bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transgelin interacts with PARP1 and affects Rho signaling pathway in human colon cancer cells
Authors: Lew Zhen-xian , Zhou Hui-min , Fang Yuan-yuan , Ye Zhen , Zhong Wa , Yang Xin-yi , Yu Zhong , Chen Dan-yu , Luo Si-min , Chen Li-fei , Lin Ying 
Published: March 2020

Abstract
Background: Transgelin, an actin-binding protein, is associated with the cytoskeleton remodeling. Our previous studies found that transgelin was up-regulated in node-positive colorectal cancer versus in node-negative disease. Over-expression of TAGLN affected the expression of 256 downstream transcripts and increased the metastatic potential of colon cancer cells in vitro and in vivo. This study aims to explore the mechanisms that transgelin participates in the metastasis of colon cancer cells. Methods: Immunofluorescence and immunoblotting analysis were used to determine the cellular localization of the endogenous and exogenous transgelin in colon cancer cells. Co-immunoprecipitation and subsequent high performance liquid chromatography/tandem mass spectrometry were performed to identify the proteins potentially interacting with transgelin. Bioinformatics methods were used to analyze the 256 downstream transcripts regulated by transgelin to discriminate the specific key genes and signaling pathways. By analyzing the promoter region of these key genes, GCBI tools were used to predict the potential transcription factor(s) for these genes. The predicted transcription factors were matching to the proteins that have been identified to potentially interact with transgelin. The interaction between transgelin and these transcription factors was verified by co-immunoprecipitation and immunoblotting. Results: Transgelin was found to localize both in the cytoplasm and the nucleus of colon cancer cells. 297 proteins have been identified to interact with transgelin by co-immunoprecipitation and subsequent high performance liquid chromatography/mass spectrometry. Over-expression of TAGLN could lead to differential expression of 184 downstream genes. By constructing the network of gene-encoded proteins, 7 genes ( CALM1, MYO1F, NCKIPSD, PLK4, RAC1, WAS and WIPF1 ) have been discriminated as key genes using network topology analysis. They are mostly involved in the Rho signaling pathway. Poly ADP-ribose polymerase-1 (PARP1) was predicted as the unique transcription factor for the key genes and concurrently matching to the DNA-binding proteins potentially interacting with transgelin. Immunoprecipitation validated that PARP1 interacted with transgelin in human RKO colon cancer cells. Conclusions: The results of this study suggest that transgelin binds to PARP1 and regulates the expression of the downstream key genes mainly involving Rho signaling pathway, thus participates in the metastasis of colon cancer.
WITHDRAWN: Expression of somatostatin receptors subtypes (1-5) mRNA in MCF7 and MDA-MB231 breast cancer human cell lines
Authors: MAHMOUD ALKHANSA , Mancuso Maria Teresa , Tanno Barbara , Bakar Zuki Abu , Hamzah Hazilawati , Hezmee Mohd 
Published: March 2020

Abstract
Background: breast cancer is one of most common types of cancer and heterogeneous disease. Somatostatain receptors (SSTR1-5) are expressed in breast cancer cells. MCF7 and MDA-MB231 cell lines used to evaluate the expression of somatostatin receptors (SSTRs 1 - 5). Methods: The detection of mRNA expression levels of SSTRs in MCF7 and MDA-MB231 was performed using quantitative- polymerase chain reaction (qPCR). Results: All SSTRs 1 - 5 were expressed in both cells lines. The SSTR 1, 2, 3 and 4 mRNA levels were significantly higher in MDA-MB-231 in relation to MCF-7. The expression of SSTR 4 and 5 mRNA was highest in MDA-MB231 and MCF-7 cell lines respectively, however, SSTR3 mRNA was least expressed in both cell lines. Conclusion: All SSTRs were expressed in both MDA-MB231 and MCF7 cells. However, the levels of expression differ between both cell lines. Keywords: Breast cancer. Cell lines .Somatostatin receptors. mRNA expression
Association between serum progesterone levels on the day of frozen-thawed embryo transfer and pregnancy outcomes after artificial endometrial preparation
Authors: Zhu Jing , Zhu Qianqian , Huang Jialyu , Qiu Meiting , Zhu Yanwen , Cai Renfei , Kuang Yanping 
Published: March 2020

