bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical Features of Pregnant Women and Neonates with COVID-19 in Wuhan: a paired comparison study
Authors: Xie Qin , Gan Quan , Hu Jing , Zhou Dong , Cai Shuhan , Su Lianjiu , Li Yunxiang , Li Yiming , Peng Zhiyong 
Published: June 2020

Abstract
Background

Our study aimed to find symptoms unique in pregnancy and to help the early diagnosis in pregnant women and to evaluate the impact of COVID-19 in postpartum women and their newborns.
Methods

Clinical data were reviewed and collected for 11 pregnant women with laboratory-confirmed COVID-19 who were consecutively admitted to Zhongnan Hospital of Wuhan University and Women and Children’s Hospital of Hubei Province, from Jan 26 to Feb 26, 2020.
Results

All the confirmed women didn’t have any exposure history and their early symptoms were mildly elevated temperate and fatigue. The chest CT scans of confirmed women can be atypical manifestations, such as bilateral pleural effusions and slightly increased densities. Eight of eleven confirmed women did not feel anything unusual until abnormalities were found on chest CT scans on admission screening test. All three groups had elevated white blood cell count, neutrophil count and lactate dehydrogenase, and reduced total protein.The infection did not increase the risk for premature delivery, premature rupture of membrane, or comorbidities in pregnancy.
Conclusions

Pregnant women were often asymptomatic and accidentally detected abnormalities on chest CT scan on admission which emphasize the importance of CT scan in prevalent areas of the COVID-19. Even after the laboratory confirmation, the manifestation of the CT scan could be atypical, which alerted the necessity of protection for healthcare workers. The COVID-19 did not increase the risk of complications in pregnant women and their neonates.
Trial registration:

This case series was approved by the institutional ethics board of Zhongnan Hospital of Wuhan University (No. 2020020) and Women and Children’s Hospital of Hubei Province(NO. LW035).
Reduced-dose of Posttransplant Cyclophosphamide and Cotransplantation of Peripheral Blood Stem Cells and Human Umbilical Cord-derived Mesenchymal Stem Cells in Severe Aplastic Anemia
Authors: Zu Yingling , Zhou Jian , Fu Yuewen , Fang Baijun , Liu Xinjian , Zhang Yanli , Yu Fengkuan , Zuo Wenli , Zhou Hu , Gui Ruirui , Li Zhen , Liu Yanyan , Zhao Huifang , Zhang Chengjuan , Song Yongping 
Published: June 2020

Abstract
Background Posttransplant cyclophosphamide (PTCY) as graft-versus-host disease (GVHD) prophylaxis is an effective strategie for patients receiving matched sibling donor hematopoietic stem cell transplantation (MSD-HSCT) and haploidentical HSCT (haplo-HSCT). Methods We evaluated the effectiveness and safety of reduced-dose cyclophosphamide, 20 mg/kg for 13 patients in the MSD-HSCT group and 25 mg/kg for 22 patients in the haplo-HSCT group, on days +3 and +4 combined with cotransplantation of peripheral blood stem cells (PBSCs) and human umbilical cord-derived mesenchymal stem cells (UC-MSCs) for patients with severe aplastic anemia (SAA) retrospectively. Results In the MSD-PTCY group, the times to neutrophil and platelet engraftment were significantly shorter than those in the MSD-control group (P<0.05), 11 (range 10 to 14) days and 12 (range 9 to 15) days. The cumulative incidence of acute GVHD (aGVHD) at day +100 (15.4%) was lower in the MSD-PTCY group than in the MSD-control group(P=0.050). No patient developed chronic GVHD (cGVHD). The 1-year overall survival (OS) and event-free survival (EFS) rates in the MSD-PTCY group were 100% and 92.3%. In the haplo-PTCY group, the times to neutrophil and platelet engraftment were significantly shorter than those in the haplo-control group (P<0.05), 12 (range 9 to 15) days and 11.5 (range 9 to 17) days. The cumulative incidences of aGVHD at day +100 and 1-year cGVHD in the haplo-PTCY group were 31.8% and 18.2%. The 1-year OS and EFS rates in the haplo-PTCY group were 81.8% and 66.9%. Conclusions Reduced-dose PTCY and cotransplantation of PBSCs and UC-MSCs is feasible for patients with SAA, especially for overcoming the high incidences of aGVHD and cGVHD due to PBSCs.
An Investigation of Construct Validity and Responsiveness of the Danish ICECAP-A
Authors: Holst-Kristensen Annette Willemoes , Mitchell Paul Mark , Linton Myles-Jay , Coast Joanna , Fonager Kirsten , Pedersen Kjeld Møller 
Published: June 2020

