bluuebunny/crossref_metadata_2025_split huggingface dataset:
An antibody panel for highly specific detection and differentiation of Zika virus
Authors: Kabir Md Alamgir , Soto-Acosta Ruben , Sharma Sandhya , Bradrick Shelton S. , Garcia-Blanco Mariano A. , Caputi Massimo , Asghar Waseem 
Published: July 2020

AbstractZika virus (ZIKV) is an emerging flavivirus transmitted to humans by Aedes mosquitos. ZIKV can be transmitted from mother to fetus during pregnancy and can cause microcephaly and other birth defects. Effective vaccines for Zika are yet to be approved. Detection of the ZIKV is based on serological testing that often shows cross-reactivity with the Dengue virus (DENV) and other flaviviruses. We aimed to assemble a highly specific anti-Zika antibody panel to be utilized in the development of a highly specific and cost-effective ZIKV rapid quantification assay for viral load monitoring at point-of-care settings. To this end, we tested the affinity and specificity of twenty one commercially available monoclonal and polyclonal antibodies against ZIKV and DENV envelope proteins utilizing nine ZIKV and twelve DENV strains. We finalized and tested a panel of five antibodies for the specific detection and differentiation of ZIKV and DENV infected samples.
The role of chronotype in the interaction between the alerting and the executive control networks
Authors: Martínez-Pérez Víctor , Palmero Lucía B. , Campoy Guillermo , Fuentes Luis J. 
Published: July 2020

AbstractChronotype refers to the time of day preferred by individuals to perform daily activities according to their circadian rhythm. We asked whether synchrony effects, that is, the difference in performance between the optimal and non-optimal time of day as a function of chronotype, are observed in two tasks that differently involve the endogenous component of the alerting network, the psychomotor visual task (PVT) and the flanker task. From an initial sample of 132 students that filled in the Morningness–Eveningness Questionnaire (MEQ), 18 were classified as Morning-types and 16 as Evening-types. Evening-types showed synchrony effects in both tasks, whereas Morning-types failed to show synchrony effects in the flanker task and when the PVT was first performed at the non-optimal time of day. Thus, Morning-types might have seen increased their vigilant attention at their non-optimal time of day due to the cognitive demands of the flanker task and to the novelty with the PVT. Phasic alerting generated by alerting tones increased conflict score in the flanker task, but time of day did not modulate the congruence effect. Chronotype determines vigilant attention more decisively in Evening-types than in Morning-types individuals. Also, exogenous but not endogenous alerting exerts a deleterious effect on conflict resolution.
Conjuncted photo-thermoelectric effect in ZnO–graphene nanocomposite foam for self-powered simultaneous temperature and light sensing
Authors: Zhao Huiqi , Ouyang Bangsen , Han Lu , Mishra Yogendra Kumar , Zhang Zhiqiang , Yang Ya 
Published: July 2020

AbstractThe self-powered sensors are more and more important in current society. However, detecting both light and temperature signals simultaneously without energy waste and signal interference is still a challenge. Here, we report a ZnO/graphene nanocomposite foam-based self-powered sensor, which can realize the simultaneous detection of light and temperature by using the conjuncted photo-thermoelectric effect in ZnO–graphene nanocomposite foam sensor. The output current under light, heating and cooling of the device with the best ZnO/graphene ratio (8:1) for the foam can reach 1.75 µA, 1.02 µA and 0.70 µA, respectively, which are approximately three fold higher than them of devices with other ZnO/graphene ratios. The ZnO–graphene nanocomposite foam device also possesses excellent thermoelectric and photoelectric performances for conjuncted lighting and heating detection without mutual interference. The ZnO–graphene nanocomposite foam device exhibits a new designation on the road towards the fabrication of low cost and one-circuit-based multifunction sensors and systems.
Molecular characterization and gene expression modulation of the alternative oxidase in a scuticociliate parasite by hypoxia and mitochondrial respiration inhibitors
Authors: Folgueira Iría , Lamas Jesús , Sueiro Rosa Ana , Leiro José Manuel 
Published: July 2020

