bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sapap3 deletion causes dynamic synaptic density abnormalities: a longitudinal [11C]UCB-J PET study in a model of obsessive–compulsive disorder-like behaviour
Authors: Glorie Dorien , Verhaeghe Jeroen , Miranda Alan , De Lombaerde Stef , Stroobants Sigrid , Staelens Steven 
Published: November 2020

Abstract
Background
Currently, the evidence on synaptic abnormalities in neuropsychiatric disorders—including obsessive–compulsive disorder (OCD)—is emerging. The newly established positron emission tomography (PET) ligand ((R)-1-((3-((11)C-methyl-(11)C)pyridin-4-yl)methyl)-4-(3,4,5-trifluorophenyl)pyrrolidin-2-one) ([11C]UCB-J) provides the opportunity to visualize synaptic density changes in vivo, by targeting the synaptic vesicle protein 2A (SV2A). Here, we aim to evaluate such alterations in the brain of the SAP90/PSD-95-associated protein 3 (Sapap3) knockout (ko) mouse model, showing an abnormal corticostriatal neurotransmission resulting in OCD-like behaviour.

Methods
Longitudinal [11C]UCB-J µPET/CT scans were acquired in Sapap3 ko and wildtype (wt) control mice (n = 9/group) to study SV2A availability. Based on the Logan reference method, we calculated the volume of distribution (VT(IDIF)) for [11C]UCB-J. Both cross-sectional (wt vs. ko) and longitudinal (3 vs. 9 months) volume-of-interest-based statistical analysis and voxel-based statistical parametric mapping were performed. Both [11C]UCB-J ex vivo autoradiography and [3H]UCB-J in vitro autoradiography were used for the validation of the µPET data.

Results
At the age of 3 months, Sapap3 ko mice are already characterized by a significantly lower SV2A availability compared to wt littermates (i.a. cortex − 12.69%, p < 0.01; striatum − 14.12%, p < 0.001, thalamus − 13.11%, p < 0.001, and hippocampus − 12.99%, p < 0.001). Healthy ageing in control mice was associated with a diffuse and significant (p < 0.001) decline throughout the brain, whereas in Sapap3 ko mice this decline was more confined to the corticostriatal level. A strong linear relationship (p < 0.0001) was established between the outcome parameters of [11C]UCB-J µPET and [11C]UCB-J ex vivo autoradiography, while such relationship was absent for [3H]UCB-J in vitro autoradiography.

Conclusions
[11C]UCB-J PET is a potential marker for synaptic density deficits in the Sapap3 ko mouse model for OCD, parallel to disease progression. Our data suggest that [11C]UCB-J ex vivo autoradiography is a suitable proxy for [11C]UCB-J PET data in mice.
LHH1, a novel antimicrobial peptide with anti-cancer cell activity identified from Lactobacillus casei HZ1
Authors: He Jun-Fang , Jin Du-Xin , Luo Xue-Gang , Zhang Tong-Cun 
Published: November 2020

Abstract
Antimicrobial peptides have been attracting increasing attention for their multiple beneficial effects. In present study, a novel AMP with a molecular weight of 1875.5 Da, was identified from the genome of Lactobacillus casei HZ1. The peptide, which was named as LHH1 was comprised of 16 amino acid residues, and its α-helix content was 95.34% when dissolved in 30 mM SDS. LHH1 exhibited a broad range of antimicrobial activities against Gram-positive bacteria and fungus. It could effectively inhibit Staphylococcus aureus with a minimum inhibitory concentration of 3.5 μM and showed a low hemolytic activity. The scanning electron microscope, confocal laser scanning microscope and flow cytometry results showed that LHH1 exerted its antibacterial activity by damaging the cell membrane of Staphylococcus aureus. Meanwhile, LHH1 also exhibited anti-cancer cell activities against several cancer cells via breaking the cell membrane of MGC803, HCT116 and C666-1 cancer cells.
Prospecting the antimicrobial and antibiofilm potential of Chaetomium globosum an endophytic fungus from Moringa oleifera
Authors: Kaur Navdeep , Arora Daljit Singh 
Published: November 2020

