bluuebunny/crossref_metadata_2025_split huggingface dataset:
Social Listening – Revealing Parkinson’s Disease over Day and Night
Authors: Zhang Hui , Meng Fanwen , Li Xingyu , Ning Yali , Cai Meng 
Published: August 2020

Abstract
Background: Nocturnal symptoms in Parkinson’s disease and their related burdens on patients are often treated after management of daytime manifestations. In order to better understand the unmet needs of nocturnal symptoms management, we sought to analyze the characteristics of nocturnal symptoms and their associated burden from patients’ perspectives in 2016 to 2018 and explore whether there were any changes in participants' needs over time across 3 years. Overall symptoms (occurring at day or night) were collected to contrast and compare whether the unmet healthcare needs related to nocturnal symptoms and to overall symptoms are different.Methods: We used a contemporary Social Listening big-data technique to analyze large amounts of Parkinson’s disease symptoms in patient-doctor and patient-patient dialogues available from social media platforms in 2016 to 2018 in China. These symptoms were classified as either overall symptoms or nocturnal symptoms. We used share of voice (SOV) of symptoms as a proportion of total dialogues per year to reflect the characteristics of symptoms from patients’ perspectives. Negative sentiment score of symptoms was analyzed to find out their related burden on patients. Results: We found that SOV for overall motor symptoms was 79% and had not increased between 2016 and 2018 (79%, p = 0.5), but SOV for non-motor symptoms (69% in 2018) had grown by 7% in 2018 (p < 0.01). SOV for motor complications was 9%, and had increased by 6% (p < 0.01) by 2018. The SOV of motor symptoms was significantly larger than non-motor symptoms and motor complications (p < 0.01) in each year. The SOV of non-motor symptoms was larger than that of motor complication (p < 0.01). For nocturnal symptoms, 45% of the analyzed PD population reported nocturnal symptoms in 2018, growing by 6% from 2016 (p < 0.01). SOV for nocturnal-occurring motor symptoms was higher than most non-motor symptoms (except insomnia). However, non-motor symptoms had the higher increases in SOV. Non-motor symptoms evoked higher negative sentiment regardless of whether they occurred during the day or night. For symptoms that can occur at either day or night, each nocturnal symptom was rated with a higher negative sentiment score than the same symptom during the day, regardless of the type of symptom.Conclusions: The growing share of voice and the greater negative sentiment of nocturnal symptoms from patients’ perspectives suggest that management of nocturnal symptoms is an unmet need of patients with Parkinson’s disease. A greater emphasis on detecting nocturnal symptoms and treating them with 24-hour care is encouraged.
Obestatin signaling controls Schwann cells and axonal transport to counteract neuromuscular synaptic loss in skeletal muscle
Authors: Camiña Jesus P , Sánchez-Temprano Agustín , Leal-López Saúl , González-Sánchez Jessica , Mosteiro Carlos S. , Gallego Rosalia , Relova José Luis , Pazos Yolanda 
Published: August 2020

Abstract
Background.

Injuries to the peripheral nerve system are common conditions, with broad spectrum of symptoms depending on the impaired nerves and severity of damage. Although peripheral nervous system retains a remarkable ability for regeneration, it is estimated that less than ten percent of patients fully recover function after nerve injury and the available treatments remain suboptimal. Here, we identify a role for the obestatin/GPR39 system in the regulation of the Schwann cell plasticity as well as in the preservation of neuromuscular synapses in the course of nerve repair.
Methods.

Utilizing a compression model of sciatic nerve injury, axonotmesis, we assessed the obestatin-related regenerative response in the peripheral nerve system. The role of the obestatin/GPR39 system was further evaluated on immortalized rat Schwann cells, IFRS1, and the model of neuronal differentiation, PC12 cells. The interactions between SCs and neurons was evaluated using a co-culture system that combine the SC cell line IFRS1 and the NGF-primed PC12.
Results.

Obestatin signaling directs proliferation and migration of Schwann cells that sustain axonal regrowth and later remyelinate regenerated axons. We provide evidence supporting the preservation of skeletal muscle by the maintenance of neuromuscular synapses through the axonal regulation of calpain-calpastatin proteolytic system. This encompasses the control of skeletal muscle homeostasis by regulation of the ubiquitin proteasome system and the autophagy machinery.
Conclusions.

