a potential antithrombotic agent.
Female mating experience and genetic background independently influence male mating success in fruit flies
Authors: Filice David C. S. , Bhargava Rajat , Dukas Reuven 
Published: October 2020

AbstractWhen the reproductive interests of males and females conflict, males can evolve traits that are harmful to females, and females can coevolve traits to resist this harm. In the fruit fly, Drosophila melanogaster, there is genetic variation in female resistance traits, which can affect the pre‐ and post‐mating success of males that try to mate with them. However, it is not clear to what extent the expression of these phenotypes can be modified by environmental factors such as sociosexual experience. Here, we tested how the genetic background of a female and her previous mating experience interact to affect the mating success of focal males. In the experience phase, we placed females from 28 distinct genetic backgrounds individually either with a single male (low conflict) or with three males (high conflict) for 48 hr. In the subsequent test phase, we measured the mating and post‐mating fertilization success of focal males paired individually with each female. We found that focal males paired with females from the high‐conflict treatment were less successful at mating, took longer to mate when they were successful, and had a lower proportion of paternity share. Furthermore, we identified significant female genetic variation associated with male mating success. These results indicate that female experience, along with intrinsic genetic factors, can independently influence different fitness components of her subsequent mates and has implications for our understanding of plastic female mating strategies and the evolution of sexually antagonistic traits in males and females.
Sequential valorization strategies for dairy wastewater and water hyacinth to produce fuel and fertilizer
Authors: Arutselvy Balakrishnan , Rajeswari Gunasekaran , Jacob Samuel 
Published: October 2020

AbstractTo overcome the obstacles of treatment of dairy wastewater (DW) under anaerobic mono‐digestion and sustainable disposal of harvested water hyacinth (WH) a novel sequential valorization strategy was adopted to obtain fuel and fertilizer. Initially, DW and WH were subjected for anaerobic co‐digestion to elucidate the synergistic effect of the substrates to improve the process efficiency and increased biomethane production. The results indicated that the co‐digestion mixture (DW: WH‐1:1) has resulted in biomethane yield of 504.38 L/Kg VSfed which is 1.28‐fold and 3.14‐fold higher as compared to the mono‐digestion of WH (431.71 L/Kg VSfed) and DW (160.59 L/Kg VSfed), respectively. Further, the residual digestate was subjected for slow pyrolysis (320 ± 15°C) to obtain bio‐oil and biochar. The obtained bio‐oil shown superior fuel characteristics as compared to direct WH bio‐oil resulting in density, viscosity, and calorific value of 1.21 ± 0.04 g/ml, 1.8 ± 0.05 cSt and 1766.82 ± 11.53 KJ/Kg, respectively. The biochar residue from pyrolysis was amended to the soil to determine its bio‐fertilizing property. The soil properties in terms of bulk density, porosity, organic carbon, and pH were enhanced. Tomato cultivation in biochar amended soil shown comparable vegetative growth to that of digestate and urea thereby advocating the replacement of chemical fertilizers.Practical ApplicationWith recent increase in environmental concerns and requirement to meet the prescribed limits for effluent discharge from the industries, especially from food processing for the protection of aquatic bodies, there is a requirement of adoption of cost effective technology that could provide both economic and ecological sustainability. In this study, a sequential valorization strategy was adopted for efficient management of dairy wastewater and harvested water hyacinth from the water resources. The resultant products biogas, bio‐oil and biochar could be potentially used as fuel and fertilizer. Circular bio‐economy is in need of time which could be attainable by the implementation of such strategies in large scale industries for efficient waste management and resource recovery. This will not only benefit the environment but also to the society by galvanizing the sustainable development technology.
Association of Hospitalization with Long‐Term Cognitive Trajectories in Older Adults
Authors: Sprung Juraj , Knopman David S. , Petersen Ronald C. , Mielke Michelle M. , Weingarten Toby N. , Vassilaki Maria , Martin David P. , Schulte Phillip J. , Hanson Andrew C. , Schroeder Darrell R. , Laporta Mariana L. , White Robert J. , Vemuri Prashanthi , Warner David O. 
Published: October 2020