Abstract
The current study prospects the antimicrobial potential of an endophytic fungus Chaetomium globosum which showed a wide spectrum antimicrobial activity against the tested pathogenic microorganisms. This is apparently the first report where Chaetomium globosum as an endophyte from Moringa oleifera showed antimicrobial potential and is optimized for physiochemical parameters to enhance the antimicrobial metabolites production. In the classical optimization yeast peptone dextrose medium, inoculum size of two discs, incubation period of 6 days, production temperature of 25 ºC and pH 7 was best supportive for optimal growth and antimicrobial activity whereas maltose and ammonium nitrate were the best carbon and nitrogen sources, respectively. The statistical optimization resulted in up to 1.33 fold increase in antimicrobial activity. Chloroform was found to be the best extractant. The chloroformic extract showed minimum inhibitory concentration ranging from 0.05 to 5 mg/ml and its microbicidal nature was established by viable cell count studies. The efficacy of the extract was also established in terms of post antibiotic effect which ranged from 2 to 20 h. The chloroformic extract exhibited the good antibiofilm potential and was also found to be biosafe. The clinical relevance of the study was justified as it showed good antimicrobial efficacy against some resistant clinical isolates, too.
Unleashing the potential of QoS-aware pricing within licensed shared access framework
Authors: Douros Vaggelis G. , Mähönen Petri 
Published: November 2020

AbstractWe present a techno-economic analysis of a cellular market that operates within the licensed shared access (LSA) framework, consisting of a mobile network operator (MNO) that leases spectrum to a number of programme making and special events (PMSE) users. The MNO offers two quality-of-service (QoS) classes (high and low), differentiating the price based on the QoS class. The key question that we address is whether and to which extent the MNO has incentive to adopt this form of QoS-aware pricing. After getting some promising insights from the analysis of two case studies, we propose a general methodology. The first step is to model the parameters that are controlled by each PMSE user: (i) the way to choose between the two QoS classes and (ii) the available budget per QoS class. The second step is to compute the maximum revenue of the MNO. Our analysis reveals that the MNO can always tune the prices so as to maximise its revenue for the scenario where all users belong to the high QoS class. This is a consistent result throughout our study that holds for any considered set of user-controlled parameters and of technical parameters. We conclude that the adoption of QoS-aware pricing in an LSA market generates a tussle between the MNO and the regulator. The MNO has incentive to support fewer users but with high QoS and charge them more, which is not aligned with the regulator’s goal for social welfare maximisation.
Effect of grassland degradation on soil quality and soil biotic community in a semi-arid temperate steppe
Authors: Han Xu , Li Yuhui , Du Xiaofang , Li Yingbin , Wang Zhengwen , Jiang Siwei , Li Qi 
Published: November 2020

Abstract
Grasslands provide a number of ecosystem services for human society. Degradation of grasslands results in the loss of biodiversity and leads to the deterioration of ecosystem functions. In order to accurately assess the influence of grassland degradation on belowground ecosystems, we conducted experiments on a temperate steppe with different levels of degradation and investigated the influence of degradation on soil quality and soil biotic communities. Our results showed that grassland degradation significantly decreased soil quality, with lower values of soil quality index (SQI) observed in the degraded grassland than the meadow steppe and the grassland from the forest-steppe ecotone. Changes in the SQI along the grassland degradation gradient were positively correlated with soil carbon stock and the aboveground biomass, and negatively correlated with the root shoot ratio. Nematode trophic diversity and the ratio of fungal to bacterial PLFA were lower in the degraded grassland than the grassland from the forest-steppe ecotone. The dissimilarities in soil microbial and nematode community composition increased with the changes in soil quality index. Our results indicate that soil quality index based on the minimum data sets could effectively assess the influence of grassland degradation on soil biodiversity and ecosystem function. In order to effectively restore degraded grasslands, the key contributors to the soil quality, such as soil carbon, should be taken on priority basis for revitalizing the soil biodiversity and ecosystem function.
Effect of land use, season, and soil depth on soil microbial biomass carbon of Eastern Himalayas
Authors: Lepcha Nima Tshering , Devi N. Bijayalaxmi 
Published: November 2020

Abstract

Background
Soil microbial biomass, an important nutrient pool for ecosystem nutrient cycling is affected by several factors including climate, edaphic, and land-use change. Himalayan soils are young and unstable and prone to erosion and degradation due to its topography, bioclimatic conditions and anthropogenic activities such as frequent land-use change. Through this study, we tried to assess how soil parameters and microbial biomass carbon (MBC) of Eastern Himalayan soils originated from gneissic rock change with land-use type, soil depth and season. Chloroform fumigation extraction method was employed to determine MBC from different land-use types.