AbstractPhilasterides dicentrarchi is a marine benthic microaerophilic scuticociliate and an opportunistic endoparasite that can infect and cause high mortalities in cultured turbot (Scophthalmus maximus). In addition to a cytochrome pathway (CP), the ciliate can use a cyanide-insensitive respiratory pathway, which indicates the existence of an alternative oxidase (AOX) in the mitochondrion. Although AOX activity has been described in P. dicentrarchi, based on functional assay results, genetic evidence of the presence of AOX in the ciliate has not previously been reported. In this study, we conducted genomic and transcriptomic analysis of the ciliate and identified the AOX gene and its corresponding mRNA. The AOX gene (size 1,106 bp) contains four exons and three introns that generate an open reading frame of 915 bp and a protein with a predicted molecular weight of 35.6 kDa. The amino acid (aa) sequence of the AOX includes an import signal peptide targeting the mitochondria and the protein is associated with the inner membrane of the mitochondria. Bioinformatic analysis predicted that the peptide is a homodimeric glycoprotein, although monomeric forms may also appear under native conditions, with EXXH motifs associated with the diiron active centers. The aa sequences of the AOX of different P. dicentrarchi isolates are highly conserved and phylogenetically closely related to AOXs of other ciliate species, especially scuticociliates. AOX expression increased significantly during infection in the host and after the addition of CP inhibitors. This confirms the important physiological roles of AOX in respiration under conditions of low levels of O2 and in protecting against oxidative stress generated during infection in the host.
Fe-functionalized paramagnetic sporopollenin from pollen grains: one-pot synthesis using ionic liquids
Authors: Chiappe C. , Rodriguez-Douton M. J. , Mozzati M. C. , Prete D. , Griesi A. , Guazzelli L. , Gemmi M. , Caporali S. , Calisi N. , Pomelli C. S. , Rossella F. 
Published: July 2020

AbstractThe preparation of Fe-decorated sporopollenins was achieved using pollen grains and an ionic liquid as solvent and functionalizing agent. The integrity of the organic capsules was ascertained through scanning electron microscopy studies. The presence of Fe in the capsule was investigated using FT-IR, X-ray photoemission spectroscopy and energy-dispersive X-ray spectroscopy. Electron paramagnetic resonance and magnetization measurements allowed us to demonstrate the paramagnetic behavior of our Fe-functionalized sporopollenin. A few potential applications of pollen-based systems functionalized with magnetic metal ions via ionic liquids are discussed.
Long-term bowel dysfunction following low anterior resection
Authors: Dulskas Audrius , Kavaliauskas Povilas , Pilipavicius Lukas , Jodinskas Mantas , Mikalonis Martynas , Samalavicius Narimantas E. 
Published: July 2020

AbstractStudy aimed to assess long-term bowel function in patients who underwent low anterior resection for cancer five and more years ago. Patients who underwent low anterior resection for rectal cancer from 2010 to 2015 at National Cancer Institute were prospectively included in our study. They were interviewed using low anterior resection syndrome (LARS) score and Wexner questionnaire. We also assessed possible risk factors of postoperative bowel disorder. 150 patients were included in our study. Of them 125 (83.3%) were analysed. The median age at diagnosis was 62 years (40–79), and the average time of follow-up was 7.5 years (5–11). Overall, 58 (46.4%) patients had LARS, of them 33 (26.4%)—major LARS and 25 (20%)—minor LARS and 67 (53.6%) reported no LARS. Wexner score results were: normal in 43 (34.4%) patients, minor faecal incontinence—55 (44%), average faecal incontinence—18 (14.4%), complete faecal incontinence—9 (7.2%). 51 patients (40.8%) had tumour in the upper third rectum, 51 (40.8%)—in the middle and 23 (18.4%)—lower third. Preoperative (chemo)radiotherapy was the only significant risk factors for developing LARS in univariate analysis. Our study showed that only preoperative radiotherapy may be associated with more late problems in defecation after rectal cancer surgery.Trial registration: NCT03920202.
MicroRNAs in ascending thoracic aortic aneurysms
Authors: Moushi Areti , Pillar Nir , Keravnou Anna , Soteriou Marinos , Shomron Noam , Cariolou Marios A. , Bashiardes Evy 
Published: July 2020

