bluuebunny/crossref_metadata_2025_split huggingface dataset:
Epidemiologic, Clinical, and Laboratory Findings of the COVID-19 in the current pandemic: Systematic Review and Meta-analysis
Authors: Xie Yewei , Wang Zaisheng , Liao Huipeng , Marley Gifty , Wu Dan , Tang Weiming 
Published: August 2020

Abstract
Background: The COVID-19 pandemic has affected the world deeply, with more than 3,000,000 people infected and nearly 200,000 deaths. This review aimed to summarize the epidemiologic traits, clinical spectrum, CT results and laboratory findings of the COVID-19 pandemic.Methods: We scoped for relevant literatures published during 1st Dec 2019 to 23rd Apr 2020 based on four databases using English and Chinese languages. We reviewed and analyzed the relevant clinic outcomes of COVID-19.Results: The COVID-19 pandemic was found to have a higher transmission rate compared to SARS and MERS and involved 4 stages of evolution. The basic reproduction number (R0) is 3.32 (95% CI:3.24-3.39), the incubation period was 5.24 days (95% CI:3.97-6.50, 5 studies) on average, and the average time for symptoms onset varied by countries. Common clinical spectrums identified included fever (38.1-39.0℃), cough and fatigue, with Acute Respiratory Distress Syndrome (ARDS) being the most common complication reported. Body temperatures above 39.0 ℃, dyspnea, and anorexia were more common symptoms in severe patients. Aged over 60 years old, having co-morbidities, and developing complications were the commonest high-risk factors associated with severe conditions. Leucopenia and lymphopenia were the most common signs of infection while liver and kidney damage were rare but may cause bad outcomes for patients. The bilateral, multifocal Ground-Glass Opacification (GGO) on peripheral, and the consolidative pulmonary opacity were the most frequent CT results and the tendency of mortality rates differed by region.Conclusions: We provided a bird’s-eye view of the COVID-19 during the current pandemic, which will help better understanding the key traits of the disease. The findings could be used for disease’s future research, control and prevention.
Molecular characterization of Plasmodium falciparum DNA-3-methyladenine glycosylase
Authors: Pinthong Nattapon , Limudomporn Paviga , Vasuvat Jitlada , Adisakwattana Poom , Rattaprasert Pongruj , Chavalitshewinkoon-Petmitr Porntip 
Published: August 2020

Abstract
Background The emergence of artemisinin-resistant malaria parasites highlights the need for novel drugs and their targets. Alkylation of purine bases can hinder DNA replication and if unresolved would eventually result in cell death. DNA-3-methyladenine glycosylase (MAG) is responsible for the repair of those alkylated bases. Plasmodium falciparum (Pf)MAG was characterized for its potential for development as an anti-malarial candidate.Methods Native PfMAG from crude extract of chloroquine- and pyrimethamine-resistant P. falciparum K1 strain was partially purified using three chromatographic procedures. From bio-informatics analysis, primers were designed for amplification, insertion into pBAD202/D-TOPO and heterologous expression in Escherichia coli of recombinant PfMAG. Functional and biochemical properties of the recombinant enzyme were characterized. Results PfMAG activity was most prominent in parasite schizont stages, with a specific activity of 147 U/mg (partially purified) protein. K1 PfMAG contained an insertion of AAT (coding for asparagine) compared to 3D7 strain and 16% similarity to the human enzyme. Recombinant PfMAG (74 kDa) was twice as large as the human enzyme, preferred double-stranded DNA substrate, and demonstrated glycosylase activity over a pH range of 4-9, optimal salt concentration of 100-200 mM NaCl but reduced activity at 250 mM NaCl, no requirement for divalent cations, which were inhibitory in a dose-dependent manner.Conclusio PfMAG activity increased with parasite development being highest in the schizont stages. K1 PfMAG contained an indel AAT (asparagine) not present in 3D7 strain and the native enzyme was twice as large as the human enzyme. Recombinant PfMAG had a wide range of optimal pH activity, and was inhibited at high (250 mM) NaCl concentration as well as by divalent cations. The properties of PfMAG provide basic data that should be of assistance in developing anti-malarials against this potential parasite target.
Lead Time Bias as a Factor in Mammography Detected Invasive Breast Cancer Survival in an Institutional Cohort
Authors: Kaplan Henry G. , Malmgren Judith , Atwood Mary K. 
Published: August 2020

