bluuebunny/crossref_metadata_2025_split huggingface dataset:
The effect of addition of bacterium Pseudomonas aeruginosa on biodegradation of methyl orange dye by brown-rot fungus Gloeophyllum trabeum
Authors: Purnomo A S , Rahmadini F D , Nawfa R , Putra S R 
Published: January 2021

Abstract
The methyl orange (MO), one of common textile dyes from azo groups, has negative impact in human life and the environment. Therefore, many attemps have been devoted to find the most effective method for MO degradation. Brown-rot fungus Gloeophyllum trabeum has identified as the biodegradable agent of MO, but its efficiency is still low, and it requires a long incubation time. In this work, the biodegradable performance of brown-rot fungus Gloeophyllum trabeum was investigated for MO degradation in the presence of bacterium Pseudomonas aeruginosa with various volumes (2-10 mL, 1 mL = 5.05 x 1012 Colony Forming Unit (CFU)). The addition of 10 mL of bacteria into G. trabeum culture showed the maximum degradation of 88.67% in potato dextrose broth (PDB) medium for the 7-day incubation. The identified metabolites were 4-((4-(dimethyliminio) cyclohexa-2.5-dien-1-ylidenehydrazinyl) phenolate (C14H15N3O, compound 1), 4-((4-iminiocyclohexa-2.5-dien-1-ylidene) hydrazinyl) benzenesulfonate (C12H10N3O3S, compound 2), 4-((hidroksi-4-iminioyclohexa-2.5-dien-1-ylidene) hydrazinyl) benzenesulfonate (C12H10N3O4S, compound 3), 4-((4-(dimethyliminio) hydroxy-cyclohexa-2.5-dien-1-ylidene) hydrazinyl) methoxy benzenesulfonate (C15H16N3O5S, compound 4), and 4-((4-(dimethyliminio) dihydroxy-cyclohexa-2.5-dien-1-ylidene) hydrazinyl) dimethoxy benzenesulfonate (C16H18N3O7S, compound 5). Based on the identification of metabolic products, the mixed cultures transformed MO via three pathways: (1) desulfonylation, (2) demethylation, and (3) hydroxylation. These results indicate that P. aeruginosa can enhance MO biodecolorization by G. trabeum.
Degradation of linear alkylbenzene sulfonate (LAS) using TiO2-chitosan composite as a photocatalyst
Authors: Rizky N A , Irawati U , Rohman T 
Published: January 2021

Abstract
Linear Alkylbenzene Sulfonate (LAS) is an anionic surfactant that is widely used as an active ingredient in synthetic detergent formulation. Being a component in domestic waste, LAS can potentially be harmful to the environment. In this research, the degradation of LAS using TiO2-chitosan composite as a photocatalyst was investigated. The optimum dose of TiO2-chitosan to be used was determined, and the effect of pH towards the degradation effectiveness was also studied. The results showed that a dose of 1 g/100 mL composite at pH 6 gave an optimum result for the LAS degradation. In this optimum condition, the decrease in LAS concentration was up to 95.20% from its initial concentration.
Comparison of ferrate (FeO4
2-) and ultrasonic waves ability as Coliform antibacterial in Kahayan River water Central Kalimantan
Authors: Kurniawati N , Sunariyati S , Karelius K , Hakim L , Krestina W , Wardani D A P 
Published: January 2021

Abstract
This study aims to compare ferrate (FeO4
2-) and exposure of ultrasonic waves as an antibacterial coliform in the Kahayan River water in Central Kalimantan. Sampling in this study used the Grab Sampling method with the Kemmerer Sampler tool. Ferrate (FeO42-) was made by reacting to a solution of Fe(NO)3 with NaOCl in alkaline conditions. Application of Ferrate (Fe(VI)) done by varying the concentration (0.03125; 0.0625; 0.125; 0.25; 0.5 mmol/L) to get the best concentration, and follow by varying times (0, 5, 10, 15, 20, 25, 30 minutes) at optimum concentration. Ultrasonic wave exposure was carried out with varying frequencies to get the optimum frequency and followed by variations in the exposure time at the optimum frequency. The coliform test was carried out by the MPN method with stages of detection tests, affirmation tests, and colony counting. The MPN analysis showed that ferrate could kill coliform bacteria at a concentration of 0.0625 mmol/L during the 5 minutes stirring time with 100% efficiency. Ultrasonic wave exposure could kill coliform bacteria optimally at a 40 kHz frequency during 3 hours of exposure time with 96% efficiency These results showed that ferrate was more effective in killing coliform bacteria compared with ultrasonic wave exposure.
Low concentration lead ion adsorption determination performance using activated carbon from bambu betung (Dendrocalamus asper)
Authors: Zakaria A , Yuliani N , Oktaviani A , Fachrurrazie  
Published: January 2021