Abstract
Background Previous studies have examined that a range of optimal serum P level during the implantation period was associated with optimal live birth rates. However, those results obtained with vaginal or intramuscular route of progesterone administration for LPS alone. Is there a relationship between the serum progesterone (P) on the day of frozen-thawed embryo transfer (FET) with the likelihood of a live birth(LB) in artiﬁcial cycles(AC) when using a combination of oral dydrogesterone and vaginal progesterone for luteal phase support (LPS)? Methods This was a retrospective study of 3659 FET cycles with artiﬁcial endometrial preparation in a Chinese tertiary-care academic medical centre from January 2015 to February 2017. Endometrial preparation was performed using estradiol (E 2 ) valerate (Fematon-red tablets) 8 mg/d beginning on day 3 of the cycle, followed by administration of P both orally (40 mg dydrogesterone and 8 mg E 2 twice per day, Fematon-yellow tablets) and vaginally (400 mg/d; Utrogestan). The primary endpoint was LBR. The association between the serum P level on the embryo transfer day and pregnancy outcomes was evaluated by univariable and multivariable logistic regression analysis. Results Mean serum P on the day of embryo transfer was 10.30 ± 3.88 ng/ml (percentiles: 25, 7.9; 50, 9.7; 75, 12.1). The LBRs according to the serum P quartiles were as follows: Q1, 35.7%; Q2, 37.4%; Q3, 39.1%; and Q4: 38.9%. Logistic regression analysis showed that the odds of a LB were not significantly different between the low (P <7.9 ng/mL) and high (P ≥7.9 ng/mL) progesterone groups before or after adjustment (crude odds ratio [OR] = 0.89, 95% confidence interval [CI]: 0.76-1.04; adjusted OR = 0.89, 95% CI: 0.75-1.04). Conclusions The present study suggests that the serum P levels on the day of ET do not correlate with the likelihood of a LB in artiﬁcial cycles when using a combination of oral dydrogesterone and vaginal progesterone for luteal phase support. In addition, prospective, randomized, controlled, blinded trials are merited to determine the optimal dosing regimen for oral dydrogesterone in in AC-FET for LPS.
Date Palm Pollen versus Pentoxifylline on Improvement of Sperm Parameters in Idiopathic Male Infertility
Authors: Mirzaei Mohammadreza , Asbagh Firoozeh Akbari , Safavi Majid , Yekaninejad Mir Saeed , Rahimi Roja , Pourmand Gholamreza , Karimi Majid 
Published: March 2020

Abstract
Background: Idiopathic male infertility (IMI) affects nearly 30 to 45% of men in their optimal reproductive age. In this regard, there are no evidence-based medications prescribed empirically to treat IMI. However, applying traditional medicine for the treatment of male infertility has attracted more attention in recent years. In the current study, the effects of date palm pollen (DPP) on sperm parameters in patients with idiopathic infertility were evaluated and compared to pentoxifylline (PTX). Materials and methods: This study was performed on 80 adult male individuals (20-35 years old) who had oligozoospermia and/or asthenozoospermia and/or teratozoospermia. Patients received 6g DPP powder or 400 mg PTX tablets daily for three months in two separated groups (each in 40). Various sperm parameters and levels of sex hormones were measured. Results: Compared to the PTX group, results revealed a significant improvement in sperm counts (p=0.016), morphology (p=0.029), total motility (p=0.018), progressive motility (p=0.016) and reduction of immotile sperms (p=0.014) in patients who received DPP treatment. Conclusion Iranian Traditional Medicine (ITM) strongly recommended DPP as a therapeutic agent to cure IMI through improvement in the functional competence of sperm and semen parameters.
Neoadjuvant Laparoscopic HIPEC combined with Neoadjuvant Chemotherapy followed by Surgery plus intraoperative HIPEC for Advanced Gastric Cancer at risk of Peritoneal Carcinomatosis: feasibility and safety assessment from retrospective case control study
Authors: Beeharry Maneesh Kumarsing , Ni Zhen Tian , Liu Wen Tao , Yao Xue Xin , Yan Min , Zhu Zheng Gang 
Published: March 2020

