bluuebunny/crossref_metadata_2025_split huggingface dataset:
Observed Subdecadal Variations of European Summer Temperatures
Authors: Müller W.A. , Borchert L. , Ghosh R. 
Published: December 2019

AbstractWe identify subdecadal variations in European summer temperatures in coupled and uncoupled century‐long reanalyses. Spectral analyses reveal significant peaks at 5–10 years in the midtwentieth century. The subdecadal variations show substantial amplitudes of ~1–1.5 °C, associated with extremely warm summers during their positive phases. We use forced ocean model experiments and show that the European summer temperature variations are associated with the subdecadal coupled North Atlantic climate system. A positive winter NAO‐like forcing is associated with changes in the ocean circulation and mass and heat convergence occurring 1–2 years prior to European summer temperature rise. Ocean heat content and sea surface temperature increase in the subtropical North Atlantic. The atmospheric response is barotropic and induces wave activity fluxes toward the European continent, modulation of the jet positions, and blocking frequency. The atmospheric response establishes a pathway connecting the subdecadal coupled North Atlantic climate system to European summer temperature.
Solar Wind Deflection in the Foreshock: Model‐Data Comparison
Authors: Gutynska O. , Omidi N. , Sibeck D. G. , Nemecek Z. , Safrankova J. , Lynnyk A. 
Published: December 2019

AbstractWe present the results of a 2.5‐D (2‐D in space and 3‐D in currents and electromagnetic fields) electromagnetic hybrid simulation run for foreshock wave activity during an interval of a radial interplanetary magnetic field (IMF). The simulation predicts nonlinear waves and foreshock expansion in connection with the development of cavitons and the formation of the foreshock compressional boundary. Fluctuations of the ion velocity
component are associated with wave fronts corresponding to small wave normal angles and oscillate around 0 in the far upstream region. At distances closer to the bow shock, these fluctuations become asymmetric with duskward flow perturbations dominating at dusk and dawnward flow perturbations at dawn. We compare this simulation with THEMIS observations of large‐amplitude quasi‐monochromatic variations of ion velocity in the foreshock under radial IMF. A case study of simultaneous dual observations of unipolar
fluctuations is followed by a statistical study of similar solar wind deflections in the foreshock. The observations that confirm the simulation predictions indicate that, in addition to ion heating, ultralow‐frequency waves participate in the magnetosheath‐like plasma flow deflection in the foreshock during periods of small IMF cone angles.
Quasiperiodic Saturn Auroral Hiss Observed During a Cassini Proximal Orbit
Authors: Menietti J. D. , Palmaerts B. , Zahlava J. , Averkamp T. F. , Groene J. B. , Kurth W. S. 
Published: December 2019

AbstractSaturn auroral hiss is intense whistler mode emission similar in morphology to terrestrial auroral hiss, and is observed at high latitude very often in quasiperiodic episodes with a period of approximately 1 hr. Bader et al. (2019) report auroral pulsations that may be due to duskside magnetodisk reconnection. The source of the 1‐hr period is not definitively known but has been purported to be due to second harmonic Alfven waves standing along near planet magnetic field lines (Yates et al., 2016). Observations of auroral hiss at high latitude along Cassini proximal orbits are often excellent, and we have focused on an event for which we have concurrent ultraviolet auroral images as well as electron flux data. A series of repeating auroral hiss episodes is observed to initiate near the magnetic field line that traverses a Saturn kilometric radiation source region in each hemisphere, with periodic episodes of hiss recurring at higher L‐shells. Magnetic field lines centered on individual hiss episodes have auroral footprints that lie near and within a region of intense auroral ultraviolet emissions. These observations have a parallel in terrestrial return current electron beam‐generated auroral hiss seen near magnetic field lines supporting auroral kilometric radiation source regions. Recent findings link periodic plasma injections with Saturn reconnection sites observed preferentially on the duskside. These injections may spawn Saturn kilometric radiation source regions and periodic auroral hiss emission in nearby return current regions.
Equatorial Magnetosonic Waves: Do Nonlinear Interactions Play a Role in Their Evolution?
Authors: Walker S. N. , Balikhin M. A. , Yearby K. H. , Aryan H. 
Published: December 2019

