bluuebunny/crossref_metadata_2025_split huggingface dataset:
Serum miR-27a is a biomarker for prognosis of non-small cell lung cancer patients receiving chemotherapy
Authors: Xie Erfu , Ji Yazhou , Lin Mingxin , Jin Yuexinzi , Zhang Shichang , Huang Lei , Sun Ruihong , Wang Fang , Pan Shiyang 
Published: February 2020

Abstract
Background: Previous studies suggest that miR-27a may be involved in cancer development and progression. The purpose of this article was to investigate the clinical value of miR-27a in evaluating the prognosis of chemotherapy in NSCLC patients.Methods: The apoptosis rates of the SPC-A1 cells treated with optical cisplatin were determined by flow cytometry in different time. Simultaneously, the expression of miR-27a in supernatants and cells were detected. Fifty-two newly diagnosed cases of NSCLC patients were recruited. All patients received the Gemcitabine and Cisplatin as the first-line chemotherapy and Docetaxel as the second-line chemotherapy. At the end of every chemotherapy cycle, a therapeutic evaluation was performed according to the RECIST criteria. The expression of serum miR-27a was detected in each cycle.Results: After treated with cisplatin of 2.5μg/mL, the apoptosis rates of the SPC-A1 cells were significantly greater than the paired untreated control groups in 12, 24, 48 and 72 h, the expression of miR-27a in supernatants and cells also showed consistent changes with the apoptosis and displayed a time-dependent change. The chi-square test showed the elevation of miR-27a after chemotherapy was more common in patients with PR compared with the patients who achieved NR (61.5% vs 30.8%, P=0.026). Kaplan-Meier survival analysis indicated that patients with decreased miR-27a have poor outcome compared with those with increased miR-27a patients (P=0.0051). Furthermore, dynamic changes with serum miR-27a with gradual increasing trend during chemotherapy predicted a good prognosis.Conclusions: Serum miR-27a may be an independent predictor for prognosis of NSCLC.
The reporting quality of the interventional animal experiments in Chinese journals-based on the evaluated results of the Animals in Research: Reporting In Vivo Experiments (ARRIVE) guidelines
Authors: Ma Bin , Zhao Bing , Jiang Yanbiao , Zhang Ting , Shang Zhizhong , Zhang Weiyi , Hu Kaiyan , Chen Fei , Mei Fan , Gao Qianqian , Zhao Li , Kwong Joey S.W. 
Published: February 2020

Abstract
Objective To evaluate retrospectively the reporting quality of animal experiments published in Chinese journals adhering to the Animals in Research: Reporting In Vivo Experiments (ARRIVE) guidelines. Methods We searched databases including CNKI, WanFang, VIP and CBM by July, 2018. Two well-trained reviewers screened and extracted articles independently. The ARRIVE guidelines were used to assess the quality of animal experiment reports. The compliance rate of every item was analyzed according to different published time.Results 4342 studies were included finally, of which 73.03% had been cited ≤ 5 times, only 29.04% (1261/4342) were published in journals of Chinese Science Citation Database. The assessment results showed that the compliance rate of more than half of sub-items (51.28%, 20/39) was less than 50% and 65.00% (13/20) of sub-items was less than 10%.Conclusion The reporting quality of animal experiments in Chinese journals is generally lower than moderate level. Along with the publication of the ARRIVE guidelines in 2010, the compliance rate of most items in the ARRIVE guidelines has been improved to some extent. However, some specific items of methods, results and discussion were not still reported sufficiently and integrally. Therefore, it is necessary to popularize the ARRIVE guidelines, advocate researchers to adhere to the ARRIVE guidelines in the future and especially to promote the use of the ARRIVE guidelines in specialized journals, so as to promote the design, implementation and reporting of animal experiments, and ultimately improve their quality.
Improving the usefulness of evidence concerning the effectiveness of implementation strategies for knowledge products in primary healthcare: Protocol for a series of systematic reviews
Authors: Zomahoun Hervé Tchala Vignon , Massougbodji José , Bussières André , Thomas Aliki , Kairy Dahlia , Uwizeye Claude Bernard , Rheault Nathalie , Charif Ali Ben , Diendéré Ella , Langlois Léa , Tchoubi Sébastien , Gaye Serigne Abib , Légaré France 
Published: February 2020