Abstract
BackgroundThis study aimed to provide the first assessment of construct validity of ICECAP-A in patients with cardiovascular disease, chronic obstructive pulmonary disease and diabetes, and to assess the responsiveness of the measure in this group. MethodData were provided from patients attending rehabilitation in the municipality of Aalborg, Denmark, from March 2018 to March 2019. Patients answered a questionnaire from the healthcare centre and the ICECAP-A at baseline and 12 weeks follow-up. To assess construct validity, a priori hypotheses were developed. Based on these hypotheses, associations between sociodemographic characteristics, ‘general health’, a freedom dimension, and ICECAP-A were analysed through chi-squared tests and Spearman rank correlations for categorical and ordinal variables, respectively. To investigate responsiveness, the anchor-based method was used. Patients were divided into improved, worsened or no change, based on changes between baseline and follow-up on the anchor measures (‘general health’ and ‘freedom’). To quantify responsiveness, both the weighted and un-weighted ICECAP-A scores’ effect sizes, standardised response means and t-tests were used. Findings were explored across different age groups.ResultOf all the hypothesised associations, 16 of 26 (62%) were in the expected direction. The expected relationships were found between ICECAP-A scores and general health and freedom to do things. ICECAP-A was responsive in terms of capturing the effects on general health and the freedom to do things. Differences were found across age groups, with greater responsiveness to change in those aged under 65 years. The item-by-item analysis showed that capability was mainly driven by stability and autonomy.ConclusionThis study has shown that the Danish ICECAP-A is a valid and responsive measure of the effects of an exercise and education-based rehabilitation programme.
Temporal Heterogeneity of ROS1 Fusion and Braf Fusion Conferred Resistance to First-and Third-generation EGFR Tkis: a Case Report.
Authors: Zhang Meng , Huang Yihua , Chen Baorong , Peng Yuna , Lin Jing , Li Yuan , Chen Shuyin , Guan Liya , Yao Guohua , Fang Wenfeng 
Published: June 2020

Abstract
Background: The development of EGFR-tyrosine kinase inhibitors (EGFR-TKIs) have revolutionized the treatment paradigms for non-small cell lung cancer (NSCLC) patients. However, acquired resistance inevitably develops with a median progression-free survival (PFS) less than a year. The advancements in sequencing technologies have significantly promoted the identification of potential resistant mechanisms to targeted therapies.Case presentation: A 39-year-old Chinese male was diagnosed stage IVB NSCLC with EGFR exon 19 deletion (EGFR 19del) and received icotinib but got primary EGFR-TKI resistance caused by ROS1 fusion one month later. Then he received icotinib plus crizotinib and achieved partial response with a profession free survival of 4.8 months. Then he switched to osimertinib plus crizotinib due to the emergence of EGFR T790M mutation. TRIM24-BRAF fusion was detected after osimertinib failure, which might be potential resistant mechanism to osimertinib.Conclusions: This is the first case to confirm crizotinib plus icotinib in patients harboring both EGFR 19del and ROS1 fusion. This case displays the temporal heterogeneity of different resistant mechanisms emerging during disease course, highlighting the importance of dynamic genetic monitoring for better clinical managements.
Soil bacterial communities associated with stony soils influence the tuber size of Tetrastigma hemsleyanum
Authors: Guo Haipeng , Hong Chuntao , Zhang Jianhong , Qin Wensheng , Wei Bin 
Published: June 2020

Abstract
Background

Plants grown in stony soils have better-developed root systems and higher crop yields than those grown in non-stony soils. The roles of various physical and chemical effects of stony soils on plant growth have been published, but the roles of soil microbiota and rhizosphere microbiota have not been investigated.
Methods

Tetrastigma hemsleyanum plants were cultivated for two years in stony soils and in the same soil from which rock-fragments had been removed. The microbiome and the tuber transcriptome were analyzed, using multiple bioinformatics methods.
Results

