bluuebunny/crossref_metadata_2025_split huggingface dataset:
Morphological Markers of Pathological Changes in Bitches Endometrium Affected by a Cystic Endometrial Hyperplasia - Pyometra Complex (CEH-P)
Authors: Woźna-Wysocka Magdalena , Rybska Marta , Błaszak Beata , Jaśkowski Bartłomiej Maria , Kulus Magdalena , Jaśkowski Jędrzej Maria 
Published: May 2020

Abstract
Background The aim of the analysis was to designate the morphological symptoms that appear during the Cystic Endometrial Hyperplasia - Pyometra complex (CEH-P), diagnosed by microscopic examination. The investigation were conducted on the uteri of 120 bitches in age between 1–16. The microscopic examinations were based on histological stainings. The aim of examinations was to find the differences in morphology of endometrium in the specimens of the uteri wall (H&E stain). All of the uteri were divided into three pathological groups (GI – GIII), created on the basis of clinically symptoms of analyzed diseases. Uteri without symptoms were classified as a control group (C).Results Histopathological analysis that were conducted have confirmed preliminary macroscopic evaluation for control group with unchanged uteri, group GII with cystic endometrial hyperplasia of uteri (CEH), and group GIII with uteri with pyometra. The confirmation of compatibility of both macroscopic and microscopic evaluation of the uteri were observed in groups GII and GIII. In the uteri of the group GI a severe congestion of endometrium have been observed – it is typical for the inflammation – which was not confirmed during histopathological examinations. Those examinations revealed only endometrial haemorrhage.Conclusions The results are showing that during classification of research material microscopic examinations are required. The diagnose based on the macroscopic changes in typical for CEH-P symptoms might be incorrect, if it is not supported by detailed research. More than that, in all uteri with the closed pyometra the CEH was also observed. It suggest that pyometra may occur as a consequence of cystic endometrial hyperplasia and bacterial infections ended with inflammation. The results obtained can be used to create a basis for pathologic classification of endometrial hyperplasia, including CEH-P complex in bitches.
Identification and Verification of Candidate Genes Regulating Human Umbilical Vein Endothelial Cells Behavior Under Hyperglycemia
Authors: MA XIAOYE , ZHOU YUCHEN , XIE JUNCHAO , MENG GUILIN , ZHAO YICHEN , ZHAO YANXIN , Liu Xueyuan 
Published: May 2020

Abstract
Background: Diabetes is a metabolic disease that has been widely demonstrated to be correlated with many microvascular and macrovascular diseases that seriously damage the patient’s life quality. This study intended to investigate the underlying molecular mechanisms of endothelial dysfunction under hyperglycemia. Methods: The gene expression profile of GSE49524 was downloaded and differentially expressed genes (DEGs) in hyperglycemia human umbilical cord endothelial cells (HUVECs) samples compared with normoglycemia HUVECs samples were identified by R software. Afterward, we analyzed the data by applying a combination of the bioinformatics method and used the microRNAs (miRNAs) databases to predict microRNAs that target key genes. The expression of the top 10 differentially expressed genes was validated through quantitative real-time PCR (qRT-PCR). Results: A total of 65 genes were distinguished as DEGs. The dominant GO (gene ontology) terms and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathways which were significantly overrepresented in the hyperglycemia HUVECs were identified. The results of the protein-protein interaction networks demonstrated that fibronectin 1 (FN1) is of the highest degree. In addition, several predicted miRNAs that target FN1 were obtained too. The further verification of the top 10 DEGs through qRT-PCR illustrated that nine of the up-regulated DEGs were up-regulated significantly in the hyperglycemia group when compared to the control group. Conclusions:This exploratory study may help to prompt an understanding of the underlying molecular mechanisms of the effect of hyperglycemia on the behavior of HUVECs and contribute to the production of effective therapeutic interventions.
Let-7d inhibits intratumoral macrophage M2 polarization and subsequent tumor angiogenesis by targeting IL-13 and IL-10
Authors: Su Boxing , Han Haibo , Gong Yanqing , Li Xuesong , Ji Chaoyue , Yao Jingjing , Yang Jianghui , Hu Weiguo , Zhao Wei , Li Jianxing , Zhang Gang , Zhou Liqun 
Published: May 2020