Abstract
Objective: In this study we have retrospectively evaluated the feasibility and safety of a novel therapeutic regimen for the management of Advanced Gastric Cancer (AGC) at risk for peritoneal carcinomatosis (PC) involving neoadjuvant laparoscopic hyperthermic intraperitoneal chemotherapy (NLHIPEC) and neoadjuvant chemotherapy (NAC).Methods: From December 2017 to June 2018, 7 AGC patients undergoing NLHIPEC + NAC + Surgery+ Intraoperative HIPEC + adjuvant chemotherapy (AC) were included in the study and observed for associated complication and morbidity. AGC patients undergoing other neoadjuvant and prophylactic treatment approaches were retrospectively analyzed and case-matched. Intraoperative and post-operative events, clinical recovery and morbidity were closely monitored till 1 month after the last cycle of chemotherapy.Results: Of a total of 328 AGC patients undergoing curative surgery, 7 other patients undergoing NAC + HIPEC, 7 patients undergoing surgery + HIPEC and 17 patients undergoing NAC were retrospectively included. There was no 30-day post-operative mortality recorded in the 4 groups. In total, 14 events were recorded and 5/14 of the complications were rated as Clavien I; 8/14 patients were classified as Clavien II and 1/14 patient was recorded as Clavien grade IIIa. There was no grade IIIb-V event. The results suggest that the proposed treatment regimen is safe and feasible.Conclusions: This combination of NLHIPEC and NAC in the management of AGC at high risk for peritoneal involvement is feasible, safe, well-tolerated and worth exploring.
Expression of microRNA-26b-5p is related to the changes of random blood glucose after primary percutaneous coronary interventions
Authors: Zhang Jianwie , He Lingjie 
Published: March 2020

Abstract
Background: Stress hyperglycemia （SH） is prevalent in non-diabetic patients with ST-segment elevation myocardial infarction (STEMI), and it can significantly increase the risk of adverse cardiovascular events. The study aims to determine the changes of and correlation between circulating microRNA-26b-5p levels and random blood glucose levels after primary percutaneous coronary interventions (p-PCI).Methods: INS-1 cells were used to establish model of insulin regulation. Overexpression of miR-26b was performed with lentiviral transfection, while downregulation of miR-26b was accomplished by using shRNA sequences. Insulin was quantitatively detected by an enzyme-linked immunosorbent assay kit (ELISA).we enrolled 48 patients with acute anterior wall myocardial infarction who successfully underwent p-PCI from June 2017 to November 2017 in beijing anzhen hospital and beijing friendship hospital, capital medical university. Plasma samples were collected before p-PCI, 24h and 72h after operation. The level of circulating microRNA-26b-5p were measured by Real Time PCR. The association between circulating microRNA-26b-5p and random blood glucose was calculated by Spearman correlation coefficient.Results: The results of in vitro test showed i nhibition of miR-26b leads to a decrease in insulin secretion while overexpression of miR-26b increases insulin secretion in INS-1 cells. 34 male patients accounting for 71% were included in this study. The circulating microRNA-26b-5p levels were 14.41±8.52 and 19.63±9.42 at 24h and 72h after operation respectively, as compared with 10.32±6.62 before p-PCI ( P <0.001). Significantly decreased random blood glucose level were found at 24h and 72h after operation compared with that before p-PCI（7.35±1.17 VS. 7.12±1.17 VS. 8.28±1.35, P< 0.001. Spearman correlation coefficient revealed that there was an inverse correlation between circulating microRNA-26b-5p levels and random blood glucose levels（ r =-0.425 ， P <0.001).Conclusion: MicroRNA-26b-5p may be involved in the regulation of blood glucose in non-diabetic patients with STEMI after p-PCI.
High yield production of L-serine through a novel identified exporter combined with synthetic pathway in Corynebacterium glutamicum
Authors: Zhang Xiaomei , Gao Yujie , Chen Ziwei , Xu Guoqiang , Zhang Xiaojuan , Li Hui , Shi Jinsong , Koffas Mattheos , Xu Zhenghong 
Published: March 2020