Abstract
Adsorption is one alternative that is used to reduce lead (Pb) at low concentrations. Activated charcoal from Dendrocalamus asper (Schult) known as local name “Bambu Betung” has better performance in low concentration Pb adsorption at the liquid waste. The activation by added Na2CO3 was carried out for 24 hours and followed by neutralization of the filtrate until it reaches pH 7. Determination of optimum adsorption using adsorbent weight, adsorbate pH and contact time was treated concurrently including the design of surface method response statistics central composite design (RSM CCD). An optimal condition conducted to obtain these adsorption Langmuir and Freundlich isothermal mechanism. CCD processing was managed to obtained optimum conditions of pH, weight, and time. The results of the isothermal equation were in accordance with the Langmuir model because the absorption model has given an r value of 0.998 through the Langmuir equation y = 0.047x + 0.009. Overall, the optimum adsorption capacity of activated charcoal from D.asper (Schult) was performed at 21.28 mg per gram adsorbent. Based on the characteristics of betung bamboo activated charcoal test using SEM-EDX it was shown that there was no contamination of lead ion, this is the same as activated charcoal in general.
The implementation of green industry standard batik industry to develop eco-friendly
Authors: Indrayani L , Triwiswara M 
Published: January 2021

Abstract
Since UNESCO has established batik as Indonesia’s cultural heritage, batik has become increasingly recognized internationally. However, many parties are concerned about environmental problems due to the waste from the batik production process. Therefore, the batik industry must change its thinking about the concept of environmentally friendly industries by applying green industry principles. This paper will explain the application of green industry principles that can be fulfilled at every stage of the batik production process. The technical and management requirements are covered by the Green Industry Standard (GIS) for the batik industry which is stipulated by the Minister of Industry Regulation Number 39 of 2019. GIS includes the efficiency of input materials, namely raw and auxiliary materials as well as the selection of alternative materials that are more environmentally friendly, energy-efficient and minimize water consumption and pay attention to the principles of water conservation, good waste treatment, and limitation of air emissions due to the use of technology and low-carbon energy options. It is expected that with the application of green industry principles, the batik industry will not only experience an increase in the economic sector but become an environmentally industry.
The potential of food waste as bioplastic material to promote environmental sustainability: A review
Authors: Ramadhan M O , Handayani M N 
Published: January 2021

Abstract
Food waste is a challenge for sustainable development since it can increase greenhouse gas emissions and other environmental issues. Food processing and manufacturing are known to raise food waste. Meanwhile, plastic waste is a big problem in environmental pollution. This study aims to explore the potential of food waste as bioplastic material as an environmentally friendly alternative packaging. The method used was an effective literature review. The results showed that many food wastes have the potential to be developed as bioplastics if they contain biopolymers such as starch, cellulose, or other biopolymers. Some of the food wastes are obtained from the food processing industry such as sludge waste, cassava peel, banana peel, pineapple peel, durian seed, jackfruit seed, avocado seed, and chicken feather. The Development of bioplastics from food waste has a double benefit that can solve two problems indirectly, namely reducing plastic waste and food waste at the same time, thereby promoting environmental sustainability.
A review: The use of mangrove for biomonitoring on aquatic environment
Authors: Wilda R , Hamdan A M , Rahmi R 
Published: January 2021