Abstract
Background: Lead time, the interval between screen detection and when a disease would have become clinically evident, has been cited to explain longer survival times in mammography detected breast cancer cases (BC). Methods: An institutional retrospective cohort study of BC outcomes related to detection method (mammography (MamD) vs. patient (PtD)). Cases were first primary invasive stage I-III BC, age 40-74 years (n = 6603), 1999-2016. Survival time was divided into 1) distant disease-free interval (DDFI) and 2) distant disease-specific survival (DDSS) as two separate time interval outcomes. We measured statistical association between detection method and diagnostic, treatment and outcome variables using bivariate comparisons, Cox proportional hazards analyses and mean comparisons. Outcomes were distant recurrence (n=422), DDFI and DDSS. Results: 39% of cases were PtD (n = 2566) and 61% were MamD (n = 4037). MamD cases had a higher percentage of Stage I tumors [MamD 69% stage I vs. PtD 31%, p<.001]. Rate of distant recurrence was 11% among PtD BC cases (n=289) vs. 3% of MamD (n=133) (p<.001). Order of factor entry into the distant recurrence time interval (DDFI) model was 1) TNM stage (p<.001), 2) HR/HER2 status (p<.001), 3) histologic grade (p=.005) and 4) detection method (p<.001). Unadjusted PtD DDFI mean time was 4.34 years and MamD 5.52 years (p<.001) however when stratified by stage, the most significant factor relative to distant recurrence, there was no significant difference between PtD and MamD BC. Distant disease specific survival time did not differ by detection method.Conclusion: We observed breast cancer survival differential lead time to be a function of stage at diagnosis and tumor characteristics with marginal contribution of detection method. Patient and mammography detected breast cancer time to distant recurrence did not differ stratified by stage indicating survival difference is more likely related to early diagnosis than lead time bias. Lead time bias associated with breast cancer detection method appears to have marginal influence on survival in the current diagnostic and treatment era.
Pneumothorax in patients with idiopathic pulmonary fibrosis: A real-world experience
Authors: Yamazaki Ryo , Nishiyama Osamu , Gose Kyuya , Saeki Sho , Sano Hiroyuki , Iwanaga Takashi , Tohda Yuji 
Published: August 2020

Abstract
Background: Some patients with idiopathic pulmonary fibrosis (IPF) develop pneumothorax. However, the characteristics of pneumothorax in patients with IPF have not been elucidated. The purpose of this study was to clarify the clinical course, actual management, and treatment outcomes of pneumothorax in patients with IPF.Methods: Consecutive patients with IPF who were admitted for pneumothorax between January 2008 and December 2018 were included. The success rates of treatment for pneumothorax, hospital mortality, and recurrence rate after discharge were examined.Results: During the study period, 36 patients with IPF were admitted with pneumothorax a total of 59 times. During the first admission, 15 patients (41.7%) did not receive chest tube drainage, but 21 (58.3%) did. Of the 21 patients, 8 (38.1%) received additional therapy after chest drainage. The respective treatment success rates were 86.6% and 66.7% in patients who underwent observation only vs chest tube drainage. The respective hospital mortality rates were 13.3% and 38.0%. The total pneumothorax recurrence rate after hospital discharge was 53.8% (n = 14).Conclusions: Pneumothorax in patients with IPF was difficult to treat successfully, had a relatively poor prognosis, and showed a high recurrence rate.
Health status, lifestyle habits, and perceived social support in long-term cancer survivors: a cross-sectional study
Authors: León-Salas Beatriz , Zabaleta-del-Olmo Edurne , LLobera Joan , Bolíbar-Ribas Bonaventura , López-Jiménez Tomas , Casajuana-Closas Marc , Cantó Magdalena Esteva 
Published: August 2020