Abstract
Background : The literature on the implementation of knowledge products is extensive. However, this literature is still difficult to interpret for policymakers and other stakeholders when faced with choosing implementation strategies likely to bring about successful change in their health systems. This work has the particularity to examine the scope of this literature, and to clarify the effectiveness of implementation strategies for different knowledge products. Consequently, we aim to: 1) determine the strengths and weaknesses of existing literature overviews; 2) produce a detailed portrait of the literature on implementation strategies for various knowledge products; and 3) assess the effectiveness of implementation strategies for each knowledge product identified and classify them. Methods : We will use a three-phase approach consisting of a critical analysis of existing literature overviews, a systematic review of systematic reviews, and a series of systematic reviews and meta-analyses. We will follow the Cochrane Methodology for each of three phases. Our eligibility criteria are defined following a PICOS approach: Population , individuals or stakeholders participating in healthcare delivery, specifically, healthcare providers, caregivers, and end users; I ntervention, any type of strategy aiming to implement a knowledge product including, but not limited to, a decision support tool, a clinical practice guideline, a policy brief, or a decision-making tool, a one-pager, or a health intervention; Comparison, any comparator will be considered; Outcomes, Phases 1 and 2 – any outcome related to implementation strategies including, but not limited to, the measures of adherence/fidelity to the use of knowledge products, their acceptability, adoption, appropriateness, feasibility, adaptability, implementation costs, penetration/reach and sustainability; Phase 3 – any additional outcome related to patients (psychosocial, health behavioral, and clinical outcomes) or healthcare professionals (behavioral and performance outcomes); Setting , primary healthcare has to be covered. For each phase, two reviewers will independently perform the selection of studies, data extraction, and assess their methodological quality. We will analyze extracted data, and perform narrative syntheses and meta-analyses when possible. Discussion : Our results could inform not only the overviews’ methodology, but also the development of an online platform for the implementation strategies of knowledge products. This platform could be useful for stakeholders in implementation science.
The CXCL12/CXCR4 signaling is required for DPP4 regulation of spontaneous inhibitory postsynaptic currents (sIPSCs)
Authors: Zhang Yusong , Chen Zhiguo , Yang Zhishan , Han Yadi 
Published: February 2020

Abstract
Background: Previous data suggested that dipeptidyl peptidase-IV (DPP4) involved in the occurrence of febrile seizure (FS), but its potential mechanism remains to be determined. Here, we investigated whether DPP4 regulated gamma-aminobutyric acid (GABA) mediated spontaneous inhibitory postsynaptic currents (sIPSCs) via the downstream C-X-C Motif Chemokine Ligand 12 (CXCL12)/ C-X-C chemokine receptor type 4 (CXCR4) signaling in cultured hippocampal neurons submitted to hyperthermia(39.5-40°C). Methods: Whole cell patch- clamp method was used to test sIPSC in vitro after DPP4 inhibition or CXCL12 administration. The level of CXCL12 and CXCR4 was tested using western blot analysis. The effect of CXCR4 antagonist AMD3100 (5 mg/ml, i.c.v) on seizures were tested using electroencephalogram (EEG) in a FS model. Results: We found that pharmacological DPP4 inhibitor sitagliptin (Sita,100μM) treatment or siRNA-mediated DPP4 knockdown enhanced the mean amplitude and frequency of sIPSCs in vitro. DPP4 knockdown with siRNA increased protein level of CXCL12 and CXCR4. Furthermore, CXCL12 (10 nM) treatment enhanced inhibitory transmission by increasing the mean frequency and amplitude of sIPSCs in vitro. AMD3100 administration decreased seizure severity by increasing hippocampal GABA content in vivo. Conclusions: Our data suggest that CXCL12/CXCR4 signaling is required for DPP4 regulation of sIPSCs, supporting that DPP4 played a key role in the pathogenesis of FS.
Knowledge, attitude and practice of Sri Lankan women towards breast cancer: A cross sectional study
Authors: Ranaweera N.D. , Dinesha P.M.C. , Pathirage C.A.K. , Weerasinghe P.W.D.N. , Senarathna D.M.K.N. , Ariyasena G.A.G.M.S. , Galgamuwa G.L.S. , Wijesiri H.S.M.S.K. , Balawardhana K.A.S.J. 
Published: February 2020