AbstractMangroves have been investigated widely as plants that can absorb and accumulate heavy metals in their tissues. Due to that ability, mangroves have been used to reduce heavy metals in the aquatic environment. Furthermore, mangroves have been used for biomonitoring of heavy metals pollution. The use of mangroves for biomonitoring on aquatic environments has been considered as a cheap, rapid, and sufficient method. The mangrove is an organism that has the ability to absorb the contaminants of heavy metal and to function as fine pollutants trap. However, to develop this technique for wide use, research and investigation are still needed. This paper is aimed to describe the future direction of mangrove studies of using mangroves as a biomonitoring agent. Based on the review,Rhizophopora mucronataandAvicennia marinaare mangrove species that have a promising ability to be used for biomonitoring in the aquatic environment.
Biodegradation of buried crude oil in soil microcosm by fungal co-culture
Authors: Yanto D H Y , Hidayat A 
Published: January 2021

Abstract
Crude oil pollution in soil is a major environmental problem over the world. After long-term contamination, crude oil can be buried in the soil and leaving the toxic and persistence compounds. Treatment of buried crude oil in soil is a major challenge. In this study, buried crude oil in soil (20 cm-depth) was treated by the co-culture of three fungal strains (Pestalotiopsis sp. NG007/Polyporus sp. S133/Trametes hirsuta D7 with composition 1/1/1 and pre-grown in wood meal) placed on the top or bottom layer of the soil. Biodegradation behavior of crude oils were analyzed at different depth interval (0–5 cm, 5–15 cm, and 15–20 cm) with constant oxygen diffusion. The results showed that both position of fungal co-culture could degrade crude oil 63 – 92% after 30 days. Placing the co-culture in the bottom layer position of the soil demonstrated more effective degradation for all the depth interval than that of the top layer position. During the biodegradation, the co-culture produced oxidative enzymes such as catechol 1,2-dioxygenase, catechol 2,3-dioxygenase, laccase, manganese peroxidase, and lignin peroxidase with significant activities. This study offers an important strategy to remediate crude oil contaminated soil, specifically buried crude oil.
Network approach to mutagenesis sheds insight on phage resistance in mycobacteria
Authors: Sinha Saptarshi , Samaddar Sourabh , Das Gupta Sujoy K , Roy Soumen 
Published: January 2021

Abstract

Motivation
A rigorous yet general mathematical approach to mutagenesis, especially one capable of delivering systems-level perspectives would be invaluable. Such systems-level understanding of phage resistance is also highly desirable for phage–bacteria interactions and phage therapy research. Independently, the ability to distinguish between two graphs with a set of common or identical nodes and identify the implications thereof, is important in network science.

Results
Herein, we propose a measure called shortest path alteration fraction (SPAF) to compare any two networks by shortest paths, using sets. When SPAF is one, it can identify node pairs connected by at least one shortest path, which are present in either network but not both. Similarly, SPAF equalling zero identifies identical shortest paths, which are simultaneously present between a node pair in both networks. We study the utility of our measure theoretically in five diverse microbial species, to capture reported effects of well-studied mutations and predict new ones. We also scrutinize the effectiveness of our procedure through theoretical and experimental tests on Mycobacterium smegmatis mc2155 and by generating a mutant of mc2155, which is resistant to mycobacteriophage D29. This mutant of mc2155, which is resistant to D29 exhibits significant phenotypic alterations. Whole-genome sequencing identifies mutations, which cannot readily explain the observed phenotypes. Exhaustive analyses of protein–protein interaction network of the mutant and wild-type, using the machinery of topological metrics and differential networks does not yield a clear picture. However, SPAF coherently identifies pairs of proteins at the end of a subset of shortest paths, from amongst hundreds of thousands of viable shortest paths in the networks. The altered functions associated with the protein pairs are strongly correlated with the observed phenotypes.

Supplementary information
Supplementary data are available at Bioinformatics online.
Recovery after breast cancer surgery following recommended pre and postoperative physical activity: (PhysSURG-B) randomized clinical trial
Authors: Heiman J , Onerup A , Wessman C , Haglind E , Olofsson Bagge R 
Published: January 2021

Abstract

Background
The effect of preoperative physical activity on recovery and complications after primary breast cancer surgery is unknown. The objective of this trial was to evaluate whether a recommendation of non-supervised physical activity improved recovery after breast cancer surgery.

Methods
This parallel, unblinded, multicentre interventional trial randomized women in whom breast cancer surgery was planned. The intervention consisted of an individual recommendation of added aerobic physical activity (30 min/day), before and 4 weeks after surgery. The control group did not receive any advice regarding physical activity. The primary outcome was patient-reported physical recovery at 4 weeks after surgery. Secondary outcomes included mental recovery, complications, reoperations, and readmissions.