Abstract
Background: The microRNA let-7d has been reported to be a tumor suppressor in many types of cancer, including renal cell carcinoma (RCC). Tumor-associated macrophages are M2-polarized macrophages that can enhance tumor growth and angiogenesis in many human cancers. However, the role of let-7d in TAM-associated RCC tumor progression remains elusive.Methods: Human macrophage cell line THP-1 cells pretreated with phorbol myristate acetate were co-cultured with let-7d overexpressing RCC cells, the conditioned medium were obtained and its effect on human umbilical vein endothelial cells (HUVECs) was accessed in vitro. The phenotype of cocultured macrophages and macrophages in mouse models bearing let-7d overexpressing RCC cell derived xenograft were analyzed in vitro and in vivo using flow cytometry and quantitative real-time PCR.Results: First, we observed a strongly inverse correlation between let-7d expression and microvessel density in RCC tissues. Furthermore, the proliferation, migration, and tube formation of HUVECs was significantly inhibited by conditioned medium from a coculture system of the human macrophage THP-1 cell line with let-7d-overexpressing RCC cells. Moreover, the proportion of M2 macrophages was significantly lower in the group that was cocultured with let-7d-overexpressing RCC cells. Subcutaneous xenografts formed by the injection of let-7d-overexpressing RCC cells together with THP-1 cells resulted in a significant decrease in the M2 macrophage ratio and microvessel density compared with those formed by the injection of control RCC cells with THP-1 cells. In silico analysis revealed interleukin-10 (IL-10) and IL-13 as potential let-7d target genes. Consistently, the activities of the luciferase reporters containing the 3’-UTRs of the IL-10 and IL-13 genes were significantly inhibited by let-7d. Importantly, the addition of IL-10 and IL-13 counteracted the inhibitory effects of the conditioned medium from the coculture system of let-7d-overexpressing RCC cells in vitro. Additionally, the overexpression of IL-10 and IL-13 reversed the effects of let-7d on macrophage M2 polarization and tumor angiogenesis in vivo. Finally, the expression of IL-10 and IL-13 were inversely correlated with the expression of let-7d in RCC clinical specimens.Conclusions: These results suggest that let-7d may inhibit intratumoral macrophage M2 polarization and subsequent tumor angiogenesis at least in part by targeting IL-10 and IL-13.
Modelling of Seismicity in Southern Pakistan using GIS Techniques
Authors: Khan Muhammad 
Published: May 2020

Abstract
This study was aimed to expound spatial association between tectonic earthquakes and individual faults in southern Pakistan. A population of fault lines are exposed to the onshore region located to the north of active Makran subduction zone and within Indus Basin where geophysical prospecting reported neotectonic deformation and faulting. A catalog of earthquakes has been compiled considering the seismic events originated from southern Pakistan and neighboring southeastern Iran, southern Afghanistan and frontal offshore areas. Since, the faulty blocks are extending beyond the international borders, a wide region was outlined for cataloging and thorough screening of fault lines. The catalog has been thoroughly processed by standard procedures necessary for magnitude coherency, main shocks decluttering, magnitude completeness and removal of spatially outlier events etc. GIS-based vectors of fault lines were abstracted by the digitization of regional tectonic lineaments and structural maps. A rigorous effort was made to review available literature to gather updated data of plate kinematics, GPS constraints, relative blocks movement across the faults and rate of fault mechanics (slip-sense) during major events. The weights of evidence method was used to judge the spatial associations between seismic events and populations of faults lines to ascribe ‘seismicity index’ which ranked the potential faults from I (least active level) to V (highly active level). The SI helped to compare each fault response e.g. seismicity yield, released seismic stresses collinear to the fault line, active or aseismic segments and probabilistic estimate for future sizeable earthquake. Kernel density maps were prepared to allure the vulnerable pockets of the elongated faults for specific magnitude windows. The spatial patterns of earthquakes in southern Pakistan were significant to study the seismogenic potential associated with the active tectonic lineaments and faulted blocks. Spatial analysis of data sets for seismic events suggest stronger spatial associations between earthquake epicenters and faults oriented N-S, and weaker spatial associations of epicenters with some -unknown faults in the plains of Indus Basin.
The Effect of Bone Cement Distribution on the Outcome of Percutaneous Vertebroplasty: A Case Cohort Study
Authors: Tan Lei , Wen Bingtao , Guo Zhaoqing , Chen Zhongqiang 
Published: May 2020