Results
Soil physical and chemical properties varied significantly with season, land-use and soil depth (p < 0.001). The maximum values of soil properties were observed in the rainy season followed by summer and winter season in all the study sites. Annual mean microbial biomass carbon was highest in the forest (455.03 μg g− 1) followed by cardamom agroforestry (392.86 μg g− 1) and paddy cropland (317.47 μg g− 1). Microbial biomass carbon exhibited strong significant seasonal difference (p < 0.001) in all the land-use types with a peak value in the rainy season (forest-592.78 μg g− 1; agroforestry- 499.84 μg g− 1 and cropland- 365.21 μg g− 1) and lowest in the winter season (forest − 338.46 μg g− 1; agroforestry – 320.28 μg g− 1 and cropland − 265.70 μg g− 1). The value of microbial biomass carbon decreased significantly with soil depth (p < 0.001) but showed an insignificant increase in the second year which corresponds to a change in rainfall pattern. Besides, land-use type, season and soil depth, soil properties also strongly influenced microbial biomass carbon (p < 0.001). Microbial quotient was highest in the agroforestry system (2.16%) and least in the subtropical forest (1.91%).

Conclusions
Our results indicate that land-use, soil depth and season significantly influenced soil properties and microbial biomass carbon. The physical and chemical properties of soil and MBC exhibit strong seasonality while the type of land-use influenced the microbial activity and biomass of different soil layers in the study sites. Higher soil organic carbon content in cardamom agroforestry and forest in the present study indicates that restoration of the litter layer through retrogressive land-use change accelerates microbial C immobilization which further helps in the maintenance of soil fertility and soil organic carbon sequestration.
Are non-market benefits of soybean production significant? An extended economic analysis of smallholder soybean farming in Upper West region of northern Ghana
Authors: Asodina Francis Akabo , Adams Faizal , Nimoh Fred , Weyori Emmanuel A. , Wongnaa Camillus Abawiera , Bakang John Edudes-Andvi 
Published: November 2020

Abstract

Background
Traditional cost–benefit analysis of soybean production tends to largely focus on financial benefits to farmers, and less so on non-market co-benefits in sustaining smallholder farming systems. Relying solely on the standard financial analysis undermines the actual benefit of soybean production, which often results in ineffectual policy designs. An economic analysis that incorporates key non-pecuniary co-benefits of soybean production provide vital insight that contributes to improving productivity and overall economic well-being of farmers. Cross-sectional data were collected from 271 farmers to estimate the overall economic benefit of soybean that captures both market and non-market attributes in three major producing districts (Sissala-West, Wa-East, and Dafiama-Busie-Issa (DBI)) of Ghana.

Results
When non-market co-benefits were omitted, soybean production was not profitable (−Gh¢103.10/ha or −US$22.91) in DBI while Sissala-West and Wa-East had modest profit margins. However, the financial analysis changed dramatically when an average non-market value of Gh¢345.69 (US$76.82) was incorporated in the analysis. The soybean system was, therefore, financially viable for all the districts when the non-market attributes of the crop were considered.

Conclusions
The findings demonstrate the importance of the non-pecuniary benefits of soybean in smallholder farming systems for policy decision-making. For instance, farmers’ motivation for soybean production is closely linked to those ancillary benefits like the biological nitrogen fixed in the soil for cultivation of other crops. Similarly, crop administrators and policy makers’ support for conservation agriculture and green environment is tied to these non-market co-benefits.
Advances of target therapy on NOTCH1 signaling pathway in T-cell acute lymphoblastic leukemia
Authors: Zheng Ruyue , Li Menglin , Wang Shujuan , Liu Yanfang 
Published: November 2020

AbstractT-cell acute lymphoblastic leukemia (T-ALL) is one of the hematological malignancies. With the applications of chemotherapy regimens and allogeneic hematopoietic stem cell transplantation, the cure rate of T-ALL has been significantly improved. However, patients with relapsed and refractory T-ALL still lack effective treatment options. Gene mutations play an important role in T-ALL. The NOTCH1 gene mutation is the important one among these genetic mutations. Since the mutation of NOTCH1 gene is considered as a driving oncogene in T-ALL, targeting the NOTCH1 signaling patheway may be an effective option to overcome relapsed and refractory T-ALL. This review mainly summarizes the recent research advances of targeting on NOTCH1 signaling pathway in T-ALL.
Molecular insights into pathogenesis and targeted therapy of peripheral T cell lymphoma
Authors: Xie Caiqin , Li Xian , Zeng Hui , Qian Wenbin 
Published: November 2020