Results
Between November 2016 and December 2018, 400 patients were randomized, 200 to each group. Some 370 participants (180 intervention, 190 control) remained at 4 weeks, and 368 at 90 days. There was no significant difference in favour of the intervention for the primary outcome physical recovery (risk ratio (RR) 1.03, 95 per cent c.i. 0.95 to 1.13). There was also no difference for mental recovery (RR 1.05, 0.93 to 1.17) nor in mean Comprehensive Complication Index score (4.2 (range 0–57.5) versus 4.7 (0–58.3)) between the intervention and control groups.

Conclusion
An intervention with recommended non-supervised physical activity before and after breast cancer surgery did not improve recovery at 4 weeks after surgery. Registration number: NCT02560662 (http://www.clinicaltrials.gov).
Surgical outcomes after radioactive 125I seed versus hookwire localization of non-palpable breast cancer: a multicentre randomized clinical trial
Authors: Taylor D B , Bourke A G , Westcott E J , Marinovich M L , Chong C Y L , Liang R , Hughes R L , Elder E , Saunders C M 
Published: January 2021

Abstract

Background
Previous studies have suggested improved efficiency and patient outcomes with 125I seed compared with hookwire localization (HWL) in breast-conserving surgery, but high-level evidence of superior surgical outcomes is lacking. The aim of this multicentre pragmatic RCT was to compare re-excision and positive margin rates after localization using 125I seed or hookwire in women with non-palpable breast cancer.

Methods
Between September 2013 and March 2018, women with non-palpable breast cancer eligible for breast-conserving surgery were assigned randomly to preoperative localization using 125I seeds or hookwires. Randomization was stratified by lesion type (pure ductal carcinoma in situ (DCIS) or other) and study site. Primary endpoints were rates of re-excision and margin positivity. Secondary endpoints were resection volumes and weights.

Results
A total of 690 women were randomized at eight sites; 659 women remained after withdrawal (125I seed, 327; HWL, 332). Mean age was 60.3 years in the 125I seed group and 60.7 years in the HWL group, with no difference between the groups in preoperative lesion size (mean 13.2 mm). Lesions were pure DCIS in 25.9 per cent. The most common radiological lesion types were masses (46.9 per cent) and calcifications (28.2 per cent). The localization modality was ultrasonography in 65.5 per cent and mammography in 33.7 per cent. The re-excision rate after 125I seed localization was significantly lower than for HWL (13.9 versus 18.9 per cent respectively; P = 0.019). There were no significant differences in positive margin rates, or in specimen weights and volumes.

Conclusion
Re-excision rates after breast-conserving surgery were significantly lower after 125I seed localization compared with HWL. Registration number: ACTRN12613000655741 (http://www.ANZCTR.org.au/).
Sphincter-saving surgery after neoadjuvant therapy for ultra-low rectal cancer where abdominoperineal resection was indicated: 10-year results of the GRECCAR 1 trial
Authors: Rouanet P , Rivoire M , Gourgou S , Lelong B , Rullier E , Jafari M , Mineur L , Pocard M , Faucheron J L , Dravet F , Pezet D , Fabre J M , Bresler L , Balosso J , Lemanski C 
Published: January 2021

This phase III trial included patients with ultra-low rectal adenocarcinoma that initially required abdominoperineal resection. The surgical decision was based on clinical tumour status after preoperative treatment. The overall sphincter-saving resection rate was 85 per cent, with 72 per cent rate of intersphincteric resection. Long-term results showed that changing the initial abdominoperineal resection indication into a sphincter-saving resection according to tumoral response is oncologically safe.
Saving the sphincter
Laparoscopic Roux-en-Y gastric bypass versus laparoscopic sleeve gastrectomy: 5-year outcomes of merged data from two randomized clinical trials (SLEEVEPASS and SM-BOSS)
Authors: Wölnerhanssen B K , Peterli R , Hurme S , Bueter M , Helmiö M , Juuti A , Meyer-Gerspach A C , Slawik M , Peromaa-Haavisto P , Nuutila P , Salminen P 
Published: January 2021

Abstract

Background
Laparoscopic sleeve gastrectomy (LSG) and Roux-en-Y gastric bypass (LRYGB) are both effective surgical procedures to achieve weight reduction in patients with obesity. The trial objective was to merge individual-patient data from two RCTs to compare outcomes after LSG and LRYGB.