AbstractThe occurrence of nonlinear interactions between discrete wave frequencies has been shown to play a significant role in the propagation and evolution of some plasma wave modes. In this paper we take advantage of closely spaced Cluster measurements to investigate the possibility nonlinear interactions occurring between the discrete emissions that are observed in a region of Equatorial Magnetosonic Wave generation. Based on transfer function analysis, it is shown that the role of nonlinear interactions plays a negligible role in the wave evolution as the emissions propagate from one satellite to the other. A bicoherence analysis of the individual signals also fails to find the existence of nonlinear interactions in the evolution of equatorial magnetosonic waves.
Magnesium and Lithium Isotopic Evidence for a Remnant Oceanic Slab Beneath Central Tibet
Authors: Tian Heng‐Ci , Teng Fang‐Zhen , Hou Zeng‐Qian , Tian Shi‐Hong , Yang Wei , Chen Xin‐Yang , Song Yu‐Cai 
Published: December 2019

AbstractThe voluminous Eocene postcollisional potassium‐rich magmatism in central Tibet is crucial in understanding the deep geodynamic process and related uplift history of the Tibetan Plateau. Here, we present an integrated study of whole‐rock Sr‐Nd‐Mg‐Li isotopes and zircon U‐Pb ages on two suites of lavas from Duocaima and Nariniya in the northern Qiangtang block. The Duocaima high‐K calc‐alkaline trachyandesites were formed at ~35 Ma and are enriched in light rare earth elements and large ion lithophile elements. They have slightly lower δ26Mg (−0.41‰ to −0.33‰) and δ7Li (+1.0‰ to +2.6‰) relative to the mantle. Together with their high 87Sr/86Sr(i) (0.7066–0.7067) and low ɛNd(t) (−1.96 to −1.61) values, they most likely originated from the lithospheric mantle previously metasomatized by melts from subducted carbonate‐bearing sediments. Model calculation suggests that ~7–11% sediments were added into the mantle via melting. By contrast, the contemporaneous Nariniya adakitic trachytes have higher δ26Mg (−0.13‰ to −0.02‰) and δ7Li (+3.3‰ to +5.4‰) values, as well as higher Pb/Ce and Ba/La ratios, which most likely resulted from the thickened lower crust that was metasomatized by fluids released from downgoing Songpan‐Ganzi oceanic slab. Our combined isotopic study suggests the existence of a remnant oceanic slab beneath central Tibetan Plateau during the late Eocene and implies that the initial uplift of central Tibet was before ~38 Ma.
Enhancement of Atmospheric Stability by Anomalous Elevated Aerosols During Winter in China
Authors: Lu Xin , Mao Feiyue , Pan Zengxin , Gong Wei , Zhu Yannian , Yang Jie 
Published: December 2019

AbstractChanges in anthropogenic aerosols with a low level of scientific cognition affect the atmospheric environment and radiative balance of the Earth. We determine anomalous elevated aerosol layers (EALs) and their source and effects on regional atmospheric stability in China by using 2007–2016 data from multiple satellite observations and models. Anomalous EALs occur during winter over Eastern China, and they are mainly distributed above 2 km and can even spread up to 4–5 km despite the disadvantageous atmospheric convection conditions during winter. Anthropogenic pollutants that mainly originate from local‐range transport with enhanced atmospheric updraft play a dominant role (about 60%) in this situation. Furthermore, anomalous EALs result in considerable warming in the upper atmosphere (+0.32 K/day) and cooling on the surface (−0.04 K/day) over Eastern China. They decrease surface incident radiation by an average of 3.4 W/m2. The opposite heating rate change in the upper atmosphere and surface caused by anomalous EALs consequently increases the low atmospheric stability by an average of 0.39 K, and this increment weakens the dissipation of atmospheric dispersion. Related studies have indicated that increased atmospheric stability can severely exacerbate atmospheric pollution near the surface.
Laboratory and Numerical Investigation of Saltwater Intrusion Processes in a Circular Island Aquifer
Authors: Memari Sama S. , Bedekar Vivek S. , Clement T. Prabhakar 
Published: December 2019

AbstractA novel radial flow experimental system to study saltwater intrusion processes in an island aquifer is presented. The study investigated steady state and transient scenarios involving advancing and receding saltwater wedges in a circular island. The experimental results were simulated using the density‐coupled version of the MODFLOW‐USG code. The experimental data along with the model simulation results are employed to develop a new radial benchmark problem for testing density‐coupled models used for simulating saltwater intrusion processes. The experimental data for transient changes in toe position and freshwater storage level indicated an asymmetric pattern where the intrusion time scale is greater than the recession time scale. Numerical experiments were completed to further investigate this asymmetric effect and to intercompare the associated transient transport processes in circular and linear strip islands. We also analyzed the sensitivity of island geometry in controlling the freshwater storage levels under different recharge conditions. Modeling results show that for similar‐sized systems, circular islands are more efficient in storing freshwater than linear strip islands.
Characterization and printability of Sodium alginate -Gelatin hydrogel for bioprinting NSCLC co-culture
Authors: Mondal Arindam , Gebeyehu Aragaw , Miranda Mariza , Bahadur Divya , Patel Nilkumar , Ramakrishnan Subhramanian , Rishi Arun K. , Singh Mandip 
Published: December 2019