The soil microbiota of these two soils were markedly different, and the stony soils contained high abundances of bacterial taxa belonging to the Actinobacteria, Rokubacteria, Rhizobiales, Desulfarculaceae, and Chthoniobacteraceae. These discriminatory taxa in soils may promote the tuber growth of T. hemsleyanum, through releasing nutrients from rocks and colonizing the rhizosphere and tuber surface of T. hemsleyanum. In addition, stony soils induced a dramatic change in the tuber’s transcriptome, particularly with respect to the pathways of phytohormone biosynthesis, photosynthesis, and biotic stress resistance, expression levels of which showed strong correlations with the aforementioned bacterial taxa.
Conclusions

These results indicated that beneficial effects of stony soils on plant growth may be closely correlated with their specific microbiota, which can, in turn, influence multiple biological processes of host. This is the first study to reveal the role of stony soils-driven microbiota in tuber growth, and stony soils can represent a microbial repository for the screening of microbial isolates to increase plant yield.
Low Dose Hyperbaric Bupivacaine and Dexmedetomidine as an Adjuvant in Spinal Anaesthesia, Caesarean Section: A Randomized Controlled Trial
Authors: Nasr Ahmed Ashraf , Helal Safaa Mohamed , Soltan Wesam Eldin Abdelrahman 
Published: June 2020

Abstract
Background

Alpha-2 agonist, e.g. dexmedetomidine, is a non-opioid adjuvant with a substantial role in extending the analgesic duration of the subarachnoid block. Here, we aim to test the efficacy of adding dexmedetomidine to hyperbaric bupivacaine in the caesarean delivery, targeting enhanced postoperative analgesia and more stable hemodynamics.
Methods

40 parturient women scheduled for elective caesarean section were enrolled and randomly allocated to two groups. Low Dose – Dexmedetomidine (LD- DEX) group (n = 20) received 7 mg hyperbaric bupivacaine plus 10 µg dexmedetomidine, control group (n = 20) received 12 mg hyperbaric bupivacaine. Continuous normally distributed data were expressed as mean and standard deviation. Ordinal data and continuous data not fitting to the normal distribution curve were presented as medians (range) meanwhile categorical data were reported as percentage of the total number. Regarding the autonomic variables, we did a stratified analysis to compare the mean change of these variables every 3 minutes till 60 minutes through a two-sample t-test (Welch t-test). All statistical analyses were performed using R software version 3.4.4
Results

LD- DEX groups showed significant faster and prolonged sensory block (P-value < 0.05), stable maternal hemodynamic maintained by less amount of IV fluids (P-value < 0.01), lower doses of ephedrine (P-value = 0.02), no signs of foetal distress, and low incidence of postoperative maternal shivering. As well, 3 hours cut off pain was more significant in LD- DEX group (P-value < 0.01).
Conclusion

LD- DEX group provided an optimum intraoperative condition and postoperative analgesia with less maternal side effects and no neonatal risks, encouraging enhanced recovery and less hospital in-stay.
Based on Exosome-Derived Genes for Constructing Diagnostic, Prognostic and Recurrence Models and Predicting Therapeutic Response for Hepatocellular Carcinoma
Authors: Ji Jiansong , Tang Bufu , Zhu Jinyu , Gao Yang , Tu Jianfei , Yang Weibin , Fang Shiji , Weng Qiaoyou , Zhao Zhongwei , Xu Min , Yang Yang , Chen Minjiang , Lu Chenying 
Published: June 2020