Abstract
Objective: To compare the presence of comorbidities and self-perceived health and social support between long-term cancer survivors and people without a history of cancer from a clinical trial examining the effects of a multiple risk behavior intervention.Results: Of the 4259 people studied, 190 (4.46%) were cancer survivors. They had a mean ± SD age of 62.8±7 years vs. 58.7±8 years (P<0.01) for non-cancer people and were more likely to be on long-term sick leave (11.9% vs. 3.5%, P<0.001). No differences were observed for smoking, adherence to the Mediterranean diet, physical activity, obesity, or social support. Cancer survivors were more likely to have worse self-perceived health (OR 1.82; 95% CI 1.02–2.75), more comorbidities (OR 1.68; 95% CI 1.18–2.39), COPD (OR 2.17; 95% CI 1.25–3.78), and depression (OR 1.65; 95% CI 1.06–2.57). Older age and worse self-perceived health were independent predictors of survivorship in the adjusted analysis.
MiR-4310 induced by SP1 targets PTEN to promote glioma progression
Authors: Zhiyong Wu , Jie Luo , Tengyue Huang , Renhui Yi , Shengfeng Ding , Cheng Xie , An'qi Xu , Yu Zeng , Xizhao Wang , Ye Song , Hao Long 
Published: August 2020

Abstract
Background: miRNAs have been reported to be involved in multiple biological processes of gliomas. Here, we aimed to analyze miR-4310 and its correlation genes involved in the tumor progression of human glioma.
Methods: miR-4310 expression levels were examined in glioma and non-tumor brain (NB) tissues. The molecular mechanisms of miR-4310 expression and its effects on cell proliferation, migration, and invasion were explored by 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) , Transwell chamber, Boyden chamber, and western blot analyses, as well as in vivo tumorigenesis in nude mice. The relationships among miR-4310, SP1, and phosphatase and tensin homolog (PTEN) were explored by chromatin immunoprecipitation (ChIP), agarose gel electrophoresis, electrophoresis mobility shift (EMSA), and dual luciferase reporter gene assays.
Results: miR-4310 expression was upregulated in glioma tissues compared to NB. Overexpressed miR-4310 promoted glioma cell proliferation, migration, and invasion in vitro and tumorigenesis in vivo . Inhibition of miR-4310 was sufficient to reverse these results. Mechanistic analyses revealed that miR-4310 promoted glioma progression through the PI3K/AKT pathway by targeting PTEN. Additionally, SP1 induced the expression of miR-4310 by binding to its promoter region.
Conclusion: miR-4310 promotes the progression of glioma by targeting PTEN and activating the PI3K/AKT pathway meanwhile the expression of miR-4310 is induced by SP1.
Wastewater to Ecological dyeing process and bioactive compounds resources: case study of Dittrichia graveolens hydrodistillation aqueous residue
Authors: Gharred Nawres , Baaka Noureddine , Bettache Nadir , Hamdi Assia , Dbeibia Amal , Dhaouadi Hatem , Morere Alain , Menut Chantal , Dridi-Dhaouadi Sonia 
Published: August 2020

Abstract
The release of aqueous residues generated by the extraction process of essential oils presents a real risk of environmental pollution. This work aims to reduce this risk and produce value-added materials. The aqueous residue of Dittrichia graveolens (D. graveolens) hydrodistillation has been reused in two valorization ways: 1/ in the ecological dyeing. 2/ in biological field. First, a phytochemical study of the aqueous residue was carried out by determining the ployphenols and flavonoids content and high performance liquid chromatography (HPLC) analysis. Second, the eco-dyeing process with the aqueous residue was performed on the polyamide fabric, the process was optimized by the surface response methodology using Minitab software and was evaluated by the fastness tests for the optimal conditions. Third, the aqueous residue was assessed for its biological activities. Promising coloring power and biological potential of the aqueous residue showed that this last could be an important source for developing environmentally friendly natural dyes and bioactive products.
Measurement of Postpartum Blood Loss Using a New Two - Set Liquid Collection Bag for Vaginal Delivery: A Prospective, Randomized, Case Control Study
Authors: Wang Fang , Lu Nanjia , Weng Xiaofeng , Tian Yanping , Sun Shiwen , Li Baohua 
Published: August 2020