Abstract3D bioprinting improves orientation of in vitro tumor models by offering layer by layer positioning of cancer cells and cancer associated fibroblasts (CAFs) which can replicate tumor microenvironment. Aim of this study was to develop a sodium alginate -gelatin (SA-GL) hydrogel by optimizing rheological parameters to print non-small cell lung cancer (NSCLC) patient derived xenograft (PDX) cells and lung CAFs co-cultures. SA-GL hydrogels were prepared, and rheological properties were evaluated. Both the cells were mixed with the hydrogel and printed using INKREDIBLE bioprinter. Hydrogels prepared with 3.25% and 3.5% (w/v) SA and 4% (w/v) GL showed higher printability and cell viability. A significant decline in viscosity with shear rate was observed in these hydrogels suggesting the shear thinning property of hydrogels. Spheroid size distribution after 15 days was in the diameter range of 50–1100 µm. Up-regulation of vimentin, α-SMA and loss of E-cadherin in co-culture spheroids confirmed cellular crosstalk. This study demonstrates that rheological optimization of SA-GL hydrogel enhances printability and viability of NSCLC PDX and CAF co-culture which allows 3D co-culture spheroid formation within the printed scaffold. Therefore, this model can be used for studying high throughput drug screening and other pre-clinical applications.
Quantitative MRI Biomarkers of Stereotactic Radiotherapy Outcome in Brain Metastasis
Authors: Karami Elham , Soliman Hany , Ruschin Mark , Sahgal Arjun , Myrehaug Sten , Tseng Chia-Lin , Czarnota Gregory J. , Jabehdar-Maralani Pejman , Chugh Brige , Lau Angus , Stanisz Greg J. , Sadeghi-Naini Ali 
Published: December 2019

AbstractAbout 20–40% of cancer patients develop brain metastases, causing significant morbidity and mortality. Stereotactic radiation treatment is an established option that delivers high dose radiation to the target while sparing the surrounding normal tissue. However, up to 20% of metastatic brain tumours progress despite stereotactic treatment, and it can take months before it is evident on follow-up imaging. An early predictor of radiation therapy outcome in terms of tumour local failure (LF) is crucial, and can facilitate treatment adjustments or allow for early salvage treatment. In this study, an MR-based radiomics framework was proposed to derive and investigate quantitative MRI (qMRI) biomarkers for the outcome of LF in brain metastasis patients treated with hypo-fractionated stereotactic radiation therapy (SRT). The qMRI biomarkers were constructed through a multi-step feature extraction/reduction/selection framework using the conventional MR imaging data acquired from 100 patients (133 lesions), and were applied in conjunction with machine learning techniques for outcome prediction and risk assessment. The results indicated that the majority of the features in the optimal qMRI biomarkers characterize the heterogeneity in the surrounding regions of tumour including edema and tumour/lesion margins. The optimal qMRI biomarker consisted of five features that predict the outcome of LF with an area under the curve (AUC) of 0.79, and a cross-validated sensitivity and specificity of 81% and 79%, respectively. The Kaplan-Meier analyses showed a statistically significant difference in local control (p-value < 0.0001) and overall survival (p = 0.01). Findings from this study are a step towards using qMRI for early prediction of local failure in brain metastasis patients treated with SRT. This may facilitate early adjustments in treatment, such as surgical resection or salvage radiation, that can potentially improve treatment outcomes. Investigations on larger cohorts of patients are, however, required for further validation of the technique.
Experimental arthritis and Porphyromonas gingivalis administration synergistically decrease bone regeneration in femoral cortical defects
Authors: Okumura Go , Kondo Naoki , Sato Keisuke , Yamazaki Kazuhisa , Ohshima Hayato , Kawashima Hiroyuki , Ogose Akira , Endo Naoto 
Published: December 2019