AbstractThoracic Aortic Aneurysm (TAA) is characterized by the dilation of the aorta and is fatal if not diagnosed and treated appropriately. The underlying genetic mechanisms have not been completely delineated, so better knowledge of the physiopathology of TAAs is needed to improve detection and therapy. MicroRNAs (miRNAs) regulate gene expression post-transcriptionally and are known to be involved in cardiovascular diseases (CVDs). The current study aimed to identify miRNAs that can be used as possible biomarkers for the early diagnosis of patients with ascending TAAs (ATAAs). MiRNA expression was profiled by NanoString nCounter technology using 12 samples including tissue and pre- and post-surgical plasma from ATAA patients. Four miRNAs were selected and further validated by real time polymerase chain reaction (RT-PCR) in 22 plasma samples from which three miRNAs (hsa-miR140-5p, hsa-miR-191-5p and hsa-miR-214-3p) showed significant expression level differences between the two types of plasma samples. Further analyses of the corresponding predicted target genes by these miRNAs, revealed two genes (Myotubularin-related protein 4 (MTMR4) and Phosphatase 1 catalytic subunit β (PPP1CB)) whose expression was inversely correlated with the expression of their respective miRNAs. Overall, in this pilot study, we identified three miRNAs that might serve as potential biomarkers and therapeutic targets in ATAA.
Validity of Using Velocity to Estimate Intensity in Resistance
Exercises in Men and Women
Authors: Pareja-Blanco Fernando , Walker Simon , Häkkinen Keijo 
Published: July 2020

AbstractThis study aimed to examine the validity of using bar velocity to estimate
relative load in squat and bench-press exercises for both young men and
women. Twenty-five men and 25 women performed a progressive loading test up
to 1-RM in the squat and bench-press exercises, which were repeated after
2-weeks. Relationships between mean propulsive velocity and%1-RM
were analysed. A second-order polynomial equation for predicting the
corresponding velocity of each percentage of 1-RM was developed for men
(validation). This equation was then applied in women (cross-validation).
Moreover, a specific equation for women was developed (validation) and was
also applied in a sub-sample of women (cross-validation). Close
relationships (R2: 0.91–0.95) between bar velocity and
relative load were observed in both sexes for squat and bench press.
Men’s equation applied to women showed a high level of agreement,
although lower bias and higher level of agreement was observed when a
sex-specific equation was applied in women, both validation and
cross-validation samples. In conclusion, lifting velocity can be used to
accurately prescribe the relative load regardless of sex in both upper-body
and lower-body exercises, although when estimating load from velocity
measures it will be necessary to use the sex-specific equation for each
exercise.
Single-bite versus double-bite technique for mapping biopsies during endoscopic surveillance for hereditary diffuse gastric cancer: a single-center, randomized trial
Authors: Pappas Apostolos , Tan Wei Keith , Waldock William , Richardson Susan , Tripathi Monika , Januszewicz Wladyslaw , Roberts Geoffrey , O'Donovan Maria , Fitzgerald Rebecca C. , di Pietro Massimiliano 
Published: July 2020

Abstract
Background Endoscopic surveillance is recommended in patients with hereditary diffuse gastric cancer (HDGC) who refuse or want to delay surgery. Because early signet-ring cell carcinoma (SRCC) can be inconspicuous, the current surveillance endoscopy protocol entails 30 random biopsies, which are time-consuming. This study aimed to compare single-bite and double-bite techniques in HDGC surveillance.
Methods Between October 2017 and December 2018, consecutive patients referred for HDGC surveillance were prospectively randomized to the single- or double-bite arm. The primary outcome was the diagnostic yield for SRCC foci. Secondary outcomes were: procedural time for random biopsies; comfort score; biopsy size; and quality of specimens, the latter assessed by the presence of muscularis mucosa, crush artifact, and proportion usable for diagnostic assessment.
Results 25 patients were randomized to the single-bite arm and 23 to the double-bite arm. SRCC foci were detected in three and four patients in the single- and double-bite arms, respectively (P = 0.70). The procedural time for the double-bite arm (12 minutes, interquartile range [IQR] 4) was significantly shorter than for the single-bite arm (15 minute, IQR 6; P = 0.01), but comfort scores were similar. The size of the biopsies in the double-bite arm was significantly smaller than in single-bite arm (2.5 mm vs. 3.0 mm; P < 0.001) but this did not affect the presence of muscularis mucosa (P = 0.73), artifact level (P = 0.11), and diagnostic utility (P = 0.051).
Conclusion For patients undergoing HDGC surveillance, the double-bite technique is significantly faster than the single-bite technique. The diagnostic yield for SRCC and the biopsy quality were similar across both groups.
LI-RADS in the year 2020 – Are you already using it or still considering?
Authors: Ringe Kristina Imeen , Gut Anne , Grenacher Lars , Juchems Markus , Kukuk Guido , Wessling Johannes , Schreyer Andreas G. 
Published: July 2020