Abstract
Introduction Breast cancer is a type of cancer that develops from breast tissue. Although the knowledge on breast cancer among women in Sri Lanka is high, their practice of breast self-examination is poor. This study was aimed to determine attitudes, practices and the awareness of early detection techniques and risk factors among women in Sri Lanka. Method A study was conducted between two groups in National Cancer hospital, Sri Lanka and a peripheral women clinic consisting 317 participants in each group. Self-administered questionnaire was used to collect data regarding the awareness of early detection techniques, signs and symptoms and risk factors for breast cancers. Results A total of 33.8% of peripheral women clinic and 65.0% in National Cancer hospital patients had good knowledge regarding risk factors. The knowledge of risk factors, signs and symptoms and screening methods about breast cancers was significantly high in patients attending to National cancer hospital. Older aged people have less awareness about screening methods compared to younger people. People with high education background and those have a family history of breast cancer had more aware about screening and therapeutic methods. Conclusion Comparative to the peripheral women clinic, patients attending to National Cancer Hospital had a fairly good knowledge, practice and attitude regarding the early detection techniques, risk factors, signs and symptoms.
TMEM100 negatively regulated by microRNA-106b facilitates cellular apoptosis by repressing survivin expression in NSCLC
Authors: Ma Jun , Yan Tingting , Bai Yongrui , Ye Ming , Ma Xiumei , Wu Jihong , Zhang Lei 
Published: February 2020

Abstract
Background Non-small-cell lung cancer (NSCLC) is a common malignant tumor. Nevertheless, the 5-year survival rate of NSCLC patients remains poor. Thus, finding critical factors involved in regulating the progression of NSCLC is important for providing potential treatment targets. Methods CCK-8, colony formation, caspase-3 activity, luciferase reporter assay, and western blot assays were utilized in this study. Results We found that TMEM100 was significantly downregulated in NSCLC tissues compared with paired peritumoral tissues. Decreased TMEM100 expression was associated with poor clinical outcomes in NSCLC patients. Moreover, TMEM100 overexpression inhibited colony formation and facilitated apoptosis via repressing survivin expression in NSCLC cells, whereas TMEM100 knockdown had the opposite effect. In addition, miR-106b, a microRNA with controversial roles in different human cancers, was upregulated in NSCLC and directly downregulated TMEM100 expression. The roles of miR-106b in cell survival were mitigated by the restoration of TMEM100. Conclusions The above results indicate that TMEM100 induces cell apoptosis and inhibits cell survival by serving as a tumor suppressor and that miR-106b-degraded TMEM100 expression defines a potentially oncogenic pathway in NSCLC.
Klotho Deficiency Aggravated Diabetes-induced Podocyte injury
Authors: Chen Zhi , Zhou Qing , Liu Cong , Zeng Yiping , Yuan Shaolong 
Published: February 2020

Abstract
Background: Diabetic nephropathy (DN) is a progressive disease, the main pathogeny of which is podocyte injury inducing glomerular filtration barrier and proteinuria. The occurrence and development of DN could be partly attributed to the reactive oxygen species (ROS) generated by mitochondria. However, researches on how mitochondrial dysfunction (MtD) ultimately causes DNA damage is poor.Methods: We generated streptozotocin (STZ)-induced diabetic mice with wild-type(C57BL/6J) or Klotho deficiency mice (KL+/-) and treated podocytes with high glucose (HG) to investigated the function of Klotho on HG-induced podocyte injury in vivo and in vitro.Results: The absence of Klotho aggravated diabetic phenotypes indicated by podocyte injury accompanied by elevated urea albumin creatinine ratio (UACR), creatinine, urea nitrogen. Then, Klotho deficiency could significantly aggravate DNA damage by increasing 8-OHdG and reducing OGG1. Finally, Klotho deficiency may promote MtD to promote 8-OHdG-induced podocyte injury.Conclusions: Klotho deficiency may promote diabetes-induced podocytic MtD and aggravate 8-OHdG-induced DNA damage by affecting OOG1.
Antibiotic Resistance Profile of Bacteria Isolated from Wastewater Systems in Eastern Ethiopia
Authors: Ayele Awugchew Teshome , Alemayehu Tadesse , Ayele Yohanis , Serda Biresaw , Marami Dadi , Busa Nigusie 
Published: February 2020