Abstract
Background: To analyze the effect of different types of bone cement distribution after percutaneous vertebroplasty (PVP) in patients with osteoporotic vertebral compression fracture (OVCF). Methods: 137 patients withsinglelevelOVCF who underwent PVP were retrospectively analyzed. The patients were divided into two groups according to bone cementdistribution. Group A:bone cement contacted both upper and lower endplates; Group B: bone cement missed at least one endplate. Group Bwas divided into 3 subgroups. Group B1: bone cement only contacted the upper endplates; Group B2: bone cement only contacted the lower endplates; Group B3: bone cement only located in the middle of vertebral body. The visual analogue scale (VAS) scoreat 24 hours post operation and last follow-up, anterior vertebral height restoration ratio (AVHRR), anterior vertebral height loss ratio (AVHLR) and vertebral body recompression ratewere compared. Results: 24 hours post operation, the pain of all groups were significantly improved. At the last follow-up, the VAS score of group A was lower than that of group B. There were 9 cases (6.6%) of cement leakage, 4 cases (6.9%) in group A and 5 cases (6.3%) in group B. At the last follow-up, there were 16 cases (11.7%) of vertebral body recompression, including 3 cases (5.2%) in group A and 13 cases (16.5%) in group B. There was no significant difference in AVHRR between two groups. At the last follow-up, AVHLR in group B was higher than that in group A. In subgroup analysis, there was no significant difference in VAS score,vertebral recompression rate, AVHRR or AVHLR.Conclusions: If the bone cement fully contacted both the upper and lower endplates, it can better restore the strength of the vertebral bodyand maintain the height of the vertebral body, reduce the risk of the vertebral body recompression and long term pain.
Mechanism of lncRNA H19 in the Regulation of Lipid Accumulation and Inflammatory Response in Macrophages
Authors: Wang Xue-Mei , Gao Xiao-Ming , Liu Fen , Cao Ying , Wang Jie-Ying , Xing Yuan 
Published: May 2020

Abstract
Background and aims: Lipid accumulation of macrophages caused by oxidative stress is the key reason for the early pathological changes of atherosclerosis. LncRNA H19 repression downregulated NF-κB activation, upregulated ABCA1 expression, intracellular lipid accumulation increased, but the role of lncRNA H19 in atherogenesis and the molecular mechanisms have not been defined. We aimed to explore if and how lncRNA H19 affects lipid accumulation of macrophages by regulating lipid metabolism and inflammatory response.Methods and results: THP-1 macrophages were cultured with ox-LDL to form foam cells. THP-1-derived macrophages were incubated with H19 siRNA or not. Oil Red O staining was used for the determination lipid accumulation in macrophages. Enzymatic methods were performed to analyze cholesterol concentration. Both western blot and qRT-PCR were applied to detect target gene expression. ELISA was used to examine the levels of oxidative and inflammatory mediators. We found that lncRNA H19 repression reduced lipid accumulation by elevating efficiency of RCT and via upregulation of ABCA1 and PPARα expression in THP-1 derived macrophages. Further, lncRNA H19 repression upregulated PGC-1α and downregulated NF-κB signaling pathway.Conclusion: These results suggest that lncRNA H19 repression inhibits atherosclerosis by promoting RCT process and reducing inflammatory response via PGC-1α and NF-κB pathways, respectively.
Mesangial IgM Deposition predicts renal outcome in patients with IgA nephropathy: A multicenter, prospective, observational study
Authors: Tan Li , Tang Yi , Pei Gai-Qin , Zhong Zheng-Xia , Tan Jia-Xing , Ma Ya , Wang Dong-Guang , Zhou Ling , Sheikh-Hamad David , Qin Wei 
Published: May 2020