Abstract
Background Postpartum hemorrhage (PPH) is a major obstetric complication, and the real-time measurement of blood loss is important in the management and treatment of PPH. We designed a new two-set liquid collection bag (TSLCB) for measuring postpartum blood loss in vaginal delivery. The aim of this study was to evaluate the effectiveness of the TSLCB in separating the blood from the amniotic fluid during vaginal delivery and in determining the accuracy of the measured postpartum blood loss.Methods A prospective, randomized, case control study was conducted in the Women’s Hospital, Zhejiang University School of Medicine, from March 2018 to April 2018. Sixty single pregnant women with spontaneous labor at 37–41 weeks without maternal complications were randomly divided into the experimental and control groups. The TSLCB was used to evaluate separately the amount of blood and amniotic fluid. For the control group, visual estimation and traditional plastic blood-collecting consumables were used to estimate the amount of postpartum blood loss. The measured blood loss between the two groups was compared, and the association of the measured blood loss with various clinical lab indices and vital signs was investigated. Results The TSLCB (the experimental group) improved the detection of the measured blood loss compared with visual estimation and the traditional method (the control group) (p < 0.05). In the experimental group, correlation analysis showed that the measured blood loss at delivery and within 24 h of delivery was significantly associated with the decreased hemoglobin level, red blood cell count, and hematocrit level of patients (r = -0.574, -0.455, -0.437; r = 0.-595, -0.368, -0.374; p < 0.05). In the control group, only the measured blood loss within 24 h of delivery was associated with the decreased hemoglobin level (r = -0.395, p < 0.05). No blood transfusion and plasma expanders were required in the treatment of PPH for both groups.Conclusions The TSLCB can be used to accurately measure the postpartum blood loss in vaginal delivery by medical personnel.Trial registration: This trial was registered with Chinese Clinical Trial Registry (ChiCTR): ChiCTR-IOR-17012453, 23 August 2017.
Compositional and Drug-Resistance Profiling of Pathogens in Patients with Severe Acute Pancreatitis
Authors: Fan Ning , Hu Yong , Liu Shengjie , Zhao Guang , Sun Lanju , Li Chunyan , Zhao Xin , Li Yanning , Wang Jianhua , Cui Yunfeng 
Published: August 2020

Abstract
Background: Infection is one of the important causes of death in patients with severe acute pancreatitis (SAP), but the bacterial spectrum and antibiotic resistance are constantly changing. Making good use of antibiotics and controlling multi-drug-resistant (MDR) bacterial infections are important steps in improving the cure rate of SAP.Methods: A total of 171 patients were enrolled in this study; the abdominal drainage fluid, sputum, blood, bile, deep venous catheter and urine of patients were cultured, identified and tested for resistance with a blood culture apparatus and microbiological analyzer. The associated results and hospitalization data were analyzed. Results: A total of 461 strains of pathogenic bacteria were detected, including 223 (48.4%) gram-negative bacterial strains, 190 (41.2%) gram-positive bacterial strains and 48 (10.4%) fungal strains. The detection rates of resistance in gram-negative and gram-positive bacterial strains were 48.0% (107/223) and 25.3% (48/190), respectively. There were significant differences between the MDR group and the non-MDR group for the factors of precautionary antibiotic use, kinds of antibiotics used, receipt of carbapenem, tracheal intubation, hemofiltration and number of hospitalization days in the intensive care unit. Unconditional logistic regression revealed 2 risk factors for MDR bacterial infection. Conclusions: Our results illustrate that gram-negative bacteria were the most common pathogens in SAP infection, and the proportion of gram-positive bacteria increased notably. The rate of antibiotic resistance was higher than previously reported. Unconditional logistic regression analysis showed that using more types of antibiotics and the number of hospitalization days in the ICU were the risk factors associated with MDR bacterial infection.
Effects of Resuming Training Using Yoga on Physical Functions and Fitness of Taekwondo Athletes After High-intensity Training
Authors: Liu Feng , Hou Gaixia , Liang Cheng 
Published: August 2020