Abstract
Background : Antimicrobial resistance is one of the major public health challenges in the 21st century. In response, World Health Organizations launched a global action plan on antimicrobial resistance since 2015, which is, along with other objectives, aimed to strengthen knowledge of the spread of Antimicrobial resistance through surveillance and research. As wastewater systems are important reservoirs of communal human and animal bacteria, antibiotic residue and resistance genes they are considered as surveillance hotspots. Objective : To assess the antibiotic resistance profile of proposed environmental resistance indicator bacteria in selected wastewater systems of Eastern Ethiopia from Feb. 2018 to Oct. 2019. Method : Three wastewater management systems in Eastern Ethiopia, such as the activated sludge system of Dire Dawa University, waste stabilization pond of Haramaya University and a septic tank of Hiwot Fana Specialized University Hospital were monitored by sampled quarterly over 18 months. A total of 66 samples was collected from 11 sampling locations and a total of 722 Escherichia coli (151), Aeromonas spp. (142), P. aeruginosa (143), E. faecalis (144), and E. faecium (142) were isolated using selective culture media and biochemical tests. Their antimicrobial susceptibility was tested using standard Kirby-Bauer disk diffusion method on the surface of the Mueller-Hinton agar and the result was interpreted according to EUCAST guidelines. Multiple Antibiotic Resistance Index (MARI) was calculated for each isolate and its change in the course of treatment was evaluated. Result : The highest percentage of resistance was seen for ampicillin among isolates of hospital wastewater effluent which is 36(94.7%), 33(91.7%) and 32(88.9%) for E. coli, E. faecalis, and E. faecium respectively. Lower rate of resistance was seen isolates from activated sludge system and gentamicin for isolates of activated sludge wastewater treatment system which is 10(16.4%), 8(13.3%), 11(18.9%), and 12(20.3%) for E. coli, E. faecalis, E. faecium , and P. aeruginosa respectively. MERI value was found to be variable across monitored sites and the course of the wastewater treatment process. Conclusion : The study has found a high level of environmental antibiotic resistance indicator bacteria thrive in the wastewater systems most of which were multi-resistant. Hospital wastewater exhibits higher resistance than the other two wastewater systems. The multi-drug resistance index has significantly increased in the course of the wastewater treatment process, indicating the possible proliferation of resistance in the wastewater treatment system.
Prevention and Healing of Calcium Signaling Mediated Neuronal Damage on successive Administration of Flavonoid Enriched Pterocarpus Marsupium Roxb in Peripheral Neuropathy Model
Authors: Shaju Neethi , Gautam Mrinmoy , Khayum Abdul , Venkatesh Gunasekaran 
Published: February 2020

Background:
Modern research on peripheral neuropathy circumstance utter that treatments
with Vincristine (VCR) disturb the microtubular cells in sensory and motor neurons due to calcium
over- load in sciatic nerve, unfortunately, VCR triggering the release of Tumor necrosis factor-α
(TNFα) in central neurons causes excitotoxicity as well. Although ethnomedical information specifies
that Pterocarpus marsupium Roxb (PM) is widely used for various nervous disorders, not yet justified
on VCR induced peripheral neuropathy and in relation to central mechanism.

Objective:
This study is aimed to explore the possible central and peripheral mechanism of flavonoid
enriched PM in VCR induced neuropathy model.

Methods:
Neuropathic pain was induced in female Wistar rats by VCR (75μg/ kg/day, i.p) for 10 days.
Nociceptive thresholds were assessed by subjecting them to behavioral and biochemical estimation, proinflammatory
cytokines along with morphological evaluation.

Results:
PM significantly increased the nociceptive threshold evident from various behavioral models in
comparison to VCR group. More importantly, PM significantly reversed the VCR induced calcium elevation,
glutamate and aspartate release in the brain.

Discussion:
It was also observed that the raised TNF-α, Interleukin-1β were controlled and interleukin-
10 was elevated in sciatic nerve after PM treatment. Evident from histology, PM markedly reversed the
VCR induced axonal degeneration, Schwann cell hyperplasia, and myelin fibrosis.

Conclusion:
Flavonoid enriched PM both 100 & 200mg/kg post and co-administration exerted a preventive
and curative effect in VCR induced neuropathic pain by controlling calcium-mediated excitotoxicity
through peripheral and central mechanism.
Nanocrystalline ZnO: A Competent and Reusable Catalyst for the Preparation of Pharmacology Relevant Heterocycles in the Aqueous Medium
Authors: Saha Moumita , Das Asish R. 
Published: February 2020