Abstract
Background: In this study, we performed a comprehensive analysis to identify the optimal gene set derived from exosomes in hepatocellular carcinoma (HCC) with great prognostic value to construct prognostic, recurrence and diagnostic models and predicted therapeutic response for hepatocellular carcinoma.Methods: Differential expression analysis and Weighted Gene Co-Expression Network Analysis (WGCNA) of RNA-sequencing were used to identify differentially expressed exosome-derived genes related to HCC. Univariate and multivariate Cox regression analyses were performed to determine exosome-derived genes associated with prognosis of HCC to construct a prognostic model, and recurrence and diagnostic models were built based on the same genes. Results: MYL6B and THOC2 were determined to be closely related to prognosis in HCC. The prognostic and recurrence models built based on these two genes were confirmed to be independent predictive factors with superior predictive performance. Patients with high prognostic risk had poorer prognosis than patients with low prognostic risk in all HCC sets, namely, the TCGA cohort (HR=2.5, P<0.001), ICGC cohort (HR=3.15, P<0.001) and GSE14520 cohort (HR=1.85, P=0.004). A higher recurrence probability was found in patients with high recurrence risk than in patients with low recurrence risk in the HCC sets of the TCGA cohort (HR=2.44, P<0.001) and GSE14520 cohort (HR=1.54, P=0.025). High prognostic risk patients had higher expression of immune checkpoints, such as PD1, B7H3, B7H5, CTLA4 and TIM3 (P<0.05). Patients with higher prognostic risk showed higher resistance to some chemotherapy and targeted drugs, such as methotrexate, erlotinib and vinorelbine (P<0.05). The diagnostic models based on the same two genes were determined to accurately distinguish HCC from normal subjects and dysplastic nodules.Conclusions: Prognostic, recurrence, and diagnostic models based on the two exosome-derived genes (MYL6B and THOC2) were proven superior predictive performance. Our findings lay the foundation for identifying molecular markers to increase the early detection rate of HCC, improve disease outcomes, and determine more effective individualized treatment options for patients.
Patients With Chronic Migraine Without History of Medication Overuse Are Characterized by a Peculiar White Matter Fiber Bundle Profile
Authors: Coppola Gianluca , Renzo Antonio Di , Tinelli Emanuele , Petolicchio Barbara , Lorenzo Cherubino Di , Parisi Vincenzo , Serrao Mariano , Calistri Valentina , Tardioli Stefano , Cartocci Gaia , Caramia Francesca , Piero Vittorio Di , Pierelli Francesco 
Published: June 2020

Abstract
Background: We investigated intracerebral fiber bundles using a tract-based spatial statistics (TBSS) analysis of diffusion tensor imaging (DTI) datato investigate microstructural integrity in patients with episodic (MO) and chronic migraine (CM).Methods: We performed DTI in 19 patients with MO within interictal periods, 18 patients with CM without any history of drug abuse, and 18 healthy controls (HCs) using a 3T magnetic resonance imaging scanner. We calculated diffusion metrics, including fractional anisotropy (FA), axial diffusion (AD), radial diffusion (RD), and mean diffusion (MD).Results: TBSS revealed no significant differences in the FA, MD, RD, and AD maps between the MO and HC groups. In comparison to the HC group, theCM group exhibited widespread increased RD (bilateral superior [SCR] and posterior corona radiata [PCR], bilateral genu of the corpus callosum [CC], bilateral posterior limb of internal capsule [IC], bilateral superior longitudinal fasciculus [LF]) and MD values (tracts of the right SCR and PCR, right superior LF, and right splenium of the CC). In comparison to theMO group, theCM group showed decreased FA (bilateral SCR and PCR, bilateral body of CC, right superior LF, right forceps minor) and increased MD values (bilateral SCR and right PCR, right body of CC, right superior LF, right splenium of CC, and right posterior limb of IC). Conclusion: Our results suggest that chronic migraine can be associated withthe widespread disruption of normal white matter integrity in the brain.
Inflammation and Endothelial Injury Profiling in COVID-19 Pediatric Multi-system Inflammatory Syndrome
Authors: Fraser Douglas , Patterson Eric , Daley Mark , Cepinskas Gedaminas 
Published: June 2020

Abstract
Background

COVID-19 is associated with a novel multi-system inflammatory syndrome that shares some characteristics with Kawasaki’s Disease. The syndrome manifestation is delayed relative to COVID-19 onset, with a spectrum of clinical severity. Clinical signs may include persistent fever, gastrointestinal symptoms, cardiac inflammation and/or shock.
Case Presentation:

We measured 59 inflammatory and endothelial injury plasma analytes in an adolescent girl that presented with malaise, fever, cough, strawberry tongue and jaundice. Her COVID-19 status was positive with detection of 2 SARS-CoV-2 viral genes using polymerase chain reaction. She was treated with intravenous immunoglobulin prior to blood draw, but our plasma measurements suggested a unique analyte expression pattern associated with inflammation, endothelial injury and microvascular glycocalyx degradation.
Conclusions

COVID-19 is associated with a multi-system inflammatory syndrome and a unique inflammatory and endothelial injury signature. Analyte markers of inflammation and endothelium injury might serve as putative biomarkers and/or be investigated further as potential therapeutic targets.
Effect of the extent of short-duration exercise stress combined with adenosine triphosphate on the artifacts of two different regions on myocardial perfusion single-photon emission computed tomography
Authors: Shinoda Yukinori , Tachibana Koichi , Minamisaka Tomoko , Fukuoka Hidetada , Inui Hirooki , Ueno Keisuke , Inoue Soki , Mine Kentaro , Ueda Kumpei , Hoshida Shiro 
Published: June 2020