These results provide important insights into how the obestatin/GPR39 system promotes nerve repair through integration of multiple molecular cues of neuron-Schwann cells crosstalk aimed to promote axon growth and guide axons back to their targets.
Increased dose of re-irradiation therapy or combined chemotherapy to improve survival of local recurrent esophageal squamous cell carcinoma after radiotherapy
Authors: Wu Xun , Hu Xingsheng , Chen Junru , He Lang 
Published: August 2020

Abstract
Introduction: This study aimed to explore the optimal treatment for locally recurrent esophageal squamous cell carcinoma (LRESCC) by analyzing the results of different doses of re-irradiation therapy after radical (chemo) radiotherapy. Methods: We retrospectively analyzed 125 patients with LRESCC after initial radiotherapy. According to radiotherapy dose, 58 patients were assigned to the low-dose (LD) group (50–54 Gy) and 67 to the high-dose (HD) group (55–60 Gy). We recorded the response rate (complete + partial response), 1-, 2- and 3-year survival rates, and toxicity in the two groups. The effects of different radiotherapy doses and combination chemotherapy on the survival of LRESCC were analyzed. Results: After re-irradiation, the 1-, 2- and 3-year survival rates were 48.3%, 24.1% and 10.3% in the LD group and 61.2%, 34.3% and 19.4% in the HD group (P<0.05), respectively. The median survival time of patients receiving radiotherapy alone was 9 months in the LD group and 15 months in the HD group (P<0.05). The survival rate of patients treated with chemoradiotherapy was higher than that of patients treated with radiotherapy alone in the LD group, but in the HD group, chemoradiotherapy showed no advantage compared with radiotherapy alone. The most common toxicity was esophagitis, and the incidence was higher in the HD group than in the LD group (68.7% vs 58.6%, P＜0.05). Conclusion: Increasing the dose of re-irradiation therapy or chemotherapy can improve the efficacy of re-irradiation therapy for patients with LRESCC after radiotherapy.
The Novel Mutations of Unexplained Abortion Were Analyzed at the Nucleotide Level and Classified According to Gene Function
Authors: Yao Ji , Chen Peishan , Zhang Xialan , Liu Wei , Xu Manjuan , Yang Chuanchun , Tang Shanmei , Cui Xiaoli , Chen Chuan , Ou Jian , Tang Huiru , Xu Chengkang 
Published: August 2020

Abstract
Background Abortion is a major problem affecting women's normal reproductive life. Non-whole chromosomes account for about 50% of abortions. In around 50% of cases, the etiology remains unknown. The present analysis was aimed to screen the mutations at the nucleotide level and detected new genetic origins of human diseases. Results 72 abortion tissues (with abnormal chromosome reason excluded) were recruited and whole-genome low coverage mate-pair sequencing (WGL-MPS) was performed on each tissue. And used 100 healthy human data as a background control to find specific relevant genes and mutations with stringent bioinformatics analysis. We merged 72 abortion samples after sequencing. 28 sequence mutations in 25 genes potentially associated with unexplained abortion were identified in abortion. These 25 genes were divided into six classifications, including fetal development, cell cycle, genital correlation, kidneys, nerve, and other know functions. The mutation frequency of these sites was greater than 20% compared with the reference sequence in merged abortion data. The frequency of mutations in the samples of the control group at these sites was less than 1%. Furthermore, we verified by 10 unexplained abortion tissues, seven of which had above mutations sites on these 25 genes.Conclusions We identified 28 mutations in 25 genes mutation that may be associated with unexplained abortion, and some were associated with embryonic development. In conclusion, the results of the present study provide a good research direction for unexplained abortions.
The Prognostic Values of m6A RNA Methylation Regulators in Thyroid Carcinoma
Authors: Zhang Di , Ma Yue , Cao Xu-Chen , Fu Li , Yu Yue , Wang Xin 
Published: August 2020