Abstract
Background L-Serine has wide and expanding applications in industry with a fast-growing market demand. Strategies for achieving and improving L-serine production in C. glutamicum have focused on inhibiting its degradation and enhancing its biosynthetic pathway, however, L-serine yield remains relatively low. Exporters play an essential role in the fermentative production of amino acids. Improvements to L-serine yield should consider the improvement of L-serine export from the cell. In C. glutamicum , ThrE, which can export L-threonine and L-serine, is the only identified L-serine exporter so far.Results In this study, a novel L-serine exporter NCgl0580 was identified and characterized in C. glutamicum ΔSSAAI (SSAAI), and named as SerE (encoded by serE ). Deletion of serE in SSAAI led to a 56.5% decrease in L-serine titer, whereas overexpression of serE compensated for the lack of serE with respect to L-serine titer. A fusion protein with SerE and enhanced green fluorescent protein (EGFP) was constructed to confirm that SerE localized at the plasma membrane. The function of SerE was studied by peptide feeding approaches, and the results showed that SerE is a novel exporter for L-serine and L-threonine in C. glutamicum. Subsequently, the interaction of a known L-serine exporter ThrE and SerE was studied, and the results suggested that SerE is more important than ThrE in L-serine export in SSAAI. In addition, probe plasmid and electrophoretic mobility shift assays (EMSA) revealed NCgl0581 as the transcriptional regulator of SerE. Comparative transcriptomics between SSAAI and the NCgl0581 deletion strain showed that NCgl0581 is a positive regulator of NCgl0580. Finally, by overexpressing the novel exporter SerE combined with L-serine synthetic pathway key enzyme serA Δ197, serC and serB , the resulting strain lead to the L-serine titer of 43.9 g/L with yield of 0.44 g/g sucrose, which was the highest yield reported so far for any organism.Conclusions This study provides a novel target for L-serine and L-threonine export engineering as well as a novel global transcriptional regulator NCgl0581 in C. glutamicum .
Anomaly Detection from Medical Signals and Images Using Advanced Convolutional Neural Network
Authors: Abbass Mohammed , Kwon Ki-Chul , Kim Nam , Abdelwahab Safey A. , Haggag Nehad , Ibrahim Fatma , Mahrous Yasser , Seddik Ahmad , Khalil Ali , Elsherbeeny Zeinab , El-Shafai Walid , Rihan Mohamad , El-Banby Ghada , Soltan Eman , Soliman Naglaa , Algarni Abeer , Alhanafy Waleed , El-Fishawy Adel , El-Rabaie El-Sayed M. , Al-Nuaimy Waleed , Dessouky Moawad I. , Saleeb Adel A. , Messiha Nagy W. , El-Dokany Ibrahim M. , El-Samie Fathi E. Abd , Khalaf Ashraf A.M. 
Published: March 2020

Abstract
In the field of Artificial Intelligence (AI), deep learning is a method falls in the wider family of machine learning algorithms that works on the principle of learning. Convolutional Neural Networks (CNNs) can be used for pattern recognition from different images based on deep learning. Anomaly detection is a very vital area in medical signal and image processing due to its importance in automatic diagnosis. Anomaly detection from medical EEG signals based on spectrogram and medical corneal images are tested and evaluated in this paper. Technically, deep learning CNN models are used in the train and test processes, each input image will pass through a series of convolution layers with filters (Kernels), pooling, and fully connected layers (FC) for the classification purposes. The presented simulation results reveal the success of the proposed techniques towards automated medical diagnosis.
Genetic dissection of an allotetraploid interspecific CSSLs guides interspecific genetics and breeding in cotton
Authors: Zhu De , Li Ximei , Wang Zhiwei , You Chunyuan , Nie Xinhui , Sun Jie , Zhang Xianlong , Zhang Dawei , Lin Zhongxu 
Published: March 2020

Abstract
Background: The low genetic diversity of Upland cotton limits the potential for genetic improvement. Making full use of the genetic resources of Sea-island cotton will facilitate genetic improvement of widely cultivated Upland cotton varieties. The chromosome segments substitution lines (CSSLs) provide an ideal strategy for mapping quantitative trait loci (QTLs) in interspecific hybridization. Results: In this study, a CSSL population was developed by PCR-based markers assisted selection (MAS), derived from the crossing and backcrossing of Gossypium hirsutum (Gh) and G. barbadense (Gb), firstly. Then, by whole genome re-sequencing, 11,653,661 high-quality single nucleotide polymorphisms (SNPs) were identified which ultimately constructed 1,211 recombination chromosome introgression segments from Gb. The sequencing-based physical map provided more accurate introgressions than the PCR-based markers. By exploiting CSSLs with mutant morphological traits, the genes responding for leaf shape and fuzz-less mutation in the Gb were identified. Based on a high-resolution recombination bin map to uncover genetic loci determining the phenotypic variance between Gh and Gb, 64 QTLs were identified for 14 agronomic traits with an interval length of 158 kb to 27 Mb. Surprisingly, multiple alleles of Gb showed extremely high value in enhancing cottonseed oil content (SOC). Surprisingly, multiple alleles of Gb showed extremely high value in enhancing cottonseed oil content (SOC). Conclusions: This study provides guidance for studying interspecific inheritance, especially breeding researchers, for future studies using the traditional PCR-based molecular markers and high-throughput re-sequencing technology in the study of CSSLs. Available resources include candidate position for controlling cotton quality and quantitative traits, and excellent breeding materials. Collectively, our results provide insights into the genetic effects of Gb alleles on the Gh, and provide guidance for the utilization of Gb alleles in interspecific breeding.
Transcriptome analysis of in vitro fertilization and parthenogenesis activation during early embryonic development in pigs
Authors: Li Xin , Huang Ziying , Zou Cheng , Li Mengxun , Liu Xiangdong , Zhao Shuhong , Li Changchun 
Published: March 2020