Abstract
Objective: This study aimed to analyze the effects of resuming training using yoga on the physical functions and fitness of Taekwondo athletes after high-intensity training.Method: A total of 50 Taekwondo athletes from this college were randomly divided into 2 groups. 25 athletes are in the control group, and they massage their muscles after high-intensity training to help recovery. Meanwhile, 25 athletes are in observation groups, and they practice yoga after high-intensity training for resuming training. After 8 weeks, the physical functions and fitness of the two groups of athletes were compared.Result: There was no statistically significant difference between the observation group and the control group before starting high-intensity training from 1st week to 8th week (P<0.05). From the 4th week to the 8th week after high-intensity training, the observation group's heart rate, heart rate after resuming training and subjective physical sensation degree scores were lower than the control group (P<0.05). After 8 weeks of resuming training in the observation group, hemoglobin was higher than the control group, blood urea nitrogen and creatine kinase was lower than the control group (P<0.05). The observation group's flexion test results of the sitting position after 4th week and 8th week of resuming training were higher than those of the control group (P<0.05).Conclusion: The use of yoga for resuming training after high-intensity training can improve the physical function and fitness of Taekwondo athletes, improve the flexibility of athletes and promote their professional skills.
Inhibition of pyruvate dehydrogenase kinase influence microbiota and metabolomic profile in pancreatic cancer xenograft mice
Authors: Adamberg Kaarel , Vilu Raivo , Pazienza Valerio 
Published: August 2020

Abstract
Objective
Despite recent advances in treatment options, pancreatic cancer remains the most deadly major cancer. Targeting metabolism represents an emerging anti-cancer strategy.
Results
Metagenomic 16S analysis was employed to explore the effect of Dichloroacetate (DCA) on the composition of the fecal microbiota and metabolomic profile was assessed on in vivo pancreatic cancer mouse xenograft model. Pancreatic cancer xenograft mice displayed a shift of microbiota’ profile as compared to control mice without DCA treatment and a significant decrease of the purine bases inosine xanthine together with their metabolically-related compound hypoxanthine were observed in the DCA treated group as compared to the control group. Two aminoacids methionine and aspartic acid resulted decreased and increased respectively. DCA affects tumor environment and studies are needed in order to understand whether DCA supplementation could be supportive as synergistic approach to enhance the efficacy of existing cancer treatments in pancreatic cancer patients.
Evaluation of the effects of an interdisciplinary lifestyle and health program for adolescents and its benefits for maintaining health.
Authors: Schuster Anja , Schwenoha Karin , Oberkofler Edith , Lirk Gerald , Ardelt-Gattinger Elisabeth , Weghuber Daniel , Ring-Dimitriou Susanne , Freudenthaler Thomas , Oostingh Gertie J. , Bogner Barbara 
Published: August 2020

Abstract
The authors have withdrawn this preprint due to erroneous posting.
The Evaluation of Degeneration of Posterior Cruciate Ligament Using CT Hounsfield Unit in Knee Osteoarthritis
Authors: Sumida Yoshikazu , Nakasa Tomoyuki , Ishikawa Masakazu , Nakamae Atsuo , Adachi Nobuo 
Published: August 2020

Abstract
Background The degeneration of posterior cruciate ligament (PCL) is often seen in knee osteoarthritis (OA), however there is no established method for its evaluation. The purpose of this study is to investigate whether Hounsfield unit (HU) using CT (computed tomography) could be a useful scale to evaluate the degeneration of PCL in knee OA.Methods Knee OA treated with total knee arthroplasty (21 patients, 21 knees) and non-osteoarthritic knees (21 patients, 21 knees) were prospectively studied. All PCLs in the knees were analyzed by CT scan. PCL in sagittal section was divided into three regions: proximal, middle and distal portions. The HU value of PCL at each area was measured. In osteoarthritic knees, tissues from PCL were collected and histologically graded.Results The HU value in OA and non-OA was 70.7 and 86.5 HU (p < 0.05) at proximal region, 75.7 HU and 86.6 (p < 0.05) at central region and 82.3 and 88.1 HU (p > 0.05) at distal region, respectively. The degeneration of PCL was graded as follows; mild: 1 case, moderate: 3 cases and severe: 17 cases at proximal portion and mild: 16 cases, moderate: 4 cases and severe: 1 case at distal portion. The HU value was 84.5 HU for mild, 72.1 HU for moderate and 70.6 HU for severe (mild versus moderate: p < 0.05, mild versus severe: p < 0.05, moderate versus severe: p > 0.05).Conclusions In knee OA, lower HU value in PCL indicates progression of degeneration. The CT HU value could be a useful measurement to predict the grade of degeneration of PCL.