Abstract
Background: We aimed to evaluate the differences in the reduction of undesired artifacts among different low-grade exercise stress levels combined with drug stress and their related factors by using myocardial perfusion single-photon emission computed tomography (SPECT) in patients with suspicious coronary artery disease.Methods: We divided patients into 4 groups as follows: group A, adenosine triphosphate (ATP) for 6 min; group A+25 W, ATP+25 W exercise for 6 min; group A+35 W, ATP+35 W exercise for 6 min; group A+45 W, ATP+45 W exercise for 6 min) and enrolled only those whose summed stress scores were <3. Undesired artifacts were evaluated on the basis of heart-to-liver activity (H/L) ratio and heart-to-10 pixels below the heart (H/below the H) ratio. Results: The logarithmic values of H/L and H/below the H ratios were significantly higher in groups A+35 W and A+45 W than in group A. In all the patients, the logarithmic values of H/L and H/below the H ratios positively correlated with heart rate (p=0.004 and p=0.008, respectively), rate pressure product (RPP; p=0.005 and p=0.008, respectively), and the increment of RPP (p=0.002 and p=0.005, respectively) after stress in the univariate analysis. The left ventricular end-diastolic volume (LVEDV) after stress (p=0.002) negatively correlated with the logarithmic value of H/below the H ratio, but not H/L ratio. Although male sex was independently associated with the logarithmic values of both H/L (p=0.014) and H/below the H ratios (p=0.001) in multivariate regression analysis, LVEDV was also independently associated with the logarithmic value of H/below the H ratio (p<0.001).Conclusion: ATP plus ≥35 W exercise stress for 6 min was useful for reducing undesired artifacts after stress in myocardial perfusion SPECT. LVEDV after stress was independently associated with the H/below the H ratio, but not the H/L ratio.
Evaluating the Implementation of the SWITCH® School Wellness Intervention Through Mixed Methods
Authors: McLoughlin Gabriella M , Candal Priscila , Vazou Spyridoula , Lee Joey A , Dzewaltowski David A , Rosenkranz Ric R , Lanningham-Foster Lorraine , Gentile Douglas A , Liechty Laura , Chen Senlin , Welk Gregory J 
Published: June 2020

Abstract
Background: School wellness programming is important for promoting academic achievement and healthy lifestyles in youth; however, research is needed on methods that can help schools implement and sustain programs on their own. The purpose of this study was to investigate factors within and outside the school environment that impacted school capacity for implementation and potential sustainability of wellness programming. Methods: As part of the School Wellness Integration Targeting Child Health (SWITCH®) intervention, elementary school wellness teams (N=30) were guided through a capacity-building process focused on promoting the adoption of healthy lifestyle behaviors in students. Data on implementation were collected through three standardized surveys and interviews (pre-mid-post) and a post-implementation interview. Indicators of organizational capacity were assessed using the School Wellness Readiness Assessment (SWRA). Paired t-tests were run to assess changes in implementation (classroom, physical education, lunchroom settings), capacity, and stakeholder engagement over time. One-way analysis of variance (ANOVA) tests were run to examine how implementation of best practices (low, moderate, high) explained differences in capacity gains. Qualitative data were analyzed through inductive and deductive analysis, following the Consolidated Framework for Implementation Research (CFIR). Results: Paired t-tests showed non-significant increases in school and setting-specific capacity and implementation of SWITCH best practices over time, in addition to a consistent level of engagement from key stakeholders. ANOVA analyses revealed positive, non-significant variances between implementation group and gains in school capacity (F[2,24]=1.63; p=.21), class capacity (F[2,24]=0.20 p=.82), lunchroom capacity (F[2,24]=0.29; p=.78), and physical education (F[2,24]=1.45; p=.25). Qualitative data demonstrated factors within the outer setting (i.e., engaging community partners) that facilitated programming. Inner-setting factors (i.e., relationships with administration and staff) significantly affected implementation. Implementation process construct themes (e.g., planning, adaptation of resources to meet school capacity/needs, and engaging students as leaders) were cited as key facilitators. Schools discussed factors impacting sustainability, such school culture and knowledge of school wellness policy. Conclusions: Findings suggest a modest but important impact of implementation on capacity change which is the primary goal of SWITCH. The results document the importance of allowing schools to adapt programming to meet their local needs.Trial Registration: NCT04045288