Abstract
BackgroundParthenogenesis-activated oocytes exhibit inferior embryonic development and lower total cell count per blastocyst than fertilized embryos, thus often leading to pre-implantation failure in porcine. The mechanisms underlying the deficiencies of embryos generated from parthenogenesis activation have not been completely understood. Alternative splicing events (AS) and long intergenic non-coding RNAs (lincRNAs) have emerged as key regulators in various biological processes. However, their regulatory mechanisms for parthenogenesis have not been fully elucidated, and their relation to parthenogenesis in pig is largely unknown. ResultHere, we reconstructed 189,228 transcripts and identified 7,185 lincRNAs in a single mature oocyte and discovered three stages between in vitro fertilization (IVF) and parthenogenesis activation (PA). A large change in transcriptome occurred in early blastocyst and morula in IVF and PA, respectively, indicating tremendous alteration for the transcriptome in both processes. Compare to protein-coding genes, differential expression protein-coding genes have higher alternative splicing rate, and most transcripts possibly originated from transcription start site (TSS) and transcription terminal site (TTS) types of alternative splicing according to the average expression of 12 AS types. Most lincRNAs with remarkably stage specificity were found in both in procceses. Function prediction results suggest that the specifically expressed lincRNAs in distinct stages have different biological functions. Hub lincRNAs analysis showed that three lincRNAs may play important roles on early embryonic development, and five may function on abnormal parthenogenesis embryonic development. Comparison of the transcriptome between IVF and PA revealed that most DEGs enriched the regulation of apoptotic and late embryonic development-related process. conclusionWe performed a comprehensive comparative analysis framework of RNA-seq data, including mRNA, alternative splicing and long intergenic noncoding RNA. This work identified functional protein-coding genes and lincRNAs, and showed differences in the expression pattern of alternative splicing in IVF and PA. Which detailed the early embryonic development-related procedure in porcine, some protein-coding genes, and lincRNAs that may affect pre-implantation failure in PA embryos. This study will help future research on these genes and molecular-assisted breeding for pig parthenotes.
Common bean resistance to Xanthomonas is associated with upregulation of the salicylic acid pathway and downregulation of photosynthesis
Authors: Foucher Justine , Ruh Mylène , Préveaux Anne , Carrère Sébastien , Pelletier Sandra , Briand Martial , Serre Rémy-Félix , Jacques Marie-Agnès , Chen Nicolas W.G. 
Published: March 2020

Abstract
Background: Common bacterial blight (CBB) caused by Xanthomonas phaseoli pv. phaseoli and Xanthomonas citri pv. fuscans is one of the major threats to common bean crops (Phaseolus vulgaris L.). Resistance to CBB is particularly complex as 26 quantitative resistance loci to CBB have been described so far. To date, transcriptomic studies after CBB infection have been very scarce and the molecular mechanisms underlying susceptibility or resistance are largely unknown. Results: We sequenced and annotated the genomes of two common bean genotypes being either resistant (BAT93) or susceptible (JaloEEP558) to CBB. Reciprocal BLAST analysis led to a list of homologs between these genotypes and the common bean reference genome (G19833), which provides a solid dataset for further comparative analyses. RNA-Seq after inoculation with X. phaseoli pv. phaseoli showed that the susceptible genotype initiated a more intense and diverse biological response than the resistant genotype. Resistance was linked to upregulation of the salicylic acid pathway and downregulation of photosynthesis and sugar metabolism, while susceptibility was linked to downregulation of resistance genes and upregulation of the jasmonic acid/ethylene pathway and of genes involved in cell wall modification.Conclusions: This study helps better understanding the mechanisms occurring during the early colonization phase of common bean by Xanthomonas and unveils new actors potentially important for resistance and susceptibility to CBB. We discuss the potential link between the pathways induced during bean colonization and genes induced by transcription activator-like effectors (TALEs), as illustrated in other Xanthomonas pathovars.