IMPORTANCEHospitalizations are associated with cognitive decline in older adults.OBJECTIVETo determine the association between hospitalization characteristics and the trajectory of cognitive function in older adults.DESIGNPopulation‐based longitudinal study of cognitive aging.SETTINGOlmsted Medical Center and Mayo Clinic, the only centers in Olmsted County, Minnesota, with hospitalization capacity.PARTICIPANTSIndividuals without dementia at baseline, with consecutive cognitive assessments from 2004 through 2017, and at least one visit after the age of 60.MEASUREMENTSThe primary outcome was longitudinal changes in global cognitive z‐score. Secondary outcomes were changes in four cognitive domains: memory, attention/executive function, language, and visuospatial skills. Hospitalization characteristics analyzed included elective versus nonelective, medical versus surgical, critical care versus no critical care admission, and long versus short duration admissions.RESULTSOf 4,587 participants, 1,622 had 1 and more hospital admission. Before hospitalization, the average slope of the global z‐score was −0.031 units/year. After hospitalization, the rate of annual global z‐score accelerated by −0.051 (95% CI = −0.057, −0.045) units, P < .001, resulting in an estimated annual slope after the first hospitalization of −0.082. The accelerated decline was found in all four cognitive domains (memory, visuospatial, language, and executive, all P < .001). The acceleration of the decline in global z‐score following hospitalization was greater for medical compared to surgical hospitalizations (slope change following hospitalization = −0.064 vs −0.034 for medical vs surgical, P < .001), and nonelective compared to elective admissions (slope change following hospitalization = −0.075 vs −0.037 for nonelective vs elective, P < .001). The acceleration of cognitive decline was not different for hospitalization with intensive care unit admission versus not.CONCLUSIONSHospitalization of older adults is associated with accelerated decline of global and domain‐specific cognitive domains, with the rate of decline dependent upon type of admission. The clinical impact of this accelerated decline will depend on the individual's baseline cognitive reserve and expected longevity.
Imagining Brazil in Africa: Capoeira's Transatlantic Roots and Routes
Authors: Wesolowski Katya 
Published: October 2020

ResumenEste artigo examina cidades africanas como os mais recentes pontos do circuito transnacional da capoeira. Uma África imaginada tem sido parte integrante da prática da capoeira no Brasil, porém, apenas recentemente foi que capoeiristas brasileiros começaram a viajar à África. Baseado em trabalho etnográfico realizado no Brasil e em Angola, examino esse “retorno” da capoeira à sua pátria imaginada e argumento que esse fluxo no Sul Global é um processo recíproco de imaginação: enquanto os capoeiristas brasileiros imaginam que Angola possui as raízes geradoras de sua prática, os capoeiristas angolanos imaginam o Brasil como o preservador dessas raízes. Os jovens de Luanda e Benguela abraçam a prática como uma cura para a crise de identidade comunitária resultante de quase meio século de guerra. Tanto os capoeiristas angolanos quanto os brasileiros mobilizam a capoeira para se enraizarem em um passado local e, ao mesmo tempo, criarem um futuro global de mobilidade transnacional e cosmopolitismo. [Brasil, Angola, diáspora, capoeira, cultura expressiva]
Wavefront‐sensorless adaptive optics with a laser‐free spinning disk confocal microscope
Authors: Hussain Syed Asad , Kubo Toshiki , Hall Nicholas , Gala Dalia , Hampson Karen , Parton Richard , Phillips Mick A. , Wincott Matthew , Fujita Katsumasa , Davis Ilan , Dobbie Ian , Booth Martin J. 
Published: October 2020

AbstractAdaptive optics is being applied widely to a range of microscopies in order to improve imaging quality in the presence of specimen‐induced aberrations. We present here the first implementation of wavefront‐sensorless adaptive optics for a laser‐free, aperture correlation, spinning disk microscope. This widefield method provides confocal‐like optical sectioning through use of a patterned disk in the illumination and detection paths. Like other high‐resolution microscopes, its operation is compromised by aberrations due to refractive index mismatch and variations within the specimen. Correction of such aberrations shows improved signal level, contrast and resolution.
The use of radiofrequency‐assisted lipolysis with radiofrequency microneedling in premature jowl and neck laxity following facialplasty
Authors: Demesh Daniel , Cristel Robert T. , Gandhi Nimit D. , Kola Eljona , Dayan Steven H. 
Published: October 2020