AbstractPeripheral T-cell lymphomas (PTCLs) are biologically and clinically heterogeneous diseases almost all of which are associated with poor outcomes. Recent advances in gene expression profiling that helps in diagnosis and prognostication of different subtypes and next-generation sequencing have given new insights into the pathogenesis and molecular pathway of PTCL. Here, we focus on a broader description of mutational insights into the common subtypes of PTCL including PTCL not other specified type, angioimmunoblastic T-cell lymphoma, anaplastic large cell lymphoma, and extra-nodal NK/T cell lymphoma, nasal type, and also present an overview of new targeted therapies currently in various stages of clinical trials.
The significant role of redox system in myeloid leukemia: from pathogenesis to therapeutic applications
Authors: Kaweme Natasha Mupeta , Zhou Shu , Changwe Geoffrey Joseph , Zhou Fuling 
Published: November 2020

Abstract

Background
Excessive generation of reactive oxygen species (ROS) in the presence of a defective antioxidant system can induce cellular damage and disrupt normal physiological functions. Several studies have revealed the unfavorable role of ROS in promoting the growth, proliferation, migration, and survival of leukemia cells. In this review study, we summarize the mechanisms of ROS production and its role in leukemogenesis, counteractive effects of antioxidants, and implicate the current ROS-dependent anticancer therapies in acute myeloid leukemia.

Body
The dysregulation of the redox system is known to play a significant role in the pathogenesis of leukemia. Leukemia cells generate high levels of ROS, which further increases the levels through extra pathways, including mitochondrial deoxyribonucleic mutation, leukemic oncogene activation, increased nicotinamide adenine phosphate hydrogen (NADPH), and cytochrome P450 activities. Aforementioned pathways once activated have shown to promote genomic instability, induce drug resistance to leukemia medical therapy, disease relapse and reduce survival period. The current standard of treatment with chemotherapy employs the pro-oxidant approach to induce apoptosis and promote tumor regression. However, this approach retains several deleterious effects on the subject resulting in degradation of the quality of life. Nevertheless, the addition of an antioxidant as an adjuvant drug to chemotherapy alleviates treatment-related toxicity, increases chemotherapeutic efficacy, and improves survival rates of a patient.

Conclusion
Acute myeloid leukemia remains a daunting challenge to clinicians. The desire to achieve the maximum benefit of chemotherapy but also improve patient outcomes is investigated. ROS generated through several pathways promotes leukemogenesis, drug resistance, and disease relapse. Chemotherapy, the mainstay of treatment, further upregulates ROS levels. Therefore, the addition of an antioxidant to leukemia medical therapy alleviates toxicity and improves patient outcomes.
Biomarkers for cancer-associated fibroblasts
Authors: Han Chencheng , Liu Tongyan , Yin Rong 
Published: November 2020

Abstract
Cancer-associated fibroblasts (CAFs) are the key component of tumor stromal. High heterogeneity of CAFs reflects in their origin, phenotype and function. Biological function which can be suggested by biomarkers of distinct CAF subgroups may be different, even opposite, just like water and fire. Identifying CAF subpopulations expressing different biomarkers and reconciling the relationship of the “water and fire” among distinct CAF subsets may be a breakthrough in tumor therapy. Herein, we briefly summarize the biomarkers commonly used or newly identified for distinct CAFs in terms of their features and potential clinical benefits.
A collaborative effort of China in combating COVID-19
Authors: Bangura Mohamed S. , Gonzalez Maria J. , Ali Nasra M. , Ren Ran , Qiao Youlin 
Published: November 2020

Abstract
A novel coronavirus (COVID-19) was firstly identified in Wuhan by the end of 2019. China has implemented a series of preventive measures to deter COVID-19 spread and its consequences since the beginning of the epidemic. In China, the expansion of COVID-19 has been slowed down significantly through the effort of all contributors, including governments, nongovernments, and civil society. All collaborators have been actively involved in combating the epidemic, using their respective strengths to play their roles. China has mitigated the number of cases due to the mobilization of the whole society and collaborators joining collective efforts and actions in solidarity to tackle and conquer the virus. To date, China has continued to implement actions to control any resurgence of new cases of COVID-19 and keep its population safe. The people’s united effort against the virus has enhanced a great insight into China, and it might serve as a model to the global community in fighting the COVID-19 pandemic in terms of coordination, solidarity, decisiveness, and leadership.
Predictors of perioperative morbidity and mortality in adult living donor liver transplantations: report of results of a tertiary hospital
Authors: Hizarci Burcu , Karaaslan Pelin , Ertugrul Gokhan , Yilmaz Mesut , Demiraran Yavuz , Oz Huseyin 
Published: November 2020

Abstract

Background
The aim of this study was to investigate the risk factors effective in perioperative morbidity and mortality in 161 living donor liver transplantations (LDLT).