Methods
Five-year outcomes of the Finnish SLEEVEPASS and Swiss SM-BOSS RCTs comparing LSG with LRYGB were analysed. Both original trials were designed to evaluate weight loss. Additional patient-level data on type 2 diabetes (T2DM), obstructive sleep apnoea, and complications were retrieved. The primary outcome was percentage excess BMI loss (%EBMIL). Secondary predefined outcomes in both trials included total weight loss, remission of co-morbidities, improvement in quality of life (QoL), and overall morbidity.

Results
At baseline, 228 LSG and 229 LRYGB procedures were performed. Five-year follow-up was available for 199 of 228 patients (87.3 per cent) after LSG and 199 of 229 (87.1 per cent) after LRYGB. Model-based mean estimate of %EBMIL was 7.0 (95 per cent c.i. 3.5 to 10.5) percentage points better after LRYGB than after LSG (62.7 versus 55.5 per cent respectively; P &lt; 0.001). There was no difference in remission of T2DM, obstructive sleep apnoea or QoL improvement; remission for hypertension was better after LRYGB compared with LSG (60.3 versus 44.9 per cent; P = 0.049). The complication rate was higher after LRYGB than LSG (37.2 versus 22.5 per cent; P = 0.001), but there was no difference in mean Comprehensive Complication Index value (30.6 versus 31.0 points; P = 0.859).

Conclusion
Although LRYGB induced greater weight loss and better amelioration of hypertension than LSG, there was no difference in remission of T2DM, obstructive sleep apnoea, or QoL at 5 years. There were more complications after LRYGB, but the individual burden for patients with complications was similar after both operations.
Monitoring the evolving impact of COVID-19 on institutional surgical services: imperative for quality improvement platforms
Authors: Leung S , Al-Omran M , Greco E , Qadura M , Wheatcroft M , Mamdani M , Gomez D , de Mestral C 
Published: January 2021

The COVID-19 pandemic caused a massive slow down of scheduled surgical services, leading to concerns about increased morbidity from delayed care and of a large backlog of patients. In these times it is critical to collect and monitor data on surgical service volumes to help minimize the consequences to our patients. Here we describe our institutional vascular service volumes through the pandemic to date, demonstrating how quality improvement platforms can be useful tools for proactive data collection and monitoring..
Continued evaluation.
Scoring of tumour response after neoadjuvant therapy in resected pancreatic cancer: systematic review
Authors: van Roessel S , Janssen B V , Soer E C , Fariña Sarasqueta A , Verbeke C S , Luchini C , Brosens L A A , Verheij J , Besselink M G 
Published: January 2021

Abstract

Background
Preoperative chemo(radio)therapy is used increasingly in pancreatic cancer. Histological evaluation of the tumour response provides information on the efficacy of preoperative treatment and is used to determine prognosis and guide decisions on adjuvant treatment. This systematic review aimed to provide an overview of the current evidence on tumour response scoring systems in pancreatic cancer.

Methods
Studies reporting on the assessment of resected pancreatic ductal adenocarcinoma following neoadjuvant chemo(radio)therapy were searched using PubMed and EMBASE. All original studies reporting on histological tumour response in relation to clinical outcome (survival, recurrence-free survival) or interobserver agreement were eligible for inclusion. This systematic review followed the PRISMA guidelines.

Results
The literature search yielded 1453 studies of which 25 met the eligibility criteria, revealing 13 unique scoring systems. The most frequently investigated tumour response scoring systems were the College of American Pathologists system, Evans scoring system, and MD Anderson Cancer Center system, investigated 11, 9 and 5 times respectively. Although six studies reported a survival difference between the different grades of these three systems, the reported outcomes were often inconsistent. In addition, 12 of the 25 studies did not report on crucial aspects of pathological examination, such as the method of dissection, sampling approach, and amount of sampling.

Conclusion
Numerous scoring systems for the evaluation of tumour response after preoperative chemo(radio)therapy in pancreatic cancer exist, but comparative studies are lacking. More comparative data are needed on the interobserver variability and prognostic significance of the various scoring systems before best practice can be established.