AbstractBackgroundA subset of facelift patients have premature redevelopment of skin laxity in the lower face and neck. Many patients seek alternatives to revision facelifts to avoid high risks and costs. Radiofrequency‐assisted lipolysis (RFAL) with Radiofrequency (RF) microneedling may be alternative minimally invasive options.Objective/AimTo evaluate the efficacy of radiofrequency energy devices for treatment of premature jowl and neck skin laxity following facialplasty.MethodsThis is a single‐center, prospective study of patients seeking treatment for jowl and neck skin laxity 1‐5 years following facialplasty. Treatment was performed with the InMode radiofrequency AccuTite® and Morpheus8® systems. Study duration was 12 months with 6 months of follow‐up. Endpoints included improvement in skin tightening assessed by blinded investigators, and investigator and subject assessment of skin appearance. Subjects also rated satisfaction with treatment and pain levels.ResultsThe study protocol was completed by nine patients. Based on investigator evaluations, 33% had marked improvement at 3 months, which increased to 55% at 6‐month postprocedure. Patient‐reported improvement was “markedly improved” in 67%, “moderate improvement” in 11%, and “slight improvement” in 22% at 3 months. Overall patient satisfaction was rated as “very satisfied” by 33% and “satisfied” by 67% at 3 months. There were no adverse events reported.ConclusionThe results of this study provide supporting evidence that RFAL technology can provide a safe, minimally invasive, and effective treatment for skin laxity in the jowls and neck in patients who desire further correction after undergoing primary facelift.
Glycerine as a feed supplement for beef and dairy cattle: A meta‐analysis on performance, rumen fermentation, blood metabolites and product characteristics
Authors: Syahniar Theo M. , Andriani Mira , Ridla Muhammad , Laconi Erika B. , Nahrowi Nahrowi , Jayanegara Anuraga 
Published: October 2020

AbstractThis study aimed to comprehensively evaluate the effects of glycerine supplementation at various concentrations on performance, rumen fermentation, blood metabolites and product characteristics of beef and dairy cattle in vivo by using a quantitative meta‐analysis approach. Meta‐analysis was performed by integrating a total of 52 studies from 39 articles and 182 treatments into a database. Data were constructed into an intact database and did not distinguish between beef and dairy cattle, except for the parameters of production performance and product characteristics. Data summarized were analysed by using a statistical meta‐analysis that employed a fixed effect of glycerine supplementation level and a random effect of various studies for both beef and dairy cattle. Significance of an effect was stated at the probability level of p < .05, and p < .1 was considered as a tendency of significant. Results revealed that there was a linear decrease on dry matter intake (p < .01) and daily gain (p < .05) of beef cattle with the increasing levels of glycerine supplementation. Glycerine supplementation did not decrease milk production of lactating dairy cows. Molar proportion of acetate in the rumen was decreased (p < .001), whereas propionate and butyrate proportions were increased (both at p < .001) by glycerine supplementation. Generally, glycerine did not change nutrient digestibility except that it reduced fibre digestibility (p < .001). Glycerine supplementation linearly lowered triglyceride and NEFA concentrations (both at p < .05) in the blood serum, but not other blood metabolites. Glycerine tended to linearly increase (p < .1) carcass percentage in beef cattle. Increasing dietary glycerine levels decreased milk fat (p < .01) but elevated milk protein (p < .001). Glycerine tended to increase milk lactose (p < .1) by following a quadratic pattern. The proportion of MUFA was increased quadratically by glycerine supplementation (p < .05), whereas glycerine tended to decrease SFA by following a quadratic pattern (p < .1).
New Eocene fossil fruits and leaves of Menispermaceae from the central Tibetan Plateau and their biogeographic implications
Authors: Del Rio Cédric , Huang Jian , Liu Ping , Deng Wei‐Yu‐Dong , Spicer Teresa E.V. , Wu Fei‐Xiang , Zhou Zhe‐Kun , Su Tao 
Published: October 2020

AbstractMenispermaceae are a pantropical and temperate family with an extensive fossil record during the Paleogene period, especially in North America and Europe, but with much less evidence from Asia. The latest fossil evidence indicates a succession of tropical to subtropical flora on the central Tibetan Plateau during the Paleogene. However, the biogeographic histories of these floras are still unresolved. Here, we report on endocarps and leaves of Menispermaceae from the Middle Eocene of Jianglang village, Bangor County, central Tibetan Plateau. The endocarps belong to two genera:Stephania, which is characterized by a horseshoe‐shaped endocarp and with one lateral crest ornamented by spiny to rectangular ribs, and a condyle area; andCissampelos(s.l.), which has two characteristic lateral ridges and a conspicuous external condyle. Associated leaves belong to the genusMenispermites, and are characterized by actinodromous primary venation, brochidodromous secondary veins, entire margins, and the presence of marginal secondary veins. The biogeographic history of Menispermaceae is complex, but evidence from these new fossils indicates an early diversification of the group in Asia, probably in response to the warming climate during the Eocene. The Jianglang flora appears to be part of a boreotropical flora, connecting Asia with North American and European floras during the Middle Eocene. The modern distribution of menispermaceous taxa found in Jianglang, as well as other families represented in the Jianglang flora, show that a tropical to subtropical climate occurred during the Eocene in central Tibet.
Seizures in dogs under primary veterinary care in the United Kingdom: Etiology, diagnostic testing, and clinical management
Authors: Erlen Alexander , Potschka Heidrun , Volk Holger A. , Sauter‐Louis Carola , O'Neill Dan G. 
Published: October 2020