Results
The most common indication for living donor transplantation was cryptogenic cirrhosis. The most common complication was biliary problems in 62.16% cases. Sepsis was the most common cause of in 52%. Patients in whom sepsis was observed, significantly prolonged stay under mechanical ventilation and prolonged ICU stay were detected. In patient group in whom mortality was observed, higher amounts of erythrocytes, fresh frozen plasma (FFP), and platelets were transfused, and patients remained longer under mechanical ventilation treatment, and in the ICU.

Conclusion
Perioperative morbidity and mortality was found to be significantly related with higher amounts of erythrocytes and FFP transfusions and longer operative and warm ischemia times. Sepsis was found to be the most common cause of mortality.
Comparative study between continuous adductor canal block and intravenous morphine for postoperative analgesia in total knee arthroplasty
Authors: Ali Hithem Mohammed Gamil Mohammed , Nour Eldin Bassel Mohammed Essam , Ahmed Heba Abd Elazim Labib , Gobran Ramy Mounir Wahba , Heiba Diaa Eldin Mahmoud 
Published: November 2020

Abstract
Background
Postoperative pain after total knee arthroplasty (TKA) is known to range from moderate (30% of patients) to severe (60% of patients). Inadequate management for postoperative pain may induce various immobility-related complications, muscle weakness, and chronic pain. Therefore, post-TKA analgesia is crucial, not only for patients’ satisfaction, but for improving surgical outcomes and reducing complications. The present study aims to compare the effect of ultrasound-guided adductor canal block ACB (saphenous nerve block) versus incremental dose of intravenous morphine after total knee arthroplasty surgery.

Results
The results of this study revealed no difference between group A and group B as regards postoperative quadriceps muscle strength; maximal knee flexion, total distance ambulated, and postoperative vital data (heart rate per minute and respiratory rate per minute). However, group A showed better postoperative pain control, lower doses of intravenous morphine consumption and lower incidence of nausea and vomiting.

Conclusion
Continuous adductor canal block (saphenous nerve block) is superior to intravenous morphine in decreasing postoperative pain and decreasing total morphine consumption and adverse effects as nausea and vomiting, but both are equivalent in preserving quadriceps muscle power.
Study of homogenization on media milling time in preparation of irbesartan nanosuspension and optimization using design of experiments (DoE)
Authors: Borkhataria Chetan , Patel Dhavalkumar , Bhagora Swati , Patel Nilesh , Patel Kalpesh , Manek Ravi 
Published: November 2020

Abstract

Background
The present investigation aimed at preparing nanosuspension of irbesartan to improve its dissolution. Dissolution enhancement of irbesartan can improve the oral bioavailability. Here, it was also studied how media milling time can be reduced by subjecting irbesartan to prior homogenization and then media milling.

Results
First, homogenization of irbesartan was carried out in the presence of poloxamer 407 at 6000 rpm for 2 h. Final nanosuspension preparation was done by media milling with zirconium dioxide beads. Here, the amount of poloxamer 407 and zirconium dioxide beads was studied as statistical independent variables. Response surface plot analysis and desirability function were applied to the selected optimized batch. The prepared batches were subjected to evaluation for zeta potential value, mean particle size, PDI, dissolution study, and stability study. Target particle size was less than 500 nm, and in vitro dissolution in 10 min was more than 80%. Zeta potential value was ~ 27 mV for optimized nanosuspension. Desirability of 0.941 was achieved. Checkpoint batch was prepared and evaluated to confirm the validity of mathematical model. Accelerated stability study was performed on the optimized batch at 40 ± 2 °C/75 ± 5% RH for 6 months.

Conclusion
The results confirmed the stability of formulation at accelerated stability conditions. Using presuspension prepared by homogenization, media milling time primarily reduced from 24–28 h to 18 h. Future perspective is to study other factors in combination method in discrete.