Purpose The working group for gastrointestinal and abdominal imaging within the German Radiological Society performed a nationwide online survey in order to assess the current status regarding the awareness and application of LI-RADS, a classification for evaluation of liver lesions in patients at risk.
Materials and Methods Using the website www.deutsches-krankenhausverzeichnis.de a list of hospitals was generated meeting the criteria internal medicine, gastroenterology, general and visceral surgery and radiology (n = 391). Randomly, 102 department directors were contacted, and asked to name one consultant and one resident from their department in order to participate in the survey. 177 potential participants were invited to fill out an approximately 10-minute online survey in the form of 17 questions regarding the awareness and application of LI-RADS. The results of the survey were analyzed by means of descriptive statistics.
Results 77 participants were registered, which corresponds to a response rate of 43.5 %. 47 % of all participants were radiologists, 30 % surgeons and 23 % internal doctors/gastroenterologists, respectively, many with more than 13 years of professional experience (37.2 %). The majority of participants worked in a hospital with a focus (37.2 %) or a university hospital (29.1 %). Even though the majority of participants knows about or has heard of LI-RADS (73.2 %), only a minority uses the classification themselves (26 %) or within the context of tumor boards (19.2 %).
Conclusion The results of our survey demonstrate that LI-RADS is relatively known in Germany, the application however quite sparse. This is in contrast to the general desire and endeavor for more standardized reporting in radiology.
Key points

Citation Format
Oversized versus Non-oversized Prosthesis: Midterm Outcomes after Transcatheter Aortic Valve Replacement Using SAPIEN 3 Valve
Authors: Useini Dritan , Beluli Blerta , Christ Hildegard , Mügge Andreas , Patsalis Polykarpos , Schlömicher Markus , Haldenwang Peter , Bechtel Matthias , Strauch Justus 
Published: July 2020

Abstract
Background A certain degree of prosthesis oversizing is recommended for the SAPIEN 3 (S3; Edwards Lifesciences Corp., Irvine, California, United States) to ensure device success. We aimed to investigate midterm outcomes in patients who received oversized (OS) S3 valve after transapical–transcatheter aortic valve replacement (TA-TAVR).
Methods Out of 122 patients with aortic stenosis who underwent TA-TAVR using S3 at our institution, 42 received OS prosthesis. We used computed tomography (CT) derived effective diameter to assess oversizing. We defined oversizing if the labeled diameter of the selected valve for implantation was ≥2 mm bigger than the effective annulus diameter calculated by the annulus area. We conducted a midterm follow-up and compared the OS cohort with the non-OS (nOS) cohort.
Results The study groups showed similar risk score and age (STS [Society of Thoracic Surgery] score: 5.4 ± 3; mean age: 80.7 ± 5.7). The 30-day mortality was 7.1% in OS versus 2.4% in nOS. The 30-day all-stroke was 2.4% in OS versus 0% in nOS. The 1- and 3-year all-cause mortality were 28.5 and 42.8% in OS versus 21.9 and 26.8% in nOS, respectively. Midterm freedom from death and from cardiocerebral events was similar in both groups. Moderate/severe paravalvular leakage occurred in 0% in OS versus 5.4% in nOS. The postdilation rate was 7.1% in OS versus 15.3% in nOS. The rate of new permanent pacemaker implantation (PPI) was 15.7% in OS versus 9.3% in nOS. The STS score was detected as an independent predictor of mortality.
Conclusion Oversizing reduces the risk of device failure and intraprocedural postdilation but increases the risk of PPI. Early and midterm morbidity and mortality after OS and nOS with S3 are comparable.
Predicting the long-term stability of compact multiplanet systems
Authors: Tamayo Daniel , Cranmer Miles , Hadden Samuel , Rein Hanno , Battaglia Peter , Obertas Alysa , Armitage Philip J. , Ho Shirley , Spergel David N. , Gilbertson Christian , Hussain Naireen , Silburt Ari , Jontof-Hutter Daniel , Menou Kristen 
Published: July 2020