AbstractBackgroundAlthough seizures are common in dogs, limited published information is available on the classifications of seizures, diagnostic approaches, or clinical management of seizure‐affected patients in the veterinary primary care setting.ObjectivesExplore seizure etiology, diagnostic testing, and clinical management of seizure‐affected dogs in the primary care veterinary setting.AnimalsA total of 455 553 dogs in VetCompass.MethodsCross‐sectional analysis by cohort clinical data.ResultsFrom 2834 incident seizure cases, we identified 579 (20.5%) dogs with epilepsy based on the International Veterinary Epilepsy Task Force (IVETF) classification system, including 484 (17.1%) with idiopathic epilepsy, 95 (3.4%) with structural epilepsy, and 179 dogs (6.3%) with reactive seizures. In their clinical first opinion records, 245 (8.6%) cases were recorded with epilepsy. Overall, 1415 (49.9%) cases received diagnostic evaluation equivalent to or higher than IVETF Tier 1 diagnostic testing.Being <12 years of age and being insured were risk factors for receiving IVETF Tier 1 or higher diagnostic evaluation among seizure cases. Anti‐seizure drug (ASD) treatment was not prescribed for 1960/2834 (69.2%) dogs in association with the incident seizure event. Of the remainder, 719 (25.3%) dogs received 1 ASD, whereas 155 (5.5%) an ASD combination.Conclusion and clinical importanceThe differences between seizure classifications in the clinical records and those retrospectively assigned by the researchers support the need for clearer diagnostic guidelines in clinical practice. Insured dogs and dogs <12 years of age were more likely to receive advanced diagnostic evaluation, suggesting that financial and perceived prognostic factors influence case management.
Effects of calcifediol supplementation on markers of chronic kidney disease‐mineral and bone disorder in dogs with chronic kidney disease
Authors: Parker Valerie J. , Rudinsky Adam J. , Benedict Jason A. , Beizaei Azadeh , Chew Dennis J. 
Published: October 2020

AbstractBackgroundChronic kidney disease‐mineral and bone disorder (CKD‐MBD) in dogs is associated with hypovitaminosis D, increased parathyroid hormone (PTH), and increased fibroblast growth factor‐23 (FGF‐23) concentrations. Best practice for vitamin D metabolite supplementation in CKD‐MBD remains unknown.ObjectiveTo provide an extended‐release calcifediol supplement to dogs with CKD and to measure its effects on variables indicative of CKD‐MBD.AnimalsTen dogs with International Renal Interest Society stages 2 and 3 CKD.MethodsIn a prospective study, dogs received a calcifediol supplement for 84 days. Serum 25‐hydroxyvitamin D (25[OH]D), 1,25‐dihydroxyvitamin D (1,25[OH]2D), 24,25‐dihydroxyvitamin D (24,25[OH]2D), creatinine, calcium, phosphorus, PTH, plasma FGF‐23 concentrations, and urine profiles were measured monthly during supplementation. Urine calcium to creatinine (UCa/Cr) ratios and fractional excretion of calcium, phosphorus, and sodium were determined.ResultsAll serum vitamin D metabolite concentrations increased significantly by day 84 (P < .001): [25(OH)D (median 249.9 ng/mL; range, 149.7‐469.9 ng/mL) compared to baseline (median 50.2 ng/mL; range, 31.3‐66.0 ng/mL); 1,25(OH)2D (median 66.1 pg/mL; range, 56.9‐88.1 pg/mL) compared to baseline (median 37.3 pg/mL; range, 29.3‐56.7 pg/mL); 24,25(OH)2D (median 81.4 ng/mL; range, 22.1‐151.7 ng/mL) compared to baseline (median 15.4 ng/mL; range, 6.9‐40.6 ng/mL)]. There were no significant differences in calcium, phosphorus, PTH concentrations, UCa/Cr or fractional excretion of calcium. No dog developed ionized hypercalcemia. Plasma FGF‐23 concentrations increased by day 84 (median 1219 pg/mL; range, 229‐8824 pg/mL) compared to baseline (median 798 pg/mL; range, 103‐4.145 pg/mL) (P < .01).Conclusions and Clinical ImportanceCalcifediol supplementation for 84 days was well‐tolerated in dogs with IRIS stages 2 and 3 CKD. It remains to be determined how long‐term supplementation would affect CKD progression and QOL.