Abstract
BackgroundMesangial IgM deposition is commonly found in patients with immunoglobulin A nephropathy (IgAN). This study aims to investigate the relationships between mesangial IgM deposition and disease progression in IgAN patients.MethodsA total of 1239 patients with biopsy-proven primary IgAN were enrolled in this multicenter, prospective, observational study between January 2013 and August 2017. According to the degree of IgM deposition, 1239 patients were divided into three groups: Grade 0 (no or trace; n = 713, 57.55%), Grade 1 (mild; n = 414, 33.41%), Grades 2 + 3 (moderate and marked; n = 112, 9.04%). Using a 1: 1 propensity score matching (PSM) method identifying age, gender, and treatment modality to minimize confounding factors, 1042 matched patients (out of 1239) with different degrees of IgM deposition were enrolled to evaluate the severity of baseline clinicopathological features and renal outcome: Grade 0 (n = 521, 50.00%), Grade 1 (n = 409, 39.25%), Grades 2 + 3 (n = 112, 10.75%). Kaplan–Meier and Cox proportional hazards analyses were performed to determine whether different degrees of mesangial IgM deposition are associated with varying renal outcomes in IgAN.ResultsDuring a mean follow-up of 48.90 ± 23.86 and 49.01 ± 23.73 months, before and after adjusting for propensity scores respectively, the rate of complete remission (CR) was progressively lower with increased IgM deposition in both unmatched (63.39%, 46.14%, 45.54%) and matched cohort (61.80%, 46.45%, 45.54%), whereas the proportion of patients progressing to end stage renal disease (ESRD) showed reverse correlation (P < 0.001). Kaplan–Meier analysis indicated negative correlation between the intensity of mesangial IgM deposits and cumulative renal survival (all P < 0.05). Moreover, Cox regression analysis revealed that the degree of mesangial IgM deposition predicted renal outcome independent of MESTC score and clinical variables in the unmatched (Grade 1, HR, 1.59; 95% CI, 1.11–2.29; P = 0.01; Grades 2 + 3, HR, 1.69; 95% CI, 1.02–2.08; P = 0.04) and matched cohort (Grade 1, HR, 1.84; 95% CI, 1.19–2.85; P = 0.01; Grades 2 + 3, HR, 1.91; 95% CI, 1.01–3.24; P = 0.04).ConclusionsMesangial IgM deposition plays an important role in renal prognosis and is independently associated with worse renal outcomes in patients with IgAN.Trial registration TCTR, TCTR20140515001. Registered May 15, 2014, http://www.clinicaltrials.in.th/index.php?tp=regtrials&menu=trialsearch&smenu=fulltext&task=search&task2=view1&id=1074
Identification of Protective Peptides of Fasciola Hepatica-Derived Cathepsin L1 (FhCL1) in Vaccinated Sheep by a Linear B-cell Epitope Mapping Approach
Authors: Perazzo Leandro Buffoni , Garza-Cuartero Laura , Pérez-Caballero Raúl , Zafra Rafael , Martínez-Moreno F. Javier , Molina-Hernández Verónica , Pérez José , Martínez-Moreno Álvaro , Mulcahy Grace 
Published: May 2020

Abstract
The authors have withdrawn this preprint due to erroneous posting.
Dual-source CT in Diagnosis of Coronary Artery Fistula and its Value
Authors: WANG Cheng , LI Yaying , LIU Jiangyong 
Published: May 2020

Abstract
Background: Coronary artery fistula refers to the symptom that the coronary artery trunk or branch connects with the heart cavity or the pulmonary circulation blood vessels in a congenital or an acquired manner. As early detection and diagnosis of CAF was necessary for the prevention and management of late symptoms and complications, we are supposed to master the imaging performance of CAFs so as to make a correct diagnosis. This paper seeks to investigate the imaging manifestations and application value of CT diagnosis of coronary fistula and its application value. Methods: To retrospectively analyze the clinical data and DSCT image data of 62 patients with coronary fistula, and summarize, analyze and summarize their image manifestations and types.Results: 62 CAFs originate from 58 LCA vessels, 32 RCA vessels. The distribution frequency of drainage site from high to low was as follows: 40 cases (63%) of the main pulmonary artery, 10 cases (16%) of the right atrium, 7 cases (11%) of the right ventricle, 3 cases (5%) of the left ventricle, 1 case (2%) of the left atrium, and 1 case of coronary sinus (2%), 1 case of the right lower pulmonary vein (2%). There were 39 cases (63%) of single fistula vessel, and 23 cases (37%) of multiple fistula vessel, including 21 cases of left and right coronary arteries. Coronary artery-pulmonary fistulae were the most common with a total of 40 cases. Conclusion: The popularization of CT improved the detection rate of CAFs. Coronary pulmonary-artery fistula was probably the most common seen type of CAFs. ECG-gating coronary artery CTA can accurately assess the origin of CAFs, the course of fistula vessels, and the drainage site, thereby providing important information for clinicians to treat coronary fistula.Trial registration: retrospectively registered
The Effect of Acromioplasty or Bursectomy on the Results of Arthroscopic Repair of Full Thickness Rotator Cuff Tears: Does the Acromion Type Affect These Results?
Authors: Karakus Ozgun , Sari Ahmet Sinan 
Published: May 2020