Significance
Observations of planets beyond our solar system (exoplanets) yield uncertain orbital parameters. Particularly in compact multiplanet systems, a significant fraction of observationally inferred orbital configurations can lead to planetary collisions on timescales that are short compared with the age of the system. Rejection of these unphysical solutions can thus sharpen our view of exoplanetary orbital architectures. Long-term stability determination is currently performed through direct orbital integrations. However, this approach is computationally prohibitive for application to the full exoplanet sample. By speeding up this process by up to five orders of magnitude, we enable precise exoplanet characterization of compact multiplanet systems and our ability to examine the stability properties of the multiplanet exoplanet sample as a whole.
Site-specific ubiquitination of pathogenic huntingtin attenuates its deleterious effects
Authors: Hakim-Eshed Vicky , Boulos Ayub , Cohen-Rosenzweig Chen , Yu-Taeger Libo , Ziv Tamar , Kwon Yong Tae , Riess Olaf , Phuc Nguyen Hoa Huu , Ziv Noam E. , Ciechanover Aaron 
Published: July 2020

Significance
The hallmark of neurodegenerative disorders (e.g., Alzheimer’s, Parkinson’s, and Huntington’s disease) is the generation of aggregated proteins in different brain regions. These large aggregates originate from smaller, invisible, and soluble oligomers/aggregates and are believed to be sequestered from essential cellular machineries and, therefore, to attenuate/postpone the pathologic process. That, in contrast to the smaller ones that are thought to inhibit basic cellular pathways by trapping their components. The role played in this process by ubiquitin—which normally targets proteins for degradation—is unknown. Here, we show that ubiquitination of pathogenic Htt is important for its efficient aggregation and probably sequestration. Abolishing its ubiquitination results in generation of numerous smaller and less visible aggregates that are particularly harmful to cells.
A numerical damped oscillator approach to constrained Schrödinger equations
Authors: Ögren M , Gulliksson M 
Published: July 2020

Abstract
This article explains and illustrates the use of a set of coupled dynamical equations, second order in a fictitious time, which converges to solutions of stationary Schrödinger equations with additional constraints. In fact, the method is general and can solve constrained minimization problems in many fields. We present the method for introductory applications in quantum mechanics including three qualitative different numerical examples: the radial Schrödinger equation for the hydrogen atom; the 2D harmonic oscillator with degenerate excited states; and a nonlinear Schrödinger equation for rotating states. The presented method is intuitive, with analogies in classical mechanics for damped oscillators, and easy to implement, either with coding or with software for dynamical systems. Hence, we find it suitable to introduce it in a continuation course in quantum mechanics or generally in applied mathematics courses which contain computational parts. The undergraduate student can, for example, use our derived results and the code (supplemental material (https://stacks.iop.org/EJP/41/065406/mmedia)) to study the Schrödinger equation in 1D for any potential. The graduate student and the general physicist can work from our three examples to derive their own results for other models including other global constraints.
Evaluation of laser diffraction-based particle size measurements using digital inline holography
Authors: Kumar S Santosh , He Zilong , Hogan Christopher J , Fredericks Steven A , Hong Jiarong 
Published: July 2020

Abstract
The measurements of size distribution of small particles (e.g. dusts, droplets, bubbles, etc) are critical for a broad range of applications in environmental science, public health, industrial manufacturing, etc. Laser diffraction (LD), a widely used method for such applications, depends on model-based inversion with underlying assumptions on particle properties. Furthermore, the presence of sampling biases such as velocity differentials are often overlooked in simple ex-situ calibrations, which introduces as an additional source of error. In contrast, digital inline holography (DIH), a single camera coherent imaging technique, can both measure particle size distributions without the need for a model-based inversion and can directly provide information on the shape characteristics of the particles. In this study, we evaluate the performance of an LD system in characterizing polydisperse droplets produced in a flat fan spray using in-situ DIH based imaging as a reference. The systematic differences in the two techniques are examined. A droplet-trajectory-based correction for the LD-inferred size distributions is proposed to compensate for the observed differences. We validate the correction using NIST standard polydisperse particles undergoing differential settling, and then apply the correction to polydisperse spray droplet measurements. The correction improves agreement between LD and DIH size distributions for droplets over two orders of magnitude, but with LD still underestimating the fraction of droplets at sizes above ∼1 mm. This underestimation is possibly linked to the complex oscillatory and rotational motion of droplets which cannot be faithfully captured by measurement or modelled by the correction algorithm without additional information.