Abstract
Background: N6-methyladenosine (m6A) is the most common RNA modification and regulates RNA splicing, translation, translocation, and stability. Aberrant expression of m6A has been reported in various types of human cancers. m6A RNA modification is dynamically and reversibly mediated by different regulators, including methyltransferase, demethylases, and m6A binding proteins. However, the role of m6A RNA methylation regulators in thyroid cancer remains unknown. The aim of this study is to investigate the effect of the 13 main m6A RNA modification regulators in thyroid carcinoma.Methods: The gene expression profile of m6A RNA modification regulations and clinical information of patients with thyroid carcinoma were obtained from The Cancer Genome Atlas database. Consensus clustering was applied to identify two clusters of thyroid carcinomas with different clinical outcome. LASSO Cox regression analysis was used to construct gene-based prognostic signature based on the expression of m6A RNA methylation regulators. Kyoto Encyclopedia of Genes and Genomes, Gene Ontology and gene set enrichment analyses were performed to explore differential cellular processes and signaling pathways between the two groups based on risk signature.Results: We found that most of the m6A RNA modification regulators are down-regulated in 450 patients with thyroid carcinoma. We identified two clusters based on the gene expression profiles of 13 m6A RNA modification regulators using consensus clustering. The cluster 2 subgroup correlates with an unfavorable outcome compared with the cluster 1 subgroup. In addition, we derived a three m6A RNA modification regulator genes-based risk signature (FTO, RBM15 and KIAA1429), that is an independent prognostic biomarker in patients with thyroid carcinoma. There were significantly different signaling pathways between high and low risk group by Kyoto Encyclopedia of Genes and Genomes, Gene Ontology and gene set enrichment analyses. Moreover, we found that this risk signature could better predict outcome in male than female. Conclusion: Our study revealed the prognostic value of m6A RNA methylation regulators in patients with thyroid carcinoma.
Secure Transmission in Cognitive Radio Networks using Full-duplex Technique with Outdated CSI
Authors: Shang Zhihui , Zhang Tao , Wu Hao , Qiao Xiaoqiang , Tao Liwei 
Published: August 2020

Abstract
In this paper, we investigate the effects of the outdated channel state information (CSI) on the secrecy performance of an underlay spectrum sharing cognitive radio networks (CRNs) with full-duplex (FD) receivers. Legitimate destination node (Bob) acts as a FD node, transmitting interference signals to a passive eavesdropping node (Eve) while receiving signals. To exploit the benefits of collaborative zero-forcing beamforming and FD technologies, transmit antenna selection-maximal-ratio combining-zero forcing beamforming/maximal-ratio transmission-selection combining-zero forcing beamforming (TAS-MRC-ZFB/MRT-SC-ZFB) scheme is considered at the secondary user transmission link with the outdated CSI and collaborative ZFB
is adopted at FD destination node, which tries to improve the transmission efficiency of our considered CRNs. Specifically, in order to evaluate the proposed scheme, the derivation of analytical expression in exact and the high asymptotic closed-form expressions of secrecy outage probability (SOP) are provided of the considered network with and without feedback delay of the CSI. Furthermore, the impact of delay on the second user transmission and interference links of the primary user (PU) node is investigated. The results reveal that outdated CSI of secondary user transmission and collaborative ZFB channels deteriorates the SOP, and the outdated CSI between PU and SU will affect the power margin factors for the secondary transmitter. Furthermore, we also study the effects of different system parameters on the secrecy diversity order and secrecy array gain. The proposed scheme is compared with the existing TAS-MRC/TAS-MRC scheme to verify the improvement of system performance. Finally, Monte Carlo simulations verify the correctness of our theoretical analysis.
Enhancement of SARS-CoV-2 Receptor Binding Domain -CR3022 Human Antibody Binding Affinity via in Silico Engineering Approach
Authors: Sefid Fateme , Payandeh Zahra , Azamirad Ghasem , Mansoori Behzad , Baradaran Behzad , Sadeghi Mohammad Reza 
Published: August 2020