AbstractPorphyromonas gingivalis infection can lead to periodontitis and dysbiosis, which are known risk factors for rheumatoid arthritis (RA). We investigated whether P. gingivalis administration affected bone regeneration in mice with or without arthritis. We administered P. gingivalis to male DBA/1 J mice that were or were not sensitised to type II collagen-induced arthritis (CIA). All mice underwent drilling of bilateral femurs. We histologically evaluated new bone regeneration (bone volume of the defect [BVd]/tissue volume of the defect [TVd]) using micro-computed tomography (micro-CT), osteoclast number/bone area, and active osteoblast surface/bone surface (Ob.S/BS). We measured serum cytokine levels and bone mineral density of the proximal tibia using micro-CT. CIA resulted in significantly reduced bone regeneration (BVd/TVd) at all time-points, whereas P. gingivalis administration showed similar effects at 2 weeks postoperatively. CIA resulted in higher osteoclast number/bone area and lower Ob.S/BS at 2 and 3 weeks postoperatively, respectively. However, P. gingivalis administration resulted in lower Ob.S/BS only at 2 weeks postoperatively. During later-stage bone regeneration, CIA and P. gingivalis administration synergistically decreased BVd/TVd, increased serum tumour necrosis factor-α, and resulted in the lowest bone mineral density. Therefore, RA and dysbiosis could be risk factors for prolonged fracture healing.
A CRISPR-Cas9 System for Genome Editing of Fusarium proliferatum
Authors: Ferrara Massimo , Haidukowski Miriam , Logrieco Antonio F. , Leslie John F. , Mulè Giuseppina 
Published: December 2019

AbstractFusarium proliferatumcauses diverse diseases of many economically important plants. The fungus produces several mycotoxins of which the fumonisins are the most toxic. Currently, deletion of key genes for mycotoxin biosynthesis is a laborious and time-consuming procedure. We developed a novel CRISPR/Cas9-based genome-editing tool for the direct delivery of preassembled Cas9 ribonucleoproteins into protoplasts ofF. proliferatum. Our CRISPR–Cas9 system couples a site-specific double-strand DNA break mediated by two Cas9 ribonucleoproteins with microhomology recombination requiring only 50-bp regions flanking the target gene. This system reduces the risk of off-target mutations and minimizes the risk of altering any gene adjacent to the target region. We used this tool to delete a polyketide synthase gene (FUM1) required for fumonisin biosynthesis. The mutants generated are no longer able to produce fumonisins, confirming the key role ofFUM1in fumonisin biosynthesis. Our CRISPR-Cas9 system is an important new tool for genetic studies ofFusarium.
Dissecting multi drug resistance in head and neck cancer cells using multicellular tumor spheroids
Authors: Azharuddin Mohammad , Roberg Karin , Dhara Ashis Kumar , Jain Mayur Vilas , Darcy Padraig , Hinkula Jorma , Slater Nigel K. H. , Patra Hirak K. 
Published: December 2019

AbstractOne of the hallmarks of cancers is their ability to develop resistance against therapeutic agents. Therefore, developing effective in vitro strategies to identify drug resistance remains of paramount importance for successful treatment. One of the ways cancer cells achieve drug resistance is through the expression of efflux pumps that actively pump drugs out of the cells. To date, several studies have investigated the potential of using 3-dimensional (3D) multicellular tumor spheroids (MCSs) to assess drug resistance; however, a unified system that uses MCSs to differentiate between multi drug resistance (MDR) and non-MDR cells does not yet exist. In the present report we describe MCSs obtained from post-diagnosed, pre-treated patient-derived (PTPD) cell lines from head and neck squamous cancer cells (HNSCC) that often develop resistance to therapy. We employed an integrated approach combining response to clinical drugs and screening cytotoxicity, monitoring real-time drug uptake, and assessing transporter activity using flow cytometry in the presence and absence of their respective specific inhibitors. The report shows a comparative response to MDR, drug efflux capability and reactive oxygen species (ROS) activity to assess the resistance profile of PTPD MCSs and two-dimensional (2D) monolayer cultures of the same set of cell lines. We show that MCSs provide a robust and reliable in vitro model to evaluate clinical relevance. Our proposed strategy can also be clinically applicable for profiling drug resistance in cancers with unknown resistance profiles, which consequently can indicate benefit from downstream therapy.
A natural polymorphism in Zika virus NS2A protein responsible of virulence in mice
Authors: Ávila-Pérez Gines , Nogales Aitor , Park Jun-Gyu , Márquez-Jurado Silvia , Iborra Francisco J. , Almazan Fernando , Martínez-Sobrido Luis 
Published: December 2019