:Nanoparticle catalyzed synthesis is a green and convenient method to achieve most of the chemical transformations in water or other green solvents. Nanoparticle ensures an easy isolation process of catalyst as well as products from the reaction mixture avoiding the hectic work up procedure. Zinc oxide is a biocompatible, environmentally benign and economically viable nanocatalyst with effectivity comparable to the other metal nanocatalyst employed in several reaction strategies. This review mainly focuses on the recent applications of zinc oxide in the synthesis of biologically important heterocyclic molecules under sustainable reaction conditions.:Application of zinc oxide in organic synthesis: Considering the achievable advantages of this nanocatalyst, presently several research groups are paying attention in anchoring zincoxide or its modified structure in several types of organic conversions e.g. multicomponent reactions, ligand-free coupling reactions, cycloaddition reaction, etc. The advantages and limitations of this nanocatalyst are also demonstrated. The present study aims to highlight the recent multifaceted applications of ZnO towards the synthesis of diverse heterocyclic motifs. Being a promising biocompatible nanoparticle, this catalyst has an important contribution in the fields of synthetic chemistry and medicinal chemistry.
Comparison of Regulatory Requirements for Pharmaceuticals in Non- European Union Member States Kosovo, Ukraine and Serbia
Authors: Jani Shivani , Patel Nilesh , Chotaliya Urvi , Patel Ashok 
Published: February 2020

Background:
Literature review suggested that regulatory guidelines should be harmonized
for better processing of applications and for the upliftment of the regulatory field. Therefore
it was thought worthwhile to compare the guidelines for countries where there is requirement of
harmonization. Kosovo, Ukraine and Serbia were selected because of being European countries
and still they are not a part of EU.

Introduction:
Kosovo, Ukraine and Serbia are small countries of Europe but they are not members
of European Union. They have their own guidelines for the submission of MAA for marketing
of pharmaceuticals and medical devices. They are trying to obtain the EU membership and
therefore it was worthwhile to compare the guidelines of these countries.

Method:
The registration process of pharmaceuticals in Kosovo, Ukraine and Serbia was studied
throughly. Along with it, the guidelines for European Union were also studied. A comparison of
guidelines of all the three countries with the guidelines of European Union for pharmaceuticals
was carried out.

Result:
The comparison of guidelines showed that there are still some changes needed in the
guidelines of Kosovo, Ukraine and Serbia before they can merge with the guidelines of European
Union. Some of the points in the guidelines are very different from the guidelines of EU.

Conclusion:
So it was worthwile to study the regulatory requirements of pharmaceuticals in Non-
European Union Member States Kosovo, Ukraine and Serbia.
ANALISIS OPEN GOVERNMENT PADA DIREKTORAT JENDEREAL ENERGI BARU, TERBARUKAN DAN KONSERVASI ENERGI, KEMENTERIAN ENERGI DAN SUMBER DAYA MINERAL REPUBLIK INDONESIA
Authors: Respitawulan Anggraeni 
Published: February 2020

The change paradign in energy demand side and supply side management make goverment should think creatively to increase nergy national mix 23% at 2025 with new and renewable energy development programme. This research aims to describe the open goverment strategy condition in new and renewable energy development programme use kualitatif method through interview and observation. In general pelaksaan open government have on the directorate general ebtke has been implemented in terms of planning, implementation and supervision activities. This reseach find that new and renewable energy programme basically has a good platform, proven from a lot of using information and technology to support the programme. But unfortunately many platform are not used optimally to increase tranparency, participation and collaboration as a open goverment theory to the new and renenwable energy programme. In the transparency aspect informatio technology has used to broadcast information, in participation aspect, public has no access to policy formulation. While in the collaboration aspect show with many cooperation between goverment, private sector, public, NGO and other institution or country in various new and renewable energy programme.
PENANGGULANGAN ANAK JALANAN BERBASIS COMMUNITY DEVELOPMENT DI KOTA SERANG
Authors: Hasanah Budi , Putri Liza Diniarizky 
Published: February 2020

Children must receive good education, positive parenting, protection and other rights so that they can growth properly. But actually, it did not happen in Kota Serang. It was caused by the increasing number of street children. There were increasing number of street children in 2016, 31 children so until now the total was 181 children. This research was about the prevention of street children based on community development with conflict theme and intervention model. This research used biddle theory with three indicators: explanatory, organizational and discussional. This research used qualitative with descriptive method. The data from interview and deep discussion as well as observation used interactive model analysis. The results showed that there were some factors behind the number of street children in Kota Serang. Based on the FGD results, an alternative solution was need to reactivation the existing halfway house and form special team to take care the childreen who live there. Thus, the prevention of street childreen based on community development program could be run effectively.