Abstract
Background: The nCoV-2019 is a cause of COVID-19 disease. The surface spike glycoprotein (S), which is necessary for virus entry through the intervention of the host receptor and it mediates virus-host membrane fusion, is the primary coronavirus antigen (Ag). The angiotensin-converting enzyme 2 (ACE2) is reported to be the effective human receptor for SARS-CoVs 2. ACE2 receptor can be prevented by neutralizing antibodies (nAbs) such as CR3022 targeting the virus receptor-binding site. Considering the importance of computational docking, and affinity maturation we aimed to find the important amino acids of the CR3022 antibody (Ab). These amino acids were then replaced by other amino acids to improve Ab-binding affinity to a receptor-binding domain (RBD) of the 2019-nCoV spike protein. Finally, we measured the binding affinity of Ab variants to the Ag. Result: Our findings disclosed that several variant mutations could successfully improve the characteristics of the Ab binding compared to the normal antibodies. Conclusion: The modified antibodies may be possible candidates for stronger affinity binding to Ags which in turn can affect the specificity and sensitivity of antibodies.
Improvements in the Degree of Understanding the Treatment Guidelines for Schizophrenia and Major Depressive Disorder in a Nationwide Dissemination and Implementation Study
Authors: Numata Shusuke , Nakataki Masahito , Hasegawa Naomi , Takaesu Yoshikazu , Takeshima Masahiro , Onitsuka Toshiaki , Nakamura Toshinori , Edagawa Reon , Edo Hiroaki , Miura Kenichiro , Matsumoto Junya , Yasui-Furukori Norio , Kishimoto Taishiro , Hori Hikaru , Tsuboi Takashi , Yasuda Yuka , Furihata Ryuji , Muraoka Hiroyuki , Ochi Shinichiro , Nagasawa Tatsuya , Kyou Toshitaka , Murata Atsunobu , Katsumoto Eiichi , Ohi Kazutaka , Hishimoto Akitoyo , Inada Ken , Watanabe Koichiro , Hashimoto Ryota 
Published: August 2020

Abstract
Background: To implement clinical practice guidelines (CPGs), it is necessary for psychiatrists to deepen their understanding of the CPGs. The Effectiveness of Guidelines for Dissemination and Education in Psychiatric Treatment (EGUIDE) project is a nationwide dissemination and implementation study of two sets of CPGs for schizophrenia and major depressive disorder (MDD).Methods: A total of 413 psychiatrists (n=212 in 2016; n=201 in 2017) learned the two CPGs in the education program of the EGUIDE project over two days, and clinical knowledge of the two CPGs was evaluated by two 37-item questionnaires at baseline and after the EGUIDE programs. To improve the correct answer rate for clinical knowledge after the EGUIDE programs, we revised the lecture materials associated with items that had a low correct answer rate in 2016 and used the revised lecture materials with the CPGs in 2017. The rates of correct answers after the EGUIDE programs for each item between the 2016 and 2017 groups were compared.Results: The total clinical knowledge scores after the EGUIDE program significantly increased in both the 2016 groups and the 2017 groups. We revised lecture materials related to two items in the schizophrenia CPG and five items in the MDD CPG that had low correct answer rates after the EGUIDE programs. The correct answer rate of one item on the schizophrenia CPG and one item on the MDD CPG tended to be improved (S-D5, p=0.038; D-C6, p=0.033) and that of one on the MDD CPG was significantly improved (D-D3, p=0.00080) in the 2017 group compared to those in the 2016 group. The other four items were not significantly improved.Conclusions: We reported improvements in clinical knowledge of CPGs after the EGUIDE program in the 2017 group following revision of the lecture materials based on results from the 2016 group. These attempts to improve the degree of understanding of CPGs may facilitate the successful dissemination and implementation of psychiatric guidelines in everyday practice.Trial registration: Effectiveness of Guideline for Dissemination and Education in Psychiatric Treatment, UMIN000022645, Registered 15 June 2016, https://upload.umin.ac.jp/cgi-open-bin/ctr/ctr.cgi?function=brows&action=brows&recptno=R000026044&type=summary&language=J
Recognizing the waveform of a foreshock
Authors: Lippiello Eugenio , Petrillo Giuseppe , Godano Cataldo 
Published: August 2020