Abstract
Introduction: The aim of this study was to investigate the effect of subacromial decompression on the results of full thickness rotator cuff repair applied arthroscopically. Examination was also made of the effect of acromion type on the subacromial decompression procedure in patients applied with arthroscopic rotator cuff repair. Materials and Methods: The study included a total of 150 patients, comprising 102 (68%) females and 48 (32%) males with a full thickness rotator cuff tear repaired arthroscopically. The patients were separated into 3 groups of 50. Group A comprised those with acromioplasty and bursectomy applied additional to the repair. In Group B, only bursectomy was performed additional to the repair and in Group C, only rotator cuff repair was applied. Evaluation was made of the postoperative long-term pain and functional results. Results: The mean age of the cases was 65.63±9.22 years (range, 46-86 years). The affected side was right side in 95 (63.3%) cases and left side in 55 (36.7%). No statistically significant difference was determined between the groups according to the postoperative Constant Murley and ASES scores (p>0.05). In the paired comparisons, the postoperative VAS scores of Group C were higher than those of Groups A and B (p=0.018, p=0.029, p<0.05). No statistically significant difference was determined between Group A and Group B in respect of the postoperative VAS scores (p>0.05). Conclusions: In the arthroscopic repair of full thickness rotator cuff tears, neither acromioplasty, coraco-acromial ligament loosening nor bursectomy were determined to have any positive effect on the results. Whatever the acromion type, there is no need for an additional subacromial decompression procedure after rotator cuff repair, in respect of pain and functional outcomes. Only acromial spurs should be gently removed paying attention to the coraco-acromial ligament.
Geriatric Assessment for Older Adults with Sickle Cell Disease: protocol for a prospective cohort pilot study
Authors: Oyedeji Charity I , Hall Katherine , Luciano Alison , Morey Miriam , Strouse John Joseph 
Published: May 2020

Abstract
Background The life expectancy for people with sickle cell disease (SCD) has improved tremendously over the last 50 years. This population experiences hemolysis and vaso-occlusion in multiple organs that lead to complications such as cardiopulmonary disease, strokes, and avascular necrosis. These complications can limit mobility and aerobic endurance, similar to limitations that often occur in geriatric populations. These sickle-cell and age-related events lead to frequent hospitalization, which further increases the risk of functional decline. We have few tools to measure functional decline in people with SCD. The purpose of this paper is to describe a protocol to evaluate the feasibility of sickle cell disease geriatric assessment (SCD-GA).Methods/Design Forty adults with SCD (20 age 18–49 years and 20 age ≥ 50 years) will be enrolled in a prospective cohort study to assess the feasibility of SCD-GA. We used a validated oncology geriatric assessment and enriched with additional physical and cognitive measures. The SCD-GA will be performed at the first study visit, 10 to 20 days after hospitalization, and at after 12-months (exit visit). With input from a multidisciplinary team of sickle cell specialists, geriatricians, and experts in physical function and physical activity, we selected assessments across 7 domains: functional status (11 measures), comorbid medical conditions (1 measure), psychological state (1 measure), social support (2 measures), weight status (2 measures), cognition (3 measures), and medications (1 measure). We will measure the proportion completing the assessment with feasibility as the primary outcome. Secondary outcomes include the proportion consenting and completing all study visits, duration of the assessment, acceptability, and adverse events.Discussion We present the protocol and rationale for selection of the measures included in SCD-GA. We also outline the methods to determine feasibility and subsequently to optimize the SCD-GA in preparation for a larger multicenter validation study of the SCD-GA.