AbstractZika virus (ZIKV) infection is currently one of the major concerns in human public health due to its association with neurological disorders. Intensive effort has been implemented for the treatment of ZIKV, however there are not currently approved vaccines or antivirals available to combat ZIKV infection. In this sense, the identification of virulence factors associated with changes in ZIKV virulence could help to develop safe and effective countermeasures to treat ZIKV or to prevent future outbreaks. Here, we have compared the virulence of two related ZIKV strains from the recent outbreak in Brazil (2015), Rio Grande do Norte Natal (RGN) and Paraiba. In spite of both viruses being identified in the same period of time and region, significant differences in virulence and replication were observed using a validated mouse model of ZIKV infection. While ZIKV-RGN has a 50% mouse lethal dose (MLD50) of ~105 focus forming units (FFUs), ZIKV-Paraiba infection resulted in 100% of lethality with less than 10 FFUs. Combining deep-sequencing analysis and our previously described infectious ZIKV-RGN cDNA clone, we identified a natural polymorphism in the non-structural protein 2 A (NS2A) that increase the virulence of ZIKV. Moreover, results demonstrate that the single amino acid alanine to valine substitution at position 117 (A117V) in the NS2A was sufficient to convert the attenuated rZIKV-RGN in a virulent Paraiba-like virus (MLD50 < 10 FFU). The mechanism of action was also evaluated and data indicate that substitution A117V in ZIKV NS2A protein reduces host innate immune responses and viral-induced apoptosis in vitro. Therefore, amino acid substitution A117V in ZIKV NS2A could be used as a genetic risk-assessment marker for future ZIKV outbreaks.
Prognostic factors and outcome of adult allogeneic hematopoietic stem cell transplantation patients admitted to intensive care unit during transplant hospitalization
Authors: Michel Christian S. , Teschner Daniel , Schmidtmann Irene , Theobald Matthias , Hauptrock Beate , Wagner-Drouet Eva M. , Radsak Markus P. 
Published: December 2019

AbstractPatients undergoing allogeneic hematopoietic stem cell transplantation have a high morbidity and mortality, especially after admission to intensive care unit (ICU) during peri-transplant period. The objective of this study was to identify new clinical and biological parameters and validate prognostic scores associated with ICU, short-and long-term survival. Significant differences between ICU survivors and ICU non-survivors for the clinical parameters invasive mechanical ventilation, urine output, heart rate, mean arterial pressure, and amount of vasopressors have been measured. Among prognostic scores (SOFA, SAPSII, PICAT, APACHE II, APACHE IV) assessing severity of disease and predicting outcome of critically ill patients on ICU, the APACHE II score has shown most significant difference (p = 0.002) and the highest discriminative power (area under the ROC curve (AUC) 0.74). An elevated level of lactate at day of admission was associated with poor survival on ICU and the most significant independent parameter (p < 0.001). In our cohort kidney damage with low urine output has a highly relevant impact on ICU, short- and long-term overall survival. The APACHE II score was superior predicting ICU mortality compared to all other tested prognostic scores for patients on ICU during peri-transplant period.
Differentiating external zeitgeber impact on peripheral circadian clock resetting
Authors: Heyde Isabel , Oster Henrik 
Published: December 2019

AbstractCircadian clocks regulate physiological functions, including energy metabolism, along the 24-hour day cycle. The mammalian clock system is organized in a hierarchical manner with a coordinating pacemaker residing in the hypothalamic suprachiasmatic nucleus (SCN). The SCN clock is reset primarily by the external light-dark cycle while other zeitgebers such as the timing of food intake are potent synchronizers of many peripheral tissue clocks. Under conflicting zeitgeber conditions, e.g. during shift work, phase synchrony across the clock network is disrupted promoting the development of metabolic disorders. We established a zeitgeber desynchrony (ZD) paradigm to quantify the differential contributions of the two main zeitgebers, light and food, to the resetting of specific tissue clocks and the effect on metabolic homeostasis in mice. Under 28-hour light-dark and 24-hour feeding-fasting conditions SCN and peripheral clock, as well as activity and hormonal rhythms showed specific periodicities aligning in-between those of the two zeitgebers. During ZD, metabolic homeostasis was cyclic with mice gaining weight under synchronous and losing weight under conflicting zeitgeber conditions. In summary, our study establishes an experimental paradigm to compare zeitgeber input in vivo and study the physiological consequences of chronodisruption.