Abstract
The 2011 Mw9.1 Tohoku, Japan, earthquake is the paradigmatic example of an earthquake anticipated by a significant foreshock activity, with a Mw7.3 earthquake occurred two days before, within about 10 km 1. Recent results 2 show that statistically relevant changes can be found in the magnitude distribution after the Mw7.3 foreshock but the discrimination between normal and foreshock activity still remains a scientific challenge 3. Here we show that the envelope of the ground velocity recorded after the Mw7.3 foreshock presents an atypical sawtooth profile very different from the one observed after other earthquakes 4. We interpret this profile as the consequence of the locked state of the mainshock fault which reduces the possibility of the foreshock to trigger its own aftershocks. We find a similar sawtooth profile after other Mw6+ foreshocks followed within 10 days by a larger earthquake, as in the case of the 2014 Mw8.1 Iquique, Chile, sequence. This observation allows us to define a level of concern, simply extracted from the first 45 minutes of the recording waveform, associated to the occurrence of a larger earthquake. A test of the method for 47 Mw6+ worldwide earthquakes gives precise warning in time and space after all the 10 earthquakes followed by a larger one with only 2 false alerts.
WITHDRAWN: Correlation between DVH parameters and lung function changes before and after radiotherapy and the occurrence of radiation-induced lung injury (RILI)
Authors: Sha Sha 
Published: August 2020

Abstract
Background: The purpose of the study was to explore the correlation between DVH parameters and lung function changes before and after radiotherapy and the occurrence of radiation-induced lung injury (RILI), and to evaluate its value in predicting the risk of RILI.Methods: 120 patients with advanced non-small cell lung cancer who had been diagnosed in Jiaozhou Central Hospital of Qingdao City in the past three years and received chest conformal (intensity modulated) radiation therapy were selected. Before radiotherapy, irradiation of 45-50 Gy, and 1 month after the end of radiotherapy, the patients were tested for lung function, and the ventilation and diffusion function of the patients were tested using the Japanese CHESTAC-8800 lung function tester. The evaluation of radiation lung injury was based on the RTOG acute radiation lung injury classification standard, and the observation end point was ≥2 grade RILI.Results: A total of 34 patients with ≥2 grade RILI among 120 patients in this study, including 23 cases of grade 2 and 11 cases of grade 3, the incidence rate was 28.33%. The difference between FVC, FEV1, FEV1 / FVC, DLCO, V5, V10, V15 before radiotherapy, 45-50 Gy, and 1 month after the end of radiotherapy were statistically significant (P <0.05). Univariate analysis showed that lung function, V5, V10, and V15 before radiotherapy were related factors for RILI (P <0.05). Multivariate logistic analysis showed that the risk of RILI was 1.855 times that of patients with higher FEV1 / FVC before radiation therapy (OR = 1.855, 95% CI = 1.199-1.946, P = 0.037), patients with V10 ≥50% were 3.673 times higher than patients with V10 <50% (OR = 3.673, 95% CI = 1.548-7.582, P = 0.039).Conclusions: V10≥50% and FEV1 / FVC are high-risk factors for RILI before radiotherapy, which has certain value in predicting the risk of RILI.
Blood indices and circulating tumor cells for predicting metastasis of hepatocellular carcinoma after liver transplantation
Authors: Zhuang Li , Liu Xiang-yan , Zhu Heng-kai , Wang Zhuo-yi , Zhang Wu , Jiang Guo-ping , Zheng Shu-sen 
Published: August 2020

Abstract
Background: Liver transplantation (LT) can benefit the long-term survival of hepatocellular carcinoma (HCC) patients. We hypothesized that circulating tumor cell (CTC) levels and subtypes are intimately associated with metastasis status of HCC patients, and this study was designed to test that compositive hematological indices including CTC can provide sensitive and accurate prediction of post-LT metastasis. Methods: Between 2017 and 2018, HCC patients receiving LT were included for analysis. The 24-month follow-up was mainly conducted by outpatient and telephone. Blood samples were collected, and hematological indices were examined. The outcomes such as PFS, recurrence, metastasis, location of recurrence/metastasis, and number of metastases were recorded. Results: The follow-up analysis showed that microvascular invasion (MVI) classification at the baseline is associated with metastasis. Next, AFP level was another useful indicator of postoperative metastasis, especially at the third or fourth month; the PIVKA-II level 3 months after LT was significantly higher for those who had later metastasis. The mesenchymal CTC level at the 45th day was increased for in the metastasis group. Using two-ends Logistic regression, the calculated value MP (metastasis predictor, by above factors). Had an AUC of 0.858 in the ROC curve, with a cutoff value of 0.328. In conclusion, microvascular invasion, AFP level at the third or fourth month, PIVKA-II level at the third month, and mesenchymal CTC level at day 45 were associated with post-LT metastasis. Conclusion: Using Logistic regression based on above variables, the 2-year metastasis can be predicted with satisfactory sensitivity and accuracy.